LOCUS       DATASH010000001         2978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2769352,
            whole genome shotgun sequence.
ACCESSION   DATASH010000001 DATASH010000000
VERSION     DATASH010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2769352"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..297)
                     /locus_tag="VHV99_00005"
                     /pseudo
     CDS             complement(1..297)
                     /locus_tag="VHV99_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420431.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            complement(310..480)
                     /locus_tag="VHV99_00010"
     CDS             complement(310..480)
                     /locus_tag="VHV99_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3633885.1"
                     /translation="MKIVVRIELITDWGDVNTIEVGRIDRPSQTLDQESVGLSLAPVQ
                     IGEASRLSSGFD"
     gene            663..911
                     /locus_tag="VHV99_00015"
     CDS             663..911
                     /locus_tag="VHV99_00015"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEX3633886.1"
                     /translation="MSTSSYTEAEYRNFLGVQPDLFLAAQDACRFAVEALTRSLGVEP
                     YEAYSIMGMVGELRIHEIVDRPNWVVGCMLPRTLFARG"
     gene            complement(1007..1846)
                     /locus_tag="VHV99_00020"
     CDS             complement(1007..1846)
                     /locus_tag="VHV99_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HEX3633887.1"
                     /translation="METSSGVGRGRLDCVERIFGRARGVVIGVIHCPAFPGSPRYQGA
                     ASEALYATARADALAYASGGVDGLIIENHGDVPFSKPDDIGHETSAFMAVTADRIARE
                     TGLPIGINVLANAAIPALAIAAASQAAFVRVNQWANAYVANEGLIEGAAANALRYRAR
                     LRADGISVFADAHVKHGAHAIVADRALEELVRDVEFFDADAIIATGQRTGDAASPDYL
                     RQIRAATTLPLLVGSGCTESNVAEILSIVDAVIVASSLKVGGVWWNPVDEDKVRAFMQ
                     AAR"
     gene            complement(1853..2815)
                     /locus_tag="VHV99_00025"
     CDS             complement(1853..2815)
                     /locus_tag="VHV99_00025"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX3633888.1"
                     /translation="MSTAVSPTPRICVVGNAAIDLSLNVARLPSPGETTLALDSTSGF
                     GGKGANQAVAAARAGASTLLFAAVGEDADGDRLIAQLNAEGIDTRHLLRMPCPTDLSI
                     VTVDAAGENTIVTRNLAAANYRPDRCALHEATRDGDWIALQGNLSADVTAEVLRTARL
                     GACRTLLNPGPVRFDCMPLLADLDVLVVNRVEAATLTALDDPALAAAALHRAGAHDVC
                     VTLGADGVLWCDNHGTRHLAAVPAEAVDTVGAGDAFCGTLLAGLALNLPMPNALRWAQ
                     AVAAYAVARPGAQASFPERAAMRGLLHAAVPQCVVSSVTYSQAR"
BASE COUNT          540 a          979 c          923 g          536 t
ORIGIN      
        1 taaagataga cccatttgcc acggactttc acatacgttt cgtcgactct ccatgaacga
       61 ccagcggatt ttgcaaaccg gttccagcgc ttgacgaact ccggcgtgaa gcgtttgacc
      121 cagcgcatga tcgtagtgtg cgcgagcgac agcccgcgct cggccattat ttcaacgagg
      181 tctcaaaagc tgagcttgta acgcaggtac caacggtacc aacgcacaca ggacgatgac
      241 ttcgcggtaa aagtggcggc cgttgaacag cctgtcgatg tctgtcagct tactcatagc
      301 ctgcgcgcgc tagtcaaaac cggagcttaa cctactggct tctccgattt gcaccggagc
      361 caatgagagc cctacagatt cttgatcgag cgtttggctt ggccggtcga tgcggcccac
      421 ctcgatcgta tttacatcac cccagtcggt aatcagttcg atgcgaacga cgattttcat
      481 cgcggacctc gatgtaggta ctggcgaggt tcaatgctag cgctgccgaa aaccccacgc
      541 cttttaacag ttccttcgga atgacgcaaa gttgtccagc actgctcgct gtgccttata
      601 aactggtctt tgggtccgga cgactttgtg tcatttgaca actgctcgcg ccggtgctcg
      661 atatgtccac atccagctat accgaagccg agtatcgtaa cttccttgga gtccaacccg
      721 atctgtttct tgcggcccag gatgcgtgcc gctttgcggt agaggccctg acccgcagtc
      781 tcggcgtcga accctacgag gcgtattcca tcatgggtat ggtcggggag ttgaggatcc
      841 acgagatcgt cgacagaccg aactgggtcg tcggctgcat gctgccgcgt accttgttcg
      901 cgcgtggcta gagcgcgtta ccgcaactgg cggtccgcgg cgcctaaaaa gcaccccggg
      961 cccatgtccc ttcgtcgcga gtcgacgccg gcgaggagca cgagcactac cgcgccgcct
     1021 gcataaacgc ccgcactttg tcctcgtcga ccgggttcca ccacacgccg cccaccttca
     1081 acgaactggc gacgatcacg gcgtcgacaa tcgacagaat ctccgccacg ttgctctccg
     1141 tgcagccact gcccaccagc agcggcaagg tcgtcgccgc acgaatctgc cgcagatagt
     1201 ctgggctggc ggcgtctccc gtacgttgac cggtggcgat gatcgcgtcg gcgtcgaaga
     1261 attccacgtc gcgcaccagt tcttccagcg cgcgatccgc cacgatggca tgcgcgccgt
     1321 gcttgacgtg cgcatctgcg aacacgctga tgccgtccgc gcgcagccgc gcccgataac
     1381 gcagcgcgtt ggccgccgcg ccttcgatca gcccttcgtt ggcgacatac gcattggccc
     1441 attgattgac gcgcacgaag gccgcctggc tcgccgccgc gatagcgagc gcgggaatcg
     1501 ccgcattcgc cagcacgttg atgccgatcg gcagtccggt ttcgcgtgcg atgcgatccg
     1561 ccgtcaccgc catgaacgcc gaggtctcat gaccgatatc gtcgggcttc gagaatggca
     1621 cgtcaccgtg attttcgatg atcaggccgt cgacgccgcc gctcgcatac gcgagcgcat
     1681 cggcacgcgc ggtggcgtac agcgcttcgc tcgcggcacc ctgatagcgc ggcgagccgg
     1741 gaaaagccgg acagtgaatc acgccgatca ccacgccgcg cgcgcggcca aaaatccgct
     1801 cgacgcagtc gagacgcccg cggccaacac cagaagaagt ttccatatcg attcaacgag
     1861 cctgagagta ggtgactgac gaaacaacac actgcgggac tgccgcgtgc agcaacccgc
     1921 gcatcgcggc gcgctcggga aagctcgctt gcgcgccggg tcgggcgacc gcgtaagcgg
     1981 ccaccgcctg cgcccagcgt aacgcgtttg gcatcggcag gttgagcgcc agcccggcga
     2041 gcagcgtgcc gcaaaaggcg tcgccggcgc cgactgtatc gacggcttca gcaggcactg
     2101 ctgcgagatg ccgcgtgccg tggttatcgc accaaagtac gccgtccgcg ccgagcgtga
     2161 cgcagacgtc gtgcgcgccg gcgcgatgca gcgcggcagc ggcgagcgct gggtcgtcca
     2221 gcgcggtcaa cgtggcggct tcgacgcgat tgaccacgag cacatccagg tccgccagca
     2281 agggcatgca atcgaaacgg accggcccag gattgagcag cgtgcggcac gcaccgagcc
     2341 gcgcggtgcg caggacctcg gcggtgacgt cggcgctcag gttgccttgt agcgcgatcc
     2401 agtcgccgtc gcgcgtggct tcgtgcaacg cacagcggtc cgggcgatag ttggccgcag
     2461 ccaggttgcg cgtgacgatg gtgttttcgc cggcggcatc caccgtgacg atggacagat
     2521 cggtcgggca cggcatgcgc agcagatggc gcgtgtcgat gccttcggcg ttcagttgcg
     2581 cgatcagccg gtcgccgtcc gcgtcttcgc cgacggcggc aaacagcagc gtcgatgccc
     2641 ccgcgcgcgc cgcggccacg gcctggttcg cacccttgcc gccgaagccg gacgtgctgt
     2701 ccaaagcaag cgtggtttcg ccgggcgacg gcaaacgcgc gacgttcagg ctcagatcga
     2761 tcgccgcatt gccgaccacg cagatgcgcg gcgtaggact gacggctgtg ctcatcgccc
     2821 cgcccaatcg gtcagggcgc cgcgatacag atcgaccgct tccagcagcc gatcccattg
     2881 ctcgtcgccg ggcgggttga ccgtgtcggc ggcgaccagc ttgcgagttg gttcgataag
     2941 ctcgagtgcg tgcatggtgg cccttgcgcc tcttgcgg
//
LOCUS       DATASH010000002         6738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6132268,
            whole genome shotgun sequence.
ACCESSION   DATASH010000002 DATASH010000000
VERSION     DATASH010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6738
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6132268"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..102
                     /locus_tag="VHV99_00030"
     CDS             <1..102
                     /locus_tag="VHV99_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone"
                     /protein_id="HEX3633889.1"
                     /translation="APAATSVSSAPAATVKTALNPAAAWPFPTGSRP"
     gene            complement(373..849)
                     /locus_tag="VHV99_00035"
     CDS             complement(373..849)
                     /locus_tag="VHV99_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEX3633890.1"
                     /translation="MRITSRLMVAALIAGSLAMSGCAYNSSSADVYTASQAQREETVR
                     MGTVDSVRAVKISSNNGQPSGLGAIGGGALGAVAGSAIGGGRGSIVTGIIGGLAGAVA
                     GNAVENGVAVHDGLEITVRLDNGDMRAITQSATGEIFRAGERVRLLSSGGVTRVTH"
     gene            complement(878..1813)
                     /locus_tag="VHV99_00040"
     CDS             complement(878..1813)
                     /locus_tag="VHV99_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEX3633891.1"
                     /translation="MATLICGSLAYDNIMTFEGRFRDHILPEQVHILNVSFLVPTMRR
                     EFGGCAGNIAYSLHLLGGDARIMGTLGETDAQLYMDRLAQLGLSTENVLVVPGTYSAQ
                     AMITTDLENNQITAFHPGAMMQSHLNRADQAKDITLGIVGPDGYDGMIQHSEHLAAAG
                     VPFIFDPGQGLPLFDGESLRRMIELATYVAVNDYEAKLVSNKTGWSIEEIAGKVDALI
                     VTLGEQGAQIHHAGGIEAIPAVKAQQVLDPTGCGDAFRGGLLYGIENKLGWATTGRLA
                     SLMGALKIEHQGPQNYAPSRAQINERFKQAFGYDL"
     gene            complement(1935..2435)
                     /gene="tpx"
                     /locus_tag="VHV99_00045"
     CDS             complement(1935..2435)
                     /gene="tpx"
                     /locus_tag="VHV99_00045"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006765230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEX3633892.1"
                     /translation="MSQVTLGGNPIEVAGTFPSVGQKAPAFSLVGKDLKTLSLADFAG
                     KRKVLNIVPSLDTPTCATSTRKFNEAAAKLSNTAVIVVSGDLPFAASRFCTTEGIENV
                     VTASTFRGHKFAQAYGVDVTSGPLTGLTARAVVVLDENDKVVHAELVGEIKNEPNYDA
                     ALAALK"
     gene            complement(2509..3879)
                     /locus_tag="VHV99_00050"
     CDS             complement(2509..3879)
                     /locus_tag="VHV99_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon and DUF3426 domain-containing
                     protein"
                     /protein_id="HEX3633893.1"
                     /translation="MLLATRCPFCDTVFRLQPAQLALRRGLVRCGHCHEVFDASSSLF
                     ELNEDGDFSSAKPVAAAAAIEALSGVRPKGPDFSAEAWDPWAPAPDATIDNRLLHNAS
                     NLPLTPVSTDAGVAVTPRHATEPELPASAFTTPIPSDHEPILADAPLDPFAYEVDAPP
                     DIPREPFAYQAEPPAEQAPRVWHKTERPSEHLADTDEPVLKEPATLRGIPDNEPHFGA
                     AGLGVPGSGAAGLSAAATGAAASGAASAGGLDAGGGSGTAVPGGPATLGSSGEPFAVQ
                     PVHEGVNPFPVVRETRPAEPRRVGWIIFGSVIAVLLVIALLAQLAWWQRETVQVYFPR
                     SQTLYAKACAQLGCQITPPHDIDGLQVEPSDLRQIDGPHKLELKMPLRNRFNVALAYP
                     AIELTLLDDQNNVAVRRVLWPQDYVPPGTPIAAGLPAHTTQTMIVHLDTGNAVASNFR
                     VQIFYP"
     gene            complement(3928..4830)
                     /gene="prmA"
                     /locus_tag="VHV99_00055"
     CDS             complement(3928..4830)
                     /gene="prmA"
                     /locus_tag="VHV99_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX3633894.1"
                     /translation="MSYRELIAELAREHAEEFSDALLELGALSVSVEDADADTPDEQP
                     LFGEPGLTPDRTAWQRSRVIALLAPEHEPAVLLTAAANEIGLETAPSFTVREVEEQDW
                     VRLTQSQFDPIPIGERIWVVPSWHDAPDPDALVLELDPGLAFGTGSHPTTRLCMEWLE
                     QSVKPEQSVLDYGCGSGILAILAKKCGANPVYGIDIDPQAVESARHNSERNRAEVTYG
                     LPDECPTGEFDIVVANILSNPLKLMASMLSSKVKPGGRIALSGILARQADEVAQVYAR
                     WIDIGVWREHEGWVCLTGTRRESH"
     gene            complement(4834..6201)
                     /gene="accC"
                     /locus_tag="VHV99_00060"
     CDS             complement(4834..6201)
                     /gene="accC"
                     /locus_tag="VHV99_00060"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017777083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX3633895.1"
                     /translation="MFEKILIANRGEIALRIQRACRELGVKTVVVYSEADKEAKYVKL
                     ADEAVCIGPAPSNLSYLNMPALISAAEVTDAEAIHPGYGFLSENADFAERVEQSGFTF
                     IGPRPETIRMMGDKVTAKQTMIKTGVPCVPGSEGALPEDPKEIVKIARQVGYPVIIKA
                     AGGGGGRGMRVVHTEAALVNAVNMTREEAGRAFGNPQVYMEKFLENPRHIEIQILADS
                     FKNAVWLGERDCSMQRRHQKVIEEAPAPGIARRLIDRIGDRCADACKKMGYLGAGTFE
                     FLYENGEFYFIEMNTRVQVEHPVTELITGVDIVQEQIRIAAGEKLAFRQRDIVFKGHA
                     IECRINAEDPFKFIPSPGRLTSWHMPGGPGIRVDSHAYNGYFVPPNYDSMIGKLIAYG
                     ATREQAIKRMRIALSEMVVEGIQTNIPLHRELMLDAKFVEGGTSIHYLENRLAAKQQA
                     APEEA"
     gene            complement(6323..6736)
                     /gene="accB"
                     /locus_tag="VHV99_00065"
     CDS             complement(6323..6736)
                     /gene="accB"
                     /locus_tag="VHV99_00065"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEX3633896.1"
                     /translation="MSELEVTEGEGKVRIVKNAPPVYVQPSASYAPQYAQPAPLPGGE
                     APVAAAPATPAAAAPQGHVVTSPMVGTFYRAPSPGADPFVQVGDTVKEGQTICIIEAM
                     KLLNEIESDKSGVVKEILVENGQAVEYGQPLFVVG"
BASE COUNT         1155 a         2104 c         2183 g         1296 t
ORIGIN      
        1 gcacccgctg cgacttctgt ctcgagcgca ccggcggcga cggtgaagac cgcgctgaac
       61 ccggcagcgg catggccgtt cccgacgggc agccgtccgt aactggcgca taagcggtag
      121 aagtatttcg acacgtcgta ccgtgtgtcg atgatcgagt agcgcttaaa gtatgaccga
      181 gcgctactcg gtcagggtcc cgttgccggg tttctggcga cgggattttt tttggggctt
      241 cactcacgca aaagcctccc caacgaacga ctccaaaacc cgcaggcgaa aaaaaacgca
      301 tgcacagggg agcgcatgcg tgtgaggtcg tcgcggcggc gcgtgaagcg ccaaccgcgc
      361 gagaggggaa acttagtgtg tgacgcgcgt gacgccgccg ctggagagca gacggacgcg
      421 ttcgccggcg cggaagattt cgccggtggc gctttgcgtg atcgcgcgca tgtcgccgtt
      481 atcgagtcgc acggtgatct cgaggccgtc atgcaccgcc acgccgttct cgaccgcatt
      541 gcctgccacc gcgccagcca ggccgccgat gatgcccgtc acgatcgagc cgcggccacc
      601 gccgatcgca ctgccggcca ccgcgcccaa cgcgccgccg ccgatcgcgc cgaggccgct
      661 gggttggccg ttgttcgaac tgatcttcac ggcccgaacg ctgtcgactg tgcccatgcg
      721 aaccgtctct tcgcgctgcg cttgcgacgc tgtgtagaca tcggcagagc tgctgttgta
      781 cgcacagccc gacatggcca gtgagccggc aatcaaggcg gccaccatca ggcgactcgt
      841 tattctcatt ccctaactcc aatagcttgc ggtattacta caggtcgtat ccgaacgcct
      901 gcttgaaccg ttcgttgatc tgcgcccggc tgggcgcata gttttgcggg ccctggtgtt
      961 cgatcttgag cgcgcccatc aggctcgcca gacgcccggt ggtcgcccag ccaagcttgt
     1021 tctcgatgcc gtacagcaag ccgccgcgga acgcgtcgcc gcagcctgta ggatccagca
     1081 cctgctgtgc cttgaccgcg ggaatcgctt cgatgccgcc ggcgtgatga atctgcgcgc
     1141 cttgttcgcc gagcgtgacg atcagcgcgt cgaccttgcc cgcgatctct tcgatcgacc
     1201 aacctgtctt gttgctcaca agtttggctt cgtaatcgtt gaccgctacg taagtagcaa
     1261 gttcaatcat tcggcgcagc gactcgccgt cgaacagcgg caaaccttgg cccggatcga
     1321 agataaacgg cacgccggcg gccgccagat gctccgaatg ctggatcatg ccgtcgtagc
     1381 cgtcaggccc gacgatgccg agcgtgatgt ccttcgcttg atcggcgcga ttcaggtgag
     1441 actgcatcat cgcgcccggg tggaacgccg tgatctgatt gttttccagg tcggtggtga
     1501 tcatcgcctg ggccgagtag gtgcccggca ccaccagcac gttttccgtc gacagcccga
     1561 gctgcgcgag ccggtccatg tagagttgcg catcggtctc gccgagcgtc cccatgatgc
     1621 gtgcatcgcc gcccagcaga tgcagcgagt aagcgatgtt gcccgcgcag ccgccgaact
     1681 cgcggcgcat cgtcggcacg agaaagctca cgttcaggat atggacctgc tccggcagga
     1741 tgtgatcccg aaagcggcct tcgaaggtca tgatgttgtc gtaggcgagc gaaccgcaaa
     1801 tcagcgtagc caaggcgagc cttcctgtaa aaatagagag aatgggcgcg acgatgtgac
     1861 agcgccgcgc atgaagcgcg gcgctggggt tcgtgcgcga aagcttattt caatgcgggt
     1921 tacttcagtg cgggttactt cagtgcggcc agtgcggcgt cgtagttcgg ctcgttcttg
     1981 atttcgccga ccagttcagc gtgcacgacc ttgtcgttct cgtcgagcac caccacggcg
     2041 cgtgccgtca gacccgtcag cgggccgctc gtcacgtcga cgccataggc ttgcgcgaac
     2101 ttatggccgc ggaacgtgga cgccgtcacg acgttctcga tgccttcggt cgtgcagaag
     2161 cgcgacgcgg cgaacggcag atcgcccgac acgacgatca ctgccgtgtt gctcagcttg
     2221 gctgcggctt cgttgaactt acgggtcgac gtggcgcagg tcggcgtgtc gaggctcggg
     2281 acgatgttca gtaccttgcg cttgccggcg aaatcggcaa gcgacaaagt cttcagatcc
     2341 ttgccgacca gcgagaaggc gggcgctttc tggcccaccg acgggaaggt gccggctact
     2401 tcgatcgggt tgccacccag cgtgacttga ctcatgttgt tgtgctccga aaattcgtca
     2461 gtaatgacgg tgaatacgcc cagtcagtgc cgggcgcgcg aggacgcgtt acggataaaa
     2521 aatctgtacg cggaaattgg aagcgaccgc attgcccgtg tcgagatgga cgatcatggt
     2581 ctgggtcgtg tgcgccggca ggccggctgc gatcggcgtg ccgggtggga cgtagtcttg
     2641 cggccacagc acgcggcgca cggcgacatt gttctggtcg tcgagcagcg tgagttcgat
     2701 ggccgggtag gccagcgcca cgttgaagcg gttgcgcagc ggcatcttca gttcgagttt
     2761 gtgcgggccg tcgatctgcc gcagatcgga cggttcgacc tgcaagccgt cgatgtcgtg
     2821 cggcggcgtg atctggcagc ccagctgcgc gcaggctttt gcatagagcg tctgcgaacg
     2881 ggggaagtag acctgcacgg tttcgcgctg ccaccaggcg agttgcgcga gcaaggcgat
     2941 caccagcagc actgcgatca ccgagccgaa gatgatccag ccgacgcggc gcggttcggc
     3001 ggggcgcgtt tcacgtacca ccgggaaggg attgacgcct tcatgcacgg gctgaaccgc
     3061 gaacggttcg ccgctcgatc ccaatgtggc cgggccaccg gggacggccg tgcctgagcc
     3121 gccgcctgcg tccaggccac ccgcgctggc cgcacctgag gccgccgcac ctgtggcggc
     3181 tgcgctcaag ccggctgcgc ccgagcctgg cacgcctaag ccggccgcgc caaaatgcgg
     3241 ctcattgtca ggaatgccac gcaaagtcgc cggctccttc aaaaccggtt cgtcagtatc
     3301 ggcaagatgt tcagagggac gctcggtctt gtgccagacc cgcggcgctt gttccgcagg
     3361 cggctcggcc tgataagcga acggctctct cgggatgtca ggcggcgcat cgacttcgta
     3421 ggcgaacgga tcgagcggcg cgtccgcgag gatgggctcg tggtcggaag gaatcggcgt
     3481 ggtgaaggcg ctcgccggca gttccggctc ggtggcgtgg cgcggcgtga ccgcgacgcc
     3541 cgcatcggtg gagaccggcg tgagcggcag attgctggcg ttgtgaagca ggcggttgtc
     3601 gatcgtagcg tccggcgccg gtgcccacgg gtcccatgct tcggcgctga aatcggggcc
     3661 cttgggacgt acgccggaca acgcctcgat tgccgcagcc gcggcaaccg gcttggcgga
     3721 cgagaagtcg ccgtcctcat tcagttcgaa caggctgctc gatgcgtcga atacttcatg
     3781 gcaatgcccg cagcgcacga ggccacggcg cagcgcgagc tgcgccggtt gcaggcggaa
     3841 gacggtatcg cagaaggggc aacgcgtcgc caggagcata ttgagccggt gagccgaagg
     3901 gctgttggtt ggacaatacg ccttattcta atggctttcg cggcgcgttc ccgttagaca
     3961 tacccaacct tcgtgttcgc gccatacgcc gatgtcgatc caccgtgcgt agacctgcgc
     4021 cacttcgtcc gcttgccgcg ccagaatgcc ggacagtgcg atacggccgc ccggcttgac
     4081 cttcgacgac agcatcgagg ccatcagctt gagcggattg gacaggatat tggcgaccac
     4141 gatatcgaac tcgccggtgg ggcattcgtc aggcaagccg taggtgactt ctgcgcggtt
     4201 gcgctcgctg ttgtgacgcg ccgattcgac cgcttgcggg tcgatgtcga tgccgtacac
     4261 cgggttggcg ccgcacttct tcgcgaggat cgcgaggatg cccgagccgc agccgtagtc
     4321 gagcacggat tgctccggct tgaccgattg ttccagccat tccatgcaca gacgcgtggt
     4381 gggatggctg ccggttccaa aggcgagacc gggatcgagt tccagcacga gcgcgtcagg
     4441 gtccggtgca tcgtgccacg agggcacgac ccagatgcgc tcgccgatcg gaatcggatc
     4501 gaattgcgac tgcgtcagac gcacccagtc ctgctcttcg acttcacgca cggtgaacga
     4561 tggtgcggtt tccagcccaa tctcattggc cgcggccgtg agcagcaccg ccggttcgtg
     4621 ttccggcgcg agcagcgcga tcacgcgcga gcgttgccac gccgttcgat ccggcgtgag
     4681 accgggctcg ccgaacagcg gctgctcgtc gggcgtgtcg gcgtccgcat cttcaaccga
     4741 tacggacaac gcacccaact cgagcagcgc gtcggagaac tcctccgcgt gctcgcgtgc
     4801 cagttcggcg atcagttccc ggtagctcat gagttacgct tcttccggtg cggcttgctg
     4861 cttcgcggcc aaccggtttt cgaggtagtg aatgctggtg ccgccttcga cgaatttcgc
     4921 gtccagcatc agttcgcggt gcagcgggat gttggtctga atgccttcca ccaccatttc
     4981 cgacagtgcg atgcgcatcc gcttgatcgc ctgctcgcgc gtcgcgccgt aagcgatcag
     5041 cttgccgatc atcgaatcat agttcggcgg cacgaaatag ccattgtagg cgtgcgagtc
     5101 gacacggatg ccggggccgc ccggcatatg ccacgacgtc aaacgtcccg gcgacggaat
     5161 gaacttgaac ggatcttcag cattgatccg gcattcgatc gcatgaccct tgaacacgat
     5221 gtcgcgttga cggaacgcga gcttctcgcc ggccgcgata cggatctgtt cctgcacgat
     5281 gtcgacgccc gtgatcagct cggtcaccgg atgctcgacc tgcacgcgcg tgttcatttc
     5341 gatgaagtag aactcgccgt tttcgtacag gaactcgaac gtacccgcgc caagatagcc
     5401 catcttcttg caggcgtccg cgcagcgatc gccgatgcgg tcgatcaggc ggcgcgcgat
     5461 gcccggcgcc ggcgcttctt cgatcacttt ctgatgacgg cgctgcatcg agcaatcgcg
     5521 ctcgccgagc cagaccgcgt tcttgaacga gtcggccagg atctgaattt cgatgtgccg
     5581 cgggttctcc aggaatttct ccatgtagac ctgcgggttg ccgaatgcac ggccggcttc
     5641 ttcgcgcgtc atgttgaccg cgttgaccag cgccgcttcc gtgtggacca cgcgcatgcc
     5701 gcgaccgccg ccgccgccgg cggccttgat gatcacgggg tagccgacct ggcgggcaat
     5761 tttcacgatc tctttcggat cttccggcaa tgcgccttca gaacccggca cgcagggcac
     5821 gccggtcttg atcatggttt gcttggcggt caccttgtcg cccatcatcc ggatggtttc
     5881 cgggcgcgga ccgatgaagg tgaagccgga ctgctcgacg cgttcggcga agtcggcgtt
     5941 ttccgacagg aagccgtagc cggggtggat tgcttcggcg tccgtcactt ctgccgcgct
     6001 gatcagcgcg ggcatgttca ggtagctcag attcgaaggc gccgggccga tacagaccgc
     6061 ctcgtcggcg agcttcacgt acttggcttc cttgtcggct tccgaataga cgacgacggt
     6121 cttgacgcca agttcgcggc aggcgcgctg gatgcgaagc gcgatctcgc cacgattggc
     6181 aatgaggatt ttttcaaaca tagcgggttt tcgactcatc aataggcacc cggttgattc
     6241 acaacgcagg ctgcctcaga ggatcagtcg cgcgcctcgg gagggcaggc gcgcgggcgg
     6301 caggcgcaaa gcgtgccgcg cgttagccga ccacgaacaa cggttggccg tactcgaccg
     6361 cctggccgtt ctcgacgagg atttccttta ccacgccgga cttgtccgac tcgatttcgt
     6421 tgagcagctt catcgcttcg ataatgcaga tcgtctggcc ttccttgacc gtgtcgccta
     6481 cctggacgaa cgggtccgcg cccggcgacg gtgcgcggta gaacgtgccg accatcggcg
     6541 aggtcaccac gtggccttgc ggcgcggcgg cggccggcgt agccggtgcg gctgcgaccg
     6601 gggcttcacc gcctggcaga ggagccggct gggcgtattg cggcgcatac gaagcggacg
     6661 gttgcacgta aaccggcggc gcattcttga cgatgcggac cttgccttca ccttcggtca
     6721 cttcgagttc ggaaatac
//
LOCUS       DATASH010000003         5068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2967465,
            whole genome shotgun sequence.
ACCESSION   DATASH010000003 DATASH010000000
VERSION     DATASH010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5068
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2967465"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="VHV99_00070"
     CDS             <1..787
                     /locus_tag="VHV99_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CobT"
                     /protein_id="HEX3633897.1"
                     /translation="RADTAPLKEKLERALSADERTRWRREQERGEIDRTSLAKLATSP
                     GYRTPFRTQRAAKWRDVAVTLLIDRSGSMAGRKIELARLCATALCDALTQLSFDCEVL
                     GYCSLESAPMKQLYERQRAAGADLGRYNRLVERLDLKVYKRFGAIDLSGIAAIDCGHE
                     NPDGEALAWAATRLADHQAERRILIVFSDGYPSTGDGDPQVLRSDLRERVAAIQTRGI
                     ELVGIGVLTDAVEDFYPHNVVVSRLAELPSTVFSVLSSMLLTR"
     gene            complement(857..1690)
                     /locus_tag="VHV99_00075"
     CDS             complement(857..1690)
                     /locus_tag="VHV99_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007589735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEX3633898.1"
                     /translation="MCRWLAYTGNPLQLETVLFNAKHSLIDQSLHSRLGHTTTNGDGF
                     GIGWYGHPTDIPFRYRCIQPAWSDRNLREAARAIRAPLFVAHVRAATDTPSQETNCHP
                     FRHGRWLFAHNGLVRDYPKVRRDLMLAIDPELFPSIEGSTDSEVLFFLALTFGLELAP
                     VTAIERMVGFVEDIGRKHGVDEPLNMTVCATDGEQIVAVRYSSEGQSRSLFHSSSFRQ
                     LHELYPDDPRIVAAGEDAFLVLSEPLADLPGWWQEIPESTAVVARGGEIAQYPFEPKL
                     V"
     gene            complement(1764..2441)
                     /locus_tag="VHV99_00080"
     CDS             complement(1764..2441)
                     /locus_tag="VHV99_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEX3633899.1"
                     /translation="MVDSLISDILFGFTGLISIINPIGMAFVFLDRTASLTTEDRTAL
                     ARKIAFNVMCVMLVAFFVGTPILHFFGISMQALRIGGGLAVAVGGWQMLNAPDTQPAE
                     QPAVKRVDSDNAMSKAFFPLTIPLTTGPGSIATAIALTANRTHKLSAFVLSSIASVVI
                     SILVAATVYLIYSRAVVFARYLGVDGTKVAMRVSSFLLLCIGVQIILTGFSEFLIPIA
                     QMQPVVK"
     gene            2788..3003
                     /locus_tag="VHV99_00085"
     CDS             2788..3003
                     /locus_tag="VHV99_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3311 domain-containing protein"
                     /protein_id="HEX3633900.1"
                     /translation="MLLRVLAALPFVGMLLGVPFVNRVEPLVLGMPFVLAWIVMWVVL
                     SSIIMAIVYRLDPTNRQLAVDGEEGRP"
     gene            3000..4475
                     /locus_tag="VHV99_00090"
     CDS             3000..4475
                     /locus_tag="VHV99_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEX3633901.1"
                     /translation="MSALVIITAITLFALYLGVRARRGHDMNLEQWTVGGRSFGTAFV
                     FLLMAGEIYTTFTFLGGSGFAYGKGAPVYYILAYGTLAYIISYWMLPPIWRYAKQHRL
                     VSQPHFFARKYDSPALGTLVALVGVAALIPYLVLQLKGLGIIVATASYGAISSTAAVW
                     IGAGVVTSYVIVSGVRGSAWNSVVKDMLILAIVLFLGIYLPLHYYGGFSDMFRAIDAA
                     RPGFLTFPAKGSSVTWFQSTVLLTALGFFMWPHTFGSIFTAKDERIFRRNAMVLPLYQ
                     LILLFVFFVGFAATLKVPGLKGGDIDLSLFRLSLQTFDPWLVGVIGAAGILTALVPGS
                     MILTSASTLLANDVYRGMVSRNASDATVTKLARFLVPVVALVAVGFTLHGGETIVALL
                     LMGYNFVTQLFPAVICSLLPKNRATKQGAFCGILAGVAVVAVTTMMHLSIGQLMPFLS
                     DALKDVNIGFLALAVNVVVFAVVSAVTRPRAVEGAQASSVH"
BASE COUNT          858 a         1476 c         1639 g         1095 t
ORIGIN      
        1 ccgcgcggat accgcgccgc tcaaggaaaa actcgaacgc gcgctaagcg ccgacgagcg
       61 cacccgttgg cgccgagaac aggaacgcgg cgagatcgac cgcacctcgc tcgcgaagct
      121 cgcgacgtcg cctggctacc gcacgccgtt tcgcacgcag cgcgcggcca aatggcgcga
      181 cgtcgccgtg accttgctga tcgatcgcag cggttcgatg gccgggcgca agatcgagct
      241 cgcgcggctg tgcgcgaccg cgctgtgcga tgcgctgacg cagttgtcat tcgactgcga
      301 ggtgctcggc tattgctcgc tcgagtccgc gccgatgaag cagctctatg agcggcaacg
      361 ggctgccggc gcggatttgg gacgctataa ccgcctcgtc gagcggctcg atctgaaggt
      421 ctacaaacgc tttggcgcga tcgatctgag cgggatcgcc gcgatcgatt gcggccacga
      481 gaatccggac ggcgaggcgc tggcctgggc cgcgacgcgg ctcgcggatc atcaggccga
      541 gcggcgcatc ctgatcgtgt tctcggacgg ctatccgtcg accggcgacg gcgatccgca
      601 ggtgttgcgc agcgatctgc gcgaacgcgt cgcggcgatc cagacacgcg gcatcgaact
      661 ggtgggcatc ggcgtcctga ccgatgcggt cgaggatttc tatccacaca acgtagtggt
      721 gagccgcctt gccgaattac cgtcgacggt attctcggtg ttgagttcca tgctgctgac
      781 gcgctaacgc gcgcgtcatg acggcatcaa acacattcga cgtagtcttc agctagcgca
      841 cgccatggat gcggcgctac accagtttag gttcgaacgg atactgcgcg atctcgccgc
      901 cccgcgcgac caccgccgtg ctttccggga tctcctgcca ccagcccggc aaatccgcga
      961 gcggctccga caacaccagg aacgcgtctt cgccggccgc cacgatgcgt gggtcgtccg
     1021 gatacagctc atgcagctgc cggaacgagc tactgtgaaa aagcgagcgc gactgccctt
     1081 cgctcgaata ccgcaccgcg acgatctgct cgccgtcggt cgcgcagacg gtcatgttca
     1141 gcggctcatc gacaccgtgt ttgcgcccga tgtcctcgac gaagcccacc attcgctcta
     1201 tcgcggtaac gggcgccagc tccaggccga aagtcagcgc gagaaaaaac agcacttcgg
     1261 aatccgtcga gccttcgatg gacggaaaca gttcaggatc gatcgccagc atcaggtcgc
     1321 gccgcacctt cggatagtcg cgcaccagcc cgttatgcgc gaagagccac cggccatgac
     1381 ggaacggatg acagttggtt tcctgcgacg gcgtgtcggt cgccgcgcgc acgtgcgcga
     1441 cgaacagcgg cgcgcggatc gcccgcgcgg cctcgcgcag attgcggtcg ctccatgcgg
     1501 gctgaatgca gcggtagcga aacggaatat cggtcggatg accgtaccag ccgatgccga
     1561 acccatcgcc gttggtggtg gtgtggccga gccgcgaatg cagactctgg tcaatcaagg
     1621 agtgtttggc gttgaacaac accgtttcga gttgaagcgg gttgccggta taagcgagcc
     1681 agcggcacat aaacttccct cctgttgcga cgcgatgtgt ggcgaacctc gcgcaacctc
     1741 gaacagccgc gcgccgccgt tcgctatttg accacgggtt gcatctgggc gatgggtatc
     1801 aggaactccg aaaaaccggt caggataatc tgcacgccga tgcacagcag caggaacgac
     1861 gagacgcgca tcgcgacctt ggtgccgtcc acgccgagat acctggcgaa aaccaccgcg
     1921 cggctataga tcagatagac ggttgccgcc accaggatcg agatcaccac cgaagcgatg
     1981 ctcgacagca cgaatgccga gagcttatgc gtgcggttcg cggtcagcgc aatggcggtg
     2041 gcgatcgaac ccggcccggt ggtgagcgga atggtgaggg ggaaaaatgc cttcgacatg
     2101 gcgttatccg aatccacgcg cttgacggcc ggctgttcgg ccggttgcgt gtccggcgca
     2161 ttgagcatct gccagccgcc gacggcgacc gcgaggcccc cgccgatccg caaggcctgc
     2221 atggaaatgc cgaagaaatg gagaatcggg gtgccgacaa agaaggcgac gagcatcacg
     2281 cacatgacgt tgaaggcgat tttcctggcg agcgcggtac ggtcttccgt ggtgagcgat
     2341 gcggtccggt cgagaaagac gaaggccatg ccgattgggt tgatgatgct gatcagcccg
     2401 gtgaagccaa acaggatatc cgagatcaga ctgtcgacca tatgcgatcc gtttcgggcg
     2461 acggctcggg cggcctgatt gactgactgc ccccggcgcg ccgcgctgag ttgaattgct
     2521 ggacgccttc gggttgtcca gagtggtgct cgcgctgcgt ggatcagatc atatcgtggc
     2581 gcggcatgca gattcgaaaa aagggtggcc acgcaagcag cgcgcggcgt tgcactgcag
     2641 cagagcggtg ccggaagatg cgcgcgcaac tttcagaaag cctacacttt tttgacttgc
     2701 tttacgcaat agaagcatcg ggcatacagt ccccagcgaa gctgcacttt caacgagacg
     2761 tacttacccc ttcgaggagt tgtccctatg ttgcttcgcg tactcgccgc gctgccgttc
     2821 gtcggcatgc tgctcggcgt tccgtttgtg aatcgtgtcg agccgctggt gctcggcatg
     2881 ccattcgtgc tggcctggat cgtcatgtgg gtggtgttga gttcgatcat catggcgatc
     2941 gtctaccgtc tggatcccac caatcgtcag ctcgccgtgg acggcgagga gggccggcca
     3001 tgagcgcact cgtcatcatt acggccatta cgctgtttgc gctgtacctg ggcgtgcgtg
     3061 cgcggcgcgg ccacgacatg aatctcgaac agtggaccgt gggcggccgc agtttcggca
     3121 ccgctttcgt gttcctgctg atggcgggcg aaatctacac gaccttcacc ttcctcggcg
     3181 gcagcggctt tgcatacggc aaaggcgcgc cggtctacta catccttgcc tacggcacgc
     3241 tggcgtacat catctcgtac tggatgctgc cgccgatctg gcgttacgcg aaacaacacc
     3301 gtctcgtttc gcagccgcac ttcttcgcgc gcaagtacga cagcccggcg ctcggcacgc
     3361 tcgtcgcgct ggtcggcgtg gctgcgttga ttccgtatct cgtgctgcaa ctcaagggcc
     3421 tcggcatcat cgtggcgacg gcctcgtatg gcgcgatttc gtcgacggcg gctgtatgga
     3481 tcggcgccgg cgtggtgacg tcctatgtga tcgtttccgg cgtgcgcggt tccgcgtgga
     3541 attcggtggt gaaggacatg ttgattctgg cgatcgtgct gttcctcggc atttatctgc
     3601 cgttgcacta ctacggcggc ttcagcgaca tgttccgggc catcgacgcc gcgcgcccgg
     3661 gcttcctgac cttcccggcc aaggggtcga gcgtgacctg gttccagtcg acggtgctcc
     3721 tgaccgcgtt gggattcttc atgtggccgc acacattcgg ttcgattttc acggcgaagg
     3781 acgagcgtat tttccggcgt aacgcgatgg tgctgccgct gtaccagttg attctgctgt
     3841 tcgtgttctt tgtcggtttt gcggcgacgc tgaaggtgcc gggcctgaag ggtggcgaca
     3901 ttgatctgtc gttgttccgc ctgtcgttgc agacgttcga cccgtggtta gtcggcgtga
     3961 ttggcgcggc ggggattctg acggcgctgg taccgggatc gatgattttg acgtcggcgt
     4021 ccacgctgct tgccaacgac gtgtatcgcg gcatggtgag ccgcaatgca tcggatgcga
     4081 cggtgaccaa actcgcgcgc tttctggtgc cggttgtggc gctggtggct gtgggtttta
     4141 cgctgcatgg tggagagacg atcgtggcat tgctgctgat ggggtacaac ttcgtgaccc
     4201 agctgtttcc ggcggtgatc tgcagtttgc tgccgaagaa tcgcgcgacc aagcaaggcg
     4261 cgttttgcgg gattcttgcg ggggttgcgg tggtggcggt gacgaccatg atgcatttga
     4321 gtattgggca gttgatgccg tttttgtctg atgctcttaa ggatgtcaat attgggtttt
     4381 tggcgcttgc tgtgaatgtg gttgtttttg ctgttgttag tgctgttacg cggccgcggg
     4441 ctgttgaggg ggctcaggct tcttcggtgc actgagggtg ggcgcctgcg gcggtggttt
     4501 ttttgttttt tgcctccgcg gcgcttattg tctgttttga ctacggcgtt gggggttcct
     4561 taagttgggt gcccggcgcc gggtttatta gctgtctgcc tatggcgttg gcctttccct
     4621 gagctgtttg cctgtgcggc gcttgttttt ctgtttgcct acggcgttgg ccttccctga
     4681 tctgtttgcc tgctgcgcgg cgctttttgt ctgtatgcct acggggttgg cctttccttg
     4741 aattgttagt ggtccattag tgttgcccct gtgcggggcg gcacttactt tctttgccgc
     4801 cgcaaagaaa gataagcaaa gaaagcggct caacccgcca gctcataagc gggtcccccg
     4861 cacagtcgcg gtagtggtgc atctggaatc tgtgttctcg cacattccgc gttggtgaca
     4921 aagggctcat acttcccgcc tcgcactacg tgcgtcgcgg atgagtctgc ctgggaaacc
     4981 atgggctccg tttgcgcttg gggggagccg ttggcttcgc ctcggcgaaa cgcacagtta
     5041 tcgtaaagac gcactccgct agcgaatg
//
LOCUS       DATASH010000004        12833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4747719,
            whole genome shotgun sequence.
ACCESSION   DATASH010000004 DATASH010000000
VERSION     DATASH010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 12833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12833
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4747719"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VHV99_00095"
     CDS             complement(<1..504)
                     /locus_tag="VHV99_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEX3633902.1"
                     /translation="MSTTPETTLAALLANRVETMPDLDVLTVEGAGVRPDEVRTYRQL
                     WENGQRLAQVLIERGLQPGEHFGLLMANHAEFVDAMVAASISGTVFVPIDPRAKGEKL
                     AFMLDNARCRGVIAADYALDNLFAVRASLPQLSWVIGLETDEGAKPLADFAGVLPYRA
                     LLPDRVPE"
     gene            complement(579..1820)
                     /locus_tag="VHV99_00100"
     CDS             complement(579..1820)
                     /locus_tag="VHV99_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX3633903.1"
                     /translation="MGNEVYIAGVGMTPFGKHLDTSVKQLTRVAVNHALADAGCDKAQ
                     LQGAFFGNSTQGHMEGQHMIRGQLALRAMGIGKIAVINVENACASASTAFHLAVNYVK
                     AGAADIVLAVGAEKMFSTDKARTFSAFDGAWDVHDTENGKRRLLDMGDGVEPPAGSIS
                     PKPYSVFMDIYASMGRMHMREFGTTQRQFAAVSAKNHGHSVHNPLAQYRQAYSIEEIL
                     GAPPITYPLTLPMCSPVSDGAAAAIVCSEAGLERLQGRRSRAIRVLASVLQTGSERPA
                     TALDRHLVRLGSLSAYEQAGVAPRDVSVAEVHDATAIGEIIQSEVLGLCEPGLGGMAA
                     ERGETTLGGRIPINPSGGLESKGHPIGATGLGQLYELVAQLRGEAGARQVEGARIAIA
                     ENGGGLCGVEEAVASITILGR"
     gene            complement(1884..2999)
                     /locus_tag="VHV99_00105"
     CDS             complement(1884..2999)
                     /locus_tag="VHV99_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5938 domain-containing protein"
                     /protein_id="HEX3633904.1"
                     /translation="MATTYEVVLYGASGYTGKLTAWKLAERGIPFIAAGRSKERLEEE
                     LAKVPELAGHDYQCVAVQHETGALTEFFKGRKVVINIVGPFMQLGRPVVEASLAAGCH
                     YFDTTGETDWMSLLKRAYGEAFAARDLALCPGNSYMWAEGNLAAEIALETPGIDTLDV
                     LYLGDSEVSVASTMSFLRMCTQPQFYLRNNELVQWPWATPYQVHVPGEHMLLNALPWS
                     GGGEPVWYQDDPRVRNCQTLVSFRNQERFLAVVGLLKKFEDECRHLDPAKQEDITNEW
                     GKAVTQTEPARENPAVNRCTLSVQGRGNTHSVSVILRGNSPYAQTGVLGAEAARRVLR
                     GQLRAPGFVSPARAFGARNLVAAQAEEGYLAYEIKSV"
     gene            complement(3051..4307)
                     /locus_tag="VHV99_00110"
     CDS             complement(3051..4307)
                     /locus_tag="VHV99_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX3633905.1"
                     /translation="MSTASDHLAAHKLGPLGAQPAPSEMSQMVQQSVHRYAQEVLRPI
                     GVKLDRMTPVEAIAPGSPYWEARDKFVGLGFGVDGLLELEPAERATTMSILFEELGWG
                     DAGLAVSYGAGLLPALMSAVSGNQFCRALAPDAKLGCWAITEPDHGSDMLDVNGMIFH
                     PQGTYGRPNCVATLRGDEVIINGQKSAWVSNGIVADVCILYCAADSGSGADTKRGVVV
                     IVPLDAKGVSRGKPLDKVGQRALNQGELFFENVTLSKKHLLAGPDQYVDAVYAIHTFA
                     NAIMATVFTGTARAAFELALAYAHERKQGGLPIIRHQSVAARLFHMFRKVEAARALAQ
                     RVVHYNFTAAQPSLHAAMTAKITATQTAFEVANDALQIFCGNGLTREYPIEKIWRDAR
                     ASLIEDGCNEVLAIKGGYYLIDPELL"
     gene            4558..7281
                     /locus_tag="VHV99_00115"
     CDS             4558..7281
                     /locus_tag="VHV99_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX3633906.1"
                     /translation="MHVIEAMPQQKPPFLLATKVFPPRLPTGLIDRPRLNGLAEQAGS
                     KRLTVIKAPAGFGKTSLALSWLARLRAGGAQLAWLSLDTEDDEPARFLHHVAQALRHA
                     CGNVGASAIGLTAEASLVPAHAVVSTLINELVDVDEEVYLFLDDYHLISLPAIHDAMS
                     FFVANAPSHVHVVLCTRTDPALPLARLRARNDLLEIDAAALRFNFEETRSFVEHECPG
                     KLRAAGVKLLFAGTEGWAAALRISTSVLSREQGKPGCELSAPSGASRPFAAYLEDMLR
                     RLPVEMVEFMLRTSILDRLTAPLCEAVTGIAASQSLLEAIAARQLLLEPMDQEGHWFR
                     YHHLMGEYLRLRLETQYRDDVPGLHLRACQWYAGQERWTDAVRHAIAAGATEDAVSLM
                     GRCAMALVTKGDLLTLLGWQRQFPAYLMRAQVTVTLAIAWGMALAMRFHDALATLDAI
                     ERDTSATNAGADAIRWESQAIRSVVAALQDDPQRALVLAQACLDQPSTDIWTTNVVSN
                     VVRFAHWKAGDLEALYATPWIPYSIEEDKRNVFSSVYRLCLLGHAEMQQMHFVLAERY
                     FTESMRLAEHHAGPQSVCVALGAPMIGHIRYEQGRLDEAEALVIDLMPVVDAAVMLDS
                     VLIAYRLLVRIAIARGDAAQAHSLLDQAQTLAYARRWDRLSAAALVERSRLYLMEGRI
                     TEAAACVAQLDRLAVSVCGPAHSVSPEIETYRALAAACVAMAQNRAQDAIESLNSALR
                     SVESRHGDYLALRLRTVLALAWLGAHERSRAIGIFREVLSVAAPAGIYRSILDQGPEI
                     GPLLQAVRDDTRSTAQTKDASAYIDLLLDGWRALYQPASKPERESLSPRERNIVELIA
                     EGQSNKEIARALGITPETVKSHVKSIFVKLAVDKRAQAVARAQALGLVGQL"
     gene            complement(7311..8612)
                     /locus_tag="VHV99_00120"
     CDS             complement(7311..8612)
                     /locus_tag="VHV99_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018435103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HEX3633907.1"
                     /translation="MSFRTDGNGNGNGSLRRASTDAPADRKAIRLKRMSRTATCLLAL
                     MIALLVACVAWQAEYPWLAWPRAFAEAGIVGAIADWYAVVALFRHPFGLPIPHTAIIP
                     RSQQRIAESLGNFVEDNFLTPALIIGRLSGHNAAHALAQWLAEPAHSRAVADALADSL
                     PGLLNSIDGADLGRFFDRVVIPQLRTLDVSRAAGGILKVLTEDDRHQPLLDRGLGALE
                     KWLTANVDLIKARFSAASRYTPAQLDAYIVNRFVEGIIALLHEVAASPDHELRRQFDA
                     AVQDLIVQLQGSAVHRRFGRSLMRDCIRHLRDGEYYRVILDHARARVAADLDREHSVL
                     REVAASLLASLGKSVRSEPAIQGKLNAWWLALARSLVVRYRRQLSALITEVVKSWDAA
                     EVGRKIEAEIGRDLQYIRINGTFVGGLIGVLLHAATLVAAR"
     gene            complement(8609..9442)
                     /gene="phaZ"
                     /locus_tag="VHV99_00125"
     CDS             complement(8609..9442)
                     /gene="phaZ"
                     /locus_tag="VHV99_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0006112 - energy reserve metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly(3-hydroxyalkanoate) depolymerase"
                     /protein_id="HEX3633908.1"
                     /translation="MTIRPESVATMQIQTVDLDGQLLRVATRHGSDASPPLLIFNGIG
                     ANLELVEPFVAALEEVSIIIFDIPGVGGSPAPVMPYRFSTLSVLSDRLLSRLGYDGPV
                     DVLGVSWGGALAQQFAHLYPGRCRRLILAATSPGVIMVPARLSVLSKLIGPRRYTDPA
                     YLQQVGAEIYGGAYRRDPALLKQHSRHIQAPRGRGYLYQLLAATGWSSLPWLGSLSQK
                     TLVMHGNDDPIVPLTNAKILAARIRDATLYVIDDGHLFLITRAKDVAPVVRKFLGQET
                     V"
     gene            complement(9537..9968)
                     /gene="phaP"
                     /locus_tag="VHV99_00130"
     CDS             complement(9537..9968)
                     /gene="phaP"
                     /locus_tag="VHV99_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068127.1"
                     /note="Members of this family are phasins (small proteins
                     associated with inclusions such as PHA granules). Note
                     that several different families of phasins have been named
                     PhaP despite very little sequence similarity to each
                     other; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01841 family phasin"
                     /protein_id="HEX3633909.1"
                     /translation="MESTNPTHLFGEYAKVIGQFKLPGIDVAALLESRRKDLDALAQA
                     NTAAFSGMQSLAQKQSEILRTTMAELQSLVTRLSASGNKPIANTGEVVQQALHKALVD
                     MQELADTAYRAQSDSFAVVTRRAAEHVEELKALLQPQQPKK"
     gene            complement(10041..11747)
                     /locus_tag="VHV99_00135"
     CDS             complement(10041..11747)
                     /locus_tag="VHV99_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX3633910.1"
                     /translation="MAVTIENQSRDTPADELAASAADGMLGPNPFVWLRPCDILATAQ
                     QIGAQALQQPALVAEQEAALARALVAVLCGSAESPPPQGDKRFGDPAWQDNAWYRLTL
                     QGYLAWRDALTGFVARSTLDQRSKERAQFVMSLLTDALAPTNTLLGNPAALKKIVESG
                     GASLIGGFRNFVMDLLKNQGMPEQVDRTAFEVGRNLANSPGAVVFRNEVLELIQYAPA
                     TEQVYGRPQLIVPPQINKFYVFDLSEGKSIVNYLVKNEFQVFAVSWRNPTAAQRDWDM
                     DTYVAALMEAIAAVRDITGSDDVNLHGACSGAMTISALLGHLASRGEKTVNAATLMVA
                     VLDSSAESQLGLFATPEAIAAAKQSSTARGVLAGEEMGRVFAWLRPNDLVWSYWVNNY
                     LLGKTPPAFDILYWNNDTTRLPAKLHGQLLDIFTGNLFSKPHALTVLGTPIDLSEVTC
                     DKYVVAGITDHITPWKGVYNSARTFGGNTRFVLSSSGHIQSLINPPGNPKAKFFVNPA
                     LPADADAWLAAAQAEKESWWSNWRDWLAARSGERRAAPAAPGNKQHRSVAQAPGTYVM
                     EA"
     gene            complement(11808..12272)
                     /locus_tag="VHV99_00140"
     CDS             complement(11808..12272)
                     /locus_tag="VHV99_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX3633911.1"
                     /translation="MSIDAYSLATIDDFVGRELGLSEWVAVDQARIDAFAQCTGDRQW
                     IHVDVERAKRESPYGGTIAHGYLTLSLLAALAIEIGLIPEDASAGLNYGLDKVRFMTP
                     VKAGARVRNRVSLVSVEKKGGGRVIVKTMNELQIEGEARPALIAETLAMLVA"
     gene            complement(12295..>12833)
                     /locus_tag="VHV99_00145"
     CDS             complement(12295..>12833)
                     /locus_tag="VHV99_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HEX3633912.1"
                     /translation="PLVDLRIVNESMEDVAHDGKAYGEVVVRAPWLTQGYLNNPEASA
                     QLWAGGYLHTQDIANIDPTGNVQITDRIKDVIKSGGEWISSLEIESLISLHPGVAEVA
                     VIGIKDDKWGERPVALVVLKADMAATEEDIKQHVLTFSASGRISKYAVPQVVKFVDAL
                     EKTSVGKMNKKWLREQFA"
BASE COUNT         2175 a         4314 c         4034 g         2310 t
ORIGIN      
        1 ctcaggcacc cgatccggca gcaacgcgcg gtatggcaac acgccggcaa aatcggcgag
       61 cggcttcgcg ccctcgtcag tttcgagccc gatcacccag gacagttgag ggagcgaggc
      121 gcgaacggca aagaggttgt cgagcgcata gtccgcggcg atgacgccgc ggcagcgcgc
      181 gttgtcgagc atgaaggcaa gcttctcgcc cttcgcgcgc ggatcgatcg gcacgaacac
      241 cgtgccggaa atcgacgcgg cgaccatcgc gtcgacgaac tccgcgtgat tggccatcag
      301 cagcccaaag tgctcgccgg gttgcaagcc ccgctcgatc aggacctgtg cgagccgttg
      361 gccgttctcc cacagttgcc ggtaagtgcg cacctcatcg ggccgcacgc cggcgccttc
      421 gacggtcagc acgtcgagat ccggcatggt ctcgacgcgg ttggcgagca acgccgccaa
      481 cgtggtttcg ggtgtcgtgc tcatggcgcc ctctctcctt gcagatgcgg ttcggtcttg
      541 cgcgtgtgcg aaatctgtcg cggtgtcacg gtccggcctc agcgtccgag aatcgtgatg
      601 ctggccacgg cctcttccac gccgcacagc ccgccgccgt tctcagcgat cgcgatgcgc
      661 gcgccctcca cctggcgggc gcctgcctca ccgcgcaact gagcgaccag ttcgtagagc
      721 tgaccgagtc ctgtcgcacc gatcggatgg cccttcgatt cgaggccgcc cgacggattg
      781 atcggaatcc gcccgcctag cgtcgtctcg ccacgttccg cggccatccc gcccaagccg
      841 ggctcgcaca gcccgagcac ttcggactgg atgatttcgc cgatcgccgt cgcgtcgtgc
      901 acctcggcga ccgagacgtc tcgcggcgcc acgcccgcct gctcataagc gctcaacgag
      961 cccagccgga ccagatgccg gtcgagcgcg gtggccggcc gttcgcttcc cgtttgcagc
     1021 accgacgcca acacacgaat ggcgcgcgag cgccgcccct gcaagcgctc gaggccggcc
     1081 tcgctgcaca cgatcgccgc ggccgcgccg tcgctgaccg gcgaacacat cggcagcgtg
     1141 agcggatagg tgatcggcgg cgcgccgaga atctcttcga tcgagtaagc ctgccggtac
     1201 tgcgcgagcg gattgtggac cgagtgtccg tggttcttgg cggacacggc ggcgaactgg
     1261 cgctgggtgg tgccgaattc gcgcatgtgc atgcggccca tcgaggcgta gatatccatg
     1321 aacacgctgt acggtttcgg cgagatgctg cctgccggcg gctcgacgcc gtcgcccatg
     1381 tcgagcagac ggcgcttgcc gttttcggtg tcatgcacgt cccacgcgcc gtcgaaggcg
     1441 ctgaaggtgc gcgccttgtc cgttgagaac atcttctcgg cgcccaccgc gagcacgatg
     1501 tcggcggcac cggctttcac gtagttcacc gcgaggtgaa acgcggtgct ggcgctcgcg
     1561 caggcgttct cgacgttgat caccgcgatc ttcccgatac ccatcgcccg cagtgcaagc
     1621 tggccgcgaa tcatgtgctg gccttccatg tgcccctgag tggagttgcc gaaaaacgcg
     1681 ccctgcaact gcgccttgtc acagcctgcg tcggcgagcg catgattcac tgctacgcgc
     1741 gtgagctgct tcacgctggt gtccagatgc ttgccgaagg gcgtcatgcc gacacccgcg
     1801 atatacactt cgttacccat atcaatctcc accgccgacc ggcgcgcacc gcgaaccgcc
     1861 ggcgcgaacc gttcgattcg ttgtcaaacg ctcttgatct cgtaggcgag gtaaccttcc
     1921 tcggcttgcg cggcgaccag attgcgcgcg ccgaacgcgc gcgccggcga cacgaaacct
     1981 ggcgcgcgca attgcccgcg cagcacgcgg cgcgccgcct ccgcaccgag cacgccggtt
     2041 tgcgcatagg gcgaattgcc acgcagaatc acgctgacgg aatgcgtatt gccccgcccc
     2101 tgaaccgaca gcgtgcagcg attcacagcc gggttttcgc gcgcgggctc agtctgagtc
     2161 acggccttgc cccactcatt ggtgatgtct tcctgtttgg cggggtcgag gtgccggcac
     2221 tcatcctcga acttcttcag cagaccgacc accgcgagaa agcgctcctg attccggaac
     2281 gacacgagcg tctgacaatt gcgcacacgc ggatcgtcct gataccagac cggctcgccg
     2341 ccgccgctcc atggcaacgc gttcagcagc atgtgctcac ccggcacatg aacctggtac
     2401 ggagtggccc acggccactg caccagttcg ttgttgcgca ggtagaactg cggctgcgtg
     2461 cacatccgca gaaacgacat cgtcgaggcg acgctgactt ccgagtcgcc gagatagagc
     2521 acgtcgagcg tgtcgatgcc aggggtctcg agcgcgatct ccgccgcgag attgccttct
     2581 gcccacatgt acgaattgcc ggggcacaac gccagatccc gggcagcgaa agcctctcca
     2641 tacgcgcgct tgagcagcga catccagtcg gtctcgccgg tggtgtcgaa gtaatggcaa
     2701 ccggccgcga ggctcgcctc gaccaccggg cgcccgagct gcatgaacgg cccgacgata
     2761 ttgatgacca ccttcctgcc cttgaagaat tcggtgagtg cgccggtttc gtgctgaacc
     2821 gccacgcact gatagtcatg tccggccagt tcgggcacct tggccagctc ctcctccagc
     2881 cgttccttgc tgcgccccgc ggcgatgaac gggataccgc gctcggcgag cttccatgcc
     2941 gtgagcttgc ccgtatagcc actcgctcca tagaggacca cttcgtaggt cgttgccatt
     3001 tcgtcgtctc cttcaggttt tcatcggatt gcgcgaccgc gccgtgccgt tcaaagcagc
     3061 tcgggatcga tcaggtagta gccgcccttg atcgcaagga cctcattgca gccgtcctcg
     3121 atcagcgacg cgcgcgcgtc gcgccagatc ttttcgatcg gatactcgcg tgtgagcccg
     3181 ttgccgcaga agatctgcag cgcgtcgttg gcgacttcga aagcggtctg ggtcgcggtg
     3241 atcttcgctg tcatcgccgc atggagcgac ggctgcgcgg ccgtgaagtt gtaatgcacg
     3301 acacgttgcg cgagcgcgcg ggcggcctcc accttgcgga acatgtggaa cagccgcgcc
     3361 gcgaccgact gatggcggat gatcggcaag ccgccctgct tgcgttcatg cgcgtaggcg
     3421 agcgcgagtt cgaacgcggc gcgcgcggtg ccggtgaaaa cagtggccat gatcgcgttg
     3481 gcgaatgtgt ggatcgcgta gaccgcgtcg acgtactggt cgggaccggc gaggaggtgc
     3541 ttcttcgaca aggtgacgtt ctcgaagaac agttcgccct ggttgagcgc gcgctggccg
     3601 accttgtcga gcggcttgcc gcgcgagacg cctttggcgt cgagcggcac gatcaccacc
     3661 acgccgcgtt tcgtgtcggc gccgctgccg ctgtccgccg cgcaatagag aatgcacacg
     3721 tcggcgacga tgccgttcga cacccaggcc gacttctgcc cgttgatgat cacctcgtcg
     3781 ccgcgcagcg tcgccacgca gttgggccgg ccgtacgtgc cttgcggatg aaagatcatg
     3841 ccgttgacgt cgagcatgtc ggagccgtga tcgggctccg tgatggccca gcagccgagc
     3901 ttcgcatccg gtgcgagtgc acggcaaaac tgattgccgg acaccgcgct catcagtgcg
     3961 ggcagcaagc cggcgccgta cgaaaccgcg agtcccgcat cgccccagcc gagctcctcg
     4021 aacaggatgc tcatcgtcgt cgcccgttcg gccggttcga gctcgagcag cccgtcgacg
     4081 ccgaaaccga gcccgacaaa cttgtcacgc gcttcccaat acggcgagcc tggtgcgatc
     4141 gcctccacgg gcgtcatgcg atcgagcttg acgccgatcg ggcgcagcac ttcttgcgcg
     4201 taacgatgca cactctgctg aaccatctgg ctcatctccg agggcgccgg ctgtgcgccg
     4261 agcgggccga gtttgtgcgc tgcaaggtga tccgatgcgg ttgacatgtc tcctccttcg
     4321 aggtggtttg gcgtatcatt tcgtgagcaa cgcaccggtc acggccggcc ccggcgagtg
     4381 gtccagcggt ggccagccag gtgcgtttca ccgttgtccg gctgactgaa tgtcgatctc
     4441 ctccatggaa tccatggtgt cccggcgcgc cgccatgcaa catcccccgt acaggggagc
     4501 gcgatggcat ggactgggga tggcagccag ggtcaacccg aatgcccgga gcaactgatg
     4561 catgtcattg aagcgatgcc gcaacagaag ccgcctttcc ttctcgccac gaaggttttt
     4621 ccgccacggc tgcccaccgg cctgatcgat cgtccccggc tgaacggtct tgccgagcag
     4681 gccgggagca aacggcttac ggtgatcaag gcgcccgccg gcttcggcaa gacttcgctc
     4741 gcgctcagct ggctcgcgcg gctgcgggcg ggcggcgctc aactggcgtg gctgtcgctc
     4801 gatactgagg atgacgaacc tgcgcgcttt ctccatcacg tggcgcaggc gttgcggcat
     4861 gcctgcggca acgtcggcgc ttccgcgatc ggcttgacgg ccgaggcctc gctcgtaccc
     4921 gcgcacgccg tcgtgtcgac cctcatcaac gaactcgtcg acgtcgacga ggaggtctat
     4981 cttttcctcg acgactatca cctgatcagc cttccggcga ttcacgacgc catgtcgttt
     5041 ttcgttgcga acgcaccttc acatgtgcat gtggtgctct gcacccgcac ggatcccgcg
     5101 ctgccgctcg cccggctgcg cgcccgcaac gatctgctgg aaatcgacgc agcagcgctg
     5161 cgcttcaact tcgaagaaac acgcagcttc gtcgaacacg aatgccctgg caaactgcgc
     5221 gccgccggcg tcaaattgct cttcgcgggc accgaaggct gggccgcggc gctgcgcatt
     5281 tccacctcgg tgctctcgcg cgagcagggc aaaccgggtt gtgaattgag cgcgccttcc
     5341 ggcgcttccc ggccgttcgc cgcgtatctg gaagacatgc tgaggcgctt gcccgtcgag
     5401 atggtcgagt tcatgctgcg cacgtcgata ctcgatcggc tgacggcgcc gctgtgcgag
     5461 gcggtcaccg gcatcgcagc gagccaaagc ctgctcgagg caatcgctgc acgccagttg
     5521 ctgctcgaac cgatggacca ggaaggacac tggttccgct atcaccacct gatgggcgag
     5581 tacctgcgcc tgcggctcga gacgcagtat cgcgacgacg tgccgggctt gcatctgcgt
     5641 gcctgccagt ggtacgccgg gcaggagcgg tggaccgacg ctgtcagaca cgccatcgcc
     5701 gcaggcgcca cggaagacgc cgtctccttg atgggccgtt gcgcgatggc gctcgtcacc
     5761 aaaggcgacc tgttgacgct gctcggctgg caacgccagt tccccgccta cctgatgcgg
     5821 gcgcaagtga cggtcacgct ggcgattgcc tggggaatgg cgcttgcgat gcgctttcac
     5881 gacgcgctcg ccacgctgga tgcgatcgaa cgcgatacct ccgccacgaa cgccggcgcc
     5941 gatgccatcc gttgggaatc ccaggcgatc cgctcggtcg tggcggcgct gcaggacgat
     6001 ccgcaacggg cgctcgtgct tgcgcaggcc tgcctcgatc aaccgtcgac cgatatctgg
     6061 accaccaatg tggtctccaa tgtcgtgcgc tttgcacact ggaaagcggg cgacctcgaa
     6121 gcgctgtacg cgacgccctg gattccctat tcgatcgagg aagacaagcg caacgtgttt
     6181 tcgtctgttt accggctctg tctgctgggc cacgcggaaa tgcagcagat gcacttcgtg
     6241 ctggccgagc ggtatttcac ggagtcgatg cggctcgccg aacatcatgc cggtccgcaa
     6301 tccgtctgtg tcgcgctggg cgcgccgatg atcggacata tccgctacga acagggccgg
     6361 ctggacgagg ccgaagcgct ggtcatcgac ctgatgccgg ttgtcgacgc cgcggtcatg
     6421 ctggatagcg tattgatcgc ctatcgtctg ctcgtgcgga tcgcgatcgc gcgcggggac
     6481 gccgcccagg cccatagcct gctcgaccag gctcagaccc tcgcttacgc gcgccggtgg
     6541 gaccggctga gcgccgccgc gctcgtcgag cgcagccgct tgtatctgat ggaaggaagg
     6601 atcacggaag ccgccgcgtg tgtcgctcaa ctcgatcggc tcgccgtctc cgtgtgcggc
     6661 ccggcgcact ccgtctcccc cgagatcgag acctaccgcg cgctggcggc ggcctgtgtg
     6721 gcgatggcgc agaatcgcgc ccaggatgcc atcgagtcgt tgaactccgc gctgcgcagc
     6781 gtcgaaagcc ggcatggcga ttacctcgcg ttacgcctgc gaaccgtgct cgcgctcgcc
     6841 tggctgggcg cgcatgaacg cagccgggcc atcgggatct ttcgtgaagt gctgagcgtt
     6901 gccgcgcccg cgggcatcta ccggtccatc ctcgatcagg gaccggaaat cggtccgttg
     6961 ctgcaggccg ttcgcgacga cacacgaagc accgcgcaaa cgaaggacgc gagcgcgtac
     7021 atcgatcttc tgctcgacgg ctggcgcgcg ctgtaccagc ccgccagcaa gcccgagcgc
     7081 gaatcgctga gcccaaggga gcgcaacatc gtcgagttga tcgccgaggg acagtcgaac
     7141 aaggaaatcg cgcgcgccct cggaattacg cctgaaaccg tcaagtctca tgtgaagagc
     7201 atcttcgtga agctggcggt cgacaagcgc gctcaggcgg ttgcgcgcgc gcaggcgttg
     7261 gggctcgttg ggcagctata ggtcgcgcgt ccctgctcgg ggcatgcgct tcagcgcgcg
     7321 gcgacaagcg tggccgcgtg gagcagaacg ccgatcagac cgccgacgaa ggtgccgttg
     7381 atccggatgt actgcaggtc gcgcccgatc tccgcctcga tcttccggcc gacttctgcc
     7441 gcatcccagc ttttcacgac ctcggtgatc agcgccgaca actggcgccg gtaccgcacc
     7501 acgagcgagc gcgcgagtgc cagccaccac gcattcagct tgccttgaat ggcaggctcg
     7561 cttcttacgc tcttgccgag cgacgccagc agactcgcgg ccacctcgcg caggaccgaa
     7621 tgctcgcgat ccaggtcggc ggccacgcgc gcacgggcgt gatccagaat cacccgatag
     7681 tattcgccgt cccgcagatg ccggatgcaa tcgcgcatca aagagcggcc gaagcggcga
     7741 tgaaccgccg agccctgcaa ctgaacgatc agatcctgca cggccgcgtc gaactggcgg
     7801 cgcagctcat gatccgggct cgcggcgact tcatgaagca gcgcaatgat gccttcgaca
     7861 aaccggttga cgatataggc atcgagctgc gcgggcgtgt agcgcgacgc cgcgctgaac
     7921 ctggccttga tcagatccac gttggccgtg agccactttt ccagcgcgcc cagtccgcga
     7981 tccagcagtg gctgatgccg gtcatcttcc gtcaggactt tgaggatgcc gcccgcggcg
     8041 cgagacacgt cgagcgtgcg cagttgcgga atcactacgc ggtcgaagaa gcgcccaagg
     8101 tccgcgccgt cgatgctgtt cagcaatccc ggcagcgagt ccgccagcgc atcggcgacg
     8161 gcgcggctgt gggccggctc ggccagccac tgggctagcg cgtgcgcggc gttatggccg
     8221 ctgagccggc cgatgatgag cgccggcgtc aggaaattgt cctcgacaaa gttgccgaga
     8281 ctctcggcga tgcgctgctg gctgcgtgga atgatcgccg tatgcggaat cggcaggcca
     8341 aacggatgcc ggaacaaggc gaccaccgca taccagtccg ctatcgcacc gactatgccg
     8401 gcctccgcga acgctcgcgg ccatgcaagc cacgggtact cggcctgcca ggcgacgcac
     8461 gcgaccagca gcgcgatcat gagcgcgagc aggcacgtcg cggttcggct catgcgcttc
     8521 aggcgaatgg ccttgcggtc cgccggtgca tcggtcgacg cgcgccgcaa gctgccattg
     8581 ccattgccat tgccgtcagt ccgaaagctc aaaccgtctc ctgcccaaga aactttcgca
     8641 ccacgggcgc aacgtctttc gcacgcgtga tcagaaacag atgcccgtcg tcgatcacat
     8701 agagcgtcgc gtcgcggata cgggccgcga gaattttcgc gttggtcaag ggcacgatag
     8761 gatcgtcgtt gccgtgcatg acgagtgttt tctggctcaa gctgccgagc cagggcaggc
     8821 tgctccagcc cgtcgcagcc agcagctgat agaggtagcc acggccccgc ggcgcctgaa
     8881 tgtggcggct atgttgcttg agcagcgcgg gatcgcgccg gtaggcgccg ccatatattt
     8941 ccgcccccac ctgctgcaga taggccggat cggtgtagcg gcgcggtccg atcagcttcg
     9001 acagcaccga gagccgcgcc ggcaccatga tgacgcctgg cgacgtggcc gccagaatca
     9061 gccggcggca gcgccccgga tacagatgcg cgaattgctg cgcgagcgcg ccgccccagg
     9121 agaccccgag cacatcgacg ggcccgtcat aaccgagccg cgacaatagc ctgtcgctca
     9181 gcacggacag cgtcgagaag cgatacggca tgaccggcgc gggcgagccg ccgaccccgg
     9241 gaatatcgaa gatgatgatg ctcacctcct ccagcgctgc cacgaacggt tccacgagtt
     9301 cgagattcgc gccaatgccg ttgaagatga gcaatggcgg cgaagcgtcg ctgccatgcc
     9361 gggtcgcaac gcgcaacagc tggccgtcca gatcgacggt ctggatctgc atggtggcga
     9421 cagactcggg gcggatagtc atttctgtca tttctctcat ccggaagacc tcatgacacg
     9481 acacgtcgga cagccgctgc actggcagca tgggtgtttc gcattgccgc gccgcgctac
     9541 ttcttcggtt gctgcggttg caggagcgcc ttcaactcct cgacgtgctc tgccgcgcgc
     9601 cgcgtcacca ccgcgaaact gtcggactgg gcacggtatg cggtatccgc gagttcctgc
     9661 atgtccacca gcgccttgtg caacgcctgc tgaaccactt cgcccgtatt cgcgatgggc
     9721 ttgttgcctg acgcgctgag ccgtgtgacg agagactgca actcagccat cgtcgtgcgc
     9781 agaatctccg actgcttttg agcgagcgac tgcatgcccg agaacgcggc cgtgttggct
     9841 tgcgcgagcg cgtcgaggtc cttgcggcgc gactccagaa gcgcggcgac gtcgatgccc
     9901 ggcagcttga actgcccgat tacttttgcg tattcaccga aaagatgggt gggattggtt
     9961 gattccatga tcaagactcc ataagaactg cattgaaaaa atcctcggcc atgacgcgat
    10021 gacacggcca ctggggtgcg tcatgcttcc atcacgtaag tgccgggcgc ctgcgcgacc
    10081 gaccgatgct gcttattgcc cggcgctgcc ggcgcggccc ggcgttcacc cgaacgggcg
    10141 gccagccagt ctcgccagtt gctccaccac gattcctttt cggcctgtgc tgcggcgagc
    10201 cacgcgtcgg catcggccgg cagcgccgga ttgacgaaga acttcgcctt cggattgccc
    10261 ggcggattga tcaggctctg gatatggccg ctcgaactca gcacgaagcg cgtattgccg
    10321 ccgaaggtcc gcgccgagtt gtagacgccc ttccacggcg tgatgtgatc cgtgatgccg
    10381 gccacgacgt acttgtcgca cgtcacctcg gacagatcga tcggcgtacc cagcaccgtc
    10441 agcgcatgcg gcttgctgaa caggttgccc gtaaagatgt cgagcagttg tccatgcagc
    10501 ttcgcgggca gccgggtcgt gtcgttgttc caatagagga tgtcgaaagc cggcggcgtc
    10561 ttgcccaaca gatagttgtt gacccagtag ctccacacca gatcgttggg gcgcagccac
    10621 gcgaacacgc ggcccatctc ctcgccggcg agcacgccgc gcgcggtgct gctctgcttg
    10681 gcggccgcga tcgcctccgg cgtcgcgaac agaccgagct gcgactccgc gctgctatcc
    10741 agcacggcga ccatcagcgt ggcggcgttg acggtctttt caccgcggct cgcgagatgc
    10801 ccgagcagcg ccgagatcgt catcgcgccg gagcacgcgc cgtgcaggtt cacgtcgtcg
    10861 ctgccggtga tgtcgcgaac cgccgcgatc gcttccatca acgcggcgac gtaggtatcc
    10921 atgtcccagt cacgctgcgc ggcggtgggg ttgcgccagc tcacggcgaa cacctggaac
    10981 tcgttcttca ccagatagtt gacgatgctc ttgccttccg acaggtcgaa cacatagaac
    11041 ttgttgatct gcggcggcac gatcagttgc gggcggccgt acacctgctc cgtagccggt
    11101 gcgtactgga tcagctcgag cacctcgttg cgaaacacca ccgcgcccgg cgagttggcg
    11161 agattcctgc cgacttcgaa cgccgtgcgg tccacctgct cgggcatgcc ctgattcttc
    11221 agcaggtcca tcacgaagtt gcggaacccg ccaatcaggc tcgcgccgcc cgactcgacg
    11281 atcttcttga gcgccgccgg attgccgagc agcgtattgg tcggcgccaa cgcatcggtc
    11341 aggagcgaca tcacaaactg cgcgcgctcc ttgctcctct ggtccagcgt cgaacgggcg
    11401 acgaagccgg tgagcgcgtc gcgccacgcc agatagcctt gcagcgtcaa gcggtaccac
    11461 gcgttgtcct gccacgcagg atcgccgaag cgcttgtcgc cctgcggcgg cggcgattcc
    11521 gcgctgccgc acagcacggc aaccagcgcc cgggcgagcg ccgcttcctg ttccgcgacg
    11581 agcgcgggtt gctgcaacgc ctgggcaccg atctgctgtg cggtggcgag gatgtcgcac
    11641 ggacgcagcc agacaaacgg gttgggacca agcatgccgt ccgcggcaga cgccgcgagc
    11701 tcatcggccg gggtgtcccg cgattgattt tcaatggtta ccgccatttt gctgctcctc
    11761 aagcgaccgc ctggtcgccg tcaattcgtc ttcgtcaggc ggccggctca cgccaccagc
    11821 attgccagcg tctccgcgat cagggccggc ctggcctcac cctcgatctg caactcattc
    11881 atcgtcttga cgatgacgcg gccgccgccc tttttctcca ccgacaccag gctcacgcgg
    11941 ttgcgtaccc gtgcgccggc cttgaccggc gtcatgaagc gcaccttgtc gagcccgtag
    12001 ttcaggccgg ccgaggcgtc ttcgggaatc aggccgatct cgatcgcgag tgcggccagc
    12061 aaggacaacg tcagatagcc atgcgcgatc gtgccgccgt aggggctctc gcgcttcgcg
    12121 cgctccacgt ccacgtgaat ccactgccgg tcgccggtgc attgcgcgaa tgcatcgatg
    12181 cgcgcctggt cgaccgccac ccactccgac agacccagct cgcggccgac gaagtcgtcg
    12241 atcgtggcaa ggctgtatgc gtcgatactc atcgtgtctg ctcctcgtga ccggtcaggc
    12301 gaactgttcg cgcaaccact tcttgttcat cttgccgacg ctcgtcttct ccagcgcgtc
    12361 gacgaacttg acgacctgcg gcaccgcata cttcgaaatc cgccccgacg cgctgaacgt
    12421 cagcacgtgc tgtttgatgt cttcctcggt ggccgccata tcggccttca acaccaccag
    12481 cgccacgggc cgttcgcccc acttgtcgtc cttgatgccg atcaccgcga cttcggcgac
    12541 acccggatgc aacgaaatca ggctttcgat ttcaagcgac gagatccact cgccgcccga
    12601 cttgatcacg tccttgatcc ggtccgtgat ctgcacgttg ccggtcgggt cgatattggc
    12661 gatgtcctgg gtatgcagat agccgccggc ccagagctgc gcggaggcct cgggattgtt
    12721 cagatagcct tgtgtgagcc acggcgcgcg cacgacgacc tcgccgtatg ccttgccgtc
    12781 gtgcgcaacg tcctccatgc tttcgttgac gatgcgcagg tcgacgagcg gga
//
LOCUS       DATASH010000005         5645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_593913,
            whole genome shotgun sequence.
ACCESSION   DATASH010000005 DATASH010000000
VERSION     DATASH010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5645
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593913"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            271..2622
                     /locus_tag="VHV99_00150"
     CDS             271..2622
                     /locus_tag="VHV99_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX3633913.1"
                     /translation="MPDLLANLNPEQHAAVTLPNEPALILAGAGSGKTRVLITRIAWL
                     IQHGLGSPATILAVTFTNKAAREMMSRLSALLPIDTRGMWIGTFHGLCNRMLRAHHRD
                     AGLPATFQILDTADQLSAIKRLMKGLNIDDEKYPAKNLQYFINNSKEQGLRPKDVDAT
                     DNFNRKFVELYEAYDQQCQREGVVDFPELLLRCFELLAHNPPLRAHYQARFKHILVDE
                     FQDTNKLQYAWLKMLAGQTNSIFAVGDDDQSIYAFRGANVGNMRDFEHEFNVRHMIKL
                     EQNYRSHGHILDAANQLIANNSRRLGKNLRTDAGHGEPVRVYESATDTQEAGWIVEEI
                     KALISTGTSRSEIAILYRSNAQSRTIEHTLVNAGIAYRVYGGLRFFERQEVKHALAYL
                     RLIDNPNDDTAFARVVNFPTRGIGARSIEQLADAARLYNCSMAAAIPYVAGKAGSSLA
                     SFANLIGKMRAETQQMSLPETVEYVVRTSGLTEFYQNEREGQDRLENLQELVNAAAAF
                     VSEEGYGMDTPARSIPLRPGATAAPELAVATDDPNVVVLDAPGIADPAQNPDTMTPLA
                     GFLSHASLEAGDNQAQAGQEAVQLMTVHAAKGLEFTAVFITGLEEGLFPHENSAMESD
                     GLEEERRLMYVAITRAKERLYLSFAQSRMLHGQTRYNIRSRFFDELPQETLKWLTPKV
                     EAGARWGGRSDNAGYGRDWFARPGYTGAASSTPAPLPAFANEQRAAEAGFRIGQQVFH
                     TKFGEGTITALEGGGTDAKAQVKFKRHGEKWLALAVAKLQAVE"
     gene            2619..3518
                     /locus_tag="VHV99_00155"
     CDS             2619..3518
                     /locus_tag="VHV99_00155"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEX3633914.1"
                     /translation="MSANAIGVAAAGASTVDVGAAGRSQTAVSTAGSSAPGAAPAHRG
                     TPVSHRPLGILAALPQELGDLIEAMRAESGVRTITHGQRDYHLGTVHGAPCVVTLARV
                     GKVAAAATVSALIHAFDVEAVVFTGVAGGVGTEVRVGDIVVANVLMQHDLDASPLFPR
                     FEVPLLGVSRFAADAALADRLAAACERFVAEEGALQAARFGTREPRVHRGLIISGDQF
                     VASATGVKALRDALPDALAVEMEGAAIAQVCYEYGVPCAVVRTISDTADDHAPESFVS
                     FLTEIAGTYSNAILTRFLEARRA"
     gene            complement(3631..5535)
                     /locus_tag="VHV99_00160"
     CDS             complement(3631..5535)
                     /locus_tag="VHV99_00160"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HEX3633915.1"
                     /translation="MTRYRDFHRRSIEQPEAFWREQAQRIHWQTPFDTVLDRSNPPFA
                     RWFVGGRTNLCHNAVDRHLVERAQQNALVYVSTETGIERRYTYAELYAEINRMAAVMR
                     SLGVKRGDVVLLYLPMIPEALFAMLACARLGAIHSVVFGGFAAPNLAARIDDAKPVLI
                     VTADAGARGGKVIDYTPLVDEALARAAHKTPHVLLIDRQLAPERLNANYLVAYEPLRE
                     QFFDAHVPCEWLESNEPSYVLYTSGTTGKPKGVQRDVGGYAVALAASMEHIFQGKPGD
                     TMFTASDVGWVVGHSYIVYAPLIAGLTTVMYEGTPIRPDGGIWWHLVEQHKINLMFTA
                     PTALRVLKKQDPALLKKADLSSLRTLFLAGEPLDEPTATWIADALGKPVIDNYWQTET
                     GWPMLAIPRGVETLPTRLGSPGVPSAGFNLSLRNELTGEPCPPGEKGVLTLAYPLPPG
                     CMSTVWGDDSRFISTYWSSIPNQRVYSTFDWGIQDKNGYVTILGRTDDVINVAGHRLG
                     TREIEEALSSHAAVAEVAVVGVTDALKGQAAMAFVVLRDAQAYANDKARGKLEAELTA
                     TVDRQLGAIARPSRVVVVSMLPKTRSGKLLRRAIAALAEGREPGDLPTIEDPVALQQV
                     REALEDSTKH"
BASE COUNT         1021 a         1838 c         1811 g          975 t
ORIGIN      
        1 accactaccg tcactgtgcg tgggactcgc ttaagaacta gcggtgtgag ccgctttctt
       61 ttgcctactt gtctttgcgg cacggcaggc aaagaaaagt aggttcttgc cccgcacagg
      121 ggcgacgcta atagaccaat aacaaagcaa ggaaaggccc acgccgtagg catacagaca
      181 aacaagcgct acgcaggcaa aacaaagcca ataaagggcc acccccgcac aagcatataa
      241 tgtcgaacgc actcgccgtc ctcccaaaaa atgcccgatc tgctagccaa tctaaacccc
      301 gaacaacacg ccgccgtcac gctcccgaac gagccagcgc tcatccttgc cggcgcgggc
      361 agcggcaaaa cccgcgtcct catcacgcgg atcgcatggc tcatccagca cggtctgggc
      421 tcgcccgcca ccatcctcgc ggtgaccttc accaataaag ccgcccgcga aatgatgtcg
      481 cgtctttcgg cactcttgcc gatcgatacg cgcggcatgt ggatcggcac cttccacggc
      541 ctgtgcaacc gcatgctccg cgcgcatcat cgcgacgccg gcctgccggc cacgttccag
      601 atcctcgaca ccgcggacca gctctccgcg atcaagcgtc tgatgaaggg cctcaatatc
      661 gacgatgaaa agtacccggc caaaaatctc cagtacttca tcaacaattc gaaagaacaa
      721 ggcctgcgcc cgaaagacgt cgacgccacg gacaacttca accgcaaatt cgtcgaactc
      781 tacgaagcct acgaccagca atgccagcgc gaaggcgtcg tcgacttccc ggaattgctg
      841 ctgcgctgct tcgaactgct cgcgcataat ccgccgctgc gcgcccacta tcaggcgcgc
      901 ttcaagcaca tcctcgtcga cgagtttcag gacaccaaca agctgcaata cgcgtggctg
      961 aagatgctgg ccgggcaaac caactcgatc ttcgcggtcg gcgacgacga ccagtcgatc
     1021 tacgcattcc gcggcgcaaa tgtcggcaac atgcgtgact tcgagcatga gttcaatgtc
     1081 cggcacatga tcaagcttga gcagaactac cgctctcacg gccatatcct cgacgcggcg
     1141 aatcaactga tcgccaataa ctcgcggcgt ctgggcaaga atctgcggac cgacgcgggt
     1201 cacggcgaac cggtgcgcgt ctacgagtcg gccaccgata cgcaggaagc cggctggatc
     1261 gtcgaggaaa tcaaggcgct gatcagtacc ggcacgtcgc gcagtgaaat cgccatcctg
     1321 tatcgcagca acgcgcagtc gcgcacgatc gaacacacgc tggtcaatgc gggcatcgcc
     1381 tatcgcgtgt acggcggttt gcgctttttc gagcgtcagg aagtcaagca cgcgctcgcc
     1441 tatctgcgcc tgatcgacaa tccgaacgac gacacggcgt tcgcccgcgt cgtcaatttc
     1501 ccgacccgcg gcatcggcgc gcgctcgatc gagcaacttg ctgatgcggc gcggctgtac
     1561 aattgttcga tggctgcggc gattccgtat gtcgcgggca aggcggggtc gagcctcgcc
     1621 agcttcgcga acctgatcgg caagatgcgc gcggaaaccc agcagatgag cctgccggaa
     1681 acggtcgaat acgtggtccg cacgagcggt ctcacggagt tctatcagaa cgaacgcgaa
     1741 ggccaggacc ggctcgagaa cttgcaggaa ctggtcaacg cggctgccgc tttcgtcagc
     1801 gaggaaggct acggcatgga tacgccggcg cgctcgattc cgctgcgccc gggcgcgacc
     1861 gcggctcccg agttggcggt cgccaccgac gatccgaatg tcgtcgtgct ggacgcgccc
     1921 ggcatcgccg atccggcaca aaacccggac acgatgacgc cgctcgccgg tttcctgtcg
     1981 cacgcatcgc tggaagcggg tgacaaccag gcgcaagccg gtcaggaagc cgtccagttg
     2041 atgaccgtgc acgcggccaa gggcctcgag ttcacggcgg tgttcatcac cgggctcgaa
     2101 gaagggctgt tcccgcatga aaacagcgcg atggaatcgg acgggctgga ggaagagcgt
     2161 cgtctgatgt acgtggcgat cacgcgggcg aaggagcgtc tgtatctgtc gttcgcgcag
     2221 agccggatgc tgcacggcca gacgcgctac aacatccgtt cacgcttctt cgacgaactg
     2281 ccgcaggaaa ccctcaagtg gctcacgccg aaggtcgagg ccggggcgcg ctggggtggc
     2341 cgctcggata acgccggcta tggccgcgac tggttcgcgc ggccgggtta cacgggcgct
     2401 gcgtcgtcga caccggcgcc gttgccggcc tttgcaaacg aacagcgcgc ggctgaagcg
     2461 ggttttcgca tcggccagca ggtgttccac accaagttcg gggaaggcac gatcaccgcg
     2521 ctcgaaggtg gcggcacgga tgcgaaggcc caggtcaaat tcaagcggca cggcgagaag
     2581 tggctggcgc tggcggttgc caaattgcag gcggtcgaat gagcgcgaac gcaattggcg
     2641 tagcggcggc tggtgcaagc acggtcgacg tgggtgctgc gggtcgaagc cagactgccg
     2701 tcagtactgc gggttctagc gcgccgggcg ccgctccggc gcaccgtggc acgcccgtct
     2761 cgcatcgtcc gctcggcatt ctcgcggcct tgccgcagga actcggcgat ctgatcgaag
     2821 caatgcgcgc cgaatccggc gtgcgcacca tcacgcacgg tcagcgtgac tatcacctcg
     2881 ggactgtgca cggcgcgcct tgcgtcgtga cgttggcgcg ggtcggcaag gtcgcggcgg
     2941 cggccactgt cagcgccctg atccatgcgt tcgatgtcga ggcggtcgtg tttaccggcg
     3001 tcgcgggtgg cgtgggaacg gaggtgcggg ttggcgatat cgtcgtcgcc aacgtgctga
     3061 tgcagcacga tctcgacgcg tcgccgctct ttccgcggtt tgaagtgccc ttgctcggcg
     3121 tgtcgcgctt tgcagccgat gcggcgctcg ccgatcggct cgccgcagcg tgtgagcgtt
     3181 ttgttgccga agagggcgcg ctgcaggcgg cgcgcttcgg cacacgcgag ccgcgcgtgc
     3241 atcgcggctt gatcatcagc ggcgatcagt tcgtcgcgag tgcgacgggc gtcaaggcgc
     3301 tacgcgatgc gctgccggat gcgctcgcgg tcgagatgga aggcgcggcg atcgcgcagg
     3361 tctgttacga gtacggcgtg ccgtgtgcag tggtgcgcac catttccgat accgcggacg
     3421 atcacgcgcc cgagtcgttc gtatcgtttc tcacggagat cgccgggacg tattcgaacg
     3481 cgatcctgac gcggtttctc gaggcgcgtc gcgcgtgatc tgctcgccgc ttcggcgtgc
     3541 cgcgtgttga tcggccggtc cactagaagt agctcgactg gctggcgcag gtgtgctgct
     3601 cgttcacggc atgggcgggc cgggcgccgg ctagtgtttc gttgaatcct ccagtgcctc
     3661 gcgcacctgc tgcaacgcca ccggatcttc gatcgtcggc aaatcgcccg gctcgcgtcc
     3721 ctcggccagc gccgcgatcg cgcggcgcag cagcttgccg gagcgcgtct tcggcagcat
     3781 cgacaccacc accacgcgcg agggccgcgc aatcgcgccg agctggcggt cgaccgtcgc
     3841 ggtgagttcg gcctccagct tgccgcgcgc cttgtcgttc gcgtaagcct gcgcgtcgcg
     3901 cagcactacg aaggccatcg ccgcctgacc cttcagcgca tccgtcacgc cgaccaccgc
     3961 cacttccgcg accgccgcgt ggctcgacaa cgcttcctcg atctcgcgcg tgccgagccg
     4021 atggcccgcg acgttgatca cgtcgtcggt gcggccgagg atcgtgacgt agccgttttt
     4081 gtcctgaatg ccccagtcga acgtcgagta gacccgctgg ttcggaatgc tcgaccagta
     4141 ggtgctgata aagcggctgt cgtcgcccca taccgtcgac atgcagcccg gcggcagcgg
     4201 ataagccagc gtgagcacgc ctttttcgcc aggcgggcag ggctcgccgg tcagttcgtt
     4261 gcgcaagctc agattgaagc cggccgacgg cacgcccggc gaaccgagcc tggtagggag
     4321 cgtctcgacg ccgcgcggaa tcgccagcat cggccagccg gtttcggtct gccagtagtt
     4381 gtcgatcacg ggcttgccga gcgcgtcggc gatccatgtc gcggtcggtt cgtcgagcgg
     4441 ctcgccggcg aggaacagcg tgcgcaggct cgacaggtcg gctttcttca acagcgcggg
     4501 gtcctgcttt ttcagcacgc gcaacgcggt cggcgcggtg aacatcagat tgatcttgtg
     4561 ctgctcgacg aggtgccacc agatgccgcc gtccggccgg atcggcgtgc cctcgtacat
     4621 cacggtagtc agccccgcga tcagcggcgc atagacgata tagctatggc cgaccaccca
     4681 gccgacgtcc gacgcggtga acatcgtgtc gcccggcttg ccctgaaaga tgtgttccat
     4741 cgatgccgcc agcgccaccg catagccgcc gacgtcgcgc tgcacgccct tcggcttgcc
     4801 ggtcgtgccc gacgtgtaca gcacatacga aggctcgttc gattcgagcc attcgcatgg
     4861 cacatgggcg tcgaaaaact gctcgcgcag cggttcgtag gcgacgaggt agttcgcgtt
     4921 caggcgctcg ggcgcgagct ggcgatcgat cagcaggaca tgcggcgtct tgtgggccgc
     4981 gcgggcgagc gcttcgtcca ccagcggcgt gtagtcgatc accttgccgc cgcgtgcgcc
     5041 ggcgtcggcg gtcacgatca gcacaggttt cgcatcgtcg atgcgggccg ccagattggg
     5101 ggccgcgaag ccgccgaaca ccaccgagtg aatcgcgccg agccgtgcgc acgcgagcat
     5161 cgcgaacagc gcttcgggga tcatcggcag atagagcagc acgacgtcgc cgcgcttcac
     5221 acccaacgag cgcatcaccg cggccatccg gttgatttcc gcgtagagct cggcgtaggt
     5281 gtaacgccgc tcgatgcccg tttccgtcga cacatacacc agcgcgttct gctgtgcgcg
     5341 ctcgaccaga tggcgatcca ctgcgttatg gcacaggttt gtccggccgc cgacgaacca
     5401 gcgcgcgaac ggcggattcg agcggtcgag cactgtatcg aacggcgtct gccagtgaat
     5461 ccgctgcgcc tgttcgcgcc agaacgcctc gggttgctcg atcgaacggc ggtggaagtc
     5521 gcggtaacgg gtcatctgcg gcgatccttc tgctgcgtga gtgaacgggt cgagtcgtgc
     5581 ggctgttgcg cgcggcggga aagcagtgcg tatgtccgca caagcataga gcacccgcgg
     5641 cgctg
//
LOCUS       DATASH010000006         5562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6923985,
            whole genome shotgun sequence.
ACCESSION   DATASH010000006 DATASH010000000
VERSION     DATASH010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5562
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6923985"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..1046
                     /locus_tag="VHV99_00165"
     CDS             75..1046
                     /locus_tag="VHV99_00165"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEX3633916.1"
                     /translation="MKTTFLDFEQPIAELEGKIEELRFVQDDSAVDISEEIERLSKKS
                     QQLTKDLYANLTPWQVSQIARHPQRPYTFDYVSELFTDFHELHGDRNYADDLSIVGGL
                     ARFNGQACMVIGHQKGRDTKERALRNFGMPRPEGYRKAERLMRLAEKFSLPIFTFIDT
                     PGAYPGIGAEERGQSEAIGRNLYVMAELKTPLIATIIGEGGSGGALAIAVGDSVLMLQ
                     FSTYSVISPEGCASILWKSAAKAPEAAEALGLTAHRLKALGLIDKIVNEPLGGAHRDP
                     KSMAAMLRRALSDSLRQFQGMSINDLRQRRFERLMSYGKFKETTPGA"
     gene            1095..2639
                     /gene="tilS"
                     /locus_tag="VHV99_00170"
     CDS             1095..2639
                     /gene="tilS"
                     /locus_tag="VHV99_00170"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEX3633917.1"
                     /translation="MTPTADSPADRLVLEAVGVALSALPASARIAVAFSGGVDSSVLL
                     DATVRVAGASRCVALHVHHGLSAHADEWLAHCEAFARERGVEFAAQRVEVSRDASVSV
                     EAAARDARYRALDAMCATRGVHTLWLAQHADDQAETVLLQLLRGAGLAGLAAMAPEYL
                     PAGAAALRVRPLLHLLRAQLEQYASARNLHWIDDESNADTRYARNALRHEVTPALAVH
                     FPGFRDALARTAAHAASAQRLLDDLARIDLDGAVSDAGRALSHDALLALDDDRALNLL
                     RYWMRTLGLVAASTARLVDALRQLREVGAAGEGHSLRIDHAGQALRSYRGLVYWEAGD
                     SSEAADETALSTREVSELAWQGEVIWRLPQWRGTFVFIDADTNRDGGADADADAVADA
                     DADADSDGDGDRDGEAPGNAASDDPNTLPLSVLRRALLSARSRSGGERMRTSAAPGSP
                     RRTLKNLFQERGVPAWKRDVPLLYVGDELLFVPLLGVNRAALPEQVDAAEPRIRIIWR
                     EDLLVA"
     gene            3024..4274
                     /locus_tag="VHV99_00175"
     CDS             3024..4274
                     /locus_tag="VHV99_00175"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006408212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX3633918.1"
                     /translation="MALIVHKYGGTSMGSVERIKNVAKRVAKWHKAGHKMVVVPSAMS
                     GETNRLLGLAKEITGQPSPRELDMIAATGEQVSSGLLAIALQEAGVEAVSYAGWQVPV
                     KTDSAFTKARISEIDGERVLRDLDAGKVVVITGFQGIDPDGHITTLGRGGSDTSAVAV
                     AAALKADECLIYTDVDGVYTTDPRVVEEARRLDRVTFEEMLEMASLGSKVLQIRSVEF
                     AGKYQVKTRVLSSLTDPLIPLDTEMKSGTLITFEEDETMEKAVISGIAFQRDEARIAV
                     MGVPDKPGIAYQILGPVADANIDVDMIIQNQSVDGKTAFTFTVGRGDYQRAMDILTGQ
                     VKGHVQAEQVLGDPKVSKVSVVGVGMRSHVGIASTMFRTLSEEGINIQMISTSEIKIS
                     VLIDEKYMELAVRALHKAFELDQA"
     gene            4548..4641
                     /locus_tag="VHV99_00180"
     tRNA            4548..4641
                     /locus_tag="VHV99_00180"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4582..4584,aa:Ser,seq:gct)
     gene            5008..5355
                     /locus_tag="VHV99_00185"
     CDS             5008..5355
                     /locus_tag="VHV99_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3633919.1"
                     /translation="MKSARLVPLLFGVLGLAASGAAMAGGLNVGVNIGIPAPVYVAPA
                     PVYAPPPPPPPSVVYQPAPVYYGGSAIVIGWHGDRYWDGRRYWARDDWYRHHPPGRYD
                     YGRNHYDDHRGWH"
     gene            complement(5476..>5562)
                     /locus_tag="VHV99_00190"
     CDS             complement(5476..>5562)
                     /locus_tag="VHV99_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006026016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabI"
                     /protein_id="HEX3633920.1"
                     /translation="LAAGVTAEIVHVDSGFNAVVGGMVAVAE"
BASE COUNT         1048 a         1674 c         1756 g         1084 t
ORIGIN      
        1 agttctaccc cgattttgac attggccggt gcggttagaa tacgcgctgc cgccaagagc
       61 aaggaatgct agacatgaag accacctttc tggatttcga acagccgatc gcggagctcg
      121 aaggcaagat cgaagagctg cgtttcgttc aggacgattc ggccgtcgac atttcggaag
      181 agatcgagcg gctgtcgaag aaaagccagc aactcacgaa agatctgtac gcgaacctga
      241 caccgtggca ggtatcgcag atcgcgcgcc atccgcagcg gccctacacg ttcgattacg
      301 tgagcgagct gttcaccgat ttccacgaac tgcacggcga ccgcaactat gcggacgacc
      361 tgtcgatcgt cggcggcctt gcgcgtttca atggccaggc ctgcatggtg atcggccatc
      421 agaagggccg cgacaccaag gagcgcgcgc tgcgcaactt cggcatgccg cgtccggaag
      481 gctatcgcaa ggccgaacgc ctgatgcgtc tcgccgagaa gttcagcttg ccgatcttca
      541 cgtttatcga tacgccgggc gcttacccgg gcatcggcgc ggaagagcgt ggccagtccg
      601 aggcgatcgg ccgcaatctg tatgtgatgg ctgaactcaa gacaccgctg atcgccacga
      661 tcatcggcga aggcgggtcg ggcggcgcgt tggcgattgc tgtcggcgac agcgtgctga
      721 tgctgcaatt ctcgacctat tcggtgattt cgccggaagg ctgcgcatcg atcctgtgga
      781 agagcgccgc gaaagcgccg gaagcagcag aggcgctcgg cctgaccgcg catcgtctga
      841 aggcgctcgg cctgatcgac aagatcgtga acgagccgct cggcggcgcg catcgcgatc
      901 cgaagagcat ggccgcaatg ctgcgtcgtg cgctctccga ttcgctgcgc cagttccagg
      961 gcatgagcat caatgacctg cgtcaacgcc gtttcgaacg gttgatgtcg tacggcaagt
     1021 tcaaggaaac gacgccgggt gcctaagccc gcgcgcgttg tctttcgtct tccagcgctt
     1081 cacgcgctag cgacgtgact cccaccgccg attcgcccgc cgaccgcctc gttctcgaag
     1141 cggtcggcgt ggcgctgtcc gcgcttcctg cgagtgcgcg cattgcggtt gcgttcagtg
     1201 gcggcgtcga ttcaagtgtg ctgctcgatg caacggtgcg tgtcgcgggc gcgtcgcgtt
     1261 gtgtcgcgct gcatgtgcat cacggcctca gtgcccacgc cgacgaatgg ctcgcgcact
     1321 gcgaagcgtt tgcgcgggaa cgtggcgtcg aattcgcggc acagcgggtc gaggtgtctc
     1381 gtgacgcgag cgtgagcgtc gaggccgcgg cacgcgatgc gcgttatcga gcgctggacg
     1441 cgatgtgcgc aacccgcggc gttcacacgc tatggctcgc gcaacatgcg gacgatcagg
     1501 cggagaccgt gctgttgcag ttgttgcgcg gcgcgggcct cgcgggtctt gcggcgatgg
     1561 cgcccgagta cttgccggcg ggtgccgctg cgctgcgcgt gcgacctttg ttgcacctgc
     1621 tgcgggcgca actcgagcag tatgcgagcg cccgcaattt gcactggatc gacgacgaat
     1681 cgaacgcgga cacacgttat gcgcgcaacg cattgcgcca cgaggtgaca ccggcgctgg
     1741 ccgtccactt tcctggtttt cgcgacgcac tcgcgcgcac ggcggcgcat gcggcatcgg
     1801 cgcaacgtct gcttgacgat ctcgcgcgca tcgatcttga cggagcagtg agcgatgcag
     1861 gccgtgcgct atcgcatgac gcgttgctcg ccctcgacga tgatcgtgcg ctcaacctgc
     1921 tgcgctactg gatgcgcacg ctgggtcttg ttgcggcatc aaccgcgcgt ctggtcgacg
     1981 ctttgcgcca gttgcgcgaa gtcggcgcgg ctggtgaggg ccatagcctg cggatcgatc
     2041 acgcggggca ggcgttgcgc agctatcgcg gcctcgtcta ttgggaagcg ggcgatagta
     2101 gcgaggccgc cgacgaaacc gcgctctcca cgcgcgaggt cagcgaactc gcatggcagg
     2161 gtgaagtgat ctggcgtttg ccgcagtggc ggggcacctt cgtgtttatc gatgccgata
     2221 cgaatcggga tgggggtgcc gatgccgatg ccgatgccgt tgccgatgct gatgccgatg
     2281 ctgatagtga cggtgacggt gatcgtgatg gcgaggcgcc gggcaacgca gcgtccgacg
     2341 atcccaatac gcttcccctc agcgttctac gccgggcact gttgagcgcg cgctcacgca
     2401 gcggcggcga acggatgcgc acgagcgccg caccaggcag tccacgccgc acgctaaaaa
     2461 acctgttcca ggagcgtggc gttcccgcat ggaaacgcga cgtgccgctg ctctacgtcg
     2521 gtgacgaact gctgttcgtg cccttgctcg gtgtgaaccg cgccgcgttg cccgagcagg
     2581 tggatgcggc cgaaccgcgt atccggatca tctggcgcga ggatttgctg gtcgcctgac
     2641 cgaccggtcg gccatcggcg taatcgatcg agggaatccg taaatagcgc gcaaaaccgc
     2701 accggacaag cctttcgagc gcgtaatagg gaccattcga cggccgtttt ggcttgtttt
     2761 tttacgctcg atcgggtaat ttagcttgtt tgcccgaccc gatttcgccg tcgcttttgt
     2821 caattttgtc ggtttgcgat cgtgatccgt cgtttccggt aagcctctag cgatcattcc
     2881 cgggcaaatc cgcgcgtgct gcacgcgtgc tgcatctacg ccgccacaca ctttgccgcc
     2941 gttcacaaga cgctgcgctc ctgtgctttt gtgcggtcgt ctccagcgcg ccagcgcggt
     3001 ttatcctcca gttcagaacg acaatggcac tcatcgtaca taaatacggc ggcacctcga
     3061 tgggctcggt cgagcgcatc aagaacgtcg ccaagcgcgt cgcgaaatgg cacaaggccg
     3121 gccacaagat ggtcgtcgtg ccgtcggcaa tgtcaggcga aaccaaccgt ttgctcggtc
     3181 tcgcgaaaga aatcacgggt cagccgagcc cgcgcgaact cgacatgatc gccgccaccg
     3241 gcgagcaggt cagctcgggt ctgctcgcca ttgcgctgca ggaagccggt gtcgaggcgg
     3301 tcagctatgc cggctggcaa gtgccggtca aaacggatag cgcgttcacg aaagcgcgca
     3361 ttagcgaaat cgacggcgaa cgcgtgctgc gcgatctcga cgccggcaag gtggtcgtga
     3421 tcacgggctt ccagggtatc gaccctgacg gccacatcac cacgctcggc cgtggcggtt
     3481 cggatacgtc cgctgtcgca gtcgctgccg cgttgaaagc cgacgagtgc ctgatttaca
     3541 cggacgtcga cggcgtctac acgaccgacc cgcgcgtggt ggaggaggcg cgccgcctcg
     3601 atcgcgtgac gttcgaagaa atgctggaaa tggcaagcct gggttccaag gtgctgcaga
     3661 tccgctcggt ggaattcgcc ggcaaatatc aggtaaaaac gcgtgtgctg tcgagcctga
     3721 ccgatccgct catcccgctc gacactgaaa tgaagtcggg caccctgatt acttttgaag
     3781 aagacgagac catggaaaaa gcagtcatct cgggcatcgc gttccagcgc gatgaagctc
     3841 gcatcgccgt gatgggtgtg cccgacaagc cgggcatcgc gtatcagatt ctcggcccgg
     3901 tggcggacgc gaatatcgac gtcgacatga tcatccagaa ccagagcgtc gacggtaaaa
     3961 cggcgttcac gttcacggtt ggccgcggcg actatcagcg cgccatggac atcctcaccg
     4021 gccaggtgaa gggtcacgtg caggctgagc aggtgctggg cgatccgaag gtgtcgaagg
     4081 tgtcggtggt cggcgtcggc atgcgctcgc acgtgggtat cgcgagcacg atgttccgca
     4141 cgctgtcgga agagggcatc aacatccaga tgatctcgac ctccgaaatc aagatttcgg
     4201 tgctgatcga cgagaagtac atggagctcg ccgtgcgcgc gctgcataag gccttcgaac
     4261 tggatcaggc gtaatttgcc gcacgggggt gaggcgtaca agagcgcctc gtctcctggt
     4321 acctggtcgt ggcatgagcg catgcgcggt caatgtggcg cgctaatgtt ggcgcggtaa
     4381 tgtgtcgcgg ccaatgcggc gtggctactg tgacggggct atgtgaacaa ttgcgtcgta
     4441 gatgacaaaa ctggcacaaa aaatgtgcgg tagacattga ccttcgcggt ttggcccgct
     4501 attatcttgg cttcgttgcg ctgactccct gcgcaaccga aagtttggga gacgtggccg
     4561 agaggtcgaa ggcactcccc tgctaaggga gcatctgggc caaaacctgg atcgagggtt
     4621 cgaatccctc cgtctccgcc agcaatggcg gcaaaccccg cagagtctag gctctgcggg
     4681 gttttgtttt ttcaagggcc gctttcccgc tgatgcacac cgaatacgaa taagcgccgg
     4741 cgctgcaaag taaaaccctc agcatgcttc ggataaccaa ttaaatcggc ggagcgtact
     4801 tccttgcgcc cggcgtaacg agtaaatggc cccctttcgt tacagaccgg ctggcttttc
     4861 accgaaatat ccgcgtatgt aaccagacgt aacgcagagg tccacaatgc gattttatgc
     4921 atcagttatt acaaagttga aatacgcgga tgattgcctt gcgctatatt tcggccaaca
     4981 acacacattc ttcaaaaggg tgaatcgatg aagtccgcac gtcttgttcc acttctgttc
     5041 ggggttctcg ggttagctgc gtcgggtgct gcaatggccg gcgggctcaa cgtcggggtc
     5101 aacattggca ttcccgcgcc ggtctatgtc gcgccggctc ccgtctacgc accgccgccg
     5161 cccccgccgc cgtcggtcgt atatcaaccg gctcccgtgt actacggcgg ctcggcgatc
     5221 gtgatcggct ggcacggcga ccgctactgg gacggccgcc gctattgggc gcgtgacgac
     5281 tggtatcgcc accatcctcc gggccgctac gattacggcc gtaatcatta cgacgaccat
     5341 cgcggctggc attaagtcat caagaagccg cggtcgtcgc gtcaaaagaa accgcccgga
     5401 aaagggcggt tttttattgc gctcgcgaag cctgccgatc gatccgccgt cgaccaggaa
     5461 acgcgacacg cattgtcact cggcgacggc aaccatgccc ccgaccacgg cattaaagcc
     5521 gctatccaca tgcacgattt ctgccgtcac accagctgcc ag
//
LOCUS       DATASH010000007         2463 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3956950,
            whole genome shotgun sequence.
ACCESSION   DATASH010000007 DATASH010000000
VERSION     DATASH010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3956950"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..536
                     /locus_tag="VHV99_00195"
     CDS             132..536
                     /locus_tag="VHV99_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX3633921.1"
                     /translation="MQSKAVLTVVEATRILEAGRAEAEKNKWAVTIAVVDDGGHLLAL
                     LRLDDCAPIGAYIATEKARTSALGRRESKQYEDMINGGRTAFLSAPLAGTLEGGVPVV
                     VDGNVVGAVGVSGVKPDQDSQVARAAANSLNG"
     gene            569..>2463
                     /locus_tag="VHV99_00200"
     CDS             569..>2463
                     /locus_tag="VHV99_00200"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HEX3633922.1"
                     /translation="MGEMIERNGLRVAAVLSKFVDDEALSGTGIDSEAFWKGFDALVH
                     DLAPKNRALLAERDRLQTELDRWHRANPGPVRDLKAYRSFLEGIGYIVPVSAAVKATT
                     QNVDTEIAGQAGPQLVVPLSNQRYALNAANARWGSLYDALYGTDAIPETDGAERTAYF
                     NPVRGARVIGRARAFLDEAAPLANGSHVDAIAYRVECGKLAVTLNNGSSALKVPAQFI
                     GYQGDAGSPSAVLLKHHGLHFEIQIDATDSIGKTDAARVKDVLMEAAVSTIIDCEDSV
                     AAVDAAEKVQLYRNWTGLAKGDLSEQVTKNGKTFTRRLNADRKYTAPDGSTVKLHGRS
                     LLFIRNVGHLMTNPAIVDRDGREIPEGILDAVMTSLGAMHDLQNKLNSRTGSIYIVKP
                     KMHGPAEVAFSNELFDRVEDLLGLPRHTIKMGIMDEERRTSVNLQACIAAASARVAFI
                     NTGFLDRTGDEMHSTMQAGPMMRKGDMKSSAWIAAYERNNVLAGLSSGLRGRAQIGKG
                     MWAMPDLMHAMLEQKIAHPKAGANTAWVPSPTAATLHALHYHQVDVQAVQQELEKTAY
                     DSVRDELLDGLLTIPVVEKAQWSDEEIREEIENNAQGILGYVVRWVDQGVGCSKVPDI
                     HDIGLME"
BASE COUNT          525 a          737 c          776 g          425 t
ORIGIN      
        1 atttatgcgg tgcacgtcaa cacgcagggc gcgctgggcg ggtagcccgg ttcaccacga
       61 aacgtcttca ggccaccggt atgcaccggt ggcgacactt cccttcgaaa cacagacttg
      121 ataaccccga catgcaaagc aaagccgtat tgaccgttgt agaagctacg cgaatcctcg
      181 aagccgggcg cgcggaagca gagaaaaaca agtgggcagt caccatcgcg gttgtggatg
      241 acgggggtca tctgctggcc ctgctccgcc tcgacgactg tgcgccgata ggcgcttaca
      301 tcgcaaccga aaaggcgcgt acctcggcgc tcggccgacg tgaatcgaag caatacgagg
      361 acatgatcaa cggcggccgg accgcgttcc tgagcgcgcc gctggccggc acgctcgagg
      421 gcggcgtccc ggtcgtggtc gacggaaacg ttgtgggcgc agtcggcgta tccggtgtca
      481 agccggatca ggattcgcaa gtggcccgag ccgcagcaaa cagcctcaac ggctgattga
      541 ttgatatttg aaactgttgg agcacatgat gggtgaaatg atcgagcgaa acgggctgcg
      601 tgtcgcggct gttctcagca aatttgtcga cgacgaggcg ttgagtggga ctggcatcga
      661 cagcgaagcc ttctggaaag gcttcgatgc cctcgtccac gaccttgccc cgaagaaccg
      721 cgcgctgctc gccgaacgcg atcgtctgca gacggaactg gaccggtggc accgcgcaaa
      781 tccggggccg gtgcgcgatc tcaaggcata tcgctcgttt ctcgaaggca tcggctatat
      841 cgtgccggtg agcgcggccg ttaaggcgac cacccagaac gtcgacaccg aaatcgccgg
      901 acaagccggt ccgcaactcg tcgtgccgct ctcgaaccag cgctacgcgc tcaacgccgc
      961 gaatgcgcgc tggggtagcc tgtacgacgc gctctacggc accgatgcga tccccgaaac
     1021 cgacggcgcg gaacgcaccg cttacttcaa cccggtccgc ggtgcacgcg tgatcgggcg
     1081 cgcccgcgca ttccttgatg aagccgcacc gctcgccaac ggctcccacg tcgacgccat
     1141 cgcgtaccgc gtggaatgcg gcaagctcgc cgtcacgctc aacaacggtt cgtcggcatt
     1201 aaaggttccc gcgcagttca tcggctatca gggcgatgca ggctctcctt ccgccgtcct
     1261 gctcaaacat cacggcctgc acttcgagat tcagatcgac gcaacggact ccatcggcaa
     1321 gaccgacgca gcgcgcgtga aagacgtgct gatggaagcg gccgtctcga cgattatcga
     1381 ttgcgaagac tcggtcgctg ccgtcgatgc agccgaaaag gttcagcttt accgcaactg
     1441 gactggcctc gcgaaaggcg atctgagcga gcaagtcacg aagaacggca agaccttcac
     1501 acgtcgcctg aacgccgatc gcaaatacac cgcgcccgat ggctcgaccg tgaagctgca
     1561 tggccgctct ttgctgttca tccgcaacgt cgggcatttg atgacgaatc cggcgattgt
     1621 cgatcgcgac ggtcgtgaga ttcccgaagg cattctggat gccgtgatga catcgctggg
     1681 cgcgatgcac gacctgcaaa acaagctcaa ttcgcgcacc ggatcgattt acatcgtcaa
     1741 gccgaagatg cacggcccgg ccgaagtcgc gttttccaat gagctgttcg accgcgtcga
     1801 agatctgctg gggctgccgc gccataccat caagatgggc atcatggacg aggagcgccg
     1861 cacgagcgtc aacctgcagg cgtgcattgc ggcggcatcg gcgcgcgtgg cgttcatcaa
     1921 tacgggcttc ctggatcgca ccggcgacga gatgcacagc acaatgcaag cgggtccgat
     1981 gatgcgcaag ggcgatatga aatcgtccgc gtggatcgcg gcttacgagc gcaataacgt
     2041 gctggccggc ttaagctcgg gattgcgcgg acgcgcgcag atcggcaagg gcatgtgggc
     2101 gatgccggac ctgatgcacg cgatgctcga acagaaaatc gcgcatccga aagcgggcgc
     2161 gaacacggcc tgggtgccgt cgccgactgc cgcgacgctg cacgcgctgc actaccatca
     2221 agttgacgtt caggccgtgc agcaagagtt ggaaaagacc gcctatgaca gcgtacgcga
     2281 tgagttgctc gatggcttgc tgacgattcc tgtggtcgaa aaggcgcagt ggtcggacga
     2341 ggaaatccgc gaggaaatcg agaacaacgc gcaagggatt ctggggtacg tggtgcgctg
     2401 ggtcgatcag ggcgttggat gctcgaaggt gccggatatt cacgatatcg gtctgatgga
     2461 aga
//
LOCUS       DATASH010000008         5273 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2572447,
            whole genome shotgun sequence.
ACCESSION   DATASH010000008 DATASH010000000
VERSION     DATASH010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5273
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2572447"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VHV99_00205"
     CDS             complement(<1..379)
                     /locus_tag="VHV99_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX3633923.1"
                     /translation="MSNLNSQTVLSVHHWTDTLFSFTCTRDPSFRFENGQFTMVGLEV
                     DGKPLIRAYSLASANYEEHLEFLSIKVQDGPLTSRLQHLKVGDEVLIGKKPVGTLMAD
                     NLLPGKTLWLLSTGTGLAPFMSII"
     gene            complement(966..1925)
                     /locus_tag="VHV99_00210"
     CDS             complement(966..1925)
                     /locus_tag="VHV99_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3633924.1"
                     /translation="MKKNLPILLALVALIVLGLVRYEHFGSAYNITSFWRYNSMFALI
                     SIGMAFVIITGGIDLSVGTVAALASVVAALTSVYGGWVAVVAGCAAGMAVGVLNGLII
                     TRLKILPFIVTLATSLGAHGVALLLGKNDAVSIAADSNFGNFGQGDLFGLPIPGIVAV
                     VAAVAGWLALRSTRFGRHSLAIGGSEEAARLMGLNVDRTLVMAYAVSGLLAGMAGVIL
                     AAQFGAGQPNEGVGWELFAISAVVLGGTLLTGGEGSIAMTIAGVLLLGLVFNLLNFEN
                     GLGFISLSAYWQSVIRGVFLLLVIVLQARVLKQRGKKRTAAPA"
     gene            complement(1962..2990)
                     /locus_tag="VHV99_00215"
     CDS             complement(1962..2990)
                     /locus_tag="VHV99_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3633925.1"
                     /translation="MALKLHTESLRNETAPPLPATAAPRATAASTVKKWRHLAMQREV
                     IVLLAMVLFNLILTPHFWSLQTFNVNMTQVVTIVIVGIGMTLVVATGGIDLSVGASMA
                     ISGALAPMLFLNIPGPGGIALAFVLPVLAAAACGVFNGLLVTRLSVQPIVATLVLFIA
                     GRGIAQVVTDGSLQAFNTPAFQWIALGKVAGVPFQVLLMLALVVLFAWIVRKTLFGQY
                     LLITGGNEKAAYLCGVPTATVKLIAYTLCAALAGLAGLISISVNSSSDANVVGLGVEL
                     DAIAAVAVGGTALTGGKAYIGGTLIGALIIQLLRYTLLAHGIPDAAALVVKAGIIVAA
                     VYVQRRSR"
     gene            complement(2980..4545)
                     /locus_tag="VHV99_00220"
     CDS             complement(2980..4545)
                     /locus_tag="VHV99_00220"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEX3633926.1"
                     /translation="MQDIGISFGGVPALRSANLSVAAGEVHALIGQNGAGKSTMIKIL
                     TGAYRRGSGTVRFEGREVDFRTPEQAREAGISTIYQEINLVPFRSVAENIFLGREPRR
                     FGLIDWRAVQQRAAALLESFGLQIDVKKPVSRYSTAIQQMVALARAVSSDAKMVIMDE
                     STSSLDEREVELLFTVVRKLRDDGRAVIFVSHRLDELYALCDRVTVMRDGQTVAQSTM
                     ADMDKLQLVTTMLGRTLAAVVQDDTVAREANLARRGKLAIAATHLSTHPKVSDVSLEV
                     HAGEAVGLAGLLGSGRTETMRLMFGADPLEQGSLSIGGERVTFKSPQDAIARGLAYLT
                     EDRKAEGIVPELSVRDNLTLVCLRTLAKHGVVDVKKQQAIVDRFIASLGIKLRSADQP
                     IRELSGGNQQKVLLARWLAAEPSLLLLDEPTRGIDVGAKADVAKIVRELRDAGLAVLL
                     SASELEELTAVADRAVVIRDGRTVAELNGADMSETAIMDAIAYGSEGQSQLAEAAQSA
                     HIEDALEGDRHGA"
     gene            complement(4983..>5273)
                     /locus_tag="VHV99_00225"
     CDS             complement(4983..>5273)
                     /locus_tag="VHV99_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX3633927.1"
                     /translation="AHNDEMALGAIAAIKAAGKQPGKDIQIVTIDGTKGGMDAIAAGE
                     LGASVQSSPFFGPLACDVAQRYAKGEKIPTWVKVSDRFYDKSNVQQNMQYGY"
BASE COUNT          959 a         1719 c         1699 g          896 t
ORIGIN      
        1 tgatgatgga catgaacggc gcgagacccg tgccggtgga cagcagccac agcgtcttgc
       61 cgggcagcag gttgtcggcc atcagcgtgc cgaccggctt cttgccgatc aggacttcgt
      121 cgccgacctt cagatgctgc aagcgcgacg tgagcgggcc gtcctgtacc ttgatgctca
      181 ggaattcgag gtgttcttcg tagttcgcac tcgcgaggct gtaggcgcgg atcagcggct
      241 tgccgtcgac ttcgaggccc accatcgtga actggccgtt ttcgaagcgg aacgacgggt
      301 cgcgcgtgca ggtgaagctg aaaagcgtat cggtccagtg atggacgctc aggacggttt
      361 gtgaattcag gttgctcatg gcttcttgga tagtgcgaaa acaaaggcgg ccagtcgttt
      421 tagccggccg gcacggcaag ggcgatgctg cgctaacccg aggtcgggaa tcacgcgcaa
      481 tccgctattt taccgcgccc gttgcccgag ggctgcggcg cggtggttga gcgtggtgaa
      541 gcgggttacc ggacctggaa gcgttggcgc cgatgtcggt acgagcggcg ccgcgaagta
      601 cgttggcggg gctgcggccg cgcttcaacc agggttcggc gatgggcgaa agccgcgcag
      661 ggcggctgcc atcggctgtc cggcatgcgc cggataggcg cgccgcgcgg gcaacatttt
      721 caggatgata ccgaaagctg ccatgcgccg cagcattgac cctgctgata aaactggcaa
      781 aagcgggaga aaccggcggc aaaggcgagg aatcggcccg agtcggccga catgaaccgt
      841 ccgcatccgg caaggctagc ggccgtgaga ttacgtcgcc gagttggtac acaaaggtgg
      901 aattgtggac gaaataaccg ccggaaggtc aatctacata agggtttaag cgccgctcgg
      961 tcaggtcacg ccggcgccgc cgtacgcttc ttgccgcgct gcttcaacac tcgcgcttgc
     1021 agcacgatca ccagcagcag gaacaccccg cgaatcaccg actgccagta cgccgacaag
     1081 ctgataaatc caagcccgtt ctcgaagttc agcaagttga acaccagacc gagcagcaac
     1141 acgccggcaa tcgtcatcgc aatcgagcct tcgccgccgg tgagcaaggt gccgccgagc
     1201 accaccgccg aaatcgcgaa cagctcccag ccgacgcctt cgttcggctg acccgcgccg
     1261 aactgcgcag cgaggatcac gcccgccatg ccggcgagca ggccgcttac cgcataagcc
     1321 atcaccagtg tccggtcgac gttcagcccc atcagccgcg ccgcttcctc gctgccgcca
     1381 atggccagcg aatgccgccc gaagcgcgtg ctgcgcagcg cgagccagcc ggctaccgcg
     1441 gcgactaccg cgacgattcc cgggatcggc agtccgaaca gatcgccttg accgaagttg
     1501 ccgaagttcg agtcggcggc aatcgacacc gcgtcgttct tgccgagcag cagcgcgacg
     1561 ccgtgcgcgc cgaggctggt cgcgagcgtc acgatgaagg gcaggatttt cagccgcgtg
     1621 atgatcaggc cgttgagcac gccgacggcc atgcccgccg cgcaaccggc tacgacggca
     1681 acccagccgc cgtagacgct ggtcaacgcc gcgacgacgc tcgccagagc ggccacggtg
     1741 ccaaccgaga gatcgatgcc gcccgtgatg attacgaagg ccatgccgat cgagatcaac
     1801 gcgaacatcg agttgtagcg ccagaaggac gtgatgttgt aggccgagcc gaaatgctcg
     1861 tagcgcacga ggccgagcac gatgagcgcg acgagcgcaa ggaggatagg aaggttcttt
     1921 ttcatcgcat cgaatccggg agggttcctg aagcgtgagg gttagcgcga gcgccgctgc
     1981 acatacaccg cggcgacgat gatgccggcc ttcaccacca gtgccgccgc gtcgggaatg
     2041 ccgtgcgcga gcagcgtgta gcgcagcaac tggatgatca gcgcgccgat cagcgtgccg
     2101 ccgatatagg ccttgccgcc cgtcagcgcc gtgccgccga ccgccaccgc ggcgattgca
     2161 tcgagttcga cgccgagtcc caccacattc gcatcggacg acgaattcac cgagatcgag
     2221 atcagccccg ccaggcctgc gagcgcggcg cagagcgtgt aggcgatcag cttcacggtg
     2281 gccgtcggca caccgcacag ataggcggcc ttttcgttgc cgccggtgat caacaggtac
     2341 tggccaaaca gggtcttgcg cacgatccac gcgaacagca cgaccagcgc cagcatcagc
     2401 agcacctgga acggcacgcc ggcaaccttg cccaatgcga tccactgaaa cgccggcgtg
     2461 ttgaacgcct gcaagctgcc atccgtgacg acctgagcga tgccgcgccc ggcgatgaac
     2521 agcaccagcg tcgcgacaat cggctggacg gagagcctcg tcaccagcaa gccgttgaag
     2581 acgccgcacg cggccgccgc cagcaccggc agcacgaacg cgagtgcaat gccgcccggc
     2641 cccggaatgt tcaggaacag catgggcgcg agcgcgcccg agattgccat cgacgcgccc
     2701 accgacaggt cgatgccgcc cgtcgccacc accagtgtca tgccgatgcc gacaatcacg
     2761 atcgtcacca cctgcgtcat gttgacgttg aaggtttgca gcgaccagaa gtgcggcgtg
     2821 aggatcaggt tgaacagcac catcgcaagc agcacgatca cttcgcgttg catggcgagg
     2881 tgccgccatt tcttcacagt cgacgcggcc gtggcgcgcg gtgcggcggt ggccggcagc
     2941 ggcggtgcgg tttcgttgcg cagcgactcg gtgtgcaact taagcgccat gacggtcgcc
     3001 ctccaacgcg tcttcgatat gggcggactg ggcggcttcg gccagttgcg actggccttc
     3061 gctgccgtag gcgatggcgt ccatgatcgc ggtctcgctc atgtcggcgc cgttcagttc
     3121 cgcgacggtg cggccatcgc ggatcaccac cgcgcggtcg gccaccgccg tcagttcttc
     3181 gagttccgac gcggacagca gcacggcgag gcctgcgtcg cgcaattcac gcacgatttt
     3241 cgccacgtcg gctttggcgc cgacgtcgat gccgcgcgtc ggttcgtcga gcagcagcag
     3301 cgacggttcg gcggcgagcc agcgcgcgag caggaccttc tgctgattgc cgccggagag
     3361 ttcgcgaatc ggctgatccg ccgagcgcag cttgatgccg agcgacgcaa tgaaacggtc
     3421 gacgatcgcc tgctgtttct tcacgtcgac tacgccgtgc ttcgccaacg tgcgcaagca
     3481 gaccagcgtg aggttatcgc gcaccgagag ttcggggacg atgccttcgg ctttgcggtc
     3541 ctcggtgagg taagcgaggc cgcgcgcgat cgcatcctgc ggcgacttga acgtcaccct
     3601 ctcgccgccg atcgacaagg aaccttgttc gagaggatcg gcgccgaaca tcaggcgcat
     3661 agtttcggta cggcccgagc cgagcaggcc cgcgagaccc accgcttcgc ccgcatggac
     3721 ttcgagcgag acgtcgctca ccttcgggtg cgtgctcagg tgggtcgcgg cgatcgccag
     3781 cttgccacgc cgcgccagat tcgcttcgcg tgccacggtg tcgtcctgca cgacggcggc
     3841 aagcgtgcgg ccgagcatcg tcgtgacgag ttgcagtttg tccatatccg ccatcgtgct
     3901 ttgcgcgacc gtctggccgt cgcgcatcac ggtgacgcgg tcgcatagcg catacagttc
     3961 gtcgagccgg tgcgagacga agatcaccgc gcgtccgtcg tcgcgcagtt tgcgcaccac
     4021 ggtgaagagc aattccactt cgcgttcatc gagcgacgag gtggactcgt ccatgatgac
     4081 catcttcgca tccgacgaca ccgcgcgcgc cagcgccacc atctgctgga tcgcggtcga
     4141 atagcggctg accggtttct tcacatcgat ctgcaagccg aacgattcga gcagcgcggc
     4201 ggcgcgctgc tgcactgcgc gccagtcgat caggccgaaa cggcggggtt cgcggccgag
     4261 gaaaatgttc tccgccaccg agcggaacgg caccagattg atctcctgat agatcgtgct
     4321 gatgccggct tcgcgcgcct gttcaggcgt gcggaaatcg acttcccggc cctcgaagcg
     4381 caccgtgccc gagccgcgcc gatacgcgcc ggtcaggatc ttgatcatgg tcgacttgcc
     4441 ggcgccgttc tggccgatca gcgcatgcac ttcgccggcc gcgacgctca gattggcgct
     4501 gcgcaacgcg ggtacgccgc cgaagctgat gccgatatcc tgcatctcca gcagcggcga
     4561 acgggtaagg ggagagtgtg gcgtctcttt gccggattcc gtcacgggtg tcctcctgat
     4621 gcgtgtgctg cctcatgtgc cgccgtgtgc tgcatgcgtt gcacgtgcga gctgctgtgg
     4681 cgattcgttc gggctcttat gcgggcgcta gcgtcgcatc atgccgaccc atgttacgcc
     4741 gcataaaagc gaagcgacgg cccgtgctgc cacgaacgcg tcgctccgaa cccttcccgt
     4801 cgcccgccct ccgaggagac agattcgggc ggggacgtcc tgcgaggcaa tccctcgatc
     4861 ccgatgccca aacgggactt gcttcggggc ggaggacttc cttcggatcg cggagcgcgc
     4921 ggacgctccg ctacggcaca acgctcaaac ttcaaaaccg cgcatagagg cgcgcttcgc
     4981 tatcaatagc cgtactgcat gttctgctgc acgttgctct tgtcgtagaa gcggtcggac
     5041 accttgaccc acgtcgggat tttttcgccc ttcgcgtaac gctgtgcgac gtcgcaggcg
     5101 agcgggccga agaacgggct cgactgcacg ctcgcaccga gttcgccggc ggcgatcgcg
     5161 tccatgccgc ccttggtgcc gtcgatcgtg acgatctgga tgtccttgcc cggctgcttg
     5221 ccggctgcct tgatcgcggc aatcgcgccg agtgccattt cgtcgttgtg cgc
//
LOCUS       DATASH010000009         2901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_396491,
            whole genome shotgun sequence.
ACCESSION   DATASH010000009 DATASH010000000
VERSION     DATASH010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396491"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VHV99_00230"
     CDS             <1..304
                     /locus_tag="VHV99_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX3633928.1"
                     /translation="SANTAAASADANSGTPASPPGSRFAVQLGAFANDANARNWAAKL
                     KAAGVPAYTEHRKQADGSTLTLLRAGPFADRAAASAAIAKVREAGLTSGANSGAAQ"
     gene            312..806
                     /locus_tag="VHV99_00235"
     CDS             312..806
                     /locus_tag="VHV99_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004188369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEX3633929.1"
                     /translation="MFTAFDYAVMAVIGLSALRGTWRGFLSEIFGLIGWIAAFFIACR
                     FVGYVVPFIPATWPGGALTQWLLAFALVVVGVVLVASVLNALLSRLVQATGLSGVDRS
                     LGLMFGLVRGVVLVLVLVALAGLTELPQQEFWRNALLRPYAVEGVHVMKPLLPETLAA
                     YVRV"
     gene            942..2492
                     /gene="purF"
                     /locus_tag="VHV99_00240"
     CDS             942..2492
                     /gene="purF"
                     /locus_tag="VHV99_00240"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013342047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX3633930.1"
                     /translation="MCGIVGVVSHSPVNQLIYDSLLLLQHRGQDAAGIATANGSNFHM
                     HKANGMVRDVFRTRNMRSLPGTTGIGQVRYPTAGSASSEEEAQPFYVNAPFGIILAHN
                     GNLTNWQQLKDEMFRIDRRHINTNSDTEVMLNVLAHELQLSSSGLQLDPAALFNAVSG
                     VHRRVRGSYAIVSLIAGYGLLGFRDPFGIRPLCLGKQETAEGVEWILASESVAIEGIG
                     FEFVRDIAPGEAIFIDIEGNLHSQQCATNPSLNPCIFELVYLARPDSVLDGVPVYNVR
                     LRMGDYLAEKIRRELPDVAIDVVMPIPDSSRPAAMQVAKKLGVEYREGFFKNRYVGRT
                     FIMPGQAVRKKSVRQKLNAMGIEFKGKNVLIVDDSIVRGTTSHEIVQMARDAGANKVI
                     FASAAPPVKYPNVYGIDMPTRGELVAHGRTDDEVARMIGADHLVYQDVDALKQAVRDI
                     NPALKQFEASCFDGNYVTGDVTTEYLDRIETARLAPSSQSDRDAASEAIDGGAPARSQ
                     LHLQLSVG"
BASE COUNT          533 a          896 c          896 g          576 t
ORIGIN      
        1 cagcgcgaat actgccgccg ccagcgcgga cgcgaattcc ggcacgccgg cgtcgccccc
       61 gggtagccgt ttcgccgtcc agctcggcgc atttgcgaac gacgccaacg cgcgcaattg
      121 ggccgccaag ttgaaagcgg ccggtgtgcc cgcatatacc gaacatcgga aacaggccga
      181 cggctcaacg cttacgctat tgcgcgccgg tccgttcgcc gaccgtgcag cggcgagcgc
      241 cgcgattgca aaggttcgcg aggccggctt gacgtcgggc gcgaacagcg gcgcggcaca
      301 gtaagcgagc gatgttcact gccttcgact acgctgtaat ggcggtgatc ggtttgtcgg
      361 ccttgcgcgg cacatggcgc ggctttttgt ctgagatatt cgggctgatc ggctggattg
      421 cggcgttttt cattgcttgc cgcttcgtcg gttatgtcgt gccgtttatc cccgccacgt
      481 ggccgggcgg cgcgctgacc cagtggctcc tggcgttcgc gctggtggtg gtcggcgtgg
      541 tgctggtggc aagcgtgctg aacgcgttgc tgagtcgcct tgtgcaggca accggcttga
      601 gcggcgtgga ccgctcgctc ggtttgatgt tcggcctcgt gcgcggggtc gttctggtgc
      661 tggttctggt cgcccttgca ggcttgaccg aactgcccca acaggaattc tggcgcaacg
      721 ccttgcttcg accctatgcg gtcgagggcg tgcacgtaat gaaaccgctg cttcccgaga
      781 cgctcgctgc ctacgtccgc gtgtgacgat caagtgaacg gatagaggaa cgcagcagga
      841 ctccggcgca atccggtacc tctatatacg gtcgcgtacc ctcgcgcagc atcgcaggta
      901 tcggccgccg ttacgaattt tgtacctttg aaggacatgc catgtgcggc atcgtaggcg
      961 tagtttccca ctctccggtc aatcagctga tctatgacag cctgctgctt ctgcagcatc
     1021 gcggtcagga cgccgccggc atcgcgacag cgaacggcag caatttccac atgcacaagg
     1081 ccaacggcat ggtgcgcgac gtgttccgca cgcgcaacat gcgtagcctg cccggcacca
     1141 ccggtatcgg ccaggtccgt tacccaaccg ccggttccgc atcgagcgaa gaagaagccc
     1201 agccgttcta cgtgaatgca ccgttcggca tcatcctcgc gcacaacggc aatctgacca
     1261 actggcagca gctgaaagat gagatgttcc gcatcgatcg ccgccacatc aataccaatt
     1321 ccgacaccga agtgatgctc aacgtgctcg cgcacgaatt gcaactgtcc agctcgggtc
     1381 tgcaactgga tccggccgcg ttgttcaacg cggtgtcggg cgtgcatcgc cgggtgcggg
     1441 gttcgtacgc gatcgtctcg ctgattgccg gctacggttt gctcggtttc cgcgacccgt
     1501 tcggcattcg ccctttgtgc ctcggcaagc aggaaacggc tgaaggcgtc gagtggattc
     1561 tggcgtcgga atcagtcgcg attgaaggta tcggtttcga attcgtgcgc gatattgcgc
     1621 cgggcgaggc cattttcatc gacatcgaag gcaatctgca ttcgcagcaa tgcgcgacga
     1681 acccgagcct gaatccctgc attttcgaac tcgtgtatct cgcgcgtccg gactcggtgc
     1741 tcgacggcgt gccggtctac aacgtgcgtt tgcgcatggg cgattacctc gccgagaaga
     1801 tcaggcgtga attgcccgac gtcgcgatcg acgtcgtgat gccgatcccc gattcgtccc
     1861 gtccggccgc gatgcaggtc gcgaagaaac tgggcgtgga gtatcgcgaa ggcttcttca
     1921 agaaccgtta cgtcggccgc accttcatca tgccgggcca ggccgtgcgc aagaagtcgg
     1981 tgcgccagaa gctgaacgcc atgggcatcg agttcaaggg caaaaatgtg ctgatcgtcg
     2041 acgattcgat cgtgcgcggc acgacctcgc acgaaatcgt gcagatggca cgcgatgccg
     2101 gcgcgaacaa ggtgatcttc gcttcggcgg cgccgccggt gaagtacccg aacgtctacg
     2161 gtatcgacat gcccacgcgc ggtgaactcg tcgcacatgg ccgtacggac gacgaagtcg
     2221 cgcgcatgat cggggcggac catctcgttt atcaggacgt tgacgcgctg aagcaggcgg
     2281 tgcgcgatat caacccggcg ctgaagcaat tcgaagcctc gtgttttgac ggcaactacg
     2341 tcacgggtga tgtcacgacc gaatacctcg accgtatcga aaccgcgcgt ctcgcaccgt
     2401 cctcgcaatc ggatcgcgac gccgcgagcg aagcgatcga cggcggcgcg ccggcgcgtt
     2461 cgcaattgca tctgcagttg tcggtgggct aaaagcgata tggctcgcgg cgtgacgtgc
     2521 ccggctgcac gccacgccgc gagcgtgtta gcatgacggc ttgcgtcgat ttgatctcag
     2581 cttgcggacg cggggcaacc caaaacagct aaagcgaagg gtggaaaagc cgcagtcatc
     2641 cgcctccgct ccagctttcc cccgcacatg aagcccgctt atgccgacgc aaaagcgggc
     2701 ttttttgttg ttgccgtttg gcgtcacggg ctcctttcac gtgtgccgtc gacaccgaac
     2761 ccagccatgg aacattggaa accagaacca acatggacga ctccctgaac ttcgatacgc
     2821 tggcagtccg ctcgggcaca gcgcgcagcg acttcaacga acactcggaa gcgattttcc
     2881 tgacctcgag cttcgtcttc g
//
LOCUS       DATASH010000010         3259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5341786,
            whole genome shotgun sequence.
ACCESSION   DATASH010000010 DATASH010000000
VERSION     DATASH010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5341786"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="VHV99_00245"
     CDS             <1..825
                     /locus_tag="VHV99_00245"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEX3633931.1"
                     /translation="NAHHVLRLTPIDRRDQRVHAAALDITPAPVERREGQDFFGNRIT
                     WIKLDAPHETLTVRVAARIAVDHTEAVAAETTPPWEAVREAAFGCNDLSPQAPAHYLF
                     ASRLVSLDPEIRDYAAESFAPGRPVLEGAMALMNRIKADFVYEPGATTASTAPPMSFA
                     LRRGVCQDFSHVMISGMRSLGLPAAYVSGYLRTSRVQGETKLEGADAMHAWVLAWCGE
                     EAGWIGLDPTNGMLAGNDHVVLAVGRDYPDVAPIDGVIVAAGGQRLEVSVQVVPVA"
     gene            1262..2422
                     /locus_tag="VHV99_00250"
     CDS             1262..2422
                     /locus_tag="VHV99_00250"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX3633932.1"
                     /translation="MPTVTLSAPFVRQAQCLAGRKKTDYFDAGQRGLLLEVRQSGGKT
                     YYQRYTDERGRERQYKIGPTDVITLEQARRKGRQVVAEALLGADPQQKRREMRAVPQL
                     KDFVAERYLPYVRTYKSSWQTDETVLRIHILPKLGRLALDEIKTDAISDLINCMREDG
                     YASGTMNRVIVILRYMYNLARKWKVPGGLENPAAGLTAGPDVQRNRFLTEEEAEALVR
                     AIHADENQTAARSIMLLLLTGARRNEITFARWDYIDYKNKTLLVPKSKSGRARVIALN
                     QSAIALLTSTPRLDGNPYIFPSPVTGRPSASLFFPWDRIRARAGLQDVRLHDLRHSFA
                     SFLVNRGVSLYVVQGLLGHLHARTTQRYAHLTQETLIGAADLIQDVVTFRGA"
     gene            2874..>3259
                     /locus_tag="VHV99_00255"
     CDS             2874..>3259
                     /locus_tag="VHV99_00255"
                     /inference="COORDINATES: protein motif:HMM:NF014577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX3633933.1"
                     /translation="MKICSLYGAGFYYIPGTDMCLKIGGWVRFEAANPVGGNISLGAF
                     NGGYNNRATQNSTWRARGYITADARNQTEYGTVRSYIAVGVNTNSVGLDNTANQFSAN
                     RAFIQWAGFTFGLAQSFFDFYSVPAIS"
BASE COUNT          591 a         1014 c         1026 g          628 t
ORIGIN      
        1 aacgcgcacc acgtgctgcg cctgacgccg atcgaccgcc gcgaccagcg cgtgcacgcg
       61 gccgcgctcg atattacgcc ggcgccggtg gagcggcgcg aagggcagga tttcttcggc
      121 aaccgcatca cgtggatcaa gttggacgcg ccgcacgaga cgctgaccgt gcgcgtcgcc
      181 gcgcgcatcg ccgtcgacca cacggaggcg gtggccgcgg aaacgacgcc gccctgggaa
      241 gcggtgaggg aggccgcgtt cggctgcaat gacttgtcgc cgcaggcgcc ggcgcattac
      301 ctgttcgcga gccggctggt gtcgctcgat cccgagatcc gcgactacgc cgccgagagc
      361 tttgctcccg ggcggcctgt gctggaaggt gcgatggcgc tgatgaatcg catcaaagcc
      421 gatttcgtct acgagcccgg cgccaccacc gcgagcacgg cgccgccgat gtccttcgcg
      481 ctcaggcgcg gcgtgtgtca ggacttttcg cacgtgatga tctccggcat gcgctcgctc
      541 ggcctgccgg cggcctacgt cagcggctat ttgcgcacca gccgcgttca gggcgagacg
      601 aagctcgaag gcgccgacgc gatgcacgcc tgggtgctgg cctggtgcgg ggaagaggcg
      661 ggctggattg ggctggatcc gaccaatggc atgctggccg gcaacgatca tgtggtgctg
      721 gcggtcggcc gcgattatcc ggacgtcgcg cccatcgatg gcgtcatcgt cgcggccggc
      781 ggccagcgcc tggaagtctc cgtgcaggtc gtgccggtcg cctaacgttc gcgggcgcgg
      841 cggttgctcg ccacatctca cgttatcccc aacgacgcga tagaggtgcg ctatgtgggt
      901 ggccatgccc actatgcgcc gatgtgggcg caggcttggc agacaaatgc gttctcgttc
      961 cgaaaaagac ggctggactt taggtgtaca taccctggta acgtcctact tttcctagaa
     1021 tattgtgttt actgcacatt taaggttgcg cggaccacgt tatccactgt tttcataagg
     1081 ctttgattcg atgacataaa cgtcccgtaa atcatcattt ataaacatga gatcataaat
     1141 gatgatttac accgctaagt atctagagtt tcataagaag taacgaataa ttaatagctg
     1201 caccggggaa ggctgtggtt catctccgcc cgggggtgga gatgcgtaac agtaattacg
     1261 aatgccaacc gtcaccttgt ctgcaccctt cgtgcggcag gcgcagtgcc tcgctggccg
     1321 caagaagacc gactatttcg acgcgggcca gcgggggctc ctgctcgagg tccgtcaatc
     1381 cggcggcaag acctactatc agcgctacac cgacgagcgc ggccgcgagc ggcaatacaa
     1441 gatcggtccg acggacgtca tcacccttga gcaggcacgc cgcaaagggc gccaagttgt
     1501 cgccgaggcg ctgctcggcg ccgacccgca gcagaagcgc cgcgagatgc gcgccgtgcc
     1561 gcagctcaag gacttcgtgg cggagcgcta tctaccttat gtgcgcacgt acaagagcag
     1621 ttggcagact gatgagacgg ttctgcgcat ccacatcttg ccgaagctcg gccgcttggc
     1681 gctggacgag atcaagacgg acgccatctc cgatctgatc aactgcatgc gtgaggacgg
     1741 ctacgcctcc ggcacgatga accgggtgat cgtgattctg cgctacatgt acaatcttgc
     1801 ccgcaaatgg aaggtgccgg gaggattgga aaacccggcg gctggtctga cggcggggcc
     1861 ggacgtgcag cgcaaccgct tcctcaccga agaggaggcc gaggcgctcg tgcgcgcgat
     1921 ccacgctgac gagaaccaga cggcggcgcg ttcgatcatg ctgctgctgc tcaccggcgc
     1981 gcggcgcaac gagatcacct tcgcgcgctg ggactacatc gactacaaga acaagacgct
     2041 gctggtgccg aagtcgaaat cggggcgcgc gcgcgtgatc gcgctcaacc aatcggccat
     2101 cgcgctgctg acgtcgacgc cgcgcctgga cggcaaccct tacatcttcc cgtcgccggt
     2161 caccgggcgg ccgtcggcct cgctgttctt tccctgggat cgcatccgcg cgcgggcggg
     2221 cctgcaagac gtgcggctgc acgacctgcg ccattcgttc gcgagcttcc tggtgaatcg
     2281 cggggtgtcg ctctacgtcg tgcagggcct gctcggccat ctgcatgcac gcacgaccca
     2341 gcgttatgcg catctcacgc aagagacgct gatcggcgcc gccgatctca ttcaggacgt
     2401 cgtgacgttt cgcggcgcat aattcgagcg cggtgttgca cgcgggcacg caaatgtctt
     2461 gcgcggttcc gcggcaagcc gcgtgcaaca tgccgtcaac ttagcgtctt tgtgatctaa
     2521 ccgcttacgt gcgttcaagc gcgcgtgctt tgtgcacgcg cgcttttttg cgtccgttta
     2581 ccgaaaaata accagcgtcg gatgaagtgc gaacgcgcgt tcgacgcgtc gtttcgtgcg
     2641 gccgtacaag acttttttgc gcgcgtccca ggctataccc atatcgcatc gctcgacgtc
     2701 gtgccgcgtt ccgaaaaagt tttgcgtgcg atcaagtgct tggatttcgc gcggacccac
     2761 ggcgaccaca gttggggagg gtgcgcattt gtcggcaaag ccggcgcgcg tcgttgtcgc
     2821 atgcgcgcgt tattggcttt cgcgcaaggt cagacgcgca atccctccag tacgtgaaga
     2881 tttgcagctt gtatggcgcg gggttctatt acatcccggg cactgacatg tgcttgaaga
     2941 tcggtggctg ggtccgcttc gaggctgcta accccgtcgg cggcaatatt tccctcggtg
     3001 cgttcaacgg cggctataac aaccgcgcga ctcaaaacag cacttggcgc gcgcgcggct
     3061 acatcacggc cgatgcgcgt aaccagaccg agtacggtac ggtccgcagc tacatcgccg
     3121 ttggcgtcaa caccaacagc gtgggcctcg acaacacggc caaccagttc agcgcgaacc
     3181 gcgcctttat ccagtgggcc ggcttcacct tcggtctggc gcagtcgttc ttcgacttct
     3241 acagcgtgcc ggcgatttc
//
LOCUS       DATASH010000011         2842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1385798,
            whole genome shotgun sequence.
ACCESSION   DATASH010000011 DATASH010000000
VERSION     DATASH010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1385798"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1246
                     /locus_tag="VHV99_00260"
     CDS             <1..1246
                     /locus_tag="VHV99_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HEX3633934.1"
                     /translation="QVRTRKMHEIAEAGVAAHWLYKNGGADLNDVQKRAHQWLKSLLD
                     IQSEAGDSSEFLEHVKIDLFPDAVYVFTPKSKIMALPRGATALDFAYSIHSDLGNQCV
                     AVKINNELLPLRTELKSGDIVEVITAPYSKPNPAWLGFVRTGKARSAIRHYLKTMRLN
                     ESVQLGERLVDQALKGYGLALSDVTPEAWEKLVQWTGNKNRQEIFADIGLGRRVAAVM
                     AKRIEVLMSGRDADDDGSRDGTASHAPPVVITGTEGMSVQLSACCRPIPGDDIMGYIG
                     IGLGMAIHTTDCRVAQRIHRRDPGRWIDVAWAPQPGRLFDVAIKVLVKNTKGVFARVA
                     ADITSADANIVHIAMDEDLSQESTVLRFVIQVSDRVHLANVMRRVRTNPDVMRIARER
                     PSEEGHHRHDGGMRIDRERADY"
     gene            1384..1872
                     /locus_tag="VHV99_00265"
     CDS             1384..1872
                     /locus_tag="VHV99_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018434470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein NinX family protein"
                     /protein_id="HEX3633935.1"
                     /translation="MNVAELTGTALDYWVARSLHDFVREIHFTDGGETVSIRGIDRGR
                     PWDGRFTPSTSWEAAAAVLERAQRLEVRERTDHGAAHCIAEFEGGHRTVEGRGDSLRV
                     ALLRAFVESRFGDRVDDVLREAQAVTGARAEPIGEELAGGSFEDVPSPDGQIGDIQSA
                     PR"
     gene            complement(1988..2064)
                     /locus_tag="VHV99_00270"
     tRNA            complement(1988..2064)
                     /locus_tag="VHV99_00270"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2028..2030),aa:Arg,seq:acg)
     gene            complement(2140..2216)
                     /locus_tag="VHV99_00275"
     tRNA            complement(2140..2216)
                     /locus_tag="VHV99_00275"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2180..2182),aa:Arg,seq:acg)
     gene            2429..>2842
                     /locus_tag="VHV99_00280"
     CDS             2429..>2842
                     /locus_tag="VHV99_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HEX3633936.1"
                     /translation="MNKAFVKESGDDADDDLDIAQPDVPAGAKNYITPSGHKRLRDEL
                     LHLIDEERPEVVKLVSWAASNGDRSENGDYIYGKRRLREIDRRIRFLTKRLDLAEVVD
                     SSKQENLDQVFFGATVDYATADGETHTVTIVGIESL"
BASE COUNT          566 a          822 c          937 g          517 t
ORIGIN      
        1 ccaggtgcgc acgcgcaaga tgcacgagat cgccgaagcg ggtgtcgccg cgcactggtt
       61 gtacaagaac ggcggcgcgg acctgaacga tgtgcagaag cgcgcgcatc aatggctcaa
      121 gtcgctgctc gacattcaga gtgaagcggg cgattcgagc gaattcctcg agcacgtcaa
      181 gatcgatctg ttcccggatg cggtctacgt gtttacgccg aagtccaaga tcatggcgct
      241 gccgcgcggc gccaccgcgc tcgacttcgc gtattcgatc cacagcgacc tgggtaacca
      301 gtgcgtcgcg gtgaagatca ataatgaact gctgccgctg cgtaccgagc tgaagagcgg
      361 cgatatcgtc gaagtgatca cggcgccgta ttcgaagccg aatccggcgt ggctgggctt
      421 tgtgcgcacg ggcaaggcgc gttcggcgat ccgccattat ctgaagacca tgcggctgaa
      481 cgagtccgtg cagctcggcg agcgtctcgt cgaccaggcg ctgaagggct atgggctggc
      541 gctgtcggac gtcacgccgg aagcgtggga aaagctggtg cagtggacgg gcaacaagaa
      601 ccgtcaggaa atcttcgcgg atatcggcct cggccgccgt gtcgccgcag tcatggccaa
      661 gcgcatcgaa gtgctgatga gcggccgcga tgccgacgac gacggttcgc gcgacggcac
      721 agcgtcgcat gcgccgccgg ttgtcatcac cggcaccgaa ggcatgtcgg tgcaactgtc
      781 cgcatgctgc cggccgattc cgggcgacga catcatgggc tacatcggca tcggccttgg
      841 catggccatc cacaccacgg attgccgcgt cgcccagcgt atccatcgcc gcgatccggg
      901 ccgctggatc gacgtggcgt gggcgccgca gccgggccgc ctgttcgacg tcgccataaa
      961 ggtgctggtg aagaacacca agggtgtttt cgcccgcgtg gcggccgaca tcacgtcggc
     1021 ggatgccaac attgtccaca tcgcgatgga cgaagacctg tcgcaggaat ccaccgtgtt
     1081 gcgcttcgtc atccaggtca gcgaccgcgt ccatctcgcc aacgtgatgc gtcgtgtgcg
     1141 gaccaatccg gacgtgatgc gtatcgcgcg cgagcggccg agcgaggaag ggcatcaccg
     1201 tcatgacggc gggatgcgga tcgaccgcga gcgggcggat tactgatctg acggctggct
     1261 gaggctgctc gacgacgtgg gcgtctgggc ggcgcggtcg gcttcgggtt tcgggtagcc
     1321 ttcgttcgaa tctgaatgca ccccttgcca gcgagtctca gcggaacttc agggagaacg
     1381 cttatgaacg tagctgagct caccggcacg gcgctcgact attgggtcgc ccgcagcctg
     1441 cacgacttcg tgcgcgaaat ccactttacc gacggcggcg aaaccgtctc gatccgcggc
     1501 atcgaccgcg gccgtccatg ggatggccgt tttacgccgt ccacgtcatg ggaagcggcc
     1561 gccgcggtgc tggagcgcgc gcagcggctg gaagtgcgag agcgaactga ccatggcgcg
     1621 gcacattgca tcgcggaatt cgagggcggc catcgaaccg tggaagggcg gggcgactcg
     1681 ttgcgcgtcg cgctgttgcg ggcgttcgtc gaaagccgct tcggtgatcg cgtggacgat
     1741 gtgttgcgcg aggcgcaggc cgtcactgga gcgcgtgcgg aaccaatcgg cgaggaattg
     1801 gcgggaggtt cattcgagga tgtgcccagt cccgacgggc aaatcggtga tattcagtcg
     1861 gcaccgcgct agttgctgtg accctgccaa ggccttctgg cttggtgggg tttgtcctga
     1921 ccggcgcgct gaagcaaaac ggttacgcaa aaacaaaggg ccttccgatc ggaaggccct
     1981 tcatagatgg tgcggctggc aggattcgaa cccacgaccc cttggttcgt agccaagtac
     2041 tctatccaac tgagctacag ccgcacgcta aacaggtgat actgcgatga aactgggggt
     2101 aaaacatagg gcctccccgg aggaaggccc tcgaataagt ggtgcggctg gcaggattcg
     2161 aacccacgac cccttggttc gtagccaagt actctatcca actgagctac agccgcacgc
     2221 agaagcgaaa ttatagcaaa cttctaaaaa aagggaagca ctgacgtgga cttcgttgcc
     2281 gaacctgcag cagcgatacg atcggtacgg tacgaagcca tgctgaattg tggcgtacgg
     2341 tgtgccgttc gcgctgtgac gtcttcatag cggctgcgcc gatcgtgtac gcttgttaat
     2401 cgattcaatg aacgttgtgg ttggcatcat gaacaaagcc tttgtcaaag agtcgggcga
     2461 cgacgccgac gacgatctcg atatcgcgca gccggacgtg ccggccggcg caaagaacta
     2521 cattacgccc tccggacaca agcgtctgcg tgacgagctg ctgcatttga tcgacgaaga
     2581 gcggcctgaa gtggttaagc tcgtctcgtg ggccgcgtcg aatggggacc gctcggagaa
     2641 cggcgactac atctacggta agcgccgcct gcgggagatc gatcggcgca ttcgtttcct
     2701 gacgaagcga ctcgatctgg ccgaagtggt cgacagcagc aaacaggaga acctcgacca
     2761 ggtgtttttt ggggccacag tcgactacgc taccgctgac ggggagacgc atacggtcac
     2821 gatagtgggg atcgagtcgc tt
//
LOCUS       DATASH010000012         2384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4748830,
            whole genome shotgun sequence.
ACCESSION   DATASH010000012 DATASH010000000
VERSION     DATASH010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4748830"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1226
                     /locus_tag="VHV99_00285"
     CDS             <1..1226
                     /locus_tag="VHV99_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HEX3633937.1"
                     /translation="GFGASPAIPMPTTTLIQMVNIAPVEERPAGFKPTAPEGFAVTEF
                     ASGLSHPRWLYTLPNGDVLVAESDAPKEHDEGSGVFGWIRQQIMKRAGAGVTSPDRIV
                     LLRDANGSGVAQKQTVFLSGLHSPFGMALVGNALYVADTDALLRFDYTTGSTQIASPG
                     RKVADLPAGPINHHWTKNVLADRSGKHLYITVGSNSNAGENGVDAESDRARILEFDVD
                     TGRLQPYATGLRNANGLSWQPDSGALWTAVNERDDLGNNLVPDYMTAVKEGAFYGFPY
                     SYYGQHVDTRVKPQRPDLVATAIVPDYALGNHTASLGLVFYDRTLFPAHYWGGAFVGQ
                     HGSWNRKPRTGYKVIFVPFVDGKPAGVPEDFLTGFLTADGHAVGRPVGVALDARGALL
                     VADDVGNAVWRVAPR"
     gene            complement(1261..2181)
                     /locus_tag="VHV99_00290"
     CDS             complement(1261..2181)
                     /locus_tag="VHV99_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HEX3633938.1"
                     /translation="MSESKYVERPSRANTVAPLAGEELPIGEPLPWRVVDEDGSVLLR
                     AGTILCREAQRNFLLSHFHPQRGGAAEFQEKSQNAAPQPVTLDDMHLSIGALLGMRSQ
                     IGMGRAMHASRVIGFSPSHTLFVTPPMVGGQPLMLMPGENVEIVAVTTQAVFWFVCTI
                     EAVCTHPFDYLVLSAPGNIRQLRERKAVRVRTHLAVRHGIDLTAQALDGMGVGCDLSV
                     FGMSLATTAPIGRIGERVSVAFQLTTRSVDTEFTTVAVIRNVQTGTNPTALCTHGLEF
                     EGLSSEQQLALKGFVFDKQDAISYWAAHAA"
BASE COUNT          441 a          729 c          745 g          469 t
ORIGIN      
        1 ccggattcgg cgcttcgccc gccataccga tgccaacgac gacgttgatt cagatggtta
       61 acatcgcgcc agtcgaagag cggccggccg gcttcaaacc cacggctcct gaaggcttcg
      121 cagtcaccga gttcgctagc ggtctgtcac acccacgctg gttgtacacg ttgcctaacg
      181 gtgacgttct ggtggcggaa agcgatgccc ccaaagaaca tgacgaggga agtggtgtat
      241 tcggctggat cagacagcag atcatgaagc gcgcaggcgc gggtgtgacc agtcccgatc
      301 gcatcgttct gttgcgtgac gcgaatggca gtggggtcgc ccagaagcaa accgtatttc
      361 tcagcggtct gcactcgcct ttcggaatgg cgcttgtcgg caacgcgctc tatgtggccg
      421 atacggacgc cttgctgcgt ttcgactaca cgacgggcag cacgcagatc gccagccctg
      481 ggcgcaaggt cgccgatttg cccgccggtc cgatcaatca ccattggacg aagaatgttc
      541 tcgcggaccg ctccggcaag catctctaca tcacggtcgg ctcaaacagc aatgcgggag
      601 agaacggcgt cgacgctgaa tcggatcgcg cacgaatact tgagtttgac gtggatacag
      661 ggcgcttgca gccgtatgcg acaggattgc gcaatgccaa cggcctctcg tggcaaccgg
      721 acagcggggc gctgtggacg gcggtcaacg agcgggacga tctgggcaac aacctcgtcc
      781 cggactatat gaccgcggtg aaggagggcg cgttctacgg ttttccttac agctattacg
      841 gccagcatgt cgacacgcga gtcaaacctc aacgccccga tctcgttgcg acggccatcg
      901 tcccggacta tgcgctcgga aatcatacgg cttcgctagg tctggtcttc tacgaccgga
      961 cgctgtttcc ggcgcactat tggggcggcg cattcgtcgg tcagcatggg tcatggaatc
     1021 gcaagccgcg cacaggttac aaagtgatat tcgtgccgtt tgtcgatggg aagcctgcgg
     1081 gcgttcctga agattttttg acgggctttc tgactgcgga cggccatgcc gtggggcgtc
     1141 ctgttggcgt cgcgctggat gcgcgcggcg cccttctcgt ggcggacgac gtgggcaatg
     1201 cggtctggcg agtcgcgccg cgatagccac gtcaaggaag cggttccgaa gcaggacgcg
     1261 ctaagccgcg tgcgcggccc agtacgagat cgcgtcctgc ttgtcgaaca cgaagccttt
     1321 cagcgcgagc tgctgctccg aactcaagcc ttcgaattcg aggccgtgcg tgcagagcgc
     1381 tgttggattc gtgcccgtct gcacgttgcg gatgaccgcg acggtcgtga attcagtgtc
     1441 gacacttcgc gtggtgagtt gaaacgccac gctcacacgc tcgccgatgc gcccgatcgg
     1501 cgcggtcgtc gcgagcgaca tgccgaacac gctcagatcg cagccgactc ccattccatc
     1561 cagcgcctga gccgtcaaat cgattccatg acgcacggcg agatgcgtgc gcacgcgcac
     1621 cgctttacgc tcccgcaact gccggatatt gcccggcgcc gatagcacca ggtagtcgaa
     1681 tggatgcgtg caaaccgcct cgatcgtaca cacgaaccag aacaccgcct gggtggtgac
     1741 cgcgacgatc tcgacgttct cgccgggcat cagcatcaaa ggctgaccgc cgaccatggg
     1801 cggtgtgacg aaaagcgtgt ggctcggact gaacccgatg acccggctcg catgcatcgc
     1861 ccggcccatg ccgatctggc tgcgcatgcc gagcagcgcg ccgatgctca ggtgcatgtc
     1921 gtcgagcgtg actggctgcg gcgcggcgtt ttgcgacttc tcctggaact cggctgcgcc
     1981 gccgcgttgg ggatggaagt gcgacaggag gaagttgcgc tgtgcttcgc ggcacaagat
     2041 ggtgcccgca cgaagcagca cgctgccgtc ctcgtccaca acgcgccacg gaagcggctc
     2101 cccgattggt aattcttcgc cggccaacgg tgcgactgtg ttcgcgcggc ttggccgttc
     2161 gacatacttg ctctcagaca ttgctcaccc gttattctgt ttaagaggct tattttgacc
     2221 gctatatttt ttatacttat cttaccggcg gaattatttt ccgtcaaagc cgggccaacg
     2281 accctcgtcg acgtgagcgt tcccggtcgt cgtggacgtg agcaggggcg tgccgcccag
     2341 gttcgatgcg ggccgacgat gcccagccgc gcgatgcgcc ggta
//
LOCUS       DATASH010000013         4106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2177447,
            whole genome shotgun sequence.
ACCESSION   DATASH010000013 DATASH010000000
VERSION     DATASH010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2177447"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..2548
                     /gene="pheT"
                     /locus_tag="VHV99_00295"
     CDS             113..2548
                     /gene="pheT"
                     /locus_tag="VHV99_00295"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010115709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX3633939.1"
                     /translation="MQFPESWLRTFVDPQLTTDELSHALTMAGLEVEDLRPAAPPTSK
                     IVVGQVLEVVKHPDADKLNVCQVNAGTGATLNIVCGAPNVAPGIKVPVALVGAQLPPA
                     EEGGTPFAIKLSKLRGVESQGMLCSARELKLSEDHSGLMILPEDTPIGQDIRETLNLD
                     DTVFEIKLTPNKADCLSVFGVARETSAITGASLRPLEIKPVEVKLNETLPVKISAPDL
                     CGRFSGRVIRGVNARAKSPQWMVQRLERSGQRSISALVDISNYVMLELGRPSHVFDLD
                     KIHGGMDVRWGRKGETLKLLNGNTVELDETVGVIADEQHIESLAGIMGGDSTAVTLDT
                     TNIYLEAAFWWPDSIRGRSRKYNFSTDAGHRFERGVDYATTVEHIERITQLILDICGG
                     EAGPVDDQIVNVPQREPVKMRVSRANRIIGIKIDADEIAQIFARLGLTFEREGDTFSV
                     MPPSYRFDIEIEEDLIEEVARIYGFEKIPARPPVATSEMLPTNETQRSIHVIRHALAA
                     RDYAETVNFSFVDAEWEQDFAGNDKPVRLLNPIASQLSVMRTTLFGSLINVLRTNLNR
                     RAADRVRVFEAGRVFLHDPSIKAGELTVEGFAQPKMIGGLAYGPALEEQWGAPTRAVD
                     YYDVKGDIEAVLAPAVARFVKAEHPALHPGRSARIELNDRAVGWIGELHPRWMQKYDL
                     PHAPILFEIEAEALMQRVLPIPSDVSRFPPVRRDIAVVVDQKIEAQALLDELRKAQSE
                     EACKTVQKVALFDEFRPKSNTSGGLGAHEKSLAFRVTLQDTGGTLQDETVDLAIQTLV
                     ERLARVYGARLRG"
     gene            2618..3028
                     /locus_tag="VHV99_00300"
     CDS             2618..3028
                     /locus_tag="VHV99_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011851524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEX3633940.1"
                     /translation="MNEMNSSDFEALLTAQRSAMIREIPTSPAAVSTETPTLTKAELA
                     ELLFDNVGLNKREAKDMVEAFFEVIRDALESGDSVKLSGFGNFQLRDKPQRPGRNPKT
                     GEAIPIAARRVVTFHASQKLKALVESGAEASFAR"
     gene            3075..3479
                     /locus_tag="VHV99_00305"
     CDS             3075..3479
                     /locus_tag="VHV99_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX3633941.1"
                     /translation="MTATIEKVVLPPIPAKRYFTIGEVSELCGVKPHVLRYWEQEFTQ
                     LRPVKRRGNRRYYQHHEVLLIRRIRELLYEQGFTINGARNRLDSHGGGAHGAPAEVVE
                     EGEGAAAPTAAAAVDVEQLRKELLHVIDLLGH"
     gene            3543..3619
                     /locus_tag="VHV99_00310"
     tRNA            3543..3619
                     /locus_tag="VHV99_00310"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3577..3579,aa:Pro,seq:ggg)
     gene            3716..>4106
                     /locus_tag="VHV99_00315"
     CDS             3716..>4106
                     /locus_tag="VHV99_00315"
                     /inference="COORDINATES: protein motif:HMM:NF042623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6538 domain-containing protein"
                     /protein_id="HEX3633942.1"
                     /translation="MTNYLLKRGSLYHFRRKVPLKLQAYFGRKEINKALGTSDPNEAK
                     VLCREEAVRHDLAFAEAYRAIESEKAKASARATWHAALEHVCADQEEEFERAQAEHNE
                     EAAALFYQTEEQDIAEHDRHEQEHDALV"
BASE COUNT          876 a         1169 c         1272 g          789 t
ORIGIN      
        1 gcgcggcact gcacgcagtg cgtgccgcca gcagcgtccc cggcagtgcc cagcgaatca
       61 atgggccgcc tgccggacct ggacccaacc tgatcagaac gtacacagaa ccatgcaatt
      121 cccggaatcc tggctgagaa cctttgtcga tccgcaactg acgactgatg aactgtcgca
      181 cgcgttgacg atggcgggtc tcgaagtcga agacctgcgg ccggccgcgc cgccgacctc
      241 gaagatcgtc gtcggccagg tgctggaggt tgtcaagcac ccggacgcag ataagctcaa
      301 cgtgtgtcag gtgaacgccg gcacgggcgc gacgctgaac atcgtgtgcg gtgcgccgaa
      361 tgtcgcgccg ggcatcaagg tgccggtcgc gttggttggc gcgcaactgc cgccggccga
      421 agagggcggc acgccgttcg cgatcaagct ctcgaagctg cgcggcgtgg aaagtcaggg
      481 catgttgtgc tcggcacgcg aactgaagct ctcggaagat catagcggcc tgatgatcct
      541 gccggaagat acgccgatcg gccaggacat ccgcgaaacg ctcaatctcg acgacacggt
      601 tttcgaaatc aagctgacac cgaacaaggc ggactgcctg tcggtattcg gcgtggcgcg
      661 cgagacgtcg gccatcaccg gtgcgtcgct gcgtccgctt gagatcaagc cggtcgaagt
      721 gaagctcaac gaaacgttgc ccgtcaaaat ttcggcgccc gatctgtgcg gccgcttttc
      781 gggccgcgtg attcgcggcg tgaatgcgcg cgcgaagtcg ccgcaatgga tggtgcagcg
      841 tctggagcgt tcgggtcagc gcagcatttc cgcgctcgtc gatatctcga actatgtgat
      901 gcttgaactg ggccgtccgt cgcacgtgtt cgatctggac aagatccatg gcggcatgga
      961 tgtgcgttgg ggccgcaagg gcgaaacgct caagctgctg aacggcaaca ccgtcgaact
     1021 cgatgaaacc gtcggcgtga tcgccgatga acaacacatc gaaagcctcg ctggcatcat
     1081 gggtggcgac agcacggccg tcacgctcga caccaccaac atctatctcg aagccgcgtt
     1141 ctggtggccc gacagcattc gcggccgctc gcgcaaatac aacttctcga ccgatgcggg
     1201 tcatcgtttc gaacgcggcg tcgattacgc gaccaccgtc gaacacatcg aacgcatcac
     1261 gcaactgatc ctcgatatct gcggcggcga agccggcccg gtcgacgatc agatcgtcaa
     1321 cgtaccgcag cgcgagccgg tgaagatgcg ggtctcgcgc gcgaaccgca ttatcggcat
     1381 caagatcgat gcggacgaaa tcgcgcagat cttcgcgcgc cttggcctta cgttcgaacg
     1441 cgagggtgat acgttctcgg tgatgccgcc gtcgtaccgc ttcgatatcg aaatcgaaga
     1501 agatctgatc gaagaggtcg cgcgtatcta cggtttcgag aagatccccg cacgtccgcc
     1561 ggtcgcgacc agcgaaatgc tgccgaccaa cgaaacgcag cgctcgattc acgtgatccg
     1621 tcacgcgctc gccgcgcgcg attacgcgga aacggtcaac ttcagtttcg tcgacgccga
     1681 gtgggagcag gacttcgccg gcaacgacaa gccggtacgt ctgctgaatc cgattgcaag
     1741 ccagttgtcg gtaatgcgca ccacgctgtt cggcagcctg atcaacgtgc tgcgtaccaa
     1801 cctgaaccgt cgcgcggctg accgcgtacg cgtgttcgaa gccggccgcg tatttttgca
     1861 cgacccgtcg atcaaggccg gcgagctgac ggtcgaaggt ttcgcccagc cgaagatgat
     1921 cggcggcctc gcttacggtc ccgcgctcga agagcagtgg ggcgcaccga cgcgcgcagt
     1981 cgactactac gacgtgaagg gcgacatcga agccgtgctc gcgccggccg tggcacgctt
     2041 cgtgaaagcg gagcatccgg ctttgcatcc gggccgcagc gcgcgtattg aactgaatga
     2101 ccgcgcagtg ggctggattg gcgaattgca tccgcgctgg atgcaaaaat atgatttgcc
     2161 gcatgcgcct attctgtttg aaatcgaagc ggaagcatta atgcagcgcg tattgccgat
     2221 tccgtcggat gtgtccagat tcccaccggt gcgtcgcgat attgcggtgg tcgtcgatca
     2281 gaaaatcgag gcgcaggcgc tgctggacga gcttcggaag gcgcagtccg aagaggcttg
     2341 caagactgtc cagaaggttg cgcttttcga cgaattccgt ccaaaatcaa acacttccgg
     2401 tggtctgggt gctcacgaga aaagccttgc gttccgtgtg accttgcaag atactggcgg
     2461 gacccttcag gatgaaacgg tcgatctggc cattcaaact ctggtggaac gtctggctcg
     2521 agtatatggc gcacggttgc gtggataacc cgcatttaac attcgcacgg cattttttcg
     2581 caacctccgt ttctgcttgg cgcgccattt gatagatatg aatgaaatga actcgagtga
     2641 tttcgaagcc cttcttacgg cgcaacgcag cgccatgatt cgcgaaattc cgacatcacc
     2701 cgcggccgtt tccaccgaaa cgccgacgct gaccaaggct gaacttgccg agttgctgtt
     2761 cgacaatgtc gggctcaaca agcgggaagc gaaagacatg gtcgaagcgt tcttcgaggt
     2821 gatccgcgat gcgctggaga gtggcgatag cgtgaagctg tcggggttcg gcaactttca
     2881 gttgcgcgac aaaccccagc gtcccggcag aaacccgaag accggcgagg cgattccgat
     2941 tgccgcgcgt cgcgttgtga cgttccatgc aagtcaaaag ctgaaagcgc tggtcgagag
     3001 cggcgctgaa gcgagcttcg cgcgctgatc gattggcgcg ttcctgcgca acccaccacg
     3061 acggctaact gacgatgaca gcgacgatcg aaaaagtcgt cctgcctccg attccggcga
     3121 agcgctactt cacgattggt gaggtcagcg aactatgcgg cgtgaaaccg cacgtgctgc
     3181 gctattggga acaggagttc acgcagttga ggccggtcaa gcggcgcggc aatcgccggt
     3241 actaccagca tcatgaggtg ctgttgatcc ggcggattcg tgagttgctg tatgagcagg
     3301 gcttcacgat caacggagcg cgtaatcggc tcgattcgca tggcggtggc gcgcacggtg
     3361 cgccggctga ggtggtcgaa gaaggcgagg gcgcagcggc gccgacagca gcggctgcgg
     3421 tagatgtgga gcagttgcgc aaggaattac tgcatgtgat cgatttgttg ggccactagt
     3481 ttgctttgtt ggcgtagggc agttctaatc gattgaagtg tctgttataa tttttagctg
     3541 ttcggggcgt agcgcagcct ggtagcgtac ctgcatgggg tgcaggtggt cggaggttca
     3601 aatcctctcg ccccgaccaa tataacgtgt tgattgcatt cgttaatcaa ccaaatcaat
     3661 ggcttacgag gctagtgtgg ccggtgtaac atacaccccg cactcttcgt aagccatgac
     3721 caactatctt ctcaagcgcg gatcgctgta ccacttccgg cgcaaagtcc ctctgaagct
     3781 ccaagcctac ttcggtcgta aagaaatcaa taaggccctt ggcacgtctg atccaaacga
     3841 agcaaaggta ctttgccgcg aggaagccgt gaggcatgac ttggcctttg cggaagccta
     3901 tcgcgctatc gaatcggaaa aagctaaggc aagtgcgcgg gccacatggc acgcagcgct
     3961 agagcatgtg tgcgcagacc aagaggaaga attcgagcga gcgcaagcag agcacaacga
     4021 ggaggcggcg gcgcttttct accagacgga ggagcaggac attgcggaac acgaccgcca
     4081 tgagcaggag catgatgcgc tagtgt
//
LOCUS       DATASH010000014        12590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3166731,
            whole genome shotgun sequence.
ACCESSION   DATASH010000014 DATASH010000000
VERSION     DATASH010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 12590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12590
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3166731"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /gene="recA"
                     /locus_tag="VHV99_00320"
     CDS             complement(<1..987)
                     /gene="recA"
                     /locus_tag="VHV99_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEX3633943.1"
                     /translation="MEESKKGSAGLTAEKSKALAAALAQIEKQFGKGSVMRLGAGEVV
                     EDIQVVSTGSLGLDIALGVGGLPRGRVVEIYGPESSGKTTLTLQVIAEMQKIGGTAAF
                     IDAEHALDIQYAGKLGVNVNDLLVSQPDTGEQALEIADALVRSGSIDMIVIDSVAALV
                     PKAEIEGEMGDSLPGLQARLMSQALRKLTGTIKRTNCLVIFINQIRMKIGVMFGNPET
                     TTGGNALKFYASVRLDIRRIGSIKKNDEVIGNETRVKVVKNKVSPPFREAIFDILYGE
                     GISRQGEIIDLGVQAKLVDKAGAWYSYNGERIGQGKDNAREFLRENPDMAREI"
     gene            1230..2009
                     /locus_tag="VHV99_00325"
     CDS             1230..2009
                     /locus_tag="VHV99_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3633944.1"
                     /translation="MRILIAEDDSILADGLVRSLRQSAYAVDHVKSGVEADTALSMQT
                     FDLLILDLGLPRMSGLEVLRRLRARNSTVPVLILTAADSVDERVKGLDLGADDYMAKP
                     FALNELEARVRALTRRGAGGGPTVVRHGSLSFDQVGRIAYVNEQVIDLSARELGLLEV
                     LLQRIGRLVSKEQLVDHLCEWGEEVSNNAIEVYVHRLRKKIEPSGVRIITVRGLGYCL
                     EKAPLPANVNANANVSANAPTEPEPPTSPSPAAMPASHHYK"
     gene            2019..3557
                     /locus_tag="VHV99_00330"
     CDS             2019..3557
                     /locus_tag="VHV99_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HEX3633945.1"
                     /translation="MSARADRATAHAADLDEARDERYANPFAPPDETEAAAEARPRSL
                     FGEILDWMLAPLLLLWPMSLAVTYLVAKSIANGPFDRALETDAYVLARQIHPVNGVAE
                     LSLPDSTRDFLRADNVDSVFYQVLGTRGELVGGERDMPLPHEEDRPPPGLVEFRDDML
                     RGNDIRVAYTTVEFPQTPGAQPVLVQVAETLDKRSQLANDIIKGVILPQFVILPLAIL
                     LVWFGLSRGLAPLHALQAHIRARRPDDLSPLEARRAPPEIEPLVTSFNDLLTRLEQNM
                     ELQKRFIAYAAHQMKTPLAGLRTQAELALRQDASAEVHRSLEQIATSSEHAARLVTQL
                     LALARAENRMSGQIFTPVEVTEVARSAVRDWVQAALAKQMDLGYEAPEEPVEVDGNPV
                     MLREMLSNLIDNAIRYTPAGGRITVRVRHDATARRVHLEVEDTGLGIPVAERSRVVER
                     FYRILGREGDGSGLGLAIVREIATMHGGEMTIDDNVYQTSPRLAGTLVRVSLQVLERA
                     RDLP"
     gene            3788..5446
                     /locus_tag="VHV99_00335"
     CDS             3788..5446
                     /locus_tag="VHV99_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011546128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3633946.1"
                     /translation="MATVGGQISHMPMTHEEKRVIFASSLGTVFEWYDFYLAGSLAAF
                     ISKSFFSGVNPTAAFIFTLLSFAAGFAVRPFGAIVFGRLGDMVGRKYTFLVTIVIMGL
                     STFLVGFLPGYASIGIASPVIFIAMRMLQGLALGGEYGGAATYVAEHAPAGRRGFYTA
                     WIQTTATLGLFLSLLVILGVRTAMGEDAFGVWGWRIPFIASIILLGVSVWIRLQLHES
                     PVFERIKAEGKTSKAPLTEAFGQWKNLKIVILALIGLTAGQAVVWYTGQFYTLFFLTQ
                     TLKVDGATANILIAIALLIGTPFFLFFGSLSDRIGRKPIIMAGLLIAACTYFPLFKAL
                     THYTNPALEAATAKAPIVVIANPDECSFQFNPVGTSKFTSSCDIAKSALSKSGLNYEN
                     VAAPAGTLAEIKVGDTVINTYDGKAADAKDQGKVFDKTLATTLKTAGYPPKADPSQIN
                     WPMSVVILTIMMIYVTMVYGPIAAMLVEMFPTRIRYTSMSLPYHIGNGWFGGFLPATA
                     FAIVAAKGNIYSGLWYPIVIALITLVIGVLFVKETKDSDIYAKD"
     gene            5533..5609
                     /locus_tag="VHV99_00340"
     tRNA            5533..5609
                     /locus_tag="VHV99_00340"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5567..5569,aa:Met,seq:cat)
     gene            5696..6046
                     /locus_tag="VHV99_00345"
     CDS             5696..6046
                     /locus_tag="VHV99_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3633947.1"
                     /translation="MTFRTAALAVATASIALLAGASPLTAAAQTPPDSQWRTSPITLS
                     TLLQDGYKIVAVVNDAHGNAGSADTIFVQRDQSAFKCIDPQQPDPKAKQPVTPAACFE
                     LVQPSGAVAGSEAK"
     gene            complement(6163..7650)
                     /locus_tag="VHV99_00350"
     CDS             complement(6163..7650)
                     /locus_tag="VHV99_00350"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3633948.1"
                     /translation="MDWGDPAKGEITIAVTRLAVKDPARRMGSLLFNPGGPGGDGRLA
                     NLTFYRQLAGVDATVSERQLLDAYDLIGFSPRGMGSSTQLTCGSTQRLSTVWWGSDVS
                     ASTVDALLRNAELKSKACQNNPLTSYINTGQIARDMDLIRAISGDAKLNYMGHSYGTW
                     LGAWYAGLFPERTGRMVLSANLDFSARTMAEAGTLQQPPAIQRIADEIVGPYAARHDA
                     HFNLGSNPAATRQLFAALPPRLQAAASSALDQSRVFALAGYIDEGASVFVAAKGVQQI
                     LEQNPGSTEDQILALARHHAFSRHRLANQMALQIATKTVIPKYFDAEQPSPFKDDPLH
                     MTTEEAVDLAVMCNDTPAFSTSRQLWIDKHREYAQRYPLGSGTLGGSVLNFSCLFWKP
                     PQPAVLKPTIDEVARAPSILMIQAQFDGFTAAEGALRMFDKLPNASLLYIKGEHSHVE
                     YPVGNPCVDEPVARYLLTGTLPPRRVDCEDSRKLWLDAGNELEPV"
     gene            complement(8163..8606)
                     /locus_tag="VHV99_00355"
     CDS             complement(8163..8606)
                     /locus_tag="VHV99_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX3633949.1"
                     /translation="MNTQLEERFGFLISDVGRLTGKRFDDLAKSSVDLTRAQCRVLAN
                     LAHYGDVNQARLADLLEVAPISAGRLLDRMEEGGWIERTANPQDRRERQVRMTPKAER
                     TLGKARKVGDEVALEALNGFTDEEAKQLIALLQRVRGNLSRLVDR"
     gene            8765..10303
                     /locus_tag="VHV99_00360"
     CDS             8765..10303
                     /locus_tag="VHV99_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX3633950.1"
                     /translation="MSSDSPPTSAAAPLNRPMITVSIMLATLIQTLDSTIANVALPHM
                     QGTLSASQDEITWVLTSYIVAAAIATPLTGWLSDRLSVKRLLIFAIGGFTMASALCGL
                     SETLTQIVASRLLQGIFGASLVPLSQSILLDINPREKQGQAMAVWGMGVMVGPILGPT
                     LGGWLTDSYNWRWVFFINVPIGAFALFGVATFLPAREPKHDAKFDAFGFATLGLSIGA
                     LQAMLDRGEQLDWFGSHEIVIEALVAAISFAFFLVHTATVGKKSFFKYELLKDPNFAT
                     GTFFIFVIGAVMYATRALLPPMLQNLMNYPVATTGLVTAPSGAGTMVAMLFAGRLLKR
                     VDARLLLLAGFLISAVALWQMMHYTIVLSASDIVWPGVIQGFGLGLVFVPLSALTFST
                     LTPALRADGTATYSLMRNIGSSIGISIVQTLMTRNTQVSHADLVANITPFNPAIQPML
                     NSGSNYDMAALNASITQQASMIAYLNDFKLMFMATLFVIPLLLLIRPARQAPEASVAH
                     AAMD"
     gene            complement(10352..11065)
                     /locus_tag="VHV99_00365"
     CDS             complement(10352..11065)
                     /locus_tag="VHV99_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEX3633951.1"
                     /translation="MSIAAQGRREKAIQHDLHGRVAHLLATAILRGDYAPDSILPREA
                     ELMDTFGVSRTVLREALRTLTSKGLIESRPRVGTRVRPKIAWNLLDVDVLDWYSRVAE
                     PMAFALKLQEMREMIEPYAAGLAAASHTDDTFNALEVAHASMVGARNVDEWVRADLQF
                     HLSVLTACSNELLIPLGTLIERTLEAQLRLNAKRADVFNASLAEHTAVFEAIRDRDAA
                     AAREAMAGLLGVTRGRIEG"
     gene            complement(11118..11276)
                     /locus_tag="VHV99_00370"
     CDS             complement(11118..11276)
                     /locus_tag="VHV99_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3633952.1"
                     /translation="METGGWPKRYSPGDRNDHDIDHDFDIDIDIDHDDVAAERRPELI
                     HAGTAPKP"
     gene            11379..12527
                     /gene="dgoD"
                     /locus_tag="VHV99_00375"
     CDS             11379..12527
                     /gene="dgoD"
                     /locus_tag="VHV99_00375"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HEX3633953.1"
                     /translation="MKITRLETFIVPPRWCFLKIETDEGIIGWGKPVVEGRAHTVAAA
                     VEELSDYLIGKDPLLIEDHWQVMYRAGFYRGGPITMSAIAGVDQALWDIKGKHHGVPI
                     HALLGGQVRDKIKVYSWIGGDRPGDVANNARAVVERGFKAVKMNGSEELQIIDTFDKV
                     QGVINNVAAVREAVGPNIGIGVDFHGRVHKPMAKVLAKELDPYKLLFIEEPVLSENAE
                     ALRDIVNQTSTPIALGERLYSRWDFKHILSGGYVDIIQPDASHAGWITECRKIASMAE
                     AYDVALALHCPLGPIALATCLQIDAVSYNAFIQEQSLGIHYNQGNDLLDYIKNPEVFK
                     YEDGFVSIPQGPGLGIEVNEEKVREMAKVGHRWRNPVWRHTDGSVAEW"
BASE COUNT         2314 a         3994 c         3796 g         2486 t
ORIGIN      
        1 gatctcgcgg gccatatccg gattctcacg caggaattca cgtgcgttgt ccttgccctg
       61 accaatccgt tcgccgttgt agctgtacca ggcgccggcc ttgtcgacga gtttggcctg
      121 cacgcccagg tcgatgattt cgccctgacg cgaaatacct tcgccgtaca gaatgtcgaa
      181 aatcgcttcc cggaacggtg gcgacacctt gttcttcacg accttcacgc gcgtttcatt
      241 gccgatcact tcgtcgttct tcttgatcga gccgatccgg cgaatgtcca gacgcaccga
      301 cgcgtagaac ttcagcgcgt taccaccggt ggtggtttcc gggttgccga acatcacgcc
      361 gatcttcata cggatctggt tgatgaagat cacgaggcag ttcgtgcgct tgatcgtgcc
      421 ggtgagcttg cgcagcgctt gcgacatcag acgcgcttgc agacccggca gcgagtcgcc
      481 catttcgcct tcgatttcgg ccttcggcac gagtgccgcg accgagtcga tcacgatcat
      541 gtcgatcgaa cctgagcgca ccagtgcgtc ggcgatttcg agcgcctgtt caccggtatc
      601 cggctgcgaa accagcaggt cgttcacgtt cacgccgagc ttgcccgcgt actggatgtc
      661 cagcgcgtgt tccgcgtcga taaacgccgc cgtgccgccg atcttctgca tttcggcgat
      721 gacttgcagc gtcagcgtgg ttttaccgga tgattccgga ccataaattt cgaccacacg
      781 gccacgcggc aaaccgccga cgcccagcgc gatgtcgagg ccaagcgatc cggttgagac
      841 cacctggatg tcttcgacta cctcacctgc gccgagccgc atgaccgacc ctttgccgaa
      901 ctgtttttcg atctgcgcaa gcgcggcagc gagtgccttg ctcttttcag cagtcagtcc
      961 agccgagcct ttcttgcttt cttccatgaa tcgtcctttg ctatgatgaa cggcgcctga
     1021 ggcagatgtg cagcccacga gttaacctac tcggcaagga cagcacacga aactcagaca
     1081 ctgtataaaa aaacagtagt tttggcaagc gcgattctca tgcgaacgcg catttttacc
     1141 gtcgcgccgc ccaactaccc acaataattt tcggagaccg ctgtgcgcca tggataccca
     1201 cggtgccggc aaggccaggt tggcgcacca tgcgaattct gattgccgaa gacgacagca
     1261 tactcgcgga cggtctggtt cgatcactcc gccaatcggc ctatgccgtc gatcacgtga
     1321 agagcggcgt cgaggccgat accgcccttt cgatgcaaac tttcgacctg ctgatcctcg
     1381 atctgggcct gccgcgcatg tcggggctcg aggtgctgcg ccgcctgcgc gcccgcaact
     1441 cgaccgtgcc ggtgttgatt ctcacggccg cggacagcgt cgacgaacgc gtcaagggac
     1501 tcgatctcgg cgccgacgat tacatggcca agcccttcgc gctcaatgaa ctcgaggcgc
     1561 gcgtgcgcgc gctgacccgg cgcggcgcgg gcggcggccc gacggtggtg cggcacggtt
     1621 cgctgtcgtt cgatcaggtc ggccggatcg cctatgtcaa cgagcaggtg atcgacctgt
     1681 cggcacgcga actcggtttg ctcgaagtcc tgctgcaacg gatcggccgg ctggtgtcga
     1741 aggaacaact cgtcgaccat ttgtgcgaat ggggcgagga agtcagcaat aacgcgatcg
     1801 aagtctatgt gcaccggcta cgcaagaaga tcgagccgag cggtgtgcgt atcatcaccg
     1861 tgcgggggct cgggtattgc ctcgaaaaag cccccctgcc cgcgaacgtg aatgcgaacg
     1921 caaatgtgag cgccaacgcc cccaccgagc ctgagccgcc cacctcgccc tcgccggcag
     1981 ccatgccggc gagccatcac tacaaataga gcacgcccat gtccgcacgc gcagaccgcg
     2041 ccacggcgca cgcggcggac ctcgacgagg cgcgcgacga gcgctacgcc aatccgttcg
     2101 ccccgcccga cgagaccgaa gccgccgccg aagcgcgccc gcgctcgctg ttcggcgaga
     2161 ttctcgactg gatgctggcg ccgctactgc tgctttggcc gatgagcctc gcggtcacct
     2221 acctggtcgc caagtcgatc gccaacggcc cgttcgaccg cgcgctcgaa accgacgcct
     2281 atgtgctcgc ccgccagatc catccggtca acggcgtggc ggagctctcg ctgcccgact
     2341 ccacccgcga ctttctgcgt gccgacaacg tcgacagcgt gttctatcag gtgctcggca
     2401 cgcgcggcga actggtgggc ggcgagcgcg acatgccgct gccgcacgag gaagaccggc
     2461 cgccgccggg cctcgtcgaa tttcgtgacg acatgctgcg cggcaacgat atccgcgtcg
     2521 cctatacgac cgtcgagttt ccgcagacgc ccggcgcgca gcctgtgctg gtgcaggttg
     2581 ccgaaacgct cgacaaacgc agccagcttg ccaacgacat catcaaaggc gtgatcctgc
     2641 cgcagtttgt gatcctgccg ctggcgatcc tgctggtctg gttcggcctg tcgcgcggcc
     2701 tcgcgccgct gcatgccctg caggcgcata ttcgcgcacg ccgcccggac gatctgtcgc
     2761 cgctcgaagc ccgccgcgcg ccgccggaaa tcgagccgct ggtgacgtcg ttcaacgatc
     2821 tgctgacgcg cctcgaacag aacatggagt tgcagaagcg ctttatcgcc tacgccgcgc
     2881 accagatgaa aacaccgctt gccggcctgc gcacccaggc tgaactcgcg ctgcgccagg
     2941 acgcctccgc ggaagtccac cgctcgctcg aacagatcgc caccagttcg gagcacgcgg
     3001 cgcgtctggt cacccagttg ctggctctgg cgcgcgcgga aaaccgcatg tcgggccaga
     3061 ttttcacgcc ggtcgaggtg acggaagtcg cccgcagcgc cgtgcgcgac tgggttcaag
     3121 ccgccctcgc caagcagatg gacctcggct acgaagcgcc cgaagagccg gtcgaagtcg
     3181 acggcaatcc ggtcatgctg cgggagatgc tgtcgaatct gatcgacaac gcgatccgtt
     3241 acacgccggc tggcggccgg atcaccgtgc gggtaagaca tgacgcgacg gcgcggcgcg
     3301 tgcatctcga agtggaagac actgggcttg gcattccagt ggccgagcgc tcgcgggtag
     3361 tcgagcgttt ttaccggatt ctcggccgcg aaggcgacgg cagcggcctt gggctcgcga
     3421 tcgtccggga gatcgcgacg atgcacggcg gggagatgac gatcgacgat aacgtctatc
     3481 aaacttcccc acggcttgcc ggaacgcttg tgcgtgtcag cttgcaggtg ctcgaacggg
     3541 cccgggactt accctaagcg cacgaatagc cgcgccttgc ccgccaccac attgagatga
     3601 cttggcatca agtttgataa atttagcgtg aagttgacga tttttgacag aaatctccct
     3661 cgcacaatcg atctgccact ttcacccctt cgcgtcagta cgccgtaagt ttccactcca
     3721 ataatcggtt ccacgggaac gcctttgccg gcggaccgcg tgtacatatc aatattggag
     3781 acgacatatg gctaccgttg gcgggcaaat ctcgcacatg ccgatgacgc acgaagagaa
     3841 gcgggtgatc ttcgcatcgt cgctaggcac ggttttcgag tggtacgact tctatctggc
     3901 cggctcgctc gcggccttca ttagcaagag cttcttctcc ggcgtcaatc cgactgccgc
     3961 cttcatcttc acgctcctca gcttcgccgc tggcttcgcg gtgcggccgt tcggcgcgat
     4021 cgtgttcggg cgcctcggcg atatggtggg gcgcaagtac accttcctcg tcacgattgt
     4081 gatcatgggc ttgtcgacgt tcctggtcgg cttcctgccg ggttatgcat cgatcggcat
     4141 tgcctcgccg gtgatcttca tcgccatgcg gatgctgcaa ggtctcgcgc tcggcggtga
     4201 atatggcggt gcggcaacct atgtcgcgga acatgcgcct gcgggacgcc gcggcttcta
     4261 caccgcatgg atccagacga ccgccaccct cggcctgttc ctgtcgctgc tggtgattct
     4321 cggcgtgcgc acggccatgg gcgaagacgc gttcggcgtt tggggctggc ggattccgtt
     4381 catcgcgtcg atcatcctgc tcggcgtgtc ggtgtggatc cggctgcaac tgcatgaatc
     4441 gccggtattc gagcgcatca aggccgaagg caagacctcg aaggcgccgc tgaccgaagc
     4501 attcggtcaa tggaagaacc tgaagatcgt gatcctcgcg ctgatcggcc tgaccgccgg
     4561 ccaggccgtg gtctggtaca ctggccagtt ctacacgctg ttcttcctga cgcagacgct
     4621 gaaggtggat ggcgcgacgg ccaacatcct gatcgcgatc gcgcttctga tcggcacgcc
     4681 gttcttcctg ttcttcggct cgctgtcgga ccgcattggc cgcaagccga tcatcatggc
     4741 cggcctgctg attgctgcgt gcacgtactt cccgctgttc aaggcgctga ctcactacac
     4801 gaatccggcg ctggaagcgg ctacggcaaa agccccgatc gtcgtgattg cgaatccgga
     4861 cgagtgctcg ttccagttca atccggtcgg cacctcgaag ttcacgagtt cctgcgacat
     4921 cgccaagagc gcactctcga aatctggctt gaactacgag aacgtcgcgg cgccggcggg
     4981 cacgctggcg gagatcaagg tcggcgatac ggtgatcaac acgtatgacg gcaaggccgc
     5041 cgacgccaag gaccagggca aggtgttcga caagactctg gccacgacgc tgaagaccgc
     5101 cggctatccg ccgaaggccg atccgtcgca gatcaactgg ccgatgagcg tggtgatcct
     5161 gacgatcatg atgatctacg tgacgatggt ctatgggccg attgcggcga tgctggtgga
     5221 gatgttcccg actcgcatcc gctatacctc gatgtcgttg ccgtatcaca tcggcaatgg
     5281 ctggttcggc ggcttcctgc cggccaccgc cttcgcgatc gtggcggcca agggcaacat
     5341 ctactcgggg ctgtggtatc cgatcgtgat cgcgctcatc acgctggtga tcggtgtgct
     5401 gttcgtcaag gaaaccaagg actcggatat ttacgcgaag gactgacacg cgtgacggat
     5461 ggggcaattt aaaaaagttg cgcagcgggg gttgacagcc cccggcccta tcctcataat
     5521 ctcgcttctt tcggcgaatt agctcagttg gttagagcga cggaatcata atccgcaggt
     5581 ccggggttcg agtccctgat tcgccaccag ttgcaagaaa aagccgctga tccacaaggt
     5641 cagcggcttt tttgttttgg cttttccaaa ctgcaacttc aaggaggttt tcttgatgac
     5701 gttccgcacc gccgcgctcg ccgtggccac cgcatccatc gcgcttctgg ccggcgcatc
     5761 gccgcttacc gccgccgccc aaacgcctcc cgacagtcag tggcgcacca gtccaattac
     5821 gctttcgacg ttgctgcagg acggctacaa gatcgttgcc gtcgtgaatg atgctcacgg
     5881 caatgccggt tcggccgata cgatctttgt tcaacgcgat cagagcgcat tcaagtgcat
     5941 cgacccgcag cagcccgatc ccaaagcgaa acagccggtc acgccggctg cctgcttcga
     6001 gctggtccag ccgtccggcg cggtggcggg ctccgaggcc aagtagtcgc acgctcgcga
     6061 taaccggcgg cggcccgctt actcgcgggc ccggctcaat gtggcaaccg tcgacaaatc
     6121 cagccacttg cgctgctcac gcccgcgcaa cacggctgta ggtcagaccg gctccaactc
     6181 gttccctgca tcgagccata gcttcctgct gtcctcgcag tccactcgtc gaggcggtag
     6241 tgtgccggtc agcaagtagc gcgcgaccgg ctcatcgaca cacgggttgc cgaccggata
     6301 ttcgacgtgc gaatgctcgc cttttatgta gagcaagctc gcgttcggga gtttgtcgaa
     6361 catgcgcagc gcgccctcag cagccgtgaa gccgtcgaat tgcgcctgaa tcatgaggat
     6421 cgatggcgca cgcgccacct catcgatcgt cggcttgagc accgccggct gcggcggctt
     6481 ccagaacagg caactgaaat tgagcacgct accacccagc gttccactgc ccagcggata
     6541 tcgctgcgcg tattcccggt gtttgtcgat ccatagttgc ctgctagtgc tgaacgcagg
     6601 cgtgtcgttg cacatgactg ccagatccac ggcctcttcg gttgtcatat gcagcgggtc
     6661 gtctttgaaa ggagaaggct gctcagcgtc aaagtacttc gggatgacag tcttcgttgc
     6721 gatctgcagt gccatctggt ttgctagccg atggcgcgaa aacgcgtgat gccgggcgag
     6781 cgccaggatc tgatcctccg tactaccggg gttctgctcg agtatctgct ggacaccttt
     6841 cgccgctacg aatacggacg ccccttcatc gatatagcca gcgagtgcaa atacacgcga
     6901 ttgatcgagt gcggacgaag ccgctgcctg cagcctcggc ggcaacgccg caaacagttg
     6961 ccgtgttgcc gccgggttgc tgccaagatt gaaatgcgcg tcatggcgcg ctgcgtaggg
     7021 cccaactatc tcgtcagcga tgcgctggat cgctggcggc tgctgcaagg taccggcctc
     7081 ggccatggta cgcgcggaga aatccagatt ggcgctcaac accattcttc cggtgcgctc
     7141 aggaaacagc cctgcatacc aggcgccgag ccatgtgcca tacgagtggc ccatgtaatt
     7201 gagtttggcg tcgccggata ttgcccgaat cagatccatg tcccgggcaa tctgaccggt
     7261 gttgatatag gaggtgagcg ggttgttctg gcacgccttc gatttgagtt cggcattgcg
     7321 cagcaaggca tcgacggtcg acgcgctaac gtcgctgccc caccaaacgg tgctgaggcg
     7381 ctgggtcgaa ccgcaggtca attgcgtgct tgaacccatt ccccgcggcg aaaagccgat
     7441 cagatcgtac gcgtcgagca gttgtcgctc agaaaccgtc gcatccacgc cagccaattg
     7501 gcgatagaac gtgaggttcg ccaggcggcc gtctccgccc ggcccgcctg ggttgaacag
     7561 cagcgaaccc atgcgccgcg ccggatcctt cacggcaagc cgcgttactg cgatcgttat
     7621 ctcgccttta gcagggtctc cccagtccat cggtgcgcga agatccgcgc accgaaagcg
     7681 cggagacgct gcgattttct cgtaagccgc aggcaagccg ctcttgtcac acgggagcca
     7741 acccacctgt tgctcaaagt acggtttcag tgccgaagcg atttttgggt tgtgttcgaa
     7801 gcgcggtttc gctgccgaag cgcgcttctg ttgcggtgtc gggtcatctt ccccgccacc
     7861 gccgcagcca gccaaggcag cggcgaagat gatcaacgtc gtttttgccg agatggtttt
     7921 catcactacc tctctccccg accaggtcgg tcggcttgcc gccgtccgca tcgcgcacga
     7981 cagcgtcggg tgattgaacg tcccggctga gcggcaatag cactcggcca gaaggaacgg
     8041 cagcgagtga aaagatcaac tgatacgctg cctgtgaata ttggcaggtc aaggacggcc
     8101 ttcggcgtgg tccaacgccg cagctgcggg taatcacaga cagcagtgga tggttggcgg
     8161 cttcagcgat ccacgaggcg gctgagatta ccgcgtaccc gttgcagcag cgcaatcagc
     8221 tgtttcgctt cttcgtcggt aaaaccgttt agtgcttcga gcgcgacttc atcgcccact
     8281 ttgcgcgcct tgccgagcgt gcgctcggcc tttggcgtca tacgcacctg cctctcgcga
     8341 cgatcctgcg gattggccgt gcgctcgatc cagccaccct cctccatgcg gtccagcaga
     8401 cgtcccgccg aaatcggcgc gacttcgagc aggtccgcga gccgcgcctg attgacgtcg
     8461 ccgtaatggg cgagattggc cagcacgcgg cactgcgcgc gtgtcagatc gaccgatgac
     8521 ttcgcgagat cgtcgaaacg tttgcctgtc aggcggccga cgtcggaaat cagaaagccg
     8581 aagcgttctt caagttgggt gttcatgcgc ggattatacg aaaggctttt gcttgtcatc
     8641 atcggggcgg gtgccgaatc ggtgcgcaac gcgtgcaatt caggctccgc ataattcctg
     8701 atgggcgcgt gcgcttgcgg ccgatatact aagcatgctt actatctcga cgatccgctt
     8761 ccccatgtcc tccgactccc cgccgacgag cgccgccgcc ccgctcaacc ggccgatgat
     8821 cactgtctcg atcatgctcg cgacgctgat ccagacgctc gacagcacca tcgccaacgt
     8881 cgcgctgccg cacatgcagg gcacgttgtc cgcgtcgcag gatgaaatca cgtgggtgct
     8941 gacttcgtat atcgtcgcgg cggcgatcgc cacgccgctg accggctggc tgtccgaccg
     9001 gctgagcgtc aagcggctgc tgatcttcgc gatcggcggc ttcacgatgg cctcggcgct
     9061 gtgcggatta tcggagacgc tgacgcagat cgtcgcgtca cgtttgctgc agggaatttt
     9121 cggggcctcg ctggtgccgc tgtcgcagtc gatcctgctc gacatcaatc cgcgcgaaaa
     9181 gcagggccag gcgatggcgg tatggggcat gggcgtgatg gtcgggccga ttctcgggcc
     9241 gacgcttggc ggctggctca ccgatagcta caactggcgc tgggtgtttt tcatcaacgt
     9301 gccgatcggt gcgttcgcgc tgttcggcgt ggcgaccttc ctgcccgcgc gcgaaccgaa
     9361 gcacgacgcg aaattcgacg cgtttggctt tgccacgctg ggtctctcga tcggcgcact
     9421 gcaggccatg ctcgaccgcg gcgagcaact cgactggttc ggctcgcatg agatcgtcat
     9481 cgaggcgctc gtcgcggcga tcagcttcgc gttcttcctc gtgcataccg ccacggtcgg
     9541 caagaaatcg ttcttcaaat acgagttgct gaaggatcca aacttcgcaa ccggcacgtt
     9601 ctttatcttc gtgatcggcg cggtgatgta tgcgacgcgc gcgctgctgc cgccgatgct
     9661 gcaaaacctg atgaactatc cggtcgcgac cacgggtctc gtcacggcgc cgagcggcgc
     9721 gggaaccatg gtcgcgatgc tgttcgccgg gcggctcttg aagcgggtcg acgcgcggct
     9781 tctactgctc gccggcttcc tgatctccgc ggtcgcgcta tggcagatga tgcattacac
     9841 gatcgtgctg tcggcgtcgg acattgtctg gcccggcgtg atccagggct tcggcctggg
     9901 tctcgtgttc gtgccgctga gcgcgctgac cttctcgacg ctcacgcccg cgttgcgcgc
     9961 ggacggcacc gccacgtaca gcctgatgcg caatatcggc agcagtatcg gcatttcgat
    10021 cgtgcagacg ctgatgacgc gcaacacgca ggtctcgcat gcggacctcg ttgcgaatat
    10081 cacgccgttc aatccggcga tccagccgat gctcaacagc ggctcgaatt acgacatggc
    10141 ggcgctgaac gcgtcgatta cacagcaggc gtcgatgatc gcctatctga acgacttcaa
    10201 gctgatgttc atggccacgc tgttcgtgat tccgttgctg ctgttgatcc gtccggcgcg
    10261 ccaggcgccg gaggcgtcgg tggcacatgc agcgatggat tgagcacgat gattggggtc
    10321 aagcgggagc ggcggcacac tcgtgccacg ctcacccctc gatcctgccg cgcgtcacgc
    10381 ccagcaaccc cgccatcgcc tctcgcgcag ccgctgcatc acggtcacgg atcgcttcga
    10441 ataccgccgt gtgctcggcc agtgacgcgt taaacacatc cgcgcgtttc gcattcaggc
    10501 gtaattgcgc ttccagcgtg cgctcgatca gcgtgccgag cggaatcagc aattcgttgc
    10561 tgcatgccgt cagcacgctc aaatgaaact gcaaatcggc ccgcacccat tcatcgacat
    10621 tgcgcgcccc gaccatcgac gcatgcgcga cctccaatgc attgaaagtg tcgtcggtat
    10681 gcgaggccgc ggcgagaccc gccgcgtacg gctcgatcat ctcgcgcatt tcttgcagct
    10741 tgagcgcgaa cgccatcggc tcggcaacgc gcgagtacca gtcgagcacg tcgacatcga
    10801 gcagattcca cgcgattttt ggccgcacgc gcgtgcccac gcgcggacgc gattcgatca
    10861 gccccttcga cgtcaacgtg cgcagtgcct cgcgtaacac ggttcggctc acgccgaagg
    10921 tgtccattaa ctccgcctcg cgcggcagga tcgagtcggg cgcgtagtcg ccgcgcagaa
    10981 tcgcggtcgc cagcagatgg gcgacgcgcc catgcagatc gtgctgaata gctttctccc
    11041 ggcggccttg cgccgctatt gatatcgaac ctgagaccgg aacggaccgc gattatccgg
    11101 tatctcgacc agccaaccta tggcttcggt gccgttccgg cgtgaataag ctcgggccgg
    11161 cgctcggccg cgacatcgtc gtggtcgatg tcgatgtcga tgtcgaagtc gtggtcgatg
    11221 tcgtggtcgt tgcggtcgcc cggggagtaa cgcttcggcc agccccccgt ctccaactgg
    11281 caaatagtac tattaatact acttaaacgc gcttttgttg tagcatgtga atcctcgaaa
    11341 tattccgcag gcgcccaagc cgcaaggaga cgcacaccat gaaaatcacc aggctcgaaa
    11401 cctttatcgt tccgccacgc tggtgcttcc tcaagatcga aacggacgaa ggcatcatcg
    11461 gctggggcaa gcccgtggtc gaaggccgcg cgcatacggt ggccgccgcc gtcgaggaac
    11521 tgtccgacta tctgatcggc aaagacccgc tgctgatcga agaccactgg caggtcatgt
    11581 atcgcgcggg cttctaccgc ggcggcccga tcacgatgag cgcgatcgcc ggcgtcgacc
    11641 aggcgctgtg ggacatcaag ggtaagcatc atggcgtgcc gattcatgcg ctgctgggcg
    11701 gccaggtgcg cgacaagatc aaggtgtatt cgtggatcgg cggcgaccgt ccgggcgatg
    11761 tcgcgaacaa cgcacgcgcc gtggtcgaac gcggcttcaa ggccgtgaag atgaacggct
    11821 cggaagaatt gcagatcatc gatacgttcg acaaggtgca aggcgtcatc aacaacgtcg
    11881 ccgccgtgcg cgaagcggtc gggccgaata tcggcatcgg cgtggacttt cacggccgcg
    11941 tgcacaagcc catggcaaag gtgctggcca aagaactcga cccgtacaag ctgcttttca
    12001 tcgaagagcc ggtgctatcg gaaaacgcgg aagcattgcg cgacatcgtc aatcagacca
    12061 gcacgccgat cgctctgggc gagcggctgt attcgcgctg ggacttcaag cacatcctgt
    12121 cgggcggcta tgtcgacatc atccagccgg acgcgtcgca cgcgggttgg attaccgagt
    12181 gccgcaagat cgcgtcgatg gccgaggcgt acgacgtcgc gctcgcgctg cactgcccgc
    12241 tcggtccgat cgcgctggcc acctgtctgc agatcgacgc ggtcagctac aacgcgttca
    12301 tccaggagca gagcctgggc attcactaca accagggcaa cgatctgctc gactacatca
    12361 agaatcccga agtgttcaaa tacgaagacg gcttcgtgtc gattccgcaa ggccccggcc
    12421 tcggtatcga agtgaacgaa gagaaagtcc gcgaaatggc gaaggtcggc catcgctggc
    12481 gcaatccggt gtggcgccat acggacggca gcgtcgccga gtggtaagtc tgtgatgaaa
    12541 cgccgcctgc gggcggcgtt tttgcgtcac tcaaggcctc tcatgcaagt
//
LOCUS       DATASH010000015         6299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3958111,
            whole genome shotgun sequence.
ACCESSION   DATASH010000015 DATASH010000000
VERSION     DATASH010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6299
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3958111"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..141
                     /locus_tag="VHV99_00380"
     CDS             <1..141
                     /locus_tag="VHV99_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3633954.1"
                     /translation="RPLKRAIQQQIENPVAKLILAGKFGPKDVIPVDMRDGELVFDRV
                     VH"
     gene            complement(420..869)
                     /locus_tag="VHV99_00385"
     CDS             complement(420..869)
                     /locus_tag="VHV99_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008349311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HEX3633955.1"
                     /translation="MSLLDTLASSFGNSSQGGGAQSGLVAIAMEFVNNQPGGLNGLIQ
                     RFHENGAGDIVSSWISNGENKAISSDTVTNVLGSDAVSQMAQKAGLSGDQVSGILASV
                     LPHLVNHATPDGQVPADGKLDVGSLLGSLGGLGGLAGLFGKSEEPKA"
     gene            complement(1070..1687)
                     /locus_tag="VHV99_00390"
     CDS             complement(1070..1687)
                     /locus_tag="VHV99_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX3633956.1"
                     /translation="MLKKLLMLCLALALSIGAAFAAVDVNTADQTSLEAVKGLGPVKS
                     KAIIDERTKNGPFKDADDLANRVKGLGTKSVTNLEQAGLTIGGSSAPPTGTKASKTPA
                     ATNAGTAAPAKSTVKSSTAATAATSPAATTATTPATAPAATPDAASTTKGKKGKKAKT
                     SAAASSSAATSTAPAATADAASGASGAKASKKSKKKKAASDAAAT"
     gene            complement(2014..4233)
                     /gene="pbpC"
                     /locus_tag="VHV99_00395"
     CDS             complement(2014..4233)
                     /gene="pbpC"
                     /locus_tag="VHV99_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HEX3633957.1"
                     /translation="MKRAALALIGCVASVASVVYASPANALPSFDEVRASWRSSDWVL
                     LARNGEPLQRTRIDATERRGDWVALQDVSPALREAIVVSEDKRFYEHSGVDWQGAAAA
                     AWANLWNTRTRGASTVTMQLTGLIDEDRRPGRRSIGEKATQTVGALWLDHAWRKDQIL
                     EAYLNLVPFRGEIVGLSALSQTLFGKLPSGLNEREAALTAALVRAPNANYVKVSARAC
                     GILRDMSRARPSNMDPCANLDSYVQLTLTRTTSASPRSSDYDALAPHFARRVAGEVHP
                     AAGARVRSTLDASLQRFARDSLARTLLELNSRAHQRNVHDGAAIVIDNRSGEILAWVG
                     SAGGLSNAPEVDAVVALRQAGSTLKPFLYAQAIDEKRLTPASLLDDAPLDLATGGGLY
                     IPQNYDHDFKGWVSMRTALGSSLNVPAVRTLVMVTPHRFAKTLVSLGLPLTQAGDYYG
                     FSLALGSADVTLATLTNAYRTLANGGLTGPVSDLPTHAPPSPGTRVFSPQASFIVTNI
                     LADNNARTRTFGFDSVLATRFFSAVKTGTSKDMRDNWAVGYTSRYTVGVWVGNADGAP
                     MWDVSGVTGAAPVWAALVNYLHRRTPSQAPAPPTGVVQTKIVYQNDIEPARDDWFLTG
                     TQMSRISLSANAEGAAVRNGTARIASPTDGTIFALDPDIPAARQRIWFESAQSAPEGS
                     VWRLDGKLAGPSAQMKRMTWLPWPGHHTLELVDRQGVVVDSVKFEVRGAFAKIKTKG"
     gene            complement(4238..>6299)
                     /locus_tag="VHV99_00400"
     CDS             complement(4238..>6299)
                     /locus_tag="VHV99_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007736420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HEX3633958.1"
                     /translation="NPRVTLDANGEAAVDVPLNDALTSFRIVAIGAVGTGQFGTGSAS
                     IRSTQDLQLISGLPPLVRVGDQFRALFTLRNTTTRTMQVVVTPHVPSLDLPAQNVTLA
                     PESSQEIMWDVKTPDAIGMAATGALNWEVSAAEQGGAKAADAVKLTQRVVAAIPVTVQ
                     QATITQVDGTFALPVAPPETAVASADGQIRGGIAVTLQPKLADGLPGVRRWFERYPYS
                     CLEQQTSRALGLDDLTQWQTVIARMPSYLDSDGLANYFPPSDDSRPYGSPALSAYLLA
                     VSDEASKADPRFALPDDLRDKLAGALTNFVEGKIERKFWSPRRDLDFVKLSAIEALSR
                     YGLAAPRMLGSITIAPNQWPTSALLDYYSILSRVDGIAQRDDQRAQVEQILRARLTYS
                     GTRLTFSTAADDDLYWLMTGPESNAARTALLFADNPAWKDEMPRLVGGLLALQTKGAW
                     PTTTSNLWGKLAVQRFSKVFESTPVSGQTRIALGTTERTIAWNAPASAANPRNVALPW
                     TSGTLNLTQDGAGKPWATIESLAAVALKAPFAAGYRITKTITPIDPAVKGTLSRGDIV
                     RVHLDIDAQSDMTWVVVNDPIPAGATILGSGLGRDSEAATEGEKSPQGAWPAFVERGF
                     DGYRAYYDYLPKGKLQVEYTVRLNNAGTFGLPPTRVEALYAPSVFGAIPNATMVVAPL
                     AAAK"
BASE COUNT         1142 a         1953 c         1976 g         1228 t
ORIGIN      
        1 cggccgctga agcgcgcgat ccagcagcag atcgagaatc ctgtcgcgaa gctgattctg
       61 gcaggcaagt tcggaccgaa ggacgtgatc cccgtggata tgcgggatgg cgagctggtg
      121 ttcgatcgcg tcgtgcatta aaggacgctt gccggattta cgccagtctt gatcaccgac
      181 tgttgctgtt gaagaacgcc cgacgaaggt ttgtcgggcg tttttttatc gtaagcgatg
      241 atcgccaagc ctacaaatat tgaaagcggg atcacgggcc ggggaattaa aaggccggag
      301 aaatggcgtt agaaacggcc caagcgccaa ggctcatctc accaacaaaa aggggcaacg
      361 gatcactccg ttgccccttt ttgcttcttt tactgctgcc caaaaaccgg gcgaaacgcc
      421 tacgccttcg gttcttcgct cttgccgaac aagccagcca gaccgcccag gccaccaaga
      481 gagcccagca agctgcctac gtccagcttg ccgtccgccg gcacttggcc gtctggtgtc
      541 gcgtggttca cgagatgcgg caacactgaa gccaggatgc cggaaacctg gtcgccggac
      601 aggccggctt tctgcgccat ctggctgacc gcatccgagc cgagcacgtt ggttacggtg
      661 tccgaactga tggctttgtt ttcgccgttg ctgatccacg acgaaacgat gtcgcccgcg
      721 ccattttcat ggaatcgctg aatcaagccg ttcaggccgc ccggctggtt gttgacgaat
      781 tccatcgcaa tggcgactag accggactgc gcgccgccac cttgagacga attgccgaag
      841 gaggaggcga gggtatcgag taggctcatg gctgtctcac ttgaaaacga tgctggttaa
      901 atgccgtccg gcgtcatgcc ggtttgccag tgccggacgg aaaacgtgcc gttcgttgag
      961 aaacggccag attgtttctg ctgcaaggcg acttgggcgg catgaattcg agctgcgcct
     1021 caatggccat ttccacgctc gggattcatg ccgaagtgcg tcgccgtcct caggttgcag
     1081 ccgcgtcgct ggcggctttc tttttcttgg acttcttcga cgccttggcg ccgctcgcgc
     1141 ctgaggccgc gtcggccgtc gctgctggcg ccgtactggt cgccgcactc gagctggcgg
     1201 ccgcgctggt cttcgctttt ttacccttct tgcccttggt ggtggaagcc gcgtccggcg
     1261 ttgctgcggg tgcggttgcc ggcgttgtgg ccgtggtcgc ggcgggtgac gtcgcggcag
     1321 tcgcggccgt ggacgacttc accgtcgatt tcgcgggcgc tgcggtgcct gcgttagttg
     1381 ctgccggtgt cttgctggcc ttcgtcccgg tcggcggtgc agatgaaccg ccgattgtca
     1441 gcccggcctg ttcgaggttc gtcacggact tcgtgccgag gcctttcacc cggttggcga
     1501 gatcgtcggc atccttgaac ggcccattct tcgtccgttc gtcgatgatc gccttcgact
     1561 tcaccggccc gagtcccttt accgcttcaa gggaggtttg gtccgccgtg ttcacgtcga
     1621 cggccgcgaa cgcggcgcct atggacaacg cgagggccag acacagcatc aacagctttt
     1681 taagcatgac aagtgctcca ggttgaaaaa ataaaaagag gaatcgatgc aggcctaact
     1741 ggagcctggc cagaaagtcc aacaagcata ggacaaaatg cgggtcagga taatggcagc
     1801 gacccccgtc cggtgtgagc gggattattt tttacaacat caccggctat tattttgccg
     1861 gttgtaccca atccgcagtc cagtttcaat tccgtttagc gattatcacc agactcatgg
     1921 cggatgaggg cgggtcggtt cagatttaac atttccggat ataagcttgt cggtattgac
     1981 gcgctcgttg cgcgaatgcc gggtttgatc tgattaaccc tttgtcttga ttttggcaaa
     2041 cgcgccgcgt acttcgaatt tcacggaatc gaccaccacg ccctggcggt cgaccaattc
     2101 cagcgtgtga tgccccggcc acggcagcca cgtcattcgt ttcatttgcg cagaagggcc
     2161 ggccagtttg ccgtcaagcc gccacacgct gccctccggc gcgctttggg cgctctcgaa
     2221 ccagatccgc tggcgggcgg cgggaatatc aggatcgaga gcgaagatag taccgtcagt
     2281 gggagacgcg atccgggccg tgccgttgcg tacggccgcg ccttccgcat tcgccgacaa
     2341 cgagattcga gacatttgcg taccggtcag gaaccagtcg tcgcgtgccg gctcgatatc
     2401 gttttgataa acaatttttg tctgcaccac gcccgtgggc ggtgccggcg cctggcttgg
     2461 cgtgcgccgg tgcaggtaat tcaccagggc agcccacacg ggcgcagcgc ccgtgacacc
     2521 ggagacgtcc cacatcggcg cgccgtccgc gttgcccacc catacgccaa ctgtatagcg
     2581 cgacgtgtag ccgaccgccc agttatcgcg catgtccttg ctcgtgccgg ttttcacggc
     2641 gctgaaaaag cgcgtggcga ggacgctgtc gaaaccgaag gtgcgtgtgc gcgcgttgtt
     2701 atcggcgaga atgttcgtca cgataaagct cgcttgcgga ctgaaaacgc gcgtgccagg
     2761 agagggcggg gcatgcgtcg gcaagtcgga cactggcccg gtgagaccgc cgtttgcgag
     2821 cgtccggtac gcgttcgtga gggtcgcgag cgtcacgtcg gcgctgccga gcgcgagact
     2881 aaagccgtag taatcgccgg cttgcgtgag cggcaggccg agtgacacca gcgttttcgc
     2941 gaaccggtgc ggcgtgacca tcactagcgt gcgcacggcc ggcacattca gcgatgaccc
     3001 cagcgccgtg cgcatgctca cccaaccctt gaaatcgtga tcgtagttct gggggatata
     3061 caggccgcca ccggtggcga gatcgagcgg ggcgtcgtcg agtaaagacg cgggcgtgag
     3121 acgcttttcg tcgatagcct gagcgtacaa gaacggcttc agcgtcgagc cggcctggcg
     3181 cagcgcgacg accgcatcga cttccggtgc gttcgacaat cctcccgccg agccgaccca
     3241 cgcgaggatt tcgccgctgc ggttatcgat gacgatcgcc gcgccgtcgt gcacattgcg
     3301 ctgatgtgcg cgcgagttga gttcgagcaa ggtgcgcgcg agggagtcgc gagcgaagcg
     3361 ttgcagcgag gcgtcgagcg tggaccggac ccgcgcgccc gctgccggat gcacttcgcc
     3421 ggccacacgg cgcgcgaagt gcggggcgag cgcgtcgtaa tccgacgacc gcggcgaggc
     3481 ggacgtggtg cgagtgagcg tcaattgcac gtagctgtcg agattcgcgc aagggtccat
     3541 attcgatggc cgcgcccggc tcatgtcccg caaaatcccg cacgcccgcg ccgatacctt
     3601 cacataattt gcattcggcg cacgcaccag tgcggccgtg agcgcagctt cacgctcgtt
     3661 cagtccggac ggaagtttgc cgaacaaggt ctgcgataaa gccgacaaac ccactatctc
     3721 gccgcgaaac ggcacgagat tgagatacgc ctcgaggatc tggtccttgc gccaggcgtg
     3781 atcgagccat aacgcgccga ccgtctgcgt tgctttctcg cctattgaac ggcgtcccgg
     3841 ccgacgatct tcatcgatca aaccggtcag ttgcatggtc actgtcgatg ccccgcgtgt
     3901 gcgcgtgttc cagaggttgg cccaggccgc ggccgctgcg ccttgccaat ccacgccgct
     3961 gtgctcgtag aaccgcttgt cctccgacac gacaatcgcc tcgcgcaacg ccggcgatac
     4021 atcttgcaac gcgacccagt cgccgcgccg ctcggttgca tcgatacgcg tgcgctgcag
     4081 cggttcacca ttgcgggcga gcagcaccca gtcggaactg cgccaactcg cgcgcacttc
     4141 gtcgaagctc ggcagcgcgt tcgccgggct ggcgtacacg acggacgcga cggacgcgac
     4201 gcaaccgatc agggcgaggg cggcgcgttt catcgcgtta tttggccgcg gccaagggag
     4261 cgacgaccat cgtcgcgttg gggattgcac cgaatacgga gggcgcgtag agcgcttcca
     4321 cgcgcgtagg cggcagcccg aacgtgcccg cattgttcag ccggaccgtg tattccacct
     4381 gcaatttccc cttcggcaga tagtcgtaat acgcgcgata tccatcgaaa ccacgttcga
     4441 caaacgctgg ccaagcgcct tgcgggctct tttcgccttc ggtcgccgct tccgagtcgc
     4501 ggccgaggcc cgagccgagg atcgttgcac cggccggaat gggatcgttg acgacgaccc
     4561 atgtcatatc gctttgagca tcgatgtcga gatgcacacg cacgatgtca ccacgcgaaa
     4621 gcgtgccttt gacggcagga tcgatcggcg tgatggtttt cgtgatccga taccccgccg
     4681 caaacggcgc tttcaacgcg acggccgcga ggctctcgat ggtcgcccat ggtttgcccg
     4741 cgccgtcttg cgtcagattc agcgtgccgg atgtccaagg caacgccacg ttgcgcggat
     4801 tcgcagccga ggccggtgca ttccacgcga ttgtcctctc cgtcgtgccg agcgcaatgc
     4861 gcgtttgacc gctcaccggc gtgctctcga acaccttgga aaagcgctgc acggcgagct
     4921 tgccccacag gttcgacgtc gtggttggcc acgcgccctt cgtctgcagc gcgagcaaac
     4981 cgccgaccag gcgcggcatt tcatccttcc acgcggggtt atcggcgaag agcaatgccg
     5041 tgcgtgccgc gttcgattcc gggccggtca tgagccagta gagatcgtcg tcggcggcgg
     5101 tggagaaggt gagccgcgtg cccgaatacg tcaggcgcgc gcgcaggatt tgctcgactt
     5161 gagcgcgctg atcgtcgcgt tgtgcgatgc catcgacacg cgaaaggatc gaatagtaat
     5221 cgagcagtgc ggacgtcggc cattgattcg gcgcgatggt gatcgagccg agcatgcgcg
     5281 gtgcggccag gccgtaacgc gacaacgctt caatcgctga gagcttcacg aagtccaggt
     5341 ctcggcgcgg cgaccagaat ttccgctcga tcttgccttc cacgaagttg gtcagcgcgc
     5401 ccgcgagttt gtcgcgaagg tcatcgggca acgcgaagcg gggatcggct ttcgatgctt
     5461 catcgctgac ggcaagcaga taggccgaca acgccggact gccgtaaggc cgggaatcgt
     5521 ccgagggcgg gaagtaattg gcgagcccgt cgctatccag atacgaaggc atccgtgcga
     5581 tcaccgtctg ccactgcgtg aggtcatcga gacctagcgc gcgggacgtt tgctgctcga
     5641 ggcaactgta cggataacgt tcgaaccagc gccgcacacc cggcaggcca tcggcgagtt
     5701 tcggctgcag cgtcacggca atgccaccgc gaatctgtcc atcagccgat gccaccgcgg
     5761 tctcaggcgg cgctacgggc aatgcgaacg taccgtcgac ctgggtaatc gtcgcttgct
     5821 gcacggtcac cggaatcgca gccaccacac gttgcgtgag cttgacggca tcggcggctt
     5881 tcgcaccgcc ttgttccgcc gcgctcacct cccagttcaa cgcgcccgtc gcggccatgc
     5941 caatagcgtc cggcgtcttc acatcccaca tgatttcctg cgatgactcc ggcgccagcg
     6001 tcacgttttg cgcggggaga tcaagcgaag gaacatgagg cgtcacaacg acctgcatcg
     6061 tgcgtgtggt cgtattgcgc agcgtgaaca acgcgcggaa ctggtcgccc acgcgcacga
     6121 gcggtggcaa gcccgagatc aactgcaggt cctgcgtgct gcgtatcgac gcactgccgg
     6181 tgccaaactg acccgtgccc acggccccga tagcgacaat gcgaaagctc gtcaacgcgt
     6241 cgttgagcgg cacgtctact gctgcttcgc cgttcgcatc gagcgtcacg cgcgggttc
//
LOCUS       DATASH010000016         4619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5145032,
            whole genome shotgun sequence.
ACCESSION   DATASH010000016 DATASH010000000
VERSION     DATASH010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4619
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5145032"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="VHV99_00405"
     CDS             complement(<1..729)
                     /locus_tag="VHV99_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEX3633959.1"
                     /translation="MIKKLIRKLFGQDSAPADDTPPAEAEADVADEASARNASSAGKA
                     HRKPARPAAQAVRDPDVPVIIPHDVHGIDQSLISRNAIRVTEGLQEAGHRAFIVGGAV
                     RDLLLGIKPKDFDVATDATPEQVQKLFRRARIIGRRFQIVHVQFGQEIIETSTFRALV
                     DPPAADAAPPRRLKRDELDRRTHAVDASGRVLRDNVWGEQHEDATRRDFTINAMYYDP
                     ATQTVLDYHNGMADMRARLLRMIGD"
     gene            complement(726..1412)
                     /locus_tag="VHV99_00410"
     CDS             complement(726..1412)
                     /locus_tag="VHV99_00410"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX3633960.1"
                     /translation="MANLALFDLDHTLIPTDSDHEWGRFMVKQGMVDAENFARENDRF
                     FADYKAGKLDIHAYLIAMLTPLAKYSRAQLADLHAQYMHEVITPAIFPVALELVREHR
                     EAGDLCCVVTATNEFITRPIAQAFGVDALIACEAETVDGQPHSPYTGRPTGTPSYKEG
                     KIVRTEAWLASLGKSWTDFEHSYFYSDSHNDIPLLEKVTDPIATNPDDTLRAHAQAKG
                     WRILELFQPS"
     gene            complement(1419..2189)
                     /gene="hda"
                     /locus_tag="VHV99_00415"
     CDS             complement(1419..2189)
                     /gene="hda"
                     /locus_tag="VHV99_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HEX3633961.1"
                     /translation="MLRQLTLDLGTPPPSTFDNFFAGANAELVTRLRELDNALAAGPV
                     PDRTFYIWGEAGSGRTHLLQALVHDAPPGHARFAGTQSSLAAFTFDPRVALYAIDDCG
                     SLSAAQQIAVFNLFNEVRAHPTSALVAAGNAAPIGMTVREDLRTRLGWGLVFHLAPLP
                     DEGKAAVLKHAARERGIMLADDVPAYLLTHFRRDMPSLMGLLDALDRFSLEQKRAVTL
                     PLLRIMLNSPDAEERRTAPTSSTSPAASSASPPPLQVK"
     gene            complement(2264..3340)
                     /locus_tag="VHV99_00420"
     CDS             complement(2264..3340)
                     /locus_tag="VHV99_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX3633962.1"
                     /translation="MQQPPSILTPVQRRAFSWLAIALGVGILLWLLSPVLTPFLLGAI
                     LAYILQPGVAWMVRRRVPRALAALLMMLLFSLLITLLVLLVLAVIQKEGPQLRQQVPV
                     FFTHVNTWLHPKLAVLGLADSLDFASIRDLVMGQLEGSAQTVAAYAWAYIRTSGNVMM
                     TVVGNAVLVPLVLFYLLYDWNRMLTRVQSVVPRRWLDKTLQLARDMDQMLSQYLRGQL
                     LVMGVLAAYYAIALTVAGFEIALPVGIFTGLAVFIPYIGFVTGLALALLAALLQFGDW
                     YGFGAVALIYGVGQIVESFYLTPRLVGERIGLHPLAVIFALLAFGQLFGFFGVLLALP
                     VSAILSVAMRELRQSYLASTLYNN"
     gene            3450..4517
                     /gene="purM"
                     /locus_tag="VHV99_00425"
     CDS             3450..4517
                     /gene="purM"
                     /locus_tag="VHV99_00425"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010116949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEX3633963.1"
                     /translation="MNQPKSAPNSTDSAQGLSYRDAGVDIDAGDALVDAIKPFAKKTM
                     RDGVLGGIGGFGALFEVPKKYKEPVLVSGTDGVGTKLRLAFQLNKHDTVGQDLVAMSV
                     NDILVQGAEPLFFLDYFACGKLDVGTAATVVKGIAQGCELAGCALIGGETAEMPGMYP
                     DGEYDLAGFAVGAVEKRKIIDGSTIAPGDVVLGLASSGIHSNGFSLVRKIIERAQPDL
                     NADFDGRSLADALMAPTHFYVKPLLALMQQIAVKGMAHITGGGLVENIPRVLREGLTA
                     ELDHRGWPMPPLFAWLQKHGGVADAEMHRVFNCGIGMAVVVSAADADAAIGLLSAAGE
                     QVWKIGVIRESAAGEAQTVVV"
BASE COUNT          843 a         1339 c         1602 g          835 t
ORIGIN      
        1 gtcgccgatc atgcgcaaca gacgggcgcg catgtcggcc atgccgttgt gatagtccag
       61 cacggtttgc gtggccggat cgtagtacat ggcgttgatc gtgaagtcgc gccgcgtggc
      121 gtcttcgtgc tgctcgcccc agacgttgtc gcgcagcacg cggccgctgg cgtccaccgc
      181 gtgggtgcgg cggtcgagct cgtcgcgctt cagacggcgc ggcggcgccg cgtcggcggc
      241 gggcggatcg accagcgcgc ggaaggtcga ggtttcgatg atttcctggc cgaactgcac
      301 atgcacgatc tgaaaccggc ggccgatgat gcgcgcgcgg cgaaacagct tctgcacctg
      361 ctcgggcgtg gcgtcggtcg cgacgtcgaa gtctttcggc ttgatgccga gcaggagatc
      421 gcgcaccgcg ccgccgacga tgaacgcgcg atgccccgct tcctgcaggc cttcggtgac
      481 gcgaatcgcg ttccgcgaga tcagcgactg atcgatgcca tgcacgtcgt gcggaatgat
      541 gaccggcacg tcggggtccc gcacggcttg ggcggccggg cgtgccggtt tgcggtgcgc
      601 cttgccggcg ctggacgcgt tgcgtgccga tgcctcgtcg gctacgtctg cttcggcctc
      661 ggcgggcggg gtgtcgtcag cgggcgccga gtcctggccg aatagcttgc ggataagttt
      721 tttgatcacg agggttgaaa gagttcgagg atgcgccagc ctttggcctg cgcatgggcg
      781 cgcaatgtgt cgtcgggatt ggtcgcgatc ggatcggtga ccttttcgag cagcgggatg
      841 tcgttgtgcg agtcgctata gaaatagctg tgctcgaaat cggtccagct cttgccgagc
      901 gaagccagcc atgcttcggt acggacgatc ttgccttcct tgtagctcgg cgtgccggtg
      961 gggcggccgg tgtatggcga atgcggctgg ccgtcgacag tttccgcttc gcaggcgatc
     1021 agcgcatcga cgccgaacgc ctgggcgatc gggcgggtga tgaattcgtt ggtggcggtg
     1081 accacgcagc agaggtcgcc ggcttcgcga tgctctctga ccagttccag cgcgaccgga
     1141 aagatcgcgg gtgtgatgac ttcatgcatg tactgcgcgt gaaggtcggc aagctgcgcg
     1201 cgcgaatatt ttgcgagcgg cgtgagcatg gcgatcagat aagcgtgaat gtccagcttg
     1261 ccggccttgt agtcggcgaa aaagcggtcg ttttcacggg cgaaattttc agcgtcgacc
     1321 atgccttgtt tcaccatgaa gcggccccat tcgtggtcgc tgtcggtggg aatgagcgtg
     1381 tggtcgagat cgaagagtgc gaggttagcc atgggggcct attttacttg aagcggcggc
     1441 ggggacgcgg atgatgcggc gggcgacgtt gaggaggtgg gcgccgtgcg ccgttcctcg
     1501 gcgtcgggcg agttcagcat gatgcgtaga agcggcagcg tgaccgcgcg tttctgttcg
     1561 agcgagaagc ggtcgagcgc gtcgagcaat cccatcaggc tcggcatatc gcggcgaaaa
     1621 tgcgtgagca ggtaagcggg gacgtcgtcg gcgagcatga tgccgcgctc gcgcgccgcg
     1681 tgtttcagca ccgcggcctt gccttcgtcc ggcagcggcg cgaggtgaaa cacgaggccc
     1741 cagccgaggc gcgtgcgcag atcctcgcgc accgtcatgc cgatcggcgc cgcgttgccc
     1801 gcggcgacga gcgcgctggt gggatgcgcg cgcacttcgt tgaacaggtt gaacacggcg
     1861 atctgctgcg ccgcggagag gctgccgcaa tcgtcgatcg catacagcgc gacgcgcggg
     1921 tcgaacgtga aggcggcgag gctgctctgc gtaccggcaa agcgtgcatg acccggcggc
     1981 gcgtcgtgaa ccagcgcctg cagcagatgt gtgcggccgc tgcccgcctc gccccagatg
     2041 tagaaggtgc gatcgggcac tggcccggcc gcgagcgcgt tgtcgagttc gcgcagccgc
     2101 gtgaccagct cggcgttggc gccggcgaag aaattgtcga atgtcgatgg cggcggggtg
     2161 ccgagatcga gcgtcagttg acgaagcaca gtaggttaat gccgaaaatg aagcttgggc
     2221 cgcagatggc ccggacctcg ggccgtaaaa attcgatgag cggtcaattg ttgtaaagcg
     2281 tgctcgccag atagctttgg cgcaactcgc gcatcgccac cgagagaatc gcgctgaccg
     2341 gcagcgccag cagcacaccg aaaaagccga acagctgacc gaacgcgagc aacgcgaaga
     2401 tgaccgcgag ggggtgcagt ccgatccgtt cgccgacgag gcgcggcgtg agataaaagc
     2461 tctccacgat ctggccgacg ccgtaaatca gcgcgaccgc gccgaagccg taccagtcgc
     2521 cgaactgcag cagtgccgcg agcagcgcca gcgcgagacc ggtgacaaag ccaatgtacg
     2581 ggatgaacac agcgaggccc gtgaaaatac ccaccggcag cgcgatttca aacccggcga
     2641 cggtcagcgc aatcgcatag taggccgcca gcacccccat cacgagcaac tggccgcgca
     2701 ggtattgcga cagcatctgg tccatgtcgc gcgcgagctg cagcgtcttg tcgagccagc
     2761 ggcgcggcac cacgctctgc acgcgggtga gcatgcggtt ccagtcgtac agcagataaa
     2821 acagcacgag cggcaccagc acggcattgc cgaccaccgt catcattacg ttgccgctgg
     2881 tgcggatgta ggcccatgca tacgcagcga cggtctgtgc gctgccttcc agctgcccca
     2941 tcacgagatc gcggatgctg gcgaaatcga gcgaatcggc aagtcccagc acggcgagct
     3001 tggggtgcag ccacgtgttg acatgcgtga aaaacacggg cacctgctgc ctgagttgcg
     3061 gcccctcttt ctgaatcacg gccagcacca gcagcacgag cagcgttatc agcagcgaga
     3121 acagcagcat catcagcagg gcggcgagtg cgcgcggcac gcgccggcgc accatccacg
     3181 ctacgcccgg ttgcagaatg tacgcgagaa tggcgcccag caggaacggc gtcagcaccg
     3241 ggctcaggag ccacagcaga atgccgacgc ccagcgcgat tgccagccag ctgaaggcgc
     3301 ggcgctgcac gggtgtcaag atcgaggggg gttgttgcaa agttgtttcc ctggtgtcgt
     3361 cacgacagga tgatcaggac gattcaaatt ccagccagcc ggccggcatc gggtaaaatc
     3421 gcattttacc gaccttcgag ctcttcccca tgaatcaacc gaaatccgcc ccgaattcaa
     3481 ctgattcggc ccaagggttg tcgtatcgcg acgccggcgt ggacatcgac gcgggcgacg
     3541 ccctggtcga cgcgatcaag ccctttgcca aaaagacgat gcgcgacggc gtgcttggcg
     3601 gcatcggcgg gtttggcgcg ctgtttgaag tgccgaagaa atacaaggag ccggtgctgg
     3661 tgtcgggcac ggacggtgtc ggtaccaagc tgcgcctcgc gtttcaactg aacaagcatg
     3721 acacggtcgg ccaggatctg gtcgcgatga gcgtcaacga cattctggtg cagggcgccg
     3781 agccgctgtt tttcctcgat tacttcgctt gcggcaagct ggacgtcggt accgcggcaa
     3841 cggtcgtgaa ggggatcgcg cagggttgcg aactggcggg ttgcgcgctg atcggcggcg
     3901 agacggctga aatgccgggc atgtatccgg acggtgaata cgatctcgcc ggttttgcgg
     3961 tcggcgcggt ggaaaagcgc aagattatcg acggcagcac gatcgccccg ggtgacgtgg
     4021 tgctgggttt ggcttccagc ggcatccatt cgaatggttt ttcgctggtg cgcaagatta
     4081 tcgagcgcgc gcagcctgat ctgaatgcgg acttcgatgg ccgctcgctg gccgatgcct
     4141 tgatggcgcc tacgcatttt tatgtgaagc ccttgctggc cttgatgcag cagatcgctg
     4201 tgaagggcat ggcgcacatc acgggcggcg gcctcgtcga aaatattccg cgcgttttgc
     4261 gtgaaggtct gacggctgaa ctcgatcatc gcggctggcc gatgccgccg ctgttcgcgt
     4321 ggctgcagaa gcacggcggc gtggcggatg cggaaatgca ccgcgtgttc aattgcggaa
     4381 tcgggatggc ggtggttgtg tctgccgctg atgccgatgc ggcgatcggg ctgttgtcgg
     4441 ctgccggcga gcaggtctgg aagatcggcg tgattcgcga aagcgcggcc ggtgaagctc
     4501 agacggtcgt ggtttaaaca gtcagcttaa acagccggct tgaacaacgg ctggtttgcc
     4561 tgcgcggtgt acagaaagaa agcgccgcgt gggcgaacac ctcaaatcaa ttcttcaat
//
LOCUS       DATASH010000017         3005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1189011,
            whole genome shotgun sequence.
ACCESSION   DATASH010000017 DATASH010000000
VERSION     DATASH010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189011"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1319
                     /gene="paaN"
                     /locus_tag="VHV99_00430"
     CDS             <1..1319
                     /gene="paaN"
                     /locus_tag="VHV99_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaN"
                     /protein_id="HEX3633964.1"
                     /translation="IGYSVMHTTGQAFMMAFQAGGPHAQDRALEAVAYAWDQLRRIPA
                     DAHWEKPQGKNPPLAMHKRYTVVPRGAGLVLGCCTFPTWNGYPGLFADLATGNTVIVK
                     PHPGAILPLALTVRIARDVLREAGFDPNIVTLLATDPNDGPLVQDLALRPEIKLIDFT
                     GSTQNGTWLERNAHQAQVYTEKAGVNQIVIDSVDDIKAAARNIAFSLALYSGQMCTAP
                     QNIYVPRGGIRTAGGTLSFDEVAQAIAGAVQKLVADPARAVELLGAIQNEGITQRIDE
                     AAKLGRVLAESQTLEHPAFAGARVRTPLVLQLDAANDHAQFTKEWFGPISFVIATDST
                     AQSLDLAGAIAAEHGALTLSVYSTDEAVLEEAHEASIRGGVALSINLTGGVFVNQSAA
                     FSDFHGTGANPAANAALADAAFVANRFRVVQSRVHVEPNAAPAVAG"
     gene            1446..2648
                     /gene="pcaF"
                     /locus_tag="VHV99_00435"
     CDS             1446..2648
                     /gene="pcaF"
                     /locus_tag="VHV99_00435"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006415854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HEX3633965.1"
                     /translation="MNDAFICDAIRTPIGRYGGALKDVRADDLGAVPIKALIDRNPGV
                     DWRALDDVIYGSANQAGEDNRNVARMSALLAGLPTDAPGATINRLCCSGMDAVGTAAR
                     AIKAGEARLMIAGGVESMTRAPFVMGKATSAFSRQADIYDTTIGWRFINPLMKRQYGV
                     DSMPETAENVAVEFGIGRADQDAFALSSQQKAARAQRDGTLAQEIVGVEIAQKKGDLV
                     HVTLDEHPRETSLESLGKLKGVVRPDGSVTAGNASGVNDGACALLLANQDAADQYGLR
                     RRARVVGMATAGVEPRIMGIGPAPATQKLLKQLNMTLDQFDVIELNEAFASQGLAVLR
                     TLGLRDDDPRLNPNGGAIALGHPLGASGARLITTALYQLERTNGRFALCTMCIGVGQG
                     IALVIERL"
     gene            2707..>3005
                     /locus_tag="VHV99_00440"
     CDS             2707..>3005
                     /locus_tag="VHV99_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006029796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX3633966.1"
                     /translation="MSYESIRVDIDTQSRVATITLNRPDKLNSFTRAMHEELSAALGE
                     VEASGARALVLTGAGRGFCAGQDLADLDFTPGAMTDLGKLIDTHFNPLIRRLQALP"
BASE COUNT          601 a          971 c          912 g          521 t
ORIGIN      
        1 agatcgggta cagcgtgatg cacaccactg gccaggcatt catgatggct tttcaggcgg
       61 gcggcccgca tgcgcaggat cgcgcgctcg aagcggtcgc gtacgcctgg gaccagttgc
      121 gccgcatccc cgccgatgct cactgggaaa agccgcaagg caagaatccg ccgctcgcga
      181 tgcacaagcg ctacaccgtc gtgccgcgtg gagccggcct tgtgctcggc tgctgcactt
      241 tcccaacgtg gaacggctat cctggcctct ttgctgatct ggcaacaggc aacacggtca
      301 tcgtcaagcc gcatccgggt gcaattttgc cgctcgcgct gaccgtccga atcgcgcgtg
      361 acgtattgcg cgaggccggt ttcgatccga acatcgttac gctgctcgca accgacccga
      421 acgacggtcc gctcgtacag gatctggcac tgcgcccgga aatcaaattg atcgacttca
      481 cgggcagcac acaaaacggc acctggctcg agcgcaatgc gcatcaggct caggtctaca
      541 ctgaaaaagc cggcgtcaac cagatcgtga tcgactcggt agacgacatc aaggccgccg
      601 ctcgcaatat cgccttctca ctcgcgctgt actcgggcca gatgtgcacc gcgccgcaga
      661 acatctacgt accgcgcggc ggtatccgca ccgctggagg cacgctcagt ttcgacgaag
      721 tcgctcaagc tatcgcaggc gcagtgcaaa aactggttgc tgacccagcg cgcgccgtcg
      781 aactgctcgg cgcgatccag aacgaaggca taacccaacg catcgacgag gctgccaagc
      841 ttggccgcgt tctggcggaa agccaaacgc tggaacatcc ggcctttgcc ggcgcacgcg
      901 tgcgcacacc gctcgtgctc caactagatg ctgctaacga ccacgcccaa ttcaccaaag
      961 aatggttcgg cccgatctcg ttcgtgatcg caacggactc gacagcgcag tcgctcgacc
     1021 ttgctggcgc aatcgccgcc gagcacggcg cgctgacgct gtctgtttac agcacggatg
     1081 aagccgtgct cgaagaggcg cacgaagcat cgatccgcgg tggcgtagca ctctcgatca
     1141 acttgacggg cggcgttttc gtcaatcagt cggcggcctt ctctgacttc cacggcacgg
     1201 gagcaaaccc ggcagcaaat gccgcgctgg ccgacgcggc ctttgtcgcg aaccgcttcc
     1261 gcgtcgttca aagtcgcgtt catgttgaac cgaacgcggc gccagcggta gctggctgaa
     1321 tggcataaga caaaatcacg gctttcgtcc tatgccgttt ggccgaatgg accaagccgt
     1381 gcgccccaac taacatgaga catcggttcg gcatgtcccg ccggcccctc ccagcaggaa
     1441 cccacatgaa cgacgcattt atttgcgacg cgattcgcac cccaatcggc cgctacggcg
     1501 gcgccctgaa ggatgtccgc gctgacgacc tcggcgctgt accgatcaag gcgctgatcg
     1561 accgtaaccc tggcgtcgac tggcgggccc tcgacgacgt gatctatggc tccgccaatc
     1621 aggccggcga ggacaatcgc aacgtcgccc gcatgtcggc gttgctcgcc ggcttgccaa
     1681 cggatgcgcc gggcgcaacc atcaaccggc tgtgctgttc cggcatggac gcggtcggca
     1741 ctgctgctcg cgccatcaag gctggcgaag cgcggttgat gatcgccggc ggcgtcgaaa
     1801 gcatgacgcg cgcgccgttc gtgatgggca aggccaccag cgcgttttcg cggcaggccg
     1861 acatttacga cacgaccatc ggctggcgct tcatcaatcc gctaatgaaa cgtcaatatg
     1921 gcgtcgattc gatgccggag accgcggaaa atgtcgccgt cgagttcggc ataggccgcg
     1981 cggatcagga cgcgtttgca ctgtcgagcc agcaaaaagc agcgcgcgcg cagcgcgacg
     2041 gcacgttggc tcaggaaatc gtcggcgttg aaattgcgca gaagaaaggc gatctggtcc
     2101 acgtgacact cgacgagcat ccgcgcgaaa cgtcgctcga aagtctcggc aagctcaagg
     2161 gcgtggtccg ccccgacggc agcgtgaccg ccggcaatgc gtcaggcgtg aacgacggcg
     2221 cgtgcgcact gctgctcgcg aatcaggacg ctgccgatca atatggattg cgtcgccgcg
     2281 cacgtgtggt aggcatggca acggcgggcg tggaaccgcg catcatgggt atcggcccgg
     2341 cgccggccac gcaaaagctg ttgaagcaat tgaacatgac actcgaccaa ttcgacgtga
     2401 tcgagttgaa cgaagccttc gcgtcgcagg ggcttgccgt cctgcgcaca ctcggtctgc
     2461 gcgacgacga tccgcgcttg aatccgaatg gcggagcgat tgcgctcggc catccgctcg
     2521 gcgcatcggg cgcgcgtttg atcaccacgg cgctttatca gctggagcgg accaacggcc
     2581 gctttgcgct ttgcacgatg tgtatcggcg tgggtcaagg cattgcgctg gtaatcgagc
     2641 ggctctgaca tcaacaccgc ttcgcccacc gtcccgacgg tgaaagagac tttgaggaga
     2701 cacccaatgt catatgaatc gatccgcgtc gacattgata cacagagccg cgtagcgacc
     2761 atcacgctga accgcccgga caagctgaac agctttacgc gcgctatgca cgaagagttg
     2821 agcgccgcgc ttggcgaggt cgaagcatcc ggcgcccgcg cgctcgtgct aaccggtgcg
     2881 ggccgcggct tctgcgcggg acaggatctc gcggatctcg acttcacgcc gggcgcgatg
     2941 acagatctgg gcaaactgat cgacacgcat ttcaatccac tgattcgccg cctgcaagcg
     3001 ctgcc
//
LOCUS       DATASH010000018         4377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_595688,
            whole genome shotgun sequence.
ACCESSION   DATASH010000018 DATASH010000000
VERSION     DATASH010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595688"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..751)
                     /locus_tag="VHV99_00445"
     CDS             complement(152..751)
                     /locus_tag="VHV99_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEX3633967.1"
                     /translation="MNTHADSLSDSTGTPVPSRYTRTAITLHWLIALLMIVNVVLGLT
                     AESWPDDWVRPVVDTHKSIGITVLGLALLRILWRLSHKPPPLPREFPSWEKIAAHVAH
                     FLLYLLMIALPLSGWLHDSAWKDAATHPMHLFNLFPWPRIGYVMNLDPALKETLHDRF
                     GALHTWLGYALYALLAMHIGGALKHQWIDRKSVLKRMVP"
     gene            complement(922..1521)
                     /locus_tag="VHV99_00450"
     CDS             complement(922..1521)
                     /locus_tag="VHV99_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEX3633968.1"
                     /translation="MTATKPIALNRLLHDIRACTVCAPVLPHAPRPVVEASADSRILI
                     IGQAPGARVHASGIPWSDASGARLRNWLGVSDETFYDASKFAIVPMGFCFPGKGASGD
                     LPPRPECAAHWHQALLAQLREVRLTLLIGQYAQCFGLGDAVGPTLTDTLLRWREYGPS
                     VVPLPHPSPRNIAWFKRNPWFEAEVLPTLQTRVTAALKA"
     gene            1680..1802
                     /locus_tag="VHV99_00455"
     CDS             1680..1802
                     /locus_tag="VHV99_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3633969.1"
                     /translation="MTDREKQKRHRRLILALAGKFYVYPANDAWSTAAPDAACM"
     gene            complement(1822..2925)
                     /locus_tag="VHV99_00460"
     CDS             complement(1822..2925)
                     /locus_tag="VHV99_00460"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEX3633970.1"
                     /translation="MSLSTTDHTMPPSSPTEPSDGWLIQSCERGWLPDSLIRIGMRQL
                     MRQRLRAEAIDNGELRAQRLNHLLDDLRASPIAIETKAANTQHYEVPASFFHAHLGAH
                     LKYSCCLYPTGSETLAQAEATMLERYAERAELADGQRILDLGCGWGSLSLWLAAHYPR
                     AQIVALSNSHGQRAFIEARAAEFGLRNLSVVTGNVVDFEFDDAQLGDGFDRVVSIEMF
                     EHMKNYGLLLAKISRWMRDEAKLFVHMFVHRTLAYHFQVQDSSDWMSKYFFTGGTMPS
                     EALLLNFQDDLRMERQWWVSGTHYERTANHWLAALDAARDRVMPMLVDTYGARDAAVW
                     FQRWRMFYMAVAELFGYANGNEWGVGHYRFVKR"
     gene            complement(2995..3771)
                     /locus_tag="VHV99_00465"
     CDS             complement(2995..3771)
                     /locus_tag="VHV99_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HEX3633971.1"
                     /translation="MPPLAAAVIASIGLVVIFSAVWAWQLKSENAGMVDPVWAYSLGL
                     VAVLYAVLGTGDPASRALTALGGLIWGVRLGKHLWKRNDGKPEDARYRRFREEWGDNA
                     ASKMFWFFQLQVVISLLLSLAFLVPSYRSRPAPIGWIVLAIALWIVSVAGESSADRQL
                     KRFTQDPANRDSVCRVGLWRYSRHPNYFFECVHWLAYIALSIGTPWGWFTLLPPVLMA
                     FLLLKLSGIPLLEESMAKRRAGYAEYMRTTSALIPWPPRQ"
     gene            complement(3857..4168)
                     /locus_tag="VHV99_00470"
                     /pseudo
     CDS             complement(3857..4168)
                     /locus_tag="VHV99_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906892.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
BASE COUNT          887 a         1374 c         1333 g          783 t
ORIGIN      
        1 gctgaaatga atcagcgtgc tcagcagcgt agtgggacgc ggcagaatgc gcggcgatgc
       61 gagggcgagc gcctgttcca aagtgttctt caaagtgact gctgagtccg tcgacggtgc
      121 ggttattaaa tgggaaacag catggcggtg ctcacggcac catgcgtttc agaaccgact
      181 tgcgatcgat ccattgatgc ttcaacgcgc cgccaatatg cattgccagc aacgcataca
      241 gtgcgtagcc gagccacgta tgcaaggcgc cgaagcggtc atgcagcgtc tccttgagcg
      301 ccgggtcgag attcatcaca tagccaatgc gcggccaggg gaacaggttg aacagatgca
      361 tgggatgcgt ggccgcatct ttccaggcgg aatcgtgtaa ccagcccgag agcggcagtg
      421 caatcatcag cagatacagc aggaaatggg cgacgtgcgc ggcgatcttc tcccacgacg
      481 gaaactcgcg cggcagcggc ggcggcttgt gcgacaggcg ccacaagatg cgcagcagcg
      541 cgaggccgag caccgtgatg ccgatcgact tgtgggtatc gaccaccgga cgcacccagt
      601 cgtccggcca tgattcggcc gtcaacccga gcacgacgtt gacgatcatc agcagcgcga
      661 tcagccagtg cagcgtgatt gccgtgcggg tatagcgcga cggcaccggc gtgccggtcg
      721 aatcggacag cgaatcggcg tgcgtgttca tggggcgctc cgtctgcaac tgcattcctg
      781 cgaaagctgt ttgaaaagtc ggccgtgccg gtgcaatgcc tgctctcgcc tccgatacaa
      841 aagctaacgc cttagtgtgc cggtttattc cgcgaggcgc ttaatagtgt gattcagtct
      901 atttcgaccg cgcgtggcga atcatgcttt gagcgccgcc gtcacacgcg tctgcaacgt
      961 cggcaacacc tccgcttcga accacggatt gcgcttgaac cacgcgatat tgcgtggcga
     1021 tggatgcgga agcggcacca cgcttgggcc atattcccgc caacgcagca gcgtgtcggt
     1081 gagcgtcggc ccgactgcgt caccgagacc aaaacactgc gcgtattggc cgatcagcaa
     1141 cgtgagccgc acctcgcgca actgggcgag caaggcctga tgccaatgtg cggcgcactc
     1201 gggacgcggc ggcaggtcgc cgctcgcgcc tttgccggga aagcagaagc ccatcggcac
     1261 gattgcaaat ttcgaggcgt cgtagaaggt ctcgtcggag acccccagcc aattgcgcaa
     1321 acgcgcgccg ctcgcatcgc tccatggaat gcccgaagca tgcacgcgcg cacccggcgc
     1381 ctggccgatg atcagaatgc gcgagtcagc tgacgcctcc acgaccggcc gcggcgcatg
     1441 cggcagcacc ggcgcgcaca ccgtacaggc gcgaatgtca tgcaatagcc gattgagggc
     1501 aatgggtttc gttgcggtca taccgtggcg cggatcggga ttcaggtttc tggtggataa
     1561 agcaacgtgc cgcccggctg cgcctgcgcg cccggtgtca tggtcgcggc aggggtcggt
     1621 ttctatccta tcctggatgc ttcgagcaaa tcggtgaact cgcgcagcgg acgcgttgaa
     1681 tgaccgatcg tgaaaaacag aagcgtcatc gcaggctcat cctggcgttg gcggggaagt
     1741 tctacgttta ccccgcgaac gacgcctggt caaccgcggc acccgacgcc gcctgcatgt
     1801 gaagcgacgc cctttagcgc gttagcgctt gacaaaccga tagtgcccca cgccccactc
     1861 gtttccattc gcatagccga acaactcggc cacggccata taaaacatgc gccaccgctg
     1921 aaaccacaca gccgcatcgc gagcgccgta ggtatcgacg agcatcggca tcacgcgatc
     1981 acgcgcggca tcgagcgcgg cgagccaatg attcgccgta cgctcgtagt gcgtgccgct
     2041 cacccaccat tgacgctcca tccgcagatc gtcctggaag ttcagcaaga gcgcttcgga
     2101 cggcatcgtg ccgcccgtga aaaagtactt cgacatccag tcgctgctgt cctgcacctg
     2161 aaagtgatac gcaagcgtgc gatgcacgaa catatgcacg aacagcttcg cctcgtcgcg
     2221 catccagcgc gagatctttg caagcagcag gccgtagttc ttcatatgct cgaacatctc
     2281 gatcgacacc acgcgatcga aaccatcgcc gagctgcgcg tcgtcgaatt cgaaatcgac
     2341 gacattgccc gtcaccaccg agaggttgcg caaaccgaac tccgccgcgc gtgcttcgat
     2401 gaacgcgcgc tgcccatgcg aattcgacag cgcaacgatc tgtgcacgcg gataatgtgc
     2461 cgccagccac aacgataacg aaccccatcc gcaaccgaga tcgagaatcc gctgaccgtc
     2521 ggccagttcc gcgcgttcgg catagcgctc gagcatcgtc gcttcggctt gcgcgagcgt
     2581 ttcgctgccg gtcgggtaca aacagcacga gtacttcaga tgcgctccga gatgcgcatg
     2641 aaagaacgag gccgggactt catagtgctg cgtgttggcg gcctttgttt cgatggcgat
     2701 cggactcgcg cgcaaatcgt ccagcaggtg gttgagtcgt tgtgcgcgca actcgccgtt
     2761 gtcgatcgct tcggcgcgca aacgctggcg catcagttga cgcatgccga tacggatcag
     2821 gctgtcgggc agccagccgc gttcgcagct ttggatcagc cagccatcgc tgggctcagt
     2881 tggcgaggaa ggcggcattg tatggtcggt ggtggaaagg ctcatgaggc tgactcctat
     2941 cgttttttct ctctggacac tactgaacgt tcgcacgcgc ctcccggcgc tatctcattg
     3001 ccgcggcggc catggaatca gcgcgctggt ggtgcgcatg tattcggcgt aaccggcgcg
     3061 ccgtttcgcc atgctttcct cgagcagcgg aatgcccgag agcttcagca gcagaaacgc
     3121 cattagcacc ggcggcagta acgtgaacca tccccacggt gtgccgatcg acaacgcgat
     3181 gtaagcgagc caatgcacgc attcgaagaa gtagttcgga tgccgcgaat agcgccacaa
     3241 gccgacacgg cacacgctgt cgcgattggc cgggtcctgc gtgaagcgtt tgagttgccg
     3301 gtccgcgcta ctttcgccgg ctaccgagac gatccacagc gcgattgcca gcacgatcca
     3361 gccgatcggc gctggcctgc tccggtacga cggcacgaga aatgcgagcg acagcagcag
     3421 cgaaatcacg acttgcagct ggaaaaacca gaacatcttg ctggctgcgt tgtcgcccca
     3481 ttcttcccgg aagcggcgat agcgcgcgtc ttcgggcttg ccgtcattgc gtttccacag
     3541 atgcttgccg agccgcacgc cccaaatcag gccgccgagc gcagtcaagg cacgcgacgc
     3601 tggatcgccg gtgccgagca cggcgtacag caccgcgacg aggccaagcg aataggccca
     3661 aaccggatcg accatgccgg cgttttcgct cttcaattgc catgcccaga cggcgctgaa
     3721 gatcacgacg agcccgatcg aggcgatcac ggcggcggca agtggcggca tggtggcctc
     3781 gttcaatggc aatgcggttt atacgctgta agggcacgac cggatgctgc gggcgcgcca
     3841 tccggcacat gtggctttag tccttgtcag ccggcgaggc gaaacctgct ttgccgagct
     3901 ggtcgggatg ctgcggcacg cgtctgaaac gcgacgtgtc gtagcccatc gcatcgagcc
     3961 gcacgagcag cgacttataa gtgtcgtcgc tcatgtcggg ctcacgcgaa aagatccaac
     4021 cgaggttttt gcctgggtag cccagaatgg tgtagcgata atcggggtcg acgtagagcg
     4081 tcagctgcgt cacgtggaca ggccagaaca gctgcacgct ccactcccca ccttccttgc
     4141 ccggcaccgg cgtgtcgagg aactgatatg acggtcacgg cgaggacgcc acccagcttg
     4201 ggcaggccgt cgccgcgctc aaggaaacga atcggaaacc caagccggtt cacagcgcga
     4261 aactctgcca gcgcctgagc gcgcaccgta ccgctgccat ccacgcggaa ctgattgcgc
     4321 agccatccgt cgccctcgct gccctgctgt tccggctcac gccggtcgcc ttccccg
//
LOCUS       DATASH010000019        18298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6925571,
            whole genome shotgun sequence.
ACCESSION   DATASH010000019 DATASH010000000
VERSION     DATASH010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 18298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18298
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6925571"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..1729
                     /locus_tag="VHV99_00475"
     CDS             155..1729
                     /locus_tag="VHV99_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEX3633972.1"
                     /translation="MSTTTAERATTATTATSTASAGYRTLWRWHFYAGLFVMPFLVVL
                     AITGTVYCFQPQIEPLLYPHRLIVEPQASQRLTEDALLAKARAAMPANARAVTAPIAN
                     APDRSTEFIFRLANGEKHSVYLNPYSGEVLGTLSVERRFMQVDRMLHRKLLLGKPGEL
                     LMELAACWTLVMIGTGVALWWPRGTTTARAALVPRFSLRGRALWKNLHAVMGIWLALG
                     ALAFVLSGLPWSGSWGKQFKALATAANLGAPPGSWGGLPLRSALPGMRTGEHADTVAT
                     SAETQSANGVGVGVGVGASHDERPAHAAGDEHAEHSAHAANMDSMPGMVMDDLPLPLT
                     PWAIGNTPLPASADTHAAQPLPLGRIVSLVASLGVPNGYDIVLPASATGVYTVSYFPA
                     DPKDERTLYIDQYSGAVLKDIRYGDYGAVSKAVSYGTSLHMGRYFGLANQILCAAISL
                     GLAGMAVTGCVMWWKRRPQRSLGAPSRERAAPPMRGWKAGLVLLGIVFPLMGATLLAV
                     WLADRTIFGRATRQAA"
     gene            1766..2215
                     /locus_tag="VHV99_00480"
     CDS             1766..2215
                     /locus_tag="VHV99_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013689810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEX3633973.1"
                     /translation="MTTPRKLLLAALLCGSSMQAFAGAAATASVSDCWIRALPGDLPS
                     GGYFRASNRGDQPVNLIGVDSDAFGMAMLHQTQSQGSTSSMAMVEQVAVPAHGSLVFG
                     PGNYHVMLEHPKQPLKIGTTIPLTFSFSDGEKVTAACTVKSAGTMAP"
     gene            2429..3199
                     /locus_tag="VHV99_00485"
     CDS             2429..3199
                     /locus_tag="VHV99_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEX3633974.1"
                     /translation="MDGLLPEERERLILERLRAQGRVLASELAAEFQTSEHTVRRHLR
                     ELAEQGHCKRVYGGALLISPSGESAAIRLREAVDRKACLAVAAASIVRPKQIVLLDTG
                     STNAAIAAALPDNADLTVVTNSPEACARLLNRPGFDIILIGGRIVRHAAGSTGATALL
                     QVQQIKADLCFLGACAFDPDEGVAAFDAEEAELKRAMVKASSQVAIAMTSEKLMTAAP
                     FSVAPASAVDYLFVEADVTAERRALLQTVCDNVMVTRS"
     gene            3196..4374
                     /locus_tag="VHV99_00490"
     CDS             3196..4374
                     /locus_tag="VHV99_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3633975.1"
                     /translation="MKSLAKPFVQPVTKPLKERVATLGVFLANGFGIGAWAVEVPRIK
                     ESLALSDASLGIALFAFALGAIVAMPLAGQLAPRFGSGRATALLGAAFVVALPLPAFA
                     PNLATLCIVLLALGAANGALDVSMNGHASTIETQWKAPIMSSFHAAWSAGGLLGAATG
                     AMIEKGGVGVIGGLVLPGVVIAILIAAAAVLALRDLGPRAAAASSGFALPNAAVMKLA
                     LLAFLCMLVEGAVADWSAVYLRATLSEEASVAAVGYSAFALSMAACRIVGDVSVRRFG
                     SSKVIGFGGLLAAAGLALVLSLPSVFTACVGFAMVGVGLANIVPVIFSAAGRSTVTPA
                     VGVSMAATAGYAGFLVGPPLIGFGAGLFGLRLALCVLVVATLIVCLTGGKAVRNASLA
                     "
     gene            complement(4533..5096)
                     /locus_tag="VHV99_00495"
     CDS             complement(4533..5096)
                     /locus_tag="VHV99_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX3633976.1"
                     /translation="MLSGFTFAAVMQWRAAPTRITLLLLVVSAFVTVGLSLITRVPVR
                     RDWRPLAFICAMGGTYYYLAVRLNPGTHLIPETVGACLQVLGIFWQLFAKASLRRSFG
                     ILPANRGVVSRGAYRFVRHPMYLGYFITDIGFLLTNFGLQNVIVYACQFALQVVRIVR
                     EEQLLSDDERYRTYKGKVRYRVIPGVF"
     gene            complement(5248..6594)
                     /locus_tag="VHV99_00500"
     CDS             complement(5248..6594)
                     /locus_tag="VHV99_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX3633977.1"
                     /translation="MKTAAYKHQKGSISIIVAVSLVALLGILGLAIDSGLGYMIRARL
                     DAATDGAVVAAGQAVTRGNNQSEQTTNATQAANAFFTANYPAGFLGSTATAGTPSVVF
                     DNKGTVTINMSAQAQVPVTFMQTLGFKVLNVGASSQAIRKTLDMAFVIDTTGSMATDP
                     TEPPVVQANAVSFLNSFDITNDRVALMHFAYGTIIDTPFKKDSSRGFDRATMTAQIGK
                     YNFSGSTNSSEAFWNARNQMNQLQNPSSLRVIVFFSDGAPNSFASAFTNKVKACNNLV
                     GTIASPDSNGKMTGLWRYDQLQTAFSSPCYDSDLRADSSKFIPSLPAFYNAHNPADTT
                     FPIITNSPRVVTAAVTYQNVNRASRNLLEAMAADARNNNIYVFTLGLGQPLVQPAGPD
                     NELGQDVLKCMANTPDSLPRCYNPKQPVGVYCHAATPTDLAPCFSQLASQILRISQ"
     gene            complement(6624..7265)
                     /locus_tag="VHV99_00505"
     CDS             complement(6624..7265)
                     /locus_tag="VHV99_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEX3633978.1"
                     /translation="MKTKPWGKSRRKAAGIVSVELALLLPVLVALALPVVDIARNIQA
                     QMILINVSREGANLSSRASLTYPMQTIMTSLTATTPPLDMNANGMIYITEIMGNNNCD
                     ANGNNCTGVVVAQYRWNGGNYAPASKLWTCGGSGGTSTSWATDGTGSCSGLPAPGTKS
                     PVVNLLQGKLSSGQIAFAVEAFYLQTPLIGSLKLGGGITTPALSPNLYAMTVF"
     gene            complement(7267..7779)
                     /locus_tag="VHV99_00510"
     CDS             complement(7267..7779)
                     /locus_tag="VHV99_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007731090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEX3633979.1"
                     /translation="MKAARPQLKQAPKRTLKQRGVVTVEFALLAPLLFLLLCIVMDMG
                     IALWVNLTMQYAVREGARYAVTGQSNLDPNATNQQRYLAIVQEIKNSSMGLYALVSPS
                     YVITVNGGAAQTYNTQASYGTGMFGNPGDIVVLQINCIWPLLTPLVSPFFAGGKYTFS
                     VATTMRNEGF"
     gene            complement(7824..8672)
                     /locus_tag="VHV99_00515"
     CDS             complement(7824..8672)
                     /locus_tag="VHV99_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018423379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX3633980.1"
                     /translation="MFESNPLPVLWHFAHEWWLTYEIVLALFIVGALLERRWPVERLQ
                     SRTATRLNLAYSAISTAFGRATQPLTAAASVWIVNLLGGGLFALPARGWGVLVSILAV
                     VLTIDLLEYWFHRLQHAWPLLWRMHSFHHSAESFNVTVTTRHYWFEALLKGCVMYPLV
                     GVLFKVEPSIVGVATLIFLPGNFFAHLNLRIDLGRFITWVNNPQYHRLHHSRRTEHFD
                     KNFTQLLPLWDRLFGTMWVPAPHEWPQTGLDDDAEPASVLVALYWPLRKRTAGEPVAA
                     TQRAAE"
     gene            complement(8717..9664)
                     /locus_tag="VHV99_00520"
     CDS             complement(8717..9664)
                     /locus_tag="VHV99_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX3633981.1"
                     /translation="MRTEQIVVLALMFIIVFGAMLKAMMLLRPDPLQRRIDRVGAPTS
                     PDESDSGEQKTWVEAVTKMSHHVAKLSLPKEDWDKSTLRLQFAKAGIRNPSAPALYFA
                     AKTLLALVLPAIALIFCGNLFTPERRMWLLLAVLAASALGFYLPNLILSRMIEHRQRK
                     LFEDLPDALDLMTVCVEAGLGLDAAMMRVTQEIGVRSHALKDEFELVLLELRAGSGRD
                     KALRNLSLRTGVEDIDTLAAMLIQADRFGTSVGDSLRVYTDNLRTKRRMRAEEKAAKI
                     ALKLLFPLMFFIFPALITVLIGPSAIQVMRQLVPAMSGI"
     gene            complement(9744..10721)
                     /locus_tag="VHV99_00525"
     CDS             complement(9744..10721)
                     /locus_tag="VHV99_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007731088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX3633982.1"
                     /translation="MNTSFIGFAVLAFLAIVLLIESIYLFWSSHHSPVVKRMDERIRA
                     LSAGGNVSAEQLSILKQRLLSESPLVTRLLLVLPRVHTLDRQLQQSGLTWSVARFVTY
                     TLLSALIGMFIGFVLHAPWTVMLGLAGASALLPLLILRHKRGKRVLQLERQLPDAADL
                     ISRALRAGHSFPAALGMVGEELPNPLGGEFALVFDEINYGVSMNDALLNMVNRVPVDD
                     LRYFVIAVLIQREAGGNLAEILGSISSIIRERLKLLGKVRVLSAEGRLSAWILAVLPF
                     AVMGVLSLLNPGYIKVFWRDPLGIRLGMSALTMMLFGILWMRKVIRIRV"
     gene            complement(10768..12144)
                     /locus_tag="VHV99_00530"
     CDS             complement(10768..12144)
                     /locus_tag="VHV99_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014617784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HEX3633983.1"
                     /translation="MSLREQMSIQNGFMPAETAGAAAAGVAAESLAARRAYQQLKMTI
                     HQTIIERVELDKLQRLSPEQVKRELAQLVERIVEEDKIPMNELERRRLAQDVHDEMVG
                     LGPLEPLLNDPTISDILVNTSQHVYVERRGRLEHTDVTFYDDAHLMKIIERIASRVGR
                     RIDESTPMVDARLPDGSRVNAIIPPSAIDGPLVSIRRFAVNPLTVTDMVNNQSFTPAM
                     AQLLEALIKSKLNVLISGGTGSGKTTLLNLLSGFIPDDERVVTIEDAAELQLRQQHVL
                     RLETRPPNIEGKGEISQRSLVRNALRMRPDRIVLGEVRGAEALDMLHAMNTGHEGSMA
                     TLHANTPRDGLTRLENMVGMAGLTMPIKAIRQQIASAITVVVQASRLTDGRRKLMSIQ
                     EITGMEGDIINMQEIFTFKRIGVDENGMIKGYFCATGVRPKFCDRLAGFGITLPDQMF
                     DPARRLDV"
     gene            complement(12170..13372)
                     /locus_tag="VHV99_00535"
     CDS             complement(12170..13372)
                     /locus_tag="VHV99_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007583596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3633984.1"
                     /translation="MIDILMISSSAERSTHIGARLEASGVAYRLRVVHGEARQLRAHA
                     AAIKTADLLIVDDVDLSELELSGIEEALVQTPHLHCMLITPAPSTNLLMAAMRVGVRH
                     VLSWPLDEREIVDALTHVANKKNAGMRREGRVVSVSSCKGGSGATLIAVNLAYTLAAQ
                     RDKRVLLIDLNQQFADASLLVADKAPRATLTDLCSQIDRLDAAFFEACVMHIHANLDV
                     LAGAGDPVKAAELRPAHLERMLMLVREQYDAVIVDVGQSINPLAIHVLDHSDSICMVI
                     RQNILYLHSGRRMLDIFKELGYSASKVRLIVNQYDKNARISLATLEETLGAKVAHQLP
                     RDEKHANDALNHGIPVVTAAKGSALALGIGALAGMLWPIAAAPRKGVLSRFFAARPNS
                     LQTLKTEH"
     gene            complement(13418..13711)
                     /locus_tag="VHV99_00540"
     CDS             complement(13418..13711)
                     /locus_tag="VHV99_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3633985.1"
                     /translation="MKAIPTLIRLALCAGVTATLAGCLTSTPHWDATFGDSVNQLKAM
                     QTLNPEASANTDPVLGIDGTAAAAAQKGYAKSFTAPTPPTNMFTIGVGSGSSY"
     gene            complement(13748..15640)
                     /locus_tag="VHV99_00545"
     CDS             complement(13748..15640)
                     /locus_tag="VHV99_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012404563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein family
                     protein"
                     /protein_id="HEX3633986.1"
                     /translation="MNARPNHRAHAASAPHVTQLRTRLRIAQASACGLIGTCLWALPA
                     TQAAAQNVPALPDTPLTVAANGPMHLTIGMSGSGAPSNGSSQAGKGPSCTGPVADHTS
                     VQIPVGKSTMVDLREPVRTRTVGNPSIVQAMLVSPTTLYLLGSDVGTTNMIVQGKSGS
                     CTIIDVNVGADPAGLQQTLAVLMPEETGVRVKAAADTLVLTGVVSDSVKAERIVELAH
                     AFVARPTVAVPSAAPAAGPLPIGGVRVAMPPPPTLGSGNSERVINMMHIAAPQQVMLE
                     VKVAEVSKTLIDQLGMAANLNGGIGSWSFGLLANYLSGGLSAITASKANNSPLNLAFD
                     AQKTDQLVKILAEPNLMAISGQEASFLAGGKVYIPVPQSNGANGTTIVLQEEQYGVGL
                     TFTPTVLEGGRINLKVAPEVSELSSTGVVVSAGNSNNTTILPLITTRRASTTLQVYDG
                     QSFAIGGLIKNNVTGTLKGVPGVGELPVLGALFRSTNYEQDKTELVFVVTPRLAKPLP
                     QNYPLPTDGFVDAAEGNVYLTGNMEGKKYAAPAAGASSGATAASPVLAAPTLPPQPAP
                     APDRNPVPVSGPARVPAPDDNHGTPESQPLTSTKPNDAPHSEPVPAAAVVSAALPADA
                     DSHAAR"
     gene            complement(15691..16533)
                     /gene="cpaB"
                     /locus_tag="VHV99_00550"
     CDS             complement(15691..16533)
                     /gene="cpaB"
                     /locus_tag="VHV99_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003265863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEX3633987.1"
                     /translation="MKNARALIMLLIATMAGLTAVVFAARWMTSQTSSATTKVVVAVS
                     DINLGQRISPDFLKVADWPSNSVPPGSFSDMQKLDGRVLKESLLHGEPILEAKLSPVG
                     TLGGLSAVINEGKRAITVRVNDVIGVAGFALPGNYVDIIVNTQKDSKDPGTQRDQNIS
                     KIVLEKILVLAVAQEVGRDETKPKVVDAVTLEVSPEQAEKIDLARSIGTLSLVLRNQV
                     DPQATDTSGATKASLLKIPVAVPAAPASAPAPAVHIVRKVAARAPGNCVRVLDGADSR
                     QECF"
     gene            complement(16644..17177)
                     /locus_tag="VHV99_00555"
     CDS             complement(16644..17177)
                     /locus_tag="VHV99_00555"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEX3633988.1"
                     /translation="MNGVPFPIGPCVLALVMIAALSDLHTRRIPNWLIATALVVALPV
                     QVVLHGAAEGMQMWLGGCVTGGLLLLPGYLMRLMGAGDVKLMAAVGAFCGAYGAFEIG
                     LATFAMGGAWSLAVLLRRRQMLTGLIGARSLLAERAVPGDATAQRRSGLAHGSIGTIP
                     YGVAIAIGSTVVLFASV"
     gene            complement(17297..17479)
                     /locus_tag="VHV99_00560"
     CDS             complement(17297..17479)
                     /locus_tag="VHV99_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEX3633989.1"
                     /translation="MKNTIQQFLREEDGVAAIEYGLLAGLIAVGIIVASAALGADISN
                     LFNNIGNKLTGIAIPA"
BASE COUNT         3243 a         5819 c         5725 g         3511 t
ORIGIN      
        1 ttgtcctgtg cttgtcctgt gcttgccgat gtgcctgcgt ggtgttcgcc tggcaactcg
       61 ccagtgcctc gtcctcgatg cactagcgag ttgcaaaccc acctatgcat cgctgcgcaa
      121 accgatcgac ctactcattg cttcaggagg atgaatgtcc accaccaccg ctgaacgcgc
      181 gaccacggcg accacggcga ccagtacggc gagcgccggc taccggacgc tctggcgctg
      241 gcacttttat gcaggtctct ttgtgatgcc gtttctggtc gtgcttgcga tcaccggcac
      301 tgtgtattgc ttccagccgc aaattgagcc gctgctttat ccgcatcgct tgatcgtcga
      361 accgcaagcc tcacagaggc tgaccgaaga tgcattactc gcgaaagcgc gcgcggcgat
      421 gcccgccaat gcacgcgcag tgaccgcgcc catcgcgaac gcccccgatc gcagcaccga
      481 gttcatcttt cgcctcgcca acggcgaaaa gcacagcgtc tatctgaacc cgtacagcgg
      541 tgaagtattg ggcacgctga gcgtcgagcg ccgcttcatg caggtggacc gcatgcttca
      601 ccgcaagctg ctgctcggca aacccggcga actgctgatg gagctggccg cgtgctggac
      661 cctggtgatg atcggcaccg gcgtcgcact ctggtggccg cgcgggacaa ccacggcgcg
      721 cgcggcgctc gtgccacgtt tctcgctacg aggacgcgcg ctgtggaaga accttcacgc
      781 cgtgatgggc atctggctcg cactcggcgc actcgcgttc gtgctgagcg ggctgccgtg
      841 gtcggggtca tggggtaagc agttcaaggc gctcgcgacg gccgccaatc ttggcgcgcc
      901 gccgggatcg tggggcggct tgccgttgcg ctccgcactg ccaggcatgc gcaccggtga
      961 acatgcggac acggtggcta caagcgccga gacgcaatca gcgaacggcg tcggcgttgg
     1021 cgttggcgtc ggcgcatcgc acgatgaacg cccggcgcac gcagccggtg atgagcacgc
     1081 cgagcacagc gcgcacgcag cgaacatgga ctcgatgccc ggcatggtga tggacgacct
     1141 gccgttgccg ctcacgccat gggccatcgg caatacgccg ctgccggcat ccgccgatac
     1201 ccatgcggcg cagccgctgc cgctcggacg catcgtcagc ctggtcgcat cgctcggtgt
     1261 tccgaacggt tacgacatcg tgcttccggc ctccgccaca ggcgtctaca ccgtctccta
     1321 cttcccagcc gacccgaaag acgagcgcac gctgtacatc gaccaataca gcggcgcggt
     1381 gctgaaagat atccgttacg gcgactacgg cgcggtgtcg aaggccgtgt cgtacggtac
     1441 gtcgttgcat atgggccgct atttcggcct cgccaaccag attctctgcg ccgcgatttc
     1501 actcggtctg gcgggcatgg ccgtgaccgg ctgcgtgatg tggtggaagc gacgcccgca
     1561 gcgctcgctc ggcgcaccgt cgcgcgagcg cgccgcgccg ccgatgcgcg gctggaaagc
     1621 cggtctcgtt ctgctcggca tcgtcttccc attgatgggc gccacactgc tcgcggtgtg
     1681 gctcgccgac cgcacgattt tcggccgcgc cacgcggcaa gctgcctgaa gcttcattcc
     1741 cgctgtcaaa accaaggagc acatcatgac gacaccacgc aaacttctac tcgccgcctt
     1801 gctgtgcggt tcctcgatgc aggccttcgc gggtgccgcg gcaaccgcga gcgtttcgga
     1861 ctgctggatt cgcgccttgc cgggcgatct gccgtcgggc ggctatttca gggcaagcaa
     1921 tcgcggcgac caaccggtca atctcattgg cgtcgactct gacgcgttcg gcatggccat
     1981 gctgcatcaa acgcagagcc agggcagcac ctcatcgatg gcgatggtcg agcaggtggc
     2041 ggtgcccgcg cacggctcgc tcgtgttcgg accgggcaac tatcacgtga tgctggagca
     2101 tccgaagcag ccgctcaaga tcggcacgac gatcccgctg acgttctcgt tcagcgacgg
     2161 cgagaaagtc accgccgctt gcacggtgaa aagcgcgggc acgatggctc cgtaatgctc
     2221 cccggcgccg ccggacggct gcgaggccgt cgggtcgggc gcgagcgcgg acaaccggat
     2281 gcaggcaatc tgcgcggcga gctttgattg atgcggttcg gccaatcctt gcactactcg
     2341 ctcgtttacg ctacattttg ctcgattgtg atcgttttgt tttgcaatcg agcgaggtca
     2401 ggtcatgcaa cgaacgcgac aggatgcaat ggacgggctt ctgcccgagg aacgggagcg
     2461 gctgattctg gaacggctgc gggcgcaggg ccgcgtcctc gcgtccgaac tcgcggctga
     2521 attccagacc tccgagcaca cggtccgccg ccacttgcgc gaactggccg agcagggtca
     2581 ttgcaagcgc gtctacggcg gcgccctgct gatttcgcct tccggagaga gtgcggcgat
     2641 ccgcctgcgc gaagcggtgg accgcaaggc ctgtctcgcc gttgccgctg cctccatcgt
     2701 ccggccgaag cagatcgttc tgctggacac cggttcgacc aacgccgcta tcgccgcggc
     2761 gctcccggac aacgcggatc tcaccgtcgt gacgaactcg ccggaggctt gcgcacgtct
     2821 cctgaaccgg ccggggttcg acatcattct gatcggcggg cgcatcgtcc ggcacgcggc
     2881 cggctcgacc ggcgccaccg ccttgctgca ggttcagcag atcaaggccg acctttgctt
     2941 cctgggcgcg tgcgcgttcg atccggacga aggcgtcgcc gcgttcgacg ccgaagaggc
     3001 cgaactcaaa cgggcgatgg tcaaggcaag cagccaggtg gccattgcca tgacgtcgga
     3061 aaagctgatg acggctgctc cgttttccgt ggcgccggca tccgccgtcg actatctgtt
     3121 cgtcgaagcg gacgtgacgg ccgagcggcg tgccctcctc cagacagtct gcgacaacgt
     3181 gatggtgacg cgctcatgaa atcactcgcc aagccgtttg ttcagccggt caccaagccg
     3241 ctcaaggaac gtgtggccac gctcggcgtg ttcctcgcca acggcttcgg gatcggcgcc
     3301 tgggcggtcg aagtgccgcg catcaaggaa agcctggcgc tcagcgacgc ctcgttgggc
     3361 atcgcgttgt tcgcctttgc actgggcgcc atcgtggcga tgccgctggc gggacagttg
     3421 gcgccgcgct tcggcagcgg ccgggctacc gcgctgctcg gcgcggcgtt tgtcgtggcg
     3481 ctgccgctcc ccgcgttcgc accgaacctg gcaacgctgt gtatcgtgct cctcgcactc
     3541 ggcgctgcga acggcgcgct cgacgtctcg atgaacggcc acgccagcac gatcgaaacg
     3601 cagtggaagg cgccgatcat gtcgtcgttt cacgcggcgt ggagtgcggg cggcctgctc
     3661 ggcgcggcaa cgggcgcgat gatcgagaaa ggcggtgtgg gcgtgattgg cggtctcgtg
     3721 ctgcctggcg tggtcatcgc catcctcatc gccgccgccg cggtgcttgc gttgcgcgac
     3781 ctcggtccgc gcgcggccgc agcctccagc ggctttgcgt tgcctaatgc cgcggtgatg
     3841 aaacttgcgc tgctggcgtt cctttgcatg ctggtggaag gcgcggtggc cgactggagc
     3901 gcggtgtatt tacgcgccac cttgagtgaa gaagccagcg tcgccgcggt cggttattcc
     3961 gcgtttgcat tgtcgatggc ggcttgccgg atcgtcggcg atgtctccgt gcgccgcttc
     4021 gggtcgagca aggtgatcgg cttcggcgga cttctggcgg cggccgggct cgcgctggtg
     4081 ctgagtctgc cgagcgtctt cacggcttgc gtgggcttcg ccatggtcgg cgtcggcctc
     4141 gcgaatatcg tgccggtcat tttcagcgcg gcgggccgct cgaccgtcac gccggcagtc
     4201 ggcgtctcga tggcggctac ggcggggtat gcgggctttc tggtcgggcc gccgttgatc
     4261 ggattcggcg cgggcttgtt cggcctgcgc ctcgcgttgt gtgtgctcgt cgtggcgacg
     4321 ttgattgtct gcttgaccgg gggtaaggcg gtgcgcaatg cgagccttgc gtagaacacg
     4381 ctgtctgcct gacgagcggc agagcggtgt gcaatgcgag cctcgcccgg aaaaggccca
     4441 tctcgcgcgc gctgcggcca gctcagcgca cctgcagcga atgcgcttgc gcgccatcgc
     4501 tcaggtgctg caaacccatt gtgggtcccc actcagaaca cgccgggaat cacgcggtag
     4561 cgcacctttc ccttgtacgt gcgatagcgt tcgtcgtccg acagcagttg ctcctcgcgc
     4621 acgatgcgca cgacctgcag cgcgaactgg caggcgtaca cgatcacatt ctgcaagccg
     4681 aagttcgtca gcaggaagcc gatgtccgtg atgaagtagc cgagatacat cgggtgccgg
     4741 acgaaccggt acgcaccgcg cgatacaacg ccccgattgg ccggcagaat gccgaacgag
     4801 cgccgcagcg atgccttcgc gaacagttgc cagaaaatcc ccagcacctg caggcacgcg
     4861 ccaacggtct ccgggatcag gtgcgtgccc gggttgagcc gtaccgcgag atagtagtag
     4921 gtaccgccca ttgcgcagat gaacgccaat ggccgccagt cgcgcctgac cggcacgcgc
     4981 gtgatcagcg acaggcccac cgtcacgaac gcggatacca ccagcagcag caacgtgatg
     5041 cgggtgggcg ccgcgcgcca ttgcatcacc gcggcgaagg tgaacccgct gagcatcagc
     5101 accgccgcga cgcgcgatgc gacttcgacc agcgcctcgc gccagcctcc tgcggcttgt
     5161 ccggcagcgt gtggcgccgc ggcagtttgc gaagcgttac ccggcatgat gtcgtcactc
     5221 agtggtgtag tcgttgtcat ggtgcgctca ctgtgaaatg cgaaggattt gcgaggctag
     5281 ttgcgaaaag cacggtgcaa ggtccgtggg tgtcgccgcg tgacaataca cgccgaccgg
     5341 ttgcttcggg ttgtagcagc gcggtagcga atcgggcgta ttggccatgc atttgagaac
     5401 gtcctggccc aactcgttgt cggggcccgc gggttgtact agcggctggc ccaggccgag
     5461 cgtgaacacg tagatattgt tgttgcgtgc gtccgccgcc atcgcttcga gcaggttgcg
     5521 tgaagcgcga ttgacattct gataggtgac ggccgccgtg acgacgcgtg gactattcgt
     5581 gatgatcgga aaggtcgtgt ctgccggatt gtgcgcgttg tagaaggcgg gaagtgacgg
     5641 gatgaatttg ctcgaatcgg ctcgcagatc cgagtcgtag cacggcgaac tgaacgctgt
     5701 ctgcaactga tcgtagcgcc aaagcccggt catcttgccg ttactgtcgg gcgacgctat
     5761 cgtgccgacg aggttattgc acgccttgac cttgttcgta aatgccgacg caaagctatt
     5821 cggcgcacca tcggaaaaga aaacgatcac gcgtagactc gacggatttt gcaactggtt
     5881 catctgattg cgcgcattcc agaacgcttc cgacgaattg gtgctgccgg agaaattata
     5941 tttcccgatt tgcgccgtca tcgtcgcgcg gtcgaaaccc cgggaactat ccttcttgaa
     6001 cggcgtgtcg atgatcgtgc cgtaagcgaa atgcatcagg gcgacgcggt cgttcgtgat
     6061 gtcaaagctg ttcaggaacg agacggcgtt cgcttgcacc acagggggtt cggtcgggtc
     6121 cgttgccatc gacccagtcg tatcgatcac aaacgccata tcgagcgtct tgcggatcgc
     6181 ctgcgaagat gcgccgacgt tcaacacctt gaagccaagc gtctgcatga aggtgaccgg
     6241 cacctgtgcc tgcgccgaca tgttgatcgt cacggtcccc ttgttatcga acacgactga
     6301 gggcgtgcca gccgttgccg tcgaaccgag aaagccggcc ggatagttgg ccgtgaaaaa
     6361 cgcgttggcg gcctgcgtcg cgttcgtggt ctgctccgac tgattgttgc cgcgcgtcac
     6421 ggcctggccc gcggcgacta ccgcgccgtc cgtggcggca tcgagccgcg cccgaatcat
     6481 gtagccgagc cccgaatcga tcgcgaggcc aagaatgccc agcagcgcga ccagcgacac
     6541 cgcgacaatg atgctgatcg agcctttctg atgtttgtat gctgctgttt tcattgcatc
     6601 ccccgacctg cttttatttt caatcagaac accgtcatcg catacagatt cggcgacagc
     6661 gccggtgtcg tgataccgcc gcccagcttc aacgatccga tcagcggtgt ttgcagatag
     6721 aacgcttcga cagcgaacgc aatctgcccg ctcgatagct tgccttgcaa caggttcacc
     6781 accggcgact tggtgcctgg cgcgggcaag ccgctgcaac tgcccgtgcc gtcggtggcc
     6841 caactcgtgc tcgtgccgcc gctgccgccg caggtccaca gtttgctcgc cggcgcgtag
     6901 ttgccgccgt tccagcgata ttgcgcgacc accaccccgg tgcagttgtt gccgttagcg
     6961 tcgcagttgt tgttgcccat gatctcggtg atgtagatca tgccgttcgc gttcatatcg
     7021 agcggtggtg tcgtcgcggt aagcgacgtc atgatcgtct gcatcggata cgtcagcgac
     7081 gcgcgcgacg acaggtttgc cccttcgcgg ctcacgttga tcagaatcat ctgcgcctgg
     7141 atattgcgcg cgatgtcgac gaccggcagc gccagcgcaa cgaggaccgg cagcaacagc
     7201 gccagttcca cgctgacgat accggcggcc ttgcggcggc tcttccccca tggttttgtt
     7261 ttcatgttag aagccctcgt tacgcatcgt cgtcgcgacg ctgaaggtat atttgccgcc
     7321 cgcgaagaac gggctgacca gtggcgtcag gagcggccag atgcaattga tctgcagcac
     7381 cacgatgtcg cccgggttgc cgaacatgcc cgttccgtag ctcgcctgcg tgttgtaggt
     7441 ttgggccgcg ccgccgttga cagtgatcac gtaactcggc gaaacgagcg cgtagagacc
     7501 catcgaactg ttcttgatct cctgcacgat ggccagataa cgctgctgat tggtcgcgtt
     7561 cggatcaaga ttgctttgcc cggtcaccgc gtaacgcgcg ccttcgcgca ccgcgtattg
     7621 catcgtcaga ttgacccaca acgcaatccc catatccatc acaatgcaca gcagcagaaa
     7681 cagcagcggt gccagcagcg caaactcgac cgtgacgacg ccacgctgtt tgagcgttcg
     7741 ctttggcgct tgcttcaatt gcgggcgagc agctttcata tcgcctcctc gttaagtgtc
     7801 accaggccgc gtctaacgga ccgtcactcg gccgcgcgct gcgtcgcggc gacgggttcg
     7861 cccgcggtgc gcttgcgcag tggccaatac agcgctacca gtaccgatgc gggctcggca
     7921 tcgtcatcga ggccggtttg cggccactcg tgcggtgccg ggacccacat cgtgccgaac
     7981 agacggtccc acagcggcag cagttgcgtg aagtttttgt cgaagtgttc agtgcggcga
     8041 gagtgatgca gccggtgata ttgcgggttg ttgacccacg tgatgaagcg gccgaggtcg
     8101 atgcgcaggt tcaggtgggc aaaaaaatta cccggcaaaa aaatcagggt ggccacgcca
     8161 acgatcgacg gctcgacctt gaatagcacg cctaccagtg gatacatcac gcagcccttg
     8221 agcagcgctt caaaccagta gtgacgcgtg gtcacagtga cgttgaagga ctccgcgctg
     8281 tgatggaacg aatgcatgcg ccacagcagc ggccacgcat gctgcaaacg atgaaaccag
     8341 tattcgagca ggtcgatcgt gagcactacc gcaagaatcg ataccagtac gccccagccg
     8401 cgagcaggca atgcaaacag gcccccgccc agcagattga cgatccagac cgacgcggcc
     8461 gccgtcagcg gctgggtcgc ccggccgaaa gccgtagaga tggccgagta agcgaggttc
     8521 agacgggtgg ccgtccggga ctgcagacgc tccacaggcc agcgcctctc gagcaacgcg
     8581 ccgacgatga acaacgccaa cacgatctca taggtgagcc accattcgtg cgcgaagtgc
     8641 catagaacag gaagcggatt ggactcaaac atgtttttcc ccggtagaga caaggccggc
     8701 atggtgtcta gccgccctag atgccgctca tcgccggcac cagctggcgc atcacctgga
     8761 ttgccgacgg gccgatcaac acggtaatca atgcgggaaa gataaagaac atcagcggaa
     8821 agagtaactt gagcgcgatc ttggcagcct tctcttcggc gcgcatgcga cgtttggtgc
     8881 gcaggttgtc tgtatagacg cgcagcgaat caccgacgct ggtgccaaag cggtcggcct
     8941 gaatcagcat cgcagcgagc gtgtcgatgt cttcgacgcc ggtgcgtagc gacaggttgc
     9001 gcagcgcctt gtcgcgcccc gaccctgcgc gcagttcgag cagcaccaat tcgaattcgt
     9061 ccttcagcgc gtggctcctt acaccgattt cctgcgtgac gcgcatcatg gccgcgtcga
     9121 ggccaaggcc ggcctccaca cagacggtca tcagatcgag cgcatcgggc agatcttcga
     9181 acagcttgcg ctgccggtgc tcgatcatgc gtgaaaggat caggttcggc agatagaagc
     9241 cgagtgccga cgctgcgagc accgcgagca gcagccacat gcgccgctca ggggtgaaga
     9301 gattgccgca aaaaatcagg gcgatcgccg gcaacaccag cgccaatagt gtcttcgcag
     9361 cgaaataaag cgccggggcg gagggattgc ggatgcctgc tttggcaaac tgcagccgga
     9421 gcgtcgactt atcccaatcc tctttcggca aagaaagctt tgcgacatgg tgggacatct
     9481 tcgtgacggc ttcgacccac gtcttctgtt cgcccgagtc ggactcgtcg ggggacgtag
     9541 gtgcgccgac gcgatcgata cgccgttgca agggatcggg ccgcagcagc atcatcgcct
     9601 tcagcatggc gccgaacacg atgataaaca tcagggccag tacgacgatc tgttcggttc
     9661 tcatggtctg cgggttgccc cgccctcctc gattcgttgt tgcctggtgt gccggcttga
     9721 aatcgttctg tcctgttcgg cgactacacc cggatacgta tcaccttgcg catccacaag
     9781 atgccgaaca gcatcatcgt gagcgcggac atgccaagac ggataccgag cggatcgcgc
     9841 cagaacacct tgatatatcc cgggttgagc agcgacagta cccccatgac ggcgaacggc
     9901 agcaccgcaa gaatccacgc cgacagccgc ccctccgccg acagcacacg caccttgccg
     9961 agcagcttca ggcgctcgcg aatgatgctg ctgatgctgc caagaatctc tgcaaggttg
    10021 ccgcccgcct cgcgctggat cagcacggcg atgacgaaat agcgcaggtc gtccaccggc
    10081 acgcggttca ccatgttcag cagcgcatcg ttcatcgaca cgccgtaatt gatctcatcg
    10141 aacaccagtg cgaactcccc gcctagcgga ttgggcagct cttcgccgac catcccgagt
    10201 gcagccggaa acgagtggcc ggcacgcagc gcacggctaa tcagatcggc ggcgtccggc
    10261 agttgccgtt caagctgcaa cacacgcttg ccgcgcttgt ggcgcaggat cagcaacggc
    10321 agcagcgcgc tcgcgccggc gagcccgagc atcaccgtcc atggcgcatg cagcacgaat
    10381 ccgatgaaca tcccaataag cgcggacagc agcgtatagg tcacaaaacg cgcgacggac
    10441 cacgtcaatc ccgactgttg cagttgccgg tcgagcgtat gcacgcgcgg caaaaccagc
    10501 agcagccgcg tgaccagcgg cgattcgctc agcaggcgct gcttcagaat cgacagttgc
    10561 tccgcgctca cgttgccgcc cgcggacaac gcgcggatgc gctcgtccat gcgcttgacg
    10621 acggggctgt gatggctgct ccagaacaga tagatgctct cgatcagcag cacgatcgcc
    10681 agaaacgcca gaaccgcgaa accgataaac gatgtgttca tgctcgactc cttggacgac
    10741 gcggaccgtg tggatcgcgt cgtgtgctta cacgtccaga cgccgcgcgg gatcgaacat
    10801 ctgatccggc agcgtgatac cgaagccggc cagccggtcg cagaacttcg gacgcacacc
    10861 ggttgcgcag aaataacctt tgatcatgcc gttctcgtcg acgccgatgc gcttgaacgt
    10921 aaagatctcc tgcatgttga tgatgtcgcc ctccatgccg gtgatttcct gaatgctcat
    10981 caacttgcgg cggccgtccg tgagccgcga cgcctgcacg accaccgtga tcgccgaggc
    11041 gatctgctgg cgaatcgcct tgatcggcat cgtcaggccg gccatgccga ccatgttttc
    11101 cagtcgcgtc aaaccatcgc gcggcgtgtt cgcatgcagc gtcgccatcg agccttcgtg
    11161 gccggtgttc atcgcgtgca gcatgtcgag tgcttcggcg ccccgcactt cgccgagcac
    11221 gatccgatcg ggccgcatcc gcagcgcgtt gcgcaccagc gagcgctgcg aaatctcgcc
    11281 cttgccttcg atattcggcg ggcgcgtttc gaggcgcagc acatgttgct gacgcagttg
    11341 cagttccgcc gcgtcttcga tcgtgacgac ccgctcgtcg tcgggaatga aaccggacag
    11401 cagattcagc aaggtcgttt tgccgctgcc cgtgccgccc gaaatcagca cgttgagctt
    11461 cgacttgatc agcgcttcga gcagttgcgc catggccggc gtgaagctct ggttgttcac
    11521 catgtcggtc acggtcagcg gattcaccgc gaagcggcga atcgatacca gcggaccgtc
    11581 gatcgccgac ggcggaatga tcgcgttgac gcgcgagccg tccggcaggc gcgcgtcgac
    11641 catcggcgtc gattcgtcga tgcggcggcc cacccgcgag gcgatccgtt cgatgatctt
    11701 catcagatgc gcatcgtcgt agaaggtcac gtcggtgtgt tcgaggcggc cgcgccgttc
    11761 gacgtacacg tgttgcgacg tgttgaccag aatgtcggaa atggtcggat cgttgagcaa
    11821 cggttcgagc ggcccgagtc cgaccatttc gtcatgcacg tcctgcgcga gccggcggcg
    11881 ctccagctcg ttcatcggaa tcttgtcttc ctcgacaata cgttcgacga gttgcgccag
    11941 ttcgcgcttg acctgctcag gtgaaaggcg ttgcagcttg tccagttcga cgcgctcgat
    12001 gatcgtttga tgaatagtca tcttgagctg ctggtatgcg cgccgtgccg caaggctctc
    12061 ggcggcgaca cccgccgcag cggcgcccgc cgtttctgcc ggcataaatc cgttctggat
    12121 cgacatctgt tcgcgcagtg acatgatggt ctccgttgcc gttcgcaggt cagtgttcgg
    12181 tcttcaacgt ttgaagggaa ttcggccgag ccgcgaagaa gcggctcagc acgcctttgc
    12241 gtggtgcggc ggcgatcggc cacagcatcc cggcgagcgc gccgatgcca agggccagcg
    12301 cactgccctt ggccgccgtg acaaccggta tgccgtgatt gagcgcgtcg ttcgcgtgtt
    12361 tttcgtcgcg cggcaactga tgcgcgacct tggcgccaag cgtttcctcg agtgtcgcga
    12421 ggctgatgcg ggcgttcttg tcgtactggt tcacgatcag ccgcaccttg ctcgccgaat
    12481 acccgagttc cttgaagata tccagcatgc ggcgcccgga gtgcagatag agaatgttct
    12541 gccgtatcac catgcaaatc gagtcgctgt gatcgagcac atgaatcgcg agcgggttga
    12601 tactctggcc gacatcgacg atgaccgcgt cgtattgctc cctcaccagc atgagcatcc
    12661 gctcgagatg cgcgggacgc agttcggctg ccttgaccgg atcacccgcg ccggccagca
    12721 cgtccaggtt tgcgtgaatg tgcatcacgc aagcctcgaa gaaagcggca tcgagccggt
    12781 cgatctgcga acacagatcg gtcagcgtcg cgcgcggcgc cttgtcggcc accagcaggc
    12841 tcgcgtcggc gaactgctgg ttcagatcga tcagcagcac gcgcttgtcg cgctgcgcag
    12901 cgagggtgta agcgaggttc acggcgatca gcgtggcccc gctgccgccc ttgcacgaac
    12961 tcaccgatac cacgcgccct tcgcggcgca ttcctgcgtt tttcttgttg gcgacatgcg
    13021 tgagcgcgtc gacgatttcg cgttcgtcca gcggccacga caacacgtga cgcacgccaa
    13081 cgcgcatcgc agccatgagg aggttggtcg acggtgcggg ggtgatcagc atgcaatgca
    13141 gatgcggcgt ctggaccagc gcctcctcga tgccgctcag ttcgagctcc gacagatcga
    13201 cgtcgtcgac gatcagcaga tccgcggtct tgatcgccgc ggcgtgcgcg cgcaattggc
    13261 gcgcctcgcc atgcacgacg cgcagccggt aggcgacgcc gcttgcttcg agccgcgcgc
    13321 cgatatgcgt cgagcgttcc gcgctggacg aaatcatgag gatgtcaatc atttcaagtc
    13381 tccgctgacc tgcaaggtgc tgttgccaga acctctatca gtaactactg cccgagccga
    13441 cgccgatcgt gaacatattg gttggcgggg tcggcgcggt gaacgatttg gcgtagccct
    13501 tttgcgccgc cgccgccgcc gtgccgtcga tccccaaaac cggatcggta ttggccgagg
    13561 cttccggatt gagtgtctgc atcgctttga gctgattgac cgaatcgccg aaggtcgcgt
    13621 cccagtgggg tgtcgaggtg aggcacccgg cgagcgtcgc agtcacgcct gcgcatagcg
    13681 cgaggcggat cagtgtggga atggctttca tgtcgttctc cgaataaccg ttagatgcgc
    13741 gcctggctca tcgcgccgcg tgtgagtcgg cgtcagccgg aagcgccgcg ctgacgactg
    13801 ccgcggcggg gaccggttcg ctatgcggcg cgtcattggg tttggtcgac gtgagcggct
    13861 gcgactcggg cgtgccgtgg ttgtcgtccg gcgcgggtac gcgggcgggc ccggagaccg
    13921 gtaccgggtt gcgatcaggg gcaggtgccg gttgcggcgg cagcgtgggc gcagccagca
    13981 cgggcgacgc cgctgtagcg cccgatgagg ctcccgcagc aggcgccgca tacttcttgc
    14041 cttccatgtt gccggtcaga tagacgttgc cttcggccgc gtcgacgaaa ccgtcggtcg
    14101 gcagcggata gttttgcggc aggggtttcg ccaggcgcgg cgtgaccacg aacaccagtt
    14161 cggtcttgtc ctgttcgtag ttcgtgctgc ggaacagcgc gccgagcacg ggaagttcgc
    14221 ccactcccgg cacgcccttg agcgtaccgg tcacgttgtt tttgatcaga ccgccaatcg
    14281 cgaagctctg cccgtcgtac acctgcagcg tggtcgaggc gcggcgggtt gtgatcagcg
    14341 gcaggatcgt cgtgttattc gagttgccgg cgctcaccac cacgccggtc gacgacagct
    14401 cggacacttc cggcgcgacc ttcaggttga tgcggccgcc ttcgagcacg gtcggcgtaa
    14461 aggtcaggcc cacgccgtat tgctcttcct gcagcacgat cgtcgtgccg ttcgcgccgt
    14521 tgctttgcgg caccggaatg tagaccttgc cgcctgccag gaagctggct tcctggccgc
    14581 tgatcgccat caggttcggc tcggcgagga tcttcacaag ctggtcggtt ttctgggcgt
    14641 cgaacgcaag attgagcggc gagttattgg ccttcgacgc tgtgattgcg ctgagcccgc
    14701 cggacagata attcgccagc aagccaaagc tccagctgcc gatcccgccg ttgagattgg
    14761 cagccatgcc caactggtcg atcagtgtct tcgacacttc ggcaaccttc acctcgagca
    14821 tcacctgctg aggcgcggcg atgtgcatca tattgatgac gcgttcggaa ttgccgcttc
    14881 ccaacgtcgg cggcggcggc atggcgacac gcacgccgcc gatcggcagc ggccccgcag
    14941 ccggcgcggc gctcggcacg gccacggtag gccgcgccac gaacgcgtgc gcgagctcga
    15001 cgatgcgttc agccttcacc gaatccgata ccacgccggt cagcaccagc gtgtcggcgg
    15061 cggctttcac gcgcacgccg gtttcttcgg gcatcagcac cgcgagtgtc tgttgcaggc
    15121 ccgccgggtc cgcgccgaca ttcacgtcga tgatcgtgca cgagccgctc ttgccctgca
    15181 cgatcatgtt ggtcgtgccg acgtccgagc cgagcagata gagggtggtc ggcgacacca
    15241 gcatcgcctg cacgatcgac gggttgccga ccgtgcgggt acgcaccggc tcgcgcagat
    15301 cgaccatggt cgacttgccg accggaattt gtaccgacgt atggtcagcg accggccccg
    15361 tgcagctcgg gcctttgccc gcttgcgacg agccgttgct gggcgcgccg ctgccgctca
    15421 tgccgatggt cagatgcatg gggccgttgg ccgcgacggt gagcggcgtg tccggcagcg
    15481 ccggcacgtt ctgcgcggca gcctgcgtgg cgggcagcgc ccacaggcag gtgccgatca
    15541 atccgcatgc gctcgcctgg gcgatccgca accgggtcct gagttgcgtg acgtgcggag
    15601 cactcgccgc atgcgcgcga tggttcggtc tggcgttcat gtcctacccc cgtaatggcg
    15661 ccgtttgtca cggcttgcag gctgagcctg tcaaaagcat tcttgtcttg aatctgcacc
    15721 gtccagcacc cgcacgcagt tgcccggcgc ccgcgccgcg actttccgca cgatgtgcac
    15781 tgccggtgcc ggtgccgatg caggcgccgc cggtacggcc accggaatct tcagcagcga
    15841 tgccttggtg gcaccactcg tatcggtagc ttgtggatcg acctgattgc gcaacaccag
    15901 agaaagcgtg ccgatgctgc gcgcgagatc gatcttctcg gcctgctcgg gcgacacttc
    15961 gagcgtcacc gcgtcgacga ctttcggttt ggtctcgtcg cgcccgactt cctgcgcgac
    16021 cgccagcacg agaatctttt cgagcacgat cttcgagatg ttctgatcgc gctgcgtgcc
    16081 cgggtctttg ctgtctttct gtgtattgac gatgatgtcg acgtagttgc ccggcaacgc
    16141 gaagccggca acgccgatca catcgttcac ccgcaccgtg atcgcgcgct tgccttcatt
    16201 gatcaccgcc gacaacccgc cgagcgtgcc gaccggcgag agcttcgcct cgaggatcgg
    16261 ctcgccgtgc aacaggcttt ccttcagcac gcggccgtcg agcttctgca tgtcggagaa
    16321 cgaacccggc ggcacactgt tcgacggcca gtccgcgacc ttgaggaaat caggcgagat
    16381 gcgttgaccc agattgatgt ccgacacggc cacgacgact tttgtcgtcg cgctcgaggt
    16441 ctgcgacgtc atccagcgcg ccgcgaagac caccgcggtc aggccggcca tcgtcgcgat
    16501 cagcagcatg attaaagcgc gggcgttttt catttcgttc tcctgcatcc tgtcgtcgct
    16561 cgacgacgct atgagaatgg cgctaccgca gccgatgacg gcgtagtcag aaccgtgcgc
    16621 tgcaccttcc ctggtctacc tcgtcacacg ctcgcgaata acacgaccgt gctgcctatc
    16681 gctatcgcta caccgtacgg tatggttccg atcgaaccgt gtgcgaggcc gctgcgtcgc
    16741 tgcgctgtgg cgtcaccggg cacagcacgc tcggccagca gtgagcgggc cccaatcaat
    16801 cccgtcaaca tctggcgccg cctgagcaac accgccagcg accacgcacc acccatcgca
    16861 aaggtcgcca gtccaatttc gaatgcgccg tatgcgccgc agaacgcacc aacagcggcc
    16921 atcagcttca catctcccgc acccatcaat cgcatcagat aacccggcag gagcagcagg
    16981 ccacccgtca cacaaccacc caaccacatt tgcatccctt cggctgctcc gtgaagcacg
    17041 acctgcaccg gcagcgcgac gacgagcgcc gtggcaatta gccagttcgg aatcctccgc
    17101 gtgtgcaggt ccgacaacgc cgcgatcatg acgagcgcca acacacaagg tccaatggga
    17161 aagggcacac cgttcatctc acgctccgtg tcaggatcga cgcagttcgg gatcgagcca
    17221 gccgtcgctt tacctcaaca tgcttgatga gataaagcag acgaccggac tcaccgatca
    17281 cttcagtccg cagcgatcag gccgggatgg cgatgcccgt gagcttattc ccgatgttgt
    17341 tgaagagatt ggaaatgtcc gcgcccagcg cggccgacgc gacgataatc cccaccgcaa
    17401 tcagcccggc cagcaggccg tattcgatcg ctgcaacacc gtcttcttcg cgcagaaatt
    17461 gttggatcgt gttcttcatg ctaacccccg taagagtgaa agttgacttc ggtttgcgcc
    17521 gccgcagttg cctgaaaaca ggctcaccgg agcggtgttc aggtccgcct atagagacgg
    17581 aaaaagtggc gaggtcagcg cttcacgtcg tcccggtcgc gaatggccga taccccgtat
    17641 cgatcattcg cgccgtgctt tctcgacatc cgcttttcgt tggccccgcc gcaccccgca
    17701 aagcgcgttg atttccccga cgcgtcttta cgaagactgt tgttgctacc gggatttctt
    17761 cctgctgctg gttcgtttgc tgcttcccgg ccgcaacgtg tttcgctgtg tgactcgctt
    17821 atcgcacggg ctatgccatg tgccctcaaa gccttgccgg acggcgattt gagatgcggc
    17881 ggaccttgca aacggggatt cgcatcgtgc gatgtttcgt gtttgagacg ccgcggatgg
    17941 cggtcgccgc gggccggcgc gatgtgccgc ggatcacccg cgaatgaagg aaaaccctaa
    18001 gcctcgctga ctggcaatgg tttgacgaat actccggtca aacatgccgc gacgtcgtgc
    18061 caagaagtag cttttgcggg ccgtggaccg acatgtcgca accttgagac gaaaagccgg
    18121 cgtagaggcg gatctgtcgc gggattgaga tggccggctc atgcatcgct atgtgcgctg
    18181 gcccgcatta ccgggattga cggaggctct ttaaatcgcc tggacgtggg ccagggaacg
    18241 cgcccgacgc tttttttttg tgtgacgcaa gcggtgcgtt cgcttcgact cggacgaa
//
LOCUS       DATASH010000020         7660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_793851,
            whole genome shotgun sequence.
ACCESSION   DATASH010000020 DATASH010000000
VERSION     DATASH010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7660
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793851"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="VHV99_00565"
     CDS             complement(<1..220)
                     /locus_tag="VHV99_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEX3633990.1"
                     /translation="MTSIRRWLLGWLIFGLAAASLVAAYGIFHTARQEASELFDYELR
                     TVALSLPSNLAGDGDGEHRGPDFGDLADD"
     gene            complement(217..879)
                     /locus_tag="VHV99_00570"
     CDS             complement(217..879)
                     /locus_tag="VHV99_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3633991.1"
                     /translation="MRLLLVEDDEMIAETVSGALRRSGYAVDWAEDGRAAELSLGNDV
                     YDLVLLDLGLPKKDGIDVLNAYRKNGGAAPVIILTARDAVDERIRGLDTGADDYLIKP
                     FDLDELAARVRALLRRRTGQKQPVYAHGGLTLDPAAHEVTKDGEPLPLVPREFALLQA
                     LIEEPTRVFTKAELEDKLYGWGEEVGSNTIEVHVHSLRRKIGAEQVVTVRGVGYRLKR
                     CG"
     gene            1098..2534
                     /locus_tag="VHV99_00575"
     CDS             1098..2534
                     /locus_tag="VHV99_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX3633992.1"
                     /translation="MPVFHAAVAAPSAVRLRALAPMVCALLLAGCTWYHREPLAPQDT
                     STSARSLERVRIDASTMPLPELAAHRFDPSDGLDIDEVAMLAVANNPELKLARDDLGI
                     ARAQAYSAGLLPDPQVSVSSDYPGAIGTTRAFSYGLSMDVMAIVLRSANRQSADASVA
                     RTDLGLLWQEWQIVAQARQLFIKTRFQQDTLPLLQQQRDLARTRYERMAEARRDGNLT
                     DDTLTAALTSYSDARKQYTDAERAAEQTHHDLNALLGLSPEVEVQLQAATPGGGDDPA
                     DLSDATLDAALSHLAQRRPDLIALQAGYEAQEQKYRAAILSQFPSLSVGFVRARDTSD
                     IYTSGFQINLSLPIFNRNQGNVAIEKATRQRLRDEYQSRLNQAYADVAHLRADNAILT
                     RQLHQTEAALPDVDIAAQHAAAAYAEHNLTLGAYTDAQSASLTKRIDVATLRESLAEQ
                     RVGLQALLGSAIPDAFSSAQTYTDTHAK"
     gene            2524..3642
                     /locus_tag="VHV99_00580"
     CDS             2524..3642
                     /locus_tag="VHV99_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX3633993.1"
                     /translation="MRNSPFPARRPRIASLALSVACATLMSAAVHAAGAPAADDTADR
                     TVVSVQTVRVQRAVIAQPVRAYGIVAASASNLTTVNLPYVARIVQMRVQAGQSVTRGT
                     PLCVVQADPAAVLAATQAKSAVTLAQGELARTQSLYDKGLATQSQLASAQKAVEDARQ
                     ALAAQNEEGVTRGNKTIVAPLDGVVLQVSAAQGDQLQPGAAILQLAGGTGRDARANVM
                     LGVEPADASAIHPGDTVTLHGLSASLAKAATEGHVVLVGASVDQQSQLVNVGANVPLG
                     QSAFIPGSRVAADIATRSGTHWVVPRSAVLKDDKGAYVFQITPQNKARRVAVITQIEN
                     GDRYGVDGPLDSAQGLVVSGNYELKDGMAVQAGGGAPR"
     gene            3639..6686
                     /locus_tag="VHV99_00585"
     CDS             3639..6686
                     /locus_tag="VHV99_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX3633994.1"
                     /translation="MNFGQWMQKHRRSLLFVIALLAIAGALTAFRLPISLFPNVAFPR
                     AVVSLDAGDRPAEQMATLVTMPVEEALRRVPNVRDVESKTSRGAAEISLNFDWGTDMA
                     QATLQAQSAISEILATLPQGTSMQVRRMDPTVFPVLAYSLTSNQQSLAALHDLAQFQM
                     RPLLSSVEGVARVDVTGGDQDEFEVAIDPQQLAAYKLSLADVSKAIGASNVLMANGRI
                     EDHDKLYLVIANTTITQLNELRNVVVSASGAAQIRLGDIATVRQGIVPQWMRVTADGQ
                     DAVLLNVYQQPGANSVAMAKAIRAKLAGFQPQMPAGVHLSNWYDQSELVIASASSVRD
                     AIMIGVVLAALTLFAFLRNWKITAIAVALVPVVMAATILLLDVFGMGFNIMTLGGMAA
                     AVGLVIDDAIVMIEHIVRRMREGGAHAFHGRVMAAALEFTRPLAGSSAATLIIFVPLA
                     FLSGVTGAFFKALSVTMASALFISFLVTWLAVPILCDRWLKPKDAEEHKDTRFAAWMN
                     KRYAYLVERVTARPILVLLGLVPLILVAAFAFTRVGSGFMPSMDEGGFVLDYHTEPGT
                     SVTETDRLMRQIERIIRANPNVATYSRRTGAGLGGDLNEPNKGDFFIRLKSSGREPIE
                     TVMEEIRSQIETQVPGVSVELAQLMEDLIGDLTAVPQPVQIKIYSDDPNTLDTTARKV
                     AARIGKIQGVVDVNDGINPAGDALELHIHPEAAAAEGMDPQSIAQAVTDMVEGSVATQ
                     FQRGPKTVGVRVRVAGAAKLTDTQLGQLQIRAPDGHLFALNRVADQVTVTGQPEISRD
                     NLKRMVAVTARIDGRDLGSTIADVQKTMSDRSLLPAGVYYELGGLYQQQQIAFKGLLT
                     VFGAAIALVFGLLLFLYERFRVALAVMAMPLLAAGAVFIGLWITGIELNISAMMGMTM
                     IIGIVTEVAIFYVSELQGLITDEGVPFAEALLAAGRNRLRPIAMTTIAAILALLPLAF
                     ALGQGSAMQQPLAVAIISGLIVQLPLVLLLLPVLLKLLMRRAV"
     gene            complement(6789..7385)
                     /gene="tatB"
                     /locus_tag="VHV99_00590"
     CDS             complement(6789..7385)
                     /gene="tatB"
                     /locus_tag="VHV99_00590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HEX3633995.1"
                     /translation="MFDFDISKLAVIGTVALVVLGPERLPRVARTAGTLLGHAQRYLN
                     SVRAEVDQQIRIDELRKVKDDIERAVTHVQDTVDRTVRQHASELQADVDAAAASLRDA
                     LPASYLPSVHEGGGNFQADLFKVRPAQLQMSGSSDASIHAYRPDAVSSSAPASAVQHT
                     RARWRGSVSGSRSAAVKRTRVVSMAASKALGRDGGRLA"
     gene            complement(7501..7659)
                     /gene="tatA"
                     /locus_tag="VHV99_00595"
                     /pseudo
     CDS             complement(7501..7659)
                     /gene="tatA"
                     /locus_tag="VHV99_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434498.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
BASE COUNT         1349 a         2549 c         2439 g         1323 t
ORIGIN      
        1 ggtcatcggc cagatcgccg aagtcggggc cgcgatgttc gccgtcgccg tcgcccgcga
       61 gattcgacgg caacgagagc gcgaccgtgc gcaattcgta gtcgaacagt tcgctcgctt
      121 cctggcgcgc cgtgtggaag atcccgtacg cggcgacaag cgaggccgcc gccaggccaa
      181 aaatcaacca gcccagcagc cagcggcgaa tcgacgtcat ccgcacctct tcagacggta
      241 gcccaccccg cgcacggtca ccacctgctc cgcgccgatc ttgcgccgca ggctgtgcac
      301 gtgcacttcg atggtgttgc tgccgacctc ctcgccccag ccgtacagct tgtcctcgag
      361 ttccgccttg gtgaagacgc gcgtgggttc ctcgatcagc gcctgcaaca gcgcgaattc
      421 gcgcggcacg agcggcagcg gctcgccgtc tttggtgacc tcgtgggccg cggggtcgag
      481 cgtgagtccg ccgtgcgcgt agaccggctg cttctggccg gtgcgccgcc gcaacaaggc
      541 gcgcacgcgc gcggccagct cgtcgagatc gaagggtttg atcaggtagt cgtcggcgcc
      601 ggtgtcgagg ccgcgaatcc gctcatccac ggcgtcgcgc gcggtcagaa tgatcaccgg
      661 cgcggcgccg ccgttcttgc ggtaggcatt gagcacgtcg atgccgtctt tcttcggcag
      721 gcccagatcg agcagcacga gatcgtagac gtcgttgccc agcgacaatt ccgcggcgcg
      781 gccgtcctcg gcccagtcga cggcgtagcc cgaacggcgc agcgcgcccg atacggtctc
      841 cgcaatcatc tcgtcgtctt cgaccagtaa cagacgcatg gcgtctcctc gctccagttt
      901 gcccgcattg tccggcctgc cagcttaacg tttccttagg cgaacccgat gaaacgccga
      961 tgcgcggccg aagcgacgat caagggacgc tggcgcggca gttaagcgtt tcctaagtat
     1021 ctcatccgca gaatcgattc ctgtcgtgcg gtatcccgtt cgcggctgcc gcgttctttt
     1081 cgttcaggag ccggattttg cccgttttcc acgccgccgt agcggccccc tcagcggtac
     1141 ggctgcgcgc gctcgcgcca atggtctgcg cgctgctgct ggccggctgc acgtggtatc
     1201 accgtgagcc gctcgcgccg caagatacgt cgacctccgc gcgttcgctc gaacgcgtgc
     1261 gcatcgacgc gtcgaccatg ccgctacccg aactggccgc gcatcgcttc gatccgtccg
     1321 acggactcga catcgacgag gtcgcgatgc tcgccgttgc caacaatccc gagctcaagc
     1381 tcgcgcgcga cgacctcggc atcgcgcgcg cccaggccta ttcagccggc ttgctgcccg
     1441 acccgcaagt gagcgtctcg agcgactatc ctggcgccat cggcaccacc cgtgcgttca
     1501 gctacggctt gagcatggac gtgatggcga tcgtactgcg cagcgcgaac cggcagtccg
     1561 ccgacgcgag cgtcgccaga acggatctcg gcctgctctg gcaggaatgg cagatcgtcg
     1621 cgcaggcgcg gcagttgttc atcaagacgc gcttccagca ggacaccttg ccactgctgc
     1681 aacagcagcg cgacctcgcg cgcactcgct acgagcggat ggccgaggca cgccgcgacg
     1741 gcaacctgac cgacgatacc ttgaccgccg cgctcacgtc gtacagcgac gcgcgcaagc
     1801 aatacaccga cgccgaacgc gcggccgaac agacgcatca cgatctgaac gcgctgctgg
     1861 ggctttcgcc cgaggtcgag gtgcaactgc aagccgcgac gccaggtggc ggcgacgatc
     1921 ccgccgacct gtccgacgcc acgctcgacg ccgccctgag ccatctcgcg caacgacgcc
     1981 ccgacctgat cgccttgcag gcgggctacg aagcgcagga gcagaaatat cgcgcggcga
     2041 ttctcagcca gttcccgagt ctgtccgtgg gcttcgtgcg cgcgcgcgat acctcggaca
     2101 tctacaccag cggcttccag atcaacctga gcctgccgat cttcaaccgc aatcagggca
     2161 atgtcgcgat tgaaaaggcg acccgccagc ggctgcgcga cgagtaccag tcgcgcctga
     2221 accaggctta cgcggacgtc gcacatctgc gcgcggacaa cgcgattctc acgcggcaat
     2281 tgcatcagac cgaagcggcg ctgcccgacg tcgatatcgc cgcgcagcac gccgctgccg
     2341 cgtacgcgga acacaacctc acgctcggcg cttacaccga cgcgcaaagc gcttcgctca
     2401 ccaaacgcat cgacgtcgcg acgctgcgcg agtcgctcgc cgaacaacgc gtcggtttgc
     2461 aggcgctgct cggcagcgcg atacccgacg ccttttcttc cgctcagacc tacaccgaca
     2521 ctcatgcgaa atagcccttt tccggcgcgg cggccgcgca tcgcctcgct ggcgctctcc
     2581 gttgcctgtg ccacgctgat gagcgccgcc gtccatgccg ccggcgcgcc tgccgcggac
     2641 gataccgctg acaggaccgt cgtatccgta caaaccgtac gcgtgcagcg cgccgtcatt
     2701 gcgcagccgg tgcgggccta cggtatcgtc gcggcgtccg cgtcgaacct caccaccgtc
     2761 aatctgccgt acgtcgcgcg tatcgtgcag atgcgcgtgc aggccggcca aagcgtgacg
     2821 cgcggcacgc ccctctgcgt cgtccaggcc gatcccgcag ccgtgctcgc ggcgacccag
     2881 gcgaagagcg cggtgacgct cgcgcaaggc gaactggcgc gcacccagtc gctgtacgac
     2941 aaagggctcg ccacccaatc gcaactcgcg agcgcgcaga aagccgtcga ggatgcccgg
     3001 caggcgctag ccgcacagaa cgaagaaggc gtgacgcgcg gcaacaaaac catcgtcgcg
     3061 ccgctcgacg gcgtggtgct gcaggtgtcg gcggcccagg gcgaccagct gcagccgggc
     3121 gccgcgatcc tgcaactggc gggcggcact ggacgcgatg cgcgcgcgaa cgtgatgctc
     3181 ggcgtcgagc ccgcggacgc cagcgcaatc catcccggcg acaccgtcac gctgcacggc
     3241 ctgtcagcct cgctcgcgaa ggccgcgacg gagggtcatg tcgtgctggt cggcgcttcc
     3301 gtcgatcaac aaagccaatt ggtgaatgtc ggcgcgaacg tgccgctcgg acagagcgca
     3361 ttcattccag gctcgcgcgt ggccgccgat atcgcgactc gcagtggcac gcactgggtg
     3421 gtgccccgtt cagcggtgct gaaagacgac aagggcgcct acgtgttcca gatcacgccg
     3481 caaaacaagg cacgtcgcgt ggcggtcatc acgcagattg aaaacggcga ccgctacggt
     3541 gtggatggac cgctcgacag cgcacaaggt ctcgtcgtca gcggcaacta cgagctgaag
     3601 gacggcatgg cggtgcaagc tggcggaggc gcgccgcgat gaatttcggt caatggatgc
     3661 agaagcaccg gcgctcgctg ctgttcgtga tcgccctgct ggcgatcgcg ggcgcactga
     3721 ccgcgttccg cttgccgatc tcgctgttcc cgaacgtcgc gtttccgcgt gcggtcgtgt
     3781 cgctcgacgc gggcgaccgc cctgccgaac aaatggccac gctcgtgacg atgcccgtcg
     3841 aagaagcctt gcggcgcgtg ccgaatgtgc gcgacgtcga gtcgaaaacg agccgcggcg
     3901 cggcggaaat ctcgctcaat ttcgactggg gcaccgacat ggcgcaagcc accttgcagg
     3961 cgcagtcggc gatcagcgag attctcgcga cgctgccgca aggcacgtcg atgcaggtgc
     4021 ggcgcatgga tccgaccgtg tttccggtgc tcgcctatag cctcacgtcg aaccagcagt
     4081 cgctggcggc actgcacgat ctcgcgcaat tccagatgcg gccgctgctt tcctccgtgg
     4141 aaggcgtagc gcgcgtcgac gtgacggggg gcgaccagga cgagttcgaa gtcgccatcg
     4201 atccacagca actcgccgcc tataagctct cgctggcgga tgtctcgaaa gcgatcggcg
     4261 cgagcaacgt gctgatggcg aacggccgca tcgaggatca cgacaagctg tacctcgtga
     4321 tcgccaacac cacgatcacg caactgaacg aactgcgcaa cgtggtggtg tccgccagcg
     4381 gcgcggcgca gatccgtctc ggcgatatcg ccacggtccg tcagggcatc gtgccgcaat
     4441 ggatgcgcgt caccgccgac ggccaggacg ccgtgctgct caacgtctat cagcaaccgg
     4501 gcgcgaacag cgtggcgatg gccaaggcga ttcgcgcgaa gctcgccggc ttccagccgc
     4561 agatgccggc aggcgtgcat ctgtcgaact ggtacgacca gagcgaactg gtgattgcct
     4621 cggcgtccag cgtgcgcgac gcgatcatga tcggcgtggt gctggccgcg ttgacgctgt
     4681 tcgcttttct gcgcaactgg aaaatcaccg cgatcgccgt cgcgctggtg ccggtcgtga
     4741 tggccgcgac gatcctgctg ctcgacgtgt tcggcatggg cttcaacatc atgacgctcg
     4801 gcggcatggc ggccgcggtc ggcctcgtga ttgacgatgc gatcgtgatg atcgaacaca
     4861 tcgtgcgacg catgcgggaa ggcggcgcac acgcgtttca cggccgcgtg atggccgccg
     4921 cgctcgaatt cacgcggccg ctcgcggggt cgtcggcggc gacgctgatc atcttcgtgc
     4981 cgctggcgtt tctgtcgggc gtgacgggcg cgtttttcaa ggcgctgtcg gtcaccatgg
     5041 cgagcgcgtt gttcatttcg tttctcgtga cgtggctcgc ggtgccgatt ctctgcgacc
     5101 gctggctgaa accgaaagac gccgaggaac acaaggacac ccgcttcgcg gcgtggatga
     5161 ataagcgcta cgcgtatctc gtcgagcgtg tgacggcgcg gcccattctt gtgctgctcg
     5221 gcctcgtgcc gctgatcctg gtggcggcgt tcgccttcac acgggtgggc agcggcttca
     5281 tgccgagcat ggacgaaggc ggtttcgtgc tggactacca cacggagccc ggcacctccg
     5341 tgactgaaac ggaccggctg atgaggcaga tcgagcgcat cattcgcgcg aatccgaacg
     5401 tcgcgaccta ttcgcgccgt accggcgcag gtctcggcgg cgacctcaac gagcccaaca
     5461 agggcgactt cttcatccgg ctgaagtcga gcggccgcga gccgatcgaa acggtgatgg
     5521 aagaaatccg ctcgcagatc gagacgcagg taccgggcgt gagcgtcgaa ctcgcccagt
     5581 tgatggagga tctgatcggc gacctcaccg cggtgccgca accggtgcag atcaagatct
     5641 attccgacga cccgaacacg ctcgacacca ccgcgcgcaa agtcgccgcg cggatcggca
     5701 agattcaggg cgtggtggac gtcaacgacg gcatcaaccc cgccggcgac gcactcgaac
     5761 tgcacatcca ccccgaagcc gcggccgccg aaggcatgga tccgcagtcg attgcgcaag
     5821 ccgtcaccga catggtggaa ggcagcgtgg cgacgcagtt ccagcgcggc ccgaaaacgg
     5881 tgggcgtgcg cgtgcgggtc gcgggcgcgg cgaaactgac cgatacgcaa ctggggcagt
     5941 tgcagattcg cgcaccggac gggcatctct tcgcactcaa ccgtgtcgcc gaccaggtga
     6001 cggtgaccgg ccagcccgaa atcagccgtg acaatctcaa gcgaatggtg gcggtgacgg
     6061 cacgcatcga cggccgcgat ctgggatcga cgattgccga tgtgcagaag accatgagcg
     6121 acaggagcct gttgcctgcc ggcgtgtatt acgaattggg cggcttgtat cagcagcagc
     6181 agattgcgtt caagggctta ttgaccgtgt tcggtgccgc gattgcgctc gtgttcgggc
     6241 tgctgctgtt tctctacgag cggtttcggg tggctctcgc cgtaatggcg atgccgttgc
     6301 tcgcggccgg cgcggtattc atcggcttgt ggatcaccgg gatcgagttg aacatctccg
     6361 cgatgatggg gatgacgatg atcatcggca tcgtgaccga ggtggcgatt ttttacgtgt
     6421 ccgagttgca gggactcatc acggatgagg gtgtgccgtt tgcagaggct ttgctcgcgg
     6481 ctggacgcaa tcgcctgcgg ccgatcgcca tgacgacgat tgccgcgatt ctggctttgc
     6541 tgccgttggc cttcgcgctt gggcaagggt cggcgatgca gcagccgctt gctgtcgcca
     6601 tcatttccgg gctgattgtg cagttgccgt tagtgctctt actgttgccc gtgctgctca
     6661 aactgctgat gaggcgagcc gtttaaggcg tcaaccgata ccgtcacacc gcctggcggt
     6721 atcggatcgg gttgtccggg gatgaaatcc cgataccgtt agggctaccg acattccaaa
     6781 cctgaccctc acgcgaggcg cccaccatcg cgtcccagcg ccttgctggc ggccatcgaa
     6841 acaacgcgag tgcgcttgac agcggcgctc cggctgcctg ataccgaacc gcgccacctc
     6901 gcccgcgtgt gctgaacggc agacgccggt gccgaggacg aaacagcatc cggtcgatag
     6961 gcgtgaatcg aggcatcgga cgatcccgac atctgcaact gcgcgggtcg aaccttgaat
     7021 aaatccgcct gaaaattgcc gccgccttcg tggacggacg gcaggtagga agctggcaac
     7081 gcatccctca agcttgccgc cgccgcatcg acgtcggctt gcagttcact cgcatgctgg
     7141 cgcacggtac ggtcgaccgt gtcctgcaca tgggtcactg cccgctcgat atcgtccttc
     7201 accttgcgca gttcgtcgat ccggatctgc tgatctactt ccgcccgcac gctgttcaag
     7261 taacgttgcg cgtgccccag taaggtgccg gcggtacgcg ccacacgcgg aagtcgttcc
     7321 ggaccgagca cgaccagggc aaccgtgccg atgacggcca gtttggaaat gtcgaagtca
     7381 aacatggcaa ccgtccctcc agattgttgc gaattcaagc acttgcacgg gtcattgtcg
     7441 cgaaacggcg ggcggatcct gcacgtcgat gtgcgcggcc accggcgctg ccttcgccgc
     7501 agcctctgca gtaaccggtt cgttttcctg catgccttcc ttgaaaccct tgatcgctcc
     7561 gccgaggtca gagccgagat tgcggatctt cttcgttccg aaaaccagcg caacgattac
     7621 cagaacgatc agccagtgcg taatgctaag gctacccatg
//
LOCUS       DATASH010000021         6443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5343763,
            whole genome shotgun sequence.
ACCESSION   DATASH010000021 DATASH010000000
VERSION     DATASH010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6443
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5343763"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1068)
                     /locus_tag="VHV99_00600"
     CDS             complement(<1..1068)
                     /locus_tag="VHV99_00600"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX3633996.1"
                     /translation="MRRYWQKKPLLIRQAIPDIRAPLSRDELFELADSEDAESRLITH
                     FRKSWAMEHGPFAPDELPSVKKRDWTLLVQGVNLHDDRARALLDRFRFIPDARLDDLM
                     ISYATDGGGVGAHFDSYDVFLLQVHGKRRWRFGAQRDLSLKEGLPLKVLQYFEADEEW
                     VLEPGDMLYLPPHIAHDGVAEGECMTASIGFRAPSASELSGQFLYHLAERLDEAHSAG
                     AKGSKGGAQRYSDPAQPAVSHPAQLPQLMIDRVGAILANIKWDEKDVASFLGTYLSEP
                     KPSVVFDPPRRPLNENRFIERVKQSGLNLDKKTVLLYDKHAYFMNGEQDLMSGKLKKW
                     LPELADKRYLEGKRFVTLSGDS"
     gene            1201..1740
                     /locus_tag="VHV99_00605"
     CDS             1201..1740
                     /locus_tag="VHV99_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006498638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX3633997.1"
                     /translation="MKIAKDTVVSVAYKLSDAHGNLIEESDEPMVYLHGGYDGTFPKI
                     EEALDGQEAGYQTQLQLEPQDAFGEYDPDLVKVEARGRFPEPLEVGMQFEGTPEDGED
                     ELDSLIYTVTDVAEDKVVLDGNHPLAGMALRFALNVQEVREATEDEKEHQHAHGLDGL
                     EVLDEDDEEPGKDVGPTLH"
     gene            complement(1835..3202)
                     /locus_tag="VHV99_00610"
     CDS             complement(1835..3202)
                     /locus_tag="VHV99_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008341849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3633998.1"
                     /translation="MVRSRVFVISLSALFLLAGGWQYARAEAPPLAPSTPLTFAIASN
                     VLDKPSGETALQHLLDAIGQERNTSFIVYDGNLKGETEPCRDSLYDTRQRIFDSSRKP
                     VVLLPGGNDWASCALAQAGSYDPVERLDFIRQLFFGDSGALGQTSMNVIRESDVARFR
                     PFRENVRWQANGVSFIGLNAPAPNNHYLTAGGRNGEFEDRSVANAFWLEHAVETARRS
                     EMRAVVVILQGDPDFARYERRDRFTWLHFSRGDASRDGFLELKRSLVKAAELFRGPVI
                     VIHESDTPIPRGFRIDQPLRNDKGTVVANLTRIAIGLKKPQSQWLEVESDLAWRPPFR
                     VRVRDVIVRPAAPSQASAPTVPASSSIPSSAASPSSSSASSPSIPGVETDTRGPSSFF
                     QQMQPVQPTQQMQHPAPARESDTGTPASSVPPILNLNSPANLPPILPVPSNPASSASG
                     SGTSQ"
     gene            complement(3264..5885)
                     /gene="mutS"
                     /locus_tag="VHV99_00615"
     CDS             complement(3264..5885)
                     /gene="mutS"
                     /locus_tag="VHV99_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008341848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEX3633999.1"
                     /translation="MMQQYLRIKQEHPGTLVFYRMGDFYELFFDDAVKASRLLDLTLT
                     QRGASGGNPIKMAGIPHHSCEQYLAKLVKLGESVAICEQIGDPATSKGPVERKVVRVV
                     TPGTLTDAALLSDKNDVYLLALCPAHNRRGVVTSVGLAWLNLASGALRLAEVAPDQLS
                     AALERIRPAETLIADSFSATNAPFEVPNIGATTRAPAWHFDIDSGTQRLCEQLDVAGL
                     DGFGAHSLTCACGAAGALLLYAAATQGQQLRHVRSLKVEYESEYIGLDPATRRNLELT
                     ETLRGTESPTLCSLLDTCCTTMGSRLLRHWLHHPPRDFTIAQGRQRAIGALLDAPATA
                     SLDGLRAALRQISDIERITGRLALLSARPRDLSSLRDTFAALPQLRERLANVKMNSAS
                     LERIDLSLEPPPECLALLSRAIAPEPAALIRDGGVIARGYDADLDELRDISENCDQFL
                     IDLETRERTRTGIANLRVEYNKVHGFYIEVTRGQTDKVPDDYRRRQTLKNAERYITPE
                     LKTFEDKALSAQDRALARERLLYDALLQTLLPHIGDCQRVAAALAELDLLGAFAERAL
                     ALDWIAPEFAGDAGIDIEQGRHPVVEAQVEQFIANDCCLNTERKLLLITGPNMGGKST
                     FMRQTALIALLAYVGSYVPAKRARFGPIDRIFTRIGAADDLAGGRSTFMVEMTEAAAI
                     LNDATPSSLVLMDEIGRGTSTFDGLALAWAIARHLLSHNGCHTLFATHYFELTQLPAE
                     FAQAANVHLSAVEHDHGIVFLHAVSEGPANQSYGLQVAQLAGVPPAVIKAARKHLAHL
                     EQQSIGQPSPQFDLFAPPVVEIADSHAHEEPAEAQTHHALDRLRAIDPNELRPRDALD
                     LLYELHELASNASDASR"
     gene            complement(6145..>6443)
                     /locus_tag="VHV99_00620"
     CDS             complement(6145..>6443)
                     /locus_tag="VHV99_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008341847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HEX3634000.1"
                     /translation="EGASDAVDTASTQLRSLLDDLDSAIRDAKDVDVDKLRKDLQTKL
                     KTARAQFDGSSSAIAGRLNDAAGYADEFVHEKPWHTLGAVAGLALLLGFLAGRS"
BASE COUNT         1250 a         1874 c         2070 g         1249 t
ORIGIN      
        1 tgaatcgcct gagagtgtta caaagcgttt cccctccaaa tagcgtttgt cggcgagttc
       61 agggagccat tttttgagct ttcccgacat taaatcctgc tcgccattca taaaataagc
      121 gtgcttgtca taaagcagca cggttttttt atcgagattc aaaccagact gtttcacgcg
      181 ctcgataaac cggttctcat tcaaagggcg tcgcggcgga tcgaatacaa cactcggctt
      241 cggttcgctc aggtaggtgc cgagaaatga agcaacgtcc ttctcatccc atttaatatt
      301 cgcaaggatc gcgccaaccc ggtcgatcat gagttgtggc aactgcgccg ggtggcttac
      361 cgccggctgg gccgggtcgc tgtagcgttg agcaccgccc ttggagccct tcgcgccggc
      421 gctgtgcgct tcgtccagac gctccgcaag gtggtacagg aactggcctg acagttcgct
      481 tgcggatggc gccctgaagc cgatggaggc ggtcatgcac tcgccttcgg ccacgccgtc
      541 atgggcgatg tgaggcggca aataaagcat gtcgcccggt tccagcaccc attcttcatc
      601 cgcttcgaag tactgtaaaa ctttcaacgg caagccctct tttagcgaga gatcgcgctg
      661 cgcgccaaag cgccagcgcc gtttgccgtg aacctgcaag aggaagacgt cgtaggaatc
      721 gaagtgcgcg cccacgccgc cgccgtcggt ggcgtacgaa atcatgagat cgtcgaggcg
      781 ggcgtcgggg atgaaacgga agcggtccag cagcgcccgg gcacggtcgt cgtgcaggtt
      841 cacgccttgc acgagtaatg tccagtcacg ctttttcaca gatggcaatt catcgggcgc
      901 aaaaggcccg tgctccatcg cccaggactt gcgaaaatgc gtgatgaggc gcgattctgc
      961 gtcttcgcta tcggcgagtt cgaacagttc gtcgcgcgat agcggcgctc gaatgtcagg
     1021 aatcgcctga cggatcagga gcggcttttt ctgccagtac cggcgcatga attgcgcagg
     1081 cgtgaggttg ccgagcaggg ttgcaggttc gtcgggaagc ggcaccgcga agggacttga
     1141 gggaacgtta ggggaccgcg ctggcatcgt ataattgagg cttgtatctt ggagaatcca
     1201 atgaaaatcg caaaagacac cgtcgtatca gtagcctata agctctcgga cgctcatggc
     1261 aatctgatcg aggaaagcga cgagccgatg gtctatctgc acggcggcta tgatggcacg
     1321 ttccccaaga tcgaggaagc actcgacggt caggaagccg gctatcagac ccagttgcag
     1381 ctcgaaccgc aggacgcgtt cggtgagtac gatcccgatc tcgtgaaagt cgaggcgcgc
     1441 ggccggtttc cggaaccgct cgaagtcggc atgcagtttg aaggcacccc ggaagacggc
     1501 gaggacgaac tcgattcgct gatctacacg gtcaccgatg tggcggaaga taaagtcgtg
     1561 ctcgacggca atcatccgct cgccggtatg gcgctgcgtt tcgcgctgaa cgtgcaggaa
     1621 gtccgcgaag ccaccgagga cgaaaaggaa catcagcacg cgcatggcct cgacggtctt
     1681 gaagtcctcg acgaggacga cgaggagcca ggcaaggacg ttggcccgac cttgcactga
     1741 ggcgtttata cgggttgttc gaagcgatgt gaaacaaggc gcaggcaggt tgctgcgcct
     1801 ttgttttatc tcgcgtaacg atccgtgaaa gggactattg actggtacct gagcccgatg
     1861 cgctgcttgc cggatttgat ggcaccggca ggatcggtgg caggttcgcg ggcgagttca
     1921 agttaagtat gggcggcacc gaagacgcgg gtgtgcccgt gtcggattca cgtgccggtg
     1981 cgggatgttg catttgctgc gtgggctgca cgggctgcat ctgctggaaa aacgatgaag
     2041 gcccgcgggt gtcggtttca acgccgggta ttgaaggaga tgaggcggaa gaagacgaag
     2101 gcgaagccgc cgacgagggt atcgacgaag acgcgggcac ggtcggcgcc gacgcctggc
     2161 tgggtgctgc cggtcgcacg atcacgtcgc gcacgcgcac ccggaagggc ggccgccatg
     2221 cgaggtcgga ctccacttcc agccattgcg attgtggttt cttcaaaccg atcgcgatgc
     2281 gcgtcagatt agccaccacc gtgcctttat cgttgcgcag tggctggtcg atacgaaagc
     2341 ccctgggtat cggcgtatcc gattcgtgga tcacaatcac gggtccgcga aacaattcag
     2401 cggctttgac aaggctgcgc ttgagttcga gaaaaccatc gcgcgacgca tcgccgcgag
     2461 aaaagtgcag ccatgtgaaa cgatcgcgac gctcatagcg ggcgaagtca gggtccccct
     2521 gaaggatcac gacgaccgcg cgcatctccg agcgtcgtgc agtttccacc gcatgctcca
     2581 gccagaatgc attggcgacc gagcggtcct cgaattcgcc gttgcgcccg cccgcggtca
     2641 gatagtgatt gttcggcgcg ggtgcgttca ggccaatgaa cgagacaccg ttggcttgcc
     2701 atcggacgtt ttcgcggaac ggccgaaagc gcgcgacatc gctttcgcgg atcacgttca
     2761 tggacgtctg gccgagcgca cccgagtcgc cgaagaacag ttgacggatg aaatccagcc
     2821 gttcgactgg atcgtaactg cctgcttgcg cgagagcgca agacgcccag tcgttgccgc
     2881 cgggtagcag gacgaccggt tttcgggagc tgtcgaagat tcgctgacgg gtgtcgtaga
     2941 gtgaatcccg gcacggttcg gtttcgcctt tgaggttgcc gtcgtagaca atgaacgacg
     3001 tgttccgttc ttgtccgatg gcatcgagca gatgctggag cgcagtttcg ccgctgggct
     3061 tgtcgagtac attcgaagcg atcgcgaatg tgagcggcgt agagggtgcg agcggcggcg
     3121 cctcggctcg cgcgtactgc catcctccgg ccagcaggaa gagtgcgctt aagctaataa
     3181 caaagacgcg gctgcgcacc atgcgccgcg cgcccggcca gtcggtggca gagtctgcct
     3241 ttacgccaac actaacgcca acgctaacgc gacgcatccg aagcgttcga cgccagctca
     3301 tgtagttcgt agagcagatc gagtgcgtcg cgcgggcgca attcgttcgg gtcgattgcg
     3361 cgcagccggt caagtgcgtg gtgcgtctgc gcttcggctg gttcttcgtg tgcgtgggag
     3421 tcagcgattt caacaacagg gggtgcgaac agatcgaatt gcggcgacgg ctggccgatc
     3481 gactgctgct ccagatgggc caggtgtttt cttgcggcct tgatgacagc cggtggtacg
     3541 ccagccagtt gtgcgacctg caagccgtag ctctggttcg ccgggccctc gctcactgcg
     3601 tgcaggaaca cgatgccgtg gtcatgttcg accgccgata aatgcacgtt tgctgcctga
     3661 gcgaactcgg ccggcaactg tgtcagttcg aaataatgcg tggcgaacaa cgtatggcag
     3721 ccgttatgcg atagcagatg acgcgcgatg gcccacgcga gcgcgaggcc gtcgaaggtg
     3781 gatgtaccgc ggccgatctc gtccatcagc acgagactcg acggtgtggc gtcgttgaga
     3841 atagctgccg cttccgtcat ttcgaccatg aaggtggagc ggccgccagc gaggtcgtcg
     3901 gcggcgccaa tgcgcgtgaa gatccggtcg attggtccaa agcgcgcgcg tttcgcgggc
     3961 acatagctgc ccacataagc gagtagcgcg atcaatgcgg tctgtcgcat gaacgtcgac
     4021 ttaccgccca tgtttggacc ggtgatcagc agcagcttgc gctcggtatt gaggcagcaa
     4081 tcgttcgcga tgaactgctc gacctgcgct tccaccaccg ggtgccgccc ttgctcgatg
     4141 tcgatacctg cgtcgcccgc gaattccggt gcgatccagt cgagcgcgag cgcccgttct
     4201 gcgaacgcac caagcagatc gagttcagcg agcgccgccg ctacgcgctg gcagtcgcct
     4261 atgtgcggca acagcgtttg cagcaacgcg tcgtagagca aacgctcgcg tgccaatgca
     4321 cggtcctgtg cggacagcgc cttgtcttcg aaggtcttca actccggcgt gatgtaacgc
     4381 tcggcgtttt tcagtgtctg gcgacggcgg tagtcatccg gaaccttgtc cgtctggccg
     4441 cgcgtgactt cgatataaaa gccatgcacc ttgttgtatt cgacccgcag gttcgcaatg
     4501 cccgttcgcg tgcgttcacg cgtttccagg tcgatcagga actggtcgca gttctcggag
     4561 atgtcgcgaa gctcatcgag gtcagcgtcg taaccgcgcg caatcacgcc cccgtcgcgg
     4621 atcagcgccg cgggttcggg tgcgattgcg cggctcagca acgcaaggca ctcaggcggc
     4681 ggctcaagcg ataggtcgat acgttccagc gatgccgaat tcatcttcac gttagccagc
     4741 cgttcgcgca gttgagggag cgccgcgaag gtatcgcgca ggctggagag atcgcgtggc
     4801 cgggctgaca gcaacgcgag ccgccccgta atccgttcga tgtcggaaat ttgccgcagc
     4861 gccgcgcgca aaccatcgag gctcgcggtt gcgggtgcgt cgagcaatgc gccaattgca
     4921 cgctgccggc cttgcgcaat ggtgaagtcg cggggcggat gatgcagcca gtggcgcagc
     4981 agacggctgc ccatggtcgt gcagcatgtg tccagcagcg agcacaacgt gggggattcc
     5041 gttccgcgca aggtctcggt cagttcgaga ttgcgccgcg tagccgggtc gagaccgatg
     5101 tactcggatt cgtactccac cttcaggctg cgcacgtgcc gcagttgctg cccttgagtt
     5161 gcggccgcat acagcagcag cgcgcccgcg gcgccacacg cgcaggtcag cgaatgtgca
     5221 ccgaaaccgt cgaggcccgc gacatcgagt tgctcgcaca gacgctgcgt gcctgaatcg
     5281 atatcgaaat gccatgcagg cgcgcgggtt gtcgcgccga tgttcggcac ttcgaacggc
     5341 gcattggtcg cggagaaaga gtcggcgatc agcgtttcgg ccggacgaat gcgctcgagc
     5401 gccgcactca actgatcggg cgcgacttca gcgagacgca atgcgccgct cgccagattc
     5461 aaccatgcga gaccgacgct cgtgaccacc ccgcggcggt tgtgcgccgg gcagagagcg
     5521 agcaggtaga cgtcgttttt atcggagaga agcgccgcgt cggtcagcgt gccgggtgtg
     5581 accactcgca cgaccttgcg ctcgaccggt cccttcgatg tcgccggatc gccgatctgc
     5641 tcgcaaatag cgaccgattc cccgagcttc acgagcttcg ccaggtactg ctcgcacgaa
     5701 tgatggggga tgccggccat cttgatcgga ttgccacccg acgctccgcg ttgcgtcagc
     5761 gtcaggtcga gcaggcgcga ggctttgacg gcgtcgtcga agaacagctc gtagaagtcg
     5821 cccattcgat aaaacacgag cgtgccaggg tgctcctgtt tgatgcggag atactgctgc
     5881 atcatgggag tgtggtgcga cagatcggcg ccgccgggtt cctgcgcggc gcggcccaag
     5941 tcttgagcgg tagacgaaac ggagatatgc gtacccatct tgcgtgcgtg aatttgggaa
     6001 agtcctaagg gcacgagtgt accgcgtcgg ctcgtcgcgg gaacgctggc cgaacggcta
     6061 acgcggggcg gggcgcacac tcacaaaagc aaaacgccgg acgtgccggc gttttgcttt
     6121 acctcgaaca taacgcaaga ccaatcagga tcgtcccgcc aggaacccca gcagcagcgc
     6181 gagtcccgcc accgcgccga gcgtgtgcca gggcttctcg tgtacaaatt cgtcggcgta
     6241 accggcggcg tcgttcaggc gtccggcaat tgccgaggac gaaccgtcga actgtgcacg
     6301 tgccgtcttc agcttggttt gcaagtcctt gcgcagcttg tcgacgtcga cgtctttcgc
     6361 atcgcggatc gcactatcca gatcgtcgag caggctgcgc aactgcgtgc tggcggtatc
     6421 gaccgcgtcg cttgcgccct cta
//
LOCUS       DATASH010000022        20325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2970060,
            whole genome shotgun sequence.
ACCESSION   DATASH010000022 DATASH010000000
VERSION     DATASH010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 20325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20325
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2970060"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            506..1168
                     /gene="parA"
                     /locus_tag="VHV99_00625"
     CDS             506..1168
                     /gene="parA"
                     /locus_tag="VHV99_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014900706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family partition ATPase"
                     /protein_id="HEX3634001.1"
                     /translation="MAAEIIAVTQQKGGVGKSTIAMHLGAAFHEKGKRVLVVDADGQN
                     TLVHWASASSDGETGIPFPVVNLSEAGSQIHREIKKFVADYDIIVVDCPPSITEKVSG
                     VVLLAASVAIMPTSSSPADYWSSVGLVKLVQQAQVMNEDLRAVFLLNKTEEKRMLTRE
                     LKRALEELGFPLLKTQIPTREAYKQAMALGQTVLQMNDRGAKLAAIEVRACANEIAAL
                     LP"
     gene            1193..2248
                     /locus_tag="VHV99_00630"
     CDS             1193..2248
                     /locus_tag="VHV99_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX3634002.1"
                     /translation="MKPSQFAKGFQARPDTTSSEKRTALDRLNAIDGLVKNGTQAGDV
                     PSRSLQPVMSQDIAEVETADNANESAAYRAWRIEHAYRPGQVIELALKTIKSSPFNPR
                     HFYLKSSIAELAVNLAKQGQQQAIHVIPDYDNPGTYFVSDGGRRVRALKEANKETVKA
                     IVIDLPIGLQSYKLGYDLNVQRDSQTVFDNAVVWKRFLDDRHFQSQKELAEHLGLDES
                     TVAVALSIAKLPEAVMHEMVARPDRFGSNMAYQVGRYHTARGADATLRLINKILSDDL
                     STRQVADIVKGRASAQESTKPAGRQRYAQRLEIKLGGIAVGDLKSYGDDRIELRLKGL
                     TREKRDDILRQIEKMLK"
     gene            complement(2316..3665)
                     /locus_tag="VHV99_00635"
     CDS             complement(2316..3665)
                     /locus_tag="VHV99_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEX3634003.1"
                     /translation="MATKRAKKTDVVSPSSAELRKAVEAIAIQPKSGKITLLTRKLFN
                     VLLAVAQQADESGDTYRALLSDIVANSAFDSNDTALVKEHLRRMVSVQVEWSTGTSSQ
                     KPGRKWGISTLIADAEILEDPTTRRVWVEFSFAPKIKKKLLDPVQYARLSLQFQSQLR
                     SSAGLALYEICVRYLTNPSHLTMRETWEWWRPILSGTPDTEAGDEAKREYKYFKRDYL
                     RPAIAEVNAVTNIFVELIEHREGRRVAEIQFRVTERKQPMLALDEHPNVFDSTLVDRM
                     VKIGIPLKEAQTLYADSEENRIRAALQMTEQRMRSTSLPAVRSAPALFKDALKKGYAP
                     PVEALPSGSGGKAAVAAPADDLKARLLGEYSAYRRKEARELYDEQGESEREIARKSFE
                     EDQLPDLGTHMRDDWRRRGLDSKIVETAFFDWLAHKTWGEPTDGDLLAFTLSQSRAA"
     gene            5579..6784
                     /locus_tag="VHV99_00640"
     CDS             5579..6784
                     /locus_tag="VHV99_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEX3634004.1"
                     /translation="MNLDQERQAIRDELESLRAGGARRQELSLHACKRLFFDLGIRPS
                     MAAVRDLTQTGSASDIPKDIDHFWERLRTVSRVKVGAGAIPKALEDRAGELLSALFEE
                     AIVHARSSLDGEREEIRAQVAAADQRAREAEIRHSMSEEAVKRSELKAEAAWERIRAL
                     ETELSSTTTHGTAHQEGLQATARRLDSENETLRQRLDTEQATNAALRDRIDALHVELR
                     QSTEHYAQQIKDAVAEAERRVKPMLVELDSLRSMAATYQSGVRDASRKEFEFIQQIAA
                     AKARGDRLDAQLREQSDEVDVLAKEVAALRGQQGIAPAIASLLCSLVDAGRLTTDELT
                     VIGTAADGHVTLPLRCPKCEEGEPELSQVDHHFELQCPECDHSSGPGESRLAAVSRFL
                     SSASIAASA"
     gene            complement(6871..7938)
                     /locus_tag="VHV99_00645"
     CDS             complement(6871..7938)
                     /locus_tag="VHV99_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX3634005.1"
                     /translation="MSPPHLSDPVPRPAPSTDLFDQEREDWRRDPQIAFDAWLAKQHF
                     RRSSAEVYQAQWGLFLDWLAVRQKSLVTVDTQTIAEFVGGLAIRKPQRVRYLRLIERV
                     LDHVREIESASTNPARFIAQDGEAAWRNARDNEPTGFLNHTERAALIAQLFSPLPDLS
                     MAQRWRERRDRALIAVFLGGGLKTGEAAAITVSCVNTGSPWVTIESANPMLTRRTRLA
                     PFAAAILDAWLAERRLSELAGNLVFPASPSGRPMHKATMLRAVDALIDAAGIAASRTS
                     RASPQTLRNTFAADLFESGVEAELVGQWLGFVQAVSANRLYRAWQKWMDRQDSPVIDE
                     PDPAAPPLPTPRQDRRFTRKG"
     gene            complement(8002..8400)
                     /locus_tag="VHV99_00650"
     CDS             complement(8002..8400)
                     /locus_tag="VHV99_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2471 family protein"
                     /protein_id="HEX3634006.1"
                     /translation="MNDLTTEQAEHALAALRYQTAARDLEHIVRNIAARYIVQQVPLS
                     WRLLHAIEAEALADLGFASRHDAIMLGLFQRPSDLPYPETDEAVDFGTSTALPAVFAF
                     AVSAYEEAARRAAQPSSKGAALKHARAWGD"
     gene            complement(8516..9142)
                     /locus_tag="VHV99_00655"
     CDS             complement(8516..9142)
                     /locus_tag="VHV99_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007732843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEX3634007.1"
                     /translation="MASSGIRRAAANAAPTSGTGTVPATAAPPRVGEQIQRLRAERRM
                     TLDDLSRAAGVSKSMLSEIERDKANPTIAVAWRLTNALGVSLDSLFAPQKAPEAIAVA
                     GPHDIPTLSGHEAKYQLRVWGPIELAGKFEWYELTLQAGGALVSNAHEPGTREHLTVL
                     QGSIEIEAAGTTRRLKAADTARYVADEPHAIRNAGKGEAKALLVVIHG"
     gene            9312..10526
                     /locus_tag="VHV99_00660"
     CDS             9312..10526
                     /locus_tag="VHV99_00660"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEX3634008.1"
                     /translation="MRDLYLAHLRGTLEQIRADGFYKNERVIASPQSADVRLANGTEV
                     LNFCANNYLGLANDERLIEAAKQGLDHDGFGMASVRFICGTQTVHKDLEKALAAFLQT
                     DDCILYSSCFDANGGLFETLLDENDAIISDELNHASIIDGVRLSKAKRFRYKNNDLAD
                     LEAKLKEADAAGARFKLIATDGVFSMDGIIANLAGICDLADRYGALVMVDDSHAVGFV
                     GEHGRGTPEHCGVLSRVDIMTGTLGKALGGASGGYVAARQEVVDLLRQRSRPYLFSNT
                     LTPSIAAASLKVLDLLASDEGAQLRARVRENGAHFRSAMSALGFTLVPGEHPIIPVML
                     GDAQLASNMADALLKEGVYVIGFSFPVVPKGRARIRTQMSAAHTPEQIDRAVDAFARV
                     GRQLGVIERRRT"
     gene            10523..11554
                     /gene="tdh"
                     /locus_tag="VHV99_00665"
     CDS             10523..11554
                     /gene="tdh"
                     /locus_tag="VHV99_00665"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEX3634009.1"
                     /translation="MKALAKLERAPGLTLTDVKKPEVGHNDVMIRITRTAICGTDIHI
                     WKWDDWAQKTIPVPMHVGHEYVGEIVEMGQEVRGFAIGDRVSGEGHITCGFCRNCRAG
                     RRHLCRNTVGVGVNREGAFAEYLVIPAFNAFKIPPEISDDLAAIFDPFGNATHTALSF
                     NLVGEDVLITGAGPIGIMAVAIAKHVGARNVVITDVNDYRLELARKMGATRAVNVARE
                     SLRDVMADLHMAEGFDVGLEMSGVPSAFTSMLEAMNHGGKIALLGIPPAQTAIDWTQV
                     IFKGLEIKGIYGREMFETWYKMVAMLQSGLDLSPILTHHFKVDDYQEAFATMLSGESG
                     KVILDWTAA"
     gene            complement(11647..12297)
                     /locus_tag="VHV99_00670"
     CDS             complement(11647..12297)
                     /locus_tag="VHV99_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX3634010.1"
                     /translation="MSRSTFRATLVRALLSATLLTAMACTPLQVVTHTVSQNEARVPV
                     GRYDLDPDHCSITFDIDHFKYSRFTMRFDRKHGQLDWNEGGLDKSSATITIDAASIDT
                     NVPLLDKMVKSASMLDVSRYPEVRFTSTRFERTGESRGMLTGDLTIHGVTQPVTLDVT
                     FNGFAPDPLTKKDTLGFSADGHFSRAKFGLATWYPAVGDDIHVRIQAEFVKTPAGG"
     gene            12406..13014
                     /locus_tag="VHV99_00675"
                     /pseudo
     CDS             12406..13014
                     /locus_tag="VHV99_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007732840.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            13205..13684
                     /locus_tag="VHV99_00680"
     CDS             13205..13684
                     /locus_tag="VHV99_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX3634011.1"
                     /translation="MADPIETRPPLPPFTRETAIQKVRAAEDGWNTRDPERVSLAYTP
                     DSVWRNRAEFTHGRAEIVGLLRRKWARELDYRLIKELWAFTDNRIAVRFAYEWHDDSN
                     NWFRSYGNENWEFDEHGLMARRHASINDLPIREADRLYHWPLGRRPDDHPGLSDLGL"
     gene            13914..14432
                     /locus_tag="VHV99_00685"
     CDS             13914..14432
                     /locus_tag="VHV99_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634012.1"
                     /translation="MHFRQIKKQIRHTVVALVASLAAASAAHAGDWCEGGVWVDAMLG
                     SYHINPDPNTHFEQFNPGLGVECWLNNQWAVTAGGFRNSLRRPSYYGGGVWAPEFAHW
                     GIVRIAAIGGIISGYNYGNWGLGRNHTIGPVVAPIVMVEYKRVGANFIVIPPIPSDNL
                     PFTIGFQLKVKF"
     gene            14993..16723
                     /locus_tag="VHV99_00690"
     CDS             14993..16723
                     /locus_tag="VHV99_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEX3634013.1"
                     /translation="MTNHSSGSASGSLDRVSAWSLIRPYWVSEERGTAWGLLIAIIAM
                     DLLLVGINARLNTWNRDFYDALESRNVRDFPQLMLMFAGLAFASITISVYNRYLRQML
                     GFRWRQWLTTRYLKEWLGDGTFYRIERDRLVDNPDQRIAVDLDLLATATLSLTLDLLS
                     TIETLVWFSIVLWSTAGALVIMMGGTPVQIQGYMFWAAVAYAIVGSLLTNKVGHPLVS
                     INYQLQRVEADFRFGLIRLRENAEQIALYDGMRTEASNAENLFGRIRENWWRLMRYTK
                     RYSFVVNFYGQIAEIFPIVVASPRYFSGALSFGTLMQIADAFGSVSESLSWFINNYDT
                     LVEWRATVNRLGEFKRVVRQPHLKESVSPATEHGGIKLHYVDENQLFTHDLMLALPNG
                     ETLASVPDIVVKPGSRWLVRGPSGSGKSTLLRALAGLWPFGDGSIDAPVDARMMFIPQ
                     QSYLPVGTLKAALAYPTPAESFSDDDCREALRECRLEEYVGRLQESDHWWRILSPGEQ
                     QRLAVARVLLHKPDYVFLDEATSALDTENEAHLYRLLTERLPKAAMVSVAQHESLVQF
                     GQETLDIRRP"
     gene            16821..16980
                     /locus_tag="VHV99_00695"
                     /pseudo
     CDS             16821..16980
                     /locus_tag="VHV99_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006481520.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            17096..17962
                     /locus_tag="VHV99_00700"
     CDS             17096..17962
                     /locus_tag="VHV99_00700"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3634014.1"
                     /translation="MVGSERRVSVVTGATGGIGRWIALGLAQADHHVVLVCRDAVKGR
                     ALADWISQQAPAASTELRLADLSSLCAVQRLGQEIAAVHPRLSVLINNAGMFTARRST
                     TAEGHDIVLALNHLAPLVLTDALEPALRAGSPARIVNVGSSTSERARIDPDDLELVRH
                     WGMVRAYGRSKLALMMSTFARAQRLRGSGVVANVVHPGTVATGLVREGGAIGAAWRLM
                     GPFLRTGQQGADTPLHVALAPAWAAATGLYVKDRAAVRPNRRALDPALVEKVEAASRE
                     LAGRTTVVDIPL"
     gene            complement(17992..19080)
                     /locus_tag="VHV99_00705"
     CDS             complement(17992..19080)
                     /locus_tag="VHV99_00705"
                     /inference="COORDINATES: protein motif:HMM:NF005495.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3634015.1"
                     /translation="MPSDPDFLMQSSHHASPNQRTEARVIVITGASSGIGRGTANFFA
                     RYGWRVGLIARGRAGLEAVFDDVHRHGAMAAMEQADVTDLDALEAAAARIEAVLGPID
                     AWINCAGNGTYGRFLDTPAEQFRRVTDVTYMGTVNGTRVALRRFIPRDRGSIVNVCSA
                     VAFHGMPLLSAYSGAKHAVRGFGQSIRAELSQDGSHVRLTSVFPPAVNTPFFDHAISH
                     MGQLGRPMSPVYQPEVVVEAIRLALISGRAEMPVTSTAILFSLGVRLVPGLVAAAIRK
                     LSYNGQLTDEAALKERHAPTLFDASDRPSPVRGAFDAQARSGSVHIRILSMLARLRQH
                     ARWEPQAGRQASPDRPADETTVQEHALD"
BASE COUNT         4253 a         6165 c         5977 g         3930 t
ORIGIN      
        1 ccagctggag gcagcagcac ttgactcgac agacagtgtc agttcgtttc gtcattcccg
       61 acttgatagc tgcctttcct ggtgaggaaa cgtcaagccc gaagccgcga agcagtgcga
      121 cagagggcgc aggcttgagg ctcgacttgc cccggagaat cattcccata gtcgggactg
      181 gacgatacgc tgacattgtc tgaccggcag agcacccgct aatacacccc agctattcca
      241 atcgcttagc ctcatacacc ttagcttgca tgaccacctc actccttctc aagaaaccca
      301 tcatcgccaa gattctccgg acgccaccaa acctgtcact gcctatagcc acctcgcagt
      361 cacacgccga accgccaccg cccacatcct ttatgcgcat aaacctaacc caaacggcta
      421 attcttctcg cgtttacaca aaaacaattg aaatgcgaat tcatccaggt aaaatccatc
      481 tgcgtctaaa aagaggaggt gaagattggc agcagaaatc atcgcggtaa ctcagcagaa
      541 gggtggggtc gggaagagca caatcgcgat gcacctcggc gctgcattcc atgagaaagg
      601 caagcgcgtc cttgtcgtag acgcagatgg tcaaaacact ctggtccact gggccagcgc
      661 atcttcagac ggcgaaaccg gcatcccctt tccggtcgtc aatctctccg aagccggtag
      721 ccagatccat cgcgagatca aaaagttcgt ggcggactac gacatcatcg tcgtcgactg
      781 tccgccttcc atcaccgaga aagtgtccgg cgtcgtgttg ctcgcggcga gcgtcgcgat
      841 tatgccgacc tcgtcgtcac ccgccgatta ttggtcgagc gtcggactag tgaagctcgt
      901 gcagcaagct caagtgatga acgaggacct gcgcgcagtc tttctgctga ataagaccga
      961 agagaaacgg atgctgacgc gcgaactgaa gcgggcgttg gaagagctcg gtttcccact
     1021 gctcaaaacc cagattccga cgcgcgaagc ctataagcag gcaatggcgt taggacaaac
     1081 agtccttcag atgaacgatc gcggcgccaa gctcgccgcc atcgaagtac gggcatgcgc
     1141 caatgagatc gccgccctgc tcccctgaat ttatgcgcat aaaggaccct gaatgaaacc
     1201 ctcccaattc gccaaaggct ttcaagcacg tcctgatacg accagcagcg aaaagcgaac
     1261 cgctctcgat cgtctgaatg cgattgacgg tttggtcaag aatggcaccc aggccggcga
     1321 tgtaccgagc cgcagcctcc agccggttat gtcgcaggac atcgcggaag tcgaaaccgc
     1381 cgataacgcc aatgagtcgg cggcctaccg tgcgtggcga atcgagcacg cataccggcc
     1441 gggccaggtt atcgagctcg cgctcaagac gatcaagtcg agtccgttca atccacgcca
     1501 tttctatctg aagtcatcga ttgccgagct tgccgtcaat ctcgcgaagc aaggccagca
     1561 gcaagcgatt cacgtcattc ccgattacga caatcccggc acctacttcg tcagcgacgg
     1621 tggccggcgt gtgcgagcgc tgaaagaagc aaacaaggaa acggtcaagg cgatcgtcat
     1681 cgatctgccg atcggactcc aaagctataa gctcggctac gacctcaacg tccagcgcga
     1741 ttcgcagacg gtgttcgaca acgccgtcgt ctggaagcgc tttctcgacg atcgacattt
     1801 ccagagccag aaggagctcg ccgaacatct cgggctagac gagtcgaccg tcgcggtcgc
     1861 cttgtcgatt gcgaagcttc ccgaggcggt gatgcatgag atggtggcga gacccgaccg
     1921 cttcggctcg aacatggcct atcaggtggg ccgttatcac accgcccgcg gcgctgacgc
     1981 gacgctgcgc ctgatcaaca aaattctctc ggacgacctg agcacgcgac aggtcgcgga
     2041 cattgtgaag ggcagggcaa gtgcacagga aagcaccaag ccggcgggac ggcagcgcta
     2101 tgcgcagcgt ctggaaatca agctcggcgg tatcgcagtc ggcgatctga aatcgtacgg
     2161 ggacgatcga atcgaactgc gcctgaaggg actcacgcgc gaaaaacgtg acgacattct
     2221 tcgacagatt gaaaagatgc tgaagtaaca gagcggaaag cttcaggccg aaagagtcgg
     2281 cggcgcacga gagtggccgc cgtttttctt gacgattatg ccgcgcgcga ttggctcagc
     2341 gtaaaggcga gcaagtcgcc atcggtgggt tcgccccaag ttttgtgagc cagccaatcg
     2401 aagaacgccg tttcaacgat cttcgaatcc agcccacgac ggcgccaatc atctcgcatg
     2461 tgagtgccga gatccggcag ctgatcttcc tcaaacgact tccgcgcgat ctcccgctcg
     2521 gactcacctt gctcgtcata aagctcgcgc gcttccttgc ggcgatacgc cgagtactcg
     2581 ccgagcagac gtgctttcag atcgtccgca ggcgcggcca ctgcggcctt accaccgctg
     2641 ccggacggca gcgcttcgac cggcggcgca tagcctttct tcaacgcatc cttgaacagc
     2701 gcgggcgcac tgcgcacagc cggcaaagac gtgctgcgca tacgttgctc ggtcatttgc
     2761 agcgctgcgc gaatccggtt ttcctcacta tcggcgtaca gcgtttgcgc ttccttcagc
     2821 ggaatgccga tcttcaccat ccggtcgacc agtgtgctgt cgaacacgtt cgggtgttcg
     2881 tcgagtgcaa gcatgggttg cttgcgctcg gtcacgcgga actggatctc ggcgacacgc
     2941 cgcccttccc ggtgctcgat caactcgacg aagatgttgg tgacagcatt cacctcggcg
     3001 atagccggcc gcaggtaatc acgtttgaag tacttgtact cgcgtttcgc ctcgtcgccc
     3061 gcttccgtat ccggcgtgcc ggagaggata ggacgccacc attcccatgt ctcccgcatc
     3121 gtaagatggc tcgggttggt cagatagcga acgcaaatct cgtaaagtgc gagacccgcg
     3181 ctactgcgca actggctttg gaactgcaga ctcaggcgcg cgtactgaac cggatcaagc
     3241 agcttcttct tgatcttcgg cgcaaaggag aactccaccc agacgcggcg ggtagtgggg
     3301 tcttcgagaa tttccgcgtc ggcaatcagc gtggaaatac cccacttacg gcccggcttc
     3361 tgactggacg tgcccgtgct ccattcaacc tgcaccgaca ccatgcgccg cagatgctcc
     3421 ttgacgagcg ccgtgtcgtt cgaatcgaac gccgaattgg cgacgatgtc cgacagcaga
     3481 gcccgataag tgtcaccgga ctcgtccgct tgctgagcaa ccgcgagcag cacgttgaat
     3541 agcttgcggg tgaggagcgt gatcttgccg ctcttgggct gaatcgcaat cgcctcgacg
     3601 gccttgcgca attcggctga gctcgggctg actacatcgg tcttcttcgc gcgcttcgtg
     3661 gccatgcgtc tggtcgagga gggatttggc gagagcatac cggctgtggt gatacgcgtc
     3721 tatagcatgg gtctcaccgt tcccggtgaa gtgagtttac gacgcgtgac tcacctcgag
     3781 ccgctcccga aaaccctcac ctcaacagtt tcgcgccagg ttcggggcat ccttacaggt
     3841 cgcggcctcc cgaaatgcgg agcgttttcc gactcgcagt gagcgcgagc caagcgatcc
     3901 cgaatcttct cacctcaaaa aaatctcgtt aattttggcc gtgtgcatgt tgcaagccgg
     3961 aagcactcgc gctactcggt gggtgtgaac gccggtgatg tgaacgcgcc ttggcccatt
     4021 caacggatgg gggaggggag gaaagtgcgc acccatcaat tgcggccgta cgggccttcg
     4081 ccaggcagag acgcacgcag ccgcccggtt ccgtctcaag caaaggaggc taaacgcggc
     4141 agacagattg caggccggca cctcattcgt acgagagtga tccgaacgcg caacgttccc
     4201 ccgaaaaagc tcaccttaac gctagctgag cgcttggaac gacgcggcct tcagacctgg
     4261 aagcctgaaa aaacaccgag aaatactccc gaaaaacctc accaatgagc ccggaagcgg
     4321 tttgcgaggg cctcaaaacg gtcccccgaa aaacctcacc tttgcgctgc gcacaaaaat
     4381 aactcaacga tttcatcggg ttacaggaca acctcgagcc acgccgcgga gaagccctcc
     4441 cgtatatcct caccttcaac cggtattcaa cgaaatccag gcattttcgg gcgcaactcg
     4501 attggtcccg aaaaacctca cctcaagatt ccagcaagcc caaaacggga atagctctga
     4561 cagcgtccgt agggcttttc cggcctatcg aagcgattct ggtcccgtat ttcctcacct
     4621 tagcgattac aaacagccca aaagactccc gaaaatcctc acctttggcg gaaaacaggc
     4681 aatttgaaga gccgtcgatc gctccaccct aggtctcccg aatttgctca cctttcctcc
     4741 aaggcggccc aggagctctg caatgggaag aacagaatgc caggcgaaat ttcaactcag
     4801 ctcccgcaaa ccctcacctt aagcgcctgt ttgcccagaa ttgaggcaaa aatgacatct
     4861 agtccccgaa aagtctcacc ttcggtccgc gttttctcct gtcttgactc accaatgggg
     4921 tttacgcggc ccaaataagg ccaaaatttg cggttcactc cggtcccgaa atcactcacc
     4981 ttccgggcag tcgtgcgctg acccgacttt tgagggctgc cgtgcgatcc cggacgttca
     5041 ggtcccgcaa aatctcacct ttagccaaat cacacttttg gggcggctcc tgaacccgct
     5101 cacgttctgt cccggaccca ctcaccgagg ctcccgaacc tcatcactcg acagtcccgc
     5161 aagatctcac cacatctccc ggaaagcttc accttaccgg gctgaaacac cagccaggca
     5221 agggtttgcc gtggcgttaa cgtttttaac atgggttcaa aggtgtttac ttttattact
     5281 ctctagcatt accccgcgtg tccttcggac agcacctttc cagccacttc gcgcccatga
     5341 cacgttcgtg aaacagtgag cgatcagaac aaattcttca aaatcacttt atttacaaag
     5401 gcttattggc taatcttcgt tttgtttcac ggaaaaagcg cgcaggttcg aatccgatgt
     5461 cttactagtg gctctaaaac agcccaacta accaaagcgc acgttctcca cgcaaatatg
     5521 tagcggacaa tacgtaaatt gttatgattc atccaattcg agcagcatcg aggtttacat
     5581 gaatctggac caggaacggc aagccatccg cgacgagctt gaatcgttga gagccggcgg
     5641 agcacggcgg caggaattgt cactgcatgc ctgcaagcgg cttttttttg atttggggat
     5701 tcgcccatcc atggcggctg ttcgcgacct gacccaaaca ggcagcgcaa gcgacatccc
     5761 gaaggacatc gaccattttt gggaacgcct cagaactgtg tcgcgcgtca aagtcggtgc
     5821 cggcgctata cccaaggcac tcgaagatcg cgccggagag ttgttgagcg cactctttga
     5881 agaagccatc gtgcatgcac gctcaagctt ggacggcgaa cgcgaggaaa ttcgcgctca
     5941 agtcgccgcc gccgaccagc gcgcgcgaga agcggaaatt cgccacagca tgtcggaaga
     6001 agcggtcaag cgcagcgaac tgaaagctga agccgcgtgg gagcgcattc gcgcgctcga
     6061 gacggagcta tccagcacca ccacacacgg gaccgctcac caggaaggct tgcaggcaac
     6121 ggcccgccga ctcgacagcg aaaacgaaac gctgcgtcaa cgtctcgaca ctgaacaggc
     6181 gaccaacgca gcgcttcgcg accgaatcga tgcacttcat gttgaattgc gccaaagcac
     6241 cgaacactat gcacagcaga tcaaggacgc cgtggctgaa gccgagcggc gtgtgaagcc
     6301 gatgctggtc gaactcgatt cgttgcgcag catggcggcc acctatcagt cgggggtgcg
     6361 tgacgcaagc cgcaaggaat tcgagtttat tcagcagatc gccgcagcca aggcgcgcgg
     6421 tgaccggctc gatgcgcaac tgcgcgagca gtcggatgaa gtggatgttt tagcgaaaga
     6481 agtcgccgcg ctgcgtggcc aacaaggcat cgcccccgcg attgcaagcc tgctgtgctc
     6541 gttggtcgac gcgggcagac ttacaaccga tgagctgacc gtaatcggca ccgcggcgga
     6601 tggccatgtc acgctgccac tgcgttgccc aaaatgtgag gaaggtgagc cagagctatc
     6661 gcaagtggat caccacttcg aactgcagtg tccagaatgc gatcactcat caggccccgg
     6721 ggaatcaagg ctggcggcgg ttagccggtt tttatcaagt gcgtcgattg cagcgtcggc
     6781 atgaccgtcg acgcgacgca accctggagt aagcgacaaa ggtgaggaaa ttcgggacct
     6841 caagggtgag ggctttcagg ggtcgccgga tcaccctttc ctcgtaaaac gcctgtcctg
     6901 ccggggagta ggcaagggcg gagcggctgg atccggttcg tcgataaccg gcgaatcctg
     6961 gcggtccatc cacttctgcc acgcccgata caaacggttg gcggacactg cctgtacaaa
     7021 tcccagccat tgcccgacca gctctgcctc taccccgctt tcgaatagat ctgctgcaaa
     7081 ggtgttgcgc aacgtctgcg gactggcgcg tgaggtgcgc gaggcggcaa ttcccgccgc
     7141 gtcgatcaac gcgtcgacgg cgcgcagcat ggtggccttg tgcatggggc gcccggacgg
     7201 cgaagcggga aagaccagat tgccggccag ttcggaaagg cgccgttcgg cgagccaggc
     7261 gtcgagaatc gcagccgcga agggcgcgag ccgggtacgc cgcgtcagca tgggattcgc
     7321 ggactcgatc gtgacccacg gtgagcccgt attcacgcaa ctaaccgtaa tcgccgcagc
     7381 ttcgcccgtc ttgagtccgc cgcccagaaa cacagcgatc aaagcacggt cgcgccgctc
     7441 tctccagcgt tgcgccatgg acaagtccgg caacggagaa aacagttgcg caatgagcgc
     7501 ggctcgttcg gtatggttga gaaaaccggt cggctcgtta tcgcgtgcat ttcgccatgc
     7561 tgcttcgcca tcctgagcaa tgaagcgggc gggattggtc gacgctgatt caatttcgcg
     7621 cacatggtcc agcacgcgtt cgatgagccg caaatacctg acgcgttgcg gttttcggat
     7681 tgcaagcccg cccacgaatt cagcaatggt ttgcgtatcg accgtgacca gactcttttg
     7741 acgaacggca agccagtcca aaaaaagccc ccactgcgct tgatagactt cagcggaaga
     7801 gcgcctgaaa tgctgcttgg caagccatgc gtcgaacgcg atttgcggat cgcgacgcca
     7861 gtcctcgcgt tcctgatcga agagatcggt tgatggtgcg gggcgcggga ccgggtcgga
     7921 cagatgagga ggtgacatac gctttctttc cgttagttgc gcatattgta gatcgcaacc
     7981 ggtggaagta cagcgcctaa gttagtcgcc ccacgcccgc gcatgcttta gtgcagcgcc
     8041 tttggacgaa ggttgtgcgg cgcggcgtgc tgcctcttcg taggccgaga cagcaaacgc
     8101 gaatactgcc ggcagcgcgg tggacgtgcc gaagtccacc gcctcgtccg tttccggata
     8161 aggcaggtcg gatggccgct ggaacagccc gagcatgatc gcgtcgtgcc ggctggcaaa
     8221 tccgagatcc gccaacgcct cggcctcgat ggcatgcagc agccgccacg agagcggcac
     8281 ctgctggacg atgtagcgtg cagcgatatt gcgcacgatg tgttcaagat cgcgcgccgc
     8341 ggtttgatag cgcagcgcgg ccagagcatg ctcggcttgt tcggtcgtca ggtcgttcat
     8401 tatcggctcc agtcgatacc ctgtactgta caaatataca gtgtctcgtg caacctcggc
     8461 cgaacgttca attcagcacg attccgccaa cagtcagcaa cggtgcgcct gccgctcagc
     8521 catgaatcac caccagcaac gctttcgcct cgcctttgcc ggcgttgcgg attgcgtgcg
     8581 gctcgtcggc gacgtagcgc gcggtgtccg cggccttgag ccgcctggtt gtgcccgccg
     8641 cctcgatctc gatcgatccc tgcaacacgg tcagatgctc ccgcgtgccg ggttcgtggg
     8701 cgttcgacac cagcgccccg cccgcctgca gcgtcaactc ataccactcg aacttgccgg
     8761 ccagttcgat cggcccccac acgcgcaact gatacttcgc ctcgtgcccg ctcaatgtcg
     8821 gaatatcgtg cggaccggcg acggcaatcg cctcgggcgc cttttgcggc gcgaacagcg
     8881 aatcgaggct gacgccgagc gcgttcgtca aacgccaggc gaccgcgatc gtgggattag
     8941 ccttgtcgcg ttcgatctcc gagagcatgg atttcgacac acccgccgcg cgcgacaggt
     9001 cgtcgagcgt catgcgccgt tcggcgcgca gacgctgaat ctgctcgccg acacgaggcg
     9061 gcgccgcggt cgccgggacc gtgcccgtcc ctgaagtggg tgccgcattg gctgctgcgc
     9121 ggcggattcc cgaacttgcc atttactgac ctatcgttta gagttgttcg acataccgga
     9181 ttttagttcg gaaaaacgaa aatatccgat aaaactgaca gccaactata acaggccgcc
     9241 tcgcggtcga acatgccgca agtgccgggc atgtggcccg tgggcgcacc accctttcag
     9301 gagtctggtt catgcgtgac ctttacctcg cccatctgcg cggcactctc gagcagatcc
     9361 gcgccgacgg tttctacaag aacgagcgcg tgatcgccag cccgcagtca gctgacgtgc
     9421 gtctcgcgaa cggcacggaa gtgctcaatt tctgtgcgaa caactatctc ggtctcgcta
     9481 acgacgaacg gctgatcgag gccgcgaagc aaggcctgga tcacgatggc ttcggcatgg
     9541 catcggtgcg cttcatctgc ggcacgcaaa ccgtgcacaa agacctcgaa aaagcgctcg
     9601 ccgcattcct gcaaaccgac gactgcatcc tttactccag ctgtttcgat gcgaacggcg
     9661 gtctgttcga aactttgctc gacgaaaacg acgccatcat cagcgacgaa ctgaaccacg
     9721 cgagcatcat cgacggggta cggctttcga aggcgaagcg ctttcgctat aagaacaacg
     9781 acctcgccga tctggaagcg aaactgaaag aagccgacgc cgctggcgcg cgattcaagt
     9841 tgatcgcaac cgacggcgtg ttttcgatgg acggcatcat tgccaacctc gcaggcattt
     9901 gcgatctcgc cgaccgctac ggcgcactcg tgatggtcga cgattcgcac gcggtcggct
     9961 ttgtcggtga acacggacgc ggcacgccgg aacattgcgg tgtgctgtcg cgcgtcgaca
    10021 tcatgacggg cacgcttggc aaagcgctcg gcggcgcatc gggcggctac gtcgccgcgc
    10081 gccaggaagt ggtcgatttg ctgcgccagc gctcgcgtcc ttatctgttc tcgaacacgc
    10141 taacgccgag catcgctgcc gcctcgctga aagtactcga cctgctcgca agcgacgaag
    10201 gcgcgcaact gcgcgcgcgc gtgcgcgaaa acggcgcgca tttccgcagc gcgatgagcg
    10261 cgctcggctt cacgctcgtg cctggcgaac acccgatcat tccggtcatg ctcggcgacg
    10321 cgcaactcgc atcgaacatg gccgacgcgt tgctgaagga aggcgtgtat gtgatcggct
    10381 tctcgttccc ggtggtgccc aaaggccgcg cgcgaatccg cacgcaaatg agcgccgcgc
    10441 acacgccgga acaaatcgac cgagccgtcg atgcattcgc gcgagtcggc cgtcaactcg
    10501 gtgtgataga acggaggcgc acatgaaagc attggcaaaa ctcgaacgcg cacccggcct
    10561 cacgctgacg gatgtgaaga agcccgaagt cgggcacaac gacgtgatga tccgtatcac
    10621 gcgcacggcg atttgcggta ccgatatcca catctggaaa tgggacgact gggcccaaaa
    10681 aacgatcccg gtgccaatgc acgtcggcca cgaatacgtg ggcgaaattg tcgaaatggg
    10741 ccaggaggtg cgcggctttg cgatcggcga tcgcgtgtcg ggcgaagggc acattacctg
    10801 cggtttctgt cgcaactgtc gtgcgggacg ccggcatctg tgtcgcaata cggtgggcgt
    10861 tggcgtgaat cgtgaaggcg cgttcgctga atatctcgtg attcccgcgt tcaacgcgtt
    10921 caagattccg cctgaaatct ccgacgatct cgccgcgatc ttcgatccgt tcggcaacgc
    10981 cacgcatacc gcgctgtcgt tcaacctggt cggcgaagat gtgctgatca cgggcgccgg
    11041 cccgatcggc atcatggcgg tggcgatcgc gaaacacgtc ggtgcacgca atgtggtgat
    11101 taccgacgtc aacgactatc gcctcgaact cgcgcgcaag atgggcgcca cgcgtgccgt
    11161 gaacgtcgcg cgcgaatcat tgcgcgacgt gatggcggat ttgcatatgg ctgaaggctt
    11221 cgatgtcgga ctggaaatgt ccggtgtgcc aagtgccttc accagcatgc tggaagcgat
    11281 gaaccatggc ggcaagattg cgctgctcgg cattccgccg gcgcagaccg cgatcgactg
    11341 gacccaggtc atctttaaag gtcttgaaat caagggcatt tacggccgcg agatgttcga
    11401 gacctggtac aagatggtcg cgatgctgca gagcggtttg gatttgtcgc cgattctcac
    11461 gcatcatttc aaggtcgacg attatcagga agcgtttgcc acgatgctct ccggcgagag
    11521 cggcaaggtg atcctcgact ggacggctgc ttaagtgaat gagtgaaaaa gcggcgcact
    11581 cgagtgaagc cgccgaaaac aaaacgcctc aaacaaaacg cctcaaacaa aacgcccgca
    11641 tcgtgctcac ccgcctgcgg gcgttttgac gaactcggcc tgaatgcgca catgaatatc
    11701 gtcgccgaca gccgggtacc acgtcgccaa accgaacttc gcgcggctga aatgaccgtc
    11761 cgcggaaaag ccgagcgtgt cctttttcgt gagcggatcg ggtgcgaaac cgttgaaagt
    11821 cacgtcgagc gtgacaggtt gcgtgacgcc gtgaatcgtc aggtcgccgg tcaacatgcc
    11881 acgcgactcg ccggtgcgct caaagcgcgt gctggtgaaa cgcacctccg gatagcgcga
    11941 cacgtccagc atgctcgcgc ttttcaccat cttgtcgagt agcggcacat tcgtgtcgat
    12001 gcttgcggcg tcgatcgtga tcgtcgcgct gctcttgtcg agaccgcctt cgttccagtc
    12061 gagttgcccg tgcttgcggt cgaagcgcat ggtaaagcgc gaatacttga agtgatcgat
    12121 gtcgaaggtg atgctgcaat ggtccggatc gaggtcgtag cgcccaaccg gcacgcgcgc
    12181 ttcgttctga ctgactgtat gtgtgactac ttgcagaggc gtacaggcca ttgcggtcaa
    12241 cagcgttgcg ctgagcagtg cacgcaccaa cgttgcgcgg aaagtagagc gactcatgag
    12301 attcgttcca gttgatgaat caccaccacg atagcagtaa gcgtacccat ccgccgaagc
    12361 gcacaaagac ttgacgaagg agaatgatcg ttctaccctt cacacatggc tactcgaaac
    12421 ccacctgaat tggctgcgtc aggcagcgcg cgtgaaaggc tgctcgatgc cgcggaagca
    12481 ttgatctacg ccggcggcat ccatgcaacc ggtgtcgacg caatcgtcaa gcaatccggc
    12541 accgcgcgta aaagtttcta cacgcatttc gaatcgaaag acgcgctggt cgccgccgcg
    12601 ctggagcggc gcgacgaacg ctggatgaac tggtttatcg cgggcacgct gcagcatggc
    12661 aagactgcgc gcagccatct gctcggcatg ttcgacgtgc tgcgcgaatg gtttgcgtcg
    12721 gcggattttc atggttgcgc gtttctgaat gcggcgggtg aaatcgcctc cgcggaggat
    12781 ccgatccgga tcgtcgcgcg tgaacacaag gaacgcctgc ttgcgttcgt gcgaacccgg
    12841 tgcgatgaat atgcagccga atcgggcacg aacgcccgct gcgccgcgcg cttatcgcgc
    12901 caatggctaa tcctgctcga tggcgccatc gccgtggcgt tggtgagcgg cgaggccgac
    12961 gccgcgctcg acgcgcaggc cgccgccgaa gccttgctcg acaccgaatc cgcaagggaa
    13021 tcacataagc tcacatccaa acccgcgccc agatccgccc gccaactcgc ccgcgaaacc
    13081 gcacccaaac ctgcccgcga acctgcccgc gaacccgccc gcgaacccgc ccgcaaaccc
    13141 gcaagcacat ccccgagcaa acgaccgcca tcccggcgca ccacaacctg actgaggaac
    13201 caccatggct gacccgatcg aaacccgccc gccgctaccc ccgttcacac gcgaaaccgc
    13261 gatccagaaa gtgcgcgccg cggaagacgg ctggaatacc cgtgaccccg agcgcgtgtc
    13321 gctcgcctat acgccggata gtgtgtggcg caaccgtgcc gaattcacgc atggacgtgc
    13381 cgagatcgtc ggactgctgc gccgcaaatg ggccagggaa ctcgactatc gcctgatcaa
    13441 ggaactctgg gcattcacgg ataaccgcat cgccgtgcgc tttgcctacg aatggcacga
    13501 cgattcgaac aactggttcc gttcatatgg caacgaaaat tgggagttcg acgagcatgg
    13561 actgatggcg cgccgtcatg ccagcatcaa cgatctgccg atccgcgagg ccgaccgtct
    13621 ttatcattgg ccgctcggcc gccgtcccga tgatcatccg ggtctgtccg acctcggcct
    13681 ctaaactttt cacggacctt aaagaaggtg gacgcctgcc gcgtgttcac cgtttcgcca
    13741 tcggttctcc gttattgttc tgttttccag tacgcaggac acgtcctgtg cccgcgtgcc
    13801 cgagcgtgtc ttcaacattg cctgtgcggg ttgaggtaca gtgcgcgacg gacggcaagc
    13861 aagaatacct agaacgtgga tgacaccggc gttcgaccgg tgacggagaa cacatgcatt
    13921 ttcggcagat aaagaagcag attcgacaca cggtcgtcgc gcttgtcgcg agcctggctg
    13981 ccgcctccgc ggcgcacgcc ggcgactggt gcgagggcgg cgtatgggtc gacgccatgc
    14041 tcggctcgta tcacatcaac cccgacccaa atacccattt cgagcagttc aatcctggcc
    14101 ttggcgtcga atgctggctc aacaatcaat gggcggtgac tgccggcggc tttcgcaact
    14161 cactgcggcg gccctcgtac tatggcggcg gcgtctgggc gcctgagttc gcgcattggg
    14221 gcatcgtccg gatcgcggcg atcggcggga tcatctcggg atataactac ggcaactggg
    14281 gtctcggtcg caaccacacg atcggaccgg tcgtcgcgcc gatcgtcatg gtcgaataca
    14341 agcgcgtagg ggctaacttc attgtgattc cgccgatccc ctcggacaat cttccattca
    14401 cgatcggctt tcagttgaag gtgaagtttt gatccggctg tcaattcgca aaaaagccgt
    14461 gagttgcgga tttcccttga ttgttcgcaa ataagttgtg agtggcgttg ctacgccttg
    14521 ctgtccggtt aaaaaaactt ggcaaatcgg gacaacagat tgcctggtta tgcgaaagac
    14581 aactcgtgag ctcccgtgac tgagcccggt ttccagcggc ggactacggc tgaaagcgag
    14641 ctcgtggatt tggtcaccta ccttgaaagt gaaggtcaag ctccgacgga acttaccggt
    14701 tgaggcgttt tgtgggctcc tggattcatt caccgtacct gagagtgagg atcacgtttt
    14761 ggtggaactc accggttgag gttgataacg acctcctgga ttttctcacc gcacgtgaga
    14821 gtgaaggtca agttaagatg caactcactg gttgtggtcg ctgaggacct cctggattta
    14881 ctcaccgcac ctgagcgcga aggccaagtg ccggtgcgac tcaccggttg aggcgatagc
    14941 gcctcctgga tttgctcacc gcacgtgatc gctgtgcgtt acggcgaaac tcatgaccaa
    15001 ccattcctcc ggctctgcat ctgggtcact cgaccgtgtc tctgcatgga gtctgatcag
    15061 gccgtactgg gtctccgagg aacgcgggac cgcgtggggg ctgctgatcg cgatcatcgc
    15121 aatggacctg cttctggtcg gcatcaacgc ccggctgaac acatggaacc gcgatttcta
    15181 cgacgcgctg gaaagcagga atgtgcgcga tttcccgcaa ctgatgctga tgtttgccgg
    15241 gcttgcattt gcctccatca cgatctctgt ctacaaccgt tatttgcggc agatgctggg
    15301 cttccgatgg cgccagtggc tgaccacgcg ttacttgaag gaatggttgg gcgacggcac
    15361 cttttaccgc atcgagcgcg accgcctcgt cgataaccct gaccagcgca ttgctgtcga
    15421 cctcgatttg ctcgccaccg ccacactgtc cctcacgctc gacctgctct cgacgattga
    15481 gacgctcgtc tggttctcca tcgtcctgtg gagcacggcg ggcgcgctgg tcatcatgat
    15541 gggaggcacg cccgtccaga ttcagggcta catgttctgg gcggccgtcg cctatgcgat
    15601 cgtcggctcg ctcctcacca ataaggtcgg tcacccactc gtgtcgatca actatcagtt
    15661 gcaacgtgtc gaggcggatt tccggttcgg cctgatccgt ctgcgcgaaa acgcggagca
    15721 gattgcgctt tatgacggca tgcggaccga ggcatcgaac gccgaaaatc tgttcggccg
    15781 gattcgtgaa aactggtggc gcctcatgag atatacgaag cgctacagtt tcgtggtcaa
    15841 cttctacggt cagatcgccg agatctttcc gatcgtcgtt gcatcgcccc gctatttttc
    15901 aggcgcgttg agtttcggca cgctgatgca gatcgccgac gcattcggtt cggttagcga
    15961 atcgctttcg tggttcatca acaattacga cacgctcgtc gaatggcgtg cgacggttaa
    16021 ccgtctgggc gagttcaagc gtgttgtgag gcaacctcac ctgaaggaat ctgtgtcgcc
    16081 tgccaccgag catggcggca tcaaactgca ctacgtcgac gagaaccagc tcttcacgca
    16141 cgacctcatg cttgcgctgc ctaacggcga aacgctcgcg agcgtgcccg acatcgtcgt
    16201 aaaacccggt tcgcggtggc ttgtgcgcgg accgtccggc tcgggcaaga gcacgctatt
    16261 gcgagcgctg gctggtctct ggccgttcgg cgacggctcg atcgatgcac ccgtggacgc
    16321 gcgcatgatg ttcatcccgc agcagagcta tctgccagtg ggcacgttga aagctgcgct
    16381 ggcgtaccca acgccggccg aatccttcag cgacgacgac tgccgcgagg cactacgtga
    16441 gtgccggctg gaagagtatg ttggccgatt gcaggagtcc gaccactggt ggcgcattct
    16501 ttccccgggc gagcagcaga ggctagcggt cgcgcgcgtt ttgctgcaca agcctgacta
    16561 cgtgttcctc gacgaagcaa cgagcgcgct tgacaccgaa aacgaagcgc atctttaccg
    16621 cctcctcacc gaacgactac ctaaggcggc gatggttagc gtcgcgcagc acgagtcgct
    16681 tgtgcaattt ggccaggaga cgctcgatat caggcgtccg tgaaaaaagg ggggccgtga
    16741 taaatctgcg acccatggca tcgcatgagc cggaagcgga taacgtaaag cgtagcagga
    16801 caaatgggac caaaggccaa ttgccatcga acggcgcttt ccaaggccac cacgctgtat
    16861 gcgcaggtgg gctacgtgga caaccgtggc aagatgaatc agtcgatcga gtatggacag
    16921 cctgtcgcgc cgggtgccaa tacgacggcg gcgatggtgg gcatcagaaa gcggttctga
    16981 cggctgctgc gcgggtgcca ctgaaagtcg cacatttgcg cgatcgacct cctatgatgc
    17041 cgaatgttca ggcagttctc taacggactc gatcacccgg caaaggatgg catccatggt
    17101 tggttcagag cggcgtgtgt ccgtcgtgac aggtgcgacc ggcgggattg gacgctggat
    17161 cgcgctcggg ctcgcgcagg ccgatcacca cgtggtactg gtctgcaggg atgcggtgaa
    17221 agggcgcgcg ctcgccgact ggatcagcca gcaagcgcct gctgcgagca cggagctgag
    17281 actcgccgat ctgagctcgc tttgcgcggt acaacgtctc ggtcaggaga tcgccgctgt
    17341 ccatccacgg ctatccgttc tcatcaataa cgcgggcatg ttcaccgcgc gccggtcgac
    17401 gacggcggaa ggccacgaca tcgtgctggc gctcaaccat ctcgcgccgc tcgtgctgac
    17461 tgacgcgctt gagccggcgc ttcgggctgg aagtcccgcg cggatcgtga acgtgggttc
    17521 gtcgacttcg gagcgcgcgc gaatcgatcc ggacgacctc gaactcgttc ggcactgggg
    17581 catggtgcga gcctacggac gttcaaaact ggcgttgatg atgtcgacgt tcgcgcgggc
    17641 gcaacgtctg cgcggcagcg gcgtggtggc gaacgtcgtg catcctggga ccgtagcgac
    17701 tggtctggtt cgcgaaggcg gcgcaatcgg cgccgcgtgg cggctcatgg gaccgttcct
    17761 gcgcaccggg cagcagggcg cggacacacc gctccatgtc gccttggcgc cggcctgggc
    17821 ggcagccact ggcctgtatg tcaaagaccg cgctgcagtc cgtccgaacc ggcgagcgct
    17881 ggacccggct ctcgtggaaa aggtcgaagc cgcttcaaga gagttggcgg gacgcaccac
    17941 cgtcgtggat ataccgctct aggcgagcgc gcgacccatg gcccgcaccg ctcaatccag
    18001 agcatgctcc tgaaccgtcg tttcatccgc ggggcggtca ggcgaagcct gccttccggc
    18061 ctgcggttcc caacgggcat gctgccgcag ccgcgcgagc atactcagga tacggatgtg
    18121 aacgctgccc gaacgtgcct gagcgtcgaa cgcgccccgc acgggtgagg ggcgatcgga
    18181 agcgtcgaac aaggtgggcg cgtgccgctc cttcagcgcg gcctcatccg tcagctggcc
    18241 gttgtagctg agcttgcgta tggccgcggc gacaagtccc ggcacaagtc gaacgccgag
    18301 cgagaacaga atcgccgttg aggtcaccgg catctccgca cgtccgctga ttagcgcgag
    18361 gcggatggcc tcaacgacga cctccggctg gtagaccggc gacatcggcc gcccgagttg
    18421 acccatgtgt gatatcgcgt ggtcgaagaa cggtgtgttc accgcgggtg gaaacacgct
    18481 tgtcaggcgc acgtggctgc cgtcctggga cagctccgcg cgaatcgact ggccgaagcc
    18541 gcgcaccgcg tgctttgcgc cgctgtaggc ggacagcagc ggcatgccat gaaacgcgac
    18601 cgcggaacag acgttgacga ttgagccgcg atcgcgcggt atgaaccgcc gtaatgcgac
    18661 tcgcgtgccg ttgaccgtgc ccatgtaggt gacgtcggtg acgcgccgga actgttcggc
    18721 gggggtatcc agaaagcggc cataagtgcc gttgccggcg cagttgatcc atgcatcgat
    18781 ggggccgagt actgcctcga tgcgggcggc tgctgcttcc aatgcgtcaa gatcggtcac
    18841 gtcagcctgt tccatcgcgg ccatggcgcc gtggcgatgc acatcgtcga agactgcctc
    18901 caaacctgcc cgcccgcggg caatgaggcc gacccgccaa ccatatcggg cgaagaagtt
    18961 ggccgtgccg cgcccaatgc ccgaggaggc gccggtgatc actatcacgc gcgcctcagt
    19021 tcgttggttc ggacttgcgt ggtggctgga ttgcatcagg aagtcggggt cgctcggcat
    19081 tcagatatcc ctgtcggtct gcgtgctgtc aatgctcgct aactcgccct ttagccttga
    19141 gagagttgca gcgtcatgcg gcaatgatgc gcggactcgc gcccgtgatg ccggggctct
    19201 atcgctctaa cggtgagcag aaatccccgg cacagaatat ctcaagccgg tggttctcga
    19261 tattagacct tgggccaatg gagccttgca atggagcctt gttttgcggg gcgtttgtaa
    19321 ggaagcctct gttctaaccc ctgcagcgga aaactgcgtt cgccgtcttc ggccgctcag
    19381 gcgacttcat cggctttccg tttggtacga ttggcgcaat tagccttggg cctggcggaa
    19441 aaagccgctt gcgagttcgt gttggattgc gctgaaaata ttcgctaccg cttcggcatc
    19501 cagcgcgcta taacaggtat ttgcagggcg attattacgt gtcgaagggt ccacaaaatc
    19561 cgttgacact gccagttcgc acgccacctt gcgccccggg acctcggctc ggcgcactgc
    19621 tctccgcatg ttcatcacga cctaaaactt tggcctcggg caattcggcc gtggacccga
    19681 agcgcgtctt cgcgcgtccg aaaatccctt atcaaggttg acttccgggc atgcggctaa
    19741 ctattcgcac gagtgaaaac tgcaccctga atcaaggagc taaacgtgaa aacacgacta
    19801 ttggtggctt ctgccgccgg attgcttgtc atggccggct ttgcgcattc gcaaaacatg
    19861 gcggagcctc agcaacccac ttcacaggaa aacgcagcga ctcagcctgc cgcctcgccg
    19921 tccgacaccg ggacgcaggc atacggcggt acgccggata cgaaaatgca gagcggttgg
    19981 aaacatatga agtcctgcag caccgatccg caatgcaata tcttcttcgg tgggagttaa
    20041 aagcaaactg gtgtcgcgcc ggacaatagc ggcaaacaaa acgggcgcgg gccggaaggc
    20101 gtgacaacgt cagccgtcat gcgcgaccgt ccagtcaaag gcaggtcatt tccagaatgg
    20161 tgagggattc caggagacgg cgctatctcg cccaacggtg aggaattcca ggagatggcg
    20221 ccacctcgct caacggtgag gaattccagg agacggcgcc acctcgctca acggtgagga
    20281 attccaggag acggcgccac ctcgctcaac ggtgaggaat tccag
//
LOCUS       DATASH010000023         7862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4948296,
            whole genome shotgun sequence.
ACCESSION   DATASH010000023 DATASH010000000
VERSION     DATASH010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7862
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4948296"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2825)
                     /gene="ppc"
                     /locus_tag="VHV99_00710"
     CDS             complement(<1..2825)
                     /gene="ppc"
                     /locus_tag="VHV99_00710"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEX3634016.1"
                     /translation="MTSSGSARPARRNTASPNAQAAAAAAAPAASASAATAAQSTPAK
                     AAGKAAKASQAANVAKSAKSAKASAPARTEKPVKAAKAVKTSKAAQAAKAAQAVKPAK
                     AKSPKIKVIANEAAVPQTAKAPRLEAVSPDTVVPASKSSGRTREDKDHPLFQDIRYLG
                     RLLGDVLREQEGDAVFDVVETIRQTAVRFRREDDSAAAQTLEKKLRTLSPEQTVSVVR
                     AFSYFSHLANIAEDRHRNRRHRIHALAGSTSQPGTMAYALERLVEAGAAATPVLQQFF
                     NDALIVPVLTAHPTEVQRKSILDAQHDVARLLAERDQQLTNRERARNEAMLRASVTSL
                     WQTRMLRDSRLTVADEIENALSYYRATFLEEIPALYADIEEALAEHGIAARLPPFFQM
                     GSWIGGDRDGNPNVTAETLEHAITRQAAVIFEHYMEQVHKLGAELSVSNLLAGASDAL
                     KDLAAASPDQSPHRTDEPYRRALIGMYTRLAASARVRLGEGSVPVRSAGRGAAPIRAQ
                     PYDDADEFVRDLHVLIDSLAEHHGAPLAAPRLSPLARAAEVFGFHLASIDLRQSSDIH
                     EAVIAELLKRAGVEADYASLAEEDKLKVLLAELAQPRALRLPYAEYSDLVKSELGVLE
                     EARVTREKFGARAVRNYIISHTETVSDLVEVMLLQKETGLLHGRLGDANDPARAGLMV
                     IPLFETIPDLRNAPHIMRDLIALPGVGALIEHQGNEQEVMLGYSDSNKDGGFLTSSWE
                     LYRAELALVSLFNERGVTLRLFHWRGGTVGRGGGPTYQAILSQPPGTVDGQIRLTEQG
                     EVIASKFGNPEIGRRNLETVVAATLEASLLPHGNAPAQLPAFEETMQQLSDAAMASYR
                     ALVYETPGFKEYFFESTPIAEIAELNIGSRPASRKLQDPKQRKIEDLRAIPWGFSWGQ
                     CRLLLTGWYGFGSAVAAHLDGAPSDAER"
     gene            3285..4319
                     /gene="hemC"
                     /locus_tag="VHV99_00715"
     CDS             3285..4319
                     /gene="hemC"
                     /locus_tag="VHV99_00715"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEX3634017.1"
                     /translation="MNTETFFAPPHTLVIASRESRLAMWQAEHVQCALHKLYPSCDVK
                     ILGMTTRGDQILDRTLSKVGGKGLFVKELEAALADGRADLAVHSLKDVPMELPAGFAL
                     SAIMEREDPRDALVSNHYDSLAALPAGAVVGTSSLRREAMLRMRYPHLVVRPLRGNLD
                     TRLSKLDRGDYAAIILAAAGLKRLGLGARIRALLDPEDSLPAAGQGALGIEIRADRPE
                     LAAWLAPLHHEPTAAAVEAERMVSRALGGSCEVPLAAYAHWHDGALHLRGIVATPDGQ
                     RVLSAQASAPAPGVERAVALGQEVASALEQQGAMDIVRALSAASGPAAEAGADAKAAD
                     GASDSAAPRE"
     gene            4319..6340
                     /gene="hemDX"
                     /locus_tag="VHV99_00720"
     CDS             4319..6340
                     /gene="hemDX"
                     /locus_tag="VHV99_00720"
                     /EC_number="2.1.1.107"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fused uroporphyrinogen-III synthase
                     HemD/membrane protein HemX"
                     /protein_id="HEX3634018.1"
                     /translation="MAADIPGNASSSVGKAAFTVVITRPAGQSNELIAQLAAADVAVL
                     DFPLIDIARVTDDAPLRAALASLERYALVVFVSPNAVDHAFARRDAIWPHALPIGVVG
                     PGSVQALVRHGVTAPAYNVISPPSVPDDDTGRFDSEGLFAALDAALGASTLEGKRVLI
                     VRGDGGREWLADRLREAGAEVETVAAYRRLVPEPSIGGWSRVHKLLAGEPHAWLLTSS
                     EGVRNLHDLAQEHLTVDEITQLKHAALITPHPRIAETARALGFDSITVSGAGDERIVR
                     ALLAAVPTVVQPAPSNPAHSPAKSRMTETNASTNASPQPAATAALPPNQPFTPYEAQQ
                     KRRSASGPLLWFVVVIIACAAGVGGYALNRKVERTEQQLVQRQQANDAQTNELRVKTD
                     QALATVHQSDSQVAQLEGKLADAQSAQQALQQQYADLARNRDDWTMAEVGQMLSAASQ
                     QLQLTGNTQLALFALQSADTRLAASDSPQAVAVRKAIAQDIDKLKAAPSTDLTGMAIK
                     LDNAIDQVDTLPLSGEAPIAHATPKAATWDDTAKVAAATGEPRWKVWWREVTTGIGQQ
                     LTSLVQVRRIDNADAMLITPDQGYFVRENLKLRLLSARLALLSRNQTTLKSDLQATQT
                     ALTRYFDSTSKKTQTVIDLLKQVDAGSAAVELPNLSTSLQAVNQYRSRG"
     gene            6343..7533
                     /locus_tag="VHV99_00725"
     CDS             6343..7533
                     /locus_tag="VHV99_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HEX3634019.1"
                     /translation="MAIRGLLWLALLFAIAVVLAVVGRFDMGQVLLIYPPYRVDISLN
                     LFVVGLVVLFILLYALLRIVRNIWRMPQRVAAYRARSRVAKAHAALRDAIGNLYAGRF
                     SRAEKAAKDSLTNGANKGAAGLIAATAAHRMHEYARRDEWLAQIDEADWQDARLMATA
                     DMRADGRDADGALTALTEMQSQGARRIHAQQIMLRAQQQLKNWGEVLKLVKTLEKREA
                     IHPAVAVRLRQLAAENLLRDRRHNADALLELWNSLSATERHSPRLADLAAELLVSLNR
                     PQEARKIVEEALAQNWDARLLRRYPDTTGGDALPLIQKAEGWQKDRPEDADLLFALGR
                     LCLHQQLWGKAQSFLERALKLADNETLKIRSHRALARLHEQLGDADKASQHYRESALA
                     MNIV"
BASE COUNT         1358 a         2515 c         2609 g         1380 t
ORIGIN      
        1 cgctcggcgt cgctcggcgc gccgtccaga tgcgcggcca cggcgctgcc gaaaccgtac
       61 cagccggtca gcagcaaacg gcattggccc catgagaaac cccacggaat cgcgcgcaga
      121 tcttcgatct tgcgttgctt cggatcctgc agcttgcgcg aagccggccg gctgccgatg
      181 ttcaactcgg cgatctccgc aatcggcgtc gattcgaaga agtactcctt gaagcccggc
      241 gtttcataga ccagcgcacg gtaggacgcc atcgccgcgt cggacaactg ctgcatggtt
      301 tcttcgaagg cgggcagttg ggccggcgca ttgccgtgcg gcagcaacga cgcttcgagc
      361 gtggcggcga ccaccgtctc cagattgcgc cggccgattt ccggattgcc gaacttgctc
      421 gcgatcactt cgccttgctc ggtcaagcgg atctggccgt cgacggtgcc cggcggctgc
      481 gacaggatcg cctgataggt cggaccgccg ccacggccga cggtgcctcc gcgccaatgg
      541 aacagccgca gcgtcacgcc gcgttcgttg aagagcgaca ccagcgcaag ttcggcgcgg
      601 tacaactccc agctcgacgt gaggaagccg ccgtccttgt tgctgtccga atagccgagc
      661 atcacttcct gctcgttgcc ctgatgttcg atcagcgcgc cgacgcccgg cagcgcgatc
      721 agatcgcgca tgatgtgcgg cgcgttgcgc aagtcgggga tcgtctcgaa cagcggaatc
      781 accatcaagc ccgctcgtgc cggatcgttt gcatcgccga gacggccgtg aagcagaccg
      841 gtttccttct gcagcaacat cacttcgacc agatcgctga cggtctccgt gtgcgaaatg
      901 atgtagttgc gcactgcgcg cgcgccgaac ttctcgcgcg tgacacgggc ctcttcgagc
      961 acgccgagtt cgctcttcac gagatcggag tactcggcgt acggcagacg caacgcgcgc
     1021 ggctgcgcca gttcggcgag caacaccttg agcttgtctt cttcggcgag cgacgcgtag
     1081 tctgcttcga cgcccgcgcg tttcaacagc tcggcgatca ccgcttcatg gatgtccgag
     1141 ctctggcgca agtcgatact cgccagatgg aagccgaaca cttcggcggc acgcgcgagc
     1201 ggcgacaggc gcggtgccgc gagcggcgcg ccatgatgct cggcgagcga gtcgatcagc
     1261 acgtgcaggt cgcgcacgaa ttcgtcggcg tcgtcgtacg gttgggcgcg gatcggcgcc
     1321 gcgccgcggc ccgcgctgcg caccggtacg ctgccttcgc cgagccgcac gcgcgcactt
     1381 gccgcaagcc gcgtgtacat gccgatcaag gcgcgccgat acggctcgtc ggtacggtgc
     1441 ggcgactggt cgggcgaggc ggccgcgagg tctttcaacg cgtcgcttgc gccggccagc
     1501 aggttcgaca ccgacaactc ggcgcccagc ttgtgcacct gctccatgta gtgttcgaag
     1561 atcaccgcgg cctggcgggt gatcgcgtgc tcgagcgttt cggccgtgac gttcggattg
     1621 ccgtcgcggt caccgccgat ccagctgccc atctggaaga acggcggcag acgcgcggcg
     1681 atgccgtgct cggcgagcgc ttcctcgata tcggcgtaaa gcgccggaat ttcttcgagg
     1741 aaggtggcgc ggtagtacga cagcgcgttt tcgatttcgt cggccactgt caggcgcgag
     1801 tcgcgcagca tccgcgtttg ccacagcgac gtgacacttg cgcgcagcat cgcttcgtta
     1861 cgcgcgcgtt cgcggttggt caattgctgg tcgcgttcgg cgagcaggcg cgcgacgtcg
     1921 tgttgtgcgt cgagaatgct cttgcgctgt acttcggttg ggtgcgcggt caggaccggc
     1981 acgatcagcg cgtcgttgaa gaactgctgc aacaccggcg tggcggcggc gcccgcctcg
     2041 acgagccgtt cgagcgcata cgccatggtg cccggttgcg aggtcgaacc ggccagcgcg
     2101 tgaatccggt gacggcgatt gcggtgacgg tcctcggcga tattcgcaag atgcgagaag
     2161 tagctgaacg cgcgcaccac gctgaccgtt tgctcggggc tcagcgtgcg cagtttcttc
     2221 tcgagcgttt gcgcggcggc actgtcgtcc tcacggcgaa agcgcacagc ggtctggcgg
     2281 atcgtctcca cgacgtcgaa caccgcgtcg ccttcctgtt cgcgcagcac gtcgccgagc
     2341 aggcgtccca gataacggat gtcctggaac agggggtggt ccttgtcctc acgggtgcgg
     2401 ccgctgcttt tcgatgccgg cacgaccgta tccggcgaaa cggcttccag cctgggcgcc
     2461 ttcgcggttt gcggcacggc ggcttcgttc gcgatgacct tgatcttcgg ggatttggct
     2521 ttggccggtt tcaccgcttg agcggccttc gcggcttgcg ctgccttgga tgtcttgacg
     2581 gccttcgccg ccttcaccgg cttttccgtc ctggcgggcg cggacgcttt ggcggacttg
     2641 gcggatttgg cgacattcgc ggcctggctt gccttggcgg ctttgccggc agctttggcg
     2701 ggtgtggatt gggcagccgt tgcggcggag gcagaagcgg cgggcgccgc ggcggcggcg
     2761 gccgcttgag cgttgggcga tgcagtgttg cggcgggcag ggcgcgccga tccggaagac
     2821 gtcacgatgg gtttcctcag ggaagccagg gtctgttgag tgatgaactg ctactgcgga
     2881 actgcactat gtgttgcaac gtgaactgca cgaaaccagc acaaagcggt acgcgccgat
     2941 gggcaccacg ccggagagga tggccgcgcg cgcatttgca caggcgcaca aagcgcacat
     3001 gcaaggcgca caaagcacac aaagcgcttg aggcgcacga cgtgcatttg catgcgccat
     3061 aggcacgcga aagcacgttg cgccgcattg catcatcccg catgccgtgg cccggtgcgc
     3121 gaacgaccgt gctcgaacag gctggaatga ggccggcgcg cgccgcgcga gacccgtctc
     3181 ctccatcgat accgcgccag cacggtgttc cgctgtgcct tgagacacag ggtcgcgtgc
     3241 taacattgat tgttattaca tcccgtcatt cgatcagcaa gctaatgaac accgagacgt
     3301 tttttgcgcc accccacacg cttgtgattg catcgcgaga aagccgcctt gccatgtggc
     3361 aggctgagca tgtgcaatgt gcgctgcaca aattatatcc atcttgtgac gtaaaaatcc
     3421 ttggcatgac gacacgtggc gatcagattc tcgatcgcac tttgtcgaag gtcggtggta
     3481 agggcctctt cgtcaaggaa ctggaagcgg cgctggccga cggccgcgcc gacctggccg
     3541 tgcactcgct caaagacgtg ccgatggaat tgcccgctgg ctttgcgctc tccgccatca
     3601 tggagcgcga agatccgcgc gacgcgctgg tgtccaacca ctacgactcg ctggccgcgt
     3661 tgccggccgg cgccgtggtg ggcacctcca gtctgcgccg cgaggccatg ctgcgcatgc
     3721 gttacccgca ccttgtggtg cggccgttgc ggggcaatct ggacacccgc ctgtcgaaac
     3781 tcgatcgcgg cgactacgcg gcgatcattc tggcggcggc cggcttgaag cgtctgggtc
     3841 tcggcgcgcg catccgcgcg ctgctcgatc ccgaagacag cttgcccgca gccggtcagg
     3901 gcgcgctcgg catcgaaatt cgcgccgatc gcccggagct cgcggcatgg ctcgcgccgc
     3961 tgcatcacga acctacggca gcggccgttg aagccgagcg gatggtctcg cgcgcgcttg
     4021 gcggcagttg cgaagtgccg ctcgcggctt atgcgcactg gcacgacggc gcgctgcatc
     4081 tgcgcggcat cgtcgccacg cccgacggac agcgggtcct gagcgcgcag gcgtccgcgc
     4141 cggcgcctgg cgtcgaacgt gctgtcgcgc tcggccagga agtggcgagt gcgcttgaac
     4201 agcaaggcgc gatggacatc gtccgcgcgc tgagcgcggc cagcggtccc gcggcagagg
     4261 caggggcaga cgcaaaggca gccgacggcg cgtcggacag cgcggcgccc cgcgagtgat
     4321 ggcggccgat atcccgggca acgcatcctc atccgtcggc aaggcggcgt tcacggtcgt
     4381 gattacgcga ccggccggtc agtcgaatga attgattgcg caactcgcgg cggcagatgt
     4441 cgccgtgctc gactttccgc tgatcgatat cgcgcgtgtc actgacgacg cgccgttgcg
     4501 cgcggcgctg gcttctctgg agcgctatgc cctggtggtg ttcgtctcgc ccaatgcggt
     4561 cgatcacgcg tttgccaggc gcgatgcgat ctggccgcac gcgctgccga tcggcgtagt
     4621 cgggccgggc agtgtgcagg cgctggtgcg ccacggcgtc acggcgccgg catacaacgt
     4681 catcagcccg ccgtccgtac ccgacgacga caccgggcgc ttcgattccg aaggcctgtt
     4741 cgcggcgctc gatgccgcgc tcggcgcgag cacgctagaa ggcaaacgcg tgctgatcgt
     4801 gcgcggcgac ggcggccgcg aatggctcgc cgaccgcttg cgcgaagcgg gcgccgaagt
     4861 cgaaacggtg gcggcctatc gccgcctcgt gccggaaccg tcgatcggcg gctggtcgcg
     4921 cgtgcacaag ctgttggcgg gcgagccgca tgcctggctg ctcaccagtt cggaaggggt
     4981 gcgcaatctg cacgacctcg cgcaagagca cctgaccgtc gacgaaatca cacaactgaa
     5041 gcacgccgcc ctgatcacgc cgcacccccg tatcgcggag actgcgcggg cattgggttt
     5101 tgatagcatt acggtgtccg gtgcgggcga tgaacgcatc gtccgcgccc tgcttgccgc
     5161 cgtcccgacc gtagttcaac cggcaccgtc caacccggct cattcaccgg ctaaatcacg
     5221 catgactgaa acgaacgcct ccacgaacgc ttcacctcag ccggccgcga ccgcagcgtt
     5281 gccgccgaat caacccttca cgccctacga agcgcagcaa aagcgccgca gcgcgagcgg
     5341 accgctgctg tggtttgtcg tcgtgatcat tgcgtgtgcg gcaggcgtgg gcggctatgc
     5401 gctcaatcgc aaggtggagc gcaccgagca gcagctcgtg caacgccagc aggctaacga
     5461 cgcgcagacc aacgaactgc gcgtcaagac cgatcaggcg ctggccacgg tgcatcaatc
     5521 cgactcgcag gtcgcgcaac tcgaaggcaa gctcgccgac gcgcaaagcg cgcagcaagc
     5581 gctgcaacag caatacgccg atctcgcccg caatcgcgac gactggacga tggctgaagt
     5641 cggccagatg ctgtcggccg cgagccagca attgcagctc accggcaata cgcaactcgc
     5701 gctgttcgcg ctgcaaagcg cggacacgcg gctcgctgcg tcggatagcc cgcaggccgt
     5761 ggccgtgcgc aaggcgatcg cgcaagacat cgacaagctg aaggccgcgc cctccacgga
     5821 tctgacgggt atggcgatca agctcgacaa cgcgatcgat caggtcgaca ccttgccgct
     5881 ctccggcgaa gcaccgattg cccacgccac gccgaaggcc gccacctggg acgacacggc
     5941 gaaggtggcc gcggccaccg gcgagccgcg ctggaaagtc tggtggcgcg aagtcaccac
     6001 cggcatcggc cagcagttga cgagcctcgt gcaggtgcgc cgcatcgaca acgccgacgc
     6061 catgctgatc acgcccgatc aaggctactt cgtgcgcgag aatctgaagc tgcgtttgct
     6121 gtcggcgcgg ctggcgctgc tctcgcgcaa ccagaccacg ctgaagtccg acctgcaggc
     6181 gacgcagact gcgctcacgc gctacttcga cagcacgtcg aagaaaacgc agaccgtgat
     6241 cgatctgctc aagcaggtgg atgcgggctc ggccgcggtt gaactgccga atctgagcac
     6301 cagtctgcag gccgtcaatc aataccggag ccgaggttaa tcatggcaat ccggggactt
     6361 ctatggctcg cgttgctgtt cgccatcgcg gtggtgctgg cggtggtcgg acgcttcgac
     6421 atggggcagg tactgctgat ctatccgccg taccgcgtcg acatctcgct gaatctgttc
     6481 gtggtcgggc tggtggtgct gttcatcctg ctgtatgcac tgctgcgcat cgtgcgcaat
     6541 atctggcgca tgccgcagcg cgtggcggcc tatcgcgctc gctcgcgtgt cgcgaaggcg
     6601 cacgccgcat tgcgtgacgc gattggtaac ctgtacgccg ggcgtttctc gcgcgctgaa
     6661 aaagccgcga aagactcgct cacgaatggc gccaacaagg gcgcggccgg tttgatcgcg
     6721 gccactgcgg cgcaccgcat gcatgaatat gcgcggcgcg acgaatggct cgcccagatc
     6781 gacgaggccg actggcagga cgcacgcctg atggccaccg ccgacatgcg ggccgacggc
     6841 cgcgacgcgg acggcgcgct caccgcgctc accgaaatgc agtcgcaggg cgcacggcgc
     6901 attcacgcgc agcagatcat gttgcgcgcg caacagcaat tgaagaactg gggcgaagtg
     6961 ctcaagctcg tcaagacgct ggaaaagcgc gaggcgatcc atccggcagt ggcggtgcgc
     7021 ctgcgtcaac tggcggcgga aaatctgctc cgcgacaggc gtcataacgc cgatgcgctg
     7081 ctggagttgt ggaattcgct gtcggccact gagcgtcatt caccgcgact cgccgatctc
     7141 gctgctgaat tgctggtgtc gctgaatcgt ccgcaggaag cacgcaagat cgtagaagaa
     7201 gcgctggcgc agaactggga cgcgcggctg ctacgtcgtt atccggatac gaccgggggc
     7261 gacgcgctgc cgctgatcca gaaggccgaa gggtggcaga aggatcgtcc ggaagacgcg
     7321 gacttgctgt tcgcattggg ccgcttgtgt ctgcatcagc agttgtgggg caaggcgcaa
     7381 tcgttcctcg aacgcgcgct gaaactggcc gacaacgaga cgctgaagat tcgttcgcat
     7441 cgcgcgttgg cgcgtttgca tgaacagctc ggcgatgcgg ataaggcgag ccagcattat
     7501 cgtgagagcg cgttggcgat gaatatcgtt tgatgctttg agtggggcgg cgcgcggttt
     7561 ttgaaacgct cagacggtgc gcaggaatca gagcttgcaa tgaagaagcc ccggcgtcgg
     7621 aagacgtcgg ggcttttctt ttgaacctgc ggcttgcggg gtgagccgtg gaccgcgaac
     7681 tatgagccac gaaccacgaa ccactgtgca tgcggtgtga actcaaaaag atgaactgtg
     7741 acgcgtcact caggacgttt gacggcggct cctgcgcgag cgcatcgacg agttcatcgg
     7801 tcagccaagc tacagcgatt taaagctact tattgaccat cttcgcgggc acgctgatcg
     7861 cc
//
LOCUS       DATASH010000024         1572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_398721,
            whole genome shotgun sequence.
ACCESSION   DATASH010000024 DATASH010000000
VERSION     DATASH010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398721"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1572
                     /locus_tag="VHV99_00730"
     CDS             <1..>1572
                     /locus_tag="VHV99_00730"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HEX3634020.1"
                     /translation="SELVQDPVDEASRLTPLGLPHAKIALPRQLYGSERLNSMGFDLS
                     NEHRLASLSSALLASANHPWRAAPMLEATDVVEGVARDVRNPADLRDLVGTVIEATSE
                     QVALAVSNAVAAAPIWQSTPVDERADCLARAADLLEAQMHTLMGLVVREAGKSLANAV
                     SEIREAIDFLRYYSAQIRSEFSNDTHRPLGPVVCISPWNFPLAIFMGQVAAALAAGNP
                     VLAKPAEQTPLIAAQAVRILREAGVPPGAVQLLPGNGETVGAALVADPRTRAVMFTGS
                     TEVARLINKTLSERLDPDGRPIPLIAETGGQNSMIVDSSALAEQVVADVLQSSFDSAG
                     QRCSALRVLCLQDDVADRTLEMLTGAMQQLAMGNPDRLSTDVGPVIDAEAKQSIDAHI
                     ASMREKGRNVVQLPMPEGCAQGTFVPPTLIELDSIDELQREVFGPVLHVVRYRRAHLD
                     KLLDQIKATGYGLTLGIHTRIDETIAHVVSRAHVGNIYVNRNVIGAVVGVQPFGGEGL
                     SGTGPKAGGPLYLQRL"
BASE COUNT          274 a          477 c          516 g          305 t
ORIGIN      
        1 cagcgagctc gtgcaagatc ctgtcgacga agcgtccagg ctcacgccgc tcggtctgcc
       61 gcacgcgaag attgcgctgc cgcgtcagtt gtacggtagc gagcgcctca attcgatggg
      121 cttcgatctg tcgaatgagc atcggctggc gtcgttgtcg tcggcgttgc ttgcgagcgc
      181 gaatcatccg tggcgcgcgg ctccgatgct ggaagccact gacgtagtgg aaggtgtcgc
      241 acgcgacgtt cgcaatcccg ccgatctgcg cgacttggtc ggtacggtga tcgaggcgac
      301 atcggagcaa gttgccttgg ctgtttccaa cgccgtcgcc gccgcgccga tctggcaatc
      361 aacgcccgtc gatgaacgcg ccgattgcct cgcccgcgca gccgatctgc tcgaagcgca
      421 gatgcatacg ctgatgggcc tcgttgtgcg cgaagcaggg aaatcgctgg cgaacgcggt
      481 gtcggagatt cgcgaggcta tcgacttcct gcgttattac tcggcgcaaa tccgcagtga
      541 attttccaac gatacccatc gtccgctcgg ccccgtggtg tgcatcagcc cgtggaactt
      601 cccgctcgcg atcttcatgg gacaagtggc ggccgcgctc gctgccggta atccggtgct
      661 cgcgaagccg gctgaacaga caccgttgat cgcggcgcaa gccgtgcgca tcctgcgcga
      721 agccggcgtg ccgccgggcg cggtgcaatt gctgccgggc aatggcgaaa ccgtgggcgc
      781 ggcgctggtc gccgatcccc gtacgcgtgc cgtgatgttc accggttcga cggaagtcgc
      841 gcgcctgatc aacaagacct tgtccgaacg cctcgacccg gatggccggc cgattccgct
      901 gattgcggag acgggcggtc aaaattcgat gatcgtcgat tcgtcggcgc tggccgagca
      961 agtggtcgcg gacgtgttgc aatcgtcctt cgattccgcc ggtcaacgct gttccgcgct
     1021 gcgcgtgtta tgcctgcagg acgacgtcgc ggaccgcacg cttgaaatgc tcaccggcgc
     1081 gatgcagcaa ctcgcgatgg gaaatcccga ccggctttcc accgatgtcg gcccggtgat
     1141 cgatgctgaa gcgaagcaaa gcatcgacgc gcatattgca tcgatgcgcg agaagggccg
     1201 caatgtcgtg cagttgccca tgcccgaagg ctgcgcgcag ggcacgttcg tcccgccgac
     1261 gttgatcgaa cttgattcca tcgatgagtt gcagcgcgag gtcttcgggc cggtacttca
     1321 cgtagtgcgg tatcgccggg cacatcttga taaactgctc gaccaaatta aagcgacggg
     1381 ttacgggctg acgctgggta tccatacgcg tatcgatgaa accatcgcgc acgtggtcag
     1441 ccgggcgcac gtgggcaaca tctatgtgaa ccggaacgtg attggcgcag tggtcggcgt
     1501 gcagccgttc ggcggcgaag gtttgtcggg cacgggtccg aaagcgggtg gtccgctgta
     1561 cttgcagcgg ct
//
LOCUS       DATASH010000025        10496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4750650,
            whole genome shotgun sequence.
ACCESSION   DATASH010000025 DATASH010000000
VERSION     DATASH010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 10496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10496
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4750650"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..152
                     /locus_tag="VHV99_00735"
     CDS             <1..152
                     /locus_tag="VHV99_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX3634021.1"
                     /translation="GYRGATTNVEKMETRVKFDLFYIHNWSFWFDMKIVFITIFKGFV
                     GRNAF"
     gene            155..1108
                     /locus_tag="VHV99_00740"
     CDS             155..1108
                     /locus_tag="VHV99_00740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3634022.1"
                     /translation="MPLREPFVKVSVIVPTYRRTADLARCLAALEAQERHADEVIVIA
                     RHDDYATLDWLRTREASRPDPRRWVVLVRQPGVVAAYNLGVDSASGDVLCFTDDDAAP
                     HPDWIARIARAFESDAALGGLGGRDIVHERNGILQGQKPRVGLVRWYGRTIGNHHIGH
                     GAAREVQVLKGVNMAFRREAIGTLRFDSRLRGTGAQVHCEMAFSLDVQRRGWTLIYDP
                     SLLVEHFPAQRSDEDQRFTFNDAAFYNASFNLRLIMCEYLTPAGRWAFVVYSTLIGDR
                     ADPGFLRALSLASERGGVALALRKWRVGLRAMRGAWQEAAR"
     gene            1146..2339
                     /locus_tag="VHV99_00745"
     CDS             1146..2339
                     /locus_tag="VHV99_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEX3634023.1"
                     /translation="MTMKTIAAGLAAASLCACSLAPGPYLDTDRLQPAAPPERTADKF
                     PVHAIDVGYFRQQRAAAVPAVCPLSCLTAQTRGAYDYRLGIGDQLSIIVWDHPELTGG
                     GAGAAVPPLPATSGTTPSPGPTQTQGATPIAPTLTGSGEGGLTVRVANNGTIFFPRVG
                     RIKVVGMNAQQVQEALSKGLSKTIRNPQLDVRVSGFNSQSVQVTGNLRTPASEAITDA
                     PLTVLDAINRAGGALPDADLQNVGVTRDGKRYTVDVAALLETGDPQQNVLLKDGDIID
                     VPDRANSRVFVLGEVNKPTSLPMNRGRLTLADALTGAGSLDVKTGDPRFVYVVRGADK
                     TLTPDVYQLDMTQVDALMLMTKFELQPKDVVYVQVSSAARFNRALEQISPTLQDLFYT
                     VQLSR"
     gene            2363..4741
                     /locus_tag="VHV99_00750"
     CDS             2363..4741
                     /locus_tag="VHV99_00750"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEX3634024.1"
                     /translation="MSTYDMLDHGPAPSSDEDAVMRDLLRMIMDQIWWVLGIAGCVIL
                     AAVIYTKLATPIYSADALLQVDPQSNTGGSQSQSAVSLMPSVGPMRTDAEIEIIKSRA
                     VVEPVVEQFKLNFSTAAKTIPVVGKISSWFARPGHPLGAAFGMNSYAWGGEQFEVGSI
                     TVPKSLEGARLTLHVLEYGRYELTDSFGQQVLTGVAGQEAHGNDVTLFVKTLVARPGT
                     EFYVTRFNQLDAVAALSSGLQVGEKGRDTGVVQLSYMGTDPHAITAITNAVAASYLAQ
                     RTERAQEEASHMLSFLNSELPRLRAEVQKTETALSEYQSKTGSFQPTQEAGVYLAGGL
                     DYEKQIATLRIQRAQLLQRFTEESPEVQQIDSQLAAMSREKARFEDHFSTLPSSERDA
                     LALQRDAKVAEEIYVTLLNKTQELSISRAGTIGNVHIIDQALLPSEPVRPKSALIISA
                     GTLLGIIAGVLFAFCRRTFFTGVADPEFVERRFQLPIFGSIAFSPEQARSDRQLSAMR
                     SAALPAPRSTPTEVPAGSSGGMRQLRSGSAVRQGGVPVGTQTVIASGKAPMRPLLVKT
                     HPYDSTVEGLRGLRATLQFGLIDAPNRIVAITSPAPSDGKSFLCANLAALIAESGKRV
                     LLIDADLRRGRLAQYLGRSPNGGLTELLTGQVDLEVAARATGVDGLHFIAAGAYPPNP
                     SEILTSSRFGEILARFEQEFDLVIVDTPPLLAVADAAVIAHIAGSTVLVMRSGAHTEG
                     HVAEALKKLRRARARVVGGVMNAVPLKTHNKNGTYDYAYAYTYSAGDPLDTH"
     gene            complement(5206..5628)
                     /locus_tag="VHV99_00755"
     CDS             complement(5206..5628)
                     /locus_tag="VHV99_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX3634025.1"
                     /translation="MNADRLPGAFPDIAQTWAALQTQLLITPIRNEQNYQTMVRLANS
                     LADHLNGNEEDPLADLFAIVTDLIESWEASHVTIPKAEPREVLRHLLETHGLKQKDLI
                     GIASPTVVSDILAGRRAISKKVAKALAVRFHTDVSAFL"
     gene            complement(5625..5939)
                     /locus_tag="VHV99_00760"
     CDS             complement(5625..5939)
                     /locus_tag="VHV99_00760"
                     /inference="COORDINATES: protein motif:HMM:NF021431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HEX3634026.1"
                     /translation="MRGNKMRVISKKALLDFIKRHPEARSALLTWYSLAQACLAYGYN
                     DLKQTFAGVDYVPPQYTVFDVGGNRFRIIAAIHCNRQSLFVRHVLTHAEYDHWTRKNL
                     NS"
     gene            complement(6182..7399)
                     /locus_tag="VHV99_00765"
     CDS             complement(6182..7399)
                     /locus_tag="VHV99_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX3634027.1"
                     /translation="MKVAIVHDWLVAPGGAEKVLEQIIECFPDADLFSLVDFLEDRRP
                     VGGKPVTTSFIQRLPFARRRYRAYLPLMPLAIEQFDLSGYDLVITSSYAVAKGVLVGP
                     DQTHVSYVHSPMRYAWDLQHQYLRDSNLTRGPKSWAVRALLHYLRGWDSHSANSVDRL
                     IANSQFVARRVMKTYRREAAVISPPVDVHKFELCTHKDDFYLTASRMVPYKRIDLIVE
                     TFTATPHRKLIVIGDGPEMAAIRAKAGPNVTILGYQPFKVLKDHLQRARAFVFAAEED
                     FGIVALEAQACGTPVIAFGKGGALETVVPIGEARPTGVYFARQTVVSMLDAIDRFERQ
                     GGQITAAACRANAERFSAAVFRRAFMAEVTRTIAASGWRLRVAAAERIEADLATEDLA
                     WPVERAQGSSWGR"
     gene            7615..8859
                     /locus_tag="VHV99_00770"
     CDS             7615..8859
                     /locus_tag="VHV99_00770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX3634028.1"
                     /translation="MPRVQPTLIDASAAPLSSISTATSATRSPTTPSVLFVDQSGQLG
                     GAEFALLQLAGSCTAQSDVVLLSDGPFRARLEALGARVQVLNDARVSGIVRRASGLNC
                     LRVVPGIVRQVRAIAARARAFDVLFLNTQKALVLGALGKPLHRKPVIWYQHDILTREH
                     FGRVQLSVIKWLVRFAVDHVVANSQASKQSLIALTGIAADSVPVIYNGIDAGAFQRID
                     ASDMAALRRRLGLPEHAWLAGLFGRLASWKGQHIALEAMARLPDVHLVLVGAPLFGED
                     VYAQGLHEQAERWGVADRVHFAGFQDDVPAWMKAMDVILHTSTEPEPFGRVIVEGMAA
                     ARPVIAAAAGGVPEIVRHRRNGWLVKPGDVAALAEAIGALRADPALARRLADQGRADA
                     QTEFSVEQYLQRMMQAIGQAAR"
     gene            complement(8932..9627)
                     /gene="rpiA"
                     /locus_tag="VHV99_00775"
     CDS             complement(8932..9627)
                     /gene="rpiA"
                     /locus_tag="VHV99_00775"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018418246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEX3634029.1"
                     /translation="MTQDELKQLVGQAAADYVNANVPEGSVIGVGTGSTANCFIDALA
                     ATRTRYRGAVSSSLATTARLQSHGFKVFDLNEIDSLPVYVDGADEIDHGGAMIKGGGG
                     ALTREKIVASVSDVFVCIADASKLVDTLGNFPLPIEVVPMARTAIGRRVTALGGVPVV
                     RVTREGVPFITDNGNEIIDVKGLRISDPRTLEMHINAWPGVVTVGLFAGRGANLCMLG
                     TDTGVETIEYSKS"
     gene            complement(9869..9949)
                     /locus_tag="VHV99_00780"
                     /pseudo
     CDS             complement(9869..9949)
                     /locus_tag="VHV99_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191786.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
     gene            10042..>10496
                     /locus_tag="VHV99_00785"
     CDS             10042..>10496
                     /locus_tag="VHV99_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX3634030.1"
                     /translation="MATFIKNTDKSGNVAWQVKIRRKGFPSQTKTFPTKALAQEWARF
                     IESSMDRAEHPTNRNATQTTLADILVRYRDTVVPTHRGAVHERQRTNALLKHAMSRCT
                     MAELTPGILGYWRDERLRTVSPGSVLREMTILRGAIKRARNEWGIDLRE"
BASE COUNT         1960 a         3358 c         3321 g         1857 t
ORIGIN      
        1 acggttatcg cggcgcgacc accaatgtcg aaaagatgga gacgcgcgtc aagttcgatc
       61 tcttctacat ccataactgg tcgttctggt tcgacatgaa gatcgtgttc atcacgatct
      121 tcaagggctt tgtcggccgc aacgcatttt gaccatgcca ctccgggaac ccttcgtgaa
      181 agtctccgtg atcgtgccga cttaccggcg caccgccgat ctcgcacgct gcctcgcggc
      241 actcgaggcg caggagcgcc acgccgacga agtgatcgtg atcgcacgcc acgacgatta
      301 cgcgacgctc gactggctgc gcacccgcga agcgagccgg cccgacccgc gccgctgggt
      361 cgtgctggtg cggcagccgg gcgtggtcgc ggcctacaac ctcggcgtgg acagcgcgag
      421 cggcgacgtg ctctgcttca ccgacgacga tgccgcgccg catcccgact ggatcgcgcg
      481 gatcgctcgc gcgttcgaaa gcgatgcggc gctcggcggc ctcggcggcc gcgacatcgt
      541 gcatgaacgc aatggcatcc tgcaggggca aaagccgcgg gtgggtctcg tgcgctggta
      601 tggccggacc atcggcaatc accatatcgg ccacggcgcc gcacgcgaag tgcaggtgct
      661 caagggcgtc aacatggcgt ttcgccgcga ggcgatcggc acgctgcgct tcgactcccg
      721 cttgcgcggc accggcgcgc aggttcactg cgagatggcc ttcagtctgg acgtgcaacg
      781 gcgcggctgg accctgattt acgacccgtc gctgctggtg gagcacttcc cggcccagcg
      841 cagcgacgaa gaccagcgtt tcacattcaa cgacgcggct ttctataacg cgtcattcaa
      901 tctccgactc atcatgtgcg aatacctgac gccagccggc cgatgggcgt tcgtcgtgta
      961 ctcgacgttg atcggcgatc gtgcggaccc ggggttcctg cgcgcattgt cattggcatc
     1021 cgaacgcggc ggcgtggcac ttgcgcttcg caaatggaga gtcggtttac gcgcaatgcg
     1081 cggggcatgg caggaggcgg cacgctgacc gatcagcgcg ccgcttcacg cgagggacca
     1141 ccaaaatgac tatgaaaaca attgccgcag ggctcgcggc cgcatctcta tgcgcctgtt
     1201 cgctggcgcc gggaccgtat ctggacaccg accggctgca gccggctgct ccgccggagc
     1261 ggacggccga taaatttccg gtccacgcga tcgacgtcgg ctattttcgc cagcagcgcg
     1321 cggccgcggt accggccgtg tgtccgttgt cgtgcctgac ggcgcagacg cgcggtgctt
     1381 acgattaccg gctcggcatt ggcgaccagt tgagcatcat tgtctgggat cacccggaac
     1441 tgaccggcgg cggcgcgggc gcggcggtgc cgccgctgcc cgccaccagc ggcaccacgc
     1501 cttcacccgg cccgacgcag acccagggcg cgacacccat cgcgccgacg ctgacgggca
     1561 gcggagaagg cggactgacc gtgcgggtcg ccaacaacgg cacgatcttc tttccgcgcg
     1621 tgggccgcat caaggtggtc ggcatgaacg cacaacaggt tcaggaagcc ctctcgaagg
     1681 ggctctcaaa gacgattcgt aatcctcagc tcgacgtgcg cgtgtcgggt ttcaacagcc
     1741 agtcggtgca ggtgaccggc aatctgcgca cgcccgcctc cgaagcgatc accgatgcgc
     1801 cgctgaccgt gctcgacgcg atcaatcgcg caggcggcgc attgcccgac gcggatctgc
     1861 agaacgtggg cgtgacccgc gacggcaagc gctacaccgt cgacgtcgcc gcgctgctcg
     1921 aaaccggcga tccgcagcag aacgtgctcc tgaaggacgg cgacatcatc gacgtgccgg
     1981 accgcgcgaa tagccgcgtg ttcgtactgg gcgaagtcaa caagccgacc tcgctgccga
     2041 tgaaccgcgg ccgcctgacg ctcgccgatg cgctgaccgg cgccggcagc ctcgacgtca
     2101 aaaccggcga cccgcgtttc gtctacgtgg tgcgcggcgc cgacaagacg ctcacgcccg
     2161 acgtctatca actcgacatg acccaggtcg acgcgctgat gctgatgacc aagttcgaac
     2221 ttcagcccaa ggacgtggtg tacgtgcagg tctcgagcgc agcacgcttt aaccgcgcac
     2281 tcgaacagat ctcgccgacc ctgcaggacc tgttctacac ggtccaactg tccagataaa
     2341 tcgcacgcaa cggagtatcc ctatgagtac gtacgacatg ctggaccacg gcccggcgcc
     2401 gagcagcgac gaagatgcgg taatgcgcga cttgctgcgc atgatcatgg atcagatctg
     2461 gtgggtgctc ggcattgccg gctgcgtgat tctcgccgcg gtgatctata cgaagctcgc
     2521 cacgccgatc tattccgccg acgccctgct tcaggtcgac ccgcagagca acaccggcgg
     2581 ctcgcaaagc cagagcgccg tatcgttgat gccctcggtg ggcccgatgc gcaccgacgc
     2641 ggaaatcgaa atcatcaaga gccgcgcggt ggtcgagccg gtggtcgagc agttcaagct
     2701 gaacttcagc acggctgcga aaaccatccc ggtggtcggc aagatctcct cgtggttcgc
     2761 gcgtccgggc catccgctcg gcgccgcctt cggcatgaac tcgtatgcgt ggggtggcga
     2821 gcaattcgag gtcgggtcga tcacggtgcc gaaatcgctg gaaggcgcgc gtctgacact
     2881 gcacgtgctc gaatacggcc gctatgaatt gaccgattcg ttcgggcagc aggttctcac
     2941 cggcgtggcg ggtcaggaag cccacggcaa cgacgtcacg ttgttcgtca agacattggt
     3001 ggcccgtccc ggcaccgagt tctacgtgac gcgcttcaac cagctggatg cggtcgcggc
     3061 gctttcgtcc ggcttgcagg tcggtgaaaa aggccgcgac accggcgtcg tgcaactgtc
     3121 gtacatgggc accgatccgc acgcgatcac cgcgatcacc aatgcggtgg cggcgtcgta
     3181 tctggcgcaa cgcaccgagc gcgcacagga agaagcgagc cacatgctga gcttcctgaa
     3241 cagtgaattg ccgcgtctgc gcgccgaagt gcaaaagacc gagaccgcgt tgtcggaata
     3301 tcagtcgaag accggttcgt tccagccgac ccaggaggcc ggggtttacc tcgcaggcgg
     3361 tctggactac gagaagcaga ttgcgacgct acgcatccag cgcgcgcaac tgctgcagcg
     3421 cttcacggaa gagagtcctg aagtgcagca gatcgactcg caactggcgg caatgtcgcg
     3481 cgaaaaagcc cgcttcgagg atcacttcag cacgctgccg agttcggaac gcgacgcctt
     3541 ggcattgcag cgcgatgcga aggtcgccga agaaatctat gtgacgttgc tgaacaaaac
     3601 ccaggagctg tcgatttcgc gtgccggcac gatcggcaac gtgcatatca tcgaccaggc
     3661 cttgttgccg tccgagccgg tacggcccaa gtcggcactg atcatttcgg cggggacgct
     3721 gctcggcatc atcgccggtg ttctgttcgc gttctgccgc cgtacgttct ttaccggcgt
     3781 ggccgatccc gagttcgtcg aacggcgctt tcagttgccg atcttcggct cgatcgcgtt
     3841 cagcccggaa caggcgcgca gcgaccgtca gttgagcgcc atgcgcagcg cggccctgcc
     3901 cgctcccagg tcgacgccga cggaggtgcc ggcgggctcg tcgggcggca tgcgccagct
     3961 tcgatcgggt tcggcggtcc gccagggcgg cgtgccggtc ggcacccaaa ccgtcattgc
     4021 gtcgggcaag gcgccgatgc ggccgctgct ggtgaagacc catccgtacg actcgacggt
     4081 ggaaggcctg cgtgggctgc gcgcgacgct gcaattcggc ttgatcgatg cgcccaaccg
     4141 catcgtggcg atcacgagcc cggcgccctc ggacgggaaa agcttcctgt gcgcgaacct
     4201 cgcggccttg attgcggaat cgggcaaacg cgtactgctg atcgacgccg atctgcggcg
     4261 cggccgcctc gcgcagtatc tcggccgctc gcccaatggc ggcctgaccg aactgctgac
     4321 gggtcaggtc gatctggaag tcgcggctcg cgcgacgggc gtggatggct tgcacttcat
     4381 cgcggcgggt gcttatccgc cgaatccgtc cgagattctg acgtcgtcgc gcttcggcga
     4441 aatccttgcg cgcttcgagc aggagttcga tctggtgatc gtcgatacgc cgccactgct
     4501 ggcggtggcc gatgcggcgg tgatcgcgca tatcgccggt tcgacggtgc tggtgatgcg
     4561 ctccggcgct cacacggaag gccacgtggc cgaagcgctg aagaagctgc ggcgcgcgcg
     4621 ggcgcgggtg gtgggcggtg tgatgaacgc ggtgccgctc aagacccaca acaagaatgg
     4681 cacgtatgac tacgcgtatg cgtataccta ctcggccggc gatccgctcg atactcacta
     4741 agcactgtag atcggcttaa aagcggttca tcgaaggggc tgcaccggac gaggtgcagc
     4801 ccctttttat atgctggcgg gcaggttcgt gcgaagggac gtcggcgcga ggttcatgcg
     4861 cgcggacgtt ggcgcggagt tcatgcgcaa ggacgtgtta gcgcgcggtt catgcgcaag
     4921 ggcgttggcg cgcggttcgt gcttaaaaac gttagcgcac gattcatgcg taaacacgtt
     4981 ggcgcgccgt tcatgcgcaa ggaacaggac tgggactgac gcgacaagcg ccaacgccga
     5041 tggcttggca tgcaagccgc cggcgtggcg tgaatggaac gaggaaaaag tgcagtacag
     5101 ctagcagtca gccgagtggc tgacgcttca tgtcacgctc tggcgaagct agtggcaaat
     5161 ggccaaaacc gaacgaccag cagaaggctg tgcgctgcgc ggttcttaca gaaacgcgct
     5221 gacgtcggta tgaaaacgca cggccagcgc cttcgccacc tttttgctaa tggcacgccg
     5281 gcccgcgaga atatcgctca ccacggtggg cgatgcgata ccaatcaggt ccttttgctt
     5341 gagcccgtgc gtctccagca gatgccgcaa cacttcgcgc ggctccgctt tcgggatggt
     5401 cacgtggctc gcttcccagc tttcgatcag gtcggtgaca atcgcgaaca gatcggccag
     5461 cggatcttcc tcgttgccat tgaggtgatc ggcgagcgag ttcgcgagcc gcaccatcgt
     5521 ctgatagttc tgctcgttgc gaataggcgt gatgagtagt tgggtttgca gcgcagccca
     5581 ggtttgggca atgtcgggaa aggctcccgg aaggcgatcg gcattcatga gtttaaattc
     5641 ttcctcgtcc aatggtcgta ttccgcatgc gtcaatacat gacgaacgaa caacgactgc
     5701 ctgttgcagt gtatcgccgc gatgatccgg aaacggttgc cgcctacatc gaaaacggtg
     5761 tattgcggcg gcacgtaatc cacgccggcg aacgtctgct tcaggtcgtt atagccatac
     5821 gcgaggcaag cctgcgccag tgagtaccac gtcaacaacg cactgcgcgc ctccggatgt
     5881 ctctttataa agtccagcaa cgccttcttt gaaatgaccc gcatcttatt tcctcgcatg
     5941 tacagacgcg ctcaacaagc acgctctacc tgcaaggcta atgggatggg atcgacgccg
     6001 atagtcggcc ggacggcata gaccgaacag ccagctaagg cttactgcgg tacgacaacg
     6061 tcattttatt cgcatattgc gaagttcgca acatgcgaat aaagagataa gcgatggcgc
     6121 ttcatggcgt cttcgcgaaa gcacgccact cactatcgcg ctgcacgcag acgcccacgc
     6181 atcagcgtcc ccagctgctt ccttgcgcgc gttcgacggg ccatgcaaga tcctcggtgg
     6241 ccagatcggc ttcgatccgc tccgccgccg ccacccgcaa acgccagccc gaggccgcga
     6301 tcgtgcgcgt cacttcggcc atgaaggcgc gccggaaaac cgccgccgaa aaccgttcgg
     6361 cgttcgcgcg gcatgccgcc gctgtgatct gtccaccctg ccgctcgaag cggtcaatcg
     6421 cgtcgagcat cgacacgacc gtctggcgtg cgaaatacac gccggtagga cgcgcctcgc
     6481 cgatcggcac caccgtttcg agcgccccgc ccttgccgaa agcgatgacc ggcgtgccgc
     6541 acgcctgcgc ttcgagcgcg acgatgccga agtcttcttc ggcagcgaat acaaaggcgc
     6601 gcgcgcgttg cagatgatcc ttgagaacct tgaatggctg atagccgagg atcgtcacgt
     6661 tcggacccgc cttggcgcga atcgccgcca tttccggacc gtcgccgatt acgatcagct
     6721 tgcggtgcgg cgtcgcggta aaggtttcga cgatcagatc gatgcgcttg tacggcacca
     6781 tgcgcgaggc ggtcagatag aagtcgtcct tgtgcgtgca cagttcgaac ttgtgcacgt
     6841 cgacgggagg cgaaatcacc gccgcctcgc gccgataagt tttcatcacg cgccgcgcga
     6901 cgaattgcga attcgcgatc agccggtcca cgctgttcgc cgagtgcgaa tcccagccgc
     6961 gcagataatg gagcaacgca cgcacggccc acgacttcgg cccgcgtgtc agattcgaat
     7021 cgcgcaaata ctggtgttgc agatcccacg cgtagcgcat cggcgaatgc acgtagctca
     7081 cgtgcgtctg atcggggccg accagcacgc ccttggcgac cgcataagaa ctggtgatga
     7141 cgaggtcgta accggagaga tcgaactgtt cgatcgcgag tggcatcaac ggcagatacg
     7201 cgcgatagcg gcgccgcgca aacggcaggc gttgaatgaa cgacgtcgtg acaggcttgc
     7261 cgcctaccgg ccggcggtct tcgaggaaat cgacaagact gaaaagatcg gcatcgggaa
     7321 agcactcgat gatctgttcg agcactttct cggcaccgcc tggtgcgacg agccagtcgt
     7381 gcacgatcgc cactttcatg atctccccgc ctattaataa acgcctcttg gccttagcaa
     7441 cgagtgtagg tgccggatca tttactgtgc gtgcgtatgc ccacattcga cgcgcgacag
     7501 tccgcgaata atgaagactc gccattcgcc tcttgcgaac ggcgcgttca atcactacgc
     7561 cggacatcgc actctatgcg cccaacgctc gacgcttctc ctctcagccg ttccttgcca
     7621 cgcgttcagc cgaccctgat cgatgcatcc gcggcgcccc tatcatccat ctcgacagcg
     7681 acctcggcaa cgcgctcgcc gacgacgccg agcgtgctgt tcgtcgacca gagcggccag
     7741 ctcggcggcg ctgaattcgc gcttctgcaa ctggccggaa gctgcacggc gcagagcgat
     7801 gtcgtgctgc tctcggacgg tccattccgc gcgcgcctgg aagcgctcgg cgcacgcgtg
     7861 caggtgctga acgacgcgcg cgtatcgggc atcgtccggc gagcgtccgg gttgaactgt
     7921 ctgcgcgtgg tgcccggcat cgtgcgccag gttcgcgcga tcgcggcgcg cgcgcgagcg
     7981 ttcgacgtgc tgtttctcaa cacgcaaaaa gcactcgtgc tgggcgcgct cggcaaaccg
     8041 ctgcatcgca aacccgtgat ctggtatcag cacgacatcc tcacacgcga gcatttcggt
     8101 cgcgtgcagt tgagcgtcat caagtggctg gtgcgcttcg ccgtcgatca cgtggtggcg
     8161 aattcgcagg cgtcgaagca atcgttgatc gcgctgaccg gcatcgcggc cgactcggtg
     8221 ccggtcatct acaacggtat cgacgccggc gcgttccagc gtatcgacgc cagcgacatg
     8281 gccgcgctgc gccggcgcct gggcttgccc gagcatgcgt ggctcgcggg tctgttcggc
     8341 cgcctcgcgt cgtggaaggg gcagcacatt gcgctcgagg ccatggcgcg gctgcccgac
     8401 gtgcacctgg tgctggtcgg tgcgccgcta ttcggcgagg acgtctatgc gcaaggtctg
     8461 cacgagcaag cggagagatg gggtgtcgcg gatcgcgtgc atttcgcggg ctttcaggac
     8521 gacgtgcccg cgtggatgaa agcgatggat gtgatcttgc atacgtcgac ggagccggag
     8581 ccgttcggtc gcgtgatcgt cgaaggcatg gcggcggcgc gcccggtgat cgccgcggcc
     8641 gcgggcggcg tgccggagat cgtgcggcat cggcgcaatg gctggctggt gaaaccgggg
     8701 gacgtggccg cgttggccga ggcgatcggc gccttgcgcg ccgacccggc gctggcgcgg
     8761 cgcctcgcgg atcaggggcg cgccgatgcg caaaccgagt tctcagtgga gcagtatttg
     8821 cagcggatga tgcaggcgat cgggcaagcg gcgcgctaaa gcgggttcac gtcaacgttg
     8881 caacgcaagc ccgcggaagc aggctcgcgt gcaactgcaa aagcgcggtg atcagctctt
     8941 gctgtactcg atcgtctcga cgcccgtatc cgtgccgagc atgcacagat tcgcgccgcg
     9001 gcccgcgaac aggcccaccg tcaccacgcc cggccacgca ttgatatgca tttccagcgt
     9061 gcgagggtcg ctgatgcgca aacccttgac gtcgatgatc tcgttaccgt tatccgtgat
     9121 gaacggcacc ccctccctgg tcacacgcac caccggcacg ccgccgagcg ccgtcacgcg
     9181 gcggccgatc gcggtacgcg ccatcggcac gacttcgatc ggcagcggga aattgccgag
     9241 cgtgtcgacc agcttgctcg catcggcgat gcagacgaac acgtccgaca ccgacgcgac
     9301 gattttctcg cgtgtcagcg ccccgccgcc gcccttgatc atcgcgccgc cgtggtcgat
     9361 ttcgtcggcg ccatccacat acaccggcag cgaatcgatt tcgttcagat cgaacacctt
     9421 gaaaccatgc gattgcagac gggcggtggt ggcgagcgag ctcgacacgg cgccccgata
     9481 gcgggtcctg gtggccgcca gcgcgtcgat gaaacagttc gcggtggagc cggtgccgac
     9541 gccgatcacg gagccttccg gcacgttggc gttcacatag tcggcggcgg cctggccgac
     9601 cagttgcttg agttcgtctt gggtcatggg gaatgcaggc gagcagtgaa aacgacaagt
     9661 ttaccggagt ggcgagggag cgctggttta ccggcgtggc gggtggcaac gctcgctcgc
     9721 catgggcgcc cgaatgcagt gcttgccggc cacatgcgag ccggccctgt cgagagcact
     9781 ttgagcgctc ggcaaacagc cgacgcgcgt ctcgcacggc gccttggctt cgaccggtgt
     9841 cgaacgcagc gccgcacgat gttgcaactt acttcttctt caccgaccgc tgacgcaccg
     9901 cttcaaacag acaaacaccg ctcgccaccg acacattcaa actctcgacc ggaaaggcgc
     9961 gtccacgctg aagcgcagcc gcttctaccc gcttgccatc gcagtgtttt tgatcccaca
    10021 ggacaattac aggacactga catggcaact ttcatcaaaa atacggacaa atcgggcaac
    10081 gtcgcctggc aggtgaaaat ccgccgcaag ggctttccct cgcagacgaa gaccttcccg
    10141 accaaggcgc tcgcacagga atgggcacgc ttcatcgaaa gctcgatgga ccgcgccgag
    10201 cacccgacga accgcaacgc cacgcagacc acgctggccg acattctagt ccgctatcgg
    10261 gataccgtcg tccctacgca cagaggagcg gtccacgagc gccaacggac aaacgccctc
    10321 ctcaagcatg ccatgagccg ctgcacgatg gcagaactta cgcccggtat cctcggctac
    10381 tggcgcgatg aacggctgcg caccgtgtca ccgggcagcg tgctgcggga aatgaccatc
    10441 ctgcgcggtg cgatcaaacg cgcccgcaac gagtggggca tcgacctgcg agagtg
//
LOCUS       DATASH010000026         4895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_992505,
            whole genome shotgun sequence.
ACCESSION   DATASH010000026 DATASH010000000
VERSION     DATASH010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4895
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992505"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..999)
                     /locus_tag="VHV99_00790"
     CDS             complement(130..999)
                     /locus_tag="VHV99_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEX3634031.1"
                     /translation="MNRQSRKIDKEAADSEPAGTEASAEEAFASYAAPLVSDAIEFAN
                     AVREDASPEALHKLRVSLRRLRSLWWAFEPLLDKAENTRHRALYKFLATAAGKTRDWD
                     ILIELIAHEEGVAREMAPKLQEARSDALATSRETLSNADVKHLLREALSGAAKELNTA
                     HERVPLRKFAEKRVSDSERSLKKRMKRASRAKRSNYPSFHDVRKAGKKLRYLLEFFEP
                     VLKGGRKRALKRLKQIQKRFGALNDIVASEMLLRANMALLSGSGDVDAVLLWFRKERK
                     RRMRSAAGLLRKL"
     gene            1415..3001
                     /locus_tag="VHV99_00795"
     CDS             1415..3001
                     /locus_tag="VHV99_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEX3634032.1"
                     /translation="MPELSYPQPGTANGKGRNLGLIVFLVVIAIGAAYCAMHLVDDLQ
                     SVRQASYLPYLLLGIALLIALGFEFVNGFHDTANAVATVIYTHSLAPNIAVVWSGGWN
                     FLGVLTSSGAVAFGVLQLLPVELILQVGSSAGFAMVFALLIAAIIWNLGTWYFGLPSS
                     SSHTLIGSIIGVGLMNQLMHGSNGTSGVDWNQALGVGKSLLFSPLVGFLAAGLLLLIL
                     KAVVRIPALYAEPKGKEPPPFWIRCLLILTCTGVSFAHGSNDGQKGMGLIMLILIGTV
                     PTAYALNKAVTPAETQTFIAVAQQASATLAKYTQGATPSANPRADVETYVRTRQLTPA
                     TLPALQQLTNQISGQVSASGSMAAVPQSIVDNVRNNMYVASEAIRLMEKNKQPAFSPE
                     DIKVIHNFKAQTDHATKFIPTWVKVAVAIALGLGTMVGWKRIVVTVGEKIGKQHLTYG
                     QGASAELVAMLTIGAADMYGLPVSTTHVLSSGVAGTMAANGSGLQWGTVRSLVLAWVL
                     TLPVSIALAGGLYWVFRMVF"
     gene            complement(3128..4054)
                     /gene="ppk2"
                     /locus_tag="VHV99_00800"
     CDS             complement(3128..4054)
                     /gene="ppk2"
                     /locus_tag="VHV99_00800"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HEX3634033.1"
                     /translation="MKELDPRPEAEVMAERQRRFEEDLIDAYDEELEMEVDDRIIDGA
                     EGFTPEHREARKMYFRELFRLQGELVKLQDWIVQTGHRLVVIFEGRDAAGKGGAIKRI
                     TQRLNPRVCRVAALPAPNNRERTQWYFQRYVSHLPAGGEMVLFDRSWYNRAGVERVMN
                     FCSDDEYEEFFRSVPEFEKMLARSGVQILKYWFSITDEEQEIRFQNRIHDPLKQWKLS
                     PMDLESRRRWEAYTQAKEVMLQRSHIPEAPWWVVQAVDKKRARLNCIHHLLSQVPYHE
                     IEHSQIELPARVYNDEYSRQPVPASMIVPEVY"
     gene            complement(4249..4719)
                     /locus_tag="VHV99_00805"
     CDS             complement(4249..4719)
                     /locus_tag="VHV99_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3634034.1"
                     /translation="MNSPSSTASSASSSASSASGASSASATSSAPADSTKPLEPRIES
                     LSAITLATRDMPRAVLFYEALGFPLKFGGSHEAFTSFAFGGSYLNLIVDTRAPVNWWG
                     RVIIYVSDVDALYRKALAAGLKPSLEPSDAPWCERYFHITDPDGHELSFARPLR"
BASE COUNT          879 a         1532 c         1450 g         1034 t
ORIGIN      
        1 acaggtccag cccgccgacc gccagcacga gtcaaacgga tccgccgcgc ccacaaccac
       61 gaatcccgcg agatcgccca tgtcgtgatt cggcccgggt tactcgaacg agccgcaccg
      121 cgccgcgcat cacagtttgc gcagcaatcc agccgccgac cgcatccgcc gtttgcgttc
      181 ctttctgaac cagagaagca ccgcatcgac gtcgccagac ccggacagca gagccatgtt
      241 ggcgcgtagc agcatttcgc ttgcgacaat atcgttcagc gctccgaaac gtttctgaat
      301 ctgcttcaaa cgcttcaggg cacgtttgcg gccccccttc agaacgggct cgaaaaactc
      361 gagcaggtag cgcagtttct tccccgcctt tctaacgtcg tgaaacgacg gatagttgga
      421 acgtttcgcg cgggatgcgc gtttcatacg cttcttcaac gaccgctccg agtcgctcac
      481 gcgcttttcg gcaaattttc gcagcggcac gcgctcatgc gccgtgttca actccttcgc
      541 agcgccgctc aaggcctccc gcagcagatg tttgacgtcc gcgttcgaca gcgtttcgcg
      601 gctcgtcgcc aacgcatcgc tgcgagcctc ctggagtttc ggcgccatct cgcgagcaac
      661 accctcttcg tgtgcaatca gttcgatcag aatgtcccag tcgcgcgtct taccggccgc
      721 cgttgccaga aacttgtaca acgcgcggtg gcgcgtgttc tccgctttgt cgagcaaggg
      781 ttcgaacgcc caccacaagg agcgcaagcg ccgcagcgat acgcgcagtt tgtgcaacgc
      841 ttccggcgag gcatcttcgc gcacggcgtt agcgaattcg atcgcgtcac tgacgagcgg
      901 cgccgcgtac gacgcgaagg cttcttcggc cgaggcctct gtgccggccg gctcgctgtc
      961 cgcagcctct ttgtcgattt tccggctctg tcggttcatt ggacgccttt caaaagtccc
     1021 tttatgtcag gctagcaaaa aatggtcccc gtggatatcc gtcgaaacgt cgcctaaact
     1081 gcacgaggat gaccgcagga accatgcgtc gcatggaccg cactccacca caaattgtgg
     1141 tcgtctccag aatcgcccgg aatctgcatg cgcccttgac ggaagtttga accggaaaat
     1201 ccactcactc agtacggatg cttcacacgt agttattttc ggacagctta tcattatgag
     1261 ccgccgcgca ggcggtggcc tcgctcgtag cgtcaatatc cacgcacctt tccgcgaaaa
     1321 agccgtccgg cctgagcgtc acgccactgt catcattacg acatactggc cccgcaggat
     1381 cggtagttcc gcgacaaact ttggggctca cgccatgccg gaactttcgt acccacaacc
     1441 gggcactgca aatggaaagg ggcgcaatct cggcctcatc gttttcctcg tcgtgattgc
     1501 gatcggcgcc gcgtattgcg ccatgcatct ggtggacgat ttgcaatccg tccgccaagc
     1561 ctcgtatttg ccttatctgc tgctgggcat cgcgctcctg atcgcgctcg gcttcgaatt
     1621 cgtgaacggt tttcatgaca cggcgaacgc agtcgccact gtgatctaca cgcattcgct
     1681 cgcgccgaac atcgcggtgg tctggtccgg cggttggaat ttcctcggcg tgctgacttc
     1741 atctggcgcg gtggcattcg gcgttctgca gttgctgccc gtcgaactga ttctgcaagt
     1801 gggcagcagt gccggtttcg cgatggtctt cgccctgctg atcgcggcga tcatctggaa
     1861 tctgggtacc tggtatttcg gactgccatc gtcgagttcg cacacgctga ttggctcgat
     1921 catcggcgtc ggcctgatga atcagctgat gcacggctca aacggcacga gcggcgtgga
     1981 ctggaatcag gcgctcggcg tgggcaagtc gctgctgttt tcgccgctgg tcggcttcct
     2041 cgccgccggt ttgctgctgc tgatcctgaa ggccgtggtg cggattcccg cgctgtacgc
     2101 cgagccgaag ggcaaggaac cgccgccgtt ctggattcgc tgcctgctga ttctgacctg
     2161 tacgggcgtt tcattcgcgc acggttcgaa cgacgggcag aaaggcatgg gcctcatcat
     2221 gctgatcctg atcggcacgg tgccgacggc ctacgcgctg aacaaggcgg tcacgccggc
     2281 ggaaacgcag accttcatcg ctgtcgcgca gcaggcctcg gccacgctgg ccaaatacac
     2341 ccagggcgcg acgccgtccg ccaatccacg cgccgacgtc gaaacctatg tacgcacccg
     2401 ccagttgacg cctgccacac tgcccgcctt gcagcaattg accaaccaga tcagcggcca
     2461 ggtcagcgcg tccgggtcga tggcagccgt gccgcaaagc atcgtcgata acgtgcgcaa
     2521 caacatgtat gtggcgtccg aggcgatccg ccttatggaa aagaacaaac agccggcctt
     2581 ctcgccggaa gacatcaagg tgatccacaa cttcaaggcg caaaccgatc acgccaccaa
     2641 gttcatcccg acgtgggtca aggtcgcggt ggccatcgcc ctgggtctcg gcacgatggt
     2701 cggatggaag cgcatcgtcg tcacggtcgg cgagaaaatc ggcaagcagc atctgaccta
     2761 tggacagggc gcatcggccg aactggtggc gatgctgacg atcggcgcag ccgatatgta
     2821 cggtttgccg gtctcgacca cgcatgtgct gtcgtcgggc gtggccggca ccatggcggc
     2881 caacggctcc ggcttgcaat ggggcaccgt gcgcagcctg gtcctcgcct gggtgctgac
     2941 gttgccggtc tcgatcgcgc tggcgggagg cttgtactgg gtgttccgga tggtgttctg
     3001 aaaccagtgg cgccgctgat aggatcggcg cgccgcttaa aaccgttggt ggccgattgc
     3061 caatcggcct tgaaggaaaa aagcgcggtc tgcaccgcgc tttttcgttt caacgagccg
     3121 aaaccgatca atacacttcg ggcacgatca tcgaggccgg caccggctgg cggctgtatt
     3181 cgtcgttata aacccgtgct ggcaattcga tttgcgaatg ctcgatctcg tgatacggca
     3241 cctggcttag cagatgatga atgcagttca ggcgcgcgcg ttttttgtcg actgcctgca
     3301 cgacccacca cggcgcctcg ggaatgtgcg agcgttgcag catgacttct ttcgcctgcg
     3361 tataggcttc ccagcggcgc cggctttcca gatccatcgg actcagcttc cactgcttca
     3421 gcggatcgtg gatgcggttc tgaaagcgga tttcctgttc ttcgtcggtg atcgaaaacc
     3481 agtacttgag aatctgcacg ccgcttcgcg ccagcatctt ttcgaattcg ggcaccgagc
     3541 ggaagaactc ttcgtactcg tcgtcgctac agaaattcat cacgcgttcg acacccgcgc
     3601 ggttgtacca gctgcgatcg aacagcacca tttctccgcc ggccggcaaa tgcgacacgt
     3661 aacgctggaa ataccactgc gtgcgctcgc gattgttcgg tgcgggcaat gccgccacgc
     3721 ggcacacgcg cggattgagc cgctgcgtga tgcgtttgat cgcgccgccc ttgccggccg
     3781 cgtcgcgtcc ctcaaaaatc accaccagcc gatggcccgt ctgcacgatc cagtcctgca
     3841 gcttgacgag ctcgccctgc aaccggaaca gctcgcggaa gtacatcttg cgtgcttcgc
     3901 gatgctcggg cgtaaagccc tcagcgccgt cgatgatgcg gtcgtcgacc tccatttcca
     3961 gttcctcgtc gtacgcgtcg atgaggtctt cctcgaaacg gcgttgccgt tccgccatga
     4021 cttcggcctc gggtctcgga tccagctctt tcattgcgac tctcctggca acggaaatgg
     4081 ttaggcgcgc ggcgctgcgc gtcgcgaatg cgttcgatta tcgaagcgcg aagtgtcagc
     4141 cgtgttactg gcgcgtcagc cgtgccttct tgggccgatg gacgctcggc ggtggcctgc
     4201 gtgccttttg aacatccatg atagcgaatt tagcgcggtc gccaggcgct accgcaacgg
     4261 cctcgcgaag ctgagttcgt ggccgtcggg gtcggtaatg tggaaatagc gctcgcacca
     4321 cggcgcatcg gacggttcga gcgacggctt caaacccgcc gccagcgctt tccgatagag
     4381 cgcgtcgaca tccgacacgt agatgatgac gcgcccccac cagttgaccg gcgcgcgcgt
     4441 atcgacaatc aggttcagat acgagccgcc gaatgcaaac gacgtgaatg cttcgtgcga
     4501 accgccgaat ttgagcggaa aaccaagcgc ttcgtaaaac agcaccgcgc gcggcatatc
     4561 gcgggtggcg agcgtgatgg cgctgagcga ctcgatgcgc ggctcgagcg gtttcgttga
     4621 atccgcgggg gcgcttgaag tggctgaagc gcttgacgca cccgacgcgc tcgacgcgct
     4681 cgacgaggca gaggatgcag ttgacgaggg cgagttcatg gcggctgtct ccttgtgggc
     4741 attgcggggc ctgaaaggcc tgcatgacgt gtgcccgggt tctgctggtt ctgctggttc
     4801 tgctggttct gctggttctg ctggttctgc tggttctgct ggttctgctg gttctgctgg
     4861 ttctgctggt tgcgctggtt ctgctgattc cactg
//
LOCUS       DATASH010000027         4310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4553417,
            whole genome shotgun sequence.
ACCESSION   DATASH010000027 DATASH010000000
VERSION     DATASH010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4553417"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="VHV99_00810"
     CDS             complement(<1..549)
                     /locus_tag="VHV99_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634035.1"
                     /translation="MKQMRDESVRNGSRRTTACIGALWSKAGARCVLAGAAGISLALA
                     LAACGATSAGDTASGTQEAPAAQEAVAAAASSASSGNVSLQTALKMNMTSLSPDASSD
                     RFIVKYKTGTAERGAASAVQSKLDRLANAFPSKARHMRRMGTGADVLTTERKLTAKET
                     RAFMRAIASDPNVEYVEPDTEMS"
     gene            complement(1064..2302)
                     /locus_tag="VHV99_00815"
     CDS             complement(1064..2302)
                     /locus_tag="VHV99_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018434383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter"
                     /protein_id="HEX3634036.1"
                     /translation="MNSHSSASPTQAQANDASRALTWRDALWLCAIVVIGVNLRPLLT
                     SISPLMTTIRDATGLSFYGASLLTSLPVVAMGIGAFGAGALSRSIGETRGIALGLLAI
                     ALACGVRWSTASGAALLATAVLAGVGVAAIQALLPAVMKQRFPARVPLAMGVFSASIM
                     GGGGLGARLSPWVSAAMGSWHAGLAVWALPAVAALLGWLGLNRRSLHGPAAQPVAADA
                     MAFSLWRKRRAWTLGLYFGIVNGGYTTLVAWLPAFYQQHGASVADSGSLLAGMTVFQA
                     AAALLLPLAAAPFRDRRPWLVVGLSAQLLGLLGLIFWPDTAPLVWVGVAGAGLGGTFS
                     LTLVTAMDHSAHHTVAGKLVAFTQGVGFVVAAIAPVFAGMVRSWTGGFGAAWVMLAVS
                     IAAMIMVSLFFSPRSYAKWH"
     gene            2563..3306
                     /locus_tag="VHV99_00820"
     CDS             2563..3306
                     /locus_tag="VHV99_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX3634037.1"
                     /translation="MASRSSRTPSADSGTPIRSAPDDDPISVEERIYASITAALLQGR
                     LRPGAQLVERDLAAAFGCTRGALRKVLARLGFEGKLVLEANRGAFVPSPSEEGIRQVY
                     RARQIVEAGIVAALCGSLSAAHKRRLRAHVRSEEKASRAGALEESVRLAGRFHVLLTE
                     LAGGTELLGLVGQLVAKTELYKALFDPSKGSTCSADEHMQIIEALDAGDLHAALAAMR
                     EHLSELEERVVAQVRGRAAEEDIGAVFGV"
     gene            complement(3492..3764)
                     /gene="infA"
                     /locus_tag="VHV99_00825"
     CDS             complement(3492..3764)
                     /gene="infA"
                     /locus_tag="VHV99_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEX3634038.1"
                     /translation="MAKEELLELDGIVDEVLPDSRYRVTLDNGVVVGAYASGRMRKNH
                     IRILAGDRVTLELSVYDLTKGRINFRHKDERATGGGGARNTQFRRR"
     gene            complement(3901..4182)
                     /locus_tag="VHV99_00830"
     CDS             complement(3901..4182)
                     /locus_tag="VHV99_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634039.1"
                     /translation="MNSVVKTYKGYEIHPLVYPRRPADGQTSRNPDAGYDASVRICRV
                     GANPAADGRVFRLQYLFPFDGTGKARIACMAHAEQLIDGRVDGQSVADL"
BASE COUNT          855 a         1321 c         1380 g          754 t
ORIGIN      
        1 cgacatttcc gtatcgggtt cgacatattc gacgttaggg tccgatgcga tagcgcgcat
       61 gaatgccctg gtctccttcg ccgtcagctt gcgttccgtt gtcaacacgt cggcgccggt
      121 gcccatgcgg cgcatgtggc gtgccttcga cgggaaggca ttggccagcc ggtcaagctt
      181 tgactggacc gccgatgccg ctccgcgctc ggcggtgccg gttttgtact tgacaataaa
      241 gcggtcgctg gacgcatctg gcgacaggct ggtcatattc atcttcagcg ccgtttgcag
      301 cgaaacattg cctgaactgg cgctgcttgc cgccgccgcg actgcctctt gagctgcggg
      361 agcctcttga gttccggaag ccgtatcgcc ggcgctggtc gcgccgcacg ccgcgagtgc
      421 cagcgcaagc gatataccgg cggcgcccgc taacacacag cgcgcgcctg ctttggacca
      481 cagcgcgccg atgcaggccg ttgttctacg agagccgttc cgcacgctct catctctcat
      541 ctgtttcacg tttattcctg gtacgacata cagaaatgat tgcccctgtt tgatgaagta
      601 gtcgtactgc ggtcatcagc ggtgctacag ccctgggcca ccatctggtc ggacggtgcg
      661 gactgtttcc aggcggggtt aagattccta actaatgacc gttggtgacg gctgcgtggt
      721 acatgacctt ctgcggctgt tagtcagcca gtcaggttct tgtgtttgga cgggtacaga
      781 aaatagcgtg aacaggagaa taaatagcct gattgatata agcaatcagg ggtttctatt
      841 tgtccgtcag ataacttact tgtcgcgtaa ggctattggc atgtgccctc gccagcctac
      901 gaatcaaatc agtctggcga ctaaggtggg cagggggaag ggccgaggga atcgcgtgga
      961 attgcgcgta ttgcgcactg ggtggcacgc gttgcgtgag gccaggcgag ggcttgctcg
     1021 ctgccgcgtc agcgcaccag cacgagggca aagtccgccc gaatcaatgc catttcgcgt
     1081 aactgcgtgg cgagaagaag agactcacca tgatcatggc ggcaatgcta acggccagca
     1141 tgacccaggc ggccccgaac ccgcctgtcc agctccggac catgccggca aaaacgggcg
     1201 caatggccgc aacaacaaag ccgacgcctt gtgtaaacgc gacgagtttt ccggccacgg
     1261 tgtggtgcgc cgaatggtcc atggcagtca caagcgtcag ggagaaagtg ccgccaagcc
     1321 cggcgccggc aacgccaacc cacaccaacg gcgcggtatc gggccagaag atcaagccaa
     1381 gcaagccgag cagctgcgcc gacaatccga cgactaacca ggggcgccga tcgcgaaacg
     1441 gtgcggcggc aagcggcagc aataaggccg ccgcggcctg aaacaccgtc atgccggcaa
     1501 gcagcgaacc gctatcggcg acactcgcgc catgctgttg atagaaagcc ggcaaccacg
     1561 cgaccagcgt cgtatagccg ccgttcacga tgccgaagta caagccaagc gtccacgcgc
     1621 gccgcttgcg ccaaagcgaa aaagccatcg cgtctgccgc aacgggctgt gccgcggggc
     1681 catgcagaga tcggcggttc aaacccagcc agcccagcaa cgccgcgaca gcggggagtg
     1741 cccagaccgc gagccccgca tgccacgacc ccatcgctgc gctcacccac ggactcaagc
     1801 gcgccccgag gccgccgcct cccatgatcg atgccgagaa aacccccatc gccagcggca
     1861 cgcgtgcagg gaagcgctgc ttcatgacgg cgggcaacag cgcctggatc gccgcgacac
     1921 cgacgcccgc gagcacagcc gtcgcaagga gcgctgcgcc gctggcggtc gaccaacgga
     1981 cgccgcaggc cagcgcgatc gccagcaagc cgagcgcaat gccgcgcgtt tcaccgattg
     2041 accggcttag cgcgccggcg ccgaatgcac cgatgcccat cgcgacgacc ggcaggctgg
     2101 tcagcaacga cgcgccgtaa aagctcagcc cggtagcgtc gcggatcgtc gtcatcaagg
     2161 ggctgatcga tgtcagcagg ggccgcagat tgacaccgat caccacaatc gcgcacagcc
     2221 ataacgcgtc gcgccacgtg agcgcgcgcg atgcgtcgtt ggcttgggct tgggtcgggc
     2281 tggcgctgga gtgcgaattc acagtggtcc tgaatgcggg agtgggcgac gccttgccag
     2341 cggaagaagg acgctgcgcg accggggcgg caaaacgggt aacgaggctc ggacagaatg
     2401 tgaaaaagac tgttccaacc cattttggtc aacaataata accttaaatg gttaaccatt
     2461 tttctgattc ccggtataag tcctggcgcc cgcggatggc gtccaccacc tcgcatcgct
     2521 acaatgccgg gcaatatctc cctgttctcc gtcactgaat tcatggcatc ccgttcctcg
     2581 cgtacgccgt ccgccgacag cggcaccccc atccgcagcg cgcccgacga cgacccgatc
     2641 agcgtcgagg agcgcattta tgcgtcgatc accgcggcgt tgttgcaagg ccgcttgcgg
     2701 ccgggcgcgc aactggtgga gcgcgatctc gccgctgcgt tcggctgcac acgcggcgca
     2761 ttgcgcaaag tgctggcgcg gctgggcttc gagggcaagc tggtgctcga ggcgaatcgc
     2821 ggcgcgtttg tgccgtcgcc atcggaagaa ggcattcgcc aggtgtatcg cgcccggcag
     2881 attgtcgagg cgggcatcgt ggcggcttta tgcgggagtt tgagtgcggc gcacaagcgg
     2941 cgcctgcgcg ctcatgtgcg cagcgaagag aaggcgtcgc gcgccggcgc gctcgaggaa
     3001 tcggttcggc ttgctggccg gtttcatgtg ttgttgacgg aactggcagg cggcacggaa
     3061 ttgcttggcc tcgttgggca acttgtcgcg aagaccgagt tgtacaaggc gttattcgat
     3121 ccgtcgaagg gttcgacctg ctccgcggac gagcacatgc agatcatcga agcgctcgat
     3181 gccggggatc tgcatgcggc actggctgcg atgcgcgagc atctgtcgga gttggaggag
     3241 cgggtggtcg cgcaggtgcg cgggcgtgcc gccgaagagg atatcggtgc ggtgttcggg
     3301 gtgtaaagaa gagcagcagc gaagagccgc ggcgcgccgc tcaggctcga gtcaacgaat
     3361 ccagccggag gaggcgcgcg aaaggcttcg cgaacaacaa acccgcaatg ggccacgcaa
     3421 gaaccgatca aaagtcagcc agacgagcag ctggatcggc aactcaatga acggtcagcg
     3481 tgccgccagc ctcaacggcg acggaattgc gtattgcgtg cgccgccgcc accggtggcg
     3541 cgctcgtctt tgtgacggaa attgatccgg cctttggtca gatcgtaaac cgacagttcc
     3601 agcgttacgc ggtcgcccgc gagaattcgg atatggttct tgcgcatgcg tccggacgcg
     3661 taagcgccaa ccacgacgcc gttgtcgagc gtcacgcggt aacggctatc cggaagtact
     3721 tcgtcgacga taccgtcaag ttcaagcagt tcttctttcg ccatgcataa ctcctggtcg
     3781 atgggataag ggggtgttga gtcgttcggc gaggccgccc ggcacaacat ctgatacggt
     3841 gggccgctgg ctttcaggcc agctgcgttg cggtaacagc cgattcgctg atagtaccgc
     3901 tcaaagatcg gcaacggact ggccgtctac gcgaccgtcg atcaattgct cggcgtgggc
     3961 catgcacgca atgcgcgcct tgcccgtgcc gtcgaacgga aacagatact gcagacggaa
     4021 cacacgcccg tcggctgccg gattggcgcc cacccggcag atgcgcaccg acgcgtcgta
     4081 ccctgcgtcc ggattgcggc tcgtctgacc gtccgccggg cggcgcggat agacgagcgg
     4141 gtgaatttca taacccttgt acgtcttgac aactgaattc atgaagcacg tgtcctgtat
     4201 tacgtggcta gcatgtcacg cgtgttacgc gcggcaagac aggccgggca gcgcagcacc
     4261 ctggggcgcg ccgccattga aaaaggttgg cacaggcccg atggcaaggg
//
LOCUS       DATASH010000028         9770 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_597278,
            whole genome shotgun sequence.
ACCESSION   DATASH010000028 DATASH010000000
VERSION     DATASH010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 9770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9770
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597278"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            250..696
                     /locus_tag="VHV99_00835"
     CDS             250..696
                     /locus_tag="VHV99_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634040.1"
                     /translation="MSAESFAKFCTRDDLPGFDFIGMHGIWSWISDENRSLIADFIRG
                     KLNDGGVVYMSYNTQPGWATMLPVRELMHCHFQASARLNAHDGVPTETQVQMHIAAAL
                     DFVKTVIATQPGYAVVNPLLPERVDALGNENPHYLAHEYSIATGSP"
     gene            693..1535
                     /locus_tag="VHV99_00840"
     CDS             693..1535
                     /locus_tag="VHV99_00840"
                     /inference="COORDINATES: protein motif:HMM:NF021624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
                     /protein_id="HEX3634041.1"
                     /translation="MTFSKATSSLAASGLTYGCSADYRDHVDEINLNPAQRALLAGIP
                     DTALRETVRDFCINRSMRRDYWVKGSQALSEEAQQTALRTHRVMLALPRDLVLLKVRG
                     ALGEATLPERLYGPILDGLASRHPACLADIENSVRGREITLALIVKAVTFLVGAGVLP
                     NVQDEAHIELARPSSNRLNAAICEKALQHGDVQFLVSPVSGSGVLIPRAPQLFLLARR
                     RHLSEPAKWAEFAEAALRASPAPALVAEGASVSPLNTSGDLIVKANRFTEIHLPILQA
                     LGIA"
     gene            complement(1728..2843)
                     /locus_tag="VHV99_00845"
     CDS             complement(1728..2843)
                     /locus_tag="VHV99_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015600131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634042.1"
                     /translation="MKMKIFLSAISAAVLLSACGGSNNVTATPGVAPANTAQVLNTAT
                     AAGYSLSVFAKAPSANAKPDSIVQYNNTIFIGYQMAGDVKDGSVPGLTNTVVQYDLSG
                     NVLKSYTVPGHVDGLMARTDTNTLWAMSNEDGNPELTIIGLATGAQKTYQATVNPTEH
                     GGGFDDMQLMNGVVYVSASAPSTPGAAPTVVSLMLNPNGTTFDVAPVLAGNASMIDIT
                     PSVGGSANPTFNQPITFNLTDPDSEAIDAAGDLVLDSQADGKLVFIHNPGASQSVSIL
                     SLTLYNDKDGPTYPVDDTRWVPAPGAKGTTFMLFTDASNTTYRVDAPFKQGDAYSAGQ
                     GQVMQLDVKTGHLTPVVAGIGSASALQDPHGVLFVAL"
     gene            complement(3497..3835)
                     /locus_tag="VHV99_00850"
                     /pseudo
     CDS             complement(3497..3835)
                     /locus_tag="VHV99_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012732717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            4310..7114
                     /locus_tag="VHV99_00855"
     CDS             4310..7114
                     /locus_tag="VHV99_00855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX3634043.1"
                     /translation="MPNRHMTGLLVGLVSGATMSGAYAADTATLQGHVLSSANKRPLA
                     GARIQIRETGTSTTTSSDGAFSFTSLAAGQYTLVITPTRAAAVERTVVVRENDTTSDD
                     LTVDAAVPSLDRVTVTSQRTANVVARATQQSAANMVNILTADDIRKMPDVSAAEAVAR
                     APGVSLETDSGQGRYVNIRGLDTNLNSTTFGGIHLPPTNPASAQNGGRAVALDTIPAG
                     MIGQIVVTDTNKPEQDADALGGTIDISPRKLPPSGKPFLDARLGSGVETLRGSTIQDV
                     EMTAGARFGAHGIAGLGSGITSYSDAPFTFIGTFAYDQDHRGVDDEENAYIDATGVPN
                     KAYAQTDPRYYNYHRKRLGYGGELDFQPNADNKWYFRYYNFGYTENVNRQDIQTNFTG
                     NPVQNADGTLTDTATYAKGLRDEKETLRTQLFSLGGENNIGPGKIDYRAGFTSGTYDK
                     PYDLNSTFNNGAPATVTYFPGPDFATQSIVSGPNPADPAGYTLGQIVNSSQYVKTNEW
                     SANVNYTLPTHFTSAEDEQFKFGAGLRRMTFASAQSNYTYSPASGIPLSQAVFGAPNV
                     FYNGMYNVGPSISDGVMSGIYGAAAANGNLTAYPLKDAAAFSAINENIYSAYGQYQFG
                     WGRLGVLAGLRGEWTQDAFSGNLINGSSVTPNQTSNNYFNLFPSLQLRYDFTPTLVGR
                     AIYSSTIARPGFAQSSASVMADPGTGTVTTGNPNLKPTTSQNFDIQFEKYLPHGGIIS
                     LGAFDKSMTDYVVGQSSTVFSSPYLPGYSGPIRLVSFANVSSARVRGVTANYEQHFTQ
                     LPGFLSGLGAGANWTWTNSHLDIRPGDDHTLPSGPRNSYNASVFYERGPLDMRLSASY
                     VGKSLWAVGSSAATDVYTASRFSLDFGGSYQIRKDVGIYLDVRNILNTPLKFYEGSPD
                     RPIQREFYGPTVMVGLTINE"
     gene            7136..8212
                     /locus_tag="VHV99_00860"
     CDS             7136..8212
                     /locus_tag="VHV99_00860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634044.1"
                     /translation="MKLKSIVQAVTTLALSSALLLGSTAHAVPGANHGEFEVAKHYAL
                     GGTEGWDYVGYDAVRQHLFISRDSHVQVVDTGSGKLVGEVANTDGVHGFAFVQDQKLG
                     FITNGRADTITVVDLDTLRTVGTMKAGGPDPDGIMYVPQLKRLYVSNGHDQSVSAIDP
                     VTRKVVATMPVGGKPESLAADSDGRIFVNVEDKGEIVAMDGKSNKVLAHWSIAPCEGP
                     SGLAIDTKSERLFTVCANNRMMVVDARSGRQVAQLPIGGHPDAAAFDPVLKTAFSSNG
                     ADGTLTVVHEDDADHYTVLQNVPTEVGAKSMVLDGDAHRVYLVSSKFGSTPAATADNP
                     HPHPSIVPGTFNVLVAQRMQQSSQ"
     gene            8308..8919
                     /locus_tag="VHV99_00865"
     CDS             8308..8919
                     /locus_tag="VHV99_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX3634045.1"
                     /translation="MLRIIIDSSVQSFGLLPLMAVIFVAALAIIIERAIFFGTSVRSG
                     VSLEYDLKQVGRAKADDVKKLVQHYQGSVQSELMRSAIESQGQPVEDFEREVEESIMF
                     QMRKLDRNLWVLDTAVTLGPLLGLLGTIIGMIGSFNILGSSGTSDPMAVSGGIAHALI
                     ATACGLTIALVGVVALNYLNKRVRMVLLQMDIIKSMLVTRLAA"
     gene            8950..9360
                     /locus_tag="VHV99_00870"
     CDS             8950..9360
                     /locus_tag="VHV99_00870"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX3634046.1"
                     /translation="MRNHRAHYFGNEEKPRIEIIPMIDIMMFLLVFFMLVMLKMIQGT
                     GLKLELPQSSTATQLNTVDVTVGVEKDGELHLDRNDISAPQLTARLKDMEATKQVNVT
                     IAGDKGVEYETIIKTMDLVRSAGISSVALATDAQ"
     gene            9379..>9770
                     /locus_tag="VHV99_00875"
     CDS             9379..>9770
                     /locus_tag="VHV99_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634047.1"
                     /translation="MNMSISSKTQGALWPSMASQPDSGRAMTIAILAAVVIEGMLIAG
                     VMSVRVPAPKPVPAPRVMQMRVVKAVTPPPPPPPPPVIKKVVKQQSDLPVPKPVARPA
                     APARRHAAAPIAAPAPVAPTPVATQDAPS"
BASE COUNT         2056 a         2874 c         2872 g         1968 t
ORIGIN      
        1 ctcagcacga gttcccgttt gggccgtccg ctcatcgccg ctcctcgtat ctgtgccaca
       61 catagcactt tagtacgcgg cgaaaataat tcaatgaatt tgtaactcac tacactagga
      121 ttcggccacg gcgtcagcgt aaatatccac gcagccggct cagctacaga atggttcggc
      181 acagacttca acccgtctca tgctagcttt gcgcggcaac tagcgaagtg cgccggatcc
      241 caagcgaaat tgtccgctga aagcttcgcg aaattctgca cgcgagacga tttgccgggg
      301 tttgatttca tcggcatgca cggcatctgg agctggattt ccgacgaaaa ccgttcgctc
      361 attgccgatt tcatacgggg caaactcaat gatggcggcg tcgtgtatat gagctacaac
      421 acccagcccg gctgggccac aatgttgccg gtgcgcgaat tgatgcactg tcatttccag
      481 gcgagcgccc gcctaaatgc acacgacggc gtgcccactg aaacgcaagt ccagatgcac
      541 atcgcagcgg cgttagactt cgtaaagacc gtgattgcga cgcaacccgg ctacgcagtg
      601 gtcaacccac ttctgcctga acgtgtcgac gcattaggca acgagaaccc gcactacctc
      661 gcacatgaat attcaatcgc gactggcagc ccatgacttt ctcaaaggcc acatcgtcgc
      721 ttgccgcgag cggtttgact tatggctgct ccgcagacta ccgcgatcac gttgacgaaa
      781 tcaatctcaa cccagcacaa cgcgcgttgt tggcaggaat tccagatacc gctctacgtg
      841 agaccgtacg agacttttgc atcaaccggt cgatgcggcg agattactgg gtgaaaggaa
      901 gccaggcgct cagtgaagag gctcagcaga cggcgctgcg gacgcatcgc gtcatgctgg
      961 cgctgccaag ggatttggtc ttgctgaaag tgcgcggcgc gctgggagaa gcgacactac
     1021 cggaaagatt atatggtccg attctcgacg ggctagccag tcgtcatccc gcctgcctgg
     1081 ccgatatcga gaacagcgtc cgcggacgcg aaattacgct cgcccttatc gtcaaggccg
     1141 tgacattctt agtgggggcc ggcgtgttgc cgaatgtaca ggatgaggcg cacatagagc
     1201 tcgctcgtcc gtcatcgaac aggctgaatg ctgccatttg cgagaaggcg ctccagcatg
     1261 gggacgtaca atttttggtc agtccagtgt ccggatcggg cgttttgatt ccccgtgcgc
     1321 cacagctctt cctgttggcg aggcgacgcc acttgagtga accagcaaag tgggccgaat
     1381 tcgccgaggc tgccttgcgg gcgagtcccg cgccagctct cgtcgcggag ggcgcttccg
     1441 ttagcccgct caacaccagc ggcgatctga ttgtgaaagc gaatcgcttt accgaaatac
     1501 atctaccgat attgcaggcg ctgggaatcg cttgatgaaa ggatacgatc ggcgccctcg
     1561 tgaggttgcg caagcgccag agagccagct aactccactg cgtcgcgcga agatgtggcc
     1621 atgcagctga aatacccagg gcgttaggtt tgccctgaat tggcgcgagt cccccaccct
     1681 tcttgtgaca agcatataaa gggtgggaag gcgatggccg tgtagtctta cagcgccacg
     1741 aacagaacgc cgtgcggatc ttgcagcgca gaggcgcttc cgatgcctgc aactaccggc
     1801 gtcagatgac cagtcttgac gtccagctgc ataacctgcc cttgcccggc gctgtacgca
     1861 tcgccctgct tgaacggcgc atcgacgcgg taggtcgtgt tgctggcatc tgtgaagagc
     1921 atgaatgtcg ttcccttcgc tcccggcgcc ggaacccagc gggtatcgtc aaccggataa
     1981 gtcggcccgt ccttgtcgtt gtagagcgtc agcgacagga tgctgacgct ctggctcgca
     2041 cctgggttat ggatgaagac cagtttcccg tctgcctggc tgtccagtac cagatcgccc
     2101 gcggcgtcga tcgcttctga gtcaggatcg gtcaggttaa acgttatggg ctggttgaac
     2161 gtcggattcg cggagccgcc gactgatggc gtgatgtcga tcattgacgc gttgccggca
     2221 aggacggggg caacgtcaaa tgtcgtgcca ttcggattca acataagcga gacgacagtc
     2281 ggcgctgcgc ccggcgtgct cggggccgag gcactaacgt acacgactcc gttcatgagc
     2341 tgcatgtcgt cgaaaccgcc cccatgctct gtcgggttga ccgttgcctg ataggtcttc
     2401 tgagccccgg tcgcgagacc gatgatggtc agttcaggat ttccgtcttc gttcgacatc
     2461 gcccacagcg tgttcgtatc ggtgcgcgcc attaacccat cgacgtgccc gggtactgtg
     2521 tagcttttga gtacattgcc gcttaaatcg tactggacga ccgtattggt aagtcccgga
     2581 accgacccgt ccttgacgtc ccccgccatt tgatagccga tgaagatcgt gttgttgtat
     2641 tggacgatcg agtccggctt tgcgttcgcc gatggcgcct tggcaaagac cgacaacgaa
     2701 taacctgcag cagttgctgt gttaaggacc tgcgctgtat tggcgggcgc cacccccggc
     2761 gttgccgtga cgttgttgct gccgccacaa gcggaaagca gcactgcggc agaaattgcg
     2821 gacagaaaaa tcttcatctt catattggca tcctaaatga atggaaattt ttgtctcgat
     2881 gggcaggcga ctgtggccgc cgaacgaaat gacttcacct caaacagatg ccgccaaggt
     2941 gaacaccacg gtcacatgac aatcatatgc cgcaattgtg tccgcgtaat gaattgaaat
     3001 attttcgcgt gtcgatagaa gacgcgtcgc tccctgcctg atagccgaat gggaggccgg
     3061 tcatgctggt acgccgctat tagctatttt caatagtagt agtgccaatg aactattagt
     3121 tatcgggttc tgcccgtcga agcatgatcc gggttgcact ggaatgcggt gggaagcacc
     3181 gatcgatgca cgctccgcga acacacaggg aattcatgcg aacggcgacg agcacgttcg
     3241 ctctcgtcgc gggccgttag cgcaatcaaa aaccatcaga tcgtctcgca cccgtcctta
     3301 tggcaacctt aagagagcgc gaattggtaa aaatatgacc gcacctataa aaccgggccc
     3361 ggtaacttct ggaccatcga ctgcccgagc cagcaggacc aggtgcgtgg cagttgacac
     3421 ggcttgccaa cttgtcgagg ttgaggcgga aaggcggcag aaaagagcat ccgtcagaaa
     3481 gcccaggacg gatgtcggta acttcggtca tttcgcgcca cgcgacgaac tgtgctggga
     3541 gttgtttgcg aaagagtgaa gcgcgcagcg gatgccggtt gagcgcgaaa tgtagccgga
     3601 tcggcccgaa gcacgagaga gatttctgcg tgcgcttcgc gtcgcgaaag ccacgcatgc
     3661 gccgctcgca ttcgcgtgtc ggttggtggc cgttctctgc gcggttgttc agccgggcag
     3721 ccgccttgac gaacacgtgc ttcacattcg caagctccgc gatctcggct tttgccgccg
     3781 gatagctgcg caactgatcg gtgacgatct tgcgcggcac ggggttcgag cgcatgcctc
     3841 catgcatgtt atgaagcctg acgtcacccg tccgatgtcg tcacatgacg atatagaagt
     3901 taaattcaat ttatgcttca ggatcgagac aggcactctc gtatctaact aattgattta
     3961 cttaattatt attgcagcat cgtcggtgag atttcgtaat attttatctt ctgatgacga
     4021 tgaactgtcg ctaaattgtc aaaattgaca ctttggcgtc acagtagttc cgtaaaacgt
     4081 cttccgcatc gaaggcgatg cacgagggaa gtgggacgtg cgacgcatga catcgatccg
     4141 ggtaccgtcg atgggattct gcgcgataaa gagtgtcata tcattagtga tcctatctga
     4201 ttcactgagt cgcgcagctt cgtggaggcc gcctgaattt ctgcatacct gataccaggt
     4261 acatgtgtat ccgggtcagg atgtttgcaa cgattgacgg agaataaaca tgccgaacag
     4321 gcatatgaca gggttgttgg tgggactggt gagcggcgcg acaatgagcg gcgcgtatgc
     4381 ggcggatacg gcgacgttgc agggtcacgt gctgtcgagc gcgaacaagc gtccgctcgc
     4441 cggcgcccgg attcagattc gcgagacggg gacgagcacc accacctcgt ctgatggcgc
     4501 ttttagtttt accagtcttg ccgcagggca atacacactg gtcatcacgc cgactcgcgc
     4561 tgccgccgtc gaacgcactg tggtggtgcg cgagaacgac acgacaagcg acgacctcac
     4621 cgtggacgca gccgttccct cgctcgaccg cgtgaccgtc acgtcgcagc gcaccgcgaa
     4681 cgttgtcgcg cgggcgaccc agcagagcgc ggcgaacatg gtgaacatcc tgactgctga
     4741 cgacatccgc aagatgcccg acgtcagtgc tgcggaagcc gtcgctcgcg cgcctggcgt
     4801 gtcgcttgaa acggattcgg gtcaaggccg atacgtcaac attcgaggtc tcgataccaa
     4861 cctgaacagc acgaccttcg gcggcatcca tttgccgccg accaacccgg cttccgccca
     4921 gaacgggggg cgtgctgtcg cgctcgatac gattccggcc ggtatgatcg gacagatcgt
     4981 cgtgactgac accaacaagc ccgaacagga tgcggatgcg ctcggcggca cgatcgacat
     5041 ttcgcctcgc aagttgcccc cgtcgggcaa gccgttcctg gacgccaggc tcggttcagg
     5101 tgtcgagacg ctgcgcggta gcacaattca ggatgtcgag atgacagcgg gtgcgcgctt
     5161 cggcgcgcac ggcattgccg gtctcggttc gggcatcacg tcatattccg acgcgccgtt
     5221 caccttcatc ggtacctttg cctacgacca ggaccaccgc ggcgtcgacg acgaagaaaa
     5281 cgcctatatc gatgcgactg gcgtaccgaa caaggcctac gcgcaaaccg acccgcgtta
     5341 ttacaactat catcgcaagc gccttggcta cggcggcgaa ctggactttc agcctaacgc
     5401 cgacaacaag tggtatttcc gctactacaa cttcggctac acggagaatg tgaaccggca
     5461 ggatatccag accaacttca ctggcaatcc ggtccagaat gcggacggca cgctcacgga
     5521 tacggcgacc tatgcgaagg gattgcgcga cgagaaggag acgctgcgaa cgcaactctt
     5581 ttcgctgggc ggtgaaaaca acatcggccc cggcaagatc gattatcgcg ccggcttcac
     5641 cagtggcact tacgacaaac cgtacgacct gaattcgacg ttcaacaacg gcgccccggc
     5701 cacggtaacg tattttcccg gccccgactt cgcgacgcaa tcgatcgtgt ccggtccgaa
     5761 cccggctgat cccgcgggct acacgctggg ccagatcgtc aactcgtcgc agtacgtcaa
     5821 gaccaatgag tggtcggcca acgtcaacta cacgctgccg actcacttca cgagtgccga
     5881 agacgaacag ttcaagttcg gtgctggcct gcgccgaatg accttcgcca gcgcgcagag
     5941 caactacacg tactcgccgg cctcgggaat cccgctgtcg caggcggtgt tcggtgcgcc
     6001 gaacgttttc tacaacggca tgtacaacgt cgggccgtcc atcagcgacg gcgtcatgag
     6061 cggcatttat ggcgccgctg cggccaacgg caacctcacg gcttacccgt tgaaggacgc
     6121 cgccgcgttc tcggctatta acgagaacat ctacagtgcg tatggccagt atcagttcgg
     6181 ctggggccgc ttgggggtgc tggcgggtct gcgcggcgaa tggacgcagg atgcgttcag
     6241 cggcaatctg atcaacggta gctccgtcac gccaaaccag acgtcgaaca actacttcaa
     6301 cctgtttccg tccctgcagt tgcgctacga tttcacgccg acgttggtgg gtcgcgcgat
     6361 ctattcgagc acgatcgcgc gtccgggctt tgcccagagc agtgcgtcgg tcatggccga
     6421 tccgggaacg ggaaccgtga ccaccggtaa cccgaacctg aagccgacca cttcacagaa
     6481 cttcgatatt cagttcgaga agtatctgcc gcatggcggg atcatttcgc tcggcgcgtt
     6541 cgacaagtcg atgaccgact acgtcgtggg ccagtcatcg accgtcttca gctcgcctta
     6601 tctcccgggg tattcgggcc ccatcaggct cgtaagcttt gccaatgtct cgtcggcacg
     6661 tgtgcgcggt gtcaccgcaa actatgagca gcactttacg cagttgccgg ggttcctgag
     6721 cggcctgggc gcaggcgcga actggacctg gaccaattcg cacctggaca ttcgcccggg
     6781 cgacgaccac acgctgccat ccggtccgcg caattcttat aacgccagcg tgttctacga
     6841 gcgtggcccg ttggacatgc gcctgtcggc ctcctatgtg ggcaagagcc tgtgggcagt
     6901 gggcagttct gctgcaaccg acgtctacac ggcgtcgcgc ttctccctcg acttcggcgg
     6961 cagttatcag atcagaaagg atgtgggcat ctatctcgac gttcgcaaca tcctgaacac
     7021 gccgctgaag ttctacgagg gctcgccgga tcgtccgatc cagcgcgagt tctacggccc
     7081 gaccgtgatg gttggtttga cgatcaacga ataaccccgc aaaaacagga tcaacatgaa
     7141 actcaagtct attgttcagg cagtaacgac gctggctctg tcctccgcac tcctattggg
     7201 cagcacggcg catgcagtac cgggcgcgaa tcacggtgag ttcgaagtcg cgaagcacta
     7261 cgcgttgggc ggcacagagg gctgggacta cgtcgggtat gacgcggttc gtcaacacct
     7321 gttcatcagc cgggacagtc atgtccaggt tgtcgatacc ggatccggca agctggtcgg
     7381 cgaggtcgcc aacacggacg gcgtgcatgg cttcgcgttt gtccaggacc agaagctcgg
     7441 cttcatcacc aacggccgtg ccgacacgat tacggtggtc gatctcgaca cgctgcgcac
     7501 cgtcggaacg atgaaggcgg gcggcccgga cccggacggc atcatgtatg tgccccaatt
     7561 gaagcgcctc tatgtcagca atggccatga ccagagcgtc agcgcgatcg atccggttac
     7621 gcgcaaggtc gtcgcgacga tgccggtcgg aggcaagcct gaatcgctcg cggctgacag
     7681 cgacggtcgc atcttcgtca acgtcgaaga caagggagag atcgtcgcga tggatggaaa
     7741 atcgaacaag gtgctcgctc actggtcgat tgcgccttgc gaagggccgt cgggactcgc
     7801 gatcgatacg aagtcggaac ggctcttcac cgtctgcgcg aacaaccgga tgatggtggt
     7861 cgatgcgcgc tccggtcgcc aggttgccca actgccgatc ggcggccacc cggacgccgc
     7921 agcgttcgat ccggtgctca agaccgcgtt tagctcgaac ggcgcagacg gaacgctgac
     7981 cgtggtgcac gaagacgacg cggatcacta caccgtgctg cagaacgtgc cgacggaagt
     8041 gggcgcgaag tcgatggttc tcgacggcga tgctcatcgc gtctacctgg tctcgtcgaa
     8101 gttcggttcg actcccgccg cgactgccga caatccgcat ccgcatccga gcatcgtgcc
     8161 cggcacgttc aacgtcctgg tcgcgcagcg catgcagcaa agcagccagt aacgtatcgc
     8221 cgtcacctca accgcgccgc cggcggcatc gcaaccgcga tacgccggcc ggcggcagat
     8281 tcaagacaca acctggaagg tagcatcatg cttcgcatca ttatcgactc atcggtccag
     8341 tcattcggct tgttgccttt gatggcagtg atctttgtgg ccgcgctcgc gatcatcatc
     8401 gagcgcgcga ttttcttcgg cacgtccgtg cgcagcggcg tcagcctcga atacgacctc
     8461 aagcaggtag gccgcgccaa ggccgacgat gtgaagaaac tcgttcaaca ttaccaggga
     8521 tcggtgcaaa gcgaactgat gaggtccgcg atcgaatctc agggtcaacc tgtggaagac
     8581 ttcgagcgcg aggtcgaaga aagcatcatg ttccagatga gaaagctcga ccgcaacctg
     8641 tgggtgctgg ataccgcagt gaccctcggg ccgttgctcg gcctgctggg caccattatc
     8701 ggcatgattg gctcgttcaa tattctgggc tcgtcgggaa ccagcgatcc gatggccgtc
     8761 tccggcggca ttgcgcacgc gctgatcgcc accgcttgcg gcctgacgat cgctctcgtc
     8821 ggcgtcgtgg cgctcaacta tctgaacaag cgcgtgcgaa tggttcttct gcagatggac
     8881 atcatcaagt cgatgctcgt cacgcggctt gccgcttgag tcgcagccac ttcacatacg
     8941 gagaacaccg tgagaaacca tcgggctcac tactttggca atgaggagaa accacggatc
     9001 gagatcattc cgatgatcga catcatgatg tttctgctcg ttttcttcat gcttgtcatg
     9061 ctcaaaatga ttcagggcac cggacttaag ctggaattgc cgcaatcgtc caccgcgacc
     9121 caactcaaca cggttgacgt gacggtgggg gtggagaagg acggagagct gcatctcgac
     9181 cggaacgaca tttctgcgcc gcaacttact gcacgcctga aggacatgga ggccacgaag
     9241 caggtcaacg tgacgattgc gggcgacaag ggagtcgagt acgagacgat catcaaaacc
     9301 atggatctgg tgcgctctgc ggggatcagt tcggtcgcac tcgcgaccga tgcgcaatag
     9361 tctctggagt cgagcgtaat gaacatgagt atctcgagca aaacccaagg agcactgtgg
     9421 ccgagcatgg cgtcacagcc tgattcgggc cgtgcaatga cgatcgcgat tctcgctgcg
     9481 gttgtgatcg agggaatgct gatagcgggg gtgatgtcgg tgcgtgtgcc cgctccgaag
     9541 ccggtgcccg cgccgcgagt catgcagatg cgggttgtca aagcggttac accgccgcct
     9601 ccgccccctc caccgccggt catcaagaaa gtggtgaaac agcaatcgga ccttccggtg
     9661 ccgaagccgg tggcacgacc ggccgcaccg gcacgacggc acgctgccgc gccgatagca
     9721 gcaccggcgc cggttgctcc cacgcccgtc gcgacacagg atgcgccttc
//
LOCUS       DATASH010000029        13287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6729379,
            whole genome shotgun sequence.
ACCESSION   DATASH010000029 DATASH010000000
VERSION     DATASH010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 13287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13287
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6729379"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="VHV99_00880"
     CDS             complement(<1..454)
                     /locus_tag="VHV99_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HEX3634048.1"
                     /translation="MRLTTKGLLLIAIPALFELALLSGLVKAQADATQAERWAIHSQD
                     VLRQTTAILEPVLGESVALRGAVLANDARFATPVTVWMDVDRRIDQLAELVADNPAQV
                     ERVVQVRQAVQAYRQWSDRIQDMLHSGRRRDVLARFRDLAPSDVLDRFR"
     gene            716..859
                     /locus_tag="VHV99_00885"
                     /pseudo
     CDS             716..859
                     /locus_tag="VHV99_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
     gene            complement(881..1228)
                     /locus_tag="VHV99_00890"
     CDS             complement(881..1228)
                     /locus_tag="VHV99_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007745748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HEX3634049.1"
                     /translation="MMKGYQLTFYTEQNRRHGHQTVVECLLSIAKHVGIHGATVVAAA
                     EGIGHAGARHAARFFELADQPQQVIFAVTEGEAEALLEAVRESNVAVFYTRCPIEFGM
                     LGAEPATKHPARR"
     regulatory      1404..1464
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            1542..1922
                     /gene="crcB"
                     /locus_tag="VHV99_00895"
     CDS             1542..1922
                     /gene="crcB"
                     /locus_tag="VHV99_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEX3634050.1"
                     /translation="MYWSILSVGVGGALGSLFRWFLGIRLNGVFTGLPLGTFAANVIA
                     GYVIGVAVAGFARAPQIAPEWRLFVITGLMGGLSTFSTFSAEVVQRLQDGRLGWAAGE
                     VVIHVGASLLMTILGIATVSLLAR"
     gene            complement(2027..2599)
                     /locus_tag="VHV99_00900"
     CDS             complement(2027..2599)
                     /locus_tag="VHV99_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX3634051.1"
                     /translation="MRDRRSTRAAALTALLSLSALASSCSSIAPGELNAGTRYGIGTP
                     LTDQDLAAWNIDIAPDGRGLPAGSGDVATGAHLFAAKCAACHGAQGEGGLGDQLVGGQ
                     GTLISAKPKRTVGSYWPYATTLFDYIRRAMPYNAPESLSADEVYAVSAYLLNQNGIVP
                     ANTRLDAATLPRVVMPNRQGFVADPRPGRL"
     gene            complement(2592..3857)
                     /gene="soxC"
                     /locus_tag="VHV99_00905"
     CDS             complement(2592..3857)
                     /gene="soxC"
                     /locus_tag="VHV99_00905"
                     /EC_number="1.8.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite dehydrogenase"
                     /protein_id="HEX3634052.1"
                     /translation="MNPHESPPSRGRRRLLGRLAAGGLAVSHVAAASELLKPLEVAPS
                     SRVPGAPILEHPYGVPSVHEADVVRRSARAWPLPGAASSMTPLADLHGTLTPNGLVYE
                     RHHGGVPDIDPDQHRLAIHGLVRTPKLFTIDDLLRLPSESRIHFLECSGNTGNEWNGP
                     SGMPVQLTHGLLSCCEWTGVRLSTLLEEVGGVTAGGGASGSGWILAEGADAAAMTRSL
                     PLDRILDHALVVYAQNGERLRPENGYPLRLIVPGFEGNTNVKWLRRLKVVDAPLQTRE
                     ETSKYTSLLANGTARQFAFEMDAKSVITRPSPGHRLTTHGYYPITGLAWSGRGTIRKV
                     EVSADGGATWHLARLDGAVRDRALTRFQADWRWDGGAAAILSRATDSTGYVQPTRAEL
                     VAARGLNSQYHYNAIQQWRVEASGEVRNA"
     gene            complement(3983..4129)
                     /locus_tag="VHV99_00910"
                     /pseudo
     CDS             complement(3983..4129)
                     /locus_tag="VHV99_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            4383..5450
                     /locus_tag="VHV99_00915"
     CDS             4383..5450
                     /locus_tag="VHV99_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012217251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3634053.1"
                     /translation="MRYFRLLKTFIAVATLSLAAVHAYADKPAVIRIGVAQQGTGDPP
                     TFGGSPAATVQLQQSLEKEFAADGIKIEWLFFKGAGPAVNEAIADKSLDFAFQGDLPA
                     VLARANGLKTHLLLASGVRTGIKVAVPTDSDVHSIKDLKGRRVSIFRGTNLQLVADNA
                     LAANQLDERDLRVINLDTASSLAALASRGIDASVNDYHVYKLRDQGLAKVVYESQNDG
                     PQFTRQSHLLVVDDFDRAHPDIVQRVVNVFVKGSQWSSDEANRDALFKLWAKSGVPYS
                     SWQAEYANQKLKDRNSPLIDPFIVARYKAVAADALKLKLIRQPVEVDGWFETKYLDNA
                     LSTLKLDHYWTRYDAAGKPLS"
     gene            5467..6330
                     /locus_tag="VHV99_00920"
     CDS             5467..6330
                     /locus_tag="VHV99_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3634054.1"
                     /translation="MSETALPVDNKRLGTAAVAPAASHPPLHRNPRLRALAWRLAPWV
                     LPAVLFALWTVGCERGWIAPQILPPPERVFDTFVELAKSGDLASNTLISLQRVLLGFA
                     AGTVVGFVLGAALGFSRTFEAYVLPSFNALVQIPVLGWLPFLLLLVGVGEPLKYILIA
                     HAALVPVTLSTLQGFRQTPASLNEVARVFEYNGWQRIVYLVLPAAVPTLATGVRLAFT
                     KAWLALVVVELVASSEGLGYLIVYGRQLFQLDLVMASVVVVGAIGYAVNRSLDALEAR
                     LRRGQPSAFRE"
     gene            6383..7252
                     /locus_tag="VHV99_00925"
     CDS             6383..7252
                     /locus_tag="VHV99_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3634055.1"
                     /translation="MSLFSFTRSKAAADTPCDCNVPAAFRRAARPRRSLLARVDWRGA
                     VLPIVAIAIWWAVSEAHLVKSGLLVSPAQVLATAWQQIISGALFKALCASLAREASGF
                     VIGTLGGLLLGSVLGFSRLATRMIGPSFDTFKQISLFAWIPLISVWFGLGDAAKVVFL
                     SLAALLPVTAHTCDGIHAVPRAYIEVARAFRYTRWQLIRAVILPAALPSIFSGIYLAL
                     IYSWLATLGAEYLLVAGSGIGNTLIDGSEQFRMDLVLFGVIVVGVTGWALNALARAVE
                     RAIFARRSPQAAR"
     gene            7287..8162
                     /locus_tag="VHV99_00930"
     CDS             7287..8162
                     /locus_tag="VHV99_00930"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3634056.1"
                     /translation="MTSSTLSESIDILRVSKRYAQQDGTPLAVLDDITLRVRPGEFVS
                     VLGSSGCGKSTLLRLIAGLDTDYTGDIVVAGEKVRDTSLERGIVFQDHRLFPWLTASQ
                     NILAALRNAPLSAREKRDAVAQHIALVGLNGFENAYPHQLSGGMAQRVAIARGLVNRP
                     RVLLLDEPFGALDALTRARLQNELQRIWEHERITMILVTHDVDEALYLGDRVVTMAPR
                     PGRIKRIVDVALPRPRERSDPRFIRLRDEVLADFAEHDGQRREPAEDDTPGGGTRLQT
                     APAASPPITEWRLAW"
     gene            8372..9319
                     /locus_tag="VHV99_00935"
     CDS             8372..9319
                     /locus_tag="VHV99_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX3634057.1"
                     /translation="MSAAAVQDIQSIQANPLSAHIGAEIHGVDLTQKLDTRQIAEIRA
                     ALLKWRVVFFREQFLTHEQHVAFSAQFGELTLGHPVFGHVDGHPEIYSISKYRKATRF
                     EGQALLRPWTGWHTDVTAAVNPPWASILRGVTIPPYGGDTQWTNLVAAYQKLSAPLRT
                     FVDGLRGLHRFTPPAGASGTEAFAKAVEQRILVTEHPLVSVHPETGERALYVSPSFLK
                     SIVGVTPRESQVLLELLWEHVTRPEFTVRFKWEAGSVAFWDNRATAHLAPTDIFDLDF
                     DRQLFRTTLVGDVPVGPDGAQSVAIEGSPVSAAAAVALN"
     gene            complement(9479..10822)
                     /locus_tag="VHV99_00940"
     CDS             complement(9479..10822)
                     /locus_tag="VHV99_00940"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX3634058.1"
                     /translation="MSKRSGHLRLGAFLYPSGHHIAAWRHPGAQADAGVNFRHYVQLA
                     QAAEAAKFDLIFLADGVGTRGDNVDFLSRTAHSYVAQFEPITLLSALAAVTERIGLVG
                     TASTSFNEPYHIARKFASLDHISGGRAGWNLVTSSSEHEARNFNRDKHFDHAERYVRA
                     AEFAEVTAGLWDSWEDGAFVRDKAEGRFFDPAKRHVLDHKGRFFQVKGPLNVARSPQG
                     HPVVVQAGSSEADRELAARTAEVIFTAQQTLRDAVDFYADVKGRMQAYGRHPDDLKIM
                     PGVFPIVGRTESEAKEKFEQLQSLIDPKVGLALVSGLTGGFDLSEYPLDGPIPELPET
                     NASKSRQVLTIDLARRENLTIRQLYLRVAGARGHWQLVGTAAQIVDQLEERFVNHGAD
                     GFNVMPPVLPDGLTDFVELVVPELRRRGLFRENYEGRTLRENLGLRRPENRCFPA"
     gene            complement(11483..12727)
                     /locus_tag="VHV99_00945"
     CDS             complement(11483..12727)
                     /locus_tag="VHV99_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HEX3634059.1"
                     /translation="MALKIEDLPSAIRQAKSALRAALPNYREVFAEVEEAISAEAKRI
                     AGQRDRGEDVIPEIQFSDITEQRVTADQIALVKARGACVIRNVFPRALVQSWDEEIAG
                     YVERNHLDKRLENRAEDKYFGQLASSKPQIYGVYWSKPQVLARQSESLTAARVFLNRL
                     WRNESEGRVHFDPARVPVYADRLRRRPPGSESLGLSAHCDGGSVERWIESNFRKVYRH
                     VFAGNWRDYDPFDAAFRPDVEEIASPAVCSMFRTFQGWTALTPQGPGDGTLQLVPIAN
                     SMVYILLRALQDDVAEDDLCGAMPGRALSIKREFHAPLFEALASIPKMEAGDTVFWHS
                     DVIHAVEDEHKGAGYSNVMYIASAPACAKNDAYLRRQLPSFLEGESPPDFPADHFEVD
                     FTGRATADDLTPLGKAQLGFGL"
     gene            12821..>13287
                     /locus_tag="VHV99_00950"
     CDS             12821..>13287
                     /locus_tag="VHV99_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007583174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX3634060.1"
                     /translation="MKPAYEHVEFGEDCSLRVYHRRLPRIPFEWHHHPEYELTLTMNS
                     HGKRYIGDSVNDYAAEDLVLVPPDLPHTWASNRSIEPSSPQVAIVIWFDGDWARRMAD
                     CCPEYEPLRKLLRRAACGLAFEPPAGQWMRGQLDSLLSSGARERLGAALDILCT"
BASE COUNT         2389 a         4341 c         4033 g         2524 t
ORIGIN      
        1 gacgaaaccg gtcgagcaca tcggacggcg cgagatcacg gaagcgcgcg agcacgtcgc
       61 gtcgccggcc cgaatgcagc atgtcctgaa tgcgatccga ccattgccgg taagcctgca
      121 ccgcctggcg cacctggacc acgcgttcga cttgcgccgg attgtcggcg accagttcgg
      181 ccagttgatc gatgcgccgg tcgacgtcca tccacaccgt caccggtgtg gcaaaacggg
      241 cgtcgttggc aagcaccgcg ccgcgcagcg ctaccgactc gcccagcacc ggctcgagga
      301 tcgctgtggt ctgacgcagc acgtcctggc tatggatggc ccagcgctcc gcctgcgtcg
      361 catccgcttg cgccttgacc agtccggaca gtagcgccaa ctcgaagagc gccggaatcg
      421 cgatcagcaa caggccttta gtggtcagtc tcatgcgtac gcattggaag tcgatggccg
      481 gcagatcatg cgcgggaacg gcacattatg tcggcgtttg cacaggaaga caaagacgac
      541 gcgccacgaa tcatggcttt tatcgcgtga tcaaccccct cgtgccacga atgcgcacaa
      601 aacgtcggca ttttgaatta aacgtaaagt aaatcgaata ttttgcccag gctttaacgt
      661 gatgagcgcg cggaccatcg acaaattcag cgcccttgta gccgtcaatt cgctgatggc
      721 gatactcggc ggcacgcttg tgtacggagc gattcgcctg acggtgggct tgcggctcga
      781 ccaggaagac gaatgcaacg gcgcggatct gtcgatccac aagatttggg cgacgcccgc
      841 gcgcgaaagc gtcggctgat gggtttgccg cgaagcagcg ttagcggcgc gccggatgtt
      901 tcgtggccgg ctctgcgccg agcatgccga attcgatcgg gcaacgcgtg tagaagaccg
      961 cgacgttgct ctcacgcacc gcctcgagca acgcttcggc ctcgccttca gtgaccgcga
     1021 aaatgacctg ttgcggttga tcggccagtt cgaagaagcg cgcggcatgc cgtgcgcccg
     1081 cgtggccgat gccttcggcc gccgccacga cggtcgcgcc atgaatgccg acgtgcttcg
     1141 cgatcgacag cagacattcc acgaccgttt gatgtccatg gcgcctgttc tgctcggtat
     1201 agaacgtgag ctggtagccc ttcatcatgt cggcctcccg cgcggccgca gtcaaaccca
     1261 cggcgaatgc tcgcgggttc tctcttcatt gtaggtcacg ctccgggcgc gtctcgcgga
     1321 ccacacaggc tcgctcccgt cacgtgcgcg ctcacattgc gccgccttcc acctgccccc
     1381 caatcgggcc gccgatgcta gacttgcgcg ccggagatgg cattctcctt aaccgccctt
     1441 gtggctgatg atgcctgctt cgcccggatg actgcggacg cggcatcgca cgtcccgttc
     1501 agcccggttt gacgaccttt tcgcttcgcc agggcattct catgtattgg tccattctct
     1561 ccgtcggcgt cggcggcgcg ctcggctcgc tgtttcgctg gttcctcggc atccgtctga
     1621 acggcgtgtt caccggcttg ccgcttggca ctttcgccgc caatgtcatc gccggctacg
     1681 tgatcggcgt cgcggtcgcg gggttcgcgc gggcgccgca gatcgcgccc gaatggcggc
     1741 tcttcgtcat caccggtttg atgggcgggc tgtcgacttt ctcgacgttc tcagcggaag
     1801 tcgtgcagcg cctgcaggac ggccggctcg gctgggcagc cggtgaggtg gtgattcatg
     1861 tgggcgcgtc gctgctgatg accatcctcg gcatcgcgac cgtttccctg ctggcgcgct
     1921 gaaagccggg agcccagccc agatgcttcc atgaccttga ccgccgggcc ggacgcgata
     1981 cctgccggcg tgtgtcgcca tctgtccctg ggcgcgcacc cacggctcac aatcgtcccg
     2041 gacgaggatc ggccacgaag ccctggcgat tgggcatcac gacacgcggc agcgtggccg
     2101 cgtcgagccg cgtattcgcc ggcacgatac cgttctggtt gagcaggtag gcgctcaccg
     2161 cgtacacctc gtcggcggaa agcgattcgg gggcgttgta gggcatggcg cggcggatat
     2221 agtcgaacaa ggtggtcgca tagggccagt agctgcccac ggtgcgcttc ggtttggcgc
     2281 tgatcaatgt gccctgcccg ccgaccagtt gatcgccgag accaccctca ccctgcgcgc
     2341 cgtgacacgc cgcgcatttg gcggcgaaca ggtgcgcgcc ggtagcgaca tcgccgctgc
     2401 ccgccggcag gccgcggcca tccggcgcga tgtcaatgtt ccaggcagcg agatcctggt
     2461 ccgtgagcgg cgtgccgatg ccgtaccgag ttccagcgtt tagctcgccg ggcgcgatcg
     2521 atgaacagga ggatgccagc gccgagagcg acaggagtgc cgtcagtgcc gccgcgcgcg
     2581 tcgaacggcg atcacgcatt gcgcacctcc ccgctcgcct cgacacgcca ttgctggatc
     2641 gcgttgtagt gatactgcga attcagtccc cgcgcggcga cgagttcggc gcgcgtgggt
     2701 tgcacatagc cggttgagtc cgtggcgcgc gagagaatcg cggcagcgcc gccgtcccat
     2761 cgccagtccg cctgaaagcg cgtcagcgca cgatcgcgga ccgcaccgtc cagacgcgcg
     2821 agatgccacg tcgcgcctcc atcggccgac acttcgacct tgcggatggt cccgcgcccc
     2881 gaccaggcga gacccgtgat cgggtagtaa ccgtgcgtgg tgagccgatg tcccggcgac
     2941 ggccgcgtga tcaccgattt cgcgtccatc tcgaacgcga actgacgcgc ggtgccgttc
     3001 gcaagcaagc tcgtgtattt cgaggtttcc tcgcgcgttt gcaacggcgc gtcgaccacc
     3061 ttcagccgac gcagccactt gacgttcgta ttgccttcga agccgggcac gatcagacgc
     3121 aacggatagc cattttccgg gcgcaagcgt tcgccgtttt gcgcatagac cacgagcgcg
     3181 tgatcgagta tccgatccag cggcaagctg cgcgtcatcg ccgcggcgtc cgcgccttcg
     3241 gccaggatcc agccactacc gcttgcgccg ccgcccgccg tcacaccgcc cacctcctcc
     3301 agcaaggtgg ataaacgcac ccccgtccat tcgcaacacg acagcagacc atgcgtaagc
     3361 tgcaccggca tgccgctcgg gccgttccat tcgttgccgg tattgccgga acattcgaga
     3421 aaatggatgc gcgactctga cggcaggcgc agcagatcgt cgatcgtgaa cagcttcggc
     3481 gtgcgtacca gaccgtgtat cgccagccga tgctgatccg gatcgatgtc gggcacgccg
     3541 ccgtgatgac gttcgtacac caaaccgtta ggggtgagcg tgccgtgcaa gtcggccaat
     3601 ggcgtcatcg aagacgccgc gccaggcagc ggccacgcac gcgcgcttcg gcgcacgacg
     3661 tccgcctcgt gcactgaagg cacaccgtac ggatgctcga ggatcggcgc gcctggtacg
     3721 cggctcgatg gcgccacctc gagcggtttg agcagttcgc ttgccgccgc cacgtgtgaa
     3781 acagcgagac cgccggccgc gaggcggccc agcaatcgac gacggccgcg cgacggcgga
     3841 gattcatgcg gattcatata gtcaatggca acgcgtcata ggcaagcccg ctagcgtaat
     3901 gaagccgatc gcggtggcca aacgataaat tcatatatcg atcgacataa accgcatggc
     3961 gcacagacgg cgcgcgccgc caagccagca cgttcgagaa caccccatcc gccaggcggt
     4021 tcgaatcggt cccgctgttg agtacgccga ccgcgttcag atcatcggca caaagcgtga
     4081 tttccttcct gaacgcgtcg ccgaccggat gttggcggtc tgattcgctg cccgaacagg
     4141 cgattcgccg atatagcgtc atcgcacaaa actaacccac gagaatgatc gcaatagccg
     4201 ctgccgctca aaacgccggt gtccgacgcc atagccacgc agttagccgg ttcgaactac
     4261 tgcatatttt cttatcagca atgattggtt ggattcgagc gcacgcgaat ctaacgtgta
     4321 gtgccagtta tcggcccacc tgaattcgca acccgctccc ttccttcgat tggtattgcc
     4381 gaatgcgcta tttccgtctg ctgaaaactt tcattgccgt ggcgacgctg tcgcttgccg
     4441 cggttcacgc ctatgctgac aaacccgcgg tcatccggat cggcgtcgcg cagcagggca
     4501 ccggcgatcc gcctaccttt ggcggctcgc ccgcggccac cgtgcaattg cagcagtcgc
     4561 tcgaaaaaga attcgccgcc gatggcatca agatcgaatg gctgttcttc aaaggcgccg
     4621 gccccgcggt caacgaagcg atcgccgaca agtcgctcga cttcgccttc cagggcgatc
     4681 tgcccgcggt gctcgcgcgc gccaacggcc tgaagaccca tcttctgctc gcctcgggcg
     4741 tgcgcaccgg catcaaggtc gccgtgccta ccgactccga cgtgcactcg atcaaggacc
     4801 tcaagggccg ccgcgtatcg atctttcgcg gcaccaatct gcaactggtc gccgacaacg
     4861 cgctcgccgc caatcagctc gacgaacggg atctgcgcgt gatcaatctc gataccgcca
     4921 gttcgctcgc cgcgctggca tccaggggta tcgatgcgtc ggtcaacgat taccacgtgt
     4981 ataagctgcg cgaccagggg ctcgccaagg tcgtctacga atcgcagaac gacgggccgc
     5041 aattcacgcg tcaatcgcat ctgctggtcg tcgacgactt cgaccgcgcg cacccggaca
     5101 tcgtgcagcg cgtcgtcaac gtgttcgtca aaggctcgca atggtcctcg gatgaagcga
     5161 accgcgacgc gctcttcaag ctctgggcca aaagcggcgt gccctattcg tcgtggcagg
     5221 ccgaatacgc gaatcaaaag ctgaaagacc gcaattcgcc gctgatcgac ccgttcatcg
     5281 tcgcgcgcta taaagccgtc gcggcggatg cattgaaact caaactgatc cgtcagccgg
     5341 tcgaggtcga cggttggttc gaaacgaaat atctcgacaa cgcgctaagc acactgaagc
     5401 ttgaccacta ctggacgcgt tacgacgccg ccggcaagcc gctcagctaa aacgggaacc
     5461 atcacgatga gcgaaaccgc actcccggtc gacaacaaac ggctcggcac cgccgccgtg
     5521 gcgccggctg cctcgcatcc gcccctgcat cgcaatccac ggctgcgcgc actcgcctgg
     5581 cggcttgcgc cgtgggtatt gcccgctgtc ctgttcgcct tatggacggt gggttgcgag
     5641 cgcggctgga ttgcgccgca gatcctgccg ccgcccgagc gcgtattcga caccttcgtc
     5701 gagctggcga agagcggcga tctcgcgagc aacacgctga tcagtttgca acgcgtgctg
     5761 ctcggcttcg ccgcgggcac cgtggtcgga tttgtgctcg gtgcggcact cggtttttcg
     5821 cgcaccttcg aagcgtatgt cctgccgagt ttcaatgcgc tcgtgcagat tccggtactc
     5881 ggctggctgc cgtttctgct gctgctcgtc ggcgtcggag agccgctcaa atacatcctg
     5941 atcgcgcatg cggcgctggt tccggtcacg ttgagcacgc tgcaaggatt ccgccagacg
     6001 cctgcctcgc tcaacgaagt cgcgcgggta ttcgaataca acggctggca acggatcgtc
     6061 tatctggtcc tgcccgccgc ggtgccgacg ctcgccaccg gcgtgcgcct cgccttcacc
     6121 aaagcctggc tcgcgctggt ggtggttgaa ctggtggcgt cctccgaagg gctcggctat
     6181 ctgatcgtct atggccggca actgtttcag ctcgacctgg tgatggcctc ggtggtcgtg
     6241 gtgggtgcca ttggttacgc ggtgaaccga tcgctggacg cactcgaagc gcgtttgcgg
     6301 cgcggacaac cgtcggcatt tcgcgagtga tcgcgggtga gccgacccac ggcacccagg
     6361 cgccgcattt gaggaatcta cgatgagtct cttctccttc acccgctcga aggccgccgc
     6421 cgacacgccc tgcgactgca acgtgcccgc cgcttttcgt cgcgcggcgc ggccacgccg
     6481 gtcgctgctt gcgcgcgtcg actggcgcgg cgccgtcttg ccgatcgtcg ccattgcgat
     6541 ctggtgggcc gtctcggagg cgcacctggt caaaagcggc ttgctggtca gtcccgcgca
     6601 agtgctcgcc accgcatggc aacagatcat tagcggcgcg ctgttcaagg cgctctgcgc
     6661 ctcgctcgcg cgcgaagcga gcggcttcgt gatcggcacg ctgggcggcc tgctgctcgg
     6721 cagcgtgctg ggcttctcgc ggctcgccac acgcatgatc ggcccgagtt tcgacacgtt
     6781 caaacagatc tcgctatttg catggatccc cttgatctcc gtgtggttcg gtctcggcga
     6841 tgcggccaaa gtcgtcttcc tctcgctcgc cgcgttgctg cccgtcaccg ctcacacgtg
     6901 cgacggcatc cacgcggtgc cgcgcgctta tatcgaagtc gcgcgcgcct tccgttacac
     6961 gcgctggcaa ctcattcgcg ccgtcattct gccggccgcg ttgccgtcga tcttctcggg
     7021 catctacctc gcactgatct actcgtggct cgccacgctc ggcgccgaat atctgctggt
     7081 agcaggcagc ggcatcggca atacgctgat cgacggcagc gagcagttcc gcatggacct
     7141 cgtgctgttc ggcgtgatcg tggttggcgt gaccggctgg gcattgaacg cgctggcgcg
     7201 cgccgtggag cgggcgattt ttgcacgtcg ttcgccgcaa gccgcacgat aaacacgtac
     7261 ctgacctctc cttaccggaa ccgctcatga cttcttcgac gctttccgaa agcatcgaca
     7321 ttctccgcgt cagcaagcgc tacgcgcagc aagacggcac cccgctcgcg gtgctcgacg
     7381 acattaccct gcgcgtgcgt cccggcgaat tcgtcagcgt gctcggttcg agcggctgcg
     7441 gaaagtcgac gctgttacgg ctgatcgcgg gactcgacac cgactacacg ggcgacatcg
     7501 tcgtcgcggg tgaaaaagtg cgcgacacct cgctcgaacg cggcatcgtg tttcaggacc
     7561 atcgtctgtt tccgtggctc acggcgtcgc agaacattct cgccgcattg cgtaacgcgc
     7621 cactatccgc acgcgaaaag cgcgatgcgg ttgcacaaca tatcgcgctc gtcggcctga
     7681 acggcttcga aaatgcgtac ccgcatcagc tgtcgggcgg catggcgcag cgcgtggcga
     7741 tcgcgcgcgg ccttgtgaac cgcccgcgtg tgctgctgct cgacgaaccg tttggcgcgc
     7801 tcgatgcgct cacgcgcgcc cgcttgcaaa acgagttgca acgcatctgg gaacacgagc
     7861 gcatcacgat gattctcgtc acacacgacg tcgacgaagc gctttacctc ggcgatcggg
     7921 tcgtcacgat ggcgccacgg ccgggccgca tcaaacggat cgtcgatgtc gcgttgccgc
     7981 gtccacgcga acgaagcgac ccgcgcttca tccgtctgcg cgacgaggtg ctggccgatt
     8041 tcgccgagca tgacggccag cgccgcgagc cggccgaaga cgatacgccg ggcggcggca
     8101 cgcgccttca aaccgcgccc gcagcgagcc cgccgatcac cgaatggcgc ctcgcgtggt
     8161 gaagcgcgct gcttccctca agcacaccaa acaatcacgg agaaggccgg aactgcaacg
     8221 gcgtggactc tcgcgcaccg aatacgaagg ccgcaagctg cgcgagaacc tcggcttgcc
     8281 ccgacccgaa aatcgctatg ccgccgagac aaaacagata cccgcggtag cgggttattg
     8341 acgcgagacg tttttatcat aaggagcccc catgagcgca gccgcagtgc aggacataca
     8401 gtcgattcag gctaacccgc tttccgcgca tattggcgct gaaattcacg gtgtcgatct
     8461 cactcagaaa ctggacacgc gccagatcgc ggagattcgc gcggcgcttc tgaagtggcg
     8521 cgtcgtgttc tttcgagagc agttcctgac gcacgagcaa cacgtcgctt tctcggcgca
     8581 gttcggcgaa ctgaccttag ggcatccggt attcggccat gtcgacggtc atccggaaat
     8641 ctattcgatc tcgaagtatc gcaaggcgac gcgtttcgaa ggtcaggcac tgctgcggcc
     8701 gtggaccggt tggcacacgg acgtgaccgc cgcggtcaat ccgccctggg cgtcgatcct
     8761 gcgtggcgtg acgattccgc cttatggcgg cgatacgcag tggaccaatc tggtggccgc
     8821 ctatcagaaa ctttccgcgc cgctgcgcac attcgtcgac ggcctgcgag gactgcatcg
     8881 cttcacgcca ccggctggtg cgagtggcac agaggcattt gccaaggccg tcgaacaacg
     8941 tattctcgtc accgaacatc cgctcgtcag cgttcatccg gaaacgggtg agcgggcgct
     9001 ctatgtgagc ccgagtttcc tgaagtcgat tgtcggcgtc acgccacgcg aaagccaggt
     9061 gctgcttgaa ttgctctggg agcacgtcac ccgaccggag ttcaccgtgc gcttcaagtg
     9121 ggaagcaggc agcgtcgcct tctgggacaa ccgcgcgaca gctcacctcg cgcccacgga
     9181 tattttcgat ctcgacttcg accggcagtt gtttcgcaca acactggtcg gtgacgtgcc
     9241 cgtcggcccc gacggtgcgc aatcggtggc gatcgaaggc tcgccggtga gcgccgcggc
     9301 ggccgtcgcg ctgaactgag tacaacgccg gcgtacaacc gccggtgtct tccgcatcgc
     9361 acgtgcccga gcgtcgacgc tttagccgca cacgcacgcc gtgagctttt cagcatgcgc
     9421 accggcgtgc cgcaacagaa gtaagcgcga tagtgtgcgc tccgtttccc gctttctgtt
     9481 acgcaggaaa acaccggttc tcaggccgcc gcaaacccaa attctcgcgc aaagtacggc
     9541 cttcatagtt ctcgcggaac aacccgcgtc gccgcagctc gggcaccacc agttcgacga
     9601 aatcggtcag gccatcaggc agtacaggtg gcatcacatt gaacccgtca gctccatgat
     9661 tcacgaagcg ctcttcgagc tgatcgacga tctgggctgc ggtgccgacg agctgccagt
     9721 ggccacgcgc acccgcgacg cgcaaataga gctggcggat cgtgaggttc tcgcggcgag
     9781 ccagatcgat tgtcaacacc tgccggcttt tgctcgcatt cgtttcgggc agttcgggaa
     9841 tcgggccgtc gagtgggtat tccgagaggt cgaacccgcc ggtcagcccg gataccagtg
     9901 ccagtccaac cttcggatcg ataagcgact gcaactgctc gaacttctct ttcgcctcgc
     9961 tttcggtgcg accgacaatc ggaaacacgc ccggcatgat cttcagatcg tccggatgac
    10021 ggccataggc ctgcatgcgc cccttcacgt ccgcgtaaaa atccaccgcg tcccgcagcg
    10081 tttgctgcgc cgtgaaaatc acctcggctg ttctggcggc caactcccgg tcagcttcgg
    10141 acgacccggc ctgcacgacg accggatgcc cctgcggaga acgggccacg ttcagcggcc
    10201 ccttcacctg gaagaaacgt cccttgtgat ccagtacgtg ccgtttggcg ggatcgaaaa
    10261 agcgcccttc ggccttgtcg cgaacgaaag cgccgtcttc ccagctgtcc caaagtcccg
    10321 ctgtgacttc tgcgaattcg gccgcgcgca cgtagcgctc ggcgtgatcg aaatgtttgt
    10381 cacggttgaa gttcctcgct tcatgctcgc ttgaggaggt gaccagattc caccccgccc
    10441 gccccccgct gatgtggtcg agcgaggcga acttgcgggc aatgtgatat ggctcgttga
    10501 aactcgtcga ggccgtgccg accagaccga tacgctcggt cacggccgcc agcgccgaca
    10561 gcaaggtgat cggctcgaac tgcgccacgt agctgtgcgc cgtgcggctg agaaagtcga
    10621 cgttgtcgcc gcgggtgccc acaccgtcgg cgagaaagat cagatcgaat ttcgcggctt
    10681 cggctgcttg cgcgagctga acatagtgac ggaagttaac gccggcgtcc gcctgtgccc
    10741 cgggatgccg ccatgccgca atgtggtgcc cggaagggta aaggaaagcg ccgagacgca
    10801 gatgcccgct acgtttgctc atggaagttc gcgaaagaga atggccaggg agatcccgca
    10861 ctgcgtcgat cggccagatg aaccgctaga cgcgccggat gctcaacggg ttgacgttag
    10921 cgcgccgcgt ggtgcgcggc aaccaagcaa agctcataac cttatgaact ggggaaggtg
    10981 cgcgtcgcgt cgaatcgcga tggaggcgcg ccactcgggc gcgcgggcta atcccgtggg
    11041 aactcgagcg gcacgacaaa catccctgcg gtgtattcca cgtggaaata gccgcagcgc
    11101 gcgtaagccg cgatgcgttc gagccgctcc ggtgacggcg aagtgtacgt agcggcagcc
    11161 gtcccgtctg acgattcctc agccgaaaca gccgtatgtc ttgaccattg cttccgcagg
    11221 cttcgcacag gatgccgcag tccgccccag ataagcgtcg ctgtgagtgt ggccatgcga
    11281 cctccgtcga ttcatcgcta taccaatgaa tctagcgatc aggttacgga atcgcaaaga
    11341 agcttttgtg gattggtttt cggaaaccgc cagaaaagcg tgcgaatccc cacacgcacc
    11401 gcggtcgatc attgacctgt gccgacatac acgccgttgg caaacaccgg cgccgcgcca
    11461 tcgcttcgcc gtctttgccg atctataggc caaacccaag ttgcgccttg ccgagcggtg
    11521 tcaggtcgtc ggctgttgcg cgtccggtga aatccacttc gaagtggtca gccgggaaat
    11581 cgggcgggct ttcaccctcg aggaagctcg gcaattgacg tctcaggtaa gcgtcgtttt
    11641 tggcgcacgc gggcgccgag gcgatataca tcacattgct ataacccgcg cccttatgct
    11701 cgtcctcgac ggcatggatg acatcgctgt gccagaacac cgtgtcgccc gcttccattt
    11761 tgggaatcga cgctagtgcc tcgaacaatg gcgcatgaaa ttcccgcttg atcgacagcg
    11821 cccgcccggg cattgctccg cataggtcgt cctccgcgac gtcgtcctga agcgcgcgca
    11881 gcaggatgta caccatcgag ttggcgatcg gaacgagttg cagcgtgccg tcgcccgggc
    11941 cttgcggagt caacgcagtc cagccctgga acgtgcggaa catcgaacag acggccggcg
    12001 acgcgatctc ctccacgtca ggccggaaag ccgcatcgaa cggatcgtag tcgcgccagt
    12061 tccccgcaaa cacgtggcgg tacaccttgc ggaaattgct ttcgatccag cgctcgacgg
    12121 agccgccgtc gcaatgcgcc gacaagccga gcgactcgga ccctggcggg cggcggcgca
    12181 gacgatcggc atagacaggc acacgcgccg gatcgaagtg cacgcggcct tcgctctcat
    12241 tgcgccatag tctgttcaga aacacacgcg ccgctgtcag cgactccgac tgacgggcca
    12301 gtacttgtgg cttcgaccag tacacgccgt agatttgcgg cttgctggac gccaactggc
    12361 caaagtattt gtcttcggcg cggttttcga ggcgcttgtc gagatgattg cgctcgacat
    12421 agccggcaat ttcctcatcc caactttgca ccagcgcacg aggaaaaaca ttgcgaatca
    12481 cgcaggcgcc gcgcgccttg acgagcgcga tctggtccgc ggtgacgcgt tgttcggtga
    12541 tgtcggaaaa ctgaatctcg ggtatgacgt cttccccgcg atcgcgttgc ccggcgattc
    12601 gcttcgcctc ggcgctgatc gcttcttcga cttcggcaaa gacctcccgg tagtttggca
    12661 acgccgcgcg cagcgcgctt ttcgcctgcc ggatcgcgct cggcaaatct tcgattttca
    12721 acgccatgat tgtctccgtc tggactgggt atgcccgagg atatgacgtg catgcgcgct
    12781 ctggtgatac tattcaggcg atttatttgc gttttcggcc atgaaaccag cctatgagca
    12841 tgtcgagttc ggtgaggact gttcgctgcg ggtttaccat cggcgcctcc cccgtattcc
    12901 gttcgagtgg catcaccatc cggagtacga gttaacgcta accatgaaca gtcatggcaa
    12961 gcgctacatc ggcgactctg tcaacgacta tgcggctgag gatctcgtcc tcgtaccgcc
    13021 ggatcttcct cacacctggg cgtcgaatcg ttcgatcgaa ccgtcctctc cacaagtcgc
    13081 catcgttatc tggttcgacg gcgattgggc gcggcgtatg gcggattgtt gccccgaata
    13141 tgaaccgctg cgcaagctgt tgcgcagggc cgcgtgtggg ctggcgttcg agccgcctgc
    13201 cggccaatgg atgcgcggcc agctggattc gctactatcg agcggagcac gcgagcgcct
    13261 tggcgctgcc ctcgatatct tgtgcac
//
LOCUS       DATASH010000030         3528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2180047,
            whole genome shotgun sequence.
ACCESSION   DATASH010000030 DATASH010000000
VERSION     DATASH010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2180047"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..156
                     /locus_tag="VHV99_00955"
     CDS             <1..156
                     /locus_tag="VHV99_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3634061.1"
                     /translation="FGLAVTFVALFQGYEAKPTPEGVSRATTRTVVYASLAVLGLDFL
                     LTALMFS"
     gene            248..811
                     /gene="mlaD"
                     /locus_tag="VHV99_00960"
     CDS             248..811
                     /gene="mlaD"
                     /locus_tag="VHV99_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006052274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEX3634062.1"
                     /translation="MKKTALDFWVGLFVVLGFVALLFLALKAGNMSSLSFQATYPVKL
                     KFDNIGGLKARAPVKSAGVTVGRVDSIGFDSNAYQAVVTIDIDKQYPFPKDTSAKILT
                     SGLLGEQYIGLEPGGDSEMLKAGDTISMTQSAIVLENLIGQFLYSKAADSGASKPGAG
                     STAPAAGPATAPAPVAPTLPASGAAGQ"
     gene            823..1899
                     /locus_tag="VHV99_00965"
                     /pseudo
     CDS             823..1899
                     /locus_tag="VHV99_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433816.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
     gene            2018..2650
                     /locus_tag="VHV99_00970"
     CDS             2018..2650
                     /locus_tag="VHV99_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006052276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3634063.1"
                     /translation="MKKLFLIPLFAALFSFASAGASAQTVDANSPDGLVKTVTQQVID
                     AVRADKSIQQGDISHITQLVNQKILPYTDFRRTTQLAMGRNWRTATPEQQNAVVEQFK
                     MLLIRTYSGALAQVRDQQIQYKPFRMNPDDTDTVVRSVVMNNGSPIELDYRLYKTPNG
                     WRVYDINVLGAWLIQAYQQQFNEQIQQKGVDGLIQFLTQRNQQLAAGKQS"
     gene            2647..2940
                     /locus_tag="VHV99_00975"
     CDS             2647..2940
                     /locus_tag="VHV99_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX3634064.1"
                     /translation="MSEMLNAVASRFECGATLNHESAKAALAAGLQRIAAGANGVDCA
                     SLAQFDSSALAVLLAWERAAQARGVKFEIVNLPAGLASLAQAYGVDTLLTTRH"
     gene            3110..3528
                     /locus_tag="VHV99_00980"
                     /pseudo
     CDS             3110..3528
                     /locus_tag="VHV99_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440405.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
BASE COUNT          678 a         1126 c         1089 g          635 t
ORIGIN      
        1 ttcggcctcg cagtgacctt cgtcgcgttg tttcagggct acgaagccaa gccgacgccg
       61 gaaggcgtgt cgcgggcgac gaccaggacc gtcgtctacg cgtcgctggc ggtgctcggc
      121 ctcgattttc tgctgaccgc actgatgttc agctaagact gcgctgcacg cttgccgtca
      181 ttgggcgcgc ctcgggttgc gcgtgacgga ggcgcgagcg gcgtggcgga ttcactttgg
      241 gatgacgatg aaaaagactg ctctcgactt ctgggtcggc ctgttcgtgg tgttgggttt
      301 cgtggcgttg ctctttctcg cgctgaaggc cggcaacatg agctcgttgt cgtttcaggc
      361 aacatacccg gtcaagctca agttcgacaa tatcggtggc ctgaaggcgc gcgcgccggt
      421 gaagagcgcg ggcgtgacgg tcggccgggt cgattcgatc ggcttcgaca gcaatgccta
      481 tcaggctgtc gtcacgatcg atatcgacaa gcaatatccg tttccgaaag acacctcggc
      541 gaagatcctg acctcgggtc tgctgggtga gcaatacatc gggctcgaac ccggtggcga
      601 cagcgagatg cttaaggcgg gtgacacgat ttcgatgacg caatcggcga tcgtgctgga
      661 aaacctgatc ggacaattcc tgtatagcaa ggccgcggac tcgggggcat ccaagccggg
      721 cgcgggttcg actgccccgg cggccggccc cgccacggcg cccgcgccgg tggcgccgac
      781 tctgcccgcc tccggcgcgg ccggtcaata aggagaacaa aaatgcagac cataggcacg
      841 aagggtatgc gcgccgtcca gatggcgaca ttcgcgctcg cggccgctac cttggtcggc
      901 tgctcgaccg tgcagacgcc gaccaagggc gacccgctcg aaggcctgaa ccgcaccatc
      961 ttcaccgtca acgacaagct cgaccagtac gcgctgaagc cggtcgcgaa gggctatgtg
     1021 tttgtgacgc cgcaaccggt gcgcgacagt gtcaccaact tcttctcgaa catcggcgac
     1081 gtctacatcg cggcgaacaa cctgctgcaa ttgaagatca ccgatggcgt ccaggacatc
     1141 atgcgcatcg tgatcaacac ggtgttcggc gtcggcggtc tgttcgacgt ggcgacgctc
     1201 gccaagctgc ccaaacacga caacgatctc ggcctgacgc ttggccacta cggcgtgccg
     1261 gcgggtccat atctcgtgct gccgctgttc gggccgagca cggtgcgcga cgcggtcggc
     1321 tcggtcggca actactatgt gaatccgctc agctacattc atccggatgg tctgagctgg
     1381 gcgttgtacg gcctgaacgt catcaacacg cgtgcgaatt tgctgggcgc gagcgacgtg
     1441 ctgtaaggcg ccgcgctcga caaatactcg ttcgtgcgta acgcctatct gcaacgccgc
     1501 cagtatctgc tgtcggacgg caagcaagcg caggcgctgc cgaactacgg cgatgaagcg
     1561 ccgctgccga agtacgacga cgtcgatggc ggcgcggctg cggcgccggc cggtgccgtg
     1621 agcggcacgg ccggtgcggc tggtgcggcg ggcacgcaag gtgctgctgc gaaggcggcc
     1681 gctgcttccg gtacggcagc agcgacaccg ccgcaagccg cttcggcagc gggtgcaacc
     1741 gcaggagcga ccggcacggc aggtgccccg gaagccgcct ccggcagcgc cgaatcgccg
     1801 ccgctcgatc tgaacggcgg tccggaaacg acgcagatcc ccgccggcca gctggtcccg
     1861 ccgacacgtt tcaactttcc gtcattcaaa ttgcattgat cgtgcagccg taacacctcg
     1921 aaacgactct cgcagtttgc gcatggattg ctgctgaact cgcgtgctaa tgtcggctca
     1981 aacggttgca ctatttttaa ggcaagcaaa gctcgatatg aaaaaactat tcctgattcc
     2041 gttgttcgct gcattgtttt ctttcgccag tgccggtgca tcggcacaaa cagtcgacgc
     2101 caattcaccg gacggcctgg tcaagacggt cacccagcag gtgatcgatg cggtgcgcgc
     2161 cgacaaatcg atccagcaag gcgacatctc gcacatcacc cagctcgtca accaaaagat
     2221 tctgccgtac accgatttcc gccgcaccac gcaactggca atgggccgca actggcgcac
     2281 cgctacgcct gagcagcaaa acgccgtggt cgagcagttc aagatgttgc tgatccggac
     2341 gtactcgggc gcgctcgccc aggtccgcga ccagcagatc cagtacaagc cgttccgcat
     2401 gaacccggac gacaccgaca cagtggtgcg ctcggtggtg atgaacaacg gctcgccgat
     2461 cgaactcgac taccgcctgt acaagacgcc gaacggctgg cgcgtgtatg acatcaacgt
     2521 actcggcgcg tggctgatcc aggcgtacca gcaacagttc aacgagcaga tccagcagaa
     2581 gggcgtggac gggctgatcc agtttctcac gcagcgcaat cagcaactgg ctgcgggcaa
     2641 gcagtcgtga gcgagatgct gaacgccgtc gccagccgct tcgaatgcgg cgcgacgctg
     2701 aaccacgaga gcgcgaaagc cgcgctcgcg gcgggtctgc agcgcatcgc cgcgggcgcg
     2761 aacggcgtgg attgcgcgtc gctcgcgcaa ttcgattcgt ccgcgctggc cgttctgctc
     2821 gcgtgggagc gtgccgccca ggcacgcggc gtcaagttcg agatagtcaa tctgccggct
     2881 ggtctcgcca gcctcgcaca agcctacggc gtcgataccc ttctcacgac gcgacattga
     2941 cgctcccata gcgtcacttc caatcgcccc gcctgagccg gggcttctga tttttgccct
     3001 ataatcaaac gttttttggg gcagaaaacc ggccccaatt tcgtcccttc cagcactttt
     3061 gcgcccctca gggctccttt aggcgccgcg acccacgcgg cccacagtca tgtcagccat
     3121 agaaattcgt aacgtcaaga agcgctacaa agacttgcag gcgctcaagg gcgtcagtct
     3181 cacggtggaa gaaggtgagt tcttcggact gctcggtccg aacggcgcgg gcaagaccac
     3241 gctcatcagc atcctcgcgg gtctcgcacg cgccgatgaa ggcagcatcg cggtacgcgg
     3301 ccatgatgtc gtcagcgact tccgcgacgc gcgccgcgcg ctcggcgtgg tgccgcaaga
     3361 gctcgtgttc gaccctttct tcaccgtgcg cgaaaccttg cgcatccagt ccggctacta
     3421 gggcctgcgc aataacgacg cgtggatcga cgagatcatg gccaatctcg acctcaccga
     3481 caaagccgac gccaacatgc gtgcgctgtc gggcggcatg aagcgccg
//
LOCUS       DATASH010000031         2147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2772903,
            whole genome shotgun sequence.
ACCESSION   DATASH010000031 DATASH010000000
VERSION     DATASH010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2772903"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="VHV99_00985"
     CDS             complement(<1..823)
                     /locus_tag="VHV99_00985"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3634065.1"
                     /translation="MVANPTLLFPNRTEQVEPATSRKLLAVEHVNLEYRTRERIVRAT
                     HDVSFDVYGGDRFVLLGPSGCGKSTLLKAVAGFIEPTSGSISLDGQTVRGPGADRIVV
                     FQEFDQLPPWKTVLQNVAFPLRVAKKFSRAEANERALHYLEKVGLASFANAYPHTLSG
                     GMKQRVAIARALAMQPRVLLMDEPFAALDALTRRKMQEELLRLWEEVNFTLLFVTHSI
                     EEALVVGNRILLLSPHPGRVRAELNSHQHSQDSFGRSDFQRSVARIHHLLFEETEA"
     gene            complement(894..1946)
                     /locus_tag="VHV99_00990"
     CDS             complement(894..1946)
                     /locus_tag="VHV99_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3634066.1"
                     /translation="MLAMSRPTALRSSFARRLTATLLTLSMGVAGLGATAPAHAEGQI
                     RVAEQFGVVYLLLNVARDQQFVEKEGRKQGLDIKVDWVKLSGGAAVNDALLSGAVDIA
                     GAGVGPLLTIWDRTHGKQNVKGVASLGNLPYYLVSNNPNIKTIADFTGKDRIAVPAVD
                     VSVQSRVLQYAAAKRWGDKDYNRLDKFTQALPHPDAAAAIIAGGTEISGHFGNPPFQE
                     QELAGNPKAHIVLNSYDVLGGPSSATVLYATEKFRDNNPKTYRAFVDAMADAARFVTA
                     NPDAAADIYIRVNQSKIDRNLLLKVIKDPQVQFKVAPQNTLGLAQFMHRVGVIRNEPK
                     SWKDYFFDDPATAAGS"
     gene            complement(1994..>2147)
                     /locus_tag="VHV99_00995"
     CDS             complement(1994..>2147)
                     /locus_tag="VHV99_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011882254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX3634067.1"
                     /translation="VRPEHLYRHQWAEHDMVFWDNRSLMHLAAGTPDHLRRKMYRTTI
                     EGDVPF"
BASE COUNT          408 a          677 c          659 g          403 t
ORIGIN      
        1 cggcctccgt ctcttcgaac aacaggtgat gaatacgcgc gacgctgcgc tgaaaatcgc
       61 tgcgcccgaa actgtcctgc gaatgctgat ggctgttgag ttccgcgcgc acacggccgg
      121 gatgcggcga cagcaacaga atgcggttgc ccaccaccag cgcttcttcg atcgaatgcg
      181 tgacgaacag cagcgtgaag ttcacttctt cccacagacg caaaagctct tcctgcatct
      241 tgcgacgcgt aagcgcatcg agcgcggcga agggctcgtc catcagcagc acgcgcggct
      301 gcatcgccag cgcacgggca atcgcaacgc gctgcttcat gccgcccgac aacgtatgcg
      361 gataggcgtt ggcaaaagac gcaagcccca ccttctccag ataatgcagc gcccgttcgt
      421 ttgcttccgc gcgcgagaac ttcttcgcga cccgcagcgg aaaggcgacg ttctgcagca
      481 ccgtcttcca cggcggcaac tgatcgaact cctgaaacac gacaatgcga tccgcgccgg
      541 gaccgcgcac agtctgaccg tcgagcgaga tactgccgga ggtcggctcg ataaagcccg
      601 ccacggcttt gagcagggtc gacttgccgc aacccgacgg tcccagcagc acgaaacggt
      661 cgccgccgta gacgtcgaaa ctgacgtcat gggtcgcgcg aacgatccgc tcgcgggtgc
      721 ggtattcgag gttgacgtgt tcgaccgcga gcagtttccg gctcgtcgcc ggctcgactt
      781 gctcagtgcg gttcggaaac agcaaggtag gattggccac catcagatcg ctcccggtaa
      841 gccggccact cgggccgata aagatccaac gaagcgcgga taggcattca tgctcagctt
      901 cccgccgcgg tcgccgggtc gtcgaagaag taatccttcc acgatttcgg ttcgttcctg
      961 atcacgccga cgcgatgcat gaactgcgcg aggcccaacg tgttctgcgg tgcaaccttg
     1021 aactgcacct gcggatcctt gatgaccttc agcagcagat tgcggtcgat cttcgactga
     1081 ttcacgcgga tgtagatatc ggccgcggca tcgggattcg ccgtgacgaa gcgcgcggcg
     1141 tccgccattg cgtcgacgaa ggcgcggtaa gtcttcggat tgttgtcgcg gaatttttcc
     1201 gtcgcgtaca gaacggtcgc cgagctcggg ccgcccagca cgtcgtacga attcagcacg
     1261 atgtgcgcct tcggattacc cgcgagttcc tgttcctgaa acggcggatt gccgaagtgc
     1321 ccgctgattt ccgtgccgcc cgcgatgatc gccgcggccg cgtcgggatg cggcagcgcc
     1381 tgggtgaact tgtcgaggcg gttgtaatcc ttgtcgcccc agcgtttcgc cgccgcgtat
     1441 tgcagcacgc gcgattgcac cgacacgtcg acggccggca ccgcaatgcg gtccttcccg
     1501 gtgaagtcgg cgatcgtctt gatattggga ttgttgctga cgaggtaata cggcagattg
     1561 ccgagcgacg ccacgccctt cacattctgc ttgccatgtg tacggtccca gatcgtcagc
     1621 aaggggccga cgcccgcgcc ggcgatatcc accgcgccgg agagcagcgc gtcgttgacg
     1681 gcggcgccgc ccgagagctt cacccagtcg accttgatat ccagcccttg cttacgccct
     1741 tctttctcga cgaactgctg gtcgcgcgcc acgttcaaca gcagatagac gacgccgaac
     1801 tgttcggcca cgcggatctg cccttcggca tgcgcgggcg cggtcgcgcc gaggccggcc
     1861 acacccatcg acagggttag cagcgtcgct gtcaaacggc gagcgaaaga cgaccgcaaa
     1921 gcggtcgggc gagacatggc gagcattgga actccggtca gcgtgtgatg gtgtaaagcg
     1981 gggaacgttg ccgtcagaac ggcacgtcgc cttcaatggt tgtgcgatac attttgcggc
     2041 gcagatgatc cggcgtaccg gctgcgagat gcatcaatga gcggttgtcc cagaacacca
     2101 tgtcgtgttc ggcccactga tgtcggtaca gatgttcggg gcgcacg
//
LOCUS       DATASH010000032         2637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4949412,
            whole genome shotgun sequence.
ACCESSION   DATASH010000032 DATASH010000000
VERSION     DATASH010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4949412"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VHV99_01000"
     CDS             <1..223
                     /locus_tag="VHV99_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007740894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEX3634068.1"
                     /translation="ENSHQPTRRRERQMCGFRDARRTQAFLSCFGLIRQHFALPRHQM
                     NAACHRAVLNERFATWHDWTVTGAVEKVT"
     gene            563..787
                     /locus_tag="VHV99_01005"
     CDS             563..787
                     /locus_tag="VHV99_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016354434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634069.1"
                     /translation="MVLYHRGAAIQPSVTREGNAFVARAVILEEDGEATSPGMLGQFA
                     SQNSAYEFALRSATEVAPTRINSCAAARLP"
     gene            complement(709..1038)
                     /locus_tag="VHV99_01010"
                     /pseudo
     CDS             complement(709..1038)
                     /locus_tag="VHV99_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876239.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
     gene            1056..1529
                     /locus_tag="VHV99_01015"
     CDS             1056..1529
                     /locus_tag="VHV99_01015"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEX3634070.1"
                     /translation="MREMIETFAIRKFCRGGAPAIDLIRLLDALDAIWQVPLDERFAD
                     GREVATLDKAFHQTLVSAAGNDELTAALQCVTDRIRVVRRLDLVYGNCVDETYVEHAA
                     ILRAIRAGRTDEAVTGAASHIRVNQAEVRKLTVPRLHSVRTTGVRRAAPDAPVKG"
     gene            1779..>2637
                     /locus_tag="VHV99_01020"
     CDS             1779..>2637
                     /locus_tag="VHV99_01020"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX3634071.1"
                     /translation="MSAEFLNPTRGIRGMAVAPHALASQSALAILREGGNAIEAMIAA
                     AATIAVVYPHMNSIGGDGFWLISRPGEAPVGIEACGAAAAAASIDAYRALGVQSIPFR
                     GPLAAITVAGTVSGWALAHRWSTDTLGGALPVARLLEDAIAYAGGGIPVTRSQSECVA
                     LKRPELEAVAGFAETFLTSDNPPGVPAVGDRFVQPRLAATLERLAHAGLDDFYRGDLA
                     RSIATDLRALESPLSLTDLEQHHARLRTPLALTHSLGTVYNMPPPTQGLVSLQILGVL
                     DRVLRDGMDP"
BASE COUNT          505 a          833 c          776 g          523 t
ORIGIN      
        1 cgagaacagc caccagccga cccgcaggcg cgaacgccag atgtgcggct ttcgcgatgc
       61 gcgtcgcacg caggcgtttc tttcatgctt tggcctgatc cggcagcact tcgcgttgcc
      121 cagacatcaa atgaacgcgg cttgtcatcg tgctgtgctc aatgaacgct tcgccacatg
      181 gcatgattgg actgtcactg gcgcagtcga gaaagttacc tgattaggat aactactctt
      241 gagcttcaca ggtagtctac gccccgccaa tttgacagag ccgctcccac gcagcacatg
      301 ggatcgcgta gtcacgcgct acaacagcgc cacggatctg atcgaagcag gagtcggttt
      361 gcgcttttga gcgtatcacc aacgatcatt gttttcgctg ttgccagcgc gtggatcgag
      421 atcaaactac tgtttgattc gcgcctgatt cgattcatag taattatgcg gctaacgaga
      481 aaggataacg tttccgtcga caaaactagc acagtaagat ggcactcgcg gttcccataa
      541 gacaactgag agaggatttg agatggtcct ataccaccga ggtgcggcga tacaaccttc
      601 cgtcacaaga gaaggtaacg ctttcgtagc gcgcgctgtc atcttggaag aggatggcga
      661 ggctacttca ccgggaatgc taggccagtt cgcgagccaa aattccgctt acgagtttgc
      721 ccttcggagt gctacagagg tggcaccgac tcgtatcaac tcttgcgccg cggcacggtt
      781 gccctgagcg atcacagtct gcatagcggg atcgccccgg gcatccgtat gattcggatt
      841 agcccccctt cttcgccaac tctggaagca actggaagcg tccaaaaagc gcggcaaagg
      901 caagagccgt ttcgcaggca ttgttcgttt ggttgaagtt gcattgcgtc cccatcaaag
      961 ttcgcgcgat atcagtctgc cctttgtata tcgccccatc aacgcggtgt tgccgtttct
     1021 gtcgccaacg catgcgtttc gacaatctct acgaaatgcg cgagatgatc gagacgtttg
     1081 cgatccgcaa gttctgccgc ggcggcgcgc cggccatcga tttgatccgc ctgctcgacg
     1141 cgctcgacgc catctggcaa gtgccgctcg acgagcgctt cgccgacggc cgcgaagtgg
     1201 cgacgctcga taaagcgttt caccagacac tcgtgtcggc ggccggcaac gacgaactca
     1261 ccgccgctct gcaatgcgtc acggaccgca ttcgcgtcgt gcggcgactc gatcttgtct
     1321 atggcaactg cgtcgacgaa acgtatgtcg aacatgccgc catcctgcgt gcgattcgtg
     1381 ccggccgtac ggacgaagca gtcaccggcg ccgcgagtca catccgcgta aatcaggccg
     1441 aggtccgcaa actgaccgtg cctcgtctcc atagcgtgcg caccaccggg gttcggcgcg
     1501 ctgcgccgga cgcaccagtt aagggctaac gcaccatctg cactcattgt tgcgcgtcgg
     1561 tcgccggatt atttcgctgg ttcgcccgat agcccaaacc ggccaccaag cgcgtagtca
     1621 taagggtttc ccctaaaaaa agcgcaatcc acaaggatca ggcacgattc tcgcttttgt
     1681 cgacgttgtt gtatacaagc atcgagccca ctgtctgacg aacccgggtg gttcgcaatc
     1741 gcatgggccg ctcagttgat cgattaacgc agggaaacat gtcggcagaa tttttgaacc
     1801 ccacgcgggg catccgcgga atggcggtgg cacctcacgc gctggcttcg caaagcgcgc
     1861 tcgcgatcct gcgcgaaggc ggcaatgcca tcgaggcgat gattgcagcc gccgcgacca
     1921 tcgcggtggt gtatccgcat atgaacagca tcggcggcga cggcttctgg ctgatctcgc
     1981 ggccgggcga agcgcccgtc ggcatcgagg cgtgcggcgc ggcggcagcg gcggcctcca
     2041 tcgatgccta tcgcgcgctc ggcgtgcaat ccattccgtt tcgcggcccg ctcgcggcca
     2101 ttactgtggc cggcaccgtg tcgggctggg cgttggccca tcgctggtcc actgacacgc
     2161 tcggcggcgc cctcccggtc gcgcgcctgc tggaagacgc gatcgcctac gcaggaggcg
     2221 gtattccggt cacgcgcagc cagtccgaat gcgtcgcgct caagcggcct gaactcgaag
     2281 cggtcgcggg gttcgccgag acgtttttga cgagcgacaa cccgccgggt gtgccggccg
     2341 tcggcgatcg ctttgtgcag ccacgcctcg ccgcaacgct cgagcgtctc gcccacgcgg
     2401 gtctggacga cttctaccgg ggcgatctcg cacgcagcat cgcgaccgac ctgcgggcgc
     2461 tcgaaagccc actctcgctg accgacctcg aacagcatca cgcgcggttg cgcacgccgc
     2521 tcgcgctgac gcattcgctc ggcacggtct acaacatgcc gccgcccacg caagggctgg
     2581 tttcactgca gattctcggt gtgctcgacc gggtcttgcg cgacggcatg gatccgc
//
LOCUS       DATASH010000033        29842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4751667,
            whole genome shotgun sequence.
ACCESSION   DATASH010000033 DATASH010000000
VERSION     DATASH010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 29842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29842
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4751667"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VHV99_01025"
     CDS             <1..214
                     /locus_tag="VHV99_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634072.1"
                     /translation="WERPMQVEGKTATSVIYTYKIDPSPWAKDADAQRVFPMIKRVIE
                     GAGTLQLREGVHLTSNGWVADEVFVR"
     gene            complement(277..1167)
                     /locus_tag="VHV99_01030"
     CDS             complement(277..1167)
                     /locus_tag="VHV99_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEX3634073.1"
                     /translation="MSITVNFPAEVRDWIAHNLTRGVPPQAIVRELVNQKNAVELATA
                     MVDAVSNSFLYGIPLPGETLDIGGAPVTYEPEPLRVPDGPLIRLGERKTRVISRMQRP
                     AAVLLDDFLSANECEQLIALARPRLNHSTVVDPVTGRNVVAGHRSSEGMFFRLAETPL
                     IARLEARIAELTGLPAENGEGLQLLHYEAGAESTPHVDYLIPGNPANQESITRSGQRV
                     GTLLMYLNDVEGGGETVFPQLGWSVVPQRGQALYFEYGNRFGLSDPSSLHASTPLRTG
                     EKWVATKWIRTRRFVPRDQA"
     gene            complement(1260..2042)
                     /locus_tag="VHV99_01035"
     CDS             complement(1260..2042)
                     /locus_tag="VHV99_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX3634074.1"
                     /translation="MPGVTERTMAVLEFLATQMEGTPLALISDQLEIPRSACHRLLVD
                     LKQCGYIRQLREHGDYVLTTKLAGLGLSYLATSGIIDIAQTMLDRLAETSGELVRLAI
                     VDGDRLTWVAKSQGALKGLRYDPDMGMDTILSCSATGHAWMMTMTDERALELVTRQGF
                     GKPKQYGPNAPTTVKALLGFVDAARARGYATIYEVFAPGMTAMAAPVQRRGYPAIGVI
                     SIAGPAVRLTEKRMTALGPALVAAANELAAASLASPLFDRVR"
     gene            2270..3637
                     /locus_tag="VHV99_01040"
     CDS             2270..3637
                     /locus_tag="VHV99_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007736281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3634075.1"
                     /translation="MDQQLQQPAHQAMRTRAVTAAAIGTALEWFDFTLYGALAATVLP
                     KLFFPSMDSTSALLASLATFGVGLAARPLGAIICGYLGDKLGRRNLMLGTVTVMGVCS
                     MLMGLLPTYASIGVWAPLLLVVLRILQGFALGGESTGAQLMAIEHASPDRRGKYSGLL
                     GLCSPLSQIMANGVLLALSSAMSAQAFDEYGWRIPFVLSFILVIVGIYIRLRVSETPA
                     FVALRKTEVAHVGSPLRDALRLHWRTVLRWMLFFCGPAAIFYLIVVFSLSYVTKTLGV
                     PKQTGFLLLMGANVCAIVGALAGGMLSDRIGRKKALAIGSIATLLMLFVYFQILDTKS
                     FVPMLAAMGFFLGFTQFQSGIQPVAFAEAFPTNVRYSGSALAYTGANLIAGGPMPVLA
                     VWLFAVCNGSPWGVVAVCVTFNVISLITILTAPETLGIDMNRVDSTEQVMGSSEHVPT
                     RSHSH"
     gene            3655..4104
                     /locus_tag="VHV99_01045"
     CDS             3655..4104
                     /locus_tag="VHV99_01045"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEX3634076.1"
                     /translation="MKPLVYVLNGSNLNMLGKREPHLYGTTTLAQVKERTEALAAELD
                     LECHFRQTNHEGVMVDWLQEAFEKDAAVVINPAGFSFASIPVLDAVKLIRQPVVEVHI
                     TNIHQRGEQYRHSLISLAAGGVICGLGVDGYLLAVRAVAGAIQRAKV"
     gene            complement(4162..6399)
                     /locus_tag="VHV99_01050"
     CDS             complement(4162..6399)
                     /locus_tag="VHV99_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011494311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX3634077.1"
                     /translation="MPTLPDSVLSVIGRPHARVDGPLKVSGSATYTSDVDLPGLLIAV
                     PVCSTIASGRLTSLDIADAEAMPGIHAVLHRGNIGRFYRISGNSLETGYVDEARPPFE
                     DDIIRYYGQYIAVVIADTFEAASAASAAIKAGYEITPHDVRGELEPDDTPKVESERGD
                     PDGAFDAASAKLDATYVTPTETHNPIELHATIAQWDGESYTFYETTQSISAHLGTLSQ
                     MLGVPKEKIRVISRYLGSGFGGKLWMWPHSLLAAAAARQTGRPVKLVVSRKMMFQNVG
                     HRPVTQQRVRLSAERDGSLTSLQHDYINHTAIADEYQEGCGEVTPLIYSTPNLRVTSA
                     LARRNVGSPTSMRGPGAMPGLYAIESAMNELAQVLHMDPVELRLRNEPRVDESTGLPF
                     SSRHLVECLRTGAERFGWSQRTPGVGSMHRDGLTLGWGVGACVWPGMRFSAAADVDLR
                     VDGSARVVCGTQDIGTGTYTVLAELVAEETGIPLERIEVGLGDSALPPGPISGGSAAT
                     ASVIPAVLDAVRAAIKLVLSRAAAVEGSPFKGAEEEGLAFSAAHVHRKGEAPERGVPF
                     GQILEAAKMHAASGSGSAKGGFDDPLKKRFSIYSYGAHFAEVTWQPEIAQLRVSRVVT
                     VIDGGRIINPCAARNQIEGAVVMGVGMALFEHTIYDRQTGAPINSNLADYIIASNADT
                     PELDVTFLDHPDTVFNELGARGIAEIGLAGIAAAITEATHHATGVRVRKLPIMIEDLL
                     AAEIA"
     gene            complement(6424..7428)
                     /locus_tag="VHV99_01055"
     CDS             complement(6424..7428)
                     /locus_tag="VHV99_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014252693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX3634078.1"
                     /translation="MEMFQLSRADGVPDAIAAGAAGVTAQQGAQVRFLAGGTTLLDLM
                     KLNVERPVRVIDISRLPLDRIEALPGGGVKIGATARNADLALHPLIREPYAVLSQALL
                     SGASAQLRNMATTGGNLLQRTRCVYFRDTAMPCNKREPGSGCPAIDGFNRTLAILGTS
                     DACIATNPSDMNVALTALDATIHVEGTDGARSIAIDDFFLLPGATPQRENVLGAGDLI
                     THVTLPPLPAGTRSVYLKLRDRASYEFALASAAVVVTLDGGRIGHARVALGGVGTRPW
                     HALEAQALLQGATPGAAIFRKAADAALANAKPQSQNGFKVELARRCIIHALTQATATT
                     "
     gene            complement(7440..7994)
                     /locus_tag="VHV99_01060"
     CDS             complement(7440..7994)
                     /locus_tag="VHV99_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011494309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX3634079.1"
                     /translation="MMQQTASPIHTPQQSVERDANRTVLQPSANTTMSVELTVNGHEY
                     ELSLDPRTTLLDALREHLHLTGTKKGCDHGQCGACTVHVNGRRVNACLSFAGHHGGDE
                     ITTIEGIGQPDALHPMQAAFVECDGYQCGYCTSGQIMSAVALLDEAVGPGNAEVREAM
                     SGNLCRCGAYQNIVAAIQRVRGQS"
     gene            complement(8190..9305)
                     /locus_tag="VHV99_01065"
     CDS             complement(8190..9305)
                     /locus_tag="VHV99_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012406328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX3634080.1"
                     /translation="MDALSDVLRLVRLGGAVYLNGEFTAPWCVAGEATPALYATFLPR
                     AERIVSYHLITEGSCCARLVGDPTCMIRVDAGELLVVPQGEAHLLGSSLELSPAPSDE
                     MLSQFLKTVPGEVMELSYGGGGARTRLICGFLACDDTLTNPVLSALPRIFKVDMRNDP
                     QSAWLESSLKLAAVEAGEGRVGSAIVLARLSELLFVEAVRRCIDALPDDRKGWLAGVG
                     DRYVGRALALLHAQPAHGWTVDELARGAGLSRSALAQRFTQLLGLPPMQYLARWRLQI
                     AAQELRNGTRSLAAVAERVGYDSEAAFNRAFKREFGMPPASWRRSHGITDGRDSVSVA
                     ENFSDLSVDPFATGAVPSTQTQPVPLTKVLPAGRGAH"
     gene            9455..9889
                     /locus_tag="VHV99_01070"
     CDS             9455..9889
                     /locus_tag="VHV99_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007747406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX3634081.1"
                     /translation="MSHANTYRGQCFCGAVQFTVSGEPAGMGYCHCDSCRRWSGGPVN
                     AFTLWKPESLSVTRGADDIGTYNKTPHSYRKWCRSCGGHLFTEHPGLGLIDVYAAVMT
                     DFPYEAGVHVHYPESVLPMNDGLPKLRDLPREMGGSGISVGE"
     gene            10029..10862
                     /locus_tag="VHV99_01075"
     CDS             10029..10862
                     /locus_tag="VHV99_01075"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3634082.1"
                     /translation="MSAVDSSFSPVADFTAVKVRQQAAWSTGNYAVVGTTLQIVGENL
                     CEALDLRAGSRVLDVAAGNGNATLAAARRCCDVISTDYVASLLESGRARAQAEGLPVQ
                     FQEADAEALPYADASFDVVMSTFGVMFTPDQEKAAAELARVCKPGGRIGLANWTPESF
                     IGQVFKTIGKHITPPAGVVSPALWGTKERLHALFGANARQIVATSRFFTFRYRSPAHF
                     VDVFRTYYGPMNKAFAALEGDRQAALLGDLMTLIESRNRSGDSTVVLPSEYLEVVVER
                     S"
     gene            10859..11035
                     /locus_tag="VHV99_01080"
     CDS             10859..11035
                     /locus_tag="VHV99_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634083.1"
                     /translation="MNKVRREVAALVSAGSRLWCLACLIGLRHGSMLVAVPLCSSVCF
                     FSVRFALSALGLSV"
     gene            11442..11579
                     /locus_tag="VHV99_01085"
     CDS             11442..11579
                     /locus_tag="VHV99_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3563 family protein"
                     /protein_id="HEX3634084.1"
                     /translation="MFIYLFLLLRKALERAEHSRRDAYLASAVDIGELERRMHSIDFD
                     G"
     gene            11744..12553
                     /locus_tag="VHV99_01090"
     CDS             11744..12553
                     /locus_tag="VHV99_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007747413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3634085.1"
                     /translation="MNSTIASQVISPATTVIALHCSGSGAAQWRKLGEALGSRHALVA
                     PEHYGCDSTGPWSGERAFTLADEAARTIGIIDAARGKVHLVGHSYGGGVALRAAVERP
                     GRIASLTLYEPSAFHLLKVMGAHGAYALAEIIAISKRTADGVSCGDYRGAAASFVDYW
                     GGRGSWEALRPSVQAALTRWAPKAPLDFRALLDERTPASAYRSLRVPTLIMRGQHAPI
                     PTRAIAERLPMLLPAARLAIVEGAGHMGPLTHADKVNATIVGHITESEAAA"
     gene            complement(12615..14939)
                     /locus_tag="VHV99_01095"
     CDS             complement(12615..14939)
                     /locus_tag="VHV99_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008348403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEX3634086.1"
                     /translation="MALADRVKAAACSPAWPEQTGAPFIGKSGIWLNGVTYWVLYLLA
                     RLLSFEALMSTDAETITDELIAGIADRMVEEGRKVSPVTIWPEVPGGSMVAIAASLQR
                     WREARQPDVPHARIQTALPEHVAETMMSATDRLWMAALGEADKAVSQHLSALNQHIDT
                     ARAERDEALAEYQKMLEEFESSRERQIGLTSSLSASEEAATRLSAELSAATGRADAAE
                     ARAEELAQRVSVEEAALEHTKAELDEERKAREALAAAVSNQKDEIARMTQSLDAAQQE
                     ITSLGYDCQAKSAETDRALEEAGEASARAEAATAQSNESRARVAALEGELGEAHVSLE
                     AERRASAARSEEASLQLEELQRVTRELEAAREQVSAMTEAKAAVSAELAQVSQDASAA
                     SAELARVSQEASAAKERAETAEQHSAALQQRIAEIEQAKSAEAERWAQESSVVSSRAE
                     AAAAQANESLARIAALEAELNETRIRLEAELIETRTGLEAELKETRTALEAERRTSTA
                     RGEEASVQLNKLQRVAREREEAREQVSAMSEAKAAAIAELDKVSQDVAAVSAELFKAS
                     QDASAAKERAQAAEQHAATLQQRLAEVEQANSAEAERWTQEVSAASSRAEAAAAEAHE
                     SVARLAALEAERDEARAALAAARQAARSEESATAEEIAELQRQISAQAKAHEKAFNEL
                     RTIAEQWVAHAKDLKARLGSAQEKLLFIDSRSTGEVALIRKLSSELERLKPDSELISR
                     DTQQKLIGATMAERLSQKGYRYDPATAVMSKVER"
     gene            14992..15378
                     /locus_tag="VHV99_01100"
     CDS             14992..15378
                     /locus_tag="VHV99_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634087.1"
                     /translation="MPSAIRNSLIWIFDAFERDSTYVHKRMFGSDAAYIDGLLCLIAA
                     DREKPWNGLLVCTSQDRHAALIEEVPALRPHPVLGKWLYVQQDDPAFEPSVERMTALV
                     LARDPRVGVEPKARKRGGKSALPREA"
     gene            complement(15565..16608)
                     /locus_tag="VHV99_01105"
     CDS             complement(15565..16608)
                     /locus_tag="VHV99_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3634088.1"
                     /translation="MPDQINIRRRRLLGTTVAGISLMELGLSGLAQAQSNSTTSNTKA
                     TGQVASFGAIRQVNAGALSIGYAEAGPRNGPVVILLHGWPYDIYSFAEVTPLLAAAGY
                     RVIVPYLRGYGSTRFLSADTPRNGQQAVVAVDIIALMDALKIDQAILGGFDWGARTVN
                     IIAALWPQRCKAMVSVSGYLIGSQEANRAPLPPKAELAWWYQFYFATERGQAGYEANR
                     HDFNKLIWRLASPRWNFDDATFDRSAESFNNPDHVAVVIHNYRWRLGLVKGEPQYDEL
                     EARLAKAPTISVPTITMEGDANGAPRPEPAAYAKKFTGKYAHRNIDGGIGHNLPQEAP
                     KAFADAIIGVVRL"
     gene            complement(16707..18053)
                     /locus_tag="VHV99_01110"
     CDS             complement(16707..18053)
                     /locus_tag="VHV99_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013342602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX3634089.1"
                     /translation="MKPSSLLRWPRSLFARLALILFVSLALVQTLSVWLTMTERDQNL
                     NNVMMGYIEREVTSSVALLDHLPADERAAWLPRLARRTYTFVLGPGVAGAPPDVALSE
                     RVAQSIADGIGRRYPLTVNAVPGDPDRLQVHLQLSDGTPLTIDYRPMPGAPLSPWLSW
                     VLVLQLVVLAACCWLAVRLATRPLSHLARAADTLGPDLKAERLPEDGPDEVARAARAF
                     NAMQDRIRMYMTERLQILAAISHDLQTPITRMRLRVDVMDDSPQGAKLQQDLQEMETM
                     VKEGVTYARTMHGATEAPLRIDPDALFDSLVFDYVDAGKDVSLHGRIASALVTRPQAL
                     RRIVGNLVDNALKFGGAAEIRVAASQSGQVTVAVLDRGPGIPAESLEAVFEPFYRLEG
                     SRNRGTGGTGLGLAIARQLALAMDAVLSLHNRPDGGLEARLVLKGVSRIPVVRQAH"
     gene            complement(18050..18790)
                     /locus_tag="VHV99_01115"
     CDS             complement(18050..18790)
                     /locus_tag="VHV99_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3634090.1"
                     /translation="MENTDHVLIVDDDRGIRELLATYLEKNGLRVSLAANGRQMRTVL
                     EQGAPDLIVLDLMLPGEDGLVLCRELRAGKFRAVPVLMLTARSEEADRIVGLEMGADD
                     YLSKPFAVRELLARIRSVLRRARMLPPGMQVTEAAPMIGFGDWRLDTTARHLLDAEGT
                     MVALSGAEYRLLRVFLDHPQRVLTRDQLLNLTQGRQADPFDRSIDLLVSRLRQRLQDV
                     AREPRYIKTLRSEGYVFSAAVTMIEGDS"
     gene            complement(18916..19287)
                     /locus_tag="VHV99_01120"
     CDS             complement(18916..19287)
                     /locus_tag="VHV99_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX3634091.1"
                     /translation="MSTELLQGSCHCGTVKFEVRTNVLPASRCNCSLCRRKGALMTPP
                     FAAGELKILQGEEALTLYQFNTRTAKHYFCKHCGIYPFHQTRKDPQLWRANIGCLTGV
                     DPYTLEASVANGASLSVVEDA"
     gene            19515..20096
                     /locus_tag="VHV99_01125"
     CDS             19515..20096
                     /locus_tag="VHV99_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX3634092.1"
                     /translation="MLSRLKTTTAVALFAATVAAGAAAIATLVASPAASAASTPTAKR
                     VDNNTTAPEFTGIDKWLNSDPLTLQQLRGKVVLVDFWTYTCINCIHVLPYVKSWNQKY
                     KDQGLVVVGVHTPEYPFERDTDNVRKAIKRFDITFPVAQDNRYATWNAYGNQYWPAFY
                     LVDKKGHVVYTHFGEGDYEQTEAKIKALLTEKS"
     gene            20136..21497
                     /locus_tag="VHV99_01130"
     CDS             20136..21497
                     /locus_tag="VHV99_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018438348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX3634093.1"
                     /translation="MSDTILPQSPSRRRFVSVAAATVAAGSLSQPAFADPDQAMIQIT
                     ESDGGVKTAIRPLRVHVPESQLIDLRRRIKATRWPERETVADDSQGVPLAMIQELARH
                     WATDYDWRKCEAKLNSLPQFVTEIDGLDIHFIHVRSKHDNAMPLIVTHGWPGSVIEQF
                     KIIDPLTNPTAHGASASDAFHLVIPSLPGYGFSGKPTTTGWGPERTARAWVVLMKRLG
                     YARFAAQGGDLGGVVANVMGKEAPPELLGIHVNFPATVPAGIAKALQAGDPPPPNLSD
                     DEKHAYEQLSSAAMKRRAYALEMGTRPQTLYGLADSPVGLASWLLDHGDGYVQPAAAL
                     TSAVFGHPVNGESAGELTRNDVIDDITLYWLTNTGISAARFYWESHFNFLAAADVSVP
                     AAVSVFPRENYQAPRSWTEQAYHNLIYYNRLDRGGHFAAWEQPQLFAEEVRAGLRPLR
                     QRQ"
     gene            complement(21550..22314)
                     /locus_tag="VHV99_01135"
     CDS             complement(21550..22314)
                     /locus_tag="VHV99_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013338973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3634094.1"
                     /translation="MQRLNGKVAIVTGASAGIGRATAKLFAAEGAKVVIAARRAAELE
                     TLAAEIVREGGEAAFLAGDVQSEDYAKALVELAVSRFGRLDIAYNNAGTLGEMGPTTG
                     VSEAGWSAALATNLTSAFLGAKHQIPEMLKQGGGSIIFTSTFVGYSFAFPGTAAYAAS
                     KAGLIGLTQALAAEYGPQGVRVNAILPGAVDTPMYRDMNDTTESQAFVTGLHALKRVA
                     RPEELARSVLYLASDDSSFVTGTASLVDGGASITRT"
     gene            22454..23371
                     /locus_tag="VHV99_01140"
     CDS             22454..23371
                     /locus_tag="VHV99_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3634095.1"
                     /translation="MDRLRAFEVFVTVVSRGSFTRAADALDTSPANVTRYVNELEAHL
                     GTRLLNRTSRRLSLTEGGETFYARCKSILDDVAETEGLVSSASVEPRGRLRINAPVSF
                     GILHLAPLWPAFMRKYPEVELDVALIDRVVDIVDEGYDLAIRISRAGSTSHAARKLAT
                     SRNILCASPAYLARCGYPAAPADLIEHRCIGYSYAATGDEWQLADSEGKSHAVKVNCH
                     MHTNNGDTARAAALAGQGVIWQPTFLVGNDLRAGTLVQVLPAYRLPDIDVLALYPSRR
                     HLSAKIRAMVDFLAEAFGNVPPWDRADGD"
     gene            complement(23491..24426)
                     /locus_tag="VHV99_01145"
     CDS             complement(23491..24426)
                     /locus_tag="VHV99_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012218417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3634096.1"
                     /translation="MNEVRAITIFVRAAALGSLRKAAVDQGISPQAASHAVMQLEKEL
                     GVRLFHRTTRKLSLTEEGQGLLDSVKPALAILSSALDDARRSKDEIAGPLRVSAPRAV
                     GHTVLWPYFLEFAELHPNVQVDVQFDDHFTDLVSDRADVGFRGGSPPSGGTIARRLLP
                     IQLIVCASPGYIERHGAPRTIDELDGHRCTGYRRANTGKQAPWEFLIGDEIVYRDVAT
                     ALCVNDTDAETQAVLAGLGIGQLGSFSATLPIRRGQLVPLLVKHVTQREAIYIYYRHR
                     TEQPLRVRTFIDFMVGRLAGNRNFYLEPSELRAAR"
     gene            24543..25400
                     /locus_tag="VHV99_01150"
     CDS             24543..25400
                     /locus_tag="VHV99_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEX3634097.1"
                     /translation="MVGNTKNGFKRVWFITGASRGLGALIAQAALADGNAVVAAGRNV
                     AAITERFGNSPALLPVALDVTNEAQAKAAVQATVERFGRIDVLVNNAGFGLLGAIEES
                     SDADVRRMYDTNVFGLLNITRAVLPVMRAQRAGHVINMSSIGGYWSAAGFGAYCSTKF
                     AVEGLTEALRTELKPLGIHATVVEPGYFRTDFLDASSLVVAGNVIADYDETSGNVRRV
                     ATSLNHNQPGNPEKLAAAMIELVDAQAPPLRLPLGTDTLKAIAEKNAYVTQETETWRA
                     LSASTDFSA"
     gene            complement(25433..25831)
                     /locus_tag="VHV99_01155"
     CDS             complement(25433..25831)
                     /locus_tag="VHV99_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634098.1"
                     /translation="MTTSQFDACNNAHDAEIEAQLCAYNCAFDELGLRFRWDARTLAQ
                     LAAIDGEEARIAAYIEAHHAHLLKAYSVGFLSRVILERKNARYPACLPTRVDNAPQPS
                     HADHLAHQSRMSSRRERVSYEAELPALAGV"
     gene            26407..27738
                     /gene="oxlT"
                     /locus_tag="VHV99_01160"
     CDS             26407..27738
                     /gene="oxlT"
                     /locus_tag="VHV99_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013094653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate/formate MFS antiporter"
                     /protein_id="HEX3634099.1"
                     /translation="MGHSNVPGRSSATFRQNRWVQLAIGIVCMGLVANLQYAWTLFVV
                     PMDNAHHCGQAAIQTAFTVFIVTETWLVPIEGWLVDKFGPRPVVIGGSLCAALGWIID
                     AHASSLVELYIAAVIAGIGAGCVYGTCVGTALKWFPDKRGLAAGLTAAGFGAGAAVTV
                     IPIANMIQRSGYEHTFMFFGIFQGVCILLLATMLVRPKPPATAVAAKRVVSSKIDYTP
                     GEMIRSPLFWVLYLMFVFVAAGGIIATAQLGPIAKEYGFAKMPVNLLGITLPLLTMTL
                     SIDNLCNGFTRPLCGFLSDRIGRENTMFMIFLGEGVALLGLMQFGHNPYAFMFFAAAV
                     FLCWGEIFSIFPATCADTFGSKYAAANAGTLYTAKGTASMLVPIASVLSANGGWSTVF
                     ISAAVVSIAAAVSAKFILAPMRKRWIENSGAPAGAVLAEARLQPSSSTPSE"
     gene            27803..29545
                     /gene="oxc"
                     /locus_tag="VHV99_01165"
     CDS             27803..29545
                     /gene="oxc"
                     /locus_tag="VHV99_01165"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HEX3634100.1"
                     /translation="MAEADSPTTENKLKQQATETTDGFHLVIDALKLNDIDTIFGLVG
                     IPITDLARLAQAEGMRFIGFRHEQHAGNAAAIAGFMTQKPGICLTVSAPGFLNGLTAL
                     ANATTNCFPMILISGSSEREIVDLQQGDYEEMDQLNAAKPYAKAAYRVLHAEDIGIGV
                     ARAIRAAVSGRPGGVYLDLPAKLLAQTLDAVKAQQSLIKVVDAAPRQLPAPESVKRAI
                     DVLKSAKRPLILLGKGAAYAQADAEIRAFVEQSGIPYLPMSMAKGLLPDTHAQSASAA
                     RSFVLQEADVVVLIGARLNWLLSHGKGKTWGAAPKRFVQVDISPTEIDSNVAIAAPVI
                     GDIGSCVAALRAGLDAGFAKPSTEWTGAIAERKSKNLEKMAATLDKNPSPMNFHSALR
                     AIRDVLKTRPDINVVNEGANTLDYARSIIDMYEPRKRFDSGTWGIMGIGMGFAIGAAV
                     TSGKPVVAIEGDSAFGFSGMELETICRYELPVCTIVFNNNGVYRGTDVNPTGGKDVAP
                     TVFVKGARYDKMIEAFGGVGHHATTPEELTKALLEAIASGKPTLINAVIYEAAGTESG
                     RLTNLNPQSAAMKK"
     gene            29571..>29842
                     /locus_tag="VHV99_01170"
     CDS             29571..>29842
                     /locus_tag="VHV99_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX3634101.1"
                     /translation="MTKPLEGIKIIDFTHVQAGPACTQLLAWFGADVIKVERPGSGDV
                     TRNQLRDIPDADALYFTMLNSNKKSLTLDTKKPEGKEVLEKLIRES"
BASE COUNT         5584 a         9592 c         9069 g         5597 t
ORIGIN      
        1 atgggaacgg ccaatgcaag tggaaggtaa gaccgccacc tcggtcatct atacctacaa
       61 gatcgatccg tcaccgtggg ccaaagatgc cgatgcgcag cgcgtgttcc cgatgatcaa
      121 gcgcgtaatc gagggcgcgg gcacgttgca gttgcgcgaa ggcgtgcatc tgacgtcgaa
      181 cggctgggtc gccgacgaag tcttcgtgcg ctgaaacgca ctgcgcgggg cgcgcgttta
      241 gccgccccgc aggcctttat gccaggttac gccgggttac gcctgatcac gcggcacgaa
      301 acggcgagtg cgaatccatt tggtggccac ccacttctcg ccagtccgca agggcgtgct
      361 tgcatgcagg gagctcggat cggagagccc gaaacggttg ccatattcga aatagagcgc
      421 ctggccacgc tgcggcacaa ccgaccatcc aagctgcggg aacacggttt cgccgccgcc
      481 ttccacatcg ttcagataca tcagcagcgt gcccacgcgc tgcccgctac gcgtgatcga
      541 ctcctgattc gcggggttgc ccgggatcag ataatcgacg tgcggagtcg attcggcgcc
      601 cgcttcgtaa tgcaggagtt gcaggccttc gccgttctcg gcgggcaggc ccgtgagctc
      661 ggcgatccgc gcttcgaggc gcgcgatcag tggcgtctcg gccagacgaa aaaacatccc
      721 ttcgctgctg cggtggccgg ccacgacatt acggccggtg acgggatcga ccacggtcga
      781 atggttcaga cgcggccgcg cgagcgcgat cagttgctcg cattcgttcg cactcagaaa
      841 gtcgtcgagc agtaccgccg cgggccgctg catccgcgaa atcacgcgcg tcttgcgctc
      901 gccgagccgg atcagcggtc cgtccggcac gcgcagcggc tccggttcgt aagtcaccgg
      961 cgcgccgcca atgtcgagcg tctcgcccgg caacggtatg ccgtataaaa aagagttgga
     1021 gaccgcatcg accatcgccg ttgcaagttc gaccgcattc ttctgattga ccaactcgcg
     1081 cacaatggcc tgcggaggaa cgccgcgcgt gagattgtga gcgatccaat cgcgcacttc
     1141 ggcgggaaag ttaacggtta tggacatcgg cgtgtcgagt taaagctgga agggttcgat
     1201 tatctaatgg aatcgatgac aatccggttg acggctttga tcgggccgcg cgccggacac
     1261 taacgcactc gatcaaagag tggagaggcc aggctggcgg ccgcgagttc gttggcggcc
     1321 gccacaagcg cggggccgag cgccgtcatg cgcttctccg tcaatctaac cgcgggccct
     1381 gcaatactga tgaccccgat agccgggtaa cctcttcgtt ggaccggagc ggccatcgcc
     1441 gtcatccccg gcgcgaacac ttcataaatc gttgcgtagc cacgggcgcg agcggcatcg
     1501 acgaacccca gcagcgcctt cacggtagtc ggtgcgttgg gtccgtactg cttcggcttg
     1561 ccgaacccct ggcgcgtcac caattcgagc gcccgctcat cggtcatggt catcatccag
     1621 gcgtgaccgg ttgcactgca agacagaatc gtgtccatac ccatgtccgg gtcgtaacgc
     1681 aggcccttca gcgcgccctg cgacttggca acccacgtca gcctgtctcc gtcgacgatc
     1741 gctaatcgca cgagttctcc ggatgtttcc gccagacggt cgagcatggt ttgcgcaatg
     1801 tcgatgatgc cggacgtcgc gagataactg agaccgagcc ccgcgagttt cgtggtgagc
     1861 acgtagtcgc cgtgctcacg tagttgacgg atatagccgc attgcttgag atcgacgagc
     1921 aggcgatggc aggcactgcg agggatctcg agctgatccg agatcaacgc gagtggcgtt
     1981 ccttccatct gtgtcgccag aaattcgagc accgccatgg ttctttcggt cacgcccggc
     2041 atggagactc ctctcaactc agcagagcga gcatgattgc ataatgcgaa accaaattcc
     2101 agcatggaat aacgtcccac cagcgctgtt aatctgtgcc gcatgccgtc tcatcgagtg
     2161 cttcaccacg cacggcagga gacgcataac aacagacggc ccgctcgcgg tcgacatgac
     2221 aagggccacc ggaacgcgac gccgacacag gtttggaaca ggagacaaca tggatcagca
     2281 attacaacaa cccgcgcatc aggcgatgcg cacgcgtgca gtgaccgccg cggccatcgg
     2341 gaccgcactc gaatggtttg atttcacgtt atatggcgcg ctagccgcca ccgtactgcc
     2401 caagctgttt ttcccatcga tggattcgac ctccgcgttg ctggcatcgc ttgccacatt
     2461 cggtgtcggc cttgcggcga gaccgctggg cgccatcatt tgcggctacc tcggcgacaa
     2521 gttgggacgg cgcaatctga tgctcggcac cgtcaccgtg atgggtgtct gctcgatgct
     2581 gatgggcctt ctgccaacct atgcgagcat cggcgtatgg gcgccgctcc tgctggttgt
     2641 gcttcgcatt ctgcagggtt tcgcgctggg tggtgagtcg acgggggcgc agttgatggc
     2701 gatcgagcac gcaagtcccg atcgtcgcgg caaatattcc ggccttctcg gcctgtgttc
     2761 tccgctcagt cagatcatgg ccaacggcgt attgctggca ctgtcatcgg cgatgtcggc
     2821 gcaggcattc gatgagtatg gctggcgcat tccgtttgtc ctgagcttta ttctcgtgat
     2881 tgtcggcatc tacatccggt tgcgagtcag tgagacaccc gcattcgtcg cgctccgtaa
     2941 aaccgaagtc gctcatgtgg gcagtccgct gcgcgatgca ctgcgtcttc actggcgaac
     3001 cgtgctgcgg tggatgctgt tcttctgcgg ccctgcggca atcttctatc tgatcgtcgt
     3061 gttctcgttg agttatgtca cgaaaacgct gggcgtgccg aaacagacgg gcttcctgct
     3121 gttgatgggc gcgaacgtgt gcgcgatcgt cggcgcgcta gccggcggca tgctgagcga
     3181 ccgaatcggc cgcaagaaag cactcgcgat cggctcgatt gccaccttgc tcatgctgtt
     3241 cgtgtacttt cagattctcg atacgaagag cttcgtgccc atgctggcag caatgggctt
     3301 ctttctcggc ttcacccaat tccagagcgg cattcagccg gttgcattcg ctgaagcatt
     3361 tccgaccaac gtgcgctact caggctcggc gcttgcctat accggcgcga atctgatcgc
     3421 cggggggccg atgccggttc ttgcggtctg gctcttcgcc gtttgcaacg gctcgccgtg
     3481 gggcgtcgtc gcggtgtgcg tcacgttcaa cgtcatttct ctcatcacga tcctcaccgc
     3541 tcccgaaacg ctgggtatcg acatgaaccg ggtcgactct acggaacaag tcatgggctc
     3601 atcagagcac gtgccgaccc ggtcccattc tcactaaaaa tgcaggcaat gcagatgaaa
     3661 ccacttgttt atgtgctgaa cggttcgaac ctgaacatgc tcggcaagcg cgaaccgcac
     3721 ctgtacggta cgacgaccct cgctcaggtg aaagagcgca ctgaagcgct ggcggccgaa
     3781 ctcgacctcg aatgccattt tcgccaaacc aaccacgaag gagtgatggt cgactggttg
     3841 caggaagcct tcgagaagga tgcggccgtc gtcatcaatc cagcagggtt ttcgtttgcg
     3901 tcgatacccg tgctggatgc ggtcaagctg attcgccaac cggtggtcga ggtgcacatc
     3961 accaacatcc atcaacgggg cgagcagtat cgccattcgt taatctcgtt ggccgcaggc
     4021 ggtgtgatat gcggccttgg ggttgatgga tatctgctcg cggtgcgcgc ggtcgcagga
     4081 gccatccagc gcgcaaaagt gtgatggcgc ggagcgctaa ttgagggcaa agcgctccgc
     4141 tcgttgcgcc gacgtctgtg ctcaagcaat ttccgcagcg agcagatctt cgatcatgat
     4201 cggcaacttc cgcacgcgca cgccggttgc atgatgggtc gcttcggtaa tcgcggccgc
     4261 tatgcccgca aggccgatct ccgcgatacc ccgggcgccg agttcattga acacggtgtc
     4321 cggatgatcg agaaacgtca cgtcgagttc gggcgtgtcc gcgttcgacg cgatgatgta
     4381 atcggccagg ttgctattga tcggtgcgcc cgtctgccgg tcgtagatcg tgtgctcgaa
     4441 cagtgccatg cccacgccca tcacaacagc gccctcaatc tggttgcggg ccgcgcacgg
     4501 gttgatgatg cgtcctccat cgatcaccgt gaccacgcgg ctgacgcgca actgtgcgat
     4561 ctccggctgc caggtgactt cggcgaagtg cgcgccgtaa gaatagatgg agaagcgctt
     4621 tttgagcgga tcgtcgaaac cgcccttggc actcccactg cctgacgccg cgtgcatctt
     4681 cgctgcttcg agtatctgac cgaacggtac gccgcgctcg ggtgcctcac ccttgcgatg
     4741 cacgtgagcc gcgctgaacg cgagcccctc ttcctcggcg cctttgaacg gcgaaccttc
     4801 tacggccgcc gcgcgcgaca acaccagctt gattgccgca cgcaccgcgt cgaggaccgc
     4861 gggaatcacc gatgccgtcg ccgcgctgcc gcccgaaatg ggccccggcg gcaacgccga
     4921 atcgccgaga ccgacctcga tgcgctcaag tggaatcccg gtctcttcag cgaccagttc
     4981 cgcgagcacc gtgtacgtgc cggtgccgat gtcctgcgta ccgcatacca cccgggcgct
     5041 gccgtccacc cgaagatcga cgtccgcggc agcggaaaag cgcatccccg gccagacaca
     5101 ggcaccgacg ccccagccca gggtcagccc gtcacgatgc atcgatccga cgcctggcgt
     5161 ccgctgtgac cagccgaacc gttcagcacc cgtgcgcaaa cactcgacga gatgccgtga
     5221 agagaacggc aaacccgtgc tttcgtcgac gcgcggttcg ttgcgcagcc tcaattcgac
     5281 gggatccatg tgaagcacct gcgccagttc attcatcgcc gattcgatcg cgtacagccc
     5341 gggcatggcg ccggggccgc gcatcgacgt gggagagccg acgttgcggc gcgcgagcgc
     5401 ggacgtcacg cgcaaattcg gcgtgctata gatgagcggc gtgacttcgc cgcagccttc
     5461 ctggtactcg tcggcgatgg cggtgtggtt gatgtagtcg tgctgcaggg aggtgaggct
     5521 gccgtcgcgt tccgcagaca gccgtacgcg ctgttgcgtc acgggacggt ggccgacatt
     5581 ctggaacatc atcttgcggc tgactaccag cttcaccggg cggccggtct ggcgcgccgc
     5641 cgcagccgcg agcaggctgt gcggccacat ccatagcttt cctccaaaac ccgatcccag
     5701 gtagcgggag atcacgcgga tcttttcctt gggcacgccg agcatctgtg acagcgtacc
     5761 caggtgggcc gaaatggact gcgtcgtttc gtaaaacgtg tagctttcgc cgtcccactg
     5821 cgcgatcgtg gcgtgcagtt cgatggggtt gtgggtctcc gtgggcgtca cgtaggttgc
     5881 gtcgagcttt gccgacgcgg cgtcgaacgc gccgtccggg tcgccgcgct cgctctcgac
     5941 cttgggcgtg tcgtcaggct cgagttcgcc gcgcacatcg tgtggggtga tttcgtagcc
     6001 ggccttgatc gccgcggatg ccgcgcttgc cgcctcgaag gtgtcggcga tcacgaccgc
     6061 gatgtactgg ccgtagtagc gaatgatgtc gtcctcgaag ggcggccggg cttcgtcgac
     6121 atagccggtc tccaacgagt tgccggagat ccggtagaag cggccgatat tgcctcgatg
     6181 cagcaccgcg tggatgccgg gcatcgcctc ggcatccgcg atgtcgagcg aggtgagccg
     6241 gccgctcgcg atcgtgctgc acaccggcac ggcaatcagc aggccgggca ggtccacatc
     6301 ggaggtatag gttgcgctgc cgctgacctt cagcggtcca tcgacacgcg catgaggccg
     6361 tccgatcacg gacagcacgg agtcaggcaa ggtaggcatg gtgcgttcct ctcgatcgtc
     6421 ggttcaggtc gtggccgtcg cctgggtcag tgcgtggatg atgcagcggc gcgccagctc
     6481 gaccttgaaa ccgttctggc tttgcggctt tgcgttcgcg agcgcggcgt ccgcggcttt
     6541 gcggaagatc gcggcgccgg gcgtcgcgcc ttgcagcagc gcctgcgctt cgagcgcatg
     6601 ccatggcctg gtgccgacgc cgccgagcgc aacgcgcgca tggccgatgc gtccaccgtc
     6661 gagcgtcacg accacggcgg ccgacgccag cgcgaactcg tacgacgcgc gatcgcgcag
     6721 cttcagatag accgaccgcg tgcccgccgg cagagggggc agggtcacgt gcgtgatcag
     6781 atcgcctgcg ccgagcacgt tctcgcgctg cggcgtcgcg cctggtaaca ggaagaagtc
     6841 gtcaatcgcg atgctgcgcg cgccgtccgt cccttcgacg tgaatggtcg cgtcgagcgc
     6901 ggtcagggcg acgttcatgt ccgacgggtt ggtggcgatg caggcgtcgc tggtgcccag
     6961 aatcgcgagt gtgcgattga acccgtcgat agcgggacag cccgaacccg gctcgcgctt
     7021 gttgcacggc atcgcggtgt cgcgaaagta cacgcagcgc gtgcgttgca gcaggttgcc
     7081 gccggtcgtc gccatgtttc gcaattgggc cgaggctccc gagagcaacg cctgcgacag
     7141 taccgcgtac ggctcgcgga tcagcggatg caacgccagg tccgcatttc tcgcggtcgc
     7201 accgattttg acgccgccgc cgggcagcgc ttcgatacgg tcgaggggca gccggctgat
     7261 gtcgatcacg cgcaccggcc gctcgacatt cagcttcatg agatcgagca atgtcgtgcc
     7321 gcctgcgaga aagcgtactt gtgcgccttg ctgggccgtt accccggccg cgcccgccgc
     7381 gatcgcgtca ggcacgccgt cggcgcgtga caactggaac atttccatga tggcgctcct
     7441 caggactggc cgcgcacgcg ctggatcgcc gcgacaatgt tctgataggc gccgcagcgg
     7501 cacagattgc cactcattgc ttcgcgcact tcagcattac cgggcccgac cgcttcatcg
     7561 agcagcgcga ccgcggacat gatctggccc gacgtgcaat agccgcactg atagccgtcg
     7621 cattccacaa acgcagcctg catcggatgc agcgcatcgg gctgaccgat cccttcgatg
     7681 gtggtgattt cgtcgccgcc atgatgcccg gcgaacgaca ggcacgcatt cacgcgccgt
     7741 ccgttcacat gcaccgtgca cgcgccgcac tgaccgtgat cgcaaccttt tttggtgccg
     7801 gtgaggtgca gatgctcgcg cagcgcgtcc agcagcgtgg tgcgcggatc gagcgacagc
     7861 tcatattcgt gcccgttgac tgtcagctcg actgacatcg tcgtgttggc ggaaggctgg
     7921 agcacggtac gatttgcgtc gcgctcaacg ctttgttgcg gggtgtgaat gggggaggcg
     7981 gtctgttgca tcatgcattc tcctcatggc acgagcggcc agaattggcg cgcccggtgg
     8041 cggacggaag cagaaagcgt ggcccgttgg cgatcggtcg ccgggtcgtg ggccgttcgg
     8101 ggggcgagct tcgggttgca tcacgcccgc gcaagcggga gagcagcaag cgtaatgccc
     8161 gcccaaatga cgtctgaaca tcagagagtt tagtgtgcgc cgcggcctgc gggtaacact
     8221 ttggtcagcg gcaccggctg cgtttgcgtc gacgggacgg cacccgttgc aaatgggtcg
     8281 actgagagat cgctgaaatt ctcagccacg gacaccgaat cccggccgtc cgtgattcca
     8341 tggctgcgtc gccaactggc gggcggcatg ccaaattccc gcttgaaagc gcggttgaac
     8401 gctgcctccg agtcataccc cacccgctcc gcgacggccg cgagtgagcg cgtcccgttg
     8461 cgcaactcct gcgccgcgat ctgcagacgc cagcgcgcca gatactgcat cggcggcaga
     8521 ccaagcagtt gcgtgaagcg ctgcgccagt gccgaacgcg agaggcccgc cccgcgcgca
     8581 agttcgtcga cagtccagcc gtgcgcgggc tgcgcgtgca gcagcgcgag cgcgcggcca
     8641 acgtaacgat cgccgacgcc cgccagccaa cccttgcgat cgtcgggcag cgcgtcgatg
     8701 caacgccgca cggcctccac aaacagcaac tccgacagac gcgcgagtac gatcgcgctg
     8761 cccacgcgcc cctcgcccgc ttcaactgcc gcaagcttta acgatgactc gagccacgcc
     8821 gattgcggat cgttgcgcat gtccaccttg aagatgcgcg gcagcgccga aagcaccgga
     8881 ttcgtcaacg tatcgtcgca ggcaagaaag ccgcaaatga gacgcgtgcg tgccccgcca
     8941 ccgccgtagc tcaactccat cacttcgccc ggcacggttt tcaggaactg cgacagcatc
     9001 tcgtccgagg gtgcgggcga gagttcgagc gagctgccca acagatgcgc ttcgccttgt
     9061 ggcaccacaa gaagttcgcc cgcatcgacg cggatcatgc aagtgggatc gccaacgagg
     9121 cgggcgcagc agctcccttc tgtaatgagg tgataagaga cgatgcgttc ggcgcgcggc
     9181 agaaacgtgg cgtacaacgc cggcgtcgct tcgccggcaa cacaccaggg tgcagtgaat
     9241 tcgccattta gataaaccgc tccgcccaga cgaacgaggc gaaggacgtc cgaaagagca
     9301 tccatgaaag cgcccatcaa tttcggcgtc ctgcgcaaaa catctggacg cctggtcata
     9361 tatcaatgca acttgcgtcc cgatactagc actcacgcga acccacgaca acgaaccgtg
     9421 gaatgttcca tcaacacatc atcaggatac cgacatgagt cacgcaaata cctatcgtgg
     9481 tcaatgcttt tgcggtgccg tgcaatttac ggtgagcgga gaaccggcgg gaatgggcta
     9541 ttgccattgc gattcatgcc gacgctggtc gggcggcccc gtcaacgctt tcacgctctg
     9601 gaagcctgaa tcgttgagcg tgacgcgcgg cgccgatgat atcggcactt acaacaagac
     9661 gccgcacagc taccgcaaat ggtgcaggtc gtgcggtgga catctcttta cggagcaccc
     9721 gggactcggc ctcatcgatg tgtatgccgc cgtcatgacg gatttcccgt atgaagccgg
     9781 tgtgcatgtt cactacccgg agtcggttct tcccatgaac gacggcctgc cgaaattaag
     9841 agatctgccg cgcgaaatgg gcggctcagg cattagcgtc ggtgagtagc cgttgcattt
     9901 cacggaacag tttctgattt gtccgatcgt cgcgcggacc tcctcgcagt gcaaattcgc
     9961 ggcgccatca ggcagcaaaa agccatgcat tcccgcatcg ctgccgtttt tgcactcagg
    10021 agttttttat gtccgctgtc gattcctctt tctcgcccgt cgcagacttc actgccgtca
    10081 aggtccgtca acaggccgcc tggtcaaccg gcaactatgc cgtcgtcgga accacgctgc
    10141 agatcgtcgg agaaaatctt tgcgaagcgc tcgacttgcg cgccggaagc cgcgtgctcg
    10201 acgttgccgc gggcaacggc aacgcgacgc ttgccgccgc gcgccgctgc tgcgacgtca
    10261 tctccaccga ctatgtcgcc tcactgctgg agtcggggcg cgcgcgtgcg caagcagaag
    10321 ggctcccggt gcaattccag gaagcggacg ccgaagcgct gccgtacgcg gatgcctcgt
    10381 tcgacgtcgt gatgtcgacc ttcggcgtca tgttcacgcc agatcaggag aaggcggcag
    10441 ccgaactcgc tcgtgtgtgc aagccgggcg gccggatcgg tctcgccaac tggacaccgg
    10501 agagcttcat cggccaggta ttcaagacga tcggcaaaca catcacgccg cccgcgggcg
    10561 tggtgtcgcc agcgctctgg ggcacgaagg aacggctgca cgcgctgttc ggtgcgaacg
    10621 cccggcagat cgtcgcgact agccgctttt tcacgtttcg ctaccgttcg ccggcgcact
    10681 tcgtggacgt gttccgcacg tactacgggc cgatgaacaa ggcgtttgcc gcactcgagg
    10741 gtgaccggca ggcagcgctg cttggcgatc tgatgacatt gatcgagagc cgcaaccggt
    10801 cgggtgactc aacagtcgtc cttccaagcg agtacctcga agtcgtggtg gagcgatcat
    10861 gaacaaagtc aggcgtgaag tcgccgcatt ggtcagcgcc ggctcgcgcc tctggtgttt
    10921 ggcctgcctg attggccttc ggcatggctc gatgctggtt gccgtgccgc tgtgctcgtc
    10981 ggtgtgtttc ttcagtgtcc ggttcgcttt gtcggcgctt ggcctctctg tttagatgcg
    11041 gttttcccga gcttgatggc ttcgctgcgt ccgcgcgcgc ggcgaagcag tctgcccctg
    11101 ggattgacag aacgcagacg tccacgcctc gccgttgtca ctgttattga cggcaccgcg
    11161 tgagaacacg tgtttatccc gacccaacta tcgctctaaa tctattggcc gcgctacgac
    11221 gctttgcccg attcgcatct tctcaaggcc gctcaatgcg cgccagacgg agcttgctgc
    11281 aatgtggcac ggactctgca atacacaacg tccagccgta gcacgacgcg cccatccacc
    11341 gaacgcggat gcacccgtac ccgaatgcac gcctggaacc cgcgtagcgc gacctgttcg
    11401 cgccgatacg caatcgacca ggtcaccgac tggaggacat catgttcatc tatctttttc
    11461 tgctgctgag aaaggcgctc gaacgcgctg aacacagccg tcgagacgcg tacctcgcgt
    11521 ccgccgtcga catcggcgag ctagaacgcc gtatgcattc gatcgacttt gacggctgag
    11581 atgacaaacc aacgcgccct gcaccgatca gtccggagac cggatcatcc gcgccggcgt
    11641 cccgattaga accagagctg cccacgccgc accgatagta gagactccgc cggtccggca
    11701 tccgtgcgcc caacccgaga gttccatgaa cgacatgaac gacatgaata gcactatcgc
    11761 atcgcaagtc atctctcccg caacaacggt gatcgcactg cattgctccg gctccggagc
    11821 ggcgcaatgg cgcaagctcg gcgaggccct cggctcgcgc cacgcgttag tcgcgcccga
    11881 gcactacggc tgcgatagca cgggcccgtg gagcggcgag cgcgcgttca cgctcgccga
    11941 cgaggctgcg aggaccatcg gcatcatcga cgccgcgcgc ggcaaggtcc atctcgtcgg
    12001 ccactcatat ggcggcggcg tggcgttgcg cgcggccgtc gagcggccgg ggcgcatcgc
    12061 gagcctcacg ctttacgagc cctcggcgtt tcatctgctc aaggtgatgg gcgcgcacgg
    12121 cgcgtacgcg cttgcggaaa tcatcgcgat ctcgaagcgc accgccgacg gcgtgagctg
    12181 tggcgactat cgaggcgcgg cggcttcgtt cgttgattac tggggcggcc ggggttcatg
    12241 ggaagcgctg cggccctccg tgcaggccgc gctcacgcgc tgggcgccga aggcgccgct
    12301 cgacttccgc gcgttgctcg acgagcgcac gcccgcaagc gcgtacagga gtctgcgtgt
    12361 gccgacgctg atcatgcgcg gccagcacgc gcccattccg acgcgcgcga ttgctgagcg
    12421 actgccgatg ctgctgcccg cggcgcgtct tgcaatcgtc gagggcgcgg gacacatggg
    12481 gccgctcacg catgcagaca aagtcaacgc gaccatcgta gggcatatca ccgagtcgga
    12541 agcggcggct tgaacggttc gcctcccctg cgccgaacag cgcagcccac ctttcgaacc
    12601 aggacccagg cgcctcaccg ctcaaccttc gacataaccg ccgtcgccgg gtcataccga
    12661 taacccttct gcgaaagtcg ctcggccatc gtcgccccga tcagcttttg ctgcgtgtcc
    12721 cgggatatca gctcactatc gggcttaagc cgctcaagct cagacgaaag cttccggatc
    12781 agcgcgactt cgcctgtgct gcgcgagtcg atgaacagta gtttttcctg ggccgaaccg
    12841 agtcgggctt tgaggtcctt ggcatgcgcc acccactgtt cagcaatcgt gcgaagctca
    12901 ttaaaagctt tctcatgcgc tttggcctgt gccgagatct ggcgctgcaa ctcggcgatt
    12961 tcttcagccg ttgccgactc ctcgctgcgc gccgcctgac gtgctgccgc gagcgcggcg
    13021 cgcgcctcgt cccgttctgc ctccagcgcg gcgagccgcg cgacgctttc gtgcgcctct
    13081 gccgcagcag cctctgcccg cgacgaagcc gcgctgactt cctgcgtcca tcgttccgct
    13141 tcggccgagt tcgcctgctc gacttccgcg aggcgctgct gcaacgtcgc cgcgtgctgc
    13201 tcagcggcct gggcccgttc cttcgcggcc gacgcgtcct gcgaagcctt gaagagttca
    13261 gcgctcacgg cagccacatc ttgtgagacc ttgtcgagtt cagcgatcgc ggccgctttc
    13321 gcctcgctca tggcactcac ctgttctcgc gcctcttcgc gctcccgagc aacacgttgc
    13381 agtttgttca gctgaaccga cgcctcctcg ccccgcgccg tgcttgtccg gcgttccgct
    13441 tcgagcgcag tccgcgtctc cttcagttcg gcttcgagtc cagtgcgcgt ctcaatgagt
    13501 tcggcttcga gtcgaatgcg cgtctcattc agttccgcct ccagcgcggc aatccgtgca
    13561 aggctctcgt tcgcctgcgc cgcggcagct tccgcgcgcg atgaaaccac gctggattcc
    13621 tgcgcccatc gttccgcttc ggccgatttc gcctgctcga tttctgcgat gcgctgctgc
    13681 aaagccgccg aatgctgctc ggcggtctct gcccgctcct ttgccgcgga cgcctcttgt
    13741 gaaacccgag ccagttcagc gctcgcagcc gaagcgtcct gcgaaacctg agccaattcg
    13801 gcgctcacag ccgctttcgc ctccgtcatc gcgctgacct gctcccgcgc cgcctcgagc
    13861 tcccgggtaa cgcgctgcaa ttcctccaac tgaagcgacg cttcttcgct ccgcgccgcg
    13921 cttgcccggc gctcggcttc gagcgaaaca tgcgcctcgc ccaattcacc ctccagcgcg
    13981 gccacccgcg cgcgactctc atttgactgc gccgtagctg cttccgcgcg cgccgaagcc
    14041 tcgccggcct cctccaacgc ccggtccgtt tcagccgact tcgcctgaca gtcgtaaccc
    14101 aacgaggtga tctcctgctg cgccgcgtca agcgactgcg tcatccgcgc gatctcatct
    14161 ttctggttcg aaacagcggc ggccagcgct tcacgcgcct tgcgttcctc gtcgagttcc
    14221 gccttcgtgt gttccagcgc ggcctcttcc accgacacac gctgcgccag ttcctccgcg
    14281 cgcgcctcgg cggcgtcggc tcggccagtc gcggctgaaa gttccgcgct gaggcgcgta
    14341 gccgcctcct ccgaggcact cagcgaactc gtcagaccaa tctgccgctc acggctcgat
    14401 tcgaactctt ccagcatctt ttgatactcg gcaagcgcct cgtcccgttc cgcgcgggca
    14461 gtgtcgatgt gctgattcag cgcgctcagg tgttgactga ccgccttgtc ggcttcgccc
    14521 agggcggcca tccagagccg atccgtggcg ctcatcatgg tttcggcgac gtgctccggc
    14581 agcgcggttt ggatccgcgc atgaggaacg tcaggctgcc gcgcttcgcg ccagcgttgc
    14641 agagacgcgg caatggcaac catcgacccg ccgggaacct ccggccagat cgtcaccggg
    14701 gagaccttcc ggccctcttc gaccatgcga tccgcaatcc ccgcaatcag ttcatccgtg
    14761 attgtctctg catccgtgga cataagcgcc tcaaagctca atagacgtgc tagcaaatag
    14821 agtacccaat acgttactcc attcagccat attcccgatt tcccgatgaa tggagcgccg
    14881 gtttgctcag gccacgccgg cgagcaggcc gcggctttga cacggtctgc caaggccaac
    14941 gaaacccgat aagcttagct tcgtcctatc gcccaaggat ttcccgcccc gatgccctct
    15001 gccatccgca actcgctcat ctggatcttc gacgcattcg agcgcgattc aacctacgtt
    15061 cacaaacgga tgttcggcag cgatgccgcg tatatcgatg gcctgttgtg tctgatcgcg
    15121 gccgatcgcg agaagccgtg gaatggcttg ctggtgtgca catcgcaaga tcgccacgcc
    15181 gcgctgattg aagaagtgcc cgctttgcgg ccccatcctg tgctcggaaa atggctgtat
    15241 gtacagcagg acgatccggc gtttgaacca agtgtggaaa ggatgacggc gctcgtgctc
    15301 gcgcgcgatc cgcgcgtggg ggtcgagccg aaagcacgca aacgaggcgg gaaatccgcg
    15361 ctgcctagag aggcataagg gaggcataac ggaggcataa ggcttcgcag ccggcctctt
    15421 agctcttcgc ggcgcggctg gccgcggcct caaagcggtc aaatgaaagt cgacgaagca
    15481 gacgaccacg ggcaatggcg cgtcatgaag cgtgaaaatg cgccgtcctc attcattgca
    15541 tttcgtcgcc cgtctccacg ccgcttaaag ccgaacaacg ccgatgatgg cgtcggcaaa
    15601 tgccttcggc gcttcctggg gcaggttatg tccgatgccg ccgtcgatat tccggtgagc
    15661 atacttgccg gtgaatttct tcgcgtaggc tgccggctcc ggacgcggtg cgccgttggc
    15721 atcgccttcc atggtgatgg tcggaacgga aatggtcggc gctttcgcga ggcgcgcttc
    15781 aagctcgtcg tattgcggct cgcccttgac caaccccaga cgccagcggt agttgtgaat
    15841 caccaccgcg acgtgatccg gattgttgaa cgactcggcc gagcgatcga acgtcgcgtc
    15901 gtcgaaattc catcttggcg aggcgagacg ccagatcagc ttgttgaagt cgtggcggtt
    15961 cgcttcataa cccgcctggc cgcgctccgt ggcgaaatag aactgatacc accaggccag
    16021 ctcagccttc ggcggcagcg gggctcggtt cgcttcctga ctgccgatca gatagccgct
    16081 caccgacacc atcgccttgc accgctgcgg ccacaacgcg gcaatgatat tgaccgtgcg
    16141 cgcgccccaa tcgaagccgc caaggatcgc ctggtcgatc ttcaatgcat ccatcaacgc
    16201 gatgatgtcg acggccacca ccgcctgctg accgttgcga ggcgtatccg cggacaggaa
    16261 ccgcgtcgaa ccatagccgc gcagatacgg cacgatgacc cgatagcccg cggcggccag
    16321 caatggcgtg acttcagcaa agctgtagat gtcgtagggc cagccgtgca gcagaatcac
    16381 gaccggtccg ttgcgcgggc ctgcttccgc ataaccgata ctgagtgctc cggcgttgac
    16441 ctggcgaata gcgccaaatg aagcaacctg tcccgtcgct ttcgtattgg atgtcgtgct
    16501 gttcgactgc gcctgggcga gtccgctcag gcccaactcc atcaaactga tacccgcaac
    16561 agtcgtccca agcagacgac gacgtcgaat attgatctgg tccggcatcg ctgattctcc
    16621 tcaaaaatta gccgctcacg cgcagagcgc gattcgcaca tgcgttcgat gaatgttgaa
    16681 aagccaccgc atacagcgca atccagtcag tgagcctgtc ggactacagg aatccggctg
    16741 acgcctttca acacgagcct ggcttcgagg ccgccatcgg gacggttatg caaggacagc
    16801 acggcgtcca tcgccaaggc gagttgccgt gcaatcgcga ggcccaatcc ggtcccgccg
    16861 gttccgcggt tacgcgatcc ttccagccga tagaacggtt caaacactgc ctccagtgat
    16921 tcagccggaa tacccggccc gcgatcgagc actgcgacgg tcacctgtcc actctgcgaa
    16981 gcggcaaccc ggatctcggc cgctccaccg aacttcaacg cgttgtcgac gaggttgccc
    17041 acgatccggc gcagcgcctg aggacgcgtg accagcgcgc tagcgattcg cccgtgcagc
    17101 gagacgtcct tgccggcatc gacgtagtcg aacacgagac tgtcgaacag cgcatctgga
    17161 tcgatgcgca gcggcgcctc cgtagcgccg tgcatggtcc gcgcgtacgt cacgccttcc
    17221 ttgaccatgg tttccatttc ctgcaagtcc tgctgcagct ttgcgccctg tggactgtcg
    17281 tccatcacgt cgacgcgcaa acgcatgcgg gtaatcggcg tttgcaggtc gtgagaaatc
    17341 gccgcgagga tctgcaagcg ctcggtcata tacatcctga tgcggtcctg catggcgttg
    17401 aatgcccgtg cggcgcgcgc gacttcgtcc ggtccgtctt cgggcaggcg ctccgccttc
    17461 aggtcgggac cgagcgtatc ggccgctcgc gccaaatggc tcaacggccg ggtggccagc
    17521 cgtaccgcca gccagcagca tgcggccagc accaccagtt gcagcaccag cacccacgac
    17581 agccacggcg acagcggcgc gccgggcatg gggcggtaat cgatcgtcag cggcgtaccg
    17641 tcactcagtt gcagatgaac ctgcaggcga tccgggtcac ccggcacggc attgaccgtg
    17701 agcggatagc gcctgccgat accatccgcg atggattgcg cgaccctctc cgagagcgcc
    17761 acatccggcg gcgcgccggc gaccccaggt cccagaacga acgtataggt gcgccgcgcg
    17821 agcctcggca accatgcggc gcgttcgtca gcgggcaaat gatcgagcag cgcgacagaa
    17881 ctggtgacct cacgttcgat gtaacccatc atcacgttat tcaggttctg gtcccgttcc
    17941 gtcatggtga gccagaccga tagcgtttgc accagcgcaa ggctgacaaa gagaatcagc
    18001 gcaagcctcg cgaacagcga acgcggccaa cgcagcaatg aactcggctt catgagtcgc
    18061 cttcgatcat ggtcacggcc gccgagaaca catagccctc actgcgcagc gtcttgatgt
    18121 aacgcggctc gcgcgccaca tcctgcaggc gctgccgcag acggctgacc agcagatcga
    18181 tcgagcggtc gaacggatcg gcctggcggc cctgggtcag attgagcagt tgatcgcgcg
    18241 tgagcacgcg ctgtggatga tcgaggaaca cgcgcagcaa gcggtactcc gcgccgctca
    18301 gggccaccat ggtgccctcg gcgtcgagca gatggcgcgc ggtggtgtcg agccgccaat
    18361 cgccaaaacc gatcatcggc gcggcttccg tcacctgcat gccgggcggc agcatgcggg
    18421 cgcggcgcag cacggaacgg atacgcgcca gcagttcgcg caccgcaaac ggcttggaga
    18481 ggtaatcgtc ggcgcccatt tccagcccga cgatgcgatc ggcctcctcg ctgcgggcgg
    18541 tcagcatcag gaccggcacc gcgcgaaact tgccggcgcg caattcgcgg cacagcacga
    18601 ggccgtcctc gccgggcagc atcaggtcga gcacgatcag atccggcgcg ccctgctcga
    18661 ggacggtgcg catctggcgg ccgttggcgg ccagcgaaac gcgcaggccg ttcttctcga
    18721 ggtaggtggc aagcagttca cggatgccgc gatcgtcgtc gacgatcagc acgtggtcgg
    18781 tgttttccat cagaggtcaa tcctttcgac gattgacgcg cacccggttc acgttgacca
    18841 gcgaacactg cacgggatgc gccagttccg ctgccaatcc gcccgccagg aaggcgagaa
    18901 tcgccatgaa ccgtctcatg cgtcctccac gacggacagg ctggcgccat tggccacgct
    18961 ggcctccagc gtatacgggt cgacgccggt gaggcaaccg atgttggcgc gccagagctg
    19021 cggatcctta cgcgtctggt ggaacggata tatgccgcag tgcttgcaga agtagtgctt
    19081 cgccgtgcgt gtattgaact ggtagagcgt cagcgcttcc tcgccttgca ggatcttcag
    19141 ttcacctgcg gcgaacggcg gcgtcatcag cgcgcctttg cgccggcaca ggctgcaatt
    19201 gcaacgcgag gcaggcagga cattggtgcg gacttcaaat ttcacggtcc cgcagtggca
    19261 ggatccttgc agcagttcgg tactcatgtg gcgctccgca gtaggcatgg ctgctttata
    19321 gcccagtccg cgcggcgctt tgtatcccaa tgtatccgtg tgctcctccg acacaaaaca
    19381 ttgcaccggc gccgcctcgg cgacacaagc cagatacgaa cgggcattta aatgcagtca
    19441 tgcccaccac gggaatgccc ggcatgacgt ctcggtctac acccggcgct tccctttttt
    19501 caaaggacgc tgacatgctc tcccgactca aaaccaccac ggccgtcgcc ctgttcgccg
    19561 caaccgtggc agccggcgcc gcggccatcg caacgctcgt tgcaagtccg gcggccagcg
    19621 cggcctctac gcccaccgca aagagggtcg acaacaacac gaccgcgccg gaattcaccg
    19681 gcatcgataa atggctcaat agcgatccgc tgacccttca gcaattgcgc ggcaaggttg
    19741 tgctggtcga tttctggacc tatacgtgta tcaactgcat ccacgtgctg ccctatgtaa
    19801 agagctggaa tcagaaatat aaggaccagg gcctggtcgt ggttggcgtc catacgcccg
    19861 aatatccgtt cgagcgcgac acggacaacg tcaggaaggc gatcaagcgc ttcgacatta
    19921 cgttcccggt cgcgcaggac aaccgctatg cgacgtggaa cgcctacggc aaccagtatt
    19981 ggccggcgtt ttatctggtc gacaagaaag ggcacgtggt ctacacgcac ttcggcgaag
    20041 gcgactacga acagaccgag gcgaagatca aggccttgct aacggaaaag tcgtgattcg
    20101 tcgcgcggct ggacgtatcg ttcatcggag atcttatgtc agacaccatc cttccccaat
    20161 cgccgtcgcg tcgtcgattt gtgagcgtgg cggcggcgac cgttgccgcg ggctcattaa
    20221 gtcagcccgc ttttgcagat cctgatcaag ccatgattca aatcaccgaa tcggacgggg
    20281 gcgtcaaaac cgctattcgt ccgcttcggg tgcatgtgcc ggaatcgcag cttatcgatt
    20341 tgcgcagacg catcaaagcg acaagatggc cggaacggga aaccgtcgcc gacgactcgc
    20401 aaggcgtgcc cctcgcgatg attcaggaac tcgcccgcca ttgggcgaca gattacgact
    20461 ggcgcaagtg cgaggcaaaa ctgaattccc tgccacagtt cgtgaccgag atcgatgggc
    20521 tggacattca tttcatccac gttcgttcga aacacgacaa tgcgatgccg ctcatcgtca
    20581 cgcacggctg gcccggctcg gtcatcgagc agttcaagat catcgatcca ctgaccaatc
    20641 ccacggcaca cggcgcgagc gcgtcggatg cttttcatct ggtcattccg tccttgcccg
    20701 gctacggatt ttccggcaaa cctacgacga cgggttgggg acccgagcgc accgcgcgtg
    20761 cctgggtcgt gctgatgaag cgccttggat acgcgcgatt tgcggcgcaa ggcggcgatc
    20821 tgggtggcgt tgtcgcgaat gtgatgggta aggaggcgcc gccggaattg ttgggcattc
    20881 acgtcaactt tccggcgacc gttccagccg ggattgccaa ggcgctgcaa gccggtgatc
    20941 caccgccgcc taacctctcg gacgacgaaa aacacgctta cgagcaattg agcagcgcag
    21001 ccatgaaacg acgcgcctat gcgctcgaaa tggggacccg cccgcaaacc ctctacggcc
    21061 tggccgattc gcccgtcgga ctggcaagct ggctgctcga tcacggcgac ggctatgtgc
    21121 agccggcagc ggcactgacc tcggccgtgt tcgggcaccc ggtcaacggc gaatccgcag
    21181 gcgagctgac acgcaacgac gtgatcgacg acatcacact ctactggctg acgaacacag
    21241 gaatttccgc ggcgcgtttc tattgggagt ctcacttcaa cttcctggcc gccgccgatg
    21301 tatccgtccc ggctgccgta agcgtctttc ctcgcgagaa ctatcaggcc ccgcgcagct
    21361 ggaccgagca ggcgtatcac aacctcatct attacaacag gctcgatcga ggcggtcact
    21421 ttgcggcctg ggaacagccg caattatttg ccgaagaagt tcgcgcgggt ttgagaccgc
    21481 tgcgccaacg tcaatgactt cccgcgaggg tcgtgcaggt caatcttcaa cgttggacgc
    21541 aaccaggcgt tacgtacgcg taatcgatgc gccgccatca accagcgacg ctgtcccggt
    21601 cacgaaagac gagtcgtcgg acgcgagata aagcaccgag cgggcgagtt cttcaggccg
    21661 cgcaacccgc ttgagcgcat gcaatccagt cacgaacgct tgcgattcgg tcgtgtcgtt
    21721 catgtcgcga tacatcgggg tgtccacggc gccgggcaga atggcgttga cgcgcacacc
    21781 ttgcggccca tactccgcgg cgagcgcctg cgtcaaacca atcaagccgg ccttgctcgc
    21841 ggcgtaggcc gcggtgcccg gaaaagcgaa cgagtagccg acgaacgtcg acgtgaaaat
    21901 gatcgaacct ccgccttgct tcagcatttc ggggatctgg tgcttcgcac ccaaaaaggc
    21961 gctcgtcaga ttggtcgcca acgcagcgga ccagccagcc tccgacacgc ccgtggtcgg
    22021 gcccatttcg cccaaagtac cggcgttgtt ataagcaatg tcaagccggc cgaagcggct
    22081 gacggcaagc tcgaccagcg ctttcgcata gtcctccgac tgcacgtcgc ccgccagaaa
    22141 cgcagcctcg ccgccttccc tgacgatctc ggcagcgagc gtttcaagct cggctgcgcg
    22201 gcgcgccgcg atcaccactt ttgcgccttc cgcggcgaac agtttcgcgg tggcacggcc
    22261 aattcccgca ctggcgccgg tcacgatggc gacttttccg tttaagcgtt gcatattcaa
    22321 tctcctgctg cagtttgctg gccagtgccc catgccccgg tccatgggaa gcaatatagg
    22381 cttcagccgc aggcgcagga aggcataaaa catgggtata ttattcagga tttatgaatg
    22441 atcggagaaa gacatggatc ggctgcgcgc ttttgaagtc ttcgtgaccg tggtgagccg
    22501 cggcagcttc actcgcgcag ccgatgcgct cgatacgtcg cccgccaacg tcacccgcta
    22561 cgtgaacgag ttggaagcgc atctcggcac gcgcttgctg aatcgcacct cacgcaggct
    22621 ttctctcacc gagggtggcg agacctttta cgcccgctgc aaatccattc tcgacgacgt
    22681 cgccgagacg gaggggctcg tgtcatccgc gtcggtcgaa ccgcgcggcc ggttgcgcat
    22741 caatgcgccg gtgagcttcg ggattctgca cctggcgccg ctatggcccg cgttcatgcg
    22801 gaagtatccc gaagtggaac tcgacgttgc gctgatcgat cgcgtcgtcg atatcgtcga
    22861 tgagggctac gacctcgcca ttcgcatctc acgcgccggt tcgactagcc acgcagcccg
    22921 caagctggcg acatcgagaa atatcctgtg tgcgtcgcca gcttacctgg cgcgctgcgg
    22981 atatccggcg gcgccggctg acttgatcga gcatcgatgc atcggctact cgtatgcggc
    23041 caccggcgac gagtggcaac tggccgacag tgagggcaag tctcacgccg tgaaggtgaa
    23101 ctgccacatg cataccaaca atggcgatac ggcacgcgca gcggcactcg ccggacaagg
    23161 cgtgatctgg cagcccacgt ttctggtcgg caacgacctg cgtgccggca cgctcgttca
    23221 ggtattgcct gcgtatcgcc tgcctgacat cgatgtactc gccttgtatc cgagccgtcg
    23281 gcacctgagc gccaagatac gtgcgatggt cgattttctg gccgaagcgt tcggcaacgt
    23341 gccgccgtgg gatcgggctg acggagattg agcgcggacc tcgttgaccg aggcggccgg
    23401 ttgcctggtg tgagcgggac gttcacccac ctgttggaac agaccgctgc agggccgaat
    23461 ttgctcggca cactaaaccg ttgcccacgc tcaccgcgca gcacgaagct cggacggctc
    23521 cagatagaaa ttcctgttcc ccgcgaggcg gccgaccata aaatcgatga acgtcctcac
    23581 ccgcagcggc tgctcggttc gatgacgata gtagatgtag attgcctcac gctgcgtaac
    23641 atgcttcacg agcagcggaa cgagttggcc acgcctgatc ggcagggtcg ccgaaaagct
    23701 gccgagttga ccgatgccga gtccggccag cacggcctgg gtctccgcgt ccgtgtcatt
    23761 gacgcataag gccgtcgcga cgtcacgata gacgatctcg tcaccgatca ggaattccca
    23821 cggagcctgc ttgcccgtat ttgcccgccg atagccggtg caacggtgcc catcgagctc
    23881 gtcaatggtc cgcggcgcgc catgccgctc gatatagcca ggcgacgcgc agacgatcag
    23941 ttgaatcggc aagagccgtc gcgcgatcgt gccgcccgac ggcggagaac caccccgaaa
    24001 gcctacgtcg gcgcgatcgc tgacgaggtc ggtgaagtgg tcgtcgaact gcacatccac
    24061 ttgcacgttt gggtgcaatt cggcgaattc caggaaatat ggccacagca ccgtgtgccc
    24121 gaccgctctg ggtgcgctca cccgcagtgg ccccgcaatc tcgtctttcg atcgccgcgc
    24181 gtcgtcgagt gccgacgaca aaatggccag cgcaggtttc acgctatcaa gcagcccctg
    24241 cccctcctcg gtcaagctca gcttgcgtgt ggttcgatga aacagccgaa cacccaattc
    24301 cttttccagt tgcatcaccg cgtggctcgc ggcctgcggc gagataccct ggtccacggc
    24361 cgccttgcgc aagcttccga gcgccgcggc ccggacgaat atcgtaatcg cgcgaacttc
    24421 gttcatggcc gctcggattc gcaaataaaa attgattatc agtctaccag gcggcgtcta
    24481 gtttgggcca atcgcttctc ctaatattcg ttcactttca ctcgaaacgt aggagaaaca
    24541 ccatggtcgg caacacgaaa aatggcttca agcgcgtgtg gttcatcacc ggcgcgtcgc
    24601 gcggcctcgg cgcgctgatc gcccaggcgg cgctggccga cggcaacgcg gtcgttgccg
    24661 ccggccgcaa tgtggcggcg atcaccgagc gcttcggcaa ttcgccggcg ctcctccccg
    24721 tggcattgga cgtgacaaac gaagcgcagg cgaaggctgc ggttcaagcg acggtcgaac
    24781 gattcggccg gatcgacgtg ctggtcaaca acgcgggctt cggtttgctt ggcgcgatcg
    24841 aggaatccag cgacgccgat gtgcggcgca tgtacgacac gaacgtgttc ggcctgctga
    24901 atatcacgcg cgccgtgctg ccggtgatgc gcgcgcagcg ggccggccac gtcatcaata
    24961 tgtcgtcgat cggcggctat tggtccgccg ccgggttcgg cgcgtactgt tcgaccaagt
    25021 ttgccgtcga aggcttgacc gaggcattgc gtaccgaact gaagccgctc ggcatccacg
    25081 ccacggtcgt ggagcctgga tacttccgca cggacttcct ggacgcgtcc tcgctcgtgg
    25141 ttgccgggaa cgtgatcgcg gactatgacg agacgtcggg aaacgttcgc cgggtcgcga
    25201 ccagcctgaa ccacaaccag ccgggcaacc cggagaagct cgcggccgcc atgatcgaac
    25261 tggtcgacgc acaggcacct ccgctgcggc tgccgctcgg caccgatacg ctgaaggcca
    25321 tcgccgaaaa gaatgcctat gtcacgcagg aaaccgaaac ctggcgggca ttgtccgcat
    25381 cgactgactt ttccgcctga gcgcttgcag atcggccacg cggcggccgc gtctagacac
    25441 ccgccagcgc cggcagctcg gcctcgtacg agacgcgctc gcgccggctg ctcatccgcg
    25501 actgatgcgc caggtggtcg gcgtggctcg gttgcggcgc gttgtcgacg cgcgtcggca
    25561 agcacgcagg gtagcgagcg ttttttctct cgagaatgac ccggctcaga aagccgacgc
    25621 tataggcttt cagcaaatgc gcgtggtgcg cttcgatgta ggcggcaatc cgcgcctcct
    25681 caccgtcgat cgcggctagc tgcgccagcg tgcgcgcgtc ccaacggaag cgtaggccga
    25741 gttcgtcgaa cgcgcagttg taggcgcaca gttgcgcttc tatctccgcg tcatgggcat
    25801 tgttgcaagc atcgaactgg gacgtggtca tggtcgcctc cttcaggcaa atggcatgga
    25861 ccaagaatag gagcgcgcat gaataaacaa tagttaaagt tttttcgaaa aacgataatg
    25921 ttttgtttat acatcgatct tcccgctacg gcggcgggga aattcggatg cccaagcaac
    25981 ctgcctcaat gcgccccccg cgcgactatc tgctccgcga gacacagttc atcggaccgc
    26041 gcgctaaaga cccaggcacc cccaactcct cgaaaacttt caattgacac acagcccttc
    26101 gcgcccagcg aattcaaaac catagagggc aaaccctatc tattagggat accgaataga
    26161 aatcataaaa tgttttaact ttcgcttatc gctgttctcg cgcagcatcc tctcagacgc
    26221 gctccacgcg aagcatttct tacccggttg ttgcgcgaac aacgagtaag ggttttcgtt
    26281 agtgcaagtc ccgctgaccg accaatggcg cttcgttgcc cacagaaaac ccggcgatag
    26341 agcgattcga tcacgtaaat ccaacgtgcc gttcgagcac gacggaatct acaggagacg
    26401 agagacatgg gacattccaa cgtacccgga agaagcagtg caacattcag gcaaaaccgc
    26461 tgggtgcaac tggcgatcgg catcgtatgc atggggctcg tcgcgaatct gcagtatgcg
    26521 tggacgctct tcgtcgtacc gatggacaac gcccaccatt gtggccaggc ggccatccag
    26581 accgcgttca cggtcttcat cgtcacggaa acctggctcg tgcccattga aggctggctc
    26641 gtcgacaagt tcgggccgcg cccggttgtg atcggcggtt cgctgtgcgc ggcgctcggc
    26701 tggatcatcg acgcgcatgc gagcagtctc gtcgagctct acatcgcagc ggtgatcgcc
    26761 gggatcggcg cgggttgcgt gtacggcacc tgcgtcggca cggcgctcaa atggtttccg
    26821 gacaagcgcg gtctcgcggc cggtttgacc gcggcgggtt tcggcgccgg tgcggccgtt
    26881 accgtgattc cgatcgccaa catgatccag cgctcgggct acgagcacac cttcatgttc
    26941 ttcggcattt ttcagggcgt ctgcattctg ctgctcgcca ccatgctggt acggccgaag
    27001 ccgcccgcca ccgcagtcgc ggccaaacgc gtggtctcga gcaagatcga ctacacgccc
    27061 ggcgagatga tccgttcgcc gctgttctgg gtgctgtacc tgatgttcgt gttcgtcgcg
    27121 gccggcggca tcattgcgac cgcgcagctc gggccgatcg cgaaggaata cggctttgcc
    27181 aagatgccgg tgaatctgct gggcatcacg ttgccgctgc tcacgatgac gctgtcgatc
    27241 gacaacctct gcaacggctt cacgcgtccg ttgtgcggtt ttctgtcgga ccgcatcggc
    27301 cgcgaaaaca ccatgttcat gatctttctc ggcgaaggcg tcgcgctgct cgggctgatg
    27361 cagttcggcc acaacccgta cgcattcatg ttcttcgcgg ccgccgtgtt cctgtgctgg
    27421 ggcgagatct tctcgatctt cccggcgacc tgtgcggaca cgttcggcag caaatacgcc
    27481 gcggccaatg cagggacgct ctataccgcg aagggtacgg cgtcgatgct ggtgccgatc
    27541 gcatcggtgc tgtccgcgaa cggcggctgg agcaccgtgt ttatctcggc ggcggtggtg
    27601 tcgatcgcgg ccgccgtgtc cgcgaaattc attctcgccc ccatgagaaa gcgctggatc
    27661 gagaactcgg gcgcgccagc cggcgcggtc ctcgccgaag cgcggctgca accgtcgtcc
    27721 agcaccccat ctgaataaat aacaacgcga gcgcgtagct cacgacaaac cccataacgt
    27781 cccgcgacca aggagagaca tcatggcaga agccgacagc cccacaacag aaaataagct
    27841 gaagcagcag gcaaccgaga cgaccgacgg attccatctc gtcatcgatg cgctgaaact
    27901 gaacgacatc gacaccatct tcggactggt cgggattccc atcacggacc tggcgcgcct
    27961 tgcacaagcg gaagggatgc gcttcatcgg ctttcgtcat gaacagcacg cgggcaacgc
    28021 agcggctatc gccggcttca tgacgcaaaa gcccggtatc tgcctgacgg tgtcggcgcc
    28081 cggctttctg aacggcttga ccgcgctcgc caacgcgacc accaactgct tcccgatgat
    28141 cctgatcagc ggctcgagcg agcgcgagat cgtcgatctg cagcagggcg attacgaaga
    28201 aatggatcag ctgaatgcgg ccaagccgta cgcgaaggcc gcatatcgcg tgctgcatgc
    28261 ggaagacatc ggcatcggcg tggcgcgcgc gattcgtgcc gcggtgtcgg gtcgtccggg
    28321 tggcgtgtat ctggacttgc cggccaaact gctcgcgcaa acactggatg ccgtaaaggc
    28381 gcagcagtcg ctgatcaaag tggtcgacgc cgcgccgcgt cagctgccgg cgccggaatc
    28441 ggtcaagcgt gcaatcgacg tgctgaagag cgccaagcgt ccgctgatcc tgctcggcaa
    28501 gggcgcggcg tacgcgcagg ccgatgcgga gattcgcgcg ttcgtcgaac aaagcggcat
    28561 tccgtatctg ccgatgtcga tggccaaggg cctgttgccc gacacgcacg cgcaatcggc
    28621 ctcggcggcg cgctcgttcg tgctgcagga agccgacgtt gtcgtgctga tcggcgcgcg
    28681 gttgaactgg ctgctctcgc acggcaaagg caaaacgtgg ggcgcggcgc ccaagcgctt
    28741 cgtgcaggtc gatatttcgc cgaccgagat cgacagcaac gtcgcaatcg cggcgccggt
    28801 gatcggcgat atcggctcgt gtgtggcggc actgcgtgcc ggtctcgatg cgggcttcgc
    28861 gaaaccgagc accgaatgga ccggcgcgat cgccgagcgc aagagcaaga acctcgagaa
    28921 gatggccgcc acgctcgaca agaacccgtc gccgatgaat ttccacagcg cattgcgtgc
    28981 gattcgcgat gtactgaaga cgcgcccgga catcaacgtc gtcaacgaag gcgcgaacac
    29041 gctcgactac gcgcgcagca tcatcgacat gtacgagccg cgcaaacgct tcgattccgg
    29101 cacgtgggga atcatgggca tcggcatggg cttcgcgatc ggcgcggcgg tgacgagcgg
    29161 caaaccggtc gtcgcgatcg aaggtgacag cgcgttcggc ttcagcggca tggaactcga
    29221 aaccatctgc cgttacgagt tgccggtttg cacgatcgtg ttcaacaaca acggtgtgta
    29281 tcgcggcacc gatgtgaacc cgacgggcgg caaggacgtt gcgccgaccg tgttcgtgaa
    29341 gggcgcgcgc tacgacaaga tgatcgaggc attcggcggc gtcggccacc acgccaccac
    29401 accggaagag ctgacgaagg cgctgctgga agccatcgca tcgggcaagc cgaccttgat
    29461 caatgccgtc atctatgaag cggccggtac agaaagcggc cgcctgacca atctgaatcc
    29521 gcaaagcgcg gcaatgaaaa agtaatccag agcaaccacg gagatatcaa atgaccaaac
    29581 cgctcgaagg catcaagatc atcgacttca cccacgttca ggccggtccg gcatgcaccc
    29641 agttgctcgc gtggttcggc gccgacgtga tcaaggttga acgaccgggt tcaggcgacg
    29701 tcacgcgcaa ccagttgcgc gacattcccg acgccgacgc gctgtacttc acgatgctca
    29761 acagcaacaa gaagtcgctg acgttggaca cgaaaaagcc cgaaggcaag gaagtcctcg
    29821 aaaagctgat tcgcgaatcc ga
//
LOCUS       DATASH010000034         3047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6531715,
            whole genome shotgun sequence.
ACCESSION   DATASH010000034 DATASH010000000
VERSION     DATASH010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6531715"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..914
                     /locus_tag="VHV99_01175"
     CDS             48..914
                     /locus_tag="VHV99_01175"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX3634102.1"
                     /translation="MNELSTRPARPQCAEAAPQTRWPHTGYPAMLTATAAWHAFVLAG
                     WLLTPALWPWWLAAILANHVVFTVAGLLPRTSLLGPNWTRLPAGARNADAIALTIDDG
                     PDPVVTPQVLDLLDALSVRATFFCIGAKAQRYPALIREIVARGHALENHSQVHLHTFS
                     LTFPAALTREIDAAQRTFETLSGERPMFFRAPAGLRNIFLEPVLRKLDLRLAAWTRRG
                     YDTRERDPHVVARRLLDGLAARDILLLHDGNAALTVEGEPLILAVLPRVIDAARRQHL
                     RFVTLREARIAH"
     gene            complement(1035..2447)
                     /locus_tag="VHV99_01180"
     CDS             complement(1035..2447)
                     /locus_tag="VHV99_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HEX3634103.1"
                     /translation="MKASTIAEREARGRAAREHSKRSSHRTVGELNRNPIELLKQSSE
                     ERVTNLVPLRYGRMAVSPFTFFRGTAILQAHDLSRVHDSGLTMPICGDGHLMNFGGFA
                     TPERQLVFDLNDFDEVSTGPFEWDLKRLTASLVVAARHMRLSRGTAEHLVMTAVTEYR
                     DRMAQYAQCGALELWYDRITFDRMAETALTPERRRTVRRGMEKAASRTHENLLEKMAV
                     RDGDHWTIRDAPPALFHVLGANTLFTPEEAEVFKAANWRKLLERMWGEYLKTMSHDRR
                     ELLSHFTVQDIVFKVVGVGSVGTRCMVVLSVDHMGKPLFLQIKEARPSVIAQFYKSPG
                     NKHEGERVVQGQRMLQAASDIFLGWATGPQERHFYFRQLRDMKLSANIELFDTDLLEG
                     YAQLCGWIMARAHAKASGQAIEVSAYIGRGDQFAEALTAYANTYADQVERDYDVFLKA
                     CRSGELEARSDEDMAADFRV"
     gene            2626..>3047
                     /locus_tag="VHV99_01185"
     CDS             2626..>3047
                     /locus_tag="VHV99_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HEX3634104.1"
                     /translation="MRPAPSQQHEPELPLHTPSVPSVTLAAIIAVIAWISIAAQTDLT
                     IDRTLARGLSVLDGIARLSSYLTNLTVLACAICFTCVALRGRHSPIARFFRQPTVVTA
                     VVVYMAFVGIAYNLLLRGLWSPAAYRLLLNESMHSVLP"
BASE COUNT          533 a         1013 c          889 g          612 t
ORIGIN      
        1 ttcaaggcct tccctggaag gaggaaggaa gacaggaagc acgctttatg aacgaattat
       61 ctacacggcc tgcacggccg caatgcgccg aggccgcgcc gcagacgcgc tggccgcaca
      121 ccggctatcc ggcgatgctc acggccaccg ccgcatggca cgcattcgtg ctggccggat
      181 ggctgctgac acccgcctta tggccttggt ggctggccgc gatcctcgcg aatcacgttg
      241 ttttcacggt ggccggcctg ttgccgcgca cctcgctgct gggaccgaac tggacgcgac
      301 tgcccgcggg cgcgcgcaac gcggacgcca tcgcactgac catcgacgac ggtcccgatc
      361 cggttgtcac accgcaagtg ctcgatctgc tcgatgcttt gagcgtacgc gccaccttct
      421 tctgcatcgg tgcgaaagca caacgctacc cagcgctcat acgggaaatc gtcgcacgcg
      481 gccatgcgct ggaaaaccat tcgcaggttc acctgcacac gttttcgctc acgtttccgg
      541 cggcgctcac gcgcgagatc gatgcggcgc agcgcacctt cgagacattg agcggcgagc
      601 ggccgatgtt cttccgcgcc ccggcgggat tgcgcaacat cttcctcgaa ccggtgctgc
      661 gcaaactgga tctgcggctc gcggcgtgga cacgtcgtgg ctacgatacg cgcgagcgcg
      721 atccgcacgt ggtcgcgcgc cggctgctcg acggtcttgc cgcgcgcgac atcctgctgc
      781 tgcacgacgg caatgccgca ctcacggtcg aaggcgaacc gctgatactc gcggtcctgc
      841 cgcgcgtgat agacgcggct cgccggcagc acttgcgctt cgttacgctg cgtgaagcac
      901 gtattgcgca ttaggctcga gccttcgcct cgagccccag acccgagcac tgaactgaac
      961 ctcgaacagg gccacgccgt gctcacggct cgccaggcgg gccagcaagc gagccgcgca
     1021 aacaccgccc gcggttacac gcggaaatcc gccgccatgt cttcgtccga acgcgcctcg
     1081 agctcaccgc tgcggcacgc cttcaggaac acgtcgtagt cgcgttcaac ctgatccgca
     1141 tacgtgttcg cataggccgt gagcgcttcc gcgaattgat cgccacggcc gatatacgcg
     1201 ctgacttcga ttgcctgccc gctcgccttg gcatgcgcgc gcgccatgat ccagccgcat
     1261 agttgcgcat agccttccag caaatcggta tcgaacaatt cgatattcgc cgacagtttc
     1321 atgtcgcgca gttggcggaa ataaaagtgc cgctcttgcg gcccggtcgc ccagccgaga
     1381 aagatgtcac tcgcggcctg caacatacgc tgcccctgca cgacacgctc gccctcgtgc
     1441 ttgttgcccg gcgacttgta aaactgcgcg atcaccgacg gccgggcctc cttgatttgc
     1501 aggaacagcg gcttgcccat gtgatcgaca ctcaatacca ccatgcagcg cgtgcccacg
     1561 ctgccgacgc ccaccacctt gaacacaatg tcctgcacgg tgaaatggct cagtagttca
     1621 cgcctgtcgt gcgacatcgt cttgaggtac tcgccccaca tgcgttcgag cagcttgcgc
     1681 cagttggccg ccttgaacac ttcggcctct tccggcgtga acagcgtgtt cgcacccagc
     1741 acgtggaaca gcgccggtgg cgcatcgcgg atcgtccaat ggtcgccgtc acgcaccgcc
     1801 attttctcca gcaggttttc gtgggtacgg ctcgcggcct tttccatgcc gcgccgcacg
     1861 gtacggcggc gctcgggcgt cagcgcggtt tccgccatgc ggtcgaacgt gatgcggtca
     1921 taccagagtt cgagcgcgcc gcattgcgca tattgcgcca tgcggtcacg gtattcggtg
     1981 acggcggtca tcaccagatg ctcggctgtc ccgcggctca accgcatatg ccgcgcggcg
     2041 accacgagac tcgcggtcag ccgtttcagg tcccattcga aaggaccggt cgagacttcg
     2101 tcgaagtcat tcagatcgaa tacgagttgc cgctccggtg tggcgaaacc gccgaaattc
     2161 atcaggtggc cgtcgccgca gatcggcatg gtgaggcctg aatcgtgaac ccggctcaga
     2221 tcgtgagctt gcaggatcgc cgtaccgcga aaaaacgtga aaggcgacac ggccatgcgc
     2281 ccatagcgca gcggcaccag attcgtcacg cgttcctcgc tgctttgctt gagcagctcg
     2341 atgggattgc gattcaattc ccctaccgta cgatgactcg aacgtttcga atgttcacgg
     2401 gcggcacggc cacgggcctc ccgttcggcg atggtgctgg ctttcatgct cttctccggt
     2461 ttttgtgtga atttttactc ggttctagaa gactgcttct gcgggcacgg taaggcagcg
     2521 ctcatgctag cagaccaccc gtttgtcgtg agcaatccgg cctgggcggg cgcgattttg
     2581 tcgcgcttgc tgcaaagcaa atccggtatg tataaaatcg tttacatgcg ccctgcccct
     2641 tctcagcaac acgagcccga gctcccgctc cacacgccca gcgtcccatc cgtgacgctg
     2701 gcggcaatca ttgccgtcat cgcgtggatt tcgatcgccg cacaaaccga tctgacaatc
     2761 gatcgcacgc ttgcccgcgg gctcagcgta ctcgatggca ttgcgcggct aagcagctat
     2821 ctcaccaatc tgacggtgtt ggcttgcgcg atctgtttta cctgcgtagc gctccgcgga
     2881 cgccattcgc cgatcgcgcg ttttttccgc cagcctaccg tggtgaccgc ggtcgttgtc
     2941 tatatggcgt ttgtggggat cgcgtataac cttcttctgc gggggttgtg gtcgcccgcg
     3001 gcctaccggt tgctgctcaa cgaatcgatg cacagcgtgt tgcctat
//
LOCUS       DATASH010000035         2327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6532001,
            whole genome shotgun sequence.
ACCESSION   DATASH010000035 DATASH010000000
VERSION     DATASH010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6532001"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VHV99_01190"
     CDS             complement(<1..587)
                     /locus_tag="VHV99_01190"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3634105.1"
                     /translation="MKIERYPPFNVLQSLLVVARCGNFTAASQQLFLSQSAVSRHIQQ
                     IEEYFGCALFVRHTRMVVPTAECQRLVPIVEELLATARRSMHIISTGGQTITLRVTPT
                     LAARWLLPRLPDFYAEVPGTQLNIDTAWFLPTNFGEGTLDAAILFGNGRWEGLEATSL
                     MRERLTPVCSPQTAHGPHPLAEPADLKHHTLLHSS"
     gene            780..2243
                     /gene="argH"
                     /locus_tag="VHV99_01195"
     CDS             780..2243
                     /gene="argH"
                     /locus_tag="VHV99_01195"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEX3634106.1"
                     /translation="MTQHSDRLWGGRFKTGPAPALTALSRSDPSFFRLAPYDLAGSRA
                     HAQELGRAEILTPDELQQLLSEMDRLEAQFLAGEVGPTLDDEDVHTFLERELTQRLGA
                     TGGKLRAGRSRNDQAANDLRLFLRDKARTLTQAVIDLQNALIEQANAHVHSVSAGFTH
                     LQPAQPIVFAHHLLAHAQSIYRDVDRLVDWDRRSARSPLGAAALAGSAICRRPELSAA
                     ELGYDGPCENSIDAVASRDHVAEFVFIASMLSVNLSRLSEEVILWTSRQFGWVVLDDG
                     YATGSSIMPQKKNPDIAELTRGKAGRLIGNLSGLLATLKSLPLAYNRDLAEDKIAAFD
                     CIDTLELVLPAVAGMIRTMRVNVDEMRRQAPLGFTLATEVADWLALRGVPFSEAHEIT
                     GALVQVCEEQGIGLAEVSAATLAKVDARLEPAVLEHVTLDAAVAARSGYGGTAPVRVA
                     EQITRLQTALNKQLAWATSYGGPTRSARAARTRRPCS"
     gene            2200..2327
                     /locus_tag="VHV99_01200"
                     /pseudo
     CDS             2200..2327
                     /locus_tag="VHV99_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006029671.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
BASE COUNT          451 a          707 c          736 g          433 t
ORIGIN      
        1 aagctggaat gcagcagcgt gtgatgtttc agatctgccg gctctgctaa tggatgcgga
       61 ccatgcgcgg tctgcggtga acacacgggc gtcaatcgtt cgcgcatcag gctggtcgcc
      121 tcgagtcctt cccatcgtcc attgccgaac aggatcgccg cgtccagggt gccttcgccg
      181 aaattggtcg gcaggaacca cgcggtgtcg atgtttaact gcgtgcccgg cacctcggcg
      241 tagaagtcgg gaagacgcgg cagcaaccag cgcgcggcca atgtaggcgt gacccgcagt
      301 gtgatggtct ggccgcccgt gctgatgata tgcatcgacc ggcgcgcggt cgcaagcaac
      361 tcctccacga taggcacaag ccgctggcac tccgccgtcg gtacgaccat ccgcgtgtgc
      421 cgcacgaata atgcgcagcc gaaatactcc tcgatctgct ggatatgacg gctcaccgca
      481 ctctggctga ggaacaactg ctgcgatgcc gccgtgaaat taccgcaacg ggcgaccacc
      541 agcagcgatt gcagcacatt gaaaggcggg tagcgttcga ttttcattta tgcaagttgt
      601 gcatggtaac catgagattt taccgtttgt cggctggaaa ttggcgttca taaaatcggc
      661 tcgaaagtta aggcttcttg tccgctggaa agcgcgagaa gcctttcaac gagcaggatg
      721 ccgagcgaca caccctcaac aaacgcgcat tcgaccatcc acttccaggg agtttttgca
      781 tgacccaaca ttcagaccgg ctgtggggtg gacgcttcaa gactggcccg gcgcccgcgc
      841 tgaccgcgct gtcgcgctcc gacccgagct tcttccggct agcgccctat gacctggcag
      901 gctcacgcgc gcacgcgcag gagcttggac gcgctgagat cctgacgcct gacgagctgc
      961 agcaactact aagcgaaatg gaccggctgg aagcgcagtt cctggcaggc gaggttggcc
     1021 cgacgctcga cgatgaagac gtgcatacct tcctcgaacg ggaactgacg caacgcctgg
     1081 gcgcaactgg cggcaaattg cgcgccggcc gttcgcgcaa cgaccaggcg gcgaacgacc
     1141 tgcgcctctt cctgcgcgac aaggcgcgta cgctgaccca ggcggtcatc gatttgcaga
     1201 atgcattaat cgaacaagcg aacgcgcatg tgcatagcgt gagtgcaggg ttcacgcatt
     1261 tgcagccggc gcaaccgatt gtgttcgcgc atcacttgct cgcccatgcg cagtcgatct
     1321 atcgcgatgt cgatcgcttg gtggattggg atcgccgcag cgcgcgttcg ccattgggcg
     1381 cggcagcgct ggcggggtcc gcaatctgcc ggcggccgga gttgtcggcg gcggaactcg
     1441 gctacgacgg cccgtgcgaa aactccatcg atgcggtcgc ttcgcgcgac catgttgcgg
     1501 agttcgtgtt catcgcgagc atgttgtcgg tgaacctgtc gcggctctcg gaggaagtca
     1561 ttctctggac ttcgcgccag ttcggttggg tcgtactcga cgacggttat gcgaccggca
     1621 gctcgatcat gccgcagaag aaaaacccgg atattgcaga gcttacgcgt ggaaaggccg
     1681 gcaggttgat cggcaacctt agcggcttgc tcgcgacgct caagtcgctg ccgctcgcct
     1741 ataaccgcga tctggccgaa gacaagatcg cggcgttcga ttgcatcgat accctcgaac
     1801 tcgtgcttcc cgccgtggca ggcatgatcc gcacgatgcg cgtgaacgtc gatgaaatgc
     1861 gccgtcaggc gccgctcgga ttcacgctgg ccacagaagt tgccgactgg ctcgcgctgc
     1921 gcggcgtgcc gttcagcgaa gcccatgaga tcaccggcgc attggtgcaa gtctgcgaag
     1981 agcagggtat cgggctcgcc gaggtatcgg ccgcgacgct ggcaaaagtc gacgcacggc
     2041 tggaacccgc ggtgctggag cacgtaacgc tcgacgcagc cgtggcggcg cgcagcggtt
     2101 acggcggcac cgcgccggtg cgtgtagccg agcagatcac gcgtttgcag acggcgctca
     2161 ataagcaatt ggcatgggcc acgtcatacg gtggaccgac aagaagcgcg cgcgctgctc
     2221 gaacgcgtcg gccttgctca taagtgtgat gcgttcccgg tggaactctc gggtgggcag
     2281 caacagcgcg tcgccattgc acgggcgctt gccatgcatc cccaact
//
LOCUS       DATASH010000036         6243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1587032,
            whole genome shotgun sequence.
ACCESSION   DATASH010000036 DATASH010000000
VERSION     DATASH010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6243
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1587032"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..576
                     /locus_tag="VHV99_01205"
     CDS             58..576
                     /locus_tag="VHV99_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016354790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3634107.1"
                     /translation="MSFDTDLVGVLPQLRRYARALTGDRAWADDLVQDVTERALGRSK
                     GFRVGSNLRAWLFTIMRNLYIDQLRGRRDIAVDDETAPWRQMAAPVGEVDGLVLRDVQ
                     RALYCLPVEQREVMLLVCVEDMSYQEASVVLNVPAGTVMSRLSRAREHMRVLLGEEPG
                     RKSASLKIVSGT"
     gene            573..1367
                     /locus_tag="VHV99_01210"
     CDS             573..1367
                     /locus_tag="VHV99_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016354791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX3634108.1"
                     /translation="MTNHERPEQAESSAAADELRALSAFIDDEAPEAERAETAARIAR
                     DPASAATVAAYRAQDNALRALFAATADKEPQVVVVRPRKRYLMAASWLVVGIACGFLM
                     HMLLPVLGQERPAPTFAERADIAYAVYAPEQRHAVEVAASQEDHLVTWLSKRLNRTLT
                     IPSLHEYGFELVGGRLLPGEDGPAAQFMYQNANGERLTLYMTGGSGKHKDEYAIRMLR
                     EGSRRTFYWTTDHAGYALSGQIGEAKLRAISFDTCAALGGDPKKWQ"
     gene            1541..2038
                     /locus_tag="VHV99_01215"
     CDS             1541..2038
                     /locus_tag="VHV99_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007589134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEX3634109.1"
                     /translation="MTIAASADERFTLSSPSLADNATLDSRHAASAQDCGGKNISLPL
                     AWSNAPTDTKSFAVTIYDPDGARGLGIVHWILYGIPAATTALAEGGPPPAGSIGGINR
                     TGKDGYYGPCPPHGDAPHHYVVQAYALDLAANALPAALDRNALIAAMKGHVLASSSTV
                     VRYAR"
     gene            complement(2099..3550)
                     /locus_tag="VHV99_01220"
     CDS             complement(2099..3550)
                     /locus_tag="VHV99_01220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3634110.1"
                     /translation="MKRTLEEALAQASPRLAPRPITVASVLIHGGVVVLWLVLFARAF
                     FLHGVVAWSTGIAYVVYDTLLLMFVAAKSWTLVRPQKPLDRKQRERGLPPLGVIVASH
                     NEAAVLPNTLAKLLGQTHGAAQIVIADDGSSDATAELLTQRFGLVAPPEGELSAASTL
                     HPTLFWLRVAHGGKARALNAAIEKISTPIVMTVDADTRLADDAVLAMRSAFALQSNLV
                     AAAGILVPVCSKTMSGRFFQWFQTYEYIRNFIARFAWMRADSLLLISGAFASFRRDAL
                     LAVGGFDPQCLVEDYELIHRLRRHSVDHGLDWDVRVVGNAHAITDAPSSFSGFMRQRR
                     RWFAGFLQTQYWNRDMTGNRRYGTLGLLMLPVKAFDTVQPIYGLTAFALLLGFIVDRR
                     GAIVLPIFSVIGAKILIDFAFHLWTVHLYRRWTGDTAGSSLWMAVVTAIVEPFTFQLL
                     RHTGAALGWVDFLGGSRAWRVQTRAGLAAADEA"
     gene            complement(3616..4218)
                     /locus_tag="VHV99_01225"
     CDS             complement(3616..4218)
                     /locus_tag="VHV99_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEX3634111.1"
                     /translation="MSTQAANVVSEPPQTEMPLRYTRTAMVLHWLIALLIICNVLLGL
                     TAESFPDSWIRPVVDTHKSIGITVLGLVLLRILWRVSHRPPPLPKAFPKWEHMAAHIA
                     HFVLYLLMIGLPLSGWLHDSAWKDAATHPMHLFGVIPWPRVGFVMNLDPALKESLHDR
                     FGALHTWLGYALYALLAMHVGGALKHQWIDKKSVISRMVP"
     gene            complement(4391..5347)
                     /locus_tag="VHV99_01230"
     CDS             complement(4391..5347)
                     /locus_tag="VHV99_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX3634112.1"
                     /translation="MELRHLRYFLAVAEERQFTRAAARLHIQQPPLSQQIQELERELG
                     FALFTRLPRGVELTSAGAAFVVDARAVLDALDQGVVNARRVANGQLGSVRIALTSSAA
                     FHPLATAAIRRFRATHPGIAIDLNEINAADIIERMMNGRVDSGILRKPIEMPAELRFD
                     LLHEERMVLVLPVGHKLLPVKGVRPGKQPRVPLKALVGESFIFVRRPGAPGMYADFIR
                     ACEEAGFKPEVVSEVPRMLSAINLVAAGGGITLVPASMQRYQQETVVYCAVAGDEAFS
                     APLHLVTRRDAANPAALQFAQAVLAFVSPKGAESPDTSHHLT"
     gene            5465..>6243
                     /locus_tag="VHV99_01235"
     CDS             5465..>6243
                     /locus_tag="VHV99_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013690046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3634113.1"
                     /translation="METTFPPNSAEHAPASLASLSKAQIRRAVLASVIGNGLEWFDFL
                     IYGYFAKIIAHVFFPVGNTALSTTLTLGTFAVGFIVRPLGGIAIGAYADRVGRRKTLS
                     MLILLMAASTLLMGLTPGYSTIGIAAPLLVLLARVMQGLSVGGEFATAAAMLTEYAPR
                     GKKMFFGSFQMTSQAVALVLSSAFGYFLTSNLDRASLESWGWRVPFLVGALVGPVGFY
                     IRHKVAESPEFVELRARLGHAPRQTIRGFFRQRGDASLCAMG"
BASE COUNT         1246 a         1933 c         1940 g         1124 t
ORIGIN      
        1 cgtcacggga acagtggccc ggagcgctga aagtgaacca gtcatgagca aaggccgttg
       61 agcttcgata ccgatctcgt tggggttctc ccacagttgc ggcgttatgc gcgcgcgcta
      121 acgggagacc gtgcgtgggc cgatgacctc gtgcaggatg tgaccgaacg ggcgctcggc
      181 cggtcgaaag gatttcgtgt cggcagtaat ctgcgcgcgt ggctgttcac gatcatgcgc
      241 aatctatata tcgatcagtt gcgcggccgg cgcgatattg ccgtcgacga tgaaaccgcg
      301 ccctggcggc agatggccgc gcccgtgggc gaagtcgacg ggcttgtgct gcgcgacgtg
      361 caacgcgcgc tgtattgctt gccggtcgag caacgcgaag tcatgctgct cgtctgcgtg
      421 gaggacatga gttatcagga ggcatccgtg gtgctgaacg tgccggccgg aacggtgatg
      481 tcgcgactat cgcgggcacg tgaacacatg cgcgtgctgc tcggtgagga accgggccgc
      541 aaatcggcat cgttgaaaat cgtgagtggc acatgacgaa tcacgaacgt cctgaacagg
      601 cggaatccag cgcggcagcc gatgagttgc gtgcgctgtc ggcgttcatc gacgatgaag
      661 cgccggaggc cgagcgcgcc gaaaccgccg cacgcattgc ccgcgatccg gcgagcgcgg
      721 cgacagtggc cgcgtatcgc gcgcaagaca atgcactgcg ggcgctgttt gccgccacgg
      781 ctgataaaga gccgcaagtc gtggtcgtgc gtccgcgcaa gcgttacctg atggcggcaa
      841 gctggctggt ggtggggatc gcatgtggct tcctgatgca catgctgtta ccggtcctcg
      901 gccaggaacg cccggcgccc acattcgcgg aacgcgcgga tatcgcttac gccgtgtatg
      961 cgcctgagca gcggcacgcg gtcgaagtcg cggcgtcgca ggaagatcat ctcgtgacct
     1021 ggttgtccaa gcggctcaac cggaccctca ccattccctc gctgcacgaa tacgggttcg
     1081 agctggtggg tggccggttg ttgcccggcg aggacggacc cgcggcgcag ttcatgtatc
     1141 agaacgccaa cggcgaacgg cttacgctgt atatgactgg cggttcgggc aagcacaagg
     1201 acgaatacgc catacgaatg ctgcgcgagg gctcgcgacg aactttctac tggaccacag
     1261 atcacgcggg ttacgcgctc tcgggacaga tcggcgaggc caagctccgt gccatcagct
     1321 tcgatacctg cgcggcgtta ggcggcgatc caaagaaatg gcagtgagaa gttatcagga
     1381 tcgctgcgct tgaagaaggg gaacacatcg agcacacgtg gtacgcagag cgaagttatt
     1441 tagacccaag accagaagaa caatgaaaaa tgatcgaagc agcatatgcc gcgcgaagcg
     1501 gcttttgtgc gtggccacga taggcgcggg atgttttttc gtgaccattg ccgccagcgc
     1561 cgatgaacgc ttcacgctga gttcacccag tctcgccgat aacgccaccc tcgattcccg
     1621 gcacgccgcc agcgctcagg attgtggcgg caagaacatc tcgttgccgc tcgcgtggag
     1681 caatgcgcca acggacacga agagcttcgc ggtcactatc tatgatcccg acggcgctcg
     1741 cggcctgggc atcgtgcatt ggattctcta tgggattcct gctgcgacga ccgcgctcgc
     1801 agaaggcggg ccgccgccgg cggggagtat cggcggaatc aaccggacgg ggaaggacgg
     1861 ctactacgga ccctgcccgc cgcacggcga tgcgccgcat cactatgtcg tgcaagctta
     1921 cgcgctggat ctcgccgcca atgccctgcc cgccgcgctc gaccgcaacg ctttgatcgc
     1981 agcgatgaag ggtcacgtgc tggcgtcgtc gagtacggtg gtgcggtacg cgcgttgact
     2041 atcgaccaag gggcgatccg tgatcaagta gaccatcaac gcatcgcggc ttatgtcttc
     2101 aggcttcatc cgcggccgcg agtcccgccc tcgtctgcac acgccatgca cgtgaaccgc
     2161 cgagaaagtc gacccagccg agcgccgcgc ccgtgtggcg cagcaactgg aacgtgaacg
     2221 gctcgacaat cgccgtcacg acggccatcc acaggcttga gccagccgtg tctcccgtcc
     2281 agcgccggta cagatgcacg gtccacaaat ggaacgcgaa atcgatcaga atcttcgcgc
     2341 cgatcacact gaaaatcggc agcacaatag cgccgcgccg atccacgata aaccccagca
     2401 acagcgcaaa cgccgtcagg ccgtagatag gctgcacggt atcgaacgct ttcacgggca
     2461 gcatcagcaa accgagcgtg ccgtaacgcc ggttgccggt catatcgcga ttccagtatt
     2521 gcgtctgcaa aaagcccgcg aaccagcgcc gccgttgccg catgaagccg ctaaacgaac
     2581 tcggcgcgtc cgtgatggcg tgggcgttgc ccaccacccg cacatcccag tccagaccgt
     2641 gatcgacgga atgacggcgc aaccggtgaa tcagttcgta atcctccacc aggcattgcg
     2701 gatcgaaacc gccgacggcc agcaacgcat cgcggcgaaa tgaggcgaac gcgcccgaga
     2761 tcagcagcag gctgtcggcg cgcatccacg cgaaccgcgc gatgaaattg cggatgtatt
     2821 cgtaagtctg gaaccactgg aaaaagcgcc cgctcatcgt tttgctgcac accgggacca
     2881 ggatgcccgc cgccgctacc aggttcgatt gcaatgcaaa cgcactgcgc atggccagaa
     2941 cggcgtcgtc ggcgagacgc gtatcggcat cgactgtcat gacgatcggc gtgctgatct
     3001 tttcgatcgc cgcgttcagc gcacgggcct tgccgccatg cgccacgcgc agccagaaga
     3061 gcgtcggatg cagagtggac gcggcgctca actcgccctc cggcggcgcg acaaggccaa
     3121 aacgctgcgt gagaagctcg gcggtagcgt ccgaagaacc gtcgtcagca atcacgattt
     3181 gggccgcgcc gtgcgtttgc ccgagcaatt tcgcgagcgt gttcggcagc acggccgctt
     3241 cgttatgcga cgccacgatc acgccaagcg gcggcaaacc gcgttcgcgt tgcttgcgat
     3301 caagcggctt ctgcggcctg acgagcgtcc acgacttcgc cgcgacgaac atcagcagca
     3361 aggtgtcgta gaccacgtag gcaatgcctg tcgaccatgc caccacgccg tgcaggaaaa
     3421 acgcgcgggc gaacagcaca agccacagca cgaccacgcc gccgtgaatc agcacgctcg
     3481 cgaccgtgat cgggcgcggc gccaaacgcg gcgacgcctg cgcgagcgcc tcttccaggg
     3541 ttcgcttcac aagtggatcc ggttggggtg gagtcttttt tggtcgcgtg ggtacgacgt
     3601 gaagtttcac atccattacg gcaccatccg cgagatcacc gacttcttgt cgatccactg
     3661 atgcttgagc gcgccaccca cgtgcatcgc taaaagcgcg tacagcgcgt aaccgagcca
     3721 cgtatgcaat gcgccgaaac gatcgtgcag cgactccttc agcgccggat cgaggttcat
     3781 cacaaaaccc acacgcggcc acggaatcac gccgaacaga tgcatcggat gcgtcgcggc
     3841 gtctttccat gccgagtcgt gcagccagcc ggataacggc aagccgatca tcagcagata
     3901 cagcacaaag tgtgcgatgt gagcggccat gtgctcccat ttgggaaacg ccttcggcaa
     3961 cggcggcggc cgatgcgaaa cccgccacaa gatacgcagc agcaccagac cgagaaccgt
     4021 gatgccgatg gacttatggg tatcgacgac cggacggatc catgaatcag gaaacgattc
     4081 cgccgtcagt cccagcaaga cgttgcagat aatcagcaac gcgatgagcc agtgcaggac
     4141 catcgcagtg cgcgtatagc gcagcggcat ctcggtctga ggcggttctg acaccacgtt
     4201 ggcagcttgc gtggacattg aaactccgtg cgaaaaatga ggatcggctg gataggtctg
     4261 cgacctgtcg cgccggattg gtctattcaa gcgcgaatgt ttgcagacga cacgatccaa
     4321 acgcaccgtg acgccggttt attccatcgg ttctgcggga aaacgctaac ttgcgtttgt
     4381 agaggaattg tcaagtgagg tggtgagagg tatcggggga ttcagcgccc ttcggcgaca
     4441 caaacgccag caccgcttga gcgaattgca acgcagccgg attcgccgcg tcgcgacgag
     4501 tgacaaggtg gagcggcgct gagaacgctt cgtcgccggc aactgcgcaa tacaccacgg
     4561 tttcctgctg gtatcgctgc atggatgccg gtacgagcgt gatgccgccg cccgccgcca
     4621 ccagattgat ggcgctcagc atgcgcggca cttcgctcac cacctccggc ttgaatcctg
     4681 cctcctcgca cgcgcggatg aagtcagcgt acatacccgg tgcgcccggc cgccgcacga
     4741 agataaacga ctctccgaca agcgccttca gcggcacccg cggctgtttg cccggacgca
     4801 cgcctttcac cggcagcagc ttgtgcccga ccggcagcac gagcaccatg cgctcctcgt
     4861 gcagcaggtc gaagcgcaac tccgccggca tctcgatcgg cttgcgcaag atgcccgagt
     4921 cgacacgtcc attcatcatc cgttcgatga tgtcagccgc gttgatctcg ttcaggtcga
     4981 tggcaatacc aggatgcgtc gcgcgaaaac ggcggatcgc ggcggtggca agcgggtgga
     5041 atgccgccga actcgtcagc gcaatccgca cggagccgag ttggccgttc gccacccgcc
     5101 gggcattgac cacgccttga tcgagcgcgt cgagcacggc gcgggcatcg acgacaaaag
     5161 ccgcgcccgc cgatgtcagc tccacccctc gcggcaagcg cgtgaaaagc gcaaaaccca
     5221 gttcgcgctc gagctcctga atctgctgcg acaacggcgg ctgctgaata tgcagccgcg
     5281 ccgccgcccg cgtgaactgg cgctcttcgg cgacggcaag gaagtaccgc aggtgacgca
     5341 gttccatcta aaggcctcat atacgtgatg aatatggcat ggccatccga tatgtatttt
     5401 tcatttgcca ctgcgtttcg tactcttcga ttcaaataca cataaatgca cgaaccggag
     5461 acatatggaa accactttcc cacccaattc ggccgagcat gcgccagctt cattggcgtc
     5521 gctcagcaag gcgcagattc gccgcgccgt gctggcttcg gtgatcggca atgggctgga
     5581 atggttcgat ttcctgatct acggctactt cgcgaagatc atcgcgcatg tgtttttccc
     5641 ggttggcaac acggcgctct caacgacgct gacgctcggc acgtttgcgg ttggattcat
     5701 cgtgcggccg ctcggcggga ttgccatcgg cgcttatgcg gatcgcgtgg ggcggcgcaa
     5761 gacgctttcc atgctcatcc tgttgatggc cgccagcacc ttgttgatgg gcctgacgcc
     5821 cggctattcg actatcggga tcgccgctcc tttgctggtg ctgctcgcac gcgtcatgca
     5881 gggtttatcg gttggcggcg agttcgccac ggccgccgcg atgctcacgg aatatgcgcc
     5941 gcgcggcaag aagatgttct tcggcagctt ccagatgaca tcgcaggcgg tggcgctcgt
     6001 gctttcgtcg gcgtttggtt atttcctgac atcgaatctg gaccgcgcgt cgcttgagtc
     6061 gtggggctgg cgcgtgcctt tcctggtcgg cgcgctggtc gggccggtgg ggttttatat
     6121 ccgccacaag gtcgcggaat cgcccgagtt cgtcgagttg cgtgcacggc tcggccacgc
     6181 gccgcggcag accattcgcg gttttttccg gcagcgtggc gacgcgtcgt tgtgcgctat
     6241 ggg
//
LOCUS       DATASH010000037        20414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_400391,
            whole genome shotgun sequence.
ACCESSION   DATASH010000037 DATASH010000000
VERSION     DATASH010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 20414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20414
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400391"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VHV99_01240"
     CDS             <1..259
                     /locus_tag="VHV99_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634114.1"
                     /translation="MRLYVFDKDATPRTIACTGGCMSNWPRAMANPWDKPSADWTMIP
                     AADGKPQWAYKCHPLYRYAVDKQANDLKGDSFKDMWQTAKP"
     gene            complement(374..640)
                     /locus_tag="VHV99_01245"
     CDS             complement(374..640)
                     /locus_tag="VHV99_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634115.1"
                     /translation="MKALFHACAAAATAGVLLATVSVAFAQNSPGVPGGILQDQFRLA
                     EHPQMPFAASAPSDKYQSDKKSAMRKRGDNGDPNGCNLQCPKDE"
     gene            complement(750..1829)
                     /locus_tag="VHV99_01250"
     CDS             complement(750..1829)
                     /locus_tag="VHV99_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012400139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX3634116.1"
                     /translation="MIRIECQAQLVVKGSDGREASLSDVVPLLALVDQSGSIAQAATL
                     KGLSYRHAWGLLRSIEERLGGPLIAKERGRGSVLSELGQAVLRAQRLCGERLDGNMQA
                     LASEVASELNRWLAPPADDVRIYASHGYAVAALVTALVANDLPVDIKYRDSADAVSAL
                     ARGECDLAGFHLPLGEFRAACADTYRRWLDPQRHVLVYLTRRKQGLFLGKGNPKGIGG
                     LSDLARDDIRFVNRQPGSGTRMLLDLALRKVGVDPDRVNGYASAELTHSAIAAFVASG
                     MADVGFGVEPAAHHFGLDFIPIVDEDYYFACDRARLERAPLATVIGLLRGTAFRRSVE
                     QLEGYDPRDCGKLLDLDEGLGPTAV"
     gene            1962..2447
                     /locus_tag="VHV99_01255"
     CDS             1962..2447
                     /locus_tag="VHV99_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEX3634117.1"
                     /translation="MDDSMAIAPDQLVQRHAQPGMTLLAVLHAIQDELGYVPPATVAP
                     LARAMNLSRAEVHGVITYYHHFRTEPAAPVTVQLCRAEACRSMGTEALAQHIEAHTGC
                     RFDAEHALGATVELESVYCLGQCALSPAMMVNGTLHAKVTPQKFDALFAAASKCVEVT
                     A"
     gene            2444..4012
                     /locus_tag="VHV99_01260"
     CDS             2444..4012
                     /locus_tag="VHV99_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018435634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEX3634118.1"
                     /translation="MTRIYVPRDSSALAVGADELARAIEAEAARRGIEIELVRNGSRG
                     LLWLEPLVEVATAEGRVGYANVEETDVAALFDAGFVEGGEHARRVGVVDEIPYLKKQQ
                     RLTFARIGITDPLSVDDYVAHGGLEGLKQALKTDGDAACEALIESGLRGRGGAAFPAG
                     IKWRTVRGAKAAQKYIVCNADEGDSGTFSDRLVMESDPYVLIEGMIIAGVVTGATVGY
                     IYVRSEYPHSIATLEAAIAKARAAGWLGDSVLGSAHRFDLFVAKGAGAYVCGEETALL
                     ESLEGKRGIVRAKPPVPALVGLFGQPTVINNVITLATVPIIFARGAAFYKDFGMGRSR
                     GTLPFQLAGNVKQGGLVELAFGVTLRELLYDYGGGTASGRAARAVQVGGPLGTYLPES
                     QWDIPMDYEAYAAVGAVVGHGGIVVHDDTSNLAELAQYAMHFCALESCGKCTPCRIGS
                     TRGVEVIGRIRNGDTSTRQVQLLRDLCDTMVSGSLCAMGGMTPFPVLSALDHFPEDFG
                     LDNVHDHAPKAAAA"
     gene            4027..7005
                     /gene="fdhF"
                     /locus_tag="VHV99_01265"
     CDS             4027..7005
                     /gene="fdhF"
                     /locus_tag="VHV99_01265"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HEX3634119.1"
                     /translation="MSDVLNSRSGAPKEVHLGGGCGSGNCACKSQAQAQQRAPHSFFD
                     DTDYGTPARHADVDVTLEIDGQAVTVPAGTSVMRAAAEAGVNVPKLCATDSLEPFGSC
                     RLCLVEIEGKRGYPASCTTPVEAGMKVRTQTDRLQSLRRNVMELYISDHPLDCLTCPA
                     NGDCELQDMAGVTGLRDVRYGFDGANHLKDKKDESNPYFTYDPSKCIVCNRCVRACEE
                     TQGTFALTIAGRGFESRVAASENKPFMESECVSCGACVAACPTATLQEKTVIMLGQAE
                     HSVVTTCAYCGVGCSFKAEMKGNQVVRMVPHKNGQANEGHACVKGRFAWGYATHKDRI
                     TKPMIRAKITDPWREVSWEEALSYAASEFRRIQAKHGRDSIGGITSSRCTNEETYLVQ
                     KLVRAAFGNNNVDTCARVCHSPTGYGLKTTLGESAGTQTFASVDKADVIMVIGANPTD
                     GHPVFGSRMKRRIREGAKLIVVDPRRIDIVDTPHVKASHHLQLRPGTNVAVVNALAHV
                     IVTEGLLNEAFIAERCETRAFEQWRAFVALPENAPEATEELTGVPAASIREAARIYAT
                     GGNAAIYYGLGVTEHAQGSTTVMGIANLAMATGNIGREGVGVNPLRGQNNVQGSCDMG
                     SFPHELPGYRHISDSITRALFEEAWNVTLQPEPGLRIPNMFDAAVHGTFMGLYCQGED
                     IVQSDPNTHHVSAALSSMECIVVQDIFLNETAKYAHVLLPGSSFLEKDGTFTNAERRI
                     SRVRKVMPPVPGYADWEVTVMLSRALGYEMDYTHPSQIMDEIARLTPTFHGVSYKKLE
                     ELGSIQWPCNENAPDGTPTMHIDAFVRGKGKFVITKFIATPEKVTRKFPLLLTTGRIL
                     SQYNVGAQTRRTENSRWHDEDRLELHPHDAEERGIKNDDWVGIESRAGQTVLRAKVTE
                     RMQPGVVYTTFHFPESGANVITTDSSDWATNCPEYKVTAVQVMPVEQPSQWQQDYSRF
                     NTEQLDLLKQRELANATSGK"
     gene            7014..7262
                     /locus_tag="VHV99_01270"
     CDS             7014..7262
                     /locus_tag="VHV99_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit delta"
                     /protein_id="HEX3634120.1"
                     /translation="MDNQSLIDMANRIGDFFDSMPDREEALAGIADHIRRFWEPRMRR
                     ALLAALDEPDANGIEMSGIVREALVKHRDALTPAAAIA"
     gene            complement(7379..9103)
                     /locus_tag="VHV99_01275"
                     /pseudo
     CDS             complement(7379..9103)
                     /locus_tag="VHV99_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433996.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
     gene            complement(9172..10266)
                     /locus_tag="VHV99_01280"
     CDS             complement(9172..10266)
                     /locus_tag="VHV99_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX3634121.1"
                     /translation="MKKNSGRGHGSHASHEHSGHSHGNHAGQDHSGQSHGSHASHNHI
                     GHSHGSDAGQNHSGHNHGSHAGHHHAHAPVSGHGRAFALAVALNVAIVVVQAIYGVIA
                     HSTALLADAGHNLSDVLGLLLAWGAAWLATRRPSARYTFGYGSSSILASLANAALLLF
                     ACGVIVAEAIGRLVNPAPVAGLAVFIVATVGVVVNGVSAWLFMRGQKEDLNIRGAFLH
                     MAADAGISAAVAISGLVILYTSWTWLDPVMSLLVVAVVVYGTWGLLRDSVRMALDAVP
                     PGVDLQPIRDYLAGQPGVTDVHDLHVWALSTTGNALSAHLVMPAGHPGDESIDSIVIT
                     LRERFSMQHATLQVDLGTTEHRCAMDHPHD"
     gene            complement(10263..10610)
                     /locus_tag="VHV99_01285"
     CDS             complement(10263..10610)
                     /locus_tag="VHV99_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX3634122.1"
                     /translation="MVSTILPASFEDRPGETIVPLADLFRLLGDPTRLRIVLACVDER
                     RAVGAIAQGLGLSPSLVSHHLRLLRAARIVRAERQGKQVFYLAADRHISAMLAGMLEH
                     VAEPAGEFPLDPS"
     gene            10750..12597
                     /locus_tag="VHV99_01290"
     CDS             10750..12597
                     /locus_tag="VHV99_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3634123.1"
                     /translation="MQQVFDRAFAAHRDGRLEDAERDYRATLDGNPVHVDALHLLGVL
                     RHQQGQHAEAAELVRRAVNLRPEDAALQLNLGNALKALGQIDDAIEQFRNALTLAPSF
                     PMAHYNLGNAYASVGRHEDAADAFEKSLRLQPNDASSHNNLGNALHALGRHAEAVASF
                     QRTLELRPGHAGALNNMGMSLNALNRADEAIPSFQAALAAEPRFVAAHFNLANTLDAT
                     GRHEEAVASFRTALALQPNLPPTIFGLGNALAALGCHAEALPYLERAVGLDPQFALAW
                     LSLGTAHQALGAHAAAVRAFDQALRLRPDLASAHMNRALAWLALRDFARGLPEYEWRL
                     QTTAQPVIQTLPRWHGEPIEQRTLLIHAEQGFGDTLQFVRFVPLAAQRAARVVLEVQP
                     QLLPLLAPAAQAWRVTLIAQGAPRPAADLQCPLLSVPLALGTTCDTIPARTPYLGAPP
                     AYGRKWRGSLGGQAKRKIGIAWSGRLQKNETRSMPLAALAPLFALEGIDWIVLQPELS
                     AEERAALDAHPRAASIHRFDQRIGDFADTAAIIERLDAVVSIDTSIAHLAGALRKPLW
                     LMLPFAADWRWFVGETRSPWYPGATLLRQPQPGAWGDVVEAVANELRGG"
     gene            complement(12786..14087)
                     /locus_tag="VHV99_01295"
     CDS             complement(12786..14087)
                     /locus_tag="VHV99_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3634124.1"
                     /translation="MSTASHATSSSEESKVKTVFRVVSGNFLEMYDFMVYGYYASAIA
                     KTYFPSGNEFASLMLSLSVFGAGFLMRPLGAIVLGAYIDHHGRRKGLILTLGLMALGT
                     LTVAAIPGYATIGVLAPALVLFGRLLQGFSAGVELGGVSVYLSEIATKGNKGFYCAWQ
                     SGSQQVAVVFAALIGVFLNKWLPADQMTAWGWRVPFLIGCLIVPFLFLIRRSLQETEE
                     FKARKHHPSMGEIMKTMAANWGIVLGGMGMVIMTTVSFYMITAYTPTFGKEVLKLSSI
                     DTLVVTVCIGLSNLVWLPLAGALSDRIGRRPVLLAFTILTIVTAYPALQWLVAEPSFV
                     RLLAVELWLSFLYGSYNGAMVVALTEVMPVEVRTAGFSLAYSLATTIGGFTPAISTLL
                     IHTTGNKAAPGLFLGVAAICGLIATLVLYRTPESRDQYRTA"
     gene            14401..14925
                     /locus_tag="VHV99_01300"
     CDS             14401..14925
                     /locus_tag="VHV99_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3634125.1"
                     /translation="MQTSHALSLRPATLADAALIASIHSTSWQATYRGLLPDAFLDGD
                     VMQERATYWEARLRAPGGERRIVLIAELAGEPIGFVCVERQPESAWGVLLDNLHALPG
                     HQGLGAGKLLMRAAEDWARTQGETQLYLYVLEGNTPAIGFYEKQGWQFVGAEPDHMGG
                     VDITALRYVYRLDR"
     gene            15484..16347
                     /locus_tag="VHV99_01305"
     CDS             15484..16347
                     /locus_tag="VHV99_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634126.1"
                     /translation="MVDGSVDASVDGSLRTVAEAAPFSPVSAPGVPASPHGGSRHWGA
                     LAGGACALGGAAILAWIAVGHLTQRDTIGEVKTAGKFPVRQEAQLANHHSPDAAATPY
                     ATDNDRAAAHEVLPNALATAPLRDLTTSPASEIVTPVKAAPARATTGKNGSRRRDRQR
                     ESAAIQHERRGRNSRTAITENLSPQLPSQLPHASPMNYEGSQIAVSANAHRTSPKPSS
                     AGPYSPLAPSQLGTDEYASMTLSAGTHLRNIAPPSRPAASVNSPGISGTEWINHMSQR
                     RVTEDPDQFAN"
     gene            complement(16469..17185)
                     /locus_tag="VHV99_01310"
     CDS             complement(16469..17185)
                     /locus_tag="VHV99_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2968 domain-containing protein"
                     /protein_id="HEX3634127.1"
                     /translation="MKYPLIVRRAMLLAASCALLQHGGFVLAAQSDEAAPLVGTRPAM
                     NTLTPQPVAAALRNAASSTPASANDAQGNVAELMQMIHDAKLNELRTTYNGSYGASLF
                     FYPQEMTYYVALFQDKHFWRVIKSQDTTRAEAVYAGFVQQTAQLADVEIRRTQLQAQK
                     AYIEDIIALSEDRAKRLQADLDVARAQQAKVNDYQRQTQGEAVALHAEKEKAQAQLRQ
                     VQGEVMQLQRQTEFGLPTQK"
     gene            complement(17799..19238)
                     /locus_tag="VHV99_01315"
     CDS             complement(17799..19238)
                     /locus_tag="VHV99_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434004.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEX3634128.1"
                     /translation="MSENKPENAGKPGGPASPSATPGTTATPTVSGTPRSTALPGDSS
                     ANVPAASSTSPAAEAAAVRSSTDDPQSASTVTPTDAQMARQRDAAEARSAAAASTAQQ
                     EAAVHERAAAKAGHASRVQAPRPATLESSEPLAGELSVSVDEATVTEKAQEAATRPAG
                     SPPPGFGAAPDFTATNPPPPNALPPSPPRYLKQNDSAWTVFGRIIAARARQLFDRAGQ
                     RITQRTLRIGVSARIFHPEPGAKGLRGKTLQYLEESIAHWVMSRDVLVFMIPTVGHQG
                     MLHPSNIRLRDYAKHLDGLLLQGGADVSPQSYAEQATSPEWPGDRVRDMYELELLHEF
                     IESGKPVLGVCRGCQLINVAFGGSLYQDIATDVPTAGTHVNENYDQHRHGIHFPDGST
                     LANMFPGRRDAIVNSIHHQAVKTLGRDLNIEAVSASDGIIEAVRYRRAPFVMGVQWHP
                     EFHRAGGPELLDCTPLLDTFLRVARETRF"
     gene            complement(19382..>20414)
                     /locus_tag="VHV99_01320"
     CDS             complement(19382..>20414)
                     /locus_tag="VHV99_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX3634129.1"
                     /translation="SIALGAFLIGAIMAESRHLHRIEHLIGPLRDAFSAIFFVTIGLM
                     LNPAVLVDYAWPIAVITVAVIVGKIVSCGLGTFLAGKDGRTAMRVGMTVSQIGEFSFI
                     IASLGLTLKVTSAFLYPIAVAVSALTTLFTPYLIRAADPLTQRLGQAMPRTLANGFGM
                     YGQWLRSLSTETGEPTLFSMTRRIILQIAVNLALVAAIFLIVSYSAPYSSTWLAHWIS
                     SEPMQRVVLWSIALVLSMPFLVAVYRKTKSLALLLAEVSVQPATAGRFTRAIRYAISD
                     LVPVVSMVGVFLLVAALSGSILPPTGLLTVVLICAAVLLALVWRWCVKIHAAMQIALR
                     ETFEEQPDP"
BASE COUNT         4001 a         6621 c         6334 g         3458 t
ORIGIN      
        1 catgaggctc tatgtcttcg acaaagacgc cacgccccgc acaattgcct gcaccggtgg
       61 ctgcatgagt aactggccgc gggcaatggc gaacccgtgg gacaaaccat cagccgactg
      121 gacgatgatc cccgctgcag acggcaagcc gcaatgggcg tacaagtgcc atccgctata
      181 ccgctacgcg gtcgacaagc aggcaaacga tctgaaaggg gatagcttca aggacatgtg
      241 gcagacggcg aagccataac cgggagtcgc tgaccaacgg gccaggactg gaagcctaaa
      301 aacgctgaaa ctaaaaacgc cggcagcatg ccggcgttct cacagactca cacgagagcg
      361 ccgaccttcg cgcttactcg tctttaggac actgcaagtt acatccattc ggatcgccgt
      421 tatcaccgcg cttgcgcatg gccgactttt tgtcggactg gtatttgtcc gaaggtgccg
      481 acgccgcaaa cggcatctgc ggatgttcgg caagacggaa ctgatcttgc agaataccac
      541 ccggcacacc cggagaattc tgtgcaaaag cgaccgaaac ggtggcgagg agcaccccgg
      601 ctgtcgctgc cgcagcacat gcgtggaaaa gagctttcat atcgagtcag cgcggtacag
      661 cgcgcggttg tggcgtaaac ggaaggttag agattatccc aaacagacgc cccacgcagc
      721 cgcgacggca ccattttgtt tacagtcgct tacaccgcag tcgggccaag cccctcatcg
      781 agatcgagca gcttgccgca gtcgcgcgga tcgtaacctt ctagctgctc gacgctccgc
      841 cgaaacgccg tgccgcgcag caagccgatt accgttgcga gcggcgcgcg ctccagccgg
      901 gcgcggtcgc aagcgaagta atagtcttcg tcgacgatcg gaatgaagtc gagcccgaag
      961 tggtgcgccg ccggctcgac gccgaaaccc acatccgcca tgccgctcgc cacgaaggcc
     1021 gcgatcgccg aatgcgtgag ttccgccgac gcataaccgt taaccctgtc cggatcgacc
     1081 ccgacttttc gtagcgcgag atcgagcagc atgcgcgtac cggagccagg ctgacgattg
     1141 acgaagcgga tgtcgtcgcg cgcgaggtcg ctcaggccgc cgattccctt gggattgccc
     1201 ttgccgagaa agaggccctg cttgcgccgc gtgagataca cgagcacgtg acgctgcgga
     1261 tcgagccagc gccgatacgt gtcagcacag gcggcgcgga attcccccag tggtaagtgg
     1321 aagccggcca gatcgcattc gccgcgggcg agcgcgctga ccgcatccgc gctgtcacgg
     1381 tatttgatat cgaccggcag atcgttcgcg accagcgccg tcaccagtgc cgccaccgcg
     1441 taaccgtgcg acgcgtagat ccgcacgtcg tcggcgggtg gcgcgagcca gcggttcagt
     1501 tcgctggcga cttcgctcgc gagcgcctgc atattgccgt cgagccgctc gccgcacaga
     1561 cgctgtgcac gcaacacggc ctgcccgagt tcggacagca ccgagccgcg cccccgctcc
     1621 ttcgcgatca gcggaccacc caaacgctcc tcgatgctgc gcaacaaacc ccacgcgtga
     1681 cggtaggaca aacccttaag cgtcgccgcc tgcgcgatgc tgccggattg gtccaccaac
     1741 gcgagcaacg gcaccacgtc cgacaggctg gcctcgcggc cgtcggagcc cttcacaacc
     1801 agctgcgcct gacactcgat tctgatcata tatgctcaac tatttcctat tgcgcgaggc
     1861 gtttcacccg cctattgttc cgctatccca tattgaatag agaaagcata agtgcacaca
     1921 ttatgaatca caagtgcata tatgacttga aggagcctca cttggacgat tccatggcca
     1981 tcgcgccgga ccagctcgtg cagcgtcacg cgcagccggg catgacgttg ttagcggtat
     2041 tgcacgcgat tcaggacgaa ctcggctacg tcccgcccgc cacggttgcg cctctcgcgc
     2101 gtgccatgaa cctgtcgcgc gcggaagtac acggcgtcat cacctactac catcacttcc
     2161 gcacggagcc cgcagcaccc gtcacggtgc aactatgccg cgccgaagcg tgccggagca
     2221 tgggcaccga ggcgctcgcg cagcatatcg aggcgcacac cggctgccgt ttcgatgctg
     2281 aacacgcgct aggggcgaca gtcgagctgg aatcggtgta ttgcctcggt caatgcgcgc
     2341 tgtcgccggc gatgatggtc aacggcaccc tgcacgccaa ggtgacgccg caaaaattcg
     2401 acgcgctgtt cgccgccgcc agcaaatgcg tggaggtcac ggcatgacgc gcatctacgt
     2461 tccccgcgat tcgtcagccc tggcagtcgg cgccgacgaa ctggctcgag caatcgaagc
     2521 cgaagccgca cgccgcggca ttgagatcga actggtccgc aacggttcac gcggtttgct
     2581 gtggcttgaa ccgctcgtcg aagtcgcgac ggccgaaggc cgtgtcggct atgccaacgt
     2641 cgaggaaacc gacgtcgcag cgctattcga cgccggcttc gtcgagggcg gcgagcacgc
     2701 ccgccgcgtc ggtgtggtcg atgaaattcc gtatctgaag aagcagcagc gcctgacctt
     2761 cgcgcgcatc ggcatcaccg atccgctttc cgtcgacgac tacgttgccc acggcggcct
     2821 cgaaggtctc aagcaagcct tgaaaaccga cggcgacgcc gcctgcgaag ccctgatcga
     2881 atcgggtctg cgcggccgcg gcggcgcagc cttccccgcc ggcatcaaat ggcgcacggt
     2941 gcgcggcgcc aaggcggcgc agaaatacat cgtctgcaat gcggacgaag gcgactccgg
     3001 gactttctcc gaccgcctgg taatggaaag cgacccgtac gtgctgatcg aagggatgat
     3061 cattgcgggc gtcgtgaccg gcgcgacggt cggctatatc tatgtacgca gcgaatatcc
     3121 gcactcgatc gcgacactcg aagccgcgat cgccaaagca cgcgccgccg gctggctcgg
     3181 cgacagcgtg ctgggctcgg cgcatcgctt cgacctgttc gtggcgaagg gtgcaggcgc
     3241 ttatgtctgc ggcgaggaaa ccgcgctgct cgaatcgctc gaaggcaaac gcggcatcgt
     3301 gcgcgcgaag ccgcccgtgc cggcgctagt cggcctgttc ggccagccga ctgtgatcaa
     3361 caacgtcatc acactcgcca ccgtgccgat catcttcgcg cgcggcgcgg ccttctataa
     3421 agactttggc atgggccgtt cgcgcggcac gctgccgttc cagctcgccg gcaatgtgaa
     3481 gcaaggcggc ctcgtcgagt tggcgttcgg cgtcacgctg cgcgaattgc tctatgacta
     3541 cggcggcggc acggcaagcg gccgcgcggc gcgcgcggtg caagtcggtg ggccgctcgg
     3601 cacgtatctg ccggaaagcc agtgggacat ccccatggac tacgaggcct acgcggcagt
     3661 cggcgcggtg gtcggccacg gcggcatcgt cgtgcatgac gacacctcga atctcgccga
     3721 actcgcgcaa tacgcaatgc acttctgcgc gctggaatca tgcggcaagt gcacgccgtg
     3781 ccggatcggc tcgacgcgcg gcgtcgaagt gatcggacgg atccgcaatg gcgatacgtc
     3841 cacgcggcag gtgcagttgc tgcgcgatct gtgcgacacg atggtgtccg gttcgctctg
     3901 cgcgatgggc ggcatgacgc cgttcccggt gctgtccgcg ctcgaccatt tccccgaaga
     3961 cttcggtctc gacaacgttc acgatcacgc gcccaaagcg gctgcggcct gatccaggag
     4021 cctcatatgt ccgatgtgct caactcccgc tccggcgccc cgaaggaagt ccacctgggg
     4081 ggcggctgcg gctccggcaa ctgcgcgtgc aaatcgcaag cacaagcgca acaacgtgca
     4141 ccgcattcgt tctttgacga caccgattac ggcacaccgg cgcgtcatgc cgacgtcgac
     4201 gtcacgctgg aaatcgacgg tcaggcggtg acggtgccgg caggcacttc agtcatgcgt
     4261 gccgccgccg aagccggcgt caatgtgccg aagctctgcg cgaccgattc gctcgaaccg
     4321 ttcggctcgt gccgtttgtg cctggtcgag atcgaaggca agcgcggcta ccctgcttcg
     4381 tgcacgacac ccgtcgaagc cggcatgaag gtccgcacgc aaaccgaccg cctccaatca
     4441 ctgcgccgta acgtgatgga gctgtacatc tccgatcacc cgctcgactg cctgacctgc
     4501 cccgccaacg gcgactgtga attgcaggac atggcaggcg tcaccggctt gcgcgacgta
     4561 cgctacggat tcgacggtgc gaaccacctg aaggacaaga aggacgaatc gaatccgtac
     4621 ttcacgtacg atccgtcgaa gtgcatcgtc tgcaaccgct gcgtgcgcgc atgcgaggaa
     4681 acgcaaggca cgttcgcgct gaccatcgcc ggacgcggtt tcgaatcgcg cgtcgcggcg
     4741 agtgaaaaca agccgttcat ggaatcggag tgcgtgtcgt gcggggcatg cgtcgccgcg
     4801 tgtccgaccg cgacgttgca ggaaaagacc gtcatcatgc tcggccaggc cgagcattca
     4861 gtcgtcacca cgtgcgcgta ttgcggcgtc ggctgctcgt tcaaggcgga gatgaagggc
     4921 aaccaggtcg tgcggatggt gccgcacaag aacggccagg ccaacgaagg ccacgcgtgc
     4981 gtgaagggcc gcttcgcatg gggctacgcg acgcataagg accgcatcac caagccgatg
     5041 attcgcgcga agatcaccga tccgtggcgc gaagtcagct gggaagaagc gctcagctac
     5101 gcggcatcgg aattccgccg cattcaggcg aagcatggcc gcgattcgat cggcggcatt
     5161 acgtcatcgc gctgcacgaa cgaagaaacg tatctcgtac agaagctcgt gcgcgccgcg
     5221 tttggcaaca acaacgtcga cacatgcgcg cgtgtgtgcc actcgccgac cggctacggc
     5281 ctgaagacca cgcttggcga atccgcgggc acgcagacgt tcgcctcggt cgataaagcc
     5341 gacgtgatca tggtgatcgg cgcgaatccg accgacggcc acccggtgtt cggctcacgc
     5401 atgaagcgcc gcatccgtga aggcgcgaag ctgatcgtcg tcgacccgcg ccgcatcgat
     5461 atcgtcgata cgccgcacgt gaaggcctcg catcacctgc aattgcgtcc gggcaccaac
     5521 gtcgcggtgg tcaatgcgct cgcgcatgtg atcgtgaccg aagggttgct gaacgaagcg
     5581 ttcatcgccg agcgttgcga aacgcgcgcg ttcgagcaat ggcgtgcatt cgtcgccctg
     5641 cccgagaatg cgccggaagc gactgaggaa ctcacgggcg tgcccgccgc atccatccgt
     5701 gaagccgccc gcatttacgc caccggtggc aacgccgcga tctactacgg ccttggcgtc
     5761 actgaacacg cacaaggctc gacgacggtg atgggcatcg cgaatctggc gatggcgacc
     5821 ggcaatattg gccgcgaagg tgtcggcgtg aatccgctgc gcggccagaa caacgtgcag
     5881 ggttcgtgcg acatgggctc gttcccgcac gaattgccgg gctatcgaca tatcagcgat
     5941 tcgatcacgc gcgcgttgtt cgaagaagcg tggaacgtta cgctgcaacc cgaaccgggt
     6001 ctgcgcattc cgaacatgtt cgatgccgcg gtgcacggca ccttcatggg cctgtactgc
     6061 cagggcgaag acatcgtgca gtccgatccg aacacgcatc acgtgtcggc ggcgttgtcg
     6121 tcgatggaat gtatcgtcgt acaggatatt ttcctcaacg agaccgcgaa gtacgcgcac
     6181 gtgctgctgc ccggttcgtc gttcctcgaa aaggacggca ccttcaccaa cgcggaacgc
     6241 cgcatctcgc gcgtgcgcaa ggtgatgccg ccggtgccgg gttacgccga ctgggaagtc
     6301 acggtgatgc tctcgcgcgc gctcggctat gaaatggact acacgcatcc gtcgcagatc
     6361 atggacgaga tcgcgcgtct cacgccgacc ttccacggtg tgtcgtacaa gaagcttgaa
     6421 gaactcggca gcatccagtg gccgtgcaac gagaacgcgc ccgacggtac gccgaccatg
     6481 cacatcgatg ccttcgtgcg cggcaagggc aagttcgtga ttacgaagtt catcgccacg
     6541 ccggaaaagg tcacgcgcaa gttcccgctg ctgctcacta cgggtcgtat tctgtcgcag
     6601 tacaacgtgg gtgcacagac gcgccgcacc gagaattcgc gctggcacga cgaagaccgg
     6661 ctggagttgc atccgcacga tgcggaagaa cgcggcatca agaacgacga ttgggtcggt
     6721 atcgaatcgc gcgcgggaca aaccgtgttg cgcgcgaaag tgaccgagcg gatgcaaccg
     6781 ggcgtcgtct acacgacgtt ccacttcccc gaatcgggcg cgaacgtgat caccaccgat
     6841 agctcggact gggccaccaa ctgtcccgag tacaaggtga ccgcggtgca ggtgatgcca
     6901 gtcgaacagc cgtcgcaatg gcagcaggac tattcgcgct tcaacacgga gcagctcgat
     6961 ctgctgaagc agcgcgagtt ggccaatgca acttcgggta agtgaggcga gccatggaca
     7021 atcagagtct gatcgacatg gcgaaccgga tcggcgactt cttcgattcg atgccggatc
     7081 gcgaggaagc gctcgccggc atcgccgatc atatccgccg cttctgggaa ccgcgtatgc
     7141 gccgcgcttt actggcggcg ctcgatgaac cggatgcgaa cggcatcgag atgtcgggca
     7201 tcgtcaggga agcgctcgtc aagcatcgcg acgcgctgac gccggcagcg gcaatcgcgt
     7261 agcggcactg ttgcctggcg cctaccgtgt gaagcgaccc atgtcagggc tgcttcacac
     7321 ggtcgcgctc acgatctcac ccaggcaccc acctcgcagg caatctctcg gcccctcctc
     7381 acagcgccgc ctgctccggt atcaccccac ccgccgcgaa ccacgcttcg caatgccgca
     7441 gcaagccgtg cagatcggag ccggtgcgcc cacgcgcgca gatgtatccg tccggccgta
     7501 ccagatagaa cgacggccgc gtgcgtccat atgattcgga caacgccggg gcgccctcgc
     7561 cgctcgtatc ggtcacgcgc cagatgcgta ccgcgcccgg caacaatcgc tcgatttccg
     7621 ccgccagtcg ctcgagctcc gggtctggcg gcgctgcgtg tttcgcggcg ggatcgagcg
     7681 gcgtgccgtc gaccggtagc ggcgggacca gcaggaacaa cgaaaagaac gcgggatcgt
     7741 gcagatcgaa cacgcaaccg acgcccgggg cgcgcccgag cggcccgtcc accacatgca
     7801 ccagcgcatc cggcgcgcgc tcgccggcac gcggaccgcc gtccagcacg cgctcgagcg
     7861 tcagcggacc gcgccgatac tggatcgaca actcgctgat ggtcatccgc gccgcatcgc
     7921 gcagcggacc gagcgcggcc agcaccggca tcacacgctc gcgcagcagc ttcaacggcc
     7981 cgtggtcggc ctcggccata tgcgtgatga agccggtttg ccgtaacacg tcgcgctcga
     8041 tcggatgccg ttcgaggtgg tacgtatcga gcaggcggtc gggcgccgcg cctttcaata
     8101 cccgggcgag cttccagccg agattgaacg cttcctgaat gccggtgttc atcccttgcg
     8161 cgccggccgg actgtgtacg tgagccgcat cccccgcgag gaagatgcgg ccgacgcgca
     8221 gccgctccac catcctgctg ttcagatgga aatacgacga ccacgtcagg tcctacacgt
     8281 cgacgcgttc ccgcacgcgc cgggcgatca gcgccttgca ttcggcgagc gacggcggcg
     8341 aaacccgatt gagcggcggt tcgccgggca cggcgggtgt ggccggcgca gggacggtag
     8401 tcggttcgac cggatggtcc gcaatcagcc gatggcgccc gtgacccatc ggaaacaacg
     8461 cgacgaggcc gtcacccgaa gcgaagatat ggaattcgtc ttccggccaa tcggtttcgg
     8521 catgcaggtc ggcaagcagg aacgtctgct cgaaggtgct gcccacaaag ctcaagccga
     8581 gccgatgacg gatcgtgctg tgcgcaccgt cagcggcgat caggtacgaa ggccgcatgg
     8641 tttccgtatg accgtccgcg cgccgcaagg tcgcctgaat gctggcggaa ccttgcgaga
     8701 acatcgtcag ttcgacgccg cgctcgaccg tcacgccgag tgtcgcgaga tgctctgtca
     8761 gcagacgttc ggtcacggct tgatcgagaa acagcagata cggatagcgc gtgtgcagcg
     8821 gatcgaaatc gagacgcgcg agacgcatgc cattggaaaa aagattggcg atgcgggcgc
     8881 gatgaccgag ttcgagaaac ggctcgatca gccggtgctg ttcaaacagt tcgagcgtgc
     8941 gcgcctgaat gccgatggcc cgtgagtacg ggttcggttg caatgctttg tcgatcagac
     9001 gcacggggat gtgggcacgc gccaggctca ttgccgcggc gagcccggtc ggacccgccc
     9061 cgacgatcag caccggtgac gcatcgtgtg cggcagcgtc catgcggaac tccggaggca
     9121 acaggacgtt ctagtgtacg ccgccgataa tccgctcgac ggtgcgctgc atcaatcgtg
     9181 gggatgatcc atcgcacatc ggtgctcggt ggtgccgagg tcgacttgca gcgtcgcatg
     9241 ctgcatcgaa aaacgttcgc gcagggtgat gacgatgctg tcgatcgact cgtcgccggg
     9301 atggcctgcc ggcatcacca gatgcgcgct gagcgcattg ccggtagtcg acagcgccca
     9361 tacatgcagg tcgtgcacgt cggtgacgcc aggctgtccg gccagatagt cgcggatcgg
     9421 ctgcaggtcg acgccgggcg gcacggcatc gagcgccatg cgcaccgaat cgcgcagcag
     9481 gccccacgtc ccatacacca ccaccgcgac cacgagcagg ctcatcaccg ggtcgagcca
     9541 cgtccagctg gtgtagagaa tcacgagacc gctgattgca accgcggccg agatgccggc
     9601 gtccgcggcc atatgcagga aggcgccgcg aatgttcagg tcttccttct ggccgcgcat
     9661 gaaaagccac gccgagacgc cgttcaccac cacgccgacc gtcgcgacga tgaacaccgc
     9721 cagtcccgcg accggcgccg ggttcaccag ccgtccgatt gcctcggcga cgatcacgcc
     9781 gcacgcgaac aggagcagtg ccgcgttggc gagcgacgcg agaatcgacg aactgccgta
     9841 gccgaatgta tagcgggccg acggacggcg cgtggcgagc caggctgcgc cccaggcgag
     9901 cagcaagccg agcacgtcgg agagattgtg gccggcgtcg gcgagcagcg cggtggaatg
     9961 cgcgatcacg ccgtagatgg cttgaaccac cacgatcgcc acgttcagtg cgacggcgag
    10021 tgcgaaagcg cggccgtggc cggagacggg ggcgtgggcg tggtggtggc cggcgtggct
    10081 gccgtggttg tggccactgt ggttctggcc ggcgtcgctg ccgtggctgt ggccaatgtg
    10141 gttgtggctg gcgtgactgc cgtggctctg gccactgtgg tcctggccgg cgtggttgcc
    10201 gtggctgtgg ccgctgtgct cgtggctggc gtgactgccg tggccgcgcc cgctgttttt
    10261 cttcatgacg ggtccagagg aaattcgccg gccggctcag ccacatgctc gagcatgccg
    10321 gccagcatcg cgctgatgtg acggtcggcg gcaagataga acacctgctt gccctgccgt
    10381 tccgcgcgca cgattcgcgc cgcgcgcaac agccgcagat ggtggctgac cagcgacggc
    10441 gacaagccca gcccctgggc aatcgcgccg accgcgcgcc gctcgtcgac gcacgccagc
    10501 acgatgcgca agcgcgtcgg gtcgccgagc agccggaaca ggtcggccag cggcacgatc
    10561 gtctcccccg ggcggtcttc gaaactggct ggcaatatcg tggaaaccat tgcagaagac
    10621 gtcgtcggct aacatatgaa caggtattca catgttatag atatcgagac aacaggtcaa
    10681 atcggagaac ggcccggcga gtgtgggaaa atgcagcgtc cgcctcaccc gaaatgccat
    10741 ccctccgtca tgcaacaagt atttgaccgc gctttcgcgg cgcatcgtga cggccgcctc
    10801 gaagacgccg aacgcgacta ccgcgcgacg ctcgacggca atcccgtgca cgtcgatgca
    10861 ttgcacctgc tgggcgtcct gcgccaccag cagggccagc acgccgaagc cgccgagctc
    10921 gtgcggcgcg cggtgaacct gcgcccagaa gatgccgcgc tgcagttgaa cctcggcaat
    10981 gcgctgaagg cgctgggcca gatcgacgac gccatcgaac agttccgcaa tgcgctcacg
    11041 cttgcaccgt ccttcccgat ggcgcactac aacctgggca atgcgtacgc ctcggtgggc
    11101 cgtcacgaag acgccgccga cgccttcgag aaatcgttgc gcctgcaacc gaacgacgcc
    11161 tcgtcgcata acaatctcgg caacgcattg catgcgctcg gccgtcacgc ggaagctgtc
    11221 gcgtcgttcc agcgcacgct cgaattgcgt cccgggcatg ctggcgcgct gaacaacatg
    11281 ggcatgtcgc tgaacgcgct taaccgcgca gacgaggcga tcccgagctt tcaggcggcg
    11341 ctggcggccg aaccgcgttt cgtcgcagcg catttcaatc tcgccaatac gctcgacgcc
    11401 accggccgcc acgaagaagc cgtcgcctcg ttccggaccg cgctcgcttt gcagccgaac
    11461 ctgccgccca ccatcttcgg cctcggcaac gcgctcgcgg cgttgggatg ccatgcagaa
    11521 gcgctgccat atctcgaacg cgcggtcggc ctcgatccgc aattcgcgct cgcctggctg
    11581 agtctcggca cagcgcacca ggccttgggc gcgcacgccg cggctgtgcg ggcgttcgat
    11641 caagcgctgc gcttgcgccc cgacctcgcg tccgcgcaca tgaaccgcgc gcttgcatgg
    11701 ctcgcattgc gcgatttcgc gcgcgggctg ccggaatacg aatggcggct gcaaaccacc
    11761 gcgcaaccgg tcatccagac cttgccgcgt tggcacggcg agccgatcga acaacgtacg
    11821 ctcctgatcc acgccgagca gggtttcggc gatacgctgc aattcgtgcg ctttgtgcca
    11881 ctggccgcgc agcgtgcggc gcgcgtcgtg ctcgaagtgc agccgcaatt gctgccgctg
    11941 cttgcaccgg ccgcgcaagc ctggcgcgtg acgctgattg cccagggtgc accgcgtcca
    12001 gcggcggatc tgcaatgccc gctactcagc gtaccgctcg cgctcggcac aacctgcgac
    12061 acgatccccg cccgcacgcc ttacctcggc gcgccgccgg cttacggccg caaatggcgc
    12121 ggctcgctcg gcggtcaagc gaagcgcaag atcggcatcg cgtggtcagg acgtctccag
    12181 aaaaacgaaa cccgttcgat gccgctcgcg gcgctcgctc cgctgttcgc gctcgaaggg
    12241 atcgactgga tcgtgctgca acccgaactc agcgccgagg aacgcgcggc gctcgacgcg
    12301 catccgcgtg cggcgtcgat tcatcgcttc gatcaacgga tcggcgattt cgcggatacc
    12361 gcggcgatca tcgaacggct cgacgcggtt gtgtcgatcg acacctcgat cgcccacctg
    12421 gctggcgcgc tgagaaagcc gctgtggctg atgctgccgt tcgccgcgga ttggcgctgg
    12481 ttcgtcggcg agacgcgcag cccgtggtat ccgggcgcaa cgctgctgcg ccagccgcaa
    12541 ccgggcgcgt ggggcgacgt ggtggaggcg gtggcgaatg aattgcgcgg gggctgaccg
    12601 cggcggcgtt ggcgacatcc attgcagcaa cgtagcggac gccgaggcgg acgaattgcg
    12661 ccgcggctaa ccggtccggc gttaccagaa tccgtcacaa agcggtgtcc aaagaaaaaa
    12721 ccccgcgctc gtaagaacgc ggggttttag tttgtccgga tgaacggtga gctttaacct
    12781 tgcccttaag ccgttcgata ctgatcgcgc gactccggcg tgcgatacag caccagcgtc
    12841 gcgatcagcc cgcagatcgc cgcgacgccg aggaacaggc ccggtgcggc cttgttgccg
    12901 gtggtgtgaa tcagcagcgt cgagatggcc ggcgtgaagc cgccaatcgt cgtagcaagg
    12961 ctgtaggcca gcgagaaacc ggcagtccgc acttcgaccg gcatcacttc ggtcagtgca
    13021 accaccatcg cgccgttgta gctgccgtac aggaacgaca accacagttc gaccgccaga
    13081 aggcgaacga acgacggctc ggcgaccagc cattgcagcg ccggataggc ggtgacgatc
    13141 gtcaggatcg taaaggccag cagcacggga cggcggccga tccggtccga caacgcgccc
    13201 gcgagcggca gccagaccag gttcgacaga ccgatacaga cagtgaccac aagcgtatcg
    13261 atcgacgaca gcttcagtac ttccttgccg aaggtcggcg tataggccgt gatcatgtag
    13321 aacgacacgg tggtcatgat caccatgccc atgccaccca gcacgatgcc ccagttcgcg
    13381 gccatcgtct tcatgatctc gcccatgctc ggatgatgct tgcgcgcctt gaattcctcg
    13441 gtttcctgca acgaacggcg aatcaggaac aggaacggca cgatcaggca gccgatcagg
    13501 aacggcacac gccagcccca ggccgtcatc tggtcagccg gcaaccactt gttcaggaac
    13561 acgccgatca gcgcggcgaa caccacggca acctgctggc tgcccgactg ccacgcgcag
    13621 tagaagccct tgttgccctt ggtggcgatc tccgacagat acaccgacac gccgcccagt
    13681 tcgaccccgg cggagaagcc ttgcagcaat cggccgaaca gcacgagcgc cggggcaagg
    13741 acgccgatgg tcgcgtagcc cggaatagcg gcgaccgtca gggtgccgag cgccatcagc
    13801 ccgagtgtga ggatcaggcc tttgcgacgg ccgtgatggt cgatgtaggc gccgagcacg
    13861 attgcgccga gcggccgcat caggaagccc gcgccgaata ccgacagcga cagcatcagc
    13921 gaggcaaatt cgttgccgct cgggaaatag gtcttggcaa tcgcggacgc gtaatagccg
    13981 tagaccatga agtcgtacat ctcaagaaag ttgccgctga ccacgcggaa aactgtcttg
    14041 accttggatt cttcggacga ggaagtggcg tgtgacgcag tagacatgac attctctgat
    14101 aagcccgtcg ccacgatgac aggcggcgct caggcagttg aaacgatcca ccgcgcttgg
    14161 gcaggcggat gccacaggca atagggtaga catagaacgc gccgggggcg cggcattctc
    14221 atgccgaacg cattcgcacg gtaccagggt aaacgttgtg aaacagccga agcccgcaac
    14281 acgtaatgtt accgttcaag atactgccgc gcattccata cagttccatt acagacaacc
    14341 gcgctatacc cttgcgatga actttttgca gacaggcaat tcccgttgac caccatcccg
    14401 atgcaaacct cccacgcact ttcgctgcgc cccgcgacgc tcgcggatgc agcactgatc
    14461 gcctcgattc acagcaccag ttggcaggcc acctatcgcg gcctgttgcc cgatgcattc
    14521 ctcgacggcg acgtgatgca ggagcgcgct acctattggg aagcgcgcct gcgcgcaccg
    14581 ggcggcgagc gccggatcgt cctgatcgcc gagctcgcgg gcgagccgat cggtttcgtc
    14641 tgcgtcgaac ggcaaccgga gtccgcgtgg ggtgtgctgc tcgacaacct gcatgcgctg
    14701 ccaggacatc agggcctcgg cgcgggcaag ctgttgatgc gtgcagccga ggattgggct
    14761 cgcacgcaag gagagacgca gttgtatctt tacgtgctcg aaggcaacac accggcgatc
    14821 ggcttttatg agaaacaagg ctggcagttc gtcggcgccg agccggatca tatgggcggc
    14881 gtggatatta ctgcattgcg ctatgtgtac cggctggatc gatgatgcaa gcgtgcctca
    14941 cttgatcttc gtccaaagcg ggtccgaaat ctggcccggt tggcgcttct ttttcggacc
    15001 attccgatcg ttagttcagc cacttccggg catcatcttc tgtatcccga tggcttcaca
    15061 gtgaccgctc atgcggcggc gtgctcgacc tgagaacgac gtctccgtct tgcgttgtgt
    15121 acggcgtttt cgtacgcatc gcgctgaagc cagagccccc agagccgcct gtccgcggcc
    15181 cctttacgag gcgctgcgaa gaaaccgatg tcgaccacta cccccctcac gctgatcgaa
    15241 ggctccatgg cgctgccgag tctgtcgcca ctgtgccgcc tgcttccccc gccattcggc
    15301 cgcgattggt ccctactgcc tcccgggcac agtttgcgtg cggaactcaa gacgctttat
    15361 gcagccgtgt tacgtacctt ttgtctgaat gcggaagcca tccgcgcgct ctgccagtgg
    15421 gagtcgttgc gcaggccgca cgctgcgtcc tcagggttcg cgtcgcgtgc tcactcgaca
    15481 agtgtggttg acggatcggt cgatgcatcg gttgacggat cattgcgtac ggtcgccgaa
    15541 gccgcacctt tctcgccggt tagtgcacca ggagtaccgg cttcgccaca cggcgggtcg
    15601 aggcattggg gtgcgcttgc gggcggtgct tgcgcactcg gcggcgccgc gatccttgcg
    15661 tggatcgcgg tcggtcatct gacgcagcgc gacacgattg gcgaggtcaa aacagcgggc
    15721 aagttcccgg tccgtcagga agcccagttg gcgaaccatc actcgcccga tgccgctgcc
    15781 acgccttatg caaccgataa tgaccgtgca gcggcgcatg aagttttgcc gaacgcgctt
    15841 gcaacggctc cgctacgcga tctcactaca agtcctgcct cagaaatcgt cacgccagtc
    15901 aaggcagcgc cggcgcgtgc caccaccggc aagaacggat ctcgtcgccg ggacaggcaa
    15961 cgcgaaagcg ccgcgatcca acatgaaaga cgcggccgca attcgcgcac cgccatcaca
    16021 gagaacttgt caccgcaact gccgtcgcag ttgccacatg cgtcgccgat gaactacgaa
    16081 gggtcgcaga ttgcggtgtc ggcaaacgct catcgaacgt caccaaaacc atcgtccgcc
    16141 ggtccttact cgccactcgc gccatcgcaa ctcggcaccg acgaatacgc gtcgatgacc
    16201 ctgtcagctg gcacacacct gcgcaatatt gcgccgccat cacgccctgc ggcgtcggtc
    16261 aattcgcccg gaatcagtgg gactgaatgg atcaatcaca tgtcgcaacg tcgcgtgacc
    16321 gaggatcccg atcagttcgc caactaaccg catcggtatc acagaatcgg cgcgcaaaaa
    16381 acaaaacccc acgctccgaa aaacgtgggg ttggtcagaa gcctgaaccc ggccacgaaa
    16441 aacgccggga tttttcacgc tcgtgcgcct acttctgcgt tggcaatccg aactcggttt
    16501 ggcgctgcaa ctgcatcact tcaccctgca cctggcgcaa ctgcgcctgc gccttttcct
    16561 tctccgcatg cagcgcgacc gcttcgcctt gcgtctgccg ctggtagtcg ttgaccttgg
    16621 cctgctgcgc acgcgcgacg tcgagatcgg cctgcagccg tttcgcgcga tcttccgaca
    16681 aagcgatgat gtcttcgata tacgccttct gtgcctgcaa ctgcgtgcgg cgaatttcga
    16741 cgtccgcgag ttgcgccgtc tgctggacga agccggcata gaccgcttcg gcacgagtcg
    16801 tgtcctgcga tttgatcacg cgccagaaat gcttgtcctg aaacagcgcg acgtagtacg
    16861 tcatttcctg tggatagaaa aacagactcg cgccataact gccgttatac gtagtgcgca
    16921 attcgttcag cttcgcgtcg tgaatcatct gcatcagttc ggcgacgttg ccttgcgcgt
    16981 cattcgcgct agccggcgtg cttgatgccg cattgcgcag tgcggcggcg accggttgcg
    17041 gcgttagcgt gttcatggcc ggccgcgtgc caactagagg tgcggcctcg tcgctctgcg
    17101 ccgccagcac aaaaccacca tgctgcaaca gcgcgcacga ggccgcgagc agcatggcgc
    17161 gccgtacaat taaggggtac ttcatacgtg catgctccat gcaaatccaa aacagccgca
    17221 gattatcgcg cggattagcc gtgaagcgaa gcattcaagt gtcgcgctga cggactttaa
    17281 atcgagataa atcgataacg aatgatttgg ccggatgcac gcacctgtcg cgaaataaaa
    17341 aatttcagat aagacaaaaa cgcaacaatc cgactcatgc agacgtttcc caatctgcac
    17401 ggcgctcgcg cgaatgcaac atcaggcaac gaggaatttt cgagacaagc agccaaataa
    17461 gtagatcgct cgacctgcgc gttattcatt tcgcacaaac gcgatgaagc gaggaccgct
    17521 tcagataggc ctgaagtatc gcgaggcgaa ttctcgggtg acgtgcgcaa cctggtcgat
    17581 aacccactcg ccatcccgac tatcttgata acgcttgata agtcttgata ccgcgccgcc
    17641 gaagaccggc aacgaccaga tgccttctcg acagagaaga gaaacgccag agcgggaaaa
    17701 aaacagcaca cgaggcgtgc cgctaaaaaa gacggcaacg cctcgcgctt tcgggtttga
    17761 aagaccaaac tcgaaagcgc ggctttaagg ccgcagcctt aaaaccgcgt ttcgcgcgcc
    17821 acccgcaaga aggtatcgag caacggcgtg caatcgagca actccggacc gccggcacga
    17881 tgaaactccg ggtgccattg cacacccatc acgaacggcg cgcgccgata gcgcacggcc
    17941 tcgatgatgc cgtccgacgc cgacaccgcc tcgatattca gatcgcggcc gagcgtcttg
    18001 actgcctggt ggtgaatcga gttgacgatc gcatcgcggc ggcccggaaa catattggcc
    18061 agcgttgaac catcgggaaa atgaatgccg tgccgatgct gatcgtagtt ctcgttgacg
    18121 tgcgtcccgg ccgttggcac atcggtggcg atgtcctggt acaagctgcc accgaacgcc
    18181 acgttgatca actggcagcc gcggcacacg ccgagcaccg gcttgcccga ctcgatgaac
    18241 tcatgcagca gttcgagttc gtacatgtcg cgcacacggt cgcccggcca ctcggggctt
    18301 gtcgcctgtt ccgcgtacga ctgcggcgaa acgtccgcgc cgccttgcaa cagcagaccg
    18361 tcgaggtgct tcgcatagtc gcgcagacgg atattgcttg ggtgcagcat gccctgatgc
    18421 ccgaccgtcg gaatcatgaa caccagcaca tcgcgcgaca ttacccagtg cgcgatcgat
    18481 tcctcgagat attgcagcgt tttgccgcgc aaccccttcg cgcccggctc cgggtgaaag
    18541 atgcgcgctg atacgccgat gcgcaaggtg cgctgcgtga tccgttgacc ggcgcgatcg
    18601 aacagctgac gcgcgcgcgc cgcgatgatg cggccgaaca cggtccacgc cgaatcgttt
    18661 tgcttcagat agcgcggcgg cgacggcggc aaagcgttcg gcggtggcgg gttggtcgcc
    18721 gtgaagtcag gtgcggcgcc gaagcctggc ggcggcgagc cagccgggcg agtggctgct
    18781 tcctgcgctt tttccgtgac ggtggcctcg tcaaccgaca cgctcaattc gccggcgagc
    18841 ggttcgctgc tttcgagcgt agccggacgc ggcgcttgaa ccctgctggc atgtcccgct
    18901 ttagccgcgg cgcgctcatg gactgcggcc tcctgctgcg cggtcgacgc ggccgccgca
    18961 ctgcgcgctt cggccgcgtc gcgttgccgc gccatttgcg cgtctgtcgg cgtaacggta
    19021 gacgcgctct gcgggtcgtc ggtcgacgat cttacggccg ccgcttcggc agcaggcgat
    19081 gtacttgacg cagcaggcac gttagccgac gaatctcccg gcaaagcggt cgagcgtggc
    19141 gtgccggata ccgtcggcgt agcggtcgtg cctggcgtcg ccgaaggcga tgctgggccg
    19201 ccgggcttgc cggcgttttc aggtttgttt tcgctcatga cagtcagtca ggcgccttcc
    19261 acgcgaacga atgaatatgc cctgattatc cgctagcggg gcaagccccg caaacccgca
    19321 cacaattgct cacattgttg cggctcaagg cacagcgccg tctaacgcag caccgtctaa
    19381 cttaaggatc cggttgttct tcgaaagttt cgcgcagcgc aatctgcatc gccgcgtgga
    19441 ttttcacgca ccagcgccag accaacgcca gcaacaccgc ggcgcagatc agcaccacgg
    19501 tcaggaggcc cgtcggcggc aagatgctgc cggagagcgc cgcgaccagc aaaaacacgc
    19561 caaccatcga aaccaccggc accaggtcgg aaatcgcgta acggattgca cgggtgaagc
    19621 gtcccgctgt cgccggctgc acgctcacct cggcgagcaa cagcgcaagc gacttggtct
    19681 ttcgatagac cgccaccaga aacggcatcg acagcaccag cgcgatactc cacagcacca
    19741 cgcgctgcat cggctcggaa cttatccagt gcgcgagcca ggtgctgctg tacggcgcgc
    19801 tatacgacac gatcagaaag atcgccgcga ccagcgcgag attgacggcg atctgcaaaa
    19861 tgatccgccg cgtcatgctg aacagcgtcg gctcaccggt ttcggtgctc aggctgcgca
    19921 gccactgccc gtacatgccg aagccgttag cgagcgtgcg tggcatcgcc tgaccgagac
    19981 gctgcgtcag cgggtcggcg gcgcgaatca ggtagggcgt gaaaagcgtg gtcaaggcgg
    20041 agacagccac cgcgatcgga tacagaaacg cgctcgtcac cttcaaggtc aagccgagcg
    20101 acgcgatgat gaacgagaat tcgccgatct gcgaaacggt catgccgacc cgcatcgccg
    20161 tgcgcccatc cttgccggcc agaaacgtgc cgagtccgca cgaaacgatc ttgccgacga
    20221 tcaccgccac cgtgatcacc gcgatcggcc acgcgtagtc gaccagcacg gcggggttca
    20281 gcatcaagcc gatcgtcacg aagaagatcg cggagaaggc gtcgcgcagg ggcccgatca
    20341 gatgctcgat ccgatgcaga tggcgcgact cggccatgat cgcgccgatc aggaacgcgc
    20401 cgagcgcaat gctg
//
LOCUS       DATASH010000038         3764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5939140,
            whole genome shotgun sequence.
ACCESSION   DATASH010000038 DATASH010000000
VERSION     DATASH010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5939140"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="VHV99_01325"
     CDS             complement(<1..391)
                     /locus_tag="VHV99_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3634130.1"
                     /translation="MNPKAHQPDSLPAPGPSALAQSEALVAQIRAELETAGGWLPFDR
                     YMERALYAPGLGYYSGGARKFGLRGDDGSDFVTAPELSPLFAATLGRPVAEALQASGT
                     RDVMEFGAGTGKLAAGLLNALDALGTEF"
     gene            405..1220
                     /locus_tag="VHV99_01330"
     CDS             405..1220
                     /locus_tag="VHV99_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3634131.1"
                     /translation="MTASMDTAARAPETPRIVLITGAARRIGRSLALGFAARGWDVAV
                     HYGASRAEADELVAEIVASGRRAVALHAELGDEAQVERLLPACTAALGRPTCIVNNAS
                     RFEEDTALNVGYDLLLKLTAMNLGAPLVLARMLFEATPEAARTDERQRGVVINVLDQK
                     LYNMNPDYLSYTLSKAALQTATVALAQALAPKVRVVGLAPGLTMQSGDQTPAGFDEAH
                     RATPLGRASRPEDIVAAALYLADAAGVTGTTLVVDGGQHLVPLPRDVMFLTGA"
     gene            1316..1711
                     /locus_tag="VHV99_01335"
     CDS             1316..1711
                     /locus_tag="VHV99_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEX3634132.1"
                     /translation="MFAALSHPRLADCRRLFLRNYEVHINIGVHDFEKRGEQRVVINV
                     ELFVPLALSTPVQDKLTEVVDYDFMRSTIARRVEQGHIHLQETLCDDLVKALLAHPQV
                     RAVCVSTEKPDVYPDCDAVGVEVFRIKED"
     gene            1715..2713
                     /gene="ttcA"
                     /locus_tag="VHV99_01340"
     CDS             1715..2713
                     /gene="ttcA"
                     /locus_tag="VHV99_01340"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEX3634133.1"
                     /translation="MNAQETLNGGAAPEAAQVAHASAGKAPLTRREQKEAYENNKLFK
                     RLARQVGEAIGDFNMIEEGDKVMVCLSGGKDSYAMLEILMRLRERAPINFDIVAVNLD
                     QKQPGFPEHVLPEYLKQLDIPFHIENQDTYSIVKRLVPEGKTTCSLCSRLRRGILYRV
                     AGELGATKIALGHHRDDILQTLLLNMFYGGKLKGMPPKLQSDDGKNIVIRPLAYVKET
                     DLEKYAELREFPIIPCNLCGSQPNLKRAEMKALIRDWEKRFPGRIENMFNALSNIVPS
                     HLMDHKLFPFASLRATGEADPQGDIAFDEEPCSTDTGDGARLGGSKPISIVQFDDM"
     gene            2867..>3764
                     /locus_tag="VHV99_01345"
     CDS             2867..>3764
                     /locus_tag="VHV99_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011353430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEX3634134.1"
                     /translation="MNIVILAAGTGKRMRSALPKVLHPLAGRPLLAHVIDTARTLKPT
                     RLVVVVGHGADAVRQAVAAPDVQFAAQEQQLGTGHAVQQALPLLDPSQPTLVLYGDVP
                     LTRAATLQALTDRAGHGGYGVLTVTLDNPSGYGRIVRDQHGKVSRIVEQKDATPEQLK
                     IAEINTGIIVAPTERLGEWLAALKNDNAQGEFYLTDAVEMAIEAGLEVVTTQPDDEWE
                     TLGVNSKQQLAELERIHQRNVADALLVAGVTLADPARLDVRGPLECGRDVSIDVNCVF
                     EGRVTLADNVTIGPNCVIRDANI"
BASE COUNT          705 a         1212 c         1197 g          650 t
ORIGIN      
        1 cgaactcggt gcccagcgcg tcgagcgcgt tgagcaagcc agcggccagc ttacccgtgc
       61 cggcgccgaa ttccatcacg tcgcgcgtgc cgctcgcctg caaggcttcc gcaaccgggc
      121 ggcccagcgt cgcggcaaac agcggcgaca gttccggagc ggtgacgaag tcgctgccgt
      181 cgtcgccgcg cagcccgaat ttgcgggcgc cgccgctgta atagccgagt cccggcgcgt
      241 acagcgcgcg ctccatgtag cggtcgaacg gcagccagcc gccggccgtt tcgagctccg
      301 cgcggatctg tgcgaccagc gcttcggact gcgcaagcgc gctcgggccg ggagcaggta
      361 aactatcggg ttggtgagct ttcggattca tccccgcatt gtaaatgacc gcctctatgg
      421 ataccgccgc tcgcgcgcct gaaacgccgc gcatcgtgct gattaccggc gccgcccgcc
      481 gcattgggcg ctcgctagcg ctcggctttg ccgcgcgtgg ttgggacgtc gcggtccatt
      541 acggggcgtc gcgggccgaa gcggacgaac tcgtcgcgga aatcgtcgcc tcgggccgcc
      601 gggcagtggc tttgcacgcg gaattgggcg acgaagccca ggtcgagcga ttattgcccg
      661 cctgtaccgc cgcgctgggc cgtccgacct gtatcgtcaa caacgcgtcg cgcttcgaag
      721 aagatacggc gcttaacgtc ggttacgacc tgctgctgaa gctcaccgca atgaatctcg
      781 gcgcgccgct cgtgctggcg cgcatgctgt tcgaggctac gccggaggcc gcgcgcaccg
      841 acgagcgcca gcgcggcgtc gtcatcaacg tgctggatca gaagttgtac aacatgaatc
      901 cggattacct gtcgtacacg ctgtcgaaag cagcgttgca gacagcgacg gtcgcactgg
      961 cgcaggcgtt ggcgccaaaa gtgcgcgtgg tgggcctcgc gcccggtctg acgatgcaat
     1021 ccggcgatca aacgccggca ggttttgatg aagctcaccg tgctacgcct ctgggccgcg
     1081 catcgcggcc ggaggatatc gtcgccgccg cgctgtatct cgccgatgca gcgggcgtca
     1141 ccggaacgac gctggtggtc gacggtgggc agcacctggt gccgctgccg cgcgacgtga
     1201 tgtttttgac gggcgcctga accgcgcgcg cttgttgccg ggtcgaaccg gcagccgccc
     1261 gtgcgctgaa gcgtcccctt gcgtgccccg cacgcttttt tcgactggaa cgaacatgtt
     1321 tgccgctctt tcgcatcccc ggctcgccga ttgccgccgg ctctttctgc gcaattacga
     1381 agtgcacatc aacatcggcg tgcacgactt cgaaaagcgt ggcgaacagc gcgtcgtgat
     1441 caacgtcgaa ctgttcgtgc cgctcgcgct gtccacgccg gttcaggaca agctgaccga
     1501 agtcgtcgat tacgacttca tgcgctcgac catcgcgcgt cgcgtcgaac aaggtcatat
     1561 tcacttgcag gaaacgctct gcgacgacct cgtcaaggcc ttgctcgctc atccgcaggt
     1621 gcgtgccgtg tgcgtgtcga ccgaaaagcc ggacgtttat cctgactgcg acgcggtggg
     1681 cgtcgaagtc ttccgcatca aagaggattg agccatgaat gctcaggaaa ccctgaacgg
     1741 cggcgcggcc cctgaagctg cacaggtcgc gcacgcctca gcgggcaaag cgccgctcac
     1801 gcgccgcgag cagaaagaag cctacgagaa caacaagctg ttcaagcgcc ttgcgcgtca
     1861 ggtcggtgag gcgatcggcg acttcaacat gatcgaggaa ggcgacaagg tgatggtctg
     1921 cctgtcgggc ggcaaagaca gttacgcgat gctcgaaatc ctgatgcggc tgcgcgaacg
     1981 cgcgccgatc aacttcgaca tcgttgcggt gaatctcgac cagaagcagc cgggctttcc
     2041 ggagcatgtg ctgcccgaat acctgaagca actcgacatt ccgtttcaca tcgagaacca
     2101 ggatacgtac agcatcgtca agcggctggt gccggaaggc aagaccacct gctcgctgtg
     2161 ctcgcggctg cgccgcggca ttctgtatcg cgtggcgggc gaactcggcg cgaccaagat
     2221 cgcgctcggc catcatcgcg acgacatcct gcaaacgctg ctgctgaaca tgttctacgg
     2281 cggcaagctg aagggcatgc cccccaagct gcaatcggac gacggcaaga acatcgtgat
     2341 ccgcccgctc gcctatgtga aggaaaccga tctggagaag tacgcggagc tgcgcgaatt
     2401 cccgatcatt ccgtgcaatc tgtgcggcag ccagccgaac ttgaagcgtg cggagatgaa
     2461 ggcgctgatc cgcgattggg agaagcgctt tccgggccgc atcgagaaca tgttcaatgc
     2521 gttgtcgaac atcgtgccgt cgcacctgat ggaccacaag ctgttcccgt ttgccagcct
     2581 gcgcgccacg ggcgaggccg atccgcaagg cgatatcgcc ttcgacgaag agccgtgttc
     2641 gaccgatacc ggcgatggtg cgcgcctcgg tggatcgaag ccgatctcga tcgtccagtt
     2701 cgacgatatg tgagagacac ggaaacaggc gaaaaacaca cggaaaacgt gcgggaaatg
     2761 tgggatttgg cgcatttcta cccactttga gccgcgcgag cggcctttgt cgtcataggc
     2821 tgcatgatag aatcgtcgcc tccaaacttg tctatttgcc gacgccatga acatcgtgat
     2881 tttggcggca ggcaccggca agcgcatgcg gtccgcgctt cccaaggtgc tccatcccct
     2941 ggctggccgg ccactcctcg ctcatgtcat cgacactgcc cgcacgctca agcccacgcg
     3001 gctcgtcgtg gtggtcgggc atggcgctga cgccgtgcgt caagcggtag cggcgccgga
     3061 cgtccagttc gccgcgcagg agcaacaact cggcacgggc cacgcggtgc aacaggcgct
     3121 gccgctgctc gatccgtccc aacccacgct cgtgctgtac ggcgatgtgc cgctcacacg
     3181 tgccgctacg ctgcaggcgc tgaccgatcg cgcgggccac ggcggctacg gtgttctcac
     3241 cgtcacgctg gacaacccta gcggctatgg ccgcatcgtg cgcgatcagc acggcaaggt
     3301 gtcgcgtatc gtcgaacaga aagacgcaac gcccgagcag ctcaagatcg ccgagatcaa
     3361 caccggcatc atcgtcgcgc cgaccgagcg tcttggcgag tggctcgcgg cgttgaagaa
     3421 cgacaacgcg caaggcgagt tctacctgac cgacgcggtc gagatggcaa tcgaagcagg
     3481 gctcgaagtc gtcaccacgc agccggacga cgagtgggaa acgctcggcg tgaacagcaa
     3541 acagcagctt gccgaactcg aacggattca ccagcgcaac gttgccgacg cgttgctggt
     3601 cgcgggcgtg acactcgccg atccggcgcg gctcgacgtg cgcggcccgc tcgaatgcgg
     3661 ccgcgacgtt tcgatcgacg tgaactgcgt gttcgagggc cgcgtgacgc tggccgacaa
     3721 cgtcaccatc ggaccgaact gcgtgatccg cgatgcgaac atcg
//
LOCUS       DATASH010000039         4067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3763500,
            whole genome shotgun sequence.
ACCESSION   DATASH010000039 DATASH010000000
VERSION     DATASH010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3763500"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..1226)
                     /locus_tag="VHV99_01350"
     CDS             complement(120..1226)
                     /locus_tag="VHV99_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3634135.1"
                     /translation="MKTDDVIVSFRGVRKTYDGETLVVKQLDLDIYQGEFLTLLGPSG
                     SGKTTCLMMLAGFEFPTGGEIWLDGTLLNAVPPHKRNIGMVFQNYALFPHLTVEQNVA
                     YPLTVRKLSADERAHRTNNALKMVRMESFAKRYPAQLSGGQQQRIALARALVFEPKLV
                     LMDEPLGALDKQLREHMQYELKSLHEKLGVTFVYVTHDQGEALTMSDRVAVFDKGIVQ
                     QLDTVDRLYESPCNEFVANFIGDSNKLRGTIANVDGEYCEFHLIDGTRLTGRNIGGAR
                     AGTPAVACIRPERMKLASLVNGASRPGANALSGEARGLIYFGDHVRMRCGLPEQDECF
                     VKVPLGTDALETFAPGAPVALEFAPEHLRVFAGA"
     gene            1355..2896
                     /locus_tag="VHV99_01355"
     CDS             1355..2896
                     /locus_tag="VHV99_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX3634136.1"
                     /translation="MDTVILSDWLAARLDRTAAEPVYRQTLRLMQQAILTGQLPPGTK
                     LPSSRMLAEDLGVARNTVLHVYDQLTAEGYVISTTGSGTYVADTRPDTAAVNARKKPA
                     VVSVDEHEEAVQKPPKRDLGDLSMRGRRLIDQAGVSAKQWGAFMPGVPDVAEFPARTW
                     SRLQARLWKEANPDLLTYAPGGGYRPLRRALSDYLRVARSVNCTPDQIIITTGIHQSI
                     DLAVRLLTDVGDRAWVEEPCYWGARSVLQSSGITLVPVPVDDEGLNPREQDLQQPPRL
                     ALVTPSHQYPLGMVMSLARRRTLLEYARQHNVWIIEDDYDSEFRYGSRPLASLQGLDD
                     AGQVIYVGSLGKMLFPGLRIGYMIAPAHLVDTFRTGVAELYREGQLMQQAVMTDFIMD
                     GHLTSHVRRMRALYGERRQILIDAITARFGNELPVMGDEAGLHLVLGLPDHANDRAVT
                     AAAYDAGVIVRPLAAYYSSETPARRGLLLGYACVANEKIGPAFDTLARVIEQTALKKR
                     PTRAA"
     gene            complement(2927..>4067)
                     /locus_tag="VHV99_01360"
     CDS             complement(2927..>4067)
                     /locus_tag="VHV99_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HEX3634137.1"
                     /translation="YTDNFIKPNYTANLVGIQGTVGAFGTQSTASAPVDIAAKLAANG
                     PLSIRGTVNPLIAKPALDLTASAHDIELTNLTPYSSKYAGYPITKGKLNVDLHYKLDN
                     DQLNADNHLFIDQLTFGDHIDNDTATKLPVRLAISLLKNSRGEIDVNLPVSGSLSNPE
                     FSVGGLIWHAVLNLLQKAVTAPFSLIGHAFGGSGEELGYVEFEPGSAKLTDADTKKLD
                     TIVKALADKPSIRMDLIGRVDPAVDEPALRTGYVDRLVKQQKIKAVVGNGESVDLSTV
                     TVDAKDYDKYLTQAYKSADFKKPRNFVGLTKSLPADEMKSALAANAPIDDASLRALAQ
                     QRAQSVQQYLDGKIDSSRVFIVAPKLNADGIQDKGATTRVDFGLK"
BASE COUNT          731 a         1266 c         1275 g          795 t
ORIGIN      
        1 gtacggcttg ttatgtgctg ctgactcgac ctgcctgaat ttgacccact acgttggacc
       61 cactgcgccg aacgatccgc atcgaacgaa ctccgtcgat cgtttcgcgc caaacacctt
      121 cacgccccgg caaaaacccg cagatgctcg ggtgcaaact ccagtgcaac cggcgcaccc
      181 ggcgcgaagg tttcgagtgc atcggtgccg agcggcacct tgacgaagca ctcgtcctgt
      241 tccggcaggc cacagcgcat acgcacgtgg tcgccaaaat agatcagccc gcgcgcttcg
      301 ccgcttagcg cattggcgcc cggtcgtgac gcgccattca ccaggctggc gagcttcatg
      361 cgctcaggcc ggatgcaggc aaccgccggc gtgcccgccc gcgcgccacc gatattgcgt
      421 cccgtgaggc gggtgccgtc aatcagatga aactcgcagt actcgccatc gacgttcgca
      481 atcgtgccgc gcagcttgtt gctgtcgccg atgaagttcg cgacgaactc attgcacggc
      541 gattcgtaca gacggtcgac ggtatcgagc tgctggacga tgcctttatc gaacacggcg
      601 acgcggtcgg acatggtcag cgcctcgccc tgatcgtgcg tcacatagac gaacgtcacg
      661 ccgagcttct cgtgcaatga tttaagttcg tactgcatat gctcgcgcaa ctgcttgtcg
      721 agtgcgccga gcggctcgtc catcaagacg agcttcggtt cgaacacgag cgcccgcgcc
      781 agcgcaatac gttgctgctg accgccggaa agctgcgccg gatagcgttt cgcgaagctc
      841 tccatacgca ccatcttcag cgcgttattc gtgcgatggg cgcgctcatc cgcggaaagt
      901 ttgcgcaccg tgaggggata cgcgacgttc tgctcgaccg tcaaatgcgg aaacaaagcg
      961 tagttttgaa acaccatgcc gatgttccgc ttatgcggcg gcacggcgtt gagcagcgtg
     1021 ccgtccagcc agatctcgcc gccggtggga aattcaaagc ccgccagcat catcagacat
     1081 gtggttttcc ctgatcccga gggtccgagc agcgtcagga attcgccctg atagatatcc
     1141 agatcgagtt gtttgacgac cagcgtttcg ccgtcgtatg tcttgcgcac acctcgaaag
     1201 ctgacgatca cgtcatcggt tttcatgcct tcgtctcctc tgcggttcgg ctggctcccc
     1261 tgcaaaacag agggatagcg tcaattgcgt ggctcactat agtcccttac ggttctacac
     1321 tatggaacca tttcattatt ccggatagaa ccacttggac accgtgatct tgtcggattg
     1381 gctcgccgcg cggcttgacc gcacggccgc cgagccggtc tacagacaga ccctaaggct
     1441 gatgcaacag gccatcctga ccggccagtt gccgcccggc accaagctgc cgagttcgcg
     1501 catgctcgcc gaagacctcg gtgtcgcgcg caacacggtg ctgcacgtct acgatcagtt
     1561 gaccgccgaa ggctacgtga tctcaaccac cggcagcggc acctatgtcg ccgacacgcg
     1621 gccggacacg gcggcggtga atgcgcgcaa gaagccggcg gtggtgagcg tggacgagca
     1681 cgaggaagcg gtgcagaagc cgcccaaacg cgatctcggc gacctctcca tgcgcggacg
     1741 aaggctgatc gatcaggctg gcgtctccgc caaacagtgg ggggcgttca tgccgggcgt
     1801 gcccgatgtc gccgagtttc cggcgcgcac atggagccgc ttgcaggcaa ggttgtggaa
     1861 ggaagccaat cccgatctgc tgacttatgc gcctggcggc ggctatcggc cgctgcggcg
     1921 cgcattgtcc gattatctgc gcgtcgcgcg ctcggtgaat tgcacgcccg atcagatcat
     1981 catcacgacg ggcattcatc agtcgatcga tctggcggtg cgtttattga ccgacgtcgg
     2041 cgatcgcgca tgggtggaag agccgtgcta ttggggtgcg cgcagcgtgc tgcaatcgtc
     2101 ggggatcacg ttggtgcccg tgcctgtgga cgacgagggt ctcaacccgc gcgaacagga
     2161 tctgcagcag ccaccgcgat tagcgctcgt cacgccctcg catcaatatc cgctcggcat
     2221 ggtgatgagt ctcgcgcgcc gccgcacgct gctcgagtac gcgcgccagc acaacgtctg
     2281 gatcatcgag gacgactacg acagcgagtt ccgctacggg agccgtccgc tagcatcgtt
     2341 gcaaggactc gatgacgcgg gacaggtgat ttacgttggc agcttgggga agatgctgtt
     2401 tcctggcttg cggatcggct acatgatcgc gccggcgcat ctggtggata cgttccgcac
     2461 gggcgtggcc gagctgtatc gcgagggaca attgatgcag caggcggtga tgaccgactt
     2521 catcatggac ggccatctca cgtcgcacgt tcgccgtatg cgcgcgctat atggtgaacg
     2581 cagacagatt ctgatcgacg cgatcaccgc gcgtttcggc aacgaactcc cggtgatggg
     2641 cgacgaagcc ggcctgcatc tggtgctcgg cctgccggat catgcgaatg atcgcgcggt
     2701 aaccgctgcg gcttacgacg ctggagtgat cgtgcggccg ctcgccgcct actacagcag
     2761 cgagacgccc gcgcggcgcg gcctattgct gggatacgcc tgcgtggcga acgagaagat
     2821 cggtccggcg ttcgacacgc tcgcgcgggt gatcgagcag acggcgttaa agaaaaggcc
     2881 aacgcgcgcc gcctgagcgc acaccgccac tgcgcggcaa cgcgcctcac ttcaagccga
     2941 agtccacgcg cgtggtcgcg cccttgtcct ggatgccgtc ggcgttcagc ttcggcgcga
     3001 cgatgaacac gcggctgctg tcgatcttgc cgtccagata ctgctgcacg ctctgcgcgc
     3061 gctgttgcgc aagcgcgcgc aagctggcgt cgtcgatcgg cgcgttggcg gcgagcgcac
     3121 tcttcatctc gtcggccggc aagctcttgg tcaggccgac gaagttgcgt ggcttcttga
     3181 agtccgcgga cttgtaggcc tgggtcagat acttgtcgta atccttggca tcgacggtca
     3241 cggtcgacag atcgacgctc tcgccgttgc ccaccacggc cttgatcttc tgctgcttca
     3301 cgagccggtc gacatagccg gtgcgcaacg ccggttcgtc gactgcaggg tcgacgcggc
     3361 cgatcaggtc catccggatc gacggcttgt cggcgagtgc cttgacgatc gtgtcgagct
     3421 ttttggtgtc ggcgtccgtg agcttggccg aacccggctc gaactcgaca tagcccaact
     3481 cctcgccgct gccgccgaac gcgtgaccga tcagcgagaa cggcgcggtc accgctttct
     3541 gcagcagatt gagcaccgca tgccagatca ggccgccgac gctgaactcg ggattcgaca
     3601 gcgaacccga caccggcaga ttcacgtcga tctcgccgcg cgagttcttc agcagcgaga
     3661 tcgcgagacg caccggcagc ttggtcgccg tatcgttgtc gatgtggtcg ccgaacgtga
     3721 gctggtcgat gaacaggtgg ttgtccgcgt tcaactgatc gttgtcgagc ttgtagtgca
     3781 ggtcgacgtt cagcttgccc ttggtgatcg gatagccggc gtatttcgac gaatacggcg
     3841 tgaggttggt cagttcgata tcgtgcgcgc tcgcggtcag atcgagcgcc ggtttggcga
     3901 tcagcggatt gacggtgccg cgaatcgaca gcggaccgtt ggccgccagc ttcgcggcga
     3961 tgtcgaccgg cgcagaggcg gtcgattgcg tgccgaacgc gccgaccgtg ccctgaatgc
     4021 cgaccaggtt cgcggtgtag ttgggcttga tgaagttgtc cgtgtac
//
LOCUS       DATASH010000040         3766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2181207,
            whole genome shotgun sequence.
ACCESSION   DATASH010000040 DATASH010000000
VERSION     DATASH010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2181207"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1028..1732
                     /locus_tag="VHV99_01365"
     CDS             1028..1732
                     /locus_tag="VHV99_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HEX3634138.1"
                     /translation="MINKIFESLQSAVADVPDGATVMIGGFGTAGMPSELIDALIEQG
                     ARELTIVNNNAGNGDIGLAALLKAKRVRKIICSFPRQTDSYVFDALYRAGEIELELVP
                     QGNLAERIRAAGAGIGGFFTPTGYGTKLAEGKETRLIDGKHYVLEAPLHADFALIKAL
                     KGDRWGNLVYRKTARNFGPIMASAAKTAIVQVSKVVPLGELDPENIVTPGIFVQRVIE
                     VPQATHQAELASATAA"
     gene            1744..2403
                     /locus_tag="VHV99_01370"
     CDS             1744..2403
                     /locus_tag="VHV99_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEX3634139.1"
                     /translation="MKKLTRDEMAKRVAQDIPEGAYVNLGIGVPTLVANHLAADKEIF
                     LHSENGLLGMGPAPAKGEEDDELINAGKQHVTLLTGGAYFHHADSFAMMRGGHLDFCV
                     LGAFQVSAKGDLANWHTGAPDAIPAVGGAMDLAIGAKQVYVMMEHLTKQGESKIAAEC
                     TYPVTGVGCVDRIYTDLAMIDVTDKGLVVREIFSDLDFDALHKLTGVPLIDGTQTARA
                     A"
     gene            2979..>3766
                     /locus_tag="VHV99_01375"
     CDS             2979..>3766
                     /locus_tag="VHV99_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEX3634140.1"
                     /translation="MLDSSARLTGLLCGTQPMNDIWAPRATLQRMLDVEAALARASAS
                     HNVIPQSAVAAIEAACQADQLDADALARDAALGGNLAIPLVKQLTARVKTADAEASKY
                     VHWGATSQDIIDTATVLQLRDTFDLLDSGLQATCDAVARLAAAHRATPMIGRTWLQQA
                     LPITLGLKFAQWLDALLRHRERLDALRARVLVLQFGGAAGTLASLRDAAPQVTQSLAR
                     ELKLAVPTLPWHTQRDRIAETAALFGMLIGTLGKIARDISLQMQT"
BASE COUNT          814 a         1204 c         1160 g          588 t
ORIGIN      
        1 gaaagcggct cacaccgcta gcgcataagc gggtcccccg cacagtgacg gtagtggtgc
       61 atctggaatc tgtgccctcg cgcattcagc cctggtgacc aggcagtcat acttcccgcc
      121 tcgccctgcg tgctcgccgg aagggacgat catgcaaacc agtggcttcg tctggaggtg
      181 atggggccgt cggcttcgcc tcggcgaggc gccccggcga cagcagcagg cgagaagatc
      241 gacagaagcg aagaagcgaa gaggtgaaga ggtgaagagg tgaagaggtg aagaggtgat
      301 acacggcgcc ggatgaaaca tgaccgggaa aacgcggcgg atggttttat gaaatgcgtg
      361 tcggcgcgcg cagcgccgcc ggaagtatga cggctgtgtc accagggccg aatgtgcgag
      421 gggacggatt ccagatgcac cactgccgca actgtgcggg ggacccgctt atgagctggc
      481 ggtttgagcc gctttctttt gcctactttt ctttgcggca ggcaaagaaa agtaggtgcc
      541 tgccccgcac aggggcgacg ctaatagacc aatatcaaat caacgaaagg ccaacgccgc
      601 aggcacacgg accaacaagc gcggcgcagg caaccagtgc aaggaaaggc caacgccaca
      661 ggcacacaga ccaacaagcg ccgcgcaggc aaccagcgca aggaaaaccc accgccggag
      721 gcaaaccaaa caaaaagcgt cgcgcagaca cccccgccgg gcgggccaga acctatgatt
      781 aaggggaatc ccctgccttg acaacccgcc cgaagggcaa ccataatccc gtaaccgttc
      841 gctaatcgaa tccatgttcg catatagaac aaatcgacca acgaacaccc caaaggcgcc
      901 tgcccgcatc cccacccggc gagccgccgc cagtacaggc ccggagaccg tcccgcaaca
      961 agcccctgcc tgcacaggca aaggcaaaaa gcccagcggc acgcggcgcc ccaaaaggaa
     1021 attcgacatg atcaacaaga ttttcgagtc acttcaatcg gcggtcgccg atgtccccga
     1081 cggcgcgacc gtcatgatcg gcggcttcgg cacggccggc atgccgtccg agctgatcga
     1141 cgcgctcatc gaacaaggcg cgcgcgaact caccatcgtc aacaacaacg ccggtaacgg
     1201 cgacatcggc ctcgcggcgc tgctcaaagc caaacgcgtg cgcaagatca tctgctcgtt
     1261 cccgcgccaa accgattcgt atgtgttcga cgcgctctat cgcgccggcg aaatcgagct
     1321 cgaactggtg ccgcaaggca acctggcgga acgcatccgt gccgcgggcg caggtatcgg
     1381 cggcttcttt acgcccaccg gttatggcac gaagctcgct gaaggcaagg agacgcgtct
     1441 gatcgacggc aagcattacg tgctggaggc gccgctgcat gcggacttcg cgttgatcaa
     1501 ggcgctcaag ggcgaccgct ggggcaacct ggtctatcgc aagaccgcac gcaacttcgg
     1561 cccgatcatg gcgagcgcgg caaaaacggc catcgtgcaa gtgtcgaaag tggtgccgct
     1621 cggcgagctc gatccggaaa acatcgttac gcccggcatt ttcgtgcaac gcgtgatcga
     1681 agtgccgcaa gcgacgcatc aagccgaact tgcatccgcg accgccgcct gaggagaccc
     1741 gacatgaaaa aactgacccg cgatgaaatg gccaagcgcg ttgcccagga tatccctgaa
     1801 ggcgcttacg tgaacctcgg catcggtgtg cccacgctgg tggccaacca cctcgccgcc
     1861 gacaaggaaa tcttcctgca cagcgaaaac ggtttgctcg gcatgggccc ggcacccgcc
     1921 aaaggcgagg aagacgacga actgatcaac gccggcaagc agcatgtcac gctgctgacg
     1981 ggcggtgcgt acttccatca cgcggattcg ttcgcgatga tgcgcggcgg tcatctggat
     2041 ttctgcgtgc tcggcgcgtt ccaggtgtcg gcgaagggcg acctggcgaa ctggcacacc
     2101 ggcgcgcccg acgcgattcc ggctgtcggc ggcgccatgg atctggccat cggcgcgaag
     2161 caggtctacg tgatgatgga acatctgacc aagcagggtg aaagcaagat cgccgctgag
     2221 tgcacctacc cggtgacggg cgtgggctgc gtcgaccgca tctatacgga cctggcgatg
     2281 atcgacgtca ccgacaaggg tctggtggtg cgcgagatct tctcggacct cgacttcgat
     2341 gcactgcaca aactgaccgg cgtgccgctg atcgacggca cgcaaacggc gcgcgcggcc
     2401 taagcatcgg cgcggtagca gtcagcataa agcttgcgat aggtgggaat gctggcgcgg
     2461 cggcggcaac gcttacgcgg aaaccgcaaa gcttatgcgg cggcggtaac gcttacgcgg
     2521 aaaccgtaaa gcttatgcgg cggcagcaac gcttatgcgg aaactgcaaa gcttacgcgg
     2581 cggcgacaac gcttatgcgg aaactgcaaa gcttacgcgg cggcggcaac gcttacgcgg
     2641 aaaccgcaaa gcttacgtgg caactgcaac gtttacgcgg caaccggaat gctggcgcgg
     2701 cgaccgcaac cctcacgtcg atccgcgccg tgcacgggtg ccaccaggca tgtccggaac
     2761 gcgcattcgg caagcttcat gcgcgtgttc cgatgtcacg gcggtacgcc gggccatcgc
     2821 gccggtatcg aagcagaatg cgctgcaacg ccgcgtgcgg tacgctctcg atagatagcg
     2881 cgttgggaca cgctgggtgc gccttcggtg tgcgtccacg cgtgtccaga tcgccctggt
     2941 gaaaagcctt tactttcgac tccctcatcg acgccatcat gctcgactcc agcgcccgtc
     3001 tgaccggcct tctttgcggc acacagccga tgaacgacat ctgggcgccg cgtgccacgc
     3061 tgcaacggat gctcgatgtc gaagcagcgc tcgcgcgcgc ttcggcttcc cataacgtga
     3121 ttccacaatc cgccgtggcc gcgatcgagg cagcctgcca ggctgatcaa ctcgacgccg
     3181 acgcgcttgc gcgcgacgcg gcactcggcg gcaacctcgc gattcccctc gtcaaacaac
     3241 tcaccgcgcg cgtcaaaacg gcggacgcgg aagcatcgaa gtacgtccac tggggcgcga
     3301 cgagccagga catcatcgat accgcgacgg tgctgcaact acgcgacacc tttgatctgc
     3361 tcgacagcgg cctgcaagcc acgtgcgacg cagtcgcaag actcgcggcc gcccatcgcg
     3421 cgacgccgat gatcggccgc acctggttgc agcaggcgct gcccatcacg ctcggcctga
     3481 aattcgcgca atggctcgac gcgttgttgc ggcatcgtga gcggctcgat gcattgcgcg
     3541 cgcgcgtgct ggtgctgcaa ttcggcggtg cggcgggcac gcttgcgagc cttcgtgacg
     3601 cagcgcccca agttacgcaa tcattggctc gagaactcaa gcttgctgtg cccaccttgc
     3661 cgtggcacac gcaacgcgat cgcattgccg aaaccgcggc gctgttcggc atgctgatcg
     3721 gcacgctcgg caaaatcgcg cgcgatatct cgttgcagat gcaaac
//
LOCUS       DATASH010000041         4692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5346030,
            whole genome shotgun sequence.
ACCESSION   DATASH010000041 DATASH010000000
VERSION     DATASH010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4692
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5346030"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VHV99_01380"
     CDS             complement(<1..467)
                     /locus_tag="VHV99_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX3634141.1"
                     /translation="MEILGKEAMKTIKRALSGVLALSGLLFAGAALAVNDSPGGPAVN
                     ELNFQTPVTKIAEELYSLHTFMLIICTVIFLGVFGVMFYSVLMHRKSKGHKASNFHES
                     TTVEIIWTIVPFVIVVLMALPATKAVVAMKDTTNADLTVKVTGYQWKWGYDYV"
     gene            complement(651..1127)
                     /locus_tag="VHV99_01385"
     CDS             complement(651..1127)
                     /locus_tag="VHV99_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010101442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HEX3634142.1"
                     /translation="MQATDLPMDTEPVVKDWLIKRNCSVSPRQFVAFYVSLALFSLLI
                     AGLLVWAGAWLVLPFTGIELLAVGVAFVIYARHAVDYERILLFHDRLLIERMDAETLT
                     QMEFNPRWVRVEPGATPRDPVKLVSRGESVPVGIHLAQHRRKQFAVELRMWLRRCG"
     gene            complement(1443..2414)
                     /locus_tag="VHV99_01390"
     CDS             complement(1443..2414)
                     /locus_tag="VHV99_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX3634143.1"
                     /translation="MSPTPANAGRPAYDLRHLQRIFDRRAATFDDVAFLPREIAQRMR
                     ERLDYIKVTPVRVLDAGCGMGADLAGLRERFADASVLGMDLSAAMLARANQAEMAESE
                     ANAGWRRFLPATLGKAFGARGPQLAQGDFAHLPFVASAFEMLWSNLALHWHARPDLVF
                     PEWARVLKVNGLLMFSTLGPDTLKELSGAYAQAEKSLGLTPQRHTIEFVDMHDLGDML
                     VESGFEIPVMDQEVLTITYKSPESLLKDVRRWGAYPRDLAPQTQARALRDALHAALEA
                     RRREDGTIPLTFEVIYGHAWKAVPRVTAEGHGIVRLEDIGRGSKRSK"
     gene            2583..3386
                     /locus_tag="VHV99_01395"
     CDS             2583..3386
                     /locus_tag="VHV99_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEX3634144.1"
                     /translation="MKLRFGVVWRAPGSLRKASATALRRLGQIRDIALPSQCALCGNL
                     SQKVLCAGCDEQYWNQARTRCPVCALPVPVLAAASAASAGFKNGAYLPCRRCIAEPPH
                     FDATIALADYHAPLDTLALDLKFHARLATADEFARQLQAAFESTDAAAPDVVAPVPLS
                     PRRLRTRGYNQAWSIGKPLARRLGSLADATLIERTRDTAPQAKLDLDTRRRNVGNAFR
                     VLRDIRGQHVAIVDDVMTSGATLDALAFTLKAAGARRVTNFVALRTPQD"
     gene            3408..3878
                     /gene="trmL"
                     /locus_tag="VHV99_01400"
     CDS             3408..3878
                     /gene="trmL"
                     /locus_tag="VHV99_01400"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEX3634145.1"
                     /translation="MFNVVLVEPEIPPNTGNVIRLCANTGARLHLIEPLGFPLDDAKM
                     RRAGLDYHEYAQMHVHANWEAFLEKEMPDVSRMFAFTTRGSTPFFGHNFKAGDWFVFG
                     AETRGLSDAVLTEFTNDRRVRLPMRPGNRSLNLSNTVAIVTFEAWRQTGFEGGE"
     gene            complement(3911..>4692)
                     /locus_tag="VHV99_01405"
     CDS             complement(3911..>4692)
                     /locus_tag="VHV99_01405"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011546282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX3634146.1"
                     /translation="DALCVVAAPVAGLRALCRNMRELGIVPAHIVWLCKGFEADTQCL
                     PNEVVAAELPQQASNGTLSGPSFAREVGQGLPVALTIASASATLGERTVAAFHHGAMR
                     IYTGDDVIGVEVGGAVKNVLAIATGIADGLGLGLNARAALITRGLAEMSRLGVALGGH
                     AETFTGLTGLGDLILTATGDLSRNRTVGIQLATGRSLTDILAALGHVAEGVRCARAVL
                     ALAESRGIEMPITHAVCRVLFEDVAPRDAVHALLSRDAKAE"
BASE COUNT          912 a         1521 c         1326 g          933 t
ORIGIN      
        1 ttcacgtagt cgtaacccca cttccactga tagccggtga ccttcacggt caggtcggcg
       61 ttggtagtgt ccttcatggc gacgacggcc ttcgtagcag gcagcgccat cagcacgacg
      121 atcacgaacg gaacaatcgt ccagatgatt tcgacggtcg tgctttcgtg gaaattcgaa
      181 gccttgtgtc ctttcgactt ccggtgcatc aggaccgaat agaacatcac gccgaacacg
      241 ccgaggaaaa tcaccgtaca gatgatcagc atgaacgtat ggaggctgta gagctcttcg
      301 gcgatcttcg tcaccggcgt ctggaagtta agttcgttaa ctgcaggacc gccaggactg
      361 tcgttgaccg ccagggctgc gccggcgaac aggagtccgc ttaacgccag cacgcccgag
      421 agggctcgct tgattgtttt catagcttcc ttacccaaaa tttccattca gaccctcgac
      481 ccccgtatgc acccgtccga ctcatgcatc gcctcccctg gaaacgatct attcggcggc
      541 gcgtgtcgat gcgtgtgccg cttgctacga ccttccatgc ttcctgcttt tatgccgggc
      601 actgccagcc ggacgtcctg aatgcgcccg gacaacccat tttcaggccg tcagccgcag
      661 cgtctcagcc acatccgcaa ctcgacggca aactgcttgc gacgatgctg cgccagatgt
      721 atcccgaccg gcaccgactc cccccgcgac accagcttca ccggatctct cggcgttgct
      781 cccggctcga ctctcaccca acgcggattg aactccatct gcgtgagcgt ttccgcatcc
      841 atccgctcga tcagaagccg atcgtgaaac aacagaatcc gctcgtaatc gacagcatgc
      901 cgcgcataaa tgacgaacgc gacaccaacc gccagcaatt cgatacccgt aaaaggcagc
      961 accagccaag cgcccgccca caccagcaaa cccgcgatca gcaacgaaaa cagcgcaagc
     1021 gacacgtaga aggccacgaa ttgccgcggc gagacagaac agttccgctt gatcaaccag
     1081 tcctttacga ccggttcggt gtccatcggc agatcagttg cttgcatcgc cgtctcccgc
     1141 gtcagatttg tctcacccgc gctgcgtgtt gcttacttgc tgccttatct gctgcttgcc
     1201 tgctgcctgt attgctcgcc tgcgcacact ggattgccag cgccaatcac taggaatgca
     1261 cccaaaatca tgccagcccg gcgacaaact gacgcattat aggcgcgttg cccagcatcc
     1321 acaagcaagg tggtgcgcgc cgcaaagcca agccgcacaa gcctctgcgc ccttttgccg
     1381 catccttttg aatgcttgtc aacgcgtgta ttcgaggggt gggaatggtc tttctgactg
     1441 gtttacttgc tccgttttga gcctcgtcct atatcttcaa gcctcacaat accgtgccct
     1501 tccgccgtca ctcgcggcac tgccttccat gcgtgtccat agatcacttc aaaggtcagc
     1561 gggatcgtgc cgtcttcgcg gcggcgcgct tcaagcgccg cgtgaagcgc gtcacgcaac
     1621 gcgcgagcct gcgtttgcgg cgcaaggtca cgcgggtaag caccccagcg gcgcacgtcc
     1681 ttcaatagcg attccggcga cttgtacgtg atcgtcagca cctcctgatc catcaccgga
     1741 atctcgaaac cgctctcgac cagcatgtca ccgagatcgt gcatatcgac gaattcgata
     1801 gtgtggcgct gcggcgtcag acccagtgat ttttccgcct gcgcgtatgc accgctgagc
     1861 tctttcagtg tgtcgggacc gagcgtgctg aacatcagca ggccgttcac cttgagcaca
     1921 cgtgcccatt cgggaaatac gaggtcgggg cgcgcgtgcc agtgcaacgc gagattcgac
     1981 cagagcatct cgaacgcgct tgcaacaaag ggcagatgag caaaatcgcc ctgcgccagt
     2041 tgcgggccgc gcgcgccgaa tgccttgccc agcgtggcag gtagaaaccg ccgccagccg
     2101 gcgttggctt cggattcagc catctcggcc tgattcgccc gggccagcat ggccgctgac
     2161 aaatccatgc cgagcaccga cgcatcggca aaacgctcgc gcagtccggc caggtcggcg
     2221 cccatgccgc agcccgcgtc gagcacgcgc accggcgtta ccttgatgta gtcgagccgc
     2281 tcgcgcatgc gctgcgcgat ctcgcgcggc aggaacgcaa cgtcatcgaa cgtcgcggcc
     2341 cgacggtcga aaattcgctg cagatgacgc aaatcgtagg ctggacggcc agcattggcg
     2401 ggagttgggg acatgacaac ctactttcta agagtgcgaa gtatactcgg tcgattcctg
     2461 aaacgttcag ccaaaccagg cagcgcaacg gtttgcgccg gttctccggc gcgctcgctg
     2521 ctcatcatcc gctcgcttag ggcagatttt ttgcgctgcc acgctttctt tggatcgaac
     2581 gtatgaagct acggtttggt gtcgtatggc gcgctccagg ctcgctgcgc aaggcttctg
     2641 ccacggcgct gcggcggctc ggccagatcc gcgacatcgc gttgcccagt cagtgcgcat
     2701 tgtgtggcaa tttgtcgcag aaggtgttgt gtgccggctg cgatgaacag tattggaatc
     2761 aggcgcgcac acggtgcccg gtttgtgcat tgcccgtgcc ggttcttgct gcggcctcgg
     2821 ctgcatcagc tggctttaaa aatggtgcct atctgccttg caggcgttgt attgcggagc
     2881 ctccgcattt cgacgcgact attgcgctgg ccgactatca cgcgccgctc gatacactcg
     2941 cgctcgatct caagttccac gcgcggctgg caacggccga cgaatttgcg cgccagttgc
     3001 aagccgcctt cgagagcacc gatgcggcag cacccgatgt cgtcgcacct gtgccgctct
     3061 cgccaaggcg cctgaggacg cgcggttaca accaggcgtg gtcgatcggg aaaccacttg
     3121 cgcgccggct cggctcgttg gctgacgcaa cgctgatcga gcgcacgaga gatacggcac
     3181 cgcaagcaaa gcttgatctc gatacgcggc gcagaaacgt cggcaatgcg ttcagggtgc
     3241 tgcgtgacat acgcggtcag cacgttgcaa tcgtcgacga cgtcatgacc tcgggcgcca
     3301 cgctcgatgc tctggccttt accctgaaag ccgccggcgc tcggcgtgtc accaacttcg
     3361 tcgcgctgcg cacgccgcaa gactaatttt tcacgggttt atcgcttatg ttcaatgtcg
     3421 tactggtcga acctgaaatc ccgccgaaca ccggcaacgt gatccggctg tgcgccaata
     3481 ccggcgcccg cctgcatctg atcgaaccgc tgggtttccc tctcgacgac gccaagatgc
     3541 gtcgcgccgg cctcgattac cacgagtatg cgcagatgca tgtccatgcg aactgggaag
     3601 ctttcctcga aaaggaaatg cccgacgtga gccgcatgtt tgccttcacc acgcgcggct
     3661 caacgccatt tttcgggcat aactttaaag cgggcgactg gtttgtcttt ggtgctgaaa
     3721 cccggggctt gtcggacgcg gtgcttacgg agttcacgaa cgaccggcgc gtgcgtctgc
     3781 ctatgcgacc agggaacagg agtttgaatt tatcgaatac ggtggcaatc gtcacgtttg
     3841 aagcatggcg tcagacaggg ttcgagggcg gtgaatagtc ttcgtgcagt cttcgcgtgc
     3901 ccacgaaccg tcattccgct tttgcgtccc ggctcaacaa cgcgtgaacc gcgtcgcgcg
     3961 gtgcgacgtc ttcgaataac acgcgacaca ccgcgtgtgt aatcggcatc tcgatgccgc
     4021 gagactccgc cagcgccagc acggcacggg cgcatcgcac gccctcggcc acatgaccga
     4081 gcgccgcgag aatgtcggtg agcgaacggc ctgttgccaa ctgtatgccg accgtgcgat
     4141 tgcgcgataa gtcgcccgtc gcggtgagga tcaggtcgcc gagccccgtc aaacccgtga
     4201 aggtttccgc atggccgccg agcgccacgc cgagccgcga catctcggcc agtccgcgcg
     4261 taatcaacgc ggcccgggcg ttcaggccca agccgagtcc atcggcaatg ccggttgcaa
     4321 tggccagcac gtttttcacc gcgccgccta cttccacgcc aatcacgtcg tcgcctgtgt
     4381 aaatgcgcat ggcgccgtga tgaaacgcgg caacggtccg ctcaccaagc gtggccgacg
     4441 cgctcgcgat cgttagcgcc actggcaaac cttgcccgac ttcgcgcgcg aagctcggcc
     4501 cggaaagtgt gccgttgctc gcttgctgcg gcagttcggc cgccaccacc tcgtttggca
     4561 ggcattgcgt gtcggcttca aaacctttgc acaaccagac gatatgcgcc ggaacaatgc
     4621 cgagttcgcg catgttgcgg cataacgcac gcaacccggc taccggagcg gcaaccacgc
     4681 acagagcatc tt
//
LOCUS       DATASH010000042         3604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4950337,
            whole genome shotgun sequence.
ACCESSION   DATASH010000042 DATASH010000000
VERSION     DATASH010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4950337"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VHV99_01410"
     CDS             <1..393
                     /locus_tag="VHV99_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX3634147.1"
                     /translation="RLSETREVMNTIKSYAAEDATVIFGAVYDDAMGDALRVTVVATG
                     LGRAAKKQQSAPMTLLRTGTDNQPVSAHAAYAPSSSHHASTADYGALDAPAVWRQSRD
                     TAASHVQALQEKGVDTYDIPAFLRKQAD"
     gene            641..1144
                     /locus_tag="VHV99_01415"
     CDS             641..1144
                     /locus_tag="VHV99_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX3634148.1"
                     /translation="MIQTGEKLPDATLFEFVEDARAGCTLGPNSFDVREQTAGKRVVI
                     FGLPGAFTPTCSAKHVPGYVEHAAQLRAAGIDEIWCVSVNDAFVMGAWGRDQHASGKV
                     RMMADGSAAFTRALGLEQDLSARGMGIRSQRYAMVVDDGVVKTLNVEAAGKFEVSDAA
                     SILATLS"
     gene            1434..2351
                     /gene="lpxC"
                     /locus_tag="VHV99_01420"
     CDS             1434..2351
                     /gene="lpxC"
                     /locus_tag="VHV99_01420"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEX3634149.1"
                     /translation="MLKQRTIKSIVKTVGIGLHSGRKVDLTLRPAAPDTGIVFSRVDL
                     ATPVDIPASAMAIGDTRLASVLQKDGARVSTIEHLMSACAGLGIDNLYIDVTAEEIPI
                     MDGSAGSFVFLIQSAGIEEQNALKKFIKVTKPVEIRDGDKFARLDPYFGFKLKFTIDF
                     RHPAVDKTGQALEVDFANTSYVREIARARTFGFAHEVEMMRELGLARGGSMDNAIVLD
                     EYRILNNDGLRYDDEFVKHKMLDAIGDLYVIGHPLLASYTAYKSGHGLNNALLRELLA
                     HEDSYELVTFEDTQKAPRGFAYETQTAFA"
     gene            complement(2432..2917)
                     /locus_tag="VHV99_01425"
     CDS             complement(2432..2917)
                     /locus_tag="VHV99_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HEX3634150.1"
                     /translation="MSRIPSFSRLPPKQFKARRPQPVAEVLNRTDAFVALRAGVEQIA
                     ALEKDLRALLPDYLATSVEPSFIKEGVLALFAGHNALAARLRQIEPRLLSDLQQRGWP
                     VNALRIRVRPQPLKEPPPVKQARMTPAGADALHALSESLAPSPLQEALAKMAARHRRN
                     R"
BASE COUNT          706 a         1021 c         1166 g          711 t
ORIGIN      
        1 cgtctgtcgg aaacgcgcga agtgatgaac accatcaaga gctatgctgc ggaagacgcg
       61 accgtgattt tcggcgcggt gtacgacgat gcaatgggcg acgcgctgcg cgtgacggtc
      121 gtggcaacgg gtctgggccg tgcggcgaag aagcaacaat cggcaccgat gacgttgctg
      181 cgcaccggca ccgacaacca gccggtcagc gcgcacgctg cttatgcacc gtcgtcgtcg
      241 catcatgcaa gcacggccga ttacggcgcg ctggatgcgc ccgctgtgtg gcgccagtcg
      301 cgcgatacgg cggcttcgca cgtgcaggcg ttgcaggaaa agggcgtcga cacgtacgac
      361 attccggcat tcctgcgcaa gcaggcggac tgagcgcgtg agcaggcttt cgttgccgcg
      421 ctgtttttag cagcgttggc ggacgaatgc acgagcgtgg cgggtgaacg gcgaacaggc
      481 cgcgtatcgt cagattcgcg gcaaggacga gtgccctctt cggattcgcg aagcggatgg
      541 ggcggttgtc gccgggcgca gcctgttgct gggctgacgc tgaattcact gcgacacgac
      601 gacgtgcgag cgtgcttcgc atcgatgcaa aggactgagc atgattcaaa caggtgaaaa
      661 gctgcccgac gcgacgctct tcgagttcgt cgaagacgcg cgggcgggct gcacgctggg
      721 gcctaacagc ttcgacgtgc gcgagcagac ggcaggcaaa cgcgtggtga tcttcggatt
      781 gccgggcgcg ttcacgccga cctgttcggc caaacatgta ccgggttacg tcgagcatgc
      841 ggcgcagttg cgcgctgccg gcatcgacga gatctggtgc gtgtccgtca acgacgcgtt
      901 cgtaatgggc gcgtggggac gcgatcagca cgcctcgggc aaggtgcgca tgatggcgga
      961 cggcagtgcg gctttcacgc gggcactcgg tcttgagcag gatttgtcgg cgcgcggcat
     1021 gggaattcgt tcccagcgct acgcgatggt tgtcgacgac ggcgtggtca agacgttgaa
     1081 cgtcgaggct gccggcaaat tcgaagtcag cgatgcagcg agtatcctcg cgacgttgag
     1141 ctagttgcgg ttgcgttgaa tgcccatgcc ccgagcctgc gcggtcatcg cgttgtatca
     1201 gggcaacggc gcataagacg cttttgtgac aggccgttac gcgggccgac gaccggaaac
     1261 gcctccgttc cggacatcgg cccgtcacgc ttccctgacg ggcgcccaat gagtaacgca
     1321 gcgaaacaga ttgatacgtt ccgcgctaag acgctcctta gggtattgga gtatacttcg
     1381 cgctatggaa taaaaagtcc cgattgggat tttcaatcga atagaagatc accatgttga
     1441 agcagcgcac tatcaaatca atcgtcaaga cagtcggcat tggcctgcac tctggccgca
     1501 aagtcgacct gacgctccgt ccggcggcgc cggatactgg cattgtgttt tcgcgcgtgg
     1561 atttggccac gccggtggac attcctgcgt cggcaatggc gattggcgat acacgtctgg
     1621 cttctgtgtt gcaaaaagac ggcgcgcgcg tgtcgaccat cgagcatttg atgtccgcat
     1681 gcgcaggcct cggtatcgat aatctttata tcgacgtgac cgctgaagaa attccgatca
     1741 tggacggcag tgcggggtcg ttcgtgtttc tgattcagtc ggccggtatc gaagaacaga
     1801 acgcgctgaa gaaattcatc aaggtcacca agcctgtgga aattcgcgac ggcgataaat
     1861 tcgcgcgtct cgatccgtat ttcggtttca agctcaaatt caccatcgat ttccgtcacc
     1921 ccgccgtgga taagaccggc caggcgctgg aagtggattt tgccaataca tcgtatgtgc
     1981 gggaaatcgc ccgtgcgcga acgttcggct ttgcgcatga agtggaaatg atgcgcgaac
     2041 tcggtttggc acgcggcggc agcatggata acgccatcgt gctcgacgaa taccgcattc
     2101 tgaataacga cggcctgcgt tacgacgacg aattcgtgaa gcacaagatg ctcgacgcga
     2161 tcggcgatct gtacgtgatc ggccatccgt tgctggcatc gtatacggcg tacaagtcgg
     2221 gccacggtct gaacaacgcg ctgctgcgcg agctgctggc gcatgaggat tcgtacgaac
     2281 tcgtcacgtt cgaggacacg cagaaggcgc cgcgcggctt tgcgtacgaa acgcaaacgg
     2341 cgtttgcgtg atctgcgtag cagcttcgga tgaattgtcc gaagcgaatg gatgaaaaat
     2401 aagcggcccc actgggccgc ttattttttg ttcagcgatt tctccgatgc cgcgccgcca
     2461 ttttcgccaa agcctcttgc agcggcgagg gcgcgagcga ttcggagagc gcgtgcaatg
     2521 cgtccgcgcc ggcgggcgtc atgcgcgcct gcttgaccgg cggcggttcc ttcaaaggct
     2581 ggggtcgcac gcgaatcctc aacgcattga ccggccagcc gcgctgctgc agatccgaca
     2641 acaagcgcgg ctcaatctgg cgcaggcgcg cggccagcgc gttgtgcccg gcaaacaggg
     2701 ccagtacgcc ttccttgatg aagctcggct ccacgctggt cgccagatag tcgggcaaca
     2761 aggcgcgcag atccttttcg agcgccgcaa tctgctcgac gcccgcgcgc agggccacga
     2821 acgcgtcggt gcgattgagc acttcggcga cgggctgcgg gcggcgcgct ttgaactgct
     2881 tgggcggcag ccttgaaaag gaaggaatac ggctcatgtt tgatcgtgac ggcgtgttcg
     2941 caccctatgc gcgctcacgc cgcgattgta ccgcgcgggc tgcacgcgga cccgcctcaa
     3001 tgcgtaccgt cagaagtcgc tgcggcgggc ttcctgtgcc gcgccgccgt cgcacggctg
     3061 acacaggcgt gggcgggggc gcgtgctaaa atgcccgatt cggtgcgaca cgcacgctcg
     3121 tgtccggttg ctccacgtta cggagcatcg actagagggt tattgaggta actcaccatg
     3181 ccctccgcag gcggccccct tgtgggccgc actttaggaa gcggatgcaa ccctatggga
     3241 agcatccttg tagtgaaatt ggaaaccgga gcaatcagcc gggaaccttg ttgcatgggc
     3301 ttgttaccgt caggtcccgc ccagtcagcg gcttcacctc cgccgacagg gcggggtgat
     3361 gtttgcaatt cccgagcctt caggcaaggg tttcgctaaa cgatacgagc gaaagctcgg
     3421 tgtgtcattg ttcgacctcg tttgttggaa gacgagggta gtgaacctgc tgtacgacca
     3481 gcagtagcct aggaagacat gcgtcactgg taacggtggg gtgtgaagct cttctgacaa
     3541 tgtagccctc ggatgtttcg ggcagatcga ttccgcgagg agcgatctgg atactcccag
     3601 cagc
//
LOCUS       DATASH010000043         1858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4554927,
            whole genome shotgun sequence.
ACCESSION   DATASH010000043 DATASH010000000
VERSION     DATASH010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4554927"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VHV99_01430"
     CDS             <1..210
                     /locus_tag="VHV99_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004190034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HEX3634151.1"
                     /translation="GANKFRTIIEDDVFVGSDTQLVAPVRVKRGATIAAGTTVWKDVD
                     ADALVLNDKTQTSKTGYVRPTKKKS"
     gene            280..>1858
                     /gene="glmS"
                     /locus_tag="VHV99_01435"
     CDS             280..>1858
                     /gene="glmS"
                     /locus_tag="VHV99_01435"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX3634152.1"
                     /translation="MCGIVGAVAQRNIVPVLIEGLRRLEYRGYDSCGVAVLGDDGPRR
                     ARSVARVADLDEQVRESHLEGITGIAHTRWATHGAPVTDNAHPIFSKDALALVHNGII
                     ENYETLREMLRGKGYTFVSQTDTEVIAHLIHSLYHGDLFAAVREAVGQLHGAYAIAVL
                     HKDQPHTVVGARQGSPLVVGLGQGENFLASDALALAGSTERFIFLEEGDVTEIRRKNI
                     RVIDNEGNTVERPVRESELSADSAEKGPYRHFMLKEIHEQPRAIANTLQERVANGRLL
                     EAAFGPAASEVFRRTQNVHIVACGTSYHAAVTVRYFIEQICKLPCAVDIASEYRYRSP
                     LVPANSLFVTISQSGETADTLAALRLAKESGYLSSLAICNVPESSLVRDSELVMLTRA
                     GPEIGVASTKAFTTQLAALGMLVVALAKHHGADAERERGLVSRLVELPALIEKTLALD
                     PAIYELAKRFADKHHALFLGRGALYPIALEGALKLKEISYIHAEGYPAGELKHGPLAL
                     VDADMPVITVAPNNDLLE"
BASE COUNT          374 a          568 c          592 g          324 t
ORIGIN      
        1 ggcgcgaaca agttccgcac gatcatcgaa gacgacgtgt tcgtcggttc ggacacgcaa
       61 ctggttgcgc cggtgcgtgt gaagcgcggg gcgacgatcg cggcgggcac cacggtctgg
      121 aaagacgtcg acgccgacgc gctggtcctg aacgacaaga ctcaaacgag caaaacgggc
      181 tacgtgcgcc cgaccaaaaa gaagagctga taggcgtgag gcgcgtaaaa aacgcgctca
      241 ttgaaatccg gcacttagag cactgaaaag gaatgagcca tgtgtggcat tgtcggcgcg
      301 gtagcgcaac gtaacatcgt tcccgtcctg atcgaaggac tgcgtcgcct cgagtatcgc
      361 ggctacgatt cgtgcggcgt ggccgtactc ggcgacgacg gtccgcgccg cgcgcgcagc
      421 gtggcgcgtg tggccgatct cgacgagcag gtgcgcgaga gccatcttga aggcatcacg
      481 ggtatcgcgc atacgcgctg ggcgacgcac ggcgcgccgg tgacggacaa cgcgcacccg
      541 atcttctcca aagatgccct cgcattggta cacaacggca tcatcgagaa ttacgaaaca
      601 ctgcgcgaga tgctgcgcgg caagggctac acgtttgtct cgcagaccga cacagaggtt
      661 atcgcgcatc tgattcacag cctgtaccac ggcgatctgt tcgcggcggt gcgtgaagcc
      721 gtcgggcaat tgcacggcgc gtatgcgatc gccgtgttgc ataaggacca gccgcatacg
      781 gtggttggcg cgcgccaggg ttcgccgcta gtggtcgggc tcggccaggg tgagaacttc
      841 ctcgcctcgg acgcgctggc gctcgcgggc agcaccgaac gcttcatctt cctggaagag
      901 ggcgacgtga ccgagatccg ccgcaagaac atccgggtga tcgacaacga aggcaacacg
      961 gtcgagcgcc cggtacgcga gagcgagctg tctgccgatt ccgcggagaa gggaccctac
     1021 cggcatttca tgctgaagga gattcacgag cagccgcgtg cgattgcaaa cacactgcag
     1081 gagcgggtgg ccaacgggcg tttgctggag gcggccttcg ggcccgccgc cagtgaagtt
     1141 ttcagacgga cccagaacgt acacatcgtg gcttgcggca ccagttatca cgccgcagtg
     1201 accgtccgtt atttcatcga gcagatctgc aaactgccct gcgcggtaga tattgcgagt
     1261 gagtaccgct accgcagtcc gctggtaccg gccaattcgc tgttcgtgac gatctcccag
     1321 tcaggggaaa ccgccgacac cctcgccgcc ctgcggctgg cgaaagaatc cggttacctg
     1381 tcatcgctcg cgatctgcaa cgtaccggaa agctccctgg tgcgcgactc ggaactcgtg
     1441 atgctcacgc gggcgggtcc cgagatcgga gtcgcttcaa ccaaggcttt caccacacag
     1501 ctcgccgctc tcggcatgct ggtagtggca ctggcgaagc atcacggtgc ggatgccgag
     1561 cgcgaacgcg gcctcgtctc gcggctggtc gagttgcccg cgctcatcga gaagacgctg
     1621 gcgctggatc ctgccatcta tgagctggcc aagcgctttg ccgacaagca tcacgccctg
     1681 ttcctgggac gcggtgcgct gtacccgata gccctcgaag gtgcactgaa gctcaaggaa
     1741 atttcctaca ttcacgctga aggttatccg gccggtgagc tgaagcacgg cccgctggcc
     1801 ctcgtggatg ccgatatgcc cgtgattacc gtcgcgccca ataacgatct gctcgaga
//
LOCUS       DATASH010000044         4668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5741835,
            whole genome shotgun sequence.
ACCESSION   DATASH010000044 DATASH010000000
VERSION     DATASH010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4668
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5741835"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VHV99_01440"
     CDS             complement(<1..257)
                     /locus_tag="VHV99_01440"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase"
                     /protein_id="HEX3634153.1"
                     /translation="MNKPDDIKTDTPAVQKDEAEWRNQLSDIEYQVTRNAATERPFTG
                     RYHDHWDRGIYDCVCCGTPLFESDTKFDAGCGWPSYFKPING"
     gene            complement(383..1939)
                     /locus_tag="VHV99_01445"
     CDS             complement(383..1939)
                     /locus_tag="VHV99_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HEX3634154.1"
                     /translation="MSFSPSIAGLSRPLSALSDALVTSPESAFARLGSAFLTRLPAAP
                     LSAPYLVGFSEETAALLGLEPGLQSDPGFTELFSGNTTREWPSTALPYASVYSGHQFG
                     VWAGQLGDGRALGLGEIEHHGQRFELQLKGAGRTPYSRMGDGRAVLRSSIREYLCSEA
                     MHHLGIPTTRALCVIGSDQPVRREEVETAAVVTRVAPSFVRFGHFEHFYSNDRVDALR
                     ALADHVIERFYPHCREADDPYLALLDEAVRSTADLMVEWQAVGFCHGVMNTDNMSILG
                     LTIDYGPFGFMDGFDAGYICNHSDSQGRYAYKMQPQIAYWNLFCLAQGLLPLLGVQHE
                     ESVRGEKSIEDAQRVLGGFKDRFAPALENRMRAKLGLETGRDGDDSLINRLFEVMHAN
                     RADFTLTFRNLARISKHDASGDAPVRDLFLDRAAFDAWANDYRARLSEETRDDAARAI
                     AMNRVNPKFVLRNHLAETAIRRAKEKDFSEVERLAAVLRRPFDEQPEHEAYAGLPPDW
                     ASSLEVSCSS"
     gene            2176..3861
                     /locus_tag="VHV99_01450"
     CDS             2176..3861
                     /locus_tag="VHV99_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(methylthio)propionyl-CoA ligase"
                     /protein_id="HEX3634155.1"
                     /translation="MTTPLFGQMMDVPLTVSSLLAHAARHFGDTGIVSRRIEGDVHRY
                     TYRDCEKRAKQLAQALIALGVEPGERVATLAWNGYRHLEAYYGITGFGAVCHTINPRL
                     FPDQIAYIVNHADDAYVLFDTTFAPLVDCLAPQCPKVRGWIALADEAHVPKMQSPALS
                     YETLLAAQDGHYEWPQLDERQASYLCYTSGTTGNPKGALYSHRSTVLHAFGAALPDAM
                     SLSARDAVLPVVPMFHVNAWGIPHSAPLTGAKLVFPGKDLDGKSLYELMESERVTYSA
                     GVPTVWLGLLNYLREAKVRFSSLNRTVIGGSACPPAMLRTFEDEYGVQVIHAWGMTEM
                     SPLGTLSKLTWEQSQRPLAERRKLLEKQGHVLYGVDMKIVGEDGRDLPWDGVAFGDLH
                     VRGPWVIDRYFRKDDSPLVDGWFPTGDVATIDRDSFLHITDRSKDVIKSGGEWISSID
                     IENVAIAHPAVAEAACIACAHPKWTERPLLVVVKRAGFDVTREELLAFYDGKVAKWWI
                     PDDVAFVDELPHTSTGKLQKLKLRDTFRDHILPSALADEKDCPLTPLARGNPV"
     gene            4016..>4668
                     /locus_tag="VHV99_01455"
     CDS             4016..>4668
                     /locus_tag="VHV99_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX3634156.1"
                     /translation="MAVDYTTHDGVAVITLNNPPVNGLGLSTRAGIVEGIERAQSDPA
                     IKAIVLTGAGKAFSGGADITEFNTPKATQEPTLATVIKTVEGSAKPVVAAIHSVAMGG
                     GLELALGAHYRIAAPAAQIALPEVKLGILPGAGGTQRLPRAIGLEAALNMIVSGAPVM
                     SEKLADSGLLDEVAQGDLAEAALAFARKVGAKAGPHPKVRDRKIEHPNAAGFIQFAR"
BASE COUNT          854 a         1471 c         1506 g          837 t
ORIGIN      
        1 ccgttgatcg gcttgaagta gctcggccag ccgcaaccgg cgtcgaactt ggtatccgat
       61 tcgaacagtg gcgtgccgca gcacacgcag tcatagatgc cgcggtccca gtggtcgtga
      121 tagcggccgg tgaaggggcg ctcggtggcc gcgttgcggg tcacctggta ttcgatgtcg
      181 gagagctggt tgcgccattc ggcctcgtct ttctgcacgg cgggggtgtc ggtcttgatg
      241 tcgtcaggtt tgttcatgat tggggttcct gtctcgggct tgtttttcgg ggctgcaaag
      301 tgttcaggca gattcggctc actaaacgga ttcagcggat tcaagcggat tcaaagctga
      361 ttcaagcgag ttcggctcgg cgtcacgaag agcaactcac ttccagcgag ctggcccaat
      421 ccggcggcag ccccgcatac gcttcgtgtt ccggctgttc gtcgaatggg cggcgcagca
      481 cggccgccaa acgctccact tcggagaaat ccttctcttt cgcgcggcgg atcgccgttt
      541 ccgccagatg attgcgaagt acgaatttgg ggttcacgcg gttcattgca atggcgcgcg
      601 cggcgtcgtc gcgtgtctct tcggacaggc gcgcgcggta gtcgttcgcc cacgcatcga
      661 acgccgcgcg gtcgaggaac aggtcgcgca ccggcgcgtc gccgctcgcg tcgtgcttcg
      721 aaatacgcgc cagattgcgg aacgtcagcg tgaaatccgc acggtttgcg tgcatcactt
      781 cgaacaggcg gttgatcaga ctatcgtcgc catcgcgccc ggtttcgagg ccaagtttgg
      841 cgcgcatgcg gttttccagg gccggcgcga aacggtcctt gaagccgccg agcacacgct
      901 gcgcgtcttc gattgacttc tcgccgcgca cgctttcctc atgctgaacg cccagcagcg
      961 gcagcaggcc ctgtgcaaga cagaagaggt tccagtacgc gatctgcggc tgcatcttat
     1021 acgcgtaacg gccttgcgag tcggagtgat tgcagatgta gccggcgtcg aaaccatcca
     1081 tgaagccgaa cgggccgtag tcgatcgtga gaccgaggat cgacatgttg tcggtattca
     1141 tcacgccgtg gcagaaaccg acagcttgcc attcgaccat cagatcggct gtcgacctca
     1201 ccgcctcatc gagcagcgca agatacggat cgtcggcttc gcggcagtgc ggatagaagc
     1261 gttcgatcac atgatcggcg agcgcgcgca gtgcatcgac gcgatcgttc gaatagaaat
     1321 gctcgaagtg cccgaagcgc acgaagctcg gcgccacgcg cgtgacaacc gccgcggttt
     1381 cgacttcctc gcggcgcacc ggctggtcgg agccgatcac gcacagcgcg cgcgtggtcg
     1441 gaatgccgag gtgatgcatc gcttccgaac acagatactc gcggatcgac gaacgcaaca
     1501 ccgcgcggcc gtcacccatg cgcgaatagg gcgtgcggcc cgcgcctttg agttgcagct
     1561 cgaagcgttg gccgtggtgt tcgatttcgc ccagaccgag cgcgcggccg tcgccaagct
     1621 ggccggccca cacgccgaac tgatgccccg aatacaccga tgcgtacggc agcgcggtgg
     1681 aaggccactc gcgcgttgtg ttgccggaga agagttcggt gaagcccgga tcgctctgga
     1741 ggcccggctc caaaccgagc aacgcagcgg tttcttcgga gaatccgacc aggtagggcg
     1801 cactgagcgg cgccgcgggc aggcgcgtca gaaacgcgct gccgagtcgc gcaaacgcgc
     1861 tttcgggcga ggtaacgagt gcgtcggaga gagccgataa aggcctggat aaccctgcaa
     1921 tgcttgggga aaacgacatg ttgagcgcct ctggattaac cgatattgta attcggcgcc
     1981 cgcggcgctg ctggccggcc cttaggccga cgccgcctgt gtcccgggcg tctcccgcca
     2041 gcgtgcgcct gcgccgcttc aaaacgcgcc acgcgagcca ccggaacgtc ccctgcgacc
     2101 aaacggtgcg cgccgcacgg ccccgagctg caccccaatg acgcacacaa ggcgaggaga
     2161 gttctcttta tgacgatgac gacgccgctg tttggccaga tgatggacgt gccgctcact
     2221 gtgtcctcgt tgctcgcgca tgccgcgcgg catttcggcg acaccgggat cgtgtcgcgg
     2281 cgcatcgaag gcgacgtgca tcgctacacc taccgcgact gcgaaaagcg cgcgaagcag
     2341 ctggcgcagg cgctcatcgc gctcggcgtg gagcccggcg agcgggtggc cacgctggcc
     2401 tggaacggct accgccatct ggaagcgtat tacggcatca ccggcttcgg cgcggtgtgt
     2461 cacacgatca atccgcgtct ttttcccgac cagatcgcct acatcgtcaa tcacgccgac
     2521 gacgcctacg tgctgttcga caccacgttc gcgccgctcg tcgattgtct cgcgccacag
     2581 tgtccgaagg tgcgcggctg gatcgcactg gccgacgaag cccacgtgcc gaagatgcaa
     2641 tcaccggcac tgagctatga aacgttgctg gccgcacagg acggccatta cgaatggccg
     2701 cagctcgacg agcggcaggc gtcgtacctt tgctatacgt ccggcaccac cggcaatccg
     2761 aagggcgcgc tgtattcgca tcgctcgacg gtgctgcatg cgttcggcgc agcactgccc
     2821 gatgccatga gcctgtccgc gcgcgacgcc gtgctgccag tcgtgccgat gtttcatgtg
     2881 aacgcctggg gcattccgca ctccgcgccg ctgaccggtg cgaagctggt ctttcccggc
     2941 aaggatctcg acggcaaatc gctgtatgaa ctgatggaaa gcgaacgcgt cacgtattcg
     3001 gccggcgtgc cgaccgtgtg gctcggcttg ctcaactatc tgcgcgaggc caaggtgcgt
     3061 ttctcttcgc tcaatcgcac ggtgatcggc ggctcagctt gtccgccggc catgctgcgc
     3121 accttcgaag atgagtacgg cgtgcaggtg atccatgcat ggggtatgac cgaaatgtca
     3181 ccgctcggta ccttgtcgaa actgacgtgg gaacagagtc agcgtccgtt ggcggagcgg
     3241 cgcaaactgc tcgagaagca gggccatgtg ctgtatggcg tcgatatgaa gatcgtcggc
     3301 gaagacggcc gcgatctacc gtgggacggt gtcgcgttcg gcgatctgca cgtgcgcggg
     3361 ccgtgggtga tcgaccggta tttccgcaag gacgactcgc cgctcgtgga cggctggttt
     3421 ccgaccggcg acgtcgccac catcgaccgt gacagctttc tgcacatcac ggatcgctcg
     3481 aaagacgtga tcaagtcggg cggcgagtgg atcagctcga tcgacatcga gaatgtcgcc
     3541 atcgcgcacc ccgcggtggc cgaagccgcg tgcattgcct gcgcgcaccc gaagtggacc
     3601 gagcggccgc tgctggtggt cgtcaaacga gccggcttcg acgtgacgcg cgaagaactg
     3661 ctcgcgttct acgacggcaa ggtcgccaaa tggtggattc ccgacgacgt cgccttcgtc
     3721 gacgaactgc cgcacacgtc caccggcaag ctgcagaaac tcaagctgcg cgacaccttc
     3781 cgcgaccata tcctgccgtc cgcgctcgca gacgaaaagg actgcccact cacgccgctc
     3841 gccaggggaa accccgtcta aggataaatt gaacgagcgt gcttttttgt gtattctgcc
     3901 tgctgacccc gggaaagtcg cagaatgggt cgactcaaga gtcgatctct ctgcggctcg
     3961 gtgaactggg acgggcggcg cgtcatgcgc cgtctcatcg catggaggca ggcagatggc
     4021 agtggactac acaactcatg acggcgtggc cgtcatcacg ctgaacaatc cccccgtaaa
     4081 cggcctcggc ctctcgacgc gagccggtat cgtcgaaggg atcgagcgcg cgcagagcga
     4141 tccggccatc aaggcaatcg tgctgacagg cgcgggcaaa gccttctcgg gcggcgccga
     4201 catcaccgaa ttcaacacgc cgaaggcgac ccaggagccg acgctcgcca cggtcatcaa
     4261 gaccgtcgag ggcagcgcga agccggtggt tgccgcgatt cacagcgtcg caatgggcgg
     4321 cgggctggag ttggcgctcg gcgcgcatta ccgcatcgcc gcacccgccg cgcagatcgc
     4381 gctgccggaa gtgaagctcg gcattctgcc gggcgcgggc ggcacgcagc gtctgccgcg
     4441 tgccatcggc ctcgaagccg cgctcaacat gatcgtctcc ggtgcgccgg tgatgtcgga
     4501 gaaattggcc gactcgggtc tgttagacga agtggcgcaa ggcgatctgg ccgaagcggc
     4561 gctcgcgttt gcccgcaagg tcggcgccaa agcgggcccg catccgaagg tgcgcgaccg
     4621 caagatcgag catccgaatg cggctggctt catccagttc gcgcgcaa
//
LOCUS       DATASH010000045         7863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_202785,
            whole genome shotgun sequence.
ACCESSION   DATASH010000045 DATASH010000000
VERSION     DATASH010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7863
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202785"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VHV99_01460"
     CDS             <1..369
                     /locus_tag="VHV99_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX3634157.1"
                     /translation="KIDAIVASNDGTAGGAIQALAAQHMAGKVPVSGQDADLAAVKRV
                     IAGTQTMTVYKPLKLIAGEAAKLSVALAKGEKPAFNAQYDNGKKKVDTVLLQPTLLTK
                     SNVDVVVKDGFYTQAQLASQ"
     gene            458..2026
                     /gene="xylG"
                     /locus_tag="VHV99_01465"
     CDS             458..2026
                     /gene="xylG"
                     /locus_tag="VHV99_01465"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HEX3634158.1"
                     /translation="MTQPLLTMRGIVKTFSGVKALDGIDLTVAPGECVGLCGENGAGK
                     STLMKVLSGVYPHGTWDGEIIWEGGPLKAASIRDTERAGIIIIHQELMLVPELSVAEN
                     IFLGNEITLPGGRMNYAAMYQRADELLRELGISGINSAQPVMNYGGGHQQLIEIAKAL
                     NKRAKLLILDEPSSSLTTTEIHILLDIVRDLKRRGVACVYISHKLDEVAAVCDTISVI
                     RDGRHVATEPMRALTTDRIISLMVGREIKNLFPREPHPIGDVIFEARHVTCFDVTNPR
                     RKRVNDVSFALRRGEILGVAGLVGAGRTELMQAIFGAYPGVSEATVVMEGKPLKIRAP
                     VDAIRAGIGMVPEDRKRHGTVPGLSVGHNITLAVLQRFAKGGRIDSAAELDTIHTEMK
                     RLSVRAAHPMLSIASLSGGNQQKAVLTRMLLTDPKVLILDEPTRGVDVGAKYEIYKLI
                     FQLAQRGMAIVMVSSELPEVLGISDRVLVIGEGELRGDFVNDGLTQEDILSAAIRPVQ
                     RSLNPSAPTEASAA"
     gene            2023..3222
                     /locus_tag="VHV99_01470"
     CDS             2023..3222
                     /locus_tag="VHV99_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX3634159.1"
                     /translation="MTPDVTSQSAEGAVPRGAFGGPQRIQQLFARYKILALLIAVAAI
                     WIFFSFLTHGAFVTPRNLSNLLRQMSITGMLACGMVFVIIAGEIDLSVGSLLGLLGGV
                     AAILDVNRHWPIGVTVPVVMLLGVLVGMFNGWWSTYRRVPSFIVGLGGMLAYRGILLG
                     VTGGSTIAPVSDGFVFVGQGYLPRLAGDSLAVVLFVVLAFLTLRQRRNRERYKLRVVP
                     VWQDVMKVVGAGVILAAFVATLDRYGGIPVPVLLLLALLGIFTWIATQTVFGRRIYAV
                     GSNLEATRLSGVNTNRVKLAIFALMGLMCAFAGIVNTARLAAGSPSAGSMGELDAIAA
                     CFIGGTSMRGGSGTVYGALIGALVMASLDNGMSMLDVDAYWQMIVKGSILVLAVWIDV
                     VSGSNRR"
     gene            complement(3252..4190)
                     /locus_tag="VHV99_01475"
     CDS             complement(3252..4190)
                     /locus_tag="VHV99_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017335564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX3634160.1"
                     /translation="MHMPLDSSLLGGLRCFEVAARLLSFTKAAAELSLTQGAVSQHIR
                     HLEDRLGYALFTRKHRALALTQKGATLFECTRNAFLSIETTINQLARSNAPLQINCSP
                     SFALQWLMPRLNEFHRLHPDIPVRVKAEFQTLDRQTMLADDIDVAIRFDPGQYETIRA
                     IPILDEYLLPVATPQYLAEHPEFAAGQSLKGIELLHDFSPWDGAPEFIEWRTWLEAVR
                     PEWLGAVAGPQFNLASLAISAALSHQGVAVARTALVYEELEHGRLVNIFGRLVPAPAR
                     YMLLTERQENARVAAFSNWLMDECHRFSDARAKLLL"
     gene            4288..5625
                     /locus_tag="VHV99_01480"
     CDS             4288..5625
                     /locus_tag="VHV99_01480"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HEX3634161.1"
                     /translation="MSPTLSAFAPDLLARLQKRTPLLWLNPALGSALPEGSPCAADID
                     EADARLVRCAGLMAHLFPELQSSAGLIESPLMRVERLQQTMGNATEADAAWFIKSDHA
                     LPIAGSVKARGGFHEVLAFAEKLALEHNVLAHDGDRRELAGDAARALFAQYTVSVGST
                     GNLGLSIGVMAAALGFQSVVHMSTDAKAWKKDRLRKCGVQVVEHAGDYAEAVAAGRAQ
                     SLADPKGYFVDDENSLMLFLGYAVAARRLATQLAALNRPVNATHPLFVYIPCGVGGAP
                     GGITFGLKALFFEHVHCFFAEPAASPCMLVQLAAGETAPVSVYDIGLDNRTEADGLAV
                     GQASHLVSPLMRSQLSGVFTVADAQLYVHLHRVAQTEGVLLEPSAAAGFDGPRWLTHS
                     DQGQAYLRQHGLERHMASATHIVWTTGGSLVPREEHEQFQARGQVLAAQDELA"
     gene            5712..7034
                     /locus_tag="VHV99_01485"
     CDS             5712..7034
                     /locus_tag="VHV99_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3634162.1"
                     /translation="MIETPIDTSTIVDEAAHTSTFQEATYRKVALRFIPFLMVCYVVA
                     FLDRINIGIAKLNMLTDLRFSETAYGLGAGLFFLGYLIFEVPSNLLMHRIGAKLWIAR
                     IMITWGLLSGLMVFVTTQWQFYTVRLLLGVAEAGFYPSVILYLTYWFPNHRRAKMTAC
                     FQAGIPIAGLIGNPLSGWLMDTFHHVAGHSGWQWMFGLEALPTIPLAIGALLILDNSV
                     SAARWLSPQEKQLIVHELGKDEKTREHLSLLKVFTDGRIWKIILTGFPAMMGLYVLGF
                     YLPTLIKASGVTTSTSVGFLSAIPYLVAVIAMVTFGRSSDRMRERRWHLAIVLLLGAA
                     GLTASVWTSHSVVLTVITMSIAAAGIISYSPIMWSVPTAFLGGAAAAASIGVINSAIN
                     IGGFVSPYLIGWVKDEFHTTAPAIYIVAGCLTFGALMALSFDRKLVNR"
     gene            7071..>7863
                     /locus_tag="VHV99_01490"
     CDS             7071..>7863
                     /locus_tag="VHV99_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HEX3634163.1"
                     /translation="MSTTDKPMRFVMGGIIHETNTYAADVAGQTPLASFEQYYGDAIP
                     GIFTGSNHQVGGYIEGARKHGARLIHTFLGQATPSGTIEGNAYAHMKQAILAGIEQAL
                     PVDGVLLALHGAGVADGIEDIEGDLAAAVRTLVGPHVPIAAAYDLHGNMTGQMQHAVD
                     ISLPCILYPHTDFYDRGIECVDLMVKMIRGEVKPITHVCTLPMLPYIVTTNEGFVPAK
                     VNELCARLEALPGVLDCSWFHGFPYADIASPCPAAICTTNGDPALA"
BASE COUNT         1473 a         2315 c         2431 g         1644 t
ORIGIN      
        1 aagatcgatg caatcgtcgc ttcaaacgac ggcaccgccg gtggcgcgat ccaggcgctc
       61 gccgcgcagc atatggcggg caaggtgccg gtgtcgggcc aggacgcaga tctggccgca
      121 gtgaaacgtg tgatcgccgg cacgcagacg atgaccgtgt acaagccgct gaaactgatc
      181 gccggcgaag cggcaaagct ctcggtcgcg cttgcgaagg gcgagaagcc ggcctttaat
      241 gcccagtacg acaacggcaa gaagaaggtc gacacagtgc ttctgcaacc tacattactc
      301 actaagagca atgtcgacgt ggtcgtcaag gacggcttct acacccaggc ccagctcgcg
      361 agccaatgag cgtaacgtgt gtgcggtaaa agccggtgtg cctgatacgc gtcaggcacg
      421 tcggcgtcac cgcgcgcctt gcagcgaggc atagcgaatg acgcaaccac tactgacgat
      481 gcgcggcatc gtcaagacgt tttccggcgt gaaggcgctg gacggcatcg acctgacggt
      541 ggccccaggc gagtgcgtgg gcttgtgcgg agaaaacggc gcaggcaaat cgacgctgat
      601 gaaagtgctg tccggcgtct atccacacgg tacctgggac ggcgaaatca tctgggaagg
      661 cggaccgctg aaggccgcga gcataaggga tacggaacgc gcgggcatca tcatcatcca
      721 ccaggagttg atgctggtgc ccgagctctc ggttgcggag aatatctttc tcggcaatga
      781 aatcacgctc cccggcggca gaatgaatta cgccgcgatg tatcagcgcg ccgatgaact
      841 gctgcgcgaa ctcggcataa gcggcatcaa ctccgcgcag ccggtgatga attacggcgg
      901 cggtcaccag caattgatcg agatcgcgaa ggcgctgaac aagcgcgcga aactgttgat
      961 cctcgatgaa ccgtcgtcgt cgttgaccac tactgagatc catattctgc tggatatcgt
     1021 gcgtgatctg aagcgacgcg gtgtcgcctg cgtgtatatc tcgcacaagc tcgacgaagt
     1081 agcggccgtg tgcgacacga tcagcgtgat tcgcgacggc cgccatgtcg ctaccgagcc
     1141 gatgcgtgcc ttgaccacgg accgcatcat ttccttgatg gtgggccgcg agatcaagaa
     1201 cctgtttccg cgcgaacctc atcctatcgg cgacgtgatc ttcgaagcgc gccacgtcac
     1261 ctgtttcgat gtcactaatc cacgccgcaa gcgggtcaac gacgtgtcgt ttgcgctgcg
     1321 gcgcggcgag attctcggcg ttgcggggtt ggttggcgca gggcgcacgg agctgatgca
     1381 ggcgatcttc ggcgcgtatc ccggtgtgag cgaagcgacg gtggtgatgg aaggcaagcc
     1441 attgaagatc cgtgcgcccg tggatgcgat tcgcgccggt atcggtatgg tgccggaaga
     1501 tcgcaagcgc cacggcaccg tgccgggtct gagcgtcggt cacaacatca cgcttgctgt
     1561 gttgcaacgc ttcgcgaagg gtggccggat cgattcggcc gccgaactcg acacgattca
     1621 cacggagatg aagcggcttt cagtgcgcgc ggcgcatccg atgttgtcga tcgcgagctt
     1681 gtcgggtggc aatcagcaga aggctgtgct cacgcgcatg ctgttgaccg accccaaagt
     1741 gctgattctc gacgaaccga ctcgcggtgt cgacgtcggt gcgaaatacg aaatctacaa
     1801 actgattttt caactggccc agcgcggtat ggcgatcgtg atggtgtcgt ccgaattgcc
     1861 cgaagtactg ggcatcagcg atcgcgtgct ggtgatcggc gaaggcgaat tgcgcggcga
     1921 tttcgtcaac gacggcctca cgcaagagga cattctcagc gccgcgatcc gtcctgtgca
     1981 gcgatccctg aacccgagcg caccaaccga agcgagtgcc gcatgactcc cgacgtcact
     2041 tcccaatccg cagaaggcgc tgtgccgcga ggcgcattcg gtggcccgca gcgcattcag
     2101 caattattcg cgcgctacaa gattctcgcg ttgctgatcg ccgtggccgc gatctggatt
     2161 ttcttttctt ttctgacgca cggtgcgttt gtcacgccgc gcaatctctc gaatctgctg
     2221 cggcagatgt cgattaccgg catgctcgcg tgtggcatgg tgttcgtgat catcgcaggc
     2281 gagatcgatc tgtcggtcgg atcgttgctt ggtttgctcg gcggcgtggc ggcgattctc
     2341 gacgtgaacc ggcattggcc gatcggcgtc accgtgccgg tggtgatgct gctcggcgta
     2401 cttgtcggca tgttcaacgg atggtggtcg acgtatcggc gagtgccttc gttcattgtc
     2461 ggcctggggg gcatgctcgc gtatcgcggc attctgctcg gcgtcacggg cggttcgaca
     2521 attgcgccgg tttccgacgg ctttgtgttc gtcggccaag ggtatttgcc gcgcctcgcg
     2581 ggcgactcgc tcgctgtggt gctgtttgtg gtgctggcgt ttctgacgct gcgccagcgg
     2641 cgcaaccgcg agcgttataa gttgcgcgtg gtgccggtat ggcaggacgt catgaaggtt
     2701 gtcggtgccg gcgtgattct ggccgcgttt gtcgccacgc tcgatcgtta tggcgggatt
     2761 cccgtgccgg ttctgctgct gctcgcgctg ctgggtattt tcacgtggat cgccacccag
     2821 accgtattcg ggcggcgcat ctatgcggtg ggttccaacc tggaagcgac gcggctctcc
     2881 ggtgtgaata ccaatcgcgt gaagctcgct atcttcgcgt tgatgggact gatgtgcgcg
     2941 ttcgccggga tcgtcaatac ggcgcggctc gcggccggtt cgccgtcggc gggatcgatg
     3001 ggcgaactcg atgcgatcgc tgcgtgcttt atcggcggga cctcgatgcg tggcggttcc
     3061 ggaacggtct acggtgcgtt gatcggcgca ctggtgatgg cgagtctgga caacggcatg
     3121 tcgatgctcg acgtcgacgc ttactggcag atgatcgtga agggcagcat tctggtgctg
     3181 gctgtgtgga tcgatgtggt gtcagggtcg aaccggcggt gaagcgtcca ccggcaacgc
     3241 gcgtcggcca ttcacagcag cagcttggcc cgtgcgtcgc taaagcggtg gcattcatcc
     3301 atcagccagt tcgaaaatgc cgccacacgc gcattctctt gccgctcagt gagtagcatg
     3361 tagcgggccg gcgcgggcac gaggcggcca aagatgttga cgaggcggcc gtgctcgagc
     3421 tcttcgtaga cgagcgcagt gcgcgccacg gcgacgccct gatgactcag ggccgcactg
     3481 atcgccaggc tcgccagatt gaattgcggc cccgccaccg ctccgagcca ttcgggccga
     3541 acggcctcca gccaggtgcg ccattcgatg aactcaggcg ctccatccca tggcgaaaag
     3601 tcatgcaata gttcgatccc cttgagcgat tgacccgcgg caaactcagg gtgctcggcc
     3661 aaatattgcg gtgttgccac cggcagcaga tattcgtcga gtatcggtat cgcgcggatc
     3721 gtctcgtatt gcccgggatc aaagcgaatc gctacgtcga tgtcatcggc caacatggtc
     3781 tgtcgatcca gcgtttggaa ttcagccttc acgcgcaccg gtatgtccgg atgcaggcga
     3841 tgaaactcat tcagacgcgg catcaaccac tgtaaggcaa atgatggcga gcaattgatc
     3901 tgtaagggcg cattggatcg ggcaagctga ttgatggttg tctcgatgct gagaaacgcg
     3961 tttctcgtgc attcgaacaa cgttgcacct ttctgcgtca gggcaagggc gcggtgcttg
     4021 cgcgtaaaca gagcgtaacc cagccggtct tcgagatgcc gaatgtgctg actaaccgcc
     4081 ccctgcgtca ggctcagctc agctgccgcc ttggtaaaac tcaacaggcg agccgccact
     4141 tcaaagcagc gcaggccgcc cagcagtgag gaatcgaggg gcatatgtat tagcaggact
     4201 aatgcgtcgg atagaaatta tagctttttt gcctgacgcc cctgacctat attggcgcat
     4261 ccaaaacacc aagcataggt aaccggcgtg agtcccaccc tttccgcatt tgcccccgat
     4321 ctattagccc ggctacagaa gcgtacgccc ctactgtggc tcaatcccgc actcggaagt
     4381 gcgctgcccg agggctcacc gtgcgccgcc gacatcgacg aggcagacgc gcgcctggta
     4441 cgttgcgcgg gcttgatggc tcatcttttt cccgaactgc aaagtagcgc gggcctgatt
     4501 gaatcgccgc tcatgcgtgt cgagcgactc cagcagacca tggggaacgc tacggaggct
     4561 gacgctgcat ggttcatcaa aagtgaccat gccctgccca ttgccgggtc ggttaaagcg
     4621 cgcggaggct ttcacgaagt actggcattc gcggaaaagc tggctctcga acacaacgtg
     4681 ctggcccatg atggcgaccg gcgggaactc gcgggcgacg cagcccgcgc attgtttgct
     4741 caatacaccg tgagcgtcgg cagcaccggc aatctgggct tgagcatcgg cgtcatggct
     4801 gccgccctgg gatttcagtc agtggtacat atgtcgacgg acgccaaagc ctggaaaaaa
     4861 gatcgcttga gaaaatgtgg cgttcaggtg gtcgagcacg cgggcgacta cgcggaagcg
     4921 gtggcggccg ggcgagcgca atcgctggct gatccaaaag gctatttcgt cgacgacgag
     4981 aattcgctga tgctttttct gggctatgca gtcgccgcgc ggcggctagc gacgcaattg
     5041 gccgcgttga atcgccctgt caatgccacg catcctctgt ttgtttacat tccctgtggt
     5101 gtcggaggcg cgcccggcgg cattaccttc ggactcaaag cgcttttttt tgagcatgta
     5161 cactgctttt tcgccgaacc cgcagcgtcg ccgtgcatgt tggtgcaatt ggcggcgggc
     5221 gagactgcgc ccgtgtcggt ctatgacatt ggactggaca atcgcaccga agcggatggc
     5281 ctcgccgtcg gtcaggcctc gcatctggtg agcccgctga tgcgatctca gctctcgggc
     5341 gtgttcaccg tcgcggacgc gcaactgtat gttcatctcc atcgtgtggc gcaaaccgaa
     5401 ggcgtgctgc tcgagccatc ggctgccgcc ggattcgacg gaccgcgatg gctgacgcac
     5461 agcgaccaag gtcaagccta tcttcgccag cacgggctgg agcgtcacat ggctagcgcc
     5521 acccatatcg tctggacgac aggcggttcg ctagtgccgc gtgaagaaca cgagcaattt
     5581 caagcgcgcg gccaggtgct tgcggcgcag gatgaactcg cctgaaaccc ctgcagggca
     5641 ttgccgccaa ccgtccatca cagcatccag cgttagcgca ccgagatgca ctgccaagga
     5701 aacccagttc catgattgaa accccaatcg acacgtcgac catcgtcgac gaggccgccc
     5761 acacgagcac ctttcaggag gcgacttacc gcaaggtagc gctcaggttt attcccttcc
     5821 tgatggtgtg ctatgtggtc gcctttctgg atcgcatcaa tatcggcatt gccaagctga
     5881 atatgctgac tgatctacgg ttcagcgaga cggcctacgg tcttggcgca gggctctttt
     5941 tccttggata tctgattttc gaagtgccga gcaatctgct catgcatcgt atcggcgcga
     6001 agctctggat cgcgcgaatc atgatcacct ggggcctgct ttcggggctg atggttttcg
     6061 tgaccaccca gtggcagttc tacaccgtcc gtctgctgtt gggcgtggcc gaggccggct
     6121 tttaccccag cgtgattctg tacctgacct attggtttcc caaccatcgc cgcgccaaaa
     6181 tgacggcttg ctttcaggcg ggcattccga ttgccggctt gattggaaat ccgctttccg
     6241 gttggctaat ggatacgttt catcatgttg ccggccactc gggatggcaa tggatgtttg
     6301 gtctcgaagc actgccgacg attcctttgg ccatcggcgc gctgctcatt ctcgacaatt
     6361 cggttagcgc cgcgcgttgg ctaagcccgc aggaaaagca attgatcgtc catgaactcg
     6421 gcaaggacga gaagacgcgc gagcacctgt cgctgctcaa agttttcacc gacggacgca
     6481 tctggaaaat tatcctgaca ggtttcccgg cgatgatggg cctctatgtc ctgggctttt
     6541 atctgcccac gctgatcaag gcatccggcg tgactacgag cacctcggtc ggctttctca
     6601 gcgcgattcc ctacctggtg gccgtgatcg ccatggtgac cttcgggcgc agctccgatc
     6661 gcatgcgcga gcgtcgctgg catctggcca tcgtgctgct gcttggcgcc gcgggcctca
     6721 ctgccagtgt ctggacaagc cacagcgttg tgctgacggt gatcaccatg tcgatcgccg
     6781 cggccggcat catcagctat tcgccgatca tgtggtcggt gccgacggcc tttctcggcg
     6841 gcgccgctgc ggcggccagc atcggcgtga tcaactccgc tatcaatatc ggcggcttcg
     6901 tgagccccta cctgatcggt tgggtcaagg acgaattcca cacgaccgcc ccggctatct
     6961 atatcgtcgc cggctgcctg acgttcgggg cattgatggc gctgagcttc gatcgcaagc
     7021 tggtcaatcg ctgagtgcgc gctccccaaa ccctatcccc ggagaagatt atgtcgacga
     7081 cagacaaacc gatgcgtttc gtcatgggtg gcatcatcca cgaaaccaat acctacgccg
     7141 cggatgtcgc gggccagacg cccctggcgt cgttcgagca atactatggc gacgcaattc
     7201 caggcatttt taccggctcc aatcatcagg tcggggggta tatcgagggc gcccgcaaac
     7261 acggcgctcg cctgatccat acgttcctcg ggcaagccac gccgtcgggc acgatagaag
     7321 gcaacgctta cgctcacatg aaacaggcga ttctcgcagg cattgagcag gcgttgcccg
     7381 ttgacggcgt gttgcttgcc ttgcatggcg cgggcgtggc cgatggcatc gaggacatcg
     7441 aaggggatct ggccgctgcc gtgcgcaccc tggtcggccc gcatgtaccg atcgcggcag
     7501 cctatgacct gcacggcaat atgaccgggc aaatgcagca cgccgtcgat atttcgctgc
     7561 cctgcattct ttatccccac acggattttt atgaccgtgg catcgagtgc gtcgacctca
     7621 tggtcaagat gatcaggggc gaggtgaagc cgatcacgca tgtctgtacc ttgccgatgc
     7681 tgccttacat cgtgaccacc aacgaaggct tcgtgccggc caaagtcaac gaactgtgtg
     7741 cccggctcga agcgctacct ggcgtgctcg actgctcgtg gtttcatggc tttccctatg
     7801 cggatatcgc atcgccgtgc ccggcggcga tttgcacgac caacggcgac ccggcgctgg
     7861 cga
//
LOCUS       DATASH010000046         3964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_202995,
            whole genome shotgun sequence.
ACCESSION   DATASH010000046 DATASH010000000
VERSION     DATASH010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202995"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(289..1101)
                     /locus_tag="VHV99_01495"
     CDS             complement(289..1101)
                     /locus_tag="VHV99_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX3634164.1"
                     /translation="MTTPSSADNPPPLDATPARALGEFIRAHRERLSPQALGLPPGPR
                     RRTPGLRREEVAQLCGVSPTWYTWIEQGRPVSASADALARIAVALQLSRAERAYLFEL
                     AAQRDPAEPDPAAADAPATLLETVQLVNAPAYVLDRQWNALAWNARAADLFVGWLDGT
                     HERNLLRYTFTQPAARELIFDWETRARRLAAEFRADSIRHLNDAPTRALIDSLTAASD
                     EFARFWASQDVGGREGGRREFNHPREGRIVYDQITFKPAHREDLKLVVLVRE"
     gene            1303..2055
                     /locus_tag="VHV99_01500"
                     /pseudo
     CDS             1303..2055
                     /locus_tag="VHV99_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017333860.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            2188..2520
                     /locus_tag="VHV99_01505"
     CDS             2188..2520
                     /locus_tag="VHV99_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634165.1"
                     /translation="MQRLIFLVVATVALPGAAWAQGNPVYHANPYTTLPRALSSDPVT
                     PDSTSGPISITTPGSNVNMSQQARRDLDARVQSERSERDKRVQAWREPPLKFNIPAPE
                     MPKPGAAN"
     gene            complement(2609..>3964)
                     /locus_tag="VHV99_01510"
     CDS             complement(2609..>3964)
                     /locus_tag="VHV99_01510"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEX3634166.1"
                     /translation="VTREKFGARAVRNYIISHTETVSDLVEVMLLQKETGLLHGRLGD
                     ANDPARAGLMVIPLFETIPDLRNAPHIMRDLIALPGVDTLIEHQGNEQEVMLGYSDSN
                     KDGGFLTSNWELYRAELALVSLFNQRGVTLRLFHGRGGTVGRGGGPTYQAILSQPPGT
                     VDGQIRLTEQGEVIASKFGNPEIGRRNLETVVAATLEASLLPHGNAPAQLPAFEETMQ
                     QLSDAAMASYRALVYETPGFKEYFFESTPISEIAELNIGSRPASRKLQDPKQRKIEDL
                     RAIPWGFSWGQCRLLLTGWYGFGSAVAAHLDSAPSDAERTRRLALLKKMHKTWPFFAN
                     LLSNMDMVLAKTDLAVASRYAQLVSDKKLRKHVFERIVAEWERTSKVLSEITGKSERL
                     AENPLLARSIKNRFPYLDPLNHLQVELLKRHRAGDTNARVRRGIHLSINGIAAGLRNT
                     G"
BASE COUNT          706 a         1247 c         1300 g          711 t
ORIGIN      
        1 tcgacgagcg ccagacgctt gtcgaagaaa accgacgacg tgtagcgttt gacgagctcc
       61 gacatcggct ccgagaagcg agccgaccag gcttcgcctt ttttgtgcag ttgggacgtc
      121 atggtgttgg gcagttaagc gagcggtgag gagactgacg gcgtttgcgt agggtgcggg
      181 cgccatcgaa acgggcgaaa gagggagatt ttaacactgg cggcccgcgg caaagatgcg
      241 gtctgccaag gggcggccct tggtgaaagg gcgccgccac gcaagcagct attcccgcac
      301 cagcaccacc agtttcagat cttcgcgatg cgcaggtttg aaagtgatct ggtcgtagac
      361 aatgcggccc tcgcgcggat ggttgaattc gcgccggccg ccttcacgcc cgccgacgtc
      421 ctgcgacgcc cagaagcgcg cgaattcgtc gcttgcggcg gtcagtgagt cgatcaaggc
      481 gcgtgttggc gcgtcgttca gatggcgaat cgagtcagcg cggaattccg cggccaggcg
      541 ccgggcgcgc gtttcccagt cgaaaatcaa ctcacgcgcg gctggctggg tgaaggtgta
      601 gcgcagcaga ttgcgctcgt gcgtgccgtc cagccagccg acgaacaggt cggcggcacg
      661 cgcgttccag gcgagcgcat tccactgccg gtcgagcacg taagccggtg cgttcacgag
      721 ttgcacggtt tcaagcagcg tggcgggcgc gtcggcggcg gctggatcgg gctcggccgg
      781 gtcgcgctgc gcggccagtt cgaacagata cgcccgctcg gcgcgcgaca gttgcaatgc
      841 aacggcaatc cgcgccagcg cgtccgcgga ggcggagacc ggccggcctt gttcgatcca
      901 cgtgtaccag gtcgggctca cgccgcacaa ctgcgcgact tcctcgcgcc gcaagccggg
      961 tgtccggcgg cgcggcccgg gtggcaggcc gagcgcttgc ggcgagagcc gttcacggtg
     1021 cgcgcggatg aactcgccga gtgcgcgggc gggcgtggcg tcgagcggcg gcgggttgtc
     1081 ggcggaggac ggcgtggtca ttcttgtcgg gcagcgcttg aagggggtgt cgaggtgatg
     1141 tcgggtggtg tcggggtggt gttatcgata ccagaataat tgcttaactt gtactggtac
     1201 acggcggcct ctattgtagc ggcaggaacg gcgcagtgcc cgtccagctc agtccgggcc
     1261 caagcccggc ccaaccccac agagacaaac cggagccccc ccatgaagca tcacgaccag
     1321 gtcgccgacg cctttggttc gaccgccgcc gcgtatttga cgagccagac acatgccacc
     1381 ggtgccgacc tgcagacgct ggccgagtcg atcgccgcga cgccgggcgc taaggtgctc
     1441 gacatgggct gcggcgcggg ccacgcgagt tttgcggtgg cgccgcacgc acaagaagtg
     1501 gtcgcatacg acatcgcgcc gcaaatgctg gcaacggtcg acggcgcggc gaaggatcgc
     1561 ggcctgacca acatccgcac gcaatagggc gcggccgaaa ggttgccgtt cgacgatcac
     1621 gcgttcgact ggatcatcag ccgcatgagc gcgcatcact ggcatgacgt gccgccggcg
     1681 ctcgccgaag tacggcgcgc attgaagccg ggtggcaaag tgctgttcat cgacatcgcc
     1741 ggtgcggatc atccgctgct cgacacgcat attcaggcgg tcgaagtatt gcgcgacggc
     1801 tcgcacatcc gcgactaccg cgccgacgaa tggattgcgt ttttcgaagc ggccggcttc
     1861 aaggcatcga ttcgcgaacg ttggcgcatc gacatcgaat tcacgtcgtg ggtggcgcgc
     1921 atgcgcacgc cggagccgcg tgtggtggcg attcgctcgc tgtggcgcaa cgcgcccgat
     1981 gaggtgcgcc actatttcga cgtgcaggaa gacggctcgt tcaagctcga cgcgctgatg
     2041 gtcgaggcgc agtaagcctg gaagagatgc gggcgccgat gcgtcggccg cccgtgcgac
     2101 cgaacgacac gtgtgcctca tcccggaaaa gttgccgcgt aagcgcgtgc tacgctgcgg
     2161 attgctaagc aattcggagg tatgcagatg caacggctga tctttcttgt ggtggctaca
     2221 gttgcattgc cgggcgctgc gtgggcgcag ggcaatccgg tctatcacgc caatccctac
     2281 acgacgttgc cacgcgcact gtcgtctgat ccggtcacgc ccgattcgac cagtgggccg
     2341 atcagcatca ccacgcccgg ttccaatgtg aacatgtcac agcaagcgcg ccgcgacctt
     2401 gatgcgcgcg ttcagtccga gcgctcggag cgcgacaagc gcgttcaggc atggcgcgag
     2461 cctcccctca aattcaacat tcctgcaccg gaaatgccga agccgggtgc ggcaaactga
     2521 tccgcacccg gcttcgtttt tcaagcggtt cccgcagagg ccgccggttg cggcgggtga
     2581 ttcaatacac ccgcctcatc tggcccgatc agccagtatt ccgcaatccc gccgcaatcc
     2641 cgttgatgct cagatgaatc ccgcgccgca cgcgcgcgtt ggtatcgccc gcacggtgac
     2701 gcttgagcaa ctccacctgc aagtggttca acgggtcgag atacgggaag cggttcttga
     2761 tcgaacgcgc gagcagcgga ttctccgcga gccgctcgct cttgccggtg atctccgaca
     2821 acaccttcga cgtccgctcc cattccgcga cgatccgctc gaacacgtgc ttgcgcagct
     2881 tcttgtccga cacgagctgt gcgtagcgcg acgcgaccgc caggtcggtc ttcgccagca
     2941 ccatgtccat gttggaaagc aggttcgcga agaacggcca ggtcttgtgc atcttcttga
     3001 gcagtgcaag acggcgggtg cgttcggcat cgctcggcgc gctgtccaga tgcgcggcca
     3061 ccgcgctgcc gaaaccgtac cagccggtca gcagcaaacg gcattgaccc catgagaagc
     3121 cccacggaat cgcgcgcaga tcttcgatct tgcgttgctt cgggtcctgc aatttgcgcg
     3181 aagccggacg gctgccgatg ttcagttcgg cgatctccga gatcggtgtc gactcgaaga
     3241 aatactcttt gaacccgggc gtttcgtaga ccagcgcgcg atacgacgcc atcgccgcgt
     3301 cggacaattg ctgcatcgtc tcttcgaagg cgggcagttg cgcgggcgca ttgccgtgcg
     3361 gcaacaacga ggcttccagt gtggcggcca ccaccgtttc cagattgcgc cggccgatct
     3421 ccggattgcc gaacttgctg gcaatcactt cgccttgctc ggtcaagcgg atctggccgt
     3481 cgacggtgcc cggcggctgc gacagaatgg cctgataagt cggaccaccg ccgcgcccaa
     3541 ccgtgcctcc gcgtccatgg aacaggcgca gggtcacgcc gcgttgattg aacagcgata
     3601 ccagcgcgag ttcggcacga tacaactccc agttcgaggt caggaagccg ccgtccttgt
     3661 tgctgtccga atagccgagc atcacttcct gctcgttgcc ctgatgttcg atcagcgtgt
     3721 cgacgcccgg cagcgcgatc agatcgcgca tgatgtgcgg cgcgttgcgc aggtcgggga
     3781 tcgtctcgaa gagcggaatc accatcaagc ccgctcgcgc cggatcgttt gcgtcgccga
     3841 gacggccgtg aagcagaccg gtttccttct gcagcaacat cacttcgacc agatcgctga
     3901 cggtctccgt gtgcgaaatg atgtagttgc gcactgcgcg cgcgccgaac ttctcgcgcg
     3961 tgac
//
LOCUS       DATASH010000047         9340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1984119,
            whole genome shotgun sequence.
ACCESSION   DATASH010000047 DATASH010000000
VERSION     DATASH010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 9340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9340
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1984119"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            282..989
                     /locus_tag="VHV99_01515"
                     /pseudo
     CDS             282..989
                     /locus_tag="VHV99_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493171.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
     gene            complement(1021..1359)
                     /locus_tag="VHV99_01520"
     CDS             complement(1021..1359)
                     /locus_tag="VHV99_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008353632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3331 domain-containing protein"
                     /protein_id="HEX3634167.1"
                     /translation="MHTKRFVGDRTGARAAAAPSFPQAIVRVIERLSSSKAVVYWSDV
                     SMCHYGYQDWREAVATTGGVCALSGRLIRRGDSVYRPSQRKPKPKNAAAMILSSAMPD
                     DEEIEVDVPG"
     gene            1847..2455
                     /locus_tag="VHV99_01525"
     CDS             1847..2455
                     /locus_tag="VHV99_01525"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX3634168.1"
                     /translation="MMRRPSSEAKREHLLDIAAERFLARGFAAVSIDELVATAGQSKT
                     NVYSWFGGKEGLFLAAVDRLLEALLIPLVDNDFAKLPLGDGVHALAETVLDIALRSDA
                     LALHRLIVSEAPSFPHIARAWMNAGPERTYALCADFILAHQKAGRLRKTDAGLTAMFF
                     VEMLIGDLERRMLIGERIKLARAERRTLVKAVADLFVHSYAA"
     gene            complement(2693..3457)
                     /locus_tag="VHV99_01530"
     CDS             complement(2693..3457)
                     /locus_tag="VHV99_01530"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3634169.1"
                     /translation="MTEASTKRIALVTGAGSGIGRATAMALLADGFCVVLAGRRAEPL
                     AELAETAKAVGQEALAVVTDVRDAASVDALFATIERTYGRLDVLFNNAGVNAPAVPMD
                     ELPVETWTNLINTNVTGVFLCARGAFGLMRKQSPQGGRIINNGSISAHAPRPFTSPYT
                     ASKHAVLGLTKSIALDGREFNIVCSQIDIGNALTELSERMTRGVRQANGTMAPEPMFD
                     VKHVAEAVRYIANLPLSTNVLNMTVMASNMPFVGRG"
     gene            complement(3642..5222)
                     /locus_tag="VHV99_01535"
     CDS             complement(3642..5222)
                     /locus_tag="VHV99_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX3634170.1"
                     /translation="MNTGKTTLSIRARLIVGFGVVLIALVALTIIAISRVEMVRTRLD
                     DIIDVNGVKERYAINFRGSVHDRSIAVRDVTLVSAAELPSVIDHIHALADQYDQVAAP
                     LAKIYAERTDITAQERAIYARVNAAQRQSTPLIQQLISLQQAGDVAGARKVLLDDARP
                     ALVEWLAGINALIDFQEQLNLQEGAEARKISTDFRLLMIVLTFVACGVGIAIVYALVR
                     NIMQALGAEPGEVVELAESIRAGNLARQIELRPGDTSSVMAAMARMQQRLAEIVTQVR
                     DAAQGMAAATEQIALGNADLSTRTERQALELEHASGALEKFDSSVAANTQSAGNADEL
                     AKHASATSAQGGAAVGQVVHTMHAISESSNRIEDIISVINSIAFQTNLLSLNAAVEAA
                     RAGTQGRGFAVVASEVRMLAQRCATAAKEIKELIATSSARIGEGENQVDAAGTTISNV
                     VEASRRVTHIMETIHQACLAQAHQIGEVRDVIGSLEQNTQQNVTLVQQSAAAAQTLKE
                     QAQGLLDAAQVFTLPGQN"
     gene            5695..5943
                     /locus_tag="VHV99_01540"
     CDS             5695..5943
                     /locus_tag="VHV99_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634171.1"
                     /translation="MLAWKGASIGGLIVGVILLDLGLQRAHVSNLARNLAVRSTVMNR
                     ANTLYMTIRFAGGAIGATLGNYAWSVWHWPGVRGVGLV"
     gene            complement(6078..6920)
                     /locus_tag="VHV99_01545"
     CDS             complement(6078..6920)
                     /locus_tag="VHV99_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011349790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3634172.1"
                     /translation="MSGLGIHALSVGYGRRQVLAALEAGPLPRGKITALLGPNGSGKS
                     TLLRALAGLTRASAGTLSLDGELLELSAANARSHSVVYLPQALPAGVRLQVLESVLVA
                     CRATRRQSFQLPRAAGHDDVTYAYAVLQRLGIDDLATRSLDELSGGQRQLVGIAQALV
                     RDPQVLLLDEPLSALDLNHQFHVMRVLHDVTRERDIVTIVVLHDINAAMRACERAMLL
                     HEGRIVRFGPPAEVVTPANLAKVFGVEARIEQCSRGHHQVLIDGLAGHTRIASDTASP
                     AASS"
     gene            complement(6917..7987)
                     /locus_tag="VHV99_01550"
     CDS             complement(6917..7987)
                     /locus_tag="VHV99_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003804421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX3634173.1"
                     /translation="MSTLQNHASLHAGSVFRYRRLTQRRVAALVAVTALIAASLLFDL
                     RTGPSGLPFDTLLRTVFDRASADPATSVIVWQIRLPYAVMALLVGASLGLSGAEMQTV
                     LNNPLASPYTLGVSAAAAFGASLAIVLDVAIPGVSQTWIVSANAFVFALLSALLLDAV
                     ARWRGMSTAGVVLMGIALVFAFHALVALMQFIASADALQGLVFWTLGSLARSDWPKIG
                     VLGLALALALPMSMRNAWRLTALRLGEDRAASFGIDVRRLRLGTLLRVSVLSALAVSF
                     VGTIGFIGLIAPHIARSVFGEDHRFYLPGSMLFGALMLSLASIASKLIIPGVLIPVGI
                     VTALVGIPLFLGIVVRSQGELG"
     gene            complement(7999..9231)
                     /locus_tag="VHV99_01555"
     CDS             complement(7999..9231)
                     /locus_tag="VHV99_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011349792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3634174.1"
                     /translation="MAIILIQNYIMSILRFSRARRPLTLAAGALTACVALTFGAFVTS
                     TGACAATQAPATQAPQTVTDLAGRTVAIPAGPHRILLGESRLLTAVALLEGQHPLARI
                     VGWQGDLPTMDPQTYDAYAKKFPEIKQIPLIGKATEDSISAEKALSLKPDLAIFSLGG
                     HGPGQYNALVKQLESTGTTVVFVDFRLHPMQNTLPSIKLLGTVLHREKEADAYIRFYQ
                     QHLAHVQSVVSQIPQNQRPKVFIDLLAGAWDAGCCHTAGNGNFGEFITTAGGRNIAAG
                     LVPGVLGDISMEQIIASRPDVYIATGSRTKPGLAALRVGALTSEQDAQASLAALVARP
                     GFGTIKAIHDGHVHGISHNYYDSPYNIVAIEAFAKWFYPQQFKDLDVHATQDELYRRF
                     LAVPPDGAYWVDAPRAAH"
BASE COUNT         1753 a         2830 c         2938 g         1819 t
ORIGIN      
        1 cgcctccggt ccgcggagat ccgcgtctta atgataccat acggtacggt attaatatta
       61 aatcgccgga ccgtttgaga tcgagcgagc acgcacctga atacacgcct gctcgcaaga
      121 acctggtccc aatatctctg aaacaggtct caagttcagc gcggtgttgc tcgatagacc
      181 gttgcagcgg atcaggcaga tgccacgtca aggaactgag ttacatcaaa acgagtcgtc
      241 tcttgcctga cgggcgacat gaaaggaatt gcaaaatggg aatgaagtcg accgtccgga
      301 gcctgactac cagcgctgca ctgatagcag tcatcggcct gggcggttgc gcgaccgggc
      361 ttggtcgcac acccggcgat ccgcttgagc cgatgaaccg tgcgattttt tcgttcaacg
      421 aagggctgga caaatacgtc gcgcgtccag ccgctgtggg atacacgaag gtgacgccga
      481 gtccgattcg tgtggcgata agcaattttt tttcgaatat cggcgatgtc ggcaacttcg
      541 ccaacgacct gctgcagttg aaggtcaccg atgccaccga agacctcgtg cgcttcgcgt
      601 tcaactcgac cttcggtatc ggtggcctga tcgactgggc gtcgccggcc ggattgccga
      661 aacataatca ggactttgga ctgacgcttg ggcattatgg cgtgccggcg ggtccgtatc
      721 tcgtgttgcc gctgttcggg ccgagttcgt tccgggacgc gaccaactgg gcattcgcgt
      781 acttcctgac gccgacaacc tatctgtccg cggaggcgag tgtgccgctg ttcggtctgg
      841 attttgtcag tacgcgggcg accctcctgg gcgcgacgga cattctgtcg caggtggcgc
      901 tggataaata cacgtttgta cgcgatgcct atgcccagcg ccgtcagtcc atgctgaaga
      961 gcgacgctgc gccgccgcag tatgtggatg atagccgcgg agaggccggg ccttgaggcg
     1021 ttagccgggg acgtcgactt ctatctcttc atcgtccggc attgccgacg agagaatcat
     1081 tgccgctgcg tttttcggtt ttggtttgcg ctgcgacggg cgatacacgg agtccccacg
     1141 acgaatcagc cggccggaca gcgcacagac accaccggtt gtcgcgaccg cctcgcgcca
     1201 atcctggtat ccatagtggc acatgctgac gtcggaccag tacacgaccg ctttactact
     1261 acttaaccgt tcgatcacgc gcacaatcgc ttgcggaaat gacggagcag cggcagcccg
     1321 agcgccggtt cggtcgccga caaaccgttt cgtgtgcatg cgttcagtcg ttactatttt
     1381 cgcgagacgg ggcgccgcag tctccacagc ggggagtgcc accccgtacc ccgcatctcc
     1441 gatcttcgat aatgtgctca tggcgttcct cgccggatct cgatattcag aatggttctc
     1501 cctgatgctc gtgctcaaag gcgatgtcct gagagtagtc ccgtgcgtaa gtggcctgta
     1561 tgcgagagca tgcccgcgct cctgcgcgta taccgtaccg tatggtatca ttgtatcaat
     1621 caatgacggg attgtgatcg actcaggtga cccggtgcgc ttcctgaaac ttcgactgac
     1681 tcggcttttt gctgggtaga aatcctacgt caatcatcgg ctagcgcgta cggtgcgagg
     1741 cgcggcaagt tggacggccc ttcttggccg aatcctgact tccgcgctct caggcgcgat
     1801 tttgttgccg cgtccagcct gatcggccgc ggcgtgaccg gggactatga tgaggcgacc
     1861 cagttccgaa gcaaagcgcg agcatcttct ggacattgca gcagaacgct ttctcgctcg
     1921 agggtttgcc gctgttagca tcgacgaact cgttgctacc gcgggccaat cgaagaccaa
     1981 tgtgtattcg tggttcggtg gcaaggaagg cctgtttctc gctgccgtgg acaggctgct
     2041 tgaggcactg cttattccgc tcgttgacaa tgatttcgcg aagctgcctt tgggcgatgg
     2101 ggtgcacgcg ctggccgaaa ctgttctgga tatcgctctt cgatcagacg cgctggcatt
     2161 gcatcggcta atcgtctctg aggctccctc gtttcctcac attgcgcggg catggatgaa
     2221 cgctggtcca gaacggacat acgcgctttg cgccgacttc atactggccc accaaaaagc
     2281 gggccggctc cgcaagacgg atgccgggct cacagcgatg ttcttcgtcg aaatgttaat
     2341 cggcgatctt gagcgacgga tgttgattgg tgaacggatc aagctcgcgc gagcggaacg
     2401 aaggacgctc gttaaagcag tcgctgatct tttcgtacac agttatgcag cgtgagccgg
     2461 caagcacctg gtgatgccgt gacaacggct gcggtaatag cgggatgccc agctgaagct
     2521 gggcacgcgg gcctccgcgc tggcgattct ccgaaggcac tgccgttcag gtccgccagc
     2581 gaggggtgaa tctggcggcc gatgaactcc ggatggagct tgatagaaga ctgtcggctt
     2641 tcacgagctt ctcgaagacg atgtaagaga aatgctcgcc tgcaggcagg gcctagccgc
     2701 gccccacaaa tggcatattg ctagccataa ccgtcatgtt cagaacgttg gtgctcaggg
     2761 gtaggttggc gatgtaacgt accgcttctg caacgtgctt cacatcaaac atcggttccg
     2821 gcgccatcgt gccatttgcc tggcgtacgc cgcgcgtcat gcgctcggac agttcagtta
     2881 gcgcattgcc aatgtcgatc tgactgcaga cgatgttgaa ctcgcggcca tcgagcgcga
     2941 tactcttcgt gagaccaagc actgcgtgct tgctagccgt gtagggcgaa gtgaaggggc
     3001 gcggcgcatg tgccgagatc gacccgttgt tgataatccg gcccccttgc ggtgattgct
     3061 ttctcatcaa tccgaaagca ccgcgagcgc agaggaaaac accggtcacg ttggtattga
     3121 tgagatttgt ccaggtctca acgggcagct catccatcgg cacagccggc gcattgacgc
     3181 cagcattgtt gaacagaacg tcgagcctgc cgtaggtgcg ctcaatggtg gcgaaaaggg
     3241 catcgacact tgctgcgtcg cgaacgtcgg tcaccacggc gagggcttcc tggcccacgg
     3301 cctttgctgt ctcggcgagt tcagcgagcg gctcggcacg gcggccagca agcactacgc
     3361 agaagccgtc cgcgagaagc gccatcgcag tggcccggcc aatgccactg cctgcgccgg
     3421 taaccagtgc gattctcttg gtgcttgctt cagtcatgat cttatctccg tgatttgatg
     3481 aacgctgttg aggcgattgt tagctcaagg atacgcagta aacgttgacg caacctggac
     3541 gcaacggtgt gcgtcctgga cagatcaacg caagttgggc agtgtgcaac cgggtgcttg
     3601 ccttggcacc agagcctgaa tatgacgacc ggctctttta attagttctg acctggcagc
     3661 gtgaatactt gcgccgcgtc cagcaaaccc tgcgcctgtt ccttcagcgt ctgcgccgcg
     3721 gcagcggact gctgcacgag cgtcacattt tgttgcgtgt tctgctccag actgccgatc
     3781 acgtcgcgca cttcgccgat ctgatgcgcc tgcgcaaggc aagcctgatg gatggtttcc
     3841 atgatgtgcg tgacgcgacg cgatgcttcc acgacgttgg agatggtcgt gccggccgca
     3901 tcgacctggt tctctccttc gccaatccgc gcgctgctcg tggcgatgag ttccttgatt
     3961 tccttcgcgg cagtggcaca gcgctgtgcg agcatgcgca cttcgcttgc gacgaccgca
     4021 aagccgcggc cctgggtgcc ggctcgtgca gcttccaccg cggcattcaa tgacagcaag
     4081 ttagtttgga atgcgatcga attgatgacg gagatgatgt cctcgatgcg attcgacgat
     4141 tcgctgatcg cgtgcatcgt gtggacgacc tgcccgaccg cggcgccgcc ctgggccgag
     4201 gtggccgaag cgtgcttggc gagttcgtcg gcattgcctg cgctttgcgt attggccgcg
     4261 acgctggagt cgaacttttc gagcgcgccg ctcgcgtgtt cgagttccag ggcctgccgc
     4321 tcggtgcggg tgctcaagtc ggcgttgccc agcgcgattt gctccgtggc agccgccatg
     4381 ccctgcgcgg cgtcgcgcac ttgcgtgacg atttcggcca gcctttgctg catgcgcgcc
     4441 atcgcggcca tcacgctcga cgtatcgccc ggccgcaatt cgatttggcg cgcgaggttg
     4501 cccgcacgga tcgactccgc cagttccacc acttcgcccg gctcggcccc gagggcctgc
     4561 atgatgttac gcacgagcgc ataaacgata gcgatgccca ctccgcacgc aacgaacgtc
     4621 agtacgatca tcaacaagcg aaaatcggtg ctgatcttac gcgcctcggc tccttcctgc
     4681 agattgagct gttcctgaaa gtcgatcagc gcgttgatcc ccgccagcca ttcgacgagc
     4741 gcgggacgcg catcatccag caaaactttg cgcgcgcccg cgacatcgcc cgcttgttgc
     4801 aagctgatca gctgctggat cagcggtgtg ctttgccgct gcgcggcatt gacccgtgca
     4861 tagattgcgc gttcctgcgc ggtgatgtcc gtgcgctcgg catagatctt cgcaagcggt
     4921 gcagccacct ggtcgtactg gtccgcaagc gcatgaatgt gatcgatgac cgaaggcagc
     4981 tccgccgcgg aaaccagcgt cacgtcgcgc actgcgatgg agcggtcgtg gacgctgcca
     5041 cgaaaattga tcgcgtaacg ttccttcacg ccgttcacgt cgatgatgtc atcgagccgc
     5101 gtgcggacca tctcgacccg gctgatcgcg atgatcgtca gcgcgacgag cgcgatgagg
     5161 accacgccga aacctacgat caaccgcgcc cgaatgctca atgtcgtctt acccgtgttc
     5221 acgaaagttc cccgtctgct ggcgcagcct tgacctgcat ggccgttaag ccggaatgcg
     5281 gcgcgccgtt tgtgtttgaa gaaggcatta taaccaacaa aagctatgaa aaaggctaga
     5341 ttgatagttt ttgtgatgtg cctcgaggcc gagttgcgaa atctcggtcg gcgatctgcg
     5401 acgaacgatc tttgatcatt cggaagggag aacagccagc aagtcgccgg gcggcagcct
     5461 gtaaatcgca actaaccgtc tgacgccact ggcgcccacc accaacgacg gccgccggcc
     5521 cgcggccttc agacggacgt ctctccgcac gtagaacctc acgactcccg acggtctgcg
     5581 tgcgggtggg tcgccggcgc gagcgcctcc tacgctgcgg gaaagcagac cgatgcgcgc
     5641 gatccgcgtc gtgtcatcct cgccgccggc atcgtcttca tcgcctcgta tgcgttgctt
     5701 gcctggaagg gcgcgagcat cggcgggctc atcgtcggcg tgatcctgct cgacctcggc
     5761 ttgcaacgcg cgcatgtctc gaacttggcg cgcaacctgg cggtgcggag cacggtgatg
     5821 aaccgcgcca acacgcttta tatgacgatc cgttttgcag gcggagcgat cggcgcgacg
     5881 cttggcaact atgcgtggag cgtgtggcat tggccgggtg tgcgcggcgt tgggcttgtc
     5941 tgatagtttg aggcggtcgc gacccgacgc gacgacccga cacgacgaca cgacgacacg
     6001 acgacgcgac gcccgcggag cgggcgccgg tcgacggctg gaatagcggt ctgaagctgg
     6061 gctggctggg ccgcgtgcta actactggcg gcggggctgg cggtatcgct ggcgatgcgc
     6121 gtgtgaccgg cgagaccgtc gatcagcact tgatggtgtc cgcgcgagca ctgctcgatt
     6181 cgcgcttcca ctccaaaaac tttggcgaga ttcgccggcg tgacgacttc ggcgggtggt
     6241 ccgaagcgga cgatgcgtcc ttcgtgcagc aacatcgcgc gctcgcatgc gcgcatcgcg
     6301 gcgttgatgt cgtgcagcac cacaatggtc acgatgtcgc gttcgcgcgt gacgtcgtgc
     6361 agcacgcgca tgacgtgaaa ctgatggttg agatcgagcg ccgaaagtgg ttcgtcgagc
     6421 agcagcacct gcgggtcgcg caccagtgcc tgggcgatac cgacgagctg gcgctggcct
     6481 cccgacaact cgtcgagtga acgcgtggcg aggtcgtcga tgccgagcct ttgcagcacg
     6541 gcatacgcat acgtgacgtc gtcgtgccct gcggcccgtg ggagctggaa cgactggcgc
     6601 cgcgtggcgc ggcatgcgac cagcacggat tcgagcacct gcagccgcac gcccgccggg
     6661 agtgcctgag gcagatacac gacgctatgc gagcgggcat tggccgccga caactcaagc
     6721 agttcgccgt cgagtgaaag cgtcccggcg cttgcgcgcg tgagcccggc aagtgcgcgc
     6781 agcaacgtgg acttgccgct gccgttcggc cccagcagtg ccgtaatctt gccgcgcggc
     6841 aacgggccgg cctcgagcgc ggcgagcacc tgtctgcggc catacccgac actcaatgcg
     6901 tgaataccga gcccgctcat cccagttctc cctgtgagcg cacgacaatg ccgagaaaga
     6961 gcggaatgcc gaccagcgcc gtgacgatac cgaccggtat cagcacaccg ggaatgatca
     7021 gcttcgaggc aatggatgcg agcgacagca tcagcgcgcc aaacaacatg ctgccgggca
     7081 gatagaagcg gtgatcttcg ccgaacaccg agcgcgcgat atgcggcgcg atcagcccga
     7141 taaacccgat cgtcccgaca aacgaaaccg ccagcgccga cagcaccgac acgcgcagca
     7201 gcgtacccag ccgcagccgc cgtacgtcga tgccgaaact ggcggcgcga tcttcgccta
     7261 gccgcagcgc ggtgagccgc caggcgttgc gcatcgacat gggcagcgcc agcgcgaggg
     7321 cgagcccgag gacgccgatc ttcggccagt cggagcgggc gagcgagccg agggtccaga
     7381 agacgaggcc ttgcaaagca tcggcggaag cgatgaactg catcagcgca accagcgcgt
     7441 ggaaggcgaa taccagcgcg atgcccatga gcacgactcc cgccgtgctc atgccgcgcc
     7501 agcgcgcgac agcgtcgagc agcagcgccg acagcagtgc gaatacaaag gcgttggccg
     7561 acacgatcca cgtctgcgaa acgccgggaa tcgcgacatc cagcacgatt gccagcgacg
     7621 ccccgaacgc cgccgccgcc gacaccccga gcgtataagg gctggcgagc ggattgttga
     7681 gcacggtctg catctcggcc ccggacaggc caagcgatgc gcccaccagc aacgccatga
     7741 ctgcgtacgg caggcggatc tgccagacga tgacacttgt cgccggatcg gccgaggcgc
     7801 gatcgaacac cgtgcgcagc agcgtgtcga acggcagccc cgacggcccc gtgcgcaggt
     7861 cgaacagtag cgaggccgcg atcagcgccg tcacggcgac aagcgcggcg acgcgccgct
     7921 gcgtgaggcg ccggtagcgg aacacgctgc cggcgtgcag acttgcgtga ttctgaagtg
     7981 tggacatcgt gtggaagctc agtgcgctgc gcgtggtgcg tcgacccagt aggcgccatc
     8041 cggtggcacg gcgaggaagc ggcgatacag ttcgtcctgt gtcgcgtgca cgtcgaggtc
     8101 cttgaactgc tgcggataga accacttcgc gaaggcttcg atggccacga tgttgtacgg
     8161 cgaatcgtaa tagttgtgcg agatgccgtg cacgtggccg tcgtggatcg ccttgatcgt
     8221 gccaaagcca ggccgcgcga caagggcagc cagactcgct tgcgcatcct gctcactcgt
     8281 cagcgcaccg acacgcagcg cagcgagtcc cggttttgta cggctgccgg tcgcgatgta
     8341 cacgtccggg cgcgacgcaa tgatctgctc catgctgatg tcgccgagca caccgggaac
     8401 gaggcccgct gcgatattgc ggccacccgc tgtcgtgatg aactcgccga agttgccgtt
     8461 gcccgccgtg tgacagcagc ctgcgtccca tgcgcccgcc agcagatcga tgaagacctt
     8521 cggccgctgg ttctgcggaa tctggctcac gacgctctgt acgtgcgcca ggtgctgctg
     8581 gtagaagcgg atgtaggcgt cggcttcctt ctcacgatgc agcacggtgc ccaacagctt
     8641 gatgctcggc aacgtgtttt gcatcggatg cagacggaag tcgacgaaaa cgaccgtggt
     8701 accggtcgat tccagttgct tcacgagagc gttgtattgg cccggcccat ggccgcccag
     8761 gctgaaaatc gccagatcgg gcttcaggct cagtgccttt tcggcgctga tgctatcttc
     8821 agtggccttg ccgattagcg ggatctgttt gatctccgga aacttcttcg cgtaggcgtc
     8881 gtaggtttgc gggtccatgg tcggcagatc gccttgccag ccaacgatgc gtgccagcgg
     8941 atgctgacct tcgagcaggg ccaccgccgt gagcagccgg ctttcaccca gcaggatacg
     9001 gtgcggcccg gcgggaatcg cgacggtgcg gcctgcgagg tcggtgaccg tctgcggtgc
     9061 ctgcgtggcc ggcgcctgcg tggcagcgca cgcgccggtg ctcgtgacga acgcgccaaa
     9121 ggtgagcgcg acgcaggccg tcaatgcgcc agccgcaagc gtcaagggcc ggcgagcccg
     9181 agaaaagcgg agaatcgaca tgatatagtt ctgaatgaga atgattgcca tttgcgggtc
     9241 ctactttagc ggcgcgatgt aatggcaatg tgttgaaaca ctgctgtttt gtaatggaat
     9301 gtgttgagcg tggtgacgct ctgcataggc cacgtaaaca
//
LOCUS       DATASH010000048         9628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1390721,
            whole genome shotgun sequence.
ACCESSION   DATASH010000048 DATASH010000000
VERSION     DATASH010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 9628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9628
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1390721"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VHV99_01560"
     CDS             <1..318
                     /locus_tag="VHV99_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009913510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX3634175.1"
                     /translation="LILFNGNDMDEWHRAEAFGCDILEACVELGGTVTGEHGVGIEKI
                     NSMCVQFSPEERDAFHAVKRAFDAPGLLNPDKGIPTRARCAEYGKMHVRGGLLPHPDL
                     PRF"
     gene            468..1556
                     /gene="glcE"
                     /locus_tag="VHV99_01565"
     CDS             468..1556
                     /gene="glcE"
                     /locus_tag="VHV99_01565"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HEX3634176.1"
                     /translation="MEEDDIVAVWSERVRSASAEGRTLRIRGGGTKDWYGQTLEGDIL
                     DTRAYRGIIAYDPAELVITARAGTPLLEIEAALAEHHQMLAFEPPHFGPQATFGGCIA
                     AGIAGPRRPAAGAARDFVLGAVIMNGQGQTLHFGGQVVKNVAGYDVSRLMAGSLGTLG
                     LILELSVKVLPLPQAEATLKFEMNGTDAVRKLNEWGGRALPITASAWRHGTLAVRMAG
                     AEAAVKAARTALGGEVVDAVEAERFWAGLREQTDSFFAGIPPKAALWRLALPSITEPL
                     QLPGAQLMEWGGGQRWWITDTDAQTVRISAKQAGGHATIFRTGLGYDRSAGIFTPLPA
                     PLMKIHRGLKTAFDPARIFNRGRLYPDF"
     gene            1565..2791
                     /gene="glcF"
                     /locus_tag="VHV99_01570"
     CDS             1565..2791
                     /gene="glcF"
                     /locus_tag="VHV99_01570"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HEX3634177.1"
                     /translation="MQTNLADFIRNTPDGDEADAILRNCVHCGFCTATCPTYQILGDE
                     LDGPRGRIYLIKQMVEGAAVTRSTQLHLDRCLTCRSCETTCPSGVQYGRLVEIGRKIT
                     EEQVTRPLGQRMLRRLLASFVPNSALFTPIMRTGQHIRPLLPKKLRDKVPARQRPLEW
                     PTAKHPRKMLMLTGCVQPSMMPNVNVATARVLNALGVETLVAPEAGCCGAIRLHLGYN
                     DEALDDLRANIDAWWPYIEQGVEAIVMNASGCGATVKEYAHLLRNDPAYAEKARRVTE
                     LTRDIAEILPEFEEGLVAVTRRRAIHTVAFHPPCTLQHGQQVRGKVEHLLAALGVEVR
                     LPADNHLCCGSAGTYSLTQPKLSYALRDQKLERLQAQEPQVIVSANVGCIAHLQSGTS
                     TPVAHWIELVEHMLSA"
     gene            2957..3655
                     /locus_tag="VHV99_01575"
     CDS             2957..3655
                     /locus_tag="VHV99_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015001649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEX3634178.1"
                     /translation="MPDLIHNLEAVQQRIALAAQVAGRDPRSITLLAVSKTFPAEDVR
                     AAHTAGQRAFGENYVQEAITKMEALADLRTALEWHFIGPLQSNKTRPVAESFDWVHSV
                     DRLKIAQRLSEQRPDNLPPLNVCLQVNISGEASKSGLSIPEAVEVAQEIATLPKLTLR
                     GLMAIPEPAGSIEEQRVPHRQLRELFERLRNDGLELDTLSMGMSSDLEAAVLEGATIV
                     RVGTAIFGARDYSN"
     gene            3707..4522
                     /gene="proC"
                     /locus_tag="VHV99_01580"
     CDS             3707..4522
                     /gene="proC"
                     /locus_tag="VHV99_01580"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEX3634179.1"
                     /translation="MKIAFIGGGNMGAALIGGLIKRGVAPADLYAIDPNEDARKRNEQ
                     QFGIKTGAAADAALASYDAVVLAVKPQILKSVAEALAPYLNASQLAISIVAGIRMEDM
                     SRWLNGHARIVRVMPNTPALIGMGVTGLVATGSVDETGRALASQVLGAVGETVWFDDE
                     AKIDAVTAISGSGPAYVFYFIEALQEAARQLGMDEAQGRALAVATFTGAAQLAANSDE
                     PPSLLRERVTSKGGTTAAALASFEANGIKDAIVRGALAADARAREMGDEFGKL"
     gene            complement(4563..5426)
                     /gene="ubiA"
                     /locus_tag="VHV99_01585"
     CDS             complement(4563..5426)
                     /gene="ubiA"
                     /locus_tag="VHV99_01585"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEX3634180.1"
                     /translation="MFARLPLYLRLVRMDKPIGSLLLLWPTLNALWIASDGHPSWPLL
                     VIFTVGTVLMRSAGCAINDYADRDFDRYVKRTENRPITSGKIKAWEAVALAAGLSLLA
                     FLLILPLNTLTKELSVAALFVAGSYPFTKRFFAIPQAYLGIAFGFGIPMAFAAIQDHV
                     PMLAWVMLLANVFWSVAYDTEYAMVDRDDDIKIGIRTSALTFGRFDVAAIMLCYAVTL
                     GIYVGIGVMLGFGVLYWLGWAAAVGCAIYHYTLIRNRERMACFAAFRHNNWLGGALFV
                     GIAAHYALASF"
     gene            5442..5672
                     /locus_tag="VHV99_01590"
     CDS             5442..5672
                     /locus_tag="VHV99_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634181.1"
                     /translation="MQTVPLFYGMWAAGSLLGRRGSKSLTCKSKTMPTLMLSPKPKPP
                     LSVQTGAAKNKTSREGEALFVFQFNANADALR"
     gene            complement(5697..6185)
                     /locus_tag="VHV99_01595"
     CDS             complement(5697..6185)
                     /locus_tag="VHV99_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HEX3634182.1"
                     /translation="MAKKEAVQHVNIGINDKDRKKIAEGLSRLLADTYTLYLKTHNFH
                     WNVTGPMFNTLHLMFETQYNELALAVDSIAERIRALGVHAPGTYKDFAKLSSIPEADG
                     VPAAEEMIRQLVEGQEAVVRTAREIFPSTEAANDEPTADLLTQRMQTHEKTAWMLRSM
                     LA"
     gene            complement(6336..6497)
                     /locus_tag="VHV99_01600"
     CDS             complement(6336..6497)
                     /locus_tag="VHV99_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634183.1"
                     /translation="MIQMMMSTIGGSPAARNARQQQALMLRKAVGFAIVSSGLAVIVA
                     QALQHALGA"
     gene            complement(6584..8038)
                     /locus_tag="VHV99_01605"
     CDS             complement(6584..8038)
                     /locus_tag="VHV99_01605"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HEX3634184.1"
                     /translation="MSERNLTNAAGAPVADNQNSMTAGARGPVVLQDVWLLEKLAHFD
                     REVIPERRVHAKGSGAFGTLKVTHDISRYTKAKVFAQVGKDTPLFMRFSTVAGERGAA
                     DAERDVRGFSIKFYTEEGNWDVVGNNTPVFFIRDPLKFPDFIHTQKRDPYTNMRSNIA
                     AWDFWSRHPESLHQVTILMSDRGIPQSYRQMHGFGSHTYSFINANNERFWVKFHFKSM
                     QGVENFTDAEAAQVVAQNRESAQHDLFTNIEAGNFPAWRFAIQVMPEADAAAYRFNPF
                     DVTKVWSQKDYPLIDVGTIELNRNAANYFADVEQAAFTPANVVPGIGFSPDRLLQGRL
                     FSYGDTQRYRLGINHHQIPVNAPRVPSPHSFHRDGAMRTDGNLGGNVNYEPNRFSDFA
                     QDSNASEPPLAAGTVDRYDHRQDGDYYTQPGMLFALFDAAQRQRLFGNIARHINGVPQ
                     EIVARQIEHFRRADPAYAEGVIAALADLAEGGKK"
     gene            complement(8109..8249)
                     /locus_tag="VHV99_01610"
     CDS             complement(8109..8249)
                     /locus_tag="VHV99_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634185.1"
                     /translation="MTQSNSQPVLTVAVQSARAITDSFAASARSLRTAALSLLVDRAL
                     SA"
     gene            complement(8454..9413)
                     /locus_tag="VHV99_01615"
     CDS             complement(8454..9413)
                     /locus_tag="VHV99_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017333233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-inducible genes activator"
                     /protein_id="HEX3634186.1"
                     /translation="MTLTELKYIVAVARERHFGRAAEACFVSQPTLSVAIKKLEDELN
                     VQIFERGTSEVSVTPIGEQIVTQAQRVLEQTLAIKEIAKQGKDPLVGPLRLGVIYTIG
                     PYLLPTLVKQMIKRVPQMPLMLQENYTIKLIELLKQGEIDVAVMALPFPETGLMLRPL
                     YDEPFVVALPSGHAWENRAKIDAEDLKQETMLLLGSGHCFRDHVLGVCPELMRFSQNA
                     DGIQKTFEGSSLETIRHMVASGVGITVLPRMSVNEVKPHAGGIDAGLLSYVPFGEPVP
                     DRRVVLAWRKSFTRMPAIEAISDAISACDLTGVKKLDLPVAVN"
BASE COUNT         1853 a         3024 c         2995 g         1756 t
ORIGIN      
        1 ctaattctgt tcaacggcaa cgatatggac gagtggcatc gcgctgaggc gttcggttgc
       61 gacattctcg aagcgtgcgt cgaactgggc ggcacggtga ccggcgagca tggcgtgggc
      121 atcgagaaaa tcaattcgat gtgtgtgcag ttctcacccg aagagcgcga cgcgttccat
      181 gcggtcaaac gcgcctttga cgcgccgggc ctgctcaatc ccgacaaggg catccccacc
      241 cgcgcccgct gcgccgagta cggcaagatg catgtgcgcg gcggcctgct gccgcatccg
      301 gacctgccgc ggttttaagg cgcctgcccc cctccagcgc agcgcgcata gcgcgcgccg
      361 gcgcggcttc ccggcttcca ccctgattac ccgatgcggg tccgctccgg gttgccagcg
      421 cgcccccgcc cggtacaatc agtcgaaaca caacgaagca gggcaccatg gaagaggacg
      481 acatcgtcgc cgtttggtcc gaacgcgtac gctcggccag cgccgaagga cgcacattgc
      541 gcatccgtgg cggcggtacc aaagactggt acggtcagac gctggaaggt gacatcctcg
      601 acacgcgcgc ttatcgcggc atcatcgcct acgatccagc cgaactggtc atcacggcgc
      661 gcgcgggcac gcccctgctg gagatcgagg ccgcgctcgc cgaacaccac cagatgctcg
      721 ccttcgagcc accccacttc gggccccagg ctaccttcgg cggctgcatc gcggcgggca
      781 tcgccggtcc gcgccgcccg gccgcaggcg cggcgcgcga cttcgtgcta ggcgccgtca
      841 tcatgaacgg ccagggccag acgctgcatt tcggcggcca ggtggtgaag aacgtcgccg
      901 gttacgacgt ctcccgtttg atggcaggtt cgctcggcac ccttgggctg atcctcgaac
      961 tgtcggtcaa agtgctgccg ctgccgcagg ccgaagccac gctcaaattc gagatgaatg
     1021 gcaccgacgc ggtccgcaag ctcaacgaat ggggcggccg ggcactgccg atcaccgcga
     1081 gcgcgtggcg tcacggcacg ctggccgtgc ggatggccgg cgcggaagcc gcggtcaaag
     1141 cggcacgcac ggcgctcggc ggcgaagtcg tcgatgccgt ggaggcggag cgcttctggg
     1201 ccggcctgcg tgagcagacc gactcgttct ttgcaggcat cccgccaaag gccgcactct
     1261 ggcgtctcgc gctgccgtcg atcaccgagc cgctgcaatt gcccggcgcg caactgatgg
     1321 aatggggcgg cggccaacgc tggtggatca ccgacaccga cgcgcaaacc gtgcgtatca
     1381 gcgccaaaca ggccggtggt cacgccacca tctttcgtac cggcctcggc tacgaccgca
     1441 gcgccggcat cttcacgccg ctccccgcac cgctgatgaa aatccatcgc ggcctgaaaa
     1501 ccgccttcga cccggcccgc atcttcaacc gcggccgcct ctaccccgac ttctgagcga
     1561 cgcgatgcaa accaacctcg cggacttcat tcgcaacacg cccgatggcg acgaagccga
     1621 cgccattctg cgcaattgcg tgcattgcgg tttttgcacg gctacctgcc cgacttatca
     1681 gatactcggc gacgaactcg atggtccgcg cggtcgcatt tatctgatca agcaaatggt
     1741 ggaaggcgcc gccgtcacgc gcagcacgca gctgcatctg gaccgctgcc tcacctgccg
     1801 cagctgcgaa acgacctgcc cgtccggtgt ccaatatggc aggctggtcg agatcggccg
     1861 caagatcacc gaggaacagg tcacgcgtcc gctcggtcag cggatgctgc gccggctgct
     1921 cgcgagcttc gtgccgaaca gcgcgttgtt cacgccgatc atgcgcaccg gtcagcatat
     1981 tcgcccgctt ctgccgaaga aactgcgcga caaggtgccc gcgcggcagc gtccgctcga
     2041 atggccgacc gcgaaacatc cgcgcaagat gctgatgctc acgggttgcg tgcaaccgtc
     2101 gatgatgccg aacgtcaacg tcgcgacggc gcgggtgctc aatgcgctcg gcgtggaaac
     2161 attggtcgcg cccgaagccg gctgttgcgg tgcgatccgt ctgcacctgg gctacaacga
     2221 cgaagcgctc gacgacctgc gcgccaacat cgacgcgtgg tggccctata tcgaacaggg
     2281 tgtggaagcg atcgtgatga acgcgtccgg ctgtggcgcg acggtcaagg aatacgcgca
     2341 tctgctgcgc aacgatcccg cctatgcgga gaaggcacgc cgcgtgaccg agttgacgcg
     2401 cgacatcgcg gagatcctgc cggagttcga agaggggctc gtcgccgtga cgcgccgccg
     2461 cgcgatccat acggtggcgt tccatccacc gtgcacgttg cagcacggtc agcaggtgcg
     2521 cggcaaggtc gaacatttgc tggcagcgct cggcgtggaa gtgcgtctgc ctgccgacaa
     2581 tcatctctgc tgcggctcgg ccggcactta ttcgctaacg cagcccaagc tctcatacgc
     2641 actacgcgat cagaaactcg agcggctgca ggcgcaggag ccgcaggtga tcgtgtcggc
     2701 gaacgtcggc tgcattgccc atctgcaaag cggcacgtcc acaccggtcg cgcactggat
     2761 cgaacttgtc gaacacatgc tgtccgcttg aagcgatcga atcgatgcaa gcgcggcatg
     2821 cggcttgcgg catgcggtgc cgcaccgcga gcggcttcgc catcctgcgc cggcatcgag
     2881 cggcatcaag cggcatcaag cggcggcgtc cgtataattc gagcattgcc tttcgctgga
     2941 cttccggttc cgttccatgc ccgatctgat tcataacctc gaagcggtgc agcagcgcat
     3001 cgccctcgcc gcgcaggtgg ccggacgcga cccgcgttcg atcaccctgc tcgcggtctc
     3061 gaagaccttt cccgccgaag acgtgcgcgc cgctcacaca gccggtcagc gcgcgttcgg
     3121 tgaaaactac gtgcaggaag ccatcaccaa aatggaagcg ctggctgatc tgcggacagc
     3181 gctcgaatgg catttcatcg gaccattgca atcgaacaag acgcggccgg tcgccgagag
     3241 tttcgactgg gtgcattcgg tcgaccgcct gaagatcgcg cagcggctgt ccgagcagcg
     3301 cccggacaac ctgccgccgt tgaatgtctg cctgcaggtc aacatcagcg gcgaagcgtc
     3361 gaaaagcggc ctgagtattc ccgaggccgt ggaggtcgca caggaaatcg ccacgctgcc
     3421 gaagctgact ctgcgcggcc tgatggcgat tccagaacca gccggcagca tcgaagaaca
     3481 gcgcgtgccg catcggcaac tgcgtgaact gttcgagcgt ctgcgcaatg acggcctcga
     3541 actcgatacg ctctccatgg gcatgtcgag cgacctcgaa gccgccgtgc tggaaggcgc
     3601 gacgatcgtt cgcgtcggca ccgcgatctt cggcgcgcga gattactcta actgaccgtt
     3661 cagacctttc cttcttgacg cctcacggcc cctctttcgg aacatcatga aaattgcttt
     3721 tatcggcggc ggcaacatgg gcgccgcgtt gatcggcggc ctcatcaaac gcggcgtcgc
     3781 gcccgctgac ctgtatgcga tcgaccccaa cgaggacgcg cgcaagcgca atgagcagca
     3841 attcggcatc aagaccggcg ccgccgcgga cgccgcgctc gcgtcgtacg acgcggtcgt
     3901 gctggcggtg aagccgcaga tcctgaagag cgtcgccgaa gcgctggcgc cgtatctgaa
     3961 cgcatcgcaa ctggcgataa gcatcgtcgc gggtattcgc atggaggaca tgtcgcgctg
     4021 gctgaacggc catgcccgca tcgtacgcgt gatgccgaat acgccggcgc tgatcggcat
     4081 gggcgtaacg ggcctcgtcg cgaccggcag cgttgacgaa accgggcgcg cgctcgcgtc
     4141 gcaggtgctg ggcgcagtcg gcgaaaccgt ctggttcgac gacgaagcga agatcgacgc
     4201 cgtcaccgcg atctcgggca gtggtcccgc ttacgtgttt tatttcatcg aagcgttgca
     4261 ggaagccgcg cgccaactcg gcatggatga agcgcaaggc cgcgcgctgg cggtcgcgac
     4321 cttcaccggc gcggcgcaat tggccgcgaa ttcggatgag ccgccgagcc tgttgcgcga
     4381 acgtgtgacg tcgaagggcg gcaccacggc ggccgcgctg gcgtcgttcg aggcgaacgg
     4441 gatcaaggac gcgatcgtgc gcggggcgct cgccgccgat gcgcgcgccc gggaaatggg
     4501 cgacgagttc ggcaagttgt aagcgcgagt ggcgggcggc ggttggggtt tgcgcggaag
     4561 aattaaaacg aagccaacgc gtaatgcgcg gcaataccga caaacaacgc gccgcccagc
     4621 cagttgttat ggcggaacgc cgcgaagcac gccatccgtt cgcggttgcg gatcaacgtg
     4681 tagtgataga tcgcgcagcc cactgctgcc gcccatccca accagtacaa cacgccaaaa
     4741 ccgagcatca cgccgatgcc gacgtaaatc cccaacgtca ccgcatagca aagcatgatt
     4801 gccgccacgt cgaagcggcc gaaagtcagc gccgaagtgc ggatgccgat cttgatgtcg
     4861 tcgtcgcgat cgaccatcgc gtattcggta tcgtacgcaa ccgaccagaa cacgttggcc
     4921 agcagcatca cccaggcgag catcggcacg tgatcctgaa tcgcggcaaa cgccatcgga
     4981 atgccgaaac cgaacgcgat gcccagataa gcctgcggaa tcgcaaagaa gcgcttggtg
     5041 aacggatatg acccggcgac gaacaacgcc gccaccgaca attccttcgt cagcgtattc
     5101 agcggcaaga tcagcagaaa cgccagcagc gacaaaccgg ccgccagtgc caccgcttcc
     5161 caggccttga tcttgcccga cgtgatcggc cggttttccg tgcgcttcac gtagcgatcg
     5221 aagtcgcggt ccgcgtagtc gttgatcgcg caacccgccg aacgcatcag cacggtgccc
     5281 accgtgaaga tcaccagcag cggccacgac ggatgaccgt cggacgcaat ccacagcgcg
     5341 ttgagcgtcg gccatagcag aagcaggctg ccgatcggct tgtccatgcg gacgaggcgc
     5401 agatacagag gaagtcgggc gaacatggcg gattcggtga agtgcagacg gtgccgctat
     5461 tttacgggat gtgggcggcg ggcagcctgc tgggcaggcg aggctcgaaa agcttaactt
     5521 gcaaatcgaa aacgatgccg acgctgatgc tgagtccgaa gccgaagccc ccattaagcg
     5581 tgcaaaccgg tgccgcaaaa aacaaaacct cccgcgaagg ggaggctttg tttgtatttc
     5641 agttcaacgc aaatgccgat gcgctgcgtt gaacccaggc gaaacgccca gaacgcttaa
     5701 gccagcatcg agcgcagcat ccatgccgtc ttttcgtgcg tctgcatgcg ttgcgtcagc
     5761 aggtcggcgg tcggctcgtc gttggccgct tccgtcgacg ggaaaatttc gcgtgcggta
     5821 cgcaccacgg cttcctgacc ttcgaccagc tggcgaatca tttcttccgc agccggcaca
     5881 ccgtccgctt ccggaatcga cgacagcttc gcgaaatcct tgtaggtgcc cggcgcatgc
     5941 acgcccagcg cgcggatgcg ttcagcgatc gaatcgaccg cgagcgccag ctcgttgtac
     6001 tgcgtctcga acatcaagtg cagcgtgttg aacatcggac ccgtcacgtt ccagtggaag
     6061 ttatgggtct tgaggtacag cgtgtaggtg tcggcgagca gtcgcgacag accttctgca
     6121 atcttcttgc gatccttgtc gttgataccg atattgacgt gctgtacagc ttctttcttg
     6181 gccatgatga ctcctttgaa gagtgatgcg aaccggtcgg taaagtgacg atgtgcggta
     6241 ccaaacccaa catatcgaac gctcgacagt ttagcttaga gcagttgcgt tcgtgtgacg
     6301 cgtgcccgct tgtcgcaccc gggagctcgg ctcgatcacg cgccgagtgc gtgttgcagg
     6361 gcttgcgcca cgatcaccgc gagtccgctc gagacgatcg cgaatcccac tgccttgcgc
     6421 aacatcaatg cttgctgctg ccgtgcgttg cgtgcggcag gcgagccgcc gatggtggac
     6481 atcatcatct ggatcatggt cgtgcttcct cgattcgtat cgtttccggc gggacgccga
     6541 tgcgccccgc cctgctgcat gacttcctgc agacttcaac tgcttacttc ttgccgccct
     6601 cagcaaggtc cgccagcgcg gcgatcacac cttccgcgta ggccggatcg gcacggcgga
     6661 aatgctcgat ctgacgcgca acgatctcct gcggcacacc gttgatatgg cgcgcgatgt
     6721 tgccgaacag acgctgacgt tgcgccgcat cgaagagcgc aaacaacata cccggctgcg
     6781 tgtagtagtc cccgtcctgg cgatggtcgt aacgatcgac cgtccccgct gcaagcggcg
     6841 gctccgacgc gttgctgtcc tgcgcgaagt cgctgaaacg gttcggctcg tagttcacgt
     6901 tgccacccag gttgccgtcc gtgcgcatcg caccgtcgcg atggaacgaa tgcgggctcg
     6961 gtacacgcgg cgcattcacc gggatctgat ggtgattgat gccgaggcga tagcgctgcg
     7021 tatcgccgta cgagaacaaa cggccttgca acagacggtc cggcgagaag ccaatacccg
     7081 gcacgacatt ggccggcgtg aacgccgcct gctccacatc cgcgaagtag ttcgccgcgt
     7141 tgcgattcaa ctcgatcgtg ccgacgtcga tcagcgggta atccttctgc gaccacacct
     7201 tcgtgacgtc gaacggattg aagcgatagg ccgcggcgtc cgcttccggc atcacctgga
     7261 tggcaaaacg ccatgccggg aagttgcctg cttcgatatt cgtgaacaga tcgtgctgcg
     7321 cgctttcacg gttctgtgcc accacttgcg ccgcttcggc atcggtaaag ttctcaacgc
     7381 cttgcatcga cttgaagtga aacttcaccc agaaacgctc gttgttcgcg ttgatgaacg
     7441 aatacgtgtg cgagccgaag ccgtgcatct ggcggtagct ctgcggaata ccgcgatcgc
     7501 tcatcagaat ggtgacctga tgcaacgact ccggatgacg cgaccagaaa tcccatgccg
     7561 cgatattgct gcgcatgttg gtgtaagggt cacgcttttg cgtgtggatg aagtccggaa
     7621 acttcagcgg atcgcgaatg aagaacaccg gcgtgttgtt gccgaccacg tcccagttgc
     7681 cttcttccgt gtagaacttg atcgagaaac cgcgcacgtc gcgttccgcg tcggcggcgc
     7741 cacgttcgcc ggccaccgtc gaaaagcgca tgaagagcgg cgtgtccttg ccgacctggg
     7801 cgaatacttt cgccttcgta tagcgcgaga tgtcgtgcgt gactttcagt gtgccgaatg
     7861 cgcccgaacc cttggcgtga acgcggcgtt ccggaatcac ttcgcggtcg aagtgagcga
     7921 gtttttcgag cagccagaca tcttgcagga cgaccggacc gcgcgcgccg gcggtcatcg
     7981 aattctggtt gtcggcgacg ggtgcgccgg ctgcattggt gagattacgt tcggacatgc
     8041 gtgactccag tggggctttt atcaaaaaca gagtacaggt ataggtacgg cgcgctgatg
     8101 cctacggctt acgcagacaa ggcccgatcg accagcagag acaatgcggc cgtgcgcagg
     8161 ctgcgcgccg aggcggcaaa gctgtcggta atagcacggg cggattggac ggcgacggtc
     8221 aggactggct gcgagttcga ttgcgtcatg atttcctccg ggtctgatgg gatcgggtga
     8281 tccatggagg aaactataac aaaactatca gatcattgtt gttgattaat tttatctatt
     8341 caataggaat tggggctttc gaccctcgag aggttggccc gccaggccgc tgcgtaacac
     8401 cgacgaggcg gaattgagtg aggaacggcc gccaactgcc gcaggacgtg gactcagttg
     8461 acggcgaccg gcagatcgag ctttttgacg cctgtcagat cgcaggcgct gatggcatcg
     8521 ctgatcgctt cgatagcggg catgcgcgtg aagctcttgc gccaggcgag cacgacacgg
     8581 cgatccggca ccggctcgcc gaagggcacg tagctgagca ggcccgcgtc gataccgccg
     8641 gcgtgcggct tgacctcatt caccgacatg cgcggcagca cggtgatccc gacaccgctc
     8701 gccaccatat gacgaatggt ttccagcgac gaaccttcga aagtcttctg aataccgtcc
     8761 gcgttctgcg agaaacgcat caattcaggg cacacgccca gcacgtggtc gcggaagcag
     8821 tggccgctgc cgagcagcag catggtttcc tgcttgaggt cctcggcgtc gatcttcgcg
     8881 cggttttccc acgcgtgacc ggacggcagc gcgacgacga acggttcgtc atagagcggg
     8941 cgcagcatca gacccgtttc cgggaacggc agcgccatga cggcgacgtc gatttcgcct
     9001 tgtttgagca gttcgatcag cttgatcgtg taattttcct gcagcatcaa cggcatctgc
     9061 gggacacgct tgatcatctg tttgacgagt gtaggcagta gatacggccc gatcgtatag
     9121 atgacgccga ggcgcaacgg cccgaccagc gggtctttgc cctgtttggc gatctccttg
     9181 atcgcgagcg tttgttcgag gacgcgctgg gcttgcgtga cgatctgttc gccgatcggc
     9241 gtgacactga cttcgctggt gccgcgttcg aaaatctgga cgttgagctc gtcttcgagc
     9301 tttttgatgg ccaccgacag ggtcggctgg ctgacgaaac acgcttcggc ggcccggccg
     9361 aagtggcgct cgcgggcaac cgcgacgatg tatttcaatt cggtgagcgt cattagggga
     9421 tcaatcagtg cggtgagttc gataggttct agttatacac ccatagggtc ggttcgccaa
     9481 cctggttcac cgacctttta cgctcaagcc ttcagatact gttcgcgagc gccgagccag
     9541 cgggtcagat gctgcgtaac gacctcggga tacttctcca gcagggccgc ggccgcttcc
     9601 cgcgccgggt cgatcagcca ctggtcgg
//
LOCUS       DATASH010000049         3503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3961929,
            whole genome shotgun sequence.
ACCESSION   DATASH010000049 DATASH010000000
VERSION     DATASH010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3961929"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(294..1517)
                     /locus_tag="VHV99_01620"
     CDS             complement(294..1517)
                     /locus_tag="VHV99_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008924359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX3634187.1"
                     /translation="MSESDLPVPAIPNARDAVRALRSSQIREVANAGFGVADVLPFWF
                     GESDRVTPAFIRDAASAALAAGDTFYTHNLGIAPLRAALAGYVSELHGTTSPDHIAVT
                     SAGVNALMLAAQLVVGAGDRVVAVTPLWPNLVEIPKILGAHVETVALGYGERGWQLDL
                     EQLLAALTPDTKMLLINSPNNPTGWVMSRDEQRAVLEHCRRHGIWIVADEVYERLYYA
                     DAEPGARGAHSRTAPSFLDLAARDERVICVNSFSKAWLMTGWRLGWIVAPVSLMDDLG
                     KLVEYNTSCAPAFVQQAGIAAVQHGERFTQELVLDLKASRDHLVRSLSAVPGVDVKAP
                     PGAMYLFFSMPRASRSLELCKAMVREVGLGVAPGSAFGPQGEGFVRWCYACDTARLDV
                     GVERLKRFLAQHGLH"
     gene            complement(1925..2827)
                     /locus_tag="VHV99_01625"
     CDS             complement(1925..2827)
                     /locus_tag="VHV99_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006764495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX3634188.1"
                     /translation="MNVTLRQLRVFIEVARLQSFSRAGDEIGLTQSAVSRCVRELEGE
                     IGLKLIDRTTREVQLTDVGGNLVSSVSRLLTDLDDALREIREIGEQRRGRVVVAASPT
                     IACRLMPGVVASCGRQFPYITLGLRDDVQSDVVRKVKSGEVDFCVIIGPLSADDLLSE
                     SLMTDSFCVVSRDDHPLAAQRQVAWTDLEGQQLVMLDRASGSRPIIDAVMQEYGVSAT
                     VVQELGHSATVFGLVEAGVGVSVLPWLALPLPAGASLVARPLVPRAERTVELVRRRDR
                     SLSPAAEAVWSLIRQLPARAEDLD"
     gene            3232..>3503
                     /locus_tag="VHV99_01630"
     CDS             3232..>3503
                     /locus_tag="VHV99_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004538461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX3634189.1"
                     /translation="MMLFDDLRDNEWALVEALFCAEPARSERRGRPRVEARAVVNAVL
                     WVLSTGEGWSKLPGRYPSPPTCRRRFDEWQADGTLAEIVKRLGTSGR"
BASE COUNT          717 a         1159 c         1053 g          574 t
ORIGIN      
        1 tcgcccgatt ctgatcggtc atattgatgg gagcacgcac cggggggagg cgaacaggca
       61 atcgagccaa acccagccgg acggagcagc gatattcctg aaaggaatcg cgtgactgaa
      121 cgccatatta cgcaaaaaca accagtgcgt aaagatgaaa tccccagcgc atttttctgc
      181 tttgccccag ttcgcccgct taaaaggcaa ggctggcgga atcgaacggg aatcggagcg
      241 gaaccggacg ttaaaccagg accttaaacc gctgccgcac gcgccgccgc cattcaatgc
      301 aggccgtgtt gcgccagaaa ccgcttcaga cgctcgacac ccacgtcgag ccgcgccgtg
      361 tcgcaggcgt agcaccagcg cacgaagccc tcgccttgcg ggccgaatgc gctgccgggc
      421 gccacgccca ggcccacctc gcgcaccatg gccttgcata gttcgagact gcgtgacgca
      481 cgcggcatcg agaaaaacag gtacatggcc ccgggcgggg cctttacgtc gacacccggc
      541 acggcggata gcgagcgcac cagatggtcg cgcgacgctt tcagatcgag caccagctcc
      601 tgggtgaagc gctcgccgtg ctggacagcc gcgatacccg cctgctggac gaaagccggc
      661 gcgcacgacg tgttgtattc gaccagcttg cccagatcgt ccatcagcga gaccggtgcg
      721 acgatccagc cgagccgcca gccagtcatc agccacgcct tggaaaacga gttcacgcag
      781 atcacgcgct cgtcgcgcgc ggccagatcg agaaacgacg gcgccgtgcg cgaatgggcg
      841 ccgcgcgcgc cgggttctgc gtccgcgtaa taaagacgct cgtagacttc gtcggcgacg
      901 atccagatac cgtggcggcg gcaatgctcc agcacggcac gctgctcgtc gcggctcatc
      961 acccagccgg tcggattgtt cggcgaattg atcagcagca tcttcgtatc cggcgtcagc
     1021 gcggccagca actgttccag atcgagttgc catccgcgtt caccgtagcc cagcgcgacg
     1081 gtttccacat gcgcgcccag tatcttggga atttcgacca gattcggcca cagcggcgtt
     1141 actgcgacaa cacggtcgcc cgcgcccact accagttgcg cggccagcat caatgcattg
     1201 acaccggcgc tcgtcaccgc gatgtgatcg ggcgaggtcg tcccgtgcaa ctcgctcacg
     1261 tagcctgcga gcgccgcgcg cagcggcgca atgcccaggt tatgcgtata gaaggtgtcg
     1321 ccggccgcca acgccgcact ggcggcgtcg cggatgaacg ccggggtcac ccggtcggac
     1381 tcgccaaacc agaacggcag cacgtctgca acgccgaaac cggcattcgc gacttcacgg
     1441 atctgcgaag aacgcagtgc gcgcacggcg tcgcgcgcgt tgggaatggc gggcacaggc
     1501 aaatccgatt cgctcatcga agcttccaga cgggtaatgg ataaaaagct gacgcttgaa
     1561 agaggcgaca gtttagcgca gcgcgtgttt ttgaaactat gaagtcactg actccgcaag
     1621 gtgcgatcag aagctggccg tttcagcgga tctgattaac gggcgaggct caaggcagtg
     1681 agcgcttcag tggatccgat caacgggcga ggctcgaagc agtggccgct ccagcggacc
     1741 cggccaacag gtgaggctct cacggcagtg agcgctccaa cggacccaac caaccggcga
     1801 agctcaaagc agtgaccgct ccaacgcacc cgaccaacag gccaggctca aggcagtacc
     1861 gctctgcaaa gcgcaaccaa tccgacctcg cagaaccgac ggcccgtgcc cctgccccga
     1921 gtcatcagtc caaatcctcc gcccgtgcgg gcaattgacg aatcaggctc cacactgcct
     1981 ccgccgcggg cgacaacgag cgatcgcgcc gccgcaccag ttcgaccgta cgctcggcgc
     2041 gcggcacgag cggccgtgcc accagcgagg cgcccgccgg cagcggcagc gccagccagg
     2101 gcagcacgct cacgccgaca ccggcctcga ccagcccaaa aacggtcgcg gaatgcccta
     2161 gctcctgcac cacggtggcg cttacgccgt actcctgcat cacggcgtcg atgatcggcc
     2221 ggctgcctga cgcgcgatcg agcatgacga gttgctggcc ttcgaggtcc gtccatgcca
     2281 cctgccgctg cgccgccaag ggatggtcgt cgcgcgaaac cacgcaaaac gaatccgtca
     2341 tcagcgattc gctgagcagg tcgtcggcgg ataacgggcc gatgatcacg caaaaatcga
     2401 cttcgcccga cttcaccttg cgcaccacgt cgctttgcac gtcgtcgcgc agacccaagg
     2461 tgatgtaggg gaactggcga ccgcacgacg ccaccacgcc aggcatcaac cggcaggcaa
     2521 tggtcgggct cgcggccacg accacacgcc cgcggcgctg ctcgccgatt tcgcggatct
     2581 cgcgcagcgc gtcgtccaga tctgtcaaca ggcgcgacac gctcgacacc aggttgccgc
     2641 cgacatccgt gagctgcact tcccgcgtgg tgcgatcgat cagtttcaaa ccgatctccc
     2701 cttccagttc gcgtacgcag cgactcaccg ccgattgcgt cagaccgatc tcatcgccgg
     2761 cccggctgaa gctctgcaaa cgggccacct caatgaaaac ccttagctga cgcagtgtca
     2821 cattcatctg caaacctcat caattacctc atttgatgcg cattgtatgg ccaaatgtcg
     2881 caccatagcg gttttcgcga cttcgctgca atgcagtaat aaggcgcaaa cgcacgctgg
     2941 ccgggcttga ttgcacaaga ttggtgattg tcccgatttg cgagatgggt tttttggatt
     3001 ctcatacgcc cctagctagc gcccgcgctg gcccgctgtc acagggcttt caggtagtcg
     3061 gactcaactt ggcacacttc ctgcatttac atgcacacag gatcggcgcc atgacttctg
     3121 actgaatagc agatcgtggc aagcccgacc cccttggcac tcgcctatcg agtgcatgga
     3181 gagtgcgcgg cgcggggcag cgcaccggag ttagcttcgc tgaggtgaaa catgatgctg
     3241 ttcgacgatt tgagagacaa cgagtgggcg ctggtcgagg cgttgttctg tgcggagcca
     3301 gcacggagcg aacggcgcgg tcggccacgc gtggaagcac gcgcggttgt caacgctgtg
     3361 ctttgggtgc tgtcgacggg cgaaggctgg tccaagctgc cgggccgcta tccgtcgccg
     3421 ccgacttgcc gccgtcgctt cgacgaatgg caagccgatg gtacgctcgc cgaaatagtt
     3481 aagcgactcg gcacgagcgg ccg
//
LOCUS       DATASH010000050         4208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2577691,
            whole genome shotgun sequence.
ACCESSION   DATASH010000050 DATASH010000000
VERSION     DATASH010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2577691"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="VHV99_01635"
     CDS             <1..613
                     /locus_tag="VHV99_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HEX3634190.1"
                     /translation="DRQLHSSGHWLLRLPQEDFCQVYGVPSHRKYENEGGPGVLDLAR
                     ILHQSVSAQQDIETLLASQILFWMLAAPDGHAKNFSIRLLAGGQYRLTPLYDVMSIWP
                     VEGNGPNQWSWYKARLAMAMWSRSKHDAFRDVQRRHFNTMALRCSYGANAEPLIQRLI
                     EQTPEVIARVSAELPERFPGKVAERIFKGLKSSAARLDKMSAA"
     gene            933..1787
                     /locus_tag="VHV99_01640"
     CDS             933..1787
                     /locus_tag="VHV99_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3634191.1"
                     /translation="MTNETHSPDSIAVAERVRELMSRHGIGKRQQTTELCRILDLSFS
                     QGHRKLRGNSPWTLSQIKKVAEVFGEPAAQLFGAQSLDPGMVGAIAQEAVFSIGAIEL
                     ACTAWVGAALEPGSRPEFVAYSKLGQWRVARHDGTLYQSAYEVHKIEIYPRRAETDKP
                     TIAVVDDDQASADNLRDYLERSGFAAVAIYGLAAFAEQLQTQVFDGIVIDWLFGSQTS
                     AAAIGAVRASENPDAPIFVLTGELLTGKASESEISQVIRNYDIACYEKPARMAILVAD
                     LSKRLNRP"
     gene            complement(1903..3762)
                     /locus_tag="VHV99_01645"
     CDS             complement(1903..3762)
                     /locus_tag="VHV99_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006053221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3634192.1"
                     /translation="MADQGTHRQITRRPWRRALYVLIWLTALAAPAGAIGYLLYANLL
                     NPRATEQLTGSYDGFYWDAAQLQIAYARFENQLLLYQSGVDDDYQRLMLRYQLLQSKL
                     HVMAGSTHRLTTQLVLLQRQLEEIQSLTHLLDGIEPEVDALPKNRSRANQIVMELRQH
                     WSEVNDLALSRRFADVADREAMSRDFIDKRRMLFAGGLLLLLLSAAATTLLVLNGRRR
                     TRLMHQQHAALEAEHQAGRAAREASLAKDAFLGMISHELRTPLHAIVSSIELLGFNYH
                     SEADRKVIQRLETAGRHLEAQMKDLTDYARLGAGKLELRHEHFEARELLASIVDEHAM
                     AAAARGLTLASEASGMSGLVDSDPHRIRQIVNNLVTNAIRYTETGTVRVQLRQRPAVL
                     IFVVSDTGPGVPHAQIPLIFQEFTQLDASRTRRFEGAGMGLTIVQGLVRLFGGTVEVA
                     STVGKGTTFTVTIPVTPVAATEPPGVAAHAEPGGARPCVLIVDDNRLVRESLGEMIAH
                     MDFDAHAVASADEALAWLDAHRCDVVLLDLHMPDRDGYTFLADFTARGGPSSGVPVIV
                     VSAYAPEADSAGRADGGAAEAGLQPFFETLLKPVHYEELRSALQRALALRHTA"
     gene            complement(3732..>4208)
                     /locus_tag="VHV99_01650"
     CDS             complement(3732..>4208)
                     /locus_tag="VHV99_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX3634193.1"
                     /translation="GLLLALAAWSMQAQAESLPLSLDVTGKISNTNDAAHQSYHFTEA
                     QLLALPAHSITTATTWTPRSTFTGPLLADILKTVGASGSAVEIHTLDDYTYTIPVSDC
                     DRYGVVVAYSMNGRRLKISDFGPLFLIYPRDAFPDELTGALGDSKFVWQIKALIVK"
BASE COUNT          796 a         1380 c         1309 g          723 t
ORIGIN      
        1 cgaccggcaa ttgcattcga gcgggcactg gctgctgcgc ctgccgcagg aagacttctg
       61 ccaggtgtac ggcgtgccgt cgcatcgcaa gtacgaaaac gaaggcggtc cgggcgtgct
      121 ggacctcgcg cggattcttc accaatcggt ctcggcgcaa caggacatcg aaacgctgct
      181 ggcaagccag atccttttct ggatgctggc cgcgccggac ggccacgcca agaacttcag
      241 cattcgcctg ctggcgggcg gccagtaccg cctgacgccg ctttacgacg tgatgtcgat
      301 ctggccggtg gaaggcaatg ggccgaacca gtggtcgtgg tacaaggcca ggctcgccat
      361 ggcgatgtgg tcgcgcagca aacacgacgc ctttcgcgac gtgcagcggc gccacttcaa
      421 caccatggcg ctgaggtgct cgtacggcgc gaacgccgag ccgttgatcc agcggctgat
      481 cgagcagacg cccgaggtca tcgcgcgggt ctcggccgaa ttgcccgagc ggtttccggg
      541 caaggtcgcc gagcggatct tcaaaggtct gaaaagctcg gcggccaggc tcgacaagat
      601 gtcagccgca taagcaggca gccgcaaacg tagtcatgtc atatcaagcc gcctgagacg
      661 ctcaataatt ctctaccgat gcggtacggt ttcatcggta aaatgcaatt catgccaagt
      721 gcataaaaaa atcgccgggt ccatccgctc gcgccgcgca ctgaaagacc cggccacggt
      781 catacaaaaa cacaaaccgc gatacttttt acggtacatt tgctgtcccc cgcgccgccg
      841 cgtcaacgca cgttcaatgt gcatggccgc gccgcgcgtg cggtatcgca ctctccacag
      901 ccacccttca agaaggcgga tctttacgca gtatgaccaa cgaaacacac tcacctgact
      961 ccatcgcagt cgccgagcgc gtgcgcgagt tgatgagccg gcacggcatt ggcaaacgcc
     1021 agcaaaccac ggagttgtgc cgcatcctcg atctgagttt ttcgcaggga caccgcaagc
     1081 tgcgcggcaa cagcccgtgg accctctcgc agatcaagaa ggtggccgaa gtgttcggcg
     1141 aacctgccgc tcagctattc ggcgcgcagt cgctcgatcc gggcatggtc ggtgcgatcg
     1201 cccaggaggc ggtgttcagc atcggtgcga tcgagctggc ctgcaccgca tgggtcggcg
     1261 ccgcgctcga accgggcagc cgcccggaat ttgtcgcgta ctcgaagctc ggccagtggc
     1321 gcgtggcgcg tcacgacggc acgctttacc aaagcgcgta cgaggtccac aagatcgaga
     1381 tctatccgcg ccgcgctgaa accgacaagc ccaccatcgc cgtggtcgac gacgatcaag
     1441 cctccgccga caacctgcgc gactatctcg agcgcagcgg tttcgcggca gtggcgatct
     1501 acggcctcgc ggcgttcgcc gaacaactgc aaacccaggt cttcgacggc atcgtgatcg
     1561 actggctgtt cggctcgcaa acctcggcgg cggcgatcgg cgccgtgcgg gcatcggaga
     1621 atccggacgc gcccattttc gtgctgaccg gcgaactgct gaccggcaag gccagcgaat
     1681 cggagatcag ccaggtgatc cgcaattacg acatcgcgtg ctatgaaaag cccgcacgca
     1741 tggcgatcct ggtcgccgat ctgtccaagc gcctcaaccg gccctgaggc gcggcaagca
     1801 tctgaaggtg tgtgctcggg ctgagccctc ctccgtgcgc gcctcccccg gccgtcgcat
     1861 cacgcgtcat catcgtcatc acgcgccgca tcaggcatcg catcacgccg tatgccgcaa
     1921 agccaaggca cgctgcaacg cgctgcgcag ttcctcgtaa tggactggct tcaataaggt
     1981 ttcaaaaaac ggctgcaagc cggcttctgc cgcgccccca tccgcacggc ccgcgctatc
     2041 cgcttccggc gcgtacgcgc tcaccacgat caccggcaca cccgacgacg gtcccccacg
     2101 tgccgtgaaa tccgcgagaa acgtataacc gtcgcgatcc ggcatatgca gatcgagcaa
     2161 caccacgtca caacgatgcg cgtcgagcca cgcgagcgcc tcgtcggcgc tggcgacggc
     2221 atgcgcgtcg aaatccatgt gcgcgatcat ttcgccgagc gactcgcgaa ccaggcggtt
     2281 gtcgtcgacg atcagcacgc acgggcgcgc gccgcccggt tccgcgtgtg ccgccacgcc
     2341 gggcggctcg gttgccgcga ccggcgtgac cgggatcgtc accgtgaagg tggtgccctt
     2401 gcccaccgtg ctcgccacct cgacggtgcc gccgaacagc cgaaccagcc cctgcacgat
     2461 cgtgagcccc atccccgcgc cctcgaagcg gcgcgtacgc gacgcgtcca gttgcgtaaa
     2521 ctcctgaaag atcagcggaa tctgtgcatg cggcacgccc ggccccgtat cgctgacgac
     2581 gaagatcagc accgctggcc gctgcctgag ttgcacgcgc acggtgccgg tttcggtata
     2641 gcggatcgcg ttggtgacca ggttgttgac gatctggcga atccggtggg gatcggaatc
     2701 gacgaggccg ctcatgccgc ttgcctcgct cgccagcgtg aggccacgcg ccgcggctgc
     2761 catcgcgtgt tcatcgacga tcgacgccag cagttcgcgc gcctcgaaat gctcgtgacg
     2821 caactcgagc ttgccggccc cgaggcgcgc gtagtcggtc aggtctttca tctgggcttc
     2881 cagatgccgc ccggcggttt cgagccgctg gatcaccttg cggtcagcct cggagtggta
     2941 gttgaagccc agcaattcga tcgacgacac aatcgcgtgc aacggcgtgc gcagctcgtg
     3001 gctgatcatg ccgaggaacg cgtccttggc gaggctcgcc tcgcgcgccg cgcggcccgc
     3061 ctggtgttcg gcttcgagcg ccgcatgctg ctgatgcatc aggcgcgtgc ggcgccggcc
     3121 attgagcacc agcagcgtgg tcgcggccgc cgacagtaac agcagcaaca ggccgcccgc
     3181 gaacagcatg cggcgcttgt cgatgaaatc gcggctcatc gcctcacgat cggccacgtc
     3241 ggcgaaacgg cggctcaacg cgagatcgtt gacctcgctc caatgctggc gcagctccat
     3301 gacgatctga ttggcgcggc tgcggttctt cggcaacgcg tcgacctcgg gctcgatccc
     3361 gtccagcaga tgggtgagcg actggatttc ctccagctgc cgttgcagca atacgagctg
     3421 cgtggtcaaa cggtgcgtcg aaccggccat cacatgcagc ttcgactgca gcaactggta
     3481 gcgcagcatg agccgctgat agtcgtcgtc cacgccggac tgatacagca gcagctgatt
     3541 ctcgaaacgc gcgtaggcga tctgcagctg cgcggcatcc caatagaagc cgtcgtatga
     3601 accggtcagt tgttcggtgg cgcgcgggtt gagcaggttc gcgtacaaca gatagccaat
     3661 cgcgccggcc ggcgccgcga gcgcggtcag ccagatcagc acatagagcg cacgacgcca
     3721 cgggcggcgc gttatttgac gatgagtgcc ttgatctgcc atacgaattt cgaatcgccc
     3781 aacgcgcccg tgagttcatc agggaatgcg tcgcgcggat agataagaaa cagcggcccg
     3841 aagtcgctga tcttcaggcg ccggccgttc atgctgtacg caacgaccac gccgtagcga
     3901 tcgcagtccg acactggaat ggtgtacgta tagtcgtcga gcgtatggat ctcgactgcg
     3961 ctgccggatg cgccgactgt cttcaggata tccgcgagca gcggtcctgt gaaggtcgaa
     4021 cgcggggtcc aggtggtggc ggtcgtaatc gaatgggccg gcagcgcgag cagttgtgcc
     4081 tcggtgaagt gatacgactg atgcgccgcg tcgttggtgt tgctgatttt gcccgtcaca
     4141 tcgagcgata acggcaacga ctctgcctgc gcctgcatgc tccacgcagc gagcgcgagg
     4201 agcagacc
//
LOCUS       DATASH010000051         3706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6731565,
            whole genome shotgun sequence.
ACCESSION   DATASH010000051 DATASH010000000
VERSION     DATASH010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3706
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6731565"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..958
                     /gene="tsf"
                     /locus_tag="VHV99_01655"
     CDS             77..958
                     /gene="tsf"
                     /locus_tag="VHV99_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011352460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX3634194.1"
                     /translation="MAAITASMVAELRAKTDAPMMECKKALTEAEGDMARAEELLRVK
                     LGNKASKAASRVTAEGVVASFIGGNAGSLVELNCETDFVSKNDDFLAFSKTVAELVAT
                     QNPADVAALSALPLDGSTVDAVRLALVGKIGENLSIRRFVRFETSNKLAAYLHGTRIG
                     VLVEYTGADEQVGKDVAMHIAAMKPVSLSSDDVPADLIAKERSIAEQKAAESGKPAEI
                     VAKMVDGSVQKYLKEVSLLNQTFVKNDKQTIEQMLKAGNASVQKFALFVVGEGIEKKQ
                     DDFAAEVAAQVAAAKQQ"
     gene            1178..1891
                     /gene="pyrH"
                     /locus_tag="VHV99_01660"
     CDS             1178..1891
                     /gene="pyrH"
                     /locus_tag="VHV99_01660"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008652269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX3634195.1"
                     /translation="MPTAYKRVLLKLSGEALMGDDAFGINRATIERMVADVAEVVRLG
                     TQLAVVIGGGNIFRGVAGGAAGMDRATADYMGMLATMMNALALQDAMRHAGIEARVQS
                     ALRMDQVVEPYIRPRAIRQLEEGKVVIFAAGTGNPFFTTDTAAALRGSEVGAEVVLKA
                     TKVDGVYSADPKKDPSATRYTTISFDEAIGRNLQVMDATAFALCRDQKLPIRVFSIVK
                     PGALKRIVQGEDEGTLVHV"
     gene            1992..2552
                     /gene="frr"
                     /locus_tag="VHV99_01665"
     CDS             1992..2552
                     /gene="frr"
                     /locus_tag="VHV99_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX3634196.1"
                     /translation="MSVADIRKGAEQKMQRSIDAFKNDLSKIRTGRAHTGLLDHIQCD
                     YYGSLVPISQVANLTLIDARTIGVQPWEKKMVKVVEKAIRESDLGLNPATQGDVIRVP
                     MPALTEERRRELTKVVKSEAETAKVAVRNLRRDANEQLKKLVKDKEISEDDERRAGDD
                     VQKLTDKFVTEIDKLVVTKEAEIMTV"
     gene            2851..3633
                     /gene="uppS"
                     /locus_tag="VHV99_01670"
     CDS             2851..3633
                     /gene="uppS"
                     /locus_tag="VHV99_01670"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010092065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEX3634197.1"
                     /translation="MTYTSSTVRVPDVAAVPRHIAIIMDGNGRWATQRRLPRVAGHTR
                     GVDAVRAAVEACARQGVEYLTLFAFSSENWRRPNDEVSFLMRLFVTALEREIGKLHAN
                     GIRLRVVGDLSRFDTRIRDLIKRAETKTARNTRLTLTIAANYGGRWDIMQATRRLAEQ
                     SALAGKAVEVNEDSFAEHLSMAYAPEPDLFIRTGGERRVSNFLLWQLAYTEFYFTDTF
                     WPDFDAEALGHAIASYAERERRFGRTSAQLEPQSQNVDSLPC"
     gene            3627..>3706
                     /locus_tag="VHV99_01675"
     CDS             3627..>3706
                     /locus_tag="VHV99_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018435520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX3634198.1"
                     /translation="MLKTRVITAIILLAIFLPVTLFAPVGA"
BASE COUNT          755 a         1113 c         1174 g          664 t
ORIGIN      
        1 cgacgttgaa acgaattctt gccgccggca tcgaagtccg ggcggcgtgt gacagatgga
       61 actcaaggag caaatgatgg cggcaattac cgcaagcatg gttgcagaac tgcgcgcaaa
      121 gaccgacgcg ccgatgatgg agtgcaagaa ggcgctgacg gaagccgaag gcgacatggc
      181 gcgcgctgaa gagctgctgc gcgtgaagct gggtaacaag gccagcaagg cagcgtcgcg
      241 cgtgacggct gaaggcgtgg tcgcatcgtt catcggcggc aacgcgggtt cgctggtcga
      301 actgaactgc gaaaccgact tcgtttcgaa gaacgacgac ttcctggctt tctcgaagac
      361 cgtcgctgaa ctggtcgcta cgcaaaaccc ggctgacgtc gctgcgctgt cggcgctgcc
      421 gctggacggc tcgacggtcg acgcagtgcg tctggcgctg gtcggcaaga tcggtgaaaa
      481 cctgtcgatc cgccgtttcg tgcgtttcga aacctcgaac aagctggcag cgtacctgca
      541 cggcacgcgt atcggcgtgc tggtcgagta caccggcgcg gacgaacagg tcggcaagga
      601 cgtggcaatg cacatcgccg cgatgaagcc ggtttcgctg tcgtcggacg acgtcccggc
      661 ggatctgatc gccaaggaac gcagcattgc tgaacagaag gctgctgaat cgggcaagcc
      721 tgctgaaatc gtcgccaaga tggtcgacgg cagcgtgcag aagtacctga aggaagtgtc
      781 gctgctgaac cagacgttcg ttaagaacga caagcagacg atcgaacaga tgctgaaggc
      841 tggcaacgcg agcgtgcaga agttcgcgtt gttcgtggtt ggcgaaggca tcgagaagaa
      901 gcaggacgac ttcgcagcgg aagtggccgc tcaagtcgct gctgcaaagc agcagtaagc
      961 atcgcttaag ctttggttag taccgttgga cccgcggcgg agcaagactt tgccgcggtc
     1021 ggcagcgcca cagggctgcg cgcgggtaac cctcgtggcg cagccggctc cggcgaaccc
     1081 cagggttcgt caatttggcg acgcttgccc gaatcagtat ttcaccccta cattagtccc
     1141 ttgttgttgc cctgttctgc gcgatctgga tacccctatg cccactgcct ataaacgcgt
     1201 cctgctcaaa ctctccggtg aagctctaat gggcgacgat gcctttggca tcaatcgcgc
     1261 gaccatcgaa cgaatggtgg cggacgtggc cgaagtggtc cgtctcggaa cgcagctggc
     1321 cgtggtgatc ggcggcggta acattttccg cggcgtcgcg ggcggcgcgg ccggtatgga
     1381 tcgcgccacg gctgactaca tgggcatgct ggccaccatg atgaacgcgt tggcgctgca
     1441 ggacgcaatg cgccacgccg gtatcgaagc gcgcgtgcaa tccgcgctgc gcatggacca
     1501 ggtagtcgag ccttacatcc gtccgcgcgc gattcgccag ctcgaagaag gcaaagtcgt
     1561 gattttcgcg gccggcaccg gcaacccgtt cttcacgacc gatacggccg ccgcgctgcg
     1621 tggctcggaa gtcggcgcgg aagtcgtgct gaaagccacc aaggtggacg gcgtgtattc
     1681 ggccgacccc aagaaagacc cgagcgcgac ccgttacacc acgatcagtt tcgacgaagc
     1741 catcggccgc aatttgcagg tgatggacgc cacggcgttt gcgctgtgcc gcgaccagaa
     1801 actgccgatc cgggtgtttt cgatcgtcaa gccgggcgcg ctcaagcgca tcgtacaagg
     1861 cgaggacgag ggcaccctcg tccacgtgta aactctcgtt cacataacgt gggctttaac
     1921 gcgagcccag gccgccgcat aatgcgcgcg ctggccggct cgcaccgttt tgaaggttcg
     1981 gaggtttaaa aatgtctgtg gctgatatca ggaagggcgc tgaacaaaag atgcagcgtt
     2041 ccatcgacgc gttcaagaac gatctgtcga agatccgcac gggccgtgcg cacacgggcc
     2101 tgctcgatca catccagtgc gattactacg gttcgctggt gccgatttcg caggtcgcga
     2161 acctgacgtt gatcgacgca cgcacgatcg gcgtgcagcc ttgggaaaag aagatggtca
     2221 aggtcgtcga aaaggcgatc cgcgagtcgg acctcggtct gaacccggcg acgcaaggcg
     2281 acgtgatccg cgtgccgatg cccgcgttga ccgaagaacg ccgccgcgaa ctgaccaaag
     2341 tggtcaagag cgaagcggaa acggccaagg tcgctgttcg taacctgcgc cgtgatgcaa
     2401 acgagcaact gaaaaagctc gtgaaggaca aggaaatttc tgaagatgac gagcgccgtg
     2461 caggtgacga cgttcagaaa ctgacggaca agtttgtcac ggagatcgac aaacttgtag
     2521 taactaaaga agctgagatc atgacggtct gaggccgttg gctcccggga aaacgccgga
     2581 ccgcccaagg ttttccgctc cgcaggaatt cctctgcccc gaaggaagtc ccttttgggt
     2641 tgagggattg ccccctcagg ggcttcgcgc gaagcgccag gttttgggag cacccggcag
     2701 aacgccgggc cgcccgaagt tttccgctcc gcaagaagtc ctctcggggg attgcatcct
     2761 caggggagtc tggcgcgaag cgccagaatt gggggcactc agcacgttca agacttccac
     2821 ttcttttttg cagtcactgt cccgacggcc atgacctata ccagctcaac cgtgcgcgtg
     2881 cctgatgtcg ccgcggtgcc gcgccatatc gcgatcatca tggacggcaa cggccgttgg
     2941 gcgacccagc ggcgcctgcc gcgcgtggcc ggccatacgc gcggcgtcga cgcagtgcgc
     3001 gcggccgtcg aggcgtgcgc ccggcagggt gtcgaatacc tgacgctgtt tgccttcagt
     3061 tcggaaaact ggcgccgccc gaacgacgaa gtgtcgttcc tgatgcgtct gttcgtcacc
     3121 gcgctggagc gcgaaatcgg caagctgcac gcgaacggca tccgtttgcg cgtggtcggc
     3181 gatctgtccc gattcgatac gcggatccgc gatctgatca agcgcgcgga aaccaaaacc
     3241 gcgcgcaata cccgcctcac gctcaccatt gccgccaact acggcggccg ctgggacatc
     3301 atgcaggcca cccgcaggct cgccgagcaa tcggcgctgg cgggcaaggc ggtcgaggtg
     3361 aacgaggact cgttcgccga acacctgtcg atggcctatg cgccggagcc ggacctcttc
     3421 atccgcaccg gcggcgagcg ccgcgtcagc aactttctgc tgtggcaact cgcctacacc
     3481 gagttttact tcaccgacac attctggccg gatttcgacg cggaagcgct cggccacgcc
     3541 atcgcttcct atgcggagcg tgagcgccgc ttcggccgca ccagtgctca actcgagccg
     3601 caatcgcaaa acgtcgattc gcttccatgc taaaaacccg tgtcatcacg gcgatcatcc
     3661 tgctggcgat ctttctgccg gtcacgttgt tcgcgccggt gggcgc
//
LOCUS       DATASH010000052         3156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1391060,
            whole genome shotgun sequence.
ACCESSION   DATASH010000052 DATASH010000000
VERSION     DATASH010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1391060"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1716
                     /locus_tag="VHV99_01680"
     CDS             <1..1716
                     /locus_tag="VHV99_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HEX3634199.1"
                     /translation="AGVPVAEGSLAQDAAAASARAAEPEDGVSTREQIPVREASDASD
                     EAAAYGVALSAAGREGAAREVAEREAAAREVAERVAAEREAEIRAAAAREAAQREAAA
                     GEAAKRLAAEHAATEHTVGESTAAQQAAAERAEAERVAADQAAQERAAGGEQAAAREQ
                     AAAGQQAAAKEQAAEEHEASTDESHTAFPTQEPNASPPVWPESHAAPAPLEPAQPRAD
                     IEPAAQSGSTSEPLPSAHHENLDGAATAASLAAAAELGAEALPLTSLEPVSETFQQEA
                     PPHRLQWDDEPASSAPAAPLAESANESQTRFDTPAPVIDFTPHEAAPRTTSPFDQPSI
                     DTSAHPAPDTSVPAVPAVQQHTAHVDPAYKPSHQPQPQAQQQSHQQAETATPDGAPTL
                     EPAPVEQTQPARSIPSEFPRDAVDAFSSLNMPLPPRVESTDAAESAPESLSTQPVASP
                     EATAQQAVAPHDPDDTPHVSDEFSAGTAGHAAVAGLGAVGFGALKLDFDLELPPSPAQ
                     PLPAFTRADLSRIARNKLDLAAEYIDLGDLSGARLLINEVIEANDPATRTEARALLST
                     LAPLS"
     gene            1713..2525
                     /gene="truA"
                     /locus_tag="VHV99_01685"
     CDS             1713..2525
                     /gene="truA"
                     /locus_tag="VHV99_01685"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEX3634200.1"
                     /translation="MKRIALGVQYDGSAFCGWQSQPHGKTVQDELERALREFAQVPVQ
                     TVVAGRTDTGVHGLGQVVHFDTELDRTDFSWVRGTNAFLPKTIAVQWAKPMPDAFHAR
                     FSAFERTYYYVLYVHPFRSPMQATRAGWVHTPLDVDAMQAAAAHLIGEHDFSAFRSSQ
                     CQAKTPVKHLYQIDMRQQGDFVHFRFRANAFLHHMVRNLMGCLVDIGRGRHPAEWMAE
                     VLASRDRDCAAPTFMPDGLYLARVGYPEQFAVPAPQTGSVPWNTVWTEQAQT"
     gene            2522..>3156
                     /locus_tag="VHV99_01690"
     CDS             2522..>3156
                     /locus_tag="VHV99_01690"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEX3634201.1"
                     /translation="MKSTENLAAETDAAMPQQAAPRRTRIKLCGLSKPEDVAQAIDLG
                     ADAIGLVFYPPSPRSVSVAQAVELVHDVPPFVSVVGLFVNATHDWIREVVSNVGLNLL
                     QFHGDETAAQCESLAGVAGLPWLRALRVAADTQPADLVKSALNYSAASGLLFDTHVEG
                     YGGGGKVFDWSLIPAELARRAVLSGGLNAQNVSDAIHRVRPYAVDVSSGIEV"
BASE COUNT          603 a         1055 c         1002 g          496 t
ORIGIN      
        1 gccggtgttc ccgtggctga aggctcgctg gcgcaggacg ccgcggcggc ttccgcgcga
       61 gcggccgagc cggaagatgg cgtttcgacg cgcgaacaga ttcctgtgcg ggaagcgtcg
      121 gatgcgagcg acgaagcggc agcgtatgga gtagcactgt ctgcggctgg ccgtgagggc
      181 gcggcgcgag aagtagcgga gcgtgaagca gcggcgcgag aagtagcgga acgcgtggcc
      241 gcggagcgcg aagcggaaat tcgcgcagcg gctgcacggg aagcagcgca gcgtgaagct
      301 gcagcgggag aagctgcaaa gcgcttagcg gcagaacatg cagcaaccga gcatacggtg
      361 ggagaaagta cagcagcgca acaagctgct gcggagcgtg ccgaagctga acgagtcgcg
      421 gcggaccagg cggcgcaaga gcgcgcggca ggcggagagc aagctgcagc cagagagcaa
      481 gccgcagcag gacagcaagc cgcagcaaaa gagcaagccg ccgaagaaca cgaagcctcg
      541 accgacgaat cgcacacagc cttccccaca caagaaccca acgcatcccc gcccgtgtgg
      601 ccggaaagtc acgcggcacc cgcgccgctt gaacccgcgc aaccgcgcgc agacatcgaa
      661 ccggctgccc aaagcggctc cacgtccgaa ccgcttccct cagctcatca cgagaacctc
      721 gacggagcgg ccacggctgc gagccttgca gccgccgcgg aacttggtgc tgaagcgttg
      781 ccactgacgt cgcttgaacc ggtcagcgag actttccagc aagaggcgcc tccacaccgt
      841 ctgcaatggg acgatgaacc cgcatcgagc gcgccggccg cgccgctggc agagtcggcg
      901 aatgagtcgc agacccgttt cgacactccc gcaccggtca tcgacttcac gccgcatgag
      961 gccgcgccgc gcacgacgtc gccgttcgat cagccgtcca tcgacacgtc ggcgcatccg
     1021 gcgcccgaca cgtctgtgcc ggcagtgccc gcggtccaac agcacacggc ccacgtcgac
     1081 cccgcctata agccgtccca tcagccacaa ccgcaagcgc aacaacagtc acatcagcaa
     1141 gccgaaaccg caacacccga tggcgcaccg actctcgaac cggcccccgt cgagcaaact
     1201 caacctgcgc gttccattcc atccgagttc ccgcgcgacg ccgtcgatgc cttcagcagt
     1261 ctgaacatgc ccttgccgcc gcgcgtcgaa tcgaccgacg cagcggagag cgcacctgaa
     1321 tcgctttcga cacagcccgt agcctccccg gaagccaccg cacagcaagc cgtggcgcct
     1381 cacgatccgg acgacacccc gcacgtgtcc gacgaattct ccgccggcac cgcaggccac
     1441 gcagccgtgg cgggccttgg cgcggtcggc ttcggcgcgt tgaagctcga tttcgacctc
     1501 gagttgccgc cgagtccggc tcagccgttg cccgccttta cgcgagccga tctcagccgc
     1561 atcgcccgca acaagctcga tctggccgcg gaatatatcg acctcggcga tctgtccggc
     1621 gcgcgcctgc tcatcaacga agtgatcgaa gcgaacgatc cggccacgcg caccgaggcc
     1681 cgcgcgctgc tctcgacgct cgccccgttg tcgtgaagcg tattgcgcta ggcgttcagt
     1741 acgacggctc ggcattctgc ggctggcagt cgcagccgca tggcaagacc gtgcaggacg
     1801 agctcgaacg ggcgttgcgc gagttcgcgc aagtgcctgt gcagacggtg gtggctggcc
     1861 gcacggatac tggcgtgcac ggtctcggcc aggtcgtgca cttcgacacc gaactcgatc
     1921 gcacggattt ttcgtgggtt cgcggcacca acgcctttct gccgaagacg atcgccgtgc
     1981 agtgggccaa accgatgccc gacgcgttcc acgcgcgatt ttcggcgttc gagcggactt
     2041 attactatgt cctgtacgtg catccgttcc gctcgcccat gcaggccacg cgggccggct
     2101 gggtgcacac gccgctcgac gtcgacgcca tgcaggccgc cgccgcgcac ctgatcggcg
     2161 agcacgattt ttcggcgttc cgttcgtcgc aatgccaggc gaaaacgccg gtcaaacatc
     2221 tgtatcagat cgacatgcgg caacagggcg acttcgttca tttccgcttt cgggcgaacg
     2281 cgttcctgca ccatatggtg cgcaacctga tgggctgtct cgtcgatatc ggccggggac
     2341 gccatccggc tgaatggatg gccgaagtgc tagccagccg cgatcgcgac tgtgccgcgc
     2401 cgaccttcat gcccgacggc ctgtacctgg cgcgggtggg ctatcctgag caattcgccg
     2461 tgcccgcgcc gcagacaggc agcgtgccgt ggaataccgt atggaccgag caggcgcaaa
     2521 catgaaatca accgaaaatc tggcggccga aactgacgcc gccatgccgc aacaagcggc
     2581 accgcgtcgc acgcgtatca agttatgcgg cctgtcgaag ccggaagacg ttgcccaggc
     2641 gatcgacctc ggcgccgacg cgatcggact tgtgttctat ccgccgagcc cgcgttcggt
     2701 cagcgtcgcg caggcggtcg agttggtgca tgacgtgccg ccgttcgtgt cggtagtcgg
     2761 cctgttcgtg aatgcaacgc acgactggat tcgcgaagtc gtcagtaatg tcgggctcaa
     2821 cctgttgcag ttccacggcg acgaaaccgc ggcgcagtgc gaatccctcg ccggcgttgc
     2881 gggtttgcct tggttgcgcg cgttgcgagt cgcggcggat actcaaccgg cagatttggt
     2941 aaaatcagcg cttaactatt cagccgccag cggccttctg ttcgacaccc atgtcgaagg
     3001 ctatggcggc ggcgggaagg ttttcgattg gtcacttatt ccagcagagc tcgcgcgtcg
     3061 ggccgttttg agtggtgggt tgaacgcgca aaacgtcagt gatgcgatcc atcgcgtgcg
     3121 cccgtacgcg gtcgatgtct cgagcggcat cgaagt
//
LOCUS       DATASH010000053         1625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_203865,
            whole genome shotgun sequence.
ACCESSION   DATASH010000053 DATASH010000000
VERSION     DATASH010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203865"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1538)
                     /locus_tag="VHV99_01695"
     CDS             complement(<1..1538)
                     /locus_tag="VHV99_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HEX3634202.1"
                     /translation="MDAIRNKPRSTPTASDSSAPAGFVRVRGAREHNLKNIDVQIPRD
                     ALVVFTGVSGSGKSSLAFGTLYAEAQRRYFESVAPYARRLIEHVGVPEVDSIEGLPPA
                     VALQQQRGTPSARSSVGSVTTLSSLVRMLYSRTGDYPPKQPMLFAEDFSPNTVQGACP
                     ACHGLGRVYEVTEKSMVPDDSLTIRERAIAAWPPAWHGQNLRDILVTMGYDVDTPWRD
                     LPKKDRDWILLTDEQPTVPVYAGLTPKETRAALKRKEEPSYMGTFTGARRYVLHTFAN
                     TQSALMKKRVSRYMVGSICPTCHGKRLKKEALSVKFAGLDIGEFAQLPLARLAALLEP
                     IARGEWPDAATGADCKGSVLSRHAMRDAVDKRVAGGGSAHKASPDVRRTPNLSEEKRV
                     AAQRISAELLERLTTLIDLGLGYLALERSTPSLSSGELQRLRLATQLSSQLFGVVYVL
                     DEPSAGLHPADGEALFAALQGLKAAGNSLFVVEHDLQMMRRADWMVDVGPAAGEAGGH
                     VVYSG"
BASE COUNT          265 a          521 c          554 g          285 t
ORIGIN      
        1 ccgctataga ccacatgacc gcctgcttcg ccggctgcgg ggccgacgtc gaccatccag
       61 tcggcgcgtc gcatcatctg cagatcgtgt tcgacgacga atagcgaatt gcccgccgcc
      121 ttcagtcctt gcagcgcggc gaagagcgct tcaccatcgg cggggtgcaa gccggccgac
      181 ggttcgtcga gcacatacac gacgccgaac aactgcgacg acaactgcgt ggccagcctc
      241 aagcgctgca gttcacccga tgacagcgac ggcgtgctgc gctccagtgc gaggtagccg
      301 aggccgagat cgatcagcgt cgtcaggcgc tcaagcagtt cggctgaaat gcgctgcgcg
      361 gcgacgcgtt tctcctcgga gagattcggc gtgcgccgta catcgggcga ggctttgtgc
      421 gccgagccgc ccccggcgac ccgtttgtcg acggcgtcgc gcatcgcatg cctgctgagc
      481 acgctgcctt tgcaatcggc gcctgtcgct gcatcgggcc attcgccccg cgcgatcggc
      541 tcgagcaggg ccgcgagccg ggcgagcggc aattgcgcga attcgccgat gtcgagaccg
      601 gcgaacttca ccgagagcgc ttctttcttg agccgtttgc cgtggcaagt cggacagatg
      661 ctgccgacca tgtaccgcga tacgcgcttt ttcatcagcg cgctttgggt gttcgcgaac
      721 gtgtgcagca cgtagcggcg cgcgcccgtg aaggtgccca tatagctggg ctcttccttg
      781 cgcttgagcg cggcgcgcgt ttccttcggc gtcaagccgg cgtagacggg cacggtcggc
      841 tgttcgtcgg tgaggagaat ccagtcgcga tctttcttcg gcaagtcgcg ccacggcgta
      901 tcgacgtcgt agcccatcgt caccaggatg tcgcgcagat tctgtccgtg ccacgcgggc
      961 ggccatgcgg cgatcgcgcg ctcgcggatg gtcagggagt cgtccggcac catcgatttc
     1021 tcggtgactt cgtacacgcg gccaagcccg tgacaagcgg ggcaggcgcc ttgcaccgtg
     1081 ttcggcgaaa agtcttcggc gaacaacatc ggctgtttcg gcgggtagtc gccggtgcgc
     1141 gagtacagca tgcgcacgag actcgatagc gtcgtcacac tgccaaccga agagcgtgcg
     1201 ctcggcgtgc cgcgctgctg ctgcaatgcg actgcgggcg gcagtccttc gatcgagtcg
     1261 acttcgggca cgccgacatg ctcgatcagg cgccgcgcat acggtgcgac cgactcgaag
     1321 tagcgccgct gcgcctcggc gtagagcgta ccgaacgcca gcgacgattt gccggaaccg
     1381 gacacccccg tgaacacgac cagcgcatcg cgcgggatct gcacgtcgat gtttttgagg
     1441 ttgtgctccc gggcgccgcg cacgcggacg aagccggccg gggcagacga atcggatgcg
     1501 gtcggcgtcg agcggggctt gttgcggatg gcgtccatgt cgatggatag tgggtaggcg
     1561 cggcgcccgg ccagccgggc gaacgcatgt ggggccgcac ggaacatgcc cggcgaagtg
     1621 gagat
//
LOCUS       DATASH010000054         2163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_599727,
            whole genome shotgun sequence.
ACCESSION   DATASH010000054 DATASH010000000
VERSION     DATASH010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599727"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1008
                     /locus_tag="VHV99_01700"
     CDS             <1..1008
                     /locus_tag="VHV99_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007740449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX3634203.1"
                     /translation="DIWGKQRAQVAASLGVHNARLAETSAAELEISTDVAQLYYGIQT
                     TYQLIDLLEQSQQVAAFALETHTARAARGLEPQTRVEEARAQQLAVQRQIVAAQGQLK
                     QFRESLRALTGAGQEGLKEIEPVPLPSAQASLPATLSYELLARRPDLQAMRWYVESSF
                     DQIDAAKAAFYPSFDIKAFFGFDSLHLAELFTHASQQINLIPGLTLPIFDGGRLNANL
                     GGARTASNLLIEQYNQAVLDAVRDVASTGSRLQDLDAETQLQARKVQAVAFARDSAEA
                     QYQRGLASRVTAMEARQPLIAEQVALVNLDGQRLSQEIALAKALGGGYRADSPVELKP
                     R"
     gene            1258..>2163
                     /locus_tag="VHV99_01705"
     CDS             1258..>2163
                     /locus_tag="VHV99_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX3634204.1"
                     /translation="MQDDVQDPTPTAHAYPLLIKQLLHTPFVQAPEQEIVYRGQLRMT
                     YATLHERIARLANGLSRLGARHGSTVAVMDWDSHRYLESYFAVPMMGAVLQTVNVRLS
                     QAEIAYTINHAGAEILLVHTDFLPVLEAIKDQLETVHTFVWIDEPGSDAGAHTIPFAT
                     EYEAMLAASEPSYAFPDFDENTRATTFYTTGTTGLPKGVYFSHRQLVLHTLTVMAALS
                     SPMSGQRFHRGDVYMPLTPMFHVHAWGMPYIATVLGVKQVYPGRYVPERLVQLVRDEG
                     VTFSHCVSTILHMLLGCAEAKSVDFS"
BASE COUNT          385 a          719 c          692 g          367 t
ORIGIN      
        1 gatatctggg gcaagcaacg cgcgcaggtg gcggcgtcgc tgggcgtgca caacgcgcgc
       61 ctcgccgaaa cgtcggcggc cgaactcgaa atctcgaccg acgtcgcaca gctttattac
      121 ggtatccaga ccacgtatca gctgatcgat ctgctggagc agtcccaaca ggtcgcggcg
      181 ttcgcgctgg aaacgcacac ggcgcgcgcg gcacgcggcc tcgagccgca gacccgggtc
      241 gaagaagcgc gtgcgcagca actggccgtg caacggcaga tcgtcgcggc acaagggcag
      301 ctcaagcagt tccgcgagtc gttgcgggcc ctgaccggtg ccgggcagga gggtctgaag
      361 gagatcgagc cggtgccgct gccgagcgcc caggccagct tgccagccac cttgtcgtac
      421 gaattgctgg cgcggcgtcc agacctgcag gcgatgcgct ggtatgtcga atcgtcattc
      481 gatcagatcg acgccgcgaa ggccgcgttc tatcccagct tcgacatcaa ggccttcttc
      541 ggcttcgact cgctgcatct ggccgaactg ttcacgcacg cgagccagca gatcaacctg
      601 attcccggcc tgaccctgcc gatcttcgac ggcgggcgcc tcaatgcgaa tctgggtggc
      661 gcgcgcacgg cgagcaatct gctgatcgag cagtacaacc aggcggtgct cgatgccgtg
      721 cgcgacgtcg cgtcgaccgg cagccggctg caggacctgg atgccgaaac gcaactgcag
      781 gcgcgcaagg tgcaggcggt ggcgttcgcg cgtgacagcg ccgaggcgca gtaccagcgc
      841 ggcctcgcga gccgcgtcac ggcaatggag gcgcgccagc cgctgattgc cgagcaggtt
      901 gcgctcgtca acctcgacgg gcagcggctc agtcaggaaa ttgcgctggc caaggcgctg
      961 ggcggcggct atcgcgccga ttcgcccgtc gaactcaaac cgcgctgagc gttcgcccag
     1021 cggcacaaac cgcgcggagc gcccgcgcga cggcacaagt cgcccgtcag acaggcgctc
     1081 cgggattgcg cgggtttcgc gctccctttg atctcaccca tgccgctccc cctttcaggg
     1141 gatggtccat cacccttatc gcaccgatca tggcatcact cgggatgcac tgaacgcgcc
     1201 acgcgacgac tcacctgtgc cagcccgcga tccagtttcg gactcaagga gatgtagatg
     1261 caggacgacg tgcaagaccc gacgcccacg gcccatgcgt atccgctgct gatcaagcag
     1321 ttgctgcata cgccgttcgt tcaggccccg gagcaggaaa tcgtctaccg cggtcagctt
     1381 cgtatgacct atgccacgct gcacgaacgc attgcgcggc tcgccaacgg cctgagccgg
     1441 ctcggcgcgc ggcatggcag cacggtcgcc gtgatggact gggacagtca ccgctatctc
     1501 gagtcttact tcgcggtgcc gatgatgggc gccgtgctgc agacggtcaa tgtgcggctc
     1561 tcgcaggcgg agatcgctta tacgatcaac cacgccggtg cggaaatact gctcgttcac
     1621 accgattttc tgccggtgct cgaggccatc aaggatcagc tggaaacggt gcacaccttc
     1681 gtctggatcg acgagccggg cagcgacgcc ggtgcgcaca ccattccctt cgcgaccgag
     1741 tacgaggcga tgctcgccgc cagcgagccc agctacgcat tcccggattt cgacgagaac
     1801 acccgtgcca cgacgttcta cacgacgggc accaccggct tgcccaaggg cgtctatttt
     1861 tcgcaccggc aactcgtgct gcataccctc acggtgatgg cggcgctgtc cagtccgatg
     1921 tcggggcagc gctttcaccg cggcgacgtg tacatgccgc tcacgccgat gttccacgtg
     1981 cacgcgtggg gcatgcccta catcgccacg gtgcttggcg tgaagcaggt ttatccgggc
     2041 cgctatgtgc cggaacggct ggtgcagctc gtgcgcgacg aaggcgtcac gttctcgcat
     2101 tgtgtgagca cgatcctgca catgctgctg ggctgcgccg aggcgaagtc ggtcgatttc
     2161 agc
//
LOCUS       DATASH010000055         3668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_203963,
            whole genome shotgun sequence.
ACCESSION   DATASH010000055 DATASH010000000
VERSION     DATASH010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203963"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            443..1216
                     /locus_tag="VHV99_01710"
     CDS             443..1216
                     /locus_tag="VHV99_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3348 domain-containing protein"
                     /protein_id="HEX3634205.1"
                     /translation="MVQAPQRTAFSGPAFIRILARLTEVDVPESSQSLSDRLSQWLGW
                     TDAIALSSALNNAPPAVAAGARSFGDAEESECARVRAALAKAIVGDSVLAAAKRRGPA
                     AQALPHRAPAEAPVDYAVFRQRYLAMQQAMETGVGNLRGRLRAMLAAKTPAMARLAVV
                     DAVMERALSERERSLLAAVPGLLAGHFERLRKAEQQALADAEASADAASAAPATAVAP
                     TPPGAWLDAFRKDMQSVLLAELDVRLQPVEGLLAALRTR"
     gene            1227..3581
                     /locus_tag="VHV99_01715"
     CDS             1227..3581
                     /locus_tag="VHV99_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF802 domain-containing protein"
                     /protein_id="HEX3634206.1"
                     /translation="MSRYLNLVVFLVGLAAVCWIGVGYVGSNALALAVTMLICVCYLA
                     GALELQRYDQATSTLARAVAGLSARPPSLGAWLDPLPPSLRNAVRLRVEGERVALPGP
                     ALTPYLVGLLVLLGMLGTLLGMVATLRGTGMALESATDLQAIRDSLAAPVKGLGFAFG
                     TSIAGVATSAMLGLLSALCRRERIQVAQTLDVKIATTLRIYSQTHQREESLKLLQRQA
                     EVMPTLVDRLQLMMAAIEQQSVASNERQIASQDTFHGRAEAAYRRLAASVEQSLKESV
                     AESTRAASAALQPVMAATMAGLARETAALHETVTQAVQRQLDGLSTGFEATTVSVADI
                     WNKALEGHQRASEALTQQLGTSLDRFTETFEQRSAGLLDGVGARLDATAGSVSAAWNE
                     ALSRQEQAGEKLAGHNQQALAAAAATFEQHSAALVRAVGQSHTDLQTELAARDEQRLT
                     AWTETLGAMAASLSKEWEQMGSHSASRQQEICETLAQTARDISSQTQAHASSTIAEIE
                     RLVQVAAQAPKAAADLQAELAARDQQRLAAWTETLSSMAATLSNEWEQAGTRTATRQQ
                     EICETLAQTARDISAQTQAHASSTIAEIGQLVQAASEAPKAAAEVVAELRQKLSDSMV
                     RDTAMLAERSRLLATLETLLDAVNHASTEQRSAVDALVATSADLLDRVGTRFTDKVEL
                     ETGKLGAIAAQVTGSAVEVASLGEAFGAAVQLFGESNTTLVAHLQRIEAALDKSLARS
                     DEQLAYYVAQAREVIDLSMMSQKQIVEDLQQLAGKRASAGVEAA"
BASE COUNT          662 a         1187 c         1254 g          565 t
ORIGIN      
        1 acgacgagcg cacggtgccg ggcgggcaac gcgtgccgtg ccggagctgg gcgcgacaga
       61 tgcacgacag atgcacgaca gaaacacgac cgaaaacacg acagaagcgt ggggcatcct
      121 agtcgtgaag ctatgacagc cagatgaaat ggccgcatct caacgaggtc ataccgcgtc
      181 ttgcgggagt ggttgcttag cgttggtacg gtacgtcatc gctgcgcccg atcgcctcga
      241 tcactcaata tcgctccatc aatgcgtggt gcaaaaagca gttcgcgtgg cgcaggaaag
      301 gcaacatggt caccgaaatc caggcatcgc gagtggctcg cccgccacgt caaaatccca
      361 ctctcggccg ccctgctcat taaggggctg catccaccac cctgacgacg tgtaaactgc
      421 tgactttttt gactctggtg gtatggtgca agccccacag cgcacagctt tcagtggccc
      481 ggcgttcatt cgcatacttg ctcgcctgac ggaagtcgac gttcccgaat ccagccagtc
      541 gctgtcggac cggttgagcc aatggctcgg ctggaccgac gcgatcgcgc tgtcctcggc
      601 gctgaacaac gcgccgcctg ccgtcgccgc cggcgcgcgc agcttcggcg acgctgaaga
      661 gagcgagtgc gcgcgcgtgc gcgctgcgtt ggcgaaggcg atcgtcggcg atagcgtgtt
      721 ggccgccgcg aagcgtcgcg ggccggcggc gcaagcgctc ccgcaccgcg ccccggcgga
      781 ggcgccggtc gattacgcgg tgtttcgtca gcgctacctc gccatgcagc aggcgatgga
      841 gacgggcgtc ggcaacctgc gcggccgcct gcgggccatg ttggccgcca aaacgcccgc
      901 catggcccgc ctcgcggtgg ttgacgcggt gatggagcga gcgctcagcg agcgtgagcg
      961 gagtctgctg gccgccgtgc cgggattgct cgcaggacac ttcgagcgtt tgcgcaaggc
     1021 ggagcaacag gcgctggccg acgccgaagc ctcggcggac gccgcatcgg ccgcacctgc
     1081 cacagccgtg gcgcccaccc cgcccggcgc atggctggac gcgttccgca aggatatgca
     1141 gagcgtgttg ctcgctgaac tggatgttcg tttacaaccg gtcgaaggat tgctcgcagc
     1201 ccttcgcacc cgctaactgg gacactatgt ccagatatct caatctcgtt gtttttctgg
     1261 taggtctggc cgcggtgtgc tggatcggtg tcggctacgt cggctcgaac gcgctggcct
     1321 tggcggtcac gatgttgatc tgcgtctgct atctggcggg cgcgctcgaa ctgcagcgct
     1381 atgaccaggc cacgtccacg ctggcgcgtg cggtcgcggg gctgtccgcg cggccgccaa
     1441 gcctcggcgc atggcttgat ccgttgcctc ccagcctgcg caatgcagtg cgtctgcgcg
     1501 tcgagggtga acgcgtggcc ttgccgggcc cggcgctgac gccgtacctg gtcggcttgc
     1561 tcgtgttgct gggcatgctc ggcacgctgc tcggcatggt ggcgaccctg cgcggcaccg
     1621 gcatggcgct ggaaagcgcg acggacctgc aggctatccg cgattcgctc gccgcaccgg
     1681 tcaaggggct gggcttcgca ttcggcacct cgatcgcggg cgtggcgacc tcagcgatgc
     1741 tggggctgct gtccgcgttg tgccgtcgcg aacggatcca ggtggcgcag acactcgacg
     1801 tgaagatcgc gacgaccttg cgcatctatt cgcagaccca tcagcgcgag gaaagcctca
     1861 agctcctgca gcggcaggcc gaggtgatgc ccaccctggt cgatcgtctt caattgatga
     1921 tggccgccat cgagcagcag agcgtcgcct cgaacgagcg gcagatcgcc agccaggaca
     1981 cctttcacgg cagggccgag gccgcttata ggcgcctcgc ggcctccgtg gagcagtcgt
     2041 tgaaggagag cgtcgccgaa agcacccgcg ccgccagcgc ggcgctgcag ccggtcatgg
     2101 cggccacgat ggcgggcctc gcgcgcgaga cggccgcgct gcacgagacc gtcacgcagg
     2161 ccgtgcaacg gcaactggac gggctctcca ctggcttcga agcgaccacc gtctccgtcg
     2221 cggacatctg gaacaaagcc ctcgaggggc accagcgagc gagcgaggcg ctgacccagc
     2281 aactgggcac gtcgctggat cggttcaccg agactttcga gcaacgctca gccggcttgc
     2341 tggatggcgt tggcgcgcgc cttgatgcaa cggcaggcag tgtgtcggca gcatggaacg
     2401 aggcgctgtc gcggcaagaa caggccggcg agaagctggc cggccacaat cagcaagccc
     2461 tggccgcggc cgcggcaacc ttcgagcagc actcggcggc gctggtacgc gcggtgggac
     2521 agtcgcatac agacttgcaa acagaattgg ccgcgcgaga cgaacagcga ctgacggcct
     2581 ggaccgagac gctcggtgcg atggccgcat cgctgagcaa ggaatgggag cagatgggct
     2641 cgcacagcgc gagccggcag caggaaatct gcgagacgtt ggcgcaaacc gcgcgcgaca
     2701 tctcctctca aacgcaggcc catgcgagca gtacgatcgc cgagatcgaa cggctcgtgc
     2761 aagtggcggc gcaagccccg aaggctgcgg cggacttgca ggcggaactg gccgcgcgag
     2821 accagcaacg gctggccgcc tggaccgaga cactcagttc gatggccgcc acgttaagca
     2881 acgaatggga gcaagcgggt acgcgcaccg cgacccggca gcaggaaatc tgcgagacgc
     2941 tggcgcaaac cgcacgcgac atctccgcgc aaacgcaggc acacgcgagc agcacgatcg
     3001 ccgagatcgg ccaactcgtg caagccgcgt cggaagcccc caaggccgcc gcggaagtcg
     3061 tcgccgaact gcgtcagaag ctctccgaca gcatggtccg cgacaccgcg atgctggcgg
     3121 agcgcagccg cctgctggcc acgctggaaa cgctgctcga tgccgtgaac catgcctcca
     3181 ccgagcagcg ctcagcggtc gacgcactcg tcgccacgtc ggcggatctg ctggatcgtg
     3241 tcggcacgcg gttcaccgat aaggtcgagc tcgaaaccgg gaagctgggt gcgattgccg
     3301 cgcaagtcac cggtagcgca gtcgaagtgg cgagcctcgg cgaagcattc ggcgcggccg
     3361 ttcaattgtt cggcgagtcg aacacgacgc tggtggcgca cctgcagcgg atcgaagcgg
     3421 cgctggacaa gtccctcgcg cgcagcgacg aacagctggc ctattacgtg gctcaggcgc
     3481 gagaagtgat cgacctgagc atgatgtcgc agaagcagat cgtcgaggac ctgcaacagc
     3541 tcgcgggcaa gcgggcctcc gcgggagttg aagcggcatg aacgaggaaa tcgacggcgg
     3601 cgtggagacg gtggcgccca tctggcctgt cttcggcgac ctgatgtcgg tgctgctggg
     3661 cgccttcg
//
LOCUS       DATASH010000056         2736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2775353,
            whole genome shotgun sequence.
ACCESSION   DATASH010000056 DATASH010000000
VERSION     DATASH010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2775353"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="VHV99_01720"
     CDS             complement(<1..549)
                     /locus_tag="VHV99_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HEX3634207.1"
                     /translation="MKLKQAVTGIAALACMTTAAHAQSAGSFYVTSGWFHLAPQSSSE
                     PLRVTSVGGSPTNLPQANTGASLSSADTLGFTAGYFITDHIATEFVIGIPPTFDLNGT
                     GNLEQFGKLGDAKQWSPTLLFKYYFNAPTATFRPYLGVGVSRIWFTDEKITNGAFEAS
                     VLHGPTSVSTDSSWEPVFNAGFT"
     gene            737..1444
                     /locus_tag="VHV99_01725"
     CDS             737..1444
                     /locus_tag="VHV99_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006052250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX3634208.1"
                     /translation="MARLARLYVPDQPQHVILRGLDQQPAFVDDQDYELFIDCLKAAS
                     RDHHLSIHAYALMPGSVQLLVTPTEESSLPKAMQAVGRRYVAHFNRRYARRGTLWEGR
                     YRATVIEGERYFLLASRVVEMSPVRAGLVSAPEDYRWSSYRHHIGLTLDSLITDHPLY
                     WSLGNTPFERQRAYRELCEQPLDEREASQLQQATLKGWVLGSDTYREWAARAANRRVS
                     PLPRGRPRKVRETPQTQ"
     gene            1742..>2736
                     /locus_tag="VHV99_01730"
     CDS             1742..>2736
                     /locus_tag="VHV99_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HEX3634209.1"
                     /translation="MNDPQQPAHPVPAAQGLYDPQNEHDACGVGFVAHIKGKKSHEII
                     EQGLKILENLDHRGAVGADPLMGDGAGILIQIPDGFYREEMAKQGVVLPPHGEYGVGM
                     VFLPKEHASRLACEQELERTVKAEGQVVLGWRDVPVDHTMPISPTVKASEPLIRQIFI
                     GRGKDIMVTDALERKLYVIRKTASHRIQALKLKHGKEYFVPSCSARTVVYKGLLLAGQ
                     VGVYYRDLQDERTVSALALVHQRFSTNTFPAWELAHPYRMIAHNGEINTVKGNVNWLN
                     ARTGAIASHVLGDDLPKLWPLIYPGQSDTASFDNCLELLVMAGYPLVHAMMMMIP"
BASE COUNT          538 a          852 c          815 g          531 t
ORIGIN      
        1 ggtgaaaccc gcattaaaca ccggctccca cgaactatcc gtggagacgc tggtcggacc
       61 atgcagaaca ctcgcctcga acgcgccatt ggtgatcttt tcgtcggtga accagatgcg
      121 gctcacacca acgccgagat atggacggaa cgtagcggtc ggggcgttga agtagtactt
      181 gaacagcaga gtcgggctcc actgtttcgc gtcaccgagc ttgccgaatt gttcgaggtt
      241 gcccgtgccg ttcaggtcga acgtaggcgg gatgccgatc acgaactcgg tagcgatgtg
      301 gtctgtgatg aagtaaccgg cagtgaagcc aagcgtgtca gctgaactga gtgatgcacc
      361 ggtgttggct tgggggaggt tggtcgggct gccccctacg cttgtcactc tcagcggctc
      421 gctgctcgat tgaggcgcaa gatggaacca gcctgacgtg acgtaaaagc ttccggccga
      481 ttgcgcgtgc gctgctgttg tcatacaggc tagcgccgcg atccccgtta cggcctgttt
      541 taatttcata tgtgctcctc caaaaaaggc ccgctcatta tgactacaac gtttgaaaca
      601 aaccatacgc ggcggttaga gctttctccc taggctccgc gcggtttctg gcttagccgt
      661 gctgcgccga atcccgggcg tgcgcggttc ttcggacctt cgggtatgta ccaacgcgac
      721 tattggatac tccagcatgg cacggcttgc acgtctttat gtccctgacc agccgcaaca
      781 cgtcattctc cgcggactcg atcagcaacc cgcattcgtc gacgaccagg actacgagct
      841 tttcatcgac tgtttgaaag cggcttcacg cgaccatcat ctgtccatcc acgcgtatgc
      901 gttgatgccg ggctcggtgc aactgctcgt cacgccgacc gaggagtcga gcctgccgaa
      961 ggcgatgcag gctgtagggc gccgctacgt cgcgcatttc aaccggcgtt acgcgcgccg
     1021 cggcacgctg tgggagggcc gctatcgcgc caccgtgatc gaaggcgagc gctatttcct
     1081 gctggcaagc cgcgttgtcg agatgtcgcc ggtgcgcgcg gggctggtga gcgcgccgga
     1141 ggactatcgc tggtcgagct accgccatca catcggcctg acgctcgaca gcctgattac
     1201 cgaccacccg ttgtactggt cgctcggcaa cacgccgttc gaacggcagc gcgcttaccg
     1261 cgaactgtgc gagcagccgc tcgacgaacg tgaagccagt cagcttcagc aagccaccct
     1321 gaaaggctgg gttctgggca gcgataccta ccgcgaatgg gctgcgcggg ccgccaaccg
     1381 gcgcgtatca ccgctgccac gcgggcggcc acgcaaggtg cgcgaaacgc cccagacgca
     1441 ataaagcgtt tcaaatcaag cggcttcgcc ccgaacggca ttttcgaatg ccgttttctt
     1501 ttccactttt acgatatggc accaataaaa aacgaccgca atgcaccaac ttgataattg
     1561 gggatcgacc atcacgtttt atttgctatt ccattgattc gtcgcgtata ttccgaattc
     1621 cggcgctttt tggtatgcgc agcatcacgc gtactgagtt cgccgacgtg gttgcggtgc
     1681 gaactgcggc aatagaaaaa cggtttaacg gcctgtccgg cccctcacag acggtgtccc
     1741 catgaacgac ccccagcaac ccgctcaccc ggttcccgcc gcgcaaggtc tgtacgaccc
     1801 gcaaaacgaa catgacgcct gtggcgtcgg cttcgttgcc catatcaagg gcaagaaaag
     1861 ccacgagatc atcgagcaag gcctgaagat tctcgagaac ctcgaccacc ggggcgccgt
     1921 cggcgccgat ccgctgatgg gcgacggtgc cggcatcctg atccagattc cggacggttt
     1981 ctatcgtgag gaaatggcca agcagggcgt cgtgttgccg ccgcacggcg aatacggcgt
     2041 cggcatggtc ttcctgccga aggaacacgc ctcgcgtctc gcctgcgaac aggagctgga
     2101 gcgcacggtg aaggccgaag gccaggtcgt gctgggctgg cgcgacgtgc cggtcgacca
     2161 caccatgccg atttcgccca ctgtcaaggc gagcgagccg ctgatccggc agatcttcat
     2221 cggccgcggc aaggacatca tggtgaccga cgcgctcgag cggaagctgt acgtgatccg
     2281 caagaccgcg agccaccgca tccaggcgct caagctcaag cacggcaagg aatacttcgt
     2341 gccgtcgtgc tcggcgcgca cggtcgtcta caagggcctg ttgctggccg gtcaggtcgg
     2401 cgtgtattac cgcgacctgc aggacgagcg caccgtgtcg gcgctggcgc tggtgcacca
     2461 acgcttctcc accaacacgt tcccggcgtg ggaactggct cacccgtacc gcatgatcgc
     2521 ccacaacggc gaaatcaaca cggtgaaggg caacgtcaac tggctgaacg cccgtaccgg
     2581 cgcgattgcg tcgcacgtgc tcggcgacga tttgccgaag ctgtggccgc tgatctatcc
     2641 gggccaatcg gacaccgcct cgttcgacaa ctgtctcgaa ctgctggtga tggccggcta
     2701 tccgctcgtc cacgccatga tgatgatgat cccgga
//
LOCUS       DATASH010000057         1700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4556796,
            whole genome shotgun sequence.
ACCESSION   DATASH010000057 DATASH010000000
VERSION     DATASH010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4556796"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VHV99_01735"
     CDS             complement(<1..456)
                     /locus_tag="VHV99_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEX3634210.1"
                     /translation="MNIEQARFNMIEQQIRPWEVLDQDVLNLLSIVKRENFVPAVYRE
                     LAFVDFEVPLPAGQHMLAPRVEARVLQELAVKKHESVLEIGAGSGYMAALLAHRAQHV
                     LTVDIEPELAELAKNNLIANGVLNAEVATGDASRGWADAAPYDVICVSGG"
     gene            826..>1700
                     /locus_tag="VHV99_01740"
     CDS             826..>1700
                     /locus_tag="VHV99_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX3634211.1"
                     /translation="MVQPLRPAEGLSIAQVAQRAGVSVATVSRVLNGHENVRPATRDK
                     VLAVVDASGYRVNELARNLRTAESRLLLTMVPDVGNPFYAEIVRGIDSVARQHGYFML
                     LCDTGADPGRERSYFDLLRRRRADGAICLDPATIQQALGEASSALPWVACCEFDPAMG
                     VPYVGIDNYRAAGDAVRHLLARGHRRIGLINSDVDYLYARQRQQGYLDALTEAGITPD
                     ERWRMNLNSLDYEAGASAAALLTQQPDAPSAIFAVSDTLAIGVIHGLRSVGKRVPEDV
                     AVVGFDDISLAAQIDP"
BASE COUNT          262 a          544 c          571 g          323 t
ORIGIN      
        1 cccgcccgat acgcagatca cgtcgtacgg tgcggcgtcg gcccagcccc gcgaggcgtc
       61 gcccgtggcg acttcggcgt tcagcacgcc gttggcgatc aggttgtttt tggccagttc
      121 cgccagttcc ggctcgatgt cgaccgtcag cacgtgctgc gcgcggtgcg cgagcaacgc
      181 ggccatgtaa cccgagcccg cgccgatttc gagcacgctt tcgtgcttct tcaccgccag
      241 ttcctgcagc acgcgcgctt cgacgcgcgg cgccagcatg tgctggccag ctggcaacgg
      301 cacttcgaag tcgacgaagg ccagctcgcg atacacggcg gggacgaagt tttcacgctt
      361 gacgatcgag agcagattca gcacgtcctg gtcgagcact tcccaggggc ggatctgctg
      421 ttcgatcatg ttgaaacgcg cttgctcgat gttcatggtg ggttcggcag tgaggtttgg
      481 cgagtttgcg attgggtttg cgtttttttg ccactggccg gcgggttcgg gatgcgactc
      541 gggggctatc gccccgggca caactcagtt cgcgggtcac ggcagggtaa ctgcgggatt
      601 gtaccaaatg acggcgcgaa cccaggccgt cgagggcagt gcgcagcctg gagcggccct
      661 tcccgcgctg cttcgcctcc gtgttaatcc ctagcgatcc gtccttgcga ttcccgtcgc
      721 gatcgcccaa aaatgtaatc gattacattc tcaaggcgca ttcacttgtc caagtcgttg
      781 tccgaaccgg ccctcacaac gtcgtcgaag gcgggtgcgc agcctgtggt tcagccgttg
      841 cgcccagccg aggggctttc gatcgctcag gtggcgcagc gggcgggcgt ctcggtggcg
      901 accgtatcgc gtgtgctgaa cggtcacgag aacgtgcgcc ctgccacccg cgacaaggtc
      961 ctggcggtgg tcgacgccag cggttatcgc gtcaacgaac tggcgcgcaa cctgcgcacc
     1021 gccgaaagcc gcctgctgct gacgatggtg cccgacgtcg gcaacccctt ctacgcggag
     1081 atcgtgcgcg gcatcgacag cgtcgcgcgt cagcacggct atttcatgct gctctgcgac
     1141 acgggcgccg atccgggccg tgagcgcagc tacttcgatc tgctgcgccg ccgccgcgcg
     1201 gacggcgcga tctgtctcga tccggccacg atccagcagg cgctcggcga ggcatcgagc
     1261 gcgctgccgt gggtcgcgtg ctgcgagttc gacccggcga tgggggtgcc gtacgtcggc
     1321 atcgacaact atcgggcggc gggcgacgcg gtgaggcatt tgctcgcgcg cggccatcgg
     1381 cgcatcgggc tgatcaattc agatgtcgac tacctgtatg cgcggcagcg ccagcaaggt
     1441 tatctggatg cgttgactga agccggcatc acgccggatg agcgttggcg gatgaacctg
     1501 aacagtctcg actacgaagc gggcgcctcg gcggcggcct tgctgacgca gcagcctgac
     1561 gcgccgagcg cgatcttcgc ggtctccgac accctggcga tcggtgtgat ccacggtttg
     1621 cgcagcgtcg gcaagcgcgt gccggaggac gtcgcggtgg tcggcttcga cgatatttcg
     1681 ctcgcggcgc aaatcgatcc
//
LOCUS       DATASH010000058         5742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3765827,
            whole genome shotgun sequence.
ACCESSION   DATASH010000058 DATASH010000000
VERSION     DATASH010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5742
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3765827"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /gene="paaC"
                     /locus_tag="VHV99_01745"
     CDS             complement(<1..711)
                     /gene="paaC"
                     /locus_tag="VHV99_01745"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HEX3634212.1"
                     /translation="MNITPQHLQYVLRLADTALILGQRNAEWCGHGPILEEDIALSNM
                     SLDLIGQARLLYTHAASLEQQLTGKSRTEDDYAYFRAEREFANYTLAELPHYGPLAGT
                     AQAEKDYAVTIVRNFLYSTLMAHVWTALTASKDEQLAAIASKSIKEASYHVHHAREWL
                     IRFGDGTDESHRRAQAALDYLLPYTREFFSADAVEEAIAAAGIGPLTSDLEAAWLEDV
                     RATLDEATLNLPEPVKHIT"
     gene            complement(720..1004)
                     /gene="paaB"
                     /locus_tag="VHV99_01750"
     CDS             complement(720..1004)
                     /gene="paaB"
                     /locus_tag="VHV99_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014895631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HEX3634213.1"
                     /translation="MNKEWPIWEVFVRSKQGLDHKHCGSLHAADAPMALRMARDVYTR
                     RQEGVSIWVVPSSAITASAPEDKAELFEPAGDKIYRHPTFYTLPDEVNHM"
     gene            complement(1044..2042)
                     /gene="paaA"
                     /locus_tag="VHV99_01755"
     CDS             complement(1044..2042)
                     /gene="paaA"
                     /locus_tag="VHV99_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HEX3634214.1"
                     /translation="MYTQSLDIPGNVAPLDAAASAPEQAQFDAVMAADGKIEAQDWMP
                     EAYRKTLVRQISQHAHSEIVGMLPEGNWISRAPSLKRKAILLAKVQDEAGHGLYLYSS
                     AETLGVSRDQLIAALHSGKAKYSSIFNYPTPTWADVGVIGWLVDGAAIMNQIPLCRCT
                     YGPYARAMIRICKEESFHQRQGFDALMSMMSGTDAQRELVQQAVNRWWWPVLMMFGPS
                     DKDSIHSNQSSKWGIKRISNDDLRQKFVDATVDQAKVLGVTLPDPDLKWNEARGHHDY
                     GDIDWEEFWRVVNGDGPCNRERLVTRVKAHNEGAWVREAALAHAEKQRQRAQQQAA"
     gene            2259..3875
                     /locus_tag="VHV99_01760"
     CDS             2259..3875
                     /locus_tag="VHV99_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX3634215.1"
                     /translation="MLRLSEIKLPLDHPESVLEAAVRARLAELGVGDDGLIRYTVFRR
                     AHDARKRADIKLTYIVDVEVKDEAAALKRLADVPHCGVTPDMTYRFVAKAPAQMTSPR
                     PVVIGMGPCGLFAGLILAQMGFRPIILERGKAVRERTKDTFGLWRKSVLNPESNVQFG
                     EGGAGTFSDGKLYSQIKDPKHYGRKVLDEFVRAGAPEDILYLSRPHIGTFRLVSMVEK
                     MRATIHELGGEVRFEARVDDIDIDQGKVRGLKLSTGEDLRCDHVVLAVGHSARDTFQM
                     LSDRGVYIEAKPFSLGFRIEHPQGLIDRSRFGKFAGHKQLGAADYKVVHHCSNGRAVY
                     SFCMCPGGTVVAATSEPGRVVTNGMSQYSRAERNANAGIVVGITPEDYPGGPLAGIAF
                     QRKWEERAFELGGGNYMAPGQLVGDFIAGRPSTSLGSVVPSYKPGVHPTDLSTALPDY
                     VIEAIREALPEMDKKIAGFAMHDAVLTGVETRTSSPIRVRRRDDYQSMNVEGLYPAGE
                     GAGYAGGIYSAAIDGIEVAEALALKMVGAC"
     gene            4194..5462
                     /locus_tag="VHV99_01765"
     CDS             4194..5462
                     /locus_tag="VHV99_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX3634216.1"
                     /translation="MTIRTLAAACSAALLCQFAVSSTVFAADAPTNWVTAWATALQPI
                     PQRSDLPPLYRAPEVAGRTVRQIIYPTLSGKSARVHLSNEYGKTALVIEELRIARSAG
                     GAAALSNGETRVTFGGRRSVSIPAGAELDSDPVPIDIAEGAPYAISAFTGPAQRIVAW
                     HRVANQLNYVSAPGNHTADTSADSFRGRFTQYVWVTGLSVDAPSSAAVAAIGDSITDG
                     MRSSLNQNRRWPDALARRFARSGDHSTAIVNLGISGNRLLRDSLCYGDALVRRFDRDV
                     LERPGVKTAILLIGINDINFAEMPARNGLDCDSPHTSVTAADLIGGYQRVIADARHRG
                     VKVFGATVTPASLPASRETIRLAVNQWIRTSHAFDGVVDFDAALRDPAQPSRLQRTFD
                     SGDHIHPSDAGYAAMAEAIPVAALVSSTRK"
     gene            complement(5513..>5742)
                     /locus_tag="VHV99_01770"
     CDS             complement(5513..>5742)
                     /locus_tag="VHV99_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634217.1"
                     /translation="RIHYRNRTARCWHQSTPQPPGTASFCVAASAAEKRDYEASFSFR
                     QQYFLLSLKTLLPLRPLVSTAALVHKRGGEL"
BASE COUNT          996 a         1694 c         1870 g         1182 t
ORIGIN      
        1 cgtgatgtgt ttgaccggct ccggcaagtt gagtgtggct tcgtcgagcg tggcccggac
       61 gtcttcgagc catgcagctt cgaggtccga cgtcagcggt ccgatgccgg cggcggcgat
      121 ggcttcttcc acggcatccg cgctgaaaaa ctcacgcgtg tacggcagca gatagtcgag
      181 cgcggcttgg gcgcggcgat gcgactcgtc cgtgccgtca ccgaaacgga tgagccattc
      241 ccgcgcgtgg tgcacgtgat agctggcttc cttgatcgat ttcgaggcga tcgcggcgag
      301 ctgttcatct ttggacgcag tgagggcggt ccacacatgc gccatcagcg tcgagtagag
      361 gaaattgcgc acgatcgtga ccgcgtagtc cttctcggct tgcgcggtgc cggcgagcgg
      421 accgtagtgc ggcaactcgg ccagcgtgta gttggcgaac tcgcgttcgg cgcggaaata
      481 cgcgtaatcg tcttccgtgc ggcttttgcc ggtgagctgc tgttccagcg aggctgcgtg
      541 cgtatacagc aggcgcgcct ggccgatcaa atcgagactc atattcgaca gcgcgatgtc
      601 ctcttcgaga attgggccgt ggccgcacca ctcggcattg cgctgaccga ggatcagcgc
      661 ggtatccgcg aggcgcagca cgtactgcag atgttggggc gtgatgttca tggccgcgct
      721 tacatgtggt tgacttcgtc ggggagcgta tagaacgtcg gatggcggta aatcttgtcg
      781 cccgccggtt cgaacagctc cgccttgtct tccggcgccg acgccgtaat cgcggaagac
      841 ggcaccaccc agatgctcac gccttcctgg cggcgcgtgt agacgtcgcg cgccatgcgc
      901 agcgccatcg gcgcgtcggc ggcgtgcagg ctcccgcagt gtttatggtc gagtccctgc
      961 ttgctgcgca cgaagacttc ccaaatcggc cattccttgt tcattgctga tctcctgata
     1021 ctttgtctgg cttgtcgccc aggtcaggcg gcttgctgtt gtgcgcgctg gcgctgcttt
     1081 tcggcgtggg cgagggcggc ttcacgcacc catgcgcctt cgttgtgagc ttttacgcgg
     1141 gtcacgaggc gctcgcggtt gcacgggccg tcgccgttca cgacgcgcca gaattcttcc
     1201 cagtcgatgt cgccgtagtc gtggtgcccg cgggcttcgt tccacttcag gtccgggtcc
     1261 ggcagcgtga cgccgagcac cttggcctgg tcgacggtag cgtcgacgaa tttctgacgc
     1321 agatcgtcat tggagatgcg cttgatgccc catttcgacg actggttgct gtggatcgag
     1381 tctttgtcgc tcgggccgaa catcatcagc accggccacc accagcggtt cacggcttgc
     1441 tggaccaact ctcgttgcgc atccgtgccg ctcatcatcg acatcagcgc gtcgaagcct
     1501 tgacgctggt ggaacgactc ttccttacag atacggatca tggcgcgcgc gtatgggccg
     1561 taagtgcagc ggcacagcgg aatctgattc atgatcgccg cgccgtccac cagccagccg
     1621 atcacgccga cgtccgccca cgtgggtgtt gggtaattga agatgctcga atatttggct
     1681 ttgcccgagt gcagcgccgc gatcaactga tcgcgcgaca cgccgagcgt ttccgccgaa
     1741 ctatataaat agaggccgtg gccggcttcg tcctgcactt tggccagcag gatcgccttg
     1801 cgcttcaggc tgggcgcgcg cgaaatccag ttgccttcgg gcagcatgcc gacgatttca
     1861 gaatgcgcgt gctgcgaaat ctgccgcacc agcgtcttgc ggtaggcctc aggcatccag
     1921 tcctgcgcct cgatcttgcc gtcggcggcc atgaccgcgt cgaattgcgc ctgctcgggc
     1981 gcactcgctg ccgcatcgag cggggcgaca ttgccgggga tatccaggga ttgcgtgtac
     2041 atggggacgc tctcagcgga agttgtctgt gtctgcgcag tataaaccaa ccgaccggtc
     2101 ggttaataaa ttttttgaga cgggtcgtga taaccgatag gggaacgagg tggcggtggc
     2161 ggaggcgttt cgcttgcgcc gtcgccccgg gccgacgctt aacctataaa attgcgaatt
     2221 ggccgcgatt tgcggccttc ctgcagcccc cggtacacat gttacgtcta agcgaaatca
     2281 aactcccgct cgatcacccc gaaagcgttc tcgaagccgc cgtccgcgcg cgcctcgctg
     2341 aactcggcgt gggcgacgac gggctcatcc gatacaccgt gttccgccgc gcgcacgacg
     2401 cgcgcaagcg cgccgacatc aagctcacat acatcgtcga cgtcgaagta aaggatgaag
     2461 cggcagcgct taagcggctc gccgacgtac cgcactgtgg tgtgacgccg gacatgacgt
     2521 accgttttgt cgccaaggcg ccggcgcaga tgacctcccc gcgtccggtc gtgatcggca
     2581 tggggccttg cggcctgttt gccgggctga ttctcgccca aatgggcttc cgtccgatca
     2641 ttcttgagcg cggcaaggct gtgcgcgagc gcaccaagga tactttcggg ctgtggcgca
     2701 agtcggtgct caaccctgaa tcgaacgtgc agttcggcga aggcggcgcg gggaccttct
     2761 cggacggcaa gctctacagc cagatcaagg atccgaagca ctatggccgc aaggtgctcg
     2821 atgaattcgt ccgcgcgggc gcgcctgagg acattcttta tctgagccgg ccccatatcg
     2881 gcacgttccg gctcgtcagc atggtcgaaa agatgcgcgc gaccattcac gaactgggcg
     2941 gcgaggtgcg tttcgaggcg cgagtcgacg atatcgacat cgatcagggc aaggtgcgtg
     3001 ggctcaaact gtcaacgggc gaggatttgc gctgtgatca tgtggtgctg gcggtcggcc
     3061 atagcgcgcg cgataccttc cagatgctga gcgaccgcgg cgtctatatc gaagcgaagc
     3121 cgttttcgct cgggtttcgg atcgaacatc cgcaagggtt gatcgatcgc agccgtttcg
     3181 gcaagttcgc cggccacaag cagttgggcg ccgccgacta taaggtcgtg catcattgca
     3241 gcaacgggcg tgccgtttat agcttctgta tgtgtcccgg cggcacggtg gtcgcggcga
     3301 cgtccgagcc gggccgcgtg gtgaccaacg gcatgagcca gtactcacgg gcggagcgca
     3361 atgcgaatgc cgggatcgtc gtcggcatca cgccggagga ttatccgggc ggcccgctcg
     3421 cgggcatcgc tttccagcgc aagtgggaag agcgggcgtt cgaactgggc ggcggcaact
     3481 atatggcgcc ggggcaactg gtgggcgact tcatcgccgg tcggccgtct acgtcgctgg
     3541 gttcggttgt gccatcctac aagccgggcg tgcatccaac cgatctcagc acggcgctgc
     3601 ccgactacgt gatcgaagcc atccgcgaag cgttgcccga gatggacaag aagatcgccg
     3661 ggtttgccat gcatgacgcc gtgctgacag gcgtcgagac gagaacatcg tcgccgattc
     3721 gcgtgcggcg ccgggatgac tatcaaagta tgaatgtcga gggcttgtat ccggccggtg
     3781 agggagcggg gtatgccggc ggaatttatt cggcagccat cgacgggatc gaagtggccg
     3841 aagcgcttgc gttgaaaatg gttggggctt gttaagcgac tgatttgtaa tacattttcg
     3901 ctcgcgtcga tggttgcggc gcgcataacg tggcttgggg tgggcggtgt gaggtggtgc
     3961 gatcggctgc tttgccgacg tcgccgctac agttcttgcg agcgttctag tgcgcggagc
     4021 cgaatgggtt cggttgtgcg ctgttcactg actatccata tcgctcttgc ggtgtattgg
     4081 acccgcagcc ctgctgccag gcgcggggct cgcacgatcg gatcgctcct ggttagccgc
     4141 ggtggtatga cgatcgggca caatgcacgt ttcgcctcga accgactacc cgaatgacca
     4201 ttcgcaccct ggccgccgca tgtagcgcgg cgctgctttg ccaatttgcc gtttcctcga
     4261 ctgtttttgc cgccgacgcc ccgacgaatt gggtcactgc atgggcgacg gcgttgcaac
     4321 cgatcccgca gcggtcggat ttgccgccgt tatatcgtgc cccggaggta gcagggcgta
     4381 cggttcgcca gatcatttat ccgacgctgt cgggcaaatc cgcacgagtc catctcagca
     4441 atgagtacgg taagacagcg ctcgtgatcg aggaactgcg catcgcgcgc tcggccggcg
     4501 gcgcggcggc gctgagcaat ggcgagacgc gcgtgacgtt tggcggtagg cgttcggtga
     4561 gcataccggc gggtgcggag ttggacagcg acccggttcc tatcgatatt gccgaaggtg
     4621 cgccttatgc gatcagtgca ttcacggggc cggcacagcg gatcgtggcg tggcatcgag
     4681 tcgcgaatca attgaactac gtgtccgcac cgggtaacca cacggcagac acttccgctg
     4741 attcgtttcg gggccggttc acgcaatacg tgtgggtgac gggtttgtcg gtggacgcgc
     4801 cgtcatcggc tgccgtcgcc gcgatcggcg actcgatcac cgacggcatg cgctccagtc
     4861 tgaatcagaa tcggcggtgg cccgacgcac tggcgcgtcg gttcgcccgc agcggcgacc
     4921 actcgacggc gatcgtgaat ctgggcatca gcggcaatcg cttactgcgc gactcacttt
     4981 gctacggtga cgcgctcgtc cggcgtttcg accgggacgt gctggaacgt ccaggcgtca
     5041 agacggcgat tttgctgatc ggtatcaacg acatcaactt tgcggagatg ccggctcgta
     5101 atggccttga ctgcgactcc ccgcatacct cggtgactgc cgccgacctg attggcggct
     5161 accagcgggt gattgcggat gcacggcacc gcggcgtgaa agtgttcggc gcaaccgtga
     5221 cgccggcatc gctgccggcg tcacgggaga cgatccggct tgcggtcaac cagtggattc
     5281 gaaccagcca cgctttcgac ggcgttgtag atttcgacgc cgcgctacgc gacccggcgc
     5341 aaccgagtcg cctgcagcgc acctttgaca gtggcgatca tattcatccc agcgacgccg
     5401 gctatgccgc aatggccgaa gcgattccgg tggctgcctt ggtgagttca acccggaagt
     5461 gagttgccga aaaagatttt cgtcaaaccc ttgtcacgtg gctgatgttc acctatagtt
     5521 cgcctcctcg tttgtgaacg agcgccgcgg tagaaaccag cggccgtagc ggcaacaggg
     5581 ttttaagcga aagcaaaaaa tattgttgac gaaacgaaaa agaggcctca taatctcgtt
     5641 tctctgctgc tgatgcagcg acgcagaacg aagcggtacc gggtggttgt ggtgttgatt
     5701 gatgccagca gcgcgcggtg cggttccggt agtgaatgcg ga
//
LOCUS       DATASH010000059         5437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2183494,
            whole genome shotgun sequence.
ACCESSION   DATASH010000059 DATASH010000000
VERSION     DATASH010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5437
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2183494"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(221..673)
                     /locus_tag="VHV99_01775"
     CDS             complement(221..673)
                     /locus_tag="VHV99_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX3634218.1"
                     /translation="MTQRPALPFTLDEQLCFALYSTSLAMTKAYKPLLDKLGLTYPQY
                     LAMLVLWEADDVTVKDLAARLNLDSATVTPLLKRLEAQGYLERVRGVEDERLVYIRLT
                     KAGTALKRQAREVPAEIFCATQQTPEFLLRLRDDLTRLRATLNDYMDH"
     gene            complement(971..1777)
                     /locus_tag="VHV99_01780"
     CDS             complement(971..1777)
                     /locus_tag="VHV99_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HEX3634219.1"
                     /translation="MQLIEVPAKTGYVWFRQGIWLFRKNPLAFLTLFFTYLLVMTLAA
                     QIPVIGGVLPLVFIPGVAVGFMAACRNTIAGKPVFPTILVDGFHSYGPVVAKRLFVLG
                     VLYVIAMALVLAGSALADGGMLLKVMLGAATMDQDAIANSNIPLAVITAFAFYIPVAM
                     IFWFSPILAAWHDVPPIKAMFFSLVSCWRNRGAFIVFGALWFAVATIVSFGLSALMQA
                     LGAGDFAFAILMPATMIVTTMLYCSFYATYRGCFGVQTPEAPDLPTTPAA"
     gene            complement(1768..2763)
                     /locus_tag="VHV99_01785"
     CDS             complement(1768..2763)
                     /locus_tag="VHV99_01785"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEX3634220.1"
                     /translation="MAVFTAVTEPQLAEWMRHYDLGHVVEFRGIPSGIENSNFFLTTT
                     RGEYVLTIFEKLTAEQLPFYLDLMQHLAAHRVPVPDPMPREDGALFGTLHGKPAAIVT
                     KLEGAPELAPQVEHCVEVGQMLARMHLAGRDYRRYQPNLRSLPWWKETVPTILPFLQG
                     AQRDLLSSELAHQEAFFASADYAALPEGPCHADLFRDNALFAHAEPETGHEVRLGGFF
                     DFYFAGCDKWLFDVAVTVNDWCVDLATGQLDEARTEAMLRAYQTVRPFTVEENRHWGD
                     MLRAGAYRFWVSRLYDFHLPRAAELLKPHDPGHFERILRERLSGVALPGIHTSCN"
     gene            complement(3098..3493)
                     /locus_tag="VHV99_01790"
     CDS             complement(3098..3493)
                     /locus_tag="VHV99_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634221.1"
                     /translation="MKPLLPVAVALALAFGNAAMAAQPVDDTPAPGAPQSANERAGLP
                     DLEKINRPGAEVSSKVEINVPRTPSFHERSANGTEITEYRDKGKPVEINVRSNLGTHY
                     QMSAPLDTSPSVRDNGRPSTRLPSVNLTY"
     gene            3694..5220
                     /locus_tag="VHV99_01795"
     CDS             3694..5220
                     /locus_tag="VHV99_01795"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004529041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HEX3634222.1"
                     /translation="MNNDTNQRAVQTPADSFPTESFDDATEAVTRLSAIYEANTSFLR
                     DAFARYRRNELFERRVRACYPFVRVRTEVNTHIDSRRSYGFVAGPGVFETTVTRPDLF
                     GNYYREQLRLLAKNHHVKIEVGVSDQPIPIHFAFAEGIHLEGDLDRERLFLMRDVFDT
                     PDLALLDDRIVNGTYEPLPGEPHPLALFTAARVDFSLHRLKHYTATSPTHCQNYVLYT
                     NYQFYIDEFVKLGRTMMAHTDDEELRAYRSEYTSFVEPGDVITYNENLGEQEQEGTAP
                     PRLPQMPAYHLKRADGSGITMVNIGVGPSNAKTITDHIAVLRPHAWIMLGHCAGLRNT
                     QRLGDYVLAHGYVREDHVLDDDLPLWVPIPALAEVQVALERAVAQITQLDGVELKRVM
                     RTGTVASVDNRNWELRDHREPVQRLSQSRAVALDMESATIAANGFRFRVPYGTLLCVS
                     DKPLHGELKLPGMADQFYRAQVDQHLQIGVKAMELLRMNGLHRLHSRKLRSFAEVAFQ
                     "
BASE COUNT         1074 a         1578 c         1771 g         1014 t
ORIGIN      
        1 tcacggccac cggtgctggt tgcgcttgct ttgtagagaa tgttcatggt gctgctcctt
       61 tgtctgggtg atgaggatct gcctgtctac cgctgcttgc tcgtccaccg ttccggcggc
      121 gccctatcga agcggcatgt gataaagata gtacacaaat catttgtgtg caaatcaatt
      181 tttgcatcat accgatagcg tgctttttga agcaggctgg ctagtggtcc atgtaatcgt
      241 tgagggtggc gcgcaggcgc gtcagatcgt cgcgcagacg gagcaggaat tcgggtgtct
      301 gctgggttgc gcagaaaatt tccgccggca cttcgcgtgc ctggcgcttc agtgcggtgc
      361 cggctttcgt gaggcggata tagacgagcc gctcatcttc aacgccgcgc acccgttcca
      421 gatagccttg cgcctcgagg cgcttgagta gcggcgtgac cgttgccgag tcgagattca
      481 gacgtgcggc gaggtctttg accgtgacgt cgtcggcttc ccaaagcacc agcattgcaa
      541 gatattgcgg ataggtgagg ccgagcttgt cgagcaacgg cttgtacgct ttcgtcattg
      601 caagcgaggt cgaatagagc gcgaagcaga gttgttcgtc gagcgtgaag ggcaaagcgg
      661 ggcgctgggt catgttgcat ctcgtataaa aagagcgtgc aaatcgattg cgcgcaaacc
      721 attgtgccgc aattcgaggc aggttgacga gtgcggcagg gattcggctc aacgtcggga
      781 gcgcatggcg tgccttgagg cgcaagcgct gagggcaccg ggacgagagt acagggccgc
      841 caaggctggc atgcggaccg ccaggcggga ctagcctagc gaaggacact aagacggaag
      901 cgggcagcgt tccgaatcga agccgcgcgg gcgccaaaaa acgcccgcgc ctgaatagcc
      961 tggccgcggt tcaagccgcc ggcgtggtcg gcaggtccgg cgcttcaggc gtttgcacgc
     1021 cgaagcagcc gcgataagtc gcgtagaacg agcagtacag catggtcgtc acgatcatcg
     1081 tggcgggcat cagaatcgcg aacgcgaagt cgcccgcgcc cagcgcctgc atcaacgcgg
     1141 acaagccgaa cgagacgatc gtcgccaccg cgaaccacaa tgcgccgaac acgatgaacg
     1201 cgccgcgatt gcgccagcaa ctgacgaggc tgaagaacat cgccttgatg ggcggcacgt
     1261 cgtgccacgc ggcaagaatg ggcgagaacc agaaaatcat cgcgaccgga atgtagaacg
     1321 cgaacgcggt gatcaccgcc agcggaatat tgctgttggc aatcgcgtcc tgatccatcg
     1381 tagccgcgcc gagcatgacc ttcagcagca tgccgccgtc agcgagtgcc gagccggcca
     1441 gcaccagcgc catcgcgatg acatacagca cgccgagcac gaacagacgt ttggcgacca
     1501 cggggccgta cgaatgaaag ccgtccacca gaatggtcgg gaacaccggc ttgccagcga
     1561 tggtgttgcg gcacgcagcc atgaagccga ccgcgacacc cggaatgaag acgagcggca
     1621 gcacgccgcc gatgactgga atctgcgccg ccagcgtcat gaccagcaga taggtgaaga
     1681 acagcgttag aaacgcgagc gggttcttgc ggaacagcca gataccttgc cggaaccaga
     1741 catagccggt tttcgcgggg acttcgatca gttgcatgag gtatgaatgc ctggaagtgc
     1801 aacgccggac aggcgctcgc gcagaatgcg ctcgaaatgg ccgggatcgt gcggtttgag
     1861 cagctcggcc gcgcgcggca aatgaaaatc atacaggcgc gagacccaga agcggtaagc
     1921 gcccgcacgc agcatgtcgc cccaatggcg gttttcttcg acggtgaacg ggcgcacagt
     1981 ctggtaggca cgcagcatgg cttcggtgcg cgcttcgtcc agctggccgg tggcgaggtc
     2041 gacgcaccag tcgttgaccg tgaccgcgac gtcgaacagc cacttgtcgc agccggcgaa
     2101 atagaagtcg aagaagccgc ccagccgcac ctcatggccg gtttccggct cggcatgcgc
     2161 gaacaaggcg ttgtcgcgga acaggtcggc gtgacagggg ccttcgggca acgcggcgta
     2221 atccgccgac gcgaagaaag cctcctggtg cgccagttcg gacgacagca agtcgcgctg
     2281 cgcgccttgc agaaacggca ggatggtcgg cacggtttcc ttccaccacg gcaggctgcg
     2341 cagattcggc tgatagcgcc ggtaatcacg ccccgccaga tgcatgcgcg cgagcatttg
     2401 cccgacctcg acgcagtgct cgacttgcgg cgccaactct ggtgcgcctt cgagtttggt
     2461 cacgatggcg gcgggcttgc cgtgcagtgt gccgaacagc gcgccgtctt cgcgcggcat
     2521 gggatccggc accggcacgc ggtgcgcggc cagatgctgc atcaggtcga gatagaacgg
     2581 cagttgctcg gccgtcagct tttcgaaaat cgtgaggacg tattcgccgc gcgtcgtcgt
     2641 cagaaagaag ttgctgttct caataccgga ggggatgccg cgaaactcga cgacgtggcc
     2701 gagatcgtaa tgacgcatcc attctgctag ttggggttcg gtgacagcgg tgaagacagc
     2761 catgcgggaa acgtcggatc agaggttagt cgggctggcg cgatgcggcc agtgcgaggt
     2821 gtggcaaatg cgctgcagac agcgcgatca agctcaatcg ggctgcgagt tgtggccatg
     2881 caactggccg tgaacctggc tacgattctg gccgcgatcc tcgccactcg ccaaagcctt
     2941 aaagcgctgc ggccattgag cgcggcatcg cagtgtttca gaccatgctg caggtggcgc
     3001 taaaaatggc caggcaagct gaaatcccga ggcggcgccc tcgggctgcg taggtcccgg
     3061 ggaagcggtt catgtcgcgc cgccgccccg gcatacatca ataagtcaga ttcactgatg
     3121 gcaaccgcgt gctcgggcgg ccgttgtcgc gcacgctggg cgacgtatcg agcggcgcgc
     3181 tcatctgata gtgcgtgccg agattcgaac gtacgttgat ttcaacgggc ttgcccttgt
     3241 cgcgatattc ggtgatttcg gtgccgttag cgctgcgttc gtggaagctt ggggtgcgcg
     3301 ggacgttgat ttcgaccttc gaactgacct ccgcgcccgg acgattgatc ttctccaggt
     3361 caggcagacc ggcccgttca ttggcggact ggggcgcacc gggggccggc gtgtcgtcga
     3421 caggctgggc agccatcgcg gcgttgccaa aggcgagggc cagtgcaacg gcgacgggaa
     3481 ggagcggctt catggtgatt ctccaatatg gtgccccgat tctagcaaat ccagccttgc
     3541 ccacgggcgc gatcgcctta ttttgcgccg ctttcgcgcg ccgcgcgcgc aatcttgcgg
     3601 ccgctgttgc ggcagttgtg gtagacgccg cggggtcatt tggcgatcac gggttccgtg
     3661 gtaatgtcgt ctcatcaata ggggcgaatg aagatgaaca acgataccaa tcaacgcgcg
     3721 gtgcagactc ccgccgacag cttcccaacc gaatcctttg acgacgccac cgaggccgtc
     3781 acgcgtctgt cggcgatcta cgaagcgaac acttcgtttc tgcgcgatgc cttcgcgcgc
     3841 tatcgccgca acgaactgtt cgaacgccgg gtgcgcgcct gctacccgtt cgtccgggtg
     3901 cgcaccgaag tcaacacgca tatcgactcg cgccgctcgt acggtttcgt cgcgggcccg
     3961 ggcgtgttcg agaccacggt cacgcggccg gatctgttcg gcaactacta tcgcgagcaa
     4021 ttgcgtttgc tggccaagaa ccatcacgtg aagatcgaag tcggtgtgtc tgatcagccg
     4081 attccgattc acttcgcgtt cgctgaaggc atccacctcg agggcgatct ggatcgcgag
     4141 cgcctgttcc tgatgcgcga tgtgttcgat acgccagacc tcgcgctgct cgacgatcgc
     4201 atcgtcaacg gcacgtatga gccgctgccg ggcgagccgc atccgctggc gctgttcacg
     4261 gcggcgcggg tggatttctc gctgcatcgg ctgaagcact acacggcgac ctcgcccacg
     4321 cattgccaga attacgtgct gtacacgaac taccagttct atatcgacga gttcgtcaaa
     4381 ctcggccgca cgatgatggc ccataccgac gacgaagaat tgcgcgcgta ccgcagcgaa
     4441 tacacgtcgt tcgtcgaacc gggcgacgtg atcacgtaca acgagaatct cggcgaacag
     4501 gagcaggaag gcacggcacc gccgcgtctc ccgcagatgc ccgcgtatca cctgaagcgc
     4561 gccgacggca gcggcatcac gatggtcaac atcggtgtcg ggccgtcgaa cgcgaagacc
     4621 atcaccgatc acattgccgt gctgcgtccg catgcgtgga tcatgctcgg ccactgcgcg
     4681 gggctgcgta acacgcagcg cctgggtgac tacgtgctgg cgcacggcta tgtgcgcgaa
     4741 gatcacgtgc tcgacgacga tttgccgctg tgggtgccga tccccgcgct tgccgaagtg
     4801 caggtggcgc ttgagcgcgc ggtcgcgcag atcacgcaac tggatggtgt cgaactgaag
     4861 cgtgtgatgc gcacaggaac ggtggcgagc gtcgataacc gcaactggga gttgcgcgat
     4921 catcgcgagc cggtgcagcg gttgtcgcaa agccgcgcgg ttgcgctcga catggaaagc
     4981 gcgacgatcg ccgccaatgg gtttcggttc cgggtgcctt acggcacctt gttatgcgtg
     5041 tcggacaagc ccttgcatgg cgaattgaaa ctgcccggca tggcggatca gttctatcgc
     5101 gcgcaggtcg atcagcattt gcagatcggc gtgaaggcga tggagttgtt gcggatgaat
     5161 gggctgcatc ggttgcatag ccggaagttg cggagttttg cggaagtggc gtttcagtga
     5221 agggggatgc cgaggtttcg cggaatacgg ttgtgaaaag agcggctgtg tagagaattg
     5281 cccatggagc cgcatgaagt tcacacgccg atcaggtttt ggcatcgcat tgatcttgca
     5341 cgagccctga tgcggcgtgt tttcaatttt gctgtactcc aatttcgctt cgacgcctgt
     5401 ctgatcgcca caccacgatc ggcgccagac agaattg
//
LOCUS       DATASH010000060         5936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5348798,
            whole genome shotgun sequence.
ACCESSION   DATASH010000060 DATASH010000000
VERSION     DATASH010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5936
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5348798"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..720)
                     /locus_tag="VHV99_01800"
     CDS             complement(58..720)
                     /locus_tag="VHV99_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014973250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX3634223.1"
                     /translation="MQKLDPTVITHNLQPIAAGLATTLGTWAAGVAIGMLIGFLIAVL
                     QLFCGRWVRGILRFYIELFRGTPFLVQLFLLYYGGPSFGLTLEPMTAGVLGLGLYGSA
                     YFAEAFRSGFQSVPPGHLEAASCLGLTRWQTVLRIQVPQMLVLIVPALTNLIIVLSKE
                     TAVLSIVTVPELTFVLTGIGSATFAFVETLLVLCLCYLALVELTSRVGMWAETRIARF
                     MA"
     gene            complement(750..1400)
                     /locus_tag="VHV99_01805"
     CDS             complement(750..1400)
                     /locus_tag="VHV99_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX3634224.1"
                     /translation="MFSATVFLQGLPLLLHAALATIGISLTGLVIGFFVAIGVCAARL
                     SPKRSLRMFGGAYVFFFRGVPMLVQLLLVYYLLPFAGINVSPIVAALSAVSLCSASYI
                     AEILRGGFLSIAPGQIEAARMLGLAPFDMLRRILVPQAFRLTLPSLVNEMVLLIKASS
                     LISVVGVAELTRTAQNIAASTYRPLEAYVAAGLIYFVICGTLALVAHAAEYRLQHA"
     gene            complement(1414..2241)
                     /locus_tag="VHV99_01810"
     CDS             complement(1414..2241)
                     /locus_tag="VHV99_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006052358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX3634225.1"
                     /translation="MSILAGWKCRLVMLALCAASVTAHAEDQLAKVKKAGELVVGTEM
                     QFAPFDFLENGQQAGFNKDLFAEVGKEMGVKVRFIDLPWPSVLPGLEAGKFDMVGGPL
                     TVTKARMERYTYTLPVADATDALLKRANDTSVKQSSDIVGKTVGAGKGSAQLDQLKAY
                     VATLPKPPEIREYVDNNQAYADLAAGRIAAVANSMTNIAYVAKQRPETFAVVQPPFGA
                     KVYFAYCLRKDADSKPLADAFNAALVKMHNDGRLAALQKKWFGVAMDAPTTMPAPNY"
     gene            2547..3536
                     /locus_tag="VHV99_01815"
     CDS             2547..3536
                     /locus_tag="VHV99_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HEX3634226.1"
                     /translation="MAVTRIPIIDLAGVRAGDPQAVTRVAHEIREACTTIGFFYIVNH
                     GVPQAAIDAAEQAARRFFAFPVETKRRAAVNQRHRGFNALGDATMYQAKRPDYKEFFS
                     IGLELPEDDPDVLAGQALRGPNNWPDFMPELRPALYGYYEAVAACGADLLRAVALSLG
                     VGEQFFAPRYTKRMQRTQMVYYPPQPPQSDADQFGVAPHTDYGCITLLWQDQVGGLQV
                     REIVNDTWVDAPPVEGSFVVNVGDLLARWTNDRFRSTLHRVINASGRERYSIATFYDP
                     TYGAMVDPRELGTSDADLRYQPVAAGDYILGRINDSMGYRKKAAVAGTQGTTA"
     gene            3533..4966
                     /locus_tag="VHV99_01820"
     CDS             3533..4966
                     /locus_tag="VHV99_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX3634227.1"
                     /translation="MTDHSSANARSSAIHSPAPLSATLAALRAALGDDAVRVGEQIGE
                     RAMTDWTRHDPTRPAALLLPRSTEEVSRALAICHGAHQPVVPQGGMTGLAGGAIARAT
                     DIALSLERLTGVEEIDTASATLTVRAGTTLQTAQEAAAEAGFELALDLGARGSCQIGG
                     NLATNAGGNRVIQSGTARDQVLGLEVVLANGDVLTSLGKMVKNNTGYDLKHWFIGSEG
                     TLGVITRAVLRLHPQRAARHTALVALDGYEAAVSLLRRLSTRFGNDIGAFEIMWPDFY
                     DFGVKLTGTRSPFAASHPLYALIEHASFDAGDDGERFSAALTDALDSAAIRDAVIAQS
                     VADVRALWAIRECTAEFPVRLDAINFDVSLPISGIGAFVDRCRAALDQRWPGNASYFF
                     GHIGDSNLHVTVDGHSIPGVDHHAVYAFVYEMLGPLHGSVSAEHGIGLLKREFLPISR
                     SPAELAAMAAIKHALDPHGILNPGKLF"
     gene            complement(5104..>5936)
                     /locus_tag="VHV99_01825"
     CDS             complement(5104..>5936)
                     /locus_tag="VHV99_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634228.1"
                     /translation="QSQGASPIKSISLKTMEQLTFGACVKKGWISSWQAVTQMPWLFL
                     GVCAVLACTSLLAGPHPAAVTPASGVDAMANLGQRLLPLVWTALQLAVGGTLTIKVHR
                     FVLLGEGVQPLVPLNGKPLGRYALVSLAVVLTMLVFAGLGFVAMRTVRLGVLIYLPML
                     AIYLFVTMRLILLYPAIALGSRLVLRAAWKDSRGHVWSMIAVGFVTYLPVFVLSAIFL
                     FIAGPKMQAAHATQGAAVIAIMQALLNAIFIVFAAASLSWLYRRYASELLTHAEDAPQ
                     "
BASE COUNT         1163 a         1817 c         1941 g         1015 t
ORIGIN      
        1 ggggacgtgg aggacggcat gtcggcgata gcgttcataa aatggtgcgg attccgttca
       61 tgccatgaag cgcgcgatgc gggtttcggc ccacatcccc acgcgcgaag tcagttcgac
      121 cagcgcgagg taacacaggc acagcaccag cagcgtttcg acgaaagcga aggtggccga
      181 gccgatgccg gtcagcacga acgtgagttc gggcacggtg acgatggaca gcaccgcggt
      241 ttccttgctc agcacgatga tcagattggt cagcgcgggc acgatcagca ccagcatctg
      301 cggcacctga atgcgcagga cggtctgcca gcgagtgagt ccgagacagg acgccgcttc
      361 gagatgaccc ggcggcaccg attgaaagcc cgagcggaat gcttcggcga aatacgcgct
      421 gccgtagagg cctaagccga gcacaccggc cgtcatcggt tcgagcgtga ggccgaagga
      481 aggaccgccg tagtacagca aaaacagttg cacgagaaac ggcgtgccgc gaaacagttc
      541 gatatagaag cgcaatatgc cgcgcaccca gcgtccgcaa aagagttgca gcacggcgat
      601 cagaaagccg atcagcatgc cgatcgccac gccggcggcc caggtgccga gcgtggtcgc
      661 cagaccggcg gcgatgggct gcaggttgtg ggtgatgacg gtggggtcga gcttttgcat
      721 gactgggtcg atctcgagct caagggcgct caggcgtgct gcaaacggta ttcggccgca
      781 tgcgcgacga gcgccaacgt gccgcagatc acgaagtaga tcagcccggc cgcgacgtag
      841 gcttcgagcg gccgataggt gctggccgcg atgttctgcg ccgtacgcgt caactcggcg
      901 acacccacta ccgagatcag cgacgaagcc ttgatcagca gcaccatttc attgacgagc
      961 gagggcagcg tcaaccggaa cgcctgcggc accagaatgc gccgcagcat atcgaacggt
     1021 gcaagcccga gcatgcgcgc ggcttcgatc tgacccggcg caatactgag gaagccgcca
     1081 cgcagaatct cggcgatata cgaagccgaa cacagcgaca cagcgctgag tgccgcgacg
     1141 atgggcgata cattgattcc ggcgaacggc agcaggtagt acaccagcag caattgcacc
     1201 agcatcggca cgccacgaaa gaagaacacg taagcgccgc cgaacatgcg cagcgagcgc
     1261 ttcggggaga ggcgtgccgc acacacgccg atcgcgacga agaagccgat tacaaggccc
     1321 gtcagcgaaa tgccgatggt ggcgagcgcc gcatgcagca gcagcggcaa gccctggaga
     1381 aagacggttg cgctgaacat cttgtgctga acatcaatag ttcggcgcgg gcatcgtggt
     1441 cggcgcatcc atcgcgacgc cgaaccactt tttctgcaag gccgcgaggc gcccgtcgtt
     1501 gtgcattttc acgagcgcgg cgttgaaggc atcggcgagc ggcttgctgt ccgcgtcttt
     1561 gcgcaggcag taagcaaagt acaccttcgc gccgaacggc ggttgcacca cggcgaaggt
     1621 ttcggggcgt tgcttggcca catacgcgat gttggtcatc gagttcgcga cggcggcgat
     1681 gcggccggcg gcgagatcgg cataagcctg attgttgtcg acgtattcgc ggatttcagg
     1741 cggtttcggc aaggtggcga cgtaggcctt cagctggtcg agctgggccg agcctttgcc
     1801 cgcgccgacc gttttgccga cgatatccga cgattgcttg acggaggtgt cgttggcgcg
     1861 cttgagcagc gcgtcggtgg cgtccgcgac cggcagggtg taggtgtagc gctccatgcg
     1921 cgccttggtg accgtcagcg ggccgcccac catatcgaac ttgccggctt cgagacccgg
     1981 cagcacgctt ggccacggca agtcgatgaa gcgcaccttc acacccattt ccttgccgac
     2041 ctcggcgaac agatccttat tgaagcccgc ttgctggccg ttttcaagga agtcgaaggg
     2101 cgcgaactgc atctcggtgc cgaccaccag ttcgcctgct tttttcactt tggcgagctg
     2161 gtcttccgca tgggcggtga cgcttgccgc gcacaacgcc agcatgacca aacgacactt
     2221 ccagcctgca aggatgctca tgggattctc cggttggcaa agcggtgtcg cgcgcaagcg
     2281 gttcggcagc gggcccggcg cgagtcatgc gaggcagtat ataccgcagt tttcgcgcga
     2341 aataaataat cgcgcgagta gagaaaagcc cttacgtgaa gcagcggcaa gccacttgcg
     2401 agtgcgcttt tggcggtata tacaacgggg atgagcgagg cacgcgagcg tgtcgcgcgt
     2461 gatttgcaag cgtcgatttg caaaccttga tttgcaggct tcactcatca gctgaattgg
     2521 caaacccctc cagggagagc aacgtcatgg ccgtgacccg cattccgatc atcgatctcg
     2581 ccggcgttcg cgcgggcgat ccccaggcgg tgacgcgcgt ggcgcacgaa atccgcgaag
     2641 catgcacgac gatcggcttc ttctacatcg tcaatcatgg cgttccgcaa gcagcgatcg
     2701 acgcagccga acaggcggca cgcaggttct ttgcgtttcc ggtggagacg aagcggcgcg
     2761 cggcggtcaa tcaaaggcat cggggtttca atgcgcttgg cgacgccacc atgtatcagg
     2821 ccaagcgccc cgactacaag gaattcttca gcatcggcct cgagttgccg gaggacgatc
     2881 ccgatgtgct cgccgggcag gcgctgcgtg ggccgaacaa ctggccggat ttcatgccgg
     2941 agctgcggcc cgcgctttat ggctactacg aagcggtcgc cgcgtgcggc gccgatctgc
     3001 tgcgggcagt ggcgctaagt cttggcgtcg gcgagcagtt cttcgcgccg cgttatacga
     3061 agcggatgca gcgcacgcag atggtctact atccgccgca gccgcctcag tcggacgcgg
     3121 atcagttcgg tgtcgcgccc cataccgact acggttgcat taccttgctg tggcaggatc
     3181 aggtgggcgg cttgcaggtg cgtgagatcg tcaacgacac gtgggtcgat gcgccacctg
     3241 tcgaaggcag cttcgtggtg aatgtcggcg atctgctggc gcgctggacc aatgaccgct
     3301 ttcgctccac cttgcatcgc gtgatcaatg cgtcggggcg cgagcgctat tcgatcgcga
     3361 cgttctatga tcccacttac ggtgcgatgg ttgatccgcg tgagctcggt acgagcgacg
     3421 cggacctcag gtaccaaccg gtcgccgcgg gtgactatat cctcgggcga atcaacgatt
     3481 cgatgggtta tcggaagaaa gcggccgttg caggcacgca aggaactact gcatgacaga
     3541 tcacagttcg gcaaacgcac gcagcagcgc aatccacagt ccagcgccgc tttccgcgac
     3601 actcgcggcg ctgcgtgccg cgctcggtga cgacgcggtg cgggtcggcg agcagatcgg
     3661 cgaacgcgcg atgaccgatt ggacacgtca cgatccgacg cgtcccgctg cattgctctt
     3721 accgcgcagc acggaagaag tctcgcgcgc gctggccatt tgccacggcg cgcatcaacc
     3781 ggtggtgccg caaggcggca tgaccgggct cgccggcggc gcgattgcgc gtgccaccga
     3841 tatcgcgctg tcgctcgaac ggctcaccgg cgtcgaggaa atcgacaccg cctcggccac
     3901 cctgaccgta cgcgccggca ccacgttgca aaccgcgcag gaagccgccg ccgaagcggg
     3961 cttcgaactc gcgctcgatc ttggcgcgcg cggctcatgc cagatcggcg gcaatctggc
     4021 gaccaacgca ggcggcaatc gcgtgatcca gtcaggcacc gcgcgcgacc aggtacttgg
     4081 ccttgaagtg gtgctcgcca atggcgacgt gctgacttcg ctcggcaaga tggtcaagaa
     4141 caatacgggc tacgacctga agcattggtt catcggctcg gaaggcacgc tcggcgtgat
     4201 cacgcgcgcg gtgctgcggc tgcatccgca acgcgcggcg cggcacacgg cgctcgtcgc
     4261 gctcgacggt tacgaggccg ccgtgagcct gctgcgccgc ctgtcgacgc gctttggcaa
     4321 cgacatcggc gcgttcgaaa tcatgtggcc ggatttctac gatttcggcg tgaagctgac
     4381 gggcacgcgc tcgccgttcg cggcgtcgca cccgctctat gcactgatcg aacacgcgag
     4441 tttcgacgcc ggcgacgacg gcgaacgttt ctccgccgca ctgaccgatg cactcgactc
     4501 ggcagcgatt cgcgatgcgg tgatcgcgca atcggtggcc gacgtacggg cgttatgggc
     4561 gattcgcgaa tgcaccgcgg agtttccggt gcggctcgat gcgatcaact tcgacgtgag
     4621 cttgccgatc agcgggatcg gcgctttcgt cgaccgttgc cgcgcggcgc tcgatcaacg
     4681 ctggccgggc aatgcgtcgt atttcttcgg ccatatcggc gattcgaatc tgcatgtcac
     4741 cgttgacggc cattctattc ccggcgtcga ccatcacgcg gtgtatgcgt tcgtctatga
     4801 gatgctgggg ccgttgcacg ggtcggtgtc ggctgaacac ggaatcggct tgctcaagcg
     4861 cgagttcttg ccgatctcgc gctcgcctgc tgaattggcc gcgatggccg cgatcaaaca
     4921 tgcgctggac ccccacggca ttctgaatcc gggcaagctg ttttgagcaa cgcgtgcggg
     4981 ccgcggtggg gtccggagat gtgaggcacg tagatgcgag gtccgcagaa gcgaggcccg
     5041 cggaggcgag gcacgcagca caacgccgcc acgcacacag cgcggaactc gctgccgcgc
     5101 gatttactgt ggtgcgtctt cggcgtgcgt cagtagctcg ctggcgtaac gccggtacaa
     5161 ccatgacagc gaggccgcgg cgaacacaat gaagatggca ttgagcagcg cctgcatgat
     5221 cgcgatgaca gccgcgccct gggtcgcgtg cgcggcctgc atcttagggc ccgcgatgaa
     5281 gaggaaaatg gcgctcagca cgaagaccgg cagatacgtg acgaagccca cggcgatcat
     5341 gctccacaca tgcccgcggc tgtctttcca ggcggcgcgc agcacgagcc tgctgccgag
     5401 cgcaatggcc ggatagagca gaatcagccg catggtgacg aacagataga tcgccagcat
     5461 gggcaggtag atcaggacgc ccagccgcac cgtgcgcatg gcgacgaaac ccaggccggc
     5521 aaacaccagc atcgtcagca cgacggcaag cgagaccagc gcgtagcggc cgagcggctt
     5581 gccattgagc ggtacgagcg gctgcacgcc ttcgccgagc agcacgaaac gatggacctt
     5641 gatggtcagc gtgccaccca cggccaattg cagcgcggtc cagaccagcg gaagcagccg
     5701 ctggccgaga tttgccatcg cgtcgacgcc gctggcgggc gtgacggcgg cagggtgcgg
     5761 gccggccagc agcgacgtgc aggccagtac cgcgcaaacg cccaggaaca accagggcat
     5821 ttgcgtaacg gcttgccacg aactgatcca gcctttcttg acgcaggccc cgaaggtcaa
     5881 ctgttccata gtttttagtg aaatcgactt gatgggtgac gccccttgac tttgca
//
LOCUS       DATASH010000061         1723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2776644,
            whole genome shotgun sequence.
ACCESSION   DATASH010000061 DATASH010000000
VERSION     DATASH010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2776644"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(227..493)
                     /locus_tag="VHV99_01830"
     CDS             complement(227..493)
                     /locus_tag="VHV99_01830"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEX3634229.1"
                     /translation="MRDGDISEEKKGNPPRKKSAALESLVQVESLIQLALVLPAATVV
                     GLLIGVGLDKWLHQHWIYIAGMIVGAAAGFVQIFRTVLALSKAN"
     gene            653..>1723
                     /locus_tag="VHV99_01835"
     CDS             653..>1723
                     /locus_tag="VHV99_01835"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEX3634230.1"
                     /translation="MFESEFERSLFALRQEKLQQIAALGHAAYPNHYRTTHEIPALRA
                     RFDSWTAEQLEAERTQVSVAGRIMAIRAQGKAGFAQLQQGGQRLQIYVRLDAVGEQAF
                     QLYKLLDLGDHIGVTGYLFRTRTGELTVHVDTITFLTKSMLALPEKYHGLEDVELRYR
                     QRYLDLFINTDVRAVFVKRAQILRAVRSFFDERGYMEVETPMMQSIAGGAMARPFTTH
                     HTALDIPLFLRIAPELYLKRLVVGGLDRVYEINRNFRNEGVSTQHNPEFTMLEFYQAY
                     ANYHDLMDLTEELVKFVAMEVNGTLTTEFNGTTIDLAQWKRYSMREAIAAFWPSNAGA
                     APTVANFATEASVHELIARARAA"
BASE COUNT          337 a          599 c          471 g          316 t
ORIGIN      
        1 cccaccgcga acatggccgc cgactgccac cccgccgcgc gccagatcgc aagcgcgccc
       61 agcaccccaa agatgcccac ccagcgcagc gcacgcatca gtgtcgcctt caggtcgcgc
      121 tcggtgaagt ccgggagtgg cgaggcattc ggttcagtca agattcggtc acgtctcttc
      181 ttgagattgt ttcagaaatg caggccaggc gatcgcgccg gccctgttag ttggctttgc
      241 tcaatgccag caccgtgcga aagatctgca caaatccggc ggccgcacca acgatcatcc
      301 cagcaatgta gatccaatgc tgatgcagcc acttgtccaa ccccacgcca atcagcagac
      361 cgaccaccgt agccgccggc aacactagcg ccagttgaat caggctctcc acctgcacca
      421 gcgactcgag cgcggcgctc tttttccgcg gcgggttgcc cttcttttcc tcgctgatgt
      481 cgccgtccct catgctgact gctcgagcat tttcactaca gggtatgcaa gagcgccacg
      541 gttcgcggag ctcgaccgta ctcaatagca gtgaaacgct ctcgatcctg cgctgttata
      601 caccatcgtc tcggtatact gaccgaacgg tatctccctc aaaggaaaca gagtgttcga
      661 gtcagagttc gagcgcagcc tgttcgcgct gcgccaggaa aaactgcagc agatcgctgc
      721 gctgggccac gccgcctacc ccaatcacta ccgcaccacg cacgagatcc ccgcactgcg
      781 ggcgcgcttc gatagctgga cagcggagca gcttgaggcc gaacgcaccc aggtctccgt
      841 cgctggccgc atcatggcca tccgcgccca gggcaaggcc ggctttgcgc agttgcagca
      901 gggcggtcag cgcctgcaga tttacgttcg cctcgacgcc gtgggcgaac aagccttcca
      961 gctttacaag ctgctcgatc tcggcgacca catcggggtc actggctatc tcttccgcac
     1021 ccgcaccggc gagctcaccg tgcatgtgga caccatcacc ttcctcacca agtccatgct
     1081 cgcgctgccg gagaagtatc acggcctcga agatgtggag ctgcgctacc gccagcgcta
     1141 tctcgatctc ttcatcaaca ccgacgtccg agcggtcttc gtcaaacgcg cgcagattct
     1201 gcgtgcggtg cgcagcttct tcgatgagcg cggctacatg gaagtcgaga cgccgatgat
     1261 gcagtccatt gccggcggcg caatggcgcg ccctttcacc acgcaccaca ccgcgctgga
     1321 catcccgctc tttctgcgta tcgcgccgga gctttacctg aagcgcctgg tggtcggcgg
     1381 cctcgaccgc gtctacgaga tcaatcgcaa cttccgcaac gagggcgtct ccacgcaaca
     1441 caaccccgag ttcaccatgc tcgagttcta ccaggcctac gcgaactacc acgacctgat
     1501 ggacctgacc gaagagctgg tgaagtttgt cgcgatggag gtgaacggca cgctgaccac
     1561 tgagttcaac ggcaccacca tcgatctcgc ccagtggaag cgctactcca tgcgcgaggc
     1621 catcgccgcc ttctggccat ccaacgcggg cgcagctccc acagtcgcga acttcgcgac
     1681 agaggcgtcc gtgcatgagc tgattgcgcg cgcgcgtgcg gct
//
LOCUS       DATASH010000062         7111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3963825,
            whole genome shotgun sequence.
ACCESSION   DATASH010000062 DATASH010000000
VERSION     DATASH010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7111
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3963825"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1592
                     /locus_tag="VHV99_01840"
     CDS             <1..1592
                     /locus_tag="VHV99_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HEX3634231.1"
                     /translation="GTILQILINEMDFLTNGPMGVKIPKPSIGGRPMNEVEYYWLVAA
                     LLVVSLIVVHRVLKSHLGRAFEALRDSPIASDCMGVSVYRYKVYAFVISAGFAGLAGC
                     LYSYSEQYISPNTYNFELTILFLLAIIMGGRKTRTGALLGSAIIVLLPKLLDDIDMFR
                     IVASVLAAVVVIGAAVALARKVSAPRKVAIPVLGTVGLAAFSYRIDTLADWRLTIFGL
                     MILLVVYYLQDGVVGFVRKIVTHGRVRTVSVDTTKPPAIGADAIQAVHAGGATGNILE
                     LRGILMQFSGLKALNNVDLIVRRGTIHGLIGPNGSGKSTMMNVLTGIYVPTAGTLEYR
                     GQSLAGKTSSQIALSGIARTFQNVQLFGEMTALENVLVGLHHTFKTNLADVGLMSARY
                     KREEHAARERAFGMLRFVGLENVAGEEARNLPYGKQRLLEIARALALDPQLLLLDEPA
                     AGLTAPDIKELVAIIRKVRDHGITVVLIEHHMDVVMSVCDRVSVLDFGQKIAEGKPAD
                     IQSDEKVIEAYLGGQAAGQAA"
     gene            1622..2356
                     /locus_tag="VHV99_01845"
     CDS             1622..2356
                     /locus_tag="VHV99_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3634232.1"
                     /translation="MLSIRNLHAGYGKVKVLHGISIDVPKASVVTLIGSNGAGKTTTM
                     RAISGMIRPTAGEITMGVGVDAKRIDALDSHRIARLGLAHSPEGRRVFATMSVTDNLL
                     LGAFPRLTWARPRGDVNADLERAIELFPRLKERRHQLAGTLSGGEQQMLAMARAVMLN
                     PDIVLLDEPSMGLAPILVEEVFRIIGNLKSEGVTMLLVEQFAAAALNVADYGYVLENG
                     RIAAHGPAAELRNDPSVKAAYLGASH"
     gene            complement(2410..2592)
                     /locus_tag="VHV99_01850"
                     /pseudo
     CDS             complement(2410..2592)
                     /locus_tag="VHV99_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808419.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            2637..2844
                     /locus_tag="VHV99_01855"
                     /pseudo
     CDS             2637..2844
                     /locus_tag="VHV99_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176611.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding domain containing protein"
     gene            complement(2972..4324)
                     /locus_tag="VHV99_01860"
     CDS             complement(2972..4324)
                     /locus_tag="VHV99_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010119621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HEX3634233.1"
                     /translation="MARRPQTQRLDIWMNGLPVGYWEKNREGDRLSYLDEWIEDEQGR
                     PLSLSMPFTPGNQPYRGDVVSAFFDNLLPDSDAIRRRLAQRHRTGSTNAFDLLVALGR
                     DCVGAIQLLAPDEQPTDLYDISGTPLSDQQVARLLRNTTSAAPLGQYDSGADLRLSIA
                     GAQEKTALLRHDKQWIYPTGSTPTTHIFKLPLGLVGNMQADMRTSIENEWLCSKIVAA
                     YGLPAADCEIATFEDQKALVVERFDRRLSSDASWIVRLPQEDMCQATGRPSHLKYQAD
                     GGPGIAEIMDVLLGSENADQDRRQFFKTQLVFWLLAATDGHAKNFSIFHLPHGLYRST
                     PLYDVLSAHPIIGPGRNQIARQKAKMAMAVRGSTNHYLIDKIQRRHWIEQARQVELGS
                     ETAQDIIAEVVDSTDHVINEVSGLVPSGFPKGLAESILSGVGRQRNKLAVAPSRSAAG
                     "
     gene            complement(4327..4722)
                     /locus_tag="VHV99_01865"
     CDS             complement(4327..4722)
                     /locus_tag="VHV99_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012404769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX3634234.1"
                     /translation="MDYAIKTLSQLRPILLGFRKSAGLTQAAVAELLGITQQSYAQME
                     ANPASASVERLFKVMRLMNVELRMSQASSAPGSQPAQARTPKRQQPAPTVKKAKPPAA
                     TNRSGRTQKASTSKQPLGAITAKKKRENW"
     gene            4982..5593
                     /locus_tag="VHV99_01870"
     CDS             4982..5593
                     /locus_tag="VHV99_01870"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX3634235.1"
                     /translation="MSHYFYDPAAGHGLPHDPFKAIVAPRLIGWISSRDTNGTLNLAP
                     YSFFGAFASFPPIIGFCSEGRKDSVANIEATGEFVWNLATKPLAEQMNRSSAPVPPHV
                     DEFELAGLTPAPGRNVNVPHVAESPAALECKLLQIVRLHTLDGKPMDNYLSLGQVVGV
                     HINEAYLKDGLFDTHAAQPIMRAGYRADYAEIGEMFQMFRPSA"
     gene            complement(5797..>7111)
                     /gene="phaZ"
                     /locus_tag="VHV99_01875"
     CDS             complement(5797..>7111)
                     /gene="phaZ"
                     /locus_tag="VHV99_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HEX3634236.1"
                     /translation="FGDTSHGRSLRAACELLTLAGLTPVRPPFDIDSIVTEGKPVKIV
                     EEVAARTPFCSLLHFRKDATLSHPQPRVLVIAPMSGHFATLLRGTVRTMLAEHDVYIT
                     DWHNPRDVPLSLGRFGFNEFVQHVIDFTEAIGPGAHLLAVCQPTVAALAAVALMAADD
                     NPAQPASMTLMAGPLDTRINPTRVNELAKSRPIEWFEQNLISAVPFGFAGAHRRVYPG
                     FVQLTAFMSMNLSRHLDSFETLYYERAKGDPVKADAIHTFYEEYFATMDLTADFYLET
                     VDTVFQRHALPLHELEVEGRLVEPSKIRRTALLTVEGEKDDICAVGQTLAAQDMCDKL
                     RPYLKTHHVQTGVGHYGVFNGHRWERQIYPRVRAVIYDNEPRAVLVSSRARTIQPVSV
                     AVASELMAAAPAAAAVGDVEMDTNVTTATPNRATPGDVAKPQQAS"
BASE COUNT         1330 a         2143 c         2229 g         1409 t
ORIGIN      
        1 tcggcacgat cctccagatc ctcatcaacg agatggattt tctgaccaac gggccgatgg
       61 gcgtgaagat tccgaagccg tcgatcggcg ggcggccgat gaacgaggtc gaatattact
      121 ggctcgtcgc ggcgctgctg gtggtgtcgc tgatcgtcgt gcatcgtgtg ctcaagtcgc
      181 atctgggccg cgcgttcgag gcgctgcgcg acagtccgat cgcgtcggac tgcatgggcg
      241 tttccgtgta ccgctacaag gtgtacgcgt ttgtgatcag cgcgggcttc gcggggctcg
      301 ccggttgtct gtactcgtat tcggagcagt acatttcgcc gaacacgtat aacttcgaac
      361 tgaccatcct gttcctgctc gcgatcatca tgggcgggcg caagacgcgc accggcgcgt
      421 tgctcggctc ggcgatcatc gtgctgctgc cgaaactgct cgacgatatc gacatgttcc
      481 gcatcgtcgc ctcggtgctg gcggcggtcg tcgtgatcgg cgcggctgtg gcgctggcgc
      541 gcaaggtgtc ggcgccgcgc aaggttgcga ttccagtact cggcacggtg ggactcgctg
      601 ccttctcata tcggatcgat acgctcgccg actggcggct gacgatcttc gggctgatga
      661 ttctgctggt ggtgtattac ctgcaggacg gcgtggtcgg attcgtgcgc aagatcgtga
      721 cgcacggccg ggttcgcacg gtgagcgtcg atacgacgaa accgccggcg atcggcgctg
      781 acgcgattca ggcggtgcat gcgggcggcg cgaccgggaa catccttgaa ttgcgcggca
      841 ttctgatgca attcagcgga ctgaaggcac tcaacaacgt cgacctgatt gtgcggcgtg
      901 ggacgattca cggcttgatc ggaccgaacg gctccggcaa gagcacgatg atgaacgtgc
      961 tcaccggcat ctacgtacca accgccggca cgctcgagta tcgcggccag tcgctcgcgg
     1021 ggaagacgtc gtcgcagatc gcgttgtcgg gtatcgcgcg cacgttccag aacgtccagt
     1081 tgttcggcga aatgaccgcg ctcgagaacg tgctggtcgg tctacatcac acgttcaaga
     1141 cgaacctcgc cgatgtggga ttgatgtcgg cgcgctacaa gcgcgaagag cacgccgccc
     1201 gtgagcgtgc gttcggcatg ctgcgtttcg tggggctgga aaacgtcgcc ggggaagaag
     1261 cgcgcaacct gccgtacggc aagcaacggc tgctcgaaat tgcgcgtgcg ctcgcgctcg
     1321 acccgcaact gctgctgctc gacgaacccg ccgcggggct taccgcgccg gatatcaagg
     1381 agctcgtcgc gatcatccgc aaggtgcgcg atcacggcat taccgttgtg ctgatcgagc
     1441 atcacatgga cgtggtgatg agcgtgtgcg accgcgtctc cgtgctggac ttcgggcaga
     1501 agatcgccga aggcaagccc gccgacatcc agtcggacga gaaagtgatc gaggcttatc
     1561 tcggcggcca ggctgccggc caggctgcgt gaagacgctc acgtctccca caggaacaga
     1621 catgctatcg atcagaaatc tgcatgcggg ttacggcaag gtcaaagtgc tgcatggcat
     1681 ttcgatcgac gtgccgaagg cgtcggtcgt cacgttgatc ggctcgaacg gcgcgggcaa
     1741 gaccacgacg atgcgcgcga tctccggcat gatccgcccc actgccggcg agatcacgat
     1801 gggtgtgggc gtcgacgcga agcgcatcga cgcgctggat tcgcaccgca tcgcgcggct
     1861 cgggctcgcg cattcgccgg aaggccggcg cgtgttcgcg acgatgagcg tcaccgacaa
     1921 tctgcttctc ggcgctttcc cgcgtctcac gtgggcgagg ccgcgcggcg atgtgaatgc
     1981 ggatctggag cgggcgatcg aactatttcc gcggctgaag gagcgccggc atcaactggc
     2041 cggcacgctc tccggcggcg agcagcaaat gctggcgatg gcgcgcgcgg tgatgctgaa
     2101 tccggatatc gtgctgctcg acgaaccgtc gatggggctc gcaccgattc tcgtcgagga
     2161 agtgttccgg attatcggca atctgaagag cgaaggtgtg acgatgctgc tggttgagca
     2221 gtttgcggcg gcagcgctta atgtggctga ttatgggtac gtgctggaga acggcaggat
     2281 cgcggcgcat ggtccagcgg cggagttgcg taatgatcct tcggtcaagg cggcttatct
     2341 gggggctagt cattgagggg gcattgatga cccgctatag acgacgtttt tcagcttttg
     2401 ccgccgacct cacgcgacca gcagatagcc gagcacgacg tctgtaatgt gttgctgccg
     2461 ttccatatag gcgcgccgcg tattcaggtc gcgcccaaac gacatggaaa gggtgtagtt
     2521 gttcgaaagg tagaagtatc ccaagccggc aatcgatata tagagttgca gcgggtccac
     2581 gccgccgcgg aaaagcgatc ggaacgcctc gcgagccgac tcaggttgac gcagacgcga
     2641 aggccgagcc agttctgatc tatgcgagct gaacgcccga tgaagtcaag gcggcgcagc
     2701 gcgagttcgg cgtcgcgcag gcagggacag tcatggaggc ggcgctggtc accatcgctc
     2761 gtgatctgcg agatgctggc ttcaggaagt tcgtggttgc gggcgggggg ctcaggtgcc
     2821 ggcgtgcagg cgcttgaggt caatgcgtgg tatcaggcgg agcctggttc aagtcgggca
     2881 gcggcgaagc ccatttggcg caagtcaagg aaggccgtaa gcgggtgatt cgtcgcggtt
     2941 aatggcgcca ggcaggaggc actttcctct gctacccagc cgccgatcga ctcggcgcca
     3001 ccgctagctt atttcgctgc ctcccgactc cactcagaat cgattccgcc agtcctttcg
     3061 gaaatcctga cggcacgaga ccgctcacct cgttgatcac gtgatcggtc gagtcgacta
     3121 cttcggcgat gatgtcctgc gccgtctcgc ttcccagctc aacttgccga gcctgttcga
     3181 tccagtgccg gcgctgaatc ttgtcgatca gatagtgatt cgtactgccg cggacggcca
     3241 tcgccatctt cgccttctgg cgcgcaatct gatttcgccc cgggccgatg atcggatggg
     3301 cggagagcac gtcgtaaagg ggtgtcgagc ggtataagcc gtgaggtaaa tgaaaaatgc
     3361 tgaagttctt cgcgtgccca tcggttgccg ccagtagcca gaacacgagt tgagttttga
     3421 agaactgccg ccgatcctga tccgcgtttt ccgagccgag caatacgtcc atgatctcgg
     3481 cgatgcctgg cccgccgtca gcctggtact tcaggtgcga gggccttccg gtggcctggc
     3541 acatgtcttc ctgaggaagg cgcacgatcc agctcgcgtc actggacagc ctacggtcga
     3601 agcgctcgac cacgagcgct ttctgatcct cgaacgttgc aatctcgcaa tccgccgcag
     3661 gcagtccata cgcggctacg attttcgagc acagccattc gttttcgatc gacgtccgca
     3721 tatccgcctg catgttccca acgaggccga gcgggagctt gaagatatgc gtggtcggcg
     3781 tgctgccggt cgggtagatc cactgcttat catggcgcag cagcgccgtt ttctcttgcg
     3841 caccagcgat cgataacctc agatccgcac cgctgtcgta ttggccgagc ggcgctgctg
     3901 aggtggtgtt gcgcagcaac cgcgcgacct gctgatcgga tagcggtgtt ccagagatgt
     3961 cgtaaagatc ggtgggttgc tcatcaggcg caaggagctg gatcgcgccg acgcagtcac
     4021 ggccaagtgc caccagcaga tcaaaagcat tcgtgctgcc cgtgcgatgc cgttgagcca
     4081 atcgccgacg gatcgcatcg ctatcgggaa gcagattgtc gaagaatgcg ctgactacgt
     4141 ccccacggta cggttgattt ccgggcgtga atggcatcga cagagaaagg ggacgcccct
     4201 gttcatcctc gatccactcg tcgagataac tcaggcggtc accttcccgg tttttttccc
     4261 agtagccgac gggcaggccg ttcatccaga tgtcgagtcg ctgcgtctga gggcggcgcg
     4321 ccatgattac cagttttccc ttttcttttt ggccgtgatt gccccgagag gctgtttcga
     4381 agtcgacgct ttctgagttc gcccggagcg gttggtggcc gccgggggct tcgctttctt
     4441 gacagtgggg gcgggttgtt ggcgctttgg cgtgcgggcc tgcgcgggtt gactgcctgg
     4501 tgcggacgac gcctggctca tgcgcagttc cacgttcatc aaacgcatga ccttaaaaag
     4561 ccgttcgaca ctcgccgatg ccggattggc ttccatttgt gcgtagcttt gctgggtgat
     4621 gcctagcagc tcggccaccg cagcctgtgt gagccctgcg gatttgcgga agcccaacag
     4681 aatggggcga agctgactta gtgtcttgat agcgtaatcc attggatttc ttcagactcg
     4741 tacagtctct gaactgtatc acacaaatac agttcagaga ctgtaaagca tatatacagg
     4801 ctatagactg tatttgaaat ttacagtccc aggcctgtcg agcggggaac ccgaagccaa
     4861 tcgatcaacc tgaatctgga agggccaata ccaggagcac ctttcaatcc tgtcccagca
     4921 gcgccagcga gtctgcggta taaatacctc ccgcctcgtc cgccaacccc aacgtaaaac
     4981 catgtcccac tatttctacg atcccgctgc cggtcatggc ctgccgcacg acccgttcaa
     5041 ggccatcgtc gccccgcgtc tgatcggctg gatttcctca cgcgacacca acggcacgct
     5101 gaacctcgcg ccttacagtt ttttcggcgc gttcgccagc ttcccgccga tcatcggatt
     5161 ctgcagcgaa ggccgtaaag acagcgtcgc caatatcgaa gcgacgggcg aattcgtctg
     5221 gaatctcgcc accaagccgc tcgccgagca aatgaaccgc tcgtccgcgc cggtcccgcc
     5281 gcacgtcgac gaattcgaac tcgccggcct cacgcctgcg ccgggtcgca atgtcaatgt
     5341 gccgcacgtc gccgaatcgc cggccgcgct cgaatgcaag ctgctgcaaa tcgtgcggtt
     5401 gcatacgctc gacggcaagc caatggacaa ctatctgtcc ctcggacaag tggtcggcgt
     5461 tcacatcaac gaggcctatt tgaaagacgg cctgttcgac acgcacgccg cgcagccgat
     5521 catgcgcgcc ggctaccgcg cggattacgc ggaaatcggc gagatgttcc agatgtttcg
     5581 cccgagcgca taacagtccg taaaaagcag aggcgtttga agctggacgc gccgggtagt
     5641 gcgtggcgcg gccttagcct ttggtgacgt gccggcggtg cagccgtgga cgagcgtcgc
     5701 ggcttgatcg ccgctgtgta aacccgcggc gcctcgcaca acgcggccgt ccaaccgaac
     5761 agctcattgc gccgccggcg gccgtcggcg tggcgattac gacgcttgct gtggcttggc
     5821 gacgtcgccg ggcgtggctc ggttcggcgt ggcagtcgtc acgttggtat ccatctcgac
     5881 gtcgcccacc gccgccgcag ccggcgctgc cgccatcagc tcagaagcta cggccaccga
     5941 caccggctgg atcgtgcgcg cccgcgaact caccagcacc gcgcgcggct cgttgtcgta
     6001 aatcacggct cgtacgcgtg gatagatctg ccgctcccac cgatgcccat tgaacacgcc
     6061 atagtgcccc acaccggttt gcacgtgatg cgtcttcaga tacggccgca acttgtcgca
     6121 catgtcctgc gcggcgagcg tctgccccac cgcgcagata tcgtcctttt ctccttcgac
     6181 agtgagcaac gcggtgcgac gaatctttga aggctccaca agacgccctt ctacctcgag
     6241 ctcatgcaac ggcagcgcat gccgctgaaa aaccgtatca accgtctcca gatagaagtc
     6301 ggccgtcagg tccatcgtcg cgaagtactc ttcatagaaa gtgtggatcg cgtcggcctt
     6361 gaccgggtcg cctttggctc gctcgtaata cagggtctca aaagagtcga gatggcggct
     6421 gaggttcatc gacataaacg cggtcaactg cacgaagccg ggatacacgc gacgatgcgc
     6481 gcccgcaaaa ccgaacggca cagcgctaat caggttctgc tcgaaccact cgatcggcct
     6541 gctcttcgcg agctcgttga cgcgcgtcgg gttgatgcgc gtgtcgagcg ggcccgccat
     6601 cagcgtcatg ctcgcgggct gcgcgggatt gtcatcggcg gccattagcg cgaccgcagc
     6661 caaagcggcg acggtcggct gacacaccgc cagtagatgc gcgccgggtc caatggcctc
     6721 ggtgaagtcg atcacgtgct gcacgaattc gttgaagcca aaacggccca ggcttaacgg
     6781 cacatcacgc gggttgtgcc aatcggtgat gtagacgtcg tgctcggcca gcatcgtgcg
     6841 cacggtgccg cgcaacagcg tggcgaagtg gccggacatg ggcgcgatca ccaacacgcg
     6901 cggctgcgga tgcgaaagcg tggcgtcctt acgaaaatgc aacagcgaac agaacggtgt
     6961 gcgcgcggcc acttcctcga cgatcttcac cggtttgcct tccgtcacta tgctatcgat
     7021 gtcgaacggc ggcctgacag gcgtgagtcc cgcgagcgtc aacagctcgc aggccgcgcg
     7081 tagcgaacgt ccatgcgacg tatcgccgaa t
//
LOCUS       DATASH010000063         1630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3173278,
            whole genome shotgun sequence.
ACCESSION   DATASH010000063 DATASH010000000
VERSION     DATASH010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3173278"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            290..>1630
                     /locus_tag="VHV99_01880"
     CDS             290..>1630
                     /locus_tag="VHV99_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX3634237.1"
                     /translation="MQEHPYAEARARDATLAAAPYGMFICAVDGTFDSVNAAFEKLTG
                     FAAEELIGRRSFESLHDPAELARRRTELPPLSAISTRYEGEWTYVRRNGTPIRVVFAL
                     APMVASIAAESAPPGGVAAQPARYVGIAIDMTRYAQSEARLWYVAHHDGVTRLPNQTL
                     FTERLELTIARCQRNGNGFTVLIAELDHLRKLRDALGLHAAELVLQIVGERMRGLFPN
                     DGTIASIGGTQFALLINETGAAADAFAAEALGRIAEAIDYAGTTLNITASIGLVAYPA
                     DGADAPTLMRRAGVALSAASAVNGNAVRRFSTALEGQAARRFELETMLREALERQQLH
                     LVYQPQVTLANGRIAQVETLLRWNHPQRGLISPAEFVPVAEEAGLIEQIGEWVIRTAC
                     RDAGKLLRLTGTLPRIAVNVSPQQFQRHNLFETIREALEDAALDPSYLELEITEG"
BASE COUNT          294 a          505 c          538 g          293 t
ORIGIN      
        1 caggcccgtg cgcgttgagt catgagacgc ggagtatccc ggccatagcg aacgcttttg
       61 atgggtgcga tagcggacgt gctgccgccg gagtggggga atacgccgag taaaacgatt
      121 gcgcgtggag aatgcgcggc cggcgcggcg gggcggccgg aaaagccgct atcatacgca
      181 cggttagcgg gcggatcgga ccggatctgc ccgcgtcggc gcttgcgtcg tacgcgccag
      241 aaaaaacgct cactcgcttt caatatcaga aaattcaaag gccggtgcga tgcaagaaca
      301 cccctatgcc gaagcgcgcg cacgcgacgc cacgcttgcg gccgcgccat acggcatgtt
      361 catctgcgcg gtcgacggca cgttcgactc ggtcaatgcg gctttcgaaa agctgactgg
      421 ctttgccgcc gaagagctga tcggccggcg ctcatttgaa tccttgcacg atccggccga
      481 actcgcgcgg cggcgcaccg aattaccgcc gctgtcggcg ataagcacgc gctatgaagg
      541 cgaatggacc tatgtgcggc gcaacggcac gcccattcgc gtggtgttcg cgctggcgcc
      601 gatggtggcg tcgattgccg ccgagtcggc gcctcctggc ggtgtcgccg ctcagcctgc
      661 ccgctatgtc ggcatcgcga tcgatatgac gcgctacgcg caatccgaag cgcgcttgtg
      721 gtacgtcgcg catcacgacg gtgtgacccg tctacccaat cagacgcttt tcactgaacg
      781 gctcgagctc acgatcgcgc gctgtcaacg gaacggcaat ggctttaccg tgctgatcgc
      841 cgagctcgat catttgcgca aactgcgcga tgcgctcggt ttgcatgcgg ccgagctggt
      901 gttgcagatc gtcggtgagc ggatgcgcgg actgttcccc aacgacggta cgatcgcctc
      961 gatcggcggc acgcaattcg cgctgctgat caatgaaacc ggcgctgcgg ccgatgcgtt
     1021 cgctgccgag gcactgggcc gcatcgccga agcgatcgac tacgctggca ccacgctgaa
     1081 catcacggcg agtatcggcc tcgtcgccta tcctgccgac ggtgccgatg caccgacgct
     1141 gatgcgtcgc gcgggggtgg cgttatcggc ggcttcggcg gtgaacggca atgcggtgcg
     1201 gcgtttttcc accgcgctag aaggtcaggc agcgcgccgc ttcgaactgg agacgatgct
     1261 gcgtgaagcg ctcgagcgtc agcaattgca tctcgtgtat caaccgcagg tgacgctcgc
     1321 caacgggcgc atcgcgcaag tggaaacgct gctgcgctgg aaccatccgc agcgtggctt
     1381 gatcagtccg gccgaattcg tgccggtcgc ggaagaggcg ggcctgattg agcagatcgg
     1441 cgaatgggtg attcgtaccg cgtgccgtga tgccggcaaa ctgctgcggc tgaccggtac
     1501 gttgccgcgg atcgcggtga acgtttcccc gcagcaattc caacggcaca acctgttcga
     1561 gacgatccgc gaggcgctcg aagacgcggc gctcgaccct tcctacctcg agctggaaat
     1621 caccgagggt
//
LOCUS       DATASH010000064         7072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2579738,
            whole genome shotgun sequence.
ACCESSION   DATASH010000064 DATASH010000000
VERSION     DATASH010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7072
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2579738"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(680..904)
                     /locus_tag="VHV99_01885"
     CDS             complement(680..904)
                     /locus_tag="VHV99_01885"
                     /inference="COORDINATES: protein motif:HMM:NF043076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhaM family polyhydroxyalkanoate granule
                     multifunctional regulatory protein"
                     /protein_id="HEX3634238.1"
                     /translation="MSEGAQKTLAFTLNMITPLLNVDNLDKRITDLHTVKQWLELNLS
                     LLQSTNGSGDVAQIGLAREAIQFGDADGRA"
     gene            complement(983..2035)
                     /locus_tag="VHV99_01890"
     CDS             complement(983..2035)
                     /locus_tag="VHV99_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011355646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadG family pilus assembly protein"
                     /protein_id="HEX3634239.1"
                     /translation="MRNRTFPPRNRAPHAAPRQRGSVALFFLLFLIALLCFGAFAINL
                     ARVSVVLNELQNDADSAALAGASALVVAGGNGSNWTQAQAAATSAVTLNRSDGQTLTT
                     GAVQAGYWNLTGSPAGMEATTIAPGVYDAPAVQVTVTRATNQNGGAIALFLGSLLNLL
                     SVPGSATAVAVAAAPSTVDAGGLFPVVLDQCVFDQYWDATTNQPRIDPSTGQPYEFLI
                     TNGQKYGSSCEAGQWTSFLENANNVPTIRNLILNGNQSPLSIGDSIWIEPGAKTTIYR
                     SVPTNVTVVLPVATQIDSKTYVPIVAFAAFHIDASVGGSEKYIQGHFVGGYTLTTAAG
                     GVGPTYGAYMAPRLAQ"
     gene            complement(2044..2499)
                     /locus_tag="VHV99_01895"
     CDS             complement(2044..2499)
                     /locus_tag="VHV99_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEX3634240.1"
                     /translation="MVNRSRRGAQARSAGLPRASRGAIALEFVLVFPFLLIVLFGIID
                     VSLLLCDKALITNAGREAARAGVVVRVPQLTPAQISNVALNYLQNSLVTGGTATTPTV
                     TVNQSAGTSPGSPLTITVSYQYQGLVLGSAFSSLTGPITVTAVTVMNYE"
     gene            complement(2514..3377)
                     /locus_tag="VHV99_01900"
     CDS             complement(2514..3377)
                     /locus_tag="VHV99_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3634241.1"
                     /translation="MKHFPVLLRTLTTTLPLACGLLAGCASGGSFNPRPVSLQHGTDG
                     TSDERLADSVLQAGDVELATTLFEKALKSNPNSLDAQLGLADSIYETGDLERARALYQ
                     QAALQLPARPAAQLGLARIALRQRRLDDAAALYRGLLAAQPDNPVISEGLGTVLDLQE
                     RHSEAQDVYRKALALHPEVEGLRVNLGLSLILQDKPREGTNVLLDVAGLADAPSQARQ
                     DLALAYGLLGNADAAKKILLVDLPPSSVDDNLRFYQSLRSSLDEYRAAAARADGTATP
                     AVSPGTKASRQ"
     gene            complement(3374..4246)
                     /locus_tag="VHV99_01905"
     CDS             complement(3374..4246)
                     /locus_tag="VHV99_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX3634242.1"
                     /translation="MWPRRTRRRIAQRAYHAAMGADTQHMPGAEEHATEMWQRLIRRF
                     ATLGESVPLGDAAQRNKLAEQLVRAGFRERRAVSVMIGLKLLAGALPALAAIVLGPHV
                     PRIGGYFVFRALMMGGAFIVGMILPELALGAMVSRRQKAIASCLPDALDLLVICTNAG
                     NSLVVGVKRVARELKTICPPLADEFSVTGDELQIGGDSAVALRRLAARIGLPSVRALV
                     TTLVQSQQYGTPITQSLRMLSRAERTAQMLALEEKAAKLSVKMTLPMMLFILPTVGLI
                     AAGPAVIRLVSIFK"
     gene            complement(4321..5298)
                     /locus_tag="VHV99_01910"
     CDS             complement(4321..5298)
                     /locus_tag="VHV99_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX3634243.1"
                     /translation="MTPADIVAAGAFFAIVLAGMIVSSLRDMARRRPKPRIQDRLSQI
                     QQMRVASQQPATRDTSGELFELPKDYGAIEAWFQQRVQRLRAVSGAGGIRLVFASGIV
                     GASIAFIIVRLSPLPGWSGPFVYGGTPVLAMGMTHRFLIVRFRTRFLTVFPDTLDLII
                     RAVRAGIPVVQAISTAGHEAEEPVRSTFRTMGDSLQLGADLKEVLDKAVERLQIADFS
                     FFAVCLLLQRETGGNLGETLENLSGIIRSRRDIRLKTSALTAEGRIASKIIAAVPFLI
                     TGLLYVINRPYVELLFHTRAGHKMLTLAVVLLVAGLSMIQKISKLDTSR"
     gene            complement(5295..6677)
                     /locus_tag="VHV99_01915"
     CDS             complement(5295..6677)
                     /locus_tag="VHV99_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006026647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HEX3634244.1"
                     /translation="MFGAKKRMPTDSLLADAHVRAEQPAAAPPAHPRMPTDGHEALIR
                     SDNFKKIRAVVFASMNMSAALMKTRDQVRTGIEEIAVDVATREQLNVTAAEQALIVSE
                     ILNDMFGVGPIEPLLADETVTDILVNGPDQVYAERHGRLELTPLKFRDNAHVVNVAQR
                     IAAAVGRRVDESSPMVDARLADGSRVNVVLPPIALRGASISIRKFSKRNITLARMAQQ
                     ANISPEMVRVLKIASACRLNIIISGGTGSGKTTLLNALSHHIDEHERIVTIEDAAELQ
                     LQQPHVVSLETRPENSEGLGAVSQRDLVRNALRMRPDRIILGETRGPEAFDVLQAMNT
                     GHDGSMTTIHANTPRDAITRLESMVMMANGNLPLTAIRRQIASAVHLILQIERMRDGV
                     RRVTRVTELAGMEGDVIITQDLFAFRYDASAYQEDVKGTFESSSLRPVFATRAAYYGL
                     ETTLMEAMRP"
     gene            complement(6677..>7072)
                     /locus_tag="VHV99_01920"
     CDS             complement(6677..>7072)
                     /locus_tag="VHV99_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HEX3634245.1"
                     /translation="EALDASQVVCIVADPSVHAARECVRLLRFAEDRTGEPVVSVLLN
                     NPLEPVADRVQPKDFQRALGRSVLHELPYDPKSLARAENLGEPIVARKKQPGFAAAIE
                     RIANGLTGRESSAAPLPWHARLMTPRRTP"
BASE COUNT         1313 a         2208 c         2181 g         1370 t
ORIGIN      
        1 cgactcgttc tggacgacga cgaattgcgc cttctgtacg tggccaccac ccgcgcccaa
       61 cacgtgctcg atgtctccgc gcttcgcgaa gaattgatcc ggctgttcgc ctcccgtgcg
      121 tgaaaccgcc gcgcatcgtg cgtcgccggt cgaccctctc cggccgttgg atctagcgcg
      181 acgtgaacgg cggcttccga ggtccagcgg tcattcgtgc acgcgtgctg tccgtcaagg
      241 catcggccaa tgctgacctt ctcccagcgg cactcttgag cctccgtcaa cgtccgctcc
      301 agggaaagtc gaacgctcat tttcaccccc ctatacgcag gtgcaactat tcgcggatcg
      361 tggtggggac ccgtgattga ttcagctgag tcccagcttc cggcgatgtc gataggcgta
      421 gatttggaca tagtccccgc tgcgcattga agtggttttc ccgctttagg aaagcgcctt
      481 ggcgaacacc cgattgagta actaaggtcc tgcagactgc ggcgaaagtc ggaggcgctg
      541 cgccgctggt agaaaccgtg cgatagcgag ccgggcgccc gggcggcctc tgtctcttgt
      601 ccggccagcg ccaatgcggt gcatggcgcc cgctggcatc gtccggggcc agtcgatgcg
      661 cgcgggcgtc gtcagacttc taagcgcgcc cgtctgcgtc gccaaactga atggcctcgc
      721 gagcgaggcc gatctgcgcc acgtcaccgc ttccattggt tgactgcagc aggctaaggt
      781 tgagttcgag ccactgcttg actgtgtgca ggtcggtgat ccgtttatcg agattgtcga
      841 cgttcaatag cggcgtaatc atgtttagcg tgaaagccag cgttttttgc gcgccctcag
      901 acaaatctga cgcgagcccg gtaagccgct gcctcagtct aagtggctgg gacaattcgt
      961 agccggcgct tggcgacggc cgttactgcg cgagccgcgg tgccatatac gctccgtagg
     1021 tcggtccgac gccacccgct gccgtggtca gtgtgtagcc accaacgaaa tgcccttgaa
     1081 tgtacttctc gctgccgccg accgatgcgt cgatgtggaa cgcggcaaaa gcgacgatcg
     1141 gaacatacgt tttgctgtcg atctgtgtgg cgacaggcag gacgaccgtc acattagtcg
     1201 gcaccgatct gtaaatggtg gtttttgcgc cgggttcgat ccagatgctg tcgccaatgc
     1261 tcagtggtga ctgattcccg ttaagaatca ggttgcgaat cgtcggcaca ttgttggcgt
     1321 tctcaagaaa cgacgtccat tggccggctt cacacgagct cccatacttc tgcccgttag
     1381 tgataagaaa ttcgtaaggc tggcccgtcg acggatcgat tcgtggttga ttcgtcgttg
     1441 cgtcccagta ttgatcgaag acgcattgat ccagtaccac gggaaacaag ccgcccgcat
     1501 cgaccgtgct aggcgccgcg gccaccgcca ccgcagtggc actgcccggc acgctaagca
     1561 ggttcagaag gcttcccaga aacagcgcga tcgcgcctcc gttctggttc gtcgcgcgcg
     1621 tcacggtgac ttgcaccgcg ggagcatcgt agacgcctgg tgcaatcgtc gtcgcttcca
     1681 taccggccgg actacccgtc agattccagt agccggcctg aaccgcaccc gtcgtcagcg
     1741 tttgtccgtc cgagcggttc aacgtcacag cgctggtcgc cgctgcctgg gcctgggtcc
     1801 agttcgatcc gttaccgccg gcaaccacca gcgcggaggc gccggcgagt gccgcgctgt
     1861 ccgcgtcgtt ttgcagttcg ttgagtacca cgctcacacg ggcgaggttg atcgcgaatg
     1921 caccgaagca cagcaacgct atgaggaaca acaggaagaa tagtgcgacg gacccgcgtt
     1981 gccggggtgc ggcgtgagga gcccgatttc gcggagggaa agtccgattg cgcatcgccg
     2041 gcctcattcg taattcatta cggtgacggc cgtcaccgtg atcgggccgg tcagcgagct
     2101 gaatgcagaa cccagcacga ggccttgata ctgatagctt acggtgatcg tcagcggact
     2161 gccaggcgac gtacccgcag attgattcac ggttacggtc ggtgtcgtcg cggtgcctcc
     2221 cgtgacaaga ctgttctgca agtaattgag tgcgacattg ctaatctggg cgggcgtcaa
     2281 ctgcggaacc cgcaccacca cgccagcgcg cgccgcttcg cgaccggcat tggtgatgag
     2341 cgccttgtcg catagcagga ggctcacgtc gatgatgccg aacaacacaa tcaacaggaa
     2401 cgggaacacg agaacgaact cgagcgcgat cgctccacgg ctggcgcggg gcagtcctgc
     2461 actgcgcgcc tgcgcacctc gccgcgatcg attcaccatg aacgcgcgcc gattcattgc
     2521 cgtgaggctt tggtgccggg cgaaactgcc ggcgtcgccg tgccgtccgc acgggcggcg
     2581 gcggctcgat actcgtcgag actggagcgc aggctctgat agaagcgcaa gttatcgtcg
     2641 actgacgaag gaggcaagtc gaccagcaga atcttcttcg ccgcatccgc attgccgagc
     2701 aacccgtagg cgagcgccag atcctggcgc gcttgcgatg gcgcatcggc caggccggca
     2761 acgtcgagca ggacgttcgt accttcgcgc ggcttgtctt gaaggatcag cgaaaggccg
     2821 aggttcacgc gcaagccttc tacctcggga tgcaaggcga gcgccttgcg ataaacgtcc
     2881 tgcgcttcac tgtggcgctc ctgcaggtcc agcacggtgc cgagaccctc gctgatcact
     2941 ggattgtcag gctgcgccgc gagcaggccg cgatagagcg ccgctgcatc atcgaggcga
     3001 cgctgccgta acgcgatgcg cgcgagacca agctgcgctg ccggtcgtgc tggaagctga
     3061 agagccgcct gttggtatag cgcgcgggcg cgctccaggt cgccggtctc gtatattgag
     3121 tcggcgagac ccagttgcgc atcgagtgag ttcgggttgg acttgagcgc tttctcgaac
     3181 agcgtggtgg cgagttcgac gtcgcccgcc tgcaacacac tgtctgcgag ccgttcatcg
     3241 ctcgtgccgt cggtgccatg ttgcaacgat actgggcggg ggttgaaact gccgccgctg
     3301 gcacagccag ccaacaggcc gcaggccagc ggcaacgtag tcgtgagtgt ccgcaacagc
     3361 acgggaaaat gtttcatttg aatatgctca ccaggcgaat caccgccggt ccggctgcta
     3421 tcaggccgac cgtcggcaga atgaatagca tcatcggcaa cgtcatcttg accgacagtt
     3481 tcgcggcctt ttcttcgagc gctagcatct gcgcggtccg ctcggctcga gacagcatgc
     3541 gaagcgattg cgtgatcggc gtgccgtatt gttgcgactg aaccagcgtg gttacgagcg
     3601 cgcgtactga cggcaggcca attcgggcag ccaggcggcg cagtgcgacc gcgctgtcac
     3661 cgccaatctg caactcgtcg ccggtgacag agaactcgtc ggcgagcggc ggacaaatcg
     3721 ttttcaattc gcgcgcaacg cgcttcacgc ctactaccag actgttgccg gcattggtgc
     3781 aaatcaccag cagatcgagc gcatcgggca ggcatgacgc tattgctttt tggcgacgac
     3841 tgaccatggc gccgagcgcc aactcgggca ggatcatccc gacaatgaac gccccaccca
     3901 tcatcagtgc gcgaaacacg aagtagccgc cgattcgagg cacatgagga ccgagcacga
     3961 tcgccgccag tgccggcaat gcgccggcca gcagcttgag gccgatcatc acggagaccg
     4021 cccggcgctc acgaaacccc gcgcggacca gttgctcggc caacttgttg cgctgagccg
     4081 catcgcccag cggaacgctt tcgccaagcg tcgcgaaacg ccttatgagc cgttgccaca
     4141 tctccgtagc gtgctcttcc gcgcccggca tgtgctgtgt gtccgcgccc atcgccgcgt
     4201 ggtacgcccg ctgtgcgatg cggcggcgcg ttctacgcgg ccacatcagc acgaacagca
     4261 ggccgccgca cagcacgagc agcagcgcca gttcgcgagc cgtatcggta gcaatgaaat
     4321 tcatcgtgat gtgtccagct tcgaaatttt ttgaatcatc gagaggccgg ccacgagaag
     4381 cactaccgcc aacgttagca tcttgtggcc ggcgcgcgta tggaacagca gctccacata
     4441 gggccggttg atcacataga gcaggccggt gatcaagaac ggcaccgccg caatgatctt
     4501 gcttgcgatg cgaccttctg cggtcagcgc gctggttttc agtcgaatgt ccctgcggga
     4561 gcgaataatg cccgacaggt tctcaagggt ttctccgaga ttcccgcccg tttcgcgctg
     4621 caacagcagg catactgcaa aaaaagaaaa atcggcgatc tgcagtcgtt cgacggcctt
     4681 gtcgagcacc tctttcaggt ctgcgccaag ttgcaagctg tcgcccatcg tgcgaaaggt
     4741 cgagcgcact ggttcctccg cctcgtgccc agccgtgctg attgcctgca ctaccggtat
     4801 tcctgcgcgc acggcccgga tgatcaggtc cagcgtatcc ggaaacaccg tgagaaaccg
     4861 cgtgcgaaag cgcacgatca aaaatcggtg ggtcatcccc atcgccagga ccggcgtgcc
     4921 tccgtagacg aacggaccgg accatcccgg aagcggtgaa aggcgcacga tgataaacgc
     4981 tatcgaagcg ccgacgatgc cactggcgaa tacgaggcgg atgccgcccg caccgctcac
     5041 ggctcgcaag cgctgcacgc gttgctggaa ccacgcttcg atcgcgccat agtcctttgg
     5101 cagctcgaag agttctccac ttgtatcccg cgtcgcgggt tgctgacttg cgacgcgcat
     5161 ctgctgaatc tggctcagcc ggtcctggat gcgaggcttt ggccggcgac gagccatatc
     5221 gcgcagcgac gacacgatca ttccggccag tacgatggcg aaaaacgcac ccgccgccac
     5281 gatgtccgct ggagtcatgg gcgcatcgcc tccatgagcg tcgtttcaag gccatagtaa
     5341 gcggcgcgtg tcgcgaacac cggacgcaac gacgacgact cgaacgtgcc tttgacgtct
     5401 tcctgatagg cgctggcgtc gtagcggaag gcgaaaagat cctgcgtgat gatcacgtcg
     5461 ccttccattc cagcaagctc ggtcacgcgc gtgacgcgcc gcacgccgtc gcgcatgcgt
     5521 tcgatttgaa gaatcaggtg cacggcgctg gcgatctgcc gtcggatcgc cgtgagcggc
     5581 agattgccgt ttgccatcat caccatgctt tcgagtcgcg tgatggcgtc gcgcggcgtg
     5641 ttggcgtgta ttgtcgtcat cgagccgtcg tggcccgtgt tcatcgcctg cagtacgtcg
     5701 aatgcttcgg ggccacgcgt ttcaccgagg atgatgcggt ctggccgcat tcgcagtgcg
     5761 ttacgcacca agtcgcgctg ggataccgcg ccgagtcctt cactgttctc gggacgcgtt
     5821 tcaaggctga ccacgtgcgg ctgttgcagt tgcagttccg cggcgtcctc gatggtcacg
     5881 atacgttcgt gttcgtcgat gtggtgcgac agtgcgttga gcaaggtggt cttgcccgag
     5941 cccgtgccgc ccgagatgat gatgttgagg cggcaggcgc tggcgatctt gagcacccgc
     6001 accatctcgg gcgagatatt ggcttgctgg gccatgcgcg caagcgtgat attgcgcttg
     6061 gaaaacttcc ggatcgaaat cgacgcgcct cgcagcgcaa tcggcggcaa caccacgtta
     6121 acgcggctac cgtcggcaag tcgagcgtcg accatcgggc tgctctcgtc cacgcgtcgg
     6181 ccgaccgcag cggcgatacg ctgtgccacg ttgaccacat gtgcgttgtc gcggaatttc
     6241 agcggcgtga gttcgagtcg gccgtgccgc tccgcgtaga cctgatccgg gccgttgacc
     6301 agtatgtccg tcacggtctc gtcagccaga agcggttcga tcggcccgac accgaacatg
     6361 tcattgagta tttcgctaac gatcagtgcc tgctccgcag cagtgacatt cagttgctct
     6421 cgcgtcgcta cgtcgacggc gatctcctca atccccgtgc gcacctgatc gcgtgtcttc
     6481 atcagcgccg cggacatgtt catcgaggca aacaccacgg cacggatctt cttgaagttg
     6541 tccgagcgaa tcagcgcctc gtgaccgtct gtcggcatgc gcggatgcgc gggcggagcg
     6601 gccgccggtt gctcggcgcg cacgtgtgcg tcggcgagca acgaatccgt cggcatacgc
     6661 ttttttgcgc caaacattac ggcgttctcc tcggtgtcat cagacgggca tgccacggaa
     6721 gaggcgccgc tgacgattcg cggccggtca ggccgttcgc gatccgttcg atagctgcgg
     6781 cgaacccggg ctgctttttg cgagcgacga tcggctcgcc cagattttcg gccctcgcga
     6841 gggacttcgg atcgtacggc agctcgtgca acaccgagcg cccaagcgca cgctggaagt
     6901 ccttgggctg aacgcggtcc gcgaccggtt caagcggatt gttcagcagc accgagacga
     6961 ccggttctcc ggtacgatct tcggcgaacc ggagcagccg cacgcattca cgcgccgcat
     7021 gtaccgaagg atcggcaacg atgcagacaa cctgggacgc atcgagggct tc
//
LOCUS       DATASH010000065         5924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1195128,
            whole genome shotgun sequence.
ACCESSION   DATASH010000065 DATASH010000000
VERSION     DATASH010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5924
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195128"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1650
                     /locus_tag="VHV99_01925"
     CDS             <1..1650
                     /locus_tag="VHV99_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3400 domain-containing protein"
                     /protein_id="HEX3634246.1"
                     /translation="GADLRNAYTPSFGLMGYESLIMQQSDIGAISESIKDCLRCGKCK
                     PVCATHVPRANLLYSPRNKILATSLLVEAFLYEEQTRRGVSIKHWDEFNDVADHCTVC
                     HKCVTPCPVKIDFGDVTMNMRNLLRKMGKKKFNPGNAAGMFFLNATNPQTINLARTAM
                     MGIGYKAQRLGNDVLKKFAKKQTAHPPATVGKPKVTQQVIHFMNKKMPGNLPKKTARA
                     LLDIEDNKIVPIIRNPKTTTADTEAVFYFPGCGSERLFSQVGLATQAMLWEAGVQTVL
                     PPGYLCCGYPQRGSGQFDKAEQIVTDNRVLFHRVANTLNYLDIKTVVVSCGTCYDQLA
                     GYEFEKIFPGCRIIDIHEFLLEKGIKLEGVNGVRYMYHDPCHSPIKTMDPVKLVNQLM
                     GSENDGYKIEKNDRCCGESGTLAVTRPDISTQVRFRKEEEMRKGAAKLRALPPGGAPL
                     VAEAGANAINTANASAGAAGAPNGSVLKAGDGPQPNGATDVKILTSCPSCLQGLSRYN
                     EDAGTEADYIVVEMARHVLGEDWMADYVQRANNGGIERVLV"
     gene            1685..2113
                     /locus_tag="VHV99_01930"
     CDS             1685..2113
                     /locus_tag="VHV99_01930"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEX3634247.1"
                     /translation="MECVFCRDDGGDVLWQDDTLRVVLADEHDYPGFCRVIWNKHVAE
                     FSDLAGSDRDRMMKAVYAVEHAMRRIMQPVKVNLASLGNQVPHVHWHVIPRYSNDAHF
                     PLPIWAPRQRTVSEAMLSARRAQATLLREAVRQEIERALG"
     gene            2199..2612
                     /locus_tag="VHV99_01935"
     CDS             2199..2612
                     /locus_tag="VHV99_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEX3634248.1"
                     /translation="MSGLTPDTPVPTGVVVHSKSRVLELQYANGESYRLPFELLRVYS
                     PSAEVMGHGPGQETLQTGKRDVTITMIEGVGNYALQPTFSDGHATGIYSWDLLHDMAV
                     RQDELWRDYLAKLAAAGVDRDAPMAVAGAAHGHCH"
     gene            2680..3411
                     /gene="ubiE"
                     /locus_tag="VHV99_01940"
     CDS             2680..3411
                     /gene="ubiE"
                     /locus_tag="VHV99_01940"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011546189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEX3634249.1"
                     /translation="MSKTHFGFQSVDEQEKAQKVAGVFHSVASNYDLMNDLMSGGLHR
                     AWKMFTIAQANVRPGFKVLDIAGGTGDLSKAFAKQAGETGEVWHTDINESMLRVGRDR
                     LLDKGVITPALLCDAEKIPFPDNYFDVVTVAFGLRNMTHKDVALAEMRRVLKPAGRLL
                     VLEFSKVWDPLKKVYDVYSFKVLPWLGDRFAKDADSYRYLAESIRMHPDQETLKTMME
                     QAGLDGVKYYNLSAGVVALHVGTKY"
     gene            3450..4442
                     /locus_tag="VHV99_01945"
     CDS             3450..4442
                     /locus_tag="VHV99_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HEX3634250.1"
                     /translation="MSDSGVLSPRKVKRSLVRRIGLIAMVGLIMAGSLASLDAEARRM
                     GGGRSLGRQSNTVQQQSAPSQPSQSNQAMQQRAQPAPAPAPTPAAAPNRSRWLGPIAG
                     LAAGLGIAALLSHFGLGGAFAGAMANIIVIAIIAMIGIWLIRRFMGRKRDTSQPAYAG
                     SSPSLNAGNTGYSQEPRYSAPPTGAYLEPQGNPLATPSVNAVPAVPAGFDSEAFLRNA
                     KVYFVRLQAAWDVGNTEDIREFTTPEMFAEVKVDLSSRGAESNQTDVVQLNAELLGVE
                     ERATEYFASVRFSGLIRESAGAAAEPFVEVWNLTKSNRPGEGWLLAGIQQVAQH"
     gene            4596..5240
                     /locus_tag="VHV99_01950"
     CDS             4596..5240
                     /locus_tag="VHV99_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEX3634251.1"
                     /translation="MTLAAKPFAAAVNHLLARESWARERLAPYAGKTASLSCPPVVLM
                     LLVQPDGYLSAVEATEAQQFDVTISVPSDALPAFVQGGQAAVMKHVKIDGDAEFATVI
                     ARLAEHLRWEPEEDLARLIGDGPAWRVASVVRTVGEHARRTGRNLLDTAAEYLLDENP
                     QLVRRTALEDFNVELARARDALARVEKRIERLEQKVEARGANAPGGAATSRGTR"
     gene            5256..>5924
                     /locus_tag="VHV99_01955"
     CDS             5256..>5924
                     /locus_tag="VHV99_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEX3634252.1"
                     /translation="MRFLRFLKIFFTVIRFGLDEMMLSRVNDRRVRLLLRITTIGRKF
                     DAPPGVRLRLALESLGPIFVKFGQVLSTRRDLLPVDIANELAKLQDQVPPFDSAVAIG
                     LVEKSLGAPVDVLFDDFERVPVASASIAQVHFARVKAGQHAGKAVAVKVLRPNMLPVI
                     DSDLALLRDIAVWAERLWADGKRLKPREVVAEFDKYLHDELDLMREAANGSQLRRNFA
                     GLDLL"
BASE COUNT         1124 a         1815 c         1919 g         1066 t
ORIGIN      
        1 ggcgccgacc tgcgcaacgc ctacacgccg agcttcggcc tgatgggcta cgaatcgctg
       61 atcatgcagc agtccgacat cggcgcgatt tcggaatcga tcaaggactg cctgcgttgc
      121 ggcaagtgca agccggtgtg cgcgacccat gtgccgcgcg ccaacctgct gtacagcccg
      181 cgcaacaaga ttctcgccac ttcgctgctg gtcgaggcgt tcctgtacga agagcagacc
      241 cgccgcggtg tgtcgatcaa gcactgggac gagttcaacg acgtcgccga tcactgcacc
      301 gtctgtcaca aatgcgtgac gccgtgcccg gtgaagatcg acttcggcga cgtgacgatg
      361 aacatgcgca acctgctgcg caagatgggc aagaagaagt tcaatccggg caacgcggcc
      421 ggcatgttct tcctgaacgc caccaatccg cagaccatca atctcgcgcg caccgcgatg
      481 atgggcatcg gctataaagc ccagcgcctc ggcaacgacg tgctgaagaa gttcgcgaag
      541 aagcagacgg cgcacccgcc ggccacggtc ggcaagccga aggtcacgca gcaggtgatc
      601 cacttcatga acaagaagat gccgggcaac ctgccgaaga aaaccgcgcg tgcgctgctg
      661 gacatcgagg acaacaagat cgtcccgatc atccgcaatc cgaagacgac caccgccgat
      721 acggaagcgg tcttctactt cccgggctgc ggctcggagc ggctgttctc gcaagtcggt
      781 ctggcgacgc aagccatgct gtgggaggcg ggcgtgcaaa ccgtgttgcc gccgggctat
      841 ctgtgctgcg gctatccgca gcgcggctcg ggccagttcg acaaggccga gcagatcgtc
      901 acggataacc gcgtgctgtt ccaccgcgtc gccaatacgc tgaactatct cgacatcaag
      961 acggtggtgg tgtcgtgcgg cacgtgttac gaccagctcg ccggttacga attcgagaag
     1021 atcttcccgg gctgccggat catcgacatc cacgagtttc tgctggaaaa aggcatcaag
     1081 ctcgaaggcg tgaacggcgt gcgttacatg taccacgacc cgtgccattc gccgatcaag
     1141 acgatggatc cggttaagct cgtcaatcag ctgatgggtt cggagaacga cggctacaag
     1201 atcgagaaga acgaccgttg ctgtggtgaa tccggcacgc tcgcggtgac gcgcccggat
     1261 atttcgacgc aggtccgctt ccgcaaggaa gaggaaatgc gcaagggggc cgcgaagctg
     1321 cgcgcgcttc ctccgggcgg cgctcctctg gtggccgaag ctggcgccaa cgcgatcaac
     1381 accgccaatg catcggccgg cgcggcgggc gcgccgaacg gttccgtgct caaggccggc
     1441 gacgggccgc aacccaacgg cgccaccgac gtgaaaatcc tgaccagctg cccgtcctgc
     1501 ctgcaaggct tgtcgcgcta caacgaagac gcgggcaccg aggcggacta catcgtcgtg
     1561 gaaatggcgc gtcacgtgct gggcgaagac tggatggcgg attacgtcca acgggcgaac
     1621 aatggcggaa tcgagcgcgt gctggtttaa tggcaccact gacgattttt gcctgaggac
     1681 gacgatggaa tgcgtttttt gccgtgacga tggcggtgat gtgctgtggc aggacgacac
     1741 cttgcgcgtc gtccttgccg acgaacacga ttacccgggc ttctgccggg tgatctggaa
     1801 caagcatgtg gccgagtttt ccgatctcgc cggaagcgac cgcgatcgca tgatgaaagc
     1861 cgtgtacgcg gtcgaacacg cgatgcggcg catcatgcaa ccggtcaagg tcaatctggc
     1921 gagccttggt aaccaggtgc cgcacgtgca ttggcatgtg atcccgcgtt attcgaacga
     1981 cgcgcatttt cccctgccca tctgggcgcc gcgccagcgc acggtgtccg aggccatgct
     2041 gtcggcgcgc cgcgcgcaag cgacgctgct gcgcgaagcg gtgaggcagg aaatcgaacg
     2101 ggctcttggc tgagcgccgc tgaatctttc gcctggcgcg gggccttcga gcggggcgaa
     2161 aaacccgggg cagcccggcg tgttcttatg aggtcaatat gagtggactg actcccgata
     2221 ccccggttcc gaccggtgtg gtcgtgcatt ccaaatcgcg cgtactcgaa ttgcagtacg
     2281 caaacggcga atcgtatcgc ctgccgttcg aactgctgcg cgtgtactcg ccgtcggcgg
     2341 aagtgatggg ccacgggccc ggccaggaaa ccctgcaaac gggcaagcgt gacgtcacga
     2401 tcacgatgat cgaaggcgtc ggcaactacg cgttgcagcc gactttctcc gacgggcatg
     2461 ccaccggcat ctattcctgg gacctgttgc acgacatggc cgttcgtcag gatgaactct
     2521 ggcgcgacta tctcgccaag cttgcagcgg ccggtgtcga ccgcgacgcg ccgatggccg
     2581 tagcgggcgc tgcgcacggg cactgtcact gatacgattc gccctggtcg ctgcgctgat
     2641 gcggcgcaac atttcagaat acgcgaaagg acgagcgcga tgagtaaaac ccacttcggc
     2701 tttcaatcgg tcgacgagca ggaaaaagcg cagaaggtgg cgggcgtgtt tcactcggtc
     2761 gcctccaact acgacttgat gaacgacctg atgtcgggcg ggttgcaccg ggcgtggaag
     2821 atgttcacga tcgcgcaggc caacgtccgg ccgggtttca aggtgctcga tatcgcgggg
     2881 ggcacgggcg acctgtcgaa ggcatttgcc aaacaggcgg gcgaaaccgg tgaagtctgg
     2941 cacaccgaca tcaacgaatc gatgctgcgg gtgggccgcg accgcctgct ggacaagggc
     3001 gtcattaccc cggcgctgct ctgcgacgcc gagaaaatcc cctttccgga caattacttc
     3061 gacgtggtga ccgtggcatt cggcttgcgc aacatgacgc acaaggatgt cgcgctggcg
     3121 gaaatgcgcc gcgtgctgaa gccggcaggc cgtctgctgg tgctggaatt ctcgaaggtg
     3181 tgggatccgc tcaaaaaggt ctacgacgtc tattctttta aggtattgcc gtggcttggc
     3241 gaccgttttg cgaaagacgc cgatagctac cgttacctgg cggaatcgat ccggatgcat
     3301 ccggaccagg aaactttgaa aacaatgatg gaacaagcgg gcctggacgg cgtcaaatat
     3361 tacaatttgt cagctggcgt ggtagcttta catgtgggga ccaaatacta gggttcctaa
     3421 cccatcgatt tttaaaggaa agtacgaaaa tgtccgattc aggtgtgtta tctccccgta
     3481 aggttaagcg gtcgttggtg agaagaatcg gactgatcgc gatggtcggt ctgatcatgg
     3541 ccggctctct cgcctcgctc gatgcggaag ctcgccgcat gggcggtggc cgtagtcttg
     3601 gccgtcagtc gaatacggtt cagcagcaat ccgccccgtc gcaaccttcc caaagcaatc
     3661 aggcgatgca gcagcgcgcc cagccggctc cggcgcccgc gccgactccg gccgccgcgc
     3721 ctaaccgctc gcgctggctt ggcccgatcg ccggtctcgc ggccggcctg ggtatcgctg
     3781 cgttgttgtc gcacttcggg ctgggcggcg cgtttgccgg cgccatggcc aacatcatcg
     3841 tgattgcgat catcgccatg atcggcatct ggttgatccg ccgcttcatg ggccgcaagc
     3901 gcgacacgtc gcaaccggcg tacgcgggca gctcgccgtc gctgaatgcg ggcaacaccg
     3961 gctattcgca ggaaccgcgc tacagcgcgc cgcccaccgg cgcgtatctc gaaccgcaag
     4021 gcaacccgct ggccacgccg tccgtcaatg ctgttccggc agtgccggcc ggcttcgatt
     4081 cggaagcgtt cctgcgcaac gccaaggtgt acttcgtgcg gctgcaagct gcatgggacg
     4141 tcggcaacac ggaggacatc cgcgaattca ccacgccgga aatgttcgcc gaagtgaagg
     4201 tcgatctgag ctcgcgcggt gccgagtcca atcagaccga cgtggtgcag ttgaacgcgg
     4261 agttgctggg cgttgaagag cgcgcaaccg aatacttcgc cagcgtgcgc ttctccggct
     4321 tgatccgcga atcggcgggt gcggcggctg agcctttcgt cgaagtctgg aacctgacca
     4381 agtcgaaccg gccgggcgaa ggctggctgc tggccggtat tcagcaggtg gcgcagcact
     4441 gaagcagtag cagggcggag acagggcagt cctgcccgcc ttgattctgc gcacaggatt
     4501 ggccgttcgg catagcaagc cgcgaagcga acggacgtta caataggaac ccgcgcgggc
     4561 acctcgcctg cgcgggtttt ttcttgccac tttcaatgac cctcgccgcc aagcccttcg
     4621 ctgctgctgt caatcatctg ctcgcccgcg aatcgtgggc tcgcgagcgt ctcgcccctt
     4681 acgcgggcaa gaccgccagc ctgtcttgtc cgcccgtcgt gctgatgctg ctggtgcagc
     4741 cggacggcta cctgagcgcg gtcgaggcaa ccgaggcgca acagttcgat gtgaccatct
     4801 cagtgccgtc cgacgcgctg ccggctttcg tgcaaggcgg tcaggccgcc gtgatgaagc
     4861 acgtcaagat cgacggtgac gcggagttcg cgacggtgat cgccaggctc gccgagcatt
     4921 tgcgctggga accggaagag gatctggcca ggctgatcgg cgacggtccg gcgtggcggg
     4981 tcgcgtcggt cgtgcgcacg gttggcgaac acgcgcggcg caccgggcgc aatctgctcg
     5041 acaccgccgc cgaatatctg ctcgacgaaa atccgcaact ggtgcggcgc accgcgctgg
     5101 aggatttcaa cgtcgaactg gcccgcgccc gcgacgccct ggcgcgcgtg gagaagcgca
     5161 tcgagcgtct tgaacagaag gtcgaagccc gcggcgccaa tgcgccgggc ggcgccgcca
     5221 cgtcgcgcgg cacgcgctag tcaccgggca caactatgcg ttttctgcgt ttcctcaaga
     5281 ttttcttcac ggtcatccgc ttcggtctcg atgagatgat gctcagccgc gtcaacgacc
     5341 ggcgtgtgcg tctgctgctg cgtatcacca cgatcggccg caagttcgac gcgccaccgg
     5401 gcgtgcgctt gcggctcgcg ctcgaaagtc tcgggccgat cttcgtcaag ttcggccagg
     5461 tgctgtccac gcggcgcgat ctgctgccgg tggacatcgc caatgaactg gccaagctgc
     5521 aggatcaggt gccgccgttc gactcggcgg tggcgatcgg attggtcgag aagtcgctcg
     5581 gggcgccggt cgacgtgctg ttcgacgatt tcgagcgggt gccggtcgca agcgcgtcga
     5641 tcgcgcaggt tcacttcgcg agggtgaaag cagggcagca cgcgggtaaa gcggtcgctg
     5701 tgaaagtgct gcggccgaac atgctgccgg tgatcgactc cgacctggcc ttgctgcgcg
     5761 acatcgccgt ctgggccgag cggctgtggg ccgacggcaa gcggttgaag ccgcgcgagg
     5821 tggtcgccga attcgacaag tatctgcacg acgaactcga tctgatgcgc gaagcggcca
     5881 acggcagcca gctgcgccgc aacttcgccg ggctcgatct gctg
//
LOCUS       DATASH010000066         5714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3766640,
            whole genome shotgun sequence.
ACCESSION   DATASH010000066 DATASH010000000
VERSION     DATASH010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5714
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3766640"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..1631
                     /locus_tag="VHV99_01960"
     CDS             129..1631
                     /locus_tag="VHV99_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX3634253.1"
                     /translation="MTHSIHGNKRWLALIVLCLGVLMIVLDTTIVNVALPSIAADLGF
                     TETSLVWVVNAYMLTFGGCLLLGGRLGDLYGHRKLFLGGITLFTLASLACGMADSQIL
                     LVCARAVQGLGGAVVSAVSLSLIMNLFTETGERAKAMGVYGFVCAGGGSIGVLLGGLL
                     TNLLSWHWIFLVNLPIGIAVYGLCVALLPSARGHAHGERLDVAGAVTVTVSLMLAVYA
                     VVNGNEAGWTSPQTLGMLFAAVVLLAMFLVIEARVQHPLMPLGLFRLRNVATANVVGV
                     LWAAAMFAWFFISALYLQRVLGYRPMQVGLAFLPANLIMAFFSLGLSARVVMRFGLRV
                     PLAAGLLVAACGLALFARAPVNGSFVLDVLPGMLLLGFGAGIAFNPMLLAAMSDVDPS
                     DSGLASGIVNTSFMMGGALGLAVLASLAAARSGAMQASENAVAALNSGYHVAFLFGAI
                     FAAAAGIVGGLLLRPGQPGGADAHADAQGHSTSASGHSRSAGNEAATENY"
     gene            1792..2625
                     /locus_tag="VHV99_01965"
     CDS             1792..2625
                     /locus_tag="VHV99_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX3634254.1"
                     /translation="MNPVGKALWFIESHFAQALTLDEIANRGCVSRFHLARAFEAATG
                     LSVMRYVRARRLSEAARRLADGAPDILTVAIDAGYGSHEAFTRAFREQFGLTPEALRA
                     RGHLDSIAIVEPIKMDETLLTHLEPPRFEDGQPFLVAGLSERYTCDTSTAIPSQWQRF
                     GAYYGKVPGQMGGVAYGVCYNADDAGNFDYLCGVEVSDFSALPAELSRVRIAAQRYAV
                     FSHREHISTIRRTLNTIWNQWLPASGHVPADAPNFERYSEQFDPVSGMGGLEIWLPLK
                     T"
     gene            complement(3062..4489)
                     /locus_tag="VHV99_01970"
     CDS             complement(3062..4489)
                     /locus_tag="VHV99_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013689534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX3634255.1"
                     /translation="MKQPDELLHTVYVKADRLMIAVVWGLFAISCLLAIRDYTMRAVL
                     WVGLPTVAVASLLAYWRAGALVTRLFLAASLMTFAALQIHQERGLTELHFGIFVLMSF
                     LLAYRDWRPILCAACVIAVHHFTFNYLQLAGLDVYCFTEPAWSTVLIHAAYVVVQAGL
                     LIFIARHMKADAQTGRELAVLGENLSRQAGQFDLRLPPMKLEGASSRTFKDTLNAIHN
                     AMREISATIDQMAVSSDNIAAENHTLSQEFTTQAETLNATNTAMEQIAQRVRESAEHA
                     ASANSLARQTSTVVQQSEQVVSEVVDKMTEIDQAVHRMGDMIAMIESIAFQTNLLALN
                     ASVEAARAGSHGRGFSVVAEEVRTLAHRSASAAREIKGLIGDSLQRVEHGSTLATRAG
                     VAMRRVVGDVADVARLIEHISASSDAQSHDVDRFSQGMGKMDAMLERDVKHVKGVASA
                     SASLREKARTLREATSIFLVEHDVN"
     gene            4977..5447
                     /locus_tag="VHV99_01975"
     CDS             4977..5447
                     /locus_tag="VHV99_01975"
                     /inference="COORDINATES: protein motif:HMM:NF019157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HEX3634256.1"
                     /translation="MQATFNVDTAYPVIAAGLQSAGADHSLPAIAQYLVSGKKLISFH
                     GGADPLLSSRDHLRNWLAMTQLAGSAASNARFYREAGVGHVTGGNGPDQFDYIGPMIA
                     WVEQGVAPGQLTMTKLDTNGNVTATLPDCPYPTVPHYNGSGNINQAASYSCASS"
     gene            complement(5444..>5714)
                     /locus_tag="VHV99_01980"
     CDS             complement(5444..>5714)
                     /locus_tag="VHV99_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634257.1"
                     /translation="RQLRPSAVSCRFQSTIRFEFLIRILPAPHGIIDSVQGRLGNATQ
                     RTEHVTKANQGIGLQIALSPMKTTHALDERQLVAVSRPRHGSVLF"
BASE COUNT         1012 a         1748 c         1749 g         1205 t
ORIGIN      
        1 cggcacgtcg ggcatccgct tgtaccctgc ttgtacccgc ttgtaccctg cttgtacccg
       61 cttgtacccc gcttgtaccc cgcttgtacc cgcttgcatc cgcttgaccg cggcaaagga
      121 gttcccgcat gacccattcc atccacggca acaaacgctg gctggcgctg atcgttctgt
      181 gcctcggcgt gctcatgatc gtgctcgata ccacgatcgt gaacgttgcg ttgccgtcga
      241 tcgccgcgga cctcggcttt accgaaacct cgctggtgtg ggtggtcaat gcgtacatgc
      301 ttaccttcgg cggctgtttg ctgttgggcg gccggctcgg cgatctctat ggtcatcgca
      361 agctgttcct tggcggcatc acgctgttca cgctggcgtc gctcgcgtgc ggcatggcgg
      421 attcgcaaat cctgctggtg tgcgcgcgtg ccgtgcaggg tttaggcggc gcggtcgtct
      481 ccgcggtgtc gctgtcgctg atcatgaatc tgttcaccga gaccggcgaa cgggccaaag
      541 cgatgggcgt gtacggcttt gtctgcgcgg gtggcggcag catcggggta ttgctcggcg
      601 gtctgctgac caacctgctg agctggcact ggatctttct cgtcaacctg cctatcggta
      661 tcgccgtcta cgggctgtgc gtcgcgctgt tgccgtcggc tcgcggtcat gctcatggcg
      721 aacggctcga cgtggccggc gcggtaacgg tgaccgtttc gctaatgctc gctgtgtacg
      781 cggtcgtgaa cggcaacgaa gccggctgga cctcgccgca gacgttgggc atgctgttcg
      841 ccgcagttgt gctgttggcc atgtttctcg tcatcgaagc gcgcgtgcag catccgttga
      901 tgccgctcgg tctgttccgt ttgcgcaatg ttgcgaccgc caatgtggtg ggcgtgctat
      961 gggcggcagc gatgttcgcg tggtttttta tttccgcgct gtatttgcag cgcgtgctcg
     1021 gttatcggcc catgcaggtc gggctcgcgt tcttgccggc caatctgatc atggcgttct
     1081 tttcgttggg cctgtcggcg cgcgtggtga tgcgctttgg tctgcgcgtt ccgcttgctg
     1141 ccgggttgct ggtcgccgcg tgcggccttg cgctgttcgc gcgcgcgccg gtcaatggca
     1201 gcttcgtgct cgacgtgctg cctggcatgc tcctgctcgg cttcggcgcg ggcatcgcgt
     1261 tcaatcccat gctgctggct gccatgagcg atgtcgatcc gagcgattcc ggcctcgcgt
     1321 cagggatcgt caacacgtcg ttcatgatgg gtggcgcgct gggtctcgcg gtgctcgcga
     1381 gtctcgctgc cgcgcgcagc ggcgcgatgc aggcctccga aaacgcggtg gccgcgctca
     1441 atagcggcta tcacgtggcg ttcctgttcg gtgcgatctt cgcggcggcg gcaggcattg
     1501 tgggcggctt gctgttgcgg ccggggcagc cgggcggtgc cgacgctcat gctgacgcac
     1561 agggtcacag cacatcggca tcgggacatt ctcgctccgc cggcaacgaa gctgcgacgg
     1621 aaaactattg acccaccggg tgagtccggc tcgccggacg acgagtttca cccaccggaa
     1681 cgcacaccgc tcgccccaca ccgcacgcga accgttgcaa aaccgcaagc gcgatcaaat
     1741 acgccggagc gcgcctggct atgctggctt catgcagaca tgagagccga tatgaacccg
     1801 gttggaaaag cgctctggtt tatcgaaagc cattttgccc aggcgttgac gctcgacgag
     1861 atcgccaacc gcggatgcgt gtcgcgcttt catctggcgc gtgcgttcga agcggccacc
     1921 gggctttcgg taatgcgcta tgttcgtgcg cgccgcctga gcgaagcggc acgccgcctc
     1981 gccgacggcg cacctgacat cctgacggtc gccatcgacg ccggttacgg ttctcacgaa
     2041 gcattcacgc gtgcctttcg cgagcaattc gggctcacac ctgaagcgct gcgcgcgcgt
     2101 ggtcatctcg acagcattgc catcgtggag ccgatcaaaa tggacgaaac ccttctcacg
     2161 catctcgaac cgccgcgttt cgaagacggc cagccgttcc tcgtcgcggg cctgagcgaa
     2221 cgctatacct gcgacaccag caccgccatt ccgtcgcaat ggcagcgctt tggcgcttac
     2281 tacggcaagg tgccgggaca gatgggcggc gtggcctacg gcgtttgcta caacgccgac
     2341 gacgcaggca atttcgacta cctgtgcggc gttgaagtca gcgacttttc ggcgctgccc
     2401 gcggagttga gccgcgtccg cattgccgcg cagcggtatg ccgtattcag ccatcgcgag
     2461 catatctcga cgatccggcg caccctgaac acgatctgga atcaatggct gccggcgtcg
     2521 ggacacgtgc ccgcggatgc gccgaatttc gaacgctaca gtgagcaatt cgatccggtc
     2581 agcggcatgg gcggcctcga aatctggttg ccgttgaaga cctgatcgac gcatggcggc
     2641 agtagcggaa tgtgatcaga gcgcgccctg tttcggcctt cgtagcggtt acacggaatg
     2701 cctggtgagt ttcgcagcac cgcgatctgc cgtgaaagac ggccgcatca cactacatgc
     2761 gcgctacgcg ctagaaccga taaccaatgc cgccgagaat cacgtccgtg tcgcggctat
     2821 agcgtgtgac cacgcggttc cacgtcacga gacgagcggt ttatttctcc agtttaccgc
     2881 gacgatgtct ctcaagctca ggggcgattg gtcgcagacc tatcttatgc gactggaaca
     2941 ggctagctag atagcgacct ggaactcgac cctcggcgat agaggtccgc cagaccgctg
     3001 cctcccggta cttcgcgaac ttcgatgtct gtccgatccc cggattgaag cagttgcacg
     3061 atcaattcac atcgtgctcg acgaggaaaa tcgacgtcgc ctcacgcaac gttcgcgctt
     3121 tctcccgcaa gcttgccgac gctgaagcga cgcccttcac gtgcttgaca tcgcgctcca
     3181 gcattgcgtc catcttgccc atgccctgac tgaaccgatc gacatcgtgg ctctgggcat
     3241 cactcgatgc actgatatgc tcgatcaacc tcgcgacgtc cgccacatcg ccgacaacgc
     3301 gccgcatggc tacgccggcg cgtgtcgcaa gcgtcgagcc gtgctcgacg cgctgcaacg
     3361 aatcgccgat cagccctttg atttcgcgcg cggcgctcgc gctgcgatga gcaagcgtac
     3421 ggacctcctc ggcgaccacc gaaaatcccc ttccgtggct acccgcgcgg gccgcctcga
     3481 ccgacgcgtt gagcgcgagg aggttggtct ggaacgcgat gctctcgatc atcgcgatca
     3541 tgtcgcccat ccgatgcacg gcctgatcga tctcggtcat cttgtcgacg acctcgctga
     3601 caacctgttc gctttgctgc accacggttg aggtttgccg cgcgaggctg tttgccgatg
     3661 ccgcatgttc cgcgctttcc ctgacccgtt gcgcgatctg ctccatcgcg gtgttggtcg
     3721 cgttgagcgt ttcggcttgt gtcgtgaatt cctgcgacaa ggtgtggttc tcggcagcga
     3781 tattgtccga tgacaccgcc atctggtcga tcgtcgcgga gatttcgcgc atcgcattgt
     3841 ggatcgcgtt gagtgtgtct ttgaaggtgc ggctgctggc gccttcgagt ttcatgggcg
     3901 gcaggcgcag atcgaattga cccgcttgcc gtgaaaggtt ttcaccgaga acggcgagtt
     3961 cccggcccgt ttgcgcgtcg gccttcatat gccgcgcaat gaaaatcagc aacccggcct
     4021 gcacgacgac gtaggccgca tggatcaaca cggtcgacca cgcgggttcg gtgaagcaat
     4081 agacgtccaa tccggcaagc tggagataat tgaacgtgaa gtgatgcacc gcgatgacgc
     4141 aggcggcgca gaggatcggt cgccagtccc gataggccag caggaacgac atcagcacga
     4201 agatgccaaa gtgcaactcg gtcaggccac gctcctgatg aatctgcaac gcagcgaatg
     4261 tcatgaggct ggcagcgagg aagagacgcg tcacaagtgc gcctgcgcgc cagtaagcca
     4321 gcagcgacgc gacggcgacg gtaggcaagc caacccacag tacggcgcgc atggtgtagt
     4381 cgcgtatcgc caataggcaa gagatcgcaa acaaacccca tacaacggca atcatgagcc
     4441 tgtcggcttt cacatagacc gtgtgcagta actcatccgg ctgtttcatg gggtccctca
     4501 gttatcgttc tttattggtc tggtgtaccg gtgcgccacg tctgccttcc caaagcagcg
     4561 tctcataggg tccgcaggac gtgatgagaa ttgctgccga gttcactcgc gtactttggg
     4621 acaacattgc gggcgccccg gattgccgac gcggggcctt gtcttatcca gcatgacgca
     4681 gatgcataac ggcacgcgtg gacgattctt tagacaaata aaagtgctgc agtgccggcc
     4741 agtggagatc agcttctcaa cctccgagga agcattccat tcgacattca ctcggggctt
     4801 gatcgatcga caggtctcgc gccgcatcaa cacgtactgc gctctattcc ggctacgatt
     4861 gggccgactt cggcagttcg ttaggcgtcg gtgcatatac ggtactggga ggcggatttg
     4921 ccggaatcgc gacaggtaat gccaattggc tcctaccggc aatcgcacca ggaagtttgc
     4981 aggcgacctt taacgtcgac accgcctatc cagtcatcgc cgcaggtttg caaagtgccg
     5041 gtgcggatca cagcctgccg gctatcgcgc aatatctggt ttcgggtaaa aagcttattt
     5101 cgttccacgg cggtgctgat ccgctgctgt cgtctcgcga ccatctccgc aactggttgg
     5161 ccatgacgca actcgcggga tcggcggcga gtaatgcgcg gttctaccgc gaggccggtg
     5221 tgggtcatgt caccggcggc aatgggccgg atcagttcga ctacattggc cccatgatcg
     5281 cctgggttga gcaaggcgtc gcacctggtc aactgactat gaccaaactt gacacgaacg
     5341 gcaacgtgac tgcaacgctt cctgactgcc cgtatccgac ggtgccgcac tacaacggct
     5401 caggaaacat caatcaggca gccagctata gctgcgcaag cagttaaaag agcacagacc
     5461 cgtggcgcgg acgagagacg gccacgagtt gtcgctcgtc caacgcatgg gtcgttttca
     5521 tgggtgacag cgcgatctga agaccgattc cctggttagc cttggtgacg tgctctgtgc
     5581 gctgggtggc attgccgagg cgaccttgca ctgagtcgat gattccatgg ggagcgggca
     5641 gtatccgaat caggaactca aaccggatag tggactgaaa tcggcacgat accgctgaag
     5701 gccgaagctg acgc
//
LOCUS       DATASH010000067         1760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2975159,
            whole genome shotgun sequence.
ACCESSION   DATASH010000067 DATASH010000000
VERSION     DATASH010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2975159"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1355
                     /gene="argH"
                     /locus_tag="VHV99_01985"
     CDS             <1..1355
                     /gene="argH"
                     /locus_tag="VHV99_01985"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEX3634258.1"
                     /translation="PMSELVKRYTSSVFFDKRLALVDIEGSLAHASMLAAQKIIAADD
                     LAAIQRGMAQIKGEIERGEFEWQLDLEDVHLNIEARLTALIGDAGKRLHTGRSRNDQV
                     ATDIRLWLRGEIDRIGGLLTELRTALLDMAEKNASTIMPGFTHLQVAQPVTFGHHLMA
                     YVEMFSRDAERMADCRKRVNRLPLGAAALAGTSYPIDRHAVAKTLGFDGICANSLDAV
                     SDRDFAIEFTAASALIMTHVSRFSEELVLWMSPRVGFIDLADRFCTGSSIMPQKKNPD
                     VPELARGKTGRVNGHLIALLTLMKGQPLAYNKDNQEDKEPLFDTVDTVADTLRIFAEM
                     VAGISVKPQAMRDAALQGFSTATDLADYLVKRGLPFRDAHEAVALAVRVCADRGCDLA
                     DLTLEEMRKELPNVAHLIGEDVFSYLTLEGSVASRNHPGGTAPEQVLAAVKAAREALK
                     "
BASE COUNT          349 a          543 c          566 g          302 t
ORIGIN      
        1 agccgatgtc ggagctcgtc aaacgctaca cgtcgtcggt tttcttcgac aagcgtctgg
       61 cgctcgtcga tatcgaaggg tcgctcgcgc acgcctcgat gctggccgcg cagaagatca
      121 tcgccgccga cgacctcgcc gcgatccagc gcggcatggc gcagatcaag ggtgaaatcg
      181 agcgcggcga attcgagtgg caactcgatc tggaagacgt ccacctgaac atcgaagcgc
      241 gcctgaccgc gctgatcggc gacgccggca agcgcctgca cacgggccgc tcgcgtaacg
      301 accaggtggc gacggatatc cgcctgtggc tgcgcggcga aatcgaccgc atcggcggct
      361 tgttgacgga attgcgcacg gcgctcctcg acatggcgga gaagaacgcg tcgaccatca
      421 tgccgggctt cacgcacctg caggtcgccc agccggtcac gttcggtcat cacctgatgg
      481 cttacgtcga aatgttctcg cgcgacgccg aacgcatggc cgattgccgc aagcgtgtga
      541 accggctgcc gctgggcgca gccgctttgg ccggcaccag ctatccgatc gaccgtcatg
      601 cagtggcgaa gacgctcggc ttcgacggca tctgcgcaaa ctcgctggac gcggtgtccg
      661 atcgtgactt cgcgatcgaa ttcacggcag cgtcggcgct catcatgacg cacgtgtcgc
      721 gcttctccga agaactcgtg ctgtggatga gcccgcgcgt cggcttcatc gatctggccg
      781 accgtttctg caccggttcg tcgatcatgc cgcagaagaa gaacccggac gtgcccgaac
      841 tcgcgcgcgg caagaccggc cgcgtgaacg gccatctgat cgcgctgctg acgttgatga
      901 aaggccagcc gctcgcgtac aacaaggaca atcaggaaga taaggagccg ctgttcgaca
      961 ccgtcgatac ggtcgcggac acgctgcgca tcttcgctga aatggtcgcc ggtatttcgg
     1021 tgaagccgca agccatgcgc gacgccgcgt tgcaaggctt ctccacggca accgatctcg
     1081 ccgactacct ggtcaagcgc ggcctgccgt tccgcgacgc gcacgaagcg gtggcgctcg
     1141 cggttcgcgt gtgcgcggat cgcggctgcg atctggcgga cctgacgctc gaggaaatgc
     1201 gtaaggaact gccgaacgtc gcgcacctga tcggcgaaga cgtgttctcg tatctgacgc
     1261 tcgaaggttc ggtggcgagc cgtaaccatc cgggcggcac agcgcccgag caggtgctcg
     1321 cggcggtcaa ggctgcacgg gaagcgttga agtaaggtag ggcctggcgc gcgttagcgc
     1381 gtccggtagc ccccctgtgc gtgcatcgtg tggctcagcc acattacctc gggcgtcact
     1441 gcgggcacta aacgaagcac gcaaacaaag gcgaactcat cgacgggttc gcctttttca
     1501 atttcgcggc tggctggatg gatggatgga tggcgcaaag ggtcgcgcaa aaaagaaagc
     1561 ccgcgcgaag cgggctaaac gaaagacttc caccgatgcc tctcgaaaga ggccaacgaa
     1621 gtgtaggcgt gtttgggcag aagagaaaac agttcatatt tcagcctatt acaaagcacg
     1681 cacgggttcg gtgtgcaagc tgaaacgggc ggatgcaccg cccgtcgctt cagggcacgg
     1741 ccgtctccgg caacgagtga
//
LOCUS       DATASH010000068         2139 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_997829,
            whole genome shotgun sequence.
ACCESSION   DATASH010000068 DATASH010000000
VERSION     DATASH010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_997829"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1729)
                     /gene="pnp"
                     /locus_tag="VHV99_01990"
     CDS             complement(<1..1729)
                     /gene="pnp"
                     /locus_tag="VHV99_01990"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011657486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX3634259.1"
                     /translation="MTMFNKIVKEFQWGQHKVRLETGEIARQASGAVIVDVEDTVVLA
                     TVVGAKTAKPGQDFFPLTVDYLEKTYAAGKIPGGFFRREGRPSEGETLISRLIDRPLR
                     PLFLEGFYNEVQVVIHVLSLNPEIPADIPALIGASAALAVSGLPFNGPVGAARVAYIN
                     NEYVLNPTRPQMKESALDLIVAGTERAVLMVESEAQQLSEEVMLGGVVFGHEQMQIAI
                     DAIHELVREGGKPEWDWQPAAKNEPLIARVTELAQAELLAAYQLRDKQARSTKLKEVY
                     AATSAKLEEDAAAGGTVAADKATVGNVLFDIEAKIVRTQILNGEPRIDGRDTRTVRPI
                     EIRTGVLPRTHGSALFTRGETQALVVATLGTKGDEQNIDALEGEYRERFMLHYNMPPF
                     ATGETGRVGSPKRREIGHGRLAQRALAACLPSADEFGYSIRVVSEITESNGSSSMASV
                     CGGCLALMDAGVPMKAHVAGIAMGLILEGNKFAVLTDILGDEDHLGDMDFKVAGTDQG
                     VTALQMDIKIQGITKEIMQVALAQAKEGRMHILGKMTSAVSGANTVLSDYAPRMITIK
                     INPEKIRDVI"
BASE COUNT          366 a          685 c          641 g          447 t
ORIGIN      
        1 cgatcacgtc gcggatcttt tccgggttga tcttgatggt gatcatgcgc ggtgcgtagt
       61 ccgacagcac cgtgttcgca cccgacaccg ccgaggtcat cttgccgagg atgtgcatac
      121 ggccttcctt cgcttgcgcg agggcgacct gcatgatttc cttggtgatg ccctggatct
      181 tgatgtccat ctgcagcgcg gtcacgcctt ggtccgtgcc cgccaccttg aagtccatgt
      241 cgccgaggtg atcttcgtcg ccgagaatgt cggtcagcac ggcaaacttg ttgccttcca
      301 ggatcaggcc catggcgatg ccggcgacgt gtgccttcat cggcacgccg gcgtccatca
      361 gcgcgaggca gccgccgcac accgaagcca tcgacgagga accgttcgat tccgtgattt
      421 ccgacacgac gcggatcgag tagccgaatt cgtcggcgct cggcaggcat gcagccagcg
      481 cgcgctgggc cagacggccg tgaccgattt cacggcgctt cggcgaaccg acgcggcccg
      541 tttcgccagt cgcgaacgga ggcatgttgt agtgaagcat gaagcgttcg cggtactcgc
      601 cttcgagcgc gtcgatgttc tgctcgtcgc ccttcgtgcc cagcgtggcc accaccagcg
      661 cttgcgtttc gccgcgcgtg aacagtgccg aaccgtgggt acgcggcagc acgccggtac
      721 ggatttcgat cgggcgcacg gtgcgcgtgt cgcggccgtc gatacgcggt tcgccgttca
      781 ggatctgcgt acggacgatc ttcgcttcga tgtcgaacag cacgttgccg acggttgcct
      841 tgtcggccgc caccgtgccg cctgctgcgg cgtcttcttc cagcttcgcc gatgtggctg
      901 cgtagacttc cttcagcttg gtcgaacggg cttgcttgtc gcgcagttgg taagcagcga
      961 gcagttcggc ttgcgccagt tccgtcacgc gcgcgatcag cggctcgttc ttcgccgccg
     1021 gttgccaatc ccattccggc ttgccgcctt cgcggaccag ttcgtggatc gcgtcgatcg
     1081 cgatctgcat ttgctcatgg ccgaacacca cgccgccgag catcacttct tcgctcagtt
     1141 gctgcgcttc cgattccacc atcagcaccg cgcgttccgt accggcgacg atcaggtcga
     1201 gcgccgattc cttcatttgc ggacgcgtcg ggttcaacac gtattcgttg ttgatgtagg
     1261 ccacgcgtgc tgcgccgacc gggccgttga acggcagacc ggacacggcg agcgccgccg
     1321 acgcgccgat cagcgcggga atgtcggcgg gaatttccgg gttcagcgac aggacgtgga
     1381 tcacgacctg gacttcgtta tagaagcctt ccaggaaaag cgggcgcaac ggacggtcga
     1441 tcaggcgcga aatcagcgtt tcgccttccg acggacggcc ttcgcgacgg aagaagccac
     1501 ccgggatctt gccggcggcg taggtctttt cgaggtagtc gacggtcagc gggaaaaagt
     1561 cctgacccgg cttggccgtc ttggcgccga ccacggtggc cagcaccacg gtatcttcga
     1621 cgtcgacaat tacggcaccg ctcgcctggc gagcgatttc gccggtttcg aggcgaacct
     1681 tatgttgtcc ccactgaaat tctttgacga tcttattgaa catagtcatt gcttttcctc
     1741 ttcgtaatgc cgcgcggacc agaagcggcg cggcaacgcc aggaccggcg ccgcatggga
     1801 agagtagtgt tatgccattc cagagagcca cgccctacat cgagtacgcg cgcgaaccgc
     1861 tggaatgaca caaagctccg cccctgaagc ccggccttga atcccttatt tgctgcttgc
     1921 tctactgctt tgttgctttt actacgcctg cctgcccccg ccgcatgaac cggcatgcca
     1981 ctgcggagaa cagccggcgc gcatcacatg aagcgcaccg tgcaaaaaca aaatgcctgt
     2041 atcagtggaa ctgacacagg catcttgctg aacgactggc cgattactta cgcagaccca
     2101 gcttctcgat cagtgcgcgg taacgatccg cgtccttac
//
LOCUS       DATASH010000069         7274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6536455,
            whole genome shotgun sequence.
ACCESSION   DATASH010000069 DATASH010000000
VERSION     DATASH010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7274
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6536455"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="VHV99_01995"
     CDS             complement(<1..685)
                     /locus_tag="VHV99_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006397815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEX3634260.1"
                     /translation="MNLHNHHACRPFLEAGNLSQVSAYYEEGRNIMWMMLRAQPRPCF
                     NLDLVHDILGLAQAARESGLPIDFWVTGSLIPTMFNVGGDLDFFAETIRAGKRQTLMA
                     YARACVDCVHGAARGFDTGAISIAMVEGTALGGGFEAALAHHFLLAQSDARMGFPEIA
                     FNLFPGMGGYSLVARKAGMRLAEELIGVGESHTAEWHFGRGLVDQLFEPGDAYMATRT
                     FIDTLRPKMN"
     gene            complement(1109..2212)
                     /locus_tag="VHV99_02000"
     CDS             complement(1109..2212)
                     /locus_tag="VHV99_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate/lactate/ureidoglycolate dehydrogenase"
                     /protein_id="HEX3634261.1"
                     /translation="MTDNPTAEPTSLRIPADQLHAYVRAIWEQAGSTPREAGLVADHL
                     VAANLTGHDSHGVGMIPRYVASLADAQLQLNLHARVVKDAGAVLTVEGARGFGQVVAF
                     EAMEQGIERAQRFGICAVGLRSAHHIGRIGHWAEQCARAGLVSFHFVNVAGDPLVAPF
                     GGADRRIGTNPFCAAYPRPGKPPLVLDFATSTVAYGKERVAYNQGKHLPPGALIDHEG
                     VPTLDPKVMLEAPFGSLTPFGGHKGFGLAAMCEIFAGALSGGFTTHKDTLGTTNAIIN
                     CMLSVIVDPAAFDAPDAQAEADAFIDWVKASPLAAGADRIYEPGEPERVMRAQREAEG
                     VPVDPATWAQIRAAALAVGMSAEQAERWSAKLQ"
     gene            complement(2273..3736)
                     /locus_tag="VHV99_02005"
     CDS             complement(2273..3736)
                     /locus_tag="VHV99_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX3634262.1"
                     /translation="MISDDTTQQTQRINQDTLRDFVDRKWNDEIVPALTDYIAVPAKS
                     PAFDPEWVKHGYLERVITDAAQWAEQQPVRGLKLEVIRLPGRTPVIFFETAATRSGSE
                     ETIVLYGHLDKQPEFDGWRNDLGPWTPKLENDKLYGRGGADDGYAIYASLTALAALDA
                     QGIERPRCVGLIETCEESGSYDLLPYVDALRERLGKVGLVVCLDSGAGNYDQLWLTTS
                     LRGLVAGDLEVQVLDEGIHSGGYGGIAPSSFRIMRQLFDRLEDSANGTLLPKGFHVQI
                     PAERLREAEATAQILGDDVWKKLPWACGQDGRQVLPTTTDPQEALLNSTWRPSLSVTG
                     AAGLPALADAGNVLRPRTAFKLSLRLPPLIEAEKAVAELKALLELDPPYNAKVTFKPD
                     AGAASGWSAPDLAPWLATALNDASRAHYGADVAYMGQGGTIPLMNVLKSGFPKSQFMV
                     CGVLGPKSNAHGPNEFLHVPYGKKLTAAVAQVIAAAP"
     gene            3923..4234
                     /locus_tag="VHV99_02010"
     CDS             3923..4234
                     /locus_tag="VHV99_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superinfection immunity protein"
                     /protein_id="HEX3634263.1"
                     /translation="MSGIVVLQAIAAVFALALYFLPSILADRRKRHDLLTLALFNACL
                     GWTGFGWLLALYWSLQPNPPKNVAGEVAETRKIMRMRAFSSALLVRVQRRATARERGE
                     K"
     gene            complement(4271..4543)
                     /locus_tag="VHV99_02015"
     CDS             complement(4271..4543)
                     /locus_tag="VHV99_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YodC family protein"
                     /protein_id="HEX3634264.1"
                     /translation="MLTATIEEFDSPPAARFNVGDVVTLKEGGSRMTVTYAGPVALNP
                     GDWLICEWFDEHGELRREMFAHDSVRAEPRSIPAGSVLWSRMSRAA"
     gene            complement(5045..6622)
                     /locus_tag="VHV99_02020"
     CDS             complement(5045..6622)
                     /locus_tag="VHV99_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3634265.1"
                     /translation="MNSEAIKPAPAAFVEPAARPAAGSAGVAVCPGVGAVAATKVAAG
                     SAAAAGKAASGFERQGLALAVLFVGAFLAPLDYFIVNLALPSIHTGLNATDAQLQLVV
                     SAYASAYAVLLITGGRLGDLFGRRRMFMAGMAAFVVASALCGFATSGHMLVISRIVQG
                     IAAAVMAPQVLATIRAVVPLHRQTKVMSLYGFVFGLSSIVGQLGGGALITYRPFGLDW
                     RIIFLINIPIGIVAFIGTWKFVPENQPATSTGIDLKGVALLSIVLLLVIYPMTHGREA
                     GWPAWTFVMFALAVPAFALFVAAERRVERGGGHPLVDLQLFRNPAFALGLVLAFLFYC
                     NSAFFLTYGIFLQTGLHWTPLASGMAIMPFAVGFVVGPLTSPAVVKRIGGHVLALGFS
                     MMTIGFTVTGWSATHAATPDLLFYCGLVCAGVGHGLLLPSIMRIVLLEVAPEKAGLAS
                     GVVSSTLQIGSAFGTAAISGAFFGVIGGRVSPDAYAHAFQFSLAINACLMFVCIGLSV
                     LLVRHQQLALRRAAQLV"
     gene            complement(6653..>7274)
                     /locus_tag="VHV99_02025"
     CDS             complement(6653..>7274)
                     /locus_tag="VHV99_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX3634266.1"
                     /translation="SKQSAKKAGEVCPRGRPREFDTDTVLASASQVFWNHGYHATSID
                     DLCKATGLLRGSLYGVFGDKHGIMLAALEHYAEGSVARLAERLNAPVPAEEALRNALL
                     HYARVACALTGERSCFITNATLEMQPDDEELRTRVAAIQRRMATLLAASVIRGQASGA
                     FNSALDEKAVGDFLLCVMQGLRVLGRVAHQEDALIGIVDVAMRALV"
BASE COUNT         1475 a         2285 c         2264 g         1250 t
ORIGIN      
        1 cgttcatctt cggtcggagc gtgtcgataa aggtgcgcgt cgccatatag gcgtcgcccg
       61 gttcgaacag ctggtcgacc agccccctgc cgaagtgcca ttcagccgta tgcgactccc
      121 ccacgccgat caattcctcg gccagacgca tgcccgcctt gcgcgcgacc aatgaataac
      181 cgcccatgcc cgggaacaaa ttgaacgcga tctccggaaa tcccatgcgc gcgtcgctct
      241 gcgccagcag aaaatgatgc gcgagcgccg cctcgaagcc gccgcccagc gccgtgccct
      301 ccaccatcgc gatcgaaatc gcccccgtat cgaagccgcg ggccgcgcca tgcacacaat
      361 cgacgcaggc gcgcgcgtac gccatcagcg tctggcgctt cccggcgcgg atcgtctcgg
      421 cgaagaaatc gaggtcgccc ccgacgttga acatggtcgg gatcaacgag ccggtcaccc
      481 agaagtcgat cggcaagcct gactctcgtg ctgcctgcgc gagacccaga atgtcgtgca
      541 ccagatcgag attgaaacag ggacgcggct gcgcccgcag catcatccac atgatgtttc
      601 ggccttcttc atagtaagcg gagacttgcg acaaattgcc ggcttcgaga aacgggcggc
      661 aggcgtggtg attatgcaag ttcattttga tcggtccttt tagggttaat cactggcatt
      721 gcaatgcgag cctaaagcag accggaatag gttgggagcg gggtaaccaa cagatgaatg
      781 caacacacgc gtgaagcccc gtcagacggc gctctgcggg gaggaaaact gcgcaccaaa
      841 agatgcagga tgcgcaaacg gttgcgcacc ggagatgggc gaaattaacc gcaaaaggaa
      901 tacgttgccg caataaatta gcgcacgaac aaagctggaa tttcttgccg gatttgaaag
      961 agaaagaaag gtcttcgatt aggcaatgca atcgaaatat agcgtgtaga tgattgtttt
     1021 tttatttgtg ggacaacaga cggaatattc aatttgaata attaaatgcg ggcgcggcaa
     1081 agcgcccgct atttgcaagc aatgaatgct attgcagctt cgcggaccat cgctcggcct
     1141 gctccgcgct catgccgacg gctaatgccg ccgcgcggat ctgcgcccac gtagccggat
     1201 cgacgggcac gccctcggcc tcgcgttgcg cgcgcatcac ccgctccggt tcgcctggct
     1261 catagatgcg gtccgcgccg gccgcgagcg gcgaagcctt cacccagtcg atgaacgcat
     1321 cggcctcggc ttgcgcgtcg ggggcatcga acgcggccgg gtcgacaatc accgagagca
     1381 tgcagttgat gattgcgttg gtcgtgccga gcgtgtcctt gtgcgtggtg aagccacctg
     1441 agagcgcacc ggcgaaaatc tcgcacattg ccgcgagccc aaaacccttg tgaccaccga
     1501 acggcgtcaa cgagccgaac ggcgcttcga gcatgacttt gggatcgaga gtcggaacgc
     1561 cctcgtgatc gatcagcgcg ccgggcggca gatgcttgcc ctggttatag gcgacgcgct
     1621 ctttgccgta agccaccgtg ctggttgcga agtcgagcac gagcggcggc ttaccgggcc
     1681 gcggatacgc cgcgcaaaac ggattcgtgc cgatgcggcg gtccgcgccg ccaaatggcg
     1741 cgacgagcgg gtcgcccgcc acattgacga aatggaagga gacgaggccg gcgcgtgcgc
     1801 attgttcggc ccagtggccg atgcgcccga tatggtgcgc gctgcgcaaa ccgacggcgc
     1861 agatgccgaa gcgttgcgcg cgctcgatgc cctgctccat cgcctcgaac gcgacaacct
     1921 gaccgaagcc cctcgcgcct tcgacggtca ggaccgcgcc cgcgtccttc acgacccgcg
     1981 cgtgcaggtt cagttgcagt tgcgcgtcgg ccaacgacgc cacataacgc gggatcatcc
     2041 ccacgccatg cgaatcgtga ccggtcagat tcgccgctac cagatgatcc gcaaccagcc
     2101 cggcttcgcg cggcgtgctg cccgcctgct cccagattgc ccgcacgtag gcgtgcagtt
     2161 gatcggccgg aatccggagc gaggtgggtt cagcagtcgg gttgtcggtc attcacagta
     2221 ccggcgtgat tggaaaaggt cactgcgcga tcaacacggc accagaacag gatcaaggcg
     2281 cggcggcgat cacctgggcc acggctgccg tcagcttctt gccgtacggc acgtgcagga
     2341 actcgttggg cccgtgcgca ttcgacttcg gtccgagcac gccgcacacc atgaactgcg
     2401 acttcgggaa acccgacttc agtacgttca tcaacgggat cgtgccgccc tgccccatgt
     2461 aggcaacgtc ggcgccgtaa tgcgcgcgcg acgcgtcatt gagcgccgtg gcgagccacg
     2521 gtgcgagatc gggcgcgctc cagccgctcg ccgcgccggc gtccggcttg aaagtcacct
     2581 tggcgttgta cggcggatcg agctcaagca gcgctttcag ctcggcgacg gccttttccg
     2641 cttcgatcag cggtggcagg cgcagcgaca gcttgaacgc cgtgcgcgga cgcagcacgt
     2701 tgccggcgtc ggcgagcgcg ggcaagccgg ccgcgccggt caccgacagc gacggacgcc
     2761 acgtcgagtt gagcaaggct tcctgcggat cggtggtggt gggcagcacc tgacggccgt
     2821 cctggccgca ggcccacggc agcttcttcc agacgtcgtc gccgagaatc tgggcggttg
     2881 cttccgcttc gcgcagacgc tccgccggga tctggacgtg gaaccccttc ggcagcagcg
     2941 tgccgttggc ggaatcttcg agacggtcga acagttgccg catgatgcgg aaactcgacg
     3001 gcgcaatccc gccgtaaccg ccggagtgaa tgccttcgtc gagcacctgg acttcgaggt
     3061 cgccggcaac cagaccgcgc agcgaggtgg tgagccagag ttgatcgtaa ttgcccgcgc
     3121 ccgagtcgag acagacgacg agaccgactt tgcccagccg ctcgcgcaac gcgtcgacgt
     3181 acggcagcag atcgtagctg cccgattcct cgcaggtttc gatcaggccg acacagcgcg
     3241 gacgctcgat gccttgcgcg tccagcgcgg ccagcgccgt gaggctcgcg tagatcgcgt
     3301 agccgtcgtc ggccccgccg cgaccgtaca gcttgtcgtt ttcgagcttc ggcgtccagg
     3361 ggccgaggtc gttgcgccag ccgtcgaact caggctgctt gtcgaggtgg ccgtacagca
     3421 cgatggtttc ttcgctgccc gaacgggtcg cggccgtttc gaagaaaatc accggcgtgc
     3481 ggcccggcag gcgaatcact tcgagtttca gaccgcgcac gggttgctgt tcggcccatt
     3541 gcgccgcgtc ggtgatcacg cgctcgagat aaccgtgctt gacccactcg ggatcgaacg
     3601 ccgggctctt ggccggcacc gcaatgtagt ccgtgagcgc gggcacgatc tcgtcgttcc
     3661 atttgcggtc tacgaagtcg cgtagcgtgt cctggttgat gcgctgcgtc tgctgggtcg
     3721 tatcgtcgga gatcatggtg gcggtccgtg gcggttctgt tcagtcgaaa cgtccatgat
     3781 agtcccgatg cgcgatcggc tgaacccctt gcggtgcatt ccatcacagc ggctattcgc
     3841 aacggacgct cacgccgaat ctgcgccagc aaaacaatcg cgcgacaatc gcagtatgga
     3901 tcgcgtcctg gggagccgta ctatgagtgg catcgtcgta cttcaggcca tcgccgcggt
     3961 gttcgcgctg gcactgtatt ttctgccgag cattctcgcg gaccggcgca agcgccatga
     4021 cctgctgacg ctggcgctct tcaatgcgtg cctcggctgg acgggttttg gctggctgct
     4081 ggcgctctac tggtcgttgc aaccgaaccc gccgaagaac gtagccggcg aagtggcaga
     4141 gacgcggaaa atcatgcgga tgcgggcgtt ttcgtcggcg cttctggtgc gtgtgcaaag
     4201 gcgtgcaacg gcgcgtgagc gcggggagaa ataatcgagc cgagaatcag gctgcaaatc
     4261 aggctgcaaa tcaggccgcg cggctcatcc tgctccacag caccgagccg gcgggaatcg
     4321 agcgtggctc ggcccgcacg ctatcatgcg cgaacatctc gcggcgcaat tcgccgtgtt
     4381 cgtcgaacca ctcgcaaatc agccagtcgc caggattgag cgctaccgga cctgcgtaag
     4441 tcacggtcat gcgcgagcca ccttctttca atgtgacgac gtcgccgaca ttgaaacgtg
     4501 cggctggcgg tgagtcgaat tcttcaatgg tcgcggtcag catattcaat cccctacgga
     4561 acccgttgtg tggaattaag tctcgcatcg tgctttgcaa cattgaacct gccgaaggaa
     4621 aactttaaaa agtttgccaa gattaacaat ggaaattaac cacggcaagt gctttttatt
     4681 gaaacctttt tcatcgacgg tggaataagt ttgcttgccg cgcgagcgcc aggtggactc
     4741 ggataaaccg ctcagaacgg cgccaagtcc cgacccagca gcccctgcgc gcaagcaaaa
     4801 tttacggccg caagctcatc cgcaagtaga aaatgcgtga tccatttgtc atatctaaag
     4861 cagccactga cagagcagga tcagggttta ttctaatctc aggcaaacgg ggaattacct
     4921 gtgaacggaa aactcatcgg taaatgcaga cctcaaaaac cgggttttgt ttaaagtaca
     4981 taataatttt cgggagccgc cgttgaaaaa ggagcgaatg aaaacgattt cgggccgcta
     5041 tttttcaaac cagctgtgca gcgcggcgca atgccagttg ttgatgccgc accaagagca
     5101 cgctcaaacc gatacacacg aacatcagac acgcgttgat cgcgaggctg aactggaagg
     5161 cgtgcgcata ggcatccggg ctcacgcggc cgccaatcac gccgaagaac gcgccgctga
     5221 tggccgccgt gccgaacgcc gaaccgatct gcaaggtcga cgacacgacg cccgaagcta
     5281 gccccgcctt ctccggcgcg acttccagca gcacgatgcg catgatcgac ggcagcagca
     5341 ggccgtgacc gacgcccgcg cacaccagtc cgcaatagaa cagcaggtcg ggcgtcgcgg
     5401 catgggtcgc cgaccagccg gtgaccgtga agccgatcgt catcatcgag aagcccaacg
     5461 ccagcacatg gccaccgatg cgcttgacca ccgcgggcga tgtcagcggc ccgaccacga
     5521 agccgaccgc gaacggcatg atggccatgc ccgaggcgag cggcgtccag tgcaaaccgg
     5581 tttgcaggaa gatcccgtag gtcaggaaaa acgcgctgtt gcaatagaac aggaacgcga
     5641 gcaccaggcc cagcgcaaaa gccggattgc ggaacaattg caggtctacc agcgggtggc
     5701 cgccaccgcg ctcgacgcgc cgttcagccg ccacgaatag cgcgaacgcg ggcacggcaa
     5761 gcgcgaacat cacgaaggtc catgctggcc agccggcttc gcggccatgt gtcatcggat
     5821 agatgacgag cagcaacacg atcgacagca acgcgacgcc cttcaggtcg atgccggtgc
     5881 tcgtggccgg ctgattttcc ggcacgaact tccaggtgcc gataaaggcc acgatgccga
     5941 tcggaatatt gatcagaaag atgatgcgcc agtcgagccc gaacggacga taggtgatca
     6001 gcgcgccgcc gccgagctgg ccgacaatcg acgaaagccc gaacacgaaa ccgtagagac
     6061 tcatgacctt ggtctgccga tgcagcggta cgaccgcgcg gatggtcgcg agcacttgcg
     6121 gcgccatgac cgcggcggca atgccctgca cgatccgcga gatcaccagc atgtggccac
     6181 tggtggcgaa gccgcacagc gcggacgcga ccacgaacgc cgccatcccc gccatgaaca
     6241 tgcgacgccg gccgaacagg tcgccaaggc gcccgccggt gatcagcaac acggcgtaag
     6301 cggaggcgta ggcggacacc accagctgca attgcgcgtc ggtggcgttc aggccggtgt
     6361 gaatcgacgg caacgcgagg ttgacgatga aatagtcgag cggcgcaagg aaggcgccca
     6421 cgaacagcac ggcgagcgct aagccctggc gctcgaagcc ggacgcagcc ttcccggcgg
     6481 cagcggccga gccggcggca accttggtgg cggccactgc ccccacgccg gggcagacgg
     6541 cgacgcccgc actaccggct gcgggccgcg ccgccggttc aacgaatgcc gctggagcgg
     6601 gcttgatcgc ttcgctattc atgactgaac gttcaaaaat tagacaaaaa atttagacga
     6661 gcgcgcgcat agcgacgtcc acgatgccga tcagcgcgtc ttcctgatgc gccacccgcc
     6721 ctaatactcg cagcccttgc atcacgcaca gcaggaaatc gccaacggct ttttcgtcca
     6781 gcgcggaatt gaaggcgccg ctcgcctgcc cgcgaatcac cgacgctgcc agcaaggttg
     6841 ccatgcggcg ttgaatggcc gcgacgcggg tgcgcagttc ctcgtcgtcg ggttgcattt
     6901 ccagcgtggc gttggtgatg aagcagctgc gctcgccggt cagcgcgcag gcaacccgtg
     6961 cgtaatgcag caaagcgttg cgcagcgcct cttccgccgg caccggcgcg ttcagccgct
     7021 cggcgagccg cgcgacggaa ccttcggcgt agtgttcgag tgcggcgagc atgatgccgt
     7081 gcttgtcgcc gaatacgccg tacaggctgc cgcgcaacag gcctgtggcc ttgcagaggt
     7141 cgtcgatcga ggtggcgtgg tagccgtgat tccagaaaac ctggctcgcg ctcgccagta
     7201 ccgtgtccgt atcgaactcg cgcggtcggc cccgagggca cacctcgccc gcttttttgg
     7261 cggattgctt cgaa
//
LOCUS       DATASH010000070         6897 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6734355,
            whole genome shotgun sequence.
ACCESSION   DATASH010000070 DATASH010000000
VERSION     DATASH010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6897
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6734355"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..3064
                     /locus_tag="VHV99_02030"
     CDS             143..3064
                     /locus_tag="VHV99_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011853975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX3634267.1"
                     /translation="MEHHARTQDERLEVKTTTCYMCACRCGIRVHLREGEVRYIDGNP
                     EHPLNQGVICAKGASGIMKQYSPARLTQPLMRKAGAERGSAQFEPVSWEVAFDVLEKR
                     LATIRATDPKKFALFTGRDQMQALTGLFAKQFGTPNYAAHGGFCSANMAAGMIYTIGG
                     SFWEFGGPDLDSAKLFFMIGTAEDHHSNPLKIAISKFKRAGGRFIAINPIRTGYAAIA
                     DEWVPIKPGTDGALFMAFLHELIAADAWDHEFVQRYTNAAELVDLDEGSENFGLFVRD
                     PERPAGNPLFPQNHLWWDAEAARAVPHHSPGVTPALDGRYTLDDGTPVTPSFALLRER
                     VADCTPEWAAGITGIAADTIRRLAGEMIQTSRDHRITLPIRWTDAWGETHESVTGNPV
                     AFHAMRGLAAHSNGFQSIRALAVLMSLLGTIDRPGGFRHKSPFPRAVPPSAKPPNSPA
                     AVKPNTPLASGPLGWPAAPEDLFIDEHGGPVRIDKAFSWEYPLAVHGVMHSVITNAWR
                     GDPYPIDTLLIFMANMAWNSSMNTMKVREMLVDKHANGEYKIPFLVVCDAFQSEMTAF
                     ADLILPDTTYLERHDAMSMLDRPISEFDGPVDSVRVPVVPPTGECKPFQEVLIELASR
                     LKFPAFTTAEGTRRFRDYPDFIVNHTTTPDSGVGFLIGWRGKDGDKALVGEPNPQQWE
                     QYAKNNCVFHYRLPEAMQYMRNCNGPYLDWAVEKGFRKFNEPILIQLYSDVMQKFRLA
                     AQGRTKGRQPPEHLRARVEKYFDPLPFWYAPLESDSTDLARFPLAAVTQRPMAMYHSW
                     DSQNAWLRQIHGENYLYMNPLMAAENGITDGSWIYAESQWGRVRCMARFSETVEPGTV
                     WTWNAIGKASGAWNLGAGANESQHGFLLNHLITDELPGRDEAAARLSNSDPVTGQAAW
                     YDVRVRIYPAEADARHTLPQFDAMPALPGSNGVISRVVQTYFAGRGEFAARLRGAVGR
                     K"
     gene            3201..3947
                     /locus_tag="VHV99_02035"
     CDS             3201..3947
                     /locus_tag="VHV99_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX3634268.1"
                     /translation="MTQMALVIDLNVCVGCQACVTSCKEWNTSGESGSLADLNPYDAD
                     PSGTFFNRVQSFEAGSYPNAETIHFPKSCLHCEDPPCVPVCPTGASYKRKEDGLVLVD
                     FDKCIGCKYCAWACPYGARELDESRKEMTKCTLCVDRIHDENLSERDRQPACVLACPT
                     SARLFGDIHDPESVVSKAIEERGGYQLMPEWNTRPANHYLPRATTLASGCGSGACSCK
                     DAGTAQETPESLDAQLEHGKLHLASIATRI"
     gene            3986..4936
                     /locus_tag="VHV99_02040"
     CDS             3986..4936
                     /locus_tag="VHV99_02040"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DmsC/YnfH family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX3634269.1"
                     /translation="MNPAFSVVFLTTLSGAAQGLLIALVGVETAAHLGLVMAPADAFY
                     VTGAALTLVLGGLGLIASFFHLGHPERAWRAIAMWRTSWLSRECLCLPAFLACAFFYA
                     VAHWFGSPWSLAIGWLGVLASAVLFVCTAMIYACLRFLQEWATPLTLVNFVLLGCASG
                     FTLATALSAWFAPGLTAGLAICACVLTLAGCASRTASLVRNARLRPKSTVQSATGIKN
                     PKLVQVSRGFTAGAFNLREFFHGKSAGTLRGVKWGFLTAAFVVPIVLIALGAGLHSIS
                     VSLTLLAAACLVQYAGLVAERWFFFAEAKHPQNLYYARVG"
     gene            4998..5225
                     /locus_tag="VHV99_02045"
                     /pseudo
     CDS             4998..5225
                     /locus_tag="VHV99_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920277.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            5950..>6897
                     /locus_tag="VHV99_02050"
     CDS             5950..>6897
                     /locus_tag="VHV99_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011547494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX3634270.1"
                     /translation="MKLQRTKRRWLFVPALLLASAAHAQSSVTLYGLIDEGLNFTSNA
                     QGHSAYQMKSGDFYGSRWGVKGSEDLGGGYRAIFQLENGFNASNGALGQDSREFGRQA
                     FVGISSDRYGTLTFGRQYDPTSDGNSFGALTAAGNWGGDLAAHPFDNDNADWDFRPNN
                     SVKYVSPTYRGLTAEGMYAFSNEAGGFSNNSLYGGSVNYQNGGLTAAASYMKLNHPGG
                     NTSGAVVGDTLFTGSSEQNYGAGVSYKFSKTLLAFVYSHVDVYNPTANSYFTSNTGPD
                     GGTWNSWKFDNFEINDQYFFRPDFWLGTAYTFTDARLASS"
BASE COUNT         1311 a         2080 c         2141 g         1365 t
ORIGIN      
        1 atattgaagg caaattgaaa ccatcagcgc agcggatagt ttctctaata aatcgcgatt
       61 tcccttgtgg gccggtggcg aataggatgg tcggtattga accaaccgct tcgacaccac
      121 cgtcgcagcg acggagatcc gtatggaaca ccacgcccgc acccaggacg aacgactcga
      181 ggtcaagacc accacctgct acatgtgcgc gtgccgctgc ggcattcgcg tgcatttgcg
      241 cgaaggcgag gtgcgctata tcgacggcaa tccggagcat ccgctcaacc agggggtgat
      301 ctgcgccaag ggtgcctcgg gcatcatgaa gcagtattcg cccgcgcgcc tgacccagcc
      361 gttgatgcgc aaggcgggtg cggagcgggg cagtgcgcag ttcgaaccgg tgtcgtggga
      421 ggtcgcgttc gacgtgctcg aaaagcgcct cgccacgatt cgcgccactg atccgaagaa
      481 attcgcgctg tttaccgggc gcgaccagat gcaggcgctc accggcctct tcgccaaaca
      541 gttcggcacg cctaattacg cggcgcacgg cggtttctgc tcggccaaca tggcggccgg
      601 catgatctat acgatcggcg gctcattctg ggaattcggc ggccccgatc tcgacagcgc
      661 caaacttttc ttcatgatcg gcacggcgga agatcatcat tcgaatccgc tcaagatcgc
      721 tatttcgaag ttcaagcgcg cgggtgggcg cttcatcgcg atcaatccga tccgcaccgg
      781 ctacgcggcg attgccgacg aatgggtgcc gatcaaaccc ggcaccgacg gcgcgctgtt
      841 catggcgttt ctgcacgaac tgatcgcggc cgacgcctgg gatcacgagt tcgtgcagcg
      901 ttatacgaac gcggcggaac tcgtcgatct cgacgagggc agtgagaact tcggcctgtt
      961 cgtgcgtgac cccgagcggc ccgccggcaa tccgctcttt ccgcagaatc atctctggtg
     1021 ggatgccgaa gccgcgcgcg ccgtgccgca tcattcaccc ggcgtgacgc ctgctcttga
     1081 cggacgctat acgctcgacg acggcacgcc agtcacgccg tcattcgccc tgctgcgcga
     1141 acgcgtggcc gactgtacgc ccgaatgggc tgccggcatt accggcatcg cggcggacac
     1201 gattcgccgc ctcgcgggcg aaatgatcca gaccagccgc gaccaccgga tcacgctgcc
     1261 gatccgctgg accgacgcgt ggggcgagac ccacgagagc gtcaccggca acccggtcgc
     1321 ctttcacgct atgcgcgggc tggccgcgca ctccaacggc ttccagtcga tccgcgcgct
     1381 ggcggtgctg atgtcgctgc tcggcacgat cgaccgcccc ggcggctttc gtcataagtc
     1441 accgtttccg cgcgcggtgc cgccctcggc caaaccaccg aacagccccg ccgccgtcaa
     1501 gccgaacacg ccgctggcaa gcggcccgct cggctggccc gccgcgcccg aagatctgtt
     1561 catcgacgaa cacggcggcc cggttcgcat cgacaaggcc ttctcctggg aatatccgct
     1621 cgccgtgcat ggcgtgatgc atagcgtgat caccaacgcg tggcgcggcg atccgtatcc
     1681 catcgacaca ttgctgattt tcatggccaa catggcgtgg aattcgtcga tgaacacgat
     1741 gaaggtgcgc gagatgcttg tcgacaagca cgcgaacggc gagtacaaga ttccgtttct
     1801 ggtggtgtgc gatgcgttcc agtcggagat gacggcgttc gccgatctga tcctgccgga
     1861 cacgacctat ctcgagcggc acgacgcgat gtcgatgctc gaccggccga tctccgaatt
     1921 cgacggtccg gtggattccg tacgcgtgcc ggtcgtgccg ccgacggggg agtgcaagcc
     1981 gttccaggaa gtgctgatcg aactcgcttc acggctgaag tttcccgcct tcaccaccgc
     2041 cgaaggcacg cggcgttttc gcgactatcc cgacttcatc gtcaatcaca ccacgacgcc
     2101 tgattcgggc gtcggctttc tgatcggctg gcgcggcaag gacggcgaca aggcgctggt
     2161 gggcgagccg aatccgcagc agtgggaaca gtacgcaaag aacaactgcg tgttccatta
     2221 ccggttgccc gaggcgatgc aatacatgcg caactgcaac gggccgtatc tcgactgggc
     2281 ggtggaaaaa ggcttccgta aattcaacga gccgattctg atccagctgt actccgacgt
     2341 catgcagaag ttcaggctcg ccgcgcaagg ccgcacgaag ggcaggcagc cgcccgagca
     2401 tctgcgggca cgggtcgaaa agtatttcga tccgctgccg ttctggtatg cgccgctcga
     2461 aagcgattcc accgatctcg cgcgcttccc gctcgccgcc gtgacgcaac ggccgatggc
     2521 gatgtaccac tcgtgggatt cgcagaatgc atggctgcgg cagattcatg gcgagaacta
     2581 tctctacatg aatccgctga tggcagccga aaacggtatt accgacggca gctggatcta
     2641 tgccgaatcg caatggggcc gggtgcgctg catggcgcgc tttagcgaga ccgtggagcc
     2701 gggaacggta tggacctgga acgcgatcgg caaggcttcg ggcgcgtgga atctcggcgc
     2761 gggcgcgaac gaatcgcagc acggcttcct gctcaatcac ctcattaccg atgagttgcc
     2821 cggtcgcgac gaagcggccg cacgcctctc gaattcggac ccggtgacgg gccaggccgc
     2881 gtggtacgac gtgcgggtgc ggatctatcc ggcggaagcg gacgcgcggc ataccctgcc
     2941 gcagttcgac gcgatgccgg cgttgccggg atcgaatggg gtgatatcgc gggtcgtgca
     3001 gacctatttc gcgggacgcg gcgaattcgc ggcgcggctg cgtggtgcgg tggggcgtaa
     3061 atagcacacg gatggcacgt gagcagtgag ccggcggcag cgcgcgagcg gcgcgcacgt
     3121 agcgggcgag tagcgaacag gtggtgcacg agcggcagat aagtggcgca cccgttacgc
     3181 atgggaagaa ggagcgattg atgactcaga tggcattggt gatcgacctg aacgtatgcg
     3241 tgggatgcca ggcttgcgtg acgagctgca aggagtggaa cacgtcgggc gagtcgggca
     3301 gtctcgccga tctgaacccg tacgacgccg acccgtccgg caccttcttc aatcgcgtgc
     3361 aaagcttcga agcgggcagc tacccgaacg ccgagacgat ccacttcccg aagtcatgcc
     3421 tgcattgcga ggacccgccg tgtgtgccgg tttgcccgac cggcgcgagc tacaagcgca
     3481 aggaagacgg cctcgtgctg gtcgacttcg acaaatgcat cggctgcaaa tattgcgcgt
     3541 gggcgtgccc gtacggcgcg cgcgaactcg atgagtcgcg taaggagatg accaaatgca
     3601 cgctctgcgt cgatcgtatc cacgatgaaa atctctcgga gcgcgatcgc caaccggcat
     3661 gcgtgctcgc ctgtccgacc tccgcgcggc tgttcggcga tatccacgat cccgagtcgg
     3721 tagtgtcgaa ggcgatcgag gaacgcggtg gttatcagtt gatgcctgag tggaacacgc
     3781 gtccggcgaa ccactatttg ccgcgcgcga cgacgttggc atccggttgc ggcagcggcg
     3841 cgtgctcgtg caaagacgcg ggtacggcgc aggagacgcc tgaatcgctc gatgcgcagc
     3901 ttgagcacgg caagctgcat cttgcgtcga tcgcgacgcg catctaaggc atccaaggca
     3961 tctaaggcat ctaaaggact tcacgatgaa tccggctttt tcggtagttt ttctcactac
     4021 gttgagcggc gcggcgcagg gtctgttgat cgcgctcgtc ggcgtggaga cggccgcgca
     4081 tctcgggctc gtcatggcgc ccgcggacgc gttctatgtc accggcgcgg ccttgacgct
     4141 cgtgctcggc gggctcggac tgattgcctc gttcttccat cttgggcatc cggagcgcgc
     4201 atggcgagcg atcgcgatgt ggcggacctc gtggctgtcg cgcgaatgtc tatgcctgcc
     4261 ggcatttctc gcctgtgcgt ttttctacgc tgtcgcacac tggttcggct cgccgtggtc
     4321 attggcaata ggttggctcg gcgtgctggc gagtgccgtg ctgttcgtct gcacggcgat
     4381 gatctatgca tgcctgcgtt ttctgcagga atgggccacg ccgctcacat tggtcaattt
     4441 cgtgctgcta ggctgcgcgt ccgggttcac gctggcgacg gcgctgagcg catggttcgc
     4501 gccgggactg acagcgggcc tggcgatctg cgcttgcgtg ctgacgcttg ccggttgcgc
     4561 gagccgcacg gcatcgctgg tgcgcaatgc gcgattgcgg ccgaagtcga ccgtgcagag
     4621 cgcgaccggc atcaagaacc cgaagctcgt gcaggtctcg cgcggcttta ctgccggcgc
     4681 gttcaacctg cgcgagtttt ttcacggtaa atcggccggg acactgcgcg gcgtcaagtg
     4741 gggatttctg acggcggcgt ttgttgtgcc tattgtgttg atcgcgctag gcgcgggatt
     4801 gcattcgatc agcgtgtcac tgactttgct ggccgccgcg tgtctcgtgc agtacgcggg
     4861 tctcgttgcg gagcggtggt tcttcttcgc cgaggcgaag cacccgcaga atctgtatta
     4921 cgcgcgagtg ggatgatgca tgcgtgcgcg cgaccgcctg attgaaggct gacggcttac
     4981 ttccggccga cactgaagtc agatcctcga agtatctgaa taacctgatc gagcgggacc
     5041 atcgaaacat caggtcccgg acgaaggtca tgcttggcct caagcggttt aagcgcgccg
     5101 ccaccacgat ttcaggcatc gagttgacgc atcgcatgcg caaggggcag ttcaatctct
     5161 ggacgctcga cctcaaaagc accgctatgc cgaccgtttg gaatgcgctc ctttcggttc
     5221 cgtaagtcat cctgtcaaaa ataaaacttc ttcggctgcg tgcactattt gcaccggagc
     5281 cctcccgcac ggcctcttgc agccgcgcat ggatctggtc ttcgtggtaa gccttgtccg
     5341 atacgacggc gggatcagtg aaggttgctc ctaacaaaat caaacgacag ccttacctat
     5401 tgcatacaat ttaggatctg tctgtctact tccttgcttg caatctatcg gcagggcatc
     5461 gagaaggcat ctgcaagctt gcgccccttg ctctcgcacg gcgcgccgca ctggtgctgc
     5521 attgcactgt cggtgggatt ctcgaaaagc cccggcgcat tcttggctta acgcaacgac
     5581 gccggcccag cgccgtcaaa gctgcgtctt cattgcgtac caagacaaat tactttgcat
     5641 acaattcttc ataagaatgt tggcatgatc attgcgttgt ggattcagct ttcttttcga
     5701 ctttcctgac gcccgcacat gaaacaacgc ccttctctga aagatttacg accgcgcttc
     5761 ggcgttggac gcggagtgga agactcccat gacgcggttt gatggtcaac gccggttgaa
     5821 agcgccaggt ggagtgcggt cctcccggat caataacccg ccttatcaaa attaacacct
     5881 cgactatttc ccctaattgc accgaagtgg ttggcgatag gatattcacc acgtagtaga
     5941 aggaacgtca tgaaattgca gcgaacaaaa cgtcgatggc tatttgtccc cgcgctgctg
     6001 ctcgccagcg cagcccatgc gcagagcagc gtcacgctct acgggttgat cgacgagggc
     6061 cttaacttca ccagtaacgc gcagggccac agcgcttatc aaatgaagag cggcgacttt
     6121 tacggcagtc gctggggcgt gaaaggcagc gaagatctcg gtggtgggta tcgggcgatt
     6181 ttccagttgg agaatgggtt caatgccagc aatggggcgc ttggtcaaga ttcacgcgaa
     6241 ttcggccgcc aggcatttgt cggcatctct tccgaccgct acggcacatt gacgttcggc
     6301 cgccaatacg atccgacctc ggacggcaac agcttcggcg cccttacggc tgctggtaac
     6361 tggggcgggg atctcgcggc gcacccgttc gacaacgaca acgcggattg ggacttccgc
     6421 ccgaacaatt ccgtcaagta cgtgagtccg acttaccggg gactcaccgc cgagggcatg
     6481 tatgcgttca gcaacgaggc cggcgggttc tccaacaaca gcctctatgg cggcagcgtc
     6541 aactatcaga atggggggct gacggcggcg gcatcgtata tgaagctgaa tcaccccgga
     6601 gggaacacat ccggcgctgt ggtcggcgat accttgttca cgggttcttc ggaacagaac
     6661 tacggcgccg gggtgtcgta taagttctcg aagacgctgc tggcctttgt ctactcgcat
     6721 gtcgacgtgt acaaccccac ggctaactcc tacttcacgt cgaatacggg ccccgacggc
     6781 ggtacgtgga actcgtggaa attcgacaac ttcgaaatta acgaccagta cttcttccgg
     6841 ccggactttt ggctaggtac cgcctatacc tttacggacg cgcgcctggc atcgagc
//
LOCUS       DATASH010000071         4822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_602432,
            whole genome shotgun sequence.
ACCESSION   DATASH010000071 DATASH010000000
VERSION     DATASH010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4822
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602432"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VHV99_02055"
     CDS             complement(<1..190)
                     /locus_tag="VHV99_02055"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051860.1"
                     /note="composes the biotin carboxyl carrier protein
                     subunit of the acetyl-CoA carboxylase complex, the enzyme
                     that catalyzes the carboxylation of acetyl-CoA to
                     malonyl-CoA, which in turn controls the rate of fatty acid
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEX3634271.1"
                     /translation="MDLRKLKTLIDLVSESGISELEVTEGEGKVRIVKNAPPVYVQPS
                     ASYAPQYAQPAPLPGGEAP"
     gene            complement(267..722)
                     /gene="aroQ"
                     /locus_tag="VHV99_02060"
     CDS             complement(267..722)
                     /gene="aroQ"
                     /locus_tag="VHV99_02060"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006756005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEX3634272.1"
                     /translation="MTRLLVLHGPNLNLLGTREPEVYGRVTLPQIDQALADRAADAGV
                     ELASFQSNHEGALVDRIQSARTETTDFILINPAAYTHTSVAIRDALAGVGIPFVEIHL
                     SNVHRREPFRHHSYFSDQAEGVICGLGWKGYLYALEFALDRLAAGSSRG"
     gene            complement(917..1465)
                     /locus_tag="VHV99_02065"
     CDS             complement(917..1465)
                     /locus_tag="VHV99_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX3634273.1"
                     /translation="MNMKRILAGALVAIAAAGGGVLANYWLNGDGEVAHAAQSASTQS
                     AVEQLWAAPVTNVDGKPQTLSLLKGHPIVVNFWASWCGPCVEEMPALSQLQRDYAKKG
                     IQFVGLGVDSDKNVQAFLQKVKVAYPVYVTGFGGADLARAFGNTAGGLPFTVVIDAKG
                     NIRSTKLGQINPQALKQTLDAL"
     gene            complement(1481..2134)
                     /locus_tag="VHV99_02070"
     CDS             complement(1481..2134)
                     /locus_tag="VHV99_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--alanine ligase"
                     /protein_id="HEX3634274.1"
                     /translation="MVRKSHFDPQRVREEIAIAAARMIAEDGLDYATAKRKAARQVVG
                     ESRVAGEWLPDNDQIEEEIREYQSLFQGDSQPALLRRLRHLAIDWMERLAPFNPYLTG
                     AVLGGTAGEHSDIHLQVFCDNPKEVAIYFLNANIQYDVSETRHFAGRGYVETLSFLWR
                     PTNEARDAEPVGIHVALYETDDLRGAVRADARGRTARANQQAVQALLDSSDVTSPTN"
     gene            2346..3740
                     /gene="mpl"
                     /locus_tag="VHV99_02075"
     CDS             2346..3740
                     /gene="mpl"
                     /locus_tag="VHV99_02075"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEX3634275.1"
                     /translation="MHIHILGICGTFMGGLAVLARGAGHTVTGCDAGVYPPMSTQLEA
                     QGIRLIEGYDAEQLNGLNADLFVIGNVVSRGNPLMEAILDRGLPYVSGPQWLGEHVLN
                     GKWVLAVAGTHGKTTTSSMLTWLLEDAGLNPGFLIGGVPLNFGVSARLTDSSFFVIEA
                     DEYDTAFFDKRSKFVHYRPKTAVLNNLEFDHADIFPDLAAIETQFHHLIRTVPGLGRI
                     VTNGRENALERVLTRGCWSEVERFGVQGGWETLPEEDGEPVDERFAVYHNSERVGVVE
                     WQVQGEHNRMNALAAIAAARHIGVPPAVAAKSLASFRNVKRRMEVRGSVDGVTVYDDF
                     AHHPTAIETTVAGLRARVGRENTRILAVLEPRSNTMKLGVMKAQLPASLADADLVFGY
                     GAPAGRDALGWNLGEALAPLGGRAQAFDNLDALVKAVVHAARPGDQILVMSNGGFGGV
                     HQKLLDALSARGTS"
     gene            3737..4339
                     /locus_tag="VHV99_02080"
     CDS             3737..4339
                     /locus_tag="VHV99_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HEX3634276.1"
                     /translation="MILYLHGFRSSPNSFKARVLAARLVELGRSDEWCCPTLPVSPLE
                     TVALAESLVAAAQPDNVTVIGSSLGGYFATHLAEKHGWPAVLLNPAVVPQRDLSAYLG
                     EQPLWHGGGSITVELHHLDELRALGVASITRPERYYLIAATGDEVLDYREMLAHYPGA
                     RTTLIEGSDHAISEFAQYVDDVLAFCDAEDVEPPAPREAA"
     gene            4523..>4822
                     /locus_tag="VHV99_02085"
     CDS             4523..>4822
                     /locus_tag="VHV99_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEX3634277.1"
                     /translation="MNVFFEESGSFKAGSVLSRQGDAFQVELPGGRRAKVRAKDVLIE
                     FEKPSAGELMQQADAAAQDIDLDFLWECAPEDEFPFATLGAEYFGESFGSIERAAL"
BASE COUNT          857 a         1491 c         1526 g          948 t
ORIGIN      
        1 ccggggcttc accgcctggc agaggagccg gctgggcgta ttgcggcgca tacgaagcgg
       61 acggttgcac gtaaaccggc ggcgcattct tgacgatgcg gaccttgcct tcaccttcgg
      121 tcacttcgag ttcggaaata ccggactccg agacgaggtc gatcagagtt ttcagtttac
      181 gtagatccat caggaagccc ctttcaatgc gtaaagtgcc ggcgcgtgca atcgcagtac
      241 ccggcttaaa ttggacgtgt ttggaatcag ccgcgcgacg aaccggcggc aagccggtcg
      301 agcgcgaatt cgagtgcgta caggtatccc ttccagccga gcccgcaaat cacgccttca
      361 gcctggtcgg agaaatacga gtgatgccgg aacggttcgc ggcgatgtac gttcgacaga
      421 tgaatctcga cgaacgggat gccgaccccc gccagtgcat cccgaatggc cacgctggtg
      481 tgcgtgtacg cggcgggatt gatcagtatg aaatcggttg tttcagtccg ggcggattgg
      541 atgcgatcga cgagtgcccc ttcatgattg ctctggaacg acgccagttc gacacctgcg
      601 tccgcagcgc ggtccgccag cgcctgatcg atctgcggca acgtcacccg gccgtagacc
      661 tcaggttccc gggtgccgag aagattgagg ttggggccgt gcagtaccag cagtcgcgtc
      721 atggtcgctt ctttttccgt ggaattgggc gcactttagc gctgattaga gaaacttgtc
      781 tagtttcctg cacgttcgac atggcctcgg cggccatttg ccgctgctcc gggcgcccat
      841 cgtctctcaa atttaagcga tttgcgcgta atttcgcgcc ttttgcctct atctgatcgt
      901 caaacgaatg tgaaatttag agcgcgtcga gcgtctgttt cagcgcttgc gggttgattt
      961 gccccaattt tgtcgagcgg atattgcctt ttgcgtcgat gacaacggta aacggcaggc
     1021 cgccggcggt attgccgaac gcgcgcgcca ggtcggcgcc cccgaatccg gttacataga
     1081 ccggataggc cacttttacc ttttgcagga acgcctgcac atttttatcc gaatcaacgc
     1141 caagtcctac gaattggatg ccttttttcg catagtcgcg ctgaagttgc gaaagcgcgg
     1201 gcatttcctc gacgcacggg ccgcaccacg acgcccagaa gttgacaaca attgggtggc
     1261 ctttaagcaa acttagcgtt tgcggcttgc cgtcgacatt ggtgacaggc gcggcccaca
     1321 actgctcaac ggcgctttga gttgaagcgc tctgcgccgc atgggctact tcgccgtccc
     1381 cgttcagcca gtagttggcc agcaccccgc cgcccgccgc ggcgatcgcg accagcgcgc
     1441 ctgccagaat ccgtttcatg ttcattccag ctctgtctaa ttaattagtt ggagaggtga
     1501 cgtcgctact gtccagcagc gcctgaaccg cctgctgatt cgcgcgggcc gtgcggccgc
     1561 gcgcgtcggc ccgtaccgcg ccgcgcaagt cgtctgtttc gtacagggcg acgtggatgc
     1621 cgacaggctc cgcatcccgc gcttcgttcg tggggcgcca caggaagctc agcgtctcga
     1681 cgtagccgcg accggcaaaa tgccgcgtct cagacacgtc gtattgaatg ttggcgttca
     1741 gaaagtagat cgcgacttct ttcgggttgt cgcagaacac ctgaaggtga atatccgagt
     1801 gttcgccggc cgtgccgccg agcaccgcgc ccgtcagata cggattgaac ggtgcgagcc
     1861 gctccatcca gtcgatcgca aggtggcgta accgccgcaa cagcgccggc tggctgtcac
     1921 cctggaaaag cgactgatat tcacggattt cttcttcgat ctggtcgtta tccggcagcc
     1981 attcgccggc aacacggctc tcaccgacta cctgccgtgc cgctttacgc ttggccgttg
     2041 cgtagtcgag accgtcttca gcgatcatcc gggcagccgc gatggcgatt tcctcgcgca
     2101 cgcgctgcgg atcgaaatgt gatttgcgaa ccatctctca atcatactag agattgatga
     2161 aagccctttc tgggcggttc gcagcgggtg cctggcgtgg gcggggcggg cggccaccct
     2221 gtggcccggt tcatagggcg atggaaaggc gtcggttaca atagcgtcct ttgcagacgg
     2281 tcgtttcggc cgcttctctg cgcttccatc ccttgcaaag tacgcccgcg cggcacgaca
     2341 ggcttatgca catccacatc ctcggtatct gcggcacgtt catgggcgga ctcgcggtcc
     2401 tcgcgcgcgg cgcgggccac accgtgacgg gctgcgacgc cggcgtttat ccgcccatga
     2461 gcacgcagct cgaggcgcaa ggcattcgtc tgatcgaagg ttacgacgct gaacagttga
     2521 acggcctcaa cgccgatctg ttcgtgatcg gcaacgtggt ctcgcgcggc aacccgctca
     2581 tggaagcgat cctcgatcgc ggcctgcctt acgtttccgg tccgcaatgg ctcggcgagc
     2641 acgtgctgaa cggcaagtgg gtgctggcgg tggccggtac ccacggcaag accaccacca
     2701 gttcgatgct gacgtggctg ctcgaagacg cgggtctcaa tccgggcttc ctgatcggcg
     2761 gtgtgccgct gaatttcggc gtctcggcgc ggctgaccga ttccagcttc ttcgtgatcg
     2821 aagccgatga gtacgacacg gcctttttcg acaagcgttc caagttcgtt cactatcgcc
     2881 caaagaccgc ggtcctgaat aatctcgaat tcgatcacgc cgacatcttc ccggatctgg
     2941 ccgcgatcga aacgcagttt catcatctca tccgcacggt gccgggcctc ggtcgcatcg
     3001 tgacgaacgg tcgggaaaac gcgctggagc gcgtgctgac gcgcggttgc tggagcgaag
     3061 tcgagcgctt cggcgtgcaa ggcggttggg aaaccttgcc ggaggaagac ggcgagccgg
     3121 tcgacgagcg tttcgccgtg tatcacaaca gcgagcgcgt cggggtggtc gagtggcagg
     3181 tgcagggtga gcacaaccgc atgaacgcgc tggccgcgat cgccgccgcg cggcatatcg
     3241 gcgtgccgcc ggccgtggcc gcgaagtcgc tggcgagctt ccgcaatgtg aagcgccgca
     3301 tggaagtgcg cggcagcgtc gacggcgtga ccgtctacga cgatttcgcc catcacccca
     3361 cggcgatcga aaccacggtg gccgggctgc gcgcgcgcgt cggccgcgag aacacgcgca
     3421 tcctggccgt gctcgagccg cgctcgaaca ccatgaagct cggcgtgatg aaggcgcaac
     3481 tgccggcgag tctggccgac gccgatctgg tgttcggcta tggtgcgccc gctggccgcg
     3541 acgcactcgg ctggaatctc ggcgaagcgc tcgcgccgct gggcggcagg gcgcaggctt
     3601 tcgacaatct cgacgcgctg gtcaaagcag tcgtccacgc agcgcgcccg ggcgaccaga
     3661 ttctggtaat gagcaacggc ggcttcggcg gcgtgcatca gaagctgctc gacgcactct
     3721 ccgcgcgagg cacttcgtga tcctttatct gcacggtttc cgttcgtcac ccaattcgtt
     3781 caaggcgcgc gtgctggcgg cccgcctcgt cgaactgggc cgcagcgacg aatggtgctg
     3841 tccgacgctg ccggtctcgc ccctcgagac cgtggcgctc gccgaatcgc tggtggcggc
     3901 agcgcaaccg gacaatgtca cggtgatcgg cagttcgctg ggtggctact tcgccacgca
     3961 tctcgccgag aagcacggct ggccggcggt gttgctgaac ccggcggtcg tgccgcaacg
     4021 cgacctgagc gcctacctcg gcgagcagcc tttgtggcac ggcggcggca gcatcaccgt
     4081 cgagctgcat catctggatg aattgcgcgc gctcggcgtc gcgtcgatca cccggcccga
     4141 acgctattat ttgatagccg ccaccggcga cgaagtgctc gattaccgcg aaatgcttgc
     4201 gcactatcca ggcgcgcgca ctacgctaat cgaagggagc gaccacgcga tcagcgaatt
     4261 cgcgcaatac gtcgacgatg tgctggcctt ttgcgatgcc gaagacgtcg agccgccggc
     4321 gccgcgcgag gcggcctgac agtcacggcc gcgacgattg cgccgcgagg ccgttgagta
     4381 tcaccagaat catgccgccg gcgcgcgcgt atcgcgccgc ggatccgcta ccacgaacac
     4441 gttgccgtgc cgcgcgcgca gtgaccgata gctgaaccca atcgggcgcg ccggcagatg
     4501 caagtcagaa cgagagtatt gagtgaacgt tttcttcgag gaatcgggca gtttcaaggc
     4561 gggcagcgtg ctgtcgcgcc aaggcgatgc ttttcaggtc gagttgccgg gcggccgccg
     4621 cgccaaggtg cgcgcgaaag atgtgctgat cgaatttgaa aagccgagcg ccggcgagtt
     4681 gatgcagcag gctgacgccg ccgcgcagga catcgatctg gattttctgt gggaatgcgc
     4741 gccggaagat gaattcccgt ttgccacgct gggcgccgag tatttcggcg aatcgttcgg
     4801 ctcgatcgag cgcgcggcgc tg
//
LOCUS       DATASH010000072         2806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4954212,
            whole genome shotgun sequence.
ACCESSION   DATASH010000072 DATASH010000000
VERSION     DATASH010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4954212"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VHV99_02090"
     CDS             complement(<1..432)
                     /locus_tag="VHV99_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012400064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX3634278.1"
                     /translation="MAITYKTPDDITKLRISGRLAADVLAMIGEHVKAGVSTDELDAL
                     CNDYIVNTQKSIPANVGYLGFPKTVCTSVNQVVCHGIPSRSEILKDGDIINIDVAIIK
                     DGYFGDTSRMYCVGQPSTVARQLIDTTYEAMLAGIREVKPGA"
     gene            823..1800
                     /locus_tag="VHV99_02095"
     CDS             823..1800
                     /locus_tag="VHV99_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX3634279.1"
                     /translation="MSESNNKSAELIDVLDRRVERRLQRRQFFRNAGGLGLGLVGGTL
                     ISACGGGSGSSVSAASAPTDTEILNFALNLEYLESQFYTYATTGSALPASMTAGVGTM
                     GMVIPGQQVPFQDPVVQAYANEIANDEREHVNFLRTALGSAAVAMPSIDIGGTNPNGA
                     FSNAARAAGLVGAGVAFNPYANDNNFLLGAFIFEDVGVTAYKGASPLITNKTFLEAAA
                     GILAAEAYHAGLVRTVLFAKGVDTANIYTAANAISAARDSLDNNGHDDQGITGATAGS
                     SNIVPLDSNGLAFSRGYGNVLNIVYLTSSMATKGGFFPNGVNGTLNMSA"
     gene            complement(2042..2554)
                     /locus_tag="VHV99_02100"
     CDS             complement(2042..2554)
                     /locus_tag="VHV99_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HEX3634280.1"
                     /translation="MRALAAIVLLLWATAACADWHGDVQSAKLVGEGDFSLFGFRLYR
                     AQMWSERVPVDYDERFALHIIYVRGIKRERFVDAGMNEIKRLASVPISADTLARWRAD
                     MLQAFVDVEPGDQLSAVYLPDKGVRFYAGERMTGEFDDLAFARAFFGIWLDPSTRAPI
                     LRRQLLGMPQ"
     gene            complement(2556..>2806)
                     /locus_tag="VHV99_02105"
     CDS             complement(2556..>2806)
                     /locus_tag="VHV99_02105"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3634281.1"
                     /translation="RRFIEAARRGKLAARTSLTFGRDYAETLRRWRSAFEAQLEPIRA
                     QGFDEIFVRTWRLYLAYCEAGFDEGRTGVMQFVLASAD"
BASE COUNT          544 a          887 c          850 g          525 t
ORIGIN      
        1 ggcgcccggc ttgacctcgc ggatgcccgc cagcatcgct tcataggtcg tgtcgatcag
       61 ctggcgcgcg accgtgctcg gctgaccgac gcaatacatg cggctggtgt cgccaaaata
      121 gccatctttg atgatcgcga catcgatatt gatgatgtcg ccgtctttca ggatttcgct
      181 gcggctggga atgccgtggc acaccacctg gttgaccgac gtgcagacgg ttttcggaaa
      241 tcccagatag ccaacgtttg cgggaatcga cttttgcgta ttgacgatgt agtcgttgca
      301 cagggcgtcg agctcgtcgg tggagacgcc ggccttgacg tgctcgccga tcatcgccag
      361 tacgtcggcc gcgaggcggc cggaaatgcg cagcttggtg atgtcgtcgg gagtcttgta
      421 ggtaatggcc atgaattcgg tcggggtaaa tcgatgttga cgatcggcca attgtacaga
      481 ggatcgccgg acggcctgtg caagccgggc cggaactgtt cgatggatgc tgtgcgcgcg
      541 gcgccgggcc gcgttcggca tcgtgcagcc atggtccgtt caatcgcatg ctgcgagcgc
      601 acacagaaat cttttgggag ctttgcatcc ggcagcgcac tcgctccgta aagacgattg
      661 aggactcact tcttctgcct gcatcgtcta gttcgtctca gatgaacggg ggcctctttt
      721 gcgtccgcga tttgcgtttg tgcatcgtgt gagtatccgt tgtccacgca tatacaccgc
      781 gcgtccgcgg cattggcagc gaaccatctg aggagcatcg ccatgtcgga gtccaacaat
      841 aaatcagcag agcttatcga cgtacttgac cgccgggttg aacggcgcct gcaacgcagg
      901 cagtttttcc gcaacgcggg tggtttgggt ctagggttgg tcggcggtac gctgatcagt
      961 gcgtgcgggg gtggttccgg ctcgagcgtg tcggccgcca gcgcgccgac cgatacggag
     1021 attctgaact tcgcgctgaa tctggaatac ctcgaatcgc agttctatac gtatgcgacg
     1081 accggatcgg cgctgcccgc gagcatgacc gccggcgtcg gcacgatggg catggtgatc
     1141 ccgggacagc aagtgccgtt ccaggatccc gtggtgcaag cctacgcgaa cgagatcgcc
     1201 aacgacgaac gcgaacacgt caactttcta cgcacggcct tgggtagcgc agccgtggcg
     1261 atgccgtcga tcgacatcgg cggcaccaat ccgaacggtg ccttctcgaa cgcggcgcgg
     1321 gcggcgggac ttgtcggcgc gggtgtcgcc ttcaatccgt atgcgaacga caacaacttc
     1381 ctgctcggcg cattcatctt cgaggacgtc ggcgtgacgg cctataaggg cgcttcgcca
     1441 cttatcacca acaagacctt cctcgaagcg gcggccggca ttctcgcggc ggaagcgtat
     1501 cacgcgggtc tggtacgcac ggtgctgttt gcaaaaggcg tcgacacggc aaatatctac
     1561 acggccgcca acgcgatctc cgccgcgcgc gacagcctcg acaacaatgg ccacgacgat
     1621 cagggcatca ccggcgcgac ggccggttcg tcgaacatcg tgccgctcga cagcaacggg
     1681 ctcgctttta gccgcggcta tggcaacgtg ctcaacatcg tgtatctgac gagctccatg
     1741 gcgaccaagg gtggtttctt cccgaacggt gtgaatggca cgctcaacat gagcgcctga
     1801 aacacgcagg ctgctgtgcc agtctgtgcc agttgcaccg gcaacgggtc gccgcgatga
     1861 cgatcgcgct cacccgttgg ccggccttca tccgcattgc cttgaacgct tgaagacgga
     1921 caggagcagt cgtaaacgaa gcgatgagtc aggccgccgt gcctcggtcc ctcaccgagc
     1981 ggcgcgacgt ggtcgaaaac agcctggcac cgcaagttcc ccatgcgcat cgcgcgtacc
     2041 ctcactgcgg cataccaagc aactgcctgc gcaaaatcgg cgcgcgcgtc gacggatcga
     2101 gccagatgcc gaaaaatgcc cgtgcaaatg caagatcgtc gaactcgccg gtcatccgtt
     2161 caccggcata gaaacgcacg cccttatccg gcagatacac cgcgctcaac tgatctcccg
     2221 gctccacgtc gacgaacgcc tgcaacatat ccgcgcgcca gcgcgccaac gtatccgccg
     2281 aaatcggcac gctcgcaaga cgcttgattt cgttcatacc cgcgtcgacg aatcgttcgc
     2341 gcttgatgcc ccgcacgtaa atgatgtgca gcgcaaagcg ttcgtcataa tcgacgggca
     2401 cgcgttcgct ccacatctgc gcgcgataga gcctgaagcc gaacaggctg aaatcgccct
     2461 cgcccacgag tttcgcggac tgcacatcgc cgtgccaatc cgcgcaggcg gccgttgccc
     2521 acaacagcag cacgatagcc gcgagcgcgc gcatgtcagt ccgccgaggc gagcacgaac
     2581 tgcattacgc ccgtgcgccc ttcgtcgaag ccggcctcac agtacgccag atacagccgc
     2641 cacgtccgca cgaaaatctc atcgaagcct tgcgcgcgga tcggctcgag ctgagcttcg
     2701 aacgcactgc gccagcggcg cagcgtctcg gcgtagtcgc gcccgaatgt caacgacgtg
     2761 cgcgccgcga gcttgccgcg ccgagccgct tcgatgaacc gccgcg
//
LOCUS       DATASH010000073         3262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2580887,
            whole genome shotgun sequence.
ACCESSION   DATASH010000073 DATASH010000000
VERSION     DATASH010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2580887"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..726
                     /locus_tag="VHV99_02110"
     CDS             118..726
                     /locus_tag="VHV99_02110"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-70"
                     /protein_id="HEX3634282.1"
                     /translation="MAQVSGPALDDPVYLTQLRRDLLRFARLQLRDAAAAEDAVQEAL
                     AAAWTQADRFAAQSEHKTWVFGILRHKLVDTLRARQRTVNLSALESEIDGEALLDREL
                     FKDNGHWSREAKPQPWPAPETALRQQQFWTLFEMCLEHLPEHIGRVFMMREFLDLETE
                     AICAELQMTANHCSVLLYRARLRLRTCLSEKGLSREDANGEV"
     gene            713..916
                     /locus_tag="VHV99_02115"
     CDS             713..916
                     /locus_tag="VHV99_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX3634283.1"
                     /translation="MGKCKNITRLLSDALQRHLTTGEWVAIRLHLPICSGCRNYRKQI
                     RLLRVAAHTASGIATPGVGGRDD"
     gene            complement(1021..1311)
                     /locus_tag="VHV99_02120"
     CDS             complement(1021..1311)
                     /locus_tag="VHV99_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HEX3634284.1"
                     /translation="MLELRPSCEGCGKSLPPNASDAMICTYECTFCEVCALSTLRNVC
                     PNCGGNFQHRPIRTRAQLAKHPARAEAHPVSIDEATHAGFFERYRNTPPGER"
     gene            complement(1447..2823)
                     /locus_tag="VHV99_02125"
     CDS             complement(1447..2823)
                     /locus_tag="VHV99_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015295 - solute:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate:H+ symporter"
                     /protein_id="HEX3634285.1"
                     /translation="MHLTTTLAPWSSHDTQLILSCALGLALIIIFISVLKLAPFLSIL
                     VGTFAAGFSAGLPLEAVASAFSKGAGALLGDVGIIIALGAMLGALMAESGAADRLVST
                     ILKHSTPRTLPWMMALVAIIVGLPLFFEVGLVMMVPIIFVMARRSQQPILRIAIPALA
                     GMTTLHALLPPHPGPLIAVSALHADLGLTLGLGLIVAIPAVILAGPVYGIWLSKRMRV
                     AEPEEMGKLFTAPQNTGEPPSFAISLSTILLPVVLMLGRTVAKLLLQPETFLFNALNF
                     LGEPLVALGLTVLFAVVALGWSRGMARDRVGGILRKSLPPIAALLLTIGAGGGLKQAL
                     VVAGISTTIGKIAVGAHMPLILLAWLIAVALRQATGSATVATTTTAGIVAPVVAGLSA
                     THNSLMALAIGAGSVFFCHVNDAGFWMVREYFGLQLKQTVMVWSVLQTIVSVVGLALT
                     FVLWTALT"
BASE COUNT          644 a         1018 c         1051 g          549 t
ORIGIN      
        1 cgtgcgggag aatggcgcac acgccccaga accgcgcgaa gattcccact tgcctatgag
       61 ccacaacgat tcaggagaaa acctgcggca accacgcacg ccgcgaggga gcctgccatg
      121 gcgcaagtaa gcggaccggc gctcgacgat cccgtctatc tgacgcaact gcggcgcgac
      181 ctgttgcgct tcgcccgttt gcaactgcgc gatgccgctg ccgccgaaga cgcagtccag
      241 gaggctttgg ccgccgcctg gacgcaagcc gaccggttcg ccgcgcaatc ggagcataag
      301 acgtgggtgt tcggcatcct gcggcacaag ctcgtcgaca ccttgcgcgc ccggcagcgc
      361 acggtgaacc tgtcggcgct cgaatccgaa atagatggcg aggcgctgct cgatcgcgaa
      421 ctcttcaagg acaacggcca ctggtcgcgc gaggccaagc cgcaaccctg gccggcacca
      481 gaaaccgcgc tgcgtcagca gcaattctgg accttgttcg agatgtgcct ggaacacctg
      541 cccgaacaca tagggcgcgt gttcatgatg cgcgaattcc tcgatctcga aaccgaagcg
      601 atctgcgcag aactgcaaat gaccgccaat cactgcagcg tgctgctcta ccgcgcgcgg
      661 ctgcgcttgc gcacttgcct gagcgaaaag ggcctgtcga gagaggatgc gaatggggaa
      721 gtgtaagaac atcacgcgtc tgttgtcgga cgcgctccag cgccatttga cgactggcga
      781 atgggtcgcg atccggctgc atttgccgat ttgcagcggc tgccgcaatt accgcaaaca
      841 gatccgcctg ttacgcgtgg ctgcgcacac ggcaagcggg attgcgacgc cgggggtagg
      901 cggccgcgac gattgaggcg gtgtccgggt gtctggactt gagcggtgcg tcgaacgaca
      961 gccccgcggg ttatagcgga cggcgactgc attgaacgcc gccttcggcg attggctcgt
     1021 ttaacgctcg ccaggcggtg tgttccggta acgctcgaag aagccggcgt gtgtcgcttc
     1081 atcgatggaa acgggatgcg cttcggcacg cgccggatgt ttggccagct gcgcacgcgt
     1141 gcggatcggc cgatgctgga aattgccgcc gcagttcgga cacacattgc gcagcgtgct
     1201 gagcgcacac acttcacaga acgtgcattc gtacgtgcag atcatcgcgt cggacgcgtt
     1261 cggcggcagt gatttgccgc atccttcgca ggacggtctc agttcaagca tggtggctcc
     1321 gtgttgactg gggtacgagg tgcgggactg cacctcgcca tgccgaagca gtgtaagcgg
     1381 acagggtcat gcggaatgga cagcggagcg tcaattcctg ccacgcctgc aatccctatg
     1441 cggggctcag gtcagcgcgg tccacaacac gaacgtcaat gcaaggccaa caacagagac
     1501 gatggtctgc agcacggacc agaccatcac cgtttgcttc aattgcaggc cgaagtattc
     1561 gcgcaccatc cagaagcccg cgtcgttcac gtggcagaag aacacggaac cggcgccgat
     1621 agcgagcgcc atcagcgaat tgtgcgtcgc gctgagacct gcgacgacgg gcgcgacgat
     1681 accggcggtc gtggtggtcg caaccgtcgc ggaaccggtg gcctgccgca gcgccacggc
     1741 gatcagccac gccagcagaa tcagcggcat gtgcgcgccg acggcgatct tgccgatcgt
     1801 cgtactgatg ccggccacca cgagcgcctg cttgagtcca ccgcctgcgc cgatggtcag
     1861 cagcagcgcg gcgatcggcg gcaagctctt gcgcagaatg ccgccgaccc ggtcgcgcgc
     1921 catgccgcgc gaccagccca gcgccactac cgcgaacaac accgtgagac cgagcgcaac
     1981 caacggctca cccagaaaat tgagcgcgtt gaacaggaaa gtctcgggtt gaagcagaag
     2041 tttggcaacg gtgcggccca gcatcagcac gaccggcaac aggatcgtgc tgagcgagat
     2101 cgcgaaactc ggcggctcgc cggtgttttg tggcgcggtg aaaagcttgc ccatttcttc
     2161 cggttcggcc acgcgcattc gtttcgacaa ccagatgccg tagaccgggc ccgcgagaat
     2221 gaccgccgga atcgccacga tcagaccgag accgagcgtc aggccgaggt cggcgtgcag
     2281 cgcgctcacg gcaatcagcg gacctgggtg cggcggcagc agcgcatgca gtgtggtcat
     2341 gccggcgagc gcgggaatcg caatccgcag gatcggctgc tgcgagcggc gcgccatcac
     2401 gaagatgatc ggcaccatca tcaccagacc gacctcgaag aagagcggca agccgacgat
     2461 gatcgccacc agcgccatca tccacggcag cgtgcgtggc gtggaatgtt tgaggatggt
     2521 cgagacgagc cggtcggcgg cgcccgattc ggccatcagc gcaccgagca tcgcgccgag
     2581 cgcgatgatg atgccgacgt cgccgagcaa cgcaccggcg cctttgctga acgcgctggc
     2641 taccgcttcc agcggcagac cggccgaaaa tcccgccgcg aacgtgccga ccagaatcga
     2701 cagaaacggt gcgagcttca gcacgctgat gaaaatgatg atcagtgcga gcccgagcgc
     2761 gcacgacaaa atcagttgag tgtcgtggga tgaccacggc gcgagtgttg tcgtcagatg
     2821 cacgatgtcc cttttttcga ttgacgcagc cgcgactgcg cgcggatgac ggattgtagc
     2881 gggacgcagc gccttcacgg agcctgacac gcaacgaggc ggcgcaggta agtcctgtgc
     2941 ctgccggcgt gatttgctta tgcgaattta tcgggtcgtc caggtttgca gacgaattat
     3001 cgtcagagcg aatcggtccg ctttgcgtcc gtctttttaa tgcgcacttt tatgtcccgt
     3061 catccgctgc tcagccgccg cgtgtttatt gccggcgtgg gcgcgtcgct ggccgctgcg
     3121 gccttgcccg ttgtctccac gtctgtcttc gccgcggacg cccacgcgaa cgaatttcgc
     3181 atcggtttac agcgaagcag cggctcagct cgcgccgatt caagggctcg acgcaggcgt
     3241 gatcgaagcg ggtttgcggc ac
//
LOCUS       DATASH010000074         6320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4756540,
            whole genome shotgun sequence.
ACCESSION   DATASH010000074 DATASH010000000
VERSION     DATASH010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6320
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4756540"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..694)
                     /locus_tag="VHV99_02130"
     CDS             complement(122..694)
                     /locus_tag="VHV99_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006400759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HEX3634286.1"
                     /translation="MNQAFLISTGAVTLAEIGDKTQLLSLVLAARYRKPIPIILGVLT
                     ATLVNHACAGALGTWLGSLLTPTIMRWALAASLIAMGLWILVPDKLDDEEANTNRTHF
                     GVFGATVVTFFLAEMGDKTQIATVALAARFHDFFGVVAGTTLGMMIANVPAILLGDRF
                     AHKLPTRTVHAIAAVMFVILGTMALFGVGV"
     regulatory      complement(714..923)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00080"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="yybP-ykoY riboswitch is a manganese riboswitch that
                     directly binds Mn; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="manganese"
                     /db_xref="RFAM:RF00080"
     gene            1552..2385
                     /locus_tag="VHV99_02135"
     CDS             1552..2385
                     /locus_tag="VHV99_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HEX3634287.1"
                     /translation="MPDLHWTIPVARWSSWPAAAAAAPDIGFIEPIVRRRLSTLSRVA
                     LKVAHDCVAQDDIRVVFASRHGELRRTTEILHAISAGEPVSPTAFSLSVLNAMTGVFG
                     IARGDRSAASAISAGAETLGYALLEAYAQYTAQPGSPVLLVYADEPADPAYGTIEDEV
                     QGGAIAILLDGEAAMGQLVCTLSGAGEPEPAKTAEEAGHAAEAGHADEASHAAEASHA
                     GKASQAGKPTHAIEPGAALPAESFATQTQALLHCLETGKPAVWPGAGAAWHWRWHERA
                     P"
     gene            2369..3163
                     /locus_tag="VHV99_02140"
     CDS             2369..3163
                     /locus_tag="VHV99_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX3634288.1"
                     /translation="MNARLDYLWRFFATGMAFVVFGICGLLFSVLVFPLAWLWPHRAS
                     RQLAVTTVIHWFFRALVAVLQRIGVMELEVSGAEALRAGGPAIVVANHPTYLDVMVLL
                     SLTPRACCVVKNAHWGNPCFWGIVRSAEYVSNADPSELVEAGARQLAAGYTMIIFPEG
                     TRSPAPNRLHAFSRGFAHMALKVGAPIVPVLMDCDPPAFTKQMRWYDVPARAFRMRVN
                     VLAPFGVERLADHDPSPSLAARSVTSAIEAHITQHLFDYGFFKTGN"
     gene            3138..3437
                     /locus_tag="VHV99_02145"
     CDS             3138..3437
                     /locus_tag="VHV99_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEX3634289.1"
                     /translation="MDSLKLEIKQLLIEALDLEDLSPADIDDDAPLFDTDGIGLDSID
                     ALEIGIVLRKQYQLTIAANDERTRDHFRSISTLAALVASQREAVHAVNETTKKGD"
     gene            3440..3691
                     /locus_tag="VHV99_02150"
     CDS             3440..3691
                     /locus_tag="VHV99_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX3634290.1"
                     /translation="MSEAEILERIRAIFKENFAIEPERVTPDAHLFEDLDLDSIDAVD
                     LAIKLQEMTGRRIKPEEFKSVRTVGDVIGAVESLLAAQG"
     gene            3691..4353
                     /locus_tag="VHV99_02155"
     CDS             3691..4353
                     /locus_tag="VHV99_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634291.1"
                     /translation="MPSVRSRMQATASQPGEAQPGTERHWAKTVVQVLLKLAYPALIL
                     CAWRWDTPRYVGCMLFVILWLQRWAGSGPVATSLRRLSTIDWTVVGLLSCASAAIVFT
                     NSELLLRLYPSLVNLGLLIAFGATLGRGPSMIEKFARLGNPNLSPGAVRHTRRVTQVW
                     CVFFALNGAFSAYTALYWSRASWSLYNGAIAYGLIGVLLVAEIIWRYLVVLPRAARSE
                     AA"
     gene            4350..6122
                     /locus_tag="VHV99_02160"
     CDS             4350..6122
                     /locus_tag="VHV99_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX3634292.1"
                     /translation="MIALHELLLADREASAMNAPVCRDGATVLDRAAFRARVSALVAL
                     MQTQDAQRYALCIDDPFDFACALFALFACGKEPVIPANATPGYLADLADAYDAVLTDA
                     DLAHGVRDAGADADDKQDKRDKQDKPDNPARGTSHTIDPQAPLTLYTSGSSGTPKPIR
                     KTLAQFNAEVHTLEKQWGALIGDATMLASVPHHHIYGLLFRVLWPLAAGRAFERAISI
                     EPLHLQAQIQTQIQAQIAPCGATVVVSTPAQLSRWPALPGFAALTPAPRAFFSSGGPL
                     AVEAAQEYATAYGAAPIEIYGSTETGGIAWRRQDQTDAWQSVTGIEIRCDEDGALNVR
                     SPHLDHDGWHRTDDKIAVDADGRFRLQGRLDRVLKLDGKRVSLPELEARLALHPYVAQ
                     AAIVPLAGASRERVGAVVALTEAGSEALRGEGRVLLAKTLRRHLAAYFDLVVLPRHWR
                     FRLTLPFDARGKLPVAAVAAAFEARADGVEVLAEARSADTLHYELRVPPALVHFAGHF
                     PGLPILPGVVQVHWAMHLATEHVPAVRALASIDRLKFMAPVPPGAVLNLTLAHDAERR
                     RVQFAYRLSGRECASGVIVYREPA"
     gene            6122..>6320
                     /locus_tag="VHV99_02165"
     CDS             6122..>6320
                     /locus_tag="VHV99_02165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3634293.1"
                     /translation="MSFAPCIVIPIYNHKDAIGSTVAHLAVHGLPIFVVDDGSDEATQ
                     QVLAGLARQYAGQLTLLRLPVN"
BASE COUNT         1113 a         1983 c         2034 g         1190 t
ORIGIN      
        1 gctgttgtgt cgaacagcag acgttgcggc gcaacaaaaa aacggcccgg caggtcattc
       61 ctggcgggcc gttttgcttc gcgagcaacg cgcgagctga gcgctgcatg agtggacttg
      121 cttaaactcc cacgccgaac agcgccatcg tccccagaat cacgaacatc accgcggcga
      181 tggcgtgcac ggttctggtc ggaagcttgt gggcgaagcg gtcgccaagc aggatcgccg
      241 gtacgttggc gatcatcatg ccgagcgtcg tgccggccac cacgccgaag aaatcgtgaa
      301 agcgcgcggc gagggccacg gtcgcaatct gcgtcttgtc gcccatttcc gcgaggaaga
      361 acgtcaccac ggtcgcgccg aacacgccga aatgcgtgcg gttggtgtta gcttcttcat
      421 cgtcgagttt gtccggcacg agaatccaca agcccattgc aatgagcgac gcggccaatg
      481 cccagcgcat gatggtcggc gtgagcagcg agccgagcca ggtaccgagt gcgccggcgc
      541 aggcgtgatt gacgagcgta gcggtcagca cgccgagaat gatcggtatc ggcttgcgat
      601 agcgggcggc gaggacgagc gagagcagtt gcgtcttgtc gccgatctcg gcgagcgtga
      661 ccgctccggt cgaaatgaga aaagcttgat tcacggtgtt tttgttcctc gggccgaggt
      721 gtgaacgagc gacgacccac aaccccgccg ggccgctgtt gcggcgtgtg tggatcatcg
      781 gtctcgccag gccagaggct gcggctgcca tggccataaa ggccaagttt gttgacaggc
      841 gcccacgcga gccacacgtt cgacgcaccg tatgacagcg gtgcggacat gccgcggggc
      901 ggctactccc caatgaggga cggagtatag caggcgtagt tgcacgttac gcgccaattc
      961 cgcgatggcg ctgctgctcc ctgcgcaaat ggcattgcgc taagcgctcg tcagtcgtgc
     1021 catgggattt gaacgccaca tgggcgtata acgcagcgca tcactcatcg gaaaggcacc
     1081 gaagccttca aatgttccga aacgaaacat ctattcagaa agttaaatga aagcatttgc
     1141 atcgacttgt ggatacaaca ccgctcggtt ggccggcaga gcgcggtgcg agtggcctgc
     1201 cagccccatc ccggatgcac caggcgccac caggcctgca ttgcggcgtg tccagtgatt
     1261 cgtcgcacct gcgatacgga tacagataga tacgtctttt gtggctgatt taatccagaa
     1321 tattcagtat gattccgccg accataggga gcccgagcca atcctgcaga ccaattggca
     1381 aaggcgccta aataaccggg gccaagtgcc gttaaattgc tgaaccctat ataagaaact
     1441 ggattgaccc ccggctggag attgccgttc cgcgcggtgc gcacgctgtg acggccaggt
     1501 attccgtcag gcgccctcag cctctcctcg cttctacgcg cgaacaccga tatgcccgat
     1561 ctgcactgga ccattccggt cgctcgctgg tctagctggc ctgctgccgc agctgccgca
     1621 cccgatatcg gctttatcga gccgatcgtg cgtcgtcgcc tgagcacttt gtcccgcgtt
     1681 gccctgaagg tcgcgcacga ctgcgtggcg caagacgaca tccgggttgt gttcgcctcg
     1741 cgccacggcg aattacgccg tacaactgag atcctccacg cgatcagcgc gggcgagccg
     1801 gtctcgccaa cggccttcag tctgtccgtg ctgaacgcaa tgaccggcgt gttcggcatc
     1861 gcgcgcggcg atcgctccgc tgccagcgcg atctcggccg gtgccgagac gctcggctac
     1921 gcgctgctgg aggcgtacgc gcaatacacc gcccagcccg gctcgccggt cttgctggtg
     1981 tacgccgacg aaccagccga tccggcatac ggcacgattg aagacgaagt gcagggcggc
     2041 gcgatcgcca tcctgctgga cggcgaagca gcgatggggc aactcgtttg cacgttgtct
     2101 ggcgcaggtg agccggagcc agcgaagacc gctgaggagg cgggtcacgc agctgaggca
     2161 ggtcacgcag atgaggcaag ccacgcagct gaggcaagcc acgcaggcaa ggccagccaa
     2221 gcaggcaagc caacccacgc aatcgagcca ggcgcagcgc tcccggccga aagcttcgcg
     2281 acccagacgc aggcgctgct gcattgtctg gaaacgggca agcccgccgt ctggccaggt
     2341 gccggcgccg cctggcattg gaggtggcat gaacgcgcgc cttgattacc tttggcgatt
     2401 cttcgcgacg ggcatggcct tcgtggtgtt cggcatctgc ggtctgctgt tttcggtttt
     2461 ggtgttcccg ctcgcgtggc tgtggccgca tcgtgcctcg cgtcagcttg cggtgacgac
     2521 cgtgatccac tggtttttcc gtgccctggt cgcggtgttg cagcgcatcg gcgtgatgga
     2581 gctcgaggtg tcgggcgcag aggcattgcg cgccggcggc ccggccatcg tggtcgccaa
     2641 ccacccgacg tacctggacg tgatggtgct gttgtcgctt acgccgcgtg cctgttgtgt
     2701 ggtgaaaaac gcgcactggg gtaatccctg tttctgggga atcgtgcgtt cggcggaata
     2761 tgtgagcaat gccgatccct cggaactggt cgaggcgggc gcgcggcaac tcgcagcggg
     2821 ctacacgatg attatttttc ccgaaggcac gcgcagcccg gcgccgaacc ggctgcacgc
     2881 attttcacgc ggtttcgcgc acatggcgtt gaaggtcggc gcgccgatcg tgccggtgct
     2941 gatggattgc gatccgccgg cgttcaccaa gcagatgcgc tggtacgacg tgccggcgcg
     3001 cgcttttcgc atgcgtgtga acgtgctcgc gccgtttggc gtcgagcggc ttgcggacca
     3061 cgatccttca ccgtctctcg cggcgcgcag cgtgaccagc gccatcgaag cccacattac
     3121 tcagcacctg ttcgactatg gattctttaa aactggaaat taagcagctc cttatcgaag
     3181 ccctcgatct ggaagacctg agcccggccg atattgacga cgacgctccg ttgttcgata
     3241 ccgacggcat cggtctcgac tcgatcgacg cgctcgagat cggcatcgtg ctgcgcaaac
     3301 aatatcaact gacgattgca gcgaacgacg aacgcacccg cgaccacttc cgctcgatca
     3361 gcacgctggc cgcgctggta gcgagtcaac gcgaagcggt gcatgctgtg aacgaaacca
     3421 caaaaaaagg ggattaatcg tgtccgaggc agagattctt gaacgcatcc gcgccatctt
     3481 caaagagaac ttcgcgatcg agcccgaacg tgtgacgccc gacgcgcatc tcttcgaaga
     3541 cctcgatctc gacagcatcg acgccgtcga cctcgcgatc aaactgcagg aaatgaccgg
     3601 ccgccgtatc aagccggaag aattcaagtc ggtgcgcacg gtcggcgacg tgatcggtgc
     3661 ggttgagtcc ctgctggcgg cacaaggctg atgccatccg tgcgctcgcg aatgcaagcg
     3721 acggcgagcc agcccggtga ggcacagcct ggcactgaac gccactgggc caaaacggta
     3781 gtgcaggtcc tgttgaaact cgcgtacccc gcgttgattc tgtgtgcctg gcgctgggac
     3841 acgccgcgct acgtcggctg tatgctgttt gtcattttgt ggctgcaacg ttgggccggc
     3901 agcggtccag tcgcgacttc gctgcgccgg ctctccacga tcgactggac cgtggtcggc
     3961 ttgctgagct gcgcttcggc ggccatcgtg ttcaccaaca gcgagttgct gttgcgcctc
     4021 tatccgtcgc tcgtcaatct gggtctgctg atcgcgttcg gcgcgacgct cgggcgcggg
     4081 ccgtcgatga tcgaaaaatt cgcccggctg ggtaatccga atctgtcgcc gggcgccgtg
     4141 cgccatacgc ggcgtgtgac ccaggtgtgg tgcgtattct ttgcgctgaa cggcgcgttc
     4201 tctgcttaca cggcgctgta ctggagccgt gcaagctggt cgctctataa cggcgcgatc
     4261 gcgtatgggc tgatcggcgt gttgctggtg gccgagatca tctggcgtta tctggtggtg
     4321 ctgccgcgag ccgcgcgttc ggaggcggca tgatcgcgtt gcatgagctg ttgttggcgg
     4381 accgtgaggc ctccgccatg aatgcaccgg tatgccgcga tggcgcgacg gtactcgatc
     4441 gcgctgcgtt tcgcgcgcgc gtctcggctt tggtcgcgtt gatgcaaacg caagacgcgc
     4501 aacgttacgc gttatgtatc gacgatccgt tcgatttcgc ctgcgcgctg tttgcgttgt
     4561 tcgcgtgcgg caaggagccg gtaattccgg ccaatgcgac gccgggctat ctcgccgatc
     4621 ttgccgatgc gtacgacgcc gtgctgaccg acgccgacct ggcgcatggc gtgcgcgacg
     4681 ccggcgcgga tgctgacgac aaacaggaca aacgtgacaa acaggacaaa cccgacaatc
     4741 ccgcgcgagg cacctcccac acgatcgatc cgcaagcccc gttgacgctc tacacgtcgg
     4801 gcagcagcgg cacacccaag ccgatccgca agactctcgc gcaattcaat gccgaagtgc
     4861 acacgctcga aaagcagtgg ggcgcgctca tcggcgacgc gacgatgctc gcgagcgtgc
     4921 cgcatcatca catctacggt ttgctgtttc gcgtgttgtg gccgcttgcc gcgggccgtg
     4981 cgttcgagcg cgcgatcagc atcgagcctt tgcatttgca agcgcagata caaacacaga
     5041 ttcaagcgca gatcgcgcca tgcggcgcca cggtggttgt ctccacaccc gcgcagttgt
     5101 cgcgttggcc cgcgttgcct ggcttcgccg cgttgacgcc ggcgccgcgc gcattctttt
     5161 cttcaggcgg cccactcgcc gtggaagccg cgcaagaata cgcaacggcc tacggcgccg
     5221 cgccgatcga aatctacggc agtacggaaa ccggcgggat cgcgtggcgg cgtcaggatc
     5281 agaccgatgc ctggcagtcc gtgaccggca tcgagatacg ctgcgacgaa gacggcgcgt
     5341 taaacgttcg ctcgccgcat ctcgatcacg acggctggca tcgtactgac gacaaaatcg
     5401 cggtcgatgc cgacggccgc ttccgcctgc aaggccgtct cgatcgcgtg ctcaagctgg
     5461 acggcaagcg cgtatcgttg ccggagctcg aagcgcggct ggcgttgcat ccgtacgtcg
     5521 cccaggcggc gatcgtgccg ctggcaggcg cctcgcgcga gcgcgtcggc gccgtggtgg
     5581 cgctgaccga agcgggcagc gaggcattgc gcggcgaagg ccgcgtgctg ctcgcgaaaa
     5641 ccctgcgccg gcatctcgcc gcgtatttcg acctggtcgt gctgccgcgt cattggcgtt
     5701 ttcgcctcac gttgccgttc gacgcgcgcg gcaaactgcc ggtggccgct gttgcggcgg
     5761 ccttcgaggc acgcgcggac ggtgtggaag tgctcgccga agcccgcagc gccgacacgc
     5821 tgcactacga actgcgcgtg ccgccggcgc tcgtgcattt cgccggccac ttccccggtc
     5881 tgccgattct gccgggtgtc gtccaggtgc actgggcgat gcatctggcc accgaacacg
     5941 tgcccgccgt gcgcgcgctg gcatcgatcg accggctcaa gttcatggcg ccggtgccgc
     6001 cgggcgcggt gctgaatctc acgctcgccc acgacgccga gcgtcgtcgc gtgcagttcg
     6061 cgtaccgctt gagcggccgt gaatgcgcgt cgggcgtgat cgtgtatcgg gagcctgcgt
     6121 gatgagcttc gccccgtgca tcgtcattcc gatttacaac cataaggacg cgattggctc
     6181 gaccgtcgcc catctcgcgg tgcacggctt gccgatcttt gtcgtcgacg acggcagcga
     6241 tgaagcgacc cagcaggtgc tcgccgggtt ggcgcggcag tacgccgggc agctcacgct
     6301 gctgcggttg ccggtcaacg
//
LOCUS       DATASH010000075        15719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5152131,
            whole genome shotgun sequence.
ACCESSION   DATASH010000075 DATASH010000000
VERSION     DATASH010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 15719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15719
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5152131"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="VHV99_02170"
     CDS             <1..551
                     /locus_tag="VHV99_02170"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEX3634294.1"
                     /translation="LRADGLIDAKHGIGYFVAQKSASDYGFPTSAKHLGFSSSMLDLL
                     ELRMAVEIHAAGLAAARRSWAQEAKIFANAENFATALERGESPDLADWAFHRSISEAT
                     NNAAFVEFFDRMGLSVLPRRAIEGEKRDALITRQYLEKSIPEHRAVCEAISRGDVEAA
                     REAMKGHLGGSQARYRGLVGTE"
     gene            complement(605..1444)
                     /locus_tag="VHV99_02175"
     CDS             complement(605..1444)
                     /locus_tag="VHV99_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX3634295.1"
                     /translation="MKKAQALDAIGEGRLTPERERRQRVQSASTAMAVLKGLAAIGGR
                     ASLTALASHIGESPAKVHRYLVSLMEESLVAQESGTQQYVLGTECLAIGLAAMRLADP
                     IRLAEPSLVHLRESLEITCFIAVMGNRGPTIVRFEEPGLPVTVNVRAGSVMSLLWSAT
                     GRVFLGLLDESRVRALAEEELLHSSADLLALLDRNDPIGQIMRDVQAVQCATVKDTNL
                     KGISAVAAPLFDFNGRLCATLTALGATGGFDSSIDGAIAEAVRQEAQAISARLGYVAQ
                     TQA"
     gene            1564..3909
                     /locus_tag="VHV99_02180"
     CDS             1564..3909
                     /locus_tag="VHV99_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016348863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450/oxidoreductase"
                     /protein_id="HEX3634296.1"
                     /translation="MSDAIDPDRASACPFHGSAGGSLPATPAGCPVSRRAAEFDPFED
                     GYQQDPPEYVRWAREQEPVFYSPKLGYWVVTRYEDIKAIFRDNLTFSPSIALEKITPT
                     GPEANEVLASYGYAMNRTLVNEDEPAHMPRRRALMDPFTPAALAHHEPMVRRLAREYV
                     DRFIDDGKADLVDQMLWEVPLTVALHFLGVPEEDMDTLRKYSIAHTVNTWGRPRPEEQ
                     VEVAHAVGKFWQFAGKVLDKMRQDPDGSGWMQYGIRKQKELPEVVTDSYLHSMMMAGI
                     VAAHETTANAAANAMKLLLSHPEAWRDICDDPGLIPNAVEECLRHNGSVAAWRRLATR
                     DVTVGGVDMPAGSKLLIVTSSANHDEQQFADADLFDIRRENASDQLTFGYGSHQCMGK
                     NLARMEMQIFLEEFARRLPHMRLAEQRFTYVPNTSFRGPEHLLVEWDPALNPERSDAS
                     VLQPRAAVRVGEPSAHSLSRPVVVTSVSFAGTDIVHVKLASPDGRALPRWTPGSHIDV
                     ECGNTGISRQYSLCGDPDNAGTFEIAVLREPRGRGGSEWLHNTVKAGALLRIRGPRNH
                     FRLDETAKKVVLIAGGIGITPISAMARRAKVLGLDYVVHFSGRTRASMALLGELTALH
                     GERMQVYVSDEGRRNDFSALPVDEGTQVYACGPARMLDALQQASASWPDDALKVEHFE
                     STAGTLDPDKEHPFEVELKDSGLVVMVPADQTLLVALRQANVDVQSDCEEGLCGSCEV
                     RVLAGEVDHRDVVLTKGERQLNTRMMTCCSRAKGTKLVLEL"
     gene            complement(4036..4908)
                     /locus_tag="VHV99_02185"
     CDS             complement(4036..4908)
                     /locus_tag="VHV99_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3634297.1"
                     /translation="MSTLRFLRTFVAVARYGSFAVAAEQMALTQSAVSMQMRALETEF
                     KHELFDRCGRKVMLNAMGKSLLPHAQQLLSLYEAMRMAAGGLEEDVGPVSIGAVESVV
                     GALAEAAAHLKIARPNLDVRIMAAKSVDLAARVDSGEIDAAVIIDTLGRRPASVQWTP
                     VYSEPLVLLANATMVPESVPELLKSQRFLRFDRTQRTGIVIDRALRKQRLKVNEFLEL
                     NSLEGIAELVRQGIGVAVVPLLRRSSWLCDDALRVMPLPQMDARRIVGMLERAEHGKM
                     AVTAAIVRHLKEVI"
     gene            5252..6445
                     /locus_tag="VHV99_02190"
     CDS             5252..6445
                     /locus_tag="VHV99_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HEX3634298.1"
                     /translation="MKIIPEITQAQAEIQAIRRDIHAHPELCYEENRTADVVAQKLAS
                     WGIEVTRGLGKTGVVGVLRNGSSRKAIGLRADMDALPIQELNTFAHASQHTGKMHACG
                     HDGHTAMLLGAAQYLSQHRNFDGTVVFIFQPAEEGGGGAKAMIEDGLFERFPVDAVFA
                     LHNWPGMAAGEFGARVGATQASSNEFRIQVKGVGAHAAIPHNGIDPVFTAMQIGTGLQ
                     SIMTRNKRPIDAAVLSITQINAGEAVNVIPDTATLAGTVRTFSVDVLDLIETRMKQLA
                     EATALAYGCSVEFSFRRNYPPTVNTEKETHFALGVMQDIVGKDRVETNIDPTMGAEDF
                     SFMLLEKPGCYAYIGNGHGDHRDHGHGLGPCMLHNTSYDFNDDVLSLGSTYWVRLTES
                     FLTPA"
     gene            6488..7597
                     /locus_tag="VHV99_02195"
     CDS             6488..7597
                     /locus_tag="VHV99_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HEX3634299.1"
                     /translation="MNESQYFQRSYANARERYRRAAAAIQARVEPHVIPGHQGLEGED
                     LSTDVVRLGPAMARRMLVLTSGTHGVEGFCGSAAQIALLHDRPLQALLDKLNVAILVV
                     HAINPYGFSHLSRTNEDNVDLNRNSIDFSLPLPVNPAYDDLHALLVPSSWPPSDENAA
                     AIADYISQRGEQAYQQALTVGQYQHADGMFFGGHRSVWSTRVLQMVFEQHGAACDSIG
                     WIDFHTGLGPYGHGEKICVGQLGNGELQRARDWWGADVTSPLDGTSVAANVAGPVLDT
                     LRRTCTHADTTAIAIEYGTIPLVDMLHMLRADVWLRQHPQAEKQQISAIREQIRAAFY
                     CDDDVWRGMILGQARAAILQAVMGLSRESASVGSK"
     gene            7754..8164
                     /locus_tag="VHV99_02200"
     CDS             7754..8164
                     /locus_tag="VHV99_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX3634300.1"
                     /translation="MTPVSAVPAADSAAAYAHFSASLSFETDCADVHAALASDDPRSV
                     DFVLLDVRGPAHFSRGHLPGAVNLPHGKIVASKLAEYPSDTLFITYCAGPHCNGATRA
                     AMRLAQLGRPVKVMIGGITGWLDEGFQLAIADEG"
     gene            8352..9329
                     /gene="ftrA"
                     /locus_tag="VHV99_02205"
     CDS             8352..9329
                     /gene="ftrA"
                     /locus_tag="VHV99_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FtrA"
                     /protein_id="HEX3634301.1"
                     /translation="MHNHLVVALAYDRLCTFEFGCVTELFALERPELGVNWYRFAVCA
                     SEPGPVRAAGGITVAAPYTLKLLDRADTIVIPGWRDADELPPEPLLKKIRAAYARGAR
                     LCSICSGVFVLAASGVLDGKTVTTHWRYADKLQQRYPHLHVQPDALYVDENQIITSAG
                     SAAGLDMLLHLVRRDHGSAVANRVAQRLVVAPHREGGQAQFVPRPMPQDGGGRLAKLM
                     DWVRRHPALPHTLRSLAEWAAMSPRTLQRQFHDATGMAPYEWLVRERVAIARELLEAP
                     SALPMSRVAELAGFGSEESLRRHFRRIALTSPGAYRKRFGVQAGVQTAG"
     gene            9697..10638
                     /locus_tag="VHV99_02210"
     CDS             9697..10638
                     /locus_tag="VHV99_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634302.1"
                     /translation="MNVKTAVAAAAVVVTVVAALMPALAASQTASTDSRADLYRRNLL
                     TGKDVPCRTNASCAALGVAALEAGRIKEARTLVAMEAALAEATAMQAGEQNSPKATSS
                     ARARVAMALVHQGDVQLKLGAFPNARAYYRTAVSRGNDYPNDALLGRAVAAARQRLEA
                     IAGKAVVNGVPPNGARFASYMFFGAWNSIEVKPVKGRHGVYRIDGDFVYPSVGPDGQP
                     GANMGSLSAYVRFYDGVARVPMTDEGGSAPLDATARITHLTPYEKHDGKHADKCLIEF
                     RLSAPETLDVAAHGSPTGCGFGFNVSADGRYYLMTGS"
     gene            10706..11923
                     /locus_tag="VHV99_02215"
     CDS             10706..11923
                     /locus_tag="VHV99_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HEX3634303.1"
                     /translation="MSDIAKIIAGFNAGRDPERLAMKYKLMRGSPFVFLRGTCHLFYT
                     RLPHDKVFDEAPPVWICGDMHLENFGSYKADNRLIYFDNNDFDEACLAPNLYELVRLL
                     TSVLVGASDLKLSRAQALALCHTAVDAYGAALAFGKSRWIEPETSTGMVRDLFDALAS
                     RSRSAHLDRRTVLKGKTRVLKVDGKKALPVSDKARAAVTDFMNKFAVGEPNPDFYRII
                     DVARRIAGTGSLGVDRYVILVEGKGSPDGNYLIDLKEALPSSVTPHVRTVQPKWQSEA
                     QRVVEIQRRNQAVSQAFLHAVDFNQRSYVLRSLQPSEDRVALSDWDGKLPRLEEVVNS
                     MAELSAWGQLRSGGRQKSSIADELIAFGNRRDWQLPLIELATQCEAQVIADWKVYCEA
                     YDGGAFNLVVKGA"
     gene            12192..14621
                     /locus_tag="VHV99_02220"
     CDS             12192..14621
                     /locus_tag="VHV99_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HEX3634304.1"
                     /translation="MKKKRFDEGHTGVAARLLAAFVLLVVAFGANADDYCYSLYAKSG
                     ATPGTPSCRYDVAGNTPGGMGNYACINNISLIDQWCATPATDEPEQSCPVADPVYPGS
                     GAVTLTEADFVSGDDLPMQFKRTYRSKSLARNVAAMGPVWFHSWQRNLGLANANSGSS
                     SKVLAYRENGEPITFNWSAGTWRAVPGSYLTLSQSGSGWTLTDLKTDTVESYSAQGTL
                     LSESTKTGFVRALGYDGSGLLTTITQHAAGTDASKDITLRLDYDEKRRLSRLNDPLGG
                     MTQYGYDANGNLVLVTWPDGNAYRYVYDDSRFKNALTGELDEVGNRIATWSYDSQGRA
                     TGVSHPDSTRNAQFSYGAGTTSVTDSKRTTTLSFSSVGGMVRPTSSSSVDGTANLAWD
                     ALGNLLKDSGAGGNTEYVYDEVGRPVRITGNSASGTSITTIRYADATSLHPSIIAAPG
                     VIQSVVYDARGNATGMSETPTTDSTGASGFDAVKSDGVTRAYGMVYDAFNRLTFVQMY
                     EGSALVSQWVVTHDATGNVFSIYPTDEYVASETIARDASHRPLRGFNPTGDFNLRYDR
                     RGRIDLFKFNEYASPANGGVRRVFKVRFGYSPDGQVVSRTGTVAKNGSLLDLNDGTDI
                     AISSGEIDQWIDNYNLGESPVGPPANLQGARRLLGDNFLGTSTVCSGCHFSAGLPDGI
                     LRGIVFVWRLVQNPAVRYGIGQGVQKARQTIERIKEQCKPVVKDTSEINWGQNPNQLY
                     HTFRHLEEQGIPQQPVMNAIREDLAERGSTIQLGDNATTMTVTVDGRAIDYIAFRQSS
                     NTIRVGSIRPK"
     gene            14633..15058
                     /locus_tag="VHV99_02225"
     CDS             14633..15058
                     /locus_tag="VHV99_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634305.1"
                     /translation="MSRPLSAKEHALLKFLIEANEPLYGDRTNQWKTQVETCLVREID
                     SPYFLAVIHEESIEKAGCDSITLGYELVGVDHGVPVLIYAVAMKTPSDYFIDIFNVDR
                     LDGEPLVNYPEAGDGLMIVERNKRVGGADLRHLYGGSRS"
     gene            15349..>15719
                     /locus_tag="VHV99_02230"
     CDS             15349..>15719
                     /locus_tag="VHV99_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634306.1"
                     /translation="MQRTFFKPQERRLLEFLLKVNEPFYGAYVSRWTAQLETCTVREV
                     NVPYCLAISHDETRLSGGGFTTLARELVCLDEDVPVLIYACAVETQSGYVLDSFDIDR
                     LDGEPLAVYPEPGDGLMIMEAG"
BASE COUNT         3101 a         4644 c         4891 g         3083 t
ORIGIN      
        1 cgttgcgggc tgatggactg atcgatgcca agcatggcat cggctatttt gtcgcgcaga
       61 agtccgccag cgactatggc tttccaacca gcgcgaagca ccttggcttt tcctcatcca
      121 tgctcgatct gctcgagtta cgcatggctg tggaaatcca cgcggcgggc cttgcggctg
      181 cgcgccgatc gtgggcgcaa gaagccaaga tcttcgccaa cgcggaaaac tttgcgacgg
      241 cgttggaaag gggcgagtcg ccggatttag cggattgggc gtttcatcgc tctatctcgg
      301 aggcgacgaa caatgcggca ttcgtcgagt tctttgatcg gatggggcta tcggttcttc
      361 cgcggcgcgc gatcgaggga gaaaaacgcg atgcgctgat caccaggcaa tatctggaaa
      421 aatccattcc cgagcaccgt gcggtctgcg aggccatcag ccgaggcgac gtcgaagcgg
      481 cgcgcgaagc gatgaagggc caccttggcg gcagtcaggc ccgctaccga ggcctcgtgg
      541 gaacagaata ggaccccggc aggctgaacg gttcgctatt tctcctgctg gcgacgcaaa
      601 ggcgtcaggc ctgcgtttgc gctacatatc cgagccgggc gctgatagcc tgcgcctcct
      661 gtcgaactgc ctccgcaatc gctccatcga tagacgaatc gaatcctccc gttgcaccca
      721 gcgccgtgag tgtggcgcac agccggccgt tgaaatcgaa aagcggtgcg gccaccgcgc
      781 tgatgccttt caggttggtg tccttcacgg ttgcgcactg aacggcctgc acgtcccgca
      841 ttatctgtcc gatgggatcg ttgcggtcga gcagggcgag aaggtcggcg gacgaatgca
      901 gcaactcttc ttccgccagg gcccgaacgc gggattcgtc gagcaggccc agaaataccc
      961 ggcccgtcgc tgaccatagc agcgacatga ccgatccggc gcgcacgttc accgtgacgg
     1021 gcagccccgg ctcctcgaag cgcacgatcg tcggcccgcg attgcccatc accgcaatga
     1081 agcaggtgat ttcaaggctt tctctcagat gcaccagcga cggctcggca agccggatcg
     1141 ggtcggcgag ccgcatcgcg gcgaggccga tggcaagaca ctcggttccc aatacgtatt
     1201 gctgggtgcc cgattcttgc gctaccagtg attcttccat caggctcacc agatagcggt
     1261 gaaccttcgc cggactttcg ccgatgtgcg atgccagcgc ggtaagcgac gcgcgtccgc
     1321 cgatcgccgc gagccccttg agtacggcca tggcggtgga ggcggactgg acgcgctggc
     1381 ggcgctcgcg ttctggtgtg aggcgtcctt ccccgatcgc gtcaagtgct tgagcttttt
     1441 tcatgcgtcc attctagcgg gcggctggtg acttccatcg gcgcgcgcgc gtaaaccctg
     1501 atgagttaat tacgcatagc gtactatagt tacgcataat cgatatgcag caaggaggca
     1561 tgaatgagcg acgctattga cccggatcgc gcttcggcgt gccccttcca cggcagcgcg
     1621 ggcggcagcc ttccggcgac acccgcaggt tgcccggtca gccgccgcgc cgcggaattc
     1681 gatccgttcg aagacggcta ccaacaggat ccgccggaat acgtgcgctg ggcgcgcgaa
     1741 caggaaccgg ttttctacag cccgaaactc ggctactggg tcgtgacgcg ctacgaagac
     1801 atcaaggcga tctttcgcga caacctcacc ttcagcccgt caatcgccct cgagaaaatc
     1861 acgccgaccg gacccgaagc gaatgaggtg ctggcgtcat acgggtatgc catgaaccgg
     1921 acgctagtca acgaggacga accggcacat atgccgcgcc ggcgcgcgct gatggatccg
     1981 ttcacgcctg cggcgctcgc gcaccacgaa ccgatggtgc gccgtctcgc gcgcgagtac
     2041 gtcgaccgtt ttatcgacga cggcaaggcc gacctcgtcg atcagatgct ctgggaggtg
     2101 ccgctcaccg ttgcgctgca tttcctgggc gtgccggaag aagacatgga cacgctgcgc
     2161 aagtattcga ttgcgcacac ggtcaatacg tggggtcggc ccaggcctga agagcaggtc
     2221 gaagtcgcgc acgcggtcgg caagttctgg cagtttgccg gcaaggtgct ggacaagatg
     2281 cgtcaggacc cggacggttc aggctggatg cagtacggca ttcgcaagca gaaggaactg
     2341 cccgaggtcg tgaccgattc gtatctgcat tcgatgatga tggcgggcat cgtggccgcc
     2401 cacgaaacca ccgcgaacgc cgccgcgaac gcgatgaagc tgctgctttc gcatcccgag
     2461 gcgtggcgcg atatctgcga cgacccgggc ctgataccga atgccgtgga ggagtgcctg
     2521 cggcacaacg gctcggtggc cgcatggcgg cgcctcgcga cccgggacgt gacggtcggt
     2581 ggcgtcgaca tgccggcagg atcgaagctg ctgatcgtga cgtcgtccgc gaatcacgac
     2641 gaacagcagt tcgccgacgc cgatctgttc gacatccggc gcgagaatgc aagcgatcaa
     2701 ctgacgttcg gctatggatc gcatcagtgc atgggcaaga atctcgcgcg tatggaaatg
     2761 cagattttcc tggaagagtt cgcgcgtcgg ctaccgcaca tgcgccttgc cgaacagcgc
     2821 ttcacgtatg ttcccaacac gtcgtttcgt ggccctgagc atctgctggt cgaatgggac
     2881 ccggcgctca atcccgaacg ctccgatgcg tcggtgttgc aaccccgcgc cgcggtgcgc
     2941 gtcggagagc cgtctgcgca ttcgctcagc cgtcccgttg tcgtcacgtc ggtgagcttc
     3001 gccggcacgg atatcgttca cgtcaagctc gcgtcacctg acggtcgagc gctgcctcga
     3061 tggacgccgg gttcgcatat cgacgtcgaa tgcggcaaca cgggcatctc gcggcagtac
     3121 tcgctgtgcg gcgaccctga caatgccggc accttcgaga tcgcggtgct cagagagccc
     3181 cgggggaggg gcggttccga gtggctgcat aacactgtca aggctggcgc attgcttcgc
     3241 atacgcggcc cacgcaatca tttccggctc gacgagacag cgaagaaggt cgtcctgatc
     3301 gcgggtggaa tcggcattac gccgatcagc gcgatggccc ggcgtgcgaa ggtgttgggt
     3361 ctcgactatg tcgtgcattt cagtggccgc acgcgcgcct cgatggcgct gctcggcgaa
     3421 ctcacggcgc ttcacggcga gcgtatgcag gtgtatgtct ctgacgaagg caggcgcaat
     3481 gacttcagcg cattaccggt cgacgaaggc acccaggtgt atgcatgcgg accggcgcgc
     3541 atgctcgacg cgctgcaaca agcatccgct agctggcccg atgacgcgct gaaggtcgag
     3601 catttcgaat cgacggccgg cacactcgat ccggacaagg aacacccctt cgaagtcgag
     3661 ttgaaggatt cgggacttgt cgtgatggtg cccgccgatc aaacactgct ggtcgccctg
     3721 cgccaggcga acgtcgacgt tcagagcgat tgcgaggaag gactgtgcgg ctcgtgcgag
     3781 gtgcgcgtgc tggcgggcga agtcgatcat cgcgatgtcg tgctgaccaa aggcgagcgg
     3841 caattgaata cccggatgat gacctgctgc tcgcgtgcga aaggcacgaa gctcgtgctg
     3901 gaactctgaa gcgggggacg cttcgccgga agcgtccccg cgttattcgc gccagtgttt
     3961 cgtctatggc gagcgcgccc ggatggggcg caaacgggcg cagtgcggaa cagggcggca
     4021 actccctgac gatccttaaa tcacctcctt caggtgcctc acaatcgccg ctgtcacggc
     4081 catcttgccg tgctccgcgc gctccagcat gcccacgatc cgcctcgcat ccatctgggg
     4141 cagcggcatc acccgcaacg cgtcatcgca cagccacgac gagcgccgca gcagcggcac
     4201 aaccgcgacg ccgatgcctt ggcgcaccag ttccgcaatg ccttccagcg agttgagttc
     4261 gagaaattcg ttcaccttca ggcgctgctt gcgtagcgcg cgatcaatga cgatgccggt
     4321 tcgctgcgtc cggtcaaagc gcaggaaccg ctgcgacttg agcaactcgg gtaccgattc
     4381 tggaaccatg gtcgcattgg cgagcagcac gagcggttcc gaatagacag gtgtccattg
     4441 cacgctggct ggccggcgcc caagggtgtc gatgatcacg gccgcatcga tctcgcccga
     4501 gtcgacgcgc gcggccaggt cgacggattt tgccgccatg atcctcacat cgagattggg
     4561 gcgcgcgatc ttcagatgcg ctgccgcctc ggcgagcgcg cccacgaccg actcgaccgc
     4621 cccgatggaa accggcccga cgtcttcttc gaggccgccc gccgccatcc tcatggcctc
     4681 atacagcgag agcaactgct gcgcgtgcgg caacaggctt ttgcccatcg cattcagcat
     4741 gaccttgcgc ccgcagcggt cgaacagttc atgtttgaac tcggtttcga gtgcgcgcat
     4801 ctgcatgctg accgccgatt gcgtgagcgc catctgctcg gcggcaaccg caaacgagcc
     4861 gtagcgtgcc acggccacga aggtacggag aaagcgtaac gtgctcaagt caacctcgac
     4921 gacgccggag gcgaatgcgt ccgcaactgg ccggacacga ttaatacatc aaaaattttg
     4981 aagcgaatga caacaaattt tagctttctt tgatgataac ggctatggac aatgagtagt
     5041 cgaaagacga accggtatcg ggcacaaggc atgaaggtga cccaactgcg tttcagtgct
     5101 gaacgggccg cttgattgat ccggaacaga ctatttgcca tgctcaaggg agacgtcgga
     5161 ttcgtcacga ttgcactgct gaaggaccgc gcgaagcagc ccctgatgta accgctcaaa
     5221 cgaaccactt cttaagaaag caggaaccct tatgaaaatc attcccgaga ttacccaggc
     5281 tcaagctgag atccaggcca ttcgtcgcga cattcacgcg catccggaac tctgctacga
     5341 agagaaccgc accgcggacg tcgtcgctca gaagctcgcg tcctggggaa tcgaagtcac
     5401 gcgcggactg ggcaagacgg gcgtcgtcgg cgtgctgcgt aacggctcca gccgcaaggc
     5461 gatcggcttg cgtgccgaca tggacgcact tccgatccag gaactcaaca cgttcgcgca
     5521 tgcgtcccag cataccggca agatgcatgc gtgcggccac gatggccata ccgccatgct
     5581 gctcggcgcg gcgcagtatc tttcccaaca ccggaatttc gatggcaccg tcgtgttcat
     5641 ctttcagcct gccgaggagg gcggcggcgg cgcaaaggcc atgatcgaag acggactgtt
     5701 cgaacgcttt ccggtcgatg ctgtgttcgc gcttcacaac tggccgggta tggctgccgg
     5761 cgaattcggc gcgcgggtcg gcgcgacgca ggcgtcgagc aacgaattcc gcattcaggt
     5821 gaagggtgtc ggcgcgcacg ctgccattcc gcacaatggc atcgatccgg tgttcaccgc
     5881 gatgcagatc ggcacgggcc tgcagagcat catgacgcgc aataagcgtc cgatcgacgc
     5941 ggcggtcctg tcgatcacgc agatcaatgc gggcgaagcc gtcaacgtga ttcccgatac
     6001 ggcgacgctt gccggcacgg tgcgcacgtt ctcggtggac gtgctcgacc tgatcgaaac
     6061 ccgcatgaag cagctcgccg aagcgacggc gctcgcctat ggctgcagcg tcgagttctc
     6121 gttcaggcgc aactatcctc cgaccgtcaa tacggagaag gaaacgcatt tcgcactggg
     6181 tgtgatgcag gatatcgtgg gcaaggatcg agtggagacg aacatcgatc cgaccatggg
     6241 cgccgaagac ttctcgttca tgttgctcga gaaaccgggc tgctacgcgt acatcggcaa
     6301 tggtcacggc gatcatcggg accacggtca tggtctcggc ccctgcatgc tgcacaacac
     6361 gagctacgac ttcaacgacg acgtcctgtc gctaggttcg acctactggg tgcgcctcac
     6421 cgaatcgttc ctgacgcccg cgtaagtgtc aggcggggcc cagacacacg tctcacgagg
     6481 agaaccgatg aacgaatcac agtatttcca gcggtcttat gcgaacgccc gcgagcgtta
     6541 caggcgggcc gccgcggcta ttcaggcgcg cgtggaacca cacgtgatac ccgggcacca
     6601 gggcctcgaa ggcgaagacc tgtcgacgga tgtcgtgcga ctcggccccg ccatggcgcg
     6661 gcggatgctc gtgctgacgt ccggcacaca cggggtcgag ggcttctgcg gctcggcggc
     6721 gcagatcgcg ttgttgcacg accggccgct gcaggcgtta ctcgacaaac tgaatgtcgc
     6781 tatcctggtg gtccacgcga tcaatccgta cggcttctcg catttgagcc gcacgaacga
     6841 ggacaacgtc gatctgaatc gcaacagcat cgatttctct ctgccgctgc cggtcaatcc
     6901 cgcctatgac gatctgcacg cattgctggt gccgtcgagc tggccgccgt cggacgaaaa
     6961 cgcggcggcc attgcggact acatctcaca acgcggtgaa caggcctatc agcaggctct
     7021 gacagtgggg caatatcagc atgccgacgg catgttcttc ggcggccatc gttctgtctg
     7081 gagcacgcgg gtattgcaga tggtgttcga gcagcatggc gcagcgtgcg acagcattgg
     7141 ctggatcgat tttcatacgg gactcggccc gtacgggcac ggcgagaaaa tctgcgtagg
     7201 gcagctcggt aacggcgagc tgcagcgcgc ccgcgactgg tggggtgccg acgtgacgag
     7261 tccgctggac ggcacgtcgg ttgcggcaaa cgtagcggga cccgtgctcg acacgctgcg
     7321 ccggacctgc acgcatgccg acaccacggc gatcgcgatc gaatacggca cgattccact
     7381 cgtcgacatg ttgcacatgc tgcgtgccga tgtctggctg cgccagcatc cccaggcgga
     7441 gaagcagcag atcagcgcga tccgcgagca gattcgcgca gccttctact gcgatgacga
     7501 cgtatggcgg gggatgattc tcggacaggc gcgcgcggcg attctgcaag ctgtcatggg
     7561 gctgtcgcgc gaatcggcga gcgtcgggtc aaagtgaccg aggtatcgag atgcgcgcgg
     7621 gagcgcggta acgagtttta acgcggatag cgccatggcg tgatgttact gaaatctgtc
     7681 atttaggccg ctggctcaca gtgcgtgaga acgggacaat gtgtgacaac gattcacaca
     7741 taaggagcct tgaatgaccc cggtctcagc tgttcccgct gccgatagcg ccgccgcgta
     7801 tgcccacttc agcgcgtcgc tgtcattcga aaccgattgc gccgacgtcc acgcggcact
     7861 cgcgagcgac gacccgcgtt cggtcgattt cgtactgctc gacgtgcgcg gtcccgcgca
     7921 tttctcgcgc ggccatctgc caggcgcagt gaatcttccg cacggcaaga tcgtggcatc
     7981 gaagctggcc gagtatccat ccgatacgct cttcatcacc tattgtgcgg gccctcactg
     8041 caacggagca acgcgcgccg caatgaggct tgcccagctt ggccgccctg tcaaggtaat
     8101 gatcggcggc attacaggtt ggctcgacga gggtttccag ctagctattg ccgacgaggg
     8161 ttgagctcgg tgcgatccgc ttgcactcga tgcatggagg cggatccgaa cggcacaccc
     8221 gtgacgggaa caatgatcac atcgcatttt ttctcactgc tattgtgcag gggcagctgg
     8281 agtcttcatt gtcacgacgc cgcacgctcc ggataagtgg cgtgatcggc gatctccggt
     8341 aacatcgcgc catgcacaat catcttgtcg tcgcgctggc ttacgaccgg ctctgtacgt
     8401 tcgaattcgg ctgcgtgacc gaactgttcg cgctcgagcg tcctgaactc ggcgtgaact
     8461 ggtatcgctt cgcggtgtgc gcgagcgaac ccggtccggt tcgcgcagcc ggcggcatca
     8521 ccgtcgccgc gccatacacg ctcaaactgc tggatcgggc tgacacgatc gtcattcccg
     8581 gctggcgcga tgccgacgag ttgccgcccg agccactgct gaagaagatt cgcgccgcct
     8641 acgcgcgcgg cgcgcgcctc tgttcgatct gctccggcgt gttcgtgctg gccgcctcgg
     8701 gcgtgctcga cggcaaaacc gtcactacgc actggcgcta cgccgacaaa ttgcagcagc
     8761 gttatccaca cttgcacgtg cagccggatg cgttgtatgt cgacgaaaac cagatcatca
     8821 cctcggcggg atcggcggca gggctcgata tgctgctgca cctggtgcgg cgcgatcatg
     8881 gcagcgccgt cgccaaccgg gtcgcccagc ggctcgtggt ggcgccgcat cgcgagggcg
     8941 gccaggcgca gttcgtgccg cggcccatgc cgcaagacgg aggcggccgt ctggcgaaac
     9001 tgatggactg ggtgcgccgt catcccgcgt tgccgcacac cttgcgctcg ctggctgaat
     9061 gggcggccat gagtccgcgt acgctgcagc ggcagtttca cgacgccacc ggcatggcgc
     9121 cctacgaatg gctggtgcgc gaacgcgtag cgatcgcgcg agaattgctt gaagcgccga
     9181 gcgcgttacc tatgtcgcgc gtcgcggagt tggcgggctt cggttccgag gagtctctgc
     9241 ggcggcactt ccggcgtatc gccttgacga gcccaggcgc gtatcgtaag aggtttgggg
     9301 tgcaggccgg agtgcagacg gccgggtagg cgcgcgtcac ttttgtcttg ctgtgctcgc
     9361 gagcgtgaac cgttttcaaa cgggcgcttg ccgatgtgca aaatcatgca cgactcatgc
     9421 atgacaagcg gctcgatgca tcccgcatga tgcgtcgaat gtgcttcatg cgagcctccg
     9481 cacatgcgta aaagtcacgg aacaccgtgt gctgggcctt gcgactgaat tgcgcgatca
     9541 gccgcccgcg ttggcggcag gcaaggttcg cgcaatcgcg gattttctgt cagcatcacc
     9601 gccagcgtcc gtggcaatac acgtctatcg tgcggcgcat acggccactt gaagcgcgtc
     9661 ttcgacacgc gtcatcgtca ctgccctctc tttctcatga acgtcaaaac cgccgtcgcc
     9721 gctgctgccg ttgtcgtcac tgttgttgca gcactgatgc ctgcgctcgc cgcgtcgcag
     9781 acggcatcga ccgattcgcg cgccgatctc tatcgacgca acctgctgac gggcaaggac
     9841 gtgccgtgcc gcacgaatgc gtcatgcgcg gcgttgggcg ttgccgcgct cgaagcgggg
     9901 cgcatcaagg aagcgcgaac gctggtcgcg atggaggccg cgctcgccga ggccaccgcc
     9961 atgcaggccg gcgagcagaa ctcgccgaaa gcaaccagtt cggcgcgggc tcgcgtggca
    10021 atggcgctgg tccatcaagg cgacgtgcaa ctgaagctgg gtgcgttccc gaatgcgcgc
    10081 gcgtactacc ggaccgcggt aagccgcggc aatgactatc ccaacgacgc gctgcttgga
    10141 cgtgcggttg cggccgcgcg tcaacgtctc gaagcgatcg ccggcaaggc ggtggtgaac
    10201 ggcgtgccgc cgaacggcgc gcgtttcgcc agttatatgt ttttcggcgc gtggaacagt
    10261 atcgaggtga agccggtaaa gggacggcac ggcgtctatc ggatcgacgg cgacttcgtc
    10321 tatccgagcg tcggccccga cggtcaaccg ggcgcgaaca tgggcagcct gtcggcctat
    10381 gtgcgctttt atgatggcgt ggcgcgcgtg ccgatgaccg atgaaggcgg tagtgcgccg
    10441 ctcgatgcca ccgccaggat tacccacctc acgccttatg aaaaacatga cggcaagcac
    10501 gcggacaaat gcctgatcga gttcaggctc tccgcaccgg agacgctcga cgtagcggca
    10561 catggctcgc cgaccggatg cggtttcggc ttcaacgtca gcgcggacgg gcgttactat
    10621 ctgatgacgg gctcctgacg cggattccat ccactcgcga cgaacccgtc ctgatcccgc
    10681 attgagctgc ttgaagggga aatacatgtc cgatatcgca aagatcatcg ccggtttcaa
    10741 tgcaggccgc gatcctgaac ggctcgcgat gaaatacaag ctcatgcgcg gctcgccgtt
    10801 cgtgttttta cgcggcacct gtcatctgtt ttacacgcgt ctgccgcatg acaaggtatt
    10861 cgacgaagcg ccgccggtgt ggatttgcgg cgatatgcat ctggagaatt tcggtagcta
    10921 caaggctgac aaccgcctga tctatttcga caacaacgat ttcgatgaag cctgtctggc
    10981 gcccaatctt tacgaactgg tgcggcttct gacgagcgtg ctggtcggcg cgagcgatct
    11041 caagctgagc cgcgcgcagg cactcgcgct ttgtcacacc gcggtcgatg cctatggcgc
    11101 ggcgctcgcc ttcggcaaat cacgttggat cgagccggag acgtcgacgg gcatggtgcg
    11161 cgatctgttc gacgcgctgg cgagccgctc gcgctccgcg catctcgatc gccgcaccgt
    11221 gctcaagggc aagacccgcg tgctgaaagt ggacggcaag aaagcgctgc cggtcagcga
    11281 caaggcgcgc gccgctgtca ctgacttcat gaataaattc gccgtggggg aacccaatcc
    11341 cgacttctat cgcatcatcg acgtggcgcg gcgtattgcc ggcaccggca gcctgggcgt
    11401 ggaccgctat gtgattctcg tcgaaggcaa aggctcgccg gacggcaact atctgatcga
    11461 tctgaaagag gcgctgcctt cttcggtcac cccgcatgtg cgcacggtgc agccgaagtg
    11521 gcagagtgag gcgcagcgtg tggttgagat tcagcggcgc aatcaggcgg tttcgcaagc
    11581 gttcctgcat gcggttgatt tcaatcagcg ctcgtatgtg ctgcgcagtt tgcaaccttc
    11641 ggaagatagg gtggcattga gcgattggga cggcaagctg ccgcggctcg aagaagtggt
    11701 caacagcatg gctgagctga gcgcgtgggg gcaattgcgc agcggtgggc ggcagaagtc
    11761 gtcgattgcg gatgagttga ttgcgtttgg taatcgcagg gattggcagt tgccgttgat
    11821 tgagctggcg acgcaatgcg aggcgcaggt gatcgccgac tggaaggtgt attgcgaagc
    11881 ttatgatggc ggcgcgttta atctggtggt gaaaggggct tgatggggcg ctatctactt
    11941 tcgcgcagaa atgtcttgcg atcggactag tcgcgagatt tttcgtcttt cctttcttgt
    12001 tgccgtcgtc gctacgtctc gatggcctac acggtctttc gtgatgtctc acgaattgtt
    12061 ctgaacgtat cggcagcctc cgctgcgagg gcgccaggaa gccgaaactt taggatttgt
    12121 cggattccct cgatggagtc ttccttatag ggtgcttggg cttcaaccaa gtaaaactaa
    12181 taaggaaggc tatgaaaaag aaaagatttg atgagggaca cacgggtgtg gcggcccgcc
    12241 tgcttgccgc attcgtgctg cttgtggtcg cgtttggcgc aaacgcggac gactactgct
    12301 attcgctcta cgcgaaatcc ggcgctacgc ctggtactcc atcgtgccgg tatgacgtag
    12361 cgggtaacac gcccggaggc atggggaact atgcctgcat caataacatt tccctgattg
    12421 atcagtggtg cgcgacgcct gcgacggacg aaccggaaca aagctgcccg gttgctgacc
    12481 ccgtgtatcc cggcagtggc gcagtcacgc taacggaggc tgatttcgtt agcggtgacg
    12541 acctgccgat gcagttcaag cgcacctatc gatcaaagtc gctggccagg aatgttgctg
    12601 cgatggggcc ggtctggttt catagctggc aacgcaacct cggactggcg aacgccaaca
    12661 gtggtagctc gtccaaagtg cttgcgtatc gcgagaacgg tgagccgatc acattcaatt
    12721 ggtctgccgg cacctggcgc gctgtgcctg gaagttacct gacgctgagt caatccggtt
    12781 ccggatggac actcaccgat ctcaaaaccg ataccgttga gtcgtactca gcgcagggga
    12841 cgctgctttc ggaaagcacg aagacgggat ttgtccgcgc actgggctat gacggttcag
    12901 ggctgctgac cacgattacg cagcatgccg caggtactga cgcgagcaag gacattacgc
    12961 ttcgtctcga ctacgacgag aagcggcgtc tttcacgcct gaatgatccg cttggaggca
    13021 tgactcagta cgggtacgac gcgaacggca atcttgtttt ggtcacgtgg ccggatggaa
    13081 atgcatatcg ctatgtctac gacgactccc gctttaaaaa cgcgctgacc ggtgaactcg
    13141 atgaggtggg caatcgcatt gcgacctgga gctacgattc ccaagggcgc gcgacagggg
    13201 tcagccatcc cgattcgact cgtaatgcgc aattcagtta cggcgcgggg acaacctccg
    13261 tcaccgatag caaacgcacg acgacactga gtttttcttc agtcggcggc atggtgcggc
    13321 ccacctccag cagttctgtt gacggcaccg ccaatctggc ctgggatgcg ttgggcaatc
    13381 tgctgaaaga tagcggcgca ggtggcaata cagagtacgt gtacgacgag gtgggacggc
    13441 cggttcgcat aacaggcaat agcgcgtccg gcacctcgat caccacgatt cgctacgccg
    13501 acgcaacgag cctgcatccg tcgatcatcg cggcgcctgg cgttatccag tcagtcgttt
    13561 atgacgcacg gggtaatgcg accggtatga gcgaaacgcc gaccacggat agtacggggg
    13621 cgagtggatt cgacgcggtc aaatcggacg gcgtgacgcg tgcctacggc atggtctacg
    13681 acgcgtttaa ccggctcacg ttcgttcaga tgtatgaggg cagtgcactg gtcagtcagt
    13741 gggtggttac ccacgacgcc acgggcaatg tgttctcgat ctatcccaca gatgagtacg
    13801 tcgcgagcga aacaattgcg cgtgatgcca gtcatcgtcc tttgcgcggc tttaacccga
    13861 ccggcgattt taacttgcgc tatgaccggc gcgggcgcat cgaccttttc aagttcaacg
    13921 aatatgcgag cccggcaaac ggcggtgtgc gacgcgtgtt caaggtcaga ttcggctatt
    13981 caccagacgg ccaggttgtg tcgcgtaccg gcacggtcgc caagaacggc agtctcctcg
    14041 atctgaacga tggcacggat atcgcgattt ccagcggtga gatcgaccag tggatcgaca
    14101 actacaacct cggtgaatcc ccggtaggac cgccagccaa tctccagggc gcgaggcgtc
    14161 tactaggcga taacttcctg ggcacttcga ctgtgtgcag tggttgccat ttctcggctg
    14221 gcttgcccga cggcatactg cgcggcatcg tatttgtgtg gcggctggtg cagaatccag
    14281 cagtccggta cggcatcggg cagggtgtgc agaaggcgcg gcaaacgatc gagcgcatca
    14341 aggagcagtg taaaccggtc gttaaggaca cgtcggagat aaactgggga caaaatccta
    14401 atcagctgta tcacaccttt cggcacctcg aagagcaggg aattcctcag caacctgtca
    14461 tgaacgcgat ccgagaggat ctcgcggaga gaggttcgac aattcaattg ggggacaatg
    14521 cgactacgat gaccgtgacg gttgacggtc gagcaatcga ctatatcgca ttccgccagt
    14581 ctagcaacac gatacgagtg ggctcgattc gcccaaaatg aggaacgaac aaatgtcaag
    14641 accactctcg gcgaaggagc atgcgctcct caagtttctg atcgaggcaa atgagccgct
    14701 gtacggtgat cgaactaatc aatggaagac acaggttgaa acgtgtctgg tacgcgagat
    14761 cgattctcct tatttccttg cagtgattca cgaagaaagc atcgaaaaag caggatgcga
    14821 ctcaatcaca ctcggctacg aattggtggg cgtcgatcac ggtgtgccgg ttctaatcta
    14881 tgccgtggca atgaaaaccc catcagacta tttcatagat atcttcaatg tggaccgatt
    14941 agatggagag ccacttgtta actatcctga ggcgggcgac gggttaatga ttgtcgagcg
    15001 aaacaagagg gtcggtgggg cagacttgcg ccatctatat ggcggatcgc gttcgtaact
    15061 tgccctgtca tgcgacagtc agcgtaacac ctgactgtcg cagcacaatt cactttatgt
    15121 cgttggacag cgccccgtcc cactggactg acggcgcggg ttcctagctt ttcggcgtcg
    15181 cccgttttct ctctgcgaat ggatagtcca ctaacggtac gaaacggaga ttttctactt
    15241 gaccgtcgat ggcatacaga tcggagattc ggtttatcag caggacgtga gcgttgttca
    15301 cgtcggcttg atccgacctc cacggcaatt gcatcgcgga gaatatttat gcaacgaact
    15361 ttttttaaac ctcaagaacg ccggctactg gagtttcttc ttaaagtgaa cgagcctttt
    15421 tatggcgctt acgtttcccg gtggacggcc caattagaga catgcacggt tcgtgaagtc
    15481 aatgttccgt attgtctggc catcagtcac gatgaaacca gactttctgg aggaggcttc
    15541 actacgctcg cgcgtgagct ggtttgcctc gacgaagacg tgccagtgct gatttatgct
    15601 tgcgccgtgg agacgcagtc aggttatgtc ctcgactctt ttgacatcga tcgtctcgat
    15661 ggtgagcctc ttgcggtcta tcccgagcca ggcgatggcc tcatgatcat ggaagcggg
//
LOCUS       DATASH010000076         5011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4361752,
            whole genome shotgun sequence.
ACCESSION   DATASH010000076 DATASH010000000
VERSION     DATASH010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5011
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4361752"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VHV99_02235"
     CDS             complement(<1..452)
                     /locus_tag="VHV99_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HEX3634307.1"
                     /translation="MPNVPTHNWGLEQIVADLRASREELHRTRHPLGIRELPSREAVI
                     GIVAGLRAALFPTHYGAPDLTDETVDYYVGHTLESTLRLLAEQIRRALRFLPEHAETL
                     DVDLSARAFEVAREFGTQLPAIRALLVSDIQAAYTGDPAAQHITEILL"
     gene            complement(634..867)
                     /locus_tag="VHV99_02240"
     CDS             complement(634..867)
                     /locus_tag="VHV99_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634308.1"
                     /translation="MHFDAAFTHRGYLLNCAPARACDGTWQPYVVISRSSDGELVANR
                     FFPVDLRFPDEATAIAHARDWAVRWIDASSVTI"
     gene            1181..1633
                     /locus_tag="VHV99_02245"
     CDS             1181..1633
                     /locus_tag="VHV99_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3005 domain-containing protein"
                     /protein_id="HEX3634309.1"
                     /translation="MNSDINKPADASKQPGTTGPSTTELHNDRTHDSTVDTDGKNLEA
                     ARIAGHSPISPDEITTTNATLSNSVPEAHDGMAGFDSRIGGNHLLLALEPGYTVIDKG
                     MIEPQGVYSADHQFDDPRPVGSRSERGRIHYALNHLRPTRLIELHRVK"
     gene            1811..2446
                     /locus_tag="VHV99_02250"
     CDS             1811..2446
                     /locus_tag="VHV99_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="short chain dehydrogenase"
                     /protein_id="HEX3634310.1"
                     /translation="MHAQTLTEQHMKKIAVIGATGTIGHAVSAALKARHEVIEVGATR
                     GQYRVDSTDPASIERLFREIGKVDGVVTAMGKVHFGPLPEMSVEQFWVGLRDKLMGQI
                     NVVLTAQQYMNDGGSFTLTSGIIADEPIREGANATTVNLALEGFVRGAAIELPRGIRI
                     NLVSPTVLTEALDAYAPYFRGFEPVTAERAAQAYLRSVEGAQTGRVYRVGY"
     gene            complement(2584..3513)
                     /locus_tag="VHV99_02255"
     CDS             complement(2584..3513)
                     /locus_tag="VHV99_02255"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3634311.1"
                     /translation="MASDHRAARPSWSAPSPNENNDGAQHPSKRVKQRASVPGRIWHV
                     LVWGAMVFFLINVVLLIATVAVNSVATRWFGTLLPQGFTLHWYAQAWSDFQLASVLWV
                     TVEVVGAVVVLSVVLGVPAAYALARVQFRGKRTAMLVFLLPLMVPPVTYGIPMATAMY
                     KVGLAGTLSGVILANLVPALPFVILVMTPFIEQIDPNLESAARIFGANTFRYFRYVLL
                     PLLVPGMLAAGLLVLVRTIGMFELTFFTAGPATQTLVVALYYAVFSTGVRAPQSIDAM
                     AMIYMAITLIWVLIALQFVSPTQIVSRVKEQKR"
     gene            complement(3513..4400)
                     /locus_tag="VHV99_02260"
     CDS             complement(3513..4400)
                     /locus_tag="VHV99_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX3634312.1"
                     /translation="MNTPTLSPPGGPIAPRDAKAWLVAPALIFIVALFIYPFAYGLVL
                     SFRPMNGGGPWANYLTFFTDTSMWPTILVTLKLSVPATLINVGVSVPVAFALRRNSPY
                     QKFVTTLLVIPVTLGTVLIADGMLTYFGPNGWLPQALHGLHLYTDEVRLTHNFWGLLI
                     SLIVSGFPFAFLLTLSYVTGIDPTLASAAATLGASPWQQFRRIYLPLLVPGLTMAACL
                     SFVQAFSVFPSAVLLGAPAGPTRVMSIAAAEAAFESYDYSLASAIAMVMGFVQLLVVA
                     GMLGARRFFYSGPVSGGKG"
     gene            complement(4397..>5011)
                     /locus_tag="VHV99_02265"
     CDS             complement(4397..>5011)
                     /locus_tag="VHV99_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3634313.1"
                     /translation="AIVIEPPLILMDEPLSNLDTKLRIEMRAEIRRIHSQLERATLYV
                     THDQDEALSMADRIVVMREGVVQQIGTPKEVYTRPQNLHVARFMGYRNVAEFALEGTW
                     DEGVAVNANGVRLIGMPMAGFNSKRVSVALRPEDMERAAPGVENAFDALVTTVEYGGR
                     DSLLRVKTAFGDLWARIAGEFEEGERISLRVPPSRTLVYDGEAA"
BASE COUNT         1088 a         1591 c         1497 g          835 t
ORIGIN      
        1 caaagcagga tttcggtgat gtgctgcgcg gccgggtcgc ccgtgtacgc cgcctgaatg
       61 tcgctgacga gcaacgcgcg aatcgccggc agttgcgtgc cgaattcgcg cgcgacttcg
      121 aacgcacgcg cactgagatc gacgtccaac gtttcggcat gctcgggcag aaagcgcagt
      181 gcgcgccgga tctgttcggc tagcagccgc aacgtgcttt cgagcgtgtg cccgacgtag
      241 tagtcgaccg tttcgtcggt gagatcgggg gcgccgtagt gcgtgggaaa taacgccgcg
      301 cgcaaaccgg cgacaatgcc gatcaccgct tcgcgcgacg gcaattcgcg aatgccgagc
      361 ggatgccgtg tgcgatgcaa ttcttcacgc gaagcacgca agtccgcgac gatctgttcg
      421 agaccccagt tgtgggtagg cacgttaggc ataaaacacg gttcagggtg agggtgaggg
      481 tcccaagaga ttaccgcgtt ttgccgcggc tcgcgcagcg cgccacgatt gcccgcatgc
      541 acccctagcg cgcccgtgtg cttcagatgg tcatgctgct cgcgcagcgc cacacgatcg
      601 cccggcatgc aggcccgcat gcgggccctt gcttcagatg gtcacgctgc tcgcatcgat
      661 ccagcgcacc gcccagtcac gtgcatgcgc gatggcggtg gcttcgtccg gaaagcgcaa
      721 gtcaacgggg aaaaaacggt tggcgaccag ttctccgtcg ctcgagcggg aaatgactac
      781 ataaggttgc caagtaccat cgcacgcgcg cgccggcgcg caattcagca aatagccgcg
      841 atgtgtgaat gcagcatcga agtgcatgtg tcacccgccc tgacggcccc gtaaaatgtt
      901 ttgagtagcg cgtgcctgtg ggtgccggcc atgtaaaaac aaccgaacgc atgccaccaa
      961 cgcatgtctc caattgagcc gcgcttcgct cgtcggagta agatcatact ctgtagaaaa
     1021 tacgcgtcca ttaaaaaaaa cgaaaagaat tctgggcgca atcgcctgct gaaatgccgt
     1081 atgcgtaagc gcggcgcgac tcatgcgcac gcacgcatag tgcgaggcgg aacgggtttt
     1141 gctgcacttc aaccatgtcc tcttcaatgg agaatgcatg atgaacagcg atatcaacaa
     1201 gccagcagac gcgagcaaac agccgggcac caccggcccg agcacgacgg aactgcacaa
     1261 cgaccgcacg cacgacagca ccgtcgacac cgacggcaag aatctcgagg cggcccgcat
     1321 cgccggccac agcccgatct cccccgacga aatcacgacg accaacgcga cgctcagcaa
     1381 cagcgtgcct gaggcgcacg acggcatggc cggtttcgac agccggatcg gcggcaatca
     1441 tcttttgctg gcgctcgagc caggctatac ggtcatcgac aaaggcatga tcgagccaca
     1501 aggcgtctat tcagcagatc atcagttcga tgacccgcgc ccggtcggca gccgcagtga
     1561 gcgtggacgc attcactacg ccctgaatca tcttcggccc actcggctaa tcgaattaca
     1621 tcgggtgaaa taaggttttg cgcggcgcgt attcgtttga attgcgccgc ttgcgccgac
     1681 tattcagtca tttcgaaagc gcgcgccgtt tgaatgacgg tgcgcgtttc atcgtgggtt
     1741 agaaactcgg ggttgcagaa cctgcaagcc gaacaggctt tttccgtgcg ccgaacccag
     1801 acacgatcga ctgcacgccc aaaccttaac ggagcagcac atgaaaaaga tcgccgtgat
     1861 aggcgcaacc ggcaccatcg gccacgcagt cagcgcggca ttgaaagcgc gccatgaagt
     1921 catcgaagtg ggtgcgacgc gcggccagta tcgtgtcgac agcaccgacc cggccagcat
     1981 cgagcggctc tttcgcgaga tcggcaaggt ggacggcgtt gtcactgcaa tgggaaaggt
     2041 gcatttcggc ccactgccgg agatgagcgt cgagcagttc tgggtggggt tgcgcgacaa
     2101 gctgatggga cagatcaacg tggtgctgac ggcccagcaa tacatgaatg acggcggctc
     2161 gttcacgctg acgagcggca ttatcgccga cgaaccgatt cgcgaaggcg cgaacgcaac
     2221 caccgtcaat ctggccctgg aaggtttcgt gcgaggcgcc gcaatcgaat tgccgcgcgg
     2281 cattcgtatc aatctggtga gcccgacggt attgaccgaa gcgctcgacg cttatgcgcc
     2341 ctacttccgc ggcttcgagc ccgtgaccgc cgagcgcgca gcgcaagctt atctgcgcag
     2401 cgtcgaaggc gcgcaaacag gccgtgtgta tcgcgtcggg tattgaaccc ttagcaagca
     2461 agctcagttt cgtcgccccc gcatcctcca atccccaaga gggacttcct ccggggcgac
     2521 aaacttcttc ggagcgaggg acttcgttcg gggcctgctt ccgcgattcg gtgcctcgtt
     2581 gcttcagcgc ttctgctcct tcacgcgcga cacgatctgc gtcgggctga cgaattgcag
     2641 cgcgatcagc acccagatca gcgtgatcgc catgtagatc atcgccatcg cgtcgatcga
     2701 ttgcggtgcg cgcacgccgg ttgaaaatac agcgtagtac aacgcgacca cgagcgtttg
     2761 cgtcgcggga ccggctgtga agaacgtcag ttcgaacatg ccgatggtgc gcaccagcac
     2821 cagcagcccg gccgccaaca tgccgggcac cagcaagggc agcagcacgt aacggaaata
     2881 acggaatgtg ttcgcgccga aaatgcgcgc ggccgattcg agattgggat cgatctgctc
     2941 gatgaatggc gtcatcacga ggatcacgaa cggcagcgcc ggcacgagat tcgcaagaat
     3001 cacaccgctt agcgtacccg ccagaccgac cttgtacatc gccgtcgcca tcggaatgcc
     3061 atatgtcacg ggcggcacca tcaagggcaa caggaaaacc agcatcgccg tgcgcttgcc
     3121 acgaaactgc acgcgcgcga gcgcatacgc cgcgggcaca ccgagtacga ccgacaacac
     3181 caccaccgcg ccgaccactt cgacggtgac ccacaacacg ctcgccaact ggaagtcgct
     3241 ccacgcctgc gcataccaat gcagcgtgaa gccttgcggc aacaaggtgc cgaaccagcg
     3301 cgtcgccacc gaattcaccg cgacggtcgc aatcagcagc accacgttga tgagaaaaaa
     3361 caccatggcg ccccacacca gcacgtgcca gatgcggccc ggcacactgg cgcgctgctt
     3421 cacgcgcttg ctggggtgct gcgcgccgtc gttgttttca ttcggtgaag gcgcggacca
     3481 cgaaggacgc gcggcgcgat gatcgctagc catcagcctt tgcctcctga caccggaccg
     3541 ctgtagaaga agcggcgcgc accgagcatg ccggcaacca ccagcagttg cacgaagccc
     3601 attaccatcg cgatcgccga agcaagcgaa tagtcgtagc tttcgaacgc ggcttcggct
     3661 gcggcgatcg acatcacgcg cgtcgggccg gcgggcgccc ccagcagcac cgccgatgga
     3721 aacaccgaaa acgcctgcac gaacgataaa catgcggcca tcgtcaaacc gggcaccagc
     3781 agcggcagat agatgcggcg aaactgctgc cacgggctcg cgccgagcgt ggcggcggcg
     3841 ctcgcgagcg tcggatcgat gccggtcaca tacgacagcg tcagcagaaa cgcaaacgga
     3901 aaacccgaca cgatcagcga gatcagcagg ccccagaagt tatgcgtcag acgcacttca
     3961 tcggtgtaga ggtgcaatcc gtgcaacgct tgcggaagcc agccgttcgg tccgaagtac
     4021 gtgagcatgc cgtcggcgat cagcacggtg ccgagcgtga cgggaatcac cagcagcgtc
     4081 gtgacgaatt tctgataagg cgaattgcgg cgcagcgcga acgccacggg aaccgacacg
     4141 ccgacattga tgagcgttgc cggcacagag agcttgagcg tgacgagaat ggtcggccac
     4201 atcgacgtgt cggtgaagaa cgtcagatag ttggcccacg gtccgccgcc gttcatcggc
     4261 cggaacgaca gcaccaaacc atacgcgaac ggatagataa acaacgcgac gataaagatc
     4321 aaagccggcg cgaccagcca cgccttggcg tcgcgcggcg caattggtcc gcctggcggc
     4381 gacagcgtgg gcgtgttcat gcggcctctc cgtcatagac gagcgtgcgc gacggcggta
     4441 cgcgcaagct gatgcgctca ccctcttcga actctcccgc gatgcgcgcc cacaagtcgc
     4501 cgaacgcggt cttcacccgc agcagtgaat cgcggccgcc gtactccacc gttgtcacca
     4561 gcgcgtcgaa ggcgttttcc accccaggtg ccgcacgctc catatcctcg gggcgcaacg
     4621 cgacgctcac gcgcttgctg ttgaaaccgg ccatcggcat gccgatcaga cgcacgccgt
     4681 tggcgttgac cgcaacgccc tcgtcccacg tgccttcgag cgcgaattcg gccacgttgc
     4741 gatagcccat gaaccgagcc acatgcaaat tctgcggccg cgtatagacc tctttcggcg
     4801 tgccgatctg ctgcaccacc ccctctctca tcacgacgat gcggtcagcc atggaaagcg
     4861 cttcgtcctg atcgtgcgtg acgtagagcg tcgcgcgttc gagctggcta tggatgcggc
     4921 gaatctccgc gcgcatttca atccgcagtt tcgtatcgag attcgacagc ggttcgtcca
     4981 tcaggatcaa cggcggctcg atcacgatcg c
//
LOCUS       DATASH010000077         2715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1789693,
            whole genome shotgun sequence.
ACCESSION   DATASH010000077 DATASH010000000
VERSION     DATASH010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1789693"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VHV99_02270"
     CDS             <1..184
                     /locus_tag="VHV99_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3634314.1"
                     /translation="LESRARQLQRSLLTLDTRTGDKAEPLYAALGYQTVGVIPQFCRD
                     TQEERLDSTTIMYKAL"
     gene            complement(430..825)
                     /locus_tag="VHV99_02275"
     CDS             complement(430..825)
                     /locus_tag="VHV99_02275"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX3634315.1"
                     /translation="MSDFPIDNPFLESLGVRLKQWRDGYAELYMPIDASKLNRQGVLQ
                     GGAIATLLDAAAGYAGVYAEAGQPVRHAFTLSLTTNYLDKGLGESVTAKGFLERQGRS
                     IFFSRSEAWVDDKLLIASAQGTFKYSRTR"
     gene            1223..2452
                     /locus_tag="VHV99_02280"
     CDS             1223..2452
                     /locus_tag="VHV99_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3634316.1"
                     /translation="MSPATPLDGAPHWQRNLYVCLFGSFTTIVAMTLLLPFLPLYVQQ
                     LGVHSAAAAVQWSGIAFGATFLAAALVSPLWGRMADRYGRKLILIRASLGMAVVMSLL
                     GLAQNVWQLVGLRFLAGLVGGYASGATVMIATQTPRGRTGWALGTLASGVMAGNLVGP
                     LIGGALPPLIGIRNTFFIAGAMIFCAFIATTLLVKEQPLARSRSTSHEPDAATGWASV
                     PDLRPVLAMLFTAGLLMFANMSIEPIITVYVGQLVHDARHVTLVSGFVMSASALGSVL
                     AASRVGRLADRIGAPKVIVACLAVCAALLVPQAFVTSGVQLVALRFLMGLALAGLLPS
                     ITSVIRHNVPDRSAGYILGYATSANYAGQVAGPLAGGFVGAHFGMQSVFLITSGLMLC
                     GALLNGWVFGGGAPHGE"
BASE COUNT          429 a          830 c          863 g          593 t
ORIGIN      
        1 actggaaagc cgtgcgcgcc agttgcagcg cagcctgctg acgctcgata cgcgcaccgg
       61 cgacaaggcc gagccgctct atgccgcgct gggttatcag acggtcggcg tgattccgca
      121 gttttgccgc gacactcaag aggagcggct tgactcgact actatcatgt ataaggcgct
      181 ttgagagaga tgtcgcttgg gggggcgctt gcgcagccag gatgcgcgtg gtgcgagcgg
      241 tgacggttcg gtagagcgaa gcagcccagc caacacgcgc gcgaggaccg ttcagtaaag
      301 cgaggcgcag cgaggaaccg tgatgcatga ccgggatgcg cgtgatcagg ttgggtgacc
      361 gactcacgcg acgaaaggcc ggagcttctg gccgatcgcc tttcaaactc ttcaaactct
      421 tcaaactctt caacgcgtcc tggagtattt gaacgtgcct tgcgcgctgg cgatcagcag
      481 cttgtcgtcc acccacgctt cgctacgcga gaagaaaatc gagcgtccct gacgctccag
      541 gaagcccttc gcggtcaccg attcgccgag tcccttgtcg agatagttgg tggtcagcga
      601 gagcgtgaag gcgtgccgta ccggctgccc tgcttctgca taaacccctg catatcccgc
      661 cgccgcatcg agcaaggtcg cgatcgcgcc gccttgcagc acgccctggc gattcagttt
      721 gctggcatcg atcggcatat acagttcggc ataaccgtca cgccattgct tcaaccgcac
      781 gccgagcgat tcgagaaacg ggttgtcgat gggaaagtcg gacatgtgct ggcctttgtg
      841 tcatcagtga agaatgcgga gcgcgcacgg cggcgcttca cgagctccca gcataccagg
      901 gctggccgcc ggtcgttggt tgtgcgggcg caggctttat cggccagggc tactgccata
      961 gtgagccgat ctggattgcc ggcacgcttc atcaacctcg tttgctggga ttgacgaatg
     1021 gacatcgctc attgggccat gcgattggcc cacttcatca atccacttca tcaatccact
     1081 tcatcaatcc gctccatcag ccgacttcat caatccgctc catccgctga cttcgccgac
     1141 tcgattccgc gcgctcaggc acaatcgtcc tttcgccgct tccggcccaa tgtgtctaca
     1201 tcccgtttca catcttgcct cgatgagtcc agccacaccc cttgacggcg cgccgcactg
     1261 gcagcgcaat ctctatgtct gcctgttcgg ctccttcacg acgatcgtcg cgatgaccct
     1321 gctgctgccc ttcctgccgc tctatgtgca gcagctcggc gtgcattcgg cggcggccgc
     1381 cgtgcaatgg tcgggcatcg cgttcggtgc gacgttcctt gccgccgccc tcgtgtcgcc
     1441 gctgtggggg cgcatggccg accgctatgg acgcaagctg atcctgattc gagcgagcct
     1501 cggcatggcc gtcgtgatgt ctttgctcgg gctcgcgcag aatgtctggc aacttgtcgg
     1561 cctgcgcttt ctggcgggcc tggtcggcgg ttatgcgtcg ggcgccaccg tgatgatcgc
     1621 cacgcaaacg ccacgcggac gcaccggctg ggcgctcggc acgctggcct cgggcgtgat
     1681 ggcgggcaat ctcgtcggcc cgttgatcgg cggcgcacta ccgccgctga tcggcattcg
     1741 caatacgttt tttatcgccg gtgcgatgat tttctgcgct ttcattgcga cgacgctgct
     1801 cgtcaaggag caaccgctcg cgcgctcgcg ctcgacatcg cacgaacccg atgcggcgac
     1861 tggctgggcc agcgtgccgg atttgcggcc cgtgctcgcg atgctgttca ctgccgggct
     1921 gctgatgttc gccaacatgt cgatcgagcc gatcatcacc gtctatgtcg gccagctcgt
     1981 gcacgacgcc aggcacgtga cgctggtgtc gggcttcgtg atgtcggcgt cggcattggg
     2041 cagcgtgctg gcggcctcgc gtgtcggccg cctcgccgac cggatcgggg cgccgaaggt
     2101 gatcgtcgcg tgcctcgcgg tgtgtgcggc cttgctggtg ccgcaggcgt tcgttaccag
     2161 cggcgtgcaa ctcgtggcgt tacgtttttt gatggggctc gcactggctg gcttgttgcc
     2221 gtcgattacc agcgtgatcc ggcataacgt gccggaccgt tccgccggct atatcctcgg
     2281 ttatgcgacc tccgcgaatt acgccggaca ggtggccggg ccgctcgcgg gcggctttgt
     2341 cggcgcgcac ttcggtatgc agtcggtgtt tctgatcacc agtgggctga tgctgtgtgg
     2401 cgcgttgctg aacggatggg tgttcggagg gggggcgccg cacggcgaat agctgggggc
     2461 gggcgcgcct ggggcgttgg tcttttcttg agctggggtt ttggcctttc cttgaattgt
     2521 tagtggtcta ttagcgttgc ccctgtgcgg ggcggcacat acttttcttt gcctgccgcg
     2581 taactattca gcgcttgtaa actgctacca ggctgtgcat agtgagggat atgacgaaga
     2641 tgcccgacat caaggatctg acaccggagc agaaggacgc actgatcgtt gatctggtga
     2701 ggcgtctgaa cgagc
//
LOCUS       DATASH010000078         9517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2976306,
            whole genome shotgun sequence.
ACCESSION   DATASH010000078 DATASH010000000
VERSION     DATASH010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 9517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9517
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2976306"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..124
                     /locus_tag="VHV99_02285"
     CDS             <1..124
                     /locus_tag="VHV99_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013690721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VapC toxin family PIN domain ribonuclease"
                     /protein_id="HEX3634317.1"
                     /translation="SEPAVDKLIAATALACDLVVVTRNVADFAAPGIRVVNPFD"
     gene            223..663
                     /locus_tag="VHV99_02290"
     CDS             223..663
                     /locus_tag="VHV99_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634318.1"
                     /translation="MPTRSHFYCKAALLALAVASLAGCASTPAEPVPFKEVPQARIVQ
                     PGYTQPATGLVAVDVRRERSRDVIVRFRDALVYIDGEQVTDLMNGEHVVFYLSPGVHR
                     IGVSTQFDPVVEVHFTVTADTRYTNRALVTFGDDHRIALRRVAQ"
     gene            880..1857
                     /gene="corA"
                     /locus_tag="VHV99_02295"
     CDS             880..1857
                     /gene="corA"
                     /locus_tag="VHV99_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HEX3634319.1"
                     /translation="MLINCAAYQDGRKLADIDIDSISDYVARPECFVWVALKDPAPEE
                     LAVMKHEFGLHELAIEDAQYGHQRPKIEEYGESLFAVMHTVETDEEGELLIGEVDVFV
                     GHNYVLSVRRGTRTGFQNVRARCEREPQLLKEGSAFVLYALADDIVDRYFPIIEAMNT
                     EIEALEDRIFDRNSSSASRAIIEDLYSLKRRLVILQHHIAPLQEAIGKLTGGRIPSIC
                     EGMQAYFRDVYDHLERIVRTIDGRREMIVTAVQVNLGMISLAESEVTKRLGSFAALFA
                     VPTMIAGIYGMNFQSIPELHYKYGYPICLAVMLTVDLILYWRFRKAGWL"
     gene            2203..3165
                     /locus_tag="VHV99_02300"
     CDS             2203..3165
                     /locus_tag="VHV99_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX3634320.1"
                     /translation="MSHRLRRRMLAAAVLVTATAALPFSSAYAQTASAHKPKVALVMK
                     SLANEFFLTMENGAKDYQKHNPSQFDLITNGIKDETDTANQIRIVEQMIVSKVDAIVL
                     APADSKALVPVVKKAVDAGIIVVNIDNRLDSDVLKSKDLNVPFVGPDNRKGAQKVGDY
                     LAKKLKSGDEVGIIEGVSTTTNAQQRTAGFKDAMQAVGAKVVSVQSGDWEIDKGNAVA
                     SAMLNEYPNLKALLAGNDNMAIGAVSAVRAAGKQGKVLVVGYDNINAIRPMLKDGRVL
                     ATADQYAAKQAVFGIDTALKAISEHKKQSQLSGVVETPCDLVTR"
     gene            3517..3720
                     /locus_tag="VHV99_02305"
                     /pseudo
     CDS             3517..3720
                     /locus_tag="VHV99_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002473.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            4005..5045
                     /locus_tag="VHV99_02310"
     CDS             4005..5045
                     /locus_tag="VHV99_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX3634321.1"
                     /translation="MATIKDVAAVAGVSFTTVSHVVNNSRPVSADVRAKVEHAIRQLH
                     YVPSAVARSLKARATATIGLVLPNSTNPYFAEMARGIEDGCSRNGYCLFFCNSDDDPV
                     KQRNYLRVLQEKRIDGLIVASAGDDAVLAQTLADAHEPLVVLDRNIEGLNADLVQIDH
                     EKGGYLATRHLLELGHVRIGCITGPVSTAVSAMRVHGFIRAMTERGIEIPANAIVESD
                     FSGTGGHRAAGQLFDTLKPSAIFAGNDMMGIGALRAAAERNISVPRDCSIIGFDDIEL
                     GRFTYPSLSTVGQSVRALGDMAAQTLIERIAGSSSGSPRAPGRRRVVSPRLIVRESTA
                     TWAGVTRRDLAA"
     gene            5492..7309
                     /locus_tag="VHV99_02315"
     CDS             5492..7309
                     /locus_tag="VHV99_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018435600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX3634322.1"
                     /translation="MLNKGITIKARIGLTMAFLAALLVAIGVFGLFGMSRSNAAYEDT
                     FTNAMPGAVDIGNAELFAARERLVLDRAAFMAGTPEVAPTIERARSMRVTSETWWKKY
                     MDLPRGAEENRLAQDVVAKRGVLYQQLDAFAATIMANDPSNLVEGAKRVQAAYNELSI
                     SDEALRQFQFTSAKEDFDAAQSSAEVFRMVSAGALLVGVLAAVLSYLTLSRAIGRPLE
                     EALGHFAAIAAGDLHRPVVVTSRDEMGQLLDGIAKMQRSLTETVRTVRGGSESIATAT
                     RQIAAGNSDLSSRTEEQASALQETASSMEQLTGTVKQNADNARQASSLAANASEIANR
                     GSAVVGQVVGQVVGTMGDINQSSAKIADIISIIEGIAFQTNILALNAAVEAARAGEEG
                     RGFAVVAGEVRSLAQRSSAAAKEIKELIDTSVERVQSGSALVDEAGRTMTEIIGAVQR
                     VTDIMGEIAAASEEQSSGIDQVARAVTQMDEVTQQNAALVEEAAAAASSLEDQAGKLR
                     AAVAVFQLDESGYKVPVSAAPKRAAAPGRPTAARKVSPSALQSASQAVSQAASPPRAQ
                     APAAARAPAAHPAPAAAAPARTPAKAVASAGNDQDWETF"
     gene            7887..8648
                     /locus_tag="VHV99_02320"
     CDS             7887..8648
                     /locus_tag="VHV99_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HEX3634323.1"
                     /translation="MTPYDLANRLVEARRQHRTIDAPPPDSLPPDAATAYAIQQAVIA
                     GLGDSTGGWKIGARAPGGAAAGAPIPASLVVPSPARVAHSGFFRVWVELEIAFRFGEA
                     IEPRGRAYARDEVLSKVGVVLPTIEIVDSRFAEWPNIAPLAQLADAQNNGALVTGHPV
                     AYVSLARSFDFVSPELELSVDGTSLVPEVTGNQAGDPRELLVWFVNHCAAMGITIEPE
                     WTITTGSYVGAHNVNKPGIVRGHIDGLGEVEIELT"
     gene            complement(8759..9034)
                     /locus_tag="VHV99_02325"
     CDS             complement(8759..9034)
                     /locus_tag="VHV99_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634324.1"
                     /translation="MRRIVKRFVNAPAILFRAIRVERKGSPRFAPHAWRLAINRVGSM
                     VSRSYRAHKLDHGSAMGRCIGVYGKTPHADAVNVKRPPAGSRLFDAS"
BASE COUNT         1835 a         2897 c         3060 g         1725 t
ORIGIN      
        1 atcggagccg gccgtcgaca aactgatcgc tgccacggcg ctggcatgcg acctcgtggt
       61 cgtgacgcgc aatgtcgcag attttgcagc gcccggcatt cgtgtcgtca acccgttcga
      121 ctgaccgtcc gtccccagta aaaaggcaag agcgccattg gcattgccct gggcgcctct
      181 aacacagaag caacaccaca accaacaaca gggagacccc acatgcccac gcgctcgcac
      241 ttctactgca aagccgcgct gcttgcgctt gccgtcgcgt cgctcgccgg ttgcgcgtcg
      301 acccctgccg agcccgtgcc gttcaaggaa gtgccgcaag cgcgcatcgt ccagcccggt
      361 tatacgcagc ccgcaaccgg actggtcgcc gtggacgtgc ggcgcgaacg ttcccgcgac
      421 gtgatcgtcc ggttccgtga cgcactggtg tatatcgacg gcgagcaggt caccgatctg
      481 atgaacggcg aacacgtagt cttctatctg agtccgggcg tgcatcgcat cggtgtctcc
      541 acgcaattcg atccggtcgt ggaggtgcac ttcacggtca cggccgacac gcgttatacg
      601 aaccgtgcgt tggtcacctt cggcgacgat catcgcatcg cgttgcgcag ggttgcacaa
      661 tagggcaggg caggacccgg tcaggccgaa gtgcgtcgac ctgtgccgaa ccgcgccccg
      721 ttgtcaccgc aggtaagctt taacaattgg atacatgaca ttcatacata gcccttaaca
      781 tgacggccgt cagggtgttc actcatgcag aagatccgcc gccgggaatg cgcacagtgc
      841 gacgccagcc ggtctgttcc gttttcaagg taatccaaca tgctgatcaa ttgcgccgcc
      901 tatcaggacg gccgaaaact ggctgacatc gatatcgata gcatcagcga ctacgtggcg
      961 cggcccgagt gcttcgtctg ggtcgcgctg aaagacccgg cgccggagga attggccgtg
     1021 atgaagcacg agttcggcct gcacgaactc gcaatcgagg acgcgcaata cggccatcag
     1081 cgtccgaaaa tcgaggagta cggcgaatcc ctcttcgccg tgatgcacac ggtcgaaacg
     1141 gacgaagagg gcgaactgct gatcggcgag gtcgacgtgt tcgtggggca caactatgtg
     1201 ctgtcggtac gccgcggcac gcgcaccggg ttccagaacg tgcgtgcccg ttgcgagcgc
     1261 gagccgcaat tgctcaagga aggttcggcg ttcgtgctgt acgcgctggc ggatgacatc
     1321 gtcgatcgtt attttccgat catcgaggcg atgaacactg agatcgaagc gctcgaggac
     1381 cgcatcttcg accgcaacag ttcgtcggca tcgcgcgcga tcattgagga tctgtactcg
     1441 ttgaaacgtc gcctcgtgat cctgcagcac cacattgcgc cgctgcagga agcgatcggc
     1501 aaactgacgg gcggccgcat tccgagcatc tgtgaaggca tgcaagccta tttccgcgac
     1561 gtctacgacc acctcgaacg gattgtcaga accatcgacg gccgccgcga aatgatcgtc
     1621 accgcggtgc aggtcaatct ggggatgatc tcgcttgccg aaagcgaggt gaccaaacgg
     1681 ctcggctcgt tcgccgcctt gttcgcggtg ccgaccatga tcgccggcat ctacgggatg
     1741 aacttccaga gcattcccga gctgcattac aagtatggtt atccgatctg tcttgccgtg
     1801 atgctgaccg tggatctgat tttgtactgg cgctttcgca aggctggctg gctttgacag
     1861 acgttggcca gcgccccggc gttgctgctg tttgcaccgt gcgcaacaga agtccagcat
     1921 aggcgcccgc tggccgctga actggcagcc ggcggcgaat ctcccccgcg cggaccgggt
     1981 gcggcaccct tgccgacacg cagcctgctg gtgtgcgtat ccgccgcgtc gggtagaacc
     2041 catcgaacgc gatgcatcga cgcactttcc gcgcaccgat gcatcgcaac acctcaaatc
     2101 gttgacactc tgaactcggc acgctgagaa tagccgtcgc aaaccgtttg cgcatcacta
     2161 aaaatacggc tcgagcgcga gccggataaa cctggagatt ccatgagcca tcgccttcgc
     2221 cgccgcatgc tggcggccgc agttctcgtc accgccactg ccgctttgcc gttttcctcc
     2281 gcttatgcgc aaaccgcgtc cgcccataag ccgaaggtcg cgctggtgat gaagtcgctc
     2341 gcgaacgagt tcttcctgac catggaaaac ggtgccaagg attaccagaa gcacaacccc
     2401 tcccagttcg acctgatcac caacggcatc aaggacgaga ccgataccgc gaaccagatc
     2461 cgcatcgtcg aacagatgat cgtctcgaag gtcgatgcga tcgtgctggc cccggccgac
     2521 tcgaaggcgc tggtaccggt tgtcaagaag gccgtcgatg cggggatcat cgtcgtgaac
     2581 atcgacaacc ggctcgactc ggacgtgctc aagtccaaag acctgaacgt gccgttcgtc
     2641 ggcccggata atcgcaaggg cgcgcagaag gtcggcgact acctggccaa aaagctgaag
     2701 tcgggcgatg aagtcggcat tatcgaaggc gtgtcgacca ccaccaacgc gcaacaacgc
     2761 actgcgggct tcaaggacgc catgcaggcc gtcggcgcca aggtcgtctc ggtgcaatcc
     2821 ggcgactggg aaatcgacaa gggcaatgcg gtggcgtcgg ccatgctcaa cgagtatccg
     2881 aacctgaagg cgctgctggc cggcaacgac aacatggcga tcggcgcggt ctcggccgtg
     2941 cgcgcggcgg gcaagcaggg caaggtgctg gtggtcggct acgacaacat caacgcgatc
     3001 aggccgatgc tcaaggacgg ccgcgtgctc gccaccgccg atcagtacgc cgccaagcag
     3061 gccgtgttcg gcatcgacac cgcgctgaag gcgatcagtg agcacaagaa gcagtcgcag
     3121 ttgtccggcg tggtggaaac gccgtgcgat ctggtgacca ggtaagcgtc cggcagcaag
     3181 taccgattac gcactgtaag aagcatcgca acacgcccgg gcacgcggat tcgagttcgc
     3241 gccggtgcag ttgaatagag ggtgaaaaac acggggattg cgttaattca ataaacgctc
     3301 aagaaacctc gcgcgccgcc gttaattcca gaggtaagcg tcatgacggc ggctgcgccg
     3361 gcgggagagt gcgtagtgga aagcgcccgc gcattccgcg cattgggccg gtatggcacg
     3421 tggctgtcgc tggttgctgg ttgcggcttg cgccggctac cgcagccgcg aaacgcatgc
     3481 tgcatgcccc gacgccaatg aagcaaggag tctgtcatga ccgatcaatc ggtgcgagga
     3541 ggatcgagtg gatcgagtgg gaagcccgac gcggtgaatg tggccagcgt aacgccgccc
     3601 gccaacctgt ccgcgtcgcc ggcaagcggc aagccggccg gcacgcggct cggcttttcg
     3661 aactccctgg gccttctccg tgcgttgctg gcgatgatcg cattcgattt gctgtcgaat
     3721 ccgatcgtgc ccggcatctc gccggcgttc ctgatcgcgg cggcggtgaa cggtgctcgc
     3781 ggccggcctt tgcgcagatc tgcgcgcagg agccggccag gcggataatg accggcgcgg
     3841 tgatcgtggt ggcggtggtg ctggatacct atcgcagccg tcgtaagcgc gcgtgatggc
     3901 gagcgcggtt gtcattcatg ggtttggtct gtcttcgatg aagatgtcgg tgggtcgcca
     3961 gatggcgtgg ctggcgggaa caacaggcag gagagcaaca ggttatggcg acgatcaagg
     4021 atgtagcggc cgtggcgggc gtgtcgttca cgacggtatc gcacgtggtg aacaactcac
     4081 ggccggtatc ggccgacgtg cgcgcaaagg tcgaacacgc gattcgtcag cttcactatg
     4141 ttccgtcggc ggtggcccgc tcgctcaagg cgcgggcgac cgcgacgatc ggactggtgt
     4201 tgccgaacag cacgaacccg tatttcgcgg aaatggcgcg cggtatcgag gatggttgct
     4261 cgcgcaacgg ctactgcctg tttttctgca attccgacga cgatccggtc aagcagcgta
     4321 actatttgcg cgtgctgcag gaaaaacgta tcgacgggct gatcgtcgcc tccgcaggcg
     4381 atgacgcagt gctggcgcag actcttgcgg atgcgcacga gccgctggtc gtcctggacc
     4441 gcaatattga aggcttgaac gccgacctgg tgcagatcga ccatgagaag ggcggttatc
     4501 tggcgacgcg tcatctgctc gaactggggc acgtgcggat cggctgcatc accgggccgg
     4561 tgagcacggc ggtcagcgcc atgcgggtgc acggctttat ccgcgcgatg accgagcgcg
     4621 gcatcgagat tccagccaac gcgatcgtcg aaagtgattt ttcggggacg ggcggccatc
     4681 gcgcggccgg gcagttgttc gacacactga agccgtcggc gatttttgcc ggcaacgaca
     4741 tgatgggcat cggcgcgctg cgtgccgcgg ccgagcgcaa tatcagcgtg ccgcgcgatt
     4801 gctcgatcat cggtttcgac gatatcgaac tgggtcgctt cacctatccg tcgctctcga
     4861 cggtgggtca gtcggtccgc gcgctcgggg atatggccgc gcagacgttg atcgaacgta
     4921 tcgccgggtc ttcgtcgggc agcccgcgcg cgcccggacg gcggcgtgtc gtgtcaccgc
     4981 gcctgatcgt gcgcgaatcc accgcgacgt gggccggcgt caccaggcgc gatctggccg
     5041 cctgatcgag caccgggtta ctggcgcgag gcctcaaagg cttcgcgtcc ggtctttcgc
     5101 atagtccggc cgcggctctc agcttgagcg tgcgccagaa ttgggcgcaa gagcttgcgc
     5161 ccgtcgtcaa acgagtcatt gccttcgtgc aactcagccg cttcgcccgg cccctcctgc
     5221 gtccggtcag ccgcccgagc cgcctgacat caatttcctg aatgtccggg ttccgcgcgg
     5281 cctttcccgg gtgctgcctg tcagtattct ggaattcttt ccggttgctt tcagtgcctt
     5341 tcttcacacc ataaagattc ttcaagtatg cggatgaaat gccgtaaacg cggctattag
     5401 agcgcggtat taaccagcgc catccacgca gacgccactt gctagccgcg ctgcgcacgc
     5461 agcctcttat ttgccaagca caggaacaag catgttgaac aaaggcatca cgatcaaggc
     5521 gcggattggc ctcacgatgg cttttctcgc cgcgctgctg gtcgccattg gcgttttcgg
     5581 tttgttcggc atgagccgct cgaatgccgc gtacgaggac actttcacta atgcgatgcc
     5641 aggcgcggtc gatatcggca acgccgaact ctttgccgcg cgcgaacggc tggtgctcga
     5701 ccgggcggcg ttcatggccg gcacaccgga ggtagcgccg acgatcgagc gcgcgcgttc
     5761 gatgcgcgtc acgtccgaga cgtggtggaa aaagtatatg gacctgccgc gcggcgccga
     5821 ggaaaaccgt ctcgcccagg acgtggtggc caaacgcggc gtgctgtatc agcaattgga
     5881 cgcgtttgcc gcgaccatca tggcaaacga cccgagcaac ctcgtcgagg gcgccaagcg
     5941 cgtgcaggcg gcgtacaacg aactctccat ctccgatgaa gcgctgcgcc agttccagtt
     6001 cacctcggcc aaggaagatt tcgacgccgc gcaaagcagc gctgaagtgt tccgcatggt
     6061 gagcgccggt gccctgctgg tcggcgtgct cgcagcggtg ctgtcctacc tgacgcttag
     6121 ccgcgcgatt ggacgtccgc tcgaagaagc cctcggtcat tttgccgcga tcgccgcagg
     6181 cgatctgcat cgcccggtgg tggtcacctc gcgcgacgaa atggggcagt tgctcgacgg
     6241 tatcgccaaa atgcagcgca gtctcaccga aacggtgcgc acggtgcgcg gcggcagtga
     6301 atcgatcgcg acagccacgc gccagatcgc ggcgggcaat agcgacctgt cctcgcgcac
     6361 ggaagagcag gcttcggcct tgcaggaaac cgcgtcgagc atggaacagc tgaccggcac
     6421 ggtcaagcag aacgccgaca acgcccgcca ggcgagttcg ttggccgcca atgcgtccga
     6481 gattgccaac cggggtagcg cggtggtcgg ccaggtggtc ggccaggtgg tcggcacgat
     6541 gggcgacatc aatcagagct cggcgaagat cgccgacatc atttcgatca tcgaagggat
     6601 tgcgttccag accaacatcc tggccctgaa tgcggccgtg gaggcggcgc gagccggcga
     6661 agaagggcgc ggtttcgcgg tggtggcagg cgaagtgcgc agcctcgcac agcggtcgtc
     6721 ggcggcggcc aaggaaatca aggagttgat cgacacctcg gtggagcgcg tgcagtccgg
     6781 ttcggcgctg gtcgatgaag ccggccgcac catgaccgag atcatcggcg cggtgcagcg
     6841 cgtgactgac atcatgggtg agatcgcggc ggcctcggaa gagcaaagca gcggcatcga
     6901 ccaggtggcg cgcgcggtca cgcagatgga cgaggtgacg caacagaatg ccgcgctggt
     6961 cgaagaagcg gccgccgcag cctcgtcgct cgaagatcag gccggcaagc tgcgggccgc
     7021 ggtcgcggtg ttccagctcg acgaaagcgg ctacaaggtg ccggtgagcg cggcgccgaa
     7081 gcgcgcggcc gcgccgggcc gtccgacggc ggcacgtaaa gtgtcgccat ccgctttgca
     7141 gagtgcttcg caagccgtgt cgcaagctgc ttcaccaccg cgtgcgcagg cccccgcggc
     7201 agccagggca ccggccgcac acccagcgcc ggctgccgct gcgccagcga ggacgcctgc
     7261 caaggcggtg gcgagcgccg gcaacgatca ggattgggag actttctgat cggcagtcta
     7321 tgggaagcgc ggcacggcgg aacgcggcaa tgcgtgcggg cgtgcgggcg tgctgcgtca
     7381 gccgagcgtt ctgaacgcgc gaaagtgcca agtctgcccg cgcttgtcat gcactgaggt
     7441 gctgcgtctc ccgcgcttct aacgccggac gtgctgcgtc tgcccgcgct tcgaacgcgc
     7501 ggacgcatgc catccttgcc acgccttgag cacgacaccg aatccctgca gcggtcatgc
     7561 acccgccacg ccgctgcaac attcgttccc gatgaagcat tggccgtaat cacactggtg
     7621 cggatcgccc cgcatgacga caaagaccgc gattgcgcat gcaaacgcgg tcttttttca
     7681 tggtctgcgg actaagtaac gttcacaagc acagtccacg ccctggcggg cgtcgtgatc
     7741 cggtacattg acgggcgcga ctacgacttg catcggccac tcgccgcttc aggcaatcaa
     7801 cgcgcggaac gtgagcccgc catcttcaac cgctgcaact cagtgcggca acctgccgct
     7861 caacgcgcca gccataaagg accgacatga cgccatacga cctcgcgaac cgcctcgtcg
     7921 aagctcgccg gcagcatcgc acgatcgatg cgcccccacc cgacagcctg ccgcccgacg
     7981 cggcaaccgc atatgcgatc cagcaggcgg tcatcgcggg ccttggcgac tcgacgggtg
     8041 ggtggaagat cggcgccagg gcgccgggcg gggctgcggc tggcgcgccg attccggctt
     8101 cgctggtggt gccgtcgccc gcgcgggtag cccattcggg cttcttccgg gtatgggtcg
     8161 agctggagat cgcctttcgt ttcggcgagg cgatcgagcc gcgcggccgg gcctatgcgc
     8221 gcgacgaggt gctctccaag gtgggcgtcg tgctgccgac catcgagatc gtcgatagcc
     8281 gttttgccga atggcccaat attgcaccgc tcgcgcaact ggctgatgcg cagaacaatg
     8341 gcgcattggt caccgggcat ccggttgcct atgtgagcct cgcacgcagc ttcgatttcg
     8401 tatcgcctga actggagctg agtgtcgacg gcacctcgct cgtgccggaa gtcaccggta
     8461 atcaggccgg cgatccgcgc gaactgctgg tgtggttcgt caaccattgc gcggccatgg
     8521 gcattaccat cgagccggaa tggaccatta ccaccggttc atatgtcggt gcgcacaatg
     8581 tgaacaagcc gggcatcgtg cgcggccaca tcgacggtct gggggaagtg gagattgaac
     8641 tgacctgagc gtgtaacgct gtacttggtg acggcgtgct tgtaatgaag tgttttgttg
     8701 cggttcttgt aactggcgcc ttgtaatggg tccgtgtaac cgtccttgta accgtccatt
     8761 acgaagcatc aaacaaacgg ctgcccgcgg gcggccgttt tacattcacg gcatccgcat
     8821 ggggcgtttt gccgtataca cctatgcagc gtcccattgc gctgccgtga tccaatttat
     8881 gagcgcgata gctgcggcta accatgctgc cgacgcggtt aatggcaagc cgccaggcgt
     8941 gaggagcaaa gcgcggcgat cccttgcgtt cgacgcgaat ggcgcggaac aaaatcgccg
     9001 gtgcgttgac gaaccgcttg acgatgcgcc gcataacaga gccttagggt gagctgggac
     9061 gaacgaacat gtaacacagc gtttgctctt gtagaccaga tggcgatgcc gcgcgttgat
     9121 gaaaaaagta cccgggcacg gaaaacgcat aacgtccgcg ttcaatgcga caaagtgtgc
     9181 gcatgcagac aggctgaccg gtatcgcagt gagatcgttc gatgaacgcg gcgattcgag
     9241 taacggagca tggcgagttg cgcggggctg cacaccaggc agacggtgcc gccgaattgc
     9301 gacgacaatg acggcaatca cggcagtgga gaggccgcaa cgcaggccgg caatcgctgc
     9361 acattcgctg tgatgcgagc gcaagattgt aggtgagcga atctgtgttt ccaagtgagg
     9421 aatgttggga ggcgggtacg gcgcgatttt cgcaacgtag ttcgtgaaaa aaaatttaaa
     9481 agggtttagg atgaattcga gcgatgtcgt ttccgaa
//
LOCUS       DATASH010000079         2318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1196605,
            whole genome shotgun sequence.
ACCESSION   DATASH010000079 DATASH010000000
VERSION     DATASH010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1196605"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1092)
                     /locus_tag="VHV99_02330"
     CDS             complement(<1..1092)
                     /locus_tag="VHV99_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008645473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX3634325.1"
                     /translation="MPRKTPIERYRNIGISAHIDAGKTTTTERILFYTGVTHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWKGMAGNYPEHRINIIDTTGHVDFTIEVERS
                     MRVLDGACMVYDSVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGADFFRVQRQISE
                     RLKGTAVPVQIPVGAEDHFQGVVDLVKMKAIYWDDASQGIKFEYRDIPAELAATAKEW
                     HDKMVEAAAEANETLLEKYLGGEQLSEEEIRHGIRARTIANEIVPMLCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAITGHDEHDKEIERHPNDEDPFSALAFKIMTDPFVGQLIFF
                     RVQRQIGDRLKGVAVPIQIPIGAEEHFQGV"
     gene            complement(1403..1909)
                     /locus_tag="VHV99_02335"
     CDS             complement(1403..1909)
                     /locus_tag="VHV99_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEX3634326.1"
                     /translation="MRFPMRSLIARFAVAVALPLAALSFAATTAAPAHAQQMPAGAKQ
                     PGDFPRAKLTAGMFVIDAAVAANDADREQGLMYRTTLAPNEGMLFVFNENAVHCFWMK
                     NTLIPLSIAFMRADGTVTDIDEMQAETTNNHCPTNNGVYALEMSKGWFSSKGIKPGMK
                     IQGLPAAQ"
     gene            complement(2056..2250)
                     /locus_tag="VHV99_02340"
     CDS             complement(2056..2250)
                     /locus_tag="VHV99_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634327.1"
                     /translation="MNKLIAALVAGLFATAAFAQASAPAAASAAPMAAASSAKKTTKH
                     AHKKSHKKAAAAAAASGASE"
BASE COUNT          408 a          689 c          721 g          500 t
ORIGIN      
        1 cacgccctgg aagtgctcct ccgcgccgat cggaatctgg atcggcacgg cgacgccctt
       61 caggcgatcg ccgatctgcc gctgcacgcg gaagaagatc agctggccga cgaacgggtc
      121 ggtcatgatc ttgaaggcga gcgctgagaa cggatcttcg tcgttcggat gacgttcgat
      181 ctccttgtcg tgctcgtcgt gaccggtgat cgcgggcacg tcgacgggcg acggcagata
      241 gtcgatcacc gcgtcgagca tcgcctgcac gcccttgttc ttgaacgcgc tgccgcacag
      301 catcggcacg atctcgttgg cgatcgtgcg cgcgcggatg ccgtgccgga tttcttcttc
      361 gctgagctgc tcgccgccca ggtacttttc gagcagcgtc tcgttcgcct cggcggcggc
      421 ctcgaccatc ttgtcgtgcc attccttcgc ggtggccgcg agttcagccg gaatgtcgcg
      481 gtactcgaac ttgatgccct ggctggcgtc gtcccagtag atcgccttca tcttcacgag
      541 gtcgaccacg ccctggaagt gatcttccgc gccgaccgga atctggaccg gcaccgcggt
      601 gcccttgagg cgctccgaga tctggcgctg cacgcggaag aagtccgcgc cgacgcggtc
      661 catcttgttg acgaacgcga tgcgcggcac cttgtacttg ttcgcctggc gccacacggt
      721 ttccgattgc ggctgcaccc cgccgaccga gtcgtacacc atgcaggcgc cgtcgagcac
      781 gcgcatcgaa cgttccactt caatcgtgaa gtcgacgtga cccgtggtgt cgatgatgtt
      841 gatccggtgt tcgggatagt tgccggccat gcctttccag aaggcagtgg tggccgccga
      901 cgtgatggtg atgccgcgct cctgctcctg ttccatccag tccatcgtcg cagcaccgtc
      961 gtgaacctcg ccgatcttgt gggtcacgcc tgtgtagaac aggatgcgct cggtggtggt
     1021 ggttttgccg gcgtcgatgt gagcgctaat gccgatattc cggtagcgct cgatgggagt
     1081 cttgcggggc acgtgaacct ccttggaatg gcgcgcctga aacgcttgcc gggcggcagc
     1141 ctgtgctgcc cggggagcct gggtgctgct ttactaagaa tactaggata gcgcgtcggc
     1201 ccgtcttttg caggaatctt gccagccgcc cgtggaggcc tgcaggcatg ggctcaaagc
     1261 gcggcgaagg ccttggcggc gccgcgcatt gcaccgagtc gctggcgctg aatgcaaaaa
     1321 accggtgcct cgtgaaaggc accggttttt ttgaaaagca gccgttgaag aatcgagcgg
     1381 tgtgccgata gtcgacgcgc ttttattgcg cggccggcaa gccctggatc ttcatccccg
     1441 gtttgatgcc tttcgacgag aaccagccct tgctcatttc caacgcatac acgccattgt
     1501 tcgtcgggca atgattgttg gtggtctcgg cctgcatttc gtcgatgtcg gtgaccgtgc
     1561 cgtcggcgcg catgaacgcg atcgacagcg ggatcaacgt gttcttcatc cagaagcaat
     1621 gcacggcgtt ttcgttgaag acgaacaaca tgccttcatt cggcgcgagg gtcgtgcgat
     1681 acatcaagcc ttgttcgcgg tcggcgtcgt tggcggcgac cgccgcgtcg atcacgaaca
     1741 tgcccgcggt cagcttcgcg cgcggaaaat cgccgggctg cttcgcgccg gccggcatct
     1801 gttgcgcatg agcgggggcg gcggtcgtcg cggcgaacga cagcgcggcg agcggcaggg
     1861 caacggcaac agcgaaacgc gcaatcaacg agcgcatggg aaatcgcacg gcatgactcc
     1921 ttgctggaaa ttaacccgcg aatgttacgc gagcgcgtca aaaacaaaaa ggcagatcgc
     1981 ctttcggtga cctgcctttc aacgccgtaa gaacggcaat gaagctagtt cttgactgcg
     2041 ttttttacaa aaaacttact ccgaagcgcc cgaagctgca gctgctgctg ctgccttctt
     2101 gtgcgacttc ttgtgagcgt gcttcgtggt cttcttcgcc gacgaagctg ctgccatcgg
     2161 agctgccgaa gctgctgccg gtgccgaagc ttgtgcgaat gctgccgttg cgaagaggcc
     2221 agcgaccaga gcggcgatca gtttgttcat tttgtaaatc ctcagcttga gtttaattaa
     2281 ccaaatgacc cggttatatg tagagtcatg tcggtaaa
//
LOCUS       DATASH010000080        13464 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2581327,
            whole genome shotgun sequence.
ACCESSION   DATASH010000080 DATASH010000000
VERSION     DATASH010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 13464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13464
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2581327"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..141
                     /locus_tag="VHV99_02345"
     CDS             <1..141
                     /locus_tag="VHV99_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007741995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate ion family chromate transporter"
                     /protein_id="HEX3634328.1"
                     /translation="WTSAVLSELDFAIAVISFFLLTRWKIPPLAIVVLCALAGIAEAS
                     LH"
     gene            complement(200..1303)
                     /locus_tag="VHV99_02350"
     CDS             complement(200..1303)
                     /locus_tag="VHV99_02350"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX3634329.1"
                     /translation="MLLIDNDCVARVLTMRDCIDVQERAFAGLPDGGSIGRPRIDTYV
                     PAARDDSYYRFGSVDGATDGILAVRLKSDVMVWPRDADGRAGSEQKYCVEPGTYCGLV
                     MLFSTENGKPLAMLNDGHLQHMRVGGAAGLGTRLLAREDSQVVGMIGSGGMAETFLEA
                     LVAVRDIRRVRVFSRREVNRTRFAQDMAAKLGIEVEAVGSAEEAVRGADIVTTCTDSM
                     TPVIDADWLEPGMHVVALTPREFDARVASRFDVSIAQGHERLPMAECGRFSQRVSGSP
                     NAFIGGSGDERARLPVSRGERVDTTGWPVYTDVITGRSPGRTDARQITFYYTVGNWGV
                     QFAAVGGHVYREAARLDLGISLPDHYFLQSIRN"
     gene            complement(1354..2853)
                     /gene="tcuA"
                     /locus_tag="VHV99_02355"
     CDS             complement(1354..2853)
                     /gene="tcuA"
                     /locus_tag="VHV99_02355"
                     /inference="COORDINATES: protein motif:HMM:NF006130.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HEX3634330.1"
                     /translation="MTLDELNDIDVLVIGAGNAAANAALAAREGGAKRIVLLESAPEE
                     ARGGNSAFTGGAFRFVYESVDDLLKLAPDIAELDLPNIDFGTYTREQYFDDIGRLTEF
                     RADPELTDVLIGNSYDAALWLKRHGVRFQPALGRQAFRVEGKFRFWGGLACHILGGGT
                     HLVETLHHALKEAGIGVLYRTQAMGLLHGDGRIEGVRAMHEGRQVDIRAKAVVLACGG
                     FESSAEMRARYLGPNWDLAKVRGTRYNTGWGHKMAMEIGAAVTGHWSGAHAVQWDMNA
                     PEYGDLTIGDRFQKHNYPFGILVNAKGERFLDEGLDFHSFTYAKYGYEVLRQPGLFAW
                     QIFDQKVVKLLRDEYRIARITKETANTLEELATKLTGVDPEGFLRTVRAYNEASRPDV
                     SFNPNIHDGLRTRGLAIDKTNWANRLDTPPYEAYGVTTGVTFTFGGLKVSTEAEVLDT
                     SGTPIRGLFAAGEIVGGLYYHNYGSGTGLVAGVVFGRRAGAGAARLVAR"
     gene            complement(2881..4272)
                     /locus_tag="VHV99_02360"
     CDS             complement(2881..4272)
                     /locus_tag="VHV99_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3634331.1"
                     /translation="MNDSLVAARVDRLPNSRMHREWMFLFAIPLFFDSCDINTFASAA
                     PALIRHWHIAINEIALITSASFIGMFFGATLGGLLSDRFGRKRSLMLFVLISSLGSLA
                     TAVVPSVPYLFAARVVTGFGISAGMVTVMTYIAELFPARSRGTWQSWAMVINLCAIPL
                     TNFVARLLVPYGPDGWRWVFVWGALGVAFPLLAKRLPESPRWCARAGRFDEAEAALAA
                     MEARSIAEGNVLSAPEVVVRPPDAREPWMTMFNSVYRRRTLTLCAIWLLQTIGFYGFE
                     AWVPTLLVHHGITIARSLTYFTLINVGAPLGALLAVYPADRFERKHLIALVALLIAVF
                     GLLYGLSFTPVLIVTFGFAVGMLTQTFATLLYSYTPEQFPTGVRNSATGLTYGAGRIA
                     NVANAFVIAAIYTNYGYVTVFMYIAGAWFLTALITLVFGPRTTGRSLETLNPVVDEVD
                     AASLTPLAEYPRR"
     gene            complement(4352..5719)
                     /locus_tag="VHV99_02365"
     CDS             complement(4352..5719)
                     /locus_tag="VHV99_02365"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX3634332.1"
                     /translation="MSIAKSLGERIAAFTSVDVPDGALRWARIGIIDTIGVTLAGAAE
                     PCARMARTVAAPSNGAALVVGTRERMKPSDAALVNGTAAHALDFDDCSNTLGGHPSAP
                     ILPALFALAEEVRASGRRLVTAYVAGFETQARIAHAVNFHHYDKGWHPTATLGVFGAC
                     AASAHLLELDAERTATALSIAASFASGIKANFGTMTKPLHVGHASRNGLIATLLAREG
                     FSAGDAAFEHRQGFFNVYNGAGHYDAEAALAHWADPLDIVEPGIAIKQYPCCGSTHPA
                     IDAMLMLVSEYGLKPDEVESVVSWTHPRRLQHTNRPQPASPLDAKFSVQYCVARALAQ
                     GRVVLSDFEGDAYADPAIRALMSKIVAEPDPLADQQPDHFGAEVVVQLRDGRRVAKRV
                     ARAAGRTSANPLPDARIEAKFLDCANHALTPDASRDALGMLWRIDSMEDMTALTALLE
                     AGLRH"
     gene            complement(5716..7167)
                     /locus_tag="VHV99_02370"
     CDS             complement(5716..7167)
                     /locus_tag="VHV99_02370"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX3634333.1"
                     /translation="MITELITNMTTKVNGDAFMQPASSAGPTAAQLLSEYALSVRFEQ
                     LPADIVRLAKDCLIDAIGAAVYGHEVEAGQAALRYVSHASAGPCAIPGTSRRVAAEAA
                     AFTGGVLVHAAELDSLRQPGAGVHPGASLVPVALAAAQVCGASGRDLLAALVAGIEVL
                     FRIGSATKHSAEARGFHAPGLTGPFGAAIVAGKLAGVDADTLRQALGIAGSLSGGLLE
                     FAKSGDGGLVKRLHLGRAAQSGIVAVQLAQAGFDGPSTVLEGRYGFLKAYCEASDVAR
                     LTEDLGTRYETPTLCLKRYACHIVAHTPVYAAQQLRAAHDIDARRIAAIRIEGAPRLA
                     ANHDIKQPANQVLAQYSVPVCVAAALLHDADDPSTFGVRLLQDQTIRELAQRVTLVAT
                     DATGLAAVTRITLDDGTNYELAQTDFPGCPSTPFTPAQLRTKFMWMTKRLGAAAHTLF
                     ERLDRVEGEADLHWLDAVPALSPDLSLIKGDRG"
     gene            complement(7193..8530)
                     /locus_tag="VHV99_02375"
     CDS             complement(7193..8530)
                     /locus_tag="VHV99_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007580955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3634334.1"
                     /translation="MAATAHPLTLPATPAARRARLRSIIGSSAGNVIEWYDFLAYSIF
                     SIYFSKAFFPGGNQTAQLLNAAAVAAIGYVVRPLGSWAIGALADRQGRRVALSVSVAM
                     MSVGSLIIALTPTYASIGVAAPAVLVCARLLQGFSMGGEAGTSATYLSEMAPTGRRGF
                     FVGFVQVTVVMGQLVALALMLILQRLVLTPTQLEQWGWRIPFAIGGALAVFALYLRRG
                     IAETDAFEREGGANAGMRTQTERGSLLVLLVQHRRQTLWTIGISVGGTVAFYTFTIYL
                     QKFMVNTTGFSRDTATLIALVIYMGFQPLFGLLSDLIGRRPVMMIFGVGGTFLTVPLM
                     HALGATHSALTAFALNLAALGILSGFSSIHWLVKSELFPAKVRALGVGLPFAVVSSVM
                     GGTTEYLALRFKHAGHESWFFYYVSACAAVSLLTYVLMPETRHRSVIDEEARQ"
     gene            complement(8540..9955)
                     /locus_tag="VHV99_02380"
     CDS             complement(8540..9955)
                     /locus_tag="VHV99_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007580957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX3634335.1"
                     /translation="MNQHNDVGTNPYTGGVAAFVAGLRYEAIPDEVRARIKLLILDSL
                     GCAIFGAGLPWSRILADTLQRVDTTPGCHVWGMPLRLSAPHAALVNGTLIQSFELDDV
                     HRVGVLHVGAVTLPAVLAVAELLSADAPMTGRDFLRACVAGYEVGPRVGMCMGPEHIA
                     QGWHSGATVGVFSAAAGAAAALRLDAEQTVHALGIGGTQSAGLMAAQFGAMVKRMHAG
                     RAAQSGLYGALLAQNGFTGIVDVFENPYGGFCSTFSRSLDRFDLTQLVDGLGERFETM
                     RIALKFYSCVGSNHTTLDAIRAMQARHPFGPDDIERVVVHGSRVTVDHVGWKYVPQGL
                     TSAQLNLPYCVATLLLEGDVFVDQFSEDKVADPERMALADRVQVVEDPAITARGSMLR
                     HMVRVEVLLRDGTRLEETVEAQRGSEHAFASEGDIVGKMRKLAAHRIDARQIARIVDW
                     VMHAEDQADASELARLLATVA"
     gene            10101..11030
                     /locus_tag="VHV99_02385"
     CDS             10101..11030
                     /locus_tag="VHV99_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007739423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3634336.1"
                     /translation="MNRFPGVDLDALRAFVAVASHASFNDAALELNLSASALTRRIKR
                     LEEALDTLLFERTTRNVALTSSGELLLPRAQGVLRELDASLELVSEATRIRTGQLTIA
                     CIPTVAKFMLPKIVSSYHRRRPEVRLRLIEANLATVVQRVAIGDAEFGIAFLVNESPE
                     LAIDELLVDPYVLACPADHPLAASEHVTWPELRPWPLIVSGTSSGNRRMLDAALRDID
                     WRPDRLIEIEHLTTSLGLVEAGLGISIIPRCAAPRDAHARIAIRPLVDPTVARTIGLI
                     RRRDAALSPIARDFRLALRRMAPFDSSILHEPA"
     gene            11147..12586
                     /locus_tag="VHV99_02390"
     CDS             11147..12586
                     /locus_tag="VHV99_02390"
                     /inference="COORDINATES: protein motif:HMM:NF014503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634337.1"
                     /translation="MSKARPRLEHMLILLPGITGSVLEKNGKLIWGPAPHALVSLASS
                     MGASLNGLRLQADGVEDDGVRATRIMPYTLIPGISYFDGYTGLHDELHHRFALTEGDA
                     LNPDGEPANFFEFPYDWRRCNRISAKRLKELVDRELPKWRKHSGSPDAGVILLCHSMG
                     GLVGRYYVEVLRGFECVRALVTFGTPHRGAIDAAASLANGFTLAGKNFTALNDALKSF
                     PSAYQLLPRYPSILDSEGNWKRPYEVATREWAIDQTNAIDAFAFHEEIEHASARNRQS
                     QSYTTELIPVYGWGQRTYQSGQLHGDGVLDLQMSLPPGVDPFAFPDGDGRVPRVSAIP
                     IPLDSQPPRWWPVNQRHSTLQNNRTLIANLAQMLLALQGDLRAPARGGTHLATERGIA
                     LDLAPAFSAGTPVDFEACSEAHDLGNLSARIEPVAGGPGIEHQLQHDGDRWHGSVTGL
                     AVGDYRLTIIPEHAHTSPGDYIADLFCVV"
     gene            12981..>13464
                     /locus_tag="VHV99_02395"
     CDS             12981..>13464
                     /locus_tag="VHV99_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634338.1"
                     /translation="MRSHGPHQDQTHAFVVIEQDRIAENENRLGWRQVDWQRGWRSDP
                     DGGQLLLERIRAAHGRICAIECTGFARCTTGREHEILPFADATREAERYLETMTHETT
                     VNVVTLQKLKGFTPYSPNERMVARLPRRRAKASTYVGRHWIFKSLSTFAETHACGYFC
                     V"
BASE COUNT         2581 a         4404 c         4069 g         2410 t
ORIGIN      
        1 tggaccagtg cggttctctc ggaacttgac tttgccatcg cagtcatcag cttcttcttg
       61 ctaacgcgct ggaaaattcc gccgctcgcc atcgttgtgc tctgtgcgct cgccggtatt
      121 gcagaggcaa gcctgcactg atctggcaat tccccttagg aatgggaaag tagtgaatgc
      181 aactcgagcc ctgctccttt caatttctaa tggactgcaa aaaatagtga tccggcaacg
      241 aaatccccag gtctagtcgc gccgcttcgc ggtacacatg cccgcccacc gccgcaaact
      301 gaacgcccca gttacccact gtgtaataga aagtgatctg ccgggcatcc gtgcggccgg
      361 gcgagcgtcc ggtgatcaca tcggtataga ccggccagcc cgtcgtatcc acgcgctccc
      421 cacgcgatac aggcagacgt gcccgttcat ctccacttcc gccgataaac gcattcgggc
      481 tgcccgacac gcgctggctg aagcgtccgc actccgccat cggcagacgt tcatggcctt
      541 gcgcgatgct cacgtcgaag cggcttgcca ccctggcgtc gaattcacgc ggggtgagcg
      601 ccaccacgtg catgccgggt tccagccagt cggcgtcgat cacgggcgtc atgctgtcgg
      661 tgcaggtggt gacgatgtcg gcaccgcgca ccgcctcctc agccgagccg accgcttcga
      721 cttcgatccc aagcttcgcc gccatgtcct gcgcaaaacg cgtgcgattc acctcgcgac
      781 ggctgaagac acgtacgcgc cgaatgtcgc gcaccgccac cagcgcttcg agaaacgttt
      841 ccgccatacc acccgagccg atcataccga cgacctgcga gtcctcgcgc gcaagcagcc
      901 gcgtgccgag ccccgccgcg ccgccgacgc gcatgtgttg caggtggccg tcgttgagca
      961 tcgcgagcgg cttgccgttc tccgtactga acagcatcac cagtccgcaa tacgtgccgg
     1021 gctcgacgca gtacttctgc tcgctgcccg cgcggccgtc ggcgtcgcgc ggccacacca
     1081 tcacatccga cttcagacgc accgccagaa tgccatcggt cgcgccgtcg accgagccga
     1141 agcggtaata gctgtcgtcg cgcgccgccg gcacataggt gtcgatacgc ggacgcccaa
     1201 tcgacccgcc atccggcaag ccggcaaacg cgcgttcctg cacatcgatg caatcgcgca
     1261 tggtgagtac gcgcgccacg cagtcgttat cgataagcaa catcgtagtg agtccctgtg
     1321 cgtccgaatc ggcggtctta attagcgttc gagttagcgc gccacgagcc gcgccgcccc
     1381 tgccccagca cgccgcccaa acaccacgcc ggcgaccagc cccgtgccgc tgccgtagtt
     1441 gtgatagtaa agaccgccga caatctcacc cgccgcgaac aacccccgaa tcggcgtacc
     1501 cgacgtgtcg agcacctcgg cctcggtcga caccttcagt ccaccgaacg tgaaagtcac
     1561 gccggtggtg acgccataag cttcataggg cggcgtgtcc agccggttgg cccagttggt
     1621 tttgtcgatg gcgagcccgc gcgtgcgcag accgtcgtgg atgttcggat tgaacgacac
     1681 atcaggccgc gacgcctcgt tatacgcgcg cacggtgcgc agaaaacctt ccggatcgac
     1741 gcccgtgagt ttcgtcgcca gttcttcgag cgtgttcgcc gtctctttcg tgatgcgcgc
     1801 aatccggtat tcgtcgcgca gcagcttgac gactttctga tcgaagatct gccacgcgaa
     1861 aagacctggc tggcgcaaga cttcataccc gtatttcgcg tacgtgaagc tgtgaaagtc
     1921 gagtccctcg tcgagaaaac gctcgccctt cgcgttcacc agaatcccga acggatagtt
     1981 atgtttctgg aagcggtcgc caatcgtcag atcgccgtac tcgggtgcgt tcatgtccca
     2041 ttgcaccgca tgcgcgcccg accagtgccc cgttaccgcc gcgccgattt ccatcgccat
     2101 cttgtggccc cagccggtgt tgtagcgtgt gccacgcact ttcgcgagat cccagttcgg
     2161 cccgagatag cgcgcccgca tttccgcgct cgactcgaaa ccaccgcatg caagcaccac
     2221 cgctttcgcg cggatgtcga cctgccggcc ctcatgcatg gcacgcacgc cttcgatacg
     2281 cccgtcaccg tgcaacaggc ccattgcctg agtacgataa agcacaccga tgcccgcctc
     2341 ctttaacgca tggtgcagcg tttcgacgag atgcgtgccg ccgcccagaa tgtggcaggc
     2401 cagcccgccc cagaaacgga acttgccttc cacgcgaaac gcctgccgcc ccagcgccgg
     2461 ttgaaaacgc acgccatgcc gcttgagcca caacgccgcg tcgtagctat tgccgatcag
     2521 tacgtcggtc agttccgggt cggcgcgaaa ctccgtgagc cggccgatat cgtcgaaata
     2581 ctgctcgcgc gtgtaggtgc cgaaatcgat attcggcaga tccagttccg cgatatccgg
     2641 cgcgagcttc agcagatcgt cgacggattc gtaaacgaaa cggaatgcgc cgccggtaaa
     2701 cgcggagttg ccgccgcgtg cttcttccgg tgcgctttcg agcagcacga tgcgcttcgc
     2761 gccgccttcg cgcgcggcaa gcgccgcatt ggccgcggcg tttcccgcgc cgattacgag
     2821 cacgtcgata tcgttgagtt cgtcgagggt catcagttgg ctctccagtt tcaatgtgat
     2881 ctagcgtcgt gggtattcgg cgagcggcgt cagcgacgct gcgtccactt catccacgac
     2941 cgggttcagg gtttcgaggc tgcggccggt ggtccgcggg ccgaacacga gtgtgatcaa
     3001 cgcggtcagg aaccatgcgc ccgcgatgta catgaatacg gtcacatagc cgtagttcgt
     3061 gtagatcgcg gcgatcacaa aggcattggc aacgttcgcg atccggcccg cgccatacgt
     3121 gagccccgtt gcgctgttgc gcacgccggt tggaaactgt tcgggcgtgt acgaatagag
     3181 cagcgtcgcg aaggtctgcg tcagcatgcc gaccgcgaat ccgaacgtca cgatcagcac
     3241 cggcgtaaag cttaggccgt acagcaggcc gaatacggcg atcagtagcg cgacgagcgc
     3301 gatcagatgc ttgcgttcga aacggtcggc tggatagacc gcgagcaatg cgccaagcgg
     3361 cgcgcccacg ttgatcagcg tgaaatacgt gaggctgcgc gcaatcgtga tgccgtgatg
     3421 cacgagcaag gtcggcaccc atgcttcaaa gccgtagaag ccgattgtct gcagcagcca
     3481 gatcgcgcag agcgtgagcg tgcggcggcg gtagacgctg ttgaacatgg tcatccacgg
     3541 ttcgcgcgcg tcgggcggcc gcacgacgac ctcgggcgcg ctcagcacgt tcccttccgc
     3601 gatgctcctt gcctccatcg ccgcgagcgc ggcttccgcc tcgtcgaagc gccccgctcg
     3661 cgcgcaccag cgcggcgact cgggaagccg cttcgcaagc aatggaaacg cgacgccaag
     3721 cgcgccccac acgaaaaccc agcgccagcc gtccggtccg tacggcacga gcagccgcgc
     3781 gacgaagttc gtcagcggaa tcgcgcacag attgatgacc atggcccagg attgccaggt
     3841 gccgcgcgag cgcgccggga acagttcggc gatatacgtc atcaccgtca ccatgcccgc
     3901 ggaaatgccg aagccggtga cgacccgcgc cgcgaacaga tacgggacgc tcggcacgac
     3961 cgcggtggcc agcgagccga gcgacgagat cagcacgaac agcatcagcg agcgtttgcg
     4021 cccaaaccga tcggacagca aaccgccgag cgtcgcgccg aagaacatgc cgatgaagct
     4081 cgcggacgtg atcagcgcga tttcgttgat ggcgatgtgc caatgccgga tcagcgcggg
     4141 cgccgccgat gcaaacgtgt tgatatcgca gctatcgaaa aagaggggaa tcgcgaatag
     4201 gaacatccat tcgcgatgca tccgcgagtt cggcaaacgg tcgacgcgcg cggcgacgag
     4261 tgagtcgttc atgcctgtct ccttccggcg gtcgtagccg ccgattgtct tgctctttcg
     4321 caggacgtcc cacgacatcc cgacaactgc gtcagtgccg caaacctgct tcgagtaaag
     4381 cagtcaacgc agtcatgtct tccatgctgt cgatgcgcca gagcatgccg agcgcgtcgc
     4441 ggctcgcatc cggcgtcaag gcatgattcg cgcagtcgag aaacttggct tcgatccgcg
     4501 cgtccggcag cggattggcc gacgtcctgc cggccgcacg cgcgacgcgt ttcgccacac
     4561 gccgcccgtc gcgcaactga acgacgactt ccgcgccgaa atgatccggc tgctggtccg
     4621 ccagaggatc aggctcagca acgatcttcg acatgagcgc gcgaatcgcc ggatcggcgt
     4681 acgcgtcgcc ttcgaagtcg gacagcacca cgcgtccttg cgccagtgcg cgcgccacgc
     4741 agtactgcac gctgaatttc gcgtcgagcg gactggccgg ctgcgggcgg ttcgtgtgct
     4801 gaaggcggcg ggggtgcgtc cacgacacga ctgattcgac ctcgtccggc ttcagaccgt
     4861 attcgctgac gagcatcagc atcgcgtcga tcgcggggtg ggtgctgccg caacacggat
     4921 actgcttgat cgcaatgccg ggttcgacga tgtcgagcgg atcggcccaa tgcgccagcg
     4981 ccgcctcggc gtcgtaatga cccgcgccgt tgtagacgtt gaagaagcct tgacggtgtt
     5041 cgaaggctgc gtcgccggcg ctgaatcctt cgcgcgccag cagcgtcgcg atcaggccgt
     5101 tgcgcgacgc atgaccgaca tgcaagggct tcgtcatggt gccgaaattg gccttgatac
     5161 cggaggcgaa cgacgcggca atcgacaacg cggtcgccgt ccgctctgca tccagctcga
     5221 gcagatgtgc cgacgcggca cacgcgccga atacgccaag cgtcgcggtg ggatgccagc
     5281 ccttgtcgta gtggtgaaag ttgaccgcat gcgcgatgcg agcctgcgtt tcaaatcccg
     5341 ccacgtacgc agtgacgaga cgccggccgc tcgcgcgcac ttcttcggcc agggcgaaga
     5401 gtgccggcag gatcggcgcg gaaggatggc cgcccagcgt attgctgcaa tcgtcgaagt
     5461 cgagcgcatg cgcggccgtg ccgttgacga gcgcggcgtc gctgggcttc atccgctcgc
     5521 gcgtgccgac cacgagtgcc gccccgttcg acggcgccgc cacggtccgc gccatccgcg
     5581 cgcatggctc cgctgcaccc gccagcgtca cgccgatcgt atcgatgatg ccgatgcgcg
     5641 cccagcgcaa cgctccgtcg ggcacgtcga cggaagtgaa cgcggcgatg cgttcgccta
     5701 gcgactttgc gatgctcatc cgcggtctcc ttttataagc gataggtcgg gcgacaatgc
     5761 aggcaccgca tcgagccagt gcagatccgc ttcgccctcc acccgatcca accgctcgaa
     5821 caacgtgtgc gccgcggcac ccagccgctt cgtcatccac atgaacttgg tgcgcaactg
     5881 ggccggtgtg aacggtgtcg acggacaacc cgggaagtcc gtctgcgcca gctcgtaatt
     5941 cgtgccgtca tcgagcgtga tgcgtgtgac ggcggcaagg ccggtggcat cggtcgcgac
     6001 cagcgtcacg cgctgcgcta gctcgcggat cgtctggtct tgcaacaggc gcacgccgaa
     6061 tgtcgacgga tcgtcggcat cgtgcagcag cgccgccgcc acgcaaaccg gcacgctgta
     6121 ttgcgcgagc acctgattgg ccggctgctt gatatcgtga ttcgcggcaa gacgcggcgc
     6181 gccctcgatc cggatcgcgg cgatacgtcg tgcatcgata tcgtgcgcgg cgcgcaattg
     6241 ctgcgcggcg tagaccggcg tgtgtgccac gatgtggcac gcgtagcgct tcaggcacag
     6301 cgtcggcgtt tcatagcgcg tgcctaaatc ctcggtcaaa cgcgccacgt ccgaggcctc
     6361 gcaatacgcc tttagaaaac cgtaacgccc ttcaagcacc gtcgacggtc cgtcgaagcc
     6421 cgcctgcgcc agttgcacgg caacgatgcc cgattgcgcc gcgcgcccca ggtgcagacg
     6481 tttgacgaga ccaccgtcgc cggacttcgc gaattcgagc aatccgccac ttaacgagcc
     6541 ggcaatgccc agcgcctgac gcaacgtatc ggcatcgacg cccgccagtt tgcccgcgac
     6601 aatcgcggcg ccgaacggtc ccgtgagacc cggcgcatga aagccgcgtg cttcggcgga
     6661 gtgcttcgtt gcgctgccga tgcgaaacag cacctcgata ccggcgacga gcgccgccag
     6721 caagtcgcgt cccgacgcgc cgcaaacctg tgcggcggca agtgcaaccg gaaccagcga
     6781 cgcgcccgga tgaacaccgg cgccgggctg ccgcaagctg tcgagctccg ccgcgtgaac
     6841 cagcacgcca cccgtgaacg ccgccgcctc ggccgcgaca cgccggctcg tgccggggat
     6901 cgcgcacggg cccgcggacg catgactcac atagcgcagt gcggcttgac ccgcttccac
     6961 ttcatgtcca tacactgccg cgccgatcgc atcgatcagg cagtctttcg cgagccgcac
     7021 gatgtcggcg ggcaactgct cgaaccgcac gctgagcgcg tactccgaca acaactgcgc
     7081 cgcagtgggc ccagcggatg aagccggctg catgaaagcg tctccattga ccttggtggt
     7141 catattggta atcaactcgg tgatcatctc agtgagcacc tcagtgacca cattactgac
     7201 gcgcttcttc gtcgatcacc gaccgatggc gcgtctcggg catcagcacg tacgtgagca
     7261 gcgacaccgc cgcgcacgcg ctgacgtagt agaagaacca cgattcatga cccgcgtgct
     7321 tgaagcgcaa cgcgagatat tcggtggtgc cgcccatcac cgacgacacg accgcaaacg
     7381 gcaggccgac gccgagcgca cgaaccttcg ccggaaaaag ctccgacttg acgagccagt
     7441 gaatcgacga aaagccactg agaatgccga gcgccgcgag gttcagcgcg aacgccgtga
     7501 gggcgctatg tgtcgcaccg agcgcatgca tcagcggcac cgtcaggaac gtgccgccca
     7561 caccgaagat catcatgacc ggacgccgtc caatcagatc ggacaacagg ccgaagagcg
     7621 gctgaaaacc catgtagatc acgagcgcga tcagcgtcgc cgtgtcgcgg ctgaagcccg
     7681 tggtgttgac catgaatttc tgcaaataga tcgtgaacgt gtagaacgcg accgtgccgc
     7741 cgacgctgat gccgatcgtc cacagcgtct gacgccgatg ctggaccagc agcaccagca
     7801 gactgccccg ctctgtctgg gtccgcatgc cggcattggc gccgccctcg cgctcgaacg
     7861 cgtcggtctc cgcaatgccg cggcgcagat agagcgcaaa gaccgccagc gcgccgccga
     7921 tcgcaaacgg aatgcgccac ccccactgtt cgagttgcgt gggcgtcagc acgagccgtt
     7981 gcaggatgag catcagcgcg agcgccacca gttgccccat caccaccgtc acctggacga
     8041 agccgacaaa gaaaccgcgc cggcccgtcg gcgccatttc actcagatag gtcgcgctcg
     8101 tgcccgcttc gccgcccatg ctgaaacctt gcaggaggcg cgcgcagacc agcacggcgg
     8161 gtgcggccac gccgatcgac gcatacgtcg gcgtcaaggc gatgatcagc gagccgacgc
     8221 tcatcatcgc aaccgatacg ctcaacgcca cgcgccggcc ctgccggtcg gcgagcgcgc
     8281 cgatggccca actgccgagc ggccgcacga cataaccaat tgccgcgacg gccgccgcat
     8341 tcaggagttg cgcggtctga ttgccgcccg ggaaaaaagc cttgctgaaa tagatcgaga
     8401 agatcgaata cgccagaaag tcataccact cgatcacgtt cccagcactg ctgccaataa
     8461 tcgaccgcaa ccgtgcgcgc cgcgcggcgg gcgtggcggg caacgtaagc gggtgcgcgg
     8521 tggcggccac aatggctcct caggcaacgg tcgcgagaag gcgtgcaagc tcgcttgcgt
     8581 ccgcctgatc ttcggcatgc atcacccagt cgacgatgcg cgcgatttgc cgtgcgtcga
     8641 tccgatgcgc ggcgagtttt ctcatcttgc cgacgatatc cccttcgctc gcgaatgcgt
     8701 gttcgctgcc gcgctgtgcc tcgaccgttt cttcgagccg cgtgccgtcg cgcaatagca
     8761 cctcgacgcg caccatatgc cgcagcatcg aaccgcgcgc ggtgatcgcg gggtcctcga
     8821 ccacctgcac gcgatccgcc aacgccatgc gctcagggtc cgcgaccttg tcctcgctga
     8881 actggtcgac gaatacgtcg ccttcgagca gcagtgttgc cacacaatac ggcaggttga
     8941 gctgcgccga ggtcagcccc tgcggtacat atttccagcc cacatggtcg accgttacgc
     9001 gcgacccgtg cacgacgacc cgttcgatat cgtccgggcc gaacggatga cgcgcctgca
     9061 tcgcgcggat cgcgtcgagc gtggtgtgat tgctgcccac gcacgagtag aacttcagcg
     9121 cgatgcgcat ggtctcgaag cgctcgccga ggccatccac gagttgcgtc agatcgaagc
     9181 ggtccagcga gcgcgagaac gtactgcaaa atcctccgta cgggttctcg aatacgtcga
     9241 cgataccggt gaaaccgttt tgcgcgagca acgcgccgta caagccgctt tgcgcggcgc
     9301 ggcccgcatg catgcgtttg accattgcgc cgaactgcgc ggccatcagc cccgccgact
     9361 gcgtgccgcc aatgccgagc gcgtgcaccg tctgctccgc atcgagccgc agcgcggctg
     9421 ccgcgccggc ggcagccgag aatacgccga ccgttgcccc tgaatgccag ccttgcgcga
     9481 tgtgctccgg tcccatgcac atgcccacgc gcggaccaac ctcgtagccc gccacgcagg
     9541 cgcgcaggaa gtcgcggcct gtcatcggcg catcggcgga cagcaactcg gcaaccgcga
     9601 gcacggccgg caaggtcacc gcgccgacat gcagcacgcc gacgcgatgg acgtcgtcga
     9661 gttcgaaact ctggatcaag gtgccattga cgagcgccgc gtgtggcgcg gacaaccgca
     9721 acggcatgcc ccacacgtgg caacccggcg tggtatcgac gcgctgcagg gtatccgcaa
     9781 gaatgcggct ccatggcaac ccggcgccaa agatcgcgca gccgagcgaa tcgaggatca
     9841 gcagtttgat gcgcgcgcgc acttcgtccg gtatcgcctc ataccgcaag cccgcgacga
     9901 atgcggccac gccgccggta taagggtttg tgcctacgtc gttgtgctgg ttcatcgatg
     9961 tgggtgccgt ctcgggtgta atcgcgcgga tcacgttgca cggaaatgtc gtgccttcag
    10021 tatccgcaca ccggacgcgt ttgtgaattg cacgcgacgt gattattatt gcgtcacatg
    10081 caagagtcag cggagtgccg atgaaccgtt ttccgggagt cgatctggac gcattgcgag
    10141 cgtttgtcgc ggtcgcgtcg cacgcatcgt tcaacgacgc agcgcttgaa ctgaacctct
    10201 ccgcttccgc gttgacgcgg cgtatcaaac ggctcgaaga agcgctcgat accttgctgt
    10261 tcgaacgcac cacgcgcaac gtcgcgctca ccagttccgg cgaattgctg ttgccgcgcg
    10321 cacaaggcgt gctgcgtgag ctcgatgcat cgctcgaact ggtgagcgaa gcgacgcgta
    10381 ttcgcactgg ccagttgacg atcgcgtgta ttccgacggt cgccaaattc atgctgccga
    10441 aaatcgtcag cagttatcac aggcggcgtc ctgaagtacg gctcaggctc atcgaggcca
    10501 acctcgcgac ggtcgtgcaa cgtgtcgcga tcggcgacgc ggagttcggc attgcgtttc
    10561 tcgtgaacga atcgcctgag cttgcaatcg acgaactgct ggttgatccg tacgtgctgg
    10621 catgtcccgc cgatcatccg ctcgccgcga gcgaacatgt gacgtggccg gagttgcggc
    10681 catggccgtt gatcgtctcg ggcacgtcga gcggcaaccg gcgcatgctc gacgccgccc
    10741 tcagggacat cgactggcgc cccgaccggc tgatcgagat cgaacatctg accacttcac
    10801 tagggcttgt tgaagcgggt ctcgggattt ccatcattcc gcgctgcgcg gcgccgcgcg
    10861 atgcgcatgc gcgcatcgca atccggccgc tggtggatcc gacggtggcg cgaaccatcg
    10921 gtttgattcg ccggcgcgat gccgcgctct cgccgatcgc gcgcgacttt cgtctcgcgc
    10981 tgcgcaggat ggcgccattc gattcgtcga tcctccacga gcctgcctga gcgtagcgga
    11041 ggcgttgcgc cgcacctcat tgcgcgtgac ctgtatcgga gatattctcg accgtgcttg
    11101 cagcgcttta gggaagagcc ggattcatat caggaaagga aatctcatga gcaaggcgcg
    11161 gccgcgattg gaacatatgc tgattttgct gcccggcatc acgggcagcg tgctggaaaa
    11221 aaacggcaag ctgatatggg gtcccgcgcc gcacgcgtta gtgagccttg ctagctcaat
    11281 gggcgcttcg ctaaatggcc tgcggctcca ggccgacggc gtcgaagatg acggcgtccg
    11341 cgccactcgg atcatgcctt acacgttaat cccgggaatt tcctatttcg acggttacac
    11401 gggcttacac gacgaactac atcaccggtt tgcgctgact gaaggcgatg ccctcaaccc
    11461 tgacggcgaa cccgcgaatt tttttgagtt cccttacgac tggcgacgtt gcaatcgcat
    11521 ctcggcaaag cgcctgaagg agcttgttga tcgtgaactg ccgaaatggc gcaaacattc
    11581 gggttccccc gacgcaggcg tcatcttgct ctgccacagc atgggagggc ttgtggggcg
    11641 gtattacgtc gaggtgttga gagggttcga gtgcgtgcgt gcgcttgtca ctttcggtac
    11701 accgcatcgt ggcgctattg acgcggcagc ttccctcgca aacggcttta cgctggccgg
    11761 caaaaatttc acggcgttaa atgacgcact gaagagtttt ccatctgcgt accagttact
    11821 accgcggtat ccgtcaattc tggattccga aggaaactgg aaaaggccat atgaagtcgc
    11881 tacccgcgag tgggctatcg atcagaccaa tgccatcgac gccttcgctt tccacgagga
    11941 gatagagcat gcctctgcgc gcaatcgaca gagtcagagc tataccaccg aactgatccc
    12001 ggtttacggt tgggggcaac gcacctatca atccggtcaa ctacacggtg atggggttct
    12061 ggatttgcag atgagcttac cacccggagt cgacccattt gcctttccgg acggcgatgg
    12121 cagagtgcct cgcgtatccg cgattccgat ccctctggat tctcaaccgc cgcgctggtg
    12181 gccggtcaat caacgacact caaccttgca gaataatcga acgctaatcg caaaccttgc
    12241 gcagatgctt ctcgcactgc aaggagatct gcgagcgccc gcccgcggcg ggacgcacct
    12301 cgcgaccgag cgtggcatcg cgttggatct tgctccggct ttttccgccg gcacgccggt
    12361 cgacttcgag gcatgctctg aggcgcacga tctgggcaat ctgagtgccc gcatcgagcc
    12421 tgtcgctggc ggaccaggca tagagcatca gctgcaacac gacggggatc gctggcacgg
    12481 tagcgttacc ggacttgccg tgggcgacta ccggctgacg attatccctg agcatgcgca
    12541 tacgagccct ggcgactata tagctgacct tttttgcgtc gtctaacgtg tccgatgaac
    12601 tcaaccattg attcatggcg tccgtggcga aagcgcagcc ccgagttatt ggcgaggtat
    12661 gtagtgcgcg aggccgccgc caaggcggtt gaggacgcct ggcaggcacg gggtaataag
    12721 ggatcctttt tttcgaccga tcccggcaca ccgacctttc acgatctttt gcagcaacac
    12781 aggcacctgg aaattgcacg catattctat gagcgactga accgagttat atcgaagggg
    12841 atccttccga cgccgaacaa ctgattcgag aacccggcaa actactcgca aatggtggcg
    12901 gcaatggtac atgcattgat ctggcgctcc tgtttgccgg gcttgcggtt gagattggtt
    12961 tgctcccgct cgtggtgctc atgcgttccc atggaccaca tcaggaccag acgcatgctt
    13021 tcgtcgtcat cgagcaagat cgaatcgcgg agaatgaaaa tcggctcgga tggcgccagg
    13081 ttgactggca acgagggtgg cgctcagacc ctgatggcgg tcaacttctg ctcgaaagaa
    13141 tccgcgctgc ccacggaaga atatgcgcca tcgagtgcac cggatttgcg cgctgcacga
    13201 cgggccgcga gcacgagatc ttgccctttg cggatgctac gcgggaagcg gagcgctacc
    13261 tggagacgat gacgcatgaa acaacggtca acgtagtcac gcttcaaaag ctcaaaggat
    13321 tcacgcctta ctctccaaac gaacgcatgg tcgcgcggct accgcggaga cgagcaaagg
    13381 cctcgacgta tgtgggtcgc cattggatat tcaagtccct cagtacgttt gccgaaacgc
    13441 atgcttgcgg gtatttttgc gtgg
//
LOCUS       DATASH010000081        12752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2186009,
            whole genome shotgun sequence.
ACCESSION   DATASH010000081 DATASH010000000
VERSION     DATASH010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 12752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12752
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2186009"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VHV99_02400"
     CDS             <1..498
                     /locus_tag="VHV99_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX3634339.1"
                     /translation="PENGPQIAELCTATIRAVAQGVVGIAFIQMLLIGIGFVVMGIPG
                     AGLLELAVLLLGIMQLPATLITIPVIVFVIATQGVTTMTVIFSVYVFVAGLADNVLKP
                     LLLGRGVAVPMPVVLIGALGGMVTGGVIGLFIGPVMLSVGYQLFWRWVKDQAKPERLK
                     ETTHT"
     gene            498..2057
                     /locus_tag="VHV99_02405"
     CDS             498..2057
                     /locus_tag="VHV99_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012405644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX3634340.1"
                     /translation="MMPGPAPLSARPYWLLLAAGCVSGCMRVGPDFSPQHERWTEHWS
                     SASIGQVTQQLPQPDMRQWWQVFADRNLESLIEQADANNSDVQVAGLRVLEARAQLGI
                     ALAGRYPQVQQANADVLYAARKRSDGFNPRSGGYWQYGAGLSVGWELDFWGRFSRAIE
                     SADATFLAAQANRDAALVLLHAQVADTYFTLRTAEARLRIARENAQLQKRSYEIAQKL
                     FKSGETDELDLQQAKTQYLGTLSSVPDLESQIVLASHALAVLMGRPPGPLPELDVEPG
                     RNGVIPLVDHAVLQDVPADLLLRRPDVRAAEYQMAAQSALIGVAKADLYPSVSLLGTL
                     AWSASSLTGSPNTLGLLVGPSITWNVFDHGRITNNVRVQDARLQQLTVSYQSTVREAA
                     READDAATTLIAALQRDAILSDAQGAAGRSLKLANAIYREGYSDFQRVLDAQRALFAQ
                     QDAYIVNRSNAAGSLIALYKALGGGWYSKQPLVEPATRLQMQQRTDWGDLLNEPDAAS
                     VTASPSADSSR"
     gene            2054..3181
                     /locus_tag="VHV99_02410"
     CDS             2054..3181
                     /locus_tag="VHV99_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007733224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEX3634341.1"
                     /translation="MSDKPEPIAAPPPKPAAPAADPSRKATKGVVCLIVVSLIWYLLA
                     DRFTPYTQQARVQAYVVAVSAETSGRVTRVLVHDNQEVAAGDLLFEVDGESYRIAVDR
                     ARADLESTRRQIGAGTAGIDSALASLRAAIANEIKARQDSERLERLYRDDEGTVSLRR
                     LEVARATHEQAESQVAAARAEVERAREQQGGIGAENAQLRAAAASVAKAELDLANTQV
                     KARTGGVITDLRTEVGQFAAAGNPVMTLIAIRDVWVSADMTENNMGHLKPGTPVAIAL
                     DALPGEVFKGLVRSIGYGVSVGQSAPPGGLPTVQNSRDWLRPAQRFPVVVEFDQDERA
                     RLRNIRVGGQAEVMAFPDKGNPLNPLGRVFLHVMSWVSYLY"
     gene            3191..4204
                     /locus_tag="VHV99_02415"
     CDS             3191..4204
                     /locus_tag="VHV99_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2955 domain-containing protein"
                     /protein_id="HEX3634342.1"
                     /translation="MENRLWLSGRRALRVAIGTALCLAISFALDLSIPVIAPVFIVFL
                     LAAQNRPLPLKAGLAFALVVMLTTGSGLILVPLLRYYALAGVVLVGLMLFLAFRYGLR
                     GGNNLVSTFLVAGLTMISAAGTANLELAVTVVGALAKGLLLAVLVSVLTHRLFPEPAS
                     TRREPTAQVMSNGEAGRIALRAALVVMPVYLLAMTDPASYMPIIMKSVSLGRQSCTAT
                     ARSAARDLIGATLLGGLLAVVFWFALRLFVHLWMFFLWMLLFGLLVGRKMYGISPTRY
                     SPGFWLNSLVTMIILLGQSVQDSAAGKDVYTAFAVRMGLFVAVTVYACLMLLVLELSG
                     RAR"
     gene            4318..4698
                     /locus_tag="VHV99_02420"
     CDS             4318..4698
                     /locus_tag="VHV99_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012405641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEX3634343.1"
                     /translation="MSNARSEAAVRLRAGDILRESGRTLWYLRSILMGLLLLFVLLAI
                     AMYYMGDPVETVKRTPSSMGETFYFCAITALTIGYGDVVPTSTFGRIDAVLLGVAGVM
                     FTGLIVAAAVRGVQEAARDVSVQD"
     gene            5083..7836
                     /locus_tag="VHV99_02425"
     CDS             5083..7836
                     /locus_tag="VHV99_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylase"
                     /protein_id="HEX3634344.1"
                     /translation="MESAARLVPEFGLNQFFSAAEARVDHWRSLNRIVRAMATQSQQD
                     TVGLRDQALEVLNELAPLEDLNGYPGPYLMAQVHERLAANEWDTLSRLVQRISAALMS
                     NSYRDDKEAWKAEEEGELRLPDFLPPSIGHGQSRKPYFETLVVAAGERSTWPDIRDGF
                     HRLRRNDDGFVYEMVVVGSFEDAILATIFNYNLQSVVIVDGFSFASQYTSAALRDIIV
                     RNTPVGTGAGMDDMGSALARLVRGLRPELDVFLSTDRDVAQLAGSDAAAPLRRIFYGA
                     EEPMEIHLAILDGIRDRYETPYFDNLKHYAARPIGTFHALPVARGKSIFKSNWIRDMG
                     EFYGANLFLAESSATTGGLDSLLEPTGNIKASQDKAARALGGDRTFFVTNGTSTANKI
                     VHQALLTPGDIVLIDRDCHKSHHYGIVLAGAQPLYIDAYPLTQYSMYGSLAIRPIKQA
                     LLALKAEGKLDRARMLVLTNCTFDGHVANVEQTMLECLAIKPDLIFLWDEAWFGYARF
                     SPLLRMRTAMGGAAKLRELMRDPAYRDKYESFRQSSADIGPTDSRLLDMQLLPDPDRA
                     SIRVYETDSIHKSMSALRQASIIVVADEAFHTVEAQFKEAFFTHTSTSPNLQIIASID
                     VARRQMELEGYELVQRAIQLSIEIRREINNHPLISRYFRAATPAEMIPEAYRTSGFAD
                     YGMAGKTVSDATRALRDDEFFLDPTRITLLCGNAGYDGTRFKGLLAESYDIQINKTSR
                     NSVLVQININNTRSDMAHLIKALADISRALERELDQGGESARAGFEARVKALVEDVPE
                     LPNFSRFHNALRENSESATSEGDMRAAFYLAYNPEACEYIRLNDERIDDRLKNGPELV
                     SAKFVIPYPPGFPIMVPGQVITPETIAFMRKLDVKEIHGYNAARGLELLKPEALDALR
                     QRP"
     gene            8104..9789
                     /gene="aspT"
                     /locus_tag="VHV99_02430"
     CDS             8104..9789
                     /gene="aspT"
                     /locus_tag="VHV99_02430"
                     /inference="COORDINATES: protein motif:HMM:TIGR03802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0070906 - aspartate:alanine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-alanine antiporter"
                     /protein_id="HEX3634345.1"
                     /translation="MNWLTKSLQASPELAMFLAVGIGYWVGGIRFGKFSLGTVTASLI
                     VALGIGQLHVEVSRELRWGLFLLFLFANGYAAGPQFFQALKEDGVKPMLLSVTVMVVG
                     LGTAWAMARLFKLDPGTAAGIFCGGLTQSAAIGTATDAIMNLPLPLEERKLLANHVAV
                     ADALTYLFGAIGMILFVSSLAPRLLRVDLREEAAALERKFGIRRDTAGVFAAHQRFAV
                     RSYRLSSSGFAGKRVDEAERSEAPMRYFIEHILRDGSAIEVRPETVLQANDTVTLYGQ
                     MPALVKIGPRFGTELHDPELLQFPIEILKATVTNKALCGVPLEALMRAPEFDFRSIGL
                     RSITRGTQQIPIGTETVLNRGDVLELVGPQRAVERVTAEIGYALRPNHASSLSILGLG
                     ILCGALVGLPYVMLGNIRLTLSVSVGTLVAGLLLGWLRSLRPTLGDIPQPALDLMINF
                     GLAAFVACSGLQAGPEFVRAVKELGLGILVAGAVVTLLPQAVALLVGRYVLRMNPLML
                     LGALAGAQTFTAALAAVQDKAGSRIPVLGYTVPYATSNILLTTGGSIMVALMA"
     gene            10244..12424
                     /locus_tag="VHV99_02435"
     CDS             10244..12424
                     /locus_tag="VHV99_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008352855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX3634346.1"
                     /translation="MAAGLQNLVANAPASTAQAASSPSAASQADQARALDTLIDSLEN
                     DAQRKALVSHLKGLRAASRSAAPEAPAAAAQPNSDLLGAIASGIASVETDLSEGRTPL
                     HYWAARSSAALGDLQAIGAGKNGESFGEVLLWMTATLACWGACACILIYLQRRIVARY
                     GWKVALEPNPSSVELLIFAVWRTAPWIVAFVAVVVFVQRVLDTLGSTLGLVIAYAIVA
                     GSIFSAICMITLSIFNTAHRRVALRQLLTQSRWPLFVIGASGALGDATANPDVIGLLG
                     ANLAGVVSASANVTAAVLIGYFALAFRRPVTHLIRNRPYAQRRDHKLATEALDILASL
                     WHIPILVIAIASVVAIIDARGSEESVLQPSLLSALLLVLTLFMSALLMHVTRRRDTRI
                     HRRAPHLMRLLRFASTVVVFLTWLAFIRFELDLWSGPIARTIKHSAMTPGFRHALVVV
                     IATVFGAWFIWISCDTIILKAIGPSSSRNKALDPGVRARTMLPLVRNAVFATVASLAA
                     VVAAASLGINLTPLLAGAGVIGLAVGFGAKALVTDLITGLFIIVEETISVGDWIEVGS
                     DHAGTVMDLTIRTVRLRDGQGAVHTIPFSQIKIVKNLSRDFANAVFEVRVPFHADMED
                     VTRMILEVSADLFADFNLRKEILGPVEVWGLDRFEANWLVVKAQIKTRPLQQWSVARA
                     FNAGLKRKMDEAGIEIPVPQMLLNTSGGRGPSTQSDDATRNESR"
BASE COUNT         2320 a         3807 c         4029 g         2596 t
ORIGIN      
        1 ccggagaacg gtccgcagat tgccgaactt tgcactgcca cgattcgcgc ggtcgcccag
       61 ggcgtggtcg gcatcgcgtt tattcagatg ctgttgatcg gtatcggctt cgtcgtcatg
      121 ggtattccgg gtgcgggcct gcttgagctc gcggtgctgc tgctcggcat catgcagttg
      181 ccggccaccc tgatcacgat tccggtgatt gtcttcgtga tcgccacgca aggcgtgacc
      241 accatgacgg tcatcttctc cgtgtatgtg ttcgtcgcgg ggctggccga caatgtcctc
      301 aagccgctgc tgctgggccg cggggttgcc gtacccatgc cggtggtgct gatcggcgcg
      361 ctgggaggca tggtgacggg tggcgtcatc ggcctgttca tcggtccggt gatgctttcg
      421 gtgggctatc agttgttctg gcgatgggtc aaagaccagg cgaagccgga gcgcctgaaa
      481 gaaaccacac acacctgatg atgccggggc cggcgccact atccgcgcgc ccatactggc
      541 tgctgctggc ggcgggatgc gtgagcgggt gcatgcgtgt gggccccgac ttttccccgc
      601 agcatgagcg ctggacggaa cactggagca gcgcgtcgat cgggcaggtg acgcagcagc
      661 taccacagcc tgacatgcgg caatggtggc aagtattcgc ggaccgcaac ctcgagagcc
      721 tgatcgaaca agccgatgcc aacaacagcg acgtgcaggt tgccggcctt cgcgtgctgg
      781 aggctcgtgc gcaactcggc atcgcgctcg cgggacgtta tcctcaggtt cagcaagcca
      841 atgccgatgt gctttacgcg gcgcgcaagc gctcggatgg cttcaatccc cgctcgggtg
      901 gttactggca gtacggagcg ggcctgagcg tcggatggga actcgacttc tgggggcggt
      961 tcagccgcgc gatcgagtcg gccgacgcca ccttccttgc cgcgcaagcc aaccgcgatg
     1021 ccgcgctggt cctgctgcac gcacaggttg ccgataccta cttcacgttg cgcacggccg
     1081 aggcgcgcct gcgcatcgcg cgcgagaacg cgcagttgca aaagcgcagc tacgagatcg
     1141 cgcaaaagct cttcaagagc ggtgagaccg atgaactgga tcttcagcag gcaaagacgc
     1201 agtatctggg cacactgagc agcgttcccg atcttgaaag tcagatcgtg cttgcaagtc
     1261 acgcactggc cgtcctgatg gggcggccac ccggtccatt gccggaactc gacgtggagc
     1321 ccggcaggaa cggggtgatc ccgctcgtcg atcacgccgt gctgcaggac gtgccggcgg
     1381 atctgctcct gcgccgcccg gacgttcgcg ccgccgagta tcagatggcc gcgcagtccg
     1441 cgctgatcgg cgtggcaaaa gccgatctct atccgtccgt ctcgttgctg ggcacgctcg
     1501 cctggagcgc gagttcgctg accggctcgc cgaacacgct gggcttgctg gttggcccaa
     1561 gcatcacatg gaacgtgttc gatcacggca ggataaccaa caacgtgcgc gtgcaggacg
     1621 cccgactcca acagttgacg gtctcgtatc agagcaccgt gcgggaagct gcccgtgagg
     1681 ccgacgatgc cgccaccaca ctgatcgcgg cgttgcagcg ggacgccatc ctgagcgacg
     1741 cgcaaggggc cgcgggacgc tcgctcaagc tcgctaatgc gatttaccgg gaagggtact
     1801 cggacttcca gcgggtcctc gacgcgcagc gcgcactgtt cgcgcaacag gacgcctaca
     1861 tcgtcaaccg tagcaacgcg gcgggtagtc tgattgcgct ttacaaggct ctcggcgggg
     1921 gctggtattc gaaacagccg ctcgtcgaac cagcaacacg cctgcagatg cagcaacgca
     1981 ccgactgggg cgacctgcta aatgaaccgg atgcggcatc ggtcaccgcc agcccgtccg
     2041 cggactcgtc ccgatgagcg acaagcccga gcccatcgcc gcgccgccgc cgaagccggc
     2101 agcgcctgcc gccgacccgt cgcgtaaagc gacgaagggg gttgtctgtc tgatcgtcgt
     2161 cagcctgatc tggtatctcc tcgccgatcg ctttacccct tacacgcaac aggcgcgcgt
     2221 gcaggcctac gttgtcgctg tctccgcgga aacatccggg cgggtcaccc gtgtcctcgt
     2281 ccatgacaat caggaagtcg cggcgggcga cctgctcttc gaagtcgatg gcgagtcgta
     2341 ccggatcgct gtcgatcgtg cgcgggccga cctcgagtcg acacgtcggc aaatcggcgc
     2401 cggcaccgcc gggatcgatt cggcgctggc atcgttgcgc gcggcaatcg ccaatgagat
     2461 caaggcacgc caggacagcg agcgcctcga aaggctgtac cgcgatgacg agggtaccgt
     2521 gtcgctacga cgcctcgaag tggcgcgtgc gacgcacgag caggccgaaa gtcaggttgc
     2581 cgcagcgcgc gccgaagtgg agcgggctcg cgagcagcag ggcggcattg gcgcagagaa
     2641 tgcgcaattg cgcgctgctg ccgcttcggt ggcaaaggct gaactcgacc tggcgaacac
     2701 gcaggtgaag gcgcgcaccg gcggcgtgat cacggatctg cggacagaag tcggccagtt
     2761 tgctgcggcg ggcaacccgg tcatgacgct gattgccatc cgtgacgtat gggtcagcgc
     2821 ggatatgacc gaaaacaaca tggggcatct gaaacccggc acgcccgtgg cgattgcgct
     2881 ggatgcgctg cccggcgaag tgttcaaggg actggtgcgc agtatcggat atggcgtgag
     2941 cgtggggcag agcgcgccgc ccggtggtct tccgactgtg cagaacagcc gcgactggtt
     3001 gcgccctgcg cagcgctttc cggtcgtcgt cgagttcgat caggacgaga gggcgcggct
     3061 gcgcaacatt cgtgtcggtg ggcaggccga ggtgatggcg tttccggaca agggcaatcc
     3121 gctcaatccg ctgggtcgtg tgtttctgca cgtgatgagc tgggtctctt acctgtactg
     3181 aacgacactc atggaaaaca ggctctggct ttccggtcga cgcgcgctgc gggttgccat
     3241 agggacggcg ctttgcctcg cgatcagttt tgcgctggac ctgtcgatac ccgtgatcgc
     3301 gccggttttt atcgtctttc tgctcgctgc gcagaaccgg ccgctgccgt tgaaagccgg
     3361 cctggctttc gcgctcgtcg tgatgttgac gacgggcagc ggcctgattc ttgtcccgct
     3421 gcttcgctat tacgcgctcg ctggggtggt gcttgtcggt ctgatgcttt tcctcgcatt
     3481 tcgctacgga ttgcgcggcg ggaacaacct ggtttcgacg tttctggtcg ccggactgac
     3541 gatgatctcc gcggcgggaa ccgcaaacct cgaactggcc gtgaccgtgg tgggcgcgct
     3601 cgcgaaaggg cttttacttg ccgtgttggt gtccgtattg acgcacaggc tatttcccga
     3661 gcccgcgagc acgcgacgtg agcccaccgc tcaggtcatg tccaacggcg aggccgggcg
     3721 catcgcgctg cgcgccgcgc tcgtggtcat gccggtgtat ctgctcgcca tgaccgatcc
     3781 agcgagctac atgcccatca tcatgaagtc ggtcagtctt ggcagacaaa gttgcaccgc
     3841 gacggctcgc agtgcagcgc gcgatctgat cggggccacg ctgctcggcg ggctgcttgc
     3901 cgtcgtgttc tggtttgcac tcaggctctt tgtgcacctg tggatgttct ttctgtggat
     3961 gctgctgttc ggcctgctag tggggcgcaa gatgtacggc atcagtccta cccggtactc
     4021 accgggcttc tggctgaaca gcctggtcac gatgatcatt ctgcttggcc agtcggtaca
     4081 ggacagcgca gctggaaaag atgtctacac ggcgttcgcg gtgcgcatgg ggctgtttgt
     4141 ggcagtcacg gtctacgcgt gtctgatgtt actggttctg gagctatccg gccgcgcgcg
     4201 atgatactca acgcacgcgg cctccagcgc atggatggcc attcattcgg gtgttgaacc
     4261 caaagccggg tgacattggg gccacgcacg ccacatgcaa tgaaggaggt acctgcaatg
     4321 agcaatgcga gaagcgaagc tgccgtccgc ctgcgggcag gcgacatctt gcgggaatca
     4381 ggcaggacgc tctggtatct gcggtcgatc ctgatgggcc tgctgctcct gttcgtgcta
     4441 ctggcgatcg ccatgtatta catgggagac cccgtggaga cggtgaagcg aacgccttca
     4501 tcgatggggg agactttcta cttctgtgcg atcacggcgc tgaccattgg ctacggcgac
     4561 gtggttccca cttcgacgtt cggccggatc gacgcggtcc tgctaggggt ggccggcgtg
     4621 atgttcaccg ggctaatcgt tgctgcggcc gtccgtggcg tgcaggaagc tgcgcgcgac
     4681 gtcagtgttc aggattaatt gggtcgtgat gccggtggac gaatcgccgt gacgctcggg
     4741 aaatcgatgc gcacgcccag ttctatcgat caccctccac acgagtttct ggatcggttt
     4801 catgccaatt tgcggctgta tcacggtagg aaagcggcgc tgcagtcgct gggattagct
     4861 cgctagtgcg tcccccgttt ttcgaaacgc catttgaaca ggacgcctgc cgacatcgca
     4921 gtctgttcag cccgttgcgt gcataagacc aaaggccgat ttacgcgttg gcggatagtg
     4981 gtcatcgtgg tgaggtgaag actaactcag gggcgctcac tgctcccggc accacctgaa
     5041 cgacacgctg tcaccgatca agaaacattc aggagactcg acatggaatc cgccgccagg
     5101 cttgtgccgg agttcggcct caaccagttc ttctcggctg cggaggcgcg ggtcgatcac
     5161 tggcgcagcc tgaaccggat cgtgcgcgcg atggcgaccc agtcgcaaca ggataccgtc
     5221 ggcttgcgcg atcaggcact ggaggtattg aatgagctgg cgcccctgga agatctgaac
     5281 ggctacccgg ggccctatct gatggcgcag gtgcatgaac ggctcgcggc gaacgagtgg
     5341 gataccctgt cccgtctcgt gcagcgaatc agtgcggcgt tgatgtccaa tagctaccgc
     5401 gatgacaagg aagcgtggaa agcggaagag gagggtgagt tgcggttgcc agatttcctt
     5461 cccccctcca tcggccacgg acaaagtcgt aagccctatt tcgaaacgct cgtggtcgct
     5521 gccggggagc gttctacctg gcctgatatc cgcgacggct ttcaccgtct acgtcgcaac
     5581 gacgacggct tcgtctacga aatggttgtg gtcggcagct tcgaggacgc gatactcgct
     5641 acgattttca actacaacct gcaatcggtg gtgattgtcg acggctttag ctttgcctcg
     5701 caatatacgt ctgccgcatt gcgcgacatc attgtgcgca atacgcctgt gggaacgggc
     5761 gcagggatgg acgacatggg ttcggcgctt gctcgcctgg tgcgggggct gcgccctgaa
     5821 ctcgatgtat ttctctccac cgaccgcgac gtagctcagt tggcgggctc ggatgccgcc
     5881 gcgcctctgc gccggatctt ctacggcgcg gaagaaccga tggagattca tctggccatt
     5941 ctggacggca tcagggaccg ttatgaaacg ccgtacttcg acaacctcaa gcactacgcc
     6001 gcgcgaccta tcggaacctt tcacgcgctg ccggttgcga gaggcaagtc gatcttcaaa
     6061 tcgaactgga tccgcgatat gggcgagttc tatggcgcga acctgttcct cgccgaatca
     6121 tcggccacca cgggcgggct ggacagtctg ctagaaccca ccggcaacat taaggcatcc
     6181 caagacaagg ctgcgcgtgc attgggtgga gaccgcacct tcttcgtgac aaacggcacc
     6241 tccactgcca acaagatcgt tcatcaggcg ctgctgacgc cgggcgatat agtgctgatc
     6301 gatcgcgact gccacaagtc gcatcactac ggcatcgtgc tggccggtgc acagcccctc
     6361 tatattgacg cctacccgct cacccagtac tcgatgtatg gcagtctggc gatacggccc
     6421 atcaagcagg cgctcctcgc gctgaaggct gagggcaagc ttgatcgggc caggatgctg
     6481 gtgctgacca attgcacctt cgatggtcac gttgccaatg tcgaacagac catgctcgag
     6541 tgtctggcca tcaagcccga cctgatcttc ctctgggacg aggcctggtt tggctatgcc
     6601 cgcttctcgc cgctgctgcg catgcgtacg gcgatgggtg gcgccgcgaa actgcgcgag
     6661 ctgatgcgcg atcccgcgta tcgcgacaaa tacgaatcct tcaggcagtc ctccgccgac
     6721 atcgggccga ccgactcccg gctgcttgac atgcagcttc tgcctgaccc cgatcgagca
     6781 agcattcgcg tctatgagac cgactcgatc cacaagtcga tgtcggcttt gcgccaggcc
     6841 tcgatcatcg tagtggccga cgaggcgttt cataccgtcg aggcccagtt caaagaggct
     6901 ttctttaccc atacttcgac ttcgccgaac ctgcagatca tcgcctcgat cgacgtggcc
     6961 cggcggcaga tggagctgga aggatacgaa ctggtacaac gtgccattca actgtcgatc
     7021 gagatccgca gggaaatcaa caatcatccg ctgatttccc gctatttccg tgcggccacc
     7081 ccggcggaga tgattcccga ggcgtatcgg acgtccggct tcgcggatta tggaatggcc
     7141 ggaaagaccg tctccgatgc cacgcgggcc ctgcgcgatg acgagttctt tctcgatccc
     7201 acccgcatca cgctgctttg cggaaacgcg ggttacgacg gtacccgttt caagggattg
     7261 ctggccgagt cttatgacat ccagatcaac aagacctcgc gcaacagtgt gctggtgcag
     7321 atcaatatca acaatacccg cagcgacatg gcgcacctga tcaaggcgct tgccgacatc
     7381 tcacgggcgc tggaacgcga gttggaccag gggggtgaaa gcgcgcgcgc gggcttcgag
     7441 gcccgcgtga aagctctcgt cgaggatgtt ccggaattgc cgaacttcag ccgcttccac
     7501 aatgcgttgc gcgagaacag cgagagcgcg acgtccgaag gcgacatgcg cgcggctttc
     7561 tacctcgctt acaaccctga agcatgcgag tacatccgtc tgaatgacga acgcattgac
     7621 gatcgtctga agaacggtcc ggaactggtc tcggccaaat tcgtgattcc ctatccaccg
     7681 ggattcccca tcatggtccc cggacaagtc attacgccgg aaaccattgc gttcatgcgc
     7741 aagctggatg tgaaggaaat tcacggctat aacgccgcgc ggggactgga actgctcaag
     7801 ccggaagccc tggacgcact ccgccagagg ccctgaccat ggtcgtgcct gtacggcaat
     7861 gcgtggtgaa cccggagagt agtgaggggc ttgccactta agtgctggct gacagcaggc
     7921 ttttatagtc tgctgctcat cgtgatgggc acagcggtat tgccaggcaa gttgcgattt
     7981 atcgactacg gcgtcgtcct tgcgacttcg gccatcgcgg ccgaagcacc gattcaacgg
     8041 cgggctcggc ctgcagtatg aggacgaacc ccgtcgcctg tcgaggcggt gattaaagtg
     8101 cttatgaact ggctgacaaa gtcactgcag gcttcgccag aactggcgat gttcctcgcc
     8161 gtgggcatcg gctactgggt cggcggcatc aggttcggca agttctcgct gggaacggtc
     8221 accgcgtcgc tgatcgtcgc gttggggatc gggcagctgc atgtggaagt cagccgtgaa
     8281 cttcgctggg gactcttcct gctatttctg ttcgccaatg gctatgctgc gggtccgcag
     8341 ttctttcagg cactcaagga ggatggcgtc aagccgatgc tcctgtcggt cacagtgatg
     8401 gtggtggggc ttggcacggc atgggcgatg gcgcgcctgt tcaagctcga cccaggaacg
     8461 gccgccggga ttttttgcgg tggactgacg cagtcagcag caattggcac tgccaccgat
     8521 gccatcatga acctgccgtt gccgctggag gagcgcaagc tgttggcaaa tcacgttgcc
     8581 gtcgccgatg cgctgacgta cctgtttggt gcgatcggca tgatcctgtt tgtcagctcg
     8641 ctggctccgc gtcttctgcg cgtcgacctg cgcgaggagg cggctgcgct ggagagaaaa
     8701 ttcggcattc ggcgcgacac ggcgggcgtt tttgcagcac accaaagatt tgccgtgcgt
     8761 tcgtatcgtc tttccagcag cgggtttgca ggcaagcggg tcgacgaggc tgagcgtagc
     8821 gaagctccga tgcgttattt catcgagcac atcctgcgcg acggcagcgc catcgaggtt
     8881 cggccagaaa ccgtgctgca ggcaaacgac acggtcaccc tctacggaca aatgccagct
     8941 ctggtgaaga tcggtccgag gttcggcacg gaattgcacg accccgaatt gctccagttt
     9001 ccaatcgaaa tactgaaagc gacggtgacg aacaaagcac tgtgtggggt tccgctcgaa
     9061 gcactgatgc gggcgccaga gttcgacttc cgcagcatcg ggctgcgctc gattacgcgg
     9121 gggacacaac agatcccaat cggtaccgaa actgtcctga accgcggcga cgtgctcgaa
     9181 ctggtcggcc cgcagcgcgc ggtcgaacgg gttactgcgg agatcggcta tgcactgcgt
     9241 cccaaccatg cctccagtct gtccattctg gggctcggca tactgtgcgg cgcgctggtt
     9301 gggctgcctt atgtcatgct cggcaacatc aggcttacgc ttagtgtgag cgtcggcaca
     9361 ctggtcgccg gcctgctgct cgggtggttg cgctcactgc ggccgacgct tggcgacatt
     9421 ccccagccag cactcgatct gatgatcaac ttcgggctgg cagcctttgt tgcgtgttcg
     9481 ggcctccagg cgggtcccga gttcgttcgc gcggtgaagg agctgggact cggcatcctg
     9541 gtggccggtg cggtggtgac tttgctgcca caggcagtgg cgcttctggt ggggcgctat
     9601 gtattgcgga tgaatccttt gatgttgctg ggcgcgctgg ccggcgcaca gacatttacg
     9661 gccgccctgg ctgcggtgca ggacaaagcc ggcagccgta ttccggtact gggctacacc
     9721 gtgccctatg caacgtcgaa tatcctgctg accaccggtg gatcgatcat ggttgccttg
     9781 atggcgtagc ccggccaccg tggttgcgca gacatggacc gaccgcgtcg gccaggatct
     9841 caacgccgga ttcatcagcg acaagtctgc ctggcggcac tcattgccaa gcccacggtt
     9901 gcatgtggcg catcgtgatt cgcgagagcc gaagcggcat cgctttcgcc gacgaagcgc
     9961 ccgcccgaag gttcgaaacg aacctctggt caacgatcgc aaggcagtgt gcaacgggcg
    10021 tcaaccggga cagcgggatt tgagctggat gatctttcat acggggaccc catgagcgtc
    10081 atatcttatg cagcgagaat ccaaccatgc ccacgccttc gcccgtccgt gcacctgcgc
    10141 cgtttcgcat gctggatcgc gcgccatctg gcccgcgtgg ctggctttag tcagctttct
    10201 ggttctgtct ttactgggcc ttcatggagc ggttgctgcg ccactggccg caggcctgca
    10261 aaaccttgtc gctaatgcgc cggcctcgac ggcgcaagct gcgtcgtcac cttcggcggc
    10321 cagtcaggcc gaccaggcac gcgcgctcga taccctgatt gactcgctag agaacgatgc
    10381 acaacgaaaa gcgctggtct cccatttgaa gggactacgc gctgcctcac gcagcgccgc
    10441 acccgaggcg ccggcggcgg ctgctcaacc taatagcgat ctgctcggcg ccattgcgtc
    10501 gggcattgcg tccgtcgaga cggacctgag cgagggccgg acacccttgc attattgggc
    10561 agcccgttcc tccgcggcgc taggcgatct gcaggccatt ggggccggca agaacgggga
    10621 gtcgttcggc gaggtcctgc tttggatgac cgcaacgctc gcgtgctggg gcgcgtgcgc
    10681 ctgcatattg atctatctgc agaggcgcat tgttgcaaga tacggctgga aggtcgcgct
    10741 cgaaccgaat ccatcatcgg ttgaattgtt gatcttcgct gtctggcgaa cggcgccctg
    10801 gatcgtcgca tttgtggcgg tcgtggtgtt cgtgcagcga gtgctcgaca cattgggcag
    10861 cacgctgggg ctcgtgatcg cttacgcgat cgtcgcgggc tcgatcttct cggcgatctg
    10921 catgatcacc ctttcgatct tcaacacggc acatcggcgt gtcgcattac gtcagttgct
    10981 cacgcaaagc cgctggccgc ttttcgtgat cggagcatcc ggcgcactcg gcgacgcgac
    11041 ggccaacccg gacgtcatag gcctgcttgg cgcgaacctc gccggagtcg tgtcggcatc
    11101 cgctaatgtg acggcagccg ttctgatcgg atatttcgcc cttgctttcc ggcggcctgt
    11161 cacgcatctg atacgcaacc gtccgtatgc tcaaaggcgc gatcacaagc tcgccacgga
    11221 agcgctcgat atcctcgcat cgctttggca cattccaatc ctggtgatcg cgatcgcgtc
    11281 ggtggttgcg attatcgatg cgcgcggttc ggaagagagc gttttgcagc cgtcgcttct
    11341 gagtgcgctg ttgcttgtcc tcacgctttt catgtcggcg ctgctgatgc acgtgacgcg
    11401 gcgaagggac acacgcatac accgccgcgc gccgcatctg atgagactct tgcgtttcgc
    11461 cagtaccgtg gttgttttcc tgacgtggct cgccttcatt cgattcgaac tggacctgtg
    11521 gagcggtccg atcgcgcgaa cgatcaagca cagtgcgatg acacctggct tcagacatgc
    11581 gctggttgta gtcatcgcga cggtattcgg cgcctggttc atatggatca gttgcgacac
    11641 cataatcctg aaagctatag gaccgagcag ttcgcgtaac aaggcacttg atcccggcgt
    11701 gcgcgcacgc acgatgttgc ctctcgtgcg taacgctgtg ttcgcaacgg ttgcaagtct
    11761 ggccgccgtc gtcgccgcgg ctagcctggg catcaatttg acgcctctgc ttgccggcgc
    11821 cggcgtgata ggtcttgcag tgggttttgg tgcgaaggca ctcgtaaccg atctgatcac
    11881 ggggctcttt atcattgttg aggaaacgat ctcggtgggg gactggatcg aggttggcag
    11941 cgaccatgcc ggtaccgtca tggatctgac aatccgaacg gtgcggttac gcgatggaca
    12001 aggtgccgtt cacaccatcc cgttctcaca gatcaagatc gtgaaaaacc tgtcgcgcga
    12061 ctttgccaac gcggtattcg aggtgcgcgt gccgttccac gccgatatgg aagacgtcac
    12121 gcggatgatt ctcgaagtga gcgccgattt gttcgcagac tttaaccttc gcaaggaaat
    12181 acttgggccc gtcgaggtgt gggggcttga ccgtttcgaa gccaactggc tagttgtgaa
    12241 agcgcagatc aagacgcggc cattacagca gtggagcgtg gcgcgcgcct tcaatgcggg
    12301 gctcaagcgg aagatggacg aggcgggcat tgaaattccg gtgccgcaga tgctgctcaa
    12361 tacatcggga ggtcggggac cgtcgacaca gtctgacgac gctacccgca acgagagccg
    12421 ataagcgaac gacgctgatc ccgaactgac aggcactggg cgatggcgct ggtcgcattg
    12481 ctgtccgggc cgcggaaact caccgacgta gcggctccgc tgaacgactg gcgtaccatg
    12541 gcggccgcgc cggatggctt ccggcgggct tcaatatttg gtagcgggct cagacgcacc
    12601 aagaacgaca ccacgggctt ttgcagcagc gagtgcgacc ttcagacgct tgctgctcgc
    12661 tggcccgacc gtcagaggta cgagactgat ggacttatcg atgcttggat ataacgatta
    12721 tccgatgcaa taaagagcca aaacctttca tc
//
LOCUS       DATASH010000082         2923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4559503,
            whole genome shotgun sequence.
ACCESSION   DATASH010000082 DATASH010000000
VERSION     DATASH010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4559503"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VHV99_02440"
     CDS             complement(<1..477)
                     /locus_tag="VHV99_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX3634347.1"
                     /translation="MKIHEYQGKEILRKFGVAVPRGKPVFSVDDAVKAAEELGGPVWV
                     VKAQIHAGGRGKGGGVKVAKSLEQVREYSNQILGMQLVTHQTGPEGQKVNRLLIEEGA
                     DIKKELYVGLVIDRVSQKIVVMASSEGGMDVEEVAEKTPELIHKIAVDPATGLKDAE"
     gene            complement(474..1214)
                     /locus_tag="VHV99_02445"
     CDS             complement(474..1214)
                     /locus_tag="VHV99_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HEX3634348.1"
                     /translation="MPLSPPVSRQLRHRRAIRAEAYERADGLWDVEACLTDEKPRDVV
                     LASGVRPNGQPIHELWLRITIDRKLNVVDAEASSDWVPYPGLCQASNPAYRALIGLNL
                     RQNFRRESARLLGGTVGCTHLTELCAMLPTAAIQAFAGDVWNTDKGAPGASAGSGDES
                     SNSTGEHSTDNPPFQLGRCHALRFDGEAVQQFYPRWYGRAPYTADRAASSGDGAARQT
                     GKGGNPSGMNDGSGNEVQSNSQTEGNHA"
     gene            complement(1416..2033)
                     /gene="recX"
                     /locus_tag="VHV99_02450"
                     /pseudo
     CDS             complement(1416..2033)
                     /gene="recX"
                     /locus_tag="VHV99_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049957.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
     gene            complement(2491..>2923)
                     /gene="recA"
                     /locus_tag="VHV99_02455"
     CDS             complement(2491..>2923)
                     /gene="recA"
                     /locus_tag="VHV99_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920416.1"
                     /note="catalyzes the hydrolysis of ATP in the presence of
                     single-stranded DNA, the ATP-dependent uptake of
                     single-stranded DNA by duplex DNA, and the ATP-dependent
                     hybridization of homologous single-stranded DNAs; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEX3634349.1"
                     /translation="PETTTGGNALKFYASVRLDIRRIGSIKKNDEVIGNETRVKVVKN
                     KVSPPFREAIFDILYGEGISRQGEVIDLGVQAKIVDKAGAWYSYNGERIGQGKDNARE
                     FLRENPDIAREIENRIRESLGVNAMAEAATSVSAEVAGEEE"
BASE COUNT          545 a          892 c          889 g          597 t
ORIGIN      
        1 ttcagcgtcc ttcagacccg ttgccggatc gacggcgatc ttgtggatca gctcaggcgt
       61 cttttccgcg acttcttcga cgtccatgcc gccttcgctc gatgccatca cgacgatctt
      121 ctgcgaaacg cgatcgatca ccaggccgac atacagttcc ttcttgatgt cagcgccttc
      181 ttcgatcagc agacgattca ccttctggcc ttccggaccg gtctggtgcg tgacgagctg
      241 catgccgagg atctggttcg agtattcacg aacctgttcc agcgacttgg cgaccttcac
      301 gccgccgccc ttgccacggc cacccgcgtg gatctgagcc ttcacgaccc ataccggacc
      361 gccgagctct tccgcggcct tgaccgcatc atccaccgag aagaccggct tgccgcgcgg
      421 taccgcgacg ccgaatttcc gcaggatttc cttaccctgg tactcgtgaa tcttcatgcg
      481 tgattccctt cagtctgaga gttggattga acttcgtttc cgctaccgtc gttcatgccg
      541 gacgggttgc cgcccttacc tgtctggcgg gctgccccgt cgcctgacga tgccgcgcga
      601 tccgctgtat acggagcgcg gccataccag cgcggataaa actgctgcac cgcctcgccg
      661 tcgaaacgca gcgcgtggca gcgtcccagc tggaatggcg ggttatcggt ggaatgctcg
      721 ccggtgctgt tagacgattc gtctcctgaa ccagcgctcg cgcccggcgc gcctttatcc
      781 gtattccaca cgtcgccggc gaacgcctgt atcgcggcgg tgggcagcat cgcgcacaac
      841 tcggtgagat gcgtgcaacc caccgtgccg cccagcaaac gggcagattc acgacggaag
      901 ttttgacgga gattgagccc gatgagggca cggtaggcgg gattgctggc ttggcacaac
      961 cctggatagg gcacccagtc ggacgacgct tcggcgtcga cgacgttgag cttgcgatcg
     1021 atggtgatgc gaagccagag ttcatggatc ggctgaccat tgggccggac gcccgacgca
     1081 agcaccacat cgcgcggttt ttcgtcggtc aagcatgctt ccacatccca caggccatcg
     1141 gctcgctcat aggcttccgc tctgattgcg cgacggtggc gcaactgacg ggatacgggc
     1201 ggagagagcg gcatgcggga aggaaaggcc ggattggaaa acccatgaat tttagcatac
     1261 tgggtatttg agacagatcg gaatgcgcat ggccgcccgg caggccggag agcacggcag
     1321 acgctatctc aaagtgaggg tgtgacggca aagcgaggag gttttcgcac tggagcctat
     1381 acggtaccca tacgcggtac cggctggtcg ccgcttcact cctcgtcaat acctttgaga
     1441 attttgccaa tcgtggcgcc cgagaaaccg cgcgatgcca ggaagcgcgc ctgcttggcc
     1501 cgctcggcag ccgtttcggg aagccggccg aatttcttct gccagacggc ctgcgcacgt
     1561 gcgagttcgg tttcacgtag ctgcgcactg gcttcttcga ctagcgtttg gtcgaccgcg
     1621 tgctgcttca actcgccgac gatccggctc gcacccaagc gcgacgagcg ccgatggacc
     1681 aggctttcgg caaaacgtga atcggacagc cagttttcag cttccagcat atcgagcaag
     1741 gtgtcgagcg aatcggtttc ttcgacgaat ggcttcagct tgcgcgccag ttcggagcgg
     1801 ctgtattcac gccgggacag atagccgagc gcccgtcctt ttagcgaacg agccgggcgt
     1861 tgggattttt ttgcatcgtc ctgaccgggc ttcgcctcgc caggtgtgcg gcgcgagcgg
     1921 gtataggtgg tttcggacgg gtctgttgtg tcttgcgcgg aagcgtcaga ctgcggctga
     1981 cgacctgcgg cgcgatcgtg cgcgtcgaag ggttcaaacg gctcgaacgg atcggcgtcg
     2041 ccggctgcga caaagggatc gggagctgac gcggatgagc gctgggacgc ggcgttgctg
     2101 cccggatagc gcggcgcacg cgaggtggaa cgcccgaaag agcttccaga agatttgccg
     2161 aaggaactat tagacgaact gccagacgag ccgtcgaacg aactgcccga agaacgaccg
     2221 gaggaactgc cgaacgagct gttggaggaa cgtccgaatg agctgcccga ggaacgacca
     2281 gacgaactgc cgaacgaatt tcccgacgac cgcccaaacg accggttgga ccgagaagtc
     2341 gacgaggagg atgttgttga tgaggaaggt tccgacgaag aggatgacga cgactcgaac
     2401 gaatcgtcat caccgtcacg cgggccgtcc gcgttgcgtc cagcgttggc agcgttagac
     2461 agcggccggc ccttgcgtat cacgtgcggg ttactcttct tcgcccgcga cttcggcgct
     2521 gacgctcgtc gccgcttcag ccatggcatt cacgcctagc gattcacgga tacggttttc
     2581 gatctcacgg gcgatgtccg gattttcacg caaaaattcg cgcgcgttgt ccttaccctg
     2641 accaatccgt tcgccgttgt agctatacca ggcgccggcc ttgtccacga tcttcgcctg
     2701 cacgccgagg tcgatgactt cgccctgacg cgaaatgcct tcgccgtaaa ggatgtcgaa
     2761 aatcgcttcc cggaacggtg gcgatacttt gttcttcaca accttcacgc gcgtttcgtt
     2821 gccgatcact tcgtcgttct tcttgatcga gccgatccgg cgaatgtcca gacgcaccga
     2881 tgcgtagaac ttcagcgcgt taccacccgt ggtggtttcc gga
//
LOCUS       DATASH010000083        17461 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1593019,
            whole genome shotgun sequence.
ACCESSION   DATASH010000083 DATASH010000000
VERSION     DATASH010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 17461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17461
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1593019"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            315..1775
                     /locus_tag="VHV99_02460"
     CDS             315..1775
                     /locus_tag="VHV99_02460"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX3634350.1"
                     /translation="MPTLVLKDPTLLKTKSYIAGEWQPADDDTTFEIKNPATGETIAT
                     VPRMGTIETRRAIDTANAAWPAWRATTAKQRATILRKWHDLMLENADDLAKILTTEQG
                     KPLAEAKGEILYAASFLEWFAEEGKRVNGDTIPTPASDKRIVVTKEPIGVCAAITPWN
                     FPAAMITRKVGPALAAGCPIIVKPAEATPLSALALAVLAERAGVPRGVFNVLTGEPKV
                     IGAELTGNPIVRKLSFTGSTPVGRLLMAQCAPTVKKVSLELGGNAPFIVFDDADLDAA
                     VAGAIASKYRNSGQTCVCTNRFYVHDKVYDAFADKLRVAVEQLKVGRGTDEGVTQGPL
                     INEAAVLKVESHIEDALAKGARIVTGGKRHALGHGFFEPTVLADVTPVMKVARDETFG
                     PLAPLFRFSSDEEVIRLANDTEFGLASYFYSRDIGRVWRVAEALEYGMVGINTGLISN
                     EVAPFGGVKQSGLGREGSHYGIDDYVVIKYMCVGGI"
     gene            complement(1823..2959)
                     /locus_tag="VHV99_02465"
     CDS             complement(1823..2959)
                     /locus_tag="VHV99_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012406135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX3634351.1"
                     /translation="MKYTLALGSMLLGISASSFAQSSVTLYGIIDTGIELVTHANAAG
                     DSVVRMPGITGEFPSRWGISGKEGLGGRLSAVFVLESGFNTKGGTLNQGGRLFGRQAY
                     VGLESPYGTLTFGRQYTMTYWAILDSDLLGPDIYGGTGSFDQYMPSARSDNTIVYKGT
                     FSGFTFGATYSFGRDSAGTGNSPGQGTCVGSIPGSSQSCREWSTMLRYDMNGYGVAGA
                     YDEQRGGPGAAANLYDGTAPFALTQSGDKDVRMQLNGYGHIGPVKLGAGWLGRHVETV
                     SPALPDVRSNLYYVTAAYNLTPAFILDGGVYRIINRADDTRGTIAALRTTYLLSKRSA
                     VYLQGAYLFNSPHAAYTVSQGGGGTTPAPGIGQLGVMAGIRHSF"
     gene            complement(3171..4589)
                     /locus_tag="VHV99_02470"
     CDS             complement(3171..4589)
                     /locus_tag="VHV99_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018434294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3634352.1"
                     /translation="MTSYVPPAGSASASADEVGAPLAGNRAGVEEERHAVDIGRTLDE
                     GPYTTMQKIVVFLAALSIVLDGFDSQLIGFAIPVLIKEWGVTRAAFAPVVAAGLIGMG
                     IGSACAGLLADRFGRRWAVIGSVLVFGAATCGIAFASDIVTIALLRFIAGLGIGGALP
                     SSTTMTAEFTPARRRTLAVTATIVCVPLGGMVAGLFAHEILPTYGWRGLFLIGGVLPL
                     ALAALLFFTLPESPRFLARQPRRWPELRRLLGRMSRPVASGSVFTDVREQAIEKHTGF
                     TALFREGLALDTLAIWCAFCLCLLAVYSAFSWLPTMLSTQGLSVSVAGSGLTAYNLGG
                     VVGALLCAVVIARTGSRWPLIICSAGGAASALLLLGVNISDHTGLLIFGLGVHGLFVN
                     AVQSTMFALCAYVYPTRVRATGTASALAFGRLGAIASAFAGAVVITAGGASSYLWMLG
                     VAMLIVMVALMVVKRHIPKPGV"
     gene            complement(4818..6269)
                     /locus_tag="VHV99_02475"
     CDS             complement(4818..6269)
                     /locus_tag="VHV99_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015601129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEX3634353.1"
                     /translation="MQSISMLINGTAVQASNGATFERRNPLDGEVATRAPAATVADAV
                     AAADAAAAAFPAWSAMGPSERRALLMKAAHALEARAEAFAAVMAAETGASAMWAGFNV
                     HLAAGGLMEAAALTTQIGGELIPSDVPGSLAMGVRQPAGVVLGIAPWNAPVILAVRAI
                     ALPLACGNTVVLKGSEICPGTHGLIIEVLQEAGLPKGVVNFITNAPADAGTVVEALIA
                     HPKVRRVNFTGSTHVGKTIAATCARYLKPSVLELGGKAPLIVLDDADLDAAVNAAAFG
                     AFANSGQICMSTERIIVDERIADTFVARLAQKARGLPLGDPRKGPVVLGSVVDMSTVE
                     RCNALIDDALAKGATLVCGGKADSTLMPATLLDHVTPAMRIYADESFGPVKGIVRVNG
                     EDEAVACANDNEYGLSSAVFSRDVARAMNVARRIESGICHVNGPTVHDEAQMPFGGVK
                     GSGFGRFGGQAGIAEFTDLRWITVQTTPRHYPF"
     gene            complement(6325..7944)
                     /gene="mdlC"
                     /locus_tag="VHV99_02480"
     CDS             complement(6325..7944)
                     /gene="mdlC"
                     /locus_tag="VHV99_02480"
                     /EC_number="4.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018423345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoylformate decarboxylase"
                     /protein_id="HEX3634354.1"
                     /translation="MTSNAPRSDASGLSQHASITVRDAVIDFMRRVGMTSVFANPGST
                     ELPLFRDFPQDFRYVLGLQEAVVVGMADGFAQATRNASFVNLHSAAGVGNAMGNIFTA
                     FRNRTPIVITAGQQARSILPFDPFLASTQATELPKPYVKWSIEPARAEDVPLAIARAY
                     HVAMQEPRGPVLVSIPVDDWDRAAEPVPARVVSTEMRPEPFVLEQIGAALDACERPAF
                     VVGGAVDRAGGWDAVVSLAERHNARVFVAPMTGRCAFPEDHRLFAGFLPAMREKIVEL
                     LGGHDLIFVIGAPAFTYHVEGHGPHLPAGATLCQLIDDPAIAAWAPAGTAAIGNIRLG
                     VADLLTRPAPKARPLPAPRLACPRAEPSALMSVAFALQTLADLRHADDIVVEEAPSSR
                     PVMQNTLPFTRSGTFYTMDSGGLGYGMPAAVGVALAKPGTRVIGVIGDGSSMYSIQAI
                     YSAVQMKLPITFVILNNSRYAALQDFAPEFGFGPADPVQGTDLPGLDFVALAAGMGCP
                     GTRVTDAERLHDVLRDALASSGPMLVEVVVA"
     gene            8201..9109
                     /locus_tag="VHV99_02485"
     CDS             8201..9109
                     /locus_tag="VHV99_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006755172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3634355.1"
                     /translation="MTFNLQQLHAFATIVASGSLGRAAEVLHVTQPALSRTIKRLEDQ
                     VGAPLFERHSKGMQLTAIGSALLPHATLLQREADYAREEIDAMRGLAKGTIRVGAVAS
                     IATLVLPLAVSGVLARWPNLRVEIMEGVWDRLADSLMKHEIDLALSMAVPDTDEIAAI
                     ADCRWEDTSHVVAALDHPLRGKVDLCLGDTLDQQWCVPPRGTGPFEHLQHVFAEHGLG
                     MPPIAVETRSITVLKSLVTRAGFLSWMATPMYDTESAAGVFDTLPISGLDARRTLTAF
                     RRRQGILPSPAVKLLEQLRQLTAVQE"
     gene            complement(9258..9584)
                     /locus_tag="VHV99_02490"
     CDS             complement(9258..9584)
                     /locus_tag="VHV99_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4148 domain-containing protein"
                     /protein_id="HEX3634356.1"
                     /translation="MKSLIEAAVIAALITAPLAAFAQSNQPVTRAEVRAQLVQLEKAG
                     YDPALSNADDYPANIQAAEARVAAQNAVAQTSGYGSATNGSSQAGSRTETTLSSYSVP
                     IVNVGH"
     gene            9740..9896
                     /locus_tag="VHV99_02495"
                     /pseudo
     CDS             9740..9896
                     /locus_tag="VHV99_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590665.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine dehydrogenase"
     gene            10055..10144
                     /locus_tag="VHV99_02500"
                     /pseudo
     CDS             10055..10144
                     /locus_tag="VHV99_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007749058.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
     gene            complement(10248..11090)
                     /locus_tag="VHV99_02505"
     CDS             complement(10248..11090)
                     /locus_tag="VHV99_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HEX3634357.1"
                     /translation="MKMNRAFWLILTLLFANPVWAFQSRVVTIPSAAMHKSFDATVVL
                     PDQYTHGKGADRFPVVYLLHGSGGDYTDWTANSHIGKLADRYHVILVMPDGGHESWYI
                     DSPVDPSSRYETYVGSEVVGYIDTHFRTIATRQARAITGLSMGGFGALHIALDRPDEF
                     GAAGSISGAVDPRGCEDEPGIDQVFGDPTRHADFWNNEAIVENARGFGNAHIALTIDC
                     GVSDSLVQSNRTLHARLVELGVPHDYAERPGGHTWKYWSNAVQYQVLFFASAFRRSGG
                     AAQT"
     gene            complement(11309..12085)
                     /locus_tag="VHV99_02510"
     CDS             complement(11309..12085)
                     /locus_tag="VHV99_02510"
                     /EC_number="1.-.-.-"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX3634358.1"
                     /translation="MNVLLVYAHPEPRSLNGSLKDFSVKRLEDAGHVVQVSDLYAMKW
                     KASLDADDSLNRQGDARFNPSLDSKRAFEAGMQSQDIAREQEKLKWADAVILQFPLWW
                     FSMPAILKGWVERVYAYGFAYGVGEHSDARWGDRYGEGSMAGKRAMLIVTTGGWESHY
                     SPRGINGPIDDVLFPIQHGVLYYPGFDVLPPFVIHRTSRIDEARFSTIRHALGQRLDD
                     LWKTAPIPFRRQNAGAYDVPALTLRPDVSPDQVGFSAHIE"
     gene            12157..13071
                     /locus_tag="VHV99_02515"
     CDS             12157..13071
                     /locus_tag="VHV99_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3634359.1"
                     /translation="MSGIAMNNLRRLDLNLLVTLDVLLSEHNVTRAAERLNFSQPSVS
                     VHLAKLRDIFGDPLLLPGPRGMRPTARAEELREPLRLALDALEHAVSPASPFNPAEAT
                     HTWRVAATDYAESTILLPVLAGLRATAPGTRLAIVEAVPPRIAKQAEQGDIDLAFHTR
                     EGAPPGLRQRLLFKERYVLVGRANHPRLKRRPTLAQFCKLEHVIVSPDGGGFSGVTDD
                     ALAESGLTRRVVLSVPHFLFMMSVLANTDLVGMLPSRLVRDNSALKIVEAPVEVPGYE
                     MAMLWHERSHRDPAHQWLRDCIVDSVAS"
     gene            complement(13094..13975)
                     /locus_tag="VHV99_02520"
     CDS             complement(13094..13975)
                     /locus_tag="VHV99_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HEX3634360.1"
                     /translation="MSNFTAASTFPLAGHPVQRMGYGAMQLAGPGVFGPPKDRDAALA
                     VLREAVASGVNHIDTSDFYGPHVTNQLIRQALHPYPDDLVIVTKVGAVRGDDGAWLPA
                     LEPQDIERGVHDNLRNLGVDALDVVNMRIMGNVHAPAEGSIEKQVAALAELQQRGLVR
                     HIGLSNVTPAQVAEAQGIAEIVCVQNHYNVVHREDDALVDELAARGIAYVPFFPLGGF
                     TPIQSTALSTLAQTIGATPMQVALAWLLHRAPNILLIPGTSSLGHLRENLQAAQLSLS
                     DEVRAELDAIGKRDAAA"
     gene            14082..14975
                     /locus_tag="VHV99_02525"
     CDS             14082..14975
                     /locus_tag="VHV99_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3634361.1"
                     /translation="MEFNDLAAFVSVARAGGFRDAARISGVSASSLSIAVRRLESKLG
                     MRLLNRTTRSVAPTEAGLRLIEKLTPLFSEMEAALDVLNGFRDKPTGTLKLNVPSSAA
                     RIVLATIIPQFLKAYPDIRVEVVVEDGFVDVLSIGCDAGIRYDERLEQDMIAIPIGPR
                     VQRFATAAAPDYLDTHGRPEHPRDLLTHACLRGQFSGGAMPTWYFERDGEVIQLNPSG
                     PLLVRPGAAIDLAVSTAVAGVGVIHLFEDMLRPHLESGALEPILVPWWQRFSGPFLYY
                     PGRRHLPAPLRVFVDFLKAAG"
     gene            complement(15117..16280)
                     /locus_tag="VHV99_02530"
     CDS             complement(15117..16280)
                     /locus_tag="VHV99_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX3634362.1"
                     /translation="MKKSLFVVALSGAFAISAHAQSSVTLYGLIDTGLIYTNNQSGHS
                     NWQEVSSSTQNTVFGLKGSEDLGGGLHAVFKLEQGFLLNNGAQAFSGDGFGSQAWVGL
                     QSDSYGTVTFGRQFDVMNDLVGPLTAESNTWGGNMAAHPFENDNLAANSVVVNNTVKY
                     ASPTYRGVTFETMYGFSNQAGAFANNRSYGFAVSYAQGPVNLAAGYLQFNNAGGAAGG
                     AVTQGDTSANFLAQRQRIWSLGGNYTFGPATFGLVWSHTQMDNASGVYSFGTGTYLGA
                     NDASAGTLSGSLRMDNFEANAKYAITPALSVSGAYTYTHGAYNGSSPGWNTAMLQTDY
                     SLSKRTDFYLEGVYQNVHGAPADSVLSHAMINTLSPSSSNTQVAVTVGMRHAF"
     gene            complement(17108..>17461)
                     /locus_tag="VHV99_02535"
     CDS             complement(17108..>17461)
                     /locus_tag="VHV99_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007744502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634363.1"
                     /translation="ATGTHGNTVTTSGTVVTTGVVTTGGTAVVVAPKPVVVVPPPVVY
                     VPPKPVVVVAPPVVYAPPKPVVVVPPPVVYVPPPPPRAVYVAPRMVYVTPAPRPVVWV
                     PAHWVGNVWVPAHWS"
BASE COUNT         3495 a         5551 c         5230 g         3185 t
ORIGIN      
        1 caagctcgtt cagacgcctc accagatcaa cgatcagtgc gtccttctgc tccggtgtca
       61 gatccttgat gtcgggcatc ttcgtcatat ccctcactat gcacagcctg gtagcagttt
      121 acaagcgctg aatagttacg cagcaggcaa agaaaagtag gtgcctgccc cgcacagggg
      181 cgacgctaat agaccaataa gaaatcaagg aaaggccaaa aagcaagttc agggaaaggc
      241 caacaccgca ggcatacaga cacaagcgca gcacagcaaa aaccccaaat tcaaaaccca
      301 ggagcaaaac ccaaatgccg acgctagtcc taaaagaccc cacccttcta aaaacaaaaa
      361 gctacatcgc aggcgaatgg caacccgccg acgacgacac aaccttcgaa ataaaaaacc
      421 ccgccacagg cgaaacaatt gccacggtgc cccgcatggg cacaatagaa acccgccgtg
      481 caatcgacac ggccaacgcc gcctggccag catggcgagc caccaccgcc aaacaacgcg
      541 ccacgatcct gcgcaaatgg catgacctca tgctggaaaa cgccgacgat ctggcaaaga
      601 tcctgaccac cgaacaaggc aaacccctcg ccgaagccaa aggcgaaatc ctctacgccg
      661 cctcgttcct ggaatggttc gccgaagaag gcaaacgcgt caacggcgac acgatcccca
      721 caccagccag cgacaaacgc atcgtcgtca cgaaagaacc gatcggtgtc tgcgccgcca
      781 tcacgccgtg gaatttcccc gcggccatga tcacgcgtaa agtcggcccc gccctcgccg
      841 ccggctgccc gatcatcgtc aaaccggccg aagccacgcc actgtcggca ctcgccctag
      901 ccgtgctggc cgaacgcgca ggtgtgccgc gcggcgtgtt caacgtcctc accggcgagc
      961 cgaaagtcat cggcgcggaa ttgaccggca atccgatcgt gcgcaaattg tccttcacgg
     1021 gctcgacgcc ggtgggccgt ctgctgatgg cgcagtgcgc gccgacggtc aaaaaagtgt
     1081 cgctcgaact cggcggcaat gcgcccttca tcgtgttcga cgatgcggat ctggacgcgg
     1141 ccgtcgcagg cgccatcgca tcgaagtacc gcaatagcgg ccagacctgc gtttgtacca
     1201 atcgcttcta cgtgcacgat aaggtctacg acgcattcgc cgacaaactg cgcgtcgccg
     1261 tcgaacaact caaggtcggc cgcggaaccg acgagggcgt cacgcaaggc ccgctgatca
     1321 acgaagccgc tgtgctcaag gtcgaatcgc atatcgaaga cgccctcgcc aaaggcgctc
     1381 gcatcgtcac cggcggcaag cgccacgcgc tcggtcacgg cttcttcgag ccgactgtgc
     1441 tcgcggatgt cacgccggtc atgaaggtcg cgcgcgacga aaccttcggg ccgctcgcgc
     1501 cgctgttccg cttctcgtcg gatgaagaag tgatccgcct cgccaacgat accgaattcg
     1561 gcctcgcgtc gtacttctat agccgcgata tcggacgcgt ctggcgcgtc gccgaagcac
     1621 tcgaatatgg catggtcggc atcaacaccg ggctgatctc caacgaagtc gcaccgttcg
     1681 gcggcgtcaa gcaatccggc ctcggccgcg aaggctcgca ttacggtatc gacgattacg
     1741 tcgtcatcaa gtacatgtgc gtgggcggga tctaaagaaa taacggccac atcggcttat
     1801 ctgacgtggc tgttttgact gctcagaacg aatggcgaat gccggccatc acgccgagtt
     1861 gtccgatgcc cggcgcgggc gtcgtgcctc cgccgccctg gctcaccgta taagcggcat
     1921 gcggactgtt gaacagatac gcgccttgca ggtaaaccgc ggaacgcttc gagagcaggt
     1981 acgtcgtgcg taacgcggcg atggtgccgc gcgtatcgtc ggcgcgattg atgatgcgat
     2041 acacgccgcc gtccagaata aatgcaggcg tgaggttata cgccgccgtg acgtagtaga
     2101 gattcgagcg cacgtccggc aatgccgggg ataccgtttc gacgtgacgt ccaagccaac
     2161 ccgccccgag cttgaccggc ccgatgtgtc catagccgtt caactgcatc cgcacgtcct
     2221 tgtcaccgga ctgtgtcagc gcaaagggcg cggtgccgtc gtacagattc gcggccgcgc
     2281 ccgggccacc gcgttgttcg tcgtaggcgc cggccacacc ataaccgttc atgtcatagc
     2341 gcaacatcgt cgaccattcg cggcaactct gcgaactgcc cggaatcgac cccacacagg
     2401 tgccctgccc cggcgaattg ccggtacccg ctgaatcgcg gccgaaggaa tacgtggcgc
     2461 cgaacgtgaa gcccgagaac gtgcctttat acacgatcgt gttgtcgctg cgcgcgctcg
     2521 gcatgtactg atcgaacgac cccgtgccgc cgtaaatgtc aggcccgagc agatcggaat
     2581 cgaggatcgc ccaatacgtc atcgtgtact ggcgcccgaa cgtcaacgtg ccgtatggac
     2641 tctcgagacc tacgtacgct tgccgcccaa acagccggcc accctgatta agtgtgccac
     2701 ccttggtgtt aaagccgctc tccagtacaa agaccgcgga caaccggccg cccaaccctt
     2761 ccttaccgct aatcccccaa cgcgacggaa actcgccggt gatgcccggc atgcgcacga
     2821 cgctgtcgcc cgccgcgttt gcatgcgtca ccagttcaat gccggtatcg atgatcccgt
     2881 acagcgtcac gctgctctgc gcgaacgacg atgcgctgat gccaagcaac atactgccca
     2941 aagcgagtgt gtatttcatt tgtctccagg tctccgtggt aggtgctttg catgagcgcg
     3001 caacgatcca gacgccactc atacgaagcg tttttatgta gcgatactaa ttacagacgt
     3061 gacagcaccg gccgctattc aagccacccg atacgtgaaa gactgactgc ttcagatagc
     3121 ggcaatccgc gcatcgcgcc cgatcgccgc gcttgcggac gttgcgcgtc tcagacgccg
     3181 ggcttcggaa tatggcgttt gaccaccatc agcgcgacca tcacgatgag catcgcgacg
     3241 ccgagcatcc acaagtacga cgatgcgccg ccggccgtaa tcaccacggc cccggcaaac
     3301 gcgctggcga tcgcgccgag ccggccgaat gcgagcgccg atgcggtgcc ggttgcacgc
     3361 acacgggtcg gatagacata agcacacagc gcaaacatcg tcgactggac agcgttgacg
     3421 aacaggccat gcacgccgag gccgaagatc agcaagccgg tgtgatcgga aatattcacg
     3481 ccgagcagca gcaatgcgct cgccgcaccg cccgcgctgc agatgatcaa cggccaacgc
     3541 gagccggtgc gagcaatcac cacggcgcac agcaacgcac ccaccacgcc gccgaggttg
     3601 tacgcggtca gtcccgagcc cgccaccgac accgacaagc cctgtgtgct cagcattgtc
     3661 ggcagccagc tgaaagcgct atagacagcc agcaggcaca ggcagaacgc gcaccagatc
     3721 gccagcgtat cgagcgccag gccctcgcgg aacagcgcgg taaagccggt gtgcttttca
     3781 atggcctgct cgcgcacgtc ggtgaatacg ctgccgcttg ccaccggacg cgacatccga
     3841 ccaagcaggc gtctgagttc cggccagcgc cgcggctgcc gggcgagaaa acgcggcgac
     3901 tcgggcaacg tgaagaacag cagcgcagca agtgccaacg gcagcacgcc gccgatcaga
     3961 aacaagccgc gccatccata cgtcggcagg atttcatgcg cgaacagacc cgccaccatg
     4021 ccgccgagcg gcacgcagac gatcgtcgcg gtgactgcca aagtgcggcg gcgagccggt
     4081 gtgaattccg cggtcatggt cgtcgagctc ggcaacgcgc cgccgatgcc gaggccggcg
     4141 atgaaacgca gcagggcaat cgtcacaatg tcggatgcga aggcgatgcc gcaggtggcg
     4201 gcgccgaaca ccagcacgct gccgatcacg gcccagcgac ggccgaagcg atcggccagc
     4261 agtccggcgc atgcgctgcc gatgcccatg ccgatcaatc cggccgcgac caccggcgca
     4321 aaagccgcgc gcgtgacgcc ccactccttg atcagcacgg gaattgcaaa accgatcagt
     4381 tggctatcga agccatccag gacgatcgat aacgccgcga gaaacaccac gatcttctgc
     4441 atggtggtgt aaggaccttc gtcgagcgtg cggccgatgt cgacggcatg gcgctcttcc
     4501 tcgacacctg cccgattgcc ggcgagcggc gcgccgacct catcggcgga cgcactggcc
     4561 gatccggccg gcggtacata actggtcatg aagctgtctc ctggattgtg tgcgcgattc
     4621 ttcccgccgc gcttttgttg tcacggtttt taatcagtat gtgagcgagc gttcggcctt
     4681 tgacattgcc gtttcgcgca aaagccatga cctgtatgca tggcttgcac gacgctcgta
     4741 tcgctgtggt gatctggcgc gccgcgcatc acagcgatat gcggcgatac acagcggcca
     4801 atgcggcgct cagcgcttca gaacggataa tgacgtggcg tggtttgcac ggtgatccag
     4861 cgcaagtccg taaattcagc gattcccgcc tgcccaccga aacgtccaaa gccgctgccc
     4921 ttcacgccgc caaacggcat ttgcgcttca tcgtgaacag tcgggccgtt gacgtggcag
     4981 atgcccgatt cgatacgcct cgcgacattc atcgcgcgcg ccacatcacg gctgaacacc
     5041 gcggatgaaa gcccatattc gttgtcgttc gcacaggcca ccgcttcgtc ttcgccgttg
     5101 acgcgcacga tacccttcac aggcccgaac gattcatcgg catagatgcg catggcgggc
     5161 gtgacgtgat cgagcagcgt ggcgggcatc aaggtgctgt ccgctttgcc gccacaaacg
     5221 agcgtggcgc ctttggccaa cgcgtcgtcg atcagcgcgt tgcagcgttc caccgtgctc
     5281 atatcgacga ccgatcccaa caccaccggt cccttgcgcg ggtcgccgag cggcaggcca
     5341 cgcgctttct gcgcgagcct ggcaacgaac gtatctgcga tccgctcgtc gacgatgatc
     5401 cgttccgtgg acatgcaaat ctgccccgag ttggcgaacg cgccgaatgc agcggcgttg
     5461 acggcggcat ccagatccgc gtcgtcgagc acgatcagcg gcgcttttcc gccgagctcc
     5521 agcaccgacg gtttcagata gcgcgcacag gtggccgcga tggtcttgcc gacatgcgtg
     5581 gaaccggtga aattgacgcg ccggactttc ggatgcgcga tcagcgcctc gaccaccgtg
     5641 cctgcatcgg ccggtgcgtt ggtgatgaag ttcacgactc ccttcggtaa gcccgcttcc
     5701 tgcaagactt cgataatcag gccgtgcgtg ccggggcaga tttccgaacc cttcagcacg
     5761 acagtgtttc cacaggcgag cggcaatgca atggcacgca ccgcaaggat caccggcgcg
     5821 ttccagggcg cgatgccgag cacgacgccg gccggctgac gcacgcccat cgcgagactg
     5881 ccgggcacgt cggacggaat cagctcgccg ccgatctgcg tggtgagcgc ggccgcctcc
     5941 atcaaaccgc cggcggcgag gtgcacgttg aagcccgccc acatcgccga tgcgcccgtc
     6001 tctgccgcca tcaccgcggc gaacgcttcg gcgcgcgctt ccaacgcatg cgctgccttc
     6061 atcagcagcg cgcggcgttc gctcggcccc atcgccgacc atgccggaaa cgctgctgcc
     6121 gcggcatcgg cggcggctac cgcgtcggcg accgtcgctg cgggcgcacg cgtcgcgact
     6181 tcgccatcga gcggattgcg gcgctcgaag gtggcgccgt tgcttgcctg caccgccgtg
     6241 ccgttgatca acatcgagat gctttgcatc gtgtttgtct cctgcggaaa gtttgatgaa
     6301 tgcgggcgag aactgagctc gcgctcacgc cacgacgact tcgaccagca tcggtccgga
     6361 cgacgccaac gcatcgcgca gaacgtcatg caagcgttcg gcgtcggtca cgcgcgtacc
     6421 cggacaaccc atgcccgcgg caagcgccac aaaatcgagt cccggcagat cggtgccttg
     6481 cacagggtca gccggaccaa aaccgaattc cggcgcaaag tcctgcagcg cggcatagcg
     6541 tgaattgttc aggatcacga acgtgatcgg cagtttcatc tgcacggcgc tatagatggc
     6601 ctgaatcgaa tacatgcttg agccgtcgcc gatcacgccg atcacgcgcg tgccgggctt
     6661 cgccaacgcc acgccgacgg cagccggcat gccatagccg agaccgccgc tgtccatcgt
     6721 gtagaaggtg ccgctgcgcg tgaacggcag ggtgttctgc atcaccggcc gggaactcgg
     6781 cgcctcctcg accacgatat cgtccgcatg gcgcaggtcg gcaagggtct gcaacgcgaa
     6841 cgccaccgac atcagtgccg acggttccgc gcgcggacat gcaaggcgcg gtgcgggcaa
     6901 aggacgtgcc ttcggcgcgg gccgggtcag cagatcggcc acgccgaggc gaatattacc
     6961 gatcgccgcg gtgccggccg gcgcccatgc cgcaatcgcc ggatcgtcga tcaactgaca
     7021 tagcgttgca cctgcaggca aatgcgggcc gtgaccttcc acgtgatagg tgaaggccgg
     7081 cgcgccgatc acaaagatca ggtcatggcc gccgagcaac tcgacgatct tctcgcgcat
     7141 ggccggcaag aagccggcga acaggcgatg gtcttcgggg aacgcgcaac ggcctgtcat
     7201 cggcgcgacg aacacgcggg cgttgtgtcg ttcggcgaga ctgacgactg cgtcccagcc
     7261 gccggcccgg tcgacagcgc cacccaccac gaaggccggc cgttcgcagg cgtcgagcgc
     7321 cgcgccgatc tgctccagca cgaacggctc cggccgcatt tcagtactga ctacgcgggc
     7381 cggcaccggc tccgcagcgc ggtcccaatc gtcgacggga atcgacacca gcaccggccc
     7441 gcgcggctcc tgcatcgcca cgtgataagc gcgcgcaatg gccagcggca catcttcggc
     7501 acgcgccggt tcgatgctcc atttgacgta cggcttcggc aattcggtgg cttgtgtcga
     7561 cgcaaggaac gggtcgaacg gcagaatcga gcgggcctgc tgcccggcgg taatcacgat
     7621 tggtgtgcga ttgcggaacg cggtgaagat attgcccatt gcattgccca cacccgccgc
     7681 cgaatgcaga ttgacgaacg aagcattgcg ggtggcctgc gcaaaaccat cggccatgcc
     7741 gaccacgacc gcttcctgca agcccagcac atagcggaaa tcctgcggaa aatcgcggaa
     7801 caacggcaat tccgtcgacc ccggattggc gaataccgaa gtcataccga cgcggcgcat
     7861 gaaatcgatc acggcgtcgc gcaccgtgat cgatgcgtgt tgagagagtc ccgatgcgtc
     7921 tgaacggggt gcgttactgg tcatgcctgt ctcctgtcga ccagagttgg cgctttagca
     7981 cttactctgg tcccatgcaa agctgtcgat ccgagctggc gctttagctg tcgaccggaa
     8041 ttcgcgcttc agcgcgttac tccggtccca tgcaaagcac ttactctggt cccatgcaaa
     8101 atagttgcgg tcaatgagcg ccgtttcttg atggctcatt atcaaaaact cgaggttgat
     8161 tgaaaattgc ctttttcgag aaaaggcatt acttttgcgc atgactttca atcttcagca
     8221 attgcatgcc ttcgcaacca tcgtcgcgag cggcagcctt ggccgtgccg cggaagtgct
     8281 gcacgtcacg cagcctgcgc tgagccgcac catcaagcga ctcgaggatc aggtcggcgc
     8341 gccgctgttc gaacggcatt cgaagggtat gcagcttact gcgatcggca gcgcgctgct
     8401 gccgcacgcc accctgctgc aacgcgaagc cgattacgca cgcgaggaaa tcgacgcgat
     8461 gcgcggcctc gccaagggga cgattcgcgt cggcgcggtg gcgagcatcg cgacgctcgt
     8521 gctgccgctc gcggtgagcg gcgtgctcgc gcgctggccg aatctgcgcg tggagatcat
     8581 ggagggggtg tgggaccggc tggcggacag tctgatgaaa cacgagatcg atctcgcgct
     8641 cagcatggcc gtgccggaca cggacgaaat tgccgcgata gccgattgca gatgggaaga
     8701 cacgagtcat gtggtcgccg cgctcgatca tccgctgcga ggcaaagtgg atttatgtct
     8761 tggcgacacg ctcgatcagc agtggtgtgt gccgccgcgt ggcacgggtc cgttcgagca
     8821 tctgcaacac gtgtttgccg agcatgggct cggcatgccg ccgattgcgg tcgagacgcg
     8881 ctcgatcacc gtgctgaaaa gcctggtgac gcgtgcggga tttctaagct ggatggctac
     8941 gccgatgtac gacaccgaaa gcgcggcagg cgtattcgac acgttaccga tttcgggcct
     9001 cgacgcacgc cgcacgttga cggcgtttcg ccgccgtcag gggattctgc cgagtccggc
     9061 ggtgaagttg ctggagcaac tcaggcagct gactgcggta caggaataac tggctgcacg
     9121 cagctaaacg ggcaggctgc ctccttcagc ctcctcaatg cacagcagcc ttgctttcac
     9181 atcgacgtga gagcaaggcc gcttgtgcaa cgacacgaat caagctggcg cgagacgact
     9241 gctcgcgccg cagccgatta atgacccacg ttgacgatcg gaaccgagta cgagctcagc
     9301 gtggtttccg tgcggctgcc tgcttgcgac gagccgttcg tggctgaacc ataaccgctg
     9361 gtttgagcga cggcgttttg tgcggcgacg cgcgcctcgg cagcctgaat gtttgcgggg
     9421 taatcgtcgg cgttcgacaa cgccgggtcg tagccggctt tttccagctg caccagttga
     9481 gcgcgcacct ccgcgcgggt caccggttgg ttggattggg caaacgcagc gagcggtgca
     9541 gtgatgaggg cggcgataac agcagcttcg atcagcgatt tcatgacgac tacctccaga
     9601 gattattttt gccgtgctgc aaacctgttt gtttgcagcc agtgaacgta gtctaagagt
     9661 cggcaggcct agggtaaaca cctaataagc caattcactg ttgctataat cagcacaatc
     9721 tcaataggac atagctgaaa gttcgaaaat cgatgaccga gtagtcgacg ggacgtctga
     9781 acaggtgggg acgcgcccga tcaaacagct tgaaacggta tcgtgacgat tgcataacga
     9841 gggtgctggc tgcgattgga gaggccaagg cgcggcacgg cttgcttcca gcggaacgat
     9901 gctgtgtact tgcacacgcc cgcccgcgtt tgaatgcgcg gatgcgccaa cccgagcggc
     9961 gcgccgggac tcatcgcgat cagctttcag cggcaggccg gcgcacgatc gccgcagcgt
    10021 gcgctgttca tgggaataaa ggacacgatt agctatgcct gcgccgcagc ctgcttcgtt
    10081 gccctgctat cgcgacgtcg tgcgcgctga gtgggtcggc tacaacggtc atttgcgcga
    10141 cgcgtgatcg gcttgcttgc gaagccacca aacgcacgcc ctgctgtgtg tcgaccggac
    10201 cgctggacgc agcgcgcctg gacggtctgc cgcttgcgaa gcgcgcctca cgtctgcgcc
    10261 gcgcccccac tccgcctgaa cgcactggca aagaagagca cctgatactg caccgcgttg
    10321 gaccaatact tccacgtatg gccgcccggc cgctccgcgt aatcgtgcgg cacgccgagt
    10381 tcaaccagcc gcgcatgcag cgtccggttc gattgcacca gcgagtcgct caccccgcaa
    10441 tcgatcgtca gcgcaatatg cgcatttcca aaaccacgcg cgttctcgac aatcgcctcg
    10501 ttgttccaga agtctgcgtg acgcgtcgga tcgccaaaca cctgatcgat ccccggctca
    10561 tcctcgcagc cgcgcggatc gaccgccccg ctaatacttc ccgcggcgcc gaattcatcc
    10621 ggccggtcca gcgcgatgtg caacgcccca aacccgccca tgctcaaccc ggtaatcgcg
    10681 cgtgcctgcc gcgttgcgat cgtgcggaaa tgcgtatcga tatagccgac gacctcgcta
    10741 cccacatagg tttcgtaacg gctgctcgga tccacaggac tgtcgatata ccagctctcg
    10801 tgaccgccgt ccggcatgac gaggatcacg tgatagcgat cggccagttt gccgatgtga
    10861 gaattcgccg tccagtccgt atagtcgccg cctgaaccgt gcaggagata gacgacgggg
    10921 aagcgatcgg cacctttgcc gtgcgtgtat tggtcgggca gcaccaccgt tgcatcgaac
    10981 gacttatgca tggcggcgct gggaatggtg acgaccctcg actgaaacgc ccagacgggg
    11041 ttcgcgaaaa gcagcgtcag aatgagccag aaagcacgat tcattttcat gtttgtcgct
    11101 ctcccctttc tacgtgtcct aagaacactg tccggcatct ggcggcaaaa ctctgacgac
    11161 gactctagca atggcgactt acaaccaaat ttcagtgaag catacggcct tgtcagcttg
    11221 gcgccgctcc aacggggcgg gacggcgcaa actaatcgtc gattcaacag ctacgtccgg
    11281 cacgtgaatg cactggacaa tattgcgtct attcaatgtg cgccgaaaag ccgacctgat
    11341 ccggggagac atcggggcgc aaggtgagcg ccgggacatc gtacgcaccg gcgttctgtc
    11401 ggcgaaacgg gatcggcgcg gtcttccaca gatcatcgag gcgttgaccg agcgcgtgac
    11461 ggatcgttga gaatcgcgct tcgtcgatcc ggctggtccg atgaatcacg aacggcggca
    11521 gaacgtcgaa accggggtaa tacagaaccc catgctgaat cgggaacagc acatcgtcga
    11581 tcggcccgtt gatgccgcgc gggctgtagt gagattccca gccgccggtc gtaacgatca
    11641 gcatcgcccg cttgcctgcc atggagcctt cgccatagcg gtcgccccag cgcgcgtcgg
    11701 agtgctcgcc gactccatac gcgaagccgt atgcatagac gcgttccacc cagcccttca
    11761 ggatcgcggg catcgagaac caccacagcg gaaactgcag aatgacggcg tccgcccact
    11821 tcagtttctc ctgctcgcgt gcgatgtcct ggctctgcat gcctgcctcg aaagcacgct
    11881 tcgagtcgag agagggattg aaacgcgcat cgccttgccg attcaggctg tcgtccgcat
    11941 ccagtgatgc tttccacttc atcgcataca gatccgatac ctgcacgacg tgtccggcat
    12001 cctctaatcg tttgaccgag aaatccttca gcgacccatt caatgaccgg ggttccgggt
    12061 gggcatagac gagaagaaca ttcatgacct gagctccttt gccagaaagg aaatacgata
    12121 agttctggtc aggtatattg gaaatgaatt gccaatatgt caggtatagc catgaataat
    12181 ctcagaagac tggacctcaa cctgctggtg acactcgatg tcctgctgtc ggagcacaac
    12241 gtgacccgtg ccgcggagcg cttgaacttc tcccaacctt ccgtgagcgt gcatcttgcc
    12301 aaactgcggg atatcttcgg tgacccgttg ttgttgccgg ggccacgcgg catgcgcccc
    12361 accgcgaggg ccgaagaatt gcgcgaacct ttgcgcctcg cgctcgatgc gcttgagcat
    12421 gcggtatcgc cggccagccc attcaatcca gccgaagcga ctcatacgtg gcgcgtggcc
    12481 gcgaccgact acgccgaatc gacgatcctg ctgcccgtgc tcgccggctt gcgcgcgacg
    12541 gcgcccggca cccgccttgc gatcgtggag gccgttccgc cacgtatcgc aaagcaggcg
    12601 gaacagggtg acatcgatct ggctttccat acccgcgaag gcgcgccgcc cggattgcgc
    12661 cagcgattgc tcttcaagga gcgctatgtg ctggttggcc gcgccaatca cccgcggttg
    12721 aagcggcggc ccacgctagc gcagttctgc aaactggagc acgtgatcgt ctcgccggat
    12781 ggaggtgggt tcagcggtgt cacggatgac gcgctggctg agagcggtct cacgcgcagg
    12841 gtggtgcttt cggtcccgca cttcctgttc atgatgtcag tcctcgcgaa cacggacctc
    12901 gtggggatgc tgccgtcgcg tctggtgcgc gacaacagcg cgcttaagat cgtcgaggcg
    12961 ccggttgaag tgccgggtta tgaaatggcg atgctttggc atgagcgctc gcatcgcgac
    13021 ccggcgcatc aatggctgcg ggactgcatc gtggactcgg tggccagctg aacacgcgcg
    13081 tgactatgca cgatcacgca gccgcatccc gtttgccgat ggcatccagt tcagcgcgca
    13141 cttcatcgct caggctcaat tgggctgcct gcaggttttc acgcagatgc ccaagcgagg
    13201 aagtgccggg gatcagcaga atgttcggcg cgcgatgcag aagccacgcg agcgccacct
    13261 gcatcggcgt ggcaccgatc gtttgggcaa gggtggacag cgctgtcgat tgaatcggcg
    13321 tgaatccgcc cagcgggaag aacggaacgt aagcgatccc cctggccgcc agttcgtcga
    13381 ccaatgcatc gtcttcccgg tgcaccacgt tgtaatgatt ctgcacgcag acgatctccg
    13441 cgatgccttg cgcctcggcg acctgggccg gggtcacgtt gctcaaaccg atgtgacgta
    13501 caagcccgcg ctgctgaagt tcggccagcg ccgcgacctg cttttcaatc gatccttcgg
    13561 cgggtgcgtg cacgttgccc atgattctca tattgacgac gtcgagcgcg tcgacaccga
    13621 gattgcggag gttgtcgtgg acgccgcgct cgatatcttg cggctcgagc gccggcagcc
    13681 atgcgccgtc gtcgccacgc accgcaccga ccttggtgac gatcacgaga tcatccggat
    13741 aaggatgcag cgcttgccga atcaactggt tggtgacgtg gggtccgtag aagtcgcttg
    13801 tatcgatgtg attgacgccc gacgccaccg cttcgcgcag caccgcgagc gctgcatcgc
    13861 gatctttcgg cggcccgaat acgccgggcc cggccagttg catggcgccg tagcccatgc
    13921 gttgcactgg atggccagcc agcggaaagg tgcttgcagc ggtgaagttc gacatggcgc
    13981 gctccttgat gtgaggggtt gagcgtcagt atgaagggca ttgcgttgtt cgataatccg
    14041 gcctaaattg cacgggttgt taaacatttt gaacaacggc catggaattc aacgatcttg
    14101 ccgcatttgt ttcggtcgct cgcgccggcg gctttcgcga cgccgcgcgc atcagcggcg
    14161 tgtccgcctc gagcttgagc attgcggtac ggcggctcga gtcgaaactc ggtatgcgcc
    14221 tgctcaaccg aaccacgcgc agcgtggccc cgaccgaagc tgggctgcgc ctgattgaaa
    14281 agctgacgcc tctgttcagc gagatggaag ccgcgctgga cgtcctcaac ggcttccggg
    14341 acaaaccaac cggcacgctc aagctcaatg ttccgtcgag cgccgcacgc atcgtattgg
    14401 cgaccatcat tccgcagttc ctgaaggcct atcccgacat tcgtgtggag gtggtggtgg
    14461 aggacggctt tgtcgatgtg ctgtcgatcg gttgcgacgc gggcattcgt tatgacgagc
    14521 ggctcgagca ggacatgatc gcgattccga tcggtccgcg tgtgcagcgt ttcgctacgg
    14581 ccgcggcgcc ggactatctc gacacgcatg gcagacccga gcatccgcgt gacctgctca
    14641 ctcatgcgtg cctgcgtggt caattctccg gcggcgccat gccgacctgg tatttcgagc
    14701 gcgacggcga ggtgatccag ttgaatccgt caggcccgct gctcgtcaga ccgggcgcgg
    14761 caatcgatct tgccgtcagt accgcggttg caggtgtggg ggtgattcac cttttcgagg
    14821 acatgctgcg gccccacctc gagagcggcg cgttggagcc gatcctcgtg ccgtggtggc
    14881 aacgcttttc ggggccgttt ctttactatc cgggccgtcg tcacttgccg gcaccgttgc
    14941 gcgtatttgt cgatttcctg aaggcggctg gttagcgagc cctctaacga tgccttgtac
    15001 cgattcgact gctgagaagc cgtggagacc atcgtacccg cgaaacccga ggtgtcgacg
    15061 tatgcctggc agagtgcagg catacgtcga gtgcggctca catgacgcga gaccgatcag
    15121 aatgcgtggc gcatgcccac ggtaaccgcc acttgcgtgt tcgacgacga cggcgagagc
    15181 gtattgatca tcgcatgcga gagcacggag tccgcaggcg caccgtgcac gttctgatag
    15241 acgccttcaa gatagaagtc ggtgcgcttg ctgagcgaat agtcggtctg cagcatcgcc
    15301 gtattccagc ccggcgacga accgttgtag gcgccatgcg tgtaggtgta cgcgcccgat
    15361 acgctcaatg caggcgtgat tgcatacttc gcgttcgctt cgaagttgtc catgcgcagc
    15421 gacccactca gggttccggc ggaagcgtcg ttcgcaccga gatacgtgcc ggttccgaag
    15481 gagtagacac ccgacgcgtt gtccatctgc gtgtggctcc agaccaggcc gaatgtagcc
    15541 ggtccgaacg tgtagttacc gccaagcgac cagatgcgct ggcgttgcgc aaggaagttc
    15601 gcgctcgtgt cgccctgggt aacggcaccg cccgccgcac cgcctgcgtt attgaattgc
    15661 aggtagccgg cggcaagatt gaccggccct tgcgcatagg agacggcgaa accatatgaa
    15721 cggttgttcg cgaaggcgcc cgcctggttg ctgaacccat acatcgtttc gaacgtgacg
    15781 ccgcggtacg tcgggctcgc gtacttgacc gtgttgttga caacgaccga attggcagca
    15841 aggttgtcgt tttcgaacgg atgcgcggcc atgttgccgc cccacgtgtt ggactccgcc
    15901 gtgagcgggc cgaccaggtc gttcatcacg tcaaattggc ggccgaacgt cacggtgccg
    15961 taggaatcgc tttgcaagcc gacccacgcc tgagagccga aaccatcgcc ggagaaagcc
    16021 tgggcgccgt tattcagcag gaatccctgc tccagcttga agaccgcgtg caagccacca
    16081 ccgagatcct ccgaaccctt gaggccgaac accgtgtttt gcgtcgagct gctcacttcc
    16141 tgccagttgc tatggccgga ctgattgttc gtgtagatca atcccgtatc gataagacca
    16201 tagagcgtca cgctgctttg cgcatgcgcc gaaatagcaa aagcgccgga aagcgcgacc
    16261 acgaataaag actttttcat attgtttggc tccatgaatg ggcggtgatg gcgtaatcag
    16321 ttgtgtgcat tcgataccca ttggcaagcg cgtcgaccgc ccgcaaaacg tggcaacgga
    16381 aaggcaacgc attcgcggag cagtgccacg cgaattgcta aaacgtgcaa tagagtttgt
    16441 tgattattgc gtatagcgcc ttgcaatgaa ttgcgaatgg cgcatgctga ctacagtggt
    16501 ttttttcaat tgggttacac aggcgcgtct tgcaagcaat accgaaaacg aacaacgcga
    16561 tgatatggct tggaagataa ataacacgtc aaaaataaaa atgacgttgc gtcaaaagaa
    16621 cactcgtgac ctgcgttagc gagttgtcag cgccacgcct gcgtagactc gcccaccacg
    16681 cgcgacagat gcgcctgcat cgcctctcgc gccgcggccg gatcgcgaaa atacgctgat
    16741 gatcttcctg ccagattccc ggcatgacgg acatcgtgtc cgccatttac gacgagccga
    16801 cggagacttc catgccacgc aatcgatggg ccgctgtccc tgcctctcgt tgcgtggttg
    16861 atccgcgacc tccgccactg ccgacaccgc caccgctgcc gagagcaccg gcaccgtctc
    16921 gtggctcgat ctgtttcgct atcgcacggt gtcgggcatg atgattgggc tgttctgcct
    16981 caacttcgcg atttactttt tcatcacgtg gttcccgagt tatgcctgcg cacttccctg
    17041 ctcgctcgtc gacgacggac cgatttgttg ccaggcctcg tcgcccgcgc tcactcgcgc
    17101 gtttcgctca tgaccagtgc gccggcaccc agacgttccc cacccaatgc gccggcaccc
    17161 acacgacagg gcgcggcgcc ggcgtcacat agaccatgcg cggtgcgaca tacacagcac
    17221 gcgggggcgg cggcggaacg tagacgaccg gtggcggtac gaccacgacc ggcttcggcg
    17281 gcgcgtacac aaccggcggc gctacgacca cgaccggctt cggcggcacg tacacaaccg
    17341 gcggcggcac gaccacgacg ggcttcggcg caaccacgac cgccgtgcca cccgtcgtga
    17401 cgacgccagt ggtcacgacc gttccgcttg tcgtcaccgt gttgccgtgc gtacccgtgg
    17461 c
//
LOCUS       DATASH010000084         7872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4757944,
            whole genome shotgun sequence.
ACCESSION   DATASH010000084 DATASH010000000
VERSION     DATASH010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7872
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4757944"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VHV99_02540"
     CDS             <1..231
                     /locus_tag="VHV99_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HEX3634364.1"
                     /translation="LLNTIAARFSVQVSEQIAAKSIPAIGAVLGAMVNTVFIDHFQQV
                     AHGHFTVRRLERQYGQETVEAAYQAIDVTLDG"
     gene            complement(201..1166)
                     /locus_tag="VHV99_02545"
     CDS             complement(201..1166)
                     /locus_tag="VHV99_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012399779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3634365.1"
                     /translation="MSWQSALACWYLRRQFRPETLKPGVNVEKARALTAKRAWSPRVP
                     AGWRLREAYGPHDWPLHGEWIQRADGVSVAGNGPTVLYCHGGGYYFCSPRTHRSLVYG
                     LAMRANASIFSLDYRLAPEHRFPAALDDVTAAYRQLVADSTPPESIVIAGDSAGGGLA
                     LATLVALRDAGDPLPAGCLLFSPWTDLAAAGATIRSNDGLDPMFSGPAIARAAKVYLG
                     DTSATHPYASPVYADLRGLPPLFIMAGSTEVLLDDSQRVADKAHAAGVDCELEVWKKM
                     PHVWPLFSPFIPESNRALDRAATFVRRVTSGTTRAAQPSSVTSIA"
     gene            complement(1283..1717)
                     /locus_tag="VHV99_02550"
     CDS             complement(1283..1717)
                     /locus_tag="VHV99_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEX3634366.1"
                     /translation="MIAFRKLNFAVVVWVLASGVAFADTVALKADLEPSSEVPPRVSH
                     GHGMLNATFDTSTKSLQWTITYEGLTGPATAAHFHGPAPVGQNAKIQVPIGKDVLASP
                     IKGSAALTEQQVTDLMAGQWYFNVHTAQNPMGEIRGQVLPAN"
     gene            complement(1912..3039)
                     /locus_tag="VHV99_02555"
     CDS             complement(1912..3039)
                     /locus_tag="VHV99_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX3634367.1"
                     /translation="MKKILILAAVSATFACAANAQSSVTLYGVVDAGFTYVNNEVTAS
                     DASKHGASYGLTSGNLSGSRWGMRGREDLGGGYAAVFTLENGFSLANGTTAQSGRSFG
                     RQAFAGISSDYGTVTLGRQYDAVVDYLGPLTATGTWGGTYFAHRGDNDNANASMRINN
                     SVKFQSANYDGFSFSGLYAFSNQAGAFANDRAYSAGMGYSNGGLKLAAGYLQAQGLGG
                     NANGAIQDGAPNLGVAGERQRQRTWGAGGSYVFGPATVGLVYTQSRFNFAYNDASVRY
                     SNYEVNAHYNLTPAFSLGAAYTYTQALQNTPGVQNGSSHWNQFGLQADYALSRRTDLY
                     AEGVAHIGANGNTVGAQVFGTDAPSSNKNQVVVTTGIRHRF"
     gene            3567..4484
                     /locus_tag="VHV99_02560"
     CDS             3567..4484
                     /locus_tag="VHV99_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3634368.1"
                     /translation="MRVFLKVADNGSFGRAAASLDLSNAVVTRYVALLETHLDTRLVN
                     RTTRSLSLTEAGLAYAEGCRQLLDQLEAIESSISQSANDPSGTLKLVAVASFSLFGLT
                     PLLQRYRLQCPRVKLSVTLLHRPVDLIEEGFDVGIVVPGQVSGGTLIKRALFKVRSVA
                     VASPAYLAARGTPCAPVQLAAHPFLAPSANVHSAAWCFVDASETEVTVNMEAAYAVNN
                     AVMLRQAALADMGITILPENQVSADLLSGALVQVLPGYRIKGADKEVSLVYPGRRHVS
                     AKTRSFVEFTVDYFRNRAGSTLSSIFPNS"
     gene            complement(4715..>7872)
                     /locus_tag="VHV99_02565"
     CDS             complement(4715..>7872)
                     /locus_tag="VHV99_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3634369.1"
                     /translation="VAPSLDDALKMVENGQADVLVGNVAAVDILLKQRYTGVLKILGT
                     VGGSDSLGFAVRPDLAPLAGLIDRALLAMPPAEKQRIRQKWVTGSAGDLGTWSVTAVR
                     LLPLLIGIGLVLLVTLRAYVLLQREVRLRKQTERELALQLNFQETMMEMVPYPLVAKD
                     LDGRYIAINRAYEEACGLLRESVIGRTAMDIQTWGETNSRVLDELTRKMLIDGEPAQA
                     ELQFERGTGDLRHGLFWTRICHGSDGKPSCVLGTMVDITNIRRAEMLARETERRLFDV
                     TRSLPAVVFQLRRTADGAYSFPYIGGDTRHLLGGDGASSMSREEIDFRRVCEQDRPFV
                     LAELEQSARCETPVNMEFRFDVERELKWARAELVPRREEDGSVVWSGYWVDASVEHAR
                     SEELARARDVAEAASRAKDEFVAMMSHEIRTPMNGVLGLVEVLERTPLNADQGEMLSM
                     IHESAGALLQILDDLLDYSKIEAGRLTIEAEPIDMRELVDNAVGLLAGRAHEKGLKVR
                     VDIAPDVAATLRGDSVRLRQILFNLLGNAIKFTPEGEVDVQVAVVGQTGDAQTIEMTV
                     KDTGIGIAPDVQARLFEPFVQAESSTTRRFGGTGLGLTICRKLIDLMSGPLELHSEPG
                     VGTCMTVCLTMPVESQRYSMSGLRGKRGVVATGDARVAQALMHFGQALGLELRCMLPG
                     ATELGDPVALAGADMIFVSEDVMLPAGVGSGSRILCLTEKPKPTGYRILDNNVRVSVN
                     PISWRGLGAACAAAMTGLPSVAPRSAGMPRMTEGANVPPDRERAIASGRLILVAEDHP
                     VNQELIRHQLALLGFACDVTNDGVEALAALEQTSYGCLITDCHMPNLSGYELARRIRE
                     QEQGDTHHLPILGITANTAPEDLKLCRDAGMDDCLVKPTRLATLRDYLSRWFGTDSAW
                     QAAPVETVNAPPASGPDARGVPEPFVPVDLSHMTQLWGSESTVKALLDSFVSSVREDV
                     LALSPLLERVEVERLREWHHRVAGAASVLQYPPLLNALEDYRRDITLKTPERLRCDGL
                     SLARKCNAMLDGIEEQAALLA"
BASE COUNT         1480 a         2563 c         2312 g         1517 t
ORIGIN      
        1 ctgctcaaca cgattgccgc gcgcttttcg gtgcaggtga gcgagcagat cgccgccaag
       61 tcgattccgg cgatcggcgc ggtgctcggc gcgatggtca atacggtgtt catcgaccac
      121 ttccagcagg ttgcgcacgg ccacttcacc gtgcgtcggc tggaacggca atacggccag
      181 gaaacggtcg aagccgccta tcaggcaatt gatgtgacgc tggacggctg agcggccctc
      241 gtcgtgccgc tcgttacacg ccgcacgaaa gtcgcggcgc gatcgagcgc gcggttcgac
      301 tcgggaatga acggagagaa cagcggccag acgtgcggca tcttcttcca cacttcgagt
      361 tcgcagtcga cgcctgccgc gtgcgccttg tcggcgacac gttgcgagtc atcgagcaat
      421 acctcggtgc tgcccgccat gatgaagagc ggcggcaaac cgcgcaggtc tgcatagacc
      481 ggcgaagcgt acggatgcgt ggcggacgtg tcgcccagat agacctttgc cgcgcgcgcg
      541 atggcagggc cgctgaacat gggatcgaga ccgtcgttgc tgcggatggt ggcgccggct
      601 gcggccaggt cggtccacgg cgagaacagc agacagcctg ccggcaaagg atcgccggca
      661 tcgcgcagcg cgaccagcgt ggcgagcgca aggccgccgc ccgctgaatc gcccgcaatg
      721 acgatcgact cgggcggcgt actgtctgcg accagttggc ggtaggcggc ggtcacatcg
      781 tcgagcgcgg ccggaaaacg atgctcaggg gcgagacgat agtcgagtga aaaaatcgag
      841 gcgttggcgc gcatcgccag gccatatacg agcgagcgat gcgtacgcgg cgaacagaag
      901 tagtagccgc cgccgtggca atagagcacg gtcggaccgt tcccggcaac cgatacgcca
      961 tcagcgcgct ggatccactc gccgtgcagc ggccaatcgt gtggcccgta cgcttcacgc
     1021 agacgccaac cggccggcac gcgcggcgac caggcgcgtt tggccgtcaa cgcacgcgct
     1081 ttctccacgt tgacgcctgg tttcaaggtt tccggtcgaa actgcctacg cagataccag
     1141 catgcgagtg cactttgcca gctcatcggg acacgctcct tcgatagtgt cccgtcatgg
     1201 tacgtgcgtt cgccgcagca ccggtggacg acggcttcta atcctgaaaa agcgcgcgcc
     1261 accggtaagg ctttctttgt gcctagttag ccggcaagac ctgcccgcga atctcgccca
     1321 tcggattctg tgcggtatgc acgttgaagt accactgccc cgccatgaga tccgtcacct
     1381 gctgttcggt gagcgccgcc gatcctttga tcgggctcgc gagcacgtcc ttcccaatcg
     1441 gcacctggat cttggcgttc tgcccgaccg gcgcgggacc gtggaaatgc gcggcggttg
     1501 ccgggccggt cagcccttca taggtgatgg tccattgcag ggacttggtc gacgtatcga
     1561 acgttgcgtt caacatgccg tgcccgtgac tcacgcgcgg cggaacttcg ctcgacggct
     1621 cgagatcggc cttcaacgcc accgtgtcag cgaaggcgac gcccgacgcc aacacccaca
     1681 ccaccaccgc aaaattcagt tttcgaaacg caatcatggt cttctccgga cttgatgcat
     1741 cgccgacccg acattcggac acgcgcgaag caaccacata ctagtgcaaa tccgtcgagc
     1801 ctgtttgcgc cagcggcgat gcgacaaggt cgccttcgcc gcgtgcacgc taataaaaaa
     1861 agcaccgcga gcggtgcttt tccttccacc attgatgctt cagaagccgc tttagaagcg
     1921 gtgacggata cccgtcgtca cgaccacctg gttcttgttc gacgacggtg cgtcggtgcc
     1981 gaacacttgc gcgccgaccg tgttgccgtt cgcgccgata tgcgccacgc cttcggcata
     2041 cagatcggtg cggcgcgaca acgcgtagtc cgcctgcaga ccgaactggt tccagtggct
     2101 cgaaccgttc tgcacacccg gcgtgttctg caacgcctgc gtgtaggtgt acgccgcgcc
     2161 gagcgagaac gccggcgtca ggttgtaatg cgcattgact tcgtagttgc tgtaccgcac
     2221 ggaggcgtcg ttgtatgcaa agttaaagcg gctttgcgtg tacaccagac cgaccgttgc
     2281 cggaccgaac acgtagctgc cgcccgcgcc ccacgtacgc tgccgctgac gttcacccgc
     2341 cacaccgagg ttcggtgcgc cgtcctggat cgcgccattc gcattgccgc cgagaccttg
     2401 cgcctggagg taaccggccg ccagcttcag gccaccgttc gaatagccca tgcctgcgct
     2461 gtaagcgcga tcgttggcga atgcgcctgc ctggttcgag aaggcgtaca agccgctgaa
     2521 cgagaagccg tcataattcg cgctctggaa cttcaccgag ttgttgatgc gcatcgacgc
     2581 attggcgttg tcgttgtcgc cgcgatgggc gaagtacgtg ccaccccacg tgccggtcgc
     2641 cgtcagcggg ccgagatagt cgaccacggc gtcatactga cgacccagcg tcaccgtgcc
     2701 gtagtcgctc gagatacccg cgaacgcctg gcggccgaag ctgcggccac tttgtgcggt
     2761 cgtgccgttg gccagactga agccgttttc caacgtgaag actgccgcgt aaccgccgcc
     2821 gagatcttca cgaccgcgca tgccccaacg gctgccagac agattgccgc tcgtcaggcc
     2881 atacgaagcg ccgtgcttcg acgcatcgct ggccgttact tcattgttca cgtaggtgaa
     2941 gccggcatcg acgacgccat agagtgtcac gctgctttgc gcattggcgg cgcaagcgaa
     3001 cgttgcggag accgccgcga gaatcaggat ctttttcatg ttgttccaat aacgtgtgat
     3061 tactgagtct tcgactcgat ttatttgcac agcgcgatgg caagctgagg acgaactgca
     3121 ccccaagcgt tcagcacacc ggctcgatat gcaggccgga gtttaggtgt ccaacttttg
     3181 gaggcaccac gcgcaatcgc acaaaatatt ttttgaatta ttcgaaaaat cgccatgaca
     3241 agatcggcaa gccttgagcc gcaaggccgt ctatttatct ccttttatat ttagctatac
     3301 ttatctaatt aaagcgttgc aacctcgcca catcacgcat taggaagcct ctcaacgcct
     3361 tttcggctgg ttcttgtggc ggttagctct tgctgcgaat accatcgctc gtttcttgcg
     3421 tgcggcgcgc gcgttagcgc ttcgcgccgt gagcctcttt tcgcaacctg cgcgcgacca
     3481 cccgacgttc gaccgaaccc ggcagctccg cgcacccagg cgcttcccgc tcaagcccga
     3541 tcaccacaat gaatcagttg caagccatgc gcgtctttct gaaagtcgcc gataacggtt
     3601 ctttcgggcg ggcagcggcg agtctggatc tctccaatgc ggtggtgaca cgctatgtcg
     3661 ccctactcga aacgcacctc gatacgcggc tcgtcaaccg caccacgcgc agcctgtcgc
     3721 tgaccgaagc cggactcgcc tatgccgagg gctgccggca actgctcgat caactggaag
     3781 cgatagagtc cagcatctct caaagtgcga acgatccgtc cggcacgctg aaactcgtgg
     3841 ctgtcgcgtc attctcattg tttgggctca cgccgctgct gcagcgttat cgtttgcagt
     3901 gcccgcgagt caaactctcg gtcaccctgc tgcatcggcc agtggatctg attgaagagg
     3961 gctttgacgt cggcatcgtg gtgcctggac aggtgagcgg cggcacgttg atcaagcgag
     4021 ccctattcaa ggtccgctca gtggcggttg cctctcccgc ctacctcgct gctcggggaa
     4081 cgccgtgcgc cccggtgcag cttgccgcac acccatttct ggcgccgtca gccaatgttc
     4141 atagcgcggc gtggtgtttc gtcgacgcca gcgaaacaga agtcaccgtc aatatggagg
     4201 ccgcctacgc ggtcaacaac gccgtgatgt tgcgccaggc ggcgctcgcc gacatgggca
     4261 ttaccatcct gccggagaat caggtttcgg cggacctgtt gagcggtgca ctcgtgcagg
     4321 tgctgccggg ctatcgaatc aaaggcgcgg acaaggaagt ctcgttggtg tatcccgggc
     4381 gccggcacgt gtccgccaag acacgatcgt ttgtggaatt caccgtcgac tacttccgaa
     4441 atcgagccgg atcgacgctt tcatcgatat tcccaaacag ttaattgcgc atttcgtgat
     4501 aaatcttttg cgcagacccg gttatgcttc cgactgcgag aagtgactcc ttcatcgagg
     4561 gatgtctcca aatctttaac gcggcttcgg ccgcgttttt tttcgtcccg ctaatgtgca
     4621 gcatggacgc cgcctggata ccaatcgagc gcgcgccgat gcgaccagcc tggcaaggcg
     4681 ctacggtcac gcttcgccac tgtgcaaggc agcgtcacgc gagcaatgca gcctgctcct
     4741 cgattccgtc cagcatcgca ttgcacttgc gcgccagcga caatccgtca cagcgcaaac
     4801 gctccggcgt cttgagggtg atgtcccgac gatagtcttc caacgcgttg agcagcggcg
     4861 ggtactgcaa cacgctcgcc gcccctgcca cacgatgatg ccactcgcgc aaacgctcga
     4921 cctcgacacg ctcgagtagc ggcgacaacg ccagcacgtc ttcgcgcacc gacgagacga
     4981 acgaatcgag caatgcctta acggtcgatt cgctccccca caactgcgtc atgtgactca
     5041 ggtcgaccgg gacgaatggc tcgggaacgc cgcgtgcatc gggtccggaa gccggcggcg
     5101 cgttgaccgt ttctaccggt gccgcttgcc atgcactgtc agtgccgaac caacggctca
     5161 ggtagtcacg caacgtggcg agccgcgtcg gcttgacgag gcagtcatcc atacctgcgt
     5221 cacgacatag cttcagatcc tccggcgcgg tgttcgcggt aattcccagg atcggcagat
     5281 ggtgcgtgtc accctgctcc tgctcccgga tgcgccgcgc cagctcgtag ccggagaggt
     5341 tgggcatgtg gcagtctgtg atcagacatc cgtagctggt ttgttcgagc gcggccagtg
     5401 cctcgacgcc gtcgttcgtc acgtcgcagg caaaaccgag cagggcgagt tgatgacgga
     5461 tcaactcctg attcaccgga tggtcttccg caaccagaat cagccgtccg ctggcaatgg
     5521 cccgttcgcg gtccggcggc acgttggcgc cttcagtcat gcgcggcatg ccggcggaac
     5581 gcggcgccac cgaaggcagc cccgtcatcg ccgcggcgca ggcggcgccg agaccgcgcc
     5641 acgagatcgg attgacactg acacgcacgt tgttgtcgag gatgcgatag ccggtcggct
     5701 tcggtttctc ggtcaggcag aggatgcgcg aaccggaccc gacgcccgcg ggcagcatca
     5761 cgtcctcgct cacgaaaatc atgtccgcgc ctgccaaggc aacggggtcg ccgagttcgg
     5821 ttgcgcctgg caacatgcac cgcaactcga gcccgagggc ctgaccgaaa tgcattagcg
     5881 cctgcgcgac ccgtgcgtca cccgtcgcca cgacgccgcg cttgccgcgc aagccgctca
     5941 ttgaataccg ctgggattcc acaggcatgg tgagacacac cgtcatgcac gtaccgaccc
     6001 caggttcgct atgcaattcg agggggccgc tcatcaggtc gatcaactta cggcagatcg
     6061 tcagccccag acccgtaccg ccgaagcggc gggtggtaga cgactccgcc tgcacgaacg
     6121 gctcgaacag ccgcgcctgc acgtccggcg ctatgccgat gccggtgtcc ttgacggtca
     6181 tctcgatagt ctgagcgtcg ccggtctgac cgacgactgc aacttgcacg tcgacctcgc
     6241 cctccggcgt gaatttgatg gcgttaccga gcagattgaa cagaatctgc cgcaatcgca
     6301 cactgtcacc acgcagcgta gccgccacat ccggcgcgat atccacgcgt accttcagcc
     6361 ctttttcgtg cgcgcggccc gcgagcaaac cgacggcatt gtcgaccagt tcgcgcatgt
     6421 cgattggctc cgcttcgatc gtcagccgcc ccgcttcgat cttcgagtaa tcgaggaggt
     6481 cgtccagaat ctgcagcaat gcacccgccg attcgtggat catgctcagc atttcccctt
     6541 gatctgcatt gagcggcgtg cgttccagca cttcgacgag gcccagcacg ccgttcatcg
     6601 gcgtacggat ttcgtgactc atcatcgcga caaattcatc tttcgcgcgc gaggcggctt
     6661 cggctacgtc gcgcgctcgc gcgagttctt cggaccgcgc gtgctctaca ctcgcgtcca
     6721 cccaatagcc gctccacact acgctgccat cctcctcgcg gcgcggcacc aactccgcgc
     6781 gcgcccactt cagttcacgc tcgacgtcga agcgaaactc catattcacc ggtgtttcac
     6841 aacgcgctga ttgctccagt tcggccagca cgaaaggccg gtcttgttcg cacacgcgtc
     6901 tgaaatcgat ctcttcgcga ctcatcgacg aagcgccgtc gccacccagc agatgccgcg
     6961 tgtcgccgcc gatatacgga aacgaatacg caccgtccgc ggtgcggcgc aattgaaata
     7021 cgacggccgg cagtgagcgt gttacgtcga aaaggcgccg ctcggtctcg cgcgcgagca
     7081 tttcagcacg ccggatatta gtaatgtcga ccatcgttcc gagcacacag ctcggcttgc
     7141 cgtcactccc atgacagatc cgtgtccaga acaagccgtg ccgcaggtcg cccgtgccac
     7201 gttcaaactg gagctccgcc tgagcgggtt cgccgtctat cagcatcttg cgtgtgagtt
     7261 cgtccagaac gcggctgttg gtttcgcccc atgtctgaat gtccatggcc gtgcgtccga
     7321 tcaccgattc gcggagcaac ccacacgctt cttcatacgc ccggttgatc gcgatgtagc
     7381 gtccatccag atccttggcg acgagcggat aggggaccat ctccatcatg gtttcctgga
     7441 aattcagctg taacgccagc tcgcgttcgg tctgtttgcg cagcctcacc tcccgctgca
     7501 acagcacata ggcgcgcagc gtgaccagca agacgagacc gatgccgatc agcaatggca
     7561 gaaggcgcac tgcggtcacg ctccatgtac ccagatctcc cgcactgccg gtcacccact
     7621 tctggcggat gcgctgcttc tcggcgggcg gcatagccag caacgcgcgg tcgatcaagc
     7681 cggcgagcgg cgccagatca gggcgcaccg cgaagccgag cgagtcggat ccgccgaccg
     7741 taccgagaat cttgagcacg cccgtgtaac gttgcttcag caggatatcg acggcggcta
     7801 cgttgccaac cagtacgtct gcctggccgt tctcgaccat cttgagcgcg tcgtccagac
     7861 tgggcgcgac ag
//
LOCUS       DATASH010000085         9766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6340402,
            whole genome shotgun sequence.
ACCESSION   DATASH010000085 DATASH010000000
VERSION     DATASH010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 9766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9766
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6340402"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1230
                     /locus_tag="VHV99_02570"
     CDS             <1..1230
                     /locus_tag="VHV99_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEX3634370.1"
                     /translation="GFTLAAYGCTTCIGNAGDLTPELNEAITKNDIVAAAVLSGNRNF
                     EARIHPNIRANFLASPPLVVAYAIAGNITRDLMTEPVGQGKGGKDIYLGDIWPTSEEV
                     NELLKFALDADAFRKNYSALTKKGDLWSKIEGEEGQVYDWPKSTYIAEPPFFGKDFSM
                     TPADSIPAVKNARALGIFGDSVTTDHISPAGSIKEDSPAGKWLKANGVQKADFNSYGS
                     RRGNHDVMMRGTFANVRIKNLMIPAKADGSRVEGGLTIHQPSGEQESIYDAAMKYIDA
                     GTPTVVFAGEEYGTGSSRDWAAKGTQLLGVKAVVARSFERIHRSNLVGMGVLPLQFKG
                     SDSVQSLGITGEETFDVEGLGNDFKPQQEVTLVIRGKDGKEKRVQVLLRIDTPIEVDY
                     YKHGGILPFVLRSLLAA"
     gene            complement(1344..2606)
                     /locus_tag="VHV99_02575"
     CDS             complement(1344..2606)
                     /locus_tag="VHV99_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634371.1"
                     /translation="MNQPQALIEGDLAFPKARPFDDLPPPFGSRRRAPRATAQNAAQR
                     EPQPAAQTDAQQAIHDAVQRKERPARQSPFPPGVKHILFYKAPPQRPDTHRFDAEMAE
                     AKRYLEMGKIRPRSSRSDYVIGATIFLGCSIALAWLLATCTTRDAQKPTTLAVTQPVV
                     PAVTGAPNDHLQEPGKLAQLAVEDAQTVAQSASSVASAVPAASAPSSVAAVTASASSA
                     TSPVAATVAGSARSKASSAVAVVAPASSVTSYAAADVALASSEASYAVPTASPASRDR
                     NDGAAPVAAPGKPRTQLSQHLTPQPTMRASARETTTGRPAKVERKLVVARMTEAQVDE
                     RLAVSRTVRPATRPSVSKQPEWTARPSAETDAIDQAALLNWAAQQRAHVTTRATLSAP
                     GDTDWNARMTQRRITDNPDAFQAGRGQK"
     gene            complement(2603..2992)
                     /locus_tag="VHV99_02580"
     CDS             complement(2603..2992)
                     /locus_tag="VHV99_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEX3634372.1"
                     /translation="MFALAARLQDAAASTPSEPFERRYLDALSEEGIDLQTFLGGWRH
                     SMDEDLKRLSGASYERDREGLSNLLHRLSGAVGLVGARSLMEALRHASTTPLEHEAGA
                     IDALMERARTLVAQLDTAVEPHRSATP"
     gene            complement(3018..4205)
                     /locus_tag="VHV99_02585"
     CDS             complement(3018..4205)
                     /locus_tag="VHV99_02585"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX3634373.1"
                     /translation="MGCLFAFALLCLLSIRANAAPLVDSTPHALRVDDARPAAVLAAR
                     DAVGQLRQSASDFHSREETTASAFLALAPADNRNAFGQPATGARDATIERQTWLLALA
                     APAVVALAGACLSRAALRRAAEKLHHAENEWTRRCAAAEAREQAARVAASAEASAAAK
                     QERLWLLGAMRLYAEAPLTAVTGLLASLETAATPSAQRAQMPVIQSAVHTWGQTLHDL
                     LDVSPLESRDFVLDESATNLRELVDGVIALLTPSAAQRGLRLSSSIDQTVAERILADS
                     ARLGQIFFHLLNRTIQASTRGEIALVVRAEPSNSGSQRMVISVTDAGEKAAHTAQLQL
                     FGLSADETSAGERLGDADACLPLCQILAQRMQGELFIASSSDSGTRGRFSAPFTVDQW
                     GRH"
     gene            complement(4344..4979)
                     /locus_tag="VHV99_02590"
     CDS             complement(4344..4979)
                     /locus_tag="VHV99_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3634374.1"
                     /translation="MSTILVIDDHPAFRLVMKMQLMQLLGVEEVIEADNGQTAVEMAR
                     QHTPKLAILDLDIPRISGLDVIPRLKLAHPAMRVLVLSGQEPATFAPRAMRSGVHGFV
                     AKSQEMKEIMRGVEAVLAGYTVFPVTASGAGIARGANVANEEDRLTQLSDKELVILQM
                     LSKGMSNKAIGDALFMSDKTVSTYKSRIQEKLGLSSLAALIEFATLHRLID"
     gene            complement(5220..6281)
                     /locus_tag="VHV99_02595"
     CDS             complement(5220..6281)
                     /locus_tag="VHV99_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX3634375.1"
                     /translation="MKLQLTILACALALSGCADHAARERLGIEDATVLKTGVGAAQDE
                     AAGAVNGQWIDTYAPIVSSRTALTSLQQRLDQVPGDKSSYFHAKAQCWLDAGQQASQA
                     SDHWGFVEEAIGQAAMITMSLENGSPLSAANPTLRTVSTVRPDLWKIVNTIKSDPAIA
                     QCPEAQRPLACAEVELTQAGHDAWTRSFSRAEKRLPEVQDNLRKSAETALQCSQAKHA
                     VAPAPAAQAPRKITLRADSLFRFNGADEAAILPAGKQHLDAVVTGLKKVPAIRELKIT
                     GYADRLGSEVYNQSLSLQRAQTVQQYLSNHGVTLPVTAQGQGSANQLVTCQQTKRDEL
                     VRCLAPNRRVEIEFVLGAS"
     gene            complement(6606..7445)
                     /locus_tag="VHV99_02600"
     CDS             complement(6606..7445)
                     /locus_tag="VHV99_02600"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3634376.1"
                     /translation="MKLAKPLFAPHTSLVERVWYFALRALAVLTLLYLILPVLAIVPL
                     SFSSSTFLVYPIPGWSLRWYQNLIASDEWRMAAKNSFIVAPSATVVATVLGTLAAIGL
                     TKANFRGKALLMAILISPMIVPVVVVGVGMYLFFAPLGLANTYIGLILAHASLGVPFV
                     VTTVAATLQGFNYNLVRASLSLGANPVKTFFRITLPVIAPGVISGALFAFATSFDEVV
                     VTLFLAGADQTTLPRQMFTGIRENISPTIAALATILIVFSTCLLLALEWLRGRNAARA
                     VKA"
     gene            complement(7470..8747)
                     /locus_tag="VHV99_02605"
     CDS             complement(7470..8747)
                     /locus_tag="VHV99_02605"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004190905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3634377.1"
                     /translation="MTTMTIAADSTPESSVRLKRELKAAEAKKRAMALLLIAPLAIFL
                     LLIFVVPIGALLTRAAQNPEVVNALPHTLHALRDWDRKTLPPDAAYAALAVDLAAVAD
                     SDGMGALARRLNVEIPGYRSLVAKSAHAMPFVDDNSKPLNLAPAQVHAKFVELDERWN
                     DITYWQAIAKNSGVYSPFYLLASLDHKQDAFGHIMPTDPDQQIYLAVFSRTFVIGAVV
                     TFFTLLLGYPLAYWISTLSDRRANLVMILVLIPFWTSILVRVAAWIVILQSEGLVNKA
                     LIASGLLHDPLSLLFNRTGVYISMTHILLPFMILPLYSVMKSIPPTYQRAAISLGSHP
                     FAAFWRVYVPQTYPGIGAGALLVFILAIGYYITPALLGGPGDQMVSYYVAYFTNVTIN
                     WGMACALGGLLLAATLVLYVIYGRFTRSSLSLG"
     gene            complement(8978..>9766)
                     /locus_tag="VHV99_02610"
     CDS             complement(8978..>9766)
                     /locus_tag="VHV99_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004549588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3634378.1"
                     /translation="ESGDIGRGCDEGLYEKLDWSKIGKKSDLIPEAPQACGVGFFVWS
                     TALSYNADKLKTAPTGWADFWDVKKFPGKRAMRKGARYNLEFALMADGVPPKDVYKVL
                     ATKEGQDRAFKKLDELKPNIQWWEAGAQPPQFLVAGDVVMSTAYNGRIDAAQKEGKNL
                     KVVWNGSIYDLDYWAIPKGSPNKALAEKYIAYSLSSKPQQDYAHNIAYGPVNVAAIKA
                     LDPKTLANLPNSPANGKNAVLQNLAFWTDHGDELEQRFSSWASK"
BASE COUNT         1966 a         3026 c         2902 g         1872 t
ORIGIN      
        1 ggtttcacgc tggccgctta tggttgcacg acctgtatcg gtaacgcggg cgacctgacg
       61 ccggaactga acgaagcgat cacgaagaac gacatcgtcg cggcagctgt tctgtcgggc
      121 aaccgtaact tcgaagcgcg tattcacccg aacatccgcg ccaacttcct ggcttcgccg
      181 ccgctggtcg tcgcatacgc gatcgccggc aacatcacgc gcgacctgat gaccgaaccg
      241 gtcggccagg gcaagggcgg caaggacatc tacctgggcg acatttggcc gaccagcgaa
      301 gaagtcaacg aactgctcaa gttcgcgctg gacgcggatg cgttccgcaa gaactactcg
      361 gcactgacca agaagggcga cctgtggagc aagatcgaag gcgaagaagg tcaagtctac
      421 gactggccga agtcgaccta catcgctgag ccgccgttct tcggcaagga cttctcgatg
      481 acgccggcag acagcatccc ggccgtcaag aatgcgcgcg cactgggcat cttcggtgac
      541 tcggtcacga ccgaccacat cagcccggct ggctcgatca aggaagactc gccggcaggc
      601 aagtggctga aggcaaacgg cgtgcagaag gccgacttca acagctacgg ctcgcgccgc
      661 ggcaaccacg acgtgatgat gcgcggcacg ttcgcgaacg tccggatcaa gaacctgatg
      721 atcccggcga aggcagacgg ctcgcgcgtg gaaggcggcc tgacgattca ccagccgagc
      781 ggtgagcagg agtcgatcta tgacgccgcg atgaagtaca tcgacgcggg tacgccgact
      841 gtcgtgttcg cgggtgaaga gtacggtacg ggttcgtcgc gtgactgggc ggccaagggt
      901 acgcaactgc tgggcgtgaa ggccgtcgtt gcgcgtagct tcgagcggat ccaccgttcg
      961 aacctcgtcg gcatgggcgt gctgccgctg cagttcaagg gctcggacag cgtgcaatcg
     1021 ctcggtatca ccggcgaaga aacgtttgac gtcgaaggcc tcggcaacga cttcaagccg
     1081 cagcaggaag tgacgctggt gattcgcggt aaggacggta aggagaagcg cgtgcaggtt
     1141 ctgctgcgta tcgacacgcc gatcgaagtc gactactaca agcacggcgg cattctgccg
     1201 ttcgtgttgc gttcactgct ggctgcttaa gttattcgct tacgcatgca gtgactgcga
     1261 gctgttttgc aggggctgcg tcctgtggag gacgcagccg attttggaag cccggctttt
     1321 ggccgggctt tttttatgac tttttacttt tgaccgcggc ctgcctggaa ggcgtccggg
     1381 ttgtcagtga tccggcgttg ggtcatgcgg gcgttccaat cggtgtctcc gggtgccgac
     1441 agcgttgcac gcgtcgtgac gtgcgcgcgc tgctgggcgg cccagttgag caacgctgcc
     1501 tggtcgatcg cgtcggtttc agccgacgga cgtgcggtcc actcaggttg cttcgacacg
     1561 gaaggcctcg tggctggacg aacggtgcgg ctcaccgcga gccgctcgtc gacctgcgcc
     1621 tcggtcatgc gtgcgacgac gagtttgcgt tccacctttg ccggacggcc cgtggtggtt
     1681 tcacgagcag acgctcgcat ggtcggctgc ggcgtgaggt gctgcgacag ctgcgtgcgc
     1741 ggttttccag gggcggccac aggggcagcg ccatcgttcc tgtcgcggct cgctggtgac
     1801 gccgttggca ctgcatagct tgcctcggaa gacgctagcg cgacatctgc cgctgcataa
     1861 ctcgtgaccg agcttgccgg cgccacgacc gccacagcag agcttgcctt cgaacgcgca
     1921 gaccctgcca cagttgccgc cacagggctc gtcgccgagc ttgcggatgc cgtcactgcc
     1981 gcaacggaac tcggcgcgga tgcggccggt accgcactcg cgaccgaaga cgcgctctgc
     2041 gcaaccgtct gcgcgtcctc aaccgccaac tgcgccagct tgccgggctc ttgcaaatga
     2101 tcgttgggtg cgcccgtcac cgcaggcaca accggctgcg tcactgccag cgttgtcggc
     2161 ttctgcgcgt ctcgcgtcgt gcatgttgcc agcaaccagg ccagcgcgat gctgcaaccc
     2221 aggaagatcg tcgccccgat tacgtaatcc gagcgcgacg agcgcggacg gatcttgccc
     2281 atctcaaggt agcgcttcgc ttcagccatc tccgcatcga aacgatgggt atccgggcgt
     2341 tgcgggggcg ccttgtagaa cagaatgtgc ttcacgcccg gaggaaacgg gctttggcgc
     2401 gccggccgct ccttgcgttg caccgcatcg tggatcgcct gttgcgcgtc agtttgtgct
     2461 gcgggttggg gttcgcgttg cgccgcattt tgtgctgtgg cccgcggcgc acgtcgtctc
     2521 gagccgaaag gcggcggcaa gtcatcaaat ggccgcgcct tcgggaacgc cagatcgcct
     2581 tcgatcaagg cctgcggttg gttcatggtg tggcgctcct gtggggctcg accgctgtgt
     2641 cgagttgcgc aaccaaggtc ctggcgcgct ccatgagcgc gtcaattgca cccgcctcgt
     2701 gctccagcgg ggtcgtgctg gcgtgccgca gtgcttccat tagactgcgc gcacccacca
     2761 aacctacggc tccgctcaat ctgtgcagca ggttgctcaa tccttcccgg tcgcgctcgt
     2821 aactcgcgcc gctcaggcgc ttcaggtctt cgtccatcga atggcgccag ccgcccagaa
     2881 acgtctggag atcgatgccc tcttcagaca gcgcgtcgag atagcggcgc tcgaagggtt
     2941 cagatggggt actcgcggct gcatcctgca accgcgcggc aagggcgaac aagggcgcct
     3001 gcgcatgacg agccggttca gtgacggccc cattgatcaa cggtgaacgg tgcactgaac
     3061 ctcccgcgag tgccggaatc tgaactgctt gcgatgaaaa gctcgccttg catacgctgc
     3121 gcgaggatct ggcaaagcgg caagcaagca tcagcgtcgc caagtcgttc tcccgcggac
     3181 gtttcatcag cgctcagccc gaaaagttgc agttgtgcgg tgtgcgcggc tttttcacca
     3241 gcgtccgtca cgctaataac catcctctgt gagccggaat tagacggctc cgcccgcacg
     3301 acgagcgcaa tctctccacg cgtgctagcc tggatcgtgc ggttcagcag atggaagaag
     3361 atctgaccga gccgggcact gtcggcgaga atccgctcgg cgaccgtttg gtcgatgctc
     3421 gaactcaggc gcaagcctcg ctgagccgca gacggggtca acagtgcaat gacgccgtcg
     3481 acgagttcgc gcagattcgt cgcgctttcg tcgagaacga agtcacgcga ttccagtggc
     3541 gaaacatcca gcaagtcgtg aagcgtttga ccccatgtgt gcaccgcgga ttggatcacc
     3601 ggcatctgcg cgcgttgagc cgacggtgtg gccgccgttt cgagcgatgc aagaagaccc
     3661 gtcacggcgg tcaatggtgc ttccgcatag agacgcatcg cgccgagcaa ccaaagccgt
     3721 tcctgcttcg cggcagcgct cgcctcagcg gatgctgcga cgcgcgcggc ctgctcgcgc
     3781 gcttcggctg cagcgcagcg gcgcgtccat tcattctcag cgtggtgcag tttctcagcg
     3841 gcgcgtctca gcgcggcgcg agacaggcat gcgccggcca acgcaacgac cgccggcgcc
     3901 gcgagcgcca acagccacgt ttgccgctcg atggtggcat cacgtgcgcc tgtcgccggt
     3961 tggccgaacg cattgcggtt atcggcggga gcaagcgcaa gaaacgcgct agcagtggtc
     4021 tcctcgcgcg aatgaaaatc gctggccgac tgcctgagtt gtcccactgc gtcgcgcgcg
     4081 gcgagcactg cggccggacg tgcatcgtca acgcgcaatg cgtgcggtgt actgtcgact
     4141 aacggcgccg cgttggcgcg tatcgagagc aggcacagca atgcaaacgc aaagaggcag
     4201 cccaataccg tcgcaaaacg gatcgaagtc agcccggtgt tgtgagagag actgtgagaa
     4261 aaattgtaag aggagcgcgg cgcacgacga cgcgcgcccg ctgaagaacc aggcaggctc
     4321 attttgttca tagcccgcaa cattcagtcg atcagcctgt gcagcgtagc aaactcaatc
     4381 agcgcggcca atgacgacag tccaagcttt tcctggatgc ggctcttgta tgtgctcact
     4441 gtcttgtcgc tcataaacag cgcgtcgcca atcgccttgt tcgacatgcc tttcgacagc
     4501 atctgcaaga tcactagctc cttatcggac aattgcgtga gtctgtcctc ttcatttgcc
     4561 acattagcgc cacgcgcgat accggccccg ctggccgtca cggggaacac cgtatagccc
     4621 gccagtacag cctcgacacc gcgcatgatc tctttcattt cctgcgactt ggcgacaaaa
     4681 ccgtgtacac ccgagcgcat cgcccgtggc gcgaaggtgg ccggctcctg cccggacagc
     4741 accagcacgc gcatcgccgg atgcgccagc ttgagacgcg gaatgacatc gagcccgctg
     4801 atgcgcggaa tgtcgagatc gaggatcgca agtttcggcg tgtgttgtcg ggccatctcg
     4861 acagcggttt gtccgttgtc ggcttcgatt acttcttcaa ctccaagcag ttgcatgagt
     4921 tgcatcttca tgaccagccg aaatgcgggg tgatcgtcga taaccagaat agtagacatt
     4981 tataacattc ctcatgaaat cattcccttg ccatgccggc gcttaccgcc agccatagaa
     5041 gccgcatcta acgagaaaaa tgcgcgcaac gtccccacct ctcggcgcaa gactgcaagg
     5101 gattaggttt aggatctcgt agcaaatccg tgatacaacg cgggctagcc gaaatatcga
     5161 ccggccgcac gaagtcggca ttcgttcacg ccgacgatca ttcttgatgc gccggcaagc
     5221 taggaagccc cgagcacgaa ctcgatctcg acgcggcgat ttggtgcaag acatcgcacc
     5281 agttcgtcgc gtttcgtttg ttggcaagtc accagttgat tcgcactgcc ttgcccctgt
     5341 gcggttaccg gcaaggtcac gccgtgattg ctcagatact gctggaccgt ctgtgcacgc
     5401 tgcaacgaaa ggctctgatt gtaaacttcg ctgccaaggc gatcggcgta gccggtgatc
     5461 ttcaactcac gtatcgcggg aaccttctta agacccgtca ccacggcgtc gagatgttgc
     5521 ttgccagccg gcagaatcgc cgcctcgtcg gccccgttga aacggaacag cgaatcagct
     5581 cgcagcgtta tctttcgcgg cgcctgcgcc gcaggtgcgg gtgccaccgc gtgcttcgct
     5641 tgcgaacatt gcagcgccgt ttccgctgat ttccgcaggt tgtcctgaac ttccggtaag
     5701 cgcttttcag ctctggagaa actccgtgtc catgcgtcat gtccagcttg cgtcagttcg
     5761 acttcggcac acgcaagagg ccgttgcgct tcagggcatt gggcgatggc aggatcggac
     5821 ttgatagtgt tgacgatctt ccacagatca gggcgcactg tcgacacggt gcgcagagtc
     5881 gggttagctg cggacaacgg cgagccattt tcaaggctca tcgtaatcat cgcagcctgg
     5941 ccgattgctt cctcgacgaa gccccagtga tcgctggcct gcgacgcctg ttggccagcg
     6001 tctaaccagc attgcgcttt cgcgtggaaa tagctgcttt tgtcgcccgg tacttgatcg
     6061 agacgttgtt gcaacgatgt cagcgcggtg cggctgctga cgatcggcgc gtaagtgtcg
     6121 atccattgcc cgttgaccgc gcctgcggcc tcgtcctgcg cggcgccgac gccggttttc
     6181 agcacggtgg catcttcgat gccgagccgc tcacgcgcag catggtccgc acagcctgac
     6241 aaggccagcg cacatgcgag gatcgttaac tggagtttca ttggatttct gcgtcgaacg
     6301 catcttcttt aagatggttc tgagtatgaa gagacgcgga tgcaggggga atgggaaaat
     6361 tcttagaatg acgtcagaaa gttgagactt tgggaagttc acgtcaaacg caggaaaagg
     6421 tccgtatcgg agaaggtcgg gacgtttacg tgagcccgcg atgtctccgt aacgcaaggt
     6481 cccgcaccaa cgatcaatgt tgtgctgcac gtgtcgcgcc cataggcatt atgtgcgcga
     6541 cggataatcc agccagcctc gcactctctt gccacataac ggctgtcgtt gatacggtct
     6601 gcccctcacg ccttcaccgc ccgcgcggca tttcgccccc gcagccattc cagtgccagc
     6661 agcaggcaag tcgaaaacac aatcaaaatt gtcgccagcg ccgcaatcgt cgggctgatg
     6721 ttctcgcgaa tgccagtgaa catttgccgc ggcagcgtcg tctgatccgc gcctgcgaga
     6781 aacagcgtca ccacgacctc atcgaacgat gtcgcgaacg cgaacaatgc ccccgagatc
     6841 acacccggcg caatcaccgg caacgtaatg cggaaaaacg tcttcaccgg atttgcgccc
     6901 agcgacaagc tggcgcgcac caggttgtaa ttgaagcctt gcaacgtcgc ggccaccgtc
     6961 gtcacgacaa acggcacacc aagcgacgca tgcgccagaa tcagcccgat ataagtattc
     7021 gctagcccca gcggcgcaaa gaacagatac atgccaacgc cgaccaccac cacgggcacg
     7081 atcatcggcg aaatcagaat cgccatcagc agcgccttgc cgcggaagtt cgctttggtc
     7141 agcccgattg cagcaagcgt gccgagaaca gtcgcgacca cagtcgccga cggcgccacg
     7201 ataaagctgt tcttcgccgc catgcgccac tcatcggacg cgatcagatt ctgataccag
     7261 cgcagcgacc agcccggaat cggatacacc agaaacgtgc tcgacgaaaa cgacagcggc
     7321 acaatcgcca gcaccggcaa gatcagatat agcaacgtca gcacggcaag cgcgcgcagt
     7381 gcgaagtacc acacgcgttc aaccaacgac gtatgcggtg cgaacaaggg cttggcaagt
     7441 ttcatcgcaa gtaggctccg ttcagccaat cagccgaggc tcaaggacga acgcgtaaaa
     7501 cgcccgtaga tgacgtacag cacgagcgtc gcggcgagca gtaagccgcc gagcgcgcac
     7561 gccatacccc aattgatcgt cacgttcgtg aaatacgcga cgtagtagct gaccatctga
     7621 tcgcccgggc cgccgagcaa tgctggcgtg atgtagtagc cgatcgccag aatgaacacg
     7681 agcagtgcgc ctgcgccgat gcccggataa gtctgcggca cgtacacgcg ccagaacgcc
     7741 gcgaacggat ggctgcccag cgaaatcgcg gcgcgctgat aggtcggcgg gatcgacttc
     7801 atcacgctat acagcggcag gatcatgaac ggcagcagaa tgtgcgtcat cgagatgtag
     7861 acgccggtgc ggttgaacag cagcgacagc gggtcatgca acagcccgct cgcaatcagc
     7921 gccttgttca ccagcccctc gctttgcaga atcacaatcc acgcggcgac gcgcacgagg
     7981 atcgacgtcc agaacggaat cagcacgaga atcatcacca gattcgcgcg acgatcggac
     8041 agcgtcgaga tccagtacgc gagcggataa ccgagcagca gcgtgaaaaa cgtcacgacc
     8101 gcaccgatga caaacgtgcg gctaaacaca gcgagataga tctgttggtc tggatcggtc
     8161 ggcatgatgt ggccgaacgc gtcctgtttg tggtccaacg aagccagcag atagaacggt
     8221 gaatacacac ctgaattctt ggcaattgcc tgccagtagg taatgtcgtt ccagcgctcg
     8281 tcgagttcga cgaacttcgc gtgaacctgg gcgggcgcga gattcaatgg cttgctgttg
     8341 tcgtcgacga acggcatcgc gtgcgccgac ttcgctacca gagagcggta gccgggaatt
     8401 tcgacgttca agcggcgtgc cagcgcaccc atgccgtcgc tatcggcgac tgcggcgagg
     8461 tccaccgcca atgcggcata cgccgcgtca ggaggtagcg tcttgcgatc ccaatcgcgt
     8521 aatgcatgca gcgtatgcgg caaggcgtta acaacttccg gattctgcgc ggcgcgcgtc
     8581 agcagcgcac cgattggcac gacgaaaata agtagcagaa aaatggcgag cggtgcgatc
     8641 agtaggagcg ccatcgcgcg ttttttcgct tccgcggcct tcagttctcg cttgagccga
     8701 acactcgact cgggcgtcga atcagcggcg atcgtcatcg tagtcacacc cgtctccatt
     8761 gtcgacggga gttagcgctt tagcgcttac tcctgtccca cgtaccagcg agtcaacagg
     8821 agttagcgct ttagcactca cccccgtccc acgcaccaac agtcaatccc acgcatccag
     8881 ctgcgcgcgc acatcgttca gataggcgcg cgtcgtcttg catcgatacg gcacgacttc
     8941 actaacaaag aaaccgcgcc gcctcaccca aacctgctta cttcgaagcc cacgacgaaa
     9001 accgttgctc caactcatcg ccatggtcgg tccagaacgc gagattctgc agcacagcat
     9061 tcttgccatt agccggcgag ttcggcagat tcgcaagcgt cttcggatcg agcgccttga
     9121 tcgccgccac attcaccgga ccgtacgcga tgttatgcgc gtagtcctgc tgcggcttcg
     9181 acgaaagcga atacgcgatg tacttctcag ccagcgcctt attgggcgag cccttcggaa
     9241 tcgcccaata gtcgaggtcg taaatgctgc cgttccacac caccttgagg ttcttaccct
     9301 ccttctgcgc ggcatcgata cggccgttgt acgcggtcga catcaccaca tcacccgcga
     9361 cgagaaattg cggcggctgt gcgcccgctt cccaccattg aatattcggc ttcagttcgt
     9421 cgagcttctt gaacgcgcgg tcctggcctt ccttggtggc gagcaccttg tagacgtcct
     9481 tcggcggcac gccgtcggcc atcagcgcga attccaggtt gtaccgcgcg cctttgcgca
     9541 tcgcacgctt gccggggaat ttctttacgt cccagaagtc ggcccagccc gttggcgcgg
     9601 ttttcagctt gtcggcgtta tacgaaagtg ccgtcgacca cacgaagaaa cctacgccgc
     9661 aagcttgcgg cgcttccgga atcagatccg attttttgcc gatcttcgac cagtcgagct
     9721 tctcgtacaa cccttcatca caacctcggc cgatgtcgcc cgattc
//
LOCUS       DATASH010000086         3048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3571276,
            whole genome shotgun sequence.
ACCESSION   DATASH010000086 DATASH010000000
VERSION     DATASH010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3571276"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..685)
                     /gene="queE"
                     /locus_tag="VHV99_02615"
     CDS             complement(53..685)
                     /gene="queE"
                     /locus_tag="VHV99_02615"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007733358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase"
                     /protein_id="HEX3634379.1"
                     /translation="MTYAVKEIFYTLQGEGANAGRPAVFCRFAGCNLWSGREEDRAEA
                     VCRFCDTDFVGTDGENGGKYRTAEELVRMIASQWPEGEGERFVVCTGGEPMLQIDQPL
                     VDALHAAGFEIAIETNGSLPVLETIDWICVSPKADAPLVVTKGNELKVVIPQDNQRLS
                     EYAKLDFEYFLVQPMDGPSRDLNTKLAIDWCKRHPQWRLSMQTHKYLNIP"
     gene            complement(773..1504)
                     /gene="queC"
                     /locus_tag="VHV99_02620"
     CDS             complement(773..1504)
                     /gene="queC"
                     /locus_tag="VHV99_02620"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEX3634380.1"
                     /translation="MIRKDAKRSALVLFSGGQDSATCLAWALERYETVETLGFDYGQR
                     HRVELECREGFRNAVVRAFPAWADRLGEDHMIDLSVLGSISDTAMTREIEIQATANGL
                     PNTFVPGRNLMFMTIAAAIAYRRGLQVLVGGMCETDFSGYPDCRDDTMKALQVALNLG
                     MDTRFLLETPLMWLDKADTWRLAHELGGDELVELVRVETHTCYVGERAELHAWGFGCG
                     ECPACRLRKRGYEAYLAGEKVTEPV"
     gene            complement(1944..2019)
                     /locus_tag="VHV99_02625"
     tRNA            complement(1944..2019)
                     /locus_tag="VHV99_02625"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1984..1986),aa:Phe,seq:gaa)
     gene            complement(2116..2808)
                     /gene="esaR"
                     /locus_tag="VHV99_02630"
     CDS             complement(2116..2808)
                     /gene="esaR"
                     /locus_tag="VHV99_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor EsaR"
                     /protein_id="HEX3634381.1"
                     /translation="MATILVVDDEMGIRELLSEILSDEGHVVEAAENAQEAREFRLRQ
                     APDLVLLDIWMPDTDGVTLLKEWAAQGQLTMPVIMMSGHATIDTAVEATKIGALNVLE
                     KPIALQKLLKAVEQGLARGTAPAGAPGGAAAKPAVPVSASAVASAAALPMLSSDGIGS
                     GALAAQTASISFDIPLRDARDAFERAYFEYHLARENGSMTRVAEKTGLERTHLYRKLK
                     QLGVDLGKNKGE"
     gene            complement(2809..>3048)
                     /locus_tag="VHV99_02635"
     CDS             complement(2809..>3048)
                     /locus_tag="VHV99_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634382.1"
                     /translation="KAKGTGLGLAMVKKIVDEHGARIDVRNRMKAGDVIEGAQISILF
                     LQLADGAATPGAGTRPAHGNASQGTTKATVQTRAA"
BASE COUNT          570 a         1007 c          882 g          589 t
ORIGIN      
        1 tgcggtggcc cgcgtcgaat tcgagttttc gtgtaatcgt cagcacggca aatcagggaa
       61 tgttcagata cttgtgggtc tgcatcgaca ggcgccattg cggatggcgt ttgcaccagt
      121 cgatcgcgag cttggtgttg agatcgcgcg acgggccgtc cattggctgg acgaggaaat
      181 actcgaagtc gagcttcgca tactcggaca agcgctggtt gtcctgcgga atcacgacct
      241 tcaattcgtt acccttggtg acgacgagcg gcgcgtcggc cttcgggctc acacagatcc
      301 agtcgatcgt ttcgagcacc ggcagcgagc cgttggtctc gatggcgatt tcaaagccgg
      361 cggcatgcag tgcgtcgacc aagggctgat cgatctgtag catcggttca ccgcccgtgc
      421 acaccacgaa gcgctcgccc tcgccttccg gccattgcga cgcgatcatc cgcaccagct
      481 cttcagccgt gcggtacttg ccgccgttct cgccgtcagt gccgacgaag tccgtatcgc
      541 agaaacggca cacagcctcc gcacgatcct cttcgcggcc cgaccacaga ttgcagccgg
      601 cgaaccggca gaacacggcc ggacgcccgg cattcgcgcc ctcgccctgc aatgtgtaga
      661 agatttcctt gaccgcgtaa gtcatgctgc tttctgcctt gttcctaaaa atagttgcct
      721 gcgctgcgcg ccgttcgtac gatgcgccag cgtaaaacct gtcatgagac ggtcaaaccg
      781 gctcggtcac cttctcgccg gccagatacg cttcatagcc gcgcttgcgc aaacggcacg
      841 ccggacattc gccgcagccg aagccccatg catgcaattc ggcccgctcg ccaacatagc
      901 aggtgtgcgt ttcgacgcgc accagttcga ccagttcgtc gccgcccagc tcgtgcgcga
      961 gacgccacgt gtcggccttg tcgagccaca tcagcggtgt ttcgagcagg aagcgcgtat
     1021 ccatgccgag gttcagcgcg acctgcaatg ccttcatggt gtcgtcgcgg caatcggggt
     1081 agcccgagaa atccgtctcg cacatgccgc ccaccagcac ctgcaaacca cgccgatatg
     1141 cgatggcggc ggcaattgtc atgaacatca gatttcggcc cggcacgaac gtattcggca
     1201 agccgttcgc ggtggcctgg atctcgatct cgcgcgtcat tgccgtatcg ctgatcgaac
     1261 ccagcaccga gaggtcgatc atgtgatctt cgccgaggcg atcggcccac gccggaaaag
     1321 cgcgtaccac agcgttacga aacccttcgc ggcattcgag ttcgacgcgg tgccgctgac
     1381 cgtaatcgaa gcccagcgtc tcgaccgttt cataacgttc gagcgcccag gcgaggcacg
     1441 tggcggaatc ctggccgccg gaaaacagca ccagtgcgct acgtttagcg tctttgcgga
     1501 tcaccgtgaa actccgtgaa tgaagagtgc cacgtcgcgc tgcgcgaggt gcgccatgca
     1561 agcaacgcgg cgcgacgcgt gccggcactg ctgccggccc aaagcagtat aggccgcgcc
     1621 ttctgaacgc agagtcgctg ggcgcttata ccgttaatcg tcgggtgaag ggttcggcgc
     1681 ctgttgcagc cttgccgcgt tgcggcagag tgcgcaaaag gcccagcttc aggatgcgaa
     1741 aagacgtgtt cggtgcggtg acgcgccagc cggacgtcgg ccacgcactc gcatgcaagt
     1801 ccttgaactg gcgcgatttt atcacgcgat gcctaatgcc gccgagcatg ccccactaca
     1861 gcggcaagca agccatgcca ccacgaacgc ccgcctgaaa aggaaaagcc ccaggaatct
     1921 tgcgatttcc tggggctgaa tcctggtggc ctgggacgga atcgaaccat cgacacgcgg
     1981 attttcaatc cgctgctcta ccaactgagc taccgggcca acgaagaacg aaatgatatc
     2041 aaagggtcag cagtacgaca agcccccctc tcgaaaaaat ctgccgcgcc cgaacccgcg
     2101 ggccttgcgc ggcgttcact cccctttgtt cttgccgaga tccacgccga gttgcttgag
     2161 cttgcgatac aagtgcgtgc gctccagacc cgtcttttcc gcgacgcgcg tcatgctgcc
     2221 attctcacgc gcgaggtgat attcgaagta cgcacgctcg aacgcatcgc gcgcatcgcg
     2281 caacggaatg tcgaacgaaa tcgacgcggt ttgcgcagcc aacgcgccgc tgccgatgcc
     2341 gtccgacgac agcatcggca acgcagccgc cgatgccacc gccgaagcgc tgaccggcac
     2401 cgccggcttc gccgccgcgc cgcccggcgc accagccggc gcagtgccgc gggcgaggcc
     2461 ttgctcgact gccttcagca gcttctgcaa cgcaatcggc ttctcgagga cattgagcgc
     2521 gccgatcttg gtcgcttcga ccgccgtatc gatggttgcg tgaccggaca tcatgatcac
     2581 cggcatggtc agttgccctt gcgcggccca ttccttgagc aaggtcacgc cgtcggtatc
     2641 gggcatccag atgtcgagca gcaccaggtc cggcgcctga cgcaagcgga actcgcgcgc
     2701 ttcctgcgcg ttttccgcgg cctccacgac atgcccttcg tcgctcagga tctccgagag
     2761 caattcccgg atgcccattt catcatctac caccaggatg gttgccattt acgctgccct
     2821 tgtctgcact gttgcttttg tcgttccctg cgacgcgttg ccatgcgccg gccgcgtccc
     2881 tgctccgggt gtcgcggcac cgtctgccag ttgaaggaag aggatcgaaa tctgcgcgcc
     2941 ttcgatcaca tcgcccgctt tcatgcggtt gcgaacgtcg atgcgcgcgc cgtgttcgtc
     3001 gacgatcttc ttgaccatcg caagaccgag gcccgttcct ttggcctt
//
LOCUS       DATASH010000087        12535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1593762,
            whole genome shotgun sequence.
ACCESSION   DATASH010000087 DATASH010000000
VERSION     DATASH010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 12535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12535
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1593762"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="VHV99_02640"
     CDS             complement(<1..756)
                     /locus_tag="VHV99_02640"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX3634383.1"
                     /translation="MFARVSVHSRHAVLMIHGLGGTRHDFGALDRKFEQIGCDVHLPS
                     LPGHGSNPDALNRVTLSDYMQLLSRTYRDLMARYERVDVAGISMGALLALMLSARERM
                     TTGRLILLSPPLFLDGWGGSRLRPLRYLLYCVPGLRRMIRVPEGEPFGIKNARIRNLI
                     RRHLRKGGGSHYPYVPLAAIEQVDWMRFAVKRALSRVTCETLVMHSEEDEVTSIRSAE
                     LVCEHLGSRSVTFVRLTDSYHMITLDNERDTVAE"
     gene            complement(938..1642)
                     /locus_tag="VHV99_02645"
     CDS             complement(938..1642)
                     /locus_tag="VHV99_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004184573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3634384.1"
                     /translation="MPFRILLVEDDDRLSALIAGYLRKHEYEVETVLHGNGAVPAILK
                     RRPDLVILDVNLPGKDGFQICREAREQFDGVIIMVTARDEQFDEVLGLEFGADDYVHK
                     PVEPRVLLARIKAQLRRVTARTPEAAQPESYICGRFEISRASRTVKLPDGSLPDLTSA
                     EFDLLWALVSCAGEVVSRDELMRQLRGIEFDGLDRTIDGGISKLRRKLHDDAGTPQRI
                     KTVRGKGYQFNKQAWD"
     gene            1899..2963
                     /locus_tag="VHV99_02650"
     CDS             1899..2963
                     /locus_tag="VHV99_02650"
                     /inference="COORDINATES: protein motif:HMM:NF016293.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 family protein"
                     /protein_id="HEX3634385.1"
                     /translation="MTTVSNERARARLSWFARAAASFAVIQQAAHARYPGNGAFHRWR
                     RLRLWLRSLMSWHSTQVWLKRCAISPLSDLVQRHPTALERVHRPFLHTRFSPRERLTA
                     SLDHHALTQQRAPQLAARIAAVGSAPIASFGVGAERWYVSLESIEQFQKEGDWTLCIR
                     DARDQRIVSCTFSLAYLGGKVRRPRLCIGSVQGPDSSVNGRELFRALTRRWYGLRPKA
                     LVIYLAQCIAAALDAGGTFIVSRQAHIYANWRYCLRKRRVAADYDRLSIECGALARWN
                     GWFVLAPPARYLAERQGDDTGSATRRKRYALRSELAAQIRRSIAAGNTQTQHASCCHL
                     NPLRFFIDKISGKTAHSFAC"
     gene            3149..3529
                     /locus_tag="VHV99_02655"
     CDS             3149..3529
                     /locus_tag="VHV99_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2917 domain-containing protein"
                     /protein_id="HEX3634386.1"
                     /translation="MDQASPQCMDDDSSLSAYQQDERLPKVVIHFSVAPGPTLTWRVD
                     ADSTLRVQGARLWLTRVNSPYDHWLEPGHEFRLQRGERVWLSTGDERTARVSLSCALP
                     ARHGLVRRWLGRLAWLGLGAPATR"
     gene            complement(3931..4503)
                     /locus_tag="VHV99_02660"
     CDS             complement(3931..4503)
                     /locus_tag="VHV99_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634387.1"
                     /translation="MKAATIGAALMAVAFAAAQPVWAQDALASAAQGVVGAVQPVHVK
                     AEIVGIDPGSRTLTLKGPGGNAVVVLVSQQVAGFEQLKIGDRVDVLYKNALLVKADKV
                     TGKDKGIRARVDTQVYAPASGGFESARQIEVLATVQKIDHKKRLVTLRGAYQTQTLEV
                     GPDVDLKGVKVGDTIHAVFVSAAAVQVTPQ"
     gene            complement(5060..6262)
                     /gene="pcaF"
                     /locus_tag="VHV99_02665"
     CDS             complement(5060..6262)
                     /gene="pcaF"
                     /locus_tag="VHV99_02665"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HEX3634388.1"
                     /translation="MTEAFLCDAIRTPIGRYAGSLASVRADDLGAVPLKALMERNKDV
                     DWNAIDDVIYGCANQAGEDNRNVARMSLLLAGLPQGVPGSTVNRLCGSGMDAVGIAAR
                     AIKSGEAALMVAGGVESMSRAPFVMGKATSAFSRQADIFDTTIGWRFVNPLMKQMYGV
                     DSMPETGENVATDYKISRADQDAFALRSQQKAARAQRDGTLAQEIVGVTIAQKKGDPI
                     TVSQDEHPRETSLETLAKLKGVVRADGTVTAGNASGVNDGAAALLLANEETAKRFGLT
                     PRARVLGIATAGVAPRVMGIGPAPATQKLLARLNMTIDQFDVIELNEAFASQGIAVLR
                     TLGVADDDARVNPNGGAIALGHPLGMSGARLVTTAMYQLHRTQGRFALCTMCIGVGQG
                     IAIAIERV"
     gene            complement(6730..7710)
                     /gene="denD"
                     /locus_tag="VHV99_02670"
     CDS             complement(6730..7710)
                     /gene="denD"
                     /locus_tag="VHV99_02670"
                     /EC_number="1.1.1.410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-erythronate dehydrogenase"
                     /protein_id="HEX3634389.1"
                     /translation="MKVLITGGAGFLGQRLARELLTRGSLKDTDGKQQAITELVLLDV
                     VHGSDFGDSRVRTEVGDIAERSVLERVIDDKTAAIFHLAAIVSGQAEADFDLGMRINL
                     DASRLLLEICRQRGHTPRVLFTSSVAVYGGDLPEIVQNDTALNPQSSYGAQKAIAELL
                     LNDYTRRGFVDGRVVRLPTISVRPGKPNAAASSFASGIIREPLNGETSVCPVASTTRL
                     WLLSPRKAVESLIAGLELDSAALGNQRVLNLPGIAVSVDEMVAALREVAGDEAVKRIV
                     WEPDARVEKIVGSWPGQWDQARAERLGLSGDRSFADVIRAYIADEGIQIR"
     gene            complement(7750..8538)
                     /gene="otnI"
                     /locus_tag="VHV99_02675"
     CDS             complement(7750..8538)
                     /gene="otnI"
                     /locus_tag="VHV99_02675"
                     /EC_number="5.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HEX3634390.1"
                     /translation="MPRFAANLTMMYNEHAFLDRFAAAAKDGFKAVEFLFPYDFRAGE
                     IKARLDANGLTQALFNAPPGDWAGGERGIASLPGREDEFRRSVETALDYTRELGNRKL
                     HVMAGLIGADQSRAAHREVYLKNLAFAAQAAQAENITVVIEPINTRDMPGFFLNRQDD
                     AQAICAEVGAPNLKVQFDCYHCQIVEGDLAVKLKRDMAGIGHIQVAGVPERHEPDIGE
                     VNYPYLFELIDTLGYDGYIGCEYRPRAATSAGLDWVRPYLNAAR"
     gene            complement(8639..9301)
                     /locus_tag="VHV99_02680"
     CDS             complement(8639..9301)
                     /locus_tag="VHV99_02680"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HEX3634391.1"
                     /translation="MTATAALLTTNEARVREEICVTGASLYQRGYTVGSAGNISARLE
                     DGWLITPTDACLGRLDPADIAKVDFNGNAVSGGKPSKTLALHRGIYARNGEARGIVHT
                     HSTHLVALTLAGVWRDDDVLPPITPYYVMKVGHVPLIRYKRPGDPLVAEQIAALAGTV
                     RAVLLERLGPVVWERSVAQASYALEELEETARLWLMTNPQPAPLDDAALDELRTLFGA
                     RW"
     gene            complement(9298..10608)
                     /gene="otnK"
                     /locus_tag="VHV99_02685"
     CDS             complement(9298..10608)
                     /gene="otnK"
                     /locus_tag="VHV99_02685"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HEX3634392.1"
                     /translation="MTTLSRRALLGCIADDFTGATDLANMLVRGGMRTVQTIGVPASN
                     EFVEADALVVALKSRTIPAAEAVAQSLAALKWLRAQGCRQFFFKYCSTFDSTDAGNIG
                     PVTDALLDALSAEGGATAFTIACPAFPENGRTIYRGYLFVGDALLNESGMENHPLTPM
                     RDANLVRVLQRQTGSKVGLVRYDTVAKGVSAVRESFDALRGNGVRMVIADAVSDADLC
                     VLGEACADLTLITGGSGIALGLPDNFRRAGMLAQGNDAAQLPLIEGLSAVLAGSASKA
                     TNAQVAAWRETRPAFRIDPLAAARGAPMVEQALAFAQPYLDKGEPVLIYATATPDEVK
                     AVQRELGVNEAGHLVESTLASIARGLRERGVRKFVVAGGETSGAVVQALEVRTLRIGA
                     QIDPGVPATATTDAEPLALALKSGNFGAIDFFEKALRHFDGAAS"
     gene            complement(10624..11547)
                     /gene="ltnD"
                     /locus_tag="VHV99_02690"
                     /pseudo
     CDS             complement(10624..11547)
                     /gene="ltnD"
                     /locus_tag="VHV99_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006396306.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
     gene            complement(11577..12281)
                     /locus_tag="VHV99_02695"
     CDS             complement(11577..12281)
                     /locus_tag="VHV99_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011354089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEX3634393.1"
                     /translation="MFDKIPARALSDTVAQQLLKQIDKGTFASGGKLPTEAVLAQQFG
                     VSRTVIREAISRLKNEGVVEPRQGSGVFIAAHGAIRPLRIDYAEAVEAGSVLQILALR
                     RAIEAEVASEAAMHRSDADMMSIDAALARIDEVVAEGENGVAEDVAFHRVIAAATGNP
                     YFLKTLTFLNQYLEAGTVVTRRNEALRDDFSRQVRDEHAAIVAAIRAGDPLAARNAAS
                     THMYNAARRLAEAGID"
BASE COUNT         2319 a         4098 c         3744 g         2374 t
ORIGIN      
        1 ctccgctacc gtgtcgcgtt cgttgtctag tgtgatcatg tggtagctgt cggtcaagcg
       61 cacgaatgtc actgaacgac tccccaagtg ctcgcacacc aattccgccg agcggatact
      121 tgttacttcg tcttcctcag aatgcatcac caaggtctcg catgttacac ggctcaaggc
      181 ccgcttcacc gcgaaacgca tccagtcaac ctgctcgatc gccgccaacg gcacgtacgg
      241 ataatggctg ccgcccccct tccgcaaatg gcggcggatc aggttgcgaa tgcgcgcatt
      301 cttgatgcca aacggttcgc cctcaggcac gcgaatcatc cggcgtagcc ccggcacgca
      361 gtacagcaga tagcgcagcg gacgcaggcg tgaaccgccc cagccgtcga ggaataacgg
      421 cggcgacagc agaatcagac gccccgtcgt catgcgctcc cgcgcactca acatcaatgc
      481 aagcaatgcc cccatcgaaa tgcccgcaac atccactctc tcgtaacgcg ccatcaggtc
      541 gcggtacgtg cggctcagca gttgcatgta gtcgctcaat gtcacccggt tcaatgcgtc
      601 agggttcgag ccgtgacccg gcagcgatgg caaatgcacg tcgcaaccga tctgctcgaa
      661 cttgcggtcg agcgcgccaa aatcgtggcg cgtgcctccc aaaccgtgaa tcatcaacac
      721 agcatggcgg gaatgtacgg atacacgagc gaacatcgct aagcctgtcg ttgcacaagc
      781 ttgcgatgct aagcatcccc gactcgcatg actgtcgatt tgataccgcg tttgtggaat
      841 tttgtgcgcc gatatttcga cgcattactc catcgaaacg cagctgcaac tcatccgaac
      901 cgataccgca acttcactgc cgccgctgcg aacaccttca atcccatgcc tgcttgttga
      961 actgataccc ctttccccgc accgtcttga tgcgttgcgg ggtgccggcg tcgtcatgca
     1021 gcttgcgacg cagctttgat atgccgccgt cgatcgtgcg gtcaaggccg tcgaactcga
     1081 tgccgcgcag ttgccgcatc aattcgtctc ggctcactac ctcgcccgcg caactcacca
     1141 acgcccacag caaatcgaat tcggccgatg tcaggtcggg caggctgccg tcggggagtt
     1201 tgaccgtgcg gcttgcgcgg ctgatctcga acctgccgca gatgtagctc tcgggttggg
     1261 cggcctccgg cgtgcgcgcg gtcacgcggc gcagctgcgc cttgatacgc gccagcagca
     1321 cgcgtggctc gacgggcttg tgcacgtagt cgtccgcgcc gaattcgagg cccagtactt
     1381 cgtcgaactg ctcgtcgcgc gcggtcacca tgatgatcac gccgtcgaat tgctcacgcg
     1441 cctcccggca aatctggaaa ccgtccttgc caggcaggtt cacgtcgaga atcaccagat
     1501 ccgggcggcg tttgagaatg gccggcacgg cgccattgcc atgcagcacg gtctcgactt
     1561 cgtattcgtg tttgcgcaga taaccggcga tcagcgcgga caggcggtca tcgtcttcga
     1621 caagcaggat ccggaatggc atggagcgaa tcgttcaggg ctgactgttt taatcgcggc
     1681 gggttcggca tcggccgttc gcaccgcggc tcgcggccga tggatggcgg catgcgtaag
     1741 caacggccgg acgtgcccgc tcgatacaga gcatagcgga tgcgccgcac gtcatgtgac
     1801 acgcgcgaca aaacttgaca aacgcgcaag caattcgacg cggccgcgcg atccgcgccg
     1861 ctagcatcgt cacgattgaa atagcccaag gatccgccgt gacgactgtt tccaacgaaa
     1921 gggctcgcgc ccgcttatcg tggttcgcaa gagccgctgc gtctttcgcg gtcattcagc
     1981 aggccgctca tgcacgctat cccggcaacg gcgcgtttca tcgctggcgg cgcctgcgtc
     2041 tgtggctgcg ctcgctgatg tcgtggcact cgacgcaagt atggctcaaa cgctgcgcca
     2101 tttcgccctt gagcgatctg gtgcaacgtc atcccaccgc gctcgaacgc gtgcatcggc
     2161 cgttcctcca tacgcgcttc agcccgcgcg aacgcttgac agccagcctc gatcatcacg
     2221 ccctgacgca gcagcgcgcg ccgcaactcg ccgcgcgcat cgccgcagtc ggctcggcgc
     2281 cgatcgcgtc cttcggggtg ggcgccgagc gctggtatgt gtcgctcgag tcgatcgaac
     2341 agtttcagaa agaaggcgac tggaccttgt gcatccgcga tgcgcgcgac cagcgcattg
     2401 tctcctgcac cttcagcctc gcctatctgg gcggcaaagt gcgacggcca cgtctttgca
     2461 tcggctccgt gcagggtccg gacagttcgg tcaacggccg cgaactgttt cgcgcgctca
     2521 ccaggcgatg gtacggactg cggccgaaag cgctggtgat ttatctcgcg caatgtatcg
     2581 ccgcggcgct cgatgcgggc ggcaccttca tcgtttccag gcaggctcac atctatgcga
     2641 actggcgcta ttgcctgaga aagcgtcgcg tggccgcgga ctacgatcga ctctcgatag
     2701 aatgcggggc gctcgcgcgc tggaatggct ggttcgtcct cgcaccgccg gcgcgctatc
     2761 tggccgaacg ccagggtgac gacaccggca gcgcaacgcg ccgcaagcgt tacgcattgc
     2821 gaagcgaact cgctgcgcag attcgccgca gcatcgcggc aggcaacaca cagacccagc
     2881 acgcctcgtg ttgtcatttg aatccattgc gattttttat tgacaagata tccggcaaaa
     2941 cggcacactc attcgcatgt tgacgcacac ctccatcgcc ttcgcgatta aaaacccaac
     3001 catgcatgcc atgatcggca ggcatttggg agcatgtgcg cgttagcagt ctgaatctgc
     3061 aaacaagacc caaaggcccc gccggtaacg gaaggggcct tttgttttgt gggcgcctcc
     3121 gtccgccggc tcgatgacgg agagcaagat ggatcaggca agcccgcagt gcatggatga
     3181 cgactcaagt ctgtctgcgt atcaacagga cgagcgtctg ccgaaggtag tcatacattt
     3241 ttccgtagcg ccaggcccaa cgctcacgtg gcgtgtcgat gccgacagca cgcttcgtgt
     3301 gcagggcgcg cgtctatggc tcacgcgcgt caactcgcct tacgatcatt ggctcgaacc
     3361 gggccacgag ttccgtctgc aacgcggcga acgcgtgtgg ctcagcacgg gtgatgagcg
     3421 caccgcgcgt gtgtcgttga gttgtgcgtt gcctgccaga cacggtctcg tcaggcgttg
     3481 gctcgggcgg ctcgcatggt tgggtctggg cgcgccggcg actcggtagc gatgtcccgt
     3541 ggtgatccgg tcaggcggtg gctcaggcgg ctcgcatggt tgggggtggg gctgaggcgc
     3601 ggcaactcgg tagcccgtgt cccatcgcga tccgatgatc gggctttcgt aatgcgcaaa
     3661 agccaacacc cctgcagcga ggggtgttgg ccgatcggtc atgccagctc cgaggagcga
     3721 gggcatgccg cgttgagcgg ctgtgtcgtg ttgcctgaac gcgggccgcc tactggcgtg
     3781 gtgtttactt caccgccatc gcggcagtta cagcggttac ggcgatagcg gcggctgcgg
     3841 agatagcggc ggttgctgcg gttgctgcgg ttgctgcggt tgctgcagtt gctgcagttg
     3901 ccccagttgc ggagatggcg gctcttacag ctattgcggc gtcacctgca cggccgctgc
     3961 cgaaacgaat accgcatgaa tcgtatcgcc gaccttgacc cccttcagat cgacgtccgg
     4021 tccgacttcg agcgtttgcg tctgatacgc accgcgcagc gtgacgaggc gcttcttgtg
     4081 atcgatcttc tgcacggtag cgagcacctc gatctggcgt gcggattcaa agccgcccga
     4141 ggccggcgca tacacctgcg tatcgacgcg cgcacgaatg cccttgtcct tgccggtgac
     4201 cttgtcggcc ttcaccagca acgcattctt gtacagcaca tccacccgat cgccgatctt
     4261 cagttgctcg aagccggcga cttgctggct caccagcacg accaccgcgt taccacccgg
     4321 ccctttgagc gtaagcgtgc ggctgcccgg atcgataccg acgatctccg ccttcacatg
     4381 caccggttgc actgcgccga cgacgccctg cgcggcactg gccaacgcgt cttgcgccca
     4441 caccggttgt gctgcggcga aagccactgc catcaaagct gctccaatcg tcgctgcctt
     4501 catttacgcg ctccttgaga aattgttcga tctggcgtga cggcgatctg aaaccgcagg
     4561 atgccgatta acgccatgag tgcatacatc ttcaggatat caattaccaa aacgagtgtg
     4621 gcgcagcgat tgtacatgcc ttacaagaag ctacgattgt ttatatcgtg atttttaaat
     4681 tacccggcgg cttgatacga aatgccgcaa catggcgcca cagtgccatc ttgcgggttg
     4741 acaacactgc tttaagtctc tcggaaatcc atatatacgg cagcaaagcg taatatgcga
     4801 aaagcattaa acacatactg catggaagtc cgatagcgta ttaactctta tcgccggcat
     4861 tcagcgcgat gcaatcgaat ccgtcattaa caccgattaa aaacaaattc aatagccggt
     4921 gaattcatgc aatgcgcaca aataaaacag ccccgtcgcc tggggcgaag gggcggctca
     4981 tcatatggtc acggccgcgc tatgcgcgaa gcctcaacgc caaagacgct gtggcgctcg
     5041 ctgcgcgagc gccaaaccgt tacacacgct caatagcgat cgcgatgcct tgacccacgc
     5101 cgatgcacat cgtgcacagc gcaaaacggc cttgcgtgcg atgcagttga tacatcgcgg
     5161 tggtcacgag gcgcgcaccg ctcatgccga gcgggtggcc gagcgcaatc gcgccaccgt
     5221 tcggattcac gcgcgcgtcg tcgtccgcca cgccgagcgt gcgcagcacg gcaatacctt
     5281 gcgaggcgaa tgcctcgttc agttcgatca cgtcgaactg gtcgatggtc atgttcaggc
     5341 gagccagcag tttctgcgtg gcgggcgccg ggccgatgcc catcacacgc ggcgcgacac
     5401 ctgccgttgc gatacccagc acgcgagcac gcggcgtaag accgaagcgc ttcgcggttt
     5461 cttcgttggc aagcagcagc gccgctgcgc cgtcgttgac gcctgacgcg ttacctgccg
     5521 tgacggtgcc gtccgcccgc accacgccct tcagtttggc gagtgtttca aggctggttt
     5581 cgcgcgggtg ttcgtcttgc gacaccgtga tcggatcgcc cttcttctgc gcgatcgtca
     5641 cgccgacgat ttcctgcgcg agcgtgccgt cgcgttgcgc gcgtgcggct ttctgctgac
     5701 tgcgcagcgc aaaagcgtcc tgatcggcgc ggctgatctt gtagtccgtt gcgacgtttt
     5761 cgccggtctc cggcatcgaa tcgacgccgt acatctgctt catcaatggg ttgacgaaac
     5821 gccagccaat cgtcgtatcg aagatatcgg cctggcgcga aaacgcgctg gtggccttgc
     5881 ccatcacgaa cggcgcacgg ctcatgcttt cgacaccgcc ggccaccatc aacgcggcct
     5941 cgcccgactt gatcgcgcgc gcggcaatgc cgacggcgtc catgcccgag ccacacagac
     6001 ggttcaccgt cgagcccggc acgccttgcg gcaaaccggc cagcaagagc gacatgcgcg
     6061 caacgttgcg attgtcttca ccggcctggt tcgcgcagcc gtagatcacg tcgtcgatcg
     6121 cgttccagtc gacgtctttg ttgcgctcca tcagcgcttt gagcggcacc gcgcccagat
     6181 cgtcggcacg aaccgacgcc agtgaacccg catagcggcc aatgggggtg cgaatcgcgt
     6241 cgcaaaggaa tgcttcggtc atgtgatgtc tccagttgat gtgctgcgtg cgctacgccg
     6301 aagcggcgaa cgcttcgcga cgccggccgc ttgcgcgtga cgcggctgtg gatgccgtgc
     6361 ggcatcgtcc cggcgggcgc gacaggaacg cctgcatggc aaccgtcgcg atagatcagc
     6421 ttagcgcagg atgatgaacg atgggctccg atttggggcc atttgttcgc taaaagaaca
     6481 caaggtcata tatcgaacaa tcaggctcga taataggcgt gtgcccgaaa ccctgtcaag
     6541 cgaagcccgc cccggatcag ggaatacgtg caggcccgcg gccgttggcg atcgccgaat
     6601 aaaacgtctg aagatcgacg cgctcgtgcc ggcaaagtgc gtttacgccc gctggtacga
     6661 cgctttggag cgttctgctg tccagcatgg ccgttgaaaa acaaaaccgg tacgtcgatg
     6721 ccgccctaat cagcggatct gaatcccctc gtccgcgata taggcgcgaa tcacatcggc
     6781 gaagctgcga tcgccgctca acccgagccg ttcggcgcgc gcttgatccc actgccccgg
     6841 ccagctgccg acaatcttct ctacccgcgc atccggctcc cacacaatgc gcttcacggc
     6901 ctcgtcgccc gccacctcgc gcagcgccgc caccatctcg tcgacactca ccgcaatccc
     6961 cggcagattc aacacgcgct ggttgccgag cgcagcggaa tcgagctcga gtccggcgat
     7021 caggctttcg acggccttgc gcggcgacag caaccacaga cgcgtagtgc tcgcgaccgg
     7081 acacaccgag gtttcgccgt tcagcggctc acgaatgatg ccgctggcaa acgacgacgc
     7141 cgccgcattc ggcttgcccg gccgcacgct aatggtgggc agccgcacca cccgtccgtc
     7201 gacgaaaccc cgccgcgtgt aatcgttgag caacagttcc gcgatcgcct tctgcgcgcc
     7261 gtacgacgat tgcggattca gcgcggtgtc gttctgcacg atctcgggca gatcgccgcc
     7321 atacacagcc accgaactcg tgaacagcac ccgcggtgtg tgcccgcgct gccggcagat
     7381 ttccagcagc aggcgcgacg cgtccagatt gatgcgcatg ccgagatcga aatccgcctc
     7441 ggcctgtccg ctgacgatcg cggcgagatg gaagattgcg gcggtcttgt cgtcgatcac
     7501 acgctcgagc acgctgcgct cggcgatgtc gcccacttcc gtgcgcacgc ggctgtcgcc
     7561 gaaatcgctt ccgtgcacca cgtcgagcag caccagttcg gtaatggctt gttgcttgcc
     7621 gtccgtgtcc ttcaaggaac cacgggtcaa cagttcgcgt gcgagacgct gcccgagaaa
     7681 acccgcgccg ccggtgatca gtactttcat gatgtgcttt gtccctggtt tgacgttgcc
     7741 ctgttctcgt cagcgagcgg cgttcaaata tggcctgacc caatcgagcc ccgccgatgt
     7801 agcggcccgc ggccggtatt cgcaaccgat gtagccgtca taaccaagcg tgtcgatcag
     7861 ctcgaataga tagggatagt tcacttcgcc gatatcgggt tcatggcgct cgggcacgcc
     7921 ggcgacctga atgtggccga tgccggccat gtcgcgtttg agcttcaccg caaggtcacc
     7981 ctcgacgatc tggcagtgat agcagtcgaa ttgcaccttg agattcggcg cgcccacttc
     8041 cgcgcaaatc gcctgtgcat cgtcctgacg attcagaaag aagcccggca tatcgcgtgt
     8101 attgatcggc tcgatcacga cggtgatgtt ttccgcttgc gcggcctgtg cggcaaaggc
     8161 gagattcttc aggtacacct cgcgatgtgc cgcccgcgac tgatccgcac caatcaaccc
     8221 ggccatcaca tgcagtttgc gattgccgag ctcgcgcgtg taatcgagcg cggtttcgac
     8281 gctgcggcga aattcgtctt cgcggcccgg tagcgatgca atgccgcgtt cgcccccggc
     8341 ccagtcgcca ggcggcgcgt tgaagagcgc ctgcgtcaga ccgttcgcgt cgagccgggc
     8401 cttgatttcc ccggcacgga aatcgtaggg aaacagaaac tccactgcct tgaagccgtc
     8461 ttttgccgca gcggcgaaac ggtcgaggaa cgcgtgttcg ttgtacatca tggtcaggtt
     8521 ggcggcgaaa cgaggcatgg cggcaactcc ttgaaaatga ttttggttct gcgtgcggaa
     8581 gcggcgatga aggcatgctc cgctgacaag atttctattt cgcgattggt tggtcgatct
     8641 accaacgcgc gccgaacagc gtccgcagtt cgtcgagcgc cgcatcgtcg agtggcgcgg
     8701 gttgcggatt cgtcatcagc cacaggcgcg ccgtctcttc gagttcctcg agcgcatacg
     8761 acgcctgcgc cactgagcgc tcccacacca cgggaccaag gcgctcaagt aataccgcgc
     8821 ggacggtgcc cgcaagggcg gcgatctgct cggccaccag cggatcgccg ggacgcttgt
     8881 aacgaatcag ggggacgtga cccactttca tcacgtagta cggcgtgatc ggcggtaaaa
     8941 cgtcgtcgtc gcgccatacg ccggcgagcg tcaatgcaac cagatgggtc gaatgcgtgt
     9001 ggacgatgcc gcgcgcctcg ccgttgcgcg catagatgcc ccgatgcagc gccagcgttt
     9061 tcgacggctt gccgccggat accgcattgc cgttgaaatc gaccttggcg atatcggccg
     9121 gatcgagccg gccgaggcac gcatcggtcg gcgtgatcag ccagccgtct tcaaggcgcg
     9181 cgctaatgtt gccggcgctg cccaccgtat agccgcgttg ataaagactc gcacccgtca
     9241 cgcagatttc ctcgcgcacg cgtgcttcgt tcgtcgtgag gagtgcggct gtggcggtca
     9301 tgacgcggct ccatcgaagt gacgcagtgc cttttcgaag aagtcgatcg cgccgaagtt
     9361 gccggacttc aatgcgagcg cgagcggttc ggcgtccgtc gttgccgtgg cgggtacgcc
     9421 cggatcgatc tgcgcgccga tccgcagcgt gcgcacctcg agcgcctgca ccaccgcgcc
     9481 ggaggtctcg ccgcccgcca ccacgaactt gcgcacgccg cgctcgcgca aaccgcgtgc
     9541 gatcgacgcc aacgtggatt cgaccaggtg tcctgcttcg ttgacgccga gttcgcgttg
     9601 caccgctttg acttcgtcgg gcgtggcggt ggcatagatc agcacgggtt cgcctttgtc
     9661 cagatacggc tgcgcaaaag ccagcgcctg ctcgaccatc ggcgcgccgc gcgccgccgc
     9721 aagcggatcg atgcggaacg cgggccgggt ttcgcgccag gccgccactt gcgcgttggt
     9781 cgctttcgaa gcgctgcctg ccagcaccgc cgacaatcct tcaataagcg gcaattgcgc
     9841 ggcgtcattg ccttgcgcga gcatgccggc gcgacggaaa ttgtcaggca ggcccagcgc
     9901 aatgccggaa ccgcctgtga tcaacgtgag atcggcacag gcctccccca gcacgcaaag
     9961 atccgcgtcc gataccgcat cggcgatcac cattcgcacg ccgttgccac ggagtgcatc
    10021 gaacgattcg cgtacggccg acacgccttt cgccaccgtg tcataacgca cgaggccgac
    10081 tttcgatccg gtctgccgct ggagcacgcg tacgaggttc gcatcgcgca tcggcgtgag
    10141 cggatgattt tccatgcccg attcgttcag caacgcatcg ccgacgaaca gatagccgcg
    10201 atagatcgtg cggccgttct cgggaaatgc cgggcaggca atcgtgaaag ccgttgctcc
    10261 accctcagcg gacaatgcgt cgagcaaggc gtccgtgacc ggaccaatgt tgccggcatc
    10321 ggtcgaatcg aaggtcgagc agtatttgaa aaaaaactgg cggcatcctt gcgcacgcaa
    10381 ccacttgagc gcggcgagcg attgcgcgac cgcctcggcg gcgggaatcg tgcgcgattt
    10441 caacgcgacg accaacgcat cggcctcgac gaactcgttc gatgcgggca caccgatcgt
    10501 ctgcaccgtg cgcatgccgc cgcgcaccag catgttggcg agatcggtgg cgccggtgaa
    10561 atcgtcggcg atgcagccga gcaatgcgcg tctggacaaa gtggtcatgg caggctcccg
    10621 cgcttactta gccgccggca aatcgacacc ggggaaaatc ctgatcaccg cggcatcgtc
    10681 ctcgccgccg taccctgcgc tcgatgccat catgaacatc tgatgcgccg ccgccgacaa
    10741 gggcaacggg aatttcgaac ggcgcgcggt atcgagcacg aggcccagat ccttcacaaa
    10801 gatatcgacc gcggagagcg gcgtgtaatc gccgctcaga atatgcggca cgcggttctc
    10861 gaacatccac gaattgcccg cgctatgcgt gatgacctcg tacagcgcgt caggatcgac
    10921 gccttcgcgc aaaccgagcg ccatcgcctc ggccgccacg gcgatatgca cgcccgccag
    10981 caactgattg atgatcttca ccttcgagcc cgcgccgtgc gccgagccca gccgatagac
    11041 cttgccggcc atcgcggcca gcacgtcctc gcaggcggcg taggcggcgt ccgggccgga
    11101 cgtcatcatc gtcatttcgc cggacgcggc gcgcgccgcg ccaccggaca ccggcgcgtc
    11161 gagcatctgc aagccggccg cttcgatgcg ctggccgagg tcgatcgcga agtccggcgc
    11221 cacggtcgcg ctcgcaatca ccacgctgcc tggtttcatc gccgcgagcg cgccttgcgc
    11281 gccgaataac accgtttcgg tttgcgccgc attcacgacc agcgtgacga ccacttcgca
    11341 ttgcgcaccg agctcggcag gtgacgcaca gccgacgccg ccagccgcaa cgaacgcttg
    11401 caggatctcg ctgcgcagat cgcacgcgtg gacctgaaag cccgcgcgca gcagtgagcg
    11461 ggcgacgccc atgcccatcg ctccgagccc gataacaccg acatttctgg acatgctcga
    11521 ctccaggttg gttagccgcc agcgccccga tggaacccgg ctcgcctgtg ttcagttcag
    11581 tcaatacccg cttccgcgag ccgccgtgcc gcgttgtaca tatgggtgct cgccgcattg
    11641 cgcgccgcca gcggatcgcc cgcccggatc gccgcgacga tcgccgcatg ctcgtcccgc
    11701 acctggcgtg aaaagtcatc gcgcagggcc tcgtttcgcc gcgtcaccac ggtgccggct
    11761 tcgaggtact gattcaggaa agtgagcgtc ttgaggaaat acggattgcc ggtggccgcg
    11821 gcaatgacgc ggtggaaagc gacgtcttcg gcgacaccgt tctcgccttc ggccaccact
    11881 tcgtcgattc tggcgagcgc ggcgtcgatc gacatcatgt ccgcgtcgct gcggtgcatg
    11941 gcggcttccg aggcaacttc cgcctcgatc gcccggcgca gcgcgaggat ctgcagtacc
    12001 gagcccgctt cgaccgcctc cgcgtagtcg atgcgcagcg gccggatcgc gccgtgcgcg
    12061 gcgatgaaca cgccgctgcc ctgccgcggc tcgaccacgc cttcgttctt cagccgcgaa
    12121 atcgcctcac ggattaccgt gcggctcacg ccgaactgct gcgccagcac cgcctcggtc
    12181 ggcagcttgc cgccgctcgc gaaggtgcct ttgtcgatct gcttgagcag ctgctgcgcg
    12241 acagtgtcgc tcagcgcgcg ggcaggaatc ttgtcgaaca tggatgcccc aatggatcat
    12301 gttgtcgggt catcatacaa attttgaaac ttgactgcat ccatgcaaac cctgagttct
    12361 ttttagcaaa agcgggcaaa gttggcgaaa cagaacgctt aagctatcgt cacgcctttg
    12421 cgcagcaccg tgccggctga tcgcgcgagc cctgtgcgcc cttcttcgaa cgcatctttc
    12481 aaggtccatg agcaaagccc tgccgccgtc ttccgccgat cctgtcagcg ccgaa
//
LOCUS       DATASH010000088         3352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2187536,
            whole genome shotgun sequence.
ACCESSION   DATASH010000088 DATASH010000000
VERSION     DATASH010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2187536"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..1306
                     /locus_tag="VHV99_02700"
                     /pseudo
     CDS             85..1306
                     /locus_tag="VHV99_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018434479.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
     gene            1508..1738
                     /locus_tag="VHV99_02705"
     CDS             1508..1738
                     /locus_tag="VHV99_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634394.1"
                     /translation="MAIRDLMNGERQHAAFAEAQKLADSGAYHDYTDIEYVLRFDYGL
                     SDVSALLDGQLMHRDLNRRCADAREKLDALSV"
     gene            2012..2463
                     /locus_tag="VHV99_02710"
                     /pseudo
     CDS             2012..2463
                     /locus_tag="VHV99_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090210.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
     gene            2540..>3352
                     /locus_tag="VHV99_02715"
     CDS             2540..>3352
                     /locus_tag="VHV99_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634395.1"
                     /translation="MKVQQINTQPNEPSYAGVAQNDGQGAQSQVSPAKFDTSSTGDTP
                     YRSSADVSSVPDPALTPPAQGAPGSWVEQKSPQMVTRWQRDDQGKPVLKEVNPTQNRD
                     GSTNNIATEFKLDASGHPVLIGAHVDPRPPRDTSQDRTAGDVFVKGVKDGATHPGQFF
                     GALADKATGDESGTNKKRGEEADKVLAQLPVVGTALTITGGLAGERNPDGSPQLPTPD
                     VQPDVPENTFGGHPADGPFRGEPEAKPAAHDGAAGPSNEPQTGKPPAQESGTN"
BASE COUNT          593 a          995 c         1062 g          702 t
ORIGIN      
        1 atttgcagtt cgcggtatcc gcatttgcgt gcgtatttgc acgcggtttc acggcgagcc
       61 gcgccacgcg tcatctacaa ttccatgtcc catgtcgtca aaaggtcggc ccgatgcccg
      121 gctgattttc ctgctcggtg cgctcgccgc gtgcgggccg atttccatcg acatgtatct
      181 gccgagcctg ccgtcgctga cgcaggcctt ttcggtcacc gcgagcgccg cgcaaaccac
      241 gctgacgagc ttcatgttcg gcttttcact cggcatgctg ctgtacgggc cgttgtccga
      301 ttcgatcggt cggcgcccgg tgctgctcgg cggcatcgtg atctatgtga tctcgagcat
      361 cgcgtgcgcc gtgtcgttct cgatcggctc gctggtcgtg tttcgtttcg cgcaggcgct
      421 cggcgcaggc gccgcatcgg tgctctcgcg cgcgattgcc cgcgacgcgc acgggccgac
      481 cgatgccgcg cgtgtgctgt cgatgctcgc catcgtcacg tcgatcgggc cgttgctcgc
      541 gccgctgatc ggcgggcagt tgctgttgct cggcggctgg cgtgtcgtct ttatcgtgct
      601 gacgctattc ggtgcggtgt gcgcggtgac cgcgttcctc aaggtgccgg aaacgtggcc
      661 gcccgaaaag cgtgcgcact cggcgctgct gaaatcattc ggcgcctatg gcaagttgct
      721 gcgcgatccg gtcgcgtggg ggcatatgtt gtgcggcggc atggcgttcg cctcaatgtt
      781 cgcgtacatc accgcgacgc cgttcgtgta catcgaatat ttccatgtat cggcgcagca
      841 ctacggcttt ttgttcgcgc tgaatatcgt cggcatcatg ttcggcaact tcatgaatac
      901 gcgcctcgtc ggccggctcg ggtcgctgcc gatcatctct tttgccgcga ccgtgagtgg
      961 catcgcgtcc ctgttcgtct gtcttgtctc gctgaccggg tggggcgggc tgtggtcgat
     1021 tgtcttcggt ctgttcttcg tggtcggcgt cgtcgggctg ttgtccgcta actgcaccac
     1081 cgatctgatg catcgctatc cagtgaatgc cggcgccgcg gccgcggtgt tcggcgccgt
     1141 gcagttggcg ctcggtgcgt tgtcgagtct cgccgtcgga ctttggcagg atggctcgcc
     1201 caagggcatg ggcgtcgtgg tgggcgttgc gggcgtgctg tgttacgtcg gccggatgct
     1261 ggttgtcaga tggcacggaa tgaaggcgcc ggtcgctgcc gtttgagtct gcgtatcgct
     1321 ggatgggctg ggagagtccg ccgcccaagg cggatcattg ttccctcgcc gaaacgtgcg
     1381 gcaagctcgc ttcggcgtgc cgcgctaaac cgcgcgtcta cggaaaacac caggtgttgc
     1441 gcgcgcgcga ccgtgtgtgt caatttgcat tgctatgatg gcgtcactgt tcctcggagc
     1501 ggcaatcatg gcgatcagag atttgatgaa cggcgagcgg caacatgctg ccttcgcgga
     1561 agcgcagaag ctggctgaca gcggcgccta tcacgattac acagacatcg aatacgtctt
     1621 gcgctttgac tacggtttgt ctgatgtatc ggcgctgctc gacggccagc tgatgcatcg
     1681 cgacctcaat cgccgctgcg ccgatgcgcg tgagaagctg gacgcgctta gcgtgtgagg
     1741 gaatgggcgt atgggcgtta gtgacgggcc tattgcgatt ggcgcgtggc tgaaccggtc
     1801 gagcgctcgc ttgcgaatcg cgtgaggcgc gtgaagatct gagacgactg aggcgctgca
     1861 cggagcgttc gcgacggttg cttcttgcgc gatgtcttca ttgttatcgc caaagatctg
     1921 cacgcgagcg aactgcgtgc gagtgagccg cgtgcagtgt catgcgcata atccgctccg
     1981 cgaaagcacc atcccctttg gcgttgcatt catggctcat agccagcacg ctccgcctgt
     2041 gccgtacacg gacaatcccg cggcactggc aaaacctggt gggcattaca gccacgtcgc
     2101 gatcgcgaat ggttttgtgt tcgtctcagg gcagttgccg atcaacgcac agggcgaaaa
     2161 tcgtcgctga tgccgtcgtt cgaagttcag accgagcagg tgttggccaa tcttcgggca
     2221 gcgctcgaag gcgccggcag cggcattgcg caactcgtgc aagggcacgt caatatcgcc
     2281 gatgtcgaac actgggcgag cttcaatcag atccacgcga gttggccggg cgacgcccgg
     2341 ccggcgcggg ttgtcgcgcc ctcccgcggc cgccatatta accgccgacg tcaacagtca
     2401 cgattgagcg tcgtgagctt gcatcgatat aagcaccacc ttgtcacaga gcgttactgt
     2461 tgaaatggac tgacgcctcc tactcggagc cgtgttctgc gaagccgaat ttgacctttc
     2521 acatagatag gaatcgcgaa tgaaagtcca gcaaatcaac acgcaaccga acgaacctag
     2581 ttacgccggc gttgcacaaa acgacgggca gggtgcgcag tcacaggtat cgcccgcgaa
     2641 attcgataca tcaagtaccg gtgacacacc gtaccgcagt tcagccgatg tgagttccgt
     2701 gccggacccc gcgctaactc cccctgccca aggtgcgccc ggcagctggg ttgagcagaa
     2761 atcgccccag atggtgacgc gctggcagcg ggacgaccaa ggcaaaccag tccttaaaga
     2821 agtcaatccg acacagaatc gcgacggctc aacaaacaac attgcgactg agttcaagct
     2881 cgacgccagc gggcaccccg tccttattgg tgctcatgtt gacccgcgtc ctccgcgcga
     2941 caccagccag gaccggacag cgggagatgt cttcgtgaaa ggcgtcaagg atggagccac
     3001 tcaccctgga caattcttcg gcgcgcttgc agacaaggcc acgggggatg aatcgggcac
     3061 gaataaaaag aggggcgagg aagcggacaa ggtacttgcc cagttacctg tcgtcggtac
     3121 ggcgctaacc ataacgggtg ggcttgccgg agaacgcaat ccggatggga gcccacaact
     3181 tccgaccccg gacgttcagc cggatgtgcc ggaaaatacg ttcgggggac atccggcaga
     3241 tgggcctttt cggggcgaac ctgaagcgaa acccgcggcc catgatggtg cggccggtcc
     3301 gtcaaatgag ccccaaacgg gtaagcctcc tgctcaagaa tcgggaacga at
//
LOCUS       DATASH010000089         7798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1396158,
            whole genome shotgun sequence.
ACCESSION   DATASH010000089 DATASH010000000
VERSION     DATASH010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7798
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1396158"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..989)
                     /locus_tag="VHV99_02720"
     CDS             complement(72..989)
                     /locus_tag="VHV99_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX3634396.1"
                     /translation="MADIQNTVPTAVTPPSGRAIAAREFWANFSRNRGAVCAGIVVLA
                     LIFIAIFAPLIAPHSPIEQYRDFVKIPPAWLDGGNWKFILGTDEAGRDILSRLMYGAR
                     LSFWIGFVSVVLALIPGIVLGLIAAFFEKWADTPIMRIMDVLLALPSLLLAVAVVAII
                     GPGLVNTMLAIAIVALPGYVRLTRASAQGELQKEYVTASRVAGASTLRLMFSQVLPNC
                     TAPLIVQATLGFSSAILDAAALGFLGLGVQPPSAEWGAMLASARDYIDSAWWIVTMPG
                     LSILISVLAINLLGDGLRDALDPKLKRMA"
     gene            complement(997..2007)
                     /locus_tag="VHV99_02725"
     CDS             complement(997..2007)
                     /locus_tag="VHV99_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX3634397.1"
                     /translation="MFRFVLRRIGMVIPTFIGITILAFALIHLIPGDPIEVMMGERGV
                     DPAMHAAAMHRLGLDESLPMQYVHYIGRAMHGDLGTSIITNTSVMGEFLARFPATVEL
                     SICAMFFALIVGLPAGVFAALRRGTVVDHGVMGTALTGYSMPIFWWGLILIMVFSVKL
                     GWTPVSGRIAVEYDIPHVTGFMLIDALMSTDEGAFRSALSHLILPAIVLGTIPLAVVA
                     RMTRSSMLEVLREDYIRTARAKGLSPARVIVVHALRNALIPVVTVIGLQVGTLLAGAV
                     LTETLFSWPGIGKWLIDAIGRRDYPVVQGGILMIATLVIVVNLVVDLLYGVLNPRIRH
                     TR"
     gene            complement(2147..3775)
                     /locus_tag="VHV99_02730"
     CDS             complement(2147..3775)
                     /locus_tag="VHV99_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006417151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3634398.1"
                     /translation="MKQNNLLRAARVTTLVAAAAASMLGATSAHAQIPNKTLVYCSEG
                     SPAGFDPAQYTTGVDFTANTFTVYNRLVEFERGGTKVEPGLAEKWDVSPDGKTYTFHL
                     RHGVKFQTTSFFKPTREFNADDVVFTFQRMLDTNQPFRKAYPVQFPYFTDMGLDKLIT
                     SVEKVDPYTVKFTLKEVNAPFIQNLAMEYASILSGEYADQLLKAGKAADINQYPVGTG
                     PFIFRSYTKDATIRFDGNPDYWKPNAVKISKLIFSITPDAGVRVQKIKRDECQVMSYP
                     RPADIAPLKAEANIDMPSQPGFNLGYLAYNVTHKPVDKVEVRQALDMAINKKAIVESV
                     YQGAGQAATNPMPPTQWSYDKNLKSASYDTDKAKALLAKAGYPNGFDITLWAMPVQRA
                     YNPNARLMAEMIQADWAKIGVKAKIVTYEWGEYIKRAHAGEDDTMLIGWTGDNGDPDN
                     WLGTLLGCEAVNGNNFSKWCYKPFDDLIQKGRVTSDQAARTTAYTQAQQIFAQQLPFS
                     PIAHSTVYQPVSKKVVDMRIEPLGYARFDGVSVK"
     gene            complement(3980..5623)
                     /locus_tag="VHV99_02735"
     CDS             complement(3980..5623)
                     /locus_tag="VHV99_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3634399.1"
                     /translation="MTVSRLLFAVRHTCLPILSAYALLAAGLAPAGAGAATLPDKTLV
                     FCSEGSPAGFDSAQYTTSVEFTAASYTVYNRLVEFARGSTDIEPGLAEKWDVSQDGLQ
                     YTFHLRHGVKFQTTSFFKPTREFNADDVVFTYQRMLDPEQPFRKAYSVPFPYFTDLGL
                     AKNIAKVEKLDPYTVRFTLKEVDAPFLQQIAMPFASILSAEYTDQLLKAGKASDINQY
                     PVGTGPFIFRSYTKDDTIRFDGNPDYWKPGIVKVGKLIFAITTDPAVRLQKLKRGECQ
                     VMSYPRPADIPQIKADSSLALPSEVGFNLGILGYNTTKKPLDNVLVRRALDMSINKKA
                     IIESVYQGAGQIATNPMPPTQWGYDKNLKDAPYDIDKAKALLKEAGYPDGFDLTLWAM
                     PVQRPYNPNARLMAEMLQSDWAKIGVKVTIATYEWGEYIRRAHAGEHEAILIGWTGDY
                     GDPDNWLGVLLGCDAVKGSNFSKWCYKPFDDLIRKGRGTTDLGERTKAYMEAQEIFKQ
                     QVPFTPIAHSTVYQPISKDVTGFKIDPFGPTQFMSVGLK"
     gene            6127..7062
                     /locus_tag="VHV99_02740"
     CDS             6127..7062
                     /locus_tag="VHV99_02740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobile mystery protein B"
                     /protein_id="HEX3634400.1"
                     /translation="MRQLRLEQVQASVAAYSDLTNRRPPPRGWLKAIRESLGLTERQQ
                     ADRLGITGSTLHKSELAEAEERITLGQLRKLAEGLDCELVYALVPRKPLTQVVEDRAR
                     SIALQEVSGVAHTISLDEQDGQTPLDPDELAGLIPTHLTTKGDLNDWEQENILQAVQW
                     ARRQRKVDVLSEEFCRSLHKKMFDQTWSWAGAFRKSDKNIGCDWTQVSVKLRNLFDNT
                     RWWVDNDTFPPDEIAARFHRELVWIHPFPNGNGRHSRMMADALLRSMKQEPFSWGGGG
                     TLVKADNARSDYLTALRAADKGNYSLLLVFVRSTA"
     gene            complement(7363..>7798)
                     /locus_tag="VHV99_02745"
     CDS             complement(7363..>7798)
                     /locus_tag="VHV99_02745"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEX3634401.1"
                     /translation="VAGYPEYHPQSRSPRHDLENFAHKVKAGANSAITQYFFNADAYF
                     RFVDDARKLGVEVPIVPGIMPITNFSQLMRFSEMCGAEVPRWVARRLESFGDDRESIR
                     AFGLDVVTDLCGRLIDAKVPGLHFYTLNGAAATKAICERLGA"
BASE COUNT         1614 a         2307 c         2330 g         1547 t
ORIGIN      
        1 cacgcgcgct gccgcccgca gcggactgct cgggagccag cgaatgattt gaacgtggag
       61 tttgttccat gtcatgccat ccgtttcagt ttgggatcga gtgcgtcgcg cagcccgtcg
      121 ccgagcagat tgatcgcaag caccgagatc aggatggaga gccccggcat cgtgacgatc
      181 caccaggcac tgtcgatata gtcgcgcgcc gaggccagca ttgcgcccca ctcggccgac
      241 ggcggttgca cgcccaagcc gagaaagccg agcgcggcgg catcgagaat cgccgacgaa
      301 aaacccaggg ttgcctgcac gatcagcggt gcggtgcagt tcggcagcac ttgcgagaac
      361 atcaaacgca gcgtgctcgc gcctgcgacg cgcgaagccg tcacatactc tttctgcaat
      421 tcgccttgtg ccgaagcacg cgtcaaacgc acatagcctg gcaatgcgac gatggcaatc
      481 gcgagcatcg tattgacgag acccggaccg atgatcgcga ccaccgccac ggccagcagc
      541 agcgagggca acgcgagcag cacgtccatg atgcgcatga tcggcgtgtc ggcccacttc
      601 tcgaagaatg ccgcgatcaa gccgagcacg atgcccggaa tcagcgcgag caccaccgag
      661 acgaagccga tccagaacga caggcgcgcg ccgtacatca gacgcgagag gatgtcgcgg
      721 cccgcttcgt cggtgccgag aatgaacttc cagttaccgc cgtcgagcca ggcggggggg
      781 atcttcacga agtcgcgata ctgctcgatc gggctatgcg gcgcgatcaa cggcgcgaag
      841 atcgcgatga agatcaacgc cagcacgacg atgccggcgc aaaccgcacc acggttgcgt
      901 gagaagttcg cccagaattc acgggcggcg atggcgcggc cgctgggcgg cgtcaccgct
      961 gtagggacgg tattttgaat gtctgccatt ttcagcttac ctcgtatggc gaatgcgcgg
     1021 attcagcacg ccgtacaaca gatcgacgac gaggttcacg acgatgacca gcgtagcgat
     1081 catcaggata ccgccctgca ccaccggata gtcgcgccgg ccgatcgcgt cgatcagcca
     1141 cttgccgata cccggccacg aaaacagcgt ctcggtcagc accgcgccgg cgagcaaggt
     1201 gccgacctgc aagccgatca cagtcaccac cggaatcagt gcattacgca aggcatgcac
     1261 aacgatcacc cgcgcaggcg acaagccctt cgcgcgtgct gtgcgaatgt aatcctcgcg
     1321 caatacttcg agcatcgacg aacgcgtcat gcgcgcgacc acggcaagcg gaatcgtgcc
     1381 gagcacgatg gccggcaaaa tcagatggct cagcgcggat ctgaacgcgc cttcatcggt
     1441 ggacatcagc gcgtcgatca acatgaaacc cgtgacgtgc ggaatgtcgt attcgaccgc
     1501 gatacggccc gacaccggcg tccagccgag cttcaccgag aacaccatga tgaggatcaa
     1561 gccccaccag aagatcggca tcgagtagcc ggtcagcgcc gtgcccatca cgccatgatc
     1621 gaccaccgtg ccgcgcctca aagccgcgaa cacgcccgcc ggcaagccga cgatcagcgc
     1681 gaagaacatc gcgcagatgg agagttcaac ggtggcggga aagcgtgcga ggaattcgcc
     1741 catcacgctg gtgttggtga tgatcgaggt gccgaggtca ccatgcatcg cgcggccgat
     1801 gtagtggacg tattgcatcg gcaaggactc gtcgagcccg aggcggtgca tcgcggcggc
     1861 atgcatggcc ggatcgacgc cgcgctcgcc catcatcact tcgatggggt cgcccggtat
     1921 caggtgaatc agcgcgaacg caaggatcgt gatgccgatg aaggtcggta tcaccatgcc
     1981 aatacggcgc aaaacaaagc ggaacatggt tcgtccctat ggtcttgatg aagaaaaaac
     2041 gcaaccggcg acgagggctt gtgaccccgg tcgccggacc atttcgacca gttttctaac
     2101 cgtgcgaaaa gctactgcgt aagtttgact gcctctactc tatgacttac ttgacgctga
     2161 cgccgtcgaa gcgcgcatag ccgagcggct cgatacgcat gtcgaccacc ttcttgctga
     2221 caggttggta caccgtcgag tgggcgatcg gcgagaacgg cagctgctgc gcgaaaatct
     2281 gctgcgcttg cgtgtatgcc gtcgtgcgcg cggcttgatc ggacgtgacg cgacccttct
     2341 ggatcagatc gtcgaacggc ttgtagcacc acttcgagaa gttgttgccg ttcaccgctt
     2401 cgcaaccgag caaagtgccg agccagttgt ccggatcgcc gttgtcgccg gtccagccga
     2461 tcagcatcgt gtcgtcttca cctgcgtgag cgcgcttgat gtactcgccc cattcatacg
     2521 tgacgatctt cgccttcacg ccgatcttgg cccagtcggc ctggatcatt tccgccatca
     2581 gacgggcatt cgggttgtag gcgcgttgca ccggcatcgc ccatagcgtg atgtcgaagc
     2641 cgttcggata accggccttg gccagcagcg ccttggcctt gtccgtatcg tacgatgcgc
     2701 tcttcaggtt cttgtcgtac gaccattggg tcggcggcat cggattcgtc gcggcctggc
     2761 ctgcgccttg atacaccgac tcgacgatcg ccttcttgtt gatggccatg tcgagcgcct
     2821 gacgcacttc gaccttgtcg accggtttgt gcgtgacgtt gtacgccagg taaccgaggt
     2881 tgaagcccgg ctgcgacggc atgtcgatgt tcgcttctgc cttcaacggc gcgatgtcgg
     2941 ccggacgcgg atagctcatc acctggcatt cgtcgcgctt gatcttctgc acgcgcacac
     3001 cggcgtccgg cgtgatcgag aagatcagct tcgagatctt caccgcgttc ggcttccagt
     3061 agtccggatt gccgtcgaag cggatcgtcg cgtctttcgt atagctgcgg aagatgaacg
     3121 ggcccgtacc caccgggtac tggttgatgt cggcggcctt gccggccttc agcagctggt
     3181 ccgcatattc acccgagaga atcgacgcat attccatcgc caggttctgg atgaacggtg
     3241 cgttgacttc cttcagcgtg aatttgaccg tgtacgggtc gaccttttcg acgctggtaa
     3301 tcagcttgtc gagccccatg tccgtgaagt acgggaattg gaccgggtac gccttgcgga
     3361 acggctggtt cgtgtccagc atgcgctgga acgtgaacac gacgtcgtcc gcgttgaatt
     3421 cgcgcgtcgg cttgaagaac gacgtggtct ggaacttcac accgtggcgc agatggaacg
     3481 tgtaggtctt gccgtccggc gagacatccc acttttctgc caggcccggc tcgactttcg
     3541 tgccgccgcg ttcgaattcg acgaggcggt tataaacggt gaacgtgttg gccgtgaagt
     3601 cgacaccagt cgtgtactgg gccggatcga aacccgcagg gctgccttct gagcagtaaa
     3661 cgagtgtttt gttcggaatc tgtgcatgcg cgctcgtagc acccagcatc gatgccgctg
     3721 cagctgcgac gagcgtggta acacgcgcgg cgcgcaacag gttgttttgc ttcatgtttc
     3781 ctccaggttc ggggctggct tccgccagcg tagcgcgata ttacttgagc ttggctagca
     3841 ccaacaagcg gaggaaaata ctcccgttga cgatcggaaa cagatgaggc ataagggcgg
     3901 ccgcacgccg gcgcgcgccg gaacgtgcgg cactagaagc agaggccgca aaaagcggcc
     3961 cgcgagtgct gactgacgac tatttcaagc cgacgctcat gaattgtgtc ggaccgaacg
     4021 ggtcgatctt gaagccggtg acgtctttgc tgatcggttg atacacggtg gagtgggcaa
     4081 tcggcgtaaa aggaacctgc tgcttgaaga tttcctgcgc ctccatatag gccttggttc
     4141 gctcgccgag atcggtggta ccacggccct tcctgatcag gtcgtcgaaa ggtttgtagc
     4201 accattttga aaaattgctg cccttcaccg catcgcagcc gagcaacacc ccaagccaat
     4261 tgtcaggatc gccgtagtct ccggtccagc cgatcaggat cgcctcgtgt tcgccggcat
     4321 gggcgcggcg gatgtactcg ccccactcgt acgtggcgat cgtcaccttg acgccgatct
     4381 tcgcccaatc ggactgcagc atttcggcca tcagacgggc gttcgggttg tagggccgct
     4441 gtaccggcat ggcccacagg gtcaggtcaa agccgtccgg gtagccggcc tccttcagta
     4501 acgccttggc tttgtcaatg tcgtaaggcg catctttcag gttcttgtca tagccccatt
     4561 gggtcggcgg catcggattc gtcgcgattt gcccggcgcc ctgatacacc gactcgatga
     4621 tcgctttctt gttgatcgac atatccagcg cgcgtcgcac caacacgttg tcgagcggct
     4681 ttttcgtcgt gttgtacccg aggatgccca gattgaagcc gacctcgctc ggcaaagcca
     4741 gcgacgagtc ggccttgatc tgtggaatat ccgcgggacg cgggtagctc atcacctgac
     4801 attccccacg tttcagtttc tgcagccgca ccgccggatc cgtggtaatc gcgaagatga
     4861 gcttgccgac cttcacgatg cccggcttcc agtaatcagg attgccgtcg aagcggatcg
     4921 tgtcgtcctt cgtatagctg cggaaaatga acggcccggt accgaccgga tattggttga
     4981 tatcggaagc cttgccggct ttcagcaact gatccgtgta ttccgccgac agaatcgacg
     5041 caaacggcat cgcgatctgc tgcaggaacg gcgcatcgac ctcttttagc gtgaaacgca
     5101 ccgtatacgg atccagcttc tcgactttgg cgatgttctt cgcaaggcca agatcggtga
     5161 aatacgggaa cggcaccgag tacgccttgc ggaacggctg ttccggatcg agcatccgct
     5221 gataggtgaa aacgacgtcg tcggcgttga actcgcgggt cggcttgaaa aaggaggtgg
     5281 tctggaattt cacgccatgg cgtagatgga acgtgtactg cagaccgtct tgcgacacat
     5341 cccacttctc ggcgaggccc ggctcgatat cggtgctgcc gcgcgcgaac tccacgagcc
     5401 ggttgtagac cgtgtacgag gcggcggtga attcgacgct ggtggtgtac tgtgcactgt
     5461 cgaagcccgc cgggctgcct tcggagcaga acacgagggt tttgtcaggc aaggtggccg
     5521 cgccggcacc ggcgggcgcg aggccggcag cgaggagcgc gtaggcgctc aggattggca
     5581 ggcaggtatg gcgaactgca aacagcaatc gggatactgt catcacgttc tccgcacagc
     5641 agccacgtca gctgcgcttc gatcatagac agcacaaaaa tcgggttcaa tgcgggctta
     5701 cgcgccgcaa ggaaagattt tcacgacgat tgccgcgccg cactaagaat tttcggcgga
     5761 aatacagagc tacgcctagg tgctacctgc gcaaagcgtc aatgctcggg gtttccttta
     5821 tcgaaccggg cgaccgacgt ttgaagcaaa ctgtctcgca attgctggct tgtggtgccg
     5881 caatgagaga cttttgcttc cgtaaaatga agcaattata tgaattctga tgccctatgg
     5941 ggaagtattc atatattttt tgctttccca tgatgaagca agcaattcga ttctgtgtca
     6001 ttacaccgac gctgcatata ttaatttgct tcatcttggg gaagcgaaga attgactttt
     6061 gcttcactat aggctatcgt ttgtcgcgtt cttgatcgcc tataggccag caaaatggat
     6121 tccagaatga gacagctccg gcttgagcag gtccaggcgt cagtcgcagc gtactcggat
     6181 ctgaccaacc gccggccccc gcctcgtggg tggctgaagg ccatccgaga gtcgctgggg
     6241 ctcaccgaga gacagcaagc cgacagatta ggcatcaccg gttcgaccct gcacaagtcg
     6301 gagttggcgg aagctgagga gcgaatcacg ctcggacaac tgcgcaagtt ggccgaaggg
     6361 ctggactgcg agctggtcta tgctctggtg cccagaaagc cactgactca agtggtcgaa
     6421 gaccgggcgc gctcaatcgc cctgcaggaa gtgagtggag ttgcgcacac catcagtttg
     6481 gacgaacagg acggccagac accgctcgac cctgacgaac ttgcagggct cataccaacg
     6541 cacctgacca ccaagggcga cctgaatgac tgggagcagg agaacatcct gcaagccgtg
     6601 caatgggctc gccgtcaacg caaggtcgat gtcctcagtg aagagttctg tcgcagcctt
     6661 cacaagaaaa tgttcgacca gacctggtcg tgggcaggcg cgtttcgcaa gtccgacaag
     6721 aacatcggat gtgactggac gcaggtcagc gtcaaactga gaaacctttt cgataacacg
     6781 cggtggtggg tcgacaacga cacattcccg cccgatgaga ttgccgcgcg atttcaccgc
     6841 gagctggtgt ggattcatcc ctttccaaac ggcaatggtc gacactcgcg aatgatggct
     6901 gatgcgctct tgcgcagcat gaaacaagag ccgttttcgt ggggcggcgg cgggacattg
     6961 gttaaagcgg ataacgcccg gtcggattac ctaaccgcct tacgtgcggc tgataaaggt
     7021 aactacagct tgctcctggt cttcgtgcga agtactgctt agccgtcgca cggacaagaa
     7081 ggacgccaag tcatttttga cgggccaaag agctttgcgc tgttgcgcgt gcagccctgc
     7141 atgaccgttc tttcgtcgag gcccggcagc tccgcaatcg cgagcgcgca gcactcgaca
     7201 ccacttggcc aaggctcgtg tgggctttcg atgcccataa aaagcgagcc tccgaagagg
     7261 ctcgcggtaa tcaccatgcc tgcccgttta ccaacacctt tgtggtacgg gtagctaaat
     7321 aggccgcgcg gttttgtaca gcgtccattc agacttcaga cctcaagcgc ccagccgttc
     7381 gcagatcgcc ttggtcgccg ccgcgccatt gagcgtgtag aaatgcaaac ccggcacctt
     7441 cgcgtcgatg agacgcccgc acaggtccgt caccacatcc agcccaaacg cgcgaatcga
     7501 ctcgcggtcg tcgccgaagc tttccagacg gcgcgcgacc cagcgaggca cttccgcacc
     7561 gcacatctcc gagaaacgca tcagttgaga gaagttcgtg atcggcatga tgcccggcac
     7621 gatcggcacc tcgactccga gcttcctcgc gtcgtcgacg aaacggaagt acgcgtcggc
     7681 gttgaagaag tactgcgtga ttgcggaatt ggcgccggcc ttcaccttat gagcgaagtt
     7741 ttccagatcg tgacgcggcg agcgcgactg cgggtggtac tccggatagc cggccacc
//
LOCUS       DATASH010000090         5679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6736797,
            whole genome shotgun sequence.
ACCESSION   DATASH010000090 DATASH010000000
VERSION     DATASH010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5679
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6736797"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1156)
                     /locus_tag="VHV99_02750"
     CDS             complement(<1..1156)
                     /locus_tag="VHV99_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX3634402.1"
                     /translation="MQSTQRPLPSADALFEHAACGLLLAASDGRILRVNATFCGWLGY
                     EAAALAPTKHIQDLLTAGGKVFYQTHWSPLLQMQGSVAEVKLNMMHRDGRMVPMLLNA
                     VLRRHGDSSYLEVAALIVADRHKYEQELLLARRNAEASVAAHKLAQKELQESRDVLSL
                     AMRGARMGAWSLEPKSGKFWWSRELEALAGYAEGRFANMPRGFFELIHPGDLIALNEA
                     VDHAVASSDDYVAEFRFLHASGDWCWMEGRGRATYDRHGEPMTIYGLGTDITERKDAQ
                     TVLLRQAAIFEHLSDAIVITDLRGNITDVNAGAERMLGYRMREILGKPIAIFHPPEDA
                     LRIRREALAALAADGTWRGELTFVRRDGSRGVCETVVKPLANARGDIYGAL"
     gene            complement(1161..1964)
                     /locus_tag="VHV99_02755"
     CDS             complement(1161..1964)
                     /locus_tag="VHV99_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3634403.1"
                     /translation="MSISQRNNVKISGSGERTMVLAHGFGCDQTMWRYLVPAFHGEYR
                     TVLFDLVGSGASDLSAYDIGKYDSLQGYADDLIEIVKEVADAPVVFVCHSVSAMIGLL
                     ASLKAPELFAAQIMVGPSPCYVNDGDYIGGFTREDIEDLLRTLESNYLGWSSTMAPAI
                     MGAPAQPELGVELTNSFCRTDPEIARHFARVTFLSDHRAILPRATTPTLILQCSDDII
                     APRAVGEYMQRMMPASTLHIIENVGHCPHLSSPGASAAAMCAFLEPLRR"
     gene            complement(2087..2989)
                     /locus_tag="VHV99_02760"
     CDS             complement(2087..2989)
                     /locus_tag="VHV99_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX3634404.1"
                     /translation="MQSIIDEHALYVAKHPGTFILQTLKAFRANQGLLLAGAVAYYAL
                     LSIVPLMILIVIVLSRLVPQHVLLAALAHLLQWLVPGQSTALVHELANFLAHRAVIGW
                     VLLLTMIFFSSLAFTVLENAMSLIFVHRVVVRRRHFLLSALLPYCYILFLGVGLLIVT
                     FVSSGLEAVGAEGIDLFGLHVSLQGLSRVVLYLLGLAGEIFVLTSIYMVMPVGQPSLK
                     HALFGGIVAAVLWEITRHVLVWYFATLSQVSVVYGSLTMAIVILFSLEWLATLLLFGA
                     QVISQYERFGLEPAEAKPQEIKTA"
     gene            3165..4211
                     /locus_tag="VHV99_02765"
     CDS             3165..4211
                     /locus_tag="VHV99_02765"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     exonuclease"
                     /protein_id="HEX3634405.1"
                     /translation="MNDATDQAELADLVVARPEGLYCPPGDFYIDPWQPVERAVITHA
                     HSDHARFGHRHYLASRAGANVLLSRLPGITLQTLAYGERLEIGGAGVSLHPAGHVLGS
                     SQLRIEHKGRVWVASGDYKLDPDPTCDAFEPVRCETFITESTFGLPIYRWDAPQTIFD
                     GIDSWWRHNAAEGRASVLFCYSFGKAQRVLASVDAGIGPLFCQGAVEPLKRAYRDAGV
                     RLPAVRPVSEIQVKDKAAFRHALIVAPPSAQGSAWLKRFGDYSDAFASGWMRLRGARR
                     RRGVDRGFVLSDHADWPSLQSAIQATGAERVIVTHGSVEPMVRWLREQGLEAGAFATQ
                     YGDDTVEADAAGHS"
     gene            4208..>5679
                     /locus_tag="VHV99_02770"
     CDS             4208..>5679
                     /locus_tag="VHV99_02770"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX3634406.1"
                     /translation="MKRFAALYTALDATTSTHDKLDALTSYFAAAAPEDAAWASYFLA
                     GGKPRQSVPTRLLREIARERAGLPEWLFEESYHAVGDLAETIAHILPPAQRSTELGLT
                     QWIEQRVLTLRGVAPEELRVRLLSYWDELDWGGRFLLTKLIGGGFRVGVARQLVVRAL
                     ASVAGVDHKRIAQRMVGWTDSQLKPDAARYLRLIAPEPGGDATHSEPTHDSDLGLPYP
                     FFLAHPLQADPTTLGEPSNWQIEWKWDGIRAQLVKRGGRVWLWSRGEDLITERFPEVA
                     ALGEALPDGFVIDGEILAWEPGAASPLPFARLQPRIARKTLTKKILADSPATLLAYDL
                     LEALGKDLRMTPLAERRAQLDGLATSLDGTFAGDLLRVSPVISASDWAALAKLRDESR
                     ARGVEGLMVKQRESMYGVGRTKASGTWWKWKIDPYAVDAVLVYAQRGHGRRASLYTDF
                     TFAVWDEANGVRTLVPFAKAYSGLTDEEMRQVDAIVRKTT"
BASE COUNT         1066 a         1789 c         1828 g          996 t
ORIGIN      
        1 tgagcgcgcc gtagatgtcg ccgcgtgcgt tcgccaacgg tttgaccacc gtttcacaga
       61 ccccgcggct gccgtcgcgc cgcacgaaag tcagttcgcc acgccacgtg ccgtcggccg
      121 cgagggcggc gagcgcttcc cggcggatgc gcaacgcgtc ttccggcgga tggaagatcg
      181 cgatcggctt gccgagtatt tcgcgcatgc gataacccag catgcgctcg gcgccggcgt
      241 tgacatcggt gatgttgccg cgcaggtcgg tgatgacgat cgcgtcgctc aggtgctcga
      301 aaatcgccgc ctggcgcagc agcaccgtct gcgcatcctt gcgttcggtg atgtcggtgc
      361 cgaggccgta gatcgtcatt ggctcgccgt gacggtcgta ggtggcgcgg ccgcggcctt
      421 ccatccagca ccagtcgccg cttgcatgca gaaagcggaa ctcggccacg taatcgtcac
      481 tgctggccac ggcatggtcg accgcctcgt tgagcgcgat caggtcgccg ggatggatca
      541 gttcgaaaaa gccacggggc atgttcgcga agcgtccctc ggcgtaaccc gcgagcgctt
      601 ccagttcgcg gctccaccag aacttgcccg acttcggttc gagcgaccac gcccccatgc
      661 gcgcgccgcg catggcgagg ctcagcacgt cacggctttc ctgcaactct ttctgcgcga
      721 gtttgtgcgc ggcgaccgac gcttccgcgt tgcggcgcgc gagcagcaac tcctgctcgt
      781 acttgtgacg atccgcgacg atcagcgccg ccacttcgag gtagctgctg tcgccatgac
      841 ggcgcagcac cgcgttgagc agcatcggca ccatgcggcc gtcccgatgc atcatgttca
      901 gtttgacctc ggccaccgaa ccctgcattt gcagcagcgg cgaccaatgc gtctgataga
      961 acaccttgcc gccggcggtc agcagatcct gaatatgttt cgtcggggcg agcgccgccg
     1021 cctcatagcc gagccagccg caaaaggttg cgttcacgcg caggatccgg ccgtcggagg
     1081 cggccagcag caggccgcag gccgcgtgct cgaataaggc atcggcggac ggcaatggac
     1141 gctgcgtgct ttgcatcgtc ttaccgtctc agcggctcga gaaaggcgca catggccgcg
     1201 gcgctcgcgc caggcgagct cagatgcggg caatggccga cgttctcgat gatgtgcagc
     1261 gtgctggcgg gcatcatccg ttgcatgtat tcgccgacgg cgcgtggcgc gatgatgtcg
     1321 tcgctgcatt gcaggatcag cgtgggcgtg gtggcgcgcg gcaggatcgc gcggtgatcg
     1381 gagagaaagg tgacgcgcgc gaaatgccgc gcgatctcgg ggtcggtgcg gcagaagctg
     1441 ttggtcaact cgacgccgag ctcgggctgc gccggcgcgc ccatgatggc cggcgccatc
     1501 gtgctcgacc agccgaggta attgctttcg agcgtgcgta acaggtcttc gatatcctcg
     1561 cgcgtgaagc cgccgatata gtcgccgtca ttcacgtagc acggcgacgg ccccaccatg
     1621 atttgcgcgg caaacagctc aggcgcctta agactggcga gtagcccgat catcgcgctc
     1681 accgagtggc agacgaacac cacgggcgcg tcggcgactt ccttgacgat ttcgatcagg
     1741 tcgtcggcat aaccctgcag cgagtcgtat ttgccgatgt cgtaggcgga cagatccgac
     1801 gcgccgctgc cgacgagatc gaacagcacg gtgcgatact cgccgtggaa cgcgggcacg
     1861 agataccgcc acatggtctg atcgcagccg aagccgtgcg ccaggaccat ggtccgctcg
     1921 ccgctgccgg aaatctttac gttattgcgc tggctgatgc tcattgtcga gtcctgaggc
     1981 ctgccggcct aagcgggata cgcgaggccc ggcatggatg ggcaatgtta ccgcagtctt
     2041 gccgcgcggc cgggcgtgct atgcggcggg acgagcacga tgcgcctcac gccgttttga
     2101 tttcctgcgg cttcgcttca gccggctcga gtccaaaacg ttcgtattgc gaaatcacct
     2161 gcgcgccgaa caggagcaag gtagcgagcc actccaggct gaacaggatc acgatcgcca
     2221 tggtgagcga accgtacacc acgctcacct gggagagggt ggcaaaatac cagaccagca
     2281 catgccgcgt aatctcccac aacacggccg caacaatgcc gccgaacagc gcatgtttga
     2341 gcgacggctg cccgaccggc atgaccatat agatcgacgt caacacaaaa atctcgcccg
     2401 cgagaccaag cagatacaac accacacgcg acaatccctg caacgaaaca tgcaggccga
     2461 acagatcgat accttcggcg cccacagcct ccagcccgct cgatacgaat gtcacgatca
     2521 ataatccgac gccgagaaaa aggatgtagc aatagggcag cagcgcggag agcaaaaagt
     2581 gccgccgccg caccaccacg cgatgcacga aaatcaacga catcgcattc tcgagcacgg
     2641 tgaaggcgag cgagctgaaa aagatcatcg tcagcagcag aacccagccg atcaccgcgc
     2701 gatgcgccag gaagttggcc aactcgtgta ccagcgccgt ggattgcccc ggcacgagcc
     2761 actgcagcag atgagccagc gccgccagca gaacgtgctg gggtacgagc cgtgacaaga
     2821 cgatcacgat caggatcatc agcggcacaa tcgacaatag cgcgtaatac gcgaccgccc
     2881 ccgcgagcag caacccctgg ttcgcgcgga aagctttcag cgtctgcagg atgaacgtgc
     2941 cgggatgttt cgcgacatac agcgcgtgtt cgtcgatgat ggattgcatg gtgcctcaca
     3001 ttgcgcgtca ccgcgtctgc tgggaaagcc gcaggtttcg gcgcttaccc tgtatttgaa
     3061 gcaagaacgc agccacctcg tcaacatatt ggaatagatg ggccgtgggg cgtcgggaat
     3121 gtagtttgct ctgcatcgac agcagaatgg cccatccaat cacaatgaac gacgcgactg
     3181 atcaagccga actcgctgac ctcgtggtcg cgcgcccgga gggtttgtat tgcccgcctg
     3241 gcgacttcta catcgaccca tggcaacccg ttgagcgggc cgtgatcacg cacgcgcatt
     3301 ccgatcatgc gcgctttggg catcggcact atctcgcgtc gcgggccggc gcgaacgtgc
     3361 tgctttcgcg cctccccggc atcacgctgc aaacccttgc atacggcgag cggctcgaga
     3421 ttggcggcgc cggcgtgtcg ctccatcccg cgggacacgt gctgggttcc tcgcaactca
     3481 ggatcgagca caaaggccgc gtgtgggtcg cctccggcga ttacaagctc gatcccgatc
     3541 caacctgcga tgcgttcgaa ccggtgcgtt gcgagacctt tattaccgaa tcgaccttcg
     3601 gtttgccgat ctatcgatgg gatgcgccgc aaacgatctt cgacggcatc gattcgtggt
     3661 ggcgtcataa cgcggcagaa gggcgcgcct cggtgctgtt ctgctattcg ttcggcaagg
     3721 cgcaacgcgt gctcgccagc gtcgatgcgg ggatcgggcc gctcttctgc cagggcgcgg
     3781 tcgagccgct caagcgggcc tatcgggacg ccggcgtgcg cttgccagcg gtgcgtcccg
     3841 tcagcgaaat tcaggtgaaa gataaagcgg ctttcagaca cgccctgatc gtggcgccgc
     3901 cgtcggcgca aggcagcgcc tggctcaaac gtttcggcga ctatagcgat gcgttcgctt
     3961 cgggctggat gcgtctgcgc ggcgcgcggc gcagacgtgg cgtcgatcgc ggcttcgtgc
     4021 tgtcggatca cgcggattgg cccagcctgc aaagcgcgat tcaggcgaca ggggctgaac
     4081 gcgtaatcgt tacgcatggt tcggtcgaac cgatggtgcg ctggctgcgc gagcagggcc
     4141 tcgaggcagg cgcgtttgca acgcaatacg gtgacgacac ggttgaagcg gacgccgcgg
     4201 gtcattcatg aaacgcttcg ccgcgctcta taccgcgctc gacgcgacga cctccacgca
     4261 cgacaaactc gatgcgctca ccagctattt cgccgccgcg gcgcccgagg atgccgcgtg
     4321 ggcgtcgtac tttctcgccg gcggcaagcc gcgccaatcg gtgccgacgc ggcttctgcg
     4381 cgagatcgcc cgtgaacgcg ccggtctgcc ggagtggctc ttcgaggagt cgtatcacgc
     4441 agtcggcgat ctggctgaaa ccatcgcgca tatcctgccg cccgcgcaac gcagcaccga
     4501 actcggcctc acgcaatgga tcgagcaacg cgtgctgacg ctacgcggcg tggcgccgga
     4561 agaattgcgc gtgcgactgc tcagctattg ggacgaactc gactggggcg gccgctttct
     4621 gctgaccaaa ctgatcggcg gcggctttcg ggttggcgtg gcgcggcaac tggtggtgcg
     4681 ggcgctggcg tcagtggcgg gcgtcgatca taagcggatc gcgcagcgca tggtcggctg
     4741 gaccgactcg caactgaaac cggatgcggc acggtatctg cgtttgatcg cgccggagcc
     4801 aggcggtgac gcgacacaca gcgagccaac gcatgacagc gatctcggcc tgccttatcc
     4861 gttctttctc gcccatccct tgcaggccga tccgacaacg ctcggcgagc cgtcgaactg
     4921 gcagatcgaa tggaagtggg acggcattcg cgcgcaattg gtgaaacgcg gcggccgcgt
     4981 gtggctatgg tcgcgcggcg aggatctgat tacggagcgt ttccccgaag tggcggcact
     5041 cggtgaagcg ttgcccgacg gtttcgtgat cgacggcgaa atactcgcgt gggagccggg
     5101 cgcggcgtcg ccgttgccgt tcgcgcggct gcaaccgcgg atcgcccgca aaaccttgac
     5161 caaaaaaatt ctcgccgatt ctcccgccac gctgctcgcc tacgatctgc tcgaagcgct
     5221 cggcaaagat ctgcgcatga cgccactggc agagcggcgt gcgcaactcg atgggctcgc
     5281 aacttcattg gacggcacat tcgcaggcga tctcttacgc gtgtcaccgg tgatttccgc
     5341 gtcggactgg gcggcgcttg cgaagctgcg cgacgagagc cgggcacgcg gcgtggaagg
     5401 tctgatggtg aaacaacgcg aatcgatgta cggggtgggc cgcaccaaag cgtccggcac
     5461 atggtggaag tggaagatcg atccctatgc ggtggatgcg gtgctggtct acgcccaacg
     5521 cggtcatggg cggcgtgcga gcctgtacac cgacttcacc ttcgccgttt gggacgaagc
     5581 gaatggagtg cgcacgctcg tgccgtttgc caaggcctat tccggcctga ccgatgagga
     5641 aatgcgccag gtggacgcga tcgtacgcaa gaccacgat
//
LOCUS       DATASH010000091         6444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2978398,
            whole genome shotgun sequence.
ACCESSION   DATASH010000091 DATASH010000000
VERSION     DATASH010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6444
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2978398"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..1679)
                     /locus_tag="VHV99_02775"
     CDS             complement(219..1679)
                     /locus_tag="VHV99_02775"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HEX3634407.1"
                     /translation="MKKKLTTAFGAPVVDNQNIKTAGPNGPALLEDVWFLEKLANFDR
                     EVIPERRMHAKGSGAFGTFTVTHDITKYTRAKIFSEIGKKTEMFARFSTVAGERGAAD
                     AERDIRGFALKFYTEEGNWDMVGNNTPVFFLRDPLKFPDLNHAIKRDPRTGLRSADSN
                     WDFWTQLPEALHQVTVVMSDRGIPATFRHMHGYGSHTYSFISAKQERFWVKFHFRTQQ
                     GIRNLTDAEAEALIGRDRESHHRDLYEAIERHEFPRWTLLVQIMPEKDAATYKVNPFD
                     LTKVWSQRDYPLIEVGVMELNRNAENHFADVEQAAFSPANVVPGISFSPDKMLQGRLF
                     SYSDAQRYRLTVNYHQIPVNAPRCAKHVHSYHRDGLMRVDGNMGGATSYDPNTRGEWP
                     EQPDFREPPLAITGAADHWNHRVDDDYYSQPGDLFRMMTPTHQQTLFDNTARAMVGVS
                     APVRNLHIEHCTKADPAYGAGVAAAIERLEKEAAQA"
     gene            complement(1809..2360)
                     /locus_tag="VHV99_02780"
     CDS             complement(1809..2360)
                     /locus_tag="VHV99_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007582862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX3634408.1"
                     /translation="MQSDIRNAPPASELDDDTFEFANAVFDLARAGDSETLGALLGKG
                     LPPNLRNHRGDSLLMLAAYHGHLETVRVLLRHHSDPDLRNSNGQTPLAGAAFKGDLPM
                     IKLLLAYGADIEGASPDGRTALMIAAMFDRTLIVEYLVSHGANLDATDASGVSATDAA
                     HRMGASKVLALLQELKSASLNAR"
     gene            complement(2569..3465)
                     /locus_tag="VHV99_02785"
     CDS             complement(2569..3465)
                     /locus_tag="VHV99_02785"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3634409.1"
                     /translation="MFDLNEIYLFVEVIRAGSFAEAARRIGMPANTVSRRVQQLENQL
                     HSRLILRTTRRLMLTAVGQTFYERCARNVEELVLAGQQVADTDPEPTGPLRVALSPDF
                     FEILPISWVTGFLARYPGISLELVIDNAYLDLFASGVDLALRPESLLDENATRRVLRR
                     SRRRLVASPKYLEAYGTPACFDELGQHDCLVVSRNSGPDTWCLNGPEGRVEVRVRSRF
                     LASSNAPVRQAAVEGLGIAFLYDLYAKRDIEGGSLVPVLDQYRSDPSNYCAVFPGHRH
                     IRRVATTFAEAVQKQLIETYQE"
     gene            complement(3595..4779)
                     /gene="pobA"
                     /locus_tag="VHV99_02790"
     CDS             complement(3595..4779)
                     /gene="pobA"
                     /locus_tag="VHV99_02790"
                     /EC_number="1.14.13.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018659 - 4-hydroxybenzoate
                     3-monooxygenase activity [Evidence IEA];
                     GO_process: GO:0018874 - benzoate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HEX3634410.1"
                     /translation="MKTQVAIIGAGPSGLLLGQLLAQAGIDNVILERQTPEYVLGRIR
                     AGVLEQGTVDLLREAGVGARLDQDGVVHEGVELAFAGRRVRIDLKALSGGKTVTVYGQ
                     TEVTRDLMEARARSGATSIYHVANVQIHDITGGSPYLTYERNGERRTLHCDYVAGCDG
                     FHGVSRQTIPAEVLRVYERIYPFGWLGLLSDTPPVHHELIYAHHPNGFSLCSQRSRTR
                     SRYYLQVPLTEKVENWSDERFWNELKNRLPDHLGDQLVTGPSLEKSIAPLRSFVAEPM
                     QYGCLFLVGDAAHIVPPTGAKGLNLAASDVNTLYRILIKIYRERRTDLLVRYSDIALR
                     RVWKAVRFSWSMTSLLHEFPDSDAFSRKIQEIERDYLTSSQAGLTTIAENYVGLPYEA
                     IA"
     gene            4978..5850
                     /locus_tag="VHV99_02795"
     CDS             4978..5850
                     /locus_tag="VHV99_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006400215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3634411.1"
                     /translation="MPVFELFGERQTWPTPDLLHCESIPERSRVHDWEITQHRHADLG
                     QFVYVQAGHAVLDVEGSVTEIDEPAMQIVPPMCVHGFRFSRDIAGYVITLALPLFDWL
                     QDSLGDAAPALLRADCYAVGADKPYLDQLCQRIDREYATVAPGRDFALHSLVGALGSW
                     VGRQQHEQREAEWRPDRGQQRTGSFSRLVEQHFREHWSVAQYAAELAITTAQLNNVCH
                     RSLGCTALEHVHHRVLLEAKRNLIYTTMTVAQIADLLGFSDVGYFSRFFRRLERVSPR
                     DFRQQQASYGRTPA"
     gene            complement(5944..>6444)
                     /gene="ssb"
                     /locus_tag="VHV99_02800"
     CDS             complement(5944..>6444)
                     /gene="ssb"
                     /locus_tag="VHV99_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX3634412.1"
                     /translation="LGADPEVRYLPSGDAVANIRLATTDRYKDKASGEMKEATEWHRV
                     SFFGRLAEIVSEYLKKGSSVYLEGRIRTRKWQAQDGTDRYSTEIVAEQMQMLGGRGGS
                     MGGGGDEGGYSRGEPSERSGGGGGGRAVSGGGASRGGSGGGGGSSRPSAPAGGGFDEM
                     DDDIPF"
BASE COUNT         1192 a         1954 c         2006 g         1292 t
ORIGIN      
        1 atacggcggc catccgtcag actcaactgc gtcggtgcgg ctcgcgggcc gaagcgctcc
       61 ggatcagcgt gtcgcatttc acggttctcg ctgctctgcc gaagtgtgtt tttttacacc
      121 gggtttgtct cgatccgaca cgcaccggat cggtgtttca gcaaccaaca cgcaatgttg
      181 cggtcgcgcc gatccggcga gatcacgcga gtgtctgctc aggcctgcgc ggcctccttc
      241 tcgagccgct cgatcgccgc ggccacgccg gcgccgtatg cggggtcggc cttcgtgcag
      301 tgctcgatgt gcagattcct gaccggcgcc gacacgccaa ccatcgcgcg cgcggtgttg
      361 tcgaacagcg tctgctggtg cgtcggcgtc atcatgcgga acaaatcccc cggctgggag
      421 taatagtcgt cgtcgacgcg gtggttccag tgatcggccg caccggtgat cgccagcggc
      481 ggctcgcgaa agtccggctg ctccggccac tcgccgcggg tattcgggtc gtaggacgtg
      541 gcgccgccca tgttgccgtc gacccgcatc agcccgtcgc ggtgatagct gtgcacgtgc
      601 ttcgcgcagc gcggcgcgtt gaccggaatc tgatggtagt tcacggtcag ccggtagcgt
      661 tgcgcgtcgc tgtacgagaa caggcggcct tgcagcatct tgtccggtga gaagctgatg
      721 ccaggcacca cgttggccgg cgagaatgca gcctgctcga cgtcggcgaa atggttttcg
      781 gcattgcggt tcagttccat cacgccgact tcgatcagcg gatagtcgcg ctgcgaccac
      841 accttggtca gatcgaacgg gttgacctta taagtcgcgg cgtccttctc gggcatgatc
      901 tgcacaagta gcgtccagcg cgggaattcg tggcgttcaa tcgcctcgta caggtcgcga
      961 tgatggcttt cgcggtcgcg gccgatcagc gcttccgctt cggcgtcggt caggttcctg
     1021 atgccctgct gggtgcggaa atggaacttc acccagaagc gctcctgctt cgcgctgatg
     1081 aagctgtacg tgtggctgcc gtatccatgc atatggcgga acgtcgccgg aatgccgcga
     1141 tcgctcatca cgaccgtcac ctggtgcagc gcttccggga gctgcgtcca gaagtcccag
     1201 ttgctatccg cgctgcgcaa gcccgtgcgc ggatcgcgct tgatcgcgtg attcaggtcc
     1261 gggaacttca gagggtcgcg caggaagaac accggcgtgt tgttgccgac catgtcccag
     1321 ttgccctctt cagtgtagaa cttcagcgcg aagccgcgga tgtcccgctc ggcgtccgcc
     1381 gcgccacgct cgccggcgac cgtcgagaag cgcgcgaaca tctcggtctt cttgccgatc
     1441 tccgaaaaga ttttcgcgcg ggtgtacttc gtgatgtcgt gggtcacggt gaaggtaccg
     1501 aatgcgccgg agcccttcgc gtgcatccgc cgctcgggga ttacctcacg gtcgaaattg
     1561 gccaactttt cgaggaacca gacatcctcg agcaacgcgg gcccgttcgg gccggcggtc
     1621 ttgatgttct ggttgtctac aaccggagcg ccgaacgctg tcgtcaactt ttttttcata
     1681 acgccattcc tttacgtgaa tcgaatgaaa ctgccgtggg gggtgcgata gaccaaacat
     1741 cagtagctgc gccgagccgc tcgcgttcaa gccgggctcg agcgcggttc gaacaggttc
     1801 aaaccagatc agcgtgcatt gagcgacgct gatttcaatt cctgcaaaag cgccagcact
     1861 ttcgaggcgc ccatccgatg ggccgcatcg gttgcactga cgcccgatgc atcagtagcg
     1921 tcgagattgg cgccgtggct gaccagatac tcgacgatca gggtgcggtc aaacattgcc
     1981 gcgatcatca gcgcggtgcg tccgtcggga gacgcgcctt cgatatcggc gccgtatgcg
     2041 agcagcagct tgatcatcgg cagatcgccc ttgaaggcgg cgcctgccag cggcgtctgt
     2101 ccgttgctgt tgcgcagatc cggatcggag tgatgccgaa gcagaacgcg caccgtttcc
     2161 agatgtccgt gatacgcggc gagcatcagc agactgtcgc cgcgatgatt acgcagattc
     2221 ggcggcaggc ctttgccaag tagcgcgccg agcgtttccg agtcgcctgc gcgcgcgaga
     2281 tcgaataccg cgttcgcgaa ttcgaacgta tcgtcgtcga gctcgcttgc gggcggggca
     2341 ttgcggatgt cggattgcac attagtactc ctttgaacgg ggtgactaga aggtggcttt
     2401 cgaatgattc caatctatcg aagcctgcgt gacgcgacaa gtcgcccaat atggatgaca
     2461 cagctccacg aatcgaagta gtgaaggtgt cgagaagacg acatgcaaaa tcgacatgac
     2521 agcagagaaa gggatacgtc tgctcgagcc gatcaagaaa acggcggcct attcctgata
     2581 tgtttcgata agctgtttct ggacggcctc tgcgaaagtc gtcgcgaccc gcctgatgtg
     2641 ccgatgaccg ggaaagacgg cgcaataatt cgatgggtcc gaccgatact gatcgagcac
     2701 cggcacgaga ctgccgcctt cgatatccct cttcgcgtat aggtcgtaga ggaaagcgat
     2761 gccaaggcct tcgaccgccg cttgtctgac cggggcgttg ctgctcgcga gaaaccggct
     2821 tcgcactcga acctcgacac gtccttcggg gccgttcaga caccacgtgt cgggcccgga
     2881 gtttcgtgag accaccagac agtcgtgctg gccgagttca tcgaaacacg caggcgttcc
     2941 gtaggcctcg agatatttcg gcgatgcaac gagccgcctg cgcgacctcc tcagtacacg
     3001 gcgcgttgcg ttttcgtcca gcaaagattc cggacgcaaa gcgagatcga ctccgctcgc
     3061 aaacaggtcg agatatgcgt tgtcgatgac gagctccaga ctgatgcccg gatagcgtgc
     3121 gagaaatccg gtcacccatg aaatgggtag gatttcgaaa aaatcggggg acaacgcgac
     3181 gcgcaacggg ccagtcggtt ccggatcggt gtcggcgacc tgttgcccag caagcaccag
     3241 ttcttcgacg ttccgcgcgc atcgctcgta gaatgtctgc ccgaccgcag tcagcatcaa
     3301 ccttctcgtg gttcgaagaa tcaaccggct gtgcagctgg ttttcaagct gctgaacgcg
     3361 tcggctgacc gtgttcgccg gcatcccgat acggcgtgcc gcttcggcga aactcccggc
     3421 tcggatcacc tcgacgaaca gataaatttc gttcaggtcg aacatacatt cctcatactg
     3481 gatgtgcttc cgccatccgg accgtctaat actcagcgga acggtggact aagccgaact
     3541 gtcgcggaaa tcgtcgaggc tggttggtga agctgatgtc ggcgcgcgat ggcgtcacgc
     3601 gatggcttcg tagggcaagc cgacgtagtt ctccgcaatc gtggtcaagc cggcttgaga
     3661 gctcgtcaga tagtcccgtt cgatttcctg gatcttgcgg ctgaacgcgt cgctgtcggg
     3721 aaattcgtgc agcagtgaag tcatcgacca cgaaaagcgg acagccttcc agacccggcg
     3781 caacgcgata tccgaatagc gcaccagcag atccgtgcgc cgttcgcggt agatcttgat
     3841 caggattcga tacaacgtgt tcacgtcgct cgcggcaaga ttgaggcctt tggctcccgt
     3901 gggcggcacg atgtgcgcgg catcgccgac caggaacagg catccatact gcatcggctc
     3961 ggccacgaag ctgcgcagcg gcgcgatgct cttttcgagc gaaggtccgg tgacgagctg
     4021 atcgccgaga tggtcgggca ggcggttctt aagttcgttc cagaatcgtt cgtcggacca
     4081 gttctcgacc ttttcggtca agggcacctg caggtaatag cggctacggg tgcgcgagcg
     4141 ctggctgcac agcgaaaagc cgttcggatg atgcgcatag atcagttcgt gatggaccgg
     4201 cggagtatcg gacagcaggc cgagccagcc gaagggatag atgcgctcgt agacgcgcag
     4261 gacctcggcg gggatcgtct gccgcgagac gccatgaaag ccgtcgcagc ccgccacgta
     4321 atcgcagtgc agcgtgcgcc gctcaccgtt cctctcgtag gtcaggtacg gggagccgcc
     4381 ggtaatgtcg tgaatctgga catttgctac gtgataaatg ctcgttgcac ccgaccgcgc
     4441 gcgcgcttcc atgaggtctc gcgtgacttc ggtctggccg tagacggtga ctgttttgcc
     4501 gcccgacaag gccttcaggt caatacgtac gcgccggccc gcgaaggcga gttcgacgcc
     4561 ctcgtgtacg acgccatcct gatcgaggcg ggcgccgacc ccggcttcgc gcagcagatc
     4621 gaccgtgccc tgttcgagca cgccggcccg gatacgcccc agaacgtact cgggcgtttg
     4681 ccgttcgagg atgacgttgt cgattccggc ttgcgccagt agctggccca gcagcaggcc
     4741 ggaagggccc gctccgataa tggcgacttg ggttttcatt gcgtgcctcc gggtttttta
     4801 ccgcctcgtg cgaatgcgat ggacgcgagg ttcaggtttc tgatgcccga attctttagc
     4861 actgcggagc tggcgggaag ggaggaatcg gcgttaaatt tggacttttg gaaagtcctg
     4921 aaccaggcgg ggggcgggat ggggcgagta agtcatcgac cgtcgggcgg aaccggcgtg
     4981 ccggtattcg agctcttcgg tgaacggcag acgtggccca cgccggatct gctgcattgc
     5041 gagtcgatac cggaacgcag ccgtgtgcac gactgggaga tcacgcagca ccggcatgcg
     5101 gatctcggcc agttcgtgta cgtgcaagcc ggtcatgcgg tgctcgatgt ggaaggcagc
     5161 gtcaccgaaa tcgacgagcc ggccatgcag atcgtaccgc cgatgtgtgt gcacggcttt
     5221 cgcttcagcc gcgacattgc cggctacgtg atcacgctcg cgctgccgct gttcgactgg
     5281 ctgcaggaca gcctgggcga tgcagcccct gctttgctgc gggcggactg ctacgcggtc
     5341 ggcgccgaca agccctacct cgaccagtta tgccagcgga tcgaccgcga gtatgccacc
     5401 gtagcgccgg ggcgcgattt cgcactgcat tcgctggtcg gcgcgctcgg cagttgggtc
     5461 gggcgtcagc agcacgagca acgcgaagcc gaatggcggc cggatcgagg acagcaacgt
     5521 acgggatcct tttcccggct ggtcgagcag cattttcgcg agcattggtc tgtagcccag
     5581 tacgctgcgg agctggccat cacgacggcg caacttaaca atgtctgtca ccggtcgttg
     5641 ggatgcactg cgcttgagca tgtgcatcat cgcgttctgc tcgaggcgaa gcgcaatctg
     5701 atctacacga ccatgacggt cgcccagatt gccgatttgc tgggcttttc cgacgtaggg
     5761 tatttttcgc gcttcttccg gcggctggag cgggtgagtc cgcgcgattt tcggcagcag
     5821 caggcgtcgt acgggcgaac gcccgcttga aatgttgcgg gtaaaggaac gccccgaatc
     5881 ccagacataa aaaaccccgc acaagggcgg ggttctatct ttacactttt tggatacggg
     5941 aactcagaac ggaatatcgt catccatctc atcaaagcca ccgccggccg gtgcgctcgg
     6001 gcgactcgag ccaccaccgc cgccgctgcc accacgcgaa gcgccgccgc ccgaaaccgc
     6061 acggccgcca ccaccgccgc cgctacgctc cgacggctca cctcggctgt aaccgccttc
     6121 atcgccaccg ccgcccatcg agccgccacg gccacccagc atttgcatct gctcggcgac
     6181 gatttcggtc gaataacggt cggtgccgtc ctgagcctgc cacttgcgag tgcggatgcg
     6241 cccttccagg tacaccgacg agcctttctt cagatattcc gacacgattt cagcaagccg
     6301 gccgaagaac gacacgcggt gccattcggt tgcttccttc atttcgccgg acgctttgtc
     6361 cttgtaccga tccgtcgtcg caaggcggat gttcgccact gcgtcgccgc tcggaagata
     6421 acggacttcc ggatcggctc cgag
//
LOCUS       DATASH010000092         4608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3572110,
            whole genome shotgun sequence.
ACCESSION   DATASH010000092 DATASH010000000
VERSION     DATASH010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4608
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3572110"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..860)
                     /locus_tag="VHV99_02805"
     CDS             complement(183..860)
                     /locus_tag="VHV99_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEX3634413.1"
                     /translation="MLTVHHLNNSRSQRVLWLLEELGVPYEIKRYERDPKTMLAPPEL
                     RDVHPLGKSPVITDDGQTLAESGAIIEYLVDRYGQGRFAPSAGTQERLRYTYWMHYAE
                     GSAMPPLLLKLVALRIAGAPMPFFARPIARKIASTLKSSFIDPQLKLHLGYINKELGA
                     SGWFAGNDFTAADVQMSFPLEAATARGGMEGQIPAVVDFLKRIHARPAYQRALERGGK
                     YELLGGD"
     gene            complement(969..2192)
                     /locus_tag="VHV99_02810"
     CDS             complement(969..2192)
                     /locus_tag="VHV99_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX3634414.1"
                     /translation="MAASFIEVVAQDGGRFNAYVARPAQGSGPGLVLLQEIFGINETM
                     KSMAGRFAEEGYVVLVPDLFWRIEPGISLGYGEADMKQALGYLGRFDVDLAVDDIAAT
                     VATLRTLPEQAGKVGTVGYCLGGKLAFLAAARTDVDCAVSYYGVGLETHLDEVPAVRC
                     PMVFHFPENDSHCPPDTRERISAALRTRAQIEQYVYPGCDHAFAAPSRPQYDKPATMM
                     AYSRTLALLRKVLGPVYDLNALWEAHCYYEFATRDVDAVMPTMVAQPYVNHVPTMTGG
                     VGHDKLKRFYTHHFVNSNPADTKLIPISRTIGADRIVDEFIFSCTHSCEIDWLLPGVA
                     PTGKYFEVPMLAVVCFRGDKLYNEHIYWDQASVLVQVGLLNPAGLPVAGIESARKLLD
                     ETLPSNQLMGDKSRV"
     gene            complement(2245..3885)
                     /locus_tag="VHV99_02815"
     CDS             complement(2245..3885)
                     /locus_tag="VHV99_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX3634415.1"
                     /translation="MEPSAHVDTFARDNLPPQDQWPVFLLDNPDVAYPARFNCATELL
                     DRTIEAGHRDRPAIWSDVDGKPQATTYGELLALVNRSAHVLVDEMGLQPGNRVLLRGP
                     NTLHMAVTALAALKAGLVVVPTMPLLRAKELKQIIEKAQVSAALCDARLTAELARCND
                     PQDEFYCAGLKQTLLFHDDSADSLDTLAINQPDRFTACDTAADDVCLIAFTSGTTGAP
                     KGCMHFHRDVLAMCDLFPRHVLKPSSSDIFCGTPPLAFTFGLGGLLCFPLRAGASTVL
                     IERLTPETLLQTVERFHATIMFTAPTFYRQMAPLIGHHDVSSLTKTVSAGEALPDSTR
                     RLWRDASGIDMIDGIGGTELIHIFISAQGDAIRPHAIGRAVPGYVVQAVDDAMQPVAP
                     GTIGKLAVRGPTGCRYLADERQAKFVRDGWNLPGDSVYLDEDGYVFYQARADDMIVSA
                     GYNISGPEVESVLMQHDAVAECGVIGVADETRGQIVKAFVVLNPGYTPDDKLVAQLQD
                     FVKNSVAPYKYPRVIAFIDSLPRTETGKLKRFELRTLR"
     gene            complement(3962..4357)
                     /locus_tag="VHV99_02820"
     CDS             complement(3962..4357)
                     /locus_tag="VHV99_02820"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX3634416.1"
                     /translation="MKKALLPAGWIKPRGYANGVAATGTQVFIAGQIGWDEQARFQTT
                     DFAQQAIQALRNVLAVLHEAGGAPEHLVRLTWYVTDKREYLASLKDIGRAFRELIGDY
                     DIAMSAVQVVALIEDEAKVEIEATAVIPQ"
     gene            complement(4354..>4608)
                     /locus_tag="VHV99_02825"
     CDS             complement(4354..>4608)
                     /locus_tag="VHV99_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HEX3634417.1"
                     /translation="DCEFSRPSQMGETITLALSVVALGRRSISIEIVGYCGDETRFRA
                     KQVLVTTSLERGTSIDIPADIASALATFAPQPESIEAHRS"
BASE COUNT          844 a         1385 c         1490 g          889 t
ORIGIN      
        1 catcaacggg ttgcccatcg gatgcaagcg ggtacaagca gggtacaagc ggatgccgga
       61 cgtgccgccg ctcagcgcga tgatgacaat ccgatgggca acccgttgat ggcaatgaga
      121 aatttagtct agcactcgcg gcttttggcc cacaactacg aattcctggg cggcgtttgc
      181 cgtcaatcgc cacccagtag ttcgtacttg ccgccgcgtt ccagcgcccg ctgataagcc
      241 ggacgcgcgt gaatgcgctt caggaaatcg accactgcgg gaatctgccc ttccatgccg
      301 ccgcgcgcgg tggcggcttc gagcggaaag ctcatctgaa cgtcggccgc cgtgaaatca
      361 ttgccggcaa accagccgct cgcacccaat tccttattga tgtaaccaag gtgcagcttc
      421 aactgcggat cgatgaagct cgatttcagc gtcgacgcga tcttgcgggc aatcggcctg
      481 gcgaagaacg gcatcggcgc accggcgata cgcagcgcga ccagtttcag cagcagcggc
      541 ggcattgccg agccttccgc gtagtgcatc cagtaggtgt agcgtagccg ctcctgcgtg
      601 cccgccgagg gcgcgaaacg gccttgaccg tacctgtcga ccagatactc gatgatcgcg
      661 ccggattcgg cgagggtctg gccgtcgtcg gtgatgacgg gcgacttgcc gagcggatgc
      721 acgtcgcgca actcgggcgg cgccagcatc gttttcgggt cgcgctcgta gcgcttgatc
      781 tcgtacggaa cgccgagttc ctcgagcagc cacaacacgc gttgcgagcg ggagttgttc
      841 agatgatgga cggtcagcat ggggcgaggg acgttggcct gtgaagtgtc acatcatagg
      901 gatgttgtgc cgaggtgtgg ctctagtcgc gagtcgcgag tcgcccggct tgcgttcctc
      961 gccgccgttc ataccctgga cttgtcgccc atcagctgat tcgacggcaa cgtctcgtcg
     1021 agcagcttgc gcgcgctttc gataccggct accggcagcc ccgcgggatt cagcaggccg
     1081 acctgtacca gcaccgaggc ctgatcccaa tagatgtgct cgttgtacag cttgtcgccg
     1141 cggaaacaca ccaccgccag cattggcact tcgaaatatt tgccagttgg cgcgacgccg
     1201 ggcagcaacc agtcgatttc gcagctatgc gtgcagctga aaatgaattc gtcgacgatg
     1261 cggtccgcgc cgatcgtgcg cgaaatcgga atcagctttg tatcggcagg attcgaattg
     1321 acgaagtgat gcgtgtagaa ccgcttgagc ttgtcatggc ccacgccacc cgtcatggtg
     1381 ggcacgtgat tgacataggg ttgggcgacc atggtcggca tgacggcatc cacgtcgcgc
     1441 gtggcgaact cgtagtagca atgggcttcc cacaacgcgt tcagatcgta gaccggaccc
     1501 agcaccttgc gcagcagcgc gagcgtgcgg gaataggcca tcatcgtggc gggcttgtcg
     1561 tactgcgggc gcgagggtgc ggcgaacgcg tgatcgcaac cggggtacac gtattgctcg
     1621 atttgcgcac gcgtgcgcaa tgccgcgctg attcgttcgc gcgtgtccgg cggacagtgc
     1681 gagtcgtttt cggggaagtg gaacaccatg gggcagcgca cggccggcac ttcatccaga
     1741 tgcgtttcga ggccgacgcc gtaatagctc actgcgcagt ccacgtcagt gcgtgccgcg
     1801 gcgaggaacg ccagcttgcc gccgagacag tagccgaccg tgcccacctt gccggcctgc
     1861 tcgggcaagg tgcgcaatgt cgcgaccgtt gccgcgatat cgtcgacggc gagatcgaca
     1921 tcgaagcgcc cgaggtagcc aagtgcctgc ttcatatcgg cttcgccata gccgagcgag
     1981 atgccgggct cgatccgcca gaacagatcc ggcacgagca ccacataacc ttcttccgcg
     2041 aagcgcccgg ccatcgactt catggtctcg ttgatgccga aaatctcctg cagcagcacg
     2101 agccccgggc ccgagccttg cgccggacgc gcgacgtagg cgttgaaacg gccgccatcc
     2161 tgggcgacga cttcgatgaa agaggcggcc atgcggtgtg tctccatgca aagtagttgc
     2221 cgggaaagag ggctacatga gggcttacct gagcgtacgc aattcaaagc gtttgagctt
     2281 gccggtttca gtgcgtggca gcgagtcgat gaaagcgatg acgcgtgggt acttatacgg
     2341 cgccacgcta tttttcacga aatcctggag ttgcgcgacc agtttgtcat ccggcgtata
     2401 acccgggttc agcaccacga aggctttgac gatctgcccg cgcgtttcgt ccgccacgcc
     2461 gatcacaccg cattcggcca ccgcgtcgtg ttgcatcagt acgctttcca cttccgggcc
     2521 ggagatgttg tagccggcgg agacgatcat gtcgtcggcg cgggcctgat agaacacgta
     2581 gccgtcttcg tcgagataga ccgagtcgcc gggaagattc cagccgtcgc ggacgaactt
     2641 cgcctgccgt tcgtcggcga gataacggca gccggtcggc ccgcgtaccg cgagtttgcc
     2701 gatcgtgcca ggggctacgg gctgcatggc gtcgtccacg gcttgcacca cgtagcccgg
     2761 caccgcgcgg ccaatggcat gcgggcggat cgcgtcgcct tgcgccgaga tgaaaatatg
     2821 gatcagttcc gtgccgccga tcccgtcgat catgtcgatg ccgcttgcgt cgcgccatag
     2881 gcggcgcgtc gaatccggca acgcttcgcc cgccgagacc gttttcgtca ggctcgaaac
     2941 gtcgtgatgc ccgatgagcg gcgccatttg ccggtagaac gtcggcgcgg tgaacatgat
     3001 cgtcgcgtga aagcgctcga cggtttgcaa caaggtctcg ggtgtgagcc gctcgatcag
     3061 caccgtcgac gcacccgctc gcagcggaaa gcacagcaag ccgccaaggc cgaatgtgaa
     3121 ggcgagcggc ggcgtgccgc aaaatatatc gctcgatgaa ggcttgagaa cgtgacgcgg
     3181 aaacagatcg cacatcgcca atacgtcgcg atgaaagtgc atgcagcctt tcggggcgcc
     3241 cgtcgtgccg ctggtgaagg cgatcaggca gacgtcgtcc gcggcggtgt cgcaggcggt
     3301 gaagcggtcc ggctgattga tggcgagtgt gtcgagcgaa tcggcggaat cgtcgtgaaa
     3361 caggagcgtc tgtttcaagc ccgcgcagta gaactcatcc tgaggatcgt tgcagcgcgc
     3421 gagctcagcg gtcaggcgtg catcgcatag cgcggcgctg acttgcgcct tctcgatgat
     3481 ctgcttgagt tctttcgcgc gcagcagcgg catggtgggc acgacgacga ggccggcctt
     3541 caacgcggcg agcgccgtca ccgccatgtg cagcgtattc ggtccgcgca gcagcacgcg
     3601 gttgcccggt tgcaaaccca tttcgtcgac caggacatgc gcgctgcgat tgaccagtgc
     3661 cagcagttcg ccataagtgg tggcttgcgg ttttccgtcc acgtccgacc agatcgccgg
     3721 acggtcgcga tgtcccgcct cgatggttct gtcgagcaat tccgtcgcgc aattgaagcg
     3781 cgccgggtag gcaacatccg ggttgtcgag cagaaagaca ggccactggt cctgcggcgg
     3841 aagattgtcg cgcgcgaagg tgtcgacgtg tgctgacggt tccatcatgg tctcccgggc
     3901 gttgagccgg ctaaatgcgc ttcgtttggc ttggtggctg ggtgcttcgt atgtcgtggg
     3961 cttactgagg gatcactgcc gtcgcttcga tttcgacctt ggcttcgtct tctatcagcg
     4021 cgacgacctg gacggcgctc atggcaatgt cgtagtcgcc gatcagttcg cgaaacgcac
     4081 ggccgatatc cttcaacgag gcgagatact cgcgcttgtc cgtgacgtac cacgtcaagc
     4141 gcacgagatg ctcgggtgcg ccgcccgctt cgtgcagtac ggcgagtacg ttccggagcg
     4201 cctgaatcgc ctgttgtgcg aagtcggtgg tctgaaaacg cgcctgttcg tcccagccga
     4261 tctggccggc gatgaacact tgcgtgccgg tggccgcgac gccgttcgcg tagccgcgcg
     4321 gcttgatcca accggcggga aggagagctt ttttcatgag cgatgcgcct cgatggattc
     4381 aggctgtgga gcaaacgtcg cgagcgcgct cgcgatgtcg gcgggaatgt cgatggaggt
     4441 gccgcgttcg agcgatgtcg tcacgagcac ctgttttgcg cgaaagcggg tttcgtcgcc
     4501 gcaatagccg acgatctcga tgctgatcga gcggcggccg agcgccacga cgctgagtgc
     4561 cagcgtgatc gtttcaccca tctggctcgg ccgcgaaaac tcgcaatc
//
LOCUS       DATASH010000093         2741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6737355,
            whole genome shotgun sequence.
ACCESSION   DATASH010000093 DATASH010000000
VERSION     DATASH010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6737355"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="VHV99_02830"
     CDS             complement(<1..593)
                     /locus_tag="VHV99_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3634418.1"
                     /translation="MPALNALKAFEAAGTTGSFTRAAELLNVTQSAVSRQVRQLEEQL
                     GETLFSRRHQHLVLTPSGRVLLRALQQAFDKIELTVRAISQNNDVNRLRINVPPTFAA
                     RWLVPRLGRLREAFPQLDLSITTRVDDGIADSNRLDCAVRFGDGEWSNLESTLLMHER
                     HIAVASPALLDREGVDAPVDLNRFTLLHVLSGEDQRYL"
     gene            959..2260
                     /locus_tag="VHV99_02835"
     CDS             959..2260
                     /locus_tag="VHV99_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX3634419.1"
                     /translation="MTETIWFLVVGGVLIFMGLASSTFKQLPISSAMVYLAIGFGLGP
                     FGIGLLKIDLLGEATLLRRITEVAMLVSLFAIGLRLRVPPTDRIWLLPVRLGLVAMVA
                     TVAIMTLAGVYLLHMNWGPALLIAAMLAPTDPVLAHDVQVQVPGDVDLLRFSLTGEGG
                     LNDGIALPFALLGIAVCNYEASPKQALSWHFAGDALWGVAGALAAGWLLGDLAVRLIA
                     WLRSRHGLALGPEGFYALGLIALAYGVAELIHTYAFLAVFAAGLAMRRVEQKASGDKS
                     AHEALGIIDAEDVGATASDPDRAHAFVAERVLGFTIELERIAEVAVMLMVGSVLANVW
                     DRLFTWQGAAVVGLLFFVARPVSVQASLLGSSATRSQRLLTSWFGIRGVGSFYYLLYA
                     LEHAPRSVAEPLAPLVLGVITASVILHGITSTPLMNRYGRS"
     gene            complement(2364..>2741)
                     /locus_tag="VHV99_02840"
     CDS             complement(2364..>2741)
                     /locus_tag="VHV99_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HEX3634420.1"
                     /translation="LDDLRMSSDRDGLKAMFRRAIPATAQDVVLVFVTATGMRDGRLV
                     QDVFMRKIFAKSVCGVPMSAIQITTAGAICAELDLFREGLLPQSGFIRQEQVPLEAFL
                     ANRFGQLYEGASTLGVVRQLHAA"
BASE COUNT          456 a          857 c          866 g          562 t
ORIGIN      
        1 aggtaccgct gatcctcgcc cgatagaaca tgcagcaagg tgaagcggtt caggtccacc
       61 ggcgcatcca cgccttcgcg atccagcaac gcgggcgacg ccaccgcaat atgccgctcg
      121 tgcatcagca aggtactttc aaggttgctc cactcgccat cgccgaaacg caccgcacaa
      181 tccaaccggt ttgaatccgc gatgccatcg tccacgcgcg tggtgatgct caggtcgagt
      241 tgcggaaatg cttcacgcag acgcccgagg cgcggcacca gccagcgcgc ggcaaaggtc
      301 ggcgggacgt tgatacgcaa gcggttgacg tcgttgttct gactgatcgc gcgaaccgtc
      361 agttcgatct tgtcgaaggc ctgttgcaac gcacgcagta aaacgcgacc gctcggcgtg
      421 aggacgagat gctgatgacg gcgggaaaac agcgtctcac cgagctgctc ctcgagttgc
      481 cggacctgcc ggctgaccgc gctttgcgtg acgttcagca actcggctgc gcgggtgaag
      541 ctgccggtgg tgccagccgc ctcgaaggct ttgagcgcat tcagcgcagg cattttacgt
      601 ttcacgatga tctgaacagt tcgttggctc gcgacgtgcc ggcgtgccgg agtgtgggcg
      661 agcgggtgcg cgcgatttta cccagccggc cgtctgcacc gtgtcgaacg atggcttcat
      721 catcaggcgg gcgctctgca ggcgcatcat tgtgcccgag cccgtggcgt tgcgcggctc
      781 gccagtgcac gaacaaaacc cagtgggttt cacgcggcga aactcattcg tgcactggca
      841 tcacacgctg cacatccgcg cccgcgcgcc ctctcccacg ctcttggaat cgctcagccg
      901 gtgcgggtgg cacgcccctt gctcctagtc gcgtacgctg cattcaactc aggctctcat
      961 gaccgaaacg atatggtttc tcgtcgttgg cggtgttctc attttcatgg ggcttgccag
     1021 ttcgacgttc aaacaactgc cgatcagcag cgccatggtg tacctcgcca tcggtttcgg
     1081 gctgggtccg tttggcatcg gcctgcttaa gatcgatctg ctcggcgaag ccaccctgct
     1141 gcgccggatt acggaagtcg ccatgctcgt gtcgctgttc gcgatcgggt tgcgcctgcg
     1201 tgtgccgccc accgaccgca tctggctctt gcccgtgcgc cttggtttgg tcgcgatggt
     1261 ggcaaccgtc gccatcatga cgctcgcagg cgtctatctg ctgcacatga actggggacc
     1321 cgcgctgctc attgccgcga tgctcgcccc caccgatccc gtgctcgccc atgacgtgca
     1381 agtgcaggtt cccggcgatg tcgacttgct gcgcttctcg ctcaccggcg aagggggctt
     1441 gaacgacggt atcgcgctgc cgtttgcgtt gctgggcatt gcggtgtgca actacgaggc
     1501 gtcgccaaag caggcgctaa gctggcattt cgccggcgat gcgctctggg gggtcgccgg
     1561 tgcgttggcc gcaggctggc tgctcggaga tctcgcggtg cggctgattg cgtggttgcg
     1621 cagccgccac ggcctggcgc tcggacctga gggtttttat gcgctggggt tgatcgcact
     1681 cgcatatggc gtcgccgaac tgatccacac gtatgcgttc ctcgccgtat tcgcggcggg
     1741 cctcgcgatg cggcgcgtcg aacagaaggc cagcggcgac aagtcggcgc acgaagcgct
     1801 tggcatcatc gatgccgagg atgtcggcgc taccgcctcc gatcctgatc gtgcgcatgc
     1861 gtttgtcgcg gaacgggtac tcggcttcac gatagaactc gaacggatcg cggaagtcgc
     1921 ggtcatgctg atggtgggca gtgtgctcgc caatgtctgg gatcggctgt ttacgtggca
     1981 aggcgccgcg gtggtcggcc tgttgttttt cgtggcgcgc ccggtgtcgg tgcaggcttc
     2041 gttgctgggg tcatcggcga cgcgctcgca gcgcctgctc accagttggt tcgggattcg
     2101 cggcgttggg tcgttttatt acctgctgta cgcactggag catgcgcctc gcagcgtcgc
     2161 tgaaccgctt gccccgctgg tgctgggagt gatcacggcg tcggtgatcc tgcatggcat
     2221 tacgtcgacg ccactgatga atcgatacgg aagaagttga tccgtcggcc tgctgcgttg
     2281 gtcaggccaa agggatcgtt gatcgctcgt tgcgcctcgc gctcaaaacc agccgaccgc
     2341 ggggcatcat tgcgagcgaa acctcaggca gcgtggagtt gccggacaac gcccagtgtg
     2401 ctcgcgcctt cgtacaactg cccgaaccgg ttcgccaaga acgcttcgag cggcacctgc
     2461 tcctgccgga taaagccgct ctgcggcaac aggccttcac ggaacagatc gagttccgca
     2521 cagatcgcgc cggcggtcgt aatctgaatg gcgctcattg gcacaccaca aacgctcttc
     2581 gcgaagattt tgcgcatgaa gacatcctgc acaagacggc catcgcgcat gccggtcgct
     2641 gtgacgaata ccagtaccac atcctgcgcc gtagccggaa tggcacgccg gaacatcgcc
     2701 ttcaatccgt cacggtcact cgacatgcgc aagtcgtcga g
//
LOCUS       DATASH010000094        12615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6737401,
            whole genome shotgun sequence.
ACCESSION   DATASH010000094 DATASH010000000
VERSION     DATASH010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 12615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12615
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6737401"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..102)
                     /locus_tag="VHV99_02845"
     CDS             complement(<1..102)
                     /locus_tag="VHV99_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HEX3634421.1"
                     /translation="MVVAVYPGTFDPLTRGHEDLVRRASSIFDTLVVG"
     gene            complement(214..828)
                     /gene="rsmD"
                     /locus_tag="VHV99_02850"
     CDS             complement(214..828)
                     /gene="rsmD"
                     /locus_tag="VHV99_02850"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEX3634422.1"
                     /translation="MPRSAPSRAHGASSKGGKPHAIRIIGGDWKRTPLPVLDLDGLRP
                     TPDRVRETLFNWLGQRLDGQRCLDLFAGSGALGFEAASRGAARVLMVERNARAASQLR
                     ANQERLSARTIEIVEADGLRLAASLPPGSFDVVFLDPPFGDDLLDKALALAVSLLSPE
                     GLLYVEAGAALALDGSETLAGWEIVRQGKAGAVHFHLLQRENKE"
     gene            1069..2235
                     /gene="ftsY"
                     /locus_tag="VHV99_02855"
     CDS             1069..2235
                     /gene="ftsY"
                     /locus_tag="VHV99_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018434695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEX3634423.1"
                     /translation="MFSFFKRFKGSKESDNAPEESQTAPEALAPEPAVEAPVAPVAPS
                     APRPVAPVNVEPPPATTLPASQPGAEEPALQAVEIVPPPMQDASAKRSWLTRLKTGLS
                     KTSSSLTGIFVGAKIDEDLYEELETALLMSDAGVEATEFLLESLREKVRAERLTDPQQ
                     VKTALRTLLIDLLKPLEKSLMLGRAQPLVMMIAGVNGAGKTTSIGKLAKHLQSFEQSV
                     LLAAGDTFRAAAREQLAIWGQRNNVTVVSQESGDPAAVIFDAVGAARARKIDVMMADT
                     AGRLPTQLHLMEELRKVKRVIGKAQEGAPHEVLLVIDANTGQNALAQVKAFDDALGLT
                     GLIVTKLDGTAKGGILAAIARQRPIPVYFIGVGEKVEDLQPFSAEEFSDALLGG"
     gene            complement(2367..3011)
                     /gene="maiA"
                     /locus_tag="VHV99_02860"
     CDS             complement(2367..3011)
                     /gene="maiA"
                     /locus_tag="VHV99_02860"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HEX3634424.1"
                     /translation="MKLYSYFRSSASYRVRIALNVKNLPYDYVPVHLVRDGGEQLKPA
                     YRKVNVDGIVPTLVDGSEVMPQSLAIIEYLEETHPEPPLLPGTPTERAYVRSVALQVA
                     CEIHPLNNLRVLKYLKHTLRVDDDAKDAWYKHWVETGFATLEAHLAGDHRTGKLCFGD
                     TPTLADACLIPQVFNAQRFKVDTAKFPTIQRIYDHAMQLDAFARAAPGVQPDAE"
     gene            complement(3037..3735)
                     /locus_tag="VHV99_02865"
     CDS             complement(3037..3735)
                     /locus_tag="VHV99_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX3634425.1"
                     /translation="MSYVFAPAPVVAVPVVGSSEQFAVRRIYCVGRNYEAHAREMGHD
                     PDREPPFFFTKPADAVLYVAPGATGEFPYPSQSKNVHFEMELVAAIGKGGKNIPAESA
                     LDHVYGYALGLDMTRRDLQAEAKKLGRPWDTAKGFDQSAPLGPIHPVTTIGHVGKGAI
                     WLSVNGEEKQSSDVSQLIWSVAETVAYLSTLFELQPGDLIFTGTPEGVGAVVQGDLMK
                     GGVDGLGEFSVRVV"
     gene            4223..5194
                     /gene="ybiB"
                     /locus_tag="VHV99_02870"
     CDS             4223..5194
                     /gene="ybiB"
                     /locus_tag="VHV99_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HEX3634426.1"
                     /translation="MTDSADTANPFPCARYIKEIGRGPNGARALTAEDTRALYTAMLE
                     GRVSDLELGAILLAYRVKGETANELAAMLAGAHASFEPIHLPHAAFRPVSIPTYNGAR
                     KQPNLVPLLALLLAREGVPVLVHGVTEDPGRVTSAEIFTHLQIPHAQSHADIEDGLAE
                     RRLAFAPIEVLAPKLARLLSLRRRMGVRNSTHTLVKILQPFAPAGLRLVNYTHPPYRD
                     SLTQLFNTHPDAAAGGALLARGTEGEAVADTRRQVQVDWLHDGICETRVPHERSSSDA
                     PEVDLPEARDAPTTAAWISAVLRGEAPVPSAIERQVQLIADVAKTPL"
     gene            5723..6706
                     /locus_tag="VHV99_02875"
                     /pseudo
     CDS             5723..6706
                     /locus_tag="VHV99_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019254946.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
     gene            complement(6906..7289)
                     /locus_tag="VHV99_02880"
     CDS             complement(6906..7289)
                     /locus_tag="VHV99_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX3634427.1"
                     /translation="MAAKIIEAIRGLERERFRAMVDGDGQSLDTLLADNVSYVHTNGK
                     RETKRQFIDGITAGRRRYRQIEVQSQEVLPVGRETCVVTGRALIEMEANNGALLFPIA
                     YTAIHTQEDGQWRLIAWQATRCAIE"
     gene            complement(7333..7896)
                     /locus_tag="VHV99_02885"
     CDS             complement(7333..7896)
                     /locus_tag="VHV99_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HEX3634428.1"
                     /translation="MNNKKDALRGLALKGTCTAVLVSASGMAAADQFGVQFGGGTGDH
                     HFKKYDLGFVWDPNLTWGQIGDWHFALIGEAHAALWHTGEANVHQNIGEFGVTPIIRF
                     INGTGAVRPFIEAGAGVRLLTSPTVSTDYTYSTAFQFTPMAGVGVQLGSHQQYQVGYR
                     FQHISNGGIKEPNPGINFHQLYLQYNF"
     gene            8260..9195
                     /gene="rpoH"
                     /locus_tag="VHV99_02890"
     CDS             8260..9195
                     /gene="rpoH"
                     /locus_tag="VHV99_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEX3634429.1"
                     /translation="MSHAMTLPSTLSPSSAKAASAGALALSHSSLLPGQLGNIDAYIQ
                     AVNRIPMLTPAEERQFATEFREQDNLESARRLVLSHLRLVVSIARNYLGYGLPHADLI
                     QEGNIGLMKAVKRFDPEQNVRLVSYAMHWIKAEIHEYILRNWRMVKVATTKAQRKLFF
                     NLRSHKQGLGAFTPDEIEGLAKELNVKREEVSEMETRLSGGDIALEDQVEDGEESYAP
                     IAYLADSHSEPTAVLASRQRDKLQSDGIASALDALDARSRRIIEARWLKVEDDGSGGS
                     TLHELADEFGVSAERIRQIEASAMKKMRGALTEYA"
     gene            9548..9808
                     /locus_tag="VHV99_02895"
     CDS             9548..9808
                     /locus_tag="VHV99_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010101551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634430.1"
                     /translation="MTIALNRHSNPRPSLRKRLAAWVRGPTLARDIAIVLAIKFALLM
                     ALKYTFFNHPQAEHMSLPPEQVAQALLSTPASHPSQGDQHAR"
     gene            9798..11387
                     /locus_tag="VHV99_02900"
     CDS             9798..11387
                     /locus_tag="VHV99_02900"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEX3634431.1"
                     /translation="MPVSEVVELSRLQFAITALYHFLFVPLTLGLSWLLVIMESVYVM
                     TGKQIYKDMTQFWGKLFGINFAMGVTTGLTLEFQFGTNWSYYSHYVGDIFGVPLAVEG
                     LMAFFLESTFVGLFFFGWNRLSKVQHVIVTFLVALGSNLSALWILVANGWMNNPVGAT
                     FNYQTMRMELDSIFSVLFNPVAQVKFVHTVSAGYVTASMFVLGVSSWYLLKRRDTEFA
                     LRSFAIAAGFGLASTLCVIVLGDESGYRTGEVQQVKLAAIESEWETAPAPAPFTIIGI
                     PNQKEERTDYAIRIPYALGLIATRSIDEPVVGLKDLMAQNEVRIKNGMLAYAALQQLK
                     LGDATDEAKAAFDAHKADLGYGLMLKQFTPNVTDATPDQIVQAAKKTIPPVAPVFFSF
                     RLMVGLGILFLATFVLAFWFCAQRSLLLEKRRWFLRWAVWAIPLPWLAAEFGWIVAEV
                     GRQPWTIAGILPTALSASSLTPSDLYLSIAGFVVFYTVLFIIEIMLMFKYARLGPSSL
                     HTGRYHHEQPLNQPLPTNTAA"
     gene            11422..12558
                     /gene="cydB"
                     /locus_tag="VHV99_02905"
     CDS             11422..12558
                     /gene="cydB"
                     /locus_tag="VHV99_02905"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010101549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEX3634432.1"
                     /translation="MDYATLKLIWWALIGVLLIGFALTDGFDMGAAILLPFIARTDGE
                     RRIVVNTVGATWEGNQVWLITAGGAMFAAWPLVYAASFSGFYFAMLLVLFSLFFRPVG
                     FDYRGKREDPRWRSAWDWALFVGGFVPALVFGVAFGNLLQGVPFSFDTDLRVTYHGSF
                     FALLNPFAVVCGLVSVSMLAAHGAAFVKMKADDIVAERASLALRIASLAAVVLFLLAG
                     ALIATMIGGYQIIDAPPLDTVANPLLKNVIGAPGLWLTNYANYPWMVAAPAAGVIGGV
                     LALLLARSRFEKSAFLSTSLMIIGVILTAAFSMFPFIMPSSLDGRSSLTVWDSTSSRM
                     TLQIMLIAVIIFLPIVLIYTSWVYRVMRGKVTAAALEENHHSMY"
BASE COUNT         2369 a         4030 c         3781 g         2435 t
ORIGIN      
        1 gcccaccacc agcgtgtcga aaatgctcga cgcgcgtcga acgaggtctt cgtgaccgcg
       61 cgtcagcggg tcgaacgtgc ccgggtacac ggcgactacc atgagacttc tcctctcttc
      121 agacaaaagg gcacgtcaaa gcgcccacgc gacgcatttg gcaccgaccg cacgcgtgac
      181 ggcaacccgc ccgctgcttg ttttgaacgc gcattattcc ttattttcgc gttgcagcaa
      241 atgaaagtgg acagcacccg ccttgccttg ccgcacgatt tcccagccgg cgagggtctc
      301 gctcccatcc agtgcgagcg cagcgcccgc ttccacatac aacaaacctt ccgggctcaa
      361 cagcgacaca gccagcgcga gcgccttgtc aagcagatcg tcgccaaacg gcggatcgag
      421 gaaaaccacg tcgaacgatc ctggtgggag acttgccgcg aggcgcaggc cgtcggcttc
      481 cacgatttcg atcgtacgcg ccgacaggcg ttcctggttc gcgcgcaatt ggctggcggc
      541 ccgcgcattg cgctcgacca tcaacacccg cgccgcgccg cgtgaagcgg cctcgaagcc
      601 aagcgcgccg ctgccggcga acaggtcgag gcagcgctgc ccgtccagcc gttggccaag
      661 ccagttgaac agcgtttcgc gcacgcggtc gggcgtgggg cgcaggccgt cgaggtcgag
      721 tacgggcagc ggcgtgcgct tccagtcacc gccgataata cgaatggcgt gcggcttgcc
      781 gcctttggac gaagcgccgt gagcgcgtga aggagcagaa cggggcatgc ggattcgatt
      841 acgtgataag tgcgcccgcg acgccgatgc gtgcgggccg gagcgccaac gttaccacag
      901 gggcgcacgg cgtccagcct ggccgttcgg ccaatacgac gggcggcggc gcgcctgata
      961 aaatgtgcgg cttccagcgc cttgcatccc gcatcgcaac gctgcctgcc gctcccacgg
     1021 gcttgtcccg gctgcgcgca tcggcgcgcg ctctcaccat gccagatcat gttcagcttt
     1081 ttcaaacgat tcaagggttc gaaagagtcc gataacgcgc cagaagaatc gcaaaccgcg
     1141 ccagaagcct tggcgccaga gccggccgtt gaggcgccag tagcgcccgt tgcgccgtcg
     1201 gcgccgcggc cggttgcgcc agtgaacgtc gaaccgccgc cggcaaccac gctgcccgca
     1261 agccaacccg gagccgaaga acccgcgctc caggccgtcg aaatcgtccc accgcctatg
     1321 caggacgcga gcgcgaagcg ctcctggctc actcgcctga agaccggctt gtcgaaaacg
     1381 agttcgagcc tgaccggaat tttcgtcggc gcgaaaatcg acgaggatct gtacgaagaa
     1441 ctcgaaaccg cgctgctgat gtcggatgcg ggtgtcgaag cgaccgaatt cctgctcgaa
     1501 tcgctgcgcg agaaagtgcg cgccgagcgc ctcaccgatc ctcagcaggt gaagacggcg
     1561 ctgcgcacgc tgctgatcga cctgctcaag ccgctggaaa aatcgctgat gctcggccgc
     1621 gcgcaaccgt tggtcatgat gatcgcgggt gtcaacggtg cgggcaagac cactagcatc
     1681 ggtaagctcg ccaagcatct gcaaagcttc gagcagtcgg tgctgctggc tgcgggggac
     1741 accttccgcg ccgccgcgcg cgagcaactc gcaatctggg gccagcgcaa caacgtgacg
     1801 gtcgtgtcgc aggaaagcgg cgacccggca gccgtgattt tcgacgcggt cggcgccgcg
     1861 cgtgcccgca agatcgacgt gatgatggca gacaccgccg gccgtctgcc gacccaactg
     1921 catctgatgg aagagctgcg caaggtgaag cgcgtgatcg gcaaggcgca ggagggcgca
     1981 ccgcatgaag tgctgctcgt gatcgacgcc aacaccggcc agaacgccct tgcgcaagtg
     2041 aaagccttcg acgacgcgct cggcctcact ggcctgatcg tcaccaagct cgacggcacg
     2101 gcgaagggtg ggattctcgc ggcgatcgcg cggcagcgtc cgattccggt gtacttcatc
     2161 ggcgtcggcg aaaaagtgga ggacttgcag ccgttcagcg cggaagagtt ttcggacgcg
     2221 ttgctgggcg gttgaaccca gcaacgtatc caggccaggc aggcaacaag ggcgctccgc
     2281 ggtgaactgc ccctgcaaag tcggacatcg atccaacctt cggggggcgg ttcacggagc
     2341 gcccttcctt cattaagcca cccgcgtcat tccgcgtccg gctgcacgcc aggcgccgca
     2401 cgcgcgaacg cgtcgagttg catcgcgtga tcgtagatgc gctgaatcgt cggaaatttc
     2461 gccgtgtcga ccttgaagcg ctgcgcgttg aacacctgcg gaatcaggca ggcgtcggcc
     2521 agtgtcggcg tgtcgccaaa gcacagcttg ccggtacggt gatcgcctgc cagatgtgcc
     2581 tcgagcgtcg caaagcccgt ctcgacccaa tgcttgtacc acgcgtcttt cgcgtcgtcg
     2641 tcgacgcgca gcgtgtgctt caggtacttc agcacgcgca gattgttcag cgggtgaatc
     2701 tcgcacgcca cctgcaacgc taccgagcgc acataggcgc gctccgtcgg cgtgccgggc
     2761 aagagcggcg gctcgggatg cgtttcctcc agatactcga tgatcgcgag cgactgcggc
     2821 atcacttcac tgccatcgac caacgtgggc acgatgccgt ccacgttcac cttgcgatac
     2881 gccggcttca gctgctcgcc gccgtcgcgc acgaggtgca ccggcacgta gtcgtaaggc
     2941 agattcttca cgttcagcgc gatgcgcacg cgataggatg ccgagctgcg aaaatagctg
     3001 taaagcttca tgttgtgtgt ctcctccgtg cgcgcttcag acgacgcgta cgctgaactc
     3061 gccaagcccg tccacgccgc ccttcatgag gtcgccctgc accacagcgc caacgccctc
     3121 cggtgtgccg gtgaaaatca gatcgcccgg ttgcagttcg aacagcgtcg acagataggc
     3181 gactgtctcg gccaccgacc agatcagttg tgacacgtcc gagctctgtt tttcctcacc
     3241 gttcaccgac aaccagatcg cgcccttgcc gacgtggccg atcgtcgtaa ccggatgaat
     3301 cgggccgagc ggcgccgact gatcgaaacc cttcgcggtg tcccagggac ggcccagttt
     3361 cttcgcttcg gcctgcaggt cgcggcgcgt catgtcgaga ccgagcgcat agccgtacac
     3421 gtggtcgagc gcgctttccg ccggaatgtt cttgccgcct ttgccgatcg ccgctaccag
     3481 ttccatctcg aagtggacgt tcttcgattg cgacggatag ggaaattcgc cggtcgcgcc
     3541 aggcgcgacg tacagcaccg cgtcggctgg cttggtgaag aagaacggcg gttcgcggtc
     3601 gggatcgtgc cccatctcgc gcgcgtgcgc ctcgtagttg cggcccacgc aatagatgcg
     3661 acgcaccgcg aattgctcgc tggaccccac gaccggcacc gcgacgactg gtgcgggtgc
     3721 aaatacgtaa ctcatcccgt tctccgtttt cctcaattgc gccgcgcccg cgcggcgata
     3781 aagcatggag tgtaacgcgc agaaaagcgc ggtgcagccg atctgcacag gagcgcgtga
     3841 accggcggcg ctggtgcatc gtctcgctgg atcgctggat cgctggatcg ctggatcgct
     3901 ggatcacggg atcacgggat cactggacca acggatcacc ggcgtctcgg cgtctcggcg
     3961 tctcggcgtc tcggcgcgtt ggagagttgg agcgttggag cgttggagcg ttggagcgtt
     4021 ggagcgttgg agcattggag cattggagca ttggagcgtt ggagcgtcgg aacgttggag
     4081 cgttggagcg tcgaatcgcc agatcgctgg agggtcggac cgtgggaccg ccatagatgc
     4141 gatactcaca ccgaatagca tcctttgaat accgtggccg actgccccag aagcggcgcg
     4201 ccccccgtcc ccactgcgcc cgatgactga ctccgccgac accgccaatc ctttcccctg
     4261 cgcccgttat atcaaggaaa tcggccgcgg cccgaacggc gcccgcgcgc tgaccgccga
     4321 agatacgcgc gcgctctaca ccgcgatgct cgagggccgc gtgtccgatc tcgaactcgg
     4381 cgcgatactg ctcgcgtatc gcgtgaaggg cgaaacagcc aatgaactcg ccgccatgct
     4441 ggccggcgcg catgcgtcgt tcgaaccgat tcatttgccg cacgcggcgt ttcggcccgt
     4501 gtcgataccc acgtacaacg gcgcgcgcaa acagccgaac ctggtgccac tgctcgcgct
     4561 actgctggca cgcgaggggg tgccggtgct ggtccatggc gtcactgaag atccgggccg
     4621 cgtgacgagc gccgaaatct tcacccattt gcagatcccg catgcgcaat cgcacgccga
     4681 tatcgaagac ggtctcgccg agcggcgtct tgcgttcgcg ccgatcgagg tcctcgcacc
     4741 gaagctcgcg cgcctccttt cgctgcgccg gcgaatgggc gtgcgcaatt caacgcatac
     4801 gctcgtgaaa atcctgcaac cgttcgcgcc tgcggggctg cggctcgtga attacaccca
     4861 tccgccgtac cgtgacagcc tcacacaact gttcaacacg cacccggacg ccgcagcagg
     4921 tggcgccctg ctcgcgcgcg gcaccgaggg cgaagcggtg gccgacacgc ggcgccaggt
     4981 gcaagtcgac tggctgcacg acggcatctg cgaaacgcgc gtgccgcacg agcgctcgtc
     5041 gtccgacgcg cccgaggtcg acttgcccga agcacgcgac gcccccacca cggccgcgtg
     5101 gatcagcgct gtgctacgcg gcgaagcgcc cgtgccgagc gcgatcgagc ggcaagtaca
     5161 gttgatcgcc gacgtcgcca aaacgcccct atgacaccgt atgacctgcc cggtgtgagt
     5221 gcaagactgc gcctgcggcc atgcgcgccc gcgccgaata tgacggttgc gggttgacac
     5281 gccacagtca gccgtcatac attcgaccca tgcgttccct tccgaacact ctccgaatta
     5341 cctccggccg cctcgcgcgg cgcctatcgc tacgcctggc gtaagtcaga cgtagcgctg
     5401 cgcgtggcag cctgatttag ttgcccaacg cgtggtcctc aaagcagttt cgcagttccc
     5461 gtttcacccc caaccgtagt tcttcacaac ctgtagtcgt ttgcattcgt ccgtttaccg
     5521 ttcggacgaa gcgcgatggt caaatgcacg ttgcacgggc ttcatccacg ttcgttctcg
     5581 ccgtcaccac gacgttgttt gttcgttcgc gcccgttcgt tgttgccgct agccaccggc
     5641 tggcgcgccg tgcatccgtg gcagcccgcc agccgctcga ggccacatcc cttcgcatac
     5701 gacacaacga acgaggctca tcatgttgcg caatcccgct gaaaaatacc gcccttttcc
     5761 cgctgtgcgc ctgaccggcc gcaaatggcc gagccgcacg atcagccagg cgccggtctg
     5821 gatgagcacc gatctgcgtg acgggaatca ggcactcatc gaaccgatga atgccgtgca
     5881 aaagctcgaa ttcttcgaga tgctggtcgc gatcggcgtc aaggagatcg aagtaggctt
     5941 cccgtcggcc tcgcaaaccg aattcgattt cgtccgcaaa ctgatcgaag aagagcgcat
     6001 tcccgacgac gtgacgattg cagtgttcgt gccctcacgc gcggaactga ttgcgcgtac
     6061 cttcgaatcg ctggagggcg cgccgcgcgc catcgtgcac ctgtacaacg cgatctgccc
     6121 gtcgtttcgc aagatcgttt tcaaccagtc gaaggacgaa atcaaggcgc tggccgtgga
     6181 aggcacacgc atcatcaagg agcacgctca ggcgcggccc ggtacgcgct ggacgttcca
     6241 gtattcgccc gaaaccttca acacgaccga gcttccgtat gcgcgcgaag tgtgcgacgc
     6301 ggtggcgcag acgtggcggc ccacgcgcaa tcacaagatg atcgtcaact tgcccgcgag
     6361 cgtcgaggcc gccacaccca acgtgttcgc cgaccagatc gaatggatgg accgcaatct
     6421 cagctatcgc gacagcatcg tgctgtcggt gcatccgcat aacgatcgcg gcacggcggt
     6481 ggccgcagcg gaactggcgc tgctggcggg cgcggatcgc gtcgaaggat gtctgttcgg
     6541 caacggcgag cgcaccggca atgtggacct cgtcacggtg gcgatgaatc ttcacacgca
     6601 gggggttgat ccgggcctcg atttctccca tatcgatgcg gtgcagcgcg tggtcgagcg
     6661 ctgcaaccgg attccggtgc atccgcggca tccgtatgcg ggcgagacgg caaagcaacc
     6721 cgcgtggagc gacgaggacc tgcagcgcgt agctgcttga gaagttctac agcgcgtggc
     6781 tgcctggaga ggatcagttg cacgattagc actgcaatcg cctacttgtg ccgtccaagc
     6841 gctcacaaag cactgataag gcgcttaccc agcgcaactg gcgaaacaga gtgagctcaa
     6901 aaggctcact cgatcgcgca acgcgtcgcc tgccaggcga tcagacgcca ttgcccgtct
     6961 tcctgcgtat gaatcgccgt gtacgcgatc ggaaacagca gcgccccatt gttcgcttcc
     7021 atttcgatca gcgcgcgccc ggtgacgacg caagtctcac gccccaccgg cagcacctct
     7081 tgcgactgca cctcgatctg ccgatagcgg cggcgcccag cggtaatacc gtcgatgaac
     7141 tgccgcttcg tttcacgctt gccgttcgtg tggacatagc tcacgttgtc cgcgagcagc
     7201 gtatcgagcg actgcccgtc gccgtcgacc atcgcacgga agcgctctcg ctcgagcccg
     7261 cgaatcgctt cgattatctt tgccgccatt gctcagcccc tcgcaccgac gagccgaacg
     7321 tgaccacggt cgtcagaagt tatattgcaa atatagctgg tggaaattta taccaggatt
     7381 gggctctttg ataccgccgt tggaaatatg ctggaagcga tagcccacct gatactgctg
     7441 gtggctgccg agttgcacgc ccacacccgc catgggcgtg aactggaacg ccgtggagta
     7501 ggtgtagtcg gtcgagaccg tgggacttgt caacaggcgc acacccgccc ccgcttcgat
     7561 aaatggacgg accgcgcccg ttccgttgat gaagcggatg atcggcgtca cgccgaattc
     7621 cccgatgttt tggtggacgt tcgcctcgcc ggtgtgccac aaggccgcat gcgcctcgcc
     7681 gatcagcgca aagtgccagt cgccgatctg cccccaagtc agattcggat cccagacgaa
     7741 gccgagatcg tattttttga agtgatggtc accggtaccg cccccaaact ggacgccgaa
     7801 ctggtcggcc gcggccatcc cggaagcgct aaccagcaca gccgtgcagg tcccttttag
     7861 agccagacca cgcaaagcgt cttttttgtt attcatcgaa cacccgagct agccgaacgt
     7921 tgattacctt ctcactgttg cgcctatttt aatgacattc ggaaattcgt ggatcatctg
     7981 aagaaatctt aatttccgaa attagtcacg ctgatttttt aaaaaatcgg tcacttatat
     8041 tattgaaggc aaatccttag gtaacttcta cggtgcgcgc tcgtcggctg caaccggcat
     8101 tcgatagcta tcagaaccta aatttcaata ggaatttgcg gaacttggat gcccctatgg
     8161 cttctaagta ttagcactcg gcaaatcaga gtgctaacat ccaacgctgg agtcgtttac
     8221 ctgaatacgc ttttgtctga ttccaaagga gtttcctacg tgagccatgc aatgaccctt
     8281 ccgagtactc tgagcccgtc gtcggctaag gccgcttcgg caggtgcgct ggcgctctcg
     8341 cattcctcgc tgctgcccgg ccaactgggc aatatcgacg cctacatcca ggccgtaaat
     8401 cggattccga tgctgacgcc ggcggaagaa cgccagttcg ccaccgaatt tcgcgagcag
     8461 gacaacctcg agtccgcgcg ccgtctcgtc ctgtcgcacc tgcggctggt cgtatcgatc
     8521 gcgcgcaatt atctgggtta tggactgccg catgccgacc tgatccagga aggcaatatc
     8581 ggcctgatga aggccgtgaa gcgcttcgac ccggagcaga acgtgcgcct cgtgtcgtac
     8641 gccatgcact ggatcaaggc tgagatccac gaatacattc tgcgtaactg gcggatggtg
     8701 aaggttgcca ccaccaaagc gcagcgcaag ctgtttttca acctgcgcag tcacaagcaa
     8761 ggcttgggcg cgttcacgcc ggacgagatc gaaggtctcg ccaaagagct gaatgtcaag
     8821 cgcgaggaag tttccgagat ggaaacgcgc ttgtccggcg gcgatattgc gctggaagat
     8881 caggtcgaag acggcgaaga gtcgtacgca ccaatcgctt accttgccga ctcgcatagc
     8941 gagccgactg ccgtgctagc ttcgcgtcag cgcgacaagc tgcagagcga cggtatcgcg
     9001 agtgcgctgg atgcactgga cgcccgcagc cgccgcatca tcgaggcacg ctggctgaag
     9061 gtggaagacg acggttcggg cggttcgacg ctgcacgaac tggccgacga attcggcgtg
     9121 tcggccgaac gtattcgcca gatcgaagcg agcgcaatga agaaaatgcg cggcgcattg
     9181 accgagtatg cgtaacactt agaagctttg gtcgcgccgg cattcgcccg gcgcgcgctg
     9241 cttagtcgca gagccctgcc ctcgcaagag ggcagggttt ttttgtgccc actgaacgac
     9301 acatacgccc aatcgcagac ccacgcggcg gccgctcaag aacattctcg ccaaacacgc
     9361 gtttcttgac gtatcacaaa ccccgtagca atactatcca aacggggcga ggctcgcgac
     9421 ccaatgccgc agcgctgaac tcacgcccgc aaggtcactg ctcctagaat cgggatgcac
     9481 tcgccagacc gctcaggcgg cctggatcca gccggattta ccggtgtgaa gtcgccttcg
     9541 accgatcatg accatagccc tgaatcgcca tagcaaccct cgcccgagcc tgcgcaaacg
     9601 gctcgccgcc tgggtgcgcg gtccgacgct ggcgcgggat atcgccatcg tccttgccat
     9661 caaattcgca ctgctgatgg cgctcaagta caccttcttc aatcatccgc aggcggagca
     9721 tatgtcgctg ccgcctgagc aagtcgcgca ggcgctgctg tccacgcctg cctcgcatcc
     9781 gtcccaaggt gatcaacatg cccgttagcg aagtcgtaga actgtcgcgc ctccagttcg
     9841 ccatcacggc gctttatcac tttctgttcg tgccgctcac gctcggtttg tcctggctgc
     9901 tggtcatcat ggaatccgtc tatgtgatga ccggcaaaca gatctacaag gacatgaccc
     9961 agttctgggg caagctgttc ggcatcaact ttgcgatggg ggtcaccacc ggtctcacgc
    10021 tggaattcca gttcggcacc aactggtcgt actactcgca ctatgtcggc gacattttcg
    10081 gcgtgccact ggccgtggaa ggtctgatgg cgttcttcct cgagtcgacc ttcgtcggcc
    10141 tgttcttctt cggatggaac cggctctcga aagtgcaaca cgtgatcgtc acgttcctcg
    10201 tcgcactcgg ctcgaacctg tcggcgctgt ggattctggt ggccaacggc tggatgaaca
    10261 atccggttgg cgccaccttc aactatcaga ccatgcgcat ggaactcgac agcatcttct
    10321 ctgtgctgtt caatccggtc gcgcaggtga agtttgtgca taccgtatcg gccggttatg
    10381 tgaccgcctc gatgttcgtg ctcggcgtgt cgtcgtggta tctgttgaag cgtcgcgaca
    10441 ccgaattcgc cctgcgatcg ttcgcgatcg ctgcgggctt cggtctggca tcaacgttgt
    10501 gtgtgatcgt gctcggcgac gaatccggct accgcaccgg cgaagttcag caggtcaagc
    10561 tggccgcgat cgaatccgaa tgggaaacgg cgcccgcacc cgcaccgttc acgatcatcg
    10621 gcattccgaa ccagaaggaa gaacgcaccg actatgcgat ccgcattccg tatgcgctcg
    10681 gtctcattgc cacgcgttcg atcgacgaac cggtggtcgg cttgaaagac ctgatggcac
    10741 agaacgaagt gcgcatcaag aacggcatgc tcgcctacgc cgcactgcaa cagctgaagc
    10801 taggtgacgc cacggacgaa gccaaagccg ccttcgacgc ccacaaggcg gacctcggct
    10861 acggcctgat gctcaagcag ttcaccccga acgtcacgga tgcgacgccg gatcagatcg
    10921 tgcaggccgc caagaagacg attccgccag tggcgcccgt gttcttctcg ttccgcctga
    10981 tggtcggcct cggcatcctg ttccttgcaa ccttcgtgct ggcgttctgg ttctgtgcgc
    11041 agcgctccct gctgctggag aagcgccgct ggttcctgcg ttgggccgtg tgggcgattc
    11101 cgttgccgtg gctcgcggct gaattcggct ggatcgttgc tgaagtgggc cgtcaaccgt
    11161 ggaccatcgc cggcattctg ccgaccgcat tgtccgcctc gagccttacg ccaagcgatc
    11221 tgtacctgag cattgccggc ttcgtcgtgt tctacacggt gctgttcatc atcgaaatca
    11281 tgctgatgtt caagtacgcg cgactcgggc cttcgtcgct gcacaccggg cgctatcacc
    11341 acgaacagcc gctgaatcag ccgctgccga ccaacacggc cgcttgattc gccgccattc
    11401 tgttccaagg gaaagatcat catggattac gcaaccctca aactgatctg gtgggcgttg
    11461 atcggcgtac tgctgatcgg cttcgcgctt accgacggct tcgacatggg cgcggcgatc
    11521 ctgctgccgt tcatcgccag aaccgacggg gagcggcgca tcgtcgtcaa cacggtcggc
    11581 gcgacgtggg aaggcaatca ggtgtggctg atcacggccg gcggcgcaat gttcgccgcg
    11641 tggccgctgg tctatgccgc gtcgttctcc ggcttctact ttgcgatgct gctggtgctg
    11701 ttctcgctat tcttccggcc ggtcggcttc gactaccgcg gcaagcgtga agacccgcgc
    11761 tggcgcagcg catgggactg ggcgctgttt gtcggcggct tcgtaccggc gctggtgttc
    11821 ggcgtggcgt tcggcaacct gctgcaaggc gtgccgttct cattcgacac cgatctgcgc
    11881 gtcacatatc acggtagttt cttcgcgctg ctcaacccgt ttgccgtggt gtgcgggctc
    11941 gtgagcgtgt cgatgctggc cgcgcatgga gccgccttcg tgaagatgaa agcggacgac
    12001 atcgtcgccg agcgtgcctc gctggcgctg cggatcgctt cgcttgcggc cgtggtgctg
    12061 tttctgcttg ccggcgcgct gatcgccacg atgatcggcg gctatcagat cattgacgcc
    12121 ccgccgctcg atacggttgc caacccgctg ctgaagaacg taatcggcgc acctggcctg
    12181 tggctcacca actacgcaaa ctatccgtgg atggtggctg cgccggcggc gggtgtgatc
    12241 ggtggcgtac tcgcgttgtt gctcgcgcgc tcgcgttttg aaaagagcgc cttcctgtcg
    12301 acctcgctga tgatcatcgg cgtgattctg acggcggcct tctcgatgtt cccgttcatc
    12361 atgccctcct cactcgacgg ccgcagcagc ctgaccgtgt gggattcgac ctcgagccgc
    12421 atgacgctgc agatcatgct gatcgccgta atcattttcc tgccgatcgt cctgatctac
    12481 acgagctggg tgtatcgcgt gatgcgaggc aaggtgaccg ccgcggcgct cgaagaaaat
    12541 catcattcga tgtattgatc ggcaatacga ttaacaggag tttgtgatgt ggtatttcag
    12601 ttggcttctc ggcat
//
LOCUS       DATASH010000095         2787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4364792,
            whole genome shotgun sequence.
ACCESSION   DATASH010000095 DATASH010000000
VERSION     DATASH010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4364792"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /gene="glcE"
                     /locus_tag="VHV99_02910"
     CDS             <1..672
                     /gene="glcE"
                     /locus_tag="VHV99_02910"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HEX3634433.1"
                     /translation="VKNVAGYDVSRLLAGSLGTLGLILEVSVKVLPKPVAEATLKFEM
                     NDTDAVRKLNEWSGHPLPLTGSAWRDRTLALRLGGAEAAVKAARTSLGGEVVDAVEAE
                     RFWCGLREQSDPFFAVIPPKSALWRLSLPSIAEPMHLPGPHLMEWGGAQRWWITDADP
                     QTVRISAKQAGGHATIFRSSAAYDRNAGVFTPLPAPLMKIHRGLKAAFDPASIFNRGR
                     LYPDF"
     gene            689..1915
                     /gene="glcF"
                     /locus_tag="VHV99_02915"
     CDS             689..1915
                     /gene="glcF"
                     /locus_tag="VHV99_02915"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HEX3634434.1"
                     /translation="MQTNLADFMRDTADGEEAEAILRKCVHCGFCTATCPTYQILGDE
                     LDGPRGRIYLIKQMLEGAPVTRSTQLHLDRCLTCRNCETTCPSGVQYGRLVEIGRKVV
                     EEKVERPFGQRVQRKLLANFLPNSKLFTPAMKLGQQFRPLLPKALRTKIPVAQRPLEW
                     PTSTHERTMLMLAGCVQPAMMPNVNIATARVFDALGIQILTAPDAGCCGAIRLHLGYN
                     DEALDDVRSNIDAWWPYVENGVEAIVMNASGCGATVKEYAHLLRNDPLYAEKAARIVD
                     LTRDLSEILPQYEDELSAMTRRRAVHTVAFHPPCTLQHGQQIRGHVENLLTALGIEVH
                     LPTDSHLCCGSAGTYSVLQPKLSYALRDQKLDTLEKLEPQMIVSANVGCIAHLQSGTS
                     TPVVHWIQLVEHLLYG"
     gene            1997..2701
                     /locus_tag="VHV99_02920"
     CDS             1997..2701
                     /locus_tag="VHV99_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006999235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEX3634435.1"
                     /translation="MSSSHVTRHLDEVRQRIAKAAADASRDASSVTLLAVSKTFPAED
                     VRAAFNAGQRAFGENYVQEAVAKITGLADLRSEIEWHFIGPLQSNKTKVVAENFDWVH
                     SVDRLKIAERLNEQRPEDLPRLSVCLQINVSGEDSKSGVAPGDALALAHQIAVLPRLH
                     LRGLMAIPEPAGTLDEQRAPHRRLREIMDTLRSDGLELDTLSMGMSADIEAAVLEGAT
                     VVRIGTAIFGARDYSH"
BASE COUNT          540 a          845 c          878 g          524 t
ORIGIN      
        1 gtgaagaacg tggcgggtta cgacgtctcg cggctgctgg cggggtctct cggcacgctc
       61 gggctgatcc tggaggtatc ggtgaaagtg ctgccgaaac ccgtcgccga agccacgctc
      121 aagttcgaga tgaacgatac cgacgccgtg cgcaagctca acgagtggag cggccatccg
      181 ctgccgctga ctggcagcgc gtggcgcgac cgcacgctcg cgttgcgcct tggcggcgcg
      241 gaagcggcgg tgaaagcggc gcggacatcg ttgggcggcg aagtggtgga cgccgtggaa
      301 gccgagcgtt tctggtgcgg tttgcgcgag caatccgatc cgttcttcgc ggtaataccg
      361 ccgaagtccg cgttgtggcg gctgtcgctg ccatcgattg ccgagccgat gcatctcccc
      421 ggccctcact tgatggaatg gggcggcgcg caacgctggt ggatcaccga cgccgatccg
      481 caaaccgtgc gcatcagcgc caagcaggcg ggaggccacg cgaccatctt ccgctcgagc
      541 gcggcgtatg accgcaacgc gggcgtcttc acgcccctgc ccgccccgct gatgaagatt
      601 catcgcggcc tgaaagctgc attcgatccc gcgagcattt tcaatcgcgg ccgtctgtac
      661 cccgactttt gaaccgaaga cacacgcaat gcaaaccaac ctcgccgact tcatgcgcga
      721 caccgccgat ggcgaagaag ccgaggccat cttgcgcaag tgcgtgcatt gcggattctg
      781 cacggcgacg tgcccgacct atcagatact gggcgatgaa ctcgacggcc cgcgtggccg
      841 gatctatctg atcaagcaga tgctggaagg cgcacccgtc acacgcagca cgcaactgca
      901 tctggatcgc tgcctcacgt gccgtaattg cgagaccacg tgcccgtcgg gcgtgcagta
      961 tggacgactg gtggaaattg gccggaaggt cgtggaagag aaagtggagc ggccgttcgg
     1021 ccagcgcgtt caacgcaaac tgctcgccaa cttcctgccg aacagcaagc ttttcacgcc
     1081 cgcgatgaaa ctcggccagc agttccggcc gctgttgccc aaagccttgc ggaccaagat
     1141 tcccgtcgcg caacggccgc tcgaatggcc aaccagcacg catgagcgca cgatgctgat
     1201 gctcgccggc tgcgttcagc ccgccatgat gccgaacgtc aacatagcga ctgcacgagt
     1261 attcgacgca ttaggcatcc agatcctgac tgcgcccgac gcgggttgtt gcggcgcgat
     1321 ccggctgcat ctcggctaca acgacgaagc gctggacgac gtgcgcagca acatcgacgc
     1381 gtggtggccg tatgtggaaa acggcgtgga ggcgatcgtg atgaacgcgt ccggttgcgg
     1441 cgcgacggtc aaggaatacg cgcacttgct gcgcaatgat ccgctctacg cggagaaggc
     1501 cgcgcggatc gtcgatctga cccgtgacct gtcggaaatc ctgccgcaat acgaagacga
     1561 actgagcgcg atgacaaggc ggcgcgcggt ccataccgtc gcgttccatc cgccctgcac
     1621 gctgcagcac ggccagcaga ttcgcggtca cgtggagaac ttgctgacgg cgctgggcat
     1681 agaagtgcat cttcccaccg atagccattt gtgctgcggc tcggccggca cgtactcggt
     1741 gctgcagccg aaactttcat acgcattgcg ggaccagaag ctcgatacgc tcgaaaagct
     1801 cgagccgcag atgatcgtat cggcgaatgt gggttgtatt gcgcatttgc agagcggcac
     1861 gtcaacgccc gtcgtgcatt ggattcaact ggtagaacat ctgctgtatg gctaacgctg
     1921 gcgggcgccg gctgattcga ggcgccgcat ccgtataatc ggacctctgc ttactcgatt
     1981 tgcggcgtcc gaatccatgt cctcctctca tgtaacccgt cacctcgacg aagtccgtca
     2041 gcgcattgcc aaagcggcgg cggatgcttc gcgcgatgcg tcttcggtca cgttgctggc
     2101 ggtatcgaag acctttcctg ccgaggacgt tcgtgcggcc ttcaacgccg gacaacgcgc
     2161 gttcggcgaa aactacgtgc aggaagcggt cgcgaaaata accgggctgg cagatttgcg
     2221 tagcgagatc gagtggcatt tcatcggacc gttgcagtcc aacaaaacca aggtcgtggc
     2281 ggaaaatttc gactgggtgc attcggtcga ccggctcaag attgccgagc gtctcaatga
     2341 acagcggccg gaggatttgc cgcggctcag cgtgtgtttg cagatcaacg tgagcggcga
     2401 agactcgaaa agcggcgtcg cgccgggcga cgcattggcg ctcgcgcatc agattgcggt
     2461 gctgcctcgc ttgcacctgc gcggcttgat ggccattccc gaaccggccg gcacactcga
     2521 tgaacaacgc gcgccgcatc ggcgtctgcg cgaaatcatg gacaccttac gctcggatgg
     2581 ccttgaactc gacacgcttt ccatggggat gtcggcggat attgaagccg ctgttcttga
     2641 aggcgcgacc gtggttcgta tcggcacggc tatcttcggc gcgcgtgatt actctcactg
     2701 actttcgact tgaactcact atgaaaattg cgtttattgg tggcggcaac atggcgtcgg
     2761 cgcttgtcgg cgggttgatc aagcgtg
//
LOCUS       DATASH010000096         2049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_804016,
            whole genome shotgun sequence.
ACCESSION   DATASH010000096 DATASH010000000
VERSION     DATASH010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804016"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /gene="mraY"
                     /locus_tag="VHV99_02925"
     CDS             complement(<1..996)
                     /gene="mraY"
                     /locus_tag="VHV99_02925"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX3634436.1"
                     /translation="MLLALAQWLQNDASFLRMFSYLTFRAVMATITALLIGLVCGPAV
                     IRKLTAMKVGQAVRKDGPQTHLVKSGTPTMGGVLILLGIAVATLLWADLTNRFIWIVI
                     LVTFGFGVIGWVDDYRKVVYKDPRGMSSREKYFWQSVIGLFAAVYLAFSVSEASNVRV
                     FDLFMAWVRSGLSMGLPARADLMLPFVKSISYPLGVWGFIVLTYLVIVGASNAVNLTD
                     GLDGLVIMPVVLVGASLGVFAYVMGSSVYSKYLLFPHIAGAGELLIFCSAMGGAGLAF
                     LWYNTHPAQVFMGDVGALALGGALGTIAVIVRQEIVLFVMGGIFVVETLSVMLQVS"
     gene            complement(1014..>2049)
                     /locus_tag="VHV99_02930"
     CDS             complement(1014..>2049)
                     /locus_tag="VHV99_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX3634437.1"
                     /translation="AAVGADACLATAGNFNNDIGLPLTLFRLHTAHQLAVVELGMNHP
                     GETSLLAKIAEPTVAVVNNAQREHQEFMATVEAVALEHASVIHALTPEGVAVFPADDA
                     YASIWRVAATGNRIIDFALNDDERNTEAAVKGTFAGNRLSIDTPEGHVDVTLQVLGNH
                     NAHNALAATAAALAAGVSLDAIKRGLESFGAVKGRLQVKLAALGTLAGATVIDDTYNA
                     NPDSMRAAIDVLASRESPRVLVMGDMGEVGDSGPAFHREIGAYAKERGIDALYAMGDA
                     ARDACTAYGAGAHHVADVGTLVAQLQQAGFGAAATLLVKGSRFMQMERVVDAVTSPQP
                     NATGNAPAAH"
BASE COUNT          394 a          677 c          641 g          337 t
ORIGIN      
        1 cgagacctgg agcatcacgg acagcgtctc gacgacaaag atcccgccca tcacgaacag
       61 cacgatctcc tggcggacga tcaccgcgat cgtgccgagc gcgccgccga gcgcgagcgc
      121 accgacatcg cccatgaaca cctgcgcggg gtgcgtgttg taccagagga aggcgaggcc
      181 cgcgccgccc atcgccgaac agaagatcag cagttcgccc gcgccggcga tatgcggaaa
      241 caacaaatac ttcgagtaga ccgagctgcc catcacatac gcgaacacac cgagcgacgc
      301 tccgaccagc acgaccggca tgatcacgag accgtcgagg ccgtcggtga ggttgaccgc
      361 gttgctcgcg ccgacgatca ccaggtacgt cagcacgatg aagccccaca cgccgagcgg
      421 atagctgatc gatttgacga acggcagcat caggtcggcg cgcgccggca ggcccatcga
      481 caaaccactg cgcacccagg ccataaacag atcgaacacg cgcacgttgc tcgcttcgga
      541 gacgctgaac gcgagataca ccgctgcgaa caaaccgatc accgactgcc agaaatactt
      601 ttcacgcgac gacatgccgc gcgggtcctt gtagaccacc ttgcggtaat cgtcgaccca
      661 gccgatcacg ccgaaaccga acgtgaccag aatcacgatc cagatgaaac gattggtcaa
      721 atcggcccac aacagcgtcg ccacggcgat gccgagcaga atcagcacgc cccccatggt
      781 cggcgtaccg gacttgacga ggtgcgtttg cggaccgtct ttacgaacgg cctggccgac
      841 cttcatcgcg gtcagcttgc ggatcaccgc cgggccacag acgagcccga tcagcagcgc
      901 ggtgatggtg gccatcaccg cacggaaagt cagataactg aacatgcgca agaagcttgc
      961 gtcattctgc agccattgcg ccagcgccag gagcatgctt cggtccttct atttcaatgt
     1021 gcagcgggcg cgttgcccgt tgcgttgggt tgtggactcg ttacggcgtc caccacgcgc
     1081 tccatttgca tgaagcgcga gcctttcacg agaagcgttg cagcagcgcc gaaaccggcc
     1141 tgctgcaatt gcgcgaccag cgtgccgaca tcggctacgt gatgcgcgcc cgcgccgtac
     1201 gcggtgcagg cgtcgcgcgc ggcgtcgccc atggcgtaca gcgcgtcgat accgcgttcc
     1261 ttggcgtagg caccgatttc acggtgaaac gccgggccgc tgtcgccgac ttcacccatg
     1321 tcgcccatca ccagcacgcg cggcgattcg cgcgacgcga gcacgtcgat cgcagcgcgc
     1381 atcgaatcgg gattcgcgtt gtaggtgtcg tcgatcacgg tcgcgccggc gagcgtgccg
     1441 agcgcagcaa gcttgacctg caggcggcct ttcaccgcgc cgaacgattc gaggccgcgc
     1501 ttgatcgcgt cgagcgacac gccggcggcg agcgccgctg cagtggcggc caacgcgttg
     1561 tgcgcgttgt ggttgccgag cacttgcagt gtcacgtcga cgtggccttc cggcgtgtcg
     1621 atgctcagac ggttgccggc gaacgtgccc ttgactgcgg cttcggtgtt acgttcatcg
     1681 tcgttcaggg cgaagtcgat gatgcggttg cccgtggccg cgacgcgcca gatgctcgcg
     1741 tacgcatcgt ccgccggaaa cacggcgacg ccttccggcg tgagcgcgtg aatcacgctc
     1801 gcatgttcga gcgcgaccgc ttcgaccgtc gccatgaatt cctgatgctc gcgctgggcg
     1861 ttgttcacca ccgcgaccgt cggctcggcg attttcgcga gcagcgaggt ctcgcccgga
     1921 tggttcatgc cgagttcgac caccgctagt tgatgcgcgg tatgcagacg gaacagcgtg
     1981 agcggcaagc cgatgtcgtt gttgaagttg ccggcggtgg caaggcatgc gtccgcgccg
     2041 accgcggcg
//
LOCUS       DATASH010000097         7981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1398428,
            whole genome shotgun sequence.
ACCESSION   DATASH010000097 DATASH010000000
VERSION     DATASH010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7981
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1398428"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /gene="tssK"
                     /locus_tag="VHV99_02935"
     CDS             <1..420
                     /gene="tssK"
                     /locus_tag="VHV99_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004533421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HEX3634438.1"
                     /translation="RRALSTVLEQNAIALELQERAHRVWVAQIPSVELLHHAGFVLAV
                     HADLPAETVRTRYPAQVKIGPVERLAELVHLQLPGIAMRSLPVAPRQIPYHAGYHYFE
                     LDAGGELWKQLEQSGGLALHVAGDLPGLTMECWAIRG"
     gene            452..1780
                     /gene="tssL"
                     /locus_tag="VHV99_02940"
     CDS             452..1780
                     /gene="tssL"
                     /locus_tag="VHV99_02940"
                     /inference="COORDINATES: protein motif:HMM:NF038228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, long
                     form"
                     /protein_id="HEX3634439.1"
                     /translation="MSTIAAAPAQRTGTTPSSPAVGAGAASRLESRPESGFETPFAPS
                     VPQPSAYASNPLVAAALPLFDALAQIRLNATVTPECLRAFLVAEIRRFQTRAQQAAVS
                     IETIVGARYCLCTALDEAAALAPGGGSGAWSAHSLLVAFHNETWGGEKFFHLLARLSA
                     QPREHRDLIELQFFCLALGFQGRYRVIQNGAAQLETLTRRLHKLLHETAGGYALPLSP
                     HWCAADRTPARALRRGMPLWMWLAGAALLGGASFIAFLLATNAYGDRTYAAINAIRLP
                     EVTSQRALDPVPHVAALLEADVQSGALSVRDEGDRSVIALRGDGLFGSGSAVASEAAL
                     PVLSHLAASLDRVPGDILITGHTDNQPIHDQRFASNLALSLARAEAVRQWLISAGFDP
                     SRPVKVAGRGDQQPLDSNATAAGRARNRRVEIAVYPASAARQELVPKADR"
     gene            1777..4359
                     /gene="tssM"
                     /locus_tag="VHV99_02945"
     CDS             1777..4359
                     /gene="tssM"
                     /locus_tag="VHV99_02945"
                     /inference="COORDINATES: protein motif:HMM:TIGR03348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HEX3634440.1"
                     /translation="MNSLKRIAGWLAGLLRQLGWASLAWLALLISLIWIAGPMLAFGD
                     TRPLEPERSRYALIALVLLAWTWRQARRTRESRLPEPLRRASSVSRTDPADGAQHQRL
                     DDLRARFDHARYVLRHATVPAGRLATWFDRLTGRHVYRLPWYLIVGSGAAGKTAALLN
                     GGLELSIAEQAARAASRRTEPTHACDWWFSNDAVLVDTPGAYLDAADAADARNAEWRE
                     LLALLKRHRPRQPLNGVVLAVGVDALLTLDEAGRAAYATRLRKPLQLMQSTFGMRVPV
                     YLCITRMDRLDGFRAYFSGLNRDGRAQAWGAALESGDSVLTETCQRAFQALQQRLTDG
                     LRDVLIVEPDPVSRARAFLFPQQFASIEEPLVDFCEALFRPSPLETNLLPRGVYFTSA
                     LQGGPGIDRVLPATRQQMGIADSLPAAAEPPGQHSYFLKQWLREAVFADAGFAGSSRG
                     SQRRRFVVHTMIAAIAGIGLVGLLAGWAISYAHNRGYLDEVSMHVGALNRLSAQPVTL
                     TAGDLQPLSPLLDTLRALPRSENVDTDAPPGWHYGMGLYQGARIREASDALYRRALDE
                     KLLPQAAARIERMLADAPADDADYSYEALKAYLMLYDASRYDAGFLTAWLTLDSRGAL
                     PSDLTQDERARLEAHLANLFASRVVTSPFAFNAPLVDAVRARLAHESPAQRANHQLSR
                     ELLRTLRNAPVTVAGAGGPQAALVLTRRSGKPLTDGIASLYTYHGYWDGFEPRVAAAA
                     ARLQSEDPWVLGIHPTPATDNARLALEIRRMFLNDYIQAWDAYLNDLSIVDSQPLAQS
                     MQIARTLSAPDSPLKQFLQAAADETTLLRARAGSEQPAAGRTAKTHWRSERIAERDVR
                     PRFA"
     gene            4361..5365
                     /locus_tag="VHV99_02950"
     CDS             4361..5365
                     /locus_tag="VHV99_02950"
                     /inference="COORDINATES: protein motif:HMM:NF018454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion IcmF C-terminal
                     domain-containing protein"
                     /protein_id="HEX3634441.1"
                     /translation="MSPPIVDDQPEGIVDSHFEPLRRLVTAGGASAANGTPLEGNLRV
                     LDELYSYLTAADAALASGSPPPQTDVFNKLQADAGRLPAPLRKIVGDLSQASALQVSG
                     GARRNVAQDAQGSLGPLCRQVIAGRYPFARGSNRDVALDDFTRLFASGGLMDSFFQKN
                     LASQVDVRDGRWAFRRDASGKMIADSHLLSAFQNAETIRNVFFATGAATPSLQIELTP
                     LELEPGITQYALNIDGQTMRYAHGPPLPMAVKWPGERGVGLVSLQISTQTGSDGVQTL
                     GPWALYRLFDKARITPGAAPESFVATFDFSGRKLALRVSANSSYNPFQLPQMKAFSCP
                     "
     gene            5393..6523
                     /locus_tag="VHV99_02955"
     CDS             5393..6523
                     /locus_tag="VHV99_02955"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX3634442.1"
                     /translation="MLQPSLQLTILASHGKALASGPCASFVPQGGNIGRAADSTLRLP
                     DDDTVAERHAVIRAQYGAWCLLNIGGRAALTVNGKPIAAGQESRLQSGDIVNIGSYVL
                     QATETLPPAWDFQISRPSGAISQDPNLPRPDIANGKSASFATPPMEPLVFGRLDGQSA
                     SEPPSAELDGLLDTPVDPLALFGAPRHGWDNPTEANSSDLFADLAGSRAASGFEATPT
                     DGLHGFALRDDAPEHGAPLRLRIANAPTPGPSDSTAAPIATAMAATQPAFGESSPATE
                     RAAQVAVPQYGEHSQVKAALRHGGLIRVSCANTPVSLNDAPQEPQEPHPGNGDAATSA
                     LLALSEAFLDGAGIHPDRHVEAGLTPEFMRALGAIARALRAR"
     gene            6619..7917
                     /locus_tag="VHV99_02960"
     CDS             6619..7917
                     /locus_tag="VHV99_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEX3634443.1"
                     /translation="MEQLPLWAQICAVFLLLICSSFFSISETAMMAINRHRLKHLANQ
                     NALGAKTTQGLLAHTDQLLSVVLIGNNLFNTIIPVLTTSIALHTFGRNNVVLSIATGI
                     VAFLIIVFAEITPKIVGATFPEKIALPASLLIAPMMRVGKPLVWFVNLFANTILRVLH
                     INTKGAHDQRLSTEELRTIVLESGSFMPTKHRSILLNLFDLENISVDDVMIPRRRIEA
                     LDFDAPFEQILHQLETCYHNKLIVYQGDIDRVLGVLHVRKTLAALHNQELERETLREL
                     LAEPYFVPTGTPVFQQLQYFQESRHRTALIVNEYGELQGLVTPEDIIEELIGEFTTTI
                     PRSANSRGGWNEKGECIVAGSMPLRELNRWLLLTMPTDGPKTLNGLILEILEDIPDGD
                     VCVQIGELKLEVMRSDDQAIRTVKLFRPPVRAKASKALRR"
BASE COUNT         1432 a         2730 c         2466 g         1353 t
ORIGIN      
        1 cgccgcgcac tgtcgaccgt tctcgaacag aacgccatcg cactcgaatt gcaggagcgc
       61 gcgcaccgcg tctgggtggc acagatcccg agcgtcgagc ttctgcacca tgccggcttc
      121 gtgctcgcag tgcatgcgga tctaccggca gaaaccgtgc gcacgcggta tccggcgcaa
      181 gtgaagatcg gcccggtcga acggctcgcg gaactggtgc acctgcagtt gccgggcatc
      241 gccatgcgca gcttgccggt cgcgccgcgg cagatcccgt atcacgcggg ctatcactat
      301 ttcgaactcg acgccggcgg tgaactctgg aagcaactcg aacagtccgg cggcctcgcg
      361 ctgcacgtgg ccggcgacct gccgggcctg acgatggaat gctgggccat tcgcggctga
      421 tctcatgtat gcgaaagaaa ggcgaataaa catgtcaacc atcgcagcag cgcccgcgca
      481 gcgcacagga accacgccgt catcgccggc ggtcggggca ggagcggcgt cgcggctcga
      541 gtcacggcct gagtcaggat tcgagacgcc gttcgcgcca tccgtgccgc aaccctcggc
      601 ctacgcgtcc aatccactcg tcgccgccgc gctgccactg ttcgacgcgc ttgcgcagat
      661 ccgtctcaac gcgacagtga caccggagtg tctgcgcgca ttcctcgtcg cggaaatccg
      721 gcgttttcag acgcgcgccc agcaggcggc cgtgtcgatc gaaacgatcg tcggcgcgcg
      781 ctattgcctg tgcaccgcgc tcgacgaagc cgccgcactc gcgccagggg gcggcagcgg
      841 cgcatggtcc gcgcacagtc tgctcgtcgc gtttcataac gaaacgtggg gcggcgaaaa
      901 gttcttccac ctgctcgccc gtttgtcggc acagccgcgc gaacatcgcg acctcatcga
      961 gctgcagttt ttctgtctcg cactaggatt tcaaggacgc tatcgcgtga tccagaacgg
     1021 cgcggcacaa ctcgaaacgc tgacgcggcg cctgcacaag ttgctgcatg agacggcagg
     1081 cggctacgca ttgccgctat cgccgcattg gtgcgccgcc gaccgcacgc cggcacgcgc
     1141 gctccgccgc ggcatgccgc tgtggatgtg gctggcaggc gccgcgctgc tcggtggcgc
     1201 gagctttatc gcatttctgc ttgccacgaa tgcgtacggt gatcgcacct atgcggcgat
     1261 caatgcgatc cgcctgccgg aagtgacttc gcagcgcgcg ctcgaccccg tgccgcacgt
     1321 cgccgcgctg ctcgaggccg acgtgcagag cggcgcgctt agcgtgcgcg acgaaggcga
     1381 tcgcagcgtc atcgcgttgc gcggcgacgg actgttcggc tccggatccg ctgtcgccag
     1441 cgaggccgcg ttgccggtgc tcagccatct tgcggccagc ctcgatcgcg tgccgggaga
     1501 catcctgatc accggacaca ccgacaatca gccgatccac gaccagcgct tcgcctcgaa
     1561 tctcgcgctc tcgctggccc gcgccgaagc cgtcaggcaa tggctgatct cggccggttt
     1621 cgaccccagc cggcccgtga aagtcgccgg acgcggcgac cagcagccgc tcgacagcaa
     1681 cgcaacagcg gccggccgcg cgcgcaaccg gcgcgtcgag atcgcggtgt atcccgcctc
     1741 ggccgcgcgc caggagcttg tgccgaaggc ggaccgatga atagcctgaa acgcattgcc
     1801 ggatggctgg cgggcctgtt gcgccaactc ggctgggcaa gtttggcgtg gctcgcgctt
     1861 ctcatctcgc tgatctggat cgcgggcccg atgctggcgt tcggcgatac gcggccactg
     1921 gaaccagaac gatcccgcta tgcactgatc gcgctggtgc tgctcgcgtg gacgtggcgg
     1981 caggctcggc gcacgcgcga gtcacggctg ccggagccgt tgcgccgcgc atcttccgta
     2041 tcgcggaccg accccgccga cggcgcccag catcaacgcc tcgacgacct gcgcgcgcgc
     2101 ttcgatcacg cgcgctacgt gctgcggcac gccaccgtcc ctgccggccg gcttgccacg
     2161 tggttcgaca gactcactgg gcggcacgtg taccggttgc cgtggtatct gatcgtgggc
     2221 agcggcgcag ccggcaaaac ggccgcgctg ctcaatggcg ggctcgaact gtcgatcgcc
     2281 gagcaggccg cgcgcgccgc cagccggcgc acggagccga ctcacgcgtg cgattggtgg
     2341 ttcagcaacg atgccgttct cgtcgatacg cctggcgcct acctcgatgc ggccgatgcc
     2401 gccgacgcgc gcaacgcgga gtggcgtgaa ctgctggcgt tgctcaagcg ccatcgccca
     2461 cgccagccgc tcaatggcgt cgtcctcgca gtcggcgtgg acgccttgct gacactcgac
     2521 gaagccgggc gcgcggccta tgcaacgcgg ctgcgaaagc ctttgcaact gatgcagagc
     2581 acgttcggca tgcgtgtacc cgtctatctg tgcatcaccc ggatggatcg cctcgacggt
     2641 ttccgcgcct atttttccgg actgaatcgc gacggccggg cgcaggcgtg gggcgcggca
     2701 ttggaatccg gcgacagcgt gttgaccgag acctgccagc gcgcgtttca agcgttgcag
     2761 caacgcctga cggatggctt gcgcgacgtg ctgatcgtcg agcccgatcc ggttagccgg
     2821 gcgcgcgcct ttctgtttcc gcagcaattc gcgagcatcg aggagccgtt ggtcgacttc
     2881 tgcgaggcgc tgtttcgacc ctcgccgctt gaaaccaatc tgctgccgcg cggcgtctat
     2941 ttcacgagcg cgctacaagg cggcccgggt atcgatcgcg tgctgccggc gacccgccag
     3001 cagatgggca tcgccgattc actgcccgcg gccgccgagc cgccgggtca gcacagctat
     3061 tttctgaagc aatggctgcg cgaggccgta ttcgccgatg cgggattcgc gggttcaagc
     3121 cgcggctcgc aacggcggcg tttcgtcgtg cacacgatga tcgccgcaat cgccggtatc
     3181 gggctcgtcg ggctgctggc cggctgggcg atcagttacg cgcacaatcg aggctacctc
     3241 gatgaagtgt ccatgcacgt cggcgcattg aatcgactgt cagcgcaacc cgtcacgctg
     3301 acagccggcg acttgcagcc gttatcgccg ttgctcgata ccctgcgggc gttgccgcgt
     3361 agcgaaaatg tcgacaccga cgcgccgcct ggatggcatt acggtatggg gctttatcaa
     3421 ggcgccagga tccgcgaagc aagcgacgcg ctctaccgcc gcgcactcga cgaaaaactg
     3481 ttgccgcagg ccgctgcacg catcgagcgc atgctggccg acgcgccggc cgacgacgcc
     3541 gactattcgt acgaagcgct caaggcctat ctgatgcttt acgacgcgtc ccgctacgac
     3601 gccggctttc tcaccgcgtg gctgaccctc gactcccgcg gcgcgttgcc atccgacctg
     3661 acgcaggacg aacgcgcacg gctggaagcg cacctggcga acctgttcgc gtcgcgcgtc
     3721 gtcacgtcgc cgttcgcatt caacgcgccg ctcgtggacg cagtgcgcgc gcggctcgcg
     3781 cacgaatcgc ccgcgcaacg cgccaaccac cagctcagtc gcgaactgct gcgcacgctg
     3841 cgcaacgcgc ccgtcacggt ggccggcgcc ggtggcccgc aagcagccct cgtattgaca
     3901 cgccgaagcg gcaagccgtt gaccgacggc atagcgagcc tctacaccta tcacggctac
     3961 tgggatggtt tcgaaccgcg cgtcgccgcg gctgcagccc gcttgcaaag cgaagacccg
     4021 tgggtgctcg gcatccaccc gacgcccgcg accgacaacg cacgcctcgc cctggaaatc
     4081 cgccgcatgt tcctgaacga ctacatccag gcatgggacg catacctgaa cgatctaagc
     4141 atcgtggata gccaaccgct tgcgcaaagc atgcagatag cgcgcacctt gtccgcgccc
     4201 gattcgcccc tcaagcaatt cctgcaagcc gccgccgacg aaacaacctt gttgcgcgcc
     4261 cgcgccggca gcgagcagcc cgccgccggg cgcactgcaa aaacgcattg gcgaagcgaa
     4321 agaatcgctg agcgcgatgt tcggccacgc ttcgcctgag atgtcgccgc ccatcgtgga
     4381 cgaccagccg gagggcatcg tcgacagtca tttcgagccc ttgcggcgtc tggtgacagc
     4441 cggcggggcc agcgcggcaa acggcactcc gctggaaggc aatctgcgtg tgctcgacga
     4501 actgtacagc tatctcaccg ctgccgatgc cgccttggca agcggcagtc cgccgccgca
     4561 aaccgacgtc ttcaataaat tgcaagctga cgcgggacgt ctgccggcgc cgttgagaaa
     4621 gattgtcggc gacctgtcgc aggcgagcgc gctgcaggtc agcggcggtg cacggcgcaa
     4681 cgtcgcgcag gatgcgcaag gcagcctcgg cccactgtgc cggcaggtca tcgccgggcg
     4741 ctacccgttt gcacgcggct cgaaccgcga cgtcgcgctg gacgacttca cgcggttgtt
     4801 tgcctcaggc ggactgatgg acagcttctt ccagaagaat ctggcgtcgc aggtcgacgt
     4861 ccgcgacggc cgctgggcat tccggcgcga cgccagcggc aagatgatcg cggacagcca
     4921 tttgctcagc gctttccaga acgcggaaac gattcgcaac gtcttctttg ccaccggcgc
     4981 ggcgacaccg tcgctacaga tcgaactgac gccgcttgaa ctcgaaccgg gcatcacgca
     5041 atacgcgctg aatatcgacg ggcagacgat gcgctatgcg catgggccgc cactcccaat
     5101 ggcagtgaaa tggccgggcg agcggggcgt cggcctcgtc agcctgcaaa tcagcacgca
     5161 aaccggttcc gacggcgtgc agaccctagg tccgtgggcg ctctatcgac tattcgataa
     5221 ggcgcgcatc acgccgggcg ccgcgcccga aagctttgtc gccacctttg atttcagcgg
     5281 ccggaaactg gcgcttcgcg tgagcgcgaa cagtagttac aacccgttcc agctacctca
     5341 aatgaaagcg ttctcgtgtc cgtgagaatc gctcgctgca ggaaacatta cgatgctcca
     5401 gccgtctctt caactcacca ttctcgctag ccatggtaaa gcgcttgcca gcggcccatg
     5461 cgcaagcttc gtgccccagg gcggcaacat cggccgcgct gccgacagca cgctcaggct
     5521 gcctgacgac gataccgtcg cggagcgcca cgccgtcatc cgcgcgcaat acggcgcatg
     5581 gtgtcttctt aatatcggcg ggcgcgcggc gctgacggtg aatggcaagc cgatcgccgc
     5641 gggccaggag agccgtttgc agtccggcga tatcgtcaat atcggcagct atgtcttgca
     5701 ggcaaccgag acattgccgc cggcatggga ctttcagatc agcaggccat ccggcgccat
     5761 ctcacaggat ccgaacttgc ctcggccaga cattgcaaac ggcaaatcgg ccagctttgc
     5821 aacgccaccc atggaacccc tggtattcgg gcgccttgac ggccagtctg ctagcgagcc
     5881 gccgtccgcc gagctggacg ggctgctcga cacgccggtc gacccgcttg cgctatttgg
     5941 cgcgccaagg cacggttggg acaacccgac cgaagcgaat tcatcagact tgttcgccga
     6001 cctagcgggc agccgggccg ctagcggctt tgaagccact cccacagacg ggctgcacgg
     6061 tttcgcgctg cgcgacgacg cgccggaaca cggcgcgcca ctgcggctca ggattgcaaa
     6121 cgcgccaaca cctgggccat cggatagtac cgctgccccg attgcaaccg ccatggcagc
     6181 cactcaaccc gcgttcggcg aatcctcgcc ggcgactgaa cgcgccgccc aagttgctgt
     6241 tcctcaatac ggtgaacatt cgcaagtgaa agcggcgctt cgtcacggcg ggctcatcag
     6301 ggtctcctgc gcgaacacgc cggtgagcct gaacgacgca ccgcaagaac ctcaggagcc
     6361 gcacccgggc aacggtgacg ccgccacgtc ggccctgctc gcgctaagcg aggcgtttct
     6421 cgacggcgcg ggcattcacc ccgacaggca cgtcgaagcg ggcctcacgc ccgaattcat
     6481 gcgcgccctc ggcgcgatcg cgcgcgcgtt gagggcgcgg tagcgacacc gctgccgcac
     6541 ctcaggccgc ccgggcaggc ggctcgccca ccccgctctg ctatattctc tccatcccgt
     6601 ccctatcctt cactacgcgt ggaacaactt cccttatggg cgcagatctg cgccgtcttt
     6661 ctgctgctta tctgctccag cttcttttcg atttccgaaa cggcgatgat ggcgatcaat
     6721 cgccatcgcc tgaaacacct tgccaaccag aatgcgctcg gcgcgaagac cactcagggt
     6781 cttctggcgc acaccgacca gctgttgagc gtggtcctga tcggcaacaa tctcttcaac
     6841 acgatcattc cggtgttgac cacctcgatc gcactccata cgtttggccg caataacgtc
     6901 gtgctgtcca tcgcgaccgg catcgtcgcg tttctgatta ttgtgttcgc ggaaatcacg
     6961 ccgaagatcg tcggcgccac gttcccggag aagatcgcgc tgccggccag cctgctgatc
     7021 gcaccgatga tgcgtgtcgg caagccgctc gtctggttcg tcaatctgtt cgcgaacacg
     7081 atcttgcgcg tgctgcatat caataccaaa ggcgcgcacg atcagcggct ttccaccgaa
     7141 gagctgcgca ccatcgtgct cgaatcaggc agcttcatgc cgaccaagca ccgcagcatt
     7201 ctgttgaacc tgttcgacct cgagaacatc tccgtcgacg acgtgatgat cccgcgacgc
     7261 cgcatcgaag cgctcgattt cgacgcgccg ttcgaacaga tcctgcatca gctggaaacc
     7321 tgctatcaca acaagctgat cgtctatcaa ggcgacatcg accgggtgct cggcgtgctg
     7381 cacgtgcgca agactctcgc ggccctgcac aaccaggaac tggagcgcga gacgctgcgc
     7441 gagttgctgg ccgaaccgta tttcgtgccg accggcaccc ctgttttcca gcagttgcag
     7501 tatttccagg agagccgtca ccgcacggca ctgatcgtca acgaatacgg cgagttgcaa
     7561 ggactcgtca cgccggaaga catcatcgag gaactgatcg gcgaattcac cacgacgatt
     7621 ccgcgcagcg cgaattctcg cggcggctgg aacgagaagg gcgaatgcat cgtcgcgggc
     7681 agcatgccgc tgcgcgaact gaaccgctgg ctgcttctca caatgccgac ggacgggccg
     7741 aaaacgctca acggcttgat tctcgaaata ctcgaagaca ttcccgacgg cgacgtgtgc
     7801 gtccagatcg gcgagctgaa gctggaagtg atgcgcagcg acgaccaggc gattcgcact
     7861 gtcaagctgt tcagaccgcc ggtgcgagcg aaggcaagca aggcgctgcg cagataagtg
     7921 gtcgcagcgg gcgaggcggc cccaacctgt ccaggcgatc gaagctcgcg aagcctctca
     7981 g
//
LOCUS       DATASH010000098         4029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6936886,
            whole genome shotgun sequence.
ACCESSION   DATASH010000098 DATASH010000000
VERSION     DATASH010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6936886"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VHV99_02965"
     CDS             <1..271
                     /locus_tag="VHV99_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HEX3634444.1"
                     /translation="LPMLTALYWVLFVPRFHLRLRHCLLWLVYPIAYLVITFWRGSSS
                     DFYPYPFINVDVLGVRHVVVNSALLFGGFLMLMAVFVGINFRRRS"
     gene            481..756
                     /locus_tag="VHV99_02970"
     CDS             481..756
                     /locus_tag="VHV99_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEX3634445.1"
                     /translation="MDTNSAEGAVKEVAGKVQGAVGDVLGDTGTQLAGKAKELSGKAQ
                     QLCASTTSLVRDTTAESPFTTIAVVALAGFVAGALWSHGASGRDYRR"
     gene            complement(906..1718)
                     /locus_tag="VHV99_02975"
     CDS             complement(906..1718)
                     /locus_tag="VHV99_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013341737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX3634446.1"
                     /translation="MAKLRIATFNINGIRSRLPALLQWLEREAPDIVCLQELKAVDSA
                     FPVEQLREAGYASLWQGQSAWNGVAILSKGEAPIESRRGLRGFEEDTHSRYLEAAVGG
                     VLVACLYLPNGNPQPGPKFDYKLAWFDHLITHAAKLFKSGHPVVLAGDFNVVPTDQDI
                     YNPRSWLKDALLQPESRDRYQRLLAQGWTDSLRTHFGDERVYTFWDYFRRHWDTNSGL
                     RIDHILLSADLAPRLRDAGVDRWVRGEPHASDHAPTWVELDLGAGRRKKTSG"
     gene            complement(1756..1974)
                     /locus_tag="VHV99_02980"
     CDS             complement(1756..1974)
                     /locus_tag="VHV99_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634447.1"
                     /translation="MQFDYKHFHIDCRARHAEDGAYHARARISRVPQKHEHVQSHDSG
                     DIDAFSNEADAISCAKSWAMEWCDAATQ"
     gene            complement(2126..3607)
                     /locus_tag="VHV99_02985"
     CDS             complement(2126..3607)
                     /locus_tag="VHV99_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016348023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEX3634448.1"
                     /translation="MSDLNPVNPVAMTVFIAFFVLVTVIGFFAARWKRGDLTQLHEWG
                     LGGRQFGTVISWFLVGGDFYTAYTVIAVPALVYSVGAYGFFALPYTIIVYPFVFAVMP
                     KLWKIANAKNHITAADYVQGEYGGKWFPAAIAITGIVATMPYIALQLVGMQVVIKGLG
                     VTGEMPLIVAFVILALYTYASGLRAPAMIAFVKDIMIYIVVIAAVWLIPAKLGGYAHV
                     FDAADTYFKAKGGATGIILKPTQFTAYASLALGSALAAFMYPHTMTAVLSSSSANTVR
                     KNAIFLPAYTLLLGLIALLGYMAIAAGVHVKSASDMVPALFNTLFPSWFVGFAAAAIA
                     ISALVPAAIMSIGAANLFTRNLWRPLVSPNITPEAEATTAKMASLVVKFGALLFIVFL
                     PTQYAIDLQLLGGVWILQIFPAVVFSLYTRRLNTPGLFLGWLAGVGIGTGLAISQGLK
                     PVFALHIGDATWPLYIGLIALAANIVVSFVVSVLSPRRSVVAV"
     gene            complement(3604..3801)
                     /locus_tag="VHV99_02990"
     CDS             complement(3604..3801)
                     /locus_tag="VHV99_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3311 domain-containing protein"
                     /protein_id="HEX3634449.1"
                     /translation="METSQSAGRSWLWLILLIPYIALLWLPFYNDTRPSFAGFPFFYW
                     YQFLWVPLTSLLIYVVYRGVK"
BASE COUNT          884 a         1232 c         1166 g          747 t
ORIGIN      
        1 gctgcctatg ctgacagcac tttactgggt gctattcgtg ccgcgctttc atttgcggtt
       61 gcggcactgc ctgttatggc tggtctatcc cattgcctat ctcgtcatca ccttctggcg
      121 cgggagttcg tcggattttt atccgtatcc cttcatcaac gtcgatgtac ttggggtcag
      181 gcatgttgtc gtcaattccg ccctgttgtt tggcgggttt ttgatgttga tggcggtgtt
      241 cgtggggatt aattttcggc gtcgaagctg attgtttgtg tgctccggct ttcctgtgtg
      301 ttcgcgcttt acatgattgc acacggcgtc ggccgtgagt tgattccgtt cctcgggcgc
      361 gcttcttgcg ccccgtctga tggacgtaag ccggcaagca ggagcggcct gcgtgaacct
      421 cggtgtggcg acgttgcgcg gcaatggccc acacgtaaat ctgacaatgg gagcaaacgt
      481 atggacacca actcagcaga aggcgcagtc aaggaagtcg caggaaaggt gcaaggcgcc
      541 gtcggcgacg tgctaggtga tacaggcact caactggccg ggaaggccaa ggaactcagc
      601 ggcaaagccc aacaactttg cgcgagtacc accagccttg ttcgggatac gactgcggaa
      661 agccctttca ccaccattgc cgtcgtcgcg ctcgccggtt ttgtcgccgg tgcgctgtgg
      721 tcgcacgggg cgtcgggtcg cgattatcgc cggtaaattt cgacgcagct gatcagatgg
      781 tcgggcgatt gccagtaggc gatcgcccgg ctctgcttgt gcctcgctct ttggacgtcg
      841 gcgtgtggaa gttctcctgc cggcacccca gcaacacttg tacttcaaca cgatcgacga
      901 tgcgcctagc cgctcgtctt tttgcggcgt cccgcaccca ggtccagctc cacccacgtc
      961 ggcgcatggt cgctcgcgtg cggttcgccg cgtacccaac gatcgacgcc cgcgtcgcgt
     1021 aagcgcggcg cgagatccgc gctgagcagt atgtggtcga tgcgcaaacc cgagttcgtg
     1081 tcccaatggc gacgaaaata gtcccagaag gtgtacacgc gctcgtcgcc gaagtgggtc
     1141 cgcagcgaat cggtccaccc ttgcgcgagc agccgctgat agcggtcgcg gctttcgggt
     1201 tgcaacagcg cgtccttcaa ccatgaacgc gggttgtaga tgtcttggtc ggtcggaaca
     1261 acgttgaagt cgcccgccag caccaccgga tggccgctct tgaatagctt cgcggcgtgc
     1321 gtgatcagat ggtcgaacca cgcgagcttg tagtcgaact tggggcccgg ctgcggattg
     1381 ccgttcggca gatagagaca ggccaccagc acaccgccga cggccgcttc cagataacgg
     1441 ctatgcgtgt cctcttcgaa accacgcagg ccgcgccggc tttctatggg cgcctcccct
     1501 ttcgaaagaa tcgccacgcc gttccacgcg ctctggcctt gccagaggga agcgtagccc
     1561 gcctcgcgta gttgctcgac aggaaatgcg ctgtccacgg ccttcaattc ctgcaggcac
     1621 acaatgtccg gcgcttcacg ctcgagccat tgcagcagcg cgggcaagcg cgaacggata
     1681 ccgttgatgt tgaaggttgc gatgcgaagc ttggccacgg catggtcccc ctggaaggca
     1741 tcctgctgaa gcaccctact gggtcgccgc gtcgcaccac tccattgccc acgacttcgc
     1801 gcatgaaatg gcgtctgctt cgttcgaaaa ggcgtcgatg tcgcccgaat cgtgcgactg
     1861 gacatgctcg tgcttttgcg gcacgcggct gatcctggcc cgggcatggt aagcaccgtc
     1921 ttctgcgtgg cgagcccggc aatcgatgtg gaagtgcttg tagtcgaact gcatgacctc
     1981 tcctccctat aaacgcgcgg cacgtatcgc tcatttatag catcgcttat gcccgatcgg
     2041 atccctggtt ttccttctac acaaaaacaa aagccggcgc agcggagtaa gcaactccac
     2101 agcgccggcc agacagacag caaccttaaa ccgcgaccac actcctgcgc ggcgacaaga
     2161 ccgaaacaac gaaactaacc acgatgttag cggccaacgc gatcaagccg atataaagcg
     2221 gccaggtggc atcgccgata tgcagcgcaa ataccggctt gagcccttgc gaaatagcca
     2281 gtcccgtgcc gatcccgacc ccggcaagcc agccgaggaa caggcccggc gtattcagac
     2341 ggcgcgtgta caaggagaag acaacagccg ggaaaatctg caaaatccag accccgccca
     2401 gcagctgcaa atcaattgca tactgcgtcg gcaagaacac gataaacagc agcgcaccga
     2461 acttcaccac cagcgaagcc atcttcgcgg ttgttgcttc cgcctcaggc gtgatattcg
     2521 gcgagacgag aggacgccac aaattccgcg tgaacagatt ggccgcgcca atcgacatga
     2581 tcgctgcagg caccaacgcg ctgatcgcaa tcgccgcggc cgcaaagccg acgaaccacg
     2641 acgggaacag tgtgttgaag agtgccggca ccatgtccga ggcggacttc acatggacgc
     2701 cggccgcaat cgccatatag ccgagcaacg caatcagccc cagcaacagc gtgtacgccg
     2761 gcaggaaaat cgcattcttg cgcactgtat tcgccgacga ggacgacaac accgccgtca
     2821 tcgtgtgcgg atacatgaac gccgcgagtg ccgaacccaa agcaagcgag gcatacgcgg
     2881 tgaactgcgt cggtttcaga atgatgccgg tcgcgccgcc tttagccttg aaatacgtat
     2941 cggccgcatc gaacacatgc gcatagccgc cgagcttcgc gggaatcagc cagaccgccg
     3001 cgatcacgac gatatagatc atgatgtcct tgacgaacgc gatcatcgcc ggcgcccgca
     3061 agccgcttgc ataggtatac agcgcaagaa tcacgaaggc gacgatcagc ggcatttcgc
     3121 cggtcacgcc gagtcccttg atcaccacct gcatgcccac cagttgtagc gcgatatacg
     3181 gcatggtggc gacgataccg gtgatcgcga tcgcagcagg aaaccacttg ccgccatact
     3241 caccctgcac gtagtccgcc gccgtgatgt ggtttttcgc gttagcgatc ttccacagct
     3301 tcggcatcac cgcgaacacg aacggataga caatgatcgt gtacggcagc gcaaagaagc
     3361 catatgcgcc caccgaatac accagtgcgg gcaccgcgat cacggtgtag gcggtataga
     3421 agtcgccgcc cacgaggaac cacgaaatca ccgtgccgaa ttggcggcca cccaggcccc
     3481 attcgtgcaa ttgcgtcaga tcgccccgct tccagcgcgc ggcaaaaaag ccgatcacgg
     3541 tgacgagaac gaagaaagcg ataaagacgg tcatcgcgac cgggttcacg ggattcagat
     3601 cgctcatttg acacctcggt acacgacgta aatcagcagc gaggtcaacg gaacccacaa
     3661 gaactgatac cagtagaaga acggaaagcc ggcgaacgag gggcgcgtgt cgttatagaa
     3721 cggcagccat agcaaggcga tatacgggat cagcaagata agccagagcc atgaacggcc
     3781 ggcagattgg gaggtctcca caaacttctc ctaagtctat tattgaaact gcgcgccatg
     3841 acacgtggcc ggggcacgcg gaaaaccggt tagctgatat tagacggcag aaccagcgtt
     3901 cgctctttgc accgcgccag aacacggcag ctgaaaacgg ccgggcggat ggaacattca
     3961 tacgaatgag caccggccac accgcaagag gccggcgtcg cgtaaaaaaa cgccgcgcgc
     4021 gcgccggcg
//
LOCUS       DATASH010000099        11821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_804909,
            whole genome shotgun sequence.
ACCESSION   DATASH010000099 DATASH010000000
VERSION     DATASH010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 11821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11821
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804909"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="VHV99_02995"
     CDS             complement(<1..531)
                     /locus_tag="VHV99_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX3634450.1"
                     /translation="MTRRSNPLIGASALTLLISTAAWAAPSGTVAFLMPDQASTRYEQ
                     HDFPGFKAEMGKLCPDCKVLYQNANADVSTQQQQFNSVIAQGAKVIVLDPVDSTAAAS
                     LVHIAQNQGIKVIAYDRPVPSTPANYYVSFDNEGIGKAIAQSLVQHLKETNVPTSKGG
                     VLEVNGSPTDAAAGLIK"
     gene            complement(643..1650)
                     /locus_tag="VHV99_03000"
     CDS             complement(643..1650)
                     /locus_tag="VHV99_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016347159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX3634451.1"
                     /translation="MPTTLAATARIDARHANIAQPDLELVAVPRDESFKVWSHGYPYR
                     TVRWHFHPEYEIQLITSTTGKYFVGDYIGNFTPGNLVMVGSNLPHNWVSNVPHGEHVD
                     ERCLIVQFDAAFVKHAIKVFPEFKRVESLLEASRWGVLFAPETGVAAEPIMREMLGAK
                     GMRRITLFLALLDLLVQSVAPVKLASAAYCADPARYAETRINHVLSYIGKNLSQELRE
                     TELAELVGQSVSAFSRYFRRHTGVPFVQYVNRLRINLACQLLMSGELNITDICYRVGF
                     NNLSNFNRQFLSLKDMSPSRWRAYQQLNAASATEPPDAARASEKSGVAPGVMENPRTA
                     I"
     gene            1992..2384
                     /locus_tag="VHV99_03005"
     CDS             1992..2384
                     /locus_tag="VHV99_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014252839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEX3634452.1"
                     /translation="MFPVNGIVAVSTQRTVAQAAETLASLIEAKNLILFARIDFAADA
                     ARAGLVMNPSQLFIFGNPQAGTPLMQAAPTIALDLPLKILVWQEDGGQVWLSYNATSY
                     LQARHDAPAELMKAIDGIGALVQAAAAG"
     gene            complement(2391..2684)
                     /locus_tag="VHV99_03010"
     CDS             complement(2391..2684)
                     /locus_tag="VHV99_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEX3634453.1"
                     /translation="MSKGYWVSAYRATKDPSKLAAYAQLAPSAVAAAGGKFLVRGVAE
                     EVREQGLKERTVVIEFPTYEQAVAAYESDAYKKALEALGDGVERDLRIVRGTD"
     gene            complement(2892..3470)
                     /locus_tag="VHV99_03015"
     CDS             complement(2892..3470)
                     /locus_tag="VHV99_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEX3634454.1"
                     /translation="MSNRILLQLAAVFAPLSLVTIGGGQAIIADIQRQVVDVHGWMTH
                     AQFGVDFAISRMAPGPGSLLATLIGWQIAGFWGAVVATLALLAPTGILIYGVTHIWTR
                     YQGAAWQLALERGLRPVASGMILAAGWVLLKSLDGGFPAIAIALVSVAVLLSTRINPL
                     LLLLCGALLLFGMSELGASLPAMLQVHMASQT"
     gene            complement(3473..4039)
                     /locus_tag="VHV99_03020"
     CDS             complement(3473..4039)
                     /locus_tag="VHV99_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006406293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEX3634455.1"
                     /translation="MSQQIDNTSPPPTLSGLFFIFSRIGLTSFGGGLSGWLLREFVQD
                     RDWISEEDFLNGLAISQALPGINVKNMAIWIGHRLLGWRGALLAVAGIIIPPAIFIII
                     LGTVLSRLMQFPLAHILLAGAAAAATGLSLSMGVTTARRVPRRLLPLFFLAGTFVAVG
                     ILRLPLVWVVIGGGGLSVLFEYLKLRKT"
     gene            complement(4540..4938)
                     /locus_tag="VHV99_03025"
     CDS             complement(4540..4938)
                     /locus_tag="VHV99_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634456.1"
                     /translation="MAEYLNAATLLELAQHAEQRAAAPCMCTKTPLEGWQSQPLSLDE
                     TKFEEVGTLLPADDPEPTFSEYLQGKTSYWSADAPIAPRYFPYNRCAVWQCKLCNRLY
                     LRYTEGGGYFVDRRIRAVQSALIEDVPLAV"
     gene            complement(5040..5732)
                     /locus_tag="VHV99_03030"
     CDS             complement(5040..5732)
                     /locus_tag="VHV99_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEX3634457.1"
                     /translation="MNPASNPASNPIGWREYKSAPQVNPATLEVLRELAVSLLSDNMA
                     RASGMLGLRPYHQPKPMAGTAVTVHTRPGDNLAIHRAFDFCRPGDVLVIDGAGDLTQA
                     LMGDIMASYAESLGVQGLVIDGAIRDVGALSQRDFPVYARGVTHRGPYKNGPGEINVP
                     VTVGGMVVHPGDIIVGDEDGLLAISPADVEAVIEGARRQHAKETAALKSIAEGCFDRS
                     WVVPQRDRMMNN"
     gene            complement(5729..6769)
                     /locus_tag="VHV99_03035"
     CDS             complement(5729..6769)
                     /locus_tag="VHV99_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3634458.1"
                     /translation="MIIDCHGHYTTSPPQHEAWRAQQIAALKDGGSAPPRPVITDGQI
                     RESIETGQIRIQRERGTDLTIFSPRAAGMGHHLATAEANAVWSSESNNLIHRVVSLFP
                     RNFVGVCQLPQAPGVAPANCIAELRRCVEELGFIGCNLNPDPSGGHWSGLPLTDRSWY
                     PLYEAMVELDVPAMIHVSSSCNPNFHATGAHYINGDTSAFMQLLQGDLFADFPTLRFI
                     IPHGGGAVPYHWGRYRGLAQDMKKPLLSEYLMKNVFFDTCVYHQPGAELLAKVIPVEN
                     ILFASETIGAVQGIDPETGHYYDDTRRYIDAIEWLSAADRQRIYEDNARAVYPRLTHQ
                     LEQQFATQGATL"
     gene            complement(6894..8216)
                     /locus_tag="VHV99_03040"
     CDS             complement(6894..8216)
                     /locus_tag="VHV99_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3634459.1"
                     /translation="MTSFDETATMRKVYGRLMPLLFAMMFFNYLDRINIGFAALDMNK
                     QLGFSPAVFGFAGSIFFFGYMLLEVPSNLLLHRVGARRWIARILLTWGAVAAATAFVF
                     NDSSFYVLRFLLGVMEAGFLPGVAVYLTKWFPVRYRARAVGGYIIAGSFSAVLGGPIS
                     TTLMTYANGVLGLQGWQWMFILEGLPAMALGLLTLRIMTERPADANWLSDDEKQWLES
                     TLSAEREAVGGSTHFPLLRVASDIRVWSLACLFGCALVGIYGLFLWLPQIVKSFGHLS
                     NIEVGFLSAAPPLLGVLGTFLISRSSDRTGDRKKHLAFVYGMSALAIAGSAYAPNPVI
                     AYALLCLTGLFIYAGNPLFWSLASSFRTGAAGAATIALINTIAQFGGLVGPWSIGLVR
                     NATGSFKLALLTIAAFLVIATIIALVMRVAPSEDEVASLAPGDAAPRA"
     gene            complement(8479..9378)
                     /locus_tag="VHV99_03045"
     CDS             complement(8479..9378)
                     /locus_tag="VHV99_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3634460.1"
                     /translation="MNLRALQCFVILAEELNFSRAAERLHIAQPALSQQIRSLEERLG
                     TQLVDRTRRPLSLTEAGHYLCTEARQILGSLEQATLAAQEIGVGRRGWLSIGFTRSAM
                     YSVLPPALKAFHNAFPQVELKLFEMLTEEQTDGLRDMRIHIGIGRQPVAIDGCTSYTL
                     LRERVMVALEPDHPLAAHKTVRIAELADTPLILYPKHQNAQFKRSVQTLYRDAGVTPL
                     VAHQAYEIQTAIALVAAGLGVTFIGESVARHGRTDVVFRHLAGPGSSYRSTLAATFRT
                     DDASPHLRAFLACLPAPLADAAL"
     gene            complement(9375..10316)
                     /locus_tag="VHV99_03050"
     CDS             complement(9375..10316)
                     /locus_tag="VHV99_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3634461.1"
                     /translation="MTKSHPGITDLNLLRVFLAISDLRSLTAAAERLGLTQPAVSHAL
                     RRLRALFDDPLFVRTPTGMLPTDAALRLHAPLTQAFGIINMAVQQLAKFDPATAQRVF
                     RVSMSDMSEFYFLPPLLAMLDQNARGIHIEIANFPVDSVSTAMRSGKIDLALGYVPGL
                     DPGCVSKTLFVDEHVCVVRAGHPLRKQKPTKEDLAGLRYVYASTNATGHRMVEQWLEE
                     LNLSRDIVLRLPHFVVAPEIVQHTDLAVIFPRSIAQRFNRNKAFRILPLPFSLPPIEI
                     QVHSHSQFSADPGIAWLLDAIYTMFHQPAARTETRRT"
     gene            complement(10346..11062)
                     /locus_tag="VHV99_03055"
     CDS             complement(10346..11062)
                     /locus_tag="VHV99_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HEX3634462.1"
                     /translation="MEVCFFLLAHCDDTRQARSPIDPSLFEAASFEIPGLTRFVIHLP
                     AQLPESDPLSKTLASSPCCVLQWYFDDLGLLETALQTDGATHSIVARITKAAAVNLTF
                     AQQAMAVRPFAIPNPREPDPRAERCTYLVSYEGEAEDFNAWLTHYITHHPPLMAQLPG
                     IRELEIYTRLDVRSGLNIPRAAAMQRNKVVFDTPAALAAALASPIRAHMKQDFNAFPP
                     YSGPTPHFPMRSIYGNLNAT"
     gene            complement(11065..11619)
                     /gene="hpaC"
                     /locus_tag="VHV99_03060"
     CDS             complement(11065..11619)
                     /gene="hpaC"
                     /locus_tag="VHV99_03060"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HEX3634463.1"
                     /translation="MQQEKNVTIEDTRKRFRDAMACLPAAVNIITTDGPGGRCGITAS
                     AVCSVTDAPPTMLICINQASYVHDVLHRNRNVCINVLAAGCQDLARDFAGMTAGSMDE
                     RFGRHAWASGQASVPVLSDAIASLEGEIVDIKTVGSHSVMFAQIQHIALRSEGDGLIY
                     FGRQFHCLTRPAAAMPSVNAHTAR"
     gene            complement(11643..>11821)
                     /locus_tag="VHV99_03065"
     CDS             complement(11643..>11821)
                     /locus_tag="VHV99_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006397866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flavin-dependent monooxygenase"
                     /protein_id="HEX3634464.1"
                     /translation="VVMQAYQMAGIAAIYRENRLQRLVRDSIVVTQHAFLGEGTYDAS
                     GALFAGVPPVTPFP"
BASE COUNT         2337 a         3407 c         3800 g         2277 t
ORIGIN      
        1 cttgatcaat cccgctgcgg cgtcggtagg cgaaccattc acttcgagca cgccgccctt
       61 gctggtcggc acattggttt ctttcaaatg ctgaaccagc gattgcgcaa ttgccttgcc
      121 gatgccttcg ttgtcgaacg acacgtagta gttggccggc gtggagggca cgggccggtc
      181 atacgcgatc accttgatac cctggttctg cgcgatgtgg accagcgagg cggcggccgt
      241 cgaatcgacc ggatcgagga cgatcacctt ggcgccttgc gcgatcaccg agttgaactg
      301 ttgctgctgc gtcgagacgt cggcattggc gttctgatag agcaccttgc agtcgggaca
      361 gagcttgccc atttcggctt tgaagccggg gaagtcgtgc tgttcgtagc gggtcgacgc
      421 ctgatcaggc atcagaaagg cgaccgtgcc gctgggcgcg gcccatgccg cggtgctgat
      481 caacagggtc agggcgctgg cgccgattag tgggttcgaa cgtctggtca tcgaatgtct
      541 ccatttttag aaaatttccg gcgcgggtgc tgccgtggtc gggccgctgg tgaggcctct
      601 tggtgaaatt cgttagtagt tcgttagtgt ggtttgcagt gttcagatcg cggttcgtgg
      661 gttttccatg acacctggcg ctacgccgga cttttccgaa gcacgcgcgg cgtcaggcgg
      721 ttcagtggca ctcgccgcat tgagttgttg ataggcgcgc catcgcgaag gcgacatgtc
      781 cttcaaggac aggaactggc gattgaaatt cgagaggtta ttaaagccga cccgatagca
      841 gatatcggtg atgttcaatt cacccgacat cagtaattga cacgcgagat tgatgcgcag
      901 ccggttcacg tactgcacga acggcacgcc cgtgtgccgg cggaagtatc gcgagaaggc
      961 actcacgctt tgtcccacga gttcggccaa ctccgtttcg cgcaactctt gcgacaggtt
     1021 cttgccaata taagaaagca cgtgattgat acgcgtctcg gcatagcgtg ccgggtccgc
     1081 gcaataagca gcgctcgcca gcttcaccgg cgcgacgctt tgcacgagca agtcgagcaa
     1141 ggcaagaaac agcgtgatgc gccgcatgcc ttttgcgccc agcatctcgc gcatgatcgg
     1201 ttcggcagcc acacccgttt cgggtgcgaa cagcacaccc caacgcgaag cttcgagtaa
     1261 cgactcgacg cgcttgaact ccgggaatac cttgatcgca tgcttaacga acgctgcatc
     1321 gaattgcacg atcagacagc gttcatccac atgctcgcca tgtggcacat tgctcaccca
     1381 gttgtgcggc agattcgagc ccaccatcac gagattgccg ggcgtgaaat tgccgatgta
     1441 atcgccgacg aagtatttgc ccgtcgtcga cgtaatcagc tgaatttcgt attcgggatg
     1501 gaagtgccag cgcaccgtgc ggtagggata cccatgcgac cagaccttga aggactcgtc
     1561 gcgcggcacg gcgaccagtt ccagatccgg ctgggcgatg ttggcgtggc gcgcgtcgat
     1621 acgcgccgtg gcagccagcg tcgtgggcat ctgtcttcct cctgagcctg ttcagctttt
     1681 tttggcggtt cgcagagtcc atatcgcgct tcgccatggg agaaagttag tcctgtcgcg
     1741 gataccccgc cactgaaaat aagaccgcga actaatactt tattgcattc gggtaagtcc
     1801 gtagggcagc aggagtcaaa ttttgtgcag tgcggaagct gggctgcgga ctcagaatgg
     1861 atggatgccg aaaagcgtgt cagtgcttct cgcattcacg tgatcgtcga ctcactatgg
     1921 cagcgacacg cgctagtatt cgcttcgtcc tgtttcgtcc cgccttatcc tgtcacttcc
     1981 tggagaaatc catgttccct gtcaacggca tcgtcgccgt gtccacgcag cgcacagtcg
     2041 cgcaagccgc cgagacactc gcgtcgctga ttgaagcgaa aaacctcatc ttgttcgccc
     2101 gcattgattt cgcagcggat gccgcgcgcg cggggcttgt gatgaatccg tcgcagttgt
     2161 ttatattcgg caatccgcag gcgggaacgc cgctgatgca agccgcgccg accatcgcac
     2221 tcgacctgcc tttaaagata ttggtgtggc aggaggacgg cggccaggtg tggctctcgt
     2281 ataacgcaac gagttatctg caggcacggc atgacgcgcc tgcagagttg atgaaagcga
     2341 ttgatggcat cggcgcgctc gtgcaggcag cggcggccgg ctgacctgcc tcagtcggtg
     2401 ccgcgtacga tccgcagatc gcgctcgacg ccgtcgccca gggcttcgag cgctttcttg
     2461 taggcgtcgc tttcgtatgc ggcaacggcc tgctcgtagg tcgggaattc gatcacgaca
     2521 gtgcgttcct tcaagccctg ctcgcgaact tcctcggcga ctccgcgcac gaggaatttg
     2581 ccgccggcgg ctgccacggc agacggtgcc agctgtgcgt atgcggcgag tttcgacggg
     2641 tctttcgttg cgcggtatgc ggatacccag tatcccttgc tcatgtgaat ctccttgttg
     2701 gtggcgtgaa cggagtggtt gcgttgcaag caacgacgaa gaattatgtc atttatcggc
     2761 ctttcgcatc gacggatgaa acggccatct tgtgcgcgac tggctggtga tgccgctagt
     2821 cagcgcgctg atcgttctgt cgatggacgg cggttatcgc ggcttggtcg aaaagcctgt
     2881 ttgagctggc gttaggtctg gcttgccata tgcacctgaa gcatggcggg gaggctggcg
     2941 ccgagctcgg acatgccaaa caacagcaat gcgccgcaaa gcaacagaag caacgggtta
     3001 attcgcgtac tgagcagcac tgccaccgaa acgagagcaa tcgctatggc cggaaacccg
     3061 ccatcgagcg acttcagcag gacccatccc gcggcgagaa tcatgccgga tgccacaggc
     3121 cgcaaaccgc gctccagcgc cagttgccac gccgcaccct gataacgtgt ccagatgtgc
     3181 gtcacaccgt agatcagaat gccggtgggc gcgagcaatg ccagggttgc cacgacagca
     3241 ccccagaaac ccgcgatctg ccagccgatc aaagtagcca gcagcgaacc ggggccgggc
     3301 gccatgcgcg aaatggcaaa gtcgacgccg aactgggcat gcgtcatcca cccatgcacg
     3361 tccacgacct gccgttgaat atcggcaatg atggcctggc caccgccgat ggtaacgaga
     3421 gagagaggcg cgaaaactgc cgcaagctgt agaagaatgc gattcgacat ggctaggttt
     3481 tgcgcagttt gaggtattcg aaaagcacgc tcaggcctcc tccgcctatc acgacccaca
     3541 ccagcggcag ccgcaatatg ccgaccgcga cgaaggtgcc cgcgagaaaa aacagtggca
     3601 ggagtctgcg cggcacgcgg cgcgcggtgg tcacgcccat tgacaacgac aggccggtag
     3661 ccgcggcggc agcccctgca agcagaatgt gagcaagcgg gaattgcatc aaacggctga
     3721 gaaccgtgcc gagaatgatg atgaaaatgg cgggcgggat gatgatgccg gcaaccgcca
     3781 gcaaagcacc gcgccagccg agcagccggt ggccgatcca gatcgccatg tttttgacgt
     3841 tgatgcccgg cagcgcctgc gaaatggcga ggccgttcaa aaagtcttct tcactgatcc
     3901 agtcgcgatc ctggacgaat tcccgcaata gccacccgct cagcccgcca ccgaagctgg
     3961 tgagcccgat acggctgaat ataaagaaca acccggagag ggtgggcggt ggactcgtgt
     4021 tatctatttg ttgggacatc gaatcttcta gttagattga cgtatgacag gcgaaatagt
     4081 tgcgccagac gagcgggcca gatcatgtgt ggctggaaaa ttataacaac cggaggtttg
     4141 cttccggacg ccatttgcgg ctactcgatt gtattcggcg aattttggac gtcacgggtc
     4201 acccggttaa gtacctcgct gtcatcagga agtatcgggc cgtattcgcg gcaggaatgg
     4261 gggtggcgtg gaacaaggtg caaacgtgca atgcccaagg gggcattgcg gtctgcggta
     4321 tggaggactg gatacgcgaa aacctgctga cgcaggcgaa tgtttcgctt cagtagatcc
     4381 gctatcggcg ccgcgctcgg ggcgtttcga cgttccagtt ggggagctgg tttgaagggc
     4441 gtcccgtgaa gcaatcggct tttcaagcgg gtctatggga tgacgccgca gacgtatgcg
     4501 gcgctgaagc cggaccatca gggagcccat tgccgggcgt tacacagcaa gaggcacgtc
     4561 ctcgatcagc gcagactgta cggcgcgaat cctgcgatcg acgaagtagc cgccgccttc
     4621 cgtatagcgc agatacagcc ggttgcacaa tttgcactgc cagacggcac agcggttgta
     4681 cgggaagtag cgtggcgcga tgggcgcgtc ggcagaccaa tagctcgttt tgccctgcag
     4741 atactcgctg aacgtgggtt ccggatcgtc agccggtaga agcgtgccga cttcttcgaa
     4801 cttcgtttcg tcgagggaaa gcggctgcga ttgccagcct tccagtggtg tttttgtaca
     4861 catgcacggc gcagcagcgc gctgctccgc atgctgcgcg agttcgagga gggttgctgc
     4921 gttcagatat tccgccatga ggtacacgat tcctgcaggg ttgagccgat gatgccgccg
     4981 cgactcatgg tacgcggcgc accaatcgca tcatgcgcgc tccggcgccg cgcgtgaagt
     5041 cagttgttca tcatccggtc gcgctggggc acgacccacg aacggtcgaa acatccctcg
     5101 gcgatcgact tcaacgccgc cgtttctttc gcatgctgcc gccgtgcgcc ttcgatcacc
     5161 gcttccacgt cggcgggcga gatcgcgagc aaaccgtctt catcgccgac gatgatatcg
     5221 ccgggatgaa ccaccatccc gccgaccgtg accggcacgt tgatctcgcc cggtccgttt
     5281 ttgtaaggcc cacggtgcgt cacgccacgc gcatacaccg gaaaatcgcg ctgactcagc
     5341 gcgccgacgt cgcggatcgc tccgtcgatc acaaggccct gcacgcccag gctttcggcg
     5401 tagctggcca tgatgtcgcc catcagtgct tgggtcagat ctccggcgcc gtcgatcacg
     5461 agcacgtcgc ccggccggca aaagtcgaac gcgcgatgaa tcgcgaggtt gtcgccggga
     5521 cgggtatgca ccgtgaccgc ggtgccggcc atcggcttgg gctgatggta cgggcgcaag
     5581 ccgagcatgc cgctcgcgcg cgccatgttg tcgctgagca gtgaaaccgc cagttcacgc
     5641 aagacctcga gcgttgccgg gtttacctga ggcgcggatt tgtattcgcg ccagccgatc
     5701 ggatttgaag cgggattcga agcgggattc atagcgttgc cccctgggtg gcgaattgct
     5761 gttcgagttg atgggtcaac cgcgggtaca cggcgcgggc gttgtcttcg taaatacgct
     5821 gacggtcggc ggcactgagc cattcgatcg cgtcgatata gcgccgcgtg tcgtcgtagt
     5881 agtggccggt ttccggatcg ataccctgga ccgcgccgat ggtttccgac gcgaacagaa
     5941 tgttctcgac gggaatgacc ttggcgagca gttcggcgcc gggctggtga tagacgcagg
     6001 tatcgaagaa cacgttcttc atcaggtact cgctgaggag cggctttttc atgtcctgcg
     6061 cgagcccgcg gtagcgtccc cagtgataag gcacggcgcc gccgccgtgc gggatgatga
     6121 agcgcagggt cgggaagtcg gcgaacagat cgccctgcag caattgcatg aacgcggagg
     6181 tgtcgccgtt gatgtaatgc gcgcccgtcg catggaaatt cggattgcac gacgacgaca
     6241 cgtgaatcat cgctggcacg tcgagttcca ccatcgcttc atagagcgga taccacgagc
     6301 ggtccgtgag cggcaaccct gaccagtggc cgccggaagg atccgggttc agattgcagc
     6361 cgatgaagcc cagttcctcg acgcagcgcc gcaattcggc gatgcaattg gccggcgcca
     6421 cgccgggcgc ctgcggcaac tggcatacac caacgaagtt gcgcggaaac agcgaaacca
     6481 cgcggtggat aaggttgttg gattcgctcg accagaccgc attggcttcg gccgtggcga
     6541 ggtgatgccc catgccggcg gcgcgcggcg agaagatcgt gagatcggta ccgcgttcgc
     6601 gctggatgcg aatctggccg gtttcgatac tctcgcgaat ctggccgtcg gtaatgacgg
     6661 ggcgcggcgg cgctgacccg ccgtccttca gcgcggcgat ctgctgcgcg cgccaggcct
     6721 cgtgctgcgg cggcgaagtg gtgtagtgac cgtggcaatc gatgatcatg gtgtgaaaac
     6781 ccgttgggtg tccttatggg tgcttacctg tgctctacgt gcttgatgtg ctttatctgc
     6841 ttaagcgctg acatggcatg cgcggcacgt tcgctattcg tcggtcgggg tgctcaggca
     6901 cgtggggcgg catcgccggg tgcgagcgag gcgacttcgt cctcgctcgg cgcaacgcgc
     6961 atcacgagtg cgatgatcgt cgcgattacc agaaacgccg cgatcgtcag caaggccagc
     7021 ttgaagctgc cggtcgcatt gcgcaccagc ccgatgctcc aggggccgac cagcccgccg
     7081 aactgcgcga tggtgttgat cagcgcgatg gtcgcggcgc ccgcggcgcc ggttctgaac
     7141 gacgacgcga ggctccagaa cagcggattg cctgcataga tgaacaaccc cgtcagacac
     7201 agcaacgcgt aagcgatcac ggggttcggt gcgtacgcgc tgccggcaat cgccagcgcg
     7261 ctcatgccgt acacgaaggc gaggtgcttc ttgcggtcgc cggtgcggtc ggaactgcgg
     7321 ctgatcagga aggtgcccag cacgcccagc agcggcggcg cggcggacag aaagccgacc
     7381 tcgatattgc tgagatggcc gaaactcttg acgatttgcg gcagccacag gaacaggcca
     7441 taaataccca ccagcgcgca gccgaacaga caggcgaggc tccatacgcg gatgtcactc
     7501 gccacgcgca gcagcgggaa atgggtgctc ccgccgaccg cctcgcgttc ggcggagagc
     7561 gtcgattcga gccactgctt ttcgtcgtcg gaaagccaat tggcgtcggc cgggcgttcg
     7621 gtcatgatgc gcagcgtcag cagaccgagc gccatcgccg ggaggccttc gaggatgaac
     7681 atccattgcc atccctgcaa accgagcacg ccgttcgcgt aggtcatcaa ggtcgtcgag
     7741 atcgggccgc ccagcaccgc cgaaaacgat cccgcgatga tgtagccgcc caccgcgcga
     7801 gcccggtaac gcaccggaaa ccatttggtc aggtaaaccg ccacgcccgg caaaaagccc
     7861 gcttccatca cacccagcag aaagcgcaag acgtagaagc tcgaatcgtt gaagacaaaa
     7921 gcggtggccg ccgcgaccgc gccccacgtc agcagaatgc gtgcgatcca gcggcgtgcg
     7981 ccgacccgat gcagcaacag gttgctcggc acttccagca acatgtagcc gaaaaagaag
     8041 atgctgcccg cgaagccgaa cacggctgga ctgaaaccga gctgtttatt catgtccagc
     8101 gcggcgaagc cgatattgat ccggtccaga tagttgaaga acatcatcgc gaagagcagg
     8161 ggcattaagc gcccatacac tttgcgcatg gtggcggttt cgtcgaatga ggtcatgctg
     8221 tctccgtacc atgctgcgag ggctggcgcc gttgcagcgt gtttatagtc ggcctgccga
     8281 tcgaacgccg gcagtgtccc gttggccgcg cgttttggcg gccggacttc agatgtcagc
     8341 gcgcgtgctg gcgggcgctg attcgctcat gggctcatcg gcatgaaaca gagcgcgagg
     8401 ctgaagcatg aaaccaatga taagaacgct ctgacgaaga gtccaagacc gcttgcttat
     8461 gcttgtatag gtttttgctt atagtgcggc gtcagcaagc ggagccggga ggcaggcaag
     8521 gaaagcgcgc agatgcggag aagcgtcgtc ggtgcgaaag gtggccgcga gcgtggatcg
     8581 gtacgacgaa ccgggaccgg cgagatggcg gaacacgacg tcggtgcgcc cgtgccgtgc
     8641 caccgattcg ccgatgaacg tgacgccgag acccgcggcc accagcgcaa tcgcggtctg
     8701 aatttcatag gcctggtgag cgacgagcgg tgtcacgccc gcatcgcgat acagcgtttg
     8761 caccgagcgt ttgaactggg cgttctggtg cttcggataa agaatcaacg gggtatcggc
     8821 gagctcggca attcgcacgg tcttgtgcgc tgcgagcgga tggtccggtt caagcgcgac
     8881 catcacgcgc tcgcgcagca gcgtatagga cgtgcaccca tcgatcgcca ccggctgccg
     8941 cccgatgccg atgtgaatgc gcatgtcgcg caagccatcg gtttgttcct cggtgagcat
     9001 ctcgaagagt ttgagttcga cctgcgggaa cgcgttgtga aacgccttga gcgcgggcgg
     9061 caggacgctg tacatcgccg agcgcgtgaa gccgatgctg agccagccgc gccggccgac
     9121 gccgatttcc tgggcagcga gtgttgcctg ttccaacgaa cccaggatct gacgcgcctc
     9181 cgtgcagaga tagtggccgg cttcggtcag actcaacggg cgccgtgtcc ggtcgaccag
     9241 ttgcgtgccg agccgctctt cgagcgagcg gatctgctgg ctgagtgccg gctgggcgat
     9301 atgcaagcgc tcggcggcgc ggctgaaatt gagctcctca gcaagaatca cgaagcactg
     9361 taacgccctg aggttcatgt acgcctcgtc tccgtgcggg ccgccggctg atggaacatg
     9421 gtgtaaatcg catcgaggag ccatgcaatg cccgggtccg ccgaaaattg cgagtgcgaa
     9481 tggacctgaa tttcgatggg cggcagggag aacggtaacg gaaggatgcg aaaggccttg
     9541 ttgcggttaa agcgctgcgc gatgctcctg ggaaaaataa cggcgagatc ggtgtgttga
     9601 acgatctcgg gcgccaccac gaaatggggc agacgcaaga cgatatcccg gctgagattc
     9661 agttcttcca accattgctc gaccatcctg tgcccggtgg cattcgtgct tgcgtagacg
     9721 tagcgcaagc ccgcgaggtc ttccttggtc ggcttctgtt tgcgtagcgg gtggcctgcc
     9781 cgcaccacac agacatgttc gtcgacaaag agggtcttgc tcacgcaacc cggatcgagg
     9841 ccaggcacgt agccgagcgc cagatcgatc ttgccgctgc gcatggcggt gctcaccgag
     9901 tcgactggga agttcgcgat ctcgatgtgg atgccgcgtg cattctggtc gagcatcgcg
     9961 agaaggggcg gcagaaaata gaactccgac atgtccgaca tggaaacgcg gaatacccgt
    10021 tgcgcggtgg cggggtcgaa tttcgcgagt tgctggaccg ccatgttgat gatcccgaac
    10081 gcctgcgtga ggggcgcgtg caagcgcaag gcggcgtcgg tcgggagcat gccggtgggt
    10141 gttcgaacga agagcgggtc gtcgaataat gcacgcaacc ggcgaagggc gtggctgacc
    10201 gccggttgtg tgagtccgag gcgctcagcc gcggcggtca gactgcgcag gtcggagatg
    10261 gccagaaaca cgcgcagcag attcaaatcc gtaatgccgg ggtgcgactt tgtcatgacg
    10321 ttcggatcgc ggcaataagt ggcgtttatg tcgcattcag attaccgtaa atgctgcgca
    10381 tagggaaatg cggggtgggt ccgctgtacg gtggaaacgc gttgaaatcc tgtttcatgt
    10441 gggcacggat cggcgaggcc agcgccgcgg cgagtgccgc gggcgtgtcg aagacgacct
    10501 tgttacgctg catggcggcg gcgcgcggaa tgttcaggcc ggatcggaca tcgagccgcg
    10561 tgtagatttc cagctcgcga atcccgggca gttgcgccat tagcggcgga tgatgagtga
    10621 tgtagtgcgt gagccatgcg ttgaaatctt ccgcttcgcc ttcgtagctg acgaggtagg
    10681 tgcatcgttc agcgcgaggg tcgggctcgc gtggattcgg tatggcgaat ggcctgactg
    10741 ccatggcttg ctgtgcgaac gtgagattga ctgcggcagc tttcgtgatg cgagccacga
    10801 tgctgtgcgt cgcgccatcg gtttgcagtg ctgtttcgag caaaccgaga tcgtcgaaat
    10861 accattgcaa gacgcagcag gggctcgaag caagggtctt cgatagagga tcgctttccg
    10921 gcaactgcgc ggggagatgg atcacgaaac gcgtcaaccc tggtatttca aacgatgccg
    10981 cttcaaacaa cgacgggtcg atgggcgagc gcgcctggcg cgtgtcgtcg caatgggcga
    11041 gaagaaagaa gcagacttcc acgtttacct cgcggtatgc gcgttaacgg acggcattgc
    11101 cgctgccggc cgcgtcaggc aatggaactg gcgtccgaaa tagatgagcc cgtcaccttc
    11161 ggatcgcagc gcaatgtgct ggatctgcgc gaacatgacg gagtgcgagc cgactgtctt
    11221 gatgtcgacg atttcgcctt ccaggctcgc aatcgcatcc gacaggacgg gcaccgaggc
    11281 ttgtccgctc gcccatgcat gtcgtccgaa gcgctcgtcc atcgaaccgg ccgtcatgcc
    11341 ggcaaaatca cgcgcgaggt cctggcaccc ggcggcgagc acattgatgc acacgttgcg
    11401 gttgcgatgc agtacgtcgt ggacgtagct ggcctgattg atacagatga gcatggtcgg
    11461 cggcgcgtcc gttaccgagc acacggcgct cgcggtgatg ccgcagcggc cgccgggtcc
    11521 gtccgtggtg atgatgttga cggcggcggg caggcacgcc atcgcatcgc ggaaacgctt
    11581 tctggtgtct tcgatcgtca cgttcttttc ctgttgcatg gcttgaggca gggcgtgccg
    11641 agtcacggaa aaggcgtgac gggcggcacg ccggcgaaca atgcgccgga tgcgtcgtac
    11701 gtgccttcgc ccagaaacgc atgctgcgta accacgatcg aatcccgcac gagacgctgc
    11761 aaccggttct cgcgatagat cgcggcgatg ccggccatct gataagcctg catcaccact
    11821 t
//
LOCUS       DATASH010000100         7831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1793792,
            whole genome shotgun sequence.
ACCESSION   DATASH010000100 DATASH010000000
VERSION     DATASH010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7831
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1793792"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /locus_tag="VHV99_03070"
     CDS             complement(<1..826)
                     /locus_tag="VHV99_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HEX3634465.1"
                     /translation="MKLSFVQKLWLPLVLSLLCLAGISIYNAYQTREMRLDERKADLK
                     HASEIAQSVVKTFGDEAAAGAMPVAEAQKRALDSIRNIRYGAGGYFGILNSQLTIVMQ
                     PMQPSMNGKNVSDYKDPNGVYMFREMVAVIKRDGQGFTEYEFPKPGATEASPKISYNA
                     TYQPWDWILTTGLYIDDLDAEFRTTLYQSLGILVILAGALSAVVVLLNRGILRSLGGE
                     PSYAAGIAIQIANNDLTAEVKTAPHDRSSLLFSMKRMQEQLTHTIGTIKVSADSIAT"
     gene            1171..1965
                     /locus_tag="VHV99_03075"
     CDS             1171..1965
                     /locus_tag="VHV99_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HEX3634466.1"
                     /translation="MTTSIRNGQKDGQPAMHTPSVVSAEAWEAAREQMLVKEKAHTRA
                     RDALAAERRRMPWIAVDKAYAFEGPAGKASLFDLFEGRRQLIVYRAFFEPGVFGWPEH
                     ACRGCSMVADQVAHLAHLNARDTTLVFVSRAPQADIARLKARMGWEIPWFTVTDSFDT
                     DFGVAEWHGTNVFYRDGDRVFRTYFINNRGDEQMGGTWNYLDMTPLGRQEVWEDSPAG
                     YPQTPTYKWWNWHDSYVPDAEPDKKWVEVSDAGEAAFRDQEGNAKP"
     gene            complement(2113..2805)
                     /locus_tag="VHV99_03080"
     CDS             complement(2113..2805)
                     /locus_tag="VHV99_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018434566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HEX3634467.1"
                     /translation="MGSTNAVVAAAVVFVGSHFVLSHPLRRPLVRALGEVGFLGAYSL
                     VAILTFGWLIWAYLKAPLTAPLWPVGDVLWALVTVVMLIASILLMGSLIRNPALPTGG
                     RPGSLPEAALGVFAVTRHPMMWSFALWGLCHAAVFPVARSIILAAAIVVLALVGAALQ
                     DRKKEQLQPDLWPAWESKTSYVPFAAIAAGRARLGGFGLHALLGGLVVWLVATWAHRP
                     LTGWAAGIWRWL"
     gene            2898..3317
                     /locus_tag="VHV99_03085"
     CDS             2898..3317
                     /locus_tag="VHV99_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634468.1"
                     /translation="MRFGIEGAFNWTFRDGNSRRYSGAGICHCSISSTEDYYFACSKT
                     RDGRTTQTHGNTKRVVTRPELLRIRNRTNAPGPSLRDGCIDSFVTECHLYRASFLPGV
                     KPEGKVQAKGGTGLSAVRLRAVPDTINTVTATDFPQR"
     gene            3314..4243
                     /locus_tag="VHV99_03090"
     CDS             3314..4243
                     /locus_tag="VHV99_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3634469.1"
                     /translation="MTRRAENLRFDLTTLRLFEATAELGAVTKAADRLALAPAAASRR
                     ISELESQFGVVLFERRPHGMTLTDAGRTLLAHARSMIHSAARMHDDASAFRQGDRGVV
                     KIAACTSAVLQFLAEDVQRCHATHPGIRIDLQELNSHGVMQALSRNVVDIGIYESSLG
                     HLEFPTRMYREDRLVVLTTADHPLAELKRVTLNDLFAYDVIGLTEGSAISIALGRLAN
                     QRARVLHMRIRVGSFDSMTAMVAAGVGIGVMPLEVARVLASGKRFRVLPIEGEWATRQ
                     FLLCHLPPESISSSALSVIELLVAQVSQNSKPA"
     gene            4344..5324
                     /locus_tag="VHV99_03095"
     CDS             4344..5324
                     /locus_tag="VHV99_03095"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEX3634470.1"
                     /translation="MSSQPVRIVLSIGDPAGIGAEIIVRLLADPSIRQKAHVTLIGNH
                     DAIARGASAAGVELPQAADWLDIPSWAGSDAVFAPGVISEASGRFMLDSLRLAVQYVG
                     DGRADAVCFGPLNKSALRAGGMMEEDEMRWFAKLLDHQGVCGELNVLEPLWTARVTSH
                     MPLREVSAHLSPRAVADAIRMLTEALRASGNAAPRIGVCGLNPHNGDHGNYGTEESDI
                     IEPGMALAESQGFVSTGPYPADTIFLRARAGELDGIVTMYHDQGQIATKLLGFDVGVT
                     VEGGLPIPLTTPAHGTAYDIVGQGKASGTAMARAFDVACRMVRSRQTAAV"
     gene            5407..6783
                     /locus_tag="VHV99_03100"
     CDS             5407..6783
                     /locus_tag="VHV99_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3634471.1"
                     /translation="MNPALTRSPRTHVQQGVLALLCAMSFILYLDRVNLAAAAGPIKA
                     ELGLSNTTLGVAFSAFGYTYAIFQIIGGWLADRMGARRTLLICGSIWVVATIATGMVG
                     GLASLCAARLLLGIGEGAALPAQARAIANWFHTKDRGFVQGVTHSSSRLGNAAAPTLV
                     VLLATLYSWRLSFILVGGFTAVWIVVWMVYYRDDPRAHGRVNEAELACLPVGMVHESQ
                     ELQGTPWRRILRRMSPTIFVYFCQVWSNTLFFSWVPLFFLHGYGLNLKESAIFSSGVF
                     LAGVVGDVLGGFLSDRVLKRTGNLKLARQGVIAFSLLGAFVFMIPVMISRDLTVVTLC
                     LSGAFFMLELTIGPIWAVPMDVAERHAGTASGMLNTGAALATIISPVVFGAVIDATGN
                     WSLPFVGSLLFLLLGAVMTLKIRPDLDIGEGLAPSGNADPIPAQRSQRMNQGGAEKSI
                     TVMDGDAS"
     gene            complement(6900..7820)
                     /locus_tag="VHV99_03105"
     CDS             complement(6900..7820)
                     /locus_tag="VHV99_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3634472.1"
                     /translation="MIRFDDLALFVRTAALGSFSKAAREAALLPGQVSAAIQRLEREL
                     DVRLFARSTRSLRLTAQGEEYLPYAKQVLATLQEGRECLQGAADALQGPLQIAAPSDL
                     GRNVLLPWITAFREAHPKLSVRLFLSDQLTDMFRDPVEIAIRYGRADNASYVALPLAS
                     DNRRVLVASPAYLQRHGRPRTLDELAGHHCLLYVLGGRVYDKWMFPVGGELRQVAVAG
                     SLFCDDADVARRWAIEGQGIAYKSWLDVCADVQTGRLEILMPEQPGEAAPLNLICTHR
                     KQFFPAIRQLHEVLKERVSALTALLPKYAP"
BASE COUNT         1477 a         2420 c         2443 g         1491 t
ORIGIN      
        1 cagtcgcaat ggagtcggcc gacaccttga tcgtgccgat cgtgtgcgtg agctgctcct
       61 gcatgcgctt catcgagaac agcaggctcg agcggtcatg gggtgccgtc ttcacctcgg
      121 cagtcagatc gttgttggca atctggatgg cgatcccggc ggcataggac ggctcgccgc
      181 ccagtgagcg gaggatgccg cggttgagca gcaccacgac agctgacaga gcaccggcca
      241 gtatcaccag aatgccgagg ctctgataca gcgtcgtgcg gaattccgca tcgaggtcgt
      301 cgatatagag ccccgtggtg agaatccagt cccacggctg gtaggtcgcg ttgtaggaaa
      361 ttttcgggga agcctcggtt gctcctggct tcggaaactc atactcagta aagccctggc
      421 catcacgctt gatgacagcg accatctccc tgaacatgta gacaccgttc ggatccttgt
      481 agtcgctgac gttcttgcca ttcatactcg gttgcatcgg ttgcatcacg atggtcaact
      541 gcgaattcaa gatcccaaaa tagccgcctg cgccatagcg gatgttccgg atgctgtcca
      601 atgcccgctt ttgggcttct gccacgggca tggcgccggc ggctgcctca tcgccaaagg
      661 tcttgaccac actctgggcg atctccgacg cgtgtttcag gtccgccttg cgctcgtcca
      721 gacgcatctc tcgagtctgg taagcgttat agatcgatat tccagcgaga cacagcagac
      781 tcaggaccag tggcagccag agtttttgaa caaaagacag tttcatagga tgacgaatct
      841 ttcagtttgg ctagtgcaag tgactacgcg aatattgaaa attgactacg agaaaactgt
      901 ataacggcac ttaaccggaa aacttcagat aaataaaatg cgtctgtgcg aactgttctt
      961 caatcgcagc gattaccggc gacgcaccgg gcacatgcat tgcagcgatg acctgaaatc
     1021 acatcggaat gccgtcacac ttgttactcc cagtgttcga tctccggtgc aaaactgtct
     1081 cacggccaac gacgagcgtt gagcatcgaa tggggccgcg cttcagccat gaggggcggc
     1141 aattcaccca aaccggagat agcaaaaatc atgacgacct caatcaggaa cggacagaaa
     1201 gacggacagc ccgccatgca tacaccatcg gtcgtgtcgg ccgaggcgtg ggaggcggcc
     1261 cgcgagcaga tgctagtgaa agagaaagcc catacccgtg cccgtgacgc gctggccgcc
     1321 gagcgccggc gcatgccgtg gatcgccgtg gacaaggcgt atgcattcga ggggccggcg
     1381 ggcaaggcca gcctgttcga cttgttcgaa ggtcggcgtc agttgatcgt ctaccgcgcc
     1441 ttcttcgagc cgggcgtgtt cggctggccc gagcatgcgt gccggggttg ctccatggtg
     1501 gccgatcagg tagcgcacct cgctcacctg aacgcccgtg acaccacgct cgtgttcgtc
     1561 tcgcgtgcgc ctcaggcgga catcgcacgg ctgaaggcgc ggatgggctg ggagataccg
     1621 tggttcaccg tcacggatag cttcgacacc gacttcggcg tggcggagtg gcatggcacg
     1681 aacgtgttct accgcgatgg cgaccgcgtg ttccgcacgt acttcatcaa caaccgcggc
     1741 gacgagcaga tgggcggtac ctggaactac ctcgacatga cgccgctggg gcggcaggag
     1801 gtttgggagg actcgcccgc gggctatcct cagacgccga cctacaagtg gtggaactgg
     1861 cacgacagct acgtgccgga cgccgaaccc gacaagaagt gggttgaggt gtcggacgcc
     1921 ggcgaggcgg cgttccggga ccaggaaggg aacgcgaaac cgtgagccaa gccggttcca
     1981 acggaaccag cggatggcga cggcgggtct gagggggcca cgatcggcaa cggcgcgatg
     2041 ggatatgacc ggccatgacg cgaaggtccg cgatgaagag cggtcgcgca ggcgtgaccg
     2101 cctggaacgg ggttacagcc agcgccagat gcccgcggcc cagccggtga gcggcctatg
     2161 agcccaagtc gcgacgagcc agacgacgag gccgcccaac agcgcgtgca agccgaaccc
     2221 gcccagccgc gcacggccag cggcgatcgc cgcgaacggc acgtagctcg tcttcgattc
     2281 ccacgcgggc cagagatcgg gctggagctg ctccttcttc cggtcctgca gtgcagcgcc
     2341 gacgagtgcg aggacgacga tagccgctgc aaggatgatg ctcctggcca ccgggaacac
     2401 cgcggcgtga catagtcccc aaagcgcgaa cgaccacatc attgggtgac gcgtcacggc
     2461 gaacacgccg agcgccgctt ccggcaacga gccaggccgc ccgccggtgg gaagcgccgg
     2521 attgcggatc agagagccca tcaggaggat cgacgcgatc agcatgacga cggtcacaag
     2581 cgcccagagc acgtctccta cgggccatag cggtgccgtg aggggcgcct tcaggtaggc
     2641 ccagatcagc cagccgaacg tcaggatcgc gaccagcgaa taggcgccca ggaaccccac
     2701 ttcgccgagt gcgcgtacca aaggcctgcg cagcggatgg gacaagacaa agtgactgcc
     2761 cacgaagacg actgccgccg ccacaaccgc gttggtgctt cccataggtc gggctcctca
     2821 agttatattg tcggctgacc gggttgccct taataccaag cttcgtggca ggcccggctc
     2881 gatgcagccg gcatggcgtg cgcttcggta ttgaaggggc attcaattgg acatttcgtg
     2941 atggcaactc acgccgctat agcggggcgg gaatctgcca ttgttcgata tccagtactg
     3001 aagattacta ctttgcgtgc agcaaaacgc gcgatggacg aacgacacaa acacacggca
     3061 atacgaagcg cgtcgtcaca agaccagagt tgctacgtat tcgtaatcga acaaacgcgc
     3121 cggggccgag ccttcgggat ggttgcattg attcattcgt tacggaatgc catctctatc
     3181 gcgcttcttt tttacctggc gtgaagccgg aggggaaggt gcaggcgaaa ggcggcacag
     3241 gactgagcgc ggttcggctg cgggccgtgc ccgatacaat caacaccgtt accgccaccg
     3301 atttcccaca aagatgactc gccgcgctga aaacctccgt tttgatctta cgacgctccg
     3361 tctattcgaa gcgacagccg aactaggcgc cgtaaccaag gcggcggacc gtcttgcgct
     3421 tgcgccggcg gcggcttcca ggcgcatcag cgaactggaa tcgcagtttg gtgtagtact
     3481 gttcgagcgc cgccctcacg gcatgacgct caccgacgca ggtcgcacct tgcttgccca
     3541 cgcacgcagc atgattcatt cggccgcgcg catgcacgac gacgcatccg ccttccgcca
     3601 gggcgatcgt ggcgtggtga aaattgcggc ttgtacgtcc gctgttcttc agttcctcgc
     3661 ggaagatgtg cagcgatgcc atgcaacgca tcccggcatc cggatcgatc tgcaagaatt
     3721 gaacagtcac ggcgtcatgc aggcgctttc acgcaatgtc gtcgatatcg gcatctacga
     3781 gtcctcgctg ggtcacctcg aatttccgac ccggatgtat cgcgaggacc ggctcgtcgt
     3841 gctgacgacc gccgatcatc cgctcgccga actcaagcgg gtcacgctca atgacctgtt
     3901 cgcctacgac gtgattggcc tgaccgaggg ttcagcgata tcaatcgcgc tcggccgtct
     3961 cgcgaaccaa cgcgctcgcg tcctgcatat gcgcatccgt gtgggcagct tcgacagcat
     4021 gaccgccatg gtggccgcgg gcgtcgggat cggcgtcatg ccgctcgaag tcgcgcgggt
     4081 gctggcgagc ggcaagcgct tccgcgtgct gccaatcgaa ggcgagtggg ccacgcggca
     4141 attcctgttg tgccacttgc cgcctgaatc catctcatcc tccgcgctgt ccgtgatcga
     4201 gctcctcgtc gcgcaggttt cgcagaattc gaaacctgcg tagcctaatg agcgatggcg
     4261 ctggccgccg atccttctta tctttaggcg tcgtcgctac ctggaaagcg tagcgctccc
     4321 atcgttctcg ctgctggagt tccatgtctt cgcaacccgt tcgaattgtc cttagcatcg
     4381 gcgatcccgc tggcatcggc gccgagatca tcgtgcgcct gcttgcggat ccttccattc
     4441 gccaaaaggc gcatgtcaca ctgattggca atcacgacgc catcgcgcgc ggcgcgtcgg
     4501 ctgccggcgt cgagttgccg caagctgccg actggctcga tattccatcc tgggctggca
     4561 gcgacgcggt gttcgccccg ggcgtcatca gcgaggccag tgggcgcttc atgctggatt
     4621 cgctgcggct cgccgtgcaa tacgttggcg atgggcgcgc cgacgccgtg tgtttcgggc
     4681 cgctcaacaa gagcgcgctg cgcgcgggcg gcatgatgga agaagacgaa atgcgctggt
     4741 tcgcgaagct gctggatcac cagggcgtat gcggcgaact gaacgtgctc gaaccgctat
     4801 ggacggcgcg cgtcacctcg cacatgccgc tcagggaagt ctccgcgcat ctgtcgcccc
     4861 gagccgtggc cgatgcgatc cgtatgctga ctgaagcgct tcgggcgtcc ggaaacgctg
     4921 cgcctcgcat cggcgtgtgc gggctcaatc cgcataacgg cgaccacggc aactatggca
     4981 ccgaagagag cgacattatc gaaccgggta tggcgctcgc ggagtcgcag gggtttgtat
     5041 cgacgggtcc gtatcccgcg gacacgatct ttctgcgtgc gcgagccggc gaactcgacg
     5101 gcattgtcac gatgtatcac gaccaggggc agatcgcgac caagctgctc ggcttcgacg
     5161 taggcgtcac cgtggaaggc ggcttgccga tcccgttgac aactccggcg cacggcacgg
     5221 cgtatgacat cgtcggccag ggcaaggcga gcggcaccgc gatggcgcgc gccttcgatg
     5281 tcgcctgccg catggtccga tcccggcaga ccgccgccgt ctgaacgtcg cacgttctgt
     5341 tgtcccacaa cccatacaga ctggccgcgc gcgttgcgcg taagccggca atcttccgga
     5401 ggagacatga atccagcatt gacgcgttcc ccgcgtacgc acgttcagca gggcgtgctc
     5461 gcgctcctgt gtgcgatgtc attcatcctg tatctcgatc gggttaacct cgctgccgca
     5521 gctggaccca tcaaagccga actcggcctg tctaacacca cgctgggtgt cgcattctcg
     5581 gcgttcggct atacctatgc gatttttcag atcatcggcg gctggctcgc cgatcgcatg
     5641 ggcgcgcgcc gcacgttgct gatctgcggc tcgatatggg ttgtcgcgac aatcgcaacg
     5701 gggatggtcg gcggtcttgc atcgctgtgc gcagcgcgcc tgttgctcgg catcggcgaa
     5761 ggcgcggcgc tgcccgctca ggcgcgcgcc attgcgaact ggtttcatac gaaggaccgt
     5821 ggcttcgttc aaggcgtcac ccattcgtcg tcgcggctcg gcaatgccgc ggcgccgacg
     5881 ttagtcgtgt tgctcgcgac gctctattca tggcgtctgt cgttcatcct cgtcggcggc
     5941 ttcacggcgg tgtggatcgt cgtgtggatg gtgtattacc gggacgatcc gcgcgcacat
     6001 gggcgcgtca acgaagcaga actcgcatgt ctgcctgtcg gcatggtcca tgagtcgcag
     6061 gaacttcaag gcactccgtg gcgtcgcatc ctgcgccgca tgtcgccgac gatcttcgtg
     6121 tacttctgcc aggtgtggag caacacgctg tttttcagtt gggtgccgct cttcttcctg
     6181 cacggctacg gattgaatct caaggaatcc gccatatttt cttctggcgt gtttctcgcc
     6241 ggcgtggtcg gtgacgtcct tgggggcttc ctgagcgacc gggtgttaaa gcgtacgggc
     6301 aacctcaagc ttgcgcgtca gggggtgatt gcgttcagtc tgcttggtgc gttcgtcttc
     6361 atgattccgg tgatgatcag ccgggacctg acagtcgtga cgctgtgcct gtccggcgca
     6421 ttcttcatgc tcgaactgac tatcgggcca atctgggcag tgcctatgga tgtggcggaa
     6481 cgccatgcgg gcacggcgag cggcatgctc aatacgggtg ctgccttggc cacgatcatc
     6541 tcgcccgtgg tgttcggggc ggtgatcgat gcgaccggaa actggagcct gccgttcgtc
     6601 ggttccttgc tcttcctgct acttggggcg gtgatgacac taaagattcg gccagacctc
     6661 gacattggtg agggcctcgc tccgtcgggt aacgctgacc caatcccggc tcaacgtagt
     6721 cagcgaatga accaaggagg ggcggaaaaa agcatcacgg tcatggacgg agatgcgagc
     6781 taaataagat gtgtatttat cgcataactt aatcctgcgt tctccttaag gcgcaccacg
     6841 ctgagtccga ccgaaacccg caacccgttg cttaccaaac ctgttcgagg ggtgtcgtac
     6901 tagggcgcgt acttcggcaa cagcgctgtc agtgccgata cgcgctcctt cagcacttca
     6961 tgcagctgcc ggatcgcggg aaagaactgc ttgcgatgcg tgcagatcag gttcagcggc
     7021 gccgcctcgc cgggctgctc gggcatcagt atttcgagcc ggcccgtctg cacgtcggcg
     7081 cacacgtcga gccacgattt gtacgcaatc ccctggccct cgatcgccca gcgccgtgcc
     7141 acgtcggcgt cgtcgcaaaa gagcgacccg gcgacggcca cctggcggag ctcgcccccc
     7201 acggggaaca tccatttgtc gtacacgcgg ccgcccagga catagagcag gcagtggtgg
     7261 cctgccagtt cgtccagcgt gcgtggccgc ccatgccgtt gcagataggc gggcgacgcc
     7321 accagcacac gccgattgtc gctggccagc ggcagcgcga cgtagctcgc gttatcggcg
     7381 cggccgtagc ggatggcgat ctcgaccgga tcgcgaaaca tgtcggtcag ttgatcggac
     7441 aaaaagagcc gcaccgacag cttcggatgc gcctcgcgaa acgcagtgat ccagggcagc
     7501 agaacattgc ggcccagatc cgacggcgcg gcgatctgca aaggcccctg cagcgcgtcc
     7561 gccgcgccct gcaggcactc ccgcccctcc tgcaatgtcg ctagcacctg cttcgcgtac
     7621 ggcagatatt cctcgccttg ggccgtaagc cgcaggctgc gcgttgagcg cgcgaacaaa
     7681 cgaacgtcga gctcgcgctc gagccgctgg attgccgcgc tgacctggcc cggcagcagg
     7741 gccgcttcgc gggcggcttt cgagaagctg ccgagcgccg cggtgcgcac gaacagcgcc
     7801 aggtcgtcga accggatcat tttcattcct g
//
LOCUS       DATASH010000101         3239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5156491,
            whole genome shotgun sequence.
ACCESSION   DATASH010000101 DATASH010000000
VERSION     DATASH010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5156491"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /gene="yidC"
                     /locus_tag="VHV99_03110"
     CDS             complement(<1..1034)
                     /gene="yidC"
                     /locus_tag="VHV99_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEX3634473.1"
                     /translation="MDIKRTVLWVIFFMSAVMLFDNWQRDHGRPSMFFPSAVPTKTVG
                     SAAPGTTTPGTQAADLPATPGVPGAPVPPGSNAPASTAADQLVNFSTDVYSGQIDTRG
                     GTLSKLSLVKEGDGKQPDLYITLFDRTATHTYLARTGLLGGDFPNHNDVFTLVPGGQT
                     TMGDGNTLTISFESPVKGGVKVIKTYTFTRGSYVIGVDTKVQNVGAAPVTPRLYMELV
                     RDDSPVETPRFSHTFIGPAVYTEQHHFQKMTFSDIDKGKEDFATSADNGWVAMVQHYF
                     ASAWIPQQGAKRDIYVQKIDPALYRVGVQQPLQTIAPGQTVDVQSRLFAGPEEERMLE
                     GVAPGLELVK"
     gene            complement(1043..1423)
                     /gene="yidD"
                     /locus_tag="VHV99_03115"
     CDS             complement(1043..1423)
                     /gene="yidD"
                     /locus_tag="VHV99_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX3634474.1"
                     /translation="MAETALIALLRFYKVAVSPMLGNRCRFYPSCSDYAREAIQYHGA
                     ARGSFLAAKRLCRCHPFSAGGIDLVPVPPSAAGCVQQDGASAADPVDDSAPSSSNSPN
                     PSPDPANSVSSNPHGANNALFDRH"
     gene            complement(1492..1983)
                     /gene="rnpA"
                     /locus_tag="VHV99_03120"
     CDS             complement(1492..1983)
                     /gene="rnpA"
                     /locus_tag="VHV99_03120"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008352224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEX3634475.1"
                     /translation="MRSTTSSGQETSQLRASAAFPKAARLLKTDEYSSVFRLRPWRRS
                     PHFVLYGKPTGNEARLGLVIGKKFAPRAVTRNLIKRLAREAFRLRRAELGGWDILLRL
                     HAKIDRKAMPSAKSPPLRMLFREEIDGLLDRAVREAARRTEGVGEQGASPIVNDPPAP
                     AGS"
     gene            complement(2037..2171)
                     /gene="rpmH"
                     /locus_tag="VHV99_03125"
     CDS             complement(2037..2171)
                     /gene="rpmH"
                     /locus_tag="VHV99_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX3634476.1"
                     /translation="MKRTYQPSVTRRKRTHGFRVRMKTAGGRKVINARRAKGRKRLAV
                     "
     gene            2590..>3239
                     /locus_tag="VHV99_03130"
     CDS             2590..>3239
                     /locus_tag="VHV99_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA N-terminal domain-containing protein"
                     /protein_id="HEX3634477.1"
                     /translation="MNDFWQHCSALLERELTPQQYVTWIKPLALVDFDADASTLRIAA
                     PNRFKLDWVKSQFSGRIADMAREFWHVPVDIQFVLDPKATARKAMGGAPAPYAASTFS
                     SAPAGTPTSPPMPRPAPQHAERNNGGHANASAHINALAAEAAQHAQSLQHNDQADNGD
                     LDLPSLDANEAAAARRTWRPGGTASSNGENDSAYERSKLNPVLTFDNFVTGKANQLA"
BASE COUNT          620 a         1011 c         1009 g          599 t
ORIGIN      
        1 tccttcacga gctcaagacc cggcgcgacg ccttccagca tccgctcctc ttccggaccg
       61 gcaaaaagcc gcgattgcac gtcaaccgtc tgccctggcg caatagtctg caacggctgc
      121 tgcacgccga cgcgatacag cgcgggatcg atcttctgca cgtagatatc gcgcttcgcg
      181 ccctgctgcg gaatccacgc cgacgcaaaa taatgctgca ccatcgcgac ccagccgtta
      241 tccgcagaag tcgcgaaatc ttccttgccc ttgtcgatat cgctgaacgt catcttctgg
      301 aaatgatgct gctcggtata gacagccggc ccaatgaacg tatgcgagaa gcgcggcgtt
      361 tccaccggcg aatcgtcgcg cacgagttcc atatagaggc gcggtgttac cggcgcagcg
      421 ccgacgttct gcacctttgt gtcgacaccg atcacatagc tgccgcgcgt gaacgtgtag
      481 gtcttgatga ccttcacgcc acccttgacc ggcgactcga acgagatggt caacgtgttg
      541 ccgtcaccca tcgtcgtctg gccgcccggt acgagcgtga agacgtcgtt gtggttcggg
      601 aagtcgccgc cgagcaggcc cgtgcgcgcg agatacgtgt gcgtcgcggt gcgatcgaag
      661 agcgtgatgt acagatccgg ctgcttgccg tcaccttcct tcacgagcga cagctttgaa
      721 agcgtgccgc cgcgagtatc gatctggccg gagtagacgt ccgtggagaa attcacgagc
      781 tggtctgcgg cggtcgatgc gggtgcattc gaaccaggcg ggaccggtgc accgggtacg
      841 ccaggcgtgg ctggcaaatc ggcggcttgc gtgcccggcg tcgtggttcc cggtgcagcg
      901 ctgccgaccg tcttggtcgg gacggcgctc gggaagaaca tcgacgggcg cccatggtca
      961 cgttgccagt tgtcgaacag catgacagcc gacatgaaaa agatgaccca taggacggta
     1021 cgtttgatat ccatgcgttg tctcagtgtc gatcgaagag cgcgttattg gcgccgtggg
     1081 ggtttgaact tactgaattt gccggatccg gcgaaggatt tggcgaattc gatgagctcg
     1141 gggcagagtc gtcgactggg tcggccgctg acgcgccatc ctgctgaacg cagccggcgg
     1201 cggacggagg cacggggacc agatcgatgc cgccagccga aaacggatgg cagcggcaca
     1261 gacgttttgc tgcgaggaat gagccacgcg cggcgccatg atactggatt gcttcgcgcg
     1321 cgtagtcaga acacgatggg taaaagcggc accggttgcc gagcatgggg ctcaccgcaa
     1381 ccttgtagaa gcgtaataag gcgatcaacg ccgtttctgc cattcgtgcc gtctgcatag
     1441 aattcgcgct cgggtcgacg ccggaccaag ccggtgtgcg cctgaagcgg ctcatgagcc
     1501 cgcaggcgcg ggtgggtcgt tgacaatggg cgatgcaccc tgctcaccca cgccttcagt
     1561 acgtcgagcg gcttcgcgca ctgcacggtc gagcaggccg tcgatctctt cgcgaaacaa
     1621 catgcgcagc ggcggcgatt tcgcgctcgg catggccttg cggtcaattt tcgcatgcag
     1681 ccgcagcagg atgtcccagc caccaagctc tgcacgccga agccggaatg cctcgcgcgc
     1741 caaccgcttg atcaggttgc gcgtcactgc acgcggcgca aacttcttgc cgataaccag
     1801 acccagtcgc gcctcgttgc cggtcggctt gccatacagg acaaaatgcg gagaccggcg
     1861 ccaggggcgc aaacgaaaaa cggatgagta ttcatccgtt ttcagcagcc ttgcggcctt
     1921 ggggaacgcg gcgctcgctc gcaactgact ggtttcctgt ccggagctgg tcgtgctgcg
     1981 cacctggccg tcgcacgcgt cggggcgagt gccggggcgc gccgtcaacc gcgaccttat
     2041 acagccaggc gcttgcggcc cttcgcgcga cgtgcgttga tcaccttgcg gccgccggcc
     2101 gtcttcatac ggacgcgaaa gccatgggta cgcttgcggc gggtaacgga aggttggtaa
     2161 gtgcgtttca tgttggtctc acttgggtaa aaacggaccg ccggccatac gtaagcgaca
     2221 aaaaggcgac gcaccggccg gtggtacagg aattcgggtt ttgcggaacc cgctatttaa
     2281 accgttttcc tattgaccgt caataggtta ccaggcatgg cgagcggttt tccgagaagt
     2341 ccacaggccc gatgattcga tacacgacct ggtccgtccc tgtggataac tcgcgccgcg
     2401 gttgcatttg gcgttagaat ctccgctcaa tccgacgtaa ccgcacgttg tcccggtccg
     2461 ctgtaccgcc tgcaaaagca tggtggacaa ggcttcgggg cacgcttgcc cgcgcgcacc
     2521 gggccttggc gccgagtggc ggcaggggtg gcggcaggac cttgcggtaa accctaacga
     2581 ctgcattcga tgaacgattt ttggcaacac tgttccgcat tgctggagcg cgaacttacg
     2641 ccgcagcagt acgtcacatg gatcaaaccg ctggccctgg tcgacttcga tgccgatgcg
     2701 agcacgctgc gcatcgccgc gcctaatcgg ttcaagctcg attgggtgaa aagtcagttt
     2761 tccggccgta tcgccgatat ggcccgggaa ttctggcacg tgccggtcga tatccagttt
     2821 gttctggacc caaaagcgac cgcgcgcaag gcgatgggcg gcgcgcccgc gccgtacgct
     2881 gcatcgacct tttcatcggc gcccgctggc acgcccactt ccccgcccat gccgcggccg
     2941 gcgccccagc acgctgaaag aaacaacggc ggccacgcga acgcgagcgc ccacatcaac
     3001 gcgctcgcgg cagaagccgc gcagcacgcg caatccctgc aacataacga tcaggccgac
     3061 aacggcgatc tcgacctgcc gagcctcgac gccaatgaag ctgccgcggc gcgccggaca
     3121 tggcggccgg gcggcacggc gtcgtcgaat ggggaaaacg attccgctta cgagcgttcg
     3181 aagctcaacc ccgtcctcac tttcgacaac ttcgtgaccg gcaaggcgaa tcagctcgc
//
LOCUS       DATASH010000102         9682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1003600,
            whole genome shotgun sequence.
ACCESSION   DATASH010000102 DATASH010000000
VERSION     DATASH010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 9682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9682
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1003600"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..553)
                     /locus_tag="VHV99_03135"
     CDS             complement(11..553)
                     /locus_tag="VHV99_03135"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX3634478.1"
                     /translation="MTNENQLERWLAREQEVLAALNAGPGPGVASPEQVAGKTGLDVM
                     QAMMNAELPYAAIARTLDFTIVEVSAGHAVFQGTPLAQHLNPLGTVHGGWIATLLDSA
                     LGCSVHTMMPASRGYTTAELSVNYVKAVTPRVQRVRAEGKVIHCGRQLATAEARLVGP
                     DGTLYAHATTTCLVFEIPGR"
     gene            1147..3699
                     /gene="mprF"
                     /locus_tag="VHV99_03140"
     CDS             1147..3699
                     /gene="mprF"
                     /locus_tag="VHV99_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEX3634479.1"
                     /translation="MFDRVSKALGKRSILSPVLALVICGLLLVVLQHLSQAVDYRSVI
                     GQLRHLTAGEWTAALGATALSYIALVGRDAVGLRYLGATVPRAALWIGATAGSALGNA
                     TGFGALTGGAVRARVYAVSNVTPAQIGRMTVFTSVSLALALVLMTALGMVCLADPLAA
                     MLHLPPLTLRWCGAALLIVLGLAAAACRRDTRPVRTRWEWLSCDIPARRDLLAQVALA
                     VLDVVAAGLALWALLPHADVSFVTFITVYAAAMLLGMIGHTPGGVGVFEAAMVFTLNG
                     SVQTHQMLAALLAYRAIYFGVPLIVSAALLAGFEGRALKSRLPLRHAAGAYKLAPLFL
                     SLVTFVVGGMLVISSATPAFWQRILILRDVLPLWVLESSQMLCSVLGVLLLFVARGLL
                     RRLDAAWWMTLLLAVLSLALSLTKGLAFVEAGVLGMLIALLLSTRRRFNRHSSLFAER
                     FTAGWLVSVAMVLALAVWVMLFAFRDVPYTRDLWWQFAFDERAPRALRATLAASLFAA
                     IFSFWQLLRPAAGRFVIPPPQDLQDAARIVRAQERSDAGLALMGDKSFLFSESREAFL
                     MYAKNGRTWAALHDPVGPREEWAGLISKFVALAHAHGGRAAFYQVRANALPLYLDAGL
                     TLMKLGEEANVVLDDFDLKGSHRAHLRYALKRGERDGFTVEVIDQAAVPASLETLREI
                     SDGWLDSRDAREKSFSVAAFTDEYLAAQSVMLVRQNGEPAAFVTFMTTDMNTEATVGV
                     MRHVESASPYAMEYLFTQLALHLKQAGFRSLSLGIAPLSGMQPTPLASRWHRLAGIVW
                     RFGGRFYNFRGLRAFKSKFQPHWEPRYLAASGSMGVFFTLADLSLLAGGRRS"
     gene            3696..4961
                     /locus_tag="VHV99_03145"
     CDS             3696..4961
                     /locus_tag="VHV99_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcvB/VirJ family lysyl-phosphatidylglycerol
                     hydrolase"
                     /protein_id="HEX3634480.1"
                     /translation="MTLHPLFKLAVAASLVTGSAAAFAATTTVSGGRYGDVTVTQPAG
                     PLRGFVVLYSPAAGWSAADQQTADALAKAGALTVGVDTARYAANLGAKKEACHQLVGD
                     AEALSHQLERQSQSSRYFAPIVAGTGQGATLAMHVLEQAPSNTVAGAVSVDAERNLDA
                     RFQPCPPDPTIIRDKVPGFVEKATPGTGDRARLVAMVTPHLQTASTNDDDVSDLPLIE
                     LPAAHPNGLMAIVISGDGGWRDLDKTISLALQKDGVSVIGVDSLRYFWSEKTPAQTSH
                     DLARVMQTYGARWHAEHIALVGYSFGADVMPFAYNRLPEALRAKVSLIALLGFAPDAD
                     FQIRVGGWLGMPASDKALKVQPELTRVPPALVQCFYGENEKDTLCPALTRTGITVIRT
                     SGDHHFGGDYNALEQRILGAFRKASGVHD"
     gene            5318..6955
                     /locus_tag="VHV99_03150"
     CDS             5318..6955
                     /locus_tag="VHV99_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEX3634481.1"
                     /translation="MLDSFFFETAASEPDSPALWVDERLYCYDEIASRARRIAAEIAA
                     AMPPGQLGRCLLFAHRSEAAYVGLLGILKAGFAYVPLNPLVPTARIAAVIEQSGAPLL
                     LVDRQCAALLDEVLALLDDCPRILLLDDDDWAGAGDGQGAQDTQEMNATRTASAFGDG
                     LKRLPSEPGVTESAISRAPHDLAYILFTSGSTGAPKGVPISHANATAYVAGQLHTLGR
                     LSGARYAQLCELTFDPSVHDMFVCWANGGCLYVPKTVEPIYNAAFIKQHAITHWSSVP
                     SVAGFMQQFRKLGQNEFPSLRVTIFGGEPLPRLLVQAWRRAAPNSRVLNMYGPTETTV
                     ACAAFEVTAEFLDDPQHAVMPLGGALPGMELLIVDAALKPVVSGASGELLIGGPQVAA
                     GYLSADEPGNRRFVSTTYPGYSASRWYRSSDAAREAAGQGIVFQGRLDTQVKIRGNRI
                     ELEEVEHVVQACSHTALCAVLAWPVDEAGQACGLIAFVTNAQGGAVQILQACRQRLPA
                     YAVPQRIVTLDVLPLNVNGKIDRKALAERCASGDVVT"
     gene            7133..7393
                     /locus_tag="VHV99_03155"
                     /pseudo
     CDS             7133..7393
                     /locus_tag="VHV99_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181766.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
     gene            7416..9233
                     /locus_tag="VHV99_03160"
     CDS             7416..9233
                     /locus_tag="VHV99_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX3634482.1"
                     /translation="MTTLPTMRPVSFDGCLGWLHRPSGAQSAMGVVLCNPFGYEALCI
                     HRGWREFAEALAVRGMPALRFDYPGTGDSSGSEEDPQRLRAWLDSIKAAAAWLRAETG
                     VTRLSFCGLRLGATLAALAAEELGGVENLVLLAPVSSGKSYIRELEMQHQSWLSAQIG
                     LESSPDAEAGRTVGAHGFRLYPDTLESLAHVDLEQRTQRPARRVLVQDLAESARVKRL
                     VARYQVQGAQADLQVFSEYGKFLLDPRYSVPPRHGFDGVLEWLGASALSAGEPAAAIV
                     DPGGAARIDFADGCETPVVFGGGRYVGIFCQPRGALDGAPAVLFVNTGGVHRVGDSRI
                     AVLMARRLAARGIASLRMDLGGIGDSVRRDDSLTLDVVYARHAVTDAKAGVDCLAAAG
                     HPKTVMFGVCTGAYVSMHTALAHAGVIGCALVNLPFFEWGGARARLSTRHVASSGVYR
                     LSVRNPRKWLRLLTGRANGRAIVTELARRLCARISVRVSTAIEHLLGRPTAGGAIRRL
                     ALELERKGVQTSLMYGPLDEGLDELAIHFGPNGNQLAKLKNVNVKVVGKVDHALFSHA
                     ARDAVMAQFDLFLRERVLGARHEAAASKSGLRVAQARQS"
     gene            9486..>9682
                     /locus_tag="VHV99_03165"
     CDS             9486..>9682
                     /locus_tag="VHV99_03165"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089585.1"
                     /note="threonine deaminase; threonine dehydratase; in
                     Escherichia coli, IlvA is part of the isoleucine
                     biosynthetic pathway; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HEX3634483.1"
                     /translation="MPLHELKRAAVAADSVALRHDYLKKTLTARVYDVARETELERAP
                     NLSARLRNPVYLKREDNQPVFS"
BASE COUNT         1561 a         3033 c         3159 g         1929 t
ORIGIN      
        1 accgtatcct ttagcggccc ggaatctcga acaccagaca ggtcgtcgtg gcatgcgcgt
       61 agagcgtgcc gtcggggccg acaaggcgag cttccgccgt cgccagctgc cgtccgcagt
      121 ggatcacctt gccttcggcg cgcacgcgct gcacgcgcgg cgtcaccgct ttcacgtaat
      181 tcacgcttag ctcggcggtg gtatagccgc ggcttgccgg catcatcgtg tgtaccgagc
      241 agcctagcgc ggaatcgagc aaggtagcga tccagccgcc atgcactgtg cccagtggat
      301 tcagatgctg ggcgagcggc gtgccctgaa acaccgcgtg ccccgcactg acttcgacga
      361 tggtgaagtc gagtgtgcgt gcgatcgcgg cgtagggcag ttccgcgttc atcatcgcct
      421 gcatcacgtc caggccagtc ttgcccgcca cctgttcagg gctggcgaca ccggggccgg
      481 gcccggcgtt cagtgccgcc aatacttcct gctcgcgcgc gagccagcgc tcgagttgat
      541 tttcgttcgt caatccgtgt ctcctgtcct gaggcaagcg tgcctggaaa tcgatgcgtc
      601 gtcatcgatt agagcatcaa accgattctg ggcgcctcga gggcggcctc tggaccgccg
      661 gcacgcgtag cgccgtctcg ttcacaataa gactcggatt gcccgtgcgt cacttgcgga
      721 tatgcgacgc ctcccgccgc gccaccttgg tcgctcgcgc ggacaacgcg ctcgtcagaa
      781 gcgccgtcga caccaacccg atcaaatatc tccatctcgc gatctgccat aagcgtttag
      841 acttcgcccc cagtcgagcg cagcggtccg cacccagcac aacagtccaa tcggcaatat
      901 cgaccgtcta atccacgcgg attgctctgt cacgccgcga agcgcattag tttcaatcgc
      961 ggcccaggtt aaaacccgat gaattgactg acactgtcaa ccttttccac atattgccgt
     1021 tatctattcg taatcgctga agtaaggcga tgccgtggca gacgatccgt cgccgcgaca
     1081 gctcccgcaa ggcccgcatt gaccatgttc caccgataca acaataaatt cgaccggcca
     1141 ggcgcgatgt tcgaccgtgt atcgaaagcg cttggtaaac ggagcatcct ctcgcccgtg
     1201 ctcgcactcg tcatttgcgg gttgctgctg gtcgtcttgc aacatctctc gcaagcggtc
     1261 gactatcgct cggtcatcgg ccaactgcgg cacctgacgg cgggcgagtg gaccgcggcg
     1321 ctcggcgcga ccgcgctcag ctatatcgcg ctggtcggcc gcgatgcagt cggcttgcgc
     1381 tatctgggcg ccacggtgcc gcgcgcggcg ctatggatcg gcgcgaccgc gggctccgcg
     1441 ctcggcaacg cgaccggctt cggcgcgttg acgggcggcg ccgtgcgcgc gcgcgtctac
     1501 gccgtctcaa acgtcacgcc cgcacagatc ggccgcatga ccgtgttcac gagcgtttca
     1561 ctggcgctcg cgctggtgct gatgaccgcc ctcggcatgg tgtgcctcgc cgacccgctc
     1621 gcagcgatgc tgcatctgcc gccgctcacg ttgcgctggt gcggcgcggc tttgctgatc
     1681 gtgctcggac tcgcggctgc cgcttgccgc cgcgacacgc gcccggttcg cacgcgctgg
     1741 gaatggctct cgtgcgatat tccggcgcgc cgcgacctgc tcgcgcaagt ggccctcgcc
     1801 gtactcgacg tggtggccgc gggcctcgcg ttatgggcgc tgctgccgca tgcggacgtc
     1861 agcttcgtga ccttcatcac ggtctatgcc gccgccatgc tgctcggcat gatcggccac
     1921 acgcccggcg gtgtcggggt gttcgaagcg gccatggtgt tcacgctcaa cggcagtgtg
     1981 caaacgcatc agatgcttgc ggcgctgctc gcttatcgcg cgatttactt cggtgtcccg
     2041 ttgatcgttt cggcggcgct gctggccggc ttcgaaggcc gcgcgttgaa aagccgtttg
     2101 ccgctgcggc acgcggcagg tgcctataaa ctcgcgccgc tgtttctgag cctcgtgact
     2161 ttcgtggtcg gcggcatgct ggtgatttcc agcgcgacgc ccgcattctg gcaacgcatt
     2221 ctgattttgc gcgacgtgct gccgctgtgg gtgctcgaaa gctcgcagat gctgtgcagc
     2281 gtgctcggcg tgctgctgtt gttcgtcgcg cgcggcttgc tgcggcgcct ggatgcggca
     2341 tggtggatga ccttgctgct ggcggtgctg agcctcgcgc tgtcgctgac caagggtctc
     2401 gccttcgtgg aagcgggcgt gctcggcatg ctgattgcac tgctgctgtc cacgcgccgc
     2461 cgtttcaacc gtcactcgtc gctgtttgcc gagcgtttta cggcgggctg gctggtctcg
     2521 gtggcgatgg ttctcgcgct cgccgtatgg gtcatgctgt ttgcgttccg cgacgtgccg
     2581 tacacgcgcg acctgtggtg gcaattcgct ttcgacgaac gcgctccgcg tgcgttgcgc
     2641 gcgacgctgg ccgcgagcct gttcgcggcg attttctcgt tctggcagtt gctgcgcccc
     2701 gcggcgggcc gcttcgtgat cccccctccg caagatctgc aagatgcggc gcgtatcgtg
     2761 cgcgcgcagg agcgcagcga cgccggtctc gcgctgatgg gcgacaagag cttcctgttt
     2821 tccgagtcgc gcgaggcctt cctgatgtat gcgaaaaatg gccgcacatg ggccgcgctg
     2881 cacgatccgg tcggtccgcg cgaagaatgg gccggcttga tcagcaagtt cgtcgcgctt
     2941 gcgcatgcgc acggcggtcg cgccgcattc tatcaggtgc gcgccaatgc cttgccgctc
     3001 tatctcgacg cgggcctcac gctgatgaag cttggcgagg aagccaatgt cgtgctcgac
     3061 gatttcgatc tgaagggctc gcatcgcgcg catcttcgct atgcgttgaa gcgtggcgag
     3121 cgtgacggct ttaccgtcga agtgatcgat caggccgctg tgccggcctc gctcgaaacg
     3181 ctgcgtgaaa tttccgacgg ctggctcgac agccgcgatg cgcgcgaaaa gagcttttcg
     3241 gtggccgcgt ttaccgacga gtatctcgcc gcgcaatccg tgatgctggt gcgccagaac
     3301 ggtgagccgg ccgctttcgt gacctttatg acgaccgata tgaacaccga ggcaactgtc
     3361 ggcgtgatgc ggcacgtgga aagcgcgtcg ccgtatgcga tggaatatct gttcacccaa
     3421 ctcgcgctgc atctgaagca ggcgggtttt cgctcgctca gtctgggcat cgcgccgctc
     3481 tccggcatgc agccgacgcc gctcgcgtcg cgctggcatc ggctggcggg gatcgtgtgg
     3541 cgcttcggcg gccgcttcta taacttccgc ggactacgcg ctttcaagag caagttccag
     3601 ccgcattggg agccgcgcta tctcgcggcg tcgggttcga tgggcgtgtt cttcacgctc
     3661 gcggacctgt cattgctggc gggaggccgg cgttcatgac attgcatccg cttttcaagc
     3721 tcgccgtcgc ggcgagcctc gtgactggca gcgccgctgc ttttgccgcg actactaccg
     3781 tatcgggcgg ccgttatggc gacgtgacgg tgacgcaacc cgccggcccg ttgcgcggct
     3841 tcgtggtgct ttactcgccg gcggccggct ggagtgcagc cgaccagcaa accgccgatg
     3901 cgctcgccaa ggcaggcgcg ttgaccgtcg gcgtggatac ggcgcgctat gcggccaatc
     3961 tgggcgcgaa aaaagaagcc tgccatcaac tggtgggcga tgctgaagcc ttgagtcatc
     4021 aactcgaacg gcaatcgcaa tcgagccgct atttcgcacc gatcgtcgcg ggtacgggcc
     4081 agggcgcgac gctcgcgatg catgtgctcg aacaggcgcc gtcgaatacg gtggccggcg
     4141 cggtctcggt ggatgccgag cgcaatctgg atgcgcgttt tcaaccgtgc ccgccggatc
     4201 cgacgatcat ccgcgacaag gtgccgggct tcgtcgaaaa ggcgacgccc ggcaccggcg
     4261 atcgcgcgcg gctcgttgcg atggtcacgc cgcatttgca gacggcgtcg acgaacgatg
     4321 acgatgtctc cgatctgccg ctgatcgaat tgccggcggc gcatccgaac ggcctgatgg
     4381 caatcgtgat ttccggcgac ggcggctggc gcgatctcga caagaccatc tcgctcgcgt
     4441 tgcagaaaga cggcgtctcg gtgatcggcg tcgacagcct gcgttatttc tggagcgaaa
     4501 agaccccggc gcagacgagc cacgatctcg cccgcgtgat gcagacctac ggcgcgcgct
     4561 ggcatgcgga gcacattgcg ctcgtcggct attcgttcgg cgccgacgtg atgccgttcg
     4621 cctataaccg tttgcctgaa gcgctgcgcg cgaaagtgtc gctgattgcg ctgctcggct
     4681 tcgcgcccga cgccgatttc cagattcgtg tgggcgggtg gcttggcatg ccggcgagcg
     4741 ataaggcctt gaaggtgcag ccggaattga cgcgcgtccc gcccgcgctc gtgcagtgtt
     4801 tctacgggga gaacgagaag gacaccttgt gtccggcgct taccaggacc ggcatcacgg
     4861 tgattcgcac gtccggcgat catcacttcg gcggcgacta caatgcgctg gagcagcgca
     4921 ttctcggcgc tttcaggaag gcaagcggcg tgcatgattg atacgccgca tggcgtgtga
     4981 cgcgaagggc cacatcatcc cgcgcgcggc gggcggcccg gcgtctcttc attcgtctgt
     5041 tgagagtggt atcgcacata tatcggtccc actggtctct atgattatta cgaagacctg
     5101 acgacagtct cctcatacgt tttgcgtgct gaggccgttt tgctggcgtg tcgtccgtga
     5161 atctctcttg tgaaattttc ctgcgataac gcagcgcgtc gccgggcagc tttgctgtct
     5221 ggtcgcgtgc tcccggcatg actggcaact gctcgcgcgc ccgcctgggg ctcgccgatg
     5281 cgggtccggt gcgatgcccc atggaccgag gccgtgaatg ctggattcct tcttcttcga
     5341 aactgcggcg agcgagccgg acagtcccgc gctatgggtc gatgaacgac tctattgcta
     5401 tgacgagatc gcctcgcggg cgcgccggat cgccgccgag atagcggccg caatgccgcc
     5461 cggccagctc gggcgctgtc tcctgttcgc gcatcgcagc gaggctgcct atgtggggct
     5521 gctggggatt ctcaaggccg gcttcgccta tgtgccgctc aatccgctcg tgcccaccgc
     5581 gcgcatcgcc gccgtgatcg agcagtcggg cgcgccgctg ttgctggtcg atcggcagtg
     5641 tgccgcgttg ctcgacgaag tgctggcgtt gcttgatgac tgtccacgga ttctgctgct
     5701 cgatgatgac gattgggcgg gcgcaggaga tggtcagggc gctcaagaca ctcaagagat
     5761 gaacgccacg cgcactgctt cagccttcgg cgacggcttg aagagactgc cttccgagcc
     5821 aggcgtgaca gaatccgcca tttcccgcgc accgcacgat ctcgcttaca tcctctttac
     5881 gtcgggttcg accggtgcgc ccaaaggcgt gccgatctcg cacgcgaacg ctaccgccta
     5941 cgtggcgggg caactgcaca cgctaggcag gctgtctggc gcgcgctatg cgcaactgtg
     6001 cgagctcact ttcgatccgt cggtacatga catgttcgtg tgctgggcaa acggcggctg
     6061 cctgtatgta ccgaagaccg tcgagccgat ctacaacgcg gccttcatca agcagcacgc
     6121 gatcacgcat tggagcagcg tgccctcggt agccggtttc atgcagcagt ttcgcaaact
     6181 ggggcagaac gaatttccga gcctgcgcgt gacgatcttc ggcggcgagc cgttgccgcg
     6241 tttgctggtc caggcgtggc ggcgcgccgc gccgaacagc cgggtgctga acatgtacgg
     6301 gccgaccgaa accacggtgg cctgcgcggc attcgaagtc acggcggaat ttctcgacga
     6361 tccacagcat gcggtcatgc cgctcggtgg tgcattgccg gggatggaac tgttgatcgt
     6421 cgacgccgcg ttaaagccgg tcgtgtccgg cgcaagcggc gaattgctga tcggcggccc
     6481 ccaggtggcc gcgggctatc tgtcggcgga cgaacccggc aatcgacgct tcgtctcgac
     6541 aacgtatcca ggctacagcg cgtcgcgctg gtaccgcagc tcggacgccg cgcgcgaagc
     6601 agccgggcag ggcatcgtgt ttcaagggcg gctggacacg caggtcaaaa ttcgcggcaa
     6661 ccggatcgaa ctcgaagaag tcgagcacgt cgtgcaggcc tgcagccaca cggcgctctg
     6721 cgcggtgctc gcgtggccgg tcgacgaagc gggccaggct tgcggcctga ttgccttcgt
     6781 gacgaacgca cagggtggcg ctgtgcagat tctgcaggca tgccgtcagc ggctgcccgc
     6841 gtacgccgtg ccgcaacgga tcgtgacgct cgacgtgttg ccgctcaacg tgaacggcaa
     6901 gattgacagg aaggcgctcg ccgaacgctg tgcaagcggc gacgtggtca cctgacaggc
     6961 cgcgcgacac tatgcttcgg atggcttaag agtttatcga ggtgctgaaa atttttcaaa
     7021 gtagggacta tctgaaatcg ggccaagtac aggctaagta caagcggtac atccttgcat
     7081 gttctcgatg catcgatgtc ttgcgtaaaa attcaggtat cgggggtatc aaatgaaaga
     7141 tcatgtgcgc agcattcttc gggagaccgc ctgtctcgac gtgccggtcg acacgctcgg
     7201 cgaccgcgac gatctctacg cggcgggcct atagtcgctc ggctcggtcc gcgtgatgat
     7261 ggccattgaa gagaacttcg ttatcgaaat tccaggcgtg ctgattacgc acgatctgtt
     7321 tcagagcatc gattcgctcg caagcacgat tgcgcgaatc gtgccggaag agtcgctcgc
     7381 gtcgcgaact tgacgtggcg tcgccgcgac cgatcatgac gacccttccg accatgaggc
     7441 cggtaagctt cgacggctgt ctgggatggc tgcaccggcc gtcgggcgct cagtccgcaa
     7501 tgggggtggt gctgtgcaac ccgttcggct acgaggcgct gtgcattcat cgcggctggc
     7561 gcgagttcgc cgaggcgctc gccgtgcgcg gcatgcctgc cttgcgcttc gactatcccg
     7621 gcaccggcga ctcgagcggc agcgaagaag atccccagcg cctgcgcgca tggctcgaca
     7681 gcatcaaggc ggccgcggcg tggctgcgtg ccgaaaccgg tgtgacgcgc ctgagtttct
     7741 gcggactgcg tctcggcgcc acactcgcgg cgctggccgc cgaagagctc ggcggtgtcg
     7801 aaaatctcgt gctgctggcg ccggtgagtt cgggcaagag ctacatccgt gagctggaga
     7861 tgcagcatca aagctggctc agtgcgcaaa tcggacttga aagcagcccc gacgccgagg
     7921 cgggacgcac ggtcggcgca cacggattcc gcctctatcc cgacacgctc gagtctctcg
     7981 cgcacgtcga tctcgaacag cgtacgcaac gtcctgcgcg gcgcgtgcta gtgcaggacc
     8041 tggctgagag cgcgcgggtg aaacgcctgg tcgcgcgtta tcaggttcag ggcgcgcagg
     8101 cggatctgca ggtgttcagc gaatacggca agttcctgct tgatcctcgc tacagcgtgc
     8161 cgccgcgcca cggcttcgac ggcgtgctgg aatggctggg cgcaagcgcg ctcagcgcgg
     8221 gtgagcccgc ggcggcgata gtcgatccgg gcggagccgc gcggatcgat ttcgcagacg
     8281 gctgcgaaac gcccgtggtg ttcggcggcg gccgctatgt cggcatattc tgccagccgc
     8341 ggggcgcgct cgacggtgcg cctgctgtgt tgttcgtcaa cacgggcggc gtacaccggg
     8401 ttggcgatag ccggattgcc gtgctgatgg cgcggcgcct cgcggcacgt gggatcgcgt
     8461 cgttgcggat ggacctcggc ggtattggcg atagcgttcg ccgtgacgac tcgctcacgc
     8521 tcgatgtggt gtacgcccgg cacgcggtga cggacgccaa agccggggtc gattgcctcg
     8581 cggcggcggg ccatccgaag acggtcatgt tcggcgtatg caccggcgct tatgtgagca
     8641 tgcatacggc gctcgcgcat gctggcgtga ttggctgcgc gttagtcaat ctgccgtttt
     8701 tcgagtgggg cggcgcacga gccaggctta gtacccggca cgttgcgtcg agcggcgttt
     8761 atcggctttc cgtgcgcaat ccgcgcaaat ggctgcgcct cctcaccggc cgcgcgaacg
     8821 gccgcgccat cgtgaccgaa ctcgcgcgac gtctctgcgc gcgcatcagc gtgcgtgtca
     8881 gtaccgcgat cgaacatctg ctcggccggc cgacggcggg tggcgcgatc cgcagactcg
     8941 cgctggaact ggagcgcaag ggcgtacaaa cctcattgat gtatggccca ctcgacgagg
     9001 gtctggacga actggcgatt cattttggac cgaacggcaa ccagcttgcc aagctgaaga
     9061 acgtcaacgt gaaagtggtc ggcaaggtcg accatgcgct tttttcgcat gctgcgcgcg
     9121 acgcggtgat ggcgcagttc gatctgtttt tgcgcgaaag agtgctcggt gcgcgacatg
     9181 aagctgctgc gtcgaagtcc ggcttgcgcg tcgcgcaggc gcggcagtct tgagtgttcg
     9241 tgctgtttgc ggcgcacgga caagggattg acaaagcccg tcaagtcaac gaagatcgaa
     9301 cgcatgaact gcctcgacat ccgtattgcg ctgattatta gcaccattca tcgaatggtg
     9361 ggttagcgcg cgtctgatag cagtgacatc aacccgcccc acgaggcggg tttttttatg
     9421 tccgctttct gcctgcctcc tggtgacttt cttgttgact tgaccttgcc gcacaggagt
     9481 ttgccttgcc gttacatgaa ctcaaacggg cggccgttgc cgccgatagc gtggcattgc
     9541 gccacgacta tctgaagaaa accctgaccg cgcgcgtcta cgacgtggcc cgcgagaccg
     9601 aactcgaacg cgcgccgaat ctgtcggcac ggctgcgtaa tccggtttat ctgaagcgcg
     9661 aggacaacca gccggtgttc tc
//
LOCUS       DATASH010000103         1608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1794115,
            whole genome shotgun sequence.
ACCESSION   DATASH010000103 DATASH010000000
VERSION     DATASH010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1794115"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..176
                     /locus_tag="VHV99_03170"
     CDS             15..176
                     /locus_tag="VHV99_03170"
                     /inference="COORDINATES: protein motif:HMM:NF023203.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634484.1"
                     /translation="MTSSGIELIGIGLLDDAVEMFYAHNIVVRKLDELPTMAFNVLSQ
                     LLLKRGTRR"
     gene            279..800
                     /locus_tag="VHV99_03175"
     CDS             279..800
                     /locus_tag="VHV99_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX3634485.1"
                     /translation="MPAIDLLRQSVSSALVIAARQWRRTSQGVIAAYNVSEACATPLL
                     IAARLDAPVRQVALAELTGIEGPSLVRLLDQLCAANLVRRDEDPDDRRAKIISLTDEG
                     RLVTAKIEKELIGLRAGVLNDVSREDLEATLRVFAAFKLGASSGQAQQVPTPASHQGP
                     VIGPTDTDTEEGV"
     gene            807..>1608
                     /locus_tag="VHV99_03180"
     CDS             807..>1608
                     /locus_tag="VHV99_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEX3634486.1"
                     /translation="MTYPTSREWIFSVKTFAASMLALYIGLKLELPRPYWAMATVYIV
                     SNPFVGATRSKALYRALGTMIGASAAVLFVPPFVESPYLFSAVVALWTGTLLLLAISE
                     RTARSYVFMLAAYTMPLIALPSVMDPAGVFDLAVSRTEEIVLGIVCASVVGGAILPSR
                     LAPTLIERTDAWFRDAAFYAKETLSGRTSGTAISACRQRLAVTVNALEFLLSQLAYDH
                     TRPDVLARTQALRGRMQLLMPIMSAISDPLAALFNGQNPPPEGLEPLLA"
BASE COUNT          287 a          533 c          479 g          309 t
ORIGIN      
        1 cgcgcatcga cgagatcacc agttcgggga tagaactgat cggcatcgga cttctcgacg
       61 acgccgtaga aatgttttat gcgcataaca ttgttgtgcg caaacttgat gaactcccca
      121 cgatggcgtt caacgtcctg agccagctct tgctcaagcg cggcaccagg cgttaggccg
      181 ctctcttatc ggcccctcgc gtggttcaaa agcatcccgc cccaaaaaat caattcaata
      241 gcttgctaac gaattgttgt cgaaatacaa tgcgcgccat gccagcaatc gatctccttc
      301 gtcaatccgt cagcagcgcg ctcgtcattg cggcgcgcca gtggcggcgc acaagccaag
      361 gtgtgatcgc agcgtacaac gtctccgaag cctgcgcgac gccgctgctg atcgcggcgc
      421 ggctcgacgc acccgtgcgg caagtggcgc tcgctgaatt gactggcatc gaagggccgt
      481 cgctggtgcg cctgctcgat cagctgtgcg cggccaacct cgtgcggcgc gacgaagacc
      541 ctgacgaccg gcgcgcgaaa atcatctcgc tcaccgacga aggccggttg gtcaccgcca
      601 agatcgaaaa agaactgatc ggactgcgtg ccggcgtgct gaacgatgtc agccgggaag
      661 acctggaagc gaccctgcgg gtgttcgctg cgtttaaact tggcgcgtcc tccggtcaag
      721 cgcaacaggt accgacaccc gcgtcgcacc agggccccgt catcggcccg accgacaccg
      781 atactgaaga aggcgtctag cgcaccatga cgtaccccac ttcgcgcgaa tggatattct
      841 cggtcaagac cttcgcggct tccatgctgg cgctgtatat cggcctcaaa ctggagttgc
      901 cgcgtcctta ctgggcgatg gcgacggttt atatcgtgtc gaatccgttc gtgggggcga
      961 cgcgttccaa ggcgctgtat cgcgcgctcg gcaccatgat cggcgcctcg gccgccgtgc
     1021 tgttcgtgcc gccgttcgtc gagtcgccct acttgttcag cgccgtggtc gcgttatgga
     1081 ccggcacact cctgcttctc gccatctccg agcgcacggc gcgcagctat gtgttcatgc
     1141 tggcggccta caccatgccg ttgatcgcgc tgccctccgt gatggacccg gcgggcgtct
     1201 tcgatctcgc ggtatcgcgc accgaggaga tcgtgctcgg cattgtgtgc gccagcgtgg
     1261 tcggcggcgc cattttgccg agccgcctcg cgcctacgct tatcgaacgg accgacgcgt
     1321 ggttccgcga cgccgctttc tacgcgaaag aaacgctctc agggcgaacc agcggcacgg
     1381 ctatctccgc gtgccggcag cggctcgcgg tcaccgtcaa cgcactcgaa ttcctgctga
     1441 gccagcttgc ctacgatcac acccggcccg atgtactggc acgcacgcag gcgctgcgtg
     1501 gccggatgca gttgctgatg ccgatcatgt cggcaatcag cgacccgctg gccgcgctgt
     1561 tcaacggcca gaacccgccg ccagagggtc ttgagccgct actcgccg
//
LOCUS       DATASH010000104         5946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2190493,
            whole genome shotgun sequence.
ACCESSION   DATASH010000104 DATASH010000000
VERSION     DATASH010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5946
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2190493"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            510..2105
                     /locus_tag="VHV99_03185"
     CDS             510..2105
                     /locus_tag="VHV99_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX3634487.1"
                     /translation="MKSSIQRNIGPVALLLTGLGSIIGSGWLFGAWKAAKIAGPAAIC
                     AWVIGAVVILAIALTYAELGAMFPESGGMVRYARYSHGALVGFISAWANWIAIVSVIP
                     IEAEASIQYMSTWPYPWAHALFVDGSLTTNGLLLSAALVIVYFLLNYWGVKLFARANS
                     AITVFKFLIPGATILGLMLTGFHRENFGEVSTFAPYGWSSVLTAVATSGIVFAFNGFQ
                     SPINLAGEARNPARSVPFAVIGSILLALVIYVLLQIAYIGAVSPGDVMKGWSHFNFAS
                     PFAELAIALNLNWLAILLYVDAFVSPSGTGTTYMATTTRMIYAMERNNTMPKMFGNVH
                     PFYGVPRQAMWFNLFVSFIFLFFFRGWSSLAAVISVATVISYLTGPISLMALRRAATD
                     LERPLHIPGMKIIAPFAFVCASLILYWAKWPLTGEIILLMVVALPVYFYFQAKSGFSG
                     WGRDLKAAWWLVAYLPVMAILSLIGSKEFGGHGILPYGWDMLVVIAFSLVFYYWGVHS
                     GYRSEYLEERQTHDEVLEGIGAH"
     gene            complement(2248..3033)
                     /locus_tag="VHV99_03190"
     CDS             complement(2248..3033)
                     /locus_tag="VHV99_03190"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HEX3634488.1"
                     /translation="MNRLPSLFLSHGAPTLPIDPLLPSAEFGTLSARLPRPEAVLMLS
                     AHWGTAQPVASTSTAPETIHDFYGFPRQLYEIQYPAPGAPDVARRAAVLLGEHGMLTA
                     AQPHGLDHGAWVPMLLMFPEANVPVAQLSIQPHMDAAHHFRVGRALRSLKDDGVMVIG
                     SGQITHNLRAADFSARPEDADPRVAEFTDWFETKLAAHDIAALLDYRRQAPHAVLMHP
                     TDEHLLPVFAALGAADDDYSLAIQSLGTYQRSLAMTNYVFGTA"
     gene            complement(3254..3853)
                     /locus_tag="VHV99_03195"
     CDS             complement(3254..3853)
                     /locus_tag="VHV99_03195"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEX3634489.1"
                     /translation="METRAAAPHRLIVAITGATGAIYGIRLLETLRRLGGVESHLLIS
                     SAGWLNIQHELQLSKEDVHPLADVVHSVRDVGASIASGSFATDGMVVAPCSMKTLASV
                     AHGFSDNLITRAADVTLKERRRLVLLVRETPFNLAHLRNMTAVTEMGGVIFPPLPAFY
                     NQPASIDEMVDHTTARVLDLFALGPAMSPAWQGLRGAQE"
     gene            complement(3915..4226)
                     /gene="grxD"
                     /locus_tag="VHV99_03200"
     CDS             complement(3915..4226)
                     /gene="grxD"
                     /locus_tag="VHV99_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEX3634490.1"
                     /translation="MDTQQRIKQIVDDNNVVLFMKGTAQFPMCGFSGRAIQILKACGV
                     GEIKTVNVLEDDEVRQGIKQFSNWPTIPQLYVKGEFVGGSDIMMEMYESGELQQLFAA
                     A"
     gene            complement(4342..5184)
                     /gene="prmC"
                     /locus_tag="VHV99_03205"
     CDS             complement(4342..5184)
                     /gene="prmC"
                     /locus_tag="VHV99_03205"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX3634491.1"
                     /translation="MAPVTPAALMRASPLPSLEARILLTHVLGWRHTQLITRGEDPLE
                     FSIVERYRALETRRVAGEPVAQLVGAREFFGLDFEVTPDVLIPRPETELLVETALAAM
                     ESISRPRVLDLGTGTGAIAVAIASMRPDARVWALDRSAEALAVATRNAARLLDAKRPG
                     GALVLQQGDWYGSLDIALRFDVIVSNPPYIASGDPHLSEGDLRFEPRGALTDEADGLS
                     AIRKIVAGAPTRLAANGVLWIEHGYDQAEAVRSLLTAQGFTHVRSEQDLAGIERIGGG
                     QLPG"
     gene            complement(5191..>5946)
                     /gene="prfA"
                     /locus_tag="VHV99_03210"
     CDS             complement(5191..>5946)
                     /gene="prfA"
                     /locus_tag="VHV99_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEX3634492.1"
                     /translation="NIFLEIRAGTGGDESALFAGDLLRMYLRFAERNRWQVEMMSASE
                     SDLGGYKEVIVRIAGEAAYSKLKFESGGHRVQRVPATETQGRIHTSACTVAVMPEADE
                     IGEVEINPADLRIDTFRASGAGGQHINKTDSAVRVTHLPTGIVVECQDDRSQHKNKDR
                     ALKVLAARIKDKQSHEQQAKEAATRKSLIGSGDRSERIRTYNFPQGRLTDHRINLTLY
                     RLDAIMDGDLDELIAALVSEHQAELLASLGDAD"
BASE COUNT         1071 a         1773 c         1894 g         1208 t
ORIGIN      
        1 tcaaggaagg gggtaatggg accaaccgga gtaccgttga tgggcgaact acgaaagacc
       61 aattcactcg ccgcttgaaa tcctgcccga acgttatacg gcgatttcga tccaaggtca
      121 acactcttat cccattttta caaaaatttg ctgagtggga gtaaattttt cttacaaacc
      181 tttccatcgg ggctgaaatt ttgccaggga gaacccttgg tttcgctgca acgcgcaggt
      241 gcaaccgtta aactacccgc cgcacgtcgg ttgcacctca ttgggatggg ccgtcgtgct
      301 tgcatgcgcg tcacccgtct ccgcgccaca agcgacgtca cgcggtgccg gcatggttct
      361 gctgcaattt gcatgggacc ggggtaagta tgggaccaga gtaagtgctt tgcatgggac
      421 cggggtagcg cgctgaagcg cgaactctgg tcgacagcta aagcgccgct ctggtcgaca
      481 cactcactcc ggccgacaca ggagaggatg tgaaaagttc tatacaacgg aacatcggac
      541 cggtcgcgct actgctgacc ggcctgggtt cgatcattgg atcgggctgg ctgttcggtg
      601 cgtggaaggc tgcaaaaatc gccggacccg ctgctatttg cgcatgggta atcggcgcgg
      661 tcgtgattct cgcgattgca ttgacttacg cggaactcgg cgcgatgttc cccgaatcgg
      721 gcggcatggt gcgctacgct cgctattcgc atggcgcgtt ggtgggtttc atcagtgcat
      781 gggccaactg gattgctatt gtctccgtga ttcctatcga ggccgaagca tccattcaat
      841 atatgagtac ctggccgtat ccatgggcgc atgcgctatt cgtggatggc tcattaacga
      901 ccaatgggct attgctgtcc gccgcattgg tgatcgttta cttcctgttg aactattggg
      961 gcgtgaagct cttcgcgcgc gccaactcgg cgatcacggt cttcaagttc ctgattccgg
     1021 gcgcgaccat tctcggcttg atgctgacgg gtttccacag ggaaaacttc ggcgaagtga
     1081 gcaccttcgc gccgtatggc tggtcgtctg tgctgacggc cgtggcgacg agcggtatcg
     1141 tgttcgcctt caacggtttc cagagtccga tcaacctcgc cggcgaagca cgcaatccgg
     1201 ccaggagcgt gccgttcgcg gtgatcggct cgatcctgct ggccctcgtg atctacgtgc
     1261 tgctgcagat cgcgtatatc ggcgcggtga gccccggcga tgtgatgaag ggctggagcc
     1321 acttcaactt cgcttcgccg ttcgcggaac tggcgatcgc gctgaacctc aactggctgg
     1381 cgatcctgct gtacgtcgac gcgttcgtga gcccgagcgg caccggcacg acctacatgg
     1441 cgaccacaac ccgcatgatc tatgcaatgg agcgcaacaa cacgatgccg aagatgttcg
     1501 gcaacgtgca cccgttctac ggcgtaccgc gtcaggccat gtggttcaac ctgttcgtgt
     1561 cgttcatttt cctgttcttc ttccgcggct ggagctcgtt ggcggcggtg atctcggtcg
     1621 cgaccgtgat ctcgtacctg actggcccga tcagcctgat ggcgctgcgc cgcgcggcga
     1681 cggatctgga gcgtccactg catattccgg gcatgaagat cattgcgccg ttcgctttcg
     1741 tgtgcgcatc gctgattctg tactgggcaa agtggccgct gaccggcgaa atcattctgc
     1801 tgatggtcgt cgcgctgccg gtgtacttct acttccaggc aaagtcgggt ttcagcggct
     1861 gggggcgtga tctgaaggcg gcatggtggc tcgtggccta tctgccggtc atggcgatcc
     1921 tgtcgctgat tggcagcaag gaattcggcg gccatggcat cctgccgtac ggctgggaca
     1981 tgctggtggt gatcgcgttc tcgctggtgt tctattactg gggcgtgcat agcggctacc
     2041 gttcggaata cctcgaagag cgtcagacgc acgacgaagt gctcgaaggc ataggcgcac
     2101 actaagttgc cgagctaagt ctactgccgg attctcgtga tccggcgcag tatgaaaaaa
     2161 gcccgcctga gttgctcagg cgggcttttt cgcttgcggc gccttgatcg tggtctgacg
     2221 tttcgatggc gttgcgtcga cgttgaatca ggcggtgccg aacacgtaat tcgtcatggc
     2281 gagcgaccgt tgataggttc caagcgactg aatcgccagc gaataatcgt cgtccgccgc
     2341 acccagcgcg gcgaatacgg gcagcagatg ttcgtcggtc ggatgcatca gcaccgcgtg
     2401 cggtgcctga cggcgatagt cgagcaacgc ggcgatgtca tgcgcggcga gcttggtctc
     2461 gaaccagtcg gtgaattcgg ccacgcgcgg atcggcgtct tccgggcgcg cggagaagtc
     2521 cgcggcgcgc aggttgtggg tgatctgccc cgagccgatc accatcacgc cgtcgtcttt
     2581 cagcgagcgc agcgcgcgac ccacgcggaa atggtgggcg gcgtccatgt gcggctggat
     2641 cgacaattgt gcgaccggca cattggcttc cgggaacatc agcagcatcg gtacccacgc
     2701 gccgtggtcg agcccatgcg gctgcgcggc ggtgagcatg ccgtgttcgc cgagtagcac
     2761 agcggcacgg cgcgccacat cgggcgcgcc cggcgccgga tattgaatct catataactg
     2821 acgcgggaag ccgtagaaat cgtggatggt ttccggcgcc gtggacgtgc tcgccaccgg
     2881 ctgcgccgtg ccccaatggg ccgacagcat cagaacggct tctggacgcg gaagtcgcgc
     2941 cgacagcgtg ccgaattcgg cggatggcag gagcgggtcg atcggcagcg taggcgcgcc
     3001 gtgagaaagg aaaagcgacg gtaagcgatt catggcagta acgccgccgc aagaatgcgg
     3061 cgagaaagtg gggtggctat caatataggt ccgtacccgc gcttgataaa cgggtgaaaa
     3121 cggaattgat tatgtcgcag gcgttattaa tggtggtgga gcgattttcg gacgcggctt
     3181 gtctcgctcg ccgggtcaca gcaacgtagc gccgggcttc gcgccatgtg cggcgcggta
     3241 tgttggcccg gaattattcc tgcgcgccgc gcaatccttg ccaggccggc gacatagcag
     3301 gcccaagtgc gaacagatcc agcacgcgcg ccgtcgtgtg atcgaccatt tcgtcgatcg
     3361 aggcgggctg gttatagaag gcgggcagag gcgggaagat cacgccgccc atttcggtga
     3421 cggccgtcat gttacgcaga tgcgcgagat tgaacggcgt ttcgcgcacc agcagcacga
     3481 gccggcggcg ctccttgagc gtgacatcgg cggcgcgggt aatcaggttg tcggaaaaac
     3541 cgtgcgcgac gctggcaagc gtcttcatcg agcagggagc gacgaccatg ccgtcggtgg
     3601 caaacgagcc ggacgcgatg ctcgcgccga cgtcgcgcac cgaatggacg acatccgcga
     3661 gcggatgcac gtcttctttg ctcaactgga gttcatgctg gatattgagc cagccggcgc
     3721 tcgaaatcag cagatgactt tcgacgccgc ccagccggcg cagcgtctcg agcaaccgga
     3781 tgccgtagat ggcgccggtt gcgccggtga tcgcgacgat cagccggtgt ggtgccgcag
     3841 cgcgagtttc catggctcgc ggcgccttat cggctgacag cagaggttcg gacagcggcc
     3901 actacaacgg ccacttaagc cgcggcaaac agctgctgca gttcgcccga ttcgtacatt
     3961 tccatcatga tgtccgagcc gccgacgaat tcgcccttca cgtagagctg cggaatggtc
     4021 ggccagttcg agaactgctt gatgccctgg cggacttcgt cgtcttcgag cacgttgacg
     4081 gttttgattt caccgacgcc acacgccttc aggatctgga tggcgcggcc ggaaaagccg
     4141 cacatcggaa actgagcggt gcccttcatg aagaggacga cgttgttgtc gtcgacgatt
     4201 tgcttgatgc gttgttgcgt atccatgact gaccttgcgg ttccgtgatg tgtagggaat
     4261 aggctgaaat gatagcggat ttcggtacgg ctggccgagg gccgcttttt cctcctctgc
     4321 gatcgaggcc atccgccgag gtcagccggg caactgcccg ccgccgattc gctcgatgcc
     4381 ggcgagatcc tgctccgagc gcacgtgcgt aaaaccttgc gccgtcagca agctccggac
     4441 ggcctctgcc tgatcgtacc cgtgttcgat ccacaatacg ccgttggcgg ccaaacgtgt
     4501 gggcgcgccc gcgacgatct tacggatcgc gctgagcccg tcggcttcgt cggtgagcgc
     4561 gccgcgcggt tcgaagcgca gatcgccttc cgacagatgc ggatcgccgc tggcgatata
     4621 cggcgggttg ctgacgatca cgtcgaagcg caaggcgatg tcgagcgagc cgtaccagtc
     4681 gccttgctgc agtaccaagg cgccgccggg gcgtttggcg tcaagcaggc gcgccgcatt
     4741 gcgcgtggcg accgccagcg cttcggccga ccgatccagt gcccaaaccc gcgcatcggg
     4801 ccgcatcgac gcaatcgcta cggcaatcgc gccggtgccg gtgccgagat cgagcacgcg
     4861 cggccgcgaa atgctttcca tggctgccaa cgccgtttcg accagcaatt cggtttcggg
     4921 gcgcgggatc agtacgtcag gcgtcacttc gaaatcgagc ccaaaaaact cgcgcgcgcc
     4981 gaccagttgc gctaccggct caccggcgac acgccgcgtt tccagcgccc gatagcgctc
     5041 gacgatgctg aattcgagcg ggtcctcgcc gcgcgtgatc agttgcgtat gccgccaccc
     5101 aagcacgtga gtgagcagaa tccgcgcttc gagcgacggc agtggtgagg cgcgcatcag
     5161 cgcggcgggc gtgaccggag ccatccgcgc ttaatctgcg tcgccgagcg atgccagcaa
     5221 ctcggcctga tgctcgctga ccaatgccgc gatcaattcg tcgagatcgc cgtccatgat
     5281 cgcgtcgagg cgatacagtg tcagattgat ccggtgatcc gtcagacgcc cttgcgggaa
     5341 gttgtaggtg cgaatccgtt ccgagcggtc gcccgaaccg atcaggcttt tacgcgtggc
     5401 ggcttccttc gcctgctgct catgggactg cttgtccttg atacgcgcgg ccaaaacctt
     5461 cagcgcacga tccttgttct tgtgctgcga gcggtcgtcc tggcattcca cgacgatccc
     5521 cgtcggcaag tgcgtgacac gcacggccga gtcggttttg ttgatgtgct gaccgcccgc
     5581 gccggacgcg cggaacgtgt cgatccgcag atcggccgga ttgatctcga cttcgccgat
     5641 ctcgtccgct tccggcatta ccgcgaccgt gcaggccgac gtgtggatgc ggccctgcgt
     5701 ttcggtcgcc ggcacgcgct gcacgcgatg gccgcccgac tcgaacttga gcttggaata
     5761 ggccgcttcg ccggcaatcc gcacgatcac ttccttgtag ccgcccaagt ccgattcgct
     5821 cgccgacatc atctcgacct gccagcgatt gcgttcggca aagcgcagat acatgcgcag
     5881 caaatcgccc gcgaacagcg ccgattcgtc gccgcctgtt ccggcgcgga tttcgaggaa
     5941 gatgtt
//
LOCUS       DATASH010000105         4744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2190633,
            whole genome shotgun sequence.
ACCESSION   DATASH010000105 DATASH010000000
VERSION     DATASH010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4744
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2190633"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(260..1687)
                     /locus_tag="VHV99_03215"
     CDS             complement(260..1687)
                     /locus_tag="VHV99_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HEX3634493.1"
                     /translation="MNDFASPAYDVFVERHAARRAALACMITLAVVLGIGRFAFTPLL
                     PLMLHGSHFGQPQIDIQHGGWLASFNYAGYFAGAVACAALRVEPARMVRTGLVATVLL
                     TLAMGVTSQFWVWAVVRFVAGAVSAWTFVFASQWGLRRLAELGAHGWAGVIYTGPGVG
                     IVGTGLLVSAAGSYGATAGWIGFGLISAVLSIVVWPVFKGVTDAASGRAVLSAGGVNT
                     AVHARQRPAASQGAAASQGATTSQRPAANQGTATSQGPATSQRIATSQGPATSQTTAT
                     SQSHPSRRADAFWLILLYAVPGFGYIITATFLPVIARHALPGSFWPDLFWPMFGAALV
                     VGALLAARLPMHWDNRSLLAGCYVLQAAGIALGIVWPTAGGFSLGSILIGLPFTAITL
                     FAMREARRLRGDAAAGLMGYATAAYGIGQIVGPLVAAPVAEHTGSFSPALSLAAGALV
                     LGAIGLIIVARMPRAHRRMPDCGCN"
     gene            1806..2717
                     /locus_tag="VHV99_03220"
     CDS             1806..2717
                     /locus_tag="VHV99_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX3634494.1"
                     /translation="MDLAALTIFRAVVRENGVTRAAAKLNRVQSNVTTRIKQLEEQLG
                     TDLFIRDGRRLVLTPAGETLLPYAERLLALADEARHAVREDRPSGRLRLGAMESVAAT
                     RLPGLLARYHQQWPDVALELETGTTGKLIERVREFEIDAALVATPPDPAALGDLFETV
                     PVFREELVMLSPRGHRPIREVRDIALSTLIAFERGCVYRAYVEKWYLEHGVRPARVLE
                     LGSYHAIVACVAAGAGVAVAPRSVLDLQGDASNIAVHELHDIGEIETLLVWRRGHFSS
                     ALNALRQTLVSEEAAMDAPAVATTAAA"
     gene            complement(2781..>4744)
                     /gene="alaS"
                     /locus_tag="VHV99_03225"
     CDS             complement(2781..>4744)
                     /gene="alaS"
                     /locus_tag="VHV99_03225"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEX3634495.1"
                     /translation="TLTPLPKQCVDTGMGLERIAAVLQHVHSNYEIDLFQALIKAAGR
                     ETGVTDLTNNSLKVIADHIRACSFLIVDGVIPGNEGRGYVLRRIVRRAIRHGYKLGKK
                     GSFFHRMVPDLVAQMGGAYPELKDAEQRVTGVLRQEEERFFETIEHGMSILESALADL
                     DAKGGKTLDGELAFKLHDTYGFPLDLTADVCREREVTVDEAAFDEAMARQREQARAAG
                     KFKMAQGLEYSGAKTTFHGYEEIIFDDAKVIALYVDGASVKEVKHGQQAVVVLDHTPF
                     YAESGGQVGDQGVLANASVRFAVADTLKVQADVVGHHGTLEQGTLKVGDIVKAEIDAV
                     RRARTERNHSVTHLMHKALREVLGSHVQQKGSLVDADKTRFDFAHNAPMTDEQIRQVE
                     AIVNAEVLANAPGIVRVMPFDEAVKGGAMALFGEKYGDEVRVLDLGFSRELCGGTHVH
                     RTGDIGLFKIVMEGGVAAGIRRVEAITGDNAVRFVQDLDARINAAAAVLKAQPSELTQ
                     RIVQMQDQVKSLEKELSALKSKMASSQGDELAGQAIEVAGVHVLAATLEGADVKTLRE
                     TVDKLKDKLKSAAIVLASVEGGKVSLIAGVTADASKKVKAGELVNFVAQQVGGKGGGR
                     PDMAQAGGTEPANLPAALAGVKGWVEAQL"
BASE COUNT          832 a         1555 c         1499 g          858 t
ORIGIN      
        1 gttgtcggac gcgaaatgtt gcatggatcg gggcgcggcc ggcgacgggc gcgctgagat
       61 gacggaaagc ggtcatttta gtgcgccgaa ggattatttt tgtttttcgg tggttggggg
      121 cgccgggttg accaacgctg cgctggggaa atgccccccg atatgagcgc ctcgccgatc
      181 ctctgccgtt gaagacggcg ctgcgcacgc tgctgacaga cctgctcaag ccgctggaaa
      241 aatcactgat tgaaaaccgt cagttgcagc cgcaatcagg catgcggcga tgtgcacgcg
      301 gcatacgtgc cacgatgatc aggccgattg cgcccagtac caacgccccg gccgccagcg
      361 acaacgccgg cgaaaaggag ccggtatgtt cggcgaccgg cgcggccacc aacgggccga
      421 cgatctgccc gatgccgtac gccgcggtcg cataacccat caggccggcc gcagcgtcgc
      481 cacgcaggcg ccgggcttcg cgcatcgcga acagggtgat tgcagtgaat ggcagaccga
      541 tcaggatgct gccgagcgaa aagccgcctg ctgtcggcca gacaatgccg agcgcaatcc
      601 ctgctgcttg cagcacgtaa caaccggcga gcagcgagcg gttgtcccaa tgcatcggca
      661 ggcgtgccgc gagcaatgcg ccgacgacca acgccgcgcc gaacatcggc cagaacagat
      721 cgggccagaa cgagcccggc agcgcgtgcc gcgcgatcac cggcaggaac gtcgcggtga
      781 tgatgtagcc gaagccgggc acggcgtaga gcaggatcag ccagaaggcg tcggcgcggc
      841 gtgaggggtg gctttggctt gtggcggtgg tttggcttgt ggcggggcct tggcttgttg
      901 cgatgcgttg gcttgttgcg gggccttggc ttgttgcggt gccttggttc gcggcggggc
      961 gttggcttgt ggtggcgcct tggctcgcgg cggcgccttg gcttgccgcg gggcgttggc
     1021 gcgcatgaac ggcggtgttg acgccgcccg cagagagcac cgcgcgtcct gaggcggcat
     1081 cggtcacacc cttgaatacg ggccagacca cgatcgaaag caccgctgag ataagtccga
     1141 agccgatcca cccggctgtc gcgccgtagc tgcccgcagc gctgaccagc aagcccgtcc
     1201 cgacgatgcc cacgcccggc cccgtataga tcaccccggc ccaaccgtgc gcgccaagtt
     1261 cggcgagccg ccgcaagccc cactgcgagg cgaacacgaa ggtccacgcg ctgaccgcgc
     1321 cggcgacaaa tcgcacgaca gcccacaccc agaattgact ggttacaccc atcgccagcg
     1381 tcagcaagac cgtcgcgacg agcccggtcc gcaccatgcg cgccggctcg acccgcaggg
     1441 ccgcgcaagc gaccgcacca gcgaaatagc ccgcgtagtt gaacgacgcg agccagccgc
     1501 cgtgctggat atcgatctgc ggctgaccga agtggctgcc gtgaagcatc agcggcagga
     1561 gcggcgtgaa cgcaaaacgc ccaatgccga gcaccacggc caaggtaatc atgcaggcaa
     1621 gcgccgcgcg gcgggcggca tgacgttcga caaatacgtc gtacgccggg gaagcgaaat
     1681 cgttcatgag caggcagtca atggattcga gccggcggtc accggatgct tcaatccata
     1741 ttagcttgac ctttcaatca cgaaaaatga ataattagga taccaagcat cgctaggaga
     1801 gaatcatgga tctggccgcc ttgaccattt ttcgggctgt cgtgcgcgaa aatggcgtga
     1861 cgcgcgccgc tgccaagctc aatcgcgtgc aatcgaacgt caccacgcgg atcaagcagc
     1921 tcgaggagca actcggcacc gacctgttca tccgcgacgg ccggcgtctt gttttgacgc
     1981 cggctggcga gacgttgctg ccttacgccg aacgtctgct cgcgctcgcc gacgaggcgc
     2041 gccatgcggt gcgcgaggac cgtccgagtg ggcgcttgcg tttgggcgcg atggaaagcg
     2101 tcgcggcgac gcgtctgccc ggcttgctcg cgcgttatca ccagcagtgg ccggatgtcg
     2161 cactggaact cgaaaccggc acgaccggca agctgatcga gcgtgtccgc gaattcgaaa
     2221 tagatgcggc cctggtggcc acgccgccag atcccgccgc gctcggtgat ctgttcgaga
     2281 cggtgccggt tttccgcgaa gagctggtga tgctgtcgcc gcgtggccat cggccgattc
     2341 gcgaagtgcg cgatatcgcg ctatcgacgc tgatcgcgtt tgagcgcggg tgtgtttacc
     2401 gggcgtacgt cgagaagtgg tatctggaac atggcgtgag gccggctcgc gtgctggagc
     2461 tgggctctta ccatgcgatt gtggcgtgcg tagcggctgg cgccggggtg gcggtggcgc
     2521 ccaggtcagt gctggatttg cagggcgacg ccagcaatat cgccgttcac gagcttcacg
     2581 atattggaga aattgaaacg cttctggtct ggcggcgggg gcatttttcg tccgcgctga
     2641 atgcgctgcg gcaaacgctg gtgtcagaag aggctgcgat ggatgcaccc gccgttgcaa
     2701 cgacggcggc tgcttgacac tctggccgcg cgggacgcgc tcatgtcact ggcgccccgc
     2761 gtagcctgat caaacccgaa tcaaagctgg gcttcgaccc aacccttcac gccggccagc
     2821 gccgccggca gattcgccgg ctcggtgccg ccagcttgcg ccatatccgg ccgcccgcca
     2881 cccttaccac cgacttgctg cgccacgaag ttgaccagtt caccagcctt taccttcttg
     2941 ctggcatctg ccgtcacacc ggcaatcaga ctcaccttgc cgccttcgac cgaggccagc
     3001 acgatcgccg cgctcttcag cttgtccttc agcttgtcga ccgtttcgcg cagcgttttc
     3061 acgtccgcgc cctcgagcgt tgcagccagc acatgcacgc cggccacttc gattgcctga
     3121 ccagcgagtt catcgccctg gctcgacgcc atcttcgact tcaacgcgct cagttccttt
     3181 tccagcgact tcacctgatc ctgcatctgc acgatccgct gcgtcagttc cgacggctgt
     3241 gccttcaaca cggccgcagc ggcattgatc cgcgcgtcga gatcctgcac gaagcgcacg
     3301 gcgttgtcgc ccgtgattgc ttccacgcga cggatgccgg cagccacacc gccttccatc
     3361 acgatcttga agagaccgat gtcgcccgtg cgatgcacgt gcgtaccgcc gcacagctcg
     3421 cgcgagaaac cgaggtcgag cacgcgcact tcgtcaccat acttttcgcc gaacagcgcc
     3481 atcgcgccgc ccttcaccgc ttcgtcgaac ggcatcacgc gcacgatgcc cggcgcgttc
     3541 gccagcactt ccgcgttgac gatcgcttcg acctgacgga tctgctcgtc ggtcatcggc
     3601 gcgttgtgcg cgaagtcgaa acgggtcttg tccgcatcga ccagcgaacc cttctgctgc
     3661 acgtgcgaac cgagtacttc gcgcagcgcc ttgtgcatca ggtgagtcac cgagtgattg
     3721 cgctcggtgc gggcacgacg caccgcatcg atttccgcct tcacgatgtc gccgactttc
     3781 agcgtgccct gctcgagggt accgtgatgg ccgaccacgt ccgcctgcac cttcagcgta
     3841 tcggcgaccg caaagcgcac gctcgcgtta gccagcacgc cctggtcgcc gacctggccg
     3901 cctgactccg cgtagaacgg cgtgtgatcg agcacgacga cagcttgctg gccgtgcttc
     3961 acttccttca ccgaggcgcc gtcgacatac agcgcgatta ctttcgcgtc gtcgaagatg
     4021 atttcttcgt agccgtggaa cgtggtcttc gcgcccgagt attcgaggcc ttgtgccatc
     4081 ttgaacttgc ccgccgcgcg agcctgctcg cgctgacgcg ccatggcttc gtcgaaagcc
     4141 gcttcgtcga ccgtcacttc gcgctcgcgg cacacgtctg ccgtcagatc gagcgggaag
     4201 ccgtaggtgt cgtgcagctt gaatgcgagt tcgccgtcga gagtcttgcc acccttcgcg
     4261 tcaagatcgg ccagcgcgct ttccagaatc gacatgccgt gctcgatggt ctcgaagaag
     4321 cgctcttctt cctgacgcag cacgccggtc acgcgttgct ccgcgtcctt cagttccgga
     4381 taggcgccgc ccatttgcgc gacgaggtcc ggcaccatgc ggtggaagaa cgaacccttc
     4441 ttgcccagct tgtagccatg acggatcgcg cggcgcacga tacggcgcag cacgtagccg
     4501 cggccttcgt tgcccggaat cacgccgtcg acaatcagga acgagcacgc gcgaatgtgg
     4561 tcggcgatca ccttcagcga gttgttggtc agatcggtca cgccggtttc gcgcccagcg
     4621 gccttgatga gcgcctggaa caggtcgatc tcgtagttgc tgtgcacgtg ctgtagaacc
     4681 gcggcgatcc gctccaggcc catgccggtg tcgacacact gcttgggcag cggcgtcagg
     4741 gtgc
//
LOCUS       DATASH010000106         4204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3772735,
            whole genome shotgun sequence.
ACCESSION   DATASH010000106 DATASH010000000
VERSION     DATASH010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4204
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3772735"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VHV99_03230"
     CDS             <1..399
                     /locus_tag="VHV99_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX3634496.1"
                     /translation="VAVGLGPFAGVLALWVHTTGVLAKLFAEAVEAIDPRPAEGVRAT
                     GATGLDEIIYGVLPQVLPLWISYALYRFESNVRSAMVVGMVGAGGIGVVLYEAIRSFD
                     YASTAAVLIMVIVVVTVIDLASAWLRERVT"
     gene            complement(571..2094)
                     /locus_tag="VHV99_03235"
     CDS             complement(571..2094)
                     /locus_tag="VHV99_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014724093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX3634497.1"
                     /translation="MRASVLSDWLAQRLDRGNGQPIYRQLHRLLQQAILSRELPAGSK
                     VPSSRLLAQELGIGRNTVTQVYEQLVLEGYVSSATGRGTFVADSAPDEIVGAPDAEAP
                     AATLESLPSPPPLPLRGNRALSTRGARLIAGAGVSKRQWGAFMPGVPDVTKFPARVWS
                     RLHNKYWRRLRPDLLTYAPGGGLAALRHALADYLRTSRSVRCTPEQIIITTGIHQSVD
                     LAVRLLSDPGDVIWTEDPCYWGVRSVLHVSGLQSRPIAVDEEGINPSADDLAHPPKLM
                     LVTPSHQYPLGMVMSLARRRMLLEYARQNQCWIIEDDYDSEYRYGSRPLASLQGLDTS
                     GQVIYVGSFGKTLFPGLRIGYLVVPEALAESFATASAELYREGQLLQQAMLAEFIAEG
                     HFTSHIRKMRTLYGQRRQALLDAAARRYGDALPAVGGDAGLHLVMQLPEGSDDRTVAA
                     AALERNIVVRPLSGYYAQPSLAPSGLLIGYACVPDEEIAPAFNTLADAIDERLVGFM"
     gene            2267..3550
                     /locus_tag="VHV99_03240"
     CDS             2267..3550
                     /locus_tag="VHV99_03240"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007746027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HEX3634498.1"
                     /translation="MKNAELKSRKDAATPRGVGVMCDFYAERAENAELWDVEGRRFID
                     FAAGIAVCNTGHRHPKILAAIRDQLDHFTHTAYQIVPYASYVELAEKINQRAPGDYPK
                     KTAFFTTGAEAVENAIKIARAATGRPGVIAFTGGFHGRTMMGMALTGKVAPYKIGFGP
                     FPADVFHAPFPNPLHGVTTADSLKAIEFLFKADIDPKRVAAIIFEPVQGEGGFYPAPA
                     EFVRALRKLCNEHGILLIADEVQTGFARTGKLFAMHHYDVVPDLMTIAKSLAGGMPLS
                     GVVGRADVMDAAAPGGLGGTYAGNPLAVASAHAVLDIIDEEKLCERATVLGDRVKAKL
                     IALQSEVPQIVDVRGPGGMVAVEFCKAGGTEPDADFTRGVQALALERGLLLLVCGVYS
                     NVVRFLFPLTIQDSVFDEAMGILEAVLKECVAVAV"
BASE COUNT          732 a         1253 c         1326 g          893 t
ORIGIN      
        1 gtcgcggtgg gactcggtcc gttcgccggc gtgctggcgt tgtgggtgca caccaccggc
       61 gtgctcgcca agctcttcgc cgaagcggtc gaggcaatcg atccgcgtcc ggccgaaggc
      121 gtgcgcgcca ccggcgcgac cggtctcgac gaaatcattt acggcgtatt gccgcaagtg
      181 ctgccgttgt ggatctcgta cgcgctatat cgctttgaat cgaacgtgcg ctcggcgatg
      241 gtggtcggca tggtcggcgc gggcgggatc ggcgtggtgc tgtatgaggc gattcgctcg
      301 ttcgattacg catccaccgc tgcggtgctg atcatggtga tcgtggtcgt gacggtaatc
      361 gatctggcgt ccgcgtggct gcgcgaacgc gtgacgtgac agttcgcgat tcctgagtgc
      421 cgagggttgg cgagacgcga cttcgccaac cctcatccgg tcgcttcggt acggcgtgtc
      481 agcgtgtcag cgtgtcagcg tgtcagcgtg tcagcattga gtcgacgtct gtcagagcgg
      541 ttcccggctg ccacaaattt ccggctttcg tcacataaag cccacaagcc tctcatcaat
      601 cgcatcagcc aatgtattga acgccggcgc gatctcctcg tccggcacgc acgcatagcc
      661 gatcagcaag cccgacggcg ccagcgacgg ttgcgcgtaa taccccgaca aaggccgcac
      721 cacgatattg cgctccagcg ccgcggcagc caccgtgcgg tcgtcactgc cctcgggcaa
      781 ttgcatcacc agatgcaaac cggcatcgcc gcccaccgct ggcagcgcgt cgccatagcg
      841 gcgcgcggcg gcgtccagca aggcctgacg acgttgcccg tacagcgtgc gcatcttgcg
      901 gatatgcgac gtgaaatgcc cttccgcgat gaactccgcg agcatcgcct gctgcagcaa
      961 ttgcccttcc cgatacaact ccgcgctcgc ggtcgcaaag ctctcggcca gcgcttcggg
     1021 cacgaccagg tagccaatgc gcaagcccgg aaacagtgtc ttgccgaagc tgcccacgta
     1081 aatcacctgc cctgacgtat ccagaccttg cagcgatgcc agcggccggc tgccgtagcg
     1141 gtattcgctg tcgtagtcgt cctcgatgat ccagcattgg ttttgccgcg cgtactcgag
     1201 caacatgcgc cgccgcgcaa gactcatcac catgccgagc ggatattgat gcgacggcgt
     1261 gacaagcatc agcttcggcg gatgcgcaag atcgtcggcg gagggattga tgccttcttc
     1321 gtcgaccgcg atcggccgcg attgcaggcc ggacacgtgc agcacgctgc gtacgcccca
     1381 atagcaagga tcttcggtcc agatcacatc gcccggatcg gacaggaggc gcacagccag
     1441 gtccactgac tggtggattc cggtcgtgat gatgatctgc tcgggcgtac agcgcaccga
     1501 gcgcgaagtc cgcagatagt cagccaacgc gtgccgcaac gcggccaacc cgccgcccgg
     1561 cgcgtaggtc aacagatccg ggcgcagccg gcgccaatac ttgttgtgca ggcggctcca
     1621 cactcgcgcc ggaaacttcg tgacatccgg caccccgggc ataaacgcac cccattgccg
     1681 tttcgacacg cccgctcccg cgatgagccg cgcgccacgc gtcgacaacg cgcggttgcc
     1741 ccgcaacggc agtggcggcg gcgatggtaa tgactcgagc gtcgccgccg gcgcttcggc
     1801 gtcgggcgcg ccgacgatct cgtcgggcgc gctatcggcg acgaacgtgc cccggcccgt
     1861 tgccgaactc acatagcctt cgagcaccaa ttgttcgtag acctgcgtga cggtattgcg
     1921 cccgatgccc aattcctgcg ccagcagccg cgacgacggc accttgctgc ccgccggcaa
     1981 ttcgcgcgac aggatcgcct gttgcaacag ccgatgcagc tgacgataga tcggctggcc
     2041 gttgccacga tcgaggcgct gcgccagcca gtcggacaaa acgcttgcgc gcatgattgg
     2101 ctcctacatt tttattgaaa tggctctgaa attaagagcc aaatctcatt atagtcgctg
     2161 catgtgctgc gctaagcgcg cagcgtccgc aggcggcccc ccgcaggcgc atcctgaagg
     2221 cggccccgcc agcggcccgt aaaaccgagg agcgaaggag atgttcatga agaatgctga
     2281 actgaagagc cgcaaagacg ccgccacacc gcgcggcgtc ggggtgatgt gcgatttcta
     2341 cgccgagcgc gccgagaacg ccgagttgtg ggacgtcgaa ggccgccgtt tcatcgattt
     2401 cgctgcgggt attgccgtgt gcaacacggg ccaccgccac ccgaagatcc tggcggcgat
     2461 ccgcgaccag ctcgatcatt tcacgcacac cgcgtatcag atcgtgccct acgcgtcgta
     2521 cgtcgaactg gctgaaaaga tcaaccagcg cgcgccgggc gactacccga agaagaccgc
     2581 ctttttcacg accggcgcgg aagccgttga aaacgcgatc aagattgcgc gtgccgcgac
     2641 tggccgtccg ggcgtgattg cgttcacggg cggcttccat ggccgcacga tgatgggcat
     2701 ggcgctgact ggcaaggttg cgccgtacaa gattggcttt ggtccgttcc cggcggacgt
     2761 gttccatgct ccgttcccga atccgctgca cggtgtgacg acggccgatt cgctgaaggc
     2821 gatcgaattt ctgttcaagg ccgacatcga cccgaagcgt gtggcggcga ttattttcga
     2881 accggttcag ggcgaaggcg gcttctatcc ggcgccggcg gagttcgtgc gtgctctgcg
     2941 caagttgtgc aatgagcatg gcattttgct gattgccgac gaagtacaga cgggttttgc
     3001 ccgtaccggc aagttgtttg caatgcatca ctatgacgtg gtgcctgatc tgatgacgat
     3061 tgccaagagc cttgcaggcg gcatgccgtt atcgggtgtg gttggacgtg ctgatgtgat
     3121 ggatgcggcg gcgcctggtg gtttgggcgg cacgtatgcg ggcaatccgc tggcggttgc
     3181 gtcggcacat gctgtgctgg atatcatcga tgaagagaag ctttgcgagc gggctactgt
     3241 tttgggtgat cgggttaagg cgaagctgat tgcgctgcag agcgaagttc cacagatcgt
     3301 cgatgtgcgc gggccgggtg ggatggttgc tgttgagttc tgcaaagcag gtgggacgga
     3361 gccggatgct gatttcacca ggggggttca ggcgctggcg ctcgagcgcg ggttgttact
     3421 gctggtttgt ggtgtttatt cgaatgtggt gcggtttctg tttccgctta cgattcaaga
     3481 ttccgttttt gatgaggcga tggggatttt agaagctgtg cttaaggaat gtgttgctgt
     3541 ggctgtttga tggggttttg gcctgcggcg ctatttgtct gtgtgcctgc ggtgttggcc
     3601 tttccttggt ctggattttt ggcctttgct tgaactggct ctttggcctt tccttgaatt
     3661 ggtattggtc tattagcgtc gcccctgtgc ggggcgggca cctacttttc tttgcctgcc
     3721 gcaaagaaaa gtaggcaaaa gaaagcggct cacaccgcca gctcataagc ggggcccccg
     3781 cacagtcgcg gtagtggtgc atctggaatc cgtgttctcg cacattcggc gttggtgaca
     3841 aagggctcat acttcccgcc tcgcactgcg tgctcgtcgg aacggggcat caggaagatc
     3901 ggcggggtcg ctccggaatc gtgtgaatca tctgctcttc gcaaagccgt catgcttccg
     3961 cagcgctgcg cgtgtcggtg cggtgattcg taaagccctc tgggatgcac tggtgttggg
     4021 tgtgcacgtc gcttgatttg ctttggtttg cttgtgtcgt gcaattgtgc gcaactttgc
     4081 gttgctaacg cgagtcaatc gtgagaagaa tagctgtcct tcatcgttta cgtccccact
     4141 cttgcccgtc gccgatgacc tatcccattc tcaccacgct ctctcgtact cgtcttcgtt
     4201 ccgc
//
LOCUS       DATASH010000107         3099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3772862,
            whole genome shotgun sequence.
ACCESSION   DATASH010000107 DATASH010000000
VERSION     DATASH010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3772862"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VHV99_03245"
     CDS             <1..399
                     /locus_tag="VHV99_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006025343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX3634499.1"
                     /translation="VAVGLGPFAGVLALWVHTTGVLAKLFAEAVEAIDPRPAEGVRAT
                     GATNLDEIIYAVLPQVLPLWISYALYRFESNVRSAMVVGMVGAGGIGVVLYEAIRSFD
                     YASTAAVLIMVIVVVTAIDLASAWLRAKVI"
     gene            complement(503..1261)
                     /gene="phnF"
                     /locus_tag="VHV99_03250"
     CDS             complement(503..1261)
                     /gene="phnF"
                     /locus_tag="VHV99_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HEX3634500.1"
                     /translation="MTLNHTAAPGAQLERGAGVAVWRQIEQILAAEIVASGFGEDGRL
                     PSEGELARRFDVNRHTVRRAMLGLAAQGLVHVEQGRGTFVQPGAIDYTIGRRTRFTEN
                     LRQQHHAAGGAMLSAARVKAEPSVAKALGLRAGVQVYRIESLHESDGVPLTFARNWYP
                     VARFADLPEVLERTGGMTRALAEFGVADYLRKWSRIGSVLPEPEVARRLNINRQQPVL
                     RVENVDVDLQGTPIKYGVTHFAADRVQLMVEHDV"
     gene            1438..1638
                     /locus_tag="VHV99_03255"
     CDS             1438..1638
                     /locus_tag="VHV99_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634501.1"
                     /translation="MSASSGEAVEISEIDIMVRVEDGKIRDAERGPTSAREAEELGRR
                     LSAAQSDIGGPSAESRYPSCEP"
     gene            complement(1617..2642)
                     /locus_tag="VHV99_03260"
     CDS             complement(1617..2642)
                     /locus_tag="VHV99_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX3634502.1"
                     /translation="MEFRQLGLSGLKVSTLTLGTMTFGSPTDEASAARIVDKAHEQGV
                     NSIDTADVYSGGESERVVGRCIAAQRDRWVLATKIASPLVEGDVNARGASRKHMIGAV
                     EASLKRLNTDYIDLLYLHAEDHHTPVAETVRALNDLIRAGKIRYYGLSNHRGWKIAEF
                     AHTAQALGLDAPVASQPLYNIANRQAEAEQLTAAHRYGLGVISYSPLARGVLTGKYEL
                     GVTPASDTRAGRGDRRLQQAEWRPESLELARRVREHAQARGLSAGQFALAWVLNSRYI
                     SSTIAGPRTEAQWDDYQPALSYRLDAEDEAFVDSLVTTGHPSTPGFNDPKHPFFGRIP
                     RHGSHEG"
     gene            complement(2884..>3099)
                     /locus_tag="VHV99_03265"
     CDS             complement(2884..>3099)
                     /locus_tag="VHV99_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEX3634503.1"
                     /translation="VGSGAVVTKDVPPFAIVVGVPARVLRFRVDARTSERLQQIAWWD
                     WSPEQIDAALADFRALTAVEFAEKYGG"
BASE COUNT          566 a         1000 c          944 g          589 t
ORIGIN      
        1 gtcgcggtgg gactcggtcc gttcgccggc gtgctggcgt tgtgggtgca caccaccggc
       61 gtgctcgcca agctcttcgc cgaagcggtc gaggcaatcg atccgcgtcc ggccgaaggc
      121 gtgcgcgcca ccggcgcgac caaccttgac gagatcatct acgcggtgct gccgcaggtg
      181 ctgccgttgt ggatctcgta cgcgctgtac cgcttcgaat cgaacgtgcg ctcggcgatg
      241 gtggtgggca tggtcggcgc gggcgggatc ggcgtggtgc tgtatgaagc gattcgctcg
      301 ttcgattacg catccaccgc tgcggtgctg atcatggtga tcgtggtcgt cacggcaatc
      361 gatctggcct ccgcgtggct gcgcgcaaag gtcatctgat ggatgagcgc ggacaggcgg
      421 ccggcagccc ctcgccggcc cgtccgcgta gccaccgcgc cttctgaccc ggccggcttg
      481 ctcgcggtcc cggcggccat actcatacgt cgtgttccac catcagttgc acgcgatcgg
      541 cggcgaagtg cgtcacgccg tatttgatag gcgtgccctg cagatcgaca tcgacgttct
      601 cgacccgcaa caccggctgc tgccggttga tgttcagccg tcgcgccact tccggctcgg
      661 gcagcacgct gccgatgcgg ctccacttgc gcagatagtc ggccacaccg aactcggcca
      721 gcgcccgggt catgccgccg gtgcgctcca gcacctccgg caggtccgcg aaacgcgcaa
      781 ccggatacca gttgcgcgcg aaggtgagcg gcacgccatc ggattcatgc aacgattcga
      841 tccggtagac ctgtacaccg gcacgcaggc ccagcgcctt cgccacactc ggctcagctt
      901 tcacccgcgc cgccgacagc atcgcgccgc ccgccgcatg atgctgctga cgcagattct
      961 cggtgaaacg cgtacggcgg ccgatcgtat agtcgatcgc gccgggctgc acaaaggtgc
     1021 cgcgcccctg ctcgacgtgg acgagccctt gtgcggcaag ccccagcatc gcgcgtcgca
     1081 cggtatggcg gttgacgtcg aagcgcctgg ccagttcgcc ttcgctcggc aggcgcccgt
     1141 cttcgccgaa accgctggcc actatctcgg cagcaaggat ctgctcgatc tgcctccata
     1201 cggcaacccc cgcgccacgt tcgagctgcg cgcccggcgc ggcggtatgg ttcaatgtca
     1261 tggtgccgcc attcccttca gttcaaatat tcgttgtgac atgcattgta gtgcgccagc
     1321 cgtgatggaa atatgaaacc acggtcgttg caagcacaac cacattatct ctaaacgtct
     1381 atatgtttag atgaacagac gatcaaatga cggctcgctg catcaacagg aaccccgatg
     1441 agtgcctctt ccggtgaagc ggtcgaaatc agcgagatcg acatcatggt gcgcgtggaa
     1501 gacggcaaga tccgcgacgc ggaacgcggc ccgacttccg cgcgcgaagc cgaagaactg
     1561 ggacggcgac tatctgctgc ccagtctgat atcggcggac cgtccgcgga atcccgttat
     1621 ccctcgtgtg aaccgtgacg cgggatccgg ccaaagaaag gatgcttggg atcgttgaac
     1681 ccgggcgtcg aaggatggcc ggttgtgacg agcgagtcga cgaaggcttc gtcttccgcg
     1741 tccagacgat aactcaaggc cggttggtaa tcgtcccatt gcgcttcggt acgcggcccg
     1801 gcgatggtcg agctgatata tcggctgttc agcacccatg cgagcgcgaa ttgccccgcg
     1861 ctcaagcccc gtgcctgcgc atgctcgcga acgcgccgtg ccagttccag agactcgggc
     1921 cgccattcgg cctgctgcag gcggcggtca ccgcgccctg cgcgcgtatc ggatgcgggc
     1981 gtcacgccca gttcatactt tccggtcagc acgccgcgcg cgagagggct atacgaaata
     2041 acgccaagcc catagcggtg cgcggcagtg agttgttccg cctcggcctg ccggtttgcg
     2101 atgttgtaca gcggctggct ggccacggga gcatcgagcc ccagggcttg tgctgtatga
     2161 gcgaattcag cgatcttcca gccgcgatga ttcgacaaac cgtagtaccg tatcttgccg
     2221 gcgcggatca gatcattcag cgcacgcacc gtttcggcga ccggcgtgtg atgatcttcc
     2281 gcgtgaagat agagcagatc gatgtagtcg gtattcagac gcttcaggct ggcctcgacg
     2341 gccccgatca tatgtttgcg cgatgcaccg cgcgcgttga cgtcgccttc aaccagagga
     2401 ctggcgattt tggtcgccag cacccagcga tcgcgctggg cagcgatgca acgcccgacg
     2461 acacgctcag actcaccgcc ggagtaaacg tcggcggtgt cgatcgaatt gacgccctgt
     2521 tcatgtgcct tgtcgacgat gcgtgccgcg ctcgcctcat cggtcggact gccgaacgtc
     2581 atcgtaccga gcgtcagcgt cgaaaccttg aggccgctga ggccgagttg ccgaaattcc
     2641 atatgaacct tgctccacta atttaaaagg atctcagccc gtctggaaat tctcatgtga
     2701 catgtctatt tactatctgg acatctagat gaatttcatc gaggcaagag agttgctgtg
     2761 ttcacgctca ctacgccaga tcggaaagca atgcgagatt tccactcgcc aacgggcgaa
     2821 aatcgccgat cagacctttt ttgccacttg ccgcgcgaag ctaacagaca cgttaccgcc
     2881 gcgttaaccg ccgtacttct cggcgaactc gaccgcagtg agcgcgcgaa aatcggccag
     2941 ggccgcgtcg atttgctccg ggctccagtc ccaccaggcg atctgctgca gccgttccga
     3001 agttcgcgcg tcgacgcgaa agcgcagcac gcgcgccggc actccgacaa cgatagcgaa
     3061 cggcggcacg tctttggtca cgactgcgcc cgaaccgac
//
LOCUS       DATASH010000108         3085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3773100,
            whole genome shotgun sequence.
ACCESSION   DATASH010000108 DATASH010000000
VERSION     DATASH010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3773100"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="VHV99_03270"
     CDS             complement(<1..793)
                     /locus_tag="VHV99_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEX3634504.1"
                     /translation="MKLRVGYELSYECSQPTPMILMLNTHFSHAKDIVVADLLVTDPP
                     VPISQYRDLFGNLCSRIVAPVGRLGLRTTALLNVSSEMETRPGDGRGHPVEQLPDDTL
                     TFLLGSRYCETDLLVDTAWQTFGQCTPGRQTVLAICDFVHRHIGFDYGHARPTKTAWD
                     VYHERKGVCRDFAHLGVTLCRAMNIPARYCTGYISDVGLPPVTAPMDFAGWFEAYVGG
                     GWETFDPRNNAPRMGRVLMAYGRDAADVAISNAFGPAVLTHFAVHC"
     gene            complement(1763..2119)
                     /locus_tag="VHV99_03275"
     CDS             complement(1763..2119)
                     /locus_tag="VHV99_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007588956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HEX3634505.1"
                     /translation="MILYHTEPDSPLIEISVEGKITDHDLREAIERMRGDLELNGKTR
                     MLERIEHFTGIEPKALWTDMTLGVSVARKVTRAAVVADAGWIRASIHLARFFTKAEVK
                     AFHVNELEQARVWINT"
     gene            complement(2490..2696)
                     /locus_tag="VHV99_03280"
     CDS             complement(2490..2696)
                     /locus_tag="VHV99_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634506.1"
                     /translation="MAFVVVGGNIFGMIAPIATGYVIGATGSYDWASGIAGILLLVGA
                     TVVVTMARSPMTINRDASSAQVAV"
BASE COUNT          632 a          935 c          908 g          610 t
ORIGIN      
        1 cgcagtgcac cgcgaagtgc gtcagcacgg cgggcccgaa tgcattgctg attgcgacat
       61 cggcggcgtc gcgaccgtac gccattaaaa cgcgccccat gcgcggcgcg ttgtttcgcg
      121 gatcgaaggt ctcccagcca ccacccacat aggcttcgaa ccaccccgcg aaatccatcg
      181 gcgctgttac gggtggcaag cccacatcgc tgatatagcc ggtgcagtag cgcgccggta
      241 tattcatcgc gcggcatagc gtcacgccca gatgcgcgaa atcacggcag acgcctttgc
      301 gttcgtgata cacatcccac gcggttttgg tcggccgcgc atgcccgtag tcgaatccga
      361 tgtgtcggtg cacgaagtcg cagattgcca gcacagtctg gcgtcccggc gtgcactggc
      421 cgaaagtctg ccacgcggta tcgaccagca gatcggtttc gcaatagcgg ctgccgagca
      481 ggaaagtcag cgtatcgtcg ggtaactgct ccactggatg accccggccg tcaccgggcc
      541 gcgtttccat ttcacttgac acattgagca gcgctgtcgt gcgcaatccc agacgcccga
      601 caggcgcgac gatccggctg cacaggttgc cgaataaatc gcgatactgg ctgatcggca
      661 ctggcggatc tgtaaccagc agatccgcca cgacaatatc ctttgcatgg gaaaagtgcg
      721 tgttcagcat cagtatcatt ggcgtgggct gcgagcactc gtacgacagt tcgtatccga
      781 cgcgaagctt catggatcct ccgagattcg gcttattcgt ggagagcgct gggctgcatt
      841 cggagcgacg ccgcccgagg cagcgaaaag aatgatccta cgatgcaggg cttcacgctc
      901 caacaaaaag aactatctgc gtgtgccttg tctgcgacga ccaggaacga cactggcgca
      961 ccgacagcga aggtgcgttc tttcaaaacc agacaaccca gcgctcttcg tcacgcaatt
     1021 caaaaaacac aacctggttc ggacgaattg gttacacagg cgcgctttgc gcgtgggctt
     1081 ttcgtccgct ccgatctcac tgcttcaatc ttccggtagc cgttcgaacc cgacgcgccg
     1141 tgcggcgaca tgccgttctc acgctgctgc attaacgtcc cgctcgcggg ctgggtgctg
     1201 gcggcctctc cggcacgccc catgctctgt ccacccgccc cgatcgcgtt ttctgcgcgc
     1261 cttctgccgc ggccttcgca ctgctggggc tggtttgctc tgaagcacag gtgccagcgg
     1321 ccaggagtag agagcctatt accgccgcta tccgcgtcat catcatccgc agttcgggta
     1381 gcgctttggg cgcttctccg tgcataggag agcagcacgg gacatacccg ctccgacggc
     1441 gtcgcccacg aaacgacaat gagactgaac ccggtcatga agcgtcatcc gcgagcaact
     1501 tcgctcagac attcaggcgt cggctccact cgggaaccgg ccattcaatt cgcaatgttg
     1561 aagcgttgct tgtcggccag tccgatcatt cgagcctatt tggccaattt tgaatgaccg
     1621 gtctcagaaa aatcgaacag gcgctaacgc ccccttagcc gccagtgcaa gcaagagcgc
     1681 gaatgccaat ggcatcttga ttgcgcaggt caatcgcgct ccgacgtgca gccggactgc
     1741 tacaaaattc gccaggcggc gctcaggtgt tgatccagac gcgcgcctgc tccagttcgt
     1801 tgacgtggaa cgccttgact tcggccttcg taaagaaccg cgccagatgt atcgaggcac
     1861 ggatccagcc cgcatcggcc accacggcgg cgcgcgtgac cttgcgggcc acggaaacgc
     1921 cgagcgtcat atcggtccac agcgccttcg gctcgatgcc ggtgaaatgc tcgatgcgtt
     1981 cgagcatgcg ggtcttgccg ttcagttcga gatcaccacg catgcgctca atggcttcgc
     2041 gcagatcgtg gtcggtgatt ttgccttcga cggaaatttc gatgagaggg gaatcgggct
     2101 cggtgtgata taggatcatg gcaagaccgc ctgactagga agtcgttaca cgctcaggcg
     2161 cggcgcgggt gagcctggcg ttcgataatt ttttcacaat accccttttc acacggctcg
     2221 gttagccagg tttaacgccg tttagagtac gctgacggcc tcgctccgtt accatcttcc
     2281 cgcccgtgcg acacgtgtcg ctgcatggcc agccgttcgg ttggagtaag agcagggacg
     2341 tgcggcgcct cgcggccaac aagagacggc cgtccgtgta gttcgaacgg ccgcaacgtg
     2401 tgcgggaacg gacactggga cgaagcggac ggcagatagc atgcggcgtc gttcaaaccc
     2461 gtcgccactg acactgccgc gctatcacgt tacaccgcga cctgtgcact agaagcgtcg
     2521 cggttgatgg tcattggcga gcgcgccatg gtgacaacga ctgttgcgcc gacgagcaga
     2581 aggatgccag cgatgccaga cgcccaatca tagctgcctg tcgcgccgat cacatatccc
     2641 gttgcaatgg gcgcaatcat gccgaagata ttgccgccaa cgacaacgaa tgccatcgtc
     2701 ctgccgacat cgcgcgagtt cggaagaagg tcgtgcaaca gcgaaaagtt aagtgacgtc
     2761 gtggacgcaa ttccggtaag cgtgatcgaa aggccgaagg gcgggagcgc tacgattcga
     2821 cgtaaatggc acggcgagga tggccgagcc tgtgatcatt gcgactgcga ctgcgacagc
     2881 gttgcattgc gcaagattgc cgtaagttcg ggcctgaatt caattagcgt catcacggcc
     2941 gctgcgtagg ggcgatcatc ggatcgtcaa tatcgatgcc aagcaggcga agagccgtgt
     3001 cgatctgctt gatttgcggc ctgaagtccg tcttccactg ttcacgttcg gaatggacaa
     3061 tgagaccgtg gtacgtcgcc agcat
//
LOCUS       DATASH010000109         2307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4367366,
            whole genome shotgun sequence.
ACCESSION   DATASH010000109 DATASH010000000
VERSION     DATASH010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4367366"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..288
                     /locus_tag="VHV99_03285"
     CDS             115..288
                     /locus_tag="VHV99_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634507.1"
                     /translation="MEQEQVDRAEAPAETSLHLLQKYATFFKNPAWLVTERHLSGFDL
                     MRASSRAALTFIG"
     gene            376..2172
                     /locus_tag="VHV99_03290"
     CDS             376..2172
                     /locus_tag="VHV99_03290"
                     /inference="COORDINATES: protein motif:HMM:NF039846.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX3634508.1"
                     /translation="MATAKKKAPAQGDLLMGPVLGLRAAAADAPAVWRVTALIVIRSD
                     AEPPKLTVNGKADPTAPRTLRKTNDVQVLRYAIPVKQLAKDQVVAYSFGGTATWRFPV
                     PAVDQAPRFIYVSCNGFSSANAIKDLQHDAQNVWSDLITNHDGALLKAHERDWDEHQA
                     WVNSVLFDKGIPLRFHAMLMGGDQIYMDSIWDDIEALKNWSNLPVADQSGFAVDAKLA
                     DDIERYYFELYINRWRYARGKAPWPANAYDGTLDVACALATIPTLIMWDDHDIFDGWG
                     SYSPELQASPVFRALFAAARRNFWVYQLQMPDELLPPLALPDHPMRLPRFDPIDWNKV
                     AKKDPYAPPFLPQQPGFSHAGVFGSLAILVPDLRTERSQLQVIGGATWDAMTQWLTTL
                     PDTVEHLLFISSVPVAHPKIRLAEAIFSFGIARESVTKGLSDDVHDHWSNDDHEGQRK
                     RLVRNLIAFADGPRKRRVTMVSGDVHLAAWGVIEANDATTNNARRLNQLTSSAVVHPA
                     PSGVADFFFLSYMEHKAKAGEVIDSEHRLQMMELPGVGARLAPRRNWLAIELDEPTKR
                     LWATWRLEQDKGFSSHMLAVHPASSAAADGGG"
BASE COUNT          460 a          704 c          713 g          430 t
ORIGIN      
        1 aagaccgttc tgggtcgaat atcgtcccct gcggttggtg cagaatgagt ctcccttccc
       61 ttgaaaggag atcgtcatgg aagcgactat taccagcggc gtgccacgcg taccgtggaa
      121 caagaacaag ttgaccgggc agaagccccc gctgaaactt ccctgcactt gctgcaaaaa
      181 tatgccacct tcttcaagaa cccagcgtgg cttgtgacgg aaaggcattt gtcagggttc
      241 gacctgatgc gtgcatcgtc tcgcgctgca ctaaccttca tcggctaacc gcgccacgag
      301 gcgcgggtcc atcccgccgt ccgaccggcc caatcacgag cgcgacccgg cgacggcaac
      361 acgacgaggt gaagaatggc caccgcaaag aaaaaggcac ctgcgcaggg cgatctgctg
      421 atgggcccgg tgctcggcct tcgggccgcg gcggccgatg cgccggctgt gtggcgcgtc
      481 acggcgctga tcgtcattcg ctcggacgcc gagccgccga agctcaccgt caacggcaag
      541 gccgatccga ccgcgccgcg cacgctgcgg aaaacgaacg acgtgcaggt gctgcgctac
      601 gcgatcccgg tcaagcaatt ggctaaagat caggtcgtcg cgtattcgtt cggtggcacg
      661 gcaacgtggc gctttcctgt tcccgcagtc gaccaggcgc cgcgcttcat ttacgtgtcg
      721 tgcaacgggt tttcgtcggc gaacgcgatc aaggatctgc aacacgacgc gcagaatgtc
      781 tggtccgatc tgatcacgaa tcacgacggc gcgctgctca aggcccacga gcgcgactgg
      841 gacgagcatc aggcatgggt caattccgta ttgttcgaca agggcatccc gctgcgcttt
      901 cacgcgatgc tgatgggcgg cgaccagatc tacatggatt cgatctggga cgacatcgaa
      961 gcgctcaaaa actggagcaa tttgccggtc gcggaccagt cgggctttgc ggtggacgcg
     1021 aaactggccg acgatatcga gcgctactac ttcgagctgt acatcaaccg atggcgttat
     1081 gcgcgcggca aggcgccgtg gcctgcgaat gcgtatgacg gcacactcga tgtcgcgtgc
     1141 gcgctcgcga cgatccccac gctgatcatg tgggacgacc acgatatttt tgacggctgg
     1201 ggttcctaca gtccggaact gcaggcgagc cccgtgtttc gcgcactgtt cgcggcagcg
     1261 cgccgaaact tctgggtata ccagttgcag atgccggatg aactgctgcc gccgctggcg
     1321 ctccccgatc atccgatgcg acttccacgc ttcgatccga tcgactggaa caaggtggcg
     1381 aagaaagacc cgtacgctcc gccgtttttg ccgcagcagc cggggttttc gcatgcgggc
     1441 gtgttcggat cgctcgccat actcgtgccg gatctgcgca ctgaacgctc gcaactgcag
     1501 gtgattggtg gcgcgacgtg ggacgcgatg acgcaatggc tcacgacgct acccgatacg
     1561 gtcgagcatc tgctgttcat cagcagcgtg ccggttgcgc atccgaagat ccgcctcgcc
     1621 gaagcgatct tcagcttcgg tattgcgcgc gaaagtgtca caaaaggttt gtccgacgac
     1681 gtccacgatc actggtccaa cgacgatcac gaaggccagc gcaaacgtct cgttcgaaac
     1741 ttaatcgcgt ttgcggacgg cccacgcaag cgtcgcgtga cgatggtgtc aggcgacgtg
     1801 cacttggccg cgtggggcgt gatcgaagcg aacgatgcga ccacgaacaa cgcgcggaga
     1861 ctcaaccagc tcacgagttc ggcggtcgtt catccggcgc cttcgggcgt cgcggatttc
     1921 ttctttctgt cctacatgga gcacaaagcg aaagccggcg aggtgatcga ctccgagcac
     1981 cggctgcaga tgatggaact gcccggcgtg ggcgcgcgtc tcgcgccgcg ccggaactgg
     2041 ctcgcgatcg aactcgacga accgacgaaa cgtctgtggg cgacctggcg gctcgagcag
     2101 gacaaagggt tttcgagcca catgctggcg gtccatcccg cgagctccgc tgcggccgac
     2161 ggcggtggat gaaattgcgc tctcttgggt caacggcgcc tgactatatg ggtcgccgga
     2221 tgacggcact cggccgatat tgttgaaaaa gtcgctacat ggcttttgca atcggcaagc
     2281 aaaaaaatcg acctctcaga tcggccc
//
LOCUS       DATASH010000110         4203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1993414,
            whole genome shotgun sequence.
ACCESSION   DATASH010000110 DATASH010000000
VERSION     DATASH010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1993414"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="VHV99_03295"
     CDS             <1..610
                     /locus_tag="VHV99_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634509.1"
                     /translation="ACKIWPADPSLTLAVQALPKAGNTDDGGQYDVEVLVADGKSGAI
                     IAHEYEPAAISSDAIRFSQIDLDTARYQLTPQLRAFGVRVTYEGSSRVNPYEGQSLSL
                     YVLDGHALRRVVDDLAVQNNSGEGDGDCAGTFSAVTRTLAVGPANGAGYATLRVGETA
                     KESVSKQTSKDCVSTDRPAKRTTTTLEYDGTAYRVPKALHYE"
     gene            complement(706..2244)
                     /locus_tag="VHV99_03300"
     CDS             complement(706..2244)
                     /locus_tag="VHV99_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEX3634510.1"
                     /translation="MPQIGYQWLSNRYGVVPVHPFAVQSEIGRTRSTSTDGDIRREVY
                     PEPYRPTASLTDNLTFAFRHEGIHLEFLARLYARQSVREELEAWIAREPTGAYARRAC
                     FFYEWLMPEPLDAPGVSRGNYVDALNPEDFVVGTPVNNARWRVRDNLPGNRDFCPLIR
                     RVGAVQRAEAYNLAAPLAELEAAYGIDLILRSAVWLTVKESRASFLIEHEQDKEDRIR
                     RFAAVMESECGQHANPFDAETLDVLQRGILGQSALRYGIRRSPVYVGHIARYQPVVDY
                     IAPHWQHIEAMLAGLSRFLERTNGGASIVRAAAASFGFVYVHPMADGNGRISRFLIND
                     ILRRDGAVPAPIILPVSATITHSTRDRAAYDKVLERFSRPLMKHYADQYTFGKTEIAE
                     DGVEYNLHFRAYDDALPAWRYPDLTAHVIYLADVIDVTLTHEMRAEARFLHANDTARR
                     AIKEFLEAPDNDLDSIIRSVRQNGNSVSNGLRKRYPLLDERPGLSAQIMQAVTEAFAD
                     EQSD"
     gene            complement(2440..3309)
                     /locus_tag="VHV99_03305"
     CDS             complement(2440..3309)
                     /locus_tag="VHV99_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017773987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX3634511.1"
                     /translation="MQSLDSTGELLGALVAAARPLSLRDLAAAAGMPPAKAFPHLVSL
                     LKIGLLNRDAAGCFEAGPLALELGLIGLQRLSPTREAEPEVLELATSTGMSVAMAVLG
                     PLGPTVVRLEESARPLHVSLRVGTVMSLVNTAIGRVFAAYVADDVRSGLLAQDHLRLA
                     GAAEREIFAGAVDGPTTVTRKNAAPSQPQPQPQLTKAYAQRLAQIRADGIDTALSRPV
                     PGINTLAAPVLDHTGSICLVLALMGPSGSFDSELAGGPAQTLRAATLRLSRRFGWMAA
                     ASEPGKAASTNGK"
     gene            complement(3393..>4203)
                     /locus_tag="VHV99_03310"
     CDS             complement(3393..>4203)
                     /locus_tag="VHV99_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX3634512.1"
                     /translation="ETADGGKTQRGIQSVEVGGRVLLALAQARTPLALSDLATAAQIA
                     PGQAHAYLVSLSRLGLIKRDELSGRYEPGPLSLRLGLMHLENQPAFRAAVPRVAALAE
                     AIGFSVAICIAGPQGPTIVRYEHAGFPLHVNLHVGTVMALPATSTGRVFCAYLPREAL
                     EGMWTNQSGAASCAMTPPDEHAAFEAALHAIRARGLECSVDAPSPGISSLSAPVLDAD
                     GRLRLALTVIGSTGAIDVAADGPTACALLTAAHDISAELAAAPSLFASSAS"
BASE COUNT          802 a         1336 c         1333 g          732 t
ORIGIN      
        1 cgcgtgcaaa atctggcccg ccgacccttc gctgacgctg gccgtgcagg ccctgccgaa
       61 agccggcaat acagacgacg gcggacagta cgacgtcgaa gtgctggtcg ccgacggcaa
      121 gtcgggtgca atcatcgcgc atgaatatga accggcggct atcagttccg acgcgatccg
      181 gttcagtcaa atcgatctgg ataccgctcg ctatcaactc accccgcaac tgcgcgcatt
      241 cggcgttcgc gtgacctacg agggttcgtc gcgcgtcaat ccgtacgaag gtcagtcgct
      301 gagcttgtac gtgctggacg gtcatgcgct gcgccgcgtg gtcgacgatc tggcggtcca
      361 gaacaattcc ggcgaagggg acggcgattg cgccggcacg ttctcggcgg tcacgcgcac
      421 gcttgccgtt ggtccggcta atggcgccgg ctacgcgacg ctgcgggtcg gcgaaaccgc
      481 gaaggaaagc gtcagcaagc agaccagcaa agactgcgtc tcgaccgatc gtccggcaaa
      541 gcgcaccacc acgacgctcg aatacgacgg caccgcttac cgcgtgccga aagcattgca
      601 ctatgagtga cggcggcatg gcgcgctgct gcgccgcgtg ctatcggaaa gcgaaggcgc
      661 ggcggactga gaacaataac gcgtgacgtg ggcgccggcg gcggactagt cgctttgctc
      721 gtccgcaaac gcctcggtca cagcctgcat gatttgcgca ctcaaccccg gccgctcgtc
      781 gagaagtgga tagcgcttgc gcaagccgtt cgacacgctg ttgccgttct gccggacgct
      841 gcgaatgatg ctgtccagat cgttgtccgg cgcttcgaga aattccttga tggcgcgccg
      901 tgctgtgtcg ttggcgtgca gaaagcgcgc ctcggcgcgc atttcatgcg tgagcgtcac
      961 gtcgatcacg tcggcaagat agatgacgtg tgcggtcaga tcaggatagc gccaggcggg
     1021 cagcgcgtcg tcataggcgc gaaagtgcag gttgtattcg acaccgtctt ctgcgatctc
     1081 agtcttgccg aacgtgtact ggtcggcata gtgtttcatc agcgggcgcg aaaagcgttc
     1141 gagcaccttg tcgtaagcgg cgcgatcgcg cgtgctgtgg gtgatcgttg ctgagaccgg
     1201 caagatgatc ggcgcgggca ccgccccgtc gcgacgcaga atgtcgttga tcaggaagcg
     1261 cgaaatgcgg ccgttgccgt cggccatggg gtgcacatag acgaaaccga acgacgccgc
     1321 tgcggcgcgg acgatcgacg caccaccgtt ggtacgttcc agaaaacggc taagccccgc
     1381 gagcatcgcc tcgatatgtt gccaatgcgg cgcgatgtag tcgaccaccg gttgatagcg
     1441 cgcgatgtgt cccacgtaca ccggcgagcg gcggatgccg taacgcaggg ccgactgccc
     1501 gagaatgcca cgttgcaaga cgtcgagtgt ttcagcgtcg aacggattgg catgctgtcc
     1561 gcattcgctt tccatgactg cggcaaagcg gcggattcga tcctctttgt cctgctcgtg
     1621 ttcgatcaga aagctcgcgc ggctctcttt gaccgtcagc cagacggcgc tgcgcaagat
     1681 caggtcgatg ccgtaggccg cttccagttc ggcgagcggc gcggcaaggt tgtacgcttc
     1741 ggcgcgctga accgctccga cacgtcggat caacgggcaa aaatcccgat tgcccggcag
     1801 attgtcgcgc acgcgccaac gcgcgttgtt gaccggcgtg cctacgacga agtcttcggg
     1861 attcaacgca tcgacatagt ttccccgcga cacgccgggt gcgtccaatg gctccggcat
     1921 cagccattca tagaaaaagc aggcgcggcg ggcataggca cccgtgggtt cgcgggcgat
     1981 ccatgcctcc agctcctcgc gcacggattg ccgcgcatag agccgcgcga ggaattccag
     2041 atgaatgccc tcgtgccgga aggcgaaagt caggttatcg gtcaggctgg cggttgggcg
     2101 ataaggttcg gggtaaacct cgcggcggat gtcaccgtcg gtggaggttg agcgagtccg
     2161 gccgatctcg ctttgaacgg cgaacgggtg gaccggtacg acgccgtacc tgttggatag
     2221 ccattgataa ccgatctgcg gcatgatttt cgatccgggc ggctgagacg cgcgggagga
     2281 cacgtgtttc gcgccgtttc gctcagtttc gattactcgg ctcgcaaaac gattacaaaa
     2341 cgcgtgtttt ccaaaaaaac gattatacgg cgtcggcgca tcgtttcgat tactcggctc
     2401 gcaaaacgat taaaaaatgc cggtttctag aaaaaacgat tacttgccgt ttgttgaggc
     2461 cgcttttcca ggctcgcttg ctgccgccat ccacccgaac cgccgcgaca atctcagggt
     2521 cgccgcgcgc aaagtctgcg ccgggccgcc cgcgagttcg ctgtcgaagc ttccgctcgg
     2581 tcccatcagt gcgagcacga gacagatgct gccggtatgg tccagcaccg gcgcggccaa
     2641 ggtattgatg cccggcaccg ggcggctcaa ggccgtgtcg ataccgtccg cgcggatttg
     2701 cgcgagccgt tgcgcgtagg cttttgttag ctgcggctgc ggctgcggct gcgatggcgc
     2761 cgcgtttttg cgagtcacag tcgttggacc gtccaccgcc cccgcaaaaa tctccctctc
     2821 cgcggccccg gccagccgca aatgatcctg cgccagcaga ccactgcgca catcgtccgc
     2881 gacataggcc gcgaacacac gaccaatcgc cgtattcacc agcgacatca ccgtgccgac
     2941 ccgcaagctc acatgcagcg gccgggccga ttcttccagg cgcacgacgg tcggaccgag
     3001 tggcccgagc accgccattg cgacgctcat gcccgtcgac gttgccagtt caagcacttc
     3061 cggctcagct tcgcgcgtcg gcgacaaacg ttgcaggccg atcaacccca gttcaagcgc
     3121 tagcggcccg gcttcgaagc agccggccgc gtcccgattg agcagaccga tcttgagcag
     3181 actcaccaga tgcggaaacg ccttggcggg cggcatgccg gcagcggccg caagatcgcg
     3241 caagctcaat gggcgcgcgg cggcaacgag cgcacccagt aactcgccgg tgctatcgag
     3301 cgactggatg ccgcgctgcg gtttcgcatc ggtggatgtg gcagcagggg cagaagcccc
     3361 ggaggccgtg tacgagctgg agagagaatc ggtcacgatg cggaagaagc gaacaaggaa
     3421 ggtgctgccg caagctcggc gctgatgtcg tgagccgctg taagcagcgc gcaagccgtc
     3481 ggaccatcag cggcgacgtc gatcgcgccc gttgagccga tcacggtcag cgcgaggcgc
     3541 aagcggccgt ccgcatcgag caccggcgcg ctcaaactgc tgatgcccgg gcttggcgca
     3601 tcgacgctgc attccagccc gcgtgcgcgg attgcatgta aagccgcttc gaaagcggcg
     3661 tgttcatcgg gtggcgtcat cgcgcaactc gcggcacccg actgattcgt ccacatcccc
     3721 tccagcgcct cgcgtggcag ataagcgcaa aacacgcgac ccgtcgacgt ggccggcagc
     3781 gccatcaccg tgcccacatg cagattcaca tgcagcggaa agcccgcatg ctcatagcga
     3841 acgatggtcg gcccctgcgg ccccgcaata cagatcgcga cgctgaagcc gatcgcctcg
     3901 gctaaagccg cgacgcgcgg cacggcggcg cgaaacgccg gctggttctc cagatgcatc
     3961 aatccaagcc gcaacgacag cggcccgggt tcatagcggc ccgacagctc gtcgcgtttg
     4021 atcagaccta agcggctcaa gctcaccaga taagcatggg cctgccccgg tgcaatctgc
     4081 gcggcggtgg cgagatcgga caaagccagc ggcgtgcgtg cctgcgcgag cgccagcaac
     4141 acgcggccgc ccacttcaac gctctgaatc ccgcgctgcg tcttgccgcc atccgctgtc
     4201 tcg
//
LOCUS       DATASH010000111         2700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1993454,
            whole genome shotgun sequence.
ACCESSION   DATASH010000111 DATASH010000000
VERSION     DATASH010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1993454"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="VHV99_03315"
     CDS             <1..625
                     /locus_tag="VHV99_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEX3634513.1"
                     /translation="AHLRAIVEATDLPVNADFENGFGEDPADVAESVKLAVATGVAGL
                     SIEDSTGDDRGSLFPIEVAVERLRAARRAIDESGGDTLLIGRAENFFAGLPDLDDTIA
                     RLKAYAAAGADCLYAPGISTREQIEAVVAAVAPKPVNLLIGSTSTLTLQDVAALGVRR
                     ISVGGALARAAWGGFMQAAQALADGRFDGFGGAASGAQLNDIFKDRA"
     gene            779..1174
                     /locus_tag="VHV99_03320"
     CDS             779..1174
                     /locus_tag="VHV99_03320"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016347767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX3634514.1"
                     /translation="MAREIIRVEPLSTWLENWKAPTSAVTRHDGTVYVSGFPPFDPAT
                     GEVIDAPIERQTELVLEQMKLCLETAGSSLAHVLKCNVYCTSVEKFAAVNAVYQRYFP
                     VDPPARIFVNVPAWPGRFDIEIDCIAAVA"
     gene            complement(1236..1901)
                     /locus_tag="VHV99_03325"
     CDS             complement(1236..1901)
                     /locus_tag="VHV99_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX3634515.1"
                     /translation="MTNKLTLTDRLRESIEEAIATGDLPPGARLDEAELIARFEVSRT
                     PVREALLQLAAAGMVEMRPRHGAVVAKISLHRLIEMFEVMAELEAMCSRLAARRMNAQ
                     ELEALREAHAACQKARDAGSPDEYYHLNERFHCLIYHGSHNGFLHDQAMALHKLLRPY
                     RRLQLRVKGRVAASFDEHQAVVDALAAGDGEAAAAALRGHILVQGERFGDLIAALAQV
                     SAE"
     gene            2156..>2700
                     /locus_tag="VHV99_03330"
     CDS             2156..>2700
                     /locus_tag="VHV99_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011494379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEX3634516.1"
                     /translation="MKMQFVAVSVGVGVGIALIGGCGHGDDVNGPPPPVSHGAGFIKS
                     FQVISSVPAFAGATPTGAAGPYQVITAVVHGELDPDNPLNAGIVNIKNAPVGSDGYVA
                     YTTDVVILRPQNASAAKRVLFYDVVNRGSKIGAASFVGGGALDTGAAPSSSFPSLLRN
                     GYTILWSGWQGDVPLAGNSTTA"
BASE COUNT          472 a          865 c          850 g          513 t
ORIGIN      
        1 cgcgcatctg cgcgctatcg tcgaggcgac cgatctgccc gtcaacgcgg acttcgagaa
       61 tggcttcggc gaggatccgg ccgacgtggc tgaaagcgtc aagctggctg tcgcaaccgg
      121 cgttgccggt ctgtcgatcg aagattccac cggcgacgac cgcggttcgc ttttcccgat
      181 cgaggtcgcc gtcgaacggc tcagggccgc acgccgcgcc atcgacgaga gcggcggcga
      241 tacgctgctg atcggccgcg cggagaactt cttcgccggc ttgcccgacc ttgacgacac
      301 catcgcgcgt ctgaaggctt atgccgcggc cggcgccgac tgcctgtatg cgccgggcat
      361 ttcgacgcgt gagcagatcg aggccgtggt cgccgcggtg gcgcccaagc cggtcaatct
      421 gctgatcggt tcgacttcga cgctcacgct gcaagacgtg gccgcgctcg gcgtgcggcg
      481 aatcagtgtg ggcggcgcac tggcccgggc agcgtggggt gggttcatgc aggctgcaca
      541 ggcgctggcc gatggacgct tcgacggctt tggcggggca gcctcaggcg cgcaactcaa
      601 tgacattttc aaggaccgtg catgaggccc agcgtggtgt cccacgggac acctgacatg
      661 tggcctcaca tgtggcctga catgacaccc gacatgacac ccagcatgtg gcccgacatg
      721 acacccagca tgaggcccgg gcatcaggcc gcgcatgccc cttcacatgg agatgaccat
      781 ggctcgtgaa atcattcgtg tcgaaccgct gtcgacctgg ctggagaact ggaaggcgcc
      841 cacctcagcg gtgacccgcc atgacggcac cgtctacgtg tcgggctttc cgccgttcga
      901 tcccgcgacc ggcgaagtca tcgacgcgcc gatcgaacgg cagaccgaac tcgtactgga
      961 gcagatgaag ctatgcctgg aaacggccgg ttcatcgttg gcgcatgtgc tcaagtgcaa
     1021 cgtctactgc acgtcggtgg agaaattcgc tgccgtcaac gcggtctatc aacgctattt
     1081 ccccgtcgat ccaccggcgc gaattttcgt gaacgtgccc gcgtggcccg gtcgtttcga
     1141 catcgagatc gactgcattg cggccgtcgc atagcgcgtc gtcgaatagc ggtttgtcag
     1201 tttcacgccc aacgcacgtc ggcgctaact cgtcgctact cggcgctgac ttgcgcaagc
     1261 gccgcgatca gatcgccgaa gcgttcgccc tgcaccagaa tgtggcccct cagcgcggcg
     1321 gcggccgctt cgccgtcgcc tgccgccagc gcatccacga cggcctgatg ttcgtcgaat
     1381 gaggcggcga cgcgtccctt gacgcgtaac tgcaggcgcc ggtacgggcg cagcagctta
     1441 tgcagcgcca tcgcctggtc gtgtagaaag ccgttgtggc tgccgtgata gatcaggcag
     1501 tgaaagcgtt cgttcagatg gtagtactcg tccggactgc cggcgtcgcg cgctttctga
     1561 caggcggcgt gagcctcacg cagcgcctcc agttcctgcg cattcatgcg cctcgcggca
     1621 agccggctgc acatcgcctc gagctcggcc atgacctcga acatctcgat cagcctgtgc
     1681 aggctgattt tcgcgactac cgcgccgtgc cgcggccgca tctccaccat gccggctgcc
     1741 gccagctgga gcagcgcttc ccgcacgggc gttctggaca cctcgaagcg cgcgatcagt
     1801 tccgcttcat cgagccgcgc gccaggcgga agatcgccgg tcgcaatggc ttcctcgatg
     1861 gattcccgca gccggtctgt aagcgtgagc ttgtttgtca tgtttggact cccgacggaa
     1921 tcgagttgac cacaacgagc ctgccgacac cgtacgcgta aacgatagtt gagaacggtg
     1981 tccgccgaat tataaatacg tcaggcggtt ataaatatat aaattaaggc ttacaaaata
     2041 ttgacagggc ttccggacat atccgcggtc ttcgaccgat gacagtccgt caattccata
     2101 cgcgcccata cgcgcgtgca gtccttttcc ttgacccgtg ttcggagggc tgatcatgaa
     2161 gatgcagttc gtagctgtgt cggtgggagt cggtgtcggg atcgcgttga tcggcggctg
     2221 cggtcacgga gacgacgtca atgggccgcc gcctcctgtg tcgcacggcg ccggcttcat
     2281 caaaagcttc caggtcattt cgagcgtgcc ggctttcgcc ggcgcgacgc cgactggcgc
     2341 agcgggtccc tatcaggtga ttaccgccgt cgtgcacggc gagctcgatc cagacaatcc
     2401 gctcaatgcg ggcatcgtga acatcaagaa cgcgccggtg ggcagcgacg gttatgtcgc
     2461 ctatacgacc gacgtcgtga tcctgcgtcc gcagaacgca tcggccgcga aacgcgtgct
     2521 gttctacgac gtggtgaatc gcggcagcaa gatcggcgcg gcatctttcg taggcggcgg
     2581 cgcgctcgat accggcgcgg cgccatcgtc gagtttcccg tcgctgctgc gtaacggcta
     2641 tacgatcctg tggagcggct ggcaaggcga cgttccgctc gccggcaaca gcacgaccgc
//
LOCUS       DATASH010000112        11153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6542647,
            whole genome shotgun sequence.
ACCESSION   DATASH010000112 DATASH010000000
VERSION     DATASH010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 11153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11153
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6542647"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VHV99_03335"
     CDS             complement(<1..204)
                     /locus_tag="VHV99_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634517.1"
                     /translation="MPNPATTPVAAVAGSTDVTVNVQAGAELDVGGNNVILVRDGSTV
                     INRGTLHGVGDTFDAISAQGASGG"
     gene            complement(631..1989)
                     /locus_tag="VHV99_03340"
     CDS             complement(631..1989)
                     /locus_tag="VHV99_03340"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3634518.1"
                     /translation="MPTFLSSTKRCTALAADTASAHPTQPARAREIAQGRRWVIVAAL
                     FLFMLINFADKAVLGLVAIPLMHDMQLTHAQFGLVGSAFFVLFSISGIAVGLIADRVN
                     MKWLLAALALIWAVAQLPLAWPASFAVLLLCRVLLGAGEGPASPLALHVVYTWFDDRE
                     RNLPTAIVQQGATSGVILAGPLLTYISQRWHWHATFLTLGAVGIGWTILWLCLGKTGD
                     RQHNASPAFAPVAAAGPHAASLRLRRMLSDRTVIGVMLQCFVGYAVIAIGFTWVPAYF
                     RLGLGFAATQAGWLFALQVAAQIPLGIALAMASHRMLKRGVPSKVARGKLISAACVVS
                     GFAYCSLLFGAPPFIKVALMGLASALAIQTFTFGPMLVAEVAPAARRGALLAITNSIV
                     TTAGLIGPVAMGKLLGAAGDARGYEIGFAVTGVLLLIVGAVGFALIDPQRSKRRIEGI
                     AG"
     gene            2094..3050
                     /locus_tag="VHV99_03345"
     CDS             2094..3050
                     /locus_tag="VHV99_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3634519.1"
                     /translation="MHEVNSRRLTHLIALAEEGSFARAAERVHLSQPALSRSIQALEE
                     EVGMKLFDRAARGVAMTAAGRMLVERARRVLFETRCLFRDVELLKAHELGEVRIGLGP
                     YAAVVLLPDLLVQFARRFPKIKVSIELGEGDALLAKLRAEQIDFLVTDRRVPPVTPDV
                     TMQRLPRHEGGWFARPDHPLFARDPVQLAALREFPLVSVSLPAFMKDALHRLLKYRAH
                     EQIPLQIECNDVAVLKDVVAQTDAVLFATASAVRRDLEMRRLARIPLVNPPRLALEFA
                     LVYLAERTPSPAASSALGLAEQVMEDANRAARGLLHPDSAST"
     gene            3214..3906
                     /locus_tag="VHV99_03350"
     CDS             3214..3906
                     /locus_tag="VHV99_03350"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX3634520.1"
                     /translation="MTTTSPRSREAQQSSAVAAPRQTRSQQGLVRMLQAGRELLEASG
                     NLDDLSINDIVERAGTSIGAFYRRFDNKDSFFEVVQDAVMEEGLEHVRQTVERDPAWC
                     SNDAGTLADAVISFYVMTFRRNRGLYHASLLRASQRKASWDAVKSTNHEVLAMIVPRL
                     VSALAQSRGIKANDNATQSLAFEVRATLQMILGMLVNSVLNDPGPLSLSSRQLRPWLQ
                     IRVRRCLGLPDS"
     gene            4021..5637
                     /locus_tag="VHV99_03355"
     CDS             4021..5637
                     /locus_tag="VHV99_03355"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HEX3634521.1"
                     /translation="MSSCCPEQVRNPAFDAPHLGEPLDVAFDAGVNLAFAIAAPTVPP
                     RLTEHSRRMAQRIYRVAERVYCAVGYAIANIIFVVGDDGIVVIDATEAVSAAKRIYED
                     FCAIDPRHAELPVKAVVYTHNHTDHTGGVRAFVDEAALEARSVEIIAHRSLMDTVINN
                     ANLVAPILATRSAYSFGTLLPPGATGKVNAGIGPVLIAEPASFFAPTRVFDDQLDIVL
                     AGVRFEFRYAPSEADDEIVVWLPDLGVLLSAEVIQGECLANVHTLRGTRYRDPVRWYQ
                     TIDMMRGFNAAHMVPAHGRPVSGAQYVAEVLCVYRDAIQFIHDQTIRHMNFGLSPDEL
                     AEAIPALPPHLAEHAWLGEYYGTVKHSVRQVYSGQLGWFDGDPASLDPLPRAERSQRM
                     VGMMGGVEAVRAAARIALGDSDWRWAAELATLLVRFDTTDTDARNIKANALRELGYRT
                     LNTNWRNWYLSAARELEHAYDDLPFAGSAGLASTDVLRAQPLRNVFQRFSVNVDPERC
                     ADLQMTLALRVTDRDETHALELRRGVLQVH"
     gene            5641..5841
                     /locus_tag="VHV99_03360"
     CDS             5641..5841
                     /locus_tag="VHV99_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634522.1"
                     /translation="MPSGIDVQVAMETGTLYGLLRDLATRLPKCLESGTVVLERGTVE
                     QLHRFFDCFDRPARRMPALTTR"
     gene            5983..7635
                     /locus_tag="VHV99_03365"
     CDS             5983..7635
                     /locus_tag="VHV99_03365"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014722384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HEX3634523.1"
                     /translation="MEKIWLKSYPPGVPAEIDATQFNSVADLLEQSFQKHAAERAFVC
                     MGCELTYGELDAKSRHLAAWFQRLGLARGARVAVMLPNVLQYPVAMAAILRAGYVLVN
                     VNPLYTPRELGHQLNDSGAQAIVLLDTFSQTLEAVEAQTSVKHVVLTSIGEMLGVGSP
                     EIAADVPPTSRIALNDAIARGADAGFTPVALTRGDVAVLQYTGGTTGVSKGATLLHGN
                     LIANVLQSELWREPVYRGRTDIKQAITVAALPLYHIFGLTVSGLLTMRCGGMGILIPN
                     PRDLAGLIKALRGYAINSFPGVNTMYNALLNEPDFKTLDFSKLVQSNGGGMAVQQAVA
                     QRWQALTGVPIVEGYGLSETSPCATTNLPTSTAFTGTVGLPLPSTDISIRDDAGNEVP
                     LGERGEICIRGPQVMAGYWNRPDETARAMTPDGFFKSGDIGVMTEEGFVKIVDRKKDM
                     ILVSGFNVYPNEIEDVVAKHPGVFEVVAVGVPDAEAGEVVKLYVVKKDPALTEADILA
                     FCKEQLTGYKRPKIIEFRSELPKTNVGKILRRALRDELKHSG"
     gene            7737..8123
                     /gene="gloA"
                     /locus_tag="VHV99_03370"
     CDS             7737..8123
                     /gene="gloA"
                     /locus_tag="VHV99_03370"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012399820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEX3634524.1"
                     /translation="MRLLHTMLRVGDLDRSIAFYNDLLGMKLLRRQDYPEGKFTLAFV
                     GYEDERDGAVLELTHNWDTPSYDLGNGFGHLAVEVDDAYAACDKIKAQGGKVVREAGP
                     MKHGTTVIAFVEDPDGYKIEFIQKKA"
     gene            8150..8548
                     /locus_tag="VHV99_03375"
     CDS             8150..8548
                     /locus_tag="VHV99_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634525.1"
                     /translation="MNNSNPYFKELGDIHAEIQALFDGSADHGVLDRIMARFAPQFTL
                     VMTSGIALDHAGVREIFAKLNGARPGSQISIRDMEIVSEYPSGAVVKYREYQRDKAGN
                     ANVRRSTAVMDLDAHGKVTWRHLQETICTA"
     gene            complement(8698..9585)
                     /locus_tag="VHV99_03380"
     CDS             complement(8698..9585)
                     /locus_tag="VHV99_03380"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HEX3634526.1"
                     /translation="MQKSPTSQPAVALDNRQLDLPLFAEPGSTSSSPTPSAPSGSQSG
                     QQPAVPLAPDGTRLRSLTIGSRTLHYALKRSARRSIGFAIDSTGLMITAPRWVTLADI
                     ETAITEKQRWIFTKLIEWQTRVEQRALPKVDWKDGAEVPYLGQPVRVKLGSPQGMLAF
                     NAEEAALQVPLPLQADPQQIKDRVQGWLQGEAKRLFGERLAIYAEKLGVNYRAYALSS
                     AATRWGSCSSDGKIRLNWRLIHFPLSIIDYVVAHELAHLREMNHSPRFWQTVESIFPE
                     FREARQTLKSHPPELLPTL"
     gene            complement(9630..10388)
                     /locus_tag="VHV99_03385"
     CDS             complement(9630..10388)
                     /locus_tag="VHV99_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX3634527.1"
                     /translation="MRFIRSLLLLIYFVLFTVPYATACFIAFPFMRADNRYWMAAGWC
                     RATLHTVRWLNGIRYRIDGFENLPDGPAVLLSKHQSAWETLAFPALMPRPLCYVFKRE
                     LLYVPFFGWALGMLKMVHIDRKEGKYAFESVIRQGKARMAEGAWVIMFPEGTRTPTGK
                     QGKYKTGGARFAIATGAPVVPIAHNAGRVWPRNSFLKYAGIVTVSIGKPIETTGLTPD
                     EVNTRVEQWIEAEMRRIDPTAYRAADSSSAAAPI"
     gene            complement(10429..10983)
                     /gene="gmhB"
                     /locus_tag="VHV99_03390"
     CDS             complement(10429..10983)
                     /gene="gmhB"
                     /locus_tag="VHV99_03390"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010101763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HEX3634528.1"
                     /translation="MPTKKVVILDRDGVINVDSDAFIKSPDEWVALPGALEAIARLNQ
                     AGYRVAIATNQSGIGRGLFDMNALNAMHLKMHRMAAAVGGRIDAVFFCPHTAQDHCEC
                     RKPKPGMLKMITERFEVDPEHTPVVGDAMRDLEAGAALGFPTHLVLTGKGRKTLAAGG
                     LPAGTQVHDDLRAFALDFLSDDHE"
BASE COUNT         2125 a         3452 c         3593 g         1983 t
ORIGIN      
        1 accgccgctc gcgccttgcg ccgagatggc gtcgaaggtg tcgccgaccc cgtgtaaggt
       61 gccgcggttg atgacggtgc tgccgtcgcg cacgaggatg acgttgttgc cgccgacgtc
      121 gagttcggca cccgcttgca cgttcaccgt gacgtcggtg ctgccggcaa ccgcggcgac
      181 cggcgtagtg gccggattgg gcacgctgct atcgcaggcg acggtctggc cgctggctgg
      241 cgcagcgttg tcgcaagctg cgaatgcagg ctcgcatgga agaaggattg ctatcccgag
      301 tgcggacgaa agggcttcga aaagacgcga acgaacagac acatgcagcg agcatccacg
      361 acgcatacgg ttctccagta gaacagggct ggtcagttcc tgctgggttt gttatgtgcg
      421 ctgcgccgtc tcttggaatg ccgcgtatgg acgatgtgca agagaatgga ttgtctgatg
      481 aatttagtca acggcacgaa tgacgcggaa cgggagtgtt atgccgtatg actgcatatg
      541 aagaaactgt tcgagtaggg cgtaaaggac ccctgcaaaa accgctgcaa aaaccgctgc
      601 aaaaacccct gcaaaagcgg cccaaaagta tcaccccgca atgccctcga tgcgccgctt
      661 cgaccgttgt ggatcgatca acgcgaaacc caccgcgccg acgatcaaca gcaacacgcc
      721 ggtcaccgcg aagccgattt catagccacg cgcgtcgcct gccgcgccga gcagcttgcc
      781 catcgcaacg gggccgatca gtcccgcagt ggtgacgatc gagttggtga tcgcgagcag
      841 ggctccgcgc cgcgctgccg gcgcgacctc ggcgaccagc atcggaccga aggtgaaagt
      901 ctggatcgcc agcgcgctgg caagtcccat caacgcgacc ttgatgaacg gcggcgcgcc
      961 gaacaacaat gagcagtacg caaagccact caccacgcat gccgcactga tcagtttgcc
     1021 gcgcgccacc ttcgacggca cgccgcgctt gagcattcgg tgcgatgcca tcgccagtgc
     1081 gatgccaagc gggatctgcg ccgcgacttg cagtgcgaac aaccagccag cctgagttgc
     1141 ggcaaagccg aggccgagcc ggaaatacgc gggcacccag gtgaagccga tcgcgatcac
     1201 cgcatagccg acaaagcatt gcagcatcac gccgatcacc gtgcggtcgc tcagcatgcg
     1261 ccgcaagcgc aatgacgcag catgaggccc ggctgcggcc accggcgcaa acgcgggcga
     1321 tgcgttgtgc tgacgatcgc ctgtcttgcc caggcacagc cacaaaatgg tccagccgat
     1381 gccgactgca ccgagcgtga gaaacgtcgc gtgccaatgc caccgctgcg agatgtaggt
     1441 gagtaacggt cccgcgagga tgacacctga ggtcgcccct tgctgaacaa tggcggtggg
     1501 aagattgcgc tcgcggtcgt cgaaccaggt gtagaccaca tgcagtgcga gcggcgaggc
     1561 cggcccttcg cccgccccca acagcacgcg gcacagcagc agcacggcga agctggccgg
     1621 ccacgcgagc ggcagctgcg cgaccgccca gatcagcgcg agcgcggcga gcagccattt
     1681 catgttgacg cgatcggcga tcaggcccac ggcgatgccc gagatcgaga acagcacaaa
     1741 aaacgcgctg ccgacgaggc cgaactgagc gtgcgtcaat tgcatgtcat gcatcagcgg
     1801 gatcgcgacg agaccgagca cggccttgtc ggcgaaattg atcagcatga acaggaacag
     1861 cgcggcgacg attacccagc ggcggccttg cgcgatctcg cgcgcgcgag cgggttgggt
     1921 tggatgagcc gacgccgtgt cggcggcaag cgcggtgcag cgcttcgtgc tgctgaggaa
     1981 agtgggcaaa gcgggtctcc gtttctggcg cggggggcgc tgggttttcg cccatttgag
     2041 gtggtataac tgaattgttc aagtgcattc tcgttatcgg gagcttgcaa accatgcatg
     2101 aggtcaattc ccgccgtctt acccatttga tcgcgctcgc ggaggagggc agtttcgcgc
     2161 gcgcggcgga gcgggtccat ctgagccagc cggccttgag ccgcagcatt caggcgctcg
     2221 aagaggaagt cggcatgaaa ctgttcgacc gcgccgcgcg cggcgtcgcg atgacggcgg
     2281 ccgggcggat gctggtcgag cgcgcccgcc gcgtgctgtt cgaaacgcgc tgcctgtttc
     2341 gcgacgtcga actgctgaaa gcgcatgagc tcggcgaggt gcgcatcggc ctcgggccct
     2401 acgcggctgt cgtgctgctg ccggacctgc tggtgcagtt cgcgcggcgc tttccgaaga
     2461 tcaaggtgtc gatcgagctt ggcgaaggcg atgcgctgct ggccaagctg cgcgccgaac
     2521 agatcgactt tctggtcacc gatcgccgtg tgccgcccgt gacgcctgat gtcacgatgc
     2581 agcgtctgcc gcgtcacgag ggcggctggt tcgcgcgccc ggaccatccg ctgtttgcgc
     2641 gcgacccggt gcagctcgcg gcgttgcgcg agtttccgct ggtctcggtg agcttgccgg
     2701 cattcatgaa ggacgcgctg catcgtctgc tcaagtaccg cgcgcacgag cagattccgt
     2761 tgcagatcga atgcaacgac gtggcggtgc tgaaggatgt cgttgcgcag accgatgcgg
     2821 tgctgttcgc cacggcgtcc gccgtgcggc gcgatctgga aatgcggcgg ctcgcgcgca
     2881 ttccgctcgt caatccgccg cgtctcgcgt tggaattcgc gctggtgtac cttgccgagc
     2941 gcacgccgtc gcctgccgcg tcttccgcgc tgggactcgc cgagcaggtg atggaggacg
     3001 ccaaccgcgc tgcccggggc ttgctgcatc ccgactcagc gtcgacatga atgggtcgct
     3061 gaacgcccgc ctggatgatc gtatgatcgg cccccgtgat taccctggaa gcgattgaca
     3121 cagcgatccc aatcgcggac gatccggaat ccagattccg gtttgccgtg agtcttcatt
     3181 ttctcagagc agcgaaccga agcagaaacc tgcatgacga cgacttcacc acgcagccgc
     3241 gaggcccagc agtccagcgc cgtcgcggcg ccgcgccaaa cgcgcagtca gcaagggctc
     3301 gtgcgcatgc tgcaagccgg gcgcgagttg ctcgaagcga gcggcaatct cgacgatctg
     3361 tcgatcaacg acatcgtcga acgtgccggc acgtcgatcg gcgcgttcta ccggcgcttt
     3421 gataacaagg acagcttctt cgaagtcgtc caggacgcgg tgatggaaga gggcctcgaa
     3481 catgtgcgcc agaccgtcga gcgtgacccg gcctggtgct ctaacgatgc aggcacgctt
     3541 gccgacgcgg tcatttcgtt ttacgtgatg acgttccggc gcaatcgcgg tctatatcac
     3601 gcgtcgttgc tgcgagcttc gcagcgcaaa gcgagttggg acgccgtgaa atcgaccaat
     3661 cacgaggttc tggcgatgat cgtgccgcgc ctcgtcagtg cgttggcaca gtcacgcggc
     3721 atcaaggcca acgacaacgc tacccagtcg ctcgcattcg aagtgcgcgc cacgttgcaa
     3781 atgatcctcg gcatgctggt gaatagcgtg ttgaacgacc ccggtccgtt gtcgctctca
     3841 agccggcaac tgcggccgtg gctgcaaatt cgggtgcggc gctgcctcgg tttgccggac
     3901 agttgatttt ttttgcctaa aaaccggaat aagaattccg gttccattaa tagaaagcca
     3961 atcctcttaa gtgcagcgca atgtccgctg cgcgcagccg aatatccaag gagacaccgc
     4021 atgagctcat gttgccccga acaggtccgc aatcccgcct tcgacgcgcc gcatctcggc
     4081 gaaccgctcg atgtcgcgtt cgacgccggc gtgaatctcg ccttcgccat tgccgcgcca
     4141 accgtgccgc cgcgtctcac cgaacactcg cggcgcatgg cgcagcgcat ctatcgcgtc
     4201 gccgagcgcg tgtattgcgc ggtcggctac gcaatcgcaa acatcatctt cgtggtgggc
     4261 gacgacggta tcgtggtgat cgacgcgacc gaagcagtgt ccgccgcgaa acgcatctac
     4321 gaggacttct gcgcgatcga tccgcggcat gccgaactgc cggtcaaggc cgtggtctac
     4381 acgcacaatc acacggacca taccggcggc gtgcgcgcct ttgtcgatga agccgcgctc
     4441 gaggcgcgca gcgtcgagat cattgcgcac cgctcgttga tggacaccgt gatcaacaat
     4501 gcgaacctcg tcgcgccgat tctcgccacg cgttcggcct acagcttcgg cacgctgctg
     4561 ccgcccggcg cgaccggcaa ggtcaacgcg ggcatcggac cagtgctgat cgccgagccg
     4621 gcgagcttct tcgcgccgac ccgcgtgttc gacgaccagc tcgacatcgt gctggcgggt
     4681 gtgcgcttcg agttccgcta cgccccctcg gaagccgacg acgaaatcgt cgtgtggctg
     4741 ccggacctcg gcgtgctgct gtcggcggaa gtgatccagg gcgagtgcct cgcgaacgtg
     4801 catacgctgc gcggcacgcg ttaccgcgat ccggtgcgct ggtatcagac catcgacatg
     4861 atgcgcggct tcaacgcggc gcatatggtg ccggcgcatg gccggccggt atcgggcgcg
     4921 caatatgtcg ccgaggtgct gtgcgtgtat cgcgacgcca tccagttcat ccacgatcag
     4981 accatccgtc acatgaactt cggtctgagt ccggacgaac tcgccgaagc gattcccgcg
     5041 ctgccgccgc atctcgcgga gcatgcgtgg ctcggcgaat actatggcac ggtcaagcat
     5101 tcggtgcggc aggtgtatag cggtcagctc ggctggttcg atggcgatcc ggcgtcgctc
     5161 gatccgctgc cgcgcgccga acgttcgcaa cgcatggtcg ggatgatggg cggcgtggaa
     5221 gcggtgcgcg cggccgcgcg tatcgcgctg ggcgactcgg actggcgctg ggccgcggaa
     5281 ttggcgacgc tgctggtgcg cttcgataca accgacactg acgcgcgcaa tatcaaggcg
     5341 aacgcgctac gcgagttggg ctaccgcacg ctgaacacca actggcgcaa ctggtatctg
     5401 agcgcggcac gcgaactcga acacgcgtac gacgatttgc cgttcgcggg cagcgcgggt
     5461 ctcgcctcga cggacgtgtt gcgcgcccaa ccgttgcgca atgtgttcca gcgcttcagc
     5521 gtgaacgtcg atccggaacg gtgcgcggat ttgcagatga cgctggcatt gcgcgtgacg
     5581 gatcgcgatg aaacgcatgc gctggaacta cggcgtggcg tgttgcaggt tcactagcgc
     5641 atgccttcgg ggatcgacgt tcaggtcgcg atggaaaccg gcaccttgta cggcctgctg
     5701 cgcgacctgg cgacgcggtt gccgaagtgc cttgagagcg gcacagtggt gctggagcgc
     5761 ggcacggtcg agcagttgca taggttcttc gattgcttcg atcgcccagc gcggcgtatg
     5821 cccgcgctta ctacgcgata gacgcagcgt accttccagc tactcagcgc acatcggcac
     5881 agcgcagcag cggcggcaca gcggcacagc ggcacagcgc agcagcgcaa cgacacttcg
     5941 tgcactgacg catcgcgaag cgaaaccaac aagggaggag acatggaaaa aatctggttg
     6001 aagtcctatc caccgggcgt gcccgccgag atcgacgcta cgcagttcaa ctccgttgcg
     6061 gacttgctcg agcaaagctt ccagaagcat gcggcagagc gcgcgttcgt ctgcatgggc
     6121 tgcgagttga cctacggtga actcgatgcg aagtcgcgtc atcttgcagc gtggtttcaa
     6181 cggctaggtc tcgcgcgcgg cgcgcgcgtc gccgtgatgc tgccgaacgt gttgcagtat
     6241 ccggtggcca tggcggcgat tctgcgtgcc ggctacgtgc tggtgaacgt caacccgctg
     6301 tacacgccgc gcgaactggg gcatcagttg aacgacagcg gcgcgcaagc gatcgtgctg
     6361 ctcgatacgt tttctcaaac actggaagcg gtagaggcgc agacttcggt caagcatgtc
     6421 gtgctgacct cgatcggcga gatgcttggc gtgggcagcc ccgagatcgc ggccgatgtg
     6481 ccgcccactt cgcgcattgc attgaacgat gcgatcgcac gcggcgcgga cgcgggcttt
     6541 acaccggtcg cgctcacgcg tggcgacgtg gcggtgctgc aatacacggg cggcaccacg
     6601 ggcgtttcga aaggcgccac gctgttgcac ggcaacctga tcgccaacgt cttgcaatcg
     6661 gagttgtggc gcgagccggt ctatcgcgga cgcaccgaca tcaagcaggc catcactgtg
     6721 gcggcgctgc cgctgtatca catcttcgga ctgacggtct ccggcttgct gacgatgcgc
     6781 tgcggcggca tgggcattct gattccgaat ccgcgcgacc tggccggcct gatcaaagcc
     6841 ctgcgaggct acgcgatcaa ttcgttcccc ggcgtgaaca cgatgtacaa cgcgctgctg
     6901 aacgaacccg acttcaaaac gctcgacttc tccaagctgg tgcaatcgaa cggcggcggc
     6961 atggcggtgc agcaagccgt cgcgcaacgc tggcaagcgc tcaccggcgt gccgatcgtc
     7021 gaaggttatg ggctgtcgga aacctcgccg tgcgccacca ccaatctgcc gacgtccacc
     7081 gcgttcaccg ggacggtcgg cttgccgttg ccgtccacgg atatctcgat tcgcgacgac
     7141 gccggcaacg aagtgccgct cggagaacgc ggcgaaatct gcattcgcgg gccgcaggtg
     7201 atggctggct actggaaccg tcccgacgaa accgcgaggg cgatgacgcc ggacggcttc
     7261 ttcaaatccg gcgacatcgg cgtgatgacg gaagaaggct tcgtgaagat tgtcgatcgc
     7321 aagaaagaca tgattctggt gtcgggcttc aacgtctatc cgaatgaaat cgaggatgtg
     7381 gtggcgaagc acccgggtgt gttcgaagtg gtggcggtcg gtgtgccgga cgcggaggcg
     7441 ggcgaagtgg tgaagcttta cgtcgtgaag aaagacccgg cgctcaccga ggcggacatt
     7501 cttgcatttt gcaaggaaca actgacgggc tacaagcgcc ccaagatcat cgaatttcgc
     7561 agcgaactgc cgaaaaccaa cgtcggcaag attctgcgac gcgcattgcg cgacgaattg
     7621 aagcactctg ggtgaagcgg gcgtaccccg cgcttgccgg cgtggggcac gccctgcagc
     7681 gttgcgcgat tccgggtaca tttacgtcct cgacatcacg cactagggag tacacgatgc
     7741 gcctgcttca caccatgctc cgggtcggcg acctggaccg ttcgattgcg ttctacaacg
     7801 acttgctcgg catgaaattg ctgcgccgcc aggactatcc ggaaggcaag ttcaccctcg
     7861 ccttcgtcgg ctatgaagac gaacgcgacg gcgccgtcct cgaattgacc cacaactggg
     7921 acacgccgtc gtacgatctc ggcaacggct tcgggcacct cgccgtcgaa gtcgacgacg
     7981 cgtatgccgc ttgcgacaag atcaaggcac aaggcggcaa ggtcgtgcgc gaagccggcc
     8041 caatgaaaca cggcaccacc gtgatcgcct tcgtcgaaga tccggacggc tacaagatcg
     8101 agttcatcca gaagaaagcg tgacgtaagc ggcggggcga cgacaagcaa tgaataacag
     8161 caatccctac ttcaaagaac ttggcgacat ccacgccgaa atccaggcgc tgttcgacgg
     8221 ctccgcggat cacggcgtgc tggatcgcat catggcgcgc ttcgctccgc aattcacgct
     8281 ggtcatgaca tccggtatcg cactggacca cgcgggcgtt cgcgagattt tcgcgaagtt
     8341 gaatggcgcg cgtccgggct cgcaaatctc gatccgcgat atggagatcg tgtccgagta
     8401 tccatcgggc gcggtggtga aatatcgtga gtatcagcgc gacaaggcgg gcaatgcgaa
     8461 cgtgcggcgc tcgacagcgg tgatggatct ggatgcgcac ggcaaggtga cgtggcgcca
     8521 tctgcaggaa accatctgca cggcatgagc cttcttgggg ctcggaagac cggcgcttcg
     8581 ggtcgaagcg ccgccttcaa atcttcatgc atggtctccg gtccgggcta ttgaccgaaa
     8641 ttctcaaaga ccggttgcaa cggccggaaa acgccaaggc cgcaggatcc gacggctcta
     8701 aagcgtcggc agcaattccg gcggatgcga cttcaacgtc tggcgcgcct cgcggaattc
     8761 cggaaaaatc gattcgaccg tctgccagaa gcgcgggctg tgattcatct cgcgcagatg
     8821 cgcgagttcg tgcgcgacga cgtaatcgat aatcgacagt gggaaatgga tcagccgcca
     8881 gttcagacga attttgccgt cgctcgaaca actgccccaa cgcgtggccg ccgaagaaag
     8941 tgcatatgcg cgatagttga cgccaagctt ctccgcgtag atcgcgaggc gctcgccgaa
     9001 caggcgtttc gcttcgcctt gcagccagcc ttgcacgcga tccttgatct gctgagggtc
     9061 ggcttgcaac ggcagcggca cttgcaatgc ggcttcctcc gcattgaacg cgagcatgcc
     9121 ttgcggtgaa ccgagcttca cgcgcaccgg ctggccgaga tacggcactt ccgcaccatc
     9181 tttccagtcg accttgggca acgcgcgctg ttcgacacgc gtctgccatt cgatcagctt
     9241 cgtaaaaatc cagcgctgct tttcggtgat cgcggtttcg atatcggcga gcgtgaccca
     9301 acgtggtgcg gtaatcatca ggccggtgct gtcgatcgca aagccgatcg agcggcgtgc
     9361 cgaacgcttg agcgcatagt gcagcgtgcg cgaaccgatg gtgaggctgc gcagcctggt
     9421 accgtccggg gcgagcggca cggcgggctg ctgcccgctc tgcgagcccg aaggcgcgga
     9481 gggtgttggg gacgacgacg tcgaccccgg ctcggcgaag agcgggagat cgagctgccg
     9541 gttatcgagc gccacagcgg gctgcgacgt aggagacttc tgcatcggat tcggcttcgc
     9601 gcaaatgcgc cttcgagggc gcgggcgcgt cagattggtg cggcggcgga gctgctatcc
     9661 gccgcgcggt acgcggtagg atcgatgcga cgcatttcag cttcgatcca ctgttcgacg
     9721 cgcgtgttca cttcatcggg cgtgagcccc gtggtctcga tcggcttgcc gatcgacact
     9781 gtgactatac ccgcatattt gagaaacgag ttacgaggcc acacacgtcc ggcgttgtgc
     9841 gcgatcggca cgacaggcgc gccggtggca atcgcgaaac gcgcgccgcc ggttttgtac
     9901 ttgccttgct tgccggtcgg cgtacgcgtg ccttccggaa acatgatcac ccaggcgcct
     9961 tccgccatgc gcgccttgcc ttgcctgatg accgattcga acgcgtactt gccttccttg
    10021 cggtcgatgt gaaccatctt cagcatgccg agcgcccagc cgaagaacgg cacatacagc
    10081 agctcgcgct tgaacacgta gcacagcggc cgcggcatca gcgccggaaa cgccagcgtt
    10141 tcccacgccg attgatgctt ggacagcagc acggccggac cgtcgggcaa attctcgaag
    10201 ccgtcgatcc ggtagcggat gccgttcagc cagcgcaccg tgtgaagcgt ggcgcggcac
    10261 cagccggccg ccatccagta gcgattatcg gcgcgcatga acggaaacgc gatgaagcac
    10321 gcggtcgcat acggcacggt gaacagcacg aagtagatca gcagcagcaa agaacgaatg
    10381 aagcgcatcg gggttgtctg tgaggggtgg ggacgcgctc gacgcgcatc actcgtgatc
    10441 gtcggagaga aaatcgagcg cgaacgcgcg caggtcatcg tgcacctggg tgcctgcggg
    10501 caggccgcca gctgcgagcg ttttgcgacc cttgccggtc aataccagat gcgtcggaaa
    10561 gccgagtgcc gcgcccgctt ccagatcgcg catcgcgtcg ccgactaccg gcgtatgctc
    10621 cggatcgact tcgaaacgtt cggtgatcat cttcagcatg ccgggcttcg gcttgcggca
    10681 ttcgcagtgg tcttgcgccg tatgcgggca gaagaacacc gcgtcgatac gtccgccgac
    10741 cgccgccgcc atgcgatgca ttttcagatg catcgcattg agcgcgttca tgtcgaagag
    10801 accgcggccg atgcccgact ggttcgtcgc gatcgccacg cgataacccg cctggttcag
    10861 ccgcgcgatc gcttcgagcg cgcccggcag ggcaacccat tcgtccggcg acttgatgaa
    10921 cgcgtcggaa tcgacgttga tcacgccgtc gcggtcgagg atcaccactt ttttggtcgg
    10981 catggggcag cgctcaggcg gcgagtcggg aaatgtcggc gacgcaattc atctgctgat
    11041 gcagcgcgcc gagcaaggcc agacggttgg cgcgcaacgc cggatcttcg gcgttgacca
    11101 tcacgtcgtt gaagaacgtg tcgaccggtt cgcgcaacgc ggcgagcgcg gtc
//
LOCUS       DATASH010000113         5497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2784372,
            whole genome shotgun sequence.
ACCESSION   DATASH010000113 DATASH010000000
VERSION     DATASH010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5497
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2784372"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..146)
                     /locus_tag="VHV99_03395"
     CDS             complement(<1..146)
                     /locus_tag="VHV99_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEX3634529.1"
                     /translation="MRSIRRQLLFWLLALVLLGVGIAGWLIYRQALAEANELFDYQLE
                     QIAAA"
     gene            complement(151..813)
                     /locus_tag="VHV99_03400"
     CDS             complement(151..813)
                     /locus_tag="VHV99_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3634530.1"
                     /translation="MRILLVEDDRMIAEGVRKALRGEGFAVDWVEDGEAALSAAAGQP
                     YDLVLLDLGLPKRDGLEVLRTLRAKGHATPVLILTARDAVADRVKGLDAGADDYLVKP
                     FDLDELGARMRALIRRQSGRSDSTIRHGNLTLDPASHQVTLDGAPVALSAREFALLEA
                     LIARPGAVLSKSQLEEKMYGWGEEIGSNTVEVYIHALRKKLGADLIRNVRGLGYMIAK
                     DA"
     gene            1130..2779
                     /gene="dacB"
                     /locus_tag="VHV99_03405"
     CDS             1130..2779
                     /gene="dacB"
                     /locus_tag="VHV99_03405"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEX3634531.1"
                     /translation="MTLAFLSSLARCVALPPPSPARARALPRRFAPRTAALLLACAAL
                     GCGTLLPLTAQARVKATHPSVNVTTVLPQPVMVGLQRAHVPLSSISVVVEKVGDRTPI
                     LALNAGKPMMPASTMKLVTTYSGLSILGPDYRWRTSAYADGTVDANGVLHGNLYIQGT
                     GDPKLVPEELIDLVQKIHKAGISGIDGALVLDKRYFDASTRDLPPFDDDATAPYNVGP
                     DPLLYAFKSLSFTLTPSPDGSVAIDVLPALSQLQIDNQMHAVSGPCRGDAASATPTVT
                     PQPNGTVVASFAGDYSMRCGPRTINVAVLDHSAFFAGGFLALWQQTGGTFSGATHEGA
                     VPLGARLVATHQGPMLSDIVRDINKFSNNTMARNLFLTIGATEEKPPATPAKSARAIE
                     AFLRRDSIDMEYLTLDNGSGLSRDERVTALALADLLQRANASPVAQVFVESLPIAGVD
                     GTMRNRLINQGAGGNAHIKTGTLRDVRAIAGYVASADGNSYVVVSLINDPHSEAARAA
                     HDALLEWVYQGPSQGFTKVSDPVTEPRGSKPRKNPHKRSTH"
     gene            2985..3935
                     /locus_tag="VHV99_03410"
     CDS             2985..3935
                     /locus_tag="VHV99_03410"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX3634532.1"
                     /translation="MQFDAELLLLAMAPVFLACIGWEAWHLRRTRPGAQLYSWRDTFC
                     NAALALMQQAADKLAWLAIIPVYAFFYDHYRVTTWQASWASFVVLFVAQDLLYYVFHR
                     CSHRVRWLWAAHVVHHSSERMNFSTAFRQSLMYPIAGMWLFWIPLAMLGFPPRQIVAI
                     VLINLGFQFFVHTQAIGKLGWLEYVFNTPSIHRVHHARNDRYIDRNYAGVLAIWDRLF
                     GSFVEEDPHDAPVYGIVEPLHTYNPLKATFHEWASMAADFVSVRGWRNKWRALFAPPA
                     WAAAYHARRTTGASVDIAAAGGDMSVEENQTAAFQTPRRP"
     gene            4031..5239
                     /locus_tag="VHV99_03415"
     CDS             4031..5239
                     /locus_tag="VHV99_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX3634533.1"
                     /translation="MKQTASKKLKFVRVTQIAIASAAFALLAACGGGSDNNSSSSTPT
                     GGVKLQVVSFGDSLSDVGTYAPVIQLSFGGGRFTTNPGEVWTQKVAEYYGGTLSAAYL
                     GGFGQPLVAAGGYGYAQGGSRVDQPQGEGYAPNNMAATTIPVTQQITEYLGAHSSFNS
                     NQLVLMNGGANDIFIQAQAVSDAGTAAATQVLLSGGTPAAAQAAGQAASLAATPAALQ
                     AIGVAAGKFIGAVQQVIAAGATHVVVSDVPDIKSTPQGVQSGAQGQQLFDALTLTFNG
                     ALAQAFASNKQVIYVSAYKWLDTSLASYQQLGFTVSNTGTACNLNAMGQAATTYATQN
                     PLVLQPGQTAASYGSQFQSSLFCSPQTYTVAGADQTYMFADLVHPSSHLQALFAQTVE
                     QAIAATGLGK"
BASE COUNT          984 a         1826 c         1710 g          977 t
ORIGIN      
        1 gccgcggcga tctgctccaa ctggtaatcg aacagttcgt tggcttcggc aagcgcctgc
       61 cggtagatca gccaacccgc aatgccgacg ccgagcaaca ccagcgccag cagccagaac
      121 agcaattgac gacgaatcga acgcatcggc tcaggcgtcc ttcgcgatca tgtagccaag
      181 accgcgcacg ttgcggatca ggtccgcgcc gagcttcttg cgcagtgcgt gaatatagac
      241 ctcgacggta ttgctgccga tctcctcgcc ccagccgtac attttttcct cgagctggct
      301 cttcgagagc accgcgccag gacgcgcgat cagtgcttcg agcagcgcga attcacgcgc
      361 cgacaacgcg accggcgcgc catcgagcgt gacctgatgc gaggccggat cgagcgtgag
      421 gttgccgtgg cgaatcgtcg aatcgctgcg gcccgattgg cggcggatca gcgcgcgcat
      481 gcgggcgccg agttcatcga gatcgaaagg tttgacaagg taatcgtcgg cgccggcgtc
      541 gaggcccttg acgcggtcgg cgaccgcgtc gcgcgccgtg aggatcagca ccggcgtggc
      601 gtggcctttg gcgcgcagcg tacgcagcac ctcgaggccg tcgcgtttgg gcaggccgag
      661 gtcgagcaac accagatcgt aaggttggcc ggctgccgcg ctgagcgccg cttcgccgtc
      721 ttccacccaa tcgaccgcga agccttcgcc gcgtagcgcc ttgcgcacgc cttcggcgat
      781 catccggtcg tcttcgacta gcaatatgcg catgttggta cggtcctgaa aacattcgat
      841 gatgccgcat tctagcgccc agcccggcat gcgcgccgcg cgatgctttt tcaccgcagc
      901 ctcgcggcat gtctctacaa tgggcgcttt gcgtcgcgcg gctcgattca tctgcatgcc
      961 ccgctccgta tgcggagtgc gtgaatccgc cgccgcgtgt gcatgaccgc ccgatgccgc
     1021 tctcgcgaac gcgcaacgaa accgctgccc gcacgcgtcg cgcgatgcat cgacacaccc
     1081 tggattgccg cgtaccacgc gccccgaaat cttgcttcct cgcctgtcca tgacgctcgc
     1141 ttttttgtct tctctcgcac gctgtgtggc gctgcctcca ccctcccctg ctcgcgcccg
     1201 cgccctgccg cgccgcttcg cgccgcgcac ggcggccttg ctgctcgcct gtgccgcgct
     1261 cggttgcggc acgctgctgc cgctcaccgc acaggctcgc gtcaaggcca cgcatccgag
     1321 cgtcaatgtc acgaccgtcc tgccgcaacc ggtgatggtc ggcctgcagc gcgcccatgt
     1381 gcccttgtcg tcgatcagcg tggtagtcga aaaagtcggc gaccgcacgc cgatcctcgc
     1441 gctgaatgcc ggcaagccga tgatgccggc ctcgacgatg aagctcgtca ccacttactc
     1501 cggcctgtcg attctcggcc cggactatcg ctggcgcacg agcgcgtatg cggacggcac
     1561 ggtagatgcc aacggcgtgc tgcacggcaa tctatacatt cagggtacgg gcgacccgaa
     1621 gctcgtgccc gaagaactga tcgacctcgt gcagaagatc cacaaggcgg gcatcagcgg
     1681 tatcgacggc gcgctggtgc tcgacaagcg ttacttcgat gcctcgacgc gcgacctgcc
     1741 gccgttcgac gacgacgcca ccgcgccgta caacgtcggc cccgacccac tgctgtatgc
     1801 cttcaaatcg ctgtcgttca cgctgacacc ttccccggac ggcagcgtcg ccatcgacgt
     1861 gctgcccgcg ctctcgcagt tgcagatcga caatcagatg cacgcagtga gcggcccatg
     1921 ccgtggcgac gccgcctccg ccacacccac cgtgacgccg caacccaacg gcaccgtggt
     1981 ggcgtcgttc gccggcgact actcgatgcg ctgcggcccg cgcacgatca acgtcgcggt
     2041 gctcgatcac tccgcgttct tcgcgggcgg cttcctcgcg ctgtggcagc aaaccggcgg
     2101 cacgttcagc ggcgcgaccc acgaaggcgc ggtgccgctc ggcgcgcgcc tcgtcgccac
     2161 ccaccagggg ccgatgctgt ccgacattgt tcgcgacatc aacaagttca gtaacaacac
     2221 aatggcgcgc aacctgttcc tgacgatcgg cgcgacggaa gaaaaaccgc ccgccacgcc
     2281 cgccaaatcg gcgcgcgcga tcgaggcgtt tctgcgccgc gacagcatcg acatggaata
     2341 cctgacgctc gacaacggtt cgggtctctc gcgcgacgag cgcgtcaccg cgctcgcgct
     2401 cgccgatctg ctgcagcggg ccaacgcgag cccggtcgct caggtgttcg tggagtcgct
     2461 gccgatcgcc ggcgtggacg gcacgatgcg caaccgcctg atcaaccagg gcgccggcgg
     2521 caacgcgcac atcaagaccg gcaccctgcg cgacgtgcgg gcgatcgccg gctatgtggc
     2581 ttcggcggac ggcaacagtt atgtggtggt gagcctgatc aacgacccgc attccgaagc
     2641 cgcgcgcgcc gcacacgacg ccttgctcga atgggtatac cagggaccgt cccagggatt
     2701 cacgaaagtc tccgacccgg tcaccgaacc gcgcggcagc aagcccagga aaaacccgca
     2761 caagcgcagc acccattgag gattccttct atagaagccg cgcgccgcaa cgcgcccaag
     2821 cgtgtacgct gtcgggcagt gtttgaggcg cgcgtaaaac gcaacgcgta gcgcacgagc
     2881 cgcgcagccc cgcgtaacca ttagggtcgc gtggcccatt caaacgaaaa cgacaacgtc
     2941 cgttgcggca aacgcagcgg ccagggacca gggaggaaga cataatgcaa ttcgatgccg
     3001 aattgctgct gctcgccatg gcgccggtct ttctcgcatg cattggctgg gaggcgtggc
     3061 acctgcggcg cacgcgcccc ggcgcgcagc tttatagctg gcgcgacacg ttctgcaacg
     3121 ccgcactcgc gctgatgcag caagccgccg acaagctcgc gtggctcgcg atcatccccg
     3181 tgtatgcctt tttctacgat cactatcgcg tcaccacgtg gcaggcgagt tgggcgtcgt
     3241 ttgtcgtgct gttcgtcgct caggatctgc tctattacgt gttccaccgt tgcagccatc
     3301 gcgtgcgctg gctgtgggcc gcgcatgtcg tgcatcactc gtcggagcgg atgaatttct
     3361 cgaccgcatt ccgccagagc ctgatgtacc cgattgccgg catgtggctg ttctggatcc
     3421 cgctcgccat gctcggcttt ccgcccaggc agatcgtcgc gatcgtgctg atcaacctcg
     3481 gcttccagtt tttcgttcat acccaggcga tcggcaaact cggctggctc gaatacgtgt
     3541 tcaacacgcc gtcgattcac cgcgtgcatc acgcgcgcaa cgaccgctat atcgaccgca
     3601 actatgccgg cgtgctggcg atctgggatc gcctgttcgg cagctttgtc gaggaagatc
     3661 cgcacgatgc gccggtgtat ggcatcgtcg agccgctcca cacgtataac ccgctgaaag
     3721 cgacgtttca tgaatgggcg tcgatggccg ccgatttcgt gagcgtgcga ggctggcgca
     3781 acaagtggcg cgcgctgttc gcaccacccg cgtgggccgc ggcgtatcac gcccggcgca
     3841 ccaccggcgc gagcgtcgat atcgcggcag cgggcggcga tatgagcgtt gaagaaaacc
     3901 aaacggccgc gtttcaaaca ccgcgcaggc cttagaagat tctgtaagcg tgtcgtcacg
     3961 ccgcggacgc gggggacgcc gcctgcaata catggcacaa aggccaccca tacgaggaga
     4021 tgaagttaac atgaagcaaa cagcatcgaa gaaactcaag ttcgtacgcg tcacgcagat
     4081 cgccatcgcc agcgcggcgt ttgcgctgct cgcggcttgt ggtggcggca gcgataacaa
     4141 cagttcgagc agcacgccga cgggcggcgt gaaattgcag gtcgtatcgt tcggcgacag
     4201 tttgtcggat gtcggcacgt atgcgccggt gattcagttg agcttcggcg gcggccgctt
     4261 cacgaccaac ccgggcgaag tgtggacgca gaaggttgcc gaatactatg gcggcacgtt
     4321 gagcgccgcg tatctgggcg gcttcggtca accgctggta gcggcgggcg gatatggcta
     4381 cgcgcaaggc ggctcgcgcg tagatcagcc gcaaggcgaa ggttacgcgc cgaacaacat
     4441 ggcagccacg acgattccgg tcacgcagca gatcacggaa tatctgggcg cgcacagcag
     4501 cttcaattcg aaccagctcg tgctgatgaa cggcggcgcg aacgacattt tcattcaggc
     4561 gcaagcggtt tcggacgccg gcaccgcggc ggcaacgcaa gtcctgctga gcggcggaac
     4621 gcctgcggcg gcgcaagctg cgggccaagc cgcctctctg gcggcgaccc ccgcagcgct
     4681 tcaggcgatc ggcgtggcgg cgggcaagtt catcggcgcg gttcagcagg tcatcgcggc
     4741 gggcgcgacc catgtggtcg tgtcggatgt gccggacatc aagtccacgc cgcaaggtgt
     4801 gcagagcggt gcgcaaggtc agcaactgtt cgatgcgctg accctgacgt tcaacggcgc
     4861 gctggcacaa gcgtttgctt cgaacaagca ggtcatttat gtgagcgcgt ataagtggct
     4921 ggacacctcg ctggcgtcct accagcagct gggcttcacg gtgtcgaaca cgggcacggc
     4981 gtgtaacctg aacgcgatgg ggcaagcggc cacgacctat gccacgcaga acccgctcgt
     5041 gctccagcct gggcagacgg ctgcgtctta cggcagccag ttccagtcgt cgctgttttg
     5101 ctcgccgcaa acttataccg tagccggcgc ggatcagacg tacatgtttg cggatctggt
     5161 ccacccgagc tcgcacttgc aggcgttgtt cgcgcagacg gtcgagcagg caatcgctgc
     5221 gaccgggctg gggaaataag tcgagggttg ccgcgtagcg gcagcaggat gcgagttctg
     5281 cgaacgcctg gcttgctggt caagccaggc gttttttatt gcccgcgcgg cacttctgat
     5341 taatcgccag cggcgttagc gtgtcgtgat ttgtttgccc acgcggcgta cctcgcatct
     5401 ccggacctcg cggcggcctg actgcggcgc tcgatatcta tttgtcgctg cgcgcgacct
     5461 tagcaccacg caggcaaaaa aatcacactt caacctt
//
LOCUS       DATASH010000114         4687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1795725,
            whole genome shotgun sequence.
ACCESSION   DATASH010000114 DATASH010000000
VERSION     DATASH010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4687
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1795725"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1165
                     /locus_tag="VHV99_03420"
     CDS             <1..1165
                     /locus_tag="VHV99_03420"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012400680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HEX3634534.1"
                     /translation="LVIRGRSLLPIVQGGMGVGISAHRLAGSVAREGALGTIASIDLR
                     HHHRDLIELCRQAPDRATLEQANLTALAREIHAAKALSDGRGMIAVNVMKAVNAQADY
                     VRVACESGADAIVMGAGLPLDLPDMTQGHDIALIPILSDSRGVALVLKKWLKKGRLPD
                     AVVIEHPAHAGGHLGVTNLADLHDPRFDFLRVLEELDPVFISLGLSRQDVPLIVAGGI
                     NSHEAVRELLTAGASGVQLGTPFAVTEEGDAHPNFKRVLADATPDDIVEFVSVTGLPA
                     RAVKTPWLMRYLRHEAKIREKIGALKHVCPTALECLSVCGWRDGVERFGHFCIDTRLA
                     AALRGDVANGLFFRGREALPFGTAIRSVHDLIELLLTGLTRPAVAGRWAFSLA"
     gene            1236..1895
                     /locus_tag="VHV99_03425"
     CDS             1236..1895
                     /locus_tag="VHV99_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HEX3634535.1"
                     /translation="MKHSLRNRIKAAAVASCLLCAGVPLAAHAQAAGNDQMNGIHAGD
                     ILVRLRAISIMPDVGTSRSLSALNVGVNNAIVPELDLTYMIRDYLGVELILGTSRHQL
                     SSNLGNLGGVNVLPPTLLLQYHFNHAGRIRPYVGAGLNYTLFYNNGLNAGGQPISITN
                     HSFGPALQAGVDVQVTKSLFVNADIKKIWMHTDASLGAQSLGRLNIDPVVVGFGVGTR
                     F"
     gene            complement(2025..2228)
                     /locus_tag="VHV99_03430"
     CDS             complement(2025..2228)
                     /locus_tag="VHV99_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006404745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HEX3634536.1"
                     /translation="MASNLHDLPDSPCIGVCSTLFDEVCKGCGRTATEVSNWVFLSDE
                     EKRAVWVRIEQDGTAMRFKNDKL"
     gene            complement(2356..2535)
                     /locus_tag="VHV99_03435"
                     /pseudo
     CDS             complement(2356..2535)
                     /locus_tag="VHV99_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002442.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
     gene            complement(2774..4078)
                     /locus_tag="VHV99_03440"
     CDS             complement(2774..4078)
                     /locus_tag="VHV99_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEX3634537.1"
                     /translation="MNTNPSALRPNRRRRLPEDGPARPAHWFSFVGAGALIAVGYIDP
                     GNWATALGAGAGYGYRLLGMVLLASLMAMLLQWLSSRLGVVTGRDLAQICRERSSRRV
                     TLFLWLTSEVAIIACDVAEVVGSAVALQLLLGVSLTVGVLMSAVCTFALLALQQKGGR
                     KLEFVIASLIGFVGLCFVIELALARPDWHAALAGTAPSLDLLRNAGMVWLAAGIVGAT
                     VMPHNLYLHSALVKHHAPEGSDVQIKAALQVVNLDTFGSLSFAFVINAALLIVAAAVF
                     YASGHRDVTDLADAHRLLAPLVGTHWAAILFAAALLACGLSATVTGTLAGQAVMEGFL
                     RLRLPRWKRALLTRALAIGPALLAVALFGQHGSNQLLVASQVVLSLQLPLAVVPLIRY
                     TSDAGLMRGWHVRGVPLVLAWLSAVFIILLNVALLWQLAFGG"
     gene            4577..>4687
                     /locus_tag="VHV99_03445"
     CDS             4577..>4687
                     /locus_tag="VHV99_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004266745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX3634538.1"
                     /translation="MKRRVVGQVAALVLCATPWLTAAAKDTQLNVYNWSDY"
BASE COUNT          938 a         1465 c         1496 g          788 t
ORIGIN      
        1 gctcgttatc cgcggccgct cgctgctgcc gatcgtacaa ggcggcatgg gcgtcggtat
       61 ctcggcgcac cggctggccg gcagcgtcgc ccgcgaaggc gcactcggca ccatcgccag
      121 catcgatctg cgtcatcatc atcgcgatct gatcgaactc tgtcggcagg cgcctgatcg
      181 ggctacgctc gaacaggcga accttaccgc gctggcccgt gaaatccacg ctgccaaagc
      241 gctcagcgat ggccgcggca tgatcgccgt caacgtgatg aaagcagtca atgcgcaagc
      301 agattatgtg cgcgtcgcct gcgaaagcgg cgccgacgcg atcgtcatgg gcgcgggcct
      361 gccgctcgac ttgccggaca tgacgcaagg ccacgacatc gcgctgatcc cgatcctgtc
      421 ggatagccgc ggcgtcgcac tggtgctgaa gaagtggctg aagaagggcc gcttgcccga
      481 cgccgtcgtg atcgaacatc cggcccacgc aggtggccat ctcggcgtga caaacctcgc
      541 cgatctgcac gacccgcgct tcgatttcct gcgtgtcctc gaggaactcg atccggtatt
      601 catctcgctc ggtttgagtc gccaggatgt gcccttgatc gtcgcgggcg gcatcaatag
      661 tcacgaagcc gtgcgcgagt tactgaccgc gggtgctagc ggtgtgcagc tcggcacacc
      721 gttcgccgtc accgaagaag gcgacgcgca tccgaacttc aagcgcgtgc tggccgacgc
      781 gacgcccgac gacatcgtcg aatttgtcag cgtgacgggg ctgccggcgc gcgccgtgaa
      841 gacgccgtgg ctgatgcgct atctgcgcca cgaagcgaaa atccgcgaga agatcggcgc
      901 gctcaaacat gtatgtccga ccgcgctcga atgtctcagc gtctgcgggt ggcgcgatgg
      961 tgtcgagagg ttcggccact tctgcatcga cacgcggctc gccgcggcat tgcgcggcga
     1021 cgtggccaac ggtctgttct ttcgcggccg cgaagcgttg ccgttcggca cggctattcg
     1081 cagcgtccac gacctgatcg aactgctcct gaccggcctg acgcgaccgg ctgtcgcagg
     1141 acgatgggcg ttctcgcttg cttgatgcgg gtcaacgtga agcctcacga gaacttcgac
     1201 aatccccgca cgcaaacaac tactcgggga agcacatgaa gcactcgctc aggaacagga
     1261 tcaaggccgc tgccgtcgcg tcgtgtctgc tatgcgcagg cgtgccgctg gcggcgcacg
     1321 ctcaggctgc cggcaacgac cagatgaacg gcatacacgc cggcgacata ctggtgcgtc
     1381 tgcgcgcaat cagcatcatg ccggacgtgg gaaccagccg gtcgctgtcc gcgctcaatg
     1441 tgggcgtcaa caacgccatc gtgccggaac tggatctgac ctacatgatc cgcgattacc
     1501 tcggcgtcga gctgattctc ggcacgtcgc gccatcaatt gagttcgaac ctcggcaatc
     1561 tcggcggcgt gaacgtgttg ccgcccacgc tattgctgca atatcacttc aaccacgcag
     1621 ggcgtatccg tccgtatgtc ggtgcgggtc tgaattacac gttgttctat aacaatggtc
     1681 tgaacgcagg tggtcagccg atctcgatca ccaaccatag ctttggcccc gccttgcagg
     1741 caggtgtgga cgtgcaggtg accaagtcgc tgttcgtcaa cgcggacatc aagaaaatct
     1801 ggatgcacac cgacgcctcg cttggcgctc agtcgctcgg ccggctgaat atcgatccgg
     1861 tggtggtcgg gttcggtgtc ggtacgcggt tctgatttac gcgctcgagc cgctgcgctc
     1921 atgatgcaaa aacgccggaa tttccggctt ttttttattc agttccaatc gggagacacg
     1981 ctcgccgaca gccgggccta tacgcgtccc gcggcgcgat ccggctatag cttgtcgttc
     2041 ttgaagcgca tggccgtgcc gtcctgctcg atgcgtaccc acacggcgcg cttttcctcg
     2101 tcgctcagga acacccagtt agatacttcg gtggcggtgc ggccgcagcc tttgcagacc
     2161 tcgtcgaaca gcgtggaaca gacaccgata cacgggctgt cgggaagatc gtggagattt
     2221 gaagccatcg gaaaccttgg agcgcggaag cgaagttccg ctatgttagc cgatgcggcg
     2281 gccgcgcctc ggcgcggcca acaccatggc caggaaccat ggacgattgg cagcatccgg
     2341 cggtgctttg gcaggcacgt agtagaacaa ctgcggggag taggtcgtgt cgcggaacgg
     2401 ccgcgagtcg gcggacagat cccagatcga ggcctgcgta taggcgaagt acaggttgtc
     2461 gaggaaggcc ttcgagcgca gatcgtccgg catcaggatg cggtatttga agcacagttg
     2521 cagccgggcg ttcgtgggcg agacggcaag acggcaagac ggcaagacgg caagacggca
     2581 agacggcaag acggcaagac ggcaagacag caagacagca agacagcaag acagcaagac
     2641 agcaagacag caagacagca agacagcagg acagcaagac agcggcatcg cccgtcatcg
     2701 ggaaatacta agagaatact tacaaaccgg cttcattcgc aaacgcaatg catggagcgg
     2761 ccggcaaacc acttcatccg ccgaacgcca actgccatag cagcgccaca ttcagcagga
     2821 tgatgaacac cgccgacagc cacgccagca cgagcggcac gccgcgcacg tgccagccgc
     2881 gcatcaggcc ggcgtccgac gtgtagcgga tcaacggcac caccgcgagc ggcaattgaa
     2941 gactcagcac cacctggctt gcgaccagca gctgattgga gccgtgctgg ccgaatagcg
     3001 cgaccgccag aagcgctggg ccgattgcca gcgcgcgggt gagcagggca cgcttccagc
     3061 gcggcaggcg caggcgcaga aaaccttcca tcaccgcctg acctgccagg gtgcccgtga
     3121 cggtcgcgct caggccgcag gcgagcagcg ccgccgcgaa caggatggcc gcccaatgcg
     3181 tcccgacgag cggcgcaagc aaccgatggg cgtcagcgag atcggtgacg tcgcgatggc
     3241 cgctcgcgta gaacaccgcc gccgcgacaa tcagtagcgc cgcgttgatc acgaacgcaa
     3301 atgacagcga gccgaacgta tccagattca cgacctgcag cgcggccttg atctgcacgt
     3361 cgctgccttc cggcgcgtgg tgcttgacga gcgcggagtg cagatacagg ttgtgcggca
     3421 tcaccgtcgc gccgacgatg ccggccgcga gccacaccat gcccgcattg cgcagcagat
     3481 cgaggctcgg cgcggtgccg gcgagcgcgg cgtgccagtc gggccgcgcc agtgccagtt
     3541 cgatcacgaa gcacagaccg acaaaaccga tcagcgatgc gatcacgaat tcgagcttgc
     3601 ggccgccttt ctgctggagc gccaagagcg cgaaggtgca gaccgccgac atcagcacgc
     3661 cgaccgtcag tgatacgccg agtagcaact gcaacgcaac ggcgctgccg accacctcgg
     3721 cgacgtcgca ggcgatgatc gcaacctcgc tcgtcaacca taggaaaagc gtgacgcggc
     3781 ggctcgagcg ttcgcggcag atctgcgcga gatcgcgccc ggtgaccaca cccagccgcg
     3841 acgacagcca ctgcagcagc atcgccatca ggcttgccag cagcaccatg cccagcagcc
     3901 ggtagccgta gcccgcaccg gcgccgagcg cggtcgccca gttgcccgga tcgatatagc
     3961 cgaccgcgat cagcgccccc gcgccgacga acgaaaacca gtgcgccgga cgtgccggcc
     4021 cgtcttccgg cagacgccga cggcggttcg gtcgcaacgc ggacggattc gtgttcatcg
     4081 atgcctggga ggtttgagat tgcagccact cgcaagcgac atgcccgtgg cgaactggca
     4141 cacgacgcag tgaggccaag gcatgccagc ggggtgccgt ttttcaagtc ggtcagagct
     4201 attgtgcacc gtagcgcggc cagcggcttg gctcgtatcg aggttaaccg taaggcatga
     4261 gattcgcgga acgcgtcgct ggcagccacc ctgatgcgga tcgggcgcgg tgccgccgct
     4321 gcaagttgac agtcagctaa cactgagtcg ccactggcgt tttttttggg tggacacgcg
     4381 ccgataaccc gctaaaattg cgcccatttt gtagcggcgc ggcttacgct gtaccgtttg
     4441 cgcgacagct cggcattttt tttggtgcgc gcaataatta atggtagttt tcgttttttt
     4501 caggggagcc gcgtgcggta tgccgcggcg tgagtgagga atggccggct cgagccggtc
     4561 aggatcggag aacggaatga aaagacgggt agtggggcag gtggcggcgc tggtcttgtg
     4621 cgcgacgccg tggttgacgg ccgctgcgaa agatacgcag ctgaacgtgt ataactggtc
     4681 cgattac
//
LOCUS       DATASH010000115         2743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3378376,
            whole genome shotgun sequence.
ACCESSION   DATASH010000115 DATASH010000000
VERSION     DATASH010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3378376"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="VHV99_03450"
     CDS             complement(<1..558)
                     /locus_tag="VHV99_03450"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HEX3634539.1"
                     /translation="MNFVTRKSLHRRTLLKGLGAGVALPLLDAMIPALTAQTRSAQTK
                     SAPPRLGFVYVSHGIIFSQWKPQQTGRNFELSPNLQPLAGVRDHVNVFTNLSHLEADT
                     KGDGSGDHNRAAAAWLTGVHAYDRTRPGAEIRLATSVDQIAARQLGRDTQVRSVEVTT
                     DAPSQGACDSGDCFYVNTVSWRNETA"
     gene            complement(583..>2743)
                     /locus_tag="VHV99_03455"
     CDS             complement(583..>2743)
                     /locus_tag="VHV99_03455"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HEX3634540.1"
                     /translation="ARLDHMDTSAETWEAVLGKLRAGAMPPPGAPRPEAAVYKRLSEY
                     LTGQLAANAAAHPNPGGVLPVHRLTRTEYKNSIRDLLALSNLPKEMDYTTLLPADNVS
                     SGFDNLADTLFVSPVAMERYLDAARKISQLATGDPAIGVLVNLHQTPIRQRQEARDEQ
                     LPFGTRGGMAVHSYFPLDGEYEFQIAVSGLQREAHELELSIDGARQSLVSLTGRRADD
                     DDPASPAKQQFRATVKAGPRTVDVAFIERSAALSESFLRPPGRTRGALPSVVSVTITG
                     PYNATGPGDTPSRRLIFSCHPHTAAEEPACAREIVSNLMRRAYRRTVSDGDLAPAMKF
                     YAAGRADRDFDCGIERALERMLVSPQFLFRVEGEPASARAGAPYPVSDTELASRLSFF
                     LWSSIPDDELLNAAASGKLREPDVLRAQVARMLADPRAETLVTNFAAQWLFLHDVESK
                     DPDLYLFRDFDDGLKPAFERETELFLDSILRSDRSVLDLLTANYTYVNERLAKHYGIP
                     NVTGSHFRKIELPPGSPRAGLLGQGSILAPTSYSTRTSVVLRGKYVLQNLLNMPPPAP
                     PPNIPALKTESGAGQSLSMREAMLQHRANPACAGCHAKMDPIGFALENFDAVGRWRDE
                     DGGKPIDVVSTLPDGTRLDGVDGVRQLLLREPGLFVEAMTSKLLMYAVGRNVQYYDGP
                     LIRAIARESQKRNYTFASLVEGVVTSDQFQMRVKRTR"
BASE COUNT          517 a          788 c          896 g          542 t
ORIGIN      
        1 agcggtttcg tttcgccagg aaacggtgtt gacgtaaaag cagtcccctg aatcgcaggc
       61 gccctgcgaa ggcgcgtctg tagtgacctc gacggagcgc acctgagtat ctcggccgag
      121 ttgtctggcg gcgatctgat caaccgaagt ggccagccgg atttccgccc cgggacgcgt
      181 gcgatcgtaa gcgtgaactc cagtcagcca ggcagcggcg gcacgattgt gatcgcccga
      241 tccgtcgccc ttagtatcgg cttcgaggtg cgagaggttg gtgaatacgt tcacgtgatc
      301 gcgcaccccg gccagcggct gaaggtttgg cgacagctcg aagttcctgc ctgtctgctg
      361 cggtttccac tgcgaaaaaa ttatgccgtg cgatacatag acgaaaccaa ggcgcggcgg
      421 ggcgctcttt gtctgagccg agcgcgtctg cgccgtcagg gcgggaatca tagcgtccag
      481 cagaggcagc gcgacgccgg cgccgagacc ctttaacaac gtccgtcggt gcagcgattt
      541 cctggtaacg aagttcatag cttatatccg agtttacttg ttttagcggg ttcgtttgac
      601 gcgcatctgg aattgatcgc ttgtgacgac accttcgacc agcgacgcga aggtatagtt
      661 acgcttttga gattcacggg cgatcgctct gattaagggg ccatcgtagt actgcacgtt
      721 gcgacccact gcatacatca gcagtttgct ggtcatggct tcgacaaaca gaccgggctc
      781 gcgcagcagc aactggcgca ctccgtcgac gccgtcgagg cgcgtgccat ccggcagcgt
      841 gctgacgacg tcgattggtt tgccgccgtc ttcatcgcgc caacgaccta cggcgtcgaa
      901 gttctcaagg gcaaaaccga tcgggtccat cttcgcgtgg cagccggcgc aggccgggtt
      961 agcccggtgc tgcagcatag cctcccgcat cgaaagcgac tgaccggcgc cactttcggt
     1021 tttcagtgcc ggaatattgg gcggtggggc gggcggaggc atgttcagca gattctgtaa
     1081 gacgtacttg cctcgaagca caacagaagt gcgcgttgaa tacgacgtgg gagcgaggat
     1141 acttccctga cccagaaggc ccgcgcgcgg acttcccggc ggcagctcga tcttccggaa
     1201 atggcttccc gtgacgttcg gaatgccgta gtgcttcgcc agccgttcgt tcacataggt
     1261 gtaattcgcc gtcagaagat caagcacact gcgatccgag cgtagaatgc tgtcaaggaa
     1321 aagctcggtt tcgcgctcaa acgcgggctt caggccgtcg tcgaaatcgc ggaagagata
     1381 caggtcggga tctttgctct cgacgtcatg caggaacagc cattgtgcgg cgaaattggt
     1441 gacgagcgtt tcggcgcgcg ggtctgccag catgcgggcg acttgcgcgc gaagaacatc
     1501 gggctcgcgg agcttgccgc tggcagccgc gttgagcaac tcgtcgtccg gaatgctgct
     1561 ccacagaaag aaagaaagcc gggaggcaag ctcggtatcg ctgacgggat aaggcgcccc
     1621 cgctcttgcg ctcgcgggtt ccccctctac gcgaaacagg aactgcggac tgacgagcat
     1681 gcgttcgagg gcacgttcaa ttccacaatc gaaatcgcgg tccgctcgcc ccgccgcgta
     1741 gaacttcatg gctggtgcga ggtcgccgtc cgaaacagtt cggcgatagg cgcggcgcat
     1801 gagattggaa acgatctccc tcgcgcaggc gggttcttcc gccgcagtgt ggggatggca
     1861 gctgaagata aggcggcgcg agggggtgtc gccgggacct gtcgcgttat aaggacccgt
     1921 gatggtgacg cttacgaccg acggcaaggc tccgcgcgtt cgtcccggcg gccgcagaaa
     1981 agactccgaa agagccgcgc tgcgctcgat aaaggcgaca tcgactgttc gcgggccggc
     2041 ttttaccgtc gcacggaatt gttgtttggc cggcgatgcg ggatcgtcat cgtcggctct
     2101 gcggcccgtc aggctcacca aactctggcg cgcgccgtcg atgctgagtt cgagttcgtg
     2161 cgcctcgcgc tgcagacccg agactgcgat ctgaaattcg tattcaccgt cgagcgggaa
     2221 atagctgtgt acggccattc cgcctcgcgt gccaaacggg agctgctcgt cgcgtgcttc
     2281 ctgcctctgg cgaatggggg tttggtgaag attgaccaga acgccgatgg cggggtcgcc
     2341 cgtggcgagc tggctgatct tgcgcgcggc atcgagatat cgctccatgg cgacgggtga
     2401 aacgaagagc gtgtcggcaa ggttatcgaa gccgctcgaa acgttgtcgg ccggcagaag
     2461 cgtcgtatag tccatctcct tcggcaaatt gctgagcgca agcagatcgc ggatcgaatt
     2521 tttatattcg gtgcgcgtca gccggtgcac cggcaggacg ccgccggggt tgggatgcgc
     2581 cgccgcgttt gcggctagct gccctgtgag gtattcggag agacgcttgt atacagcagc
     2641 ctcggggcga ggcgcacccg ggggaggcat agcgccggcg cggagtttgc cgaggacggc
     2701 ttcccaggtt tcagcgctgg tgtccatatg atcaagccgc gcc
//
LOCUS       DATASH010000116         3597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2587770,
            whole genome shotgun sequence.
ACCESSION   DATASH010000116 DATASH010000000
VERSION     DATASH010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2587770"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3295
                     /locus_tag="VHV99_03460"
     CDS             <1..3295
                     /locus_tag="VHV99_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX3634541.1"
                     /translation="VCVLERGREFMAGEYPRTPLEGAAQMQYNTALAQIGSPLALLEV
                     HVNEAVNAVVGCGLGGTSLINANVALEAEPRLWNDERWPAALRDDKAGRDKGYELARA
                     MLQPSAVTGDFLQLPKLQALKKSAQALGMEDRFYPPPVTVTFENRVNAAGVEQQACVG
                     CGDCNSGCNYDAKNSTHMNYLPDAVAHGAHIFTGTPVHSVLRDAATQKWIVRFQEVKL
                     GRESYDAPDLFVSADIVIVSAGTIGSTALLLRSRKEGLTVSDLLGKRFTGNGDVLAFA
                     YNTDEVINGVGWGTHKKGDIAPVGPTITGIIDHRNTADVKDGFVIEEGSLAGPVGAAL
                     VALLGGAAPLEGVEIPAPRAAGEPLGYDARVIESFLRGPYHGALNHTQTYLVMAHDDE
                     SGEISVNDTGRPRIDWPNAGKQPIFQTVEATLEKAQTPLGGKYVRNPISTRMLKNRTV
                     TVHPLGGCGMGEDAERGVVDHQGRVFSGTTGNAVHEGLYVMDGAVMPMSLGVNPLLTI
                     SALAERNCALLANARGWKIDYVAKGTAATPPPQKIGLRFTETMIGTYTPAVQGEAATG
                     PIEFTLTVESDDLAGMLSNPQHLAHTTGTLTCPALSAQPMTITDGTFNLFVVDESDVD
                     ERNMNYRMTLNSTEGKTYYLTGQKIITRTSPINLWEQTNTLYAEIRDSAQADAPPVGQ
                     ATLIITPENFLKQQRTLEVTNTPDLKTRLEWTLKFGKFFAGVLFAEYGGVAAPLQFYD
                     SKAPPRLKRVLRAPTPQVVFFDTPDGTRLRLTRYAGSPDKPLRPVLLIHGSGVSSRIY
                     STDLIATNLVEFLSTAGYDVWLVDLRVSIEMPSVLVPTNVDKVAREDIPAAVARVRQV
                     TGAPDIQVLGHCLGGLALTMSLLYGLEGVRSAVISQVSAHPVPGTLEKIKCGLHVPDI
                     MEHLGVLDLTAFTEHRSWPQNLLDEALRLYPLNHKQGCGSPICHRATFLYGLLYEHEK
                     LNETLHANLQEILGVHDVSVFKHLAAMVRAGKVVDAEGRDVYLNGADGMKGLAGMRLP
                     IGFIHGEKNETYLLKSTQLTYDMLVKHFPEQPYERHIIPGYGHIDCIFGKNAAVDVYP
                     VIARYLNAH"
BASE COUNT          736 a         1138 c         1088 g          635 t
ORIGIN      
        1 tgtgtgcgtg ctcgaacgcg gacgggaatt catggccggc gaatatccgc gcacgccgct
       61 cgaaggcgcc gcccagatgc agtacaacac ggcactcgcg caaatcggct caccgcttgc
      121 gctgctcgaa gtgcacgtga atgaagctgt gaatgcagtg gtgggctgcg ggctgggcgg
      181 tacgtcgctg atcaacgcga acgtcgcgct cgaagccgaa ccgcgcttat ggaacgacga
      241 gcgctggccc gccgcgctgc gcgacgacaa ggcgggtcgc gacaagggct acgaactggc
      301 tcgcgcgatg ctgcagccgt cggcggtaac cggtgatttc ttgcagttgc cgaaattgca
      361 ggcgctcaaa aaatccgcgc aagcgctcgg catggaggac cgtttttatc ccccacctgt
      421 caccgtcacc ttcgagaacc gtgtcaacgc tgcgggcgtc gagcagcagg cctgcgtcgg
      481 atgcggtgat tgcaactcgg gctgcaatta cgacgcgaaa aattcgacgc acatgaacta
      541 tctgcccgat gcggtcgcgc acggcgccca tatatttacg ggcacgccgg tgcattcggt
      601 gttgcgcgac gcagccacgc aaaaatggat cgtgcgtttt caggaggtga agctgggccg
      661 cgagagctac gacgcgcctg atctgttcgt gagcgccgat atcgtgatcg tatcggccgg
      721 cacgatcggc tccaccgcgc tgctgttgcg ctcgcgcaag gaggggctga ccgtttcgga
      781 tctgctcggc aaacgcttca ccggcaacgg cgacgtgctg gcctttgcgt acaacaccga
      841 tgaggtgatc aacggcgtcg gctggggtac gcacaagaag ggcgacattg cgccggtcgg
      901 gccgaccatc accggcatca tcgaccaccg caacaccgcc gacgtgaaag acggcttcgt
      961 gatcgaagag ggttcgctcg ccggaccggt cggcgccgcg ctggtcgccc tgctcggcgg
     1021 cgccgcgccg ctcgaaggcg tcgagattcc cgcaccgcgc gccgccggag aacctctcgg
     1081 ctacgacgcg cgcgtgatcg aaagcttcct gcgcggtccg tatcacggcg ccctcaacca
     1141 cacgcagact tacctggtca tggcgcacga cgacgaaagc ggcgagatca gcgtgaatga
     1201 cacaggcagg ccgcgcatag actggccaaa cgccggcaaa cagccgatct tccaaaccgt
     1261 cgaagcaacc ctcgagaagg cgcaaacgcc gctcggcgga aaatacgtgc gcaatcccat
     1321 ttcgaccaga atgctcaaga accgcaccgt caccgtgcat ccgcttggcg gctgcgggat
     1381 gggcgaggac gccgagcgcg gtgtcgtcga ccatcaaggc cgagtcttca gtggcacgac
     1441 gggcaatgcc gtacacgaag gcttgtatgt gatggatggc gccgtcatgc caatgtcgct
     1501 cggcgtcaat ccactgttga cgatttccgc gctcgcggaa cgcaattgcg cgctgctcgc
     1561 aaacgcacgg ggctggaaga tcgactacgt ggcgaagggc acggccgcca cacccccgcc
     1621 ccaaaaaatc ggcctgcgct tcaccgaaac gatgatcggc acttacaccc cggccgtgca
     1681 gggtgaagcc gccaccggtc cgatcgaatt cacgctgact gtcgaatcgg acgatctggc
     1741 cgggatgctg agcaatccac aacatctggc ccacaccacc ggcacgctga cctgccccgc
     1801 gctctccgcg caacccatga cgatcacgga cggcaccttc aacctgttcg tggtcgatga
     1861 atcggacgtg gacgagcgca acatgaatta ccgcatgacg ctcaattcaa ccgaaggcaa
     1921 aacgtactat ctgaccgggc aaaagatcat cacgcgcacg tcgccgatca atctgtggga
     1981 acagaccaac acgctctacg cggaaatccg cgactcggcg caagcggatg cgccgccagt
     2041 cggccaggca acgctgatca tcacgcccga aaatttcctc aagcagcaac gcacgcttga
     2101 agtgaccaac acgccggatc tgaaaacgcg tcttgaatgg accctgaagt tcggcaagtt
     2161 tttcgcaggc gtgctgttcg ccgaatacgg cggcgtcgcc gcgcccttgc agttctatga
     2221 ttcgaaggca ccgccacggc tgaagcgcgt gctgcgcgct ccgacgccgc aagtggtctt
     2281 cttcgacacg cccgatggaa cccggttgcg gctcacgcgc tacgccggct cgcccgacaa
     2341 accgctgcgt cccgtgctgc tgatccacgg ctcaggcgtg tcgagccgca tctattccac
     2401 cgatctgatc gcgaccaacc tggtggagtt tctcagcacg gccggctacg acgtgtggct
     2461 ggtcgatctg cgcgtcagca tcgaaatgcc gagcgtgctg gtaccgacca acgtcgacaa
     2521 ggtcgctcgc gaggatattc cggcggcggt cgccagagtg cggcaagtaa ccggcgcgcc
     2581 ggacattcag gtgctgggcc actgcctggg tgggttggcg ttgaccatgt cgctgctgta
     2641 cggcctcgag ggcgtgcgct cggcggtcat atcgcaggtg tccgcgcatc cggtgccggg
     2701 cacgctggaa aagatcaagt gcggcctgca cgtcccggac atcatggaac atctcggcgt
     2761 gctcgacctg accgccttca cggagcacag gtcgtggcct cagaatctgc tcgatgaagc
     2821 gttgcggctc tatccgctca atcacaaaca gggctgcggc agcccgattt gccatcgggc
     2881 gaccttcctt tacggcttgc tctacgaaca tgaaaaactg aacgagacgc tgcatgccaa
     2941 tctgcaggaa attctcggcg tacatgatgt ctccgtcttc aagcatctcg ccgcgatggt
     3001 gcgagcgggc aaggtcgtcg acgccgaggg ccgggacgtc tacctgaacg gcgccgacgg
     3061 catgaaaggc ctggcgggta tgcgtttgcc gatcggcttt atccacggcg aaaaaaacga
     3121 aacctatctg ctgaagagta cgcaactgac gtacgacatg ctggtcaagc actttcccga
     3181 gcagccttac gagcggcaca tcattccagg ctacgggcac attgactgca tcttcggcaa
     3241 gaacgcggcg gtggatgtgt atcccgtgat tgcgcggtat ctgaatgcgc attgacgaaa
     3301 gttgagggcg gcatcgccgt tcgtggacat ctgtgccacg aaaccgaaac gccctgccgg
     3361 cttcatgtct tacgcctgtt cctcgctgtt gcagcttgag ccggtatgta cctacacggc
     3421 tcgactcata ttccgtatgg actcggaagc atagttcgag acacaatgtg cccggcgacg
     3481 ggcggtcggc gcgcgaattc gcgctttgac attttcgcgt acacggagcg cgtgccatcc
     3541 gtccatcttg cctggcagaa ccaaaccaag cccctccttc gctagcagca aagtcaa
//
LOCUS       DATASH010000117         7222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3576224,
            whole genome shotgun sequence.
ACCESSION   DATASH010000117 DATASH010000000
VERSION     DATASH010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7222
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3576224"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(246..1196)
                     /gene="hslO"
                     /locus_tag="VHV99_03465"
     CDS             complement(246..1196)
                     /gene="hslO"
                     /locus_tag="VHV99_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEX3634542.1"
                     /translation="MSDQLQKFMFSAAPVRGEIVSLRNTWQEVLTRRDYPAPVRTILG
                     EMMAACALLSANLKFDGTLVMQIFGDGPAKMLVVQCSSDLSMRATAKLSGEAANTIAD
                     ATSMIELVNASGHGRCVITLDPRDKQPGQQPYQSIVPLSGVNGPLKSMAEVLEHYMHH
                     SEQLDTRLWLAANTERAVGMLLQKLPGDGGIVPHPGDLDADTWERVCTLGSTMSQDEL
                     LKEEPETIFRRLFWQENVQHFEPATARFECSCSREKVGAMLKMLGREEVDSVIEERGH
                     VEIHCEFCNQRYEFDPVDVTQLFVANALSQGVTPAADQRH"
     gene            complement(1261..1785)
                     /locus_tag="VHV99_03470"
     CDS             complement(1261..1785)
                     /locus_tag="VHV99_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEX3634543.1"
                     /translation="MTIYKLGEAAPTIHESVFVADSANIIGNVTLEANSSVWFGATLR
                     GDNEPITLGAGSNIQENAVLHTDPGFPLTIEPNVTIGHQVMLHGCTIKEGSLIGIQAV
                     VLNGAVIGRNCLVGAGAIVTEGKVFPDNSLILGAPAKVVRELTETDIANMQRGTAGYA
                     ERREYFKAQLVRIG"
     gene            2173..3084
                     /locus_tag="VHV99_03475"
     CDS             2173..3084
                     /locus_tag="VHV99_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX3634544.1"
                     /translation="MNSAAPSVSPAAPIPHPEKLSSGTNGGHCARTAALAALREIDPA
                     TKSAAARALYAAALDGSLACPADLELAEPADLPGRPARPELVDPRNLKRRSMQSPEGR
                     AVLLHALAHIEFNAINLALDAVWRFAGMPAAFYTDWLKVAAEEAHHFSLLVARLAEFG
                     HVYGDFPAHDGLWDMCERTRGDVLARMALVPRTLEARGLDASPPIRERLQQAGDHASA
                     AILDVILRDEIGHVLIGNRWFRHLCEEGGLDPHATYTRLADQYHAPKLRGPFNFEARR
                     DAGFDETELAALAGLDARRTVAPVSNG"
     gene            3169..4053
                     /locus_tag="VHV99_03480"
     CDS             3169..4053
                     /locus_tag="VHV99_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3634545.1"
                     /translation="MNTSRSEFVAVRGIRLHVRRWGNPDAPMLFMLHGWMDVAASFQF
                     VVDALGGDWQVIAPDMRGFGLSDWPVAERGGGNYWIQDYLADLDALLDHYAPTGEVNL
                     VGHSMGANIVCMYAGVRPERVRRVVDLEGFGLAPSHAAQAPRRLRNWLDELRDPPRLK
                     HYASLEDVAGRLIKTNPRLAPQRAQFLAQHWSKPDGEGRFMLLADPAHKLRGPTLYRL
                     DEVMAVWRKVSARVLHVEAANSPTLAQIAGEIPLDEFKARFQTFPNWREKVIEEAGHM
                     VHHDQPEQVAALIEGFCA"
     gene            4157..4987
                     /locus_tag="VHV99_03485"
     CDS             4157..4987
                     /locus_tag="VHV99_03485"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014723269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097657 - 3',5'-nucleotide bisphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-nucleoside bisphosphate phosphatase"
                     /protein_id="HEX3634546.1"
                     /translation="MNADLHCHSTVSDGQFAPAEVARRAHEGGVTLWALTDHDELGGQ
                     HEARSAAEALGMDYLSGVEISVTWASRTVHIVGLGVDPTSSILIDGLARTRNGRAARA
                     EAIGELLATLGIPDAYEGALKYVSNPDMISRTHFARFMVESGHCSSTQDVFNRYLGDG
                     KPGCISHRWSKLADAVGWIQAAGGEAVIAHPGRYAYTPVEFDAFFAEFIDLGGKAIEV
                     VTGSHTPDQYREYADVARRFGFEASRGSDFHAPGEGRVDLGTLPPLPADLKPVWERWL
                     "
     gene            5118..5753
                     /locus_tag="VHV99_03490"
     CDS             5118..5753
                     /locus_tag="VHV99_03490"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007580400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX3634547.1"
                     /translation="MSQYFRLHPDNPQPRLVQQAVQIINDGGVVALPTDSSYALACHL
                     DDKDAVERLRRIRGLDEKALLSLLVRDLSELSNFAMVDNRQYRLIKSVTPGPYVFVLQ
                     ATREVPRRLSHQSRKTIGLRVPDHAITLAILEELGQPLLGSTLIMPGETQPLNDPEEI
                     RERLEKQLDLVIDGGPCVCEPSTVIDLTGAEPVLVRPGRGSLAPFGLEETA"
     gene            5801..6463
                     /locus_tag="VHV99_03495"
     CDS             5801..6463
                     /locus_tag="VHV99_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009980725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX3634548.1"
                     /translation="MDSSLIQTIAVYALPVIFAITLHEAAHGYVARWLGDNTAYVLGR
                     VSINPMRHIDPLGTIAIPLLLYFATSGAFMFGYAKPVPVAFGNLRNPRWGSLWVALAG
                     PASNFVQALVWGLVGVGLAVTSVDEPFFTRMAAAGVGVNLVLGVLNLFPLPPLDGGRV
                     LMALLPPRQSIALSRLEPYGFFIVMALVMTGTLTRYWLSPLVTLGYGAITAILTPLVS
                     LF"
     gene            6470..>7222
                     /gene="trpS"
                     /locus_tag="VHV99_03500"
     CDS             6470..>7222
                     /gene="trpS"
                     /locus_tag="VHV99_03500"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX3634549.1"
                     /translation="MFPDRIFSGMRPTGSLHLGHYHGVLKNWVRLQSEYPCFFCVVDW
                     HALTTHYETPEVIEKNVWDVLIDWLASGIDPAQATLFIQSKVPEHAELALLLGMSTPL
                     GWLERVPTYKEQQEKLKDKDLSTYGFLGYPVLMAADILLYRGSLVPVGEDQVPHVEMT
                     REIARRFNYLYGREPGFEEKANEAAKKLGGKRAKLYHELRNAYQQEGDDEALEQARAM
                     LQDSQSLSMSDRERLFGYLEGSRKIILVEPQAL"
BASE COUNT         1236 a         2342 c         2269 g         1375 t
ORIGIN      
        1 agcaccgcgc tgcctgcgag ggcggtcagc acggtgaacg cgcgcatgca atgcttcaat
       61 gaagtacgga tgtccatttt tgatctccat tggtcggagg tctgagcaga acatgcgcag
      121 cgcgcaccgc caacgatcca ccgcaacatt caacgataaa gcgccgcctg actcctgaaa
      181 aggcgacaag cggcgcgtta ttttactgct gcaatacggg cgtagcgaac cgccatctga
      241 gcgcgtcagt gccgctggtc ggcagccggt gtcacacctt gtgagagcgc gttcgcgaca
      301 aaaagttgcg tcacgtcgac cggatcgaat tcgtaccgtt gattacagaa ttcgcaatga
      361 atctcgacgt gaccgcgttc ttcgatcacg ctgtccactt cctcgcggcc cagcatcttc
      421 agcatggcgc cgactttttc gcgcgagcaa ctgcattcga agcgcgcggt agcgggttcg
      481 aaatgctgca cgttctcctg ccagaagagg cggcggaaaa tcgtttccgg ttcttccttc
      541 agcaactcgt cttgcgacat cgtgctgcca agcgtgcaga cgcgttccca tgtgtccgca
      601 tccagatcgc ccggatgcgg gacgataccg ccgtcgcccg gcagtttttg cagcagcatg
      661 ccgaccgcgc gctcagtgtt cgccgcgagc cacaggcgcg tgtcgagctg ctccgagtga
      721 tgcatgtaat gctcgagcac ttcggccatc gatttgagcg ggccgttcac acccgacagc
      781 ggcacgatgc tttgatacgg ttgctggccg ggctgcttgt cgcgcgggtc gagcgtgatg
      841 acgcaacggc catggccgct cgcattgacc agttcgatca tggaggtggc gtcggcgatc
      901 gtattggccg cttcgccgga gagcttggcg gtggcgcgca tcgacagatc ggagctgcac
      961 tgcaccacca gcatcttggc cggcccgtcg ccgaaaatct gcatgacgag cgtgccgtcg
     1021 aacttgaggt tcgccgaaag cagcgcgcac gcggccatca tctcgcccag aatcgtccgt
     1081 acgggcgccg gatagtcccg gcgcgtcagc acttcctgcc atgtattgcg cagcgaaacg
     1141 atctcgccgc gcaccggcgc cgcgctgaac atgaattttt gcaactggtc gctcacaact
     1201 tttcctcggt cgaatggcgc agcctgatgc cgcgccgtgc gcgccgcgcg ctccggctga
     1261 ttagccgatg cgcacgagct gcgctttgaa atactcgcgg cgctcggcat agcctgccgt
     1321 gccgcgctgc atattggcga tatccgtctc ggtcagttcg cgcacgactt tggcgggtgc
     1381 gccgagaatc agcgaattgt cgggaaacac tttgccttcg gtgacgatgg cgcccgctcc
     1441 gaccagacaa ttgcggccga ttaccgcacc attcaagacc actgcctgaa ttccgatcag
     1501 cgagccttcc ttgatcgtgc agccgtgcag catgacctgg tggccgatcg tcacattcgg
     1561 ctcgatggtc agcgggaagc ccgggtcggt gtgcagcacc gcgttttcct gaatattgct
     1621 gccggcgccg agggtgatcg gctcgttgtc gccgcgaagc gtcgcgccga accagacgct
     1681 cgaatttgcc tcgagggtca cattgccgat aatgttcgct gaatccgcga cgaacacgct
     1741 ttcatggatg gttggggctg cttcaccaag cttgtaaatt gtcacgttgt ctcctcttga
     1801 tcggtcgctg cgcaatgcgc gggcgggctg gggcttcgaa aaccgggctg gcggacgagg
     1861 ctgccaggcg gcgccgccca aggcggaacg aagcgggttc aagtgggccc aaagcgggca
     1921 aagtgggctc gaagcggcgc tccaaacggg gcttttcaac gtagcttgcc agtcggggcc
     1981 gcgttgtacc tggaaacaca tcgacgactc ggactatccc gccggccccg cgcgagtggg
     2041 tcgaatcgag tattgtaaac ggttgtgtgc ttaggccgct tggcggtgcc gcgagccatc
     2101 tggcgccgcg ctcgcgctaa cgccgcacct cccgtcccgc cgatcactgt gacctttgcc
     2161 aaccggcttg ccatgaattc tgctgctccg tctgtttcgc ccgctgcgcc gataccacat
     2221 cctgagaaat tgtcgagcgg cacgaatggc ggccactgcg cgcgcactgc agcgttggct
     2281 gcattgcgcg aaatcgaccc ggccaccaag tccgccgcgg cgcgcgcgtt atacgccgcg
     2341 gcgctcgacg gcagcctcgc atgccccgcc gatctcgaac tcgccgagcc ggcggacttg
     2401 ccgggccgtc ccgcgcgccc cgaattggtc gatccgcgca acctgaagcg ccgcagcatg
     2461 cagtcccccg aggggcgagc cgtcttgctg cacgcgcttg cgcacatcga attcaatgcc
     2521 atcaatctcg cgctcgacgc cgtctggcgc tttgccggca tgcctgccgc gttctacacc
     2581 gactggctta aagtcgctgc cgaagaggct catcacttct cactgctggt cgcgcggctc
     2641 gcggaattcg gccatgtcta cggcgacttt cccgcgcacg acggcctctg ggacatgtgc
     2701 gagcgcaccc gtggcgacgt gctggcgcgc atggcgctgg tgccgcgcac cctcgaggcg
     2761 cgcggcctcg acgcctcgcc gccgatccgc gagcgcctgc aacaggcggg cgaccatgcg
     2821 tccgcggcga ttctcgacgt gatcctgcgc gatgaaatcg gccatgtgct gattggtaac
     2881 cgctggttcc gccatctgtg cgaggagggc ggcctcgatc cgcacgccac ctacacgcgc
     2941 cttgccgatc agtatcacgc accgaaatta cgtggtccgt tcaatttcga ggcgcgccgc
     3001 gacgccggtt tcgacgaaac cgaactggcc gcgctggccg gcctcgacgc gaggcgaacg
     3061 gtcgcgccgg tctcgaacgg ctgaggcttt tttccacact ccggccccac gtatcgccca
     3121 gctatctcgt tataatcgaa cgatcattct tttcccgacg cgccgctcat gaatacttcc
     3181 aggtctgaat tcgtcgccgt gcgcggcatc cgtctgcacg tgcggcgctg gggcaatccg
     3241 gatgcgccga tgctgttcat gctgcatggc tggatggatg tcgcggcgtc gttccagttc
     3301 gtcgtcgacg cattgggtgg cgactggcag gtgatcgcgc cggatatgcg cggcttcggc
     3361 ctgtcggact ggccggttgc cgagcgcggc ggcggcaatt actggattca ggactacctc
     3421 gccgatctcg acgcgctgct cgaccactat gcgccgaccg gcgaagtcaa tctggtcggc
     3481 cacagcatgg gcgcgaacat tgtttgcatg tatgcgggcg tgcggccgga gcgggtgcgg
     3541 cgcgtggtcg acctggaggg cttcggtctc gcgccgtcgc acgcggctca ggcgcccagg
     3601 cggctgcgta actggctcga cgagctgcgc gacccgccgc gattgaagca ctacgcgtcg
     3661 ctggaagacg ttgccgggcg cctgatcaag accaacccgc gcctcgctcc gcagcgcgcg
     3721 cagttcctcg cacagcactg gtccaagccg gacggggaag ggcgcttcat gctgctcgcg
     3781 gatccggcgc acaaactgcg cgggccgacg ctgtaccgcc tcgacgaagt gatggctgtg
     3841 tggcgcaagg taagcgccag ggtactgcac gtcgaagccg ccaactcccc tacgctcgcg
     3901 cagatcgccg gcgagattcc actcgacgag ttcaaggcgc gtttccagac tttcccgaac
     3961 tggcgcgaaa aagtcatcga agaagcgggg cacatggtgc atcacgacca gccggagcag
     4021 gtggcggcgc tgatcgaggg tttctgcgcg tagcgctgac caaaagctcg ggtcgccaag
     4081 gccgcctttg cgagatagcg ctgcgcccga ttactgcgtt gcagtaaaat gagcgcagac
     4141 ccttctcaag acaaagatga acgccgacct ccactgtcac tccaccgttt ccgacggcca
     4201 gttcgccccg gccgaagtcg cgcgccgcgc gcacgagggc ggcgtgacgc tgtgggcgtt
     4261 gaccgatcac gacgaactgg gcggccagca tgaagcgcgc agcgccgccg aggcgctcgg
     4321 catggactac ctgagcggcg tggagatttc ggtcacgtgg gcttcgcgca ccgtgcacat
     4381 cgtcggcctt ggggtcgatc cgacaagttc gatcctgatc gacggcctcg cgcgcacccg
     4441 caacggccgc gccgcgcgcg ccgaagcaat cggcgagctg ctggctacac tcggcattcc
     4501 cgacgcctat gagggcgcgc tcaaatacgt gtcgaatccc gacatgattt cgcgcacgca
     4561 cttcgcgcgc ttcatggtcg aaagcggcca ctgcagctcg acgcaagacg tgttcaaccg
     4621 ctatctcggc gacggcaaac ccggctgcat ctcgcatcgc tggtcgaaac tggcggacgc
     4681 agtcggctgg attcaggcgg ccggcggcga ggcggtcatc gcgcacccgg gccggtacgc
     4741 gtacacgccg gtcgaattcg acgcgttttt tgctgagttc atcgacctcg gcggcaaggc
     4801 gatcgaagtg gtgacgggta gccacacgcc ggatcaatac cgcgaatatg cggatgtcgc
     4861 gcgccgcttc ggcttcgaag cctcgcgcgg ctccgacttc catgcacccg gcgaaggccg
     4921 cgtcgacctc ggcacgctgc cgccattgcc cgccgatctc aagcccgtct gggaacgctg
     4981 gctgtgatcg cgccccgtgc cgcggcggca cgcgatgtgc gaagacggtc gtccggggcg
     5041 ggtgcttccg cctcgtcctc cgtgtgattt cagcctgact ttccgtccat cgtcacccgt
     5101 agccactgta agtcactatg tctcaatact ttcggcttca tccggataac ccgcagcccc
     5161 gcctcgtcca gcaggcggtg cagatcatca acgacggcgg cgtggtcgcc ttgccgacgg
     5221 attcgagtta cgcacttgcc tgtcatctcg atgacaagga cgccgtcgag cgtttgcggc
     5281 ggattcgtgg gctcgatgaa aaggctctgc tgtcgctgct ggtgcgcgat ctgtcggagt
     5341 tgtcgaactt cgcgatggtc gacaaccggc aataccggct gatcaagtcg gtcacgccgg
     5401 gcccttacgt tttcgtgttg caggccacca gggaagtgcc gcggcgcctg tcgcaccagt
     5461 cgcgcaaaac catcggcttg cgggtgccgg atcacgcgat cacgctggcg attctcgaag
     5521 agctcggcca gccgctgctc ggctccacgc tgatcatgcc gggagaaacc cagccgttga
     5581 acgatccgga agaaatccgc gagcggctcg agaagcaatt ggacctggtg atcgacggcg
     5641 gcccatgcgt gtgcgaaccg tccacggtga tcgatttgac cggcgccgag ccggtgttgg
     5701 tgcggccggg gcgtggttcg ctcgcgccgt tcggtctcga agagacggca tgagcgaaag
     5761 cggacagacg cacgcaaacg cgttgttaca atagcgagtt atggattctt ccctgataca
     5821 aaccattgcg gtttacgcgt tgccggtaat tttcgcgatc acgctgcacg aggccgcgca
     5881 tggctacgtc gcgcgctggc tcggcgacaa taccgcgtac gtgctcggcc gcgtttccat
     5941 caatccgatg cggcatatcg atccgctcgg cacgattgcc attcccttac tgttgtactt
     6001 cgccaccagc ggcgcgttca tgttcggtta cgcgaagccc gtgccggttg ccttcggcaa
     6061 tttgcgcaat ccgcgctggg gcagcctgtg ggtcgcgctc gccgggccgg cctccaattt
     6121 cgtgcaggcg ctggtgtggg gtctggtcgg cgtggggctc gcggtgacga gcgtcgacga
     6181 gcctttcttc acgcgcatgg cggccgccgg cgtcggcgtg aacctcgtgc tcggcgtgct
     6241 caacctgttt ccgctgcctc cgctcgacgg cggccgcgtg ctgatggcgc tgctgccgcc
     6301 gcgccagtcg atcgcgctgt cgcgcctcga accgtacggc tttttcatcg tcatggcgct
     6361 ggtcatgacg ggcacgctca cgcgctattg gttgagcccg ctcgtgacgc tcggctacgg
     6421 tgcgattacc gcaattctga ctccacttgt ttcgcttttc taaataatca tgttcccaga
     6481 ccgtatcttc tccggcatgc ggcccaccgg gtcgctgcac ctcggccact atcacggcgt
     6541 gctgaaaaac tgggtccggc tgcagtccga atacccgtgt ttcttctgcg tggtcgattg
     6601 gcacgcgctg acgacgcact acgaaacgcc tgaagtgatc gaaaagaacg tgtgggacgt
     6661 gctgatcgac tggctcgcct cgggcatcga tccggcgcag gccacgctct tcattcagag
     6721 caaggtgccc gagcacgcgg aactcgcgtt gctgctcggc atgagcacgc cgctcggctg
     6781 gctcgaacgc gtgccgacct acaaggaaca gcaggaaaag ctgaaggaca aggatctgtc
     6841 cacctacggc ttcctcggct atccggtgct gatggcggcg gacattctgc tgtaccgcgg
     6901 ctcgctcgtg ccggtcggcg aagaccaggt gccgcacgtc gaaatgacgc gtgaaatcgc
     6961 gcgccgcttc aactatctgt acggccgcga accgggcttc gaagagaagg ccaacgaagc
     7021 cgcgaagaag ctcggcggca agcgcgccaa gctttatcac gagctgcgca acgcctacca
     7081 gcaggaaggc gacgacgaag cgctcgaaca agcgcgcgcg atgctgcagg actcgcaaag
     7141 cctgtcgatg agcgatcgcg agcgcctgtt cggttatctc gaaggctcgc gcaagatcat
     7201 cctcgtcgag cctcaggcgt tg
//
LOCUS       DATASH010000118         4606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1994485,
            whole genome shotgun sequence.
ACCESSION   DATASH010000118 DATASH010000000
VERSION     DATASH010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4606
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1994485"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="VHV99_03505"
     CDS             <1..657
                     /locus_tag="VHV99_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634550.1"
                     /translation="IHTFALARPREFLLIGTGTQAANHLEAIRELFPDARVWVKGSAP
                     ARAEAFCAAHRVYGTEGTEGTEGTYGAEGTHGTRGAQGGKVRELQPLADPAANIPDSV
                     DVVIALTTSKQPVYDEGARTNRLVIGVGAFTPAMVEIGARTIAGSALFVDDEAGARHE
                     AGDFIQAGVDWTQVGGIAAVVEKTASLPAGKPIVFKSVGCAAWDLAACRVAREALSGN
                     "
     gene            860..2341
                     /gene="zwf"
                     /locus_tag="VHV99_03510"
                     /pseudo
     CDS             860..2341
                     /gene="zwf"
                     /locus_tag="VHV99_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918419.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
     gene            complement(2452..2745)
                     /locus_tag="VHV99_03515"
                     /pseudo
     CDS             complement(2452..2745)
                     /locus_tag="VHV99_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106837.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DotU family type IV/VI secretion system protein"
     gene            2836..3247
                     /gene="narI"
                     /locus_tag="VHV99_03520"
                     /pseudo
     CDS             2836..3247
                     /gene="narI"
                     /locus_tag="VHV99_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721291.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
     gene            complement(3311..4267)
                     /locus_tag="VHV99_03525"
     CDS             complement(3311..4267)
                     /locus_tag="VHV99_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3634551.1"
                     /translation="MIDRITAMRTFIRIVDTNSFTRAAESLDIPRATATTIVQNLEAL
                     LGTALLTRTTRRLSITPEGAAYYERCAQILADIDEMEASLRHATDNLTGRLRIEMPGA
                     VASAIVLPALDDFHVRYPNLDLAIGISNRTVDLVSEAIDCSIQLGELPDSNLIARQLG
                     TLEHVTCASPAYLARHGTPANLDDLRGHVAVNCTSPHNGREVDFDFEVDGEAQNVKVK
                     GFVKVSDEQAYLTCGLQGLGMIQPARIAAQPYLDSGLLREVLPQWKPVPMPVSVAYVK
                     NRRVSPRVRAFVDWLAELFEQTEHVDQDMSRVRQLLRGLHPA"
BASE COUNT          812 a         1323 c         1504 g          967 t
ORIGIN      
        1 atacacactt tcgcgctggc caggccgcgc gaattcctgc tgatcggcac cggcacgcag
       61 gcagcgaatc atctggaagc gatccgcgag ctgttccccg acgcgcgtgt gtgggtgaag
      121 ggcagtgcgc ccgcgcgggc cgaagcgttt tgcgccgcgc atcgtgtgta tggtacggag
      181 ggcacggagg gcacggaggg cacatatggc gctgagggca cgcatggcac ccgtggcgcg
      241 cagggtggca aggtgcgcga actgcaaccg ctggccgacc ctgccgcgaa cattcccgat
      301 tcagtcgacg tggtgatcgc gctgaccacc agcaagcagc ccgtctacga cgaaggggcg
      361 cgcacgaacc ggctcgtgat cggcgttggt gcgttcacgc cggccatggt cgagatcggc
      421 gcgcgcacga tcgccggcag cgcgctattc gtcgacgacg aggccggcgc gaggcatgaa
      481 gccggtgatt tcatccaggc gggcgtcgat tggacgcagg tcggcggaat tgccgcagtg
      541 gtggaaaaaa ccgcttcgtt gccagcggga aaaccgattg ttttcaaaag cgtcggttgc
      601 gcggcatggg acctggcggc ctgtcgcgtc gcgcgcgaag ccttgtcggg caactgatcg
      661 tggaccgtgc gcgtcgccag gcacggggtg gttcttcggt ccaggttggc atcttgcgac
      721 aacctgcggc acatgttgcc gtgcaaacaa tctcaaaacg ttgcaccata ggcgtttgcc
      781 agaaaaaaat gccgggccgc cggcgttttt ttgccccttg tcttttgact tgatgccttc
      841 acgacgctgc gaccgcgcta tgacgaccca acacgcttct gccactccca acctgccgct
      901 cgacatgatc atcttcggcg gcaccggcga tctgtcgttc cgcaagctgc tgcccgcgct
      961 ctatatggcg catctgcact gcaatctgcc gccggatacc cgcattctca cgatcggccg
     1021 caagccgtgg tcgcgtgagg agtacatcaa cgatttcatg gaagcgaagg ccaagccctt
     1081 catcgaaaag aaggcgttcg atgctgccgc ctgggacaaa ttcctggcgc tgttcgagta
     1141 cgtgcgcatg gacgtcgatt cggtcgagga ctaccaccgt ctgaaagaag cgtagcgcga
     1201 cggcgtgcgc cgcgtgttct atctcgcgac ctcgccggat ctgttcacgc atatttgcga
     1261 caacctttcg gcggccggat tggtcgacga gaattcgcgt gtcgtgctcg agaagcccct
     1321 gggccacgat ctggcgtccg cgcaggagat caacacggcg gtcggcaagc atttcagcga
     1381 agcgcagatc taccggatcg accactacct cggcaaggaa actgtgcaga acctgatggt
     1441 gctgcgcttt ggcaacgcga ttttcgggcc gctgtggcag gcgccgtata tcaagagcgt
     1501 gcagatcacg gtagccgaag aggtcggggt gggcagccgc gcgggcttct acgacaaaac
     1561 cggcgcgctg cgcgacatgg tgcagaacca cttgctgcag ctgctgtgca tcgtcgccat
     1621 ggagccgccg gtgtcgctcg atcccgatgc ggtgcgcgac gagaagctca aggtgctgcg
     1681 ctcgctgcgt cccatgacgc ccgaggatat cgcgcgcgat accgtgcgcg gtcagtacac
     1741 ggccggcgcg gtaaacggtg agccggtgaa gggctacctc gaggaagaca acgtgccggc
     1801 cggcagccgc gccgagacct tcgtcgcgtt gcgcgcgcat atcaacaact ggcgctgggc
     1861 caacgtgccg ttttacctgc gcaccggcaa gcgtatgcag aagaaggtat cggaaatcgt
     1921 tattgagttt tccgaattgc cgttctcgat tattccgagc ggcgggcgca actacggcaa
     1981 ccgtcttgtg atccagttgc agccggaaga gtcgatccag ttgcaaatgc tcgcgaagga
     2041 accgggcagc ggcatgcaca tgctgccggt gaatttgaac ctggacttgc agcaggcctt
     2101 caccgagcgg cgcgcggaag cgtatgagcg tttgttgatc gacgtgattc gcggacgcct
     2161 cacgcacttc atgcgccgcg acgaactcga agccgcatgg gcatgggccg aaccgattct
     2221 cgacggctgg gccaagtcgg gcgacaagcc gcgtgcctat acggcgggca cgtttggacc
     2281 ggctgcgtcg acggcgctga tggcgcgtga gaacgcggtg tgggcggaag aatcgcagta
     2341 agtggagggt caagcgtcgg cggtgcgtcg acgcttgaag aaagcgttgc attgaaaaag
     2401 gcggcccgtt ttcgcggacc gcctttttcg ttatgtgctt gccgttccga tctacggcag
     2461 cgagatcgtc aacgtggccg attgcggtct gagtttgacc acttgcgaat acggtgcaag
     2521 cgtgcgcaac gtctgatcgc gcaaacaggt gttcaggccg agataagcga gcaatgccac
     2581 cagcgcgaat accgcaccga tcgaccacaa cggcacctcg cgcttgagcc ggtgcgcaac
     2641 ctgacccggc agcggccaat gcggcgcgaa cggtgcgcgc ttgcccttca tatgcgcgat
     2701 ttcgccgccg attcgcgcgg tcagatacgc gagcctcgcc ggtcccgctc ttgtcgccac
     2761 gttgcttgcg ttggcgttgg cgtcgatcgg tctggcactc tggattacct gacaactcga
     2821 cggtggcgca gctgcatgac cctgctgctg atccgccggc tcggtgaccc gcgcatccgt
     2881 gcgcattcga agaccagcga tatcgtgttg cttgtgttgt tgtgggcgta actggtgttg
     2941 ggtctcagca cgatcccgat gttggcgcag catctggacg gcagcatgat gatgaaacgc
     3001 cgattgggca cagcatatcg tcacattcca gggcggcgct gcggcgctca tccctgaagc
     3061 gagctgggtg ttcaaagctc acatgttcct tgggatgaca ctgtttctgg tgttcccgtt
     3121 cacgcgcctc gtgcatatct ggagcggttt tgcctcgctc gcgtatctgc tgcggccgtg
     3181 gcaggtagtg cgccaccgac atttgaacgt gcccgctggg cagaatacgc cgcggcggcg
     3241 tttgtaaatt aaacggacca tcactgccga actcacgcct ctgggtgctg cggacctgca
     3301 tgaacccgcc tcaggcagga tgcaggccgc gcaacagttg acggactcgc gacatgtcct
     3361 gatcgacgtg ttcggtctgt tcgaacaatt ccgcgagcca gtcgacgaag gcccgcacgc
     3421 gtggcgacac ccggcggttc ttcacatacg cgaccgagac cggcatcggc acgggtttcc
     3481 attgcggcag tacttcgcgc aggagtccgg aatcgaggta gggctgagcg gcgatccgtg
     3541 cgggttgaat catgcccagg ccttgcaatc cgcaagtgag ataggcctgc tcgtcgctga
     3601 ccttgacgaa gccttttacc ttgacgtttt gcgcttcacc gtccacttcg aagtcgaagt
     3661 cgacttcacg gccgttatgc ggcgacgtgc aattgactgc gacgtgtccg cgtagatcgt
     3721 cgagattggc gggcgtgccg tggcgggcca ggtaggccgg gcttgcgcat gtcacgtgct
     3781 cgagggtgcc caattgccgc gcgatcagat tcgagtctgg caattcgcct aactggatac
     3841 tgcagtcgat ggcttccgag accaggtcga ccgtgcggtt gctgatgccg atggccagat
     3901 cgaggttcgg gtagcggacg tggaagtcgt ccagtgccgg caagacgatc gcgctcgcta
     3961 ctgcgccggg catttcgatg cgcagccggc cggtcaggtt atctgttgcg tgacggaggc
     4021 tggcttccat ttcgtcgatg tcggcgagga tttgcgcaca gcgctcgtag taggccgcgc
     4081 cttctggggt aatgctcagg cgccgcgttg tgcgggttag gagggcggtg cctagcaggg
     4141 cttccaggtt ctggacgatg gttgtcgcgg tcgcccgggg gatgtcgagg gattccgctg
     4201 cgcgggtgaa gctgttggtg tccacgattc ggatgaatgt ccgcattgca gttattctgt
     4261 cgatcatggg tcagactcca attagtgtta aggggtgttc ttttttgcct ttggcggcgg
     4321 ctggttgtgt gcctgtggtg gttggttttt gtttgtttgt tttgcctgcg tggtgcttgt
     4381 ttgtctgtgc tttttatggc gttggccttt ccttgacctg ggtttttggc ctttccttga
     4441 attgttatcg gtctattagc gtcgcccctg tgcggggcgg gcacctactt ttctttgcct
     4501 gccgcgtaac tattcagcgg cagacaaagg cgaaacgacg attccggcaa agctggaggc
     4561 cagtgcatca atcagcgaat ggttctgctt acgcaaggtg gcaagg
//
LOCUS       DATASH010000119         3771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1994583,
            whole genome shotgun sequence.
ACCESSION   DATASH010000119 DATASH010000000
VERSION     DATASH010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1994583"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1677)
                     /gene="glyS"
                     /locus_tag="VHV99_03530"
     CDS             complement(<1..1677)
                     /gene="glyS"
                     /locus_tag="VHV99_03530"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEX3634552.1"
                     /translation="MTQPHQATLLVELLTEELPPKALARLGDAFAEGIAQRLAARDLI
                     EGELSFERYATPRRLAVTIRNVRSVAPEKHVREKVLPVSVALDKDGQPTAPLAKKLAA
                     LGFPDFSVSDLERAQDGKAEAFFLRYAAPGATLAEGLQAALDETLARLPIPKVMTYQR
                     PDGTNVQFVRPVHRLTTLHGEQVVPVSALGVDADDTTLGHRFLSEGFVQIQHADAYAD
                     TLMHKGRVVANFADRKETIRTHLLAQAEGDQVVMPESLLDEVTSLVEWPVVYACRFED
                     EFLQVPQECLILTMQTNQKYFALTDANGKLRSRFLIVSNIETRTPGDIVEGNERVVRP
                     CLADAKFFFEQDKKKPLADRVPLLANVVYHNKLGSALQRVERLEALAGAIASLIEAGR
                     QGHQAAGEVHALLAGDVALARRAARLAKADLITDMVGEFPELQGTMGTYYARHDGEPE
                     EVALACSEHYLPRFSGDTLPATATGTIVALADKLETLVGIWGIGLQPTGEKDPFALRR
                     HALGVLRILVEKQLPVDLIELLRAAYATFAAVPGVADSTQAIYDFSMDRLR"
     gene            complement(1698..2705)
                     /gene="glyQ"
                     /locus_tag="VHV99_03535"
     CDS             complement(1698..2705)
                     /gene="glyQ"
                     /locus_tag="VHV99_03535"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014896110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEX3634553.1"
                     /translation="MLTFQQIILTLQSYWDKQGCALLQPIDMEVGAGTSHVHTFLRAI
                     GPEPWRAAYVQPSRRPKDGRYGENPNRLQHYYQYQVVLKPAPENILDLYLGSLEALGF
                     DLKQNDVRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLDCKPVLGEITY
                     GLERLAMYLQKVENVYDLVWTEWEEQGPNGPELRRLTYGDVYHQNEVEQSTYNFEHAN
                     VDLLFTFFNSYEAEARRMIEAQIALPAYELVLKAGHTFNLLDARGAISVTERAAYIGR
                     IRALSRLVAQAYYDSREKLGFPMLGNPVHGVPGLTTDEQDAAMPAWVPPLKIERKIDP
                     N"
     gene            3383..>3771
                     /locus_tag="VHV99_03540"
     CDS             3383..>3771
                     /locus_tag="VHV99_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634554.1"
                     /translation="MTKTTKKTHPATRPSVDALQYEKLALSAHNLCDRQLDQLRRLAI
                     LAASICRSPSMTREERQSQKALLELLVETAEGYQREIECDRELYQVIALDAKGVPHSR
                     ITANVAAKLLSEAAQVAGQGWIDTEQS"
BASE COUNT          690 a         1110 c         1204 g          767 t
ORIGIN      
        1 gcgcagacgg tccatgctga agtcgtagat cgcttgcgtg gaatcggcga cgcccggcac
       61 ggcggcgaag gtcgcgtaag ccgcgcgcag cagttcgatc aggtcgaccg gcaattgctt
      121 ctcgaccagg atccgcagca caccaagcgc atgacggcgc agcgcgaacg ggtctttctc
      181 gccggttggt tgcaggccga tgccccagat gccgaccagc gtttcgagct tgtcggcgag
      241 cgcgacgatg gtgccggttg ccgtggcggg cagtgtgtcg ccggagaaac gcggcagata
      301 gtgttccgag cacgcgagcg cgacctcttc cggctcgccg tcgtggcgcg cgtagtacgt
      361 gcccatcgtg ccttgcagct cggggaattc gccgaccatg tcggtgatca gatcggcttt
      421 cgccagacgc gcggcgcgcc tggccagcgc cacgtccccg gcaagcagcg cgtgaacctc
      481 gccggccgcc tgatgaccct gccgacccgc ttcgatcagc gaagcaatcg cgccggccag
      541 tgcttccagc cgctcgacgc gttgcagcgc ggaacccagc ttgttgtgat acacgacgtt
      601 cgcgagcagc ggcacacggt cggccagcgg cttcttcttg tcctgctcga agaagaattt
      661 cgcatccgcc agacacgggc gcaccacgcg ctcgttacct tcgacgatat cgcccggcgt
      721 tcgggtttcg atgttcgaca cgatcaggaa gcgcgagcgc agcttgccgt tcgcatccgt
      781 caacgcgaag tacttctggt tcgtctgcat cgtcaggatc aggcattcct gcggcacttg
      841 caggaattcg tcctcgaaac ggcaggcgta gaccaccggc cattcgacca gcgacgtcac
      901 ttcgtccagc agcgattccg gcatgacaac ctgatcgcct tcggcttgcg cgagcagatg
      961 cgtgcggatg gtttccttgc ggtcggcgaa attcgcgacc acgcggccct tgtgcatcag
     1021 cgtgtccgcg tacgcatccg cgtgctggat ctgcacgaag ccttcggaca ggaagcgatg
     1081 gccgagcgtg gtgtcgtcgg catcgacgcc tagcgcgctg accggtacga cctgttcgcc
     1141 atgcaacgta gtcagacgat gcaccggacg cacgaactgt acattggtgc cgtccgggcg
     1201 ctgataggtc atcaccttcg ggatcggcag cctggcgagc gtttcgtcga gcgcggcttg
     1261 caggccttcg gcgagcgtgg cgcccggcgc ggcgtagcgc aggaagaacg cttcggcctt
     1321 accgtcttgc gcgcgttcca gatcgctcac cgagaagtcg gggaagccga gcgcggccag
     1381 tttcttggcg agcggcgcgg tgggctgacc gtctttatcc agcgcgacgg aaaccggcag
     1441 cactttttcg cgcacgtgtt tttccggcgc aaccgaacgc acgttcctga tcgtgacggc
     1501 gaggcggcgc ggcgttgcgt aacgttcgaa cgacagttcg ccttcgatca gatcgcgcgc
     1561 cgcgaggcgt tgcgcaatgc cttcggcgaa ggcgtcgccg agacgcgcga gcgctttcgg
     1621 cggcagttct tcggtcagca gctcgacgag cagggtagct tgatggggtt gagtcatttc
     1681 ctggtgttct cgtctcttca gttcggatcg atcttgcgtt cgattttgag cggcggcacc
     1741 cacgcgggca ttgcggcatc ttgctcgtcg gtcgtgaggc cgggcacgcc gtgcaccgga
     1801 ttgccgagca tcgggaaacc gagcttttcg cgcgagtcgt agtaagcctg cgcgaccaga
     1861 cgcgacagtg cgcgaatccg gccgatatac gccgcacgtt ccgtgacgga gatcgcaccg
     1921 cgtgcgtcga gcaggttgaa cgtgtggccg gccttcagca ccagttcgta agcgggcagc
     1981 gcgatctgcg cttcgatcat gcgcctcgct tccgcttcgt agctgttgaa gaacgtgaac
     2041 agcagatcga cgttcgcatg ctcgaagttg taggtggatt gttcgacttc gttctggtgg
     2101 tacacgtcgc cgtaggtcag gcgacggagt tccggaccgt tcgggccttg ctcttcccac
     2161 tcggtccaga ccaggtcgta gacgttctcg accttctgca gatacatcgc gagacgttcg
     2221 agaccgtagg tgatttcgcc cagcaccggc ttgcaatcca ggccgccgac ctgctggaag
     2281 taggtgaact gggtgacttc catgccgttc agccacactt cccagccgag gccccaggcg
     2341 ccgagcgtgg ggttttccca gtcgtcttcg acaaagcgca cgtcgttctg cttcaggtcg
     2401 aagccgaggg cttccaacga gccgaggtac agatcgagga tgttttccgg cgccggcttc
     2461 agcaccacct ggtactggta gtaatgctgc aggcggttcg ggttctcgcc gtagcggccg
     2521 tctttcgggc ggcgcgacgg ctgcacatac gcggcgcgcc acggttccgg accgattgcg
     2581 cgcaggaagg tgtggacgtg ggacgtgccc gcgccgactt ccatgtcgat cggctggagc
     2641 aacgcgcaac cctgtttgtc ccagtaggac tgcagtgtca ggatgatttg ctgaaacgtg
     2701 agcatgaagt gcctttcggg catggggccg cgccgtaagc gccgaagcgt gcaggctggc
     2761 cgtggagcga agtgctaaac cgtagatttt aacggaaagg gaggggggtg tccgttttgc
     2821 ggggtctgac gggaactgaa gggttccttg ggcgacaaaa ggggtgtttc tggcaggttt
     2881 catagttctt tcgactcgcg atgaaaccgt tggccgatgt cgtcagcgca cgcacgcatg
     2941 caggcgcgtc gtgtcgattt ggtcgtcttt tgtttcaact gggcacggca gtgggttgac
     3001 catgcaccga tcgtagtcga gtgccggcgt ccgttgcacg ataaccagta aggagcgcct
     3061 gcagggccga atgtaaagat tagttataat ttgcgcgttt agcttggcca gttgatcctc
     3121 tcaaaaaata gttgatttac attgtttctg ccgttagaca aaacggcgcg ggtgtgagag
     3181 ctcgtagcga tgtaaccgca ctacccgcga gagtgggtag tgctccgcca tgcgcgcctt
     3241 tctatgggcg ggccgtggtg agggagcctt gcggctcgcc ggttatggtt tcatcgcccg
     3301 gtctctcaaa ctcgctacgt gcccgctcac cccatctgtt ggcgagtgtc gggcgattca
     3361 ggaacaaacc ggaaatcgca ctatgaccaa aaccactaaa aagacccacc ccgcaacacg
     3421 tccttccgtt gatgctctcc agtacgaaaa actggcacta tcagcgcaca acttgtgcga
     3481 ccgccagttg gaccaactca ggagactggc tattcttgca gcctcaatat gcaggagtcc
     3541 ttccatgaca agggaggagc gacaaagcca gaaagcgcta ttggaactgc tggttgagac
     3601 tgccgaggga tatcaaagag agattgaatg cgaccgcgaa ctgtaccagg taatcgccct
     3661 tgacgctaag ggggttcctc acagtcgcat cacggccaac gttgcggcga aactgctttc
     3721 cgaggctgca caagtggcag ggcaaggttg gattgacacg gagcaaagta t
//
LOCUS       DATASH010000120         3779 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2192649,
            whole genome shotgun sequence.
ACCESSION   DATASH010000120 DATASH010000000
VERSION     DATASH010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2192649"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..126)
                     /locus_tag="VHV99_03545"
                     /pseudo
     CDS             complement(1..126)
                     /locus_tag="VHV99_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031313474.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            195..422
                     /locus_tag="VHV99_03550"
     CDS             195..422
                     /locus_tag="VHV99_03550"
                     /inference="COORDINATES: protein motif:HMM:NF016513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEX3634555.1"
                     /translation="MKNYLFEVIAAVTLALGVAMNLELRPPGSAVTTVLFIALQPQSG
                     MVFAKSFYRICGTLMGLVVMPALLGLLWGFD"
     gene            496..2700
                     /locus_tag="VHV99_03555"
     CDS             496..2700
                     /locus_tag="VHV99_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007585431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEX3634556.1"
                     /translation="MPTSPSSVPDPTPRLAAFYTAAVDWARTDGRTWIYLFKALAAAL
                     LALGVAMKLELPQPGSAMTTVFIVMQPQSGMVFAKSFYRICGTLVGLVVMLLLLGLFA
                     QQPELFIVSTAIWIGVCTAGAARYRNFRSYGFVLAGYTAAVIGFPASQHPDGAFLSAL
                     ARVTVVMVGIISAGTVSALVFPQYAGEKMRTAVCTRFSSFVEFLSTSLSGRPDRSHIE
                     AANARFVDDIVGFEAIRSVAVFDSPDARMRSGRLSRLNSEFMAASTRFYALHQLINRL
                     HDAGSSVTIDALEPYFKEIAPLLNKSGEPVVSAADAAHAAVQLEGFKVELPRRVRATR
                     AELEVRPDVRLLDFDTAAELLYRFVDDLHAYTTTYASLAVDTHERERWVERYEPKTNA
                     IAAGVAGVRAALVIMVLGAFWIATAWPSGSMLVLTAATYCALVSSSPYPTRFAFQMAG
                     GTLLSSLAGLIAMFVIYPHINGFPLLCAALTPFLLLGVYMTTKPSIAGYGIGYCIYFC
                     FLAGPDNLIHYDPMSFMNNALALVLALLVSSIAFAVLLPPSTPWLRNRLLVDLRRQVV
                     AACHARLTRLPSRFESGTRDLMFQISALAESEPEVKRDTLRWLFAVLEVGNAMIHLRS
                     KLATLPADACFAETMPWRVTIRIMRDAVAVLFHRPRADRLDAALSATDDAIAVVQQTL
                     ATFTPPREERHRLRRILNDLHFIRSALLDPQSPLAALAGARADAQEGVHHAT"
     gene            2690..2890
                     /locus_tag="VHV99_03560"
     CDS             2690..2890
                     /locus_tag="VHV99_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HEX3634557.1"
                     /translation="MPREVAVLDAYVPAIVLLFIAGAAIMWLLDGILAYTGLYRVVWH
                     PSLFRASLLACICGMLGLAVYR"
     gene            2904..3764
                     /locus_tag="VHV99_03565"
     CDS             2904..3764
                     /locus_tag="VHV99_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007585428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEX3634558.1"
                     /translation="MTIRNIFGFVTTAVTFVVAVLIGRVLWVRYMDDPWTRDGRVRAE
                     VVDIAPDVSGAVVELPVRDNQSVKKGDLLMQIDPSHYQIAVEQAQATVAARKAELQMR
                     RDDAQRRADMDSLVVSKENRDNATLSASTAEAQYQQAQAELDAAKLNLARTRVVSPVD
                     GYVTNLSVFRGDYATVGAAKLAIVDSHSFWVYGYFEETKLPRVKVGDRAEMRLMSGGV
                     LQGHVESISRGIYDRDNPESRELLADVDPTFNWVRLAQRVPVRIKIERIPDSVVLSAG
                     TTCTVVIATH"
BASE COUNT          628 a         1161 c         1215 g          775 t
ORIGIN      
        1 cggcgggttc aggtacagcc cgatgacgtc ggccgccttc gtttcaaagt cgggatcgtt
       61 ggacaccatg tgggtgtcga gtcgatgagg acggatatcg tgcttgcacc agatgcgttg
      121 caccgtcatg aacggcacgc ccaactgcgc ggcgagctta cgagtgctcc agtgggtacg
      181 caccgtacgg tcgcatgaaa aactatctgt tcgaggtgat cgccgccgtg acgctcgcgc
      241 tcggcgtcgc catgaacctc gaattgcggc caccgggcag cgcggtgacc actgtgttgt
      301 tcatagcgtt gcagccgcaa agcgggatgg tgttcgcgaa aagcttctac cggatctgcg
      361 gcacgctgat gggtctcgtt gtgatgccgg cgctgctcgg gctgttgtgg ggcttcgatt
      421 gaccatactc gcagcgcttg cagtcaccaa cccggccgcc agcgcagcgg ctgctcagta
      481 aagacagaat taaccatgcc cacctcccct tcttccgttc cggatccgac gccgcggctt
      541 gcggcgttct acacggctgc tgtcgactgg gcgcgcaccg acggccgaac atggatctat
      601 ctgttcaagg cgctcgcagc cgcgctactc gctctcggcg tcgcgatgaa gctcgagctg
      661 ccgcagccgg gcagcgcgat gaccaccgtg tttatcgtga tgcaaccgca aagtgggatg
      721 gtgttcgcga agagttttta ccggatctgt ggcacgctag tcggtctcgt cgtgatgctg
      781 ttgctgcttg ggctgttcgc acagcagccc gagctcttca tcgtctcgac ggcaatctgg
      841 atcggcgtct gtaccgcggg cgccgcacgc tatcgcaact tccgctcgta cggcttcgtg
      901 ctggcaggct atacggccgc ggtgatcggc ttccccgcat cgcagcatcc ggacggcgcg
      961 tttctgtctg cgctcgcgcg cgtgaccgtc gtgatggtcg gcatcatcag cgcgggtacg
     1021 gtcagcgcgc tggtgttccc gcagtatgct ggcgaaaaga tgcgcaccgc ggtgtgcaca
     1081 cgctttagtt cgttcgtcga attcctgtca acgtcgctgt cgggacggcc cgatcgctca
     1141 catatcgaag cggccaacgc gcgttttgtc gacgatatcg tcggtttcga ggccatccgt
     1201 agcgtggccg tgttcgacag ccccgacgcg cggatgagga gcggccggct gtcgcgtctg
     1261 aacagcgagt tcatggccgc gtcgacgcgc ttttacgcgc tgcatcagct aatcaaccgg
     1321 ctgcacgacg cgggctcgtc ggtgacgatc gatgccctcg agccgtattt caaagaaatc
     1381 gcgcctctgc tcaacaagtc gggcgaaccg gtggtgagcg ccgccgacgc agcacacgcg
     1441 gccgtccagc tcgaagggtt caaggtcgaa ttgccgaggc gcgtgcgcgc gacgcgcgct
     1501 gaactcgagg tgcgtcccga cgtgcggttg ctcgacttcg ataccgcggc cgaactgctc
     1561 taccgcttcg tcgacgacct gcacgcgtac acgacgacct atgcatcgct cgctgtcgat
     1621 acgcacgagc gcgaacgctg ggtcgaacgc tacgagccga aaacgaatgc gattgccgcg
     1681 ggcgtcgccg gtgtgcgcgc cgcgctcgtg atcatggtgc tgggcgcatt ttggatcgcg
     1741 accgcgtggc cgagcggctc gatgctcgtc ctaacggcgg cgacctactg tgccttggta
     1801 tcgtcgtcgc cgtatccgac gcgtttcgca ttccagatgg ccggcgggac actgctgtcg
     1861 tctctggcgg gactcatcgc gatgtttgtc atttacccgc acatcaacgg ctttccgctg
     1921 ctgtgcgcgg cgctcacgcc gttcctgctg ctcggcgtct acatgacgac gaagccatcg
     1981 atcgcgggtt atggcatcgg gtactgcatc tacttctgtt tcctcgccgg cccggacaac
     2041 ctgatccact atgacccgat gagcttcatg aacaacgcgc tggcgctggt gttggcgctg
     2101 ctcgtgtcat cgatcgcgtt tgccgtgctg ctgccgccat cgacgccgtg gctgcgcaac
     2161 cggttgctcg ttgatctgcg ccgtcaggtc gtggccgcct gtcacgcacg tctgacgcga
     2221 ctgccttcgc gcttcgagag cggcacgcgc gatctgatgt tccagatcag cgcgcttgcc
     2281 gaaagcgagc ctgaggtcaa gcgcgacact ttgcgctggc tcttcgccgt actcgaagtc
     2341 ggcaacgcga tgatccatct gcgcagcaag ctagcaacgt tgccggccga cgcgtgcttt
     2401 gcggagacca tgccgtggcg cgtaacgatc cgcatcatgc gcgatgctgt cgcggtgctt
     2461 ttccatcggc cgcgcgcgga ccggctggac gccgcgctgt ctgcgaccga cgatgcaatc
     2521 gctgtggtcc agcagacgct cgcgacattc acgccgccgc gtgaggagcg tcaccggttg
     2581 cgacgcatcc tgaatgatct gcatttcatt cgaagcgcgc tgctcgatcc tcaatcgccg
     2641 ctggcggcgc tggccggcgc acgcgccgac gcacaagaag gagttcacca tgccacgtga
     2701 ggttgccgtc cttgatgcgt acgtgccggc cattgtgctg ctgttcattg cgggtgccgc
     2761 gattatgtgg ttgctcgacg gcatcctcgc ctatacgggg ctgtatcgcg tcgtctggca
     2821 tccctccctt ttccgggcta gcctgctcgc gtgtatctgc ggcatgctcg ggcttgccgt
     2881 ttatcgttga ccacagtcga aacatgacca tccgaaacat ttttggcttc gtcacgaccg
     2941 cggtgacctt cgtcgtcgcg gtgctgatag gccgggttct atgggttcgc tacatggacg
     3001 atccgtggac ccgcgacgga cgggtgcgcg cggaagtcgt cgacatcgcg ccggacgtct
     3061 cgggcgcggt cgtcgagtta ccggtgcgcg acaaccagtc cgtgaagaaa ggcgacctgc
     3121 tgatgcagat cgacccgtcg cactaccaga tcgccgtcga gcaggcgcag gcgacggtgg
     3181 cagcgcgaaa ggccgagttg cagatgcggc gggacgatgc gcagcggcgc gcggacatgg
     3241 acagcctcgt ggtgtcgaag gaaaaccgcg ataacgcgac gctttcggcg agcaccgccg
     3301 aagcgcagta tcaacaggcg caggctgaac tcgatgcggc gaaactcaac ctcgcgcgca
     3361 cgcgggtggt gtcgccggtg gacggctatg tgaccaatct gagtgtattt cgtggcgact
     3421 atgcgacggt gggcgccgcg aagctggcga tcgtcgatag tcactcattc tgggtgtacg
     3481 ggtatttcga ggaaaccaag ctgccgcgag tcaaggttgg cgacagggcc gagatgcgcc
     3541 tgatgagtgg cggtgtcctg caggggcacg tcgaaagtat ttcccgaggt atctacgatc
     3601 gtgacaaccc ggaaagccgc gaattgctgg ccgatgtgga tcctacattc aactgggtgc
     3661 ggttggcgca acgtgtgcca gtgcgcatta agatcgaaag gattcctgat agcgtggtgt
     3721 tgtcggccgg cacgacgtgc acggtggtga tcgccaccca ctgatcctag caggacgta
//
LOCUS       DATASH010000121        17801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1796593,
            whole genome shotgun sequence.
ACCESSION   DATASH010000121 DATASH010000000
VERSION     DATASH010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 17801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17801
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1796593"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..965
                     /locus_tag="VHV99_03570"
     CDS             78..965
                     /locus_tag="VHV99_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006405432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX3634559.1"
                     /translation="MKAKDLFLAILVTAIWGANFSVIKLGLTSVDPFILAGLRFTLCA
                     VPAVFFVRRPNVSIGYLASYGLLFGVGLWGVVNLGIQAGLSAGIASLVLQFSAFFTIL
                     LGSVVFKESISKYQYIGIVVALFGLASIITITDGSATLLGVVLVIVGALSWSSANIII
                     KKSGTKDVLAFLIWSSLFSPIPLFAMAFLQHGAAGYVATFSRFNWTAIFSILFQVYPT
                     TLFGYGIWNMLLKKYPVSAVAPLSLLVPIFGMFGSMLIFGEHIGLSKVLATGFIVLGL
                     AIGLYGRHIGGMIRARVRA"
     gene            1007..2218
                     /locus_tag="VHV99_03575"
     CDS             1007..2218
                     /locus_tag="VHV99_03575"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3634560.1"
                     /translation="MSMFLVTTFVYRFANGLLFLAVAWNLVRSTGGGAMPLALSAIAG
                     FLPAVLVAPFARRLLAHRDMRRMTIHGVAGLFAAALAFAFVQRWYVGILVINFFVLAI
                     FFLLEGAWDGLLASIAKTLPVVEGDKLNSRQSAATQAGLMLGGLPIGILTREGGAAMP
                     FIAAAMLYLVTILCFMLLPLRRCAQSPGSPIESVDQQESEISHSTGPIMWSTLATLAL
                     VWPCLALVNMVVPLVANSQGNGTVEHAAILDAAIGLGMGFIGIAYERLISLRRRAYNL
                     LIVGMAFLIPLPFVVLLLFPYALFPLAACFFISGIGFGMFRISVRKQLITTQPAYRVG
                     QVVASCNGYGVPVLALAALFYAQTWRVGPVIPLAVFAVFGALATLSTKGGGSLGRAQS
                     SATPSCRTEVT"
     gene            complement(2211..3071)
                     /gene="fdhD"
                     /locus_tag="VHV99_03580"
     CDS             complement(2211..3071)
                     /gene="fdhD"
                     /locus_tag="VHV99_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEX3634561.1"
                     /translation="MNELDTAGQPGTVERHVLRHRGAAVESVTDHVGQEWPVALVFNG
                     ISHAVMMCTPRDLEAFAVGFAISEGIVERGSDIQDIEVELHDDDELPHAEVQLKVVQQ
                     AFVALKEKRRALAGRTGCGVCGIESIDLLDLEPERVPDTGFLQRLAPDAIARAARELP
                     EHQALTRLTGGLHAAAWCDAAGAIRYAFEDVGRHNALDKLIGQLVLDRADTKEGFVFL
                     SSRASYELVRKAARVDVPMVATISAPSSLAIAIARKAGVRLVSFCRETSYVDYDTLQS
                     TQPGPGLVPK"
     gene            3228..3704
                     /locus_tag="VHV99_03585"
     CDS             3228..3704
                     /locus_tag="VHV99_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase associated protein"
                     /protein_id="HEX3634562.1"
                     /translation="MGLNEAPLLFNFEVASSENFTYIPMSVRFNLDRFGLRITLAQWQ
                     LLPLEDRKLLARFPVEEDAEIEPNFDHALFEMLRTHANVEPEWFTPEEAPAWRRTDSV
                     PDGVAHQAGLAGLPTPDVAQWAELEPFKRYVLAKLSRKPDGNHDFVPAMKEFGAAG"
     gene            4084..7194
                     /locus_tag="VHV99_03590"
     CDS             4084..7194
                     /locus_tag="VHV99_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX3634563.1"
                     /translation="MTLFLSKRLLINLAVVAAAIGATACVAYTQISGQRDADARMLRS
                     TSVRQNLDAYHTALDGGLAALGHFEATGQAVPVNEVAAMAPVLAGLERDLRNEIAGEP
                     AMLGTLARLSADSHALQHDIADALLKSASAAPDESRAWAASTYTQLGLGLGRVEAGLA
                     ALRQQEDQALQASVAASTSETQRAMFLLIVTMLGGATLLLFTFGARESSAREKLRTVG
                     ALSRNDERFRGLFDDHPVPMYIFDSETLRFLAVNAAAIQQYGYSESEFFGMTIRAIRP
                     DSEISRLESHLQRSDVLQHGRTMAGIWHHRRKDGSMISADISYHSLSFMGRAAFFVLA
                     DDVTEQINAEAEAQRSNQMLETVIDNIPQRIFWKDQESRYLGCNMAFARDAGLSYSEQ
                     VVGKSDSEMPWRAYAELLNEHDREVVTTGVPKMNFEVDVLIDGVHRTMVTSKLPFTDS
                     DGRVIGVLGSYTDITERKRSDLALRLQSRALDASVNAILITAPSPAGNLIEYVNPAFM
                     RITGYDPAEVIGHDCRVLQRDDRDQEGVALIRQALSANREVSAVLRNYRKDGALFWNQ
                     LFIAPVPNAEGMITHHIGVINDVTDLIRYQEQLEYQANYDSLTRLPNRNLLRDRLQHA
                     LIVAQRHHKGVAVVFIDLDGFKNVNDTLGHSVGDRLLSVVAERLARCSRTSDTVARHG
                     GDEFVIVMTDTVDEQSLIAWMERVRASISEPVWLDGTELYVGCSMGASLFPQDGEDAE
                     TLMKKADLAMYRAKDMGRNTFQFYQPEMNASVGARLNLELRLRRALRDNEFLLYYQPQ
                     VDIGSGQIVGTEALVRWRDPEAGLVLPSLFIPVAEESGLIGPLSEWVLREACRQNKAW
                     QDEGLPPARVSVNLSARVFQQRDIAKLVKQVLAETGLEPQYLELELTESTIMRNAEEA
                     VSMLNELHALGIGLAIDDFGTGYSSLGYLKRFPVDRLKIDRSFVSDIGVSGDDETITS
                     AIIALAHSLKLQVIAEGVETLAQLDFLKERACDEMQGFYFARPLSTDAISELFQGGVD
                     REVATV"
     gene            7509..7841
                     /locus_tag="VHV99_03595"
     CDS             7509..7841
                     /locus_tag="VHV99_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4148 domain-containing protein"
                     /protein_id="HEX3634564.1"
                     /translation="MTSSPARTWAVLLASVVLSVASVAPAFAQSGGGGGPAGDDADAA
                     KPVPASKAATTKAERSAAAKQRRAKKNAELKQLESNGYNPSRNNPDYPTDLQNAQKKA
                     GIGAAASQ"
     gene            complement(8064..8459)
                     /locus_tag="VHV99_03600"
     CDS             complement(8064..8459)
                     /locus_tag="VHV99_03600"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX3634565.1"
                     /translation="MTDTLQLPQKPCALRVMPQPSDANVHGDVFGGWIMAQVDIAGSI
                     PASRRANGRVATIAVNSFVFKQPVFVGDLLSFYADIVKTGNTSVTVAVEVYAQRMSLT
                     EDLVKVTEATLTYVATDSDRRPRALPVLD"
     gene            9029..10786
                     /locus_tag="VHV99_03605"
     CDS             9029..10786
                     /locus_tag="VHV99_03605"
                     /inference="COORDINATES: protein motif:HMM:NF017589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytotoxic necrotizing factor Rho-activating
                     domain-containing protein"
                     /protein_id="HEX3634566.1"
                     /translation="MRINSSVAASYWQAPEPNNMARAGDERSARSGGARVKRSIPEDD
                     PAASPPKKEPPHNGPDEVRYVGGIRVRTDYTLEGVLETVKDALKHPIGAFAASVSDLH
                     HVVMHGRNITTQERKEIEEDTAQIDSVLVAIRGLTPGGGVMNVITVSADVLTAMSEDK
                     PLTLDQVHDLVFSINDLPSGESAEIPIRREDGAPAKAPSNRPDFNPPKRLPDGRTGYP
                     LSPTEPPKLPGESTGSGTSGHGARPKKARPSGKPQAGTSEPQPGTSRKGPPSTIKSTI
                     QGAFYSDANRVRKGDIAPLEGRGAIYLKAPGKNNTRFSFYRYDKDMKMSRPAHSSDIP
                     PAAAQEVLRTGNGRDVTSFASNSNSYFGQWGRGKRKLSEQIDVIELENGREGVGAVQI
                     SFADIPPKGAVLVTTGSLSGCTAMFAADNQKFYAYHTGTSTPNANWKTAREGVSSLRE
                     AHEHLKPDAQKKVVEQPGNNDLVDMANDYPFSVITYNGKFNKARPQDDTRINRPEWEW
                     SKGTHVNNYFEPDEKIPSIGTSVALIRKDARGRVSVSVLYEKGALKRAATKGRVGDPL
                     TYDYKAIETEKYKFTPQGS"
     gene            11049..12929
                     /locus_tag="VHV99_03610"
     CDS             11049..12929
                     /locus_tag="VHV99_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEX3634567.1"
                     /translation="MRRTPSSEPSPISTQPRNDWQTILSLLPYLATYKWRVGFALSCL
                     IGAKVANLGVPIVMKRIVDSLASVRHLTALGRAHDSPGIVLLGGIGLLVVAYAVVRLS
                     TSLFTELREILFAKVTESAVRQLALKVFRHLHALSLRFHLDRQTGGMSRDIERGTRGI
                     TQLISYSLFSILPTLVEVGLVLGFFVVKYEAYYAIVTFIALATYITFTVKVTEWRTHF
                     RRTMNDLDSKANSRAIDSLLNYETVKYFGNEEWEAARYDENLKRYRTAAIKSQRSLSA
                     LNFGQQAIIGTGLVFILWRATEGVMAGRLTLGDLVLINTFMLQLYIPLNFLGVVYREL
                     KQSLTDMDRMFTLLGAAQEVPDVPGAPELRVSGAQVRFEHVNFSYEPARQILHDVSFT
                     IPAGSTTAVVGHSGSGKSTLARLMFRFYDLDRATGGAITVDGQDIRDVTQDSLRASIG
                     IVPQDTVLFNDSIYYNIAYGRPSATREEVIAAARAAHIHDFIEGLPKGYETPVGERGL
                     KLSGGEKQRVAIARTILKNPPILLFDEATSALDSRSERAIQHELDQIARERTTLIIAH
                     RLSTVVHAQQIIVMDKGRIVERGTHAELLLADGLFAQMWALQQQRAAEAPESAETVHS
                     ER"
     gene            complement(13091..13804)
                     /locus_tag="VHV99_03615"
     CDS             complement(13091..13804)
                     /locus_tag="VHV99_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX3634568.1"
                     /translation="MTTSLKKAMIFAAGRGERMRPLTDTCPKPLLEVGGKPLIVWQIE
                     RLARAGFQTIVINHAWLGERIETALGDGSRWNVELRYSAEHEALETAGGIVQALPLLE
                     DEGSSEVFVAVSGDVYADFDYATLNTRAEALKASSEPGMHLVMVPNPAFHPGGDFGLI
                     DGVLSLDAQPRFTFGNIGLYDTRMVRGLPRGTRRALSPYYRETIARGLASGELYEGLW
                     ENVGTPAQLQALDQRLSAR"
     gene            complement(13808..14899)
                     /locus_tag="VHV99_03620"
     CDS             complement(13808..14899)
                     /locus_tag="VHV99_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX3634569.1"
                     /translation="MTLPPAKDPTPASSDSRLDLLKAWLNAHAARYALDLDTLAPASS
                     DASFRRYFRLAGKAAEGESASTLIAVDAPPPEKCREFVQIAQLLDAANVHVPRVLEVD
                     VDAGFMLVTDLGTDSYLGALTEAQSGDDPRRARTLMRDALDALLRWQLTSREGVLPPF
                     DEAFLRREMELMPEWFIGRHLGREIDDKTRGLLDRTFALLIASARAQPQVFMLRDFMP
                     RNLMIASAPNPVNPGVLDFQDAVVGPISYDVASLLRDAFLGWDEEFELDCFVYYWERA
                     KKAGLPVDPDFGEFYRQLEWMGLQRHIKVLGLFCRINYRDSKPHYMKDLPRFIGYARK
                     VAERYAPLRPFAKLLDELEGRANEVGYTF"
     gene            15167..17488
                     /locus_tag="VHV99_03625"
     CDS             15167..17488
                     /locus_tag="VHV99_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HEX3634570.1"
                     /translation="MPRKRRLVAALIAAPGLMPALAHAQLVGEAAQPQPIDAPWGMRL
                     APQLEEHPLQTGQRPATFVLGDSASGTIDQDLAAKGSAEVRHNTAVIKADALHYDQDT
                     DMADAYGQVHVINSGNTFIGPEAHLRVDSTEGFMTSPKYHFNLTGGSGSAERVDLLDS
                     ERSVFTKGTYTACACTDNPAWYIKGSEFDFDTGADEGVAYNSVLFFQGVPVFASPWLS
                     FPLSGARRSGILPPTFSLSSTNGLELSVPYYFNIAPNRDLLVTPRLISKRGVQLQSTF
                     RYLSPTYSGSITGEFLPDDHLTKTNRYALYIQHNQNFGNGFGGYIYYNRVSDNTYPED
                     LSSSANQFINGTQLLYQQEAGLTYNNGPWSVLAREQHWQTLTPSVAPYGREPQLNVKY
                     AKYNIGGFDFGAEADYSDFRITTADLTEGQRVLFNPYVSYSVVGPGYFVTPKVQWHFA
                     SYNLNNIGTDLPAGSVKNFTESIPTLSFDTGLIFERSVRVFGADYIQTLEPRLYYVYT
                     PFRNQNNAPLFDTADSDFGLAEIFQPNTFVGNDRIADANRLTAAITTRFINPATGDER
                     ARFVIAQQYFFQDQRVTLEPNQTSTQATHSDLIAGASFKLGAGFASETAFQYNADNNQ
                     LVKTSVGFGFSPASGKVINVAYRYTRANTTLDNQPINQMLVSGQWPLTHRMFGVARFN
                     YDLGGHRIVDGLVGLQYDADCWTLGAGIQRYANGLNTSGQQQSSTRFLAQLTFKGLSS
                     VDNGLMAAFRSSVAGYTPLPPPPPPESRFTSYE"
     gene            17556..>17801
                     /locus_tag="VHV99_03630"
     CDS             17556..>17801
                     /locus_tag="VHV99_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone SurA"
                     /protein_id="HEX3634571.1"
                     /translation="MAIMKKLRLATLAAGLAAVASFLSVAPVQAQALGGNSGQTVDTI
                     AAVVNNGVITRRELEERVGLITRRLNQQNAPIPPAEQL"
BASE COUNT         3445 a         5368 c         5540 g         3448 t
ORIGIN      
        1 tcagcgtatt tcaacgcaac gcaggacgct aaaccaatca cgcatcaaac agcttccgac
       61 tacaggctag gagtatcgtg aaagcaaaag atcttttttt ggccatcctg gtgacagcca
      121 tatggggtgc gaatttttca gtcatcaagc tcggcctaac ctcggtagat cccttcattc
      181 tcgcgggctt acgctttaca ttgtgtgccg ttcccgcagt gtttttcgtt cgtcggccaa
      241 atgtgagcat tggatatctg gcgagctacg gtctgctgtt cggggtggga ctctggggtg
      301 tcgttaatct cgggattcag gccggattgt ccgcgggcat cgcatcgctg gttctgcagt
      361 tcagtgcctt cttcactatc ctgctcggtt cggtcgtctt caaggaatcg atttcgaagt
      421 atcagtacat tgggatcgtc gtggcactgt tcgggctggc atccatcatc acgattaccg
      481 atgggtccgc gacgctgttg ggcgttgtgc tcgtgatcgt cggcgcattg tcgtggagca
      541 gtgcgaatat catcatcaag aaatccggta caaaggatgt gctggccttt ctcatctggt
      601 cgagcctttt ctcgccgatt ccgctattcg ccatggcgtt cctgcagcat ggtgcagcgg
      661 gttacgtcgc aacgttttcc cgcttcaatt ggacggcgat tttttccatt ctgtttcagg
      721 tgtatcccac gacgctattc ggttatggca tctggaacat gctgttaaaa aaatatccgg
      781 tgtcggcagt ggcgccgctt tctctcctgg tcccaatttt cggaatgttc ggctcgatgc
      841 tgattttcgg agaacacatt gggttgtcga aagtcctcgc gacgggcttc attgtgctgg
      901 gtctcgccat cggcctttac ggaaggcata tcggcggcat gattcgggcg cgggttcgcg
      961 cctgacgcga cacacacgag cgctctgctc cagccaggga attctgatga gcatgtttct
     1021 ggtcacaaca tttgtttatc gcttcgctaa cggtctgctt tttctcgcgg ttgcatggaa
     1081 tcttgtgcga tcgaccggag gaggtgcaat gccgttggcg ttgtcggcaa tagcgggctt
     1141 cttgccggcg gtactggtcg ctccgtttgc acgccggttg ctcgcgcata gggatatgcg
     1201 aaggatgacg atccacggag ttgcaggttt attcgcagcg gcgttggcct tcgcatttgt
     1261 tcagcgttgg tacgttggta tcctggtgat caatttcttt gtgctcgcga ttttctttct
     1321 cctggaaggt gcctgggacg gtttgctggc gtcgatcgcc aaaacgctgc cagtcgtaga
     1381 aggtgacaag ctcaattcgc ggcagagtgc ggcaacgcaa gcagggttaa tgttaggcgg
     1441 cctacctata ggaatactga cccgtgaagg aggggctgcg atgccgttta tcgcggcggc
     1501 aatgttgtac ctcgtgacga ttttgtgctt catgttgtta ccgttgcgtc gctgcgctca
     1561 gagtccgggt agtcccattg aatccgtcga ccagcaagag tcggaaattt cgcactctac
     1621 cgggccgatc atgtggtcga cgctagcaac gttggcattg gtctggccgt gcctggcctt
     1681 ggttaatatg gtggtcccgc tggtcgcgaa cagtcagggg aatgggacag tggaacatgc
     1741 ggcgatactc gatgcagcta ttggcttagg catgggcttc atcgggattg cgtatgagcg
     1801 gttgataagt ttgcgccgtc gtgcgtataa ccttctcata gtggggatgg cttttctgat
     1861 cccgttgccg tttgtcgtgc tgttgttgtt cccatatgca ttgtttccgc tggcggcatg
     1921 cttcttcatt tcaggcatag gctttggaat gttccgtatc agcgtgcgca agcaactgat
     1981 tacgacacaa cccgcatatc gtgtcggtca agttgttgca tcatgcaacg ggtatggcgt
     2041 tcctgtattg gctttagcag cgttgtttta cgcccagacc tggcgagtcg ggccggtgat
     2101 tccgttggcg gtcttcgctg tgttcggcgc gctagctacg ctaagcacaa aaggtggcgg
     2161 aagtctcggc cgtgctcaga gttccgcaac gccctcatgt cgaaccgagg ttacttaggt
     2221 accagacccg ggccgggttg cgtcgactgc aacgtgtcgt agtcgacgta gctggtttcg
     2281 cggcagaagc tgaccagccg gacgccggcc ttgcgggcaa tcgcaatcgc cagcgacgac
     2341 ggcgcagaaa tcgtcgcgac catcggcacg tcgacacgcg ccgccttgcg caccagctcg
     2401 tagctggcgc ggctcgacag aaacacaaaa ccttccttgg tatccgcgcg atcgagcacc
     2461 aactgaccga tcagcttgtc gagcgcgttg tgacggccga catcctcgaa cgcgtagcga
     2521 atcgcgcctg ccgcatcgca ccatgcggcg gcgtgcaggc cgccggtcag acgcgtcagc
     2581 gcctggtgtt ccggcagttc gcgcgcggca cgagcgatcg catccggcgc gagccgttgc
     2641 agaaagccgg tatcgggcac gcgctccggt tccagatcca gcagatcgat gctttcgatt
     2701 ccgcatacgc cgcagccggt gcgcccggcg agggcgcggc gcttttcctt cagcgcgacg
     2761 aaagcctgct gcaccacctt caattgcacc tcggcgtgcg gcaattcgtc atcgtcgtgc
     2821 agctcgactt caatgtcctg aatgtcgctg ccgcgttcca cgatcccttc cgaaatcgca
     2881 aaaccgaccg cgaacgcttc cagatcgcgc ggcgtgcaca tcatcaccgc gtgcgagatg
     2941 ccgttgaaga cgagcgccac cggccactcc tgaccgacat gatcggtgac ggattcgacc
     3001 gccgcgccgc gatgccggag tacgtggcgc tccacggtgc ccggctgccc ggctgtgtcc
     3061 agttcgttca agctgtttca ctcctgtcat gctgcgcgtt tgagcggctt gcagccttgg
     3121 ccggcgctgc gaagcggtgc cagaccacgc cgctattacg cgaataaggt tctaaaatac
     3181 ctcaagctgg gctggtgcgc tgcccgctac caaagaggaa tactgtcatg ggactcaacg
     3241 aagcaccgct tctgttcaac ttcgaagtcg cgtcttcgga gaatttcacc tacatcccga
     3301 tgtcggtgcg cttcaatctt gatcgcttcg ggctgcgcat cacgctcgcg cagtggcagt
     3361 tgctgccgct cgaggatcgc aagctgctgg cgcgtttccc ggtcgaagag gacgcggaaa
     3421 tcgaaccgaa tttcgaccat gcgctgttcg aaatgctgcg cacgcacgcc aacgtcgagc
     3481 cggagtggtt tacgcctgag gaagcgcccg cctggcgccg cacggatagc gtgccggatg
     3541 gcgtcgcgca tcaggcgggg ctggccggcc tgccgacgcc ggacgttgcc cagtgggccg
     3601 aactcgagcc gttcaaacgc tacgtcctcg ccaaattgtc gcgcaagccg gacggcaacc
     3661 acgacttcgt ccccgcgatg aaggaatttg gcgcggccgg ctaggcgcgg ccggctaggc
     3721 gcggccggct aggtgcagcc ggctaggcgc ggccagctcg gtgcggccgc ctaggcgctg
     3781 ccgctagacg cggcccggtt agaagcgccc ggttagatgc ggccggccgg gtccggccgg
     3841 tcaaaactgg ccgcccgaaa ctacgaacct gccgccatca gcccccaaaa cggtcgccga
     3901 acattcacgc gcggtcagaa gccgcgcggg caccctgcga ttttttcatc cctcgccctc
     3961 aatctgttcc gaatcctgcc gttatccatg ggttggcgcg taaaaagact cgactcgatg
     4021 cgacccgacg caccgcaagc gaccccgcgc tcccgccctc ggaacgattg acgttcggaa
     4081 cccatgactc tttttttatc gaagcggctg ctgatcaatc tggcggtcgt cgccgcggcg
     4141 atcggcgcga ccgcgtgcgt cgcctatacg cagatttccg gccagcgcga cgccgacgcg
     4201 cgcatgctgc gttcgacgag cgtgcgtcag aatctcgacg cctaccatac ggcgctcgac
     4261 ggcgggctgg cggcgctcgg ccatttcgaa gccacgggtc aggccgtgcc ggtaaacgag
     4321 gtcgccgcca tggcgcccgt gctggcgggt ctcgagcgtg atttgcgcaa cgaaatcgcc
     4381 ggcgaacccg cgatgctcgg cacgctcgcc aggctaagcg cggacagtca cgcgctgcag
     4441 cacgatatcg ccgatgcgct gttgaagagc gccagcgccg cgccggacga atcgcgcgcg
     4501 tgggcggcgt cgacctatac gcaactcggg ctgggtctgg gccgtgtgga agcaggtctc
     4561 gcggcgctgc gccagcagga ggaccaggcg ttgcaggcgt cggtcgcggc ttcgaccagc
     4621 gagacccaac gcgccatgtt tctgctgatc gtgacgatgc tgggcggcgc tacgctcctg
     4681 ctcttcacgt tcggcgcccg cgaaagcagc gcacgcgaga agctgcgcac ggtcggcgcg
     4741 ctcagccgca acgacgagcg ttttcgcggc ctgttcgatg atcatccggt gccgatgtac
     4801 attttcgaca gcgagacgct gcgctttctc gcggtcaacg cggccgcgat ccagcaatac
     4861 ggctactcgg aaagcgaatt tttcggcatg acgattcggg cgatccggcc ggatagcgaa
     4921 atctcgcggc tcgaatcgca cctgcagcgc agcgatgtcc tgcaacatgg ccgcacgatg
     4981 gcgggtatct ggcatcaccg gcgcaaggac ggctcaatga tcagcgccga catttcgtat
     5041 cactcgctca gcttcatggg ccgcgccgct ttcttcgtgc tggccgatga cgtcaccgag
     5101 cagatcaacg ccgaagccga agcgcagcgt tcgaaccaga tgctcgaaac ggtgatcgac
     5161 aacattccgc agcggatttt ctggaaggat caggagtcgc gctatctcgg ctgcaacatg
     5221 gcgttcgcgc gcgatgcggg actctcttac tctgagcagg tagtcggcaa gagcgacagc
     5281 gagatgccgt ggcgcgctta cgccgagctg ctcaacgagc acgataggga ggtggtgacg
     5341 accggcgtgc cgaagatgaa cttcgaggtc gatgtgctga tcgacggcgt gcatcgcacc
     5401 atggtcacga gcaagctgcc gttcaccgac agcgacggcc gcgtgatcgg cgtgctcgga
     5461 tcctatacgg acatcaccga gcgcaagcgc tccgatctgg ctttgcgtct gcaaagccgc
     5521 gcgctcgatg ccagcgtcaa cgcgattctg attaccgcgc cgtcgccggc gggcaatctg
     5581 atcgaatacg tgaaccccgc gttcatgcgc atcaccggtt acgaccccgc cgaagtgatc
     5641 ggccacgatt gccgtgtact gcagcgcgat gaccgcgacc aggaaggcgt cgcgttgatc
     5701 cgtcaggcgc tctctgcgaa ccgtgaggtg agcgccgtgc tgcgcaacta tcgcaaggac
     5761 ggcgcgctgt tctggaatca actgttcatc gctccggtgc cgaacgcgga aggcatgatc
     5821 acgcaccaca tcggcgtgat caacgacgtg accgatctga tccgctatca ggaacagctt
     5881 gagtatcagg ccaattacga cagtctcacg cggctgccga atcgcaatct gctgcgcgat
     5941 cgcctgcagc atgcgttgat tgtggcgcag cggcatcaca agggcgtcgc ggtcgtgttc
     6001 atcgatcttg acggcttcaa gaacgtcaac gatacccttg gtcatagcgt cggcgaccgc
     6061 ttgctgagtg tggtggccga gcgcctcgcg cgctgctcgc gaaccagcga cacggtcgcg
     6121 cggcacggcg gcgacgagtt cgtgatcgtg atgaccgata ccgtcgacga gcaatcgctg
     6181 atcgcgtgga tggagcgggt gcgcgcgtcg atttccgagc cggtatggct cgacggcacc
     6241 gagttgtatg tcggctgcag catgggcgcg agcctctttc cgcaggacgg cgaggacgcg
     6301 gaaacgctga tgaaaaaagc cgacctcgcg atgtatcgcg cgaaggacat gggccgcaat
     6361 actttccagt tctatcagcc ggagatgaat gcgagcgtag gcgcgcgcct gaatctcgag
     6421 ctgcgtttgc gccgcgcctt gcgcgacaac gagttcttgt tgtactacca gccgcaggtg
     6481 gatatcggga gcggacagat cgtcggcacc gaggcgctgg tgcgctggcg cgatccggaa
     6541 gccgggttgg tgctgccgtc gttattcatt ccggttgccg aggagagcgg cttgatcggg
     6601 ccgttgtcgg aatgggtgct gcgtgaggcg tgccgccaga acaaggcgtg gcaggacgaa
     6661 ggcttgccgc cggcgcgggt ctcggtgaat ctgtcggcgc gcgtgtttca gcagcgcgat
     6721 atcgccaagc tcgtcaaaca ggtgctggcc gagacgggtc tcgagccgca gtatcttgaa
     6781 ctcgagctga ccgaaagcac catcatgcgc aatgccgagg aggcggtgtc gatgctcaac
     6841 gagctgcacg cgctcggcat cggccttgcc atcgacgact tcggcaccgg ctattcgagc
     6901 ctcggctatc tgaagcgctt tccggtggac cggctgaaga tcgaccgctc gttcgtgtcg
     6961 gatatcggcg tgtcgggcga cgatgaaacg attacctcgg cgatcatcgc gctcgcgcat
     7021 tcgctgaagt tgcaggtgat cgcggaaggc gtggagacct tggcgcaact cgactttctg
     7081 aaagagcgtg catgcgacga gatgcaaggc ttctacttcg cgaggccgct gtcgacggat
     7141 gcgatctccg agttgttcca ggggggcgtg gaccgggaag tggcgacggt gtaacccgtg
     7201 gcgatgcagc gctacgcgag actcgcgaag cgctgcggca accgatcaga cggtgtttgt
     7261 gcagtattaa gccagtaagg cgttcgctag gtcaaaacag cgacattaat cgacgatttg
     7321 gccggtcttt cgccgtgtaa agcggtttcc gcgcgagtgt cggtcgtata tattcgagtt
     7381 tcccatctca gacttcgctg ggaaccacga agcaccgatt cacgtggcgt tgcgcccgct
     7441 tttcgctgct cgcacgctgt catgggcgtt tcgccgtcga ctatccgcca attcgactgg
     7501 agagacaaat gacatcttca cctgcaagga catgggctgt gctgctggca tcggttgtac
     7561 tgagcgtggc ttcggtcgca ccggcgttcg cgcaaagcgg cggtggcggt ggcccggcgg
     7621 gggatgacgc cgatgctgcc aaacccgtgc ctgcttcaaa ggcagccacc acgaaggccg
     7681 agcgcagtgc cgcggccaag cagagacggg cgaagaagaa cgccgagttg aagcagttgg
     7741 agagtaacgg atataacccg tcgcgcaaca atccggacta cccgactgat ctccagaacg
     7801 cgcagaagaa ggcggggata ggcgcagcgg cgagccaata aacacgagcc aataaacggc
     7861 tgcgggccgg acggaggctg ccggctcgat cgtgttgacg ctgggagatt ccggcaaacg
     7921 caacgtggca aacgccgcct cgcaagcgca atccggcaca cacggccctg gcagcgcacc
     7981 ctgcgcaaac acagcccgcc acgcacaacg tatgaagata aacgaggcgc tcaggcgcct
     8041 cgttttcatt cagtgtccgc cgctcaatcc agcaccggca gcgcgcgcgg gcgccggtcg
     8101 ctatcggtcg cgacataggt cagggtcgct tcggtgacct tcacgaggtc ttcggtcagg
     8161 ctcatgcgct gcgcgtagac ctcgaccgcc accgtcaccg acgtgttgcc cgtcttgacg
     8221 atatcggcgt aaaaactcag cagatcgccg acaaacaccg gctgcttgaa gacgaacgaa
     8281 ttgaccgcaa tggtcgccac gcgcccgttg gcacggcggc ttgccggaat cgaaccggcg
     8341 atatccactt gcgccatgat ccagccgcca aacacgtcgc cgtggacatt cgcgtccgac
     8401 ggttgcggca tgacgcgcag cgcgcaaggc ttttgcggaa gttgaagcgt atcggtcatc
     8461 ggggcaccct tgaaattcgt aatggctcgg ccggctcgac cagcaagcgg gggatttcac
     8521 gaagactgca cgacacagcg gtacgacaca gcggtacgac acagcggtac gacacagccg
     8581 tacgacacag ccgtacggca tggctgcgga gcatagccgc ccggcacagc ggcacggccc
     8641 gcatgaaacc acgggcggcc atcccatgaa agcggcgcca gccggcttct gcgacagtaa
     8701 agatcaggaa ttgtacggga aagcgcctag gcagggcgcg tacctgaaag caggctggtt
     8761 cgctgccagt cggcgtgccg ctgataacac atgatccgcc gggaatcgca gacgcgcgtt
     8821 cctgttgctt ctgtttcgac gatctcgctg cgaagtggcg ctgcgaagta cgggggtaac
     8881 accctccgca aaaacatcag cgcacgatgg cggttcccgt gcggattgcc tcgttccgga
     8941 tatccggggc gctgcagcgg atggcgattt cgagccagac aaaacgagcg gtgcgcacgt
     9001 tccgaaatct gacgacgcga gattatcgat gagaatcaac agcagtgttg cagcgtccta
     9061 ttggcaagcg ccagagccga acaatatggc gcgcgccggc gatgaacgaa gcgcgcgctc
     9121 cggtggcgca cgcgtgaagc gttctatacc tgaagacgac ccggcagctt cgccgccaaa
     9181 gaaggagccg ccccataacg gcccggatga ggtccgatat gtggggggca ttcgcgtcag
     9241 aaccgattac acgctggaag gtgtgctgga aaccgtcaag gatgcactta agcacccgat
     9301 cggcgcgttt gccgcgagtg tcagtgatct gcaccacgtc gtgatgcatg gcaggaatat
     9361 caccactcag gaaaggaagg agatcgagga agataccgca cagatcgatt cggttctggt
     9421 ggccattcgc gggttgacgc cgggcggcgg cgtgatgaat gtcattactg tttccgctga
     9481 tgtgctcacc gccatgtccg aagacaagcc gttgacgctg gatcaggtcc acgatctggt
     9541 cttcagtatc aacgatttgc cttccggaga gagcgctgaa atacccatca gacgagagga
     9601 cggtgcgcca gcgaaagcgc cgtcgaaccg tccggatttc aatccgccga agcgactgcc
     9661 ggatggccgt acgggctatc cgctgagtcc aaccgagccc cccaaattac caggcgagtc
     9721 gacgggatcc ggcacaagcg ggcatggggc gcggccgaaa aaggcgcgcc cctccgggaa
     9781 gccgcaagcc ggaacaagcg agccgcaacc gggcacgagc cggaaagggc caccctctac
     9841 catcaagtcg acaatccagg gggcgttcta tagcgatgcc aaccgggtca ggaaaggcga
     9901 tattgcgccc cttgaaggga gaggcgccat ttacctcaag gcgccgggca agaacaacac
     9961 caggtttagt ttttatcgtt acgacaagga tatgaaaatg tccaggccgg ctcattcgtc
    10021 ggatatccca cccgctgcgg cgcaagaagt tctgcgaacg ggcaatgggc gggacgttac
    10081 ttcttttgct tcaaactcga acagttattt tggtcaatgg ggacgaggca agcgcaagct
    10141 gagtgagcag atcgatgtga tcgaacttga aaacggccgg gaaggtgtgg gtgctgtaca
    10201 aatctctttt gcggatatac cgcccaaggg ggcggttctg gtgacgacag ggagtctcag
    10261 cggatgtacc gcgatgttcg ctgcggataa ccagaaattt tatgcttacc acaccgggac
    10321 atcgactccg aatgccaact ggaaaaccgc tcgggaaggc gtgagcagtc tgcgcgaggc
    10381 gcacgaacat ttaaaaccag atgcgcaaaa gaaggttgtg gagcaacccg gtaataacga
    10441 tctggtcgat atggcgaatg actatccctt ttccgtgatt acctataacg gaaaattcaa
    10501 taaggcccgt ccgcaggacg acacgcgtat taatcgtccg gaatgggagt ggtcgaaagg
    10561 aacccacgtc aacaattatt ttgaacccga tgaaaaaata ccgtcgatag gaacgagtgt
    10621 tgctttgatt cgaaaagatg cacgaggacg cgtctctgtc agcgtgcttt atgaaaaagg
    10681 tgcactgaaa agagccgcca ccaaggggcg cgtcggcgat cccctcacgt acgactacaa
    10741 agcaatagag accgaaaaat ataagttcac cccgcaaggc agttgacggg accgggcata
    10801 cggttggggg cgaagtactg ctgttgccga ggtgcttctg gaaaaggtct ggcagccggc
    10861 atggcaagac cgcgaccggc acaagccgga cattccggga tacggtagac tgatccgcaa
    10921 tgacaagacg cttaagccat ccgcagtgaa ggcgggcgcc gcggacttcg ctggagcgcg
    10981 tctgacctgt gtctgtctga cctgtatatg acggttccgt cgctttcaca ctctccccag
    11041 ccgatcccat gcgccgtacg ccctcatccg aaccttcacc gatctcgacc cagccgcgca
    11101 acgactggca aacgatcctg tcgctgctgc cttacctcgc cacctataaa tggcgcgtcg
    11161 gcttcgcgct gagctgcctg atcggcgcga aagtcgccaa tctcggtgtg ccgatcgtga
    11221 tgaaacggat cgtcgacagc ctcgcgtcgg ttcggcatct caccgcgctc ggccgcgcgc
    11281 acgattcgcc cggcatcgtg ctgctgggcg gcatcggtct actggtggtc gcctatgcgg
    11341 tggtgcggct gtccacctcg cttttcaccg agctgcgcga gattctgttt gcgaaagtca
    11401 ccgaaagcgc ggtgcgccag cttgcgctga aagtgttccg ccatttgcat gcgctgtcat
    11461 tgcggttcca tctcgaccgc cagacgggcg gcatgtcgcg cgatatcgag cgcggcacgc
    11521 gcggcattac ccagctgatt tcgtattcgc tgttcagcat cctgccgacg ctggtcgaag
    11581 tcggtctcgt gctcggcttt tttgtcgtca aatacgaggc gtattacgcg atcgtcacgt
    11641 ttattgcgct cgccacgtac atcacgttca cggtgaaagt caccgagtgg cgcacgcatt
    11701 tccgccgcac gatgaacgat ctcgattcaa aggcgaattc gcgcgcaatc gattcactgc
    11761 tcaactacga gacggtcaaa tacttcggca acgaagagtg ggaggcggcg cgttacgacg
    11821 aaaacctcaa acgctatcgc acggccgcga tcaaatcgca gcgctcgctg tcagcattga
    11881 acttcgggca gcaggcgatc atcggcactg ggctcgtgtt cattctgtgg cgcgcgaccg
    11941 aaggcgtgat ggctggccgc ctcacgctcg gcgatctggt gttgatcaac accttcatgt
    12001 tgcagctcta tattccgctc aattttctcg gtgtcgtgta tcgggaactg aagcaaagcc
    12061 tcaccgatat ggaccgcatg ttcacgctgc ttggcgcggc ccaggaagtg cccgatgtgc
    12121 ctggcgcgcc cgagttgcgg gtgagcggtg cgcaggtccg cttcgagcat gtgaactttt
    12181 cgtatgagcc ggcgcggcag attctgcacg acgtgagctt cacgattccg gcgggcagca
    12241 ccacggcggt ggtcggccac agcggctcgg gcaaatcgac gctcgcgcgg ctgatgttcc
    12301 gcttctacga tctggaccgc gcaacgggcg gtgcgatcac cgtcgacggt caggatattc
    12361 gcgacgtcac gcaggattcg ctgcgggctt cgatcggcat cgtgccgcag gatacggtgc
    12421 tgttcaacga ttcgatctac tacaacattg cttatggccg tccgtcggcc acgcgcgagg
    12481 aagtgatcgc ggcggcgcgc gcggcgcata ttcacgactt catcgaaggg ttgccgaagg
    12541 gttacgagac gccggtcggc gagcgcggcc tgaagttgtc cggcggtgaa aagcagcgtg
    12601 tggcgattgc gcggaccatc ctcaagaacc cgccgatcct gctgttcgac gaggcgacgt
    12661 cggcgctcga ttcgcgttcg gagcgcgcga tccagcatga gctcgaccag atcgcccgcg
    12721 agcgcacgac gctgatcatc gcgcaccgat tgtcgacggt ggtgcacgcg cagcagatca
    12781 tcgtgatgga caaggggcgc atcgtcgagc gcgggacgca tgctgagttg ctgcttgcag
    12841 acggcctgtt cgcgcagatg tgggcgttgc agcagcagcg tgccgcagag gcgccggagt
    12901 cggctgaaac ggtgcatagc gagcgctgag cgagcgttga gcaaacactg agcgaacact
    12961 gagcgaacac tgagcgaacg gcggcgggac gcactgctgg gcgatcggcg ttgaccgccc
    13021 agttttgacc atgacacgtt gcgccgcgcg cgtcattccg ctgcgtcgtt cagcgccatg
    13081 cggcctgcga tcagcgtgcg ctcaaacgct gatccaaagc ctgcagttgc gccggcgtac
    13141 cgacgttctc ccataggcct tcgtacaact cgccgctcgc gaggccgcgt gcgatcgtct
    13201 cgcggtaata cggcgacagc gcgcgccgcg taccgcgcgg cagaccgcgc accatgcgcg
    13261 tgtcgtagag gccgatgttg ccgaacgtga agcgcggttg tgcatcgagc gacagcacgc
    13321 cgtcgatcaa accgaagtcg ccgccaggat ggaaggccgg gttcggcacc atcaccagat
    13381 gcatgccggg ctcgctcgac gctttcagtg cttcggcgcg cgtgttcagc gtcgcgtaat
    13441 cgaaatccgc gtagacgtcg ccgctcactg cgacgaacac ttcgctgctg ccctcgtctt
    13501 cgagcagcgg caacgcctgc acgataccgc ccgcggtttc cagcgcttca tgctcggccg
    13561 agtagcgcaa ttcgacgttc cagcgcgaac cgtcgccgag cgcggtttcg atccgctcgc
    13621 cgagccacgc gtggttgatc acaatggttt gaaaaccggc gcgtgcgagg cgctcgatct
    13681 gccacacgat caacggcttg ccgcccactt cgagcaaagg cttcgggcat gtgtcggtca
    13741 acggacgcat gcgctcgccg cggcccgccg cgaaaatcat cgctttcttc agagacgtcg
    13801 tcatcggtca gaacgtgtag ccgacttcat tggcgcggcc ttcgagttcg tcgagcagct
    13861 tcgcgaacgg gcgcagcggc gcatagcgtt ccgccacctt gcgcgcatag ccgatgaaac
    13921 gcggcagatc cttcatgtaa tgcggtttgc tgtcgcggta gttgatccgg cagaagagcc
    13981 cgagcacctt gatgtgccgt tgcaggccca tccattcgag ctggcggtag aactcgccga
    14041 aatccgggtc gaccggcagg cccgcttttt ttgcgcgctc ccagtagtac acgaagcaat
    14101 ccagttcgaa ttcttcgtcc cagccgagga acgcgtcgcg caacaacgag gcgacgtcgt
    14161 aactgatcgg gccgaccacc gcgtcctgga agtccagcac gccgggattc actggattcg
    14221 gtgcgctggc gatcatcaga ttgcgcggca tgaaatcgcg cagcatgaac acctgcggct
    14281 gcgcccgggc gctcgcgatc agcagcgcga aggtgcgatc gagcagcccg cgggtcttgt
    14341 cgtcgatctc gcggcccaga tggcggccga tgaaccactc cggcatcaac tccatctcgc
    14401 ggcgcaggaa ggcttcgtcg aacggtggca gcacgccttc gcgcgacgtg agctgccagc
    14461 ggagcagcgc gtcgagcgcg tcgcgcatca gcgtgcgggc gcggcgcggg tcatcgccgc
    14521 tttgcgcttc agtcagcgcg ccgaggtacg aatcggtacc cagatcggtg acgagcatga
    14581 acccggcgtc gacatcgacc tcgagcacac gcggcacatg cacgttggcg gcgtcgagca
    14641 attgcgcgat ttgcacaaat tcgcggcact tttcgggcgg cggcgcatcg acggcgatca
    14701 gggtgctggc gctttcgcct tccgccgcct taccggcaag ccggaaatag cggcgaaaac
    14761 tggcgtcgga cgaagccgga gcaagcgtgt ccaggtcgag cgcatagcgc gctgcatggg
    14821 cgttcagcca ggccttgagc agatcgaggc ggctgtcgga ggaagctggg gtagggtctt
    14881 tggcaggggg cagcgtcatg aaaaccggcg gcaaattctg aggggcaacg tcttgccata
    14941 taatacccca cgactttttt gacgcgactc acgccagctt tcgcgtgttc cgctttaccg
    15001 cccgcctcat cgttcgacag attgtaaata ttgatgtaca gccgactgtc acggtcgcga
    15061 tgtgcaggcg gtggatcgtg actgcaaaag ctgacggacg ggccgattcg ccaaacgata
    15121 catgccgcct agacagcttt cccaaacgac tctctcttgt gccgtaatgc cgcgcaaaag
    15181 gcggctcgta gcggcgttga tcgccgctcc gggcctgatg cccgcgcttg cgcacgccca
    15241 gttggtgggg gaagccgcgc agccgcagcc tatcgatgcg ccatggggca tgcggcttgc
    15301 cccgcaactc gaagaacatc cgctgcaaac cggccagagg ccggccacgt tcgtgctcgg
    15361 cgacagcgcg agcggcacca tcgaccagga cctggcggcc aagggttcgg ccgaggtgcg
    15421 ccacaatacc gccgtgatca aggccgacgc gctgcactac gatcaggaca cggacatggc
    15481 cgacgcgtac ggccaggtgc acgtgatcaa cagcggcaat accttcatcg gtcccgaagc
    15541 gcatctgcgc gtggactcga ccgaaggctt catgacctcg ccgaaatacc acttcaacct
    15601 gaccggcggc tcgggcagcg cggagcgtgt cgacctgctc gactccgaac gctcggtgtt
    15661 cacgaagggc acttacactg cgtgcgcttg cacggacaac ccggcgtggt acatcaaggg
    15721 cagcgagttc gacttcgaca ccggcgcgga cgaaggggta gcgtacaaca gcgtgctgtt
    15781 cttccagggc gtgccggtgt tcgcgtcgcc gtggctgtcg ttcccgctgt cgggcgcccg
    15841 gcgcagcggg attttgccgc ccacgttctc gctgagttcg accaacggcc tcgaactgtc
    15901 ggtgccgtat tacttcaaca tcgcgccgaa tcgcgatctg ctcgtcacgc cgcgtctgat
    15961 ctcgaaacgc ggtgtgcagt tgcagagcac gttccggtat ctgtcaccca cgtattccgg
    16021 ttcgatcacc ggcgagttcc tgccggacga ccatctgacc aagaccaacc gctacgcgct
    16081 gtacatccag cacaaccaga acttcggcaa cgggttcggc ggttacatct actacaacag
    16141 ggtttcggat aacacctatc cggaagacct gtcgtcgtcg gccaatcagt tcattaacgg
    16201 cacccagctc ctgtatcagc aggaagccgg gttgacctac aacaacggcc cgtggtccgt
    16261 gctcgcgcgc gagcagcact ggcaaacgct gacgccgtcg gtcgcgccgt atggccgcga
    16321 gccgcagttg aacgtgaagt acgcgaagta caacatcggc ggtttcgact tcggcgccga
    16381 ggccgactac tcggacttcc gcatcaccac cgcggacctg accgaaggcc agcgggtgct
    16441 gttcaacccg tacgtgtcgt attcggtggt cgggccgggc tacttcgtca cgccgaaggt
    16501 gcagtggcac ttcgcgtcgt acaacctgaa caacatcggc accgatctgc cggccggctc
    16561 ggtgaagaat ttcaccgagt cgatcccgac gctgagcttc gacacggggc tgattttcga
    16621 gcgttcggtg cgcgtgttcg gcgcggatta catccagacg ctggagccac gcctgtacta
    16681 cgtgtacacg ccgtttcgca accagaacaa cgctccgctg ttcgacaccg ccgactccga
    16741 cttcggtctg gcggagattt tccagccgaa caccttcgtc ggtaacgacc ggatcgccga
    16801 cgcgaaccgt ctgacggccg ccatcaccac gcgcttcatc aacccggcca cgggcgacga
    16861 acgcgcccgc ttcgtgatcg cgcagcagta ctttttccag gaccagcgcg tcacgctgga
    16921 gccgaaccag accagcacgc aggccacgca ctcggacctg atcgcgggtg cgtcgttcaa
    16981 gctcggcgcc ggtttcgctt cggaaacggc gttccaatat aatgccgaca acaatcagct
    17041 ggtgaagacg agcgtcggct tcgggttcag tccggcgagc ggcaaggtga tcaacgtcgc
    17101 gtatcgctac acgcgcgcga acaccacgct ggacaaccag cctatcaacc agatgctggt
    17161 ttcggggcaa tggccgctca cgcatcggat gttcggggtt gcgcggttca attacgatct
    17221 gggcgggcat cggatcgtcg acggtctcgt gggtctgcaa tacgacgccg actgctggac
    17281 gctgggcgcg ggtattcagc gctacgcaaa cggcctgaat acgtcggggc agcaacagtc
    17341 gagcacgcgc tttctcgcgc agttgacgtt caagggcttg tcgagcgtcg acaatgggct
    17401 gatggcagcg ttccgcagca gtgtcgcagg ctacacgccg ctgccgccac cgccgccgcc
    17461 ggagtcgcgg ttcaccagtt acgaatgacg ctcggccggt cgttcatcga atgcgcaaag
    17521 acgggccaga cgtgcccgac atcattggag tatctgtggc aatcatgaaa aagcttcgct
    17581 tggcaacgct cgcggcaggt ctcgccgccg tggcgtcttt cctgtcggtt gcgcccgtac
    17641 aggcgcaggc gctgggcggc aacagcggcc agacggtcga cacgatcgcc gcagtggtca
    17701 acaacggtgt catcacgcgg cgcgagctcg aagagcgcgt gggcctgatc acccgccgcc
    17761 tgaaccagca gaacgcgccg attccgccag ccgaacagtt g
//
LOCUS       DATASH010000122         3767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5555468,
            whole genome shotgun sequence.
ACCESSION   DATASH010000122 DATASH010000000
VERSION     DATASH010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5555468"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..126
                     /locus_tag="VHV99_03635"
     CDS             <1..126
                     /locus_tag="VHV99_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3634572.1"
                     /translation="LGRAHGLPEITALEMVLARSTASKRPPCDFLAEKILAELRR"
     gene            309..1577
                     /locus_tag="VHV99_03640"
     CDS             309..1577
                     /locus_tag="VHV99_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX3634573.1"
                     /translation="MSEAVSKQPKRSRRGLIIFAVIVLALIALVAVHLLTQKKPSRET
                     APIVVTVASATLGSMPVTLSELGTVTPIATVTVLPQLSGYLTAVGYHEGQEVQKGQFL
                     AQIDPRQYQISKQQAQATLAKDRATLAQARADLVRYTQLNEHKSIAQQTFADQQFLVQ
                     QDEAAVESDLANIAQFDLDLAYCRITAPVAGKVGLRLVDPGNYVTASSSTGIVVITTV
                     KPTTVQFTVPQDSLASIMERLNGGATLPVTAYSSDNAREVATGTLYAVSNQMATATGT
                     VTLRATFANDDEALFPNEFVNVKLLVDTMQNAVLVPTAAVQTGAPGDFVYLVNANNTV
                     SVHKVKIGPSDGKNTVIVSGLSQGNEVVTDGTDRLNDGARIRPSKPGSAPAGAASGAS
                     ARRRSRGAAAEPAGSGSAAASAASADAASS"
     gene            1593..>3767
                     /locus_tag="VHV99_03645"
     CDS             1593..>3767
                     /locus_tag="VHV99_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX3634574.1"
                     /translation="MNISRLFILRPVATLLLMIALVLVGLVAMRVLPVSSLPNVDYPT
                     IQVQTFYPGASPDVMATTVTAPLEVQLGEIPGLQQMTSYSSDGASVITLQFDLSLNLD
                     IAEQDVQQAINASNSYLPAGLPAPPTYAKVNPADQPILTLAVTSKSMSLTQLEDVANN
                     RLGTKISEVSGVGVVTTSGGNVPAIRVEADPQKLAAYGLNIDDLRTLLSYVNVSQPKG
                     NFDGPDLDYTINGNDQITEPKDYLDTVIAYRNGAPVFMRDVARVSQAAQDVERGAWYN
                     GSPAIVLNVQRQPGANVIATVKQIMAELPQLESTLPAGMKVTVVSDSTGVIRASVADA
                     AFELILAIVLVVAVIFVFLRNVPATLIPSVSVPVSLIGTLAVMYQLNYSIDNLSLMAL
                     IIATGFVVDDSIVMIENIVRYLEEGKTPLEAALEGAGQIGFTILSLTISLIAVLIPLL
                     FMGGVIGRLFSEFAVTLAVTIVISAIVSLTVVPMLCARMLRAQAERHPSRFERISEGL
                     FDKTLSAYERGLRWVLDHQVLTLVVAIGTVVLTGVLYVVIPKGLFPVQDVGVIEGISV
                     ADNSVSYAAMVNRQGALADAILKDPDVTSLTSYVGIDGTNPTLNNGRFLINLRDRDKR
                     ADTAQEVARRLQQEVANVPGVRLYMQPEQDLTLDTTVSPNQYSFVLRGPSQQAFQKYV
                     PELIARMKQIPSLADVQSDMNSDGLSVNVEVNRQLAARFGITP"
BASE COUNT          707 a         1219 c         1163 g          678 t
ORIGIN      
        1 cttggacgcg cgcacggctt gcccgaaatt accgcgctcg agatggtgct tgcgcgaagc
       61 acagcctcga agcggccgcc ttgcgatttt ctggcggaga agattctcgc tgagttgagg
      121 cggtagcggt ccgccggtcg cgagtgatgc ggtttgcgta gtccgcctgg cttgcaatta
      181 gataacacca tccaaaaggc cgttgatttg tgacaacgca ccagaaccgt ggcatgttct
      241 cccaaaaacc aacaaagcat cacgccccgc ctgatcatca acggcagcat ctgttttcgg
      301 agagtcctat gagcgaagcc gtgtccaagc aaccaaagcg ttcccgacgc gggctcatta
      361 tctttgcggt tatcgtgctg gcgctgatcg cgcttgtcgc tgtccatctg ttgacgcaga
      421 agaaaccctc acgcgaaacg gcgcccattg tcgtcaccgt ggcgagcgcg acactcggtt
      481 cgatgccggt tacgttgagc gaactcggca ccgtcacgcc gatcgccacc gtcaccgtgc
      541 tgccgcagtt gagcggctac ctcacggcgg tcggttatca cgaaggccag gaagtgcaga
      601 aaggccagtt cctcgcgcag atcgatccgc gccagtatca gatcagcaag cagcaggcgc
      661 aggcgacact cgccaaagac cgcgcgacgc ttgctcaggc gcgcgccgat ctggtccgct
      721 atacgcagtt gaacgaacac aagtcgatcg cgcagcagac cttcgcggat cagcaattcc
      781 tcgtgcagca ggacgaggcg gcggtcgagt ccgatctcgc caacatcgcc caattcgacc
      841 tcgacctggc ctattgccgg atcaccgcgc cggtcgccgg caaagtcggc ctgcgccttg
      901 tcgacccggg caactacgtg acggcgtcat cgtcgacggg gatcgtggtc atcaccaccg
      961 tcaagccgac caccgtgcag ttcacggtac cgcaggactc gctggcctcc atcatggaac
     1021 gtctgaatgg cggcgcgacc ttgccggtca ccgcctatag cagcgacaac gcgcgcgaag
     1081 tggccaccgg cacgctctac gccgtcagca accagatggc cacggcgacc ggcaccgtca
     1141 ccttacgcgc gactttcgcc aacgacgacg aagcgctgtt ccccaacgag ttcgtcaacg
     1201 tcaagctgct ggtcgatacg atgcaaaacg ccgtgctggt gccgaccgcc gccgtgcaaa
     1261 ccggcgcgcc gggcgacttc gtctatctgg tcaacgcgaa caacacggtc tccgtccaca
     1321 aggtcaaaat cggcccgagc gacggcaaga acacggtcat tgtctccggc ctttcgcaag
     1381 gcaacgaggt ggtcacggac ggcaccgacc gtctgaacga cggcgccaga atccggccga
     1441 gcaagcccgg atccgcaccc gccggcgccg cctccggtgc atccgccaga agaagatcaa
     1501 ggggtgccgc tgcggagcct gccggctccg gatccgctgc cgcctccgcg gcgtcggccg
     1561 atgctgcttc gtcgtagcag gctcgcgcgc cgatgaatat ttcccgtctg ttcattcttc
     1621 gaccggtagc cacgttgctg ctgatgatcg ccctcgtgct ggtgggcctc gtcgccatgc
     1681 gcgtgctgcc ggtttcatcg ctgcccaacg tcgactatcc gaccatccag gtgcagacgt
     1741 tctatccggg cgcgagcccg gacgtgatgg ccaccacggt cacggcgccg ctcgaggtgc
     1801 agttgggcga gatcccgggc ttgcagcaga tgacgtcgta tagctcggac ggcgcgtcgg
     1861 tcatcacgct gcagttcgat ctttcgctga atctcgacat cgccgagcag gacgtccagc
     1921 aggccatcaa cgcttcgaac agctacctgc ccgccggcct gccggcgcca ccgacgtatg
     1981 ccaaggtcaa tccggccgac cagccgatcc tgacgctcgc ggtcacgtcg aagtcgatgt
     2041 cgctgaccca gctggaagac gtcgccaaca accgtctcgg caccaagatt tccgaagtgt
     2101 cgggcgtggg cgtggtcacg acgagcggcg gcaatgtgcc ggccattcgc gtcgaagcgg
     2161 acccgcagaa gctggcggcc tatggcctga acatcgacga tctgcgcacg ctgctcagct
     2221 acgtcaacgt cagccagccg aagggcaatt tcgacggccc cgacctcgat tacacgatca
     2281 acggcaacga ccagatcacc gagccgaagg actatctcga cacggtgatc gcgtaccgga
     2341 acggcgcgcc ggtgttcatg cgcgacgtgg cgcgggtcag ccaggccgct caggacgtcg
     2401 aacgcggcgc ctggtataac ggcagcccgg ccatcgtgct caacgtgcag cggcaaccgg
     2461 gcgcgaacgt gatcgcgacc gtcaagcaga tcatggcgga actgccgcag ctcgaatcca
     2521 cgctgccggc cggcatgaag gtgaccgtgg tatcggacag cacgggcgtg atccgtgcgt
     2581 cggtcgccga cgcggcgttc gaactgattc tcgccatcgt gctggtggtg gcggtgatct
     2641 tcgtattcct gcgcaatgtg cccgccacgc tgattccgag cgtatcggtg ccggtctcgc
     2701 taatcggcac gctggcggtg atgtaccagc tgaactattc gatcgacaac ctctcgctga
     2761 tggcgctgat catcgccacc ggcttcgtgg tcgacgactc gatcgtgatg atcgagaaca
     2821 tcgtgcgcta tctggaggag ggcaagactc cgctcgaagc cgcgctggaa ggcgccggcc
     2881 agatcggctt cacgattctg tcgctgacca tctcgctgat tgcggtgctg attccgctgc
     2941 tcttcatggg tggcgtgatc ggccgtctgt tcagcgaatt cgcggtgacg ctcgcggtca
     3001 ccatcgtgat ctccgcgatc gtctcgctga ccgtggtgcc gatgctgtgc gcgcgcatgt
     3061 tgcgcgcgca ggccgaacgt catccgagcc gcttcgagcg gatcagcgaa gggctgttcg
     3121 ataaaacgct ttccgcgtat gaacgcggcc tgcgctgggt gctggatcac caggtgctga
     3181 cgctcgtggt ggcgatcggc acggtggtgc tgaccggcgt tctgtatgtg gtgattccaa
     3241 agggtctctt tccggtgcag gacgtcggcg tgatcgaggg gatcagcgtg gccgacaact
     3301 cggtgtcgta tgccgcgatg gtgaatcgcc agggcgcgct tgccgatgcg attctgaaag
     3361 acccggatgt cacctcgctc acctcctacg tggggatcga cggcaccaac ccgacgctga
     3421 acaatggccg ctttctgatc aatctgcgcg accgcgacaa gcgtgccgac acggcgcaag
     3481 aggtagcccg gcggctgcag caggaagtgg ccaatgtgcc gggcgtcagg ctctacatgc
     3541 agcctgagca ggatttgacg ctcgacacca cggtctcgcc gaaccagtac agcttcgtgt
     3601 tgcgcggacc gagccagcag gcgtttcaga agtacgtgcc ggaactcatc gcgcgcatga
     3661 agcagattcc gtcgctcgcc gatgtgcaaa gcgacatgaa tagcgacggc ctcagcgtga
     3721 acgtcgaagt caaccggcaa ctggcggcgc gcttcggcat tacgccc
//
LOCUS       DATASH010000123         2429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4763997,
            whole genome shotgun sequence.
ACCESSION   DATASH010000123 DATASH010000000
VERSION     DATASH010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4763997"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..789
                     /locus_tag="VHV99_03650"
     CDS             100..789
                     /locus_tag="VHV99_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3634575.1"
                     /translation="MKVLIIEDDAAIATNLYDFLESRGYQVDVASEGVMGLRLAITQH
                     WDAILLDLALPGLDGLTICRKLREEARRDTPVLMLTAKDTLDDKLQGFVHGADDYLIK
                     PFSLKEAGARLAALIKRYKGQVTQPVLSCADVRFDLATLAVERAGRPVKLPPKCLQLL
                     RILMQSPNRVFVRAELEADVWGEQSLDSDTLRTHVYTLRRALTAHGEDDVIETVHGLG
                     YRFVVSDADAS"
     gene            773..2080
                     /locus_tag="VHV99_03655"
     CDS             773..2080
                     /locus_tag="VHV99_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX3634576.1"
                     /translation="MRTRRSLRLKVALVFSALTILLLVVQALGVKALAEAQEERLIVA
                     LIEDDMASLIRSFLIDPKLIPPLDPRLGAHVSVAGAVPVTLPTSAKDLSEGAHEIIVD
                     DQEIHVAVAPFGDTKIFRVYNFSRYERSFKQVVDALMTGTGAFALLTIWLAFGLSGLL
                     VRQVAGFARQVEALRLGGSASINPGKYDEAEVIELVAAFNDYHSRITDMIEREKEFTG
                     NVSHELRTPLTAIKTSSELLEQDASISPKSRARLRQINRAADNMIELVNALLLFAREE
                     SAVDTDPVHLCHVIETALAPFSERLADKGVSVALCIDRDLQVKANRSALAIVLSNLVD
                     NATRYTDRGLIRFSYGDGTLHIEDTGPGIPTDALSHVFERFYRADSVAGSARGFGIGL
                     SIVKNICDWHGWRVELDSGPNKGTRVSLRLPLAAPTQHDIPRN"
     gene            complement(2141..>2429)
                     /locus_tag="VHV99_03660"
     CDS             complement(2141..>2429)
                     /locus_tag="VHV99_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634577.1"
                     /translation="LLVFIETVTGIGLRCTACALTALLSMAQPAHVDWQGGQKRTTST
                     VLSQLPRLAYVQRLSACWRAELGVVHCAPRWLDAGKQQRSAHVGIHGSVGT"
BASE COUNT          489 a          719 c          723 g          498 t
ORIGIN      
        1 caggcgccac tcaaatggtc atggccaggc gataccatgt cgcctgcgca ccgaaatgcg
       61 tgtcttgcga cactgcccat ttttcctgga agccgttcca tgaaagtatt gatcatcgaa
      121 gacgacgccg ccatcgcaac caatctctac gactttctgg aaagccgtgg ttatcaggtc
      181 gacgttgcgt cagaaggcgt aatgggcctg cgtctggcca ttacgcagca ctgggatgcc
      241 atccttctgg acttggctct gcccggtctg gacgggttga ccatttgccg caaattgagg
      301 gaagaagcac gccgggacac gcctgttctc atgctgacgg cgaaagatac gctcgacgac
      361 aaactgcagg gtttcgtcca tggcgctgac gactacctca tcaaaccatt ctcgctcaag
      421 gaggccggcg cacgcctcgc ggcgctgatc aagcgctaca aaggccaggt gacgcaacct
      481 gtcttgagtt gcgctgacgt gcgcttcgat ctggcgacgc tcgcggtcga gcgcgccggg
      541 cggccggtca agctgccgcc caagtgcctg cagttgctcc gcatcctgat gcaatcgccg
      601 aatcgggtgt ttgtccgcgc cgaactggag gcggacgtgt ggggcgagca atcgttggac
      661 agcgatacgc tgcgcacgca cgtttatacg ctgaggcgcg cattgacggc gcacggcgaa
      721 gatgacgtga ttgagacggt acacggtcta ggctaccggt tcgtggtgag cgatgcggac
      781 gcgtcgtagt ctgcgcctga aggtcgcgtt ggtcttttcc gcgctgacga tccttcttct
      841 cgtggttcag gcactcggtg tgaaggcgct cgctgaagcc caggaagaga ggcttatcgt
      901 tgcgctgatc gaagacgaca tggcgagtct gatccggagc ttcctgatcg acccgaaact
      961 cattccgccc ctcgatccac ggcttggcgc gcatgtatcc gtcgccggtg cggtccctgt
     1021 cactttgcct acttcagcga aggatctttc cgagggcgcg cacgagatca tcgtcgacga
     1081 ccaggagatc cacgtggcgg tcgccccgtt cggcgatacg aagatatttc gcgtgtacaa
     1141 cttcagccgc tacgagcgaa gcttcaagca ggtggtcgat gcgttgatga ccggtaccgg
     1201 cgcgttcgcg ctgctgacaa tctggcttgc gttcggttta tccggtctgc tggtgcgcca
     1261 ggtggcgggc ttcgcgcggc aggtcgaagc gctccggctc ggcggatcgg catcgatcaa
     1321 cccgggcaag tatgacgaag ccgaagtcat cgaactggtc gcggcattca acgattacca
     1381 cagccgcatc accgacatga tcgagcgcga aaaggagttc accggcaacg ttagtcatga
     1441 attacgcacc ccgctcaccg cgatcaagac gagcagcgaa ctgctagaac aggatgcttc
     1501 gatcagcccg aaatcccggg cgcggctgcg gcaaatcaat cgcgccgcag acaacatgat
     1561 cgagttggtc aatgcgctgc tgctgtttgc gagagaggaa tcagcggtcg acacggaccc
     1621 cgtacacctt tgtcatgtga tcgaaaccgc actggcccct ttttccgagc gtctggcgga
     1681 caagggtgtg tcggtcgcgc tctgcatcga tcgcgatctg caggttaaag cgaaccgctc
     1741 cgcacttgcg atcgtgctgt ccaacctggt tgacaacgct acgcgctaca ccgaccgtgg
     1801 gcttatacgc ttctcctatg gggatggcac gctgcatatc gaggacaccg gcccgggcat
     1861 tccgaccgac gcgttgtcgc acgtattcga gcgtttttac cgggcggatt cggtagccgg
     1921 ttcggcacgc ggcttcggca ttggattgtc gatcgttaaa aatatctgtg actggcatgg
     1981 ctggcgggtc gaacttgata gcgggccgaa taaaggtacc cgggtatcac taaggctccc
     2041 actcgcggcc ccgactcagc acgacatccc gcgaaattga caacgtcttg acgaaaactt
     2101 cacaagccgt tgcgtaggat ccaagtctgt ctccatctct tcaggtgcca acagacccat
     2161 gaatgccgac atgcgccgac cgctgctgtt tgcctgcgtc cagccaacgg ggtgcgcaat
     2221 gcaccacgcc gagttcggcg cgccagcatg cggacaatcg ctgtacatag gcaagtcgag
     2281 gcaattggct tagcaccgta ctcgttgtcc tcttttgccc gccttgccaa tcgacatggg
     2341 ctggttgcgc cattgacaac agtgcagtga gggcacaagc agtacagcga agaccaatgc
     2401 cggtgaccgt ctctatgaag accaacagg
//
LOCUS       DATASH010000124         3646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3577082,
            whole genome shotgun sequence.
ACCESSION   DATASH010000124 DATASH010000000
VERSION     DATASH010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3577082"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..135
                     /locus_tag="VHV99_03665"
     CDS             <1..135
                     /locus_tag="VHV99_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3634578.1"
                     /translation="PNQTPSIRRMISAVLAGYAWDESNPYVADPVRRLNALHEVCTQP
                     "
     gene            complement(245..1582)
                     /gene="guaD"
                     /locus_tag="VHV99_03670"
     CDS             complement(245..1582)
                     /gene="guaD"
                     /locus_tag="VHV99_03670"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007745215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HEX3634579.1"
                     /translation="MTQSAYRAQLLTFNGDPAQSSNAAVFNEDGLLIVEDGHVVTAGA
                     YATLASQLTPGTQVQEMRDKLIVPGFIDTHIHYPQTDMIASPAPGLLPWLNEYTFPTE
                     RRFTDPAYARDTASFFVEELLACGTTTALVYCTVHKESADALFTESEARNLRMVAGKV
                     LMDRNCPEFLRDTAQSGYDDSAELIGRWHNRGRQMYALTPRFAPTSTEAQLEACGVLA
                     GKHPDIFIQSHVAENTDEVKWVADLFPGHRSYLDIYDHYGLLRRRAVYGHCIYLDEED
                     RKRMAQTGTVASHCPTSNLFLGSGLFDFDKADEAGMPIALATDVGGGTSFSMLQTMNE
                     AHKVARLTGHHLTATRMFYLATAGAAEALDLADKVGTLKPKSEADFVVLDPQATPLLA
                     RRTARTESLEELLFAFALLGDDRAIYETYAAGKRVHRRDDVRQHAPRAAKIAA"
     gene            complement(1690..2736)
                     /locus_tag="VHV99_03675"
     CDS             complement(1690..2736)
                     /locus_tag="VHV99_03675"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEX3634580.1"
                     /translation="MNTTTVTPLLEKAARAPKAELHIHIEGSLEPELIFALAERNGVQ
                     LAYDSIEALRAAYAFTDLQSFLDIYYAGASVLLHEQDFYDMTMAYVERCLADNVIHSE
                     IFFDPQTHTERGVPIATVVAGIERALAAAEKRGMTSKLILCFLRHLSEEDALATFEEA
                     RPLFEQYKHRLIGVGLDSSERGHPPSKFERVFAQARALGLKLVAHAGEEGPPSYIYEA
                     LDLLKVDRVDHGVRSIEDPALVTRLADSRVALTVCPLSNLKLCVFDDLTKHTLKDLLD
                     RGVAVTVNSDDPAYFGGYVNANYFATIDALKLNDAEVYTIIRNSFEASFVTPEQRSEL
                     IAKLDAHWHQSGPH"
     gene            complement(2832..>3646)
                     /gene="xdhC"
                     /locus_tag="VHV99_03680"
     CDS             complement(2832..>3646)
                     /gene="xdhC"
                     /locus_tag="VHV99_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018434439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory protein XdhC"
                     /protein_id="HEX3634581.1"
                     /translation="DGMRSPHMRRLERFALGPSLGQCCGGAVILAFERLDVGDLGWIT
                     TLAKRVAAGQSTVRSVSFGPAPDAVMLSDPEPGVDAADCLLWDGAGFDDSGALLTETI
                     APGDFRVVLFGAGHVGAALVRVFATLPCTVRWVDERDAQFPPVETLHAPNVKIDPNDA
                     PDEAIDQAAPHTYFIVMTHNHARDLELAERILRRGDFAFFGMIGSHSKRKQFEHRLAA
                     RGIDPSQIARMKCPLGVDGIVDKSPEIIAISAAAQVLQAVEANAGAHAAQTV"
BASE COUNT          635 a         1123 c         1209 g          679 t
ORIGIN      
        1 ccgaaccaga cgccgtcgat tcgtcgcatg atcagcgcgg tgctggcggg ttatgcgtgg
       61 gatgagtcga atccgtatgt cgccgatccg gtgcggcggt tgaatgcatt gcacgaggtg
      121 tgcacgcagc cttgagctgc gcattgcacg ccataagcaa atgaaaacgg cgcgtccttc
      181 gagacgcgcc gttttttagt tcaaccgccc gaatcaactc aggcgtcagc gggcaaccgg
      241 agaatcaagc cgcgattttc gccgcccgcg gcgcatgctg ccgcacgtcg tcgcgacgat
      301 gcacgcgctt gcccgcggca tacgtttcgt agatcgcgcg atcgtcgccg agcagcgcga
      361 acgcgaacag cagttcttcg agcgactcgg tgcgcgcggt acggcgcgcg agcagcggcg
      421 tggcttgtgg gtcgagcacg acgaagtcgg cttccgactt cggcttcaac gtgccgacct
      481 tgtcggccag atcgagcgct tcggccgcgc ctgcggtggc gagatagaac atgcgcgtgg
      541 ccgtcagatg atggccggtc agccgcgcga ccttgtgcgc ttcgttcatg gtttgcagca
      601 tcgagaacga ggtgccgccg cccacatcgg tggccagcgc gattggcatg ccggcttcgt
      661 cggccttgtc gaagtcgaac aggccgctgc cgaggaacag gttcgaggtc gggcagtgcg
      721 aggcgaccgt gccggtttgc gccatgcgct tgcggtcttc ctcgtcgaga tagatgcaat
      781 ggccgtacac cgcgcggcgg cgcagcagac cgtagtgatc ataaatgtcc agataactgc
      841 ggtggcccgg gaacagatcg gcgacccatt tcacttcgtc ggtgttttcg gcgacgtggc
      901 tctggatgaa gatgtccgga tgcttgcccg ccagcacgcc gcaggcttcg agttgcgcct
      961 cggtcgaggt cggcgcgaaa cgcggcgtga gcgcgtacat ctggcggcca cgattgtgcc
     1021 agcggccgat cagctcggcg ctgtcgtcgt aacccgattg cgcggtgtcg cgcaggaact
     1081 ccgggcaatt gcggtccatc agcaccttgc cggccaccat gcgcagattg cgcgcttcgc
     1141 tctcggtgaa gagcgcgtcc gccgattcct tgtggaccgt gcaatacacg agcgcggtgg
     1201 tcgtgccgca agccagcagt tcttcgacga aaaagctcgc cgtgtcgcgt gcgtacgccg
     1261 gatcggtgaa gcggcgctcg gtcggaaagg tgtactcatt gagccacggc aacaaacccg
     1321 gcgccggcga cgcgatcatg tcggtctgcg gatagtgaat gtgcgtatcg atgaaacccg
     1381 gcacgatcag cttgtcgcgc atctcctgca cctgggtgcc cggcgtgagt tgcgacgcga
     1441 gcgttgcata cgcgcccgct gtgacgacat ggccgtcttc gacgatcagc aagccgtcct
     1501 cattgaagac cgccgcgttg gacgattgcg cgggatcgcc gttgaaggtc agcagttgtg
     1561 cgcggtaagc cgattgagtc atgggtgaaa ccgtctccgt atttgtgagg agctagacag
     1621 cgcggacggc ggcttgatgc tggttctgca atgaacccct gccgccgccg gctgctcttc
     1681 attggcccgt cagtgcgggc cgctttgatg ccagtgcgcg tcgagcttcg cgatcagttc
     1741 gctgcgctgc tcaggcgtga cgaacgacgc ttcgaagctg ttgcggatga tcgtgtagac
     1801 ctcggcatcg ttgagcttca acgcgtcgat ggtggcgaaa taattggcgt tcacgtagcc
     1861 gccgaaataa gccggatcgt cggagttcac cgtgacggcg acgccgcgat cgagcagatc
     1921 cttcagcgtg tgtttggtca ggtcgtcgaa cacgcagagc ttgaggttcg acaacgggca
     1981 cacggtcagc gcgacgcgcg aatcggcgag acgcgtgacg agcgccggat cttcgatgct
     2041 gcgcacgcca tggtcgacgc ggtccacttt cagcagatcg agcgcttcgt agatatacga
     2101 cggcgggcct tcttcgccgg catgcgcaac cagcttcaga ccgagcgcgc gtgcctgggc
     2161 gaagacgcgt tcgaacttcg acggaggatg accgcgttcc gacgaatcca gcccaacgcc
     2221 gatcaggcga tgcttgtatt gctcgaacaa cggccgcgct tcttcaaacg tggcgagcgc
     2281 atcttcttca gacagatggc gcagaaaaca cagaatcagc ttgctcgtca tgccgcgctt
     2341 ttccgcggcg gcgagtgcgc gttcgatacc ggccacgacc gtcgcaatcg gcacgccgcg
     2401 ctccgtatgc gtctgcggat cgaagaagat ttcgctgtga atcacgttgt cggcgagaca
     2461 gcgttccacg tacgccatcg tcatgtcgta gaagtcctgc tcgtgcagca gcacgctcgc
     2521 accggcgtaa tagatgtcga ggaacgattg cagatcggtg aacgcgtacg cggcgcgcaa
     2581 ggcttcgatc gagtcgtagg cgagctgcac gccgttgcgt tcggcgagcg cgaagatcag
     2641 ctcgggttcg agcgagcctt cgatatggat atgcaactcg gccttcggtg cgcgagcggc
     2701 tttctctagc agtggggtca cggtcgttgt attcatggtc ggtcagaagt tcgttggagt
     2761 cggttcgcgc cggatgcgga gtgcgggacg ggacgcaacc gcgttagggc ggcaggtcga
     2821 ttgtgcggca atcagaccgt ttgcgccgcg tgcgcgcccg cattcgcctc cacggcctgc
     2881 aacacctggg cggccgccga gatcgcgatg atctccggcg acttgtcgac gataccgtcg
     2941 acgccgagcg ggcacttcat ccgcgcaatc tgcgacggat cgatgccgcg cgcggccagc
     3001 cggtgttcga actgcttgcg cttgctatgc gagccgatca tgccgaagaa cgcgaaatcg
     3061 ccgcgccgca gaatgcgctc ggccaactcg agatcgcgcg catggttgtg cgtcatcacg
     3121 atgaaatacg tgtgcggcgc ggcctgatcg atggcttcgt cgggcgcgtc gttcggatcg
     3181 atcttcacgt tcggcgcgtg cagcgtttcc accggtggaa actgcgcgtc gcgttcgtcg
     3241 acccagcgta ctgtgcacgg cagcgtggcg aatacgcgca ccagcgccgc gcccacgtgt
     3301 ccggcgccga acagcacgac ccggaagtcg cctggcgcga tggtttcggt gagcagcgcg
     3361 ccgctgtcgt cgaaaccggc gccgtcccac agcagacagt cggcggcatc gacacccggc
     3421 tcggggtcgg acagcatcac cgcatccggt gcggggccga atgatacgct acgcaccgtc
     3481 gactggcccg cggccacgcg tttcgccagc gtcgtgatcc agccgaggtc gcctacgtcg
     3541 agccgctcga acgccagaat caccgcgccg ccgcagcatt ggccgaggct cggaccgagc
     3601 gcgaagcgct ccagccgccg catgtgcggc gagcgcatgc cgtcgc
//
LOCUS       DATASH010000125         1686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4962309,
            whole genome shotgun sequence.
ACCESSION   DATASH010000125 DATASH010000000
VERSION     DATASH010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4962309"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="VHV99_03685"
     CDS             <1..436
                     /locus_tag="VHV99_03685"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX3634582.1"
                     /translation="SVTGIAGMNGKVLGLKLGSPGQILEPKLDAQLKAAGGAGFKDVK
                     TFDDHPAAYIALAEGKVDGVLNTLPTLALVLKNAPGKYAIVKGIGADNWAGIAARTDD
                     TALIGFIDQQLHQFKTDGTLHKLQQKWFGFDMNLPDAIPALT"
     gene            450..1097
                     /locus_tag="VHV99_03690"
     CDS             450..1097
                     /locus_tag="VHV99_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX3634583.1"
                     /translation="MDWSIIVMALPILAVGLLATLKVCLAAIAIGIVLGFALGFGALS
                     RFRAVRIAVLAYVDFVRGTPLLVQIFLVYFALPVIGINFNEYWAGVIALSLNAGGFIC
                     EIVRASMQSVDRGQTEAAQSIGMRHGQIVLHVLFPQAWRRILPPLTNELISLIKGSAL
                     LSAISVYELTHAGQEIIATYFSPFEIFLLIALYYYALISALAWLSRYLERRLPQY"
     gene            1120..>1686
                     /locus_tag="VHV99_03695"
     CDS             1120..>1686
                     /locus_tag="VHV99_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3634584.1"
                     /translation="MSSTVTVSDVPAAPAMDAAARVHAGGAATLLSIEGLHKRFGDKV
                     ILPSVSLQVEHGETVVLIGPSGSGKTTLLRCLNFLERPDGGTIRLGGDRIGLSEQGRT
                     LPEAELARQRSRIGFVFQRFNLFAHLSALDNVAAGPQRVLGLSRDAARARAQSALEKV
                     HLAQHLGKRPAQLSGGQQQRVAIARALAM"
BASE COUNT          282 a          553 c          515 g          336 t
ORIGIN      
        1 gagcgtcacg ggcattgccg gcatgaacgg caaggtgctg ggactcaagc tcggctcccc
       61 gggtcagata ctggagccca aactcgacgc gcaattgaaa gcagcgggcg gcgcgggctt
      121 caaggacgtg aaaaccttcg acgaccatcc ggccgcttat attgcactgg cagaaggcaa
      181 ggtcgatggt gtgctcaaca ctttgccgac gcttgcgctc gtcctcaaga atgcgcccgg
      241 caaatatgcc atcgtcaagg gcatcggcgc ggacaactgg gcgggcatcg cggcgcgcac
      301 cgatgacacc gcactgatcg gcttcatcga tcagcagttg catcagttca aaaccgacgg
      361 cacgctgcat aagctccagc agaagtggtt tggcttcgac atgaacttgc cggacgcgat
      421 tccggcgctt acctgagagc gcggcgtcca tggactggag cattatcgtc atggcgttgc
      481 cgatcctcgc ggtcggcctg ctcgccacgc tcaaggtctg cctcgcggcg atcgcgattg
      541 gcatcgtgct cggcttcgca ttgggctttg gcgcactcag ccgcttccgg gcggtccgca
      601 tcgccgtgct ggcctatgtc gatttcgtgc gcggcacacc gctgctggtg cagatctttc
      661 tcgtctactt cgcgctgccg gtcatcggca tcaacttcaa cgagtattgg gccggcgtca
      721 tcgcgctcag cttgaatgcg ggcggcttca tctgcgagat cgtgcgtgcc agcatgcagt
      781 ccgtcgatcg cggccagact gaggccgcgc aatccatcgg catgcggcac ggtcagatcg
      841 ttctgcacgt gctttttccg caagcgtggc gacgcatctt gccgccgctc accaacgaac
      901 tgatctcgct gatcaagggc tcggcgctgc tgtccgccat ctccgtgtat gaactcacgc
      961 acgccgggca ggaaatcatt gcgacgtatt tctcgccgtt cgagattttc ctgctgatcg
     1021 ccctctacta ctacgcgttg atctcggcgc tggcgtggct gtcgcgctac ctcgagcgac
     1081 gactgccgca atattgactt catgagttcg ggttcccgca tgtcttccac cgttaccgtc
     1141 tctgacgtac ccgcagcccc cgcgatggac gccgccgcgc gcgtgcatgc aggcggggct
     1201 gcaacgctgt tgtccataga aggcctgcac aagcgcttcg gcgacaaggt cattctgccc
     1261 tccgtctcgc tgcaggtcga acacggcgag accgtcgtgc tgatcgggcc gtcgggttcg
     1321 ggtaagacta ccctcctgcg ctgtctcaac ttcctggagc ggcctgacgg cggcacgatc
     1381 cgccttggcg gcgaccgtat cgggctgtcg gagcaaggcc gcacgctgcc cgaagccgaa
     1441 ctggcgcgac agcgcagccg catcggcttc gtgtttcagc gcttcaatct gttcgcgcat
     1501 ctgagcgcac tggataacgt cgccgccggc cctcagcgcg tgctggggct gtcgcgcgat
     1561 gcggcccgcg ctcgcgccca gtcggcattg gaaaaagtcc atctcgcgca acacctcggc
     1621 aaacggccgg cgcaactctc tggcggccag cagcaacgcg tggcgattgc ccgcgcgctc
     1681 gcgatg
//
LOCUS       DATASH010000126         2061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_610368,
            whole genome shotgun sequence.
ACCESSION   DATASH010000126 DATASH010000000
VERSION     DATASH010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610368"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1128)
                     /locus_tag="VHV99_03700"
     CDS             complement(<1..1128)
                     /locus_tag="VHV99_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX3634585.1"
                     /translation="MTSRPVIDDLSSFWMPFTANRQFKAAPRLLESAKGMYYRTTDGR
                     EVLDGCAGLWCVNAGHGRDEIVAAITQQLSTLDFAPTFQMGHPLAFEAATKVAELMPN
                     GLDRIFFTNSGSESVDTALKIALAYHRSRGEGQRTRLIGRERGYHGVGFGGISVGGIA
                     PNRKTFSGALLPAVDHLPHTHNLDHNAFTKGQPAWGAHLADELERVVTLHDASTIAAV
                     IVEPVAGSTGVLIPPQGYLQKLREICTKHGILLIFDEVITGFGRIGKATASEYFGVTP
                     DLITLAKAINNAAIPMGAVAASRAIHDSIVNAGAQGAIELFHGYTYSAHPAAAAAAVA
                     TLDLYRREGLFERAATMAPKFEAAAHSLKGAKHVKDVRNLGM"
     gene            1346..>2061
                     /locus_tag="VHV99_03705"
     CDS             1346..>2061
                     /locus_tag="VHV99_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018423551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX3634586.1"
                     /translation="MIELQLERDRRAAPTLVEQVVQGFADAIEAQSLRAGALLPSVRQ
                     LAQSHELSTFTVTEAYNRLVSMGLVVARRGSGYRVAARAQPARVAPTDWQPPSLTATW
                     LLSDVFADHSVPIKAGGGWLPNEWINETGLQHALRAMSRVPAARLGDYGHPYGFAPLR
                     ERIAEQLDRRGLPVDVSNVLLTQGATQGLDLIVRTLLRAGDVVIVEYPGYCNLLQILK
                     LAGLVVHGVPRTPAGVDTDVL"
BASE COUNT          326 a          650 c          702 g          383 t
ORIGIN      
        1 catgcccaga ttgcgcacgt ccttcacatg tttcgcgccc ttgagcgagt gagccgcggc
       61 ttcgaatttc ggcgccatcg ttgccgcgcg ctcgaacaga ccttcgcggc ggtacaggtc
      121 gagtgtcgcc accgcagccg ctgccgcggc cgggtgggcc gaatacgtgt agccgtggaa
      181 cagttcgatc gcgccttgcg cgccggcgtt gacgatcgag tcgtggatcg cgcggctcgc
      241 ggccaccgcg cccatcggga tcgcggcgtt gttgatcgcc ttggccagcg tgatcaggtc
      301 cggcgtcacg ccgaaatact cgctagccgt cgctttgccg atgcggccga aaccggtgat
      361 cacttcgtcg aaaatcaaca ggatgccgtg cttcgtgcaa atctcgcgca gcttctgcag
      421 atagccttgc ggtggaatca gtacgccggt cgagccggcc accggctcga cgatcaccgc
      481 ggcaatcgtc gaggcatcgt gcagcgtgac aacgcgctcg agttcgtcgg cgagatgcgc
      541 gccccaggcg ggctggccct tcgtgaacgc gttgtggtca aggttgtgcg tgtgcggcag
      601 atggtcgacc gccggcagca gtgcgccgga gaacgtcttg cggttcggcg cgataccgcc
      661 gaccgaaatg ccgccgaagc ccacgccgtg atagccgcgc tcgcggccga tcaaacgcgt
      721 gcgctggccc tcgccgcgcg agcggtggta cgccagcgcg attttgaggg cagtgtcgac
      781 tgattccgaa ccggagttcg tgaagaagat ccggtccagg ccgttcggca tcagttcggc
      841 aactttcgtg gccgcttcga atgccaacgg atggcccatc tggaaggtcg gcgcgaaatc
      901 gagcgtcgac agttgctggg tgatcgcggc gacgatttcg tcgcggccgt ggcccgcgtt
      961 cacacaccag agacccgcgc agccgtcgag cacttcacgc ccgtccgtgg tgcggtagta
     1021 catgcccttg gccgattcca gcaggcgcgg cgcggccttg aactggcggt tggcggtgaa
     1081 gggcatccag aaagacgaca ggtcgtcgat gacagggcgc gaagtcatgg ttttctcctc
     1141 gaagtggtag cgaaactgta gcgcccgcgg tttaatggcg gcataaacag ttgacgcagt
     1201 gtggcgctaa ctgtgttggt ggaatggcgg taactgtgtc gatcgcgccg tgacgtaatg
     1261 cggcgtttta tctggcgcct gcctcgcgct ctccacctgt ttggccactc agccggccgt
     1321 cgcgaccggc ctttcagccc agaccatgat cgaactacaa cttgagcgcg accggcgcgc
     1381 cgctcccacg ctggtcgagc aagtcgtgca gggtttcgct gacgcgatcg aggcgcaatc
     1441 cctgcgcgcc ggcgcgctgc tgccgtcggt gcgtcagttg gcgcaaagcc acgaactgag
     1501 caccttcacg gtcaccgagg cgtacaaccg tcttgtttcg atgggcctgg tggtggcgcg
     1561 gcgtggctct ggttatcgcg tggcggcacg cgcgcagccc gcgcgggtcg cgccgaccga
     1621 ctggcagcca cccagcctta ccgcgacctg gctgctgtcc gacgtgttcg ccgaccattc
     1681 ggtgccgatc aaggcgggcg gcggctggct gccgaacgag tggatcaacg agacgggttt
     1741 gcagcatgcg ctgcgtgcaa tgagccgggt gccggcggcg cgtctcggcg actacgggca
     1801 tccgtacggc tttgcgccgc tgcgcgagcg gatcgccgag caactcgacc ggcgcgggtt
     1861 accggtcgac gtctccaacg tgctgctcac gcagggcgcg acgcaagggc tcgatctgat
     1921 cgttcgcacg ttgctgcgcg cgggcgacgt cgtgatagtc gaatatcccg gttattgcaa
     1981 tctgctgcag attctcaaac tggcggggct ggtggtgcat ggcgtgccgc gcacgccggc
     2041 cggcgtcgat accgacgtgc t
//
LOCUS       DATASH010000127         5022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_17451,
            whole genome shotgun sequence.
ACCESSION   DATASH010000127 DATASH010000000
VERSION     DATASH010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5022
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17451"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..2272
                     /gene="fusA"
                     /locus_tag="VHV99_03710"
     CDS             167..2272
                     /gene="fusA"
                     /locus_tag="VHV99_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX3634587.1"
                     /translation="MARKTPIERYRNIGISAHIDAGKTTTTERILFYTGVTHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWKGMAGNYPEHRINIIDTPGHVDFTIEVERS
                     MRVLDGACMVYDSVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGADFFRVQRQIGD
                     RLKGVAVPIQIPVGAEDHFQGVVDLVKMKAIYWDEENQGIKFEYREIPAELADTAKEW
                     HDKMVEAAAEASEELLDKYLGGETLTEEEIKHGIRVRTIANEIVPMLCGSAFKNKGVQ
                     AMLDAVIDYLPSPLDIPAITGHDEHDREIERHPKDDDPFSALAFKIMTDPFVGQLIFF
                     RVYSGVVNSGDTVYNATKEKKERLGRILQMHANERKEIKEVYCGDIAAAVGLKEATTG
                     DTLCDPQHVIILEKMIFPEPVISQAVEPKTKVDQEKMGIALNRLAQEDPSFRVQTDEE
                     SGQTIISGMGELHLEILVDRMKREFGVEATVGKPQVAYRETVRNKIEDVEGKFVKQSG
                     GRGQYGHAVITLEPSAQGKGYEFVDAIKGGVIPREYIPAVDKGIQETLKAGVLAGYPV
                     VDVKVTLTFGSYHDVDSNENAFRMAGSMAFKDAMRKAKPVLLEPMMAVEVETPEDFMG
                     NVMGDLSSRRGMVQGMEDIAGGGGKLVRAEVPLAEMFGYSTSLRSATQGRATYTMEFK
                     HYAETPNNVAEAVINAKHK"
     gene            2512..3027
                     /locus_tag="VHV99_03715"
     CDS             2512..3027
                     /locus_tag="VHV99_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX3634588.1"
                     /translation="MSQDHEHPHYKDEQASAPIDWREHGVKVIKGDQLDSNTAQTPGM
                     NRAAAINAARVGAQKIWAGTVTIHPNAKTGAHHHGALESVIYVVRGQARMRWGEHLEF
                     TAEAGPGDFIFVPPYVPHQEINASTDDPLECVLVRSDNEAVVVNLNIDAVEAPETVFW
                     VDPIHKHPHDH"
     gene            3046..4065
                     /locus_tag="VHV99_03720"
     CDS             3046..4065
                     /locus_tag="VHV99_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HEX3634589.1"
                     /translation="MTPTASLRPRLITLYAVLIAANLGAWAWALIAFRHYPLLLGTAL
                     LAYGFGLRHAVDADHIAAIDAVTRKLMQTGKRPLGVGLAFSLGHSTIVIVATIGIAWT
                     AHSLHGRFETFKAVGGTIGTLVSAVFLLVLACVNLVILRDVWRRYRHAQHGGELAAET
                     TGAIAPAGLLSRALRPMFRLVTKSWHMYPVGVLFGLGFDTATEIGLLAIAASQASKGL
                     PLYSILVFPALFTAGMTLVDSTDNVLMVHAYGWAMDDPKRKLYYNASITFVSAVVAIV
                     IGGVEALGLLSDKLGLNGGAWDAMASLNERFGVLGYGIVAMFMACWIGSVLFHRWRRP
                     ATLAR"
     gene            4395..>5022
                     /locus_tag="VHV99_03725"
     CDS             4395..>5022
                     /locus_tag="VHV99_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3634590.1"
                     /translation="MKTPADRFSELELARHIADAYGNHAIDEFIAGRITRRDLLRYAS
                     VIGLSLVGGGVLNAPRARAQGAPSPANQTIRVAHLTPAGAVDPLTVTDAASLALLNQT
                     GEFLIDDDGEKLVLKPALALSWKPNDKGDVWTFKLRANVKFHDGQAFAAKDVVATFDR
                     LADPASGSAALSVLKGVLSKGGAKVVDDHTVAFHLDAPNGNFPYYVSSD"
BASE COUNT         1034 a         1576 c         1581 g          831 t
ORIGIN      
        1 gccggccagt cgcgaagcag cgacggccgc gcgtgcggac cggaccgctt ctcctcgagg
       61 gtcatcgagg taactgagga ggagcaccga caccgcgagc ctgcgatggc ccgaaccgaa
      121 gacaacggga ccgcagcagg agcaggagat tagtcaggga tttgatatgg cacgcaagac
      181 cccaatcgag cgttaccgga acatcggcat cagcgctcac atcgatgccg gcaaaaccac
      241 caccaccgaa cgcatcctgt tctacaccgg cgtgacccac aagatcggtg aggttcacga
      301 cggtgcggcg acgatggact ggatggaaca ggagcaggag cgcggcatca ccatcacgtc
      361 ggcggccacc actgccttct ggaaaggcat ggccggcaac tatcccgaac accggatcaa
      421 catcatcgac accccgggtc acgtcgactt cacgattgaa gtggaacgtt cgatgcgcgt
      481 gctcgacggc gcatgcatgg tgtacgactc ggtgggcggc gtgcagcctc agtccgaaac
      541 cgtctggcgt caggcgaaca agtacaaggt gccgcgcatt gcgttcgtca acaagatgga
      601 ccgtgtgggc gcggacttct tccgcgtgca gcggcagatc ggcgatcgcc tgaagggcgt
      661 cgcggtaccg atccagattc cggtcggcgc ggaagatcat ttccagggcg tggtcgacct
      721 cgtcaagatg aaggcgatct actgggacga agagaaccag ggcatcaagt tcgagtaccg
      781 cgagatcccg gcggaactcg cggataccgc gaaggaatgg catgacaaga tggtcgaggc
      841 cgccgctgaa gcaagcgagg aattgctcga caaatacctg ggcggcgaga cgctgactga
      901 agaggaaatc aagcacggca ttcgtgtgcg taccatcgcc aacgagatcg tgccgatgct
      961 gtgcggcagc gcgttcaaga acaagggcgt gcaggccatg ctcgacgcgg tgatcgacta
     1021 tctgccttcg ccgctcgaca ttccggcgat caccggccac gacgagcacg accgcgagat
     1081 cgagcgtcat ccgaaagatg acgatccgtt ctccgcgctc gccttcaaga tcatgaccga
     1141 cccgttcgtc ggccagttga tcttcttccg cgtgtattcg ggcgtggtga attcgggcga
     1201 cactgtctac aacgcgacca aggaaaagaa ggaacgcctt ggccggatcc tgcagatgca
     1261 tgcgaacgaa cgcaaggaaa tcaaggaagt ctactgcggc gacattgccg cggcagtcgg
     1321 actgaaagaa gcgaccacgg gcgacacgct gtgcgatccg caacatgtga tcatccttga
     1381 aaagatgatc ttcccggagc cggtgatttc gcaggccgtc gagccgaaga ccaaggtcga
     1441 ccaggagaag atgggcatcg cgctcaatcg tctcgcgcag gaagacccgt cgttccgcgt
     1501 gcagacggat gaagaatccg gccagaccat catctccggg atgggcgagt tgcacctgga
     1561 aattctggtc gaccggatga agcgcgagtt cggcgtcgag gcgaccgtcg gcaagccgca
     1621 agtggcttac cgcgaaacgg tgcgcaacaa gatcgaggat gtcgaaggca agttcgtcaa
     1681 acagtcgggc ggccgcggcc agtacggcca cgcggtgatc acgctcgaac cgtcggcaca
     1741 aggcaagggc tacgagttcg tcgacgcgat caaaggcggc gtgattccgc gcgaatacat
     1801 tccggcggtc gacaaaggca ttcaggaaac gctgaaggcc ggcgtgctgg cgggctatcc
     1861 ggtggtcgac gtgaaggtga cgctgacctt cggttcgtac cacgacgtcg actcgaacga
     1921 aaacgcgttc cgcatggccg gctcgatggc tttcaaggac gcgatgcgca aagcgaagcc
     1981 ggtgctgctc gaaccgatga tggccgtcga agtggaaacg ccggaagact tcatgggcaa
     2041 cgtgatgggc gatctgtcga gccggcgcgg catggtgcaa ggcatggaag acatcgccgg
     2101 tggcggcggc aagctggtgc gcgccgaagt gccgctcgcg gagatgttcg gctattcgac
     2161 gtcgctgcgt tcggctaccc aaggccgcgc gacctacacg atggagttca agcactacgc
     2221 tgaaacgccg aacaacgtcg cggaagccgt gatcaacgcg aagcacaagt aagcggcaag
     2281 ggtggcaacg cgctatgtgc tgcgaccctc ggcgtaacga acgcagcgaa cgcaacgaac
     2341 acagcgaaag cagcgaaagc aagcagcaaa aagcccgtcc cccgtggatg ggcttttttt
     2401 cgtccgcgac gccgcctggg gccgccggca acacacacgt ttttgaacgt gcaaaaatgc
     2461 cagacggcgc cgtgccgacc ggccggactc acgcccacga ggagacacac catgagccag
     2521 gatcacgaac acccgcatta caaggatgag caagcatcgg cgccaatcga ctggcgcgag
     2581 cacggcgtca aggtcatcaa gggcgaccag ctcgatagca ataccgcgca aacgcccggc
     2641 atgaatcgcg cggcggcgat caacgcggcg cgcgtcggtg cgcagaaaat ctgggccggc
     2701 accgtgacga tccatccgaa tgcaaagacc ggcgcgcatc atcacggcgc gctcgaaagc
     2761 gtgatctacg tggtacgtgg ccaggcacgc atgcgctggg gcgagcatct ggaattcacg
     2821 gccgaagccg gtccgggcga cttcatcttc gtgccgcctt atgtgccgca tcaggaaatc
     2881 aacgcgagca ccgacgatcc gctcgaatgc gtgctggtgc gcagcgacaa cgaagcggtg
     2941 gtggtgaacc tgaacatcga cgcagtggaa gcaccggaaa ccgtcttctg ggtcgatccg
     3001 attcacaaac atccgcacga tcactaaacc tctttcacgt aactcatgac gccgaccgcc
     3061 tcactgcgcc ctcgcctcat cacgctgtat gccgtactga tcgccgccaa tctcggcgca
     3121 tgggcgtggg cgctgatcgc gtttcgccac tacccattgc tgctcggcac cgcgctgctc
     3181 gcgtatggct tcggcctgcg ccacgcggtg gacgcggacc acatcgccgc gatcgacgcc
     3241 gtcacgcgca agctgatgca gaccggcaag cggccgctcg gcgtgggcct ggcgttttcg
     3301 ctgggccatt cgacgattgt gatcgtggcg acgatcggca ttgcctggac cgcgcattcg
     3361 ctgcacggcc gcttcgaaac cttcaaggcg gtgggcggca cgatcggcac actcgtgtcg
     3421 gcggtgtttc tgcttgtgct ggcgtgcgtg aatctcgtga ttctgcgcga cgtctggcgc
     3481 cgctatcgcc atgcccaaca tggcggtgaa ctcgcggccg aaacgacggg cgccatcgcg
     3541 cccgccggtc tgctgtcgcg cgcactgcgg ccgatgttcc ggctcgtgac gaaaagctgg
     3601 cacatgtacc cggtcggcgt gctgttcggc ctcggcttcg acaccgcgac cgagatcggc
     3661 ctgctggcaa ttgcggcgtc ccaagccagc aaaggtctgc cgctgtattc gatcctcgtg
     3721 ttcccggcgc tcttcaccgc cggcatgacg ctggtcgatt cgaccgacaa cgtgctgatg
     3781 gtccacgcct acggctgggc catggacgat ccgaagcgca agctctacta caacgcgagc
     3841 atcacgttcg tctcggcggt ggtggcgatc gtcatcggcg gcgtcgaagc gctgggattg
     3901 ctatcggaca agctcggttt gaatggcggc gcatgggacg ccatggcaag cctcaacgaa
     3961 cgcttcggcg tgctgggtta tggcatcgtc gccatgttca tggcgtgctg gatcggctcc
     4021 gtgctgttcc accgctggcg ccgcccggcc accctcgcgc ggtaaatcca acttgccctg
     4081 cgcgccagat cggtaacgtt tccacggcgg tagcgacgat ttccgcgcta ccgcatccgg
     4141 cgatgtcgcg caggccatgc gagccagccc gtcgagcgtt gtcgtgacga cacacgcggc
     4201 cccttttatc cgccaaacgt gaaccggacc gcgcagcagg cgcgccgtac ccgggagcaa
     4261 tcgcttacac tgaaaccgct ttttgccact gcggctgctc agcagcttgt cgtcattgca
     4321 atcaagtcgc cctgtcgcgt gcatctcgcg cggcgcttca gaaccggcaa cagaacggac
     4381 tggacactcc atcgatgaaa acacccgcgg accgattttc cgaactcgag ctggcccgcc
     4441 acatcgccga cgcctacggc aaccacgcca tcgacgaatt catcgccggg cgcatcacgc
     4501 gtcgcgatct gctgcgctat gcaagcgtga tcgggttgtc gctggtcggc ggcggcgttc
     4561 tgaatgcgcc gcgggcccgt gcgcaaggcg cgccgagtcc ggctaaccag acgatccgcg
     4621 tagcccatct gacgccggcc ggcgcggtcg acccgctcac cgtgaccgat gccgcgagcc
     4681 tcgcgctgct caaccagact ggcgagttcc tgatcgacga cgacggcgaa aagctcgtgc
     4741 tgaaaccggc gcttgccttg tcatggaagc cgaatgacaa gggcgacgtg tggaccttca
     4801 aactgcgcgc caacgtcaag ttccacgacg gccaggcatt cgccgccaag gacgtggtcg
     4861 ccaccttcga tcgcctcgcc gacccggcga gcggttcggc ggcgctctcg gtgctcaagg
     4921 gcgtgttgtc gaaaggcggc gcgaaggtgg tcgacgacca cacggtcgcc ttccatctcg
     4981 atgcaccgaa cggcaacttc ccgtactacg tttcctcgga ca
//
LOCUS       DATASH010000128        21738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4962837,
            whole genome shotgun sequence.
ACCESSION   DATASH010000128 DATASH010000000
VERSION     DATASH010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 21738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21738
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4962837"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VHV99_03730"
     CDS             <1..215
                     /locus_tag="VHV99_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEX3634591.1"
                     /translation="VGDITGLMPGPDDMELTPDKRYLWVGFRFAKHVGIIDLTTRKLI
                     ETIAVGRSPHGLFFDNRAPVVAPNPD"
     gene            285..1292
                     /locus_tag="VHV99_03735"
     CDS             285..1292
                     /locus_tag="VHV99_03735"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016344908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX3634592.1"
                     /translation="MFHEIAAQLDNMVSTLQMLLYVDVVQPFFFRFGLMGYDEDTYDA
                     LYWVIIGVLEIVVMYLALRPLEALRPIEQWQDRKALRADVIYTWIAKLGIINVAFFFI
                     LQPLFFHWQSLISIYGIPNTDIDSLWPGVTDQPVVSFLIYLILLDFAGYWYHRWQHRF
                     GVWWELHAVHHSQQQMSLWADDRNHFLDDMIQAAFFAAISLFIGVQPSQFVVLVAVSN
                     SLQSIQHVNARLPYGWLLERIIVSPIFHRRHHAVGYGHEGTAYGCNFGVLFPWWDMMF
                     RTVSWNPAVEVTGIRDQLPVPHGSGRSYGDGLIAQQWLTFGRIVKRLRGQRNYSGGAA
                     E"
     gene            complement(1350..2279)
                     /locus_tag="VHV99_03740"
     CDS             complement(1350..2279)
                     /locus_tag="VHV99_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3634593.1"
                     /translation="MRFKLRQMEVFRAVMLTGSINAAARMLYVSQPAVSKLVSHTETT
                     LGLRLFERSKGRLIPTAEAQALFREVEQVYQSALRVDEFARALALGPASLLRISCSPS
                     LGPTIVAPAIVELKRSMPKLRIDWHTTLMSDMPLEVLSKAVDIAVTSMPIEHEHLEVV
                     PFMRGRMVCALPADHPLAAKERIALADLKTEPMILFRRDIPFGTVIVRACQQADVELT
                     SVVDVTRADQALALVQGGLGLTIVDEFAAGSVDCAVRPLVEELEMISTFVYSKFSPPP
                     RGAMLLMEAVYHRAEQLGRQVGNLRATGCASRE"
     gene            2427..2615
                     /locus_tag="VHV99_03745"
     CDS             2427..2615
                     /locus_tag="VHV99_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634594.1"
                     /translation="MNPTYPPRALDTQAAAYASSPVHTRARFGILALLPIGTTIHYLH
                     RSVAGIAAPAMRSRSSRP"
     gene            2885..3760
                     /locus_tag="VHV99_03750"
     CDS             2885..3760
                     /locus_tag="VHV99_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009771517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3634595.1"
                     /translation="MSVFDEARIDCHCHAFDPIRFPYRDDTAYRPAQQEVGTPAQLIR
                     VMDAYGVRHALLVGPTSGYRTDNRCLLDALETHKARFRGIAVVDNDIGRDELAALRQA
                     GVVGVAFNPAMEGIELVLSAGALFAALADLDMVAQLQVAGDQMVSLGPWLARQPATLL
                     VDHCGRPDIAAGVGQAGFQALLRLADTGRTTVKLSGWQKYSRVPHPYEDAWPYAHALL
                     RTFGPQHCVWGSDWPFLRAPERLDYGPLLTLFGQMVPDVAVRRRIQWDTPRRLFGFDA
                     FPDTADTTHSESVAS"
     gene            3784..4794
                     /locus_tag="VHV99_03755"
     CDS             3784..4794
                     /locus_tag="VHV99_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008354231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3634596.1"
                     /translation="MDKMKPLRFGVLGAAKIARSFIAGVGPSEIVDIVAVASRDLAKG
                     KQFANELGVPHAHGSYEALIDDPNVDAIYVPLPNTLHAEWVIKALNAGKHVLCEKPIA
                     ISQTDTLAMFEAARRNRRFLAEAYPYRAQEQTLALRRLLDEGAIGRVQTVFACFGVKF
                     ADPSNIRLDPERGGGALLDAGSYATNMVRMVAGEKPSRVNAIARWAQTQVDLTVIANL
                     EFPSGLLGQISCSFNTAYHRHALIAGTDGILETTFLNSPPDAGPPEIHVRRGIPATTV
                     RETITLAGGSGFLAEAESFARAVALGSEHWTGSTEQESIETIATLEAIARSIRSGNWE
                     NV"
     gene            4887..6233
                     /locus_tag="VHV99_03760"
     CDS             4887..6233
                     /locus_tag="VHV99_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3634597.1"
                     /translation="MKTDATLATAGDLSLASTRRSRTRFTILALLAIGTVINYLDRTV
                     LGIAAPSLTKDLGLSAGVMGIVFSAFSWTYTLSQIPAGLLLDRLGARKTYFLAVASWS
                     LFIMLNGLSGGLVSLLFCRLGLGVAESPCFSTNSRVVGHWFPQRERARATSVYQVGQY
                     IGLAVFSPLLFWIIAAFGWRAMFVIVGAVGLAFALVWWLRYHEPHESRRMNDAERDHI
                     GAGGGLATAADAQRPFSWQDVRSLLGRREIWGAAIGQFAGNSTVVFFLTWFPTYLVTE
                     RHMAWLKIGFFAILPFLGASVGVLFGGWLSDFLLRRTGRINLARKLPIITGLLLSSSI
                     VAAIYVKSDAAVIAVLSLAFFGQGMGGLGWSIIPDIAPKRLMGLTGGIFNFTANIAGI
                     VTPIVIGTIVALTGSFYWGLMFIGAVAVLGAASYIFILGDIKRIELPEARVAGRMS"
     gene            6291..7166
                     /locus_tag="VHV99_03765"
     CDS             6291..7166
                     /locus_tag="VHV99_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HEX3634598.1"
                     /translation="MRHSNYACEDIGNLVLLEHVNLTIPDQRLATAFYVSALGLTRDP
                     FLMTGVTNMWINAGRSQIHLPHGDAQCLRGHVGLIVGRRAPLIERLRAVGPLLEQTRF
                     GWTERDDRVEVICPWGNRYDCLDPDACAASDPWAGIDLGIVYVQLDVPVHCARSIAAF
                     YREIFDASVDLRERNGSRQAVVSVGRHQQLVYAETPSASQEYDGHHIQIYVANFSGPY
                     ERLADRGLNYGEEPHQYRFAELVEPVSGTHCFTLEHEVRSLRHPLYARPLVNRNPDVT
                     NRNYRMGGEGFGGVF"
     gene            complement(7179..8093)
                     /locus_tag="VHV99_03770"
     CDS             complement(7179..8093)
                     /locus_tag="VHV99_03770"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3634599.1"
                     /translation="MVMNLQMRDLLYFAKVAELGHLGRASQALHITQPALSKCIDRLE
                     ETYGADLFERRGRGIRLTDAGQLLYERSLLIERTLDETHRQISSLGKGLAGLVRVGAA
                     ATVAEFVMPTVCRLLQQEAPAVVIELQIGMNDVLHESLRKRLLDVVVGPLSTHDEELV
                     AIPISTDQVVVAVSATHPLAGKHASLEDMCGYGWVLPAQSVATRQWLERVFASRGLPL
                     PRAAVMTSSIAAVPRLIAETGLLSFISRRNLNEARFTPRLVEVFNPDTTMTRNFGVVH
                     RTDPYLSPATQRFIQIVQAQSEAPRHDI"
     gene            8225..9217
                     /locus_tag="VHV99_03775"
     CDS             8225..9217
                     /locus_tag="VHV99_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEX3634600.1"
                     /translation="MSYRILVTAPRLAAAGQRALSDAQCEVTYIGNAQDAAEVERVLT
                     TQPIDAVISRTVDLSASAIEGCPTLKVICKHGVGVTNIDVDAATRAGIPVFTTPGTNT
                     LSVAELTIALMLSAARKVNFYDSELRAGRWTRTGDGIQLHDRVLGLVGYGQIGRNVAK
                     IAHALGMRVHVFDPALRTGGVESYVTVERSPQELYARADVLSLHCPVNTHKRGMIDAH
                     AIAALPDRAIVINTARGELIDEAALVAALQSGKLAAAGLDTFAHEPLGGEDPLLALPN
                     VVLTPHVGGSTPDALDAVATSAVATCLRYLRGDAVDSQRCVNPHVLLSDKKVIA"
     gene            9214..9915
                     /locus_tag="VHV99_03780"
     CDS             9214..9915
                     /locus_tag="VHV99_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEX3634601.1"
                     /translation="MSDIQDRAGWPAGFFVGRRTAVPSAAQVDAFRKVPVAHAGDCMG
                     RSIGATGLLAYHNSLSLTMCGPAITVRVRPGDNMMIHKAIELAQPGDVIVVDGGGDLT
                     QALIGGLMRTSALAKKIGGFVIDGAVRDLAEWAEGVIPVYARGHTFRGPSKEGPGEVN
                     VPIACAGMAVNAGDLMLGDADGVIAVPVDQLDALLPLVLAHATKEEGIRAANRAGSAD
                     PERFNALLRKKGCPV"
     gene            10005..11501
                     /locus_tag="VHV99_03785"
     CDS             10005..11501
                     /locus_tag="VHV99_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008353776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3634602.1"
                     /translation="MHDYSNVAAPGGNGGTPSTLHISSAPRVTFTPEELVRHMSARLD
                     RLPASRPVWLLIGALALGGWFEIYDMFLTAYIGPGLVKAGIFTKTTASLFDLGGLGAF
                     VAALFLGLFIGTIGVAFIADRFGRRKVFVGALLCYTFAAVMMSVQHDPVWIVFWRMMA
                     GIGVGAELVTIDAYVSEFVPASIRGRAFAFVQAIQYTSIPTIAFLSWQLVPIAPFGHD
                     GWRWVVWIGCSGAVFVWLIRLGLPESPRWLIQQGRAAEAETIIARLERRIEQATGRAL
                     PPPSSLSDTANVAPARVATPSASEDGPWSQRYRNRTVMLLVFNFFQSIGYYGFASWVP
                     TLLAEKGVAFTHSLLYSFIIAIASPLGPLVAMLFADKLERKWLIAGSAATLAVLGVIF
                     AQQRDPAILVAMGLLMTLAGNCMTFSYRTYQAELFPTRMRARAIGLVYSASRVSAMLS
                     GFLIGFSLRHTGVAGVFALIAGSMFVVVLTIGFFGPRSRGLSLEQVSA"
     gene            11510..12436
                     /locus_tag="VHV99_03790"
     CDS             11510..12436
                     /locus_tag="VHV99_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX3634603.1"
                     /translation="MFYLNEPPALIETRVFSSMPAAFRNTGEVPAWAVANKAGQPVNS
                     FIEGPAFDAEGRLYIVDIPYGRIFRIGADGEWTLIAQYDGEPNGLKFHPDGRILVADY
                     RNGLMQLDPHNGRVSAFLTSRNGESFKGVNDLAIASNGDVYFTDQGQTGLHDPRGRVF
                     RLSATGRLDCLLDNGVSPNGLVLDAAERVLFVAMTRDNAIWRVPLLPDGGTAKVGRFC
                     TMFGTSGPDGLAMDEAGNLYVAHASLAHVLVFAPNGEWIARIKSCTGTTVTNVAFGGP
                     GRRQLFITESLTGTVLVADVPQAGIALPRKAA"
     gene            12798..14609
                     /gene="araD"
                     /locus_tag="VHV99_03795"
     CDS             12798..14609
                     /gene="araD"
                     /locus_tag="VHV99_03795"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007744097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinonate dehydratase"
                     /protein_id="HEX3634604.1"
                     /translation="METSVSSNRKTSEELRSYRWYGVNDLRSFGHRSRTAQMGYHASD
                     YMGKPVIAVVNTWSEINSCHTHFKQRVEEVKRGVWQAGGFPVEMPVMTLAEPFQKPTT
                     MLYRNFLAMETEELLKSYPFDGCVLMGVCDKTTPGLLMGAISMNLPAIFLPAGPMLRG
                     NWNGRTLGSGSDTWKYWAELRAGKITEEEWKGIESGIARSPGHCMTMGTASTMTSAAE
                     ALGLTLPGFSSIPAVDSRHAQFASLTGQRIVKMVWTDQKPSDILTAKSFDNAVTTVLA
                     MSGSTNAIVHLVAVARRAGIDLTTARFDELSRITPVIGNLRPSGQYLMEDFFYAGGLR
                     ALLVELGELIDGSQMTVNGSTLGENIAGAEIFDDDVIRKRSNPVVASDGLAVLTGNLA
                     PDGAVIKPAAMEAHLQKHRGPAVVFKDYADMAARIDSEELDVTADSVIVLQHAGPVGA
                     PGMPEWGQLPIPQKLLKQGVRDMLRISDARMSGTSYGACVLHVAPESFVGGPLALVKD
                     GDMIELDVAARRLHLEVSDAELAARKAAWQPPKLPFKRGFGVMHQLHVTQANKGCDFD
                     FLEEPVSETAAQAVQSAPTGQDTQDTPVAKRTEPEIH"
     gene            14626..15360
                     /locus_tag="VHV99_03800"
     CDS             14626..15360
                     /locus_tag="VHV99_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease activity regulator RraA"
                     /protein_id="HEX3634605.1"
                     /translation="MTASDISISEATLEQLRHVSTATLTTQLFKRGLRNVFLQGVAPL
                     VKPAPGAPNLVGPAFTLRNIPAREDLDHLGVFQDPDHPQRKAVETAPPGSVLVQDCRG
                     ERSVASVGSILALRLAKRGVAGMVSDGPVRDSGTISGLGLPLWCAGASAPLNLAKHHA
                     VDMNVPIACGGVPVYPGDIVVADVDGVVIVPREMAADVARDATEQEQLEVFITQRIDE
                     GRPLRGTYPPSEETMAAYAQWRAQQK"
     gene            15430..16755
                     /locus_tag="VHV99_03805"
     CDS             15430..16755
                     /locus_tag="VHV99_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3634606.1"
                     /translation="MTITTPAIGESRLINRLALRLMPLLGLLYLVAYIDRSNISFAKL
                     QILGSLGLSEVAYGLGASLFFIGYLIFEVPSNVLLHKYGAPRWMARIMFTWGIVTLLL
                     AFTQNATMFYILRFLLGASEAGLYPGVIYYLTLWFPSRHRVRMLGYFTVGSSLGNMVG
                     APICGWLLDKGGIAGLHGWQLVFIVTGLPSILLTFVVLYLLPASPKQATFLSADEKQW
                     LADTLDAERAQIRSGAGAHASLLSVLTEPRVIGMALYYMMLSISVYGVSYWLPTLVKG
                     FGVSNTTNGLLNIIPWLMATIVLAWLPSKLRGGNRALVAMLVAALLGVVCFLSAVFLP
                     TNTLRFAALCFGAPCMYLLIPCFWTLPPKFLFGARAAAGIAAINSLGNIGGFIAQNLV
                     PWVRQTTGSVKTPMLIPAACLLLFAGVTLLILRRDKGVRVAQRPVPVTD"
     gene            16809..16931
                     /locus_tag="VHV99_03810"
                     /pseudo
     CDS             16809..16931
                     /locus_tag="VHV99_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007733100.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            17061..18125
                     /locus_tag="VHV99_03815"
     CDS             17061..18125
                     /locus_tag="VHV99_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013341456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX3634607.1"
                     /translation="MPNHKPNAASPNAPKADAPAKRVRGGPKGLRIADVAAQAGVAPM
                     TVSRVLNSPETVAPETLERVRQVVQKLGYVPNRLAGGLSSSRSRLIAAIVPTIAHSLF
                     SETIQVFSETMSRAGYQVLLALSGYSDSSEEALLDAVLSRRPEGVLLTGVAHTESLRE
                     RLRNVGMPIVETWDMTGTPIDMLVGFSHYEIGAAVAEHFLRRKVRAPGLISANDDRAL
                     ARRDGFRERLTSAGIHEIAEVLVSPPSSVALGKAALPTLLEQAPELDAVFCGSDLLAI
                     GVLGAARRLGLDVPSQLSICGFGDLEFATETTPQLTTVLVDGTQIGLTAARCLLDRLA
                     GDAKTKVTDVGFHIVERETT"
     gene            complement(18165..19331)
                     /locus_tag="VHV99_03820"
     CDS             complement(18165..19331)
                     /locus_tag="VHV99_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009771455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase family protein"
                     /protein_id="HEX3634608.1"
                     /translation="MNSWLTQEAACEVLGVRKQTLYAYVSRGQIEVRRDPDHASRKLY
                     RESDVAALLKKRSLGRARKNIAASTMAWGEPIINTRISTIVRGRLYYRGKNAMELASC
                     LTLEEVAQLLWDSAEPLRFPVSEPVLNAGPLRARAYAAMGLAAAEGDSARTLDVDSMR
                     QEAQKLVGRLASSFVTLASTEEPLHLRIARAWRCERYADLLRRALVLLADQELTSSTF
                     AARVAASTGASLGACALAGLATLSGPLHGDATVRVQALTDDARQFGVEAAVKRWRSAG
                     KALPGFGHELYPQGDPRAVNLLGAFEPSEDTRKLIDHIGRETGLKPSIDIALTALAEH
                     CHLPEGAVFSIFAIARSVGWMAHSIEQSASRGLLRPRANYIGPPITGEGKSEED"
     gene            19566..20873
                     /locus_tag="VHV99_03825"
     CDS             19566..20873
                     /locus_tag="VHV99_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009771454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3634609.1"
                     /translation="MTHTRFALLATILLVATVAYADRAILSIAGPGIAKEFGLNHIQL
                     GYILSAFSWAYVIGQIPGGLLLDRLGTKAVYGATLVLWSIATILVGFIGKITSDLSVA
                     LGLLFALRFALGFIEAPSFPANSRVTVMWFPKDERGLATSLFASASYFAVAIFSPLAG
                     WLVAKFGWPAPFFALGLIGIASAGLWAVVMHEPRKHPRASQAELDHIVAGGAMVDIDS
                     SQERLSRPVVSSKVLRVLLGNRMLWCSYIGQYCAIALSYFFITWFPIYLVQARGMNIM
                     QAGLATMLPAISGFLGGIAGGVISDLLIRRGWSVSWARKTPYIVGMAVGCCLVFSAFT
                     ESNTVIVLLMTIAFFGKGTAAGAGTWAIVSDTAPREAVGLAGAIFNCVGNIGGIVTPI
                     VFGYIVEATGDYTAGLYFVAAHCLVAAVVYLFFMGKIERVKMS"
     gene            20953..>21738
                     /locus_tag="VHV99_03830"
     CDS             20953..>21738
                     /locus_tag="VHV99_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009771453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEX3634610.1"
                     /translation="MEKPIILKAAELPDWTTAELNALFTVLELPEGPAAASAFLKERG
                     PAIRGIALRKTKVDAAFLDAVPALEIISSYSAGLDNVDVQAVKARGIVIENSSHVLAE
                     DVANTALGLALAVTRDFVKADAYVRAGKWPEHGQFPLGRSISRMNVGIVGLGMIGSAI
                     ARRLQAFGSRVAYFGPTRKPVDIPYYDSVVGLAAACDMLILACPLSRATHHLVDATVI
                     DALGPGGFLVNISRGPVVDEAELIAALGADRIAGAALDVFEREP"
BASE COUNT         3862 a         6596 c         6872 g         4408 t
ORIGIN      
        1 atgtcggcga tatcaccggt ttgatgcccg gacccgatga catggaacta acgcccgaca
       61 agcgctatct atgggtggga ttccgcttcg cgaagcatgt gggcatcatt gatctgacca
      121 ctcgcaagct gatcgagact attgcggtgg gccgctcgcc gcacggcctc tttttcgaca
      181 accgcgcgcc ggtcgtggcg cctaatccag actagacggg gcaagttctc tgaaacttgc
      241 cgttatatga gctctggcag aactgctaaa acacgaggct tctgatgttt catgaaatcg
      301 ccgcacaact cgacaatatg gtgtctacgc tgcagatgct gttatatgtc gacgttgtgc
      361 agccgttctt ttttcgcttc ggcttgatgg gttacgacga agacacctat gacgcactgt
      421 actgggtgat catcggcgtg ctggaaatcg tcgtgatgta tctcgcgtta cgaccgcttg
      481 aagcgctgcg tcccatcgag cagtggcagg atcgcaaggc actgcgcgcg gacgtgatct
      541 acacatggat cgcaaagctg ggcatcatca acgttgcgtt ctttttcatc ttgcaaccgc
      601 tattctttca ctggcagagc ctgatatcga tctacggcat accgaacacc gatatcgaca
      661 gcctctggcc gggcgtgacg gatcagccag tcgtgtcgtt cctgatctac ctgatcctgc
      721 tcgattttgc cggctactgg tatcaccgct ggcagcatcg tttcggcgtg tggtgggaac
      781 tgcatgcggt gcatcacagc cagcagcaga tgtcgctgtg ggccgacgac cgcaatcact
      841 ttctcgacga catgatccag gcagcgttct tcgcggcgat ctcgctgttt atcggcgtgc
      901 agccgagcca gttcgtggtg ctggttgcgg tcagcaactc cctgcagagc atccagcacg
      961 tcaatgcacg cctgccgtat ggctggctgc tcgagcgcat catcgtcagc ccgatctttc
     1021 accgccgcca ccacgcggtg ggctacggcc atgaaggcac ggcttacggc tgcaatttcg
     1081 gcgtgctgtt tccatggtgg gacatgatgt tccgcaccgt ttcatggaac cccgccgtcg
     1141 aggtgaccgg catccgcgac cagctgcctg tgccgcatgg ttcaggccgt tcgtatggcg
     1201 acggattgat cgcgcagcag tggctcacgt ttggccgaat tgtgaagcgt ctgcgcgggc
     1261 aaaggaatta ctcgggtggc gccgcggagt gagtgcagct ggttgatcgt cctgcgaatg
     1321 cgcgagcggc tagcccggcg gtgccgggct cattctcgac tcgcgcagcc ggtagcgcgc
     1381 aagttgccaa cctgccgccc caattgttcg gcgcggtgat agacggcctc catcaacagc
     1441 atcgcgcctc tgggcggcgg tgagaatttc gaatacacga acgtcgaaat catttcgagt
     1501 tcctcgacga gtgggcgcac ggcgcaatcg acgctgcccg ccgcgaactc gtcgacgata
     1561 gtcagtccca gtccgccttg taccagcgcc agagcctgat cggcgcgggt tacgtcgacg
     1621 accgaggtga gttcaacatc ggcctgctgg cacgccctga cgataaccgt gccaaacgga
     1681 atgtcgcgcc tgaaaagaat catcggttcg gtcttgagat cggcgagtgc gatgcgctcc
     1741 ttcgccgcaa ggggatgatc tgccggcagc gcgcacacca tgcggccccg catgaacggc
     1801 acgacttcaa gatgttcgtg ctcgatcggc atcgacgtca ccgcgatgtc gaccgccttg
     1861 cttagcacct ctagcggcat gtcggacatc agcgtcgtat gccagtcgat cctgagcttc
     1921 ggcatgctgc gtttcaattc gacaatcgcc ggtgcgacga tcgtcggccc cagcgaaggg
     1981 ctgcaggaga tacgcagcag gcttgccgga ccgagtgcga gcgcccgtgc gaattcgtcg
     2041 acgcgcaacg ccgactgata gacctgctcg acctcacgaa acagggcttg ggcctcagcc
     2101 gttggaatca gccgaccctt cgaccgttcg aacagacgca ggccaagcgt ggtttccgta
     2161 tgcgagacga gcttgctgac ggccggttgc gagacatata gcatcctcgc cgccgcgttg
     2221 atcgagcccg tcaacatcac cgcgcgaaac acttccattt gccgcagctt gaagcgcatt
     2281 catccctccg aagcccgcgt cgggtgatta accaaaagtt ataggatagc tgaaacttgg
     2341 catacgacaa cggcgcgatt actccctagt atctccatga acgcagggcg atcgcctcac
     2401 agcgcccgac aaaacatgga gacagcatga acccgaccta tccgccgcgc gcgctcgaca
     2461 cacaggccgc cgcgtacgca tcgtctcccg tgcatacgcg ggcgcgcttc ggcatcctcg
     2521 cgttgctccc gattggtacg acgatccatt acctccaccg cagcgtcgcg ggcattgcgg
     2581 cgcctgcgat gcgctcgcgt tcatcccggc cgtgacgctg gctggggcgc tctcatacgt
     2641 attcgtcgtc ggcgacatca ggcggagcgt tctgcccgcc ggccgttctt ccacccattg
     2701 aatccactga gttacaaaag ggaagctttg ccatgaacca cacactcgac ggccaggtcg
     2761 cggccgtcac gggcgcggca cgaggcatcg gccttggtat gcagctttac gacgggcttc
     2821 gcgttcgacc tgacgggcgg gagggccacg tattgaaacc cgccacgaac cgcacggaga
     2881 cgccatgagc gtattcgacg aagccaggat cgattgccat tgtcatgcgt tcgatcccat
     2941 tcgctttccg tatcgcgacg acaccgcgta tcggcccgcg caacaggaag tcggcacgcc
     3001 cgcgcagttg atccgcgtca tggacgcgta tggcgtgcgc cacgcgctgc tcgtcgggcc
     3061 gacgagcggc taccgcaccg acaaccgctg cctgctcgac gcgctggaga cgcacaaagc
     3121 ccgctttcgt ggcattgccg tagtcgataa cgacatcggc cgcgacgaac tggccgcgct
     3181 gcgtcaggcg ggtgtcgtcg gcgtggcgtt caacccggcg atggaaggta tcgaactggt
     3241 gttgagcgcc ggtgcattgt tcgccgcgct cgcggatctg gacatggtcg cgcagttgca
     3301 ggttgcgggc gatcagatgg tcagtctcgg gccgtggctc gcgcggcagc cggcaacgct
     3361 tctcgtggat cactgcggcc ggccggacat cgccgcgggt gttgggcagg ccggcttcca
     3421 ggcgctgctg cgcctcgccg ataccggcag gaccaccgtg aagttgtccg gctggcaaaa
     3481 gtattcgcgc gtaccgcatc cttacgagga cgcatggcct tatgcgcatg cgttgttgcg
     3541 cacgttcggt ccgcagcact gcgtgtgggg gtccgactgg ccattcctgc gcgcgcccga
     3601 acgtctcgac tacggcccgc tgctgacgtt gttcggacag atggtgcctg acgtcgcagt
     3661 gcgtcggcgg attcaatggg acacaccgcg acggctcttc ggtttcgatg cgtttccaga
     3721 cacggcagac accacgcata gcgaatccgt tgcttcataa accattttct tgatcgtaaa
     3781 accatggaca aaatgaaacc tttgcgtttt ggcgtactcg gcgccgcgaa gatcgcgcgc
     3841 tcgtttatcg cgggcgtcgg cccttcggag attgtcgata tcgttgctgt tgcgagccgc
     3901 gatctggcga agggcaaaca gtttgcgaac gaactgggcg taccgcacgc gcatggctca
     3961 tatgaagcgc tgatcgacga tcccaatgtc gacgccatct atgtgccgct gccgaacacc
     4021 ttgcacgccg aatgggtcat caaggcgctg aacgcgggca aacacgttct atgcgaaaag
     4081 ccgatcgcga tttcgcagac cgacacgctc gcgatgttcg aagccgcccg tcgcaatcgc
     4141 cgattcctgg cggaagcgta tccgtatcgc gcgcaggagc agacacttgc gctgcgccgc
     4201 ttgctggacg aaggcgcgat cggtcgcgtg cagacggtgt tcgcgtgctt tggcgtcaag
     4261 ttcgccgacc cgtcgaatat ccggctcgat cccgagcgcg gcggcggtgc gctgctcgat
     4321 gcaggcagct acgcgacgaa catggttcgc atggtcgcgg gcgagaagcc gtcgcgcgtg
     4381 aatgcgatcg cgcgctgggc gcaaacgcag gtcgatctca cggtgatcgc caatctcgag
     4441 tttccgagcg gtctgctggg ccagatatct tgcagcttca acacggctta ccatcggcat
     4501 gcgctgatcg cgggcacaga cggcattctc gaaacgacct tcctgaatag cccgcctgat
     4561 gccgggccac ctgaaattca tgtgcgtcgc ggcataccgg cgacgacggt gcgcgaaacg
     4621 atcacgctcg ctggcggcag cggctttcta gccgaagcag agagctttgc gcgtgccgtt
     4681 gcgctggggt ccgagcactg gacgggcagc acggaacagg agtcgatcga aacgattgcg
     4741 acgctcgaag ctattgcgcg cagcatccgt tcgggcaact gggaaaacgt gtagtcaatc
     4801 cgccagatcc ggccgatctg atgatcgagg ccgcagccac aacagacgca tcgaagacgt
     4861 ctgcccgttc tatatcggag acaagcatga aaactgacgc aacattagcg actgccggag
     4921 acctgtctct cgcgtccacg cgccgctcac ggacgcgctt caccattctc gcgctgctcg
     4981 cgatcggcac cgtcatcaac tatctggacc gcacggtgct cggcatcgcg gcgccaagtc
     5041 tgacgaagga tctgggtttg tcggcgggcg tgatgggcat cgtgttttct gcgttttcgt
     5101 ggacgtatac gctctcgcag atacctgccg ggttgctgct cgatcgcctc ggcgcgcgca
     5161 agacttattt tcttgccgtg gcgagctggt cgcttttcat catgctcaac gggctgtcgg
     5221 gcggactggt gtcgctgctg ttttgtcggc ttggactagg cgttgccgag tcgccatgtt
     5281 tctcgacgaa tagccgcgtc gtcggacact ggtttccgca gcgcgagcgt gcgcgcgcca
     5341 ccagcgtcta tcaggtcggg cagtacatcg gtctcgcggt gttcagtccg ctgctgttct
     5401 ggatcatcgc ggcattcggc tggcgtgcga tgttcgtgat cgttggcgcg gtagggctcg
     5461 cgtttgccct cgtctggtgg ctgcgttatc acgagccgca tgagtcacgc aggatgaacg
     5521 acgccgagcg cgaccatatc ggcgcgggcg gaggcctcgc caccgccgcc gacgcgcagc
     5581 ggccgttctc gtggcaagac gtgcgcagcc tgctgggacg ccgcgaaatc tggggcgcag
     5641 cgatcggtca gttcgcgggc aactcgaccg tggtgttctt tctcacgtgg tttcccacgt
     5701 acctcgtgac cgagcgtcat atggcgtggt tgaagatcgg cttctttgcg atcctgccgt
     5761 ttctcggcgc gtcggttggc gtgctgttcg gcggatggct gtcagatttt ctgctgcggc
     5821 gcacggggcg catcaacctg gcgcgcaagc tgcccatcat caccggcctg ctgctttcgt
     5881 cgagtatcgt cgcggcgatc tatgtgaaga gcgatgctgc cgtgattgcc gtactgtcgt
     5941 tggcgttctt tgggcagggc atgggcggcc tgggctggtc gatcattccc gacattgcac
     6001 ccaagcggct gatgggcctg actggcggca tcttcaactt cacggccaac atagccggga
     6061 tcgtcacgcc gatcgtaatc ggcacaatcg tcgcgctcac ggggtcgttc tattgggggc
     6121 tcatgttcat cggagcggtg gccgtgctgg gcgcggcgtc gtacatcttc attctcggcg
     6181 atatcaaacg catcgaattg ccggaggcgc gcgtcgcggg aaggatgtcg tgagggccgc
     6241 tcgagtggtg tatgtttgat ctaccgcgtc aatccttcga gggcagacag atgaggcatt
     6301 ccaactacgc atgtgaagac atcggcaatc tcgtgctgct cgaacacgtc aatctcacga
     6361 tcccggacca gcgtctcgcg accgcgttct atgtgagtgc cctcggtctg acgcgcgatc
     6421 cctttctgat gacgggcgtg accaacatgt ggatcaacgc cggccgttcg caaattcact
     6481 tgccgcacgg cgacgcgcag tgccttcgcg ggcatgtcgg gttgatcgtg ggcaggcgcg
     6541 cgccgctgat cgagcggttg cgcgccgtcg ggcctttgct ggagcagacc cgattcggat
     6601 ggactgagcg ggacgaccgc gtggaagtga tctgtccatg ggggaatcgt tacgactgcc
     6661 tcgatcccga tgcgtgcgcg gcgagcgacc cgtgggcggg catcgatctt ggtatcgtgt
     6721 atgtgcaact cgatgtgccc gtccactgcg cgaggtcgat tgccgcgttc tatcgcgaga
     6781 ttttcgatgc gtctgtcgac ctacgtgagc gcaacgggtc gcggcaagcc gtggtctctg
     6841 tcggtaggca tcaacaactc gtttacgcgg aaacgccctc ggcaagccag gaatacgatg
     6901 ggcaccacat tcagatctat gtggccaatt tctccggacc ttatgaacgg ctcgccgatc
     6961 gggggttgaa ttatggcgag gagcctcacc aatatcgctt cgccgagctc gtcgagcctg
     7021 tgagtggaac gcattgcttc acgcttgaac atgaagtacg cagtctgcgt catccgctgt
     7081 acgcgaggcc gttggtgaac cggaatccgg atgtgaccaa ccgcaattac agaatgggcg
     7141 gcgaggggtt tggcggggtg ttctgatggg tggtcgattc aaatgtcgtg acgcggcgct
     7201 tcactttgcg cctggacaat ctggatgaaa cgctgcgtcg ctggtgacaa atacggatcg
     7261 gtccgatgta ccacgccaaa gttgcgtgtc attgtcgtgt ccggattgaa gacttcgaca
     7321 agcctcggcg tgaagcgggc ttcgtttagg ttgcgtcgcg agatgaaact gagcaggccg
     7381 gtttctgcaa tgagacgcgg caccgcggcg atcgaacttg tcataacggc ggcgcgcggc
     7441 agtggcaagc cacgcgaggc gaacacccgc tcgagccatt gccgcgtcgc gaccgattgt
     7501 gccggcaaca cccagccata accgcacatg tcttccagcg acgcatgctt gcccgcaagg
     7561 ggatgtgtcg cgctcacggc gaccacgacc tgatccgtgc tgatcgggat cgcaacgagt
     7621 tcctcgtcgt gcgtgctgag cgggccaacc acgacatcga gcagacgctt gcgcaatgac
     7681 tcgtgcagca catcgttcat gccgatctgc aattcgatca cgaccgccgg tgcttcctgc
     7741 tgcaacaggc gacagaccgt cggcatgacg aattcggcga ccgtggccgc cgcgcccacc
     7801 cggacgagtc ccgctagccc cttgccgagc gacgagatct gccggtgcgt ctcatcgagc
     7861 gtgcgctcta tcaagagcga gcgctcgtac agcaactgcc ccgcatccgt tagccggata
     7921 ccacggccgc ggcgctcgaa cagatcggca ccgtaggttt cttccagacg gtcgatgcac
     7981 tttgacaatg ccggctgcgt gatatgcaac gcctgcgaag cgcgtccgag atgtcccaat
     8041 tcggcgacct tggcgaagta cagaaggtcg cgcatctgca ggttcatcac cattgcttga
     8101 tttccagagc cagaaagcat tctacgcaaa ccaataactc gcaggaatga aaagatgaaa
     8161 atttgcaaat agacattatg agtctgggtc ttaacaatgc gtgaaatcat tcaaggagac
     8221 gtcgatgtct taccggattc tcgtcacggc accgcggctc gcggctgccg gccaacgggc
     8281 actgtccgat gcgcaatgcg aggttaccta tatcggcaat gcgcaggatg cagccgaggt
     8341 cgagcgcgtc ttgaccacgc agccgatcga cgccgtgatc tcccgcacag tcgatctttc
     8401 cgcctcggcc atcgagggtt gtccgactct caaggtgatt tgcaagcatg gcgtaggcgt
     8461 gaccaatatc gatgtcgacg ctgcgacgcg tgccggcatt cccgtcttca cgacgccagg
     8521 cacgaacacg ttgtcggtcg ccgagctgac cattgcgctg atgctcagcg cggctcgcaa
     8581 agtcaacttt tacgacagtg agttgcgcgc gggccgctgg acgcgcacgg gtgacggcat
     8641 tcaattgcac gaccgcgtat tgggcctcgt cggttacggc cagatcggcc ggaacgtcgc
     8701 gaagattgcc catgcgctcg gtatgcgcgt gcatgtgttc gatcccgcat tgcgcaccgg
     8761 tggcgtcgaa tcgtacgtga cggtcgagcg ctcgccgcag gaactgtacg cccgcgctga
     8821 tgtgttgagc ctccactgcc cggtgaatac gcataagcgc gggatgatcg atgcgcacgc
     8881 catcgccgcg ctgccggatc gcgccatcgt gatcaacact gcacgtggcg aactgatcga
     8941 cgaggctgcg ctggtggccg cgttgcaaag cggcaagctg gcggcggccg gactcgacac
     9001 cttcgcgcat gaaccgctcg gaggggaaga tccgctcctc gcgcttccca atgtcgtgct
     9061 gacgccgcat gtcggcggct cgacacccga tgcgctcgac gccgtagcga cctctgctgt
     9121 cgcaacctgc cttcgttatc tgcgtggcga cgcggtcgat tctcagcgct gcgtcaatcc
     9181 ccacgttctt ctctctgaca aaaaggtgat cgcatgagcg atattcagga tcgggccggc
     9241 tggcccgcag gatttttcgt cggtcgtcgt acggccgtgc cgagcgcggc gcaggtcgac
     9301 gcttttcgaa aggtgccggt cgctcacgcg ggtgattgca tgggccgcag catcggcgca
     9361 accgggctgc ttgcgtacca taactcgctg tcgctcacga tgtgcgggcc ggcgatcacg
     9421 gtgcgcgtgc gtcccggtga caacatgatg attcataagg cgatcgaact ggcgcaaccg
     9481 ggcgatgtca tcgtcgtcga cggcggtggc gatctgaccc aggcgctgat tggcggactg
     9541 atgcgcacct cagcgctggc gaagaagatc ggcggtttcg tcatcgacgg cgccgtccgc
     9601 gatctggccg aatgggccga aggcgtgatt cctgtgtacg ctcgcggcca tacgtttcgc
     9661 gggccgagca aggaggggcc gggtgaagtg aacgtgccga ttgcgtgtgc aggcatggcc
     9721 gtgaacgcgg gtgatctgat gctgggcgac gctgacggtg tgattgcagt tcccgttgat
     9781 caactcgacg cactgctgcc gcttgtgctt gctcatgcca ccaaagaaga gggcattcgc
     9841 gcggcgaacc gcgcgggcag cgccgatccg gagcgcttca acgccctgct gcgtaagaaa
     9901 ggctgcccgg tttaagcgtc gccgccgagc gtgtgcagtc agtgttgagc ccgccgcctc
     9961 ggcccaagcg gactggagaa ggacaaaaaa acaggagaca ggccatgcac gactattcga
    10021 atgtcgccgc gccgggtggc aacggcggca caccgtccac gctgcatatt tcgtcggcgc
    10081 cgcgcgtgac gtttacaccg gaggagctcg tgcggcatat gtcagcgcgg ctcgaccggc
    10141 tgcccgcaag ccgtcccgtc tggctactga tcggtgcgct tgcgctcggc ggctggtttg
    10201 aaatctacga catgtttctc acggcctaca ttggccctgg tcttgtaaag gcaggcatct
    10261 tcaccaaaac cacggcatcg ctattcgatc tgggcgggct cggtgcgttt gtcgcggcac
    10321 tgtttctcgg gcttttcatc ggcacgatcg gcgtagcgtt catcgctgac cggttcggcc
    10381 gccgcaaggt gttcgtcggc gcgctgctgt gctacacgtt cgccgcggtg atgatgtcag
    10441 tgcaacacga tccagtctgg atcgtgttct ggcgcatgat ggcggggata ggcgtgggcg
    10501 cggagctggt caccatcgac gcctacgtgt ccgaattcgt gcccgccagt attcgtggcc
    10561 gcgccttcgc tttcgtacag gcgattcaat acacctccat tccgacgatt gcgtttctgt
    10621 catggcagtt ggtgccgatt gcgccatttg gtcacgacgg ctggcgctgg gtggtgtgga
    10681 tcggctgctc gggcgcggta ttcgtgtggc tgatccggct cggcttgcca gaaagtccac
    10741 gctggctgat acagcaaggt cgcgcagcgg aagccgagac gatcatcgcg cggctcgaac
    10801 gccggatcga acaggcgacg ggcagggcgt tgccgccgcc atcatcctta tccgatacag
    10861 cgaacgtggc gcctgcacgc gttgcgacgc cgagcgccag tgaagacggc ccatggtcgc
    10921 aacgctatcg caatcgcact gtaatgctac tggtgttcaa tttcttccag tcgatcggct
    10981 attacggttt tgcgagctgg gtgccgacgc tgctcgccga gaaaggcgtc gcatttacac
    11041 atagcctgct gtattcgttc atcatcgcca ttgcaagtcc gctcggaccg ctggtggcga
    11101 tgttgttcgc cgacaagctc gaacgcaagt ggctgattgc aggctcggcc gcaaccctcg
    11161 cggtacttgg cgtgatcttc gcgcaacagc gtgatcctgc gatcctcgtc gcgatggggc
    11221 tgttgatgac gctggcgggc aattgcatga cgttctcgta ccgcacgtat caggccgagt
    11281 tgttcccgac gcggatgcgg gcgcgtgcga tcggactcgt gtattcggcg agccgcgtga
    11341 gcgccatgct gtcgggtttc ctgattggct tttcgttgcg tcatacgggg gtggcggggg
    11401 tatttgcgtt gatcgccggc tcgatgttcg tcgtggtatt gacaatcggc ttcttcggtc
    11461 cgcgctcgcg aggcttgagt ctcgaacagg tttcagctta ggagcgcaga tgttctatct
    11521 gaacgagccg ccggcgttga tcgaaacgcg tgtgttttcg agcatgccgg cggcgtttcg
    11581 caacaccggc gaggtcccgg catgggccgt cgcgaacaaa gccggccagc cggtgaacag
    11641 ctttatcgag ggaccggctt tcgatgcaga gggacgcctg tatatcgtgg acattccgta
    11701 cggccgcatt ttccggatcg gcgccgatgg cgaatggacg ctgatcgcgc agtacgacgg
    11761 cgagcccaac gggctgaagt ttcatccgga tgggcgcatc ctcgttgccg actaccgcaa
    11821 cggcctgatg cagctcgatc cgcacaacgg tcgcgtgtcc gcgttcctga caagccgcaa
    11881 cggcgaatcg ttcaagggcg tcaacgatct ggcgattgcg tcgaacggcg acgtctactt
    11941 caccgaccag ggacagacgg gcttgcatga cccgcgcggc cgggtgttcc gtctttctgc
    12001 tacgggcagg ctcgactgcc tgctggataa cggcgtcagc ccgaacggcc tggtgctcga
    12061 tgcggccgaa cgcgtgctgt tcgtcgcgat gactcgcgac aacgctatct ggcgcgttcc
    12121 attgctgccc gatggcggca ccgcaaaagt ggggcgcttc tgcacgatgt tcggcaccag
    12181 tggtcccgat ggtcttgcga tggacgaagc gggcaacctg tatgtcgcgc atgcatcgtt
    12241 ggctcatgtg ctggtgtttg cgccaaacgg cgagtggatt gcgcgcatca aatcctgtac
    12301 ggggacgacg gtcacgaacg tcgcgttcgg tggtccgggg cgccgccagt tgttcatcac
    12361 agaatcgctg acgggaacgg tgctggtcgc cgatgtgccg caagcgggca ttgcattgcc
    12421 tcgcaaagcg gcctgacgat cacgtcgcgg cgcgacgcat gcggagggaa tgaaggagac
    12481 accgcataaa gagagaagtc gagattgtca cggcagtgcc gggtgcgctg aactcagggc
    12541 gcagccattt attgtgtgga ttgaacccga tctggatctg gtggcggtgg tgcgatggat
    12601 tgcggcagac gctgctaacg actttttagg ccgggtgctg gcagccctcc ggtgatgagg
    12661 gaatcggccg tgggtttcgc ctctggccgg tatctacgcg ttatccccta acgacatggt
    12721 gcgatggtat cgctaccatc gcagctatca agaagctaat ccggctgtac gtcgcgcagc
    12781 acaatggtaa cgctaccatg gagacatctg tgtcatcgaa ccgcaaaaca tctgaagagc
    12841 ttcgcagcta tcgctggtat ggcgtgaacg acctccgctc gttcggtcat cgctcgcgca
    12901 cggcgcaaat gggctatcac gcgtcggact acatgggtaa gccggtgatc gccgtcgtca
    12961 atacgtggag cgagatcaac tcctgccata cgcacttcaa gcagcgcgtg gaggaagtga
    13021 agcggggtgt ctggcaggca ggcggatttc cggtggaaat gccggtgatg acgctcgccg
    13081 aaccgttcca gaagccgaca acgatgctgt accgcaactt cctcgcgatg gaaaccgaag
    13141 agctgctgaa gtcttacccg ttcgacggtt gcgtgctgat gggcgtctgc gacaagacca
    13201 cgcccgggtt gttgatgggc gcgatcagca tgaacctgcc ggccatcttt ttgcctgccg
    13261 gaccgatgct gcgcggcaac tggaatggcc ggacgctcgg cagcggctcg gacacatgga
    13321 agtactgggc cgagttgcgc gccggcaaga tcacggaaga agagtggaag ggtatcgaga
    13381 gcggcatcgc gcgctcgccc ggacactgca tgacgatggg caccgcgtca accatgacga
    13441 gcgccgccga agcgctcggc ttgacgttgc ctgggttttc gtcgataccc gcagtggact
    13501 cgcgtcatgc gcaattcgcc tcgctcacgg gccaacgcat cgtcaagatg gtgtggaccg
    13561 atcagaagcc gtcggacatt ctcacggcga agtctttcga taacgccgtc accaccgtgc
    13621 tggccatgtc cggttccacg aacgccatcg tccacctcgt ggcggtggcg cgccgcgccg
    13681 gcatcgatct gacgaccgca cgcttcgacg agctgtcgcg catcacgccg gtgatcggca
    13741 atctgcgtcc gtcgggccag tatctgatgg aagacttttt ctacgcgggc gggttgcgcg
    13801 cgctgctggt cgaacttggc gaactgatcg acggctcaca gatgaccgtc aatggctcga
    13861 cgctcggcga gaacatcgcc ggtgcggaaa tcttcgacga cgacgtgatc cgcaagcgca
    13921 gcaacccggt cgttgccagc gacggcctcg ctgtgctgac cggaaacctc gcgccggacg
    13981 gcgccgtgat caaacccgcg gccatggaag cgcatttgca gaagcatcgc ggtcccgcgg
    14041 tcgtgttcaa ggactacgcg gacatggctg cgcgcatcga cagtgaggaa ctggacgtca
    14101 ccgccgattc ggtgatcgtt ctgcagcacg ccggtccggt gggcgcgccg ggcatgcctg
    14161 aatggggcca actgccgatt ccgcagaagc tgttgaagca gggcgtgcgc gacatgctgc
    14221 gcatttccga cgcgcgcatg agcggcacga gctacggcgc ttgtgtgctg cacgtggcgc
    14281 ccgaatcgtt cgtcggcggt ccgctcgcgc tggtgaaaga cggcgacatg atcgaactgg
    14341 acgtggccgc tcgccgcctg catcttgaag tgagcgacgc cgaactcgcc gcgcgcaagg
    14401 ccgcatggca gccgcccaag ctgccgttca aacgcggttt tggtgtgatg caccagttgc
    14461 atgtcacgca ggcgaacaag ggctgcgact ttgacttcct cgaagagccg gtgagcgaaa
    14521 ccgccgcgca agctgtgcaa tccgcaccga ctgggcagga cacgcaagac acaccggtcg
    14581 cgaagcgcac cgaacccgaa atccattaac acatccgagc ctttcatgac tgcatccgat
    14641 atcagcatca gcgaagcaac cctcgaacaa ctccgccacg tcagcacggc cacgctgacc
    14701 acacaactct tcaagcgcgg cttgcgcaac gtgtttttgc aaggcgtcgc gccactcgtc
    14761 aagccggcac ccggcgcgcc gaatctcgtc ggcccggcgt tcaccctgcg caacattccg
    14821 gcgcgtgagg atctcgacca cctcggcgtg ttccaggacc cggaccatcc gcagcgcaaa
    14881 gccgttgaaa ctgcgccgcc cgggagcgtg ctggtgcagg actgccgtgg cgagcgctcg
    14941 gtcgcctcgg tgggctcgat cctcgcgttg cgtctggcga agcgtggcgt cgccggcatg
    15001 gtgtcggacg gccccgtgcg cgacagcggc acgatctccg gattgggctt gccgctgtgg
    15061 tgcgcaggcg cgagcgcgcc cctgaatctg gcgaaacacc atgccgtcga tatgaacgtg
    15121 ccgatcgcgt gcggcggcgt accggtgtat ccgggcgata tcgtcgttgc cgatgtggac
    15181 ggcgtggtga tcgtgccgcg cgagatggcg gctgacgtcg cgcgcgatgc taccgagcag
    15241 gaacaactcg aagtgttcat cacgcagcgt atcgacgaag gccgcccgtt gcgaggcacg
    15301 tatccgccga gcgaagaaac catggcggcc tacgcgcaat ggcgcgcgca acaaaaatag
    15361 aacgcgcgga ccgtttcgat ccatcgtaag acacaggctc gcgcgcagtg cgcgagcgca
    15421 ggagacactg tgactatcac cacaccggca atcggcgaat cgcgtttgat caatcgcctt
    15481 gccctccggt tgatgccgtt attgggattg ctctatctgg tcgcctatat cgaccgctcc
    15541 aatatcagtt tcgccaagct gcagatactc ggcagtctcg ggttgtcgga agtggcgtat
    15601 ggcctcggcg cgtcgctgtt ctttatcggc tatctgattt tcgaagtgcc gagcaacgtg
    15661 ctgctgcata aatatggcgc gccgcgctgg atggcgcgaa tcatgttcac gtggggcatc
    15721 gtcacgctgc tgctcgcctt cacgcagaac gccacgatgt tctatatctt gcgcttcctg
    15781 ctcggtgcct cggaagccgg cctgtatccg ggcgtgatct attacctgac gctgtggttt
    15841 ccgtcgcgcc accgcgtgcg catgctgggc tatttcacag tcggcagcag tctgggcaac
    15901 atggtcggcg cgccgatctg cggctggctg ctcgacaagg gcggcatcgc gggtctgcat
    15961 ggctggcagc tggtgttcat cgtcacgggc ttgccgtcca tcctgctgac gttcgtggtg
    16021 ttgtacctgc tgccggcgtc gcccaaacag gccacattcc tgtccgccga cgagaagcag
    16081 tggctcgccg acacgctcga cgccgagcga gcccagatca gatcgggcgc aggcgcgcat
    16141 gcatcgctgc tgagcgtact gacggagccg cgcgtgatcg gcatggcgct ctactacatg
    16201 atgctgtcga tttccgtcta cggcgtgagc tactggttgc cgacgctggt gaagggcttc
    16261 ggcgtgagca atacgaccaa cggtctgctg aacatcattc cctggctgat ggcgacgatc
    16321 gtgctcgcgt ggctgccctc gaaactacgc ggcggcaatc gcgcgctcgt cgccatgctg
    16381 gtcgccgcgc tgctcggtgt ggtgtgcttc ctcagcgcgg tattcctgcc gaccaacacg
    16441 ctgcggttcg ccgcgctctg ctttggcgca ccgtgcatgt atctgctgat tccctgtttc
    16501 tggaccttgc cgccgaagtt cctgttcggc gcgcgtgccg cggcgggtat cgcggcaatc
    16561 aattcgctcg ggaatattgg tggtttcatt gcgcagaatc tggtgccctg ggttcgccag
    16621 accacgggca gcgtgaagac gccgatgctg ataccggcgg cttgtctgct gctgttcgcg
    16681 ggcgtcacct tgctgatcct gcggcgcgac aaaggcgtgc gcgtcgcgca gcgcccggtg
    16741 ccggtaaccg actaacgctt ttgactattt gccgtcgcaa cttgcagcag ttgctgttgc
    16801 cgaagaaact cacttcaccg gtgtacagcg gcttcgggtc ggcgcatacc tatcaggtaa
    16861 tcggtgcaag cagggcttat cgcttcggat cggcgaccat cggcgcgatg tattcgaaca
    16921 tccagttcct gcagctcggc gatacggcca attcaggtgt gaactgcctc ggtggaacgc
    16981 tggaacgccg gtcgtcgaaa gcggctaaag ctcgtcgcgc tctacaatag atgcgaccta
    17041 tttattgcca tcctggaccc atgccgaatc acaagccgaa cgccgccagc cccaacgcgc
    17101 ccaaggcgga tgcgcctgcc aaacgcgtac gcggcgggcc caagggcttg cgtattgccg
    17161 acgttgcagc gcaggcaggc gttgcaccga tgacggtgtc gcgggtcctc aacagtcccg
    17221 agacggtggc gcctgagaca ctcgaacggg taaggcaggt cgtgcagaag ctcggctacg
    17281 tgccgaaccg tctggccggc ggtttgtcgt catcacgttc gcggctgatc gcggctatcg
    17341 tgccgaccat cgctcactcg ctgttttctg aaacgatcca ggtgttcagc gagacgatgt
    17401 cgcgagccgg ttatcaggtg ctgctcgcgc tgagcggcta cagtgattcg agtgaggagg
    17461 cgctgctcga cgcggtgctg agccggcgtc ctgaaggcgt gttgctgacc ggcgtcgcgc
    17521 ataccgaatc cttgcgtgag cgcttgcgca acgtcggcat gccgatcgtc gaaacatggg
    17581 atatgaccgg cacgccgatc gatatgctgg tcggcttttc tcactacgaa attggtgccg
    17641 cggtggccga acacttcctg cgtcgcaagg tgcgggcgcc gggcctcata tccgccaacg
    17701 acgaccgcgc gctcgccagg cgcgacggct ttcgcgagcg cctgacgagc gccggcattc
    17761 acgagatcgc cgaagtgctg gtgtcgccgc cgagttcagt ggcgctcggc aaggctgcat
    17821 tgccgacgct gctcgaacag gcacctgagt tggacgccgt gttctgtggc tcggatctgc
    17881 tggcgatcgg cgtgctcggc gcggcgcgac ggctcgggct ggacgtgccg tcgcaattgt
    17941 cgatttgcgg cttcggcgac ctcgaattcg ccacggaaac gacaccgcaa ttgaccacgg
    18001 ttctggttga cggcacgcag atcggactga ctgccgcgcg ttgcctgctc gaccggctgg
    18061 cgggcgacgc caaaaccaaa gtgaccgatg tgggttttca tatcgtcgag cgcgagacga
    18121 cctgatcggc acgccgcgct cgctggtgaa ccgcgccgaa accgctaatc ctcttcgctc
    18181 ttgccctcgc cagtgatcgg cggcccgata tagttcgccc gaggccgcag cagaccgcgg
    18241 ctggccgatt gctcgatgga atgcgccatc cacccaacac ttcgcgcgat cgcaaaaatc
    18301 gagaacactg ctccctcggg taagtggcag tgttcggcaa gcgcggtgag ggcaatgtcg
    18361 atggatggct tcaggcccgt ctcccggccg atatgatcta tcagcttgcg cgtatcttcg
    18421 gaaggctcga atgcgccgag caggttcacc gcgcgcgggt cgccctgcgg gtagagctcg
    18481 tgtccgaagc cgggcaacgc cttgccggcc gacctccagc gcttgacggc tgcctcgacg
    18541 ccgaactgcc gcgcatcgtc cgtcagcgcc tggacacgca cggtcgcatc gccgtgcagg
    18601 gggcccgaca aggttgccag acccgccaac gcgcatgctc cgagcgatgc accggtggat
    18661 gcggcgacgc gcgctgcaaa ggtcgaactc gtcagttcct gatccgcgag caataccaat
    18721 gcgcgacgaa gcaggtcggc ataccgctcg caacgccatg cgcgtgcgat acgcagatgc
    18781 agcggctcct ctgtactggc gagcgtgaca aagctgctgg cgagccttcc gaccagcttc
    18841 tgcgcctcct ggcgcatgct gtcgacatcc agcgtgcgag ctgaatctcc ttcggctgcg
    18901 gcgagcccca ttgcggcgta cgcacgggcc cgcagcggac cggcgttcag caccggttcg
    18961 gaaaccggga agcgcagcgg ctccgccgaa tcccacagga gttgcgcgac ctcctccaac
    19021 gtcagacagg aggcaagctc catcgcattc ttgccccggt agtaaagccg tcctcgaaca
    19081 atggtcgaaa tgcgcgtgtt gatgatcggc tcgccccatg ccatcgtgct ggcggcaatg
    19141 ttcttgcggg cgcgtcccaa agagcgcttc ttgaggagcg cggcaacgtc agattcgcgg
    19201 tagagcttgc ggcttgcatg gtcgggatcc cggcgcacct cgatctgtcc gcggctgacg
    19261 taggcataaa gcgtttgctt gcgcacgccc aggacttcgc atgccgcttc ttgggtaagc
    19321 cagctattca cgttgattat ctcaatcaag gttgacgctc gctgattgcg tgcgtctaat
    19381 gcaaaacata caaccactct aaagcagatc acgcgcgcgc aaccctacgc gggcgtgtcg
    19441 atgcaggcaa tcatagcgtg tgcgtttcgt atgcaccatg cagccggcac gtgagacaaa
    19501 aaaaggagga gatctcgatg ttctcagaca ggacagctca gtacgcggcg acggattccg
    19561 ttcgcgtgac ccatacacgc ttcgcgcttc tcgcgacgat attgctggtg gcgaccgtgg
    19621 cctatgccga ccgcgcaatc ctgtcgatcg ccgggccagg catcgcgaag gaattcggcc
    19681 tgaatcacat tcagctgggc tatatcctgt cggccttcag ttgggcgtac gtgatcggac
    19741 agattcccgg tggcctgctg ctggaccggc tcggtaccaa agccgtgtat ggcgcaacgc
    19801 ttgttttgtg gtccatcgcg acaattctgg ttggcttcat cggcaagatc acgtccgacc
    19861 tttcagttgc gctcggtctg ctgtttgcgc tgcggttcgc gctcggcttt atcgaagcgc
    19921 ctagcttccc ggccaacagc cgcgtgaccg tcatgtggtt tccgaaagac gagcgcgggc
    19981 tcgcgacctc gttgttcgcc tcggcctcgt acttcgcggt ggcgatcttc tcgccgctcg
    20041 cggggtggtt ggtggcgaag ttcggctggc ccgcgccgtt ctttgccctc ggcctgatcg
    20101 gcattgcctc ggccgggctc tgggcggtgg tcatgcacga gccgcgcaaa catccccgcg
    20161 cgtcgcaagc cgaactggac cacatcgtgg cgggcggcgc gatggtcgat atcgattcgt
    20221 cgcaggagcg gctgtcccgt cccgtggtgt cgtcaaaagt gttgcgcgtg ctgctgggta
    20281 accggatgct gtggtgctcc tacatcggcc agtattgcgc cattgcgctg agctactttt
    20341 ttatcacatg gttcccgatc tatctggttc aggcgcgcgg catgaacatc atgcaggccg
    20401 gactggccac catgctgccg gccatctcgg gatttctggg cggcattgcg ggcggcgtga
    20461 tttcggacct gctgattcgt cgcggctgga gtgtgtcgtg ggctcgcaag acgccgtata
    20521 tcgtcggcat ggcggtgggc tgctgcctgg ttttctccgc cttcactgaa agcaacaccg
    20581 tgatcgtcct gctgatgacg atcgcgttct ttggcaaagg caccgccgct ggcgcgggca
    20641 cgtgggccat cgtcagcgat accgcgcctc gggaagccgt gggccttgcc ggtgcgatat
    20701 ttaattgcgt gggcaatatc ggcggtatcg ttacaccgat cgtcttcggc tacatcgttg
    20761 aggcaacggg cgactacacg gccggccttt attttgtagc tgcgcattgc ctcgtggccg
    20821 cggtcgtcta tctgttcttc atgggcaaaa ttgagcgcgt gaagatgagc tgaatggaat
    20881 gcatcgacgc ggcggcgtgt tagcgtgctg ccgcggatga atcgttaaac tggatgaaca
    20941 actaaacgca ctatggaaaa gccgatcatt ctgaaagcgg ccgagttgcc ggattggacc
    21001 acggccgagc tcaacgcact cttcaccgta ctcgaactgc ctgagggacc cgcagcagct
    21061 tcggcgttcc tgaaggagcg cggtcctgct attcggggaa tagcgttgcg caaaaccaag
    21121 gtcgacgctg cgtttctcga cgctgttccc gcgctggaaa ttatttcgag ttacagcgca
    21181 ggcctcgaca acgtcgatgt gcaagccgtg aaggcgcgag gtatcgttat cgaaaactcg
    21241 tcccatgtgc tcgcggagga cgtggcgaac accgcgctcg gactggcgct tgcagtcacg
    21301 cgcgatttcg tgaaagcgga tgcgtatgtt cgggcaggga aatggccgga gcacgggcaa
    21361 tttccgctgg gccggtcgat ctcgcgcatg aacgtcggaa tcgtcggcct tggcatgatc
    21421 ggctcggcca tcgcacggcg cctgcaggcg ttcggctcgc gcgtggcgta cttcgggccg
    21481 acccgcaagc ccgtggatat cccgtactac gatagcgtgg tcggcctcgc ggccgcttgc
    21541 gatatgttga ttctggcctg ccccttgtcc cgggctaccc atcatctggt ggacgcaacg
    21601 gtgatcgatg cgctcggtcc cggcggcttt ctggtgaaca tctcacgtgg ccccgtggtc
    21661 gacgaagcgg agctcatcgc cgcgctgggc gccgatcgga tcgccggtgc ggcgcttgat
    21721 gttttcgagc gagaaccc
//
LOCUS       DATASH010000129         2336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1007270,
            whole genome shotgun sequence.
ACCESSION   DATASH010000129 DATASH010000000
VERSION     DATASH010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007270"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VHV99_03835"
     CDS             complement(<1..345)
                     /locus_tag="VHV99_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX3634611.1"
                     /translation="MKTLEYVPVPAFEDNYIWLVSDGHNAVVVDPGDAAPVEAYLTKR
                     GWRLTAILLTHHHHDHVGGVAKLLVSQSAGNSANAEIPVIGPAGEAIEHLTRRVKQGD
                     HVSIASPALECSV"
     gene            364..1209
                     /locus_tag="VHV99_03840"
     CDS             364..1209
                     /locus_tag="VHV99_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3634612.1"
                     /translation="MSERTIIDWPAWTLSAPGRYVLEWEQAQLDRVVSDIFGYHALQL
                     GLPGLDALRENRMPYRGLVLDTASAASAPFTLPPPVSADARPGVTSLPSASRGLHAPE
                     GRSAVWCDLLDLPFEAQSVDLLVMPHTLEFTSDPHRLLREAERVLMPEGQLIILGFNS
                     LSLWGARQSLGKVAGKPFVPSAHEMIAFTRIKDWIKLLGFDLERGRFGCYRPPLLTEQ
                     WLARYQFMEAAGDRWWPIFGAAYMITAIKRVRGMRLIGPRKLKKPALAPGLTPVAAPH
                     TRNEH"
     gene            1209..1667
                     /gene="rnhA"
                     /locus_tag="VHV99_03845"
     CDS             1209..1667
                     /gene="rnhA"
                     /locus_tag="VHV99_03845"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEX3634613.1"
                     /translation="MTLDTTDKVIDIYTDGACKGNPGPGGWGALLRFGDQEKELFGGE
                     TPTTNNRMELMGVISALEALKRPCRVVIHTDSQYVQKGISEWIHGWKKKNWMTAAKTP
                     VKNADLWKRLDALVVQHQIEWRWVKGHSGHPENERADALANRGVTSLAEG"
     gene            1679..>2336
                     /gene="dnaQ"
                     /locus_tag="VHV99_03850"
     CDS             1679..>2336
                     /gene="dnaQ"
                     /locus_tag="VHV99_03850"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEX3634614.1"
                     /translation="MRQLVLDTETTGLNARTGDRIIEIGCVELVNRRLTGNNLHFYVN
                     PGRDSDPGALAVHGLTTEFLSDKPKFADIAAELRDFVAGAELIIHNAPFDVGFMDVEF
                     GLIGMPGVKEVCGGVIDTLVQAKQMFPGKRNSLDALCDRFGISNAHRTLHGALLDSEL
                     LAEVYLAMTRGQESLVIDMLGEMPAMGEGHAPRIALDDLQLPVLVASEEELSVHLAVL
                     D"
BASE COUNT          460 a          698 c          725 g          453 t
ORIGIN      
        1 aaccgagcac tccagcgcag gactcgcgat actgacgtgg tcgccctgtt tcacgcgtcg
       61 cgtcagatgc tcgatagcct cgccagccgg gccgatcacc gggatttccg cattggctga
      121 attcccggcc gattggctaa cgagcagttt tgcgacgccg ccgacgtggt cgtgatggtg
      181 gtgcgtgagt aaaatagcgg ttagccgcca accgcgtttc gtcaaatagg cttcgacggg
      241 cgcggcgtcg ccaggatcga ccacgacggc attgtgaccg tccgacacca gccagatgta
      301 gttgtcttcg aacgccggaa ccggcacgta ctcaagcgtt ttcataaggc gactcacccg
      361 aacatgtcag agcgaacaat tatagactgg cccgcctgga cactttccgc gcccggccgc
      421 tatgtgctcg aatgggagca ggcacagctc gaccgcgtgg tgtcggatat tttcggctat
      481 cacgcgctgc aactcggcct gcccgggctt gacgccttgc gcgagaatcg catgccgtat
      541 cgaggcctcg tgcttgatac cgccagcgcc gccagcgcgc ctttcacgtt gccgccgccg
      601 gtttcggccg acgcgcggcc gggcgtgacg tcgctcccgt cggcaagccg cggcctgcac
      661 gcgccggaag gacgcagcgc cgtgtggtgc gacctgctcg atttaccgtt cgaggcgcag
      721 agcgttgact tgctggtcat gccgcatacg ctggaattta cgagcgatcc gcaccgttta
      781 ttgagagaag ccgaacgcgt gctgatgccc gaaggacaat tgatcatcct gggctttaat
      841 tccctgagcc tgtggggtgc gcggcaatcg ctcggcaagg ttgccggcaa gccgtttgtc
      901 ccgtccgcac acgaaatgat cgcgttcacg cggatcaagg actggatcaa gctgctcgga
      961 ttcgaccttg aacgcgggcg cttcggctgc tatcgtccgc ctctcctgac cgaacagtgg
     1021 ctggcgcgct atcagttcat ggaagctgcc ggcgaccgct ggtggccgat cttcggcgcc
     1081 gcctacatga tcacggccat caagcgcgtg cggggcatgc gcctgatcgg gccgcgcaag
     1141 ctgaaaaaac ctgccctcgc gccggggctc accccggtcg ccgcaccgca tacacgcaac
     1201 gagcattgat gaccttagac accacagaca aagtaatcga catttatacc gacggcgcct
     1261 gcaaaggcaa tcccggcccc ggcgggtggg gcgcactgct gcgtttcggc gatcaggaaa
     1321 aggaattgtt cggcggcgaa acgccgacga ccaacaaccg catggaactg atgggtgtga
     1381 tctccgcgct cgaagcgctc aagcggccgt gtcgcgtggt cattcacacc gactcgcaat
     1441 atgtgcagaa gggcatcagc gagtggattc acggctggaa aaagaaaaac tggatgacag
     1501 cggcgaaaac gccggtcaag aacgcggatc tctggaagcg gcttgatgcg ctggtggtcc
     1561 agcatcagat tgaatggcgc tgggtcaaag gccattcggg ccatccggaa aacgaacgcg
     1621 ccgacgcgct tgcgaatcgc ggcgtcactt cactcgccga aggttaattt ttatttctat
     1681 gcgccaactc gtactcgata cagaaaccac cggcctcaac gcccgcacgg gcgaccgcat
     1741 tattgaaatt ggctgcgtcg agcttgttaa tcgacggctc actggcaata acctgcactt
     1801 ctacgtgaat ccgggacgcg acagcgatcc gggcgcgctc gcggtgcacg gcctgaccac
     1861 cgaatttctc agcgacaaac ccaagttcgc cgatatcgcc gccgagttgc gcgacttcgt
     1921 ggcgggcgcg gagttgatca tccacaacgc gccgttcgac gtcggtttta tggacgtgga
     1981 gtttggtctg atcggcatgc ctggcgtgaa ggaagtatgc ggcggcgtga tcgacacgct
     2041 cgtgcaagcg aagcagatgt tccccggcaa gcgcaattca ctcgatgcgt tgtgcgaccg
     2101 gtttggcatc agcaacgcgc ataggacgtt gcatggcgcc ctgctcgact cggaattgct
     2161 ggcagaagtt tatctggcga tgacgcgtgg ccaggaaagc ctggtgatcg atatgcttgg
     2221 cgaaatgcct gcaatgggcg agggtcacgc gccgcgtatt gcgctcgatg atttgcagtt
     2281 gccggtgctg gtggcgtcgg aggaagagtt gagcgtgcat ctcgcggttc tcgacg
//
LOCUS       DATASH010000130        20172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5358783,
            whole genome shotgun sequence.
ACCESSION   DATASH010000130 DATASH010000000
VERSION     DATASH010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 20172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20172
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5358783"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VHV99_03855"
     CDS             <1..286
                     /locus_tag="VHV99_03855"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203522.1"
                     /note="One of the components of the high-affinity
                     ATP-driven potassium transport (or KDP) system, which
                     catalyzes the hydrolysis of ATP coupled with the exchange
                     of hydrogen and potassium ions; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit B"
                     /protein_id="HEX3634615.1"
                     /translation="AIIPAAFATTYPALNALNVMHLATPASAIMSAVIFNALIIVLLI
                     PLALKGVKYRALGAAILLRRNLLVYGLGGIIVPFIGIKLIDMVLAAFGWV"
     gene            317..895
                     /gene="kdpC"
                     /locus_tag="VHV99_03860"
     CDS             317..895
                     /gene="kdpC"
                     /locus_tag="VHV99_03860"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HEX3634616.1"
                     /translation="MKNLFRPLIVIFAVLTALTGLAYPAVMTAVGQAAFSDQANGSLL
                     EQGGKVVGSKLIGQQFDAPQYFWGRLSATSPMPYNATNSGGSNLGPTNPALLDEVKGR
                     IDALKAAGTDMSNPVPVDLVTSSGSGLDPEISPAAAAYQIERVAKARKLTTNDVQALV
                     DRYTSGRQFGILGEPRENVLHLNLALDEMKHG"
     gene            1154..3988
                     /locus_tag="VHV99_03865"
     CDS             1154..3988
                     /locus_tag="VHV99_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HEX3634617.1"
                     /translation="MNRPDPDELLDKLQRDEEKRQRGRLKIFFGASAGVGKTYAMLQA
                     ARRRADEGADVVVGIAETHGRSETAALLEGLDVLPLAHIEYRGRKLGEFDLDAALARK
                     PQLILVDELAHSNVQGARHLKRWQDVYELLDAGIDVYTTVNVQHLESLNDVVGQITGI
                     RVWETVPDRVFDRADEVTLVDLPAEELLDRMRDGKVYLPQQAERAVRNFFRKGNLIAL
                     RELALRRTADRVDAQMREYRADRSIQRIWQARERLLVCVGPGPEAPMLVRAAARLAAS
                     LKADWIAVYVETPALQRLPDARRERTLNALKLAAELGAETATLAATDAVAALIGYAQA
                     RNVSKLVAGASARTGFMRWLRRPLGERIAERAGDLDLTLIRASSERDGGEHRRLLNTT
                     ANAWREVLSTARERRSPPRAYGIALAICAAITLLSSQLIDRIDLANLVMLYLLGVIFT
                     AVKLGRGPGVVLSFMSVAAFDFFFVPPRMSLSVSDTQYLLTFFGMLLTSLVISHLTSS
                     LRREASVAQRREQRTGAMYAMARELAAALTTEQIVGIGSRHVSEVFGARVAILLPDSA
                     DQVKQKIEDPDAAITLEGELLDSDVGQWVYDQQKPAGQGTDTLPAAAARYLPLKAPMR
                     TRGVLAVVMRDERELDVPEQQRMLDAFAAQIALALERVHYVDIARDALVNMESERLRN
                     SLLSAISHDLRTPLTTIVGFSSMLAQSREAHPGAQPGDDLVEAIHEEALRMTGIVTNL
                     LDMARLQAGSLQLNRQWTLLEETVGAALRACKRVLANHPVQVKLPADLPLLHLDAVLM
                     ERLFSNLFENAAKYTAPDTPLTISAQGLDADGKSFVRVAVDDTGPGLPAGMEARVFEK
                     FTRGEKESAKPGIGLGLAICRAIVEAHGGTIGALNRLSADGRVEGARFWFTLPVETPP
                     EAPDALEDEGADALPDLDSTLIQHSKPSHE"
     gene            3981..4679
                     /gene="kdpE"
                     /locus_tag="VHV99_03870"
     CDS             3981..4679
                     /gene="kdpE"
                     /locus_tag="VHV99_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006759840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HEX3634618.1"
                     /translation="MSDPSITVVLIEDEKQIRRFVRTALEGEGIVVHDAETGKQGLVE
                     AATRKPDLIIVDLGLPDTDGLDVIRELRGWSELPVIVLSARTQETEKVAALDAGADDY
                     LTKPFGVSELLARIRAHMRRRNQGGANESPVVRFGAVTVDLALRQVSRDGVAVHLTPI
                     EYRLLATLVRHAGRVLTHRQLLREVWGPSHVESHHYLRIYMAHLRGKLERDPAQPEHI
                     VTETGVGYRLVGAV"
     gene            4903..5223
                     /gene="sugE"
                     /locus_tag="VHV99_03875"
     CDS             4903..5223
                     /gene="sugE"
                     /locus_tag="VHV99_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HEX3634619.1"
                     /translation="MSWFLLLIAGLLEVAWAAGLKTSEGFTRVWPSVFTVVTALGSFV
                     LLAMAMRQLPLGTAYAVWTGIGAVGAFIFGIVMMGEALTPARVASAALIVIGLIGLKL
                     SSGH"
     gene            5587..6198
                     /locus_tag="VHV99_03880"
     CDS             5587..6198
                     /locus_tag="VHV99_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HEX3634620.1"
                     /translation="MLESLSLAAGLSWGSGLRLYLTVLLAGVFARLGLVHLPDTLSAL
                     SSPWVIGVAGVLTITEFLADKIPAFDSLWDAIHTFIRIPAGAMLAAGALGHADPALLT
                     VAALAGGTLAGTAHLAKAGTRALINLSPEPLSNVVTSTAEDGLVFGGMLLALFVPLLF
                     LVLMVGFLMLASWALPRLWRGVQGGFRGMATHMVSRFARSRHD"
     gene            6261..8777
                     /locus_tag="VHV99_03885"
     CDS             6261..8777
                     /locus_tag="VHV99_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX3634621.1"
                     /translation="MDVLRQAIRMTARDWRAGELTMLLLALVLAVAALSSVGFLADRL
                     HQGLERDARRMIAADFIVRADHPVDPQFAQQAKALGLNTASTAIFPSMINSTGAQPVS
                     RLAAIKAVSPGYPLRGALRIAAAPGAADHATRSIPAPGTVWVDQALLDALKLHVGDAV
                     KVGTRSFSIGAIITRELDRGFSFVNFSPRLMLRADDVQSTGLITFGSRVTYRLLVAGT
                     DASVARFAQFAHDRVDGGKMRGVALESLQDGQPQVRQTLDRASHFLTLVSLLTALLAA
                     VAIAMAAHRYMRRHLDGCAAMRCLGVSQRTLRTLFTLEFVALGLIGGVLGVALGYLGH
                     MALLTWLGSLIDVALPYPSVWPALEGIAAGLVLLLGFALPPLLPLTRVPPVRVLRREW
                     GEAGRTAWAAYALGIALFAALLIVAAGELKLGGIVAGGFAGGLLVFACIARAALWGAA
                     RLVRSERVNAGIGWRYALASLERRSGASALQITALGIGLMCLLLIAMTRDDLVAGWRK
                     STPPDAPNEFIIDIQPDQRDAVTQYLNTHGVPGTVLSPMVRGRLIAINGKPVNPDSFK
                     GADARRLVDREFNLSYTTELPEDNRLSAGSWYGDTKTPQISIEQGLAKLINVKPGDTL
                     RFDVTGLQIDAPVTSVRKLDWGSFKVNFFVLMPPAALQDFPATFITSFHLPPEKQSTI
                     DGLIAAYPNLTAIDTGPILAQVQRVLQQVIGAVQFLFAFTLAAGVLVLYAALAGTRDE
                     RMRESTLLRALGASHRQVRAVQVAEFVSVGALAGLMAAVGAQVIGWLLATRVFEFYLT
                     FDPWLLPAGVAAGIACAGVGGWLSLRHVLARPALQSLRDA"
     gene            8807..9220
                     /locus_tag="VHV99_03890"
     CDS             8807..9220
                     /locus_tag="VHV99_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017331548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HEX3634622.1"
                     /translation="MSDLDDEVSAAQPTAFELVGGETRVRELIDRFYDLMDLEPEFAG
                     IRALHPESLDGSRDKFFWFLCGWMGGPDHYISRFGHPRLRARHMPFPIASSERDQWLR
                     CMAWAMEDVGLSEPLRERLLASFFETADWMRNRNG"
     gene            9213..9815
                     /locus_tag="VHV99_03895"
     CDS             9213..9815
                     /locus_tag="VHV99_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HEX3634623.1"
                     /translation="MVDGAAGLEAEARAVLDCWFGAPDSSTFGHARKCWFSRDKAFDA
                     MLLERFGTLIDAAREGSLDGWMATSLGALALVIVLDQFSRNCHRHTPRAFAADHQALR
                     IAQRMIASGADLLLPGAHHRAFAYLPFEHDETLVSQHESLRLFEQLKAEPGGASYYSS
                     ALRHARIIERFGRFPHRNAQLGRQSTVEETAFLKEPRSSF"
     gene            complement(10073..12064)
                     /locus_tag="VHV99_03900"
     CDS             complement(10073..12064)
                     /locus_tag="VHV99_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEX3634624.1"
                     /translation="MHEKSSAPWRTGNVAIATPTPAGPADAAPDTRPAVAHAAESGAA
                     LSAAHAVKAAAPAKPAAPAGPTEPTALAPASRGAQRLRALAAVRPLMWLAALAILSAW
                     LLPGILGHEPWKQDETYTFGIVQHMLETGDLVVPTNAGQPFVEKPPIYDWVAAGLAWM
                     FGRYLPLHDAARLASALFAGLTVYYTARAARRAVTASSWFDIRVIGTLALFAGTLVVV
                     KHVHDMMTDVALMAGAALGFCGLFELVLVHLRDEARLLPVDARRRRQAIFSGAAMFGA
                     GVGVSLLAKGLFVPLVFGVTTVAVLVLYPACRTRSFAGALGIAALVSSPFALIWPICF
                     YLRSEALFKVWLWDNNVGRFFGFSVAELGSENESRLFVLRTVLSVGFPVVPLALAALA
                     GGGWRRWRDPRVALPVIFAGAGFAVLQSSATVRELYILPFIAPLALVAMQGIEKLPRR
                     LHASWDIASRVLFGSAAALAWIIWSIMSDPANTHASLRRLGRWLPLDWLLPVKPGLIA
                     AALLMTLCWLWLLPVFKYTGKWRGALSWCAGAILVWGLVSTLLLPWLDYAKSYRSVFE
                     NLGAQMNIEWNDGDCMASAGLGESEAPMLYYYTGIQHQPTANTATTECTWLIEESRRD
                     TARTPAGDWQLFWSGARPGDTDEVLRVFVRTPAPPARGE"
     gene            complement(12554..13300)
                     /locus_tag="VHV99_03905"
     CDS             complement(12554..13300)
                     /locus_tag="VHV99_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015600657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3634625.1"
                     /translation="MTKTVLITGGSRGIGRATARLLGARGWSVGVNYATNLAAAQETV
                     AEVERVGGHALPIAGDVASEADVIAMFDALQQKFGRLDALVNNAGIVAPSSQLADMDL
                     ARLKRMFDVNVLGAYLCAREAARRMSTTRGGAGGVIVNLSSAASRLGSPNEYVDYAGS
                     KGAVDTMTIGLAKELGPQGVRVNAVRPGLIDTEIHASGGKPERAAQLGATTPLGRPGR
                     ADEVAESIVWLLSDAASYVTGALLDVSGGR"
     gene            complement(13396..13980)
                     /locus_tag="VHV99_03910"
     CDS             complement(13396..13980)
                     /locus_tag="VHV99_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX3634626.1"
                     /translation="MKSVCVYCGSSDGAKPLYAEAARAFGRALVAADLALVYGGGKVG
                     LMGVIADTVMAEGGRAIGVIPELLVNKEVGHNGLTELHVVPDMHQRKKMMADLSDAFV
                     AMPGGVGTLEELFEVYTWAQLGYHQKPVAVLNIDGFYDPLIALLKHTVEEGFMRQTYF
                     DILQMEADPVALIDKLQRYQPPANDKWAIKRDAV"
     gene            complement(13983..14708)
                     /locus_tag="VHV99_03915"
     CDS             complement(13983..14708)
                     /locus_tag="VHV99_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX3634627.1"
                     /translation="MEAKPPRRTRERILELSLKLFNEIGEPNVTTTTIAEEMEISPGN
                     LYYHFRNKDDIINSIFGQFEQEIEKRLRFPDDHRATIDEMWSYLQYMVDFTWRYRFLY
                     RDLNDLLARNRTLETHFKQIISHKVRFASQFCEQLVADGEMVATPEELQVIATNIGVI
                     ATYWLSYQFVMNPRKYNEQETIRAELHQVSVQIVSLMAPYLRGRSRQIFDDLVSGKLP
                     KREFYDYLPPKEGAVPRNEPKDT"
     gene            complement(14865..15542)
                     /locus_tag="VHV99_03920"
     CDS             complement(14865..15542)
                     /locus_tag="VHV99_03920"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEX3634628.1"
                     /translation="MRTRNSTAAHASNGAMRAVDAGTDTAGIEPFDDVREHDPEHDTE
                     RHTGHAAPNHANHANGLRGMNGASLERSDSPDSDLHHEPLSPDDPLAPLPFNPYKGNR
                     GLTRAWHAMKNSLAGFRVAIREESAFRQELTLAAILIPCGVLVPVDPVSRVLLLGSVL
                     LVLIVELLNSSVEAAIDRISLERHELSRRAKDLGSAAVMVALGMCLMTWGLIVGPLVV
                     RWVKAWL"
     gene            complement(15563..16594)
                     /locus_tag="VHV99_03925"
     CDS             complement(15563..16594)
                     /locus_tag="VHV99_03925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX3634629.1"
                     /translation="MKIMIVTDAWEPQVNGVVRTLKNTTRELTALGHRVDLLTPLEFK
                     TIPCPTYPEIRLSLLPRRKLRQRIGEFAPDALHIATEGPLGMAARAYAIQHKLPFTTA
                     YHTRFPEYVQARFGIPLAATYKFLHWFHKASLAVMAPTPVVKTDLEKFGFTNVVLWTR
                     GVDLDIFHQMDSKVLNTARPIFLYVGRVAVEKNVEAFLKLDLPGSKWVAGEGPALAEL
                     KSRYPQANYLGVLTQAELAKVYAAADVFVFPSRTDTFGLVLLEALACGTPVAAYPVTG
                     PIDVLGDGGAGAMHEDLREACLEALKIDRSHARAWAERFSWAAASEQFAAHLKPLPRA
                     SYSEESAAA"
     gene            complement(16625..17566)
                     /locus_tag="VHV99_03930"
     CDS             complement(16625..17566)
                     /locus_tag="VHV99_03930"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEX3634630.1"
                     /translation="MDPKTSATSLFRQTGPSVDPVAFRPEPDPGHGLPLPVPSLPLDA
                     HAGHHDDEHAEPHRYRTIWLSDIHLGSSGCQAPYLLDFLRHNESEYLYLVGDIIDGWQ
                     LKKGWYWPQAHNDVVQKVLRKARKGTQVIYVPGNHDEAARQFCDLAFGDIHVRGEAFH
                     TTLAGKRLWIVHGDLFDGVIQHAKWLAYLGDTLYTMILILNRWFNRIRSRLGFPYWSL
                     SQYLKHQVKNAVNFISSFERVMTDEARRRGCDGVVCGHIHKAEIRDIDGVLYCNDGDW
                     VESLSALVETYEGELKVIYWTVMRAPEVGVQKARATA"
     gene            complement(17851..18381)
                     /locus_tag="VHV99_03935"
     CDS             complement(17851..18381)
                     /locus_tag="VHV99_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX3634631.1"
                     /translation="MSQPLATSTLPAASSGTAPTVRRRLAALLYEAVILFGVVFVAAY
                     LFSTLTQQRNGLTHHNLLAAWIGLVVGLYFVWFWTHSGQTLPMKTWHLRVIAADGAPL
                     STGRAIVRYVLAWLWFLPPLALHPLLGLAVPQTLVIAAIWFVLWAATGRFDSQRQFLH
                     DRLAGTRVVSVVRQAN"
     gene            complement(18393..19145)
                     /locus_tag="VHV99_03940"
     CDS             complement(18393..19145)
                     /locus_tag="VHV99_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HEX3634632.1"
                     /translation="MSYKRGLAVVFGCTIAALVSFAATYPRFYPSPSPVSAPAASGNA
                     TAKAPTLAVELPSLPTSNSPLAWSRLSTAEHVALAPFASQWDSFSEERKRKWLKVASR
                     YAKLSPDAQKRLHDRMTEWTRMTPDQRRVARENYQVSKELPRETRQNAWKAYQQLPEE
                     QKERLAASERKRRPSVVSAPPSGKSEIKGLDRLVNAREHGASGAAAVSAPLAVPLPSP
                     ASTPAIPAAGSFVPATPVPVSPSEAPSIFHGS"
     gene            complement(19162..19590)
                     /locus_tag="VHV99_03945"
     CDS             complement(19162..19590)
                     /locus_tag="VHV99_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3619 family protein"
                     /protein_id="HEX3634633.1"
                     /translation="MSSPETKELEFARQVRRALDETAASIPAATVDRLAAARRAALAR
                     KKPETVSAPVFVPAFAGAGMPAGMPQVDLPQRRRSPLRRFALAWPLVALVVSLVGIAY
                     WEDQQRTAELADIDAAMLSDDLPLNAYLDHGFNAYLSRAH"
     gene            complement(19587..20150)
                     /locus_tag="VHV99_03950"
     CDS             complement(19587..20150)
                     /locus_tag="VHV99_03950"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX3634634.1"
                     /translation="MASDKELADFLAGVERRAFKQTVYAVRDDDASLDIVQDAMIKLA
                     EKYGDRPPAELPLLFQRILQNAMHDYFRRAKVRNTWVSLFSSLGNADDDEFDPLETFE
                     AQQGSAGSESNEQKLEREQVLQLIDDEIQKLPARQREAFLMRYWEDMDVAETAAAMGC
                     SEGSVKTHCSRATHTLAQALKAKGITL"
BASE COUNT         3463 a         6252 c         6788 g         3669 t
ORIGIN      
        1 cgcgattatt ccggctgcgt tcgcgaccac ctatccggcg ctgaacgcgc tgaacgtgat
       61 gcatctggcc acgccggcct cggcgatcat gtcggcggtg attttcaatg cgctgatcat
      121 cgtgctgctg attccgctgg ccctcaaggg cgtgaagtac cgggcgctgg gcgcggcgat
      181 cctgctgcgc cgcaatctgc tggtctacgg tctgggcggg atcatcgtgc cgttcatcgg
      241 catcaagctg atcgatatgg tgctggccgc gttcggctgg gtttgagaag cggctgttta
      301 agaggaatta ttcatcatga aaaatctgtt ccgtccgttg atcgtcattt tcgcggtgct
      361 gaccgctctc accggacttg cttatcccgc cgtgatgact gcggtcggtc aggcggcttt
      421 ctccgatcag gccaacggca gcctgctcga gcagggcggc aaggtggtcg gctcgaagct
      481 gatcggccag cagttcgatg cgccgcagta cttctggggc cgtttgtcgg cgaccagccc
      541 gatgccgtac aacgcgacca actcgggcgg ctcgaacctc ggcccgacca acccggcgct
      601 gctcgatgaa gtcaaaggcc gtatcgatgc gttgaaggct gccggcacgg atatgtcgaa
      661 tccggtgccg gtcgatctgg tgacgtcttc gggcagtggt ctcgatccgg agatcagccc
      721 ggcagccgcc gcttatcaga tcgaacgcgt cgccaaggca cgcaagctca ccacgaacga
      781 cgtgcaggcg ctggtcgatc gttatacgag cggccgccag ttcggtattc tgggtgagcc
      841 gcgcgagaat gtgctgcacc tgaatctggc actggatgag atgaaacacg gttgagggtc
      901 gccgaacgct aaccagcgtc aataagcgat ctcgatggcg gtgacccgag gaggcggcgt
      961 ccatgcggcg ccgccttcta tttgttttcg cgccgctgtg gtggcaggct gcctggcgca
     1021 cgtgtagcgc tacttgatcg gcgggcggca cggctgccat tgccgctgtc atttcctcag
     1081 ccaattgcgg gctggccgcc gccgtgccac catgcacgca ctatcgctcg ccttactatg
     1141 aaaagcattg agcatgaacc gccccgatcc tgatgaactg ctcgacaagc tgcaacgcga
     1201 cgaagagaaa cgtcagcgag gcaggctgaa gattttcttc ggcgcgtcgg cgggtgtcgg
     1261 caagacctat gcgatgttgc aggccgcgcg gcgccgtgcc gatgaaggcg cggacgtggt
     1321 ggtcggcatc gccgaaaccc atggccgcag cgaaaccgct gcgctgctcg aaggcctcga
     1381 cgtgctgccg ctcgcgcata tcgagtatcg cggccgcaag ctcggcgagt tcgatctcga
     1441 cgccgcgctc gcgcgcaaac cgcaactgat tctggtcgac gaactcgccc attcgaacgt
     1501 gcagggcgca cggcatctga agcgctggca agacgtctac gaactgctcg acgcgggcat
     1561 cgacgtctac accacggtca acgtccagca cctcgaaagc ctgaacgacg tggtcggcca
     1621 gatcaccggc atccgcgtct gggagacggt gcccgaccgc gtgttcgatc gcgccgacga
     1681 agtcacgctg gtcgacctgc ccgccgagga actgctcgac cggatgcgcg acggcaaggt
     1741 gtatttgccg cagcaggccg agcgggcggt gcgcaacttc tttcgcaagg gcaacctgat
     1801 cgcgctgcgc gagctggcct tgcggcgcac agcggaccgt gtcgatgcgc agatgcggga
     1861 atatcgcgcc gatcgttcga tccagcggat ctggcaggcg cgtgagcgct tgctggtgtg
     1921 cgtgggcccg ggtccggaag cgccgatgct ggtgcgggcc gcagcgcgcc tcgccgccag
     1981 cctgaaggcc gactggatcg ccgtctacgt ggaaacgccc gcgctgcagc gcttgcccga
     2041 tgcccgccgc gaacgcacgc tgaacgcctt gaagcttgcc gccgaactcg gcgccgaaac
     2101 cgcgacgctc gcggccaccg atgcggtcgc cgccctgatc ggttacgcgc aggcacgcaa
     2161 cgtctccaag ctggtggccg gcgcgtcggc ccgcaccggt ttcatgcgct ggctgcgccg
     2221 gccgctcggc gagcgcatcg ccgaacgcgc gggcgatctc gatctcacgc tgattcgcgc
     2281 ctcgtcggaa cgtgacggcg gcgagcatcg gcgcttgctg aacaccactg ccaacgcatg
     2341 gcgcgaggtg ttgagcacgg cgcgcgaacg ccgctcgccg ccgcgcgcct acggtatcgc
     2401 gctggcgatt tgcgcggcca tcacgctgct gtcgagccag ttgatcgacc gtatcgatct
     2461 ggccaacctc gtcatgctgt acctgctcgg cgtgatcttt accgccgtga agctgggccg
     2521 cgggccgggc gtcgtcctgt cgttcatgag cgtggccgcg ttcgatttct tcttcgtgcc
     2581 gccgcgcatg tcgctgtcgg tgtccgatac gcagtatctg ctgaccttct tcggcatgct
     2641 gctgacttcg ctcgtcatca gccatctcac ctcgagcttg cgccgcgagg cgagtgtcgc
     2701 gcagcgccgc gagcaacgca ccggcgcgat gtacgccatg gcgcgtgaac tcgcggcggc
     2761 gctcaccacc gagcagatcg tcggcatcgg cagccggcat gtgagcgaag tgttcggcgc
     2821 gcgtgtggcg atcctgttgc cggacagcgc cgatcaggtg aagcagaaga tcgaagaccc
     2881 cgacgcggcc atcacgctcg aaggcgaact gctcgatagc gacgtcggcc aatgggtcta
     2941 cgatcagcag aagcctgccg ggcaaggcac cgacacgctg ccggccgcgg ccgcgcggta
     3001 cttgccgctg aaggcgccga tgcgcacgcg cggcgtgctg gcggtcgtga tgcgcgatga
     3061 acgtgagctg gacgtgcccg agcagcaacg catgctcgat gcgttcgccg cgcaaatcgc
     3121 actggctctc gagcgtgtgc actacgtcga catcgcgcgc gacgcgctcg tcaatatgga
     3181 atcggagcgc ttgcgcaatt cgctgctgtc ggccatctcg cacgatttgc gtacgccgtt
     3241 gacgacgatc gtcggcttct cgtcgatgct ggcgcaatcg cgtgaggcgc atcccggtgc
     3301 gcaacccggc gacgatctcg tcgaagcgat ccacgaagaa gcgctgcgca tgaccggcat
     3361 cgtcaccaat ctgctcgaca tggcgcgctt gcaggcaggc agcctgcaat tgaaccggca
     3421 atggacgctg ctcgaagaaa ccgtgggcgc cgcgctgcgg gcatgcaaac gcgtgctggc
     3481 gaatcatccg gtgcaggtga agctgcccgc ggatttgccg ctgctgcatc tggatgccgt
     3541 gctgatggag cgcctcttct cgaatctgtt cgagaatgcc gctaaataca ccgcaccgga
     3601 cacgcctttg acgatcagcg cgcaaggcct cgacgcggac ggcaagtcgt tcgtgcgcgt
     3661 cgcggtcgac gataccgggc cgggtttgcc ggccggcatg gaagcgcgtg tgttcgagaa
     3721 attcacgcgc ggcgagaagg agtcggccaa gccgggcatc ggcctcggcc tcgcgatctg
     3781 ccgcgcgatc gtcgaagcgc acggcggtac aatcggcgcg ctcaatcgct tatccgcgga
     3841 cggccgcgtc gaaggcgcgc gcttctggtt cacgctgccg gtggaaacgc cgcccgaagc
     3901 acccgatgcg ctggaagacg aaggcgccga cgcgttgccc gatctcgatt caactcttat
     3961 ccagcattcc aagccaagcc atgagtgacc cgagcatcac agtcgtcctg attgaagacg
     4021 aaaagcagat tcgccgcttc gtgcgaacgg cgctggaagg cgagggcatc gtcgtgcacg
     4081 acgccgagac cggcaagcaa ggtctcgtcg aagccgcgac gcgcaaaccc gatctcatca
     4141 tcgtcgatct cgggctgccc gacaccgacg gtctcgatgt gattcgtgaa ctgcgtggct
     4201 ggagcgaact gccggtgatc gtgctgtcgg cgcgcaccca ggaaaccgag aaagtggcgg
     4261 cgctcgacgc cggagcggac gattacctga ccaagccgtt cggtgtctcc gaactgctgg
     4321 cgcgcatccg cgcgcatatg cggcggcgca atcagggcgg cgcgaatgaa tcgccggtgg
     4381 tgcgcttcgg cgcggtgacg gtcgatctgg ccttgcgcca ggtgagccgc gacggcgttg
     4441 ccgtccatct gacgccgatc gaataccgcc tgctcgcgac gctggtgcgt cacgccggcc
     4501 gtgtgctgac gcaccggcaa ttgctgcgcg aggtgtgggg gccgtcgcat gtcgagagcc
     4561 atcactatct gcgtatctac atggcgcacc tgcgcgggaa actggagcgc gatccggcgc
     4621 aaccggagca tatcgtgacg gaaaccggcg tgggataccg gctggtggga gcggtttagc
     4681 gttgcccgag gcgcttgtcg ggtttgcaag tggggttcgt gcacgagttg ctcgcaagcc
     4741 aggcggctcg tgctcgctct cgcttgcgag atgcccgctg agccttgcgg ctggtcgccg
     4801 cagcggcgcc gcgcaccatt ttggtacaat tgcagatcgt aaggacgacc ttacgcatcc
     4861 gctacgaacg gggacggccc catctgacca tggagctgtc tcgtgtcctg gtttcttctg
     4921 ttgattgccg gtttgctcga agtcgcgtgg gcggccggtc tcaaaacctc cgaaggtttc
     4981 acccgggtct ggccctccgt attcacggtg gtaaccgcgc tcggcagctt tgtcctgctc
     5041 gcaatggcga tgcgccagtt gccgcttggt acggcctatg ccgtgtggac ggggatcggt
     5101 gcggtcggcg cgttcatctt cggcatcgtc atgatgggcg aggccctgac gcccgcgcgg
     5161 gtggcaagcg cggcgctgat cgtgatcggg ttgatcgggt tgaagttgtc atcagggcat
     5221 tgagcccttg atattcagac ggagtagcga tgtgcgagtg tgagtgaagt gcgagtgtgc
     5281 tcgcgctgcg cgccgactga acgccaatca catgccaacc ggagccaacc ggacgcgaac
     5341 cggagccaac cggcgtcaat tgggcgccaa ttcggcgcca gccagacgtc tttggcgccc
     5401 gccatttgtt cgccgctctg cgttctgcga attaacgtgg ctataatgag attgaatcca
     5461 acctgaaatt gcccgcggcc catttggcgc ggccggcttg gcgcgggtct gacgacttcg
     5521 gtgcggcggt tccctttccc ttgcccgtcg aagcgcccgg cacgcacagc gcgcctggag
     5581 gcggccatgc ttgaatcact ttcgcttgcg gcgggcctct catggggcag cggcctgcgc
     5641 ctctatctga cagtcctgct ggccggcgtg ttcgcgcgtc tgggcctcgt ccatctaccc
     5701 gatacgctgt ccgccctgtc ttcgccgtgg gtcatcggtg tggccggcgt gctgacgatc
     5761 acggaatttc ttgccgacaa aatccccgcg ttcgattcgt tgtgggacgc gatccatacg
     5821 tttatccgca ttccggccgg cgctatgctg gcggccggcg cgctcggtca tgccgatcct
     5881 gcgctgctga cggtcgcggc gctcgccggc ggcacgctgg caggcaccgc gcatctggcg
     5941 aaagcgggca cacgcgcgct gatcaatctg tcgccggaac cgctctcgaa tgtcgtgacc
     6001 tcgaccgccg aagacggtct cgtgttcggc ggcatgctgc tcgcgctgtt tgtgccgctc
     6061 ctgtttctgg tgttgatggt gggctttctg atgctggcga gttgggcgtt accgcggctg
     6121 tggcgcgggg tgcaaggcgg ttttcgaggg atggcgacgc acatggtgtc gcgttttgcc
     6181 aggagcaggc acgattgagc gatacggtac ggagtacgac ggcgcgcgct gattcaaagc
     6241 cgacgcgccg gctgggcggc atggacgtgc tgcgccaggc catccgcatg acggcgcgcg
     6301 attggcgcgc cggcgaactg acgatgctgc tgttggcgct ggtgctggcc gtcgcggctt
     6361 tgtcgagtgt cggctttctg gctgaccgct tgcaccaggg tcttgagcgt gatgcgcggc
     6421 gcatgatcgc cgctgacttc attgtccgcg ccgatcatcc ggtcgatccg cagttcgcgc
     6481 agcaggcgaa agcgctcggt ctgaacaccg ccagcacggc aatcttcccc agcatgatca
     6541 actcgaccgg cgcgcagccg gtgtcgcggc tcgccgccat caaggcggtg tcgccgggtt
     6601 atccgttgcg tggcgcgttg cggattgccg cggcgccggg cgcggcggac cacgccaccc
     6661 gatcgattcc ggcgccgggt acggtgtggg tcgatcaggc actgctcgac gccttgaagc
     6721 tgcatgtcgg cgatgctgtg aaggtcggca ctcgcagctt ttcgattggc gcgatcatca
     6781 cccgtgaact cgaccgtggc ttctcgttcg tcaatttttc accgcgtttg atgctgcgtg
     6841 ccgacgatgt ccaatcgacc ggcctcatca ccttcggcag ccgcgtgacg taccggttgc
     6901 tggtggcggg cacggatgca tcggtggcgc gcttcgcgca attcgcgcat gacagggtgg
     6961 acggcggcaa gatgcgcggc gtcgcgctcg aatcgttgca agacggtcag ccgcaagtgc
     7021 gtcagacgct cgaccgggcg agccacttcc tcaccctggt ctcgttgctc accgccttgc
     7081 tcgcggcggt cgcgatcgcg atggccgcgc atcggtacat gcgccggcat ctggatggct
     7141 gcgcggcgat gcgttgcctc ggtgtcagtc agcggacgct gcgcacgctg ttcacgctgg
     7201 aattcgtcgc gctgggtttg atcggcggcg tgctcggcgt ggcgctcggt tacctggggc
     7261 acatggcgtt gctgacgtgg ctcggcagtc tgatcgacgt cgccttgccg tacccaagcg
     7321 tgtggcccgc gctcgaaggg atcgcggccg gcctcgtctt gttgctcggc ttcgcgttgc
     7381 cgccgctgtt gccgctgacg cgcgtaccgc cggtgcgtgt gctgcgtcgc gaatggggtg
     7441 aagcggggcg caccgcctgg gccgcctatg cgctcggcat cgcgctgttt gccgcgctcc
     7501 tgatcgtcgc ggcgggcgag ttgaagcttg gcggcattgt cgcgggcggc ttcgccggcg
     7561 gactgctggt gttcgcgtgc atcgcgcggg ccgcgctgtg gggcgcggcg cggctcgtgc
     7621 gcagcgagcg cgtgaacgcg ggaatcggct ggcgttatgc gttggcgtcg ctggagcggc
     7681 gcagcggagc gagcgcgttg cagatcaccg cgctcggcat cggcctgatg tgcctcttgc
     7741 tgatcgcgat gacgcgtgac gacctcgtgg caggctggcg caaatcgaca ccgcccgacg
     7801 cgccgaatga attcatcatc gacatccagc ccgaccagcg cgacgccgtc acgcagtatc
     7861 tgaacacgca tggcgtgccc ggcacggtgc tctcgccgat ggtgcgcggc cgcttgatcg
     7921 ccatcaacgg caagccggtc aatccggatt cgttcaaggg cgcagatgcg aggcgcctcg
     7981 tcgaccgcga atttaatctg tcgtacacga ccgagctgcc cgaggacaac cgtctgtctg
     8041 ccggctcgtg gtacggcgac acgaagacgc cgcagatttc gatcgagcag gggcttgcga
     8101 agctgatcaa tgtgaagccc ggcgacacgc tgcgcttcga cgtgacgggc ttgcagatcg
     8161 atgcgccggt gacgagtgtg cgcaagctcg actggggctc gttcaaggtc aactttttcg
     8221 tgctgatgcc gccggccgcc ttgcaggatt tcccggcgac gttcatcacg agcttccatt
     8281 tgccgcccga aaaacaatcg accatcgacg ggctgatcgc tgcctatccg aacctcacgg
     8341 cgatcgacac cggtccgatt ctcgcgcagg tgcagcgcgt gttgcagcag gtgatcggcg
     8401 cggtgcagtt cctgttcgcg ttcacgctgg cggccggcgt gctggtgctc tatgccgcgc
     8461 ttgccggcac gcgtgatgag cggatgcgag aatccacgct gctgcgcgcg ctgggggcct
     8521 cgcatcgcca ggtgcgggcg gtgcaagttg ccgagttcgt gtcggtgggc gcgctggcgg
     8581 gtttgatggc ggcggttggc gcgcaggtga tcggctggtt gctggcgacg cgcgtgttcg
     8641 agttttatct gacgttcgat ccgtggttgt tgcccgcggg tgtcgcggct ggcattgcct
     8701 gcgcgggcgt gggcggctgg ctgagcttgc ggcatgtgct ggcgcggccc gcgttgcaat
     8761 cgctgcggga cgcgtgaatt ttctttcctg ttttttgtga ttgggtatga gcgatcttga
     8821 cgacgaagtg tccgcggcac agccgacggc cttcgaactg gtgggcggcg aaacgcgtgt
     8881 gcgcgagctg atcgaccggt tttatgacct gatggatctc gagccggaat tcgccgggat
     8941 tcgggccttg catccggagt cgctcgacgg ctcgcgcgac aagtttttct ggttcctgtg
     9001 cggttggatg ggtggacccg atcactacat cagccgcttc ggccatccga gattgagggc
     9061 acggcatatg ccgtttccga tcgcttccag cgagcgcgat cagtggctcc gttgcatggc
     9121 gtgggcgatg gaagacgttg ggctttccga gccgttgagg gagcggttgc tggcgtcgtt
     9181 cttcgagacc gcggactgga tgcgcaaccg caatggttga tggggcagcg gggctggaag
     9241 cagaggcgcg cgcagtgctc gactgctggt tcggcgctcc ggattcgtct accttcggcc
     9301 atgcgcggaa gtgctggttt tcccgcgaca aagccttcga tgcgatgctg ctggaacgct
     9361 tcggaacgct aatcgacgcg gcgcgcgaag ggtctctcga cggctggatg gcaacatcgc
     9421 tcggcgcgtt ggcgctggtc atcgtgctgg atcagttttc gcgcaattgc catcgccata
     9481 caccgcgcgc tttcgctgcc gatcatcagg cgctgcgcat cgcacagcgg atgatcgcaa
     9541 gcggcgccga tcttttgttg ccgggcgcgc atcatcgcgc gttcgcgtat ttgccgttcg
     9601 aacacgacga aacgctcgtc agccagcacg aatcgctgcg cctgttcgaa cagctgaagg
     9661 ccgagccggg cggcgcgtcc tactactcct ccgcgctcag gcacgccaga atcatcgagc
     9721 gtttcggacg ctttccgcat cgcaatgcgc agctcgggcg tcagtcgacg gttgaagaaa
     9781 cggcgttttt gaaagaacct cgctcgtcgt tttaggcgcg tggcgaacgc gtaaggcgaa
     9841 cacgcgagcc gaacgcgtaa gccgaacacg taagcggaac gcgcaaggcg aacgcgcaag
     9901 gcgaacacgc aagccgaaca cgcaagccga acgcgcaagg cgaacacgca agccgaacac
     9961 gcaagccgaa cacgcaagcc gaacgcgcaa ggcgaacacg caagccgaac acgcaacgca
    10021 aacacgcaac gcaaacacgc agcattggtc acgcgctatc tggcgcgacg tgctactcgc
    10081 cgcgcgcggg cggcgccggc gtccggacga acacgcgcaa cacttcgtcg gtgtcacccg
    10141 gccgggcgcc cgaccagaac aattgccaat cgcctgccgg cgtacgtgcc gtgtcgcggc
    10201 ggctctcttc aatcagccac gtgcactcgg tcgtcgcggt gttcgcggtc ggttgatgtt
    10261 ggatgccggt gtagtagtac agcatcggcg cctcggattc gccgagtccg gcgctcgcca
    10321 tgcaatcgcc atcgttccat tcgatattca tctgcgcacc gaggttctcg aacaccgagc
    10381 ggtagctctt cgcatagtcg agccacggca gcagcagtgt gctcaccagt ccccagacga
    10441 gaatcgcgcc cgcgcaccag ctcagcgcgc cgcgccattt gccggtgtat ttgaagaccg
    10501 gcagcaacca tagccagcag agcgtcatta gcagcgcggc tgcgatcagc ccgggcttga
    10561 ccggcagcag ccagtcgagt ggcaaccagc ggccgagccg gcgcagcgac gcatgcgtgt
    10621 tggccggatc gctcatgatc gaccagatga tccacgcgag cgcggccgcg ctgccgaaca
    10681 gcacacggct cgcgatgtcc cagcttgcgt gcagacgccg cggcagcttt tcgatgcctt
    10741 gcattgccac cagcgcgagc ggggcgatga acggcagaat gtacagttcg cgcacggtgg
    10801 ccgagctttg cagtaccgcg aaacccgcgc cggcgaagat caccggcagc gccacgcgcg
    10861 gatcgcgcca gcggcgccac ccgccgccgg caagcgcggc cagcgccagc ggtacgaccg
    10921 gaaagccgac gctcagcaca gtgcgcagca cgaacagacg gctctcgttt tcagagccga
    10981 gctcggccac cgaaaagccg aagaaacggc cgacgttgtt gtcccagagc cagaccttga
    11041 agagcgcttc agagcgcaga tagaaacaga tcggccagat cagcgcgaag ggtgaagaga
    11101 cgagcgcggc gatgccgagc gccccggcaa aactgcgcgt gcggcaggcg ggatacagca
    11161 ccagcacggc gacggtggtc acgccgaaca cgagcggcac gaacaggccc ttcgccagca
    11221 acgatacccc tacgcctgcg ccgaacatcg ccgcgccgct gaaaatcgcc tggcggcgac
    11281 ggcgtgcgtc gaccggcagc aggcgtgctt cgtcgcgcag atgcaccagt acgagttcga
    11341 acaggccgca gaagccaagc gcggcgccgg ccattagcgc aacgtcggtc atcatgtcgt
    11401 gcacatgctt gacgaccaca agcgtgccgg cgaacagtgc aagcgtgccg atcacgcgga
    11461 tgtcgaacca gctcgacgcg gtcaccgcgc ggcgggcggc gcgtgccgtg tagtagacgg
    11521 ttagtccggc gaagagcgca ctcgcgaggc gtgcggcgtc atgcaaaggc aggtagcggc
    11581 cgaacatcca cgcgaggccc gctgcgaccc agtcgtagat cggcggcttt tcgacaaagg
    11641 gctggccggc gttggtcggc acgaccagat cgcccgtttc gagcatgtgc tggacgatgc
    11701 cgaacgtgta ggtctcgtcc tgcttccatg gctcgtggcc caggatgccc ggcaggagcc
    11761 aggcggacag gattgccagg gcggccagcc acatcagcgg acggacggcc gcgagcgcgc
    11821 gcaagcgttg agcgccacgc gaagcgggag ctaaagcggt gggttccgtg ggaccggcgg
    11881 gggcggccgg ctttgcgggc gccgcggctt tgactgcgtg agcggctgac aacgcggcgc
    11941 cggattcggc ggcgtgtgcc acggcggggc gcgtgtccgg cgcagcgtcg gcggggccgg
    12001 cgggcgttgg cgtcgcaatc gcgacgttcc ccgtgcgcca gggcgcggat gacttttcgt
    12061 gcatgagatt ctttggtcgc cgggcgcggc gatgtgggcc gcgagcttat gcaatggctg
    12121 tgtgccgcat gcggagtgca tgcgaatgct gcgattgtag tctcggttta ttacggccga
    12181 ttacgggaaa cgtcggaaat gttgcgctca gccgaacggc ttgcgccgat acctcgtcaa
    12241 ttcgaaggga atcgggaatt ggaggtaggg aatcgagaag ggaggatcgg gaaggaagag
    12301 gcggctggtg ggaaaccgct gaccccgcaa acagcagaag ggcggctcag cagcagaagg
    12361 gctgttcagc ggcagagcgc ggctcagcgg cagagggcct cagcggcaga gcgcctcagc
    12421 aagaagagcg gctcagcagc agaagaccgg cgcagcagca gaagaccggc tcagtagcag
    12481 agcacctcag tagcagagcc cctcagcagc agagcccctc agcagcagag cacctcagca
    12541 acagaacggc cgctcagcgt ccgccgctga cgtcgagcag cgcgcccgtc acatacgagg
    12601 cggcgtcgct cagcaaccag acgatcgact cggcgacttc gtcggcccgg cccgggcggc
    12661 ccagcggtgt ggtcgcgcca agttgggcgg cgcgctcggg cttgccgccg ctggcgtgga
    12721 tctcggtatc gatcaggcca ggacgcactg cgttcacgcg cacaccctgc gggccgagtt
    12781 ccttggcgag gccgatggtc atggtatcga cggcgccttt cgagccggcg tagtcgacgt
    12841 attcgtttgg cgaaccgagg cgcgaggccg ccgacgagag gttcacgatc acgccacccg
    12901 ccccgccacg ggtggtcgac atccggcgcg cggcttcgcg tgcacacaga tatgcgccga
    12961 gcacgttgac gtcgaacatg cgcttcagac gcgcgaggtc catatccgcg agctggctcg
    13021 acggcgcgac gatcccggca ttgttgacga gcgcatcgag ccggccgaac ttctgctgga
    13081 gggcgtcgaa catcgcgatc acgtcggctt cgctggcgac gtcgccggca atcggcaacg
    13141 cgtggccgcc gacgcgttcc acttcagcaa cggtttcctg cgcggccgcg agattcgtcg
    13201 cgtaattcac gccgaccgac cagccgcgtg cgcccagcaa acgtgctgtc gcgcggccaa
    13261 tgccgcggct gccgccggtg atcagaacgg ttttcgtcat cgcaatctca actttcggga
    13321 ggagccgcag gcgcgtgcgg ccggggttca ttcacatgcc tgaacaggca atcgaacagg
    13381 cgcggcagac gacaatcaaa cggcgtcgcg cttgatcgcc catttgtcgt tggcaggcgg
    13441 ctggtagcgt tgcagcttgt cgatcagcgc gacaggatcg gcctccattt gcaggatgtc
    13501 gaagtaggtc tgccgcatga agccttcttc taccgtgtgc ttgagcagcg cgatcagcgg
    13561 atcgtaaaag ccgtcgatat tcagaacggc taccggcttc tggtgataac cgagttgtgc
    13621 ccacgtatag acctcgaaca attcttcgag cgtgcccacg ccgcctggca tcgcgacgaa
    13681 cgcgtcggac agatcggcca tcatcttctt gcgctgatgc atgtcgggca ccacatgcaa
    13741 ctcggtcagc ccgttatggc cgacttcctt gttgaccagc aactcgggaa tcacgccgat
    13801 tgcgcggccg ccttcggcca tcaccgtatc ggcgatcacg cccatcaggc cgaccttgcc
    13861 gccgccatag accagcgcga gatcggcggc taccaacgcg cggccgaagg cgcgcgcggc
    13921 ttcggcgtac aagggtttgg ctccgtcgga ggagccgcaa tacacacaca ccgacttcat
    13981 gctcaagtgt ccttcggttc gttacggggc acggcgcctt ctttcggcgg caggtaatcg
    14041 tagaactcgc gtttgggcag cttgcccgag acgaggtcgt cgaaaatctg ccgtgagcgg
    14101 ccgcgcagat aaggcgccat cagcgagacg atctgcacgc tcacctgatg cagttcagcg
    14161 cgaatggttt cctgctcgtt gtacttgcgc gggttcatca cgaactgata cgacagccag
    14221 tacgtcgcga tcacgccgat gttagtcgcg atcacttgca gctcttcggg cgtggcgacc
    14281 atctcgccgt ccgccaccag ttgctcgcag aactggctcg caaagcgcac cttgtggctg
    14341 atgatctgct tgaagtgcgt ttccagtgtg cggttgcgtg ccagcagatc gttcagatcg
    14401 cgatacagga aacgatagcg ccaggtgaaa tcgaccatgt actgcaggta cgaccacatc
    14461 tcgtcgatgg tcgcgcggtg atcgtcggga aagcgcagac gtttttcgat ctcctgctcg
    14521 aactggccga agatgctgtt gatgatgtcg tctttgttgc ggaagtggta gtacaggttg
    14581 cctggactga tttccatttc ctcggcgatc gtcgttgtcg tgacgttcgg ttcgccgatc
    14641 tcgttgaaca gcttcaacga tagttcgaga atccgttcgc gggtgcggcg gggaggtttg
    14701 gcttccatgt agtccggccc tggttacgcc gggcttgcat gatccacccg gacgcggttg
    14761 gtatattgag actatcgatc gattataaac cgaccgttct tataccaaga aaatactcag
    14821 ggttttcatt gctgcgggcg cgacggcagg acgcgccctg ggattcatag ccacgctttg
    14881 acccaacgca cgacgagcgg cccgacaatc agcccccagg tcatcagaca catgccgagc
    14941 gcgaccatga ccgccgcgct gccgagatcc ttggcgcggc gcgacaactc atggcgttcg
    15001 agcgaaatcc tgtcgatggc cgcctcgacg ctcgaattca gcaactcaac gatcagcacc
    15061 agcagcaccg agccgagcag cagcacgcgc gacacggggt cgaccggcac cagcacgcca
    15121 cagggaatca gaatcgcagc gagcgtgagt tcctggcgga acgcgctttc ctcgcggatc
    15181 gccacccgaa aacccgctag cgaatttttc atcgcgtgcc atgcgcgggt caggccgcga
    15241 ttgcccttgt atggattgaa agggaggggg gcgagcggat cgtcggggct gagcggctcg
    15301 tggtgcaagt cgctatcggg gctgtcggag cgctcgaggc ttgcgccatt catgccacgc
    15361 aaaccattcg cgtgattggc atgatttggc gcagcgtgcc cagtatggcg ctcagtgtcg
    15421 tgctcagggt cgtgctcacg cacgtcgtcg aacggctcga tgccggcggt gtcagtgccg
    15481 gcatcgacag cgcgcatcgc gccgttcgac gcgtgtgccg cggtggagtt tcgggttcgc
    15541 atgagcggct cggcgcgccg cctcatgcgg cggcgctttc ctcactgtac gaggcgcgcg
    15601 gcagcggctt cagatgcgcc gcgaactgct cggacgccgc agcccacgag aagcgttcgg
    15661 cccatgcgcg tgcatggctg cgatcgatct tgagtgcttc gaggcaggcc tcgcgcaggt
    15721 cttcgtgcat ggcgcccgca ccgccgtcgc cgagcacgtc gatcgggccg gtcaccggat
    15781 acgccgcgac cggcgtgccg caggccagcg cttcgagcag cacgagcccg aaggtatcgg
    15841 tgcggctcgg gaacacgaat acgtcggcgg cggcatagac cttcgccagt tcggcctgcg
    15901 tcagcacgcc caggtaattc gcttgcggat agcgtgactt cagttcggcc agcgccgggc
    15961 cttcgccggc aacccacttc gagccgggca ggtcgagctt cagaaacgcc tcgacgtttt
    16021 tctcgacggc cacgcgtccc acgtacagga agatgggccg cgcggtgttg aggaccttcg
    16081 agtccatctg gtgaaagata tcgagatcga cgccgcgggt ccacagcacc acgttggtga
    16141 agccgaattt ttcgaggtcg gtcttgacca ctggtgtggg cgccatcacc gccagcgacg
    16201 ccttgtggaa ccagtgcaga aacttgtagg tggccgcgag cggaatgccg aaacgcgcct
    16261 gcacatattc gggaaaacgc gtgtgatagg cggtcgtgaa cggcagcttg tgctggatcg
    16321 cataggcccg cgcggccatg ccgagcgggc cttcggtcgc gatatgcagc gcatcgggcg
    16381 cgaattcgcc gatacgctgg cgcagcttgc ggcgcggcag cagcgacagg cgaatctccg
    16441 gataggtcgg gcacggaatc gtcttgaatt cgagcggcgt caacaagtcg acgcggtggc
    16501 cgagcgcggt caattcgcgc gtggtgtttt tcagcgtacg cacgacgccg ttgacctgcg
    16561 gttcccatgc gtcggtgacg atcatgatct tcatcggttt cggcccaata gaagtggaga
    16621 gggactacgc ggtcgcccgg gccttttgca cgccgacttc cggggcgcgc atcacggtcc
    16681 agtagatcac cttgagttca ccttcgtacg tctcgacgag tgccgacagg ctttcgaccc
    16741 aatcgccgtc gttgcaatac agcacgccgt cgatgtcgcg gatctcggct ttgtggatgt
    16801 gtccgcaaac cacgccgtcg cagccgcggc ggcgcgcttc gtcggtcatc acgcgttcga
    16861 acgaggagat gaaattcacc gcgttcttca cctgatgctt caggtattgc gacagcgacc
    16921 agtaggggaa gccgaggcgg ctgcggatcc ggttgaacca gcggttcagg atcaggatca
    16981 tcgtgtagag cgtgtcgccc agatacgcga gccatttggc atgctggatc acgccgtcga
    17041 acagatcgcc gtgcacgatc cacaggcgct tgccggcgag cgtcgtgtgg aatgcttcgc
    17101 cgcgcacgtg gatgtcgccg aacgcgaggt cgcagaactg gcgcgcggct tcatcgtgat
    17161 tgcccggcac gtaaatgacc tgcgtgcctt tgcgcgcttt gcgcagcacc ttctgcacga
    17221 catcgttgtg cgcctgcggc cagtaccagc cttttttcaa ctgccagccg tcgatgatgt
    17281 cgccgacgag gtacagatat tccgactcgt tgtgccgcag aaagtcgagc agatagggcg
    17341 cctgacaacc actcgagccg agatggatgt cggatagcca gatcgtgcga tagcggtgcg
    17401 gttcggcgtg ctcgtcgtcg tggtgaccgg cgtgcgcgtc gagcggcagc gacggtacgg
    17461 gcaacggcag tccgtgacca ggatcgggtt ccgggcggaa cgcgacaggg tcgacgctcg
    17521 ggccggtctg gcggaacagg gaggtcgcgg acgttttggg gtccatggct cacgcgtcga
    17581 gttgcggtgc ccgtattgcg ccagccgcgc gtgaccgtgc cgtgacagtc acgagaagtt
    17641 cttattactg tgtgggagag gaaagctacg ttaggggagc ggcggagcgc gcagaacggc
    17701 ggtgtgaaag gagaccggga aagggcgtga cgccgcaaaa atggcgcagt gatgcgacga
    17761 gaggcacgcg caggcaagcg caggcaagcg caggcgaccg cagaaaagcg aaagcgaatc
    17821 gcggacgcgg cggcgtcgat tacagggcgg tcagtttgcc tggcgtacga cgctgaccac
    17881 ccgtgtaccc gcgaggcggt catgcaggaa ttgacgctgc gaatcgaagc ggccggtggc
    17941 ggcccacagc acgaaccaga tcgcggcgat caccagcgtc tgcggcacgg cgaggccgag
    18001 caacggatgc agcgcgagcg gcggcaaaaa ccacagccag gccaacacgt aacgaacgat
    18061 cgcgcggccg gtggacagcg gggcgccgtc agcggcgatg acgcgcaggt gccaggtttt
    18121 catcggcagc gtttggccgc tgtgcgtcca gaaccagacg aaatacagac cgaccacgag
    18181 gccgatccac gctgcgagca ggttatggtg ggtgaggccg ttgcgctgct gcgtcaacgt
    18241 gctgaacaga taagccgcga cgaacacgac gccgaacagg atcacggctt cgtagagcag
    18301 cgcggcgagg cgccggcgaa cggtgggagc ggtgccggat gaagcggcgg gcagggtcga
    18361 agtggcgagc ggttgggaca cagtgggccg gatcaggagc cgtggaagat cgacggcgcc
    18421 tcggacggcg acaccggcac cggcgtggcg ggcacgaagc tgcccgcagc cggaatcgcc
    18481 ggcgtggaag ccgggctcgg caatggcacg gcgagcgggg cactgaccgc cgcggcaccg
    18541 ctcgcgccgt gctcgcgggc attgaccaga cggtccaagc ccttgatctc cgacttgccg
    18601 gacggcggcg cgctgaccac gctcgggcgc cgcttgcgct cgctcgcggc gagccgctcc
    18661 ttctgttctt ccggcaattg ctgataagcc ttccacgcat tctggcgggt ttcgcgcggc
    18721 agctccttcg acacctgata gttttcgcgt gcaacacgcc gctgatcggg tgtcatacgc
    18781 gtccattcag tcattcgatc atgtaggcgt ttttgcgcat cgggcgacaa cttcgcataa
    18841 cgcgatgcaa ccttgagcca ttttcgcttg cgttcttcgc tgaaggagtc ccattggctg
    18901 gcgaacggcg cgagcgcaac gtgctcggcc gtgctcaggc gcgaccatgc gagtgggcta
    18961 ttgctggtgg gcagactcgg cagttcgacg gcgagcgtgg gcgccttggc ggtcgcattg
    19021 ccgcttgcgg caggcgcgct gacgggcgat gggctcggat aaaagcgcgg atacgtcgcg
    19081 gcaaacgaca ccagggccgc gatcgtgcat ccgaaaacaa cggccaagcc gcgcttgtaa
    19141 ctcacccgaa ggatctcccg ctcagtgggc gcgtgataga tacgcgttga aaccgtggtc
    19201 gagataggca ttgagcggca agtcgtcgct tagcatggct gcatcgatat cggcgagttc
    19261 ggcagtgcgt tgctggtctt cccagtaagc gatgcccacg aggctgacga cgagcgcgac
    19321 cagcggccag gcgagcgcaa agcggcgcag cggcgaacgg cggcgctgcg gcaggtcgac
    19381 ttgcggcata ccggccggca tgccggcacc ggcgaaagcg ggcacgaaca ccggcgcgct
    19441 cacggtttcg ggcttcttgc gagcgagcgc ggcgcggcgc gccgccgcga gccggtcgac
    19501 ggtggcggcc ggaatgctgg cggcggtttc gtcgagcgcg cggcgcacct ggcgcgcgaa
    19561 ctcgagttct ttggtttctg gggagctcat agcgtgattc ctttagcctt gagcgcttgc
    19621 gccagcgtgt gggtggcccg cgagcagtgc gttttcacgc taccctcgga gcagcccatt
    19681 gcggcggcag tctcggcgac atccatatct tcccaataac gcatgagaaa cgcctcccgt
    19741 tgacgtgccg gtaacttttg gatctcgtcg tcgattaact gtaagacctg ttcgcgttcg
    19801 agtttctgtt cgttgctctc cgagcccgct gaaccttgct gcgcctcgaa ggtttcgagc
    19861 gggtcgaact cgtcgtcatc ggcgttgccg agcgaggaaa acaggctaac ccaagtattg
    19921 cgcactttcg cacgacggaa atagtcatgc atcgcattct ggagaatacg ctgaaacaga
    19981 agcggcagtt cggcgggcgg acggtcgccg tatttttcgg cgagcttgat catcgcgtcc
    20041 tgcacgatat cgagcgaggc gtcgtcgtcc cgcacggcgt agaccgtctg cttgaatgcg
    20101 cgcctttcga cgcccgccag aaaatcggcg agttccttgt ctgatgccat ccgtttgggg
    20161 gtcggcgcag cg
//
LOCUS       DATASH010000131         3293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4963255,
            whole genome shotgun sequence.
ACCESSION   DATASH010000131 DATASH010000000
VERSION     DATASH010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4963255"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1163)
                     /locus_tag="VHV99_03955"
     CDS             complement(<1..1163)
                     /locus_tag="VHV99_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HEX3634635.1"
                     /translation="MTADERDAVFALLDDCGATAARRSSRLYTGFVQERVCADAAQLE
                     AVCETVAVDTLRGLHAVVLADYEFGRRLLDSGLPHRVSKETQRGDATLRFLLFERCEK
                     LSRDQVDAWLVERDGGAPEASVAGTANVRESVDPKQFNEAIQAIHAALRAGDSYQVNF
                     TYRLSFDVFGSPTALYRRLRARQPVSYGALIALPDDEWVLSCSPELFIDKEGAALRAR
                     PMKGTAPRSADPVADRHAAEFLANDPKNRAENVMIVDLLRNDLSRVAQTGSVNVPALF
                     SVEPYASVWQMTSTVHSTLRARTSFAEIMRALFPCGSITGAPKHRTMQLIDELESTPR
                     GLYTGAIGWLDAPAVAAEAATASSGNTAVGVGITTAPATATAAAAAPATATATA"
     gene            complement(1188..2330)
                     /gene="dnaJ"
                     /locus_tag="VHV99_03960"
     CDS             complement(1188..2330)
                     /gene="dnaJ"
                     /locus_tag="VHV99_03960"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006417099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX3634636.1"
                     /translation="MAKRDYYECLGVAKNASDDEIKKAYRKLAMKHHPDRNPGNKDAE
                     GHFKEVKEAYEMLSDSQKRAAYDQYGHAGVDPNMGGAGAQGFGGFADAFGDIFGDIFG
                     QAAGGAARGGGGRAGPQVYRGADLRYSMEITLEQAAHGYDTQIRVPSWVSCEICHGSG
                     AKPGTKPETCPTCSGSGQVRMSQGFFSIQQTCPKCHGTGTYIPEPCGHCHGAGKVKET
                     KTLEVKIPAGIDDGMRIRSAGNGEPGINGGPSGDLYVEIHIKQHSVFERDGDDLHCQM
                     PIPFTTAALGGEIEVPTLAGRASFTVPEGTQSGKTFRLRGKGIKGLRSSIAGDLYVHV
                     QVETPVKLTEPQRDLLKQFEKALVEGGARHSPQSKSWFDRVKSFFD"
     gene            complement(2600..>3293)
                     /locus_tag="VHV99_03965"
     CDS             complement(2600..>3293)
                     /locus_tag="VHV99_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX3634637.1"
                     /translation="HAQVYSTADDNQGAVTIKVFQGEREMAAGNKLLGEFNLEGIPPA
                     PRGTPQIEVSFDIDANGILHVGAKDKATGKENRITIKANSGLSEAEIEKMVKDAEANA
                     EEDHKLRELADARNQGDALVHSTKKALTEYGDKLDAGEKEAIETALKDLEETLKSGSS
                     DKAAIEAKIEVVATASQKMGEKMYADMQAAQGAEAAAAGAAGAGAGASAGGASQQQDD
                     VVDAEFKEVKKD"
BASE COUNT          552 a         1108 c          945 g          688 t
ORIGIN      
        1 gctgtggctg tggctgtggc tggggctgcg gctgcggctg tggctgtggc tggggctgtg
       61 gtgatgccga cgccaacagc agtgttacca ctggaagcgg tggcggcttc tgcagcaacc
      121 gcgggcgcat cgagccagcc gatcgcgccg gtataaagac cccgcggcgt gctttcgagc
      181 tcgtcgatca attgcattgt gcgatgctta ggcgcaccgg tgatcgagcc gcatggaaac
      241 agcgcgcgca tgatctcagc gaaagacgtg cgggcgcgca gcgtcgaatg caccgtcgac
      301 gtcatctgcc acaccgacgc atacggctcg accgaaaaca gcgccggtac attgaccgag
      361 cccgtctgcg ccacgcgcga caaatcattg cgcaacaggt cgacgatcat cacgttctcg
      421 gcgcgattct tcgggtcgtt tgcaaggaac tcagcggcat gacgatcggc cacgggatcg
      481 gctgatcgcg gcgccgtgcc cttcatcggc cgggcgcgca acgcggcacc ctctttgtcg
      541 atgaacaatt cgggcgagca cgacagcacc cattcgtcgt ccggcagcgc gatcagcgca
      601 ccgtacgaca ccggctgacg cgcccgcagg cgtcgataaa gcgcggtggg cgaaccgaat
      661 acatcgaaac ttagccgata cgtgaagttg acctgatagg aatcgcccgc gcgcagggcg
      721 gcatggatcg cctggatcgc ctcgttgaat tgctttggat cgacgctctc gcgcacgttt
      781 gccgtgcccg ccaccgacgc ctcgggcgcg ccgccgtcgc gttccacgag ccacgcatcc
      841 acctggtcgc gcgacagttt ctcgcaacgt tcaaacaata aaaaacgcag cgtggcatcg
      901 ccacgctgcg tttctttcga tacccggtga ggaaggccgc tgtccagaag tctccggcca
      961 aactcgtagt cggcgagcac gacggcatgc aaaccccgca gcgtgtccac ggccacggtc
     1021 tcgcacacgg cttcgagttg cgcagcgtcc gcgcaaaccc gttcctgcac aaagcccgtg
     1081 tacagacgac tcgaacggcg cgccgccgtc gcgccgcaat cgtcgagcaa cgcgaacact
     1141 gcgtcgcgtt catctgccgt catgcttacc gccaaatcaa accgctgtta atcgaagaag
     1201 ctcttcaccc gatcgaacca gctcttgctc tgcgggctat gccgcgcgcc accctcgacc
     1261 agcgcctttt cgaattgctt gagcagatcg cgctgcggct cggtgagctt gaccggcgtt
     1321 tcgacttgca cgtgcacata cagatcgccg gcaatgctcg aacgcagccc cttgatgccc
     1381 ttgccacgca gacggaacgt cttgcccgac tgcgtgcctt ccggcaccgt gaagctcgcg
     1441 cggcccgcga gggtcggcac ttcgatttcg ccgccaagtg ccgccgtggt gaacggaatc
     1501 ggcatctggc aatgcaggtc gtcgccgtcg cgttcgaaca ccgagtgctg cttgatatga
     1561 atctcgacgt acagatcgcc cgacggaccg ccattgatac ccggttcacc attgccggcc
     1621 gagcggatgc gcatgccgtc gtcgatacct gccgggatct tcacttccag cgtcttggtt
     1681 tccttcacct tgcccgcgcc gtggcagtgg ccgcacggtt caggaatata ggtgccggtg
     1741 ccgtggcact tcgggcaggt ttgctggatg ctgaagaaac cctgcgacat ccgcacctga
     1801 cccgaaccgc tacaggtcgg gcaggtttcc ggcttggtgc cgggcttcgc gcccgaaccg
     1861 tgacagattt cgcacgacac ccagctcggc acgcgaattt gcgtgtcgta accgtgtgcg
     1921 gcctgttcca gtgtgatttc catgctgtag cgcagatccg cgccgcgata cacctgcggg
     1981 cctgcacgac cgccaccgcc gcgtgccgca ccgccggccg cctggccgaa gatatcgccg
     2041 aaaatatcgc caaacgcatc ggcaaatccg ccgaagcctt gcgcaccggc tccgcccatg
     2101 ttcggatcga cgcccgcatg gccgtactga tcgtacgcgg cgcgcttttg cgagtccgac
     2161 agcatttcat aggcttcctt cacctctttg aaatgccctt ccgcatcctt gttgcccgga
     2221 tttcggtccg ggtgatgctt catcgcaagc ttgcgataag ccttcttgat ttcgtcgtcg
     2281 ctcgcgttct ttgcgacgcc caggcactcg tagtaatccc gtttcgccat atcggttcaa
     2341 cgccactcgc gcaatggggc gcggtggctc ctcttgaatg ctggagtctt gcgactcgtc
     2401 cggccgctgt tttccgccgc tttcacgcga ctcaaaacaa cgccctccat aaaacaaatg
     2461 tgcccggaga gcccaaacag gctcaccagg cgcgataacc gctttgcaga ttcatgcgcc
     2521 cggacgggag caaaaccgtt ttgcagccaa gccgcacaca tcgctgtgcg cggctgtacg
     2581 gtcaaactgc gacctggctt cagtctttct tcacttcctt gaactcggcg tcgaccacgt
     2641 cgtcctgctg ctggctcgcg ccaccggccg aagcacctgc acctgcacct gccgcgcctg
     2701 ctgccgcagc ttcggcacct tgcgcagcct gcatgtcggc gtacatcttt tcgcccatct
     2761 tctgcgaggc cgtggccacc acttcgatct tggcttcgat cgcggccttg tcgctcgaac
     2821 cgctcttcag cgtttcttcg aggtccttca gcgcggtttc gatcgcttcc ttctcgccgg
     2881 cgtccagctt gtcgccgtac tcggtgagcg ccttcttcgt gctgtggacc agcgcgtcgc
     2941 cctggttgcg ggcatcagcc agttcacgca gtttgtgatc ttcttccgcg ttcgcttcgg
     3001 cgtccttcac catcttttcg atttcggctt cggacagacc cgagttcgcc ttgatcgtga
     3061 tgcggttttc cttgccggtc gccttgtctt tcgcgccgac gtgcagaatg ccgtttgcgt
     3121 cgatgtcgaa gctcacttcg atctgcggcg taccgcgcgg tgccggcgga atgccttcaa
     3181 ggttgaactc gcccagcagc ttgttgcccg ctgccatttc gcgttcgccc tggaacacct
     3241 tgatcgtcac ggcgccctga ttgtcgtcag ccgtcgagta gacctgtgcg tgc
//
LOCUS       DATASH010000132         1798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3578238,
            whole genome shotgun sequence.
ACCESSION   DATASH010000132 DATASH010000000
VERSION     DATASH010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3578238"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /locus_tag="VHV99_03970"
     CDS             complement(<1..956)
                     /locus_tag="VHV99_03970"
                     /inference="COORDINATES: protein motif:HMM:NF014728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX3634638.1"
                     /translation="MLEAAHTLRAANPACSVVVGYIEPHGRVETERLLSGLELLPPLA
                     VKYRGIVRKEFDLDAALERRPSILLVDELAHSNVLGGEPAPRHPKRWQDIEELLDAGI
                     TVWTTVNVQHLESLNDLVAQITGIRQRETLPDRIFDEADEIELIDLPPDDLLARLHAG
                     KVYVAGEVATAVERFFRTPNLMALRELALRRVAARVEAAARALPSNRGRGRFAGDRIL
                     VAVGPDEQAEQLVRAGKRMADALDAGWTVVYVETPALLRLSEAERDRRIDVLRLAESL
                     GAEAVTLDGPSAAATLIEYAKTRNATRVVVGSPKRKPWWAWF"
     gene            complement(1087..1671)
                     /gene="kdpC"
                     /locus_tag="VHV99_03975"
     CDS             complement(1087..1671)
                     /gene="kdpC"
                     /locus_tag="VHV99_03975"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HEX3634639.1"
                     /translation="MNTIIRPAVTLFLIMTVLTGIVYPFAVTGLAQVLFHDQAEGSLV
                     MANGHAVGSRIIGQSFSDPKYFWSRPSATAPQPYNAIASTASNLGPLNPALTDAVGTR
                     IAALKAADPTNNLPVPVDLVTASASGLDPDISIAAARYQATRVARIRGLDLAAVQSLI
                     DAHARGKLFGLIGEPRVNVLELNLALDALRAPNK"
BASE COUNT          327 a          517 c          626 g          328 t
ORIGIN      
        1 tggaaccagg cccaccaagg cttgcgtttc ggagatccga cgaccacgcg ggtggcattg
       61 cgcgtctttg cgtattcaat gagggtggcg gcagccgatg ggccatcgag cgtcaccgcc
      121 tcggcaccca aggactcggc gagccgtagt acatcgattc ttcgatcacg ctcggcttct
      181 gacaaacgca gcagggctgg tgtttcgaca tacacaacag tccatcctgc atcgagtgcg
      241 tcggccatgc gcttgcctgc gcgcacgagt tgctctgcct gctcatccgg cccgacagcg
      301 acgaggatgc ggtcaccggc gaagcggccg cggccccgat ttgaaggcaa tgcccgggcc
      361 gccgcctcaa cacgcgcggc aacccggcgc aatgcgagct cgcgcagggc catgaggttg
      421 ggcgttcgaa agaagcgctc aacggccgtt gccacttcac cggcgacgta gaccttgccg
      481 gcatggagtc gggcgagcag gtcgtccggg ggcaggtcga tcagctcaat ttcgtcggct
      541 tcgtcgaaga tgcggtcggg cagggtttcg cgctggcgaa tgccggtgat ctgggcaacg
      601 aggtcgttca ggctttccag gtgttgaacg ttgactgttg tccagacggt gatgccggcg
      661 tccagcagtt cttcgatgtc ctgccagcgc ttgggatgac gcggcgccgg ctcaccgccc
      721 aagacgtttg aatgcgcgag ctcatcgacc agcaggatgc tgggacggcg ctcgagcgcg
      781 gcatcgagat cgaactcctt tcgcacgatg ccgcgatatt tgacggccag gggaggcaac
      841 agctcgaggc cgctgagcag ccgttcggtt tcaacgcggc cgtgtggctc gatataaccg
      901 acgactacgc tgcaggcagg gtttgcggca cgcagggtgt gcgccgcttc gagcatcgcg
      961 aatgttttgc cgacgccggc ggacgcgcca aagaaaatgc gcaggcggcc gcgcttggcg
     1021 cgggcttctt cggcctgtac gtgagcgaga agctggtcgg ggtcaggtcg ggggaagtcg
     1081 ggtgagttac ttattcgggg ctctcagtgc gtccaacgcc aggttcagct ccaggacgtt
     1141 gacgcggggc tcgcctatga gaccgaacaa cttaccgcgc gcgtgggcat cgatcaatga
     1201 ttgtacggct gccaggtcga gtccccggat acgggcgacg cgagttgcct ggtagcgtgc
     1261 agctgcaatg ctgatatcgg ggtcgaggcc gctggccgat gccgtcacga gatcgaccgg
     1321 tacgggcaga ttgttggtgg gatcggctgc tttgagcgcc gcaatgcgag tccctacggc
     1381 atccgtcaac gcgggattga gggggcccag gttggaggcg gtggaggcaa tcgcgttgta
     1441 cggctgcggt gcggtggccg atggccggct ccagaagtac ttgggatcgc tgaagctctg
     1501 gccgatgatg cgcgagccca ctgcatgtcc gttggccatg accaggctgc cctcggcctg
     1561 gtcgtggaac aggacctgcg caagtccggt tacggcgaag ggatacacga tcccggtcag
     1621 cacggtcatg atgaggaaca gggttacagc cggacggatg attgtattca tgggaaacct
     1681 caaaccaggt gtgtgagcac gaggccgacg tcaatcagct tgatgcccgc aaagggaatg
     1741 atgatgccgc ccaggccgta gatcagcaga ttgcggcgga gcaggacggc ggcgccca
//
LOCUS       DATASH010000133         5742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1007871,
            whole genome shotgun sequence.
ACCESSION   DATASH010000133 DATASH010000000
VERSION     DATASH010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5742
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007871"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="VHV99_03980"
     CDS             complement(<1..699)
                     /locus_tag="VHV99_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEX3634640.1"
                     /translation="MSVLIADWKKYICRACGVIYDEELGDPDSGIAAGTRFDAIPDDW
                     ECPLCGVTKTDFVLYEPVVAGAAACAPARGASSRTPGVIVVGGGTAGWAAVEAVRALD
                     PDIPITLVSACSADRYRKPELSISLSEKRTPERLVFETGPAAATRLGVRLVAHTYAIG
                     LSPALHQLRTTAGTFRYRQLILAQGARPVVPSGLPRELCWRVNDLSMWSSMQRRLTAG
                     PARVAVIGAGLIGCE"
     gene            complement(696..1838)
                     /locus_tag="VHV99_03985"
     CDS             complement(696..1838)
                     /locus_tag="VHV99_03985"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX3634641.1"
                     /translation="MEHALVAEESGVDSWCDEAVLARVAAIARGPLAAAAPRIDGEGV
                     YPLDIMGELGAAGALGVHLDRYGARFGLSLAAMQEASRACGSTGFLMWAHDVCGLYME
                     QSGNPYLGGARLDAHARGQTFGGTALSNPMKAFSGIEPMMFRATPTPGGYLINGTLPW
                     VSHIGLGQYCGAIAAVHRDDGSVSHEVMFLLSCDARVELRHCPVFSGMEGTSTWGVKL
                     SNYFVGEADLIADPARPFVARIKAAFILLQVGMALGVAQGGIDSCLEVEPVLGHVNRF
                     LHDRPDELTAELDEAVSAMQRLARTAYDGSTDYLLEVLDLRTRGAELALRAAQASLLH
                     QGARGYLMSAAPQRRIRESHFVAIVTPAIKHLRWEMARLSAEELPA"
     gene            complement(1904..2458)
                     /locus_tag="VHV99_03990"
     CDS             complement(1904..2458)
                     /locus_tag="VHV99_03990"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX3634642.1"
                     /translation="MSTVTDNPTTISTYLRPIDRDGLTAFAEKGRNNPGSRGKNNVHT
                     VMQGQYRSLSYVGNHTPVVVDEPLHLFGEDTAPAPGEIALSGLGGCLAVGITAVATWK
                     QIKLSKLEVFLEADIGNPAAWGAGGAEMEPAQMGFQAIRVKIVIEGDATREELDEIVR
                     HANYFSPVANSMRNPIPMTVALAD"
     gene            complement(2710..4362)
                     /locus_tag="VHV99_03995"
     CDS             complement(2710..4362)
                     /locus_tag="VHV99_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX3634643.1"
                     /translation="MQTRSDASADSESAGYWRQHEMLLLQQVMALVGKSLSPAPALRE
                     MLHLMSELLGLNRGRIVLADETRRSGRIRYAYGLTPEEISRGVYAYGEGITGRALQHG
                     QLTIIQDIDREPDFLSRAVERSRLPDGPVSFIALPIVVERRTVGVLACHRIRHRSRML
                     ADDITVLRILATLTGQLLQLEAGLEEKTRALEEQNAVLARALESAAARYGIVGTSPPL
                     LRAISELERVSDSAASVLLLGESGTGKELFARALHLASSRRDRAFIKINCAAIPDTLF
                     ESELFGYERGAFTGANSMRAGWFEQASKGTLFLDEIGELPLMMQAKLLRTLQEGTITR
                     LGGKAEIHVDARLVAATNCDLRRGVAQGTFRQDLYYRLNVIPIHLPSLAERHQDIRVL
                     ALHFISHTNQLNQRNVNLTAAALDRLEEYSWPGNIRELTNFIERVVLLADKPLLDAAD
                     IGWLLPSGAADEPAVAPASAQWAGAVRGPGGALDASSHDPVSHSGPLPMRPYRRVGSH
                     PAQALVDALEQCGGNKSRAAQRLGMTPRQFSYRWQKLGLPND"
     gene            complement(4503..5663)
                     /locus_tag="VHV99_04000"
     CDS             complement(4503..5663)
                     /locus_tag="VHV99_04000"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX3634644.1"
                     /translation="MSALPGVAFDNSVEAEREILFRVVNRVAIITLNRPAALNALSHA
                     MVRELAVLVEHCRTDDGIVALVLKGAGTKGFCAGGDVREVYRLAKNGDSRWLAFFVDE
                     YRLDYALHTFPKPVVALLDGISMGGGMGLGQAARLRIVTERSKIAMPETRIGFLPDVG
                     ATRFLSVMPAQVELYVGLTGVTLSGADALRLQLADLCVPAEWLVSFEERLQRMSHAGD
                     LMAALRGVFEPPCNVIPHAALGPFTQLILRHFDRRSSVERIVATLRHDLEREPPREVK
                     QWLQATYDAMVGHSPTMLYVAREALLRGRQMSLAECFRMELGVVKRVIEEGDFCEGVR
                     AQLIDRDRRCRWAPATLAEVRPERVRYFLASPWKRETHPLVGLGGEEGKAGW"
BASE COUNT         1050 a         1968 c         1763 g          961 t
ORIGIN      
        1 ctcgcagccg atcagccccg cgccgatcac cgctacgcgc gccggccccg ccgtcagccg
       61 gcgctgcatg ctgctccaca tcgacagatc gttgacgcgc cagcacagct cgcgcggcaa
      121 gccggacggc accacgggcc gagccccctg ggcgaggatc agttgccggt aacggaacgt
      181 gcccgccgtc gtgcgcagtt gatgcagcgc cggcgacaac ccgatcgcgt aggtatgggc
      241 gaccagccgt acgccgaggc gcgttgcggc ggccggcccg gtctcgaata cgagccgctc
      301 cggtgtgcgt ttctcgctca acgaaatcga cagttccggc ttgcggtaac ggtccgccga
      361 gcaggcgctc accagcgtga tcgggatgtc cggatccagc gcccggactg cttcgacggc
      421 tgcccaaccc gccgtgccgc cgccgaccac gattacccca ggggtgcgcg acgacgcgcc
      481 ccgcgccggc gcgcacgccg ctgcgccggc cacaaccggc tcgtaaagca cgaaatccgt
      541 ttttgttacc ccgcataacg ggcactccca gtcgtcgggg atcgcgtcga agcgcgtgcc
      601 ggccgcaatg ccgctatccg ggtcccccag ttcttcgtca tagatcacgc cgcacgcgcg
      661 gcagatgtac ttcttccagt cggcaatcag gacgctcatg ctggcagctc ctcggcggaa
      721 aggcgggcca tttcccaacg cagatgcttg atggcgggcg tcacgatcgc cacgaagtgc
      781 gactcgcgaa tccgccgctg cggcgctgcg ctcatcaaat agccgcgcgc gccctgatgc
      841 agcagcgacg cctgcgctgc acgcaacgcg agctccgcgc cgcgtgtgcg cagatcgagc
      901 acttccagca gatagtcggt gctgccgtcg taggcggtgc gcgcaagtcg ctgcatcgcg
      961 ctcaccgctt cgtcgagttc ggccgtcagt tcgtctggac ggtcgtgcag gaagcggttc
     1021 acgtgaccga gtaccggctc gacttcgaga cacgaatcga taccgccttg tgcaacgccc
     1081 agcgccatgc cgacctgcag caggatgaac gccgccttga tccgcgcgac gaaaggccgc
     1141 gccggatcgg caatcaggtc ggcttcgccg acgaaatagt tgctgagctt gacgccccac
     1201 gtgctggtgc cttccatgcc ggagaacacc gggcagtggc gcaactcgac gcgcgcgtcg
     1261 cagctcagca ggaacatcac ctcgtgtgag actgaaccgt catcgcgatg caccgcggcg
     1321 atcgcgccgc aatactggcc gaggccgatg tgactgaccc acggcagcgt gccgttgatc
     1381 agatagccgc caggcgtcgg cgtggcgcgg aacatcatcg gctcgatgcc tgagaacgct
     1441 ttcatcggat tcgacagcgc ggtgccgccg aacgtctggc cgcgggcatg cgcgtcgagc
     1501 cgcgcgccgc ctagatacgg gttgccggac tgttccatgt acaacccgca gacatcgtgg
     1561 gcccacatca ggaagccggt cgagccacac gcgcggctcg cttcctgcat cgcggcgagc
     1621 gacagtccga agcgcgcgcc gtagcggtcg aggtgaacgc cgagcgcgcc ggccgcaccc
     1681 agctcgccca tgatgtcgag cgggtagacc ccttcgccgt cgatccgtgg cgcggctgcc
     1741 gcgagcggac cgcgagcgat cgcggccacg cgcgccagca ccgcttcgtc acaccagctg
     1801 tcgacgccgg actcttctgc taccaatgcg tgttccatgt tggtttcctt cgggtacctg
     1861 gggcagccgc tacgcgcggc gcgcacgagc gagagcggcg ctctcagtcg gcgagcgcga
     1921 cagtcatggg aatcggattg cgcattgagt ttgcaaccgg cgagaagtag ttagcatgac
     1981 ggacgatctc atcgagctcc tcgcgcgtcg catcgccttc gatcacgatc ttcacgcgga
     2041 tcgcctggaa acccatctgc gcgggttcca tctcggcgcc acccgcgccc catgcagccg
     2101 gattgccgat atccgcttcg aggaacactt cgagcttcga cagcttgatc tgcttccacg
     2161 tagcgaccgc tgtgatgccg accgcgaggc agccgccgag accggacagc gcgatctcgc
     2221 ccggcgccgg cgccgtgtct tcgccgaaca gatgcagcgg ctcatcgacc acgacgggcg
     2281 tatggttgcc gacatagctc aacgagcggt actgaccttg catgaccgtg tgcacgttgt
     2341 tcttgccgcg cgaccccggg ttattgcgac ccttttcggc gaaggccgtc aacccatcgc
     2401 gatcgatcgg tcgcaggtag gtggaaatag tcgtgggatt atcggtgaca gtgctcaagt
     2461 gacgctcctg gtgcgtggat ggtgaaggtg cgggcgtcgc atcgaaggtg gagcggcgcc
     2521 ggcacatcac tcaatgcaac aggcgtgcca gttacactgc gtatcgccaa actgctttgc
     2581 tggcggggct ttgggccaga caagaagtgc caccagggtt cggcgcgaca cattcttaat
     2641 caagcggtga accgacattg tcgacaatgt cgacaatgtt tactctgtga tgcgcgggcg
     2701 gcctggccgt cagtcattcg gcagaccaag tttttgccag cgatagctga actggcgcgg
     2761 tgtcatgccc aaccgttgcg ccgcgcgcga tttgtttccg ccgcactgtt cgagcgcgtc
     2821 gacaagcgcc tgcgccgggt gcgaaccgac acggcggtaa ggccgcatcg gcagcggccc
     2881 actgtggctc acaggatcat gcgatgaagc gtccagcgct ccgcccggtc cacgaacggc
     2941 gcccgcccat tgcgcagacg cgggcgcaac agcaggttca tccgccgcgc ccgacggcag
     3001 cagccagcca atatccgctg catcgagcag cggcttatcc gccagcaaca cgacccgctc
     3061 gatgaaattg gtcagctcgc ggatattgcc gggccacgaa tactcttcga gacgatccag
     3121 cgcggccgcg gtgagattga cgttgcgctg gttgagttga ttcgtatgac tgatgaaatg
     3181 cagcgcgaga acgcgaatgt cctggtggcg ttcagcgagc gacggcaggt gaatcggaat
     3241 cacattgagc cggtagtaca ggtcctggcg gaaggtgccc tgcgccacac cgcgtcgcaa
     3301 atcgcaattg gtggccgcga cgaggcgcgc gtcgacgtgg atctccgcct tgccaccgag
     3361 acgcgtaatg gtcccttcct gcagcgtgcg cagtagcttg gcctgcatca tcagcggcag
     3421 ctcgccgatc tcgtcgagaa acagcgtccc tttgctggcc tgctcgaacc agcccgcgcg
     3481 catgctgttc gcgcccgtga aggcgccgcg ttcgtatccg aacagctcgg actcgaacag
     3541 cgtatccggt atcgccgcgc agttgatctt gatgaaagca cggtcgcgcc gcgaactcgc
     3601 cagatgcaag gctcttgcaa acagctcctt gccggtgcct gactcgccga gcaacagcac
     3661 gcttgcggcg gaatccgata cgcgctccag ttccgagatc gcgcgcagca aaggcggcga
     3721 ggtgccgacg attccgtagc gggcggccgc cgattcgaga gcccgcgcga gcactgcgtt
     3781 ctgctcttcg agcgcgcggg ttttttcctc gaggccggct tccagctgca gcaactgccc
     3841 cgtcagcgtc gcgagaatgc gcagcacggt gatgtcgtcg gccagcatgc gcgagcggtg
     3901 gcgaatgcgg tgacacgcaa gaacgccgac cgtccgccgt tcgacgacga tcggcagcgc
     3961 gatgaacgag accgggccgt ccggcaaccg tgagcgctca accgcgcgcg acagaaagtc
     4021 cggttcgcgg tcgatgtcct ggatgatcgt cagttggccg tgctgcagcg cgcgcccagt
     4081 aatgccttcg ccgtacgcat acacgccccg gctgatttcc tcaggggtca gtccgtacgc
     4141 gtaccggatg cgcccgcttc gacgcgtttc gtccgcgagc acgatgcggc cccggttcag
     4201 gccgagcagc tcggacatca gatgcagcat ctcgcgcagc gcaggcgcgg gggacaggct
     4261 tttgccgaca agcgccatca cctgctgcaa tagcagcatt tcgtgttggc gccagtagcc
     4321 ggcggattcg ctgtcggctg aggcgtcaga tcgtgtttgc atgggcgtag accgactcgg
     4381 tcgtgagcga ggcctattat ttcagaacac aaaaaagtcc gcagccacgc tgcccgatcc
     4441 agcgtcaacg cacgacgccg cgcgtccggt tcaggttgca ccacccttcg cagagactgt
     4501 cgctaccaac cggccttccc ttcctcgccc cccaatccaa ctagcggatg cgtctccctc
     4561 ttccacggcg aagccaaaaa ataccgaacc ctttccggcc tgacttcagc gagcgtcgcc
     4621 ggcgcccagc gacatcgacg gtccctgtcg atcaactgcg ccctgacacc ctcgcaaaag
     4681 tcgccttcct cgatcacccg cttgacgacc cccaactcca tccggaagca ctcggccaaa
     4741 ctcatctgcc gaccacgcaa cagcgcctcg cgcgcaacgt acaacatcgt gggcgaatgc
     4801 ccaaccatcg catcgtaagt agcctgcagc cactgtttaa cctcgcgcgg cggctcacgc
     4861 tccagatcat gacgcaacgt cgccacaatc cgctcgacac tcgaacgccg gtcaaaatgc
     4921 cgcaggatca actgggtaaa aggccccaac gcagcatgcg gaatgacatt gcacggcggc
     4981 tcgaacaccc cgcgcaatgc ggccatcaag tcccccgcgt gcgacatgcg ctgcaggcgc
     5041 tcttcgaaag agacaagcca ctcggccggc acgcacagat cggccagttg caggcgcagc
     5101 gcatcagcgc cggataaggt cacgccggtc agccccacat acagctcgac ctgcgccggc
     5161 atcacgctca gaaaacgcgt cgcgccgacg tccggcagaa agccgatccg agtctccggc
     5221 atcgcgatct tgctgcgttc ggtgacgatg cgcagccgcg ccgcctggcc gagccccatg
     5281 ccgccgccca tcgagatccc gtcgagcaac gcgaccaccg gcttgggaaa cgtgtgcagc
     5341 gcatagtcga gccggtattc gtcgacaaag aacgccagcc accggctgtc gccattcttc
     5401 gcgagccggt acacctcgcg cacgtcgccg ccggcgcaaa aacccttggt gcccgcgcct
     5461 ttcagcacga gggcgacgat gccgtcgtcg gtgcggcaat gttcgaccag cacggcaagt
     5521 tcgcgcacca tcgcatgcga tagcgcattg agcgcggctg gccggttcag cgtgatgatc
     5581 gccacccggt tgacgacgcg aaacagaatc tcgcgctccg cttcaacgct gttatcgaac
     5641 gcgacgcctg gcaacgcgct catcgatgag tctcctgagg gacgtttgca acctgctcag
     5701 cgtgcgctgc atgctccgcc tgtccagctt gcgtcacctg cc
//
LOCUS       DATASH010000134         4025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6149747,
            whole genome shotgun sequence.
ACCESSION   DATASH010000134 DATASH010000000
VERSION     DATASH010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6149747"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VHV99_04005"
     CDS             complement(<1..195)
                     /locus_tag="VHV99_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010095942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX3634645.1"
                     /translation="MTRTNLRSISIAPDHNKSQLSKGQKAFNTLIKQIEKRRARLSAW
                     EAAMPAFHRKYLSEFAPLEQT"
     gene            complement(370..1986)
                     /locus_tag="VHV99_04010"
     CDS             complement(370..1986)
                     /locus_tag="VHV99_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014989244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEX3634646.1"
                     /translation="MNSGARPPSPEDDLKHLSPDALIARILELQSQNQQLAQRIGQLE
                     ELFRLAQLKRFAPSSEKLKDRVFDEAEQIAATEPTDDDPGDALALPDTGLPEVDKPER
                     GKRGRKPLPAELPRRRIEYDLPDEQKICPCCQSALHRMGEQMSEQLHIEVKASVLQHV
                     RLKYACRHCEQHAEHTPIVTAPMPAQPLPGSNASAAMIATVTTGKYVDGTPLYRMEHA
                     LARAGIPVGRGTLANWIIRPAELHYRRLYEAMRKTLLSQPLIHGDETTVQVLKEEGKS
                     AQSKSYMWTYRSAQDSEQPVVLFDYQPGRGQQYPQAFLAGYDGMLMTDGYSAWRTLEG
                     VTHFGCMAHSRRLFVDAHKGQKKKAGGRALQALEYFKALYQVETLARAELPVGQTRAA
                     YAYRLRQQHSVPLLEALKTWLDTQAPQVLPESLLGKAISYTRNQWEYLSRYVIDGRAP
                     VDNNILERDIRPFATARHSWLFSDTVAGAKASAMVYSLVLTCRACNVEPYAYLHHVLT
                     EMPQRTSDADISDLLPFNFAKRVQLATTHP"
     gene            complement(2092..2445)
                     /gene="tnpB"
                     /locus_tag="VHV99_04015"
     CDS             complement(2092..2445)
                     /gene="tnpB"
                     /locus_tag="VHV99_04015"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEX3634647.1"
                     /translation="MFRLDAELRVYLHRDAVDFRNGINGLVILVEQSMQLDPFARAVF
                     AFRNKRANRIKLLFYERSGFWLMIKRLEADRFVWPRRQQAVMELTTEQLHWLLEGIDI
                     DAVRRHPSRQYRHVS"
     gene            complement(2772..3734)
                     /locus_tag="VHV99_04020"
     CDS             complement(2772..3734)
                     /locus_tag="VHV99_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX3634648.1"
                     /translation="MNDIKLSASGEAVFGPLVSHGRFPYRPITDTDNFRWPGDTGLAV
                     YLGFNIEHFAFGEGLGAALGPVSPEPDVLNYSWREYGNRVGVWRCLELFDQLEMPAGM
                     LINTSLFDHCPEVIDACVARGDEIIGHGHTNSQRQSDLDEGGERALLLHCRERIANHA
                     GVPPSGWLSPWISESHLTPDLLAETGYSYTLNWCHDDRPVRMKTRNGSIWSIPYPQEL
                     NDIPMMVARRLDGRAFADMIIDQFDEMLEQVKSTRHPQSLVMGIALHPYLVGQPYRLR
                     HLRRALQHIAAARTRGEVWLTTPGAIARHMDAVESAAQVKAAAR"
BASE COUNT          763 a         1206 c         1210 g          846 t
ORIGIN      
        1 agtttgctca aggggagcga actcgcttag gtatttccga tggaaggcag gcattgcagc
       61 ctcccaggca ctcagcctgg cgcgccgctt ttcgatctgc ttgatcagcg tgttgaacgc
      121 cttttgcccc ttcgacagtt gagacttgtt atggtcggga gcaatgctga tggacctgag
      181 atttgtcctg gtcatgggat gatttccgct ggttgatgcg agagcgagca cccagctatt
      241 ttagccgagg tcgcggttgc aacgcgctgg cgtccgcgcc gcgatagcga gcacgcgcac
      301 gttgccgacc aatgatcgac ttatgccgga gctcgtggag atgcggactg gaccccagat
      361 cggggtgctt caggggtggg ttgtagcgag ctgcactcgt ttggcgaagt taaacggcag
      421 caggtcgctg atgtccgcgt ccgatgtccg ttgtggcatt tcggtcagca catgatgcag
      481 atacgcgtag ggctcgacgt tgcaggcccg gcacgtcagc acgaggctgt agaccatggc
      541 actcgccttc gcgcccgcaa ctgtgtcaga gaagagccac gaatgtctcg ctgttgcaaa
      601 tggcctgatg tctctttcca gaatgttgtt atccacgggc gcacgcccat caatcacata
      661 gcggcttaga tattcccatt ggtttctcgt gtagctgatc gccttgccca acaggctctc
      721 aggcagaacc tggggcgcct gcgtatccag ccatgtcttg agcgcttcta gcaacggcac
      781 gctgtgttgc tgccgcagcc ggtacgcgta ggcggcccga gtctggccaa cgggcagttc
      841 tgcccgagca agcgtctcga cctgatagag cgccttgaag tactcaagcg cctgcagcgc
      901 gcgaccaccg gctttcttct tctgcccctt gtgcgcgtcg acgaacaggc gccgcgaatg
      961 ggccatgcat ccgaagtggg tcacgccttc gagcgtgcgc caggcgctgt agccgtcggt
     1021 catcagcatg ccgtcgtagc cggcgagaaa cgcctgcgga tattgctgcc cgcgtcccgg
     1081 ctggtaatcg aacagcacca ccggctgttc gctgtcctgt gcgctgcggt acgtccacat
     1141 gtacgatttg ctttgcgcgc tcttgccttc ttccttcaga acctgcaccg tcgtctcgtc
     1201 gccgtgaatc agcggctgcg acaacagtgt cttgcgcatc gcttcgtaca gccggcggta
     1261 gtgcagctcg gcaggcctga tgatccagtt cgccagcgtg ccgcggccca ccggaatgcc
     1321 cgcacgggcc agcgcatgtt ccatccggta cagcggcgtg ccgtcaacat atttgccggt
     1381 cgtcactgtc gcgatcatgg ccgcgcttgc gttgctgccc ggcagcggct gggccggcat
     1441 cggtgcggtc acgatcggcg tgtgctccgc gtgctgctcg cagtggcggc acgcgtactt
     1501 aaggcgtacg tgttgcaaca ccgacgcctt gacctcgatg tgcaactgct cgctcatttg
     1561 ctcgcccatg cggtgcagcg cgctctgaca gcacgggcaa atcttctgct cgtcgggcag
     1621 gtcgtactcg atgcgccggc gcggcagctc cgctggcaac ggcttgcgtc cccgtttgcc
     1681 acgctcgggc ttatcaacct ccggcagtcc cgtatcgggc aatgctaatg catcacccgg
     1741 atcatcgtcg gtgggttcgg tcgcagcgat ctgctcggct tcatcgaaca cgcgatcctt
     1801 gagtttctcg ctgctcgggg cgaagcgctt gagctgcgcc agacggaaca gttcctcgag
     1861 ttgcccgatg cgttgcgcaa gttgctggtt ctgggactga agctcgagaa tgcgcgcgat
     1921 cagcgcatcg ggcgagaggt gtttgaggtc gtcctcaggg gacgggggac gggcgccgct
     1981 attcatgtcc tccacgatag cgaagccgca caacgaatgg gtttacgtca aagtgtaaca
     2041 attgcgccgg gcaccatgcg taccacgccc ggcggcgctg ctactgaacg ttcagctcac
     2101 gtgccggtac tgtctcgaag ggtgacgacg taccgcatcg atgtcgatgc cttcgagtaa
     2161 ccagtgaagc tgctcggtcg tgagctccat caccgcctgc tgtcggcgtg gccagacaaa
     2221 acggtctgcc tctaatcgtt ttatcatcag ccagaaaccc gagcgctcgt aaaacagcag
     2281 cttgatgcgg ttcgcacgct tgttacggaa ggcgaagacc gcacgggcaa atgggtcaag
     2341 ctgcatggac tgctcgacca ggataacgag accgttaatc ccgttcctaa aatcgaccgc
     2401 gtcgcggtgc aggtagaccc gcagctcagc gtccagccgg aacacggtag cgccccagcg
     2461 tttcgatcat cgccgacacc aacgtcgtgt cctgcccact ccgggggagg tcgagttcac
     2521 ggtgcagggg taactcccca tcctccggcg cgcattgcac agtgcgctgt ggcgccgggg
     2581 gaggttccgc actgcaataa gtgatgcggc ctcgcgtaca cgcaacgcct catttatacg
     2641 ggactgtcgt ccttcgccag ttgcccttaa tgcctcgatg agatcggatg gtgacatcca
     2701 aatctaccca acagagcatg catcgacctt gatagtgccg tcaagcgagc gcatgaatgt
     2761 tcggagagcg cctaccgcgc cgccgccttc acttgtgccg cgctttccac cgcgtccata
     2821 tgccgcgcga tcgcaccggg cgtcgtcagc cagacctctc ctctcgtccg ggctgctgcg
     2881 atgtgctgca gtgcgcgccg caagtggcgc aaccggtacg gttgccccac gagataagga
     2941 tgaagcgcga tgcccatcac caaggattgc ggatgacggg tggacttcac ctgttcaagc
     3001 atctcatcga attgatcgat gatcatgtcg gcgaatgcgc gaccatcgag ccgacgcgcc
     3061 accatcattg gtatgtcgtt gagttcctgt ggatagggga tcgaccagat cgagccattg
     3121 cgcgttttca ttcgcaccgg gcggtcgtcg tgacaccaat tgagcgtata gctgtagccg
     3181 gtttcggcga gcaagtccgg cgtgagatgc gactccgaga tccatggcga gagccagcca
     3241 gagggcggta caccggcgtg attggcgatg cgttcgcggc aatgcagcaa caacgcgcgc
     3301 tccccgccct cgtcgagatc gctttgccgt tgcgagttcg tatggccgtg cccgatgatt
     3361 tcgtcgccgc gcgcaacgca tgcatcgatg acttccgggc agtggtcgaa cagcgacgta
     3421 ttgatcagca tgcccgcggg catctccaac tgatcgaaga gttcgaggca acgccagacg
     3481 cctacacgat tgccgtattc gcgccagctg tagttgagta cgtcaggttc gggcgaaaca
     3541 ggcccgagcg cagcgccaag tccttcgccg aatgcgaagt gctcgatatt gaagccgaga
     3601 tagacggcga gcccggtgtc gcctggccag cgaaagttgt ccgtatcggt gatgggacga
     3661 tagggaaagc gtccatgcga gacaagcgga ccaaagacag cttcgcctga agctgagagt
     3721 tttatatcgt tcatgtgcaa agacttctga gacgtaggtt acggcaccgc ggatagtgcg
     3781 ggtgatcatg cgcatcttgc aaaaatcgcg tcgacaatac gccaatttct tgtatgcatc
     3841 aagacaagat gtggtgcacc aaatgtgatc ctgatattcc cgaaaactag gcaagagcgc
     3901 gccaattggg ggcgcgacag ccttacgact tgtgcagatc gcggaaccgc atcctccagc
     3961 aacgatcacg tatacgtcga ttcttcatcg cgcatccgac tttctataac actggcatac
     4021 ctatt
//
LOCUS       DATASH010000135         4591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4963604,
            whole genome shotgun sequence.
ACCESSION   DATASH010000135 DATASH010000000
VERSION     DATASH010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4591
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4963604"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..1984
                     /locus_tag="VHV99_04025"
     CDS             44..1984
                     /locus_tag="VHV99_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007737851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEX3634649.1"
                     /translation="MLDFFRNHKRLMMFMLILVIVPGLGFVGIQGFRGFFDESANVAS
                     VNGHKITRAEYDDAMRQQLDRARQMLGAQFDMKSFDTPERRSEMLDGLIEQRVLADET
                     QRLHLTASDDAVRRVLLNDPVISSLKNPDGSIDVDRYKQLLAMQGMTPDQYDERVRYS
                     IATQQLPASIQGSAFTSKTLAQHLTELAEQQREVQGIAFHPRDYAAKVQPTDAQLQAY
                     YDAHRNDFATPATATIQYLVMSPATLGAAAVQPSDADLKKYYDDNIAHYRTDGQVRAS
                     HILIAAPKDASAADKAKAKQKAEDLLAQIKAHPDQFAQIAQQNSQDPGSASKGGDLGY
                     FGRGMIAGGQAFDDAVFGLKKDEVSGIVQTDFGYHIVKVTDVKPAVTRPFDEVKDQIA
                     KDLKTQQASKAFTDDSEGFTSIVYEKAKSLQPAADKYKLQLQTATVTPQPDPKLPPDS
                     PLNNAKFLTAVFANDAVTARNNTQAIDVGGNTLIAARVTDYKAASVPALDAVKDAVRQ
                     KVIAVQSNETAHKEGIAKLAEFDKSKATTGFSSPLKVSRNDAQGVPPAALSAIYKADA
                     QKLPAYVGVDLGDGGYAIYRVNSVVAGAPIDPQRLAAAQQQIAQVDAQSEAEAYVEAL
                     RARSKVKFYGSLDSSNAQASGD"
     gene            complement(2119..2460)
                     /locus_tag="VHV99_04030"
     CDS             complement(2119..2460)
                     /locus_tag="VHV99_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634650.1"
                     /translation="MKYLPLIALTVAISACAAQPPAGVQSVGQSQQPPKAVATCIAQK
                     WADSSQQQVVSQDTLANDQAMDVYVPGQQPPNGAAAVVRPSYTGPGSWVGFRANGAAG
                     SDTTSSISACL"
     gene            complement(2578..3282)
                     /locus_tag="VHV99_04035"
     CDS             complement(2578..3282)
                     /locus_tag="VHV99_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018432015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEX3634651.1"
                     /translation="MVKLRLKVRAVATRAPALTTAFGSAVLAATLFSLPFPADAANAP
                     EPAKPVIVVLGDSISAEYGLPRDTGWVALMRQRLTDERMDYSVANASISGDTTSGGRA
                     RLPALLQRLKPSIVIVELGANDALRGVPLSTTEDNLRTIIEQTQQSHAKVVLVGMYVP
                     PNYGPDYTQKFHGMYGELSTQLKVPLVPFLLAGIADKPDMFQADQFHPTQQAQPVLLN
                     NVWPAIKPLLRTSSPH"
     gene            3281..3967
                     /locus_tag="VHV99_04040"
     CDS             3281..3967
                     /locus_tag="VHV99_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3634652.1"
                     /translation="MLNKTDPVIEVRGLCKKVKDATGELTILEDIDLAIDAGSSVAIV
                     GASGSGKSTLLGLLAGLDSASSGSVRLLGRELTDLNEDERAALRSGSVGFVFQSFQLM
                     PHLTALENVTLPLELQGGIGTREAAARARGLLEQVGLGKRTGHYPKLLSGGEQQRVAL
                     ARAFVTHPAILFADEPTGSLDAATGHAVIDLMFEMNRANGATLILVTHDIELARRCDT
                     TVTIDAGRLA"
     gene            complement(4160..>4591)
                     /locus_tag="VHV99_04045"
     CDS             complement(4160..>4591)
                     /locus_tag="VHV99_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012400422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEX3634653.1"
                     /translation="FIAVLTPEHPLVSHHPKLLANCFAQSEALMLGRTLEEAKKVAGA
                     DKPDLAPHLVFPGNRPTSTLLVEALTARSLGALIALYEHKVLVQASVWDINPFDQWGV
                     ELGKILGKVVEADLTSASVDEKKHDSSTSALIARARAALKR"
BASE COUNT          958 a         1364 c         1409 g          860 t
ORIGIN      
        1 ctgttgtaag atcgggcgtt ctgtttttgg cactccctca cgcatgctcg attttttccg
       61 caatcacaaa cgcctgatga tgttcatgct catcctcgtc attgtgccgg ggttgggttt
      121 tgtgggtatt caaggcttcc gcggcttctt tgacgaaagt gcaaacgtcg ccagcgtcaa
      181 cggtcacaag attactcgcg cagagtatga cgacgcgatg cgtcagcagc tcgaccgcgc
      241 ccgtcagatg ctcggcgcgc agttcgacat gaagtcgttc gacacgccgg agcgccgtag
      301 cgagatgctc gacggtctga tcgagcaacg tgtgctggct gatgagacgc agcgtctgca
      361 tctgaccgca tcggacgacg ccgtgcgccg tgtgctgctg aacgatccgg tgatctcttc
      421 gctgaagaat cctgacggct cgatcgacgt cgaccgttac aagcagttgc tcgcgatgca
      481 gggcatgacg cccgatcaat acgacgaacg tgtgcgttac agcatcgcca cgcagcagtt
      541 acctgcgagc attcaaggca gcgctttcac gtcgaagacc ctcgcgcagc atctgaccga
      601 actcgccgaa cagcagcgcg aagtgcaggg tatcgcgttc catccgcgtg actatgccgc
      661 gaaggtgcag cctacggatg cacaactgca ggcgtattac gacgcgcatc gaaatgactt
      721 cgcaacgccc gccacggcaa cgatccagta cctggtgatg tcgccggcaa cgttgggagc
      781 tgctgctgtc cagccgagcg acgccgacct gaaaaaatac tacgacgaca acatcgcgca
      841 ctaccgcacc gacggtcagg tgcgcgcgag ccacatcctg atcgccgcgc cgaaagatgc
      901 cagcgccgct gacaaggcca aggcgaagca gaaggcagaa gacctactcg cacagatcaa
      961 ggcgcacccg gaccagttcg cgcaaatcgc gcagcagaac tcgcaggatc cgggttcggc
     1021 gtcgaaaggt ggcgacctgg gttacttcgg tcgcggcatg atcgcaggcg gccaggcgtt
     1081 cgacgacgcc gtgttcggtc tcaagaaaga cgaagtgagc ggcatcgtgc agaccgactt
     1141 cggctatcac atcgtcaagg ttacggacgt gaagccggca gtcacgaggc cgttcgacga
     1201 agtgaaagat cagatcgcaa aagacctgaa gacgcagcag gcgagcaagg cattcaccga
     1261 cgactcggaa ggtttcacgt cgatcgtcta cgagaaggcg aagagcctgc agcctgccgc
     1321 cgacaagtac aagctgcaat tgcaaacggc tacggtgacg ccgcagccgg atccgaaact
     1381 gccgcctgac agcccgctga acaacgcaaa gttcctgact gctgtgtttg caaacgacgc
     1441 ggtcaccgcg cgtaacaata cgcaagcgat cgacgtcggc ggcaacacgc tgatcgcggc
     1501 acgcgtgacc gattacaagg ctgcgtccgt gccggcgctc gacgccgtca aggacgcagt
     1561 gcgtcagaag gtgatcgcgg tacagtcgaa cgagacagct cacaaggagg gcattgcgaa
     1621 gctggcggag ttcgacaagt cgaaggccac aacgggcttc tcatcgccgc tgaaggtttc
     1681 gcgcaatgat gcgcaaggcg tgccgccggc tgcgttgagt gcaatctaca aagcggacgc
     1741 gcaaaaattg cctgcgtacg tcggtgtgga tctcggcgac ggcggctatg cgatttaccg
     1801 cgtgaattcg gtcgttgcgg gcgctccgat cgatccgcag cgtttggctg ccgcccagca
     1861 gcagattgcg caagttgacg cgcagtccga agcagaggct tatgttgaag cgctgcgagc
     1921 ccgctcgaag gtgaagttct acggttcact cgacagcagc aacgcacagg cgagcggcga
     1981 ctaagcgcag cgcgcaagcg ggcaaagcga tacagataca aaaagccccg caatcgcggg
     2041 gctttttgtg tttaacgagt gcaagctcgt caagcggttt acccggaagc tctcggctcc
     2101 cttcataccg tcagtggctt agaggcaagc gctgatcgag ctggtggtgt cgctgcccgc
     2161 agcgccattc gcgcggaagc cgacccacga acccgggccg gtgtacgacg gacgtaccac
     2221 ggcggctgcg ccgttaggcg gttgctggcc cggcacatac acatccatgg cctggtcgtt
     2281 agccaaggtg tcttgcgaca cgacttgctg ttgcgagcta tcggcccact tttgcgcgat
     2341 gcaggtagcg acggcctttg gcggttgctg gctttgaccc acggattgga caccagcagg
     2401 cggttgagcg gcacatgcgg aaattgccac ggtcaaagcg attaacggta aatatttcac
     2461 gttatctcct cagaagtcgc tcagaatcga gccgggttac acgggattac gaaagtgtca
     2521 gattgacctt gccgatccgt gttcccaatt accggaaaca agttgttagc aagcttttca
     2581 gtgcggcgaa cttgtgcgaa ggagtggctt gatagccggc cacacgttgt tgagtagcac
     2641 cggctgtgcc tgctgcgtgg gatggaactg atcggcctga aacatatcgg gtttgtcagc
     2701 gattccagcg aggaggaagg gcaccagcgg aaccttcaat tgcgtcgaca gttcaccgta
     2761 catgccatgg aacttttgcg tgtagtcggg gccgtaatta ggcggcacgt acatgccaac
     2821 cagtacaacc ttcgcgtggc tctgctgagt ctgctcgatg atcgtacgca ggttgtcttc
     2881 ggtggtcgaa agcggcacgc cgcgcaacgc atcgttggcg ccgagctcca cgatcacgat
     2941 gctcggcttg agacgttgca atagcgccgg caagcgcgcc cgtccgccgc ttgtcgtgtc
     3001 gccgctgatg ctcgcattgg cgacgctata atccattcgc tcgtcggtga ggcgctggcg
     3061 catcaaggca acccagcccg tgtcgcgggg caagccatac tcggccgaga tactgtcgcc
     3121 gagtacgacg atcaccggct tggccggttc gggcgcgttg gccgcatcgg ctgggaaggg
     3181 cagcgaaaac aaggttgcgg ccagcacggc cgagccgaat gccgtagtca gcgccggcgc
     3241 gcgagtcgct acggcgcgca ctttcaacct aagcttcacc atgctaaaca aaactgatcc
     3301 agtcattgaa gtgcggggtt tgtgcaagaa ggttaaggat gcaacaggcg aactgacgat
     3361 tctcgaggac atcgatcttg ctatcgatgc cggcagcagt gtggcgatcg tcggtgcatc
     3421 agggtcgggc aagtctacgc tgcttggctt gctcgcagga ttggacagcg cgagctcggg
     3481 ctcggttcgg ctgctcggcc gcgaactcac cgatctgaac gaagacgagc gcgccgcgtt
     3541 gcgcagcggc tcggtcggtt tcgtgttcca gtcgtttcaa ctcatgccgc atttgacggc
     3601 cctcgaaaac gtcaccttgc cgctcgaact gcaaggcggc atcggcacgc gtgaggctgc
     3661 ggcgcgcgcg cgcggtttgc tggagcaggt gggtcttggc aagcgcactg ggcattatcc
     3721 gaagctgctg tcgggtggtg agcagcaacg agtcgccctc gctcgcgcgt ttgttacgca
     3781 tccggcaatc ctgttcgccg acgagccgac cggcagcctc gatgccgcga ccggtcatgc
     3841 agtgatcgat ttaatgttcg agatgaaccg cgcgaacggc gccacgttga ttctcgtcac
     3901 gcacgatatt gagctcgccc gccgctgcga cacgacggtg acgatcgacg cgggacggtt
     3961 ggcctgagaa cggactgcat gccgttcacg ccatcaatag tcaccgggcc agcgtaccgg
     4021 ccttcttcgt gtgaggcgac gcgtcgcgcg gcaagattcg aatcgcctga aaaacaaaac
     4081 cccacgctcc gcaaaacgtg gggtcggtca gcagtctgaa gccggccgca aaggcccgct
     4141 ttttatctca gcgaacgtct caccgcttca acgcagcacg cgcccgcgca atcaacgccg
     4201 acgtcgacga gtcatgcttc ttctcatcaa cgcttgccga ggtcagatcc gcctcgacca
     4261 ctttgccgag gatcttgcct agctcgacgc cccactgatc gaacggattg atgtcccaca
     4321 cggatgcctg caccagcacc ttatgctcat acagcgcgat caatgcgcca agcgaacgcg
     4381 cggtgagcgc ttcgacgagc agtgtggagg tcggccggtt gcccggaaac accaggtgcg
     4441 gcgcgaggtc aggcttgtcc gcgccggcaa ccttcttcgc ttcttcgagc gtgcggccga
     4501 gcatcaacgc ttcactctgc gcaaaacagt tcgccagcag cttcggatga tgactgacga
     4561 gcggatgctc cggcgtcagc acggcgatga a
//
LOCUS       DATASH010000136         4292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2787304,
            whole genome shotgun sequence.
ACCESSION   DATASH010000136 DATASH010000000
VERSION     DATASH010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2787304"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(276..782)
                     /locus_tag="VHV99_04050"
     CDS             complement(276..782)
                     /locus_tag="VHV99_04050"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3634654.1"
                     /translation="MTESQQTAATRLAQLEWRWKAFEDLTTAEVYAMLAARSAVFVVE
                     QNCVYGDVDGLDSDAWHLFAFGPGGTGNNQRPPLAGYLRVLLPDADDAEIRIGRVLTT
                     AEFRGIGLGNAMLERSLAHIRAQWPGTPIRLHAQAHLQGFYGAFGFTPVSEIHEEDGI
                     PHVWMRSV"
     gene            complement(993..1973)
                     /locus_tag="VHV99_04055"
     CDS             complement(993..1973)
                     /locus_tag="VHV99_04055"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEX3634655.1"
                     /translation="MQVPLTPSNESARLDTLRALNILDTLPEERFDRLTRLAKRLFDV
                     PIALVSLVDEDRQWFKSCIGLAASETSRDVSFCAHAILDDDILMVPDTLADARFHDNP
                     LVTDDPNIRFYAGCPLTVPNGSKLGTLCLIDTKPRGLDEEERELLRDLAHMAEQELVA
                     VQLATLDDLTLLSNRRGFEALSQHALNVCKRTDKPASLLFFDLNDFKLINDIHGHAEG
                     DLALKAFAEVLQNALRESDVIGRLGGDEFVALLTNSGSGETLEVRQRLTALLDARNRK
                     AQRGYQIRYSVGQIQYDPARHASIADLLAAADAAMYSHKQASKARAVHGS"
     gene            complement(2176..2934)
                     /locus_tag="VHV99_04060"
     CDS             complement(2176..2934)
                     /locus_tag="VHV99_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HEX3634656.1"
                     /translation="MYLQLTGTNIRLLGSMHLFPATSRRTPRWIGHAYDWAESLVFES
                     DPPTILPYLKTHPQGGAAQLQPLLSADAWNQLQAAWPAEGPLAPLVDLRPWAALIVAP
                     TLFQQVVEGVEPRMLRSALTQAKPYQYLETAQDVAASLEAIPLDAIGTALGMLMTDLA
                     EPQRTLERMHAAWLHGDLPTVQQIAIESPMFNLPGIREAILDARNRAWAARVTALLGR
                     RERTLVVVGALHLCGPGNLIECLAQAVEPVSVGE"
     gene            complement(2831..3367)
                     /locus_tag="VHV99_04065"
     CDS             complement(2831..3367)
                     /locus_tag="VHV99_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634657.1"
                     /translation="MRRFLHLRLIYALCLCRATGTHLQVVLVHGLWWDYGGAAPLTRI
                     HWTSLLCIDPLTALLLPWRPEAGPILCVAVIVSDVVHNSWFALHHPIRMDLYLSQVVF
                     LLFVACTVRTSGKGPRVAPPHCASSIELPDCLFVQLPVYARLHVSTTYRHQHSSARLN
                     ASVSCDEPQNAAMDRARV"
     gene            complement(3415..3488)
                     /locus_tag="VHV99_04070"
     tRNA            complement(3415..3488)
                     /locus_tag="VHV99_04070"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3454..3456),aa:Cys,seq:gca)
     gene            complement(3601..3676)
                     /locus_tag="VHV99_04075"
     tRNA            complement(3601..3676)
                     /locus_tag="VHV99_04075"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3641..3643),aa:Gly,seq:gcc)
     gene            complement(3740..3815)
                     /locus_tag="VHV99_04080"
     tRNA            complement(3740..3815)
                     /locus_tag="VHV99_04080"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3780..3782),aa:Gly,seq:gcc)
     gene            complement(4021..>4292)
                     /locus_tag="VHV99_04085"
     CDS             complement(4021..>4292)
                     /locus_tag="VHV99_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX3634658.1"
                     /translation="SALREWMAQIGASKSVAVNSLGKFKTVCQMVAIPMLLFYGPLPL
                     GGGVVVDTRVWGLWLIYLAAFLTIWSMLYYMKLAWPQIRERGGVA"
BASE COUNT          864 a         1331 c         1329 g          768 t
ORIGIN      
        1 ggcgcgttgc tggcgttgcg tcactggatg cggctgtcgg cacgcggacg tctgcgcggc
       61 gagcggcgcg ccacggcgac ccgccacgac ctggcgcaag ccgcctcagt gcccgcgggc
      121 ggccagcact gatcccttga agcacttgac gacggaatca ggggcgggcc gaagcgcagg
      181 atagggtcct gcaatgagga tgtccaaggg gctttgatgt cgcagccttt gaagccgctc
      241 gatgcgcgca cgtcgtgcac ggtttgcagt ctggcttaga ccgaacgcat ccacacgtgc
      301 gggatgccgt cctcttcgtg aatctccgac accggcgtaa agccgaacgc gccgtagaag
      361 ccctgcagat gcgcctgcgc atgcaaacgg atcggcgtac caggccactg tgcgcgaata
      421 tgcgcgagcg agcgttcgag catcgcgttg ccgagaccaa tgccgcggaa ttccgcggtg
      481 gtcaacacgc ggccgatgcg gatctcggcg tcgtcagcat cgggcagcag tacgcgcaga
      541 tagcccgcga gcggcggacg ctggttgttg cctgttccgc cgggaccaaa ggcaaacaga
      601 tgccaggcat ctgagtcgag cccgtcgaca tcgccataca cgcaattctg ctcgacgacg
      661 aacaccgcgc tgcgagcggc cagcatcgca tagacttcgg cggtcgtaag gtcttcgaac
      721 gctttccagc gccattcgag ttgcgccagt cgcgtggcgg cggtttgctg cgattctgtc
      781 atgggaaatc tttaaaggaa gcgccccgta acgggcggtg gcaatgcgga attatgccgt
      841 gcgtgggggg cttgggctat gcgcgccggg ccggcgccga ttctcgtgag gtcgacgcga
      901 aagtacggcg gcgagtgctg tgtatcgtgt cgtgaccgga atccgcggca cgccacgtgc
      961 gcgtaccgga aacgctcgcg ttcatgccac gctcaactgc cgtgcaccgc ccgtgccttc
     1021 gacgcctgct tgtggctata catcgccgca tcggcagccg cgagcaagtc ggcgatcgat
     1081 gcatgccgcg cgggatcgta ctgaatctgg ccgacgctat agcggatctg gtagccacgc
     1141 tgtgctttgc gattgcgcgc atccagcaat gccgtgagac gctgcctgac ttccagcgtc
     1201 tcgccgctgc ccgagttggt cagcaaagcg acgaactcgt cgccacccag ccgcccgatc
     1261 acatcgcttt cgcgcagcgc gttttgcaac acctcggcga aggccttcaa cgcgaggtcg
     1321 ccttccgcat ggccgtggat gtcgttgatc agcttgaaat cgttcagatc gaaaaacagc
     1381 agcgacgccg gtttatccgt ccgcttgcag acgttcaatg catgttgcga gagcgcttcg
     1441 aaaccgcgac ggttcgacag caacgtcagg tcgtcaagcg tggccagttg cacggccacc
     1501 agttcctgct cagccatatg cgccagatcg cgcagcaatt cgcgctcttc ctcgtcgagg
     1561 ccgcgcggct ttgtgtcgat cagacacagc gtgcccagct tgctgccatt cggaaccgtg
     1621 agcggacagc cggcgtagaa acggatgttc ggatcgtccg tgacgagcgg attgtcgtgg
     1681 aaacgcgcgt cggccagtgt atccggcacc atcagaatgt cgtcgtcgag aatcgcgtgc
     1741 gcgcagaacg acacgtcgcg cgacgtctcg gacgctgcca gcccgataca cgatttaaac
     1801 cactgcctgt cttcatcgac gaggctcacg agcgcgatcg gcacgtcgaa tagccgtttg
     1861 gcgaggcgag tcaggcggtc gaagcgctct tcaggcaagg tatcgagaat attcagcgca
     1921 cgcaatgtgt cgagacgggc gctttcattg ctgggcgtca gtgggacttg catggggtat
     1981 tccgtagagc cataaggggt gaccgggttc gtgagcacgt agtgtatagc ggcatttttc
     2041 acgtccgatt gagcccgtcc cgcagatttc cgccgggttg cgtcggagcc gtgttgtcgc
     2101 cgtgccccgt caggcgccgc aatcaggaca tttcacggtc cattgcggct cgcgggctgc
     2161 cccggcggca agccgttact cgccgacgga aaccggctcg acggcttgag cgagacactc
     2221 gatcaaatta ccgggcccgc acaaatgcag cgcgcccact accaccaggg tccgctcccg
     2281 gcgcccgagc agcgccgtga cgcgcgccgc ccacgcgcga ttgcgggcgt cgagaatagc
     2341 ctcgcggata ccgggcagat tgaacatcgg cgactcgatg gcaatctgct gcacggtcgg
     2401 caaatcgcca tgcagccagg cggcatgcat tcgctcgagt gtgcgttgcg gttcagcgag
     2461 atcggtcatc agcatgccga gcgccgtgcc gatagcgtcg agcggaatcg cttcgagcga
     2521 cgccgccacg tcctgcgcgg tctcgagata ctggtacggt ttggcttgtg tgagcgccga
     2581 gcgcagcatg cgcggctcga cgccttcgac gacttgctgg aacagcgtcg gcgcgacgat
     2641 cagcgcggcc cacggacgca gatcgaccag cggcgccaac gggccttccg cgggccacgc
     2701 ggcttgtagc tgattccagg catcggcgga tagcaagggc tgcagttgcg ctgcgccgcc
     2761 ctgcggatga gtcttcagat acggcaggat ggtcggtgga tcggattcga agaccagcga
     2821 ttcggcccag tcatacgcgt gcccgatcca tcgcggcgtt ctgcggctcg tcgcaggaaa
     2881 cagatgcatt gagccgagca gacgaatgtt ggtgccggta agttgtagat acatgcagcc
     2941 tcgcgtagac gggcaattgc acaaagaggc aatcgggcaa ttcaatcgac gacgcgcagt
     3001 gcggcggcgc cactcgcggc cccttgccag acgtccgcac cgtgcacgcg acgaacagca
     3061 ggaagacgac ctgcgaaaga tacagatcca tgcgaatcgg atgatgcagt gcgaaccatg
     3121 aattgtgcac tacgtccgac acgatgacgg cgacgcagag gatcggcccg gcttctggtc
     3181 gccacggcag tagcagagcg gtcagcggat cgatgcagag caatgaggtc cagtggatcc
     3241 gcgtcagcgg cgccgcgccg ccgtagtccc accagagtcc atggaccaag accacttgca
     3301 gatgcgtccc ggtagcgcgg caaaggcaga gtgcatagat caggcggagg tggagaaaac
     3361 ggcgcataaa aagaaaagcc ccgacaagtc ggggcttttc tttacatcaa ctactggagg
     3421 cgcgagccgg agtcgaaccg gcctaaacgg ctttgcaggc cgctgcataa ccgctttgct
     3481 atcgcgccgc aagcggactg gaacctgagt gcaggctcgc agttttgttc ggcggacaat
     3541 cgctcaacca gaacgatcgg cccaccaaac aaaaagggaa gcgctgcttc cccttattta
     3601 tggagcggga gacgaggctc gaactcgcga cctcaacctt ggcaaggttg cgctctacca
     3661 actgagctac tcccgcattg cactgcttca caacgcgctg cacttttact tcgccaaaca
     3721 acgcggtgct tcaagaaact ggagcgggag acgaggctcg aactcgcgac ctcaaccttg
     3781 gcaaggttgc gctctaccaa ctgagctact cccgcattgt gctgctaact actgccttat
     3841 ttactgcaac tactctgcta cttcgtccgt cgcctcaagc tgcgtgcatc ggagaaacga
     3901 gattatggag aaacgactga aacgtgtcaa ccccttttgc gaatctcttt tactgaacag
     3961 atttcactca tgcgtcagcg aagttcggac acactccagg cgctctcgac cactgcaaag
     4021 tcaggccacg ccaccccgct cgcgaatctg cggccacgcg agcttcatgt agtagagcat
     4081 cgaccagatc gtcaggaacg cggccagata gatgagccac aggccccaca cgcgtgtgtc
     4141 gaccaccacg ccgccgccca acggcagcgg gccgtaaaac agcagcatgg gaatcgccac
     4201 catctggcaa acggtcttga acttgccgag cgaattcacg gcgacgctct tcgacgcgcc
     4261 gatctgcgcc atccactcac gcagcgccga ga
//
LOCUS       DATASH010000137         8502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1008195,
            whole genome shotgun sequence.
ACCESSION   DATASH010000137 DATASH010000000
VERSION     DATASH010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 8502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8502
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1008195"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /gene="xseA"
                     /locus_tag="VHV99_04090"
     CDS             <1..983
                     /gene="xseA"
                     /locus_tag="VHV99_04090"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEX3634659.1"
                     /translation="GLFAAERKRPLPAHPRAIGIVTSLQAAALRDVLTPLSRRAPHIP
                     VIVYPAPVQGVGVSAKLAAMVDAANARREVDVLIVCRGGGSIEDLWAFNEQVLARAIA
                     ESAIPVVSGVGHETDFTIADFAADVRAPTPTGAAELVSPQRVLLLRELDHRHATLARG
                     FGRMMERRAQQLDWLARRLVSPAERLARQRTHLQQLSVRLASAGARPARDARARFSLL
                     QMRWQRWRPDLAAHQAKLGNLAQRLDAALLRQHERQSARIDTLSARLEVLSPQRTLER
                     GYAAVLDAQSGRAVRAPSSLKPGRRLTVHLAEGAADIALADVQPRLTDGF"
     gene            1204..1782
                     /gene="sodB"
                     /locus_tag="VHV99_04095"
     CDS             1204..1782
                     /gene="sodB"
                     /locus_tag="VHV99_04095"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006024786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HEX3634660.1"
                     /translation="MEHTLPPLPFAKSALAPNMSEETLEYHYGKHHQTYVTNLNNLIK
                     GTEFENLSLEEIVKKASGGIFNNAAQVWNHTFFWNSLSPQGGGAPTGALADAINAKWG
                     SFDKFKEEFAKTAIGTFGSGWAWLVKKADGSLDLVSTSNAATPLTTDAKALLTIDVWE
                     HAYYIDYRNARPKFVEAYWNIANWEFASKNFA"
     gene            complement(2163..2441)
                     /locus_tag="VHV99_04100"
     CDS             complement(2163..2441)
                     /locus_tag="VHV99_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634661.1"
                     /translation="MNRLVFVAATACAACISPVFAQSNATVQSSVLEPSTTTFPSASI
                     AQNFQYINHPTPPAPASSVSPSGGRRGGGRHRQMSADSDTTGTASTQP"
     gene            2789..3364
                     /locus_tag="VHV99_04105"
     CDS             2789..3364
                     /locus_tag="VHV99_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HEX3634662.1"
                     /translation="MISSTKSISIAIPAAVVALALGGCAVMPPGGPSVVALPRSGEPL
                     GQFQQDDYACRDYANHSTDPNAAAQAATTNSVNSAALGTLGGAAVGALLGAAAGNAGA
                     GAAIGAGSGLLIGGANGANGAQYSAAGLQARYDAAYAQCMTSKGNTISQPQAPAYYAP
                     QPAYYAPQPYYYAPPPRYVAPPPVMYAPYPY"
     gene            3655..5427
                     /locus_tag="VHV99_04110"
     CDS             3655..5427
                     /locus_tag="VHV99_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrrolo-quinoline quinone"
                     /protein_id="HEX3634663.1"
                     /translation="MQRPQTGTFNPRVWSRVCGWLGVAFVAACFLSCGGGSGGTSGVA
                     SGSAGGSSGSGSTGGSTSGGTGGTGSSGGSSTGSSGSGSTSTGTTAFATDVLMHHNDL
                     ARTGQMLAETTLTTANVKSASFGKVAFLAADGKVDAQPLFVTSLPIGGTPHDVVYVAT
                     EHDSVYAYDATSFAQLWKVSLVGNGETPSDDRGCQDITPEIGITSTPVIDRNRGTNGV
                     LYAVAMTRDSSGAYHHRLHALDLTTGAEVLGGPTEIAATFPGNGAEGVNGVLTFNPSL
                     HTVRAALTLVGGNIYMGWTAHCMSGAYTGWLMAYSAATLQQTSVFDVVPNGSQGSIWM
                     AGSGMASDGTSIYVVDGNGTFGTTLNAQGFPVDGDFGNSLMKLSLGNLQVTDYFAMDN
                     VVAEANADNDFGSGGVMLLPDQTTTAGVVKHLAVAAGKDNLIYVVDRDSMGKFSPTAN
                     NIWQVLTGTLAGGIWGSPAYYNGVVYYGGMGDSLKALPVSGAFLATTASSRSAITFVY
                     PGATPAVSANGTSNGIVWAAENGATGALHAYDAGNLGNELYNSNQAGARDQWGAGNKF
                     ITPMIARGKVYVGATNGVAVFGLL"
     gene            complement(5730..7166)
                     /locus_tag="VHV99_04115"
     CDS             complement(5730..7166)
                     /locus_tag="VHV99_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX3634664.1"
                     /translation="MASSSETVSMALFCDFENVALGVRDAKYDKFDIKLVLERLLLKG
                     SIVVKKAYCDWDRYKSFKGAMHEANFELIEIPHVRQSGKNSADIRLVVDALDLCYTKS
                     HVNTFVIISGDSDFSPLVSKLRENAKQVIGVGVQQSTSDLLIANCDEFFFYDDLVRES
                     QRTVAKRESSRPQQATQQTAKRAPDEEKSRNKEDLEKRRTKAVEIAVQTFDALASERG
                     DSGKIWASVLKNAIKRRKPDFNETYYGFRAFGNLLEEAQARGLLEFGRDEKSGAYVYR
                     GTAASAGAENVSEPAESSESAEQMYEAQVAETVVVESGGKHESRRRGRGNRKTGRGGQ
                     QEASHEERAALGRGDHTQVHTEHVQPQSPSTSEPSFEQPEAAWVEPETFATAPPFAVS
                     NEEAQEESVAQPKRGKERAPRKTAAKKAKKATPRTIDDIPEIAAPAEAPEPSVSTEEA
                     PVAAKKAARKTAPRSRRPRKTAATSDAE"
     gene            complement(7517..8164)
                     /gene="clpP"
                     /locus_tag="VHV99_04120"
     CDS             complement(7517..8164)
                     /gene="clpP"
                     /locus_tag="VHV99_04120"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEX3634665.1"
                     /translation="MHSSYPCVTGLGLVPTVIEQSGRGERAYDIYSRLLRERIVFLVG
                     PVNEQSASLIVAQLLFLESENPDKDISFYINSPGGSVYDGLAIYDTMQFIKPEVSTLC
                     TGFAASMGTFLLTAGQRGKRYALPNARIMIHQPSGGGQGTAADVEIQAKEVLYLRERL
                     NAMMAERTGRSIEEIVRDTDRDNFMSAHGAKAYGLVDEVLETRAALRDPARHRDM"
BASE COUNT         1538 a         2593 c         2733 g         1638 t
ORIGIN      
        1 aaggtctctt tgccgccgaa cgcaaacggc cgttgccggc ccatccgcgc gcgatcggca
       61 tcgtgacatc gctgcaggcc gcggcattgc gtgatgtcct gacccccttg tcgcgtcgcg
      121 cgccgcatat tccggtgatc gtctatcccg caccggtgca gggcgtgggc gtcagcgcga
      181 agctggccgc gatggtcgat gcagccaacg cccggcgcga agtcgatgtg ctgatcgtgt
      241 gccgcggcgg cggctcgatt gaagacttgt gggcgttcaa cgaacaagtg cttgcgcgcg
      301 cgatcgctga aagcgcgata ccggtggtaa gcggcgtggg tcacgaaacc gatttcacga
      361 tcgccgactt tgccgccgac gtgcgcgcac cgacgcccac cggcgccgcc gaactcgtca
      421 gtccgcagcg cgtgctgttg ctgcgcgaac tcgatcaccg gcatgcgacg ctcgcccgcg
      481 gcttcggtcg catgatggag cggcgcgccc aacagctcga ctggctcgcg cgccggctcg
      541 tgtcgcccgc ggagcggctc gcgcggcagc gcacgcacct gcagcaattg agcgtgcggc
      601 tggcatcggc gggcgcgcgt cccgcacgtg acgcccgagc gcggttttct ctgttgcaga
      661 tgcgctggca gcgctggcgc cctgatctcg ccgcgcatca ggcgaagctc ggcaatctcg
      721 cgcagcgtct ggacgctgct ttgttacgcc agcatgaacg tcagtcagcg cggatcgaca
      781 cgctgtccgc gcggctcgag gtgttgagtc cgcagcgcac gctcgagcgt ggctatgctg
      841 ctgtgctaga cgcgcagagc ggccgcgcgg tgcgcgcgcc gtcgtcgctg aaacccgggc
      901 ggcgcctcac ggtgcatctt gccgaaggcg cagcggacat tgcgctcgcc gatgtgcaac
      961 cgcgcttgac cgacggtttc tgacacttcg ccgacagtat gccaaggcgt acttttgacc
     1021 tctgcagggg ccgggcacga cccctgtgaa cgcattaatt acgctatata tccggcctgt
     1081 tcgccgatgc cggagataaa gtccgttggt ttgcggggtt attcaacctc gcctacaatc
     1141 gagtgctcta aagcgttatc cgcagactcc ccccacaaca gatacagaca caaggaaagc
     1201 accatggaac atacgctccc gccgctgccg ttcgcaaaaa gcgcactcgc tccgaacatg
     1261 tcggaagaga cgctcgagta tcactacggc aagcaccacc agacctatgt gaccaacctg
     1321 aacaatctga tcaagggcac cgagttcgaa aatctgtcgc tcgaagagat cgtcaagaag
     1381 gcatcgggcg gtatcttcaa caatgcagcg caagtgtgga accacacgtt cttctggaac
     1441 agcctgtcgc cgcaaggtgg cggtgcaccg accggcgcat tggccgacgc gatcaacgcc
     1501 aagtggggtt cgttcgacaa gttcaaggaa gaattcgcca agaccgcaat cggcacgttc
     1561 ggctcgggct gggcatggct ggtgaagaag gctgacggtt cgctcgacct ggtgtcgacg
     1621 agcaacgctg ccacgccgct gaccaccgat gcgaaggcac tgctgacgat cgacgtgtgg
     1681 gaacacgcgt actacatcga ctaccgcaat gcgcgtccga agttcgttga agcatactgg
     1741 aacatcgcca actgggaatt cgcgtcgaag aacttcgcgt gagattgctg gttgccggct
     1801 gcttgtctta gctgaaaact tagcgtgaca acatagttcg gaattccaac taaatcggcg
     1861 gtccgaacgg atcgccgcac tgaagtctgg aatgggtagt aaaacggaaa gccctccttc
     1921 gcgagggctt tccgtttttt agcgcctgga gagcgggtac agcctgccgc cggttgacgc
     1981 caaacggagc ccgtcgttcc cgacgtgacg aatcgattga cgaccgtgat ctacgccggc
     2041 acgctggtca atggcgcccg cctgccggac cgaaggcgag tgccagcttt gtcacagttg
     2101 gaaactgcgg cgacgtccgc ggaccattgc accgcattga ttcagacctt gcgcaggttc
     2161 gttcaaggct gtgtcgacgc ggtcccagtc gtatcggaat cggccgacat ctgccggtgg
     2221 cgaccgccgc cgcgtctgcc gccgctgggc gaaaccgaac tcgccggcgc gggcggcgtc
     2281 gggtgattga tgtactggaa attctgcgcg atcgacgcac tgggaaatgt cgtggtggac
     2341 ggttcgagca cactgctctg gactgtcgca ttcgactgcg cgaaaacggg cgatatgcag
     2401 gcggcgcaag ccgtcgctgc tacgaaaaca agccggttca tcgagtcatc ctttgtttta
     2461 ttgaccgtgt ccattgagcg tgcgagggca atgtgcccgt tcgccccagt cggactgcct
     2521 tggcatgcca cgtaccgtgc cgttcggcga gcggattgcg caatgttggc gcgagccagt
     2581 ttaggccgtg cactgagaaa acagattgcg gcttcactta caagcgttac tcgatatgac
     2641 gtgattgagt acagcttgca gggcgcgatg ccgtccgcgc ggacccgggc gggaatgtaa
     2701 gcgaaaaact ttctgtaacg atcatgtaac cagggacggt gccgtttggc ctaacgtttg
     2761 aattccactg tcctagaagc gcaccaacat gatctccagc accaaatcga taagcatcgc
     2821 cattcccgcc gcggtcgttg cgctcgcgct cggcggttgc gcggtcatgc cgccgggcgg
     2881 tccgagtgtg gttgccctgc cgcgcagcgg cgagccgctc gggcaattcc agcaggacga
     2941 ctatgcatgc cgcgactacg cgaaccattc gaccgacccg aacgcggccg ctcaggcggc
     3001 aaccaccaac agcgtgaata gtgcggcgct tggcacgctc ggcggcgcgg ccgtcggcgc
     3061 actgctcggc gcggcggcgg gcaatgccgg cgcgggcgcg gcgatcggcg cgggcagcgg
     3121 attgttgatc ggcggcgcga acggggcaaa tggcgcgcag tattccgccg ccggcctgca
     3181 agcgcgctac gacgccgcgt acgctcagtg tatgacctcg aagggcaaca caatttcgca
     3241 gccgcaggcg ccggcctact atgcaccgca accggcctat tacgcaccgc agccgtatta
     3301 ctacgcgccg ccgccgaggt atgtcgcccc gcctccggtg atgtacgcgc cttacccgta
     3361 ctagaggtcg atcggcagcc gcgcttcact gaaacgagcc gtttgcgcca ggtaccaggc
     3421 aagcttgttg cttccaggct ttcgtccggg ctggcggaaa gacgcgaaac gatggcggca
     3481 agcggcccgg caaagggcgt aggttgggga caagggctag ggcgccgggt atctggcgct
     3541 cctgcgcagc ccgcaactcc actccatccc tgccggccaa cgctgtttct gacttcgccg
     3601 ttcgacgtag ccctgcgtac ggacctggcc gggcatcgtg caaacgaggc gactatgcag
     3661 cgaccacaaa ccgggacgtt caatccgaga gtgtggtccc gcgtgtgcgg gtggctgggc
     3721 gttgcattcg tcgctgcctg cttcctttct tgcggcgggg gctcgggtgg tacatcaggt
     3781 gtcgcctcgg gttcggcagg cggcagcagc ggcagcggca gtacaggtgg ctcgaccagc
     3841 ggcggcacgg gcgggactgg ctcaagcggc ggctcaagca ccggctcgag cgggtcgggc
     3901 agcacatcca cgggaacgac ggccttcgcc accgacgtgc tgatgcatca caacgatctc
     3961 gcccgcaccg ggcagatgct tgccgagacg acactgacaa ccgcgaacgt caagtcagcc
     4021 agcttcggca aggttgcgtt tctcgctgcg gacgggaagg tcgacgcgca accgctgttc
     4081 gtgaccagtc tgccgattgg cgggacgccg catgacgtgg tctacgtcgc gaccgaacac
     4141 gacagtgtgt acgcctatga cgccaccagc ttcgcccagt tgtggaaggt ctccctggtc
     4201 ggcaatggcg agacacctag tgacgaccgc ggttgccagg acatcacgcc ggaaatcggc
     4261 attacgtcga cgccggtgat cgatcgcaat cgcggcacca atggcgtgct gtatgccgtg
     4321 gcgatgacca gggacagcag cggtgcatac caccatcgtc tgcacgcgct cgatctgacg
     4381 acgggggccg aggtgctcgg cggtccgacg gagatcgcgg cgactttccc cggcaacggt
     4441 gcggagggcg tcaacggcgt cctcacgttc aatccgtcgc tccacaccgt acgcgcggcc
     4501 ctcacgctgg tcggcggcaa catctatatg ggctggaccg cgcactgcat gtcgggcgcc
     4561 tacaccggct ggttgatggc ttatagcgct gctacgctcc agcagaccag cgtcttcgac
     4621 gtcgtgccga acggcagcca gggttcgatc tggatggccg gctcggggat ggcgtcggac
     4681 ggcacgtcga tttacgtcgt ggacggcaac ggcacattcg gcaccacgct gaacgcgcag
     4741 ggctttccgg tcgacggcga cttcggcaac tcgctgatga agctgtcgtt aggaaacttg
     4801 caggtcaccg attacttcgc gatggataac gtcgtggccg aggcaaacgc ggacaacgat
     4861 ttcggctcgg gtggcgtgat gctgttgccg gatcagacga ccaccgctgg cgttgtcaag
     4921 cacctcgcgg tggcggcggg caaggacaac ctgatctatg tggtcgatcg cgactcgatg
     4981 ggtaaattca gtccaacagc gaacaatatc tggcaggtgt tgaccggcac gctcgcgggc
     5041 ggtatctggg gttcgccggc ttactacaac ggggtggtgt actacggcgg catgggcgac
     5101 agcctgaagg cgctgccggt ctcgggtgcg ttcctcgcga ccaccgcttc atcaaggagt
     5161 gcaatcacct ttgtctatcc cggcgcgaca ccggccgtgt cggccaacgg cacgagcaac
     5221 ggtattgtct gggctgcgga aaacggcgcg acgggcgcgt tgcatgcgta cgatgccggc
     5281 aatctcggga atgagttgta caacagcaat caggccggtg cgcgcgatca atggggcgcg
     5341 ggcaataaat tcatcacgcc gatgatcgcg cgagggaaag tgtatgtggg ggcgactaat
     5401 ggggttgcgg tgtttgggtt gctttgagcg ttgcgggttt gggcgcggtt ggcgttgcga
     5461 ttgcagttgc agttgcagtt tgcggttgca gggccgctag ctggtgaata tgtgttctgt
     5521 tggcgccgtt gcgtttcaaa ccgattgctg cgcttccgag cggaaatggg aaagcccctg
     5581 ttcatcacaa gcaggggctt tttgttgccg ctgcgtttat acggcggcgc tcaggttgct
     5641 acgggcgaca gcgaggtgct acgcgggcaa tgccgaagtg ccacgggcaa cgccgaagca
     5701 gttcgcgcac atcgcgcgaa gcgagacgct tactccgcgt cgctcgttgc cgctgtctta
     5761 cgcggacgac ggctgcgcgg cgcggttttg cgcgcggcct tttttgccgc cacgggtgct
     5821 tcttccgtgc tgacggacgg ctccggtgct tcggcaggcg ccgcgatctc cggtatgtca
     5881 tcgatcgttc gcggagtggc ctttttcgcc ttcttcgccg ccgtcttgcg tggcgcgcgt
     5941 tccttgccgc gtttgggttg agcgacgctc tcttcctgtg cctcttcgtt gcttaccgcg
     6001 aacggtggtg cagttgcaaa ggtctccggt tccacccaag ccgcttctgg ctgttcaaag
     6061 ctgggctcgc tggttgatgg cgattgcggc tgcacgtgtt ccgtgtgaac ttgcgtgtga
     6121 tcgccgcgcc cgagcgccgc gcgctcttcg tgagaggctt cctgctgccc accacggccg
     6181 gtcttgcgat tgccacggcc gcggcggcgc gactcgtgct tgccgcccga ctcgacgact
     6241 actgtctcgg caacttgtgc ctcatacatc tgttccgccg attcactgga ttcggccggc
     6301 tcgctcacgt tttcggcacc cgcgcttgca gccgtgcccc gataaacgta cgcgcctgac
     6361 ttctcgtcgc ggccgaactc gagcagcccg cgcgcctgcg cctcttccag cagattgccg
     6421 aacgcgcgaa aaccgtagta tgtctcgttg aaatccggct tacgacgctt gatcgcattc
     6481 ttcaacaccg acgcccagat cttgccactg tcgccacgtt cggatgcgag cgcgtcgaac
     6541 gtttgcaccg cgatctcgac cgctttggtg cgacgctttt cgaggtcttc cttattgcgc
     6601 gacttttctt cgtcgggcgc acgtttggcc gtctgctgcg tggcctgctg cgggcgggac
     6661 gattcgcgtt tggctactgt gcgctgactc tcacgcacga ggtcgtcgta aaagaagaat
     6721 tcatcgcagt ttgcgatcag cagatcggag gtggattgct gcacgccgac gccgatcacc
     6781 tgcttggcgt tctcgcgcag cttcgacacc agcggcgaga agtccgagtc gccactgatg
     6841 atgacgaagg tattgacgtg cgatttcgta tagcagaggt cgagtgcgtc gaccacaagc
     6901 cggatgtcgg cggaattctt gcccgattgg cgcacgtgcg gaatctcgat caactcgaaa
     6961 ttggcctcgt gcatcgcacc tttgaaactc ttgtaacggt cccagtcgca atacgccttc
     7021 ttcacgacaa tgctgccctt gagcagcaga cgctcgagca ccagcttgat gtcgaacttg
     7081 tcgtacttcg cgtcgcgcac gccgagcgca acgttttcga agtcgcaaaa tagcgccatg
     7141 ctgacggttt cactggatga cgccatgaat atctccattg aagcgatcgt caaattcgac
     7201 gaatcgcgac catgatagcg gctcctgcgg aggtgatcag cttttgtgtt cctggcgcac
     7261 cgataggtca ggcgaaaaac cgttcggaac ggaggacttt ggcggcgacg ctcagggccg
     7321 gcggtttgca catgcagcgg gacgaacgga cgcgcgcttg tgcttgtgcg agctccgcgg
     7381 ccctggaagg gtgagagctc aaccgtgcag cgcctgctcg ccgcgaacgc aagcggctgc
     7441 tcgcgatatt ggctcaagac gagatagctc tttacgccgg acacgtatga ggagggcgtc
     7501 cgccggcctc ggcgcgctac atgtcacgat gacgagccgg atcgcgtagc gccgcgcggg
     7561 tttccagcac ctcgtcgacg aggccatacg cctttgcgcc gtgggctgac atgaagttgt
     7621 cgcggtcggt gtcgcgcacg atctcctcga tgctgcgccc ggtgcgctcg gccatcatcg
     7681 cgttgaggcg ttcgcgcagg taaagcacct ctttggcctg gatttcgacg tcggcagcgg
     7741 tgccctgacc gccgcccgac ggttgatgaa tcatgatgcg ggcgttcggc agagcgtacc
     7801 gtttgccgcg ctgaccggcc gtcagcaaaa acgtccccat gctggcggca aagccggtgc
     7861 acaaggtcga cacctcgggc ttgatgaatt gcatcgtgtc gtagatggcg aggccgtcgt
     7921 acaccgagcc gcccggcgag ttgatgtaaa aagaaatgtc cttgtcggga ttttcggatt
     7981 ccagaaacag cagctgcgcg acgatcagac tggctgactg ttcgttcacc ggtccgacca
     8041 gaaaaacgat ccgctcgcgc agcagacgcg aataaatgtc gtaagcgcgc tcgccgcggc
     8101 cggactgctc gatcacggtg ggcacgagcc cgagaccggt gacgcagggg tagctggaat
     8161 gcatgtttgc ctcgtttcag gtggcacggt gccgattagc cgatagtggt ggtgtttcga
     8221 acgacggcgc attcgttgcg ccggttgttg cccatatgac ggaggagagc gaggcaacgt
     8281 gacaggaata tttttgcgtc gggctgtcac attcactcat gctcagtcgt caagctaaaa
     8341 gagatgcaga tgcagcagca cccgcggaca atgttgcacg caggcacgca ggcacgcagg
     8401 cacgcaggca cgcaggcacg caggcacgca ggcacgcagg cacgcaggca cgcaggcacg
     8461 caggcacgca ggcacgcagg cacgcaggca cgcaggcacg ca
//
LOCUS       DATASH010000138         3888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5557777,
            whole genome shotgun sequence.
ACCESSION   DATASH010000138 DATASH010000000
VERSION     DATASH010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5557777"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VHV99_04125"
     CDS             complement(<1..327)
                     /locus_tag="VHV99_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEX3634666.1"
                     /translation="MKRITLVAAVCASFWLLSPVVNAQTQAASTTAANPLPQVDKNFV
                     QAASMSSSTEIDAAKLATSNSSDKDVKSFAHHMMLDHTKLTVQLKMAVPHGVEVPKDN
                     SDTSVLD"
     gene            1273..2577
                     /locus_tag="VHV99_04130"
     CDS             1273..2577
                     /locus_tag="VHV99_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX3634667.1"
                     /translation="MKIALISEHASPLAVAGGVDSGGQNIYVANVARQLLEAGHQVDV
                     FSRRDRALLPLISDMDGVRVIHVPAGPPMQLPKEQLLPFMDEFAAFLIDFFRRESLQY
                     DVMHANFFMSGLAALKVKAALGIPLVTTFHALGRVRRIHQGASDGFPDERFAIEDDLV
                     ALSDVIVAECPQDEADLIQHYHADPARIRIVPCGFDADEFRPIGRPVAREALGWQRDE
                     FIVLQLGRLVPRKGIDNVVRSVGVLKQAFRASARLYVVGGNSDAPNELATPEIARLRG
                     IASECGVAAQVTFVGRRGRAQLRYFYSAADVFVTTPWYEPFGITPVEAMACGTPVIGA
                     DVGGIRYSVADGVTGFLVPPRDPAALAVCLDQLRRDPELAHRMGEAGLARAYAEFTWR
                     GVGESLAQIYARLARATDVAPTEAVVGQMEMPRRAAAGASSG"
     gene            2671..3273
                     /locus_tag="VHV99_04135"
     CDS             2671..3273
                     /locus_tag="VHV99_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HEX3634668.1"
                     /translation="MLRPAVFLDKDGTLLDDVPYNIDPALMRLAPGARDALTLLAVSP
                     FALFVISNQPGVALGMFARSALTEVEAQLHHLFAGCGATLSGAYWCPHHPDGKVAPYN
                     VGCDCRKPGSGLLRRAARAHRIDLGRSWFIGDILDDVEAGNRAGCRTVLLDNGHETEW
                     IEGPLRVPFARAAHLHAAVQFVVRHHSGCRTAPLNAEVTR"
     gene            3270..>3888
                     /locus_tag="VHV99_04140"
     CDS             3270..>3888
                     /locus_tag="VHV99_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013094095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEX3634669.1"
                     /translation="MNAPLLLAQPTLAHARVPLETSPFTPWGDDVRRILCVRLDNLGD
                     VLMTTPALHALRASATDRHITLLASRSGVTLAPFLDDVDDVIEYDAPWVAHASSAVRT
                     LVDDHRMQEHLRRNSFDAAVIFTVYSQSPLPGAMLCYLAGIPRRLAHCRENPYALLTD
                     WLREPEPQQRTRHEVQRQLDLVRQVGAHSPDTRMRFCVHEADRRAL"
BASE COUNT          647 a         1279 c         1233 g          729 t
ORIGIN      
        1 gtcaaggacg ctggtgtccg aattgtcttt cggcacctcg acgccgtgcg gtaccgccat
       61 cttcagctgc acggtcagct tggtatggtc cagcatcatg tgatgagcaa atgacttcac
      121 atccttgtcg ctggaattgc tggtcgcgag cttcgctgcg tcgatttcag tcgacgacga
      181 catgctggca gcctggacga aattcttgtc cacctgaggc agaggattcg ctgcagtagt
      241 ggaagcggcc tgggtctgtg cgttgaccac gggtgaaagc agccaaaaag aagcacatac
      301 ggctgcgacg agtgtaatcc gtttcatgcg tgacctctcc tcaatcggtt gagtgggtta
      361 tgacggccga gggttcgttg cgtctactgg ccggcagcaa ccagcatgcc gggttgtcca
      421 ggtgcacgcc tgatacgcgc gcgggtatct gctttcccgg ccgtcaatgc tcaaggctca
      481 atgcgagtgc gacaccgtgc ttgcttgttc agagagaaca ggctgaacga ctgcgcgcca
      541 gcgcaacagc atgttgccga gatcagcgtc gcggtgcact gggagctgaa cgagcggctt
      601 gcactgccaa gtccctgtcc ttgcgaacgc cgtcttccct ggcgagagtc gcgaggccgg
      661 tcccgacggt gtcagcgctc tatagacggc tcacgcgcca gatgccgggc gtaaagctgc
      721 cgcccgtcgc ggccagcgcc gtctccatcc gacgtgcagc ggactgcgaa ggctccgccg
      781 gcttgtcgcc gtgcccggca ccctgaccca cgcgtgattg ttgtgacgat ccacccgcaa
      841 cccgcactgc ccccggactc gcctgcgcaa tccccgtgac gtacaagttc gcgatcagct
      901 gatataacgc ccccaggcgc cggtcaggcg taaatcgctg ggtacaggga gccattagta
      961 gtgcgtattt cgcgtttatc cgccaacgcg tccctcgcac ccccgtcgtg ctgggagctt
     1021 ttccttgcca gagttacacg ccccgtttgt aaaaacaggg aacgccacag tgcttcgaaa
     1081 aatgcgaagg ggcgcgccag agcgcttcgg gcgtcgcctc aaaagcaatg acgttgtccc
     1141 ctgccgttca ctcgccgttt ggcacaccgc atgctgcggc ccggggaagc cgcgaccgcg
     1201 cagattcgca cagcctgccg gggaagccga cctcgctcaa cgtccgccac tctccacgcc
     1261 gggaagatac ccatgaaaat cgctcttatc agtgaacacg cttcgcctct cgcggtggcc
     1321 gggggcgtcg acagtggcgg acagaacatt tacgtcgcga acgtcgcgcg ccagctgctc
     1381 gaagcgggcc atcaggtcga cgtgttctcg cggcgcgacc gcgcgctgct cccgctcatc
     1441 tccgacatgg atggcgtgcg cgtcatccat gtgcctgccg gtccgccgat gcaattgccg
     1501 aaggaacagc tgctgccgtt catggatgag tttgcagcct ttctcatcga cttctttcgt
     1561 cgcgaaagtt tgcagtacga cgtgatgcac gcaaacttct tcatgtccgg tctcgccgca
     1621 ttgaaggtga aagcggcgct cggcattccg ctcgtcacca cctttcatgc gctcggccgg
     1681 gtgcgacgca ttcatcaggg cgcaagcgac ggctttccgg acgaacgctt cgcgatcgaa
     1741 gacgacctcg ttgcgctgtc ggacgtcatc gtcgccgagt gtccgcagga cgaagcggac
     1801 ctgatccagc actaccatgc cgacccggcg cgtatccgga tcgtcccgtg tggtttcgac
     1861 gccgacgaat tcaggcccat cgggcgccct gtcgctcgcg aagcgctcgg ctggcagcgc
     1921 gacgaattca tcgtcctgca actcggccgg ctcgtgccac gcaaagggat cgacaacgtg
     1981 gtgcgcagcg tgggcgttct gaagcaggct ttccgcgcgt ccgcgagact gtatgtggtg
     2041 ggcggcaact ccgatgcgcc gaacgaactc gcgacccccg aaattgcgcg cctgcgcggt
     2101 attgccagcg aatgcggcgt ggcagcgcaa gtcacgttcg tcggccggcg cggccgtgcg
     2161 caactgcgct acttctacag cgccgccgat gtgttcgtga cgacgccgtg gtacgagccc
     2221 ttcggcatta ccccggtcga ggcaatggct tgcggcacgc cggtgatcgg cgccgacgtc
     2281 ggcgggattc gttattcggt ggccgatggc gtaacgggct ttctggtacc gccgcgcgat
     2341 ccggccgcgc tcgccgtgtg cctggatcag ttgcggcgtg accccgaact ggcgcaccgg
     2401 atgggcgaag cgggtctcgc tcgcgcgtac gcggaattca cctggcgcgg tgtgggcgag
     2461 tcgctcgcgc agatctacgc gcggctcgcg cgggcgacag acgtggcacc gacggaagcc
     2521 gtcgtcgggc agatggagat gcccaggcgc gccgcggcgg gagcgtcgtc cggttaacgc
     2581 tgcgggcaca cacgcagaca gatatcgcgt gatgtcgctt cagcggcacc acttcggcat
     2641 tcactcacgg ttttcgccag gatccccatc atgctgcgac ccgctgtatt tctcgacaag
     2701 gacggcacgc tgctcgacga cgtaccgtac aacatcgacc ccgcgttgat gcgcctggct
     2761 cctggcgcgc gcgacgcgct gacgctgctc gccgtgtcgc cgttcgcgct gttcgtcatc
     2821 agcaatcaac cgggcgtcgc attgggcatg ttcgcgcgct cggcgttgac ggaggtcgaa
     2881 gcccaattgc atcacctgtt cgccgggtgc ggcgcgacct tgagcggcgc gtactggtgc
     2941 ccgcatcatc ccgatggcaa ggtcgcgcca tacaacgtcg gctgtgattg ccgcaagccc
     3001 gggtcgggcc tgttgcggcg cgcggcgcgc gcgcaccgga tcgatctcgg gcgcagctgg
     3061 tttatcggcg acattctcga cgacgtcgaa gcaggcaacc gtgccggctg ccgcaccgtg
     3121 ctgctcgaca acggccacga gacagaatgg atcgaaggcc cgctgcgtgt gccgttcgcg
     3181 cgcgccgccc atttgcacgc agccgtgcag ttcgtcgtgc gccatcacag cggctgccgc
     3241 acagcgccgc tcaacgcgga agtcacgcga tgaacgcgcc actgctgctt gcgcagccga
     3301 cgctggctca tgcgcgtgtc ccgctggaaa catcgccgtt cacgccttgg ggcgacgacg
     3361 tcaggcgcat tctgtgcgtg cggctcgaca atctcggcga cgtgctgatg accacgcccg
     3421 cgctgcacgc gttgcgagcg tccgctacag accgtcatat cacgttgctc gcgtcgcgca
     3481 gcggtgtaac gctggcgcct tttctcgacg atgtcgacga cgtgatcgaa tacgacgcgc
     3541 cctgggtcgc gcatgcgtcg agcgctgtgc gcacgctggt cgacgatcac cggatgcagg
     3601 agcatctgcg gcgcaacagc ttcgacgccg cggtgatttt cacggtatac agccagagcc
     3661 ccctgccggg cgcgatgctc tgctatctgg ctggcattcc gcgccgtctt gcgcattgcc
     3721 gcgaaaaccc ctatgcgttg ctgacggact ggttgcgcga gccggaaccg cagcagcgca
     3781 cgcgtcatga agtgcaacgc cagctcgatc tcgtgcgcca ggtcggcgca cactcgcctg
     3841 atacgcgcat gcgcttctgc gtgcacgagg ccgaccggcg cgcgctcg
//
LOCUS       DATASH010000139         4621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3974783,
            whole genome shotgun sequence.
ACCESSION   DATASH010000139 DATASH010000000
VERSION     DATASH010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4621
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3974783"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..283
                     /locus_tag="VHV99_04145"
     CDS             8..283
                     /locus_tag="VHV99_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004550225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634670.1"
                     /translation="MKKSLLVASLLAAVALAACNKSNDQAAAPASDAAAASAPAAAAS
                     DSAAAASGAAAAASDAAASATGAASDAGAAASDAAASAAAPASGASQ"
     gene            complement(430..2685)
                     /locus_tag="VHV99_04150"
     CDS             complement(430..2685)
                     /locus_tag="VHV99_04150"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEX3634671.1"
                     /translation="MNSPLETSPRPTGGDGAPAAGTPAPLAAASALSASLNPRRGAEL
                     DDIFADNGLLARQITGYRSRASQIEMSRAVAAAMEASGRAMPEPAMFEAQKRPARRLP
                     SPGADAPTEAAESAESNGLDGSENTLIVEAGTGTGKTYAYLVPAMLWGGKVIVSTGTK
                     HLQDQLFQRDIPTVRDALAVPVSVAMLKGRANYLCHYYLQRTADNGRLPSRQETSYLQ
                     DIIRFAKITRTGDKAELASVPETAAVWSMVTSTRENCLGQECPHYKECFVMQARREAQ
                     QADIVVVNHHLFFADIMLRDTGMAELLPTANTIIFDEAHQLPETATLFFGETLSTTQF
                     LELARDSVAEGLGHARESIDWVKLGSTLERAARDVRLAFKEDSVRLSIGQLPDDHPLF
                     PALATLETELDALASALAGQAERAESIGACLRRARELQGVLAGWTTPPTETERAAADA
                     ASADARAERADPNEKVRWIEVFAHTVQLHETPLSVAPIFAKQRAGVPRAWIFTSATLS
                     VRGDFTHYAAQMGLNAKRSMTLPSPFDYPTQGLLYVPRNLPQPSSPMFTDAVFDAALP
                     AIEASGGGVFMLCTTLRAVDRISAKLRDTIEARGWNYPLLVQGDASRTELLDRFRAYG
                     NAILVGSQSFWEGVDVRGDALSLVVIDKLPFAPPDDPVLSARLDALTKKGLSPFAVHQ
                     LPQAVITLKQGAGRLIRAETDRGVLMICDTRLVDKPYGRRIWQSLPPFKRTREIEVVR
                     EFFEENASPAQ"
     gene            complement(2749..2967)
                     /locus_tag="VHV99_04155"
     CDS             complement(2749..2967)
                     /locus_tag="VHV99_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013338815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HEX3634672.1"
                     /translation="MRDQHHVITADTLRDRILQLESEHSGLNRLIERMSDEPGIDDFE
                     LQRLKKRKLKVKDTIILLQLQLEPDARA"
     gene            complement(3104..4072)
                     /locus_tag="VHV99_04160"
     CDS             complement(3104..4072)
                     /locus_tag="VHV99_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634673.1"
                     /translation="MYLASCALCVMTATLAMSAPAVAQSAAPLAAASSLAPAAASGAR
                     AAGTAGAGTANATGAGNAGNAGNAGNAGNAGNADNAASPDSGFDARQKVLDQRSEENN
                     YRYGVAEHDCYSKFFVNHCLSKARDSMRSVQADIRKEQLALDGEQRVQRARERDEQAA
                     LKRAQDEASAPQRAADDARNAQAYEEKQRQHQLDQAKRAAEAPQRNANAQAYQQKLQQ
                     HALDQAQRGVSPSQAAANQQAYDQKQGDFQRKLEEARQQGAQKAQERVEKQQSFEKKQ
                     ADAAQHKADVEARQKQAAEKAEQKRQDQLKQQQELEEQQKQQQQQE"
     gene            4491..>4621
                     /locus_tag="VHV99_04165"
     CDS             4491..>4621
                     /locus_tag="VHV99_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019254711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HEX3634674.1"
                     /translation="MTETVALKIVQRIAAELSVQPRQVAAAVQLLDEGATVPFIARYR
                     "
BASE COUNT          818 a         1489 c         1420 g          894 t
ORIGIN      
        1 cgtgatcatg aagaaatccc tcctcgtagc atccctgttg gcagctgtgg cactggctgc
       61 atgcaacaag agcaacgatc aagccgctgc cccggctagc gacgcagctg ctgcttcggc
      121 accggctgct gcagcttcgg attcggctgc tgctgcatcg ggtgcggctg ctgctgcgag
      181 cgacgctgct gcttcggcta cgggcgctgc ctcggacgct ggcgctgctg cttcggacgc
      241 tgctgcttcg gctgcagctc cggcttcggg cgcaagccag taagctgtcg cgtagggttc
      301 gcctctgggc gaaaactgct ccggaaaatc acctcgatct tccgggcagt aaagcaaaac
      361 gccacggatt cacatccgtg gcgttttgcc atttaaaagc cgttatttca acggcgttca
      421 cttaattatc tactgcgccg ggctcgcatt ctcttcgaag aattcacgca cgacttcgat
      481 ctcacgtgtg cgcttgaagg gtggcaggct ttgccatata cggcgtccat acggtttgtc
      541 gacgagacgc gtatcgcaga tcatcagcac gccgcgatcg gtctcagcgc gaatcagacg
      601 gcctgcgccc tgcttgagcg taatcacggc ctgcggcaac tgatgaaccg cgaacgggct
      661 caatcctttc ttcgtcagcg catccagacg cgccgatagc accgggtcat caggcggcgc
      721 aaacggcagc ttgtcgatca ccaccagcga gagcgcatcg ccgcgcacgt ccacgccttc
      781 ccagaagctc tgactgccca ccagaatcgc attgccgtac gcacggaagc gatcgagcaa
      841 ttcggtgcgg ctcgcatcac cctgcacgag cagcggatag ttccagccgc gcgcttcaat
      901 ggtgtcgcgc aacttggccg aaatgcggtc caccgcgcgc agcgtcgtgc acaacatgaa
      961 tacgccacca cccgatgctt cgatcgccgg caaggcagcg tcgaacaccg cgtcggtaaa
     1021 catcggcgac gaaggctgcg gcaaattgcg cggcacgtac agcagtcctt gcgtcggata
     1081 gtcgaagggg ctcggtagcg tcatcgagcg ttttgcgttc aggcccatct gcgccgcgta
     1141 gtgcgtgaag tcgccgcgca ccgacaaggt agccgatgta aagatccacg cgcgcggcac
     1201 gccggcccgc tgcttcgcga agatcggcgc gaccgacagc ggcgtttcgt gcaactgcac
     1261 cgtatgcgcg aagacttcga tccagcgcac tttttcattc gggtcggcac gttcggccct
     1321 ggcgtccgcc gaagcggcgt cagcggctgc gcgttcggtc tcggtgggtg gtgtggtcca
     1381 gcccgccagc acgccctgca actcgcgcgc gcgacgcaaa cacgcgccaa tcgactcggc
     1441 gcgctcggcc tgaccggcca acgcggaagc caatgcatcg agttcggttt cgagtgtcgc
     1501 aagcgcgggg aacaacggat gatcgtcggg caactgaccg atcgacaagc gcaccgagtc
     1561 ttccttgaag gcgagccgca cgtcacgcgc cgcacgttcg agcgtcgagc cgagcttcac
     1621 ccaatcgatc gattcgcgcg catgacccag gccctccgcc acggaatcgc gtgcgagctc
     1681 gagaaattgc gtggtggaga gtgtctcgcc gaaaaacagc gtcgcggttt ccggcagttg
     1741 atgcgcttcg tcgaagatga tggtgttcgc cgtggggagc aattcagcca tgccggtatc
     1801 gcgcaacatg atgtcggcga aaaacagatg gtgattgacc accacgatgt cggcctgctg
     1861 cgcctcgcgg cgcgcctgca tcacgaagca ctccttgtaa tgcgggcact cctggccaag
     1921 acagttttca cgcgtggacg tgaccatcga ccacaccgcc gccgtttcag gcacgctcgc
     1981 gagttcggcc ttgtcgccgg tgcgcgtgat cttggcgaag cgaatgatgt cctgcagata
     2041 cgaggtttcc tggcgcgacg gcagacggcc gttatccgct gtgcgctgca gatagtaatg
     2101 gcacaggtag ttcgcgcggc ccttgagcat cgccaccgag accggcacgg cgagtgcatc
     2161 gcgcacggtc ggaatgtcgc gctgaaagag ctgatcctgc aaatgcttgg tgcccgtgga
     2221 gacaatcacc ttgccgcccc acaacatggc cgggacgaga tacgcatagg tctttccggt
     2281 gcctgtgccc gcttccacga tcagcgtatt ttcgctgccg tcgagaccat tcgattcggc
     2341 gctttcagcg gcttctgtcg gagcatccgc gcccggcgat ggcagacgac gcgccggacg
     2401 cttctgcgct tcgaacatcg cgggttcggg catcgcgcgg ccggacgctt ccatggccgc
     2461 ggcgacggcg cgcgacatct cgatctgcga cgcgcgcgag cgatagcccg tgatctgacg
     2521 ggcgagcaga ccgttgtccg cgaagatatc gtccagttcg gcgccgcgac gcgggttcaa
     2581 cgacgcggag agcgcagacg cggcagcaag cggcgccggc gtgccggccg ccggagcgcc
     2641 gtcgcccccc gtggggcgtg gtgaagtttc aagcggtgaa ttcaaggcaa gcgtttcctg
     2701 caaagtccgg cgcgcgcggc gcgacccggc acctgccagg ccacgaactc aggcgcgtgc
     2761 gtccggttcg agctgcaatt gcagcaagat gatggtgtcc ttgactttga gtttgcgctt
     2821 tttcaggcgc tgcaactcga agtcgtcgat gccaggctcg tccgacattc gctcgatcaa
     2881 acgattgagc ccactatgct ccgattccag ttgcagaata cggtcgcgaa gggtgtccgc
     2941 ggtgatgacg tgatgttgat cgcgcatgct cgcctcctct cgtaagcggc acgggctgcg
     3001 tcgtgcggcc cgtgccggag gttgctgtcc ggcttaaaac gcgttacagc gactacgata
     3061 ctccgctgcc acaacccggc cagcgacaaa gacagcccag cgactactct tgttgctgtt
     3121 gctgcttctg ctgctcttcg agttcctgct gctgtttcaa ctggtcctgc cgcttctgtt
     3181 cggctttctc cgccgcctgc ttctggcgcg cttccacgtc ggctttatgc tgcgccgcat
     3241 cggcctgctt cttctcaaag ctctgctgtt tctcgacgcg ctcctgcgcc ttctgggcac
     3301 cctgctgacg tgcctcctcg agcttgcgtt ggaagtcgcc ctgcttctga tcgtaagcct
     3361 gctggttagc cgcggcctgc gacgggctga cgccgcgctg cgcctgatcc agcgcatgtt
     3421 gttgcagctt ctgctgatag gcttgcgcgt tggcgttgcg ttgcggcgcc tcggcggcgc
     3481 gcttggcctg gtcgagctga tgctgacgct gcttttcctc gtaggcctgc gcgttacgcg
     3541 cgtcgtcagc ggcgcgttgc ggcgcgctcg cttcgtcttg cgcgcgtttg agcgcggcct
     3601 gctcgtcacg ctcccgggcg cgctgaacac gctgttcgcc gtcgagcgcg agctgttcct
     3661 tgcgaatgtc ggcctgcacg gagcgcatgc tgtcgcgcgc cttgctcaga cagtggttga
     3721 cgaaaaactt actgtagcaa tcgtgttcgg cgacgccata acggtagttg ttttcctcgc
     3781 tgcgctgatc cagaactttc tgccgcgcgt cgaatccgct gtcaggcgat gccgcgttat
     3841 cggcattccc ggcattcccg gcattcccgg cattcccggc attaccggca ttacccgcac
     3901 ccgtagcatt cgcggtgccg gcccctgccg tgcctgctgc ccgcgcgccc gacgcggctg
     3961 ccggcgcgag actgctggcg gccgcgagtg gcgccgcgga ttgcgccacg gccggcgcgg
     4021 acatggctag cgttgcagtc atcacgcaca aagcgcagga cgccagatac atcccattgg
     4081 acgaggacgc tgaccggaac agggagcgtg acgccaatgc gagcgacata ggggacgtaa
     4141 gcgacttaag caacctaaga agcaacctaa gcggcaatcg cgacacattc agcgacagaa
     4201 aagacggcaa tggtgacaaa cgagagacca agcgggagcc caggcggttg agggacaagg
     4261 agatcggcaa cgtcgtaaat ggtcaacgga acatgcccga aattctatca ccctgcggtt
     4321 gagccctttg gcatttatgg caaaatgccc cgctccagca acctggtcgc aacgcgcacg
     4381 cctggcgtgc gccaacgtgt ccaacatgtg cccgacgtgt acccgacgtg cacccgacgt
     4441 gtaccgttag cgcgcgacct tcgattgagt agcaggaaca ccaagacctt atgacggaaa
     4501 ccgtagcact caagatcgta cagcgcatcg ccgccgaact gtccgtccag ccgcgccagg
     4561 tcgcggccgc ggtgcaactt ctcgacgaag gagcgactgt tccgttcatc gcccgctacc
     4621 g
//
LOCUS       DATASH010000140         5053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6744072,
            whole genome shotgun sequence.
ACCESSION   DATASH010000140 DATASH010000000
VERSION     DATASH010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5053
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6744072"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            745..1674
                     /locus_tag="VHV99_04170"
     CDS             745..1674
                     /locus_tag="VHV99_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005254 - chloride channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family ion channel"
                     /protein_id="HEX3634675.1"
                     /translation="MIIRPHLHWFRMLLAWRGSVLPQLLPRLSLIFCIALVAVAAHDH
                     LLPVSLNLNSTAPFSLIGIALAVFLGFRNNASYDRWWEARKLWGQLLNESRSLTRQAL
                     TLQNKQLPKEDIKEFVTALGALPHALRHQLRKSDPHADLAARLPKALFDRVMASRYKP
                     ATLLLFLGEWVQRHAQTGAVDPMAVLAFDRNLNGLSDVIGGCERIVSTPLPFAYSVMI
                     HRTVYFFCASLPFGLVDSIGIFTPVFAVFVAYTFMAHEAIASQIEEPFGTDENDLALN
                     TMSLFIEDATRDLLGEPALEMPKPKENESYILN"
     gene            complement(1795..2535)
                     /locus_tag="VHV99_04175"
     CDS             complement(1795..2535)
                     /locus_tag="VHV99_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HEX3634676.1"
                     /translation="MRMLKSRLGADGRRRAFGALALASAVSVAMLGFAPLAAHAEVAA
                     ADPIDAAMRSCLARSDMSSTVGQVQCMDNARIGWRAALDSAWQQLQTKLPQAQRRPWE
                     KSQASWQASRDVEKQLLAAVFETTRGTMYVLAEADMQLQPVRARALALRSAAARISAG
                     GDPPRRSRACGADAQCEHAMFDLNRYYRRLQAKMPTRSRPTLARAQKAWTAYLDASTP
                     LIDERSRIDIIGARVATLKRLSETVGND"
     gene            complement(2538..4823)
                     /locus_tag="VHV99_04180"
     CDS             complement(2538..4823)
                     /locus_tag="VHV99_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/lysine/ornithine decarboxylase"
                     /protein_id="HEX3634677.1"
                     /translation="MKFRFPVVIIDEDFRSENISGSGIRALAEAIEKEGAEVLGLTSY
                     GDLTSFAQQSSRASCFILSIDDDELLPYVDNVVVEGETPELAAAIVALRAFVTEVRRR
                     NADIPIFLYGETRTSRHLPNDILRELHGFIHMFEDTPEFVARHIIRETKVYLDSLAPP
                     FFKELVQYADEGSYSWHCPGHSGGVAFLKSPLGQMFHQFFGENMLRADVCNAVDELGQ
                     LLDHTGPIAASERNAARIFSADHVFFVTNGTSTSNKIVWHGTVAPGDIVLVDRNCHKS
                     ILHAITMTGAIPVFLTPTRNNFGIIGPIPRSEFEPENIKKKILANPFAREALVKNPEL
                     KPRILTITQSTYDGVIYNVEMIKEMLGDWLDTLHFDEAWLPHAEFHEFYQDMHAIGAG
                     RPRIGAMVFATHSTHKLLAGISQASQIVVQDSKNSRFDKHRFNEAYLMHTSTSPQYAI
                     IASCDVAAAMMEAPGGTALVEESIAEALDFRRAMTKVDAEYGDDWFFKVWGPDQFADE
                     GIGSREDWMLRPNDSWHGFGPLAEGFNMLDPIKATIVTPGLDMDGGFGESGIPAAIVT
                     KYLAEHGIIVEKTGLYSFFIMFTIGITKGRWNSMVTELQQFKDDYDNNQPLWRVLPEF
                     VSHHPMYERIGLRDLCEQIHSVYRANDIARLTTEMYLSSMEPAMKPSDAFAKLAHREI
                     DLVPIDELEGRVTSILLTPYPPGIPLLIPGERFNKTIVNYLRFAREFNERFPGFHTDI
                     HGLVGETINGRIEYFVDCVRA"
     gene            complement(4911..>5053)
                     /gene="dcd"
                     /locus_tag="VHV99_04185"
     CDS             complement(4911..>5053)
                     /gene="dcd"
                     /locus_tag="VHV99_04185"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398615.1"
                     /note="Catalyzes the formation of dUTP from dCTP in
                     thymidylate biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEX3634678.1"
                     /translation="PLPAKIYANEGVAQVLFFESDEICETSYADRGGKYQGQHGVTLP
                     RT"
BASE COUNT         1020 a         1606 c         1482 g          945 t
ORIGIN      
        1 caatcgggtg tttgggcgca tcgccgcaga gcacttcata aagccaatcg ggtgtttggg
       61 cgcattgccg ctgggcactt tcaaaatccg tgagtgttgg gcacgtcggc gaagggcgct
      121 tcataaaagc cgttgggtcc tgggcgcctt ggcgaggcga agccggtagt gcgcgcagcc
      181 cgcaaacaag gcccgtggtc ctatgtggcg ccatctcgcc gaggcgaagc cgacggcccc
      241 gccaagccca acgaagccac tggtttggat gatgagccct tccggcgagc acgcagtgcg
      301 aggcgggaag tatgagccct ttgtcaccaa cgctgaatgt gcgagaacac agattccaga
      361 tgcaccacta ccgcaactgt gcgagggacc cgcttaagaa tttgcggtgt gagccgcttt
      421 cttttgccta cttttctttg cggccggcaa agaaaagtag gtgccgcccc gcacaggggc
      481 gacgctaata gaccactatc aattcaagga aaggccaaaa agccagatca aggagagtca
      541 aatacccaca tcaaggaagg accgccgccg caggcatgca gacagacaga cagacagaca
      601 ataaacgccg cgcaagaaat cagatcaaga aaaaacccac taccgtaggc aaccacccaa
      661 accccacgca ccgcctggcc cccatccaaa aaagcgataa tagaccccac ccgccgaagg
      721 caaaaaaacc cgaagcaacc cccaatgatt atccgcccac atctccattg gttccgcatg
      781 ctgctagcct ggcgaggctc cgtcctcccg caactgctgc cgcggctatc cctgattttc
      841 tgcatcgcgc tcgtcgccgt ggccgcacac gaccacctgc tgccggtctc gctcaacctc
      901 aacagcaccg ccccgttctc gttgataggc atagcgctag cggtcttcct cggtttccgc
      961 aacaacgcca gctacgaccg ttggtgggaa gcccgcaaac tatggggcca attgctgaac
     1021 gaatcgcgct cgctcacccg ccaggcgctc acgctgcaaa ataaacaact gccgaaggaa
     1081 gacatcaagg agttcgtcac cgcgctcggc gcccttcccc acgcgctgcg ccaccagctt
     1141 cgcaagagcg atccgcatgc ggacctcgcg gcgcgcctgc ccaaggcgct gttcgatcgc
     1201 gtcatggcgt cgcgctacaa accggcgaca ctcctgctat ttctcggcga atgggtgcag
     1261 cgtcacgctc aaacaggcgc ggtcgatccg atggcggtgc tcgctttcga tcggaacctg
     1321 aatggtctat cggatgtaat cggcggttgc gaacgcatcg tatcgacgcc gctgcccttc
     1381 gcctactcgg tgatgattca ccgcacggtg tatttcttct gcgcgtccct gccgttcggg
     1441 ctggtcgaca gtatcggcat cttcacgccg gttttcgccg tgttcgtggc ttacaccttc
     1501 atggcgcacg aggcaatcgc ctcacagatc gaagagccct tcggcaccga cgagaacgac
     1561 ctcgcgctca atacgatgtc gctctttatc gaagacgcca cgcgcgacct cctcggcgag
     1621 ccggctttgg aaatgcccaa gcccaaagag aatgaaagct acatactcaa ttgatgacgt
     1681 cgcggcgcaa gcgcggccac gtggtcaggg aagaatggat acggccgggc aggcggtgca
     1741 ccacgcgtac cgttaaacgc cagggctcat tcgttgtcga cgggccgaca accctcagtc
     1801 gttgccgacc gtctccgaca aacgcttgag cgtcgccacc cgcgcgccga tgatatcgat
     1861 gcggctgcgc tcatcgatca acggcgtgct ggcgtcgaga tacgcggtcc aggccttctg
     1921 ggcgcgcgcg agcgtcgggc gcgaacgcgt cggcatcttc gcttgcaagc gacggtaata
     1981 gcggttcaga tcgaacatcg cgtgttcgca ttgcgcatcc gcgccacagg cgcgcgaacg
     2041 ccgcggcggg tctccgccgg cgctgatcct ggccgcggcg ctgcgcagcg ccagcgcgcg
     2101 agcacgcacc ggctgcaatt gcatatcggc ttcggccagc acatacatcg tgccgcgcgt
     2161 tgtctcgaac acggccgcca gcaactgttt ctcgacatcg cgcgaggcct gccagctcgc
     2221 ctggctcttt tcccatggcc tacgttgcgc ctgcggcaac ttcgtctgaa gctgctgcca
     2281 cgcgctgtcc aacgcggccc gccagccaat ccgcgcgttg tccatgcact gcacctgccc
     2341 caccgtcgac gacatgtcgc tgcgcgcaag acaactgcgc atggccgcgt cgatcggatc
     2401 ggcagcggcg acttcggcat gcgccgctag cggcgcgaag ccgagcatcg ccaccgacac
     2461 cgccgacgcc aaggccagcg cgccaaatgc gcgccgccgg ccgtcagcac ccaaccgaga
     2521 cttcaacatc ctcatcgtca ggcgcgcacg caatcgacga aatattcgat gcgcccgtta
     2581 atggtttcgc cgaccagccc atggatatcc gtgtggaagc ccgggaagcg ttcgttgaac
     2641 tcgcgcgcaa accgcagata gttgacgatg gtcttgttga agcgctcgcc cggaatcagg
     2701 agcggaatgc ccggcggata cggcgtcaac agaatcgacg tgacacggcc ttcgagttcg
     2761 tcgatcggta ccaggtcgat ctcgcgatgc gcgagcttgg cgaatgcatc cgacggcttc
     2821 atcgcgggct ccatgctcga caggtacatc tcggtcgtca ggcgcgcaat gtcgttcgcg
     2881 cggtagacgc tgtggatctg ctcgcacaga tcgcgcaggc cgatgcgctc gtacatcgga
     2941 tggtgcgaaa cgaattcagg cagcacacgc cacagcggct ggttgttgtc gtagtcgtcc
     3001 ttgaactgct ggagttcggt gaccatcgaa ttccagcggc ccttggtgat gccgatcgtg
     3061 aacatgatga agaacgaata cagcccggtc ttctcgacga tgatgccgtg ctcggccaga
     3121 tatttcgtga cgatggcagc cggaatgccg gactcgccaa agccgccgtc catatccaga
     3181 cccggcgtga cgatggtcgc cttgatcggg tcgagcatgt tgaagccttc ggcgagcgga
     3241 ccgaagccat gccacgaatc gttcgggcgc agcatccagt cttcgcgcga accgatgcct
     3301 tcgtcggcga actggtccgg gccccacacc ttgaagaacc agtcgtcgcc gtactcggcg
     3361 tcgaccttgg tcatcgcgcg gcggaaatcc aacgcttccg cgatcgactc ttcgaccagc
     3421 gcggtgccgc ccggcgcttc catcattgcc gcagctacgt cgcatgaggc aatgatcgcg
     3481 tactgcgggc tcgtcgacgt atgcatcaga tacgcttcgt taaagcgatg cttatcgaag
     3541 cggctgttct tcgagtcctg cacgacgatc tgcgaagcct gcgaaatacc ggccagcagc
     3601 ttgtgcgtgg agtgcgtggc gaacaccatc gcgccgatgc gcggacggcc cgcgccgatc
     3661 gcgtgcatgt cctgatagaa ctcgtggaac tcggcgtgcg gcagccacgc ttcgtcgaag
     3721 tgcagcgtgt cgagccagtc gccgagcatt tccttgatca tctcgacgtt gtagatcacg
     3781 ccgtcataag tgctctgcgt gatcgtcagg atgcgcggct tcagctccgg attcttcacc
     3841 aacgcttcgc gggcaaacgg attcgcgagg atcttcttct tgatgttttc cggttcgaat
     3901 tcgctgcgcg gaatcggacc gataatgccg aagttgttac gcgtgggcgt gaggaacacc
     3961 ggaatcgcgc cggtcatcgt gatcgcgtgc aggatcgact tgtggcagtt gcggtcaacc
     4021 agcacgatgt cgccaggcgc aaccgtgccg tgccagacaa tcttgttcga ggtcgacgtg
     4081 ccgttcgtca cgaagaacac gtggtcggcg ctgaagatgc gcgccgcgtt gcgctcggag
     4141 gccgcaatcg gaccggtatg gtcgagcagc tgaccgagtt cgtcgacggc attacacacg
     4201 tctgcccgca acatgttctc gccgaagaac tgatggaaca tctggccaag cgggctcttc
     4261 aggaacgcca cgccgcccga gtgcccgggg cagtgccacg agtaggagcc ttcgtccgcg
     4321 tactgcacca gctccttgaa gaacggcggc gcgagcgaat ccagatacac cttggtctcg
     4381 cggatgatgt ggcgcgcgac gaattccggc gtgtcctcga acatgtggat gaagccgtgc
     4441 agttcgcgca ggatgtcgtt cggcagatgg cgcgaggtgc gcgtttcgcc gtacaggaag
     4501 atcggaatgt cggcattgcg gcggcgcact tcagtcacga aggcgcgcaa tgcgacgatc
     4561 gccgcggcca gctccggcgt ttcgccttcc actacaacgt tatcgacgta cggcagcagt
     4621 tcgtcgtcgt cgatcgacag gatgaagcac gacgcgcggc tcgattgctg tgcgaacgaa
     4681 gtcagatcgc cgtagctcgt caacccgagc acttccgcgc cttctttctc gatagcttcg
     4741 gccaaagccc ggatgccgga acccgagata ttctcggagc gaaaatcttc gtcgatgatg
     4801 acgacgggaa aacgaaactt catgggcgat tctccaaaaa gaacgaccgc tgcattctga
     4861 aaattgcagc ggtcacccgg tgtacctggt gggtcagtgc gctgcgaacg tcacgttctc
     4921 ggcaacgtga cgccgtgctg accttgatat ttgccgccgc gatccgcgta cgacgtctca
     4981 caaatctcgt cgctttcgaa aaacagcacc tgagccacgc cttcgttcgc gtaaattttc
     5041 gcaggcaaag gtg
//
LOCUS       DATASH010000141        12290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1601965,
            whole genome shotgun sequence.
ACCESSION   DATASH010000141 DATASH010000000
VERSION     DATASH010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 12290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12290
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1601965"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            365..853
                     /locus_tag="VHV99_04190"
     CDS             365..853
                     /locus_tag="VHV99_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX3634679.1"
                     /translation="MRTVEYRFSTIWRIDAPVQEVWAAIQDSARWPEWWNNVERVDQL
                     EAGSDQGVGLVQRYTWKGWLPYRLVFDMRVTRVEPLVVLEGQASGDVEGAGRWSFSTD
                     GSNLTVVRYDWCVHTNRPWMNALAPLLRPVFQWNHDAVMREGGAALARRLDARLFGIE
                     HG"
     gene            complement(1039..1254)
                     /locus_tag="VHV99_04195"
     CDS             complement(1039..1254)
                     /locus_tag="VHV99_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013094596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634680.1"
                     /translation="MKSIKLRVPREESADLPDDLAAWARITGIDPGIAVISEPAATTD
                     ASSPILYEVYISESFFEQFPEWRMYVE"
     gene            complement(1440..1694)
                     /locus_tag="VHV99_04200"
     CDS             complement(1440..1694)
                     /locus_tag="VHV99_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634681.1"
                     /translation="MYKPLLTRFQYTRKHGKRRTYDVTVNLVQKASGVCAYAAWVHFE
                     AEFKGSGLMLPLVANTPDAAVREAQMRIQKDIDDLVGIVE"
     gene            complement(1753..2043)
                     /locus_tag="VHV99_04205"
     CDS             complement(1753..2043)
                     /locus_tag="VHV99_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012405223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634682.1"
                     /translation="MVLFHRGAAIQASTIRSGNTFVARACILKEDGESTSLGDLGEFA
                     SQTCAYEFAVRCASAFVDGVPMPRSPFRRSSTLDEAEIGNTTLRYPANVPGQ"
     gene            complement(2191..3375)
                     /locus_tag="VHV99_04210"
     CDS             complement(2191..3375)
                     /locus_tag="VHV99_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006410032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3634683.1"
                     /translation="MDRRLLILAVGMFAMGTDNFVVAGILPGVAASLHTSVSLAGLMV
                     SFYALTYAVAAPVMAAVAGAWPRKLLLVSALGIFVAGNMLSALATDLHWVLFSRALSG
                     FGAALFSPTALGVASALAPPEKRGRALAAVTAGLTGATALGSPIGTFIGGFGSWRTTL
                     WFVTLLGMVAMIGVWTMLPSVPRPASIRLRERLAPVRDVRIALTLLTSLFAFGGFLMV
                     YTYAGVVLQRVTHGDGRILAGMFLLWGVAATAGNLLAGRLVDRFESRTIINAMLALAI
                     INFCALPWTSAHAFSAAVALVIWGICGWGLIVPQQHRLVQLTPGVAPLLLALNNTATY
                     IGLACSGVLGGAVLQSIGGQYLSLVAAALIAIALLLAEAAHRCMNVSHVQHRTAANTE
                     PV"
     gene            3565..3950
                     /locus_tag="VHV99_04215"
                     /pseudo
     CDS             3565..3950
                     /locus_tag="VHV99_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013343266.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
     gene            complement(4054..6741)
                     /locus_tag="VHV99_04220"
     CDS             complement(4054..6741)
                     /locus_tag="VHV99_04220"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEX3634684.1"
                     /translation="MVRELANAESAAGNFETAIELMQQAIREVPKSIGNYETLSAIYA
                     SMGDTSNSRIAGRQAQQLMTRIDQSDPPQEWRAPTVEYLILESDGQWRKAEPYIRDSI
                     AAYEGSARKDRTPNWVEKRLLHLSTNMVMQGRYDEAEAIDRQVVHTLLHRVGRTNLDT
                     VDAILALGESVYARNHIADALRLADEALAILDEMKVPPNARQRRLIDHLKGRIYLAEG
                     DLSKSLASFEQAKAGLPDDDFFARSLFQQSPDAILSLAANGQSERALPLASNLVELRR
                     STYGEGSREWVEARAIHAFALVHASQNAAAIADFDVFAQYATTRGGDNVFDHVRYRLL
                     TECYVGMLFDASAQASDTNAKRQMAEQAFVMADSAREGEVRRAIQASSTRAAASSPQS
                     GDIVRQEQDLSLRASALRVHLARLSATQPSPQIQQITATLVDQLKQTQREQDTVHKDL
                     VAHLGERADALESPGVGVSAVAKALHPDEVLVTTFLSSDRTYIWSVDPSGSILFRASS
                     GGRQQIGRLVASVRATTEIHGDTFGSIKPFDFAASHAIYKQVLEPVRPGWEHAHSLVV
                     VPDEALGFVPFALLPVELPQRLTDSQAMFDGYRQVAWLVRTHATSSVPSAAAFVSLRR
                     VSEVTGTRKSFIGFGDPMFSAAQSAKTLQLSQRGAGTIHVLDTRITERGALDSQALVS
                     AHLDDLMPLPETADEVRRVARALGANENSDVLLGAAATVSAAKSALLADRRIVMFATH
                     GLVPGDLDGLEQPALALSNPSVVGGTGDGLLKMDDVLTLKLDADWVVLSACNTAAADG
                     AGSEAVSGLGRAFFYAGTRALLATHWAVETESAQELTTHLFSLQKTDPTLGRAEALRQ
                     TVIAMIDQSGRLRDGKMIASYAHPLFWAPFALFGDGGGN"
     gene            complement(7514..8287)
                     /locus_tag="VHV99_04225"
     CDS             complement(7514..8287)
                     /locus_tag="VHV99_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006494477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX3634685.1"
                     /translation="MDIFRTIEDCHRSPRAIAALASDYPHGYYIAPHRHLRAQLIHAL
                     TGVMTVMSEHGSWIVPAGRAVWMPAGSDHAVRFFSNVSMRTVYVAPDARPSLPGGCEV
                     IEVSPFLREAIVAATRVPLDYAFGGRDHRVMELILDELETAPRLQLHVPMPRDLRLMR
                     LCEQLVASPSAPVTLADLAAGINVSERTLARLFHRELGMSFGQWLRRMRLLLSLPRLA
                     AGVSVLQVALEHGYDSPSAYSAMFRRTLGIAPTAYLNAQ"
     gene            8748..10217
                     /locus_tag="VHV99_04230"
     CDS             8748..10217
                     /locus_tag="VHV99_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3634686.1"
                     /translation="MLLTSLTAGLNEHVTEVAMTDVRGALSIGHDEGTWLTALFEATN
                     VSAMAFAPWCSITFSLRRFTIGATLAFALLGLLCPFAPNVGTLAVLRALQGLAGGCLP
                     PMLMTAALRYLPPNIKLYGLAGYALTATFGPNLGTPLAAFWTEYVNWRMVFWQVVPLC
                     LISSAAIAYGLPQDSLRLERIRSFNWVGLLTGFPAISMLVIGLYQGDRLDWLNSPLIC
                     VMLGGGGLLFCLFLLNEWSHPLPFFKLRMLAQRNFTHSLVTLAGVLVVLLGVAVIPSD
                     YLAEVRGYRPLQTAPLALVVALPQLIALPVTAAILNIERVDCRWVLGLGLTLIATSCV
                     LGSFMTSDWVRENFYLLQAFLTVGEPMAVIPLLMEVTTGLPPTEGPFASAMFNMVKGF
                     AAAVGTALVEGLGTAREHYHSAMLVDRLGSEPLVAQGAARTGMLDDLAGRIHAQAVVL
                     TSADLYLVMAAIAIALVLLVPILPVRVRPPRAATQTTSR"
     gene            10229..11302
                     /locus_tag="VHV99_04235"
     CDS             10229..11302
                     /locus_tag="VHV99_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006052651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEX3634687.1"
                     /translation="MSLPSQPSRKTVLLGAFALLLVGCGWGGTKLVSKPDAETTDDAY
                     VSADYTLVAPRVAGQIAEVLVDDNQVVKAGQLLARIDDRDYRAALASAEGDVKIAMAA
                     VGDFEAQIAQHPALVAQAQATLRSDEAAIAFASANATRYRDLSAGGAGTMEEQQHAVT
                     ALDQQQAGRDRDRAALLAAQQQLDILQAGRDKALGALARAQAALDQAKLNLSYTEIRA
                     PIDGMVGQRSIRVGAFVSAGTPLLAVVPLSQAYVIANFQENQLARMRQHQPVRITVDS
                     FPGLALKGHVDSLAPATGVAFAPIAPDNATGNFTKIVQRLPVKITIDPGQPEAARLRV
                     GLSVEPTVNVAASEAPVEGTEKK"
     gene            11299..>12290
                     /locus_tag="VHV99_04240"
     CDS             11299..>12290
                     /locus_tag="VHV99_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012404252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX3634688.1"
                     /translation="MTVRVLSIRAPLLALLSGAVLAGCTVGPDFQRPKVAAPTDFTRA
                     DHAQAPSRPVEATLAPAWWSLLGDPELVALESRLATDNLDVQAASARLLQSRAGLRVA
                     GAEQFPTIGGAASYNREQASPNGIVSLLGVNPAGAQPESAGGDSAFGVASLPGTSGSP
                     PYNLWQYGFDASWELDLWGHARRGVEAADAAMQASVEDRRATLLSAQAELARDYIELR
                     ATQTLLGITNKNLALARDTVKLTRLRLTEGVTTNLDVANAAAQVASIEARLPPLQTRR
                     DALINALSFLLGEEPGALGQTLADARDIPSLPARAPIGFPSELVRRRPDIRRAEA"
BASE COUNT         2313 a         3823 c         3776 g         2378 t
ORIGIN      
        1 ctctcctcaa gttcgacact agcctgatca gcggcgcgac gcgtaggtcg cggaccaacg
       61 ggcgaagcgt gcagcggcac ctggcggttg tcacgagaga gacttgcgcg gttgcgccag
      121 ttgccggcta cgaatgcgcg cctcgccaga gagaccaggg aatcaccggc gcacggcgga
      181 cacaacctaa ctttctcgca tgcgaccggg tatgtctgta cggtagcagt ccgaatgccc
      241 aaagagagcc aatacgattc gacgcgatat cgagcggtgg aaaccgtgtg ctgtctcgcc
      301 ggtgatgaca agtgcggtga gcatctgaca aaatctctac aacaatacta agaatggagg
      361 cagcttgcgg acggtcgaat accgcttttc caccatttgg cgcatcgatg ccccggtgca
      421 ggaagtctgg gcggcaatcc aggattcggc gcgctggccc gagtggtgga ataacgtcga
      481 gcgcgtggac cagctagagg ccggttccga tcagggtgtg ggcctcgtac agcgctatac
      541 atggaaaggc tggttgccct atcgcctcgt gttcgatatg cgggtgacgc gcgtcgaacc
      601 gttggtcgtg ctggaggggc aagccagtgg cgacgtcgaa ggcgccggtc ggtggagctt
      661 ttcgaccgat ggatccaacc ttaccgtggt tcgctatgac tggtgcgtgc atacgaaccg
      721 cccgtggatg aatgcattgg cgccgctgtt gcgaccggtt ttccaatgga accacgatgc
      781 agtgatgcgc gaaggcggcg cggcgctagc gcggcggctg gacgcgcggc tgtttggcat
      841 cgagcatgga tgaatccggc ggttgccgac actcacacaa cgacaccgat tccgctcgct
      901 acaagagatg agtacctgag tggggaatcg aatttcgacc gttcatgctc gacataccga
      961 acgagttagc cttgctctat ccgccgtacc aggcgcaggc gcgcctcgtc accgcgtttt
     1021 caccaatcgc acggccggtc attctacgta catccgccat tcagggaact gctcgaagaa
     1081 gctctcgctg atgtagacct cgtacaggat gggcgaactc gcatccgtcg tagcggccgg
     1141 ttcgctgatt accgctatcc cgggatcgat gccggtgatt ctcgcccaag ctgccaggtc
     1201 gtcgggaaga tcggctgact cttctcgcgg cacacgcaat ttgatcgatt tcatcgcctt
     1261 gcctcgcaac tggaagttcg cgttgggatc gctaccgggg gtggcccgcg gcggaggttc
     1321 gctgcccggc cgctacagct gattccaccg gcaggagaat acattcgccg cgaacgcgga
     1381 ttctggttca ccgtcgtaac ggtcttccag accgaccacg atcacacctg ttattgcctt
     1441 tactcgacga tgccgaccag atcgtcaatg tccttctgga tacgcatctg tgcctctcgt
     1501 acggcggcat caggtgtatt ggcaacgagt gggagcatca accctgaacc cttgaattcg
     1561 gcttcgaaat gcacccatgc tgcgtacgcg caaacaccgg aagccttttg cacgaggttc
     1621 accgttacgt cgtatgtgcg tcgcttgcca tgcttgcggg tgtactgaaa gcgggttaac
     1681 agtggtttgt acatgacgtt taccttggct tggccgctgc tggtttgacc tgttagctga
     1741 ggaggcagtc gttcactgcc cagggacgtt tgccggatag cgcagcgtgg tgttacctat
     1801 ttctgcctcg tccaaggttg atgatctgcg gaacggcgag cgaggcatcg gcacaccatc
     1861 cacaaaagca ctcgcacacc gcacggcaaa ctcgtatgca caggtctggc ttgcgaactc
     1921 accaaggtcg cccaatgagg tggattcgcc gtcttccttc aaaatacagg cgcgggcgac
     1981 gaacgtgttt ccggacctta tcgtcgacgc ctggatcgct gcgccgcggt gaaatagaac
     2041 cattgcatgc cctccgatca cagaatatgg aaggacattg taatgttgtc attttcgcga
     2101 gtggtgactg ttacctttcc acataagccg cttaagtgtg acgaaagcga cgcggacgct
     2161 ggcgatattg actgcagtgg ttcgtaccga tcagacgggc tcggtattgg ccgccgtgcg
     2221 atgctgtacg tgcgacacgt tcatgcaacg atgtgcggct tcggcgagca acaaggcaat
     2281 cgcgatcagc gccgcggcca cgagactcag gtactgcccg ccgatcgact gaagcactgc
     2341 tccgccaagc acaccggagc aggcgagtcc gatatacgtt gcggtattgt tgagagccag
     2401 cagcaggggc gccacccccg gcgtgagttg caccagccga tgttgttgcg ggacgatgag
     2461 cccccatccg catatgcccc agatcacgag cgcaaccgca gcactgaatg catgcgcgga
     2521 cgtccacggc aacgcacaaa aattgatgat ggcgagcgcc agcatggcgt tgatgatggt
     2581 tcggctttcg aagcggtcga caaggcggcc ggccagaaga ttcccggccg tcgccgccac
     2641 gccccacagc agaaacatcc cggcgagaat gcgtccgtcg ccgtgcgtta cacgttgcag
     2701 cactacgcct gcatacgtgt acaccatgag aaacccgccg aaggcgaata gcgaagtgag
     2761 caaggttagc gctatgcgca catcgcggac cggtgcaagc cgttctcgca acctgatgga
     2821 cgccggccgg ggcacggatg gcagcatcgt ccagacgccg atcatcgcca ccatcccgag
     2881 caaggtgacg aaccagagag tcgttctcca gctcccgaat ccgccgataa acgtgccgat
     2941 cggtgagccg agggccgtcg ctcccgtcaa tccggccgtt actgcggcca acgcacggcc
     3001 gcgtttctca ggtggtgcaa gcgcagaagc aacgcccaat gcagtaggcg agaacagagc
     3061 cgcgccgaat cccgacaaag cccggctgaa caaaacccag tgcaagtccg tggcgagcgc
     3121 gctgagcatg ttgccggcaa cgaaaatgcc gagagcggaa accagcagaa gcttgcgcgg
     3181 ccaagcgccc gccacagccg ccatgactgg ggcagccacc gcgtaggtca acgcgtaaaa
     3241 gctgaccatc aaccccgcca ggctgacgga cgtatgcagt gatgcagcga cgcctggaag
     3301 aataccggcg accacgaagt tgtcggtacc cattgcgaac atgcccacgg ccaatatcag
     3361 gagtcgacga tccatatgaa ggttctcgcg ataagcggtt tgaggttcaa tccgaaggcg
     3421 cgcgtcgcaa tttgactggt gtgcgatcgc ggcgtcgggc cggccagttt ttgcgctgga
     3481 acgatatggg taaagaataa tcgtcgacga ccacgtctca aatgtcatat atacacgcga
     3541 ttcaggacat gacctgggca agctatgcac acgattggct tcgttgcatt cccgaagttc
     3601 tacctgatgg gtttcgccgc agtcactgcc tttgaatggc gaacgtcgtg ctcggcgagc
     3661 cggcatgcca ggtgacagtc gtgcctgaag agggaggact cgtattgtcc tcggcaggta
     3721 ttcgcgtgaa aacgcagccc ttctgcgacg ccgcattcga cacagtcatg tttggctctg
     3781 gcgtcgagat cgacattgcc tcggccggat tgactgccgt cgtcaggcgc gctttgagaa
     3841 cgtcgaggcg gatcgcggcg ccgtgcacag acgcatttat cctggccgaa gcaggcgttc
     3901 tggacggtcg ccgggcgacg atacattggc gcttcgcaca cgatctgcag tgcagcttcc
     3961 acaactcagg acgttcgtcg tgggaccggg tccggtcagt gtccgtcacc gggaggaact
     4021 aacagtaacc gccagaccga tgatacagac gtcttagttt cctcctccgt ctccaaagag
     4081 tgcgaacggt gcccagaaaa gcgggtgcgc atacgacgcg atcatcttgc cgtctctcaa
     4141 tcgtccggat tggtcgatca tcgcaattac ggtttgccgc aatgcttctg cgcgtcccag
     4201 cgtcggatcc gttttttgaa gagagaacag atgggttgtt agttcctggg ccgattcggt
     4261 ttctactgcc caatgcgttg caagcagagc acgcgtgccc gcgtagaaaa aagcccgccc
     4321 gagacctgac actgcctcgg aaccggcacc atcggccgcc gcggtattgc aggccgacaa
     4381 cacaacccaa tcggcgtcga gtttgagcgt gagcacatcg tccatcttga gcagcccgtc
     4441 tcccgtgcca cccaccacgc ttgggttgga caaggccaga gcaggttgct cgagcccgtc
     4501 gaggtcgcct ggcaccagtc cgtgcgtggc aaacatgacg atacggcgat cggcgagcaa
     4561 cgcgctcttc gccgcggata ccgtcgcagc cgcgccgagc aggacgtccg agttttcatt
     4621 cgcaccaagc gcacgggcga cgcggcgcac ctcgtcggcc gtttccggga gaggcatcag
     4681 atcgtccaga tgcgccgaga caagcgcctg gctgtccagt gcgccacgct cggtgatacg
     4741 tgtatcaagt acatggatgg tgcccgcacc gcgttggctc aattgaagcg ttttggctga
     4801 ctgagcggcg ctaaacatgg ggtcaccgaa gccgataaaa ctcttgcggg tgcctgtcac
     4861 ctccgatact cgccgcagac tgacgaaggc cgctgccgac ggcacgctcg aagtggcatg
     4921 cgtgcggacc agccatgcaa cctgccgata accgtcgaac atcgcctgac tgtcggtcag
     4981 acgctgcggc agttccacgg gcagtagcgc aaacggcacg aaaccaagcg cttcgtcagg
     5041 cacgacgaca agcgaatgcg cgtgctccca tcctggtcgt acaggttcga gcacctgttt
     5101 gtagatggcg tggctcgcgg cgaaatcaaa cggcttgatg cttccgaagg tatcgccatg
     5161 tatttcggtc gtcgcccgga ccgacgccac aagccggcca atctgctgac ggccgccgct
     5221 ggatgcacga aacaggatcg agccggaagg atcgacactc cagatgtagg tgcgatcgct
     5281 gctgaggaat gtcgtgacca gcacttcgtc ggggtgcagt gccttcgcga cggcgcttac
     5341 gcccacgcct ggcgattcca gcgcgtcggc gcgttcgccc aggtgcgcga ccagatcctt
     5401 gtggacagtg tcctgttcgc gctgggtctg cttcaattgg tcgacgagcg ttgccgtgat
     5461 ctgctggatc tgcgggctcg gttgagtagc tgacagcctc gcgagatgaa cgcgcaatgc
     5521 actcgcgcgc aaactgagat cctgttcctg acgaacgatg tctccggatt gtggcgacga
     5581 agcggcagct cgagtggacg aagcctgaat cgctcgacgc acctcacctt cacgtgccga
     5641 atccgccatg acaaaagctt gctcggccat ctgccgcttc gcgttggtat cgctcgcctg
     5701 cgcggatgcg tcgaacagca tgccgacgta gcattccgtc aggagtcggt agcgtacatg
     5761 gtcaaatacg ttatcgcccc cacgggtcgt ggcgtattgc gcaaatacgt caaaatccgc
     5821 gatcgctgcg gcattctgac tggcatgcac gagtgcgaat gcgtgaatcg cgcgtgcctc
     5881 gacccattcg cgactgccct cgccatacgt gctgcgccgc agttccacca ggttactcgc
     5941 gagcggaagc gcccgctcgg actgaccatt cgcagccaga gacagaatcg cgtccggcga
     6001 ctgctgaaag agcgacctcg cgaaaaagtc gtcgtcgggc aaccccgctt ttgcctgctc
     6061 aaatgatgcg aggcttttgg acaagtcacc ttccgccaga tagattcgcc ccttcaaatg
     6121 atcgatcagg cggcgttgac gtgcatttgg cggcaccttc atctcgtcca gaatcgcgag
     6181 ggcttcgtca gcaagacgaa gcgcatcggc gatgtgattg cgggcataaa ccgactcgcc
     6241 cagtgcaaga atggcatcga cggtatcaag gttggttcgc cccactcgat gaagcagcgt
     6301 gtgaacaacc tggcggtcta ttgcctcggc ctcgtcatat cgcccctgca tcaccatatt
     6361 cgtggaaaga tggagcaacc gtttctcaac ccagttgggc gtccggtctt tgcgagcgct
     6421 gccctcatac gcggcaatcg agtcacgtat atagggctcg gctttgcgcc actgcccgtc
     6481 cgactccagt atgagatatt cgactgtcgg ggcgcgccac tcctgtggcg gatcggattg
     6541 gtcgatgcga gtcataagtt gctgggcttg acgacccgca atgcgagagt tgctcgtgtc
     6601 gcccatcgat gcatagatgg cactcagcgt ttcatagttg ccgattgatt tcggtacctc
     6661 gcgtatggcc tgttgcataa gctcaatggc cgtttcgaaa ttgccggccg ctgattcggc
     6721 attagcaagt tcgcgtacca cgtcacgtcg cgtacctgtg acgcgagtgt tcgtcgtttg
     6781 cccataggcg gtacgcagat cggcgagcgc ctgttcggga cggccaatct gttcggctgc
     6841 acccgcgcgt ttccaataga aggtcacgac actttgacct tcctgcggcg cgcttgcgtc
     6901 ggccatagcc gtgagacgct gcagcgtggc ctcgttgccc ttcacggtgc ggatttccgt
     6961 gagaatgtcg ttcgtattcc gcggcggctg caggattggc tgagcggacg tcgagagcgg
     7021 caactttgcc gctccgtcct gacgtgtcag cgtgccggac gagcaagcgg caagcagcaa
     7081 cgtggcaaac caccacgcta ggcgcacggt cgatttcgct ttcactcgcc tcccttggcc
     7141 gaagcattgc caaatcttca gccttattct gtgcgtatga cggtccgttc tggttacacg
     7201 gctcattccc gcctgtgacg cgtcgatcca gtgtagtcac agaagactaa aacacgaaag
     7261 cgcttagcga acgttagcca agctgacaat ttcacaagga acgttctcaa cctccgcacc
     7321 catgcgggaa agcacgtgat gcgtacacgt gctcttctct atgtcctgaa ccattgcgtc
     7381 ggaacgatcc cgccctcaac gcaacaaaat agtgtcacga cacacggcct cagccgtgtt
     7441 cagaccgacg gcgtcggctt tgtgtccgga gcagctcgga agaataggtg ggtgatgcgc
     7501 attcaatggt cagctattgc gcgttcaggt aggcagttgg cgcgatgccg agcgtgcggc
     7561 ggaacatcgc ggagtaggcg ctgggactgt catagccatg ctccagcgcg acctgcaaca
     7621 ccgacacgcc cgccgcgaga cgtggcaggc ttagcaacag gcgcatgcgg cgcagccact
     7681 gcccgaagct cattccgagt tcgcgatgga acaggcgggc gagcgttcgc tcgctaacgt
     7741 tgatcccggc ggcaagatct gcgagtgtca ccggtgcgga agggcttgcg accagctgct
     7801 cgcacagacg catgagccgc aggtctcggg gcataggcac atgcaactgc agccgtggcg
     7861 ctgtctcgag ctcgtcgaga atcagctcca tgacgcggtg atccctaccg ccgaacgcgt
     7921 agtccagcgg tacacgtgtc gcggcgacga ttgcttcgcg cagaaacggc gatacttcaa
     7981 tgacctcaca gccgcccggg aggctggggc gggcgtcagg ggccacatag accgtgcgca
     8041 tgctgacgtt gctgaagaaa cgcaccgcat ggtcggagcc ggctggcatc cacacggcgc
     8101 ggcccgccgg cacgatccaa ctcccgtgtt cgctcatgac cgtcatcacg ccggtcagcg
     8161 catgaatcaa ctgcgcccgc aggtggcggt gcggagcgat gtaatagcca tgcggataat
     8221 cacttgccag ggccgcaatc gcgcggggcg agcggtggca atcctcgatg gttcgaaaaa
     8281 tgtccatagt acgacgaatg tagctagtcc gacgtcggat taactaagta aaaaatggtt
     8341 agagggctgt ccgtattgcg acgaacgttg gcatttttga acgggatggc cgaggctgga
     8401 gttgctaaag taagggtaat ccactacaca cgctgagtat aagccgtgcc gagttcgcgc
     8461 cgaaatttgt tcgccaccat ctggtccctg tttcagccta tctgtggcgg cgtcgggccc
     8521 atggcaacgc tcggcgtcac caacggccct atggcggccg gccaatgcgt ggcggccacg
     8581 gcttcgttcg catttgctgc tccctcggta tgacgcccgc cgaagttttg gcgcccgcgc
     8641 cgagccagcg ggcgcctgtg gcgccaatcg cgtctgtggc gccaatcgcg cctgctccgc
     8701 ccgcgccacc cgcttttggg cttcgcattg cgatcgggct gctgggcatg ctgctcactt
     8761 cgctcacggc cgggctgaac gagcatgtca ccgaagtggc gatgacggac gtgcgcggcg
     8821 cgctctcgat cggccacgac gagggaacgt ggctgacggc gctctttgag gccaccaacg
     8881 tctctgccat ggcgttcgcg ccctggtgct cgatcacctt ttcactgcgg cgtttcacta
     8941 tcggcgcgac gctggcgttt gcgctgctgg gcctgctttg cccgttcgcc cccaatgtcg
     9001 ggacgctcgc tgtcctgagg gcgctgcagg ggctggccgg aggctgcctg ccgccgatgc
     9061 tgatgaccgc agcgctgcgc tacctgccgc ccaacatcaa gctttatgga ctcgccggct
     9121 atgcgctcac cgctacgttc ggacccaacc tggggacgcc cttagccgct ttctggaccg
     9181 aatacgtcaa ttggcgcatg gtgttctggc aggtggtgcc cctttgcctc atcagcagcg
     9241 cggccatcgc ctatggcctg ccgcaggatt cgctcaggct ggaacgcatc cgctcgttca
     9301 actgggttgg cttgctcacc ggctttcccg ccatctcgat gctggtgatc gggctgtacc
     9361 agggcgaccg gctggattgg ctgaactcgc cgctgatctg cgtaatgctc ggtggaggag
     9421 gcctgctgtt ctgcctgttc ctgttgaacg aatggtcgca cccgctgcct ttcttcaagc
     9481 tgcggatgtt ggcccaacgc aatttcacgc attcgctggt cacgcttgcc ggcgtgctgg
     9541 tggttctgct gggcgtggcc gtcattccca gtgactatct cgccgaagtg cgcggctacc
     9601 ggccgctgca gaccgcgccg ctggcgctgg tggtggccct gcctcaactc atcgcgttgc
     9661 ctgttacggc ggcgatcctc aacatcgaac gcgtcgattg ccgctgggtg ctggggcttg
     9721 gtctcacact tatagccacc tcttgcgtgc tcggcagctt catgacctcg gactgggtgc
     9781 gcgagaactt ctacttgctc caggcgtttc tgacggtcgg cgagccgatg gccgtgatcc
     9841 cgctgctgat ggaagtgact accggcctgc cgccaactga gggcccattc gcctcggcca
     9901 tgttcaacat ggtgaagggc tttgccgcgg cggtcggcac cgccctggtg gaagggctgg
     9961 gtaccgcgcg cgagcactac cattccgcga tgctcgtcga ccggctgggc agcgaaccgt
    10021 tggtcgccca aggcgccgcc cgcacgggga tgcttgacga tctggccggg cggattcacg
    10081 cgcaggcggt cgtgctcacg tcggccgacc tctatctcgt catggcggcc attgccatcg
    10141 cacttgtgct gctcgttccc atcctgcctg tgcgcgttcg tccgccccgc gcagccacgc
    10201 agacaacttc ccgctgaacg cttcgaacat gtcattacca agtcaaccat cccgaaagac
    10261 ggtcttgctg ggggccttcg ccctgctatt ggtaggttgc ggctggggtg gcaccaagct
    10321 ggtctccaaa cccgacgccg aaacaaccga cgacgcctac gtcagcgccg attacacgct
    10381 cgtcgcgcca cgcgtagccg gccagattgc cgaagtgctg gtcgatgaca atcaggtggt
    10441 gaaggcgggc cagcttctcg cccgcattga tgaccgggat tatcgcgccg cgcttgccag
    10501 cgcagagggg gacgtgaaga tagcgatggc cgctgtgggt gatttcgaag cgcagattgc
    10561 gcaacacccc gcgctcgtcg cacaggcgca agcaaccttg cgctccgacg aggccgccat
    10621 cgccttcgcg agcgccaatg ccacgcgcta ccgcgatctc tcagcgggcg gtgccggcac
    10681 gatggaggag caacagcacg cggtgaccgc gctcgaccag cagcaagccg gccgggaccg
    10741 cgaccgcgcc gcgctgcttg ctgcccagca gcagctcgac atcctgcagg cggggcgcga
    10801 caaggcgctc ggtgcgttgg cacgcgcgca ggccgcgctc gatcaggcga agctcaacct
    10861 ctcctatacg gaaattcgcg cgccgatcga cggtatggtc ggccaacgct cgatccgcgt
    10921 gggcgcattc gttagcgcgg gcaccccatt gctggccgtg gtgcccttgt cgcaggcgta
    10981 tgtcattgcc aattttcagg agaatcagct tgcccgtatg cgccagcatc agccggtgcg
    11041 catcaccgtg gacagctttc cgggcctcgc cctgaagggc catgtcgaca gccttgcgcc
    11101 ggcgacgggc gtcgcgttcg cgccgattgc gcccgacaac gccaccggca acttcaccaa
    11161 gatcgtccaa cgcctgccgg tcaagatcac catcgatcca ggacaacccg aggcggcgcg
    11221 cttgcgcgtg ggtctttcgg tcgagccgac tgtcaatgtc gccgcaagcg aagcaccggt
    11281 cgaagggacg gagaaaaagt gacggtgcgc gttctttcca ttcgggctcc tctgctcgcc
    11341 cttctgtcgg gtgcagtgct tgccggctgc acggtcggac cggacttcca gcggcccaag
    11401 gttgcggcgc cgactgattt cacccgcgcc gaccacgccc aggcgccgag ccggcccgta
    11461 gaagcaacgc tcgcgcccgc gtggtggtcc ttgttgggcg accctgagct ggtcgcgttg
    11521 gagtcgcgcc tcgccactga caatctcgac gtgcaggccg cctcggcgcg gctcctgcaa
    11581 agccgcgccg ggttgcgcgt cgccggtgcg gagcagtttc cgacgatagg cggtgcggcg
    11641 tcctacaatc gcgagcaggc aagcccgaat ggcattgttt cgttgctcgg cgtcaatcct
    11701 gccggagcac agcccgaatc cgcgggcggc gactccgcgt tcggtgtcgc gagcttgccg
    11761 ggcacgagcg gatcaccgcc ctacaacctg tggcaatacg gcttcgacgc gtcctgggag
    11821 ttggacctgt ggggtcacgc acggcgcggc gtggaagcag ccgacgcggc catgcaggca
    11881 tcggttgagg accgccgcgc taccttgctt tccgcccagg ccgagcttgc ccgcgactat
    11941 atcgagttgc gcgccacgca gaccctgctc ggcatcacga acaagaacct cgcgctagcc
    12001 cgcgacacgg tcaagctgac ccgcctgcgg ctgaccgagg gcgtcacgac caacctcgac
    12061 gtcgccaacg cagcggcgca ggttgcgtcg atcgaagcgc ggctgccgcc gctgcaaacg
    12121 cgccgcgatg cgctgatcaa cgcactaagc tttctgctcg gcgaagaacc cggtgcgctc
    12181 ggccagacgt tggcggacgc gcgcgacatt ccgtccctgc cggcccgcgc gccgatcggc
    12241 tttccgtcgg aacttgtacg acggcggcca gatatccggc gggcggaagc
//
LOCUS       DATASH010000142        10665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1997767,
            whole genome shotgun sequence.
ACCESSION   DATASH010000142 DATASH010000000
VERSION     DATASH010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 10665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10665
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1997767"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VHV99_04245"
     CDS             complement(<1..423)
                     /locus_tag="VHV99_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HEX3634689.1"
                     /translation="MSWPRLVLVPLLVLSAALSVTWILWDHERQAARHELLTQFDFSL
                     GDAVSRVEQRMATYELLLRGAQSLFVATGEVDHDQFRNYIGTLNLDANFSGIQTIGIV
                     EWLPAAQKDVHVAAMRRQGLAGYTIEPKGPREDYAPIIQ"
     gene            complement(420..1214)
                     /locus_tag="VHV99_04250"
     CDS             complement(420..1214)
                     /locus_tag="VHV99_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX3634690.1"
                     /translation="MIRHGLGGLLLALCFVGKAMAAGPDCSRSFTLALHDHGLLYSVD
                     TDSGIDKDFADELIRRSGCQIRVSLMSRARIWKLIESGALDFSLSGIANDERIQYADF
                     AWYFSDKYYLLVRKDAGIHQLSDFEHSDQFQLGVIRSFRYSDSANRLVDTLSEENRVT
                     QAGGLGPLYEALILRRIQGMIIEPFDYPVLDEKKIRDVTTIVEFNDPAVPHGLIMSKR
                     ALSAAERAKWRALVNEMRADGTVRRIFEKYFKPDLAASMVDFQTPP"
     gene            complement(1468..1746)
                     /locus_tag="VHV99_04255"
                     /pseudo
     CDS             complement(1468..1746)
                     /locus_tag="VHV99_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014194036.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            complement(1932..2669)
                     /gene="msrA"
                     /locus_tag="VHV99_04260"
     CDS             complement(1932..2669)
                     /gene="msrA"
                     /locus_tag="VHV99_04260"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX3634691.1"
                     /translation="MHTTSTRQGSRNKRSALVRLAGCVAIAASVFAAQRMANSAEAAV
                     KVPPPAQDEQVGTAHSETAVFAGGCFWGVQGVFEHVRGVKQVASGYTGGAAGTAQYET
                     VSEGDTGHAESVQITYDPTQITYGRLLQIFFSVAHDPTELNYQGPDHGTQYRSAIFPA
                     NAQQRSVAQAYIAQLDKAHVFSGPIVTRIEDFKGFYPAENYHQNFLALHPDYPYIAIN
                     DLPKVSELKRLFPDLYRNDPVLLKVGA"
     gene            complement(2717..4603)
                     /locus_tag="VHV99_04265"
     CDS             complement(2717..4603)
                     /locus_tag="VHV99_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006498597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein DipZ"
                     /protein_id="HEX3634692.1"
                     /translation="MLLIVLAYLGGALTILSPCILPVLPFVFARADQPFVRSGLPLLA
                     GMALTFAVVATLAAVGGGWATQANQYGRWLAIALLAVFGLSLLFPRFADHLMRPLVSA
                     GNRLSNFAQTDGQQGRPGSSFLLGIATGLLWAPCAGPILGLVLTGAALRGASVGTTLL
                     LVAYAAGAATSLAVALLIGGRVFTAMKRSLGAGEWIRRGIGAAMLCGVVAIALGLDTG
                     VLTRISTVATGGIEQKLVDKLSPGATPVNPVAAGSSANASANALASASASDAVMTANP
                     AADTSASAGSGAMMRTAQGAPAGEPAPLPIEGVLPTLDGAVQWLNSPPLTVQGLRGKV
                     VLVDFWTYSCINCLRSLPYVKAWAQKYKDQGLVVIGVHAPEFAFERNIDNVKKATHDL
                     GVDYPVAIDNNYAIWRALNNQYWPAHYFVDAKGQIRYHHFGEGDYAESEKVIQQLLAE
                     AGHAGASKVALGIAGTSAQGVQAAADNADMQSPETYIGYERAENFASPGGEAQDKVHT
                     YAAPSQPAVNDWGLAGAWNVGAEHATLAAPSGRIVYRFHARDLHLVLGPGKDGKPVRF
                     RVSVDGAAPGAAHGTDVATDGSGTVTEQRLYQLVRQTGDVADHTFSIEFLDPGVQAYA
                     FTFG"
     gene            4802..5557
                     /locus_tag="VHV99_04270"
     CDS             4802..5557
                     /locus_tag="VHV99_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3634693.1"
                     /translation="MDHPKRILIVEDDVDIANLLSLHLRDERYEVVHSADGNEGLRLL
                     EQGNWDALILDLMLPGVDGLEICRRARAMTRYTPIIITSARSSEVHRILGLELGADDY
                     LAKPFSVLELVARVKALLRRVEAMAKDSRIDAGSLRIAGLTIDPLTREASVDGKAIEL
                     TPREFDLLYYFAQHPGKVFSRMDLLNAVWGYQHEGYEHTVNTHINRLRAKIEADPAQP
                     ERILTVWGRGYKLAVPGIPGDGPASNSAPRDTP"
     gene            5554..7047
                     /locus_tag="VHV99_04275"
     CDS             5554..7047
                     /locus_tag="VHV99_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX3634694.1"
                     /translation="MTLTLTQRLSLVFSALLLACCGASAWLQIRSSDLHEKEVVQSLS
                     RSLADHIAHRTTLMDANGLRPDAVRQLFGQLMMVNPSVEVYLLDNQGRIQGDDAPAGH
                     VKRQQVDLAPIRQFIAGDALPILGDDPRSLDGKKVFSAAPLQLSGRQPSGYIYVVLLG
                     EAHDALAARVAASSVLRTTLWSMALVALLGLLAGLTAFGLITRPLRRLTDAMRRFDAD
                     GEPDTQPAVPRSAPAGQRDEIAVLETTFAQMANRIGEQWRALTRQDQQRRELIANISH
                     DLRTPLTSLHGYLETLSLKADTLGETERKRYLAIALAQSVKVGRLAQSLFELARLEHG
                     NVQPALEQFSLVDLVQDIFQKFELPAEARHIQLRAEIPPRLPAVSADLGMIERVLTNL
                     LDNAIRHTPAGGVIDVELAHKDGKVSVTVSDTGTGIPAEQREGLFERPFSSAGAHRGG
                     GLGLLIVQRMLQLHQSQIRLIERAGPGTTFCFDLPTTGKSTAAGIPL"
     gene            7217..7345
                     /locus_tag="VHV99_04280"
                     /pseudo
     CDS             7217..7345
                     /locus_tag="VHV99_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176590.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            complement(7475..7789)
                     /locus_tag="VHV99_04285"
     CDS             complement(7475..7789)
                     /locus_tag="VHV99_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006481895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEX3634695.1"
                     /translation="MSWFIYEVPEFNEDGARIEPFSDLRSRVLKVSGQAMADELESVR
                     ANAATTADTPAHPLRAAENPHVFPIPYADSMILVGFIFELKRESRHLVVSPVEMPWLK
                     DA"
     gene            8080..8784
                     /locus_tag="VHV99_04290"
     CDS             8080..8784
                     /locus_tag="VHV99_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013691450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634696.1"
                     /translation="MLTCYFQSGDAIRSRRVSDIVLSGTLDVPAPSARLLADWERETS
                     LHMVLEPGDVEVMPLARARVRWPDYTRCVQAMSDWTCALGLPEVLAASDVALMACRGA
                     RYHHDGAQYGDSAFCNLFMSEDKGLDLHFPSTGQRIPLTRGTAVIFDTGQPHGVVQRG
                     SSGFNAAEFTSAQDCVQIFLTWELPIEDPHVGQALNVAFDIAPAASSQPDEEQVLLNG
                     ERVIVCPDSGRWRPVD"
     gene            9087..9407
                     /locus_tag="VHV99_04295"
     CDS             9087..9407
                     /locus_tag="VHV99_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011658970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HEX3634697.1"
                     /translation="MTAATQFDQVSVIKRANVYFDGKCVSHTVLFADGTRKTLGVILP
                     GTLNFGTDAPELMEVQAGQCRIRLEGSDEWKTYGAGESFSVPGKSRFDIDVTETLDYV
                     CSYL"
     gene            complement(9523..>10665)
                     /locus_tag="VHV99_04300"
     CDS             complement(9523..>10665)
                     /locus_tag="VHV99_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX3634698.1"
                     /translation="TFTIGRGNEICVTAVEALTPLVVGTTLEDIAANMGAFWRALTSD
                     SQLRWIGPDKGAIHLATAAVVNAAWDLWAKAEGKPVWTLLVDMSPEALVRCLDFRYVT
                     DAITPQEAIAMLHRHAGTRGERETEMLAHGYPAYTTSAGWLGYDDDKIRRLAREGVAQ
                     GWTHFKQKVGGNLEEDMRRARILREEIGENRKLMMDANQVWDVDEAVANMRRLAEFDP
                     WWIEEPTSPDDILGHAAIRQRISPIGVATGEHCQNRVMFKQLLQAHAIDFCQIDSCRL
                     GGLNEVIVVLLMAAKFGVPVCPHAGGVGLCEYVQHISLFDYICVSASLENRVLEYVDH
                     LHEHFVDPVVIRNGHYMPPQRPGYSIEMHAASLDQYDFPEGPAWRR"
BASE COUNT         2026 a         3459 c         3252 g         1928 t
ORIGIN      
        1 ctggatgatg ggtgcgtagt cctcgcgcgg acccttgggt tcgatcgtgt agccggccag
       61 cccctgccgg cgcatggcgg ccacatgaac gtccttttgc gccgccggca gccattcgac
      121 gatgcctatg gtctgaatgc cggagaagtt ggcgtcgaga ttgagcgtgc cgatgtagtt
      181 gcgaaattgg tcatggtcca cctcgccggt ggcgacgaac aggctctgcg cgccgcgcag
      241 cagcagttca tatgtcgcca tacgctgttc gacccggctg accgcgtcgc ccagcgaaaa
      301 gtcgaactgg gtgagcaatt catgacgggc ggcttgccgt tcgtggtccc aaagtatcca
      361 tgtaacactc agcgcggcgg acaagaccag taacggcacc aggacaaggc gcggccagct
      421 cacggtgggg tctggaagtc gaccatcgaa gcagcgaggt cgggtttgaa atacttctcg
      481 aagatccgcc ggacggtgcc gtccgcacgc atctcattca ccagggcgcg ccactttgcc
      541 cgctcggccg cggacagcgc ccgcttcgac atgatgaggc cgtgcggtac ggccgggtca
      601 ttgaactcga caatggtggt gacgtcgcgg attttctttt cgtcgagaac cggatagtcg
      661 aagggttcga tgatcatgcc ctggatgcgc cggaggatca gtgcctcata cagcgggccc
      721 aggccgcccg cctgggtgac gcgattttct tccgacagcg tgtcgaccag ccggttggcc
      781 gagtcgctat aacggaagct gcggatgacg ccgagctgaa actggtcgct gtgctcaaaa
      841 tcgctcagct gatgaatacc ggcatccttg cgcaccagta agtagtattt gtcgctgaaa
      901 taccaggcga aatccgcgta ctgaatgcgc tcgtcgttgg caatgccgga caggctgaaa
      961 tcgagcgcgc cggactcgat cagtttccag attcgcgcgc gcgacatgag gctgacccgg
     1021 atctggcagc cgctgcggcg gatcagttcg tcggcgaaat ccttgtcgat tccgctgtcg
     1081 gtgtcgactg aatagagcag cccgtgatcg tgcaatgcga gcgtaaatga acgcgaacaa
     1141 tcgggaccag ccgccattgc cttgccaaca aagcaaagcg caagcagcaa accgccaagg
     1201 ccatgtcgaa tcacaatttc tccatcgcga gaaggtggct tgaagataac cttccattaa
     1261 gccgacaacc gtctggacga ctgatttagc cggtgcgccc tgttccgttc cgttccgttc
     1321 cgtgtcgtga cagccggtca cgcaaggcga attttttgga agctgcctag cttacacctg
     1381 agcacatgaa atattgaaaa cattcaatcc acaaaagctc ctcacgtgtt atcggcggca
     1441 caccgcagtt cttgaggggc tgtggcgtta tttcatgcag ggcgttcgct catctgacgc
     1501 gccatctccg tttgcgaacg catccccctc gtgctccagc catgagcgga acagcacgac
     1561 cagatcatcc gcggacttct catccggcac ataagtgcaa tagcttcgtg acggcaaacg
     1621 aggaccggcg aacggcgcaa ccagccggcc cgcggcaaga tcgtccgcta ccagcgcggt
     1681 cggtcccatc gcaatgccga ttccgtcgat ggcagcttgc aacgtcagat agaagtggtc
     1741 gaaagacgct ggatcgcgtg cgtgatcgtc gcggccgggg cggtgtgtct ggggctttga
     1801 ccgtcaatcg gcaaaactcc agaagcaggc cggtacacgc cccctcgcgc gtggcttggc
     1861 agcacgcagt ccatctacga cgcgccaacc gctggccgta tcacgcaacg tgcccaggca
     1921 tctgcggaaa actaggcgcc cactttcagc aacaccggat cgttgcgata cagatcgggg
     1981 aacaggcgct ttaactcact caccttcggc agatcgttaa tcgcgatgta cggatagtca
     2041 gggtgaaggg cgaggaaatt ctggtggtag ttttccgccg gatagaaacc cttgaagtct
     2101 tctatccggg tcacgatcgg ccctgagaat acgtgcgcct tgtcgagctg cgcgatatac
     2161 gcttgcgcga cgctacgctg ctgggcattg gcgggaaaaa tcgccgagcg gtattgcgtg
     2221 ccgtgatcgg ggccctggta gttcaactcg gtggggtcat gcgcgaccga gaagaaaatc
     2281 tgcaataagc gcccgtaggt gatctgggtc ggatcgtaag tgatctgcac cgactccgcg
     2341 tgccccgtgt cgccttcgct gacggtttcg tattgcgcgg tgccggccgc gccgcccgtg
     2401 tagcccgagg caacctgctt cacgccccgc acgtgttcga ataccccctg aacaccccag
     2461 aaacagccgc ccgcgaatac ggcggtttcg ctatgcgccg taccgacctg ttcgtcctgg
     2521 gcaggcggcg gaaccttgac cgccgcttcc gctgaattcg ccatgcgctg cgcggcgaag
     2581 acgctcgccg caatggcgac gcatccggcg agcctgacca aggctgaacg tttgttccgc
     2641 gaaccctggc gggtggaagt cgtgtgcatg atgtcagtcc ttcagaaagt ggttgattag
     2701 cgccgcccgg cctgaatcaa ccgaacgtaa atgcataagc ttgcacgccg ggatcgagga
     2761 attcgatcga gaatgtatgg tccgcgacat cgccggtttg ccgaacgagt tgataaagac
     2821 gctgttcggt cacggtgccg ctaccgtcgg tggcgacgtc ggtaccgtgt gccgcgcctg
     2881 gtgctgcgcc atccacgctg acgcggaagc gaaccggctt gccgtccttg cccggaccca
     2941 gcacgagatg cagatcgcgt gcatgaaaac gatagacgat gcgtccggac ggtgccgcga
     3001 gcgtcgcgtg ttcagcgccc acgttccatg cgccggcgag gccccaatcg ttgacggcag
     3061 gctgcgacgg cgccgcgtag gtgtgcacct tgtcctgcgc ttcaccgccg ggcgacgcga
     3121 aattctccgc acgctcgtag ccgatgtagg tttccggtga ctgcatgtcg gcgttgtcag
     3181 ccgcggcctg cacaccttgc gcgctcgtgc cggcaatgcc gagcgcgacc ttagaggcgc
     3241 ctgcatggcc cgcttcagcc aacagttgct gaatcacctt ttccgattcc gcatagtcgc
     3301 cttcgccgaa atggtgataa cgaatctgcc ctttggcatc gacgaaatag tgcgccggcc
     3361 agtactgatt gttcagcgcg cgccagatcg cgtagttgtt gtcgatagcg acgggatagt
     3421 cgacgccgag gtcgtgcgtg gccttcttga cgttgtcgat attccgctcg aacgcgaatt
     3481 ccggcgcatg cacgccgatc acgaccaggc cctgatcctt gtacttctgc gcccacgctt
     3541 tgacatacgg cagcgaacgc aggcagttga tgcacgaata ggtccagaaa tcgacgagca
     3601 cgaccttgcc gcgcagaccc tgaaccgtca gcggcggtga attcagccat tggactgcac
     3661 cgtccagcgt cggcagcacg ccttcgatgg gcaacggggc cggctcgccg gctggtgcgc
     3721 cttgcgcggt gcgcatcatc gcgccggaac cggcggaggc gcttgtgtcg gcagcggggt
     3781 tggcggtcat cacggcgtcg ctggctgatg cgctggccaa tgcattagcc gacgcgttgg
     3841 cgctgctccc cgccgccacc ggattgacag gtgtcgcgcc gggcgaaagc ttatcgacca
     3901 gtttctgttc gatgccgccc gtggcgacgg tcgagatgcg cgtcagcacg cctgtatcga
     3961 ggcccaacgc aatcgccacc acgccgcaca acatcgccgc gccaattccg cggcggatcc
     4021 attcgcccgc gcccagcgag cgcttcatcg ccgtaaacac ccggccgccg atcagcaagg
     4081 cgaccgcgag cgaagtcgcg gccccggccg catacgccac cagcaacagc gtggtgccga
     4141 cgctcgcccc ccgcagtgcc gcgccagtca gcaccagacc gagaatcgga ccggcgcacg
     4201 gcgcccacag caagccggta gcgataccca gcagaaacga cgaacctggg cggccctgct
     4261 ggccgtctgt ctgggcaaag ttcgacagcc ggttacccgc gctcaccagc gggcgcatca
     4321 gatgatccgc aaagcgcgga aacagcagcg acagtccgaa cacggcgagc aacgcgattg
     4381 cgagccagcg gccatactgg ttggcctgag tggcccagcc gccgcccacc gcagcaagcg
     4441 tcgcgacgac ggcgaaggtc agcgccatgc ccgcaagcag cggcagcccg ctgcgcacga
     4501 atggctgatc agcacgcgcg aaaacaaacg gcaacaccgg caggatgcac ggactaagaa
     4561 tcgtcagggc gccgccgaga taggcaagaa cgatcagtag catgtcggtt ccagacgcag
     4621 gaatctgcgg aaagtggcca catcctaagg cgcgggggat accgaagtcc tcacgaaaag
     4681 ttaaattaaa tgtgataact cgccgcccga aaattttgca caatgacgca acggcccgct
     4741 tttgcgggcg cccttgtttt gcagtgccaa accacgccct cgccggcgtc tgaagccctc
     4801 catggaccac ccgaagcgca tcctgattgt cgaagacgac gtcgatatcg ccaacctgct
     4861 gagccttcat ctgcgcgacg agcgttacga agtcgtgcac agcgccgacg gcaacgaagg
     4921 gctgcgtctg ctggagcaag gcaactggga cgcgctgatc ctcgatctga tgctgcccgg
     4981 cgtcgacggt ctggaaatct gccggcgcgc ccgcgcgatg acgcgctata cgccgatcat
     5041 catcaccagc gcgcgttcta gcgaagtgca ccggattctc ggcctcgaac tcggcgccga
     5101 cgactacctc gccaagccgt tctcggtcct cgaactggtg gcgcgcgtca aggcgttgct
     5161 gcggcgcgtc gaagccatgg cgaaagactc gcgaatcgac gcgggcagtc tgcggattgc
     5221 cggcctgacg atcgatccgc tgacgcgcga ggcgagcgtc gacggcaaag ccatcgaatt
     5281 gacgccgcgt gaattcgacc tgctctacta cttcgcgcag catcccggca aagtgttctc
     5341 gcgcatggac ctgctcaatg ccgtgtgggg ttatcagcac gagggctacg aacataccgt
     5401 caacacccat atcaaccggc tgcgcgccaa gatcgaggcc gatccggccc agcccgagcg
     5461 catcctgacg gtctgggggc gcggctacaa actggccgtg ccgggtattc caggcgacgg
     5521 gccggcgtcg aacagcgcac ctcgggacac gccgtgactc tgacactcac ccaacggctc
     5581 tcactcgttt tctccgcgct gctgctggcg tgctgcggcg cgtcggcgtg gctgcagatc
     5641 cgctcgagcg acctgcacga aaaggaagtc gtgcaaagcc tgtcgcgcag cctcgccgac
     5701 catatcgcgc accgcacgac cctgatggat gcgaatggtc tgcgaccgga tgccgtgcgc
     5761 caattattcg gccagttgat gatggtgaat ccgagcgtcg aggtgtacct gctcgacaat
     5821 caggggcgca tccagggcga cgacgctcct gccggtcacg tcaagcgcca gcaggtggat
     5881 ctcgcgccga tccggcaatt catcgccggc gatgcgttgc cgattctcgg cgacgacccg
     5941 cgcagcctgg acggcaaaaa ggtgttcagc gccgcaccgc tgcaactgag cggccggcaa
     6001 ccctccggct acatctatgt cgtcctgctc ggcgaggcgc acgacgcgct cgccgcacgc
     6061 gtggccgcca gctcggtgct gcgcaccacg ctgtggtcga tggcgctggt cgcgctgctc
     6121 ggtttgctgg cgggcctgac ggccttcggc ctcatcacgc ggccgctgcg ccgcctcacc
     6181 gacgccatgc gccgcttcga cgccgacggc gagccggaca cccagccggc cgtgccgcgc
     6241 tcggcaccgg ccggacagcg cgacgaaatc gccgtgctcg aaaccacgtt cgcacaaatg
     6301 gcgaaccgca tcggcgaaca gtggcgcgcg ctgacgagac aggatcagca acggcgcgag
     6361 ctgatcgcca atatttcgca cgacctgcgc acaccgctca cgtcgctgca cggttatctg
     6421 gagacgctgt cgctcaaggc cgacacgctc ggcgaaaccg agcgcaaacg ctatctggcg
     6481 attgcgctgg cacagagcgt caaggtgggg cggctcgcgc agtcgctatt cgagctggcc
     6541 cggctcgaac acggcaacgt gcaacccgcg ctcgagcagt tctcgctggt cgatctggtg
     6601 caggatattt ttcagaaatt cgagttaccg gccgaggcgc ggcacatcca gctgcgggcg
     6661 gagattcccc cgcgcctgcc ggccgtcagc gccgatctgg ggatgatcga gcgcgtgctg
     6721 accaatctgc tcgataacgc gattcgccac acccccgccg gcggcgtgat cgacgtcgaa
     6781 ctcgcgcaca aggacggcaa ggtctcggtc acggtgagcg acactggaac cggcattcca
     6841 gcggaacagc gcgaggggct gttcgaacgc cccttcagct cagccggcgc gcatcgcggc
     6901 ggcggcctcg gcttgctgat cgtgcagcgc atgctgcaat tgcatcagag ccagatccgc
     6961 ctgatcgaac gtgcgggccc cggcacgacg ttctgtttcg atctgccgac aaccgggaaa
     7021 tcgactgccg ccggcatccc gctgtgactt gatctcacgg ccgcgcgccg cagccgccaa
     7081 ttcctcgccg tagcagcgtg cttcgccgcg ctttccattc attctctcca gttatatccg
     7141 cctgaatata acgagcgagt ggttccatat ctacgtcgcg cgccagctta ctgtgtgtca
     7201 taaagacgac aacttcgctt cgggacgcaa cggtacgcac gacgtgcaag cccagatcgg
     7261 ttcgagcacg agcttcttcg ggccgtccgg ttcgggcgcg gacaaccagt tggcattccg
     7321 ggtcggcgtg cgccaccggt tctgacaccc aggtctctcg cgagcgacag cgaggctcag
     7381 tacccaggca atgggccgag ccaggactaa aaaatgccgc ccgtgttcac gcgggcggca
     7441 tttttttcgc cgggattaaa gaccgagccg gttttcacgc atctttcagc cagggcattt
     7501 ccaccggcga caccaccaga tgccgggact cccgtttcag ttcgaagatg aagccgacga
     7561 ggatcatcga gtcggcatac gggattggga atacgtgagg attttccgcg gcgcgcagtg
     7621 ggtgagccgg cgtatccgcc gtcgtcgcgg cgttcgcgcg gacgctttcc agttcgtcgg
     7681 ccatggcctg gccactgact ttcaggacgc ggctacgcaa atcgctaaac ggttcgatgc
     7741 gcgccccgtc ttcgttgaat tcgggcactt cgtagatgaa ccaggacatt tgcgtctccc
     7801 aagaaaaggg aagctatttt agcagtgggg ctaatgggct atcacgtatg cacatcctgc
     7861 tgcggctccc ccgttcattc gccgtccaat gcaggcaatg caatcgcata cacggtgacg
     7921 cacgtgtcgc tgccgccctg ttccagacga ggcccaccac atcgatccgc ggcctcacac
     7981 tggcgctccg tgccgaagcg attcccccgg aatcgttaca atggtccaga tgattcgccc
     8041 ctcaacagca tcaacagcaa accgcatcgc gtgcaagaca tcctgacctg ctatttccag
     8101 agtggcgacg ccatccgcag tcgacgcgtg agcgacatcg tgctatcagg cacgctcgac
     8161 gtgcctgcgc cctccgcacg tctgcttgcg gattgggaaa gggagacgtc gttgcacatg
     8221 gtcctcgagc ccggtgacgt cgaagtcatg cctttggcgc gagcgcgggt gcgctggccg
     8281 gactacacgc gctgcgtgca ggccatgtcc gattggacgt gtgcgctcgg actgcccgag
     8341 gtgcttgccg ccagtgacgt agccctcatg gcctgccgcg gcgcgaggta ccaccatgac
     8401 ggtgcgcaat atggcgattc ggccttttgt aatctcttca tgagcgagga caagggtctc
     8461 gacttgcatt ttccctctac gggccagcgc attccgctga cccggggaac ggccgtgata
     8521 ttcgataccg gccagcctca tggcgtagtt cagcgcggta gcagcggttt caatgcagcc
     8581 gagttcacgt ccgctcagga ttgcgtccag atattcctga cctgggagct gccgatcgaa
     8641 gatccccatg tcgggcaggc actcaacgtt gccttcgaca tcgccccggc ggcctcgtcg
     8701 caaccggatg aagagcaagt cttgctgaac ggcgaacggg tcattgtgtg cccggattct
     8761 ggccgttggc gcccggtcga ctaaacgtcc gcgccgcacg gtcatgttgc cctgacaggg
     8821 cggacaagct cacgttcaac gcggtacacg catgcagcaa gcgctattgc accgggatcc
     8881 gcgacgatgc cggacgtcat tcgagaccgg atgcccaagg ccaccgcttc gcgccgccag
     8941 cccgcctgta tgatggcggg ctggcgccgg accgaaagcc cgcgcaggcg gcccgcgcag
     9001 atcgcgcatc gcctgccgcc cgctgccgcc tcgcggcgcc aatgcatcca ttcacgatgt
     9061 tcacgatgac ccaaaggaag accgccatga cggccgcaac gcaattcgat caggtttccg
     9121 ttatcaaacg cgccaacgtt tacttcgacg gcaagtgtgt ctcgcacacc gttctcttcg
     9181 ccgacggcac acgcaagacg ctcggcgtga tcctgcccgg cacactcaac ttcggcaccg
     9241 acgcgccgga actcatggag gtgcaggcgg gccaatgccg tatccgcctc gaaggcagtg
     9301 acgaatggaa gacgtatggc gcgggcgagt cgttctcggt gcccggcaag agccgcttcg
     9361 atatcgacgt gaccgaaacg ctcgactacg tctgcagcta tctgtgaaga aagacgggcg
     9421 catggcgccc gtcgtgcgcc tgaccggacg cgcctgctag tggcgcgtcc tgatcgcccc
     9481 ctgcgcacgg gcacctgaaa caacgccccg gcgcgtgcga tctcagcgcc gccaggccgg
     9541 cccctcagga aagtcatact gatcgagcga cgccgcgtgc atttcgatgc tgtagccggg
     9601 acgttgcggc ggcatgtaat gaccgttgcg gatcaccaca ggatcgacga aatgctcatg
     9661 cagatgatcc acgtattcga gcacccggtt ttccagcgac gccgacacgc agatatagtc
     9721 gaacagtgaa atgtgctgga cgtactcgca caaaccgacg ccgcccgcgt gcggacacac
     9781 gggcacgccg aacttggccg ccatcagcag gaccacgatc acctcgttca gaccgcccag
     9841 ccggcagcta tcgatctgac agaagtcgat ggcatgcgcc tgcagcagct gcttgaacat
     9901 cacgcggttc tgacagtgct cgcccgtcgc cacgccgatt gggctgattc gctggcggat
     9961 ggccgcgtgg ccgagaatgt cgtcgggact cgtgggttcc tcgatccacc acggatcgaa
    10021 ttccgccaga cgccgcatgt tagcgaccgc ttcatcgacg tcccacacct ggttcgcatc
    10081 catcatcagc ttccggtttt cgccgatttc ctcgcgcagg atgcgggccc ggcgcatatc
    10141 ttcctcgaga ttgccgccca ctttctgctt gaagtgggtc cagccctgcg cgacgccttc
    10201 gcgtgcgagc cggcggatct tgtcgtcgtc gtagccgagc cagccggccg acgtcgtgta
    10261 ggccggatag ccgtgcgcga gcatttctgt ctcgcgctcg ccgcgggtcc cggcatgccg
    10321 atgcagcatg gcgatggctt cctgcggcgt gatcgcatcg gtcacatagc ggaagtcgag
    10381 acagcgcacg agtgcttcgg ggctcatatc gacgagcagc gtccacaccg gcttgccttc
    10441 cgcttttgcc cacaggtccc aggccgcatt caccacagcc gccgtcgcca gatgaatggc
    10501 gcccttgtcc gggccgatcc agcgcaactg gctgtccgag gtcagcgcgc gccagaaagc
    10561 gcccatgttc gcggcgatat cctcaagcgt tgtgccgacg acgagcggcg ttagcgcctc
    10621 caccgccgtc acgcagatct cgttgccgcg cccgattgtg aacgt
//
LOCUS       DATASH010000143        10060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4569459,
            whole genome shotgun sequence.
ACCESSION   DATASH010000143 DATASH010000000
VERSION     DATASH010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4569459"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VHV99_04305"
     CDS             <1..216
                     /locus_tag="VHV99_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaJ"
                     /protein_id="HEX3634699.1"
                     /translation="GIAPPMGNCGSNEHAPQERPIRFVPRVVDKPACPRCGSSHTERL
                     AQFGSTACKALYRCMDCREPFDYFKPY"
     gene            225..1313
                     /gene="paaE"
                     /locus_tag="VHV99_04310"
     CDS             225..1313
                     /gene="paaE"
                     /locus_tag="VHV99_04310"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019255661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HEX3634700.1"
                     /translation="MATPQFHSLRIRDVRPETADAVTVSFDVPENLRDAFRFTQGQFV
                     TLKTHIDGEETRRSYSICVGVTDYDRDGELRIGIKRVRGGRFSNFAFDTLQPGHTIEV
                     MTPDGRFFTHLNADQGQQYIAFSGGSGITPVLAIIKTTLEVEPRSTFTLVYGNRSVDQ
                     IMFAEELEDLKNRFMNRFVLYHVLSDDLQDVELFNGVLDQQKCAAFIENLLPADAIDE
                     AFICGPGPMMDAAEAALKAAGVPAEKVHVERFGSPLPQAGVPPVEITDDTPAADLEIV
                     LDGKRRKLRLPYQGVSVLDVGLRAGLALPYACKGGVCCTCRAKVLEGEVKMEKNYTLE
                     EHEIRDGFVLTCQCHPISDRVVVSYDER"
     gene            1480..2292
                     /locus_tag="VHV99_04315"
     CDS             1480..2292
                     /locus_tag="VHV99_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1835 domain-containing protein"
                     /protein_id="HEX3634701.1"
                     /translation="MSTIHLTNGDVAAESLRAALAEAGRDDRVHALRDDLAVGPLRGI
                     DDGPDVRAEFWDRVSTDTRRDFLREFREQAAALENIARGDANLVVWHGESAADQLTLR
                     RVCYHLRNSPQRLNEVRLSIRDLTDPGAWAHSRKDRATSVGMFAPAVLQGRLSDAAPI
                     SVLRISRLALEWQEAKHANGETRRWRDNTFTSGSFADLDALILEHVTGDWQPAARVAA
                     ELMIAELWYLVSDSIVLWRFRELAATRRIKLRGDASAWRTLQLCAALAPCPH"
     gene            2307..2915
                     /locus_tag="VHV99_04320"
     CDS             2307..2915
                     /locus_tag="VHV99_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX3634702.1"
                     /translation="MARTRAPDHETQRDQILELAAAKFAQTSYPSTSMADLAAASGTS
                     KARLYHYYASKEAILFDLLDRYTKRLMLIIAEVEGASQRRGLSERETFAELIRAFLSE
                     YETSHSRHVALLNDVKYLVDSQREVILNRQRDVVAAFARQLARAYPERATRENQTALT
                     MMVFGMINWTFTWLKPDGRMGYREFAEQVVNMVDHGLSAAEV"
     gene            3156..3824
                     /locus_tag="VHV99_04325"
     CDS             3156..3824
                     /locus_tag="VHV99_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3634703.1"
                     /translation="MNLITPRAAEPIVAHGRLPQPAGRAPALVRELTAVDRERLLAHF
                     LSLDEDDRLLRFGQIVPNHVIENYVRAIDFTRDTVFGVFNSQLQLTGVGHLAYLPAEG
                     DKRTAEFGVSVLESVRGQGIGSKLFERAAIRSRNTHVTTLYMHCLSRNSTMMHIAKKA
                     GMKIEYAYGEADAYLTLPPADQSSIIAEMLQEQAAVFDYALKRQARHASKLFESFLPA
                     AEAA"
     gene            complement(3909..4406)
                     /locus_tag="VHV99_04330"
     CDS             complement(3909..4406)
                     /locus_tag="VHV99_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX3634704.1"
                     /translation="MANIEIDAIDRRILAILQENGRLSNQEIAERINLSPSPCLRRIR
                     RLEESGVIRGYVALLDAQKLGLDLLAYVNVRLEKRSGPALSPRGDATHADLFRAAVQT
                     WPEVVACHAMTGDMDYLLRVQVEDMAHFSRFVQDQLLHHPSVIDVKTSFSLEKFKETT
                     ALPIL"
     gene            4570..5667
                     /gene="hppD"
                     /locus_tag="VHV99_04335"
     CDS             4570..5667
                     /gene="hppD"
                     /locus_tag="VHV99_04335"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006497422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEX3634705.1"
                     /translation="MQVSTWENPVGTDGFEFIEYTAPDPKALGKLFEQMGFTAVARHR
                     HKDVTLYRQGEINFIVNGEQDSFAQRFTRLHGPSICAIAFRVQDAAKAYRQALEKGAW
                     GFDNKTGPMELNIPAIKGIGDSLIYFVDRWRGKNGAEPNSIGNIDIYDVDFEPIPGAN
                     PNPVGHGLTYIDHLTHNVHRGRMQEWAEFYERLFNFREVRYFDIEGKVTGVKSKAMTS
                     PCGKIRIPINEEGSDVAGQIQEYLDEYHGEGIQHIALGSNDIYRTVDGLRAANISLLD
                     TIDTYYELVDRRVPNHGEPLDELRKRKILIDGAPDDLLLQIFTENQIGPIFFEIIQRK
                     GNQGFGEGNFKALFESIELDQIRRGVVQDKA"
     gene            complement(5805..6005)
                     /locus_tag="VHV99_04340"
     CDS             complement(5805..6005)
                     /locus_tag="VHV99_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006053047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634706.1"
                     /translation="MNTRNIQTFLMVSAAFFAMSAPVRSNSNSAGTLLNAITSTAQVA
                     PARIAVERVARSTLAGEEDQNS"
     gene            6341..9940
                     /locus_tag="VHV99_04345"
     CDS             6341..9940
                     /locus_tag="VHV99_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEX3634707.1"
                     /translation="MNAPLDPGQRASLEAALSSVTLDDKYTLERGRAYMSGIQALVRL
                     PMLQQQRDQAAGLNTAGFISGYRGSPLGGLDQSLWKAKQHLAAHRVVFQPGVNEDLAA
                     TAVWGSQQVNLYPSAKYDGVFSMWYGKGPGVDRSGDVFKHGNSAGSAQHGGVLVLAGD
                     DHAAKSSTLAHQSEHIFKACGLPVLFPSNVQEYLDFGLHGWAMSRYSGLWVAMKCVTD
                     VVESSASVDIDPHRTKIILPDDFAMPEGGLNIRWPDPPLVQEARLLDYKWYAALAYVR
                     ANKLDRVEIDSPHARFGIMTGGKAYLDVRQALTDLGLDDETCSRIGIRLYKVGCVWPL
                     EAQGAQAFARGLEEILVVEEKRQILEYAIKEELYNWPDAQRPRVFGKFDEKDGAGGEW
                     SVPMGNWLLPAHYELSPAIIAKAIATRLDKLDLPSDVRARIATRIAVIEAKEKALARP
                     RVEAERKPWFCSGCPHNTSTNVPEGSRAMAGIGCHYMTVWMDRSTSTFSQMGGEGVAW
                     IGQAPFSNDKHVFANLGDGTYFHSGLLAIRAAIASKANITYKILYNDAVAMTGGQPVD
                     GVLTVPQITHQLAAEGATKIAIVTDEPEKYAANVGLAPGIDIHHRDKLDEVQRQLREI
                     PGTTILIYDQTCATEKRRRRKRGTYPDPARRVVINEAVCEGCGDCSVKSNCLSVEPLD
                     TEYGTKRQINQSTCNKDFSCVNGFCPSFVSVEGGQLRKPKAASGIVGDAMPPVPEPEL
                     PSIARPYGVLVTGVGGTGVVTIGALLGMAAHLENKGVTVLDVTGLAQKGGAVMSHVQI
                     AQNPADIHATRIAMGEASLVIGCDAIVTASDECVSRMQVGRTRVVLNSAHTPTAELIK
                     NPNWRFPGSSTEADVRAGAGDDGVDMVDANHFAVALLGDAIYTNPFVLGYAWQRGWVP
                     LTYQSLMRAIELNNVQIEKNRAAFEWGRRAAHDLAAVRKLAQSQGRGAAAETASSKII
                     SLHTPKALDTLIDKRADYLAAWQNDAYARRYRDLVSQVRVAESALDSIDGQLPLTEAV
                     AKNLHKLMAYKDEYEVARLYSDPAFIEKLKANFEGDWKLHIHLAPPTFSKKDAQGHLV
                     KKKYGPWVFSAMHVLAKLKFLRGTAFDVFGKTEERRTERALIVEYEALVRELIGGLTA
                     QKRELAIELANLPDGIRGYGHVKENNLKGVRVKWNGLLTRWRSPETGKTQHAA"
BASE COUNT         2083 a         3109 c         3017 g         1851 t
ORIGIN      
        1 ggcatcgccc cgccaatggg caattgcggt tcaaacgaac acgcgccgca agaacggccg
       61 atccgcttcg tgcctcgcgt ggtggacaaa ccggcctgcc cgcgctgcgg atcatcgcat
      121 accgaacgcc tcgcccaatt cggctcgacg gcgtgcaagg cgctgtatcg ctgcatggat
      181 tgccgcgagc cgttcgacta cttcaaaccg tactgaaaac gaccatggcg actccgcaat
      241 tccattcgct gcgcatccgc gacgtccgtc ccgaaaccgc cgacgccgtc acggtatcct
      301 tcgacgtccc cgaaaatctt cgggatgcat tccgcttcac gcaaggccag ttcgtcacgc
      361 tgaagacgca catcgacggt gaggaaactc gccgttcgta ttcgatctgc gtcggtgtca
      421 ccgactatga ccgcgacggc gagctgcgca tcggcatcaa gcgcgtgcgc ggcggccgct
      481 tctcgaactt cgcattcgac acgctgcaac ccggccacac catcgaggtg atgacgccgg
      541 acggccgctt cttcacccat ctgaacgccg accagggcca gcagtacatc gcgttttcgg
      601 gtggttcggg catcacgccg gtgctcgcga tcatcaagac cacgctggaa gtcgagccgc
      661 gcagcacctt cacactggtg tacggcaacc gcagcgtcga tcagatcatg ttcgcggaag
      721 agctcgagga tctgaagaac cgcttcatga accgcttcgt gctttatcac gtgctatccg
      781 acgatctgca ggacgtcgag ctgttcaacg gtgtgctcga ccagcagaag tgcgcggcct
      841 tcatcgagaa cctgctgccg gccgatgcaa tcgacgaagc cttcatctgc ggccccggcc
      901 cgatgatgga tgccgccgag gctgcgttga aagccgcggg cgtgcctgcg gaaaaagtgc
      961 acgtggagcg tttcggttcg ccgctgccgc aagcgggcgt gccgccggtc gaaatcaccg
     1021 acgacacccc cgccgccgac ctcgagatcg tcctcgacgg caaacggcgc aagctgcgtc
     1081 tgccgtatca gggtgtgagc gtgctcgacg tcggcttgcg cgccggcctc gcgctgccct
     1141 atgcatgcaa gggcggcgtc tgctgcacct gccgcgcaaa ggtgctggaa ggcgaagtga
     1201 aaatggaaaa gaactacacc ctggaagaac acgaaatccg cgacggtttt gtgctgacct
     1261 gtcaatgcca tccgatcagc gaccgcgtgg tggtgagtta cgacgagcgc tgagtcaagc
     1321 gcggcagggg accctgcgac gcccgcgacg ctttcagcag gtccgcgatc cgcttacact
     1381 aggggccgct cgcgatcgga catctgtccc gatcgatgag cggccccttt tgtcatttgc
     1441 gttcttattt ccgttttttg ccagcccaag ccactgccga tgagcacgat ccacctcacc
     1501 aacggcgacg tcgccgccga gtcactacgc gccgcgctag ccgaggcggg ccgcgacgat
     1561 cgcgtgcacg cactgcgcga tgacctggcg gtcggtccgc tgcgcggtat cgatgacgga
     1621 cccgacgtgc gggcggaatt ctgggaccgc gtcagcactg acaccaggcg cgattttctt
     1681 cgggagtttc gcgaacaggc ggcagcgctg gagaacatcg cgcgcggtga cgcgaacctg
     1741 gtggtctggc acggtgaaag cgcagccgat caacttacgc tgcgccgcgt ctgctaccac
     1801 ctgcgcaaca gcccgcaaag gctgaatgaa gtgcgtctgt cgattcgcga cctcaccgat
     1861 ccgggtgcat gggcgcatag ccgcaaagat cgtgcgacct ccgtcggcat gttcgcgccg
     1921 gctgtactgc aagggcgttt gtcagatgcg gcgccgattt cagttctgcg catcagccgg
     1981 ctcgcgctcg aatggcagga agccaaacac gcaaacggcg agacacggcg ctggcgcgac
     2041 aacaccttca cgagcggcag cttcgccgat ctggacgcgc tgattctcga gcacgtcacc
     2101 ggcgactggc aacccgccgc gcgtgttgcc gccgaactga tgatcgccga actctggtat
     2161 ctggtaagcg acagcatcgt gctgtggcgc ttccgcgaac tggccgcgac gcgccgtatc
     2221 aagctgcgtg gcgacgcgag cgcgtggcgc acgctgcaac tgtgcgcggc gcttgccccc
     2281 tgtccacact aataaaatag acaactatgg cccgcacccg agcccccgac cacgaaacac
     2341 agcgcgatca gattctcgaa ctagcggcgg cgaaattcgc gcagaccagc tacccgagca
     2401 cctcgatggc cgacctcgcc gccgcgagcg gcacctcgaa agcgcgcctg taccactact
     2461 acgccagcaa ggaagcgatc ctgttcgatc tgctggaccg ctacaccaag cggctgatgc
     2521 tgatcatcgc cgaggtggag ggcgcgagcc aacgtcgcgg actgtcggag cgggaaacct
     2581 tcgcggagct gatccgcgcg tttctgtccg agtacgagac ctcgcatagc cggcacgtcg
     2641 ctctgctgaa cgacgtgaag tatctggtcg acagccagcg ggaagttatc ctgaaccgcc
     2701 agcgcgacgt ggtcgcggcg tttgcgcggc agttggcgcg cgcctatccg gaacgcgcca
     2761 cgcgcgaaaa ccagaccgcc ctcacgatga tggtgttcgg catgatcaat tggacattca
     2821 cctggctcaa gccggacggc cgcatgggct accgggaatt tgcggagcaa gtggtcaata
     2881 tggtggacca cggactcagc gctgccgaag tctgattgcg catagcagca tcaccaaaaa
     2941 gggcaatcgc cacgggttgc ctagacgcga caagttgtcc cgaacgctcg ttcaatttat
     3001 ttatctttaa taatcaataa cttataaata ggtctccagc cttttaggca ttcacctcag
     3061 ttttgatacg ggttttccct agtgatgagc cagggtttcc gctaaaatcg tttttgcggt
     3121 gcagcacgcc gctcgattaa aaggagaacc tgaccatgaa cttgattaca ccccgcgccg
     3181 ctgagccgat cgtcgcgcac ggtcgcctgc cgcagccagc cggtcgtgca cctgctctgg
     3241 tccgcgaact taccgccgtc gatcgtgaac gactgctggc ccacttcctt tcgctcgacg
     3301 aggacgatcg ccttctacgc ttcggccaga tcgtgccgaa ccacgtcatt gaaaactacg
     3361 tccgcgcgat cgatttcacg cgcgacacgg ttttcggcgt gttcaatagt cagctgcaac
     3421 tcactggcgt cggtcatctc gcgtatttgc ctgccgaagg cgacaagcgc accgccgagt
     3481 tcggcgtgtc ggtgctggaa agcgtccgcg ggcaaggcat cggctcgaag ctcttcgaac
     3541 gcgccgccat ccgcagccgc aatacccatg tcaccacgct ttacatgcac tgcctgtcgc
     3601 gtaattcgac catgatgcat atcgcgaaga aggccggcat gaagatcgaa tacgcgtatg
     3661 gcgaagcaga cgcctatctg acgctgccgc cggcggatca atcgagcatc atcgctgaaa
     3721 tgctgcagga acaggccgct gtgttcgact acgcactcaa gcgccaggcg cgtcacgcgt
     3781 cgaaattgtt cgaatcgttc ttgccggcag ccgaagccgc ttaaccggtc tgcgcgtcgg
     3841 cgaaaaaagg gcggccttgt ggctgccctt tttgtttgta tatcgcaccg cccgctgccg
     3901 ccccgcgctc acaaaatcgg cagcgccgtc gtctccttga atttctcgag cgaaaagctc
     3961 gtcttcacgt cgatgacaga tggatggtgc agcagctggt cctgcacgaa acgtgaaaag
     4021 tgcgccatat cctcgacctg cacgcgcaac aggtagtcca tgtcaccggt catcgcgtga
     4081 catgcgacca cttccggcca cgtctgcacc gctgcgcgga acagatcggc atgcgtggcg
     4141 tcgccgcgcg gactgagcgc cggaccgctg cgcttttcca gccgcacatt gacgtaggcc
     4201 agcagatcga gcccgagctt ttgcgcatcg agcagcgcga catagccgcg aatcaccccg
     4261 ctttcttcca gccgacggat acgccgcagg cacggactcg gcgacagatt gatgcgctcg
     4321 gcgatctcct gattcgacag cctgccattc tcctgaagaa ttgccaggat gcgccggtcg
     4381 atagcatcta tctctatatt ggccattttc tgccgtccaa gtaaaaaatt gagctagaaa
     4441 atagcgccag cgtagcggcg gaagattaag ttcgcaaggt ttcgtcacta accggccttt
     4501 acacttgcgt cattcagttc agccgcgctc aatcgcacac cccgcgccag cgcttagagg
     4561 agacagacaa tgcaggtttc aacttgggaa aacccggtcg gcactgacgg cttcgagttc
     4621 atcgaataca ccgcgccgga cccgaaagca ctcggcaagc tgttcgaaca gatgggcttc
     4681 accgccgtag cccggcatcg tcataaagac gtgacgctgt atcgccaggg cgaaatcaac
     4741 ttcatcgtca atggggaaca ggattcgttc gcgcaacgtt tcacgcgcct tcatggcccg
     4801 tcgatctgcg cgatcgcatt ccgtgtgcaa gacgcggcga aggcttaccg gcaggctttg
     4861 gaaaaaggcg cctggggctt cgacaacaag accggcccga tggaactgaa catcccggca
     4921 atcaagggca tcggcgactc gctgatctat ttcgtcgacc gttggcgcgg caagaacggc
     4981 gctgagccga acagcattgg caacatcgat atttacgacg tcgacttcga accgattccc
     5041 ggcgcgaatc cgaatccggt cggccacggc ctgacctaca tcgatcacct cacacacaac
     5101 gtccatcgcg ggcgcatgca ggaatgggcg gagttctacg agcgtctgtt caacttccgg
     5161 gaagtacgtt acttcgacat cgaaggcaag gtgaccggcg tcaaatcgaa ggcgatgacc
     5221 tcgccgtgcg gaaagatccg cattccgatc aatgaggaag gttccgacgt ggctggtcag
     5281 attcaggaat atctggacga gtaccacggc gaaggcatcc agcacatcgc gctgggcagc
     5341 aacgacatct accgcaccgt ggacggtttg cgcgcggcga acatttcgct gctcgatacg
     5401 atcgacacgt actatgaact ggtcgatcgc cgcgtgccga accacggcga gccgctcgac
     5461 gaattgcgta agcgcaagat cctgatcgac ggcgcacccg acgacctgct gctgcagatc
     5521 ttcaccgaaa accagatcgg gccgatcttc ttcgagatca tccagcgaaa aggcaatcag
     5581 ggcttcggcg agggcaattt caaggcactg ttcgaatcga tcgagctgga ccagattcgc
     5641 cgtggcgtgg ttcaggacaa ggcctgaagg ctgaaggccg gcgcggaatc tgagagatat
     5701 aaaaaaggcg aggatcgcaa gatcctcgcc cttcgtcgtt ggtgccgttc tccgacaccc
     5761 ggacaacggc caattttcaa ccgacgcgcc gcatcagcaa cgcatcatga attctggtct
     5821 tcctcgccgg ccaaagtgga ccgagcaacg cgctccaccg caatacgtgc gggcgcgact
     5881 tgcgccgtgc tggtgatcgc attcaacagc gtccccgcgc tgttcgaatt cgagcgcacc
     5941 ggcgcgctca tggcgaagaa tgcggccgaa accatcagga aggtctggat gtttctggtg
     6001 ttcatgcgta ctccttttct aactgtcctg ctgtcctgaa gccccgccga atgcggagcg
     6061 ttgcgcagac attgctcgca ggtgggctgt tagacagaca tatcaactgc ggttccgcag
     6121 atttgtagtt tttacagctt gttacagtgg cattgcggcg ctggacgtaa ttcggtgaag
     6181 tttcatgctg ggacatcccc ctgcgggctg cggcgggttt ttaccagtgc taccatcact
     6241 taaaacacgt caatatggcg accccggccc caagcattca caagatgccg cggcccaaaa
     6301 aagttttggt gatcccaaac tggttggagg agtacacata atgaatgccc cgctagaccc
     6361 aggtcagcga gcctcgctcg aagccgcgct atcttccgtc acgctcgacg acaaatacac
     6421 cctcgaacgc ggccgcgcgt atatgagcgg catccaggcg ctggtgcgtc tgccgatgct
     6481 gcagcagcaa cgcgatcagg ccgccgggct caataccgcc ggttttattt ccggttaccg
     6541 tggatcgccg cttggcggac tcgaccaatc cttgtggaag gcgaaacagc atctcgccgc
     6601 acatcgcgtc gtgttccaac ccggcgtcaa cgaagacctc gcggcgaccg ccgtgtgggg
     6661 ttcacagcag gtcaatctgt acccgagcgc caaatacgat ggcgtgttct cgatgtggta
     6721 cggcaagggc ccgggcgtcg accgctccgg cgacgtcttc aagcacggca attcggctgg
     6781 ctcggcacaa cacgggggcg ttctagtgct tgccggcgac gaccacgccg ccaaatcctc
     6841 gacgctcgcg caccagtcgg aacacatctt caaggcgtgc ggcctgccgg tgctgtttcc
     6901 atcgaacgtg caggaatatc tggacttcgg cctgcacggc tgggcgatga gccgctattc
     6961 cggcctgtgg gtggccatga agtgtgtgac cgacgtggtc gaatcgtcgg cttcggtcga
     7021 tatcgatccg catcgcacga aaatcattct gcccgacgac ttcgcgatgc ccgaaggcgg
     7081 cctgaacatt cgctggccgg atccgccgct ggtgcaggaa gcgcgtctgc tcgactacaa
     7141 gtggtacgcg gcgctagcct atgtacgcgc gaacaaactg gaccgcgtcg aaatcgattc
     7201 accgcatgcg cgctttggca ttatgaccgg cggcaaggcg tatctcgatg tgcgtcaggc
     7261 actcaccgat ctcggtctcg acgacgaaac ctgttcgcgc atcggcatcc gtctgtataa
     7321 agtgggctgt gtatggccgc tcgaggcgca aggtgcgcaa gcgttcgcgc gcggtctcga
     7381 ggaaattctg gtggttgaag aaaaacgcca gatcctcgaa tacgcgatca aggaagaact
     7441 gtacaactgg cccgatgcgc agcgtccgcg cgtgttcggc aagttcgatg aaaaagacgg
     7501 tgcaggcggc gaatggtcgg tgccgatggg caactggctg ctgccggcgc actatgagct
     7561 gtcgcccgcg atcatcgcca aggcaatcgc cacgcggctc gacaagttag acctgccgtc
     7621 cgacgtacgt gcgcgcattg cgacgcgcat cgctgtaatc gaagcgaagg aaaaagcctt
     7681 ggcgcgtccg cgcgtcgaag ccgagcgcaa gccgtggttc tgctcgggct gcccgcacaa
     7741 cacgtcgacc aatgtgccgg aaggttcgcg tgcaatggcc ggtatcggct gtcactacat
     7801 gaccgtatgg atggaccgca gcaccagcac cttcagccag atgggcggcg aaggcgtcgc
     7861 gtggatcggt caggcgccgt tcagcaacga caagcacgtg ttcgccaacc tcggcgacgg
     7921 cacttacttc cactccggcc tgctcgcgat tcgtgcggcg atcgcgtcga aggcgaatat
     7981 cacctacaag attctctata acgacgcggt cgcgatgacg ggtggccagc cagtcgacgg
     8041 cgtgctgacc gtgccacaaa tcacgcatca gctcgccgcc gaaggcgcga cgaagatcgc
     8101 gatcgtcacc gatgaaccgg agaagtacgc cgccaacgtc ggtctcgcgc ccggcatcga
     8161 cattcaccat cgcgacaagc tcgatgaagt tcagcgtcag cttcgcgaga ttcccggcac
     8221 cacgatcctg atctatgacc agacctgtgc gacggagaaa cgccgccgca ggaagcgcgg
     8281 cacgtatccg gatccggcgc gccgtgtcgt gatcaacgaa gcggtgtgcg aaggctgtgg
     8341 cgattgctcg gtgaagtcga attgcctgtc tgtcgagccg ctcgataccg agtacggcac
     8401 caagcgccag atcaatcagt cgacctgcaa caaggacttc tcgtgcgtga acgggttttg
     8461 cccaagcttc gtatccgtcg aagggggtca gttgcgcaag ccgaaggcgg cttcgggcat
     8521 cgtcggtgac gcgatgccgc cggtgccgga gcccgaacta ccatcgattg cgcgccccta
     8581 tggggtgctg gtgacggggg tcggcggcac gggcgtcgta acgatcggcg cattgctcgg
     8641 catggccgcg cacctggaaa acaagggcgt cacggtactc gacgtcaccg gcctcgcgca
     8701 aaagggcggt gccgtgatga gtcacgtgca gatcgcccag aacccggccg acattcacgc
     8761 gacccgcatc gcaatgggcg aagcaagtct cgtgatcggc tgcgacgcga tcgtcaccgc
     8821 cagcgacgaa tgcgtctcgc gtatgcaggt gggccgcacc cgtgtcgtgc tcaatagcgc
     8881 gcacacgccg accgctgaac tcatcaagaa cccgaactgg cgcttccccg gcagtagcac
     8941 cgaagcggac gtgcgtgctg gcgcgggcga cgacggcgtc gatatggtcg acgcgaatca
     9001 cttcgctgtc gcgctgctcg gcgatgcgat ctacacgaat ccgttcgtgc tcggctacgc
     9061 gtggcaacgc ggttgggtgc cgctgaccta tcagtcgctg atgcgcgcaa tcgaactgaa
     9121 caacgtgcag atcgaaaaga accgtgccgc attcgaatgg ggccgccgcg ccgcacatga
     9181 tctggctgcg gtgcgcaagc tcgcgcagtc gcaaggacgc ggtgccgcag cggagacggc
     9241 gagcagcaag atcatctcgc tgcacacacc gaaggcgctc gacacgctga tcgataaacg
     9301 cgccgactat cttgcagcgt ggcaaaacga cgcgtatgcg aggcggtatc gggatctggt
     9361 gtcacaagtg cgtgtggcgg agtcagcgct cgattcgatc gacggccagt tgccgttgac
     9421 cgaagcggtc gcaaagaacc ttcacaagct gatggcttac aaggacgagt acgaagtcgc
     9481 gcgactctac agcgacccgg cgtttatcga aaagctaaag gcaaacttcg aaggcgactg
     9541 gaagctgcac attcacctcg cgccgccgac gttttcgaag aaagacgcgc aaggccatct
     9601 ggtgaagaag aagtacgggc cttgggtgtt cagcgccatg cacgtgctcg caaaactcaa
     9661 gttcctgcgc gggaccgcgt tcgacgtgtt cggcaagacg gaggagcgtc gcacggagcg
     9721 cgcgctgatc gtcgaatacg aagcactggt gcgtgagctg atcggcgggc tgaccgcgca
     9781 gaagcgtgaa ctggcgatcg aactggcgaa tttgccggac ggcatccgcg gctacgggca
     9841 cgtgaaggaa aataatctga agggcgtgcg ggtcaagtgg aacggattgc tgacgcgctg
     9901 gcgctcgccg gaaacgggaa agacgcagca cgcggcatga tcttgccggc ttgaccggcg
     9961 ggtatcggcc tgaaatggaa aaggcgactg tcctcgcgga cagtcgcctt ttttttgcct
    10021 gtttcgacac gtgatgcgtg tgtacaagaa caagcggtac
//
LOCUS       DATASH010000144         4045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_19843,
            whole genome shotgun sequence.
ACCESSION   DATASH010000144 DATASH010000000
VERSION     DATASH010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19843"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..528
                     /locus_tag="VHV99_04350"
                     /pseudo
     CDS             108..528
                     /locus_tag="VHV99_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422157.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1810 domain-containing protein"
     gene            780..1331
                     /locus_tag="VHV99_04355"
     CDS             780..1331
                     /locus_tag="VHV99_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634708.1"
                     /translation="MTSHRAIRNCFTLQRTCSKTSAALSCAAVLVLGGCVQPWQRFEA
                     GEPEGNVIAKLGQPREVYDLPGGGKRLMWPTQPMGEVTTAADIDAMGKVISVRQVLQP
                     SEFYRAEVGQWTKKDVLVNFGRPEETTYFPLMKREVWSYRYLEDGVWYQLFHFYFDDT
                     GVLRSTQKSPDPLHEQNGNDHVP"
     gene            1632..2894
                     /locus_tag="VHV99_04360"
     CDS             1632..2894
                     /locus_tag="VHV99_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013691039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3634709.1"
                     /translation="MPQPPADPTGGPNFSLPKHPAFRRFWCTRILSSLSFQMLAVAMG
                     WHIYALTHSAFALGLVGLAQFLPMFLLTLVVGHVADRYDRRRIAAVCQSLESAAALLF
                     AFGTFGGWISAPVIYVLAACVGAARAFESPAVASLLPAVVPRGQLPKATAWATSANQS
                     AQIAGPALGGLLYGIGPGAAYLACTLTFAAAAASVWAIPLQAKPASRAPVTLESVFSG
                     IAFIRKEPVILGALSLDLFAVLFGGATALLPVFARDILHTGPLGLGLLRSGTAIGALA
                     GTIWLAHFPLRNRPGAAMFGGVIAFGAATIVFGLSHQFFVSLFALMVLGASDTISVVV
                     RLSLVQLRTPGDMLGRVSAVNSLFIGTSNQLGEFESGVTAGWWGAQPAVLLGGVATIA
                     VALLWMRFFPELKRTRSLEREEALAPSS"
     gene            complement(2921..3799)
                     /locus_tag="VHV99_04365"
                     /pseudo
     CDS             complement(2921..3799)
                     /locus_tag="VHV99_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007732318.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
BASE COUNT          807 a         1259 c         1223 g          756 t
ORIGIN      
        1 tgccacaacc gggtagtggc caccatggac gcctgacaaa gcgggaaaca ggtgcgagaa
       61 ttgtcgtgga cgcgtggatt ctcccgccag tcaccacgga gtgccaaatg gacgatccct
      121 atgaccttca gcgcttcgtc gacgcccagg agccggtgta cgcacagggt gtgtgaagaa
      181 ctgcgtgacg ggcgtaagcg aagccactgg atgtggttcg tatttccgca gatcaagagc
      241 cttgggacaa gcgcaatggc gcagcggttc gcgatctcga cgctggccga ggccgaggcg
      301 tatctgcgtc atccgatact cggccaacgg ctgcgggaag cgacgcgact ggtcaatctt
      361 gtcaaagacc gttcgatcga ggaaattttc ggctatccgg atgacctgaa gttcaagtca
      421 tcactcaccc tgttcgcccg tgcgacaagc gacaacgaaa tttttatgga agcgctgcgc
      481 aagtacttcg gcggtgaagt cgattcgcga acgctgcagc agctataggc ggcccctgcg
      541 ctgcgcagac gcagacgcag acgcagatgc agatgcagat gcagatgcag atgcagatgc
      601 gagcgcacac gctcgggtcg aaactggaag cgctaaaggg gtcggctaag cgacgggtcc
      661 cgcgggcgcg acactgagct ggcggtcatc gtgcaaccca ttgtccaggc ggtggttgca
      721 aaacgaatct cgtgcgttcc cctcaaagat ctggcctgac ctgctgtacg gagtctgaca
      781 tgacatccca tcgagcgata aggaactgct tcacgctgca gcgtacgtgt agcaagacct
      841 ctgccgcgct gagctgtgcg gcggttctgg tgttgggcgg ctgtgtgcag ccctggcagc
      901 gcttcgaggc cggcgagccg gaaggcaatg tcatcgctaa actaggccag ccgcgtgagg
      961 tctacgacct gcccggcggc gggaagcgtc tgatgtggcc gacccagccg atgggcgagg
     1021 tcaccacggc tgctgacatc gacgcgatgg gcaaggtgat cagcgtccgg caagtgcttc
     1081 agccgagcga gttctatcgg gcggaggtcg gccagtggac gaagaaagac gtgctggtca
     1141 actttggccg tccggaggaa accacttact ttccattgat gaagcgtgag gtatggagct
     1201 atcgctatct ggaagatggg gtgtggtacc agttgttcca tttctatttc gatgacactg
     1261 gcgtgctccg ctcgacccag aaaagccccg acccgctcca tgagcaaaat ggaaatgatc
     1321 acgtcccctg accttgacga aggctaagag gatgagccaa ttgaatcatg acgttatgcc
     1381 ttcgatgtta ctcagccaac ggcacacagc tttgccacgc atcccaacca ttgagcaggg
     1441 cggaagtaac cgcccccaag gttcttcgga ctccgtgtga tccggggtgg acaggtgcca
     1501 ggcccttcgg cccccggccc gttggcacag tcttacaaaa agaaagcgcc gcgtaagtat
     1561 aaaaaacaaa taaataaact tgctctaaac taccagccgc ccgagccagc ctagtagaga
     1621 gcacgaaacc aatgccccag cctccagccg atcccaccgg cggtcccaac ttcagtttgc
     1681 ccaaacaccc ggcctttcga cgtttctggt gtacccgcat cctctcatcg ctgtctttcc
     1741 agatgctcgc cgtggcaatg ggctggcaca tctacgcgct cacgcatagc gcctttgcac
     1801 tgggcctcgt cggtctcgcc caattcctgc cgatgttcct gttgacgctc gtggtcggcc
     1861 acgtcgccga tcgctacgac cgtcgccgga tcgcggccgt ctgccaaagc ctcgaaagcg
     1921 ccgctgcatt gctattcgct ttcggcacct tcggtggctg gatcagtgcg ccggttatct
     1981 atgtgctggc cgcatgcgta ggcgcggccc gcgccttcga atctcccgcg gttgcctcgt
     2041 tattgccggc agtcgtgccg cgcgggcaat tacccaaagc cacggcatgg gctacctccg
     2101 cgaatcagtc cgcccagatc gcgggtccgg cattgggcgg cctgctctat ggcatcggtc
     2161 cgggcgcggc ctatctcgca tgcacactga cgttcgcggc cgccgcggct tccgtgtggg
     2221 ccattccgtt gcaggccaag ccggcgagcc gcgcgccggt cacgctcgaa tcggtgttct
     2281 ccggcatcgc ttttatccgc aaggagcctg tgatactcgg cgcgctctcg ctcgatctgt
     2341 tcgcggtgct gttcggcggc gcgaccgcgc tgttaccggt ttttgcacgc gacatcctgc
     2401 acacagggcc gctcggtctc ggcctgctgc gctcgggcac cgccatcggc gcgctcgcgg
     2461 gcaccatctg gcttgcccat ttcccgctgc gtaatcgccc cggtgctgcg atgttcggtg
     2521 gcgtgatcgc gttcggtgcc gccactatcg tgttcggtct atcgcatcaa ttcttcgtat
     2581 cgctcttcgc gctcatggtg ctgggcgcgt cggacaccat cagcgtcgtg gtgcgcctgt
     2641 cgctcgtgca actgcgtacg cctggcgaca tgctgggtcg cgtcagcgcg gtcaattcgc
     2701 tattcatcgg gacatcgaat caattaggcg agttcgagtc cggcgtgaca gcaggatggt
     2761 ggggcgcaca accggccgtg ctgctgggcg gcgtcgcgac gattgctgtc gccttgttgt
     2821 ggatgcggtt ttttccggaa ctcaagcgca cgcgttcgct ggagcgagaa gaggcgctcg
     2881 cgccgagcag ttgatcgatg cagcccacgc gccgcgtcgt ccatcgcacc gtcgcgagaa
     2941 tcgatgccac gacgatcaat gcgccgccga tccacgctac cgttgaaatc cgttcaccta
     3001 accagatgca ggcgaacagc gcgccgaagg ccggttcgct gcccatcagc agcgacacgc
     3061 gcgtcgggct gctgcgcttg atcgcgaagt tctgcgcgaa gaacgcgaat agcgtgcacg
     3121 caatcaccag atagccgaca taaccccaga acacggcatg cccggtgagc gatggcatcg
     3181 gctgccactg ttgcggcgca aacagcaacg caaccgcggc ggcgccgaat gccaccacgc
     3241 ccgactgaac cgcggtcacc gacaaaggcg gcaacgcgga gtcgcgcatc acccgtttag
     3301 tcacgcatac attcaacgcg cgcagcagcg cggcgagcag aatcaacgcg tcgccggaat
     3361 tgaaactcag cgcgccatcc cccgccagca gccaggcgcc gaacagcgaa aggacgaccg
     3421 ccatccattc gatgcggctc ggcctgcgtt tgagcagcca ccattcgatg agcggcgtca
     3481 gcaccacgca caggctgatt agaaacgccg cattggccgc gcgcgtcagc agaatgccga
     3541 aggtctcgca gagaaagatg ccgagtagca gggcgcccgc gatgagcacc ccacttagcg
     3601 tacggccatt ggccgcgcgc aaatggcgca acgcgggcga caggatcgca aaggtgatgc
     3661 caaagcggat tgtcagcaaa cccagcaccg gatagaacac gagggcactt ttgacgacgc
     3721 cgtaactggt gccccacacc acggcgaccg cgagcagcat cagatcggaa agcacaagca
     3781 ggcgtggttg tttcgacatg gcggggcgga cctcaagcag atgagcacgt attgcgcaca
     3841 ttgtcgaact ttgcgccgaa ggcgataatc gggtcgccgt gcacagcttt tgtgtctcaa
     3901 acgcattaat aggcgcacct tcagcgggct tctcacgatg aacccaaccg accttttcgc
     3961 catatccggc agcaaggcga aaaggtcggt tggcttctca cgatgaaccc aaccgacctt
     4021 ttcgccttgc tgccggatat ggcgg
//
LOCUS       DATASH010000145         1897 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3382273,
            whole genome shotgun sequence.
ACCESSION   DATASH010000145 DATASH010000000
VERSION     DATASH010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3382273"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..528
                     /locus_tag="VHV99_04370"
     CDS             64..528
                     /locus_tag="VHV99_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX3634710.1"
                     /translation="MAQTNVYDKLKELGIELPRAGAPAAAYVMSAQSGNTVYLSGHIA
                     KKDGNVWAGKLGATLTTEEGKLAARSIAIDLLATLHAHVGDLNRVTRIVKLMSLVNST
                     LEFTEQHLVTNGASELIADVFGERGKHARSAFGVAQIPLGACVEIELIAEVE"
     gene            709..1617
                     /locus_tag="VHV99_04375"
     CDS             709..1617
                     /locus_tag="VHV99_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEX3634711.1"
                     /translation="MSATNVRFKQVDVFTSIPFKGNPLAVVFDADGLSADQMQAIAHW
                     TNLSETTFLLKPTDPAADYRVRIFTTHGELPFAGHPTLGTAHALLESGYRPKQPGKLM
                     QQCGVGLVELQALTESGSAGEAQGGWAFAAPPARVTALAQDRYAALATALRSDAIDFS
                     ATPCAVDNGAPWLVVRVNSARDCLALEPDATALAELVHSLDTHGLAVYGPYGADGPAT
                     FEVRCLMAGGRFGVGEDPVTGSANAALAGLLSAQQLRPGVHYTARQGTVLGRAGNVSV
                     HYDDAAGKTWIGGPSVTIVDGTFRMP"
BASE COUNT          352 a          622 c          594 g          329 t
ORIGIN      
        1 aaggtctaag cgacccccgg cgcctcgccg ggttaccgaa ttcccttact gaaaggacac
       61 accatggctc aaacgaatgt gtacgacaag ctcaaggaat tgggcatcga actgccgcgc
      121 gcaggcgcgc cggcagccgc gtatgtgatg agcgcgcaaa gcggcaacac ggtgtacctg
      181 tcgggtcaca tcgcgaagaa ggacggcaac gtgtgggcgg gcaagctagg cgccacgctc
      241 accaccgaag aaggcaagct cgccgcacgc tcgatcgcga tcgacctgct cgccacgctg
      301 cacgcacatg tgggcgatct gaaccgcgtc acgcgcatcg tcaagctgat gagcctcgtc
      361 aactcgacgc tcgaattcac cgagcagcac ctggtgacca acggcgcgtc ggaactgatc
      421 gcggacgtgt tcggcgagcg cggcaagcat gcgcgctcgg cgttcggcgt ggcgcagatt
      481 ccgcttggcg catgcgtcga aatcgagttg atcgccgaag tcgaataaca cacgcccgca
      541 ggttcggccg ccgatttccc agcgagccgt cctgaaggta ctgaacgcgt cgcgaggtgt
      601 ctgtcacctg cggcgcgttt tgcatttgtg gcgagttggc tcgttccttt atcaccagat
      661 agaattcgcg aacccttccc ctctgccggc ttcgccggat cgcgcccgat gtccgccacc
      721 aacgttcgtt tcaaacaggt cgacgtgttt acatcgatcc cgttcaaagg caatccgctc
      781 gcggtcgtgt tcgacgccga tggactcagc gcggatcaga tgcaggccat cgcgcactgg
      841 accaatctgt ccgaaaccac cttcctgttg aaaccgaccg acccggccgc cgactatcgc
      901 gtgcgtattt tcacgacgca tggcgaatta ccgttcgccg gtcatccgac actcggcacc
      961 gcgcatgcgt tgcttgaaag cgggtatcga ccgaaacagc ccggcaagct gatgcagcaa
     1021 tgcggcgtgg gtctcgttga attgcaggcc ctgactgaat ccggctcagc cggcgaggca
     1081 caaggcggat gggctttcgc cgcgccgccc gcgcgcgtga cggcacttgc acaagatcgc
     1141 tacgcggcac tggctacggc tttgcgtagc gacgcgatcg atttcagcgc aacgccctgc
     1201 gcggtcgaca atggtgcgcc atggctcgtg gtgcgcgtca attcagcgcg cgactgcctc
     1261 gcgctcgaac ccgatgccac cgcactcgcc gaactcgtgc attcactgga tacccatggt
     1321 ctcgcggtct acggcccgta cggcgctgac ggtccggcca ccttcgaggt gcgctgcctg
     1381 atggcgggtg gtcgcttcgg cgtcggcgaa gatccggtaa cgggcagcgc gaacgccgcg
     1441 ctcgcgggtc tcctgagcgc gcagcagttg cgccccggcg tgcactacac ggcccgccag
     1501 ggcacggtgc tgggccgcgc cggcaacgtt tcggtgcact atgacgacgc agccggcaag
     1561 acgtggatcg gcgggccgtc agtgacgatc gtcgacggca cgttccggat gccgtgaacg
     1621 gcgcggcgtg gcgtggcgca ccatggcagg ccagcgaaca ctagtctgcc gctgaacgca
     1681 gcacgtgttg cgctcctttg ggccgccctt gagcacaaaa cgccgcagcg tctgcacgat
     1741 taatgcgccc cgcatgatga gaggctggca cacctgaatt ccggaccccg atgaaatatt
     1801 cgaaaacgac tgccaggctg cggcaccaag gctggccgcg tcgcctggaa attcagccgg
     1861 ctgggacggt ctgtcgcgcc cgcgtatacc cgatgcg
//
LOCUS       DATASH010000146         3654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3186104,
            whole genome shotgun sequence.
ACCESSION   DATASH010000146 DATASH010000000
VERSION     DATASH010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3654
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3186104"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..1213
                     /gene="fabV"
                     /locus_tag="VHV99_04380"
     CDS             17..1213
                     /gene="fabV"
                     /locus_tag="VHV99_04380"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018434558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabV"
                     /protein_id="HEX3634712.1"
                     /translation="MIIKPRVRGFICVSTHPTGCEANVKEQIEYVKARGPIANGPKNV
                     LVIGAYTGYGLAARISAAFAADAATLGVFFERPGSETKAGTAGWYNTAAFEKLATEKG
                     LYATSINGDAFSDEVKQRTIEVIKRDLGRVDLVVYSLAAPKRTHPKTGEVFNSTLKPI
                     GKAVNLRGIDTDKEVVKETVLEAATQAEIDNTVAVMGGEDWQMWIDALLEAGVLADGA
                     KTTAFTYLGEQITRDIYWNGSIGAAKKDLDQKVLGIREKLAAKGGDARVSVLKAVVTQ
                     ASSAIPMMPLYLSLLFKVMKKKGTHEGCIEQVYGLYKDSLYGATPHLDEEGRLRADYK
                     ELDPEVQGRVQELWTQVTNDNIYELTDFSGYKTDFLRLFGFEIAGVDYEADVNPDVQI
                     PRLVQL"
     gene            complement(1342..1986)
                     /locus_tag="VHV99_04385"
     CDS             complement(1342..1986)
                     /locus_tag="VHV99_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX3634713.1"
                     /translation="MSRSTAAQRLKPRKAPVQRRSVQTVEAVLEAAARILETHGLAAY
                     TTNAVAERAGVSIGSFYQYFPNRDALTVALIERETAQLMAEIESATLVEHSRGCVLAM
                     VRAAVAHQMRRPVLARIIDFEERRLPLGERDRRVADTIHAALLAALTDKPEAPDLDDA
                     NLVAHDLLAIVHGMVDAAGEREERDAAALEVRVMRAVDGYVALSRREKERGDSL"
     gene            2086..2445
                     /locus_tag="VHV99_04390"
     CDS             2086..2445
                     /locus_tag="VHV99_04390"
                     /inference="COORDINATES: protein motif:HMM:NF028384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="darcynin family protein"
                     /protein_id="HEX3634714.1"
                     /translation="MTLSVKEIVLTVFMLVKTTPEWLGFSVDERFRLLREHVEPILET
                     HADEVRLRFYDVEFYSARVTDIWVWDATSHHAYELLVEALRETPFWDRFFEIVEILPG
                     VENAFARNDKRDALAAS"
     gene            2636..3016
                     /locus_tag="VHV99_04395"
     CDS             2636..3016
                     /locus_tag="VHV99_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634715.1"
                     /translation="MKSLLVLAVMMLPALAAMAQTTGSTAFPDDAVAASASDIQQRLA
                     GKTFNIKLADGSLWHVQYSTDGAFDFSTNKGFTDKGEWKAEDGKVCSKGRKIGSSCNE
                     IRTKGDAIFLKRDNGEVVEFVEQH"
     gene            complement(3092..3436)
                     /locus_tag="VHV99_04400"
     CDS             complement(3092..3436)
                     /locus_tag="VHV99_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018417984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634716.1"
                     /translation="MRVSKFALALLTAGFTLNASAEMTASQYKLWAHTDNNSVYAAYI
                     TGTINALGWANGDLVSKKRPPLFCPPENLAIGNQNVYPLLDQFFTNHPGISDDFPVGL
                     AILRTLQAAFPC"
BASE COUNT          789 a         1135 c         1068 g          662 t
ORIGIN      
        1 cgacaagagg tgcttcatga tcatcaaacc gcgcgtgcgt ggcttcatct gtgtatcgac
       61 ccatccgacc ggctgcgaag ccaacgtcaa ggaacaaatc gaatacgtca aggcacgcgg
      121 tcccatcgcc aacggcccga aaaatgtgct cgtgatcggc gcatacacgg gctacggcct
      181 cgccgcgcgc atcagcgcgg cattcgccgc cgacgccgcc acccttggcg tgttcttcga
      241 acgccccggc agcgaaacca aagcaggcac cgccggctgg tacaacaccg ccgcgttcga
      301 aaaattggcc acggaaaaag gcctctacgc gaccagcatc aacggcgacg ccttctccga
      361 cgaagtcaag caacgcacca tcgaggtcat caagcgcgat ctcggtcggg tcgatctcgt
      421 cgtctacagt ctcgcggcgc ccaagcgcac gcacccgaaa accggcgagg tgttcaactc
      481 gaccctgaag ccgatcggca aggccgtcaa cctgcgcggc atcgacaccg acaaggaagt
      541 cgtcaaggaa accgtcctcg aggccgccac gcaagctgaa atcgacaaca ccgtcgcggt
      601 gatgggcggc gaagactggc aaatgtggat cgacgccctg ctcgaagcgg gcgtcctcgc
      661 cgacggcgcg aagacaaccg cgttcaccta tctcggcgaa cagatcacgc gcgacatcta
      721 ctggaacggt tcgatcggcg cggccaagaa agatctcgac cagaaggtgc tcgggatccg
      781 cgagaaactc gcggccaagg gcggcgacgc gcgcgtctcg gtgctcaagg cggtcgtcac
      841 gcaagcgagc tccgcgatcc cgatgatgcc gctgtacctg tcgctgctct tcaaggtcat
      901 gaagaaaaaa ggcacacacg aaggctgtat cgaacaggtc tacgggctct acaaagacag
      961 cctgtacggc gccacgccgc acctcgacga agaaggccgt ctgcgcgccg actacaagga
     1021 actcgatccc gaggtgcaag gccgcgtcca ggaactctgg actcaggtga ccaacgacaa
     1081 tatctacgaa ctcaccgact tcagcggcta caagaccgac ttcctgcgcc tgttcggatt
     1141 cgagatcgcc ggcgtggact atgaagcgga cgtcaatccg gacgtgcaga ttcccaggct
     1201 cgtgcaactt tgaggtttgc acgcggggcg ctttcggccc cgcacgccgg tccgattccg
     1261 accatgttgc gttagccggg ccacacgggc cggtccggtt cggtcatggt tgcgttagcg
     1321 attcccgcag gctcgcgccc gctacaagga gtcgccacgc tctttctccc gccgcgacag
     1381 cgccacgtag ccatctacag cgcgcatcac ccgcacttcc agcgccgcgg cgtcgcgttc
     1441 ctcgcgctcg cccgcagcgt cgaccatgcc gtgcacgatc gccagcaaat cgtgcgcgac
     1501 aagattcgca tcgtccagat cgggcgcttc gggcttgtcg gtcagcgccg ccagaagcgc
     1561 ggcatgaatc gtgtcggcaa cccggcgatc acgctcgccg agcggcaatc tacgttcctc
     1621 gaaatcgatg atgcgcgcca gcaccggcct gcgcatctga tgcgctaccg ccgcgcgcac
     1681 catcgcgagc acgcagccgc gcgaatgctc caccaacgtt gccgattcga tctccgccat
     1741 cagttgcgcg gtttcccgtt cgatcaacgc gaccgtcaac gcgtcgcgat ttggaaagta
     1801 ttgatagaac gacccgatac tgacgccggc tcgctccgcg accgcgttgg tcgtgtacgc
     1861 agcaaggcca tgggtctcca gaatgcgagc cgccgcttcg agcacggctt cgacggtttg
     1921 taccgaacgc cgctgaaccg gcgctttacg gggtttcagg cgttgcgctg cagtcgatct
     1981 ggacatggcc gaaatgcgag ttttgaatgt gagcgatcgc tcgtattcta aacctgtttc
     2041 gcgaaacctc agcgaccgct cagccatcaa ctggaatcga acgacatgac tttatctgtg
     2101 aaagaaatcg tattgaccgt attcatgctt gtcaaaacaa cgcccgaatg gctcggcttt
     2161 tccgtcgacg aacgttttcg cctgctgcgc gaacatgtcg agccgatcct cgaaacgcat
     2221 gccgatgagg tcaggctgcg tttctacgac gtcgagttct attcggcgcg cgtgacggac
     2281 atctgggtgt gggacgcgac gagccatcat gcgtacgaac tgctggttga agcgttgcgt
     2341 gagacgccgt tctgggaccg cttcttcgaa atcgtcgaga ttctgcctgg cgtggaaaac
     2401 gcctttgcga ggaacgacaa gcgggatgcg cttgcggcgt cgtgaccggc atggtgtcgc
     2461 gaatggacga gccggcgcgt ttcagttgaa tcggcgccgg tctgtgcgtt atgccacaca
     2521 tcgatccatg cgaccggtgt agcgtcggcc agcggacagt ggcccatttc cttccgaaat
     2581 ggcgtccaaa atgccgtccg aaagccagcc gttgccaata acgcggagac actcaatgaa
     2641 atccctgctc gttcttgccg tcatgatgct gccggctctc gctgcaatgg cgcagaccac
     2701 cggttcaacg gcattccccg acgatgccgt ggcggcatcg gcctccgaca ttcagcaacg
     2761 gctcgccggc aagaccttca acatcaaact cgctgacggg agcctctggc acgtccaata
     2821 cagcaccgac ggcgcattcg acttcagcac caacaaaggc tttacggaca agggggaatg
     2881 gaaagcggaa gacggcaagg tatgcagtaa aggccgcaag atcggcagtt cctgcaatga
     2941 aatccgcacc aagggcgacg cgatctttct gaagcgcgat aacggcgagg tggtcgagtt
     3001 cgtcgagcag cattgaatcc cgtcgaaccg ccgggctgcc gacagcggtc acgaggcagc
     3061 gtcggcgaga tgtcgccgcg ctgcgccagg atcaacaggg aaacgccgcc tgaagcgtgc
     3121 gcagaatagc gaggccgacg ggaaagtcgt ccgagatgcc cggatgattg gtgaaaaact
     3181 gatcgagcag cggatagacg ttctgattgc cgatcgccag gttttccggc gggcagaaca
     3241 gcggcggacg cttcttcgac accagatccc cgttcgccca ccccagcgcg ttgatcgtgc
     3301 cggtaatgta agccgcgtag accgagttgt tgtcggtatg agcccagagc ttgtattgcg
     3361 aggccgtcat ctcggcactt gcattgagcg tgaaaccggc cgtaagcaaa gccagtgcaa
     3421 atttcgatac ccgcatcttt gaaccttcaa tctgtgtcgc aaaagcggcc attgtaggtg
     3481 agtcggatca tcgtgcgcgc aaacgctcag tgtccagatt tttatcaaat actttgcaag
     3541 tatctagaag ctattttgaa agaatcggcg agcgaagcag gcccctcatg ccagagccgc
     3601 cgccatgatt ttatggcgtg aagaatgcag cacgaatgcg ctgttcacgg cgtg
//
LOCUS       DATASH010000147         3307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3186284,
            whole genome shotgun sequence.
ACCESSION   DATASH010000147 DATASH010000000
VERSION     DATASH010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3186284"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2398
                     /locus_tag="VHV99_04405"
     CDS             <1..2398
                     /locus_tag="VHV99_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006046922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX3634717.1"
                     /translation="IECAVVIAEYLSGEEGYDVTSNFMGPFSLHAVMALALNVPANRL
                     RHKAPRDSGGSFGVKQAVFPYVVLMCLASRKANAPVKYVEDRLEHLSAATSATARLTT
                     LEAAVENDGRITALNYDQIEDCGGYLRAPEPATFYRMHGCLTGAYAIPALLVRNRVVL
                     TNKTPTGLVRGFGGPQVYFALERLMQRIAIELKLDVLDVYRRNFVSADAFPYRAAAGA
                     LLDSGNYQEALRRSLAEGGYDELKARREAARTEGRLYGIGFAAIVEPSVSNMGYITTV
                     MPAEARKKAGPKSGAIASATVSVDLLGGVVVTVASTPAGQGHMTVCAQVVADVLGLAP
                     HEIVVNVEFDTHKDAWSVASGNYSSRFAGAVAGTVHLAAMRVRDKLARIVSSQLACKP
                     GDIRFEGGRIYKAGDETHAMPFGRVASNAPHWAPALLPEGEEPGLRETVFWTPENMDA
                     PDEQDRINTSAAYGFAFDMCGVEVDRATGRVRIDRYVTAHDAGTLLNPALADGQIRGA
                     FAQGLGAALMEEFRYGADGSFQSGTLADYLMPTTCEVPDPVIIHLETPSPFTPLGAKG
                     LGEGNNMSTPPCIANAVADALGVDDIRLPLTPSKVMALVGIEDPEPSRPEFREAQQKK
                     PVQPGGKALTAQGSVDLAASPEAIFAVLLDPNALAKVIPGCHALEANGENSYRADVTV
                     GVGMIKARFEAQITLSDLDPPHRLRLAGAGMSSLGSARGNGLVELTPAGTGTRLTYDY
                     EAQVSGKVAAVGGRMLEGAAKVVLRQLFESLGRQAAGKPLKTGGSWLGRLVASFRRKS
                     "
     gene            2395..3210
                     /locus_tag="VHV99_04410"
     CDS             2395..3210
                     /locus_tag="VHV99_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX3634718.1"
                     /translation="MKPAPFDYLRAVSTHDALDTLAQTGEDARILAGGQSLMAVLNMR
                     LAQPRVLIDISRTEELDRVNIEHGFLVVNAAATQGSVEWRPSLQNEVPLLALAFPHIS
                     HFQIRNRGTVCGSIAHADPSAELPLVLSVLGGDVMLRSKKRKRVLKAGDFFQGMLMTA
                     REPDELVEGVRFPLKRAGERYAFAEFSARHGDFAMVACATVVTDTSIRIAVGGVADRP
                     VVEQWPRLHGDDLRGALNDLSWKLNAQDDTHVSATYRRHLVRQLGWRVIEEAK"
BASE COUNT          606 a          977 c         1116 g          608 t
ORIGIN      
        1 gatcgaatgt gccgtggtta tcgccgagta tctttccggt gaagaagggt atgacgtcac
       61 ctcgaatttc atggggccgt tctcgctgca cgcagtgatg gcgctcgcgc tgaacgtgcc
      121 ggccaaccgc ctgcgtcaca aggcgccgcg cgattcgggc ggcagcttcg gcgtgaagca
      181 ggccgtattt ccctacgttg tactgatgtg cctggcctcg cgcaaggcaa acgcaccggt
      241 gaaatacgtg gaagaccggc ttgagcacct gagcgcggcg acctccgcca cggcgcggtt
      301 gaccacgctc gaagcagccg tggaaaacga tggccggatc accgcgctca attacgacca
      361 gatcgaggat tgcggcggtt atctgcgcgc gcccgagcct gccacgtttt atcgcatgca
      421 cggctgttta acgggtgcct atgccatccc cgcgctgctg gtgcgcaata gggttgtgct
      481 gaccaacaag acgcctacgg gcctcgtacg cggtttcggc gggccgcagg tttatttcgc
      541 gctcgagcgc ctgatgcaac gcatcgcgat cgagttgaag ctcgacgtgc tggatgtgta
      601 ccggcgcaat tttgtttccg ccgatgcctt cccttatcgc gccgcagcgg gggcattgct
      661 ggactcgggc aactatcagg aagcgttgcg acgttcgctc gccgagggtg gttacgacga
      721 gctcaaggca cgccgcgaag ctgcacgcac ggaaggccgt ttgtacggca tcggctttgc
      781 ggcgattgtt gagccgtcgg tatcgaacat ggggtacatc acgacggtga tgcccgctga
      841 agcacgcaag aaggcaggac ccaagagcgg tgccatcgcg agcgcgacgg tcagcgtcga
      901 cctgcttggc ggcgtggtgg tgacggtggc gtcgacaccg gccgggcagg ggcacatgac
      961 cgtatgcgcg caagtggtgg cggatgtgct cgggctcgcg ccgcacgaga tcgtggtgaa
     1021 cgtggagttc gatacgcaca aggatgcgtg gtcggtggcg tcgggaaatt attcgagccg
     1081 ctttgccggc gcagtcgcgg gcacggtgca tctcgcggca atgcgcgtgc gtgacaagct
     1141 ggcgcgcatt gtttcatcgc agctcgcgtg caagcccggc gacatccgtt ttgaaggcgg
     1201 acgaatctac aaggcaggcg acgaaacgca tgcgatgccc ttcggccgcg tagcaagcaa
     1261 tgcaccgcat tgggcgcccg cgttgctgcc cgaaggcgaa gaaccgggtc tgcgcgagac
     1321 ggtgttctgg acgcccgaaa acatggatgc acctgatgag caggaccgca tcaatacatc
     1381 ggcggcgtat ggatttgcct tcgatatgtg cggcgtggag gtggaccggg caacagggcg
     1441 cgtgcggatc gaccgctatg tgaccgcgca cgatgcgggc acgttgctga accccgcgct
     1501 tgccgacggc cagattcgcg gtgcgttcgc gcaggggctc ggtgcggcgc tgatggaaga
     1561 gtttcgctat ggcgccgacg gtagtttcca gtccggcacg ctggccgatt acctgatgcc
     1621 gaccacgtgc gaagtgcccg atccggtgat cattcatctg gagacgccat cgccgtttac
     1681 accgctgggc gccaagggct tgggcgaagg caacaacatg agcacgccgc cttgcatcgc
     1741 gaatgcggtg gccgacgcgc tcggtgtcga tgacatccgt ttgccgctca caccttcgaa
     1801 ggtgatggcg ctggtcggta tcgaggaccc cgaaccttcg cggcccgagt tccgggaggc
     1861 gcagcagaaa aaaccggtcc agcctggcgg caaggcgctg accgcgcaag gcagcgtgga
     1921 tctcgctgca tcgccggaag caatcttcgc ggtactgctc gacccgaacg cgctcgccaa
     1981 ggtgatcccc ggttgtcatg cgctcgaggc gaacggcgaa aattcttatc gcgcggatgt
     2041 gaccgtgggc gtgggaatga tcaaggcgcg cttcgaagcg cagatcacgc tgtccgatct
     2101 cgatccgccg caccggttgc gtctcgctgg cgcggggatg tcgtcgctcg gtagcgcgcg
     2161 tggcaatggc ctcgtggaac tcacgcccgc cggtaccggc acgcggctca cctatgacta
     2221 cgaggcacag gtctcgggca aggtcgcagc cgtgggcgga cggatgcttg aaggcgccgc
     2281 gaaggtcgtg ttgcgccaac tgtttgaatc actgggacgt caggcagcag gcaagcccct
     2341 gaaaacgggt ggttcttggc tgggccggct cgtggcaagc ttcaggagaa agtcatgaag
     2401 ccggcgccat tcgattatct gcgcgccgtt tccactcacg acgcgctcga caccctcgcg
     2461 caaaccggcg aagacgcacg cattcttgca ggcggccagt cgctcatggc cgtgctgaac
     2521 atgcggctcg cgcaaccgcg ggtgctgatc gatatctctc gcacagagga actcgatcgc
     2581 gtgaacattg agcacggttt tcttgtggtg aacgcagcgg ctacccaagg cagcgtggaa
     2641 tggcgtccgt cgcttcagaa cgaagtgccc ttgctcgcgc tcgcgttccc gcacatttcg
     2701 cattttcaga ttcgcaatcg cggcaccgta tgcgggtcaa tcgcgcatgc cgaccctagc
     2761 gcggagctgc cgctggtgct ctcggtgctc ggcggcgacg tcatgttgcg gtcaaagaaa
     2821 cgcaagcgcg tgctcaaggc gggagacttt tttcagggca tgttgatgac cgctcgtgag
     2881 cccgacgaac tggttgaagg cgtgcggttc ccgctgaaac gggccggcga gcgatacgcg
     2941 tttgccgaat tttctgcccg tcacggcgac ttcgcgatgg tcgcgtgcgc caccgtagtg
     3001 accgacacat cgatcaggat cgccgtaggc ggtgtggcgg accggccggt ggtcgagcaa
     3061 tggccaaggc tgcatggcga tgacttgcgt ggcgcgctga acgacttgag ctggaagctg
     3121 aatgcgcagg acgacaccca tgtcagcgca acgtatagac gccatctggt gcggcaactc
     3181 ggatggcgcg tgatagagga ggcgaagtga gcaagacatc atcaaccacg cttgcgcgcg
     3241 gcgagcgccg gcgcatcacc gtgacgctca acggacgcga gcgtacgggc tattgcgaat
     3301 cgcgcga
//
LOCUS       DATASH010000148        13602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_20496,
            whole genome shotgun sequence.
ACCESSION   DATASH010000148 DATASH010000000
VERSION     DATASH010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 13602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13602
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20496"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="VHV99_04415"
     CDS             <1..631
                     /locus_tag="VHV99_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX3634719.1"
                     /translation="TMVPRAIASVKERYPQLQIWAEVETSDVMLPRLAEGELDIMIGR
                     VLERQNQFKAEVRYEPLADEPLCVVARPGHPLENETGLTLRGIVNASWVLHPPGSVLR
                     HRFDLMFSQIGLNPPQNVVNTNNFLAISSLLLQSDMLAVLPDEVARQYQQYGVLQRVP
                     IDLPCRMDTFGIITRQSHLLSPAAAVVLDALREAAAEVYGTAFEPAVAR"
     gene            783..1340
                     /locus_tag="VHV99_04420"
     CDS             783..1340
                     /locus_tag="VHV99_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009908298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HEX3634720.1"
                     /translation="MALKSTIYKAELQIANMDRHYYADHSLTIARHPSETDERMMVRL
                     AAFALFAQERLEFCKGLSDIDEPDLWQKDLTGAIETWIEVGQPDERRIAKASGRSNEV
                     IVIAYGGRTSDIWWQGVRSKVERMRNVTVWTLGEDIAAALGKLAERTMRLQCTVQDGE
                     AWLGSAEADAVKIEWTVLKAPANAW"
     gene            complement(1444..1869)
                     /locus_tag="VHV99_04425"
     CDS             complement(1444..1869)
                     /locus_tag="VHV99_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018418913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3634721.1"
                     /translation="MRIHIREIDHIVIRASNVPDMTRFYCDVLGCSVEKEQPDLGLTQ
                     LRAGRSLIDLLKVGAKLDHPENGVPGAGRNMDHLCLRVDPFDAEALKAHLAEHGARPG
                     ELALRYGADGFGQSLYLFDPEGNMVELKGPPEAARVTSL"
     gene            2264..3451
                     /gene="ada"
                     /locus_tag="VHV99_04430"
     CDS             2264..3451
                     /gene="ada"
                     /locus_tag="VHV99_04430"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HEX3634722.1"
                     /translation="MNRTDRTETGDTGSETAVGPSRWSTDADRWEAVTRREPQADGAF
                     FYAVKTTGVFCRPSCASRQPRRENVAFFTDANAARAAGYRDCKRCQPGGLPRELEIVN
                     RACAALDADPQQRLTLAQLSDAVHVSPFHLQRLFKRVVGVSPRQYQAAQRGAALRDAL
                     QSGSDVTRATLDAGFGSPSRMYDSASAELGMAPSAFRRKGAGLTVRYASASTSLGFVL
                     VAATDKGICKIGFGDDAAILADELRGEFANADLVEDATRLAPFIAQIDAYLRGTRQDF
                     ELPLDIAATAFRQRVWDALRRIPYGETRSYSQIAEAVGAPRAVRAVASACATNPVALA
                     IPCHRVVQKGGALAGYRWGLPRKAALLDNEAQHAGDVSLAERDRRERAALTATTTNLD
                     HAA"
     gene            3448..4365
                     /locus_tag="VHV99_04435"
     CDS             3448..4365
                     /locus_tag="VHV99_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX3634723.1"
                     /translation="MSTLDDVATLELPFKAPFDWLRLLRFFGGRATPGVETVEDGAYR
                     RAIDWTGDSGTLTVRLHPRKRCIVANIDGPASRHADALAAPIARMFDLHADPKKIGGE
                     LAADPWLAPLIEAVPGLRVPGAWSGFELVVRAIVGQQVSVKAATTIIGRLVQRAGERI
                     EGHPHENTAWRFPTPAALAAVDLDKIGMPGKRVAALQGFAQAVASGDVPLDSGVADAD
                     GLRAALLALPGIGPWTVEYVAMRAWRDADAWPAWDLVLMQSICARDPSLVRPTQQRTR
                     TDAWRPWRAYAAMHLWNEVADRAGAARGG"
     gene            4908..6524
                     /gene="gshA"
                     /locus_tag="VHV99_04440"
     CDS             4908..6524
                     /gene="gshA"
                     /locus_tag="VHV99_04440"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEX3634724.1"
                     /translation="MPNTTPSRTTDAFSHRLSVLTSGPQRDALIRGLRGIERESLRVT
                     HDGQLAMTPHPRALGSALTHPSLTTDYSEALLELITPAEHDVAITLENLDTLHRFVYA
                     ELGDEILWNNSMPGLLPETDDGIPIAHYGTSNIGKLKYVYRIGLALRYGRTMQCIAGI
                     HYNYSLNEEVWRVLHADQQSTANAVDFQSDRYLALIRNFRRTNWLLMYLFGASPALDR
                     RFLRDREHTLETLDADTLYRPYATSLRMSDLGYSNTTAQAALHADYDTLPGYLDALAK
                     AVSQPYPAYEAIGTQRDGEWVQINTNVLQIENEFYSTIRPKRITHPGERPLHALAARG
                     VQYVEVRCMDIDPFEPTGISLETSRFLDAYLLVCALDESAPLPPHAYAEANQNFGRVT
                     MEGRKPGLELTRDGSPIAMTDWANELLAKIDAAAATLDALHGGDAHARAVAVQRTKLA
                     DASLTPSARVLQTMRDKQQSFLAFALEQSEVHAAYFRSRPLDAAQTKEFTDLAAQSLA
                     EQAKLEREEVGSFDAFVAAYRAYTLNRFSV"
     gene            7120..7620
                     /locus_tag="VHV99_04445"
     CDS             7120..7620
                     /locus_tag="VHV99_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634725.1"
                     /translation="MKKDLLAVLCVAAVSAAGCSTQGQVNPDVMQIATTPLTCSNKAE
                     CDIWWQRAQTWVTSHSKYKVESASDTLIQTAGPDGGKRALAYQITRTANPDGTATIGF
                     AAHCDGSLGCKPNPWEAGADFKEYVRGAGAPQSGANPTQPAPTAMHPDAAGQSIPGTS
                     GEAITQ"
     gene            complement(7651..8454)
                     /locus_tag="VHV99_04450"
     CDS             complement(7651..8454)
                     /locus_tag="VHV99_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634726.1"
                     /translation="MNKFENLFRLGAATLLLAFSTIGQAQIPVNIGQVSSSTSLVPEQ
                     LLNSMEFYAQAVSNSVKTAPPSSTKIKLLASKWRSATIARFEYTYGGANGESNTRVYN
                     AISGQDPQDILYDAGSAPHRQLNAFLQGGRVEKAARNIEVPEGRLAANIDKQAADAEL
                     KALKNFENDILHNGLPRGGRLVGYISQAPCDSCGNVLKQQLPATGYAAEVEVSYLPNA
                     KAPGISETEENIAQEFNARRLVYKDNILSLRARALGKLESAGGQTAGCL"
     gene            complement(8480..9661)
                     /locus_tag="VHV99_04455"
     CDS             complement(8480..9661)
                     /locus_tag="VHV99_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634727.1"
                     /translation="MKTLRMNSRPLASSNQFFRLVKTIGLAWSCLTLTSVQPAVAQQK
                     FFADTLTKSVIYHGVKSGLGESITNANVDQDFLVAPDSVYSQMKSQPTAINTMEWNLA
                     LDDFNHDNRVSLTLADSVKLFANENGLTKARPDIEINMSRRRPGYRAEESNLVVANAT
                     KAGVDGDIFYKSIFIFGSKYITIAAKFAVAAQILRDSARQVLPAEQSRNGIRKDVLDR
                     YLSTAVTNVHNISEYDKNYLMNLLHRQLRSSDYQSEKFKYGEFTPPAQFRIARLAAAY
                     QDAQGYIGHPPCSIDGKSQNPENSGNLCFTDMTDKALYSWYKNRYAAEMSPYRPIDSG
                     SSMEKLARILLPIAMIMDGLAAVEFFAALEATELGAEEAFTEEEVRASEARYLDRFCP
                     A"
     gene            complement(9970..11532)
                     /locus_tag="VHV99_04460"
     CDS             complement(9970..11532)
                     /locus_tag="VHV99_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004525778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX3634728.1"
                     /translation="MAATAPVDALPAAQPAPLKGGTLALLTVGLALGTFMEVLDTSIA
                     NVAVPTISGSLGVATSQGTWVISSYSVASAIAVPLTGWLARRVGEVRLFTLSVLLFTI
                     ASALCGFAHNFESLIAFRLLQGLVSGPMVPLSQTILMRSYPPEKRGLALGLWAMTVIC
                     APIFGPVMGGYITDNYTWPWIFYINVPIGLFSAVCAFLLLRGRETKVTKQRIDAIGLT
                     LLVIGVSCLQMVLDLGKDRDWFNSTFIVTLTIIAVVSIAFLLVWEMTDKEPVVDLSLF
                     KDRNFALGVLIISFGFMAFFGSVVIFPLWLQTVMGYTAGIAGLATAPVGILALFLSPL
                     IGKNMHRLNLRVVASFAFIVFAFVSFWNSTFTLDVPFNHVIWPRLVQGIGVACFFVPM
                     TTITLSSVSDERLASASGLSNFFRTLSGAIGTAISTTYWENDTIYHHAMLTDSVNVYA
                     AGTNAYTSALAALGFSGDGVTAQLNQVVTAQAYMMATNDFFRISCAAFLVLAGLVWVT
                     KPRKGVGASMGH"
     gene            complement(11666..12208)
                     /locus_tag="VHV99_04465"
     CDS             complement(11666..12208)
                     /locus_tag="VHV99_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX3634729.1"
                     /translation="MTDGPYNADEIHLESSLGYYLTKARNVLVERMDRAVNPLGLTAQ
                     QIGVILLLSAQRASTPFELSRVMSYDSGSMTRLLDRLEKKGFVVRSRSDADRRMVKLE
                     LTQQGREAAQQLPALGAAVLNEQLRGFSAADHATLLDLLGRFIANGIGGGASADCGLG
                     APQESQEASPEEPPAELGAQ"
     gene            12463..12825
                     /locus_tag="VHV99_04470"
     CDS             12463..12825
                     /locus_tag="VHV99_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634730.1"
                     /translation="MLYLKNTLGGLSRSLTDSALSSFQGPHRWWKLALLTVLFVLPGG
                     SVGVALFAWVEHRRGRKGAQAAPEESVGSAVAATSAVAATTAATRSSSACHGRLCAPC
                     RAAAGKLARQTTSADSRV"
     gene            13076..>13602
                     /locus_tag="VHV99_04475"
     CDS             13076..>13602
                     /locus_tag="VHV99_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX3634731.1"
                     /translation="MQFDRISRARALAPRALTIAVAAALGTLLAGCAVGPDYKRPAAE
                     IPASYKEAAPGWKVAQPADQQDRGDWWTLYEDPQLNTLEDKLNTSNQTIAQFAAAYRQ
                     ARALVSEARSAYFPTVGASIGATRSGNGSSSNGTPTSVSSRSGVSNSYNVQLDASWEP
                     DLWGSVTRSVNAQKAG"
BASE COUNT         2851 a         4124 c         4071 g         2556 t
ORIGIN      
        1 gacgatggtg ccgcgcgcga tcgccagcgt gaaggaacgt tatccgcagt tgcaaatctg
       61 ggcagaggta gaaacctcgg acgtgatgtt gccgaggctg gccgaaggcg aactcgacat
      121 catgatcggc cgggtgctgg agcggcagaa ccagttcaaa gcggaagtcc gctatgagcc
      181 gctcgccgac gaaccgctct gcgtggtcgc gcggcccggc catcctctcg aaaatgagac
      241 aggtttgaca ctgcgcggaa tcgtcaacgc cagctgggtt ttgcatccgc cgggcagcgt
      301 gctgcgccat cgcttcgatc tgatgttttc gcaaatcgga ctgaatccgc cgcaaaacgt
      361 ggtcaatacc aacaattttc tggcgatttc gagcttgctg ctgcaaagcg acatgttggc
      421 ggttttacct gacgaagtgg cgcgtcaata ccaacaatat ggcgttttgc aacgcgttcc
      481 aatcgatttg ccgtgcagga tggatacatt cggtatcatc acgcgccagt cgcatctgct
      541 ttcgccggct gccgccgtgg tcctcgacgc cttgcgggaa gccgccgccg aggtctacgg
      601 gacagctttc gagccggcag tggcgcgata aatgcttctc ccggtgcgac agtcgcaatt
      661 gcagccagac gtcggctcgg gaggaacaca agatgtacaa cacacacgga aaacggcaac
      721 gagccgtcga cgactctttt tctgttcgct ccggtggagc gagcgcctgg tttagtaacg
      781 gcatggctct caaatcgacg atttacaaag cggaactcca gatcgccaac atggaccggc
      841 actactacgc cgaccattcc ctgacgatcg cccgccatcc atcggaaacc gacgaacgga
      901 tgatggttcg gctcgccgcc ttcgcgctgt tcgcgcagga acgccttgaa ttctgcaagg
      961 gtttgtcgga tatcgacgag ccggatttgt ggcagaagga cctcaccggc gcgatcgaaa
     1021 cctggatcga agtcggccag ccggacgaac gccgcatcgc caaggcgagc ggccgctcga
     1081 acgaggtgat cgtgatcgcc tatggtgggc gcacctcgga tatctggtgg caaggcgtgc
     1141 gcagcaaggt ggaacgcatg cgcaatgtga cggtatggac actcggcgaa gacatcgccg
     1201 cggcgctcgg caagctcgcc gagcggacca tgcgtctgca atgcaccgtg caggacggcg
     1261 aggcatggct cggcagcgca gaggccgatg cggtgaaaat cgagtggacg gtgttgaagg
     1321 cgcccgccaa cgcctggtag cgtcatgttg ccgctggggt cgtctgcggc ttgtgcgcgc
     1381 gtctgcctgt cttgacggca gccgcctgtt acatcattcg ccgcaaggct gcggccattc
     1441 ccattacaac gacgtcacac gcgccgcttc gggcggccct ttgagttcga ccatattgcc
     1501 ttccgggtcg aacagataca gcgactgccc aaatccgtcc gcgccgtagc gcaaagccag
     1561 ctcgccgggc cgcgcgccgt gttcggccag atgcgccttg agtgcctcgg cgtcgaaagg
     1621 gtcgacgcgc aaacacaagt gatccatgtt ccgccccgca cccggcacgc cgttttcggg
     1681 gtgatcgagc ttcgcgccga ccttcaacag gtcgatcagc gagcgccccg cgcgcaattg
     1741 cgtcaggccc agatcaggct gctccttttc gacgctgcaa ccaagcacgt cgcaatagaa
     1801 gcgcgtcatg tccggaacgt tcgacgcccg aatgacgatg tgatcgatct cgcgaatgtg
     1861 gattctcatt tccaacgccc cttgcccgat tcgcagaatg gatgaataga caccagtgta
     1921 gaagaaagga aggcgcggcg agagcggcct tcaccagcct cgagggacgg atcagaaacc
     1981 tacagacgaa aaaaagcccg ctctatgtgg agcgggctga atccatatca ggaggagaca
     2041 tggaggagac aagtactaat atacacatgg ggttgatgcg acgcaataac tttttaaggg
     2101 aaaacccgat atcgggccaa aatgtcgcac tttgaggcga aacgagcgcc tggcgcggct
     2161 tccggcctac gaggaagcac aatgacagag caagaaccgg ggcgtagtgt gtcggcatgc
     2221 ggcctacatt ggtgagcatc gacattgcct ttccgggacc atcatgaacc gcactgaccg
     2281 tactgaaacc ggcgataccg gcagcgaaac cgctgtcggc cccagccgct ggagcaccga
     2341 cgccgaccgt tgggaagccg tcacccgccg cgagccgcag gccgacggcg cgttcttcta
     2401 cgccgtcaaa acgacggggg tgttctgtcg gccgtcgtgc gcgtcgcgcc agccgcgccg
     2461 cgaaaacgtc gctttcttta ccgacgccaa cgccgcgcgc gccgcgggct atcgggactg
     2521 caagcgctgc cagcccggcg gcctgccgcg cgaactggag atcgtcaatc gcgcttgcgc
     2581 cgcgctggat gccgatcctc agcaacgcct cacgctcgcg caattgagcg atgcggtgca
     2641 tgtcagcccg ttccacttgc agcgtctgtt caagcgcgtg gtgggtgtgt cgccgcgtca
     2701 gtatcaggcc gcgcagcgcg gcgcagcgtt gcgcgatgcg ctacagagcg ggtccgacgt
     2761 gacgcgcgcc acgctcgatg ccggtttcgg ctcgccgtcg cggatgtacg acagcgcctc
     2821 ggcggaactc ggcatggcgc cgtccgcgtt tcgccgcaag ggcgcggggc tcaccgtgcg
     2881 ctatgccagc gcgtcgactt cgctgggttt cgtgctggta gccgctaccg acaagggcat
     2941 ctgcaaaatc ggtttcggcg acgatgctgc gatactcgcc gacgaactgc gcggcgagtt
     3001 cgccaacgcc gatctggtcg aagacgcaac acgcctcgcg ccgttcatcg cgcagatcga
     3061 tgcctatctg cgcgggactc gtcaggattt cgagctgccc ttggatatcg ccgccaccgc
     3121 gttccgccaa cgtgtctggg acgcgctgcg gcgcattccc tacggtgaga cgcgcagcta
     3181 ttcccagatc gcggaagcgg tcggcgcacc ccgcgccgtg cgcgcggtgg cgagcgcgtg
     3241 cgcgacgaat ccggtcgcgt tggcgattcc ctgccatcgc gtcgtgcaga agggcggggc
     3301 gctggcggga tatcgctggg gtctgccgcg caaggccgcg ctgctcgaca acgaggccca
     3361 gcatgcaggc gatgtttcgc ttgctgaacg tgaccgccgc gagcgcgccg ccctgactgc
     3421 aaccaccacc aacctggacc acgccgcgtg agcacgcttg acgacgtcgc aacactcgaa
     3481 ctgccattca aagcaccctt cgactggctc cgcctgctgc gttttttcgg cggacgcgcg
     3541 acgcccggcg tcgaaaccgt cgaagacggc gcctaccgcc gcgcgatcga ctggaccggc
     3601 gacagcggca cgctgaccgt gcgcctgcat ccgcgcaagc gctgcatcgt cgcgaacatc
     3661 gacggtcccg cgagccgtca tgccgatgcg ctcgccgcgc cgatcgcgag gatgttcgac
     3721 ctgcacgccg acccgaagaa gatcggtggc gaacttgccg ccgatccatg gctcgcgccg
     3781 ttgatcgagg ccgtgccagg cttgcgggtg ccgggcgcgt ggtcgggctt cgagctggtg
     3841 gtgcgcgcga ttgtcgggca gcaggtcagc gtgaaggcgg cgaccaccat catcgggcga
     3901 ttggtgcagc gtgcgggcga acgcatcgaa ggtcatccgc atgagaacac cgcctggcgc
     3961 tttccaacgc ccgccgcgct cgcggctgtg gatctggaca agatcggcat gccgggtaaa
     4021 cgcgtagcgg cgttgcaagg ctttgctcag gcggtcgctt caggcgacgt gccgctcgac
     4081 agcggcgtcg ccgacgccga cggtttgcgt gcggccttgc tcgcgctgcc gggcatcggt
     4141 ccatggacgg tggaatatgt ggcgatgcgg gcatggcgcg acgccgatgc gtggcccgca
     4201 tgggacctcg tgctgatgca gtcgatctgc gcgcgcgacc cgtcgctcgt gcggcctacg
     4261 cagcaacgca cccgcaccga cgcctggcgg ccgtggcgcg cctatgcggc catgcatttg
     4321 tggaacgagg tggcggaccg ggcgggtgcg gcgcgcggcg gctgaggtcg tgtggcaatc
     4381 tgcgcgcgcg acccatcgct cgtgcggcct gcagccgcac cgacgcctgg cggccgtggc
     4441 gcgcctatgc ggccatgcat gtgtggaacg aggtcgtgga ccgggcgggt gcggcgcgcg
     4501 gcgcggctga ggtcgtgtgg cagtcaggaa gcgagactgg cgagcggggg cggcgcccca
     4561 gctcggcggt tgcggtcgta tggcacacag cgagccccca caagttgtcg gactggaacc
     4621 ggcgcctcca cgccgcggct gaggtcgtaa gtcgtgcggg gacatacgcg aatcgcgcat
     4681 cgggcgtacg gcggatagtg tgacgaggcg cagggctggc cctcttttgt cctgaaaata
     4741 ggcggaatga agcggcggag ggcccgccga agcggcctca agcagccgcc gtggcgagat
     4801 gttaaagtgc ccgctacccg aaaattccgc cgaacgttgt caaccgcgca gtgctgcatg
     4861 gagcagcgaa gcggcacatg gcggccgaca acgcccgact cgcatcaatg ccaaacacca
     4921 caccttcccg cacgaccgac gcgttttcgc accgcctgtc cgtgctgact tcaggcccgc
     4981 agcgcgatgc gttgatacgc ggcctgcgcg gcatcgaaag ggaaagcctg cgcgtgacgc
     5041 acgacggcca gctggccatg acgccgcatc cgcgcgcgct cggttcggcg cttacgcatc
     5101 cgtcgctcac caccgactat tccgaagcgc tcctcgaatt gatcacgccg gctgagcacg
     5161 acgtcgcgat cacgcttgaa aatctggata cgctgcatcg tttcgtctac gccgaactcg
     5221 gcgacgagat cttgtggaac aactccatgc cgggcctgct gcccgaaacg gacgacggca
     5281 ttccgatcgc gcattacggc acgtcgaaca tcggcaaact gaagtacgtg taccgcattg
     5341 gcctggcgct ccgttacggc cgcacgatgc agtgcattgc gggcatccac tacaactatt
     5401 cgctgaacga agaagtgtgg cgggtgttgc atgccgacca gcaatccacg gcaaacgccg
     5461 tcgatttcca gtccgaccgt tatctcgcgc tgatccgcaa tttccgccgc accaactggc
     5521 tgctgatgta cctgttcggc gcgtcgccgg cactggatcg ccgattcctc cgcgatcgcg
     5581 aacacacgct cgaaacgctc gacgccgaca cgctgtatcg cccgtacgcg accagcttgc
     5641 gcatgagcga tctcggctac tcgaacacca ccgcgcaagc cgcactgcat gccgactacg
     5701 acacgctgcc gggctacctc gacgcgctcg cgaaggccgt gagccagccg tatcccgcgt
     5761 atgaggcgat cggcacacag cgcgacggcg agtgggtgca gatcaacacg aacgtgctgc
     5821 agatcgaaaa cgagttctac tcgacgatcc gtccgaagcg catcacgcat ccgggcgagc
     5881 gtccgctgca tgcgttggct gcgcgtggcg tgcaatatgt cgaagtgcgt tgcatggaca
     5941 tcgacccatt cgagccgacc ggcatctcgc tggagacatc gcgttttctc gacgcttatc
     6001 tgctggtctg cgcgctcgac gaaagcgcgc cgctgccgcc gcacgcgtac gcggaggcga
     6061 atcagaactt cggccgcgtg acgatggaag gccgcaagcc gggcctcgaa ctgacacgcg
     6121 acggcagccc gattgcgatg acggattggg cgaacgaact gctcgccaag atcgacgcag
     6181 ccgccgcaac gctcgacgcc ttgcacggcg gcgacgcgca cgcacgcgcc gtggcggttc
     6241 agcgcacgaa gctggcggac gcatcgctga cgccgtccgc gcgcgtattg cagacgatgc
     6301 gcgacaagca gcagagcttc ctcgcgtttg ctcttgaaca aagtgaagtg cacgccgcct
     6361 atttccgttc gcgtccgctg gatgctgcgc agacgaaaga attcaccgac cttgcagcgc
     6421 aatcgcttgc cgagcaggcc aaacttgaac gggaagaggt gggttcgttc gacgcatttg
     6481 tcgcggcata ccgggcatac acgctgaacc ggtttagcgt ctgaacgacg cggcagacca
     6541 taggccaata ctgtttggct gtcgcagaag ggcacaactg ccgaccaacc ccacgttttt
     6601 cggagcgtgg ggttttgttt ttcagccgcg gcgaatcact gtgcggagca cgatctagag
     6661 ggccagcaag gccagtctca tatgtagagc gaccgacgca gactgccgac caaccccacg
     6721 tgtttcggag cgtggggttt tgttttcagt cgcggcgaat cgcttgtgcg gaacgcgagg
     6781 ttgagggcca gcaaggccag tctcatatgt ggagcgaccg acgcagactc ccgaccaacc
     6841 ccacgtgctt cggagcgtgg ggttttgttt tcaggcgcgg cgaatcgctg tgcgaagcaa
     6901 gcgtttgagg ggccagcagg gccagcctca atgtgtagcg ggaagtggtc ttcgggctat
     6961 gtctttgcat aggtaacgtc tacccgaagg ggagcgtgat gtctggcgac gccggcaaaa
     7021 tctccccttg tttggtgatt cgttgctgcg tatgaaacac cgtgccgtgg ctgcggtcca
     7081 acataaatgg cacgctcgag agagggaggc ttcaccgcga tgaaaaaaga tctgttggcc
     7141 gtactgtgcg tagcggcggt gtcggcggcg ggatgttcaa cgcaggggca ggtgaacccg
     7201 gacgtcatgc agatcgcgac aacgccgttg acgtgttcga acaaggccga gtgtgatatt
     7261 tggtggcaac gcgcgcagac gtgggtaacg agccattcga agtacaaggt tgaatccgcg
     7321 agtgacacgc tgatccagac agcggggccg gacggcggca agcgcgcgct ggcgtaccag
     7381 atcacgcgca cagcgaaccc ggacggcacg gcaacgattg gctttgccgc gcattgcgac
     7441 ggctcgctgg gctgcaaacc gaacccatgg gaagcgggcg cggacttcaa ggaatacgta
     7501 cgaggcgcag gcgcaccgca gagcggcgca aacccaaccc aacctgcgcc gacggcgatg
     7561 catccggacg ccgcaggaca atcgatcccg ggaacgagtg gggaagcgat aacgcagtga
     7621 gtgggaattg atacgttgca gcgatcctct ctataggcat ccggccgtct gtcccccggc
     7681 ggattcgagc tttccgagtg cacgcgcccg caaactcaaa atgttatctt tgtagaccaa
     7741 tcgccgagcg ttgaattcct gcgcaatatt ctcttccgtt tccgagatcc cgggcgcttt
     7801 ggcgttgggt agataggaca cttcgacttc ggcggcgtag cctgttgcgg gtaattgttg
     7861 tttgagaacg ttgccacacg aatcgcaagg tgcctgggaa atgtatccga ccaatctgcc
     7921 accgcgtggc aatccattat gaaggatgtc gttttcaaag ttcttcagag ccttgagttc
     7981 agcatcggcg gcctgcttgt ctatattggc ggccaaccgc ccttcgggaa cttcaatgtt
     8041 acgtgccgct ttctctacac ggcccccctg tagaaaagcg ttcaattggc gatgtggggc
     8101 actccctgcg tcgtacaaaa tatcctgtgg atcctggccg gaaatagcat tgtagacacg
     8161 agtattactt tccccattgg ctccgccgta ggtgtattcg aagcgagcga tggtggcact
     8221 acgccacttg ctggccagaa gcttgatttt cgttgatgat ggtggggcag ttttgaccga
     8281 attgctcact gcttgcgcgt aaaattccat tgaattaagc aattgttccg gcacaagact
     8341 ggtagaactg ctcacctggc cgatgtttac ggggatttgc gcctgcccga ttgtcgaaaa
     8401 tgccagcagt aaagtggcgg cgcccaatct aaataggttt tcgaatttat tcatgataaa
     8461 actcctgtca ggttcagcgt catgccgggc aaaatcgatc gagataacga gcttcggacg
     8521 cacgaacctc ctcttctgtg aatgcttctt ccgcaccaag ctcagtcgcc tccaacgccg
     8581 cgaaaaattc caccgccgcc aagccgtcca ttatcatcgc gatcgggagt agtattctgg
     8641 ccagtttctc catggatgaa cccgaatcta tgggtctata aggtgacatt tccgccgcgt
     8701 atcgattctt gtaccaggaa taaagcgcct tgtccgtcat atctgtgaaa cacaaattgc
     8761 cggagttctc cggattctgg ctttttccgt cgatagaaca tggaggatga cctatataac
     8821 cttgcgcgtc ctgataagca gctgctaacc gtgcgatgcg aaactgggcg ggaggagtga
     8881 attcgccata cttgaatttt tcggattgat agtcgcttga tcgcaattga cgatgaagca
     8941 gattcatcaa ataattcttg tcgtattcgg agatgttatg gacattggtt acagccgtgc
     9001 tcaggtaacg atccagaaca tccttacgga taccattcct gctttgctcg gcaggcagga
     9061 cttgccgtgc cgagtcgcgc agaatttgcg ctgcaacggc gaatttggcg gcgatcgtaa
     9121 tatatttcga tccaaaaata aaaatgcttt tataaaaaat atcgccatca actccggctt
     9181 tggtcgcatt cgcaacgacc agattgcttt cttccgcacg gtaacccggg cggcggcgac
     9241 tcatattgat ctcgatgtca ggtcgtgcct tggttagccc gttttcgttt gcaaatagct
     9301 taaccgagtc ggcgagtgtc agactcactc gattgtcatg attaaagtca tcaagcgcca
     9361 gattccattc catcgtattg atggcagttg gttgactttt catttgagaa tacactgaat
     9421 caggggctac caggaagtcc tgatcgacat tagcgttggt aatagactct ccgagtccac
     9481 tttttactcc gtgataaata actgattttg taagcgtatc agcgaagaat ttttgctggg
     9541 ccaccgcggg ctgtaccgat gtaagtgtga gacaactcca ggccaaccct atagtcttaa
     9601 ccagtctgaa aaactgattc gatgaggcaa gcgggcgaga gttcatgcga agtgttttca
     9661 tcgcggcagt cctgtaacac ctgattaaaa tacgcaagac aaagttcgac ctacctgact
     9721 attaagtcgt gagatctccc ggccttactg ctttaaaaca gcataatttc aatgttcaga
     9781 atgaaacaga ggcaaattcg cacattcggg tttccccgaa tttttgcatt aataatgaga
     9841 taagatttta ttaaagtgga gggttgacgt taacaagctc gcctgacgga ccgaagctgg
     9901 agtcaagggc gccgcccgca ttggggtagc gccaataacc cagtaagaag ctccgcgcaa
     9961 caaagaattt taatgcccca tcgacgcccc aacccccttc cgcggcttag taacccaaac
    10021 aagcccagcc aacacaagaa acgccgcaca agaaatgcga aaaaaatcat tagtggccat
    10081 catataagcc tgcgccgtaa cgacctgatt caactgagcg gtgacaccat caccagaaaa
    10141 cccaagagcc gccagcgcac tggtataagc attggtacca gccgcataaa cattaaccga
    10201 atcggtcagc atcgcatgat gataaatcgt atcgttttcc cagtaagtcg tactgatagc
    10261 cgtcccaatc gcccctgaca aagtccgaaa aaagttagac aaccccgaag cactagccag
    10321 ccgctcatcc gacacgctgg acagcgtgat agtagtcatc ggcacgaaga agcaagcgac
    10381 accaatgcct tgcaccagcc gtggccagat cacatgatta aacggtacat caagcgtaaa
    10441 cgtagaattc cagaacgaca caaacgcaaa cacgataaaa gcaaaactcg ccacaacgcg
    10501 cagattcagc cgatgcatgt tcttgccgat cagcggcgac aaaaacagcg cgagaatccc
    10561 gaccggcgcc gtcgccaaac cggcgatgcc cgcggtgtaa cccatcaccg tctgcagcca
    10621 gagcgggaaa atcaccaccg agccgaagaa cgccataaac ccgaacgaaa tgatcagcac
    10681 gccgagtgcg aagttacgat ccttgaatag cgagagatcg acaacgggtt ccttgtccgt
    10741 catctcccac accagcagga acgcaatcga taccactgcg atgatcgtca gcgtcacgat
    10801 aaaggtcgag ttgaaccagt cgcgatcctt gccgagatcg agcaccatct gcagacatga
    10861 cacgccgatc acgagcaagg tcaggccgat cgcatcgata cgctgtttgg tgaccttggt
    10921 ctcgcggccg cgcagcagca gaaacgcgca caccgcggag aacaagccaa tcggcacgtt
    10981 gatgtagaag atccacggcc acgtgtagtt gtcggtgata tagccgccca tcacgggtcc
    11041 gaaaatcggc gcgcaaatca cggtcatcgc ccacaggccg agcgcgagcc cgcgcttttc
    11101 cggtgggtat gagcgcatca gaatcgtctg ggagagcggg accatcggcc cggagacgag
    11161 tccttgcaga agccggaacg cgatcagcga ctcgaagttg tgcgcgaagc cacatagcgc
    11221 cgaggcgatc gtgaaaagca gaacagacag cgtgaacagc cgcacttcgc cgacgcgccg
    11281 cgcgagccag ccggtcagcg gcaccgcaat cgccgaagct acggaatacg acgagatgac
    11341 ccaggtgccc tgactggtcg ccacgccgag gctaccggaa atggtcggca cggcgacatt
    11401 cgcgatcgat gtgtcgagca cttccatgaa cgtgccgagc gcgaggccga cggtgagcaa
    11461 cgccagcgtc ccgcccttga gcggggcggg ttgggcggcg ggtagagcgt cgacgggagc
    11521 cgtggcagcc atgtgtttat ccttagccgg gaaagcttgt cgcggcagct ttgtcgcacc
    11581 ctctaagcgg cgcggttaca tgccggatca ccctctgccc agacagatat tttcagacaa
    11641 tggcgctttc aaaagcgcct cttggttatt gcgcgccgag ctctgccggc ggttcttcgg
    11701 gtgatgcttc ctgcgattcc tgcggcgctc cgagcccgca atctgcgctt gcgccgccgc
    11761 cgatgccatt cgcaatgaaa cggcccagca ggtcgagcag cgtggcatgg tccgcggcgg
    11821 aaaatccgcg caactgctcg ttcagcacgg cagcgccaag agcgggtaac tgctgcgcgg
    11881 cttcgcggcc ttgctgcgtc aactccagct tgaccatccg acggtcggca tcactccgcg
    11941 agcgcaccac aaagcctttc ttttcaaggc gatcgagcag gcgcgtcatc gatccactgt
    12001 cgtacgacat gacgcgcgac aactcgaacg gcgtgctcgc gcgttgcgcg gacagcagca
    12061 gaatcacacc aatctgctgg gcggtcagcc ctaacgggtt gaccgcacga tccatccgct
    12121 cgaccagcac gtttcgcgct ttcgtcaggt aatagccaag actcgattca aggtgaatct
    12181 cgtccgcgtt gtaggggccg tcggtcatcg ggtttccggg aacatcgtca agcagaacgg
    12241 attttagctg cctaagcaga taagtcaaat ttcatttcgc gtagggcgaa tgacgcaaaa
    12301 aacaacaccg tcattgcacg cgaagcagag tatcttgaaa tttaattgca tagtcaatat
    12361 tattgacggc aacgagttac gcgcgacccg cgccacccga gaccatgttc tgaccgattg
    12421 tctgcacgcc gcaatggcgc cgtgcgaaaa aggatttccc ccatgctgta cctcaaaaac
    12481 accctcggcg gcttgagccg ctccctgacc gattccgcgc tgagcagctt ccaaggtccg
    12541 catcgctggt ggaaactggc gttactcacc gtgctgttcg tgctgccggg tggatcggtg
    12601 ggtgtcgcgc tgtttgcgtg ggtggaacat cggcgcgggc ggaagggcgc tcaggctgca
    12661 ccggaagaat cggtgggatc cgccgttgcg gcgacaagtg ccgttgcggc tacgaccgct
    12721 gcgacgcgtt cctcaagcgc ttgtcatggg cgcttatgtg cgccgtgtcg ggctgcggcc
    12781 ggaaagctag cccggcagac cacgtcagcg gattcgcggg tttaattcct ttctcgcaga
    12841 gccggctttc gttgcccgcg cggcgttttt gacgtcgcca aggataacga cgccggtaat
    12901 agcatgtaac catgccgaca ccctaaataa cacacctccg cctcccccag cattaccgtt
    12961 tcagtttccc gcgcccaagc agctccatac gctaacattc cggcagagcc atgatctcgc
    13021 ccgcccggga gtgccgccgt cggccgcact gcggcgtcca gaaggaatcc attgcatgca
    13081 gtttgatcga atctcgcgcg cgcgggcgct tgcgccccgc gccctgacta tcgccgtagc
    13141 cgctgccctc ggcaccctgc tggccggatg cgcagtcggc cctgactaca agcggcccgc
    13201 agccgaaatc cccgcttcct acaaggaagc cgcccccggc tggaaagtcg cccagccggc
    13261 cgaccagcaa gaccgcggtg actggtggac cctctacgaa gacccgcaac tcaatacgct
    13321 cgaagacaag ctgaacacgt cgaaccagac catcgcccag tttgccgccg cctatcgcca
    13381 ggcacgtgcg ctggttagcg aggcacgctc ggcgtatttc ccgaccgtcg gtgcatcgat
    13441 aggcgccacg cgctccggca acggctcatc gtccaatggc actcccactt cggtgtcgag
    13501 ccgcagcggc gtcagcaaca gctacaacgt gcaactcgac gccagctggg agccggatct
    13561 gtggggctcg gtgacacgct cggtgaacgc gcagaaagcc gg
//
LOCUS       DATASH010000149         1971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6944425,
            whole genome shotgun sequence.
ACCESSION   DATASH010000149 DATASH010000000
VERSION     DATASH010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6944425"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1132
                     /locus_tag="VHV99_04480"
     CDS             <1..1132
                     /locus_tag="VHV99_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEX3634732.1"
                     /translation="QVIDVWQDCRSLRIIITREEGSWRPRFRHWRLGGTERFFDRGIM
                     LFSATSAAAAVIIACTLWIGSGWADGASAVTLAAVACCFFAALDEPAPMVFRFFVATA
                     IAVVAAGLYLFVVLPHVHEFAMLVILFAAPFILVGTLMPRPQFNLVTVLVAVNTATFI
                     SIQEAYSADFLIFMNSNLAGLAGLLYAYLWTRVTRPFGAELAASRLLRSSWADVALTA
                     STRTIDDPRNLAARMLDRLMQLIPRLAATDDHRHPSIESFRDLRIAFNALDLRRLTAR
                     LGGDAPAAIAHVLEDVQQYFESCIDLRRRQPVPQSLMSSIDAAVARVTAQGVANADAP
                     SATSATSARQLRDALHALVGLRLSLFPATLIIPTPPVPEAAA"
     gene            1178..1387
                     /locus_tag="VHV99_04485"
     CDS             1178..1387
                     /locus_tag="VHV99_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HEX3634733.1"
                     /translation="MIGEIDIFGVFVPAVLVLMLIAYLINLGIRTVLARVGFYRFVWH
                     RSIFDLGIYVLVLGLVVVVSHRLIT"
     gene            1384..>1971
                     /locus_tag="VHV99_04490"
     CDS             1384..>1971
                     /locus_tag="VHV99_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX3634734.1"
                     /translation="MKKTWFSVGQILLTLIVVVVAAFVLWKLVAYYMFAPWTRDGHVR
                     ADVIQVAPDVSGLISSVDVVDNQQVRQGQVLFVIDQARYALALRQAQATAQQRRATLD
                     QARREDARNRQLGNLVAAEVAEESRSRVDQAEAALADANVAIDTAKLNLQRATIVSPV
                     DGYLNDRAPRVGEFVAAGRAVVSVVDMHSFRVDGYF"
BASE COUNT          281 a          665 c          614 g          411 t
ORIGIN      
        1 acaggtgatc gacgtctggc aggactgccg ctcgctgcgc atcatcatca cgcgcgaaga
       61 aggctcgtgg cgcccccgct ttcgccattg gcggctgggt ggcaccgagc gcttcttcga
      121 ccgcggcatc atgctgtttt cggcgacttc cgcggctgcc gcggtgatca ttgcgtgcac
      181 cctgtggatc ggttccggct gggcggacgg cgccagtgcg gtgacgctcg cggcggtggc
      241 ctgctgcttc ttcgccgcgc tcgacgagcc cgcgcccatg gtgttcaggt tcttcgtcgc
      301 cacggcgatc gccgtagtgg ccgccggcct ctatctgttc gtcgtgctgc cgcacgtgca
      361 tgaattcgcc atgctggtca tcctgttcgc cgcgccgttc attctggtcg gcaccttgat
      421 gccgcgcccg cagttcaacc tggtcacggt gctggtggcc gtcaacaccg ccaccttcat
      481 cagtattcag gaggcctact cggccgactt cctgatcttc atgaacagca atctcgcggg
      541 cctcgccggc ttgctctatg cctatctgtg gactcgcgtg acgcgaccgt tcggtgccga
      601 acttgcggcc tcgcggctgt tgcgctcgag ttgggccgac gtggcgctga ccgcctccac
      661 ccgcacgatc gacgacccgc gcaatctcgc cgcgcgcatg ctcgaccgcc tgatgcagct
      721 gatcccgcgt cttgccgcca cggacgacca tcgccatccg tcgatcgaaa gcttccgcga
      781 tctgcgcatt gctttcaatg cgctggattt gcgccgcctg accgccaggc tcggcggcga
      841 tgcgccggcc gcgatcgcgc atgtgctcga agacgtgcag caatacttcg aatcgtgtat
      901 cgatctgcgg cggcgccagc cggtgccgca aagcttgatg tcgtcgatcg atgcagccgt
      961 ggcgcgcgtc acggcccaag gggtagccaa cgccgacgca ccgagcgcga cctcggcaac
     1021 ctcggcgcgc caattgcgcg acgcgctgca tgcactggtc ggcttgcgtc tttcgctgtt
     1081 tccggccacg ctgatcatac ccacgccgcc cgtgccggaa gccgctgcct gatgcgcccg
     1141 tcccgccatc ccatccatcc ctttgcccat ttccgcgatg atcggtgaaa tcgatatctt
     1201 cggcgtgttc gtgccggccg tgctcgtgct gatgttgatc gcctatctga tcaacctggg
     1261 catccgcacg gtgctcgcac gcgtcggctt ttaccgtttc gtctggcacc gttccatttt
     1321 cgatctcggc atctatgtgc tggtgctggg ccttgtcgtt gtcgtttcgc acagactaat
     1381 aacgtgaaaa aaacctggtt ctccgtcggt caaattctgc tgaccctgat cgtcgttgtg
     1441 gtggcagcct tcgtgctgtg gaaactggtc gcctattaca tgttcgcgcc gtggacccgc
     1501 gacggccacg tgcgcgccga cgtgatccag gtcgcgccgg atgtctcggg gctgatttca
     1561 tcggtcgatg tggtggataa ccagcaggtc aggcaaggcc aggtgctgtt cgtcatcgat
     1621 caggcgcgtt atgcgctcgc gctgcgtcag gcgcaggcca ccgcgcagca gcgccgcgcc
     1681 acgctcgatc aggcgcgccg cgaagatgcg cgcaatcgcc agctcggcaa cctggtcgct
     1741 gccgaagtcg ccgaagaaag ccgctcgcgc gtcgaccagg cggaagccgc gctggcggat
     1801 gcgaacgtcg cgatcgacac cgcgaagctg aacctgcaac gcgcgacgat cgtcagcccg
     1861 gtcgacggtt atctgaacga ccgcgcgccg cgcgtcggcg aattcgtcgc ggcggggcgc
     1921 gcggtggtgt cggtggtgga catgcattcg ttccgcgtcg acggctattt c
//
LOCUS       DATASH010000150         4872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1406131,
            whole genome shotgun sequence.
ACCESSION   DATASH010000150 DATASH010000000
VERSION     DATASH010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4872
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1406131"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1548
                     /locus_tag="VHV99_04495"
     CDS             <1..1548
                     /locus_tag="VHV99_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3634735.1"
                     /translation="LRYASVIGLSLVGGGVLNAPRARAQGAPSPSNQTIRVAHLTPAG
                     AVDPLTVTDAASLALLNQTGEFLIDDDGEKLVLKPALALSWKPNDKGDVWTFKLRTNV
                     KFHDGQAFAAKDVVATFDRLADPASGSAALSVLKGVLSKGGAKVVDDHTVAFHLDAPN
                     GNFPYYVSSDNYNAVILPANYAGGYEKSFIGTGPFKLEKYQAKVGASFVRNPDYWGDK
                     ALPQRVQFSFYADEQAQLLALQGHQADVMGTFTVQGGAGILNNPDYKAVGVKSSAHRQ
                     IHMRNDNPLFKDKRVRQALALSLDRDVLVRGLFKGRAQLGNDSPFAPVFPSSDAGVAQ
                     RKIDIAKAKQLLAQAGVPNGFDVTLTTEKYMEIPDLAVVVQNAAKAIGVRVNLKVESQ
                     SLYYGAGTPGKSDWLDSPLGITDYGHRGVPNVFLNAPLTSTGTWNAAHFKNPQYDQLV
                     AQFVAAIDLSSQKKVSGQIQTLLLDETPVIIPFFYDQLIAMRKGVNGVRFTALAQLYF
                     DRAVLSA"
     gene            1624..2634
                     /locus_tag="VHV99_04500"
     CDS             1624..2634
                     /locus_tag="VHV99_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3634736.1"
                     /translation="MPTTVSPSASPVSSGNAGRVVRFLATRVGLSLITLWLLSVIVFA
                     GGQLLPGDIGRAVLGPLADARAVAALNHQLGADRPLMTQYVAWISHFVRGDMGLSYAY
                     REPVGPFIADALAHSAKLGVLAFIVVVPLGIAGGVWSAMHAGRWLDRTISIAGLSATV
                     VPEFVSSIVLILVFGVWLRWLPIEASYPPEASALEQLRHLILPVLPLVLVFFGYIARM
                     ARAGTVEALDADYTRTAILKGLPRRTVIWRHVLRNALLPTITVAATQLGYMIGGLVVV
                     ETLFHYQGIGSLIYNAAKAKDFPMLEAGVLTIGVVYTVANLIADALHVLLNPRLRVRS
                     AE"
     gene            2631..3509
                     /locus_tag="VHV99_04505"
     CDS             2631..3509
                     /locus_tag="VHV99_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3634737.1"
                     /translation="MSTIVPPATPPAARAVTEPRFDQLRVLLRSPTFVVGVLIVAWWI
                     VCAIAGQWIVRIDPYASDPLNSLMPPDSTHWFGTDQLGRDVCSRVIVGARDILTIAPL
                     ATLLGTVAGTGLGLIVGYFEGWVDNVVGRAIDAVLALPLVIVALLALAAVGASNLTVI
                     LVIGITFTPITARTVRAAVFAERHLDYVAAAQLRGERAPYIMFAEILPNVLPPIIVEA
                     TVRLGYAIFAVATLSFLGFGIQPPSADWGLALSESYTLMAGGAWWTVVFAAGAIASLV
                     VGVNLIADSVQGVLDR"
     gene            3506..>4872
                     /locus_tag="VHV99_04510"
     CDS             3506..>4872
                     /locus_tag="VHV99_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX3634738.1"
                     /translation="MNGPPPASFPAFDVSKSDRTDALTVVGLTVTYRMRGRDREVLQD
                     VSFRVRRGEAYGLVGESGCGKSTVAMAALRYLPRNGKVKAGKIIIAGQDVQKLDAHAL
                     RSMRANAISMVYQDPGRALNPSLTISRQVTEAFEAAGASRDEALRRTVEILQRVRIAS
                     PERVMESYPHQLSGGMQQRVVIAMALASNPALLILDEPTTGLDATVEAEVLDLVAQLR
                     EELGTAVLFISHNLAVIGRMCERVGVLYAGKLVEEGATQDVFARPRHPYTVGLLRCLP
                     GTGRSKDTERLDTIAGSLPAPGSVTQGCIYADRCRLADDRCRREAPPPHRVNAAHGDQ
                     MSRCHYHERAIDLPRASAEALSRHDDALRDRERSALVLRAEKLSKTFHISGAPLRAVD
                     NVSIDLAGGETLGLVGESGSGKTTLAKLMLGLLTPDAGSVLELDGTPLAARVTRRNDE
                     QIKSL"
BASE COUNT          813 a         1576 c         1573 g          910 t
ORIGIN      
        1 ctgcgctatg ccagcgtaat cggtttgtcg ctggttggcg gcggtgttct gaatgcgccg
       61 cgagcccgtg cgcaaggcgc gccgagtccg tctaaccaga cgatccgcgt ggcccatctg
      121 acgccggccg gcgcggtcga cccgctcacc gtgaccgatg ccgcgagcct cgcgctcttg
      181 aaccagactg gcgagttcct gatcgacgac gacggcgaaa agctcgtgct gaaaccggcg
      241 cttgccttgt catggaagcc gaacgacaag ggcgacgtgt ggaccttcaa actgcgcacc
      301 aacgtcaagt tccacgacgg ccaggcattc gccgccaagg acgtggtcgc caccttcgat
      361 cgcctcgccg acccggcgag cggctcggcg gcgctctcgg tgctcaaggg cgtgttgtcg
      421 aaaggcggcg cgaaggtggt cgacgaccac acggtcgcct tccatctcga tgcaccgaac
      481 ggcaacttcc cgtactacgt ttcctcggac aattacaacg ccgtgatcct gcccgcgaac
      541 tatgcgggtg gttatgaaaa gagctttatc ggcacgggtc cgttcaagct cgaaaagtat
      601 caggcgaagg tcggcgcgtc gtttgtgcgt aatcccgatt actggggcga caaagcgttg
      661 ccgcaacgcg tgcagttctc gttctacgcc gacgaacagg cgcagttgct cgccttgcaa
      721 ggtcaccagg ccgacgtgat gggcaccttc accgtgcaag gcggcgcagg cattctgaac
      781 aatccggact acaaggcagt cggcgtgaag tcgagcgcgc atcggcagat tcatatgcgc
      841 aacgacaatc cgttgttcaa ggacaagcgc gtgcgccagg cgttggcgtt gtcgctcgat
      901 cgcgacgtgt tggtgcgtgg cctgttcaag ggccgcgcgc agcttggcaa cgacagcccg
      961 ttcgcaccgg tatttccgtc atcggatgcg ggcgtcgcgc aacgcaagat cgacatcgcc
     1021 aaggcgaaac aactgctcgc gcaagcgggc gtgccgaacg gcttcgacgt cacgctgacc
     1081 accgagaagt acatggagat tcccgatctc gcggtggtcg tgcagaacgc ggcgaaggcg
     1141 atcggcgtac gcgtcaatct gaaagtggag agccagtcgc tgtactacgg cgcgggcacg
     1201 ccgggcaaat cggattggct cgattcgccg ctcggcatca ccgactatgg tcatcgcggc
     1261 gtgccgaacg tgttcctgaa tgcgccgctc accagcaccg gcacgtggaa cgccgcgcac
     1321 ttcaagaacc cgcaatacga tcaactggtc gcgcagttcg tcgcggcaat cgacctgagt
     1381 tcgcagaaga aagtctccgg acagatccag acgctgctgc tcgacgaaac gcccgtgatc
     1441 atcccgttct tctacgatca gttgatcgcc atgcgcaagg gcgtgaacgg tgtgcgcttc
     1501 accgcgctcg cgcaactcta tttcgatcgc gcggtgttga gcgcgtaatt tgaatgcaaa
     1561 gctgttgaac gcaaacgccc ttgaacgcat gagcgcacgg cacttctttg gcaggagatc
     1621 acgatgccga ccacggtgag cccttccgcc tctccggtct cgagcggcaa cgccggccgc
     1681 gtcgtacgat ttctggcgac gcgggtcggc ttgtcattga tcacgctttg gctgctgtcg
     1741 gtgatcgtgt tcgccggcgg gcaattgttg cctggcgata tcggccgcgc ggtactcggg
     1801 ccgctcgccg atgcgcgcgc ggtcgcggcg ctcaatcatc agcttggcgc ggaccgtccg
     1861 ctcatgacgc agtacgtcgc gtggatctcg catttcgtgc gcggcgatat ggggctgtcc
     1921 tatgcgtatc gcgaaccggt cgggccgttt atcgccgacg ctttggcgca ttcggccaaa
     1981 ctcggtgtgc tcgcgtttat cgtggtcgtg ccgctcggca ttgcgggcgg cgtgtggtcg
     2041 gccatgcacg cagggcgctg gctcgatcgc accatcagca tcgccggctt gtcggcaacc
     2101 gtggtgccgg agttcgtctc gtcgatcgtg ttgattctgg tgttcggcgt gtggctgcgt
     2161 tggctgccga tcgaagcgtc gtatccgccc gaagcgagcg cgctcgaaca actgcggcat
     2221 ctgattctgc cggtgctgcc gctggtgctg gtgttcttcg gctacatcgc gcggatggcg
     2281 cgcgcgggca ccgtcgaagc actcgatgcg gactacacgc gcaccgcgat cctcaaaggc
     2341 ttgccgcgcc gcaccgtgat ctggcggcac gtgttgcgca atgcgctgct gccgaccatc
     2401 accgtcgccg cgacgcagct cggctatatg atcggcggtc tcgtggtggt ggagacgctg
     2461 ttccactatc aagggatcgg ttcgctgatc tacaacgccg ccaaggccaa ggattttccg
     2521 atgctcgaag cgggcgtgct gacgatcggc gtggtctaca ccgtcgccaa tctcatcgcc
     2581 gatgcgctgc atgttctact aaatccgcgg ctgcgcgtga ggagcgccga atgagcacca
     2641 tcgttccgcc ggccacgccg cctgccgcac gtgcggttac tgaaccgcgc ttcgaccagt
     2701 tgcgcgtgct gctgcgctcg cccacctttg tggtcggcgt gttgatcgtt gcatggtgga
     2761 tcgtctgcgc gatcgccggg caatggatcg tgcggatcga cccgtatgca tccgatccgc
     2821 tcaactcgct catgccgccc gacagcacgc actggttcgg caccgatcag ctcggccgcg
     2881 acgtgtgctc tcgggtgatc gtcggcgcgc gcgacattct gaccatcgcg cctttggcga
     2941 ccttgctggg cacggtcgcg ggaactggcc tcgggttgat cgtcggctac ttcgagggct
     3001 gggtcgacaa cgtagtgggc cgcgcgatcg acgccgtgct cgcgctgccg ctcgtgatcg
     3061 tcgcgttgct cgcgctggcc gcggtcggcg catcgaacct caccgtgatc ctggtgatcg
     3121 gtatcacctt cacgccgatc accgcgcgta cagtacgcgc cgccgtgttc gccgaacggc
     3181 atctggacta cgtcgctgcc gcgcaactgc gcggcgaacg cgcgccgtac atcatgttcg
     3241 cggagattct gccgaacgtg ctgccgccga tcatcgtcga agcgaccgtg cggctcgggt
     3301 atgcgatttt cgcggtcgcg acgctgtcgt ttctcggctt cggcatccag ccgccttccg
     3361 ccgactgggg gctcgcgctg tccgagtcgt acacgttgat ggcgggcggt gcgtggtgga
     3421 cggtcgtgtt cgcggccggc gccatcgcct cgctcgtggt cggcgtgaat ctgatcgccg
     3481 acagcgtgca aggagtgctc gaccgatgaa cggcccgccg cccgcctcgt tccccgcctt
     3541 cgatgtttca aagagcgatc gcaccgatgc gctgaccgtg gtcgggctga cggtcacgta
     3601 ccggatgcgc ggacgcgatc gcgaggtgct gcaggacgtc tcgttccgtg tgcggcgtgg
     3661 cgaagcctat ggcctcgtcg gcgaatcggg ttgcggcaaa tcgacggtcg cgatggcagc
     3721 gctgcgctat ctgccgcgca acggcaaagt gaaggccggc aagatcatca tcgccggtca
     3781 ggacgtgcag aaactcgatg cccacgcatt gcgctctatg cgcgcgaacg cgatttcaat
     3841 ggtctatcag gatcccgggc gcgcgttaaa tccttcgctg acgatctcgc ggcaagtcac
     3901 ggaagcattc gaagcagccg gcgcctcgcg tgacgaagcg ctgcggcgca cagtcgagat
     3961 attgcaacgc gtgcgcatcg cgtcacccga acgcgtgatg gagagctacc cgcatcagct
     4021 gtcgggcggc atgcagcagc gcgtggtcat tgcgatggcg ctcgcctcga accccgccct
     4081 gctgattctc gacgaaccga ccaccggact cgacgccacc gtcgaagccg aagtgctcga
     4141 cctcgtggca caactgcgcg aagaactcgg cacggccgtg ctgttcatca gccacaacct
     4201 cgcggtgatc gggcggatgt gcgagcgcgt cggcgtgttg tatgccggca agctggtcga
     4261 ggaaggcgcg acccaggacg tgttcgcgcg gccacgtcat ccctatacgg ttggactgct
     4321 gcgctgcttg ccgggcacgg ggcgcagcaa ggatacggag cggctcgaca ccattgcggg
     4381 cagtctgccc gcgccgggtt cggtcacgca aggctgcatt tacgcggacc gctgccgcct
     4441 cgccgacgac cgctgccgcc gtgaagcacc gccgccgcat cgggtcaacg ccgcgcacgg
     4501 cgatcagatg tcgcgctgcc attaccacga acgcgccatc gatctgccgc gcgcgagcgc
     4561 ggaagcactc tcgcggcacg acgatgcatt acgcgaccgc gagcgctctg ccctcgtgct
     4621 gcgcgcggag aaactctcga agacattcca catttccggc gccccgctgc gcgcagtgga
     4681 caacgtctcg atcgatctcg ccggcggcga aacgctcggc ctcgtcggcg aatccggcag
     4741 cggcaagacg acgctcgcga aactgatgct cgggctgctc acgccagacg cgggcagcgt
     4801 gctcgaactc gacggcacgc cactcgccgc gcgcgtcacg cgacgtaacg acgagcagat
     4861 caagtcgctg ca
//
LOCUS       DATASH010000151         4163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1999379,
            whole genome shotgun sequence.
ACCESSION   DATASH010000151 DATASH010000000
VERSION     DATASH010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4163
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1999379"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="VHV99_04515"
     CDS             <1..645
                     /locus_tag="VHV99_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX3634739.1"
                     /translation="RMEPLLSHGYVSAEDVDRARTAQRSTLAELSAAQLQAQQAAAAV
                     SGVDALVAQRAVVMAEIATAELNLEYATVRAPFDGRIVSLKTSTGQFASALKPVFTLV
                     DTRHWYVVANFRETELKGVRTGTPATVYLMSDTGQRFQGTVDSISYGVSSDEGGLALP
                     GGLPRIQRTLNWVHVAQRFPVKIRVDHPNPELFRVGTSAVAVLEPRRGDDGERH"
     gene            655..2763
                     /locus_tag="VHV99_04520"
     CDS             655..2763
                     /locus_tag="VHV99_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007733963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEX3634740.1"
                     /translation="MAAADYLPDTLRNAGDFLKHELAPFPGRLNVMLRCMLTSAIVIV
                     ASMALEVPELALSLLVVFYVTQSNVVLTRLVGLMFIVGSTLAIGLSILVLKFTFDYPL
                     LRIVIASLLFFGSVYLLRVLKIGVVFFIVAIVVIYVQSFVDQTDQADLLIRAVLWVWA
                     AVNYPIALTLVVNTLLLPAEPQLQLKAEIHRQLAVLDMRLMQLIEGGTSAAPITLAAV
                     QQGALTLQKLLRFTTMRDARFREHQAAQLACIATVSRLYRGVSELQPAWPGATAARLA
                     MLRELHANVRALDEAVMSGEPYRYVSTATHEERNAADTIPAAGELQRALRAYADVVAR
                     GEEPGKPPASEPMIAPDAWTNPAYTRFSLKTLLAVLVCYVFYNAVDWQGVHTVMLTCL
                     IVALPSLGASTQRALLRLTGAAVGSVLALFMVVFIVPHFDDVVSLLLITLPVVALGAW
                     ISAGSERIGYAGLQVMFTFSLAVLGEFGPTENLTEIRDRMVGILLGVCVATFVQMSFW
                     REGEGDVLRQKLATLLRAIAAQLRSPQAGVDQRDELPYAQRQLQVWAVLADCEATLSR
                     VALEPGWQEVEQAQLTLRAQTVLAQGREIMLAGDALRTTLAAQAGSWSPRTVDAVRST
                     QEQASAELNRYADELASNPPAARAPRRIGIAAQVADPVQPFDGSLIAAADELSRQLAG
                     LPDWRVETPVEALSSKAIRT"
     gene            2823..>4163
                     /locus_tag="VHV99_04525"
     CDS             2823..>4163
                     /locus_tag="VHV99_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MdtP family multidrug efflux transporter outer
                     membrane subunit"
                     /protein_id="HEX3634741.1"
                     /translation="MSFVLSGCALIHHNGKPYAEIAPEQINLADDIHLARDGWPAARW
                     WTRYGDAQLNSLIDQALANAPTMVIARTRVAQAKSDVELARAGSNLQVAALASLDREH
                     ISANGFLGPFAKDEPAAGLTGPWYTEGIVGLGASLDVDIWGKQRAQIAASLGVNNARL
                     AETSAVELEISTDVAQLYYGIQTNYQLIGLLNELHEVAAFAVQAHQARAVRGLEARTL
                     PEEARAQQLAIEQQIVSAQAQIKQYRESMRALVGAGPGGLALIKTMALPPSQAALPAT
                     LSYELLARRPDLQAMRWYVESSFDKIDAAKAAFYPSFDIKAFFGFDALHLADLFTHAS
                     QQINLIPGLYLPIFDGGRLNANLSGAREGSNLLIEQYNEAVLNAVRDVAQTGSRLQAL
                     DAQTGLQKQRIESIAFTRDSTEASYQRGLTSRLAALEARQPVIAEQVALLTLNAQ"
BASE COUNT          724 a         1273 c         1363 g          803 t
ORIGIN      
        1 cgcatggagc cgcttttgtc acacggttat gtgtcggcgg aagacgtcga tcgcgcgcgc
       61 accgcacaac gctccactct ggccgaactc agcgccgcgc aactgcaggc gcagcaggcg
      121 gcagcggcgg tgagcggcgt cgatgcgctg gtcgcgcaac gcgccgtcgt catggcggag
      181 atcgcgacgg ccgaactgaa cctggagtat gcaaccgtgc gcgctccctt cgacggacgc
      241 atcgtgtcct tgaagacatc gacggggcag ttcgcatcgg cgctcaaacc cgtcttcacg
      301 ttggtcgata cgcgccactg gtatgtggtc gcgaactttc gcgagacgga actcaaggga
      361 gttcgtacgg gcacccctgc cacggtgtac ctgatgagcg atacaggcca gcggttccag
      421 ggcacggtgg attcaatcag ctatggcgtc tcgtccgatg aaggcggcct tgcgttgcca
      481 ggcggcttgc cgcgcattca acgtacgctt aactgggtcc atgtggcgca gcgtttcccg
      541 gtgaagatcc gggtcgatca tccgaatccc gaactgtttc gcgtcggtac gtcagcggta
      601 gcggtgctgg aacccaggcg cggcgatgac ggcgagcgcc attgaggagc gcccatggcc
      661 gcagccgatt accttcccga cacactgcgc aatgccggcg atttcctgaa gcacgagctt
      721 gcgcctttcc ctgggcgtct caacgtgatg ttgcgttgca tgttgacgag cgccattgtg
      781 atcgtcgcgt cgatggcgct cgaagtgccg gagctcgcgc tgtcgctgct ggtcgtcttc
      841 tacgtcacgc agtcgaacgt ggtgctcacg cggctggtcg gcctgatgtt catcgttggg
      901 tcgacgctcg cgatcggcct gtcaatcctg gtgctgaagt tcacgttcga ctatccgttg
      961 ctgcgcatcg tgatcgccag tctgctgttc ttcggcagcg tgtacctgct tcgcgttctc
     1021 aagatcggcg tcgtgttctt catcgtcgcg attgtcgtga tctacgtgca gagcttcgtc
     1081 gaccagaccg atcaggccga cctgctgatt cgcgccgtgt tgtgggtctg ggccgccgtc
     1141 aactatccga tcgcgctgac gctcgtggtc aacacgttgc tgttgccggc cgaaccacaa
     1201 ttgcaactca aggcggaaat ccatcggcaa ctcgcggtac tcgacatgcg gctgatgcaa
     1261 ctgatcgagg gcggcacgag cgccgcgccg atcacgctgg cggccgtcca gcaaggcgcg
     1321 ctcacgttgc agaaactatt gcgattcacg acgatgcgtg atgcccgctt ccgcgagcat
     1381 caggcggcgc agcttgcgtg catcgcgacc gtgtcgcgtc tttatcgcgg agtgagtgaa
     1441 ttgcagcctg cgtggccggg cgcgacggct gcgcggctgg cgatgttgcg cgagttgcac
     1501 gcgaacgtcc gggcgctcga cgaagcggtg atgtcgggtg agccgtatcg ttacgtgagc
     1561 acggcgacac acgaagagcg caatgcagcc gatacgatcc ccgccgcagg cgaattgcag
     1621 cgagcgctgc gtgcgtacgc ggatgtcgtc gcacgcggcg aggaacccgg caagccgccc
     1681 gcgagcgagc cgatgatcgc acccgacgca tggaccaatc ccgcgtacac gcgcttttcg
     1741 ctgaagacat tgctcgctgt tctcgtctgc tacgtgttct acaacgccgt ggactggcag
     1801 ggcgttcata cggtcatgct gacctgcctg atcgtcgcgc tgccgagcct gggtgcatcg
     1861 acgcagcgcg cgttgctgcg gctgaccggg gccgctgtgg gcagcgtgct ggcgctgttc
     1921 atggtggtgt tcatcgttcc gcatttcgac gacgttgtca gcctgctgct gattacgttg
     1981 cctgtcgtcg cgctcggggc gtggatatcg gcgggttcgg agaggatcgg ctatgccggc
     2041 ctccaggtga tgttcacctt ctcgcttgca gtgctcggag agttcggccc gacagagaac
     2101 cttaccgaga tccgcgaccg gatggtgggc attctgctgg gcgtctgcgt ggcaaccttc
     2161 gtgcagatgt cgttctggcg tgaaggcgag ggcgacgtgc tgcggcaaaa gctcgccacc
     2221 ctgttgcgcg ccattgccgc gcagttgcgt tctccacagg cgggtgtcga ccagcgggac
     2281 gagttgccct atgcacagcg gcagttgcag gtgtgggccg tgttggccga ttgcgaggcg
     2341 acgctgtcgc gcgttgcgct cgagcccggg tggcaggaag tggagcaggc tcaattgaca
     2401 ctgcgcgcgc aaaccgtcct cgcgcagggg cgcgaaatca tgctcgccgg cgatgcgttg
     2461 cgcaccacgc tcgcggcgca agcgggatcg tggagcccgc gaacggtcga tgcagtgcgc
     2521 tcgacgcagg agcaggcgtc agccgaactc aaccgatacg cggatgaact tgcgtccaac
     2581 ccgcccgccg cccgtgcgcc gcgacgcatc gggatcgccg cgcaggttgc tgatccggtc
     2641 cagccattcg atgggtcgct cattgccgca gcggacgagc tgtcgcgcca gcttgcgggg
     2701 ttgccggact ggcgcgtcga gacacccgtc gaggcactct catcaaaggc catacgcaca
     2761 tgaatgcgcg atcgggtggt caggattggc gatggcgtcg atgcttctta tgtgtcgcat
     2821 ggatgtcatt cgtgctgtcg ggttgcgcac tgatccacca taacggcaag ccttatgcgg
     2881 agatcgcgcc cgagcaaatc aatctcgccg acgacatcca tctcgcgcgc gacggctggc
     2941 ccgctgcgcg ttggtggacg cgctatggcg atgcgcagct caactcgctc atcgatcagg
     3001 cgctcgccaa cgcaccaacg atggtcattg cacgcacgcg cgtcgcgcag gcgaagtccg
     3061 atgtcgagct tgccagagca ggatcgaacc ttcaggtggc cgcgctggcg tcgctcgatc
     3121 gcgagcatat ctcggcgaac ggctttctcg gaccgttcgc gaaggacgaa ccggctgcgg
     3181 ggctgacggg cccgtggtac accgagggca tcgtggggct tggcgcaagc ctggacgtcg
     3241 acatctgggg caagcaacgc gcccagattg ccgcgtcgct gggcgtgaac aatgcacggc
     3301 ttgccgagac gtccgcggtc gaactcgaaa tctcgacgga tgtcgcgcag ctttactatg
     3361 gtatccagac gaattaccag ctcattggtc tgctgaacga attgcatgag gtagcggcgt
     3421 tcgcggtgca agcacaccaa gcacgcgcgg tgcgtggcct tgaagcccgt actttgccag
     3481 aagaagcacg ggcgcagcaa ctggccatcg aacaacagat tgtgtcggcg caagcgcaga
     3541 taaagcagta tcgcgaatcg atgcgggcgc tggtcggcgc gggtcccggc ggcttggcct
     3601 tgatcaagac catggcgctg ccgccttcgc aggcggcctt gcccgcgacg ctttcatacg
     3661 aactgcttgc acggcgcccc gatctgcagg cgatgcggtg gtatgtagag tcgtcgttcg
     3721 acaagatcga cgcggcgaag gcggcgttct atccgagctt cgacataaag gcgttcttcg
     3781 gcttcgacgc actgcacctc gccgatctct tcacgcatgc aagccagcag atcaacctga
     3841 ttccgggcct gtacttgccg atcttcgacg gcggcaggct caatgcaaac ctgagcggcg
     3901 cacgcgaggg aagcaacctg ttgatcgagc agtacaacga agccgtcctc aatgcagtac
     3961 gcgatgtcgc gcagacgggt agccgtttgc aagcccttga cgctcagacc gggttgcaga
     4021 agcaacggat cgaatcgatt gcgttcacga gggacagcac cgaagcgtct tatcaacgcg
     4081 gattgacgag ccggctcgcg gcactcgaag cgcgccagcc cgtgatcgcg gaacaggttg
     4141 ctttgctcac gctcaacgct cag
//
LOCUS       DATASH010000152        11737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2395668,
            whole genome shotgun sequence.
ACCESSION   DATASH010000152 DATASH010000000
VERSION     DATASH010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 11737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11737
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2395668"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VHV99_04530"
     CDS             complement(<1..405)
                     /locus_tag="VHV99_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006756361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HEX3634742.1"
                     /translation="MEQSPLYIRVHPNDNVAIVVNDGGLGEGAVFPDGLALRERVPQG
                     HKVALADLAEGDEVIRYYVVIGYALKALPKGSWINEHVIRMPSLPGLEDLPIATIKAP
                     EMPPLEGFTFEGYRNADGSVGSRNILAITTTVQ"
     gene            620..1537
                     /gene="kdgD"
                     /locus_tag="VHV99_04535"
     CDS             620..1537
                     /gene="kdgD"
                     /locus_tag="VHV99_04535"
                     /EC_number="4.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047448 - 5-dehydro-4-deoxyglucarate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxyglucarate dehydratase"
                     /protein_id="HEX3634743.1"
                     /translation="MTTPQELKQIVSEGLLSFPVTDFDEQGDFRADTYAERLEWLAPY
                     GASALFVAGGTGEFFSLTHDDYSNVVRTAAEVCKGKVPILAGAGGPTRVAIAYAKEAE
                     RHGANGILLMPHYLTEACQEGIAAHAEEVCKSVPNMGVIIYNRANSKLNADMLEGLAE
                     RCPNLIGFKDGVGEIENMVTIRRRLGDRFSYLGGLPTAEVYAAAYKALGVPVYSSAVF
                     NFIPKTAMDFYRAIAADDHATVGKLIDEFFLPYLAIRNRRAGYAVSIVKAGAKLVGHS
                     AGPVRAPLTDLTEEEMARLDALIKTLGPQ"
     gene            1672..3024
                     /locus_tag="VHV99_04540"
     CDS             1672..3024
                     /locus_tag="VHV99_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3634744.1"
                     /translation="MEMSRTASPAHVAKRTKVRYAILLLIFLITTFNYADRATLSITG
                     SAMRAEFGLDAIRMGYIFSAFSWAYVLSQLPAGWLLDRFGARRVYAASISLWSLFTLL
                     QSSIGLLGSAGAAVAALFVLRFAMGAAESPAFPANAKVVASWFPTNERGTASAIFNAA
                     QYFAAVIFTPLMAWLTHAFGWHMVYLVMGVAGLLLALTWLKVMKNPADHPRVSRAELD
                     YIEQGGGVVSGHRQAARAEDIASARGWSLVRQLLGNRMLLGVYLAQYCINVLTYFFLT
                     WFPIYLVQARGMTILQAGLVASLPAICGFSGGVLGGILSDGMIRRGHSLTIARKVPIV
                     GGMLLSVCIIGCNYVSTDWIVVALMSLAFFGKGIGALGWAVVADTSPKEALGLSGAIF
                     NMFGNVAGIVTPIVIGYLVAKSGSFNGALVFVGLNALLTVFSYLVIVKDIKRVELRRS
                     "
     gene            3279..4022
                     /locus_tag="VHV99_04545"
     CDS             3279..4022
                     /locus_tag="VHV99_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEX3634745.1"
                     /translation="MATPLASAVPPRRARNLAEFVVSYVSEQIASNALKPGDKLPTES
                     QLMVTLSVSRTVIREAISRLQAGKIIETRHGIGSFVLEPPREKLGIDMVPATTLRDVL
                     SILELRISLETECAGLAAQRAKADDLARMRTALDAIEATRHNGLDSVDADLQFHISVA
                     RATGNRYFVDILTQMGSALIPRNRLDSAGIAHAEPDAYLSLVNLEHESILEAITRHDA
                     EGARAAMRMHLSNSRERLRRANEAVEANS"
     gene            complement(4240..5823)
                     /locus_tag="VHV99_04550"
     CDS             complement(4240..5823)
                     /locus_tag="VHV99_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase (NADP(+))"
                     /protein_id="HEX3634746.1"
                     /translation="MSITGEMLIGRKAVRGEEKPLRAFNPATGSDIAEPVFGSGSVAN
                     VELACELAQKAFDPYRALPLTVRAEFLERIADGITALGDALIQRAHEESGLPKARLEG
                     ERGRTTGQLKLFAQVVRAGQWLAATLDSPLPERKPLPRSDLRMQKIPLGPVAVFGASN
                     FPLAFSVAGGDTAAALAAGCPVVVKAHRAHLGTSEMVGRAIQQVARDMDLPEGVFSMI
                     VGAGNSVGEALVAHPAIKAVGFTGSRAGGTSLMRVAAARAEPIPVYAEMSSINPVFLL
                     SNALSQRGESIARGFVDSLVLGAGQFCTNPGLAIAIDSDALKGFVATASEALSGKPAQ
                     TMLTAGIHAAYEDGEGKLAATKGVETVAQGIGATGPTQARAALFVTDAQTFLATPALE
                     DEVFGPASTIVRCKDEQEMLAVAEHFDGQLTATVQMDSADLAAAKKLVPILERKAGRI
                     LVNGFPTGVEVSHAMVHGGPFPATSDSRATSVGTTSIERFLRPVCYQDFPADLLPEAL
                     TDSNPLDLWRRRDGEITRS"
     gene            6052..6852
                     /gene="garL"
                     /locus_tag="VHV99_04555"
     CDS             6052..6852
                     /gene="garL"
                     /locus_tag="VHV99_04555"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HEX3634747.1"
                     /translation="MTAVAPYQPLPNSFRRAVCGGETLIGCWVSLASPIATELLGVVG
                     FDWMLLDAEHAPNDVLTLIPQLMALKDSPSAPVVRPPANDSVFIKRLLDSGFSNFLVP
                     FVESAEDAARAVAATRYPPQGIRGVSVSHRGNRYGTVSDYFDIANDNVCVIVQIESRK
                     AVDNIDEILAVDGIDGVFVGPSDLAASYGHLGNLNHPEVQQAIARIFERAQAAGKPSG
                     ILAPVQADAERYLSMGCRVIAVCADMGLLKAGAQAVQKHFMQKQAAPK"
     gene            6864..7778
                     /locus_tag="VHV99_04560"
     CDS             6864..7778
                     /locus_tag="VHV99_04560"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0019580 - galactarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HEX3634748.1"
                     /translation="MTSPEDSMQKAGFIGLGIMGKPMAANLLKNGVALAAFTRSGVPD
                     DLTQAGALACDSPAAVAAQADVIFIMVPDTPDVERVLFGEQGLAGALRPGQTVVDMSS
                     ISPMATREFAARVRERGADYMDAPVSGGEVGAKAGSLTIMVGGETATFERVKPLFDMM
                     GKNVTLIGDVGAGQVCKVANQVIVAATIEAVGEALLLASKAGVDPARVREALMGGFAS
                     SRILEVHGERMTKRTFDPGFRIELHQKDLNLALSTAQALGVSLPNTATCQALFNACVA
                     HGGKAWDHSAMVRALEILANHEIGQQAD"
     gene            8084..10081
                     /locus_tag="VHV99_04565"
     CDS             8084..10081
                     /locus_tag="VHV99_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEX3634749.1"
                     /translation="MATSRIPGQGAPNSPKRRAFIVLAGTSTGATLLGGLSACGGSVN
                     PPATTPPATPPVVQDPIWGTSGAATQIIASLQNITQAMFPARDYVVTQYGAAPCGVIA
                     AANPYTGSSSPTSPGSAQTNAANAPDARPAFLAAIQACNAAGGGRVIVPAGTWYCAGP
                     IVLLSNVNFHLSANCTIYFSPNPADYAKDGPVNCGANGNLFYSRWQANDCLNYGSPVY
                     ARNQTNIALTGEDATSVLNGQAMTPFAGTGNTSTCWWTYKGSNGAYGCVNSSTPSQAY
                     INPKNVDLRSAAPGISDALYALLTSPATPWQEDQNYLPALSEAGVPIAQRIFGIGHYL
                     RPCMVEFIGCTNVLMENYHTNNTPFWQHHPTDCTNVVIRGVMADSIGPNNDGFDPDAC
                     NNVLCDSVTFNTGDDCIAIKSGKDLDTEYGPAQNHVIQNCTMNSGHGGITLGSEMGGG
                     VQNIYARNLQMLNQNWATNPLNIAIRIKTNMNRGGFVKNFYVDTVTLPNGVNLKGGGY
                     GSAFLSGSPINGTVPVGVVTATAGNPSASQGGLITFDCDYQPANDANRTRPAVIQNVN
                     ISNVQASNVTLNGVTGSCFQAIVAQGPVAFDYNGPAPTPTIPPISGVTISNCNLGTAV
                     SAGPASATTPGPIYAYNVNGITLKNVIIGSTTYNTTVVDQR"
     gene            complement(10168..11460)
                     /locus_tag="VHV99_04570"
     CDS             complement(10168..11460)
                     /locus_tag="VHV99_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3634750.1"
                     /translation="MKIKGIRWWMVSLVAAGIIINYLARNTLSVAAPTLMKELDITTV
                     QYSHVVVAWQLCYAFMQPVAGYVLDTVGTKIGFAAFALAWSLACAAAAWSTGWRSLAF
                     FRGVLGMAEAAGIPAGVKATSEWFPAKERSVAIGWFNIGSSIGALVAPPLVVWALLHG
                     RWQWAFLIVGVAGIVWSVLWMLLYKHPRNQKLLGDAERDYILSGQEAKHSEAGVVKRS
                     WAAMLGSRDFWAIGIPRILSEPAWQTFNAWIPLYMMTERHMNLKEIALYAWMPFLAAD
                     IGCVLGGYLSPLFHKYAKVSLFTSRKMVFVVGALCMIGPACVGLVASPYAAVALLCVG
                     GFAHQTLSGALYAITSDMFGKNEVATATGMGGMAGYLGAAAFTALFGVLVTQIGYSPL
                     FVVLAVFDIVAAAVVCLLARSTDKIPEPHWGAASAVAK"
     gene            complement(11491..>11737)
                     /locus_tag="VHV99_04575"
     CDS             complement(11491..>11737)
                     /locus_tag="VHV99_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3634751.1"
                     /translation="RCVEAEDVGFITVWGVSANKRSWWDNSGAERLGYRPTQDAEVYA
                     EEILARPNPLDALGQRYQGGSFVGIDYSRDDIAGRPF"
BASE COUNT         2274 a         3849 c         3513 g         2101 t
ORIGIN      
        1 ctgcactgtg gtcgtaatcg ccagaatgtt gcgcgagccc accgaaccgt cagcgttgcg
       61 gtagccctcg aacgtgaaac cttccagcgg cggcatctcc ggcgccttga tggtcgcgat
      121 cggcagatct tccagcccag gcagactcgg catgcgaatc acgtgctcgt tgatccagct
      181 gcccttcggc aaggccttga gcgcgtaacc gatcaccacg tagtagcgga tgacctcatc
      241 gccttccgcc agatcggcca acgcgacctt gtgcccctgc gggacacgct cgcgcaacgc
      301 gaggccatcg ggaaatacgg cgccctcacc cagaccgccg tcgttgacga cgatcgcgac
      361 attgtcattg gggtgaacgc gaatatagag gggggattgt tccatcacat cgatccttat
      421 ggcggtttca ggcttggtgc gccgaccatt catacgtcat cctacaacat gggaaagcgc
      481 ttagacgttc cgtatttacc ctatggcgtg cattatgccg cgaaaacacc gttgagttag
      541 tcaatttgtt gttgtacgat gacgtataag aaatctccat ttctacaatt tcactctatc
      601 gccgccctgg atactcatca tgacgacacc gcaggaactc aagcagatcg tttccgaagg
      661 ccttctgtcc ttccccgtta ccgacttcga cgaacaaggc gatttccgcg ccgataccta
      721 tgccgagcgc ctcgagtggc ttgcgcctta cggtgcttcg gccctgttcg tcgccggcgg
      781 cacgggcgag ttcttctcgc tcacgcatga cgactactcg aacgtcgtgc gcacggccgc
      841 tgaagtctgc aagggcaagg tgccgattct cgccggcgcc ggcggcccga cccgcgtggc
      901 gatcgcgtac gcaaaggaag ccgaacgtca cggcgcgaac ggcatcctgc tgatgccgca
      961 ctacctgacc gaagcctgcc aggaaggcat cgcggcccat gcggaagaag tgtgcaagtc
     1021 cgtgccgaac atgggcgtga tcatctacaa ccgcgccaat tccaagctta acgcggacat
     1081 gctcgaaggg ctggccgagc gctgcccgaa cctgatcggt ttcaaggacg gcgtgggcga
     1141 gatcgagaac atggtgacga tccgccgccg cctgggcgac cgtttctcgt accttggcgg
     1201 cctgccgacc gctgaagtct atgccgcggc ctacaaggcg ctgggcgtgc cggtgtattc
     1261 gtcggccgtg ttcaacttca ttccgaagac cgcgatggac ttctaccgcg cgattgccgc
     1321 ggacgaccac gccaccgtcg gcaagctgat cgacgaattc ttcctgccgt atctggcgat
     1381 ccgcaaccgt cgcgcgggct acgcggtcag catcgtcaag gccggcgcga agctggtcgg
     1441 ccacagcgcg ggtccggtgc gcgcgccgct gaccgatctg accgaagaag aaatggccag
     1501 actcgacgcg ctcatcaaga cgctcggccc gcagtaaagc agcacggcgc cacgccgcgc
     1561 gaacctggcc gaagctggac gcgcctggct cggcaccgca aagagcgccc gggacggcaa
     1621 cgtcgccggg cgttttttta gggcgcttgc ataaaaattc aggagatgac gatggaaatg
     1681 agccgcactg cgagccccgc ccacgtcgca aagcgcacca aagtccgcta cgcgattctg
     1741 ctgctgatct ttctgatcac gacgttcaat tacgcggacc gcgccacgct ctcgataacc
     1801 ggatccgcga tgcgcgccga attcggcctc gacgcgatcc gcatgggcta catcttctcg
     1861 gcgttcagct gggcctatgt tctgtcgcaa ctgccggcgg gctggctact cgaccgtttc
     1921 ggcgcgcggc gcgtgtacgc ggcgagcatt tccctgtggt cgctgtttac gctgctgcaa
     1981 agctcgatcg gtctgctcgg cagcgcaggc gccgccgtcg ccgcgctgtt cgtgctgcgc
     2041 ttcgcgatgg gcgcggccga atcgccggcg tttccggcca atgccaaggt cgtcgccagt
     2101 tggtttccca ccaacgaacg cggcaccgcg tccgctattt tcaatgccgc tcaatacttc
     2161 gcggcggtca ttttcacgcc actgatggca tggctcaccc atgcgttcgg ctggcacatg
     2221 gtttacctcg tgatgggcgt ggccggactg ctgctcgccc tcacctggct gaaggtgatg
     2281 aagaaccccg ccgatcatcc gcgggtctcg cgcgccgaac tcgactacat cgaacagggc
     2341 ggcggcgtgg tgagcgggca caggcaagcc gcacgggctg aagacattgc cagcgcccgc
     2401 ggctggtcgc tggtgcgtca actgctgggc aaccgcatgc tgctcggcgt ctatctcgcg
     2461 cagtactgca tcaacgtgct gacctacttc ttcctgacct ggtttccgat ctacctcgtg
     2521 caggcgcgtg gcatgacgat tctgcaagcg ggcctcgtgg catcgctgcc ggcgatttgc
     2581 ggattctcag gcggcgtgct cggcggcatt ttgtccgatg ggatgatccg gcgcggccac
     2641 tcgctcacta ttgcgcgcaa ggtgccgatt gtcggcggca tgctgctgtc ggtgtgcatc
     2701 atcggctgca attacgtgtc gaccgactgg atcgtcgtcg cgctgatgtc gctcgcgttc
     2761 ttcggcaaag gcatcggggc gctcggctgg gccgtcgtgg cggatacgtc gccgaaggag
     2821 gcgttaggtt tgtcgggcgc catcttcaac atgttcggca atgtcgccgg catcgtgacg
     2881 cctatcgtga tcggctacct cgtcgcgaag tccgggtcgt tcaacggcgc gctggttttc
     2941 gtcggcctga atgccctgct gacggtgttc agctacctcg tgatcgtgaa ggacatcaaa
     3001 cgggtcgaac tgcgccggag ctgactaatg ggccaactaa cgggcgagtt aaaggggcga
     3061 gttaaagggg cgagttaaag gggcagttat cgtacttccc cgggggctcc ggcgaaacaa
     3121 cgctgcttgt ccgccaggcg cctgaaaccg ggcctcgaag ccagggctct tgccgatgcc
     3181 caaaatgatt tggcgtttat ataatcagtc gtcagacgac ggtataatga atcgcaggga
     3241 agtacctgca actatctgat ttaaggggcg ttttttcgat ggctacacca ctggcatccg
     3301 cggtaccgcc acgacgcgcg cgcaatcttg ccgagtttgt cgtcagctac gtcagcgagc
     3361 aaattgcgtc caacgccttg aagccaggcg acaaactgcc gaccgaatcg cagctgatgg
     3421 tcacgctgag cgtgagccgc accgtcatcc gcgaggcgat ttcgcgcctg caggcgggca
     3481 aaatcatcga gacgcgccac ggcatcggca gcttcgtgct ggaaccgccg cgcgaaaagc
     3541 tgggtatcga catggtgccc gccaccacct tgcgcgacgt gctgtcgatc ctcgagttac
     3601 gcatcagcct cgaaacagaa tgcgcgggtc tcgcggcgca acgggccaaa gcggacgacc
     3661 tcgcgcgcat gcgcaccgcg ctcgacgcga tcgaagccac ccgccacaac ggcctggata
     3721 gcgtcgatgc cgatctgcag ttccatatct ccgtcgcgcg cgcaacgggc aaccgctatt
     3781 tcgtcgacat cctcacgcaa atgggcagcg cactgattcc gcgcaaccgg ctcgattcgg
     3841 cggggatcgc gcatgcggag ccggacgcgt atctgtcgct cgtcaatctg gagcacgaaa
     3901 gcattctcga ggcgatcacg cgtcacgacg cggaaggcgc gcgcgcggcg atgcgcatgc
     3961 acctgtccaa tagccgcgag cggctgcggc gcgcgaacga agccgttgaa gcgaacagct
     4021 agcggcgaat agctggcgcc tgaagtgaat cgctgatttt ttgcgctgag caactgctgg
     4081 tttattccac cgaacaaccg ctgattcatc acgctcagca gccctgattc atcacgctga
     4141 gcaacgactg attgattacg ctggacgcaa aaatggccgg gatatcccgg ccatttttta
     4201 tttcacgcgg cgcccgcttg cacgccgctc acgcaccgct tagctgcgtg tgatctcgcc
     4261 atcacgacga cgccacaggt ccagcggatt gctgtcggtc agcgcctcgg gcagcagatc
     4321 agccgggaaa tcctgatagc acaccgggcg caggaaacgc tcgatcgagg tcgtgcccac
     4381 cgacgtcgcc cggctatccg acgtggccgg gaacgggccg ccgtgcacca tcgcgtgcga
     4441 aacctcgacg ccggtcggga aaccattgac cagaatccgc ccggccttgc gctcgaggat
     4501 cggcaccagc ttcttcgccg ccgccagatc cgcgctgtcc atctggaccg tcgcggtcag
     4561 ctgaccgtca aagtgctcgg cgaccgcgag catttcctgc tcgtccttgc aacgcacgat
     4621 ggtcgatgca ggcccgaaca cctcgtcctc cagcgccggc gtagccagga aggtttgcgc
     4681 atcggtcacg aacaacgccg cgcgcgcttg cgtggggccc gttgcgccaa tgccttgcgc
     4741 aaccgtttcg acgcccttgg tcgccgccag tttgccctcg ccgtcttcat aggcggcgtg
     4801 gatgccggcc gtcagcatgg tctgggccgg cttgccgctc agcgcctcgg aggccgttgc
     4861 aacgaagcct ttgagcgcgt cgctgtcgat cgcgatcgcg agacccgggt ttgtgcagaa
     4921 ctgccccgcg ccgagcacca gcgaatcgac gaaaccgcgc gcgatgctct cgccgcgttg
     4981 cgacagcgca ttcgacagga ggaataccgg gttgatgctg ctcatttccg cgtagactgg
     5041 aatcggctcg gcgcgggcgg ccgccacgcg catcagcgac gtgccgcctg cgcgcgaacc
     5101 cgtgaacccg accgccttga tcgccggatg cgcgaccagt gcttcaccga ccgaattgcc
     5161 ggcgccgacg atcatcgaga acacgccttc aggcaggtcc atgtcgcgcg cgacctgctg
     5221 aatcgcacgg ccgaccatct ccgaggtgcc cagatgcgcg cgatgcgcct tcaccaccac
     5281 cgggcaaccc gccgccagcg ccgcagcggt gtcgccgcct gccaccgaaa aggcgagcgg
     5341 gaagttgctg gcgccgaaca cggcgaccgg ccccagcgga atcttttgca ttctcaggtc
     5401 ggaacgcggc agcggcttgc gctcaggcaa cggcgaatcg agcgttgccg cgagccattg
     5461 gccggcgcgt acgacttgcg cgaacagttt gagctgccct gtcgtgcggc cgcgctcgcc
     5521 ttcgaggcgc gccttcggca ggccggattc ttcgtgcgcg cgttggatca gcgcgtcgcc
     5581 gagcgccgtg atgccgtccg cgatacgctc gagaaactcg gcgcgcaccg tgaggggcag
     5641 cgcgcgatac gggtcgaacg ctttctgtgc gagctcgcac gcgagctcga cgttcgccac
     5701 cgagccactg ccgaacaccg gctcggcgat gtcggatccc gtggccggat tgaacgcacg
     5761 cagtggtttt tcctcgccgc gcaccgcttt gcgaccaatc agcatttcgc cggtaataga
     5821 catttcgttt ccttgaatgg acgtctcaga tataggtcat cctacaacat tgcaaggccc
     5881 tcgcgcaacc tgaaacccct gccccaagag gaattcgggt ttacgtggcc gttacaaccc
     5941 acagagcgaa tggtaatctg ccgtaagatg tacgacgacc tataacaaat aataacaaac
     6001 agtttggcag ccgccagttt tccagataac gcagctcgag gagacatccc catgacagcc
     6061 gtcgcgcctt atcagccttt acccaacagc tttcgccggg ccgtgtgcgg cggcgaaacc
     6121 ctgatcggct gctgggtctc gctcgccagt ccgattgcca cagaactgct cggcgtcgtc
     6181 ggcttcgact ggatgctgct ggatgccgaa cacgcaccga acgacgtcct gacgctgatc
     6241 ccccaattga tggcgctcaa ggacagcccg agcgcgcctg tggtgcggcc gcccgccaac
     6301 gacagcgtat tcatcaagcg gctcctcgat agtggcttct cgaattttct ggtgccgttc
     6361 gtcgagagcg ccgaagacgc cgcccgcgcc gtcgccgcga cgcgctaccc gccgcagggc
     6421 atccgcggcg tgtcggtgag ccaccggggc aatcgctatg gcacggtctc cgactacttc
     6481 gacatcgcta atgacaacgt ctgcgtcatc gttcagatcg aaagccgcaa ggccgtcgac
     6541 aatatcgacg agattcttgc cgtggatggc atcgatggcg tgttcgtcgg gccgtccgat
     6601 ctggcggctt cctacggcca ccttggcaac ctgaatcatc ccgaggtaca gcaggcgatt
     6661 gcgcgcatct tcgagcgcgc ccaggccgcc ggcaagccga gcggtattct cgcgcccgtg
     6721 caggccgacg cggagcgcta tctctccatg ggctgccgcg tcattgcggt ctgtgcggac
     6781 atggggctgc tgaaagccgg cgcgcaggcg gttcaaaaac acttcatgca gaaacaggcg
     6841 gccccgaagt aaacacggtc gccatgacat ctccggagga ttccatgcaa aaagcaggct
     6901 ttatcggact cggcatcatg ggtaagccca tggccgccaa ccttctcaag aacggcgtcg
     6961 cgctcgcggc gtttacgcgc agcggcgtgc ccgacgatct cacccaggcc ggcgcgctcg
     7021 cgtgcgacag ccccgccgct gtggccgcgc aggccgacgt gatcttcatc atggtgccgg
     7081 atacgccgga cgtcgaacgc gtgctgtttg gcgaacaagg cctcgccggc gccttgcgcc
     7141 ccggacaaac cgtggtcgac atgagctcga tttcgccgat ggccacgcgc gagttcgcgg
     7201 cccgcgtgcg tgagcgcggc gcggactata tggatgcacc ggtttcaggt ggcgaggtcg
     7261 gcgccaaggc cggttcgctg accatcatgg tgggcggcga aaccgcgacc ttcgagcgcg
     7321 tcaagccgct cttcgacatg atgggcaaga acgtcacctt gatcggtgat gtcggcgccg
     7381 gccaggtgtg caaggtcgcc aatcaggtga tcgtcgcggc gacgatcgag gcggtcggtg
     7441 aagcgttgct gctcgcttcc aaggccggcg tcgatccggc gcgcgtgcgc gaagcgctaa
     7501 tgggcggttt tgcgtcctcg cgcattctcg aagtccacgg tgaacggatg acgaaacgca
     7561 ctttcgatcc aggcttccgg atcgagcttc accagaagga tctgaatctc gcgctttcga
     7621 cggctcaggc actcggtgtt tccttgccga atacggccac ctgccaggcg ctcttcaacg
     7681 cctgcgtggc acatggcggc aaggcatggg atcactccgc catggtgcgc gcgctcgaaa
     7741 tactcgccaa tcacgaaatc ggccagcagg ccgactaaac ccgcaggccc gacaccagcc
     7801 gccgctccac caggcttatg caaataggga agatggagcg gcgcatccgc gcgttcacga
     7861 cctgagcgag cgggtctcaa cggcaacagc ttcacgctct gccatagcac tacatccaac
     7921 atattacgga caacttacga ccagggaggc tgtttcgata tgcaccgctt attcaggcat
     7981 cggattgacg aaccgcgtcc catcgagttc tgacgatttc ctcacaacgc ccgcactggg
     8041 caaggagaca tccgtggcaa ccaaagataa aaatcgcggt acgctggcaa catcgagaat
     8101 ccccggtcag ggcgcaccga attcacctaa acgcagagcc tttatcgtgc tcgcaggaac
     8161 ctccacgggc gcaaccctgc tgggtggcct gtccgcctgc ggcgggtccg tcaatcctcc
     8221 cgccacaacg ccaccggcca caccgccggt ggtgcaagac ccgatctggg gcacctcggg
     8281 cgccgcaacg cagatcatcg cgtcgctgca aaacatcacg caggcaatgt tcccggcacg
     8341 cgactatgtg gtgacccagt acggcgcagc gccttgcggg gtcatcgccg cggccaatcc
     8401 ctacaccggg tcgtcatcgc ccacgagccc cggctcggcg caaaccaatg cggcgaacgc
     8461 gcccgacgca aggcctgcgt ttctcgccgc gatccaggca tgcaatgcgg ccggcggcgg
     8521 ccgcgtgatc gtgccggccg gtacctggta ttgcgcgggg ccgatcgtcc tgctcagcaa
     8581 tgttaacttc catctgagcg cgaactgcac gatctatttc agcccgaacc cggcagacta
     8641 tgcgaaggac ggcccggtca actgcggcgc gaacggcaac ctcttctaca gccgctggca
     8701 ggcgaacgac tgcctcaact acggctcgcc ggtctacgcg cgcaatcaga ccaacatcgc
     8761 gctgaccggc gaagacgcca cgtcggttct caacggccag gcgatgacgc cgttcgccgg
     8821 aaccggcaac accagcacct gctggtggac ctacaaggga tcgaacgggg cgtacggttg
     8881 cgtgaattcg tcgacacctt cgcaggccta catcaatccg aagaacgtcg acctcagaag
     8941 tgccgcgccc ggtatttccg acgcgctcta tgcgctgctg accagccccg caacgccttg
     9001 gcaagaggac cagaactacc tgcccgcgct gtccgaagca ggcgtgccga tcgcgcaacg
     9061 gattttcggc atcggacatt atctgcgtcc gtgcatggtc gagtttattg gctgtaccaa
     9121 cgtgctgatg gagaactatc acaccaataa cacgccgttc tggcagcacc atccgaccga
     9181 ttgcacgaac gtcgtgattc gcggcgtgat ggccgacagc atcggtccga ataacgatgg
     9241 attcgatccg gacgcgtgca acaacgtgct ctgcgacagc gtcacgttca ataccggcga
     9301 cgattgcatc gcgatcaagt ccggcaagga cctcgatacg gagtatggtc ccgcgcagaa
     9361 tcacgtgatc cagaactgca cgatgaacag cggccacggc ggcatcacgc tcggcagcga
     9421 aatgggcggc ggcgtgcaga acatctatgc gcgcaacctg cagatgttga atcagaattg
     9481 ggccaccaat ccgctgaaca tcgcgatccg tatcaagacg aatatgaatc gcggcggctt
     9541 cgtgaagaac ttctatgtcg atacggtcac gctgcccaac ggtgtcaatc tgaaaggcgg
     9601 cggctacggc agcgcgtttc tgagcggcag tccgatcaat ggcacggtgc cggtgggcgt
     9661 cgtcacggcg acggcgggca atccctccgc gtcgcagggc ggcctgatca cgttcgactg
     9721 cgactatcag ccggctaacg atgcgaatcg cactcgcccc gcggtcatcc agaacgtgaa
     9781 catttccaat gtgcaggcaa gcaacgtcac gctcaacggg gtgacgggtt catgtttcca
     9841 ggcgatcgtg gctcaggggc ccgtcgcttt cgactacaac ggtcccgccc cgaccccgac
     9901 cattccgccg atcagtggcg tgacgatctc gaactgcaac ctgggaacag cggtcagcgc
     9961 cggacccgca agcgcgacga cgccagggcc gatctatgcc tacaacgtca atggaatcac
    10021 gttgaaaaac gtaatcatcg gttcgacgac ttacaacacg acggtcgtcg atcagcgtta
    10081 agccagaggt aaaaaaatgg cggtgtccaa gttggacacc gccattttca ctacccggcg
    10141 cgcttcactg ccggatcaga gcacagctta ctttgcgaca gcactcgctg ccccccaatg
    10201 cggctcggga attttgtcgg tgcttcttgc cagcagacac accaccgcag ccgcgacgat
    10261 atcgaacacc gccagcacca caaacaacgg gctgtagccg atctgcgtga ccagcacgcc
    10321 gaacagcgcc gtgaacgccg ccgcgccgag ataacccgcc atgccgccca tgcccgtggc
    10381 ggtggcgact tcgttcttgc cgaacatgtc ggaggtgatc gcgtacagcg cacccgacaa
    10441 ggtctggtgt gcaaagccgc cgacgcacag cagcgcgacg gccgcgtaag gactcgccac
    10501 caggccgacg caggcgggcc cgatcatgca gagcgcgccg acgacaaaga ccatcttgcg
    10561 cgacgtgaac agcgagacct tcgcgtactt atgaaacagc gggctgaggt agccaccgag
    10621 cacacagccg atatccgccg ccagaaaagg catccacgcg tacagcgcga tttccttcag
    10681 gttcatatga cgctcggtca tcatgtacag cgggatccac gcgttgaagg tctgccaggc
    10741 cggctcggaa agaatccgcg gaatgccgat ggcccagaag tcgcggctgc cgagcatggc
    10801 cgcccaactg cgtttgacca cgccggcttc gctgtgcttc gcctcctgtc cgctgaggat
    10861 gtagtcgcgc tcagcgtcgc ccagcagttt ctgattgcgc gggtgcttgt aaagcagcat
    10921 ccacagcacg ctccagacaa tccccgccac cccgacgatc aggaaagccc attgccagcg
    10981 accgtgcaac aacgcccaaa cgaccagcgg cggcgccacc agcgcgccga tggacgagcc
    11041 gatattgaac cagccgatcg cgacggaacg ctccttggcc gggaaccact cgctcgtcgc
    11101 cttgacgccg gccgggatgc ccgccgcttc ggccataccg agcacgccgc gaaagaacgc
    11161 aaggctgcgc cagcccgtcg accatgccgc cgcggcacag gccagcgacc aggccagcgc
    11221 gaacgcggca aagccgatct tggtgccgac ggtgtcgagc acatagcccg cgaccggctg
    11281 catgaatgca tagcaaagtt gccaggccac cacgacgtgc gaatactgca cggtcgtgat
    11341 gtcgagttcc ttcatcagcg tcggcgccgc cactgacaac gtattgcgcg cgaggtaatt
    11401 gatgatgatc ccggccgcga ccaggctgac catccaccag cggatgccct tgattttcat
    11461 gactacgttt cctcgtattt cctgggccga tcagaacggc cggcccgcaa tgtcgtcacg
    11521 cgaataatcg atgccgacga aactgccgcc ctggtagcgc tgacccaagg catcgagcgg
    11581 attcggccgc gccagaattt cctcggcgta gacctcggcg tcctgcgtcg gtcgataacc
    11641 caaacgctcg gcaccgctgt tgtcccacca gctgcgctta ttggccgaca caccccacac
    11701 ggtgatgaag ccgacgtcct ctgcttcgac acagcgg
//
LOCUS       DATASH010000153         3276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1604555,
            whole genome shotgun sequence.
ACCESSION   DATASH010000153 DATASH010000000
VERSION     DATASH010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1604555"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VHV99_04580"
     CDS             <1..289
                     /locus_tag="VHV99_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007733835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX3634752.1"
                     /translation="SALIGRVPLKTVAHMVLSRASLSATQALQAGLISHVVASGELDA
                     EADALTATLLTCSPVTLRAVKQFLHLAPEMPAASASGFAAHLAATALSARF"
     gene            355..1920
                     /locus_tag="VHV99_04585"
     CDS             355..1920
                     /locus_tag="VHV99_04585"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007733837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX3634753.1"
                     /translation="MKPSAEIPHTATGASLPDLERFGLRRFLESLDEDELERHDNPVE
                     LADIAALMEGNGRAVWIRQPSGDSISLAGNVTASRSRLARAFGTTPDKLLDVVRERLR
                     SKGQLVEVSREQAPVQQVVLTGEECDFTKLPVHLQHDLDGAPYVSASIDFVVDPESGW
                     TNVGVRRLMLRGRRTAGIDLVAPSDLRAIAIAHARRGERLPIAFAVGTHPIDHVGATM
                     RIPADELELVAALRGAPMGVVKCVTSDLRVPADAEFILEGYLDERGHSEPEGPYGEFL
                     GYYGGIKTNPVFHLTAITHRRDAIFQTCTIGGRSMARTDTAQLAALRTEVTVWRALET
                     AVREVNAVYAGPATGGMFNVRVAMQQRVPGEARNAIAAVFASLANVKHVFVVDPDIDI
                     FSDEQMDWALATRFQADRDLVLQTGLRAMPLDPSLNGATQTAKAGFDLTLPLLKPGTQ
                     RSLEHRVPTPPAYAGARFETLEAALEDGPKRFVDLMSATGTRDGREIVRWLEDVAVKR
                     PLSRDEEGRYGFA"
     gene            2081..2989
                     /locus_tag="VHV99_04590"
     CDS             2081..2989
                     /locus_tag="VHV99_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3634754.1"
                     /translation="MNLSLKQLKVFLGVANASSFTKTAQSMHLSQAALSAIIRELETQ
                     LQCRLLERTTRTVALTDAGRMFFPTAVKIVETLEKSVIELNELGRQKQASLLLGCTPM
                     IAASLMPPVLARFAQAHPQSQIELVDRPPSELHRMVEEGDLDAAFGIFFSQLSGIDRV
                     PLFPVQLVAVSALDNAKPGHATQCNWRALEGVPLITLPKDNPLQRLIEATLAKEEVTS
                     GKRIVVAHMQTAIAMAHAGLGVAVMPSFCEHICQHYQVRVDQMHPPVEFSFYRITRAG
                     RGELAVLDQFSEMFAQAAQKRPVGLS"
BASE COUNT          630 a         1034 c         1002 g          610 t
ORIGIN      
        1 gtccgcgttg attggccgcg tgccgttgaa aacggtcgcg cacatggtcc ttagccgcgc
       61 ttcgctaagc gcaacgcaag cgctgcaggc ggggttgatc agccacgtgg tggcgtcggg
      121 cgaactggat gccgaagccg acgcgcttac tgcgaccctg ttgacatgca gccccgtgac
      181 gctgcgtgcc gtgaagcagt tcctgcatct ggcgccggaa atgccagccg cgagcgctag
      241 cggttttgca gcccacctgg ccgccacggc tttatcggca cgtttctgaa tgaccggcca
      301 cgtactcgac attgaaccaa cgttgaaccc gagttgaact aaaggaccac gacattgaaa
      361 cccagcgctg agattccaca caccgcaacc ggcgcgagcc tgcccgatct ggaacggttc
      421 ggactacgcc gctttcttga atcgctcgat gaagacgaac tcgagcgtca cgacaacccg
      481 gtcgaactcg ccgacatagc agcgctgatg gagggcaacg ggcgcgcggt gtggatcagg
      541 cagccatcgg gcgacagcat ctcgctcgca ggcaatgtga ctgcgagccg atcgcgcctc
      601 gccagggcat tcggcaccac gcctgacaag ttgctcgacg tcgtgcgcga gcgtttgcgc
      661 tccaagggac aactcgtgga agtgagccgc gagcaagcgc ccgtccaaca agtcgtgctc
      721 actggcgaag aatgtgattt caccaagctg ccggttcacc ttcaacacga tctcgatggt
      781 gcaccctatg tgtcggcatc gatcgatttt gtcgttgatc cggagtccgg atggaccaac
      841 gtcggtgtgc ggcgcctgat gctgcgcggc cgccgcactg cggggatcga cctggtcgcg
      901 ccgtcggacc tgcgcgccat cgccatcgcg cacgctagac gcggcgaacg cttgccgatt
      961 gcattcgccg tcggcacgca tccgatcgat cacgtgggcg ccaccatgcg aattccggcc
     1021 gacgaactgg aactcgttgc cgcgctccgg ggcgcgccaa tgggcgtcgt caaatgcgtg
     1081 accagcgacc tgcgcgtgcc cgccgatgcc gagttcatcc tcgaaggcta tctcgacgaa
     1141 cgcggccatt cagagcccga aggcccatat ggcgaatttc tcggctatta cggcggcatc
     1201 aaaacgaatc cggtgtttca cctcaccgcc attacgcatc gtcgcgacgc gatattccag
     1261 acctgcacca tcggtggccg cagcatggca cgcacggaca ccgcccagct tgcggcgttg
     1321 cgcaccgaag tcaccgtctg gcgcgcgctc gaaaccgccg tgcgagaagt caacgcggtc
     1381 tatgccggac ccgcaaccgg cggcatgttc aacgtgcgcg tcgccatgca gcaacgtgtg
     1441 ccgggcgagg cacgaaacgc gatcgccgcg gtgttcgcgt cgctcgcaaa cgtgaagcat
     1501 gtgttcgtcg tggatccgga tatcgatatt ttctccgacg aacaaatgga ctgggcgctc
     1561 gctacccgtt tccaggcgga ccgtgatctg gtgttgcaaa ccggcctgcg ggccatgccg
     1621 ctcgatcctt cgctcaacgg cgccacgcag accgcgaaag ccggcttcga tctgaccttg
     1681 ccgttgctca agcccggcac gcaacgttcg ctcgaacacc gcgtgccgac gccaccggcc
     1741 tatgccggcg cgcggttcga aaccctggag gccgcgctcg aagacggacc gaagcgcttc
     1801 gtcgatttaa tgtccgcaac cggcacgcgg gatggccgcg aaatcgtccg ctggctagag
     1861 gatgtggcag taaagcgccc gctcagccgc gatgaagaag ggcgttacgg gttcgcgtga
     1921 agttagcgtg aagttcccgt gaaggtagtg ctcctgtagg gaagtgacac gcaaggccgg
     1981 ggcgcattgg ttgcgcctcg cctgagcggt tcgtgtgcgg gcgggacgag tagaattcac
     2041 ctacgctcgg attgtccgat atacgaagga aaagtcgcag atgaatttgt ccctcaagca
     2101 gctcaaggtt tttctcggcg ttgcgaacgc atccagcttc accaagaccg cgcagagcat
     2161 gcacctgagt caggccgcgc tcagtgccat catccgcgag cttgaaacgc aattgcagtg
     2221 ccgcttgctc gagcggacca cgcgcacggt ggcgctgacc gatgcgggcc ggatgttttt
     2281 tccgaccgcg gtcaagatcg tggagaccct ggaaaagtcg gtgatcgagc tgaacgagct
     2341 tggccggcag aagcaggctt ccctgctgct cggatgcacc ccgatgatcg ccgcgagcct
     2401 gatgccaccc gttctggccc gcttcgcgca agcgcatccg caatcgcaga tcgaactcgt
     2461 cgaccggcca ccgtcagagt tgcaccggat ggtggaggaa ggcgacctgg atgcggcgtt
     2521 tggcatcttc ttctcccagc tctcgggaat cgaccgtgtt ccgctgtttc ccgtgcagct
     2581 ggtggcggtt tcggcgctgg acaacgccaa gccgggccat gccactcagt gcaactggcg
     2641 tgcgctggag ggtgtgcccc tgattacgtt accgaaggac aaccctttgc aaagactgat
     2701 cgaggcgacg cttgccaagg aagaggtgac ctctggaaag cgcatcgtgg tcgctcatat
     2761 gcagacggcg atcgccatgg cgcacgcggg tctcggcgtc gccgtcatgc cctcgttctg
     2821 cgaacacatc tgtcagcatt atcaagtgcg cgtcgaccag atgcatcccc cagtcgagtt
     2881 ctcgttctat cgcatcacgc gcgccggccg cggtgagctc gccgtactcg atcaattcag
     2941 cgaaatgttc gctcaagccg cgcaaaagcg ccccgtcgga ctttcttagg ggctttgtca
     3001 acgtcccggc agcagacgcc cgacggccgg tgtgacgacc cgtcagaaaa cggtcgacgg
     3061 aatctggggg atttcaacaa gttcgcgcca agcgacgcga gccgttcgaa cgcagggaaa
     3121 tcgatcttct catcaggggt cattggcgtg agcaacccga cgattgatcc ctgcagcaaa
     3181 ggcgaatctg tcatggcaac tgctcctgat ctgatgcgtc gagcatcatg ccttttgtct
     3241 tcgcacgctg ggcaatcaac tccctgagcg gctcgt
//
LOCUS       DATASH010000154         2411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6153179,
            whole genome shotgun sequence.
ACCESSION   DATASH010000154 DATASH010000000
VERSION     DATASH010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6153179"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="VHV99_04595"
     CDS             complement(<1..352)
                     /locus_tag="VHV99_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX3634755.1"
                     /translation="MKACNAVKLLVVVGCVTLTSNVYAQASDAVSVTSSPAAESGKAT
                     PADKKLARDVRKALSKAPNFNVSNVFVKARGGLVTLSGSVSDGPQLQQAEEVTKGVPG
                     VTSVSNHLTLYTKGY"
     gene            complement(441..1433)
                     /locus_tag="VHV99_04600"
     CDS             complement(441..1433)
                     /locus_tag="VHV99_04600"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEX3634756.1"
                     /translation="MKVAIFSSTRYERRYLDEANRSGEHTLHYFDVLLDMDTVGLAAG
                     YGAVCIFVNDKADAQVLSALQKGGTRLLALRCTGFNNVDLEAAARLGMKVVRVVTYSP
                     NSVAEHAVALLLAINRKVHRAYNRTRDSNFSLDGLTGFDLCGKTVAVVGTGKIGRVFA
                     KIMLGFGCRVIGFDPQPSAEFEALGLSYAKPGEIGEKADIISLHCPLTPTTHHVINSE
                     TLKRAKQGAILINTSRGGLIDTEAVIEALKTGKLGGLGIDVYEQEGNLFFRDLSSEII
                     TDDVFQRLVSFPNVIVTGHQAYLTHEALTTICETTLESVSAFEKNLPLQNEVKL"
     gene            complement(1554..>2411)
                     /locus_tag="VHV99_04605"
     CDS             complement(1554..>2411)
                     /locus_tag="VHV99_04605"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit EIIB"
                     /protein_id="HEX3634757.1"
                     /translation="TLGMRMGFTFSQGGIDFLMFNLIGGKATHAWYVLILGPIYAAIY
                     YCVFRFVITRFNLKTPGREDDTAETIAISTAGTGGRSRELVLAFGGRSNITSLDACIT
                     RLRISVKDPALVDEAKLKALGAAGVVRVGNGVQAIFGPLSENMKTDMQEYLKTAGSDA
                     DLAPAGTPVAEGAQDAGVASPAAAAASVMATAAAQSSAQQIARAEKIRAALGGAANII
                     KLDALAATRLRVELSDASRLDAAALKAAGVPATQALKNGAVDLLVGLGAENLAGAIQW
                     ELATHPAPA"
BASE COUNT          432 a          774 c          702 g          503 t
ORIGIN      
        1 aatacccttt cgtatagagc gtcagatggt tgctcaccga cgttacaccg ggcactccct
       61 tcgtcacttc ctcagcctgc tggagctgag gaccgtccga cacgctgccg gacaaagtca
      121 cgagaccgcc gcgcgccttg acgaatacgt tcgacacgtt gaagttgggc gctttggata
      181 acgccttgcg tacatcccgc gccagcttct tgtccgctgg cgtagccttg cctgactctg
      241 cagccggact cgacgtcacc gacacggcgt cgctggcttg cgcgtagacg ttcgacgtca
      301 gcgtcacgca tccgacaaca acgagcagct tcacggcgtt gcatgccttc atgatggcct
      361 cgcatgttcg ccctggcaga tatcgaatgc gccgtgcgcc ttcgccagcg agttcacagc
      421 gtgcgttgca cgcgccttta tcagagcttc acctcgttct gcaaaggcag attcttctcg
      481 aacgccgaga cgctttccag cgtcgtttcg cagatcgtcg tcaatgcctc atgtgtcagg
      541 tacgcctgat gtccggtgac gatgacattc ggaaacgaga cgaggcgttg aaagacgtca
      601 tcggtgatga tctcgctgga gagatcgcga aaaaaaagat tcccttcctg ttcgtacaca
      661 tcgatcccca gtccgccgag cttcccggtc ttcaacgctt cgatgacagc ctcagtgtcg
      721 atcagcccgc cgcggctggt attgatcagt atcgcgccct gcttcgcgcg tttgagcgtc
      781 tccgaattga tgacgtggtg tgtcgtgggt gtgagcggac aatgcagcga tatgatgtcg
      841 gctttctccc ctatctcgcc cggcttcgca tagctcagac cgagcgcctc gaattccgcc
      901 gacggttgcg gatcgaaacc gatcaccctg cagccgaagc cgagcatgat cttcgcgaac
      961 acgcggccga tcttgcccgt tccgaccacc gccaccgtct tgccgcaaag gtcgaagccg
     1021 gtcaggccgt cgagcgagaa attcgagtcg cgggtgcgat tgtaggcacg gtgcactttg
     1081 cggttgatcg cgagcagtag cgctaccgcg tgttcggcca cggagttcgg cgaatacgtc
     1141 acgacgcgca cgaccttcat gccgagcctt gctgcagctt cgagatcgac gttgttgaag
     1201 cccgtgcaac gcaaagcgag tagacgcgtc cccccctttt gcagcgcgct cagtacttgc
     1261 gcgtcggcct tatcgttgac gaagatgcag acggcgccat acccagcggc aagaccgacg
     1321 gtgtccatat ccagcaaaac gtcgaagtaa tgcagcgtgt gctcgccgct cctgttggcc
     1381 tcgtcgagat accggcgttc atagcgcgtc gagctgaata tcgccacttt catgatggac
     1441 tccttctgta tccgggttgg gctttcgtcg cctgcggcgc aggtgtgtct agcttgcgca
     1501 ttggaagccg cctctgctat ggaaacatcg ttcatggtct ctgtactcgc atttcacgcc
     1561 ggggctggat gggtcgccag ctcccattgg atcgcgccag ccaggttctc cgcgccgagt
     1621 ccgaccagca gatccaccgc gccgttcttc aatgcttgcg tcgcgggcac gcccgccgct
     1681 ttcagcgcgg ccgcatccag ccgggaggcg tcggatagtt cgacgcgcaa gcgcgtggcc
     1741 gcaagcgcat cgagcttgat aatgttcgcc gcgccaccga gcgccgcacg aatcttctcc
     1801 gcacgcgcga tctgctgcgc cgagctttgc gctgccgctg tcgccatcac tgatgccgcc
     1861 gcagccgctg gactggccac tcccgcgtct tgcgcacctt ccgcgacagg cgtgccggca
     1921 ggcgcgaggt cggcatcgct gccggccgtc ttcagatact cctgcatgtc ggttttcatg
     1981 ttctccgaca gcggaccgaa gatcgcctgc acgccgttgc cgacgcgcac cacgcctgcc
     2041 gcgccgagtg ccttcagctt tgcttcgtcg acgagcgcgg ggtctttcac ggatatgcgc
     2101 agacgcgtga tgcacgcatc gagactcgtg atattcgaac ggccgccgaa agcgagcacg
     2161 agttcgcgcg agcgtccgcc ggtgccggcc gtgctgattg cgatggtttc agccgtatcg
     2221 tcctcgcgac cgggcgtctt cagattgaac cgggtgatca cgaagcggaa aacgcagtag
     2281 tagatcgccg cgtaaatcgg accgagaatg agcacgtacc acgcatgtgt ggccttgccg
     2341 ccgatcagat tgaacatcag gaagtcgatc ccgccttgcg agaacgtgaa gcccatgcgc
     2401 atgcccagcg t
//
LOCUS       DATASH010000155         1835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3187585,
            whole genome shotgun sequence.
ACCESSION   DATASH010000155 DATASH010000000
VERSION     DATASH010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3187585"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            233..715
                     /locus_tag="VHV99_04610"
     CDS             233..715
                     /locus_tag="VHV99_04610"
                     /inference="COORDINATES: protein motif:HMM:NF016310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X"
                     /protein_id="HEX3634758.1"
                     /translation="MSAGLALRPSIFRESPSCIEGIGTAPTSDQLKHFGAAMASFGSI
                     AMFHLVGITPQASRLQDVGGDKLPVLHTVGRDDVDALTKSYAVDKNVDVVVFSAPQLS
                     LYELRFNPVIKIFYDRLVIACKSKKVAFVACIAQASKTNSAADAVAGATVLLAAHRRA
                     "
     gene            877..>1835
                     /locus_tag="VHV99_04615"
     CDS             877..>1835
                     /locus_tag="VHV99_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013690645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3634759.1"
                     /translation="MIHDIPDHSVTSTRVRNSPRFLLFLICLFASAGQLAIDIYVPAL
                     PAMARFFATSPHAIQSSVSGYMAAYAVGQLVFGPIADAYGRKRVLAFGLVIFTIGCLL
                     SLAASNLETFLLARCLQGFGIATTNLLAKAIITDSFSGQALLHAFTYMAIAWGLAPIV
                     APVIGAHLQTWFGWQACLVFLLIYSVVMWGLLWRYRETLPRPVCLEPRTLMTNAGKVL
                     SSPVFQSCFLAQGLCYSILLVFNIVGPFMVQNTLHKPPTYFGYLALGIGMMYFLGGLS
                     NRVHSRRLPTAEQRLYIGAWLMAAAAIAMLLLALTVGLRVWTLA"
BASE COUNT          302 a          564 c          562 g          407 t
ORIGIN      
        1 ccgcgcgatt acgcgggcga gtgaactggg cggcataaag cccaatacct tcgattcata
       61 cgatctcgtt ttcgtttgcc attcgaagta gggcggttcc ggaaaggcct tcgacgcctg
      121 gaacgagaca aggatcgtcg ctcgcctgcg tggctggggc agcaggtcgg cgacgccgca
      181 cctggcgcgg ccgctgagcg gtgcggcgga ccgagacagc cctatgtgtc tcgtgtctgc
      241 cggcctggct ctgaggccat caatattcag agagtcgcct tcatgcatcg agggaatcgg
      301 caccgcgccg acgtcggacc aattgaagca cttcggtgcc gcgatggcga gctttggctc
      361 gattgcgatg tttcatctgg tcggcatcac gccgcaagca tcgcggcttc aggacgtcgg
      421 tggcgacaag cttccggtgc tgcatacggt gggccgggat gacgtcgacg cgctgacgaa
      481 aagctatgcc gtggacaaaa acgttgacgt tgtcgtgttc tccgcaccgc agctgagctt
      541 gtacgaatta cgcttcaatc ctgtcatcaa gatcttctac gaccgtctcg tcattgcctg
      601 taaatcaaag aaggtcgcct tcgtcgcatg catcgcgcaa gcatcgaaga caaactcggc
      661 ggcggatgcg gttgcgggcg caacggtctt gctggcggcg catcgtcggg cctgacgccg
      721 tgtgcacgtc gactgtcaat ttgcgcgcgt gcgcgggaaa tcgcgtcaac ccgtgagatg
      781 acaggcacaa tacgcggcga tgacgccgca catgcgttcc gttcgacatt cggaccaact
      841 gacgcggcgt cgccggaatc cctttttgag acagtgatga tccacgatat ccccgaccac
      901 tccgtcactt ccacccgcgt acggaactcg ccgcgcttcc tgctgttcct gatctgcctg
      961 ttcgcttccg ccgggcagct cgcgatcgac atctacgtgc ccgcgctacc cgcaatggcg
     1021 cgtttcttcg cgacttcgcc tcacgcaatc cagtcgagcg tttccggcta catggcggcc
     1081 tacgcagtcg gccagctcgt tttcgggccc atagccgacg cgtacgggcg caagcgcgtg
     1141 ttggctttcg gcctcgtaat ctttacgatc ggctgcctgc tgtcgctcgc cgcgtcgaat
     1201 ctggagacgt tcctccttgc ccgctgcctg cagggcttcg gcatcgccac caccaacctg
     1261 ctcgcgaagg cgatcatcac tgattcattc tccgggcagg cgctgctgca tgcgttcacc
     1321 tacatggcaa ttgcgtgggg gcttgcgccg atcgttgcgc cggtgattgg tgctcacctg
     1381 cagacgtggt ttggctggca ggcctgcctc gtctttctgc tcatctattc ggttgtgatg
     1441 tgggggctgc tgtggcgcta tcgtgaaacg ttgccgcgcc cggtgtgcct ggagccgcgc
     1501 acgctgatga caaacgcggg aaaggtgctc tcgagcccgg tattccagag ttgcttcctc
     1561 gcccagggtc tgtgctacag cattttgctc gtgttcaaca tcgtcggacc gttcatggtg
     1621 cagaacacgt tgcacaagcc accgacttat ttcggctacc tggcgctcgg catcgggatg
     1681 atgtatttcc tgggcggcct gtcgaaccgc gtgcacagcc ggcgtctgcc gaccgcggaa
     1741 cagcggctgt acatcggtgc gtggttgatg gcggctgcgg cgattgcaat gctattgctt
     1801 gcgttgacgg tcggcctgcg cgtatggacg ctcgc
//
LOCUS       DATASH010000156         5027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2593505,
            whole genome shotgun sequence.
ACCESSION   DATASH010000156 DATASH010000000
VERSION     DATASH010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5027
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2593505"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VHV99_04620"
     CDS             complement(<1..641)
                     /locus_tag="VHV99_04620"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEX3634760.1"
                     /translation="MSEVKSLTPALAAKVERLDALLDSIAARHANVKLASSLAAEDML
                     LTHAILSRGVKIGIFSLNTGRLHAETLGMLDRVKERYDYNIEQFHPQAAAVDEYVASH
                     GLNAFYESVDLRKRCCEIRKVEPLNRALSDVSAWVTGQRREQSVTRAELHAEEHDAAR
                     NIAKFNPLTDWTEAEVWDYLKAFDVPVNPLHARGYPSIGCEPCTRAVRPGEDSR"
     gene            complement(638..1177)
                     /locus_tag="VHV99_04625"
     CDS             complement(638..1177)
                     /locus_tag="VHV99_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007737195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HEX3634761.1"
                     /translation="MASIIKNRAIVNDDWTVVRAAEDGTLPAVNELPAGKVLVPLALW
                     QASRDALIASRSAAEIGVWLAPESEPADIVADFDTIALIGVDFPVFRDGRGYSIGRLL
                     RERYGYKGELRAIGDVLRDQLTFMFRCGFDAYALRSDKDFDDALKAFDEFSLNYQGAV
                     NSSPLFRRREAGELAKASV"
     gene            complement(1200..2879)
                     /locus_tag="VHV99_04630"
     CDS             complement(1200..2879)
                     /locus_tag="VHV99_04630"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015601281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HEX3634762.1"
                     /translation="MYQYDQYDQTIVDERVEQYADQVRRRLSGELSEDEFRPLRLQNG
                     LYLQRHAYMHRIAIPYGNLRSDQMRVLARIAREHDRGYGHFSTRSNIQYNWIKLEETP
                     EILRKLAAVQMHGIQTSGNCIRNITADQFAGVAPDEVVDPRPWAEILRQWSTFHPEFA
                     WLPRKFKIAVSGSKEDRAAVQIHDMGVYLKKNEQGELVADILAGGGMGRTPIIGAIIR
                     RDLPWQHLLTYCEAVLRVYNRYGRRDNMYKARIKILVKALSPEKFSAQVEEEWQHLKD
                     GPSTLTQAEVDRVSQYFQPPVYEKLADTDTSFEKHLLESRAFARWVERNVRPHKVSGY
                     SSVTLSLKPTTIAPGDATDVQMEAVADWADEYSLGEIRVSHEQNLILANVKKRDLFAL
                     WEKAKAQGFATPNIGLLTDIIACPGGDFCSLANAKSIPIALAIQERFNDLDYVYDLGD
                     VSLNISGCINACGHHHVGNIGILGVDKDGSEWYQVTLGGEQGTGATGAHLGRVIGPSF
                     SAEEMPDVMSKVIDTFVENRHEGERFIETYNRIGITPFKERVYASRQPAHA"
     gene            3101..4042
                     /locus_tag="VHV99_04635"
     CDS             3101..4042
                     /locus_tag="VHV99_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HEX3634763.1"
                     /translation="MNLHQFRFVREAVRQNFNLTEAAKALYTSQPGVSKAIIELEDEL
                     GVEIFTRHGKRVRSLTEPGRIILQSVEKILQEVESLKRVGKDYAAQDQGNLVIAATHT
                     QARYSLPAAIAEFKKRFPKVHLSILQGNPTQVAEMVIHDQADLAIATEAIASYKELVS
                     LPCFQWHHVAVMQPDHPLLDRKLLSLDDLTQYPLITYDNAFAGRTKINEAFRLRGLHP
                     DIVLEAIDADVIKTYVELGLGVGIMADIAFNAERDRHLRAMPVGHLFGSNVTRVALKQ
                     GAYLRSYVYTLVELLSPSMNRKLIEQALKGEHETYEL"
     gene            4086..>5027
                     /locus_tag="VHV99_04640"
     CDS             4086..>5027
                     /locus_tag="VHV99_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3634764.1"
                     /translation="MTSYKKKISGLTAAFGFGALLLATAAHADIKVGIDLSSTGPAAV
                     IGITSKNAMLMWPATIAGQKADYIFLDDASDPGVAVRNIRKLINEDHVDVIVGPNITP
                     AAMAALDPVAESQTPMITLIGSASVVEPQEGKKIWAYKMAQTDSAMADVMTRYMSNHN
                     VKTVGFIGFADGYGESWLNEFSKFAALRHIQLVATERYNRTDASVTGQILKLMAAKPD
                     AILIAGAGTPTVLPQRTLIERGFKGPIYQTHGIATPEFIKLGGKDVEGTLFPTQPVVV
                     ARTLPADHPAKKAALAFTDQYEAKYGPGSVTQFAGDAA"
BASE COUNT          991 a         1564 c         1483 g          989 t
ORIGIN      
        1 cggctatctt caccgggacg cacggcgcgc gtacaaggct cgcagccaat gctcggatag
       61 ccgcgcgcat gcagcggatt gaccggcacg tcgaacgctt tgagataatc ccacacttcc
      121 gcttcggtcc agtccgtcaa cggattgaac ttggcgatgt tgcgcgcggc gtcgtgttct
      181 tccgcgtgca gttcggcacg cgtcacggac tgctcgcggc gttggcccgt cacccacgcg
      241 ctgacatccg acagcgcgcg gttcaacggc tctaccttgc ggatttcgca gcagcgcttg
      301 cgcagatcga cgctttcgta aaacgcattc aagccgtgcg aggcgacata ttcatcaacc
      361 gcggcagcct gcggatgaaa ctgctcgata ttgtaatcgt agcgctcctt cacacgatcg
      421 agcatgccga gtgtttcagc atgcagacgg cccgtgttca gcgagaaaat gccgattttc
      481 acgccacgcg acagaattgc gtgcgtgagc agcatatctt ccgcggcgag gctgctggcg
      541 agcttcacgt tcgcgtgacg cgccgcgatc gaatcgagca gagcatcaag gcgctcgacc
      601 ttcgcagcga gtgccggcgt gagcgacttg acttcgctca taccgaggcc tttgccagtt
      661 cgcccgcttc gcggcggcgg aacaacggcg acgagttcac ggcgccctga tagttgaggc
      721 tgaattcgtc gaatgctttc agtgcgtcgt cgaagtcttt gtccgaacgc aatgcataag
      781 cgtcgaagcc gcaacggaac atgaacgtca gttgatcgcg cagcacgtcg ccgatcgcgc
      841 gcagttcgcc cttgtagccg taacgctcgc gcaacaggcg gccgatgcta tagccacggc
      901 cgtcacggaa caccgggaag tccacgccga tcagcgcgat cgtgtcgaag tcggcgacga
      961 tatccgccgg ttcgctttcc ggtgcgagcc acacgccgat ttcagcggcg ctacgcgatg
     1021 cgatcaatgc atcgcgggaa gcctgccaca gcgcgagcgg caccagcacc ttgccggccg
     1081 gcagttcatt gactgcgggc agcgtgccgt cttcagcggc gcgcaccacc gtccagtcat
     1141 cgttgacgat cgcgcgattc ttgatgatag aagccatctg ttcagtatcc ttttcgcggt
     1201 tacgcgtgag ccggttgacg cgaggcgtac acgcgttcct tgaacggggt gatgccgatg
     1261 cggttgtacg tttcgatgaa gcgctcgcct tcgtggcggt tttccacgaa cgtatcgatc
     1321 accttgctca tcacgtccgg catttcttcc gccgagaacg acgggccgat cacgcggccg
     1381 agatgcgcgc cggtggcgcc cgtaccctgc tcgccgccga gcgtcacctg ataccactcc
     1441 gagccgtcct tatcgacgcc cagaatgccg atgttgccga cgtggtggtg accgcaagca
     1501 ttgatacagc ccgagatgtt caacgacacg tcgccgaggt cgtacacata gtcgaggtcg
     1561 ttgaaacgtt cctgaatcgc gagcgcaatc ggaatcgact tcgcattcgc gagcgagcag
     1621 aaatcgccgc ccgggcacgc gatgatgtcg gtcagcaagc cgatgttcgg cgtcgcgaaa
     1681 ccttgcgcct tggccttttc ccacagcgcg aacagatcgc gcttcttcac gtttgcgaga
     1741 atcagattct gttcgtgcga cacgcggatt tcgccgagcg agtactcgtc ggcccaatcg
     1801 gcgaccgctt ccatctgaac gtccgttgcg tcgcccgggg cgatggtggt cggcttcagc
     1861 gacagcgtga ccgacgaata gccggacacc ttgtgcgggc gcacgttccg ttcgacccag
     1921 cgcgcaaatg cgcggctttc cagcaaatgc ttttcgaacg atgtatccgt gtcggccagc
     1981 ttttcataca ccggcggctg gaagtattgc gacacgcgat ccacttcggc ttgcgtgagc
     2041 gtcgacggac cgtccttcag gtgttgccat tcttcttcga cctgcgccga gaatttctcc
     2101 gggctcaacg ccttcacgag aatcttgata cgcgccttgt acatattgtc gcggcggccg
     2161 tagcggttgt acacgcgcag cacggcttcg cagtacgtca gcagatgttg ccacggtaaa
     2221 tcgcggcgaa tgatcgcgcc gatgatcggc gtacggccca tgccgccgcc ggccaggatg
     2281 tcggccacca gttcgccctg ctcgttcttc ttcagataca cgcccatgtc gtggatctgc
     2341 acggcagcac ggtcttcttt cgaacccgac acggcgatct tgaacttacg cggcagccac
     2401 gcgaattccg ggtggaacgt cgaccattgg cgcaggattt ctgcccaggg tcgcggatcg
     2461 accacttcat ccggcgccac gccggcgaac tggtcagccg tgatgttgcg gatacagttg
     2521 cccgaggtct ggatgccgtg catctgcacc gcggcgagct tgcgcaggat ttccggcgtt
     2581 tcttcgagct tgatccagtt gtactggatg ttcgagcgcg tcgagaagtg gccgtagccg
     2641 cggtcgtgtt cgcgcgcgat ccgggccagc acgcgcatct ggtcgctgcg caggttgccg
     2701 tacggaatcg cgatgcggtg catgtacgcg tggcgctgca ggtacaggcc gttctgcagg
     2761 cgcagcggac gaaactcatc ttcgctcaat tcgcccgaca agcggcggcg aacctgatcg
     2821 gcgtattgct cgacccgttc atcgacgatg gtctggtcgt actgatcgta ttgatacatt
     2881 cggggacccc aatttttttg cagtgataca cggcggtccg gttccgccgc gccttggata
     2941 tcgctatttg tcaccgaccg ccgatgaacg tttagaccca ccgctcattt gcttatgaca
     3001 aatacagata tccatattga aaaagatgga cagatcgtaa taaactcgcc ttatatttca
     3061 aacgactaaa aaattctttt gatatgcgga gaggttatat atgaacctgc accaattccg
     3121 cttcgtccgc gaggctgtgc ggcagaattt taatctgacc gaagcggcca aagcactgta
     3181 tacaagccag ccgggcgtct ccaaggcgat catcgagctg gaagacgagc tgggcgtcga
     3241 gatcttcacc cggcacggca agcgcgtgcg ctcgctcacc gagcctgggc gcatcattct
     3301 tcagtcggtc gagaagattc tgcaggaggt tgagagcctc aagcgggtcg gtaaagatta
     3361 cgcggcgcag gatcagggca atctggtgat cgccgcgacg cacacccagg cacgctactc
     3421 acttccggcc gcgatcgctg aattcaagaa acgcttcccg aaagttcacc tttcgatcct
     3481 gcaaggcaac ccgactcagg tcgccgagat ggtgattcat gaccaggccg atctggcgat
     3541 cgccaccgag gcgatcgcca gctataagga actggtctcg ctgccctgct tccagtggca
     3601 ccacgtcgcc gtgatgcagc cggatcatcc gctgctcgac cgcaaacttt tgtcgttgga
     3661 tgatctgacc cagtatccgc tgattacgta cgacaacgcc ttcgccggcc gcaccaagat
     3721 caacgaggcg ttccgcctgc gcggattgca tccggacatc gtgctggagg cgatcgacgc
     3781 cgacgtgatc aagacctacg ttgaactggg tctgggcgtc ggcatcatgg cggatatcgc
     3841 gttcaatgcc gagcgtgacc ggcatctgcg cgccatgccg gtcggccacc tgttcggcag
     3901 caatgtgacg cgggtcgcgc tgaaacaggg cgcgtatctg cgcagctatg tgtatacgct
     3961 cgtcgaactg ttgtcgccga gcatgaaccg caagctgatc gaacaggccc tcaaaggcga
     4021 acacgaaacc tacgaacttt gatatcaccc ccgcttcctg ctatccccgc tacggagacc
     4081 cctagatgac gtcgtataag aaaaagatca gcggcctcac cgccgctttc ggttttggcg
     4141 cgctcctgct cgccaccgca gcccacgccg acatcaaggt cggcatcgat ctgtccagca
     4201 ccggcccggc ggccgtgatc ggcatcacca gcaagaacgc gatgctgatg tggccggcca
     4261 cgattgccgg ccagaaagcc gactacatct tcctcgacga cgcctccgac ccgggcgtgg
     4321 cggttcgcaa tatccgcaag ctgatcaacg aagaccacgt cgacgtgatc gtcggcccga
     4381 atatcacgcc ggcagccatg gccgcgctcg atcccgttgc cgaaagccag acgccgatga
     4441 tcacgctgat cggctcggcc agcgtagtgg aaccgcagga aggcaagaag atatgggcct
     4501 acaagatggc gcagaccgac agcgcgatgg ccgatgtgat gacgcgctac atgtcgaacc
     4561 acaacgtgaa gacagtgggc ttcatcggtt tcgcggacgg ttacggcgag agctggctca
     4621 acgagttcag caagttcgcg gctttgcgcc atatccagtt ggtggcgacg gagcgctata
     4681 accgcaccga cgcgagcgtg accggccaga ttctcaagct gatggccgcg aagcccgacg
     4741 caatcctgat cgccggcgcg ggcacgccga ccgtgttgcc gcagcgcacg ctgatcgagc
     4801 gcggtttcaa aggcccgatc tatcagacgc acggcatcgc cacgcccgag ttcatcaagc
     4861 ttggcggcaa ggacgtggaa ggcaccctgt tcccgaccca gccggtggtg gtcgcacgca
     4921 cgctgccggc cgatcatccg gcgaaaaaag cagcgctcgc gttcaccgac caatacgagg
     4981 cgaagtacgg cccgggcagc gtgacccaat tcgcgggcga tgcggcg
//
LOCUS       DATASH010000157         3844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4374283,
            whole genome shotgun sequence.
ACCESSION   DATASH010000157 DATASH010000000
VERSION     DATASH010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4374283"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..682
                     /locus_tag="VHV99_04645"
     CDS             56..682
                     /locus_tag="VHV99_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HEX3634765.1"
                     /translation="MGSVSMQSQASSRGERLSAFVDGELFGEEHLNTFVSELDGEDRA
                     AWSCYHLIGDALRSDDLAVSPAASSAFLSGFAARFDNEPHVLAPAAMPVARRLLALRR
                     RVVPAFAVAAAAATLTWIVVPQLQGVPGGPGVTQVAATQSHGDSLQRVAMASVPAATV
                     QPVAQDANIIRDASLDQYLEAHQQFAQQPVMPGSMSLIRAAAVSTQGQ"
     gene            687..1730
                     /locus_tag="VHV99_04650"
     CDS             687..1730
                     /locus_tag="VHV99_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HEX3634766.1"
                     /translation="MQTPRLNKTTIWGRLPAFLFCAAVLLSATPRVFAQTEDPVVARR
                     TAAELLDRIHQAAQQQNYEGAFVYQRGNFVQTSRIAHYATRTDGEFEQLESLDGKPRK
                     MLRHNDDLYTFVPERHLCVVEKRQNKDSFPALLAVSGEQVLSVYEPKLLGEDRVAGVD
                     SQVIELDPKDAYRFAYKLWADKKTGLLLRAQTLDPNGEVLEQLSFSQIRIGVPVDKTP
                     IVNGIRNTAGWTVVRPPVEPVDMAAQGWQITPTVPGFRKIRELRRPMAARDAGQPTIP
                     VDQAVFSDGLAAISVFVEPVENNTRKEGAGSSGATHVLVKRRGDFWITLLGEVPQTTL
                     QQFASAIEYKAPK"
     gene            1768..3285
                     /locus_tag="VHV99_04655"
     CDS             1768..3285
                     /locus_tag="VHV99_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEX3634767.1"
                     /translation="MTTFSVRKFLAAAVVAACLPLVPHTASAASAANLPDFTDLVDKV
                     GPAVVNIRTTTRVSNSGARGGLPPGMDDGDMSEFFRRFFGIPLPQSPQSPGAPSSPRG
                     DDNGGSGGSQDSPDNNDPEQNSGVGSGFILSADGYVMTNAHVVDDADTIYVTLTDKRE
                     FKAKLIGVDDRTDVAVVKISAANLPTITIGDSNKVRVGEWVVAIGSPFGLENTVTAGI
                     VSAKGRDTGDYLPFIQTDVAVNPGNSGGPLINMQGEVIGINSQIYSRTGGFMGISFAI
                     PIDEAMRVADQLKSSGKVVRGRIAVAIGEVTKDVADSLGLPKAQGALVSSVEPGGPAD
                     KAGVQPGDIILKFNGHSVDTATDLPRMVGDTKPGTKSTITIWRKGQTRDLPVTIAEMQ
                     PDKTAKADQKKPPVPKQRATNVLGLAVSDIPPDQLKALKLRNGVQIDAVDGPAVRVGL
                     QKGDIVLRVGDTDITSAKQFDDLTSHLDSQKMVALLVRRGENTQFVPIRPRSTQK"
     gene            3282..3572
                     /locus_tag="VHV99_04660"
     CDS             3282..3572
                     /locus_tag="VHV99_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEX3634768.1"
                     /translation="MKQVAPLTLYGRAWCHLCDDMRAALEPLLVEFSAQAVVIDIDAD
                     PLLEARYNELVPVLVCDGVELCHYHLDAPRVRAALVARLAAAHSESPHSGSS"
BASE COUNT          719 a         1157 c         1208 g          760 t
ORIGIN      
        1 tgcaatatct ggattagtgg tgtcactacg gggtatttgt aagatgggga gcatcatggg
       61 gtcggtctcg atgcaatcgc aagccagctc gcgcggcgag cgtctgtccg cttttgtcga
      121 cggtgagctg ttcggcgaag agcatctgaa cacgtttgtt tccgaactgg acggcgaaga
      181 tcgagccgcc tggtcgtgct atcacctgat cggcgacgct ctgcgttccg acgatctggc
      241 ggtcagcccg gcggcgagca gcgcattcct gagcggtttt gccgcacgtt tcgacaacga
      301 gccgcacgtg ctcgcgccgg ccgcaatgcc ggtcgcgcgc cgtttgctgg cgttgcgtcg
      361 gcgcgttgtg ccggcgtttg ctgtcgctgc cgccgcagcc acgctaacgt ggatcgtcgt
      421 gccgcaactg caaggcgtgc cgggcggccc gggtgtaaca caggtcgcgg ccacccagtc
      481 acatggcgat tcgctgcaac gcgtggccat ggcgtcggtg ccggctgcga cggtccagcc
      541 ggtcgcgcaa gacgccaaca ttattcgtga cgcaagtctc gatcaatatc tggaagctca
      601 ccagcaattc gcgcaacaac cggttatgcc gggctcgatg tcgctgattc gcgctgccgc
      661 agtttctacg caaggccaat agtttgatgc agactccgcg gttgaataaa acgactatct
      721 gggggcggct gccggcattt ctattctgcg cagccgtatt gttgtccgct acaccgcggg
      781 tctttgcaca aaccgaggat ccagtcgtcg cccgtcgcac ggccgcggag ctgctcgatc
      841 gaattcatca ggccgcccag cagcagaact acgagggcgc gtttgtctat cagcgcggca
      901 atttcgttca gacgtcgcgg atcgcgcatt acgcgactcg tacggacggc gaattcgagc
      961 agctcgaaag cctcgatggc aagccgcgca aaatgcttcg ccataacgac gatctctaca
     1021 cgttcgtgcc cgaacggcat ctgtgcgtgg tcgagaagcg ccagaacaaa gattcttttc
     1081 ctgcgttgct ggctgtaagc ggtgagcagg tgctgtctgt gtacgagccg aagctgctcg
     1141 gcgaggatcg cgtcgcgggt gtcgacagcc aggtcatcga gctcgacccg aaagacgcct
     1201 accgctttgc ttataagctg tgggccgaca agaaaaccgg cttgttgctg cgcgcgcaga
     1261 cgcttgaccc gaacggcgag gtgctcgaac agttgtcgtt ttcgcagatt cgcatcggcg
     1321 tgccggtcga caaaaccccc atcgtcaacg gtattcgtaa tacagcagga tggaccgtgg
     1381 tgcgtccgcc ggtcgagccg gtcgacatgg ctgcgcaagg ctggcagatc acgccgacgg
     1441 tgccgggctt ccgcaagatt cgtgagttac gtcgcccaat ggccgcgcgc gacgcgggcc
     1501 agccgaccat tccggtcgac caggcggtat tctccgacgg cctcgcggcc atttcggtgt
     1561 ttgtcgagcc ggtcgagaac aacacccgca aggaaggcgc gggtagcagc ggcgccacac
     1621 acgtgctggt caagcggcgc ggcgacttct ggattactct gcttggtgaa gtgccccaga
     1681 ccacattgca gcaatttgcg tctgccatag aatataaagc tccgaagtaa tcctccgaat
     1741 cactcgaatc cctcggcttg ctacgatatg acgactttct cggtgcgcaa attcctcgcg
     1801 gccgcggtgg tagcggcgtg tttgccgctc gtgccgcaca cggcgtcggc ggcttccgcc
     1861 gccaatctgc ccgacttcac tgacctcgtc gacaaggtcg gcccagcagt cgtcaacatt
     1921 cgcacgacca cccgcgtgtc gaacagcggt gcccgtggcg gattaccgcc gggcatggac
     1981 gacggcgaca tgtcggagtt ttttcgtcgc ttcttcggca ttcctttgcc acagtcgccg
     2041 caatctccgg gcgccccaag ctcgccgcgc ggtgacgata acggcgggag tggcggcagc
     2101 caggactcgc cggataacaa cgatcccgaa cagaacagcg gcgtcggctc aggcttcatt
     2161 ctgtcggcgg acggctatgt gatgaccaac gcgcacgtcg tggacgacgc ggacaccatt
     2221 tacgtcacgc tcaccgacaa gcgcgagttc aaggcgaagc tgatcggcgt ggatgatcgg
     2281 accgacgtgg ctgtcgtgaa gatcagcgct gccaatttgc cgaccatcac gattggcgac
     2341 tcgaacaagg ttcgcgtggg cgagtgggtg gtcgcgatcg gttcgccgtt cggtctcgag
     2401 aacacagtga cggccggtat cgtcagcgcc aaggggcgag atacgggcga ttatctgccg
     2461 ttcattcaga ccgacgtagc cgtcaatccg ggtaactcgg gcggtccgct gatcaacatg
     2521 caaggcgaag tgatcggcat caactcgcag atctacagcc gcacaggtgg tttcatgggc
     2581 atctcgttcg cgattccgat cgacgaggcg atgcgcgtag ccgaccagct gaaatcttcg
     2641 ggcaaggtcg tgcgtggccg gatcgcggtg gcgatcggtg aagtgacgaa agacgtggcc
     2701 gattcgctcg ggctgccgaa ggcgcagggc gcgctggtca gcagcgtcga accaggcggt
     2761 ccggcggata aggccggcgt gcagccaggc gacatcatcc tgaagttcaa cggccattcg
     2821 gtcgacacag caacggattt gccgcgcatg gtcggcgaca cgaagccggg caccaagtcg
     2881 acgattacga tctggcgcaa gggccagacg cgcgatctgc cggtcacgat tgccgagatg
     2941 caaccggaca agaccgccaa ggcggatcag aagaagccgc cggtgccgaa gcagcgcgcc
     3001 acgaacgtgc tgggccttgc cgtcagcgat attcctcctg accaactgaa ggcgctgaaa
     3061 ttgcgcaacg gcgtgcagat cgacgcggta gacgggccgg ccgtgcgcgt tgggctgcaa
     3121 aagggcgaca tcgtcctgcg tgtcggcgac acggacatca cgagcgcgaa gcagttcgac
     3181 gatctgacct cgcatctcga ctcgcaaaag atggtggcct tgctggtccg tcgcggcgag
     3241 aacacgcagt tcgtgccgat tcgcccgcgc agcacgcaga aatgaagcag gtggcgccgc
     3301 tcacgctcta cgggcgcgcg tggtgccact tatgcgatga catgcgcgcg gcgctcgagc
     3361 ctttgctggt tgagttcagt gcgcaagccg tggtgatcga catcgacgcc gatccgttgc
     3421 tggaagcgcg ttataacgaa cttgtgccgg ttctcgtctg tgacggcgtc gaattgtgtc
     3481 actatcacct cgacgcgccg cgggttcgtg ccgcacttgt cgcacgcctg gcagcggcgc
     3541 attcggaatc cccgcattcg gggtccagtt aaaggtccaa accgcgcacg aacgtgcgtt
     3601 gtcgaccttt tgtttcgcgt ctctcctgtc gacccagagt tggcgcttcg gcgcttactc
     3661 tggtcccata cacggtagct gctgagccgt cttttctgcc cgggcttcgc aagatgcgcc
     3721 gggcgatagc cttttcggct aaaatagata ggtttttcac ctacttacaa ggcgtgctcc
     3781 gctattgtcc gagcgcgcct ttttcgcttg atcggtactg aatggatcat attcgtaact
     3841 tctc
//
LOCUS       DATASH010000158         3390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5363232,
            whole genome shotgun sequence.
ACCESSION   DATASH010000158 DATASH010000000
VERSION     DATASH010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5363232"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1204
                     /locus_tag="VHV99_04665"
     CDS             <1..1204
                     /locus_tag="VHV99_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX3634769.1"
                     /translation="VDIVVATPGRLLDHMQQKTIDLSHLEILVLDEADRMLDMGFIHD
                     IKRVLAKLPPKRQNLLFSATFSDEIKTLADNLLDSPALIEVARRNTTAETIAQKIHPV
                     DRDKKRELLTHLIKQHNWFQVLVFTRTKHGANRLAEQLTKDSISALAIHGNKSQSART
                     RALAEFKDGTLQVLVATDIAARGIDIDQLPHVVNYDLPNVPEDYVHRIGRTGRAGATG
                     EAISLVCVDELQLLKDIEKLIKRPVPQEVIAGFEPDPTAKPEPILRRGQGGGGGGGGR
                     APRQGQGAPKREGGQRESGRREGAPSSAKPAQRSGQRPSNGQQQPKPQGAKPAGNGGQ
                     PRRDGQRHDGQPRAAAHEGSVAQHAPRKPQAAKPQGGNPGALLGGAKPRNDSPRGGQP
                     TRSGQRGR"
     gene            complement(1246..1770)
                     /locus_tag="VHV99_04670"
     CDS             complement(1246..1770)
                     /locus_tag="VHV99_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634770.1"
                     /translation="MSDSPAPERPLEQQVFDEWPDAVRTLFDGSSLASKTGFTASLLT
                     VDANGHLRTSLLGVGELYAPDSRALCIALWPQARAARVIAQSGRAALTFVCDEAFYQV
                     QLRFTPLPAVTGDDSGLVYLIGSIDTGEAQSVRYARLTSGITFELEREGAAVLDRWER
                     QIEHLKQAAAAASR"
     gene            complement(1901..2728)
                     /locus_tag="VHV99_04675"
     CDS             complement(1901..2728)
                     /locus_tag="VHV99_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX3634771.1"
                     /translation="MPTELNHVMPWRIEDIDLTRIDRQKAVANEDLLLLLCAASFIES
                     GTDLYTSNLSTFFDGDPEVSAWLNQEWEPEEMQHGRALKTYIAYVWPEFDWDTAFRDF
                     MEEYSLTCSVEDFEKTRALEMVARCVVETGTATLYRAIGECSDEPVLKQITDNIRTDE
                     VRHYKHFFKFFKKYNKIEGNGRLAVLGALMRRVIEIKNEDSEIALRHVFAIRYPERVH
                     DSAYNRERAARINALVRRNLSADMCVKMLLKPLDLPAKIQPGVHYPLAKITQHVFFR"
     gene            complement(3072..>3390)
                     /locus_tag="VHV99_04680"
     CDS             complement(3072..>3390)
                     /locus_tag="VHV99_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634772.1"
                     /translation="AQSTVGVYVLQLYMPMYAVKQLHMPAAASFGVVVLNGGLQFLLS
                     PVMGALSDRIGRIRIMLTTSILMGALICYYVLIAAVLSGLSLVVVAWRMRRVRHLEGV
                     QPS"
BASE COUNT          705 a         1075 c         1025 g          585 t
ORIGIN      
        1 cgtggacatc gtcgtcgcca cgccgggccg tttgctcgac catatgcagc agaagaccat
       61 tgacctgtcg catctcgaga ttctcgtgct cgacgaagcc gaccgcatgc tcgacatggg
      121 cttcatccac gacatcaagc gtgtgctcgc gaagttgccg ccgaagcgtc agaacctgct
      181 gttctcggcc accttctccg acgagatcaa gacgctcgcc gacaatctgc tcgactcgcc
      241 ggcattgatc gaagtcgccc gccgcaacac gacggctgag acgatcgcgc agaagattca
      301 cccggtcgac cgcgacaaga agcgcgagct gctcacgcac ctcatcaagc aacacaactg
      361 gttccaggtg ctggtgttca cacgcaccaa gcacggcgcg aaccgccttg ccgaacagct
      421 gaccaaagac agcatcagcg cactggcgat tcacggcaac aagagccagt cggctcgtac
      481 gcgtgcgctc gccgagttca aggacggcac gctacaagtg ctggtcgcca ctgatatcgc
      541 cgcacgcggc atcgatatcg atcagctgcc gcacgtggtc aactacgatc tgccgaacgt
      601 gccggaagat tacgtgcacc gcatcggccg cacgggccgt gcaggcgcga cgggcgaggc
      661 gatctcgctg gtgtgcgtcg acgaactgca actgctgaag gacatcgaga agctgatcaa
      721 gcgtccggtg ccgcaggaag tgatcgccgg tttcgaaccg gatccgacgg ccaagccgga
      781 gccgattctg cgccgtggtc agggtggtgg cggcggcggc ggtggacgtg cgccgcgcca
      841 gggtcaaggt gcgccaaagc gcgaaggtgg ccagcgggaa agcggccgcc gcgagggcgc
      901 gccgtcttcc gcgaagccgg cgcaacgttc gggtcagcgt ccgtcgaatg gtcagcagca
      961 gccgaagccg caaggcgcga agccggccgg caacggcggc cagccgcgcc gcgacggcca
     1021 gcgtcatgac ggtcagccgc gtgcagcggc gcacgaaggc agcgtcgcgc aacatgcgcc
     1081 gcgcaagccg caggctgcga agccgcaagg cggcaatcca ggcgctttgc tgggtggcgc
     1141 aaaaccgcgc aacgactcgc cgcgtggcgg ccaaccgaca cgcagcggcc agcgcggccg
     1201 ttaagcttta agcgctgcct gctcgccagt tgtagtccca gcagactaac ggctagccgc
     1261 agcggcagct tgcttcaggt gctcgatctg ccgttcccat cgatcgagca ccgccgcccc
     1321 ttccctttcc agttcaaacg taatgccgct tgtgaggcgc gcataacgca cgctttgcgc
     1381 ctcgcctgtg tcgatcgagc cgatgaggta gacgaggccg ctatcgtcgc ctgtcacggc
     1441 tggtagtggc gtgaaacgca actgcacctg atagaacgcc tcgtcgcaga cgaacgtcag
     1501 cgcggcccgg ccgctttgcg cgatcacgcg cgccgcccga gcctgcggcc acaacgcaat
     1561 gcatagcgcg cgtgaatcgg gcgcgtacag ctcgccgacg ccgagcaacg acgtgcgcag
     1621 atggccgttc gcatcgacgg tgagcagcga cgcggtaaat ccggtcttgc tcgccaacga
     1681 cgacccgtcg aataacgtgc ggacggcgtc gggccattcg tcgaacacct gttgttcaag
     1741 cggacgttcg ggtgcagggg agtcgctcat cgtctcgatc cggtcagtag ctgttggcga
     1801 aagtcgcaga cctcaatcat gcctcgaaaa agcgaatggc ccgcacgagc gggccattcg
     1861 ctttcgtctc tatacgcggc gcggactcag cagaaaaaca tcagcggaag aacacgtgct
     1921 gcgtgatctt ggcgagcggg taatgcacgc ccggctgaat cttggccggc agatcgagcg
     1981 gcttgagcag catcttcacg cacatatccg ccgacagatt gcgccgcacc aacgcattga
     2041 tgcgcgccgc acgctcgcgg ttataggcag aatcgtgcac gcgctccgga taacgaatcg
     2101 cgaagacatg gcgcaatgca atctccgagt cttcgttctt gatttctatc acgcggcgca
     2161 tcaatgcgcc gagaacagcc agacgtccgt tgccttcgat cttgttgtac ttcttgaaga
     2221 acttgaaaaa gtgcttgtag tgacggactt catccgtgcg gatgttgtca gtgatctgct
     2281 tgagcaccgg ctcgtccgaa cattcgccga tcgcgcgata cagcgtggcg gtgccggttt
     2341 ccaccacgca gcgcgcgacc atctcgagcg cacgggtctt ttcgaagtct tcgaccgaac
     2401 aggtcagcga atactcctcc atgaaatcgc ggaacgcggt gtcccaatcg aactcaggcc
     2461 agacgtaggc gatatacgtc ttaagcgccc ggccgtgctg catttcttcc ggctcccatt
     2521 cctggttgag ccaggctgag acttccgggt cgccgtcgaa gaaggtgctc agattgctgg
     2581 tgtaaagatc ggtaccgctt tcgataaacg aggcggcaca caacagaagc agcaggtctt
     2641 cgttagcgac ggccttctgg cgatctatgc gggtgaggtc gatatcctcg atccgccagg
     2701 gcatcacatg gttcagctca gtgggcattg tgtctttgct ccgaaagctg tatcgactcg
     2761 tggagcgcgc tcaaaaccgc ccgctatcgc aagggcggtc cgccgtgtca gatacagcgc
     2821 cttgaattag agttaacccc gtatatctgc atcttagccg gtgtttccca actctgcagg
     2881 taaccctgca cagaccgtgc cgccctgccg cagttccaat ttcgcgcgaa ttgaatcctt
     2941 cggttgatta caaaccgcgc aagcgggcca taaaaaaagc cagctcgatg agctggcttt
     3001 ggcttgaaca gagggtgcag gtcaagcgca gcaaggttcg ctgcgtggca cagcgaccgc
     3061 ccgcaacgcc gtcatgaggg ttgaacgcct tcgagatgcc ggacgcgccg catccgccac
     3121 gcgaccacga caagcgacaa cccggacaac actgcggcaa tcaggacgta gtaacagatc
     3181 agcgcgccca tcagaatcga cgtggtcagc atgatgcgaa tccggccgat gcggtcggac
     3241 aacgcgccca tcaccggcga gagcagaaac tgcaagccgc cgttgagcac caccacgccg
     3301 aaagacgccg ccgccggcat gtgcaactgt ttgaccgcgt acatcggcat gtagagttgc
     3361 agcacataca cgccgaccgt cgattgcgcg
//
LOCUS       DATASH010000159         4354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3582233,
            whole genome shotgun sequence.
ACCESSION   DATASH010000159 DATASH010000000
VERSION     DATASH010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3582233"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1713)
                     /locus_tag="VHV99_04685"
     CDS             complement(<1..1713)
                     /locus_tag="VHV99_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014696821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEX3634773.1"
                     /translation="MQSPVQKRIAAAAVLTILLTIAGCASTGGIAPQDRGIEPASLDA
                     GNAIRAANADAQWPATDWWRAYRDPQLNEWIEAAQTGNPSLAMAQARVREAVSMAGVA
                     RSALSPQVNGSLSIQRQKWADNLYYGPGPLAGEQSWNNTGTLALSYNLDLWGKDKNAA
                     EGALDAAHASAADFRAAQLELEGNVVRTYIEMSMNYALLDIAKSTLQQQQQIVDLANR
                     RLKGGIGTQLEVSQAETPLPEYERQIDEIEEKIALGRNQLAALAGKGPGAGDAIQRPT
                     LSLDSAPAGLPSALPADLIGHRPDVVAARWTVATQARGIDVAKASFYPDINLLASIGG
                     YAAMGPLFQFLKDPSHSWSAGPALSLPILDGGRLRSQLGAASAGYDEAVDRYNQSIVG
                     ALKDISDQVIRIRSLATQAQDADRSVAAARKNYDLAREGYRRGLTDYLNVLIAQSQLL
                     HAQEGVAKIQAERLGAHASLVTALGGGLDEPANGPQANETLPAHGKGKAVAAGSTAAA
                     KSSNAPTQTDSASHQGSTAPAAATAPAQSPAASAAKPSRAAAKRASDTGMSAAATTSH
                     LSSTA"
     gene            1869..2807
                     /locus_tag="VHV99_04690"
     CDS             1869..2807
                     /locus_tag="VHV99_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3634774.1"
                     /translation="MDTLQNMRVFVRVVEAGSFTGAAQHLNTTTAYASRAVSDLEAHL
                     RTRLLNRTTRRIALTEAGERYLQRCEQILAYVDQAEAEAGDAHARPSGKLKVHAMTSF
                     GQHYVVPAVGRYQARYPDVHVELTLAQRMPDLLDEGFDVSLTLATGLPDSGLVSQRLG
                     SAFSIVCASPAYLERHGVPQTPADLARHTCLQMVTPIFPTDKWIFDGPNGEETIALGP
                     ATFQVNVAEAMAVAASSGMGVGLIPIYSAISGLRSGELVWLLPEYTSQEMNLYALYPS
                     RQYLDAKIRTWVEFLRDELPATLAVDQEELRQFARS"
     gene            3044..3202
                     /locus_tag="VHV99_04695"
     CDS             3044..3202
                     /locus_tag="VHV99_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634775.1"
                     /translation="MDTHLMIGVAVMLGLIGIAASRELLRRLREQQPQLMPIKVERPD
                     LRGQRHER"
     gene            complement(3433..4152)
                     /locus_tag="VHV99_04700"
     CDS             complement(3433..4152)
                     /locus_tag="VHV99_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEX3634776.1"
                     /translation="MCGRISQYRDPRLYAQQLGLADPLMLFDAADRRPGYNLAPGTHP
                     LAIYPDQTLRAVHWGYCPDWAREQHLPQTINARAETAPHTRYFKDLWKTGRVLVPADG
                     WFEWRMEAVSAPSNGATAAQAEATVRQPYFVHLKSAVPMYLAALSSVRGTQAQTDGMG
                     LVIVTATADAGLIDVHDRRPLVFAPEAARRWLDAALQFDELEQLARSACLPAARFRWH
                     RVSPEVDRTLNDEPGLIEALH"
     gene            complement(4238..>4354)
                     /locus_tag="VHV99_04705"
     CDS             complement(4238..>4354)
                     /locus_tag="VHV99_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX3634777.1"
                     /translation="FDYTVNGTRVVCNPRGYFDRRTGQWENPQFTWDKVVEI"
BASE COUNT          716 a         1355 c         1415 g          868 t
ORIGIN      
        1 tgcggtggag gacaggtgag acgtggtggc cgcggcgctc ataccggtgt cgctcgcccg
       61 tttggcggca gcgcgcgatg gctttgccgc agaggcggcc gggctttgcg cgggtgccgt
      121 ggccgcagcg ggtgcggtgc tgccctgatg actcgccgaa tcggtctgcg tcggcgcgtt
      181 cgacgactta gccgcagcgg tcgaacctgc cgccaccgct ttgcccttgc catgcgccgg
      241 caacgtctcg tttgcctgtg gtccgttcgc cggttcgtcg agcccaccgc ccaacgccgt
      301 gaccagcgac gcatgcgcgc cgaggcgctc ggcctgaatt ttcgcgacgc cttcctgcgc
      361 gtgcagcaat tggctttgcg cgatcagcac attcagatag tcggtcaggc cgcgtcgata
      421 cccttcacgc gcgaggtcgt agttcttgcg agccgccgcg accgaacggt cggcgtcttg
      481 cgcctgggtt gcgagcgaac ggatccggat cacctgatcg gagatgtcct tcagcgcgcc
      541 gacgatcgac tggttgtagc gatcgaccgc ctcgtcgtac cctgccgacg ccgcgccgag
      601 ttgcgagcgc aagcgcccgc cgtcgaggat cggcagcgac agcgcgggac ccgcgctcca
      661 gctatgcgac gggtctttca ggaactggaa cagcggcccc atcgcggcat atccgccgat
      721 cgacgccagc aggttgatat ccggatagaa gctggccttg gcgacgtcga tgccgcgcgc
      781 ttgcgttgct accgtccagc gcgccgcgac cacgtccggt cgatggccga tcagatccgc
      841 gggcagtgcg ctcggcagac cggcgggtgc gctgtcgagc gagagcgtcg gacgctggat
      901 tgcgtcaccc gcgccaggcc ccttgccggc cagtgcggcc aattgattgc ggccgagtgc
      961 gatcttttcc tcgatctcat cgatctgccg ttcgtattcc ggcagcggcg tttcggcctg
     1021 gctcacttcg agttgcgtgc cgatgccgcc tttcagacgc cggttcgcca ggtcgacgat
     1081 ctgctgctgt tgctgcagcg tggatttggc gatatcgagt agcgcgtagt tcatcgacat
     1141 ctcgatgtac gtgcgcacca cgttgccttc cagttcgagc tgggcggcgc ggaagtccgc
     1201 cgcgctggcg tgagcggcgt cgagcgcgcc ttcggcggcg ttcttgtcct tgccccagag
     1261 atcgaggttg tacgacagag ccagcgtgcc ggtgttattc cacgattgtt cgcccgcgag
     1321 ggggcccggg ccgtaataga ggttgtcggc ccatttctgg cgctggatcg acagactgcc
     1381 gttcacttgc ggcgacagcg ccgaacgggc gacgcccgcc atcgacacgg cttcccgcac
     1441 acgcgcctgg gccatagcta ggctcggatt gcccgtctgc gcggcttcga tccactcatt
     1501 gagttgcgga tcgcgatagg cacgccacca gtcggtggct ggccattggg cgtccgcgtt
     1561 ggcggcgcgg atggcgttgc ctgcgtcgag cgaggcgggc tcgattccgc ggtcttgggg
     1621 cgcaatgcct ccggtactgg cacagccggc gattgttaat aggatcgtaa gaaccgcagc
     1681 tgcggcgatc cgtttctgta ccggagactg cacgatttcc tccaaaattg gcaatagaag
     1741 catgtgcgtc attatatttt tcgattgatt ggggaataac cggtaaagat gcaaggcatt
     1801 tttacgcgat atgagataat cgcctttgcg aatcgtgcaa caatcgctac cgttctcttg
     1861 ggaaaagtat ggatacgctt caaaacatgc gcgtatttgt ccgcgtcgtc gaagcgggca
     1921 gctttacggg tgccgcgcag cacctgaaca cgaccacggc gtatgcgtcg cgcgcggttt
     1981 ccgacctgga agcgcatctg cgcacgcgtc tgttgaatcg caccacgcgc cgtattgcgt
     2041 tgaccgaggc gggtgagcgc tatctgcagc gctgcgagca gatcctcgcg tacgtggatc
     2101 aggccgaagc ggaagccggc gacgcccacg cacgtccatc cggcaagctc aaggtccacg
     2161 cgatgacgag cttcggccag cactacgtgg tgccggcggt gggccgttat caggcgcgct
     2221 atccagatgt gcatgtcgaa ctgacgctcg cgcagcgcat gcccgatctg ctcgatgaag
     2281 gtttcgatgt ctcgctgacc ctcgcgaccg gcctgccgga ttcggggctg gtgtcgcagc
     2341 ggctgggcag cgcgttcagc atcgtgtgtg catcgccggc ttatctggag cggcacggcg
     2401 tgccccaaac gcccgcggac ctcgcgcgcc acacttgcct gcaaatggtc acgccgattt
     2461 ttccgaccga caaatggatt ttcgacggcc cgaacggcga ggaaacgatt gccctgggcc
     2521 cggccacctt ccaggtgaat gtcgccgaag cgatggctgt cgcggcatcc agtggcatgg
     2581 gcgtgggcct gattccgatc tactcggcga tcagcggttt gcgtagcggt gagttggtgt
     2641 ggcttttgcc ggaatacacg tcgcaggaaa tgaatctgta cgcgctgtat ccgtcacggc
     2701 agtatctgga cgcgaaaatt cgcacctggg tcgaatttct gcgcgacgaa ctgcctgcca
     2761 cgttggctgt cgatcaggaa gaattgcgcc agttcgcgcg cagctgaagc agcgccgagc
     2821 gagcggttga ccactgtcga cggctttggc cagccatggc atgacagcct gttacataag
     2881 tgccgcgctg caacatttcc ttacttccgc gccgcgatcg atttgctaac gtgtggttca
     2941 tacgctgccg cattcagccg gacgcgttca ataagccgct gttcggcaaa tcgcaagaac
     3001 cgcaacaaac gaacaatcgc aagaactcaa ggacgaggaa ctcatggata cgcatctgat
     3061 gatcggcgtg gccgtcatgt tgggactgat cggaatcgcc gcgtcgcgcg aactgttgcg
     3121 ccgtctgcgt gaacagcagc cgcaactgat gccgatcaag gtcgagcgtc ctgatctgcg
     3181 gggacagcgg cacgagcgtt gaagcttgag agcgtgccgg attgcccggc gcgcggtgat
     3241 tgagcttgct gttgcgtttt tccggcgtgt tttccgcgtt accgcgccat tgcggtctgg
     3301 ctgccgatct gtttcgatcc gcgctccata tgcgtggttt ggtgcttcga tccgcagtcc
     3361 atacggttga tccagtgttt cgatccgcgt tccgcgcggg tggtgcagcg cttcgactcg
     3421 cgctccgcac gcctagtgca gcgcttcaat cagacccggc tcatcattga gcgtgcggtc
     3481 cacctccgga ctcacccgat gccagcgaaa ccgcgcggcg ggcaggcagg cgctgcgcgc
     3541 aagctgttcg agttcgtcga actgcaaagc cgcatcgagc cagcggcgcg ccgcttcggg
     3601 cgcaaatacc agcgggcggc ggtcgtgcac gtcgatcagg cccgcgtcgg ccgtggccgt
     3661 cacgatcacc aatcccatgc cgtccgtttg cgcttgcgtg ccgcgtacgc tcgataaggc
     3721 cgcgaggtac atcgggacgg cgcttttaag atgcacgaag tagggctggc ggactgtggc
     3781 ctcggcttgt gcggcggtgg cgccgtttga tggtgcggac accgcttcca tgcgccattc
     3841 gaaccagccg tctgccggca ccagtacccg tcccgttttc cacaggtctt tgaagtagcg
     3901 cgtgtgcggc gcggtttcgg cgcgcgcgtt gatcgtctgc ggcagatgct gctcgcgtgc
     3961 ccaatccggg caataccccc agtggaccgc acgcaacgtc tggtccgggt aaatcgcgag
     4021 cggatgggtg cccggcgcga ggttgtaccc ggggcggcgg tctgcggcgt cgaacagcat
     4081 gagcggatcg gcgaggccga gctgctgggc gtacagacgc ggatcgcggt actggctgat
     4141 tcgaccacac atgagcaacc tcctggcaag acccgcgaca acaacagcgt agtgcgtcgc
     4201 gcctggccgc gctacccaga tcggcccgaa gccgatgcta tatctccacg accttgtccc
     4261 acgtgaactg cggattctcc cactgcccgg tgcgccggtc gaaatagccg cgtggattgc
     4321 agaccacgcg cgtgccgttc acggtgtaat cgaa
//
LOCUS       DATASH010000160         3654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4176661,
            whole genome shotgun sequence.
ACCESSION   DATASH010000160 DATASH010000000
VERSION     DATASH010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3654
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4176661"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VHV99_04710"
     CDS             <1..322
                     /locus_tag="VHV99_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation efflux family transporter"
                     /protein_id="HEX3634778.1"
                     /translation="GVGVLLMVIAWLVAREVKSMIVGESASPDVRRAIEAHLRARAEI
                     RSIINMITLQWGRHVVVAVQAEMVDYASGRAMVDAINVIEADLQAAFPQVRWVFFEPD
                     VPRG"
     gene            complement(426..1406)
                     /locus_tag="VHV99_04715"
     CDS             complement(426..1406)
                     /locus_tag="VHV99_04715"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX3634779.1"
                     /translation="MDYVKLGRTGLDVSRLCLGCMSYGVPARGTHPWSLDDEAARPFI
                     KQALDHGINFFDTANVYSDGTSEEIVGRALKDFAKRDEIVLATKVHGRMRPGQNGAGL
                     SRKAIMAEIDHSLRRLGTDYVDLYQIHRWDYGTPIEETMEALHDVVKAGKARYIGASS
                     MYAWQFAKALHVAESNGWTRFVTMQNYVNLLYREEEREMLPLCESEGIGVIPWSPLAR
                     GRLTRDWNTESARSETDEFSRTLYAHTEDADRRIVERVSQIAKERGVPRAQVALAWVL
                     QKKPITAPIVGATKLHHLDDAVAALSLNLSADEIRQLEELYVPHAVAGFK"
     gene            1691..2764
                     /locus_tag="VHV99_04720"
     CDS             1691..2764
                     /locus_tag="VHV99_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside permease"
                     /protein_id="HEX3634780.1"
                     /translation="MLTRALGAICATTLAACAAFTPGPASADDNDGFSRGDGGSHGRP
                     VKVMIISMFGPEGQVWLDRLGPWREIAVDGLSPDYPTVHCNKQEVCVMTTGMGHTNAA
                     ASIMALTFSPRFDLRHTYFMVAGIAGIDPQQGTVGSAAWAKYLVDFGIQWEIDGREIP
                     AGWNTGYLGINTKSPTDKPPLDYRTEVFQLNTQLADTAFALSRSVVLSDDAQAQAARA
                     RYSYAPANRPPAVIQCDTLAGDTWWSGTLLGQRARDWTKILTDNKGVYCTTQQEDNAT
                     YEALKRAASVHRVDLNRVAVLRAGSDFDRPYDGQTSADNLLNYAAQGGFTIAIENLFR
                     SGNPVVQDIVSHWGEWREGVPKR"
     gene            complement(2754..3398)
                     /locus_tag="VHV99_04725"
     CDS             complement(2754..3398)
                     /locus_tag="VHV99_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634781.1"
                     /translation="MFSSSGFIAPWPGVYQLFASSAFLLFCFFDFLLCCFSALRLSGS
                     PALRLSGFSALRLFGFSAFRLFGFFALLLPLVLPRRSQWFPRANEGSGFRGQPLRDAR
                     SAEWELMSPWSLTPNVRGHGFQMHHYRDCAGDPLMSWRFEPLSFAYFSLRQAKKSRCP
                     PRTGATLIDQYQFKERPTPQADKQTSTARQPDQQKANTAGTPTKRRTGKNPFTF"
BASE COUNT          696 a          982 c         1196 g          780 t
ORIGIN      
        1 cggtgtgggt gtgttgctga tggtgattgc gtggctggtg gcgcgcgagg tgaagtcgat
       61 gatcgtcggc gaatcggcga gcccggacgt gcgccgcgcg atcgaggcgc atctgcgcgc
      121 gcgcgccgag attcgcagca tcatcaacat gattacgctg cagtggggtc gtcacgtggt
      181 ggtggcggtg caggcggaaa tggtcgacta cgcgagcggc cgcgcgatgg tcgatgcaat
      241 caatgtgatc gaagcggact tgcaggcggc gtttccacaa gtgcgctggg tgttttttga
      301 accggacgta ccgcggggtt gaagggttat ctcgctgcga ggcacgtccg atacattgag
      361 ggccggcgga tttcgcacag gtctgcgttc agacttttgc gtccgccggc tggtgcattt
      421 gccgcttatt tgaagccggc cactgcatgt ggcacataca gttcttccag ttgacggatt
      481 tcgtccgcgc tcagattcaa cgacagtgcc gcgaccgcat cgtccagatg atgcagtttg
      541 gtcgcgccga cgatcggcgc ggtgatcggc ttcttctgca acacccaggc tagcgcgact
      601 tgcgcacgcg gcacgccgcg ctccttggca atctgactga cccgttcgac gattcgccta
      661 tccgcgtctt cggtatgcgc gtataacgtg cggctgaatt cgtcggtctc cgagcgtgcg
      721 ctttcggtgt tccagtcgcg cgtcaggcgt ccgcgcgcca gcgggctcca gggaatcaca
      781 ccgatgcctt cgctttcgca cagcggcagc atctcgcgct cttcttcgcg gtacagcagg
      841 ttcacgtagt tctgcattgt cacgaagcgc gtccagccat tgctttcggc cacatgcaat
      901 gccttggcga actgccatgc atacatcgac gatgcgccga tatagcgtgc cttgccggcc
      961 ttcacgacgt cgtgcagggc ttccatggtt tcttcgatcg gcgtgccgta gtcccagcgg
     1021 tggatctgat acagatcgac gtaatccgtc ccgaggcgcc tcaggctgtg gtcgatttcc
     1081 gccatgatcg ccttgcgcga cagacctgcg ccgttctgac ccggacgcat gcggccatgg
     1141 accttggtag ccagcacgat ttcgtcgcgc ttcgcaaagt ccttcagcgc gcggccgacg
     1201 atttcctcgc tggtgccgtc tgagtaaacg ttggcggtat cgaagaaatt gatgccgtga
     1261 tcgagcgcct gcttgatgaa gggacgcgcc gcttcatcgt cgagcgacca ggggtgggta
     1321 ccgcgagcgg gcacgccgta gctcatacag ccgagacaaa gacgcgatac atcgaggccg
     1381 gtgcggccga gtttcacata atccatcgtg gtgctcctgg gttgaagtgc gcacacgcgg
     1441 tgcgtgctga gggcctccat tataggaagg gtggtgccga cgtgctggcg gcccgacagt
     1501 tgaccaggaa gggccgcgtc gacgtgctgg cggcccgcca ggccgaccga acgcgcaggc
     1561 tgacggggtt caatcgcggc gggatgcggc aacgcgtagt ggcaaataat ttcttcgcac
     1621 ctatacttct ttcggcttga aagcagcacc ttctcccaat ccgcatcaca ggcaaaaaga
     1681 aaggatcacg atgctgactc gcgcacttgg cgcaatctgc gccactacac tcgcggcttg
     1741 cgccgctttc acacccggcc ccgccagcgc ggacgacaac gacggtttct cgcgcggcga
     1801 tggcggctcg cacgggcggc cggtcaaggt gatgatcatt tcgatgtttg ggccggaggg
     1861 gcaggtgtgg ctcgatcggt tggggccatg gcgtgagatt gctgtcgacg gcctgtcgcc
     1921 cgactacccg acggttcatt gcaacaagca ggaggtgtgt gtcatgacca cgggcatggg
     1981 gcataccaat gctgccgcgt cgatcatggc gttgaccttc tcgccgcgct tcgatttgcg
     2041 gcatacgtat tttatggttg cggggattgc cgggatcgat ccgcaacagg gcacggttgg
     2101 atccgccgcg tgggcgaaat atctggtcga ctttggcatt cagtgggaga tcgatgggcg
     2161 ggagattccg gctggctgga ataccgggta tctcgggatc aataccaaga gtccaacgga
     2221 caagccgccg cttgattatc ggactgaggt gtttcagttg aatacccagc ttgccgatac
     2281 ggcgtttgca ttgtcgcgta gcgtggtgct ttccgatgac gctcaggctc aggctgcgcg
     2341 ggccaggtat tcttatgcgc cggcgaatcg gccgcctgct gtgattcaat gcgatacgct
     2401 ggccggggac acctggtggt cagggacgct gcttgggcag cgggctcgcg actggacgaa
     2461 gattttgact gacaacaagg gggtttattg cactacgcag caggaggata acgccacgta
     2521 tgaggcgctt aagcgcgcgg cctcggtgca tagggtcgat ctgaatcgcg ttgcggtgct
     2581 gcgggctggg tcggattttg atcggcctta tgatgggcag actagtgcgg ataatttgct
     2641 gaactatgct gcgcaagggg gttttacgat cgctattgag aatttgttta ggagtgggaa
     2701 tccggttgtg caggatattg ttagtcattg gggggagtgg cgggaggggg tgcctaaaag
     2761 gtgaatgggt ttttgcctgt gcggcgcttt gttggtgtgc ctgcggtgtt ggctttttgt
     2821 tgatctggtt gccgtgcggt gcttgtttgt ttgtctgcct gcggcgttgg cctttccttg
     2881 aattggtatt ggtctattag cgtcgcccct gtgcggggcg ggcacctact tttctttgcc
     2941 tgccgcaaag aaaagtaggc aaaagaaagc ggctcaaacc gccagctcat aagcgggtcc
     3001 cccgcacagt cgcggtagtg gtgcatctgg aatccgtgcc ctcgcacatt cggcgttagt
     3061 gaccaagggc tcatcagctc ccactccgca ctgcgtgcgt cgcggagggg ttggcctcgg
     3121 aaaccagagc cttcgtttgc gcgtgggaac cattggcttc gcctcggcaa aaccaatggc
     3181 aacagcagcg cgaaaaagcc gaaaagccga aaagccgaaa agccgaaaag ccggagagcc
     3241 gaaaagccgg agagccggag agccggagag ccggagagcc ggagagcaga aaagcagcaa
     3301 agcagaaaat caaaaaagca gaaaagcaga aaagcagaag aggcgaaaag ctgataaacg
     3361 ccgggccacg gcgcgatgaa gcctgaggat gaaaacatgg cggtgggctt taagaagcga
     3421 gcttcggcgc gcgtagcgcc gccggaagta tgactgcctt gtcactaggg ctaagtgtgc
     3481 gagaacaccg attccagatg cgccactatc gtgactgtgc gggggacccg cttatgcgct
     3541 ggcggtttga gccgctttct tttgcctact tttctttgcg gcaggcaaag aaaagtaggt
     3601 gcctgccccg cacaggggcg acgctaatag accaatatca attcaaggaa aggc
//
LOCUS       DATASH010000161         5543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2791393,
            whole genome shotgun sequence.
ACCESSION   DATASH010000161 DATASH010000000
VERSION     DATASH010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5543
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2791393"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="VHV99_04730"
     CDS             complement(<1..649)
                     /locus_tag="VHV99_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008924254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX3634782.1"
                     /translation="MDKTTNGWVSGMVGVLIFSGSLPATRIAVQGLDPVFLTVARATI
                     AGTLGLLLLLVFREARPARRDLIPLVVVALGVVVGFPLLTALALRHVNAAHAVVFVGL
                     LPLSTAIFGVLRGGERPQPAFWLFSALGSGAVVAFALRRGLDASPVGDTLMLAAIIVC
                     GLGYAEGARLSRHLGGWQVISWALVLSLPVMLPLAWWTRPASFEGVSHAALWGLAY"
     gene            764..2203
                     /locus_tag="VHV99_04735"
     CDS             764..2203
                     /locus_tag="VHV99_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008924255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX3634783.1"
                     /translation="MAESDNNRAAAGAQTGTRVGTVMDRLRERIASRSLMPGARVPSI
                     RMMTETLGVSKSTVVEGYDRLVAEGVIVARRGSGFFVSGHAPPLALADLGPRLDREVD
                     PLWLTRQSLETGAKGFKPGCGWMPSSWLPEDGVRRALRAVARDRQVSLAEYASPLGLP
                     ALRQQLAWRLAQHGVEAPPEQIVLTDGGTHALDLVCRFLLEPGDTVLIDDPCYFNFQA
                     LLRAHRARIVGVPYTPSGPDLAAFERALIEHRPRLYVTNSALHNPTGATLAPAVAHRL
                     LKLAGEHNLLIVEDDIFADFEDEPAPRLAAFDGLARVVSIGSFSKTLSAAVRCGYIAA
                     RSEWVEPLVDLKLATSFGNGQLAASVVHRLLVDGTYRRHLESMRAKLADAMGETIRRL
                     GYAGLEVWTRPRAGIFVWARLPDALDAADIARHALAESVVFAPGNVFSASQSAAGFLR
                     FNVSQCNRPKVYEALQRAMDVSAAARERA"
     gene            complement(2246..3319)
                     /locus_tag="VHV99_04740"
     CDS             complement(2246..3319)
                     /locus_tag="VHV99_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX3634784.1"
                     /translation="MLKRLLAGWLGLLLMVMASFAGAEPAHYKIVTGPERGTYIQIGQ
                     DLSKWVAQPVGIDLEVVASKGSAENVQRMRFEPGVKLALVQSDVYQAYIDMANAGNAD
                     AGTAIRPLRLIMPLYDEEINVVVRADSPMKTFADIKDKSISVGLLGSGTAQSATTLYH
                     LMFGQSIPEQNIQHLSNEDALAALIVKKVDVAIIVVGQPAKLFNDMNPELLQQIRPLR
                     ADPNAPETVRAKQTYFPATLRTTSYPTWLKEDVPTLTVKAFLVTYDYGLRDTVGNLNH
                     FADSLCANFDNLQEHGHPKWKQVKLELPTLVRGWKYYGPVEKHLRACLANKSAAISAT
                     ATQAVQKPRPPCTDQERLLLLCK"
     gene            complement(3526..4644)
                     /locus_tag="VHV99_04745"
     CDS             complement(3526..4644)
                     /locus_tag="VHV99_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634785.1"
                     /translation="MSTQTLLGHSFPTPCKRCGGALYRQVDYCPYCGAVHPLDTGPHK
                     RAVIPGSRASATNRTAQKNSFDPTLPDETGPAAQTAFTSDAAPAELPTQAPVLASPDA
                     PIPPLADPPDSTRRGVISMRRVLIAIAAIGAIGLAYVAYALFSDNQESQSGNTEQSAD
                     NLRDARTATGTIAPYAPAQSTNQAAVGKPVAVPTTPMNAVNPVSPVSPVSPISPISPA
                     NPVKAAPAIPASPIAPPVAAAPVKPAPQFRDAAQALQTARLAFRANDLSTAQAALGAA
                     QSLQPGDADAQSLLTELKPLTARRDAALQAAQVCVAQQSWSCARQRANEALTIDTGNE
                     TAKTILARVIRETGWAPLKPHAATDNPSGDKSFGYRIS"
     gene            complement(5143..>5543)
                     /locus_tag="VHV99_04750"
     CDS             complement(5143..>5543)
                     /locus_tag="VHV99_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EmrB/QacA family drug resistance transporter"
                     /protein_id="HEX3634786.1"
                     /translation="YIDMGILGFGLGMVMQVLILAVQNTVEFKHMGVATSGATLFRSI
                     GGSIGVAGFGAVFSNGLAGRLEKLIPPDTDLPHALGPAAIHQLPQALRDDYLQAFAGA
                     LHTVYLSAACVVVLACVLAWLLKDHPLRKH"
BASE COUNT         1020 a         1701 c         1816 g         1006 t
ORIGIN      
        1 catacgcgag cccccacagc gccgcgtggc tcacaccttc aaacgatgcg ggccgcgtcc
       61 accaggcgag cggcagcatg accggcaacg acagcactag cgcccacgaa attacctgcc
      121 agccgccgag atggcgcgac agccgcgcgc cttcggcata acccaggccg cagacgatga
      181 tcgccgccag catcagcgta tcgccgaccg gtgacgcatc gaggccgcgg cgcaaggcga
      241 acgcgaccac cgcgccgcta ccgagcgccg agaacagcca gaacgccggc tgcggacgct
      301 cgccgccgcg cagcacgccg aagatcgcgg tggacagcgg caggagcccg acgaatacca
      361 cggcatgcgc ggcattcacg tggcgcaatg caagggcggt caacagcgga aagccgacca
      421 ccacgccgag cgcgaccacg acgagcggga tgagatcgcg ccgcgcgggc cgcgcctcgc
      481 gaaacacgag cagcagcaac agccccaacg tgcctgcgat ggtcgcgcgg gccaccgtca
      541 gaaacaccgg gtcgaggcct tgtacggcga tgcgcgtggc gggtagcgag ccgctgaaaa
      601 tcaacacgcc caccatgccg ctcacccagc cgttcgttgt cttgtccatc gaagagtcca
      661 tgcacattga aatggaacgc ctagcatccg ctttcgggct gcaaagggta agctacggat
      721 cagtacaatt cacccaaact gtaccgaaca aggagcagta cagatggcgg aaagcgacaa
      781 caaccgggct gctgcgggcg cgcaaaccgg cacgcgggtc ggcaccgtga tggaccgctt
      841 gcgcgagcgc attgcgagcc gttcgctgat gccgggcgcg cgcgtgccgt cgatccgcat
      901 gatgaccgag acgctcggtg tgtcgaagtc gacggtagtg gaaggatacg accggcttgt
      961 cgccgaaggt gtgatcgtgg cgcggcgcgg ctccggtttt ttcgtgtcgg gccatgcgcc
     1021 gccgctcgcg ctcgccgatc tcgggccgcg cctcgatcgc gaggtcgacc cgctgtggct
     1081 cacgcggcag tcgctggaga cgggcgcgaa ggggttcaag ccgggctgcg gctggatgcc
     1141 gtcttcgtgg ctgccggaag acggcgtgcg ccgcgcgctg cgcgccgtgg cgcgcgaccg
     1201 ccaagtgtcg cttgccgaat acgcgagccc gctcggtctg cccgcgctgc gccagcaact
     1261 cgcgtggcgg ctcgcgcagc acggcgtgga agcgccgccc gagcagatcg tgctgaccga
     1321 cggcggcacg catgcgctcg atctggtctg ccgttttctg ctcgaacccg gcgacacggt
     1381 cctgatcgac gatccctgct acttcaactt tcaggcgttg ctgcgcgcgc atcgcgcccg
     1441 catcgtggga gtgccctata caccgtccgg gcccgatctc gcggccttcg agcgggcgct
     1501 gatcgagcat cgcccgcggc tctacgtgac caattccgcg ctccacaatc cgaccggcgc
     1561 cacgcttgcg ccggccgttg cgcatcggct gctgaaactg gcgggcgagc acaatctgct
     1621 gatcgtcgag gacgatatct tcgccgactt cgaagacgag ccggcgccgc ggctcgcggc
     1681 gttcgacggt ctcgcgcgag tggtttcgat cggcagcttt tcgaagacct tgtcggcggc
     1741 cgtgcgctgc ggctacatcg cggcgcgcag cgaatgggtc gaaccgctgg tcgatctgaa
     1801 gctggccacc tcgttcggca acgggcagct ggcggcgagt gtcgtgcatc gtttgctggt
     1861 ggacggcacc tatcggcggc atctggagtc gatgcgcgcg aagctggcgg acgccatggg
     1921 cgagacgatc aggcggctgg gctatgcggg tcttgaagta tggacgcggc cacgcgccgg
     1981 tattttcgtg tgggcgcgcc tgccggatgc gctcgacgcc gccgatatcg cgcgtcacgc
     2041 gttagccgaa agcgtggtgt tcgcgccggg caatgtgttc agcgcgtcgc aatcggcggc
     2101 cggcttcctg cgctttaacg tgtcgcaatg caaccggccg aaagtgtacg aggccctgca
     2161 acgggcgatg gatgtgtcgg ccgcagcgag agagcgcgct tgagcgtgct tgagcgcgcg
     2221 ctcgtcgtca cgcgccatga cctgattact tgcatagcaa caacagacgc tcctgatccg
     2281 tgcagggggg acgcggtttc tgcacggctt gcgtcgccgt cgcgctgatc gccgcactct
     2341 tgttcgccag acacgcgcgc aaatgtttct ccaccgggcc gtagtatttc cacccgcgca
     2401 cgagtgtcgg caattccagt ttcacctgct tccatttcgg gtggccgtgt tcctgcagat
     2461 tgtcgaagtt ggcgcacagc gagtcggcaa aatgattcag attgccgacg gtatcgcgca
     2521 gaccgtaatc gtaggtgacg agaaaggcct tcacggttag cgtggggacg tcttccttga
     2581 gccaggtcgg atagctggtc gtacggagcg tggcggggaa atacgtctgt tttgcacgga
     2641 cggtttcggg cgcgttgggg tcggccctta gcgggcggat ctgttgcagc agttcgggat
     2701 tcatgtcgtt gaacagtttg gccggctgcc cgaccacgat gatcgccacg tccaccttct
     2761 tgacgatcaa ggcggccagc gcatcttcgt tactcaggtg ctggatgttc tgctcgggaa
     2821 tcgactggcc aaacatgagg tgatagaggg tcgtggccga ttgagccgtg ccgctgccga
     2881 gcaggccgac gctaatgctc ttgtccttga tgtcggcgaa ggttttcatc ggcgaatcgg
     2941 cgcgcaccac gacgttaatt tcctcgtcgt agagcggcat gatgagccgt agcggccgga
     3001 ttgccgtacc tgcatcggca ttgcccgcgt tggccatgtc gatgtaggcc tgatacacgt
     3061 cggactgcac cagcgcgagc tttacgcccg gctcgaagcg catgcgctgc acgttttcag
     3121 ccgacccctt cgaggccacc acttccaggt cgatgcccac cggctgcgcc acccatttcg
     3181 acaaatcctg gccgatctgg atatacgtgc cccgttccgg accggtgacg atcttgtagt
     3241 gcgcaggctc cgcaccggca aacgaagcca tgaccatcag caacagcccc agccatcccg
     3301 ccagaagtct cttcaacatg gttgatctac ctatcggtca ggattgaaca gcctctcgcg
     3361 gcggcgcgct aagcgcgggt gagcaccggc gcgccggctc gccatctcat gttcaatgcg
     3421 ccgcgcatgc catgtccgtc tgttgccgct cagagggcac acgcgagtgc aatcgggcgt
     3481 gccgtgattc atgcagtcag tcagacaaat cggttgggcc aacggctatg aaattcggta
     3541 tccaaacgat ttgtcgccgg atggattgtc cgtcgcggca tgcggcttaa gtggcgccca
     3601 acctgtttcg cgaattacgc gcgcgaggat cgtcttcgct gtctcgttgc cggtgtcgat
     3661 cgtgagcgcc tcattggcgc gctggcgtgc gcagctccac gactgctgtg caacgcagac
     3721 ttgtgcagcc tgcaacgcgg cgtcgcggcg cgcggtaagc ggcttcaatt ccgtcagcag
     3781 gctttgcgcg tcggcgtcgc cgggttgcag cgattgcgcc gcaccaagcg ctgcttgtgc
     3841 cgtggacagg tcgttggcgc ggaaggccag gcgcgctgtc tgcagcgctt gcgcggcatc
     3901 gcggaactgc ggcgccggtt tgacaggcgc ggcggctacg ggcggtgcga tcggtgaggc
     3961 tgggatcgct ggcgcggcct tgacgggatt ggcgggacta atgggactga tgggactaac
     4021 gggactcaca ggactaacgg ggttaacggc attcatggga gtggtcggca cggcaacggg
     4081 cttacccact gcagcctgat tcgtcgattg agccggggcg tacggcgcaa ttgtcccagt
     4141 cgccgttctc gcatcccgaa gattgtcggc gctctgctcg gtgttgccgc tctgcgattc
     4201 ctggttgtcg ctgaatagtg cataggcgac ataggctagt ccgattgcgc caatggccgc
     4261 gatcgcgatc aacacccggc gcatcgagat cacgccacgg cgggtggaat ccggtggatc
     4321 ggcgagcgga ggaatcggcg catccggcga cgcgagcacc ggcgcttgcg ttggaagctc
     4381 ggcaggggca gcgtcgctcg tgaacgcagt ttgcgcggcg ggcccggttt cgtccggcag
     4441 tgtgggatcg aagctatttt tctgcgccgt tctgttcgtg gcgctcgcac ggctgccggg
     4501 aatcaccgcg cgcttgtgtg gaccggtgtc gagcggatgt accgcgccac aatacgggca
     4561 atagtcgact tgccggtaca gggcaccgcc acagcgtttg catggcgtcg ggaaactatg
     4621 gccgagtagt gtctgggtgg acatacagtg gacccacctg tggaaaacaa tgccgttacg
     4681 acatgttcga gatcatgttg aggcaggagc aatatccgcg gaaatcacga tccacatgca
     4741 ctggagagcc ttgggacgca agtcctcgct tgcggttccg ctggtgtcaa tatgtggcgt
     4801 ttcggcgcga acgtcaatcg acgttctcac ctactttatg tgtgttagcg cacataaagt
     4861 aggtgcaacg agacactgca tatgccggca gaacgaacag accggtggtt tttttcactc
     4921 agcctgggtt gccatggcgc gtatgggaga gcggcacgga gggatcggcg caaccggata
     4981 gcagcgatca aaagagaacg agttcgacgc gcgtggcatt cgacctcgtc agcaagcgct
     5041 tgcagaaaac gcttgtggga attgcttgca gaaagcgcgc ttgcagaaaa ccgctcgtgg
     5101 aaaacgcttg cagaaaccgc tcgtggaaaa cgtgatacga aatcagtgct tgcgtagcgg
     5161 atgatcttta agaagccacg cgagtacgca ggccagcacc accacgcaag cggccgacag
     5221 atacaccgta tgcaacgcac cggcgaatgc ctgcagataa tcgtcgcgca atgcctgagg
     5281 caactggtga attgccgccg gtccgagcgc gtgcggcaaa tcggtgtcgg gtggaatcag
     5341 cttttcgagg cgcccggcga ggccgttgga aaacaccgca ccaaaaccgg ccacgccgat
     5401 cgagccgccg atcgaacgga acagcgtcgc gccggaagtg gccacgccca tgtgcttgaa
     5461 ctcaaccgta ttctgcacgg cgagaatcag cacttgcatc accatgccaa ggccgaaacc
     5521 aagaataccc atgtcgatat aca
//
LOCUS       DATASH010000162         2578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1802659,
            whole genome shotgun sequence.
ACCESSION   DATASH010000162 DATASH010000000
VERSION     DATASH010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1802659"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..105)
                     /locus_tag="VHV99_04755"
     CDS             complement(<1..105)
                     /locus_tag="VHV99_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HEX3634787.1"
                     /translation="MLKVTKAVFPVAGLGTRFLPATKASPKEMLPIVDK"
     gene            complement(178..>2578)
                     /locus_tag="VHV99_04760"
     CDS             complement(178..>2578)
                     /locus_tag="VHV99_04760"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEX3634788.1"
                     /translation="AAVRDVFVRLYEQGLIYRGKRLVNWDPVLLTAVSDLEVVSEEEN
                     GHLWHIQYPLTDGSGHLTVATTRPETMLGDTAAMVHPEDERYAHLIGKTVTLPLSGRE
                     VPIIADDYVDREFGTGVVKVTPAHDFNDYQIGLRHKLPMIEILTLDAKINDNAPEKYR
                     GLDRFEARKQVVADLEALGALESVKPHKLMVPRGDRTGVVIEPMLTDQWFVAMSKPAP
                     EGTFNPGKSIAETALDVVRSGEIRFVPENWSTTYYQWLENIQDWCISRQLWWGHQIPA
                     WYGENGEIFVAKTEEGAREKAAEAGYTGPLKRDEDVLDTWFSSALVPFSSLGWPAETP
                     ELKHFMPSSVLVTGFDIIFFWVARRVMMTTHFTGKVPFDTVYVHGLVRDAEGQKMSKS
                     KGNTLDPIDIVDGIDLDALVAKRTTGLMNPKQAASIEKKTRKEFPDGIPAFGTDALRF
                     TMASMATLGRNINFDLARCEGYRNFCNKLWNATRFVLMNCEGHDCGFGKPEQCGECGP
                     DGHLNFSAADRWIVSRLQRVEAEIAKGFADYRFDNVANALYKFVWDEYCDWYLELAKV
                     QIQTGQPNQQRATRRTLLRVLETVLRLAHPVIPFITEALWQKVAPLAGRYPEGKTEGE
                     ASIMVQPYPVAEPSKIDEEAEQWAADLKAVIDACRNLRGEMNLSPAVKVPLLAAGNAE
                     RLSTFAPYAQALARLSEVQIIADEATLDAQADGAPIAIVGNDKLVLKVEIDVAVERER
                     LSKEIARLSTEVSKCNAKLQNESFVAKAPPAVVEQEQKRLAEFETTIGKLKAQLARLP
                     A"
BASE COUNT          459 a          798 c          768 g          553 t
ORIGIN      
        1 cttgtcgacg atcggcagca tttccttcgg actcgccttc gtggcaggga ggaaccgggt
       61 gccaagacct gctaccggaa ataccgcttt tgtaactttt agcatttgat taccctgaat
      121 cctctggttt agctttgcgc cccgtcagcc cgcaacgggg cggacaaggc gcgtcgatca
      181 ggccggcaaa cgcgccaact gggctttcag cttgccgata gtggtttcga attctgccag
      241 ccttttctgc tcctgctcaa ccactgccgg cggcgccttt gcaacaaagc tttcattttg
      301 caattttgca ttgcacttgc tgacttccgt gctaagccgc gcaatctcct tcgacagacg
      361 ctcgcgctcc accgctacgt cgatttccac cttcagcaca agcttgtcat tcccaacgat
      421 agcaattggc gcgccatctg cctgcgcatc cagcgttgcc tcgtcggcaa tgatctgcac
      481 ctccgacaaa cgggccaatg cctgagcgta cggagcgaag gtggacaggc gctccgcgtt
      541 gccggcagcg agcaacggca ccttgaccgc cggcgacaga ttcatttcgc cacgcagatt
      601 ccggcacgca tcaatcaccg ctttcaggtc cgccgcccat tgttccgcct cttcgtcgat
      661 cttcgacggc tccgcaacgg ggtaaggctg caccatgatg gacgcttcac cctcggtttt
      721 gccttcggga tagcggcccg ccaacggcgc aactttttgc cacagcgctt cggtaatgaa
      781 aggaattacc ggatgcgcga gacgcaacac cgtctcgagt acgcgcagca gcgtgcggcg
      841 tgttgcgcgc tgctggttcg gctgacccgt ctggatctga accttggcga gttcgagata
      901 ccagtcacag tattcgtccc atacgaactt gtatagggcg ttggccacat tgtcaaaacg
      961 atagtcggcg aaacccttgg cgatttccgc ttcgacacgt tgcaggcgcg agacgatcca
     1021 gcggtcggcc gcggagaaat tcaggtggcc atccgggccg cattcgccgc attgctccgg
     1081 cttgccgaaa ccgcaatcgt ggccttcgca attcatcagc acgaaacggg tggcattcca
     1141 cagcttgttg cagaagttgc gatagccttc gcaacgcgcg aggtcgaagt tgatgttgcg
     1201 gccgagcgtg gccatcgacg ccatcgtgaa acgcagcgca tccgtgccga acgccggaat
     1261 gccgtccggg aattccttgc gggttttctt ttcgatcgac gcagcctgct tcggattcat
     1321 cagaccggtg gtgcgcttgg cgaccagcgc gtcgaggtcg atgccgtcga cgatatcgat
     1381 cgggtcgagc gtattgccct tgctcttcga catcttctga ccttcggcgt cgcgcaccag
     1441 accgtgcacg tagaccgtat cgaacggcac cttgccggtg aagtgcgtgg tcatcatgac
     1501 cctgcgcgcg acccagaaga agatgatgtc gaacccggtg accagcactg acgaaggcat
     1561 gaagtgcttc agctccggcg tctcggccgg ccagccgagc gacgagaacg gcactagagc
     1621 agaggagaac cacgtgtcga gcacgtcttc gtcgcgcttg agcgggcccg tgtaaccggc
     1681 ctcggccgcc ttctcgcgcg cgccttcttc cgtcttcgcg acgaagattt caccgttctc
     1741 gccgtaccac gccgggatct gatggcccca ccagagttgg cgcgagatac accagtcctg
     1801 gatgttttcg agccattgat agtacgtggt ggaccagttt tccggcacga atctgatttc
     1861 accgctgcga accacgtcca gcgcggtctc cgcgatcgat ttacccggat tgaaggtgcc
     1921 ctcaggcgcc ggcttgctca tcgcgacgaa ccattggtcc gtgagcatcg gctcgatcac
     1981 cacgcccgtg cggtcgccgc gcggcaccat cagcttgtgc ggtttgaccg attcgagcgc
     2041 gccgagcgct tcgaggtcgg cgacaacctg tttgcgggct tcgaaacggt ccaggccgcg
     2101 gtatttttcc ggtgcgttgt cgttgatctt cgcgtcgagc gtgaggatct cgatcatcgg
     2161 cagcttgtgg cgcaggccga tctgatagtc gttgaaatcg tgcgcgggcg tgactttgac
     2221 cacgccggtg ccgaattcgc ggtcgacgta atcgtcggcg atgatcggga cttcgcggcc
     2281 cgagagcggc agcgtgaccg ttttgccgat caggtgcgca tagcgctcgt cttccggatg
     2341 gaccatcgcg gcggtgtcgc cgagcatggt ttccgggcgc gtggtggcga ccgtcagatg
     2401 gccggagccg tccgtaagcg gatactggat gtgccacagg tggccgtttt cttcttcgct
     2461 gaccacttcg agatcggaga cggcggtgag cagcaccggg tcccagttga cgaggcgctt
     2521 gccgcggtag atcaggcctt gttcatacag gcgtacgaag acgtcgcgca cggcggcc
//
LOCUS       DATASH010000163         5468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5363897,
            whole genome shotgun sequence.
ACCESSION   DATASH010000163 DATASH010000000
VERSION     DATASH010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5468
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5363897"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1230)
                     /locus_tag="VHV99_04765"
     CDS             complement(<1..1230)
                     /locus_tag="VHV99_04765"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX3634789.1"
                     /translation="MNDLTVTRQTFDEVMVPVFSPAAFVPDRGLGSRVWDTQGRDYID
                     FAGGIAVTALGHAHPELLKVLHEQGGKLWHIGNGYTNEPVLRLARRLEDLTFADRAFF
                     ANSGAEANEAALKLARRVAFERHGADKVEIISFTQSFHGRTFFTVSVGGQPKYSEGFG
                     PVPAGIIHLPYNDIEAAKQAIGAKTCAVIVEPIQGEGGVIPADPAFLKALREACDQHG
                     ALLIFDEVQTGVGRSGYFYAYEDTGVTPDILTTAKALGNGFPIGAMLTTNELAAHFKV
                     GVHGTTYGGNPLGAAIAEKVVELISDPKVLEGVRSRSEALKGHLAKLNERFGLFKEVR
                     GKGLLIGAELTDAFKGRAKDFVTAAGQHGVIMLMAGPDVLRFVPSLIMPLDDMNEGFA
                     RLARAIEEVVGAKAETAS"
     gene            complement(1363..2412)
                     /locus_tag="VHV99_04770"
     CDS             complement(1363..2412)
                     /locus_tag="VHV99_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HEX3634790.1"
                     /translation="MARTSNPARTTQVAIVALPPVSMSGVGPIVDALNLANEIDGRAL
                     YRVQVCSWDGRPVPLSGGAQWPADAAFGDAVSCDWLIIVSERFQQFADYRLFLASLSR
                     VGQRTPLVTGIHHGVWWLAMAGQLSGYRVSVNWETYQQFSEQFERSIVTQQIFEIDRD
                     RATCAGGQATVDFMLAMIGRDHGPELADRIADTLGVGVLRAGEERQRIPFVTAPGERH
                     PRLNDALMLMEANIEDPLTTDEIAGLVGVSRRQLERLFRQYLGSMPSKYYLGLRLSKA
                     RTQLQRTSKSVVQISLACGFSSAAHFSNAYRERFGVTPREDRRNWIDKQTGATGAAAS
                     EPRPAALIERPDEAD"
     gene            complement(2450..3241)
                     /locus_tag="VHV99_04775"
     CDS             complement(2450..3241)
                     /locus_tag="VHV99_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3634791.1"
                     /translation="MLHTTQTEACKLAVQDIHKRYGDNEVLKGVSLNANKGDVISIIG
                     ASGSGKSTFLRCINFLERPNAGQIVVDGEMVKTKADRAGNLEVADHKQLQRIRTKLAM
                     VFQHFNLWAHMNVIENIVEAPIHVLGMSRREAEERAREYLEKVGLAPRLEKQYPSHLS
                     GGQQQRVAIARALAMNPDVMLFDEPTSALDPELVGEVLKVMQKLAEEGRTMIVVTHEM
                     GFARNVSNHVMFLHQGRTEEEGLPADVLTTPRSERLKQFLSGSLK"
     gene            complement(3326..4039)
                     /locus_tag="VHV99_04780"
     CDS             complement(3326..4039)
                     /locus_tag="VHV99_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017424506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3634792.1"
                     /translation="MIDILNQFWRAFLYWDGQRMSGLAVTLWLLVASVGIGFCAAIPL
                     AVARVSKKRWLSTPVRLYTYVFRGTPLYVQLLLIYTGMYSLEFVRSHQLLDAFFRSGF
                     HCAILAFALNTCAYTTEIFAGAIRSTSHGEVEAARAYGMSWFTMYRRIVIPSALRRAL
                     PLYSNEVILMLHATTVAFTATVPDILKVARDANSATYRSFDSFGLAALIYLVVSFALV
                     ALFRRAERHWLGYLAVRTH"
     gene            complement(4044..4733)
                     /gene="hisQ"
                     /locus_tag="VHV99_04785"
     CDS             complement(4044..4733)
                     /gene="hisQ"
                     /locus_tag="VHV99_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004527442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HEX3634793.1"
                     /translation="MFLYGFGPVLLAGTIQTIELSVLSLAAAVLLGLAGAAAKLSFNR
                     PLRAIATGYTTLIRSVPDLVLMLLLFYSIQIAVNNLTDALNLPQFDIDPFVAGVLTLG
                     FIYGAYFTETFRGAFLAVPRGQLEAGSAYGMSGARVFTRILFPQMMRFALPGIGNNWQ
                     VLVKATALVSIIGLADVVKAAQDAGKSTFNMFFFILVAALIYLAITTASNLVLIWLEK
                     RYSIGVRHAEL"
     gene            complement(5276..>5468)
                     /locus_tag="VHV99_04790"
     CDS             complement(5276..>5468)
                     /locus_tag="VHV99_04790"
                     /inference="COORDINATES: protein motif:HMM:NF024729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEX3634794.1"
                     /translation="NAVVERFFRSLKTERVRQRAYPDYGAAKTDVTDYILTFYNQQRL
                     HSAAGNMPPAEYETMMQKR"
BASE COUNT         1028 a         1663 c         1706 g         1071 t
ORIGIN      
        1 cgacgcggtt tcggctttcg cgccgacgac ctcttcgatc gccctggcca gacgcgcgaa
       61 gccttcgttc atatcgtcga gcggcatgat cagcgacggc acgaaacgca gcacgtccgg
      121 gcccgccatc agcatgatca cgccatgctg acccgcagcc gtgacgaaat cttttgcgcg
      181 gcccttgaac gcatcggtca attcagcgcc gatcaacagg cccttgccgc gcacttcctt
      241 gaacaagcca aagcgttcgt tcagtttcgc gagatggccc ttcaatgcct cgctgcggct
      301 acgcacgcct tcgagcacct tcgggtcgct gatcagctcg accaccttct ccgcgatcgc
      361 cgcgcccagc ggattgccgc cgtaagtcgt gccatgcacg ccgaccttga agtgcgcggc
      421 cagttcgtta gtggtcagca tcgcgccgat cgggaaaccg ttgccgagcg ccttcgcggt
      481 ggtcaggata tccggcgtca cgccggtgtc ctcgtacgcg tagaagtagc cgctgcggcc
      541 cacacccgtt tgtacctcgt caaaaatcag cagcgcgccg tgctgatcgc aggcttcgcg
      601 caaggccttc aggaatgcag gatcggccgg gatcacgccg ccttcgccct ggatcggctc
      661 gacgatcacc gcgcacgtct tcgcgccgat cgcctgcttt gccgcttcga tgtcgttgta
      721 cggcagatgg atgatgccgg ccggcaccgg accaaagcct tccgaatact tcggctggcc
      781 acccacgctg acggtaaaga acgtgcggcc atggaacgac tgcgtgaacg agatgatttc
      841 gactttgtcc gcgccatgac gctcgaacgc gacgcggcgc gccagcttca gcgcggcttc
      901 gttcgcttcc gcgcccgagt tggcgaagaa cgcgcggtcg gcgaaggtca ggtcttcgag
      961 acgcctcgcg agacgcagca ccggctcgtt ggtgtaaccg ttaccgatgt gccacagctt
     1021 gccaccctgt tcgtgcagta ccttcagcag ttcaggatgc gcatgaccga gcgcggtcac
     1081 cgcgataccg ccggcgaagt caatgtagtc gcggccttgc gtatcccaga cgcgtgagcc
     1141 gagaccgcga tccggcacga aggcggcggg cgaaaacacc ggcaccatga cttcgtcgaa
     1201 ggtctggcgt gtcacagtca ggtcgttcat ggcaaatcct cgttacggtt gagaggtaat
     1261 acacgtagtg taggaaaccg cgggcgatac gtcttgcgca tacgcgacgc tttctatgag
     1321 cttccgggcg gataggtcgc gagtgggtcg cgagtgggtc attcaatccg cctcgtcagg
     1381 ccgttcgatc aacgcggccg gccgcggctc actggccgcg gcgcccgtcg cgccggtctg
     1441 cttgtcgatc cagttacgac gatcctcacg cggtgtgaca ccaaagcgtt cgcggtaagc
     1501 attcgagaag tgcgccgccg acgaaaaccc gcaagcaaga ctgatctgca ccaccgattt
     1561 gctggtgcgc tgcaattgcg tgcgcgcttt cgaaagccgc aggccaagat agtatttgga
     1621 cggcatcgag ccgagatact ggcgaaacag ccgctccaac tgccgccgcg agacgccgac
     1681 cagacccgcg atttcatcgg tggtcagcgg gtcttcgata ttcgcttcca tcagcatcag
     1741 cgcatcgttc agacgcggat ggcgctcgcc cggcgcggtg acgaacggaa tgcgctgccg
     1801 ctcctcgccc gcgcgcaaca cgccgacgcc cagcgtgtca gcgatccggt ccgcgagttc
     1861 aggtccgtga tcgcggccga tcatcgcgag catgaagtcg acggttgcct gaccgcccgc
     1921 gcacgttgcg cgatcacggt cgatttcgaa aatctgttgg gtgacgattg aacgctcgaa
     1981 ctgctcggag aactgctgat aggtttccca gttgacgctc acgcgataac cggacaattg
     2041 ccccgccatc gcaagccacc agacgccgtg atgaataccg gtgacgagcg gcgtgcgttg
     2101 cccgacgcgc gacaaactgg caagaaacag ccggtaatcg gcgaattgct gaaaccgctc
     2161 gctgacgatg atcagccagt cgcacgagac cgcatcgccg aaggcggcat cggccggcca
     2221 ttgcgcgccg cccgacaagg gcaccgggcg gccgtcccac gaacacacct gcacgcggta
     2281 cagcgcccgg ccgtcgattt cgttggcgag attgagcgcg tcgacgatcg gcccgacgcc
     2341 cgacatcgac accggcggca aggcgacgat tgcgacctgc gtggtgcgag ccgggttgga
     2401 cgtgcgagcc atcatcgatt gataaaacgc gaaaagcgcg agccgcgcgt tacttcagac
     2461 tgccggacag gaactgcttg agccgttcgc tgcgcggcgt ggtcagcacg tcggcgggca
     2521 agccctcttc ttcggtgcgg ccctggtgca ggaacatcac gtgattcgac acgttgcgcg
     2581 cgaaacccat ttcgtgcgtg acgacgatca tcgtgcggcc ttcttcggcg agcttctgca
     2641 tcaccttcag cacttcgccg accagttcgg ggtcgagcgc cgaggtgggt tcgtcgaaca
     2701 gcatcacgtc agggttcatc gccagcgcac gcgcaatggc cacgcgctgc tgctggccgc
     2761 ccgacagatg cgacggatac tgcttttcca gacgcggcgc gagacccacc ttctcgagat
     2821 attcacgcgc gcgctcttcg gcttcgcggc gcgacatgcc gagcacatga atcggtgctt
     2881 cgacgatgtt ctcgatcacg ttcatgtgcg cccacaagtt gaagtgctgg aacaccatcg
     2941 cgagtttcgt gcggatgcgt tgcagctgct tgtgatcggc cacttccaga ttgccggcgc
     3001 ggtcggcttt ggttttcacc atttcgccgt cgacgacgat ctgccctgcg tttggccgtt
     3061 caagaaaatt gatgcagcgc agaaaggtgc tcttgcccga cccgctcgcg ccgatgatgc
     3121 tgatcacgtc acccttattc gcgttcagcg acacaccctt gagcacttcg ttgtcgccgt
     3181 agcgcttgtg gatgtcctga acggcgagct tgcaagcttc ggtttgagtg gtgtggagca
     3241 agatgctctc cctttgaaaa tacggatcaa atcagactac gtgacggcgc gcgccagccg
     3301 cgccgctgcg tcgaagcacg ccttgtcagt gcgtgcgcac cgccaggtag cccaaccaat
     3361 gacgctcggc acggcggaac aaggctacca gcgcgaacga caccaccagg taaatcagcg
     3421 ccgcgagtcc gaacgaatcg aacgaccgat acgtggccga attcgcatcg cgcgccacct
     3481 tgaggatatc cggcaccgtg gctgtaaacg cgaccgtggt ggcgtgcagc atcaggatga
     3541 cttcgttact gtacaacggc aacgcccggc gcagcgcgga cggtatcaca atgcggcgat
     3601 acatcgtgaa ccagctcatg ccgtaagcac gggccgcttc cacttcgcca tgcgacgtcg
     3661 agcggatcgc gccggcgaaa atctcggtgg tgtacgcgca ggtgttcagc gcgaaggcga
     3721 gaatcgcgca gtgaaaaccg ctgcggaaaa atgcgtccag caattgatgc gagcgcacga
     3781 attcgaggct gtacataccg gtgtagatca gcaacagttg cacgtacaac ggtgtgccgc
     3841 gaaacacgta agtgtaaaga cgcactggcg tcgacagcca gcgcttcttc gatacgcgcg
     3901 ccaccgcaag cggaatcgcc gcgcaaaagc cgatcccgac cgatgccacc agcagccaca
     3961 gcgtgaccgc caggccggac atgcgctgac cgtcccagta caggaacgca cgccagaatt
     4021 gattgagaat gtcgatcatg gtcttagagc tccgcgtgcc gcacgccgat tgaatagcgc
     4081 ttttccagcc agatcagcac gagattcgac gcggtggtga tcgccagata gatcaaggcg
     4141 gcgaccagaa tgaagaagaa catgttgaag gtgctcttgc cggcgtcctg ggcggctttg
     4201 acgacatcgg cgagcccgat gatcgacacc agcgccgtgg ccttgaccag cacctgccag
     4261 ttgttgccga tgcctggcag cgcgaagcgc atcatctgcg gaaacaggat gcgcgtgaat
     4321 acgcgtgcgc cgctcatgcc gtacgcgctg ccggcttcga gttgaccgcg cggcaccgcg
     4381 agaaacgcgc cgcggaaggt ctcggtgaaa tacgcaccgt agatgaagcc gagcgtcagc
     4441 acaccggcca cgaagggatc gatatcgaac tgcggcaggt tcagggcgtc ggtgagattg
     4501 ttgacggcga tctggatgct gtagaagagc agcaacatca gcacgaggtc gggcaccgag
     4561 cggatcagtg tcgtatagcc ggtggcgatt gcccgcaggg ggcggttgaa ggagagtttc
     4621 gccgctgcgc ccgcgaggcc gagcagcacg gcggctgcca gcgacaggac ggacagctcg
     4681 atcgtctgga tcgtgccggc cagcaacacg ggaccaaagc cgtaaaggaa cacgcgtgtc
     4741 tccatccagg tttattgagg atgtcatgga tggggtcggg gtgggcgggt tcccgactca
     4801 tccggcatgg cgcggagtct cgcaagcgga aattaatttc tcaaaggggc gttctgggtg
     4861 tgctgcggtg cagcaagggg ccgcttgccg cggttggggc ctggtagggc ttggttttgt
     4921 aggggatatc gatgtgtaag gcgaggcgcg cgttgcgagc gttggagcga tttagctggt
     4981 tttgggtcgc tttttcgata ggtggtgggg cgtgcggcag tggcggtttc ttttttgcct
     5041 ccggcagtga ggggttgatt gggtttggtt gcctgctcgg tgcttgttgt ctgtctgtct
     5101 gtctgtctgt ctgtctgtct gtctgtctgt ctgtctgcct gcctgccggc ggcgttggtc
     5161 ttttcttgaa ttggcttttt ggcctttcct tgatttgtta ttggtctatt agcgtcgccc
     5221 ctgtgcgggg cgggcactgt agtggtcaag taaacctgga cacttttccc gcttttcacc
     5281 ttttctgcat catggtttcg tattcggcgg gtggcatatt gccagccgcc gaatgcagcc
     5341 gctgctggtt gtagaacgtc aggatgtaat cggtgacgtc cgtctttgcc gcgccatagt
     5401 ccgggtacgc gcgctgcctc acccgttcgg tcttcaggct ccggaagaag cgctctacta
     5461 ccgcgttg
//
LOCUS       DATASH010000164         2529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_22434,
            whole genome shotgun sequence.
ACCESSION   DATASH010000164 DATASH010000000
VERSION     DATASH010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22434"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="VHV99_04795"
     CDS             <1..702
                     /locus_tag="VHV99_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017335345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX3634795.1"
                     /translation="AGCSIADIAAGMYAYSNILSALLLRGRTGRGCRIDVSMLESMVE
                     WMGYPLYYAMDDQQPPALSGAAHATIYPYGPFPAGDGKTVMLGLQNEREWKLFCEQVL
                     VQPDLATDERFASNSKRTSAREALREVIVAAFAELTAAQVIERLDHAGIANAQMNTLS
                     DVWAHPQLKARERWREIGTSAGAVPALLPPGLPTDFDPRMDPVPALGEQSDAILRELG
                     YDNARIDALRKAGTI"
     gene            804..2192
                     /locus_tag="VHV99_04800"
     CDS             804..2192
                     /locus_tag="VHV99_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX3634796.1"
                     /translation="MTDHPSLTLATFAAQLDFDSIPQAVVERTVNLYVDWLGSALAGK
                     GARPVETIARFARQAGGAVKGGRSEVLIDRGRTTPYFAAMINGAASHFAEQDDVHNGS
                     VFHPATVVFPVALALAQANRVSGRDFIAAAVAGYEVGIRIGEFLGRSHYKVFHTTGTV
                     GTVAAAATAGRLLGLSPTQMLDAFGSAGTQASGLWEFLRDAADSKQLHTAMAAANGLM
                     AAQLASDGFKGATHILEGAQGMAAGMSSDADPARLVDGLGTRWATAETSFKYHAACRH
                     THPAADALLAVVQEHKLAPRDIAKVVAHVHQGAIDVLGAVVAPRTVHQAKFNMGTVLG
                     LVAYHGYAGVTEFEQGFDADSIAAFRDNVEMAFDAEVDAAYPARWIGKVTVTTTDGRT
                     FNGRVDEPKGDPGNTLSRDEIATKLHRLAAFSGAATEGEAAALLGNAWKIAGQPCVEA
                     VFGTEANATVAA"
     gene            2216..2308
                     /locus_tag="VHV99_04805"
                     /pseudo
     CDS             2216..2308
                     /locus_tag="VHV99_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428399.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
BASE COUNT          437 a          781 c          840 g          471 t
ORIGIN      
        1 gcgggttgct cgattgcgga tattgcggcc ggcatgtacg cgtattcgaa cattctcagc
       61 gcgctgttgt tgcgtggccg cacggggcgc ggttgccgca tcgatgtgtc gatgctggag
      121 agcatggtcg aatggatggg ctatccgctg tactacgcga tggatgacca acaaccgccc
      181 gcgctctccg gcgcggcgca cgcaacgatc tatccgtatg ggccgtttcc cgccggcgac
      241 ggcaagacgg tgatgctcgg tctgcaaaac gagcgcgaat ggaagctctt ctgcgagcag
      301 gtgctcgtgc aacccgacct cgccaccgat gagcgctttg cgtcgaattc gaaacgtacc
      361 tcggcgcgtg aggcgttgcg tgaggtgatc gtcgcggcgt ttgccgaatt gactgcggcg
      421 caagtgattg agcggctcga tcatgcaggg atcgccaacg cgcagatgaa tacgttgagc
      481 gatgtgtggg cgcatcccca gttgaaggcg cgtgagcgct ggcgcgaaat cggtacgtcg
      541 gcgggtgcgg tgcccgcgtt attgccgcca ggattgccga cggatttcga tccgcgcatg
      601 gaccccgtgc ccgcgctcgg cgaacagtcc gacgcgatct tgcgtgaact cggttacgac
      661 aacgctcgca tcgacgcgct gcgcaaagcc ggcacgatct gaacggcctt ctttgccttc
      721 gcggccggtg tgcggctcgg gctgcaccgg cggtctgaac aaacctgaca cgcgaccctg
      781 aagctacctt agagcgacta atcatgaccg atcatcccag ccttacgctt gcgaccttcg
      841 cagctcaact cgacttcgac agcatcccgc aggcagtggt cgagcgcacg gttaatttgt
      901 acgtcgactg gctcggctcg gcgctggccg gcaaaggcgc gcggccggtc gaaacgattg
      961 cgcggtttgc gcgccaggcc ggcggcgccg tcaaaggggg ccgaagtgaa gtcctgatcg
     1021 atcgcggccg taccacgccg tatttcgcgg cgatgatcaa tggcgccgcc tcgcattttg
     1081 ccgaacagga cgacgtgcac aacggctcgg tgtttcatcc ggcaaccgtg gtgtttccgg
     1141 tggcgctcgc gttggcgcag gccaatcgtg tgtctgggcg cgacttcatc gccgcggcgg
     1201 tagcggggta tgaagtcggc attcgcattg gcgaatttct cgggcgctcg cactacaagg
     1261 tgtttcatac caccggcacg gtcgggacgg tcgcggccgc tgccactgcg gggcgtttgc
     1321 tcggtttgtc gccgacgcag atgctcgatg cgttcggctc agcgggcacg caagcaagcg
     1381 gcttatggga gttcttacgc gacgccgccg actccaagca actgcacacg gcgatggcag
     1441 cggccaatgg tttgatggcc gcgcaactcg cctccgatgg cttcaagggc gcgacgcata
     1501 ttctcgaagg cgcgcaaggc atggccgctg gcatgtcgag cgacgccgat ccggcgcgcc
     1561 tcgtcgacgg gctcggcacg cgctgggcga ctgccgagac atcgttcaaa taccacgccg
     1621 cgtgccgcca tacacatccc gccgccgatg cgttgcttgc ggtggtacag gagcataagc
     1681 tcgcgccgcg cgatatcgca aaggtggtcg cgcacgttca ccagggcgcc atcgacgtgc
     1741 tcggcgcggt cgtcgcgccg cgcaccgtcc accaggcgaa gttcaacatg ggcacggtgc
     1801 tcgggctcgt cgcctatcat ggctatgcgg gcgtgaccga gttcgaacag ggtttcgacg
     1861 ccgactcgat cgcggccttc cgcgacaacg tcgaaatggc gttcgatgcc gaagtcgatg
     1921 ccgcctatcc cgcgcgctgg atcggcaagg tgacggtcac gacgaccgac ggccgcacgt
     1981 tcaacggacg ggtcgacgaa ccgaaggggg acccgggcaa taccttgtcg cgtgatgaaa
     2041 tcgcgaccaa gctgcatcga ttggcagcgt tttccggggc ggcgaccgaa ggcgaggcgg
     2101 cggccttgct tggcaacgca tggaagatcg ccggacagcc gtgcgtcgaa gcggtgttcg
     2161 gtactgaggc caacgcaacg gtggcagcat gagcgcacga ttgccacgtt cgtagctgtt
     2221 cgtgccgggc aaccggccgg agcgcttcga gaaagcacag gcagcgggcc cggacgcggt
     2281 gattctcgat ctcgaggacg ccgtgcaacg gtaggccctt ccttcaatct gaaatcaagg
     2341 gaaaggcggg cgcacccgag ttcaacccga ttgcgtcccg tactctgtcg tcgcagccgg
     2401 ctttgactta ggcggattcg gaaggctctg ccgcggcgcg tgttgcctgg tcgtgtctgc
     2461 cgttgcaccc accaattgca atgtgctgcc ctgcacctcg atcttcaccg ggtcggtctt
     2521 cttccagag
//
LOCUS       DATASH010000165         1662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6352120,
            whole genome shotgun sequence.
ACCESSION   DATASH010000165 DATASH010000000
VERSION     DATASH010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6352120"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="VHV99_04810"
     CDS             <1..772
                     /locus_tag="VHV99_04810"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HEX3634797.1"
                     /translation="GGVGPAATVDFMAKLVRNTPASRDQDHIKVMVEHNPQIPDRTEA
                     LLGNGDDPTLALYAACKTLEEGGADIIAIPCNTAHAFFERIQPSLSVPIVNMLTCTAD
                     YLRETFPSLREVGVLATSGTLASRVYEKALEVCGFVQIAPAASAQARLMNAIYGPRGA
                     KAGYTSGECCDDLAAAVDGLVAQGVQVIVLGCTELPLLLRGPSLARADGVVVRLVDPT
                     EVLAKRCVAYAMDASGAVNPGGERSACDGAFSARVSAV"
     gene            1197..>1662
                     /locus_tag="VHV99_04815"
     CDS             1197..>1662
                     /locus_tag="VHV99_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634798.1"
                     /translation="MSVTAVQRKAVDPKEYGVPGNVGEGASSAEAVQSKPDGQSARQQ
                     SLRELLRSGPHLTKNDAQGREQNAARPAGTALRSKPDERAGMQASTLGRVQHAMARRG
                     GVPDGRAGDQPGMAAAAPTPFDSTAQANLRDGVGVSASLSGAPMPGKSAALVQ"
BASE COUNT          298 a          472 c          600 g          292 t
ORIGIN      
        1 gggcggtgtt ggcccggcgg ccaccgtcga cttcatggcg aaactcgtgc gcaacacgcc
       61 ggcctcgcgc gatcaggacc acatcaaggt catggtcgag cacaatccgc agattccgga
      121 tcgcactgaa gcactgctcg gtaacggcga cgatccgaca ctcgcgctct acgcggcgtg
      181 caagacgctc gaagagggcg gcgccgatat catcgcgatt ccgtgcaaca cggcgcatgc
      241 gtttttcgag cggattcagc cttcgttgag cgttccgatc gtcaatatgc tgacctgtac
      301 cgcagattat ctgcgcgaga ctttcccgag cctgcgcgaa gtcggcgtgc tggccacctc
      361 gggcacgctt gcgagccggg tctacgagaa ggcgcttgaa gtatgtggct tcgtgcagat
      421 cgcaccggcc gcgagcgctc aggcccggct catgaacgcg atttatgggc cgcgcggcgc
      481 gaaggcgggc tacacgtcgg gcgagtgttg cgacgacctc gccgcggcgg tggatggcct
      541 ggtcgcgcaa ggcgtgcagg tcatcgtgct gggctgcacg gagttgccgc tgctgttgcg
      601 cgggccgtcg ctggcgcggg cggacggagt ggtagtgcgg cttgtcgatc cgaccgaggt
      661 tttggcgaag cgctgtgtgg cgtatgcgat ggacgcgagc ggagcggtga atccgggcgg
      721 tgagcgaagc gcttgcgacg gggcattctc tgcgcgtgtc agtgcggtct gaacggtaga
      781 tgtcaacgcg cgaggcgtcg aaccgatcct catccggcgt gaatcaaaaa aaatgtatag
      841 ctgggatatg tccggttgaa tgcgcgagcc cgttacgatc gaatcgaaga gtgcgtaagg
      901 ccacgcgagt ctgtcgtggc cgatgtgcgg gcgcaggccg tgagtggcag cacatgcgct
      961 gacatcgcga gatgcgcgac ggccgtggat gctcgacggc cgcgcccccg caggtgtctt
     1021 cgtggcgaaa gcggacacgc gtcggcgtac ccaaagccag tcagctgatg cgccatgcct
     1081 ggcggcgttt tgccaggcca ggtagattct gcgcgccgat aactcttcta tggcgattcg
     1141 ccgatgaccg attgatcggt gagtcgggac gtggaccggg aacagggagt atccgaatga
     1201 gtgtaaccgc cgttcagcgc aaggcagtcg atcctaagga gtatggagta ccgggtaacg
     1261 tgggtgaagg cgcgtcgtcc gcagaggccg tgcaatcgaa accggatggg cagtcggcca
     1321 ggcagcagtc gctgcgtgag ctgcttcgtt cggggccgca cctgacgaaa aatgatgcgc
     1381 agggcaggga gcagaatgca gcacggccgg cgggcaccgc tttgcgctcg aagccggatg
     1441 agcgggcggg tatgcaggcg tctacgctgg gtcgagtcca gcatgcaatg gcgcgtcggg
     1501 gtggcgtgcc ggacggtcgc gcaggcgacc agccaggcat ggcagccgca gcacccacgc
     1561 ctttcgatag cacggcgcaa gcgaaccttc gagacggggt gggcgtatct gcttcgttgt
     1621 caggggcgcc catgcccggc aagtcggctg cgctagtgca at
//
LOCUS       DATASH010000166         6135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_615650,
            whole genome shotgun sequence.
ACCESSION   DATASH010000166 DATASH010000000
VERSION     DATASH010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6135
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615650"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="VHV99_04820"
     CDS             <1..236
                     /locus_tag="VHV99_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634799.1"
                     /translation="LAHGLLKSSRILKRCPVVAPPSGAMSSATELSVEKRRLPNNTFQ
                     ISVPARNVRTAAGKGAGLHGTVDALMTPSRVVY"
     gene            264..1673
                     /locus_tag="VHV99_04825"
     CDS             264..1673
                     /locus_tag="VHV99_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3634800.1"
                     /translation="MSSAQQQASRGTNNGLRAFSWLRALRLIFGAPKKYLPYVLPWVL
                     ESLTVAFCYLVVQGVFRQQLLVPPVDTPPSLAERGYTSSFLAEKIMSAMRVIGRDAGS
                     IPHDALTVSDAQPDIQIPGQDLSYATTVRFLKEVVDRKDVVVHIGITKLGDAADSYVA
                     HVQIEGGVFRSRQDEVPFAGRDMDRFVRDIATQAMRLAEPNILASHLFTEVQKTKCSL
                     AQCNYSEIVGIYDEVLALPASKQSEWAEAGKAWLLVSQGLAKEAEAQSREALRRYEGS
                     AILRASLGIALEQQHRIDDALEQLKAGAKESSRTAENLRLLGDVMLHAHRYPEALKAF
                     READRMQPDSVYTLHDWGEALIAVGRYNDAIAKFSQAVSLRPDLAPSYAGWGRALELN
                     GEITEAARRYAQASKLDKDVLSPHEDRIARLSKEYQAVTRSGTPAPDATSHPRLNPPG
                     RRQLEATQSPIGWTAMLTA"
     gene            1725..2207
                     /locus_tag="VHV99_04830"
     CDS             1725..2207
                     /locus_tag="VHV99_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 108 family protein"
                     /protein_id="HEX3634801.1"
                     /translation="MNSFDDAFKALIGSEGGYSFNPADPGGETMWGVTARVARNSGYA
                     GAMKDLPLETAKQIAKEKYWDPLHLDELDPRIAFQIFDANYNGGLVVLWMQKASGAQE
                     DGKFGPDTLAAVKSADPMRFVLRFVAYRLRYLRNLHTWPTFSRGWTDRMATNLLLGAA
                     "
     gene            2207..2902
                     /locus_tag="VHV99_04835"
     CDS             2207..2902
                     /locus_tag="VHV99_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634802.1"
                     /translation="MDWSKVASSIGSTAPLLAGLIGGPIGLGVTAAAAIISHTLGTPN
                     DPQAIETALNDPTALDKLRQAEGANAVQLQQLVVAAAQAHLAYETDMARIQAGDRADA
                     RAMGIANKDWVPKALAMVVTCGFFGILLLMALKELPHENRDLVNVVIGALGTAWISII
                     GYYFGTSAGSMRKTELLAKPSVPITADSAVTLREPAPPPAPRPHAGAGAPHAEMFPAF
                     APGGQGPIFTGGS"
     gene            complement(3152..4156)
                     /locus_tag="VHV99_04840"
     CDS             complement(3152..4156)
                     /locus_tag="VHV99_04840"
                     /inference="COORDINATES: protein motif:HMM:NF005757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX3634803.1"
                     /translation="MQCETLIRRNMLLSSGETLPEAKLVYATAGRLNDARDNVILYPT
                     SFGSSHVDIAWLVGPGRPLDTDRYFVILANMLGNGLSSSPSHYPPGMFPSISVSDSVK
                     LQHTLVHDVLRVERVALVVGFSMGGQQAYHWAALFPEMVERAAIICASARTSVHNQVF
                     LEGLKAALTLDPAWNGRYFSGAAPRGIRAMAQVYAGWGMSAEFYRRALYLTAGYRDLE
                     DFVTNDWGAFLARTNPNDLLAMIDAWHSADISNNARYQGALPNALDAIQARCLVMPSL
                     TDMYFSAAEICDEARQINNVDIHPIDTIWGHRVNNPRQNPADARLVERKLTELLSYTR
                     "
     gene            complement(4158..5435)
                     /locus_tag="VHV99_04845"
     CDS             complement(4158..5435)
                     /locus_tag="VHV99_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3634804.1"
                     /translation="MEISESLGIAEVRPSPHRAIVATVIGNGLEWFDFTVYSYFAVIL
                     AKVFFPTGNDFSSLLLAVATFGVGFFMRPIGGIVLGVYADKKGRKAAMTLTIMIMAAG
                     TTLIAVTPSYQSIGPIAPLIIVVARLMQGFSAGGEMGTAAAFLTEHAPEGRRAYYSSW
                     VLSSIGFAVLLGAAAGTFVTTVLDPASVKSWGWRLPFLLGLLIGPVGFYFRSRITETP
                     VYKKTHRVAASPLRESITTHARDTFGCFAMVMLWTVAIYVLLFYMPTYAASVLHLPPS
                     DGFISGMVGGFAMMCSAPLAGLLADRVGPGRVLAPSAVLVFLLAWPMFTYINAVPGLV
                     SLLVFQAVFGILTGTYTGPILYAFSRRFPSPVLATAISIAYNFGVTVFGGFAAFFVTW
                     LIVATGSTVAPAYYVMLGAAFSLVGMWLLRRGD"
     gene            5586..>6135
                     /locus_tag="VHV99_04850"
     CDS             5586..>6135
                     /locus_tag="VHV99_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008350321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX3634805.1"
                     /translation="MKENGVVSGEPLDYRQVFELCPVACAVLDQRTIVDCNELFASIW
                     RGTRAEIIGLSFAVLYAGESDFDTRGKKIGPILAKRGSYSDNWLMKRLNGEMFWSHVS
                     GTTVDRRAPYARAIWTFLDLSVEPQMVSAIRASLTPREREIAMLLLEGCSSKEIARRL
                     EISPRTVHVHRASLLRKYSVSNT"
BASE COUNT         1300 a         1785 c         1911 g         1139 t
ORIGIN      
        1 gactagcaca tggcctgctc aagtccagcc gaatattgaa acgttgtcct gtcgtcgcgc
       61 ctccatccgg tgcgatgtct tccgcaaccg aactttctgt cgaaaagcgt cggctaccga
      121 acaacacttt ccagatttcc gtgccggcac gaaacgtccg cacagccgcc ggcaaaggcg
      181 cagggttaca tggcacggtc gatgcgttga tgacgccgtc aagagtcgtc tattgaccaa
      241 catcgggccg tgcgaggtgt gtcatgtcca gtgctcagca acaagccagt cgcggaacca
      301 acaacggctt gcgcgccttc tcctggctgc gtgccttaag gttgattttc ggtgcgccca
      361 aaaagtattt gccttacgtg cttccatggg tgctggaaag cctgactgtc gcgttctgct
      421 atctcgtcgt gcagggcgtg ttcaggcaac aactgctggt gccgcccgtg gacacgccgc
      481 cgtctctggc cgaacgcggc tacacatcca gtttcctcgc cgaaaagatc atgtctgcga
      541 tgcgggtgat cgggcgcgac gctggttcca ttccccacga cgcgctgacc gtgagcgacg
      601 cgcagcccga catccagatt cccggtcagg acctgtccta tgcgacgacc gtgcgctttc
      661 tgaaagaggt cgtcgaccgg aaggatgtgg tcgttcatat cggcatcaca aagcttggcg
      721 atgccgcgga ctcctacgtg gcgcacgtgc agatcgaagg tggtgtgttc cggtcgcgcc
      781 aggacgaggt gccgttcgcg ggacgcgata tggacaggtt cgtgcgcgat atcgctacgc
      841 aggcgatgcg gcttgccgag ccgaacatac tggccagcca tctgttcacc gaagtgcaga
      901 aaacgaaatg ctcgcttgcg caatgcaact acagtgagat cgtcggcatc tacgatgaag
      961 tgcttgcgct gcccgcttcg aagcagagcg aatgggcgga ggcaggcaag gcgtggttgc
     1021 tggtgagcca gggcctcgcg aaagaggcgg aagcgcagag ccgcgaggcg ctgcgccgct
     1081 atgagggctc ggcgattctg cgggctagcc tcgggattgc gctcgagcag cagcaccgaa
     1141 tcgacgacgc gctggagcaa ctcaaggcgg gtgcaaaaga atcatcgagg accgccgaga
     1201 acctgcgtct gctgggcgac gtgatgttgc atgcacatcg ttatccggaa gcgctgaagg
     1261 ctttcaggga agccgatcgt atgcagccgg actcggtcta cacgctgcac gactggggtg
     1321 aggcgcttat cgcggtcgga cgctacaacg atgcgattgc aaagttctct caggccgtat
     1381 cgcttcgccc ggatctcgcg ccgtcgtatg cggggtgggg ccgtgcgctc gagctaaatg
     1441 gcgagattac cgaagcggcg cgccggtatg cgcaggcatc gaaactcgac aaggatgtgc
     1501 tctcgccgca cgaagaccgt atcgcgcggc ttagcaagga gtatcaggcg gtcacgcgtt
     1561 ccggtacgcc ggctccggat gcgacgagcc acccacgttt gaatccgccc ggaagacgtc
     1621 agcttgaagc aacacaaagc ccgatcggat ggaccgcgat gctgacggct tagcgctaag
     1681 gcatcttgtt tagcagtatc acgtagcagg aaaggagaca gtgaatgaac agcttcgacg
     1741 atgcgttcaa ggctctgatc ggaagcgaag gaggatattc cttcaatccc gcggaccccg
     1801 gcggcgaaac gatgtggggc gtgacggcac gcgtggcgcg taactccggt tatgccggcg
     1861 cgatgaagga cctgccgctc gaaaccgcaa agcagatcgc caaggaaaaa tactgggacc
     1921 cgctgcatct ggatgaactc gatccgcgga tcgccttcca gatattcgat gcgaactata
     1981 acggcggtct cgtggtgttg tggatgcaga aggcgtccgg cgctcaggaa gacggcaagt
     2041 tcggcccgga cacgctggcc gccgtcaaga gcgcggaccc gatgagattt gtcctgcgat
     2101 tcgtggcgta tcggctccgg tatctgcgaa acctgcatac gtggccgacc ttcagccgcg
     2161 gatggacgga ccgtatggca accaaccttc tcctgggagc agcatgatgg actggtcaaa
     2221 agtagcgtcg agcatcggca gcacggcccc tttgcttgcc ggcctgatag gcggcccgat
     2281 cgggttgggt gtgacggccg ccgccgccat catttcgcat acgctgggca cgcccaacga
     2341 tccgcaggcg atcgagaccg cgctgaacga cccgacggca ctcgacaagc tcagacaggc
     2401 cgagggcgcc aacgcggtgc agttgcagca gctggtggtg gccgcggcgc aggcccatct
     2461 cgcgtacgag accgacatgg cacgcatcca ggcaggcgat cgggccgatg cgcgcgcgat
     2521 ggggattgct aacaaggatt gggtgcccaa ggccctcgcg atggtggtga cgtgcggctt
     2581 cttcggcatc ctgctgctga tggcgttgaa ggaattgcct cacgaaaacc gcgatctggt
     2641 caacgtggtg atcggcgcgc tcggcacggc gtggatcagc atcatcggct attactttgg
     2701 tacgtcggcg ggttcgatga gaaagacgga actgctcgcc aagccgagtg tgccgatcac
     2761 cgcggatagt gcggtcacgc tgcgggaacc ggcgccgccg ccggcacccc ggccgcatgc
     2821 tggagcgggc gcgccgcacg ccgagatgtt cccggcgttt gcgccgggcg ggcagggccc
     2881 gatcttcacc ggcggatcct gatccagggt tggaacggcc ggtgcgcgcg acgaggcgtt
     2941 gtgctgttgt gctgtcgcgc gcagtgagat gaacttcggc attcacgctc tgttggcgat
     3001 tgccggtttc gcgttcgtcg tcggcacgat caggcgcatc gatcccgtct agcgtgcgcg
     3061 tcggctgctc gcgagactgt gcgaagccag ggttgctttg caactccgcc cacattgacg
     3121 tgacgacaca cacagacggc aacccaacga atcaacgcgt atagctaagt agctcggtga
     3181 gcttgcgctc caccaatcgg gcgtcagcgg gattctgtct ggggttattg actcgatggc
     3241 cccagatcgt atcaatggga tggatgtcga cgttattgat ctgccgggct tcgtcgcaga
     3301 tctctgcggc ggagaagtac atatccgtca ggcttggcat gaccaggcat cgcgcctgaa
     3361 tggcatcgag cgcattcggt aaagcacctt ggtagcgcgc gttgttgctg atgtccgcgc
     3421 tgtgccaggc gtcgatcatg gcaagcagat cgtttgggtt cgtacgggcg agaaacgcgc
     3481 cccagtcgtt agtcacgaaa tcttcgagat cgcggtatcc ggccgtgaga tataacgcgc
     3541 gtctgtagaa ttccgcggac atgccccacc ctgcatagac ttgcgccatc gcgcgaatgc
     3601 ctcgcggcgc cgcgccggag aaataccggc cgttccaggc cggatcgagc gtgagcgctg
     3661 ccttcaatcc ttccagaaat acctggttat gaaccgacgt tcttgcagac gcgcaaatga
     3721 tcgcggcgcg ctccaccatt tcaggaaata gggcggccca gtgataggcc tgctgtcccc
     3781 ccatcgagaa cccaacgacc aaagctacac gctccacgcg caacacatcg tgaacgagtg
     3841 tgtgctggag cttcacgctg tcgctgacgg atatcgacgg aaacatgccc ggcgggtagt
     3901 gactcggtga tgacgacaag ccgttaccaa gcatattcgc caggatcacg aaatagcggt
     3961 cagtgtcgag cggccggccc gggccgacca gccatgctat gtcaacatgg ctgctgccga
     4021 acgacgtggg atagagaatt acgttgtcac gtgcgtcatt gagacggccg gccgtcgcgt
     4081 agacgagctt cgcttcgggc agtgtttcgc cactgctcaa tagcatattc ctgcggatta
     4141 aggtctcgca ttgcatatca atcaccccgc cgcagcagcc acatgcctac cagactgaaa
     4201 gccgcgccga gcatgacgta gtacgctggc gcgacggtgc tgccggtggc gacgatcagc
     4261 catgtcacga aaaacgccgc gaaaccgccg aacaccgtaa cgccaaagtt gtacgcaatc
     4321 gatatggccg tcgcaaggac ggggctcgga aaacgtcgtg aaaacgcgta caggatcgga
     4381 cccgtatagg tgccggtcag aatgccgaag acagcctgga acaccagcag cgacaccagg
     4441 cccggcacag cattaatgta ggtaaacatc ggccacgcga gcagaaaaac aagtaccgcc
     4501 gatggcgcca acacccgtcc cggtcccacc ctatcggcga gcaaaccggc aagtggcgcg
     4561 ctgcacatca tggcaaagcc tccgaccatt cccgaaatga aaccgtccga cggcgggaga
     4621 tgcaggacac ttgccgcata cgtcggcata tagaacagca gcacgtagat ggcgactgtc
     4681 catagcatga ccatcgcgaa acagccgaac gtgtctctgg cgtgcgtcgt gatggattcc
     4741 cgcagcggcg acgcagcaac acgatgtgtt ttcttataga cgggtgtttc agtaatcctg
     4801 gagcggaaat aaaaccccac cggaccgatc aggaggccga gcaggaacgg caaacgccac
     4861 ccccaacttt tgacggacgc aggatcgagg accgttgtca cgaacgtgcc ggcggcggcg
     4921 ccaagcagga cggcgaagcc aatgctcgac agcacccagc tcgagtaata ggcccggcgg
     4981 ccttcaggcg catgctcggt cagaaaagcc gcggccgtgc ccatttcacc gcccgcggaa
     5041 aagccctgca tcagacgcgc gaccacgatg atcaatggcg cgatcgggcc gatcgattgg
     5101 tatgacggcg tcaccgcgat caaggtcgtg ccggcggcca tgatcatgat ggtgagcgtc
     5161 atggcggcct tacgcccttt cttgtccgca tagacgccca gcacgatgcc gccgatagga
     5221 cgcatgaaaa acccgacgcc gaatgtcgca accgcaagca gcagcgaact gaaatcgttt
     5281 ccggtcggga aaaacacttt ggccagaatc acggcgaaat agctgtacac cgtgaaatcg
     5341 aaccactcga ggccgttgcc gataacagtc gccacgatag cccgatgtgg gctcggccgg
     5401 acttctgcga tgcccagcga ttccgatatt tccatttttg ctccgtgaag tgtcggccag
     5461 ggttgcgcgt gatcgacctt ttacttattc gactaggtaa tgcagaatcg agtttaacgg
     5521 gccgaaaacg tgattcaaaa tgtttcgttt acctaggtaa gcgctgcgaa gggagagggt
     5581 tgattgtgaa agagaacggg gttgtgtctg gcgaaccact tgattaccgt caggttttcg
     5641 agctctgccc tgtcgcctgc gctgttctgg atcagcgcac catcgtcgat tgcaacgagc
     5701 tgttcgcgag catctggcga ggaacgcgcg ccgagataat cgggctgtcg ttcgcggtgc
     5761 tttacgctgg cgagagcgac ttcgatacgc ggggaaagaa gattggtcca atactcgcga
     5821 agcgcggcag ctactccgac aactggctga tgaagcggct gaatggagag atgttctgga
     5881 gccatgtaag cgggacgacc gtcgacaggc gcgccccgta tgcccgcgca atctggacat
     5941 ttttagattt gtcggttgaa ccgcaaatgg tgtcggccat acgtgcttcg ctcacgccgc
     6001 gcgagcggga aatcgccatg ctgctgctcg agggttgcag cagcaaggaa atcgcgcggc
     6061 ggctggagat cagtccacga actgtgcatg ttcatcgtgc aagcttgctg cgcaaatact
     6121 cggtcagcaa cacgg
//
LOCUS       DATASH010000167         5341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4968375,
            whole genome shotgun sequence.
ACCESSION   DATASH010000167 DATASH010000000
VERSION     DATASH010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4968375"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..165)
                     /locus_tag="VHV99_04855"
     CDS             complement(<1..165)
                     /locus_tag="VHV99_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006052234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEX3634806.1"
                     /translation="MDLTQVSSSSSSASRPDKAFASDSLNVAQFDERPFLKRLGIADW
                     LFAVVMVAGAG"
     gene            complement(170..2392)
                     /locus_tag="VHV99_04860"
     CDS             complement(170..2392)
                     /locus_tag="VHV99_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HEX3634807.1"
                     /translation="MSVTTSGLQSKSGNRITRSIIEVLSSMRFAIALLVILSIASIIG
                     TVLTQDDPYPNYVNQFGPFWADIFRSLSLYTVYSSWWFMLILGFLMVSVSLCVIRNAP
                     KMVADAKSWKDKVREGSLRAFHHKGEFAVHGTRAQTSAVLAKLSGKLGYKFVTRESEG
                     ATLIAAKRGALTKFGYISAHIAIVVICLGGLLDSNLPIKLQMWLFDKSPIRANTVIND
                     IAPEHRLSQSNPSFRGYAWVPEGQHVSTAILNQPDGSLIQDLPFSIELNKFIVDYYST
                     GMPKLFASDIVVVDHKTGARVPARVEVNKPFTYDGVSIYQSSFQDGGSQMQMTAYPMA
                     GNSAKTAPFGGTIGGNAPLKAATPLADGQTVEFTDFRAINVENVSNGSGQNDARGVAA
                     HRTLKEAFDERLGSGAKTSNPLDLHNVGPSVQYKVRDKDGQAREYNNYMLPVDVAGEK
                     MFLAGMRVNPDDPFRYLRIPADTGGTVKEWMNLRATLEDPAMRATAAHRFALRSVPGS
                     NAELQQHLEESALRVLTLFAGGDNSIKMPNGQAVGGFQAIAAFIDHSVPKGEQEKAAS
                     LLLRMLEGATWDLWQLSRQQLGEPDAQANTDASRFVQSSLNAISDSFLYGSPVYLQLD
                     SFKQVQASVFQLTRAPGKKVVYLGSLLLVLGIFSMFYVRERRLWFWLKDTDHGTNVVM
                     AMSSARKTLDFEKEFIQTRDAVGAALGTKLTEASDAAGVVNKPGNAGTPSASSQDSTR
                     "
     gene            complement(2510..3163)
                     /locus_tag="VHV99_04865"
     CDS             complement(2510..3163)
                     /locus_tag="VHV99_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX3634808.1"
                     /translation="MNRLCKALMAFQLAAGLSGLAIQARAADPAKPDVNRGQAIAVQV
                     CASCHGADGNSAGGAYPKLAGQHPEYLVKQLMDFKTQPGAKQPARNNAIMAGMAAALS
                     DQDMVNVAAYFSTQTPKPGYAHNKDTVPLGQKIYRGGIADKGVPACASCHGPTGQGIP
                     SQYPRLSGQWAEYTVAQLMAFTQGPGARNNNEPMHAIATRLSDSDIKTVADYIAGLH"
     gene            3390..4043
                     /gene="yihA"
                     /locus_tag="VHV99_04870"
     CDS             3390..4043
                     /gene="yihA"
                     /locus_tag="VHV99_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEX3634809.1"
                     /translation="MSFLLHQSRFFTTVNHLRDLPATAQPEICFAGRSNAGKSTAINI
                     LCNQKRLAFASKTPGRTQHINYFSVGKADEPTAHLVDLPGYGYAEVPGAAKAHWEALL
                     STYLQSRSQLRGMILMMDSRRPLTDLDRRMIEWFAPTGKPIHTLLTKCDKLTRQESIN
                     ALRATQKGLAEYRTAGYQGELTAQLFSALKRVGIDEAHELIESWLIPDAKGEADAPQ"
     gene            4305..5303
                     /gene="hemB"
                     /locus_tag="VHV99_04875"
     CDS             4305..5303
                     /gene="hemB"
                     /locus_tag="VHV99_04875"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009913842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX3634810.1"
                     /translation="MSFYPHYRPRRMRRDDFSRRLMRENILTTNDLIYPVFIVEGTNV
                     RQAVPSMPGVERVSVDLLMGVAEQCVELGVPVLSLFPAIEPSLKTPDGREATNPAGLI
                     PRAVRELKKNFPDLGVLTDVALDPYTSHGQDGVLDEAGYVINDETVEILVEQARAQAE
                     AGVDIVAPSDMMDGRIGAIREMLESEGYIHTRIMAYAAKFASAFYGPFRDAVGSATNL
                     GKGNKMTYQMDPANSDEALREVQADIEEGADMVMVKPGMPYLDIVRRVKDEFQFPTYV
                     YQVSGEYAMLKAAAQNGWLDHDKVMMESLLAFKRAGADGVLTYFALDAARLLRAQR"
BASE COUNT         1053 a         1631 c         1597 g         1060 t
ORIGIN      
        1 ccccgcgccc gcaaccatca cgacggcgaa cagccagtcg gcgataccga gccgtttcag
       61 aaacggccgc tcgtcgaatt gcgcgacgtt gagcgagtcg ctcgcgaacg ccttgtcggg
      121 ccgcgaagct gaagaagagg atgaggaaac ctgagtcaag tccatgatct taccgggtcg
      181 aatcttgtga gcttgcggac ggcgtgccgg cattgccggg cttgttgacg acgccggcgg
      241 cgtcggatgc ttcagtgagc ttggtgccca acgcggcgcc tacagcgtcg cgcgtttgga
      301 tgaactcctt ttcgaagtcg agcgttttgc gcgcgctcga catcgccatc acgacattcg
      361 tgccgtgatc ggtatctttg agccagaacc agaggcgccg ttcgcggacg tagaacatcg
      421 aaaagatgcc caacacgagg agcaggctgc caagatacac gacttttttg cccggcgcgc
      481 gcgtcaactg aaataccgaa gcttgcacct gcttgaagga gtcaagctgc aagtagaccg
      541 gcgatccata caaaaagctg tcagatatcg cattgagcga actctggacg aagcggctgg
      601 cgtccgtatt ggcctgtgca tcgggctcgc ccaactgctg acgcgacaat tgccacagat
      661 cccacgtcgc gccttccagc atgcgcaaca gcagactcgc agctttttcc tgctcaccct
      721 tcggcacaga gtggtcgatg aaagccgcga ttgcctggaa gccgccgacc gcctggccgt
      781 tcggcatctt tatgctgttg tctccgccgg caaacagggt caggacacgc agcgcgcttt
      841 cttccaggtg ctgttgcaat tcggcattcg aacccggcac cgaacgcaga gcgaaacggt
      901 gcgccgccgt ggcgcgcatg gccggatctt caagtgtggc gcgcaggttc atccattcct
      961 tgacggtacc gccggtatcg gccggaatgc gcagatagcg gaatgggtcg tccggattca
     1021 cgcgcatgcc ggcgaggaac attttttcgc cggcgacgtc caccggcagc atgtagttgt
     1081 tgtactcgcg cgcctggccg tccttgtcgc gcaccttgta ttgcaccgac ggaccgacgt
     1141 tatgcagatc gagtggattc gaggtcttgg cgccggagcc gagacgctcg tcgaacgcct
     1201 ctttcagcgt gcgatgcgcg gcgacaccgc gagcatcgtt ctggccgctg ccgttcgaca
     1261 cgttttccac gttgatcgcg cggaaatcgg tgaattcgac ggtttggccg tcagccagcg
     1321 gcgtggccgc tttcaacggt gcgttgccgc cgatcgtgcc gccgaacggc gcggtcttcg
     1381 cgctgttgcc ggccatcgga tacgcggtca tctgcatctg cgagccgccg tcctgaaaac
     1441 tcgactgata gatcgacacg ccgtcatacg tgaacggctt gttcacttcg acgcgcgccg
     1501 gcacgcgtgc gccggtcttg tgatcgacta ccacgatatc gctcgcgaac agctttggca
     1561 tgccggtcga gtaatagtcg acgatgaatt tgttgagttc gatcgaaaac ggcaggtcct
     1621 ggatcagcga gccgtccggc tgattcagaa tcgccgtcga cacatgctgt ccttccggca
     1681 cccatgcgta accgcggaaa ctcgggttcg attgcgacag gcgatgttcc ggcgcaatgt
     1741 cgttgatcac cgtgttggcg cggatcggcg acttgtcgaa cagccacatt tgcagcttga
     1801 tcggcagatt gctgtcgagc agaccgccca gacagatcac tacgatcgca atgtgcgcgg
     1861 agatgtagcc gaatttggtc aacgcgccgc gcttggccgc gatcagcgtt gcgccttccg
     1921 actcacgtgt gacgaacttg tagccgagct tgcccgagag tttggcaagc accgccgagg
     1981 tctgcgcgcg cgtgccgtgc accgcgaact cgcccttgtg atggaacgcg cgcaggctgc
     2041 cttcgcgcac cttgtccttc cagctcttgg cgtccgccac catcttcgga gcattgcgga
     2101 tcacgcacag cgacaccgac accatcagga aaccgagaat cagcatgaac caccacgagc
     2161 tgtacaccgt gtacagactc agcgagcgga agatgtccgc ccagaacggg ccgaactgat
     2221 tgacgtagtt gggataggga tcgtcctgcg tgaggacggt accgatgatg ctggcgatcg
     2281 acagaatcac gagcagcgcg atcgcgaagc gcatcgaact caatacttcg atgatgctgc
     2341 gcgtgatgcg attgcccgac ttcgactgca aacccgacgt ggtgacgctc attcatactc
     2401 cgactgaaca gcaaaaaagg gtgaagggcc gtcgtaggca cgacgccctc acccttttct
     2461 tgttttgtac cgacctcgcc gggccggttg cactagcgtg cgggctacct taatgcaagc
     2521 ccgcgatgta atcggctaca gtcttgatgt cactatccga cagacgtgtt gcaattgcat
     2581 gcatcggctc gttgttgtta cgtgcgcccg gaccttgcgt gaatgccatc aactgcgcca
     2641 ccgtatattc ggcccactgc cccgacagac gtggatactg cgacggaatc ccttgaccgg
     2701 tcggcccgtg acagcttgcg catgccggta cgcccttgtc cgctatgccg ccgcgataaa
     2761 tcttctgacc gagcgggacg gtgtctttgt tatgcgcgta gcctggcttc ggagtctgtg
     2821 tcgaaaaata ggcggccacg ttaaccatgt cctgatcgga caacgccgcg gccatgccgg
     2881 ccatgatcgc attgttgcgc gcgggttgct tggcgcccgg ctgcgtcttg aaatccataa
     2941 gctgtttgac gagatactcg ggatgctggc ccgccagctt cggatacgca ccgccggcgc
     3001 tgttgccgtc cgcaccgtga catgacgcgc aaacctgcac cgcgattgcc tgaccccgat
     3061 tgacgtccgg ctttgccgga tctgctgctc gtgcctgaat tgccaaacct gaaagacctg
     3121 ccgctagttg aaacgccatc agagccttgc acagtcgatt cattcgcaca ccctgtctcg
     3181 tcttgtggga ttttagaggt tttgcaagat acgacagcga ctgagtgacc ctgagtgagc
     3241 actaagagcc gccaaggcag gtggggcggc cccctcaggt tttcagtaaa ccgtcgtatt
     3301 gtacaataac tcgctaatcg ctaagacgcc agtcgggctt gacccgtccc caatccgggt
     3361 tccccggcgt ctcgaatcct ggcctgatca tgtccttcct gctccaccaa tcccgctttt
     3421 tcacgaccgt caaccacctg cgtgacctgc cggccacggc gcaacccgag atctgctttg
     3481 ccgggcgctc gaatgcgggc aagtcaaccg ccatcaatat tctgtgcaat cagaagcggc
     3541 tggccttcgc cagtaagact ccggggcgta cgcagcacat caactatttc tcggttggaa
     3601 aagcggacga gccgactgca catctggtcg acctgccggg ttacggctat gcggaagtgc
     3661 cgggcgcggc caaggcccac tgggaagcac tgctgtccac ttatctgcaa tcgcgctcgc
     3721 agttgcgcgg catgatcctg atgatggact cgcggcgccc gttgacggat ctggatcggc
     3781 ggatgatcga gtggttcgcg ccgaccggca agccgattca cacgctgctg acgaaatgcg
     3841 acaaattgac acgccaggaa agcatcaacg ccttgcgcgc gacgcagaaa ggtttggctg
     3901 aatatcgtac cgccggctat cagggcgaat tgaccgcgca gttgttctcg gcgctcaagc
     3961 gcgtcggcat agacgaagcg catgagttaa tcgaaagctg gctgatcccc gacgcgaagg
     4021 gtgaagcaga cgccccgcag taaccgcaca gcacgtatga gcgcaggcaa aaagcgcctt
     4081 tttggcccat ttcggccact cataaaaaaa cccgccgctt taacggcggg tcaaacagcc
     4141 taatcgaaaa aacgacaggc acccgctcag ggaggagaag cggggaggtc agcgccaggc
     4201 gccttgctcg atcggtatga tataccattg tctcgaaaag tttccgggag cggaggcagg
     4261 cgttacgtgt ccactccaca catccacgtt tcttcgatgc gtctatgagc ttctacccgc
     4321 actatcgtcc gcgccgcatg cgccgtgacg acttctcgcg ccgtctgatg cgagaaaaca
     4381 tcctcaccac caacgacctg atttaccccg tgttcatcgt cgaaggcacc aacgtgcggc
     4441 aagccgtacc gtcgatgccg ggcgtcgagc gtgtgtcggt cgatctgctg atgggtgtgg
     4501 ccgaacaatg cgtcgagttg ggcgtgcccg tgctgtcgct gttcccggcg atcgagcctt
     4561 ccttgaagac gcctgatggc cgcgaagcga ctaatcccgc cggtttgatc ccgcgtgccg
     4621 ttcgcgagct gaaaaagaac ttccccgacc tgggtgtgct taccgatgtc gcgctcgatc
     4681 cgtacacgag ccacggccag gacggcgtac tcgacgaggc cggctacgtg atcaacgacg
     4741 agaccgtcga gattctcgtt gaacaggcgc gtgcacaggc tgaagcgggg gtcgatatcg
     4801 tcgcgccctc ggacatgatg gacggccgca tcggcgcgat ccgcgaaatg ctcgagagcg
     4861 agggctatat ccacacacgc atcatggctt acgcggccaa gttcgcgtcg gcgttttacg
     4921 gcccgttccg cgatgccgtc ggttccgcga cgaacctcgg caagggcaac aagatgacct
     4981 accagatgga cccagccaac tcggatgaag cgctgcgcga agtgcaagcg gacatcgaag
     5041 aaggcgcgga tatggtgatg gtcaagccgg gcatgccgta tctggacatc gtgcgtcgcg
     5101 tgaaggacga gttccagttc ccgacctacg tgtatcaggt cagcggcgaa tacgcgatgc
     5161 tgaaggcggc cgcgcagaac ggctggctcg atcacgacaa ggtgatgatg gagtcgctgc
     5221 tggcgttcaa gcgcgcgggc gccgacggcg tgctgacgta cttcgcgctc gacgccgcgc
     5281 ggctgctgcg cgcgcagcgc taggcgccgc tgctgccggc gttggcgttg ccctcggcgt
     5341 t
//
LOCUS       DATASH010000168         3430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2594709,
            whole genome shotgun sequence.
ACCESSION   DATASH010000168 DATASH010000000
VERSION     DATASH010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2594709"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1347
                     /locus_tag="VHV99_04880"
     CDS             <1..1347
                     /locus_tag="VHV99_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase,
                     adenosylcobalamin-dependent"
                     /protein_id="HEX3634811.1"
                     /translation="VRIARLMRDEAYRASVELARERGAFELFDAGRYLEAGTFASRLP
                     DDIQEAIRRDGIRNSHLLSIAPTGTVSLAFADNASNGIEPAFSWTYTRMKVMADGGRE
                     SFDVEDYAYRLYRELGGDVKNLPDYFVSALEMSARDHLDMMAAVQPYVDTSISKTVNV
                     PADYPFAAFESLYFDAWKNGLKGLATYRPNETLGAVLSVSPPQADDTLAETDQDPLRI
                     AIDHRPKGELPAIIEKVEYLTQAGKKSLYVAVSFIEVTGRLGGEDVTIERPIEFFIPT
                     GQRDESQQWITATMRSLSLAARGGFVARNLQDMRKVSWDRGQVRLGEVQRLDGHRTPR
                     WHDSEVAALAFAIQQILHRRGFLDAEGNQVPSRMLARLPRGQMRAETALPADFGIRPP
                     PGGTGAADDAALLQPGGAQGLHTMLGRKCGSCGANAVIRKDGCDFCTACGEVGACG"
     gene            complement(1508..2203)
                     /locus_tag="VHV99_04885"
     CDS             complement(1508..2203)
                     /locus_tag="VHV99_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX3634812.1"
                     /translation="MPASKVAGPKAPYTNEEIYGRIQKAVLEHRLAPGTKLIEERLAE
                     VSGVSRTKIREVLNKLAHEGLVELVPNRGAFIASPSVQQARDVFVTRRMIEPEMARLL
                     SRTATSAQVRALRKHVRQESDARKQNDRPAIIRLSGEFHVLIAEMVGNEILLRMMREL
                     APLTCLVITLYDKPNAPACPHHEHGDLIDAIEAHDGAKAVACMLEHLEHIEATLNFDE
                     VKGASPDIEAIFS"
     gene            2868..>3430
                     /locus_tag="VHV99_04890"
     CDS             2868..>3430
                     /locus_tag="VHV99_04890"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX3634813.1"
                     /translation="MSERFSRRQFLQRAGAGAALVSIGLPRFAMAAGLTLDGIKKAGV
                     LRIGCEATYPPLTYRDASGTMIGYDVDLANAFCRNLGVKAQFVDTVWSGVIPSLYTKK
                     FDLVMSSLSYTVEREKHVDFSVPYVEASQALLIRAADQKSVTGIAGMNGKVLGLKLGS
                     PGQILEPKLDAQLKAAGGAGFKDVKTF"
BASE COUNT          643 a         1027 c         1055 g          705 t
ORIGIN      
        1 gtgcgcatcg cccggctgat gcgcgacgaa gcctatcggg cgtcggtgga actggcgcgc
       61 gagcgcggcg cgttcgaatt gttcgacgcc gggcgttatc tggaagcggg caccttcgcc
      121 tcgcgcctgc ccgacgacat ccaggaggcc atccgccggg acggcatccg caacagccat
      181 ttgctgtcga tcgcgccgac cggcaccgtc agcctcgcgt tcgccgacaa cgcctcgaac
      241 ggcatcgagc cggccttctc gtggacctac acgcgcatga aggtgatggc cgacggcggc
      301 cgcgagtcgt tcgatgtcga agactacgcg taccgtcttt atcgcgagct cggcggcgat
      361 gtgaaaaacc tgcctgacta cttcgtcagc gcactcgaaa tgtcggcacg cgaccacctc
      421 gacatgatgg ctgccgtgca gccatacgtg gacacgtcga tctcgaagac ggtgaacgtg
      481 ccggcggact atccgttcgc ggcattcgaa agcctctact tcgacgcgtg gaaaaacggg
      541 ctcaagggtc tcgccaccta ccgtccgaac gaaacgctcg gcgcggtgct gagcgtgagc
      601 ccgccgcaag cggatgacac gctggccgag accgatcagg atccgctgcg gatcgcgatc
      661 gatcatcggc ccaagggcga attgccggcg atcatcgaaa aggtcgagta tctgacgcaa
      721 gccggcaaga agtcgcttta cgtggcggtg tcgtttatcg aagtgacggg acggctcggc
      781 ggtgaagacg tcacgatcga acgccccatc gagtttttca ttcccaccgg tcagcgcgac
      841 gagtcgcagc aatggatcac ggcgacgatg cgttcgctgt cgctcgccgc acgcggcggt
      901 tttgtcgcgc gcaacttgca ggacatgcgt aaggtgtcgt gggaccgtgg gcaggtgcgg
      961 ctcggcgagg tgcagcgtct tgacggtcat cgcacgccgc gctggcacga ttccgaagtg
     1021 gcggcgctcg cgtttgcgat ccagcagatt ctgcatcggc gcggctttct cgatgccgaa
     1081 ggcaaccagg tgccgtcgcg catgctggcc cgtttgccgc gcgggcagat gagggccgag
     1141 acggcattgc cggcggattt cggcatccgg ccgccgcccg gcggcaccgg cgcggctgac
     1201 gatgccgccc tgctgcagcc cggcggtgca caaggactgc acaccatgct tggacgcaag
     1261 tgcggctcgt gtggtgcgaa tgcggtgatt cgaaaggatg gctgcgactt ctgcaccgcg
     1321 tgtggcgagg ttggcgcgtg tgggtgagca tcagctcacc actccgcagg cgcggtgaca
     1381 tacggcgcca acgcgaatca cgtggatgcg ctcgatagta cggccttaca aagggctatc
     1441 gcacagcgca atacggcgga ggatgcattg cgtgaggcgg gtgcaacgca gtgaagatgg
     1501 gagatgctca ggagaagatc gcttcgatat ccggcgacgc gcccttcacc tcgtcgaagt
     1561 tcaacgtcgc ctcgatatgc tccagatgtt ccagcatgca cgccaccgcc ttggctccgt
     1621 cgtgcgcttc gattgcatcg atcaaatcgc catgctcgtg atgcgggcac gccggtgcat
     1681 tcggtttgtc gtaaagcgtg atgacgaggc acgtcaaggg tgcgagttcg cgcatcatcc
     1741 gtaagagaat ctcgttgccg accatctccg cgatcagcac atgaaactca ccggacagcc
     1801 ggatgatggc gggccgatca ttctgtttgc gcgcatcgct ttcctgccgc acatgcttgc
     1861 gtaacgcgcg cacttgcgcc gatgtcgcgg tacggctcag cagcctggcc atttccggct
     1921 cgatcatgcg ccgcgtgaca aatacgtctc gcgcctgctg gacgctcggg ctggcgataa
     1981 aggctccgcg attcggcacc aactcgacca acccctcgtg ggcaagcttg ttgagcactt
     2041 cgcggatttt cgtccggctc acacctgaga cttccgcgag ccgctcctca atcaattttg
     2101 tgccgggtgc aaggcgatgc tcaagcaccg ccttctggat acgaccgtaa atttcctcgt
     2161 tcgtatacgg cgccttgggc ccggcaactt tactggctgg catggagaag gctcggctca
     2221 aagtgtttct cgcaactggg aagtgaatcg atgatagaac aatctgaagt gtttttgcac
     2281 acaaaaattc gatggaacgc tcgcatcggc ttctcgttca agcgaaggaa attgtaactt
     2341 attcgtatac aaaatctcat cggcgttgct gccgatgcgg ggcgccacgc accactttga
     2401 aaacagctcc ttgcgcgcgc gatactcgcg tttggtgcca acgattcgtc tttggtgcgt
     2461 ccacgcacca tctttatgca gtcgcgccat tcgtcagttt ataggttgaa ataacgacgt
     2521 ttgctccgcc gaaggcgctt gagaataggc ccatacgcta tccagcacgc ctccgtgatt
     2581 gcatacaata ttagttttat tgcgtacaaa ccgatcatgt tatgttggca tagagcttgc
     2641 tctagtagcg tcctcggtga gttcctgccg gccttgcgcc cacaactggc gcaaacgcct
     2701 cggccattga acccgcgcgt tgatctcacc gggtgctgcc acgctttgcc tcttccgccg
     2761 tttatcggga ggaagaaagg ctttgcgggg tcgtgcgtct ggtgcattcc gcgccgcttc
     2821 acccgctgtt ttatttcgta cgtattgtga tttccatgga gagcttgatg tccgaacgat
     2881 tttctcgtcg ccaattcctt caacgtgccg gcgcgggcgc agccctggtg tcgatcggtc
     2941 tgccccgttt tgcgatggcc gccgggctca cgcttgatgg catcaagaag gcgggcgtgc
     3001 tgcgcatcgg ctgcgaagcg acttatccac cgctgaccta tcgcgacgcg agcggcacca
     3061 tgatcggcta cgacgtcgac ctcgccaacg cgttttgccg caatctcggc gtcaaggcgc
     3121 agttcgtcga cacggtgtgg tcgggcgtga ttccgtctct ctatacgaag aagttcgatc
     3181 tggtgatgag cagcctgagc tatacggtcg agcgcgaaaa gcacgtggac ttctcggtgc
     3241 cttacgtgga agcttcgcag gcgctgctga ttcgcgcggc ggatcagaag agcgtcacgg
     3301 gcattgccgg catgaacggc aaggtgctgg gactcaagct cggctccccg ggtcagatac
     3361 tggagcccaa actcgacgcg caattgaaag cagcgggcgg cgcgggcttc aaggacgtga
     3421 aaaccttcga
//
LOCUS       DATASH010000169         1608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1803199,
            whole genome shotgun sequence.
ACCESSION   DATASH010000169 DATASH010000000
VERSION     DATASH010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1803199"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            346..618
                     /gene="rpsO"
                     /locus_tag="VHV99_04895"
     CDS             346..618
                     /gene="rpsO"
                     /locus_tag="VHV99_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX3634814.1"
                     /translation="MSAVETSKKSEVVAQFARAANDTGSPEVQVALLTTRINELTVHF
                     KAHAKDHHSRRGLLRMVSRRRKLLDYLKGKDADRYRSLIEKLGLRK"
     gene            984..>1608
                     /locus_tag="VHV99_04900"
     CDS             984..>1608
                     /locus_tag="VHV99_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX3634815.1"
                     /translation="MTMFNKIVKEFKWGQHNVRLETGEIARQASGAVIVDVEDTVVLA
                     TVVGAKTAKPGQDFFPLTVDYLEKTYAAGKIPGGFFRREGRPSEGETLISRLIDRPLR
                     PLFPEGFYNEVQVVIHVLSLNPEIPADIPALIGASAALAVSGLPFNGPVGAARVAYIN
                     NEFVLNPTRPQMKDSALDLIVAGTERAVLMVESEAQQLSEEVMLGAVV"
BASE COUNT          312 a          488 c          498 g          310 t
ORIGIN      
        1 ggcgtgcgtt tcgacaacga aggctccggc tacggctttc gcacggtgct ggcgctgccg
       61 cccgagcgca ccgtcttgcc caccgtctgc aagatgcagc ggccctgacg cgcttgcgcg
      121 cggtgcgtga tcgtacgaga tggcgtgcgg tgcgccgtgc gttacgcgcc acgcagcatc
      181 cagcccggca cagcggggtt cgccgccgga ttgggctgtg ctacaatacg cgtcccgttg
      241 tgaagcaggt tgtcatattt gtctggctgc ttgtatgtca cacggggtat tccacggcac
      301 ggcctttctt ccgtgaccgc ctgtctagtt cttaaggaaa tcgacatgtc cgcagttgaa
      361 acaagcaaga agtctgaagt cgttgcgcaa ttcgcacgcg cagctaacga caccggatcc
      421 cccgaagttc aggtcgcgct gctcacgacc cgcatcaacg aactgaccgt tcacttcaag
      481 gcacacgcga aagatcacca cagccgccgc ggtctgctgc gcatggtgag ccgtcgtcgc
      541 aagctgctcg actacctgaa gggcaaggac gcggatcgtt accgcagcct gatcgagaag
      601 ctgggtctgc gtaagtaatc ggccagtcgt tcagcaagat gcctgtgtca gttccactga
      661 tacaggcatt ttgtttttgc acggtgcgct tcatgtgacg cgcatcgcct gtgctccgca
      721 gtggcgtgcc agttcatgcg gcgggtgcag gaaagagcag tacttgcagt aacccaggca
      781 gtaacaaagc agtagagcag gcagcacaac agaaacacgg ccgggcctca gggcggagct
      841 ttgtgtcatt ccagcggttc gcgcgcgtac ccgatgtagg gcgtgattct ctggaatggc
      901 ataacactac tcttcccatg cggcgccggt ctggcgttgc cgcgccgctt ctggtccgcg
      961 cggcattacg atgaggaaaa gcaatgacta tgttcaataa gatcgtcaaa gagttcaagt
     1021 ggggacaaca taacgttcgc ctcgaaaccg gcgaaatcgc tcgtcaagca agcggcgcgg
     1081 tgatcgtcga cgtcgaagac accgtcgtgc tggcgaccgt ggtcggcgcc aagacggcca
     1141 agccgggtca ggacttcttc ccgctgaccg tcgactacct cgaaaagacc tatgccgccg
     1201 gcaagatccc gggtggcttc ttccgtcgcg aaggccgtcc gtcggaaggc gaaacgctga
     1261 tctcgcgcct gatcgaccgg ccgctgcgtc cgctgttccc ggaaggcttc tacaacgaag
     1321 tccaggtcgt aatccacgtc ctgtcgctga acccggaaat ccccgctgac atccccgcgc
     1381 tgatcggcgc gtcggcggcg ctggcggtgt ccggtctgcc gttcaacggc ccggtcggcg
     1441 cagcgcgcgt ggcctacatc aacaacgaat tcgtgctgaa cccgacgcgt ccgcaaatga
     1501 aggattcggc gctcgacctg atcgtcgccg gcacggaacg cgcggtgctg atggtggaat
     1561 cggaagcgca gcagttgagc gaggaagtga tgctcggcgc cgtggtgt
//
LOCUS       DATASH010000170         5156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2001137,
            whole genome shotgun sequence.
ACCESSION   DATASH010000170 DATASH010000000
VERSION     DATASH010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5156
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2001137"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            284..754
                     /locus_tag="VHV99_04905"
     CDS             284..754
                     /locus_tag="VHV99_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634816.1"
                     /translation="MYFWKRLFSSEYEQTLWKWSLKRIFPDKRIDRSRVATQLEHIYQ
                     TRNRVAHHEPVYGRRLFDTLAAVDFVVANLCVAGSDGSTPLRKMLRPEHATLENQAHA
                     LQIRIDAFAGPSHPCAKRGYSHSRASRTPLREHRRACADATHSFKPRSPDPPRS"
     gene            complement(720..1751)
                     /locus_tag="VHV99_04910"
     CDS             complement(720..1751)
                     /locus_tag="VHV99_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006413312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HEX3634817.1"
                     /translation="MAFNVTLRQSGRQFQVEQDEPILIAALRQGIGLPYGCKNGACGS
                     CKGTVVSGEIEQRQHSSSALSNDEKTRGMALFCCATACTDLEVDIREVAGVGDVQVKK
                     LPCRVNAIERKADDVVVLKLQLPANERLQYLAGQYLEFILKDGKRRSYSMASAPHTEG
                     PIELHLRHMPGGAFTDHVFNTMKERDILRFEAPLGTFFLREDSAKPIVLLASGTGFAP
                     LKAIIEHAVFKNLDRPMTLYWGARRKKDLYLLELAEQWAREIPNFKFVPVLSEPDAGD
                     AWTGRVGFVHRAVIEDLPDLSAYQVYACGAPVMVESAQRDFTQHHALPEDEFYADSFT
                     SAADLANAV"
     gene            1846..2919
                     /locus_tag="VHV99_04915"
     CDS             1846..2919
                     /locus_tag="VHV99_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007747544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3634818.1"
                     /translation="MKATRNLRRPRVLIVGCGDVGMRCVPLLRPRAHVFALTSHAGRS
                     AELRAAGVTPLVGDLDARRSLKRLAGLAPTVLHLAPPQKTGDDDRRTRALLATLSARR
                     DRALRAARGAVVPVGRLRRIRASWAESETANIVPDRVRWTAAPRAPMRLVYASTTGVY
                     GDCGGAWIDETRLTQASNARAKRRVSAEQQLRRATARGVVTASIARIPGIYAGNRLPL
                     ARLEKRTPALIDADDVYTNHIHADDLAAILVRLVTHGRPARVIHASDDTSLKMGEYFD
                     AVADAFGRERAPRITRADAEQQMEPTLLSFMRESRRLVNRRLKEELRIRLRYPSVEDF
                     LREVREVHGRAGSAMHGQIETDG"
     gene            complement(3053..3241)
                     /locus_tag="VHV99_04920"
     CDS             complement(3053..3241)
                     /locus_tag="VHV99_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011546000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634819.1"
                     /translation="MVIHDTIGISSVMIAFICLGLALVGGLLVIMRLRHKYHPNLIGA
                     LIGAILCFLLLEALPALT"
     gene            complement(3410..4120)
                     /locus_tag="VHV99_04925"
     CDS             complement(3410..4120)
                     /locus_tag="VHV99_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HEX3634820.1"
                     /translation="MNLESILFTQGFGSRRQCRALIGDGRVSIGGSVCTDAEADLPTH
                     DGTFCFSVDGVVWPYREHAYLLLNKPAGYECSRDPQHHLSVFSLLPPQFAERGVQCVG
                     RLDQDTTGLLLLSDDGKFVHMFTSPKRKVPKRYLATTRHPIDDVQLRALRGGVLLHGE
                     PRPIVAVEAEARGEHALALTVMEGKYHQVKRMIAAAGNRCEALHRERIGGLALPATLA
                     EGAWQWLDETDLGSLRSG"
     gene            4215..>5156
                     /locus_tag="VHV99_04930"
     CDS             4215..>5156
                     /locus_tag="VHV99_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3634821.1"
                     /translation="MHVRSSSPPSSTRRLPPAFQRLAWSNLVAQSAEQISLAAAPLVA
                     VFALGAGARETGLLQTAQTLPFLLLSIPLGVWADRRSRRTLMALAESVRVVAMLCVLL
                     LVMTHTLSLPLLAALGFIAATGTVAYNVAAPSLVPSIVPREAYAQANGRLELARSVAY
                     SAGPALGGLLVGWIGAGWAYGCAAGLSALAVALLAGLREPPRAAASQRHFLLELRDGT
                     RFVLRDALLRPMLATAIFFNLGFFVLQAVYVPYAVHRLGLSASMVGVTLGAYGVGMVC
                     GALAAPAIARRLAFGRVLIIGPSCGLLASLVMVVTIVA"
BASE COUNT          897 a         1664 c         1648 g          947 t
ORIGIN      
        1 cgacctgacg cgccgtcctc ggtcggcagc ggcgcaccct gcatccagtc ggcgggcacc
       61 cgcgcctttt acatggaaag acgaggagcg aagcaagatc gaagcggcaa tccgcagcgc
      121 gcagcgcgcc gcctacgcca aactgagtcc gtccgagaag cgcgggctcg atgaggcggc
      181 gttccgtcgc ggtgtgccac caaggctgag tcacgcgttc aggctcgatg cgcggcagaa
      241 agcgatcgtt gtcccaggcg gacaggttat cacccaactc acgatgtact tctggaagcg
      301 gctgttttcc agcgagtacg agcagacatt atggaaatgg tcgctcaagc gcattttccc
      361 ggacaagcgg atcgaccggt cgagggtagc cactcaattg gagcatatct atcagacacg
      421 caatcgcgtc gcgcaccacg agccggtgta tggtcgcagg ttgttcgata cactggccgc
      481 ggttgatttt gttgtggcga atctatgtgt agccggcagc gacggatcca cgccattgcg
      541 caaaatgctt cggcccgagc atgcgacgct agaaaatcag gcgcacgcgt tgcagatcag
      601 aatcgacgct ttcgccggcc cgagccatcc atgcgcaaag cgcggctatt cacatagccg
      661 cgcttccaga actccgcttc gcgagcatcg acgcgcctgc gccgatgcca cgcactcctt
      721 caaaccgcgt tcgccagatc cgccgcgctc gtgaaggaat ccgcgtagaa ctcgtcttcc
      781 ggcagcgcat gatgctgggt gaaatcgcgc tgcgccgatt cgaccatcac cggtgcgccg
      841 cacgcgtaca cctgataagc cgacagatcc ggcaaatcct caatcaccgc gcgatgcacg
      901 aagccgacgc ggcccgtcca cgcatcgccc gcatccggtt ccgacagcac cggcacgaac
      961 ttgaagttcg gaatctcgcg agcccattgc tcggccagct cgagcaaata cagatccttc
     1021 ttgcggcgcg cgccccagta aagcgtcatc ggccggtcga ggttcttgaa caccgcgtgt
     1081 tcgatgatcg ccttcagcgg cgcgaagccc gtacccgagg caagcagcac gatcggctta
     1141 gccgaatctt cgcgcaggaa gaaggtgccg agcggggctt cgaagcgcag aatgtcgcgc
     1201 tctttcatcg tgttgaacac gtggtccgtg aatgcgccac cgggcatgtg acggaggtgt
     1261 aattcgatcg ggccttccgt gtgcggcgcg ctcgccattg aatagctgcg gcgcttgccg
     1321 tctttcagaa tgaattcgag gtactggccg gccagatatt gcagacgttc gttggcgggc
     1381 agttgcagct tcagcacgac gacgtcgtcg gccttgcgct cgatcgcgtt cacgcggcac
     1441 ggcagtttct tgacctgcac gtcgcccacg ccggccactt cgcggatatc cacttcgaga
     1501 tcggtgcatg ccgtcgcgca gcagaagagc gccatgccgc gcgtcttttc gtcgttcgac
     1561 aaggccgacg acgaatgctg acgctgttcg atctcaccgc tgaccaccgt gcccttgcac
     1621 gaaccgcagg cgccgttctt gcagccgtac ggcagaccga tgccctggcg caaggccgcg
     1681 atcaggatcg gttcgtcctg ttctacctga aactgccggc cgctttgccg gagcgtgacg
     1741 ttaaatgcca tagagtgttc gaaatcgaaa agtcggtaat cgttatcgga cccgctgcat
     1801 cgctgtgcgg tacctgccgc gcttgacggc tacaatgcgt ccactatgaa agcgacacga
     1861 aatttacgcc ggccgcgcgt gttgatagtt ggctgcggtg acgtcggcat gcgctgcgtg
     1921 cccttgttgc ggccccgcgc gcatgtcttc gcgctcacca gccacgccgg gcgcagcgcc
     1981 gaactgcgcg ccgctggcgt cacgccgctg gtcggcgacc tcgacgcgcg ccgcagtctg
     2041 aaacggcttg ccggccttgc gccgacggtg ctgcatctcg cgccgccgca aaagaccggc
     2101 gatgacgacc gccgtacccg tgccttgctc gctacgctga gcgcgcgccg cgatcgggca
     2161 ctgcgtgccg cgcgcggcgc ggtggtgccg gtcgggcggc tgcgccgcat tcgtgcctcg
     2221 tgggctgaat ctgagacagc caatattgta cccgacaggg tgcgctggac cgctgctccg
     2281 cgtgcgccca tgaggttggt gtacgcgagc acgacgggcg tctacggcga ttgcggcggc
     2341 gcatggatcg acgagacgcg cttaacgcag gcgtccaatg cacgcgctaa acgtcgcgtg
     2401 tcggccgagc agcaactgcg gcgcgccacc gcgcgaggcg tggttaccgc aagcatcgcg
     2461 cggattccag gcatttacgc gggcaaccgt ctgccgctcg cgcggctgga aaagcgcacg
     2521 ccggcgctga tcgacgccga cgacgtttac accaatcaca tccacgccga cgatcttgcc
     2581 gcgatcctcg tgcgcctcgt cacacacggt cggccggcgc gcgtgattca cgcgtctgac
     2641 gacacgtcgc tgaaaatggg cgagtacttc gatgcggtcg ccgacgcgtt cggccgcgag
     2701 cgcgcgccgc gcatcacgcg agccgacgcg gagcaacaga tggagccgac cttgctgtct
     2761 ttcatgcggg aatcgaggcg gctcgtgaac cggcgcctca aagaggaatt gcgcatacgc
     2821 ctgcggtatc cgagcgttga agactttttg cgcgaggtgc gcgaggtgca cggtcgggcc
     2881 ggtagcgcga tgcacggcca aatagagacg gacggctgag ccagcccagc gaatcgtcgc
     2941 tcgcgccacg ctgaccgcgg ccacggcatt ccgtgaaggg caacaacagt agttggctcg
     3001 accagaagga agagcgcctg aatcacccct cgccgcgcga gaacctggtg gcttacgtca
     3061 gtgccggcaa cgcctccagc aacaggaagc acagtatcgc gccaatcaaa gcgccgatca
     3121 gattcggatg atatttgtgc cgcagccgca tgatgaccag cagtccgccg accagggcga
     3181 gcccgagaca tatgaaagca atcatcacgg atgagatccc gatagtgtcg tggatgacaa
     3241 tggcgcccct ccttgaactc tttgtagtct ttgtttaatc gagtataggg cgagttgtaa
     3301 ggaacagcat gctgcgccgc agcgcgcgat cggacgcccc acagggctta tgtgcgggcg
     3361 cgtacacgat tctcctgatg catcaaggct ttaaaagggt tctaccggtt tacccgctcc
     3421 gtaaagatcc gaggtcggtt tcgtcgagcc attgccacgc tccttcggcg agcgtcgcgg
     3481 gcaatgcaag tccgccgatc cgctcgcgat gcagcgcttc gcaacgattg ccggctgccg
     3541 cgatcatccg cttgacctgg tgatatttgc cctccatcac ggtgagggcc agcgcgtgct
     3601 cgccgcgcgc ctcggcctcg acggcgacga tcggcctggg ttcgccatgc agcaagacac
     3661 cgccacgcaa ggcacgcaac tggacgtcgt cgatgggatg gcgcgtggtc gcgagatatc
     3721 gcttcggcac cttgcgtttc ggcgacgtga acatgtggac gaacttgccg tcgtcggaga
     3781 gcagcaggag gccggtggtg tcctgatcga ggcggcccac gcattgcacg ccgcgttcgg
     3841 cgaactgcgg cggcagcagg ctgaacacgc tcagatgatg ctgtggatcg cgcgaacatt
     3901 catagcctgc gggtttgttc agcagcagat aggcgtgctc gcgatacggc cagacgaccc
     3961 cgtccacact gaagcagaac gtgccgtcat gagttgggag atcggcctcg gcatccgtgc
     4021 agaccgaacc gccgatgctc acgcggccgt caccgatcag cgcccggcat tggcggcgcg
     4081 agccgaaacc ctgagtgaag agaatgcttt cgagattcat ggcgagcgaa gaataacacc
     4141 gcaagaagcg gagcgccgtg cagcgccata gtgaccacga tgggatttcc acgtctagtc
     4201 gcaggagttc atcgatgcat gtccgttcct cttcaccgcc ttcatccacg cgccgattgc
     4261 cgcccgcgtt ccagcggctc gcatggtcga atctggtcgc gcaatccgcc gagcagatca
     4321 gcctcgctgc cgcgccgctc gtcgccgttt tcgcgctcgg cgccggcgca cgcgagaccg
     4381 gtctgctgca aaccgcgcag acgctgccgt tcctgttgct gtcgattccg ctcggcgtct
     4441 gggctgaccg tcgctcgcgc cgcacgttga tggcgctcgc cgaaagcgtg cgcgtggtcg
     4501 ccatgctgtg cgtactgctg ctcgtgatga cgcacacgct gagcttgccg ctgctcgccg
     4561 cgctcggctt catcgccgcg accggtacgg ttgcctacaa cgtcgcggcg ccttcgctgg
     4621 tgccctcgat cgtgccacgc gaagcatatg cccaggccaa tggccggctc gaactggcgc
     4681 gcagcgtcgc gtattcggcg gggccggcgc tcggtggcct gctggtaggc tggatcggcg
     4741 cgggctgggc gtatggctgc gcggccgggc tatcggcgct ggccgtcgcg ttgcttgcgg
     4801 gcctgcgcga accgccgcga gcggctgctt cgcagcggca cttcctgctg gaactacgcg
     4861 acggcacgcg tttcgtgctg cgtgacgcgc tgctgcgtcc gatgctcgcc accgcgatct
     4921 tcttcaatct cggcttcttc gtgttgcaag cggtgtatgt gccgtacgcc gtgcatcggt
     4981 tgggcttgag cgcgtcgatg gtcggtgtga cgctcggcgc gtacggtgtc ggcatggtgt
     5041 gcggcgcgct cgccgcgccg gccatcgcgc ggcgcctggc gttcggccgc gtgctgatca
     5101 tcgggccgtc gtgcggtttg ctcgcctcgc tcgtgatggt ggtgacgatc gtcgcg
//
LOCUS       DATASH010000171         3122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4572936,
            whole genome shotgun sequence.
ACCESSION   DATASH010000171 DATASH010000000
VERSION     DATASH010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4572936"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1054
                     /locus_tag="VHV99_04935"
     CDS             <1..1054
                     /locus_tag="VHV99_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3634822.1"
                     /translation="IGVWAPILLTVLRVIQGVALGGEWGGGVLLAVEYAPKKYRGLFG
                     AVPQVGTMLGLALGSLAASEASAIFSKEDFLRVGWRIPFILSFVLVIIAFWIRRSVDE
                     TPSFKKVLASESASKVPLLETIKFHWRAVLMTVGAKFIETSVFYIFAVFSISYTIGLG
                     FERTDTLNAVLVASLLSAPMVLFSAWISDRIGRKKIFIIGALASMAFVLPFFWLVHQK
                     SIALLYLAVVVGFAVIYPMVGAVMGPLFAENFPPEVRYTGISLGYQLGTALVGGPLPL
                     IATALLVAFHGSYVPIALFVGLCGIVSLIAISFAKEGRGQDLDARRTVPPLGVGPALD
                     AAARPPRLKNPISDNG"
     gene            1276..1899
                     /locus_tag="VHV99_04940"
     CDS             1276..1899
                     /locus_tag="VHV99_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEX3634823.1"
                     /translation="MSYLSLSALPAGILFALVTSITPGPNNTMLLASGVNFGFRRTLP
                     HVSGISVGVVLLMLSVGIGLGEAFVHFPVLYTVLEVASVAYLLYLAWKIGTSGELKVR
                     NGERRPMKFHEAIAFQWVNPKAWMMVLTAATTIHLSESTSMNALAMAVVFYIIGFPCI
                     CLWAGFGTAMRRVLADSRRLRIFNVAMALLLVLSLYPIALKLLGDSA"
     gene            complement(1906..>3122)
                     /locus_tag="VHV99_04945"
     CDS             complement(1906..>3122)
                     /locus_tag="VHV99_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX3634824.1"
                     /translation="LVVARRGSGYRVAARAQPARVAPTDWQPPSLTATWLLSDVFADH
                     SVPIKAGGGWLPNEWINETGLQHALRAMSRVPAARLGDYGHPYGFAPLRERIAEQLDR
                     RGLPVDVSNVLLTQGATQGLDLIVRTLLRAGDVVIVEDPGYCNLLQILKLAGLVVHGV
                     PRTPAGVDTDVLEALVAQHQPKAIFVNTTLQNPTGATFGMSAAFRLLQIAERQRMWVI
                     EDDVSRELAPPGAPMFAAMEGLQRVLYVGGFSKTVTPGLRCGYVVAERAVLRELARTK
                     MAVGLTSSEAIERIVDKVLLEGRYARHVEAVNERLKLVHASVEERLDSLGLELFHRPR
                     AGLFLWARLPIEPERAGEVATAALTHGIWLAPGSYFRPDDAPSAWFRFNAPYSTDDAL
                     WRFIERVGQGVI"
BASE COUNT          541 a          980 c          947 g          654 t
ORIGIN      
        1 catcggagtc tgggctccta tccttttgac agtactgcgc gtcattcagg gagtcgccct
       61 cgggggggaa tggggcggag gcgtcctgct ggcggtggag tacgcgccca agaaataccg
      121 gggccttttc ggcgcggtcc cgcaagtcgg aaccatgttg ggacttgctt tgggttcgct
      181 ggcagcgtcg gaggcgagcg cgatcttctc gaaagaggat tttctcaggg tcggctggcg
      241 gattcctttc attctgtctt tcgtcctggt catcatcgct ttttggattc gccgctcggt
      301 tgatgaaacg ccgtccttca agaaggttct tgcgagcgag agcgcgagca aggtgccatt
      361 gctggaaacc atcaaattcc actggcgagc agtgctgatg acagtcggcg cgaagttcat
      421 cgaaacctcg gttttctata tcttcgccgt gttctcgatt tcgtatacca tcggcctcgg
      481 cttcgagcgg accgatacgc tcaacgcagt gttggttgcc tcgcttttgt ctgctccgat
      541 ggtcctcttc agcgcgtgga tctccgaccg aattggccgg aaaaagattt ttattatcgg
      601 cgcgctcgct agtatggcct tcgttctacc cttcttctgg ctcgttcatc aaaaatcgat
      661 cgcgttgctg tacctggccg tcgttgtggg cttcgccgtc atttatccca tggtcggcgc
      721 tgtaatgggt ccgctgtttg cagaaaattt tccgccagaa gttcgatata caggtatttc
      781 gctgggttac cagctcggaa ccgcactggt tggtggtcca ctcccactta tcgctacggc
      841 actgttggtt gccttccacg gttcctatgt cccgatcgcc cttttcgtag gtctctgcgg
      901 catcgtttcc ttgattgcca tctcattcgc gaaggaaggc cgcggccagg atctcgacgc
      961 cagaaggacc gttcctcctt tgggggtcgg gcctgcgctc gacgccgcag cacgtccgcc
     1021 acgcctcaag aatccaatat ccgataacgg atgagattga gtttgccccc gaaaagcgaa
     1081 ttcccttgcg gagcaggtta gctcaagcaa ggagaagaac gatactggct gtgacgtttt
     1141 aagcctgcac acgtccgtgc gggcgaagat ctcaccgcct gtaccggaca ccatcccaaa
     1201 aaccgtacag gtactgtacc ggcaaccgcc gctagaatgc tccaaaggtc ttctcgacca
     1261 ttaccggagt gtcccatgtc ctacctcagc ctcagcgcgc tgcccgccgg cattctgttc
     1321 gcgctcgtca ccagcatcac gcccgggccg aacaatacga tgttgctggc ctcgggtgtc
     1381 aacttcggct ttcgccgcac gttgccgcat gtctcgggga tcagtgtcgg cgttgtgctg
     1441 ttgatgttgt cggttgggat cggtctgggc gaagccttcg tgcattttcc cgtgctgtac
     1501 acggtgctcg aagtcgcgag cgttgcgtat ctgctgtatc tcgcgtggaa aatcggcacg
     1561 tcgggcgaac tgaaggtcag gaacggcgaa cgccggccca tgaagtttca tgaagccatt
     1621 gcattccagt gggtcaatcc gaaagcgtgg atgatggtgc tgaccgccgc gacaacgatc
     1681 cacctgagcg agagcaccag catgaatgct cttgcgatgg cggtggtctt ttacatcatc
     1741 gggtttccgt gtatctgctt gtgggccggt ttcggcaccg cgatgcggcg cgtgctggcg
     1801 gactccaggc ggctgcgcat tttcaatgtg gcgatggcgc tgctgctggt gctttcgctg
     1861 tatccgatcg cgctgaagct gctcggcgat tccgcgtaag cggatctaga tcacgccctg
     1921 cccgactcgc tcgatgaagc gccacaacgc gtcgtcggtc gagtatggcg cgttgaaacg
     1981 gaaccaggcg ctcggggcat cgtcgggacg gaagtaggag ccgggcgcga gccagatgcc
     2041 gtgcgtgagc gcggcggtcg ccacctcgcc ggcgcgctcg ggttcgatcg gcagccgcgc
     2101 ccacagaaac aacccggcgc gcggccggtg aaacagttcg agaccgagcg agtcgagccg
     2161 ctcttcgacg ctcgcgtgca cgagcttgag ccgctcgttc acggcttcga cgtgacgcgc
     2221 ataacgccct tcgagcagca ccttgtcgac gatccgctcg atcgcttccg acgacgtcaa
     2281 gcccaccgcc atcttcgtgc gcgccagttc gcgcaacacc gcgcgctcgg cgaccacgta
     2341 gccgcagcgc agccccggcg tcaccgtctt cgaaaaaccg ccgacgtaga gcacgcgctg
     2401 caagccttcc atcgcggcaa acatcggcgc gccgggcggc gccagttcgc ggctgacatc
     2461 gtcttcgatc acccacatac gctgccgctc ggcgatctgc aacagccgga acgccgccga
     2521 catgccgaag gtcgcaccgg tcggattctg cagcgtggtg ttgacgaaaa tcgccttcgg
     2581 ctggtgctgc gcgaccagcg cttcgagcac gtcggtatcg acgccggccg gcgtgcgcgg
     2641 cacgccatgc accaccagcc ccgccagttt gagaatctgc agcagattgc aataaccggg
     2701 atcttcgacg atcacgacgt cgcccgcgcg cagcaacgtg cgaacgatca gatcgagccc
     2761 ttgcgtcgcg ccttgcgtga gcagcacgtt ggagacgtcg accggtaatc cgcgccggtc
     2821 gagttgctcg gcgatccgct cgcgcaacgg cgcaaagccg tacggatgcc cgtagtcgcc
     2881 gagacgcgcc gccggcaccc ggctcattgc acgcagcgca tgctgcaatc ccgtctcgtt
     2941 gatccactcg ttcggcagcc agccgccgcc cgccttgatc ggcaccgaat ggtcggcgaa
     3001 cacgtcggac agcagccagg tcgcggtgag gctgggtggc tgccagtcgg tcggcgcgac
     3061 ccgcgcgggc tgcgcgcgtg ccgccacgcg ataaccggaa ccgcgccgcg ccaccacgag
     3121 gc
//
LOCUS       DATASH010000172         8713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1013039,
            whole genome shotgun sequence.
ACCESSION   DATASH010000172 DATASH010000000
VERSION     DATASH010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 8713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8713
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013039"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..943)
                     /locus_tag="VHV99_04950"
     CDS             complement(173..943)
                     /locus_tag="VHV99_04950"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008111903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HEX3634825.1"
                     /translation="MILNEIADKVCLVTGASRGIGAAVARGLGARGAKVMVHYRSGRA
                     QAEAVAADIEQQGGTARIVEGDIAQPGTVERLIQETVETFGRLDVLINNAGDQISRVG
                     IAEFPDELFERHMAINVRPVFAACRAAVRQFRSQKSGGNIINVSSVAARTGGGQGSAV
                     YAGAKAFMSTFSRSIAKEVAPEGIRVNVVSPGVIKTDLQDRVTSPEQLKATVTQIPMG
                     RIGDPEDCVGTFLYLCSDQLSGYVTGQVVEVNGGLLMP"
     gene            complement(960..1751)
                     /locus_tag="VHV99_04955"
     CDS             complement(960..1751)
                     /locus_tag="VHV99_04955"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3634826.1"
                     /translation="MSNFTLERDGDGPPAVFLHGFCQSSAYWEPTADRLAAAGIACLA
                     PDLPGFGASAGERGPYTMAALADSVARLLDARGLSRITLIGGSMGGVVAQQFVLRYPE
                     RVARLLLVATGAKGADPAAALAKADAMAAAPWDEAAIEPVVNGFFLRPPSAPDLARFR
                     SIALSAAHAAAVDAARSNALNNTLDQLASIRVPTLIIQGRHDRARTPEHGAEMQARIA
                     GAALAVIEDAGHTPQLEQPEKFHDVALPFMLAARVDQPVADRTAS"
     gene            complement(1751..3097)
                     /locus_tag="VHV99_04960"
     CDS             complement(1751..3097)
                     /locus_tag="VHV99_04960"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX3634827.1"
                     /translation="MSSSEDSYHALSRRRFLTMSALAGTGAALGGLGLMSPARAAEVS
                     VRFAGWAFEPQVVEQRLRQFMAENPDIKVNYSPLDMQLYSEKMIALFNAGSQPDTFYV
                     RDTDLGAWVDAGWLQPIDGLPKLDELNRDIFPFDRQALFYKGKQYGTPYYGDIYVYIY
                     DRAALDKAGVKAAPVTLDQLKNAALEVKKAGIAQYPILKGYKTNVDGLSEFWSMVLAS
                     GGHLFNPAMDPVFPNEDKTALTVLQWMVDAMHSWQILDPRGLELDDTQVRDAYLSGQG
                     IFTSNVGNVFPRSNNPQYSKRAGNIRMTRFPAVKDVGKGPMGWARLYGMSSQTKARDA
                     AWRLMYFLGGKDAKGQYSSAETWYLKYGVGYPFKPLDSDPQIAASQKQLGYEPAIIQQ
                     QMANARTRENIGTTWYSEWDRFTQQQIQAVLMRQITPIAALTASAQKAQQLKKEAS"
     gene            complement(3146..4045)
                     /locus_tag="VHV99_04965"
     CDS             complement(3146..4045)
                     /locus_tag="VHV99_04965"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX3634828.1"
                     /translation="MSALLNPLFTAAGRQSRRKVFLIVAGIAVAIYILAPFCWLLLTS
                     FMHERDALTVPTQWIPRHPTLEHYEMFFHPTGTSAIVGGRAAEEMLPGMLNSLIAALG
                     TAAVNLVLGTLAGYSLARLRFRGQGALLGVYLGSRMVPGIALIVPLYLTLKNLGMLDH
                     LSALIITYVTFTLPFTIWLLKNYFQTIPRSLEEAALMDGCSWFQMLVKVLLPVAMPGL
                     VSSAMFAFMTAWNDYLFAVILTSTTASKTLPVVVAGFATDVTTQRTLMATGGVLAIIP
                     PLLLAFLFQRLIVQGLTSGAVKD"
     gene            complement(4042..4980)
                     /locus_tag="VHV99_04970"
     CDS             complement(4042..4980)
                     /locus_tag="VHV99_04970"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX3634829.1"
                     /translation="MRLSLSVSSGNQRLRSKRVGYDRRAFAWLLNTPALLAIVLLSAF
                     PIAYSAWISLHRYNLQRPHAFKFVGLSNYSDILQSPEFWTALRITVVYTVLVVLLVVV
                     FGILIALLLNREFPGRALVRTLVLLPWAIPPVVNGLMWQWIYDAKVGALNGLLVSLGL
                     IDQYRGWLSTPTSALLALVFSEVWSLLPLAVILLLSALQRIPAELYEAAGMDGASRFQ
                     LFRYITLPWLSQSLLVVLILQTMSALRAFDVIYVLTAGGPGTATTTLTWQTYLTTFDN
                     LDFGHGNAYAWLVSLITLGLALAYFRVLYGRGDFEA"
     gene            complement(4977..6440)
                     /locus_tag="VHV99_04975"
     CDS             complement(4977..6440)
                     /locus_tag="VHV99_04975"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEX3634830.1"
                     /translation="MNGTPQAHSSPHWLFQGGFVIDGSGAPRFRADVRVENGRISEIG
                     ADLGLRGATCIDASGRVVAPGFIDVHTHDDESVLSAPQMLSKISQGVTTVVVGNCGIS
                     LAPLVHPNVPPPLTLLGGSGKHVFPTMKAYAQAVDGARPAVNVAALVGHSTLRVATMD
                     DPYRAATPAEQTKMADLMREAMAAGATGLSTGLFYDTNAAADVAEVTLLARIAAKAGG
                     IYTAHIRNEAEDVIASLDEAFTAGREAALPVVISHHKCAGPANWGRSVETLAHIDAAR
                     RTQPIGLDAYPYVAGSTILRPDLVDGIIEIMVTWSEPYPAMSGRSLADIAQEWQCSQK
                     EACERLQPGGASYFQMREDDVRRVLQHEATMIGSDGLPHDRHPHPRLWGTFPRVLGHY
                     SRELGLFPLEEAVHRMTGLSARRFNLLDRGEIAVGKHADLVVFDPDFVIDRATYAQPT
                     TPAAGIDSVMVSGSMTYDTHGATGIRAGRFLRLGARS"
     gene            complement(6826..7773)
                     /locus_tag="VHV99_04980"
     CDS             complement(6826..7773)
                     /locus_tag="VHV99_04980"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX3634831.1"
                     /translation="MDNLQVVPHSTTDTLARIRAISDSLPDAQQQVGNAILAEPEWSV
                     RANVAEIAHRAGVSAPTVVRFCRAVGFEGLSDFKLQLAQSLAVGTPYLHRAVNAKDDV
                     ASLVHKILYGAAAVLTGLESQLDADTIGHAIEKLAAANRIDCYSVGSVSTFLANDAQA
                     RFARLGLTSNAYFDPHMQVISAAALSPRDVVLAISHVGRMPTLLEAVALAREQGATVI
                     GITRPDTPLAKQCSIPIGMIVPEDAAARVGTEAYLAGQILIEVLMVGVGLRLGPSALE
                     RLKRVRSVLRERGVDSDVHPALQRAWSEAERDALGGKQV"
     gene            complement(8245..>8713)
                     /locus_tag="VHV99_04985"
     CDS             complement(8245..>8713)
                     /locus_tag="VHV99_04985"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX3634832.1"
                     /translation="MITQMTQHSDRAADRIIGVRAALQARGLQLRHEHLIEMPHKIIE
                     GQMAMKALMNGPERPTAVICGTDLLAIGALAEARESGIDVPGDLSIAGINDIEFSSFT
                     SPPLTTMRLAADEIGARSAEYLLARIEGRPVSSQYIVPTDLIVRGTTARPRNI"
BASE COUNT         1643 a         2707 c         2780 g         1583 t
ORIGIN      
        1 acttgccgca gccggaagga cccacgagca ccgcaaattc tccatcaggg atatccacgc
       61 tgatatcttg aatgacggag tgagagccat aggacttttt aacgttgtgg atgctgaggt
      121 ttgccaaagc gatgctccgg ggaatgagta gttgaatggc gggacgctcg cgtcaaggca
      181 tcagcagacc gccgttgacc tcgacgacct ggccggtcac gtagccgctc aactgatccg
      241 agcacaggta aaggaaggtt ccgacgcagt cctcgggatc gcctatacga cccatcggga
      301 tctgcgtcac ggtggccttc agttgttcgg gcgaggtgac gcgatcttgc aagtcggtct
      361 tgatcacccc gggcgagacg acattcaccc tgatgccttc gggtgcgact tcctttgcga
      421 tcgaacgtga aaatgtcgac atgaacgcct tcgcgccggc atacacggcc gagccttgac
      481 cgccgcccgt gcgtgccgcg acggaactga cgttgatgat attgccgccg ctcttctgac
      541 tgcggaactg gcgcacggcc gcgcggcatg cggcgaacac aggccgcacg ttgatcgcca
      601 tatggcgctc gaagagctca tcgggaaact cggcaatgcc gacgcgcgaa atctggtcgc
      661 cggcattgtt gatcagcacg tccaaccgcc cgaaggtctc gaccgtttcc tggatcagcc
      721 gctcgacggt gccgggctgc gcgatgtcgc cctccacgat gcgggcggta ccgccctgct
      781 gctcgatgtc ggcggccacg gcctcggcct gcgcgcgtcc gctgcggtaa tgaaccatca
      841 ccttcgcacc acgggcgccg aggccgcgtg ccaccgccgc gccaatcccg cggcttgcgc
      901 cggtcacgag gcagaccttg tccgcgattt cattcagaat catctacatt ctccgtggtt
      961 caggatgcgg tccggtcggc gaccggttga tcgacgcgtg cagcgagcat gaaaggcagc
     1021 gcaacgtcat gaaatttctc aggctgctcg agttgcggtg tgtggcctgc gtcctcgatg
     1081 acggccaacg cggcgcctgc aatccttgcc tgcatctccg cgccatgctc gggcgtgcgc
     1141 gcgcggtcgt gccgaccctg gatgatcagc gtcggaaccc ggatagaggc gagttggtcg
     1201 agcgtgttat tcaatgcatt ggagcgtgct gcatcgacgg cggcggcgtg cgccgcgctc
     1261 agcgcgatgc tgcggaacct cgccagatca ggagccgatg gcgggcgcag aaagaacccg
     1321 ttcacgacgg gctcgatcgc ggcttcgtcc cagggcgcgg ccgccatggc gtcggccttc
     1381 gcgagcgccg cggcggggtc cgcgcccttc gcgccggtcg cgacgagcag caggcgcgcg
     1441 acgcgctccg ggtagcgcaa tacgaactgc tgcgcgacga cgccgcccat tgacccgccg
     1501 atcaacgtga tgcgggacag gccgcgtgca tcgagcagac gcgcgaccga gtccgccagc
     1561 gcagccatcg tgtacggacc gcgttcgccg gccgatgcgc cgaagcctgg cagatcgggc
     1621 gcgagacacg caatgccggc ggcggcgagc cgatcggcag tcggctccca gtaggccgac
     1681 gactgacaga acccgtgcag gaaaaccgcc gggggaccgt ccccgtcgcg ctcaagcgtg
     1741 aaattgctca ttaactcgcc tccttcttca gttgttgcgc tttttgcgcc gacgcggtca
     1801 atgccgcgat cggcgtgatc tgccgcatta acacagcctg aatctgctgc tgtgtaaagc
     1861 ggtcccattc gctgtaccag gtcgtgccga tgttctcgcg agtacgcgca ttcgccattt
     1921 gctgctggat gatcgccggc tcgtagccca actgtttctg cgaggccgcg atctgtggat
     1981 cactatcgag cggcttgaac ggatacccca ccccgtactt gagataccac gtctcggcgg
     2041 atgagtactg gcccttggca tccttgccgc caaggaaata catcaggcgc caggccgcgt
     2101 cgcgcgcttt ggtctgggag ctcatgccat acaggcgtgc ccatcccatc ggtcccttgc
     2161 ctacatcttt cacggcggga aagcgcgtca tgcgaatgtt gccggcgcgc ttgctgtact
     2221 gcggattgtt ggagcgagga aagacgttgc cgacattgga cgtgaaaatg ccctggccgc
     2281 tcaggtaagc gtcgcgcacc tgggtgtcgt cgagttcgag gccacgcggg tccaggatct
     2341 gccagctatg catcgcgtcg accatccact gcagcaccgt tagcgccgtc ttgtcttcat
     2401 tgggaaacac ggggtccatc gcaggattga acagatggcc gccggaggcg agcaccatgg
     2461 accagaattc gctcagcccg tccacgttcg ttttgtagcc cttcaggatc gggtactggg
     2521 cgatgcctgc tttcttgact tccagggccg cgttcttgag ctggtcgagc gttaccggcg
     2581 ccgctttgac gccggccttg tcgagcgcgg cgcggtcgta gatgtagacg tagatgtcgc
     2641 cgtagtaggg tgtgccgtat tgcttgccct tgtagaagag cgcctgacgg tcgaaaggaa
     2701 agatgtcacg gttcaattcg tcgagcttcg gcaagccgtc gatcggctgc agccaacccg
     2761 cgtcaaccca cgcacccagg tccgtgtcgc gtacatagaa cgtgtccggc tgggagccgg
     2821 cattgaacag ggcgatcatc ttctcgctgt acaactgcat gtcgagcggc gagtagttca
     2881 ccttgatgtc gggattctcg gccatgaact gtcgcaatcg ctgttcgacc acctgcggct
     2941 cgaaggccca gcccgcgaag cgcaccgaca cttcagccgc gcgcgccggg gacattagcc
     3001 cgaggccgcc gagcgccgcg cccgtgccgg cgagcgcgga catcgtgaga aagcgacggc
     3061 gcgacaacgc atgataggaa tcttcactgg acgacatcgt ggtcctcctt gaggccatga
     3121 gattgcattg aatggattgc cataatcagt ccttcacggc accgcttgtc agcccctgga
     3181 cgatcaggcg ctggaacagg aacgccagca acagaggcgg gatgatggcc agtacgccgc
     3241 ccgtcgccat cagcgtgcgc tgggtcgtca cgtcggtggc gaagcccgcg acgactaccg
     3301 gcaatgtctt cgacgctgtc gtgctcgtca ggatcacggc gaacaggtag tcgttccacg
     3361 cggtcatgaa ggcaaacatc gccgaagaga cgagtccggg catggcgacg ggcagcagca
     3421 ccttgacgag catctgaaac cagctgcacc cgtccatcag cgcggcttct tcgagcgagc
     3481 gcgggatagt ctgaaagtag ttcttcagca accagatcgt gaacggcagc gtgaaggtca
     3541 cataggtgat gatcagcgcg gacaggtggt ccagcatgcc gagattcttg agcgtcagat
     3601 acagcggcac aatcagcgcg atacccggga ccatgcgcga gccgagataa acaccgagaa
     3661 gcgcgccctg cccgcgaaaa cgcaaacgag cgaggctata gccggcgagc gtgccgagca
     3721 ccaggttgac cgcggccgta ccgagcgcgg cgatcagcga gttcagcatg cccggcagca
     3781 tttcttcggc agcacggccg ccaacgatcg cgctcgttcc tgttggatga aagaacatct
     3841 cgtagtgctc gagcgtggga tgccggggaa tccactgcgt cggcactgtc agcgcatcac
     3901 gctcgtgcat gaaactcgtc agcagcagcc agcagaacgg cgcgaggata tagatcgcga
     3961 cggcgatacc cgccacgatc aggaacacct tgcgccgcga ttgacggccg gcagctgtga
     4021 acaacggatt cagaagagcg ctcatgcttc aaagtcccca cgtccgtaaa gcactcggaa
     4081 ataggccagg gcgagcccca gcgtaatcag gcttaccagc caggcgtagg cattcccgtg
     4141 gccgaaatcg aggttatcga acgtggtgag gtaggtctgc cacgtgagcg tggtggtggc
     4201 cgtgccgggg ccgcccgctg tcaggacata aatgacgtcg aaggcccgga gcgcggacat
     4261 cgtctgcaga atcaatacga cgagcagcga ctgcgaaagc cagggcaagg tgatgtaccg
     4321 gaacagctgg aacctgctgg cgccgtccat gccggccgcc tcgtaaagct ccgcgggaat
     4381 gcgctgcagc gcggagagca acaggatcac cgcgagcggc aacaggctcc agacttccga
     4441 gaacacgagc gcgagcaaag ccgaggtggg cgtcgacagc cagccgcgat actggtcgat
     4501 caagccgagg ctgaccagca ggccgttgag cgcgcccacc ttggcgtcat agatccattg
     4561 ccacatcagg ccattcacca cgggcggaat ggcccacggc agcaggacca gcgtgcgcac
     4621 cagcgcgcgg ccgggaaact cgcggttgag cagaagcgcg atcaggatgc cgaacaccac
     4681 caccagcaga accaccagca ccgtgtagac gacggtgatc cgcagggcgg tccagaactc
     4741 aggcgactga agtatgtcgc tgtagttcga caggccgacg aacttgaaag cgtgcggacg
     4801 ctggaggttg taccggtgca ggctgatcca cgccgagtag gcgatcggga atgcggaaag
     4861 caagacgatc gcgaggagcg ccggtgtatt gagcagccag gcgaacgcgc ggcgatcgta
     4921 gccgacacgt ttgctgcgaa gacgctggtt gccggacgat accgagagtg acagtctcat
     4981 gagcgggccc cgagacgcag gaatctgccg gcccgaatgc cggtagcgcc atgtgtgtcg
     5041 taagtcatcg agccgctcac catgacgctg tcgatgccgg cggccggcgt ggtcggctgc
     5101 gcgtaggtgg cgcggtcaat cacgaaatcc ggatcgaaca cgaccagatc cgcgtgcttg
     5161 ccaaccgcga tctcgccgcg atccagcaga ttgaaacggc gtgctgacag gccggtcatg
     5221 cgatgcacgg cctcctccag cgggaacaaa cccagctcgc gggagtaatg gcctaacacg
     5281 cgcgggaaag tcccccacag gcggggatgc ggatgacgat cgtgcggcag gccgtctgag
     5341 ccgatcatcg tcgcctcgtg ttgcaggacg cggcgcacgt cgtcctcacg catctggaag
     5401 taacttgcgc cgcccggctg caggcgctcg caagcctctt tctggctgca ttgccactcc
     5461 tgcgcgatgt cggccagcga gcgcccgctc attgcggggt aaggctcgga ccacgtcacc
     5521 atgatttcga tgatgccgtc caccaggtcc gggcgcaaaa tggtcgaacc ggcgacgtaa
     5581 gggtacgcat cgaggccgat cggctgcgtg cgccgcgcgg cgtcgatgtg tgcgagggtt
     5641 tcaacggacc gtccccagtt ggccggcccc gcgcacttgt gatgtgagat caccacgggc
     5701 agagcggcct ctcgcccggc cgtgaacgct tcgtccaggg acgcgatcac gtcctcggct
     5761 tcgttgcgga tatgcgcggt gtagatgccg ccggctttgg cggcgatacg cgccagcagc
     5821 gtgacctcgg caacgtccgc tgccgcgttg gtgtcgtaga agagacccgt gctgagcccc
     5881 gtcgcgcccg cggccatcgc ttcgcgcatc aggtccgcca tctttgtctg ttccgcgggc
     5941 gtcgcggcgc ggtaagggtc gtccattgtc gcgacgcgca atgtcgagtg gccgacgagg
     6001 gccgcgacgt tgaccgccgg acgtgcgcca tcgaccgctt gcgcgtaggc cttcatcgtt
     6061 ggaaagacgt gcttgccgga accgcccagc aaggtcagcg gcggcggcac gttcgggtgc
     6121 acgagcggcg cgagactgat cccgcaattg cccaccacca cggtggtcac gccctggctt
     6181 atcttggaca gcatctgcgg cgccgacagg acgctttcgt cgtcgtgggt gtgcacatca
     6241 ataaagccgg gcgcgacgac gcgcccggac gcgtcgatgc acgtcgcccc gcgcagcccg
     6301 agatccgcgc cgatttcact gatgcggccg ttttcgacgc gcacgtcggc gcgaaagcgg
     6361 ggtgctccgc taccgtcgat cacgaagccg ccctgaaaca gccaatgcgg ggagctgtgc
     6421 gcttgcggtg ttccgttcat aggtccaatt tcgccaaggc aaatgatgct gtggatgaga
     6481 tgtaagtaaa tttcactttc aaggcccgat atcgtcgcta tgcgccgcca aaaaagaacc
     6541 acatccgctc ggctgacgcc tagcattcaa cagacccagc acaaactcac tgcaatttcg
     6601 ctgtttacag cattaaatta atttgtattc ctattgaaat ggagcaaacc cgtttcggtc
     6661 cggctttacc cgctccccta tggcgattcg gccttggtgt aactaaattt cataaccaag
     6721 cgtaaagctt tggtcctttg atgtcaaaaa ggaaaaaaat aaatttttgg gggcgctcgc
     6781 cgatttcggc cgggggaccc gcgcggggct attccgccgg ggtactcata cctgtttgcc
     6841 gccgagcgca tcgcgctccg cttctgacca ggcgcgctgc aacgccgggt ggacgtcgct
     6901 atcgacgccc ctctcgcgca gcacggaccg gactcgcttg aggcgctcca gcgccgatgg
     6961 gccgagtcgc aagccaaccc cgaccatcag aacctcgatg agtatctggc cggcaaggta
     7021 cgcctccgtg cccacacgcg cggcggcgtc ctcgggcacg atcatgccga tcggaatact
     7081 gcactgcttc gcaagcggtg tgtcgggccg tgtaatgccg atgaccgtcg cgccctgttc
     7141 gcgcgcgagc gctacggcct ccagcaaggt cggcattcgt ccgacgtgtg aaatcgcgag
     7201 cacgacgtcg cggggcgaca gcgcggctgc cgagatgacc tgcatatgcg gatcgaaata
     7261 ggcgttcgat gtgagaccga ggcgcgcgaa gcgcgcctgg gcgtcgttgg cgagaaaagt
     7321 cgacacactg cccacgctat agcagtcgat ccggttggcc gcagcgagct tctcgatggc
     7381 gtgaccgatc gtgtcggcat cgagctggct ctcgaggccg gtcagcacgg cagcggcgcc
     7441 gtacaggatt ttgtggacga ggctcgctac gtcatctttg gcgttgaccg ctcgatgaag
     7501 gtacggcgtg cctaccgcca ggctctgtgc aagctgcagc ttgaagtcgc tcaacccttc
     7561 gaaacccacg gcgcggcaga atcgaacgac ggttggtgcg ctgaccccgg cgcggtgcgc
     7621 aatctccgcg acattggctc gcaccgacca ctccggctcg gcgagtatcg cgtttcccac
     7681 ctgttgctgc gcgtcaggca gggagtcaga aatcgcccgg attctcgcca gagtgtcggt
     7741 cgtggagtga ggtactacct gtaggttatc catgcttcag ttcgctgatt ttgcgcgcac
     7801 gtttgatgcc ccgcacgcag ttttccgggc aacgttttta cgataaccag tgtaaacata
     7861 tttcacgcac gccagtaaaa atacttggac gcgtgcttta tgtgcggttt caaccgagcg
     7921 ttgcgacgtt gggagaaact agattacacg ctatcgtgta tcgagcttgc gaggcaggtc
     7981 gctaaaccag cgttctggct atggttctgc gacgaattcg aggcgctcac tgcggccgaa
     8041 atgaagcggg cggacgggtt gcgtgctgag tccgcgcgca tgcatgcgcg ccgttgtatt
     8101 gtggttggaa cgggcggggt ctgagtctgc ccggtttcac gcataggcaa cccggcgtcc
     8161 gccggcgcgc tacgatccct gctcccgcgc tgacgtggtg cggccttctg agtctttttg
     8221 cacgacgcgc cgcgcgtcgg cgacctagat gttgcgagga cgcgccgtcg tgccgcgcac
     8281 gatcaggtcg gtcggcacga tgtactgcga actcacgggc cgtccttcga tccgtgcgag
     8341 cagatactcg gcgctgcgcg cgccgatttc atcagcggcg agccgcatgg tcgtcagcgg
     8401 cggcgacgtg aagctggaaa attcgatgtc gttgatgccc gcgatcgaca ggtcgcccgg
     8461 cacgtcgata cccgactcgc gcgcctcggc cagcgcgccg attgcaagca gatcggtgcc
     8521 gcagatgacg gccgtgggac gctcggggcc gttcatcagc gccttcatcg ccatctggcc
     8581 ttcaatgatc ttgtgcggca tctcgatcag atgctcgtgg cgcagttgca gtccgcgcgc
     8641 ctggagcgcc gcgcgcacgc cgatgatacg atcggccgcg cgatcgctgt gctgcgtcat
     8701 ttgcgtaatc atg
//
LOCUS       DATASH010000173         7700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_616086,
            whole genome shotgun sequence.
ACCESSION   DATASH010000173 DATASH010000000
VERSION     DATASH010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7700
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616086"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VHV99_04990"
     CDS             complement(<1..239)
                     /locus_tag="VHV99_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEX3634833.1"
                     /translation="MNLLKSFISLLALINPVGAIPFFLSLTTDMSGSEKAGTIRIASI
                     SVFCVIAVTALLGQQIIAFFGISVGSLEVGGGIIM"
     gene            complement(345..932)
                     /gene="hisB"
                     /locus_tag="VHV99_04995"
     CDS             complement(345..932)
                     /gene="hisB"
                     /locus_tag="VHV99_04995"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006400582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEX3634834.1"
                     /translation="MRLAEVVRNTSETQIRVKINLDGTGQQKLATGVPFLDHMLDQIA
                     RHGLFDLDIEAHGDLHIDDHHTVEDTGITLGQAVAKAVGDKKGIRRYGHSYVPLDEAL
                     SRVVIDFSGRPGLEFHVPFTRARIGTFDVDLTIEFFRGFVNHAGVTLHIDNLRGINAH
                     HQVETVFKAFGRALRMAVELDERAAGQIPSTKGSL"
     gene            complement(1050..2120)
                     /gene="hisC"
                     /locus_tag="VHV99_05000"
     CDS             complement(1050..2120)
                     /gene="hisC"
                     /locus_tag="VHV99_05000"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX3634835.1"
                     /translation="MTTPQDIIRRDVLAMTSYPVPDATGYIKLDAMENPFPLPPLLAA
                     HLGEHLAGVALNRYPAPRPEALIEKIKRVMGVPAGCDVLLGNGSDEIISMMSMACAKP
                     GAKVLAPVPGFVMYQMSATLAHLDFIGVPLKPDFTLDTEAMLAAIAEHEPAIVYLAYP
                     NNPTGTLYDDADMERIIAAANKSLVVIDEAYQPFAQQSWLPRADAFDNVVVMRTVSKL
                     GLAGIRLGYLVGKPGWLTEFDKVRPPYNTNVLTQAAADFLLDHIDVLDAQAEQLREER
                     TKLAQAVAQLPGAEVFPSAGNFLLVRVPDASVMFETLLAARVLIKNVSKMHPLLVNCV
                     RLTVGSPEENAQLIAGLKLVLH"
     gene            complement(2299..3621)
                     /gene="hisD"
                     /locus_tag="VHV99_05005"
     CDS             complement(2299..3621)
                     /gene="hisD"
                     /locus_tag="VHV99_05005"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX3634836.1"
                     /translation="MSTKIRKLDSSAPGFQKSLHAVLAFEASEDEAIERSVAQILNDV
                     KARGDAAVLEYTKRFDRVEATSVEALELPMSELEAALENLEPKRRAALEAAAARVRGY
                     HEKQKIECGSHSWQYTEADGTVLGQKVTPLDRAGIYVPGGKAAYPSSVLMNAIPARVA
                     GVREIVMVVPTPDGVKNPLVLAAALLGGVDRVFTIGGAQAVGALAYGTETVPAVDKIC
                     GPGNAYVASAKRRVFGTVGIDMIAGPSEILVLCDGTTDPRWVAMDLFSQAEHDELAQS
                     ILLCPDDAFIARVHDAINELLPTMPRQDVIRASLEGRGALIKVRDMAEACAIANDIAP
                     EHLEISALEPHQWAQLIRHAGAIFLGRYTSESLGDYCAGPNHVLPTSRTARFSSPLGV
                     YDFFKRSSVIEVSAEGAQTLGEIAAELAYGEGLQAHARSAEYRMRQNG"
     gene            complement(3682..4377)
                     /gene="hisG"
                     /locus_tag="VHV99_05010"
     CDS             complement(3682..4377)
                     /gene="hisG"
                     /locus_tag="VHV99_05010"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEX3634837.1"
                     /translation="MSSMPQTSSSPAVNAPLTLALSKGRIFEETLPLLAAAGIEVTED
                     PETSRKLILPTTDANVRVIIVRATDVPTYVEYGAADFGVAGKDVLLEHGGGGLYQPVD
                     LEIARCRMSVAVAAGFDYANAVRQGARLRVATKYVETAREHFAAKGVHVDLIKLYGSM
                     ELAPLVGLADAIVDLVSSGNTLRANNLVEVEEIMQISSRLVVNQAALKLKRAALRPIL
                     DAFERASKAGTAA"
     gene            complement(4374..5636)
                     /gene="murA"
                     /locus_tag="VHV99_05015"
     CDS             complement(4374..5636)
                     /gene="murA"
                     /locus_tag="VHV99_05015"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012402131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX3634838.1"
                     /translation="MDKLIIEGGYPLSGEVVVSGAKNAALPILCAGLLSAEPVHLENV
                     PDLQDVRTMLKLLGQMGVRIESGDGRVSLDASKVDNLVAPYEMVKTMRASILVLGPLV
                     ARFGHARVSLPGGCAIGARPVDQHIKGLQAMGAEITIEHGFIEARAKRLKGARIVTDM
                     ITVTGTENLLMAAVLAEGETVIENAAREPEVGDLAHLLVEMGAKIEGIGTDRLVIQGV
                     DKLHGAKHTVIPDRIEAGTFLCAVAAAGGDVTLRKVRPLILEAVTEKLREAGVTVEEG
                     DDWMRVRMDKRPSAVTFRTSEYPAFPTDMQAQFMALNALADGTSQVVETIFENRFMHV
                     QELNRLGANITIDGNTALVTGVQKLSGAKVMATDLRASASLVIAALRAEGETLIDRIY
                     HLDRGYDRMETKLNAIGAKVRRVSGSQA"
     gene            complement(5748..5987)
                     /locus_tag="VHV99_05020"
     CDS             complement(5748..5987)
                     /locus_tag="VHV99_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006029348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990229 - iron-sulfur cluster assembly
                     complex [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HEX3634839.1"
                     /translation="MLPTPEQVKQYIAAGLACQHLEVEGDGQHFFATIVSPSFEGKRL
                     IQRHQLVYAALGDRMREEIHALSMKTLTPAEWQSA"
     gene            complement(6003..6767)
                     /locus_tag="VHV99_05025"
     CDS             complement(6003..6767)
                     /locus_tag="VHV99_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3634840.1"
                     /translation="MSGYSGFSTLFYKEILRFWKVAFQTVLAPIITALLYLTIFGHAL
                     RGHVEVYPGVEYTSFLIPGLVMMSVLQNAFANSSSSLIQSKITGNLVFVLLPPLSHYE
                     MFGAYVLAAVARGLSVGFGVFIVTIWFVPVSFSAPLYIILFAIFGAAILGTLGLIAGI
                     LAEKFDQLAAFQNFLIMPLTFLSGVFYSTHTLPPVWREVSRLNPFFYMIDGFRYGFFG
                     MSDINPLVSLAIVAGFFVVLALVAMRMLATGYKLRH"
     gene            complement(6764..7696)
                     /locus_tag="VHV99_05030"
     CDS             complement(6764..7696)
                     /locus_tag="VHV99_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3634841.1"
                     /translation="MSAIEIRNVKKRYKDLQALKGVSLTVEEGEFFGLLGPNGAGKTT
                     LISILAGLARADEGSIAVRGHDVVDDFRNARRALGVVPQELVFDPFFTVRETLRIQSG
                     YYGLRNNDAWIDEIMANLDLTDKADANMRALSGGMKRRVLVAQALVHRPPVIVLDEPT
                     AGVDVELRQTLWKFISRLNREGHTIVLTTHYLEEAESLCDRIAMLRRGEVVALERTST
                     LLQRFAGMQLFVRFAQGVLPAELRPLEAESGAANGNGRQHLLRLTSYDDVERILAQCR
                     AAGCTFEEIEVRKADLEDVFVQVMNGPEVIEGLA"
BASE COUNT         1436 a         2443 c         2382 g         1439 t
ORIGIN      
        1 aacatgatga tgccgccgcc cacttcaaga gagccgaccg aaatcccgaa gaacgcgatg
       61 atctgctgtc cgagcagcgc cgtcacggca atcacgcaga agaccgaaat ggacgcgatc
      121 cggatcgtgc cggccttttc cgagccggac atatcggtcg tcaggcttag gaaaaacggg
      181 atagcgccga ccggattgat cagcgcgagc aacgaaataa acgatttgag tagattcatc
      241 gcagcccgtc atggcacgta tcgacgcgga cggcccggac gtgctcaatc acgcttgaga
      301 gcacgtccgg cgtgtaccgg ctttccgcgt tatttagaag gcgtttagag gctgcccttg
      361 gtcgatggaa tctgccctgc cgcgcgctcg tccagctcga cggccatgcg cagcgcgcgt
      421 ccgaatgcct tgaagacggt ttccacctga tggtgcgcgt tgatgccgcg caaattatcg
      481 atgtgcagcg tcacgccggc atgattcaca aagccgcgga agaattcgat ggtgagatcg
      541 acgtcaaacg tgccgatgcg tgcgcgcgtg aacggcacgt ggaactccag gcccggacgg
      601 cccgaaaaat cgatcacgac gcgcgacagc gcctcgtcga gcggcacgta ggaatgacca
      661 tagcggcgaa tgcccttctt gtcgccgacg gcctttgcga cagcctggcc gagcgttatg
      721 ccggtgtctt cgaccgtgtg gtggtcgtcg atatgcaggt cgccatgcgc ttcaatgtcg
      781 aggtcaaaca ggccatgccg tgcgatctgg tcgagcatgt ggtccagaaa cggcacgccg
      841 gtggccagct tctgctgacc ggtgccgtcc aggttgatct tcacacggat ctgcgtttcg
      901 ctggtgttgc gaacgacttc cgcaaggcgc atggtaattc ctcgaatcta gctagaaagc
      961 gatagtagtg tgggatggga acacggcggg ccgcgcgcac tttccccggt tgaaggaagc
     1021 gcttacgcgg ccattcggca cgccgttgct cagtgcagca cgagtttcag tccggcgatc
     1081 aactgggcgt tttcttcggg cgaaccgacg gtcaaacgca cgcaattgac cagcaatgga
     1141 tgcattttac tcacgttttt gatcagaacc cgtgccgcca gcagggtttc gaacataacg
     1201 gatgcatcgg gcacccgcac cagcaggaaa ttgccggcgc tcgggaacac ttcggcgccc
     1261 ggcaattggg ccacggcctg cgcgagtttc gtgcgctctt cgcgcagttg ctcggcctgg
     1321 gcgtcgagca cgtcgatatg gtcgagcagg aaatcggcgg cagcttgcgt cagcacgttg
     1381 gtgttgtacg gcggacgcac cttgtcgaat tcggtgagcc agcccggctt gccgaccaga
     1441 taacccaggc ggatgccggc caggccgagc ttggagacgg tacgcatcac gacgacgttg
     1501 tcgaacgcat cggcacgcgg cagccagctt tgctgcgcga acggctggta cgcctcgtcg
     1561 atcaccacca ggctcttatt cgccgcggcg atgatgcgct ccatgtccgc gtcgtcatac
     1621 agcgtgccgg tcgggttgtt cggataggcc agatagacga tcgccggttc atgctcggcg
     1681 atcgccgcca gcattgcttc ggtgtcgagt gtgaaatcgg gcttcagcgg cacgccgatg
     1741 aaatccagat gcgccagcgt cgccgacatc tgatacatca cgaaacccgg cacgggtgcg
     1801 agcaccttgg cgcccggctt cgcgcacgcc atcgacatca tgctgatgat ttcatccgaa
     1861 ccgttgccga gcagcacgtc gcaacccgcg ggcacgccca tcacgcgctt gatcttttcg
     1921 atcagtgctt ccggacgcgg cgccgggtaa cggttcagcg cgacgccggc cagatgttcg
     1981 cccagatgcg cggccagcaa cggtggcagc ggaaacggat tttccattgc gtcgagcttg
     2041 atatagcccg tggcgtccgg aaccggatag ctcgtcatcg cgagcacgtc acggcggatg
     2101 atgtcttgag gtgtcgtcat aattcagcgg aacggtccgc aagggagatg ctggcctggc
     2161 atcggcgcca ggttggtctg tcttgtcgtt atttaacgcg aaaccgcgcg aaaaaccgtc
     2221 ggtgaagctc ggggcaccga ttcgatgccc gcctgctccc tcatcgggtt cgccgcattc
     2281 aggcgctggt gctcaccttc agccgttctg ccgcatgcgg tattcggcgc tgcgggcatg
     2341 cgcctgcagg ccttcgccat aggcgagttc ggcggcgatc tcgccgagcg tctgcgcgcc
     2401 ttccgcactg acctcgatca cgctcgaacg cttgaagaaa tcgtagacgc ccaaggggga
     2461 cgagaaccgt gcggtacgag acgtaggcag cacgtgattg ggccccgcgc agtagtcacc
     2521 gaggctttcg ctcgtatagc ggccaaggaa gatggcgccg gcatggcgga tcagctgggc
     2581 ccattgatgc ggttccagcg cggagatttc gaggtgttcc ggtgcgatgt cgttggcgat
     2641 cgcgcaggct tcggccatat cgcgcacctt gatcagcgcc ccgcggcctt cgagcgacgc
     2701 gcggatcacg tcctggcgcg gcatggtcgg cagcagttcg ttgatcgcgt catgcacgcg
     2761 ggcgatgaac gcgtcgtccg gacacagcag gatggattgc gcgagttcgt cgtgctcggc
     2821 ctgcgagaac aggtccatcg cgacccagcg cgggtcagtc gtgccgtcgc acagcaccag
     2881 gatttccgac ggcccggcga tcatgtcgat gccgaccgta ccgaacacgc ggcgcttggc
     2941 cgacgcgaca tacgcgttgc ccggaccgca gatcttgtcg actgcgggca ccgtttccgt
     3001 accgtatgcc aatgcgccta ccgcttgcgc gccgccgatc gtgaacacgc gatccacgcc
     3061 gcccagcaag gccgccgcca gcaccagcgg atttttcacg ccgtccggcg tgggtacgac
     3121 catgacgatt tcgcgcacgc cggccacgcg ggccggaatc gcgttcatca gcactgacga
     3181 cggatacgcc gccttgccgc ccggcacgta gatacccgcg cgatcgagcg gcgtgacttt
     3241 ctggccgagc acggtgccgt cggcttccgt gtactgccag ctatggctgc cgcactcgat
     3301 tttctgcttc tcgtggtaac cgcgcacacg cgccgccgcc gcttcgagcg ccgcgcggcg
     3361 cttcggctcg aggttttcga gcgccgcttc gagttcggac atcggcaact cgagcgcctc
     3421 gacgctcgtc gcctcgacac ggtcgaagcg cttcgtgtac tcgagcaccg cagcgtcgcc
     3481 gcgcgctttc acgtcgttca gaatctgcgc gacggagcgc tcgattgctt cgtcttcgct
     3541 cgcctcgaac gcgagcaccg cgtgcagcga cttctggaag ccgggagcgc tggaatcgag
     3601 tttgcgaatc ttggtagaca tacgggtatc cgtttcggta aggcgcgcta aagcgtggtc
     3661 aaacgagcgt ggtcagacta ttcaggccgc ggtgccggcc ttcgacgcgc gttcgaacgc
     3721 atcgaggatc ggccgcagcg cggcgcgctt gagcttcagc gccgcctggt tcacaacgag
     3781 gcgcgacgaa atctgcatga tctcctccac ctcgacaaga ttgttggcgc gcaaggtgtt
     3841 gcccgagctc accaggtcga cgatcgcgtc ggcaaggccg accagcgggg ccagttccat
     3901 cgaaccgtac agcttgatca ggtcgacgtg cacgcccttg gcggcaaaat gctcacgtgc
     3961 ggtttcaacg tacttggttg caacgcgtaa gcgggcgccc tggcgcaccg cgttcgcata
     4021 atcgaaaccc gcggccaccg cgaccgacat gcggcagcgc gcaatctcca ggtcgaccgg
     4081 ctgatacagc ccgccgccgc catgttcgag caacacgtct ttgccggcca cgccgaaatc
     4141 ggctgcgccg tattcgacgt aagtcggcac gtcggtcgcg cgcacgatga tcacgcgcac
     4201 gttcgcgtcc gtggtcggca gaatcagctt gcgcgaggtt tccgggtctt cggtgacttc
     4261 aatgccggcc gcggcgagca gcggcagggt ctcttcgaag atacgccctt tcgacaacgc
     4321 cagcgtaagc ggtgcgttca cggccggcga agacgacgtt tgcggcatcg agctcatgcc
     4381 tggctcccgg agacgcggcg cacctttgca ccgatggcgt tgagcttggt ctccatccgg
     4441 tcgtagccac gatccaggtg ataaatccgg tcgatcagcg tttcgccttc ggcgcgcaac
     4501 gcggcgatca cgagactcgc cgaagcacgc agatcggtcg ccatcacctt cgcgccggac
     4561 agcttctgga cgccggtcac gagcgcggtg ttgccgtcga tcgtgatgtt ggcgccgagg
     4621 cggttcaact cctgcacgtg catgaagcgg ttctcgaaga tcgtttcgac cacttgcgac
     4681 gtgccgtcgg cgagcgcgtt cagtgccatg aactgcgctt gcatgtcggt cgggaacgcc
     4741 gggtattcgg aggtgcggaa cgtgacggcg ctcggacgct tgtccatgcg cacgcgcatc
     4801 cagtcgtcgc cctcttcgac cgtgacgccg gcttcgcgca gcttttcggt cacggcttcg
     4861 aggatcagcg gacgcacttt acgcaaagtc acatcgccac cagcggccgc gactgcgcac
     4921 aggaacgtgc ccgcttcgat acgatccgga atcacggtat gtttcgcgcc gtgcagcttg
     4981 tcgacgccct ggatgaccag gcgatccgtg ccgatgcctt cgatcttcgc gcccatctcg
     5041 accagcagat gcgcgaggtc gccgacttcc ggctcacgcg ccgcgttctc gatcaccgtt
     5101 tcgccttcgg ccaacaccgc tgccatcagc aggttttccg tgccggtgac ggtgatcatg
     5161 tcggtcacga tgcgcgcacc cttcagacgc ttcgcgcgcg cttcgatgaa accgtgttcg
     5221 atcgtgatct ccgcgcccat ggcctgcagg cccttgatgt gctgatccac cggacgcgcg
     5281 ccaattgcgc agccgcccgg cagcgatacg cgagcgtgac cgaagcgtgc gaccagcggg
     5341 ccgagcacga ggatcgacgc acgcatggtc ttcaccatct cgtacggtgc gacgaggtta
     5401 tcgaccttcg acgcgtccag cgacacgcgc ccgtcaccgc tctcgatgcg cacgcccatc
     5461 tggccaagca gcttgagcat cgtgcgcacg tcctgcaagt cgggcacgtt ctccagatgc
     5521 accggctccg cgctgagcag acccgcgcac aggatcggca acgccgcgtt cttggcaccc
     5581 gagacgacga cttcacccga cagcgggtag ccaccttcga tgatgagttt atccatgccg
     5641 gtctcttcct gattgacccg gacttcggcc ggggctgctt tggctgtgtt cgacgcgccg
     5701 ctaccggcgt cgcgtccttc ttgagtaatt cgcactaaat ttccagatta cgcggactgc
     5761 cattcggcgg gcgtcagcgt cttcatgctg agcgcgtgga tttcctcgcg catgcggtcg
     5821 ccgagcgccg cgtacacgag ttgatggcgc tggatcagac gcttgccttc gaaactcggc
     5881 gaaacgatgg tcgcgaagaa atgctgaccg tcgccttcga cttcgagatg ctggcaagcg
     5941 agcccagccg caatgtattg cttgacctgt tcgggagtcg gcaacatgag agaagctcct
     6001 gatcagtggc gcagtttgta gccggtggcg agcatgcgca tcgccaccag tgccagcacc
     6061 acgaagaaac cggcaacgat cgcgaggctc acgagcggat tgatatccga catcccgaag
     6121 aaaccgtagc gaaagccgtc gatcatgtag aaaaagggat tgagccgcga cacttcgcgc
     6181 cataccggcg gcagcgtatg cgtcgagtag aacacgcccg agaggaacgt gagcggcata
     6241 atcagaaagt tttgaaatgc agcaagctga tcgaacttct cggccaggat gccggcgatc
     6301 aacccgagcg tgccgagaat cgccgcaccg aaaatcgcga aaagaatgat gtagagcggc
     6361 gcgctgaagc tgaccggcac gaaccagatc gtcacgatga acacgccgaa cccgaccgac
     6421 agaccgcgtg ccacagcggc cagcacgtat gcgccgaaca tctcatagtg cgacaaaggc
     6481 ggcagcagca cgaacaccag gttgccggtg atcttcgact ggatcagtga ggacgagcta
     6541 ttcgcaaatg cgttctgcaa cacgctcatc atcacgagac cgggaatcag gaaactcgtg
     6601 tactcgacgc ccggatagac ctcaacgtga ccgcgcaacg cgtggccgaa aatcgtcaga
     6661 taaaggagcg ccgtgatgat cggcgccagc acggtctgga acgccacttt ccagaatcgc
     6721 aggatttctt tgtaaaacag cgtgctgaaa ccactgtaac cgctcatgca agcccctcga
     6781 tcacttccgg accgttcatc acctgaacga atacgtcttc gaggtcggcc ttgcggacct
     6841 cgatttcttc gaatgtacaa ccggccgcgc ggcattgcgc gaggatgcgc tcgacgtcgt
     6901 catagctcgt cagacgcagc agatgctggc ggccgttacc gttggccgcg ccgctttccg
     6961 cctcgagcgg acgcagctcg gcgggcagca ccccctgcgc gaaccgcacg aacagctgca
     7021 tgccggcgaa acgctgcagc agtgtactcg tgcgctcgag cgcgacgacc tcgccacggc
     7081 gcagcatcgc gatacggtcg cacagcgatt cggcttcttc caggtagtgc gtggtcagca
     7141 cgatggtgtg gccttcgcga ttcaggcgcg agatgaattt ccacagcgtc tggcgcagtt
     7201 cgacgtcgac gcccgcggtc ggctcgtcga gcacgatcac cggcggacgg tgcaccagcg
     7261 cctgcgcgac cagcacgcgg cgcttcatgc cgcccgacag cgcgcgcata ttggcgtcgg
     7321 ctttgtcggt gagatcgaga ttggccatga tctcgtcgat ccacgcgtcg ttgttgcgca
     7381 gcccgtagta gcccgactgg atgcgcaagg tttcgcgcac cgtgaagaac ggatcgaata
     7441 cgagttcctg cggcaccacg ccgagcgcgc ggcgcgcgtt gcggaaatcg tcgacgacgt
     7501 cgtgaccgcg caccgcgatg ctgccttcgt cggcgcgcgc gagacccgca agtatgctga
     7561 tgagcgtcgt cttgcccgcg ccgttcggac cgagcagtcc gaagaactcg ccttcttcga
     7621 ccgtgaggct gacgcccttg agcgcctgca agtccttgta gcgcttcttg acgttacgaa
     7681 tttctatggc tgacatgact
//
LOCUS       DATASH010000174         2506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3781867,
            whole genome shotgun sequence.
ACCESSION   DATASH010000174 DATASH010000000
VERSION     DATASH010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3781867"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VHV99_05035"
     CDS             <1..508
                     /locus_tag="VHV99_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEX3634842.1"
                     /translation="DEAARRQVQRIGSTVIVLLACDRFCGYLWAGDSRLYLYREGQLR
                     QLTRDHSQVEQLKSLGVITDEEARHHPAQHMITRAVGATDVLELDDDAIEVADGDVFL
                     LCSDGLSNEISDADILAVLTSVACERASDELVELAIARGGRDNVTAVVVQAEDPYASD
                     KTLLNPAP"
     gene            complement(619..1977)
                     /locus_tag="VHV99_05040"
     CDS             complement(619..1977)
                     /locus_tag="VHV99_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX3634843.1"
                     /translation="MMKILDQVEEPSPAISYAGLIERIEILRSTLRWSSRLERADIDK
                     LLRQATSLRDEVMQLSHKERFVQAATAAEPAAPADQSRGARRKALIERSFIFEGTFGD
                     NPRLLESLEIAEKAAPTDLPVLIDGESGTGKELMAKVIHANGSRPDKPFISVNCGAIP
                     DNLLESELFGHRKGAFTGASNDRKGKFESAHTGTIFLDEIGELPLAGQVKLLRVLEAH
                     EIQRVGSDETIAVDTRIVAATNRNLRQLSEEGKFREDLFYRLSVIHVTLPPLRERRDE
                     IPLLIAWFGDEAAGTLKRRPVRLTPRLRDFLLNYAYPGNIRELRNVMYRVSCLAGEMA
                     DIDHLPVDMRPAAPGSAAALSNAATATGQEQISVANLMSLSDAKRAASDDAEKAFLER
                     GLREVSGTVAELARRIDMNRSHLQMLLKKHGLHSKDFRNRNAPSPGKNGADEHDEEDS
                     DS"
     gene            complement(1974..>2506)
                     /locus_tag="VHV99_05045"
     CDS             complement(1974..>2506)
                     /locus_tag="VHV99_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018423716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3634844.1"
                     /translation="DAGVIDKAYFRAQIGYVMQSNLLFSGTIAENIASGDDAPERRRI
                     EEVAKIADAHAFISKMPLGYEQIVGERGIGLSGGQIQRLCIARALYHDPRLLVFDEAT
                     SALDSQSESNILGNMHDILKGRTAVIIAHRLSTIMRADKILVLYEGAIVEQGRHDELI
                     QRGGMYYQLVQKQLSA"
BASE COUNT          478 a          796 c          730 g          502 t
ORIGIN      
        1 ggatgaagcc gcgcgccgcc aggtgcaacg gatcggcagc acggtgatcg tgctgctcgc
       61 ctgcgaccgt ttctgcggct acttgtgggc cggcgacagt cgcctttatc tgtatcgcga
      121 agggcaattg cggcaactca cacgcgatca cagccaggtg gaacaactca agtcgctcgg
      181 agtgattacc gacgaagagg cgcggcacca cccggcgcag cacatgatca cgcgcgcggt
      241 cggcgccacg gatgtgctcg aactcgatga cgatgcgatc gaagtcgcgg acggcgatgt
      301 cttcctgcta tgcagcgatg ggttgagcaa cgagatcagc gatgcggaca tactggcggt
      361 gctgacctcg gtggcctgtg agcgcgcgtc cgacgaactg gtcgagttgg ccatcgcgcg
      421 cggtggacgc gacaatgtca ccgcggtggt ggtgcaagcg gaagatccgt atgcgtcaga
      481 caaaaccttg ttgaacccgg cgccttgaaa gcgcgggctg gaaagcgctg ccttaaacaa
      541 gctattggta ctccaggcac ctcaaggaac gctgggcagt caaggcgccc gagagcaacc
      601 cgggtcacgc ggccttcctc aactatcgct gtcttcctca tcatgctcgt cagccccgtt
      661 tttgcccggg gatggcgcat tgcggttgcg aaaatccttc gaatgcaggc cgtgcttctt
      721 caggagcatt tgcaaatgcg agcggttcat atcgatccgc cgggccagtt ccgcaacggt
      781 gccgctcact tcgcgaaggc cacgctcaag gaaggctttt tccgcgtcat cgctcgcggc
      841 gcgcttcgca tcgcttagcg acatcagatt cgccacgctg atttgctcct gccccgtcgc
      901 agtggctgca ttggacaaag ccgccgccga ccctggcgcg gccggccgca tatccaccgg
      961 cagatgatcg atatccgcca tctcccctgc caggcacgag acgcgataca tcacgttgcg
     1021 caattcgcgg atattgcccg gatacgcata gttcagcaga aagtcccgca aacgcggcgt
     1081 caatctgacg ggcctgcgct ttagcgtacc cgcagcctca tcgccgaacc atgcaatcag
     1141 cagcggtatc tcatcgcgtc gctcgcgcaa cggcggcaac gtgacgtgaa tcacgctgag
     1201 acggtagaac aaatcctcgc ggaacttgcc ctcttcgctg agctgccgca gattgcgatt
     1261 ggtcgccgcg acaatacgcg tatcgacggc aatcgtttca tcggaaccca cgcgctgaat
     1321 ctcgtgtgct tccagcacgc gcagcagctt cacctgaccg gccagcggca actcgccgat
     1381 ttcatcgaga aagatcgtgc ctgtatgcgc gctttcgaat ttccccttgc ggtcattcga
     1441 ggcccccgta aacgccccct tgcgatgccc gaacaattcc gactccagca gattgtccgg
     1501 aatcgcgccg caattcacgg agataaacgg cttgtccggg cgcgagccat tcgcgtgaat
     1561 caccttcgcc atcagttcct tacccgttcc gctttcgccg tcgatcaaca ccggcaaatc
     1621 ggtcggcgcg gctttctcgg cgatttcgag cgattcgagc aaacgcggat tgtcgccgaa
     1681 tgttccctcg aaaatgaagc tgcgttcgat caaggctttt ctacgcgcac cacgcgattg
     1741 gtcggcaggt gcagcgggtt cggcggccgt cgcggcctgc acgaagcgct ccttgtgcga
     1801 gagttgcatg acctcgtcgc gcaacgaggt ggcttgccgc agcagtttgt cgatgtcggc
     1861 acgttcgagc cgggacgacc agcgcagcgt cgagcgcaaa atctcgatcc gctcgatcag
     1921 acccgcgtac gaaatcgccg ggctgggttc ctctacctgg tcgagtatct tcatcacgcg
     1981 ctcaactgtt tctggaccag ctggtagtac atgccgccgc gctggatcag ttcatcgtga
     2041 cggccctgtt cgacaatcgc cccttcatac aacaccagaa tcttgtccgc gcgcatgatg
     2101 gtgctgagcc gatgcgcaat gatcaccgcc gtgcggccct tcagaatgtc gtgcatgttg
     2161 ccgagaatat tgctctccga ttgcgagtcc agcgccgagg tcgcctcatc gaataccagc
     2221 agacgcggat cgtgatacag cgcccgcgcg atacacagcc gctggatctg cccgccggat
     2281 aggccgatgc cgcgttcgcc cacaatctgt tcgtagccga gcggcatctt gctgatgaac
     2341 gcgtgcgcgt cggcaatctt cgcgacttcc tcgatacggc gccgttcggg cgcgtcgtcg
     2401 ccgctcgcaa tgttctcggc aatcgtgccg gagaacagca gattgctttg catcacgtag
     2461 ccgatctgcg cgcggaagta ggctttgtcg atcacgccgg catcgt
//
LOCUS       DATASH010000175         5140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2990229,
            whole genome shotgun sequence.
ACCESSION   DATASH010000175 DATASH010000000
VERSION     DATASH010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5140
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2990229"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(886..2175)
                     /locus_tag="VHV99_05050"
     CDS             complement(886..2175)
                     /locus_tag="VHV99_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX3634845.1"
                     /translation="MVFSKVLTKCAVTCATAICAVALAHADPLADTQAGPLTRAHVPR
                     IALDDDVYLPSAGLNEQIIRVPVDAAGTVTLETTIYKPDGPGPFPMIVFNHGKIPGDP
                     RTQERSDPLPFAREFVRRGYVVVAPNRQGFGHSDGTYQQDGCDVARNGVGQAGDVAAT
                     IDFMSKQPYVDATHIVVGGTSHGGLATMAYGPEAAPGVRALINFSGGLRQDACTDWQA
                     NLTRAFGAYGEKTKVPSLWLYGDNDSVWTAPLVAGMFAAYAAHGASAKMMDFGNYKND
                     AHRLVGDRDGVHVWWPAVEAFLAQAGMPTGVQYRVDDPSAPKASGFAAVDAVDSVPFV
                     DEAGRNGYRNFLHQYPSRAFAVSDSGAWSWAEGGDNPMSVAIANCQKQSSAPCRLYAV
                     DNSVVWGSGSSQTADSSSSSSSPSGSDNARRAIASRN"
     gene            complement(2759..3208)
                     /locus_tag="VHV99_05055"
     CDS             complement(2759..3208)
                     /locus_tag="VHV99_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HEX3634846.1"
                     /translation="MNKQLLSTQRPSDCPCGGAAPDQRNGAKPPRFEQCCGRYIDGGE
                     TAPRALELMRSRYSAYVVGATNYLRATWAPHTCPTDLDADPAAPDAPRWLGLQIKAFA
                     EIDADHATVEFVARYKVGGRAHRLHELSRFVRGEDARWRYVDGDVSE"
     gene            complement(3216..4346)
                     /locus_tag="VHV99_05060"
     CDS             complement(3216..4346)
                     /locus_tag="VHV99_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX3634847.1"
                     /translation="MVLDQDHLMVRDAVRTFVREAVTPHAAQWDRERTFPKDVHRQLA
                     ELGAYGVLVPEAYGGAGMDALALALILEEIAAGDGGTSTAISVNNCPVCSILLTYGND
                     AQKRDWLTPLARGEMLGAFCLTEPQAGSDASALRTTATRDGDSYVLNGVKQFITSGKN
                     GNVAIVMAVTDKAAGKRGISAFIVPTDTKGYVVARVEDKLGQHSSDTAQIIFEDCRVP
                     AANLIGAEGEGYRIALSGLEGGRIGIAAQSVGMARAAFEAALGYARERESFGQPLFSH
                     QAVQFRLADMATQLEAARQLIWHAASLKDAGQPCLTEAAMAKLFASEAAERICSAALQ
                     IHGGYGYLSDFPVERIYRDVRVCQIYEGTSDIQKILIARGLG"
     gene            complement(4383..5060)
                     /locus_tag="VHV99_05065"
     CDS             complement(4383..5060)
                     /locus_tag="VHV99_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014895885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3634848.1"
                     /translation="MKTVLIVGASRGIGQEFAHQYKKSGWRVLATARDGAALDALKTL
                     GAETFSLDVTAPEEIAALGWKLDGERLDAAVLVSGVYGPRTEGLETITAEDFDQVMHT
                     NVRGPMQLMPILLPLVEEAGGVLAVISSKMGSIGDASGTTGWLYRASKAALNDALKIA
                     SLEARRATCISLHPGWVRTDMGGAQAAIDPARSVTGMRDVLAQAAASHEAFNGRFYQY
                     DGTALDW"
BASE COUNT         1034 a         1622 c         1563 g          921 t
ORIGIN      
        1 ggttaagagg atgaaaagga cagtcgctca ggcttttctg cgggctcaag gcactgtagg
       61 cagacgagct gactgtccgt gtggcgacaa ctgatcgggt cggcgccacg gaagggaata
      121 tacaagtagg caaaagaaag cggctcacac cgccagctca taagcgggtc tcccgcacag
      181 tgacggtagt ggtgcatctg gaatctgtgc cctcgcacat tcggcgtgag tgacaaagga
      241 ttcgtcagca cccactccgc actgcgtgcg tcgcggatgg gtatgcgtgg gaaaccaggg
      301 gcttcgtttg gggcggtggg gaccatcggc ttcgcctcgg caaatgccga gtgaagcgtg
      361 agtgcaaaac cgtaatggtc ggttttacgg tttgggcgtg gggggccatt ggcttcgcct
      421 cggcaaattg cagaatgaag tgcgaaagcg agggcccagt ggtcggtttt atggagtgcg
      481 cttcggcgcg cgcagcgccg ccggaagtat gacggctgtg tcaccagggc cgaatgtgcg
      541 agggcacgga ttccagatgc accactaccg cgactgtgcg ggggacccgc ttatgagctg
      601 gcggggtgag ccgctttctt ttgcctactt ttatttgcgg caggcaaaga aaagtaggtg
      661 cccgccccgc acaggggcga cgctaataga ccgataacaa atcaaggaaa ggcccacgcc
      721 gcaggcatac agaaacaagc gccgcgcggg caaacaaatc aaggaaaggc ccacgccgca
      781 ggcatacaga aacaagcgcc gcgcgggcaa acaaatcaag aaaaggccaa cgccataaaa
      841 gcacagacaa acaaccgccg cgcaggcaaa aaaaagcctt aactgtcaat tccggctagc
      901 aatagcccgc cgcgcattat cagaccccga aggggaagag gaggaggaag aagaatccgc
      961 cgtctgcgaa gacccactcc cccatacaac ggagttatca acagcataca accggcaagg
     1021 cgccgaactc tgcttctggc aattagcaat agcaaccgac atcggattat caccaccttc
     1081 agcccatgac caggcacccg aatccgacac agcaaaagcc cggctaggat attgatgcaa
     1141 aaaattccga tacccattgc gaccagcttc atccacaaac ggcacagagt cgaccgcatc
     1201 gacagcagca aacccactcg ccttcggcgc cgaaggatca tcgacccgat attgcacgcc
     1261 agtaggcatc cccgcctgag caaggaaagc ctcgaccgca ggccaccaca catgcacgcc
     1321 atcgcgatcg cccacaagcc gatgcgcgtc attcttgtag ttgccgaaat ccatcatctt
     1381 cgcgctcgca ccgtgcgcgg catacgcggc aaacatgccg gccaccagcg gcgcggtcca
     1441 cactgaatcg ttatcgccat acaaccacag cgacggtacc ttggtcttct cgccatatgc
     1501 gccgaatgcg cgcgtcaggt tggcctgcca gtcggtgcac gcgtcctgcc gcaaaccgcc
     1561 cgaaaaattg atcaatgccc gcacgcccgg cgcagcctcg gggccgtaag ccatcgtcgc
     1621 aaggccaccg tgcgaagtgc cgcccaccac gatgtgcgtc gcgtccacgt atggctgctt
     1681 cgacatgaaa tcgatcgtcg cggccacgtc gccggcctgg ccgacgccgt tacgtgcgac
     1741 gtcgcaacca tcctgctgat aagtgccgtc ggaatggccg aagccctggc ggttcggcgc
     1801 caccaccacg tagccgcggc gcacgaattc gcgcgcgaac ggcagcgggt cgctgcgctc
     1861 ctgcgtgcgc gggtcgccag gaatcttgcc gtggttgaaa acaatcatcg gaaacgggcc
     1921 tggaccgtcc ggtttgtaga tcgtcgtttc gagcgtgacc gtgccggcag cgtcgaccgg
     1981 cacgcggatg atctgttcgt tgaggccggc gctggggaga tagacgtcgt cgtcgagtgc
     2041 gatgcgcggc acgtgcgcgc gagtgagagg ccccgcctgg gtgtcggcga gcgggtcggc
     2101 atgcgcaagt gcgacagcgc atatcgccgt tgcacacgtc accgcacact tcgtcagaac
     2161 cttgctgaac accattgacg cacctgcctc caaaacctgc cctgaacacc gtgaagcctt
     2221 gtctgtcgaa tgtcattgct gacaactgcc tttcggcgga gtttcgacgc acaaaaacac
     2281 actcgcgtac ggaatgcgcc aggcgcacgc acgctaatcg agatcgcacg ggatgaaccc
     2341 aaacggccga cgcgacccca cgcgccgatg atgagcgcca catctgctgc ccggaaccgc
     2401 cgtcgaaccc ttactggaag gctatcggca gggcgcctgc gcatttgcgg aagcggtatg
     2461 gaccggttct cgcaacgtga cgtcatgaac ctacggcctc atcctggcac gacaattttg
     2521 ttttgtgcaa tcaaggagaa cccgcgacga aaaaatctcc gtgtgtgaag cacgctacgt
     2581 tcgcccccgg aaagcggcaa aacgcccgtc gcgcggtgct tggctgcacg aagtaatttg
     2641 tcgcaacacg cgccctgtgg tgtcgaccca acacgaaaaa aggccctcgt ttgagggcca
     2701 tatgtgattg cggcgcgatc tgcgtcgttg agttgtgtaa ggctttggaa ggattgggtt
     2761 attcgctcac gtcaccatcg acatagcgcc aacgcgcgtc ctcgccgcgt acgaagcggc
     2821 tcaactcgtg cagccggtgg gcgcggccgc ctaccttgta tcgtgcgacg aattcgaccg
     2881 tggcatgatc ggcgtcaatt tcagcgaagg ccttgatctg gagtccgagc caacgcggcg
     2941 cgtcgggggc ggcagggtcc gcgtcgagat ccgtggggca ggtgtgcggt gcccaggtgg
     3001 cacgcaaata attggttgcg ccgacaacat aggcgctgta acgcgagcgc attagttcga
     3061 gcgcgcgcgg cgctgtttcg ccgccgtcga tgtagcgccc acagcattgt tcgaaacgcg
     3121 gtggtttggc gccgttgcgc tgatcaggcg cagcgccgcc gcaggggcag tcggatggtc
     3181 gttgcgttga caacagttgc ttattcatca gaccttcagc cgagaccgcg cgcgatcaag
     3241 attttctgga tgtcgctggt gccttcgtaa atctggcaga cgcgcacatc gcgatagatg
     3301 cgttcgacgg ggaagtcgct cagatagccg tagccgccat gaatctgcaa ggccgccgag
     3361 cagatgcgct ccgcggcttc ggaagcgaac agcttggcca tcgcggcttc cgtcaggcag
     3421 ggctggccgg cgtctttcaa tgaggccgcg tgccagatca actgacgcgc tgcttcgagt
     3481 tgggttgcca tatcggcgag acggaattgc acggcctggt gcgaaaacag cggctggccg
     3541 aaactctcgc gctccctggc atagcccaat gccgcctcga acgcggcgcg cgccatgccg
     3601 acgctttgcg ccgcgattcc gatgcgtccg ccttcgagcc ccgatagcgc aatccgatag
     3661 ccttcgcctt ccgcgccaat cagattcgcg gccggcacgc ggcaatcctc gaaaataatc
     3721 tgcgcggtgt ccgacgaatg ttggccaagc ttgtcttcga cgcgcgcaac gacgtaaccc
     3781 ttcgtatcgg tgggtacgat gaacgcgctg atgccgcgtt tgccggcggc cttgtcggtc
     3841 acggccatca cgatcgcgac gttgccgttc ttgccgctcg tgatgaactg tttgacgccg
     3901 ttcagcacgt agctgtcgcc atcgcgtgtc gcggtggtgc gcaacgcgga ggcgtcggag
     3961 cccgcttgcg gttcggtcag gcaaaacgca ccgagcatct cgccgcgcgc gagcggcgtg
     4021 agccagtcgc gtttctgcgc gtcgttgcca taggtcagca gaatgctgca caccgggcag
     4081 ttgttgacgg agatcgcggt cgaggtgccg ccgtcgcctg ctgcgatttc ctcgaggatc
     4141 agcgcgagcg ccagcgcgtc catgccggcg ccgccatacg cttcgggcac cagcacgccg
     4201 taggcgccga gttcggcgag ctggcgatgc acgtccttcg ggaaagtgcg ctcgcggtcc
     4261 cattgcgccg catgcggcgt aacggcttcg cgcacgaacg tgcgcaccgc atcgcggacc
     4321 atcagatgat cctgatcaag caccatggcc cagtctcctt gttagtctcg gcatttgtct
     4381 gctcaccagt cgagcgccgt gccgtcgtat tgatagaagc ggccgttgaa cgcctcatgc
     4441 gatgccgcgg cctgcgcgag cacatcacgc atgccggtga cgctgcgcgc cggatcgatc
     4501 gccgcttgcg cgccgcccat gtcggtgcgc acccagccag gatgcagcga aatacaggtt
     4561 gcgcgccgtg cttccagcga ggcgattttc agcgcgtcgt tcagcgcggc cttactcgcc
     4621 cggtacagcc acccggtcgt gccgcttgcg tcgccgatgc tgcccatctt gctcgatatc
     4681 accgccaaca caccgccggc ttcttcgacg agcggcagca ggatcggcat caactgcatc
     4741 ggaccgcgca cgttggtatg catgacctgg tcgaagtctt cggcggtgat cgtctcgagg
     4801 ccttcggtgc gcgggccgta tacgcccgat accagcaccg ccgcgtcgag ccgttcgccg
     4861 tcgagcttcc agccgagcgc ggcgatttcc tcgggtgcgg tgacgtcgag tgaaaaggtc
     4921 tccgcgccga gcgtcttcag cgcgtcgagc gccgcgccgt cgcgcgcggt ggcgagcacg
     4981 cgccagccgc tcttcttgta ctgatgcgca aactcctgac cgatgccgcg cgatgcaccg
     5041 acgatcaaca ctgtcttcat cgaatcacct caagccctta aaccctcaaa cgcctgcaaa
     5101 cagcgcgtta aaccagttca atgcccatcg ccgttgcttc
//
LOCUS       DATASH010000176         5446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_221388,
            whole genome shotgun sequence.
ACCESSION   DATASH010000176 DATASH010000000
VERSION     DATASH010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5446
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221388"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..451)
                     /locus_tag="VHV99_05070"
     CDS             complement(173..451)
                     /locus_tag="VHV99_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634849.1"
                     /translation="MAERVSGPYRGYYISAAARLVPAVEASAPAATTARGTYVGSVSL
                     AEQGPDDPHRMETLLELGDAQRFGSEEDALVFVEQAARDYVDRLLGGA"
     gene            685..876
                     /locus_tag="VHV99_05075"
     CDS             685..876
                     /locus_tag="VHV99_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634850.1"
                     /translation="MGKYFLQNHELPEPDAANTWFAYAENHGIDIPRAISIWEDAATE
                     SGTESRQKVSAAGITIETP"
     gene            941..1324
                     /locus_tag="VHV99_05080"
     CDS             941..1324
                     /locus_tag="VHV99_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634851.1"
                     /translation="MHFMTQLRRSGRFRPSSAALALVRGASLSVALPAAVLLGAGLAG
                     CASSNTTSLINLPNGQTGFAVNCSGADAASSWASCYVQAGKACGATGYDIVSKDNDEG
                     GTAGGSVTNVVSANVKNRSMIVRCK"
     gene            complement(1328..1666)
                     /locus_tag="VHV99_05085"
     CDS             complement(1328..1666)
                     /locus_tag="VHV99_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEX3634852.1"
                     /translation="MRMPPYALLAIAIVTEVIATSAMRASEGFSRLLPATVVVIGYGI
                     AFYCLSLTLKSIPVGIVYAVWSGAGIVLITLVAVVMYRQVPDVPAIIGLGLIIAGVAV
                     LNMFSKMQAH"
     gene            complement(1671..2168)
                     /locus_tag="VHV99_05090"
     CDS             complement(1671..2168)
                     /locus_tag="VHV99_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-degrading domain-containing protein"
                     /protein_id="HEX3634853.1"
                     /translation="MDIAHDLQVIAAQEHALVFPQFDADRAWQVGVYLHEVAKARGIG
                     AAIDVRTFGQPLFFSLLDGATPDNVDWARRKGNTVAHFRRSSYAIGLKMQQAGSTLAD
                     KHGLPATEYASHGGAFPLTVAGAGVIGSITVSGLPQRADHELVVEALCAHLGHDYSKL
                     ALAKA"
     gene            2568..2644
                     /locus_tag="VHV99_05095"
     tRNA            2568..2644
                     /locus_tag="VHV99_05095"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2602..2604,aa:Met,seq:cat)
     gene            3015..3560
                     /locus_tag="VHV99_05100"
     CDS             3015..3560
                     /locus_tag="VHV99_05100"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014897161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX3634854.1"
                     /translation="MKFCSVCGHGVSLSIPPGDNRERFVCGNCGTVHYQNPRNVVGTV
                     PVWDDKVLLCRRAIEPRYGYWTLPAGFMEMGETTAEAASRETLEEAGARVEVQSLFSL
                     LNVPHVHQVHLFYMARLLDLDIAAGEESLEVKLFEEHEIPWDEIAFPTIGQTLRFFFA
                     DRAAGSYGLHTGDIFRSLREG"
     gene            3570..4307
                     /gene="aat"
                     /locus_tag="VHV99_05105"
     CDS             3570..4307
                     /gene="aat"
                     /locus_tag="VHV99_05105"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEX3634855.1"
                     /translation="MVPWLGPDDPFPPVERALGVASGAPGLLAASGDLLPSRLIDAYR
                     RGIFPWYSDGQPVLWWSPDPRMILRPAEFKLSPSLRKTLKRVLREDAWEIRVDHDFAA
                     VMRACAQAPRRGQRGTWITADVVEAYSSLHRAGDAHSIETWFEGKRVGGLYGVSFGRM
                     FFGESMFAELADASKMALAALVGHLRRHEIEMIDCQQNTSHLASLGGREIARKAFIAH
                     VRASVDAPPIPWRFDKTVLLDVVAPAA"
     gene            4424..5245
                     /locus_tag="VHV99_05110"
     CDS             4424..5245
                     /locus_tag="VHV99_05110"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HEX3634856.1"
                     /translation="MTHPNELPLSPLSALQFYATAPYPCSYLDGRIARSQVATPSHLI
                     NSDVYTDLVKAGFRRSGVFTYRPYCDGCRACVPVRVPVDRFEANRTQRRVWKKHGELV
                     ATVAPLHYDEEHYALYMRYQSARHAGGGMDRDSRDQYEQFLLQSRINSRLVEFREPLP
                     DHPEAPGILRMISMIDILGDGLSSVYTFFEPGLPHTSFGTYNIYWQIEQAKSLKLPYV
                     YLGYWIRESPKMAYKANFRPLEGLIDGSWKVLDPACINLPPVDLAIQGRRGPLRP"
     gene            5332..>5446
                     /locus_tag="VHV99_05115"
     CDS             5332..>5446
                     /locus_tag="VHV99_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEX3634857.1"
                     /translation="MAGYHFPAARLRPVFSSLYPLVRAQLFRMDAEDAHHLT"
BASE COUNT         1061 a         1750 c         1589 g         1046 t
ORIGIN      
        1 gcaggcgcgt ccgcgggaaa ccgaatttcc atggtcgcct catcagaagt atccggcagg
       61 atagggacac cggtgagaac cggccacccc cggacatgcc cgatatggta ctcgcgcggc
      121 tggaccgcgg tccagcctcc agcgctgtcg tgctcaatgt cacgcgccgt catcaggcgc
      181 ctccgagcag gcggtcgaca tagtcgcgcg ccgcctgctc gacgaagacc agcgcgtcct
      241 cttcgctgcc aaagcgctgt gcatcgccga gttcgagcaa ggtttccatc cggtgcggat
      301 cgtccggccc ctgttcagcc aggctcactg aaccgacgta cgtacctctt gctgtggtgg
      361 cggccggcgc ggacgcttcg actgccggca cgaggcgcgc ggcggcgctg atgtaatagc
      421 cgcgataagg gccgctgacg cgttcagcca tgttcattct cctcgcttgc agttgatgga
      481 cgcacacctg atggacggtt cacacgcatg cgttcgcggc acacaggtaa gtaaggcgcg
      541 ccctgagccg ataagttcta tgcgcaaagc gatacgccgg attgcacttg tgccgctcgc
      601 ccctgtcgaa gcagttcacc ggcacattca ccggcacgat ccgtgctgac gctgtgacgc
      661 catttcgaat ccaacgagcg cagtatggga aaatattttc tgcagaacca cgaattgcct
      721 gagccggatg cggcaaacac atggtttgcg tacgccgaaa accacggcat cgacattccg
      781 agagccatca gcatctggga agacgcggcg accgaaagcg gcacggaaag ccgtcaaaag
      841 gttagcgcgg cgggcatcac gatcgaaacg ccttagcctt gagcgcacac ttccagtgta
      901 tgaggcgaga ctcgatatct atttgaaagg aaacatcacg atgcatttca tgacgcaact
      961 gcgccgctcc ggccgcttcc gacccagcag cgccgcgctt gcgctcgtcc gcggagcgtc
     1021 cctgtctgtt gccctgcccg ccgccgtatt gcttggcgcg ggtctcgccg gctgcgcatc
     1081 gtcgaacacc acatcgctga tcaatctgcc gaacggccag accggctttg cggtgaactg
     1141 cagcggcgcc gatgccgctt ccagctgggc ttcctgctac gtgcaggccg gcaaggcatg
     1201 tggcgcaacc ggctacgaca tcgtgtccaa agacaatgac gaaggcggca ccgcgggcgg
     1261 cagcgtcacg aacgtggtct cggccaacgt gaaaaaccgt tcgatgatcg tgcgttgcaa
     1321 gtaagactca gtgcgcctgc atcttcgaga acatattcag cacggcgacc ccggcaatga
     1381 tcagcccgag cccgatgatg gccggcacgt cgggcacttg ccggtacatc acgaccgcga
     1441 ccagcgtgat cagcacgatg ccggcgccgg accacactgc gtagacgatg ccgaccggaa
     1501 tactcttgag cgtcagcgac aggcaataga acgcaatgcc atagccgatc accacaaccg
     1561 ttgcgggcaa caaccgcgaa aaaccttccg acgcccgcat cgcggaggtc gcaatgactt
     1621 cggtgacgat cgcaatggca agtaaggcat aaggaggcat ccgcatcggc tcaggccttt
     1681 gcaagcgcga gcttgctgta gtcgtgaccg aggtgagcac acagtgcttc caccaccagt
     1741 tcatgatcgg cacgctgcgg cagtccggat accgtgatcg agccgatcac acccgcgccc
     1801 gctaccgtca atgggaaagc gccgccatga gacgcgtatt ccgtggccgg caagccgtgc
     1861 ttgtcagcga gcgtgctgcc cgcctgttgc atcttcaaac cgatcgcgta cgaactgcgc
     1921 cgaaaatgcg caactgtgtt gcccttgcgg cgcgcccagt cgacattgtc cggcgtcgct
     1981 ccgtcgagca agctgaagaa cagcggctga ccgaacgtgc gcacgtcgat agcggcgccg
     2041 ataccgcgtg ccttggcgac ctcgtgcaga tagacaccga cctgccatgc gcggtcggcg
     2101 tcgaattgcg ggaacaccag cgcgtgttcc tgagcggcaa tcacctgtag atcgtgagcg
     2161 atatccatgg agttcagagc aaggtaagaa gtgaagcgct ggccgttgca aatgcatacc
     2221 ggcgccagga agagaatctg aattctagcg cagcgcctta cgaaggcctt ccggtatgca
     2281 acgctggcaa tgcgcatggc tactttggaa gcagcagtgt tttggaagca gccgtgcttt
     2341 ggagttcgct gttttggagc tcgcctcggt ttggaagccg ctgcccttta gagttcgcct
     2401 cgctttggaa gccgctgcgc ttcaaagctc gctgcagcat tagaagctgc ttcgcgttga
     2461 aaaccactgc gcctgggagc cacctcgcat ggacgcatat tgaagtcggt tcttaaatag
     2521 ctattgcgcc ggccttctct ttgtcctata atcttttctt cgacggacgc ggggtggagc
     2581 agtctggcag ctcgtcgggc tcataacccg aaggtcgtag gttcaaatcc tacccccgca
     2641 accaagtcat ctctaagtag ctgaagcggt tcgaaacgtc gaaacaacat caaaggcttg
     2701 ccaaaccggc aggccttttt tgttttctgc ttcgcccttc attgctgccg tgcgtactct
     2761 gttttttcct cactcagata ctgcaggcct catgcgcatg caccaatctg gtagcaatcc
     2821 taactcaccc gccatcgaat agcgcggaaa agcgccagcg ttagccaatc ggacaacgcc
     2881 gccaccgccc gagccacgtc caccgcacca catgcgccgc tgccttcaca cccacgccgg
     2941 cctgccgtct tcgccgtccc tcgaaaacgc cagcccagtg ataaactccc atcactcttt
     3001 ctcgtcccct tcctatgaaa ttctgttctg tctgcggcca cggcgtcagc ctgagcattc
     3061 cgccgggtga caaccgcgag cgcttcgtat gcggcaattg cggcacggtg cactatcaga
     3121 atccgcgcaa cgtggtcggc accgttccgg tctgggacga caaagtgctg ctgtgccgtc
     3181 gtgcgatcga accgcgctac ggctactgga cgctgccggc gggtttcatg gaaatgggcg
     3241 aaaccaccgc ggaagcagct tcgcgcgaaa cgctggaaga agccggcgcg cgtgtcgagg
     3301 tgcaaagtct gttctcgctg ctgaacgtgc cgcacgtgca tcaggtgcat ctgttctata
     3361 tggcgcgcct gctcgacctc gacattgcag ccggcgagga aagcctcgaa gtgaaactct
     3421 tcgaggaaca cgaaattccg tgggacgaga ttgccttccc gaccatcggc cagaccttgc
     3481 gtttcttttt cgccgaccgt gccgccggca gttacggcct gcacacgggc gacatcttcc
     3541 gctcattgcg cgaaggttga gcggcgcgca tggttccctg gctcggacct gacgatccgt
     3601 ttcctcccgt cgagcgcgcg ctcggcgtgg caagcggcgc accgggcctg ctcgccgcca
     3661 gcggcgatct gctgccgtcg cggctgatcg acgcctatcg gcgtggcatc ttcccgtggt
     3721 actcggacgg ccagccggta ttgtggtgga gtcccgatcc acgcatgatc ctgcgccccg
     3781 ccgaattcaa actgtcgccc tcgctgcgca agacgctcaa gcgtgtgctg cgcgaggacg
     3841 cgtgggaaat ccgcgtcgac cacgatttcg ccgccgtgat gcgcgcctgt gcgcaagcgc
     3901 cgcggcgcgg ccagcgcggc acctggatca cggccgatgt ggtcgaagca tattcgtcac
     3961 tgcatcgggc aggcgacgcg cacagcatcg aaacctggtt cgaaggcaag cgcgtaggcg
     4021 gtttgtatgg ggtatcgttc ggccggatgt tcttcggcga atcgatgttc gcggagctcg
     4081 ccgatgcgtc gaaaatggcg ctggccgcgc ttgtcgggca cttgcgccgt cacgaaatag
     4141 aaatgataga ctgccagcag aacacgtcgc atctggcgtc gcttggcggc cgcgagatcg
     4201 cgcgcaaggc gttcatcgcg catgttcgcg cgtctgtcga cgcaccgccc attccctggc
     4261 gcttcgacaa gaccgtattg ctcgatgtcg tcgcgccggc ggcgtagtaa gaatcaggcc
     4321 gaatccggat ccgtcgccta ccgagtcgcg ggtcacgagc cgcgggttga aaaccgcaag
     4381 gcgccgggtt tgcaatacca gagacgcttc gagagctgcc aacgtgactc atcccaacga
     4441 gctgcctctt tctccgcttt ccgcgctgca attttatgca acggcgccct atccctgcag
     4501 ctatctggat ggacgcatcg cgcgctcgca agtcgccacg cccagccatc tgatcaactc
     4561 cgacgtctat accgacctcg tcaaagccgg cttccggcgc tcgggcgtgt ttacctaccg
     4621 cccgtattgc gatggctgcc gcgcctgcgt gcccgtgcgt gtaccggtcg accgtttcga
     4681 ggcaaaccgt acgcagcgcc gggtctggaa gaagcacggc gagctggtcg cgaccgtcgc
     4741 cccgctgcat tacgacgaag agcactacgc gctctacatg cgctatcagt cggcgcgaca
     4801 tgccggcggc ggcatggacc gcgacagccg cgaccaatac gaacagttcc tgctgcaaag
     4861 ccggatcaac tcgcggctgg tcgaattccg cgagccgttg ccggatcatc cggaagcgcc
     4921 aggcattctg cgcatgatca gcatgatcga catcctcggt gacgggctct cgtcggtgta
     4981 cacgttcttc gaaccgggtc tgccgcatac cagcttcggc acctacaaca tttactggca
     5041 gatcgagcag gcgaagagcc tcaagctgcc ctacgtgtac ctcggctact ggattcgcga
     5101 gagtccgaag atggcgtaca aggcgaattt ccggccgctc gaaggcttga tcgacggttc
     5161 atggaaggtg ctcgaccctg cctgcatcaa tctgccgccg gtcgatctgg cgattcaggg
     5221 caggcgcggg ccgctgcggc cttaacctgg caataacgta gccataacgt gcacaacctg
     5281 gccacatcag tccctcccag ttagtctcat taatgcccca aagccggtaa aatggcgggt
     5341 taccattttc cggccgcccg gcttcgtccc gtgttcagtt ccctctaccc gctcgtacgc
     5401 gcccaactct ttcgcatgga cgcggaagac gctcaccacc tgactt
//
LOCUS       DATASH010000177         4585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5365030,
            whole genome shotgun sequence.
ACCESSION   DATASH010000177 DATASH010000000
VERSION     DATASH010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4585
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5365030"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1711
                     /gene="glgX"
                     /locus_tag="VHV99_05120"
     CDS             <1..1711
                     /gene="glgX"
                     /locus_tag="VHV99_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEX3634858.1"
                     /translation="HVRGLSMLRPDLRQHERGTFAALASPEFIEHLLKLGVTAVELLP
                     VHAFLNDRFLVERGLRNYWGYNTAAFFAPEPSYLSTHRLDEMRIAVRQLHAAGIEVIL
                     DVVYNHTCEGNEMGPTVSWRGLDNASYYRLIPGDERHHINDTGCGNTVNLPHPRVLQM
                     VMDSLRYWSTKFNIDGFRFDLGVTLGREQHGFDPGSGFFDALRQDPILSQRKLISEPW
                     DIGPGGYQLGNHPPGFGEWNDRFRDTVRRFWRGDAGLRPDLAARLTGSADLFNRRFRK
                     PWASVNFVTSHDGFTLADVTAYEHKHNEANREDNNDGHNENCSRNWGVEGPTDAPVIH
                     ETRKRVARSLIATLLMALGTPMMLAGDESLRTQNGNNNAYCQDNELSWLDWERAESPD
                     GRQMTAFVARVIALRKQHPLLRETRFLFGDREVLPGLFDVGWFDAHGDPLTIEAWQDP
                     EGRAFTLRRAGPGLNGETEVLLMMLNAAEKTLRFTPPAPHLEWHVLLDTAEPESAPHR
                     LAVAEVEVAAHSLVMLAAQPVGEADWQAGWKAGAQHGPRLLTALPPDPGAHPPISDTS
                     PTT"
     gene            1834..3753
                     /gene="treZ"
                     /locus_tag="VHV99_05125"
     CDS             1834..3753
                     /gene="treZ"
                     /locus_tag="VHV99_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HEX3634859.1"
                     /translation="MSESPIDPHAHHHAHCLPFSAQLLGATGAKPRIRFRFWAPSCKT
                     VHVEVENGPAQGLHDMTPAGNGWFETTVDSGAGTLYRFRLDGEHAVPDPASRFQPQDV
                     HGPSEVIDPRAYRWEHTNWHGRPWEETVLYELHVGAMGGYAGVQKRLPALAALGVTAI
                     ELMPLNDFPGQHNWGYDGVLPYAPDSAYGRPEELKALIDAAHGLGLMVFLDVVYNHFG
                     PDGNYLHEYARSFFREGTHTPWGPAIDFDRSEVSDFFTDNAVYWINEYRIDGLRFDAV
                     HAIDNHAWLRELSDHIRAKVQHGRHVHLVLENEHNSASLLETHFDAQWNDDAHNTLHV
                     LLTGETEGYYHAYEDQPIRRLARVLSEGFAYQGDPSPIHDGAPRGESSRHLPPTSFVM
                     FLQNHDQVGNRAFGERLRKLTSDDALRAATGLLLLSPQIPLLFMDEEYGSTQPFLFFT
                     DYTGDLADAVREGRRREFARFSSFSDEKRRAQIPDPNDVKTFAASSPPEPVHGAATDD
                     EAKDRLNWMHFYKSALAVRAKLITPRLKHSKALGSTVLTAANGGDANALIARWKLCDG
                     ETLSVALNLSKENVAFPELPAGKVIFETPPRVREQIDAKVLPPHAFVAWVTGDVSDYA
                     IGHDARIAGQQERHA"
     gene            3750..>4585
                     /locus_tag="VHV99_05130"
     CDS             3750..>4585
                     /locus_tag="VHV99_05130"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013342041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEX3634860.1"
                     /translation="MSATRRSNDTIVTLATRAGFEVEWRDAHRKTQLVPESTLAVLLE
                     RMGLPCGNATQIRQSAAALEAELSGKKLPPLMTAEVERGIALPVAAIKSGSHYRIELE
                     SGSIIDGRFTAPKGEEALLAPIDEPGYHTLVINDQRVTLAVAPSRCYTVADAWRTLHD
                     GTTEQAPPLWGIAAQLYGLRRAGDGGIGDYSALARMAIESAKRGAHVLAVSPTHAMFS
                     AEPNRFSPYSPSSRWWLNVTHIDPAAVFGDDAARAALESAQAADPWSTLEDLPLIDWP
                     NAV"
BASE COUNT          873 a         1483 c         1434 g          795 t
ORIGIN      
        1 gcatgtgcgc ggcctgtcga tgttgcgccc cgatttgcgc cagcacgaac gcggcacgtt
       61 cgccgcgctg gcgtcgccgg aattcatcga acatttgctg aagctcggcg tgaccgccgt
      121 cgaattgctg cccgtgcacg cgtttctgaa cgaccgcttt ctggtggagc gcggcttgcg
      181 caactactgg ggttacaaca ccgcggcatt ttttgcaccg gagccgtcgt atctgagcac
      241 gcaccggctc gatgaaatgc gcattgccgt gcgccagttg cacgcggccg gcatcgaagt
      301 gattctcgac gtggtctaca accacacctg cgaaggcaac gagatgggac cgaccgtttc
      361 gtggcgcggc ctcgacaacg cgagctacta ccgcctgatc cccggcgacg aacgccacca
      421 tatcaacgac accggctgcg gcaacaccgt gaacctgccg catccgcgcg tgctgcagat
      481 ggtgatggat tcgctgcgtt actggtcgac gaagttcaat atcgacggct tccgtttcga
      541 tctcggcgtg acgctcggcc gcgagcagca tggtttcgac cccggctccg gttttttcga
      601 cgccttgcgc caggacccga tcctgtcgca gcgcaagctg atttccgaac catgggacat
      661 cggcccaggc ggttatcaac tcggcaatca tccgccgggc ttcggcgaat ggaacgaccg
      721 ctttcgcgat accgtccgcc gtttctggcg cggcgacgcg ggcctgcgcc ccgatctggc
      781 cgcgcgtctg acgggttcag cagatctgtt caaccggcgt ttcaggaagc cgtgggcatc
      841 ggtcaacttt gtcacgtcgc atgacggctt tacgctcgcc gacgtcaccg cttacgagca
      901 caagcacaac gaagcgaatc gcgaagacaa caacgacggc cacaatgaaa attgcagccg
      961 caattggggc gtagagggtc cgaccgacgc tcccgttatc cacgaaaccc gcaaacgcgt
     1021 ggcccgctcg ctgatcgcca cgctgctgat ggcgctcggc acgcccatga tgctggcagg
     1081 cgacgaatcg ctgcgcacgc aaaacggcaa caacaacgcg tactgccagg ataacgaact
     1141 atcatggctc gactgggagc gcgcggagtc gcccgatggg cgtcagatga ccgcgttcgt
     1201 cgcgcgcgtg atcgcgctgc gcaaacagca tccgctgctg cgcgaaacgc gctttctgtt
     1261 cggcgatcgc gaagtgttgc cgggcctgtt cgatgtcggc tggttcgatg cgcacggcga
     1321 tccgctcacc atcgaagcct ggcaggaccc cgagggccgc gcgttcacgc tgcggcgcgc
     1381 cggtccaggc ctgaatggcg aaacagaagt attgttgatg atgctcaacg cagcggaaaa
     1441 aacgctgcgt tttacgccac ccgcgccgca cctggaatgg catgtgctgc tggatactgc
     1501 tgagcccgaa agcgcgccgc accggttggc ggtggcagag gtggaagtgg ccgcgcatag
     1561 cctcgtgatg ctggcggcgc aacccgtcgg cgaagcggat tggcaggcgg gctggaaagc
     1621 cggtgcgcag cacgggccgc ggctgttgac cgcgctgccg cccgatcctg gtgcgcatcc
     1681 gccgatcagc gacacgtcgc cgaccacgta ggctgcgcat gaaagcacga cacgtgcagc
     1741 atggtaaggc agctgcatca cgacgcgtca ggcccccaga ggcgaacgac acgtgagacg
     1801 cggtgtgaaa gcaaattcga atggtgaaga atcatgtccg aaagtccgat tgacccgcac
     1861 gcgcatcatc acgcgcattg cctgccgttc agcgcgcagt tgctgggcgc taccggcgcg
     1921 aagccgcgta tccggttccg gttctgggcg ccatcgtgca agacagtcca cgtggaagtc
     1981 gaaaacggac cggcgcaagg cttgcatgac atgacgcccg ccggcaacgg ctggttcgaa
     2041 acgaccgtcg acagcggcgc gggtacgctg tatcgcttcc ggctcgatgg cgaacatgcg
     2101 gtgcccgatc ccgcttcgcg tttccagccg caagacgtgc acggcccgag cgaagtgatc
     2161 gacccgcgtg cgtatcgctg ggaacatacg aactggcatg gccgcccgtg ggaagaaacc
     2221 gtgttgtacg agttgcacgt cggcgcaatg ggcggctatg ccggggtgca gaaacgtttg
     2281 cccgcgcttg ccgcgctcgg cgtgaccgcc atcgaattga tgccgctgaa cgacttcccc
     2341 ggccagcaca attggggcta cgacggcgtg ctaccgtatg cgcccgactc cgcgtacggc
     2401 cgccccgagg aattgaaagc gctgatcgac gccgcgcacg gtctgggcct gatggtgttt
     2461 ctcgatgtgg tctacaacca cttcggcccg gacggcaact atctgcacga atacgcccgc
     2521 tcgtttttcc gcgagggcac gcatacgccg tggggtcctg cgatcgattt cgaccgcagc
     2581 gaagtgagcg atttcttcac ggacaacgcc gtgtactgga tcaatgaata tcgtatcgac
     2641 ggtctgcgtt tcgatgcggt ccatgccatc gacaatcacg cgtggctgcg cgaattgtcc
     2701 gaccatatcc gcgcgaaagt gcaacacggc cggcatgtgc atctggtgct ggaaaacgag
     2761 cacaacagcg cgagtctgct ggaaacgcat ttcgacgcgc aatggaacga cgacgcgcac
     2821 aacacgctgc acgttctgct gacgggcgaa acggaaggct attaccacgc gtatgaagac
     2881 cagccgatcc ggcgcctcgc gcgcgtgctg tcggaaggat tcgcgtatca gggcgatccg
     2941 tcgccgattc acgacggcgc accgcgtggc gaatcgagca ggcatctgcc gcccacttca
     3001 ttcgtgatgt tcttgcagaa tcacgatcag gtcggcaatc gcgcattcgg cgagcgcctg
     3061 cgcaagctga cctcggacga tgccttgcgc gccgccaccg gtttgctgct gctgtcgccg
     3121 caaatcccgc tgctgttcat ggacgaggaa tacggctcga cccagccgtt cctgttcttc
     3181 accgactaca ccggcgatct cgccgatgcc gtacgcgaag gacggcgccg cgaattcgcc
     3241 cgcttttcgt cgttcagcga cgaaaagcgg cgcgcgcaga ttcccgatcc gaacgatgtg
     3301 aagacgtttg cggcctcgtc accgcccgaa cctgttcacg gcgccgccac ggacgacgaa
     3361 gcgaaagacc gcctcaactg gatgcacttc tacaaatccg cgctcgccgt gcgggcgaag
     3421 ctgatcacgc cgcgcctgaa acattcgaaa gcactcggct cgaccgtgct gaccgccgca
     3481 aacggcggcg atgccaatgc gctgatcgcc cgctggaaac tctgcgacgg cgagaccttg
     3541 tcggtcgcgt tgaatctctc gaaggagaac gtagcgttcc ccgagctgcc tgccggcaaa
     3601 gtgattttcg aaacgccgcc gcgcgtgcgc gagcagatcg acgccaaggt gttgccaccg
     3661 cacgcgttcg tcgcctgggt gaccggcgac gtcagcgact atgccatcgg ccacgatgct
     3721 cgcatcgcgg gccaacagga gcgccacgcg tgagtgctac ccgacgttca aacgatacga
     3781 tcgtgaccct tgccacgcgc gccggtttcg aggtggagtg gcgcgacgcg caccgtaaga
     3841 cccagcttgt gccggagagc acgctcgccg tgctgctcga acggatgggc ttgccgtgcg
     3901 gtaacgctac gcaaatccgg caaagcgcgg ccgcgctcga agcggaactg tccggcaaga
     3961 aactgccgcc gctgatgacc gcggaggtcg agcgcggcat cgcgttgccc gtcgcggcga
     4021 tcaaatccgg cagccattat cgaatcgaac ttgaaagcgg ttcgatcatc gacggccgct
     4081 tcacggcgcc taaaggcgag gaagcattgc tcgcgccgat cgacgagccc ggctatcaca
     4141 cgctggtgat caacgatcaa cgcgtgacac tcgccgtcgc gccgtcacgc tgctataccg
     4201 tcgcggatgc atggcgcacg ctgcacgacg gcaccacgga gcaagcccct cccctgtggg
     4261 ggattgccgc gcagttgtac ggcttgcgcc gcgcgggcga cggcggcatc ggcgattatt
     4321 cggccctcgc gagaatggcg atcgaaagcg cgaagcgtgg ggcgcacgtg ctcgccgtca
     4381 gtccgacgca cgcgatgttc agcgctgagc cgaatcgctt cagtccgtat tcgccgtcat
     4441 cgcgctggtg gttgaacgtc acgcatatcg atcccgccgc ggtgttcggc gacgacgccg
     4501 cgcgcgccgc gctcgaaagc gctcaggccg ccgacccatg gtcgacactc gaagacttgc
     4561 cgctgatcga ctggcccaat gcagt
//
LOCUS       DATASH010000178         6120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2200280,
            whole genome shotgun sequence.
ACCESSION   DATASH010000178 DATASH010000000
VERSION     DATASH010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6120
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2200280"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VHV99_05135"
     CDS             complement(<1..312)
                     /locus_tag="VHV99_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006479459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HEX3634861.1"
                     /translation="MPLTRIALRAGKPAEYRKALTDSIQRSLVDTFNVPKDDIFMLIT
                     EHEAGNFVYDRQYLHIERSDDLVVIQITLNNTRTLDQKKALYKRMADELAESPGLRRE
                     DV"
     gene            414..1265
                     /locus_tag="VHV99_05140"
     CDS             414..1265
                     /locus_tag="VHV99_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007582631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX3634862.1"
                     /translation="MKVLDLDAVRAFVLVADLHSFTRAADALDTTQSAVSLKLKRLEA
                     HLGKQLVERTPRVVRLSADGSAFLGAARDLLSAHERALGSLSVERRRLALGLSEHVAG
                     TDLPALLARLNAHDPGLVVELHLGMSATLLAQFDERRLDAVIVRHGPDEPPRDDAQVL
                     FSEPLSWLATPEWLPRVGEPLALALLSPPCNVRDVALQTLNKAGVAWQEVFVGGGVAA
                     VGAAVAAGLAVSPLARRVAPRGLVDVGARLGLPQLPESRVSVHSRVRDKRSMETLRLV
                     TNGLATQ"
     gene            1355..2278
                     /locus_tag="VHV99_05145"
     CDS             1355..2278
                     /locus_tag="VHV99_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013691607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator ArgP"
                     /protein_id="HEX3634863.1"
                     /translation="MDLDPRQTAAVRAVIETGSFEQAAVRLNLTSSAVSQRVRALETR
                     LGNPLIVRTRPCRATQTGQRLLQYLRRTALLEDDFASDLAGANEALLNVAAAVNADTL
                     STWFFPALAEILLQENVLLDLTVDDQDHTHALLASGLAIGCITTEPAPMRGCFAERLG
                     AMRYRLVASAAFVERWFPHGLTRESARRAPVILSSRKDALQARFLETRFGLPPEAYPC
                     HYVPAPVPRYMAIQRGLAYGMVPELEFGDAVERGELVDLAPKQPTDVELYWHAWKVQS
                     PRLEKLSARIVELGRTALGPPGARVKRKRAA"
     gene            complement(2287..2916)
                     /locus_tag="VHV99_05150"
     CDS             complement(2287..2916)
                     /locus_tag="VHV99_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634864.1"
                     /translation="MAVAVWCVSGAAMAAGYSEVWNPPESTGHTAKHAKKTADAAKIK
                     SSASAKAGSKHAASGQHGAQRLALASKSGAKPAAHGSVKKVAAKNTGHGGVKKVAARN
                     ADHAGVAKGAGKPKSKLKATVVAQGKKPHAPLAQAKPGQGRVMHANLVRGHTAHAQVV
                     KVAEKSGEAKRAAVHTNAPANAVNVMASPADATANPATASSGSLPPILH"
     gene            3050..3487
                     /locus_tag="VHV99_05155"
     CDS             3050..3487
                     /locus_tag="VHV99_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbcA/ParS/Xre antitoxin family protein"
                     /protein_id="HEX3634865.1"
                     /translation="MAHAARLTQPEPPIRRPVSAPTLTGMSAAGLRAFFNIAQDWDLG
                     AEEQIVLLGSPGRSTYFKWKQAPETARLGRDTLERLSLLLGIYKALQILLPQPSAADT
                     WIKRPNSAPPFGGRRALDRMLAGNISDLVAVRQYLDAMRGGWA"
     gene            3484..4185
                     /locus_tag="VHV99_05160"
     CDS             3484..4185
                     /locus_tag="VHV99_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HEX3634866.1"
                     /translation="MTQPHWQDRWPTAPLDWSPAYRVIPTRFPAINLFDRVASPQDFD
                     ALYALEAMTNDRLRTEVGELDLVPREERRFGPGYGPIMAALTHLNPLGSRFSDGTYGV
                     FYCARSRATAIAETRYHTGKFLEATAEPPMRQQMRLYTVVAQGNVVDIRGDVSLDLAV
                     LSPDDYVAGQALGRAVREGGAPGIVYPSVRDAEGECLAAFRTTLLRDCNHAAYLEYNW
                     NGTSIDMVFELNQVG"
     gene            complement(4190..4927)
                     /locus_tag="VHV99_05165"
     CDS             complement(4190..4927)
                     /locus_tag="VHV99_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX3634867.1"
                     /translation="MASVLFNPVQHHPANRSGRDFVVGDLHGCVDALRYLLRDIAFDP
                     ARDRLFSVGDLVDRGEQSEQALALLDKPWFHAVLGNHEDTLCAVADGRLRRQWWYGIG
                     GIWAANLSDERLQHYAEQLRTLPLVRVVGSGKERFNVLHAEFFGTDAELDAGNFSAET
                     RQQLLWGRDLALGNGDPVRQHGLSLTYCGHTPMRDIQQFGSQVYIDTGAFGPGGRLTI
                     VEPQALRRWSISVEAAREAGAAAMALP"
     gene            complement(5076..>6120)
                     /locus_tag="VHV99_05170"
     CDS             complement(5076..>6120)
                     /locus_tag="VHV99_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX3634868.1"
                     /translation="DPDTGIGQWSAADFMRAMHEGVGKSGERLYPAFPYAEYTKVTDQ
                     DVLAIRAYLNTLTPIHYAPPGNSLKFPFNQRWLMVFWNLFNFDEGRFVPDPKQSAGWN
                     RGAYLVQGLAHCEECHTPRNFTQGLKTSSRFSGAVQAGWHAYNITSDKNSGVGNWSDD
                     ELVAYLSTGSAPGRANAAGPMADVVNDSTQYLTHEDVGSIVTYLRAVPPISGGESRPR
                     DQWGNPAVDDVTALRGTRVNAVNGAQLFIANCATCHNWTGQGVGASASGAYPSLIHNS
                     AVGASDANNLTMVILHGVSRKTKQADVLMPAFGTPLTDDQVAAITNYVTRQFGNPQST
                     VTVDQVARLRTQQ"
BASE COUNT         1059 a         2007 c         1891 g         1163 t
ORIGIN      
        1 cacgtcctct cgtcgcagcc ccggcgactc cgccagttca tccgccatcc gtttgtacag
       61 cgccttcttc tggtcgagcg tgcgcgtgtt gttcagcgtg atctgaatca ccacgagatc
      121 gtcacttcgt tcgatgtgca ggtactgcct gtcgtagaca aaattgccgg cctcatgctc
      181 ggtgatgagc atgaagatgt cgtctttcgg gacgttgaac gtgtctacca gcgagcgctg
      241 aatgctgtcc gtcagcgcct tccggtattc cgccggcttg ccggcgcgca atgcgattcg
      301 tgtgagcggc atggtgaaaa ctccttggtt gcggtgattg ggtaaacaca gcttaggcgc
      361 gctcagtcat aatgaacagc catcattggc tatttcatct atattcatca gaaatgaaag
      421 tcctcgatct cgatgccgtg cgcgctttcg tgctggttgc cgatctgcac agcttcacgc
      481 gggctgccga cgcgctcgac accacgcaat ccgccgtcag cctgaagctc aagcggctgg
      541 aggcgcatct cggcaagcag ttggtggaac gcacgccgcg cgtcgtacgt ctttccgcgg
      601 acggcagcgc tttcctcggc gcggcgcgcg atcttctgag cgcgcacgag cgggcgctgg
      661 gttcgctgtc ggtggagcgg cggcggctcg cgctgggact gagcgagcat gtcgcgggaa
      721 cagacctgcc ggcgttgctc gcgcggctca acgcgcacga tccgggtctg gtggtcgaat
      781 tgcatctggg catgtcggca acgctgctcg cgcaattcga cgaacggcgc ctcgacgcgg
      841 tgatcgtccg ccatggtccc gacgagccgc cgcgtgacga cgcgcaggtg ctgttcagcg
      901 aaccgctcag ctggctcgcc acaccggaat ggctgccgcg cgtcggcgag ccgttggcgc
      961 tcgcgctgct gagtcccccc tgcaatgtgc gcgacgtcgc gctgcaaacg ctgaataagg
     1021 cgggagttgc gtggcaggag gtgttcgtcg gcggtggtgt ggcggcggtc ggcgccgcgg
     1081 ttgcggccgg actggccgtg tcgccgctgg cgcgccgagt ggcgccgcgc ggcctggtgg
     1141 atgtcggcgc ccggctcggt ttgcctcagc ttccggaatc gcgcgtgagc gtgcattcgc
     1201 gggtgagaga taagcggtcg atggaaacgt tgcggctggt gacgaatggg ttggcaacgc
     1261 aatgacgaaa cgacttctca ctcgaaacta tgaataaagt tcgccggctt tcgcttaagc
     1321 tggaaaactg actatttcat ttaaaagtag atcagtggac ctcgaccccc gacaaaccgc
     1381 cgccgtgcgc gcggtgatcg aaaccggcag cttcgagcag gccgccgtgc gcctgaacct
     1441 cacctcgtcg gcggtatcgc agcgtgtgcg ggcactcgaa acccgcctgg gcaatccgct
     1501 gatcgtccgc acgcgcccgt gtcgcgcgac gcagacgggc cagcgtctgt tgcagtatct
     1561 acggcgcacg gccctgctcg aagacgattt cgccagcgat ctcgcgggcg ccaacgaagc
     1621 cttgctgaac gtcgccgccg ccgtgaacgc ggacaccttg tccacatggt tttttcccgc
     1681 gctcgccgaa atcctgctgc aggaaaacgt gctgctcgat ctgaccgtcg acgatcagga
     1741 tcacactcac gccctgctcg cctcaggact cgcgatcggc tgcatcacca ccgaaccggc
     1801 gccgatgcgc ggctgtttcg cggaacggct cggcgcgatg cgctaccggc tggtggcgtc
     1861 ggcagcgttc gtcgagcgat ggtttccgca tggattgacg cgtgaaagcg cgcggcgcgc
     1921 gccggtgatc ctctcctcac gcaaggacgc gttgcaggcg cgctttctcg aaacgcgctt
     1981 cggcctgccg ccggaagctt atccgtgcca ctacgtgccg gcgccggtgc cgcgttacat
     2041 ggcgatccag cgtggcctgg cttatggcat ggtgcccgaa ctggaattcg gcgatgccgt
     2101 tgagcgcggc gaactggtcg accttgcgcc gaagcagccc accgacgtcg agctgtactg
     2161 gcacgcctgg aaggtccagt cgccacggct cgaaaagctc tcggcgcgca ttgtcgaact
     2221 cggccgcact gccctgggtc cgccgggggc cagggtcaaa cgcaaacgcg ccgcctgagc
     2281 cggagcttag tggaggatcg gcggcaacga gccgctgctg gcggtagcgg gattggcggt
     2341 ggcgtcagcg ggactcgcca tcacgttcac ggcgttagca ggcgcgttcg tgtggactgc
     2401 cgcccgtttg gcctcgccgc ttttctccgc caccttgacc acctgcgcat gcgctgtgtg
     2461 gcctcgaacc aggttggcgt gcatgaccct gccttgaccc ggtttcgcct gagcgagtgg
     2521 tgcgtgcggc tttttaccct gcgcgactac agttgccttc aacttcgact taggcttgcc
     2581 cgcgcccttt gccacgccag cgtggtcagc gttcctagcc gcgactttct tcacgccacc
     2641 gtggcccgtg ttcttggccg cgaccttctt cacgctgccg tgcgctgccg gcttggcgcc
     2701 gctcttcgac gccaacgcaa gccgctgcgc gccatgttga ccgctcgccg cgtgcttcga
     2761 accggccttc gcgctggcgc tagacttgat cttggcggca tccgccgttt tctttgcgtg
     2821 cttcgccgta tgcccggtcg actcgggcgg attccacact tccgaataac cagcagccat
     2881 tgccgcaccg gacacacacc acactgcaac tgccattgct actactcgaa cccacatctc
     2941 cggcttctcc tgatcttcga ctccataacg agccgggatt atattctctt gcaaaaatcc
     3001 acttttagac taatatcaag ctaaagtaca gataaggact cacccactca tggctcacgc
     3061 tgcccgtctc acccagcccg aaccgcccat tcgccggccg gtttcggcac caacgctcac
     3121 gggaatgtcg gcggcaggcc tgcgcgcgtt cttcaacatc gcacaagact gggacttggg
     3181 cgccgaagag cagatcgtgt tgctcggctc gccgggccgc tccacttact tcaaatggaa
     3241 gcaggcgccg gaaacagccc gccttggtcg cgacacgctg gaacgcctgt cgctgctgct
     3301 cggtatttat aaggcgctgc aaatcctgct gccgcagccc agcgccgccg acacctggat
     3361 caagcgcccc aacagcgcgc cgccgttcgg cggccgccgc gcgctggacc gcatgctggc
     3421 cggcaacatc agcgacctcg tggccgtgcg ccagtatctc gacgcgatgc gaggcgggtg
     3481 ggcgtgacac aaccgcattg gcaggaccgc tggcccaccg cgccgctcga ctggtcgccc
     3541 gcgtatcgcg tgattcctac gcgctttccg gccatcaatc tgttcgaccg agtcgcatcg
     3601 ccgcaagatt tcgacgccct ctacgcgctc gaagcgatga ccaacgaccg cttgcgcacc
     3661 gaagtcggag aactcgatct ggtgccgcgc gaagagcgcc gcttcggccc gggatacgga
     3721 ccgatcatgg ccgcgttgac gcacctgaac ccgctcggca gccgcttctc ggacggcacg
     3781 tatggcgtgt tctattgcgc ccgttcgcgt gctacggcga tcgccgagac gcgctatcac
     3841 acggggaaat ttctcgaagc gaccgccgag ccgcccatgc ggcagcaaat gcggctgtac
     3901 accgtcgtgg cgcaaggcaa cgtggtcgat atccgcggcg acgtatcgct cgatctcgcg
     3961 gtgctgtcgc ccgacgacta cgtcgccggc caggcgctcg gccgtgccgt gcgcgagggc
     4021 ggtgcgccgg gcatcgtgta tccgtcggta cgggatgccg aaggcgagtg cctcgccgcg
     4081 ttcagaacca cgctgctgcg cgactgcaat cacgcggcct atctggaata caactggaac
     4141 ggcacgagca tcgatatggt gttcgagctc aatcaggtgg gctaggaggt caaggcagcg
     4201 ccatcgccgc cgctccggct tcccgcgccg cctcgaccga gatcgaccaa cgccgcagcg
     4261 cctgcggctc cacgatcgtc agcctgccgc cgggcccaaa tgcgccggtg tcgatataaa
     4321 cctgggaacc gaactgctgg atgtcgcgca tcggcgtatg gccgcagtag gtcagcgaaa
     4381 ggccatgctg acgcaccgga tcgccattgc ccaaggccag atcgcgcccc catagcaact
     4441 gctggcgggt ttccgcggag aaattgccgg cgtccaattc ggcgtccgtg ccgaaaaatt
     4501 cggcatgcag aacattgaag cgctccttgc cgctgccgac cacgcgcacg agcggcagcg
     4561 tgcgcagctg ttcggcgtag tgctgcaagc gttcgtcgga caaattcgcc gcccatattc
     4621 cgccgatgcc ataccaccac tggcgccgca agcgtccatc ggcgacggcg cataaggtgt
     4681 cttcgtgatt gccgagcacg gcatgaaacc atggcttgtc gagcaaggcc aaagcctgct
     4741 ccgactgttc gccgcggtcc accagatcgc cgaccgaaaa aagccggtcg cgcgccggat
     4801 cgaacgcgat gtcgcgcagc agatagcgca atgcatcgac acagccatgc aagtcgccga
     4861 cgacaaagtc gcggccgctc cgattggccg gatgatgctg aaccgggttg aagagtacgg
     4921 aagccattcg ctcatgatag tgcggcatcc gtcattgcgt atgcgcgctg cctatctttt
     4981 cctggtgtcc tgcagcatga gaaagacgta gaaacgacgc gaaaaacgcg cgcctccggc
     5041 aggacttacc ggaaaacgcg cgatcagcag agccgttatt gctgtgtgcg cagcctggcg
     5101 acctgatcca ccgttacagt ggactgcgga ttaccgaact gtctggtcac ataatttgtg
     5161 atagcggcca cctgatcgtc ggtgagcggg gtaccgaacg cgggcatcag cacgtcagcc
     5221 tgtttcgtct tacgactgac gccgtgcagg atcaccatcg tcagattatt ggcgtcgctc
     5281 gcgccgaccg ccgagttgtg aattagcgac ggatacgcgc ccgacgcgct cgcgcccacg
     5341 ccctgcccgg tccagttatg gcacgtcgcg caattggcga tgaagagttg cgcgccgttc
     5401 accgcgttga ccctggtgcc gcgcagcgcg gtcacgtcgt cgacggcggg attgccccac
     5461 tggtcgcgcg gacgcgattc accgccgctg atcggcggca ccgcgcgcag atacgtgacg
     5521 atcgagccga cgtcctcgtg cgtcagatac tgcgtgctgt cgttgaccac gtcagccatg
     5581 gggcccgctg cattggcgcg gcccggcgcc gaaccggtcg acagatacgc caccagttca
     5641 tcgtcgctcc agttacccac gccgctgttc ttgtccgacg tgatgttgta cgcatgccag
     5701 ccagcctgta ccgctccgga aaaacgcgag ctcgttttca aaccctgcgt gaaattgcgt
     5761 ggtgtatggc actcctcgca atgcgcgaga ccctggacca gatacgcgcc gcgattccac
     5821 ccggcgcttt gtttcggatc gggaacgaaa cggccttcgt cgaaattgaa cagattccag
     5881 aacaccatca gccagcgctg attgaacgga aatttcaggc tattgcccgg cggcgcatag
     5941 tggatcggcg tgagcgtgtt cagatacgcg cgaatcgcca gcacgtcctg atcggtgacc
     6001 ttggtgtact ccgcatatgg gaaggccgga tagagccgct cgccgctctt gccgacgcct
     6061 tcatgcatgg cgcgcataaa atcggcggcg ctccattggc cgatgcccgt gtccgggtcg
//
LOCUS       DATASH010000179         4553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2595503,
            whole genome shotgun sequence.
ACCESSION   DATASH010000179 DATASH010000000
VERSION     DATASH010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4553
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2595503"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1207
                     /locus_tag="VHV99_05175"
     CDS             <1..1207
                     /locus_tag="VHV99_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HEX3634869.1"
                     /translation="APVNTLRHAQALPPIGAANPLRPSLDTLDSTGWLDLGSEPVIVS
                     LPDSRGRYVDARVLDMWTNVVWSTSSQFANRAAGIKAQTIAFVGPGWQGDLPKGVKRV
                     DVPTRNAWVSVRIQSNGTRDLTAVRKLQRAIRVAPLSVYAGDTRAASIAPPRSNAADA
                     AAATASGTPAAQVAALDANGFFSKLAQALPDNPPTPADPHALKILSDLGVTPGDPVKL
                     PSAPAAIAAGLADGHERIVTPPSNLLSGNGWSWFGDGVGNYGPDYAMRAYAAATQPGI
                     GTKEDEVRAVVTQDSDGHALNGANRYVIHFAPNQLPPVRGFWSITAYTKDGALGESAP
                     ARLAVGDRNGARRNRDGSLDVTVSSARGKSGNWLPAPRADLQLVLRLYAPKPQATDGS
                     WQPPAVVRQ"
     gene            1517..4553
                     /locus_tag="VHV99_05180"
                     /pseudo
     CDS             1517..4553
                     /locus_tag="VHV99_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428681.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
BASE COUNT          853 a         1498 c         1408 g          794 t
ORIGIN      
        1 ggcgccggtc aacacgctgc gccacgcgca ggccttgccg ccaatcggcg cagccaatcc
       61 gctgcggccc agtctcgaca cgctcgactc gaccggctgg ctggacctcg gcagcgagcc
      121 ggtgatcgtg tcgttgcccg actcgcgtgg ccgctacgtg gacgcccgcg tgctcgatat
      181 gtggaccaac gtggtgtggt cgaccagctc gcagtttgcc aaccgcgcag cgggtatcaa
      241 agcgcaaacg attgccttcg tcggcccggg ctggcagggc gatctgccca agggcgtcaa
      301 acgcgtcgac gtgccgaccc gtaacgcctg ggtgagtgtg cgtatccagt cgaacggtac
      361 ccgcgacctg acggccgtgc gcaagctgca acgcgcgatc cgcgtcgcgc cgttgagcgt
      421 ctatgccggc gacacgcgcg cggcgtcgat tgcgccgccg cgcagcaatg ccgccgatgc
      481 cgccgccgcg acggcttccg gcacgccggc cgcgcaagtc gccgcgctcg acgcgaacgg
      541 cttcttcagc aaactggcgc aggccttgcc tgacaatccg ccgacgccgg ctgatccgca
      601 tgcgctgaaa atcctctcgg acctgggcgt gacccctggc gatccggtga agctgccgag
      661 cgcgcccgcg gcgatcgccg cgggcctggc tgacggccac gagcgcatcg tcacgccgcc
      721 ctccaatctg ctgagcggca acggctggag ctggttcggc gacggtgtgg gcaactacgg
      781 cccggactac gcaatgcgcg cttacgccgc cgcgacccag ccgggcatcg gcacgaagga
      841 agatgaagtg cgcgcggttg tcacccagga cagcgacggt catgcgctca acggcgcgaa
      901 tcgctacgtg atccacttcg cgccgaacca gttgccgccg gtgcgcggct tctggtcgat
      961 caccgcctac acgaaggacg gcgcgttagg ggaaagcgcg cccgcgcgtc tggcggtcgg
     1021 cgatcgcaac ggtgcgcgcc gcaatcgcga cgggtcgctc gacgtcacgg tgtcgtcggc
     1081 gcgcggcaag agcggcaact ggttgccggc gccgcgcgcc gatctgcaac tggtgttgcg
     1141 tttgtacgcg ccgaagccgc aagctaccga cggcagctgg caaccgccgg cggtcgttcg
     1201 gcaatgaagt gcgcgggtga attgcgccga taaagttcgc caatgaagtt cgccaacgca
     1261 atgcgtcggc gaatgcgaca gtgaagcgca tcagcatcag tgatatgcgt cactgctgtg
     1321 acagcaatgc atgagtaaca cataactccg tcccgtgtcc gccgcttcgt gccggcggac
     1381 atgcggcgct gtaacgcgca acaatgtgta agccccagct tacatttgta cgccgcgtaa
     1441 gattcaccac ttatactgcc gcttgcggga ttttcgtttc gcggcgggct tgccgccgca
     1501 ttacccaaga ccgagcatgg caagcctcaa taaagcgtca ttcgcctctt ccatgcagac
     1561 cgtgcgcgac gtcgcgcagt cacgccgtac gcggcgtgtc atcaccggtc tgctgatctt
     1621 tctcgtattg ttcggtctgc tgggcttttt cgcggcgccg ccgttgattc gccacatcgc
     1681 cgagcaacaa ctcagcaagc aactcgaccg gcccgccacg atagggcaca ttgcgctgaa
     1741 tccgtacacg cttagattcg aagccgatcg cgtgcatatc ggcgagcgcg gcggcgcggg
     1801 tgatttcgtc gatatttcgc ggctcatcgt gaagccgtcg tggagttcgc tgtttcgcgc
     1861 cgcgccgatc atcgacgaag tgcaactcga ttcgccgcgt tttcatatcg tgcgctacga
     1921 cgcgcagcgc ttcaatttca ccgatctgat cgagaaattt tcgaagcagc cggccaagcc
     1981 cgatagcaag ccgacgctct tttcggtgtc gaacattcgc ctcgaaaatg gccagatcac
     2041 gttcgacgac aagctgctcg gcgcgaccca tgtgatcgat caatggaagc tcggtattcc
     2101 attcatcgcc acgctgcctt cgaaaaccga catcttcgtc gagccgcttt tgcgtgcgcg
     2161 ctttgatggt agtccgctcg ctatcgacgg caagaccaag ccgtttgcag cgtcgcgcga
     2221 gtcggaggtg tcgctgcgtt tcgacgggct cgacgtgccg cgtctggtgt cctatgtgcc
     2281 gaccaagctg cctgtgatcg tgcagagcgg caagctgtcc accgatctca agctcaactt
     2341 cgtggtctcc aatgacgcac cttcgttgcg cgtggcgggc acggtcgatt tgaacgatgt
     2401 ggacctgcgc gaccaaggca aggcgccgtt cttcgcggcg cgcgcagtgc atgtggcggc
     2461 cgcgacgctc gagccgttga agagcctgta tcacttcgac gatattcgca tcgacgcgcc
     2521 aagcgccaat cttgcgcgtg acaaagacgg cgtgttgagt gtcgagaaga tgttcgcgcc
     2581 tgcgcctgct gcgggggctt ccacgtcagc atcggcgtct gcttccgcgc cggcatccgc
     2641 ggcggccgct tctgcggcca cgacggctaa aaacgccgcc agcaacgccg ccagcgcacc
     2701 cgtcgcggca tccggtgcag ccgccgcgga taagaccgca cagcctctgg acctgtcgat
     2761 caagcgtttc gcgctcaacg acggcaccgt aaacgttcat gatgggccgc ctcgcggccg
     2821 gtcgaccttg gcctgcaaaa actggccgtc acgctgaccg atttctcgac gctcgccact
     2881 acgcctgcgc actacacgct gaactcagac ttcaaggatg cgggcggttc gctcggtgcg
     2941 gcgggtgcgt tcggcctcgt cgcgaagacg gccagtgcga agctcgatct gaagtcgttg
     3001 aagttgccgc tgctgcaacc gtatctcgac accgcaacgg ccgcgcaggt ggtggacggc
     3061 gcgctgtccg ccaacgcgaa catcggcgcg aactggtcga agtccccggc cgccgtgatg
     3121 gtcaccgaca cgcaactcga tctgcagtcg ctcaaactcg cagcacgcgg cagcaaggag
     3181 ccagtcgtct cgctcgcgca aggcacggtg acggtcaagc aggtagacgt cgcgggacgc
     3241 actgccgata tcaccggcgt cgacacgacc ggtctggtgg tcaacgcgca gcgtctgaaa
     3301 gacggcagta tcgacctcgc cgcgctcggc ggtccgcacg agggcgcgca gcagcgcacg
     3361 gcgatccatg cggtgaagaa ggcgcaagcc gaagggcccg cgtggcacta caagatcggc
     3421 gaactcaacc tgaaagacgc gagtgccaac ttcaccgaca acaccacgcc gcggccggtg
     3481 aagctgagcg tgacgccgct gcaactgaaa gtgcagcaga tcagcgacga tctcagcaag
     3541 ccgctgccga tcgatctgca agccacgctc aaccagaaag gcacgctcgg cgttaaaggc
     3601 gatgtcacag ccacaccgct caaggtggcc gtcaaggtga acgcgaaccg tctggatgcg
     3661 gcggccttcg aaccgtactt cggcagcaag ctgaatgcgt tgatcgcgag cgcattgctc
     3721 aatgcgagcg gcgatctcgc gctgagtcag gcgaaagcgc tcaaggcgac gtaccgcggc
     3781 gacgtggcgc tcgtcgacgt acggatgctc gacaaggtga cgtctgatcc attcgcgggt
     3841 tggggttcgc tcgcgctgac caatctgaca gccgactacg acgaacacgg cacgaccgtc
     3901 gacgcgggcc gcgtgacgtt caccaggttc tatggacgtg tgctgctcga cgcgcaaggc
     3961 aagctgaatc tgaaggacgt ggtcgcgcat gaaagcggcg ccgcgcaatc gctgacacgc
     4021 gacaagagcg gccacgatcc ggtgccgttg acgcccgagg ctgcttctgc gcccgtggtg
     4081 gcgtccgctc cggttcccgc atcttctgcg acgccggcca cggtgagcgc cgctacgccg
     4141 ccgcaaagcc ccgtcaaact gcacttcggc caactggtgc tgcaacaggg ccgcgtgacg
     4201 tacaccgaca acttcatcaa gccgaacttc acggcgaacc tcgtcaatat tcagggcacg
     4261 gtcggcgcgt tcggcacgca atctaccacc tccgcgcctg tcgacattgc cgcgaagctg
     4321 gccgccaatg gtccgctatc gattcgcggc acggtcaatc cgctgatcgc gaaaccggcg
     4381 ctcgatttga ctgccagcgc gcatgatatc gagctcacca atctcacgcc atattcggcg
     4441 aaatacgccg ggtatccgat taccaaaggc aagctgaatg tcgatctgca ctacaagctc
     4501 gataacgatc agttggacgc gaacaatcat attttcatcg accagcttac gtt
//
LOCUS       DATASH010000180         3224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4969321,
            whole genome shotgun sequence.
ACCESSION   DATASH010000180 DATASH010000000
VERSION     DATASH010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4969321"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(242..787)
                     /locus_tag="VHV99_05185"
     CDS             complement(242..787)
                     /locus_tag="VHV99_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018418857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HEX3634870.1"
                     /translation="MAVKTSNQAPKNGRDDHRAERTISDASTGQHVATDDAWDVEQQD
                     NNIVPLTRAEAEKLFGPNVSRPSRVTPFKVVAAQMVLSLGATLVWWLFYKPPGAAALS
                     AFLGGAICWVPGALFAARLRTLSGAETVMSWMIGEALKMGTTIAMFVAIAFWYHDVRW
                     VPLLVTYLIALKTYWIALAWR"
     gene            complement(1710..2600)
                     /locus_tag="VHV99_05190"
     CDS             complement(1710..2600)
                     /locus_tag="VHV99_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007581688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX3634871.1"
                     /translation="MNAVARKKGLGRGLEALLGGSADITEAAVMQGAPNVLPLSKLQA
                     GKYQPRTRMDEGALQELAASIRAQGLMQPILVRPVSADKYEIIAGERRFRAARLAGLD
                     EVPVLVKDVPDQAAAAMALIENIQREDLNPLEEAQGIQRLLDEFNFTHEQAAESVGRS
                     RSAVSNLLRLLNLAAPVQTMLLAGDLDMGHARALLAVDAATQITLANQVVNKRMSVRE
                     TEKLVTATTKVAPAVKARANQDGGRDTRRLEEELSDLLAATVKIKLGRRGRGQVLVDF
                     GDLDALEGILVRLRGNATVA"
     gene            complement(2619..>3224)
                     /locus_tag="VHV99_05195"
     CDS             complement(2619..>3224)
                     /locus_tag="VHV99_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX3634872.1"
                     /translation="VLVDGVSVADARVRPEAVGYDMLPANRELAGAEVELVSVQNRER
                     QLKAALAAVESEYDFILIDCPPALSLLTLNGLCAAHGVVIPMQCEYFALEGLSDLVNT
                     IKQVHANLNRDLKVIGLLRVMFDPRITLQQQVSDQLKEHFGDKVFDVVIPRNVRLAEA
                     PSYGLPGVVFDKASRGAQAYVQFGAEMIERVRTLNDAPLAS"
BASE COUNT          667 a         1037 c          891 g          629 t
ORIGIN      
        1 tgcgcggtgg aaaagttctg caagtggtgc gcgatgtact cggacggatc cagagcgcgc
       61 gtgccttcgc tagctgccat atcgttaatg ccacccaaat tgtcgaaaat cgtcttaggc
      121 cgctcccgcc acaatgctgt attgcgggaa cacgccgggt gcggatcgtg tcatatccgc
      181 actccttatg ccagcgccgc gcgccagcct gaattttgtg ttgctattct ctgctactac
      241 cttagcgcca cgcgagggcg atccagtaag ttttgagcgc gatgaggtag gtcacgagca
      301 gcggcaccca gcgtacgtcg tgataccaga aagcgatggc tacaaacatc gcgatcgtcg
      361 tccccatctt gagcgcttcg ccgatcatcc agctcatcac tgtttcggcg ccactcagcg
      421 ttctaagacg tgccgcgaac aacgcgcccg gcacccagca gatcgcccca cccaggaacg
      481 cggacagcgc agcagcgccc ggcggcttgt agaacagcca ccacaccagc gtagcaccca
      541 gggacaaaac catttgcgcc gccacgacct tgaaaggcgt gacacgcgat ggacgactca
      601 cattcggacc aaacagcttt tcagcctcag cccgcgtgag cggaacgata ttgttatctt
      661 gttgctcgac atcccacgca tcgtcagttg caacgtgctg accggtggac gcatcggaaa
      721 tcgtgcgttc agcgcggtgg tcgtcacgcc cattcttcgg cgcttgattc gacgttttaa
      781 ccgccatcgc agtgttccga aagtcttgtt ccagcccgag agcttacgct gcgcttacgg
      841 ggttgccgtg caattaaatc cgggcgattg taagcgatag ttgcaagcaa ttcaagactt
      901 taggccgttc aataaccagc atgaaaacgg ccttcacgat gcgggaatgg gattttgggc
      961 gccctgtaca cggcaaatgt aaggcggccg gggaacggca aaacatgcgc aatatatcgg
     1021 gtcggctgaa gaatgtggat gcgtcgtttc gacgctgtgc ggacgtctca aaaatgcaac
     1081 agccgatggt ttttgccagc tttggtggac atggggcgcg gcaccgggta gagggcgaga
     1141 tggtgaatat ggctgcgacc gttccgctgc gaccagacag cgtggaacgc cgccctcaga
     1201 accgaacgat cgacgcagcg gggaccgggc gccgatcgct tcatctcgaa gcctcccgcc
     1261 ctaccgcgct aacggctccg ctcacatgtc gcacacgtct tctaccgcca gatcctaatg
     1321 ggcgaaaggc tgcgtatccg gaactcgtcg aggcagcgca gcggtcaagc tcagccccac
     1381 cgactgccac atccgagggt ccatcaaatc gccgcgcaac tcggctatgt ccgcctgcat
     1441 cgacgcaaaa tatcagcgca caacgatgca tcgcattcct gattacttag cttagtgaaa
     1501 cccatggtta ggaatccacg cgatattagg tcggtcttcg aataatcaga gaggtcgcaa
     1561 gaggaaacct gcttaagggg gctacgaccg ttgcacgatc gccatcgctg cagcaaacac
     1621 acgagacacg ccaaacagct tacgagcggg ttcgaaaccc agccaacccg caagcacgtt
     1681 caacatccct cacgttcaac ctgccaggat caggcgaccg tggcgttgcc tcgcaaccgg
     1741 acaagaatgc cttccaatgc gtccagatcg ccgaagtcca ccagcacctg cccgcggccg
     1801 cgccggccga gcttgatctt taccgttgcg gcaagcagat cggataactc ctcttcgagc
     1861 cgtcgcgtgt cgcggccgcc gtcctgattc gcgcgcgcct tcactgcggg cgcaaccttg
     1921 gtggttgccg tcaccagttt ttccgtctcg cgtaccgaca tgcgcttgtt gacgacctgg
     1981 ttggccagcg tgatctgcgt tgcggcgtcg acggccagca gcgcgcgtgc atgccccatg
     2041 tcgagatcac cggccagcag catcgtttgc accggcgccg cgaggttgag caggcgcaac
     2101 agattcgata ccgcactgcg cgagcggccc accgattcag ccgcctgctc gtgtgtgaaa
     2161 ttgaactcgt cgagtaagcg ctgaatgccc tgcgcttctt ccagcggatt caaatcttcg
     2221 cgctgaatat tctcgatcag cgccatggcc gcggcggcct ggtccggcac gtcttttacc
     2281 aacaccggca cctcgtcgag gccggcaagg cgtgcagcgc ggaaacgccg ctcgccggcg
     2341 ataatctcat acttatccgc cgaaaccggt cgcaccagaa tcggctgcat tagcccctgg
     2401 gcacgaatgc tggccgccaa ttcctgtaac gcaccttcgt ccatgcgcgt gcgaggctgg
     2461 tacttgccag cctgcagttt gctgagcggc agtacgttcg gcgccccctg catcactgcg
     2521 gcctcggtaa tatccgcgct gccgccaagc aatgcttcca gaccgcggcc caagcccttc
     2581 tttcttgcta ctgcattcat tgtgcttcct cgatccgctt aggacgccaa gggcgcgtcg
     2641 ttcaacgtgc gcacccgctc aatcatctct gcaccgaact gcacgtacgc ttgcgcgccg
     2701 cgcgacgcct tatcgaacac aacacccggc aacccatagc taggcgcctc ggcaagacgc
     2761 acgttgcgtg ggatcaccac gtcgaacacc ttgtcgccga aatgctcttt cagttgatcc
     2821 gagacttgct gttgcaaggt gatgcgcgga tcgaacatga cgcgcagcag gccgatcacc
     2881 ttcagatcgc gattcaggtt cgcatgcacc tgcttgatcg tgttcaccag gtcggacaga
     2941 ccctcgagcg cgaagtactc gcactgcatc ggaatcacca cgccgtgcgc ggcgcacaag
     3001 ccgttgagtg tcagcagcga caaagccggt gggcagtcga tcaatatgaa gtcgtattcg
     3061 ctttcgaccg ccgccaaggc ggccttcaat tggcgctcac ggttctgcac actgaccagc
     3121 tcaacctcgg cgccagccag ttcacggttg gcgggcagca tatcgtagcc gacggcttcc
     3181 gggcgtactc gcgcatcggc caccgatacg ccatccacca acac
//
LOCUS       DATASH010000181         6683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4376028,
            whole genome shotgun sequence.
ACCESSION   DATASH010000181 DATASH010000000
VERSION     DATASH010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6683
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4376028"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1692)
                     /locus_tag="VHV99_05200"
     CDS             complement(<1..1692)
                     /locus_tag="VHV99_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX3634873.1"
                     /translation="MTNPTSALLVKPIHLVPSAARLTTPLAQHLRKSLRGDVLFDAAS
                     RGRYATDASIYQITPIGVVVPRDQDDLRIALEIARSEKVPLLARGAGTSQCGQTVGEA
                     LVIDTSKWLNNIVAFDAEARTVTVEPGVVLDHLNAWLKPHGLWFPVDVSTAAQCTIGG
                     MAGNNSCGSRSIEYGNMVHNVEAIDAILADGSEARFASLREAPQGARLQQIFEGVKRI
                     AEREHDEIVERIPKVLRRVAGYNIDVFDCQNPRAYTDDGVANLAHLLVGSEGTLAFSR
                     QLTLKLAPLPAHKTLGVVNFPTFWQSMDLAQHIVKLKPVAVELVDRTMIDLAMSNPAF
                     RPVIEKALVGEPQAILLVEFAGENRDEQLASLKQLTELMADLGLPDSVVEMHAANEQK
                     ALWEVRKAGLNIMMSMKGDGKPVSFIEDCAVPLEHLAEYTSRLTEVFHRNGTEGTWYA
                     HASVGTLHVRPILDMRRDGAMKMRAIAEEAAALVREYKGAYSGEHGDGLCRGEWVAWQ
                     YGPRLNQAFSEIKALFDPDNRFNPDKIVRPPKMDDARNFRFAPGYEVHRIETALDW"
     gene            complement(1717..2466)
                     /locus_tag="VHV99_05205"
     CDS             complement(1717..2466)
                     /locus_tag="VHV99_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018438430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HEX3634874.1"
                     /translation="MTTLLSQRLADARRQHLTLAALPDEQTPADGAAAYAIQHDLLRI
                     LDASIGGWKIGAKSDSGPIQGAPLPASDLHADGARLPREAFTPLGLELEIAFRFGRRF
                     EPSTTPYSEAEVRAGIGSFGATIEIVASRYAGWPNVDKFAQLADLQNHGALIVGEFAP
                     YREDFPCAAPQLRFCFEGNDVVKETPANPAGDPRRLLTWLVNHATSRGIAVTPEMVVT
                     TGSYTGIFFPQGAGTASGHIEGLAPISVTLY"
     gene            complement(2504..3790)
                     /locus_tag="VHV99_05210"
     CDS             complement(2504..3790)
                     /locus_tag="VHV99_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3634875.1"
                     /translation="MKRFRVSSATSIVLVMLSIMYFITYLDRVNVSTAAAGFGKEFGL
                     SHTEVGLVFSAFAYPYLIFQIIGGWVSDRFGAKRTLIFCGAIWGVATLLTGLAGGLAS
                     LLAARVLLGFGEGATFPAATSAMARWVAKEKRGFAQGITHAASRIGNAVAPAFIVLVM
                     ATWGWRESFYICGVFSLLWVAVWSFTFTEHPKDHPRITSEELEILPAPKPKVAAVPWG
                     KLFRRMWPVTIVYFCYGWTLWLFLSWIPQYFLHSYHLQLQKSAIFASVVFFAGVLGDT
                     LGGIATDWIFTRTRSLKRARSWMVSVCMLFCLLSLIPLMFTHSLYLSMACLASGFFFA
                     EMTIGPMWAIPMDIAPEYSGTASGMMNTGSALAAIISPVAGGFLIDYFGSWELPFAGS
                     MLLMAIGVVLALRMQPESKFEFNAADKAHATTGAGA"
     gene            complement(4129..5349)
                     /locus_tag="VHV99_05215"
     CDS             complement(4129..5349)
                     /locus_tag="VHV99_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007744696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX3634876.1"
                     /translation="MLKLDFHPAGRHFLQITGPSPVPDRILRAMSYPTIDNRGPEFGE
                     LGLKVLEGIKKIFKTQQPVVIYPASGTGAWEAALSNTLSPGDNVLMFETGHFATLWKK
                     MAESLGLKPEFLGLPGIEGWRRGVQPQMIEERLRADTQHAIKAVCVVHNETSTGVTSD
                     IAAVRRAIDAAGHPALLLVDTISGLACADYRHDDWGVDVTVSGSQKGLMLPPGISFNA
                     ISPKAFAASKQAKLPRSFWDWTDIVEMNKTGYWPYTPNTNLLYGLNEALEMILGEGLD
                     NVFARHARLAEATRRAVRAWGLEIQCADPAVYSPVLTGVMMPEGIDADAVRKVIYERF
                     DMSLGTGLGKMKGRMFRIGHLGDCNDLMLLATLAGCEMGLRIAGVPLKESGLPAAMEW
                     LSQPTQASGLKAAA"
     gene            5510..6235
                     /locus_tag="VHV99_05220"
     CDS             5510..6235
                     /locus_tag="VHV99_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX3634877.1"
                     /translation="MQNSDFAAVVTASPLMPKVERQRLHDTVVEHIRRFIVEGVLEPG
                     KKLNERELCETLGISRTPLREALKVLAAEGLIEISPNRGASVSKMSEAELRETFELMS
                     GLEAFSGELAAERMTAAELAEIKALHYAMLACRTQNDLAGYYSRNQAIHDKINEAARN
                     SALRQTYVAVNRRLQALRFRSNFQVPKWDSAIHDHDEMLKALEARDGKKLSAILRQHL
                     LDKRDAVLQVQSREEVAASPLKA"
BASE COUNT         1260 a         2025 c         2044 g         1354 t
ORIGIN      
        1 ccagtcgagc gcggtctcga tccgatgcac ttcgtagccc ggcgcaaagc ggaaattgcg
       61 cgcgtcgtcc atcttcggcg gacgcacgat cttgtcgggg ttgaagcgat tatccggatc
      121 gaacagcgcc ttgatctcgc tgaacgcctg gttcagacgc ggcccgtatt gccatgcgac
      181 ccattcgccg cgacacaaac cgtcgccgtg ttcgcccgaa taggcgccct tgtattcgcg
      241 gaccaatgca gcggcttcct cggcgatcgc gcgcatcttc atcgcgccgt cgcgccgcat
      301 atcgagaatc ggccgcacat gcagtgtgcc gacgcttgca tgcgcatacc aggtgccttc
      361 tgtgccgttg cggtggaaga cttcggtcaa acggcttgtg tattccgcca gatgctcgag
      421 cggcaccgcg cagtcttcga tgaaggagac tggcttgccg tcgcccttca tgctcatcat
      481 gatgttcaag ccggctttgc ggacttccca caacgctttc tgctcattcg cggcatgcat
      541 ttcgacgacc gaatcgggca agcccaaatc cgccatcagt tcggtgagtt gcttgagcga
      601 cgcaagttgc tcatcgcgat tctcgcccgc gaactccacc agcaggatcg cttgcggctc
      661 gccgaccagc gccttctcga tcaccggccg gaaggcgggg ttgctcatcg ccagatcgat
      721 catcgtgcga tcgaccagtt caacggccac cggtttgagc ttgacgatgt gttgcgcgag
      781 atccatcgac tgccagaagg tcgggaaatt caccacgccc agcgtcttat gcgcgggcag
      841 cggcgcgagc ttcagcgtca gttgccggct gaacgcaagc gtgccctccg aaccgaccag
      901 cagatgcgcg aggttggcga cgccgtcgtc ggtataggcg cgcgggttct gacaatcgaa
      961 cacgtcgatg ttgtagcccg ctacgcggcg tagcaccttc ggtatgcgct cgacgatttc
     1021 atcgtgctcc cgctcggcga tccgcttcac gccttcgaat atttgctgca aacgcgcgcc
     1081 ttgcggcgcc tcgcgcaatg aggcgaaacg cgcttcgctg ccgtcggcga gaatcgcgtc
     1141 aatggcttcg acgttgtgca ccatgttgcc gtattcgatc gagcgcgagc cgcaggagtt
     1201 gttgccggcc atgccgccaa tcgtgcattg cgctgccgtc gatacatcga ccggaaacca
     1261 caaaccgtga ggcttcaacc atgcgttcag atggtccagc acgacgcccg gttcgaccgt
     1321 cacggtgcgc gcttcggcat cgaatgcgac gatgttgttc agccatttgc tggtgtcgat
     1381 caccagcgct tcgccgaccg tctggccgca ttggctcgtg cccgcgccgc gtgcgagcaa
     1441 cggtactttc tcgctgcgtg cgatctccag tgcaatgcgc agatcgtcct gatcgcgcgg
     1501 caccaccacg ccgatcggcg tgatctgata gatcgacgca tcggtcgcgt aacggccgcg
     1561 gctcgcggca tcgaacaaca cgtcgccacg caacgatttg cgcaaatgct gcgcgagcgg
     1621 cgtcgtcagg cgtgcagccg acggcaccag atggatcggc ttgacgagta aagcggaagt
     1681 ggggttcgtc atatcgtcgg cctataggaa tgcgggctag tacagcgtca cgctgatcgg
     1741 cgcgagccct tcaatgtgcc cgctcgccgt acccgcgccc tgcggaaaaa agatcccggt
     1801 gtacgaaccg gtggtgacga ccatctccgg cgtgaccgcg ataccgcgcg aggtggcgtg
     1861 attgacgagc catgtcagca ggcgtcgcgg atcgccggcc gggttggcgg gcgtctcctt
     1921 gaccacgtcg tttccctcga agcagaaacg cagttgcgga gccgcacaag gaaagtcctc
     1981 gcgatacggc gcaaattcgc caacgatcag cgcgccgtga ttctgcaaat ccgcgagctg
     2041 ggcaaacttg tcgacgttgg gccagccggc gtaacggctc gccacgatct cgatggtcgc
     2101 gccgaacgag ccaatgcccg cgcgcacttc ggcttcgcta tacggtgttg tggagggctc
     2161 gaagcggcgg ccaaagcgga atgcgatctc cagttcgagc cccaacggcg taaaggcttc
     2221 gcgcggcaac cgtgcaccgt ccgcatgtaa atcactcgcg ggtaacggcg cgccctggat
     2281 cggcccgcta tccgacttcg cgccgatctt ccagccgccg atcgacgcgt ccagtatgcg
     2341 caggaggtcg tgctggatcg cgtaggcggc ggcgccgtca gcgggcgttt gctcgtccgg
     2401 caacgcagcg agcgtcaggt gctggcggcg tgcatcggcg agacgttgac tcaatagtgt
     2461 cgtcatttct ttagaggtga agagagggaa caacgcattc cgtttatgcg cccgcacccg
     2521 tcgtagcgtg tgctttgtcg gccgcattaa actcgaactt gctttccggc tgcatgcgaa
     2581 gcgcgagcac cacgccgatc gccatcaaca gcatgctgcc ggcgaacggc aactcccagc
     2641 tgccgaagta gtcgatcagg aatccgccgg ctaccggcga gatgatcgcg gccaatgccg
     2701 agccggtgtt catcatgccg ctcgcggtgc ccgagtattc cggcgcgatg tccatcggga
     2761 tcgcccacat cgggccgatc gtcatttcgg cgaagaagaa acccgacgcg agacacgcca
     2821 tcgacagata caggctgtgc gtgaacatca gcggaatgag cgagagcaga cagaacagca
     2881 tgcacaccga caccatccag ctacgagcac gtttcaggct gcgggtgcgc gtgaagatcc
     2941 aatcggtcgc gatgccgccg agcgtgtcgc caagtacgcc ggcaaagaac acgaccgacg
     3001 cgaagatcgc cgatttttgc agttgcaggt gatagctatg caggaagtac tgcggaatcc
     3061 agctaagaaa cagccacaaa gtccagccgt agcagaagta cacgatcgtg accggccaca
     3121 tgcggcgaaa caatttgccc cacggcacgg cggccacttt cggcttcggt gcgggcagaa
     3181 tttcgagttc ttcgctcgtg atacgcggat gatctttcgg atgctcggtg aacgtgaacg
     3241 accacaccgc cacccacagc aggctgaaca cgccgcagat gtagaacgat tcgcgccagc
     3301 cccaggtcgc catcaccagc acgataaaag ccggtgccac cgcattgccg atacgcgatg
     3361 ctgcatgcgt gatgccctgc gcgaaacccc gtttttcttt cgcgacccag cgcgccatgg
     3421 ctgaagtggc cgcgggaaac gtggcgcctt cgccgaaacc aagcagcacg cgcgcggcca
     3481 gcaacgacgc caggccgccg gcaagaccgg tcagcaaagt cgcgacgccc cagatggcgc
     3541 cacagaaaat cagcgtgcgt ttcgcaccga aacgatcgct gacccagcca ccgatgatct
     3601 ggaaaatcag atacggatag gcgaaggccg aaaacaccag gcccacttcc gtatgcgaga
     3661 gcccgaactc cttgccgaat ccggcggccg cggtactgac attgacgcgg tccaggtagg
     3721 tgatgaagta catgatgctt agcatcacta gaacgatact agtcgcactg cttacacgaa
     3781 accgcttcat cgtctctctc cgttgcagtc tatcgacggc gcgcctggaa tggcgtgctg
     3841 cgtcgtcgcc cgtcagcgac gtgcgctgcg ggacccttgc atggtgttcg tgggccgcgt
     3901 tctatgcgcg gcttcatgag ggaattaccc ccgtttttcc ttggctacga gatgagctgt
     3961 tgcctgctgc ctgttgccta ttgtctggtg ctactgctgc ctgctgctgg ttgtgtggac
     4021 gcgctgacgc accgatacgc gtggtcccgt tccgcgactg catcgaagtg cgccatagcg
     4081 ccggcggttg cagttgcagt tgcgctaacg caccacgcgc ccaagtgttt aagccgctgc
     4141 tttcagcccc gaggcttgcg tcggctgact cagccactcc atagcggcag gcaagccgct
     4201 ttccttgagc ggcacacccg cgatccgcag acccatttcg cagccggcga gcgtcgccag
     4261 cagcatcagg tcgttacaat cgccgaggtg gccgatgcgg aacatgcgtc ctttcatctt
     4321 gccgaggccg gtgccgagcg acatgtcgaa gcgttcgtag atgaccttgc gcacggcgtc
     4381 ggcgtcgatg ccctcgggca tcatcacgcc ggtcagcacc gggctataca cagccggatc
     4441 cgcgcactgg atctccaggc cccaggcacg caccgcgcgg cgggtggctt cggcgagacg
     4501 cgcgtggcgg gcgaacacgt tgtctagccc ttcgccgaga atcatctcca gcgcctcgtt
     4561 gagcccgtac agcaggttcg tgttcggcgt gtacggccag tagccggtct tgttcatttc
     4621 gacgatgtcg gtccagtccc agaaactgcg cggcagtttg gcctgtttgc tggcggcgaa
     4681 cgccttcggc gaaatggcgt tgaagctgat gcccggcggc agcatcaaac ctttttgcga
     4741 gcctgacacg gtgacgtcca cgccccaatc gtcgtggcga taatcggcgc aggcgaggcc
     4801 cgagatcgtg tcgaccagca gcaacgccgg atgacccgcg gcgtcgatcg cgcggcgcac
     4861 ggcggcaata tccgacgtga cacccgtcga ggtttcgtta tggacgacgc acaccgcctt
     4921 gatcgcgtgc tgcgtgtcgg cgcgcagacg ttcctcgatc atttgcggct gaaccccacg
     4981 ccgccagccc tcgatacccg gcaggccgag gaactccggc ttcagcccca ggctttccgc
     5041 catctttttc cacagcgtcg cgaagtgccc ggtttcgaac atcagcacgt tgtcgccagg
     5101 gctcagcgta ttcgacagcg ccgcttccca agcgccggtg ccggaggccg gatagatcac
     5161 caccggctgc tgcgtcttga agattttctt gatgccttca agcaccttca gaccgagttc
     5221 gccgaattcg gggccgcggt tatcgatggt cgggtagctc atcgcacgga ggatgcgatc
     5281 gggcaccggg ctcggacccg tgatctgcag gaagtgacgg ccagcggggt gaaagtctaa
     5341 cttgagcatt gggtttctcc gattgaattt tgcatgcaaa aaactatatc agacgatgtg
     5401 cgataatcca aaggtcaaaa ttgcctgctg gcaccggtgt ttacccgtgc tgttaactaa
     5461 tctgtatgct tccgataaaa tcccgcttac atgggttgag ggcttttgta tgcaaaattc
     5521 ggatttcgcc gcggttgtca ccgcttcccc tctgatgccg aaggttgagc gtcagcgctt
     5581 gcacgacacg gtggtggagc acattcgccg ctttatcgtc gagggggtgc tcgagccggg
     5641 caagaagctg aacgagcgcg agttatgcga gacgctcggg atttcgcgca cgccgttgcg
     5701 ggaggcgttg aaggtgctgg ctgcggaagg gctgatcgag atttcgccga atcggggcgc
     5761 gtcggtgtcg aagatgtcgg aagcggagtt gcgcgagacg ttcgaattga tgagtggtct
     5821 tgaagctttt tccggcgagt tggcggcgga gcggatgacg gccgccgagc ttgccgagat
     5881 caaggcattg cattacgcga tgctggcttg ccggacgcag aacgatctgg cggggtatta
     5941 cagccgtaac caggcgattc acgacaagat caatgaagca gcgaggaatt cggcgctgcg
     6001 gcagacttat gtcgcggtta atcgacgcct gcaggcgtta cggtttcggt cgaattttca
     6061 ggtccctaag tgggatagtg cgattcatga tcatgatgag atgttgaagg cgctcgaggc
     6121 gcgggatggg aagaagttga gtgcgattct tcggcagcat ttgctggata aacgggatgc
     6181 ggttttgcag gtgcagtcga gggaagaagt ggcggcttcg ccgctgaagg cttaaaggtt
     6241 ttttttgcgt tcgcggggct tgttatctgt atgcctgcgg cgttgggctt tccttgaatt
     6301 gctggcctac tcggcgctcg ctatctgtat gcctatggcg ttggcctttc cttgaattgc
     6361 tggcctactc ggcggttgct atctgtatgc ctgtggcgtt ggcctttcct cgaattgctg
     6421 gcctactcgg cgctcgctat ctgtatgcct acggcgttgg cctttccttg ttttcttagt
     6481 ggtttattag cgtcgcccct gtgcggggca ggcacctact tttctttgcc tgctgcaaag
     6541 aaaagtaggc aaaagaaagc agctcaaacc gccagctcat aagcgggtcc cccgcacagc
     6601 tacggtagtg gtgcatctgg aatcggtgtt ctcgcacact ccgcgtcagt gacacagcag
     6661 tcatacttcc ggcggcgctc cgc
//
LOCUS       DATASH010000182         9574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2398456,
            whole genome shotgun sequence.
ACCESSION   DATASH010000182 DATASH010000000
VERSION     DATASH010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 9574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9574
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2398456"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /gene="rsmH"
                     /locus_tag="VHV99_05225"
     CDS             complement(<1..495)
                     /gene="rsmH"
                     /locus_tag="VHV99_05225"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012402052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX3634878.1"
                     /translation="MAPAMGNELQHRTVLLEEAVNALVTRADGVYVDGTFGRGGHSRL
                     VLEKLGEAGRLIAFDKDPLAIATAQQIADPRFGIVHESFASLRDAIAERGVGRVSGVL
                     LDLGVSSPQVDDPERGFSFRADGPLDMRMDPTRGESAADWLARATVQELTEVIRDYGE
                     ERFAF"
     gene            complement(511..939)
                     /gene="mraZ"
                     /locus_tag="VHV99_05230"
     CDS             complement(511..939)
                     /gene="mraZ"
                     /locus_tag="VHV99_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019255554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX3634879.1"
                     /translation="MFQGASALTLDAKGRMSIPSRYRDALQTQAEGRVTITKHPDGCL
                     LLFPRPEWEIFRDKVDKLPMNAAWWKRIFLGNAMDVDMDGAGRVLVSPELRAAGSLEK
                     EVTLLGMGRHFELWDAQIYAAKEQAAIAEGMPEALKNFTF"
     gene            complement(1460..2092)
                     /gene="coq7"
                     /locus_tag="VHV99_05235"
     CDS             complement(1460..2092)
                     /gene="coq7"
                     /locus_tag="VHV99_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HEX3634880.1"
                     /translation="MFLDELISEFDRGLRSMTGVSRMSRPLPVPQGLAAESVELSPAE
                     RAHAAGLMRVNHVGEVCAQALYQAQKLATKSPSLRAVFNRAAIEEEDHLAWTAKRLEA
                     LDSRPSLLNPLWYTGALAIGLAAGRLGDRVSLGFMAETERQVEQHLDSHLDELPAADR
                     ESRAIVEQMRVDEAQHGKSALDAGGVELPFPARALMRAMSKVMTRTAYYI"
     gene            2394..3566
                     /locus_tag="VHV99_05240"
     CDS             2394..3566
                     /locus_tag="VHV99_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX3634881.1"
                     /translation="MKKSLLALAALGAFAGVAHAQSSVTLYGIIDEGFNINTNSGGKH
                     LYNLSSGVLQGSRWGLRGTEDLGGGLKAIFVLENGFDVNNGKLGQGGLMFGRQAYVGL
                     SSQFGTVTMGRQYDSVVDYVGPLEAGDQWGGYIAAHPGDIDNFNNAYRTNNTVKYTSA
                     NYGGLTFGGTYSFGGQAGNFTGNQIWSLGAGYNNGPLVLGVGYLNARTPAASGGLFNN
                     GGTALAANAAVTSPVYAGFSSANTYQVIGAGGAYTFGAATVGLTYSNIHFGALGASFA
                     SPLHTGQSATFNNAELNFKYQLTPALLVGAAYDYTRGADISGASRAQYHQGAVGVDYF
                     LSKRTDVYVTGVYQHALGDTLNAAGATVDATAGINGLTGSSNQNQFTARVGIRHKF"
     gene            complement(3789..5462)
                     /locus_tag="VHV99_05245"
     CDS             complement(3789..5462)
                     /locus_tag="VHV99_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX3634882.1"
                     /translation="MDKIWLKSYPPGVPAEIDPTRYSSVAELLEEAFRDHRAKPAFVC
                     MGKEISYGELDALSHKLAAWFQSKGLARGARIAIMMPNVLQYPVAIAAILRAGYVVVN
                     VNPLYTPRELEHQLKDSGAEAIILLENFAVTLQAIVRNTSVKHVVVAAMGDLMGVKGT
                     LVNFVVRRVKKMVPACSLPGHVKFNAAIAEGARQNFRPVQQGPDDVAFLQYTGGTTGV
                     AKGATLLHRNLIANVLQSEIWLDPVRANRTDIDQFITVVALPLYHVFALTVCGLLTIR
                     TGGLGVLIPNPRDIPGMIKALEGYPITTIPAVNTLYNAMLNSPDFHKLDFSKLIAANG
                     GGMAVQEAVAKRWFEHTHTPIIEGYGLSETSPCVTCNPVTVTEYSGTIGLPLPSTEIS
                     IRDDEGNEMPLGQPGEICIRGPQVMAGYWNRPDETAKVMTPDGFFKSGDVGLMNEHGF
                     VKIVDRKKDMILVSGFNVYPNEIEDVVARLPGVFEVAAVGVPDQHSGEAVKLFVVKKD
                     QALTDADIFAYCKQQLTGYKRPRIVEFRTELPKSNVGKILRRELRDGRA"
     gene            complement(5610..7688)
                     /locus_tag="VHV99_05250"
     CDS             complement(5610..7688)
                     /locus_tag="VHV99_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009771042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HEX3634883.1"
                     /translation="MNAPTEFARAVCPHDCPDTCAMRVTIEGGLAVKVVGDPDHPPTQ
                     GALCTKVSRYADRVHHPHRLTTPMKRIGRKGEGRFEPVSWDQAFELAAVRLAEIASRA
                     PEAIMPYSYAGTMGLVQGDSIAQRFFHKLGASQLDRTICAAAGAAGLKYTYGASLGMR
                     TEFFAESEVVLIWGSNPIASNLHFWTRAQEAKRRGARLIAIDPYRSLTAEKCHQHIAL
                     KPGTDGALALGMINVLITENLLDHAYIAAHTLGFEQLKVRALSYPPSRVAQICGVDEQ
                     VIVDVARLYGSTKKAAIRMNYGLQRVRGGGNAVRAIACLPSLTGAWRERAGGVLLSSG
                     GWAPVDSHALQRPDLMPGWPAKASRVINMNAIGDALLHPGDATFGPKVEAIIVYNSNP
                     VAVAPDSERVAAGFARDDLFTIVLEHFRTDTADYADLLLPATTQLEHLDVHKSYGHTH
                     VMVNLPAIAPVGDARPNTEIFRGLARHMGLQEPALYDSDDAIAQVAFRWNDKTLEGVS
                     WDSLKKTGWAELKLPDAPFAEGGFRTPSGKCEFFSERLAQQGLDPLPDYLPPYESADG
                     APELAARYPLAMISPPARNFLNSTFVNIESLRSTEGEPHLDIHPVDALQRNIGDGDQV
                     RIFNDRGSMQARARVTDRARKGLVVGLSIWWKKLAPDGRNANQVTSQALTDLGGSATF
                     YDCLVEVERA"
     gene            7865..9061
                     /locus_tag="VHV99_05255"
     CDS             7865..9061
                     /locus_tag="VHV99_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HEX3634884.1"
                     /translation="MKLIPEIQAAHGEIQTLRRTIHAHPELRYEETATADLVARTLEP
                     WGIETHRGLGKTGVVGVLKRGNRSRSIGLRADMDALPIQELNSFDHRSQNDGKMHACG
                     HDGHTAMLLGAARHLVRHCEFDGTIVFIFQPAEEGGAGAQAMIDDGLFTKFPVDAVFG
                     VHNWPGMPAGHFGVTEGPIMASSNEFRIEIKGVGSHAALPHNGRDPVFTAVQIANGLQ
                     SIITRNKKPLDTAVLSITQIHAGDAVNVVPNDAWIAGTVRTFTTETLDLIEARMRKIA
                     ESTAEAYDCSVDIHFHRNYPPTVNSSEEARFAAAVMKEIVGAENVDDAVEPTMGAEDF
                     SFMLLAKPGCYAFLGNGDGGHRESGHGAGPCMLHNASYDFNDELLSIGSTYWVRLAQR
                     FLAQEN"
BASE COUNT         1888 a         2897 c         2856 g         1933 t
ORIGIN      
        1 aaaagcaaac cgttcttccc cataatctcg tatcacctcc gtcagttcct gcaccgtggc
       61 ccgcgccagc cagtcagcag cagactcgcc gcgcgtcggg tccatccgca tgtcgagcgg
      121 gccgtcggcg cggaagctga aaccccgctc cggatcgtcg acttgcggcg acgacacgcc
      181 cagatccaac aaaacgcccg acacccgccc taccccgcgc tccgcaatcg cgtcacgcag
      241 tgaagcaaaa ctctcgtgca cgatgccaaa gcgcggatcg gcgatctgct gcgccgtggc
      301 gatggcgagc gggtctttgt cgaacgcgat caggcgcccc gcttcaccca gcttctccag
      361 caccaaccgg ctatgaccgc cgcgcccgaa cgtaccgtcc acataaacgc cgtccgcccg
      421 tgttaccagc gcattgaccg cttcttccag cagcaccgtg cgatgctgca attcgtttcc
      481 catcgcaggt gccatatttg tagtccgcga tcagaacgtg aaattcttta acgcttcggg
      541 catgccctcg gcgatcgctg cctgttcctt tgctgcgtaa atttgtgcgt cccacaactc
      601 gaagtgacga cccatgccga gcagggtcac ttccttttcc agcgagcccg cggcgcgcaa
      661 ttccggcgac acgagcaccc ggcccgcgcc gtccatatcg acgtccattg cgttgccgag
      721 aaaaatgcgc ttccaccagg cggcgttcat gggcagcttg tcgaccttgt cgcggaagat
      781 ctcccactct gggcgcggaa agagcaacag gcagccgtcc gggtgcttgg tgatcgtcac
      841 ccggccctct gcctgtgttt gcagcgcatc ccgatagcga gatggaatcg acatccgccc
      901 tttcgcatcg agcgtcagcg ccgacgcccc ttggaacaat tcgctctccc cctgtcgacc
      961 agagttagcg ctttagcgct tactctggtc ccatgcagtt tctgcggtcg accagagtta
     1021 acgcatcagc gctttactcc gtccccatcc acccggttgc tctagcggcg tttttgcgcc
     1081 ggctgcccga ttcgtgaaaa tctaccccaa accgcccgtg agatcacaca aaaatacact
     1141 ttctcacact gtctcccact ttagaggaac gctaaacagg ggtcaaggga gttacgcgct
     1201 tttcgagtgg attttgtttg taagaacaag gacttagcgc gactctctca cgtcagacat
     1261 aaaacggaaa accgttataa atgaaagagc tagtgaaact tgtgaaggta atacgtgaaa
     1321 agtgcgaggg aaaggtgcgg aatgtaagag atcgtgaagc gggattggga ggtgtttgct
     1381 ggtgttccag gtacttcgag cgaatggcgc gcgaggcgcc gtcccacttt aagacgatgc
     1441 gtgaacagag gtctcgaact tatatgtaat aggcggtccg tgtcatgact ttcgacatcg
     1501 cgcgcatcag ggctcgtgcg gggaaaggca actcgacgcc gcccgcatcc agggccgatt
     1561 taccgtgctg cgcctcgtcc acgcgcatct gttcgacgat tgcgcgcgac tcgcggtcgg
     1621 ctgcaggcaa ctcatccaga tggctatcca gatgctgttc gacctggcgt tcggtttccg
     1681 ccatgaagcc gagactcacc cggtcgccca accggcccgc cgcgaggccg atcgccagcg
     1741 cacctgtata ccagagcggg ttcaggaggc ttggacgcga atcgagcgcc tcgaggcgtt
     1801 tggcggtcca cgccagatga tcttcttcct cgatggcggc gcggttgaac acggcccgca
     1861 aggacggcga ctttgtagcg agtttctgag cctggtaaag cgcctgggcg cacacctcac
     1921 ccacatggtt cacccgcatc aagccagccg catgcgcgcg ttccgcgggc gaaagctcga
     1981 cggactccgc ggccaatcct tgcggtaccg gcagcggacg actcatccgc gacacgccgg
     2041 tcattgagcg taaaccccga tcaaactcgc taatcaactc gtccagaaac attcagtacc
     2101 ccctgattcg acaaccgtcc cgtcgcgctg acgggtccac gcgacaggct ttgcggggca
     2161 aaaccgggtt cgcgaagacc ttcggttcaa acgtttccga cggccaattt ttgattttag
     2221 accatgttgc gtaaacgaaa caggagccgt cctggccatc tcctttttct ttgtggcatg
     2281 tatgcctttt gttacattac gtgcaacttc tcgcgaagtt ggacatgcaa ggcctcaacg
     2341 ctacataaat attcgccttg tacaaaagat tcgtaattct tggagatcat tcgatgaaaa
     2401 agtcgcttct cgctcttgca gcactgggcg cattcgcagg cgtcgcacat gctcagagca
     2461 gcgtgaccct gtacggtatc attgacgaag gcttcaacat caacaccaac tcgggtggca
     2521 agcacctgta caacctgtcc agcggcgttc tgcaaggttc ccgttggggc ctgcgcggca
     2581 cggaagatct gggcggcggt ctgaaggcaa tcttcgtgtt ggaaaacggc tttgacgtga
     2641 acaacggcaa gctgggtcaa ggcggcctga tgttcggccg tcaagcgtac gtcggtctgt
     2701 cgagccagtt cggcacggtt acgatgggtc gtcagtacga ctcagtcgtc gactacgtcg
     2761 gtccgctgga agccggcgac cagtggggcg gctacatcgc tgctcacccg ggcgacatcg
     2821 acaacttcaa caacgcgtac cgcacgaaca acaccgtcaa gtacacgagc gcaaactacg
     2881 gcggcctgac gttcggcggc acgtacagct tcggcggcca ggcaggcaac ttcacgggca
     2941 accagatctg gtcgttgggc gctggctaca acaacggtcc gttggtcttg ggcgtcggct
     3001 acttgaacgc acgcaccccg gcagcatcgg gtggcctgtt caacaacggc ggcacggctt
     3061 tggcagcgaa cgcagctgtg acgtcgccgg tctacgctgg cttctcgtcg gctaatacgt
     3121 atcaggtgat cggtgctggc ggtgcataca cgttcggcgc ggcaacggtc ggcctgacgt
     3181 actcgaacat ccacttcggc gccctcggtg cgtcattcgc atcgccgctg cacaccggcc
     3241 aatcggctac gttcaacaac gcagaactca acttcaagta tcagttgacc ccggcgttgc
     3301 tggtcggcgc agcgtatgac tacacgcgcg gcgcggacat cagcggtgca tcgcgtgctc
     3361 agtaccacca aggtgctgtt ggcgtggact acttcctgtc caagcgtacc gacgtatacg
     3421 tgacgggtgt gtaccaacac gctttgggcg acacgctcaa cgcagcaggc gctacggttg
     3481 acgcaacggc cggcatcaac ggcctgacgg gctcgtcgaa ccagaatcag ttcacggctc
     3541 gcgtcggcat ccgccacaag ttctaataag tcgtaataaa gcagtttgcc tcaaggcgcc
     3601 ttcgggcgcc tttttttatg ggcgcgtgcc gcgcaggccg gctggttggt gtggaggcga
     3661 cggctcaaag agaaaggccc ggcggatgaa aatctgccag gcctttctcg cttttccgcg
     3721 caatgcgtga ctgcgtgtca gttctgttca ggtcgatggg cgtgatggac gtgcagcgcc
     3781 tgacacgctt acgcccggcc gtcgcgcaac tcgcgacgca gaatcttgcc gacattgctc
     3841 ttgggcaatt cagtgcggaa ttcgacgatc ctgggccgct tgtagccagt cagctgctgt
     3901 ttgcaatacg cgaagatgtc tgcatcggta agcgcctgat ctttcttcac gacgaacagc
     3961 tttaccgctt cacccgaatg ctgatccggc acgccgactg cggcgacttc gaatacaccc
     4021 ggcaacctag cgaccacgtc ctcgatttcg ttcggataga cgttgaagcc agagaccaga
     4081 atcatgtcct tcttccggtc gacgatcttg acgaagccat gttcattcat caagccgaca
     4141 tcgcccgatt tgaagaagcc atccggcgtc atgaccttgg ccgtttcgtc cgggcggttc
     4201 cagtagccag ccatcacctg cgggccgcgg atgcagattt cccccggctg accgagcggc
     4261 atttcgttgc cttcgtcgtc gcgaatcgag atttccgtcg acggcagcgg cagaccgatc
     4321 gtgccgctat attccgtgac ggtaaccgga ttgcaggtga cgcacggcga cgtctccgac
     4381 aaaccgtagc cctcgatgat cggcgtatgc gtatgctcga accagcgctt ggcgacggct
     4441 tcctgcaccg ccataccgcc gccatttgcc gcgatcagct tcgaaaaatc gagtttgtgg
     4501 aagtccgggc tgttcagcat cgcgttgtac agcgtgttga cggcgggaat cgtcgtgatc
     4561 ggatagcctt ccagtgcctt gatcatgccg ggtatgtcgc gcggatttgg aatcagcaca
     4621 ccgagtccgc cggtacggat cgtcagcagc ccgcataccg tcagcgcaaa gacgtgatag
     4681 agcggcagcg cgacaaccgt gatgaactga tcgatgtccg tgcggttggc gcgaaccgga
     4741 tcgagccaga tttccgactg cagtacatta gcaatcaaat tccgatgcaa cagcgtcgcg
     4801 cccttggcca cgccggtcgt accgcccgtg tactgtagaa acgcgacgtc gtccggacct
     4861 tgctggaccg gtctgaaatt ctggcgcgcg ccctcggcga tcgccgcatt gaatttgacg
     4921 tgacccggca agctacacgc cggcaccatc ttcttcacgc ggcgcacgac gaagttcaca
     4981 agcgtgccct tcacgcccat caaatcgccc attgcggcga cgacaacgtg cttgaccgac
     5041 gtgttgcgca cgatcgcctg cagcgtgaca gcgaagtttt caagcaggat gatcgcttcc
     5101 gcgccgctat ctttgagctg atgctcgagc tcgcgcggcg tgtagagcgg gttcacgttc
     5161 acgaccacat aacccgcgcg caagatcgcc gcgatcgcca cgggatattg cagtacgttc
     5221 ggcatcatga tcgcgatgcg cgcgccgcga gcgaggccct tcgactgaaa ccatgcggcg
     5281 agcttgtgcg agagcgcgtc cagttcgccg tagctgatct ctttgcccat gcagacgaac
     5341 gccggtttgg cgcggtggtc gcggaaggct tcttcgagca gctcggcgac ggacgaatag
     5401 cgggtcgggt cgatttctgc gggaacgccg ggcggataag atttcagcca gattttgtcc
     5461 atgcagcgtc tcctttttta ttttcgaatg gtcgtgctaa aaacgcatcg tagcatgggc
     5521 acccctgcgc tattcgcgaa aagcggctag gttagactgc agcctcgaac ccgttgcggg
     5581 ccgtcgctgc gagatttttg agcgtgttgt cacgcacgtt cgacttcgac aaggcagtcg
     5641 tagaacgtgg ccgatcctcc caggtccgtg agcgcctggc tggtgacctg gttggcgttg
     5701 cggccgtcgg gcgcgagctt cttccaccag atcgacaggc cgaccacgag ccctttgcgt
     5761 gccctgtcgg tgacacgggc gcgcgcttgc atcgagccgc gatcgttgaa tatgcgaacc
     5821 tgatcaccgt cgccgatgtt tcgctgcagc gcgtcgaccg ggtggatgtc cagatgcggc
     5881 tcgccttccg tcgagcgcag actttcgatg ttcacgaagg tgctgttcag gaagttgcga
     5941 gcaggcggcg aaatcatcgc aagcgggtaa cgggcggcga gctcgggcgc accgtccgcg
     6001 gactcgtaag gcggcagata atccggcagc gggtcgagac cttgttgcgc gagacgctcg
     6061 ctgaaaaact cgcacttacc ggacggcgtg cgaaagccac cctcggcgaa cggcgcgtca
     6121 ggcagcttta gctccgccca gccggttttc tttagcgagt cccagcttac gccttcgagc
     6181 gtcttgtcat tccagcggaa ggctacctgt gcgatagcgt cgtcgctatc gtagagcgct
     6241 ggctcctgca ggcccatgtg gcgcgcgagg ccgcgaaaga tctccgtgtt cgggcgtgca
     6301 tcgccgaccg gcgcgatcgc cggcaagttg accatcacgt gtgtgtgccc gtacgacttg
     6361 tgcacgtcga ggtgctcgag ttgggtcgtg gccggtagaa gcaaatcggc gtagtcagcg
     6421 gtgtcggtcc gaaaatgttc gagtacgatc gtgaacaggt catcgcgtgc aaaccctgct
     6481 gcgacccgtt ccgagtccgg cgcgaccgcc accggattgg agttgtagac gatgatcgct
     6541 tcgacttttg gaccgaaggt ggcgtcgccc gggtgcagca gcgcgtcgcc gatcgcgttc
     6601 atattaatga cgcgcgaagc cttggcgggc cagccgggca tcagatccgg tcgctgcaat
     6661 gcgtgcgaat ccaccggcgc ccatccgccc gaggacagca ggacaccacc cgcccgctcg
     6721 cgccatgctc ctgtcagcga aggtaagcag gcgattgcgc gtacggcgtt gccaccgcca
     6781 cgcacccgct gcagtccgta attcatccgg atcgcggcct ttttcgtgct gccgtaaagg
     6841 cgcgcaacat cgacaatcac ctgctcgtcg acaccgcaaa tttgagcgac gcgggagggt
     6901 ggataactga gcgcgcgcac cttgagttgc tcgaagccca atgtatgagc cgcgatgtac
     6961 gcgtgatcga gcagattttc cgtgatcaac acgttgatca tgccgagcgc caacgcaccg
     7021 tcggttccgg gtttaagtgc aatatgctgg tggcattttt ccgccgtcag cgatcggtat
     7081 ggatcaatcg cgatcagccg tgcaccgcga cgcttggctt cctgagcgcg tgtccagaaa
     7141 tgcaggttcg acgcgatggg attcgaaccc cagatcagga caacctcgct ttcggcgaaa
     7201 aactcggtgc gcatgccgag gctggcgccg taggtgtact tcaaccccgc cgcaccagcg
     7261 gctgcgcaga tcgtgcggtc taactgtgat gctccgagtt tgtggaagaa ccgctgcgca
     7321 atgctgtcgc cctgaaccag gcccattgtg ccggcgtagc tgtacggcat gatggcttct
     7381 ggcgcccggc tcgcaatctc cgccagacgt actgcggcca attcgaacgc ctgatcccag
     7441 ctgaccggct caaaacggcc ttcgcctttg cggccgatgc gcttcattgg tgtcgtcaag
     7501 cgatgcggat ggtgaacccg gtcagcgtag cggctgactt tggtacacag cgcaccttga
     7561 gtcggcggat gatcgggatc gccaacgacc ttgaccgcca gtccgccttc gatagtcacg
     7621 cgcatcgcac aggtatcagg gcaatcgtgc gggcacacgg cgcgggcgaa ttcagttgga
     7681 gcattcatcg gcgatcgtcc ggaagaacgg taaacggcgg ggcaatcccg gattttatta
     7741 cgtccgatgc cccttgacct gcactgtgcc gccaaaaatg ccttcggcgt agaatcggtt
     7801 atcaacgtgc ccgggcaata tggccccgag cgattccacg caacgcaaag cacaagcgcc
     7861 catcatgaaa ctgatcccag aaatccaggc cgcccacggc gaaattcaga ccctccgacg
     7921 aacgattcat gcccaccccg agttgcgtta cgaagaaacc gcgacagcgg atctggttgc
     7981 caggacactt gagccctggg gcatcgaaac tcatcgtgga ttgggcaaaa caggcgttgt
     8041 cggcgtactg aaacgcggca acagatcgcg ctcgatcggg ctacgcgccg acatggacgc
     8101 gctgccgatt caggagctaa acagcttcga tcatcgttcg cagaacgacg gcaaaatgca
     8161 cgcgtgcggc catgatggac ataccgcgat gctgctcggc gcagcgcggc atctggtcag
     8221 gcactgcgaa tttgacggca cgatcgtgtt catctttcaa ccggcagagg aagggggggc
     8281 cggcgcacag gcaatgatcg acgatggcct gttcacaaag tttcccgtcg atgcggtttt
     8341 cggcgttcac aattggccgg gcatgccggc aggccatttc ggcgtgaccg aaggcccgat
     8401 catggcgtct agcaacgagt ttcgcattga aatcaaaggc gtgggttcac acgcggcgct
     8461 gccgcacaac ggccgcgacc ctgtgttcac agcggttcag attgcgaacg ggttgcagag
     8521 catcatcacg cgaaacaaga agccgctcga taccgcagta ctgtcgatca cccagattca
     8581 cgcaggcgac gccgtcaatg tcgtcccgaa cgatgcgtgg atcgctggca ccgtgcggac
     8641 ttttaccacc gaaacactcg atctgatcga agcacgcatg cgcaaaatcg ccgagagcac
     8701 ggccgaggct tacgattgtt cggtcgatat ccacttccat cgcaactatc caccaacggt
     8761 caacagtagt gaagaggccc gcttcgcggc ggcggttatg aaggaaattg tcggcgcgga
     8821 aaatgtcgac gacgcagtcg aaccgacgat gggcgccgag gatttttcgt tcatgctgct
     8881 ggcgaagccg ggctgttacg catttctcgg taatggcgac ggcgggcacc gggaatcagg
     8941 gcatggtgcc gggccttgca tgctgcataa cgcgagctat gacttcaacg acgagctatt
     9001 gtcgatcggt tcgacgtatt gggtgcggct ggcacaacgg ttcctcgcac aggaaaatta
     9061 agtggtctcg gtgggctgaa atcggatgcg cttgtttgga gctgacgggt tacacgagcg
     9121 cgctgcggat cgggaaggag tgacgccaga ggaacggcag cgtactggag cggaccgcac
     9181 gacaacgatg ttccagatca ggccattcga tgtttgcgga gccggccgtt gtgacggaca
     9241 gagcggcgga cagttgcggg gacgcggtcc cggtccgggc gtgagcgtgc ccgccgtcgc
     9301 ggtgatgacg gcgggggcgg agcacgcggt ctgcacccgt ttgcgggggc gttctttgcc
     9361 tgtgccgtgt gcccgttccg gcgctgcttt gcccgttttt tgcgtttgcc cgtttttttc
     9421 ggggcggtaa acgcagaaac cccaccttct gggggtgggg tttctgttgc tgctggggag
     9481 cctgacgatt acctactttc acacgggaat ccgcactatc atcggcgtgg agtcgtttca
     9541 cggtcctgtt cgggatggga aggggtggga ccga
//
LOCUS       DATASH010000183         4617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5958202,
            whole genome shotgun sequence.
ACCESSION   DATASH010000183 DATASH010000000
VERSION     DATASH010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4617
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5958202"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="VHV99_05260"
     CDS             complement(<1..965)
                     /locus_tag="VHV99_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3634885.1"
                     /translation="MPSLQWFTELTQRERRTLYAGFGGYAVDAFDFMIYSFLIPTLIA
                     TWGMTKSEAGMIATSSLISSAIGGWLAGILADRYGRIRVLQWTIATFAIFTCLSGFTH
                     SFWQLLTTRTLQGIGFGGEWSVVTIMMAETIRSPQHRAKAVGTVQSSWSFGWGAAAII
                     YWAFFALLPEQYAWRACFWIGLLPALWIIYIRRNVSDPDIFLETRRARESGFDTSHFL
                     QIFSGAHLKTTLLGSALCTGMLGGYYAITTWLPTYLKTVRHLSVFNTSGYLIVLIVGS
                     FMGYIVGAILCDKIGRRASFVLFAIGSFVLGMVYTMLPITDGMMLA"
     gene            1218..2144
                     /locus_tag="VHV99_05265"
     CDS             1218..2144
                     /locus_tag="VHV99_05265"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HEX3634886.1"
                     /translation="MPATPKTCSPSSRFASKPLRADQSRRAFLKSSVSVLLLSGMSSS
                     LLSACGGGNLDADQPATPRLASVNNFRDVAGAATGYPTVDGKQMRRGAFYRANALTPS
                     AADAAALVKLGIVSVYDLRTPGEISRTADILPAGATLQALDVLGTSDFVAPTFDTAGA
                     AVAFMEAQARAYVTGAAQRIGYGELLTRLANGAGAQLFHSDAGKDRAGWVAALLLSIA
                     NVPLDVIMQDYLLSNAYLAQSIQAQAATLRAQDGDAVATAESPLFNVEENYLQAGFDQ
                     VQASYGTMANYLTQGLGMSQATIDTLHSRLVV"
     gene            complement(2181..3452)
                     /locus_tag="VHV99_05270"
     CDS             complement(2181..3452)
                     /locus_tag="VHV99_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3634887.1"
                     /translation="MRNPLNTLFGRMALLSTTVLFAIQACWFVLVVMQPPRHEIDGFA
                     RGLLLVLQAINGEPMKGAALAPAMRVHLVPTWNMPSSVHLHAPTVHPLVELSRHLREN
                     LPPGTQIAVDDLRPPHLWVLFPGKSNWVVVPVDLPPRPRFLIESISMLLAALLLSLFA
                     VWQMQRPLSRVADAARAFGSGGRPEPVTVQGPRELRDLIGSFNDMMRRLNEAGDDQAV
                     MLAGVAHDLKAPLTRLKLRASVLVAENERAGLIRDVDSLTNIVQQFLEFAGQSADAGP
                     PIEVDAFLQEQFSASDSTETPLFALDLRAGSSFTLPRTLLDRLVTNLVDNALEHGSPP
                     VDIATSRNDRHWVISVRDHGPGIPDERVAAAMKPFVRLDAARGGEGHCGLGLAIVARL
                     AHDRGGRCHVRNHAQGGLEVRIELPVGPTLT"
     gene            complement(3449..4189)
                     /locus_tag="VHV99_05275"
     CDS             complement(3449..4189)
                     /locus_tag="VHV99_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006052257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3634888.1"
                     /translation="MNPQVLIVDDDPVVRDLLCKFLQTNGFDASVLHDGTHLQRRLER
                     ERPSVVVLDIMMPNTDGLRALTALRAAGDDIPVIFVTARGTVADRIVGLSLGADDYLT
                     KPFDPRELLARIHTVLRRRGPSTTSAPEARKPYRFGPFELDFASRALSRDGSKLPLRD
                     SEFALLKIFVNNPYKVLSRVLIHDLVHRDNLAFRDRSLDVPIWRLRRVIEDDPSNPCY
                     VQTVRGKGYVFVPDADGTPFADETAANA"
     gene            complement(4281..>4617)
                     /locus_tag="VHV99_05280"
     CDS             complement(4281..>4617)
                     /locus_tag="VHV99_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX3634889.1"
                     /translation="RFGRFEVNFPARELHHDGERIALRSSEFAMLKVFVTHAMTVLTR
                     AQLLEKLHGNSEVHRNRSLDVSIWRLRRLIEVDPSQPRYVQTVWGRGYVFVPDGEIGA
                     VDRSAPLLT"
BASE COUNT          883 a         1453 c         1477 g          804 t
ORIGIN      
        1 gcgagcatca tgccgtcggt gatcggcagc atggtgtaga ccatgccgag cacgaacgag
       61 ccgatcgcaa acagcacgaa cgacgcgcgc cggcctatct tgtcgcacag aatcgcgccg
      121 acgatatagc ccatgaacga gccgacaatc agcacgatca gatagccgct ggtgttgaag
      181 accgacagat ggcgcacggt tttcagatag gtcggcagcc aggtggtgat ggcgtagtag
      241 ccgcccagca tgccggtgca gagcgcgctg ccgagcaagg tggttttgag gtgggcgccg
      301 gagaagatct gcaggaaatg tgaggtatcg aagccacttt cccgagcgcg gcgcgtttcg
      361 agaaaaatgt cgggatcgct gacattgcgg cggatgtaga tgatccacag cgcgggcagc
      421 aggccgatcc agaagcaggc gcgccatgcg tactgctccg gcagcagcgc gaagaacgcc
      481 caatagatga tggccgccgc gccccagccg aaggaccagc tgctctgcac ggtgccgacg
      541 gctttcgcgc gatgctgcgg tgaacggatg gtttcagcca tcatgattgt caccaccgac
      601 cattcaccgc cgaagccgat gccttgcagc gtgcgggtag tgagcagttg ccagaacgag
      661 tgcgtgaagc cggacaggca ggtgaagatc gcgaacgtgg caatcgtcca ctggagaaca
      721 cggattcgtc cgtagcggtc ggcgaggatg ccggcgagcc agccgccgat cgccgacgaa
      781 atcagcgaac tggtcgcgat catgccggct tcgcttttgg tcatgcccca tgtcgcgatc
      841 agcgtcggaa tgaggaatga gtagatcatg aaatcgaaag cgtcgactgc gtagccgccg
      901 aagccagcat aaagggtgcg gcgttcgcgc tgggtcagtt cggtgaacca ttggagagaa
      961 ggcatgttat tttgtggtct ccagctgttt ttttgccttt cggcggcttg attgtgggtt
     1021 ttttgtattc taaggggacg aggtgttgtg ccgccagttg tggtcaaaaa tggggacggc
     1081 gcaaaaacgc cattcctgtt ccttaccaac tactaccgtg taacccaaaa atcgttgacc
     1141 gtcgttaaac tgcagcggca gcagctcagg ctgcgacaat cctcttcaca cccgcgctcg
     1201 ccacctcgcc accgctcatg cccgcaaccc caaagacctg ctcgccgtcc agccgcttcg
     1261 cgagcaagcc tttgcgggca gatcaatcgc gccgtgcgtt cctgaaatcg tcggtaagtg
     1321 tgctgctgct ctccggtatg agcagttcgc tgctgtcggc ttgcgggggc ggaaatctgg
     1381 acgctgatca gccggcaacg ccacgcctcg cgtcggtgaa caactttcgc gatgtagcgg
     1441 gtgcagccac cggctatccc actgtcgacg gcaagcagat gcgccgcggc gccttctatc
     1501 gcgcgaacgc gttgaccccg agcgccgccg atgcggccgc cctcgtcaaa ctcggcatcg
     1561 tctcggtcta cgatctgcgc acgcccggcg aaatctcgcg caccgcggat atcctgccgg
     1621 ccggcgcgac attacaggcg ctcgacgtcc tcggcaccag cgacttcgtc gcgcccacgt
     1681 tcgacacggc aggcgccgcc gtcgcgttca tggaagccca ggcgcgcgcc tatgtcaccg
     1741 gcgctgccca gcgcatcggc tacggcgaac tcctcacacg gctcgccaac ggcgccggcg
     1801 cgcaactgtt tcattccgac gcgggtaagg accgtgccgg ttgggtcgcg gctctcctgc
     1861 tcagcatcgc caatgtgccg ctcgacgtca tcatgcagga ctatttgctg agcaatgcct
     1921 atctggccca gtcgatccag gcacaggccg ccacgctgcg tgcgcaggac ggcgacgccg
     1981 tcgccacggc cgagtcaccc ttgttcaacg tagaggaaaa ttatctgcag gcgggtttcg
     2041 atcaggtgca ggcgagctac ggcacgatgg ccaactatct gacgcaaggc ctgggcatgt
     2101 ctcaggcgac catcgatacc ttgcacagcc gtctggtcgt ctgacgcctg ctcggcgcgc
     2161 tcagcccttc tcgaccattt tcacgtcagc gttggcccga ccggcaattc gatccgtact
     2221 tcgaggccgc cttgcgcatg attgcgcaca tggcagcgtc cgccgcgatc gtgcgcgagc
     2281 cgcgcgacga tcgcgagacc gaggccgcaa tgaccttcgc cgccacgtgc ggcgtcgagc
     2341 cgtacgaacg gcttcattgc cgcggcgacg cgctcgtcgg gaatgcccgg gccgtggtcg
     2401 cgcacgctga ttacccaatg ccgatcattg cgcgacgtgg cgatatccac cggcggcgag
     2461 ccgtgctcga gcgcattgtc gacgaggttc gtgaccagcc ggtcgagtaa ggtgcgcggc
     2521 agcgtgaacg acgaacccgc gcgcagatcg agcgcgaaca gcggcgtctc ggtgctgtcg
     2581 ctggcggaaa actgttcctg caggaaggcg tcgacttcga tcggcggccc agcgtcagcg
     2641 gactgaccgg caaactccag aaactgctgg acgatgttgg tcaatgagtc gacgtcgcgg
     2701 atcagtccgg ctcgctcgtt ttccgcgacc agtacgctcg cgcgcaattt gagccgcgtg
     2761 agcggcgctt tcagatcgtg cgcgacgccg gccagcatca ccgcctggtc gtcgccggct
     2821 tcgttcaggc gccgcatcat gtcgttgaac gaaccgatca gatcgcgcag ctcacgcgga
     2881 ccctgcaccg tgacgggttc cggccggccg cccgaaccga acgcgcgagc cgcatccgcc
     2941 acgcgggaca gtggccgctg catctgccag accgcgaaca gcgacagaag cagcgcggcc
     3001 aacaacatcg aaatggattc gatcaggaag cgcgggcgcg gcggtaggtc caccggcacg
     3061 acgacccagt tcgacttgcc gggaaacagc acccataaat gcggcggacg caggtcgtcg
     3121 acggcgatct gcgtgccggg cggcaggttt tcccgcagat gccggctcaa ttcgaccagc
     3181 ggatgcacgg tcggcgcgtg cagatggacg ctcgacggca tgttccaggt gggcacgagg
     3241 tgcacacgca tggcgggcgc gagcgcggcc cctttcatcg gttcgccgtt gatcgcctgc
     3301 agcaccagca gaaggccgcg cgcgaagccg tcgatttcgt ggcgtggcgg ctgcatcacc
     3361 accagcacga accagcatgc ctgaatcgcg aacaggaccg ttgtcgacag caacgccatt
     3421 ctgccgaaca gcgtgttcag cggatttctc atgcgtttgc ggcggtctcg tcggcgaacg
     3481 gcgtgccgtc cgcgtcgggt acgaacacgt agcctttgcc gcgtacggtc tgcacgtagc
     3541 agggattgga cggatcgtct tcgatcacgc ggcgcaggcg ccagatcggc acgtcgaggc
     3601 tgcggtcgcg gaaggcgaga ttgtcgcgat gcacgaggtc gtgaatcagc acacgcgaca
     3661 gcactttgta cggattgttg acgaagatct tcagcaacgc gaactcgctg tcgcgcagcg
     3721 gcagcttcga gccgtcgcgc gacagcgcgc ggctggcgaa atcgagttcg aacgggccaa
     3781 agcgatacgg cttgcgagct tccggcgcgc tcgtggtgga cggcccacgg cggcgcagta
     3841 ccgtatggat gcgcgcgagc agctcgcgtg gatcgaacgg cttggtcagg tagtcgtccg
     3901 cgccaagcga aagcccgacg atgcggtcgg ccaccgtacc gcgcgccgtc acgaagatca
     3961 ccggaatatc gtcaccggcc gcgcgcaacg cagtgagcgc gcgcaggccg tcggtgttcg
     4021 gcatcatgat gtcgagcacg acgaccgacg gccgctcacg ttcgagccgg cgttgcagat
     4081 gggtgccgtc gtgcagcacc gacgcgtcga agccgttcgt ctgcagaaac ttgcaaagca
     4141 gatcgcgtac gaccgggtcg tcgtcgacga taaggacctg tggattcatg gcgaaattct
     4201 agactggcac gtgtgacgcg cgaccgactg ttttcttacc aatgcttacg cagccgcacg
     4261 gtgggaagtc tggacaaatc ttacgtgagc agcggcgcgc tacggtccac cgcgccgatt
     4321 tcaccgtccg gcacgaacac gtagccgcgt ccccagacgg tttgcacata gcggggctgg
     4381 gacggatcga cttcgatcag ccgccgcaaa cgccagatcg acacatcgag gctgcgattg
     4441 cgatgcacct cgctattgcc atgcagcttc tccagcaact gcgcgcgcgt gaggacggtc
     4501 atcgcgtgcg taacgaacac cttgagcatc gcgaattcgc tcgagcgcag cgcaatgcgc
     4561 tcgccgtcgt ggtgcaattc gcgcgctggg aaattcactt cgaaacggcc gaagcga
//
LOCUS       DATASH010000184         2125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_815439,
            whole genome shotgun sequence.
ACCESSION   DATASH010000184 DATASH010000000
VERSION     DATASH010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_815439"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2125)
                     /locus_tag="VHV99_05285"
     CDS             complement(<1..>2125)
                     /locus_tag="VHV99_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HEX3634890.1"
                     /translation="TDMSRDQTTRATTVFDRTLLRAGETVSMKHLMREETLSGFAFPS
                     HLPTRVTIRHEGSGQTYHLPLKWAADRSADSSFPIPAAAKLGEYSVTLDDGSTTSDDN
                     NDSDNSGSGHMSIETGNFRVEEFRLPVFKGSIGIRDQKTGALVAATTAPLAVQIEYVS
                     GGPASNLPVQVSALMKNTSPSFASQFESFSFAPYKKPADDGASAPATEGDDNSNAQED
                     TSTAKLIADKTALTLDRNGTGTLTLKSLPVVEAPKQLALEATFADPNGEIQTLRGSAT
                     LWPAAVAAGIKAGDWVSVGNAVPVQALAVDLQGKPRAGVALEIRGVARTMTSSRKRMV
                     GGFYAYDNHTETKDLGTLCSGKSDDHGLLSCEAKLKEAGNVDLIVTAKDGDGRVANAT
                     TSVWVTREDQLWFGGENTDRIDVLPEKTSYEPGETARFQVRMPFRFATALVAVEREGI
                     VETKVVELDGKNPTVELKVQESWGPNVYVSVLAIRGRIHEVPWYSFFTWGWKAPLDWV
                     HAFWYEGRQYEAPTPLVDLSKPAFRYGLGEIKVGTASHKLGVTVTPDAKSYAVRTKAH
                     VKVRVTLPGGKPAPAGTQIALAAVDEALLELMPNQSWNVLDAMLQRRAYGVETSTAQM
                     EIIGRRHFWRKAVPAGGGGGHSATRELFDTLLLWNPRVTLDANGEATVDVPLNDALTS
                     FRIVAIGAVGAGQFGTGNASIRSTQD"
BASE COUNT          350 a          691 c          643 g          441 t
ORIGIN      
        1 gtcctgcgtg ctgcgtatcg acgcattgcc ggtgccaaac tgacccgcgc ccacggcccc
       61 gatagcgaca atgcgaaagc tcgtcaacgc gtcgttgagc ggcacgtcta ctgttgcttc
      121 gccgttcgca tcgagcgtca cgcgcgggtt ccagagcaag agcgtatcga acagttcgcg
      181 tgtcgcgcta tgtccgccgc caccgcccgc gggcacggct ttacgccaga agtgacggcg
      241 tccgatgatt tccatttgcg ccgtcgatgt ctccacgcca tacgctcgcc gttgcagcat
      301 ggcgtcgagt acgttccagc tttgattcgg catcagttcg agcagggctt cgtcgacggc
      361 ggcgagcgcg atctgcgtgc cggccggcgc gggcttgccg cccggcagcg tcacgcggac
      421 tttcacatgc gcctttgtac gtacggcgta ggatttcgca tccggtgtaa cggtcacgcc
      481 gagcttgtgc gaggccgtcc ccaccttgat ctcgccgagc ccgtaacgga acgcaggctt
      541 cgacaaatcg accagcgggg taggcgcttc atattgccgc ccttcgtacc agaacgcgtg
      601 cacccaatcg agcggcgctt tccagcccca cgtgaagaat gaataccacg gcacctcgtg
      661 aatccgcccg cgtatcgcga gcacggagac atacacgttc ggcccccacg actcctgtac
      721 tttcagctcg acagtcgggt tcttgccatc gagttccacg accttcgttt ccacgatacc
      781 ctcgcgctcc accgccacca gcgcggtcgc gaagcggaac ggcatgcgca cctggaagcg
      841 ggcggtttcg ccgggttcgt agctggtttt ttcaggcagc acgtcgatcc ggtccgtgtt
      901 ttcgccaccg aaccagagtt gatcctcgcg cgtgacccag accgacgtgg tcgcattggc
      961 cacgcgccca tcgccatcct tggcagtgac aatcaggtcg acgttgcccg cttccttgag
     1021 ctttgcctcg caggaaagca ggccgtgatc gtcgctcttg ccgctgcaga gcgtgccgag
     1081 gtccttggtt tccgtgtggt tgtcatacgc gtagaaaccg ccgaccatgc gcttgcgcga
     1141 gcttgtcatg gttcgcgcaa cgccgcggat ttcgagtgca acacccgctc gcggcttgcc
     1201 ttgcaagtcg acggcgagtg cctgcaccgg caccgcgttg ccgaccgaca cccagtcgcc
     1261 cgccttgatg ccggcagcaa cagcggcggg ccagagcgtg gcgctgccgc gcagcgtctg
     1321 gatttcgccg ttagggtccg cgaaggtcgc ttccagtgcc agctgcttcg gcgcttccac
     1381 caccggcagc gacttcagcg tgagggttcc cgtgccattg cggtcgagcg tcagcgcggt
     1441 tttatcggcg atgagcttgg ccgtggacgt gtcttcctgc gcgttgctgt tgtcgtcgcc
     1501 ctcggttgcg ggcgcgctgg cgccgtcatc ggcaggcttc ttgtacggtg cgaagctgaa
     1561 cgattcgaac tgcgaagcaa aggacggcga cgtatttttc atcagcgccg acacttgcac
     1621 cggcaggttc gatgccggtc cacccgacac atattcaatc tgtaccgcca gcggcgccgt
     1681 agtcgcagcc accagcgcac cggtcttttg atcgcgaatg ccgatcgatc ccttgaacac
     1741 cggcaggcgg aattcttcaa cgcggaaatt gccggtttcg atactcatgt ggcccgatcc
     1801 ggaattgtcg ctgtcgttgt tgtcgtccga ggtggtcgaa ccatcatcca gcgtcacgct
     1861 gtattcgccg agcttcgcgg cggctggaat cgggaagctt gaatccgcgc tccggtcggc
     1921 tgcccatttg agcggcagat gataggtctg gccgctgccc tcatgacgaa tcgtcacgcg
     1981 cgtcggcaaa tgagaaggaa acgcgaaacc gctcagcgtt tcttcgcgca tcaaatgttt
     2041 catcgatacc gtttcgcccg cgcgcagcag cgtccggtcg aacacggtgg tggcgcgcgt
     2101 ggtttgatcg cggctcatat cggtg
//
LOCUS       DATASH010000185         4806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3782629,
            whole genome shotgun sequence.
ACCESSION   DATASH010000185 DATASH010000000
VERSION     DATASH010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3782629"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VHV99_05290"
     CDS             complement(<1..597)
                     /locus_tag="VHV99_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3634891.1"
                     /translation="MTQQSVRIDYVCHGIGRVELRRFDPSRDSYVALTALLHRAFTRL
                     GAMGLNCTCVDQTVETTRSRATRGDCYVAVCGGRIVGTMTLYAPDHDSPCELYRRDDI
                     ASLRQFGVEPMWQARGIGTLLIAFADHWAATRGYAELALDTPQPAAHLIAFYRGQGFR
                     IVDFVRFDGKRYDSAILSKPPVVTRTLANWSHRLTVPAR"
     gene            complement(853..1755)
                     /locus_tag="VHV99_05295"
     CDS             complement(853..1755)
                     /locus_tag="VHV99_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX3634892.1"
                     /translation="MSRDLDSSLLRAFVTVAETGAVGVAAVRLARTQAAVSMQLRRLE
                     EELGCRLLDRSPRGVQLTEAGHLLLPFAHTILGASADARRALSAGQVSGTVRLGMLED
                     IAVGRLPRALRRFSIAYPQVALEIVVDTSPALSNRLAEGGLDVVVGDPALVDSVPLLT
                     WTQPLFWVGARGFSRDGDAALPVVAFGGACLWQQQVLTALRRAGIAWRIVCTSTSLPA
                     VQSAVEAGLGVSVLLDGNIRSESMRVLGQADGLPDAPRADFGLFMRPVAGAQAAAVQT
                     LQTFLCEELHLGLRESEPVVSFAG"
     gene            1859..2749
                     /locus_tag="VHV99_05300"
     CDS             1859..2749
                     /locus_tag="VHV99_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX3634893.1"
                     /translation="MQDTTIKPALNSAPAPVKPASAAAGAALLCLLSMSSVQFGAALS
                     APTMAAYGSLSTTWLRLCWAAVVLALLVRPRFFTYSRSHWFAAGALGAAMAGMTLCFF
                     AALQRIPLGLAVAIDFLGPLSVATFAVRRARALLWPVLAIGGVLLLSRDRVGWIGEPL
                     GVLLAFGAALGWGSYIVLMKKTGTVFAGLEGLSVSLIAAALVATPFGLAQGGIHIAAG
                     QIAATAGLAVLVPLLPYALEMVALRHMPAASFGILMSLEPAIGALAGFVVLHQPMGVP
                     QIAGTLLVVAASVGAVVATR"
     gene            complement(2799..3350)
                     /gene="rfbC"
                     /locus_tag="VHV99_05305"
     CDS             complement(2799..3350)
                     /gene="rfbC"
                     /locus_tag="VHV99_05305"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEX3634894.1"
                     /translation="MGNKVMATALPEVKLIEPEVFCDEFGFCFESFSADEFTDDVSHG
                     FTFVQDRHLRAVRGVLRGLHYQVQRPQGRLVRVVVGEVFDVAVDVRLNSPTFGKWSSA
                     YLSAANHRQVWVPPGFAHGFVVLSEVAECLWKTTEYWFPELERCILWRDPDIGIEWPI
                     DVEPILGAKDAAGRRLYEAENIA"
     gene            complement(3501..4496)
                     /locus_tag="VHV99_05310"
     CDS             complement(3501..4496)
                     /locus_tag="VHV99_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3634895.1"
                     /translation="MPHMHLDTARITWLHTPTLNVRTAARRIGVLLFDGFWLLGPGTV
                     VEMFQTANELSGSRAGEEPPYEVQFLSMNGGSVASSSLARIWTDRIDPRYGSGFDVLF
                     IAGGYGAHEAALDERLLGWLRSVQTRTRAIETIGEGRLVLEAAGPAEHDGLQVNRYLG
                     SAAIDPTRSAANDRNDCARSALMFIKRDLGAELARSVADRVMPGMAATWVPLPAEGGT
                     LSVAEKIRAAARWMEANCDRPVSVADAAQVAAMSERNFLRRFKHEMQVTPSDYLLQVR
                     LRIACNFLTETELPVDKIARRSGTGNGDRLAKIFRKRMALSPTEYRARSRVASEA"
BASE COUNT          936 a         1603 c         1426 g          841 t
ORIGIN      
        1 cctcgccggg acagtcaatc tatgcgacca gttcgcgagc gtacgtgtca ccaccggcgg
       61 cttgctcagg atcgcgctgt cgtatcgctt gccatcgaaa cgtacgaaat cgacgatgcg
      121 aaacccctgc cctctgtaaa acgcaatcag atgcgcggcc ggttgcggcg tatcgagcgc
      181 aagctccgca taaccgcgcg tcgcggccca atgatcggcg aaagcgatca acaaggtacc
      241 aatgccccgt gcctgccaca tgggctccac gccgaactgg cgcaagctgg cgatatcgtc
      301 gcgacgatac aactcgcacg gcgaatcatg atccggcgca tacagcgtca tcgtgccgac
      361 aatgcgcccc ccgcaaaccg cgacgtagca atcgccccgc gtcgcgcgtg aacgtgtcgt
      421 ctccaccgtt tgatccacac aggtgcaatt caaccccatc gcgccgagtc gtgtgaaagc
      481 ccgatgcaac agcgcagtca acgcaacgta cgaatcgcgc gacggatcaa accggcgcag
      541 ttcgacacgg ccgatgccgt gacacacgta gtcgatacgg acggattgct gcgtcattgc
      601 acgtcgcttc acgttgagca ccttgcgcta tcaagatgca cgaagtgtag gtttcacgcg
      661 taagcgtctc aagaaaaaat gtcgtaaaaa ctcacatgag acgcagtgcc gatgtgccga
      721 tgtgccgatg tgccgatgta ccgatgtacc gatgtaccga tgggccaatg ggccaatggg
      781 ccaacggacc aacgtgccaa tggacaaatg gaccaacatg acatcacgtt gcccgcagaa
      841 acggcccacg cgctaccccg cgaacgacac aaccggctca ctctcccgta acccgagatg
      901 caattcctcg caaagaaacg tctgcaatgt ctgcacggcc gcggcctggg cgccggccac
      961 cggccgcatg aaaagcccga aatcggcgcg gggcgcatcg ggcaatccat cggcctgacc
     1021 gagcacgcgc atcgactctg aacggatatt gccgtcgagc agcaccgaca caccaagccc
     1081 tgcttccacg gccgactgca ccgccggcaa actggtactg gtgcatacga tgcgccacgc
     1141 aatccctgcg cgccgcagcg ccgtcaacac ctgctgctgc cacaagcacg cgccgccgaa
     1201 cgcaaccacc ggcaacgccg cgtcaccatc gcggctaaac ccgcgcgcgc cgacccagaa
     1261 gagcggctgc gtccaggtca gcagcggcac tgagtcgacc agtgcggggt cccccaccac
     1321 tacgtcgaga cccccttccg caagccggtt cgacaatgcc gggctggtat cgacaacgat
     1381 ctccagcgcc acctgcggat aagcgatcga aaaacgccgc agcgcgcgcg gcagccgacc
     1441 aacggcgatg tcttccagca tgccgagccg cactgtgccg gacacctgcc ccgcgctcaa
     1501 cgcccgccgc gcatcggcgc tcgcgccgag gatcgtatgg gcgaacggca gcagcagatg
     1561 ccccgcttcg gtgagttgca cgccgcgcgg cgagcggtcg agcaaacggc accccagttc
     1621 ttcctcgagc cggcgcaatt gcatgctcac cgccgcctgg gtccgcgcga ggcgcacggc
     1681 cgccacaccg acggcgccgg tctccgccac cgtgacgaac gcgcgcagga gactgctgtc
     1741 gagatctcgg ctcatcacgt ttcctgaagt ggccataaga aatatcaact tatcttaatg
     1801 ccgcccatac cgataagata gcgtccacga accagaaaag cgggaccgag aggccacgat
     1861 gcaggacacc accatcaaac cggcgcttaa ttccgcgcca gcaccagtga agccggccag
     1921 cgcggcggcg ggtgccgcac tcctatgttt gctgtcgatg tcgagcgtgc agttcggcgc
     1981 cgcgctctcc gcgccgacaa tggcggcata cggctcgctc agcaccacct ggttgcgcct
     2041 ttgctgggca gccgtggtgc ttgcgttgct ggtgcgcccg cgtttcttca cctactcgcg
     2101 ctcgcattgg tttgcggccg gtgcgttggg cgcggcaatg gccggcatga cgctgtgctt
     2161 tttcgcggcg cttcagcgga ttccactggg gcttgccgtc gcgatcgatt ttctggggcc
     2221 gctttccgta gcgactttcg cggtccggcg cgcgcgagcg ctactgtggc cggtattggc
     2281 gattggcggc gtgctgttgc tctcgcgaga tcgcgtgggc tggatcggcg aaccgcttgg
     2341 cgtcctgctc gcgttcgggg ccgcgctcgg ttgggggagc tatatcgtct tgatgaaaaa
     2401 gaccggcacc gtgttcgccg gactcgaagg attgtcggtg tcgttgatcg ccgcggcgtt
     2461 ggtcgccacg ccattcggcc tcgcgcaagg cggcatccac atcgcggcag gccagatcgc
     2521 cgccactgcg gggctcgcgg tgctggtccc gttgttaccc tacgcgctgg aaatggtggc
     2581 attgcggcac atgccggccg catcgttcgg gatactgatg agtctggaac ccgcgatcgg
     2641 cgcgttggcc ggcttcgtgg tgctgcatca accaatgggt gtgccgcaga tcgcaggcac
     2701 gctgctggtg gtcgccgcga gtgtgggtgc cgtagtggca acacgctgaa cggcactcaa
     2761 tcgctgcggg ttcgatgcgg gtgcgggtgc cgccgcgctc aggcgatatt ttccgcttcg
     2821 tagagacgcc ggcccgctgc gtccttcgcg ccgaggatcg gctcgacgtc aatgggccac
     2881 tcgataccga tatcagggtc gcgccacaag atgcagcgct cgagttccgg gaaccagtat
     2941 tcggtggtct tccagaggca ctcggcgacc tcggacagca cgacgaaacc gtgcgcaaag
     3001 cccggcggca cccacacctg ccgatgattg gccgcgctca gataggcgct gctccacttg
     3061 ccgaaggtcg gcgaattgag ccgcacgtcc acggcgacgt cgaacacctc ccccactacc
     3121 acgcgtacga gccgaccttg tgggcgctgc acctgatagt gcagcccgcg caacacacca
     3181 cgcactgccc gcaaatgacg gtcttgcacg aaagtgaacc cgtgcgaaac gtcgtcggta
     3241 aactcatccg cgctgaaact ctcgaagcag aagccgaatt cgtcgcaaaa cacctcgggc
     3301 tcgatgagtt tcacctctgg caatgcggtc gccattacct tgttgcccat ggccactgtg
     3361 ctcgtaagaa gcctgaccgg agaaaacaca gccggtgatg aacacgccgt tgcacacgct
     3421 attacgcacg cccctacgca cgtcgctgcg cacgtaccca tccacgcaga agtgtgccgt
     3481 ccgcacggcg ctccccaagc ttacgcttcg gacgccaccc ggctgcgcgc gcgatattcg
     3541 gtgggcgaca acgccatgcg cttgcggaat atcttcgcga ggcgatcgcc gttgccggtg
     3601 ccgctgcggc gcgcgatctt gtcgaccggt agttcggttt ccgtcaggaa gttgcaggca
     3661 atccgcaacc gcacctgcaa caggtagtca gacggcgtga cctgcatttc atgcttgaag
     3721 cgccgcagga aatttcgctc gctcatcgcc gcgacctgtg cagcgtcggc aaccgacacc
     3781 ggacgatcgc aattcgcttc catccagcgc gcggcggcac ggattttctc ggcgacgctg
     3841 agcgtgccgc cttcagccgg cagcggcacc caggtggcgg ccatgcccgg catcacccga
     3901 tcggcgacgc tgcgcgcgag ctccgcgccg aggtcacgct tgatgaacat cagcgcgctg
     3961 cgcgcacagt cgttacgatc attggcggcg ctgcgtgtgg gatcgatcgc ggcgctgccc
     4021 agatagcggt tcacctgcag gccgtcatgc tccgcgggcc ccgccgcctc cagcacgaga
     4081 cggccttcac cgatcgtctc gatcgcgcgg gtacgcgttt gcaccgagcg cagccacccg
     4141 agcagccgct cgtcgagcgc ggcctcatgt gccccgtatc cgcctgcgat gaacagcacg
     4201 tcgaaaccgc tgccgtaacg tgggtcgata cggtccgtcc agatccgcgc caatgacgaa
     4261 ctcgcgacgc tgccgccgtt cattgacagg aactgcactt cataaggcgg ttcttcgccg
     4321 gcccgcgaac ccgagagttc gttggcggtc tggaacatct cgaccaccgt gccgggcccg
     4381 agtaaccaga agccgtcgaa cagcaacacg ccgattcgcc gcgctgcggt acgcacgttg
     4441 agcgtcggcg tatgtaacca ggtaatcctc gcagtatcca ggtgcatgtg cggcatgatg
     4501 tttccccaaa ctgtcgtacg ttgtggaccc gctgccccgt cggctggccg atatggtcga
     4561 acttaatcag gccggtgata accgctggca acgccgctta acggattaag cgtgatgtta
     4621 gctgaatgct tcgctatagt aaatttgaaa aacaaatcca tatcgggaaa tgcaatgtat
     4681 gaagaaaacc attattgtgc gtttcatatg actgccccgt atatgaccgg accgtgccat
     4741 caatgatatc ggaatggcgc cggtcacgag ccctgaaaac cttacacgca ctagctttca
     4801 agcctg
//
LOCUS       DATASH010000186         1531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2398654,
            whole genome shotgun sequence.
ACCESSION   DATASH010000186 DATASH010000000
VERSION     DATASH010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2398654"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /gene="mraY"
                     /locus_tag="VHV99_05315"
     CDS             <1..1038
                     /gene="mraY"
                     /locus_tag="VHV99_05315"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX3634896.1"
                     /translation="IRKLTAMKIGQAVRSYGPQSHLLKAGTPTMGGVLILLGIAVSTL
                     LWADLTNRFVWIVMLVTFGYGVIGWVDDYRKVVYKDPRGMSSREKYFWQSVIGLFAAV
                     YLAFSVSEANNVRVFDLFMAWVRSGLSMGLPARADLMLPFVKSISYPLGVWGFIVLTY
                     LVIVGASNAVNLTDGLDGLVIMPVVLVGASLGVFAYVMGSSVYSKYLLFPHIAGAGEL
                     LIFCSAMGGAGLAFLWFNTHPAQMFMGDVGALALGGALGTVAVIVRQEIVLFIMGGIF
                     VAETLSVMLQVTWFKFTKRRYGEGRRLFKMAPLHHHFELSGWKETQVVVRFWIITLML
                     CLFGLSTLKLR"
     gene            1062..>1531
                     /locus_tag="VHV99_05320"
     CDS             1062..>1531
                     /locus_tag="VHV99_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX3634897.1"
                     /translation="MFGEKFRDRQKPMVLVLGLGESGLAMARWCARHGCRLRVADTRE
                     VPPNLSALQAHSVDAEFVGGPFSPVLLEGVELVAISPGLSPLAADLLPLITAAREQGI
                     PVWGELEFFAQALKTLGESGYAPKVIAITGTNGKTTTTSLTGLLCERAGKKVAVA"
BASE COUNT          238 a          427 c          526 g          340 t
ORIGIN      
        1 atccgcaagc tgaccgcgat gaagatcggc caggcggtgc gcagctacgg tccgcaatcg
       61 catctgctca aagccggtac gccgaccatg ggcggcgtgc tgatcctgct tggcattgca
      121 gtgtcgacgt tgttgtgggc cgacctgacg aaccgtttcg tctggatcgt gatgctggtc
      181 accttcggct acggcgtgat cggctgggtc gacgattacc gcaaggtggt ctacaaagac
      241 ccgcgcggta tgtcgtcgcg tgaaaagtat ttctggcagt cggtgatcgg gctgttcgcg
      301 gccgtgtatc tcgcgttcag cgtgtccgaa gcgaacaacg tgcgcgtgtt cgacctgttc
      361 atggcgtggg tgcgcagcgg cttgtcgatg ggcttgccgg cgcgcgccga cctgatgctg
      421 ccgttcgtca aatcgatcag ctatccgctc ggcgtgtggg gcttcatcgt gctgacgtat
      481 ctggtgatcg tcggcgcgag caatgcggtc aatctcaccg acggtcttga cggcctcgtg
      541 atcatgccgg tcgtactggt tggcgcatcg ctcggcgtgt tcgcttatgt gatgggtagc
      601 tcggtgtact cgaagtacct gctgtttccg catatcgcgg gtgcgggcga gttgctgatc
      661 ttctgttcgg cgatgggtgg ggcagggctc gcgttcctct ggttcaacac acacccggca
      721 cagatgttca tgggcgacgt cggcgcattg gcgctgggcg gcgcgctcgg cacggtggcg
      781 gtgatcgtgc gccaggaaat cgtgctcttc atcatgggcg gcattttcgt cgcggagacg
      841 ctctccgtga tgttgcaggt gacgtggttc aagttcacca agcgccgtta cggcgagggc
      901 cggcgtctct tcaagatggc gccgctgcat caccactttg aattgtcggg ctggaaggaa
      961 acgcaggtgg tcgtgcgttt ctggatcatc acgttgatgt tgtgcctgtt cggattgtcc
     1021 acgctcaagt tgcgttaagt cgtatttagg ggaagcaggc gatgtttggc gagaagtttc
     1081 gggatcggca aaagccgatg gtgctcgtgc tgggactcgg tgaatccggt ctcgcaatgg
     1141 cgcgctggtg cgcgcggcac ggctgtcggc tgcgtgtggc cgatacgcgc gaggtgccac
     1201 cgaacctgtc cgcgctgcaa gcgcacagcg tcgatgctga attcgtcggc ggcccgtttt
     1261 cgccagtgtt gctcgaaggc gtggagcttg tggcgattag cccgggcctg tcgccgctcg
     1321 ccgccgactt gctgccgctg attacggcag cgcgcgagca aggcatcccc gtgtggggcg
     1381 aactcgaatt cttcgctcaa gcgttgaaga cgctcggcga aagcggctac gcgccgaagg
     1441 tgatcgcgat caccggcacc aacggcaaga ccacgaccac gagcctgacc ggtctcctgt
     1501 gcgagcgcgc cggcaagaag gtcgcggtgg c
//
LOCUS       DATASH010000187         2949 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5365841,
            whole genome shotgun sequence.
ACCESSION   DATASH010000187 DATASH010000000
VERSION     DATASH010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5365841"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(360..>2949)
                     /locus_tag="VHV99_05325"
     CDS             complement(360..>2949)
                     /locus_tag="VHV99_05325"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3634898.1"
                     /translation="GHLELAARENMESGLAPREAQTAARHEFGSIALAEEVTRDMWGG
                     RWFVDLLQDVRYGLRMLRKSPGFAAIAILTLALGIGANTAIFSVVYAALLRPLPYFQA
                     NCLFTLSEARPGVRDPYWASSYPNYQDWTKQSRTFQAMAGFADDGFVFRGGAEPQQVT
                     ASMVTPNFFSTLGVKPMLGYDFPTNEDIAANAPSVALLTYGFWRTQFGGDPNIVGRSI
                     QLSDSSVTIIGVLPRSFEFAPMGAAQIWVPLHLGSSFSSFTQRRNFGWLRVIGRLAPG
                     VTAAQAHAEMNIISTRLAAAYPQADVAMQADMVPLRDRISGQVQSLLWILFGAVGFVL
                     LIACANVADLLMVRALGRKREFAIRAALGASRGRLISQLLTESLLLALAGAALGFLAG
                     QWGTVLLVDAIPHSLIDNMPFLRDTQPNAAIIAFLCGVAVLTGVLFGLAPAMQISRKK
                     VSDTLKEESRTASGGARTWLRDALVVGEIALSLVLLAGAGLAAKSLSALLHRNPGFDT
                     QNLVTFTVILPRESYPKDSDYVRFDKAFTSQLRGEPGIVSVGNTNITPLTGGGGGTLR
                     FVIEGQPVQPGHEDVAAMRRVSSDYFSTMKIPLIEGRFFSDEADSATAPKHVIVNQAW
                     VRAYLPGEDPIGKRLRSAASAAQPYQEIVGVVGNTALGQLDSAESPAVFYPFDQGPYG
                     YVNYIVRAAGNPAIVPTEVRDALSRTDQQLFPVQLLTMEQIIQQSPSVFMRRYPSYLI
                     GSFAALALLLASIGLYGLISYSVSQRTREIGIRVALGAQQGDVMRMVMGQGARLTLIG
                     VLIGMVAALGLTQLMSSILYGVKATDPLTFASVSTLLAIVALAASYVPARRVMRVDPI
                     AALRYE"
BASE COUNT          645 a          943 c          842 g          519 t
ORIGIN      
        1 aaaaatgatc gagcggcgga tcgacacgac tacagcgcct cagcataccc gcggctcgca
       61 aggtcgtacg ataacgcatc ccctactcat atctcaaggc cacaatgggg tccacctttg
      121 tcgcgcgccg cgccggaatg tagcaggcaa gcagcgcgac tccgcgctcc cggctggcgc
      181 ggacagagcg gcccgtttcg cgcaatccag tccccacgcg aacgccgagg tatttgagag
      241 ccggcatcga gccgagccaa aagacatgcc accagcgtga cgcgcaaacc ggcaactcac
      301 atactacgtc ccatcgcgcc catttttcat gcgccgttgt cgggccgcag tacggtgatt
      361 tactcgtaac gcagcgcggc gatgggatcg acacgcatga cgcggcgcgc gggaacatag
      421 gaggcagcga gcgccacgat tgccagcaag gtggacacgc ttgcaaacgt caacgggtcc
      481 gtcgctttca cgccatacag tatgctgctc atcagttgcg tgagtccaag cgcggccacc
      541 ataccaatca gcacaccaat cagcgtgagc ctggcacctt gccccatcac catccgcatg
      601 acgtcaccct gttgcgctcc cagcgccacg cgaattccga tctcgcgcgt ccgctgcgaa
      661 acggagtacg aaatcaatcc ataaagcccg atcgacgcca gcagcaacgc cagcgcagcg
      721 aagctgccga taagatatga cggatagcgc cgcataaaaa ccgacggcga ctgctggatg
      781 atctgctcca tcgtgagcag ttgcacaggg aaaagctgct ggtccgttct cgacagcgca
      841 tcacgcactt cggtcggcac aatcgccgga ttgcccgctg cgcgcacgat gtaattgacg
      901 tatccgtatg ggccctgatc gaacgggtag aaaaccgcgg gcgattcagc gctatcgagc
      961 tgcccgagag cggtgtttcc taccacgccg acaatttcct ggtacggctg cgccgccgac
     1021 gcagctgacc ggaggcgctt gccgatggga tcttcgccgg gcagataggc tctcacccat
     1081 gcctgattga cgatcacgtg tttcggcgcg gtggcggagt cggcttcatc gctaaaaaag
     1141 cggccctcta tcagaggaat tttcatggtg gaaaagtaat ccgatgaaac ccgtcgcatg
     1201 gcggccacgt cttcgtggcc tggttgcaca ggctggccct cgatgacgaa gcgcaacgtg
     1261 cctcctcctc cgccggtcag gggagtgatg ttggtatttc ccacgctgac gatgcccggt
     1321 tcgccacgca gctgtgaggt gaaggccttg tcgaaccgaa cgtaatcaga gtccttggga
     1381 tacgactcgc gcggcaaaat cacagtaaac gtgacgaggt tctgcgtgtc gaaccccgga
     1441 ttgcggtgaa gcagcgcgct caggctcttg gcggctaggc cagcgcctgc cagcagcacc
     1501 agcgaaagcg caatttcgcc caccacaagc gcgtcgcgca gccaagtgcg tgctccaccg
     1561 ctcgctgtgc gcgattcttc tttgagcgta tcgctcactt tttttcgcga gatttgcatc
     1621 gccggcgcga ggccaaacag cactcccgtc agcaccgcaa cgccgcacag aaatgcgatg
     1681 atcgcggcat tcggctgcgt gtcgcgtaaa aacggcatgt tatcgatcag gctatgcgga
     1741 atcgcgtcca ccagtaggac ggtaccccac tgcccggcca gaaatcccag cgcggcgccg
     1801 gccaaagcga gcagcaagct ctccgtgagc aattgcgaaa tcaaacggcc gcggctcgct
     1861 ccgagcgccg cgcgaatcgc gaattcccgt ttgcgcccca acgcgcgcac catcagcaaa
     1921 tccgccacat tcgcgcacgc aatcagcagt acaaatccta ccgcgccaaa aagaatccat
     1981 aataatgact gcacttgtcc gctgatccgg tcgcgcaacg gaaccatgtc ggcttgcatc
     2041 gcgacatcgg cttgcggata cgcagcggcg agacgcgtgc taataatgtt catttcggcg
     2101 tgcgcctgcg ccgcagtgac gccaggcgca agccgcccga tgacgcggag ccatccgaag
     2161 ttgcggcgct gcgtgaaaga actaaaactg ctgccgaggt gcagcggcac ccagatttga
     2221 gcagccccca tgggcgcgaa ctcaaaactc ctcggcagca cgccgataat cgtcacgctg
     2281 ctatcgctca actgaatgct gcgcccgacg atattcggat cgccgccaaa ttgcgttcgc
     2341 cagaaaccgt atgtcagcag cgctacgctg ggcgcgttgg cagcgatgtc ttcgttcgtc
     2401 ggaaaatcgt agcctagcat cggcttcacg ccaagcgtgg agaaaaaatt cggcgtgacc
     2461 atgctggcgg tgacctgctg cggttcagca ccgccgcgaa acacaaaccc atcatcagcg
     2521 aatcccgcca tcgcctggaa cgtccgcgat tgcttcgtcc aatcttgata gttgggatac
     2581 gacgacgccc aatacgggtc ccgcaccccg ggtcgagctt cgctaagcgt gaagaggcaa
     2641 ttagcctgaa aatacggtag cggccgcaga agcgcggcgt aaaccacgga gaagatggcg
     2701 gtgttggcgc caatgccgag agcgagggtc agaatggcaa tggcggcgaa gccgggagat
     2761 ttgcgcagca tgcgcagacc gtagcgaacg tcttgcagca ggtctacaaa ccagcgccca
     2821 ccccacatgt ctcgcgtcac ctcctcagcg agcgcgatac ttccaaattc gtggcgcgcc
     2881 gctgtttggg cctccctcgg tgccaagccc gattccatgt tctctctcgc ggcgagttcc
     2941 aagtggcct
//
LOCUS       DATASH010000188         3056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2596376,
            whole genome shotgun sequence.
ACCESSION   DATASH010000188 DATASH010000000
VERSION     DATASH010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2596376"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..718
                     /gene="kynB"
                     /locus_tag="VHV99_05330"
     CDS             80..718
                     /gene="kynB"
                     /locus_tag="VHV99_05330"
                     /EC_number="3.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylformamidase"
                     /protein_id="HEX3634899.1"
                     /translation="MDTLWDITPAVDTATPVWPGDTPVGIERVWRMEAGSPVNVARLT
                     LSPHTGAHADAPLHYDAEGAAIGHVALDAYLGRCRVIHCIGASPVVTPQHLTTSLDDL
                     PPRVLLRTYKNAPTSAWDSAFCAVAPETIDLLAARGVKLIGIDTPSLDPQESKTMDAH
                     HRIRAHRMAILEGIVLDAVAPGDYELIALPLKLTTLDASPVRAVLRALPESR"
     gene            799..2049
                     /gene="kynU"
                     /locus_tag="VHV99_05335"
     CDS             799..2049
                     /gene="kynU"
                     /locus_tag="VHV99_05335"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004550795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HEX3634900.1"
                     /translation="MNTREEALALDSADPLATLRDQFALSATTIYLDGNSLGAPPAAA
                     AQRAQTVIAAEWGEGLIRSWNNAGWFELPRRLGNKLAPLIGAADNEVVVTDTISINLF
                     KLLSAAVRVANARDPKRRVIVSERSNFPTDLYIAQGLIEQLDRGYELRLVDDPSELPA
                     AIGDDTAIAMITHVNYRTGYMHDMAALTTLIHDKGALALWDLAHSAGAVPVDLNEVGA
                     DYAVGCTYKYLNGGPGSPAFVWVPKRHQNEFAQPLSGWWGHRAPFKMDPAYQPDDGIG
                     RFLCGTQPMVSMSLVECGLDVFLQTDMQTIRAKSLALTDLFIELVEARCSEFPLTLVT
                     PREHAQRGSHASFEHPHGYEVMQALIARGVIGDYREPRVLRFGFTPLYTRFVDVWDAV
                     ETLREVLAQETWRAPEFAARGAVT"
     gene            2060..3037
                     /gene="kynA"
                     /locus_tag="VHV99_05340"
     CDS             2060..3037
                     /gene="kynA"
                     /locus_tag="VHV99_05340"
                     /EC_number="1.13.11.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase"
                     /protein_id="HEX3634901.1"
                     /translation="MTDHMQTPGLPEEKPAQGCPFGYGSGGARVASVASAASAASAAS
                     ASDASVSSNDGWHDAQLDFSESMSYGDYLSLGTVLDAQHPLSPDHNEMLFIIQHQTSE
                     LWMKLALYELRAALKAVHRDELPPAFKMLARVSRIMEQLVQAWSVLATMTPSEYTAMR
                     PYLGSSSGFQSYQYRQIEFLLGNKNEQMLKPHAHRPDVLAEVTASLQAPSLYDEVVRL
                     LSRRGFEISPSRLERDWTQPTTHDASVEAAWLEVYRNPSQHWELYEMAEELVDLEDAF
                     RQWRFRHVTTVERIIGFKQGTGGTSGAPYLRKMLDVVLFPELWHVRTML"
BASE COUNT          545 a          893 c          976 g          642 t
ORIGIN      
        1 agaaggagat tttttcgcaa tcacatttcg ccgcaagcca cctatcattc ccggatagac
       61 acaatccgcc gatggagaca tggacacact ctgggacatc acccccgccg tcgataccgc
      121 gacgcccgtc tggcccggcg atacgccagt cggcatagaa cgcgtgtggc gcatggaggc
      181 gggctcgccc gtcaacgtcg cgcggctcac gctgtcgccg cacaccggcg cccacgccga
      241 tgccccgctg cactacgacg ccgaaggcgc ggcgatcggc cacgtcgccc tcgatgcgta
      301 ccttggacgc tgccgcgtga ttcattgcat cggcgcgtcg cctgttgtga cgccacaaca
      361 cctgaccact tcgctcgatg acctcccgcc ccgagtctta ctacgcactt acaaaaacgc
      421 accgacttcg gcgtgggaca gcgccttctg cgcagtggcg cccgagacaa tcgatctgct
      481 cgctgccagg ggtgtgaaac tgatcggcat cgacacaccc tccctcgatc cgcaggaatc
      541 gaagacgatg gacgcgcatc atcggattcg cgcgcatcga atggcgatcc tcgagggcat
      601 cgtgctggac gccgtcgcgc ccggcgacta cgaactgatc gcgctgccgc tgaaactgac
      661 cacgctcgac gcgagccccg tgcgcgccgt gttgcgcgcg ctgcccgagt cccgctgatc
      721 gccgaggctc actcaggaaa accgcatcac gcattcatcg catccgccga actccttgat
      781 ctgattgaca ggaccaccat gaacacccgt gaagaagcat tggcgctcga tagcgccgac
      841 ccgctcgcga cattacgcga tcagttcgcg ttgtcggcga ccactattta cctcgacggc
      901 aattcgctcg gcgcgccgcc ggccgccgcc gcgcagcgcg cgcaaaccgt catcgccgct
      961 gaatggggtg aaggtctgat ccgcagctgg aacaatgccg gctggttcga actgccgcgc
     1021 cgcctcggca acaagctcgc gccgctgatc ggcgcggcgg acaacgaagt agttgtcacc
     1081 gatacgattt cgatcaatct gttcaaactg ctctcagccg ctgtgcgggt ggcgaatgcg
     1141 cgcgatccga agcgtcgcgt gatcgtctcc gagcgttcga atttcccgac cgatctgtac
     1201 atcgcgcaag ggctgatcga acagctcgac cgcgggtatg aattgcgcct cgtcgacgat
     1261 ccgtccgaac tgcctgccgc gatcggcgac gacaccgcga tcgcgatgat cacgcatgtg
     1321 aattaccgca ccggctatat gcatgacatg gccgcgctga ccacgctgat tcacgacaag
     1381 ggcgcgcttg cgctgtggga cctcgcgcat tcagcgggtg cggtgccggt cgatctcaat
     1441 gaggttggcg cggattacgc ggtgggctgc acgtataagt atctgaatgg cggtccgggt
     1501 tcgcccgcgt tcgtgtgggt gcccaagcgc catcaaaacg aatttgcgca accgttgtcc
     1561 ggttggtggg gccatcgcgc gccgttcaaa atggacccgg cgtatcagcc cgatgacggt
     1621 atcggccgct ttttgtgcgg cacgcagccg atggtgtcga tgtcgctggt cgaatgcggg
     1681 ctcgatgtgt ttctgcaaac cgatatgcag acgatccgtg cgaagtcgct tgcgctgacc
     1741 gacttgttta tcgaactggt cgaggcgcgt tgcagcgagt ttccgttgac gctcgtgacg
     1801 ccgcgcgagc atgcgcagcg tggttcgcat gcgagcttcg aacatccgca tggttatgag
     1861 gtcatgcagg cgttgatcgc gcgtggtgtg atcggtgatt atcgtgagcc gcgtgtgttg
     1921 cggtttggtt ttacgccgct gtatacgcgt tttgtcgatg tgtgggacgc ggtggagacg
     1981 ttgcgcgagg tgcttgcgca agagacctgg cgggcccctg agtttgccgc tcgcggcgct
     2041 gtgacctgag gagcgcgcga tgaccgatca catgcaaacg ccgggactgc cggaagagaa
     2101 gccggcgcaa gggtgtccgt ttgggtatgg gagtggcggt gctcgggtgg cctctgtggc
     2161 ctctgctgcc tctgctgcct ctgctgcctc tgcttccgat gcttcagttt cgtccaacga
     2221 cggctggcac gacgcgcagc tcgatttttc ggagtcgatg agttacggcg attatctgtc
     2281 gttggggaca gtgctggatg cacagcatcc tttgtcgccg gatcacaacg agatgctgtt
     2341 catcatccag catcagacga gcgagttgtg gatgaagctc gcgctgtatg agttgcgcgc
     2401 agcgctgaaa gcggtgcatc gcgatgaatt gccgccggcg ttcaagatgc ttgctcgcgt
     2461 gtcgcgaatc atggaacagc tcgtgcaggc atggagtgtt ctcgcgacga tgacaccgtc
     2521 cgagtacacg gcgatgcggc cttatctggg cagctcttcc gggttccagt cttatcagta
     2581 tcggcaaatc gagtttttgc tcggcaataa gaatgagcag atgctcaagc cgcatgcgca
     2641 tcggccggat gtgcttgcgg aggtgacggc ttcgctgcaa gcgccttcgc tttacgatga
     2701 agtggttcgg ttactctcgc ggcgcgggtt tgagatttca ccgtcgaggt tggagcggga
     2761 ttggacgcag ccgactactc acgatgcttc ggttgaggcg gcgtggttgg aggtgtatcg
     2821 gaatccttcg cagcattggg agctgtatga gatggcggag gagctggtcg atctcgagga
     2881 cgcgtttcgg cagtggcggt ttcggcatgt gacgacggtt gaacggatta ttgggttcaa
     2941 gcaaggtacg ggtgggacga gtggcgcgcc gtatttgcgg aagatgctgg atgtggtttt
     3001 gtttcccgag ctttggcatg tgaggacgat gctttaacaa gcgaagcggg ccttcg
//
LOCUS       DATASH010000189         4511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_24577,
            whole genome shotgun sequence.
ACCESSION   DATASH010000189 DATASH010000000
VERSION     DATASH010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4511
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24577"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1090
                     /gene="hflX"
                     /locus_tag="VHV99_05345"
     CDS             <1..1090
                     /gene="hflX"
                     /locus_tag="VHV99_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX3634902.1"
                     /translation="SAGANPLVTLTGRRSSPDAKMFVGSGKAEELRLACEANDIELVI
                     FNHALAPAQQRNLEQALNRRVIDRTSLILDIFAQRARSHEGKLQVELAQLQYLSTRLI
                     RAWTHLERQKGGIGLRGPGETQLETDRRLIGERIKALKTRLEKLRRQHGTQRRARSRN
                     QTMSVSLVGYTNAGKSTLFNALTKAQAYAADQLFATLDTTSRRVYLGDEVGQVVVSDT
                     VGFIRELPHQLVAAFRATLEETIHADLLLHVVDASSAVRLDQIDQVNEVLHAIGADTI
                     RQVLVFNKIDAVPELAARGDAVERDEYGNISRVFLSARTGQGLDTLRAAIAEIATAES
                     LPDTLVDQSEEDRSAAPRDDHKVTELGH"
     gene            1140..2534
                     /gene="hflK"
                     /locus_tag="VHV99_05350"
     CDS             1140..2534
                     /gene="hflK"
                     /locus_tag="VHV99_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007580430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HEX3634903.1"
                     /translation="MNDYNERSIWLRMRAMLSLNDPRWGRGDGNGERQRPNEPKRPPA
                     KDGEGPPDLDEMWRDFNRRLSRVFGRKGGGAGGGRPDNGRGARIGMGIVIGVLIAIYL
                     GSGVFVVQDGQAGVVMQFGKYRYTAGQGVHWRLPYPFEAHELVNIGQIRQVEIGRNNV
                     VRLANVKDASMLTHDADIVDVRFAVQYQVSKPTDYLFRSVDPDQSVMQAAQAAVRSIV
                     GARSTNDILYQDRETIRQQLVASIQQSLDEYQSGLTVTGVTIQGVQVPGQVQAAFDDA
                     AKVRQDNERAKRDAQAYAAELLPRAQADVARRIDEAKTYSDKTVAQAQGDAERFKQVY
                     AQYLKAPAVIRERMYLDTMQQIYSNTTKVFVDSKSGNNVLYLPLDKLVEQTRQRAADA
                     AAAAAASGAAAASGAAVPQAMSSTPAPSAAAPSAAASTPAAVATPASQAAASSDALRS
                     RDSFRSRMREDDVQ"
     gene            2547..3449
                     /locus_tag="VHV99_05355"
     CDS             2547..3449
                     /locus_tag="VHV99_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HEX3634904.1"
                     /translation="MNKIIALVVAVVIVLFAASSMVFVVDQRHMAVLSSHGDTAPVLL
                     GPGLHVKLPPPLQTVTLVDSRIQSLDAPDEDRYVTSDKTDLLANPVVKYRVTDPLKLL
                     AETRGDVQSLADRLALLSRSALGDAFAKVTLTDALAKQQTVADEARGTMDKAAASLGV
                     SVLDLQLTRVDFPAAMADAVYKRMIAAREQTAADERAKGTAEADQIKADAVGQQQAIL
                     ADGYRQAQTIKGEGDAKAAEIAAEAYGSDPQFYQFYQSMQAYKNTFKPGDVIVVDPSS
                     EFFRFMRSSTGAAAPDAPAAPRKH"
     gene            3493..3684
                     /locus_tag="VHV99_05360"
     CDS             3493..3684
                     /locus_tag="VHV99_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009973312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HEX3634905.1"
                     /translation="MDIAGSLLLAIALMLIIEGMFPFVFPSAWRETFRKIAERPSHQI
                     RIGGLIVMVLGLILLFIVT"
     gene            3907..>4511
                     /locus_tag="VHV99_05365"
     CDS             3907..>4511
                     /locus_tag="VHV99_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEX3634906.1"
                     /translation="MSTWLLPENIADVLPSEARKIEELRRHLLDRFRSYGYEMVMPPL
                     LEYLESLLTGGGHDLNLRTFKLVDQLSGRTLGLRADITPQVARIDAHLLNRQGVTRLC
                     YAGNVAHTRPRGLHATREQIQIGAEIYGHAGLEADLEIQQLMLDALRLAGLAKVRLDL
                     CHAGVLAALIEAEPAAAELGQSLYDALAGKDVPRLVELTADL"
BASE COUNT          853 a         1432 c         1420 g          806 t
ORIGIN      
        1 aagcgcgggc gcgaatccct tagttaccct cacggggcgc cggtccagtc ccgatgccaa
       61 gatgttcgtc ggcagcggca aggccgaaga actgcgtctc gcgtgtgagg cgaacgacat
      121 cgaactcgtc attttcaatc acgctctggc ccctgcgcag cagcgcaatc tggagcaggc
      181 gcttaaccgc cgcgtgattg accgcaccag cctcattctc gatatttttg cgcaacgcgc
      241 ccgcagccac gaaggcaagc tgcaggtcga gctcgcgcag ttgcagtact tatccacgcg
      301 gctaatccgc gcatggaccc accttgaacg ccagaaaggc ggtatcggtt tgcggggccc
      361 aggcgaaaca cagctcgaaa ctgaccgccg tttgatcggc gagcgcatca aggcgctcaa
      421 aacccgcctc gaaaagctgc gccgtcagca cggcacgcag cgccgcgcgc gcagccgtaa
      481 ccagaccatg tccgtgtcgc tcgtcggcta tacgaacgcg ggcaaatcca cgcttttcaa
      541 tgcgctgacc aaggcccagg cgtatgccgc cgaccagctg ttcgccaccc tcgatacgac
      601 gtcccgccgc gtctatctcg gcgacgaagt cggccaggtg gtggtgtccg acacggtcgg
      661 tttcatccgc gaattgcctc accaactggt agcggctttt cgcgccacgc ttgaggaaac
      721 cattcacgcc gacctgctgc tgcatgtggt cgacgcgtcg agcgccgtac gcctcgatca
      781 gatcgatcag gtgaacgagg tgttgcacgc aatcggtgcg gacaccatcc gccaggtgct
      841 ggtgttcaac aagatcgacg ccgtgccgga gttggcggcc cgtggcgacg cggtcgagcg
      901 ggatgagtat ggtaatattt cgcgcgtctt tttgagcgcg cgtacggggc aagggctgga
      961 tacgttgcgc gctgccatcg ctgaaatcgc tactgccgaa tctcttcccg atacgttggt
     1021 cgatcagtcg gaagaagacc ggtcggcagc accgcgcgac gaccacaagg tcacagaact
     1081 cgggcactga cccgctccaa ttgcactgac ccgctgtcta ctctggtgaa cgaacacagg
     1141 tgaacgatta caacgagcgg agtatctggc tgcgcatgcg cgccatgctg tcactgaacg
     1201 atccgcgctg gggccggggc gacggcaatg gcgagcgcca acgacccaac gaacccaagc
     1261 gtccgccggc caaggacggt gaaggcccgc ccgatctcga cgagatgtgg cgcgatttca
     1321 accgccgttt gagccgggta ttcggccgca agggcggcgg tgcgggtggt ggccgtccgg
     1381 ataacggtcg cggcgcgcgc atcggcatgg gcatcgtgat cggcgtgttg attgcgatct
     1441 atctcggcag cggcgtgttc gtcgtgcaag atggccaggc tggcgtcgtg atgcagtttg
     1501 gcaagtatcg gtataccgca gggcagggcg tgcattggcg cctgccgtat cctttcgagg
     1561 cgcacgaact cgtcaatatc ggccagatcc gccaggtgga gatcggccgc aacaatgtgg
     1621 tgcgtctcgc caatgtgaag gacgcgtcga tgctcacgca cgacgccgat atcgtcgacg
     1681 tgcgctttgc cgtgcagtat caggtgagca agccgaccga ctatctcttc cgtagcgtcg
     1741 atcccgatca gagcgtgatg caggctgcgc aggcggcggt gcgcagcatc gtcggcgcgc
     1801 gcagcacgaa cgacatcctc tatcaggatc gcgaaacaat tcgccagcaa ctggtggcgt
     1861 cgattcagca atccctggat gaataccagt ccggcctcac cgtgaccggt gtgacgattc
     1921 agggcgtgca ggttcccggc caggtgcaag ccgctttcga cgacgcagcc aaagtgcgtc
     1981 aggacaacga gcgcgcgaag cgtgacgcgc aggcctatgc cgcggaactg ctgccgcgcg
     2041 cgcaagccga cgtcgcgcgc cggatcgacg aagcgaaaac gtatagcgac aagaccgtgg
     2101 cccaggcgca aggcgacgct gagcgcttca agcaggttta cgcgcagtac ttgaaagcgc
     2161 cagcggtgat tcgcgagcgc atgtacctgg ataccatgca gcagatctat tcgaatacaa
     2221 ccaaggtgtt tgtggacagc aagagcggca acaacgtgct gtatctgccg ctcgacaagc
     2281 tggtcgagca gactcgccag cgcgccgctg acgctgccgc ggccgcggcc gcttcaggtg
     2341 ccgccgctgc gtcgggtgcg gccgtgccgc aagccatgag cagcactccc gcgccatctg
     2401 ctgccgcacc gtctgccgcc gcgtccacac ctgccgcggt tgcaacgcct gccagccagg
     2461 cggcagccag cagcgacgca ctgcgttcac gcgactcctt ccgcagccgt atgcgcgaag
     2521 acgacgttca ataaggagcg cacaccatga acaagatcat tgcgctcgtc gttgccgtcg
     2581 tcatcgtgct gttcgccgcg tcgtcgatgg tattcgtggt cgaccagcgg cacatggccg
     2641 tgctgtcgtc gcatggcgac accgctcccg tattgctagg ccccggcctg catgtgaagc
     2701 tgccgccgcc gctgcaaacg gtcacgctgg tcgatagccg catccagtcg ctcgacgcgc
     2761 cggatgaaga ccgttatgtc acgtcggaca agaccgatct gctggccaac ccggtcgtca
     2821 aatatcgtgt gaccgatccg ctgaagctgc tcgccgagac ccggggcgac gtgcaaagcc
     2881 tggcggaccg cctggcgctg ctgtcccgta gcgcgcttgg cgacgcgttc gccaaggtca
     2941 cgctgaccga tgcgcttgcg aagcaacaaa ccgttgctga cgaagcacgc ggcacgatgg
     3001 acaaagccgc ggcatcgctg ggcgtttccg tgctcgacct gcaactgacg cgcgtcgact
     3061 tcccggcggc aatggccgac gcggtctaca agcggatgat cgccgcgcgc gagcagaccg
     3121 cggccgacga acgcgcgaag ggcacggccg aggccgacca gatcaaggcg gacgctgtcg
     3181 gccagcaaca ggcaattctc gcggacggct atcgtcaggc gcagaccatc aagggcgagg
     3241 gcgatgccaa ggcagcggaa atcgcggccg aggcctatgg cagcgacccg cagttctacc
     3301 agttttatca aagcatgcag gcctacaaga acaccttcaa accgggcgac gtgatcgtgg
     3361 tcgacccgag cagcgagttc ttccgcttca tgcgtagttc gaccggcgcc gccgccccgg
     3421 acgctcccgc ggcgccgcgc aaacactaat aaccggaacg ccgcggcatc cgcatcgcgg
     3481 cccttcattc gcatggacat agccggctcg ttactgctcg cgattgcatt gatgctgatt
     3541 atcgaaggga tgtttccctt cgtttttccg agcgcctggc gcgagacgtt ccgtaaaata
     3601 gcggagcggc cgtcgcatca gattcgcatc ggcgggctga tcgtgatggt gcttggattg
     3661 attctgctgt ttatcgtgac ttgaagggcg ctgggcggcg cccaccgccg cgcgccgtcg
     3721 cattgatagc gacattgcgt ggcaaccacg ggccttgcgg cacgggccaa agtcgctgaa
     3781 gggccgaagg tgccggaagg cggttccgat gctcgcgaca gccgccaaaa tagcgccgcc
     3841 gcccgccatt cacactcact taccggcgca cccagctgcc gtgttcaacg tcgtaggact
     3901 gtatcgatgt cgacctggtt acttcccgag aatattgccg acgtgctgcc gtcggaagcc
     3961 cgcaagatcg aagagttgcg gcgccatttg ctggaccgtt tccgctcgta cggctacgag
     4021 atggtgatgc cgccgttgct cgagtatctc gagtcgctgc ttaccggggg cgggcacgat
     4081 ctgaatctgc gcactttcaa gctcgtcgat cagttgtccg gccgcactct cggtctgcgc
     4141 gcggacatta cgccgcaggt cgcgcgtatc gatgcgcacc tgctgaaccg ccagggcgtg
     4201 acgcgtcttt gctatgccgg caatgtcgcg catacgcgtc cgcgcgggct gcatgcaacg
     4261 cgtgagcaga ttcagatcgg cgcggaaatt tacggccacg ccggtctcga agcggatctg
     4321 gaaatccagc agttgatgct cgatgcgttg cgtctcgctg gcctcgcgaa agtgcggctc
     4381 gatctgtgtc atgccggtgt gctcgccgcg ctgatcgaag cggagcctgc cgccgccgaa
     4441 ttgggtcaat cgctttacga cgcattggcc ggcaaggatg tgccacgcct tgtcgagttg
     4501 acggccgatc t
//
LOCUS       DATASH010000190         4528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2002881,
            whole genome shotgun sequence.
ACCESSION   DATASH010000190 DATASH010000000
VERSION     DATASH010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4528
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2002881"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="VHV99_05370"
     CDS             <1..704
                     /locus_tag="VHV99_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HEX3634907.1"
                     /translation="ISIRALARTAHDAQRHGNRGAQTWLSELIWRDFYFSVLHHFPYV
                     GVPGDHKAFKPEYDRIQWETGDAADANFAAWCEGQTGYPLVDAAMRQINQSGYMHNRL
                     RMVVASFLTKDLGLDWRRGEQYFESLLNDFELSSNNGGWQWASSSGCDAQPYFRIFNP
                     VTQSEKFDARGKFIRHYVPELAALSDKDIHAPWKAKPDALKAAKISLGAEYPMPVVDH
                     DTARKITLARYAVVK"
     gene            complement(737..2095)
                     /locus_tag="VHV99_05375"
     CDS             complement(737..2095)
                     /locus_tag="VHV99_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634908.1"
                     /translation="MTTQSGLSNTFQALPNPRGGAVRIAEMALTDALHAFMRDGDTLG
                     VLRRGIGALQLAGWLPAQRFAETLVLVSPLAGLSDKNRNQFAEALRDFSAAVERRNLR
                     ELACSTLLFEHYRGLHALMAAHTRAAPVSFDDLALAGRAIAPATLRAISSERLARVRA
                     HYERALLILLRSKAGELKGEAEIVSILDACLADLAGPDPYDFWRLAIGVIEALRLQPD
                     PDTAADARRLYARFNLVLADQAHGLSHAPKSLVRAALALLWRDYALYGASAEDSDQVD
                     VLRDYGLTVDWHIAASQASEDAWERNAEQARDASTRDLGVLRVNAVAYEDFLTGAEPA
                     IGALIEHGMSAREAGRIDVPAALKAADAAYRLGAAACALGLGHVALLADTLGLAWRRI
                     AYEAQTTPEGSPLDASIPEKAAQALGAMLHQAAAGIAPSEGGASVAALTAMIERGMAV
                     AR"
     gene            complement(2466..2636)
                     /locus_tag="VHV99_05380"
     CDS             complement(2466..2636)
                     /locus_tag="VHV99_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013436118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEX3634909.1"
                     /translation="MEYRSWMCLICGWIYDEEAGLPEEGIAPGTRWEDVPINWTCPEC
                     GARKEDFEMVQI"
     gene            3017..3898
                     /locus_tag="VHV99_05385"
     CDS             3017..3898
                     /locus_tag="VHV99_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HEX3634910.1"
                     /translation="MPSDTPPIVLTFGLSDPTGGSGLQADLLTLASMGCHGLTVLTGY
                     TVRDSATCDEVTGLDPDTVGTQARMLLEDMPVAAFKIGAATRAEVVSAIAEVVADYDD
                     VPLILAPDFTLDDEHVLAADELRESIADLLVPQTTMLVADHATLLQLAQPDGDAEAPT
                     LDAAISHLLSQGCEYILATETGTHRHVNTLYSEEGQIRQDTWDRTEHRMMGITDTLGS
                     AIAALLANGQEPAEAAREAQEYVFQATRAAFRPGMGAYLPDRFFWARSNDDDETPPVV
                     IDPQTDAPPVIPGEARH"
     gene            3923..>4528
                     /locus_tag="VHV99_05390"
     CDS             3923..>4528
                     /locus_tag="VHV99_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008342678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX3634911.1"
                     /translation="MPLRKPSLPPLKHSSGPVRIGCAGWGLTDAVAEHFPGEGTHLAR
                     YSQVLTCVEINSSFYRPHRAETYARWRDSVPGTFRFSVKIPKAITHDARLVDCEPLLD
                     AFLEAATQLGGTLGCWLVQLPPSLAFDPNVAKSFFTSMRERTDIPIALEARHESWFAK
                     GAATMLKSKEIAYVDADPQPDDCFIRYRADTSLVYFRLHGSP"
BASE COUNT          894 a         1481 c         1324 g          829 t
ORIGIN      
        1 ccatttccat tcgcgcactc gctcgcactg ctcacgacgc tcagcgccac ggcaatcggg
       61 gcgcgcaaac atggctgtcc gaattgatct ggcgcgactt ttatttctcg gtgctgcacc
      121 actttccgta cgttggcgtg ccgggcgatc acaaagcgtt caagccggaa tacgaccgca
      181 ttcaatggga aacgggcgat gccgccgatg ccaatttcgc cgcgtggtgc gaaggccaga
      241 cgggctatcc gttagtcgat gccgccatgc gccagatcaa tcagtcgggt tacatgcata
      301 accgcttgcg gatggtcgtg gcgagttttc tcaccaaaga cctcgggctt gactggcggc
      361 gcggggaaca gtatttcgag tcgctgctga atgacttcga gctgtcatcg aacaatggcg
      421 ggtggcagtg ggcgtcgtcg agcggttgcg acgcgcagcc gtatttccgc attttcaatc
      481 cggtcacaca gtcggagaaa ttcgatgcgc gcggcaagtt catccgtcat tacgtgccgg
      541 aactcgccgc gttgtcggat aaagacatcc atgcaccgtg gaaagcgaaa ccggacgctt
      601 tgaaggccgc gaagatttcg ctgggcgctg aatatccgat gccggtcgtc gatcacgaca
      661 cggcgcgcaa gatcacgctc gcgcggtatg ccgtggtgaa gtagcgcggg tttgtcactg
      721 aggcgcgata gaaaacctat cgcgcgacag ccatgcctct ttcgatcatt gcggtcagcg
      781 ccgccaccga cgccccgccc tccgaaggcg caatgcccgc tgcagcctga tgcaacattg
      841 cgccgagcgc ctgagcggcc ttctcgggaa tcgaggcatc gagcggcgag ccctccggcg
      901 tcgtctgagc ctcgtacgca atccgccgcc atgcgagccc aagcgtgtcg gcgagtaacg
      961 cgacatgacc cagccctaac gcgcacgccg ctgcgcccag ccgatacgcg gcgtcggctg
     1021 ccttgagtgc agccggcaca tcgatacggc cagcctcccg cgccgacatc ccatgctcga
     1081 tcaacgcgcc gatagccggc tccgcgcccg tcagaaagtc ttcgtacgcg acggcgttca
     1141 cacgtaatac gcccagatcc cgcgtggaag catcacgcgc ctgctccgca tttcgctccc
     1201 acgcgtcctc ggacgcttgc gacgcggcaa tgtgccaatc cacagtcaag ccgtaatcgc
     1261 gcaacacatc gacctggtca gaatcctccg ctgacgcgcc gtataacgcg taatcgcgcc
     1321 acagcagcgc gagcgcggcg cgcaccaggg atttgggtgc atgcgacaaa ccgtgcgcct
     1381 gatcggccag aaccagattg aaacgcgcgt acaggcgacg cgcgtctgcg gctgtgtccg
     1441 gatccggctg caaacgcaat gcttcgatta ccccaatcgc cagacgccag aaatcgtagg
     1501 gatccgggcc cgcgagatcg gccagacagg cgtcgaggat ggaaacgatt tccgcctccc
     1561 ccttcaactc gcctgccttc gaacgcagca ggatcagcaa cgcacgttcg tagtgcgccc
     1621 gcacacgcgc aaggcgctcc gaggaaatag cgcgcagtgt agccggcgca atggcgcggc
     1681 cggcgagcgc gaggtcatcg aaggaaacgg gtgcagcacg ggtatgcgcg gccatcagcg
     1741 catgcagccc gcgataatgc tcgaacagca gcgtggaaca ggcgagctcg cgcaggttgc
     1801 ggcgctccac cgccgcgctg aaatcacgca gcgcctcggc aaactggttg cgatttttgt
     1861 cgctcagccc ggccagcggt gacaccagca ccagggtctc ggcgaaacgc tgcgcaggca
     1921 gccagcccgc caactgcagc gcaccaatcc cgcggcgcag gacgccgagc gtgtcgccat
     1981 cgcgcatgaa tgcatgcagc gcgtcagtca acgccatctc cgcaatccgc acagccccgc
     2041 cgcgcggatt gggcaatgcc tggaacgtgt tgctcaaacc ggattgagtt gtcatggaac
     2101 gctcacgagc cgatgcgcgg tgctaagccc caagcatcgg atggatcttg atcgaacccg
     2161 ggtgcatttt cgccaatatg ggccgatgca tgccgctgca ttttcctctg gcgataaaac
     2221 ccggacacac gccggaaaag tcgagtcggg tcgagccaac tcactggccg agcgttcctg
     2281 ctcactgatc tatctttggt ctaaccgacc ggcatgcacg tcgcgaaaaa cgaaacgatc
     2341 tacgcaacga accaggaaac gtcgcgaacc tcacgcctgt cgccaacgcg taaacaaggc
     2401 gcgccgcatg aagatatccc atccatgcgg cgcggttttt ccttgcacga cgtccaagcc
     2461 tttgctcaga tctggaccat ttcgaagtct tccttgcgtg cgccgcattc gggacacgtc
     2521 cagttaattg gaacgtcttc ccagcgagtg cccggcgcaa ttccttcttc gggcaatccc
     2581 gcttcttcgt cgtaaatcca gccacaaatc aggcacatcc agcttctgta ttccatactt
     2641 atgtcgcgtc gggcaatcgg ttaaatgagt gcatgatggt accggtttgg cggcgcgatg
     2701 cctagcaatg cggcagcgct ggctgatcgg tcaggattcg gacggttttc ccggcttcgt
     2761 ctggagggtt cgtcggcttc gtcgaccccc gtcgccggaa ccgcccatgc aagcgctatg
     2821 cggcctgcga ggcccttcca gcaagtatcg gtccttcgtt gcaccggttt ccgcacaatg
     2881 attcatccaa ctttggcgcg aaaaaaaggc catctcatgt gccggcgtga ctttaggccg
     2941 cataatcgtg ccgtttgagc cgactcattg tccggccgct tgaatagccg attagattga
     3001 acccacgaat aatccgatgc ccagcgacac ccctcctatt gttctcacct ttggcctgtc
     3061 cgatcccacg ggcggctccg gcctgcaggc agacctgctg acccttgcca gcatgggctg
     3121 tcacggactc actgtcctga ccggctacac cgtgcgcgac tcggcgacct gcgacgaagt
     3181 caccgggctc gatcccgaca ccgtcggcac tcaggcccgc atgttgctgg aagatatgcc
     3241 ggtggcggcc ttcaagatcg gcgcggcaac gcgtgcggaa gtggtcagcg caattgcaga
     3301 agtggtcgcc gattacgacg acgtgccgct gatcctcgcg cccgacttca cgctcgacga
     3361 cgaacatgtg ctggcggcgg acgaattgcg cgaatcgatt gcagatttgc tggtgccgca
     3421 aaccaccatg ctcgtggccg atcacgccac cctcctgcaa ctcgcgcagc ccgatggcga
     3481 cgccgaagcg cccacgctcg acgccgcgat ctcgcatttg ctgtcgcaag gctgcgagta
     3541 catcctggcg acggaaacgg gcacgcatcg gcatgtgaac acgctctaca gtgaggaagg
     3601 ccagatccgg caggacacgt gggaccgcac cgagcaccgc atgatgggca ttaccgacac
     3661 gctcggatcc gccatcgccg cgttgctcgc caacgggcag gagcccgcgg aagccgcgcg
     3721 cgaagctcag gaatacgtgt tccaggcgac ccgcgcagcg ttcaggcccg gcatgggcgc
     3781 gtatttgccg gaccggtttt tctgggcgcg ttcgaacgat gacgacgaga caccgcccgt
     3841 ggtgatagat ccacagacag atgcaccacc cgttataccg ggcgaagccc gccactgata
     3901 tttcctggag cgagactgac acatgccgct gcgcaaacca tcgctccccc cactcaagca
     3961 cagttccggc cctgtacgaa tcggctgcgc cggctggggt ctcacggacg cggtcgcgga
     4021 acatttcccc ggcgagggta ctcatctcgc ccgctattcg caggtgctga cctgtgtcga
     4081 aatcaactcc agcttttacc ggccgcatcg ggcggaaacc tatgcccgct ggcgcgacag
     4141 cgtgcccggc acgtttcgct tttcggtgaa gatcccgaag gccattacac acgacgcacg
     4201 cctcgttgat tgcgagccgc tgctcgacgc tttcctcgaa gccgcgactc aactgggcgg
     4261 tacgcttggc tgctggctcg tgcaactacc gccgagcctg gcgttcgatc ccaatgtggc
     4321 caagtcgttt tttacatcga tgcgcgaacg cacggacatc ccgatcgcgc tggaagcacg
     4381 ccacgagagc tggttcgcga aaggcgcggc cacgatgctc aagtcgaaag agatcgccta
     4441 cgtcgatgcc gatccacagc ccgatgattg tttcatcagg tatcgggcgg atacgtcgct
     4501 ggtgtatttc cggctgcacg gctcgccg
//
LOCUS       DATASH010000191         2727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5563973,
            whole genome shotgun sequence.
ACCESSION   DATASH010000191 DATASH010000000
VERSION     DATASH010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5563973"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VHV99_05395"
     CDS             <1..455
                     /locus_tag="VHV99_05395"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEX3634912.1"
                     /translation="HSHFGVGHQPGLSDVLSGGDLTAMIQRDVLPGLDVLAKGTLPSH
                     PSELLMSKRFETMLEELKSHYDLVIVDTPPVLAVTDSTVIGKYAGTTLLVVRHGRHPL
                     NEIAETAKRLLNGGVSLRGVLLTDVPQEGAFLGSGYQGGYYGYDSIAG"
     gene            547..1665
                     /gene="gmd"
                     /locus_tag="VHV99_05400"
     CDS             547..1665
                     /gene="gmd"
                     /locus_tag="VHV99_05400"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX3634913.1"
                     /translation="MKRKVALITGITGQDGSYLAELLLAKDYDVHGIKRRSSLFNTDR
                     IDHLYRDPHDPDQRLFLHHADLTDSTSILRVIQRVEPDEIYNLAAQSHVAVSFEEPEY
                     TANADGLGALRILEAIRILGLQHKTRFYQASTSELYGLVQQIPQSETTPFYPRSPYAV
                     AKLFAYWTTVNYREAYGLYACNGILFNHESPVRGETFVTRKITRAVARIAVGMQKILY
                     LGNLSALRDWGHARDYVEMQWRMLQQEQPEDYVIATGVQYSVRDFVRHAAAELGVTVR
                     FEGTGVDEIGIVEKVEGREIKMSPGDVIVRVDPRYFRPAEVETLLGDPSKAHAKLGWQ
                     PTTSFASLVKEMVRADYQIARRDALVTLAGFTALEHHE"
     gene            1671..2630
                     /locus_tag="VHV99_05405"
     CDS             1671..2630
                     /locus_tag="VHV99_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HEX3634914.1"
                     /translation="MNKQARIFVAGHRGMVGSALVRRLEADGYHNVVTRSRAELDLMD
                     QAAVNGFFEREKIEVVLLAAARVGGILANASQPGEFIYENLVIETNVIHAAFRAEVER
                     LVFFGSSCIYPKQCPQPIREEYLMTSPLEPTNDAYAIAKIAGLKLCEAYNREYNTQYV
                     SLMPTNLYGPNDNYDLNSSHVLPALLRKAHEAKVNGSAALTVWGSGTPRREFLHVDDL
                     AAATLFVLEHNVTEGLFNVGVGEDLSIRELAECICKVVGFEGELVFDASKPDGTPRKL
                     LDVSRLAQLGWHATIGLEEGLASTYREFVELHAGSTSVAALHV"
BASE COUNT          519 a          826 c          865 g          517 t
ORIGIN      
        1 ttcattcgca ttttggcgtg ggccatcaac cgggactctc cgacgtgctg agcggcggcg
       61 atctgacggc gatgatccag cgcgacgtgt tgcccggact cgatgtgctc gccaagggca
      121 ccctgccttc gcatccgtct gaattgctga tgagcaagcg cttcgaaacg atgctcgagg
      181 aattgaagtc gcactacgac ctcgtgatcg tcgacacgcc gccggtactc gccgtgaccg
      241 actcgacggt gatcggcaag tacgccggca cgacgctgct ggtggtgcgc catggccgcc
      301 atccgctgaa cgagattgcc gagacggcca agcggctgct caacggcggc gtctcgctca
      361 gaggcgtgct gctgaccgac gtgcctcagg aaggggcttt cctcggttcc ggctatcagg
      421 gcggctatta cgggtacgac agcattgccg gctaagggtt gacgcagcgg cggctccacg
      481 acgggccgct cgctcgaatc gtgctgttgc attggcatcg caaggtttaa cgagagggat
      541 agctccatga aacgcaaggt cgcgctgatc accggcatca ctggacagga cgggtcgtat
      601 ctcgccgagc tgctgctcgc caaggattac gacgtacacg gcatcaaacg caggtcatcc
      661 ctgttcaata cagaccggat cgatcatctc tatcgcgatc cgcacgaccc ggatcagcga
      721 ctcttcctgc atcacgccga cctgaccgac tcgaccagca tcctgcgcgt gatccagcgg
      781 gtcgagcctg acgagatcta caacctcgcg gcgcagagcc atgtggcggt ttcgttcgag
      841 gaaccggaat acacggccaa tgccgacggc ctcggcgcgc tgcggattct cgaagcgatc
      901 cggattctcg ggcttcagca caagacgcgt ttctatcagg cttcgacctc ggagctgtac
      961 ggcctcgtgc aacagattcc gcaatcggag accacgccgt tctatccgcg cagcccgtac
     1021 gcggtggcca agctgttcgc gtactggacc acggtcaatt accgcgaggc ctacgggctc
     1081 tatgcctgca acgggattct gttcaatcac gaatcgccgg tgcgcggcga gactttcgtg
     1141 acgcgcaaga tcacgcgtgc cgtggcacgc atcgcggtgg gcatgcagaa gatcctgtat
     1201 ctcggcaatc tgtcggcgtt gcgcgactgg ggtcatgcgc gcgactacgt ggaaatgcag
     1261 tggcgcatgc tgcaacagga acagccggag gattacgtca tcgccaccgg cgtgcagtac
     1321 agcgtgcgtg acttcgtccg gcatgccgcg gccgaactgg gcgtcaccgt gcgtttcgaa
     1381 ggcaccggcg tggatgaaat cggcattgtc gagaaggtgg aagggcgcga gatcaagatg
     1441 tcgcccggcg acgtgatcgt gcgtgtcgat ccgcgctact tccgtcccgc cgaagtcgag
     1501 acgctgctgg gcgatccgtc gaaagcgcat gccaagctcg gctggcagcc gactacgtcg
     1561 tttgcctcgc tcgtcaagga aatggtgcgc gcggactatc agattgcaag acgtgacgcg
     1621 cttgtcacgc tggccggttt cacggcgctg gaacatcacg agtaacggca atgaataaac
     1681 aagcacgcat cttcgtcgcg ggccaccgcg gcatggtcgg ctccgcgctg gtccggcggc
     1741 tcgaggccga cggttatcac aacgtcgtca cgcgttcgcg ggcggagctc gatctcatgg
     1801 atcaggctgc ggtgaatggc tttttcgagc gggagaagat cgaggtggtg ctgctcgcgg
     1861 ccgcgcgggt gggcggcata ctcgccaatg cgtcgcagcc gggtgagttc atctatgaaa
     1921 acctggtgat cgaaaccaac gtgattcacg ccgcgtttcg tgctgaggtc gagcggctgg
     1981 tgttcttcgg ttcgtcatgc atttatccga agcagtgccc gcagccgatc cgcgaggagt
     2041 atctgatgac ctcgccgctc gaaccgacca acgacgccta cgcgatcgcg aagatcgccg
     2101 gcctcaagct gtgcgaggca tacaaccgcg aatacaacac gcagtacgtc tcgttgatgc
     2161 cgaccaatct gtacggcccg aacgataact acgatctgaa cagcagccat gttttaccgg
     2221 cgctgctgcg taaggcgcac gaagcgaagg tgaacggttc cgcggccttg acggtgtggg
     2281 gctcgggcac gccgcgccgc gagtttctgc acgtcgacga tctcgccgcg gcgaccctct
     2341 tcgtgctcga gcataacgtg acagaaggcc tgttcaacgt cggcgtgggt gaagacctgt
     2401 cgattcgcga actggccgag tgcatctgca aagtggttgg cttcgagggc gaactcgtct
     2461 tcgacgcctc caaacctgac ggcacgccgc gcaagctgct cgacgtatcg cggctcgcgc
     2521 aattgggctg gcacgcgacc attggccttg aggaaggcct cgcgtccacg tatcgggaat
     2581 tcgttgaact acacgccggc tcgacctccg tcgccgcctt gcacgtatag gcgcgcactg
     2641 aagggccgtt gagcgcgatt ccggaattgg gggaacgcgt ggcgcgtgac gcgcgagtag
     2701 cgtgcaacgc acacagggac gattttt
//
LOCUS       DATASH010000192         3896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5366518,
            whole genome shotgun sequence.
ACCESSION   DATASH010000192 DATASH010000000
VERSION     DATASH010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3896
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5366518"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /gene="cbiE"
                     /locus_tag="VHV99_05410"
     CDS             complement(<1..749)
                     /gene="cbiE"
                     /locus_tag="VHV99_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008353119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6y C5,15-methyltransferase
                     (decarboxylating) subunit CbiE"
                     /protein_id="HEX3634915.1"
                     /translation="MSPWLTVVGIGEDGWRGLSRHARQALLAADTIFGGARHLALLPA
                     RIGGERHAWPTPFTIAPVLEYRCERKRVCVLASGDPMMFGVGSTFARELAADEFCVMP
                     APSSLSLAAARLHWGLQDVAVVSLVGRPVTALETQLHHGARLFVLSSNAASPGVVAGL
                     LTKRGFGPSRLSVFEHLGGERERRIDSVASAWTASEVAALNLVAIECIAENHARRLPL
                     TPGLPDDAYRHDGQLTKRDVRAITLSRLAPEP"
     regulatory      893..1070
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1264..2505
                     /gene="cobG"
                     /locus_tag="VHV99_05415"
     CDS             1264..2505
                     /gene="cobG"
                     /locus_tag="VHV99_05415"
                     /EC_number="1.14.13.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008353118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0019250 - aerobic cobalamin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B synthase"
                     /protein_id="HEX3634916.1"
                     /translation="MRIVPARDGGLCRIRLAWGELSAAQARVVAQVSLEFGSGFIDAT
                     NRANLQIRGVTEESDAQDALVSRLVDAGLGPDTPGSDDQRNVMSSPLAGLDSHALLDV
                     VPLVSTLIGQMQRDARLHQLSPKFALMVDGGERLAMLEHPHDVWLSAISAERFVFGLA
                     GCPPRFENDAPALAAVKAGHVPELVDALLHTFVDLAQPWQTRMRDLLTTYSIEDILGR
                     VQMKLGSALDRDVEHWRRPASDARLRFGVHRQATGDSVCVGAQVPLGRLDAATLYALA
                     DLAPRLRMTPWQSVMLLDVPSANAQAVVEQLTALGLATNPDEPFARLIACTGSQGCAK
                     SFANTKADAHLLARSMPPAGDVHLSGCARSCAAAHPVETTLMAVSPGRYDLYGNAPEP
                     LARHLTIEQAARWLARSHLDA"
     gene            2498..3124
                     /locus_tag="VHV99_05420"
     CDS             2498..3124
                     /locus_tag="VHV99_05420"
                     /EC_number="5.4.99.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017778279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016993 - precorrin-8X methylmutase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HEX3634917.1"
                     /translation="MLDYIRDGQAIYRASFATIRAEANLSAVPCDLEKLAVRVIHACG
                     MTDVIDDLVFSPGAGTAGRLALSRGAPILCDARMVAEGITRARLPADNRVICTLNDPS
                     VPERARTLGNTRSAAALGLWRPDLEGSVVVIGNAPTALFHLLDMLDAGAPKPALILGF
                     PVGFIGAAESKALLAADSRGVPFVALRGRRGGSAMAAAAVNALATETE"
     gene            3125..3850
                     /locus_tag="VHV99_05425"
     CDS             3125..3850
                     /locus_tag="VHV99_05425"
                     /EC_number="2.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008353116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030788 - precorrin-2
                     C20-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-2 C(20)-methyltransferase"
                     /protein_id="HEX3634918.1"
                     /translation="MSGRLYGLGVGPGDPELITVKALRMLTSAQVVAYFVAKGKKGNA
                     FGIIEGHLKETQTRMPLVYPVTTEALEPPLCYETIIGDFYDESALRIADHLEAGRDVA
                     VICEGDPFFYGSYMYLHDRLAGRFEAHVVPGVCSMLGGVAVLGVPLVYRNQSLSVLSG
                     VLPEADLRQRLATADAAVIMKLGRNFDKVRRVLTELGLDKRALYVERATMANQRIVPL
                     EEVDPMASPYFSLLVVPGEKWQA"
BASE COUNT          709 a         1268 c         1197 g          722 t
ORIGIN      
        1 ggctcgggcg caagccgcga cagcgtgatc gcgcggacat cgcgcttcgt gagctgcccg
       61 tcatgccggt aggcgtcgtc tggaagaccg ggcgtcagcg gcaagcgccg tgcatggttt
      121 tccgcgatgc actcgatcgc caccagattc agcgcggcca cttcgctcgc cgtccacgcg
      181 ctggccacgc tgtcgatacg acgctcgcgc tcgcccccca gatgctcgaa tacactcagt
      241 cggcttgggc cgaaaccgcg cttggtcagc aaaccagcaa ccacgcctgg gctcgccgcg
      301 ttgctgctca gaacgaacag gcgcgcgccg tgatgaagct gcgtttcgag cgccgtgacc
      361 ggccgcccga ccagtgacac taccgcgacg tcctgaagcc cccaatgcaa acgcgcggcg
      421 gcaagtgaca acgaagacgg cgcgggcatc acgcagaatt catcagcggc cagttcgcgg
      481 gcgaatgtcg agccaacgcc aaacatcatc gggtcgccgc tcgcgagaac gcacacccgt
      541 ttgcgttcgc accgatattc gagcacgggc gcgatcgtga acggcgtcgg ccatgcatga
      601 cgttcgccgc caattcgggc gggcaacaac gcgagatggc gcgcgccgcc gaaaatagta
      661 tcggccgcga gcagcgcctg gcgggcgtgc cgactcagtc cgcgccagcc atcctccccg
      721 attccgacca cggtcaacca cggtgacatc cgccgatcct cgcttgattt attcgtcaac
      781 tgattcgcca actcattcac tgattgcgcc tcgtctacaa tgcgcccggg acaatgcggc
      841 taaagcacgt ccggcctgac gccgatgcgc tttagccgct gcaaaaacgg gcataatagc
      901 gcggttagtc ggtgccctta cgggcctaac agggaacaca gcagaactgt ggctgccccc
      961 gcaattgtac gcagcgagtc cgcgctccac tgccactggt ttcgactggg aaggccggta
     1021 cggatcacga cctgcaagcc aagagacctg ccgaccgcaa ttcttcgtgc aagccaacat
     1081 cgggcggggt gtaccgatgc cgcgaacgtg ccatcccatg aaagggtccg gcatgctcga
     1141 ggcgccgcgc gagaccgaac gggtcacgct ttgaacgaac cactcactac tgtacgcgga
     1201 ttagcgcacg cacttacgga cgcatcttcg caagcatcgc ggccatcggc gtgtccgggc
     1261 ctgatgcgaa tcgtgcccgc gcgtgacggc ggcctgtgcc gcattcgcct cgcatggggc
     1321 gaactgagcg cagcgcaggc gcgtgttgtg gcgcaggtgt ccctcgaatt cggttccggt
     1381 ttcatcgatg caaccaatcg cgcgaatctc cagattcgcg gcgtaacaga agagagcgac
     1441 gcgcaggatg cactcgtctc gcggctcgtc gacgcgggac tcggtcctga cacgcccggt
     1501 tcggacgacc agcgtaacgt catgtccagt ccgctggcgg gcctcgattc gcacgccttg
     1561 ctcgatgtcg tcccgcttgt ttccactttg atcgggcaga tgcagcgcga cgcacgtttg
     1621 catcagctat cgcctaaatt tgcgttgatg gtcgacggcg gcgagcgcct cgcgatgctc
     1681 gaacatcctc acgatgtgtg gctttcggca atatcggcgg aacggtttgt attcgggctc
     1741 gcgggctgtc caccccggtt tgaaaacgat gcgcctgcgt tggctgcggt aaaagccggg
     1801 cacgtccccg agctggttga cgcgctgctg cacaccttcg tcgatctcgc gcagccctgg
     1861 cagaccagga tgcgcgatct gctgacgacg tattccatcg aagacattct tggccgcgtt
     1921 cagatgaagc ttggttccgc tttggatcgt gacgttgaac actggcgacg tcccgcatcc
     1981 gatgcaaggc tgcgtttcgg cgtgcatcgt caagcaacgg gcgacagcgt gtgcgtcggt
     2041 gcgcaagtgc cgcttgggcg cctggatgcc gcgacactgt acgcgctcgc cgatctcgcg
     2101 ccacggttac gcatgacgcc ctggcaaagc gtgatgctac tcgacgtgcc atccgccaat
     2161 gcgcaagcgg tcgttgaaca actcaccgca ctcggcctcg cgacgaaccc cgacgaaccg
     2221 ttcgcacgcc tgattgcatg tacgggttcg caaggctgtg cgaaaagttt cgccaatacc
     2281 aaagccgacg cacaccttct cgcccggtcg atgccgccag ccggcgacgt tcacctgagc
     2341 ggctgcgcgc gctcatgcgc ggctgcgcac cctgtcgaga ccaccttgat ggcagtttca
     2401 cccggccgct acgatcttta cggcaacgca cctgaacccc ttgcccgtca tctcaccata
     2461 gaacaggcag cccgctggct agcccggagc caccttgatg cttgactaca ttcgcgacgg
     2521 ccaggcgatt tatcgcgcgt cgtttgcgac cattcgcgcc gaagccaacc tctcggccgt
     2581 tccctgcgat ctcgagaaac ttgccgtgcg cgtgatccat gcatgcggca tgacggacgt
     2641 catcgatgac cttgtgttct cacccggcgc gggcacggca ggccgcctcg cgcttagccg
     2701 aggcgccccc attctctgcg atgcacgcat ggtcgccgag ggcatcacgc gtgcaaggct
     2761 gccggcggat aaccgcgtga tctgcacgct caacgatcct tccgtgcccg aacgcgctcg
     2821 cacgctcggc aacacgcgct ccgcggctgc acttgggctg tggcggccgg atcttgaagg
     2881 cagcgtagtg gtgatcggca atgcgcccac ggcgctcttc catctgctcg acatgctcga
     2941 cgcgggcgcg cccaaacctg cgctgatcct gggctttccg gtgggtttta tcggcgcggc
     3001 ggaatcgaag gcgctgcttg ccgccgatag ccgcggcgtg ccgttcgtcg cattgcgcgg
     3061 cagacgcggc ggcagcgcaa tggcggccgc tgccgtgaac gcgctggcaa cggagaccga
     3121 atagatgagc ggacgcctct atggcctcgg tgtcggtcct ggtgaccccg agctgataac
     3181 agtaaaggca ctgcgtatgc tgacctccgc gcaggtagtc gcttacttcg tggcaaaggg
     3241 caagaaaggc aatgcgttcg ggatcattga aggccatctc aaggagaccc agacgcgcat
     3301 gccgctcgtg tatccggtga caactgaagc gctcgagccg ccgctttgct acgaaacgat
     3361 catcggcgac ttctacgatg aatccgccct ccgcattgcg gatcatctgg aagcgggccg
     3421 cgatgtggca gtcatctgcg aaggcgatcc gttcttctac ggttcgtaca tgtatctgca
     3481 tgaccggctg gccggacgat tcgaagcgca cgtcgtgccc ggcgtgtgtt ccatgctggg
     3541 cggtgttgcc gtgcttggcg tgccgcttgt gtatcggaac cagagtttgt cggtgttatc
     3601 gggcgtgttg ccggaagcag atctcaggca acgactcgcc accgccgatg cagccgtgat
     3661 catgaaactg ggtcgcaact tcgataaagt gcggcgcgtg ctcaccgaac tcggactcga
     3721 taagcgtgcc ctttacgtcg aacgcgcgac catggcgaac cagcgcatcg tgccgctgga
     3781 agaagtcgat cccatggcgt cgccctactt ctcgctgctc gtcgtgccgg gggaaaaatg
     3841 gcaggcgtga acgaagggac acccatcgca atcgtggtgc tgggtacggg ggcgat
//
LOCUS       DATASH010000193        10733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3387607,
            whole genome shotgun sequence.
ACCESSION   DATASH010000193 DATASH010000000
VERSION     DATASH010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 10733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10733
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3387607"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..772)
                     /locus_tag="VHV99_05430"
     CDS             complement(128..772)
                     /locus_tag="VHV99_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3634919.1"
                     /translation="MTQKIRVIVADDHDCVRVGVMRLLQAAPHIEIVGEAPDTRTLAE
                     LLDTHACDVVVSDIGMPGIDGASNVVSFLRRLFRARPHPCVVILTMICHAHMLSGLLH
                     LGVAAIVDKRDTAAALIEAIEAAVAGGVYLSDQARVAIDTSDAPPQPRAGVLSAREWE
                     VFQLYVRGLAVHEIAARLQRSGKTISTQKRSAMRKLGLETENDLIYYARQIGLA"
     gene            complement(884..1576)
                     /locus_tag="VHV99_05435"
     CDS             complement(884..1576)
                     /locus_tag="VHV99_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3634920.1"
                     /translation="MNDNADLSTPVRTIIADDHPLVLLAIENLMAGYPNMQIVGRAAD
                     ATELFKEVDRIPCDLVLMDLYMPGGFYGDGLETIRRFKTRYPHIALVVLTMETEAAAL
                     KKVIALGVDGLISKRDRIDLIHVAVVTALARECYLGPAVRSLITEATVAQRLDFVRNM
                     LSRRELEVFTQYASGLGVTEIAARLGRSVKTISAQKCTAMRKLALHSDAELFRFAVEH
                     GVISEDCVQNRR"
     gene            complement(1727..2380)
                     /locus_tag="VHV99_05440"
     CDS             complement(1727..2380)
                     /locus_tag="VHV99_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3634921.1"
                     /translation="MNLRVILADDHPFVLLGVRAALEMHAGVTIVGEAATPTSLIQVL
                     QSTPCDVLVTDLTMPEASGAVEDGLDLVRRIRCSWPLLRIVVMTALTNTAILRAIVSD
                     GAISTLSKMETMDELWQAIEASGKGEIYVGRSIIEALAQPQDEACDPPPVSRLSRRQV
                     EVVRMSVRGQSIAEIAATLGCHRRTVSRQKREAMAKLGVTNDPGLFAYVRAYGVCES"
     gene            complement(2745..4061)
                     /locus_tag="VHV99_05445"
     CDS             complement(2745..4061)
                     /locus_tag="VHV99_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3634922.1"
                     /translation="MGNESIAAGPAPGRRAANHRWKVLGVGFAANASFSAAFSGIPTT
                     AVFLRSGYHLGNDGLGLVLGMLGLGIAVSELPWGLLTDRWGDRRVLLLGLLSTTAALA
                     GLALFVVPSGTHVPNVATLAFGLLLVGLLGGSVNGSSGRAVMGWFREGERGLAMSIRQ
                     TAVPAGGGLGALILPVLASRFGFASVYTVLALACAITAGFAWCWLHEPSHAEGSDVGP
                     ARHAAAAAAVPAKVSPLRDMGIWRVALGAGALCVPQIAVVTFGTVFLHDFGAAGVLAI
                     SVTMAAVQTGAAIARVWSGTWTDRRGNRRAYMRACSVLTAVLFALLALVTGFAGMHHA
                     ALTAVFALMIVLGGISGSAWHGVAFTELATLAGTSRAGTALAMGNTCVFLTLFLTPLA
                     IPPLLSVGSWPMVWAVASVCALIALPVLPRAARASKLTAARACDAA"
     gene            4160..5047
                     /locus_tag="VHV99_05450"
     CDS             4160..5047
                     /locus_tag="VHV99_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015001573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3634923.1"
                     /translation="MSQRGFDLTQLRTFVAVAESGSVSAGAERVFLSQSSVSEQLKKL
                     EERAGQPLFVRSKQGVSATPAGSRLLDHARRIIAMSEAAFEDLQGRSLDGELRIAITD
                     YYRPLDIARILKTFSEQHPRLKLHVTVLPSAVIDSSAGDDASYDIGLSLRLVTGNPRA
                     PGRRAGAQSTVVRRERLLWVCAADASPRPATPYPLVLLPSRCQLQRFVVKLLDEHKVP
                     YLVSHSASGVAGLQLALKAGLGISCLNESSIGGGVVACPPSVGLPALPAVEFHLLPGR
                     VGESERVTNARTALTRLFS"
     gene            5541..6257
                     /locus_tag="VHV99_05455"
     CDS             5541..6257
                     /locus_tag="VHV99_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX3634924.1"
                     /translation="MHRRTIGFSILLMFYVVWRGAAFAATDASGAMPSAMDERVRACT
                     SCHGVQGQGLNNDYFPRIAGKPADYLFNQLSAFRDGGRTYPPMGYLLAFLPDDYLRKI
                     SQYFADLQPPYPNADHSDVAPAVLAAGQRLVTQGDPARKIPACIACHGARMTGTQPGI
                     PGLLGLHASYIAGQMGTWRSGTRHALAPDCMHSIAVKLTDKDITAVSNWLARQPRPAD
                     PRPAPASAERLPLACGSQPQ"
     gene            6296..7654
                     /locus_tag="VHV99_05460"
     CDS             6296..7654
                     /locus_tag="VHV99_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX3634925.1"
                     /translation="MTKRRRTAASLVSGLLVVGLIVAGSAALPGAARALLITEARAAE
                     AVPAGTTTAPAKTAAATGDKTDTRPDIVKRGEYLARAGDCVACHTAPRGKLFAGGLAM
                     DTPFGTLYSPNITPDAQYGIGTWSEDAFFKMMRTGRTPDGTLLYPAMPIAQYTKVTRE
                     DSDAIFAYLKSVTPVREPNHKHTLRFPFNQRKLLYGWRTLYFREGEFQPDPTKSVEWN
                     RGAYLVDGLGHCTMCHTKINMLGGSSQSEQFAGGLIPVQNWYAPSLTSDKDGGLCDWS
                     IKDIVDLLQAGISDRGAVYGPMAEVTYHSLQYMTEADVKAMAVYLKTLPDNRGRKSGP
                     SAPTNTNVFALGEKIYADKCALCHGAKGEGQLQHYPPLAGNQSIEMDSAVNPIRIVLN
                     GGFPPGTMRNPEPYGMPPFGQELNDTDAAAVVTYIRTAWGNHGQPVTSREVNELRKAP
                     LH"
     gene            7685..7861
                     /locus_tag="VHV99_05465"
     CDS             7685..7861
                     /locus_tag="VHV99_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634926.1"
                     /translation="MEANDTRSAAPPTDEEVERVVAAGPHGAIAVAGIATLIVMAIWF
                     GFYFLVFLPRGVIH"
     gene            7864..8436
                     /locus_tag="VHV99_05470"
     CDS             7864..8436
                     /locus_tag="VHV99_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit II"
                     /protein_id="HEX3634927.1"
                     /translation="MSTDSNHQPGSGHQVALRAERRWAIFATALIVLMLAVIVFSGLH
                     WAMMPPSRVETIDPSRLQLSGEFVEDNLGSAVEPDGSVVVRFIAQQYSFTPQCLLVPA
                     DTPITIRTTSADVVHGLLVTDTNINTMVVPGYVATLNTRFDKPADHTMPCHEFCGFGH
                     QTMWAHVKVIDKATFFEQARQSRRLSCVSR"
     gene            8423..10048
                     /locus_tag="VHV99_05475"
     CDS             8423..10048
                     /locus_tag="VHV99_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="b(o/a)3-type cytochrome-c oxidase subunit 1"
                     /protein_id="HEX3634928.1"
                     /translation="MFHAKRLVLAHFWLAFIAFLIALLLGAWQMLVRSPLVPWVGDPE
                     LYYRSVTAHGSVMAYVLPTLVSMGFGYAIVELALAQRLVGLKWAWAAFIMLAVGAVMA
                     MVPVALGKASVLYTFYPPMIGSPFYYLGVVLVVVGSWIWVALMHVNLRVWKRANPGKP
                     IPLAMFANVAGAYLWAWTAIGAALEILFQILPVAFGLTNTIDAGLSRILFSWTLHAIV
                     YFWLIPAYIAYYTLVPRAIGGRLYSDSMARVSFILFLVFAMPIGIHHLFADPQVGSGF
                     KFLHAVFTGMVSVPTLLTVFTICASVEIAGRLRGGKGAFGWLTALPWQNPMMLVIAFS
                     FVMLGLGGAGGLINMSYQLNSTIHNTQWVTGHFHLIFGGAIVIMYFAIAYELWPQLTG
                     RAIASVKLVRTQLWLWFIGMMVVTLPWHYVGLLGAPRRMAYYDYNTPGMESQAFWVGM
                     SAVGGLILVVSGVLFIYILAKSQFGPEVQQQSFRFASAVQPVEHVPAALNSFGLWVAL
                     MIGLTVVNYSVPIVQLLRLPQTSVPAVVVGAQR"
     gene            10045..>10733
                     /locus_tag="VHV99_05480"
     CDS             10045..>10733
                     /locus_tag="VHV99_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX3634929.1"
                     /translation="MSQERVFSLRNPWFTVSLGGTIAIAVVAMLIGFIWLPSANSSFS
                     ERGLWATICSAAGVPSSWYGGGSNIAGPAPSEVVVLPPLRRARADANSIGRGATIATQ
                     NCSMCHGVLGTVQVTAPALAGQYADVVYKELRDYQNGVRQNAIMQPIIAARSDQDLHD
                     LAAYYASLPRAPAAEVLPTGARPDANAVKLAMQGDPQRNIAPCAACHGQLDRKGAAPW
                     LGGQSSIYLAA"
BASE COUNT         2043 a         3363 c         3225 g         2102 t
ORIGIN      
        1 gcgggtgttg gaagtcgagc ctcagctaat tcagaactat gaaacgggaa tgtccctcaa
       61 ccaatcagaa gactcctaaa gtcctaagaa ctgtcggctg tcgaggcctg gttctctcac
      121 acgactgtca ggccaaccct atctgtctcg cgtagtagat gaggtcgttt tctgtctcga
      181 gaccgagttt gcgcatcgcg ctacgcttct gcgtgctgat ggtcttgccg ctgcgttgca
      241 gacgcgcagc gatttcgtgg actgccaggc ctcgcacata gagctggaag acctcccatt
      301 cgcgcgcgct caacacgccc gcgcgcggct gcggcggcgc atcggaggtg tctatagcga
      361 cgcgtgcctg atccgataga taaacaccgc cggcaaccgc agcctcgatc gcttcgatca
      421 acgccgcggc ggtatcgcgt ttgtcgacga ttgcggccac accaaggtgc aacaatccag
      481 aaagcatgtg cgcatggcag atcatcgtca ggatgacgac acaaggatgc ggccgagccc
      541 ggaacaggcg gcgcaggaaa gacacgacgt tgctggctcc gtcaatgccc ggcatgccga
      601 tatccgacac gacgacgtcg cacgcatgtg tgtcgagcag ttcggcaagc gttcgggtat
      661 cgggcgcctc accgactatc tcgatgtggg gtgccgcttg cagcaggcgc atcacgccaa
      721 cgcggacgca gtcgtggtcg tcggccacga tgacgcggat cttttgtgtc atttttttgt
      781 tgtcttattg actggcactc cccgcacgcg acgtgcatcc cctggggtgc caagtgcctc
      841 ttccattcac cacgccaccg gaccggcgcg acgtcttatc ggctcagcgc cggttttgca
      901 cgcaatcttc ggagatcacg ccgtgctcga cggcgaagcg gaacagttcc gcgtcgctgt
      961 gcaaggcgag cttgcgcatc gcggtacatt tctgcgcact gattgtcttg acactgcgcc
     1021 caaggcgcgc ggcgatctcg gttacaccca gcccggaagc gtattgcgtg aacacctcca
     1081 attcgcggcg tgacaacatg ttccggacaa aatcgagccg ctgcgcgacc gttgcttcgg
     1141 taatcagcga tcgcaccgcc ggccccaggt agcattcacg tgccagtgcc gtgacgactg
     1201 ccacatgaat cagatcgatc cggtcacgct tgctgatcag cccatcgaca cctagcgcga
     1261 tgaccttttt cagcgcagca gcttcggttt ccatggtgag cacgacaagt gcgatgtggg
     1321 gatagcgtgt cttgaaccgc ctgatggttt ccagtccgtc gccgtagaaa cctcccggca
     1381 tgtagaggtc catgagaacg aggtcgcatg gaatacgatc gacttccttg aaaagctctg
     1441 tggcgtcggc agcgcggccg acgatttgca tattcggata gcccgccatc agattctcga
     1501 tcgccaacag aaccaatgga tgatcgtccg cgatgatggt tcgaactggt gtgcttaagt
     1561 cggcgttgtc gttcatcgtc tttttctccc ccgtacctga attacccgtc gctctctccg
     1621 gagcaactgg ccctgtcgcg atcattaaac atatttcgga gaatagacat aagatcgctc
     1681 tgaaatagct caatgagctc atgcagataa tgattagcta tacgatctaa ctttcacaaa
     1741 ctccatatgc acggacataa gcaaagagac cgggatcgtt ggtaacgcca agtttcgcca
     1801 tcgcttcgcg cttttgacgg ctgacggttc ggcgatgaca tccgagcgtt gccgcgatct
     1861 ccgcgatcga ttgaccacgg acggacattc tgacgacttc cacctgtctg cgggacaaac
     1921 gtgaaaccgg tggcggatcg cacgcttcgt cctgaggctg ggcgagcgct tcgatgatgg
     1981 aacggccaac gtaaatctcg ccctttccac tcgcctcgat cgcttgccac agttcgtcca
     2041 tggtttccat tttgctgagc gtactgatcg caccatccga cacgatggca cgcagaatcg
     2101 ccgtgttcgt cagagccgtc atcacgacga tgcgcaacaa tggccacgaa caccggattc
     2161 gcctgacaag gtccaggcca tcttctaccg cacccgatgc ctccggcatc gtcaggtcgg
     2221 tgacgagcac gtcgcatggc gtactttgca acacttggat caatgaagtg ggcgtcgccg
     2281 cttcgccgac aatggtgaca ccggcatgca tttccagcgc ggcgcgaacg ccaagcagaa
     2341 cgaaaggatg atcgtccgcc agaatgactc gaaggttcac tatgatttct ctctgatgtt
     2401 gtaggattgt tcggcgaatg cgcaggagaa atgccacccg gattacgcct gctttgacgg
     2461 cctcctgact tggaataacg tgccaggggc ggccataaaa acaccgctac aacgatgcgg
     2521 aaatcggagg gttcctaaac ccgcacagaa tttgatagcc cgttgcagcg ctatcaaaat
     2581 gggaatgcat gattatgcga atcggatacg cgagagcgtt ttatctcgcg gttaattccc
     2641 ggctgaaaag caatgaagcg acgcgagcgc gtactgccgc tcccgtcgca tggaaaacaa
     2701 cgaacgaagc cggtagcgtc aggccaccgg ctacggttac ccggtcatgc ggcatcgcac
     2761 gcgcgtgccg ctgtcagctt gctagcgcga gccgcgcgcg gcaataccgg tagtgcgatc
     2821 aatgcgcaca cactggctac cgcccacacc atcggccacg aacccaccga caacaacggc
     2881 ggaatcgcga gcggcgtcag aaacagcgtg agaaagacgc aggtgttgcc catcgcgagt
     2941 gcggtacccg cgcgactcgt tcctgcaagc gtcgcgagtt ccgtgaaggc aacgccgtgc
     3001 cacgcggacc cgctgatgcc gcccagcacg atcatcagcg cgaacaccgc cgtcagcgcc
     3061 gcgtggtgca taccggcgaa gcccgtcacc aaggcaagca atgcgaacag caccgccgtc
     3121 aacacgctgc aggcacgcat gtacgcgcgt cgattgccgc gccggtcggt ccacgtcccg
     3181 ctccagaccc gcgcaatcgc agcacccgtt tgcaccgccg ccatcgtcac actgatcgcg
     3241 agcacgccgg cagcgccgaa gtcgtgaaga aacacagttc cgaacgtcac gaccgcaatc
     3301 tgcggcacgc acaatgcgcc ggcgccgagc gccactcgcc agatgcccat atcgcgtaac
     3361 ggcgaaacct tcgccggcac ggcagccgca gccgccgcat ggcgagccgg acccacgtcg
     3421 ctcccttcgg catgtgaagg ttcgtgcagc cagcaccagg cgaaccccgc ggtaatcgcg
     3481 cacgcaaggg ccagcacggt gtacacgctg gcaaacccga atcttgaggc gagcaccggc
     3541 aagatcaatg cccccaggcc gccgccggcc ggcaccgcgg tctgccggat actcatcgcg
     3601 aggccgcgct caccttcgcg aaaccagccc atcaccgcgc ggccactcga accattgacg
     3661 ctgccgccga gcagaccaac cagcaacagg ccgaacgcga gcgtggccac gttcggcaca
     3721 tgcgtgccgg acggcaccac gaagagtgcc agcccggcca gcgcggcggt agtcgaaaga
     3781 agtccgagca gcagcacgcg gcggtcgccc caccggtcgg tcaggagtcc ccatggcaac
     3841 tcgctgaccg caatgcccaa accgagcatg ccgagcacca atcccagccc atcgttgccg
     3901 aggtgatagc cagagcgcaa aaacacggcc gtggtcggaa tccccgagaa agctgccgag
     3961 aaactcgcgt tcgccgcgaa gccgacgccc agcactttcc accgatgatt tgccgcacgc
     4021 cggcccggcg ccgggccggc ggcaatcgat tcgtttccca tgacagtctc tgcaatgaat
     4081 gaattggagg tatcctacgg ctggaccaac catcggaaaa gccggaaatt cagatgactt
     4141 ccatcggata tgccgaatca tgagccaacg tggattcgac ctgacacagt tgcgcacctt
     4201 tgtcgccgtc gccgagtcgg gcagcgtctc ggcgggcgcg gagcgggtat ttctatcgca
     4261 gtcgtcggtg agcgagcagt tgaagaaact cgaggaacgc gccggccagc cgctcttcgt
     4321 acgcagcaag caaggcgtgt cggccacgcc ggccggcagc cgcctgctcg atcacgcgcg
     4381 gcgcatcatc gccatgagcg aagcggcgtt cgaagacctg caaggccgtt cgctcgacgg
     4441 tgaactgcgc atcgccatca cggactacta ccgtccgctc gacatcgcgc gcattctcaa
     4501 gacgttttcg gaacaacacc cgcgcctgaa gctgcacgtc accgtgctgc caagcgccgt
     4561 gatcgacagc agcgccggcg acgatgcgtc gtacgacatc ggcctctcgc tgcggctcgt
     4621 caccgggaac ccgcgcgccc ctggccgccg cgccggtgca cagagcaccg tggtgcggcg
     4681 cgagagattg ctgtgggtct gcgcagccga tgccagtccg cggccggcca cgccctatcc
     4741 gctggtttta ctgccttcgc gttgccagtt gcagcgcttc gtcgtgaagc ttctggacga
     4801 gcacaaggtg ccatatctgg tatcgcattc ggcctccggg gtggccgggc tgcaactcgc
     4861 attgaaggcc gggttgggga tttcatgtct gaacgaatcg tcgatcggcg ggggcgtggt
     4921 ggcctgtccg ccgagcgtcg gcttgcccgc actgcctgcc gtcgagtttc atctgctgcc
     4981 gggacgggtc ggcgaaagcg aacgcgtcac taacgcgcgc accgcattga cgcgtctttt
     5041 cagttagcgc gcggcgcgtc gatgtacgca cagcgagccg cgaatcgcga tagaaatgga
     5101 gagcggcgcg tcgatcgcat tcgccgatcc gctgcgcact taaaccacat tcagaataaa
     5161 tattgaaagc gctctaatcg acgcctatgc tgatcgagta aggcgccgct tggcctacca
     5221 attttgattg acgcatggga taaattcctg caacctgata ctcattagac agacgtatca
     5281 accatcatta atgcagcccg aaacgcgccg cccaccgcgc attcaggcag gtcatttttt
     5341 tatagagcaa tcgcgtcgcc gtatcgattc aacactgcgc ctccttaata agtccgtttc
     5401 gatttgctgt tcatgcagtc gttataaaag aaaaagtaac tggcgtgtcg tgcctcgctc
     5461 atcgaattca taagcgaatt catgcgcgca tgcgtgataa ataaatagcg aaaagaaccg
     5521 ttcccccgac gaggcctggc gtgcatcgta gaactatcgg attttcgatc ctgttgatgt
     5581 tttacgtcgt gtggcgcggc gcggcgttcg ccgccactga cgcatcgggc gcgatgccct
     5641 cggccatgga cgagcgagtg cgcgcctgca cctcgtgtca tggcgtgcaa ggccaaggcc
     5701 tgaacaacga ttacttcccc cgtatcgccg gcaaaccggc cgactatctg ttcaatcaat
     5761 tgtccgcgtt ccgtgacggt ggccgcacct atccgccgat gggttatctg ctcgccttcc
     5821 tccccgacga ctatctacgc aagatcagcc agtacttcgc cgacctgcaa ccgccctacc
     5881 ccaatgccga tcacagcgac gtcgcgccgg cggtgcttgc agccggacaa cggctcgtca
     5941 cccaaggcga cccggcacgc aagatcccgg cgtgcatcgc ctgtcacgga gcgcgcatga
     6001 cgggtacgca acctggcatt ccgggcctgc tcggtctgca tgcgagttac attgccgggc
     6061 agatgggcac atggcgctcg ggcacacgtc acgcgcttgc gccggactgc atgcattcga
     6121 tcgccgtcaa gctgaccgat aaggacatca ccgcggtatc gaactggctc gcgcgccagc
     6181 cgcgccccgc cgatccgcgg cccgcgccgg catccgctga acggctgcct ctcgcatgcg
     6241 ggagccaacc gcaatgaagc cgaccctcca cccgatccgt tcagtttctt atgcgatgac
     6301 gaagcgccgt cgcaccgctg catcgctcgt ctccggcttg ctggttgtgg gcttgatcgt
     6361 cgccggcagc gccgcgcttc ccggcgcagc gcgcgcgttg ctgatcacgg aagcacgcgc
     6421 cgctgaagcc gtacctgccg gcacgacaac agcgccggca aagaccgcag ccgccactgg
     6481 cgacaaaacc gacacccgcc ccgacatcgt caagcgcggc gagtatctgg cgcgcgccgg
     6541 cgactgcgtc gcctgtcata ccgcgccgcg cggcaagctg ttcgccggcg gcctcgcgat
     6601 ggatacgccg ttcggcacgc tgtattcgcc gaatatcacg ccggatgcgc agtacggcat
     6661 cggcacgtgg agcgaagacg cattcttcaa aatgatgcgc accggccgca cgccggacgg
     6721 caccctgctc taccccgcga tgccgatcgc ccagtacacc aaagtcacgc gtgaagactc
     6781 ggacgcgatc ttcgcgtatt tgaagtccgt gacaccggta cgcgagccga accacaaaca
     6841 cacgctgcgc ttcccgttca accagcgcaa gttgctctac ggctggcgca ccctgtattt
     6901 ccgtgaaggc gagttccagc ccgatccgac caaatccgtc gaatggaatc gcggcgcgta
     6961 tctcgtcgat ggactcgggc actgcacgat gtgtcacacc aagatcaaca tgctcggtgg
     7021 ctcgtcgcaa agcgagcagt tcgccggcgg tttgattccg gtgcagaact ggtacgcgcc
     7081 gtcgctgacg tccgacaagg acggcggcct ctgcgactgg agcatcaagg acattgtcga
     7141 cctgttgcag gcgggcattt ccgatcgcgg cgccgtgtac gggccgatgg ccgaagtcac
     7201 ctaccacagc cttcaataca tgacggaagc agacgtcaag gcgatggccg tctatctgaa
     7261 gacactgccg gacaatcgcg gccgcaagtc gggcccgtcc gcgccgacca acactaacgt
     7321 gttcgcgctc ggcgaaaaga tctacgccga caagtgcgcg ctatgccacg gcgccaaggg
     7381 cgaaggccag ttacagcact accccccgct cgccggcaac cagtcgatcg aaatggattc
     7441 cgcggtgaac ccgattcgca tcgtgttgaa tggcggtttc ccgcctggga ccatgcgcaa
     7501 tcccgagccg tacggcatgc cgcccttcgg tcaggaactg aacgataccg atgccgccgc
     7561 cgtcgtcacg tatatccgca ctgcgtgggg caaccacggc caaccggtga cgtcccgcga
     7621 ggtcaacgag ttgcgcaagg caccgctgca ttgaacctga accgctgcac tggagaatct
     7681 ctagatggaa gcgaacgata cccgcagcgc ggccccaccc acggatgaag aagtggaacg
     7741 cgtcgtcgcg gccggcccgc atggcgcgat cgccgtggcc ggcatcgcca cgctgatcgt
     7801 catggcgatc tggttcggct tttactttct cgtcttcctg ccgcgcggcg tcatccactg
     7861 actatgtcga ctgattccaa tcaccaaccg ggcagcggcc accaggtcgc cttacgcgcc
     7921 gagcgccgct gggcgatttt cgccacggcg ctgatcgtgc tgatgctcgc cgtcatcgtg
     7981 ttctcgggac tgcattgggc gatgatgccg ccgtcacggg tcgagacgat cgatccttcg
     8041 cgcctgcaac tgtccggcga attcgtcgaa gacaatctgg gcagcgccgt cgagccggac
     8101 ggctcggtgg tcgtgcgctt cattgcgcag cagtattcgt tcacgccgca atgtctgctg
     8161 gtgcccgccg acacgccgat cacgatccgc accaccagcg ccgacgtcgt gcacggcctg
     8221 ctcgtgaccg acaccaacat caacacgatg gtcgtacccg gctacgtcgc gacgttgaac
     8281 acgcgcttcg acaaacccgc cgatcacacc atgccgtgtc acgagttctg cggcttcggt
     8341 catcagacca tgtgggccca cgtcaaggtc atcgacaaag ccacgttctt cgagcaagcc
     8401 cgacaatcac ggagactcag ctgtgtttca cgctaagcga ctcgttctcg cgcatttctg
     8461 gctcgccttc atcgcgtttc tgatcgcact gctgctcggt gcctggcaga tgttggtgcg
     8521 tagcccattg gtgccgtggg tcggcgaccc cgagctctac tatcgatccg tgaccgcgca
     8581 tggctcggtg atggcttatg tgctgcccac gctggtttcg atgggcttcg gctacgcgat
     8641 cgtcgaactt gcgcttgcgc agcggctcgt cggtctgaaa tgggcgtggg ccgcgttcat
     8701 catgctggcg gtcggcgcgg tgatggcgat ggtcccggtc gcgctcggca aggcctcggt
     8761 gctctacacg ttctatccac cgatgatcgg cagcccgttc tattacctcg gtgtggtgct
     8821 ggtggtggtc ggctcgtgga tctgggtcgc gctgatgcat gtgaatctgc gtgtgtggaa
     8881 gcgcgccaat cccggcaagc ccatcccgct cgcgatgttc gccaacgtgg cgggcgcata
     8941 tctgtgggcg tggactgcga tcggcgcggc gctcgagatc ctgttccaga ttttgccggt
     9001 tgccttcggt ctgaccaata cgatcgatgc cggtctctcg cgcattctgt tctcgtggac
     9061 gctgcatgcg attgtctact tctggctgat tccggcttat atcgcgtact acacgttggt
     9121 gccgcgtgcg atcggcggac gactctatag cgattcgatg gcgcgcgtgt cgttcattct
     9181 gttcctcgtg ttcgcgatgc cgatcggcat tcaccatctg ttcgcggatc cgcaggtcgg
     9241 ctcgggcttc aagtttctgc atgctgtgtt caccgggatg gtgtcggtgc caacgctgct
     9301 gacggtgttc acgatttgcg cgtcggtgga aatcgcaggc aggctgcgcg gcggcaaagg
     9361 cgcgttcggg tggttgaccg cattgccgtg gcaaaacccg atgatgctgg tgatcgcgtt
     9421 ttcgttcgtg atgctgggcc ttggcggtgc gggcggcctg atcaacatga gctatcagtt
     9481 gaactcgacg attcacaata cgcagtgggt gacggggcac tttcatctga ttttcggcgg
     9541 ggcgatcgtc atcatgtact tcgcgatcgc gtacgagctg tggccgcaac tgaccggccg
     9601 cgcgattgcg tcggtgaagc tggtgcggac gcaactgtgg ctgtggttca tcggcatgat
     9661 ggtggtgacg ctgccgtggc actacgtcgg cttgctcggt gcgccgcgcc gtatggcgta
     9721 ttacgactac aacacgccgg ggatggaatc gcaggcgttc tgggtgggga tgtcggcggt
     9781 gggcggatta attctcgtgg tgtccggcgt gctgttcatt tacatcctcg cgaagtcgca
     9841 gttcgggccg gaggtccagc aacagagttt tcgctttgcg agcgcggtgc agccggtgga
     9901 gcatgtgccc gccgcgctca atagtttcgg cttgtgggtg gcgttgatga tcgggctgac
     9961 ggtcgtcaac tacagtgtgc cgatcgttca gctgctcaga ttgccgcaga cgtcggtgcc
    10021 ggcagttgtc gtcggagcgc agcgatgagt caggaacgcg tctttagctt gcgcaaccca
    10081 tggttcacgg tgagcctggg cggcacgatt gcgattgctg ttgtggcgat gttgattggg
    10141 ttcatctggt tgccatcggc gaatagttcg ttcagcgagc gcggcttgtg ggcgacgatt
    10201 tgcagcgccg cgggcgtgcc gtcgagctgg tacggcggcg gttcgaatat tgctggcccg
    10261 gcgccgagcg aggtggtggt gttaccgcct ttgcggcgtg cacgagccga cgcgaattcg
    10321 atcggccgcg gtgcgacgat cgcgacgcag aattgttcga tgtgtcatgg cgtgttgggg
    10381 acggtgcagg tgacggcgcc tgcgttggcc gggcaatatg ctgacgtggt ctataaagag
    10441 ctgcgcgatt atcagaacgg cgtgcggcag aacgcaatca tgcaaccgat cattgcggct
    10501 cgcagtgatc aggatttgca tgatctggcg gcttattatg cttcgctgcc gagggcgccg
    10561 gcggcggagg ttttgcctac tggcgcgcgg ccggatgcga atgctgtgaa gcttgcgatg
    10621 cagggtgatc cgcagcggaa tattgcgccc tgtgcggcat gtcatggaca gttggatcgt
    10681 aagggcgccg cgccctggct tggtggacag tcttctattt atctcgctgc gca
//
LOCUS       DATASH010000194         2712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2794381,
            whole genome shotgun sequence.
ACCESSION   DATASH010000194 DATASH010000000
VERSION     DATASH010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2794381"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="VHV99_05485"
     CDS             <1..629
                     /locus_tag="VHV99_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007582581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634930.1"
                     /translation="HRARDAIYLTAAATGVIGNGFHLYNILKRPGGLGWGNLFYAAPI
                     GAPIAMLLSGALGAAAERLRDEPERDPRLYGMPAGRALALLASVGLIGTVGEAALLHF
                     RGAFHNPAMYAPVAIPPIAAALLAHAALATPQPKRWFTRFWLRCTALLGFAGIGFHTF
                     GVARNMGGWRNRSQNIFSGPPLPAPPSFTALALAGLAALGLREGEADD"
     gene            622..1287
                     /locus_tag="VHV99_05490"
     CDS             622..1287
                     /locus_tag="VHV99_05490"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007582583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HEX3634931.1"
                     /translation="MTDQKPIPRAARYPGYDVLRKRHTPSWDETTRRVVDARLAVRRD
                     PTFLDEAAWRTLDALCGRIVPQPADRPPVPVAALVDARLQRDQRDGYRDARLPPTRVA
                     WQRGLAALDAEAQARYNVLFHQLDYGEQDALITKMQQGDLTDAAWRDMPPHLFFADRV
                     LHDICGAYYSHPASWSEIGFGGPASPRGYVRLVAGRRDPWEAVEGTPGGEDEARKENE
                     RVR"
     gene            1277..>2712
                     /locus_tag="VHV99_05495"
     CDS             1277..>2712
                     /locus_tag="VHV99_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX3634932.1"
                     /translation="MFDDALHAPRGKDGRAPDVHRVGGWLPMREYPQNEAVDFVVVGT
                     GAGGGTLACRLAQKGFSVVAFDAGAWWRPLEEFASDETHQEKVYWTDERIVDGETPLR
                     LGNNNSGKAVGGSTVHFAMVSLRFRPEWFKARTKLGYGADWPLDWREMWRYYAQVEEA
                     LKISGPVNYPWGPKRPRYPYRAHELNAAALVLACGAEALGIDWTPTPLATVSAPRGES
                     HQCVYRGFCVTGCSTNAKQSALVTWIPWAVQAGAEIRDLAMVGRIETDDAGHATGVHY
                     VRDGVWQFQQARNVVVAGYAIETPRLLLMSANAAFPDGLANSSGLVGKNLMVQTNQAA
                     WGVMDEEIRWYKGPPSLAMTEHWNYTDTGKDFFGGYCYMSQGALPAAWASTQNGRGLW
                     GDALLHEMEKYNHQVGLKIVGETLPQERNRVTLADEKDQYGLPIARVTYSLCDNDTRL
                     VRHALDFMGRHLQAAGAREIWNQENDTC"
BASE COUNT          455 a          857 c          929 g          471 t
ORIGIN      
        1 cccaccgtgc acgagacgcc atctacctga ctgccgccgc gactggcgtg atcggcaatg
       61 gttttcatct gtacaacata ttgaagcgac ccggcggact tgggtggggc aacctgtttt
      121 atgcggcgcc cattggcgcg ccgatcgcga tgctgctctc cggcgctctc ggcgcggcag
      181 ctgagcggct gcgcgacgag cccgagcgcg atccgcgcct gtatggcatg cctgccggcc
      241 gcgcgcttgc cctgctcgcc agtgtcgggc tgattggcac ggtcggcgaa gctgcgctgc
      301 tgcatttcag aggggcgttt cacaatcccg ccatgtacgc gccggttgcg attccgccaa
      361 tcgccgccgc gctgctcgcc catgccgcac tcgctacacc gcagccgaag cgctggttca
      421 cccggttctg gcttcgctgt accgcgttgc tcggttttgc gggcatcggg tttcacactt
      481 tcggggtcgc gcgcaacatg ggcggctggc gcaaccggtc acaaaacatt ttcagcgggc
      541 caccgctgcc ggcgccgcca agcttcacgg cgctcgcgct ggcgggtctc gcggcgcttg
      601 gtttgaggga aggggaagcg gatgactgat caaaagccta tcccgcgcgc tgcgcgctat
      661 cccggctacg acgtgctgcg caaacgccac acgccgtcgt gggacgaaac gacccgtcgg
      721 gtggtcgacg ctcggctggc ggtcaggcgc gacccgacgt ttctcgatga agccgcgtgg
      781 cgtacgctgg acgcgctgtg cgggcgcatc gtcccccaac ctgctgaccg gccgcccgtg
      841 ccggtcgcgg cgctcgttga tgcgcgcctg cagcgcgacc aacgcgacgg ctatcgcgat
      901 gcgcggctgc cgccaactcg cgtcgcgtgg cagcgcggcc ttgcggcact cgatgccgaa
      961 gcgcaggccc gttacaacgt tttgtttcat cagctcgact acggcgagca ggacgccctc
     1021 attacgaaaa tgcagcaggg cgacctgacg gatgccgcct ggcgcgatat gccgccgcac
     1081 ctgttcttcg cggatcgggt cctgcacgac atctgcggcg cgtactactc gcaccctgcc
     1141 tcgtggagcg agatcggttt cggcggtcct gcaagtccgc gcgggtatgt gcgtctggtc
     1201 gcaggcagac gcgacccctg ggaagcggtc gaaggcacgc cgggcggtga agacgaagcc
     1261 cgcaaggaga acgaacgtgt tcgatgacgc gttgcatgct cctcgcggca aggatggccg
     1321 ggcgcctgat gtgcaccgcg tcggtgggtg gctgccgatg cgcgaatatc cgcagaacga
     1381 ggccgtcgac ttcgtcgtcg tcggcacggg tgcgggcggc ggcacgctcg cgtgccggct
     1441 ggctcaaaag ggcttttcgg ttgtggcctt cgacgccggc gcctggtggc ggccgctcga
     1501 agagtttgcc tcggacgaaa cgcatcagga gaaagtctat tggaccgacg aacgcatcgt
     1561 cgacggtgaa acgccgctgc gccttggcaa caataacagc ggcaaggcgg tcggcggcag
     1621 caccgtccac tttgcgatgg tgtcgctgcg ctttcggccc gagtggttca aggcgcgcac
     1681 gaagcttggg tacggggcgg attggccgct ggactggcgc gaaatgtggc gctactacgc
     1741 gcaggtcgaa gaggcattga aaatctcggg ccctgtcaat tatccatggg gtccgaagcg
     1801 tccacggtac ccctatcgcg cccacgaact gaacgctgcg gcgctggtgc tggcgtgcgg
     1861 cgccgaagcg ctgggcatcg actggacacc gacgccgctc gccacggtgt cggcgccgcg
     1921 tggcgaatcg catcagtgcg tttatcgcgg tttttgcgtg acgggctgtt caacgaatgc
     1981 gaagcagagc gcgctcgtga cctggattcc ctgggcggtg caggcgggcg ccgagatccg
     2041 cgatctggcc atggtcggcc gtatcgaaac ggacgacgcg gggcacgcga ccggtgtgca
     2101 ctacgtgcgc gatggcgtgt ggcagttcca gcaggcgcgt aacgtggtgg tcgcaggcta
     2161 tgcgatcgag acgccgcgcc tgctcctgat gtccgccaac gcggcgtttc ccgacgggct
     2221 tgcgaacagc tcgggactgg tcggcaagaa cctgatggtg cagaccaacc aggcggcatg
     2281 gggcgtgatg gacgaagaaa tccgctggta caaggggccg ccctcgctcg ccatgacaga
     2341 gcactggaac tacaccgaca cgggcaagga cttcttcggc ggatactgct atatgagcca
     2401 gggggcactt ccggctgcat gggcgtcgac gcagaacggt cgcggcctgt ggggcgatgc
     2461 gctgcttcat gagatggaga aatacaacca tcaggtgggg ttgaaaattg taggcgagac
     2521 cctgccgcag gagcgaaacc gcgtcaccct tgctgacgag aaagaccagt acggcctgcc
     2581 gatcgcccgc gtcacctatt cgctatgcga caacgacacg cgcctcgtgc gacacgcgct
     2641 ggacttcatg gggcggcatc tgcaggccgc cggcgcgcgc gaaatctgga atcaggagaa
     2701 cgatacctgc ca
//
LOCUS       DATASH010000195         5775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2399992,
            whole genome shotgun sequence.
ACCESSION   DATASH010000195 DATASH010000000
VERSION     DATASH010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5775
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2399992"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="VHV99_05500"
     CDS             complement(<1..714)
                     /locus_tag="VHV99_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX3634933.1"
                     /translation="MSRSFKPYPFTPRHYAGVVPPLIDGRDPVRRAVAIVGGGPVGMT
                     LALALARQGVRSVLIEADDGVCTGSRAICISRRSLEIFKRLGAVHGFLQKGLPWTGGR
                     SFYRDAEVFRFTMHQDDEQSLPPMINIAQYQIEQLLLDEVEQHTDLIDLCWQTRVTGI
                     EQHADDGAAGATLDLRTGDTTWKMHADWVVACDGGRSTIREALGLKLTGTTYEGRYVI
                     VDIELKSERATERLAYFDPP"
     gene            complement(1009..2961)
                     /locus_tag="VHV99_05505"
     CDS             complement(1009..2961)
                     /locus_tag="VHV99_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX3634934.1"
                     /translation="MKISLPPHGRPNRVYPPGTPSLHGLASVITGVVVVCALYFGRAV
                     LIPITLSVLLSFLLAPLVQALRRLHMGQLPSIFIAVLFALATLLAVGGLIVAQLVQLA
                     GDLPQYQVAIEQKIETVQEKTVGRADSLMSRAAATLQRVAPSKPNPPRPTGRAARNAP
                     AVPQPVEVHEPSPTPMQLAQRALSPAIAPIETTFIVLVVTIFILLQREDLRDRLISLF
                     GSRDLHRTTTAINDAAVRLSRYFVAQLGVNLSAGGIIAIGLAIIGVPGALLFGVIAAL
                     LRFVPYIGIWIAAILAVFLAAAIQPQWTMAVYTLILFIVVDLVAGQIAEPLLYGHRSG
                     LSPLAVVVAAIFWSWLWGPVGLVLSTPLTLCVVTLGRYADRLNFLTVLLGDQPALTPA
                     QNFYQRLLADDPHEAIVQAERLLREMSLIDYYDKVALEGLKLARNDAMRGVLMPDQLA
                     RVNDALVDIVENLEIADGLPDRLSRTDSADAAEALLASASADLSERHDSDVSARQHAR
                     HEADRTSVLCVPGRGAFDEVTTAIAVQLLGRQGLAPMMATHSGYRSARADEPSFKDAP
                     IICIISLDAPDSPPYLRNMLRRTREWRPRATLVVGVGGAPEGGPEMGATGASHTAPTF
                     RAMIEECQAGASLQHARQPSHAAGAD"
     gene            complement(3093..4373)
                     /locus_tag="VHV99_05510"
     CDS             complement(3093..4373)
                     /locus_tag="VHV99_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018438130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HEX3634935.1"
                     /translation="MNKETELKQAKRTPLLLLAAAACVFVVTAFMPRGIWVDGIKAVA
                     EAAMVGALADWFAVVALFRRVPIPIVSAHTAIIPKNKHKIADNLAVFVQDKFLDVPSL
                     VGLIQKHDPAHSITAWLTTPANTARLGDYVVRLTGGILELTDDVRIQVFIKDALRDVL
                     AKVDLSQSMGAILDTLTKDGRHQELLDAGIEQVMALLREPQAREFIAERIVEWVKSEY
                     PKMEKILPSAWLGEKGAEAIANAVNRMLEQISENPTHQLRQKFDDAAQKLIDKLKHDP
                     AFLQKGEELKRYLMEGDALSTYVKDMWGELRAWLKRDLQSSESALHARVMAMGQWVGR
                     ELAHDPALRQSLNDHLEEAARAMAPDFAQFLTRHISDTVKNWDAREMSRQIELNIGKD
                     LQYIRINGTLVGGFIGLLLYASSQLFELLRLHMG"
     gene            4600..5742
                     /locus_tag="VHV99_05515"
     CDS             4600..5742
                     /locus_tag="VHV99_05515"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HEX3634936.1"
                     /translation="MIRFLHTADWQIGTQFGQFEPDEAAHLAEARFETVRRIAGEAAA
                     RQVDAVLVAGDVFDLQTVSDTVIRRLFGALQAFPGPWIMLPGNHDAALVESVWTRAQR
                     LNCMASNVRVVLEPGVVTLDACKGALLCAPLTQRITYDDTTGFFDTTPTPPGYHRIGL
                     AHGSVSGILQEGIDSSNPIAPTRAASARLDYLALGDWHGHLRVDERTWYAGTHEQDRF
                     RANDPGFMLDVTLTEPGAAPVVEAVRVGKYQWHRWEETISVPSDVDSLKLRLGALGSQ
                     DVLRVTVSGTTALAEAEAIHVAVEETRARVRALRVDLSGLQVLPTADDLAELGAQSGY
                     LAKVVARLRGLQEDPQSDPQQVKRAAEALLLLARFQRELPREARSA"
BASE COUNT         1022 a         1846 c         1895 g         1012 t
ORIGIN      
        1 gggcggatcg aaataggcga gacgctcggt ggcgcgctcg ctcttcagtt caatatcgac
       61 gatcacgtag cggccttcgt acgtggtacc ggtgagtttg aggccgagcg cctcacgaat
      121 cgtgctgcgg ccgccgtcgc atgcgacgac ccagtccgcg tgcattttcc aggtggtgtc
      181 gccggtgcga aggtccagcg tagcgccggc ggcgccatcg tcggcatgtt gctcgatgcc
      241 ggtcacgcgg gtttgccagc agaggtcgat cagatcggtg tgctgttcca cttcgtcgag
      301 cagcaactgt tcgatctggt attgcgcgat gttgatcatc ggcggcagcg attgctcgtc
      361 gtcctgatgc atcgtgaagc ggaacacttc ggcgtcgcgg tagaagctgc ggccgcccgt
      421 ccatggcaag cccttttgca ggaagccgtg cacggcgccg aggcgtttga aaatttccag
      481 gctgcgccgg gaaatgcaga tcgcgcggct gccggtgcag acgccgtcgt cggcctcgat
      541 cagcaccgag cgcacgccct ggcgagccag cgcgagcgcc agcgtcatgc cgaccggccc
      601 gccgccgaca atggcgacgg cgcgccgcac tgggtcgcgg ccgtcaatca gcggcggcac
      661 cacgcccgca tagtgacgag gcgtgaacgg ataaggtttg aagctgcggc tcatgcgtgt
      721 ctcccggatc ttcgatgcct atggactgcc cgccgcggcc tgagtgcact ctggaggcgg
      781 tgctaatcga taggtgaagt atggaggagg ggcggcgcgc ggctgtcctc attttgggta
      841 catgacctgg cgcgttggag tttgcgcttc ggatcgtgga ctcggctcta agcgcgcggc
      901 acacggggca cggcccacag cccacagccc acgctacgcg gccacggcac atgccacacg
      961 gcaccacggc accacggcac acggcacgcc acgctgccgc agaccagttc agtcagcccc
     1021 agccgcatgc gaaggctgcc gcgcgtgctg caggcttgca cccgcctggc attcctcgat
     1081 cattgcgcgg aacgtcggcg ccgtatgcga agcaccggtc gcgcccatct ccggcccgcc
     1141 ttccggtgcg ccgcccacgc ccaccaccag tgtcgccctg ggtcgccatt cccgcgtgcg
     1201 ccgcagcata ttgcgcagat acggcggcga gtccggcgcg tcgagcgaga tgatgcagat
     1261 gatcggcgcg tccttgaagc tcggttcgtc ggcacgcgcg ctgcgatagc ccgagtgcgt
     1321 cgccatcatc ggtgcgagcc cctggcgccc gagcaattgc acggcgatcg cggtggtgac
     1381 ctcatcgaat gcgccacgtc cgggcacgca cagcaccgac gtcctgtcgg cttcatggcg
     1441 cgcgtgctgg cgtgccgaga cgtcgctgtc atgccgctcc gaaagatcgg ctgaagccga
     1501 agccagcagc gcctcggcgg cgtcggccga gtcggtgcgc gacaggcggt ccggcagtcc
     1561 gtcggcgatt tccaggtttt cgacgatatc gaccagcgcg tcgttgaccc gcgccaactg
     1621 gtcgggcatc aacacgccgc gcatcgcatc gttgcgcgcg agcttgaggc cttccagcgc
     1681 gaccttgtcg tagtaatcga tcagcgacat ctcgcgcagc aggcgctcgg cctgcacgat
     1741 ggcttcatgc ggatcgtccg ccagaagcct ttgatagaag ttctgcgcgg gcgtgagcgc
     1801 gggctgatcg cccagcagca ccgtgagaaa gttcagacga tcggcatagc ggcccagcgt
     1861 gacgacgcac agcgtgagcg gcgtggagag cacgagcccg actggccccc acagccagct
     1921 ccagaaaatc gccgccacca ccaccgcgag cggcgacaga cccgagcgat ggccgtatag
     1981 cagcggctcg gcgatctgcc cggccaccag atcgacgacg ataaacagga tcagcgtata
     2041 gaccgccatg gtccattgag gctggatggc ggcggcgaga aacaccgcga gaatcgcggc
     2101 gatccagatg ccgatgtatg gcacgaaacg cagcaacgcg gcgatcacgc cgaacagcag
     2161 cgcccccggc acgccgatga tcgccagacc aatcgcgatg atgccgcccg cgctgagatt
     2221 cacgccgagt tgcgcgacga aatagcggct cagccgcacg gcggcgtcgt taatcgcggt
     2281 ggtcgtgcgg tgcagatcgc gcgaaccgaa caggctgatc aaacggtcgc gcagatcctc
     2341 gcgctgcagc aagatgaaga tcgtcacgac cagcacgatg aaggtggtct cgatcggtgc
     2401 aatcgccggt gatagcgcac gctgcgcgag ttgcatcggc gtgggcgaag gctcgtggac
     2461 ttcgacgggc tgcggcacgg cgggcgcatt gcgcgccgcg cgtccggtgg ggcgcggcgg
     2521 gttcggcttg ctcggcgcga cccgttgcag cgtggcggcg gcacggctca tcagcgaatc
     2581 ggcccggccg acggttttct cctgcacagt ctcgatcttt tgctcgatcg ccacctgata
     2641 ctgcggcaga tcgcctgcca gttgcaccag ttgcgccacg atcaagccac ccacggccag
     2701 caaggtggcg agggcgaaca gcaccgcgat aaagatcgac ggcaattgac ccatgtgcaa
     2761 gcgccgcaat gcctgcacca gcggtgcgag caaaaagctg agcagcacgg agagggtgat
     2821 cgggatcaac accgcgcggc cgaagtacag cgcgcatacc accaccacgc cggtaatcac
     2881 cgacgccaaa ccgtgcaggc tgggcgtgcc tggcgggtat acgcggttcg gccgtccgtg
     2941 cggcggtaac gatattttca tgagcacact tctcgatgtt gacgctgtcg cttccatgag
     3001 gggcgcgcga atggaacggc ggcagtgaga ctagcgcccg agcaattcat atgcctgaat
     3061 tgtaggcgct ttgatgcgcg gcgaaccggc ccttagccca tgtgcaaacg cagcagctcg
     3121 aacagctgcg aactcgcata cagcagcaag ccgataaagc cgcccaccag cgtgccgttg
     3181 atgcggatgt actgcagatc cttgccgatg ttcagttcga tttgccgtga catctcgcgc
     3241 gcgtcccagt tcttcactgt atcgctgatg tggcgcgtga ggaactgggc gaaatccggc
     3301 gccatggcgc gtgccgcttc ttccaggtgg tcgttcagcg actgacgcag cgcggggtcg
     3361 tgcgccagtt cgcggcccac ccattggccc atcgccatca ctctggcgtg cagcgccgat
     3421 tcgctgcttt gcagatcgcg cttgagccat gcgcgcaatt ccccccacat gtccttcaca
     3481 tacgtgctga gcgcgtcgcc ttccatcaga taacgcttca gctcctcgcc tttctgcagg
     3541 aacgccggat cgtgcttgag tttgtcgatc agcttctgcg ccgcgtcgtc gaatttctgg
     3601 cgcagttgat gggtgggatt ctcgctgatc tgctcgagca tccggttcac cgcattggcg
     3661 atcgcctcgg cgcctttttc gccgagccac gccgacggca ggattttttc catcttcgga
     3721 tattcgctct tcacccactc gacgatgcgc tccgcgataa actcgcgcgc ctgcggttcg
     3781 cgcaacagcg ccatgacctg ttcgatgccg gcgtcgagca attcctggtg acggccgtct
     3841 ttggtgagcg tatcgagaat cgcgcccatg gattgcgaca gatcgacttt ggccagcacg
     3901 tcgcgcaatg cgtccttgat gaagacctgg atgcgcacgt cgtcggtcag ttcgagaatg
     3961 ccgcccgtca gcctgacgac gtagtcgccc agacgcgcgg tgttggccgg cgtggtcagc
     4021 caggcggtaa tgctgtgcgc cgggtcgtgc ttctggatca ggccgaccag cgacggcacg
     4081 tcgagaaact tgtcctgcac gaagaccgcg aggttgtcgg cgatcttgtg cttgttcttc
     4141 ggaatgatcg cggtgtgcgc ggagacgatg ggaatcggca cgcggcgaaa cagggcgacc
     4201 acggcgaacc agtcggctag cgcgccgacc atggcggcct cggccaccgc tttgatgccg
     4261 tccacccaga tgccccgcgg catgaaggcc gtcaccacga aaacgcaggc agcggccgcc
     4321 aacagcagca acggggtgcg cttggcttgc ttcagttcgg tttctttgtt catcggcagc
     4381 agggcggcgg agttcaagtc gattgaaatg gtgaggtgcg attggcatgc tcgtccagaa
     4441 aacgcgagtg tagcgccgct gttccggcag ttggccctgg ccttggacat tctttgatcc
     4501 gctggcgcgg taccatctgc ggttagactg cgtggcgacg cttgaacgag cactgcatgc
     4561 ccgctgaatg acgatgcaat tgaacggaag ggaggcacat tgatcaggtt cctgcacact
     4621 gcggactggc agatcggcac gcaattcggc cagttcgagc cggacgaagc cgcgcatctc
     4681 gcggaagcac gcttcgaaac cgtgcgccgg atcgccgggg aagcggcggc gcgccaggtc
     4741 gacgccgtgc tggttgccgg cgacgtattc gacctgcaga ccgtatcgga cacggtgatc
     4801 cgccgtttgt tcggcgcgct gcaagccttc ccgggtccat ggatcatgct gcccggcaac
     4861 cacgacgccg cgctggtcga gagcgtgtgg acccgtgcgc agcgactgaa ctgcatggcg
     4921 tccaacgtgc gggtggtgct cgaacccggc gtggtgacgc tcgacgcttg caagggcgca
     4981 ttgctctgcg cgccactcac gcaacgtatc acctacgacg acacaaccgg cttcttcgac
     5041 acaacaccga cgccgcccgg ctatcaccgc atcgggctcg cgcatggcag cgtgagcggg
     5101 attctgcagg aagggatcga ttcgtcgaac ccgatcgcgc cgacgcgtgc cgcgagcgcg
     5161 cgtctggact atctcgcgct tggcgactgg cacggccatc tgcgtgtcga cgagcgcacc
     5221 tggtatgccg gcacgcatga gcaggaccgg ttccgcgcca acgatcccgg cttcatgctc
     5281 gacgtcacgc tgactgagcc aggcgccgcg ccggtggtcg aagcggtgcg ggtcggcaaa
     5341 tatcaatggc atcgctggga agagacgatt tcggtgccgt ccgatgtgga ttcgctcaag
     5401 ctgcgcctcg gcgcgttggg cagtcaggac gtgctgcggg tcaccgtcag cggcacgacc
     5461 gctttggccg aggccgaagc gattcacgtc gcggtggaag aaacccgcgc gcgggtgcgt
     5521 gcgctacgcg tcgacctgag cggcctgcaa gtgctgccca ccgccgacga ccttgccgaa
     5581 ctcggcgcgc aaagcggcta cctggcgaag gtggtggcgc gcttgcgcgg cctgcaggag
     5641 gacccgcagt ccgacccgca acaggtcaaa cgcgcggcgg aggcgttatt gctgctggcg
     5701 cgttttcaac gtgaactgcc gcgcgaagcg aggtccgcat gaagctggaa agtatcgcga
     5761 ttcaggagtt caagc
//
LOCUS       DATASH010000196         4368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2400058,
            whole genome shotgun sequence.
ACCESSION   DATASH010000196 DATASH010000000
VERSION     DATASH010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4368
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2400058"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(632..1192)
                     /locus_tag="VHV99_05520"
     CDS             complement(632..1192)
                     /locus_tag="VHV99_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006053313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX3634937.1"
                     /translation="MALSLNDIRSLFEQYGNLAYSGEPVTQLEHALQSGALAEEEGAD
                     DELVSAAFLHDLGHLLNLQGETPTGRGIDDLHQYFALPFLRPVLSDAVLEPIRLHVDA
                     KRCLCAIDDTYFGQLSADSVRSLELQGGIFSKEEAEAFLQKPYAEDALRLRKWDDRAK
                     EENRATPGLDHYLGVVERVMQKRLAG"
     gene            complement(1197..2648)
                     /gene="phnY"
                     /locus_tag="VHV99_05525"
     CDS             complement(1197..2648)
                     /gene="phnY"
                     /locus_tag="VHV99_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007587367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde dehydrogenase"
                     /protein_id="HEX3634938.1"
                     /translation="MNAVLQDHPAFRAEALRLKGERATRSRTLDVFDPYTGTRVGTVP
                     LASIDDVRAAFDYAAAYQATLTRYERAQILERAAALLRERTEDASDLISLESGLSKQD
                     SRYEIGRVADVLKFASIEALRDDGQSFSCDLTPHGKKRRVFSQREPLAGVIVAITPFN
                     HPMNQVAHKIAPAIATNNRVVLKPSEKVPLSALYLADILYEAGLPTPMLQVLTGDPRE
                     IADELITHPLAELVTFTGGVAIGKYIASKAAYRRIVLELGGNDPLIVLEDADLDRAAT
                     LAVQGSYKNSGQRCTAVKRMLVQKSIAADFTDLVVEKTRAWTYGDPFDASNQMGTVID
                     VAAAQLFEARVNEAIAAGARLRIGNQRVGALYAPTVLDGVDPSMALVREETFGPVSPV
                     ITFDTLDDAIRISNGTPFGLSSGVCTNRQDAITRLINELRVGTVNVWEVPGYRIELTP
                     FGGIKDSGLGYKEGVQEAMKSFTNLKTFSLPWE"
     gene            complement(2645..3919)
                     /gene="phnA"
                     /locus_tag="VHV99_05530"
     CDS             complement(2645..3919)
                     /gene="phnA"
                     /locus_tag="VHV99_05530"
                     /EC_number="3.11.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047400 - phosphonoacetate hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019636 - phosphonoacetate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetate hydrolase"
                     /protein_id="HEX3634939.1"
                     /translation="MENANTNASASERTIEVNGRSYRLPSQPTVVVCVDGCEYDYLEA
                     AVAAGVAPFLGKMIEGGAAFKGDCVIPSFTNPNNLSIVCGVPPSVHGICGNYFWDPDA
                     NGGEGAEVMMNDPAYLRAGTLLAAAAEAGAAVAVVTAKDKLRRLLGWQLKGICFSAEK
                     ADKVTREENGIDEVLDLVGLPVPDVYSAGLSEFVFAAGVRLAETRKLDLMYLSTTDYI
                     QHKWAPGTQGANDFYAMMDGYLAKLDALGWMIGLTADHGMNAKHDPKTGEPNVIYLQD
                     VLDDWLGASKARVILPITDPYVVHHGALGSFATVYLPNDVSVGEVIERIGGLQGIEVV
                     LDNRAACERFELPNDRVGDIVVVSTKQVVLGTRRDEHDLSGLTVPLRSHGGISEQEVP
                     LLFNRRVEGLPTDKRLRNFDIFDVALNQVSAS"
     gene            complement(3977..>4368)
                     /locus_tag="VHV99_05535"
     CDS             complement(3977..>4368)
                     /locus_tag="VHV99_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEX3634940.1"
                     /translation="QKLAVLREWLDAMRASLDAQDHDAYARANVAFHDAIVAASGNVK
                     LYETYRRLVSELSLFRRAALVVHADAMERSLAEHRAILTALASRDAEHAAALMQAHVN
                     GGLQRAREACEPAGPLGAVRMPVASND"
BASE COUNT          857 a         1454 c         1211 g          846 t
ORIGIN      
        1 tgtaaggcat gccgccagcg ttcaatctga gccaggatca aactcttcag ttcaaacctg
       61 ttactgtttt tcggttcagt taagaaccgg tcgctcactc aacgtactga cgaatgatcc
      121 tcctgccggc gtgtttccac cacaacagga aaaccttcct ttcattactg tgtgagactt
      181 gatactttcg cttatgccag aacccgaagg ttccggcgcg cgtcgcgcat caagcgccca
      241 cacttatcgg ctgttaattt ttaaagatcg agtccgcatt caccaccgga accgcaccgc
      301 gcgctgctgg catcaatcaa caccacaacc acccggtacc gcttcgttct gcgtcgctgc
      361 atcagcagca gagaaacgag attatgaaga actttcgcta cgtcgtcaac aggtttttcc
      421 aactttctca acccgcccac ttcgctgaaa gccttgccac tgctggctct ccagcctccc
      481 gtgccccgtt gtccgaagca cgaaagagcg agattctagc gagccgaacc gtgccttgca
      541 agcgttattt tgatacgcat agattgcacg cgaaatggcg cgaagatgac gcaacaagcg
      601 cacccgaaga tcagcggaaa aaccatctcg ttcagccggc caagcgcttc tgcatcacgc
      661 gctcgactac gcccaaatag tgatcgagac ccggcgtcgc gcgattctct tcttttgcgc
      721 gatcgtccca tttgcgcaga cgcaatgcgt cttccgcgta gggcttctgc agaaacgcct
      781 cggcctcttc cttgctgaag atgccgcctt gcagttccag gcttcgcacc gaatcggcgg
      841 aaagctggcc gaagtacgta tcgtcgattg cacacaggca gcgcttggcg tcgacgtgca
      901 gccggatcgg ctccagcacc gcatccgaca acaccggacg caagaacggc agcgcgaaat
      961 actgatgcag atcgtcgatg ccccgccccg tcggcgtctc gccctgcaga ttcagcagat
     1021 gcccgaggtc gtgcaaaaac gcagcggaga cgagctcgtc gtcggcgccc tcttcttccg
     1081 ccaacgcgcc gctttgcagc gcatgttcaa gctgcgtcac cggctcgccg ctataggcga
     1141 ggttgccgta ttgttcaaat aacgaacgga tgtcgttcaa actcagtgcc acactcttac
     1201 tcccacggta atgaaaaggt cttcaggttg gtgaagctct tcatcgcctc ctgaacgcct
     1261 tccttatagc caagcccgga atccttgatg ccgccgaacg gcgtcagctc gatccggtat
     1321 cccggcactt cccacacgtt gaccgtcccc acgcgcaatt cgttgatcaa gcgcgtgatt
     1381 gcgtcctgcc ggttcgtgca caccccggac gacagcccga acggcgtccc gttactgata
     1441 cggatggcgt cgtcgagcgt gtcgaaggtg atgaccggcg acacggggcc gaaggtttct
     1501 tcacgcacca gtgccatcga cggatcgacg ccatccagca ccgtcggcgc atacagcgcg
     1561 ccgacgcgtt gattgccgat ccgcaagcgc gcgccggccg cgatcgcctc gttcacacgc
     1621 gcctcgaata gctgtgcggc ggcgacgtcg atcacggtac ccatctgatt tgatgcatcg
     1681 aacggatcgc cataggtcca tgcacgcgtc ttctccacga ccagatcggt gaaatccgcc
     1741 gcgatactct tttgcaccag catccgcttg accgcggtac agcgttggcc ggagttctta
     1801 tacgaacctt gcaccgcaag cgtcgccgcg cgatcgagat cggcgtcttc gagcacgatc
     1861 agcggatcgt tgccgcccaa ttccagcacg atgcgccggt aggccgcctt agacgcgata
     1921 tatttgccga tcgccacgcc gcccgtgaaa gtgacgagct cggccagcgg atgcgtgatc
     1981 agctcatcgg cgatttcgcg cggatcgccg gtcagcacct gcagcatcgg tgtcggcaaa
     2041 cccgcttcat aaagaatgtc ggccagatac agcgccgaca gcggcacctt ttccgacggc
     2101 ttgagcacca cacgattatt cgtcgcgatc gccggcgcaa ttttgtgcgc gacctggttc
     2161 atcggatggt tgaacggcgt aatcgcgacg atcaccccag ccagcggctc acgctgcgaa
     2221 aacacacgac gcttcttccc atgcggcgtg agatcgcagg aaaaactctg cccgtcgtca
     2281 cgcaaggctt cgatcgaggc gaacttgagc acatcggcaa cgcggccgat ctcgtagcgc
     2341 gaatcctgct tcgacaaacc cgactcgagc gaaatcaggt ccgacgcgtc ctcggtccgc
     2401 tcccgcagca gtgcagcggc acgctcaaga atctgcgcac gctcgtaacg cgtgagcgtc
     2461 gcctgatacg ccgcggcata gtcgaacgcg gcacgcacgt cgtcgatgct cgccaacggg
     2521 accgtgccca cccgcgtccc cgtataagga tcgaacacgt cgagcgtgcg cgagcgcgtc
     2581 gcgcgctcgc ccttgagccg cagcgcttcc gcccgaaacg ccggatggtc ctgcagcacg
     2641 gcgttcatga tgccgatacc tgattcaatg cgacgtcgaa gatatcgaag ttgcgcaacc
     2701 gcttatcggt gggcaatcct tcgacgcggc gattgaacag cagcggcacc tcctgctccg
     2761 aaatcccgcc gtgcgagcgc aacggcaccg tcagtccgga cagatcgtgt tcatcgcgac
     2821 gcgtgccgag caccacttgc ttggtgctga ccaccacgat gtcgccgacg cggtcgttcg
     2881 gcagctcgaa gcgctcgcac gcggcgcgat tgtcgagcac gacttcaatg ccctgcaaac
     2941 caccaatccg ctcgatcacc tcgccgacgc tcacatcgtt cggcaggtaa accgtcgcga
     3001 acgaaccgag cgcgccgtga tgcacgacat acggatcggt gatcggaaga atcacgcgcg
     3061 ctttgctggc cccaagccaa tcgtccagca cgtcctgcag ataaatcaca ttcggctcgc
     3121 cggttttcgg gtcgtgcttg gcgttcatgc cgtggtcggc ggtcaagccg atcatccagc
     3181 cgagcgcgtc gagcttcgcg aggtagccgt ccatcattgc gtagaagtcg ttcgcgccct
     3241 gggtgcccgg tgcccatttg tgctggatgt agtcggtggt cgacagatac atcaggtcga
     3301 gcttgcgggt ttcggcaagc cgcacgccgg cggcgaacac gaattcggac aaacccgcgc
     3361 tatacacgtc aggcaccggc aagccgacca ggtcgagcac ttcgtcgatg ccgttttctt
     3421 cgcgcgtcac cttgtcagct ttttccgccg aaaagcagat gcccttcagt tgccagccga
     3481 gcagccggcg cagcttgtct ttcgcggtaa ccacggcgac cgcggcgcct gcttcggccg
     3541 cagcggccag caaagtcccc gcgcgcagat aggccggatc gttcatcatc acttccgcgc
     3601 cttcgccgcc atttgcgtcc gggtcccaga agtaattgcc gcaaatgccg tgcaccgagg
     3661 gcggtacgcc gcacacgatc gagagattgt tcgggttggt gaacgacgga atcacgcagt
     3721 cgcccttgaa ggccgcgccg ccctcgatca ttttgccgag gaaaggcgcg acgcccgctg
     3781 cgaccgcggc ttcgaggtaa tcgtattcac agccgtcgac gcacaccaca acggtcggtt
     3841 gcgagggcag ccgatagctg cggccgttga cttcaatcgt acgttcgctt gcgcttgcgt
     3901 ttgtatttgc gttttccatc tctatcttcc atgcgtggag agcacacgtg ctccagtcaa
     3961 tccatcacaa ccagtctcaa tcgttcgatg ccaccggcat ccgcaccgcg cccaacggcc
     4021 ctgccggttc gcacgcctca cgcgcgcgct gcaggccgcc gttcacatgc gcttgcatta
     4081 gcgcggcggc atgttcggcg tcgcgcgagg ccagcgccgt caggatcgcg cgatgctcgg
     4141 cgagcgagcg ctccatcgcg tcggcatgca ccaccagcgc ggcgcggcga aacaggctca
     4201 gttcgctcac cagtcttcga tacgtctcat acagcttgac gttgcccgac gccgccacga
     4261 tcgcgtcgtg aaacgccacg ttggcgcgcg cgtaggcatc gtgatcctgt gcatcgagcg
     4321 aggcgcgcat cgcgtcgagc cactcgcgca acaccgcaag cttctgcg
//
LOCUS       DATASH010000197         2437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_25162,
            whole genome shotgun sequence.
ACCESSION   DATASH010000197 DATASH010000000
VERSION     DATASH010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25162"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /locus_tag="VHV99_05540"
     CDS             <1..1020
                     /locus_tag="VHV99_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX3634941.1"
                     /translation="QLNVYNWSDYIAKDTIPNFTKQTGVQVKYDNYDSDDTLQAKLLT
                     GNSGYDIVVPTSNYAGKQIAAGIFAPLDKSKLPNLKYLDPSLMALVAGADPGNKYTVP
                     WAYGTTGLGYNVTKVQQILGKNVPLDNWDILFKPENISKLKACGVSVLDAPDQMFAAA
                     LHYIGKDPMSTNPADYRAALEMMKKIRPYITQFNSSGYINDLVGGDVCFAYGWSGDVV
                     IAKHRAVEAKKPFKVEYYIPKGGAPVWFDVMAIPKDAKNKEAALEWINYIETPQVHAA
                     ITNAVYYPSTNLEARKYVDKDVANDPAVYPSPEVIKTLFLLKPLPPEIQRLQTRLWTE
                     FKSGR"
     gene            1113..2279
                     /gene="potA"
                     /locus_tag="VHV99_05545"
     CDS             1113..2279
                     /gene="potA"
                     /locus_tag="VHV99_05545"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010115150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HEX3634942.1"
                     /translation="MSSDQSGALAGAAAPFPGLNAKAGDAAENFIQIVDVVKKFGDTV
                     AVKGVNLSVKKGELFALLGSSGCGKSTLLRMLAGLESITSGKILIDGEDLAQLPPYRR
                     PVNMMFQSYALFPHMTVEANVAFGLKQEGVPKSELKGRVQNALDLVQMGRFAKRKPHQ
                     LSGGQQQRVALARSLVKRPKLLLLDEPMSALDKQIRQRTQIELVNILDKVGVTCMMVT
                     HDQEEAMTMADRLAVMSEGEIIQLGTPHEVYEYPNSRFSAEFIGSTNLFEGHTVEDEP
                     DHVFIETPDLPVRLYVSHGITGPLGMPVTISVRPERIALTRKPPEGAYNWGKGVVTNI
                     AYMGGYSLYHVKLDGGKTVIANVTSLALTEIESPSWGDEVYVRWSASAGVVLTS"
     gene            2276..>2437
                     /locus_tag="VHV99_05550"
     CDS             2276..>2437
                     /locus_tag="VHV99_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HEX3634943.1"
                     /translation="MKRSLSSMASWPVRRFNLTGRTAVVAGPFIWLLLFFLVPFLLVV
                     KISFADSQLG"
BASE COUNT          511 a          744 c          742 g          440 t
ORIGIN      
        1 cagctgaacg tgtataactg gtccgattac atcgctaagg acaccattcc gaacttcacc
       61 aaacagaccg gcgttcaggt caaatacgac aactacgaca gcgacgacac gctgcaagcc
      121 aaactgctca ccggcaattc cggctacgac atcgtcgtgc cgaccagcaa ttacgccggc
      181 aagcagatcg ccgccggcat cttcgcgccg ctcgacaaat cgaagctgcc gaacctgaag
      241 tacctcgatc cctcgctgat ggccctcgtg gccggcgccg atccgggcaa caagtacacg
      301 gtgccctggg catacggcac gaccggtctc ggctacaacg tcaccaaggt ccagcagatc
      361 ctcggcaaga acgtgccgct cgacaactgg gacatcctct tcaagccgga aaacatctcg
      421 aagctgaagg cctgcggcgt gtccgtgctg gacgcgccgg accagatgtt cgccgccgcg
      481 ctgcactaca tcggcaagga tccgatgagc acgaatccgg ccgactatcg cgccgcgctc
      541 gagatgatga agaagatccg cccgtacatc acgcagttca actcgtcggg ctacatcaat
      601 gacctggtcg gcggcgacgt atgcttcgcc tacggctggt cgggcgacgt cgtgatcgcc
      661 aagcatcgcg cggtcgaagc gaagaagccg ttcaaggtgg agtactacat tccgaagggc
      721 ggtgcgccgg tgtggttcga cgtgatggcg attccgaaag acgcgaaaaa caaggaagcc
      781 gcgctcgagt ggatcaacta catcgaaacg ccgcaggtgc acgcggcgat cactaacgcc
      841 gtctactatc cgagcaccaa cctcgaagcg cgcaagtacg tggacaagga cgtggcgaac
      901 gacccggccg tgtacccgtc gcccgaagtc atcaagacgc tgttcctgtt gaagccgctg
      961 ccgccggaaa tccagcggct gcaaacgcgc ctgtggactg aattcaagtc tggccgctga
     1021 agcgcggcca gagtgaacgg gtaagcatca tcatgaagcc ctcggtgtcc accgggggct
     1081 ttgttcgtca aacgccggag taatgcattg tgatgagtag tgaccagtcg ggcgcgctgg
     1141 caggggccgc cgcgccgttc ccgggcctga acgccaaagc gggcgatgcg gcagagaatt
     1201 tcatccagat cgtcgacgtg gtgaagaagt tcggcgacac cgtggcggtc aaaggggtca
     1261 acctgtcggt gaaaaagggc gagctgtttg cgctgctcgg cagttccggc tgcggcaaat
     1321 caaccttgct gcgcatgctg gccggactcg aatcgatcac gtcgggcaag attctgattg
     1381 acggcgagga cctcgcgcaa ttgccgcctt atcgtcggcc ggtcaacatg atgttccagt
     1441 cgtacgccct gtttccgcac atgacggtcg aggccaacgt cgccttcggc ctgaagcagg
     1501 aaggcgtgcc gaaatctgaa ctgaaaggcc gcgtgcagaa cgcgctcgat ctcgtgcaga
     1561 tgggccgctt tgcgaagcgc aagccgcatc aactttccgg tggccagcag cagcgtgtgg
     1621 cgctcgcgcg ttcgctcgtc aagcggccga agctgttgtt gctcgacgag ccgatgtccg
     1681 cgctcgacaa acagatccgt cagcgcacac agatcgaact ggtcaatatt ctcgacaagg
     1741 tcggcgtgac ctgcatgatg gtgacgcacg accaggaaga ggccatgacc atggcggacc
     1801 gccttgccgt gatgagcgaa ggcgagatca tccagctcgg cacgccgcac gaagtgtacg
     1861 aatatccgaa cagccgtttt tcggccgagt ttatcggctc gaccaatctg ttcgaaggcc
     1921 ataccgtcga agacgaaccc gatcacgtat tcattgaaac gcccgacctg ccggttcgcc
     1981 tttacgtgag tcacggcatt accgggccgc tcggcatgcc ggtgacgatc tcggtgcggc
     2041 ccgagcgcat cgcgctcacc cgcaagccgc ctgaaggcgc gtacaactgg ggcaagggcg
     2101 tcgtcacgaa tatcgcgtac atgggcggct attcgctgta tcacgtgaag ctcgacggcg
     2161 gcaaaacggt gatcgccaac gtgacgagcc ttgcattgac ggagatcgag tcgcccagtt
     2221 ggggcgacga agtctatgtg cgctggagcg catcggccgg cgtggtgctg acgtcatgaa
     2281 gcgctcgctc agttccatgg catcgtggcc ggtgcgccgt ttcaatctga ccggccgcac
     2341 cgcggtggtg gccggcccgt tcatctggct gctgttgttt ttcctcgtgc cgttcctgct
     2401 ggtcgtgaag atcagcttcg ccgattcgca gctcggc
//
LOCUS       DATASH010000198         2322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6949107,
            whole genome shotgun sequence.
ACCESSION   DATASH010000198 DATASH010000000
VERSION     DATASH010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6949107"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="VHV99_05555"
     CDS             <1..717
                     /locus_tag="VHV99_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX3634944.1"
                     /translation="SSVNDIMGGMFGAIGVLAALHERHTSGRGKEVQSALFENCVLLS
                     AQHMQQFAATGVAAAPMPERISAWAVYDVFRLANDEQMFIAATGDGQWRALCAILGRA
                     DLLADPRLATNNDRVLAREWLLKTLGETLSGFEGAALVPLFERDHIPFASITKPEDLF
                     DDPHLNESGGLARLTLDDGSETAMPLLPISMDGARLQPRQPIAKIGEHTADVLRGLGY
                     DEAQIAALGGGVTQPCREVE"
     gene            1079..>2322
                     /locus_tag="VHV99_05560"
     CDS             1079..>2322
                     /locus_tag="VHV99_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3634945.1"
                     /translation="MKSSNQAVYATAVGDAGALGDLPSGAGASAGGRAEEIALDGARI
                     SARLDRLPATRSIWQLVMLLSLGLFFELYDLMYSGYIAPGLVRGGILTATTHGLFGTS
                     GVASFIAALFAGLFIGTAACGFLADRFGRRAIFTWSLLWYTAANVIMAFQDTALGLNL
                     WRFIAGIGIGVEIVTIGTYISELVPKHVRGRASAFSQAVGFCAVPIVAFISYLLVPHR
                     YFGLDGWRLVVLTGVMGAIVVWWIRLRLPESPRWLAQKGRIEEADAMMKQLEARVERE
                     YGRPLPEPALPEPVRARAAFRDLLVPPYRKRTLMLIIFHVFQTMGYYGFANWIPTLLI
                     KQGITVTTSLMYASVIAIAAPLGPLLGMLIADRFERKTVIICMAAVNVVCGLLFSQAR
                     ETVLLVSLGVCLVLAGNIISYS"
BASE COUNT          395 a          657 c          792 g          478 t
ORIGIN      
        1 agttcggtca acgacatcat gggtggcatg ttcggcgcga tcggcgtgct tgccgcattg
       61 cacgaacggc atacgagcgg acgcggcaag gaagtgcaaa gcgcgctgtt cgaaaactgc
      121 gtgctgcttt ccgcgcaaca catgcagcag ttcgccgcga ccggcgtggc cgctgcgccg
      181 atgcctgagc gcatcagcgc atgggccgtc tacgacgtgt tccggcttgc caacgacgag
      241 cagatgttca tcgccgcgac cggcgacggg cagtggcgcg cgctatgtgc gattctcggc
      301 cgcgctgatc tgttggccga tccgcgcctc gcgactaaca acgaccgcgt gctggcgcgc
      361 gagtggttgc tgaagacgct tggcgaaacc ttgagtggct tcgaaggcgc agcgctcgtg
      421 ccgctgttcg aacgcgacca cattccgttc gcgtcgatca cgaaacccga agatctgttc
      481 gacgatccgc atctgaacga gagcggcggc cttgcgcgtt tgaccctcga tgacggcagc
      541 gaaaccgcaa tgcccttgct gccgatttcg atggacggcg cgcgtctgca accgcgccag
      601 ccgatcgcga agatcggtga gcacaccgca gacgtgttgc gtgggttggg ttacgacgaa
      661 gcgcagatcg cggcgttggg cggcggcgtg acgcagccgt gccgggaagt tgagtaagcg
      721 tggaggttgc tgggacggcg cccaaagccg catgagtctg gcggccggcg aggcgtcgag
      781 tcccgcaaat gaaaggatgt ggcgctcttg tccgcaggcg ctcgatgcgt cgaatgtagt
      841 caagcaacgc tcatccggcg tcagatcgga attcagcaac catttgcatg ggatcggagt
      901 aggcgctatg ggaccagagt aggcgctaaa gcgctgacct tggtcgacac gccaactccg
      961 atcgacggct ttgcatggga ccggagtaag ggctaaagcg ctaactccga tcgacagctt
     1021 tgcatgggac cagagtaagc gctaaggcgc caactctggt cgacaaagga gacagacgat
     1081 gaaatcgtcg aatcaggcgg tgtatgcgac agcggtcggc gatgccggcg cgctgggtga
     1141 tttgccctca ggcgccggcg catcggcggg aggccgcgcg gaagagatcg cgctggacgg
     1201 cgcgcgtatt tccgcgcggc tcgaccgctt gcccgcgacg cgctcgatct ggcaactggt
     1261 gatgctgttg agccttggcc tcttcttcga actatacgac ctgatgtact caggctacat
     1321 cgcgccgggc ctcgtgcgcg gcggcattct caccgcgacg acgcatggcc tgttcggcac
     1381 cagcggtgtg gcgagtttca ttgcggcgtt gttcgcgggc ctgttcattg gcacggcggc
     1441 atgcggtttt ctcgccgacc gtttcggccg acgcgcgatc ttcacatggt cgctgctgtg
     1501 gtacaccgcg gccaacgtca tcatggcgtt ccaggacacg gcgctcgggc tcaatctatg
     1561 gcgcttcatc gcggggatcg gtatcggcgt ggagatcgtg acgatcggca cgtacatctc
     1621 cgagctcgtg ccgaagcacg tgcgcggccg cgcgtcggcg ttttcgcagg cggtgggctt
     1681 ctgcgcggtg ccgatcgtcg cgtttatctc gtacttgctg gtgccgcacc gttatttcgg
     1741 gctcgacggc tggcgtctgg tcgtgctgac cggtgttatg ggcgcgatcg tcgtgtggtg
     1801 gattcgtctg cgtttgccgg agagcccacg ctggctcgca cagaaaggcc gcatcgagga
     1861 agccgatgcg atgatgaagc agctcgaagc acgtgtcgaa cgcgaatatg gacggccttt
     1921 gccggagccg gcgttgcccg aaccggtgcg cgcgcgtgcg gcgtttcgcg atctgctggt
     1981 gccgccgtat cgcaaacgca ccctgatgct gatcattttc catgtgtttc agacgatggg
     2041 ttattacggc ttcgcgaact ggattccaac gttgttgatc aagcagggca ttacggttac
     2101 gacgagtttg atgtacgcga gcgtgattgc gattgcagcg ccgcttgggc cgttgttggg
     2161 catgctgatt gccgaccgct tcgagcgaaa gacggtgatt atctgcatgg cggccgtgaa
     2221 cgtggtgtgc gggctgttgt tcagtcaggc gcgggagacg gtgctgctgg tgagtctggg
     2281 tgtttgcctc gtgctggcgg gcaatattat ttcttatagc ta
//
LOCUS       DATASH010000199         2850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4377980,
            whole genome shotgun sequence.
ACCESSION   DATASH010000199 DATASH010000000
VERSION     DATASH010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4377980"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..122
                     /locus_tag="VHV99_05565"
     CDS             <1..122
                     /locus_tag="VHV99_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006052787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HEX3634946.1"
                     /translation="SAIKGGVRADYFWGLGDDAGDLAGKMKQGGRMWLLLPNS"
     gene            complement(264..1568)
                     /gene="paaK"
                     /locus_tag="VHV99_05570"
     CDS             complement(264..1568)
                     /gene="paaK"
                     /locus_tag="VHV99_05570"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="HEX3634947.1"
                     /translation="MTTALPLDPIETASRDELAALQLERLKWSLNHAYENSPVYRRKF
                     DEAGVRPSEVKTLADLARFPFTTKKDLRDSYPFGMFAVPQEQVSRIHASSGTTGKPTV
                     VGYTARDIDTWANLVARSIRAAGARRGDKVHVSYGYGLFTGGLGAHYGAERAGLTVIP
                     FGGGQTEKQVQLIQDFRPDIIMVTPSYMLSIADELERQGIEPANCSLRIGIFGAEPWT
                     NDMRQAIEKRMGIDAVDIYGLSEVMGPGVASECVETKDGPTIWEDHFYPEIIDPETGE
                     VLPDGELGELVFTSLTKEALPIVRYRTRDLTRLLPGSARTMRRMEKITGRSDDMMIVR
                     GVNVFPTQIEELLLKQHALAPHYQIVLTKEGPLDVLTLNVEPCPESAPDTAALTTAKQ
                     ALTYDIKALIGVSAIVNVLGVNGIERSVGKARRVVDKRKSGL"
     gene            complement(1730..2191)
                     /gene="paaI"
                     /locus_tag="VHV99_05575"
     CDS             complement(1730..2191)
                     /gene="paaI"
                     /locus_tag="VHV99_05575"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HEX3634948.1"
                     /translation="MSTQSNHPAQMTPDELARATAVAMYENDACSRALGLEIVEVRPG
                     YARLRMAVRDDFLNGHQICHGGLIFTLADSTFAFACNTYNINTVAAGCSIEFLRPVHG
                     GDMLTAEAVEQTLSGRTGIYDIRVMNRAEETVAMFRGKSAQIKGNLIPSGD"
     gene            complement(2193..>2850)
                     /gene="paaG"
                     /locus_tag="VHV99_05580"
     CDS             complement(2193..>2850)
                     /gene="paaG"
                     /locus_tag="VHV99_05580"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006029796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HEX3634949.1"
                     /translation="EASGARALVLTGAGRGFCAGQDLADLDFTPGAMTDLGELIDTHF
                     NPLIRRLQALPLPVIAAVNGTAAGAGANLALACDLVLAARSASFIQAFVKIGLVPDSG
                     GTWFLPQRVGMARALGLAITGDKLSAEKAENWGLIWQTVDDTELAATASKLAAQLAQQ
                     PTRAIAMIKQAMRVGATQTLDHQLDLERDLQRELGASLDYAEGVQAFVEKRAPRFEGR
                     "
BASE COUNT          494 a          860 c          896 g          600 t
ORIGIN      
        1 ggtcggctat caagggcggg gtgcgcgccg actacttctg gggtctcggc gacgacgctg
       61 gcgatctggc cggcaagatg aagcagggcg gacggatgtg gttgttgctg ccgaattcgt
      121 gagggtttcg gcggcggcgc ggtgacgggt tttttagtcc gcgccgtatt cgccgttcag
      181 ggcccgcgcc aattgataat ggcgcgggcg ccggtcagtt cccttatcaa gcacaagcat
      241 caacatcaac ttcgacgctt cgcttaaagt ccggatttcc gtttgtccac cactcggcgt
      301 gcttttccta ccgaacgctc gataccgttc acgcccagca cattcacgat cgcgcttaca
      361 ccgatcagcg ccttgatgtc ataggtcaac gcctgtttcg ccgtggtgag cgcggcagta
      421 tccggtgctg attccgggca gggctcaacg ttcaacgtca gtacatcgag cgggccttcc
      481 ttggttagca cgatctgata gtgaggcgcg agagcgtgtt gcttgagcag cagttcttcg
      541 atctgcgtcg ggaatacgtt gacgccgcgc acgatcatca tgtcgtctga acggcccgtg
      601 atcttttcca tccgacgcat ggtgcgcgcg ctgcccggca agagacgagt cagatcgcgc
      661 gtccggtagc ggacgatcgg cagcgcttcc ttcgttagcg acgtgaatac cagctctcct
      721 agctcgccgt ccggcaacac ttcgccggtt tcggggtcga tgatctcggg atagaagtgg
      781 tcttcccaga tagtcgggcc atctttggtc tcgacgcatt ccgaagcgac gccaggtccc
      841 atcacctcag acagaccata gatgtcgaca gcgtcgatgc ccatgcgttt ttcgatcgcc
      901 tgacgcatgt cgttggtcca cggttccgca ccgaaaatac cgatgcgcag cgagcaattc
      961 gccggctcga tgccctgccg ttcgagctcg tcggcgatcg acaacatata gctcggcgtc
     1021 accatgatga tgtcgggccg aaagtcctga atcagttgta cttgtttttc ggtctgaccg
     1081 ccgccgaacg ggatcaccgt cagccccgcg cgctcggcgc cgtagtgtgc gcccaggccg
     1141 cctgtgaaca gaccatagcc gtagctcacg tgcaccttgt cgccgcgcct cgcacccgcg
     1201 gcacggatcg aacgtgcgac gagatttgcc cacgtatcga tatcgcgcgc cgtgtagccg
     1261 acaaccgtcg gcttacccgt cgtcccggac gacgcgtgaa tccgcgagac ctgttcctgc
     1321 ggcaccgcaa acatgccgaa cgggtagctg tcgcgcaggt ccttcttggt ggtgaagggg
     1381 aagcgcgcca ggtccgcgag ggtcttcact tcgctggggc ggacgccggc ttcatcgaat
     1441 ttgcgccgat agaccggcga attctcgtaa gcatggttca gcgaccattt gagccgttcg
     1501 agttggagcg cggcgagttc gtcgcggctg gctgtctcga tgggatcgag cgggagggcg
     1561 gtggtcatcg aatgtctcct tgctgcctca atgcttgggt cgggggtgcg gtgcgcttgc
     1621 gcgcaagaac cggggactcg ttgctatcta tggatgctct gccaatgtgt cgcggacgcg
     1681 ccgcgattct gctgcgaatc tggcgcgact ctgctgttca atccgttgtt caatcacccg
     1741 agggaatcag gttgcctttg atctgcgcag atttgccgcg aaacatcgcc acggtctcct
     1801 ctgctcgatt catgacgcga atatcgtaga tgcccgtgcg gccgctcagc gtttgctcga
     1861 ccgcttcggc cgtcaacata tcgccgccgt gtactggccg cagaaactcg atcgaacagc
     1921 cggctgcgac tgtgttgatg ttgtatgtgt tgcaggcgaa agcgaacgtc gaatcagcga
     1981 gtgtgaaaat caggccgcca tggcaaatct gatgaccgtt cagaaagtcg tcacgaacgg
     2041 ccatacgcag gcgtgcgtac cctggacgta cttctacaat ctcgagcccg agcgcccgac
     2101 tgcaggcgtc gttttcgtac atggcgaccg cggttgcgcg agcgagttcg tccggcgtca
     2161 tttgggcggg atggttcgat tgcgtcgaca tgtcagcggc cctcgaagcg cggcgcgcgt
     2221 ttttccacga atgcttgcac gccttccgcg taatcgaggg aagcgccgag ttcgcgctgc
     2281 agatcgcgtt cgagatcgag ctggtgatcc agggtttgcg tcgcacccac gcgcattgcc
     2341 tgcttgatca tggcgatggc gcgcgtgggt tgctgcgcca gttgtgccgc gagtttgctt
     2401 gccgtggcgg ccagctcggt gtcgtcaacc gtttgccaga tcagccccca gttttcggct
     2461 ttctccgcac tcagtttgtc gccggtgatg gcgagaccca aggcgcgcgc catgccgacg
     2521 cgttgcggca ggaaccaggt gccgcccgag tctggtacga gaccgatctt cacgaatgcc
     2581 tggatgaagc tggccgaacg agcagcaagc acgagatcgc aagccagcgc aagattggcg
     2641 ccggcgccgg ccgccgtgcc gttgactgcg gcaatcacgg gcaatggcag cgcttgcagg
     2701 cggcgaatca gcggattgaa atgcgtgtcg atcagttcgc ccagatcggt catcgcgccc
     2761 ggcgtgaagt cgagatcggc gagatcctgt cccgcgcaga agccacggcc cgcaccggtg
     2821 agcacgagcg cccgggcgcc ggatgcctcg
//
LOCUS       DATASH010000200         4505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4180199,
            whole genome shotgun sequence.
ACCESSION   DATASH010000200 DATASH010000000
VERSION     DATASH010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4180199"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..136)
                     /locus_tag="VHV99_05585"
     CDS             complement(<1..136)
                     /locus_tag="VHV99_05585"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX3634950.1"
                     /translation="MTTLPERIQIVLDETGIDQTELAEAAGVTKSTVSQWLSLAIKSL
                     K"
     gene            660..2543
                     /locus_tag="VHV99_05590"
     CDS             660..2543
                     /locus_tag="VHV99_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3634951.1"
                     /translation="MTRKKLDFRGQAFKDVLGFTFVHWAKQPWRIVTITALVLLSALA
                     DVLTPMFAGRLVDAIASGSAGDAVAWHAAITAFCVLSALGLGATLLRQGVFFNIIRLT
                     LKMMSEIAANAFHRVQRFSTDWHANSFAGSTVRKITRGMWALDLLNDTLLIALLPSLV
                     MLVGATVLLGWRWPMMGAVVGIGSVLYIAVTVAMSLGFVAPAARLANAWDTRMGGALA
                     DAVSCNGVVKAFGAEEREEVLLERVIGKWRHRTRRTWIRGTINGGMQGAMLVAIQAAI
                     LGAALLLWARGEASVGDITFALTMFFMLQGYLRDVGMHIRNLQRSVNDMEELVSLESQ
                     PLGIEDRPGAGPIAIGKGEIRFEHVTFHYGTKATPLYDNFSVRIAPGERVGLVGHSGS
                     GKTTFIKLIQRLYDISEGRITIDGQDIARVQQASLRSQIAIVQQEPVLFHRSLAENIA
                     YARPGASRTEIERAAKLASAHDFIAALPQGYDTLVGERGVKLSGGERQRVAIARAFLA
                     DAPILILDEATSSLDSESEVLIQQAMERLMMGRTTLVVAHRLSTVRALDRLLVLDKGK
                     VIEEGSHEALIRLENGLYRRLFERQALELIKGLGDSDSEVARRTHGAKANRLDDSSLL
                     VGN"
     gene            complement(2735..3199)
                     /locus_tag="VHV99_05595"
     CDS             complement(2735..3199)
                     /locus_tag="VHV99_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2214 family protein"
                     /protein_id="HEX3634952.1"
                     /translation="MLVRWLLAALHLLAYGFALASILRRTWALRRASVPAALRSVFRA
                     DTGWGISALVLIVTGLMRAFGGLEKGADYYLHEPLFHAKMTLLVLIVILEIPSMLALM
                     RWRACIKKGVPPDLRKARKYARFSVIQTVLLVLMVFAATGMARGIGLPAGVV"
     gene            complement(3351..4205)
                     /locus_tag="VHV99_05600"
     CDS             complement(3351..4205)
                     /locus_tag="VHV99_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634953.1"
                     /translation="MARTKETRANAAPGAGTIFALRAIGLVLLARWLFSMAQMDLGAS
                     LSAMVSSPWACINLVFLFLLIFLPGARAVVERPFHPLPQWLRQALRLFAFLGFLFALW
                     SVGAFAASAGWRRAAQAVAATNGWLLAAPALYAAVLWICRPRALWRTNIAARRFAIGR
                     YAVALDPATRTVVVWAERRKVGQYDARELSVRWAAGQATSSTPTPTPAVALGVAGEPA
                     ASAAPLGSVGAPALARSGFGRRPKIELLWDSPAAAGHNRQTVFRTALTTEGDRVAARA
                     LDTSLQQV"
BASE COUNT          867 a         1441 c         1410 g          787 t
ORIGIN      
        1 gcttcagcga ctttatggcg agtgacaacc attggctgac ggtgcttttg gtgacgccag
       61 cggcttcggc cagctcggtc tgatcgatgc cggtctcgtc caggacaatc tggatacgtt
      121 caggaagtgt ggtcatggaa caggaatcta aactatacac ggtttagata tcttttaatt
      181 tccagtttag ttttctgcac atacatggag gcagcaagcg agtcagggga catcgttcgc
      241 tatacttcgt gacgttttgt cggtggaccg ctgcacgctg cttgatgccc ctgcgccggc
      301 cgcatcgaag tcaacaccga aactccgcac gattgcgacc gccaaatact gctgatatag
      361 tcagcgttcg ggcattcgct ccgtctactt tcttcacctc aatcgactac agggaggcgt
      421 cacatgttcg cagtgcatat cgccgtccct gagatggtct cacgaccatt ctcgcgacct
      481 taaagttgtc gcgtcgtcgc tcacgcggtt ctgcctgaac aggagttccg cgcaatttcc
      541 agacagtccg tagtctcatt gccggcattg ttgtcaactc ggcctggcgc gcttgactgt
      601 ctcttctttc gcttctgact ggaccctcgg tgcgctcgcg cgccaccggg tcgtagcaaa
      661 tgaccagaaa aaaactcgac tttcgtggac aagccttcaa ggacgtcctc ggcttcacct
      721 tcgttcactg ggctaaacag ccctggcgca tcgtcacgat taccgcgctc gtgctgctct
      781 cggcattggc cgacgtgctc acaccgatgt tcgccggacg gctcgtcgac gcgatcgcgt
      841 ccggctctgc cggcgacgcc gtcgcgtggc acgctgcgat cacggccttc tgcgtgttga
      901 gcgcgcttgg gctcggcgcc acgttgcttc gccagggcgt gttcttcaac atcatccggc
      961 tgacgctcaa gatgatgagc gagatcgccg cgaacgcgtt tcatcgcgtg caacgctttt
     1021 caacagactg gcacgccaac agcttcgccg gatccaccgt gcgcaagatc acgcgcggca
     1081 tgtgggcgct cgatctgctc aacgacacgc tgctgatcgc gctgctcccg tcgctggtca
     1141 tgctggtcgg ggcaacggtg ctgctcggct ggcgctggcc aatgatgggt gcggtcgtcg
     1201 ggatcggctc ggtgctgtac atcgcggtga ccgtggccat gtcgctcggc ttcgttgcgc
     1261 ccgccgcccg cctcgctaac gcatgggaca cgcgcatggg cggcgccctc gccgacgccg
     1321 tcagttgcaa tggcgtggtg aaagcgttcg gcgccgaaga gcgcgaagaa gtgctgctcg
     1381 aacgcgtgat cggcaagtgg cgtcatcgca cgcgccgcac gtggatacgc ggcacgatca
     1441 acggcggcat gcaaggcgcc atgctggtgg cgattcaggc cgcgattctg ggcgccgcgc
     1501 tgcttttgtg ggcgcgcggc gaagcaagcg tcggcgacat cacgttcgcg ttgacgatgt
     1561 tcttcatgct gcaaggctat ctgcgcgacg tgggcatgca catccgcaac ctgcagcgtt
     1621 cggtcaacga catggaggaa ctggtgtcgc tggagagcca gccgctgggt attgaagacc
     1681 gtcccggcgc gggcccgatc gcaatcggca aaggcgaaat tcgtttcgag catgtcacct
     1741 tccactacgg tacgaaggcc acgccgctct acgacaactt ctccgtgcgc atcgcgccgg
     1801 gagaacgcgt cgggctggtc gggcactcgg gctcggggaa aacgaccttc atcaagctga
     1861 tccagcggct gtacgacatt tcggaaggcc ggatcacgat cgacggtcag gacatcgccc
     1921 gggtgcaaca ggcgtcgttg cgcagtcaga tcgcgatcgt tcagcaggag ccggtgctgt
     1981 ttcaccgctc gctggccgag aacatcgcct atgcacggcc cggtgcgagc cgcaccgaga
     2041 tcgaacgcgc cgcgaagctc gccagtgcgc acgacttcat cgcggcgctg ccccaaggct
     2101 acgacacgct ggtgggcgaa cgcggcgtca agctctcggg tggcgaacgt cagcgcgtgg
     2161 cgattgcgcg ggcgtttctc gcggacgcgc cgatcctgat tctggacgaa gcgacctcga
     2221 gcctcgacag cgaaagcgaa gtgctgatcc agcaggccat ggagcggttg atgatgggcc
     2281 gcacaacgct ggtggtggcg caccgccttt ccaccgtgcg ggcgctggac aggctgctgg
     2341 tgctggacaa gggcaaggtc atcgaggaag gcagccacga ggcgctgatc agactcgaga
     2401 acggcctgta tcggcggttg ttcgaacgtc aggcactgga gttgatcaag ggtttgggcg
     2461 attcggattc agaggtcgcg cgcaggaccc acggcgcgaa ggcgaatcgg ctcgacgatt
     2521 caagcttgct ggtcgggaat taaatcgctg taagcgtgcg ggacgtcatg cggaagccgc
     2581 cggcaaaagg ccgtttctgc tggcgccacg cacgccccgc ttcgttcacc cgcgcgaacg
     2641 cgcacgcggc atttctcgcc tgccgcgctt gacggaacgc catgcgttgc ggcgcgactc
     2701 aacgcgacga accggccggc gcttcaacgc acgactagac cacgcccgca ggcagtccga
     2761 tacctcgggc catgccggtc gccgcgaaca ccatcaacac cagcaacacc gtctggatca
     2821 cgctgaaacg cgcatacttg cgcgcttttc gcaaatcggg cgggacgccc ttcttgatac
     2881 aggcacgcca acgcatcagc gcaagcatcg acgggatttc gaggatgacg atcagcacga
     2941 gcagcgtcat cttggcgtga aagagcggct catggagata gtagtcggcg cccttttcca
     3001 agccaccaaa ggcgcgcatc agaccggtga cgatcagcac caacgccgaa atgccccagc
     3061 ccgtatcagc gcggaatacg gatcgcaacg cagcgggtac gctggcgcgc cgcagcgccc
     3121 aggtgcgccg gagaatcgac gcgagcgcaa agccataagc gagtagatga agggcggcaa
     3181 gcaaccagcg aaccagcatg gcgactcctg caaacgacgc aatatgatcg atcgatgtat
     3241 gtccactcgc ccactgagac gccgggcgtc acttcgtatt atcgactaca gcttacttta
     3301 cgatgcctgg ttagcctgcg gggtgtagcc gactgcacaa agcaagccac tcagacttgc
     3361 tgcaacgagg tgtcgagcgc acgtgcggca acccgatcgc cttcggtcgt gagcgcggtg
     3421 cggaacacgg tttgtcgatt gtggcctgcc gcagccggcg aatcccacag caattcgatt
     3481 ttcggacgtc ggccgaagcc gctgcgagcc aacgcgggcg cgccgaccga gccgagcggc
     3541 gcggcggatg cagcgggctc gcccgcaaca ccgagcgcca ccgcgggggt aggcgtcggc
     3601 gtcgaactcg ttgcttggcc cgccgcccat cgaacggaca attcgcgcgc gtcgtattgg
     3661 cctaccttgc gccgctcggc ccagacgacc accgtgcggg tggccggatc gagcgcgacc
     3721 gcatagcggc cgatcgcaaa gcgtcgcgcg gcgatattgg tccgccacag cgcgcgcggc
     3781 cggcaaatcc agagcaccgc cgcgtacaac gccggcgccg ccaacagcca gccgttggta
     3841 gccgccaccg cctgcgcggc gcgccgccaa ccggcgctcg ccgcaaatgc gccgaccgac
     3901 cagagcgcaa acagaaagcc gaggaaagca aacaggcgca gcgcttgccg caaccattgc
     3961 ggcagcggat gaaacggacg ctcaaccacg gcccgcgcgc ccggcagaaa gatcagcaga
     4021 aacagaaata ccaggttgat gcacgcccac ggcgaggaca ccatcgccga cagcgacgcg
     4081 cccaggtcca tctgcgccat cgaaaaaagc cagcgagcga ggagcacgag accgatggcg
     4141 cgcagcgcga aaatcgtacc ggcgccgggc gccgcgttcg cacgcgtctc tttcgttctc
     4201 gccaaggcat cctcctgtcg tcggcggcgc cgcagccaaa tttgcggcac ggccattata
     4261 gggatattac ggagattggc tcatgctgcc ggtgtccaaa ccgtactttc taccgttcac
     4321 gaggcaaaac gcggcgcgca tgcaacaacg ccccgagggc gagacgcacg cggggcgttg
     4381 ttggtgcagc cggaccatct gatccgccgg gggcgccgag ccaaaaggcg ccgccagcgc
     4441 tccagccaga cggccggctg cttttcctta cggcatttag ctggcattga cttcgcgcag
     4501 caccg
//
LOCUS       DATASH010000201         2335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_421142,
            whole genome shotgun sequence.
ACCESSION   DATASH010000201 DATASH010000000
VERSION     DATASH010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421142"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="VHV99_05605"
     CDS             complement(<1..673)
                     /locus_tag="VHV99_05605"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HEX3634954.1"
                     /translation="MISIAPLSSEPWGTLPGGDPVRLFTLRNAHGMKVAISDLGATLV
                     SWHAPDRAGRLGDILLGHDTAAEYVAATTYMGGLIGRWANRIADARFTLDGIEYTLDR
                     NEGTNLLHGGTQGFNRTMWDASEDNGALVMRLESPEGDAGFPGNVTVQVRYTLDDDGT
                     LTIAYEAITDAATPLNLTSHPYFNLTGRRGADIRGHVLSIDADRFFEVDASMIPCELA
                     EVAGNA"
     gene            complement(707..1924)
                     /locus_tag="VHV99_05610"
     CDS             complement(707..1924)
                     /locus_tag="VHV99_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HEX3634955.1"
                     /translation="MTRPQTPQTTHRIALLFNANKVYDREIITGIGNYLLSTRVAWDL
                     FLEEDFRCRLTGIERFDGDGIIADFDDPAVGEALRDCALPVVAIGSSFEDPAHYPPDL
                     PYIATDNSKLVSLAYTHLIGAGLEHFALYSLPQAQENRWAQQRELAFAHLRSADGYSA
                     EQIGTEIYRGLSTSAPSWNQATEQLTAWLSQLPKPVGIIAVTDARARHLLQACLIAGI
                     PVPEEVAIIGIDNDPLTRTLTRIPLSSVIQGTEEMGRTAAHLLHQMLHGARFPGRRIL
                     VPPVGINVLESTKHQPLASPYVMRARHFIRQYACQGIRTEQVADYVGVSRSSLEEYFR
                     RERQCTVHQEILRHKLDVAKALLAKRDASSAEVAIRCGFTSLQYMYAVFRRELGCTPR
                     EYQERANSTATPG"
BASE COUNT          465 a          696 c          726 g          448 t
ORIGIN      
        1 acgcgttgcc cgccacttcg gcaagctcgc aaggaatcat cgacgcatcc acttcgaaga
       61 accggtcagc gtcgatcgac aacacatgtc cgcggatatc ggcgccgcgg cgacccgtga
      121 ggttgaagta cggatgactc gtcaaattga gcggcgtcgc tgcgtccgtg atggcctcat
      181 aggcgatcgt caacgtgccg tcgtcgtcga gcgtatagcg tacttgcacc gtgacgttac
      241 ccggaaaccc ggcgtcacct tcgggcgatt ccagacgcat gactagtgcg ccattgtctt
      301 cgctggcgtc ccacatcgtg cgattaaaac cctgcgtgcc gccatgcagc agatttgtgc
      361 cttcgttgcg atcgagtgtg tactcgatgc cgtcgagcgt gaagcgcgca tcggcaatgc
      421 ggttcgccca ccggccgatc aatccgccca tgtaggttgt ggccgcaaca tattcggcgg
      481 ccgtgtcgtg gccgagcaga atgtcgccga gacgtcccgc gcggtccggc gcgtgccagg
      541 agaccagcgt cgcgcccagg tcgctaatgg cgactttcat gccgtgcgcg ttgcgcagtg
      601 tgaagagccg cacaggatcg ccgccgggca gcgtgcccca gggctcggaa gaaagcggtg
      661 caatgctgat catgtcgtcg gtcgagaaaa tagatgaagg gcatgctcag ccgggcgttg
      721 ccgtggaatt cgcgcgttcc tggtactcgc gcggcgtaca accgagttcg cggcgaaata
      781 ccgcgtacat gtattgcagc gacgtgaaac cacaccggat cgccacctcg gcgctcgacg
      841 catcgcgttt cgcgagcagc gctttcgcga catcgagttt gtgtcgcagg atttcctgat
      901 gcacggtgca ctgccgttcg cgccggaaat actcttcgag cgacgaacgc gacacaccga
      961 cgtaatcggc aacctgctcg gtgcgaatac cctggcaagc gtactggcgg atgaagtgcc
     1021 gcgcgcgcat cacatacgga ctcgctagcg gctgatgttt ggtcgattcg agcacgttga
     1081 tgccaaccgg cggcacgaga atgcggcgcc ccgggaaacg cgcgccatgc agcatttgat
     1141 gcagcaagtg agcggcggtg cgtcccattt cctcggtgcc ttgaatcacg gaggaaagcg
     1201 gaatgcgggt gagcgtgcgc gtgagcggat cgttgtcgat gccgatgatc gcgacttcct
     1261 cgggtactgg aatacccgcg atcagacagg cctgcagtag atgccgcgcg cgggcgtcgg
     1321 taacggcaat gatgccgacc ggcttgggca attgactcaa ccacgcggtg agctgctcgg
     1381 ttgcctgatt ccacgacggc gcgctggtcg acagaccacg atagatctcg gtgccgatct
     1441 gctcggcgct gtaaccgtct gcgctacgca ggtgagcgaa tgccaactca cgttgctgcg
     1501 cccagcgatt ttcttgcgct tgcggcaggc tatacagcgc gaagtgttcg agccccgcgc
     1561 cgatcagatg cgtataggca agcgaaacaa gcttgctgtt atcggtcgcg atatagggca
     1621 agtccggagg atagtgcgcc ggatcttcaa acgatgaacc gatcgccacc accggcagcg
     1681 cgcaatcacg cagcgcttcg cccacagcgg gatcgtcgaa atccgcaatg atgccgtcgc
     1741 cgtcgaaacg ctcgataccc gtcaaccggc agcggaagtc ttcttcgagg aagagatccc
     1801 acgccacgcg cgtcgacagc aggtaattgc cgatgccggt gatgatctcg cggtcataca
     1861 ccttgttcgc gttgaacagc agcgcaatcc gatgggtcgt ctgtggtgtt tgcggacggg
     1921 tcatggcagt gggcggcgtg cggatcgtca ggactcgcgg cgccttcgtc tcctgtaggg
     1981 tgcgcgccga acgcggcacc gtttttaagc gtctggttat tctaggccgc gaagttcgcg
     2041 cctgtgtgaa acaaacgcac gggcagtaag aaacgttatg cacgctgcgc aatttcgcaa
     2101 ttgccgttgc ctccagcaag cggcagcatg gcgtcacctt atcgaccggc acggcgatgt
     2161 cgcacgggtt gcagtgcagc ataggatttg agcgagcgct aaagcgctaa ctcaggtcga
     2221 cacacaggag acgctgatgt cctacttcga acacattccc gagatccgct atgaaggccc
     2281 gcaatcggat aacccgcttg cgtatcgcca ttacgacaag agcaaacgcg tgctc
//
LOCUS       DATASH010000202         9125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_618795,
            whole genome shotgun sequence.
ACCESSION   DATASH010000202 DATASH010000000
VERSION     DATASH010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 9125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9125
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618795"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..676)
                     /gene="moaC"
                     /locus_tag="VHV99_05615"
     CDS             complement(197..676)
                     /gene="moaC"
                     /locus_tag="VHV99_05615"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEX3634956.1"
                     /translation="MPELTHFDAAGQAHMVDVGSKQETKRIAIARGSIRMLPETFALI
                     RDGNAKKGDVIGIARIAAIQGSKRTADLIPLCHPLALTRVKVDFELDETLPGVHCTVQ
                     VETLGRTGVEMEALTAVQVGLLTVYDMCKAVDRGMTITDVKVMEKHGGKSGDWVADS"
     gene            810..2543
                     /locus_tag="VHV99_05620"
     CDS             810..2543
                     /locus_tag="VHV99_05620"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX3634957.1"
                     /translation="MRSKRFLAALLCFALATPPGAYAQSRGVSGTSTNTSTSLALNMQ
                     SAEPPLESGPAETLANPIIPPDIAQGVFGVYGGAQSRFSGNSGVTTNWRAPIVPQQLP
                     DLGNGGSDSLTPQAERRLGERVMRELRRDPDYLDDWLMRDYLNSVAAKLAAAANALYI
                     GGYRPDFELFAVRDAQINAFSLPGGFIGVNTGLIVTTQTESELASVLGHEMGHVLQRH
                     ISRMITTGERSSYAALAGLLFGILAGVLAHSGDLGSAIAIGGQAYAVDNQLRFSRSAE
                     HEADRVGFLLLAGAGYDPYAMTTFFGRLDRAAMSDTGIPAYARTHPLTGERIADMEDR
                     ARRAPYRQPHQTSEYGFVRARARLLQDRSRSEYADEISRLRSEIEDRTAVNVAANWYG
                     IAYGQMLLERYDDAVASLAMSRAAFATGERSEGEPTRSSPSLDVLAADIARRAGRDDE
                     AARLADFAQKRWPQSNAAIDMHIQTLLTLKRFAAAQALALKETRAQPDQPAWWLYLAQ
                     ASAGSGDALTQHRALAEKFALEGAWPSAIRQLKEARDLKAIGYYDLATVDARLHEMES
                     RYKEERLDEKS"
     gene            complement(2617..3195)
                     /locus_tag="VHV99_05625"
     CDS             complement(2617..3195)
                     /locus_tag="VHV99_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HEX3634958.1"
                     /translation="MDDIVKQALAKWPNVPSCTGWLMLDRRGNWRMRDEAAQAGGEPG
                     TAIRHEALLGFINRNYDADERGQWFFQNGPQRVYVELGYTPWVVRLSADAAGALALTD
                     QAGGAFEPSAVFVDDEGGILFVDSSAPPRVAVLHDHDLEVFSDHAALGDDSMSGEFRW
                     NKGVVLPLQGVLRNEVAERFGFVDSPAAAASA"
     gene            complement(3188..4327)
                     /locus_tag="VHV99_05630"
     CDS             complement(3188..4327)
                     /locus_tag="VHV99_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX3634959.1"
                     /translation="MSTPRRKSPAPNEAPSGAWGTTHDESASSGAARAVAAVEATVDL
                     LYRAPLWLPNRHVQTIVPSLFARRPAIAFRRERWDTPDGDFIDLDWVVHNGQTDAARA
                     PASPPHVPADDAPLFVLFHGLEGSSTSHYAATLMAAAREYGWHGVVPHFRSCSGPLNL
                     LPRFYHLADSNEVDWILRRLRATHRGPIVAAGVSLGGNVLLRWLGERREEASPVLSAA
                     AAISTPIDVHAGGRALSQGFGMVYTRNFLKTLKQKAEQKLAQFPGLFDRDAMLASRTM
                     YDFDNVVTAPLHGFRDTDHYWSSATTRPLLPGIAVPTLVLNARNDPFLPAEALPARHQ
                     VSATVELDQPRHGGHAGFMTGPFPGRIDWLSRRVLGYLERYVDHG"
     gene            complement(4395..4841)
                     /locus_tag="VHV99_05635"
     CDS             complement(4395..4841)
                     /locus_tag="VHV99_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010090801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX3634960.1"
                     /translation="MPRFAHIFEAAADTLNAYYQAVAEVNIDSLMGLWIDEEFVSCIC
                     ADGSHLHGLESIRAGLQIQLEAAPVSIEPLDIRVYDSLGTVVYAIAEAHRPADPKATP
                     AMVFTTYVMVHERGEWKIAHIHASPMPNEAASQFATKMRHGQGSLH"
     gene            complement(5020..6045)
                     /gene="waaF"
                     /locus_tag="VHV99_05640"
     CDS             complement(5020..6045)
                     /gene="waaF"
                     /locus_tag="VHV99_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011401829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEX3634961.1"
                     /translation="MRRALVIAPNWIGDALMAQPLFARLVKLHPRIVIDAVAPSWVAP
                     VLERMPEIRDVYATDLAHGKLQMLRRWQLASDLRDVGYDAAYVLPNSFKSALIPWMAG
                     IPLRIGYTGESRYGLLNVRHTTPRKDERPPMVGQYAALAYAPGAKLPGDLPTPRLDAD
                     LNEASRVSARFNLDTRVPLLVFCPGAEYGPAKRWPPEYFAALAQMVGQSFPYTQIVAL
                     GSPKDAPLAQAIAEKAPNVRNLCGQTALGEACALIARANAVVTNDSGLMHVAAALRRP
                     LVAVYGSTDPRHTPPLSELAKVQWLHLECSPCFQRECPLGHLNCLKQLSAEQVFGDLR
                     GMLLAQR"
     gene            complement(6306..6500)
                     /locus_tag="VHV99_05645"
     CDS             complement(6306..6500)
                     /locus_tag="VHV99_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEX3634962.1"
                     /translation="MSEIKEMPLVELSAKDLPAYCPNPSMPRWSTHPRVFIDVTHGEA
                     RCPYCGTRYKLRDGEVVRGH"
     gene            complement(6594..7517)
                     /locus_tag="VHV99_05650"
     CDS             complement(6594..7517)
                     /locus_tag="VHV99_05650"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEX3634963.1"
                     /translation="MSMADRDGKIWMDGKLIDWRDAKIHVLTHTLHYGMGVFEGVRAY
                     KTADGGTAIFRLQEHTKRLLNSAKIFQMDVPFDHETLAAAQCEVVRENKLESCYLRPI
                     IWVGSEKLGVSAKGNTIHVAIAAWPWGAYLGEDGIAKGIRVKTSSFTRHHVNVSMVRA
                     KASGWYVNSILANQEAIADGYDEALLLDVDGYVSEGSGENFFLVNNGKLYTPDLSSCL
                     DGITRDTVITLARDAGIQVIEKRITRDEVYTCDEAFFTGTAAEVTPIRELDNRTIGSG
                     ARGPITEKLQSGFFDIVNGKSEKYAHWLTKI"
     gene            7826..8572
                     /locus_tag="VHV99_05655"
     CDS             7826..8572
                     /locus_tag="VHV99_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEX3634964.1"
                     /translation="MLARLSDTDRRAFREGARAYSPTLMAIFSWGLVTGIAMSKSVLT
                     LPQSLTMSLLCYAGSSQLAVLPLLAARLPIWTVLLTAAMVNTRFVIFSAGLAPHFSYL
                     PMWRRIVLGYFNGDVIYLLFQKKNFQTGYVPGKEAYFWGMAVCSWLSWQVSSIVGILL
                     ASLIPDNWGLALAGTLALLPIMVSAIATRSTLVAVSMAGVVSLLAFDLPYRLALPLAV
                     IAAIIAGSGADLMAERADLRRIRNSAGDSQ"
     gene            8569..8892
                     /locus_tag="VHV99_05660"
     CDS             8569..8892
                     /locus_tag="VHV99_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006763506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEX3634965.1"
                     /translation="MTSTQIWLAIIGMTFVTALTRAMFLIGGERTVLPARVQRMLRYA
                     PAAALAAVVLPDVLATPEGLSFAPSNHEFYATLSGLAWFLWRRTMLGTIVVGMVVFTL
                     LRLFV"
BASE COUNT         1628 a         2899 c         2912 g         1686 t
ORIGIN      
        1 cccgcacggc aaacgcgtgc ttgccacttg ttacttcttt tcctgatcga tgccatttgc
       61 tgtctccaac ttggacagaa tttggacaag atcgattttt cgtatgccag aaggcagatt
      121 tgtagtgatt ttgtcaggcg atggcttcat tgcagaatca agcgaagcgt caaattaata
      181 cttataaaac aatgccttaa ctgtccgcca cccaatcccc cgacttcccg ccatgcttct
      241 ccatcacctt cacatcggtg atcgtcatcc cccgatccac cgccttgcac atgtcgtaca
      301 ccgtcagcag cccgacttgc acggcggtta gcgcttccat ctccacgccc gtgcgtccga
      361 gcgtttcgac ctggaccgtg caatgcacgc caggcagcgt ttcatcgagt tcgaagtcca
      421 ccttgacgcg tgtcagcgcc agcggatgac atagcggaat cagatcggcg gtgcgcttcg
      481 agccttgaat cgcggcgatg cgcgcgatac cgattacgtc gcccttcttg gcgttgccat
      541 cgcgaatcag cgcgaaggtt tccggcagca tgcgaatcga gccacgcgcg atagcgatgc
      601 gcttggtctc ctgcttgctg ccgacgtcca ccatatgcgc ctggccggcg gcgtcgaaat
      661 gagtgagttc aggcatggtc gggctccttc agagggcgcc tatcatagca gtatggccgg
      721 gggagccaag atgattacaa ttgccctatg cccgctatct tagtccgagc ggcgggcgtc
      781 gcttttcgac ttcgctaacc gacctcgcga tgcgttcgaa acggtttctt gctgcgttgt
      841 tgtgtttcgc gctcgcgact ccgccgggcg cgtacgcgca atcgcgcggc gtatcgggca
      901 cgagtacgaa tacaagcaca agcctcgccc tcaacatgca gtccgcggag ccgccattgg
      961 aaagcggccc agccgaaact ctcgccaacc ccatcatccc gcccgatatc gcgcaaggcg
     1021 tcttcggcgt ctatggcggc gcccagagcc gcttctccgg caactcgggc gtgaccacca
     1081 actggcgcgc cccaatcgtc ccgcagcaat tacccgacct cggcaatggc ggctccgact
     1141 cgctgacgcc gcaagccgag cgcaggctcg gcgagcgcgt gatgcgcgaa ttgcgccgcg
     1201 acccggacta tctcgacgac tggctgatgc gggactacct gaactcggtc gccgcgaaac
     1261 tcgccgcggc ggcgaacgcg ctatatatcg gcggctatcg tccggacttc gagttgttcg
     1321 cagtccgcga tgcgcagatc aacgcgttct cactgccagg tggctttatt ggcgtaaaca
     1381 ccggtttgat cgtgacgacg caaaccgagt ccgaactcgc ctccgtgctc ggccacgaga
     1441 tggggcacgt cttgcagcgg cacatttcgc ggatgatcac gaccggcgag cgcagctcct
     1501 atgccgcgct cgccggcctg ttgttcggca ttctggcggg cgtgctcgcg cacagtggcg
     1561 acctcggcag cgcgattgcg attggcggtc aggcgtatgc ggtcgacaac cagctgcgtt
     1621 tctcgcgctc cgccgaacat gaagcggacc gcgtcggctt tctgctgcta gccggcgccg
     1681 gctacgaccc ctacgcgatg acgacgtttt ttggccgcct cgatcgcgcg gcgatgagcg
     1741 acacgggcat cccggcctat gcgcgcaccc atccgctgac tggcgaacgg attgccgaca
     1801 tggaggaccg tgcgcgccgc gcgccttacc ggcaaccgca tcagacgtcg gaatacggtt
     1861 ttgtgcgagc gcgggcgcgc ttgctgcagg accgctcgcg cagtgaatac gcggacgaga
     1921 tttcgcgcct gcgctccgaa atcgaggacc gtaccgcagt gaacgtggcc gcgaactggt
     1981 acgggattgc atacgggcag atgctgctcg agcgatacga cgatgcagtg gcgtcgctgg
     2041 ccatgtcgcg tgccgcgttc gcaaccggcg agcgcagtga aggtgagccc acgcgcagtt
     2101 cgccgagcct cgacgtgctg gcggccgaca tcgcgcgccg cgccgggcgt gatgacgagg
     2161 cggctcgcct ggccgacttt gcacagaagc gctggccgca gtcgaacgcc gcgatcgaca
     2221 tgcatatcca gaccttgctg acgctgaagc gcttcgccgc tgcgcaggct ttggctttga
     2281 aagagacgcg tgcgcagccc gatcagcctg cgtggtggtt gtatctggcg caggcgagtg
     2341 cgggcagcgg ggatgcgttg acgcagcatc gggcgctcgc cgaaaaattc gccctcgagg
     2401 gggcatggcc gtcggccatc cggcaactga aggaggcgcg cgatctgaag gcgatcggct
     2461 actacgatct cgccaccgtc gatgcccggt tacatgaaat ggagagccgg tataaggagg
     2521 aacggctcga cgagaagagt taggactcgc ccgccttcgc gatctgacca gaacgcggac
     2581 tatgcagcga atcccagcaa tcagtagcca aacgacctaa gcactcgccg cagcggccgg
     2641 actatccaca aatccaaacc gttcggcaac ctcatttcgc agcacgccct gcaaaggcaa
     2701 aacaaccccc ttgttccagc gaaactcccc gctcatggag tcgtcgccca gcgccgcgtg
     2761 atcggaaaaa acctcgagat cgtgatcgtg caaaacggcg actctcggtg gcgccgagct
     2821 atcgacaaac agaatcccgc cttcatcgtc gacgaacaca gccgaaggtt cgaaagctcc
     2881 gccggcctgg tcagtcaagg caagcgcgcc agcggcatcg gccgacaacc tgacgaccca
     2941 cggcgtatag cccaactcga catacacccg ctgcggccca ttctggaaaa accactgccc
     3001 gcgttcgtcg gcgtcgtaat tccgattgat aaacccgagc aacgcctcgt gccggatcgc
     3061 cgtgcctggc tcgccgccgg cctgagcggc ttcgtcccgc atgcgccagt tgccgcgccg
     3121 gtccagcatc aaccagccgg tgcaactcgg cacattcggc catttggcca aggcctgctt
     3181 gacaatgtca tccatgatcg acgtaccgct ccagataacc gagcacgcgc cgcgacaacc
     3241 agtcaatgcg ccccgggaag ggcccggtca taaaaccggc gtggccgcca tgcctgggtt
     3301 gatcaagttc caccgttgcc gacacctggt gccgcgccgg caacgcttcg gccggtaaga
     3361 acgggtcgtt gcgtgcgttc agtacgagtg tcggcacggc aatgccgggc agcaagggcc
     3421 gcgtggtcgc gctgctccag tagtggtcgg tgtcgcgaaa gccgtgcagc ggtgccgtca
     3481 ccacgttgtc gaagtcgtac atcgtgcggc tcgccagcat cgcgtcgcgg tcgaacagcc
     3541 cgggaaactg cgcgagcttc tgctcggcct tctgcttcag cgtcttcagg aaattgcgcg
     3601 tgtagaccat gccgaagccc tgcgacaacg cccggccgcc ggcatgcacg tcgatcggcg
     3661 tcgaaatcgc cgcggcggcc gagagcaccg gcgacgcctc ttcgcgccgc tcgccaagcc
     3721 agcgcagcag cacattgccg ccgagcgaca cgcccgccgc cacgatcggc ccgcgatgcg
     3781 tggcgcgcaa gcgccgcaga atccagtcga cttcgttgct atcggcgaga tggtagaagc
     3841 gcggcaacag attcagcggt ccgctgcaac tgcgaaagtg cggcaccacg ccgtgccagc
     3901 cgtactcacg cgcggccgcc atcaacgtgg ccgcgtagtg cgacgtggaa ctgccttcga
     3961 ggccgtgaaa caggacgaat agcggagcat cgtcggcggg aacgtgaggc ggggaggcgg
     4021 gggcgcgcgc agcgtcggtt tggccgttat gcacgaccca atcgagatcg atgaaatcgc
     4081 catccggcgt atcccagcgt tcgcgccgga acgcaatggc cggacggcgc gcaaacagcg
     4141 atgggacaat ggtctgcaca tgccggttcg gcagccagag cggcgcgcgg tagagcagat
     4201 ccaccgtagc ctcgactgct gccacggcgc gtgctgcgcc gctagaagcg gattcatcgt
     4261 gcgtcgttcc ccaagccccc gagggggctt cgttcggggc gggcgacttc ctgcggggcg
     4321 tgctcatgcc agcgtcgaag caaaaaagta aaaccggtaa aacggtctga acagacgaaa
     4381 acgcccgtca ggcgtcagtg cagcgaaccc tggccatgcc gcatcttcgt ggcgaactga
     4441 ctggcggcct cattgggcat cggactcgcg tgaatatggg caatcttcca ctcgccgcgt
     4501 tcgtggacca ttacatacgt ggtgaacacc atcgcgggcg tcgccttcgg atcggccggg
     4561 cggtgtgctt cggcaatggc atagacgact gtgccgaggc tgtcgtagac gcgaatatcg
     4621 agcggttcaa tcgaaacggg cgcggcttcg agctggattt gcaagccggc ccgaatgctc
     4681 tcgagaccgt gcaggtggga gccgtcggca cagatgcagc tcacgaactc ctcatcgatc
     4741 cacaggccca tcaagctgtc gatattcacc tcggcgacgg cctggtaata ggcgttcagc
     4801 gtatcggcgg cggcttcgaa gatatgggca aaacgtggca tggctcgtca gtctcttgtg
     4861 cgcggcgcgc ggttgcgggg cagcggtcag cgggacagtc cggcgcaccg gacaatcgca
     4921 cccaagcgcg agaatgcccg tgccgcaagg cgcgaacatg acgcgacgcg ctcaggggcg
     4981 tgcggtgttg cgtgcaagta tcggtcgaat tagctgggtt caacgttgcg caagcaacat
     5041 gccgcgcaaa tcgccgaata cctgctcggc actcagctgc ttcaggcagt tcagatggcc
     5101 gagcggacac tcgcgctgaa aacaggggct gcattcgaga tgaagccatt gtaccttcgc
     5161 aagctcggac aagggcggcg tgtggcgcgg atcggtcgaa ccgtacacgg ccaccagcgg
     5221 ccggcgcagc gcggcggcca catgcatcag gccggaatcg ttggtgacga ccgcgttggc
     5281 tcgcgcgatc agcgcgcaag cctcgcccag cgccgtttgg ccgcaaaggt tgcgcacgtt
     5341 cggcgctttc tcggcgatcg cctgcgcgag cggcgcgtct ttcggtgaac cgagcgcgac
     5401 gatctgtgta tacgggaacg actgccccac catctgcgcg agcgccgcga aatactcggg
     5461 cggccagcgt tttgccggac cgtactcggc gcccgggcag aacaccagca gcgggacgcg
     5521 cgtgtccaga ttgaagcgcg ccgacacgcg cgacgcttcg ttcaggtccg cgtcgagccg
     5581 gggtgtcggc aggtcgccgg ggagcttggc gccgggcgca taggcgaggg ctgcgtactg
     5641 accgaccatg ggcgggcgct cgtccttgcg cggggtcgtg tgccgcacgt tcagcaagcc
     5701 gtagcggctt tcgccggtgt agccgatgcg cagcggaatg cccgccatcc acggaatcag
     5761 cgccgacttg aacgaattcg gcaggacata ggccgcgtcg tagccgacat cgcgcaagtc
     5821 gctcgccagc tgccagcggc gcagcatctg cagcttgccg tgcgcgaggt ccgtagcgta
     5881 gacgtcgcga atctcgggca tgcgttcgag cacgggcgca acccacgaag gcgcgaccgc
     5941 atcgatcacg atgcgcggat gcaatttcac gaggcgcgca aacagcggct gcgccatcaa
     6001 tgcgtcaccg atccagttcg gtgcgataac caacgcgcga cgcatcagag tgagtatccg
     6061 ggttcgaagc gaagcgccgc gcgacccatg cgcgtggcgt tcgattgttg aaaaggggaa
     6121 aagccgcgag ctttgcggcg cggttccagc atggccggcc ccgccgcggc tgcggcctgg
     6181 ttgcgaagcg cctggcttgc tacgctctgc tgcaaaacca gaaccgatcc gtcaaacaac
     6241 ggaccaggca gcaggcacgc aggcaagaag cgcagcaagc gatcaacgcg cagtgttcag
     6301 caggcttaat gacccctgac cacttcgccg tcgcgcagct tgtagcgcgt gccgcaatac
     6361 gggcagcgcg cttcgccgtg cgtgacgtcg ataaagacgc gcgggtgcgt gctccagcgc
     6421 ggcatcgacg gattcggaca atatgccggc agatcttttg ccgacagttc gaccagcggc
     6481 atttccttga tttcgctcat ggggactgtc tcgttgtatg cagggtccgt gcgttgcgcc
     6541 gtcaaaaggg cgatggccga ggggccgagt tcctggtggc ggcgcagcgc gcgttagatc
     6601 ttggtcagcc agtgcgcgta tttctcgctc ttgccgttca cgatatcgaa gaagcccgat
     6661 tgcagcttct cggtgatcgg gccgcgtgcg cccgagccga tcgtgcggtt gtcgagttcg
     6721 cggatcggcg tgacttcggc ggcggtgccg gtgaagaacg cttcgtcgca ggtatagacc
     6781 tcgtcgcgcg tgatgcgctt ttcgatcacc tggatgcccg cgtcgcgcgc cagcgtgatg
     6841 accgtgtcgc gcgtgatgcc gtcgaggcac gacgacagat ccggcgtata gagcttgccg
     6901 ttgttcacga ggaagaagtt ctcgcccgaa ccttccgaca cgtaaccgtc cacgtcgagc
     6961 aagagcgctt cgtcgtaacc gtcggcgatg gcttcctgat tcgcgaggat cgagttcacg
     7021 taccagcccg acgccttggc gcggaccatc gacacgttca cgtgatggcg cgtgaacgac
     7081 gaagtcttca cgcggatgcc cttggcgata ccgtcttcac cgaggtaagc gccccacggc
     7141 caggcggcga tcgccacgtg gatcgtgttg cccttggcgg acacgccgag cttttccgag
     7201 ccgacccaga tgatcgggcg caggtagcag gattccagct tgttttcgcg caccacttcg
     7261 cactgtgcgg cggccagcgt ttcgtggtcg aacggcacgt ccatctggaa gatcttggcc
     7321 gagttcagga ggcgcttggt gtgttcctgg aggcggaaaa tcgcggtgcc gccatccgcc
     7381 gtcttgtagg cgcgtacgcc ttcgaacacg cccatgccgt aatgcagcgt gtgagtgagc
     7441 acgtggatct tggcgtcgcg ccagtcgatg agcttgccat ccatccagat cttgccgtcg
     7501 cggtcggcca ttgacatacg attctccagc gggaatgtgt gagagccccc aaacctgccg
     7561 ctgcgcgtcg agtcccccga gggggacatc cccaagggga cttccttcgg gcggaaaacg
     7621 cggggcggcc cggggttttc ttttggtgtg aggtcgagga gcgctatttt agcgtctttt
     7681 gcacaaggaa cgggcatttg gccgcatcgc gggcgctata cttcagcgca cgcataattc
     7741 aaatccggat gcgccagcgg gtgcctgacg acctggcgtc gacctccctt cggtgcggcg
     7801 ccccttggcg cccgcgtttc acctcatgct cgctcgtcta tcagataccg atcgtcgtgc
     7861 tttccgtgag ggcgcgcgcg cctattcgcc cacgctgatg gcgattttct cctggggcct
     7921 ggtcacgggt attgcgatga gcaagtccgt acttacgctg ccgcagtcgc tcaccatgtc
     7981 gttgctgtgc tacgcgggtt cgtcgcaact ggcggtgttg ccgctgctcg ccgccagatt
     8041 gccgatctgg accgtgctgc tcacggccgc gatggtcaat acgcgtttcg tgatcttcag
     8101 cgcaggcctc gcgccgcatt tttcgtatct gccgatgtgg cggcgcatcg tactcggcta
     8161 cttcaacggc gacgtgatct atctgctgtt ccagaagaag aacttccaga ccggctacgt
     8221 gccgggcaag gaggcgtact tctggggcat ggccgtgtgc agctggctgt catggcaggt
     8281 gtcgtcgatc gtcggcattc tgctggcgag cctgattccg gataactggg gtctcgcgct
     8341 ggcgggcacg ctggctttgc tgcccatcat ggtgtcggcg atcgccaccc gttccacgct
     8401 ggttgccgtc agcatggctg gcgtggtgtc gctgctggcg ttcgacctgc cgtaccggct
     8461 cgcgctgccg ctggcggtga tcgccgcgat catcgccggc agtggcgccg acctgatggc
     8521 cgaacgcgcc gacttgcgcc gcatccgtaa cagcgccgga gattcccaat gacatccacg
     8581 cagatctggc tcgccattat cggcatgacc ttcgtcaccg ccctgacacg cgcgatgttt
     8641 ctaatcggcg gcgagcgcac ggtgttgccg gcgcgcgtgc agcggatgct gcgctatgcg
     8701 ccggccgcgg cgcttgccgc cgtggtgctg cccgatgtgc tggcgacgcc ggagggactc
     8761 tcattcgcgc cgtcgaacca cgagttttac gcgacgctgt ccggtctggc gtggtttctg
     8821 tggcggcgca ccatgctcgg cacaattgtg gtgggaatgg tcgtgttcac actcctgcgc
     8881 ctcttcgtgt gacggatcga ggcgaaaatg ctgcattgcg gaatgcgtgg cgcatcctcc
     8941 aaatacggga cattcgccta gttgcgggtc agaaggcagt gtggatcagt taaaatggtc
     9001 gtttccggga taagcgcgcc ggtgcatggc gcgacagccg cggccgagcc gctggcgccc
     9061 gattcagtgg agccgccgcc gtgccgaagc gctggagttt gccgcgcctc tatggcagtg
     9121 cgtcg
//
LOCUS       DATASH010000203         4308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4772242,
            whole genome shotgun sequence.
ACCESSION   DATASH010000203 DATASH010000000
VERSION     DATASH010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4308
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4772242"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..612)
                     /gene="efp"
                     /locus_tag="VHV99_05665"
     CDS             complement(55..612)
                     /gene="efp"
                     /locus_tag="VHV99_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEX3634966.1"
                     /translation="MKTAQELRVGNVVMIGSDAMVVLKAEYNKSGRNSAVVKMKFRNL
                     LSTGNMESVYKADDKFDVVQLDRKEVTYSYFADPMYVFMDADYNQFEVEADMMGDALH
                     YLADGMPCEVVFYNEKAISVELPTTLVREIIYTEPAVKGDTSSGKVLKNAKLNTGFEL
                     QVPLFCNIGDKIEIDTRTHEYRSRA"
     gene            complement(844..2046)
                     /gene="earP"
                     /locus_tag="VHV99_05670"
     CDS             complement(844..2046)
                     /gene="earP"
                     /locus_tag="VHV99_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HEX3634967.1"
                     /translation="MSSSSEQLPAASGHASVDTGAPAINIACDIFCAVIDNFGDIGVC
                     WRLARQLASEHDWQVRLFVDDLKAFHALCPAVDPALDRQSAAGVMIEHWHAPSHAGDT
                     LQIADVVIEAFGCELPAVYVSAMARREKKPVWVNLEYLSAEDWVADFHLRPSPHPRYA
                     LDKTFFFPGLGPGTGGVLKERLLDAGRDAFDRDAWWHARVGIAPPPSGTTVISMFAYE
                     NPAIDSLLEQWRDCATPIALLVPEGRISGAIARFFDLPDFRAGAKAKSGSLSAYALGF
                     VEQPLFDALLWAADLNFVRGEDSFVRAQWAQKPFVWHIYPQGDDAHLPKLDAALAHYA
                     NSLPDGARASLERFWHAWNGAGAPDWVDFWQNRALLERRAADWATELARVGDLAGNLA
                     EFAKTQLK"
     gene            2314..>4308
                     /gene="uvrC"
                     /locus_tag="VHV99_05675"
     CDS             2314..>4308
                     /gene="uvrC"
                     /locus_tag="VHV99_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEX3634968.1"
                     /translation="MTDSDVFDPKKSLAQLPHLPGVYRYYDEHGNVLYVGKARDLRKR
                     VSSYFTKTLLSPRIAMMVTRIRKIETTVTRSEAEALLLENNLIKALAPRYNILFRDDK
                     SYPYLKLTGHKFPRMAYYRGGVDKKNQYFGPFPSAWAVRESIQILQRVFQLRTCEDSV
                     FTNRTRPCLLHQIGRCTGPCVGLINEEDYARDVSNASRFLLGRQGEVMKELEQKMHAF
                     AADLKFEQAAAVRNQMSSLSTVLHQQAIEVGGDNDVDILAVVALGGKVCVNLAMVRGG
                     RHLGDKAYFPTHVEAALAIEDAMPEELAGDDFVDGVANIVTNIVIEQVDADVDALVDE
                     LALPAGTLAAREQDFEAHATTGEMEDEIAETGAAPLNEATELERQGALEAEVLEAFIA
                     QHYIGNRVPPVLVVSHAPTNRELVDLLIEQAGHKVVVLRQPQGQKRAWLSMAESNAKL
                     ALARLLSEQGSQQARTRSLADTLSLEMDDLAQLRIECFDISHTMGEATQASCVVYHHH
                     KMQSAEYRRYNITGITPGDDYAAMRQVLTRRYEKMLAQAAARASEANDEPQQEAAGLE
                     AEAPLEPTRPAEADVAEPVPSGGCLPNIVLIDGGKGQVEIARQVFAELGLDRSMLVGV
                     AKGEGRKVGLETLIFADGRAALELGKESAALMLVAQIRDEA"
BASE COUNT          870 a         1275 c         1332 g          831 t
ORIGIN      
        1 catttttttc tcagtgccac gtttgcggtg acacgggatc aaatgggccg gcgcttacgc
       61 gcggctgcgg tactcatgcg tacgcgtgtc gatctcgatc ttgtcgccga tgttgcagaa
      121 gagcggcact tgcagttcga agccggtgtt cagcttggcg ttcttcaaca ccttgcccga
      181 cgacgtgtca cccttgacag ccggttccgt gtagatgatc tcgcggacca gcgtggtcgg
      241 cagttccacg gagatggctt tctcgttgta gaacaccact tcgcacggca tgccgtctgc
      301 gaggtagtgc agggcgtcgc ccatcatgtc ggcttcgact tcgaactggt tgtagtcggc
      361 gtccatgaac acgtacatgg ggtcagcaaa gtacgagtac gtcacttcct tgcggtcgag
      421 ctggacgacg tcgaacttgt cgtccgcctt gtacaccgat tccatgttgc cggtggaaag
      481 caggttacgg aacttcatct tcacgaccgc ggaattgcgg ccggatttgt tgtactcggc
      541 cttcagcacg accatggcat cgctgccgat catcacgacg ttgccgacgc ggagttcttg
      601 tgcggtcttc atattaaaaa actgtcctgt acgaaaatgt gagcctgatc gctgcagtgt
      661 cgacggccgg ctcgtggcag ggttgcttgc tttaatcgaa atcaaaacgc aaacccgggc
      721 gctgtagccg cctcactgac tattcattga ctattcccac cgtgcgtgct ccactgtgta
      781 ttccactgtg gaattcgtcc ggaactgatg gcagttccgg actgcaagcc gttggataac
      841 cgcttatttt aactgagttt ttgcgaattc cgccagattt ccagccagat cgccaacgcg
      901 cgccagttcc gtcgcccaat cggcggcgcg ccgttccagc agcgctcggt tttgccagaa
      961 atcgacccag tccggagcgc ccgcgccgtt ccacgcgtgc cagaagcgct cgagcgaggc
     1021 tcgcgccccg tccggcaagc tgttcgcgta gtgcgcgagc gccgcgtcga gtttcggcag
     1081 atgggcgtcg tcgccttgcg ggtagatatg ccagacgaac ggcttttgcg cccattgcgc
     1141 ccgcacgaac gagtcctcgc cgcgcacgaa attcaggtcg gcggcccaga gcaaggcgtc
     1201 gaaaagcggc tgttcgacaa aacccagcgc ataggccgat agcgatccgc tttttgcctt
     1261 cgcccccgcc cggaagtccg gcaggtcgaa aaaccgggca atcgcaccgg aaatgcgtcc
     1321 ttccggcacg agcaacgcaa tgggcgtggc gcaatcgcgc cattgttcaa gcagcgaatc
     1381 gatggcggga ttctcgtacg cgaacatcga aatgacggtc gtaccggacg gcggcggcgc
     1441 gatgccgaca cgcgcgtgcc accaggcatc gcgatcaaac gcgtcacgcc cggcatcaag
     1501 caaacgctct ttcagcacac caccggtgcc gggacccagg ccgggaaaaa agaacgtttt
     1561 gtcgagcgcg tagcgcggat gcggcgacgg cctcagatga aaatccgcga cccagtcctc
     1621 cgcgctcaga tattcgagat tcacccacac gggcttcttc tcgcggcgcg ccatagcgct
     1681 cacgtacacc gccggcaatt cgcagccgaa cgcttcgatc acgacatcgg cgatctgcag
     1741 cgtgtcgccg gcatgcgacg gcgcatgcca gtgctcgatc atcacgcccg ccgcgctctg
     1801 gcggtcgagc gcgggatcaa cggcggggca gagcgcatga aaggccttga gatcatcgac
     1861 aaacagccgc acctgccagt cgtgttcgct ggccagttgg cgcgcgagcc gccagcagac
     1921 gccgatatcg ccgaaattgt cgatgaccgc gcagaaaata tcgcacgcga tgttgatggc
     1981 tggagcgccg gtgtcgacgc tggcgtggcc ggaagcagcc ggcaattgtt ccgatgaaga
     2041 cgacatgggc aatagggcgg cgatgaaccg ctagtgaatg gtgaagaatg gcggacagcg
     2101 gtttgaacta aactggcgtt ttcaaggata acgattcgcg cggcgcgctg cgttacggtt
     2161 gcgttatggt tgcgttacgg tcgagtcagg ggctggcctg ggcatgcgcc gtgtttcgtc
     2221 catactcagt ccatgctcaa tccatactcc gctcgaaacc aataaaatta ttttgctgaa
     2281 ctcaagctgc gcgcttctcg ccaacatctc tgcatgacag actccgacgt tttcgaccct
     2341 aaaaaaagcc tggctcaatt gccgcacttg ccgggcgtgt accgctatta cgacgagcat
     2401 ggcaacgtgc tgtacgtcgg gaaggcgcgc gacctgagaa aacgcgtgtc gagctatttt
     2461 acgaagacgc tgctgtctcc gcgcatcgcg atgatggtca cgcgcatccg caaaatcgaa
     2521 acaacggtca cacggtccga agctgaagcg ctgctgctcg agaataatct gatcaaggcg
     2581 ctcgcgccgc gctacaacat cctgtttcgc gacgacaagt cgtatccgta tctgaagctc
     2641 accggccaca agtttccgcg catggcgtat taccgtggcg gcgtcgacaa gaagaaccaa
     2701 tacttcggcc cgttcccaag cgcgtgggcc gtgcgcgaaa gtatccagat tctgcagcgc
     2761 gttttccagt tgcgcacctg cgaggattcc gtcttcacca atcgcacgcg gccttgcctg
     2821 ctgcatcaga tcgggcgctg taccggtcca tgcgtcgggc tgatcaacga agaggattac
     2881 gcgcgcgatg tgtcgaacgc atcgcggttc ctgctcggac gccagggcga agtgatgaag
     2941 gaactcgagc agaagatgca tgcgttcgcc gccgatctca aattcgaaca ggccgcggcc
     3001 gtccgtaacc agatgagctc gctgtccacg gtgctgcatc agcaggctat cgaggtaggc
     3061 ggcgataacg acgtcgatat cctcgcggtc gtcgcgctgg gcggcaaggt gtgcgtgaat
     3121 ctcgcgatgg tgcgcggtgg ccggcatctc ggcgataaag cctatttccc gacgcacgta
     3181 gaggcggcgt tggccataga agacgcgatg ccggaagagc ttgccggcga tgacttcgtg
     3241 gacggcgtgg cgaatatcgt cacgaatatc gtcattgagc aagtcgatgc ggatgtcgat
     3301 gcgcttgtcg atgaactcgc gctgccggcc ggcaccctcg cggcgcgcga gcaggacttc
     3361 gaggcccacg ctaccaccgg cgaaatggag gacgagatcg ccgaaacagg ggcggcgccg
     3421 ttgaatgaag cgacggaatt ggaacgtcag ggcgcgctcg aagctgaagt gctggaggcg
     3481 tttatcgccc agcattacat cggcaatcgc gtgccgccgg tgctggtggt gagccacgcg
     3541 ccaacgaacc gcgagctcgt cgacctcctg atcgaacagg ccgggcacaa ggtcgtggtg
     3601 ctgcgtcagc cgcagggaca aaagcgcgcg tggctgtcga tggctgagtc gaatgcaaag
     3661 ctcgcgctcg cgcgcttgct gtccgaacag ggatcgcagc aggcccgcac gcgttctctc
     3721 gcggacacgc tgagcttaga aatggacgat ctcgcgcagt tgcgcatcga atgtttcgac
     3781 atcagccaca cgatgggcga ggcgactcag gcatcgtgcg tggtgtatca ccatcacaag
     3841 atgcagtcgg ccgaatatcg gcgctacaac atcaccggca tcacgccggg tgacgattac
     3901 gccgccatgc gccaggtcct cacgcgccgc tacgagaaga tgctggcgca ggccgcggcg
     3961 cgcgcttctg aagctaatga tgaaccgcag caggaagcgg cgggattgga ggccgaagct
     4021 ccacttgagc cgaccagacc cgctgaagcc gatgtcgctg aacctgtgcc atcgggtggc
     4081 tgcttgccga atattgtgtt gatcgatggt ggcaagggac aggtcgagat tgcgcggcaa
     4141 gtgttcgccg aactgggcct cgatcgcagc atgctggtgg gtgtggcgaa gggggaaggg
     4201 cgtaaggtcg gtctcgaaac cctgattttc gccgatggcc gcgcagcgct cgaactcggc
     4261 aaggagagcg cggcgctgat gcttgtcgcg cagattcgcg acgaagcg
//
LOCUS       DATASH010000204         5699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_817372,
            whole genome shotgun sequence.
ACCESSION   DATASH010000204 DATASH010000000
VERSION     DATASH010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5699
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817372"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..1246
                     /locus_tag="VHV99_05680"
     CDS             68..1246
                     /locus_tag="VHV99_05680"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX3634969.1"
                     /translation="MTDAVIVSTARTGLAKSWRGGFNMTHGATLGGHVTQAAVERAKL
                     DPARIEDVIMGCANPEGATGMNIARQIALRAGLPVSVPGMTVNRFCSSGLQTIALAAQ
                     RVIAGEGDVFVAGGVESISCVQNEMNRHMVTEGWLSQNKPEIYGSMLQTAENVAKRYA
                     ISKERQDEYGVRSQQRAAAALEAGKFKDEIVPLTVLAGVADKASGRLFTKEVTVSADE
                     GIRADTTLEGVSKIRTAMPGGVITAGNASQFSDGASACVVMNAKVAEREGLQPLGIFR
                     GFAVAGCEPDEMGIGPVFAVPKLLRQAGLKVEDIDLWELNEAFAVQVLYCADKLGIPH
                     DRLNVNGGAIAVGHPYGVSGARLTGHALIEGKRRGARLVVVTMCIGGGQGAAGLFEVV
                     "
     gene            complement(1254..1772)
                     /locus_tag="VHV99_05685"
     CDS             complement(1254..1772)
                     /locus_tag="VHV99_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HEX3634970.1"
                     /translation="MSFLRVLLCRRHARLAAILAVVALTLNGCAGLFGGDPLRVSVAG
                     IEPVEGQGLEMRFNIKLRVQNPNDSAVQFDGVSLDLELNGKPFASGVSDQSGTVPRFG
                     ETVVNVPLTVPAFTAVRQAFAFAGAVQAGAAQSGQIPYVLRGKLAGGLAGTTRFVNQG
                     TLSLPSLGPALP"
     gene            2011..2499
                     /locus_tag="VHV99_05690"
     CDS             2011..2499
                     /locus_tag="VHV99_05690"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX3634971.1"
                     /translation="MTTDSPSSKAGSASLPESPFVDRLGAQLVSAADGVSEVVLPLQP
                     DHMNTWDVAHGGVTMTLADVALAMAARSLAGDGVGVVTVEMKVNFMQPGRGELRATGR
                     VLHRSTTMAYCEGEIRDSEGHFVAKALGTFKYMRRLAVGRDVTQQRLRTDPSAKPGPS
                     DG"
     gene            2611..3609
                     /locus_tag="VHV99_05695"
     CDS             2611..3609
                     /locus_tag="VHV99_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX3634972.1"
                     /translation="MPQINRQFVLASRPQGAVTPDNFRLVETPLAPLADGEFRVRNHF
                     LSLDPYMRGRMNDSKSYAAPQPLNEVMIGGTVGEVVESKNPKFALGDKLVAMFGWQEY
                     GTSNGAGVQKVDDTHVPLSAYLGPVGMPGVTAWYGLNRIIAPKAGETVVVSAASGAVG
                     SVVGQLAKLAGARAVGIAGGADKCRYVVETLGFDACVDYKAGNLYQDLKAVTPNGVDG
                     YFENVGGEVLDVTLARMNAFGRIALCGFISGYDGQPVPLKHPSLMLTQRLLVQGFIVS
                     EHMDVWPEALKQLGTLVAQKKLQYRETIAQGLDAAPEAFIGLLKGKNFGKQLVKLI"
     gene            3751..4650
                     /locus_tag="VHV99_05700"
     CDS             3751..4650
                     /locus_tag="VHV99_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3634973.1"
                     /translation="MFEFAGKVAVITGAASGFGRAFAEKGASLRMKLVLADVNPDALA
                     QTVDALRAGGAEVIGVPTDVADAAQVEALAQAALEAFGKVHLLFNNAGVGSGGFLWES
                     SANDWAWVFNVNVMGVAHGVRVFTPIMLRQNEPAHIVNTASVAGLLSPPAMGIYNASK
                     HAVVSLTETLYHDLQLAQAANPAPLAGSGEVGCSLLCPAFVPTGIADAERARPAELRN
                     DSGPTRSQIAAGKQLQRAVQAGKLTAADVADITFEAIAERRFYIVTHPGIMATVKLRH
                     EDIEQLRNPTDPMSLKPEVKRAS"
     gene            4713..5672
                     /locus_tag="VHV99_05705"
     CDS             4713..5672
                     /locus_tag="VHV99_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3634974.1"
                     /translation="MPLNPKIEQVLDMIARAKRPQLHDLTPQQARASYEKSAPILEIA
                     SAPMFSVEDLQVPTRDGATIRARLYQPVEPSWAEPAPALVYYHGGGFTVGSVDTHDAL
                     CRMFARDGRCAVLSVDYRLAPEYKFPTAVDDAFDALSWLHAHAAEYGVDAARLAVGGD
                     SAGGTLATVCAVLAREAGIELVLQLLIYPGATGYQQTDSHSRLANGFLLSGDTIQWFF
                     NQYVRDKSDRDDWRFAPLDGQHNAPDFSGLAAAWIATAEYDPLSDEGDAYAEKLRAAG
                     NQVALKRYPGMIHEFFKMGGFVPDVAEAHADAAAALRTAFGVE"
BASE COUNT         1044 a         1735 c         1951 g          969 t
ORIGIN      
        1 actgaggtgg cgcggtgcgg cgaatcggcc gcgcgccatc atcgacatct ggaagtagga
       61 gacttgcatg actgacgccg taatcgtatc gaccgcccgt acgggtctcg ccaaatcatg
      121 gcgcggcggt tttaacatga cgcacggcgc gacgcttggc gggcatgtga cgcaggccgc
      181 tgtcgaacgc gcgaagctgg acccggcgcg cattgaagac gtgatcatgg gttgcgcgaa
      241 tccggaaggc gcgacgggca tgaacatcgc gcgccagatc gcactgcgcg ccggtttgcc
      301 ggttagcgtg ccgggcatga cggtgaaccg tttctgctcg tccggtttgc agacgatcgc
      361 actggctgcg caacgcgtga tcgccggtga gggcgatgtg ttcgtcgcgg gtggcgtgga
      421 atcgatctcg tgcgtgcaga acgagatgaa ccgccacatg gtgaccgaag gctggctcag
      481 ccagaacaag ccggaaattt acgggtcgat gctgcagacc gccgagaacg tcgcgaagcg
      541 ctacgcgatc tcgaaggaac gtcaggacga atacggtgtg cgctcgcaac agcgcgccgc
      601 ggccgcgctc gaagccggca agttcaagga cgagatcgtg ccgttgacgg tgctggccgg
      661 tgtcgccgac aaagcgagcg ggcgtctgtt cacgaaggaa gtcaccgtca gcgccgacga
      721 aggcattcgt gccgacacga cgctcgaagg cgtgtcgaag attcgtaccg cgatgccggg
      781 cggcgtgatc acggcaggca acgcgagcca gttctcggac ggcgcgtcgg cgtgcgtcgt
      841 gatgaacgcg aaagtcgcgg agcgcgaagg tctgcaaccg ctcggcattt tccgcggctt
      901 tgcggtggcc ggctgcgagc cggacgagat gggcatcggc ccggtgttcg ccgtgccgaa
      961 gctgctcagg caggccggct tgaaggtcga agatatcgac ctgtgggaac tgaacgaagc
     1021 gttcgcggtg caggtgctgt attgcgccga caaactcggc attccgcacg accgcctgaa
     1081 tgtcaacggc ggcgcgatag cggtgggcca tccgtatggt gtgtcgggcg cgcgtttgac
     1141 cggccatgcg ctaatcgaag gcaagcggcg cggcgcgagg ctggtcgtcg tgacgatgtg
     1201 tatcggcggc ggccagggcg cggcgggttt gttcgaggtg gtgtgatcag gcgtcagggc
     1261 aaagccgggc cgagcgacgg caggctcaac gtaccttgat tgacgaagcg tgtcgtgccc
     1321 gccaggccgc cggcgagctt gccgcgcagg acgtacggaa tctgacccga ctgcgcggcg
     1381 cccgcttgca cggcgcccgc gaaggcgaac gcctggcgaa ccgcggtgaa ggcgggcacc
     1441 gtcaacggta cgttgacgac cgtttcgcca aagcgcggca cggtgccgct ctgatcgctg
     1501 acgccgctgg cgaacggttt gccgttcagt tcgagatcga gcgacacgcc gtcgaactgg
     1561 acagcggaat cattgggatt ctgcacgcgc agtttgatat tgaagcgcat ctccaggcct
     1621 tggccttcga ccggttcgat accggccacg ctcacgcgca aagggtcgcc gccgaacaga
     1681 ccggcgcagc cgttgagcgt cagcgcgacg acggcgagga tggcggccag acgggcgtga
     1741 cggcggcaca gcagaacgcg aaggaaggac atgaccggca cctcgaagga agcgggttgg
     1801 gtcatgcatt gtagcgagct gtttctgggc cggtggggac gttcgagcga cgtagcccca
     1861 tgcggcgtga tctgatgagt ggccggtgag cgcgctgccg ttacgcattc ggccttatcc
     1921 gttaggcgtt cgcgcgtggc ccccacgcat gcgcgaacgc cttagcggtt atcatttgag
     1981 gtcccaggcc aacgaattga atcgaatcag atgaccaccg attcaccctc atccaaagca
     2041 ggcagcgcgt cgctgcccga aagtccgttc gtcgaccggc tcggcgcgca actcgtatcg
     2101 gccgcagatg gcgtcagcga agtcgtgctg ccgttacagc ccgatcacat gaacacgtgg
     2161 gatgtcgcgc atggcggcgt gaccatgacg ctcgccgacg tcgcgctcgc gatggccgcg
     2221 cgcagtctgg ccggcgacgg cgtcggcgtg gtcaccgtcg agatgaaggt caacttcatg
     2281 cagccgggcc gcggcgaatt gcgcgccacc ggccgtgtgc tgcaccgctc gaccaccatg
     2341 gcctattgcg agggcgagat tcgcgacagc gaaggccact tcgtcgccaa agcgctcggc
     2401 acgttcaaat acatgcggcg tctcgccgtg ggccgcgacg tgacgcaaca gcgtctgcgc
     2461 acggaccctt cggcgaaacc cggtccaagc gacggctaat caccggatgg accgcgcgtg
     2521 ctacgagtcg cgcgctggat ccggttgttg ctgacgccgt tcgttcgaat tactcaccga
     2581 tcacaccggt aacccatgga gacgatcgtc atgccccaga tcaaccgtca atttgtgctg
     2641 gcttcgcgcc cacaaggcgc tgttacgccc gataacttca ggctcgtcga aacgccgctc
     2701 gcaccgctcg ccgatggtga attccgcgtg cgcaaccatt ttctctcgct cgatccgtat
     2761 atgcgcggcc ggatgaacga cagcaagtcg tacgcggcgc cgcagccgct gaacgaagtg
     2821 atgatcggcg gcacagtggg cgaagtggtg gagtcgaaga atccgaagtt cgcgctcggc
     2881 gataagcttg tcgcgatgtt cggctggcag gaatatggca cctcgaacgg tgcgggtgtg
     2941 cagaaagtgg atgacacgca cgtgccgctg tcggcgtatc tcgggccggt tggcatgccg
     3001 ggcgtcaccg cctggtacgg cttgaacagg atcatcgcgc cgaaagcggg tgaaacggtg
     3061 gtggtgagcg cggcgagcgg cgcggtgggc agcgtggtcg gccaactggc gaagctggcc
     3121 ggtgcgcgcg cggtcggcat cgcgggcggc gcggacaagt gccgctatgt ggtggagacg
     3181 ctcggcttcg atgcctgcgt cgactacaag gccggcaatc tgtatcagga tctcaaagct
     3241 gtgacgccga acggtgtcga tggttacttc gagaacgtcg gtggcgaagt gctcgacgtg
     3301 acgctcgcgc gcatgaacgc gttcggccgt atcgcactgt gcggctttat ctcgggctac
     3361 gatggccagc cggtgccgct caagcatccg tcgttgatgc tcacgcagcg gctgctcgtg
     3421 cagggcttca tcgtcagcga acatatggat gtgtggcccg aagcgctcaa gcaactcggc
     3481 acgctggtcg cgcaaaagaa gctgcaatac cgcgagacca tcgcgcaagg gctcgatgcg
     3541 gcgcctgagg cgttcattgg tctgctgaag ggcaagaact tcggcaagca gctcgtcaag
     3601 ctgatctgat cgcggcggct gtcgcggcgg ctgtcaagcg gcctacgcga gaaacgttcg
     3661 tgaaggttcg ccggtgaacg taaaccggtg gacctggccc gatgcactta acctgttccc
     3721 ccgaagcgca aaacccagga gcaaaaaccg atgttcgaat tcgcaggcaa ggtggcggtg
     3781 atcaccggtg cggcaagcgg cttcggccgc gcgttcgcgg aaaagggcgc gtcgctccgc
     3841 atgaagctcg tgctcgccga cgtcaatcct gacgcgcttg cgcaaaccgt cgacgccttg
     3901 cgcgccggcg gcgccgaggt gatcggcgtg ccgaccgacg tcgccgacgc cgcccaggtc
     3961 gaagcgctcg cgcaagcggc gctcgaggcc ttcggcaagg ttcatcttct gttcaacaac
     4021 gcgggcgtgg gttcgggcgg cttcctgtgg gaaagctcgg cgaacgactg ggcatgggtg
     4081 ttcaacgtca acgtgatggg tgtcgcgcac ggcgtgcgcg tcttcacgcc gatcatgctg
     4141 cggcagaacg aacccgcgca tatcgtcaac acggcgtcgg tggcgggctt gctgtcgccg
     4201 cccgcaatgg gcatctacaa cgcctcgaag cacgccgtcg tgtcgctcac ggagacgctc
     4261 tatcacgacc tgcaactggc ccaggcggca aacccggccc cgctggcggg tagcggcgag
     4321 gtcggctgct cattgttgtg cccggccttc gtgccgaccg gcatcgccga cgcggaacgc
     4381 gcgcggcccg cggaactgcg caacgacagc ggcccgaccc gctcgcagat cgctgccggc
     4441 aagcaactgc aacgcgcggt gcaagcgggc aagctgactg ccgccgacgt cgccgacatc
     4501 acgttcgaag cgatcgccga gcggcgtttc tatatcgtca cgcatccggg catcatggcg
     4561 acggtgaaac tgcgtcacga ggatatcgag cagttacgca atccgaccga tcccatgtcg
     4621 ttgaagcccg aagtgaagcg tgcgagctaa gcgcttacgg catcagcatc acatccacca
     4681 acattcggcg ccacgcgcca tcagacgcaa aaatgccgct gaatcccaag atcgagcagg
     4741 tgctcgacat gatcgcgcgc gcgaagcgcc cgcagcttca cgacctgacg ccgcagcagg
     4801 cgcgcgcgtc ttacgagaaa agcgcgccga ttctggagat cgcgagcgcg cccatgttct
     4861 cggtggaaga tctgcaagtg ccgacgcgcg acggcgcgac gattcgcgcg cgtctttatc
     4921 agccggtcga gccgagctgg gccgagcccg cgccggcgct ggtgtattac cacggcggcg
     4981 gcttcacggt cggcagtgtc gatacgcacg acgcgttgtg ccgcatgttc gcgcgcgatg
     5041 gccggtgtgc ggtgttgtcg gtcgattacc ggctcgcgcc cgagtacaaa tttcccaccg
     5101 ccgttgacga tgcattcgat gcgttgagct ggctgcatgc gcacgccgcg gaatacggag
     5161 tggatgcagc gcggctggcg gtgggcggcg atagcgcggg cggcacttta gccacggtgt
     5221 gcgcggtact ggcacgcgag gcgggcatcg aactcgtgct gcaattgctg atctatccgg
     5281 gcgccacggg ctatcagcaa accgattcgc actcgcggct tgccaacggc ttcctgctgt
     5341 cgggcgacac gattcaatgg tttttcaatc aatacgtgcg cgataagagc gaccgcgacg
     5401 actggcggtt cgcgccgctc gacggccagc acaatgcgcc ggatttcagc ggcctcgcgg
     5461 cggcatggat tgcgaccgcc gaatacgacc cgctaagcga tgagggcgac gcgtacgcgg
     5521 agaaactgcg cgcagcaggc aatcaggtcg cgctgaagcg ctacccgggc atgattcacg
     5581 agttcttcaa gatgggcggc ttcgtgcctg atgttgccga ggcgcatgcg gatgccgctg
     5641 ctgcattgcg cacggcgttc ggggtcgagt agtcggcgcc ctgagtcgaa cgatcgtca
//
LOCUS       DATASH010000205         5224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_817572,
            whole genome shotgun sequence.
ACCESSION   DATASH010000205 DATASH010000000
VERSION     DATASH010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5224
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817572"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1341)
                     /locus_tag="VHV99_05710"
     CDS             complement(1..1341)
                     /locus_tag="VHV99_05710"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HEX3634975.1"
                     /translation="MYFLTNRKMPRRTFLRGVGATIALPFLESMLPARGARAVEAKVL
                     PTRFVGAFVPHGAAPGYWVPESTQAGFKFPFIYEPLVPFRKQTIITSGMWAQSSENPP
                     GVTGADHFVAAAYLTAAKPKKTTGADIHVATSIDQIIAQKIGTETLLPSLQIGLEDAS
                     ANSSTCGEGYSCVYTNTISWQAPDRPLPMEINPQTIFERMFGDGSSPELRKARREQQA
                     SILDSVNGSLNELLPEVSHSDRVRLEQFTEDVREIERRMGIAAKASSEVPNVAVPYGV
                     PESFHEHIQIQSDLLSLAFQGDITRVATMLYARDLTGRVYPESGTNISFHGGSHHAED
                     PSRVADYAKLNRYHVQMLAYLVKKLNDIKEGDGTLLDRSLILYGSNMGDSNQHLHYDV
                     PAVLIGGASGKMKGDKHIAVASRTVPTGNLLLSILDKFDIKADRFGDSTGRLAI"
     gene            complement(1350..3887)
                     /locus_tag="VHV99_05715"
     CDS             complement(1350..3887)
                     /locus_tag="VHV99_05715"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HEX3634976.1"
                     /translation="MTKWSLIAAVGGTALLMPLIASAGNDEKVGIAQRQLLDKYCIVC
                     HNYTDYAGGLEFETYDPTAPQDDAKVTEKMLKKLRVGMMPPAGKDRPDPAAVHNLINS
                     LETKIDAHEKASLSVPKLHRLNRGEYANAVRDLLALDIDTSKFLPPDDSSRGFDNQAG
                     ALTLSSALLDAYLSAAARVSRVAIGEAAAPAQVTYRIPQGTTQNYHVEGLPLGTRGGI
                     EIDHTFPSDGNYTFKVIAVVLGNMGSDRPFGEVRGEQLLVYVDDKRVAKIDWDKALGV
                     GRRFAATADDEGGAAGQLKPIDVKLPVTAGPHKIGVTFLATNFAPGLDMNHAFDRSTI
                     ETGGLPGFTFYPHVGSVRIDGPTDAKAAADSPSRERIFVCHPSNASDEETCARQITTT
                     LAHRAYRGYETPQNVDTLMGFYTSGRRSGSFDDGIETMVQRVLVDPKFLFRVEHTPTG
                     VAAGAPYHVNDLELASRLSFFLWSSVPDDTLLQLAESKQLTKRDVLQKQVHRMLGDPR
                     AEALTRNFASEWLGLRSLQGHDPVTDQFPDFDDNLRDAFREEAQLLFTSLLQEDRSVL
                     DLLTANYTFVNERLAKHYGIPGIRGSEFRRVKLEGDLAARQGILGTGAMLTVSSQPGR
                     TSPVIRGQWVLRTLLGTPAPDPPANVPALDAKTTDQAGNGKVPTMREALAAHRDKPQC
                     AGCHKMMDPIGFALEPFDAVGHTRTVDQSGQLINAKDVMYDGTAVDGVAGVRTWVLKY
                     KDQYVQNVVANLLTYALGRGVEYDDMPTVRNIAHTSARDGYKLKTIIEAITASDVFQM
                     NVAPSGDGDKSKGTDVLPCRAPGASRDSLRCAPVTTTSTAVHHPPGG"
     gene            complement(3899..>5224)
                     /locus_tag="VHV99_05720"
     CDS             complement(3899..>5224)
                     /locus_tag="VHV99_05720"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX3634977.1"
                     /translation="RVPVMAALLEKGAKVDEKEKLRGTTALMWAAANSNTDAVRFLVS
                     KGADVRARSGTTAPGRPPYLAPPGRNRIEDFINGKGITFEGVKPSSDPKAQAEEEKIL
                     AKEREYAKRSLAAFPLDKSTKPAPKKWGGLTPLQFAVREGSMETVKVLVDSGADVNQR
                     SEFGWTALLVATQNRYYKIGVFLLDHGADPNIANEGGWTPLYIATDNRNIEGGDYPTR
                     KPDMDHLDYIKRLFVSNANPNLRMRSSTETRTIFTHQWLLEEGATPFLRAAQSGDLVL
                     LKLLLEHGADPKVNTDDGVTPLMVASGIGWVEGVTYEWSPQQTLETVKMLLDLGADVN
                     AQDVIDGRTALMGAAHKGRNDVIDLLVQHGADLSLRDIGSRDTINSLSGLRFQAIDYA
                     DGLVRIGVQSAIAHPETSALLRKMMRERNLPVPQDGRTLASICITDLCK"
BASE COUNT          906 a         1619 c         1654 g         1045 t
ORIGIN      
        1 ttatatcgcc agccgtccgg tgctgtcacc gaagcggtcg gccttgatgt caaacttgtc
       61 caggatgctc agcaacagat tgcccgtcgg caccgtccgg ctcgcgacgg cgatgtgctt
      121 gtcgcccttc atcttgccgg aagcgccgcc gatcaacacg gcaggcacgt cgtagtgcag
      181 gtgctggttg gaatcgccca tgttcgaccc gtagaggatc agagagcgat ccagcagtgt
      241 gccgtcgcct tccttgatgt cgtttagctt cttcacgagg tacgcgagca tctgcacgtg
      301 gtagcggttg agtttcgcgt aatccgcgac tcgactcgga tcctcggcgt gatgagagcc
      361 accgtggaag ctgatgttgg taccgctttc cggatatacg cggccggtca ggtcgcgagc
      421 gtagagcatt gtcgccacgc gcgtgatgtc gccctgaaag gcaagcgaca acagatctga
      481 ctgaatctgg atgtgctcgt ggaacgactc cggcacgccg taggggacag ccacgttcgg
      541 cacttcggaa gatgctttgg ctgcaatgcc catgcgacgc tcgatctcgc gcacgtcctc
      601 tgtgaactgc tccagccgta cgcggtcgct gtgcgaaact tccggcagca gctcattcag
      661 gctcccgttg acggagtcga ggatgctggc ctgttgctcg cggcgcgcct tgcgcagctc
      721 gggcgagctg ccgtcgccaa acatgcgctc gaaaatcgtc tgcggattga tctccatggg
      781 cagcggccgg tcgggagcct gccaggaaat cgtgttggtg tagacgcagc tgtagccttc
      841 gccacaggtc gaggagtttg cgctcgcatc ttccaaaccg atctgcagag agggcaagag
      901 ggtctcagtg ccgatcttct gcgcgatgat ctgatcgatg ctggtggcga catggatatc
      961 cgcacccgtc gtcttcttcg gctttgcagc tgtgaggtac gcggcggcga cgaagtgatc
     1021 ggcgccggtc acacccggcg gattctccga tgactgggcc cacatgccgc tcgtgatgat
     1081 ggtctgcttg cggaacggca cgagcggctc gtatatgaac ggaaacttga agccggcctg
     1141 cgtgctctcc ggcacccagt acccaggcgc cgcgccgtgc ggcacgaacg cgccgacgaa
     1201 acgtgtcggc aacaccttcg cttccacggc tcgtgctcca cgggccggta gcattgattc
     1261 gaggaaaggc agcgctatcg tcgcgcccac accgcgcagg aacgttcggc gcggcatctt
     1321 ccgattcgtc aggaaataca tggcctcagt catcctcctg gcgggtggtg aaccgccgtt
     1381 gatgtcgtgg tgaccggagc gcagcggagg gaatcccgag aagcgccagg ggcgcgacac
     1441 gggagcacat ccgtaccttt gctcttatcc ccgtcaccgc tcggagcaac gttcatctgg
     1501 aacacgtcac tcgccgtaat cgcttcgata atggtcttca acttgtagcc atcgcgtgcc
     1561 gaggtgtgag cgatgttgcg cacggtcggc atgtcgtcgt actccacacc gcgcccaagc
     1621 gcgtacgtga ggaggttcgc cacaacgttc tgcacgtact gatccttgta cttcaacacc
     1681 caggtgcgga cgcccgcgac gccgtcaaca gcggtgccgt cgtacatcac gtctttcgcg
     1741 ttgatcagct ggccgctctg gtcgaccgtg cgtgtatggc ccacagcgtc gaacggctcg
     1801 agcgcgaacc caatgggatc catcatcttg tggcagcccg cacattgcgg cttgtcgcgg
     1861 tgggcggcga gcgcttcgcg catcgtaggc accttgccgt tgccggcctg gtccgtggtc
     1921 ttcgcatcga gcgcgggcac gttggctgga ggatccggcg ccggcgtgcc gaggagtgtg
     1981 cgcaacaccc attggccgcg gatcacaggc gaagtacggc ccggctgcga cgacaccgtc
     2041 aacatcgcgc ccgtgccgag aatgccctgg cgcgcagcca aatcgccttc gagcttcacg
     2101 cggcggaact cgctgccgcg gatgccgggg atgccgtagt gcttcgcgag acgctcattc
     2161 acgaaggtgt agttcgcggt gagcaggtct aagacgctgc ggtcttcctg cagcaggctg
     2221 gtgaagagca gctgtgcttc ttcccggaag gcgtcgcgca agttgtcgtc gaagtccggg
     2281 aactgatcgg tgaccggatc atgcccctgc aggctgcgca gccccagcca ctccgaggcg
     2341 aagtttctcg taagtgcttc cgcgcgcggg tcgcccagca tgcgatgcac ctgcttctga
     2401 agaacgtcac gcttcgtgag ttgcttcgac tccgcgagct gcagcagcgt gtcgtccggg
     2461 acgctgctcc agaggaagaa cgaaagccgc gaagcgagct cgagatcgtt tacatgatac
     2521 ggagcgcccg ccgcaacgcc agtcggcgta tgttccacgc gaaagaggaa cttcggatcg
     2581 acgagcaccc gctgcaccat cgtttcgatg ccgtcgtcga aggaaccact gcgccgaccg
     2641 cttgtgtaga agcccatcag cgtgtctaca ttctgcggcg tctcgtaacc gcgatacgcg
     2701 cgatgcgcca gcgtcgtcgt aatctgacgc gcacaggtct cttcatcgct cgcattcgac
     2761 gggtgacaca cgaagatgcg ttcgcggctc ggtgaatccg cggcggcctt ggcgtccgtc
     2821 ggcccatcga tgcggacgct gccgacgtgt gggtagaagg tgaagccggg caacccaccg
     2881 gtttcgatcg tgctgcgatc gaaggcgtgg ttcatgtcga ggcccggcgc gaagttggtg
     2941 gccaggaacg tcacgccgat cttgtggggg ccggccgtga cgggcagctt gacgtcgatg
     3001 ggcttcagct ggccggcggc accgccttcg tcatccgctg tagctgcgaa acggcgaccc
     3061 acgccgagag ccttatccca atcgatcttg gcaacgcgct tgtcatccac atacacgagc
     3121 agctgttcgc ccctgacctc gccgaacggg cggtcgctgc ccatgttgcc gaggaccacg
     3181 gcgatcactt tgaaggtgta gttgccgtcc gacgggaacg tgtgatcgat ctcgatgcca
     3241 ccgcgcgtgc cgaggggcag gccttcaacg tgatagttct gcgtcgtacc ttggggaatg
     3301 cggtaggtca cctgcgcggg cgccgccgct tcgccaatcg cgacacggct cacgcgagcg
     3361 gcggccgaga gatacgcgtc gagcagagca gaggacagcg tcagcgcacc cgcctggttg
     3421 tcaaacccac ggctcgaatc gtcgggcggc aggaatttcg acgtatcgat atccaacgcc
     3481 agcagatcgc gcaccgcgtt cgcgtattcg ccgcgattca gacgatggag cttcggaacg
     3541 gaaagactcg ccttctcgtg cgcgtcgatt ttcgtctcga gggagttgat cagattgtgg
     3601 accgcggccg ggtcgggccg atccttgcct gcgggcggca tcatgccgac gcgcagcttc
     3661 ttcagcattt tctcggtgac cttcgcatcg tcctgcggag ccgtcggatc gtaggtctcg
     3721 aattcgaggc cacccgcgta atccgtgtag ttgtggcaga cgatgcagta cttatcgagc
     3781 aattggcgct gggctatccc aaccttttcg tcgttacccg ccgaagcgat gagcggcatc
     3841 aggagcgcag tgccgccaac ggcggcaatc agggaccact tagtcattct tgttgaattc
     3901 atttgcacag gtcggtgatg cagatcgaag ccagcgtgcg cccgtcctgc ggcaccggca
     3961 gattccgctc cctcatcatc ttgcgcagga gcgcagaggt ttccggatgc gcgatagccg
     4021 attgcacgcc gatgcgcacg agcccgtccg cgtaatcgat cgcttggaag cgcaggcccg
     4081 aaagcgagtt gatggtgtcg cgactgccga tgtcacgcag cgacaggtcg gccccgtgct
     4141 gtacgagcag gtcgatcaca tcgttgcggc ccttgtgtgc agctcccatc agcgccgtgc
     4201 gaccgtcgat cacgtcctgc gcattcacgt cggcgcccag atccagcagc atcttcacag
     4261 tctcgagcgt ctgctggggt gaccactcat acgtgacgcc ctcgacccag ccgatgccgc
     4321 tcgccaccat cagcggcgtc acgccgtcat ccgtgttgac cttcggatcg gcgccgtgct
     4381 cgagcagcag cttcaacagc acgaggtcgc ccgactgcgc ggcacgcagg aacggcgtcg
     4441 ccccctcttc caggagccac tggtgcgtga agatggtgcg tgtctcggtg ctcgagcgca
     4501 tgcgcaggtt cgggttcgcg ttggacacaa acagccgctt gatgtagtcg agatggtcca
     4561 tgtccggctt gcgcgtggga tagtcgccgc cttcgatgtt cctgttgtct gttgcgatgt
     4621 aaagcggggt ccagccgcct tcattggcga tgttcggatc ggcgccgtga tcgagcagga
     4681 agacgccaat cttgtagtag cggttctgag tggcaaccag cagcgctgtc cagccgaact
     4741 cactgcgctg gttcacgtcg gcgcccgagt cgaccagcac tttcaccgtc tccatgctgc
     4801 cttcacgaac agcgaactgc aggggtgtga ggccgcccca cttcttgggg gcgggtttgg
     4861 tggacttgtc cagcgggaaa gcggcaagag aacgcttggc gtattcgcgt tctttggcca
     4921 gtatcttttc ttcctcggcc tgcgctttgg ggtcactcga gggcttcacg ccttcgaagg
     4981 tgatcccttt gccgttgatg aaatcctcaa ttcggttgcg gcccggcggc gccagatagg
     5041 gtgggcgacc cggcgcagta gtgcccgagc gggccctgac atcggcaccc tttgaaacca
     5101 ggaaacgaac cgcgtcggtg ttggagttcg cggcggccca catcaaggcg gtcgtgccgc
     5161 ggagcttttc tttctcgtcg accttggccc ctttttccaa cagggcggcc atgaccggaa
     5221 cgcg
//
LOCUS       DATASH010000206         3625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4772522,
            whole genome shotgun sequence.
ACCESSION   DATASH010000206 DATASH010000000
VERSION     DATASH010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4772522"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VHV99_05725"
     CDS             complement(<1..463)
                     /locus_tag="VHV99_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEX3634978.1"
                     /translation="MSEAVSNQPKRSRRGLIIFAVIVLALIAVVAVHLLTRKKPSREA
                     APIVVTVASATLGSMPVTLSELGTVTPIATVTVLPQLSGYLTAVGYHEGQEVQKGQFL
                     AQIDPRQFEISKQQAEAQLAKDKASLAQAKSDLVRFTQLNEHKSIAEQTYVD"
     gene            complement(970..1791)
                     /locus_tag="VHV99_05730"
     CDS             complement(970..1791)
                     /locus_tag="VHV99_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX3634979.1"
                     /translation="MIVAVADTGNISRAAEVVHRSQSAVSMQIKTLETALGKPLFVRK
                     PRNVIPTQDGEVLLTYARRMLALRDEAWAAVVRPDVTGRVSIGVPDDYASSLLPPILK
                     KFSASYPKVEIQVVGLPSVALAPMVKDGSVDLVCATRVKGLSGEFIRLEPMEWAAAPS
                     ANEIWRERPLPIAVFLPGSVARENAIRSLEHAKIPYRTSYESPSLLGLLSMAEAGLAI
                     VPLARCAVPAHFTLLGRAHGLPEITALEMVLARSTASKRPPCDFLAEKILAELRR"
     gene            1918..2769
                     /locus_tag="VHV99_05735"
     CDS             1918..2769
                     /locus_tag="VHV99_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3634980.1"
                     /translation="MNARTDPSVLALQELSFATIASGICVPYVERGDGEPLIFVHGSL
                     CDYRYWNAQTAALSQHFRCISVSLSHYWPASEACIQGEFGWQTHVAELAEFIEAIDLG
                     PVHLVGHSRGGCVAFHVAREYPRLVKTLTLADPGGPLQIEGMPDASLPAATNVLRARV
                     AELIESQQIDAGLELFVDSVSVPGSWRKSPIAFRTMAIDNAGTLPKQLRDPLPAYSRE
                     TARDVKCRTLLIEGQRSPRMFRNNVEKLAEWIDYAERQTIAGASHGMNVSNAGAFNRL
                     VHAFASF"
     gene            complement(2866..3195)
                     /locus_tag="VHV99_05740"
     CDS             complement(2866..3195)
                     /locus_tag="VHV99_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3634981.1"
                     /translation="MSKATSTLIAALAALTLSGGAYAQAAAGSSGTGSAGSTAGPANQ
                     TNQTNGATAGYGAPGTTNSDSGMAARSPNSGLPSSTNSSSTSGTSAPKSNNTLATPSV
                     VSPAAGQ"
     gene            3430..>3625
                     /locus_tag="VHV99_05745"
     CDS             3430..>3625
                     /locus_tag="VHV99_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX3634982.1"
                     /translation="MRTVEYRFSTIWRIDAPVQEVWAAIQDSARWPEWWNNVERVDQL
                     EAGSDQGVGLVQRYTWKGWLP"
BASE COUNT          677 a         1075 c         1150 g          723 t
ORIGIN      
        1 ggtcgacgta ggtctgctcg gcgatcgact tgtgctcgtt cagttgcgta aagcgcacga
       61 ggtccgattt ggcctgcgcg agcgacgcct tgtctttcgc gagttgtgcc tcggcctgct
      121 gcttgctgat ttcgaactgg cgcggatcga tctgcgcgag gaactggcct ttctgcactt
      181 cctggccttc gtgataaccg acggctgtga ggtagccgct caactgcggc agcacggtga
      241 cggtggcgat cggcgtgacg gtgccgagtt cgctcaacgt gaccggcatc gagccgagtg
      301 tcgcgctcgc cacggtgacg acaatgggtg cggcttcgcg tgagggcttc ttgcgcgtca
      361 acagatggac agcgacaacc gcgatcagcg ccaacacgat aaccgcaaag ataatgagcc
      421 cgcgccggga acgctttggt tggttggaca cggcttcgct cataggtctc tccaaaaacg
      481 gatgctgccg ctgatgatcg ggcggggcgg gatgctctgt tgggttttgg ggaacatgcc
      541 acggttctcg tgcgttgtca caaatcaacg gccttttgaa ttgtgttagc taattgaaag
      601 taaaaaggat tgcgcaaacc gcataactcg cgaccggcgc aagcgcgtga aagcgcggcg
      661 acagggtccg tgtcgatgaa cgcgtttcgt aatgggcggt aacgtcttac gaagcgagcc
      721 cggcgaaata gtgcatgcgg cgcattgccg acagccgcac ggctaccgcg ggcgctttgg
      781 tgacagatcg taggttgctc atgttcggct gggtggaagc gcatagacag cgattggaca
      841 agacatggcg acaatgtacc cgggccgcgc aagacttgag ttaccagcct gaaacatctt
      901 ggggtgcgaa agccgttgcg acaaacgttc gaatgcgccg gacggcaact gggtaatcca
      961 catccaccgc taccgccgca actccgcgag aatcttctcc gccagaaaat cgcaaggcgg
     1021 ccgcttcgag gctgtgcttc gcgcaagcac catctcgagc gcggtaattt cgggcaagcc
     1081 gtgcgcgcgt ccaaggaggg tgaagtgcgc cggcaccgcg catcgtgcga gcgggacaat
     1141 cgccaggccg gcttcggcca ttgacagcag gcccagcaga ctcggacttt catacgaggt
     1201 ccggtagggg atcttcgcgt gctcgaggct gcgaatcgca ttttcccgcg cgacgctgcc
     1261 cggcaggaac acggcgatcg gcagcggccg ttcacgccat atttcgttgg cgctcggcgc
     1321 cgccgcccat tccatcggtt caagccggat gaactcgccc gacaagccct tgacccgcgt
     1381 cgcgcacacg aggtcgacgg aaccatcttt caccatgggc gcgagggcca cgctcggcaa
     1441 gcctacgacc tgaatctcca ccttcggata gctcgcggaa aacttcttga ggatcggcgg
     1501 cagcagcgac gacgcgtaat cgtcaggcac gccgatactc acgcgccccg tcacgtccgg
     1561 cctgaccacg gccgcccagg cctcgtcgcg caatgcgagc atgcggcgag cgtacgtcag
     1621 cagcacttcg ccgtcctgcg tggggatcac atttctcggc ttgcgaacga acagcggctt
     1681 gcccagcgcg gtctcgagcg tcttgatctg catgctcacc gccgattgcg aacgatgcac
     1741 gacttccgcc gcccggctga tgttgccggt gtcggcgacg gcgacgatca tggtcatgac
     1801 gtccaggtcg agagctttca attgggctgg tatcagaaaa atagatggat tcgatcaata
     1861 ttacgcgatt ttctaacgtg gcggcatcgg gcatagtgat cgccaaggag ccacacaatg
     1921 aacgcccgta ccgacccctc agtgcttgcc ctgcaggaat tgtcctttgc gaccatcgca
     1981 tcgggcatct gtgtgccgta tgtcgagcgc ggagatggcg aaccgctgat attcgtgcat
     2041 gggtcgcttt gcgactatcg ctactggaat gcgcagactg cggcgctgtc gcagcatttc
     2101 cgctgcatct cggtgagcct gagccactac tggccggcca gcgaggcgtg tatccagggg
     2161 gaattcggct ggcagacgca cgtcgccgaa ctggccgagt tcatcgaagc gatcgacctg
     2221 gggcccgtgc atctggtcgg gcattcacgc ggcggctgcg tggcgtttca tgttgcacgc
     2281 gagtatccgc gactcgtgaa gacattgacg ctcgccgatc cgggtggtcc gctgcagatc
     2341 gaggggatgc cggacgcgtc gctgccggcc gcgaccaatg tgctgcgcgc gcgtgttgct
     2401 gagctcatcg agagccagca gatcgacgcg ggactggagc tgttcgtcga ttcggtcagc
     2461 gtgcccggat catggcgcaa gagtccgatt gcgttccgca ccatggcgat cgacaacgcg
     2521 ggcacgctgc ccaagcagct ccgcgacccg ttacctgcct attcccgtga aacggctcgc
     2581 gacgtgaaat gcaggacgtt gctgatcgag ggccagcgca gcccgcggat gttccgcaac
     2641 aacgtcgaaa agctcgcgga atggatcgac tatgcggaaa ggcaaactat cgccggtgcg
     2701 tcgcatggga tgaatgtgtc gaatgccggg gcttttaatc ggcttgtgca cgcgttcgcg
     2761 agtttctgag cgggctcttt cttcggtgcg ctgcatgccg tctgcaaaat cccgcgcgct
     2821 cgtcttgagc ggatacgcgg gatagctgcg gacggcatag gtttgttatt ggccggcggc
     2881 cggcgacacc acgctgggtg tcgccagcgt gttgttgctc ttaggagcgc tggtgccaga
     2941 tgtcgaactg ctgttcgtgc tactcggcag gcccgagttc ggcgacctgg cggccatgcc
     3001 gctatccgag ttggtggtgc ctggcgcgcc gtagccggcc gttgcaccgt tcgtctggtt
     3061 cgtctggttg gccgggcctg cggtgctgcc tgccgaaccg gtgccgctcg agcctgcggc
     3121 cgcctgtgca taagcgccac ccgacagtgt aagtgcggcg agggccgcga ttaatgtgct
     3181 agttgccttg ctcatgacct gtccctcctt gatcggactc gaataaaaga cgcagacgat
     3241 cgccgcctgc ttgaccctga gttcagcaat caccgtgccg cttgctctag ccgtcccacc
     3301 ttcgacatcc cggcacccgc aatagaagtt tctcgagcaa tgagccgcct ccggtagact
     3361 aggttttcga caataatgcc gcgacagatc agacaaaatc tctacaaaaa tactaagaat
     3421 ggaggcagct tgcggacggt cgaataccgc ttttccacca tttggcgcat cgatgcgcca
     3481 gtgcaggaag tctgggccgc aatccaggat tcggcgcgct ggcccgagtg gtggaataac
     3541 gtcgagcgcg tggaccagct agaggccggt tccgatcagg gtgtgggcct cgtacagcgc
     3601 tatacatgga aaggctggtt gccct
//
LOCUS       DATASH010000207         3123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1016586,
            whole genome shotgun sequence.
ACCESSION   DATASH010000207 DATASH010000000
VERSION     DATASH010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1016586"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..175)
                     /locus_tag="VHV99_05750"
     CDS             complement(<1..175)
                     /locus_tag="VHV99_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014973395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HEX3634983.1"
                     /translation="MKTLTVGIGCRLQSSAEQIEAAVRATLGSHAFDEISTIASVDTK
                     AHEAGLLEFCARHA"
     gene            complement(172..1008)
                     /gene="cobA"
                     /locus_tag="VHV99_05755"
     CDS             complement(172..1008)
                     /gene="cobA"
                     /locus_tag="VHV99_05755"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEX3634984.1"
                     /translation="MSRAWLIGAGPGDVELMTLKATRALAQADVILVDDLVNPDVLQF
                     ARANALVVYVGKRGGHPSTPQAGIVAQMLEHLQAGRSVARLKGGDPFVFGRGGEEQQA
                     LHAAGIEVDVINGITAGIAAPAAIGIPVTHRDHAQGVVFVTGHGAGNGEPDWAALAAT
                     RMTLVIYMGMRRLNDIVGALLAAGMSADTACAAIESATRPEQRHVLARLGEFADAVSR
                     AGLGSPAIVVMGGVASLALARDAVNAGTANAGAVNAGAVKAVGAANMANSAGATDASD
                     AQ"
     regulatory      complement(1232..1460)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     regulatory      1540..1820
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1887..2987
                     /gene="cobW"
                     /locus_tag="VHV99_05760"
     CDS             1887..2987
                     /gene="cobW"
                     /locus_tag="VHV99_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CobW"
                     /protein_id="HEX3634985.1"
                     /translation="MQTQMRKIPVTIVTGFLGSGKTTLLRHILQHAGGKRIAVIVNEF
                     GELGIDGEILKGCGIGCDDNGVETEGQLYELANGCLCCTVQEEFFPVMEKLVERREQI
                     DHVLIETSGLALPKPLVQAFNWPQIKNSFTVDAVVTVVDGPAAASGQFADNPVAVDAQ
                     RKADPNLDHESPLHELFEDQLSAADLVILNKTDLLDESHQASVEALIREEIPPQVKIV
                     RAQMGQLDLHTLLGLEAASEETIHLRHDHHGSADDADHHHDEFDSVVVHANVSSREAV
                     IAALQGLVETNTIYRVKGFAALPGAAMRLVIQGVGRRFDSYFDRRWQAGETGEGQQSR
                     FVLIGEDLDQAALQQAFGAALGVVASTRPQQA"
BASE COUNT          586 a         1018 c          980 g          539 t
ORIGIN      
        1 acgcatgacg tgcgcagaat tcgagcaaac cggcttcgtg ggctttggtg tcgaccgagg
       61 caatcgtgct gatttcatcg aaggcatgcg agccgagcgt ggcacgcaca gccgcttcga
      121 tctgctcggc cgagctctgc aagcgacaac cgatgccgac ggtaagtgtt ttcattgcgc
      181 gtccgaagcg tccgtcgctc cggcagagtt cgccatgttg gccgcaccca ccgctttcac
      241 cgcacccgca ttcaccgcac ccgcattcgc agtacccgca ttcacagcat ctcttgccaa
      301 agccagcgac gccacgccac ccatcaccac gatcgccggc gaccccagcc cggcgcgcga
      361 caccgcatcc gcgaactctc cgagccgcgc gagcacgtgc cgttgctcgg gacgcgtggc
      421 cgactcgatc gccgcacacg ccgtatcggc gctcatgccg gccgcgagca acgcgccgac
      481 gatatcgttc agccgccgca tgcccatgta aatcaccagc gtcattcggg tggcggcgag
      541 cgcggcccag tccggttcac cgttgccggc gccgtgcccg gtgacgaaca ccacgccttg
      601 cgcatgatcg cgatgcgtga ccggaatccc gatcgccgcg ggcgctgcaa tcccggcggt
      661 gatgccgttg atcacgtcca cttcaatacc cgccgcgtgc aatgcctgct gttcctcgcc
      721 gccacggccg aacacgaacg gatcgcctcc cttcaaacgc gcgacgctgc gaccggcctg
      781 caggtgctcg agcatctgcg cgacgatgcc ggcctgcggc gtcgacggat gcccgccacg
      841 tttgccgaca tacacgacca gcgcgttcgc gcgcgcgaat tgcagcacgt cgggattcac
      901 cagatcgtcg accagtatca catcggcttg cgcgagagcg cgggtcgcct tgagcgtcat
      961 cagttcgacg tcgccggggc cggcgccgat cagccaggcg cggctcatcg tcgcgtgcat
     1021 ggcgcacgcc atgtcgtgac aagcgggaac ggttgaggct tgatgaggcc ggaggcggcg
     1081 gaaattgagg tcatgcggtg ctacggatta cgcgcgacga acaacgccac tcgccgcgct
     1141 tcccacagga aaacgtcaac ggccgcgcca tgtcgtgctt cagcgccttc aattccgtcc
     1201 ctcgcggatt ccggcggccg gcagcgtgac attcactgcc tccgcgtctg gccggtatcc
     1261 gggctgacgg cctcatgcgt tttgccttcc cgtccggatt cgtcagacag tggcatcaaa
     1321 aacgcatgcg cgatgcgaat gcaccgcagg tcgcttaccg ttgcggggac agcacaggtt
     1381 gagcgcggcg agcgcggcct gtttcccgtt taactgcaca tgcgaacgca tgtgcgggca
     1441 ccaaacgcgc gcatacgata gcacacggca ggccgcgccg gcactgcgga aaggccctgc
     1501 cggcgcccgc gtttccttga cggcacggag ccgcgcgcct aaactcgcac gcactttggt
     1561 gctcgcatgc gcgaccgtgc gcatgcagtt aaacgggaaa cagggtgccc acctgcaatg
     1621 gctcgggtca acctgtgctg cccccgcaac ggtaagcgaa aagcggttcc aggttgatag
     1681 cctggcgccg cggagccggc ttcgatgtcc acgcgcaagg ccgcgtcggc atataaccac
     1741 tgggtgagaa accactcgcc cgggaagggg aagcggcgct ttcgtcagcc cggataccgg
     1801 ccagaatacg aagaacgtcc accgcgggga tgcggtggcg cgttccgcgc cggcgtgccg
     1861 cgcaactcct acggatagac ctcacgatgc aaactcaaat gcgcaagatc cccgtcacga
     1921 tcgtcacggg ctttctcggc agcggcaaaa ccacgctgct gcggcacatt cttcagcatg
     1981 cgggcggcaa gcgcatcgcg gtgatcgtca acgagttcgg cgaactcggc atcgacgggg
     2041 aaatcctcaa gggttgcggg atcggttgcg atgacaacgg cgtcgagacc gaagggcaac
     2101 tgtatgaact cgcgaatggc tgcctgtgct gcaccgtgca ggaagagttt tttccggtga
     2161 tggaaaagct ggtggagcgc cgcgagcaga tcgatcacgt gctgatcgaa acctccggtc
     2221 tcgcgttgcc gaagccgctg gtgcaggcgt tcaactggcc gcagatcaag aatagcttta
     2281 ctgtcgacgc ggttgtcacc gtggttgacg gcccggccgc ggcgagcggc cagttcgccg
     2341 acaatccggt cgcggtggat gcgcaacgca aggccgatcc gaatctcgat cacgaatcgc
     2401 cgttgcacga attgttcgaa gatcaattgt cggcggccga tctggtgatt ctgaacaaaa
     2461 ccgatctgct cgacgaatcg catcaagctt ctgttgaagc cctcatccgc gaagaaattc
     2521 cgccgcaagt gaagatcgtg cgcgcacaga tggggcagct cgatctgcat acgttgctgg
     2581 gcctcgaagc ggcctcggag gaaacgatcc acctgcgcca cgatcaccat ggttcggctg
     2641 acgacgccga tcatcaccat gacgaattcg attcggtggt cgtccacgcg aatgtttcgt
     2701 cgcgcgaggc cgtgatcgcg gcgttgcaag ggctcgtcga aaccaacacg atttaccgcg
     2761 tgaaaggatt cgcggcattg ccgggtgcgg caatgcgtct ggtgatccag ggcgtgggcc
     2821 gtcgtttcga tagctacttc gaccgccgct ggcaagccgg agagacgggc gagggccagc
     2881 aaagccgctt cgtgctgatc ggtgaagacc tcgaccaggc cgcgctgcaa caggcgttcg
     2941 gcgccgcatt gggcgttgtg gcgagcaccc ggccgcaaca ggcttaaccg gtcatgcatc
     3001 tgctgcgcac cacgccgggc ggttttgtcg acgacacgca aggggtgatc cgtatcgatc
     3061 agcggcccgc ggacatcgtc gtgctcagtt cggcggacac gacgctgtcg ttgctggcga
     3121 gcg
//
LOCUS       DATASH010000208         6566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_818261,
            whole genome shotgun sequence.
ACCESSION   DATASH010000208 DATASH010000000
VERSION     DATASH010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6566
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818261"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VHV99_05765"
     CDS             complement(<1..458)
                     /locus_tag="VHV99_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3634986.1"
                     /translation="MTQRIAYVTGGMGGIGTSICQRLHKEGFKVVAGCGPNSPRRVKW
                     LEEQKALGFDFIASEGNVGDWESTKAAFDKVKAEVGEIDVLVNNAGITRDVVFRKMTH
                     EDWTAVIDTNLTSLFNVTKQVIDGMVERGFGRVINISSVNGQKGQFGQTN"
     gene            complement(673..1854)
                     /locus_tag="VHV99_05770"
     CDS             complement(673..1854)
                     /locus_tag="VHV99_05770"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX3634987.1"
                     /translation="MTDVVIVSAARTAVGKFGGSLAKIAAPELGATVIRAVLERAGLK
                     PEQVSEVILGQVLTAGSGQNPARQSLIKAGLPTAVPGMTINVVCGSGLKAVMLAANAI
                     IAGDADIVIAGGQENMSAAPHVLPGSRDGFRMGDAKLVDSMIVDGLWDVYNQYHMGVT
                     AENVAKEYGITREQQDAFAALSQNKAEAAQKSGRFNDEIVPVEIPQRKGEPLRFTTDE
                     FVRHGVTADSLAGLKPAFSKEGSVTAANASGLNDGAAAVLVMSAKKAEALGLMPLARI
                     KAFATSGLDPKVMGMGPVPASRRCLERAGWTPADLDLMEINEAFAAQACAVNQQMGWD
                     TSKINVNGGAIAIGHPIGASGARVLVTLLHEMQKRDAKKGLASLCIGGGMGVALALER
                     A"
     gene            complement(1990..3804)
                     /gene="phaC"
                     /locus_tag="VHV99_05775"
     CDS             complement(1990..3804)
                     /gene="phaC"
                     /locus_tag="VHV99_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HEX3634988.1"
                     /translation="MQQLFDAWMNAWRSLGTPPGGNGMPFPIPQMPQMPQMGLPQMPS
                     FPSMPDFSKLAAGSLPSLSSFAGLNVPNAAIPSERLQKLQADYSREAMELIQQATRSA
                     TRAPELKDRRFSSDAWSSAPAYAFTAAWYLLNARYLQEMVDALETEPKVRERIRFAVQ
                     QWTAAASPSNFFALNPEAQKTLLDSQGESLRQGVMNLLGDLQRGKISQTDESGFVVGE
                     NLANTEGSVVFENELMQLIQYKPRTATVRERPLLIVPPCINKFYILDLQPENSLVAHG
                     LDSGHQVFLISWRNADASIASKTWDDYIGEGVLKAIGTVSKISGREQINTLGFCVGGT
                     MLATALAVAAARGEHPAASMTLLTAMLDFSDTGVLDVFVDEAHVQMREQTIGGKNGGT
                     PGLMRGIEFANTFSFLRPNDLVWNYVVDNYLKGRTPVPFDLLYWNSDSTNLPGPMYVW
                     YLRNTYLENRLREPGALTTCGEPVDLSRIDVPTFIYGSREDHIVPWQTAYASVPLLAG
                     PLKFVLGASGHIAGVINPPAKKKRSYWMLEGDVEALPESSDEWLEQAAEVPGSWWPEW
                     ITWLDQYGGKKVKPRAAAGSAEFPVIEPAPGRYVRQRE"
     gene            complement(4332..5189)
                     /gene="pgeF"
                     /locus_tag="VHV99_05780"
     CDS             complement(4332..5189)
                     /gene="pgeF"
                     /locus_tag="VHV99_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEX3634989.1"
                     /translation="MTLPDLSFADVVQPAWNVPPRVRALVTTRNGGVSEPPFGRWQSG
                     ADRSGGLNLGMKAGDDPAAVATNRARLLNLAGVGEAAWLEQIHGAGIVRAEDALQQIR
                     ASGTPLRADASVTDRPATVCVVMVADCMPVLLCDEAGRAVGAAHAGWRGLAAGIVEQT
                     AERVAELAGVTPASLHAYLGPAIGPSAFEVGADVRDAFMNGVDGAQREATAHAFVTHP
                     QNAGKYLADLAALARLRLQRLGVTRVVGGDLCTVTQRERFYSYRRDRETGRMAALIWL
                     DDQAQTIAD"
     gene            complement(5186..6460)
                     /locus_tag="VHV99_05785"
     CDS             complement(5186..6460)
                     /locus_tag="VHV99_05785"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX3634990.1"
                     /translation="MTRSNTPGAATGAGNSNKDYSVSADPSDALGVDSLDDDLGGDAL
                     AAGPQSLVPTVASRVADETPRSVVVPDELAGERLDKVLARIFPEFSRNRLQSWIESER
                     VRIDGKPAKIRQPVPLGATIELVPDLLPEQLAFTPEPVPLEIVYEDDTLVVINKPAGM
                     VVHPAAGNWSGTVLNGLLYRYGDAAAGLPRAGIVHRLDKETSGLMVVARTLEAQTDLV
                     RQLQARTVKRRYLALVWGNMPEEGTIDAPIGRDPRERTRMAVVTSASGKAARTHFRRV
                     DTAIWQRQPVTAIHCDLETGRTHQIRVHCAHIGHPLLGDPVYGRARGKRSVTPLPDGF
                     ARQALHAWRLGLIHPQTGRTMQWRADAPEDMQVLSAALGLGRDDAGEFDETYYEEDDY
                     DASLGDDADEGDAYDEGDETGEHDEHDKDGHA"
BASE COUNT         1138 a         2213 c         1967 g         1248 t
ORIGIN      
        1 tagttggtct ggccgaattg ccccttctgg ccgttcaccg acgagatgtt gatgacgcgt
       61 ccgaagccgc gctcgaccat gccgtcgatc acctgcttgg tgacgttgaa caggctggtc
      121 aggttggtgt cgatcaccgc cgtccagtct tcgtgcgtca tcttgcggaa cacgacgtcg
      181 cgcgtaatac cggcgttgtt caccagcacg tcgatctcgc cgacttcggc cttgaccttg
      241 tcgaacgccg ccttggtcga ttcccaatcg ccgacgttgc cttcggacgc gatgaaatca
      301 aaaccgagcg ccttctgctc ttcgagccac ttcacgcggc gcggcgagtt cgggccgcaa
      361 cctgcaacga ccttgaagcc ctctttgtgc agccgttggc aaatgctcgt gccgatgccg
      421 cccatcccgc ccgttacgta cgcaattcgc tgtgtcataa accacactcc gtttttcgtt
      481 ttcgaggcgc cgaccggctg cctcttccct cgcctggtgc ttcatctcca ctgccgccgg
      541 gcgaccggcc gacgaggcca ccgctcaccg cgcgccacgt gctgccctgt gactgacgcg
      601 cggcgacctg cttaccacct gccaaaacgc acctaagccc atcaaagccg gcttgagtcc
      661 gctgaagatc cgtcaagcgc gttcgagtgc cagcgccacg cccatgccgc caccgatgca
      721 cagcgacgcc agacccttct tcgcgtcgcg cttctgcatt tcatgcagca gcgtgacgag
      781 gacgcgggcg ccggacgcgc cgatcggatg gccgatcgcg atcgcgccgc cgttcacatt
      841 gatcttcgac gtgtcccagc ccatttgctg attcaccgcg cacgcctgtg cggcaaacgc
      901 ttcgttgatt tccatcaggt ccagatcggc cggcgtccag cccgcgcgtt cgagacagcg
      961 gcgcgaggcc ggcaccgggc ccatgcccat caccttcgga tccagacccg aggtggcgaa
     1021 cgccttgatg cgcgcgagcg gcatgaggcc gagcgcctcg gccttcttcg ccgacatcac
     1081 cagcaccgcc gccgcgccgt cgttcagacc cgacgcattg gccgccgtca ccgagccttc
     1141 cttcgagaaa gccggcttca gacccgccag cgagtcagcc gtcacaccgt gacgcacgaa
     1201 ctcgtccgtt gtgaaacgca gcggctcgcc cttgcgttgc gggatttcga ccggcacgat
     1261 ttcgtcgttg aaacggcccg acttctgcgc ggcttccgcc ttgttctgcg acagcgccgc
     1321 aaacgcgtcc tgctgctcgc gcgtgatgcc gtattccttg gcgacgttct cggccgtcac
     1381 gcccatgtgg tactggttgt agacatccca caggccgtcg acgatcatcg agtcgaccag
     1441 cttcgcgtcg cccatgcgga aaccgtcgcg cgagcccggc agcacgtgcg gtgcggcgct
     1501 catgttttcc tgaccgccgg caatcacgat gtcggcatcg cccgcgatga tcgcattggc
     1561 cgccaacatc accgccttca ggcccgagcc gcacaccacg ttgatcgtca tgcccggcac
     1621 ggccgtgggc agccctgcct tgatcagcga ctggcgcgcc gggttctggc ccgagcctgc
     1681 cgtcagcacc tgccccagaa tgacttcgct gacctgctca ggcttcagac cagcccgttc
     1741 gagtacggcg cgaatcaccg tcgcgccgag ttcgggcgcg gcgatcttcg ccagcgaccc
     1801 accgaattta cctactgccg tacgggcggc cgatacgatc acaacatcag tcatttccat
     1861 atcctccggg cacatcggct gaatacgccg cagcccgtca agttaaaaat cttgttgctc
     1921 ccgcgtcctt gcttacgcga acgcgcattc ataccgccag ataaaccgca ccgtcaggtg
     1981 cgctcacacc tactcccgct gccgcacata ccgtcccggc gccggctcga tcacagggaa
     2041 ttcggcggag ccggccgcgg cgcgcggctt cactttcttg ccgccatact ggtcgagcca
     2101 tgtgatccat tcgggccacc agctgccagg cacttcggcc gcctgctcga gccattcgtc
     2161 ggagctttcc ggcaacgcct cgacgtcgcc ttccagcatc cagtaactgc gttttttctt
     2221 cgccggcgga ttgatcacac ccgcgatatg ccctgacgcg ccgagcacga acttcaacgg
     2281 tcctgcgagc aacggtaccg atgcataggc ggtttgccac ggcacgatat ggtcttcgcg
     2341 cgaaccgtag atgaaggtgg gcacgtcgat cctggagagg tcgaccggct cgccgcaggt
     2401 ggtcaacgca cccggctcgc gcagacgatt ctcgagatat gtgttgcgca gataccagac
     2461 atacatcggc cccggcagat tggtcgaatc gctgttccag tacagcaggt cgaacggcac
     2521 cggcgtgcgg cccttgaggt aattgtcgac gacgtagttc cacaccagat cgttcggccg
     2581 caggaacgag aaggtgttgg cgaactcgat gccgcgcatc aatcccggag tgccgccgtt
     2641 cttgccgcca atggtctgct cgcgcatctg cacgtgcgcc tcgtcgacga acacatcgag
     2701 cacgcccgtg tccgagaaat cgagcatcgc ggtgagcaag gtcatcgacg cagccggatg
     2761 ttcaccgcgc gccgccgcca ctgccagcgc cgtggcgagc atggtgccgc cgacgcagaa
     2821 accgagcgtg ttgatctgct cgcggccgct gatcttgctg accgtcccga tcgccttgag
     2881 cacgccctcg ccgatgtagt cgtcccacgt cttgcttgcg atcgacgcgt ccgcgttgcg
     2941 ccacgaaatc agaaacacct gatgacccga atcgagcccg tgcgcgacca gcgagttctc
     3001 cggttgcaaa tcgagaatgt agaacttgtt gatgcaaggc ggcacgatca gcagtggccg
     3061 ttcacgcacg gtagccgtgc gcggcttgta ctggatcagc tgcatcagct cgttctcgaa
     3121 caccaccgaa ccttcggtgt tcgcaagatt ttcgccgacc acgaagcccg attcgtcggt
     3181 ctgcgaaatc ttgccgcgct gcaagtcgcc cagcagattc atcacgccct gccgcaagct
     3241 ctcaccctgg ctatccagca aggtcttttg tgcttccgga ttcaatgcga agaagttgct
     3301 cggcgatgcg gccgcggtcc attgctgaac ggcgaaacgg atacgctcgc gcactttcgg
     3361 ctccgtttcg agcgcgtcga ccatttcctg cagatagcgc gcgttcaaca gataccaggc
     3421 ggcggtgaat gcgtaggccg gcgctgaact ccacgcgtcg gaactgaagc ggcggtcctt
     3481 gagttcgggc gccctggtcg cggaacgggt ggcctgctgg atcagctcca tcgcctcgcg
     3541 cgaataatcg gcttgcagct tctgcagacg ctccgatgga atcgcggcgt tcggaacatt
     3601 cagtccggcg aatgaagaca gtgacggcaa cgaccctgca gccagtttgc tgaagtcagg
     3661 catgcttggg aatgacggca tctgcggcag acccatttgc ggcatctgag gcatttgcgg
     3721 tatcgggaaa ggcatgccat tgccgccagg cggcgtgccg agcgaacgcc atgcgttcat
     3781 ccaggcgtcg aacaactgct gcatgcccgc ggccggcgtt gcgccgctcg taccggctcg
     3841 ctgcgtctgc tccgtcgagg agtccgtgct gggggaagct gaggaatgtg gagctgccat
     3901 acctgctttt gaccggtaag tccttgcgga cgggttgaga ggcaggttca tgcgcacgtg
     3961 ggcgattgct cgcgacctcg tcacgccctg tccctgtacg aggagctgta aggaacattc
     4021 ttgctcccca tcgcaaggca tgtcaatgct tgttcccgat tttgccgcag tgcgatagtt
     4081 ttttaattat cgttttccct gtgtagcaga tcgcgctttt tgaacagggg tggatgagtg
     4141 ccgccgcaag cgaaaaaagc tcccatattg cgtgggttta tgtgcgttta tccgttcgct
     4201 cgcagacact tttggcggca gatctacgca tgcgcggcga attccggcag tgcagcaaaa
     4261 aagcgtgccg cacctccgaa taaaccctca tacgagggct cgtagacaaa gtcgtagcgc
     4321 cggacgccaa ctcaatccgc aatcgtctgc gcctggtcgt ccagccagat cagcgcggcc
     4381 atgcggccag tttcacggtc gcggcgatag gaatagaaac gctcacgttg cgtgaccgta
     4441 cagagatcac cgccgaccac gcgcgtcacg ccgagccgct gcaagcgcaa acgcgccagt
     4501 gcagcaagat cggccagata tttaccggcg ttttgcggat gcgtaacgaa ggcatgcgcc
     4561 gtggcctcgc gttgtgcgcc atccacgccg ttcatgaagg cgtcacgcac gtctgcgccc
     4621 acctcgaatg cgctgggtcc gatcgccggg ccgagatagg cgtgtaacga tgcgggcgtc
     4681 acgccggcga gctcggcgac gcgctccgcc gtctgttcga cgatgcccgc ggccagccca
     4741 cgccagccag catgagccgc accgaccgcg cgccccgctt cgtcgcacaa cagcaccggc
     4801 atgcaatcgg ccaccatcac gacgcatacc gtggcgggcc gatcggtgac gctggcatcg
     4861 gcgcgtagcg gcgtgccgct tgcgcgaatc tgctgaagcg catcttcggc acgcacgatg
     4921 cctgcgccgt gaatctgttc gagccacgcc gcctcaccga cgcctgccag attcagcagc
     4981 cgtgcgcgat tggttgcgac cgcggcggga tcgtcaccgg ctttcatccc cagattcaga
     5041 ccgccggacc ggtccgcgcc actctgccag cggccgaacg gcggctcgct cacgccgccg
     5101 ttgcgcgtgg tgacgagcgc gcgcacacgc ggcggtacgt tccatgccgg ttgcacgaca
     5161 tcggcaaagc tcagatcggg caaggtcatg cgtgaccgtc cttgtcgtgt tcgtcgtgtt
     5221 cgccggtttc gtcgccttcg tcgtatgcgt caccttcatc ggcatcgtcg cccagcgaag
     5281 cgtcgtagtc gtcttcctcg tagtacgtct cgtcgaactc gcccgcatcg tcgcgcccga
     5341 ggccaagcgc cgccgacagc acctgcatgt cttccggtgc atcggcgcgc cattgcatgg
     5401 tgcggccggt ttgcggatgg atcaggccga gccgccatgc gtgcaacgcc tgacgcgcga
     5461 aaccgtccgg cagcggcgtg accgagcgct tgccgcgtgc gcgtccataa accggatcgc
     5521 ctagcagcgg gtggccgata tgcgcgcaat gcacgcggat ctgatgggtg cgtccggttt
     5581 ccagatcgca atgaatcgcg gtgaccggct gacgctgcca gatcgccgta tccacgcgcc
     5641 ggaaatgcgt gcgcgcggct ttgcccgatg cgctcgtcac caccgccatg cgagtgcgtt
     5701 cgcgcggatc gcggccgatc ggcgcgtcga tcgtgccttc ctcgggcatg ttgccccata
     5761 ccagcgcgag ataacggcgc ttgaccgtgc gcgcctgaag ctggcgcacc aggtcggtct
     5821 gcgcctcgag ggtgcgcgcc accaccatca ggccggaggt ttccttgtcc agccgatgca
     5881 cgataccggc acgcggcaag cctgccgcag catcgccgta gcgatacagc aggccgttca
     5941 gcaccgtgcc gctccagttg ccggccgccg gatgcacgac catgccggcc ggcttgttga
     6001 tgaccaccag cgtatcgtcc tcgtagacga tctcgagcgg gaccggctcg ggcgtgaagg
     6061 ccagttgctc cggcagcaga tcgggcacga gctcgatggt cgcgccaagc ggcaccggct
     6121 gacggatctt tgccggcttg ccgtcgatgc gcacccgctc cgactcgatc cagctttgca
     6181 gccggttacg tgaaaactcc ggaaagattc gcgcgagcac cttgtcgagg cgttcgccag
     6241 ccagttcgtc gggcacgaca acgctgcgcg gcgtctcgtc cgccacgcgg ctcgctacgg
     6301 tcggcaccag gctttgcggg ccggcggcga gcgcgtcgcc gccgagatcg tcgtcgaggg
     6361 aatcgacgcc caacgcgtcg gaggggtcag cgcttacgct ataatctttg ttgctattcc
     6421 cggcaccggt tgcggcgcct ggagtatttg agcgggtcat tgagtctggg tcacctggac
     6481 gtaaagctag actggaaaat gcgagccttg aacaccatca ctaaagcggc gatcaatttg
     6541 gcggccatca agaaggcggc ccggaa
//
LOCUS       DATASH010000209         3820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_619869,
            whole genome shotgun sequence.
ACCESSION   DATASH010000209 DATASH010000000
VERSION     DATASH010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619869"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..2300)
                     /locus_tag="VHV99_05790"
                     /pseudo
     CDS             complement(21..2300)
                     /locus_tag="VHV99_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006046807.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
     gene            complement(3458..>3820)
                     /locus_tag="VHV99_05795"
     CDS             complement(3458..>3820)
                     /locus_tag="VHV99_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEX3634991.1"
                     /translation="KNYDPRAKLMRETCHEVLEELGLHDDPLFKLAMALEKIALEDEY
                     FVSRKLYPNVDFYSGIVQRALGIPTAMFTCIFALARTVGWIAQWNEMIADPEQKIGRP
                     RQLFVGETQRGARPLAKR"
BASE COUNT          731 a         1180 c         1199 g          710 t
ORIGIN      
        1 cctgaaagat gctcccgccg ctaaagaagc gcgaccaccc gcacatcgcc ctgctcggcc
       61 gatgcgacca ccttctcgcc gatgcgccgg aacatgatcg acaccgacga atcgcccacc
      121 ttcagatcgc cgacttcgag ccagtcgatg ccctacggca gcatgggttg ctcgatgacc
      181 acttcgcgcc gggtcgcatc gatcgtgatg cccagacacg cttcgagcat catgaacggt
      241 gaacccgctg cccaggcctg cggcagacac gcgaccggat aggcagtggg cggctcgccg
      301 cgccgacgcg ggaacccaca gaacagttcc ggcagacgca tgtcaaaatt gaccgccgcc
      361 tggaacagcg cctgcaacag ccgcacggcc gccgccttgc cgccataacg cgacaggccg
      421 cgcgcgcaga gcgcgttgtc atgcggccag acggagccgt tgtgatacgc catcgggtta
      481 aagcgcgcct ggctcgcggc cagcgtgcgc acgccccagc cggtgtggaa caaggtggag
      541 tcgagcgcgc gcaccaccgc ttcgccacgc tcgcgcgaag gcaaaccgaa ggccagcaag
      601 tggcccgcat tcgacgccat cacgcgacac aattcgccgt ggccgtcgag cgcgatgccg
      661 tagaacccgg cctcttccat ccagtacttt tcttcgacgc actgacggat tttcttcgcg
      721 cgctcgctgt attgcttcgt ctgatcgtgc aggccgcgca gcttggcgaa gtgagccatt
      781 gtgtcgaagg ccgcgctcgc atacgcctgc acttcgacga gcgcgatcgg gccgtcaggg
      841 aaacggccgt cggcgtggaa gaccgaatcg tggctgtcct tccagccttg attcgcgaga
      901 ccgccatcgg actcgcgctg atagtccagc agaccgtaac gattcttgtc gcagacaccc
      961 gcaacccact gcgccgcgcg ttccaacgcg ggccacaatt cgtcgatcag cgcgaggtcg
     1021 tccgtgcgtg ccgcgtaggc gccggccagc acgataaaca gcggcgtggt gtcgacgccg
     1081 ccgtagtaca gcgcgaacgg cacttcgccg gtggcggcca tttcgccctt gcgcatctcg
     1141 tgcatgatct tgccgggcgc ggcgtcacga aacggcgaat tttcgcgcgc ctgatgttcg
     1201 gcgagaaagc gcaacacgcc cgcggccaac tgcggttgca accacagtgt ctgcaacgac
     1261 gtgatgaccg catcgcgccc gaacggtgtc gagaaccatg ggatgccggc atagggataa
     1321 gggccggtgg cgagatcggt agtcaggaga cccaggtcgg caagcgaacg atcgatccac
     1381 gcgttgaaga gcggattgct cgaacgcacc cgggccgtga cacggcgccg ttcacgcatc
     1441 accaggtggg cgtcgaccag tgcggcgcgc acggcggcgc ggcccacgcg tggacgctcc
     1501 gcatcgattt gcgacagatg ggcttcgctc accgcgtgag tgggctgcgc gacgcctgca
     1561 atcggcttgt tggcttgcgc gactacttgc acggcgactg acagatagat cgacacgcag
     1621 gcttgcgcgg gcagtttgac ggtgtagtcg gcgcgatcgg cgaagagctt gtcgggttcc
     1681 ggcgagaagg cgatctgcac attgcgcgcc acgtcgtcga gaccgatata gccgagcagc
     1741 acctcgcgat tctcgacgcg cgccggttcg acgcggccct gtttgttgcg tttgaggccg
     1801 cgcacttcga acatgtcgcg gaagtcgctg gcgaaggaga tggacagcgg cacgacggcg
     1861 tcgctcgtgc cgtagttggt gagttcgatg gcttcgttga ggacggtgcc cgagagcacg
     1921 cgcacgcgtt cgacgtggat cacgccttcc ggcgtgctgt cgccgccgag cggcggcagg
     1981 gggcgattgg tcagatgcgc ggtgaaggag gtgttgtcgc tgctgacgct gcctgacagc
     2041 aacgagggcg cgcgtccgcc gaaggtgagg cgcaggctcg acagtacgcg ggtgtcgttg
     2101 acgaacaggc cgtcgtcgtg accggtgata tcgccgaggg gatcgttgac gatgaaagtg
     2161 tcgccagact tcagcacgtg ctgggtggcg ctcgcgacgt tgaggttttc cgtcgggatg
     2221 aatgcgccgt ccggctcggg ttcgcggtgg tcgattccgc ccgagtggga caggccgccg
     2281 agggcttctg gctgctgcat cagggtcgct cctatggggt tcgcttgcgc gatggctttc
     2341 agtttcggtt cactttcggt tgctgatggt gatgcctggt cgcttccgat tgtagagggt
     2401 ctgtccgcgt cagacgaact tatcatcgga gagtttcggg ttttttggtg gcacgggacg
     2461 tgcagagggg ttttgctgtt ctgtgcgggt ttgtctgggt gcctgcggtg ttggcctttc
     2521 cttgatttcg gttgctcgcg gcgcgggttt gtctggttgc ctacggcgtt ggcctttcgt
     2581 tgatttcggt tgctcgcggc gtgggtttgt tctgggtgcc tgtggtgttg gcctttcctt
     2641 gaattgatat tggtctatta gcgtcgcccc tgtgcggggc gggcacctac ttttctttgc
     2701 ctgccgcaaa gaaaagtagg caaaagaaag cggctcaccc cgccagctca taagcgggtc
     2761 ccccgcacag ccacggtagt ggtgcatctg gaatcggtgt tctcgcacat tcggcgtgag
     2821 tgacaacgcg ctcatcagct cccactccgc actgcgtgcg tcgcggatgg gtatgcatgg
     2881 gaaaccagtg gcttcgcttc gcgcacgggg agccatcggc ttcgcctcgg cgatgcgcct
     2941 acacgtagcg gcaagccaga agatcgacgg aagcaaaaaa acgatacctg ctgatacacc
     3001 gtgctgaatg cagcgcgagt gcgaaaacgt gatggtcggt tttgtgaagt gcgcttcggc
     3061 gcgcgtagcg ccgccggaag tatgacggct gtgtcactgg cgccgaatgt gcgagggcac
     3121 ggattccaga tgtaccacta ccgcggctgt gcgggggacc cgcttaaaag ttagcggttt
     3181 gagccgcttt cttttgccta cttttctttg cggcaggcaa agaaaagtag gtgcctgccc
     3241 cgcacagggg cgacgctaat agaccactac caattcaagg aaaggccaac accgcaaagc
     3301 aacagacaaa caagcgccac gcgggcaacc agatcaagaa aaagccaccg ccgcgggcgc
     3361 cccgacaaac ccgcgccgcg cggcaaccaa atcaagaaaa ggccaacgcc gtaggcaaac
     3421 gaacaacaag cgccgcacag gcaaaaaacc taaaccttca ccgcttggca agcggccgag
     3481 ccccccgttg agtctccccg acaaacaact gccgcggccg cccgatcttc tgttccggat
     3541 cagcaatcat ctcattccac tgcgcaatcc acccaaccgt ccgcgccaac gcgaagatac
     3601 aggtaaacat agcagtagga atccccaacg cccgctgcac aatccccgaa tagaaatcca
     3661 cattcggata aagcttccgc gaaacgaagt actcgtcctc aagcgcgatc ttctcaagcg
     3721 ccatggcaag cttgaacaac gggtcatcat gcaaccccag ctcttccagc acttcatgac
     3781 acgtctcacg catcagcttc gcgcgcggat cataattctt
//
LOCUS       DATASH010000210         3751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1610871,
            whole genome shotgun sequence.
ACCESSION   DATASH010000210 DATASH010000000
VERSION     DATASH010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1610871"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            384..1181
                     /locus_tag="VHV99_05800"
     CDS             384..1181
                     /locus_tag="VHV99_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016347672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX3634992.1"
                     /translation="MTDAMNLQRLKVTVDGYVARVTLDRPDVRNAFDNATIVELTAAF
                     RALTDDSAIRVIILAANGPAFCAGADLNWMKRMATYSDAENRADALGLATMLNTIYAC
                     NKPVIAQVQGDAYAGGMGLVAVADIAICAESAHFCLSEAKLGLMPATIAPYVIRAIGA
                     RASHRYFVTAERFDAAEALRIGLVHQVVAADALGATVDAIAAAIVANSPNAVRECKQL
                     VVDLADKPIDETLISDTADRIARIRASDEGREGVRSFLEKSKPSWLA"
     gene            1251..2606
                     /gene="bioA"
                     /locus_tag="VHV99_05805"
     CDS             1251..2606
                     /gene="bioA"
                     /locus_tag="VHV99_05805"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013337839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEX3634993.1"
                     /translation="MNKQPKPAPDDWVARSLSAVWHPCTQMKHHERTPLVPIARGKGA
                     WLYDHDGHRYLDAISSWWVNLFGHANPHINAALKNQLDTLEHAMLAGCTHEPAVLLAE
                     RLHALTQHTLGHAFFASDGASAVEIALKMSFHFWRNSGHAEKREFVCLANSYHGETIG
                     ALGVTDVALFKDAYDPLIRHAHVVTSPDARQAREGETARDVAQRALADMKTLFDAKAP
                     QLAAVIVEPLVQCAAGMAMHDPSYLKGLRALCDDYGVHLIADEIAVGCGRSGTFFACE
                     QAAIWPDFLCLSKGISGGYLPLSIVLTRDEIYAAFYDDDMTRGFLHSHSYTGNPLACR
                     AALATLELFDSANVLEENRRKSSVLRAALSPLADHPHVRNLRECGTIFAFDAIIEDPA
                     QARSFSRRFFENALKHELLLRPIGKTVYLMPPYILSDDELTLLAERTRTVFDATLSEA
                     A"
     gene            2603..>3751
                     /locus_tag="VHV99_05810"
     CDS             2603..>3751
                     /locus_tag="VHV99_05810"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016344013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEX3634994.1"
                     /translation="MSITSPALFETLKQGLADIERNGLRRRRRIADSACAAHMTVDGR
                     EIIGFASNDYLGLAAHETLREALAEGAQKYGAGSGGSHLLGGHSRAHAKLEDELAAFS
                     GGFVDQARALFFSTGYMANLATLTTLGALGTSGGRDTLMFCDALNHASLIDGARLSRA
                     EIRIYPHADAEALDAMLDASASKGGTKIIVTDSVFSMDGDIAPLARLLELAERHGAWL
                     VVDDAHGFGVLGPQGRGALAEAALRSPHLVYVGTLGKAAGVSGAFVIAHETVIEWFVQ
                     RARPYIFTTASVPAVAHAVSASLKIIGGQEGDARRAHLATLIDSTRAILKRTRWSPVD
                     SHTAVQPLIIGGNEETLKLTAALDEYRMWVPAIRPPTVPVGTSRLRISL"
BASE COUNT          749 a         1240 c         1086 g          676 t
ORIGIN      
        1 cagagccacg gatgtgtgaa accctccttc tccgaacacc gatacccaca cgcttcgcca
       61 ccagtgactg aacctgtcca aggttcaccc cgcgctatcc gcgaacaacc aacctccaat
      121 agtttccgca gtacagcggc accaccgacc gcatccgccg gacgcaaacg aaaaacctcc
      181 ttccgtgcgc cagcgcgaaa ataacccctg aaccccacca agaccctcca cgataagcga
      241 agttagcaac gtcgccacgc aatggccgct cgcgccccat gcgccacccg aggttcccgg
      301 cctctcaaaa tcgaatcccg cgacgaattt agagataatt ggtgctgagc gaaacaccga
      361 gtcgcgcaca tcgagaaacc cccatgaccg atgcaatgaa tctgcagcgc ctgaaagtca
      421 ccgtcgacgg ctacgtagca cgcgtcacgc tcgatcgccc cgacgtccgc aacgccttcg
      481 acaacgccac catcgtcgaa ctgaccgcag ctttccgcgc gctcaccgac gacagcgcca
      541 tccgcgtcat catcctcgcc gcaaacggcc cggctttctg cgcaggcgcc gacctcaact
      601 ggatgaaacg catggcgacc tactcggacg cagaaaatcg cgccgacgca ctcggcctcg
      661 ccaccatgct caacaccatc tacgcctgca acaaacccgt gatcgcgcag gtccagggcg
      721 acgcatacgc gggcggcatg ggcctggtcg ccgtcgccga catcgcaatc tgcgcggaat
      781 cagcgcactt ctgtctgtca gaagcaaagc tcgggctcat gcccgccacc atcgcgccct
      841 acgtgatccg ggcaatcggc gcgcgcgcat cgcaccgata cttcgtgacc gccgaacgtt
      901 tcgacgccgc cgaagcactg cgcatcggcc tcgtgcatca ggtcgtcgca gcggatgcgt
      961 taggtgccac cgttgatgca atcgccgcgg caatcgttgc caatagcccg aatgcggtcc
     1021 gcgagtgcaa gcaactggtg gtcgatctgg cggacaagcc gatcgatgaa acactgatct
     1081 cggataccgc cgaccgtatc gcgcggattc gcgcctcgga cgaagggcgc gaaggcgtgc
     1141 gcagttttct ggaaaagagc aagccgtcgt ggcttgcctg aatgtattcg ggagcatcgc
     1201 ctgagttcgc ccaagcgcga ctggccgctc cccctggagt cgttttgaac ctgaacaagc
     1261 agccaaagcc tgcacccgat gactgggttg cacgctcgct aagtgccgta tggcacccat
     1321 gcacgcaaat gaaacaccac gagcgcacgc cgctcgtgcc catcgctcgc ggcaagggcg
     1381 cgtggctcta tgaccacgac ggccatcgct atctcgatgc catcagctcg tggtgggtca
     1441 acctcttcgg ccacgcaaat cctcacatca atgccgcact gaaaaatcag ttggacacgc
     1501 tcgaacacgc gatgctcgcc ggttgcacgc atgaacccgc cgtcctgctc gccgagcgtc
     1561 tgcacgccct tacgcagcac acgctgggcc atgcgttctt cgcttccgac ggcgcatcgg
     1621 cagtcgaaat cgcgttgaag atgagcttcc acttctggcg caacagcggc cacgcggaga
     1681 agcgcgagtt cgtctgcctc gcgaacagct atcacggcga gaccatcggc gcgctcggcg
     1741 taaccgacgt cgcgttgttc aaggacgcct acgatccgct gattcgtcat gcgcacgtgg
     1801 tgacatcgcc ggatgctcgc caggcccgcg aaggagagac ggcgcgtgac gttgcgcaac
     1861 gcgcgctcgc cgatatgaaa acactgttcg acgccaaagc cccacagctt gcggccgtga
     1921 tcgtggaacc gcttgttcaa tgcgcagccg gaatggcgat gcacgatcct tcgtatttga
     1981 agggcttgcg cgctttgtgc gatgactacg gcgtacatct catcgccgat gaaatcgcag
     2041 tcggctgtgg tcgcagcggc acgttcttcg catgcgaaca ggctgcgatc tggccggatt
     2101 tcctgtgttt atcgaaggga atcagcggcg ggtatttgcc gttatccatc gtgctgacac
     2161 gcgatgaaat ctacgccgcg ttctacgacg acgacatgac gcgcggtttc ctccactcgc
     2221 attcgtacac gggcaatccg ctcgcctgcc gcgccgccct ggcaacgctc gagctgttcg
     2281 acagcgcgaa tgtccttgaa gagaaccgac gcaaatcgtc cgtgctgcgc gccgcgctaa
     2341 gcccgcttgc agatcatccg catgtcagga acctgcgcga atgcggcacg atattcgcgt
     2401 tcgatgcgat cattgaagac cccgctcaag cacgctcgtt ctcgaggcgt ttcttcgaaa
     2461 atgccttgaa acacgagcta ttgttgcgcc cgataggcaa aaccgtctat ctgatgccgc
     2521 cgtacatcct cagcgacgac gagcttacgc tgctcgccga acgcacgcgc actgtcttcg
     2581 acgccacgtt atcggaggcc gcatgagcat cacatcgccc gctcttttcg agaccctgaa
     2641 acaaggcctt gccgatatcg agcgcaacgg tttacgtcgt cgtcgccgaa tcgccgacag
     2701 cgcctgcgcc gcgcacatga cggtggacgg acgcgagatc atcggctttg cgagcaacga
     2761 ttatctcggg ctggccgcgc acgaaacctt gcgcgaagca ctggcggaag gcgcacaaaa
     2821 atacggcgcg ggcagcggcg gatcgcatct gctcggcggc cattcgcgcg cgcatgcgaa
     2881 gcttgaagac gaactcgcgg cgttctccgg cggtttcgtc gaccaggcgc gcgcgctgtt
     2941 cttcagcacc ggttacatgg cgaatctcgc gacgctgaca acgctcggcg ccttgggcac
     3001 gtcaggcggt cgcgacacgc tgatgttctg cgacgcgttg aaccatgcat cgctgattga
     3061 tggcgcccgt ctgtcgcgtg cggaaattcg catctatcct cacgcagacg ccgaggcgct
     3121 cgacgccatg ctcgatgcat cggcgagtaa gggcgggacg aagatcatcg ttacggattc
     3181 ggtcttcagc atggacggcg atatcgcccc gctcgcgcgg ctgctcgaac tggcggagcg
     3241 tcacggcgcg tggctggttg tcgacgacgc gcacggcttc ggcgtgctcg gtccgcaagg
     3301 ccgcggtgcg ctggctgagg ctgcattgcg ctcaccgcat ctggtgtacg ttggcacgtt
     3361 gggcaaggcg gcaggcgtgt ctggcgcatt cgtgatcgcg cacgaaacgg tgatcgaatg
     3421 gttcgtgcag cgcgcgcggc cgtacatctt tacaacggca tcggtaccgg ccgtggcgca
     3481 cgccgtatcg gcgagtttga agattatcgg tgggcaggaa ggcgacgcaa gacgtgcgca
     3541 tctcgctacg ttgattgaca gtacgcgggc catactaaag cgcacgcgat ggtcgcccgt
     3601 ggattcgcat accgccgttc aaccgttgat tatcggcggc aatgaagaga cgctgaagct
     3661 caccgccgcg ctcgatgaat accgcatgtg ggtgccggcg atccggccgc cgaccgtgcc
     3721 cgtgggcaca tcgcgcctgc gcatttcgct t
//
LOCUS       DATASH010000211         6676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_27112,
            whole genome shotgun sequence.
ACCESSION   DATASH010000211 DATASH010000000
VERSION     DATASH010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6676
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27112"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="VHV99_05815"
     CDS             complement(<1..727)
                     /locus_tag="VHV99_05815"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX3634995.1"
                     /translation="MNELFTPSQRGEATPQPRWPHTGYPAMLTATAAWHVFVLAGWLL
                     TPAAWPWWLAAIFANHAVFTVAGLLPRTSLLGPNWTRLPAGARNADAIALTIDDGPDP
                     AVTPQVLDLLDAFNVRATFFCIGAKAQRYPELAREIVARGHALENHSQVHVHTFSLTF
                     PAALTREIDAAQQTFETLSGERPMFFRAPAGLRNIFLEPVLRKLDLRLAAWTRRGYDT
                     RERDPHVVARRLLDGLAARDILLL"
     gene            964..1728
                     /locus_tag="VHV99_05820"
     CDS             964..1728
                     /locus_tag="VHV99_05820"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3634996.1"
                     /translation="MTGTGIKKVALVTGAGSGIGRASALKLLEHGYSVVLAGRRQAQL
                     DAVVEAAQQLGGDALAVACDVTDADSVAALFDTIRARRGRLDVLFNNAGRGGSPVDID
                     ELDIDEWRSVVDTNLTGVFLCTRAAFGLMKTQTPRGGRIINNGSISAHAPRPNSIAYM
                     ATKHAITGITKAVSLDGRKYDIVCGQIDIGNAATEMAERMARGVPQANGEIAVEPLMD
                     VQHVADAVLHMAELPLSANVQFMTIMASKMPFVGRG"
     gene            1791..1985
                     /locus_tag="VHV99_05825"
     CDS             1791..1985
                     /locus_tag="VHV99_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase-like domain-containing protein"
                     /protein_id="HEX3634997.1"
                     /translation="MPSITPRDDPPPVPPVRPDLDDCCHSGCNPCVFDLYDDALERYQ
                     AALAEWQARQTGHSHTLSPS"
     gene            1982..2851
                     /locus_tag="VHV99_05830"
     CDS             1982..2851
                     /locus_tag="VHV99_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacetylase"
                     /protein_id="HEX3634998.1"
                     /translation="MTDPHLAHIEPFTESHTLDDLHTFVQRYPRLFVLTGAGISTDSG
                     IPGYRDDNGEWKRSPPITLQEFLGTVAMRQRYWARSMVGWPVVAGAQPNAAHTALARL
                     EAAGHVPTLVTQNVDGLHQRAGSREVIELHGGIGDVTCLDCGMQHSRASIQQTLAADN
                     PALLNVTAETAADGDAHLEWHDIESFRVPACTNCGGLLKPAVVFFGESVPRERVEAAS
                     HALDAADAVLVVGSSLMVYSGYRFCVWAHKLGKPIVAINLGRPRADPLLSLKIAAPCA
                     DTLTALAARLALD"
     gene            2957..3598
                     /locus_tag="VHV99_05835"
     CDS             2957..3598
                     /locus_tag="VHV99_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HEX3634999.1"
                     /translation="MTDSHPALRQRSPSAERNREPILAVLREVLPATGCVLEIASGTG
                     QHAICFAGALRGLDWQPSDLDADARASIAAWIAHEGLTNVRAPLALDVHQQDWGVQTL
                     DKLAAVVCINMIHISPWSATEALFAGASRRLADGGVLYLYGPYKRGGAQTSPSNDAFD
                     QQLRSRDPAWGVRDMEAVVALGASVGLVCGEPIAMPANNFSLVFRKHPEAARV"
     gene            3716..5125
                     /gene="gndA"
                     /locus_tag="VHV99_05840"
     CDS             3716..5125
                     /gene="gndA"
                     /locus_tag="VHV99_05840"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014899993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEX3635000.1"
                     /translation="MGKQAIGVVGLAVMGRNLALNIESRGHAVSVYNRSREKTDELIA
                     EYPDKKLVPAFTLEEFVESLEKPRRILLMVKAGEGTDATINQLKPLLEKGDILIDGGN
                     THFTDTIRRNQDLAKSGLHFIGTGVSGGEEGALKGPSIMPGGQRDAYDLVAPILTEIA
                     AKAPDGEPCVAYMGPDGAGHFVKMVHNGIEYGDMQLIAESYAVLKQVVGLSNEELGKV
                     YTEWNQGELDSYLIEITSKIFSKKDEETGKDLVDVILDRAAQKGTGKWTSQNALDLGA
                     PLPLITEAVFARVLSSLKTQRVAASKVLEGPTAKPLGVEREAFIESVRRALYFSKVIS
                     YAQGFAQLRAASEEYKWDLDYGTIAKIFRAGCIIRARFLQKITDAYTKDKAIANLLLD
                     PYFRDIAKNYQSALREVVIAAINAGVPVPAFASAVAYFDAYRSERLPANLVQAQRDFF
                     GAHTFERIDKPGSFHANWS"
     gene            5399..5716
                     /locus_tag="VHV99_05845"
     CDS             5399..5716
                     /locus_tag="VHV99_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4148 domain-containing protein"
                     /protein_id="HEX3635001.1"
                     /translation="MKALISAVVVAAALVAPVASFAQSNQPLTRAEVRAQLVQLEKAG
                     YNPVGDHFDYPANIQAAQARVDAQNGTAQAVNSGYGAPMAGSSQAGRPVSSNDHNSVY
                     FGN"
     gene            complement(5943..6263)
                     /locus_tag="VHV99_05850"
     CDS             complement(5943..6263)
                     /locus_tag="VHV99_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006760526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635002.1"
                     /translation="MNKATFLAVQLNVDDVAASPGLVELLNTHLVFAADVAEAADALV
                     QLASIAGLSSGVEASVEIPAVAIERLREALDNLSAYDESWLLALEPSRALFDEIGRRH
                     RTLH"
     gene            6512..>6676
                     /locus_tag="VHV99_05855"
     CDS             6512..>6676
                     /locus_tag="VHV99_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004190911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HEX3635003.1"
                     /translation="MKIIRSRNFTATRPWGALDIANMRGITTRLHWTDQPYKWHVNDG
                     EEVFAVLDGRV"
BASE COUNT         1288 a         2024 c         2160 g         1204 t
ORIGIN      
        1 gcagcagcag gatgtcacgc gcggcaagac cgtcgagcag ccggcgtgcg accacgtgcg
       61 gatcgcgctc gcgagtatcg tagccgcgcc gggtccacgc cgcgagccgc agatcgagtt
      121 tgcgcagcac cggctcgaga aagatgttgc gcaaacccgc aggggcgcgg aagaacatcg
      181 gccgctcgcc gctcaatgtc tcgaaggtct gctgcgccgc atcgatctcg cgcgtgaggg
      241 ccgccggaaa cgtgagcgaa aacgtatgta cgtgaacctg cgaatggttt tccagcgcat
      301 ggccgcgggc gacgatttcc cgtgcgagct ccgggtaacg ctgtgctttc gccccgatgc
      361 agaagaaggt ggcgcgtacg ttgaaggcgt cgagcagatc gagcacttgc ggtgtgacag
      421 ccgggtcggg accgtcgtcg atcgtcagtg cgattgcgtc cgcgttgcgc gcgccggcgg
      481 gcaggcgcgt ccagttcggg cccagcagcg aggtgcgcgg caacaggccg gccaccgtga
      541 aaacagcgtg attcgcgaag atcgcggcca gccaccacgg ccacgcggcg ggcgtcagca
      601 gccatccggc cagcacgaat acgtgccatg cggcagtggc cgtgagcatc gcgggatagc
      661 cggtgtgcgg ccagcgcggc tgtggtgtgg cttcaccgcg ttgcgacggc gtgaataatt
      721 cgttcataaa gcgtgcttcc tgtcttcctt cctccgtcct gggaaggcct tgaatcgcgc
      781 gcgtgacgcg caacagtgaa cgataccatt gatgtaccgt ccgacgcgga cgctgatacg
      841 aatggaaaac aatgaaagcg cgctgaaaga cgtgacattt tttcggcggc cgcaacgttg
      901 catttgtgat ggacgatccg ctgttgtatg gtgctaagac ccatgaaacg gagaggaaaa
      961 gctgtgacag gaacaggaat caagaaggtg gcgctggtga caggcgcggg cagcggcatc
     1021 ggccgggcca gtgcgctcaa gctgctggag catggctata gcgtcgtgct ggccggccgt
     1081 cgccaggcgc agctcgacgc tgttgtcgaa gcagcgcagc aactgggcgg cgatgcgctc
     1141 gccgtggcct gcgacgtgac ggacgccgat agcgtcgccg cgctgttcga cacgatccgc
     1201 gcgcgacgcg gccggctcga cgtgctgttc aataacgcgg gccgcggcgg ctcgcccgtg
     1261 gatatcgacg aactcgatat cgacgaatgg cgctcggtgg tggacaccaa tctcaccggc
     1321 gtgtttctgt gcacgcgggc cgccttcggt ctgatgaaaa cgcagacgcc gcgaggcggt
     1381 cgcatcatca acaatggctc gatttccgcg catgcgccgc ggcccaacag cattgcctac
     1441 atggcgacca agcatgcgat caccggcatc acgaaagcgg tttcgctcga cggccgcaaa
     1501 tacgacatcg tgtgcgggca gatcgacatc ggcaatgccg cgaccgaaat ggccgagcgg
     1561 atggcgcgcg gcgtgccgca ggcgaacggc gaaatcgcgg tcgagccgtt gatggacgtg
     1621 cagcatgttg ccgacgcggt gctgcatatg gcggaattgc cgctctcggc gaacgtgcag
     1681 ttcatgacca tcatggcgag caagatgccg ttcgtcggca gaggctgagc gcggcgtggg
     1741 agatcaactt atactgaacc ctccccgccg tttcgaaaaa gcgattcgcc gtgcccagca
     1801 tcacacccag ggacgacccg ccgccggtgc cgcccgtgcg gcccgatctc gacgattgtt
     1861 gtcatagtgg ctgcaatccg tgtgtcttcg atctctacga tgacgcgctc gaacgctatc
     1921 aggcagcgct tgccgaatgg caagcgcgcc agacgggcca cagccacacc ctatccccgt
     1981 catgaccgac cctcatctcg cgcacatcga acccttcacc gaatcgcaca cgctggacga
     2041 tctgcatacc ttcgtgcagc gttatccgcg tctgttcgtg ctgaccggcg cgggcatcag
     2101 caccgactcc ggcattccag gctatcgcga cgacaacggc gaatggaagc gttcgccgcc
     2161 gatcacgctg caggaatttc tcggtacggt cgcgatgcgc cagcgctact gggcacgcag
     2221 catggtcggc tggccggtgg tggccggcgc gcagccgaat gccgctcata ccgccttggc
     2281 ccggctcgag gcagcgggtc acgtgccgac gctcgtcacc cagaacgtcg acggtttgca
     2341 tcagcgggcg ggcagccgcg aggtgatcga actgcatggc ggtatcggcg atgtgacgtg
     2401 tctggactgc ggcatgcagc attcgcgcgc gtcgatccag cagacgctgg cggccgacaa
     2461 ccccgcgctg ctgaacgtca ccgccgaaac cgccgccgac ggcgacgcgc atctcgagtg
     2521 gcacgatatc gaatcgttcc gcgttccggc gtgtacgaac tgcggcggtt tgttgaagcc
     2581 cgcagtggtg ttcttcggcg aaagcgtgcc gcgtgagcgg gttgaagcgg cctcgcatgc
     2641 gctcgatgcg gcggatgctg tgctggtggt gggctcgtcg ctgatggttt attcgggcta
     2701 ccgtttctgc gtgtgggcgc acaagctggg caagccgatc gtcgcgatca atctcgggcg
     2761 cccgcgggcc gatccgctgc tgtcactgaa aatcgccgcg ccctgtgcgg atacgctgac
     2821 tgctttggcc gcgcggctgg cgctggattg aggccgtgac ctgcgaccgt gaactatgcc
     2881 cgtgaactac gccgtgaact acgaccgtga acgcgccgaa cgcgtgcgca cgaccctgta
     2941 ttgaaacaaa gtgaccatga ctgactctca tcccgcactg cggcaacgct cgccgtccgc
     3001 cgaacgcaac cgtgaaccga ttctcgctgt attgcgcgag gtgctgccgg ccaccggctg
     3061 tgtgctggaa atcgcgagcg gcacgggcca gcacgcgatc tgcttcgccg gtgcgttgcg
     3121 tgggctcgac tggcaaccga gcgatctcga tgcggatgcg cgtgcgtcga ttgccgcatg
     3181 gatcgcacat gaagggctaa cgaacgtgcg cgcaccgctt gccctcgacg tgcatcagca
     3241 ggattggggc gtgcaaacgc tggataagct cgccgccgtg gtctgtatca acatgattca
     3301 catctcgcca tggagcgcga cagaggcgct gttcgccggc gcgagccgcc gcttggccga
     3361 tggcggtgta ctgtatctgt acggtccata taaacgcggc ggtgcgcaaa cctcgccgag
     3421 caacgatgcg ttcgatcaac aattgcgcag ccgcgacccc gcgtggggcg tgcgcgacat
     3481 ggaagccgtg gtggcgttgg gcgcatcggt cggactcgtg tgcggcgagc cgatcgcgat
     3541 gccggccaac aatttcagtc tggtcttcag gaaacacccg gaggcggcgc gggtctgact
     3601 agcgtccgtc tccgtgcggc aagccggcat ttcttttatc cttgcaccct caacgtcccg
     3661 ccgaggttct ttccggcgtg acgtctcgac attttttgga ctctggagaa ttcacatggg
     3721 caaacaggca atcggcgtgg tggggctagc ggtcatgggc cgcaatctgg cgctcaacat
     3781 cgaaagccgc ggccacgcgg tgtcggtgta caaccgtagc cgtgagaaaa ccgacgaact
     3841 catcgccgaa tatccggaca agaagctggt gccggccttc acgctggaag agttcgtaga
     3901 gtcgctggaa aagccgcgcc gcattctgct gatggtgaaa gcgggcgaag gcaccgacgc
     3961 gacgatcaac cagttgaaac ccctgctgga aaagggcgac attctgatcg acggcggcaa
     4021 cacccatttc accgacacca tccgccgtaa tcaggatctc gcgaagtccg gactgcactt
     4081 catcggcacg ggcgtgtcgg gcggcgaaga gggcgcactg aaagggccat cgatcatgcc
     4141 gggcggccag cgcgacgcct atgacctggt cgcgccgatc ctcaccgaaa ttgccgccaa
     4201 ggcgccggac ggcgagccgt gcgtcgcgta catgggaccg gacggcgcgg gccacttcgt
     4261 gaagatggtg cacaacggca tcgaatacgg cgatatgcag ctgatcgccg agagctacgc
     4321 tgtgctcaag caggtcgtcg gcctgtcgaa cgaagaattg ggcaaggtct acaccgaatg
     4381 gaatcagggc gaactcgaca gctatctgat cgaaatcacc tcgaagattt tcagcaagaa
     4441 agacgaggaa accggcaagg atttggtcga cgtgatcctc gatcgcgccg cgcagaaggg
     4501 cacgggcaag tggaccagcc agaacgcgct cgacctcggc gcgccgctgc cgctgatcac
     4561 ggaggctgta ttcgcgcgcg tgctgtcgtc gctgaagacg caacgcgtgg ccgccagcaa
     4621 ggtgctggaa ggcccgacgg ccaagccgct gggcgtcgag cgtgaggcgt tcatcgagtc
     4681 ggtgcgccgt gcgttgtact tcagcaaggt gatttcgtac gcgcaaggtt tcgcgcaact
     4741 gcgcgcggcc tcggaagaat acaagtggga tctggactac ggcacgatcg cgaagatttt
     4801 ccgcgccggc tgtatcatcc gcgcccgctt cctgcagaag atcacggacg cctatacgaa
     4861 agacaaggca atcgccaatc tgctgctcga tccgtacttc cgcgacatcg ccaagaatta
     4921 tcagtcggcg ttgcgcgagg tcgtgattgc cgccatcaat gcgggcgtgc cggtgccggc
     4981 attcgcctcg gcggtagcgt atttcgacgc gtatcgttcg gaacgcctgc cggcgaatct
     5041 ggtgcaggca cagcgcgatt tcttcggcgc gcatacgttc gagcgtatcg acaagccggg
     5101 cagcttccac gcgaactgga gttgagcttt tgaatcgtgg gccggcgcgc acttgccgca
     5161 aggcgcgctg ggctcactgg gctcactggc gattgaaatc gagacagaga attattgctg
     5221 aaagcaatca gacagttcgc gtgacggtac cgcagcacac ctactattgc gaaattcgaa
     5281 atagagtttc atcgttttag gggtttcccc tagatgctaa cgctcctaaa cttcggttca
     5341 agcgctacgg accacatgtc ctgaagcgga tgaaaaacaa gttctggagg tttacatcat
     5401 gaaagctctt atctctgcag ttgttgtcgc cgcggctctg gtcgctcctg ttgcttcgtt
     5461 tgctcaatcg aaccagccgc ttacccgcgc cgaagtgcgc gctcaactgg tgcaactcga
     5521 aaaggccggc tacaaccccg ttggcgacca tttcgactac ccggccaata tccaggccgc
     5581 gcaggcacgt gtcgatgcac agaacggcac tgctcaagct gtgaatagcg gctacggcgc
     5641 accgatggcc ggcagctcgc aagccggccg tccggtgagc agcaacgacc acaactcggt
     5701 ctacttcggc aactaattgt cgaagctgat gcaagcgccg ccttggtagc aggcggcgag
     5761 ttataaaaaa cgccgaccgg attgccgggc ggcgttttgc cgtttacgcc ccggggcggc
     5821 agccagttcg gtttcaaggc gcgcttcggc gcgcagtctg aacgcgcatg gcggaccggc
     5881 agcacttgcg gacgcgcgcc ggtggtgcgc ggcagtgcgg atgcgtttgc gcaagcgcag
     5941 cgtcaatgca gggttcgatg ccggcggcct atttcatcga atagcgcgcg gcttggttcg
     6001 agcgccagta gccaggattc gtcgtaagcg ctgaggttgt cgagcgcctc gcgcaatcgt
     6061 tcgattgcaa cggcgggaat ctcgacgctg gcttcgacgc cgctcgacag acccgcgatg
     6121 ctcgccagct ggaccagcgc gtcggcggct tcggcgacgt ctgccgcgaa aacgagatgt
     6181 gtgttcagca actcgaccaa accgggcgac gccgccacat cgtcgacgtt gagttgcacg
     6241 gccagaaagg tggctttgtt catccccaac tcctcgcagg attcaggcgt ttgcgtacag
     6301 tgcgtcgcga cagcttgact gagtataggc gagggtcgaa aggattcaat ggtcacacga
     6361 atgcgccggc gcggcctcgc gctagcggtc gtgagcgttg caacacctat aatggggccg
     6421 gtcgaaaagc gtgatcccac cgggccgggc gcttcggtat gcggcatgga tcacgcgcgg
     6481 acagcgtcag cttcccaact cgctattcat catgaagatc atccgcagca ggaacttcac
     6541 cgcaacgcgt ccgtggggcg cgctcgatat cgccaatatg cgcggcatta cgacgcgcct
     6601 gcattggacc gatcagccgt ataaatggca tgtcaacgac ggcgaggaag tgttcgccgt
     6661 gctcgacggc cgtgtg
//
LOCUS       DATASH010000212        25320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1215136,
            whole genome shotgun sequence.
ACCESSION   DATASH010000212 DATASH010000000
VERSION     DATASH010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 25320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25320
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1215136"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..174)
                     /locus_tag="VHV99_05860"
                     /pseudo
     CDS             complement(1..174)
                     /locus_tag="VHV99_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491272.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
     gene            complement(183..1328)
                     /gene="dinB"
                     /locus_tag="VHV99_05865"
     CDS             complement(183..1328)
                     /gene="dinB"
                     /locus_tag="VHV99_05865"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX3635004.1"
                     /translation="MPDDRRIAHIDMDAFFASCELSQYPELRGQAMVVAGRRSDAPRT
                     KPDGTREFSRLRDYTGRGVLTTATYEARALGVHSGMPAMKAARLAPNAILLPVNFDLY
                     RNYSRLFKDAVRSVSPTIEDVGIDEVYADVSQLDDASEKVARNLKSAVFEATKLTCSV
                     GIAPNKLLAKLCSDMQKPDGVTILRLEDLQSRIWPMAAGKINGIGPKASAKLESLGVT
                     TIGEIAARDERWLVEHFGRSYGSWLRRVSHGLDDRPVVTHSEPVSMSRETTFEHDLHA
                     VRHRQELGAAFTRLCEQVALDLQRKGYLARKIGIKLRFNDFQTITRDVTLLEPVADAK
                     ALRNAATQCLKRVELVKSIRLLGVKAGNLQRAGEKKGDESPQFTLDF"
     gene            complement(1617..2147)
                     /locus_tag="VHV99_05870"
     CDS             complement(1617..2147)
                     /locus_tag="VHV99_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013341361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX3635005.1"
                     /translation="MSDAALQALEALQSLQARIAALEAENAVRKTIARYMALCDVPMH
                     TLPGESLAALFTEDAVWEGIGSQYAEKFGRLQGHGPILDMLQRYLPPTPHFTTNVHFL
                     TAEHIAVDGGTAKGRWVMLQASGYVDGKAELISARLEIDFVSPQESTQGHAWLIRHFR
                     TERLFDAPWQVNPRQG"
     gene            complement(2147..2524)
                     /locus_tag="VHV99_05875"
     CDS             complement(2147..2524)
                     /locus_tag="VHV99_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013341360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635006.1"
                     /translation="MTDNRSLPLRVLFCCGVTQNFFDLPREKIGEVWQAYGKMLTEIQ
                     AMESVRVLGVMDDDRLVVGSSDGAPWTFYIMADVADFDTAAAVCNLYRTTPVGEYNLW
                     RYGKIEARVGRALTVPPLDKKDS"
     gene            complement(2597..2983)
                     /locus_tag="VHV99_05880"
     CDS             complement(2597..2983)
                     /locus_tag="VHV99_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011694648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HEX3635007.1"
                     /translation="MPTLEAFLPQGHDEARKAMLIERLTEATVDSIGAPAESVRILLS
                     ELPGANFGIGGKSAAQGAAPMLPVIVAILIAGRTDAQKEALIAALCETAADVLDAPLQ
                     ATRVMIKDVPNTDFGIGGKTARALGR"
     gene            complement(3010..3510)
                     /locus_tag="VHV99_05885"
     CDS             complement(3010..3510)
                     /locus_tag="VHV99_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011660272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HEX3635008.1"
                     /translation="MMNDRNALFSQPTLARAVEFIWREAELLDGRDYTAWLNLWDSSG
                     YYVVPIDPHTTDYAASLNYAYDDHEMREKRVQRMTSGFSASAADAARTVRTVSRFTVS
                     SDAADVAQVKSAQVIVAYKRGVATLFAADLTHTLSFASGEPKIVQKVIRLLDSTEALS
                     AIGFLL"
     gene            complement(3507..4790)
                     /locus_tag="VHV99_05890"
     CDS             complement(3507..4790)
                     /locus_tag="VHV99_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX3635009.1"
                     /translation="MSDTLYQTIDASSLYKRAESDRIAPSLYYDPQLFETELDRIFYK
                     TWIWVAHESELRNPGDFITTTIGRQPVIVVRDKTGAVNVLQNRCRHRGATVCEEHKGN
                     AKGFTCPYHSWSYALDGTLRALPYGDGYEGVCEKGDLPLKKLGVGIYNGLIFASFNES
                     IEPLEDFLGGAKPWIDLFMKQGAGYPIRTNGEHKFKFKGNWKIQLENTTDLYHFPVVH
                     KSWLKSVDDETAKVITSFMTSDEAFCRSLGNGHSLAVLMPEVVDLDKDDGAPLPERFD
                     PLRKKLAESHTPEEVRRIVRSLMGVGFNLNLFPNLALSMAFFRVLRPISAEETEIRHI
                     ALAMDGGPEEANRERLRMHEHFQGPFGFGSPDDAEAWERVQRGSHAGPDVPILVNRGL
                     NRETTADNGEKTAHSTDETGMREAYRQWRAMMEQA"
     gene            4931..5854
                     /locus_tag="VHV99_05895"
     CDS             4931..5854
                     /locus_tag="VHV99_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011660274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3635010.1"
                     /translation="MTSLDHIDLNLLRVFQAIVEERSLTKAGQRLALSQPAVSYALGR
                     LRTLFDDPLFLRTRSGMQPTPVALELSGIVGRALDTVREALRYAESFDPPVSTRTFRL
                     SLSDAGEMAYLPAICKVLHGQAPRVRLSVRPVAGQEIEDALRSSRLDFAIGNHPQLLA
                     STRHQLLFEEDYVCMTRRRAGLPTGGKLSLEHFLAGSHIQVQSLEHSHRALDDALRAQ
                     GVGRNTALEVPHFVALPAILTVTDLYATLPRRLARILNQANAFQIYDLPVKLPVAPVT
                     MHWHEHFHDDDGIKWMRDLLADVVSRFDQPA"
     gene            complement(5951..6925)
                     /locus_tag="VHV99_05900"
     CDS             complement(5951..6925)
                     /locus_tag="VHV99_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HEX3635011.1"
                     /translation="MSIRILVIGAGALGGYFGARLADNGADVTFLLRPERAKQIADSG
                     LVVHSPFGDTRIRAPRTVTSDSLRRLPAHYDVVLVGCKAYDLEDTMDAFEPAVSDATA
                     IVPLLNGIAHLSRLKERFAPSNVLGGFCLISAALDESGQVRHLNDTHQLVFGELDGTM
                     STRVSRLKEAFDGARCEAIATDQIIRRMWEKWVQIAIAAGMTTLMRGSIGAIVSSGGT
                     SFAQALFNECAAVASKAGYPLSEEATARIRSAVADKSSTVTASMMKDLERGARVEADH
                     LIGEMIRIRDAGANGSDPADSTTHGGLSALDLVALHLRVYERRQLEAL"
     gene            7342..8151
                     /locus_tag="VHV99_05905"
     CDS             7342..8151
                     /locus_tag="VHV99_05905"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX3635012.1"
                     /translation="MTTTLKGVSHQNVFAMPYGATGNWGILLPHAGDLDNTGIDYRVN
                     NAVRYVSPSFAGVSLGALYSLGGIPGQMGRDALESFGINYVHGPLSIAAAYTAIDHPA
                     TAVDEGVWTANNPVDGNYGIAAGHYRSTGISAQYVLGKAKLAAVYTNTRFSSLDPTLG
                     AHIGGSVTFNIGELIAAYNITPALQVGGAYSYTEGKVSANGMTPRYHIAGGVADYFLS
                     KSTDVYFQTTWMRAAGDATVVGLAPVIAPSSGKKPVGRARRDSHQVLIVAA"
     gene            8225..9565
                     /locus_tag="VHV99_05910"
     CDS             8225..9565
                     /locus_tag="VHV99_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007732706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3635013.1"
                     /translation="MINMDSKTGSLPAAYSGRFNYVVLLCFFVIVCEGYDAAIMGSIV
                     PALMRYAPWNLNAAEIGAMSSAALLGTLVGCYVVSVISDLMGRRPLLLACVTMFSISM
                     ALAAIAPTPFLFTLARGIGGIGLGGVISASAALTVEFSLPERKNFNFALMYSGYFVGA
                     LLSALVALLFMQQYTWRLVVAVGALPLLYVPALWKYMPESPLYLVSRNRSAEARVIAA
                     SHGLVLENLLAQSESASAGARLSIGKLFGEIFSSRNFIATVAFWVAQVAAVLNLYGIG
                     TWLPQIMRQMGFNLGSSLAFLATFMTGSAIGGIVIGRYADRLGPRKLVLLTYLIGAVC
                     LISLSFKFSIFFTYCLIAVAGLGTGGVAMVQLGLISNYYAAHVRSSAIGWAVGIGRVG
                     AMAGPLVGGFILREGIDVRWNFYIFAAAAVCAGVVVALVPKSAEEVAAGDKPTH"
     gene            9676..10413
                     /locus_tag="VHV99_05915"
     CDS             9676..10413
                     /locus_tag="VHV99_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ethyl tert-butyl ether degradation protein EthD"
                     /protein_id="HEX3635014.1"
                     /translation="MRFTIHLEASVHGDEPLAARPGTTEESFASHAWLKTRRDSLRGL
                     TKAIVHTPVVDDVSDPYHAREHAPDWVLQLYFSELDLLETSCCRDGAIAGLLAYIGEI
                     TKVRYTWTQQTMAARSFGVPEVNSHLTDERASAHCSFLVAYPGPAEDLHIWLRHYIDH
                     HPPIMVRFPNIREAEIFTRVDSPNTLGVGCVDLMQRNKVVFDSPSALNDALSSDVRHE
                     MREDGRRFPPFSGGSSHYAVESVARLY"
     gene            complement(10474..11253)
                     /locus_tag="VHV99_05920"
     CDS             complement(10474..11253)
                     /locus_tag="VHV99_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX3635015.1"
                     /translation="MDNQQTLKRPTRLRNCSEAEWEARVKLAATYRVFYMIGWHELIF
                     NHITLRVRGERDQLLINPFGLAYDEVTASNLVKIDLKGNILSDSDYPVNPAGLTIHSA
                     LHEHVPDAHCVMHIHTTESVAVACMEEGLVDHNIYSSQLHDKVAYHTFEGTTVHNDEK
                     DRMVASLRDKNLLILRNHGLLSHGMTLGHAFVLIWTLNRACEIQVATHSMRGSILPVA
                     SAISERSTKDALQYDPRFRGGDQVLDALIRRLDRQDTSYRQ"
     gene            complement(11423..11938)
                     /gene="hpaC"
                     /locus_tag="VHV99_05925"
     CDS             complement(11423..11938)
                     /gene="hpaC"
                     /locus_tag="VHV99_05925"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HEX3635016.1"
                     /translation="MSATTTQQQFRDAMARLAAAVNVITTDGPGGRCGITASAVCSVT
                     DSPPTMLVCINQTSFVHDVLRENGNVCINVLGAGSQEIARTFAGMTGCSMEERFERTA
                     WQQGMLPVPVLSDAVVRLEGTVDEIKTVGTHSVMFVRIRHIDLHESHDSLVYFARQFH
                     RLPGAVPKQAT"
     gene            complement(11982..13211)
                     /locus_tag="VHV99_05930"
     CDS             complement(11982..13211)
                     /locus_tag="VHV99_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX3635017.1"
                     /translation="MDKALNDMEFAGARIASSDSTVLAENQEPSQGPVTLDALIDEIE
                     RRRDEFDGLSHVPRDMVAKMKRAGIYRASTPRRFGGDALPPPRFLEILERLAIADGST
                     AWVAAFGSANTYLAALPIETQAKIYARGPDQVFAGGLYPPQKAERTEGGFSVSGQWRF
                     ASGCKGADWIGVGIGGVPPTAQNEPDAGKPFTAVFPAKEVEIVDNWNVVGMQGTGSHD
                     LRLKDTFVESDWTFVRGGVALIDEPLYRYPAIAYQAQVHGVCNLGLARAALDLLASMS
                     GATKTTTGAPRLADRAYYRAGLAKAEANLRSARAFFFDAAEQAWATLIAGDPVLPAQA
                     NLLRLSATHAAQTGAEVVMQAYQMAGIGAIYRENRMQRLVRDSMVVTQHAFLGEGVYD
                     AAGALFVGMSPAFAPYP"
     gene            13406..13528
                     /locus_tag="VHV99_05935"
     CDS             13406..13528
                     /locus_tag="VHV99_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635018.1"
                     /translation="MIIGIPIIAEMLNSIPAIHKPVNTVPAASKGIATGRQQAI"
     gene            complement(13706..14848)
                     /locus_tag="VHV99_05940"
     CDS             complement(13706..14848)
                     /locus_tag="VHV99_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX3635019.1"
                     /translation="MKHRRGSGIVLTTLMAAAIVALATPGAYAQCVTSLPAGSIGPKT
                     IVGQGPNGEKAASVDAVKLSDADIAKIKAGKFKVGISMQTMNLDWSQLQVQGITDTLK
                     KYGVEVIGTASAEYQVDKQIADIENTIQRHPDGIISIPVDGTATAATYKKVSEAGIKL
                     VFMDNVPTGLKHPEQYASMISADSEGNGQIAAKVLASCVPQGATVGLVNFGVDYFSTT
                     ERTKAVNDWLKKNRPDIKIKQVAFTDPSKVGQIAGDFLTGNPDVKGLFAVWDQPALDT
                     LTSMRAQGVNIPMTTVDLGLQSAIEIAKGGPLKATGSQRPYDQGVAEATAMMKALIGQ
                     TPPAWIGVQSLPVVQSNVLESYKTVFKKDPPPQLADACKKAKPACG"
     gene            complement(14980..15981)
                     /locus_tag="VHV99_05945"
     CDS             complement(14980..15981)
                     /locus_tag="VHV99_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3635020.1"
                     /translation="MNDSVSPLTAKRQALPSQKSRMRRIAQLLLQGDRPYALYGAFAI
                     LLVVFSFASPWFLSIDNFLNIGRQTALVSIVAIGMTFVIIARQIDLSVGSSLALSGMS
                     AALAMAYVGDNWIIGALAGIGTGAIVGAINGVVTTRLSIPSFLVTLGTLSAARGLALM
                     VTTTRPVIITNDSFIAIFGEGDIAGVPVPIIWTLLAVIAGILLLHYSVFGRQIYAAGG
                     NPTAALYSGINTRRVTTLAFILTGALAGLAALVLSARSHAARPDVVQGLELDVIASVT
                     LGGCSLFGGRGFVLGTLLGSLIIGTLNNGLVLLGVSSSLQLVIKGAIIVAAVAFTRK"
     gene            complement(15978..17615)
                     /locus_tag="VHV99_05950"
     CDS             complement(15978..17615)
                     /locus_tag="VHV99_05950"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEX3635021.1"
                     /translation="MNEPVAVAAASSPQVPLIRVAGVTKRFGGVQALRGVNLEVLPGE
                     VHALLGENGAGKSTLIKILSGVHAYDEGIIEIDGQKVAFDSPARSREAGVAVVYQDLS
                     LVESLSVGANLMLGREPRTRLGFVKNRQLMATVSEFLRSHGIPLDPRTPVGALPFAYR
                     QMTEICKALMGDVRVLILDEPTSALTGGEEQILFDAIHAVTNRGVGVIYVTHRLNEVF
                     RISQRVTVFRDGANAGLFATAQTDMKQLVAAIVGPGHAALQARERTAVGVAGDTSAAG
                     RQTSATSHAAAAPILQLSNVSNDRLHDVDLTVRLGEIHGLAGLIGSGRTEILQTIFGL
                     RTVDAGTLEVDGVSRSRFTPAEAIQLGVALVPEDRHLEGLVLDHSIERNLTLPRLPQF
                     SHWGWLRSQAAVQQAKRSMKELSVKAPGSSTAVKFLSGGNQQKVVFAKWNHPRPKLLL
                     LDEPTVGVDVGAREEIYGVVHDAAQAGTGVLVVSSDLDELLRLCDRISIVADGSIVKT
                     VERAELVNAEALHHLIQLSRSSIESPAAGIAVGANAI"
     gene            17866..18876
                     /gene="deoC"
                     /locus_tag="VHV99_05955"
     CDS             17866..18876
                     /gene="deoC"
                     /locus_tag="VHV99_05955"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEX3635022.1"
                     /translation="MPEAPSLSSSVVALRGGPLIHPDRNPGMPFDASWLDGLRVNQSA
                     VERRTATLATRRTVKKDAQAAWLLKAVTCIDLTTLNGDDTEGRVRRLCAKARQPVRAD
                     LLEALGMAPQGITTAAVCVYHRFVAAAVDALQGSGIPVAAVSTGFPAGLIPHPLKLKE
                     IEASVADGAQEIDIVVTREYVLTGNWQALYDEVREFRAACGPAHLKTILATGDIRTLS
                     NVARASMVCMMAGADFIKTSTGKEGVNATLDVSLVMVRMIREYQERTGVPIGFKPAGG
                     VSSAKSVLAYQILMKEELGRAWLEPELFRIGASSLLADIERQLEHHVSGRYSAFNRHP
                     VA"
     gene            18893..21289
                     /locus_tag="VHV99_05960"
     CDS             18893..21289
                     /locus_tag="VHV99_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX3635023.1"
                     /translation="MSVAEYFSSMEYGPAPEDDQPARAWLAQHEASFGHFIDGAWHAP
                     AAGERFVSHTPATGERLADIAQGDAADIDAAVAAARAAQPHWLALGGAGRARHLYALS
                     RMVQRHSRLFAVLEALDNGKPIRETRDIDIPLVARHFLHHAGWAQLQESEFADYVPLG
                     VVGQIVPWNFPLLMLAWKIAPALATGNCVVLKPAEYTPLTALLFAELAQRAGLPAGVL
                     NVVTGDGRTGAALVEHPQVDKIAFTGSTEVGRLIRAATAGTGKSLTLELGGKSPFIVF
                     DDADLDGAVEGVVDAIWFNQGQVCCAGSRLLVQEGIEARFIAKLKRRMETLRVGTSLD
                     KSIDIGAIVDPVQLERIQSLVETGRREGCAVWQSAETAMPSGGCFYPPTLVTGVAPAS
                     TLAQEEIFGPVLVTMSFRTPDEAIALANNSRYGLAASVWSETIGRALDIAPRLACGVV
                     WVNATNLFDAAVGFGGYRESGYGREGGREGIYEYLKPRAWLNFAERQPVRLKEPPAGT
                     LTRDPLANVTSIDRTAKLFIGGKQVRPDSGYSLQVRAPDGTPVGEVGEGNRKDIRNAV
                     EAARAAQKWSQASTHNRAQVLFYLAENLAVRADEFARQLVVRNGATEAAARAEVDASV
                     ARLFTYAAWADKFDGAVHTPPLRGVALAMHEPLGVIGIACPDEAPLLGFVSLVAPALA
                     MGNRVVVLPSAVCPLTVTDFYQVAETSDVPGGVLNIVTGERGALLPALARHDDVDALW
                     CFGNATDSTLVERESVGNLKRTFVDHGRQFDWFDRSSEGRPFLRQAVQVKNIWIPYGD
                     "
     gene            21465..22427
                     /gene="rbsK"
                     /locus_tag="VHV99_05965"
     CDS             21465..22427
                     /gene="rbsK"
                     /locus_tag="VHV99_05965"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEX3635024.1"
                     /translation="MNMSSQRNGRVVILGIYVTDLTFRAARMPLIGETIAGSAFAMGP
                     GGKGSNQAVAAARAGAEVVFCTRIGNDAFGEIARATWAREGITARASVIDGVSTGAAH
                     IFVDDTTGMNAIIVASGAAGTMNAADVDAIEADIAAASVFVTQLEQPLDAARRGLEVA
                     RRHGVITVFNPAPAMPLDDGIFPLCDYITPNETEATALTGVPIANADDARRAADVLLA
                     KGVGVVIVTLGEGGALLHSATQSVHVPAYHCGRVVETAGAGDGFTGGFAAALARGDDA
                     IAAMRFGCALAGISVTRAGTAPSMPMLDEVIQVLSQSAGVSHTS"
     gene            complement(22562..23287)
                     /locus_tag="VHV99_05970"
     CDS             complement(22562..23287)
                     /locus_tag="VHV99_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEX3635025.1"
                     /translation="MLKPDRLRALADALAKQNVMRLRDAASLLGVSEMTVRRDIAGSP
                     ERFTYLGGYIVSATDVPNNAGYSLEEEKDHFAQAKAEASAIAAKLVVDHETIFIDCGT
                     TLTTLARLIPLERHVTVVCYSLNVAEILRRKPNVRMILLGGVYVPSSDSFAGEESLEM
                     LRRMGINKAFISAGGVDSARGVTCWNFHEVALKQAAMASAVERHLVVDQSKFGVVKAV
                     RFSQVDDFDSIITEKGQEHRKRK"
     gene            complement(23524..24102)
                     /locus_tag="VHV99_05975"
     CDS             complement(23524..24102)
                     /locus_tag="VHV99_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX3635026.1"
                     /translation="MKKQLMLAAGALAAAMSFNAMAADTYQLDPTHTYPSFETDHFGG
                     LSIWRGKFKKSSGTVVLDRVAKTGTVDVTIDMSSVDIGNDKLDAELVTDKFFDAAKYP
                     SATYKGTQIRFDGDKPVEVIGTLTMHGITKPVNLKIESFKCFVNPMLKREVCGTESTA
                     TFNRDDFGVDFGKTYGFKMQTTLHIQAEGIKQ"
     gene            complement(24151..24714)
                     /locus_tag="VHV99_05980"
     CDS             complement(24151..24714)
                     /locus_tag="VHV99_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX3635027.1"
                     /translation="MKSNFYRSILAGVAAFSLFSASLAHADVDTSKSSVIATTKQMNV
                     PVDGKFRKFSAQLNFDPARPAAGSANVSIDTGSYDLGADDYNKQAQGKEWFDSATYPA
                     ATFVSSAIAPAGGNQYKITGKLTIKGKSQTVVVPVTIASQGNTQTFDGALPIKRSQFD
                     VGTGEWKDTSMVADEVVIKFHIVASKK"
     gene            complement(24811..>25320)
                     /locus_tag="VHV99_05985"
     CDS             complement(24811..>25320)
                     /locus_tag="VHV99_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012363122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEX3635028.1"
                     /translation="IALHWLIALLIVCGFYLGWIMTDIPGFTPTKLKYFSWHKWIGVT
                     VFVLAVARVLWRATHRAPALDSATAAWQKAAAHLVHGVLYMLMLAIPLSGYFYSSAAG
                     IQVVYLGIVPLPTFIGPDPALKASLRTVHVLLNYTLLALVAMHVLAALKHQFVDRDGL
                     LARMIPFLK"
BASE COUNT         4717 a         7580 c         7869 g         5154 t
ORIGIN      
        1 tgccgcgcgc cgtatctcga tggcgtcggc ggtcggttcg tccagcgtca ggtcgcgcgt
       61 gaccgtgcgg aagtcgccgt agcgcagctt gatgcccacc gtgcggccaa catagccttt
      121 gcgaaccaga tcttccgcga cccgcacgca caagcgcgta agcgaactcg acagggtacg
      181 tgctaaaaat ccagcgtgaa ctgcggtgac tcgtcgcctt tcttttcccc cgcgcgttgc
      241 aggttgccgg ccttaacccc taacagcctg atggatttga caagttcgac ccgttttagg
      301 cactgagtcg ccgcgttacg caacgccttg gcgtccgcaa caggttccag aagcgtgacg
      361 tcacgcgtta tcgtctggaa gtcattgaag cgcagcttga tgccgatctt tcgcgcaaga
      421 taaccctttc gctgcagatc tagcgccacc tgctcgcaca ggcgggtaaa tgcggcacct
      481 agttcctgcc tgtgccgcac ggcgtgcagg tcgtgctcga atgttgtctc ccggctcatc
      541 gagacaggct cgctgtgtgt caccacgggc ctatcatcaa gaccatgaga gactctgcgg
      601 agccacgatc catagctccg tccgaagtgt tcgacgagcc atcgctcgtc gcgtgcggca
      661 atctcaccga tggttgttac gccgagagac tcaagctttg cgctggcctt cgggccaatg
      721 ccgttgattt tgccggctgc catcggccaa atcctggact gcaaatcctc aagtctcagg
      781 atcgtgacgc cgtctggctt ttgcatgtcc gaacacaact ttgctagaag cttatttggc
      841 gcaattccga ccgaacatgt gagctttgtt gcttcgaata cagcggactt caagttgcgc
      901 gcaactttct ccgatgcgtc atccagctga gagacgtcgg cgtagacctc gtcgattccg
      961 acatcctcga tggtcggact cactgaccgg actgcatcct tgaacagacg cgagtagttt
     1021 cgatacagat cgaagttcac tggaaggaga atggcattcg gcgctaatcg ggccgctttc
     1081 atcgccggca tcccggaatg aactcctagc gctcgcgctt cgtaggttgc ggtcgtcaag
     1141 acgcctctgc ccgtatagtc gcgtaaccgg gagaattcac gggtgccgtc cggctttgtt
     1201 ctcggagcat ccgagcgacg acctgcaaca accattgcct ggcctcgcaa ttcgggatat
     1261 tgcgacagtt cacaggacgc aaagaatgcg tccatgtcga tatgggctat gcgcctatca
     1321 tctggcatgc gttgataaat aactagcagt caatcgtttt gtcgggccaa cgcgacgttc
     1381 gtgaacggcc ttctgacatg attacgcaag ttgcggatct ttagacgaac aacggatgga
     1441 actagtgatg ccaaagagca cgaccggcgc agctactgat gaaacacgtt tgggtagctg
     1501 tcaggtgaca ctgaaaacga tattttgcca gctaagttaa aaattgctga cacggctagt
     1561 gaaaaaatcc gcatggtcaa ggttccatgt gccccctggc ttgcgacgcg cctgtcctat
     1621 ccttggcgtg ggttgacctg ccagggcgcg tcgaagagac gctcggtgcg gaagtgcctg
     1681 ataagccaag cgtggccctg cgtgctttcc tgcggggaca caaagtcgat ttcgagtcgc
     1741 gccgatatca actccgcttt gccatcgaca taaccggacg cctgcaacat aacccagcgg
     1801 cctttcgccg tcccgccatc caccgcaatg tgttcggctg taagaaaatg cacattcgtc
     1861 gtgaagtgcg gcgtgggcgg cagataacgt tgcagcatat ccagaatcgg accatggcct
     1921 tgcaaccggc cgaatttttc ggcgtattga gaaccgatgc cttcccagac cgcgtcttcg
     1981 gtaaagagcg cggcgagcga ttctccgggt agggtgtgca tcggcacatc gcacagcgcc
     2041 atgtaacgcg cgatggtctt gcggaccgcg ttttcagctt cgagcgcggc gatacgggct
     2101 tgcaaagact gcagggcttc gagcgcctgc aaggcagcgt cgctcatcag gaatccttct
     2161 tgtcgagggg cggcaccgtc agcgcacgcc ccacacgcgc ttcgatcttg ccgtagcgcc
     2221 acaaattgta ttcgccaacc ggcgtcgtgc gatacaggtt gcacacggca gccgcagtgt
     2281 cgaagtcggc gacatccgcc atgatgtaga acgtccatgg cgcgccatcg gacgagccga
     2341 ctacgagccg gtcgtcgtcc atcacgccga gtacgcgcac gctttccatg gcttggatct
     2401 ccgtcagcat cttgccgtag gcctgccaca cctcgccgat cttctcgcgc ggcaaatcga
     2461 agaaattctg tgtgacgccg cagcagaaca acacgcgcag cggcaaggat cgattgtcgg
     2521 tcatgcgttg agggtcctta atagtgtgat ggcatgctgc gtcgaaaagg gcagtatgct
     2581 ccggaacgcg ccattattag cggccgagcg cgcgagcggt cttgccgccg atgccgaaat
     2641 cggtgttcgg aacatccttg atcatcacgc gcgtagcctg caatggcgcg tcgagtacgt
     2701 ccgctgcggt ttcgcaaagc gcggcgatca gcgcttcttt ctgcgcgtcc gtgcggccgg
     2761 cgatcaggat cgcgacgatg accggcagca tgggcgctgc gccttgcgcc gcgctcttgc
     2821 cgccgatccc gaagttcgca cccggcagtt cgctcagcag aatgcgcacg ctctcggcag
     2881 gcgcaccgat cgaatcgacc gtggcttcag tgagtcgttc gatcagcatc gccttgcggg
     2941 cttcgtcgtg gccttgcggc aaaaaggctt caagtgtcgg cataagtgtc gtgaagtagc
     3001 gtgcggcgct tacagcagga agccgatcgc gcttagcgct tcggtcgaat cgagcaggcg
     3061 gatcaccttc tgaacgatct tcggctcgcc gctcgcaaaa ctcaatgtgt gcgtcagatc
     3121 cgccgcgaac aacgtggcta cgccacgctt gtatgcgacg atgacctgcg ccgacttcac
     3181 ttgcgcgaca tccgcggcat cgcttgacac cgtgaaccgc gataccgtgc gtacggtacg
     3241 tgccgcatcg gccgcggacg ccgagaaacc ggacgtcatg cgttgcacgc gcttttcgcg
     3301 catctcgtga tcgtcgtagg cgtagttcaa cgaggccgcg tagtcggtcg tgtgtggatc
     3361 gatcgggaca acgtagtagc ccgatgagtc ccacagattc agccatgcag tgtagtcccg
     3421 gccgtcgagc agttcggctt cacgccagat gaattcgacc gcgcgggcta aggtcggttg
     3481 cgagaagagt gcgttgcggt cgttcatcat gcttgctcca tcattgcgcg ccactgcctg
     3541 tacgcttcgc gcatgccagt ttcgtcagtg gaatgcgctg tcttttcgcc gttgtcggcg
     3601 gtcgtttcgc ggttcaggcc gcggttgacg agaatcggta cgtcggggcc ggcatgcgat
     3661 ccacgctgca cgcgctccca ggcttcggca tcatcggggc tgccgaaacc gaacggaccc
     3721 tggaagtgtt cgtgcatgcg cagacgttcg cggtttgctt cttcaggccc gccgtccatc
     3781 gccagcgcga tgtggcgaat ctcggtttct tccgcggaaa tcgggcgcag cacgcggaag
     3841 aatgccatcg acagcgcgag attcgggaac agattcaggt tgaagccaac gcccatcagc
     3901 gagcgcacga tgcggcgcac ttcttcgggc gtgtggcttt ccgcgagctt cttcctgagc
     3961 ggatcgaaac gttcgggcag cggcgcgccg tcgtccttgt cgaggtcgac cacttcgggc
     4021 atcagtacgg cgaggctgtg gccattgccg agcgagcggc agaacgcttc gtcgcttgtc
     4081 atgaagctcg tgatcacctt cgccgtttcg tcatcgacgg acttcagcca cgacttgtgc
     4141 acaaccggga agtgatacag gtctgtcgtg ttttcgagct gaatcttcca gttgcccttg
     4201 aacttgaact tgtgctcgcc attcgtgcgg atcggatagc ccgcgccttg cttcatgaac
     4261 aggtcgatcc acggcttcgc gccacccaga aagtcttcga gcggctcgat cgactcgttg
     4321 aagcttgcga aaatcaggcc gttgtagatg cccaccccca gcttcttgag cggcagatcg
     4381 cctttctcgc agacgccttc gtagccgtcg ccgtacggca gcgcgcgcag cgtgccgtcg
     4441 agtgcatagg accagctgtg atacgggcac gtgaagccct tggcattgcc cttgtgctct
     4501 tcgcataccg tcgcgccacg atggcggcag cggttttgca gcacgttcac cgcgcccgtc
     4561 ttgtctcgca cgacgatcac cggctggcgg ccaatggtcg tcgtgatgaa atcgcccgga
     4621 ttgcgcagct cgctttcgtg ggcgacccag atccacgtct tgtagaagat gcggtcgagt
     4681 tccgtttcaa agagctgcgg atcgtagtac agcgagggcg cgatgcgatc ggactcggca
     4741 cgcttgtaca gcgagctcgc gtcgatagtc tgatagagag tgtcgctcat cgaattctct
     4801 tgggtggtga cgatcagctg cgtaagcagc ggcatgaaaa ggtgtatcgg tagtctcagc
     4861 gtatcccaca catcggtcaa atccatctaa aatggaccgc gcaataaacc ccactaataa
     4921 gagtgcccag atgacctcgc tcgatcacat cgacctgaat ctgttgcggg tcttccaggc
     4981 catcgtggaa gaacgcagcc tgaccaaggc gggccagcgg ctagcgcttt cgcagccggc
     5041 ggtcagctac gcgttgggcc gtctgcgtac gctcttcgac gacccgctgt ttttgcgcac
     5101 gcgctcgggc atgcagccca cgccggttgc gcttgagttg tcgggcatcg ttgggcgcgc
     5161 gctcgatacc gtgcgcgaag cgctgcgtta cgcggaatca ttcgacccgc ccgtgagcac
     5221 gcgtacgttc cgcctgtcgc tctccgacgc gggtgagatg gcgtatctgc ctgccatctg
     5281 caaggtgctt catgggcagg cgccgcgggt tcggctatct gtgcgccccg tcgcgggaca
     5341 ggagattgaa gacgcgttgc gttcgagccg gctggacttt gcgataggta atcacccgca
     5401 actgctggcg agcacacgcc atcagttgct gtttgaagag gactacgtgt gcatgacgcg
     5461 tcgccgggca ggtctgccga ccggaggcaa gctgagtctc gagcactttc tggcgggctc
     5521 acatattcag gttcagtcgc tcgaacatag ccaccgtgcg ctcgacgacg ccctgcgtgc
     5581 gcagggcgtg ggtcggaata cggcgcttga agtgccgcat ttcgtcgcgt tgccagccat
     5641 tctcacggtg accgatttgt acgcgacctt gccgcgccga ctggcacgca tcctcaacca
     5701 ggccaacgcg ttccagatat acgacttgcc ggtgaaattg cccgtggctc cggtcacgat
     5761 gcactggcat gagcatttcc acgacgacga cggcatcaaa tggatgcgcg atcttctcgc
     5821 ggacgtggtg agccggttcg atcaaccggc ttgaggcctg acgagcgggc gccaggtact
     5881 tcggtgccga ctttcacgtc gtcgtgcaat gatcgcgatc cgccgcgcga tgcctgctct
     5941 ttcttgacgg ttaaagtgct tccagctgcc gtcgttcgta cactcgcaag tgcaacgcta
     6001 cgagatcgag cgcagagagg ccgccatgtg tcgtcgaatc ggccgggtca ctcccgttcg
     6061 caccggcgtc cctgatccgg atcatctctc cgatcaggtg atcggcctcc acgcgtgccc
     6121 cgcgttcgag atctttcatc atcgaggcgg tgacggtcga gcttttatcg gccaccgccg
     6181 aacgaattcg cgccgtggct tcttcgctca gtggatagcc cgccttcgac gcaactgccg
     6241 cgcattcgtt gaaaagcgcc tgtgcgaatg aagtacctcc tgacgagacg atggccccga
     6301 tcgatcctcg catcagtgtc gtcatgcccg cggcgattgc tatctggacc cacttttccc
     6361 acatccggcg aatgatctga tcggtggcaa tggcctcgca gcgggccccg tcgaaagctt
     6421 ccttcagacg ggacacacgc gtcgacattg ttccatccag ctctccaaag acgagctgat
     6481 gagtgtcgtt caaatggcgc acctgaccgg actcatccag cgcggcggaa atgaggcaga
     6541 agccacctag tacattggaa ggcgcgaatc tttccttcag gcgggaaaga tgcgcaatgc
     6601 cgttgagcag cggcacgatt gcagtggcgt cactcactgc cggctcaaac gcatccatgg
     6661 tgtcctccag gtcatacgcc ttgcatccga cgagcaccac atcgtagtgc gcgggcaacc
     6721 ttcgcagcga atcactggtc acggtccgcg gagcgcgaat gcgcgtgtcg ccaaatggac
     6781 tgtgaacgac gagccccgag tcggcaatct gcttcgcgcg ttctggacga agcaggaacg
     6841 tgacgtccgc gccattgtcg gcgagacggg cgccgaaata gccacccaat gcacccgcac
     6901 cgatgacaag aatccgtatc gacatctgag aagcgaccct tgataaaccc cgcacctcaa
     6961 cgtcgtgagg taactgcggc gcgtgaaatg gatgggccgg aattcgatgc ttgccgaccg
     7021 gtacgcgtcg agcttagcgc gtgacaagcg gggccgccaa ttctcgccat taatgttgga
     7081 catgaatgaa tttgatgagc gcgcttccgg cttggtcaga taagcacggt ttatttgcca
     7141 tgtgactttt ggttgatttg cctaagaaaa gatgtgcgcc gagacttcgc atcgcgtcac
     7201 gtcacgtcgc gtcacgtcgc gaagtcccct aagcagcgct tcatcccgaa tcgaacagca
     7261 cgatgcgttt cgtgcctcga gtccgagtcg gccaggcagg atctaaaaaa agctaaccgt
     7321 gaaccgatga aaaattaccc catgaccacg accctcaaag gggtctcgca ccagaatgtt
     7381 ttcgccatgc catatggtgc caccgggaac tgggggattc tgctcccgca cgcgggtgat
     7441 ctcgacaaca cgggcatcga ttaccgcgtc aataatgcgg tgcgctatgt ctcgccatcg
     7501 tttgccggcg tatcactggg tgccctgtac agcttgggtg ggataccggg gcaaatggga
     7561 cgcgacgccc tcgagtcttt tggcatcaac tatgtgcacg gaccgctcag tatcgcggct
     7621 gcttacacgg caatcgatca tcccgccacc gcagtagacg agggcgtctg gacggcgaac
     7681 aatccggtcg acggcaacta cggtatcgct gccggtcact atcgttcgac agggatctcg
     7741 gctcaatatg tactgggaaa ggcgaagctt gccgcggtgt acacgaacac tcggttttca
     7801 tcgcttgatc cgacgctggg tgcgcatatc ggcggatcgg tcacattcaa tattggcgaa
     7861 ttgattgccg cctataacat tacccccgcg ctgcaggtgg gcggagctta cagctacacc
     7921 gaaggcaagg tctcggcgaa cggaatgaca ccgcggtatc acatcgctgg cggggtagcc
     7981 gactattttc tttcgaaaag taccgacgtc tattttcaga cgacctggat gcgcgcagcc
     8041 ggtgacgcaa cggtcgttgg acttgcgcct gtgattgccc catcgagcgg caaaaagcca
     8101 gttggtcgcg cgcgtaggga ttcgcaccag gttctgattg ttgctgcgta gtttgcatca
     8161 ccgaccgatg gcgtgctggt tgtcgtcgtc gaaatgtcgt tcctctgaga agaaggtgac
     8221 ctctatgatc aatatggata gcaagaccgg cagtttgcct gccgcttatt caggaagatt
     8281 caactacgtc gtcctgctct gtttcttcgt cattgtgtgt gaaggctatg acgcggcaat
     8341 catgggctcg atcgttccgg ctttgatgcg ctatgcgccg tggaatctga acgcggcgga
     8401 aatcggtgcg atgagcagcg cagcattgct cggcacgctc gtcggatgtt acgtcgtgag
     8461 cgtcattagc gacctgatgg gtcgcagacc cttgctgctc gcgtgcgtca ccatgttttc
     8521 catttcgatg gctctcgctg ccattgcgcc taccccgttt ctcttcacgc tggcccgtgg
     8581 gatcggcggt attgggcttg gaggtgtcat ctcagccagc gcagcgctga cggtcgagtt
     8641 ttcgttgccg gaaaggaaga acttcaactt cgcgctgatg tactcgggct attttgtcgg
     8701 cgcgttgctg tccgccctcg tcgcgctcct gttcatgcag caatacacgt ggcgactcgt
     8761 cgtcgctgtc ggtgcattgc ccttgctgta cgttccggcg ttgtggaaat acatgccgga
     8821 atcgccgttg tatcttgtct ctcgcaaccg ctccgccgaa gccagggtta tcgcagcgtc
     8881 gcacggactg gtgcttgaaa atctgctggc tcaaagtgag agcgcgtcgg cgggcgccag
     8941 attgtcgatc ggcaagctgt tcggcgagat attctcgtcg aggaatttca tcgcaacggt
     9001 ggcattctgg gttgctcagg tcgcagccgt tcttaacctg tatggaatcg gtacgtggtt
     9061 gccacagatc atgcggcaaa tgggcttcaa tctcggatcg agcctggctt tcctcgcaac
     9121 cttcatgacg ggatcggcca ttgggggcat tgtgattggc cggtatgccg atcgtctcgg
     9181 gcctcgcaag ttggtccttc tgacctacct gatcggtgcc gtttgcctga tctcgctgtc
     9241 gttcaagttt tccatctttt tcacgtactg ccttatcgcc gtggcgggac tcggaacggg
     9301 cggcgtggcg atggtgcaat tgggattgat ctcaaactac tacgctgcgc acgttcgctc
     9361 gagcgcgatt ggatgggccg tgggcattgg ccgggtcggc gccatggcgg gtccgctcgt
     9421 cggcggcttc attcttcgcg agggcattga cgtcaggtgg aacttctata tcttcgccgc
     9481 tgccgcggta tgcgcgggcg tggtggttgc attggtgccg aagagtgcgg aggaagttgc
     9541 cgcaggagac aagccgacac attgaggcgt cacgccccag tgcgacgtcc aacccggtaa
     9601 tgcttctcgc gcccgctttt gccactggaa acggtgggcg ctagcgcctg ttcactcttt
     9661 actcgtgggg cctgtgtgag attcaccatt catctggaag cgtcggttca tggcgacgaa
     9721 ccgttagcgg caagacccgg aacgactgaa gaatcgttcg cgagtcatgc gtggctgaaa
     9781 acccgccgag actccttgcg tggactgaca aaagcaattg tacatacgcc cgtcgtcgat
     9841 gacgtcagtg atccctatca cgcccgtgag catgcgcccg attgggtatt gcagctctac
     9901 ttcagtgaac tggatttact cgagacaagc tgctgccgcg acggcgccat cgccgggctt
     9961 ctggcataca taggcgagat cacgaaggtt cgatacacgt ggacgcagca gaccatggcg
    10021 gcgcgtagtt tcggggttcc cgaagtcaac tcccatctca cggatgaaag ggcaagcgcg
    10081 cattgctcat ttcttgtcgc ttatccggga ccggcggagg atctgcacat ctggttgcgc
    10141 cactacattg accatcaccc gccgatcatg gtcaggtttc cgaacattcg cgaagctgaa
    10201 attttcacgc gggttgatag cccgaacacg ttgggcgttg gatgcgtcga cctgatgcaa
    10261 cgcaataagg tggtgttcga tagtccctca gcgctcaatg atgcgctgtc gtccgacgtg
    10321 cgacacgaga tgcgggaaga tggaaggcga ttcccgccat tttctggcgg aagctcgcac
    10381 tacgcggtgg aaagcgtggc gcgtctgtac tgaagatgtc accgcgcgga acccgatcga
    10441 cgtcgcatga catcgatcgg gttcgaggcg cggttactgg cggtagctgg tgtcctggcg
    10501 atcgagccgg cggatcagcg catcgagtac ctggtctccg ccgcggaaac gcggatcgta
    10561 ctgaagggcg tccttcgtcg agcgttcgct gatggcggaa gcgacgggca ggatcgagcc
    10621 tcgcatcgaa tgcgtcgcaa cctgtatttc gcatgcgcga ttgagcgtcc agatcagtac
    10681 gaatgcatga ccgagcgtca ttccatgcga caacagccca tggtttcgga ggatcagcag
    10741 attcttgtcg cgaagggacg ccaccatccg gtctttctcg tcgttatgaa ccgtcgttcc
    10801 ttcaaaggtg tgataagcca ccttgtcgtg caactgggac gaatagatgt tgtgatcgac
    10861 gagaccttct tccatgcacg cgaccgcgac gctttcggtg gtatgaatat gcatgacgca
    10921 gtgtgcatcg ggcacatgct catgcagcgc gctatggatc gtcaggcccg ccggattgac
    10981 cggataatcg ctgtcactca ggatattgcc cttcagatcg atcttcacca ggttcgatgc
    11041 agtaacctcg tcataagcca ggccgaatgg attgatcaac agctgatcgc gttcgccgcg
    11101 tacccgcagc gtaatgtgat tgaagatcag ctcatgccat ccgatcatgt aaaagacccg
    11161 ataggtggcc gccagcttga cgcgggcttc ccattcggcc tcactgcaat tgcgtaggcg
    11221 ggttgggcgc ttcaatgttt gttggttgtc catcgaatta cctcgttgcg ttaatgctgt
    11281 tcagtcgaac ccgcgcacat gtgtcggcgc gggctgtcga ggagccagtt tagtttcggc
    11341 ggatagatcg atcaaatcga tcagatatca gcggtcgtat aagccggatt gatggtccgg
    11401 tcgcgaagga agagttgccg cgtcaggtgg cctgcttcgg cacagcgcct ggaagccgat
    11461 gaaattgacg cgcgaagtag acgaggctgt cgtgcgattc atgcagatcg atgtgacgga
    11521 tacgcacgaa catgaccgag tgcgttccga cggtcttgat ctcatccacc gtgccctcga
    11581 ggcgcacgac cgcgtcggac agcacgggaa ccggcaacat cccttgctgc catgcagtgc
    11641 gctcgaagcg ctcctccatc gaacaaccgg tcatgcctgc gaatgtcctg gcaatctcct
    11701 gagacccggc gccgagcacg ttgatacata cattgccgtt ctcgcgcagc acgtcatgca
    11761 cgaaactcgt ctgattgata cacacaagca tggtcggcgg cgagtcggtt acggagcaca
    11821 cggcgcttgc ggtgatgccg cagcgaccgc ctggcccgtc ggtggtaatg acgttgacag
    11881 ccgcggcgag acgggccatc gcatcgcgga actgttgctg ggtcgtggtg gcgctcatgt
    11941 ctggaggaac cttctctgtg gttttacgta gggttgccgg ctcagggata cggcgcgaaa
    12001 gccggcgaca taccgacgaa aagcgcaccc gccgcgtcgt acacgccttc gccgaggaag
    12061 gcatgctgcg taacgaccat agaatcgcgc acgagacgtt gcatgcggtt ctcgcgataa
    12121 atcgcgccga tgcccgccat ctggtaggcc tgcatgacga cttcagcgcc ggtctgcgcg
    12181 gcgtgtgtgg ccgatagacg cagcagattg gcttgcgcgg gcagcaccgg atcgcccgca
    12241 atcagggtgg cccatgcctg ctccgctgca tcgaagaaga aagcccgcgc actgcgcaga
    12301 ttcgcttcgg ctttggcgag acctgcgcgg taataggcgc gatccgcaag acgcggcgcg
    12361 ccggtcgtcg tcttcgtcgc gccggacatg ctggcgagca ggtccagcgc ggcgcgcgcg
    12421 agaccgaggt tgcagacgcc gtgcacctgc gcctggtaag caatagccgg atagcgatag
    12481 agcggttcgt cgatcaacgc aacgccaccg cgcacgaagg tccagtcgct ctcgacgaac
    12541 gtgtccttca ggcgcaggtc gtgactgccc gtgccctgca tgccgaccac gttccagttg
    12601 tcgacgatct ccacttcttt cgccggaaag acagcagtga acggcttgcc cgcatcgggt
    12661 tcgttctgcg cggtcggcgg cacgccacca atacccacgc cgatccagtc cgcgcccttg
    12721 cagccgctcg cgaaacgcca ttgaccgctg acactgaacc cgccttcggt acgctccgct
    12781 ttttgcggcg gatagagacc acctgcaaag acctggtcgg gaccgcgcgc gtagattttc
    12841 gcctgcgttt cgatcggcag ggcggcgaga taggtgttgg ccgagccgaa tgcggcgacc
    12901 catgccgtgg agccgtccgc gatggcgagc cgctcgagga tctcgaggaa acgcggcggc
    12961 ggcagggcat cgccaccgaa acggcgcggc gtgctcgcgc gatagatgcc ggcccgtttc
    13021 atcttcgcga ccatatcgcg tgggacatgc gacagaccgt cgaattcgtc gcggcgtcgc
    13081 tcgatttcgt cgatgagcgc gtcgagggtg accggtcctt gcgacggctc ctggttttcc
    13141 gcaagtacag tggaatcgga tgacgcaata cgggcgccag cgaattccat atcgtttaga
    13201 gctttgtcca tggcatcggt cggtgttgag gtgtgcaatg aagcgtaaat tagacggcac
    13261 accattcatc gtcaaatgaa ggtcaaaaat ggacggcatg aacggaattc atggggcgtt
    13321 agtcgataga ctgcatcgct gagataaggc gctggttctc tctcaatgag gtcggtggac
    13381 gttcgccact tcggtcacga tgccgatgat catcggcatc cccatcattg cggagatgct
    13441 caactcgatt ccggcgatcc acaagccggt gaataccgtg ccggccgcga gcaaaggcat
    13501 cgcgaccggt aggcaacagg ctatctgaag cagtgctgca cgcagcgacg gttgatgaac
    13561 cagcccgatg gcgtctggat gtggctcaat ctgtcgtcat caagatgcgc gagcgttgtg
    13621 aatagttgga gcgcccacgc tcatccgtgc gccgcaatgg cgcccggacg agcgcgcttc
    13681 gcctggctca tgcgtacgcc tgaggtcacc cgcaagccgg cttcgccttc ttgcatgcgt
    13741 cagcgagctg aggcggcggg tccttcttga acaccgtctt ataagactcg agcacgttcg
    13801 actgaaccac cggcaaagac tgcacaccga tccacgccgg cggcgtctgt ccgatcagcg
    13861 ccttcatcat cgccgtggct tcggccacac cctggtcata cggacgttgc gagccggttg
    13921 ccttgagcgg tccgcccttg gcgatctcga tcgcggactg gagtcccaga tcgaccgtcg
    13981 tcatgggaat attcacgcct tgcgcccgca tcgaggtcag cgtgtcgagc gccggttgat
    14041 cccagacggc gaacaagccc ttgacgtccg gattcccggt caggaaatcg cccgcgatct
    14101 gccccacttt cgacgggtcc gtaaaggcga cctgtttgat cttgatgtcc ggacgattct
    14161 tcttgagcca gtcgttcacg gccttggtgc gttccgtcgt gctgaagtaa tccacgccga
    14221 aattcacgag cccgacggtc gcgccctgcg gcacgcacga ggcgagcact tttgccgcga
    14281 tctggccgtt gccttcgctg tcggcggaaa tcatcgaggc gtattgctcc gggtgcttca
    14341 ggcccgtcgg cacgttgtcc atgaacacga gtttgatgcc ggcctcggac actttcttgt
    14401 acgtggcagc cgttgccgtg ccgtcgaccg gaatggagat gatgccgtcc gggtggcgct
    14461 gaatggtgtt ctcgatatcc gcgatctgct tgtcgacctg gtattccgcc gaagccgtgc
    14521 cgatcacttc cacgccgtat ttcttgagcg tatcggtgat gccttgcacc tgcaattgcg
    14581 accagtcgag attcatggtt tgcatcgaaa tgccgacctt gaatttgccg gccttgattt
    14641 tggcgatgtc ggcgtcgctc aatttcacgg catcgaccga ggccgctttt tcgccgttcg
    14701 gcccttgccc gacgatcgtc ttcggtccga tcgaaccggc cggcaggctg gtgacgcatt
    14761 gcgcgtaggc gcccggcgtt gccagcgcca cgatggccgc cgccatgaga gtggttagaa
    14821 ctattcctga gccacgacga tgtttcatgt ttgttctccg ttggtcttca tgtctgtact
    14881 ccgtctcctg ccatccgcgg aaatgcgcgg gcacattgcg cgctttcctg acatcacgag
    14941 tgatcgacgt gccctcgtcg ctttttgctc gctttctggt cacttcctgg taaaggccac
    15001 ggccgcaacg atgatggcgc ccttgatcac cagttgcagc gatgaactga cgccgagcag
    15061 gaccagcccg ttattgagcg tgccgatgat gagactgccg agcagcgtgc cgagcacgaa
    15121 gccgcgacca ccgaacagac tgcagccgcc gagcgtcacc gaagcgatca cgtcgagctc
    15181 gagtccttgc acgacgtcgg gccgtgccgc atgcgagcgc gcggagagca cgagcgcggc
    15241 gagcccggcc agcgcgccgg tgagaatgaa ggcgagggtg gtgacgcgcc gtgtgttgat
    15301 gcccgagtag agcgcggccg tcggattgcc gccggccgcg tagatctgcc ggccgaacac
    15361 gctgtagtgc agcagcagga tgccggcgat caccgccaac agcgtccaga tgatcggcac
    15421 cggcacgccg gcaatgtcgc cctcgccgaa gatcgcgatg aacgagtcgt tggtgatgat
    15481 cacggggcgc gtggtcgtca ccatcaaggc gagtccgcgg gcggcgctca aggtgccgag
    15541 cgtcacgagg aacgaaggaa tgctcagccg cgtggtcacg acgccattga tcgcgccgac
    15601 gatcgcaccc gtgccgatgc ccgcaagcgc gccgatgatc cagttgtcgc cgacatacgc
    15661 catggcgagt gccgcggaca tgcccgacag cgccaggctc gatcccaccg agagatcgat
    15721 ctgccgcgcg atgatcacga aagtcatacc gatcgcgacg atcgacacga gtgcggtctg
    15781 ccggccgata ttcaggaagt tgtcgatgga caagaaccac ggcgaggcga agctgaacac
    15841 caccagcagg atcgcgaacg cgccgtagag cgcgtagggc cggtcgcctt gcagcaggag
    15901 ttgcgcgatg cgccgcatac ggctcttctg cgagggcaat gcctgccgtt tagccgtcag
    15961 cggcgagaca gaatcgttca tattgcgttg gctccgacgg caatgcccgc ggcgggggac
    16021 tcgatggaag aacgggacag ttgaatcagg tgatgcagcg cttcggcatt cacgagttcg
    16081 gcgcgctcga cggttttgac gatcgagccg tccgcgacaa tcgaaatccg gtcgcacagg
    16141 cgcaggagtt catcgaggtc agaagacacg acgagcacgc cggtgcccgc ctgcgcggca
    16201 tcgtgcacta cgccgtagat ttcctcgcgc gcgccgacgt cgacgccgac agtcggttcg
    16261 tcgagcagaa ggagcttggg gcgcgggtga ttccactttg caaagaccac tttctgctgg
    16321 ttgccgccgg atagaaactt gaccgcggtc gacgagccgg gtgccttgac ggacagctct
    16381 ttcatcgagc gcttcgcctg ctggacggcc gcctggctgc gcagccagcc ccagtgggaa
    16441 aattgcggca ggcgcggcaa ggtcaggttg cgttcgatcg aatggtcgag cacgagccct
    16501 tcaagatgcc gatcctccgg caccagcgcc acgccgagct ggatggcttc ggcgggcgtg
    16561 aaacgcgagc gggatacacc gtcgacctcg agcgtgcctg catcgaccgt tcgaagtccg
    16621 aagatggttt gcagaatctc ggtgcgcccg ctgccgatca agcccgcgag tccgtgaatc
    16681 tcgccaaggc gaaccgtgag gtccacgtca tgaagccggt cattgctgac gttcgataac
    16741 tggaggatgg gcgccgcggc ggcatgggag gtggccgatg tttgccggcc ggcagcagac
    16801 gtatcgcccg caaccccgac cgccgtcctc tcccttgcct gcaacgcggc atgaccggga
    16861 ccgacgatcg ccgcgaccag ctgcttcata tccgtttgcg cggtcgcgaa caggccggcg
    16921 ttggcgccat cgcggaacac ggtgacacgc tgcgaaatac gaaacacctc attcaggcga
    16981 tgggtcacgt agatcacgcc cacgccacgg ttggtgaccg cgtgaatggc gtcgaacaga
    17041 atctgctctt cgccgcccgt cagcgcggag gtgggttcgt cgaggatcag cacgcgcacg
    17101 tcgcccatca acgccttgca gatctcggtc atttgccggt aggcgaaagg caatgcgcca
    17161 acgggagtgc gcggatcgag cggaatgccg tgcgagcgca gaaattcgct gacggttgcc
    17221 atgagctggc ggtttttcac aaagccgaga cgcgtgcgcg gctcgcgccc caacatcaga
    17281 ttggcgccga ccgagagcga ttcgacgagg ctcagatcct gatagaccac cgcgacaccc
    17341 gcctcacgtg atcgcgccgg cgaatcgaac gccactttct ggccatcgat ctcgatgatg
    17401 ccttcgtcgt aagcgtgaac gccgctgagg atcttgatca gtgtggactt gcccgcgccg
    17461 ttttcgccga gcagagcgtg gacttcgccg ggcagcacct cgaggttgac gccgcgcagc
    17521 gcttgcacgc cgccaaatcg cttggtgacg cccgcaacac ggatcaatgg aacctgagga
    17581 gatgatgcgg ccgcgaccgc aaccggctca ttcatctggc atgtctcctt cgtcgataag
    17641 acgaaatttt ttctttgtat gacgctatcg gctaaaaccg tgttgtgatc ttcgcatcat
    17701 gaatatgatg cgtcaataac atggatgcgg ttcgcagggc gttaagcacc tgcttatgtg
    17761 atgattgaac cgtaaagctt gttatgattc tatcaatcgt tgtagcataa gtgggaacaa
    17821 attgccggca gctgttgtgc gctgcgccaa atttccggat gtcccatgcc tgaagcccct
    17881 tccttgtctt cgtcggtcgt cgcgctgcgc ggcggaccgc tcattcatcc ggaccgcaat
    17941 ccgggcatgc cgttcgacgc ctcctggctg gacggcttac gcgtcaacca gtccgcggtc
    18001 gagcggcgca cggccacgct tgccacgcgc cgcaccgtca agaaagatgc gcaggccgcg
    18061 tggctgctca aggccgtcac ctgcatcgac ctcaccacgc tgaatggcga cgacaccgaa
    18121 ggccgcgtgc gccgcctctg cgcgaaagcg cgccagccgg tacgcgcgga tctgctcgaa
    18181 gcgctcggta tggcgccgca aggcatcacg acggccgcgg tctgcgtcta tcaccgcttt
    18241 gtcgcggcgg cggtggatgc actgcaaggc agcggcattc cggtcgcggc ggtatcgaca
    18301 ggatttcccg ccggcttgat tccgcatccg ctgaagctga aagagatcga agcctcggtc
    18361 gcggacggcg cgcaggaaat cgacattgtc gtcacgcgtg aatatgtgct gaccggcaac
    18421 tggcaggcgc tgtacgacga agtgcgcgag ttccgggccg cctgcggccc ggcgcatctg
    18481 aagacgattc tggcgaccgg cgacattcgc acgctctcca acgtggcgcg cgcctcgatg
    18541 gtctgcatga tggccggcgc cgacttcatc aagacgtcga ccggcaagga aggcgtcaac
    18601 gccacgctgg atgtctcgct ggtgatggtg cgcatgattc gcgaatacca ggagcgcacc
    18661 ggcgtgccga tcggcttcaa accggcgggc ggcgtctcga gtgcgaagtc ggtgctggcg
    18721 tatcagatcc tcatgaagga agaactcggc cgcgcgtggc tcgagccgga gctgttccgg
    18781 atcggcgcgt cgagcctgct ggccgatatc gaacgccagc tcgaacatca cgtgagcggc
    18841 cgttactccg ctttcaaccg tcaccccgta gcctgaaccc aagaccgatc ccatgagcgt
    18901 agccgagtat ttttcatcga tggagtacgg tcccgcaccc gaggacgatc aacccgcgcg
    18961 cgcgtggctc gcgcagcatg aggcgagctt cgggcatttc atcgacgggg cgtggcatgc
    19021 gccggctgcc ggcgagcgct ttgtctcgca cacgcccgcc acgggcgaac ggctcgccga
    19081 tatcgcccag ggcgatgcgg ccgatatcga cgcggccgtg gccgccgcgc gcgccgcaca
    19141 gccgcactgg ctcgcgcttg gcggcgccgg gcgtgcgcgg catctgtatg cgctctcgcg
    19201 catggtccag cggcatagcc gcctgtttgc cgtgcttgag gcgctcgaca acggcaagcc
    19261 gatccgcgag acacgcgata tcgatatccc gctcgtggcg cggcacttcc tgcatcacgc
    19321 cggttgggcg cagttgcagg agagcgaatt cgccgactat gtgccgctcg gcgtggtcgg
    19381 gcagatcgtg ccgtggaatt tcccgctgct gatgctcgcg tggaagatcg cgccggcgct
    19441 tgcaaccggc aactgcgtcg tactgaagcc cgctgaatac acgccgctga ctgcgctgct
    19501 gttcgccgag ctcgcgcagc gggccggctt gccggcgggc gtgctgaacg tcgtgacagg
    19561 cgatggccgc acgggcgcgg cgctcgttga gcatccgcag gtcgacaaga tcgctttcac
    19621 cggttcgacc gaagttggcc gcctgattcg cgcggccacg gccggcacgg gcaaatcgct
    19681 gacgctcgaa ctcggcggca aatcaccctt catcgtgttc gacgacgcgg atctcgacgg
    19741 cgcggtcgaa ggtgtggtcg acgcgatctg gttcaaccag gggcaggtct gttgcgcggg
    19801 ctcgcgtttg ctcgtgcagg aggggatcga agcgcgcttc attgcgaagt tgaagcgccg
    19861 catggaaacg ctgcgcgtcg gcacgtcgct cgataaaagt atcgatatcg gcgcgatcgt
    19921 cgatccggtg cagctcgaac gcatccagtc gttggtggaa accgggcggc gcgaaggctg
    19981 cgcggtatgg cagtcggcag agacggcgat gccttccggc ggctgtttct atccgcctac
    20041 gctcgtgacc ggcgtggcgc cggcttccac gttggcacag gaagaaatct tcggaccggt
    20101 gctggtgacg atgagttttc gtacgccgga cgaagccatt gcactcgcga acaattcgcg
    20161 ctatggactc gccgcgagcg tgtggagcga gaccatcggc cgcgcgctcg acatcgcgcc
    20221 gcgccttgca tgcggtgtcg tgtgggtcaa tgcgacgaac ctgttcgacg cggcggtcgg
    20281 cttcggcggg tatcgcgaat cgggttatgg acgcgaaggc ggacgtgagg ggatttacga
    20341 atatctgaaa ccgcgtgcgt ggcttaattt tgccgagcgt cagccggtgc ggctgaaaga
    20401 accgcccgca ggcacactca cgcgcgaccc gttggcgaac gtcacctcga tcgatcgcac
    20461 ggcgaagctg tttatcggcg gcaagcaggt gcgcccggat agcggctact cattgcaggt
    20521 tcgcgcgccg gacggcacac cggtcggtga agtgggcgag ggcaaccgca aagacattcg
    20581 caacgcggtc gaggcggcac gcgcggcgca gaagtggtcg caggcgagca cgcataaccg
    20641 cgctcaggtg ctgttctatc tggcggagaa ccttgccgtt cgcgcggacg agttcgcccg
    20701 ccagctcgtg gtgcgtaacg gcgcgacgga agcggcggcg cgcgcggaag tcgacgcatc
    20761 cgtggcgcgt ctattcacct acgcggcatg ggccgacaag ttcgacggcg ccgtgcatac
    20821 gccgccgctg cgcggcgtcg cgctggcgat gcacgagccg ctcggcgtga tcggcattgc
    20881 ctgtccggac gaggcgccgt tgctcggttt cgtttcgctg gtggcccccg cgctggcaat
    20941 gggcaatcgc gtggtggtgc tgccgagcgc ggtgtgtccg ctcacggtaa ccgattttta
    21001 tcaggtagcc gaaacgtccg acgtacccgg cggcgtgctg aacatcgtga cgggcgagcg
    21061 cggcgcgctg ctgccggcgc ttgccaggca cgacgatgtc gacgcgctct ggtgcttcgg
    21121 caacgcgacg gattcgacgc tggtcgaacg cgagtcggtc ggcaatctga aacgtacctt
    21181 cgtcgatcac ggccgccagt tcgactggtt cgaccgttcg agcgaaggcc gcccgtttct
    21241 gcgtcaggcg gtgcaggtga agaacatctg gataccttac ggagattgac tcacagtcgg
    21301 accgaacggc tgaaccgaac ggctgaacga catgcacgcc aaccagaaaa tggaggagac
    21361 aacgtgctga agcatctgga tctgctggtg aatactgtgg tgctgcgcgt agcgcatgcg
    21421 ccgcacgtcg gctccctgac gaacgcgccg cagggagaac gcgaatgaac atgtcttcgc
    21481 aacgaaacgg gcgtgtcgtc attctcggca tctatgtcac ggacctgacg tttcgcgccg
    21541 cacgcatgcc gctgatcggc gagacgatcg cgggctcggc gtttgcgatg gggccgggcg
    21601 gcaaaggctc gaatcaggcg gtggcggcag cgcgcgccgg cgccgaggtg gtgttctgca
    21661 cccgcatcgg caacgacgcg ttcggcgaga ttgcgcgcgc gacgtgggcg agggaaggca
    21721 ttacggcgcg tgcctcggtg atcgacggcg tgtctacggg ggcggcgcat atcttcgtcg
    21781 acgacaccac cggcatgaac gcgatcatcg tcgcatccgg cgcggccggc acgatgaatg
    21841 ccgccgacgt cgacgcgatc gaagccgata tcgccgccgc gagcgtattc gtcacgcaac
    21901 tcgaacagcc gcttgatgcc gcgcggcgcg ggctcgaagt ggcacgccgg cacggcgtca
    21961 tcaccgtgtt caatccggcg cccgccatgc cgctcgatga cggcatcttc ccgttgtgcg
    22021 actacatcac cccgaacgaa accgaggcca ccgcgctcac cggcgtgccg atcgcgaatg
    22081 ccgacgatgc gcgccgtgcc gccgacgtgc tgctcgccaa aggtgtcggc gtggtgatcg
    22141 tgacgctcgg tgagggcggc gcgttgctgc attcggcgac tcaatcggtg cacgtgccgg
    22201 cgtaccactg tggccgcgtg gtagagaccg ccggtgccgg cgacggcttc acgggtggtt
    22261 tcgcggcggc gctggcacgc ggcgacgacg cgatcgctgc aatgcgcttc ggttgtgcgc
    22321 tcgcggggat ttccgtcaca cgtgcgggca ccgcgccttc gatgccgatg ctcgacgaag
    22381 tgatccaggt attgagccag tcggctggcg tgtctcacac gtcttgagtc cggcgtcccg
    22441 agtcatgtgt cctgagtcac gtgtcctgag tcatgcgccc tgacgcaaag ggtgaaaatg
    22501 gcatgctgaa cggcagctac gcacagctgt gccgacacgc cacgttgcgt ggctagcgca
    22561 gtcatttgcg cttgcggtgt tcctggcctt tctcggtgat gatggaatcg aagtcgtcga
    22621 cttgcgagaa acgcaccgcc ttgacgaccc cgaacttgct ctggtccacg accaggtgcc
    22681 gttccacggc acttgccatg gccgcctgct tgagcgcgac ttcgtgaaaa ttccagcacg
    22741 ttacgccgcg cgcgctatcc acaccgcccg ccgaaatgaa cgccttgttg atgcccatgc
    22801 ggcgcagcat ctccagactt tcctcgccgg caaacgaatc ggatgaaggc acgtagacgc
    22861 cgcctagcag aatcatccgc acattcggct tgcgccgcag aatctccgcg acattcagcg
    22921 aatagcagac cacggtgaca tgacgctcca gcggaatcag gcgtgccagc gtggtgagcg
    22981 tcgtaccgca atcgataaag atcgtttcgt ggtcgacgac gagctttgcc gcaatcgcgg
    23041 atgcctccgc cttggcctgc gcgaaatgat ccttctcttc ttcgagtgag tagccggcgt
    23101 tattggggac gtcggtcgcg ctgacaatat agccgccgag ataggtgaag cgttccggac
    23161 tgcctgcgat atcgcgccgg acagtcatct ccgatacgcc cagcaggctg gcggcgtcgc
    23221 gcaagcgcat tacgttctgc ttggccagcg catcggcgag ggcgcggagg cggtcgggtt
    23281 tcagcatggg ggtcctgtcg cgctgtgtgg gccgcaatgt ggatgagcgt tatgttttgt
    23341 tcgagttgat gagagaatta taacaagagc ttgtacgcgg gggcgagcct ggtttgagtc
    23401 tcgaatacgc ggtaaccctc ataccagcaa caaaaaagcc tttaacccgg tgggctaaag
    23461 gcttgttact gacgcgagcg ttcttaggtg agcgctcgac gcgcgctgcg cggcagcaag
    23521 ctattactgc ttgatgcctt cagcctggat atgcagcgtc gtctgcatct tgaagccata
    23581 ggtcttgccg aagtcgacgc cgaaatcgtc gcgattgaac gtcgcggtcg attcggtgcc
    23641 gcacacttcg cgcttgagca tcgggttcac gaagcacttg aacgattcga tcttcagatt
    23701 gaccggcttg gtgatgccat gcatggtcag cgtgccgatc acttccacgg gcttgtcgcc
    23761 gtcgaagcgg atctgcgtgc ccttgtaagt cgcgctcggg tacttggcag cgtcgaagaa
    23821 cttgtccgtg accagttccg cgtcgagctt gtcgttgccg atatccactg agctcatatc
    23881 gatcgtcacg tccaccgtgc cggtctttgc gacgcggtcc agcaccaccg tgccactgct
    23941 cttcttgaac ttgccgcgcc agatcgacag gccgccgaag tggtcggttt cgaagctcgg
    24001 ataagtgtgt gtcgggtcga gttgatacgt atcggcggcc attgcgttga acgacatggc
    24061 ggcggccagc gcgccggcgg cgagcataag ttgtttcttc aagttcttct ccaggtttca
    24121 gaaagtgttg tgacgcttcg gtgtgtccag ttacttcttc gaagcgacga tgtgaaattt
    24181 gatgacgact tcgtcggcga ccatcgacgt gtctttccat tcgcccgtcc cgacgtcgaa
    24241 ctgtgagcgt ttgatcggca atgcgccgtc gaaggtttgc gtgttgccct ggctggcgat
    24301 ggtgaccggc accacgacgg tctgcgattt gcctttgatg gtgagcttgc cggtgatctt
    24361 gtactgattg ccgccggccg gtgcgatcgc gctcgagacg aaagtcgcgg ccggataggt
    24421 cgcgctatcg aaccattcct tgccttgggc ctgtttgttg tagtcgtccg cgccgaggtc
    24481 gtagctgccg gtgtcgatcg acacgttggc gctgccggcc gccggcctgg ccggatcgaa
    24541 attcagctgc gccgagaact tcctgaattt gccgtcgacg ggcacgttca tctgcttcgt
    24601 cgtggcaatg acgctgcttt tgctcgtgtc gacatcagcg tgcgcaagac ttgcgctgaa
    24661 caaggagaag gcggcgacgc cggccagaat ggagcggtaa aagtttgatt tcatggtggt
    24721 acgcatccta tcgaacccac gcgtgcggat tgcacaccgc tgggaattag attgccaaca
    24781 agccttagcc aatcgtgtgt tacgcgtgtg ttatttgagg aatggaatca tccgcgcgag
    24841 taatccgtcg cgatcgacga attgatgctt gagcgccgcg aggacatgca tggcaacgag
    24901 cgccagcagc gtgtagttga gcagcacgtg gaccgtgcgc aggctcgcct tgagcgccgg
    24961 gtccggcccg atgaaggtcg gcagcggcac gatgccgagg tacaccacct gaatgcccgc
    25021 ggccgagcta tagaagtaac ccgacagcgg aatcgccagc atcagcatgt agagcacgcc
    25081 atgcacgaga tgggccgccg ctttctgcca ggcggcggtc gcgctatcga gcgccggcgc
    25141 gcggtgagtg gcgcgccaca gcacgcgcgc cacggccagc acaaagaccg tcacgccgat
    25201 ccatttgtgc cacgagaagt acttcagctt ggtgggcgtg aagccgggga tatcggtcat
    25261 gatccagccg agatagaacc cgcagacgat cagcaatgcg attagccaat gcagggcgat
//
LOCUS       DATASH010000213        15618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4182215,
            whole genome shotgun sequence.
ACCESSION   DATASH010000213 DATASH010000000
VERSION     DATASH010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 15618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15618
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4182215"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            468..644
                     /locus_tag="VHV99_05990"
     CDS             468..644
                     /locus_tag="VHV99_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635029.1"
                     /translation="MEAFGRHLLTHDSHNYNNSTALSQSVANDSVSFAMIGASAIVRM
                     ANRTIATARGDPSV"
     gene            complement(1061..3376)
                     /locus_tag="VHV99_05995"
     CDS             complement(1061..3376)
                     /locus_tag="VHV99_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX3635030.1"
                     /translation="MSYTPVVPFTHDKADSGAFVGGAPAFQQLVRMIDRVAPTDHPLL
                     IFGPTGSGKELVARRVHAHSLRHDQPFVDVNCGAIPENLVEAELFGHVKGAFTGAGEN
                     RQGLFQQVGKGTLLLDEIGELPLVLQPKLLRVLETRTFRPIGSSTSLHFEGRVVAATH
                     RDLRELAREGLFREDLFYRLAVFVLAVPGLEQRIEDIPALANHFASQHARRLEFSPAA
                     LRRLSQHAWPGHIRQLRNLISQLSVLAENTLIDVDSLEPFLANETGAPISRASLADML
                     LQLEGRDKLAAAEDLLIDRALERTGRNKSAAATLLGVGRKTIERRLKSRDEHHQEARK
                     CLDHANALIEESKFAQAIPQLRRCLELLQTCHEQETARRLQFDAYRLLGMSLRSVHGW
                     LYAEATACYAAALAVGEGVCAPAEIAAIQFGIWTTQLTTLQLKQARATAQNMLERAQN
                     GGDRVSLDEAHVAMTNTLFWLGDSEEALACLARGNLLGVAREDTRTGSQGIDLASLAL
                     TFEGLAAYQIGAFAQARRAMEMLILRASEPGAHAFAHAVNLQGAAWLACLFEDVDRLG
                     HLASELESVSIANGFAFYRGVGQILRACHLSSRGHSDEAEAVMLDGFDNHVVCNGGAL
                     FYSFKAWQQGELLLRAGRAKACEAILDAAIDETLARQERVYLGELLVTRARAKWALGE
                     VNAAELGLRTALSTALAFGSVPARVDAARYLADLLRSTGRIAEAIDTLERGLRSLTAD
                     PTTRFTRAMGLLAELRDASFDLDSNGIVNGL"
     gene            complement(3715..4785)
                     /locus_tag="VHV99_06000"
     CDS             complement(3715..4785)
                     /locus_tag="VHV99_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017331089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX3635031.1"
                     /translation="MIFRQLFDQQSSTYTYLLADSTTREAVLVDPVFEQVRRDAALIE
                     ELGLHLLYTIDTHVHADHVTGAWMLNRRTGSRIAISAASGAEGADRYLSEGDKVEFGA
                     RYLAIRATPGHTDGCITLVLDNESMAFTGDCLLIRGTGRTDFQRGDAHAMFRAVHNHI
                     FTLPAACLLYPAHDYRGLTVTSVDEERRFNPRLGGELCESDFTGYMRNLHLPHPKQID
                     VAVPANLKCGWAASYPARMAEPDRAPLTCSFAGIWEINPQWLEENLRAVEIVDVREPD
                     EFNGPLGRIPAATLMSLGELAGRAAELTKERPIVTVCRAGGRSAQATIMLRQAGFERV
                     ANLAGGMLRWRAEGRVVENGSM"
     gene            complement(4829..5485)
                     /locus_tag="VHV99_06005"
     CDS             complement(4829..5485)
                     /locus_tag="VHV99_06005"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013337690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX3635032.1"
                     /translation="MSIRLGEDAPDFTAETTEGTIRFHEWIGDHWAVLFSHPKDFTPV
                     CTTELGYLAGLKPEFDKRNTKIIGLSIDPVSDHQEWVKDIAETQGHEVGYPLIGDPDL
                     TVAKLYDMIHPEASGGGPRTAVDNATVRSVFLIGPDKKVKAMLVYPMSAGRNFDEVMR
                     LLDALQLNAKHAVATPVNWKPGEDVIIPTSVSNDAAMQKYPQGFKTVKPYLRYVEQPK
                     "
     gene            complement(5872..6717)
                     /locus_tag="VHV99_06010"
     CDS             complement(5872..6717)
                     /locus_tag="VHV99_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3635033.1"
                     /translation="MKANTGSSYITTADGTEIFYKDWGTGQPLVFHHGWPLSADDWDA
                     QMMFFLAHGYRVIAHDRRGHGRSTQTVSGNDMDTYAADVAELVKALDLKDAVHIGHST
                     GGGEVTRYVARHGTGNGRVAKAVLISAIPPLFIKSEKNPEGVPREVVDQIRNGTANHR
                     SQFYKDITIPFYGFNRPGATISEGIQENWWRQGMMGGIKAQYDCIQVLSETEFYDDLA
                     IIDVPVLVMHGEDDQICPFATTGAQSVKLLKHGTLKSYPGLPHGMPTTHAEQINADLL
                     AFITA"
     gene            complement(6990..7793)
                     /locus_tag="VHV99_06015"
     CDS             complement(6990..7793)
                     /locus_tag="VHV99_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3635034.1"
                     /translation="MWLAGCAALDPNAHADALANAAGLKREAVRTDRFVLTAFTRITR
                     PDRPLAVYIEGDGLAWISRSEPSLDPTPHQATGLALAAADPEPNVVYLARPCQFTPMA
                     ANPDCGIPYWTGKRFAPEVVHSLDQALDRFAARVPGQRIDLVGYSGGGALAVLIAARR
                     SDIGSIRTVAGNLDTAFVNRLHDVSPMPDSGNPIDVATRVATIPQIHFSGSADTVVPP
                     AIGQRFIAAAGGHCAATRSVANVAHEGDWARLWPMLLHDVPTCPEETTR"
     gene            complement(8017..11535)
                     /locus_tag="VHV99_06020"
     CDS             complement(8017..11535)
                     /locus_tag="VHV99_06020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HEX3635035.1"
                     /translation="MQKKQIILAVLGALAVFEQSHVWAKPGPASFEVPNGTTSTTAQT
                     LGTGGANGTVDAGGSLSISGSTVAITVTGNANIVNNGTITQTGSGRAIRDNTGGLSLT
                     VTNGVGATIQTADADVIQMNEPNSNVTFYNSGTLTSLNASAGGSQAIDFNAITTGSNT
                     LYNFGTGVIQANEADAVRPGVNGFVYNDGIIRSTNNPGSTDSSDGVDLQSNSGVTIVN
                     ATTGTATSAGTGLIEGARHGITGGNTDVTTDGTFTLSVTNNRGGTIQGDNGSGINIDG
                     FNGKEVVTVVNNGTITGNGVTGDGDGVDVDGLVNLTNTGTIKSLHAYNDASEGVTVGG
                     GTIVNSGLIEGSNSATNADGTANTGIGRGITLAGIDKDPTTDAPIPIQGIYGNTTVTN
                     SGLIKGDTDSGIAATGAATAYSLTIVNLAGGVIEGGGATAAAINTGAQNSTVIDYGTI
                     TADSSGRAVDLGSGNSSLQILGGTAVINGGISGGTGTSTLTIVPGTGNSFTYGGTISN
                     FASVSIGTGTVTLFGANTYSGATTLSDGMLVLGNSRALGTGMLNTANSTIAYLGGINL
                     ANAVTLGSNTTLEVDGSNSASQSGAIGESGGSFGIAKTGTGTLVLNGANTFTGASTVQ
                     AGTLQVGSNAGSTASLASSVQVASGGTLAGYGTIKGNVDNSGVMQPGSGGALSVTGNY
                     TQSADAALRVTVGNGAVATGNESSDSGYGRLVVTGGATLAAGSSVQLVKSDSYAFAPG
                     QRFVVVDASANGTNYNTDTLDYSALGYSGLLFGTDVNANGRSELVVNLAGQPASSLAA
                     TPNANAALSGIAKYSGVSPALLNLYNAATALSLGSSAAATRAGTQLSPTAQASLSRAA
                     AAPTLDALNVVSAHADSLRLAQANGATGISTGEAAPASGVWGQAFGGHAGQGELDGVD
                     GYSANYGGLLVGVDRSVSDNWRAGGVFSYTNTAVNNTGNSAGDSTRVNAYGLIGYASY
                     SGKPWYVNLAAGAVQQRYDSTRVVDFTGFSGVANGQFNGQQYVASAEFGYPLAFGAVT
                     LTPLASLAYSYLHQGSYTESGGNGAALSVGATHATSVRSNAGLKLEKGFASQYGDIIP
                     YLQMQWTHEYDHTRLATGASYAADPTGSTAFTTVGATPVADLADIQLGVTLLRASNLS
                     LSALYEVQAGSHFVSQTGSLRLRQLF"
     gene            12206..13168
                     /locus_tag="VHV99_06025"
     CDS             12206..13168
                     /locus_tag="VHV99_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX3635036.1"
                     /translation="MKPLLTPGTSRISQQDLNELMTLLEIQVLGLSECLVSPGFILEL
                     DGNTAPGIHYILSGAGRLHIEGAGPVELKPHTLLIVPPRHPLRLEVPGHHAGIAPTTI
                     LGREHKQVVDSVNRFRAGNEEPMTVMICGYFSATYGASINLFQTLSTPIVEHFEAADR
                     LDVYLRTAFDELAAQEVGAGAMSGALLKQVIVALVRRSLKSNALWMERFALLGDPQIA
                     RAFADMVARPAAPHTVASLAQSAFLSRSAFMARFSEALGQSAMQVLRDIRMRQAAAQL
                     RTGDVTLDQVARNAGYASRGSFVRAFRDVYGTGPAEYRIDPRAT"
     gene            13259..13759
                     /locus_tag="VHV99_06030"
     CDS             13259..13759
                     /locus_tag="VHV99_06030"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013689737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX3635037.1"
                     /translation="MSYEYKVVASPVGQLKLVASHAKLVAILWENDRPNRVKLGQMIE
                     ADDNPVLMETARQLGEYFAGTRTRFELDTEFVGTEFQKKVWNALLTIPFGETRSYREI
                     ATQVGNSRATRAVGAASGRNPLSIVAPCHRVIGASGDLTGFAGGLPRKAALMAIEGNT
                     TPRRRA"
     gene            14041..14583
                     /locus_tag="VHV99_06035"
     CDS             14041..14583
                     /locus_tag="VHV99_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070849.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HEX3635038.1"
                     /translation="MSRIAVPTPEQTPDASQPVLDAVTKGLKMTPNLFRVMALSPNAL
                     NGWAALQSALAKTLDVKTRDGIALAVSQVNGCQYCLSAHTYVASNIAKIPADEIRRNR
                     LGQADDPRTAAAVAFARTLMTTRGKVSDADLAMVRAAGFSDANIVEIIALSAQYMLTN
                     FVNNVFDTEIDFPVVDLEVK"
     gene            14628..15452
                     /locus_tag="VHV99_06040"
     CDS             14628..15452
                     /locus_tag="VHV99_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEX3635039.1"
                     /translation="MTRTVAEKRAAFRALHTSGCFVLPNPWDAGSARYLQTLGFQAVA
                     TTSSGFAWSVAHADNSLPRDAILAHLRDIVEATDLPVNADFENGFGEDPAGVAESVRL
                     AVATGVAGLSIEDSTGDARASLFPIEVAVERLSAARRAIDENGGDTLLIGRAENFFAG
                     LPDLDDTIARLKAYAAAGADCLYAPGIQTREQIEAVVAAVAPKPVNLLIGSTSTLTLQ
                     DVAALGVRRISVGGALARTAWGGFMQAAQALADGRFDGFAGAASGAQLNDIFKDRA"
BASE COUNT         2798 a         4817 c         5010 g         2993 t
ORIGIN      
        1 cagtatctcg gcggaaaggg tttttgtggc gcgcaggcga tcacgcgttc caggtgaacg
       61 agttcgctct cgaaaatgtg gtgatgcatt gttgatcccc gtggatggcc gtgtggtccg
      121 ttcgcgacgt cactaacaat ctgacatcgc gtagggttgc tcaagcaggt tctcttccgc
      181 gcagtggata tccgcggcga tgcaatgcaa aaaaaccgct tacctggcta tccagagaaa
      241 ccgccggggt ggccggggtg gacgagggcc gcagtcagac tatttgaatg ctctgcggac
      301 gatcggtaga ttgactgcgg ttggcgcatt gccaattaaa acgggcgggc cgccactgct
      361 ggttcccgga aggaaggcaa cggcggccga acctcgcagc ggcacgtctg gtgcgtagcc
      421 agaagaattc cgctctatcg gcgtcaagca tcgcaacgat cttctctatg gaagcattcg
      481 gtcgccatct gctgacgcat gacagtcaca actacaacaa cagcacagcc ctctctcagt
      541 cggttgccaa cgattcggtt tcgtttgcga tgatcggcgc gtcggcaatc gttcggatgg
      601 ctaatcgaac aatcgcgaca gctcgtggcg atccttcggt ttagcggcgt cggctatcca
      661 gaaacaaacg ggacgaggca tgacgtcggt gcgtcgaaca cattgcagcg cgcgatcttt
      721 atttggtctc tcagcttccc cggtgttcga aaagttcttt gcacgctctg gccacggcta
      781 ccgccggtcc gtgttgtctt gttcaggcgg tggcgttatc gaatttgtat aagcaatgac
      841 catgccaaat tgggatagcc ccatgggcgc gggaacatcg gccaatttca tgcgcgacgt
      901 tggacaatct cgtccatgca ttccgcttaa attggacgag ttcgaccaaa tgtcccataa
      961 cggcagcggg atgggccatt tcgtccgcat ggggggaagt cgatctggcg cggcaacgca
     1021 aactcacaat tacagcggtc ccggatcttc ccggcgcaat tcataggcca tttacgattc
     1081 cattactgtc gaggtcgaaa gaagcgtccc ggagttcggc gagcaaaccc atggcgcggg
     1141 tgaagcgagt ggtcgggtct gccgtcaggg aacgcagccc acgctcaagc gtgtcgattg
     1201 cttcggcgat gcggccggtg gagcgcagca ggtcggcgag gtaacgcgcg gcgtccacgc
     1261 gcgccggcac cgagccgaac gctagagcgg tggacagcgc ggtacgcaga cccagttccg
     1321 cggcgttcac ttcgccgaga gcccacttcg cgcgtgcccg ggtgacgagg agttcgccca
     1381 ggtagacacg ttcctggcgg gcgagcgtct cgtcgatcgc ggcatcgaga atcgcctcgc
     1441 aggccttcgc gcggccggcg cgcagcagca gctcgccttg ttgccacgcc ttgaaggaat
     1501 agaacagcgc gccgccattg caaacgacgt ggttatcgaa accgtcgagc atgacggctt
     1561 ccgcttcgtc ggaatggccg cgcgaactga ggtggcacgc gcggaggatc tggcccacgc
     1621 cccgatagaa cgcgaagccg ttcgcgatcg acaccgattc gagttcgctc gcgaggtgcc
     1681 cgagccggtc cacgtcttcg aacaggcagg cgagccacgc agcgccttgc aaattgacgg
     1741 catgagcgaa cgcgtgcgcg cccggctcgg acgcgcgcag gatcaacatc tccatggcgc
     1801 gccgcgcttg cgcgaatgcg ccgatctggt aggcggcgag tccttcgaaa gtcaaggcga
     1861 gactggcgag gtcgatccct tgcgagccgg tacgggtgtc ctcgcgtgct acgccaagca
     1921 ggttgccgcg cgcgagacag gcgagggctt cctcgctgtc gccgagccag aagagggtgt
     1981 tggtcatcgc aacgtgcgct tcgtcgagcg aaacccggtc gccgccgttc tgcgcgcgct
     2041 ccagcatgtt ctgggcggtc gcgcgagcct gcttgagttg cagtgtcgtc agttgggtgg
     2101 tccagatgcc gaattgaatc gcggcgattt cggccggcgc acacacgcct tcgccgacgg
     2161 ccagcgcagc ggcgtagcag gcggtggctt ccgcgtacag ccagccgtgc acgctgcgca
     2221 agctcatgcc gagcaggcgg taggcgtcga actgaagacg gcgcgcggtc tcctgttcgt
     2281 ggcacgtctg cagcagttcg agacagcggc gcaactgcgg aatggcttga gcgaacttcg
     2341 actcctcgat cagcgcgttc gcatggtcga gacacttgcg cgcttcctgg tgatgttcat
     2401 cgcgcgactt cagacgccgc tcgatggtct tgcggcccac gcccagcagt gtcgccgccg
     2461 cgctcttgtt gcgcccggtt cgctcgagcg cgcggtcgat caggaggtct tcggcggcgg
     2521 cgagcttgtc gcgtccttcg agttgcaaca acatatcggc gaggcttgcg cgcgatatgg
     2581 gcgcgccggt ttcgttggcg agaaacggtt cgagcgagtc gacgtcgatg agcgtgtttt
     2641 cggcgagcac gctcaactgg ctgatcaggt tgcgcaattg ccggatgtga cccggccatg
     2701 catgctggct cagccggcgc agggcagccg gtgaaaattc gagccgacgc gcatgctgcg
     2761 acgcgaagtg gttagcaagg gcagggatgt cctcgatgcg ctgctcgagt ccggggactg
     2821 caagcacgaa gaccgcgagc cggtagaaga gatcctcgcg aaatagccct tcgcgcgcga
     2881 gttcgcgcag gtcgcggtgg gtcgcggcaa ctacgcggcc ttcgaagtgc agactggttg
     2941 acgaaccaat cggacggaac gtgcgcgttt caagtacgcg cagcagtttc ggttgcagca
     3001 cgagcggcag ttcgccgatt tcatcgagca gcagcgtgcc cttgccgacc tgctggaaaa
     3061 gtccctggcg gttttcgccc gcgccggtga atgcgccttt gacgtggccg aacaattcgg
     3121 cttcgacgag gttttctgga atcgcaccgc aattgacgtc gacgaacggt tggtcgtggc
     3181 gcaggctgtg cgcgtgcaca cggcgcgcga ccagttcctt gcccgaaccg gtcggcccaa
     3241 aaatgagcaa cgggtgatcg gtaggcgcga cccgatcgat catgcgaacc agttgttgaa
     3301 acgcgggcgc gccgcccaca aaggccccgg aatcagcctt gtcgtgggta aatggaacga
     3361 caggggtata actcattaca aagtacgctc cacatgatcc gcgcgtggcg ccacgcggaa
     3421 gttgccgtcg aaagacgacg gtcatgcatt gatctggggt ctggcgaagt cttccgattg
     3481 cgctgccgga accgtccgcc gctagaagag acagagtatg acggctcgtt accgctattg
     3541 tcttttcgaa attaattttc gcgccaaggg catgtgcgga ttctcgtcgc aatgtcttgc
     3601 aaacaaggta gcgatttcaa ccttgggcgc tgtcagcagc atcgcgatcg attggcccac
     3661 tgcgtcggag cgcacgccgc gtgactgacg gttggcgatc aaccggacat accgtcacat
     3721 gctgccgttt tccaccacgc ggccttcggc acgccagcgg agcatgccgc cggcaaggtt
     3781 ggcgacccgc tcgaagccgg cctggcgcag catgatcgtg gcttgtgccg agcgtccgcc
     3841 ggcccggcag acggtgacga tcgggcgctc cttcgtgagt tcggcggcac gcccggccag
     3901 ttcgcccaat gacatgagcg tggcggcggg gatgcgcccc agcggtccgt tgaattcgtc
     3961 gggctcgcgc acgtcgacga tctcgaccgc gcgcagattt tcctcgagcc actggggatt
     4021 gatttcccag atgcccgcaa agctgcaggt gagtggcgcc cgatcgggct cggccatccg
     4081 cgcggggtaa ctcgccgccc agccgcattt cagattcgcg ggcaccgcga cgtcgatctg
     4141 tttcggatgc ggcaggtgca ggttcctcat gtatccggtg aaatcgcttt cacacagttc
     4201 accgccaagc cgtggattga agcgccgctc ctcgtcgaca ctggtcaccg tcaaaccgcg
     4261 atagtcgtgg gccgggtaga gcaggcacgc ggcgggcagc gtgaagatgt ggttgtggac
     4321 cgcgcgaaac atggcgtgtg cgtcaccgcg ctgaaaatcc gtgcggccag tgcctctgat
     4381 caacaggcaa tcgccggtaa acgccatgct ctcgttgtcg agtacgagtg tgatgcagcc
     4441 atcggtatga cccggtgtcg cgcgtattgc caggtacctc gcgccgaact cgaccttgtc
     4501 tccctcgctc agatagcggt ccgcgccttc ggcgccgctc gcagccgata tcgcaatccg
     4561 gctgcctgtg cggcgattca gcatccatgc gccggtcacg tgatcggcgt ggacgtgggt
     4621 gtcgatcgtg taaagcagat gcaggccaag ctcttcgatc agggcggcat cccggcgcac
     4681 ctgttcgaaa accggatcga ccagcaccgc ctctcgcgtc gtgctgtcgg cgagaagata
     4741 cgtgtaagtt gacgattgct gatcgaatag ctgccggaaa atcaacacga tttttgctcc
     4801 gcggcccgct cctgcgaggg ctttaacttt acttgggttg ctcaacgtag cgcaagtagg
     4861 gcttgacggt cttgaaaccc tgcggatatt tttgcatcgc ggcatcgttg gaaacggagg
     4921 ttggaatgat cacgtcttcg ccgggtttcc agtttaccgg tgtcgcgaca gcgtgtttcg
     4981 cgttgagctg cagtgcatcg agcagccgca tcacctcatc gaaattgcgg cccgcactca
     5041 ttggatagac gagcatggct ttgaccttct tgtccggccc gatgagaaac accgaacgca
     5101 cagtcgcgtt gtccaccgcg gtgcgcgggc cgccgccgct cgcctcagga tgaatcatgt
     5161 cgtagagctt cgcgaccgtc aggtccgggt cgccgatcag cggatagccg acctcgtggc
     5221 cctgggtctc cgcgatatcc ttcacccact cctggtgatc actgacggga tcgatgctga
     5281 gtccgataat tttcgtgttg cgcttatcga attctggttt gaggccagcc aggtagccga
     5341 gttcggtggt gcaaacaggg gtgaagtctt tcgggtgcga aaacagaacc gcccaatggt
     5401 cgccgatcca ttcgtgaaaa cggatcgtgc cttcggtggt ttcggcagtg aaatcgggag
     5461 cgtcttctcc tagtcgaatc gacatcatca gtctccatag ttagcgtgca tcaaggtagc
     5521 agtgcctcgg ccggagtgaa gtcctccggg cccggctgtt tggtcaggcg attggcgtga
     5581 tccgggatca ccctcctgcg gactgcacgg tatcgcgcca aaggaccgtc cgcgggcgca
     5641 gcagattcag agacttccca tccagccgtg atgcgggaca gacatcggga cacgctgcgg
     5701 gccgcccata tagggtagaa caaaaaagac gctggccgta ggcaaaccgt agggcgaaac
     5761 cgccgttgtg acgattgcct tgccagccga catcgcgaca ggaatgccgc caacagctca
     5821 cgcctggaca ggggcccagg cgtcgtctga cgcagttcct gcaaacgcca atcaggccgt
     5881 gatgaacgcc agcagatcgg cgttgatctg ctcggcatgg gtggttggca tgccgtgcgg
     5941 caatcccggg taggatttaa gcgtcccgtg cttcaaaagc ttgacggatt gggctccggt
     6001 ggtagcgaaa gggcagatct gatcgtcctc gccgtgcatc accaggacag gcacatcgat
     6061 gatcgccaga tcgtcgtaga actcggtttc cgacaggacc tggatgcagt catattgggc
     6121 tttgatgccg cccatcatgc cctgccgcca ccagttctcc tggatgcctt cggagatcgt
     6181 cgcaccagga cggttgaaac cgtagaacgg aatcgtgatg tccttgtaaa actgggaacg
     6241 atggttcgcc gtgccgttgc ggatctggtc gacaacttcc cttggaacgc cctcggggtt
     6301 cttttccgac ttgatgaaca gtggcggaat cgcgctgatg agtaccgcct tcgcgacgcg
     6361 tccgttgccg gtgccgtgcc gggcaacata gcgcgtgacc tcaccgccgc ccgtggagtg
     6421 accgatgtgc accgcgtcct tgagatcgag cgccttgacg agttcggcaa cgtcggcggc
     6481 gtaggtgtcc atatcgttgc cgctcacagt ctgggtcgat cgaccatggc cgcggcgatc
     6541 gtgagcaatc acgcgatatc cgtgcgcgag gaaaaacatc atctgggcgt cccaatcgtc
     6601 ggcggaaaga ggccagccat gatgaaagac caggggttgg cccgtgcccc agtccttgta
     6661 gaaaatctcg gttccatccg cggtcgtgat ataggaactt cccgtgttcg ctttcatttc
     6721 gaatcctttt ctgaggtgga tgagatgaag ccggccgcag gtgccgggat ttctgtcgaa
     6781 atccggcctg cgaccgtcag cgtattcggg gccgcccaac accacaacgg acaaatctga
     6841 caaaaggcat ggaaaaaacg acagcggcct caaagccggc gatttcgcgt cgtgttgctg
     6901 tttcatttcc cggacgcggt gtcagtgaat ggtctttagt tgcgaccaca accggttctc
     6961 gaaacgcgga attgcgatcg gcttccgctt caacgtgtcg tctcttccgg gcatgtcggc
     7021 acgtcgtgca gcagcattgg ccaaaggcga gcccaatcgc cctcgtgcgc aacgttcgcc
     7081 acgctgcgcg tcgctgcaca gtggccaccg gccgcggcga tgaagcgctg tccgatcgcc
     7141 ggtggaacga cggtgtcggc gctgccgctg aaatggatct ggggaatggt ggcgacgcgc
     7201 gtggccacgt cgatgggatt gccggaatcc ggcatcggcg aaacgtcatg cagacggttg
     7261 acgaaggctg tgtcgagatt gccggccacc gtgcggatcg agccgatatc ggaacggcgc
     7321 gcggcgatca ataccgcaag cgcgccgccg cccgaataac cgacgaggtc gatgcgctga
     7381 cccggcacgc gggcggcaaa gcggtcgagc gcctgatcga gcgagtgcac gacttccggc
     7441 gcgaagcgct tgccggtcca atagggaatc ccgcagtcgg gattcgccgc catgggcgtg
     7501 aactggcaag gacgtgcgag ataaacgacg ttcggttccg ggtcggcggc ggcgagggct
     7561 aagcccgttg cctgatgggg agtagggtcc agcgatggct cactgcgcga tatccaggcg
     7621 agcccgtcgc cttcaatgta gaccgcgagg gggcggtcag ggcgcgtgat gcgagtgaag
     7681 gcggtcagca cgaagcggtc ggtgcggacc gcttcgcgct ttagaccggc agcgttcgcc
     7741 agcgcatcgg cgtgcgcgtt cggatcgagc gcggcgcaac cggcgagcca caacgcaaga
     7801 caaacggccg ccggccactg ccatcggaag gtacggcagc gcggcgcccg tggtggtgtc
     7861 gccgcgttca ctgcgttcgc gttcaccgcg cttaccccgc tccaactcac tgcactcagc
     7921 acacccacgc tcgccgcatt caccgcgctc acgtttgccg cgcgcccgcg cgaagtagcc
     7981 aaagcgcgtc ggcgcggcaa cgtgagcacc tgccgcctag aacagctgac gcaaacgcaa
     8041 cgagccggtc tgcgaaacga agtgcgaacc ggcctgcacc tcataaagcg cactgagact
     8101 cagattgctc gcgcgcagca gcgtcacgcc cagctgaatg tccgcgagat cggccaccgg
     8161 cgttgcgccc actgtcgtga acgcggtgct gccggtcgga tcggcggcat agctcgcgcc
     8221 ggtcgcgagg cgcgtgtggt cgtactcgtg cgtccattgc atctgcagat acggaatgat
     8281 gtcgccgtat tggctcgcga agcctttctc cagcttcagg cccgcgttgc tgcgcaccga
     8341 agtcgcgtgc gtggcgccga cggacagcgc cgcgccgttg ccgccgcttt cggtgtacga
     8401 gccttgatgc agatagctgt aagcgaggct cgcgagcggc gtgagcgtca ctgcgccgaa
     8461 cgcgagcgga tagccgaact cggcgctcgc cacgtactgc tgcccgttga actggccgtt
     8521 cgcgacgccc gagaagccgg tgaaatcgac cacgcgggtc gagtcgtagc gctgctgcac
     8581 ggcgcccgcc gcgaggttca cataccaggg cttgccgctg tagctggcgt agccgatcag
     8641 gccataggcg ttcacgcgcg tcgaatcacc tgcgctgttg cccgtgttgt tgaccgcggt
     8701 gttcgtgtag ctgaatacgc cgcccgcgcg ccagttgtcg ctcaccgaac ggtcgacacc
     8761 gaccagcagg ccgccatagt tcgcgctgta gccgtccacg ccgtcgagct cgccctggcc
     8821 cgcatggccg ccgaacgcct gaccccacac gcccgacgcg ggcgctgctt cacctgtcga
     8881 gatcccggtt gcgccgttgg cttgtgcgag tcgcaggctg tcggcgtgag cggagacgac
     8941 gttcagcgcg tccaacgtgg gcgcggctgc cgcgcgactg agcgacgctt gcgccgtcgg
     9001 gctcagttgc gtgcccgcgc gcgtggccgc ggcactcgag ccgaggctga gcgccgtcgc
     9061 ggcgttgtac aggttgagca gcgcggggct gacgcccgaa tatttcgcga ttcccgacag
     9121 cgccgcgttg gcattcggcg tggcggccaa tgaactggcc ggctggccgg cgagattgac
     9181 gaccagctcg ctgcggccgt tcgcgttcac gtccgtgccg aacagcaagc cgctgtagcc
     9241 cagcgccgag tagtcgagcg tatcggtgtt gtagttcgtg ccgttagccg atgcatcgac
     9301 gaccacgaaa cgctggcccg gcgcgaacgc atagctgtcg ctcttcacga gttggaccga
     9361 cgacccggcc gccagtgtcg cgccgccggt gacgacgaga cggccatagc cgctgtcgct
     9421 gctttcgttg ccggtcgcca cggcgccgtt gcctaccgtc acgcgcaacg ccgcgtccgc
     9481 gctttgcgtg tagttgccgg tgacgctcag ggcgccgccg ctgcccggtt gcatcacgcc
     9541 gctattgtcg acgttgccct tgatcgtgcc gtaaccggca agcgtgccgc ccgaggcgac
     9601 ctgaacgctg ctcgcaagcg aggctgtcga tccggcgttg ctgccgacct gcaacgtgcc
     9661 ggcctgcacc gtgctcgcgc ccgtaaaggt gttcgcgccg ttcagcacga gcgtacccgt
     9721 gccggtcttg gcgatgccga agctgccacc cgattcgccg atcgcgccgc tttgcgaggc
     9781 agagttcgaa ccgtcgacct cgagcgtcgt gttgctgccg agggtcactg cattggccag
     9841 attgataccg ccgagatacg caatcgtcga gttggccgta ttcagcatgc cggtgccgag
     9901 cgcgcggctg ttgcccagta ccagcatgcc gtcgctcagc gtcgtcgcgc cgctatacgt
     9961 gttcgcgccg aacagcgtga ccgtgccggt accgatcgaa acgctcgcga agttcgagat
    10021 cgtgccgccg tacgtgaagc tgttgcccgt gccgggcacg atcgtcagcg tgctcgtgcc
    10081 ggtgccgccc gaaatgccgc cgttgatgac cgcggtgccg ccgagaatct gcaggctgct
    10141 gttgccgctg ccgaggtcga ccgcgcggcc gctgctgtcg gcggtgatgg tgccgtagtc
    10201 gatcacggtg ctgttttgcg cgccggtgtt gatggccgcc gcggtcgcac cgccgccttc
    10261 gatcacgccg cccgcgaggt tgacgatcgt gagcgagtag gcggtggccg cgccggtcgc
    10321 cgcgatgccg gaatcggtgt cgcccttgat gagcccgctg ttggtcacgg tggtgttgcc
    10381 gtagatgccc tggatgggaa tcggggcgtc ggtggtcggg tccttgtcga tgccggccag
    10441 cgtgataccg cggccgatgc ccgtgttggc cgtgccgtcg gcattggttg cggaattgct
    10501 gccctcgatc aggcctgagt tgacaatagt gccgccgccg accgtcacgc cttcgctcgc
    10561 gtcgttgtac gcatgcagcg atttgatcgt gccggtattc gtgaggttga cgagcccgtc
    10621 gacatccacc ccgtcgccat cgccggtcac gccgttgccg gtgatcgtgc cgttgttgac
    10681 cacagtcacc acttccttgc cgttgaagcc gtcgatgttg ataccggaac cgttgtcgcc
    10741 ctggatcgtg ccgccgcggt tgttcgtgac gctcagcgtg aacgtgccgt cggtggtgac
    10801 gtccgtgttg ccgccggtaa tgccgtgccg cgcgccttcg atgaggcccg tgccggccga
    10861 cgtggccgtt ccggtcgtgg cgttgacgat cgtcacgccg ctgttgctct gcaagtccac
    10921 accgtcgctc gaatcggtgc tgcccggatt gttggtcgag cggatgatgc cgtcgttgta
    10981 gacgaagccg ttgacgcccg gacgcacggc atccgcttcg ttggcctgaa tcacgccggt
    11041 gccgaagttg tacagcgtgt tgctgccggt ggtgatcgcg ttgaagtcga tggcctgcga
    11101 gccgccggcc gacgcgttca gcgacgtcag cgtccccgag ttgtagaacg tgacgttgct
    11161 gttcggctcg ttcatctgga tgacgtcggc gtccgcggtc tggatcgtcg cgcccacgcc
    11221 attcgtgacg gtcaggctca ggccgccggt gttgtcgcga atcgcgcggc cgctgccggt
    11281 ttgcgtgatc gtgccgttgt tgacgatgtt cgcattgccg gtgacggtaa tggccaccgt
    11341 gctgcccgat atcgacaggc tgccgccggc gtcgacggtg ccgttcgcgc cgcctgtgcc
    11401 gagggtttgg gccgtggtgc tggtcgtgcc gttcggcacc tcgaacgacg cggggccggg
    11461 tttagcccag acatggcttt gctcgaagac tgccagtgcc ccgagcacgg caaggatgat
    11521 ctgcttcttt tgcatgagag gtcgtagcgt gtagttaagg ttcttgtttt acgaactaag
    11581 gttcttgttt tacgaacgcg cgcgcaaccc ggcccggtcg gcggggcgat gctggcatct
    11641 ggttcagcgc gggaagcgtt gacggaagca gcaggggttc gctgcgtgcc ccgctatgcc
    11701 gctgatgagc ggctgaagga cgcgtgattg tgtgacgggt ttgtggcgtt atgatgacgg
    11761 ccatacagcg cttgttgggt gtgggcctac ggggttggcc tttccttgat ctgacttttt
    11821 ggcctttcct tgaattggta ttggtctatt agtgccgccc cgcacagggg caacgctaat
    11881 agaccactaa gaactcaagg aaaggccaac accgaaaggc cacagacaaa ataaacactg
    11941 ggcgagcaga gaaaacaaga aaggcccaac gccgcaggca aacagacaga caaacaaacg
    12001 ctacaaaagc aaaaaaactt caaccgtgcc ggaggcaata ctcagcaaca caatcgacga
    12061 ggcgcatcag gccgctatgg aaattgacgg aaggggtgag aaaatgcctc aatagtcgat
    12121 ctttggccca tcgagcgttc tatcgcgtgg atatgggcgc gattcagata gcgcaggctt
    12181 gcccggcgcg cgatcctatc gaggtatgaa acccttgctc acacccggca cctcccgcat
    12241 ctcgcaacag gatctcaacg agctgatgac gcttctcgag attcaggtcc tcggcctgtc
    12301 tgaatgcctg gtcagccccg ggttcattct cgaactggac ggcaatacgg caccgggcat
    12361 ccactacatc ctctccggcg cgggcaggct ccatatcgaa ggagccggcc cggtggagct
    12421 aaagccacat acgctgctga tcgtgccacc gcgtcatccg ctcaggctcg aagtgcctgg
    12481 ccatcatgcg ggaatcgcgc cgaccaccat cctcgggcgc gagcacaaac aggtcgtcga
    12541 ttccgtcaac cgcttccgtg ccggcaacga ggagccgatg accgtgatga tctgcggcta
    12601 cttcagcgcc acctatggcg cgtcgatcaa tctgttccag acgctctcga caccgatcgt
    12661 cgagcatttc gaggcggcag accggctcga cgtgtatctg cgtacggcgt tcgacgaact
    12721 ggccgcgcag gaagtcgggg cgggggcgat gtcgggtgcg ctgctgaaac aggtcatcgt
    12781 cgcgctggtg cgccgctcgc tgaagtccaa tgcgctctgg atggagcgct ttgcgctgct
    12841 cggcgatccg cagatcgcac gggcctttgc cgatatggtg gcgcggcccg ccgcgccgca
    12901 tactgtcgca agcctggcgc agagcgcgtt tctgagccgc tcggccttca tggcgcgatt
    12961 ttcggaggcg ctcggccagt cggcgatgca ggtgttgcgt gacattcgca tgcggcaggc
    13021 cgcggcccag ttgcgcaccg gcgacgtcac gctggaccag gtggcccgca acgccgggta
    13081 tgcgagccgc ggttcgtttg tgcgggcctt ccgcgatgtc tacggcaccg gtcccgccga
    13141 ataccgcatc gacccgcgcg cgacctgatt cccggacgat ctgcgctgtc tcgcggacct
    13201 tctgcaaagc catcccgcct attcttcctt taccgtattc aacgcaagga gagacaggat
    13261 gtcttacgaa tacaaagtgg ttgcgtcgcc ggtgggtcaa ctcaaacttg tcgcgtcgca
    13321 cgccaaactg gttgcgattc tctgggaaaa cgaccggccg aatcgagtga aactcggcca
    13381 gatgatcgag gccgacgaca accccgtgct catggaaacc gcgcgtcagt tgggcgagta
    13441 cttcgcgggc acgcgcacgc gcttcgaact ggacacggag ttcgtcggca ctgaattcca
    13501 gaagaaggtc tggaatgcgt tgcttaccat tccctttggt gaaacccgca gctaccgcga
    13561 gatcgcgacc caggtcggca atagccgtgc tacccgggcg gtgggggcgg cgagtggacg
    13621 caatccgtta tccatcgtcg caccttgcca tcgcgtgatc ggcgcttcag gcgacttgac
    13681 cggctttgcg ggaggattgc caaggaaggc cgcgctgatg gcgattgaag gcaacacaac
    13741 tccgcgacgt cgcgcataag ctcgatttcg ttttcgaacg cgctttccag cggcgccttg
    13801 tatcgacgtg attcatgtcg gcacaaggcg ccgattttta ttggcgtcgc gaatccgcgg
    13861 ttggcgcgcg gctggaccgg cggtggactt tcgcgatcgt tttcaggacc ccgtgcaaag
    13921 gtgccggacc tagacttcac agcaatgcag aaacgcaaac gcttcatgca gacggtccca
    13981 agtccatccg ctaaccgatc aacgatcgac gatcgacgat caacgatcag gagaaccacc
    14041 atgtcccgca ttgccgttcc gacacccgag cagacccccg acgcatcgca gccggttctc
    14101 gacgccgtca cgaagggtct taagatgact ccgaacctgt tccgcgtcat ggcgctgagc
    14161 ccgaacgcgc tgaacggctg ggcggccctg caaagtgcat tggcgaagac gctcgatgtg
    14221 aagacccgcg acggtatcgc gctcgctgtc tcgcaggtga acggttgcca gtactgcctg
    14281 tcggcccaca cgtacgtggc cagcaacatc gcgaagatcc cggccgatga aatccgccgc
    14341 aaccggctgg ggcaggccga cgatccgcga accgccgcgg ccgtggcatt tgccaggaca
    14401 ttgatgacaa cccgtggcaa agtatccgac gccgatctgg caatggtgcg cgccgcggga
    14461 ttcagcgatg cgaacatcgt cgaaatcatc gcattgagcg cacagtacat gctgacgaac
    14521 ttcgtcaaca atgtgttcga tacggaaatc gacttcccgg tcgtggacct cgaagtcaaa
    14581 tgacccgcac gaggagcttc tgctcctcat ctttcaggag acacgtcatg acccgcaccg
    14641 tcgccgagaa gcgcgccgcc tttcgcgcat tgcacacatc cggttgtttc gtcctgccca
    14701 atccgtggga tgccggcagc gcccgttatc tgcagacact cggcttccag gccgtggcta
    14761 cgaccagctc cggcttcgcc tggtccgtcg cacacgctga caattcattg ccgcgcgatg
    14821 ccatcctcgc gcatctgcgc gatatcgtcg aggcaaccga cctgccggtc aacgcagatt
    14881 tcgagaacgg cttcggagag gatcccgccg gcgtggctga aagcgtcagg ctggccgtcg
    14941 caaccggcgt tgccggcctg tcgatcgaag attccaccgg cgacgccagg gcctcgcttt
    15001 tcccgatcga ggtcgccgtc gaacggctca gcgcggcacg ccgcgcgatc gacgaaaacg
    15061 gcggcgatac gttgctgatc ggccgcgcgg agaacttctt cgccggcttg cccgatctcg
    15121 acgacaccat cgcgcgcctc aaggcctatg ccgcggctgg cgccgactgc ctctatgcgc
    15181 caggcattca gacgcgtgag cagatcgaag ccgtggtcgc cgccgtggcg cccaagccgg
    15241 tcaacctgct gatcggttcg acttcgacgc tcacgctgca agacgtggcc gcgctcggcg
    15301 tgcggcgaat cagtgtgggc ggcgcactgg cccggacagc gtggggtggg ttcatgcagg
    15361 ctgcgcaggc gctggccgat ggacgcttcg acggctttgc cggggcagcc tccggcgcgc
    15421 aactcaatga cattttcaag gaccgtgcat gaggcccggc gtgatgtccc agatgacacc
    15481 cgacaggtgg cctcgcatga ggcccaacat gtggcctgac atgacactcg acatgtggcc
    15541 cgacatgacg cccgacataa cacccaacat gaggcccagg catcgggccg cgcatgcccc
    15601 ttcacatgga gatgagca
//
LOCUS       DATASH010000214         4918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2204689,
            whole genome shotgun sequence.
ACCESSION   DATASH010000214 DATASH010000000
VERSION     DATASH010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4918
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2204689"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            394..645
                     /locus_tag="VHV99_06045"
     CDS             394..645
                     /locus_tag="VHV99_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HEX3635040.1"
                     /translation="MSLNFPNPSRSYDPYRHCVNFWGYDNAREIAFTVNNSVISNLSP
                     SAGSDEPAVLAAFDRHREQILTLARGLYVGGPQNRYTIS"
     gene            763..1026
                     /locus_tag="VHV99_06050"
     CDS             763..1026
                     /locus_tag="VHV99_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635041.1"
                     /translation="MDDDQVNLEEIADSKRRREAARLAEIDGSTAHAVPTAPTAITPQ
                     PVQPGGEAAPQQWVKHVTKWLGAKGTLMQTRRALTLRKKPAAL"
     gene            1245..1961
                     /locus_tag="VHV99_06055"
     CDS             1245..1961
                     /locus_tag="VHV99_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007738321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX3635042.1"
                     /translation="MDKGDPKTNHLLAVLPSAEWARIEPYLVEVELPLGEVIYESGGQ
                     LEHVYFPTSSIISLLYVMEDGASAEIAIVGHEGIVGVSLFMGGETTPSRAIVQSAGRA
                     WRLNARMLKDEFNRAGPMQHLLLRYTQALITQMAQTAVCNRHHSIDQQLCRWLLLSID
                     RLPSNELRMTQELIANMLGVRRSGVTAAALKLQNAGLIRYSHGHITVIDRPGLETHVC
                     ECYAVVKREFDRLLPALQAI"
     gene            1977..2576
                     /locus_tag="VHV99_06060"
     CDS             1977..2576
                     /locus_tag="VHV99_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635043.1"
                     /translation="MCVQGEPLFGSLQARYIAIAIAIAIAIAIAIAIAIAIAIAIAGA
                     ITGAITPANTAPSTCRCRTHGVRRRLTFDLFEYVHQPADALQSARRQACFDGRQGENP
                     TSPIGQAQRRGSDEKRNNLFEMSELALEWVVRHGVPVRAVIAFTQHARHARPCLHATV
                     QDAKSRPTPINAWLPPDCSQGIASVGPRLKQPFRVSSLQ"
     gene            complement(2542..3471)
                     /locus_tag="VHV99_06065"
     CDS             complement(2542..3471)
                     /locus_tag="VHV99_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3635044.1"
                     /translation="MPFDERMLNGMGVLTAIVDCGSFAAAGEALDMSQSGVSRSVARL
                     EARLGIRLFDRTTRSVTLTDEGRRFYEQIVPLLGGLEEAAASAAQGATAVRGRLRVNM
                     DPFFSRLVLGPRLGGFVDKHADLQLELITRDQLGDMVADGFDLAIRFGDPPVSTLIAR
                     KLLDTRIMTVAAPSYLKKHGHPASPAELESGKHVCIKFRDPLTGYPFSWEFHRGRKKM
                     VITPRGRLTVNDVGTLHSVCAAGQGVAQILALGAESLLANGKLVELFPDWGDEVYPLY
                     ALYPSRHHPPAKVRAFFDFIVSLTGGSTRETVV"
     gene            3723..4496
                     /locus_tag="VHV99_06070"
     CDS             3723..4496
                     /locus_tag="VHV99_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3635045.1"
                     /translation="MSRVFITGSADGLGQMAARLLVAAGHQVVLHARSAARAEQALKA
                     VPAAETALIGDLSSIAATVALAEQVNTLGRFDAVIHNAAVGYQEPRRIATLDGLPHVF
                     AVNTLAPYVLTALIRKPKRLVYLSSGLHRSGDASLDDLAWERRPWQGTAAYSDSKLHD
                     ALLALAMARRWPEVLSNALEPGWVATRMGGAGAPDDLQAAPETQVWLAVSDEPAATVS
                     GAYFYHLKPRETHPAVHDVAVQERLIEACEGFSGVRLPD"
     gene            complement(4600..>4918)
                     /locus_tag="VHV99_06075"
     CDS             complement(4600..>4918)
                     /locus_tag="VHV99_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3635046.1"
                     /translation="FGAANSMQFAAMNSVTLKGLSHEDAGSGNSLFSMVQMLAIGLGV
                     SIGGGLVNLFSAQWGSAALGFRLSFVCVGVITLVSAWIFRHLDEAPVARGVRGQAAQG
                     AGR"
BASE COUNT         1054 a         1555 c         1439 g          870 t
ORIGIN      
        1 cccgctgaag aatttgcggt ttgagctgct ttctcttgcc tacttctctt tgcagccggc
       61 aaagagaagt aggtgccgcc ccgcacaggg gcaacgctaa tagaccacta agaattcaag
      121 gaaaggccaa cgccaaaggc atacagaaaa caagcgccgc gcaggcaaac aaatcaagga
      181 aaggccaacg ccacaggcat acagaaaaca agcaccgcgc aggtaaacaa gtcaaggaaa
      241 ggccaacgca gtagacacac agagaacaaa ccgctacgca gccaacccac ccccgcgcgg
      301 gggcaaaaaa accaccactt acaaacaaac cccttgaccg cgcctcacat tcagcgcacc
      361 gtgaagaaga aaaaagaacc ccgggagccc aacatgtccc tcaacttccc gaaccctagc
      421 cgcagctacg acccctaccg tcattgcgtc aatttctggg gctatgacaa cgcacgcgaa
      481 atcgccttca ctgtcaataa ttcggtcatt tcgaatctaa gcccgagcgc gggctccgac
      541 gaaccggccg tgctggcagc cttcgaccgt catcgcgagc aaatcctcac actcgctcgc
      601 ggcctgtatg tcggcggccc gcaaaaccgc tacacgattt cttgatatag acagcgcggc
      661 gcggcacgtc caaccggacc tgccgtgcgc cgccttgtgc cacagccgaa gcagcgcggc
      721 gcccaccggc gccttgcctt gctttgtcgc gggagtttca gcatggatga tgaccaggtc
      781 aacctggaag aaatcgccga tagcaagcga cgccgtgaag cagcacgcct cgccgaaatc
      841 gacggttcga ccgcacacgc ggtgcccacc gcacccacgg caatcacacc gcagcccgtt
      901 cagccgggcg gcgaagccgc accgcagcaa tgggtcaagc acgtcaccaa gtggctgggc
      961 gcaaagggca cgctgatgca aacacgccgc gccttgacgc tgagaaagaa acccgccgca
     1021 ctttaagcat ctggaacgaa aataaccggc gtaaagccgg tttttctatt ttggccgcgc
     1081 ccattgcaat tgctggtacc ggcaaagcgg gcgcttgacc cgatctcgcg cctcgggagc
     1141 gggctcataa tcatcgtggc gacgaaacgg gtgcttgcgc cttttctcat gatcaatgca
     1201 tcgccgcgcc gcgagcgcgt ccggaaccca gcaaggagcc accgatggac aagggtgacc
     1261 caaaaacgaa tcatctgtta gcggtgttgc cctcggcgga atgggcacgc atcgagccgt
     1321 atctggtcga agtcgaattg ccgctcggcg aggtcatcta cgaatcgggc ggccaactcg
     1381 agcacgtcta tttccccacg tcctcgatta tttcgctgct ctatgtgatg gaagacggcg
     1441 catcggcgga aatcgccatc gtggggcatg agggcatcgt cggcgtgtcg ctattcatgg
     1501 gcggagagac cacgccgagc cgcgcgatcg tgcaaagcgc gggccgcgcc tggcgcctga
     1561 atgcacgcat gctgaaagac gagttcaatc gcgcgggacc gatgcaacac cttctgctgc
     1621 gctacacgca ggcgctcatc acgcaaatgg cgcagaccgc ggtatgcaac cgccatcatt
     1681 cgatcgatca gcaattgtgc cgctggctgc tgctcagtat cgaccggctg ccctcgaatg
     1741 aactacgcat gactcaggag ctcatcgcca atatgctcgg cgtgcgtcgc tccggcgtca
     1801 ccgcggcggc gctgaaactg caaaacgccg gcttgatccg ctatagccac ggccatatca
     1861 ccgtgattga ccggcccggt ctggaaaccc acgtgtgcga atgctacgcg gtggtcaaac
     1921 gcgaattcga ccggctgctg ccggcgttgc aggccatcta gcgagcccgt gggcgaatgt
     1981 gtgtgcaggg ggagccgcta ttcggctcgc ttcaggcacg ctacattgcc attgccattg
     2041 ccattgccat tgccattgcc attgccattg ccattgccat tgccattgcc attgccattg
     2101 ccggtgccat caccggtgct atcacccctg ccaataccgc gccgtcaacg tgccgttgca
     2161 ggactcatgg cgtccggcga cgccttacat tcgatctttt cgaatacgtc catcaacctg
     2221 ccgatgcgct gcaaagtgcc aggcggcaag cgtgcttcga cgggcgacaa ggagagaatc
     2281 cgacgtcgcc aatagggcaa gcccaacggc ggggcagtga tgagaaacgg aataatctgt
     2341 tcgagatgag cgagctcgct ctcgaatggg tagttcgaca tggggttccc gtacgtgctg
     2401 tgatcgcctt cacacagcat gcgcgccacg cgagaccctg tctgcacgct accgtacagg
     2461 atgcgaagtc gcgccccacg ccgatcaacg catggctgcc gcccgattgt tcccagggca
     2521 ttgccagcgt cggaccgcga ctcaaacaac cgtttcgcgt gtcgagcctc cagtgagcga
     2581 aacgataaag tcgaagaatg cccgcacttt cgcgggcgga tgatgtcggg aaggatagag
     2641 cgcatacagc ggataaacct cgtcgcccca gtcgggaaac agttcgacca gtttgccatt
     2701 ggcaagcaac gactcggcgc cgagtgcgag aatctgcgcg acgccctggc ccgcggcgca
     2761 cacgctatgc aaggtgccga cgtcgttcac ggtcaagcgg cctcgcggcg taatcaccat
     2821 cttcttgcgt ccgcgatgaa actcccagct gaacgggtaa cccgtcagcg gatcacggaa
     2881 cttgatgcac acgtgcttgc cgctttcgag ttcggccggg ctcgccggat gcccatgttt
     2941 cttcagatac gacggcgcgg cgacggtcat gatccgcgtg tcgagcagct tgcgtgcaat
     3001 cagcgtcgaa accggtgggt cgccgaagcg gatcgccaga tcgaatccgt cggcgaccat
     3061 atcgcctaac tggtcgcgcg tgatcaattc gagttgcaga tcggcatgtt tatcgacgaa
     3121 gccgccgagc ctcggcccga gcacgagacg cgaaaagaac gggtccatgt tcacgcgcaa
     3181 acgtccacga accgcagttg cgccctgagc cgccgaagcg gccgcttcct cgagtccacc
     3241 aagcagcggc acgatctgtt cataaaagcg tcggccttcg tcagtcagtg tgaccgagcg
     3301 ggtggtgcgg tcgaatagcc ggatgccgag gcgtgcttcg agtcttgcaa ccgaacggct
     3361 gaccccggat tgcgacatgt cgagcgcctc gccggctgcg gcaaaactgc cgcaatctac
     3421 gatcgccgtc agtacgccca tgccgttcag catgcgctcg tcaaatggca tctgatattc
     3481 cttgttatgg attaatgcgt gacatgctaa cgcgaaacac cgtttgaatt cacgtgccgg
     3541 tgtcgggaaa cgcctacctg tctgaacgaa tcaatgacat cgaatcatga gtgcaatgat
     3601 agccacgcta tcgcgtcaag cgcccgtctg gcgctaatct gtgtggattc gcccgaggca
     3661 cgatgaaacg cggttctcac catgcgtcag gcatatttga cacatcaacg gagaacgcca
     3721 ttatgtcgcg agtttttatt accggttccg ccgatggtct cggtcagatg gccgcgcgtc
     3781 tactcgtcgc cgccggtcat caagtggttt tgcacgcgcg cagtgcggcg cgcgcggagc
     3841 aagcattgaa ggccgtgccg gcagccgaaa ctgctttaat aggcgatttg tcgagcattg
     3901 cagccacggt cgcattggcg gagcaggtca acacactcgg ccgcttcgac gcggtgatcc
     3961 ataacgcggc ggtcggttac caggagccgc gccggatcgc caccctggac ggcctaccgc
     4021 atgtgttcgc ggtcaacacg ctggcccctt acgtattgac cgcgctgatc agaaagccga
     4081 agcggctcgt ctatctgagt tcgggcttgc atcgcagcgg cgacgcgagc ctcgacgatc
     4141 tggcatggga gcggcggcca tggcagggca cggcggccta ttccgactcg aaactgcatg
     4201 acgcgctgct ggcgcttgca atggcgcgcc gctggccgga ggtgttgtcg aacgcgctgg
     4261 agcccggttg ggtggcgacc agaatgggcg gggcgggcgc gccggacgat ctgcaagcgg
     4321 cgccggaaac ccaggtgtgg ctcgctgtta gtgatgaacc ggcggccacc gtgagcggcg
     4381 cctacttcta tcatttgaaa ccgcgcgaga cgcatccggc ggtgcacgac gtcgctgtgc
     4441 aagagcgttt gatcgaggcg tgcgagggat tttccggcgt ccgtttgccg gactgagggc
     4501 ctcgacggca gacagtgcgc tgagtccgcc caggtcacac ctgtctgatt cagcgcacgc
     4561 aaagccagag gcgcgtatgc gaatccacca cgctggcgat cagcgccccg cgccctgggc
     4621 cgcctgtcca cgcactccgc gagcgacagg tgcttcgtcg agatggcgaa agatccacgc
     4681 cgacaccagc gtaatgacgc ccacgcacac aaagctcagg cggaatccga gtgccgccga
     4741 tccccattgc gccgagaaca gattcaccag cccgccgccg atcgaaacgc ccagcccgat
     4801 cgccagcatc tgcaccatcg aaaagaggct gttgccgctc ccggcatctt cgtgcgagag
     4861 acccttaagc gtgacgctgt tcatcgccgc gaactgcatg gagttggccg cgccgaac
//
LOCUS       DATASH010000215         3651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2797224,
            whole genome shotgun sequence.
ACCESSION   DATASH010000215 DATASH010000000
VERSION     DATASH010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2797224"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2954
                     /locus_tag="VHV99_06080"
     CDS             <1..2954
                     /locus_tag="VHV99_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX3635047.1"
                     /translation="FVQIEIHTSRLQARYLSELGRDVAFTLADGASNSIRFPASGPYD
                     LRLGYALEPSFEERLTARGFAVSSQARDSARMLALADDGLFLPYDEKDQAGLLLRDAR
                     GTQLFSARYPNRVYDSFDAIPPVMVNALLFIEDRYLLDASQPNRNPAIDWGRFGRAVF
                     DQGMRVVDRHQPAPGGSTLATQIEKFRHSPGGRTATPPEKLRQIASASVRAYLDGPQT
                     LPARRQIVVRYLNSVPLAAKPGVGEINGMGDGLTGWYGRDFDEVNHILNAPASAETLD
                     AQALAFKQALSLMIAQRAPSHFLRSGDMELDRLTDSYLRLLAANGIISTSLRDSALAV
                     RLELDRAPVVHAPVSFVTRKAVTALRAHLLTALGVSNVYDLDRLDLTATTTLDNRVQQ
                     AVSERLANAATREGAQAAGLYGFEMLRMKDDPSKIAYSFTLFERRDGVNLVRVQTDSV
                     NQPFDINQGARLNLGSTAKLRTLITYLQIVSALHVRYASMSVAQLHDVKPDKQDGLTR
                     WALDYLSHTADRSLSAMLHAAVERKYSASAGETFYTGGGAQTFSNFEASDNGRILTVH
                     RAFQHSVNLVFVRLMRDIVHYEMIQAAGPTSQWLDDPATRRAYLTRFADQESRVYMHR
                     FYVKYRGKTPDTALARLLYDVRKSPPKVATVLRSVAPDEPQVWFDAQMRAALKRAPTA
                     SLSSDDLAKLYAKYGIDRFNLNDRGYVSSVHPLELWTVNYLRDHPLATESQLQEASRA
                     VRSTTYSWLFKTRYHATQDRRIKRMVELQAFAEIGKSWRALGYPFETITPSYAAAIGA
                     SGDRPAALAQLVGTIANGGKMLLAQTFAELEFAKGTPYETHFTRAATTPPTALSPEIV
                     DVVHGLLRDVVLGGTARRLAAGMTFPDGQTLEVYGKTGTGDQRFNVFARGARLIESRK
                     VNRSATFVFVIGDRFFGTLTACVHEPFAARYTYTSALTVQLLKSLTPALQPLLDGDEH
                     SAILSSTAHRE"
     gene            complement(3180..>3651)
                     /locus_tag="VHV99_06085"
     CDS             complement(3180..>3651)
                     /locus_tag="VHV99_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635048.1"
                     /translation="VAIAESHYLVALHVFVTAEAEVIAAFFRRCCRAVAVNDRRIEKL
                     ILVQFGHRTEQYGIQTAIRCPAPENAVDARGMNFGATGRILVDRQRLSLAAQIEHLQN
                     VVEDLAQGQFGHRSTVPTREMRQDKFVELRIAQLHWNAVPRLAFGHLGPKASRF"
BASE COUNT          694 a         1148 c         1105 g          704 t
ORIGIN      
        1 gcttcgttca aattgaaatc catacctccc ggctgcaggc gcgctatctg tccgaactag
       61 ggcgagatgt cgctttcacc ctcgccgatg gcgcgagcaa tagcatccgg tttcctgcga
      121 gtggcccgta cgacctgcgt ctcggttacg cgctggagcc gtccttcgaa gaacgcctta
      181 ctgcacgcgg ttttgccgtc tcctcgcagg cacgcgattc cgccaggatg ttggcgctgg
      241 cagacgatgg gctgttccta ccttacgacg aaaaggatca ggccgggcta ctgctgcgcg
      301 atgcgcgtgg cacacagctt ttcagcgcgc ggtatccgaa tcgggtctac gacagcttcg
      361 acgcgattcc gccagtcatg gtcaacgcgt tgcttttcat cgaagaccgc tacctgctcg
      421 acgcgagcca gccgaaccgc aatccggcaa tcgactgggg ccgtttcggc cgtgcggtgt
      481 tcgatcaggg catgcgcgtg gtcgaccgcc atcagccggc gccgggcggc agcacgctcg
      541 ccacgcagat cgaaaaattc cgccactcgc cgggcggccg caccgcgacg ccgccggaga
      601 agttgcggca aatcgcgtcg gcgtcggtgc gtgcttatct cgacggaccc cagacactgc
      661 ccgcgcgcag gcagattgtc gtgcgctatc tcaactctgt gccgctcgcg gcgaagcccg
      721 gcgtcggcga aatcaacggc atgggcgatg gtctgacggg gtggtacgga cgcgacttcg
      781 acgaggtgaa ccacatcttg aatgcgccgg cttctgccga aacgctcgat gcgcaagcgc
      841 tcgcgttcaa gcaagcgttg tcgctgatga tcgcgcagcg tgcgccgtcg cattttctgc
      901 gttccgggga catggaactc gatcgcctga cggacagcta tctgcgcctc ctggccgcca
      961 acgggatcat ttcgacgtcg ctgcgtgaca gcgcactcgc agtgcggctc gaactggatc
     1021 gcgcgcccgt ggtgcacgca ccggtatcgt tcgttacgcg caaggccgtc accgcgctgc
     1081 gtgcgcatct gctcacggcg ctcggcgtat cgaacgtcta cgacctcgac aggctcgatc
     1141 tgacagctac caccaccctc gacaacaggg tccagcaagc ggtcagcgaa aggctcgcga
     1201 acgccgccac gcgcgagggc gcgcaggccg ccggcctata cggcttcgaa atgctgcgca
     1261 tgaaggacga tccgtcgaag atcgcctaca gctttacgct gttcgagcgt cgcgacgggg
     1321 tgaacctcgt gcgggtccag accgacagcg tgaaccagcc gttcgatatc aatcagggcg
     1381 cacgcctgaa cctcggctcg accgccaagc ttcgcacatt gatcacgtac ttgcagatcg
     1441 tctccgcgct gcatgtgcgt tatgcgtcga tgagcgtggc gcaactgcac gatgtgaagc
     1501 ccgacaagca ggatgggttg acgcgctggg cgctcgacta cctgtcgcac acagcggatc
     1561 gctcgctgag cgcgatgctc catgccgcgg tcgaacgcaa gtattccgca agtgcaggcg
     1621 agaccttcta caccggcggt ggcgcgcaga ctttcagtaa cttcgaagcg agcgacaacg
     1681 gccgcattct caccgtgcat cgcgcgttcc agcattcggt gaatctcgtg tttgtgcggc
     1741 tgatgcgtga catcgtgcac tacgagatga tccaggccgc gggaccgacg tcgcaatggc
     1801 tcgacgatcc tgcaacacgc cgggcttatc tcacgcgttt cgccgatcaa gaaagccgcg
     1861 tgtatatgca tcgtttctat gtgaagtatc gcggcaagac acccgacaca gcgcttgccc
     1921 gcctcctgta tgacgtgcgc aagtcgccgc cgaaggtcgc gaccgtattg cgcagcgtgg
     1981 cgcccgatga accacaagtg tggttcgacg cacagatgcg cgccgcgctg aaacgcgcgc
     2041 ccacggcatc gctttcgtcc gacgatcttg cgaagctcta cgcgaagtac ggcatcgacc
     2101 gattcaattt gaacgatcgg ggctatgtct cgagcgttca tccgctcgaa ctgtggacgg
     2161 tcaattacct gcgggaccat ccgctcgcga ccgagagcca gttgcaggaa gcgagccgcg
     2221 cagtgcggtc caccacgtat tcttggctct tcaagacgcg ttaccacgca acgcaggatc
     2281 ggcgcatcaa gcgcatggtc gaattgcagg ccttcgccga aatcggtaaa tcgtggcgcg
     2341 cgctgggcta tccgttcgag acgatcacgc cgtcgtacgc cgcggcgatc ggtgcgtcgg
     2401 gcgaccggcc ggccgcactc gcacaactcg tcggcacaat tgcgaacggc ggcaagatgc
     2461 tgctggcgca gacctttgcc gaactcgagt tcgcgaaggg cacgccctac gagacgcatt
     2521 tcacgcgggc agcgacgacg ccgcccacgg cgttgtcgcc cgagattgtc gatgtggtgc
     2581 atggcttgtt gcgcgatgtg gtgctcggcg gcacggcgag gcgtcttgca gccggcatga
     2641 cgttccctga cggacagacg ctcgaggtgt atggcaagac gggaacgggc gaccagcgtt
     2701 tcaatgtgtt cgcccgtggt gcgcgcctga tcgagtcgcg caaggtcaac cggagcgcga
     2761 cattcgtctt cgtgatcggc gatcggttct tcggcacgct gaccgcttgc gtgcatgagc
     2821 cctttgcggc gcgctacacc tacacgagtg cgttgacggt gcagttgctg aagtcgctga
     2881 cgcccgcact gcaaccgttg ctggatggcg atgagcattc agcaatccta tcttcgacgg
     2941 cgcaccggga ataaccgcgg cgtgccaata ttcacccgcg aatctgacct atcgcggcga
     3001 atctgcgtac acgtcgtccc cccgacgcat ccgtggtcca gacgacgcca ccatgaactt
     3061 gtttggtcgc acgaacacgg ttttggccaa atccagcaac tggttgcagg ttttcccggc
     3121 acgtgaattt gtctgaaagc ctttgcaggt ctgcctttta tcgatatggg gcgaatccgc
     3181 tagaatctcg atgctttcgg gccgagatgg ccaaaagcca gccgcggcac ggcattccag
     3241 tgcaactgag cgatgcgcag ttcgacgaat ttgtcttgcc tcatctcacg cgtgggcacc
     3301 gtggaccgat gcccaaattg cccttgcgca agatcttcaa ctacattctg aagatgctct
     3361 atctgggctg ccagtgaaag gcgctgccga tcgacaagga tccggccagt cgccccgaaa
     3421 ttcatcccac gagcgtctac cgcattttcc ggcgctggac agcggatggc tgtctggatg
     3481 ccatactgct cggttcggtg tccaaactgc acgaggatca acttctcgat acgtctgtcg
     3541 ttcacggcga cggcacgaca acagcggcga aaaaaggcgg cgataacctc ggcttcagcg
     3601 gtcacaaaca catgaagggc gacaaggtag tggctttctg cgatcgcaac t
//
LOCUS       DATASH010000216         3628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2204867,
            whole genome shotgun sequence.
ACCESSION   DATASH010000216 DATASH010000000
VERSION     DATASH010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2204867"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VHV99_06090"
     CDS             <1..226
                     /locus_tag="VHV99_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635049.1"
                     /translation="QGKAKEPDQGKANAIKAQTNKRRAGKTKSPEAHRRRQTGKAPDR
                     QKNQIFIAAAPDTASTQQHLYPSYRVQRVS"
     gene            complement(308..619)
                     /locus_tag="VHV99_06095"
     CDS             complement(308..619)
                     /locus_tag="VHV99_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4148 domain-containing protein"
                     /protein_id="HEX3635050.1"
                     /translation="MKRNLLAGLALSLLASAPVFAQGNGGGGIGHAGTYDTQPAPSVG
                     GKTRAEVQAELVAAYRDGSLPALNRTTYPNKGLVGQTQAARIALQEDRSGDVTRVANG
                     Q"
     gene            complement(833..1837)
                     /locus_tag="VHV99_06100"
     CDS             complement(833..1837)
                     /locus_tag="VHV99_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006484497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3635051.1"
                     /translation="MDRLQAMQVFTRVVDTNSFTRAAETLDLPRASVTTIIQNLEAFL
                     GTRLMHRTTRRLSLTPDGAAYYERCVRILADVEETEASFQSGNKKPHGKLRIDMPGSI
                     GRLLVIPSLCEFHTRYPDIDLQLGLTDRPVDLLQEGVDCVIRVGALQDSSLVARRIGL
                     FEGVTCAAPDYIERAGMPTSLEDLENHKAVNYFSSRTGRTLDWAFKVDGKEVEVKMKG
                     IVSVNDSDAYVTCGVEGFGLIQPALFMVLPHLRSGQLVEVLPELKPLPMPISAVYPHS
                     RHLSPKVRVFVDWIAEVFDRCPLLSGRGSLDATCTKRTFEERESAPMLDTPVITEWVA
                     "
     gene            2012..2866
                     /locus_tag="VHV99_06105"
     CDS             2012..2866
                     /locus_tag="VHV99_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018423443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3635052.1"
                     /translation="MDAFKPSYSFVPAGSGPAQADSTALDVTDVRIEGHAQDITLRLY
                     RQAEKTALPVLLYFHGGGFTKGSIDQADYAARYFAEHLPALVVSVGYSLAPQFPFPAA
                     PEDAHRAALWVQTRARAFGGNSKKIGVAGHDAGGQLANCLAFIARDRGDVRIAAQALF
                     GPMLDPSLTRLGDEQRLGSDITAKECAACYRAYLPQASQRMHPYAAPLESLRLAGLPA
                     TLIVTAQNDVLHVEAEKYASSLIDAGVLTQVVRYPSISHAALADHPPALQEAVRFFQW
                     RFDARAHR"
     gene            2912..>3628
                     /locus_tag="VHV99_06110"
     CDS             2912..>3628
                     /locus_tag="VHV99_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX3635053.1"
                     /translation="MTILPLSRRRVAIAALAVVVVAGLGTFGAIRVDASSPAAPTIVP
                     EVDVATVVQKTITDWQSYSGRLEAVEKVDVRSQVPGTIVSVNFKDGALVKKGDTLFVI
                     DPRPYAAEVDRAEAQLAAAQARTGYTQSDWERAQRLIADNAIAKRDYDEKQNAAREAS
                     ANLKAAQAAVETARINLGYTKIVAPVAGRVSRAEITVGNVVSAGASSAPLTTLVSVSP
                     IYASFDADEQTYLQYLSRAKD"
BASE COUNT          780 a         1125 c         1054 g          669 t
ORIGIN      
        1 tcaaggaaag gccaaagagc cagatcaagg aaaggccaac gccataaaag cacagacaaa
       61 caagcgccgc gcaggcaaaa caaagtcacc agaagcccac cgccgcaggc aaacaggcaa
      121 agcgccagac aggcaaaaaa accaaatctt tatcgctgcg gcgcccgata cggcgtccac
      181 acagcagcat ctgtatccca gctatcgagt tcagagggtg tcatgatgag atcctttttg
      241 aagaatgcat attcgccacc gcgataacta tttcctttgg aaaccgaatc atcgcggcgg
      301 agccacttta ctgcccatta gcaacgcgcg tcacatcgcc gcttcgatct tcctgcaatg
      361 caatgcgcgc agcctgcgtc tgcccaacca gacctttatt cggatacgtc gtgcgattca
      421 gcgcaggcaa cgatccatcg cgatacgccg cgaccagttc agcctgaact tcagcgcgag
      481 ttttgccgcc aacgctcgga gcaggttgcg tgtcgtacgt gcctgcatgc ccaatgcccc
      541 caccaccgtt gccttgagca aaaaccggcg cgctggcaag cagcgaaaga gccagacctg
      601 ctaagagatt gcgtttcatg attcactcct gtcgattcat ttgcagcaca ggcagcgccg
      661 cgacagagtg aaatgtagac ttcgagccga gtcggataaa tcgcagtttc gcgaaatgaa
      721 ttgtcgcaat tccagaacaa tcgcgacggc catcaagtaa atcaggcaac cgcggcatta
      781 gcgccgcggc tggaattcac tgttccatat tcggtacttc aactcgcgcg aatcacgcga
      841 cccattccgt tatcaccggc gtgtcgagca tcggagcaga ttcgcgctct tcgaaagtgc
      901 gcttggtgca cgtcgcgtcg agactgccgc gtccgctcaa caacgggcag cggtcgaaca
      961 cctcggcaat ccagtctacg aaaacacgga ctttcggcga cagatggcga ctgtgcggat
     1021 agactgcgga aatcggcatc ggcaaaggct tcagctccgg caggacttca accagctgac
     1081 ccgaacgcag atgcggcagc accatgaaga gcgccggctg aatcaggccg aagccttcca
     1141 ctccgcaggt tacgtaggca tcagagtcgt tcaccgacac aatgcccttc attttcactt
     1201 cgacctcttt gccatcaacc ttgaaggccc aatcgagcgt gcgtcccgtg cggctcgaaa
     1261 agtaattgac tgctttgtga ttctcgaggt cctcgagcga agtcggcata cccgcgcgct
     1321 cgatgtagtc gggggccgcg catgtcacgc cctcgaacaa accgatacgc cgcgccacta
     1381 gcgacgaatc ctgtagcgca cccacacgta tcacgcaatc cacgccttcc tgcaacagat
     1441 cgacaggccg gtccgtcaat ccgagttgca ggtcgatgtc cgggtagcgc gtgtggaatt
     1501 cacacagcga aggaatcacc agcaagcgcc cgatcgatcc cggcatgtcg atgcgcagct
     1561 tgccatgcgg cttcttattg ccgctctgga agctggcctc ggtctcttcg acgtccgcga
     1621 ggatgcgcac gcagcgttcg taatacgcgg cgccgtccgg cgtcagcgac aaacgccgcg
     1681 tggtccggtg catcaggcgc gtgccgagga acgcttcgag gttctgaatg atggtggtaa
     1741 cggatgcgcg cggtaaatcg agcgtttccg ctgcacgggt aaagctgttg gtgtcgacga
     1801 cgcgcgtgaa cacttgcatg gcctgaagcc ggtccattgc aaacctccag ataggggcgg
     1861 gcgctctgag cagaaatcga atccgcagag caatcgcatt cgattgttca gggtcgccga
     1921 attgtgttgc cggattatag gcatttattt acgagtctgc cgaacccaca attcgcacca
     1981 ttgaagttct atgcgcattg cgcggctcga catggatgca tttaaaccgt cgtactcctt
     2041 tgtgccggcc ggcagcggcc cggcacaggc tgatagcacg gcactcgacg tcactgacgt
     2101 gcggatcgaa gggcatgcgc aggacatcac gttgcgtttg taccggcagg cagagaaaac
     2161 cgcactgcca gtactgcttt atttccacgg cggcggcttt accaagggct cgatcgatca
     2221 ggccgactac gccgcgcgtt atttcgcaga acacttaccg gctctcgtcg tctcggtcgg
     2281 ttactcgctg gcgccacagt ttccgtttcc ggcggcgcct gaagacgcac atcgcgcggc
     2341 actctgggtg cagacccgag cacgcgcgtt tggcggaaat agcaagaaga tcggcgtggc
     2401 cggccacgat gcgggcggcc aactggccaa ttgcctcgct ttcatcgcgc gtgaccgtgg
     2461 cgatgtgcgc atcgccgcgc aagcgctgtt cggaccgatg ctcgatccga gcttgacgcg
     2521 cctcggcgat gagcaacggc tcggctcgga catcaccgcg aaagagtgcg cggcgtgtta
     2581 tcgcgcatat ctgccgcaag cgtcgcaacg catgcatccg tatgccgcgc cgctcgaatc
     2641 gttgcgcctc gcgggcctgc cggccacgct gatcgtgacg gcgcaaaacg atgtgctgca
     2701 tgtggaagcg gagaaatacg cgagcagtct gatcgacgcg ggtgtgctga cccaggtggt
     2761 ccgctatccc agcatctcgc acgccgcgtt ggccgaccat ccgcccgcct tgcaggaagc
     2821 ggtgagattt tttcaatggc gcttcgatgc gcgcgcccat cgctgaaacg gaaataaaca
     2881 gaactttaat caacgatacc gggagcttct catgactatc cttcctcttt cccgtcgccg
     2941 ggtggcgatt gccgcgctag ccgtcgtcgt cgtcgccggg ctgggcacgt tcggcgcgat
     3001 tcgcgtcgac gcgagctcgc ccgccgcgcc caccatcgtg ccggaagtgg atgtggccac
     3061 cgtggtgcaa aagaccatca ccgactggca gagctattcg ggccgcctcg aagcggtcga
     3121 aaaagtcgac gtccgttcgc aagtgccggg cacgatcgtg tccgtgaact tcaaggacgg
     3181 cgcgctcgtg aagaaaggcg acacgctgtt cgtgatcgat ccgcgtcctt acgcagccga
     3241 agtcgatcgc gccgaagcgc aactcgcggc tgcgcaagcg cgtacgggct acacgcaaag
     3301 cgattgggag cgcgcgcagc gcctgatcgc cgacaacgcc atcgccaaac gcgactacga
     3361 cgagaagcag aacgccgcgc gcgaagcgag cgcgaacctg aaggccgcgc aggccgccgt
     3421 cgaaaccgcg cgcatcaatc tcggctacac gaagatcgtc gcgccggtgg cgggccgcgt
     3481 atcgcgcgcg gaaatcacgg tgggcaatgt ggtgtccgct ggcgcaagct cggcgccgct
     3541 gacgacgctg gtgtcggttt cgccgatcta cgcatcgttc gacgctgacg aacaaaccta
     3601 tctgcagtac ctgagccgcg cgaaggat
//
LOCUS       DATASH010000217         3135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4380722,
            whole genome shotgun sequence.
ACCESSION   DATASH010000217 DATASH010000000
VERSION     DATASH010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4380722"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="VHV99_06115"
     CDS             complement(<1..519)
                     /locus_tag="VHV99_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013337853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HEX3635054.1"
                     /translation="MQVIHTMAKPRSTSKQSKQTGGTFLGIVLGLIVGLAIAVIVALY
                     ITRAPTPFVAKVAPAPSDAGSAPATGQPYDPNRPLQGKTPGQAVPQAAQPAPPNTAPG
                     QTNNQTQSSGVQDEPEILEVPPSAANGMAVAPKPAAPAAVPPVANAPGGSSSATPAKK
                     PADAQASSTAKVT"
     gene            complement(664..2448)
                     /gene="argS"
                     /locus_tag="VHV99_06120"
     CDS             complement(664..2448)
                     /gene="argS"
                     /locus_tag="VHV99_06120"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004522947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX3635055.1"
                     /translation="MLPAQKHTLETLLADAVKQVVQASQGASEANFVAPVIVLERPKV
                     AAHGDVASNVAMQLAKPLRANPRQLAQQIVDAVLALPAAQGLVDAAEVAGPGFINLRL
                     AAAAKQAVVPAVIVEGARYGYNASDEGKRVLIEFVSANPTGPLHVGHGRQATLGDALA
                     NLLGTQGNAVHREFYYNDAGVQIGNLATSTQLRARGFKPDDKEWPENAYNGEYIADIA
                     RDYLAGATVAAKDGAPVTAKGDVDDIDAIRVFAVAYLRHEQDLDLTAFGVDFDQFYLE
                     SSLYNEGRVQATVDALIAAGKTYEQDGALWLRTTDDGDDKDRVMRKSDGTYTYFVPDV
                     AYHVAKWQRGFTKVINVQGSDHHGTIARVRAGLQGLGIGIPKGYPDYVLHKMVTVMRN
                     GVEVKISKRAGSYVTVRDLIEWSGGMTPGEEVAPDQIDVDTIRRGRDAVRFFLISRKA
                     DTEFVFDVDLALKQSDENPVYYVQYAHARICTILGDWKTKYAGNADALPAVDLSPLSS
                     ERAMALLNKLAEYPDMLSHAADELAPHAVAFYLRDLAGEFHSFYNAERVLVDEDGVRN
                     ARIALLAATRQVLENGLALIGVSAPVKM"
     gene            2963..>3135
                     /locus_tag="VHV99_06125"
     CDS             2963..>3135
                     /locus_tag="VHV99_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1840 family protein"
                     /protein_id="HEX3635056.1"
                     /translation="MLITFKTRAAPDVQMLDNLADYLLGIIGKRLGERGVITHEELPE
                     CIQKLESAVATDK"
BASE COUNT          554 a          986 c          969 g          626 t
ORIGIN      
        1 cgtgaccttg gccgtagacg aagcctgtgc atcagccggc ttcttcgcag gcgtcgcgct
       61 cgacgagcca cctggcgcgt tcgcgaccgg cggcacagcg gccggagcgg ctggcttcgg
      121 cgcaacggcc ataccgttcg cagccgatgg cggcacttcc agaatctccg gttcgtcctg
      181 tacgcccgac gactgcgtct ggttgttcgt ctgtcctggc gcggtgttcg gcggcgcggg
      241 ctgcgcggct tgcggcacgg cctggcccgg cgtcttgccc tgcagcggcc ggttcgggtc
      301 gtacggctgt cccgtggcag gcgcggagcc cgcgtcagat ggcgctggcg ctaccttcgc
      361 gacaaacggc gtgggggcgc gcgtgatgta caacgccacg atcactgcaa ttgcaaggcc
      421 gacaatcaag cccagcacga taccgagaaa agtgcccccg gtttgcttcg actgcttcga
      481 cgtgctgcgt ggttttgcca tcgtatgaat aacctgcaaa aagtcttctg aatggccgtc
      541 gattatagcg gcggccttaa gcgcggtttg gcgttgcgcg gtgttgtgtt gcgcggtgtt
      601 gcactacgtc atgcactcgt cgtcagccgg cttcacccgc accggaggtg cccggcacgg
      661 gaatcacatc ttgacggggg cggacacgcc gatcagcgcg aggccgttct cgagcacttg
      721 cctcgtagcg gcgagcaacg caatgcgcgc attgcgcacg ccgtcttcgt ccaccagcac
      781 gcgctccgca ttgtagaacg agtggaattc gcccgcgagg tcgcgcagat agaacgcgac
      841 cgcgtgcggc gcgagttcat cggcggcgtg agacagcatg tccggatatt cggcgagctt
      901 gttcagcagc gccatcgcac gttcgctcga gagcggcgag agatcgacgg ccggcaaagc
      961 gtccgcattg ccggcgtatt tcgtcttcca gtcgccgagg atcgtgcaga tccgcgcgtg
     1021 cgcgtactgc acgtagtaca ccgggttctc gtcgctttgc ttgagcgcca ggtcaacatc
     1081 gaagacaaat tccgtatcgg ccttacgcga gatcaggaag aaacgcacgg cgtcgcgacc
     1141 gcggcgaatg gtgtctacat cgatctgatc cggcgcgact tcctcgcccg gcgtcatgcc
     1201 gcccgaccat tcgatcaggt cgcgcaccgt cacatagctg cccgcgcgct tcgagatctt
     1261 cacttccacc ccgttgcgca tgaccgtgac cattttgtgc agcacgtagt cggggtagcc
     1321 cttcggaatg ccgatgccaa gcccctgcag ccccgcccgc acgcgcgcga tggtgccgtg
     1381 gtggtccgag ccctgcacgt tgatgacctt ggtgaaaccg cgctgccact tcgccacgtg
     1441 gtacgcgacg tccggcacga aatacgtata ggtgccgtcg gacttgcgca ttacgcggtc
     1501 cttgtcgtcg ccgtcatcgg tcgtgcgcaa ccatagcgcg ccgtcctgct cgtaggtttt
     1561 gccggcggcg atcagcgcat cgaccgtggc ttgcacgcgg ccttcgttat acagcgacga
     1621 ctccagatag aactggtcga agtccacgcc aaacgcggtc aggtccagat cctgttcgtg
     1681 gcgcagatac gcgacggcaa agacgcgaat agcgtcaatg tcgtccacat cgcccttcgc
     1741 cgtcaccggc gcaccgtcct tggcggccac cgtcgcgccg gccagatagt cacgtgcgat
     1801 atccgcgatg tactcgccgt tgtacgcgtt ctccggccat tccttgtcgt ctggcttgaa
     1861 accacgcgcg cgcaattgcg tggacgtggc cagattgccg atctgcacgc cggcgtcgtt
     1921 gtagtagaac tcgcgatgca ccgcgttgcc ttgcgtgccg agcaggttcg ccagcgcgtc
     1981 gccgagcgtg gcttgccggc cgtgacctac gtgcagcggc ccggtcgggt tcgccgagac
     2041 aaactcgatc agcacgcgct tgccttcgtc gctggcgttg tagccgtagc gcgcgccttc
     2101 cacaatcacc gccggcacca cggcctgttt cgccgctgcc gccagccgca agttgatgaa
     2161 gcccggaccg gcgacttcgg ctgcatcgac cagaccctga gcggctggca gcgcgagcac
     2221 tgcatccaca atttgctgcg ccagttggcg tggattcgcg cgcagcggct tggcgagctg
     2281 catggccacg ttcgacgcca cgtcaccgtg cgccgcgact ttcgggcgct ccaggacgat
     2341 gaccggcgcg acaaagttcg cttccgatgc gccttgcgac gcctgtacga cctgcttgac
     2401 cgcgtcggcg agcagcgttt cgagtgtgtg tttttgtgcg ggcagcatgc tgattcttca
     2461 atccggtaat agggctgttg gtccgatgca gcgatgcttc gttccttcga cgtccaggct
     2521 tcaatccatg cgtttgccag gaatgcggga aaaacgtgcg gcggagcgtt atacgccgac
     2581 gtgatccgtg ctcaaaaatt tcagtccagt ttcaatccgt tacgtcccat ttacgctcgt
     2641 tccgctggtt cgggcgcggg agaaaccctc ctcgatacac ggtcaggacg ctacgcacgc
     2701 ctgcatattc gtgtccggag gcataaccgg ggccggttac agcgataccg ccaaaaccgg
     2761 ggttttgtaa ccgacgcaac atttgctcgg gtaaatcggg attttagcag gtgctaatat
     2821 gtcgaatcag ggtcgagtcg gggaaagaag tcgggaatta aaacaatgcg ccggcgtgtg
     2881 atgtggtggc ctgggttgtt accacgcttg gcgcactcgc tcactagcca caaaaggcca
     2941 acaaaaggga aaggaccttg ccatgttgat cacattcaaa accagagctg caccagacgt
     3001 tcagatgctc gacaacctcg ctgattactt gcttggcatc atcggcaagc ggcttgggga
     3061 gcgcggcgtc attacgcacg aagaactgcc tgaatgcatc cagaagctgg agtcggcggt
     3121 tgcaaccgac aaaca
//
LOCUS       DATASH010000218         3719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3984551,
            whole genome shotgun sequence.
ACCESSION   DATASH010000218 DATASH010000000
VERSION     DATASH010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3984551"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(280..1161)
                     /locus_tag="VHV99_06130"
     CDS             complement(280..1161)
                     /locus_tag="VHV99_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018431741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HEX3635057.1"
                     /translation="MSLHENHLTLATAQASAHSQWRARLELGFVKDGERTTLKHRLHD
                     GPLRVQRPLYPEGHGICHAVIVHPPGGVAGGDRLAIDVDVGAGAHAVITTPGATKWYK
                     SNGQEARQQIDVRVGERARLDWLPQNNIVFDHANAKLDFCLTLAEGATAIGWDATQLG
                     RQAAGERWSEGSLGAVSRIVGANGELLWFERSVLAASDSLRDAAQGLAGFPAYGTLWA
                     VGAACDDALTESLTAQLPFDDAVRAGASCVTSGVLLVRAVSRSMEQLQRAMTQCWLQL
                     RPVVHAVEAVPLRIWST"
     gene            complement(1278..1970)
                     /gene="urtE"
                     /locus_tag="VHV99_06135"
     CDS             complement(1278..1970)
                     /gene="urtE"
                     /locus_tag="VHV99_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017332923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HEX3635058.1"
                     /translation="MLEVENLNQYYGGSHILRDVKLTVPDGKLTVLLGRNGVGKTTLL
                     RCLMGVVQTKSGSIAWRGAPITKLPTYSRVEKGLAYVPQGRDIFPRLTVEENLMVGAA
                     SKKAPKNIPDRIYELFPVLKDMRSRRGGDLSGGQQQQLAIGRALMSDPQLLILDEPTE
                     GIQPSIIQDIGRTLRQLVEEMGMTVLLVEQYYDFAKEIADRYWVMSRGEIIAGGEGAD
                     MDADGVRTLIAV"
     gene            complement(2030..2893)
                     /gene="urtD"
                     /locus_tag="VHV99_06140"
     CDS             complement(2030..2893)
                     /gene="urtD"
                     /locus_tag="VHV99_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006025537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HEX3635059.1"
                     /translation="MNRNPIVPDLALPEEPAETSLSGVASMGRAVVPGEIDVSHGTIL
                     YLEDVTVSFDGFRALNALTLTIDAGELRCIIGPNGAGKTTMMDVITGKTEPDSGKVFL
                     GQSIDLTRMNEPSIARAGIGRKFQKPTVFEQHPVWENLELAMKADKGWWASLRARLDR
                     EAQARIEETLALIGLESEARRLAGELSHGQKQRLEIGMLLMQQPALLLLDEPAAGMTD
                     DETMQLAELLNHLRGTCSMMVVEHDMEFVAALSGETGKVTVMAEGRVLAHGTLDEVKR
                     DATVIESYLGR"
     gene            complement(2890..>3719)
                     /gene="urtC"
                     /locus_tag="VHV99_06145"
     CDS             complement(2890..>3719)
                     /gene="urtC"
                     /locus_tag="VHV99_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015001901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HEX3635060.1"
                     /translation="MYLMRSIGHDGKYGSDLPDFMVFLDWHQLPWYWHGTQHLGYALL
                     LVVLVPAVVAWVFGFFTFRSRVKGVYLSIITQAMTFAAMLLFYRNETGFGGNNGFTDF
                     KRIGGFPITHPGTRTALLLITFAVLILAFIGARAIVTSKLGRVVTAVRDGETRLMFLG
                     YSPLAYKLFVWTVSAILCGIAGALYVPQVGIINPGEMSPGNSIEMAIWVAVGGRGTLI
                     GPIIGAFAVNGAKSFFTANFPEYWLFFLGLIFVLVPLLLPNGIMGLIELVTRKRNRT"
BASE COUNT          807 a         1215 c         1022 g          675 t
ORIGIN      
        1 gggaaatttc agcgcggatg cgccgatggg gaattcgatg atgcaaggcg catctgtcaa
       61 attcggttac cgcgcggacg cgaagcggtc cgatgcggtg gaatgtgctg ccgatctgct
      121 aacgaggcag tcagaagcca agtgcggagt ttaccggcac cttgcggaat ctgaattgcc
      181 gcagaccggg tcctccgaat gcaaatgaaa aggcgtgacc tcaaggaaag gccaacacca
      241 tccggagcac agccggtgtc aaaacagaac cgacgaacct caagtactcc agatccgcaa
      301 aggcacagcc tcaaccgcat gcacaaccgg ccgcaactgc aaccagcact gagtcatcgc
      361 gcgctgcaac tgctccatag accgcgaaac agcgcgcacg agcagcaccc ccgaagtcac
      421 gcaagaagcc ccagcccgca ctgcatcatc aaacggaagc tgagcagtca gcgactcggt
      481 aagcgcatca tcacaagccg ccccaacagc ccaaagcgtt ccataagcgg gaaacccagc
      541 caacccttgc gcggcatcgc gtaaagagtc actcgctgcg agaaccgagc gttcgaacca
      601 cagcaactca ccatttgcgc cgacgatccg cgacacagca cctaagctac cctctgacca
      661 ccgctccccc gcagcctgcc gcccaagctg ggttgcatcc cacccaatcg cagtcgcacc
      721 ttcagcaagc gtcaaacaaa aatcgagctt cgcattcgca tgatcgaaaa cgatattgtt
      781 ctgcggcagc caatcgagcc tggctcgctc gccgacgcgt acgtcgatct gctgccgcgc
      841 ttcctgccca ttggacttgt accatttggt tgccccaggc gtcgtaataa cggcatgcgc
      901 gcccgcacca acgtcaacat caatagcaag ccgatcccca cccgcgaccc cacccggcgg
      961 atgcacgatc accgcatgac agataccgtg accttcggga taaagcggcc tctgaacccg
     1021 caacggccca tcatgcagcc gatgcttgag cgtggtccgc tcaccatcct tgacgaatcc
     1081 aagttcaaga cgtgcgcgcc attgcgaatg agcactggct tgcgcggtgg cgagcgtaag
     1141 gtgattttcg tgaagcgaca tgcgaatgga atgcgaccgg acgaagagaa acaaagcgtg
     1201 cctgcgcacg tcagacaacg ctcaaccgcg ctgccacagg atgcggcgag aatagcacgc
     1261 ccgcttgtgc gtgccgctca cacggcgatc aatgtccgca cgccatctgc atccatgtcg
     1321 gcgccttcgc cgcccgcaat gatctcgcca cggctcatca cccaatatcg atcggcgatc
     1381 tccttggcga agtcgtaata ctgctcgacg agcagcaccg tcatgcccat ttcctcgacc
     1441 agttgacgca gcgtgcggcc aatgtcctga atgatcgacg gctgaatgcc ctcggttggt
     1501 tcgtcgagaa tcagcagttg cgggtcgctc atcaacgcgc ggccaattgc aagctgctgc
     1561 tgctgaccgc ccgagagatc gccgcctcgg cggctgcgca tgtcctttag caccgggaac
     1621 agttcgtaga tacgatcggg gatattcttg ggcgccttct tgctggcggc gcccaccatc
     1681 agattttctt cgacggtcag ccgcggaaaa atatcgcggc cctgcggcac atacgcgagg
     1741 cccttttcca cgcgcgagta ggtcggcagt ttggtgatcg gcgcgccgcg ccaggcgatc
     1801 gagccgcttt tcgtttgcac gacgcccatg aggcaacgca gcagcgtggt cttgcccacg
     1861 ccgttgcggc ccagcagaac ggtgagcttg ccgtcgggca cggtcagttt cacatcccgc
     1921 aaaatgtggc tgccgccgta gtactggttg agattttcta cttctagcat gtgcgttcga
     1981 ccttttcgtt tcttcggccc gcttcattgg cccgctttcc cgatctgctt taccgaccca
     2041 gatacgactc gatgaccgtc gcgtcgcgct tcacttcgtc gagcgtgccg tgagcgagca
     2101 cgcgtccctc ggccatcacc gtgaccttgc ctgtctcgcc tgacaacgcc gccacgaact
     2161 ccatatcgtg ctcgacgacc atcatcgaac atgtgccgcg cagatgattg agcaattcgg
     2221 cgagctgcat ggtttcgtcg tcggtcatgc cggcggcagg ttcatcgagc agcagcagcg
     2281 ccggttgctg catcagcagc atgccgattt caagccgttg tttctgaccg tgcgagagtt
     2341 cgccggcaag ccgccgtgct tcactctcca aaccgataag cgctaacgtt tcttcgatac
     2401 gcgcttgcgc ctcgcgatcc agacgcgcgc gcaacgaagc ccaccagccc ttgtcggcct
     2461 tcatcgccag ttcgaggttt tcccacacgg gatgctgctc aaaaacagtc ggcttctgaa
     2521 acttccgccc aatgcccgcg cgagcaatag acggctcgtt catccgcgtc aaatcgatcg
     2581 actgccccag aaaaaccttg ccggaatccg gctcggtctt cccggtgatg acgtccatca
     2641 tcgtcgtctt gcccgcgccg ttcggcccga tgatgcagcg caattccccg gcgtcgatag
     2701 tcagcgtcag tgcattcaac gcacgaaaac cgtcgaagct cacggtcacg tcttcgaggt
     2761 acaggatcgt gccgtgcgac acgtcgatct cgccgggcac gaccgcacgg cccatgctcg
     2821 cgacgccgct caacgaggtt tcagcgggtt cttcaggcaa cgcaagatct ggaactatcg
     2881 ggtttcggtt catgtgcggt tcctcttgcg cgtcacgagt tcgatcagcc ccatgatgcc
     2941 gttcggcaat aacagcggga ccagcacgaa aatcagaccc aggaagaaca gccagtattc
     3001 agggaagttc gccgtgaaaa agctcttcgc gccattcacc gcgaacgcgc cgataatcgg
     3061 gccaatcaac gtgccgcgtc cgcccaccgc aacccagatc gccatctcga tcgagttgcc
     3121 cggcgacatc tcgcccgggt tgatgatgcc gacctgcggc acgtacaacg cgccggcaat
     3181 cccgcacaaa atagcggaca ccgtccacac gaacagcttg tacgcgagcg ggctgtagcc
     3241 aaggaacatc agacgcgttt cgccgtcgcg caccgcggtg accacgcggc cgagcttcga
     3301 ggtgacgatt gcccgcgcgc caatgaaggc cagaatcaac acggcgaacg tgatgagcag
     3361 cagagcagtg cgcgtacctg gatgcgtgat gggaaaaccg ccgatgcgct tgaagtcggt
     3421 gaagccgtta ttgccgccga atccggtttc gttgcggtag aacagcagca tggccgcgaa
     3481 cgtcatcgcc tgcgtgatga tcgacagata gacgcctttc acccgcgagc ggaacgtgaa
     3541 gaagccgaac acccacgcga caacggcggg caccagcacg acgagcagga gcgcgtagcc
     3601 gagatgttgc gtgccgtgcc agtaccacgg caattgatgc cagtcgagaa acaccatgaa
     3661 gtcgggcaga tcgctaccgt atttgccgtc gtgtccgatc gatcgcatca ggtacatgc
//
LOCUS       DATASH010000219         1993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6753598,
            whole genome shotgun sequence.
ACCESSION   DATASH010000219 DATASH010000000
VERSION     DATASH010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6753598"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1993
                     /locus_tag="VHV99_06150"
     CDS             <1..>1993
                     /locus_tag="VHV99_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEX3635061.1"
                     /translation="VLQHVTRMRVSQVSGPTPIDANHVYVIAPSKSLTMTDGYLNARD
                     AEPMAGRPVTIDRFFRSLAEAHGQHAMCIVMSGTGSDGAVGIATVKERGGVTLAQEPN
                     DAEYAEMPQNAILTDQVDLVMPVVEMPQRLLDIWNNAKRIALPELEPASLAPAGRTDR
                     RSEAELALQAILSTLRARTGHDFRFYKRATMLRRIERRLQVNGVPDLIAYRDLLRDNS
                     RETGALLKDMLIGVTSFFRDREAFESLERGVLPALFHGKSEGDQVRAWVAGCSSGEEA
                     YSLAMLLCEQRDALRSTANIQLFATDIDESAVARARAGLYPESIMTDVRPSMLRQFFI
                     KSDSRYEVVKSVREKILFAMHNVLRDPPFSRLDLVSCRNLLIYLDRGVQNQVLEMFHY
                     ALYPNGYLFLGSSESADSVEDLFSVVDKKNRVYRAKAAKLPNRPSLSSPSLLMQGRFT
                     HDDEAGSALVPGRRNFSFSALHQRVLEQYAPPSVIVNRDSKIVHMSDNAGRFLRYVGG
                     EPSSNMIAVVLPELRLDLRTALFQAAQTGNSVEARRVKLQRDGHPSYVNMTVRAFHDA
                     AADADFLLVLFDEVQEAMTETKPVAEHASGNDSVMQQLEQELHSSKEQLQTTIEQYET
                     STEELKASNEELQAINEELRSATEELETSKEELQSVNEEL"
BASE COUNT          395 a          611 c          646 g          341 t
ORIGIN      
        1 aggtcctgca gcacgtgacc cgcatgcggg tgtcgcaggt cagcggaccg acgccgatag
       61 acgcgaacca tgtctatgtc atcgcgccga gcaagagcct gacgatgacg gacggttacc
      121 tgaacgcgcg cgacgccgag cccatggcgg gccggccggt caccatcgac cggttcttca
      181 ggagcctggc cgaggcgcat ggccagcacg cgatgtgcat cgtgatgtcc gggacgggca
      241 gcgatggcgc ggtcggtatc gccaccgtga aggagcgggg cggcgtgacg ctggcgcagg
      301 aaccgaacga cgccgaatac gccgagatgc cgcagaacgc gatcctgaca gaccaggtcg
      361 acctggtcat gcccgtggtg gagatgccgc aacggctgct ggacatatgg aataacgcca
      421 agcgaatcgc gctgcccgaa ctggaacccg cgtcgcttgc gccggccggc cggaccgacc
      481 gccgcagcga agccgaactc gcgttgcaag ccattctgtc gacgttgcgc gcccgcactg
      541 gtcacgattt ccgcttctac aagcgcgcga ccatgctgcg ccggatcgaa cggcgcctgc
      601 aggtcaacgg tgtaccggac ctgattgctt accgcgatct gctgcgggat aactcgcgcg
      661 aaaccggcgc tttgctcaag gacatgctga ttggcgtgac cagctttttc cgcgatagag
      721 aagcgttcga gtcactcgaa cgcggcgtgc tccctgcgct gttccacggc aagagcgagg
      781 gcgatcaggt gcgcgcgtgg gtcgcgggat gctcgtcggg cgaagaagcg tattcgcttg
      841 ccatgctgtt gtgcgagcag cgcgatgcgc tgcgttccac cgccaacatc cagctgttcg
      901 ccaccgacat cgacgaaagc gccgtcgcgc gggcacgggc cggactgtat cccgagtcga
      961 tcatgaccga cgtccgcccc tccatgctgc ggcaattttt catcaagtcc gactcgcgct
     1021 acgaagtggt gaaaagcgtg cgcgaaaaga tcctgttcgc catgcacaat gtgctgcgcg
     1081 atccgccgtt ctcgcggctc gacctggtct cctgccgcaa tctgctgatc tatctggatc
     1141 gcggcgtgca gaaccaggtg ctggaaatgt tccactacgc gctgtacccc aacgggtatc
     1201 tgttcctggg cagttcggag tcggcggact cggtcgaaga cctgttcagc gtggtcgaca
     1261 agaagaaccg cgtgtatcgg gcgaaggcgg cgaagctgcc gaaccgcccg tcgctgagct
     1321 cgccgtcgtt gctgatgcaa gggcgcttta cgcatgacga cgaggcgggg tccgcgctgg
     1381 ttccggggcg ccgcaatttc tcgttcagcg cgttgcacca gcgtgtgctc gagcaatacg
     1441 cgccgcccag cgtgatcgtc aatcgcgatt cgaagattgt gcatatgtcg gacaacgcgg
     1501 gacgtttcct gcggtatgtc ggcggcgagc cttccagcaa catgattgcc gtcgtgctgc
     1561 ccgaactgcg gctcgacctg cgtacggccc tgttccaggc cgcgcagaca ggcaatagcg
     1621 tggaggctcg gcgggtgaag ctgcagcgcg acggtcaccc gtcctacgtg aacatgaccg
     1681 tgcgcgcctt ccacgacgcc gccgccgacg ccgacttcct cctcgtcctg ttcgacgagg
     1741 tccaggaagc gatgacggaa acgaagccgg tcgcggagca tgcgtctggc aatgactccg
     1801 tgatgcagca gctcgaacaa gagctgcatt cgagcaagga acaactgcag accacgatcg
     1861 agcaatacga aacgtcgacg gaagagttga aggcgtcgaa tgaagaattg caggcgatca
     1921 acgaagagtt gagatcggcg acggaggaac tcgaaaccag caaggaagag ctgcaatcgg
     1981 tcaacgagga act
//
LOCUS       DATASH010000220         9536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5370242,
            whole genome shotgun sequence.
ACCESSION   DATASH010000220 DATASH010000000
VERSION     DATASH010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 9536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9536
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5370242"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..547
                     /locus_tag="VHV99_06155"
     CDS             179..547
                     /locus_tag="VHV99_06155"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3635062.1"
                     /translation="MAANEQSAAKPGTLKQAQALADAGDLLEAAEIARTCLKAHPASA
                     DAYYLLGVIADARGEQSEARGFYKRALYLDPGHGEALTHLAAVLRLEGDTAGSQLLLA
                     RAERAERAERAERASGGRHE"
     gene            540..1382
                     /locus_tag="VHV99_06160"
     CDS             540..1382
                     /locus_tag="VHV99_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX3635063.1"
                     /translation="MSDPRDQRDVRFAADVDAASASDEPSSLFADMDAPIDDCWNRIG
                     VRGDGSCPRLAGYIHCRNCPVHDAAAARVLDREQSADAAIGQEILHRQTAQSLSESAA
                     READATSWLVFRIGDEWLGLPTAIFQQVAQLRPIHSLPHRRHRAVLGVVNVRGSLLVC
                     ASLARLFGIASVVGQDGRRAAQQSGQQGIDARDLARLLVIEPGRDAGERGGSGNSGDP
                     IVFPVDAVDGVHRFARADFQAVPATIASMSASHAVAVTTCNGVTVGLLDAAKLFETLN
                     RSFT"
     gene            1379..3739
                     /locus_tag="VHV99_06165"
     CDS             1379..3739
                     /locus_tag="VHV99_06165"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016355239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEX3635064.1"
                     /translation="MSGAETGQGPSRGQPSLIDLFREEARTQAQILSDGLLALERAPR
                     DHVTLEACMRAAHSLKGAARIVGVPDGVSLAHVMEDCFVGAQQGRVSVDAGMIDVLLG
                     GVDLILRIGQGSGDESLSQTDVDAFVDALNARMDGVTTAPPPSVFEAPQADADVDLSH
                     ELTLSVPLGEATQETVEAAPFQPVRSTELREPARMLRVRADTLDRLLSHSGESLVESR
                     WLKPFAQSMLRVKRIQRDASRALDFLHESLTGAATSASTSSAAPSAALDARTLDALDE
                     VRRLNAEAHRQLGERLAELENCDRRSTHLSQQLYDEALECRMRPFVDATGGFARMVRD
                     VARSLDKQVRFVIAGEATEVDRDILDLLEAPLGHLLRNAVDHGIEAPSLRRALGKPAE
                     GTITLEARHSAGSLFISVSDDGGGVDLDALRRAIVQKRLASETTAARLSDAELLEFLF
                     LPGFSMRDTVTDVSGRGVGLDAVQDVVRQVRGNLRVTQEVLVGTRFVMQLPLTLSVIR
                     SLIVEVDGEPYGLPLAHVTRTLVLPRASIDLLEGHQHFAFEGRRLGIVTAHQILRSGT
                     FETASDTLNVVVIGQGAACYGVVVDRFLGERMLVVQSLDKRLGKVPNIAAGALMENGD
                     PLLIADLDDWLRAVEKLVVDGELGRVSSGTASVIAASRKRVLVVDDSLTVRELERKLL
                     AGRGYDVTVAVDGMDGWNAVRSERFNLVITDIDMPRLDGIELVTLIRRDPQLRETPLM
                     IVSYKDRAEDRQRGLDAGADYYLAKGSFHDQTLLDAVRDLIGEAQS"
     gene            3765..4781
                     /locus_tag="VHV99_06170"
     CDS             3765..4781
                     /locus_tag="VHV99_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008352791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HEX3635065.1"
                     /translation="MKIGIVNDMPLAVDALRAALATRRDFEVVWVARDGAQAVDYCMA
                     QKPDVVLMDLVMPHMDGTEATRRIMRETPCAILIVTVDVGANAWRVYEAMGAGALDAV
                     DTPVLAGPDAKRGIAALTAKIDQIAAQQATKATANPITTAPRITSDTDLLAIGASAGG
                     PGALATLLAALPADFAPATVIVQHVDQAFAIGMADWLNEQSTLPVRVALEGDRPEPGC
                     VLLAATNDHLHFRGGGNRLGYTKEPLATPYRPSIDVFFQSVVARWSGNAVGVLLTGMG
                     RDGAAGLAAMRAKGYHTIAQDEATSAVYGMPKAAAALNAAVAILPLPNIANAVQKAFT
                     SSRK"
     gene            4816..5943
                     /locus_tag="VHV99_06175"
     CDS             4816..5943
                     /locus_tag="VHV99_06175"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008352792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX3635066.1"
                     /translation="MEHRNADPLAERARREQAGPESRPESPARPLDATGEERSAADLL
                     ADALNAPSAADSAVMVLLVDDQTIVAEAVRRALVDQPRLDFHYCANPEDALRVAQATR
                     PTVILQDLVMPGVDGLTLVRQYRENEMTRDIPIIVLSTKEEPRIKSAAFAAGANDYLV
                     KLPDTIELIARIRYHSRSYLNLLQRDEAYRALRMSQQKLLETNLELQRLTNSDGLTGL
                     SNRRCLDDYMNAEWKRAAREQSPLGFLMIDVDNFKSYNDTYGHVAGDDVLKLVARAIE
                     SSLNRPADLAARFGGEEFAVVLPSTSTGGLRLLGEKIRMAVEALAVPHTGSASDRLTI
                     SVGGVMQVPQPGEDMKQMIEAADLALYRAKRDGRNRVEIAN"
     gene            complement(7285..8760)
                     /locus_tag="VHV99_06180"
     CDS             complement(7285..8760)
                     /locus_tag="VHV99_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEX3635067.1"
                     /translation="MTRSALTALGFAAALSACSFGPDGKPPVMPTVAHYGADAQPTRT
                     VAAEGISQSFDAGAKPVPEWWKLYRSEPLNALVDEGLRNSPSLAATDKTLAAAREQLR
                     AQVGESMLPSIDLGGQANRQRAIGVPNFGPPTVLYNLFVGQVRAQYTFDLFGAARLAD
                     RALAAQVDSQSWQFDAARRALAANIVTGAIGASSLRAQIDTTERLIVLANEDARETQR
                     RYELGSASRSDALNAQSNAAALAASLPALRAQWLATRHALAVMIGRTPDQAPPDLDLA
                     SLTVPGNLPVVVPSELLASRPDIQAADALLKAAAAQVGVATAQMFPSLTLSASMGKAG
                     FSWPTALSGAGAIWGIGASLSQPLFHGGALLAQKRAAQASYEAAVAQYKQTVLSAFQN
                     VADSLAALEQDNQALSFADTASRTSMSAFTDSAARERLGAIPPSASRASERQYRNARL
                     DAIRYESARLSDTALLFQAMGSPAGGATNLAAAAKATEAAK"
     gene            complement(8757..9536)
                     /locus_tag="VHV99_06185"
                     /pseudo
     CDS             complement(8757..9536)
                     /locus_tag="VHV99_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389187.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
BASE COUNT         1694 a         2947 c         3164 g         1731 t
ORIGIN      
        1 ggagcgggca atggaatttg ctgcgcgagc agcgccgcca aaggcgctgc tcgcggcggc
       61 cagcgtggca catacgtttg gccctcgagt cgtgccggcc acgaatctga acgatgcacc
      121 tggacgctca ttttcattcg gcttgcctgc tgccggcgcg ccgcgcgcag cgcccgccat
      181 ggcggccaac gaacaatctg ccgccaaacc gggcacgctc aaacaagccc aggcacttgc
      241 cgatgctggc gacctgctcg aagccgccga gattgcgcgg acctgtctca aggcgcatcc
      301 ggccagcgcc gacgcctact acttgctcgg cgtgatcgct gatgcgcgag gtgagcaaag
      361 cgaggcgcga ggtttttaca aacgcgcgct ttatcttgac cccgggcatg gcgaggcgct
      421 gacgcatctt gccgccgtgc tcaggctgga aggcgatact gcgggctcgc agttgttgct
      481 ggcccgcgca gaacgtgccg aacgtgccga acgtgccgaa cgtgcatcag gaggtcgcca
      541 tgagtgatcc gcgcgaccag cgagacgtga ggtttgcagc agatgtggat gcggcttcag
      601 cgtcggacga gccgtcgtca ttgtttgccg atatggatgc tccgatcgac gactgctgga
      661 accgcatcgg cgttcgcggc gatggttcgt gtccgcgcct ggccggctac attcattgcc
      721 gcaattgtcc tgtgcacgat gcggctgcgg cccgcgtgct ggaccgtgaa caatccgccg
      781 atgccgcgat cggacaagag atcctgcatc gccagacagc gcagtcgctg agcgaatccg
      841 cggcccgcga ggcggatgcc acgtcgtggc tcgtatttcg catcggcgat gaatggctgg
      901 gtctgcccac cgcgatcttc cagcaggtcg cccagttgcg cccgattcac tcgttgccgc
      961 accggcgtca tcgcgcggtg ctcggcgtgg tgaacgtgcg cggctcgttg ctcgtttgtg
     1021 cgtcgctggc acggctgttc gggatcgcgt cggtggtggg gcaggatgga cgcagggccg
     1081 cgcagcagag cggtcagcag ggtatcgatg cccgggatct tgcacgcttg ctggtcattg
     1141 aaccgggcag ggacgctggc gagcgcggtg gttccggcaa ctccggcgac ccgatcgtgt
     1201 tccccgtcga tgcagtcgat ggcgtgcatc gtttcgcgcg ggcggatttc caggcggtgc
     1261 cggcgaccat cgccagcatg tcggcgtccc acgcggtggc cgtgacgacg tgcaatggcg
     1321 tgacggtcgg tctgctcgac gcggctaaac tcttcgagac cttgaaccgg agtttcacat
     1381 gagcggcgct gaaacggggc aggggccaag ccgcggccag ccgtcgctca tcgacttgtt
     1441 tcgcgaggaa gcgcggacgc aggctcagat tctgagcgat ggcctgctcg cgctggaacg
     1501 cgccccgcgc gaccacgtga cgctcgaagc ctgcatgcgt gcggcgcact cgctgaaggg
     1561 cgcggcgcgg attgtcggcg tacccgacgg cgtgagccta gcgcatgtga tggaagactg
     1621 cttcgtcggc gcacaacaag gtcgcgtgag cgtggacgcc gggatgatcg acgtgctgct
     1681 gggcggcgtc gacctgatct tgcggatcgg ccaagggtcc ggcgacgaaa gtttgtcgca
     1741 gacggacgtg gacgcattcg tcgacgcatt gaatgcacgg atggacggcg tgaccacggc
     1801 tccgcctcca tccgtttttg aagcgcctca ggctgacgct gacgtcgatc tatcgcatga
     1861 gctgacgctc tcagtgccgc tgggcgaggc aacacaggaa accgtggaag ctgcgccctt
     1921 ccagccggtc cgttccactg aactgcgtga gccggctcgc atgctgcgcg tccgtgctga
     1981 cacgcttgac cgtttgctga gtcattcggg cgaatcgctg gtcgagtcgc gctggcttaa
     2041 acccttcgcc caatccatgt tgcgcgtgaa gcgaatccag cgagacgcaa gccgggcgct
     2101 cgatttcctg cacgaatcgc taaccggggc ggcgacttcc gcctcgactt catcggcagc
     2161 tccgagcgcg gcgctcgacg cgcgcacgct cgatgcgctg gatgaagtcc gccgtctcaa
     2221 cgcggaggcg catcgccagc tcggcgagcg cctggccgag ctggaaaact gcgaccggcg
     2281 ctcgacgcat ctgtcgcagc aactctacga cgaggcgctc gaatgccgga tgcggccttt
     2341 cgtcgatgcc accggcggtt ttgcgcgcat ggtccgcgac gtcgcgcgtt cgctcgataa
     2401 acaagtgcgc ttcgtgatcg ccggtgaagc gaccgaagtg gaccgcgaca tcctcgacct
     2461 gctcgaagcg ccgctgggtc atttgttgcg taacgcggtc gaccacggta ttgaagcgcc
     2521 atcgctacgc cgggctcttg gcaaaccagc ggaaggcacg attacgctgg aagcccgtca
     2581 tagcgcgggt tcgcttttca tcagcgtcag cgacgacggt ggcggcgtgg acctggacgc
     2641 gctgcgccgg gctatcgtcc agaaacgcct ggctagcgag accacggctg cacgtctttc
     2701 ggatgccgaa ctacttgaat tcctgttcct gcccggcttc tccatgcgcg acaccgtcac
     2761 cgacgtctcc ggccgcggcg ttggcctgga cgcggtacag gatgtcgtgc ggcaagttcg
     2821 cggcaacctg cgtgtcacgc aggaggtgct cgtcggcacg cgtttcgtga tgcagttgcc
     2881 gctgacactg tccgtgatcc gcagtctgat cgttgaagtg gatggcgagc cgtacggcct
     2941 gccgctcgcg cacgtgacac gcacgcttgt cctgccgcgg gcatcgatcg acctgctcga
     3001 agggcatcag catttcgcgt tcgaagggcg gcggcttggc attgtcaccg cgcaccagat
     3061 cctccgctca ggcaccttcg agaccgccag cgacacgctc aatgtcgtcg tgatcgggca
     3121 gggcgcggcg tgctacggcg tggtcgtcga tcgcttcctc ggcgagcgca tgctggtcgt
     3181 gcaatccctc gacaaacgcc tggggaaggt gccgaacatc gcagcgggtg cgttgatgga
     3241 aaacggtgat ccgctgctga ttgccgatct cgatgactgg ctgcgcgcgg tcgaaaagct
     3301 ggttgtcgac ggcgagcttg ggcgtgtttc atccggcacg gcgagcgtga tcgccgcgtc
     3361 gagaaaacgc gtgctggtgg ttgacgattc gctgaccgtg cgtgagctcg aacgcaagct
     3421 gctggccgga cgcggttacg acgtgaccgt ggccgtcgac ggcatggacg gctggaacgc
     3481 ggtacgcagc gagcgtttca acctggtgat cacagacatc gacatgccgc gcctcgacgg
     3541 catcgaactg gtgacgctga tccggcgcga tccgcaactg cgcgagacgc cgttgatgat
     3601 cgtgtcgtac aaggatcgcg cggaagaccg ccagcggggg ctggatgccg gagcggacta
     3661 ttatctggcg aaaggcagtt tccacgacca gaccttgctc gatgcagtgc gggatctgat
     3721 cggcgaagcg cagtcgtgag aacactaaaa aagagaggcg agcgatgaag atcgggatcg
     3781 tgaacgacat gccgctggcg gtggacgccc tgcgcgcggc gctggcgacg cggcgtgact
     3841 tcgaggtggt ctgggtcgcc cgtgacggcg cgcaggcggt cgattactgc atggctcaga
     3901 agccggacgt cgtgctgatg gatctcgtca tgccacatat ggacggcacg gaagccacgc
     3961 ggcggatcat gcgcgagacg ccgtgcgcga tcctgatcgt gaccgtggat gtgggcgcga
     4021 acgcctggcg ggtctacgag gcgatgggcg cgggcgcgct ggatgccgtc gacaccccgg
     4081 tgctcgccgg cccggacgca aagcggggga ttgccgcgct gaccgccaag atcgaccaga
     4141 ttgccgcaca gcaggccacg aaagccaccg cgaatccgat caccaccgcg ccgcgtatta
     4201 cctccgacac cgacttgctc gcgatcggtg cgtcggcggg cggtccaggc gcgctagcga
     4261 cactgctggc cgcgctgccg gccgacttcg cgccggctac ggtgattgtc cagcacgtgg
     4321 accaggcctt tgcgatcggg atggctgact ggctcaacga acagtcgacg ctgccggtgc
     4381 gcgtggcgct cgagggcgac cggccagagc cgggctgtgt gctgcttgcg gcgaccaatg
     4441 accacctgca tttccgtggc ggcggaaaca ggctcggata cacgaaagag ccgttagcga
     4501 cgccgtaccg gccgtccatc gatgtgtttt ttcaaagcgt ggtcgcgcgc tggagcggca
     4561 acgcggttgg cgtgctgctg accggcatgg gccgcgatgg cgcggcgggt ctcgccgcca
     4621 tgcgcgccaa gggttatcac acgatcgcgc aggacgaagc gacatccgcc gtgtacggca
     4681 tgccgaaagc agccgcagcg ctgaatgcgg cggtagcgat cctgccactc ccgaacattg
     4741 cgaacgccgt gcaaaaggcg ttcacatcgt cgcgcaaatg acgcgctcga actcgactca
     4801 gaacaggatt cagtcatgga acatcgaaat gcagatccgt tggcggagcg cgcccggcgc
     4861 gagcaggccg gccccgaatc ccgtccggaa agcccggcga ggcccctcga cgctacaggc
     4921 gaggagcgtt cggccgccga tctgcttgcc gatgcgctga atgcgccaag cgccgccgac
     4981 agcgcggtca tggtgctgct ggtcgacgac cagaccattg ttgccgaagc ggtgcgccgc
     5041 gcgctggtgg atcagccgcg gctggatttt cactactgcg cgaaccccga ggatgcgttg
     5101 cgcgtcgcac aggccacgcg gccgaccgtg atcctgcagg atctggtcat gccgggcgtc
     5161 gacggactga cgctggtgcg tcaataccgc gagaacgaaa tgacccgcga tattccgatc
     5221 attgtgttgt cgacgaagga agaaccgcgc atcaagagcg ccgcgtttgc cgcaggcgcc
     5281 aacgattatc tggtgaagct gccggacact attgaactga ttgcgcggat tcgataccac
     5341 tcacgctcgt atctgaattt gttgcagcgt gacgaggcgt atcgcgcgtt acgtatgtcg
     5401 cagcaaaagc tgctggagac gaacctggaa ctgcagcgcc tgaccaattc ggacgggttg
     5461 acgggtttat cgaaccgccg gtgtctcgac gattacatga acgccgagtg gaaacgcgcg
     5521 gcgcgcgaac agtcgccact tggcttcctg atgatcgatg tggacaactt caagtcgtac
     5581 aacgacacct acggccacgt agcgggtgac gacgtgctga agctcgtcgc gcgcgcgatt
     5641 gaatcgagcc tgaaccgtcc cgccgatctt gccgcccgct tcggcggcga ggaattcgct
     5701 gtcgtgctgc cgtccacgtc gactggcggc ttgcgcctgc tcggtgaaaa gatccgcatg
     5761 gcggtggagg cgcttgccgt gccgcatacg ggttcagcga gcgatcggct cacaataagc
     5821 gtgggcggtg tcatgcaggt gccgcagccg ggcgaagaca tgaagcagat gatcgaagcc
     5881 gccgacctgg ctttgtatcg ggccaagcgt gacggacgca atcgggtgga aatcgcgaat
     5941 taatcgcgaa gtaatcgcca actaatcgcc aattgagccg gtccgcgagg agcgggtcgc
     6001 ttcgccgttc accgtgcccg tggcgccttt tagcggcctt ttaccgatac cgcgcgcttg
     6061 ccgacactga acggcaccac gcacgcgtcg gcgctctggt tgcccttgcg cgcctcgttc
     6121 ccgatgatcg cgacgcgttt gaggcgtcac gaacaatcgg acgggtctgg cgatgcatcg
     6181 gccgagccgc tgccaaaact gttggcgatg cctctgcaaa tgaagcgtct cgggcgtcgc
     6241 tgacgagcgg tagccggaca tggggcgtga ccacgaaatt cgagtgcgag ctgctgtcaa
     6301 agccagtaga agaagccacc ggcgcgggcg tcacaacgtc gttcgatgca gcgcggccgg
     6361 ccaacggcgc gctccacgga tcggccggcg gagggggtgc actgtgtacc ggcgctttcg
     6421 ccggttcgcc tggtttggcg acgggtgtaa tgcgcttgag ccacgcgtca tgggacgcaa
     6481 tcagaccggg tgaacgtcca cgcggtgttt cattccagtc ggagtgatcc attgtgaggg
     6541 cagagggtgc ttccttgatg gttgttgcta tcgggtcagg cgcacgcttc gatggggctg
     6601 ttcgcggctc ttgcgctgat gcagacgctg ctgcccctgg tgatgttgat gacgcagcat
     6661 ggatttgtcc ggacgatttg aaccggtccg cggccggtgc gctccaggca tcgtcaaccg
     6721 cgagagtggg tgctgcgggg ttggtccagg cgtcatccac tacgagtttg ggcgcggcgt
     6781 gactgctcca ttgctcaccc gcggacacgc tgtcaacggc gggttctccc gcgcgggccg
     6841 ttgcgtgcat caaaccaaag gcggcgcacg ccagcgcgat tttttccagt cctgaatgaa
     6901 cgagccgaaa caggtcgcga agcggtgagc gagacatggc atttcccttg aatacacgaa
     6961 agctcgcagc ctatgggaaa gtccgatccg aaataacgcg atttatctga atagtgaaag
     7021 ttttcagcga agaagttaag aagttaactt gaggggaggg tacggccgca ttttgatggc
     7081 gctgcgatgt ttcggcgaat ccggattcgt gacattcgat gtcgttgatt cgatgtcgtt
     7141 gattcgatgt cgttgatccc aggcttctcc tggcgacgtg gacactgtcc gatagtgctc
     7201 atggagtgca aagagccgtt ccggctcata tcggaacggc tctttgcatg ccgcgctcgc
     7261 ttcggtttcc acgcgtcaga tgtgctattt cgctgcttcc gttgctttcg ccgcagccgc
     7321 aagattcgtc gcgccgccgg ccggcgaacc cattgcctgg aacagcaacg cggtatcgct
     7381 gagccgtgcg ctttcatagc gaatagcatc cagccgcgca ttgcgatact ggcgctcgct
     7441 tgcacgtgac gccgagggcg gaatcgcccc gagccgctcg cgagccgccg aatccgtgaa
     7501 cgcgctcatt gaagtgcggc tggcggtatc ggcgaaactg agtgcctggt tatcctgctc
     7561 gagcgctgcg agcgaatctg ccacgttttg gaacgcgctc agcaccgtct gcttgtattg
     7621 ggccacggcc gcctcatagc ttgcttgcgc tgcgcgcttc tgggcgagca atgcgccgcc
     7681 atgaaacagc ggctgcgaca gcgaggcgcc tatgccccag atcgcgcccg cgccggacag
     7741 ggcggtcggc cagctaaagc ccgctttccc catcgatgct gacagcgtca ggctcgggaa
     7801 catctgcgct gtcgcgacgc cgacttgcgc ggcggcggct ttgagcagcg catcggcggc
     7861 ctggatatcc gggcggctgg caagcaattc cgatggcacg accaccggca gattgcctgg
     7921 cacggtgagc gacgcgagat cgagatccgg cggcgcctga tccggcgtac ggccgatcat
     7981 gacggcaagc gcatgacgcg ttgcaagcca ctgagcccgc agcgccggca gcgaagcagc
     8041 gagcgcggcg gcgttcgatt gcgcgttgag cgcgtccgaa cgcgacgccg aaccgagttc
     8101 gtagcggcgc tgcgtttctc gcgcgtcttc attggcgagg acgatcaggc gctcggtcgt
     8161 atcgatttgc gcgcgcaacg acgacgcacc aatcgcgccc gtgacgatgt tcgcggcgag
     8221 cgcgcggcgg gcagcatcaa attgccagga ctggctgtcc acctgcgcgg cgagcgcccg
     8281 gtcagcgagt cgcgccgcac cgaacagatc gaacgtgtat tgcgcccgga cctggccgac
     8341 aaagaggttg tagagcacag tgggcggacc gaagttcggc acgccgattg cacgctgccg
     8401 gttcgcctgg ccaccgagat cgatgcttgg cagcatcgac tcgccgactt gcgcccgcag
     8461 ctgctcgcgt gcagcggcga gggttttgtc tgtcgcggcg aggctcgggc tgttgcgcaa
     8521 gccttcatcg accagcgcgt tcaacggctc cgaacggtac agcttccacc actcgggcac
     8581 gggtttcgcg ccggcatcga aactctgcga tatcccttcg gccgccaccg tgcgcgttgg
     8641 ttgtgcgtcg gcgccatagt gggccaccgt cggcatgaca ggcggttttc catccgggcc
     8701 aaacgagcac gcggaaaggg cggctgcaaa gccaagagca gtgagggcag agcgtgtcat
     8761 gactggcctc cgttgtgcgc atcgggcgtg ttcggatctt caaaccctgc gcgctcatta
     8821 cgccgcacct tgaacgaagc cgcatacaac gccggcagat aaaacagcgt aagcaccgtc
     8881 gcgctcgtga tcccgcccat caacgcggtt gccatcgggc cgaagaagtt gctgcgcaac
     8941 agcggaatca gcgcgagcac ggctgcggcc gcggtcagcg tgatcggccg gaaacgccga
     9001 acggtcgcgc caacaatggc gtcaaagcga ggatgccccg cggcaatgtc ctgctcgatc
     9061 tggtcgacga gaatcaccga gttgcgcatg atgatcccga acatggcgat cacgcccagc
     9121 atggcgacaa acccgaacgg cttgttgaac agcagcaacg cgatcacgac gccaatcaac
     9181 ccgagcggtg cggtgagcac caccatcatc acccgtgcga agctctgcaa ctggatcatc
     9241 aagagcgtca gcacgacaat tgccatgatc ggcatttgcg cgttgatcga cgactggccc
     9301 ttcgcgcttt cctcaaccgg tccgcccact tctatccgat accccaccgg caagctcgcg
     9361 cgcaacgtgg cgagtttctt ttcgatcgca tgcgtcacgt caataccttg cgcgccggcc
     9421 ttcacgtccg actgcacggt gatggtcggc tggcggtcgc gttcccagac gacgccgtat
     9481 tccaggtcat cgaaaaatgg acgagcgtcc cgagcggcac ggctccattg ggcgtc
//
LOCUS       DATASH010000221         3329 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4183204,
            whole genome shotgun sequence.
ACCESSION   DATASH010000221 DATASH010000000
VERSION     DATASH010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4183204"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /gene="rdgC"
                     /locus_tag="VHV99_06190"
     CDS             <1..265
                     /gene="rdgC"
                     /locus_tag="VHV99_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HEX3635068.1"
                     /translation="EAGKQCMRLAMTWNDRVSFVLTPSLTIKRIAPLDILKEASDPTA
                     QNDDERFDSDLTLMTAELDRMLNGLLDALGGEQGEAMPQAAAA"
     gene            413..742
                     /locus_tag="VHV99_06195"
                     /pseudo
     CDS             413..742
                     /locus_tag="VHV99_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014252153.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
     gene            1107..2000
                     /locus_tag="VHV99_06200"
     CDS             1107..2000
                     /locus_tag="VHV99_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEX3635069.1"
                     /translation="MIEPQRFTVRHVSVYRYSEPVRFGEHRMMFRPRASHDLRLVTNQ
                     LVITPTPSRLHWLHDVFDNSVAVASFVGKASELRFDSEVTLEHVETPLPDYALEPYAV
                     NWPFAYTNEEASDLVNARSRQYPDSDVDEWARRFVPVQGAGSGKNGTAGTMALLRAMT
                     LEIKNTFFYVRRAEKGVHRPGDTLRSRSGSCRDFAVLMMDAVRSLGLAARFVSGYLFV
                     PEHDATQGGGATHAWLQIYLPGAGWVDFDPTNSIVGNRHLIRVAVAWNYYQALPLWGT
                     WHGAPHSFRGLQVDVSVTAGQ"
     gene            2119..2922
                     /locus_tag="VHV99_06205"
     CDS             2119..2922
                     /locus_tag="VHV99_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEX3635070.1"
                     /translation="MKLRVGYELVYECVQPTPMMLMLNTHYSHAKEVLSPDLLAVDPP
                     VPIRQYRDSFGNLCSRIVAPQGLIALSTSAVLEVSSEPERRPPFTEQHAVEALPDDSL
                     VFLLGSRYCETDLLSEFAWQTFGKMPLGRGRVDAICDYVHQHIVFGYDFARPTKTAWQ
                     AWQEKQGVCRDFAHLAVTLCRAMNIPARYCTGYISDVGTPPPYATMDFAAWFEAYVGG
                     CWQIFDPRNNVPRTGRVLMARGRDAADVAISNAFGATQLMKFDVVCEAV"
BASE COUNT          570 a          940 c         1115 g          704 t
ORIGIN      
        1 cgaagccggc aagcaatgca tgcgtctggc gatgacgtgg aacgaccgcg tgtcgttcgt
       61 gctgacgccc tcgctcacga tcaagcggat cgcgccgctc gatatcctca aggaagcgag
      121 cgacccgacc gcgcagaacg acgatgagcg cttcgattcc gatctcacgc tgatgaccgc
      181 cgaactcgat cgcatgctga acggtctgct cgacgcgttg ggcggcgagc agggcgaggc
      241 gatgccgcaa gccgcggctg cctgagtcca gcgcaaacgc ttcgatatgc ggccgcgacc
      301 atgcggccat gcacgggtcc gcgatcccgc aagacgcgcc gactgtccat gaacgcgtcg
      361 acatgcgcgg gcacagcccc cggtacccga gcgtgcgctc cagctccgtg aactggaagc
      421 ggcgatcgaa ggggcgtcgg cgcgcgtgtg gggtcgcctt ggcggctggc ggccattttt
      481 gcgagtgatt acgacgccgg aatggtgggt ggccgcgcaa ctgcgcaaac gcgccacgac
      541 gatggcgtgg acgcccggtc tcacgctcga ctatgcccgc gcgctcagca tgctcgccga
      601 cccgcgtccg tcgggttggg tgaagatgac gcgcctcgct ttgcgcagaa cgctgcgttc
      661 gcggcagcaa tggcgcgagg aagcgcgcac ggcctcacgg gacgccgcga gcggacctgc
      721 cgcgggcgcg gtgcgtgatt gactggccgc gatccgcgcg cgacacctcg gccttgacct
      781 gcccgacttc gatctggcgg taaaaatagc aggcatatca agagcttgcc gattttgccg
      841 gatagttatg cacagccttg cgaacagatt ctgttgataa cttcgacatg cgcggcgccg
      901 ctgccaaatg agtgatttca accaatttgg cgggtgtatg ctcaaagcga aagctgctcg
      961 ttgagcaaac tcgaccacgc cgaatcctgg ccgtggctca ttcaggatca aaccgcgccg
     1021 ggcgctcgcc gcctgccgcg gcagcggttt taccagcgtg aacgcaatgc tcaggacgaa
     1081 cccagttgca cccatcggcc tgtcagatga ttgagcccca gcgtttcacg gtgcgtcacg
     1141 tcagcgtcta ccgttactcg gaacccgtgc ggttcggcga acaccggatg atgttccgcc
     1201 cgcgcgccag tcacgatttg cgcctcgtca cgaaccagtt ggtgattaca ccgacgccct
     1261 cgcggctgca ctggctgcac gacgtgttcg acaactcggt ggccgtcgcg agtttcgtgg
     1321 gcaaggcgag cgaattgcgc ttcgatagcg aagtgacact cgaacatgtc gaaacaccgt
     1381 tgcccgacta cgcgctggaa ccctacgcgg tgaactggcc gttcgcgtac accaacgagg
     1441 aggcatccga cctcgtgaat gcgcgcagcc gccagtatcc cgacagcgac gtggacgagt
     1501 gggcgcgccg cttcgtccct gtgcagggcg ccggcagcgg caagaacggc acagccggga
     1561 cgatggcgct gctgcgcgcg atgacgctcg aaatcaagaa cacgtttttc tatgtgcgcc
     1621 gcgcggaaaa gggcgtgcac cgtcctggcg acaccttgcg cagccgcagc ggcagttgcc
     1681 gcgattttgc cgtgctgatg atggacgcgg tgcgttcgct tggcctcgcg gcgcgctttg
     1741 tgagcggtta tctgtttgtg cccgagcatg acgccaccca gggcggcggc gcgacgcacg
     1801 catggctgca aatctatttg ccgggcgccg gctgggtcga tttcgatccg accaatagca
     1861 ttgtcggcaa tcgccatctg atacgggtcg cggtggcgtg gaactactac caggcgttgc
     1921 cgttgtgggg tacatggcat ggcgcgccgc attcgtttcg cggtttgcag gtcgatgtga
     1981 gcgtgaccgc ggggcagtga cggcgcgggc cgggtggggt tgaccttggt acaaatcgaa
     2041 cgtgttagct gggggcgtca actcgggttg cttaccgggc gcgtcaattg aacgtttcaa
     2101 ctggaagagg gtgtcgcgat gaaattgcgc gttggctacg agctggtcta tgagtgcgtg
     2161 caaccgacgc cgatgatgtt gatgttgaac acgcactatt cgcacgcgaa ggaagtgttg
     2221 agtccggatt tgctggcggt cgatccgcca gtgccgatca ggcagtatcg cgatagtttc
     2281 ggcaatttat gcagtcggat cgtggcgccg caggggttga tcgcgttgtc gacgagtgcc
     2341 gtgctcgagg tgtcgtccga gccggagagg cggccgccgt ttactgagca acacgcggtt
     2401 gaggcgttgc ctgatgattc gttggtgttt ctgctgggga gccgttattg cgaaacggat
     2461 ttgctgtccg aatttgcgtg gcagacgttt gggaagatgc cgctcgggcg cgggcgggtg
     2521 gatgcgattt gtgattatgt gcatcagcat attgtgtttg gatatgattt cgcgcggccg
     2581 accaagactg cctggcaggc gtggcaggag aagcagggcg tgtgccggga ttttgcgcat
     2641 ctggctgtga cgctgtgccg ggcgatgaat ataccggcgc ggtattgcac ggggtatatc
     2701 agtgatgttg ggacgccgcc gccgtatgcg actatggatt ttgctgcgtg gtttgaggct
     2761 tatgttggag ggtgttggca gatcttcgat ccgcggaata acgtgccccg gacggggcgg
     2821 gttttgatgg cccgcgggag ggatgctgct gatgttgcta ttagtaatgc gtttggggct
     2881 acgcagttga tgaagtttga tgtggtttgt gaggcggtgt agaggggggt ctttttgcct
     2941 tggcggcggt tggttttgtt tgcctgctcg gcgcttggtt gtctgtgtct ttatggcgtt
     3001 ggcctttcct tgatttgttg gtggtctatt agcgttgccc ctgtgcgggg cggcacctac
     3061 ttttctttgc cggccgcaaa gaaaagtagg caaaagaaag cggctcacac cgctagctca
     3121 taagcgggcc ccccgcgcag ttccggtagt ggtgcatctg gaatctgact tctcgcacat
     3181 tccgcgttag tgacacagcc gtcatacttc cggcagcgct gcgcgtgccg tagcggtact
     3241 ttcctaaacc cctcgccccc tttccactcg gtcgatcgac agacgcctcg aacgccttgg
     3301 cgatcctatc gtgccgcttg ccgctacgg
//
LOCUS       DATASH010000222         2153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4579060,
            whole genome shotgun sequence.
ACCESSION   DATASH010000222 DATASH010000000
VERSION     DATASH010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4579060"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..987
                     /locus_tag="VHV99_06210"
     CDS             <1..987
                     /locus_tag="VHV99_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX3635071.1"
                     /translation="TSAASLEVKRQARRAAQLESPVLLLGETGTGKELLAHAIHGGSA
                     RANQPLVTVNVAAIPDTLLEVEFFGAAPGAYTGADRKGRVGKFELANGGTLFLDEIGD
                     MPLPLQGKLLRVLQDKEFEPLGSNRIVRADVRIIAATSADLPALVAAGRFRADLFYRL
                     NVLTILAPALRERLSDIEALAYAMLEDLSTQARGASHFELQDDALRLLCSYGWPGNVR
                     ELRNTLERAVMLSDSERIDARALAPFMGPALGGGRRPHVSSALSGAHASDMAAAGAPQ
                     AASWSDAMAAFEKRFLSDALHANGGRVIETATQIGMGRATLYKKIAAYGIEM"
     gene            1074..1334
                     /locus_tag="VHV99_06215"
     CDS             1074..1334
                     /locus_tag="VHV99_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635072.1"
                     /translation="MKRSLSSFARRACAALALPAIAMLGGCSSSAPLFLPDGRPTTLV
                     QCPAGSDSCSQQASAACGGAFDVVRQSSENGTLSLIYACRAK"
     gene            1846..2049
                     /locus_tag="VHV99_06220"
     CDS             1846..2049
                     /locus_tag="VHV99_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635073.1"
                     /translation="MNHRQLEKDIEHLEHVISHISAEDRIPLSYWRNRIASVSGAALM
                     PAQASRVKRLNAALSALEARQKA"
BASE COUNT          419 a          642 c          678 g          414 t
ORIGIN      
        1 acgagcgcgg ccagtctcga agtgaagcgc caggcgcgcc gcgccgcgca actcgagtcg
       61 ccggtgctgt tgctcggcga aaccggcacg ggtaaggaac tgctggcgca tgccattcac
      121 ggcgggtcgg cgcgagcaaa tcagccgctc gtgaccgtca acgtcgcggc gattccggac
      181 acgttgctgg aagttgaatt cttcggcgcc gcgcccggcg cctatacggg cgccgaccgg
      241 aaaggacgcg tcggcaagtt cgagctcgcg aacggcggca cgctgtttct cgacgaaatc
      301 ggcgacatgc cgttgccgtt gcaaggcaag ctgctgcgcg tgctgcagga caaggaattc
      361 gagccgcttg gctcgaaccg gatcgtgcgc gcggatgtgc ggatcatcgc ggccacgtcg
      421 gccgatttgc cggcgctggt cgcggcgggg cgttttcgcg cagacctgtt ctatcggctg
      481 aacgtattga cgatcctggc gccggcgctt cgcgagcgtt tgtccgatat tgaagcgctg
      541 gcctacgcca tgctcgagga cctatcgacg caagcgcgcg gcgccagtca cttcgaattg
      601 caggacgatg ccttgcggct gctgtgttcc tacggatggc ccggcaatgt gcgcgagctg
      661 cgcaatacgc tggagcgggc ggtgatgctc tcggacagcg agcgaatcga cgcacgtgcg
      721 ttggcgccgt tcatgggtcc ggcactgggc ggcggccgtc gtccgcacgt gtcgagcgcg
      781 ctttccgggg cgcacgcttc ggatatggcg gctgcggggg cgccccaggc ggcgtcgtgg
      841 agcgacgcca tggcggcgtt cgaaaaacgc ttcctgagcg acgccttgca cgccaacggt
      901 ggacgcgtga tcgagacggc gacgcagatc ggcatgggcc gcgcgacgct ctacaagaag
      961 atcgcggcct acggcatcga gatgtgacaa catccggcga catcgagtga cgcagcgcga
     1021 ttgccgcgtg gcacaatcgc gtgatcgaaa aacaagacgt gatcggggtt tcaatgaaac
     1081 gcagtctctc ctctttcgcg cgccgggcct gtgccgcgct ggcgcttccc gcaatcgcca
     1141 tgctgggcgg ttgcagcagt agtgcaccgc tgtttctgcc ggacggccgg ccgactacgc
     1201 tggttcaatg tccggccggc tccgactcct gctcgcagca ggcaagcgcg gcgtgcggcg
     1261 gcgctttcga cgtggtgcgc caatccagcg agaacggcac gctcagcctg atttacgcct
     1321 gccgggcgaa atgaggtcgc gccagccaac ggctggcgtc gcaggcttgt cactcctttc
     1381 ctaacccgcc gcccggttga gacggcgggc gtctccttct cagcgcagat ctcatttgat
     1441 cgtggcaaac cggatatcag gcgagccgct gtcacacgcg ctgtcactca ataagcacat
     1501 ggccgtcata actatctacc gctaatgtgc atttactgaa acgctatgat gcgcattgga
     1561 tatttgccga accgctttga gattattatt cactgtttga gccgataaca aatcgccgcg
     1621 atctcgccgt tagaccgagt ccgtgcgcca cctaacggaa cgcgaagatt cttcgcagta
     1681 tcgtcatctg gccggctcga aactaaacct atacgcaaaa cttgccggct agcggcgggg
     1741 cggattgccg ctaaagaaag attttccgtt tgagagactg aatggcgtaa ttgcggcacg
     1801 tttcatgctg ttgaacgagt tcaatgagta attccgggga aaacaatgaa tcaccgccaa
     1861 ctcgaaaaag atatcgagca tcttgaacat gtcatttcgc atatctccgc tgaagatcgt
     1921 attccgctgt catattggcg caatcgcatt gcgtctgtgt ccggggcggc actgatgccc
     1981 gcgcaagcca gtcgcgtcaa acggctcaat gcagcactgt cggccctgga ggcgcggcag
     2041 aaggcctgac cgtcccacgg aaggaagatg acgtgtacct tctgatcggg taacgttctt
     2101 tgcaaaaata agggaacgta acgcctaccg actcaggagg aacatgcaac cag
//
LOCUS       DATASH010000223         6759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2007276,
            whole genome shotgun sequence.
ACCESSION   DATASH010000223 DATASH010000000
VERSION     DATASH010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6759
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2007276"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..891)
                     /locus_tag="VHV99_06225"
     CDS             complement(7..891)
                     /locus_tag="VHV99_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEX3635074.1"
                     /translation="MTSKTIISCALTGSDDTPRLSPAVPVTPEQIANEAIAACEAGAS
                     IAHIHVRDPRTGVPSTDLSLYREVVSRIRDSGCPILINLTTGPGARFVPSDEDPNRGS
                     ELSTMMTPEVRVQHVLALRPEICTLDVATMNFGNRAFVNVPDYLVKMATLIEEAGVKP
                     EIEVFDLGHVRLARHLIETQGILQAPLFQLCLGVPWGASADTESLLQMKRYLPENARW
                     SAFGISRAQFPIVAQSVILGGHVRVGLEDNLYLAKGELAPGNAALVKRAVSIVKSIGA
                     DVATPDEARSILGLPRRQ"
     gene            complement(1009..2415)
                     /locus_tag="VHV99_06230"
     CDS             complement(1009..2415)
                     /locus_tag="VHV99_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007580888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX3635075.1"
                     /translation="MPYTTINPATGELLATYDDLSDPLLEAKLAAAQHAFETDWRRRP
                     VSERGRIVSRAAALLREKADEYAGYLTLEMGKRVGEARKEVESSASILDYYAKHAHAY
                     LQPRPLAEAPGAAVHIEPIGVLFGIEPWNFPYYQIARVAGPQLMVGNVLLLKHAESVP
                     QSALAFARLFEEAGAPEGVYTNLFISIEQAGRVIDDPRVQGVTLTGSERAGAAVAERA
                     GRNLKKVVLELGGSDPLIVLEDAPLDWAIQSAVAGRMLNAGQCCVGSKRIIVVGKARG
                     DAFLEGFAKRMAALEPGDPADPATAVAPLSSERALTLLLEQIDRAASHGARIVCGGKR
                     VKCPGFYLQPTVLTDISPQNPVFTEELFGPVAAFHVVEDEAAAIALANGTPYGLGASV
                     FTADTERGERVAAQIDSGMVFINQPFGTAAELPFGGVKRSGFGRELSQLGFDEFVNKK
                     LIRVAPVGAAPFGPDRAA"
     gene            2577..2873
                     /locus_tag="VHV99_06235"
     CDS             2577..2873
                     /locus_tag="VHV99_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007580890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEX3635076.1"
                     /translation="MAKGYWVAAYRAVHDASKLAAYGVIATQVVKAAGGKFLVRGGEV
                     VAFQAGLKERTVVVEFPSFEQALATFQSEAYQKALAVLGDGAERDARVVAGVDG"
     gene            2945..3721
                     /locus_tag="VHV99_06240"
     CDS             2945..3721
                     /locus_tag="VHV99_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX3635077.1"
                     /translation="MDEAIVLSELKGHVTVLTMNRRPHNLMGPTLYRALLDEFDTAVK
                     RGARAILLRSGLRHFSAGAEVALFPDRIATQGKALMDPLEVLRGFETLPIPIVVAVNG
                     TCLGGGFEVALACDFSVVAESAKIGSVEVALGLHPLLGGIQRQVMRAGPARAKELAML
                     GRRYDAATLERWGLINLVVPDDKLDEVALSVAQELAAGPTIAHAATKQLVRVALNEGV
                     AAADEAMFELQKPIWASQDLRRGLEAFDTAGPGTAIFEGN"
     gene            3723..4988
                     /locus_tag="VHV99_06245"
     CDS             3723..4988
                     /locus_tag="VHV99_06245"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007580895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX3635078.1"
                     /translation="MAGPLEGLKVVDFSRVLAGPLCARTLADLGADVIKIEPPRPDVS
                     RAAFPNRDGMSGYYAQQNSGKRNVSIDLNAPHARELVLRLCDEADIVVENFRAGTLKG
                     FGLDYNTLSRRNPKLVYVSITGYGQRGSWASRMAYAPTVQAETGFTHNTLRHFDSAGE
                     GRRWTDPLSHADVYAGLQATIAVLAAVRKREVTGRGQYIDVAMAAVMLSINERAHLDL
                     AGIDTGAEPGILGAADGPHFVGPGGEEFVAAQSIVGSNTFPNYLRAMRRMDLARDPRF
                     STAERRLQNYSALHAVIQTWMLSFRDLKTLDAQLDEAKIAIGQIRTLKELAGTDWAKQ
                     WGAVHEIPDRRGGSYLIHGYPWHFSDDELGMRSAPAFRGEHNEQVFREVGLADVEIRN
                     AVDTGILVGGPPSATKEIDAAVLAQARSN"
     gene            5335..6048
                     /locus_tag="VHV99_06250"
     CDS             5335..6048
                     /locus_tag="VHV99_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007580899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX3635079.1"
                     /translation="MSPKPSIAKNADYHHGNLRNALIEAGRSALMEVSAQDLSLRYLA
                     RMVGVSEAAPSRHFAGIDELLATIAASGYRDLAALRVAIRDSEDTALAKAYRMMRVYV
                     EFAQRHNGLFSLMIGPRIVAHQAYPELAEESNRSFGLFSATIEALAIESGWAQSDLKL
                     VTHAAWSMEHGLATLILANRAPRADTKVAVPKLIDFVVMTLLGAITAGPDHLQSVTEQ
                     CAALQKSAVTRTARKRTKA"
     gene            6192..>6759
                     /locus_tag="VHV99_06255"
     CDS             6192..>6759
                     /locus_tag="VHV99_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HEX3635080.1"
                     /translation="MPPLRAINLLQSTEGARLHGPELDRWRRWGPYLSERQWGTVRED
                     YSEYGTAWDYFPHDHARSRMYRWGEDGIAGFGNDPLDWCVSLALWNGHDPIIKERLFG
                     LTNEQGNHGEDVKELYFYLDGTPTHSYMKMLYKYPHDAYPYQDLIDENLRRGADQPEY
                     EILDTGVFDDNRYFDVCVEYAKHTPDDIV"
BASE COUNT         1337 a         2048 c         2117 g         1257 t
ORIGIN      
        1 cccattctat tggcgacggg gaagaccgag tatgctgcga gcctcgtcag gcgttgccac
       61 gtctgcgccg atgcttttga cgatgctgac cgcacgtttg accagcgctg cgttgcccgg
      121 cgccagttcc cctttggcaa ggtaaagatt gtcctcgagg ccgacgcgga catgtccgcc
      181 aagaatcaca ctttgcgcca cgatcggaaa ttgggcgcgc gagataccga acgccgacca
      241 acgggcgttt tcgggcagat agcgcttcat ctgaagaagg ctctcggtgt cggcggacgc
      301 gccccatggc acgccaagac ataactggaa cagcggagcc tgcagaatcc cctgcgtttc
      361 gatcaggtgg cgcgcgaggc gcacgtgccc gagatcgaat acctcgattt ccggcttcac
      421 ccctgcttcc tcgatcaagg ttgccatttt gacgaggtag tcgggcacgt tgacgaatgc
      481 gcgattgccg aagttcatgg tcgctacgtc cagcgtgcag atttccggac gtaacgcgag
      541 cacatgctgg acgcggacct caggcgtcat catcgtactc aattcacttc ccctattcgg
      601 gtcttcgtcg ctgggaacga aacgtgcacc tgggcccgtc gtcagattga tcaatatcgg
      661 gcacccacta tcgcggatac gcgaaacgac ttctctgtaa agcgacagat cagtactcgg
      721 aacgccagtc ctggggtccc gtacgtgaat gtgtgcgatg ctcgcaccgg cttcacatgc
      781 agcaatggcc tcattggcga tttgctcggg cgtcacgggc acggcaggac tcagccgcgg
      841 cgtgtcgtca cttccagtga gtgcacaact gataatcgtc ttgctagtca tagtgttcgt
      901 tcaaaggaaa aagaaatgaa gtgccacaat cgcgcgcctc gctgtgtgac cagcgccggg
      961 cgcgcgtcga cggaaatgac aatgagtgga cggcgcgccg ggacacgttc aggcggcccg
     1021 gtcggggccg aacggtgccg ccccgaccgg ggcaaccctg atcagctttt tgttgacgaa
     1081 ttcgtcgaag cccaactggg aaagctctcg gccaaaacct gaacgcttga cgccaccgaa
     1141 tggcagttcc gccgccgtgc cgaacggctg attgatgaac accatgccgc tgtcgatctg
     1201 cgcggcgacc cgctcgccgc gttcggtatc cgcggtgaac accgacgcac cgagaccgta
     1261 cggcgtgccg ttggcgagcg caattgcggc ggcttcgtcc tcgacgacgt ggaaggcggc
     1321 gaccgggccg aacagttctt cggtaaagac cggattttgc ggcgagatgt cggtcagaac
     1381 agtcggctgc aaatagaagc cagggcactt gacgcgcttg ccaccacaca cgatgcgtgc
     1441 gccgtgcgaa gccgcgcgat cgatctgctc aagcagcagc gtcaacgcgc gctcggacga
     1501 aagcggggcg acggcggtcg ccggatcggc tgggtcgccc ggctcgagcg ctgccatccg
     1561 cttcgcgaag ccttcgagaa aagcgtcccc gcgagccttg ccaaccacga tgatgcgttt
     1621 ggaaccaacg cagcattgac ccgcattgag catgcggccc gccaccgcgc tttggatcgc
     1681 ccagtcgagc ggcgcgtcct cgagcacgat caacggatcg ctgccgccca gttccagcac
     1741 aaccttcttc agattgcggc cggcacgctc ggcgacggcg gcaccggcgc gctcgctgcc
     1801 cgtcagggtc acgccctgga cgcgcgggtc gtcgatgaca cggccggcct gctcgatcga
     1861 gataaacaga ttcgtatata cgccttcggg cgcgcctgcc tcctcgaaca gtcgagcgaa
     1921 ggccaacgcc gactgcggca cactttccgc atgcttgagc agcaggacgt tgcccaccat
     1981 cagttgcggc ccggctacac gcgcaatctg gtagtacgga aaattccacg gctcgatgcc
     2041 gaagagcacg ccgatcggct cgatgtgcac ggctgcgccg ggtgcttccg ctagcgggcg
     2101 cggctgcaga tacgcgtgcg catgctttgc gtagtagtcg agaatgctcg ccgacgattc
     2161 cacttccttg cgcgcttcgc cgacgcgttt gcccatttcc agcgtcagat agcccgcgta
     2221 ttcgtcggcc ttttcccgca acagcgcagc cgcgcgcgac acaatgcgcc cccgctcgct
     2281 aaccggacgg cggcgccagt cggtttcaaa agcgtgctgc gccgcagcga gcttcgcttc
     2341 gagcaatggg tcggataggt cgtcataagt ggcgagcagt tcgccggtag caggattgat
     2401 agtcgtgtac ggcatgaagt tctccacgaa aaggtggcca ttgagtcatc gcattgattc
     2461 atgttaacac cgttatctcc cccgcaggcc acatttttta gaccaggaat aaccctcatt
     2521 gatatcgatg ttaacggcgt tactatagtc acgtattttt caactgagga agcgaaatgg
     2581 caaagggata ttgggtggca gcgtatcgag cggtccatga cgcaagcaag ctcgcggcat
     2641 acggcgtcat tgcgacccag gtggtgaaag cagcaggcgg aaaattcctg gtgcgaggcg
     2701 gcgaggtcgt tgcgttccag gcgggtctca aggaacggac cgtcgtggtc gaattcccga
     2761 gtttcgaaca ggctctcgcc acgtttcaaa gcgaggctta tcagaaagcg ttggctgtac
     2821 tcggggacgg agccgaaagg gacgcgcgcg tcgtcgcggg tgtcgacggc tgagacgcgc
     2881 ccaggaggct accgaggccg ccgcccgcat gaaaacgtct gaacctgaac gaaatggagc
     2941 aagaatggac gaagcaattg ttctaagcga actcaaaggt catgtgacgg ttcttacgat
     3001 gaaccgtcgc ccacataacc tgatgggtcc gacgctgtat cgcgcgctac tcgatgaatt
     3061 cgacacggcg gtgaaacgcg gcgcgcgcgc gatcctgctg cgcagcggtc tgcggcactt
     3121 ctcggccggt gccgaagtgg cgctttttcc ggaccgcatc gccacgcaag gcaaggcact
     3181 gatggatccg cttgaggtgc ttcgtggctt cgagacacta ccgatcccga tcgtcgtcgc
     3241 agtgaacggc acatgcctcg gcggtggctt cgaagtggcg ctcgcctgcg acttcagcgt
     3301 ggtcgccgaa tcggcaaaga ttggatcagt cgaggttgcg ctcggcttgc atccgctgct
     3361 cggcggcatc cagcgccagg tgatgcgcgc cggccccgcg cgcgcaaaag agctcgcgat
     3421 gctcggccgc cgttacgacg cggcgacgct cgaacgctgg ggcctgatca atctcgttgt
     3481 gcccgacgac aaactcgatg aagtcgcgct gtcggttgcg caggaactgg cggccgggcc
     3541 gaccatcgcg catgccgcga ccaagcagct cgtgcgagtc gcgctcaacg agggggtcgc
     3601 tgccgccgac gaagcgatgt tcgaactgca aaagccgatc tgggcgtcgc aggatctcag
     3661 gcgtggactc gaggcctttg ataccgccgg cccgggtacc gcgattttcg aagggaattg
     3721 acatggccgg accactcgaa ggactcaagg tcgtcgattt ttcgcgggtg ctggcgggtc
     3781 cgctgtgcgc ccggacgctg gccgatcttg gcgccgacgt tatcaagatc gagccgccgc
     3841 ggcccgatgt gtcgcgagct gcgttcccga atcgcgacgg catgtccggc tactacgcgc
     3901 agcagaatag cggcaagcgc aacgtcagca tcgatctgaa cgcgccgcac gcacgcgagc
     3961 ttgtgctacg cctctgcgac gaggcggaca tcgtcgtcga aaattttcgg gccggcacgc
     4021 tcaaggggtt cggactcgac tacaacacgc tctcccggcg caatccaaaa ctggtctacg
     4081 tgtcgatcac aggctatggt cagcgcggca gctgggcgtc gcgcatggca tacgcgccga
     4141 ctgtgcaggc cgaaacggga ttcacgcaca acacgctgcg ccatttcgac agcgcgggcg
     4201 aaggccgcag gtggaccgat cccttgtcgc acgcggacgt ctacgcgggc ctgcaggcga
     4261 caatcgccgt gctcgcggcg gtgcgcaaac gtgaagtaac cggacgcggt caatacatcg
     4321 acgttgcaat ggcggccgtg atgctgtcga tcaacgaacg cgctcacctt gatctcgcgg
     4381 gcatcgatac gggcgccgag cctggcatcc tcggtgccgc cgacggcccg catttcgttg
     4441 gccccggcgg cgaagagttc gtggcggcgc aaagcatcgt gggcagcaat acgtttccga
     4501 actatttgcg cgccatgcgc cgtatggacc ttgcacggga tccgcgcttc agcaccgctg
     4561 agcggcgcct gcagaactac agcgcgctgc atgcagtgat ccagacgtgg atgctgtcgt
     4621 tccgcgatct caagacgctc gatgcgcaac tcgacgaggc caagatcgcg ataggccaga
     4681 tccgtacgct gaaggaactg gccgggaccg actgggcaaa acagtggggt gcggtgcacg
     4741 agatcccgga tcgccgcggc ggctcgtatc tcatccatgg ctatccgtgg cacttttccg
     4801 acgacgagct tggaatgcgc agcgcgccgg cgtttcgcgg cgaacacaac gagcaggtgt
     4861 tccgcgaagt gggcctggcg gatgtcgaaa tccgcaatgc agtcgacacc ggcattctgg
     4921 tcggcggccc gccatcggca acgaaggaga tcgacgccgc tgttcttgcg caagcacggt
     4981 ccaactgaac gcaacacagc ccgttagcgc gccgcgacag cagcgcattg tcagcaatgc
     5041 gatcgcgctt tggcgataga tcggttcaaa gcgcaacgtg actagtcgat gccgacgtgg
     5101 gcgcggcggg tgctcgtggc aagtgcccgc gttgtgcact gaaaagccgc tgcgggcact
     5161 gcgcaccgct gagcggccag gcatgcgatg tatcccgacg cccagccgcg caactgagcc
     5221 gctagaatgc gtatagcgac acacgcctgt ttagcgcgtg acccgcgata ttgcgtgcct
     5281 ctccgcgcag aatttataat catttccttg ttttgctcct cgaacgaagg tcacgtgagt
     5341 cccaagccat ctatcgcgaa gaatgcggat taccaccatg gcaatctgcg caacgcgctc
     5401 atcgaagcgg ggcgcagcgc gctcatggag gtatcggcac aagacttgag tctgcgttac
     5461 ctcgcgcgca tggtgggcgt atcggaagcc gcgccctcgc ggcattttgc gggtatcgac
     5521 gaactgctgg ccacgatcgc ggcaagcggt tatcgtgacc tcgctgcgct gcgcgttgcg
     5581 atccgtgaca gtgaggatac ggcgcttgcc aaagcgtacc ggatgatgcg ggtgtatgtt
     5641 gagttcgccc agcgccacaa tgggctgttc agtctgatga tcgggcctcg catcgtggcg
     5701 catcaagcgt atccagaact cgccgaggaa agtaacaggt cttttgggtt gttttccgcg
     5761 actatcgagg cgctggctat cgagagtggt tgggctcagt ccgatctcaa gctcgtgacg
     5821 catgccgcgt ggagtatgga acatggtctc gccacgctga tcctggcgaa ccgcgcgccg
     5881 cgcgcggaca ccaaagtggc ggtgccaaag ctgatcgatt tcgtcgtgat gacgttgctt
     5941 ggcgcgataa ccgcgggtcc ggatcatctg cagagcgtca ccgaacaatg cgcggctttg
     6001 cagaagtctg cggttacgcg aacggcacgt aagcgcacca aagcctagcc atcatcaacg
     6061 tggagcaacg aggcgctgca gtgcgatggc gcttatcatt acggtacagt ctggccgccg
     6121 ttgctgatga aaaaggacag actttcatct actgaacacg ctggacggtc gatgtcgggt
     6181 cgggaggttc catgccaccg ctacgcgcaa tcaatcttct tcagtcgacg gaaggtgccc
     6241 ggctccatgg cccggagctc gaccgctggc gccgctgggg gccttatctg agcgagcggc
     6301 aatggggcac tgttcgcgaa gactacagcg aatacggcac cgcctgggac tactttccgc
     6361 acgatcatgc gcgcagccgc atgtaccgct ggggtgagga cggcattgcc ggattcggca
     6421 acgatccgct cgactggtgc gtatcgctcg cgctctggaa cgggcacgat ccgatcatca
     6481 aggaacggct cttcgggttg acgaatgagc agggcaatca cggcgaggac gtgaaggagc
     6541 tgtacttcta tctcgacggc acgccgacgc attcgtacat gaagatgctg tacaagtatc
     6601 ctcacgacgc gtacccgtac caggatctga tcgatgaaaa tctgcggcgc ggcgccgatc
     6661 agcccgaata tgaaattctc gatacgggcg tgttcgacga taaccgctac ttcgacgtct
     6721 gcgtcgagta tgcgaagcac acacccgacg atatcgtga
//
LOCUS       DATASH010000224         4106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1809311,
            whole genome shotgun sequence.
ACCESSION   DATASH010000224 DATASH010000000
VERSION     DATASH010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1809311"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1434
                     /gene="ubiB"
                     /locus_tag="VHV99_06260"
     CDS             <1..1434
                     /gene="ubiB"
                     /locus_tag="VHV99_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HEX3635081.1"
                     /translation="GVRLRLALESLGPIFVKFGQVLSTRRDLLPVDIANELAKLQDQV
                     PPFDSAVAIGLVEKSLGAPVDVLFDDFERVPVASASIAQVHFAKVKAGQHAGKAVAVK
                     VLRPNMLPVIDSDLALLRDIAVWAERLWADGKRLKPREVVAEFDKYLHDELDLMREAA
                     NGSQLRRNFAGLDLLLVPEMYWEFCTPTVLVMERMVGVPISQVETLRAAGVDIPKLAR
                     EGVEIFFTQVFRDGFFHADMHPGNIQVSLDPAHFGRYIALDFGIIGALSDFDKNYLAQ
                     NFLAFFKRDYHRVATLHLESGWVPPTTRVEELESAIRAVCEPYFDRALKDISLGQVLM
                     RLFSTSRRFNVEIQPQLVLLQKTMLNVEGLGRSLDPELDLWKTAKPYLERWMNEQIGL
                     RGWYERLKIEAPQWSKTLPQLPRLIHHVLAERHDNKRGANDDMIRQILLEQKRTNRLL
                     QGLLLFGAAVGVGAVLARAFLAFVYGG"
     gene            1464..2087
                     /locus_tag="VHV99_06265"
     CDS             1464..2087
                     /locus_tag="VHV99_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX3635082.1"
                     /translation="MSDPTQSSAPDFATRDPNSPEFWDERFARRFMPWDQAGVPAAFQ
                     SFVSRHSGAAVLIPGCGSAYEARWLAGQGNPVRAIDFSPAAVAAAREQLGAQHAQLVE
                     LADFFTYEPPFTPAWIYERAFLCALPLARRADYAQRMAELLPGGALLAGFYFIGATPK
                     GPPFGIERAELEALLTPHFDLIEDEAVNDSIAVFAGRERWMTWRRRD"
     gene            2348..2752
                     /locus_tag="VHV99_06270"
     CDS             2348..2752
                     /locus_tag="VHV99_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEX3635083.1"
                     /translation="MPIYAYRCESCGFGKDVLQKMSDPQLTQCPECAKDSFRKQVTAA
                     GFQLKGSGWYVTDFRGGNSGAGAPAKPDANGGSNANSGENAAAGNNGAAKSDTASANG
                     TAAAASSGAAATPAAPAAAPAASASSSGSGSA"
     gene            2828..3481
                     /locus_tag="VHV99_06275"
     CDS             2828..3481
                     /locus_tag="VHV99_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEX3635084.1"
                     /translation="MTTKKTTLKSVFLTGLLVLVPLAITLWVLGLIIGTMDQTLLLLP
                     SAWQPERLFGFRLPGLGAVLTLAFIFVVGLLTQNFIGQKLVKWWEVVVAHIPVVGPIY
                     TSVKQVSDTLLSSSGNAFRKALLIEYPRRGSYTIAFLTGIPGGDVINHLKEDHVSVYV
                     PTTPNPTSGFFLMVPRSEVIELDMTVDAALKYIVSMGVVAPSAPPAPVRRTTVEPPL"
     gene            3558..>4106
                     /locus_tag="VHV99_06280"
     CDS             3558..>4106
                     /locus_tag="VHV99_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006024571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEX3635085.1"
                     /translation="MSMRSEYCGLVTEELLGQSVSLCGWVSRRRDHGGVIFIDLRDRE
                     GLVQVVCDPDRAEMFKTAEGVRNEFCLQIKGVVRARPEGTTNASLKSGKIEVLCHELI
                     VLNPSITPPFQLDDDNLSETTRLTHRVLDLRRPQMQHNLRLRYRVAIEVRKYLDSLGF
                     IDIETPMLTKSTPEGARDYLVPS"
BASE COUNT          716 a         1263 c         1342 g          785 t
ORIGIN      
        1 ggcgtgcgtc tgcgtctggc gctggaaagc ctcgggccga tctttgtcaa attcggacag
       61 gtgttgtcca cgcggcgcga tctgctgccg gtggatatcg ccaatgaact ggcgaagctc
      121 caggatcagg tgccgccgtt cgactccgcg gtggcgatcg ggctggtcga gaagtcgctc
      181 ggcgcgccgg tcgacgtgct gttcgacgac ttcgaacggg tgccggtggc gagcgcgtcg
      241 atcgcgcagg tgcacttcgc gaaggtgaag gccggtcagc atgcgggcaa ggccgtcgcc
      301 gtcaaggtgc tacgtccgaa catgctgccg gtgatcgatt ccgacctcgc cttgctgcgc
      361 gacatcgccg tctgggccga gcggctgtgg gccgacggca agcgcctgaa gccgcgcgag
      421 gtggtcgccg aattcgacaa gtatctgcac gacgaactcg atctgatgcg cgaagcggcc
      481 aacggcagcc agctgcgccg caacttcgcc gggctcgatc tgctgctcgt gccggaaatg
      541 tactgggagt tctgcacgcc cacggtgctg gtgatggagc gcatggtcgg cgtgccgatc
      601 agccaggtgg agacgctgcg cgcggcgggt gtcgacattc cgaagctggc gcgtgaaggc
      661 gtcgagattt tcttcaccca ggtgtttcgc gatggttttt tccacgccga catgcacccg
      721 ggcaacattc aggtgagcct cgatccggcg cacttcggcc gctatatcgc gctggatttc
      781 ggcatcatcg gcgcgctgtc ggacttcgat aaaaactacc tcgcgcagaa cttcctcgct
      841 ttcttcaagc gggactatca tcgcgtcgcc acgctgcatc tggagtcggg ctgggtgccg
      901 cccactaccc gcgtcgaaga actggaaagc gccatccgcg cggtgtgcga gccgtatttc
      961 gaccgcgcgt tgaaggatat ttccctcggt caggtgctga tgcggctgtt ctcgacctcg
     1021 cgccgtttta acgtcgagat ccagccgcaa ctggtgctgc tacaaaaaac catgctgaat
     1081 gtggaagggc tcggccgctc gctcgatcct gaactggact tgtggaagac cgccaagccc
     1141 tatctggaac gttggatgaa cgagcagatc ggtctgcgcg gctggtacga gcggctgaag
     1201 atcgaggcgc cgcagtggag caagacgctg ccacagttgc cgcgcctgat tcatcacgtg
     1261 ctggctgaac gccacgacaa caagcgcggc gcgaatgacg acatgattcg ccagattctg
     1321 ctcgaacaga agcgcaccaa ccggttgctg caagggctgc tgctgttcgg cgcggcggtc
     1381 ggtgtgggcg cggtgctggc gcgggcgttt ctggcgttcg tctacggcgg ctgaacccga
     1441 tcctgatccc cacgaggcat gtcatgagcg acccgaccca gtcttccgca cccgactttg
     1501 cgacgcgcga ccccaactcg cctgagttct gggacgaacg cttcgcgcgc cgtttcatgc
     1561 catgggatca ggccggcgtg ccggcggcgt tccagtcgtt cgtcagccgg cacagcggcg
     1621 ctgccgtgtt gattcctggt tgtgggagtg cctacgaggc gcggtggctg gccgggcaag
     1681 gcaatcccgt ccgggcgatc gatttctcac cggccgccgt ggcggccgcg cgcgagcaat
     1741 tgggcgcgca gcatgcgcaa ctggtcgagc tggcggactt cttcacttac gagccgccat
     1801 tcacgccggc ctggatttat gagcgcgcgt tcctttgcgc gctgccgttg gcgcgccgtg
     1861 cggactacgc gcaacggatg gccgagttgt tgcctggcgg cgcgttgctg gcgggttttt
     1921 atttcatcgg cgcgacgccg aaagggccgc cgtttggcat cgagcgcgct gaactcgagg
     1981 cgttgctcac gccgcacttc gatctgatcg aagatgaagc cgtgaacgat tcgattgccg
     2041 tcttcgccgg acgcgagcgt tggatgacct ggcgccgtcg cgattgatcg caacgccgcc
     2101 gcgtgcgccg gtggccgcga tgttccgcca gctgatcggg tttgaggcgc gagccatgac
     2161 gaggacgaat cagcgtcaac atccagtggc ggcggcttaa ggcgcacttg attcgagaac
     2221 ggccgcccca atttacggga acggcggcgc gcttggcgct ggtccccgaa attcgcgggt
     2281 cgctagccat atacggctat aattcaaggc tttgcaggcg ttacaagatt tcagtaggga
     2341 aaagattatg ccgatctacg cttatcgttg cgagtcatgc ggcttcggga aggacgtgct
     2401 tcagaagatg agcgaccccc agttgaccca gtgtcccgag tgcgcgaaag acagctttcg
     2461 caagcaggtg actgcagccg gtttccagtt gaagggctcc ggctggtatg tgaccgattt
     2521 ccgcggcggc aacagcggcg cgggcgcgcc ggccaagccc gacgcgaatg gcggctcgaa
     2581 tgcgaattcg ggcgagaacg ccgcagccgg gaataacggc gcagcgaaat ccgacacggc
     2641 ttcggccaac ggcacggccg ccgccgcttc atctggcgcc gccgcaacgc ccgccgcgcc
     2701 tgctgccgca cccgccgcat cggcgagttc gagcggttcc ggcagcgcct agtgggactg
     2761 ccgccctcag gggcggcgca cacgccgcgc gtcgagtatc ggttcgcgcg gctttctggc
     2821 ggtacacatg acgacgaaaa aaacgacgct caaatcggtg ttcctgacgg gcctgctggt
     2881 gctggtgcct ctggctatca cactgtgggt gctcggcctg atcatcggca cgatggacca
     2941 gacgctgttg ctgctgccga gcgcatggca accggaacgc ctgttcggct tccgtttgcc
     3001 aggacttggc gcggtgctga cgctggcgtt catctttgtg gtcgggctgt tgacgcaaaa
     3061 cttcatcggg cagaagctcg tgaagtggtg ggaagtcgtg gttgctcaca ttccggtggt
     3121 cggcccgatc tacaccagcg tgaagcaggt gtcggacacc ttgttgtcga gcagcggcaa
     3181 tgcgtttcgc aaggcactcc tgatcgagta cccgcgccgc ggttcctata cgattgcgtt
     3241 tctgaccggc attccgggcg gcgacgtgat caaccacctg aaagaagatc acgtcagcgt
     3301 gtatgtgccg actacgccga acccaacgtc cggcttcttc ctgatggtgc ccaggagcga
     3361 agtgatcgaa ctcgacatga cggtcgacgc tgcgctcaag tacatcgtct cgatgggcgt
     3421 cgtggcgccg tccgcgccgc cggcgccggt gcgccgcacc acagtcgagc ctccgctgta
     3481 atgccggtgc gcagcgtttc acgcagtgtt tgattgctgc gcgccgttca accaatgcaa
     3541 aacgaaagac aaacatcatg tcgatgagat ctgaatactg cggtctggtg accgaagaac
     3601 tgctgggcca atccgtctcg ctgtgcggct gggtaagccg ccgtcgcgac catggcggcg
     3661 tcatcttcat cgacttgcgc gaccgcgaag gcctcgttca ggtcgtctgc gatccggatc
     3721 gcgcggagat gttcaagacg gccgaaggcg tgcgcaacga gttctgcctg cagatcaagg
     3781 gcgtggttcg cgcccgtccg gaaggcacga cgaacgcctc gctgaagagc ggcaagatcg
     3841 aagtgctgtg ccacgagctg atcgtgctga atccgtcgat cacgccgccg ttccagctcg
     3901 acgacgacaa cctgtcggaa accacgcgtc tcacgcaccg tgtgctcgac ctgcgccgtc
     3961 cgcaaatgca gcacaacctg cgtctgcgct accgcgtcgc gatcgaagtg cgcaagtacc
     4021 tggactcgct gggcttcatc gacatcgaaa cgccgatgct caccaagagc acgccggaag
     4081 gcgcgcgcga ctacctcgtg ccgtcg
//
LOCUS       DATASH010000225         9138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1612422,
            whole genome shotgun sequence.
ACCESSION   DATASH010000225 DATASH010000000
VERSION     DATASH010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 9138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9138
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1612422"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VHV99_06285"
     CDS             <1..218
                     /locus_tag="VHV99_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013691534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX3635086.1"
                     /translation="APELLAPTRRNNPKMMYWVIKNGVKMTAMPAFGKTQDDQTIWDL
                     TAFLYKGRGISAQDFDKQSAAKPNTRP"
     gene            390..661
                     /locus_tag="VHV99_06290"
                     /pseudo
     CDS             390..661
                     /locus_tag="VHV99_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010089453.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase"
     gene            1103..1867
                     /locus_tag="VHV99_06295"
     CDS             1103..1867
                     /locus_tag="VHV99_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX3635087.1"
                     /translation="MSTKNELHDSGDAPAAAPPQPSRRRFLQSAAAAATVGAAPHLRA
                     QTQAPTAPPSAEVRAPVPGRPVTLTVNGRAYTLQLEARVTLLDALREYAGLMGTKKGC
                     DRGQCGACTVIADGRRINSCLTLAVMHEGENLTTVEGLASNGVLSPMQRAFIEHDAFQ
                     CGYCTPGQLCSATALLNEFRNGTASTVTADVRSRPPQLSDDEIRERMSGNICRCGAYA
                     NIVAAVRAVHEGGGQSSASDNSSNSSSGTSRNDANA"
     gene            1881..2882
                     /locus_tag="VHV99_06300"
     CDS             1881..2882
                     /locus_tag="VHV99_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX3635088.1"
                     /translation="MDAISYERAGDVAGAVRAAQQPGAVFIGGGTNLLDLMKGGVARP
                     LRLIDITHISGLDTVTTLPDGGIRIGALVRNSDAANHALVREQYPLLSQAFLAGASSQ
                     LRNMATVGGNLLQRTRCGYFYDTAFTQCNKRMPGSGCAALDGHNRTHAILGTSPQCIA
                     VNPSDMSVALAALDAVVRVNGPAGERTIAFADFHRLPGDRPDVDTTLQAGELITAVDL
                     PPPLFSANAHYLKVRDRASYAFALVSVAAALQMDGERVTTARIALGGVAHKPWRASAA
                     EQMLNGQPLTQARLHDAAAAALRDARPQHDNRFKVQLAQRAIVRAVNQAAGRSGGVV"
     gene            2879..5101
                     /locus_tag="VHV99_06305"
     CDS             2879..5101
                     /locus_tag="VHV99_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX3635089.1"
                     /translation="MNLIGQPLDRIDGLLKVTGEARYAAEFPEARLAHAVLVTSTIAR
                     GTIASIDASRAQALPGVLLVMTYQNAPRLPNGGKSPLAPPAGRRLSLLQDNQVHYNNE
                     PVAVVVADTLEHASDAARQLRITYQSTAATLDFAQAKPNGHAPDKPQGRTTDTQRGDF
                     DDGMRSGTVHVDAVYTTPIEHHNPMEPHATMARWDGPQLILYDSTQGVSGAALAVAKT
                     LGISPGDVRVISPFIGGGFGCKGSSWSHVSLCAMAAKQTGRPVRLALERPQMFGPVGS
                     RPHTEQHFTLAARHDGTLTAMRHDSTSHTSMIEDWTETCCMVTRMLYTVPNQVTTHRI
                     VPMNLGTPTFMRAPGETTGSFALESAMDELAVALKMDPLALRLKNYADADPQENKPWS
                     GKSLRECYQIGAEKFGWSRRTGTPRSMRNGNTLIGMGMATATYPANRSEASAIARILP
                     DGSAMVASGTQDLGTGTYTVMTQVAADALGFAPENIHFALGDSSLPKAPGSGGSQSAA
                     SVSPAVRDASSQVRNQLIALALADEASPVHGIALADITVDNGWVMSRSQPAKRDPAAA
                     IIARSGGKPIEATSTVKPGDEKQQYSFHSFGAVFVEVHVDADLGTIRVARIVGVYDIG
                     RVLNEKTAHSQLMGGMVWGVGAALQEETLLDTRYGRFTNANLAEYHVPVNADIRSLDI
                     TFIDVPDPHINSLGVRGIGEIGITGVPAAIANAVYHATGVRVRDLPVTLDKVMGVMQV
                     "
     gene            5171..5758
                     /locus_tag="VHV99_06310"
     CDS             5171..5758
                     /locus_tag="VHV99_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX3635090.1"
                     /translation="MAYASLATGILLAAGLGSRFDPDGLQNKLLARMPDGTPVAQEAA
                     HRLLKVVTRVLAVVRPGAETLARLLNDAGCDVVFAATAERGMGASLAVGIEASDDAEG
                     WIVALADMPRIATSTIEAVAQALNGGASIVAPFYQGQRGHPVGFGIEHRDALMALDGD
                     TGARALLMSQRVARLDVDDPGILRDVDTPEDLRNI"
     gene            5947..7509
                     /locus_tag="VHV99_06315"
     CDS             5947..7509
                     /locus_tag="VHV99_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427835.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HEX3635091.1"
                     /translation="MKRISIEPSFAVWRRAARVLLRQGVEPSQIEWVESGSEAAVASP
                     GAGHDAGGGHAVQSSATTVVERLASNLSSSSASAVGEPDAESTRTAADSLATSTAGVL
                     AIPRELLSWLRTAACFRAPDRWSLVYRVLWRWTHGERKVLDLDDPDGALLDQRIHTVE
                     HETDDLLTLTLFRRRDPSMGPPEFVGWYEPHHDLLERAAARFAARMGESTWMLATPHG
                     AVFWNGMLLRIGRPLVEEREQTSQALTASAMTGEAVTSTPTEALWLAYYASAFNAQPS
                     PVPLRYWRMPPAGPPLPAHLARKRSRLGAQSTASTVPPTPPVEYSAMTPPLREPTGPL
                     ATCRRCALWRNAKQAVAGAGPAHAAIMVLGEQPGEHENQHGEPFTGPAGQLLDAVLAR
                     AGLERQALYLTYAVKHYKWETLDGARVHRTPVRREVEACQYWLEKELTRIAPRVVVTL
                     GATALRALTGAHVNLSEYLGQTIAHEGRLIVPAWHPSYALGVVDATLREDVVAAIVTA
                     FSRAAALAAEGA"
     gene            7823..8527
                     /locus_tag="VHV99_06320"
     CDS             7823..8527
                     /locus_tag="VHV99_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX3635092.1"
                     /translation="MSETTPRLFIVSPHFDDAVFSCGALLAAHPDVAVCTVFAAPPEQ
                     EMYTEWDEKSGFTSAHQAIRARTLEDNLALEVLDAIPLRMPFRDGQYFDSPSISKLAA
                     ALEETIYRTTANTLLMPLGLHHDDHALVFEACCEILPRLSHLTLFAYEDAIHRRTPGV
                     VQARLADLAQRGVVATPAYPSAGYTIDPPRRAQLKWEAVNAYASQLRAFGAGNYDDIF
                     AAERYWQLSVGRRAKK"
     gene            8593..8745
                     /locus_tag="VHV99_06325"
     CDS             8593..8745
                     /locus_tag="VHV99_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635093.1"
                     /translation="MSYDMHTSPIPDPNADPNRDPEADPLVPPSPGHHTEEPQRPDGP
                     PDKDPV"
     gene            complement(8875..>9138)
                     /locus_tag="VHV99_06330"
     CDS             complement(8875..>9138)
                     /locus_tag="VHV99_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011658556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate aminotransferase"
                     /protein_id="HEX3635094.1"
                     /translation="EQARAGLAERGIGSVLAPEQSSVVLRAYRLPEGVAYPRLHDALK
                     ARGFVIYAGQGGLSAELFRISTMGNIHATDIERLLQGFTELTR"
BASE COUNT         1659 a         3085 c         2881 g         1513 t
ORIGIN      
        1 tcgcgccgga actgctcgca ccaacacgcc gcaacaatcc caagatgatg tactgggtaa
       61 tcaagaacgg cgtgaaaatg accgcgatgc ccgcgttcgg caagacgcaa gacgaccaga
      121 cgatctggga cttgacggcg ttcctctata aaggacgcgg catttccgct caggatttcg
      181 acaagcagag cgcggctaaa ccgaacaccc ggccgtaggc tacgctcgtt gagcgggtgg
      241 gaaagggtcg cttggcggcg gacatgcctt ttgcgcatcg gggtcttcag gtgctgcgcg
      301 gatgcactta taggctcggc ccctcaactt gagcggccgc atccttcacg agcgcgctct
      361 gcgccccttc gttgctaccg gctttgctct acggcgtgca tcgcgccagg ctgccgcccg
      421 gcgacatggt gggctctgcc ccgcatcgag cctacatcac gtgagtccgg tgatgcgtgg
      481 cgtgcggctc gcgtccttct tctggatcca gagcatggtg cgcgacgacg gcgaacgcgc
      541 cacgctgttc caacccgaca acgacctgca acgtctggcc gctgaacaag gctcgaacga
      601 tgcaagtgtg gtaagtctca ccggcattta tcacaatctg ctgcgccgct gggccgacgc
      661 ccgatcgagg ggactcctcg tcccacgcaa aacagtcttt ctttttgcac taagaatgaa
      721 gacgccttca cgtatttact ttcctgttcg ttaagcgcaa cgtccatgct tgacgcgcgc
      781 tgcggcaatt agttgcgctc tccacgattc gatcccgttc accgcgattc actgttggct
      841 tacggctggc tcacggatga ttcgggtgcg ccgcgtcgac atgccgttgc gttcgcaacg
      901 gcaccgaaat tgctgactga tgagttcgac cgcgaggctc tggatatacc aaagcatcgg
      961 ctatccgcat gttggatacc cgtacgtcgg aacccgcacg tcggaacccg cacgtcggat
     1021 acccgcacgt cggatacccg cacgccggat acccgtatat cgcaagcctg cattgacctt
     1081 tcaatggagc gagcagaccg ccatgtccac gaagaacgaa cttcacgatt cgggcgacgc
     1141 gcccgccgcc gcgccacccc aaccatcgcg ccgccgcttt ctgcaatcgg ctgccgctgc
     1201 cgccacggtc ggcgcggcgc cgcatctgcg cgcccaaacg caagccccca ccgcaccgcc
     1261 ttccgccgaa gttcgcgcac ccgtgccagg ccgcccggtc acactgaccg tcaacggtcg
     1321 cgcttatacg ctgcaactcg aagcgcgcgt gaccttgctc gatgcgctgc gcgagtacgc
     1381 gggcctgatg ggtacgaaga aaggctgcga ccgcgggcaa tgcggtgcat gcacggtgat
     1441 cgccgacggc cgtcgcatca attcctgcct gacgctcgcc gtaatgcacg aaggcgagaa
     1501 cctcacgacg gttgaaggac tcgccagcaa cggcgtgctg agcccgatgc aacgggcctt
     1561 catcgaacac gatgcgttcc agtgcggcta ctgtacgccc gggcagttgt gttccgcgac
     1621 tgccctcctg aacgaattcc gcaacggcac ggccagcacc gtgaccgccg atgtccgcag
     1681 ccgcccgccg caactttccg acgacgagat ccgcgaacgc atgagcggca acatctgccg
     1741 ctgcggcgcg tacgcgaata tcgtcgcggc ggtgcgcgcc gtgcatgagg gcggcgggca
     1801 aagcagcgca agcgataaca gtagcaacag cagcagcggc accagccgca acgacgccaa
     1861 cgcctgacgg agcgaccagc atggatgcga tctcttacga acgcgccggc gatgtcgccg
     1921 gcgccgtgcg cgcggcgcag caaccgggcg cggtgttcat cggcggcggc accaatctgc
     1981 tcgacttgat gaagggcggc gtggcgcggc cgctgcggct catcgatatc acgcacatca
     2041 gcgggctcga tacggtcacc acactgccgg atggcggcat ccgtatcggc gccctggtgc
     2101 gcaacagcga cgcggccaat cacgcgctgg tgcgcgaaca atacccgttg ctgtcgcaag
     2161 catttctggc gggtgcatcg tcgcaactgc gcaatatggc gacggtgggc ggcaatctgt
     2221 tgcaacgcac ccgctgcggc tacttttacg acaccgcctt cacgcagtgc aataagcgca
     2281 tgcccggcag cggctgcgcg gcgctcgacg gccacaatcg cacgcacgcg attctcggta
     2341 cgagtccgca atgcattgcc gtgaatccat cggatatgag cgtggcgctt gccgcgctcg
     2401 acgccgtggt gcgcgtcaat ggccccgcag gcgagcggac gattgcgttt gctgattttc
     2461 atcggctccc gggtgaccgg cccgacgtcg atacgacatt gcaagcgggc gaattgatta
     2521 ccgcggtcga cctgccgcca ccgctgttca gcgcgaacgc ccactatctg aaagtgcgtg
     2581 accgggccag ttacgcattc gcgctggtct cggtggccgc cgccttgcag atggacggcg
     2641 agcgcgtgac aaccgcgcgc atcgcgctgg gcggcgtcgc gcacaagccg tggcgtgcga
     2701 gcgccgccga gcagatgctc aacggccagc cgctcacgca ggcaaggctg cacgacgccg
     2761 ctgccgccgc gctacgcgat gcgaggccgc agcatgacaa tcgtttcaag gtgcagctcg
     2821 cgcaacgcgc gatcgtgcgc gcagtgaacc aggccgcggg ccgcagcgga ggtgtcgtat
     2881 gaatctgatc ggtcaaccgc tcgaccgcat cgacggtctg ctgaaagtca ccggcgaagc
     2941 gcgctacgcc gccgagttcc ccgaggcgcg gctcgcgcat gcggtacttg tcaccagcac
     3001 cattgcacgc ggcacgattg cgtcgatcga tgcgagccgt gctcaagcgc tgcccggtgt
     3061 gttgctggtg atgacgtatc agaacgcgcc acgtctgccg aacggcggca aatccccatt
     3121 ggcgccgccg gccggccgcc gcctgtcgct gctgcaggac aaccaggtcc actacaacaa
     3181 cgagccggtc gcggtggtgg tcgccgatac gctggaacat gcaagcgacg cggcgcgcca
     3241 gctgcgcatc acctatcaga gcacggccgc gacgctcgac ttcgcgcagg ccaagccgaa
     3301 cggacatgcg cctgacaaac cgcaaggccg caccaccgac acgcagcgcg gcgatttcga
     3361 cgacggcatg cgcagcggca cggtccacgt cgacgccgtc tacaccacgc cgatcgagca
     3421 tcacaacccg atggagccgc acgccacgat ggcgcgttgg gacggcccgc aactcatcct
     3481 ctacgactcc acgcaaggcg tcagcggtgc ggcgctggcg gtcgccaaaa cactcggcat
     3541 ctcccccggc gacgtgcgcg tgatttcgcc gtttatcggc ggtggtttcg gttgcaaggg
     3601 ctcgtcatgg tcgcatgtgt cgctgtgcgc gatggcggcg aaacagaccg ggcgtccagt
     3661 gcgcctcgcg ctggaacggc cgcagatgtt cgggccggtc ggctcgcgtc cgcataccga
     3721 gcagcatttc acccttgccg cgcggcacga cggcacgctg accgcgatgc gtcacgacag
     3781 cacctcgcat acgtcgatga tcgaagactg gaccgagacc tgctgcatgg tcacgcgcat
     3841 gctgtacacg gtgcccaatc aggtcaccac acaccggatc gtgccgatga atctcggcac
     3901 gccgaccttc atgcgcgcgc ccggtgaaac gaccggctcg ttcgcgctcg aatcggcgat
     3961 ggacgaactc gcggtggcgc tgaagatgga cccgctcgcg ctacgtctca agaactacgc
     4021 cgatgccgat ccgcaggaga acaaaccgtg gtcgggcaaa tccttgcgcg agtgctatca
     4081 gatcggcgcg gagaaattcg gctggtcgcg gcgcaccggc acgccgcgtt cgatgcgcaa
     4141 cggcaacacg ctgatcggca tgggcatggc gaccgcgacc tatccggcca accgcagcga
     4201 agcctcggcc atcgcgcgga ttctgccgga cggcagcgcc atggtcgcct ccggcaccca
     4261 ggacctcggc accggcacct atacggtgat gactcaggtc gccgccgacg ccctcggctt
     4321 cgcgccggaa aacatccact tcgcgctcgg cgattcgtcg ctgccgaaag cgccgggttc
     4381 gggcggctcg caatcggcgg cgagcgtctc acccgccgtg cgcgatgcct cgagccaggt
     4441 gcgcaaccag ttgatcgcgt tggcgctggc cgatgaagcg tcgcccgtgc acggcatcgc
     4501 actcgcggac atcacggtcg acaacggctg ggtcatgagc cgctcgcaac cggcgaagcg
     4561 cgatccggcg gcggcgatca tcgcgcgctc aggcggcaag ccgatcgaag ccacctcgac
     4621 ggtcaaaccc ggcgacgaga agcagcagta ctcgtttcac tcgtttggcg cggtgttcgt
     4681 cgaagtgcat gtcgatgccg acctcggcac gattcgcgtg gcgcgcatcg tcggcgtgta
     4741 cgacatcggg cgcgtactga acgagaagac cgcgcacagc cagttgatgg gcggcatggt
     4801 atggggcgtg ggggcggcac tgcaggaaga gaccttgctc gatacgcgct acgggcgttt
     4861 caccaacgca aatctcgccg agtaccacgt accggtgaat gccgatatcc gttcgctcga
     4921 catcacgttt atcgatgtgc ctgatccgca catcaactcg ctcggcgtgc gcggcatcgg
     4981 cgagatcggc atcaccggcg tgccggcggc gatcgccaat gccgtgtatc acgcgaccgg
     5041 cgtgcgcgta cgcgatctgc cggtgacgct cgataaggtg atgggggtga tgcaggtgtg
     5101 aatattggcg cttgcctgtc gatgcgttgc accgcacaat cggccttatt ctttctaccg
     5161 atgcccgttc atggcttacg cttcgctcgc cactggcata ttgctcgccg ctggtctcgg
     5221 ttcacgcttc gacccggacg gcctgcagaa caaactgctc gcccgcatgc ccgacggtac
     5281 gccggtcgcg caggaggccg cccaccggct gctgaaggtc gtcacgcgcg tgctggccgt
     5341 ggtgcggccg ggcgcggaaa cgctcgcacg cctcctgaac gacgccggct gcgacgtggt
     5401 attcgccgcc actgctgagc gtggcatggg cgcgagcctc gccgtcggca tcgaagccag
     5461 cgacgacgcc gagggctgga tcgtcgcgct tgccgacatg ccgcgcatcg ccacctcgac
     5521 gatcgaagca gtggcgcaag cgctcaacgg cggcgcttca atcgtcgcgc cgttttatca
     5581 agggcagcgc ggccatccgg tcggcttcgg catcgaacat cgggacgccc tcatggcgct
     5641 cgacggcgac accggcgcgc gcgcgctgct gatgtcgcag cgtgtggcgc ggctcgacgt
     5701 cgacgacccc gggattctgc gcgatgtgga tacgccggaa gatctgcgca acatttagct
     5761 ttagcgcatc tgtcaggtgc aaccttgctg aaccatccag tgcacccaag ggcacgtcac
     5821 gctgcggcat cggcatgacc gccaggcttg atccaggcct gcatttgggc gcatactgta
     5881 tatccataca gtattttagc cgcatgggtt ggcgcatcac gctgcgtgca tccacggcgc
     5941 gtcagcatga aacgcatttc gatcgaaccg tctttcgccg tatggcggcg cgccgcgcgg
     6001 gtgctgctgc ggcaaggtgt cgagccgtca cagatcgaat gggttgaatc cggaagcgag
     6061 gcagcggttg caagcccggg ggctggccat gatgcgggtg ggggccacgc tgtgcaatct
     6121 tcggcaacga ctgtggttga gcgtctcgcc agcaatctct ccagctccag cgcgagcgcc
     6181 gtcggcgagc cagacgcaga gtccacccgc accgccgcgg acagcctcgc cacaagcact
     6241 gccggcgtac tcgccattcc gcgcgaactg ctctcgtggc tcagaacggc tgcgtgtttt
     6301 cgcgcaccgg accgctggtc gctggtgtac cgcgtcttat ggcgctggac gcacggcgag
     6361 cgcaaggtcc tcgatctcga cgaccccgac ggcgcgctgc tcgatcaacg catccacacg
     6421 gtcgaacacg aaaccgacga tttgctgaca ctcacgttgt tcagacgtcg cgatccgtcg
     6481 atggggcctc ccgaattcgt cggctggtac gagccgcatc atgatctgct ggagcgcgcc
     6541 gccgcgcggt tcgccgcccg catgggcgaa tccacgtgga tgctggccac gccgcacggc
     6601 gcggtgttct ggaacggcat gctgctgcgc atcggccggc cgttagtgga ggaacgcgag
     6661 caaacctcgc aggcgttaac ggccagcgcc atgaccggcg aagccgtcac cagcactccc
     6721 acggaggcgc tctggctcgc gtattacgcc agcgccttca atgcccagcc ttcgcccgtg
     6781 ccgttgcgct attggagaat gccgcccgcg ggtccaccgc tgcccgcaca cctggcgcgc
     6841 aagcgcagcc gtctgggcgc gcagagcacg gcgagcaccg tcccgcccac gccgccggtc
     6901 gagtattcgg cgatgacgcc gcccttgcgg gaacccactg gcccgctcgc cacttgcagg
     6961 cgctgcgcgt tatggcgcaa tgccaaacag gcggttgcgg gcgccgggcc cgcccatgcg
     7021 gcgatcatgg tgctcggtga acagccgggc gagcacgaga accagcacgg cgaacccttc
     7081 acggggccgg ccggccaatt gctcgacgcc gtgcttgcgc gcgccggtct ggagcggcag
     7141 gcgctgtatc tgacctatgc cgtcaagcat tacaaatggg aaacgctcga cggagcgcgc
     7201 gtccaccgca cacccgtgcg gcgcgaagtg gaggcttgcc agtactggct ggaaaaagaa
     7261 ctgacgcgaa tcgcgccacg cgtggtcgtc acactcggcg caactgcgct gagagcgctg
     7321 accggcgcgc acgtcaatct gtccgagtac ctcggtcaaa ccattgccca cgaggggcgc
     7381 ctgatcgtgc cggcctggca tccctcgtat gcgctgggag tggtggacgc cacgttgcgc
     7441 gaagatgtcg tggctgccat tgtgacggcg ttcagccgtg cggcggcgct ggcggctgag
     7501 ggggcgtagc cggcaggata aaaaacctaa ccgctcatat acgagcggtt acgcgtggcc
     7561 gacagcgcgc ttgctgcctg ctgcctgctg cctgccaaag gtgagcatgt tcgggacata
     7621 ccgaatacgc aacggtgagc ctctacggga agccaaagcg aaacccatcc attcaggcat
     7681 accgcttgcg cgcctcatat cggccgcgca agctgcgcca attccgccta agctgatgtg
     7741 acgccgcgac tccagcaagc ctgtcgcgcg ccacattgaa gcgttgaatg cggtccgcgc
     7801 gtttcgaaac catccccgtc acatgagcga aaccactcca cgccttttca tcgtgtcgcc
     7861 ccatttcgac gacgccgtgt tcagttgcgg cgcgttactc gccgcgcatc ctgacgtcgc
     7921 ggtttgcacg gtgttcgccg cgccgcccga gcaggagatg tataccgaat gggatgaaaa
     7981 gtcgggtttc acgagcgccc atcaggccat ccgggcccgc acgctcgaag acaacctcgc
     8041 gcttgaggtg ctcgacgcga ttccgctgcg tatgccgttt cgcgacggcc agtacttcga
     8101 ctcgccgtcg atcagcaagc tggcggcggc gctcgaagag acgatctacc gcaccacggc
     8161 caacacgctg ctgatgccgc tcggactgca tcacgacgac cacgcgctgg tgttcgaagc
     8221 ctgctgcgaa atcctgccgc gcctgtcgca tctcacgttg ttcgcctacg aggacgcgat
     8281 tcaccgccgc acgcccggtg tcgtgcaggc gcggcttgcc gacctggcgc aacgcggcgt
     8341 cgtggcgaca ccggcgtatc cgagcgcggg ctacacgatc gacccgccac gccgcgcgca
     8401 gctcaaatgg gaggcggtca acgcctacgc aagccagctg cgcgctttcg gcgcgggcaa
     8461 ttacgatgac attttcgccg ccgaacgtta ctggcaactg agcgtaggcc gccgcgcgaa
     8521 aaagtaaggg ccgcgtaatg tgctggatcg actacgcttg attcagtcgc ttctcaacca
     8581 gaaggtgatg caatgagcta cgacatgcat accagtccga ttcccgatcc gaacgccgac
     8641 ccgaaccgcg acccggaagc cgatccattg gtgccgccat cgccgggtca tcacaccgag
     8701 gagccgcagc ggcccgatgg accgccggac aaagacccgg tgtaaacgcg ggcggcgtgg
     8761 caattggaaa gcatgtgtcc gaaacgccgg ccgcccgtga gttaaggcgg cggcgtgcgt
     8821 ggacaagcgc atcggctcgc taaaaaaccc cgcagcaaac cggggcggcg cgcgttagcg
     8881 cgtcagctcg gtaaagcctt gcaagagacg ctcgatgtcg gtggcgtgga tgttgcccat
     8941 cgtcgagatg cggaacagtt cggccgacaa gccgccttga ccggcataga tgacgaaacc
     9001 gcgcgccttc agcgcgtcgt gcagccgcgg atacgccacg ccctccggca agcgatacgc
     9061 gcgcagcacc accgatgact gctccggcgc caacacactg ccgattcccc gctccgccag
     9121 gcccgcgcga gcctgctc
//
LOCUS       DATASH010000226         2883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1809377,
            whole genome shotgun sequence.
ACCESSION   DATASH010000226 DATASH010000000
VERSION     DATASH010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1809377"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="VHV99_06335"
     CDS             <1..878
                     /locus_tag="VHV99_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589597.1"
                     /note="member of the Gnt family of gluconate transporters;
                     not involved in gluconate transport; unknown function;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="permease DsdX"
                     /protein_id="HEX3635095.1"
                     /translation="GRTIAYGLIVCVPTAIVAGPLFALLISRYITLPKENALAAQFLG
                     GADGNGGDAKNGAKANAANAAPKRELPSFGITLFTILLPVILLLIGSWADLVFTPKTL
                     ANDLLRFVGNSDVALLIAVLVSFWTFGASRGFNREQIQKFCGECLAPIAGITLIVGAG
                     GGFGRVLMDSGISKEIVNVATAMHLSPLLFGWLVAALIRLATGSATVAMTTACGIVAP
                     IASASGVQVTPELLVLATGSGSLIFSHVNDGGFWLIKEYFGMTVGQTFKTWSLLETII
                     SLMGLGLTFALATVV"
     gene            889..1392
                     /locus_tag="VHV99_06340"
     CDS             889..1392
                     /locus_tag="VHV99_06340"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEX3635096.1"
                     /translation="MILIAMGVSGAGKTRIGEMLAERLHCAFTDGDAFHSTANKEKMH
                     KGIPLTDDDRWPWLQTIRAAIEEKQNAAEEAVFTCSSLKRSYRDVLRGGGNGDKDVCF
                     VYLKGSFEVLRERLTTRTGHFFDPSLLQSQLDTLEEPGPDEAITVSIELSPEQIVDEV
                     LIQMKAR"
     gene            complement(1490..2878)
                     /gene="purB"
                     /locus_tag="VHV99_06345"
     CDS             complement(1490..2878)
                     /gene="purB"
                     /locus_tag="VHV99_06345"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009908754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEX3635097.1"
                     /translation="MSDTRPDTLFALNALSPLDGRYASKTEALRDWLSEAAFMRNRVT
                     VEIHWLIALSHAGFAEVPRFSEASEQFLLQLAERFTAHDAARIKDIERVTNHDVKAVE
                     YWLKESVKGQEELERASEFIHFACTSEDINNTSHGLMLAGAREHVILPALRSVHQRLV
                     ALAHAQAEQPMLSRTHGQPASPTTLGKEIANVAARLERAIDRIAKVELLGKMNGAVGN
                     FNAHLSAYPEFDWEAFSREVVEQRLKLAFNPYTIQIEPHDYMAELFDAVSRANTILLD
                     LDRDVWGYISVGYFKQRTKAGEIGSSTMPHKVNPIDFENSEGNLGLANATLRHLADKL
                     PISRWQRDLTDSTVLRNIGVAFGYSLLAYDSLIRGLDKLEVNAQRLNEDLDNCWEVLA
                     EPVQTVMRRYGIENPYEQLKELTRGKGITREALQTFVSGLAIPQDAKDRLLAMTPHSY
                     IGKAVELAKRIA"
BASE COUNT          528 a          826 c          927 g          602 t
ORIGIN      
        1 tcggcaggac catcgcctat gggctgatcg tctgcgtgcc gaccgcaatc gtcgccggtc
       61 cgctgtttgc gctgctcatc agccgctata tcaccttgcc gaaggaaaac gcgctcgccg
      121 cgcaattcct gggcggcgct gacggcaacg gcggtgacgc gaaaaacggc gcgaaagcca
      181 acgcggcaaa cgcagcgccg aaacgtgaat tgccaagctt cggcatcacg ctgttcacga
      241 ttctgctgcc ggtgattttg ttgctgatcg gcagttgggc cgatctcgtg tttacgccga
      301 agaccctggc gaacgacctg ctgcgtttcg tcggcaattc ggacgtcgcg ctgttgatcg
      361 ccgtgctggt cagtttctgg accttcggcg cgagccgcgg cttcaatcgt gagcagatcc
      421 agaagttctg cggcgaatgt ctggcgccga tcgcgggcat cacgctgatc gtgggcgcgg
      481 gcggcggctt cggacgcgtg ctgatggata gcggcatctc gaaggaaatc gtcaacgtgg
      541 caacggccat gcatctgtcg ccgctgctgt tcggctggct ggtggccgcg ctaatccgtc
      601 tggccacggg ttcggcgaca gtggcgatga cgaccgcctg cggtatcgtc gcgccgatcg
      661 cctcggctag cggcgtgcag gtcacgccgg aattgctggt gctcgccacc ggctcgggct
      721 cgctgatctt ttcgcacgtg aacgacggcg gtttctggct gatcaaggaa tacttcggta
      781 tgacagtggg gcagaccttc aagacatggt cgctcctcga aaccatcatt tcactgatgg
      841 gcttgggttt gactttcgcg ctcgcgacgg tcgtgtaagg agtttttgat gattttgatc
      901 gcaatgggcg tgtcgggcgc cggcaaaacg aggattggcg aaatgctggc ggaacgcctg
      961 cactgcgcat ttaccgacgg agacgcattt cacagcaccg ccaacaagga gaaaatgcac
     1021 aaaggcattc cgctcactga cgacgatcgt tggccgtggc tgcaaaccat ccgtgcggcg
     1081 atcgaagaaa agcagaacgc ggccgaggag gcagtgttca cgtgttcgtc gctgaagcgc
     1141 tcgtaccgcg atgttctgcg cggcggcggc aacggtgata aggacgtgtg cttcgtttat
     1201 ctgaagggtt cgtttgaggt gctacgcgag cggctgacca cgcgcacggg tcacttcttc
     1261 gatccttcgc tgctgcaaag ccagctcgat acactcgaag agccggggcc ggacgaggcg
     1321 atcacggtga gcatcgaatt gtcgccggaa caaatcgtgg atgaggtgtt gattcagatg
     1381 aaggcgcgat gagtttctgg ctcgcgtgtg cgggatgaaa aataggcgct ccgaggagcg
     1441 cctattttta tggctgaagc cagcaagaca agcagcgagc tttgagcgat caagcgatcc
     1501 gcttggccaa ttccaccgcc ttgccgatat acgaatgcgg cgtcatggca agcaaacgat
     1561 ccttcgcatc ctgcggaatc gcgaggccgc tcacgaacgt ttgcagcgct tcgcgcgtga
     1621 tgcccttgcc gcgcgtcagt tccttcagtt gctcgtacgg attctcgatg ccgtaacggc
     1681 gcatcaccgt ttgcaccggc tcggccagca cttcccagca gttatccaga tcttcgttca
     1741 gacgctgcgc attcacttcg agcttgtcca gaccgcggat cagcgagtcg tacgcgagca
     1801 gcgagtagcc gaacgcgacg ccaatattgc gcagcaccgt ggagtcggtg aggtcgcgct
     1861 gccagcgcga aatcggcagc ttgtcggcga ggtgccgcag cgtggcgttg gccagaccga
     1921 gattgccttc cgagttttcg aagtcgatcg ggttgacctt gtgcggcatc gtcgacgaac
     1981 cgatttcgcc ggccttcgtg cgttgcttga agtaaccgac cgagatgtag ccccatacgt
     2041 cgcggtccag atccagcagg atcgtgttgg cgcgtgacac ggcgtcgaac agttccgcca
     2101 tgtaatcgtg cggttcgatc tggatcgtgt acggattgaa cgcgagcttc aggcgttgtt
     2161 cgaccacttc gcgcgagaac gcttcccagt cgaattccgg atacgcggac agatgcgcat
     2221 tgaagttacc gaccgcgccg ttcatcttgc ccagcagttc gactttcgcg atgcggtcaa
     2281 tcgcgcgttc cagacgcgcg gcgacgttgg cgatttcctt gccgagcgtg gtcgggctgg
     2341 ccggctggcc gtgcgtgcgc gacagcatcg gctgttcggc ttgcgcgtga gccagcgcca
     2401 cgaggcgttg atgcaccgaa cgcagcgccg gcagaatcac gtgttcacgc gcgccggcca
     2461 gcatcaggcc gtgcgaggta ttgttgatat cttccgacgt gcaggcgaaa tggatgaatt
     2521 cgctcgcgcg ttccagttct tcctgaccct tcaccgattc cttcagccag tactcgaccg
     2581 ctttcacgtc atgattcgtg acgcgttcga tgtccttgat gcgcgcggcg tcgtgcgcgg
     2641 tgaagcgctc ggccagttgc agcaggaatt gttcggacgc ttcggaaaag cgcggcactt
     2701 cggcaaaacc ggcgtgcgag agcgcgatca gccagtggat ttcaaccgtc acgcgattgc
     2761 gcatgaaagc ggcttccgag agccagtcgc gcagggcttc ggttttcgag gcgtagcggc
     2821 cgtcgagcgg ggagagcgcg ttcagcgcga acagggtgtc ggggcgggtg tcggacatga
     2881 tgg
//
LOCUS       DATASH010000227         2775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2007544,
            whole genome shotgun sequence.
ACCESSION   DATASH010000227 DATASH010000000
VERSION     DATASH010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2007544"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /gene="carB"
                     /locus_tag="VHV99_06350"
     CDS             <1..924
                     /gene="carB"
                     /locus_tag="VHV99_06350"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004193866.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HEX3635098.1"
                     /translation="GVMEHIEQAGVHSGDSACSLPPYSLSKETIAELKRQTGAMAKAL
                     NVVGLMNVQFAIQQVPQADGSKEDIIYVLEVNPRASRTVPYVSKATSLPLAKIAARAM
                     VGQTLAQQGVMKEIDPPYFSVKEAVFPFVKFPAVDPVLGPEMRSTGEVMGVGQTFGEA
                     LFKSQLAAGSRLPESGTVLLTVMDADKPKAVVVARMLHELGYPIVATKGTAAAIEAAG
                     VPVKIVNKVKDGRPHIVDMIKNGEIALVFTTVDETRAAIADSRSIRMSAQANKVTYYT
                     TMSGARAAVEGLRYLKNLEVYDLQGLHARLN"
     gene            1076..1552
                     /gene="greA"
                     /locus_tag="VHV99_06355"
     CDS             1076..1552
                     /gene="greA"
                     /locus_tag="VHV99_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004534614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX3635099.1"
                     /translation="MSTVPLTKRGAEMLRDELQRLKSVERPSVINSIAEARAQGDLSE
                     NAEYDAAKEKQGFIEGRIAEIESKLAGAQVIDPSKVDADGRVVFGATLELEDLDSGAK
                     VKYQIVGDDEADIDHGLISISSPIARALIGKSEGDVASVQAPGGVREYEIIAVSYV"
     gene            1573..2082
                     /locus_tag="VHV99_06360"
     CDS             1573..2082
                     /locus_tag="VHV99_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HEX3635100.1"
                     /translation="MPHRLFRLLTVVWVGSLLTIGYAVAPVLFTSLDRMTAGAVAAQL
                     FRIEGVLGAVCGILLLVLANVLVRRGSDAYRRLRWLIAGMLVCVLVGYFALQPFMNAM
                     RVAALEAGSDVGHSAYATRFGILHGVSSLFYLIESLLGVALVWKLPASVGSLTAEQGA
                     RSAAGKVTG"
     gene            complement(2245..2775)
                     /locus_tag="VHV99_06365"
     CDS             complement(2245..2775)
                     /locus_tag="VHV99_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HEX3635101.1"
                     /translation="MPALKVSSDQRAELRSQAHALKPVVLVGAEGLTDAVLSEIKVHL
                     GAHQLIKIRVFGDEREERIAIYEQICDKLNAAPIQHIGKLLVIWKPEAAAQPAVKAKR
                     GALPSAREAAVEAKPGKGRAPRVVTVVKPSEIPMRKPKAKAVVVRGNERVTQGGNIKR
                     AKKRQTSAKRAHQSSK"
BASE COUNT          496 a          773 c          920 g          586 t
ORIGIN      
        1 ggcgtgatgg agcacatcga acaggcgggc gtccattcgg gcgactcggc ttgctcgctg
       61 ccgccgtact cgttgtcgaa ggaaaccatt gccgagctga agcgccagac cggcgccatg
      121 gcgaaggcgc tgaacgtggt cggtctgatg aacgtgcagt tcgcaattca gcaggtgccg
      181 caggcggatg gctcgaaaga agacatcatc tacgtgctgg aagtaaaccc gcgtgcgtcg
      241 cgcacggtgc cgtacgtgtc gaaggcgacc agcctgccgc tcgcgaagat cgcggcgcgc
      301 gcaatggtcg gccagacgct ggcacaacag ggcgtgatga aggaaatcga tccgccgtac
      361 ttcagcgtga aggaagcggt gttcccgttc gtcaagttcc cggcagtcga tccggtgctc
      421 ggaccggaaa tgcgctcgac cggtgaagtg atgggcgtgg gtcagacatt cggtgaagcg
      481 ctcttcaagt cgcagctcgc ggcaggttcg cgtctgccgg agtcgggcac ggtgctgctg
      541 accgtgatgg acgccgacaa gccgaaggcg gtcgttgtcg ctcgcatgct gcacgaactc
      601 ggctatccga ttgtcgcgac caaggggacg gctgccgcga tcgaagcggc cggcgtgccg
      661 gtcaagatcg tcaacaaggt gaaggacggc cgtccgcaca tcgtcgatat gatcaagaac
      721 ggcgaaatcg cgctggtttt cacgaccgtc gacgaaaccc gcgccgcgat cgccgactcg
      781 cgctcgatcc gtatgagcgc acaggcgaac aaggtcacgt actacaccac gatgtccggc
      841 gcacgggcag cagttgaagg tttgcgctat ttgaaaaacc tggaagtcta tgatttacaa
      901 ggcctccacg ctcgcctaaa ctaaggcttc gaattcctgt ccaagatgta agtgccgcgg
      961 ttaagcggcg ttccgcaggt gcttggcgcc gagctttgct cagcgctctg gccccggcgg
     1021 aatgcactta accgcggtga ttttttttac ggctgttttt tgcatagagt tgtttatgag
     1081 cactgttcca ttgacgaagc gcggcgcgga aatgttgcgc gatgaattgc agcgcctgaa
     1141 atcggttgag cgtccttcag tgatcaattc gatcgctgaa gcgcgtgccc agggcgatct
     1201 gtcggaaaac gcggaatacg acgcggcgaa ggaaaagcag ggcttcatcg aaggccgtat
     1261 cgccgaaatc gaatcgaagc tggccggcgc gcaggtgatc gatccgtcca aggtggacgc
     1321 ggacggccgc gtcgtgttcg gcgcgacgct cgaactcgaa gacctggatt cgggcgcaaa
     1381 ggtcaaatac cagatcgtcg gtgacgacga agcggacatc gaccacggtc tgatctcgat
     1441 cagctcgccg atcgcgcgcg cgttgatcgg caagtcggaa ggcgacgttg catcggtgca
     1501 agcgccgggc ggcgtgcgcg agtacgaaat catcgcggtt agctacgttt gaaggtggcc
     1561 acggtgtcct tgatgccaca tcgactgttc cgcctgctga cggtagtgtg ggtcggtagt
     1621 ctgttgacta ttggctatgc cgtcgcgccg gtgctgttca catcgctcga ccggatgact
     1681 gcgggcgcgg tggcggcgca actatttcgc attgaaggcg tgctgggcgc ggtgtgcgga
     1741 attttgctgc tggttctggc gaacgtactg gttcgccgcg gaagcgacgc ttatcgccgt
     1801 ttgcgctggt tgatcgccgg tatgcttgtt tgcgtgctgg tgggttactt tgcgttgcag
     1861 ccgtttatga atgcaatgcg cgttgccgcg ctggaagcgg gcagtgacgt cgggcattca
     1921 gcttatgcga cacgcttcgg catcctgcac ggcgtatcga gtctgtttta tctgatcgaa
     1981 agcctgctgg gcgtggcgct ggtgtggaag ctgccggcga gcgtcggcag tttgacagcg
     2041 gagcagggcg ctcgcagcgc tgcggggaaa gtaaccggtt gagtggcgcg gttacggctt
     2101 gcgtacgcgg gtttgcctgt agagttgctc aagcggaacg cccccggcgg ccttatctag
     2161 gtagagacaa ccaggtggaa gtggtcacgc agtctgagca gttcacgcca aggtgcggcc
     2221 accgcccgca ccctcatcat tcgcttactt cgacgactga tgcgcgcgct tcgcgctggt
     2281 ctggcgcttc ttggcgcgct tgatgttgcc gccttgcgtg acgcgttcat tgccgcgcac
     2341 cacgactgct tttgccttcg gcttgcgcat tgggatttcg ctgggtttca ccaccgtgac
     2401 cacgcgcggc gcgcggcctt tacccggttt ggcttcgaca gccgcttcgc gggcgctcgg
     2461 cagggcgccg cgcttggcct tcacagccgg ttgtgcggct gcttccggtt tccagatcac
     2521 cagcagctta ccgatatgct ggatcggtgc tgcattcagc ttgtcgcaga tctgttcgta
     2581 gatggcgatg cgttcttcgc gttcgtcgcc gaacacgcga attttgatca gttgatgcgc
     2641 gccaaggtgg accttgattt ccgacagcac agcgtcggtc aagccttcgg cgccgacgag
     2701 cacgaccggt ttgagcgcat gtgcctggga gcgcaactcg gcgcgttggt cggaagagac
     2761 tttaagggct ggcat
//
LOCUS       DATASH010000228         4810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1809497,
            whole genome shotgun sequence.
ACCESSION   DATASH010000228 DATASH010000000
VERSION     DATASH010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1809497"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="VHV99_06370"
     CDS             <1..737
                     /locus_tag="VHV99_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009932860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX3635102.1"
                     /translation="THGVNFDAMLSALNSYAAGTVVVLHACCHNPTGVDLNVDQWKQI
                     VEVVKARNLVPFLDIAYQGFGDDIDSDAAAVRLFAASDLNVFVTSSFSKSFSLYGERI
                     GALSIITASKEESARVLSQLKRVIRTNYSNPPTHGGSVVAAVLASAELRATWETELAE
                     MRDRIRAMRNGLVERLKASGVDRDFSFVNAQRGMFSYSGLTAPQVDRLRDEFSIYAVS
                     TGRICVAALNTRNLDLVAGAIAHVLK"
     gene            complement(964..2202)
                     /locus_tag="VHV99_06375"
     CDS             complement(964..2202)
                     /locus_tag="VHV99_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX3635103.1"
                     /translation="MAQRNLKQARVGAPGGGEGEGAGPAPAAHPGRHLHLPNYETVAL
                     MLQGGGALGAYQAGVFQGLYEAGIEPNWLAGISIGALNTAIIAGNPPEKRVERLLQFW
                     ETICQPAFGLPLPAFIEHALFNSSDAIRKAFTATQAMGAIVEGQKGFFVPRFPPPLPT
                     VSGPPQLASYYDTTPLKATLEALCDFDRINSGEMRVSVGAVNCGTGNFAYFDNTHTKL
                     KPEHFMASGALPPGFAAVEIDGQYYWDGGLMSNTPLYEVIQSTPRHDTLAFQVDLWSA
                     IGPVPDNITDVQGRMKDIQYSSRTRLVTDMLQRSQRFRHVLREVLDRVPSDQRDDPWC
                     KLADDLSCSKRYNVIHLIYRHKEYEGHYKDFQFGLSTMREHWQSGLEDIRHSLAQPDW
                     LAMPDNDSGFVTHDIHRDAR"
     gene            complement(2213..3010)
                     /locus_tag="VHV99_06380"
     CDS             complement(2213..3010)
                     /locus_tag="VHV99_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HEX3635104.1"
                     /translation="MSSLNTNLNGKVAVVTSAASGIGRQIALTLSAAGAAVAIADLNQ
                     DGANAVAEEIKKGGGKAIGVAMDVTNEDAVNQGIDKVAAEFGSIDILISNAGIQIVNP
                     IENYSFSDWKKMQAIHVDGAFLTTKAALQHMYKDDRGGIVIYMGSVHSHEASPLKSAY
                     VAAKHALLGLSRVLAKEGAKHNVRSHVVCPGFVRTPLVDKQIPEQAKELGISEEDVIK
                     RVMLGGTVDGIFTTVEDVAQTVLFLSNFPTAALTGQSFIVSHGWYMQ"
     gene            3234..4205
                     /locus_tag="VHV99_06385"
     CDS             3234..4205
                     /locus_tag="VHV99_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006407924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX3635105.1"
                     /translation="MNYRRLGRSGLQVSELSIGSWVTYGNQVDHRAARESLAAARDAG
                     VNFFDNAEVYAAGQSEEIMGQALKELAWPRLSYVVSTKFFWGLNEAPNQYHTLNRKYL
                     LNAIDNSLQRLQLDYVDLVFCHRPDPNTPIEETVWAMSDMITRGKALYWGTSEWSADE
                     IRAAYDIAERHHLHKPVMEQPQYNLFHRKRVEQEYKRLYEDIGLGLTTWSPLASGLLT
                     GKYRDGVPADSRAQLQGYDWLRKQVTDAGKNNVVGKLGDVADELGCTVGQLAIGWILK
                     NPHVSTVITGASRVEQIGENMKSAEVAEQITPEIKQQIEEIIGDAYD"
     gene            complement(4320..4454)
                     /locus_tag="VHV99_06390"
     CDS             complement(4320..4454)
                     /locus_tag="VHV99_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635106.1"
                     /translation="MFQNVGVIACMEGVAIAEHDEETLKEGVPVQGGFGDASAMRQHN
                     "
     gene            4398..>4810
                     /gene="rlmJ"
                     /locus_tag="VHV99_06395"
     CDS             4398..>4810
                     /gene="rlmJ"
                     /locus_tag="VHV99_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006053107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEX3635107.1"
                     /translation="MLSYRHAFHAGNHADVLKHAVVLQLLRYLGQKDKAYWYIDTHAG
                     AGVYSLKEGYATKTGEFQTGIAKLWERNDLPPIFADYVDEVSALNPDGQLRFYPGSPY
                     IAWRQMREQDRMRLFELHTTEIDVLRHNFRDAGRRA"
BASE COUNT          971 a         1495 c         1422 g          922 t
ORIGIN      
        1 acacccacgg cgtgaatttc gacgccatgt tgagcgcgct taacagttat gcagcgggca
       61 cggtggtcgt gctgcacgcg tgctgccaca acccgaccgg cgtggacctg aacgtcgacc
      121 aatggaagca gatcgtcgaa gtcgtcaagg cgcgcaatct ggtgccgttc ctcgacatcg
      181 cgtatcaagg tttcggcgac gacatcgatt ccgacgctgc agcagtccgt ctgttcgcgg
      241 cctctgacct gaacgtgttc gtgacgtcgt cgttctcgaa gtcgttctcg ctgtacggcg
      301 agcgtatcgg cgcactgtcg atcatcacgg caagcaagga agaatcggct cgcgtgctgt
      361 cgcaactgaa gcgcgtgatc cgcacgaact actcgaaccc gccgacgcac ggcggctcgg
      421 tggtcgcagc ggtgctcgca tcggctgaac tgcgcgccac gtgggaaacg gaactcgccg
      481 aaatgcgcga ccgcatccgc gccatgcgta acggtctggt cgaacgtctg aaggcaagcg
      541 gcgtggatcg cgatttcagc ttcgtgaacg cacagcgcgg catgttctcg tactcgggcc
      601 tgacggcgcc gcaagtggac cgtctgcgtg atgagttcag catctacgct gtgagcaccg
      661 gccgcatctg cgtggctgcg ctgaatacgc gcaacctcga cctggtggct ggcgcaatcg
      721 ctcacgtgtt gaagtaaggc attcggcgcg cgagccacta ttgcgcgcgc attgctttgc
      781 tgaggccgtt tgggctgctc acgttacttg agctgatcag gctgcttgaa ctgcttgtag
      841 aaacggcgcc catttcgggc gccgtttttt attggctgct gattgtctga cgcgcgtatc
      901 gtcggtatag cgtccacaca acgcagctgg ttggttcaac gcacggcgcg agctactacc
      961 gcttcagcgc gcatcccgat gaatatcatg cgtgacaaac cctgagtcgt tgtcaggcat
     1021 ggccagccaa tcgggttggg caagcgagtg acgaatatct tccaggccac tctgccagtg
     1081 ctcacgcatt gtcgacaaac cgaactggaa gtccttgtag tgcccttcgt actctttgtg
     1141 ccggtagatg agatggataa cgttgtagcg cttcgaacac gatagatcat ccgccagctt
     1201 gcaccacgga tcgtcgcgct ggtcagacgg cacgcgatcg agcacctcgc gcagcacatg
     1261 ccggaatcgc tgcgaacgtt gcagcatatc cgtcacgaga cgcgtgcggc tcgagtactg
     1321 gatatccttc atgcgccctt gaacatcagt gatattgtcc ggcaccggcc cgatcgcgct
     1381 ccatagatcg acctggaacg cgagcgtgtc gtgacgcggc gtggactgaa tcacctcgta
     1441 gagcggcgta ttcgacatca agccgccatc ccagtagtac tggccgtcga tttcgactgc
     1501 cgcgaaaccc ggcggcaacg cacctgacgc catgaaatgc tccggcttca gtttcgtatg
     1561 cgtgttatcg aagtacgcga aattacctgt accgcaattc accgcaccca ccgacacacg
     1621 catctcaccg gaattgatcc ggtcgaaatc gcataaagct tcgagcgtcg ctttcagcgg
     1681 cgtggtgtcg tagtagctcg ccaactgcgg cggcccggaa acggtgggca acggcggcgg
     1741 aaaacgcggc acgaaaaagc ccttttgccc ttccacgatc gcgcccattg cctgggtggc
     1801 cgtgaaggcc ttacgaatgg cgtcgctgga attgaacagc gcgtgctcga taaaagccgg
     1861 caacggcagc ccaaacgccg gttgacagat cgtctcccag aattgcagta gccgctcgac
     1921 gcgtttctcg ggcggattcc cggcaatgat ggccgtgttc aatgcgccaa tcgaaatgcc
     1981 ggcaagccag ttcggctcga tgccggcttc gtaaagtccc tgaaacacgc cggcctgata
     2041 ggcacccagc gcgccaccgc cttgcagcat caacgcgacg gtttcgtagt taggcaaatg
     2101 gagatgccgg ccgggatgcg cggcgggcgc tggaccggca ccctcgcctt ccccgccgcc
     2161 gggcgcgccg acgcgcgcct gcttgagatt gcgttgcgcc acgggcgcct ccttattgca
     2221 tataccagcc gtggctcaca ataaaggact ggccagtcag cgccgcagtc gggaaattgg
     2281 acaggaacag caccgtttgc gcgacgtctt ccaccgtggt gaaaataccg tcgaccgtgc
     2341 cgcccagcat cacgcgcttg atcacgtctt cttcgctgat gccgagctct ttggcttgct
     2401 cgggaatctg cttgtcgacg agcggcgtac gcacgaaacc cggacacacc acatgcgagc
     2461 gcacattgtg tttcgcgcct tccttggcca gtacacgcga aagccccaac agcgcatgct
     2521 tggccgccac ataggcggac ttcagcggcg acgcttcgtg cgaatggacc gagcccatgt
     2581 agatcacgat gccgccgcga tcgtccttgt acatgtgctg gagcgcggcc ttggtggtaa
     2641 gaaaggcgcc atccacgtgg atcgcctgca tcttcttcca gtcggaaaac gagtagttct
     2701 cgatcggatt gacgatctga atgccggcgt tggaaatcag aatgtcgatc gagccgaact
     2761 cggccgcgac tttgtcgatg ccctggttga cggcgtcttc gttcgtgacg tccattgcca
     2821 cgccaatggc ttttccgcca ccctttttga tttcttcagc gacggcgttc gcgccgtcct
     2881 ggttcaggtc ggcgatcgcg accgccgcac ccgcggcgga cagcgtgagc gcaatctgcc
     2941 tgccgatgcc gctcgcggcg ctggtaacca ccgcaacctt gccattcaga ttcgtgttca
     3001 gtgacgacat ccagaacctc catgcagttg atgagcaatg agaccacggg ccgcaggcgg
     3061 cgtgaagtcc gccaaacggc ataggccgtt tggccgaatg ctgcactgcg gccaacgctg
     3121 ctattgtgca tgaaccgtgt gaactgcatc cgtaaaccgc acatcgagcc ttatctcttt
     3181 aaactagggg ttttcagcgg gattccggcg gcatcactac caaggaggaa tgcatgaatt
     3241 atcgacgtct gggccgttcc ggcctgcaag tcagcgaact gtccatcggc tcatgggtca
     3301 cctacggcaa tcaggtagat caccgggcgg cgcgcgagtc ccttgcggcc gcgcgcgatg
     3361 cgggagtcaa cttcttcgac aacgcggagg tatacgccgc gggccaatcc gaagaaatca
     3421 tgggtcaggc gctcaaggaa ctggcgtggc cgcgcctgag ctatgtggtc tcgacgaaat
     3481 tcttctgggg gctcaacgaa gcgccgaacc agtaccacac gctgaaccgc aaatatttgc
     3541 tgaacgcgat cgacaactcg ctccaacgcc tgcagctcga ttatgtcgat ctggtgttct
     3601 gtcatcgtcc tgacccgaac acgccgatcg aagaaaccgt ctgggccatg agcgacatga
     3661 tcacgcgcgg caaggcactg tactggggga cttctgaatg gagcgccgat gaaatccgcg
     3721 cggcttacga tatcgcggag cggcatcacc tacacaagcc ggtcatggag cagccgcagt
     3781 acaacctgtt tcaccgcaag cgcgtcgagc aggaatacaa gcggctttac gaggatatcg
     3841 gccttgggct caccacatgg agtccgctcg cgtccggctt gctcacgggc aagtaccgcg
     3901 acggcgtacc tgccgatagc cgcgcgcaac tgcaaggcta cgactggctg cgcaaacagg
     3961 tcaccgatgc cggcaagaac aacgtggtcg gcaagctggg cgacgtggcc gacgagctgg
     4021 gctgtacggt cggccagttg gcgattgggt ggattctgaa gaatccgcac gtgagcacgg
     4081 tcatcaccgg cgcgtcgcgc gtcgaacaga tcggtgagaa catgaaatcc gccgaggtgg
     4141 ccgagcagat cacgccggag atcaagcaac agatcgagga aatcatcggc gacgcctacg
     4201 actgaccggc cgctcgccgg accgcggagc atggcccgcg caccatccgg gccgtgccac
     4261 gcgcaacgca ctactgaggg caacgcggcg tcgcgtacaa tacgcgaccg cgccgcactt
     4321 cagttgtgct gccgcattgc actcgcatcg ccgaatccgc cctggaccgg cacgccctct
     4381 ttcagcgtct cctcatcatg ctcagctatc gccacgcctt ccatgcaggc aatcacgccg
     4441 acgttctgaa acacgccgtc gtgttgcagc tactgcgcta cctcggccag aaggacaaag
     4501 cctactggta tatcgacacg cacgcgggcg ccggcgtcta ttcgctgaag gaaggctatg
     4561 cgaccaaaac cggcgagttc cagactggca tcgccaaact atgggagcgt aacgacctgc
     4621 cgccgatctt cgcggattac gtcgacgaag tgagcgcgct gaatccggac ggccagttgc
     4681 gcttctaccc gggttcgccg tatatcgcgt ggcggcagat gcgcgagcag gaccgcatgc
     4741 gcctgttcga actgcacacc actgaaatcg acgtgctacg ccataacttc cgcgacgcag
     4801 gccgccgcgc
//
LOCUS       DATASH010000229         6075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_28848,
            whole genome shotgun sequence.
ACCESSION   DATASH010000229 DATASH010000000
VERSION     DATASH010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6075
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28848"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..202)
                     /gene="thiS"
                     /locus_tag="VHV99_06400"
     CDS             complement(5..202)
                     /gene="thiS"
                     /locus_tag="VHV99_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEX3635108.1"
                     /translation="MDIHINQKPLSLPEGATVADALAAYGARPPFAVALNGDFVARSQ
                     HAARALEPGDRLDVVQPVAGG"
     gene            complement(289..1419)
                     /locus_tag="VHV99_06405"
                     /pseudo
     CDS             complement(289..1419)
                     /locus_tag="VHV99_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006052268.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     regulatory      complement(1657..1780)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2158..3909
                     /locus_tag="VHV99_06410"
     CDS             2158..3909
                     /locus_tag="VHV99_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEX3635109.1"
                     /translation="MTSNTASSPALPPDEKVSAWSLIKPYWVSEERKTAWGLLIAIIV
                     MNLLVVWINVRLNRWSADFYNALQTKNVHDFPHLLMVFSGLAFGFIILAVYGRYLRQM
                     LGFRWRQWLTTRYLNEWLHDSAFYRIERDRLTDNPDQRISDDLQSFATTTLSLTLDLL
                     STVVTLVSFITILWSLAGALTISLGGMPLQIPGYMVWAAALYAVVGSLIIQKVGHPLV
                     SINYQAQKVEADFRFGLIRLRENAEQIAFYNGMETEKTNTRTLFGRIRDNWWQVMKYT
                     KRLTFVLSFYGQIAIIFPLVVAAPRYFAGAFTFGVLMQISSAFGTVSDSFSWFINSYG
                     TLVEWRATVNRLREFRRIVHAPRLKESVSPATAHGGINLHFVDEDKLSTEGLQLALPN
                     GNPLSRIRDIAIKPGSRWLVRGPSGSGKSTLMRALAGLWPFGDGSIDAPVNARMMFIP
                     QVSYMPIGTLKAALTYPSSADTYTDEECREALVTCHLSEYADRLQESGHWTRVLSPGE
                     QQRLAGARVLLHKPDYLFLDEATSALDAENEARLYHLFTERLPKAAIVSVAHRESLAA
                     FHGDTLDVERSGEPIAA"
     gene            3933..4724
                     /locus_tag="VHV99_06415"
     CDS             3933..4724
                     /locus_tag="VHV99_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3635110.1"
                     /translation="MSSTTGTSGAELDRVVLITGAGSGIGAALARRIAAPRTALMLHA
                     RGADDEARQRLALVAADCSANGAHCATVFGDLAERGAAEHVIHQTLASFGALDQLVAN
                     AGHAQRQTLNALDADAFSAAFAAMPAAFAALVKRATPALETSKRGRVVALSSFVAHRY
                     RADAPFAATAAAKAALESLAKTAAAELAPHGVTVNCVAPGYTRKDRGPSADNASVWTR
                     AAEATPLGHVAEPADIAALIAFLLSDEARHITGQVIHIDGGLTLG"
     gene            4833..5573
                     /locus_tag="VHV99_06420"
     CDS             4833..5573
                     /locus_tag="VHV99_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3635111.1"
                     /translation="MNPSILVVDDDPVVRELVSGYLLGRGFKVSTLEHGMALQRTLQD
                     QRPALIVLDIMMPELDGISALRALRVAGDDIPVILLTARADPIDRVIGLELGADDYLG
                     KPFEPSELVARIRTVLRRRGTLAPSAPEQRAPYRFGRFEVNFPARELHHDGARIALRS
                     SEFAILKVFVTHEMTVLTRAQLLEKLHGNSELYRNRSLDVSIWRLRRLIEIDPSQPRY
                     VQTVWGRGYVFVPDGEIGAAQLGAPLPG"
     gene            5975..>6075
                     /gene="ompR"
                     /locus_tag="VHV99_06425"
     CDS             5975..>6075
                     /gene="ompR"
                     /locus_tag="VHV99_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090722.1"
                     /note="part of two-component system EnvZ/OmpR; regulates
                     transcription of outer membrane porin genes ompC/F; under
                     high osmolarity EnvZ functions as
                     kinase/phosphotransferase and phosphorylates OmpR; the
                     result is increased expression of ompC and repression of
                     ompF; also functions in regulation of other genes; forms
                     dimers upon phosphorylation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HEX3635112.1"
                     /translation="MNPQVLIVDDDPVVRDLLCKFLQTNGFDASVLH"
BASE COUNT         1074 a         2039 c         1905 g         1057 t
ORIGIN      
        1 gcgctcagcc gcccgccacc ggttgcacga cgtcgagccg gtcgcccggc tcgagcgcgc
       61 gcgccgcatg ctgtgagcgc gcgacgaaat cgccgttcag cgcaaccgcg aacggtggcc
      121 gcgcgccgta agcggcgagc gcatcggcga cagtcgcgcc ttcgggcaac gacaacggct
      181 tctgattgat atgaatgtcc atggtgttcg aatacggttc aatcggtggc ggtagcagcg
      241 cggcgtgctt atttgcgtgc ggttagttca tcagcaggcg cgcaacgctc atgccggctc
      301 ccgcgtggaa tccaggtgaa acagctcgct ccagcgcgcg gcactttgcc agtcagcgaa
      361 agcatcggcg tcaccgatgc ggccgtcgag cagcgccgcg gcgaagcgca cggcttcgtc
      421 ggcgacttcg ggcacgatca tgtagccgtg ccggtacagg ccgttcacgc gcactgtctg
      481 cgcgccatcc cacagcaggg ccggacgatg gtccggcaaa gtcgggcggc actgctaatt
      541 cagttcgagg atgcgcgcct cgccgaagcc gggatgcacg gaaaacgccg cgctcagcag
      601 ttcgagcgcc gagcgcacgc tgacgggcga catgtcctcg ccttccacct cggttgcgcc
      661 gatcacgtag agatcgttgg gcttcggcgc gatatacagc ggatagcgcg gatgcagcag
      721 gcgcaccggc cgcgtcagct tgatccccgg cgcatgcacg cgggcgactt cgccgcgaat
      781 gccgcgcagc gtgggcaaca ccggcttcgc gcccaaaccg cggcagtcga tcgtgatgcg
      841 cgcgggcggc agcgcatggt cgtcgaccgc tgtgttccag tgcgtttcga caccgcgctg
      901 ggcgaggccc gcggcgagcg cggccagcac ctggcggtta tccagctggc cttcgcgcgg
      961 cagcagccag ccttggttga aacgacccgc caacgccggc tcagcggcgc cgacctgagc
     1021 accggtcagc gtgacgaagc cgccgtcgag caattcagca ggcgcgttcg agcgcacccg
     1081 gcgctcaaac aacggcgctt cggtgcggtc ggcgtgatgc catacgacca gcgtgccatt
     1141 gcgctggaag aacaccggtt caggcaattc ggccagcact cgcggccagg cgtcgagcga
     1201 actcgcgccc agacgggtga tcagcagttc cgcactggcg gcttcggcaa gcggcgccag
     1261 catggcggcg gcgacccagg ccgcggcttg cgtgcccgcg gcatcgccgc gctcatagag
     1321 cgcaacacgg tgcccctgcc cggcgagacg ccacgcgacg aggcgcccgc acagcccccc
     1381 gccgagtacc gcgaaatcag gttgaacgga acgattcatc gcgcccaccc cgagtcgcaa
     1441 tggcaagcag ggctaaagca gcacggcgcg ccggcacaac tgacgagcgc gcggccgaaa
     1501 gcaaaacacg caaaacacat atctgaagaa taggcggtca tcgagtcctt tccgtacggc
     1561 caaaaacgca cgtacccagg acgaaaccgg cgggcaacgc cggccgggca acacgcgcgt
     1621 ccaatgacgg acgccgcccg gcggggaatg gaagactggc tgcttccgga aacttcccgc
     1681 gccggtatta cccggatcgg gtgcaaaggg tctctctcag cctcgccgcc gctgcgtgag
     1741 aatcacgcag cctgtgggca aagcacccct gtttcgtcga aggtcattag accataaaag
     1801 gcgcggcgcc cgcaaaccga tgggctagct ctcgcacgcc gttgatccgg caccgtcttt
     1861 cgcctcatca atacggccga gggcggcgct ctggcacaat gccaacaccg gatgccgcag
     1921 gagccacgcg cgtgcgacat gcttggctgc atgcgcgagg gcggcatcga ggcgaggcct
     1981 tgatgcggaa attaattttc acttaagggc ttcgcgccag aatcggacgc tcacccggac
     2041 gctagtcgtc gagcacgacg cccagggcac gcaccgcaaa cccgacgtag gccggcctga
     2101 cagaggccag cctcgatggg ccggccccgg atgggccagc acttcatcag gatgctcatg
     2161 acatcgaata ccgcttccag ccccgcgctg ccgccggacg aaaaagtctc cgcctggagc
     2221 ctgatcaaac cttattgggt ttccgaagaa cgaaaaacgg cgtgggggct gctcatcgcg
     2281 atcatcgtga tgaatctgct cgtcgtgtgg atcaacgtgc gcctgaaccg ttggagcgcc
     2341 gacttctaca acgcgctgca aaccaagaac gtccacgact tcccgcacct gctgatggtg
     2401 ttctcggggc tcgcattcgg cttcatcatt ctcgcggtgt acggccgcta tctgcgccag
     2461 atgctcggct ttcgctggcg ccagtggctg accacgcgtt atctcaacga gtggctgcac
     2521 gacagcgcct tctatcggat cgaacgcgac cggctcaccg acaatcccga ccagcggatc
     2581 agcgacgacc tgcaatcgtt cgccaccacc acgctctcgc tgacgctcga tctgctgtcc
     2641 acggtcgtca cgctggtctc gttcatcacg atcctgtggt cgctggccgg cgcgctgacc
     2701 atttcgctcg gcggcatgcc gttgcagatt ccgggttaca tggtgtgggc cgcggcactg
     2761 tacgcggtgg tcggctcgct gatcatccag aaggtcggcc atccgctcgt gtcgatcaat
     2821 taccaggcac agaaagtcga ggcggatttc cgtttcggtc tgatccgtct gcgtgaaaac
     2881 gccgaacaga tcgccttcta taacggcatg gagaccgaaa agacgaacac gcgcacgctg
     2941 ttcggccgca tccgcgacaa ctggtggcag gtgatgaagt acaccaagcg gctcaccttc
     3001 gtgctgagct tctatggtca gattgcgatc atctttccgc tggtggtggc ggcgccccgc
     3061 tacttcgccg gcgccttcac gttcggcgtg ctgatgcaga tctcgagcgc attcggcacc
     3121 gtcagcgatt cgttctcgtg gttcatcaac agttacggca ccctggtcga gtggcgcgct
     3181 accgtgaacc ggttgcgtga attcaggcgc atcgtgcatg cgccgcgtct gaaggaatcg
     3241 gtgtcgccgg ctaccgcgca tggcggcatc aatctgcatt ttgtcgacga agacaagctt
     3301 tccaccgaag gcctgcagct tgcgctgccg aacggcaacc cgctgtcgcg cattcgcgat
     3361 atcgcgatca aaccgggctc gcgctggctc gtgcgcggtc cgtccggttc gggcaagagc
     3421 acgctgatgc gcgcgctcgc cggcttgtgg ccgttcggcg acggctcgat cgacgccccg
     3481 gtcaacgcgc gcatgatgtt catcccgcaa gtgagctaca tgccgatcgg cacgctgaaa
     3541 gcggcgctca cctatccgtc ctccgcggac acctacaccg acgaagaatg ccgcgaagcg
     3601 ctcgtcacct gccacctgtc ggaatacgcc gaccgcctgc aggaatcggg acactggacg
     3661 cgcgtcctct cgccgggcga acaacagcgc ctcgccggcg ctcgcgtctt gctgcacaag
     3721 ccggactact tgttcctcga cgaagcgacc agcgcgctcg atgcggaaaa cgaagcacgt
     3781 ctctatcacc tgttcaccga gcggctaccg aaggcggcga tcgtcagcgt cgcgcatcgc
     3841 gaatcgctgg cggcgttcca cggcgacacg ctcgacgtcg agcgctcggg cgaaccgatt
     3901 gccgcgtgaa cagcgtgagc cagggaaacg acatgagcag cacgactggc acgagcggcg
     3961 ccgaacttga tcgcgtcgtc ctgattaccg gcgcgggttc cggaatcggc gcggcactgg
     4021 cccggcggat cgccgcgccg cgcaccgcgt tgatgctgca cgcacgcggc gccgacgacg
     4081 aagcgcgtca gcgtcttgcc ctggtggccg ccgattgcag cgcgaacggc gcacactgcg
     4141 cgaccgtgtt cggcgatctg gccgaacgcg gcgccgccga acacgtgatt catcaaacac
     4201 tcgcgagctt cggcgcgctc gatcaactgg tggcgaacgc gggccacgcg caacggcaaa
     4261 cgctcaacgc actcgacgcg gatgcgttca gcgccgcctt cgccgccatg cccgccgcgt
     4321 tcgccgcgct cgtgaagcgc gcgacgcccg cattggaaac ctcgaagcgc ggtcgcgtgg
     4381 tcgcgctcag ttcgttcgtc gcgcatcgct atcgcgcgga tgcaccgttc gccgccacgg
     4441 cggcggcgaa ggcggcgctc gaatcgctgg ctaaaaccgc ggccgccgaa cttgcgccac
     4501 atggcgtgac ggtcaattgc gtggcgcccg gctacacccg taaagatcgc ggccccagtg
     4561 ccgacaatgc gtcggtgtgg acccgcgccg ccgaagcgac gccgctcggt cacgtcgccg
     4621 agcccgcgga tatcgcggcg ctgattgcgt tcctgctttc cgatgaagcg cgccatatca
     4681 ccggccaggt gatccatatc gacggcggcc tcacgttggg ctaggcgaca ggccaactag
     4741 ccgcaccgcc tgtttgcggt tccttacgcg agaaaggatt cgaaagcgtt acgcgctttg
     4801 cgccgctcgc gtggctcact aagatgacct ggatgaaccc atccattctg gtcgtcgacg
     4861 acgaccccgt cgtacgcgag ctcgtcagcg gttatctgct agggcgtggc ttcaaggtgt
     4921 ccacgctgga acacggtatg gcgttgcaac gcaccttgca ggaccagcgt cccgcgctga
     4981 tcgtgctcga catcatgatg ccggaactcg acggcatcag cgcgctacgc gcattgcgcg
     5041 tcgccggcga cgatattccc gtgatcctgc taacggcgcg tgccgatccg atcgaccgcg
     5101 tgatcggcct cgaactcggc gccgacgact acctcggcaa accgttcgaa cccagcgaac
     5161 tggtcgcgcg gattcgcacg gttctgcgcc gccgcggcac gctcgcgccg agtgcgccgg
     5221 agcaacgcgc gccctatcgc ttcggccgtt tcgaagtgaa tttcccggcg cgcgaattgc
     5281 accacgatgg cgcgcgcatt gcgctgcggt cgagcgaatt cgcgatactc aaggtgtttg
     5341 tcacacacga gatgaccgtc ctcacgcgcg cgcaattgct ggagaaactg catggcaata
     5401 gcgagttgta tcgcaatcgc agcctcgatg tgtcgatctg gcgtttgcgg cggctgatcg
     5461 aaatcgatcc gtcccagcct cgttatgtgc aaaccgtctg gggacgcggc tacgtgttcg
     5521 tgccggacgg tgaaatcggc gcggcgcaac ttggcgcgcc gctgccaggt tagggctcgg
     5581 ctagtgcttg cacggtgttt gccctgtgct tggcctgtgc ttggcctgtg cttgccctgt
     5641 gcttgccctg tgcttgccct gtgtttgccc tgtgtttgcc ctgtgcttgc cctctttctg
     5701 ccccgtgcct ccccgtgtcc gccctgtgat tttgtcgtgc ctgtccagca ctttagagaa
     5761 gtcgcgcctg atggtcagcg agcccgtcgc gcaagtcccc ggcggtgatc ccggcgacca
     5821 cgatcccgtt gccgaatacg gcagccgcga actatccggt gagcccttgc tggcggccct
     5881 cgcacccgaa ccacccaaga cggcgcggct ttgcgtaagc gttggtaaga aaacagtcgg
     5941 tcgcgcgtcg cgcacgccag tctagaattt cgccatgaat ccacaggtcc ttatcgtcga
     6001 cgacgacccg gtcgtacgcg atctgctttg caagtttctg cagacgaacg gcttcgacgc
     6061 gtcggtgctg cacga
//
LOCUS       DATASH010000230         4546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5172165,
            whole genome shotgun sequence.
ACCESSION   DATASH010000230 DATASH010000000
VERSION     DATASH010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4546
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5172165"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1698
                     /gene="glnE"
                     /locus_tag="VHV99_06430"
     CDS             <1..1698
                     /gene="glnE"
                     /locus_tag="VHV99_06430"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEX3635113.1"
                     /translation="FLRELEHRLQYRNDAQTHAMPVDPEDRVALAHAMGCDNYAELML
                     KLDTHREFVEQQFDQIFADKVSGRDGCGAPEDGAAAWVWSSALADDSADEALQARLIE
                     LGVPQPGDLLARLRAVWQSSRYAGLAERSRQRFDIVAQRALEAARTLEPPERRGDTVA
                     RLFDLLEAVGRRGAYLALLTEYPRALHRVLSVLGASRWAAGYLIRHPQLLDELLDDEA
                     MDSPFDWPEFKRTLRLRLAAADGVEQQMDLLRHAHHAEVFRILLIDLAGRLSVEHVSD
                     RLSELADAVLDVTLEAVWRQLAKRHREVPKFAVIAYGKLGGKELGYASDLDVIFLYDD
                     PDDAAADVYATYTRRLISWLTTATGAGTLFDVDLRLRPNGESGLLVTDLDAFRRYQLR
                     EGDAANTAWVWEHQALSRARYCAGDAEIGAKFEQIREQVLTTPREAAPLAREIVEMRA
                     RVEAGHPNHTALFDLKHDRGGMVDIEFTVQYWVLLHAASDPELIRNTGNIALLREVSR
                     FGLMSEAEAETVGAAYRTYRKLQHTLRLDGMEKARVDPALVVTEREAVLALWERVFG"
     gene            complement(1800..3470)
                     /gene="recN"
                     /locus_tag="VHV99_06435"
     CDS             complement(1800..3470)
                     /gene="recN"
                     /locus_tag="VHV99_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEX3635114.1"
                     /translation="MLRHLSIRDFVIVAALDLEFDSGFTVFSGETGAGKSILIDALAL
                     ALGARADASVVRTGESRADITAEFEAHAQVEQWLDEQALGTSGDDGHHGATVMLRRVV
                     DVNGRSRAFINGTAATLAQLREVGEMLVDIHGQHAHQLLMRPDAQRELFDTHAGLTET
                     AATVTRTWRAWREKVQAVEHAQTRDRELQLERERLAWQLTELDKLSPQPGEWEEVNAE
                     HRRLSHSANLIDGVQGALSALSESDEAMITHLASIVSKVRDLAEIDPALNDVLAALEP
                     AEIQLQEAAYSLSHYAQKLDLDPDRLAQVEKRLDALHSAARKFRLQPETLPEEHEARR
                     AQLAALDAAADLDSLHAAVAKAKEAFLTEAKKLSKARAKAGKALGSAVTTGMQELSMK
                     GGSFEVALVPLPEGGAHGLEQVEFRVAGHAGVPLRPLAKVASGGELARISLALAVIAS
                     AASPIPTLIFDEVDTGIGGGVAEVVGRLLHQLGQARQVLCVTHLPQVAARGDHHFQVA
                     KAGNGKGGTVSSVTSLDRANRVEEVARMLGGLEITATTRKHAKEMLAA"
     gene            complement(3590..4492)
                     /locus_tag="VHV99_06440"
     CDS             complement(3590..4492)
                     /locus_tag="VHV99_06440"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEX3635115.1"
                     /translation="MQVTSQFKTVALVGRNNTPGIGEPLTALASCIAKRGFDVVFEAE
                     TAAEIGVTDYPALRPAEIGARADVAVVLGGDGTMLGIGRQLAPYRTPLIGINHGRLGF
                     ITDIPISDMREIVPQMLSGNFEREERVLLEARIMRGGNPIYHALAFNDVVVNRSGFSG
                     MAELHVSVDGRFMYNQRSDGLIVATPTGSTAYALSSQGPILHPQLQGIVLVPIAPHAL
                     SNRPIVLPDDSKVSIQIVSGREVNVNFDMQSFTSLELGDTIEVRRSRHTVPMLHPVGY
                     SHYATLRKKLHWNEYPSHEEDSKP"
BASE COUNT          724 a         1455 c         1542 g          825 t
ORIGIN      
        1 ttcttgcgcg aactcgagca ccgtctgcaa taccgcaacg acgcgcagac ccacgcaatg
       61 ccggtcgatc ctgaggaccg tgtggcgctc gcgcacgcca tgggttgcga caactatgcc
      121 gagctgatgc tcaagctcga cacgcatcgc gagttcgtcg agcagcagtt cgaccagatt
      181 ttcgccgaca aggtgagcgg ccgcgacggc tgcggcgcac cggaagacgg cgcggcggcg
      241 tgggtgtgga gcagcgcgct cgccgacgac agcgccgacg aagcgcttca ggctcgcctc
      301 atcgaactcg gcgtgccgca gccgggcgac ctgctggcgc ggttgcgcgc cgtgtggcag
      361 tcgtcgcgtt atgcggggct cgccgagcgt agccggcaac gcttcgacat cgtcgcgcag
      421 cgtgcgctgg aggccgcgcg cacgctggag ccgcccgaac ggcgcggcga cacggtggcg
      481 cgtctgttcg acctgctcga agccgtgggc cggcgcggcg cgtacctcgc gctgctgacc
      541 gaatatccgc gggcgctgca tcgggtgctg tcggtgctgg gtgcgtcgcg ctgggccgcc
      601 ggctatctga ttcgccaccc gcaattgctc gacgaactcc tcgatgacga ggcgatggac
      661 agtccgttcg actggcccga attcaagcgc acgctgcgcc tgcgcctcgc tgccgccgac
      721 ggcgtcgaac agcagatgga tctgctgcgc cacgcgcacc acgccgaggt gttccgcatc
      781 ctgctgatcg atctggccgg caggctgagt gtcgagcatg tgagcgaccg tctgtccgag
      841 ctcgccgacg ccgtgctcga tgtcacgctc gaagcggtct ggaggcaact ggccaaacgc
      901 caccgcgagg tgccgaagtt cgcggtgatc gcatacggca agctgggcgg caaggaactg
      961 ggttacgcgt ccgacctcga cgtgattttt ctctacgacg atccggacga tgccgccgcc
     1021 gacgtctacg ccacctatac ccgccggctc atcagttggc tgacgacggc gaccggcgcc
     1081 ggcaccttgt tcgatgtcga cttgcggctg cggccgaacg gcgagtcggg cctgctcgtc
     1141 accgatctgg atgcgttccg ccgttatcaa ctgcgcgagg gcgatgccgc caatacggcg
     1201 tgggtgtggg agcatcaggc attgagccgg gcgcgctact gcgcgggcga cgccgaaatc
     1261 ggcgcgaaat tcgagcagat ccgcgaacag gtgctgacca cgccgcgcga agccgcgccg
     1321 ctcgccaggg aaatcgtcga gatgcgcgcg cgcgtcgagg cgggacatcc gaaccatacg
     1381 gcgctgttcg acctgaagca cgatcgcggc ggcatggtcg atatcgaatt caccgtgcag
     1441 tactgggtgc tgctgcatgc ggcgagcgat cccgaactga tccgcaatac cggcaacatc
     1501 gcgttgctgc gcgaagtgtc gcgcttcggc ttgatgagcg aggcggaagc ggagacggtc
     1561 ggggcggctt atcggaccta ccggaagttg cagcacacgc tgcggctgga cgggatggag
     1621 aaggcgcggg tcgatccggc gctggtggtg accgagcgtg aggcggtgtt ggcgctttgg
     1681 gagcgggtgt ttgggtgacg gccgatggcg cgaggtgtga gcggcgtcgc gcaagttgcg
     1741 cggcccgcag agcttgcctt gcgttccccg agggccgcga tgattcccgg cctgagtttc
     1801 tacgctgcca gcatttcctt cgcgtgcttg cgggtggtcg cggtgatctc cagtccgccg
     1861 agcatccgcg cgacttcttc gacgcggttc gcgcggtcca gcgaagtcac gctgctcacc
     1921 gtgccgccct tgccgttgcc cgccttcgcc acctggaaat gatggtcgcc gcgcgcggcg
     1981 acctgcggca agtgcgtgac gcacagcacc tggcgcgcct gccccagttg atgcagtagc
     2041 cgtccgacca cttcagcgac gccgccgccg attcccgtgt ccacttcgtc gaagatgagg
     2101 gttggtatgg gactcgcggc gctcgcaatg acagccaggg cgagactgat ccgcgccagt
     2161 tccccgcctg aggcgacctt cgcgagcggc cgcaacggca cgccggcatg accggcgacg
     2221 cggaattcga cctgttcgag cccgtgcgcg ccaccttccg gcaacggcac cagcgcgact
     2281 tcgaagctgc cgcctttcat cgacagttcc tgcatgccag tggtgacggc agagcccaga
     2341 gccttaccgg ccttggcgcg cgccttcgac agtttttttg cttcggtaag gaaggcttcc
     2401 ttggctttgg cgacggccgc gtgcaggctg tcgagatcgg ctgcggcgtc aagcgcggca
     2461 agttgtgcgc gccgtgcttc gtgttcttcc ggcagcgtct ccggctgcag gcggaatttg
     2521 cgtgcggccg aatgcaacgc gtcgagacgc ttttcgacct gggcaagcct gtccggatcg
     2581 aggtcgagct tctgcgcgta gtgactgagc gagtaggccg cttcctgcaa ctggatctca
     2641 gccggttcca gtgctgccag cacgtcgttc agcgctgggt cgatctcagc caggtcacgc
     2701 accttcgaca cgatcgaggc gagatgcgtg atcatcgctt cgtccgattc ggacaacgcg
     2761 ctgagtgccc cttgaacgcc gtcgatcaga ttcgccgaat gcgacaggcg ccggtgctcg
     2821 gcgttgacct cttcccattc gcccggttgc ggcgaaagtt tgtccagttc ggtgagctgc
     2881 caggcgagcc gctcgcgctc cagttgcaat tcccggtcgc gcgtttgcgc atgctcgacg
     2941 gcctgaacct tttcgcgcca cgcccgccag gtgcgcgtga cagttgcagc ggtttcggtc
     3001 agaccggcat gggtgtcgaa cagctcgcgt tgtgcgtccg gacgcatcag caactggtgt
     3061 gcatgctggc catgaatatc caccagcatc tcgccgactt cgcgcaactg cgcaagcgtc
     3121 gccgctgtgc cgttgatgaa cgcccgcgag cggccgttca cgtccaccac gcgccgcagc
     3181 atcaccgtgg cgccatgatg gccgtcgtcg ccggacgtgc cgagcgcctg ctcgtcgagc
     3241 cattgctcga cttgcgcatg cgcctcgaat tccgcggtga tgtcggcgcg gctctcgccg
     3301 gtgcgcacca cgctcgcatc ggcgcgggcg ccgagggcga gggccagcgc gtcgatcagg
     3361 atcgacttgc cggcgcctgt ttcgcccgag aaaacggtaa agccgctgtc gaattcgaga
     3421 tcgagcgcgg cgacgatgac gaagtcgcgt atcgagaggt ggcgaagcat ggaggtcgtc
     3481 tgatgagctt gaggttcagg gttcggcagc gatggtgttc gcgttccggt gttctggcgg
     3541 cccggcgatc cggcgatcca gcggtccagc gctccggtgt tcgggccgct cagggcttgg
     3601 aatcttcttc gtgcgacgga tattcgttcc agtgcagctt cttgcgcagc gtggcgtagt
     3661 ggctataacc gaccgggtgc agcatcggta ccgtatgacg cgagcggcgc acttcgatcg
     3721 tgtcgcccaa ttccagcgac gtgaacgact gcatgtcgaa attgacgttc acttcgcgcc
     3781 ccgagacgat ctggatgctc accttcgagt cgtccggcag cacgattggc cggttcgaca
     3841 gcgcgtgcgg cgcgatcggc accagcacga tgccttgcaa ttgcggatgc aggatcggcc
     3901 cctgcgagga cagcgcgtag gcggtcgaac ccgtaggcgt ggcgacgatc aggccgtccg
     3961 agcgctggtt atacatgaag cggccgtcca ccgagacatg cagttccgcc atccccgaga
     4021 aaccgctgcg gttgaccacc acgtcattga atgccagcgc gtgatagatc gggttgccgc
     4081 cgcgcatgat gcgcgcttcg agcagcacac gctcttcgcg ttcgaagttg ccggagagca
     4141 tttgcggcac gatctcgcgc atgtcggaga tcggaatgtc ggtaataaag ccgagccgcc
     4201 cgtggttgat gccgatgagt ggcgtgcggt agggcgctag ctggcggcca atgcctagca
     4261 tcgtgccgtc gccgcctagc accacggcca cgtcggcgcg cgcgccgatc tcggcgggac
     4321 gtagcgccgg gtagtcggtc acgccgattt cggcggcggt ctctgcttcg aacacgacgt
     4381 cgaagccacg ctttgcgatg cacgaggcga gcgcggtcag cggctcgccg atgcccggcg
     4441 tattgttgcg cccgacgagc gcgacggtct tgaactggct ggtcacttgc atgccggcat
     4501 tacaccatag gtagggcctg aaaagaaccg cagacagacc cggtag
//
LOCUS       DATASH010000231        14212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1613156,
            whole genome shotgun sequence.
ACCESSION   DATASH010000231 DATASH010000000
VERSION     DATASH010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 14212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14212
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1613156"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(238..1092)
                     /locus_tag="VHV99_06445"
     CDS             complement(238..1092)
                     /locus_tag="VHV99_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007744342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3635116.1"
                     /translation="MSSIAYRHADVDGFNVFYREAGRPNAPKLLLLHGFPSSSHMFRD
                     LIPLLAEQFHIVAPDLPGFGQSDMPARETFAYTFDNLANVIERFTEQIGFDRFAVYVF
                     DYGAPTGFRLALRHPERIAAIISQNGNAYEEGLSEGWNPIRAYWQDPSHANREALRAM
                     LAHETTVWQYTHGVQDTASVSPDGYSLDNFYLSRPGADEVQLDLFRDYRNNVALYPAF
                     QQYFRSHQPPFLAVWGKHDPFFLPAGAEAFKRDIPNAEVRFLDTGHFALETHAADIAA
                     AISGFLLR"
     gene            complement(1155..1862)
                     /locus_tag="VHV99_06450"
     CDS             complement(1155..1862)
                     /locus_tag="VHV99_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3635117.1"
                     /translation="MSIGHVSAVLVHGAWADGSSWSKVIQRLNARGIHAVAAPLPLSS
                     LPDDVAALDRTLERIEGPVVLAGHAYAGAVIGATRADNVKALVYVAALAPDEGETVGD
                     VFYRGAAHPLAPKLSPDRHGLIWLPDEAFATAFAQHAGAQELMVLGAIQRPISVSCIG
                     EAVGRPLWKDRPSWFVSAGEDRMINPDTQRFMAQRMNARVHSHEVDHTPMITAPDAVT
                     NVIVEAIETVSAHQEYP"
     gene            2040..2672
                     /locus_tag="VHV99_06455"
     CDS             2040..2672
                     /locus_tag="VHV99_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014252091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABATE domain-containing protein"
                     /protein_id="HEX3635118.1"
                     /translation="MDYRQIPAILLADAPGLDFLNSIATPVDEPVDWIRDGEGLLAWL
                     DQAGLAPRAALEGIRNRSTAAELDEVAARARELREWFREFVRERRGRPLLATDLRELE
                     PLNQLLEQDQQHGQIIANASEGATHFELRAQRHWQSAASLLMPIAEVLAKLVCEEDFT
                     YVKACEGPKCTLLFADHTRGHARRWCSMAICGNRAKVAAHRARLKEQLNG"
     gene            complement(2767..3543)
                     /locus_tag="VHV99_06460"
     CDS             complement(2767..3543)
                     /locus_tag="VHV99_06460"
                     /inference="COORDINATES: protein motif:HMM:NF012859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RICIN domain-containing protein"
                     /protein_id="HEX3635119.1"
                     /translation="MLIAFTSSLGAFSGLAKAQTAATIKLSGTNVCTDVNAESKTPGV
                     AVITWACNGGNNQNWTPTPTGTQYTFVNLNSNLCLDVAGISMTAGAAVDQWTCNGQPN
                     QRFTLNSQNNGFAIIAVHSGLCLAATSLTAQSPQITQQACSGAAVQTWQISGMSATTL
                     PSKWSAPVTLPLVPNCHTQTASTTAFAPPLTPGYLLDATSRRGGPRVAITRRPSAARS
                     GTASIRPTSCCASPCLATTFPTPSAPRFGVSLPTTNAETQ"
     gene            complement(3619..4203)
                     /locus_tag="VHV99_06465"
     CDS             complement(3619..4203)
                     /locus_tag="VHV99_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016347302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX3635120.1"
                     /translation="MTRRALLCDAALATLASVSLPACAQHGTTGLVSPPLRLDDTWLV
                     DQTGRKRLLSDLLLQRVTAVQTIYTGCSSVCPLQVALFSAVQARMLQIRARYPVQLLS
                     IGIDPLSDSPSALHEWLMRFQAGPSWTAATPTLRDVDQMRTRLSGSALPLGNLADHST
                     QIYCFDARAMLRWRSTDLPRADEVCDVLSALGRT"
     gene            4676..5650
                     /locus_tag="VHV99_06470"
     CDS             4676..5650
                     /locus_tag="VHV99_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3635121.1"
                     /translation="MNNFIRPPVFYPSSVVFVDDNDSYLDALRRFFPDISTNLFFTRP
                     QTALAFICKHARENSLEFAPASAGLSETGLERFVESSAERDILARDSRFSEVAAVVVD
                     YDMPGLSGVEFLSSISNLRCAKVLLTGVADETVAVKAFNAGIVDLYLRKTDADSAGRL
                     VHFLKDARKRHCSEAGWLGLGENGVTYCDPRTRKVLDEVVAAEGIVEYYWRPEQNAIL
                     MFDREGNPSVFLAWAENDWIAQAEIVADESGPVDLLRRMAVRESMPVFWPHLAFRPDA
                     DFTTLTPRTIPDWDDAFYCWTRIDAKDLGLNALTFARWRSDRNQHARG"
     gene            5724..7163
                     /locus_tag="VHV99_06475"
     CDS             5724..7163
                     /locus_tag="VHV99_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007747670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3635122.1"
                     /translation="MYAIPTAFVSALFVVFGLYVLITQGVTRLSAPFLLMCATTFAWQ
                     GTWTLLFQTENPHLASVLVRVGYLFILFLPTTFYHFVTEVAARRRERPLLLASYGVCL
                     VLAVLLPGSEVVAGYRHYFFGYYPSAGTLHPLHVLQTVLLAGRSGQLLLAARKQAGGE
                     ARRRLDLCFASLCLYSFAAVDYAVNYGYPLYPPGVLFIALSLGLLAITIVRYHLIHPY
                     AIAATIAHEIATPLATIAMHADEIASVWSHVFNGYQLAVTHGLYDDHEMQAGQAERIS
                     QLATSIRREVSGTSAMVEMALASFTLDRLDRTRFSPHSIQQCVTSALERYPFRGDERV
                     RVSVAPIDPALCFSGSDTLAVFVLFNLLKNALHAIHVSGEGEIAISAVPEQGFCVLRF
                     RDTGPGITPDILPHIFDAFFSTKRHGSGAGMGLAFCRRAAELLGGSIECSSTRGSHTT
                     FTVRLPVPGSVADRALKKMPARSLRRRQG"
     gene            complement(7269..7766)
                     /locus_tag="VHV99_06480"
     CDS             complement(7269..7766)
                     /locus_tag="VHV99_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018314448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX3635123.1"
                     /translation="MLMRDPIPNALNRGSALLSEDSLTPEPLKTFHHTFDIYLKDSNA
                     FMNTYFSRYFEWQGVCRERWFHECIHNDLLGSDGMFVTKRAHQEYVHETFPFQHVDCY
                     LNTVQVKQCSAYLLFRFCVDGREVSKGYQQILYAGRDRKIRRFPEGIVERVKEYELPL
                     TALSA"
     gene            8386..10644
                     /gene="katG"
                     /locus_tag="VHV99_06485"
     CDS             8386..10644
                     /gene="katG"
                     /locus_tag="VHV99_06485"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HEX3635124.1"
                     /translation="MSDEAKCPFNHAAGGGTTNRDWWPKQLRLDLLSQHSNKSNPLDN
                     NFNYAEAFKSLDLAAVKKDLAALMTNSQDWWPADFGHYGPLFIRMAWHSAGTYRIGDG
                     RGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKLSWADLLILTGNVALETM
                     GFKTFGFGGGREDTWEPDQDVYWGNEKTWLGGDVRYGKGAAGDNEDGGVIVADVEKHG
                     EEVSRDDNGRILENPLGAVQMGLIYVNPEGPDGNPDPLAAAHDIRETFARMAMNDEET
                     VALIAGGHTFGKTHGAGPADNVGPEPESADLENVGLGWKNSFGTGKGADTITSGLEVT
                     WTTAPTKWDNGFFENLFKHEWELTKSPAGANQWVARSTDETIPHAYDPSKKLRPTMLT
                     TDLALRLDPEYEKISRRFLENPDQLADAFARAWFKLTHRDMGPRARYLGPEVPAEELL
                     WQDPIPAVDHPLVAEQEIASLKQKILASGLSVSQLVSTAWASASTFRGSDKRGGANGA
                     RIRLAPQKDWAVNQPEQLAKVLKVLEGIQGEFNGTQSGKKVSLADLIVLAGSAGIEQA
                     AKNAGHQVSVPFTPGRMDASQEQTDVQSVGALEPIADGFRNYVKGKYSVPPEALLIDK
                     AQLLTLTAPEMTALIGGLRVLNVGTGQNPQGAFTHKPETLTNDFFRNVLDMGTEWKPA
                     SAAREVFEGRDRKSGELKWTGSRVDMVFGSHAQLRALGEVYASEDWQEKFVHDFVAAW
                     VKVMNLDRFELA"
     gene            complement(10823..11599)
                     /locus_tag="VHV99_06490"
     CDS             complement(10823..11599)
                     /locus_tag="VHV99_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3635125.1"
                     /translation="MRFTGKTALVTGGNSGIGFTAAKLLIAEGARVAITGRDRAKLDQ
                     AVSELGENALGIQADLDDETAIESMFKQIKETFGSLDIVFANAGISGPTPLGGTTAAA
                     FEAILRTNLTAVFLTVQAALPLMSAGGSIVLNGSVMRELGSPGSAAYSATKAGVTGMA
                     KVFASELVQRGIRVNTVIPGGTRTPIWTRGARAGATLDGTEEAFAPRIPMARLATPEE
                     VARAALFLASDDASGITAAEIVVDGGTIGAPWGAPMFRKG"
     gene            11814..12395
                     /locus_tag="VHV99_06495"
     CDS             11814..12395
                     /locus_tag="VHV99_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX3635126.1"
                     /translation="MGYSQAQKAESRQRVLENASRQIREHGIEALGVADCMRSAGLTH
                     GAFYGHFSSRDALILEALEHAVSQSEKRIASLATGAVKRGETPLQAIAEVFLNERHVK
                     NPGNGCALCALAGEARHANPDVRERLTSYVRKLASQIAQAASSDSTEAALGIVATIVG
                     AITLARAADDDKLAKSILSASLALVMTQEGGAR"
     gene            12477..13871
                     /gene="otsA"
                     /locus_tag="VHV99_06500"
     CDS             12477..13871
                     /gene="otsA"
                     /locus_tag="VHV99_06500"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase
                     (UDP-forming)"
                     /protein_id="HEX3635127.1"
                     /translation="MSRLVVVSNRTTDPRKPAAGGLAVALRESLQQTGGLWFGWSGKI
                     HEAADGAQAGQGEVHVQTAGNVTLATLDLSREDHDTYYLGYSNDVLWPVFHYRLDLAN
                     FDTRFAAGYRRVNQLFARKLLPLLKPDDLIWVHDYQLIPLAAELRAMGCTNRIGFFLH
                     IPMPPPLIMAAIPEHEWLMRSLFAYDLVGFQSEADLTHFSHYVESEAQAQRLSPDRLR
                     AFNRTLRVGAYPIGIDVDDFIRLADSKEGRDMFVRMRDEYSRRRLLLGVDRLDYSKGL
                     PQRVHAFRELLTYYPENRDSATLIQIASPSREDVGAYDDLRRQMDSLCGAINGDFGEL
                     DWMPVRYIHRNVARKRLPGLYRASRVALVTPLRDGMNLVAKEFIAAQDAADPGMLVLS
                     RFAGAAEQLKEALLVNPYDTQGTAQAIQRALTMSLDERINRHEALLDRIRKFDVHWWS
                     STYLQALEDCGAKE"
BASE COUNT         2840 a         4160 c         4441 g         2771 t
ORIGIN      
        1 tgtcacggag ctggttcaac ctggatgcaa tctgggcatc gagcctcatc ctggttggcc
       61 tgctctcgct gttgtgacgc gtcatgcaag gcttcgccga atgggaaatt gaagaatgtc
      121 cggctgagtg tcaagctatc aattgatcaa tcgacccgcg gcgttctatc tccccgactc
      181 gtgcatgagc cgcatgaaca tcgattgagt ggacaacaac cttcctgcgt ttccaggtca
      241 gcgaagcaga aatccggaaa tggccgcggc gatgtctgcc gcatgcgttt ccagggcgaa
      301 gtgccccgta tcgagaaaac gcacctcggc gttcgggatg tcgcgcttga atgcctcggc
      361 gccggccggc aggaaaaacg ggtcgtgctt gccccacacg gcaaggaaag ggggctggtg
      421 gctgcggaaa tactgctgga atgcgggata caacgcgacg ttgttccggt agtcgcggaa
      481 cagatcgagc tggacttcgt ccgcgcctgg gcggctcaga tagaagttgt cgagcgagta
      541 gccgtcgggg gagacggaag ccgtgtcctg cacgccatgg gtgtactgcc acaccgtcgt
      601 ttcgtgcgcc agcatcgcgc gcagtgcctc gcgattggcg tgggaaggat cttgccaata
      661 agcgcggatc ggattccagc cctcgctcag gccttcttcg tatgcgttgc cattctgcga
      721 gatgatcgcg gcgatccgtt cgggatgcct gagagcaagc cggaagcccg tcggcgcacc
      781 atagtcgaac acgtacacag cgaagcgatc gaaaccgatc tgttcggtga agcgttcgat
      841 cacgttggcg aggttgtcga acgtgtaggc aaacgtctcg cgagccggca tgtccgattg
      901 accaaaaccg ggcagatcgg gcgcgacgat atgaaactgt tccgcaagca gcgggatcag
      961 gtcgcgaaac atgtgactcg agctcggaaa gccgtgcagg agcagcagct tcggcgcgtt
     1021 cggccgaccc gcctcgcgat agaacacatt gaagccgtct acatcagcgt gacgataggc
     1081 aatagaggac atggttcgac tcctgattgg gtgaatggcg tgctgaaact ggcaggcgta
     1141 tgccagtacg acagttacgg atattcctgg tgagccgaga ccgtttcgat cgcttcgacg
     1201 atcacgttgg tgacggcgtc tggggcagtg atcatgggtg tgtgatcgac ttcgtgtgaa
     1261 tgcacgcgcg cgttcatgcg ctgtgccatg aaacgttgcg tgtcgggatt gatcatccgg
     1321 tcctcgccgg cgctcacgaa ccagctcggc cggtccttcc agagtggacg tccaactgcc
     1381 tcgccaatgc acgaaaccga aatgggacgt tgaatcgcac cgagcaccat cagctcctgc
     1441 gcgccggcgt gctgcgcgaa cgccgtcgcg aatgcttcgt cgggcagcca gatgaggccg
     1501 tgccgatccg gtgacagttt gggcgccaac gggtgcgcgg cgccgcggta gaacacatcg
     1561 ccgacggttt cgccttcgtc aggcgcaagc gcggccacgt agaccagcgc cttgacgttg
     1621 tcggcgcgcg tcgcgccgat cacagcgccg gcgtaggcat gccctgcaag cacaaccggg
     1681 ccttcgatgc gttccagcgt tctatcgagc gcggccacgt catccggcag ggaactgagc
     1741 ggcagcggcg ctgcaacggc atgaatgccg cgtgcattca gacgttggat gaccttgctc
     1801 cagctcgaac catctgccca cgcgccgtgc accagcaccg cactcacatg accaatcgac
     1861 atttttcgct cctttgacgg aattaggcgc tgcttgtcag gcactcacgt cgtgtttgcg
     1921 tcgcgtgttt cgtaacctga ttaatgttaa tttacaggtt acaaactagc cgagtaactt
     1981 gtcaaggtca tttttagtgg ttacaatgct ttcattgtca cttcagggcg cgatgaaaga
     2041 tggactaccg tcaaatccct gcgatattgc tggcggacgc gccaggcctc gatttcctca
     2101 actcgatcgc aaccccggtg gatgaaccgg tggactggat ccgcgacggg gaaggtttgc
     2161 tggcgtggct ggatcaggcg gggctcgcgc cgcgcgcggc actcgaaggc atacgaaacc
     2221 ggtcgacggc agccgaactc gacgaggttg cggcacgcgc gcgcgaattg cgcgagtggt
     2281 tcagggaatt tgtccgggag aggagggggc ggccgctgct tgccacggat ctacgcgaac
     2341 tcgaaccgtt gaaccaattg ctcgagcagg atcagcagca cggccagatc atcgcgaacg
     2401 cttcggaggg ggccacgcat ttcgagcttc gcgcgcagcg gcactggcag tcggcagcgt
     2461 cgctattaat gcccatcgcc gaggtattgg cgaagctggt atgcgaagag gatttcacgt
     2521 acgtgaaagc gtgtgaaggc cccaaatgca cgttgttatt cgcggatcac acgcgcggtc
     2581 acgcccgtcg gtggtgcagc atggcaatct gcggtaatcg cgcgaaggtc gcggcgcacc
     2641 gcgcgcggct caaggagcag ctgaacggtt aggcgagcct cgacgcggat cgcctcgggg
     2701 gcgagcaagg tccgccacgc aagcgccgtg caacggctgc cgaacgggtg gggaaaacgg
     2761 ctcgcttcat tgcgtctctg cgttagtcgt tggtaatgaa acgccaaagc gcggcgcact
     2821 cggtgtcgga aaggtcgtag cgaggcatgg acttgcgcaa catgacgttg gccggatcga
     2881 tgccgtccct gagcgcgcgg cagagggtcg ccgggttata gcgactcggg ggccgccacg
     2941 gcggcttgtt gcatcgagca gatagccggg cgtcaacggc ggggcgaagg cggtcgtcga
     3001 cgcggtttgc gtgtgacagt tgggaacgag tggcaaggtg accggcgccg accacttcga
     3061 gggcagcgtc gtcgcactca tcccgctgat ctgccaggtc tgcacggcag ccccgctaca
     3121 ggcctgctgc gtgatctgcg ggctttgtgc ggtgaggctc gtcgcagcca ggcacaggcc
     3181 gctgtgcacc gcgatgatcg caaagccatt attttgcgag ttcagcgtga atctctggtt
     3241 gggctggccg ttgcaggtcc attgatcgac ggccgcgccg gcagtcatgc taatgcccgc
     3301 cacgtccagg catagattgc tgttcagatt gacgaatgtg tattgggttc ctgtcggtgt
     3361 gggcgtccaa ttctgattgt tgccgccgtt gcaggcccag gtaatcaccg cgacgcccgg
     3421 cgttttcgat tccgcgttga catccgtgca cacattcgta ccgctcagct tgatcgtggc
     3481 tgccgtttgc gctttagcga gtccggaaaa tgcgccgagg ctcgacgtga aggcgatcag
     3541 caacaggact ttgccgcacg ggagaagccc ttggtgaaac ggattggtgc catgacctta
     3601 attccggttg agggcgaact aggtgcgccc tagcgcactg agtacgtcgc agacttcatc
     3661 cgcgcgcggc aggtcggtac tgcgccagcg caacatggcg cgcgcatcga agcaatagat
     3721 ctgggtggaa tgatcggcga ggttgccgag cggcagcgca ctccccgaaa gccttgtgcg
     3781 catctggtcg acgtcgcgca acgtcggcgt ggccgcagtc cagctgggac cggcctgaaa
     3841 gcgcataagc cactcgtgta aagcggaggg cgagtcggaa agcggatcga ttccgatcga
     3901 cagcaattga accggatacc gagcccggat ttgcagcatg cgcgcctgaa ccgcgctgaa
     3961 cagggcaacc tgcagaggac acactgaact gcacccggtg tagatggttt gcactgcagt
     4021 gacgcgctgc agcagcaagt cgctcagcag tcgcttcctg ccggtttgat cgactaacca
     4081 ggtgtcgtcg agacgaagcg gggggctcac gagtcccgtc gttccgtgct gggcgcaggc
     4141 gggcaatgac accgaggcca acgtggccag agccgcatcg cacagcaagg cacgccgggt
     4201 caatcgcctg agcgacgccg gttgggccgg tgccaggcgc gtgcgaactt ttcgagggaa
     4261 caccccttct tctccgcaag aatctgatga actcacccat cacgcgcagc cgagaatgcg
     4321 cggtcgcgcg acagtcggtg aatcaggcgc gagataggat taatgagctc gtctgctcgc
     4381 tctcgtgtgt gtgatcaggc gatacagaca agcgtaggtg ccgaatgcga ttctacggac
     4441 gcgagttggt cgcgattgtg ctggcgcaca tttctcgtgg ggcgtcgcga gaacacggtt
     4501 tgattgagag aaattattcc atttgcatac cgtgacgcat acgtagcgca agtttgcacg
     4561 ttcatgtgaa gtccccggta ctatatgggg cagcgggaat tggcccggga gccagcgtga
     4621 gagaaaccgg cgtgtcagcc ggtacaaggc gcgccgcata ttccacgtaa aaaacatgaa
     4681 caactttatt cgccccccag tcttctatcc gtcatctgtc gttttcgtgg atgacaacga
     4741 cagctacctc gatgcgttgc gccgattttt tccggatatt tccaccaatc tgtttttcac
     4801 gcgacctcaa accgcactcg ctttcatctg caaacatgcc cgcgagaatt cgctggagtt
     4861 cgcgccggcg tcggccggtc tgagcgagac gggcctcgag cgctttgtcg aaagctcggc
     4921 ggagcgagac atactggcgc gcgattcgcg attttcggag gtagccgcgg ttgtcgtcga
     4981 ttacgacatg ccgggactga gcggcgtcga attcctttcg tccatttcca atttgcggtg
     5041 cgcgaaggtg ctgttgaccg gcgtcgcgga tgaaaccgtc gcggtcaagg ctttcaatgc
     5101 cggcatcgtc gatctgtatc tgcgaaagac ggatgcggat tcagccggca gactggttca
     5161 ttttctgaaa gatgccagaa agcggcattg ttcagaggcg ggctggcttg gcctgggcga
     5221 gaatggcgtc acctattgcg acccgagaac gagaaaggtt ctcgacgaag tggtggccgc
     5281 agaaggcatt gtcgagtact actggcgtcc ggagcagaat gccattctga tgttcgaccg
     5341 tgagggcaac ccgagcgttt tccttgcgtg ggctgagaac gactggatcg cccaggcgga
     5401 gatcgtggcg gatgagagcg ggccggtgga tctgttgagg cgcatggcgg tacgcgagtc
     5461 gatgcccgtt ttctggccgc acctggcgtt tcgtccggac gcggatttca cgacgctgac
     5521 accccgaacc atccccgact gggacgacgc tttctattgc tggacccgaa tagatgcgaa
     5581 agacctgggt ttgaacgcac tgacatttgc gcgatggcgg agcgaccgga atcagcacgc
     5641 ccgtggatga cgggttggac ccttcgcgta gtccgctatg aatatgccgg gatagaccaa
     5701 cgtttccaga cccatcgagc cacatgtacg ccattccgac tgcctttgtg tccgcgctgt
     5761 tcgtggtgtt cggcctttac gtactgatta cacaaggcgt gacgcgtctg tcagcgccat
     5821 ttcttttgat gtgcgcgacg acattcgcgt ggcagggcac gtggaccttg ctgttccaga
     5881 ccgaaaaccc ccatcttgca agcgtgctgg tccgcgtcgg ttatctcttc attctctttc
     5941 tgcccaccac gttctatcac ttcgtcaccg aggtggcggc aagacgccgt gaacggccgt
     6001 tattgctcgc gtcttacggc gtgtgtcttg tgctggcggt attgctgccc ggcagcgaag
     6061 tggttgccgg ctaccggcac tacttttttg gctactatcc gagcgccggg acgctgcacc
     6121 ccttgcacgt attgcagacc gttttgctcg ccggccgcag cggacagctt ctgctcgcgg
     6181 cccgcaaaca ggcgggtggc gaggcgcgca ggcgcctcga cctctgcttt gcgagccttt
     6241 gcctgtattc gtttgcagcg gtcgactatg cggtcaacta tggctacccg ctctatccgc
     6301 cgggcgtgtt gttcatcgcc ctcagcctgg ggctgctcgc gatcacgatc gtccgctatc
     6361 acctgattca cccctatgcg atcgcggcga ccatcgcgca cgagatcgcg acaccgctcg
     6421 cgacgatcgc catgcacgcc gatgaaatcg ccagcgtctg gtcccacgtg ttcaacggtt
     6481 accagctagc cgtcacacat gggctttacg acgatcatga gatgcaagcc gggcaggccg
     6541 aacgcattag ccagctcgcc acatcgatca gacgggaggt gtcgggcacg agcgcaatgg
     6601 tcgagatggc gctcgcatcg ttcaccctcg atcgactgga ccgcacccgc ttcagcccgc
     6661 attccatcca gcaatgtgtg acgtcggcgc tggaacgcta tccgttccgc ggcgacgagc
     6721 gggtgcgcgt tagcgtggcg ccgatcgacc cggccctctg cttttcaggc tcggacacgc
     6781 tggcggtttt cgtcctgttc aatctgctga aaaacgcatt gcacgccatt catgtgagcg
     6841 gcgagggcga gattgcgatc agtgcggtgc cggagcaggg gttttgcgta ctgcgattcc
     6901 gcgacaccgg cccgggcatc acgcccgaca tcctgccgca tatcttcgac gccttcttct
     6961 cgaccaagcg ccacggaagc ggcgcgggaa tggggctggc attttgccgc cgcgccgcgg
     7021 agcttctggg cggcagtatc gaatgcagtt cgacgcgtgg ttcccacacc acgttcaccg
     7081 tgcgcttgcc ggtaccgggc tcggtggcgg accgcgcact gaaaaaaatg ccggcgcgtt
     7141 ccttgcgccg gcgtcagggg tgaaggttaa ggttggaagt tagtgttgaa accccctagt
     7201 aaaactcaac gagaaaacag cgagaaaagc cagcgataaa acttgacggc gaaagtcatt
     7261 cgtcacgatc aggcgcttag cgccgtcaac ggcagctcat attccttgac ccgttcgacg
     7321 ataccttccg ggaaccgcct gatcttgcgg tcccgtccgg cgtacaggat ctgctgataa
     7381 cccttcgaga cctcgcgccc atccacgcag aagcgaaaca gcaggtaggc cgagcactgc
     7441 ttgacctgca ccgtgttgag atagcaatcc acgtgctgga aagggaaggt ttcgtgaaca
     7501 tattcctggt gcgcgcgttt ggtgacgaac atgccgtccg atcccagcaa gtcgttgtgt
     7561 atgcactcat gaaaccaccg ctcgcggcat acaccttgcc actcgaaata gcgcgagaaa
     7621 tatgtattca tgaacgcgtt cgagtccttg agataaatgt cgaacgtatg gtggaaagtc
     7681 tttagcggct ccggggtcaa cgagtcttcg gaaagaaggg cggatccgcg attcagggcg
     7741 ttagggatgg ggtcacgcat caacatacga atttcctttt gcaggaatgg ctgctcgcgg
     7801 caaccacgca tgccttcctg tatggcaggc acatcccatt ctgttgggat gtgggccgca
     7861 tggctattgg ctgacaaaca cgccacgcag aaatcctgga cgacagcttg cataagagcg
     7921 acatgttcag atgggcaaat gttgctgcag tgcaagaacg aacggaaaca tcgctgctcg
     7981 atgcgcgccg catgtgtagc cgattgcgct ggcacgacgg tatgtccctc atgcgtacgt
     8041 gaaccgccgc tgtgtggtgc gccgaacggt gcgccagaag cgcttgccac gccatagtca
     8101 tggtctatca ctggcattgc cacaatccca tactattggc ggccactctc acgtatattt
     8161 tgaagtgcag cagcctgccg acgcgagcca tcctcgtcca atgcgcgcca cctgatccgt
     8221 cacggacgac gaccatgcac gtcgggtgat caacagtggt acaccagaaa cgcccattat
     8281 gtgatttccc ggcagccccg ccggcgtaag tcggcgatgc accatacata gaattcagga
     8341 gccgtagcac aacaagtagt gggtacgtcc tcaaggagat gacgcatgtc agacgaagca
     8401 aaatgcccgt ttaaccacgc cgccggcggc ggcacgacca atcgtgactg gtggccgaag
     8461 caactgcggc tcgatctgct cagccagcac tccaacaagt ccaatccatt ggacaacaac
     8521 ttcaactacg ccgaggcgtt caaaagtctc gatctcgctg cggtgaaaaa agacctcgca
     8581 gccctcatga ccaattcgca ggattggtgg ccggcggact tcggccacta cggtccgctc
     8641 ttcatccgca tggcctggca cagtgccggc acctaccgca tcggcgacgg ccgcggtggc
     8701 gcggggcgcg gccagcagcg tttcgcgccg ctcaacagct ggccggacaa cgtcagtctc
     8761 gacaaggcgc gccgactgct ctggccgatc aagcagaagt acggccagaa actttcgtgg
     8821 gccgacctgc tgatcctcac cggcaacgtc gcgctcgaaa ccatgggctt caagaccttc
     8881 ggcttcggcg gcggtcgcga ggacacatgg gaaccggacc aggacgtcta ctggggcaat
     8941 gaaaagacgt ggttaggcgg cgacgtccgc tatggcaaag gagccgcagg cgataacgag
     9001 gacggcggcg tgatcgttgc cgacgtagaa aaacacggtg aagaggtgag ccgcgacgac
     9061 aacggacgga ttctggagaa tccgctgggc gccgtgcaga tggggctgat ctatgtgaac
     9121 ccggaaggtc ccgacggcaa tcccgatccg ctggccgcgg cacacgacat ccgcgaaacc
     9181 ttcgcgcgca tggccatgaa cgacgaggaa accgtcgcgc tgatcgccgg tggtcacacc
     9241 ttcggcaaga cgcacggtgc cggtcctgcc gacaacgtcg gtcccgaacc cgagtccgcc
     9301 gatctcgaga acgtggggct gggctggaag aacagcttcg gcaccggcaa gggcgccgac
     9361 acgatcacca gcggtctgga agtgacctgg accaccgcgc cgacgaagtg ggacaacggc
     9421 ttcttcgaga acctgttcaa gcacgagtgg gaactgacca agagccccgc cggtgccaat
     9481 cagtgggttg ccaggagcac cgacgaaacc atcccgcacg cttacgaccc gtcgaaaaag
     9541 ctgcggccga caatgctcac caccgacctc gcactacgcc tggaccccga gtacgagaag
     9601 atttcgcggc gctttctgga gaatcccgac cagctcgccg atgccttcgc ccgtgcctgg
     9661 ttcaagctga cgcaccgtga catgggaccg cgcgcccgct acctgggccc ggaagtgccc
     9721 gccgaagaac tgctctggca agaccccatt ccggcggtcg accacccgct agtcgctgag
     9781 caggagatcg cctcgctcaa gcagaagatt ctggcatcag ggctgtctgt ctcgcagttg
     9841 gtgtcgaccg cgtgggcgtc ggcatccacc ttccgcggtt cggacaagcg cggcggcgcc
     9901 aacggcgcac gcattcgcct cgctccccag aaggactggg cagtcaacca gcccgagcag
     9961 ctggcgaagg tcctgaaggt cctcgaaggc attcagggtg agttcaacgg cacgcagtcc
    10021 ggcaagaagg tgtcgctggc cgatctgatc gtgctggccg gcagcgccgg catcgagcag
    10081 gcggcgaaaa acgccggcca tcaggtgagc gtgcctttca cgcccggccg catggacgcc
    10141 tcgcaggaac agaccgacgt gcaatccgtc ggggccctcg aacccatcgc ggatggcttc
    10201 cggaactacg tcaaaggcaa atacagcgtg ccgccggaag ctttgttgat cgacaaggcg
    10261 caattactga ccctgaccgc ccctgagatg accgcgctca tcggcggctt gcgcgtgctg
    10321 aacgtaggga ccgggcagaa tccgcaaggc gccttcaccc acaagccgga aacgctgacc
    10381 aacgacttct tccgcaacgt gctcgacatg ggcacggaat ggaagccggc ttcggcggct
    10441 cgcgaggtgt tcgaagggcg tgaccgcaag agtggcgagt tgaagtggac cggcagccgc
    10501 gtcgatatgg tcttcggttc gcatgctcag ttgcgtgccc tgggcgaagt ctatgcaagc
    10561 gaggattggc aggaaaaatt tgtccatgac ttcgtggcgg cctgggtcaa ggtgatgaac
    10621 ctcgatcggt tcgaactagc ctgatcggcg cggggaaccc gccagggctg ttgccctggc
    10681 ggggttttat ctcgccgtgt gaaaagtgag cttgcaggtc gcgatggggc acacgccttt
    10741 cccgtgtcat ggcgagagct ttcctgaaag tcatcacgca tgcgctgatg ccaggttaag
    10801 gatgtcctcg agaagcggcc cttcacccct tgcggaacat aggcgctccc cacggcgcac
    10861 cgatcgtgcc gccgtcgacg acaatttccg ccgcggtgat tcccgaggcg tcgtcggacg
    10921 cgaggaaaag cgcagccctg gccacttctt ctggggtcgc aaggcgtgcc atcggaatgc
    10981 gcggcgcgaa tgcctcttcg gtcccgtcga gcgtggcgcc ggcccgggcg ccgcgcgtcc
    11041 agatcggcgt gcgcgtgccg cctggaatca cggtattgac gcgaatgcca cgctgcacga
    11101 gttcggatgc aaataccttg gccatgccgg tgacgcctgc cttcgtggcg gaataagcag
    11161 ccgacccagg cgagcccagt tcgcgcatca cggagccatt caatacgatc gagccgccgg
    11221 cgctcatcag cggaagcgcc gcttgaaccg tgagaaagac agcggtgaga ttggtgcgaa
    11281 gaatcgcctc aaatgcagcc gcggtcgtgc cgccgagcgg cgtcggaccg gagattccgg
    11341 cgttggcgaa cacgatgtcg agcgagccga atgtttcctt gatctgcttg aacattgact
    11401 cgatggcggt ttcatcgtcg agatcggctt gaatgccaag cgcgttttcg cccagctcgg
    11461 atactgcctg atccagtttc gcgcgatcgc ggccggtgat cgcaacgcgg gcgccttcag
    11521 caatcaaaag cttcgctgcg gtaaagccaa tgccgctgtt gccgccagtg acgagcgctg
    11581 tctttccagt aaatcgcatt tccttctcct gtgtattacg ccgcgagatc gcagcgaatg
    11641 ccgcgcctgc tggtggcgaa aaacctcgca aaaaacctgt taagcagcgc agaacagccg
    11701 ctgcatccaa cccggcatga gcgtcgtcaa catctcattc tgtatgttga tcatcatgca
    11761 gaaattcagt attattctcg tcatgcagat tgtcaagggt agcgaggtaa aaaatgggtt
    11821 actcgcaggc acaaaaggcg gagagcaggc agcgtgttct ggagaacgcg tcgcggcaga
    11881 ttcgcgaaca cggcatcgag gcgctcggcg tcgccgactg catgcgcagc gcggggctca
    11941 cgcatggcgc gttctacgga catttttcat cacgcgatgc gttgattctg gaagcgctcg
    12001 agcacgcggt gagtcagagc gagaagcgca tagcgtctct cgcaaccggt gcggtaaagc
    12061 gcggggaaac gcctctgcag gctatcgccg aggttttcct caacgagcgt cacgtgaaga
    12121 atccgggcaa tggatgtgcg ctgtgcgcac tcgccggaga agcgcggcat gcgaatcctg
    12181 acgtcaggga gcggcttacc agctacgtgc gcaagctggc gtcgcaaata gcgcaggccg
    12241 catccagcga tagcacagaa gctgcgctgg gtatcgtcgc gactatcgtg ggagccatta
    12301 cgctggcgcg cgccgcggac gacgacaaac tggcgaaatc gattctctcc gcgtcgctcg
    12361 cgctggtcat gacgcaggag ggcggggcgc gctagccttg ccgtgcccta accgcaacga
    12421 cagagtaaac tgggtcgatc agcacgtgat tgtgagcgca acaacacaag gacctcatga
    12481 gtcgactcgt cgtcgtttcg aatcgcacta cagacccgcg aaaacctgca gccggagggc
    12541 tcgccgtagc gttacgcgag agcttgcagc agacaggtgg actctggttc ggctggagcg
    12601 gcaagatcca cgaagcggcg gatggcgcgc aggccgggca gggcgaagtt catgtccaaa
    12661 ctgccggcaa tgtcacgctt gcgacactcg atctgagccg cgaggatcac gacacgtatt
    12721 acctgggtta ctccaatgat gtcttgtggc cggtattcca ctaccggcta gacctggcta
    12781 atttcgacac gcgattcgcc gcgggctatc gcagggtcaa ccagctattc gcgcgcaagt
    12841 tgctaccgct attgaagccg gacgatctca tctgggttca cgactaccag ttgatccccc
    12901 tcgccgccga gttgcgcgcc atgggatgca ccaatcgcat tggtttcttc ctgcatattc
    12961 cgatgccgcc gccgctgatc atggcagcga ttccggaaca cgaatggctg atgcgttcgc
    13021 tcttcgcata cgatctggtg ggcttccaga gcgaagccga cctgacgcat ttttctcact
    13081 acgttgaatc tgaggcacag gcgcagcggt tgagtccgga tcgccttcgg gcgttcaatc
    13141 gaacgctgcg ggtcggcgcg tatcccatcg gcatcgacgt cgacgacttc attcgtcttg
    13201 ccgactcgaa ggaagggcgc gacatgtttg tccggatgcg tgacgaatat tcgcggcggc
    13261 gtttgctgct gggcgtcgac cggttggact attcgaaggg gctgccgcaa cgggttcatg
    13321 cattccgtga actgctgact tactacccgg agaaccgcga tagcgcgacg ctcatccaga
    13381 ttgcgtcgcc gagtcgcgag gacgttggcg cttatgacga tctgcggcgc cagatggata
    13441 gcctgtgtgg cgcgatcaac ggcgacttcg gcgagctgga ctggatgccg gtgcgttaca
    13501 ttcatcgaaa cgtggcccgt aagcgcctgc cgggcctcta tcgcgcgagt cgcgtggcgc
    13561 tcgtcacgcc tttgcgtgac gggatgaatc tggtggcgaa ggaattcatc gccgcacagg
    13621 acgctgccga ccccggcatg ctggtgctct cgcgctttgc cggcgccgcc gagcaattga
    13681 aggaagcgct gctggtcaat ccctacgata cgcaaggcac ggcgcaggcc attcagcggg
    13741 cgctgacgat gtcgctcgat gagcggatca accggcatga agcgctgctt gaccggatcc
    13801 gcaagttcga cgtgcactgg tggagttcga cgtatctgca agcgttggag gattgcgggg
    13861 cgaaggaata ggtggtggat ggggacgcgg aacatttgta tgaagcaacg cccacagggc
    13921 gacgtggcaa ttctcagata tttgatttga cataatctta attatcaata ttcacggtgt
    13981 cagaactttt tgataatgtc cgattctggc tcattagaaa tgtccggttt cactccctga
    14041 gccacgcact gcgcgcatgc tgcgccatgg tggttgccca gggagccgga catgaacgca
    14101 cgtggattca tcacgatcag catgcacgag cttgagcggg tcaagatcat tgaggcggtt
    14161 gtcgaacacc ggctgaaggt ttttcaggcg gccgggcgtc tgcagctgtg cg
//
LOCUS       DATASH010000232         6445 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3590046,
            whole genome shotgun sequence.
ACCESSION   DATASH010000232 DATASH010000000
VERSION     DATASH010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6445
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3590046"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..4199
                     /gene="purL"
                     /locus_tag="VHV99_06505"
                     /gene_synonym="purI"
     CDS             84..4199
                     /gene="purL"
                     /locus_tag="VHV99_06505"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012213195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEX3635128.1"
                     /translation="MAHFSCFPGASALSDFRQTRLLETLTRIDANITGVRGQYLHFVN
                     AQTPLSAEDTAKIEALMHYGDPLEETKERGTAETFLVVPRFGTVSPWASKATDIAHLC
                     GLTQVRRIERGVEYTVTLKSGLLGGKKALSDEARAAVAAALHDRMTESVSPSRDHALH
                     LFDELPAKPLQTVDVLGNGRGALEAANTELGLALADDEIDYLVDAFTKLGRNPTDVEL
                     MMFAQANSEHCRHKIFNAEWTIDGEKQDISLFNMIRNTEKLNPQGTIVAYSDNSAIMA
                     GGVAERWFPRTPAEPGAMPEHYRRNVELTHTLMKVETHNHPTAISPFPGASTGAGGEI
                     RDEGATGRGARPKAGLAGFTVSNLELPDGVEAWENARDAAQPLAHRNPDDKHEAYGRP
                     DRIASPLQIMIDGPLGGAAFNNEFGRPNLGGYFRAYEQNVAGLVRGYHKPIMIAGGIG
                     NISDQHTHKHDLPEGSLLIQIGGPGMRIGMGGGAASSMATGTNTAELDFDSVQRGNPE
                     IERRAQEVINACWQLGEKNPILSIHDVGAGGLSNAFTEVVDGAGKGAIFDLRKIQLEE
                     SGLSPREIWSNEAQERYVLAIAPADLPAFEAMCERERCPFAVVGKATAERQLKVLDSE
                     SNHDVAHEPVDMPMEVLLGKAPRMHRDVKRVEQKLEPVDVTGIALSEVAVSVLRHPTV
                     ASKSFLITIGDRSVGGTTARDQMVGPWQVPVADVAITTMDYAGFRGEAMTMAERTPLA
                     VINAPASGRMAVGEAVTNIAAAPIASLDKLKLSANWMAACGAAGEDAALYDTVKAIGM
                     ELCPALGISIPVGKDSLSMRTKWEDRGVAKEVVAPVSLIISAFAPVEDVRRHLTPQLR
                     RASGTDGVGDSVLIAIDLGRGKQRLGGSILAQVTQQVGDTVPDVDDAQDLKRFFTAIQ
                     ALNSDGKLLAYHDRSDGGLWATVCEMAFAGHVGVSLNVDMLVLDPNHESDYGDAKDWA
                     KQTSGRREDRTIRALFNEELGAVIQVRAADRDAVLGALREQGLSACSHVIGKTNERDT
                     IEIYRDAKKIYEAPRTELHRTWSEVSWRISRLRDNPACADAEYDALSDAADPGISPVL
                     TFDPTEDVAAPFIAQGDFLRGVGKGARPRVAILREQGVNSHLETAYAFDRAGFDAHDV
                     HMSDLLAGRANLADFAGAVACGGFSYGDTLGAGEGWAKAIRFNSQLADMFAAFFGRED
                     TFALGICNGCQMMSSLASMIPGAEAWPKFTRNKSEKFEARFSLVEVQASPSIFFAGME
                     GSRIPVAIAHGEGYADFSQQGDASKVAVAMRFVDHRGQATEQYPFNPNGSPNGITSVT
                     TPDGRFSVLMPHTERVHRAVQMSWHPEGWGEGSTDASPWLRVFQNARRWLG"
     gene            4440..4727
                     /locus_tag="VHV99_06510"
     CDS             4440..4727
                     /locus_tag="VHV99_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3635129.1"
                     /translation="MRQSDVFISNMTVLGDLLRASRLAQGLTRDDVAIATGLSPKFIT
                     HIEAGKPTAQIGKVLHLLGELGIALRAQVSVDIPPAFATQPRHRRRVTHGS"
     gene            4717..6009
                     /locus_tag="VHV99_06515"
     CDS             4717..6009
                     /locus_tag="VHV99_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HEX3635130.1"
                     /translation="MAARTLVAYANGQRVGAVSDEGGIWSFTYDKAWLDREDAFVLSP
                     AFPLHAEPFVDGSSQRPVQWFFDNLLPEEEMRTALAREAKVNASDAWGLLAYYGRESA
                     GALTLLAEGEQEASGGLQPLSYADLEARIQAMPQRALIATAPKRMSAAGAQQKLLLSL
                     QGEAPNDRLFEPVGHEPSMHLLKPDMRGTGYPHSAINEFFCMQLARAMGVRVPPTHFM
                     RVPSACYVIDRFDRDTAAEPVRRLHTVDAMQLSNYDRNFKYQNATAQVLRDAIEKTGT
                     RAMARLDVFRWAVFNVLIGNADSHLKNLSFFVNARGYRLAPFYDLVSTVVYHTPTYQP
                     QQRDRWPQCDLTMPIGAATQFADVSRQNMLAFGEALGLAPKGSEKLLDEMLAVVGTRV
                     ADTRRAVGEVAKPNAGEVRLLDSIVAMPLAEMSRALRK"
     gene            complement(6191..>6445)
                     /locus_tag="VHV99_06520"
     CDS             complement(6191..>6445)
                     /locus_tag="VHV99_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015601097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX3635131.1"
                     /translation="GDLDWSDPKAYVPEFADAATHLQKGQMTDTPVHTQFGWHIIRVD
                     DVRNVTPPPLEQVRPQIVQQIQQEKLQAFEEGLRKNAKIQ"
BASE COUNT         1234 a         2021 c         2058 g         1132 t
ORIGIN      
        1 acttcctttg cgcctcacgt cgccttcacg cgcgactcat cggcatcact catcggcatc
       61 attagcgacg caacgtcaag tccatggccc acttctcgtg tttccccggc gcttcggccc
      121 tttccgattt ccgtcaaacc cgcctgctcg aaacgcttac gcgcatcgac gccaacatca
      181 ccggcgtgcg cggccagtat ctgcacttcg tcaacgcgca aaccccgctt tccgcggaag
      241 acaccgcgaa gatcgaagcg ctgatgcatt acggcgatcc gctcgaagag accaaagagc
      301 gcggcacggc cgaaactttc ctggtggtgc cgcgtttcgg cacggtgtcg ccgtgggcca
      361 gcaaggcaac cgatattgcc catctgtgcg gtttgacgca ggttcggcga atcgaacgtg
      421 gcgtcgaata cacggtcacg ctgaagagcg gcctgctggg cggcaagaag gcgctctccg
      481 acgaagcccg ggcggccgtc gccgcggcgc tgcacgaccg catgaccgaa agtgtttcgc
      541 cctcgcggga ccacgcgttg catctgttcg acgaactgcc ggccaagccg ctgcaaaccg
      601 tcgacgtgct cggcaacggc cgcggcgcgc tggaagcggc gaacacggaa ctgggcctcg
      661 cgctcgccga cgacgaaatc gactacctgg tggacgcatt caccaaactc ggccgcaacc
      721 cgaccgacgt cgaactgatg atgttcgcgc aggccaacag cgagcactgc cgccacaaga
      781 ttttcaacgc ggaatggacc atcgacggcg agaagcagga catctcgctg ttcaacatga
      841 tccgcaacac cgagaagctg aatccgcagg gaacgatcgt cgcgtattcg gacaactcgg
      901 cgatcatggc gggcggcgtg gccgagcgct ggttcccgcg cacgccggct gaacccggcg
      961 cgatgcctga gcactaccgc cgcaacgtcg aattgacgca caccttgatg aaggtggaaa
     1021 cgcataacca cccgaccgcg atctcgccat tcccgggcgc ctcgaccggc gcgggcggcg
     1081 aaatccgcga cgaaggcgcg accgggcgcg gcgcgcgtcc gaaggcgggt ctggcgggct
     1141 tcacggtgtc gaatctggaa ttgccggacg gcgtcgaagc atgggagaac gcccgcgatg
     1201 cagcgcagcc gctcgcccac cgcaatccgg acgacaagca cgaagcgtac ggccgtcccg
     1261 accgcatcgc gtcgccgctg caaatcatga tcgacggccc gctcggcggc gccgcgttca
     1321 acaatgaatt cggccggccg aatctgggcg gctatttccg tgcgtatgag cagaacgtcg
     1381 cgggtctggt gcgcggctat cacaagccga tcatgatcgc cggcggcatc ggcaatattt
     1441 ccgatcagca cacgcataag cacgatctgc cggaaggctc gctgctgatc cagatcggcg
     1501 gtccgggcat gcgcatcggc atgggcggtg gtgcggccag ctcgatggcg actggcacga
     1561 acacggctga actcgacttc gactcggtcc agcgcggcaa cccggaaatc gagcgtcgcg
     1621 cgcaggaagt catcaatgcg tgctggcaac tcggcgagaa aaacccgatt ctgagcattc
     1681 acgacgtcgg cgcgggcggc ttgtcgaacg cgttcacgga agtggtggac ggtgcgggca
     1741 agggcgcgat cttcgatctg cgcaagatcc agctggaaga gagcggtttg tcgccgcgcg
     1801 aaatctggtc gaacgaagca caagagcgtt acgtgctggc gattgcgccg gcggatctgc
     1861 cggccttcga agcgatgtgc gagcgcgagc gctgcccgtt cgcggtggtc ggcaaggcca
     1921 cggccgagcg tcaactgaag gtgctcgact ccgaatcgaa ccacgacgtc gcacacgagc
     1981 cggtcgacat gccgatggaa gtgctgctcg gcaaggcgcc gcgcatgcat cgcgacgtga
     2041 agcgcgtcga gcagaagctc gagcctgtgg atgtcaccgg catcgccttg tcggaagtcg
     2101 cggtgagcgt gctgcgtcac ccgacggttg ccagcaagtc gttcctgatc acgatcggcg
     2161 accgctcggt gggcggcact accgcacgcg accagatggt cggcccgtgg caagtgccgg
     2221 ttgccgacgt cgccatcacc acgatggact acgcgggctt ccgcggcgaa gcaatgacaa
     2281 tggccgagcg cacgcctttg gcggtcatca acgcgccggc ctcgggccgc atggcggtcg
     2341 gcgaggcggt caccaacatc gcggcggcgc cgatcgcgtc gctggataaa ttgaagctgt
     2401 cggcgaactg gatggccgcg tgcggcgcag cgggcgaaga cgcggcgctg tacgacacgg
     2461 tcaaggcgat cggcatggaa ctgtgcccgg cgctcggcat cagcattccg gtcggcaagg
     2521 attcgctgtc catgcgcacc aagtgggaag accgtggcgt ggcgaaagaa gtggtcgcgc
     2581 cggtctcgtt gatcatctcg gcattcgcgc cggttgaaga cgtgcgccgc cacctcacgc
     2641 cgcaactgcg ccgcgcgagc ggaacggacg gcgtgggcga ctccgtgctg atcgcaatcg
     2701 acctcggccg cggcaagcag cgcctcggcg gcagtatcct cgcgcaagtc acgcagcagg
     2761 tcggcgacac ggtgccggac gtcgacgacg cgcaagacct gaagcgcttc ttcacggcga
     2821 tccaggcgct gaacagcgac ggcaagctgc tcgcgtacca cgaccgttct gacggcggcc
     2881 tgtgggcgac ggtgtgcgaa atggcgttcg cgggtcacgt cggcgtgtcg ctgaatgtcg
     2941 acatgctggt gctcgatccg aaccacgaat ccgattacgg cgatgcgaaa gactgggcga
     3001 agcagaccag cggccgccgc gaggaccgta cgatccgcgc gctcttcaac gaggaactgg
     3061 gcgccgtgat tcaggtgcgc gcggcggacc gtgacgcggt gctgggcgcg ttgcgcgaac
     3121 agggcctgtc ggcgtgctcg cacgtgatcg gcaagaccaa cgagcgcgac acgatcgaaa
     3181 tctatcgcga cgcgaagaaa atctacgaag cgccgcgcac ggaactgcat cgcacgtgga
     3241 gcgaagtgag ctggcgcatt tcgcgtctgc gcgacaaccc ggcttgcgcc gatgccgaat
     3301 acgacgcgct ctccgacgcg gccgatccgg gcatctcgcc ggtcctgacc ttcgatccga
     3361 cggaagacgt cgccgcgccg ttcatcgccc aaggggactt ccttcggggc gtcggcaagg
     3421 gtgcacgccc gcgtgtcgcg atcctgcgtg agcagggcgt gaactcgcac ctcgaaacgg
     3481 cctacgcatt cgaccgcgcc ggtttcgacg cgcacgacgt gcacatgagc gacctgctgg
     3541 ccggtcgcgc caacctggcg gatttcgccg gtgcggtggc gtgcggcggc ttctcgtacg
     3601 gcgacacgct gggtgccggt gaaggctggg cgaaggcgat ccgcttcaac tcgcaactcg
     3661 ccgacatgtt cgcggcgttc ttcggccgcg aagatacatt cgcactcggt atttgcaacg
     3721 gttgccagat gatgagcagt ctcgcctcga tgatcccggg cgcggaagca tggccgaagt
     3781 ttacgcgcaa caaatcggaa aaattcgaag cgcgcttctc gctggtcgaa gtgcaggcgt
     3841 cgccgtcgat cttcttcgcc ggcatggaag gttcgcgcat tccggtagcg attgctcacg
     3901 gcgaaggtta cgcggacttc tcgcagcaag gcgatgcgtc caaggtggcc gtggcgatgc
     3961 gcttcgtcga ccaccgtggt caggcaacgg agcaatatcc gttcaatccg aatggatcgc
     4021 cgaacggcat cacgtcggtc acgacacccg atggccgctt cagcgtgctg atgccgcata
     4081 ccgagcgcgt gcatcgtgcg gtgcagatga gctggcatcc ggaaggttgg ggcgagggca
     4141 gcacggacgc gagcccgtgg ctgcgcgtgt tccagaacgc acggcgctgg ctcggttgat
     4201 gtaggcgggc agtctgctgc gctatctacg cgcggcagac tgatgcgggg tgcgtatccg
     4261 ctcgggtacg aaagagccta actaacccaa tcctcccttc ttcgtatccg ctcgggtacg
     4321 aaagcccgat atgtgcaccc agaattcatc attcgtaccc gctcgggtac gaattcacgt
     4381 cgccgccttc gtctgatggc ataatgttcc cgagcgggta cgaacgcgag gggcgaagaa
     4441 tgcggcaatc cgacgttttc attagcaaca tgacggtgtt aggtgatctt ttgcgggcgt
     4501 cacggcttgc acaagggctg acgcgcgacg acgtcgccat cgccacgggt ctgtcgccca
     4561 agttcatcac acatatcgag gcgggcaagc cgaccgctca gatcggcaag gtgctccacc
     4621 tgctcggcga actgggcatt gcccttcgcg cacaggtttc cgtggacatt ccgcctgcct
     4681 tcgccaccca accgcgccat cgccgccggg ttactcatgg cagctagaac gctcgtcgca
     4741 tatgcgaatg gccagcgcgt tggcgcggtg agcgatgagg gcggtatctg gtcatttacc
     4801 tacgataaag cctggctcga ccgcgaagac gcgttcgtgc tttcacccgc tttccctctg
     4861 catgcagaac cttttgtcga tggctcgagc cagcggccgg tgcaatggtt cttcgacaat
     4921 cttttgcctg aagaggagat gcgcacggcg ctcgctcgcg aagccaaagt caacgcgagc
     4981 gatgcatggg ggctgcttgc atactacggt cgcgagtcgg ccggcgcctt gacgctcctc
     5041 gccgagggcg aacaggaagc ttccggtggt ctgcagcctt tgtcgtacgc ggacctggag
     5101 gctcgcattc aggccatgcc gcaacgcgcg ctcatcgcca ctgcgcccaa gcgcatgtcc
     5161 gcagcgggcg cccaacaaaa actgctgctg tccttgcaag gcgaggcgcc gaacgaccgg
     5221 ctctttgagc ccgtgggtca tgagccgtca atgcatctgc tcaagcctga catgcgaggc
     5281 accggctacc ctcattcagc gataaatgaa tttttctgca tgcagctggc acgagcgatg
     5341 ggcgtgcgcg tgccgcctac gcattttatg cgcgtgcctt cggcctgcta tgtgatcgat
     5401 cgtttcgacc gcgatacggc ggccgagccg gttcggcgct tacatacggt cgacgcaatg
     5461 caactgtcga actacgaccg caacttcaaa taccagaacg ccacggcgca ggtactcagg
     5521 gatgcgattg aaaagacagg cacgcgggcc atggcacgtc tcgatgtatt ccgctgggcg
     5581 gtcttcaacg tgctcattgg caacgcggat tcccacctga aaaacctctc gttcttcgtt
     5641 aacgctcgag gctataggtt ggcgccgttt tacgatctgg tcagcacagt cgtctaccat
     5701 acgccgacct atcagccgca acagcgggac cgttggccgc agtgcgacct gaccatgccg
     5761 atcggagcgg ccacccagtt cgcggacgtt tccagacaga acatgctggc attcggcgaa
     5821 gcgcttggcc tcgcgccaaa aggctcggaa aaattgctgg atgagatgct ggccgttgtc
     5881 ggcacccggg tagcggacac ccggcgagcc gtcggcgagg tcgcaaagcc gaatgccggc
     5941 gaagtccggt tgctcgactc gatcgttgcc atgccgcttg cagagatgag cagggcactg
     6001 cgcaagtgat aacggcattc ttgctgagtt agttgattcc acataaacga ggccttgttc
     6061 agtcgatggc tgaacagggc cttgttttat gcatcgaaaa cggccagcgg gagctgaaac
     6121 gctgaacaag acggagcata aaaaaaccgc cacgagggcg gtcttttctg aagcagcagg
     6181 ccaacaacgc ttactgaatc ttcgcgttct tacgcagacc ttcttcgaac gcctgcagct
     6241 tttcctgctg gatctgctgc acgatctgcg gacgcacttg ctcgagcggc ggcggcgtga
     6301 cgttgcgcac gtcgtcgaca cggatgatgt gccagccgaa ttgcgtatgc accggcgtat
     6361 cggtcatctg gcctttctgc agatgcgtgg ccgcgtccgc gaattcaggt acataggcct
     6421 tcgggtccga ccagtccagg tcgcc
//
LOCUS       DATASH010000233        12816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3986058,
            whole genome shotgun sequence.
ACCESSION   DATASH010000233 DATASH010000000
VERSION     DATASH010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 12816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12816
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3986058"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /locus_tag="VHV99_06525"
     CDS             complement(<1..918)
                     /locus_tag="VHV99_06525"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3635132.1"
                     /translation="MSSKVVSTGNMASGREQIHMGFVIAWLFCAAFYFMQYMLRSAPG
                     VMIPELSAAFARDALGVGALVGLYYYTYALFSIVCGALLDRLGGKAVIPAGIVLVAVG
                     AALFGLGSVEAAQFGRLLQGAGSACGFIGAVYLATHGFPPRSLATAVGFTQCFGMLGG
                     SAGQFAVAPLIHGLITWQQFWWLSGLVIFLIAALVFVVTPAGHDDSPASQGSWLKMFT
                     PYKEVLSNPQSYLCGFCAGLLFLPTTIGDMIWGIPFLRNGLEASYTEAVNRASMVPLG
                     WVIGCPLLGYLSDRLGRRKPVLMAGALLML"
     gene            complement(985..2706)
                     /gene="poxB"
                     /locus_tag="VHV99_06530"
     CDS             complement(985..2706)
                     /gene="poxB"
                     /locus_tag="VHV99_06530"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HEX3635133.1"
                     /translation="MATIAADYMVGALINAGVKRVFGVVGDSLNGFTDSLRRDGSIEW
                     IHMRHEESAAFAAGAEAHLTGQLAVCCGSCGPGNLHLINGLFDCHRSGVPVLAIAAHV
                     PSTEIGIDYFQATHPEVLFKECSHYVELVHSPDQLPQILGRAIRTAVARKGVAVVVIP
                     GNVALGPLTKPVPRWSAPAAPVIRPADDQVAELARLLNGSSRVTLLCGAGCKDSHAEV
                     VELARKLQAPIVHSLRGKEYIEYDNPFDVGMTGLVGFSSGYSAMKSCDMLLMLGTDFP
                     YRQFYPEKVTIAQVDTRAEALGNRCPIQLGVLGTVKDTLGELLPLIRENTDSSFLNRA
                     LDDYRKARESLNALAEPEVGSSTIHPQYVTRLVSELADEDAIFSCDVGTPIAWTARYL
                     KMNGKRRLVGSFNHGSMANSLLHSIGAQATFRDRQVISMSGDGGFTMMMGEFLTLIQT
                     GLPVKVVVLNNGTLGFVEMEMKASGFVDTGCDLKNPNFAAMANAIGIKGIRVEHPAEL
                     EGALGEAFAHPGPALVDVVSARQELVMPPSTTADQAYHFGLFMLRAVMDGRGQQLIDL
                     AKVNLFR"
     gene            complement(2777..3130)
                     /locus_tag="VHV99_06535"
     CDS             complement(2777..3130)
                     /locus_tag="VHV99_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3331 domain-containing protein"
                     /protein_id="HEX3635134.1"
                     /translation="MMALLSSEIEIQLPSVPARVRPKRGECDFSKCAIRVLERHGRTS
                     ATVAWSDATSCCYGEQVWRRRTARKAGVCALSGQGIATGDAIYRPRPARPAPRNIDAM
                     ILATVMEAIPVEVVA"
     gene            3643..4284
                     /locus_tag="VHV99_06540"
     CDS             3643..4284
                     /locus_tag="VHV99_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3635135.1"
                     /translation="MESASEIVYVVDDDRRVREALAVLLRANGRNVHAFNSGRAFLDT
                     LRQDLVSCLILDLRMPGMSGLEVQKLVSTDSRIPVIFITGMGDVPSTVLAMKGGAVDF
                     LTKPVDEDALLRAVDAALARAHVLRKAAQDTAALQALYESLTPREQELLPLLASGLLN
                     KQAAAVLGITEYTVQVHRGHIMRKMQAASFATLVRQASKLQLETRPTATFPEG"
     gene            complement(4259..5551)
                     /locus_tag="VHV99_06545"
     CDS             complement(4259..5551)
                     /locus_tag="VHV99_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3635136.1"
                     /translation="MLAPAAKRDVRESVALSAKVWFETLLSGQPPSAQDMEIFREISQ
                     RREHQRVPLQSLLQALRLGLREIWSTHIALGVLDERIAKELLADVSLYLLDHFDLIAQ
                     IIVRAYLTDHHRQPRWAGLAIAIEEGARRSESVRHHLVTLLEQLSEEPDLLSRLELDH
                     AREQRGTEAAGTQVMSFFGLDVDIDDGAELSEALRDAQERLAQASRLAAVAELSASIA
                     HELNQPLQAIVAHGRACVRWLSATPPRIDEAKYSAEQVLRDANSAAEVVSRIRALFRH
                     TAPQKVELDINKLILQVCTLMADDIQRNAVSLETTLGEDVSKISADAVQIQQVIVNLV
                     RNANDALAATEERPRRLVIRSHRDADNVVVDVEDHGTGQVDFEKIFEPFFTTKETGMG
                     MGLAICRSIIEAHAGRIWAVRNVSRGVTFSFSLPGRLP"
     gene            6024..8795
                     /locus_tag="VHV99_06550"
     CDS             6024..8795
                     /locus_tag="VHV99_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX3635137.1"
                     /translation="MRVVGEAPASGPHIFLATKVVLPRLPADLISRPRLVGLAERAAS
                     KRLTVIKAPAGFGKTSLALIWLERLRTNGARVAWLSLDAEDDEPAHFLNSLARALRHA
                     CGDVGAAAISLTADAAFVPPRAVVTTLINELLEVDDEVVLFLDDYHLISLPAVHQTMS
                     FFIEKAPSHVHVVLGSRTDPSLPLARLRAGGDLLEVDASALRFDFDETKRLVEHALPG
                     VLSASHVEMLYANTEGWAAALRISMSVLSRTQDQPAEKDMVPSGTSRPFAAYIEHMLE
                     RLPADMVEFMLRTSILDRLSAPLCEAITGQRTSQRMLDEIAEGRLLLQALDLDGQWFR
                     YHHLMSEYLRAKLEAQHPDEVAELHCRACEWYGAQALWTEAVKHAIAAGSTDNAIRLM
                     EQCAMALVKKGDLLTLLGLQRRFPAGLAGAQVKVTLAIAWGMALAMHFDEALTMLDAL
                     EHDMPSHTGKEAEDIRWECQAIRSVVAGLKDHPERALAIAEPCLARPSADIWTTNVAS
                     NVVRFGHWKAGKLEALYATPWMSYSSEEHQRNLYSSVYRLCLLGHAEMQQLHFGLAER
                     YFTESMRLAERHAGPDSISVALCAPMIGQIQYEQGRLDDAEKRVAGLMPVIDVAVLLD
                     SVLIAYRLLIRIAAARSDMRQAYALLDRAQALGCARQWERLTAATIAERIRLYVAEDR
                     LMEAAACLTQLDQLARSGSHPSRSIALEIENYRALAAASVAAAQHRTEEAAAVLSAAL
                     QSAEPRHGDCLAIRLRTMLALVWLAADERARAIEVFRDVLKVAAPAGVYQSILDVGRD
                     VGALLRVVGEDARHAAHAAPTTPAGQANELISYIDHLLDGWRILYQPGGNRQRDTERG
                     SLSAREREIVDLIARGLSNKEIANTLGIGAETVKSHVKSIFMKLGVDRRTQAVARVRD
                     LGLVGPAMD"
     gene            9221..9628
                     /locus_tag="VHV99_06555"
     CDS             9221..9628
                     /locus_tag="VHV99_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012405641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEX3635138.1"
                     /translation="MDNAAKEAVGARIQPHQAMTEFARVLWHLRGILAVLLVLFLVLS
                     AAMYYVGGAVDAATRTPSSPGQTFYFCAVTALTIGYGDVIPTTTPGRIVAVALGLLGV
                     LITGVVTATAVYAIQVAAHRAGLDLASRGRGQD"
     gene            9908..10546
                     /locus_tag="VHV99_06560"
     CDS             9908..10546
                     /locus_tag="VHV99_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3635139.1"
                     /translation="MESASELVYVVDDDRRVREALSVLLRANGRDVHVFNSGREFLDT
                     PRQDGVSCLVLDLRMPGLSGLEVQGLVSTDSHIPVIFISGMADVRSTVLAMKGGAVDF
                     LTKPLDEDALMRAIDSALARAHVRRKAAEDTAALQALYESLTPREQELLPLLAGGLLN
                     KQAAAVLGITEYTVQVHRGHIMRKMQADSFAALVILASKLQLETSSAATVGA"
     gene            10592..10957
                     /locus_tag="VHV99_06565"
     CDS             10592..10957
                     /locus_tag="VHV99_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3635140.1"
                     /translation="MSTPPVRTVAVIDDDKRVLTSLANLLASGGYETRAYESALDFFS
                     DGYSGLICVITDLGMRPIDGIQVLDRTVHSDAATPVIIITGKPGEHTESYYLQRGALG
                     FFRKPVDGDALLDLLDSIP"
     gene            11444..11737
                     /locus_tag="VHV99_06570"
     CDS             11444..11737
                     /locus_tag="VHV99_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HEX3635141.1"
                     /translation="MPLAGGRGSKRAIATATIAAIAAIPMSGCTGSPSIGVLGAYFPD
                     WLFCIVAGVVLTVVIYLILKRLQADHLLGPAAVVYPTLVTLLALAAWLTLFQH"
     gene            11757..12815
                     /gene="mdtN"
                     /locus_tag="VHV99_06575"
     CDS             11757..12815
                     /gene="mdtN"
                     /locus_tag="VHV99_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtN"
                     /protein_id="HEX3635142.1"
                     /translation="MATTASSTSRRKWPAIVLVVVTLLLLLFVIRLLDRAPRTDDAYV
                     YADTIDVVPEVNGRIVELPVRDNQAVKQGDLLFRIDPRPYQDALTRGNASLVALDRQI
                     ELTQRTVNAQQYNAQSVRAAVERARAAASQAADTLHRMEPLLSKGYVSAEDVDRARTA
                     QRATQAELVAAQLQAQQAAAAVSGVDALVAQRAVVMAEIASAELNLEYATVRAPFDGR
                     IVSLKTATGQFASVLKPVFTLIDTRHWYVVANFRETELKGIGVGTPATVYLMSDTGQR
                     FRGSVDSISYGVAPSEAGLALPGGLPNIQRTLNWVHVSQRFPVKIRVDNPNPALFRLG
                     TSAVAVLEPGRGNDSERH"
BASE COUNT         2452 a         3866 c         3974 g         2524 t
ORIGIN      
        1 aagcatcaac agcgcgccgg ccatcaacac cggtttgcgc cggcccagac ggtcggacag
       61 gtagcccaac agcgggcagc cgatcaccca gccgagcggc accatgctcg cgcgattgac
      121 agcctccgta tagctggctt cgagaccgtt gcgcaggaag ggaatgcccc agatcatgtc
      181 gcctatcgta gtgggcagga acagcagacc ggcgcagaag ccgcaaagat acgactgcgg
      241 attggacagt acttccttgt aaggcgtgaa catcttcagc caggatccct ggctggccgg
      301 actgtcgtca tggcctgccg gcgtcacgac gaacaccagc gcggcgatca gaaagatgac
      361 gaggccggac agccaccaga actgctgcca tgtgatgagg ccatgaatga gcggcgccac
      421 ggcgaactga cccgcggagc cgcccagcat gccgaagcat tgcgtgaatc ccactgccgt
      481 ggcgagcgaa cgcgggggaa agccgtgcgt ggcgagatac accgcgccga tgaacccgca
      541 cgccgaaccc gcgccttgca gcaggcggcc gaactgcgcg gcctccacgc tgcccaggcc
      601 gaacagcgcc gcgccgacgg caaccagaac gatgccggcc ggaatgacgg ccttgccgcc
      661 gagccgatcg agcaacgccc cgcaaacgat cgagaacagc gcgtaggtgt agtagtacag
      721 gccgacgagc gcgcccacgc ccagcgcgtc gcgcgcgaac gccgcactca gctccgggat
      781 catcacgccc ggcgcggaac gcagcatgta ctgcatgaag tagaacgcgg cacaaaagag
      841 ccaggcgatg acgaagccca tgtgtatttg ctcgcggcct gatgccatat ttcctgtgct
      901 gacgaccttg ctggacatgg ttagcgttcc attgggttgc tgttcgaacg ggtacgggcc
      961 agcgcgcggc gcggacgcac tgcgctagcg gaacagattg acctttgcga ggtcgatgag
     1021 ctgctggcct cggccgtcca tgacggcacg gagcatgaac aaaccgaagt ggtaagcctg
     1081 gtcggcggtc gtcgaaggcg gcatcaccag ttcctgacgt gcgcttacca cgtcgacgag
     1141 cgccggaccc ggatgggcga atgcttcacc gagcgcgcct tccagctccg cggggtgctc
     1201 gacgcggatc cctttgattc cgatcgcgtt ggccatggcg gcgaagttcg gatttttcag
     1261 gtcgcatccc gtatcgacga acccgctggc cttcatctcc atttccacga agcccagcgt
     1321 tccgttgttg agcacgacga ctttgaccgg caaacccgtt tggatcagcg tcaggaactc
     1381 gcccatcatc atggtgaagc cgccgtcacc cgacatcgaa atgacctgcc ggtccctgaa
     1441 cgtggcctgc gcgccgatcg agtgcaacag cgagttcgcc atcgagccgt gattgaatga
     1501 gcccaccagg cgtcgtttgc cgttcatttt cagataccgg gctgtccatg cgatgggggt
     1561 gccgacgtcg caggagaaga tcgcgtcttc gtccgcgagt tcgctgacga gccgggtcac
     1621 gtactgcgga tgaatggtgc tgctgcccac ttccggttcc gccagcgcat tcagactctc
     1681 gcgagccttg cgatagtcgt cgagcgcgcg attcaggaaa ctgctgtccg tgttctcgcg
     1741 gatcagtgga agcagttcgc cgagcgtgtc cttgactgtt cccaatacgc cgagttgtat
     1801 cgggcaccgg ttgccgagcg cttccgcccg tgtatcgact tgcgcgatgg tcaccttctc
     1861 cgggtagaac tgtcgatagg ggaaatcggt gccgagcatc agcagcatgt cgcaactctt
     1921 catcgcggag tagccggacg agaagccgac aagtcccgtc atcccgacat cgaaagggtt
     1981 gtcgtattcg atgtattcct tgcctcgcag cgagtgcacg atgggcgctt gcagcttgcg
     2041 ggcgagttcc acgacttccg catgggaatc cttgcaaccg gcgccacaca gcaacgtcac
     2101 gcgcgacgat ccgttcagga gacgcgcgag ttccgcgacc tggtcgtccg ctggacgaat
     2161 caccggagca gcgggcgcgc tccagcgcgg caccggtttt gtgagcggcc ccagtgccac
     2221 atttccaggt atgacgacca cagcgacgcc tttacgcgca acggccgtcc ggatggcacg
     2281 gcccaaaatc tgcggcagct ggtcaggact gtgaaccagt tcgacgtagt ggctacattc
     2341 cttgaacagc acctcggggt gggtcgcctg aaaatagtcg atgccgattt ccgtgcttgg
     2401 gacatgtgcg gcgatagcga gtaccggcac accgctgcga tggcagtcga acaagccgtt
     2461 gatgaggtgc aggttgcccg gaccgcaact cccgcagcag accgcgagtt gtcccgtgag
     2521 atgagcctcg gcaccggcgg cgaaagccgc gctttcctcg tgccgcatat gaatccattc
     2581 aatcgaacca tcccggcgca gggagtccgt gaagccattc agcgaatctc ccaccacacc
     2641 gaacacacgc ttcacgccgg catttatcag tgcaccaacc atgtagtcgg cagctatcgt
     2701 tgccatttcg catctccata aattaatgaa ttgcttcaaa tcggatgtgt ccctgtgaag
     2761 aggggcacct gagcgctcac gcaacgacct ccacagggat agcctccatg actgtggcca
     2821 gaatcatcgc gtcgatattt cgcggtgccg gccgggcagg ccgtggtcga tagatggcgt
     2881 cgcctgttgc gatgccctgg ccgctcaggg cgcacacccc ggccttcctt gccgtgcggc
     2941 gccgccatac ctgctcgccg tagcagcagg aggtcgcgtc gctccacgcg actgttgccg
     3001 aggttcgtcc atggcgctcc agaacccgga tggcgcattt cgaaaaatcg cattcgcccc
     3061 gcttggggcg cacgcgggca ggcacactgg gcaactgtat ttcgatctcg ctcgagagga
     3121 gcgccatcat ggactgccac gttctgtctg catcaaaggg agctggcatg atggggttcc
     3181 tcgattctcg ttagacaaat aggcgttccc gcgattgacg tgaagcgcaa cagaaggctg
     3241 catgacattg tcgaatgacg cgtcgtgcgt gttcctgtcg ggtatcggcg tcgaattgcc
     3301 gggaagacga cgcgcgcgat caatgtcggg tgatgcgacg ccagcggatt gcccatggtc
     3361 ttatgcggcg tgccggggtt gagccacgct tggccttgac tggatgatag gcagcaggcc
     3421 cgcggaccgc tattacagga atgtggagtg catccgccat gcgacgatgc cattacatat
     3481 tcatgtaatg cagaacacga ctccccgtgc ttccagaaaa cgattcggat agctataatt
     3541 tctcgtcgta aacgccccgc ttgagaggcg catttagcgg gtctatcgga gaccggctgg
     3601 accgacgtta ggagaagttt tgcctaacca cggaggggtt ccatggaatc cgcaagcgaa
     3661 atagtctatg tagtcgacga cgacagacgg gtgcgagaag cactggctgt tctactgcgt
     3721 gcaaacggaa gaaacgtcca cgcgttcaac tccggcagag cgtttctgga cacattgcgt
     3781 caagacctcg tttcatgcct gatcctcgat ttgagaatgc ccggcatgag tggcctggaa
     3841 gtccagaagc tcgtcagcac ggactcacga attcctgtca ttttcattac cggcatgggc
     3901 gatgtgccgt ccacggttct tgccatgaag ggcggggcgg tcgacttcct caccaagccc
     3961 gtcgacgaag atgcgttgtt gcgcgcggtc gatgcagccc tcgcgagagc ccacgttctt
     4021 cgcaaggcgg cgcaagatac ggcggcgctt caggcgctct acgaatcgct gacgccgcgt
     4081 gagcaggagc ttttgccgtt actcgccagc ggcctgctca acaagcaggc ggcggcagta
     4141 ctgggtatca ccgaatacac cgtgcaggtc catcgcggcc acataatgag aaaaatgcag
     4201 gcggcttctt tcgctacgct ggtcaggcag gcaagcaagc tacaactcga aacccggcct
     4261 acggcaacct tcccggaagg ctaaaactga aggtgacccc tcgcgacaca ttgcgcacgg
     4321 cccaaatccg acccgcgtgt gcttcgatga tggaccggca gattgccagt cccatgccca
     4381 tccctgtctc cttggtagtg aagaacggct cgaagatctt ctcgaagtcg acctgtcccg
     4441 ttccgtgatc ttccacgtcg actactacat tgtccgcatc gcggtgcgaa cggatcacta
     4501 gccgcctcgg tcgttcttct gttgccgcta atgcgtcgtt tgcattgcgc acgaggttca
     4561 cgatcacctg ctgaatctga accgcgtccg cgctgatctt cgacacgtct tcgcccaggg
     4621 tcgtctcgag cgaaaccgcg ttgcgctgga tgtcgtcggc catcagcgtg catacctgaa
     4681 gaatcagttt attgatgtcg agctcgacct tttgcggagc cgtgtgcctg aagagcgcgc
     4741 gaatccggct taccacctct gccgcggaat tcgcatcgcg cagaacctgc tccgccgagt
     4801 atttggcttc gtcgatcctg ggcggcgttg cggagagcca tcgcacgcat gcgcgcccgt
     4861 gcgcgacaat ggcctggagt ggctggttga gttcgtgggc gatggaggcg gacagttctg
     4921 caacggccgc gagcctcgat gcctgcgcca gcctctcctg tgcatcgcgc aaggcctccg
     4981 acagttccgc gccgtcatcg atgtcgacgt cgaggccgaa gaaactcatc acttgcgtgc
     5041 cggccgcttc cgtaccgcgt tgctctcgag cgtgatcgag ttcgagcctt gacagcaggt
     5101 ccggctcttc cgataactgt tcgaggagcg tgacgagatg gtgccgcacg ctttcggagc
     5161 ggcgcgcgcc ttcctcgatg gcgatcgcca atcccgccca tcgcggttgc cgatggtggt
     5221 cagtcagata ggcccggaca atgatctgcg caatcagatc gaagtggtcg aggagataca
     5281 gcgagacgtc ggccagaagc tctttggcga tacgctcgtc cagcacgccc agcgcgatgt
     5341 gggtgctcca gatttctcgc aaccccagac gcaacgcctg aagaaggctc tgtagcggca
     5401 cgcgttgatg ctcgcgtctc tggctgattt cccgaaagat ttccatgtcc tgtgccgatg
     5461 ggggttggcc cgacaacagt gtctcgaacc atactttggc cgacagcgcg acggattcgc
     5521 gaacgtcccg tttggcggcc ggggcgagca acctgtatcc gtcgagctga gtcagtatcg
     5581 caagcgtgcg ttcgacgact agcgtcagat ctgctgcgat ggactcggtt ttttcacgca
     5641 ggtgttggct gattcgtgga actttggcta gcacggatat ctgtctatca ctaacgggcg
     5701 cacgatttcg actgacaagg agcgccggcg tagcgacttt aagggctacg gaccggttgc
     5761 cgcctgatac gccgcaatgc gctgcgaggc ggcgtatcat ggggcgaaaa accagtcgtt
     5821 ctcagtgagg cgccagtgaa tcgcccggca gtgcccggca tgcacacggc cccttgtctg
     5881 gtgcaggcat gatctcctgg cgcggcgcga cctgccatcc cccttacagg ggagaggctc
     5941 accctccccc aagcagggga tgcgcttcaa cacaggggat ggcggcaggc gcggccaccc
     6001 caatgacact gtgtgactga caaatgcgtg tggttggaga agcgcccgcg agcgggccgc
     6061 acattttcct tgcaacgaag gtcgttctgc cgcgcctgcc ggcggatttg atcagtcgtc
     6121 cgcggctcgt cggtctggct gagcgcgccg cgagcaaacg tcttaccgtg atcaaagcgc
     6181 ccgccggatt cggcaagacg tcgttggcgc tcatctggct cgagcggctg cgcacgaacg
     6241 gtgcacgggt ggcgtggctg tcgctcgacg cggaagacga cgaacccgcg cacttcctca
     6301 atagtctggc gcgagcgcta cggcatgcct gcggcgatgt cggcgccgcg gcgatcagcc
     6361 tgactgccga cgccgccttc gtcccgccac gcgccgtcgt taccacgctg atcaacgaac
     6421 ttctcgaagt cgacgacgaa gtcgtgctgt ttctggacga ctatcacctg atcagccttc
     6481 cagcagtaca ccagacgatg tcgttcttta tcgaaaaggc accgtcacat gtgcatgtgg
     6541 tgctcggcag ccgcaccgat ccatcgctgc cgctcgcgag gttgagggca ggaggcgacc
     6601 tgctggaagt cgatgcgtcg gccttacgtt ttgacttcga cgaaacgaag cgcttggtcg
     6661 aacacgcatt gcccggcgtg ctgagcgctt cccacgtcga gatgctttat gcgaacacgg
     6721 aaggctgggc tgctgcgttg cgcatttcga tgtccgtgct gtcgcgcacg caagatcagc
     6781 cggccgagaa agacatggtc ccgtcaggta cgtcgcggcc attcgccgcc tatatcgaac
     6841 acatgctcga gcgattgccc gccgacatgg tggagttcat gctgcgcacg tcgatactcg
     6901 atcgcttgag cgcgccgctc tgcgaggcga taacggggca gcgcacgagc cagcgcatgc
     6961 tggacgaaat cgccgagggc cgcttgctgt tgcaagcgct cgacctggac ggccaatggt
     7021 tccgttatca ccatctgatg agtgagtacc tgcgcgcgaa gctcgaggcg cagcatccgg
     7081 acgaggtggc ggagctgcat tgccgcgcgt gcgaatggta tggcgcgcag gcgctgtgga
     7141 ccgaggccgt caaacatgcg atcgccgcgg gcagcacaga taacgcgatt cgtctcatgg
     7201 aacagtgcgc gatggcgctg gtcaagaaag gcgatctgct cacgctgctc gggttgcagc
     7261 gccggtttcc ggcaggtctg gccggtgcac aggtgaaggt cacgctggcg atcgcctggg
     7321 gcatggcgct cgccatgcat ttcgacgagg cactcacgat gctggatgcg ctcgagcatg
     7381 acatgccgtc ccacacgggt aaggaagccg aggatatccg ctgggaatgc caggcgatcc
     7441 gctcggtcgt cgcgggactg aaggaccatc ccgaacgggc gctcgcgatc gctgagccgt
     7501 gcctcgcgcg gccgtccgcc gatatctgga ctaccaatgt ggcatccaat gtcgtgcgct
     7561 tcgggcattg gaaggccggc aagctcgaag cgctttatgc aacgccgtgg atgtcctatt
     7621 caagcgaaga acaccagcgc aacctgtact cgtcggtcta ccgcctgtgc ctgctggggc
     7681 atgcggagat gcaacagttg cactttgggc ttgccgagcg gtatttcacg gaatcgatgc
     7741 gactggccga acgccatgcc ggtcccgatt cgatttcggt cgcactgtgc gcgccgatga
     7801 tcgggcaaat ccagtacgaa caaggccgac tggacgacgc cgaaaagcgg gttgccggtt
     7861 tgatgccggt gatcgacgtt gcggtcctgc tcgatagcgt gctgattgcc tatcgcctcc
     7921 tcatccggat cgcagccgcg cgctcggata tgaggcaggc gtacgcgctg ctcgatcggg
     7981 cccaggcgtt gggttgcgca cggcagtggg aacggctgac cgccgccacc attgccgagc
     8041 gcattcggct ctatgttgcg gaagacaggt tgatggaggc ggctgcatgt ctgacgcaac
     8101 tcgatcagct agcgcgttct ggttctcatc cttcacgctc catcgcgctc gagatcgaga
     8161 actatcgtgc actcgcggcg gcctctgttg ccgcggccca gcaccgcacc gaagaggccg
     8221 ccgcggtgct gagcgccgcg ctgcaaagcg ccgaaccacg gcatggcgat tgcctggcga
     8281 tacgtttgcg caccatgctt gcactggtct ggctggcagc ggacgaacgc gcccgtgcga
     8341 tcgaggtctt tcgcgacgtc ctgaaggtcg ccgcgcccgc gggtgtgtat caatccattc
     8401 tcgatgtggg tcgggacgtc ggagcgttgc tgcgggttgt tggcgaagac gcgcggcatg
     8461 cggcacacgc ggcacccacg acacccgcgg ggcaagcgaa cgagttgatc tcctacatcg
     8521 atcatctgct cgatggctgg cgaatacttt atcaaccggg cggcaaccgt cagcgtgaca
     8581 ctgagcgcgg ctcactgagc gcaagggaac gcgaaatcgt cgacctgatt gcacgaggac
     8641 tgtcgaacaa ggaaattgcg aatacgcttg gaattggcgc tgaaaccgtc aaatcccatg
     8701 tgaagagcat cttcatgaaa ctaggcgtcg atagacgcac tcaagccgtt gcacgggtac
     8761 gggatctcgg tcttgtgggt ccggcaatgg attagcgtta gcgtccagat agctaacgtc
     8821 gcacagacgg cccacgataa tctcgctgaa tgtccctcat acgcggtcgg tgccggcaaa
     8881 aatcagagcc gccggaatcg tcgggcggaa gcgcgggcgg ccatgctgtg cactacatga
     8941 atatgtaatg gcacccgcca gggcgaagtt gtaactttag cttttcgcct cggaataaat
     9001 cctttgacgc tgaccgggct tgttcccacc agttgccatt tctgtaccca gcagacgctg
     9061 gatcaggact cggtgcaact cagcaatcgc tcatgttcga caatcactcc cgctctccgg
     9121 cgccttcctg gctgggagaa gcattcagga tcgccccggt agtggtatcg cattcaggct
     9181 ccggaaacag agcgtctgga acatctgtga ggtccatgac atggataatg ccgccaaaga
     9241 ggcagtcggc gcccgaatcc agccgcacca ggcaatgacc gagtttgcca gggtcctctg
     9301 gcacctgcgc ggcattctgg cggtgctgct ggtacttttc ctggttttgt cggctgccat
     9361 gtattacgtc ggaggggctg tcgatgcagc cacgcggaca ccatcatcgc caggtcaaac
     9421 tttctacttt tgcgcggtca cggcgctgac catcggttat ggcgacgtga ttccgaccac
     9481 cacgcccggc cggatcgtcg cggttgcgct tggactactc ggcgtgttga taacgggtgt
     9541 ggtaacggcc accgctgttt atgccatcca ggtggctgcg catcgggctg ggctagacct
     9601 ggccagtcgc ggtagagggc aagattgagg acgttctcct ttcctcgtgc tccgttgtgc
     9661 cttgctggac tgattgcaga atcatgtaat agccgtcctt cgccccgccc actagcatca
     9721 ggaaccgggc acgttgagcc atttagctca gtgaccatgg cctggcgatt gcacgacgga
     9781 gctcgacatg accgcgggcc tgacgagcgt ccggccgggc cattcacggc gatcggtttt
     9841 cagcaatggc gtggctgacg gtacgggaag ccctgcatct gaccagacct aactacaaag
     9901 gggttccatg gaatccgcaa gtgaactcgt gtatgtagtc gacgacgaca ggcgggtgcg
     9961 agaagcactt tctgttctac tgcgcgcaaa cggaagagac gtccacgtat tcaactccgg
    10021 cagagagttt ctggacacgc cgcgtcaaga tggcgtgtca tgcctggtcc tggatttgag
    10081 aatgccaggc ctgagtgggc tggaagtcca ggggctcgtc agcacggact ctcacattcc
    10141 cgtcattttc atttccggaa tggcagatgt gcgatccacg gttcttgcca tgaagggcgg
    10201 ggcggttgac ttcctcacca agcccctcga tgaagatgcg ttgatgcgcg caatcgattc
    10261 ggccctcgcg agagcccacg ttcgtcgcaa ggcggcggaa gatacggcgg cgcttcaggc
    10321 gctctatgag tcgctgacgc cgcgtgagca ggagcttttg ccgttactcg ccggcggcct
    10381 gctcaacaag caggcggcgg cagtactggg catcaccgaa tacaccgtgc aggtccatcg
    10441 cggccacatt atgagaaaaa tgcaggcgga ttcatttgcc gctctggtca ttctggcgag
    10501 caaactgcag ctcgaaacat cgtcggcggc cacagtcggt gcctgacgct accgctcact
    10561 cgttgtcggg cccgatgacc cggagaaaaa aatgtccacg cctccggttc gaaccgttgc
    10621 cgtgattgat gatgacaaac gtgttctaac ctctcttgca aacttgctgg cctctggcgg
    10681 gtacgaaacc cgggcctacg aatccgcact ggattttttc tccgatggct attcgggtct
    10741 gatatgcgtg attaccgacc tgggaatgcg gcccattgac ggcattcagg tgctcgatag
    10801 aacggtgcat tcggacgcag ccacgccggt catcattatc acgggcaaac cgggcgagca
    10861 tacggaaagc tactatctgc aaagaggcgc attgggtttt ttcagaaagc ctgtcgacgg
    10921 ggacgcattg ctggacctgc tggacagtat cccgtgatcg gttgatgtag tgaaccgggt
    10981 gcgtggggta gctggaaatc gctatggctc atgtgataga tgcgagcggc tagcggcaag
    11041 gtggcgcccg cgttgcatgc aacgccacgc cgaacgccgt gaaatgtgtc gaggcagcac
    11101 ggcgcggcaa cgagtccagc gctcgatttt accgagttgt ttcttgcggc ggcattgcat
    11161 attcatgtaa tgcggaatgc ctttttcggc gttattgtga cggcgtatat gcctccaggc
    11221 gctcggcgct cggcgctcgg cactcggcgc tcggcactcg gcactcgacg tgcggcgagc
    11281 ggagtgtggg ctgcctgaaa aggcgcgatc ggtttcttca gcgagcccct ggatgccagc
    11341 gcctttggcg cttctcgacg cgttgtaaag ctggcgtcgc ctctttcgtg cgcgcgaaca
    11401 cgtgacattg attccactca cagagacgac tttgaagcga cacatgccac ttgccggagg
    11461 cagaggttcg aagcgcgcaa tcgccacggc cacgattgcc gcgattgccg cgattccgat
    11521 gtcagggtgc accggttcgc cttccattgg cgtgctcggc gcgtactttc cggactggct
    11581 gttctgtatc gtcgcgggcg ttgtgctgac ggtggtcatc tatctgattc tcaagcgttt
    11641 gcaggccgat catctgctgg gacccgcggc cgtggtctat cccacactcg tcactttgct
    11701 cgcactcgct gcctggttga cgctgttcca acattgaatc tcgctcaggt ggatgaatgg
    11761 cgacgacagc cagcagcaca tcaaggagaa aatggccggc catcgtgctg gtcgtggtga
    11821 cgctgttgtt gctgctgttc gtcatccggc tgctggaccg tgcgccgcgc accgacgacg
    11881 cctacgtgta cgcggatacg atcgacgtcg tgcccgaggt caatgggcgc atcgtcgaac
    11941 tgcccgtgcg cgacaatcag gcggtgaaac agggcgatct gctgtttcgc atcgatccgc
    12001 gtccgtatca ggatgcgctg acgcggggca acgcgtccct cgtcgcgctc gaccggcaga
    12061 tcgaactgac ccagcgcacg gtcaatgcgc agcaatacaa cgcacagtcg gtgcgtgcgg
    12121 ccgtggaacg cgcgcgcgct gcggccagcc aggcggccga tacgctgcat cgcatggagc
    12181 cgcttttgtc aaaaggttat gtgtcggcgg aagacgtcga ccgtgcgcgc accgcacaac
    12241 gagccaccca ggccgaactc gttgccgcgc aactgcaggc gcagcaggcg gcagcggcgg
    12301 tgagcggcgt cgatgcgctg gtcgcgcaac gtgccgtcgt catggcggag atcgcgagtg
    12361 ccgaactgaa cctcgaatat gcaaccgtgc gcgctccgtt cgatggacgc atcgtctcgt
    12421 tgaaaacagc gacgggccag ttcgcgtcgg tactcaaacc cgtcttcacc ttgatcgaca
    12481 cgcgccactg gtacgtggtc gcgaactttc gcgagacgga actcaagggc atcggcgtgg
    12541 ggacgcctgc cacggtctac ctgatgagcg atacgggcca gcgcttccgg ggcagtgtcg
    12601 attcgatcag ctatggcgtc gcgccctctg aagccggcct tgcattgccg ggcggattgc
    12661 cgaacattca acgcaccctt aactgggtcc atgtatcgca gcgctttccg gtgaagatcc
    12721 gggtcgacaa tccgaacccg gcgctgttcc ggctcggtac gtcggcggtg gccgtgctgg
    12781 agcccgggcg cggcaacgac agcgagcgtc attgag
//
LOCUS       DATASH010000234         2436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4382453,
            whole genome shotgun sequence.
ACCESSION   DATASH010000234 DATASH010000000
VERSION     DATASH010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4382453"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /locus_tag="VHV99_06580"
     CDS             complement(<1..999)
                     /locus_tag="VHV99_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HEX3635143.1"
                     /translation="MSEPQHPQPQDTDTNEGHPAPVARAVVQPVVQPGLVDLDSLAAV
                     GARLTQLRESKGWTVEDVSARLKVSVTKLRALESGDISHLPDTTFALGVVRSYAKMLG
                     ADPTPFTQALRREKGVPAPDLSMPASSGKDLPRGRVSLSLGGSGQKSRSWLWGIAAVI
                     VAVIALTMWHTNGGDSSAWLARLKASANGAAGSATGASGAVAQGQAAGSEATAEDAAS
                     APETQAAVDNAASAMPMPAPLATGTAPSSAPAVTAAGVAPTAGAQAAAQGASAPVVAA
                     PGASAAAPAAGEAIVALRVTQDSWFSVRGKDGKEVFSGLVHAGDTKEVTGAAPFKVT"
     gene            complement(1218..2369)
                     /gene="rlmN"
                     /locus_tag="VHV99_06585"
     CDS             complement(1218..2369)
                     /gene="rlmN"
                     /locus_tag="VHV99_06585"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEX3635144.1"
                     /translation="MTSSPTVNLLDLDAQGLVAYCDSLGEKPFRAKQLQRWIHQYNAA
                     DFDGMTDLAKSLREKLKGRATITMPGIVSDNISTDGTRKWLIDVGNSNAVETVYIPEE
                     TRGTLCVSSQAGCAVNCRFCSTGKQGFSRNLTTGEIIGQLRMAEFALRATRGTDGGRA
                     TGGDGKGERVVTNVVMMGMGEPLLNYDAVVPAMRLMLDDNAYGLSRRRVTLSTSGVVP
                     MMDRLGADLPVALAVSLHAPSDPLRDMLVPLNKKYPLRELMAACQRYLKVAPRDFITF
                     ESCMLDGVNDSEAQARELLAVTRDVPCKFNLIPFNPFPESGLIRSKPEQIKRFAQVLM
                     DAGVVTTVRKTRGDDIDAACGQLAGAVKDRTRLAERTGKAAKVIEVRAV"
BASE COUNT          433 a          837 c          715 g          451 t
ORIGIN      
        1 cgtgaccttg aaaggcgccg cgcccgttac ttccttcgta tcgcctgcgt gcacgaggcc
       61 ggagaacact tccttgccgt ccttgccacg cacgctgaac caactgtctt gcgtcacgcg
      121 cagcgcgacg atcgcctcac ctgcggctgg tgccgcggcc gatgcgccag gcgcggcgac
      181 caccggcgcg ctcgcgcctt gcgccgcagc ctgcgccccg gccgttggcg caacccctgc
      241 agccgtcaca gccggtgcgg aagacggcgc cgtaccggtt gccagcggcg cgggcatcgg
      301 catagccgac gcggcgttgt ctactgcagc ctgcgtttcc ggcgcggaag cagcgtcttc
      361 cgcggttgcc tcggaacccg ccgcctgacc ttgcgccacc gcgcccgatg cgccggtcgc
      421 gctacccgcg gcgccgttcg cgctcgcctt caaacgtgcg agccacgccg acgaatcacc
      481 gccgttggta tgccacatgg tgagggcgat caccgcgacg atcaccgccg cgatacccca
      541 cagccacgag cggctctttt gcccactgcc gccgagcgac agcgatacgc ggccgcgcgg
      601 caaatcctta cccgacgaag ccggcatcga cagatccggc gcaggcacgc ccttctcacg
      661 gcgcagagcc tgcgtgaacg gcgtgggatc ggcgccgagc atctttgcgt agctgcgcac
      721 cacgccgagc gcaaaggtcg tgtccggcaa gtgactgatg tcgccggatt cgagcgcacg
      781 caatttggtg acggatacct tcaggcgagc cgacacgtct tcgaccgtcc agcccttcga
      841 ttcacgcaac tgggtcaagc gcgcgccaac cgctgccagc gaatccaaat ccaccaggcc
      901 cggctgcaca acaggttgca ccaccgcccg ggcgactggc gccggatggc cttcgtttgt
      961 gtctgtatcc tgcggctgcg ggtgctgcgg ctcactcatc ccaaatcctc tcgcgtcgat
     1021 tctttttcac ttgtgcaacc tggacgggcc aaagcctcac gcccgaatcg cagaccgttt
     1081 ataacaaaat gcgcggcctt atgtgccgct tccgatcgct tctgcaaact ttcttttcaa
     1141 tcgcggatcg cgcggagggt ttttgcgtgc ccggacagct ttcgtccgac cacctcgccc
     1201 taccccgcct cgcacgatta cacggcgcga acctcgatca cctttgccgc cttcccggtg
     1261 cgctcagcga ggcgcgtgcg gtctttcacc gcaccagcca actggccgca ggcagcgtcg
     1321 atatcatcgc cgcgtgtttt acgcactgtg gtgacaacgc ccgcgtccat caacacttgt
     1381 gcaaaccgct tgatctgttc cggcttcgag cgaataaggc ccgactcggg gaacggattg
     1441 aacggaatca ggttgaactt gcacggaacg tcgcgcgtca cggccagcaa ttcgcgcgct
     1501 tgcgcttcgc tgtcgttgac gccgtcgagc atgcaagatt cgaacgtaat gaaatcgcgc
     1561 ggcgcgactt tcagatagcg ctggcaagcg gccatcaatt cgcgcagcgg atacttcttg
     1621 ttcagcggaa ccagcatgtc gcgcaacgga tcgctcggcg catgcaacga gaccgcgagc
     1681 gccaccggca gatcggcgcc gagccggtcc atcatcggca ccacgccgga ggtggacagc
     1741 gtgacacgcc gacgtgacag gccatacgcg ttgtcgtcca gcatcaggcg catggcgggc
     1801 accaccgcgt cataattgag cagcggctca cccatgccca tcatcaccac gttcgtgacg
     1861 acgcgttcgc ctttgccgtc accacccgtg gcgcgcccgc catccgtgcc gcgcgtcgca
     1921 cgcagcgcga actcggccat gcgcaactgg ccgatgattt caccagtggt gagattgcgg
     1981 gagaaaccct gcttgccggt cgaacagaaa cggcagttga ccgcgcaccc ggcctgcgac
     2041 gacacgcata gcgtgccacg cgtttcttcg gggatgtata cggtttcaac cgcattgcta
     2101 ttaccgacgt cgatcagcca cttgcgcgtg ccgtcggtcg aaatattgtc gctgacgatg
     2161 ccgggcatcg tgatcgttgc gcgtcctttg agcttttcgc gcaaggactt cgccagatcg
     2221 gtcataccgt cgaagtcggc ggcgttgtac tgatgaatcc agcgctgcaa ttgcttggcg
     2281 cgaaacggct tctcacccag actgtcgcag taggcgacaa gcccttgggc gtcgaggtcg
     2341 agaaggttga cggtgggact gctcgtcata tcgaatcctg ccatttcagt gcgagactac
     2401 gttcatgcaa caagctgcat gaacgcaatg ccgttc
//
LOCUS       DATASH010000235         2291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1019998,
            whole genome shotgun sequence.
ACCESSION   DATASH010000235 DATASH010000000
VERSION     DATASH010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1019998"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(265..1734)
                     /locus_tag="VHV99_06590"
     CDS             complement(265..1734)
                     /locus_tag="VHV99_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3635145.1"
                     /translation="MASPDQTGPVARSAGGAASSATATAAASAASATLDAGSISARLD
                     RLPATRSIWKLVVLLSLGFFFELYDLLYSGYVAPGLVKSGLLTATTHGLFGSTGVASF
                     IAALFSGLFIGTIACGFLADRFGRRAVFTYSLLWYTAANVVMAFQETATGLNFWRFVA
                     GIGIGVELVTIGTYISELVPKQIRGRAFACEQAVGFTAVPVVAFLSYLLVPRTLFGLD
                     GWRWVVLIGAHGALFVWWIRRALPESPRWLAQQGRLAEADRVMSELEAKVRRESGREL
                     PLPAPPVPVAPRGSFRDMWVSPYRSRTLMMTIFNIFQTVGFYGFANWVPTLLIKQGVT
                     ITTSLMYSSVIALAAPIGPVIGLFIGDRFERKTVIVVMAAANIVCGLWFSQASGAVLL
                     VSLGVCLTLAGNIISYSFHAYQSELFPTSIRARAVGFVYSWSRFSAIFTAFLIAEVLK
                     QFGTSGVFVFIAGAMLIVMLAIGLMGPRTKGLELEKISK"
     gene            complement(1887..>2291)
                     /locus_tag="VHV99_06595"
     CDS             complement(1887..>2291)
                     /locus_tag="VHV99_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEX3635146.1"
                     /translation="QRNTSCTAGKREDFDFYHEVLRWGVEQGMHVPEGTRLFLQFGTM
                     AVRAYCESQGYLPVFERAGVTLVMPGCGSCANCGPGQSAEANQVTISAINRNFPGRSG
                     PGNVWLASPYTVAASALAGKITTFEQLKRTHG"
BASE COUNT          477 a          775 c          683 g          356 t
ORIGIN      
        1 actatctgac acggcagttg cagcctgtcg ccgatgcaat tctgccgcta ttgcgcgacg
       61 atttcacgac actgatgtcg gggcagaagc agctgttctg agccggtgtc agcgtcgcgc
      121 tcatcgctaa ccgataaacc agcgccgctc tgtcatttgc gattgccgtg acaacagccc
      181 ggcgcgaagg actcgaccgc tggcaatcgc aggcgaacaa ccgaactatg ccgaacgcac
      241 gacacagagg cccccccccg gcgactactt cgaaatcttc tccaactcga gccccttcgt
      301 acgcggcccc atcaggccga tcgccagcat cacgatcagc atcgccccgg cgatgaacac
      361 gaatacgccc gacgtgccga actgcttcaa cacctcggcg atcagaaagg ccgtgaaaat
      421 cgccgaaaac cggctccatg agtaaacgaa gcccaccgcg cgcgcacgga tgctggtcgg
      481 aaaaagctcg ctctgatagg cgtgaaagct gtacgaaatg atgttgcccg ccagcgtcag
      541 acatacgccg aggctcacca gcaacacggc gccggacgcc tgactgaacc acaggccgca
      601 cacgatattc gccgccgcca tcaccacgat caccgtcttg cgctcgaagc ggtcgccgat
      661 gaaaaggccg atcaccgggc cgatgggcgc ggccagcgcg atcacgctcg aatacatcag
      721 gctcgtggtg atcgtaacgc cctgcttgat caggagcgtc ggcacccagt tcgcaaagcc
      781 gtagaagccg accgtctgga agatattgaa aatcgtcatc atcagcgtgc ggctgcggta
      841 cggcgacacc cacatatcgc ggaagctgcc gcgcggcgcg accggcaccg gcggcgcggg
      901 tagcggcaat tcgcggccgg attcgcggcg cactttcgct tcaagctcgc tcatcacgcg
      961 atcggcttcc gcaagacggc cttgttgcgc gagccaacgc gggctttccg gcaaggcgcg
     1021 ccggatccac cacacgaaca gcgcaccatg tgcaccgatc agcaccaccc agcgccagcc
     1081 gtcgagtccg aacagcgtgc gcggcaccag cagataggac agaaacgcga ccaccggcac
     1141 cgcggtaaaa ccgaccgcct gctcgcacgc gaaagcgcgg ccgcgaatct gtttaggcac
     1201 cagttccgag atatacgtgc cgatcgtcac cagctcgacg ccgatgccga tgcccgccac
     1261 gaagcgccag aaattcagac ccgtggccgt ttcctgaaac gccatcacga cattggccgc
     1321 cgtgtaccac aacaacgaat acgtaaacac ggcgcggcgt ccgaaacgat ccgccagaaa
     1381 gccgcatgcg atcgtgccga tgaacagccc gctgaacaac gccgcgataa aactcgcgac
     1441 gccggtcgag ccgaacaggc cgtgcgtggt cgccgtcagc aaaccgcttt tgacgaggcc
     1501 tggcgcgacg tagcccgagt acagcaggtc gtaaagttcg aagaagaagc cgaggctcag
     1561 cagcaccacc agcttccaga tggaacgcgt ggcgggcagc cggtcgagcc gcgcggagat
     1621 gctgccggca tcgagcgtgg cggacgccgc agatgcagca gcagtcgcgg tggcggatga
     1681 agccgcgccg ccggcgctgc gcgcgacggg cccggtctga tcgggggatg ccatggtgtt
     1741 tgtctcctgg taatgcgggt catgctggcg ttgcacgatg atgcacgccg gggccggtcc
     1801 cgtgccggcc tttcgtatag tcgtactttc gtactttcgc gcgtgacgcg caccacgatg
     1861 ccgggccgca aggtccggcc agcattctag ccgtgtgtgc gcttcaattg ttcgaaggtg
     1921 gtgatttttc cggccagcgc gctcgccgcg acggtgtacg ggctcgccag ccacacattg
     1981 cccggccccg aacggccggg gaaattgcgg ttgatcgcgc tgatcgtcac ctggttcgcc
     2041 tcggccgatt gccccggtcc gcaattcgcg cacgagccgc agcccggcat cacgagcgtc
     2101 acaccggcgc gctcgaacac cggcaaatag ccctggctct cgcaatacgc gcgcaccgcc
     2161 atggtgccga attgcagaaa gagacgcgtg ccctcgggca catgcatgcc ctgctccacg
     2221 ccccagcgca atacctcgtg atagaagtcg aagtcttcgc gcttgcccgc cgtacacgag
     2281 gtattgcgct g
//
LOCUS       DATASH010000236         5679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2997198,
            whole genome shotgun sequence.
ACCESSION   DATASH010000236 DATASH010000000
VERSION     DATASH010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5679
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2997198"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="VHV99_06600"
     CDS             complement(<1..406)
                     /locus_tag="VHV99_06600"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III glutamate--ammonia ligase"
                     /protein_id="HEX3635147.1"
                     /translation="MADPDGLVRRMTEQLDAIGLDVFAVNHEFMNSQYEINLRHSSAL
                     DAADRAFRLKSAVKDIAAINGLHATFMGKPFNDQGGSGTHLHLSLRRDDANIFDAPSA
                     EHGVSQELRQFSAGLIEHAAALMALLNPTINAY"
     gene            571..1809
                     /gene="glp"
                     /locus_tag="VHV99_06605"
     CDS             571..1809
                     /gene="glp"
                     /locus_tag="VHV99_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEX3635148.1"
                     /translation="MLTTAEAMATLLSAAHPINGTESIPTLDALNRVLSADVISPLDV
                     PPMNTSSMDGYAIRTADLTAQDNRSLPISQRIPAGHAPEPLKPGTAARIFTGATVPPG
                     ADAIVMQEQTEAAGNEVTILHSPTPGEWITAQGADIRSGATILPAGTRLTPQALGLAA
                     SVGCAELEVRHRVKVAVFFTGDELTMPGEPLKPGAIYNSNRFTLRGLLEKLGCEVTDY
                     GIVPDKLDATRATLREAAQAHDLILTCGGVSVGEEDHVKPAVEAEGRLSMWQIAMKPG
                     KPLAFGAVRRDAQQTDAAGEAAGETFFIGLPGNPVSSFATFLLFVRPFVLLLAGMQTV
                     APRALSLRADFTQSKADRRNEFLRARINPAGGLDLFPNQSSAVLTSTVWGDGLIDNPP
                     NHAISAGETVRFIPFSELLN"
     gene            1836..2093
                     /gene="moaD"
                     /locus_tag="VHV99_06610"
     CDS             1836..2093
                     /gene="moaD"
                     /locus_tag="VHV99_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010115001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HEX3635149.1"
                     /translation="MKIQLRYFASVREALETADETVDVPDGIATVGDVRSWLRARGGI
                     WADTLADGRALRMACNHVMTNAGTRITEGCEVAFFPPVTGG"
     gene            2198..2674
                     /gene="moaE"
                     /locus_tag="VHV99_06615"
     CDS             2198..2674
                     /gene="moaE"
                     /locus_tag="VHV99_06615"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HEX3635150.1"
                     /translation="MPVRVQTEDFDLTAEVAALRARNPKIGAVACFVGTVRDLNDGSA
                     VETMELEHYPGMTEKSLEAIVVSARERWPGIEVLIVHRIGKLYPLDQIVLVATTAAHR
                     GDAFASCEFVMDYLKTQAPFWKKEKTEEGERWVDARVADDAALARWGVESGNQAED"
     gene            complement(2783..3328)
                     /locus_tag="VHV99_06620"
     CDS             complement(2783..3328)
                     /locus_tag="VHV99_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HEX3635151.1"
                     /translation="MNPSFPAAPFVERAAEPTEANIRELVHGFYDRVRADPLLGPVFD
                     ATLAGRWDDHLPKMCTFWGSLVLGAKQYRGNVQQAHQPLEGVEPQHFSRWLYLFLDTV
                     ESRYEPAAAVRFMEPALRIAQSLQLSRFGWDYKIPAEQQALLARVAPKRHPREDEDSH
                     DATRPRGEPFPTRIVGKARGE"
     gene            3632..4123
                     /locus_tag="VHV99_06625"
     CDS             3632..4123
                     /locus_tag="VHV99_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX3635152.1"
                     /translation="MRLTDYTDYALRVLLYLAVRGEGLSTIQDISDAYGISKNHLMKV
                     VQQLGERGWVETVRGRNGGLRLVEQTNALTVGEIVRATESDFALVGCFPDQQGERRAC
                     VITAHCRLRGALEAARNAFLAELDRHTIREVAGPHGPLAALLGLSTVIPIMPADDGAA
                     PLT"
     gene            4243..>5679
                     /locus_tag="VHV99_06630"
     CDS             4243..>5679
                     /locus_tag="VHV99_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEX3635153.1"
                     /translation="MRIDKLTTKFQEALADAQSLAVGHDNQYIEPVHVLSALVAQQDG
                     AARSLLSRAGVHVQALQTALNDAMTRLPQVQGTDGNVQIGRELTGLLNQADKEAQKLN
                     DTFIASEMFLLAVADDKGEAGRLARQHGLSRKSLESAIVAVRGGSQVHSQDAESQREA
                     LKKYTVDLTERARAGKLDPVIGRDDEIRRSIQILQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPETLKGKRVLSLDMAALLAGAKYRGEFEERLKAVLSDIAKDEGQTIVF
                     IDEIHTMVGAGKAEGAMDAGNMLKPALSRGELHCVGATTLDEYRKYIEKDAALERRFQ
                     KVLVDEPSVEATIAILRGLQEKYELHHGVDITDPAIVAAAELSHRYITDRFLPDKAID
                     LIDEAASKIKMEIDSKPEEMDRLDRRLIQLKIEREAVKKEKDEASQKRLQLIEEEIER
                     LNREYSDLEEIWTAEKAAVQGSAQLKEEI"
BASE COUNT         1183 a         1802 c         1752 g          942 t
ORIGIN      
        1 ggtaggcatt gatcgtcggg ttgagcagcg ccatcagcgc tgcggcgtgc tcgatcaggc
       61 cggcggagaa ctggcgcagc tcctggctga caccgtgctc tgcggacggg gcatcgaaga
      121 tgttggcgtc atcgcgcctc agcgagaggt gcagatgggt cccagagccg ccctggtcgt
      181 tgaacggctt gcccatgaag gttgcgtgca gcccgttgat ggcggcgatg tccttcacgg
      241 cgctcttcag acggaacgcg cggtccgcgg cgtccagcgc gctcgaatgc cgcaggttga
      301 tctcgtactg cgagttcatg aactcatggt tgacggcgaa aacatccagg ccgatcgcgt
      361 cgagctgctc ggtcatgcgg cgcacgagcc cgtcgggatc ggccatcggg ccaacggtgt
      421 agaccaactc ctgcaggccc ccaaaaaatc accaccgccg aaggcaaaac ccccaggccc
      481 ccaccgccca tcatcatcgc ccgctacaat attcctttaa aacccgcggc tccgagacta
      541 aagatgtcca ccccaacgcc ccgcgctccg atgctcacca ccgctgaagc catggcgacg
      601 ttactgagcg cagcgcaccc gatcaacggc acggaatcga tccccacact agacgcgctg
      661 aatcgcgtgc tatcggcaga cgtcatttcc ccgctcgacg tccccccaat gaacaccagt
      721 tcaatggacg gctacgcgat ccgcacagcg gacctgacag cgcaagacaa ccgcagtctg
      781 ccaatttccc aacgcatccc agccggccac gcacccgagc cgttaaaacc aggcacggca
      841 gcccgtatct tcacaggcgc cacagtgccg ccgggcgccg acgcgatcgt catgcaggag
      901 caaaccgagg ccgccggcaa cgaagtcacc atcctccaca gcccgacccc gggcgaatgg
      961 atcacggccc aaggcgccga catccgcagc ggtgcgacca ttcttccagc gggcacgcgc
     1021 ctgacaccgc aagccttggg gctcgccgcc tcggtcggct gcgccgagct cgaagtgcgc
     1081 caccgcgtaa aagtcgccgt gttcttcacc ggcgacgaac tgaccatgcc aggcgagccg
     1141 ctgaaacccg gcgcgatcta caactcgaac cgtttcacgc tgcgcgggct gctggaaaaa
     1201 ctgggctgcg aggtcaccga ttacggcatc gtgcccgata agctcgacgc tacccgcgca
     1261 acactgcgcg aagccgcgca agcacacgat ctgatcctga cctgcggcgg cgtctcggtt
     1321 ggcgaggaag atcacgtcaa gccggcagtc gaggcggaag ggcgcctgtc gatgtggcag
     1381 atcgcgatga aacccggcaa gccactcgcg ttcggcgcag tgcgccgcga cgcgcagcaa
     1441 accgacgccg ccggggaagc agcgggtgag accttcttca tcggcctgcc tggcaacccg
     1501 gtctccagct tcgcgacctt tttgctgttc gtgcgcccgt ttgtcctgct tctggccggc
     1561 atgcagacgg tcgcaccgcg cgcactgtcg ctgcgagcgg atttcacgca gagcaaggcc
     1621 gaccgccgca acgaattcct gcgcgcacgg atcaatccgg ccggtggcct cgatctgttt
     1681 ccgaatcaga gttcggccgt gctgacttcg accgtatggg gcgacggcct gatcgacaat
     1741 ccgccgaacc acgcgatcag cgccggcgag accgtgcgct tcatcccctt ttccgaattg
     1801 ctcaactgag gccggcaata gccggcggct tcccgatgaa gattcaactc cgatattttg
     1861 caagcgtgcg tgaagcgctc gaaacagccg acgaaacggt cgacgtgccc gacggcatcg
     1921 ccaccgtcgg cgacgtgcgc tcctggttgc gcgcgcgcgg cggcatctgg gccgacaccc
     1981 tcgccgacgg ccgcgcgctg cgcatggcgt gcaatcacgt catgaccaac gccggtaccc
     2041 ggattaccga aggctgcgaa gtcgcgttct ttccgcccgt caccggcggc tgagtggcgc
     2101 cgcatagtcg ctaaacacca cggccgtata gtcgctaaac actacgttga gtctcgccaa
     2161 acaggtcgag cctcgccaaa ccgcacagga gccagagatg cccgttcgcg tccagacgga
     2221 agacttcgac ctcaccgccg aggtcgccgc gttgcgcgcg cgcaatccga agatcggcgc
     2281 agtggcctgt tttgtgggca ccgtgcgcga tctgaacgac ggcagcgccg tcgaaaccat
     2341 ggagctcgaa cactacccgg gcatgacgga gaagtcgctg gaggcgatcg tcgtgagcgc
     2401 gcgtgagcgt tggcccggga tcgaggtact gatcgtgcat cggattggca agctttatcc
     2461 actcgaccag atcgtgctcg tggcgaccac cgccgcccat cggggcgacg ccttcgcgtc
     2521 gtgcgagttc gtgatggact atctgaagac ccaggcgccg ttctggaaga aagagaagac
     2581 cgaagaaggc gagcgctggg tcgatgcacg tgtcgcggac gacgcggcgc tggcccgttg
     2641 gggcgtcgaa tcggggaatc aggctgagga ttagcgcgcg tgcttgtgct cgcggcatgc
     2701 ggtccttctg gcgaccacgg aatggcgatg tagcgctgga tgatcgcctg ttgaacgggc
     2761 cagagccttt aaagcaagcc cgtcactcgc cccgcgcttt gccgacgatc cgcgttggaa
     2821 acggctcgcc gcgtggtcgc gtggcgtcgt gtgaatcctc atcctcgcgc ggatgccgct
     2881 tcggtgcgac gcgcgccagc aaggcctgtt gctccgccgg aatcttgtaa tcccagccga
     2941 agcggctcaa ctgcaaactt tgcgcgatcc gcagcgccgg ttccatgaag cggaccgccg
     3001 cggccggctc gtagcgcgac tccactgtat ccagaaacag atagagccag cggctgaaat
     3061 gctgcggctc gacgccctcg agcggctgat gcgcctgctg cacattgccg cggtattgct
     3121 tcgcgccgag cacgaggctg ccccagaacg tgcacatctt gggcaagtgg tcgtcccaac
     3181 ggcccgccag cgtcgcgtcg aacacggggc ccaacagcgg atcggcccgc acacggtcgt
     3241 aaaagccgtg gacgagttcg cgaatattcg cctcagtagg ctcggcggcg cgctcgacaa
     3301 acggtgcggc agggaaagac ggattcatga agttccaggt cgtgcggtta ttcatcagga
     3361 atcgcgccca cagcgcgatc gacagtcaga aacgacctca aactgtgtcc aaacaaaagg
     3421 aagtaggccg cgaataccct gcttttcgta agaatggcct aagggggaaa cgttaccatt
     3481 gctgccgaaa gacaaacacc gactgtcagc gcagaataag aaaacatgcg caacgcccgc
     3541 atcttagggc gaagcgacag ctcccggcaa cctttgcgtc gacgcggtta tccccggatc
     3601 gcggcttgcg ctcatcgtga cacctgcact catgagactg accgactata cggattacgc
     3661 actacgtgtt ctcttgtacc tggccgtccg tggcgagggc ttatcgacca tccaggacat
     3721 ctcggacgcg tacggcatat ccaagaatca cttgatgaag gtggtgcagc aactcggcga
     3781 acgcggctgg gtcgagaccg tacgcgggcg caacggcggc ctgcggctcg tcgaacaaac
     3841 caacgcattg accgtgggcg agatcgtgcg ggccacggaa agcgactttg cgctggtcgg
     3901 ctgcttcccg gatcagcaag gtgagcgccg ggcctgtgtc attacagcgc actgccgtct
     3961 gcgcggcgca ctcgaggcgg cgcgcaatgc gttcctcgcc gaacttgacc gccatacgat
     4021 ccgcgaggtt gcggggccac atggcccgct ggctgcgctg ctcggtttaa gcaccgtcat
     4081 cccgatcatg cctgccgacg acggcgctgc gccgctgacc taaaagcggc cagcaatttt
     4141 tcgcgcgtca aatgtcaaat agttgttgtc tgctgcaaaa aagcgcttga aacctgcgta
     4201 aaacgcctca ttttggtggt aatcgaaatt ggaggtctct tgatgagaat cgacaaactt
     4261 accactaaat tccaggaagc actggccgac gcgcagagtc tggctgtcgg tcatgacaat
     4321 caatacatcg aaccggttca tgtcctgtcg gcgctcgtcg cccagcagga cggcgcggcg
     4381 cgttcgctgc tctcgcgcgc gggcgtgcat gtccaggcgt tgcaaacggc cctgaacgat
     4441 gccatgacgc gcctgccgca agtgcagggc accgacggca acgtgcagat cggccgcgaa
     4501 ctgaccggct tgttgaacca ggccgacaag gaagctcaga aacttaacga cacgttcatc
     4561 gcgagcgaga tgttcctgct cgcggtcgcc gacgataagg gcgaggccgg ccgtctcgcg
     4621 cgtcagcacg ggctgtcgcg caagtcgctg gaaagcgcga tcgtggcggt gcgcggcggt
     4681 tcgcaagtgc atagccagga cgccgaaagc cagcgcgaag cgctgaagaa atacaccgtc
     4741 gatctgaccg agcgcgcgcg ggcgggcaag ctcgaccccg tgatcggccg tgatgacgaa
     4801 atccgccgtt ccattcagat cctgcagcgc cgcaccaaga acaatccggt gctgatcggc
     4861 gagccgggcg tgggtaagac ggcgatcgtc gaaggcttgg cgcagcgtat cgtcaacggc
     4921 gaagtgccgg aaacgctcaa gggcaaacgc gtgttgtcgc tggacatggc ggcgttgctg
     4981 gcgggcgcca agtatcgcgg cgaattcgaa gaacgcctga aggccgtact gagcgacatc
     5041 gcgaaagacg aaggccagac catcgtcttc atcgatgaaa ttcataccat ggtcggcgcg
     5101 ggcaaggcag aaggcgccat ggacgcgggc aatatgctca agcccgcgct ctcgcgtggc
     5161 gaactgcatt gcgtcggtgc caccacgctc gacgaatatc gcaagtacat cgaaaaggac
     5221 gccgcgctcg aacgccgctt ccagaaggtg ctggtcgacg agccgtcggt ggaagccacc
     5281 atcgcgatcc tgcgcggttt gcaggagaag tacgaactgc atcacggcgt cgatatcacc
     5341 gacccggcga tcgtcgccgc ggccgaactg tcgcatcgct acatcacgga tcgctttctg
     5401 ccggacaagg cgatcgatct gatcgacgaa gccgcctcga agatcaagat ggaaatcgac
     5461 tccaagccgg aagagatgga tcggctcgac cgtcgtttaa tccagttgaa gatcgaacgc
     5521 gaagccgtca agaaggaaaa agatgaagca tcgcaaaagc gtctgcaact gatcgaagaa
     5581 gaaatcgagc ggctgaatcg tgaatattcg gacctcgaag aaatctggac cgcggaaaaa
     5641 gcagcggtgc agggcagcgc gcagttgaag gaagagatc
//
LOCUS       DATASH010000237        10972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3987289,
            whole genome shotgun sequence.
ACCESSION   DATASH010000237 DATASH010000000
VERSION     DATASH010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 10972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10972
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3987289"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3884)
                     /locus_tag="VHV99_06635"
     CDS             complement(<1..3884)
                     /locus_tag="VHV99_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nitrate reductase/sulfite reductase
                     flavoprotein subunit alpha"
                     /protein_id="HEX3635154.1"
                     /translation="MSSNNVKTVCPYCGVGCGMVLHVEDGHVVKISGDKEHPANFGRL
                     CTKGQSAHVALRKSGRLEGAFVRHARDQDPVPLPMAQAISATAMRLRGLLDEHGADAL
                     SFYVSGQMSIEAQYLVNKLAKGFVGTNNIESNSRLCMASAGSGYKLSLGADGPPGSYD
                     DIDHADLFFVTGANMADCHPILFLRMMERVKAGAKLIVVDPRRNTTAEKADLFLPIKP
                     GTDLALLNGLLHLLHENGHTDTSFIAEATEGWDAMPAFLADYTPERVSEITGLAVADI
                     REAARMIGAAPAWISCWTMGLNQSTHGTWHTNAICNLHLATGKICRRGSGPFSLTGQP
                     NAMGGREMGYMGPGLPGQRSVLIEEDRAFVEDLCGIPNGTLRTEVGNGTIDMFARMAA
                     GEIKACWIICTNPVASLANRQNAIAGLRAAELVISQDAFLDTETNRYADVLLPGALWA
                     EAEGVMINSERNLTLMQKGIEPPGAALPDWQIIARVACEMGFAAAFTYASAEEVFAEI
                     TRASNPKTGYDLRGASHRRLKETPLQWPLAAADGVDRNPVRYLNDGVSQTLKERSDGS
                     RPRLVFPTASGKAVFFARDFAAPAELPDREFPIVLNTGRLQHQWHTMTKTGKVAMLNK
                     LNPGPFVEIHPEDAAALGIKAKDPVEIRSRRGRVVLPAIVTERVQAGNCFAPMHWNDV
                     FGDDLCINAVTSDAIDPVSQQPELKFCAVALSPVALEALAPVANAGDETFTDASAGAT
                     AAAPARAISTKSEELEMPRIDALTSLLQLARTPAPSLTDAERLYLAGFVSGLRSSEAP
                     GIDSVPVLPAGAPFDPTSRLYVDGLLAGLYSRSGTGAAKPAGSIAAPPQSAAAQAAAV
                     RIVRARPKVTLLWASQTGNTESLTERYATRLMESGFEIRTSCMADYQASALAKAQYVL
                     LMSSTFGDGDPPDNAQGFWSQLAGDAAPRLDGLRYAVLALGDRNYDQFCGHGRRLDER
                     FAAQGALRLMDRVECDSEYQAGADAWLERIIVRINEEDAALHAVPAGGMINAVVPGAV
                     PTKTRPASSRLVTNLQLNKQGAAKDTRYFSLSTGDSGLEYEAGDALGVWPSNCPELVD
                     ELISLSGVSADAAVSVPGAGDMRLAEALAKHYEIARPNPDALAFIAARTRNGALRDLL
                     SAERKADLKQWLWGQQLADVLHEFPVNLTATELTGMLKRLQPRLYSIASSPKVHPGEV
                     HLTVSAVRYNNGRRHRKGVSSTFLADRAGDANVPVFVQKSAHFRPPHISGTPIIMVGP
                     GTGVAPFRGFLHERRARGDTGRNWLFFGEQHAA"
     gene            complement(3927..4280)
                     /gene="nirD"
                     /locus_tag="VHV99_06640"
     CDS             complement(3927..4280)
                     /gene="nirD"
                     /locus_tag="VHV99_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HEX3635155.1"
                     /translation="MNLDRLPLVWTPICPLDEIVPNTGVCALVNGEQVAVFHVNTGDA
                     DGAVYAIENYDPGSHAAVLSRGLIGSLGERLVVASPIYKHHFDLRTGECLETPAYSVS
                     AFAARVENGQVWIAV"
     gene            complement(4317..6866)
                     /gene="nirB"
                     /locus_tag="VHV99_06645"
     CDS             complement(4317..6866)
                     /gene="nirB"
                     /locus_tag="VHV99_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HEX3635156.1"
                     /translation="MRIIVIGHGMVGHKLVECLAQDAAHGLDITVLCEESRPAYDRVH
                     LSEFFSGKTADDLSLVEAGFFERQNMRLLLNAKAVSIDRDTRTVTVSTGQTYDTLPYD
                     KLVFATGSYPFVPPVPGRERADCFVYRTIDDLEAMQECGTRSKTGTVVGGGLLGLECA
                     KALRDMGLQTHVVEFAPRLMAVQVDDGGGRVLRSKIEELGVTVHTQKNTTAIVDGENG
                     THRMQFADGSFLDTDMIVFSAGIRPRDDLARESGLTLGARGGIVIDNACRTSDPHIYA
                     IGECALWNGQLFGLVAPGYDMARAVAKQLLGDPAEFAGADMSTKLKLMGVDVASIGDA
                     HGNAPGSRAYQFSDERKQVYKKLVVSECGKYLLGGVMVGDASEYGTLLQMMLNRIELP
                     ASPEFLILPSSDGQAKPALGVEALPAGAQICSCNNVSKGDLCAAVRAGATDIGALKSA
                     TSAGTSCGGCVPLVTQVMKAEMKKQGMAVNNHVCEHFPYSRQELYHLVRVEGVRSFGE
                     LLAKHGHGLGCDICKPVVASILASCWNEFVLKKEHASLQDTNDYYLANIQRDGTYSVV
                     PRMAGGEVTPDGLIAVGQVAKKYGLYTKITGGQRVDLFGARVEQLPLIWEELIAAGFE
                     SGHAYGKSLRTVKSCVGSTWCRYGVGDSVGLAVDLENRYKGLRTPHKIKFGVSGCARE
                     CAEAQGKDVGIIATEKGWNLYVCGNGGMKPRHAELLASDLDKDTLVRYIDRFLMFYVR
                     TADRLQRTSVWRDNLEGGLDYLIDVIVNDRLGVAAELEGEMQHVVETYECEWKKAVTD
                     PETRKRFRHFVNSGESDSNVKFVEERGQIRPATLTERQSKLAAIPVVVETV"
     gene            complement(7472..8680)
                     /locus_tag="VHV99_06650"
     CDS             complement(7472..8680)
                     /locus_tag="VHV99_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEX3635157.1"
                     /translation="MNKLPLGDDEAAQITRDIETIRRIDAVPSILRLICKNTGMGFAA
                     VARVTEQSWTACAVQDEVNFGLAAGGQLDLHTTLCFESRAARASIVIDNFGNDPVYKG
                     HHTARIYNLGSYISVPIVLPDGSYFGNLCAIDPNPNEISDARTLSMFEVFAELIALQI
                     ATEGRQQAAEEALSREREISSLREQFIAVLGHDLRNPLSAVSATAELLALRKNEPDLV
                     KIGQRLKTTTFRMARLIDDVMDFARGRLGSGIGVSIDDVDDLAMALRVVIAESREANP
                     ERMLVDDIAIGTRVRCDRTRVQQLLSNLLGNAVTHGSAAFPVVVQARVEGSEFVLAVS
                     NGGNAIAPDALNRVFEPYWRPPTSKPGGGLGLGLYICKQIVSAHGGALEVRSSVEDGT
                     VFVARLPVGI"
     gene            8793..9173
                     /locus_tag="VHV99_06655"
     CDS             8793..9173
                     /locus_tag="VHV99_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3635158.1"
                     /translation="MSTDDAARLLIADDDPNLLAAYVLFFSAHGFDVRTARNGLDALA
                     QYCAWHPHAALLDVEMPRLDGRAVARRIRYVADTPAPMLVAVTGLACDEDRNESLRSG
                     FNHHFVKPVPMPVILAALTGRAWH"
     gene            complement(9288..10163)
                     /locus_tag="VHV99_06660"
     CDS             complement(9288..10163)
                     /locus_tag="VHV99_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HEX3635159.1"
                     /translation="MPTISELFVYPIKSCAGIALSEARLLATGLEYDRCWMVTEPSGA
                     MLTQRAYPRMALVKVELGEHELVIRAPGMSALRTPLEAAGLGAARKVQTAVWRDAAYG
                     LDTGEQSAAWFSDFLGVPARLLRFDPERERIVDPDYTGSVGGATTHFADGFPLLVVGQ
                     ASLDDLNVRLNNKGAPSIPIDRFRPNVVLTGLDAYEEDYVESLSVDGAEGAAVQLQLV
                     KLCSRCPMPTIDQAKGAPDPDWPNEPTDTMSVYRASPRRNGALTFGNNAIVARGAGAR
                     LRVGQAVEAELGFGD"
     gene            complement(10329..>10972)
                     /gene="glcC"
                     /locus_tag="VHV99_06665"
     CDS             complement(10329..>10972)
                     /gene="glcC"
                     /locus_tag="VHV99_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HEX3635160.1"
                     /translation="NSLSPSRTALREGLKLLRARGIIETSHGRGSFVASLTAQPTLTP
                     MMHLLGSQPRTLYDLFEVRALLEAESARLAALRGTAADFVLITRRYEEMLAAHGTETS
                     ASTHARLDHAFHLAICEASHNPVLVHTWTTLTDLLLSSVFASVNNLYHREPQKRVIDR
                     QHARLYNAVTGKLPEQARKAAADHIESVCANLREIEQEEQRLVRATLRLEGWE"
BASE COUNT         1969 a         3534 c         3436 g         2033 t
ORIGIN      
        1 gccgcatgtt gttcgccgaa gaacagccag ttgcgtcccg tgtcgcctct tgcacgccgc
       61 tcgtgcagaa agccgcgaaa cggtgcaacg ccggtgccgg gaccgaccat gatgatcggc
      121 gtgccggaaa tatgaggagg ccgaaagtgc gcggatttct gcacgaatac cggcacattc
      181 gcgtcgccgg cacggtcggc gagaaacgtc gacgacacgc ccttgcgatg gcggcggcca
      241 ttgttgtagc gaaccgcgga aaccgtcaga tgaacttcgc cgggatgcac tttcgggctc
      301 gaggcaatcg aatacaaccg cggttgcagg cgcttgagca tgccggtcaa ttcggtggcg
      361 gtcaggttca ccgggaattc gtgcagcacg tcggcgagct gctggcccca caaccattgc
      421 ttgaggtcgg ctttgcgttc cgctgagagc agatcgcgca gtgcgccgtt gcgggtacgc
      481 gcggcaatga acgcgagcgc gtcagggttg ggacgggcga tctcgtagtg tttggcgagc
      541 gcctcggcga ggcgcatgtc gcccgcgcca ggcacgctga ccgcggcatc ggcgctcaca
      601 ccgctcaggc tgatcaattc gtcgaccagt tcgggacaat tgctcggcca cacgcccagt
      661 gcatcgccgg cttcgtattc gaggcccgag tcgcctgtgc tcaacgagaa gtagcgcgta
      721 tccttcgcgg cgccctgctt gttcagctgc aggttcgtga cgagccgcga gctggccgga
      781 cgcgttttgg tcggcacggc gccgggcacc acggcgttga tcatgccgcc cgccggcacc
      841 gcgtgcagcg ccgcatcttc ttcgttgatg cgcacgatga tgcgttcgag ccacgcgtcg
      901 gcgcctgcct gatactcgct gtcacactcg acgcgatcca tcagacgtag cgctccttga
      961 gccgcgaaac gctcgtcgag ccggcggccg tgtccgcaga actgatcgta gttccggtcg
     1021 cccaacgcga gcaccgcata gcgcagaccg tcgagacgag gcgctgcatc gccggccagt
     1081 tgggaccaga aaccttgcgc gttatcgggc ggatcgccgt cgccgaatgt gctcgacatc
     1141 agcagcacgt attgcgcttt ggcgagcgcc gacgcctgat agtccgccat gcatgacgtg
     1201 cggatttcaa aacccgactc catcagacgc gtggcgtagc gttcagtgag cgattcggtg
     1261 ttgccggttt gcgaggccca taacaaggtc actttgggcc gcgcgcgcac gatgcgcacg
     1321 gcggccgcct gcgccgcggc ggactgcggc ggcgccgcta tcgaacccgc tggcttggcc
     1381 gcacctgttc cactgcggct atacagtccg gccaacagac catccacgta aagacggctg
     1441 gtcggatcga acggtgcgcc ggccggcagg accggcacgc tgtcgatgcc cggtgcctca
     1501 gaagaacgca ggccgctaac gaacccggcg aggtacaagc gctcggcatc cgtgagcgat
     1561 ggcgcgggtg tccgcgcaag ctgcaacaga ctcgtcaggg catcgatgcg gggcatttcc
     1621 agttcctcgg acttcgtcga tatcgcgcgc gccggcgcag cggcagttgc cccagctgat
     1681 gcatcggtga atgtctcgtc gccggcattc gcaaccggcg cgagggcttc gagcgcgacc
     1741 gggctcagcg cgactgcgca gaacttgagt tcgggctgct gcgagaccgg gtcgatggca
     1801 tcgctcgtca cggcgttgat gcacaggtcg tcgccgaaca cgtcgttcca gtgcatcggc
     1861 gcgaagcagt tgccggcttg cacgcgctcc gtgacgatcg ccggcaatac gacgcggccg
     1921 cggcgcgaac ggatctcgac tggatctttt gccttgatgc cgagcgcggc ggcgtcttcg
     1981 ggatgaatct cgacgaacgg gccagggttg agcttgttga gcatcgccac cttgccggtc
     2041 ttggtcatgg tgtgccattg atgctgcaag cggcccgtgt tcagcacgat cggaaattcc
     2101 cgatcgggca actcggccgg ggctgcaaag tcacgagcga agaacaccgc tttgccgctt
     2161 gcggttggaa acacgagccg cggacggctg ccgtcggagc gttccttcag cgtctggctg
     2221 acgccgtcgt tgaggtagcg aaccggattg cggtcgacgc cgtcagcggc ggccagcggc
     2281 cattgcagcg gcgtttcctt cagccggcga tggctcgcgc cgcgcaagtc gtagcccgtt
     2341 ttcggattcg acgcacgggt gatctcggcg aagacttcct cggcgctcgc ataggtaaac
     2401 gcagcggcga accccatttc gcaagcgacg cgcgcgatga tctgccagtc cggcaacgct
     2461 gcgcccggcg gctcgatacc cttttgcatc agcgtcaggt tgcgctcgga gttgatcatc
     2521 acgccttcgg cctcggccca cagcgcgccg ggcaatagca cgtcggcgta acggttggtc
     2581 tcggtgtcga ggaaggcgtc ctgcgaaatc acgagttcag cggcgcgcaa accggcgatc
     2641 gcgttctgac ggttcgcgag gctcgccacc ggattggtgc agataatcca gcacgccttg
     2701 atctcccccg cggccatgcg ggcaaacata tcgatcgtgc cgttgccaac ttcagtcctc
     2761 agcgtgccat tcggaatgcc acacaggtct tcgacaaacg cgcggtcttc ttcaatcaga
     2821 accgaacgct gtcccggcaa accgggcccc atgtagccca tttcgcggcc acccatcgcg
     2881 ttgggctggc cggtcaacga gaagggcccg ctgccgcggc ggcagatctt gcccgtcgcc
     2941 agatgcagat tgcagatcgc gttggtgtgc cacgtgccgt gcgtgctttg gttcaggccc
     3001 atggtccagc agctgatcca cgccggtgcg gcgccgatca tgcgcgcggc ctcacggatg
     3061 tcggcgacgg cgaggcccgt gatttccgac actctctccg gcgtgtagtc cgccagaaaa
     3121 gcaggcatcg cgtcccagcc ttcggtcgcc tcggcgataa aagacgtatc cgtatggcca
     3181 ttttcgtgca acagatgcag caggccattg agcaatgcca ggtcagtgcc cggcttgatc
     3241 ggtagaaaga gatcggcctt ctcagcggtc gtgttgcggc gcggatccac cacgatcagt
     3301 ttcgcccccg ccttcacgcg ctccatcatc cgcagaaaca ggatcgggtg acagtccgcc
     3361 atattcgcgc cggtcacgaa gaacagatcg gcatgatcga tatcgtcgta cgatcccggc
     3421 gggccgtccg cgccgagcga gagcttgtag ccgctacccg cgctcgccat gcacagacgc
     3481 gaattcgatt cgatgttgtt ggtgccgaca aaacccttgg cgagtttgtt gacgaggtac
     3541 tgcgcctcga tcgacatctg gccggacacg taaaacgaca acgcgtccgc tccatgttcg
     3601 tcgagcaggc cgcgcaaacg catcgccgtg gcgctgatcg cctgcgccat cggcagtggc
     3661 acgggatcct ggtcacgtgc gtgacgcacg aacgcgcctt ctaaacgccc cgacttgcgt
     3721 aacgcgacat gcgccgattg gcccttcgtg cagagccgcc cgaaattcgc cggatgctcc
     3781 ttgtcgccgg aaatcttcac gacgtgaccg tcctcgacgt gcagcaccat gccgcagcca
     3841 acgccgcagt aggggcagac agtctttacg ttattggagg acatggccga tggcatggtg
     3901 ggaagaagtt gcaaacaatc agtcgatcaa accgcgatcc acacctggcc gttttccacc
     3961 cgcgcggcga acgcactgac ggaatacgcc ggtgtctcga gacactcacc ggtgcgtaaa
     4021 tcgaagtgat gcttgtagat tggcgaagcg acgacgaggc gctcgccgag actgccgatc
     4081 agcccgcgcg agaggactgc cgcatgcgag cccggatcgt agttttcgat tgcatacacg
     4141 gcgccgtccg cgtcgccggt gttcacatga aagacggcca cctgttcgcc gttgacgagc
     4201 gcgcacacgc ccgtgttggg gacgatctcg tcgagcgggc aaatcggcgt ccagacaagc
     4261 ggaaggcggt caaggttcat ggcgcaaggc ctcgatgaat gaggttaaaa aaagaatcaa
     4321 acggtttcga caaccacggg aatcgccgcg agcttcgatt gccgctcggt gagcgtcgcg
     4381 ggccggatct ggccgcgttc ctcgacgaat ttgacgttgc tgtccgactc gccgctgttg
     4441 acgaagtgac gaaagcgctt gcgcgtttca ggatcggtga cggccttttt ccattcgcat
     4501 tcataggtct cgaccacgtg ctgcatttcc ccctcgagct cggcggccac gccaaggcga
     4561 tcgttgacga tcacgtcgat cagatagtcg agtccgcctt cgaggttgtc gcgccacacg
     4621 ctggtgcgtt gcagacggtc cgcggtgcgc acgtagaaca tcaggaaacg gtcgatgtaa
     4681 cgcaccagcg tgtccttatc gaggtccgag gcgagcagtt cggcatggcg cggcttcatg
     4741 ccgccattgc cgcacacgta gagattccag cctttctccg tcgcgatgat gccgacgtcc
     4801 ttgccctgcg cttccgcgca ttcgcgcgcg cagccggaga cgccgaactt gatcttgtgc
     4861 ggcgtgcgca ggcccttgta gcggttctcc aagtcgaccg cgaggcccac cgaatcgccg
     4921 acgccatagc ggcaccacgt cgagccgaca cacgacttca ccgtgcgcag cgatttgccg
     4981 tatgcatgac ccgattcgaa accggcggcg atcagttctt cccagatgag cggcagttgc
     5041 tcgacgcgcg cgccgaacag atcgacccgc tggccgccgg tgatcttcgt atagaggccg
     5101 tatttcttcg cgacctggcc gaccgcgatc agaccgtcag gcgtcacttc tccccctgcc
     5161 attcgcggca cgaccgaata ggtgccgtcg cgctggatgt tggcgaggta gtagtcgttg
     5221 gtgtcctgca gcgaggcgtg ctctttcttc agcacgaatt cattccagca cgacgcgagg
     5281 atgctcgcca cgactggctt gcagatatcg cagccgagac cgtgtccgtg cttcgccagc
     5341 aattcgccga agctgcgcac gccctccacg cgcacgaggt gatacagctc ctggcgcgaa
     5401 taggggaagt gctcgcagac gtggttgttg acggccatgc cctgcttctt catttcggcc
     5461 ttcatcacct gggtgacgag cgggacgcag ccgccgcacg aggtgcccgc gctggtcgcg
     5521 cttttcaacg cgccgatgtc ggtcgcgccc gcgcggacgg cggcgcatag gtcgcccttc
     5581 gagacgttgt tgcacgagca gatttgcgcg ccggcaggca atgcctcgac gccgagcgcc
     5641 ggtttcgcct gaccgtcgga cgacggcagg atcaggaact cgggcgaggc cggcaactcg
     5701 atgcggttga gcatcatctg cagcagcgtg ccgtattcgc tcgcgtcgcc gaccatcacg
     5761 ccgcctagca gatatttgcc gcactcggac accaccagct tcttgtagac ctgcttgcgc
     5821 tcgtcgctga actgataggc gcggctgccc ggcgcattgc catgcgcgtc gccgatgctc
     5881 gccacatcca cgcccatcag cttcaatttg gtgctcatat cggcgcctgc gaattccgcc
     5941 gggtcgccga gcagttgttt cgccaccgcg cgcgccatgt cataacccgg cgcgacaagg
     6001 ccgaacagtt gaccgttcca caaggcgcat tcgccgatcg catagatgtg cggatcgctg
     6061 gtgcggcatg cgttgtcgat cacgatgccg ccgcgtgcgc cgagcgtcag gccgctttcg
     6121 cgcgcgaggt cgtcgcgcgg acgaatcccc gccgagaaca cgatcatgtc ggtatcgagg
     6181 aagctgccgt ccgcgaattg catgcgatgc gtgccgttct ccccgtcgac gatcgctgtc
     6241 gtgttcttct gcgtatgcac cgtcacgccg agttcttcga tcttgctgcg cagcacgcgg
     6301 ccgccgccgt catcgacttg cacggccatc aggcgcggcg cgaattcaac cacgtgcgtt
     6361 tgcaggccca tgtcgcgcaa cgccttcgcg cattcgagcc cgagcaaacc gccgccgacc
     6421 accgtgccgg tcttcgagcg agtaccgcat tcctgcatgg cttcgaggtc gtcgatggtg
     6481 cggtacacga aacagtcggc ccgttcgcgc cccggcacgg gcggcacgaa cggatacgag
     6541 ccggtcgcga acacgagctt gtcatagggc agcgtgtcgt acgtctggcc cgtcgatacc
     6601 gtcacggtgc gcgtatcgcg atcgatcgag acggccttcg cattcagcag gagccgcatg
     6661 ttctgccgct cgaagaagcc cgcttcgacc agcgacagat catctgccgt cttgcccgaa
     6721 aagaattcgg acagatgcac gcggtcatag gcgggccgtg attcttcgca cagcaccgtg
     6781 atgtcgaggc catgcgcggc gtcttgtgca aggcactcga ccagtttgtg gccgaccatc
     6841 ccgtgaccga taacgatgat tctcatgagc gcggcttcct cgatgtgctg ggctggaatc
     6901 gcctgcggct taggtgaggc ttaattgcgg ctcaactgcg ggctagctgc ggctttgccg
     6961 ctgccaggct gctgcgcgaa agcgattccg gaaataaaaa cggcgtcccg cgaacccagt
     7021 cgatgactga attcggggga cgccgttgtc ctgatctgtg ctgcagcggg cgctttgttg
     7081 ttgcgctcgc aacggatcaa cgttgatccg ctgagagggc ttaatgcaaa gcctgtgcca
     7141 atcaggcgaa atgtccgtta caacgccgtg gcgcggggcc tggcgacgaa ggcgaggagt
     7201 gggatgacag catcgcgcgc ggcacgtttt gatgcagcca ggcacaggga tggtgcgacg
     7261 cggcactgcg gccgtgcgag cagcgagcac gtgagacgcg ctatcggtgc actaacgatg
     7321 cgttactctg cctgcccacc aacgtggcaa cgcgcgcgcg acgatcgtca cgcgcttgag
     7381 taagcgaagc aaaaaatggt gcgaagacct ggcggcaatg caggtcaaag cgtgagtcgc
     7441 agccggcgcg aaacgcgcaa gccgcgcgaa ttcaaatccc gaccggcaat ctcgccacga
     7501 acaccgtgcc atcctcgaca gatgatctga cttccagcgc gccgccgtgc gcggagacaa
     7561 tctgcttgca gatatacagg ccgagcccca gcccaccacc cggcttgctg gtaggcggcc
     7621 gccagtaggg ctcgaacaca cggttcaacg cgtccggcgc aatcgcattg ccgccgttcg
     7681 acacggccag cacgaactcg ctgccctcca cgcgcgcttg cacgaccacc ggaaacgccg
     7741 ccgagccatg cgtcaccgcg ttgccaagca gattcgacag caactgctgg actcgcgtgc
     7801 ggtcgcagcg cacccgcgtg ccgatcgcga tatcgtcgac cagcatgcgc tccggattgg
     7861 cctcgcgcga ttcggcgatc accacccgca acgccattgc cagatcgtcg acgtcgtcga
     7921 tcgatacgcc gatgcccgag ccgagccggc cgcgcgcgaa atccatcacg tcgtcgatca
     7981 ggcgcgccat gcggaaggtg gtggtcttca ggcgctgacc gatcttcacg agatcaggct
     8041 cgttcttgcg cagcgccagc agttcggcgg ttgcgctgac tgcggatagc gggttgcgca
     8101 gatcgtggcc gagcaccgcg atgaactgtt cgcgcaggct ggaaatttcc cgttcgcgcg
     8161 ataacgcctc ttcggccgcc tgttgcctgc cctcggtcgc gatctggagc gcgatcagct
     8221 cggcaaacac ctcgaacatg ctcagcgtgc gtgcgtccga aatctcgttc gggttcggat
     8281 cgatcgcgca gagattaccg aaatagctgc catcgggcaa aacgatcgga acggaaatat
     8341 aactgcccag attgtagatc cgcgctgtgt gatgaccttt atagacaggg tcgttgccaa
     8401 aattgtcgat cacgatcgac gcgcgcgcgg cgcgcgactc gaagcacaac gtcgtgtgca
     8461 gatcgagctg tccaccggcg gctaaaccga agttcacttc gtcctgcacc gcgcaggccg
     8521 tccagctttg ctccgtgacc cgggcgacgg cggcaaagcc catcccggtg ttcttgcaga
     8581 tcaggcgcag gatcgacggc acggcatcga tgcggcggat cgtctcgatg tcccgtgtga
     8641 tttgtgccgc ctcatcgtct ccaagcggta atttattcat aagaaggggt gccagatacc
     8701 ttgccggaac ggccaaaaaa caaaatattg agcctcgatt ctatcgggac ttcgaattgc
     8761 tcaatacaag gaacgcaacc tggagaaacc ggatgtccac tgacgatgcc gcgcgcctgc
     8821 tgatcgccga cgacgatccg aatctgctgg ccgcctacgt actcttcttc agcgcgcatg
     8881 gtttcgacgt acggaccgcg cgcaacggcc ttgacgcgct cgcgcagtat tgcgcgtggc
     8941 acccgcatgc ggcgctgctc gacgtcgaaa tgccgcgtct cgacggacgc gccgtcgcgc
     9001 ggcgcatccg ctacgtggcc gatacgccgg cgcccatgct ggtcgccgtg accggcctcg
     9061 cgtgcgacga ggacaggaac gagtcgttac gctccggatt caaccatcat ttcgtcaagc
     9121 ccgtgccgat gccggtgatc ctcgcggcac tgacaggacg tgcctggcat tgacgccgcg
     9181 agcgacgtac cttgcatggg gtgcctcctc ccgaggcgcc gtgtgcgata cggtccttct
     9241 gcgcgctgat tctgtcgttc agctcaacgc cagccgcaca tccccgtcta gtcgccaaaa
     9301 cccaattccg cttccactgc ttgcccgact cgcagccgcg ccccggcgcc gcgcgcgacg
     9361 atcgcattgt tgccgaacgt cagcgcaccg tttcgccgcg gacttgcccg gtagacactc
     9421 atggtgtcgg tgggttcatt cggccaatcc ggatcgggcg cgcccttcgc ctggtcgatg
     9481 gtcggcatcg gacagcgcga gcacagcttg accagttgca gctggacggc cgcgccctca
     9541 gcgccgtcga cactcaagct ctcgacataa tcctcttcgt acgcatcgag tcccgttagt
     9601 acgacgttgg ggcgaaaccg gtcgatcgga atcgacggcg cgcccttgtt gttcagccgc
     9661 acattcagat cgtcgagcga ggcctggccg accaccagca gcggaaaacc gtcggcgaaa
     9721 tgcgtggtgg cgccgccgac gctgccggtg tagtccggat cgacgatccg ctcgcgctcc
     9781 ggatcgaagc gcagcagtcg cgccggcacg ccgagaaagt ccgaaaacca cgcggcgctt
     9841 tgctcaccgg tgtcgagccc gtaggccgca tcgcgccaga ccgcggtttg caccttccgc
     9901 gcggcgccaa gcccggccgc ttcaagcggt gtgcgcaacg cgctcatgcc cggcgcgcgg
     9961 atcacgagtt catgttcgcc gagctcgacc ttgaccagtg ccatgcgggg atacgcgcgc
    10021 tgcgtgagca tcgcgccgct cggctcggtg accatccagc atcgatcgta ttcgaggcca
    10081 gtggcgagca gacgcgcttc gctgagcgca atgcctgcgc aggatttgat cggatagacg
    10141 aaaagctcgc tgatggtagg catgatgtgg agcgtgggga acacgcgttt ttcttgtgtt
    10201 gatgctcgat tgtaaagctt gtaaagcgcc ttgcgacgcg ccagattgac cgcgccatgg
    10261 cgccggtttc cattgcctgt tgaacgctgc accgcctatg ccgaaccttg cagctgaact
    10321 gctgcgccct attcccagcc ttcgagccgc agcgtcgcgc gcacgagccg ctgctcttcc
    10381 tgttcgattt cgcgcaggtt cgcacatacg ctttcgatgt gatcggccgc cgccttgcgc
    10441 gcctgttcgg gcagcttgcc ggtgaccgcg ttgtagagcc gtgcatgctg acgatcgatc
    10501 acccgctttt gcggctcgcg gtgatagaga ttgttcaccg aagcaaacac cgaactcaac
    10561 agcagatcgg tcagcgtggt ccaggtatgc accagcaccg ggttgtgcga agcctcgcaa
    10621 atcgcaaggt gaaacgcgtg atcgaggcgc gcgtgggtag acgcgctcgt ctctgtgccg
    10681 tgcgccgcga gcatttcctc gtagcggcgc gtgatcagca cgaagtcggc ggccgtgccg
    10741 cgcagcgccg caagccgggc ggattccgct tcgagcaagg cgcgcacttc aaacagatcg
    10801 tagagcgtgc gcggctgcga gccgagcaga tgcatcatcg gtgtgagcgt gggttgcgcg
    10861 gtgaggctgg cgacgaacga gcccctgcca tgcgaggttt cgatgatgcc gcgcgcccgc
    10921 aacagcttga gcccttcgcg caaggccgtg cgcgaagggc tcaagctgtt gc
//
LOCUS       DATASH010000238         5151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_624117,
            whole genome shotgun sequence.
ACCESSION   DATASH010000238 DATASH010000000
VERSION     DATASH010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5151
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624117"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..932)
                     /locus_tag="VHV99_06670"
     CDS             complement(219..932)
                     /locus_tag="VHV99_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HEX3635161.1"
                     /translation="MSPYIAEFIGTALVVLLGDGAVANVLLARTKGKGADLIVIVMGW
                     AMAVFIAVYVTASFSGAHLNPVVTISLALAGKFAWAKVPGYVAAQMLGGMAGALLVWI
                     AYRQHFNKEGDADVKLGVFCTSPAIRSVPHNLLTEMIATFVLILGVLYLASPQVGLGA
                     LDALPVGLLVLGIGISLGGPTGYAMSPARDLSPRLMHALLPIPGKRDSDWRYSWIPVC
                     GPLLGGAVAAALYLYLHAH"
     gene            complement(1018..2517)
                     /gene="glpK"
                     /locus_tag="VHV99_06675"
     CDS             complement(1018..2517)
                     /gene="glpK"
                     /locus_tag="VHV99_06675"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEX3635162.1"
                     /translation="MQDQYILALDQGTTSSRAMLFDRLGNIVSSAQKEFQQIYPHPGW
                     VEHDPQEIWSTQAGVAAEAVTRASMNGTSIAAIGITNQRETTIVWDRETGHPIYNAIV
                     WQDRRTADFCDQLKEQGLEEKVRVKTGLPIDSYFSATKIRWILDNVEGAREKAKQGRL
                     AFGTVDSWLVWNFTKGGLHVTDVTNASRTMLFNIHTLKWDDELLEALDIPRNMLPEVR
                     ASSEVYGPTKTTVFASRIPLAGIAGDQHAALFGQMCTESGMVKNTYGTGCFLVMNTGD
                     KPIESKNNLVTTIAWQIGDQINYALEGSIFIGGAVVQWLRDGLGLIKNAAEIETLARS
                     VPHCDGVYLVPAFAGLGAPHWNARARGTLFGVTRGTSSAHIARAALDSIAYQSLDVLK
                     AMEADSGIRIGELRVDGGACANNLLMQFQADILGVDAVRPKVSETTALGAAYLAGLAV
                     GYWKDVNELKSQWALDRRFTPALPHADVKECLDGWKRAIRAAKAWADTP"
     gene            complement(2685..4214)
                     /gene="glpD"
                     /locus_tag="VHV99_06680"
     CDS             complement(2685..4214)
                     /gene="glpD"
                     /locus_tag="VHV99_06680"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HEX3635163.1"
                     /translation="MTQGSRYDVLVVGGGINGAGIARDAAGRGLSVLLCEQDDLAAHT
                     SSASTKLIHGGLRYLEYREFGLVRKALQERETLLRAAPHIMWPLRFVMPHMPDLRPAW
                     LIRAGLFLYDHLAKRELLPGSRGIVMRNHPAGVPLVDSIKRGFVYSDGWVNDARLVLL
                     NALDAQERGAKILTRTKLLSAVRAGGEWRAQLKRADGTILDVRAGSIANAGGPWVGEL
                     LQGALGREATHSVRLVKGSHIVTRRLFEHDHAYIFQNPDKRIIFAIPYEHDYTLIGTT
                     DLEYRGDPSQVAISAGETQYLCDSINRYFKQKISPEDVRWTYSGVRPLLEEEGADNPS
                     AVTRDYSLELDAPVGEAPLLSVFGGKITTFRKLAEEAVDQLAHALYNGAPSWTAGVPL
                     PGGDIPQANFERFLAGFMQQNAWLPADLAHRLARAYGTRVKHVIGNARSVADLGRVFA
                     PGLYEAELTYLRDAEWARSAQDVLWRRSKLGLHVEPGTLDSITHNIDTWFAREPVRQS
                     A"
     gene            4424..4798
                     /locus_tag="VHV99_06685"
     CDS             4424..4798
                     /locus_tag="VHV99_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635164.1"
                     /translation="MRGLWIIGAAALLTGCVSSPSLNGTMGAPSFASLQQMCSAQPVD
                     YGNDAQGVYATLFDAYVANRRGKLSKDDYCAFQASLAQRYASQGVSTDPQVRNQWVAF
                     FIDQRAKALSWRAAVDPTLRAG"
     gene            complement(4878..>5151)
                     /locus_tag="VHV99_06690"
     CDS             complement(4878..>5151)
                     /locus_tag="VHV99_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="HEX3635165.1"
                     /translation="PELTAASQRAWQLLGVHFEQLVAAGILEGDPQLIGYAYWASLHG
                     FTMLTLANQLPLSQALQAAASNAPTREAVFAQIRRMLWRGAQPQRG"
BASE COUNT          912 a         1576 c         1684 g          979 t
ORIGIN      
        1 aagcgagagg cacgcgagca ggacagttgc aagctcatgc gcgaagattg aaagcgtcat
       61 gatacgtgct ttggcgccgc atgcgcggcg ccaaagcttt ccgtcggcgc tagttgcgcc
      121 tcgagcgcgc gtttagtgtg catcgtgtgg gcgcttggat gcgccttaag tgccccctaa
      181 atggtgcgct ctaagtgcgt cataaagtca ccgcggaatc agtgcgcgtg caggtacaga
      241 tacagcgccg cagccaccgc accgcccagc agcgggccgc acactggaat ccatgaatag
      301 cgccagtcgc tgtcgcgctt gcccggaatc ggcagcaacg cgtgcatcag acgcggcgag
      361 aggtcgcgcg ccgggctcat cgcgtaaccg gtcgggccgc caagcgaaat accgatgccg
      421 agcacgagca ggccgaccgg cagcgcgtcg agcgcaccta ggccgacttg cggcgaagcc
      481 agatacagca cgccgagaat cagcacgaag gtcgcgatca tttcggtgag caggttatgc
      541 ggcacgctgc gaatcgcggg cgatgtgcag aacacgccga gcttcacgtc agcatcgcct
      601 tccttgttga agtgctgacg atacgcgatc cacacgagca gggcgcccgc catcccgccg
      661 agcatctgcg cggcgacgta gccaggcact ttggcccacg cgaatttgcc cgccagcgcg
      721 aggctgatcg tcacgacagg attcagatgc gcgccgctga acgatgcggt gacgtacacg
      781 gcgatgaaca cggccatcgc ccaacccatc acgatcacga tcaggtccgc gcctttgccc
      841 ttggtgcgcg cgagcagcac gttggcgacc gcgccgtcgc cgagcagcac cacgagtgct
      901 gtgccgatga attccgcaat gtaaggagac atcgttggtt atccgaaata tagttgttac
      961 ggaagccgcg ggataagcgg cttcgttatg tcgaaggaga ttgcgcgaga gcggcaatca
     1021 gggcgtgtcg gcccaggcct tcgcggcgcg gatcgcgcgc ttccagccgt cgaggcattc
     1081 cttgacgtcg gcgtgcggca gggcaggcgt gaaacggcga tcgagcgccc actggctttt
     1141 cagttcgttc acgtctttcc agtagccgac cgcgagcccg gccagatacg ccgcgcccaa
     1201 ggcggtcgtc tccgagacct ttggacgcac tgcatcgacg ccgagaatgt ccgcctggaa
     1261 ttgcatcaac agattgttcg cgcacgcgcc gccgtccacg cgcaattcgc cgatgcgaat
     1321 gccggaatcg gcttccatcg ctttgagcac gtcgagcgat tggtaggcga tcgagtcgag
     1381 cgccgcgcgg gcgatgtgcg ccgagctcgt gccgcgcgtc acgccgaaca gcgtgccgcg
     1441 cgcgcgggca ttccagtgcg gcgcgcccag gccagcgaag gcgggcacca gatatacgcc
     1501 gtcgcaatgc ggtacgctgc gcgccagcgt ttcgatttca gcggcgttct tgatgagccc
     1561 gaggccgtcg cgcagccatt gcaccaccgc gccgccaatg aagatgctgc cttcgagtgc
     1621 gtagttgatc tgatcgccta tctgccaggc gatcgtggtg accagattgt tcttcgattc
     1681 gatcggcttg tcgccggtgt tcatcacgag gaagcagccg gtgccgtagg tgttcttcac
     1741 catgccggac tccgtgcaca tctggccgaa cagggccgcg tgctggtcgc cggcaatgcc
     1801 cgcgagcgga atcctggagg cgaacacggt ggtcttggtc ggcccgtaca cttccgacga
     1861 cgcacgcact tccggcagca tgttgcgcgg aatgtcgagc gcttcgagca attcgtcgtc
     1921 ccatttcagc gtgtggatgt tgaacagcat cgtgcgcgac gcgttggtca cgtcggtgac
     1981 gtgcaggccg cctttagtga aattccacac cagccagcta tccaccgtgc cgaaggcgag
     2041 gcggccttgc ttcgcttttt cgcgtgcgcc ttcgacattg tcgagaatcc agcggatctt
     2101 ggtggccgag aaatacgaat cgatcggcag acccgtcttc acgcggactt tctcttcgag
     2161 tccttgctct ttgagctgat cgcagaagtc ggccgtgcgg cggtcttgcc agacgatcgc
     2221 gttgtaaatg gggtggccgg tttcgcggtc ccacacaatg gtggtctcac gctgattggt
     2281 aatgccgatc gcggcgatcg aggtgccgtt catgctggcg cgggtgacgg cttcggccgc
     2341 aacgccggcc tgcgtcgacc agatttcctg cgggtcgtgc tcgacccaac ccggatgcgg
     2401 atagatttgc tggaattctt tctgagcgct cgacacgata ttgccgagcc ggtcgaacag
     2461 catcgcgcgc gagctcgtgg tgccctggtc aagcgcgagg atgtactgat cctgcattgt
     2521 ctcttctcca tgcgttttat gaatcgccgg actgctaacc ggcgacggga acggcttgta
     2581 gttttaagcg ggttgcaaca gtgtgagctt gtgcgtcccg gctttgtcag tggccgaggg
     2641 gtgaaaatcg attcggttca tgcgggttcg tgcagcggcg ccggctaagc gctctgccgg
     2701 accggctcgc gggcgaacca ggtgtcgatg ttatgcgtga tcgaatcgag cgtgcccggt
     2761 tcgacgtgca ggccgagttt cgagcggcgc cacaacacgt cctgtgcgct gcgcgcccat
     2821 tcagcgtcgc gcaaataagt cagttccgct tcgtagagac ccggcgcgaa cacgcggccg
     2881 aggtcggcga ccgagcgcgc gttgccaatg acgtgcttca cgcgcgtgcc gtatgcacgg
     2941 gcgaggcggt gggccagatc cgcgggcagc cacgcgttct gctgcatgaa gccggcgagg
     3001 aagcgctcga agttggcttg cggaatatcg ccaccgggca acggcacacc cgccgtccac
     3061 gaaggcgcgc cgttatacag cgcgtgcgcg agttggtcga cggcttcttc cgccagcttg
     3121 cggaaggtcg tgatcttgcc gccgaacacc gacagcagcg gcgcttcgcc cacgggcgcg
     3181 tcgagttcga gcgagtaatc gcgcgtgacc gccgaggggt tgtccgcgcc ttcttcctcg
     3241 agcagcggac gcacgcccga ataggtccag cgcacgtctt ctggcgagat cttctgcttg
     3301 aagtagcggt tgatcgagtc gcacaggtac tgggtttcgc cggcgctgat ggcgacctgc
     3361 gaagggtcgc cgcgatattc gaggtcggtc gtgccgatca gcgtgtaatc gtgctcgtag
     3421 ggaatcgcga agatgatccg cttgtccgga ttctgaaaga tgtacgcatg atcgtgttcg
     3481 aacagacggc gcgtgacgat atgactgcct ttcacgaggc gcacgctgtg ggtggcctcg
     3541 cggccgagcg cgccttgcag caattcgccg acccacgggc cgcccgcatt cgcaatcgag
     3601 cccgcgcgca cgtcgaggat tgtgccgtcc gcgcgtttaa gttgcgcgcg ccattcgccg
     3661 ccggcgcgca ccgcgctcag cagtttggtg cgcgtgagaa tcttcgcgcc gcgttcctga
     3721 gcgtccagcg cgttcagcaa gaccagacgc gcgtcgttca cccaaccgtc cgaatacacg
     3781 aaaccacgtt tgatcgaatc gacgagcggt acgccggcag gatggttgcg catcacgatg
     3841 ccgcgcgagc cgggcagcaa ctcgcgtttg gcgaggtgat cgtaaaggaa cagaccggcg
     3901 cggatcagcc aggccgggcg tagatcgggc atgtgcggca tcacgaagcg cagcggccac
     3961 atgatgtgcg gcgccgcgcg cagcagtgtt tcgcgctcct gcagggcttt gcgcactagc
     4021 ccgaattcgc ggtattcgag gtagcgcaga ccgccgtgaa tcagcttggt gctagccgac
     4081 gacgtatgcg ccgccagatc atcctgttcg cagagcagca ccgacaggcc gcggcccgcc
     4141 gcgtcgcgtg cgatgcctgc gccgttgatc ccgccgccca cgacgagcac atcgtatctc
     4201 gagccttgcg tcacctgctg tgtcccctgc gccgtgtccg gaaattcgga aaaccgtttg
     4261 gaaaatctat cgaaccgata gtgttcgttt tcgaacttta atgaacatat tcgaaaaagt
     4321 aaagttcgat tgcctgatgc gccctctatc gattggctaa ggtggtggcg taacgggcgg
     4381 ccacggacga tactctcggc agcagccatt tcgaggtgaa ttcatgcgtg gactttggat
     4441 aatcggcgcc gctgcgctcc tgacgggatg cgtcagctcg ccgagcttga atgggacgat
     4501 gggcgcgccc tcgttcgcat cgttgcagca gatgtgcagc gcccagccgg tcgactacgg
     4561 taatgacgcg caaggcgtct acgcgacgct gttcgatgcg tacgtggcga accggcgcgg
     4621 gaagctgtcg aaggacgatt actgcgcgtt ccaggcgtcg cttgcgcagc gctatgcgag
     4681 tcaaggggtg agtaccgatc cgcaggtgcg taatcagtgg gtggcgtttt tcatcgacca
     4741 gcgagccaag gcgctgagct ggcgtgcggc ggtcgatcct actttgcgcg cgggctgagc
     4801 ttcgcgcttt gtttccgttt ggccggtact tcccggcggg caagccgtgc agggtgggtg
     4861 gatgggccaa cccggcgcta accgcgctgt ggctgggccc cgcgccataa catccggcga
     4921 atttgcgcga acaccgcctc gcgcgttggc gcgttgctcg cggcggcttg cagcgcctgg
     4981 gacaacggca gctgattggc cagcgtcagc atggtgaagc cgtgcaggct tgcccagtac
     5041 gcatagccga tcaattgcgg atcgccttcc agaatgcccg cggcaacgag ctgctcgaag
     5101 tgcaccccga gcagttgcca cgcgcgctgc gacgcggccg tgagttccgg g
//
LOCUS       DATASH010000239         3636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1219173,
            whole genome shotgun sequence.
ACCESSION   DATASH010000239 DATASH010000000
VERSION     DATASH010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219173"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VHV99_06695"
     CDS             <1..307
                     /locus_tag="VHV99_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEX3635166.1"
                     /translation="TFYTGRLRERYGLETLLPDETERADVHRIIYDELCHGNVNDTSR
                     AVYQHVIEGLAARGAQAVILGCTEITLLIKPENSALPVFDTTALHAQAAVQWAIDSD"
     gene            complement(430..506)
                     /locus_tag="VHV99_06700"
     tRNA            complement(430..506)
                     /locus_tag="VHV99_06700"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(470..472),aa:Met,seq:cat)
     gene            complement(620..907)
                     /locus_tag="VHV99_06705"
     CDS             complement(620..907)
                     /locus_tag="VHV99_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635167.1"
                     /translation="MAQLYPALYKDFEVHPLVFSRQFDKFDGHKRHAEGYDVAVRVCR
                     PGAISGSKASRVFRLVLTSTFSDFGVAKRSASQYGADIIDGKVDGATVEDL"
     gene            1157..1933
                     /gene="xth"
                     /locus_tag="VHV99_06710"
     CDS             1157..1933
                     /gene="xth"
                     /locus_tag="VHV99_06710"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX3635168.1"
                     /translation="MKIATWNVNSLKVREQHVIDWLGTSQTDVLCLQELKLTDDKFPR
                     AALEEKGYQSWFAGQKTYNGVGILVRDGLNVDPSTIVRNIPGFEDPQQRVIAATVDGV
                     RIISAYFPNGQAPGTDKFAYKLRWLAALHDWIAREMALYPKLALLGDYNIAPEDRDVH
                     DPKAWEGQNLVSPEERAAFVQLIGLGLLDAFRQFEQPEKIYSWWDYRMMAFRRNAGLR
                     IDHILLSKALADVCSSCDIDKVPRKWDQPSDHAPVVAQIG"
     gene            complement(1951..>3636)
                     /locus_tag="VHV99_06715"
     CDS             complement(1951..>3636)
                     /locus_tag="VHV99_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX3635169.1"
                     /translation="VIAKAGKHTASWIDRDTLYVGWDNGRKTLTRSGYPREVRRWTRG
                     SALAEAPVVFKGAFGDIGVEAHYDPVERRHTVVSSVDFFDSQTYYLDGADVDAGDAGN
                     RATAASNGSIANNANNASAWRQYDVPSYVAVGGWQGWLLLEPRLDWDCNGVRYPGGAL
                     LAIREAALLRGERDVVPLFTPTSQTSACEWTHTRNHLIVSYLEDVRSKTSLWTPSQAA
                     DQAWHWHQRVFPSRDDEEADVSPVEPTLNDEVFVDTDDYLKPPAYWLTDLAQDDLADW
                     ALLDRWPTQFDATPFVVTRAHAVSADGTRVPYTVIGPRDAQHPVEGLQTHNQTRPCLL
                     NGYGGFAIPLLPSYLTGQGIGWLERGGVYVVAHIRGGGEFGTEWHTAAQGEHRQRAFD
                     DFIAVAEALISTGVTSAAQLGIQGGSNGGLLVAACMVQRPELFGAVVCEVPLLDMRRY
                     HLLHAGASWIDEYGDPDEPDEARVLAAYSPYHRVSADVAYPPVLFTTSTADDRVHPGH
                     ARKMAARMQALGAQSVWYRENTEGGHGGSDELEQAEHDAMVFEFLWRCLNGAA"
BASE COUNT          744 a         1168 c         1076 g          648 t
ORIGIN      
        1 gactttctac acgggccgct tgcgcgagcg ctacggcctc gagacactgc tccccgatga
       61 aaccgaacgc gcggatgtgc accgcatcat ctacgacgaa ctgtgccacg gcaacgtgaa
      121 cgacacttcg cgtgcggtct atcaacatgt aatcgaagga ctcgccgcac gtggcgcgca
      181 agcggtgatt ctcggctgca cggaaatcac cttgctgatc aagccggaaa actcggcgtt
      241 acccgtgttc gacacgaccg cgttgcatgc gcaggcggca gtgcaatggg cgatcgattc
      301 ggactgaatg caagcgtgcg tggcggctgc gtggcgcggc ccgattgctt cgtccgggcg
      361 cgcatgataa gagagaggcc agaagtcaaa aagcccgcta gcttgcgcta gcgggctttc
      421 aacaatgctt ggttgcgggg gtaggatttg aacctacgac cttcgggtta tgagcccgac
      481 gagctgccag actgctccac cccgcgtcag agaaaaaaat tatagcgggt tgtcagcaag
      541 acgtcaaaca gttcaatgaa atactttcaa acgaggtacg cagcacgtgt cctcgcccct
      601 gcagcgatga gcaagcaact caaagatcct cgaccgtcgc gccgtccacc ttgccgtcga
      661 tgatgtccgc gccgtactgg cttgcgctgc gctttgcgac gccgaaatcg gagaacgtgg
      721 acgtgagcac gagacgaaat acgcggcttg ccttcgagcc gctgatcgcg cccggccggc
      781 agacgcgtac cgcaacgtcg taaccttcgg catgacgctt gtggccgtcg aacttgtcga
      841 actgccgtga aaacaccagc ggatgcactt cgaagtcctt ataaagggct ggataaagtt
      901 gagccatgga ccgtccccga tgcgcgtaaa tgtgatccca ctatacgccg cggcatgaca
      961 gcgtcgggct tttatttttc acggcgggcg cggcgatttt cactgtcgcg cgcctcgcgc
     1021 gataagatgc ggggctatcg gcatgagccc gatcctgcca acccgggaac tgggtcgtca
     1081 gacgggcaga taaagcaata aagcaaaaac gaggggcaac gcccttcaaa gactttgcac
     1141 agaatgtgag aacttcatga aaatcgccac ctggaacgtc aactccctga aagtacgcga
     1201 gcagcatgtg atcgactggc tcggcaccag ccagaccgac gtgctgtgtc tgcaggaatt
     1261 gaagctgacc gacgacaaat tcccccgcgc cgcactcgaa gagaaaggct accagagctg
     1321 gttcgcgggc cagaaaactt acaacggcgt cggcattctg gtgcgtgacg gcctgaacgt
     1381 cgacccgagc accatcgtgc gcaatatccc cggcttcgag gatccgcagc aacgcgtgat
     1441 tgccgcgacg gtcgacggcg tgcgcatcat ctccgcgtac ttcccgaacg gccaggcgcc
     1501 gggcaccgac aagttcgcct acaaactgcg ctggcttgct gcgctgcacg actggatcgc
     1561 gcgcgaaatg gcgctctacc cgaagctcgc actgctcggc gactacaaca tcgcaccgga
     1621 agatcgcgac gtacacgatc cgaaagcatg ggaaggccag aatctggtgt cgcccgaaga
     1681 gcgcgccgcg ttcgtgcagc tgatcggact cgggctgctc gatgcattcc gccagttcga
     1741 gcagccggaa aaaatctatt cgtggtggga ctaccggatg atggccttcc gccgcaatgc
     1801 ggggctgcgc atcgatcaca ttctgctgtc gaaggcgctt gccgacgtgt gctcgtcttg
     1861 cgatatcgac aaggtcccgc gcaaatggga tcaaccgtcg gaccatgcgc cagttgtcgc
     1921 gcagatcggc tgaacgcgtg acgaaagagg ctacgccgcc ccgttcaagc agcgccacaa
     1981 gaattcaaac accatcgcat catgctcggc ctgctccagt tcatcggagc cgccgtggcc
     2041 tccttccgtg ttctcccgat accagacact ttgcgcgccc agtgcctgca tgcgcgcggc
     2101 catcttgcgc gcgtgtccgg ggtgcacgcg atcgtcggcc gtcgatgtgg tgaacagcac
     2161 gggcggatac gccacgtcag cactcacacg gtgatagggc gagtaggcag ccagcacacg
     2221 cgcttcgtcg ggctcgtccg gatcgccgta ttcatcgatc catgacgcac ccgcgtgcag
     2281 caggtgataa cgacgcatgt cgagcagcgg cacctcgcag accacggcgc cgaacaactc
     2341 aggccgctgc accatgcacg ccgcgaccag caggccgcca ttgctgccgc cctgaatgcc
     2401 gagctgtgcg gcactggtca cgcctgtgct gatgagcgcc tcggccaccg cgatgaaatc
     2461 gtcgaacgcg cgctgccgat gctcaccctg cgccgccgtg tgccactctg tgccgaactc
     2521 gccgccgccg cggatatgcg ccaccacata tacaccgccc cgttccagcc agccgatgcc
     2581 ctgcccggtc aggtagctcg gcagcaaggg aatcgcgaaa ccgccgtatc cgttgagcag
     2641 gcatgggcgg gtttggttgt gcgtttgcag cccttctacg gggtgttgcg catcccgtgg
     2701 tccgattacg gtgtacggca cccgcgtgcc gtccgcggac accgcgtgcg cacgcgtcac
     2761 gacaaacggc gtcgcgtcga actgagtcgg ccagcggtcg agtaacgccc aatcggccag
     2821 gtcatcctga gccagatcgg ttagccagta tgcaggcggc ttcaaataat cgtcggtatc
     2881 gacgaacact tcgtcgttca gcgtcggctc gacgggcgat acgtcggctt cttcgtcatc
     2941 acgcgacgga aagacgcgtt gatgccaatg ccacgcttga tcggccgctt gcgacggagt
     3001 ccacagcgaa gtcttgctgc gcacatcttc cagatacgac acgatcagat gattgcgcgt
     3061 gtgggtccac tcgcaggcgg aggtttgcga tgtcggcgtg aagagcggca ccacgtcacg
     3121 ctcaccacgc aacaatgcag cttcgcggat cgccagcaac gcgccgcccg gatagcgcac
     3181 accgttgcaa tcccagtcga ggcgcggctc gagcagcagc caaccctgcc atccgccgac
     3241 cgcgacgtat gacggtacgt cgtactgtcg ccaggcactg gcgttgttag cgttgttagc
     3301 gatgctgccg ttgctggcag cagtcgcgcg attgcccgcg tcgcccgcat caacatcggc
     3361 accatcaagg taatacgttt gcgaatcgaa gaaatcgacg ctgctcacca ccgtgtgacg
     3421 ccgctcgacc ggatcgtagt gcgcctccac accgatatcg ccgaacgcgc ccttgaagac
     3481 caccggcgcc tcggccagcg cactgccgcg cgtccagcgc cgcacttcac gcggatagcc
     3541 ggagcgcgtc agcgtcttgc ggccgttgtc ccagccgaca taaagcgtat cccgatcgat
     3601 ccacgaagcc gtgtgcttgc ctgccttcgc aatcac
//
LOCUS       DATASH010000240         9448 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5373335,
            whole genome shotgun sequence.
ACCESSION   DATASH010000240 DATASH010000000
VERSION     DATASH010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 9448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9448
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5373335"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VHV99_06720"
     CDS             <1..445
                     /locus_tag="VHV99_06720"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX3635170.1"
                     /translation="TPGDALHVVQVGAWRIGVLVCYDRRFPECWRALRAAGADLVIVP
                     VAGSGGDDMDFFVGELRTHARENGLAVVCANKIGDEFLDGVRIDNYGESCVITADGAV
                     LGRRPGNEGPGIVSATLAHAEIAATREKLSYFDHRRVDLYGTPSF"
     gene            478..2019
                     /locus_tag="VHV99_06725"
     CDS             478..2019
                     /locus_tag="VHV99_06725"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3635171.1"
                     /translation="MSETLIVAGWIVKGVADRHTAEVIRDGALYQRDGVIVEIGDAQQ
                     LIAKYPNAPRLGSPDTVLLPGFVNSHHHVGLTPLQHGSPDYALELWFASRMGTREIDL
                     YLDTLYSAFEMIASGITTVQHIHGWRPGPLSSIHDAASGVLKAYRDIGMRASYSFAVR
                     EQNLLVYEADEDFVKRLPADIGAKLAAHLKAQQVPLADHLQLFDMLTQDNEGQSLTRI
                     QLAPANLHWCSDETLLAIKERADKAGVPMHMHLLETAYQKEYARRRTGKTAVAHLNDL
                     GLLGPSLTLGHGVWLTEADIEMVAHTGTCICHNCSSNLRLRSGVAPLNAFAKYGVTVG
                     MGLDEAGINEDRDMLQEMRLALRVHRTPGMDDDVPTCPQILKMATEDGARTTPFGAQI
                     GRLETGRLADLVLMNWKTATYPYQDDNIPMLDALMQRAKTNAVDAVMIAGDVVYRDGQ
                     FTRVDRDAILNEIEQTLAKPRNERKQAERELGAAVFPYVKAFYSDYFGETPPRQPFYA
                     PSSRN"
     gene            3172..4128
                     /locus_tag="VHV99_06730"
     CDS             3172..4128
                     /locus_tag="VHV99_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX3635172.1"
                     /translation="MTWQAPRQLWEVSQWDISMRQPIQPVSRVALILSPAVGDSLLMM
                     TIAHNLQKNCIAVTVFGQHTHALRDWFPAVDIRAELGEAHLAEKLHDFDTVIQMHSNR
                     PFAGLDRLHPRVITLDHIVSARSSESMADRLALFCRDELGLTNGEKSNGIMPPPGLNH
                     RKYPFRVAIHPTASTLDKRWLASRFIRLAMELRDRGFSPEFVVAPQERADWKHVERFG
                     ITLPDLSSLDHVATWLFECGWFIGNDSGIGHLASSLQIPTLSLFMRRGIARTWRPGWG
                     TGRVLIGGAYLPTGRLKERYWKYLLSVGKVTQAFEQLRTNLT"
     gene            4185..5075
                     /locus_tag="VHV99_06735"
     CDS             4185..5075
                     /locus_tag="VHV99_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007732502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX3635173.1"
                     /translation="MSNAIGDTLVLMVIVQNLLRNGFDVTAFGHPAYRLRHWFPNVAI
                     RPLPDNKDADALLARYDTVLQMHRDQPITRLTDVHPCVVYLDEIEFGDRSGCMAERFA
                     AFCRDELGLSNVDLYNGITPPPTLRHRHYIRRIAIHPEASTEDKRWLSGRFVKLAHNL
                     RQRGYDVNFVIAPHERGRWCELERWNIPAPEFSSPHELACWLYESGWFIGNDSGVGHL
                     ASNLSIPTISLFRRRRVSERWRPAWGTVNVVLPWQWVPTARLKEKLWRQTLTCARVLS
                     AFQSTVRDDRDTAKHDSSKR"
     gene            complement(5435..7018)
                     /gene="guaA"
                     /locus_tag="VHV99_06740"
     CDS             complement(5435..7018)
                     /gene="guaA"
                     /locus_tag="VHV99_06740"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006402676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEX3635174.1"
                     /translation="MHDKILILDFGSQVTQLIARRVREANVYSEIHPYDVDASFIRDF
                     APKGVILSGGPSSVTETDTPRVPQAVFELGVPVLGICYGMQAMAEQLGGKVDIGHLRE
                     FGYAEVRARNHTSLLEGISDFTTPEGHGMLKVWMSHGDKVLEMPEGFQLMASTESCPI
                     AAMADEKRHFYGLQWHPEVTHTVQGRAMLERFVLKICGARPDWEMGNYIDEAVAKIRE
                     QVGNEHVILGLSGGVDSSVAAALLHRAIGDQLTCVFVDHGLLRLNEAEQVMATFADHL
                     GVKVIHVDASEVFLSKLAGVTDPEAKRKIIGAEFVEVFQTEANKLTDAKWLAQGTIYP
                     DVIESAGKGKKAAQTIKSHHNVGGLPETLNLKLLEPLRELFKDEVRELGVKLGLPPAM
                     VYRHPFPGPGLGVRILGEVRRDFADLLRRADAIFIETLRTFIDKETGKSWYDLTSQAF
                     AVFLPVKSVGVMGDGRTYEYVVALRAVQTLDFMTAHWAHLPHELLGHVSNRIINEVRG
                     INRVVYDISGKPPATIEWE"
     gene            complement(7222..7950)
                     /locus_tag="VHV99_06745"
     CDS             complement(7222..7950)
                     /locus_tag="VHV99_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635175.1"
                     /translation="MKPLLRAVLILDAMLLLAFGLLFLLTPWTSLYNALLLVQTQPAL
                     VGQGFGVALIGLAWLALHASLDGSLTSTVAKVVGHVNWLTGVLMLVWLLGLHTPALTG
                     FGQIVAVLTGVVLLVIGLGGVRLSGAVRRRERARLVEAAAAERAEKQAAKDAAKDAAN
                     RRNAGRVTAGFPVYHAEPVVEPVMPVPRPLDPVAAAPLHPVVDEPGLTASERAARAEA
                     AAARDEARNSAADAPGAPRPPFHG"
     gene            complement(7957..9417)
                     /gene="guaB"
                     /locus_tag="VHV99_06750"
     CDS             complement(7957..9417)
                     /gene="guaB"
                     /locus_tag="VHV99_06750"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX3635176.1"
                     /translation="MRLIQTALTFDDVLLVPAFSDVLPRDTSLKTRLTRNISLNMPLV
                     SAAMDTVTEARLAIAMAQMGGVGIIHKNLTAAEQAREVAKVKRFESGVVRDPITVPPQ
                     MKVRDVIALSRQHGISGFPVVEGAQLIGIVTNRDLRFEERLDEPVRNIMTPRERLVTV
                     KEGTPLAQAKALMHSHRLERVLVINDAFELRGLMTVKDITKQTEHPDACKDEHGKLRA
                     GAAVGVGEDNEERVELLVQAGVDVIVVDTAHGHSKGVLERVKWVKRKFPHVEVIGGNI
                     ATAAAAKALVEYGADGVKVGIGPGSICTTRIVAGVGVPQVTAIANVSEALKGTGVPVV
                     GDGGVRFSGDVSKALAAGANSVMMGSMFAGTEEAPGDVFLFQGRQYKSYRGMGSVGAM
                     KDGAADRYFQDNSANIDKLVPEGIEGRVAYKGSVNAILFQLIGGVRASMGYCGCRTIA
                     EMNDKAEFVQITGAGLRESHVHDVQITKEAPNYHVD"
BASE COUNT         1857 a         2970 c         2753 g         1868 t
ORIGIN      
        1 cacgccgggc gacgcactgc acgttgtgca ggtaggggcg tggcgcatcg gcgttctggt
       61 ctgctacgac cgccgctttc cagaatgctg gcgcgcattg cgtgcggccg gcgcggatct
      121 cgtcatcgtg cccgtcgcgg gcagcggcgg cgacgatatg gatttcttcg tcggggagct
      181 gcgcacgcac gcgcgggaaa atgggctggc ggtcgtttgc gcgaacaaga tcggtgacga
      241 atttctcgat ggcgtgcgca tcgacaacta tggcgagtcg tgtgtgatta ccgccgacgg
      301 cgcggtactc ggccgccggc ccggcaacga aggacctggc atcgtcagcg cgacgctcgc
      361 gcatgcagag atcgcggcga cccgcgaaaa gctgtcttac ttcgatcatc ggcgtgtcga
      421 tctatacggc acaccatcgt tctgaaacta ctcttctcta ttcaggtaaa agcaaccatg
      481 tccgaaacat tgattgtcgc tggctggatc gtcaagggtg tcgccgaccg gcacaccgca
      541 gaggtcatcc gcgacggcgc gctgtatcaa cgcgacggcg tcatcgtcga gatcggcgat
      601 gcgcagcagt tgatcgcaaa gtatccgaac gcgccgcgtc tcggctcgcc cgacacggta
      661 ttgctgcctg gcttcgtcaa cagtcaccat cacgtcggcc tgacgccgct gcaacacggt
      721 tctcccgact acgcgctgga attgtggttt gcaagccgga tgggtacgcg cgagatcgat
      781 ctttacctcg ataccctgta ctccgccttc gaaatgattg cgtccggcat taccacggtg
      841 cagcatatcc acggttggcg gcctggtccg ctgtcgtcga tacatgacgc cgcgtcaggc
      901 gttctgaagg cgtatcggga catcggtatg cgcgcgtcgt acagcttcgc cgtgcgcgag
      961 cagaatctgc ttgtctacga agccgacgaa gacttcgtca aacgcctgcc ggccgacatc
     1021 ggggcgaaac tcgccgcgca tctgaaagcg cagcaggtcc cgctggccga ccatctgcag
     1081 ttgttcgaca tgctcacgca ggacaacgaa ggccaatcgc tgacccggat tcagcttgca
     1141 cctgccaacc tgcactggtg cagcgatgag acactgctcg ccatcaagga acgcgccgac
     1201 aaagccggcg taccgatgca tatgcatttg ctcgaaaccg cctatcagaa ggaatacgcg
     1261 cgccgccgga cgggcaagac ggcggtcgcg catctgaacg atctcggtct gctcggtccg
     1321 tcgctaaccc tcggccatgg tgtgtggctg acggaagcag acatcgaaat ggtcgcgcat
     1381 accggcacct gcatctgtca taactgcagt tcgaatctgc gtttgcgcag tggcgtggca
     1441 cccttgaatg cgtttgccaa atacggtgtg acggtgggca tggggctcga cgaagcgggt
     1501 atcaacgaag accgcgacat gttgcaggag atgcgcctcg cactgcgcgt gcaccgcacg
     1561 ccaggaatgg acgacgatgt gccgacctgc ccacagatac tgaaaatggc cactgaggat
     1621 ggcgctcgaa ccaccccgtt cggcgcacag atcggtcgcc tggaaaccgg acgcctggcc
     1681 gatctggtgt tgatgaactg gaaaactgca acgtatccgt atcaggacga caacattccc
     1741 atgctggatg cgctcatgca gcgtgccaag accaatgctg tcgatgccgt gatgattgcc
     1801 ggcgatgtgg tgtaccggga tggccagttc acgcgcgtgg atcgcgatgc gatcctcaac
     1861 gagatcgaac agacgctcgc gaagccgcgc aacgagcgca agcaggcgga gcgggaactg
     1921 ggcgcggcag tgtttcccta tgtaaaggcg ttctactccg actacttcgg cgaaaccccg
     1981 ccgcgtcagc cgttttacgc cccttcatcc cggaactgat ggatcgaatg acaacgcggg
     2041 tccttctgga aacgatcgct ccgttttcct gcgcctcctg gcggtcttcc agagggtctg
     2101 cgatgctgtg cttcctgtcg gaaagcgttc ggcctcaatt cgcgcgctgt cagatggata
     2161 acggtctatc gtccagcaga cggggcgccc ctccaacggc aagcatgcgc gcatggttcg
     2221 cggcgtccaa ctgacccgtc tcccgtctgc tcctgaacgg cgcgtcaatc gcttccgatc
     2281 ggagcctgtg agtcgttcct gaacacactc gcgagtcaat cgggcttctt cagtccactc
     2341 ggattctcag gtccaccgcg cgtcatccct tgatcagtcg gcgcgctcac accgcggtta
     2401 tcagtcgctg tgtcgcctcg tccggaggcc gccgacgcag aatggggagc gcgggttgcg
     2461 ccaggcatgc gtggtcgatc gccactctga gacgaccccg agtgggccgt ccctacagca
     2521 ctcgctcccg cctcacgggc cgcacctgta ttctgcgcag atccagtcgc cgcaaaagtc
     2581 gacagcagcg ctgcagctgc gaagcttttg aagaggactt tcattttcgt ctccgtacgc
     2641 gctttgcatt ggcagtactt tcatcttaga tcggctgaac gacaggacca gcatttttcg
     2701 ttacatccga cgaaagtcac aatcggcgca tcgttgtaat tattcgtctc gcttctgacg
     2761 ggtgcattct cttcgagtct gacggcaact cctggcggcc gcgtacgcca actctggatc
     2821 gcactctcga tgtagcccga acattcggca aaccgccgta tggtgacgta acggtgcctc
     2881 ggaagatcgt ctttacaata gaaacggtat tgtcatgttg ttgaaagtcg ggtgagcgaa
     2941 aacacataac gtaagcattc ctgcattatt cggcaagaaa atcccgcatt gtatttttgg
     3001 ccaagggttc ccatgcctcg aaagcctgct tgcgcgccgg ccacattccg gcaacgtgcc
     3061 agtaccctgc gaaccgctca acgccgcgag agtaaacctc ggtaatggcc gcgtaactca
     3121 agtaacgtgc tctgaaccta gactgtggtc aattcaacga acgactcgtt catgacctgg
     3181 caagcgcctc gccaactgtg ggaagtgagc caatgggata tatctatgcg gcagccaatc
     3241 cagccagtat ctcgtgtggc cttgattctt tcaccagcag tgggcgactc actgttgatg
     3301 atgacgatcg cgcacaacct tcagaaaaac tgcattgcag tcaccgtatt cggccaacat
     3361 acgcatgctc tgcgcgattg gtttccagct gtcgacatac gggccgagtt gggcgaagcg
     3421 catcttgcgg agaaactgca cgacttcgat acggtcattc aaatgcacag caatcgaccg
     3481 ttcgctggac tggatcgatt gcatccccga gtgatcacgc tcgaccacat cgtcagcgcg
     3541 cgttcctcag aatcgatggc agatagactt gcactattct gtcgcgatga gcttggtctg
     3601 acgaatggcg aaaaaagtaa tggcatcatg ccgccgccag gacttaacca ccgcaaatac
     3661 ccgtttcgag tcgccatcca tcccaccgca agcaccttgg acaaacgctg gcttgcatcc
     3721 cgcttcattc gtttggcaat ggaactgcgc gaccggggtt tcagcccgga gtttgtagtt
     3781 gcgccgcaag agcgtgcgga ctggaagcac gtcgagcgtt tcggcatcac tttgcccgat
     3841 ctctcttcgc ttgatcatgt cgccacatgg ctcttcgagt gcgggtggtt catcggcaac
     3901 gattcgggca tcggccatct cgcatcaagc ctgcagattc cgacactttc attgttcatg
     3961 cgccgtggta tcgcgcgcac ctggcgtcct ggctggggta ccggacgtgt gctgattggc
     4021 ggggcctacc tgcccacggg acgtttgaag gagcgctact ggaaatacct gttatccgtc
     4081 ggcaaagtaa cccaggcgtt cgagcaacta cgcaccaatc tcacatgaat ccattaccca
     4141 ttccccgcac gaccggagac ggaccgacgc gcgtcgccgt agtcatgtcg aacgcaattg
     4201 gcgacacttt ggtcttgatg gtcatcgtcc agaatcttct gcgaaatggt tttgatgtca
     4261 cggcattcgg tcatccggct tatagattgc ggcactggtt ccccaatgtc gcgattcgac
     4321 cgctgccgga caataaggat gccgatgcat tgttggcgcg gtacgacact gttcttcaaa
     4381 tgcatcggga tcagccaatt acacgcctga cagatgtcca cccttgcgtc gtctatctgg
     4441 acgagatcga attcggagat cgttcaggtt gtatggcgga acgcttcgcg gccttctgcc
     4501 gtgacgaact cggactatca aatgtcgatt tgtataatgg catcacgccc ccgccaacgc
     4561 tacgacaccg tcattacatt cgccgtatcg cgatccaccc cgaagccagc acagaggaca
     4621 agcgctggct gtcaggacga ttcgtgaagt tagcccacaa tttgcgtcaa cgcggctacg
     4681 acgtcaactt cgtgattgca ccgcacgaac gcggacgctg gtgcgaactg gaacggtgga
     4741 acatccctgc cccagaattt agcagccctc atgagctggc atgctggcta tacgaatccg
     4801 gctggtttat cggaaacgac tccggggtcg ggcatcttgc atcaaatctc agcattccaa
     4861 ccattagcct gttccgacga cgccgcgtat ccgaacgctg gcgtccggcg tggggaacag
     4921 tcaacgttgt tttaccctgg caatgggttc ccactgcgcg actcaaggag aaactctggc
     4981 gtcaaacact cacgtgcgcg cgcgtgttgt cggcgtttca gagtacggtt cgagatgacc
     5041 gcgacaccgc gaaacatgat tcaagcaagc gataaggcga ttttgtggtt ctgagaactg
     5101 cgggacctcg cgttgctgtt tgagccgcac tatacggaca ccatgggcgc tccaccggac
     5161 acaaccctgt ccggttgacg tatgaaggaa ttctttccct ggcgcgagga ttccgctctc
     5221 ctcaaacgca taccgtcaaa cgataccgtc acgctacttg acggtataga acagggccgt
     5281 ccgaggcggg aatcccgata ccgtcagcga taccgacatt cccgtttggt attgcccaga
     5341 tcacaacgga ccttatcgga caataactcc atgttttttt gaaagaaaaa tatggaaaac
     5401 cggacctccc tgaacgactt gggagcagtc tgaatcactc ccactcgatc gtcgccggcg
     5461 gtttccccga gatgtcataa accacccggt taatcccgcg cacttcattg atgatccggt
     5521 tcgacacatg ccccagcaat tcatgcggca gatgcgccca atgcgcggtc atgaagtcga
     5581 gcgtctgcac ggcgcgcagc gccacgacgt actcataggt gcggccgtcg cccatcacac
     5641 cgacgctctt cacaggcagg aacaccgcga acgcctggct cgtcaggtcg taccatgact
     5701 tgccggtttc cttgtcgatg aaggtgcgca gcgtctcgat gaaaatcgca tccgcgcgac
     5761 gcagcaggtc agcgaaatcg cggcgcactt cgccgagaat ccgcacgccc agacccgggc
     5821 ccgggaacgg atggcgatac accatggccg gcggcaaacc gagcttcacg ccgagttcgc
     5881 gcacttcgtc cttgaacagt tcgcgcagcg gctcgagcag cttcagattc agcgtctccg
     5941 gcaggccgcc cacgttgtgg tggctcttga tggtctgcgc ggccttcttg cccttgccgg
     6001 ccgattcgat cacgtccgga tagatcgtgc cttgcgccag ccacttcgcg tcggtcagct
     6061 tgttggcttc ggtctggaac acttcgacga attccgcgcc gatgatcttg cgcttcgctt
     6121 ccggatcggt cacaccggcc agcttcgaca ggaacacttc gctcgcgtcg acgtgaatca
     6181 ccttcacgcc gaggtgatcg gcgaaggtcg ccatcacctg ctcggcttca ttcaaacgca
     6241 aaaggccatg atcgacgaac acgcaggtca gctgatcgcc gatcgcgcga tgcagcagcg
     6301 ccgccgccac cgacgaatcc accccgcccg acaagcccag aatcacgtgc tcgttaccca
     6361 cctgctcgcg aatcttcgcg accgcttcgt cgatgtaatt gcccatttcc caatcgggcc
     6421 gggcgccgca gatcttcagc acgaagcgct cgagcatcgc gcggccttgc acggtgtgcg
     6481 tgacttccgg gtgccattgc agaccgtaga aatggcgctt ttcgtcggcc atcgccgcga
     6541 tcgggcacga ttccgtggat gccatcagct ggaagccctc aggcatttcc agcaccttgt
     6601 cgccgtggct catccacacc ttcagcatgc cgtgaccttc cggcgtggtg aagtcgctga
     6661 tgccttcgag caggctcgta tggttgcgcg cacgcacttc ggcataaccg aactcgcgca
     6721 ggtggccaat atcgaccttg ccgccaagct gctcggccat tgcctgcatg ccgtagcaaa
     6781 tgcccagcac gggcacgccg agttcgaaca ccgcttgcgg cacgcgcggc gtatccgttt
     6841 cggtgaccga gctcgggccg ccggagagga tcacgccctt cggcgcgaaa tcgcgaatga
     6901 acgacgcatc gacgtcgtac ggatgaattt ccgaatacac gttggcttcg cgaacgcgac
     6961 gtgcaatcag ttgggtgact tgcgaaccga agtcgaggat caggatcttg tcatgcatgg
     7021 cagcggacgg agtcaaaagt gaacggatac gggatgccgc gcacggagcg cggcgttgaa
     7081 ttcaaagcgg tccacgtggg gacgatcgaa catgttgcgt aacgatgaag cgagctggac
     7141 cggcggacgc cgcaggcatt gacctgcggc gcgcacgaac ggaatccggt agcttcggca
     7201 ggcgttctgg cgctcggcgc ctcagccatg aaacggcggc cggggcgcac cgggcgcgtc
     7261 ggcggcggag ttgcgggcct cgtcgcgcgc ggctgcggcc tcagcccgtg cggcgcgttc
     7321 agacgccgtc aggcccggtt cgtcgaccac cggatgaagc ggcgccgccg caacaggatc
     7381 caggggccgt gggaccggca taacaggctc gaccaccggc tcggcgtgat agaccggaaa
     7441 acccgccgtc acccggccgg cgttgcgccg gttggcagca tctttcgcgg catctttggc
     7501 agcctgcttt tcagcacgct cggcggccgc ggcttcaacc agccgcgccc tttcgcgccg
     7561 gcgcaccgcg cccgagaggc gcacaccgcc caggccgatg accagcaaga ccaccccggt
     7621 cagcaccgcg acgatttgcc cgaagccggt cagcgccggc gtatgcaagc caagcagcca
     7681 gaccagcatc agcacgccgg tcagccagtt cacatggcca acgaccttgg caaccgtcga
     7741 cgtgagcgaa ccgtcgagcg acgcatgcaa agccagccac gcgagcccga tcagcgccac
     7801 gccaaaccct tgcccgacca gagccggctg ggtttgcacc agtagcagcg cgttgtacaa
     7861 cgaagtccag ggcgtcagca gaaacagcag gccgaacgca agcagcagca tggcatcgag
     7921 gatgagtaca gcgcgcagca atggcttcat tggcgtttag tccacgtggt agttgggcgc
     7981 ttccttggtg atctgcacat catgcacatg cgactcgcgc aagcccgcgc ccgtgatctg
     8041 cacgaactcg gccttgtcgt tcatctccgc gatcgtgcgg cagccgcagt agcccatgct
     8101 ggcgcgcacg ccgccgatca gctggaacag gatcgcgttg accgagccct tgtaggcaac
     8161 gcggccttcg ataccttccg gcaccagctt gtcgatgttc gcggagttgt cctggaagta
     8221 gcggtctgcc gcgccgtcct tcatcgcgcc gactgaaccc atgccacggt acgatttgta
     8281 ctggcgcccc tggaacagga acacgtcgcc cggcgcttct tcggtgccgg cgaacatgct
     8341 gcccatcatc acggaattcg cgccggcggc cagcgccttg ctgacgtcac ccgagaaacg
     8401 cacgccgccg tcaccgacga ccggcacgcc cgtgcccttc agcgcttccg acacattggc
     8461 gatcgccgtg acctgcggca cgcccacgcc ggcgacgatc cgcgtcgtgc agatcgagcc
     8521 gggaccgata ccgaccttca cgccatccgc gccgtattcg acgagcgcct tggccgccgc
     8581 ggcggtggcg atgttgccgc cgatcacttc gacatgcggg aacttacgct tgacccactt
     8641 gacgcgctcg agcacgccct tgctgtggcc gtgcgccgtg tcgacgacga tcacgtccac
     8701 gcccgcctgc accagcagct cgacgcgttc ttcgttgtct tcgcccacgc cgaccgccgc
     8761 gcctgcgcgc agcttgccgt gttcgtcttt acacgcgtcc gggtgttcgg tttgcttcgt
     8821 gatgtctttc accgtcatca ggccgcgcag ttcgaatgcg tcgttgatga ccagcacgcg
     8881 ctcgaggcgg tggctgtgca tcagcgcctt ggcttgcgcg agcggcgtgc cttccttgac
     8941 ggtgacgagg cgctcgcgcg gcgtcatgat gttgcgcacc ggctcgtcca gacgctcttc
     9001 gaaacgcagg tcgcggttcg taacgatacc gatcagttgc gcgccttcga cgaccgggaa
     9061 gcccgaaata ccatgctggc gcgacagcgc gatgacgtcg cgcaccttca tttgcggcgg
     9121 caccgtgatc ggatcgcgca cgacgcccga ttcgaagcgc ttgaccttgg ccacttcacg
     9181 cgcctgttcg gccgcggtga gattcttgtg gatgatgccc acgccaccca tttgcgccat
     9241 cgcaatggcg aggcgtgctt cggtgacggt gtccatggcg gcggacacaa gcggcatatt
     9301 cagggagatg ttgcgggtca gccgggtctt gaggctggtg tcgcgcggca gaacatcgga
     9361 gaaggccggg acgaggagca cgtcatcgaa cgtgagtgct gtttggatca gacgcatggc
     9421 aaatcctata ggcgcaaaag cgaattat
//
LOCUS       DATASH010000241         6427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_624710,
            whole genome shotgun sequence.
ACCESSION   DATASH010000241 DATASH010000000
VERSION     DATASH010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6427
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624710"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="VHV99_06755"
     CDS             complement(<1..695)
                     /locus_tag="VHV99_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3635177.1"
                     /translation="MQDIGISFGGVPALRAANLSVAAGEVHALIGQNGAGKSTMIKIL
                     TGAYRRGSGSVRFEGREVDFRTPKQAREAGISTIYQEINLVPFRSVAENIFLGREPRR
                     FGLIDWRAVQQRAAALLESFGLQIDVKKPVGRYSTAIQQMVALARAVSSDAKMVIMDE
                     STSSLDEREVELLFTVVRKLRDDGRAVIFVSHRLDELYALCDRVTVMRDGQTVAQSTL
                     ADMDKLQLVTTMLG"
     gene            complement(1131..2150)
                     /locus_tag="VHV99_06760"
     CDS             complement(1131..2150)
                     /locus_tag="VHV99_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3635178.1"
                     /translation="MASLNTQLRKHARKQQIRPLAGSLLALAIGFGVATAHADDALPK
                     LPNKTPLKVGFAQTESNNPWRLAETRSFKDIAAKCGWQLVMTDANSSNSKQVSDIQNM
                     IAQHVDLLVFPPREEKPLAPIVLQAKKAGIPVILVDRDVDQSVAKAGRDYITFIGSDF
                     IDQGHRAADWLIKATGGKAKIIELEGTTGASAANDRKKGFDEIIAKNPGMTIIASQSG
                     DFARDKGRQVMETLLQSHPDVTAVYAHNDEMALGAIAAIKAAGKQPGKDIQIVTIDGT
                     KGGMDAIAAGELGASVQSSPFFGPLACDVAQRYAKGEKIPTWVKVSDKFYDKSNVQQN
                     MQYGY"
     gene            complement(2268..3320)
                     /locus_tag="VHV99_06765"
     CDS             complement(2268..3320)
                     /locus_tag="VHV99_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX3635179.1"
                     /translation="MTDIAKLTGVSQSTVSLVLNNATGAKFSESTRNKVLKAAHDLGY
                     RLSLREPVSASNDERNLIVYLADEISTSPHPVVNIDGARDAAYASGKMLAVYSTHGNA
                     DIEKQVLDATLSNPHVFGVVYATVYTRKVTLPAALSQVPTVLLNCYTSEGGCSSVVPA
                     EVAGGHLATDYLLQSGHRRIGYINGEPWQDASKDRLKGYRTALATADLPFAPELVRDG
                     DWSSGLGFELTLSLMREANPPTAIFCANDLTAIGAIEALKQLGHRVPEDVSVLGYDDQ
                     EIARHTHPPLSTVVLPNYELGRWAVETLLQEEQNRAAGAPVRHRMVKLDGPLVERASV
                     RVITEAKKPIINIISD"
     gene            3658..4638
                     /locus_tag="VHV99_06770"
     CDS             3658..4638
                     /locus_tag="VHV99_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007582144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HEX3635180.1"
                     /translation="MRDVSLNSIRPLATELPWLDDPVIARTLVTLKAGAMRVVIAPEV
                     GGALAAYFEITSAGPLHWLRPATRAAFDERDPLRMASFPLFPYCNRIRDARFQFDGDT
                     VDLSGNDLHFAHALHGNAWRHPWNVGARTESSVELHFEHEPDARQPGDWPFRYRARQR
                     IELSDGALRITLSAQNLGDRPMPFGMGHHPYYPRTAQTRVYAEVQGMWHADADVLPTH
                     LGPHPAVEALRHGMSPDAFDLDNNFANWSREATIAWPDEHRQLTMTADAPFDHMVVFA
                     PANDTQLCVEPVTNTTDCFNTVGMQEHVGGCVLQPGEEIAAALNWTPGRG"
     gene            complement(4640..5437)
                     /locus_tag="VHV99_06775"
     CDS             complement(4640..5437)
                     /locus_tag="VHV99_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013337782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX3635181.1"
                     /translation="MRNPEELIRESAAPKDFIAVSWNLHKGRTPLGFQAWQAMQRWVQ
                     STHADAYFLQEAMARRMPSPILASTFGSPLADPLSDVWHCQATEIARAAELEIALGPN
                     VFKPSWRHGNAILSPHPLDLGGRWDISAHRFEKRGLLVARATFGGHSVTLLCAHLALT
                     RSARLRQMNWIAHWIAKEAPEGPLVLAGDFNDWRNDSVPLFAEHGLQEVATLLGEAGR
                     TFPAFSPALALDKMFVRGMEPVEWIQPTQETAWLSDHLPYMARLRVE"
     gene            complement(5577..6083)
                     /locus_tag="VHV99_06780"
     CDS             complement(5577..6083)
                     /locus_tag="VHV99_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635182.1"
                     /translation="MWKTSIVAVLAAVLFGTSLLANAQQGSAQQAVQWQLQVMRDGQQ
                     IDSFDGTTTVGQARTDTHHKMVQHNVGCKDQPGGSIDLARTLTISPLQADANAVTLSI
                     EAQETFEEDAARQTDTGCKLPPQPRQVSASHPGLKVAAGQWASWTIVDKDPNLVYRVR
                     ASLANSAN"
BASE COUNT         1053 a         2023 c         2058 g         1293 t
ORIGIN      
        1 ccgagcatcg tcgtgacgag ctgcagtttg tccatgtccg ccagcgtgct ttgcgcgacc
       61 gtctggccgt cgcgcatcac ggtgacgcgg tcacacagtg cgtagagttc atcgagccga
      121 tgcgacacga agatcaccgc gcggccgtcg tcgcgcagct tgcgtaccac ggtgaaaagc
      181 agctccactt cgcgttcgtc gagcgacgag gtggattcgt ccatgatgac catcttcgcg
      241 tcggacgaca ccgcgcgcgc gagcgccacc atctgctgga tcgctgtcga atagcggccg
      301 accggcttct tcacatcgat ctgcaaaccg aacgattcga gcagcgcggc agcgcgttgc
      361 tgcaccgcgc gccagtcgat caggccaaag cggcgcggct cgcggccgag gaaaatgttc
      421 tccgccaccg agcggaacgg caccagattg atctcctgat agatcgtgct gatgccggct
      481 tcgcgcgcct gcttgggcgt gcggaaatcg acttcgcgtc cttcgaagcg cacgctgccc
      541 gagccgcgcc gatacgcgcc ggtcaggatc ttgatcatgg tcgatttgcc cgcaccgttc
      601 tgaccgatca gcgcgtgcac ttcgccggcg gcgacgctca gattggcggc gcgcaacgcg
      661 ggtacgccgc cgaagctgat gccgatgtcc tgcatctcga gcagcggcga acgggtaagg
      721 ggagagtgtg gcgtctcttt gccggattcc gtcacgggtg tcctcctgat gcaggtgctg
      781 cttgatttgc cgcgtgcgcg ccaggcgttg cacgtgcgag ccggtgcggc ccttcgttcg
      841 ggctctgctg cgggcgctgt ggtcgagcca tgttacgccg cataaaagcg aagcgacggt
      901 tcgtgctgcc acgaacgcgt cgctccgaac ccttcccgtc gcccgccccc cgaggagaca
      961 gattcggggc ggggacgtcc tgcgaggcaa tcccccgact ccgatgtctc agacacgacg
     1021 tgcttcgggg cgggagactt ccttcttcgg ggcgccgctc gaacgcgtga acgctccact
     1081 acggcaaagc gctcggactt caaaaccgtg catacagcgg cgcttcgcca tcaatagccg
     1141 tactgcatgt tctgctgcac gttgctcttg tcgtagaact tgtcggacac cttgacccac
     1201 gtcgggatct tttcgccctt cgcgtaacgc tgtgcgacgt cgcaggcgag cgggccgaag
     1261 aacgggctcg actgcacgct cgcgccgagt tcgccggcgg cgatcgcgtc catgccgccc
     1321 ttggtgccgt cgatcgtgac gatctggatg tccttgcccg gctgcttgcc ggctgccttg
     1381 atcgcggcaa tcgcgccgag tgccatttcg tcgttgtgcg cgtagacggc ggtcacgtcc
     1441 ggatgcgact gcaacagcgt ctccatcacc tgacgaccct tgtcgcgtgc gaagtcgcca
     1501 ctttgcgagg cgatgatcgt catgcccgga tttttcgcga tgatttcgtc gaagcccttc
     1561 ttgcgatcgt tcgcggcgga tgcgccggtg gtgccttcga gttcgatgat cttcgccttg
     1621 ccgccggtcg ccttgatgag ccagtcagcg gcgcgatggc cctgatcgat gaagtccgag
     1681 ccgatgaagg tgatgtagtc gcggcccgcc ttggcgaccg attgatcgac gtcgcggtcg
     1741 accaggatca ccggaatgcc ggccttttta gcctgcagca cgatcggcgc aagcggcttt
     1801 tcttcgcgcg gcgggaacac cagcagatcg acgtgctgcg cgatcatgtt ctggatgtcc
     1861 gagacctgtt tggagttcga gctgttggcg tcggtcatga ccaactgcca gccgcacttc
     1921 gcggcgatgt ccttgaagct cctggtttcc gcgagacgcc acgggttgtt gctttcggtc
     1981 tgcgcgaagc cgaccttcag tggagtcttg ttcggcagct tcggcagtgc gtcgtcagca
     2041 tgcgccgtgg cgacgccgaa accgatggcc agagccagca gcgaacctgc cagcggacga
     2101 atctgctgct tgcgcgcgtg cttgcgcaac tgcgtgttga gcgacgccat tgtcttcctc
     2161 cagtacctgg tgaggtaggt gtatttgctt ttagtgtttc tcctgccggc cggcgctgtt
     2221 ccgccatgac cggacgtgcc gattatttca cttggaatta ttatcggtca atcactaata
     2281 atattaatta ttggcttttt tgcctcggta attaccctga ccgaagcgcg ttcgacaagc
     2341 ggaccatcga gcttgaccat gcgatgccgc accggtgcgc cggcggcgcg gttttgctcc
     2401 tcttgcagca aggtttcgac ggcccagcgc cccaattcgt agttcggcaa caccacggtc
     2461 gagagcggcg ggtgcgtatg gcgggcgatt tcctggtcgt cgtaaccgag caccgacacg
     2521 tcttcgggga cgcgatggcc gagctgcttc aacgcctcga tcgcaccgat ggcggtcagg
     2581 tcgttggcgc aaaaaatcgc ggttggcgga ttggcttcgc gcatcagcga gagcgtcagt
     2641 tcgaaaccga gaccggagct ccagtcgccg tcgcgcacca gttccggcgc gaacggcaga
     2701 tcggcggtgg cgagcgcggt gcggtagccc ttgaggcggt ctttcgaggc gtcctgccac
     2761 ggttcgccgt tgatatagcc gatccgccga tgacccgact gcagcagata gtcggttgcc
     2821 agatggccgc ccgcgacctc ggcgggcacc accgatgagc acccgccttc gctcgtgtag
     2881 cagttcagca gcaccgtggg cacctgagag agcgccgccg gcagcgtgac cttgcgggtg
     2941 tagacggtcg cgtagaccac gccgaataca tgcgggttcg acagggtcgc gtcgagcacc
     3001 tgcttttcga tgtcggcatt gccgtgtgtc gagtagacgg cgagcatctt gccgctcgca
     3061 taggcggcgt cgcgcgcgcc gtcgatattg acgaccggat gcggactggt cgagatttcg
     3121 tcggccagat agacgatcag gttgcgttca tcgttcgacg ccgacaccgg ttcgcgcagc
     3181 gaaagccggt agccgaggtc gtgcgcggcc ttcagcacct tgttgcgggt gctctcggag
     3241 aatttcgcgc cggtcgcgtt gttcagcacc agcgagacag tcgattgcga cacgccggtg
     3301 agcttggcga tgtcggtcat ggtgggacgg cgttgagtcg attttttcat tgtgcgggcc
     3361 gcgctgcggc ggcgcgaaag caggagcatg ccggtggcat gagtggtcct gacggtacca
     3421 ctaataatat gcgcgcagca acgcgcgcta acccgccatt gggtgttctg gcaaggcatt
     3481 ggcgggctaa gtttggcaaa tttattacta ataatattga ccgatgtgct ttgtcgtgcg
     3541 attctcggtc gcgcggaccc tgacagttcg ccggccgcgt gcagtacgcg gcacgtcata
     3601 ggagacattg atgcgcgttc atgccgccct ccgcctgagg cgccgccttt tctgaccatg
     3661 cgcgacgttt ccctgaattc gatccggccg ctcgcgaccg agctcccgtg gctcgacgac
     3721 cccgtgatcg cacggacgct tgtcacgctg aaggcgggcg ccatgcgagt cgtgatcgcg
     3781 cctgaggttg gcggcgcgct ggcggcttac ttcgagatca cgtccgcagg cccgctgcac
     3841 tggctgcggc cggccacccg agcggcgttc gacgaacgcg atccgctgcg catggcgagc
     3901 tttccgctct ttccttactg caaccggatt cgcgacgcgc gcttccagtt cgacggcgac
     3961 acggtcgatc tgagcggcaa cgacctgcat tttgcgcacg cactacacgg caatgcgtgg
     4021 cggcatccat ggaacgttgg ggcgcgcacg gaaagttcgg tggaattgca cttcgaacac
     4081 gaaccggatg cgcggcagcc cggcgactgg ccgtttcgtt atcgcgcgcg gcaacgcatc
     4141 gagttgagcg atggcgcgct gcgcatcacg ttgtccgcgc agaacctggg cgaccggccg
     4201 atgcccttcg gcatgggcca tcacccgtac tatccgcgca ccgcacagac ccgcgtttac
     4261 gccgaggtgc aaggcatgtg gcatgccgac gccgacgtgc tgcccacgca tcttggcccg
     4321 catcccgcgg tcgaggcgct gcgtcacggc atgtcgcccg acgcgttcga tctcgacaat
     4381 aattttgcca actggtcgcg cgaggcgacg atcgcatggc cggacgaaca ccgtcagctg
     4441 acaatgaccg ccgatgcgcc cttcgatcac atggtggtat ttgcgcccgc caacgacaca
     4501 caactctgcg tggaaccggt gacgaacacc accgattgct tcaacacagt cggcatgcag
     4561 gagcacgtag gcggctgcgt gttgcagccg ggcgaggaga tcgccgcggc gttgaactgg
     4621 acgccaggcc ggggctagct cactcgaccc tcaagcgcgc catatatggc aaatgatccg
     4681 acagccacgc agtctcttgc gtcggctgga tccattcgac cggctccatg ccgcgcacga
     4741 acatcttgtc gagcgcgagc gccggcgaga aggccgggaa cgtgcggccc gcttcgccga
     4801 gcagcgtcgc cacttcctgc agaccgtgtt cggcgaagag cggaaccgag tcgttgcgcc
     4861 agtcgttgaa gtcgccggcc agcaccagcg gaccttccgg cgcttccttc gcgatccagt
     4921 gcgcgatcca gttcatctgc cgcaagcgcg cggaacgcgt gagtgcgaga tgcgcgcaca
     4981 gcagcgtgac cgaatgaccg ccgaaggtcg cccgcgccac cagcagtccg cgcttttcga
     5041 agcggtgcgc ggagatgtcc cagcggccgc cgaggtcgag cggatgcggc gacaaaatcg
     5101 cgttgccatg ccgccatgac ggcttgaaca cgttcggccc aagcgcgatt tcgagttcgg
     5161 ccgcgcgtgc gatttcagtg gcctggcaat gccagacatc gctcaatgga tcggcgagcg
     5221 gtgagccgaa agtgcttgcc aggatcggcg agggcatgcg ccgcgccatc gcttcctgca
     5281 aaaaatacgc gtccgcgtgg gtcgactgga cccagcgctg catggcctgc caggcctgaa
     5341 agccgagcgg tgtgcggccc ttatgcaggt tccagctcac cgcaatgaaa tccttgggcg
     5401 ctgcgctctc gcggatcaat tcttctgggt ttcgcatgcc gcgttgtcca cgtgttctgt
     5461 ttgggggctt cggttgttcg cttcagtatc agttggcgcg attcgcatcc atccgcgctg
     5521 cttggcgcta gttcgtgcag ttcgcatcca tccgcactgg tttgcgccgc cttgcgctag
     5581 ttcgcgctgt tcgcaaggct tgcacgcacg cgatagacca ggttcggatc cttgtccacg
     5641 atcgtccagc tggcccactg cccggccgct accttcagac ctggatggct cgcgctgacc
     5701 tgacggggtt gcggcggcag cttgcagccg gtatcggttt gccgcgcggc gtcttcctcg
     5761 aaggtttcct gggcttcgat cgacagcgtg accgcatttg catccgcctg cagcggcgag
     5821 atggttagcg tgcgcgccag atcgatgctg ccgccgggct ggtctttaca gccgacgtta
     5881 tgctgcacca tcttgtgatg cgtgtccgtg cgcgcctgac cgacggtggt ggtgccgtcg
     5941 aatgaatcga tctgctggcc gtcgcgcatc acctgcaact gccattgcac cgcctgttgc
     6001 gccgaccctt gttgcgcgtt cgcgagcagc gaagtgccga acagcacggc ggctagcacg
     6061 gcaacgatac tggttttcca catcgaagga ttctccggtc actgcgtccg gcagcgttgt
     6121 cagagggcca tcttacgcct gatgggctga acgcttttct gacacttcaa acggggccgg
     6181 gttcggcaat acagcatttc ggatagggcg atgaatgccc cgggggcact tccgcagagg
     6241 cgtccatcaa gggggacttt cttcggggcg tccctcaagg gagcttcccc tggggcgctc
     6301 gcccaagagg acttgcccgg ggcgctcccc gaagaggggt tgctcggggc ttgctccgag
     6361 aggactcact cggggcgctc cccgaagagg acttactcgg ggtgtcctcc aagagcactt
     6421 tctcggg
//
LOCUS       DATASH010000242         2640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2406930,
            whole genome shotgun sequence.
ACCESSION   DATASH010000242 DATASH010000000
VERSION     DATASH010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2406930"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2185
                     /locus_tag="VHV99_06785"
     CDS             <1..2185
                     /locus_tag="VHV99_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3635183.1"
                     /translation="RTAAVARAYGVACDVISPREAGDLWPVMRTDDLLGAVWLPGDGK
                     ANPTDLTQALARGARTRGARIVENTRVTAIHTRTALEDKANGTTGAREVSGLAWRNKD
                     GEEGTLGADIVVNCAGQWAKAVGRLCGVTVPLHSAEHYYIVTERIAGVHPDLPVMRDP
                     DGFIYFKEEVGGLVMGGFEPNAKPWGMNGIPDNFEFQLLPDDWDQFEILMENALQRVP
                     ALETAQVKQFYNGPESFTPDNNFMLGEAPELRRFFVGAGFNSMGIASAGGAGMALAEW
                     IVAGEPTMDLWPVDIRRFARFNGNDTWLHDRVKETLGLHYAMPWPNRELDSARPFRRS
                     PLYALLRDDGACFGSKMGWERANFFAPTPEQAQIDYAFGKQNWLPWSGAEHRACREGV
                     ALFDMTSFSKFLVKGRDAQSVLQGIVANDVAVPPGTTVYTGMLNERGTYESDFTLTRL
                     ADDQYLIVTGSAQTTRDFDTIEKAIPHDKHCTLVDVTGQYAVLAVMGPRSRELLQSVS
                     KADWSNAAFAFGQSREVDLGYATVRATRLTYVGELGWELYVPVEFAVGVYETLHAAGK
                     AFGLVNAGYYAIDSLRIEKGYRAWGRELTPDTNPFEAGLSFACKLDKDIPFRGRDALL
                     KLRAQPPRRRMVVLTADGAAERMLWGGEAILRDGKPVGFVSSAAFGHTLGCPVAMGYI
                     NNPDGVADAAYLNSGRYMIDVAGELLPATVHLKAPYDPRSERVKG"
     gene            complement(2182..>2640)
                     /locus_tag="VHV99_06790"
     CDS             complement(2182..>2640)
                     /locus_tag="VHV99_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEX3635184.1"
                     /translation="FIGVGMVATVSHAHDTLIAIHKPLGIALLLLVIVRAAVRIKRGS
                     PPLPDDMPVSQRIAAKASHWVLYALMAAMPLIGWAMLSAAGYPITLYGPLHLPPIAPH
                     NNELFAALRALHSGLAFALFATVLLHLAAALFHGLIRRDGVVSSMARGGR"
BASE COUNT          504 a          850 c          864 g          422 t
ORIGIN      
        1 acgcaccgcc gccgtcgcgc gcgcctacgg cgtggcgtgc gacgtgatta gcccgcggga
       61 agccggcgat ctatggccgg tcatgcgcac cgacgacctg ctcggcgccg tgtggctgcc
      121 cggcgacggc aaggcgaatc ccaccgatct gacccaggcc cttgcccgtg gcgcacgcac
      181 gcgcggcgca cgcatcgtcg agaacacccg cgtcacggcg attcacacgc gcaccgcgct
      241 cgaagacaag gcaaacggca cgaccggcgc acgcgaagtc agcggcctcg cgtggcgcaa
      301 taaggacggt gaagaaggca cgctcggcgc ggacatcgtc gtcaactgtg cgggccagtg
      361 ggccaaggcg gtcggccgtc tatgcggcgt gacggtgccg ttgcactcgg ccgagcacta
      421 ctacatcgtc actgagcgga tcgccggcgt gcatccggac ctgccggtaa tgcgcgatcc
      481 ggatggcttc atttacttca aggaagaagt gggtggcctc gtgatgggcg gcttcgagcc
      541 gaacgcgaag ccgtggggca tgaacggcat cccggacaat ttcgaattcc agttgctgcc
      601 cgacgactgg gatcagttcg aaatcctgat ggaaaatgcg ctgcagcgcg tgcccgcgct
      661 cgaaacagcg caggtcaagc agttctacaa cgggccggaa tcgttcacac cggacaacaa
      721 tttcatgctc ggcgaagcgc cggagttgcg gcgctttttc gtcggcgcgg ggttcaattc
      781 gatgggcatc gcatcggcgg gcggcgcggg catggcgctc gccgaatgga tcgtcgcggg
      841 cgaacccacg atggacctgt ggccggtcga catccgccgc tttgcgcgct tcaacggaaa
      901 cgacacgtgg ctgcacgatc gcgtcaagga aacgctcggc ctgcactatg cgatgccctg
      961 gccgaatcgc gaactcgaca gcgcgcggcc gtttcgccgc tcgccgctgt atgcgctgct
     1021 gcgtgacgac ggtgcgtgtt tcggcagcaa gatgggctgg gagcgcgcca attttttcgc
     1081 gccgacgcct gaacaggctc agatcgacta cgcgttcggc aagcagaact ggctgccgtg
     1141 gagcggcgcc gaacatcgcg cgtgccgcga aggcgtcgcg ctcttcgaca tgacgtcgtt
     1201 ctccaaattc ctcgtcaagg gacgcgatgc acaaagcgtg ctgcaaggca tcgtcgccaa
     1261 tgatgtagcc gtgccgccag gtacgacggt gtacaccggc atgctcaacg aacgcggcac
     1321 ctacgaatcg gatttcacgc tcacgcgcct cgctgacgat caatacctga tcgtcaccgg
     1381 gtcggcgcaa accacgcgcg atttcgacac catcgaaaag gccattccgc acgacaaaca
     1441 ctgcacgctg gtcgacgtca ccggccaata cgcggtactc gcggtgatgg gcccgcgttc
     1501 acgcgagctg ctgcaaagcg tgtcgaaggc ggactggagc aatgcggcgt ttgcgttcgg
     1561 ccaaagccgc gaggtcgatc tcggctacgc gacggtgcgc gccacccgcc tcacctacgt
     1621 cggcgaactc ggctgggaac tgtacgtgcc ggtggaattc gcggtgggtg tgtacgaaac
     1681 gctgcacgcc gcgggcaagg catttggcct cgtcaacgcg ggctactacg cgatcgactc
     1741 cctgcgcatc gagaaaggct atcgcgcatg gggccgcgaa ctgacgccgg acacgaatcc
     1801 gttcgaagcg ggcctgtcat ttgcctgcaa gctcgacaag gacatcccgt tccgcggccg
     1861 cgatgcgttg ctgaaattgc gcgcccaacc gccgcgccgc cgtatggtcg tactcaccgc
     1921 cgacggcgcc gccgagcgca tgctgtgggg cggcgaagcg attctgcggg acggcaagcc
     1981 ggtcggcttc gtgagttcgg cggcgttcgg acacacgctg gggtgtccag tggcgatggg
     2041 gtatatcaac aacccggacg gcgttgccga tgcggcgtat ctgaacagcg gccgatacat
     2101 gatcgacgtc gccggcgagc tgctgccggc gaccgtgcat ctgaaggcgc cttatgatcc
     2161 gcgttcggaa cgggtgaagg gctaacggcc gccccgcgcc atactcgaaa ccacgccgtc
     2221 gcggcggatc aagccatgaa acagcgccgc cgcgagatgc agcagcaccg tggcgaacag
     2281 cgcgaacgcg agcccgctgt gcagcgcgcg cagcgccgcg aagagctcat tgttatgcgg
     2341 tgcgatcggc ggcaaatgca gcggcccata tagcgtgatc ggatatcccg ccgcggacag
     2401 catcgcccaa ccgatcaacg gcatcgccgc catcagtgca taaaggaccc aatgcgaggc
     2461 cttggcggcg atgcgctgtg acaccggcat atcgtcgggc aacggcggac tgccgcgttt
     2521 gatgcgcacc gccgcccgca cgatgaccaa cagcagcaac gcaatgccaa gcggtttgtg
     2581 aatcgcgatc agcgtgtcat gcgcgtgcga taccgttgcg accatgccca cgccaatgaa
//
LOCUS       DATASH010000243         4593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3198172,
            whole genome shotgun sequence.
ACCESSION   DATASH010000243 DATASH010000000
VERSION     DATASH010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3198172"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..158)
                     /locus_tag="VHV99_06795"
     CDS             complement(<1..158)
                     /locus_tag="VHV99_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3635185.1"
                     /translation="MQAIRLKHRERRPASFYWLALLFGLFVLFLYGPVITIFILSFQG
                     PEGGLTFP"
     gene            complement(161..1075)
                     /locus_tag="VHV99_06800"
     CDS             complement(161..1075)
                     /locus_tag="VHV99_06800"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3635186.1"
                     /translation="MATIDLPSHAQNEPVEHRRAPAWLQATPLSLTFLLFFIVPLVIT
                     LIVSFWDFNEYQIIPAFTLKNYAAIFNGCSSMTDVCVTFKTYWSTLRFCAIVWAVTLL
                     LGFSIAYFLAFHVRTTSMQTILFLVCTIPFWTSNVIRMISWMPLLGRNGLVNQALLGA
                     HLIDQPLEWLLYSNFSVVLAFVHLYTFFMIVPIFNAMMRIDRALLEAARDAGASGWQV
                     IRDVVLPLSKTGIVIGSIFVITIVMGDFLTVGVMGGQQIASVGKIIQVQTGYLQFPAA
                     AANAIILLAVVLMIVWGLTRVVDIRKEL"
     gene            complement(1208..2503)
                     /locus_tag="VHV99_06805"
     CDS             complement(1208..2503)
                     /locus_tag="VHV99_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PotD/PotF family extracellular solute-binding
                     protein"
                     /protein_id="HEX3635187.1"
                     /translation="MSEAYESPAAAASDLKEKGLSRRTFIKGAVAAAGIAGFPYVHAQ
                     EKITLRYLGTAVNQSADIAKKFKEDTGIDIQYVPVTTDDVAKRIITQPNSFDIVDTEY
                     FALKKLIPAGTLAGMDAKRIKLADKITPVLTRGEVNGKKIGDQGTAPKKVMFLTGPRS
                     TEFSATPTEWMTLIPTTYNADTLGIRPDLIKRPIDSWAELLNPQFKGKAALLNIPAIG
                     IMDSAMAIEAMGHMKYGDKGNMTKAEIDQTIKILIEVKRAGQFRAFWKDFNESVNLMA
                     SGEVVIQSMWSPAVTKVRTMGVACKFQPLKEGYRSLASGFGFPRSLQGKKLDAAYEFV
                     NWFQDGWAGAYLMRQGYYSGVLETAKTHMQPYEWDYWIEGKAAAQDIKAPDGQLLEKA
                     GTVRDGGSYNQRMGAIACWNAVMDENIYMVQKWNEFIAA"
     gene            complement(2654..3712)
                     /locus_tag="VHV99_06810"
     CDS             complement(2654..3712)
                     /locus_tag="VHV99_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3635188.1"
                     /translation="MIQPASMVLETTAADIELVHVSKQYGDSLAVDAIDLRIAGGQYC
                     CLLGPSGCGKTSTLRMIAGHESVTEGDILIAGRNVTREEPAARGTAMVFQNYALFPHL
                     SALDNVAFSLKMRGIAKDVRRKRAGELLELVAMSAYAERKPAQLSGGQQQRVALARAL
                     LNQPGCLLLDEPLSALDPFLRVQMRAELKRWQKELGITFVHVTHSQEEAMALADLVVV
                     MSHGRIQQSGTPHEVFNRPRTEFVARFLGGHNVFNTNGRSFTVRADHLRVVPHGVTPV
                     QTATRENGLTQIGGVPCTVREAEYQGTHLRMTLDPLDGSPELISLVSDGDYDPARLGP
                     DARVVVWWNEQDAHPLAA"
     gene            4028..>4593
                     /locus_tag="VHV99_06815"
     CDS             4028..>4593
                     /locus_tag="VHV99_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007584722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX3635189.1"
                     /translation="MNDTRNMPAAENRARQPIATHGRFAYRPITDSDAFRWPGDSGLA
                     VYLGFNIEHFAFGEGLGAALGPISPQPDVLNHSWREYGNRVGAWRCIELFDQLDMPAG
                     TLINTALYDHCPELIAACVARGDELIGHGHTNAHRQSDLDENGERELLLHCRERIAEA
                     SGTPPQGWLSPWISESHLTPDLLAETGYR"
BASE COUNT          908 a         1414 c         1366 g          905 t
ORIGIN      
        1 atcggaaacg tgaggccgcc ttccggtccc tgaaaagaca gaatgaaaat ggtaatcacc
       61 ggtccgtaca gaaacagcac gaacaggccg aacagcagcg cgagccagta aaagctggcc
      121 ggtcgacgct cccgatgttt aaggcgaatg gcctgcatgt tcacagctcc ttgcggatat
      181 cgaccacccg cgtcagaccc cacacgatca tcaacacgac agccagcaga atgatcgcgt
      241 tagcggccgc cgcgggaaat tgcaggtagc cggtctgtac ctgaatgatc ttgccgaccg
      301 atgcaatctg ctggccgccc attacgccga cagtcaggaa atcgcccatc acgatcgtga
      361 tcacgaagat cgagccgatc acaatgccgg ttttcgacag cggcaacacc acgtcgcgaa
      421 tcacttgcca gccgctcgcg ccggcatcgc gcgccgcttc gagcaacgcg cggtcgatgc
      481 gcatcatcgc gttgaagatc ggcacgatca tgaagaacgt gtagaggtgc acgaacgcca
      541 gcacgaccga gaagttcgaa tacagcaacc actcgagcgg ttgatcgatc agatgcgcgc
      601 ccagcagcgc ctgattgacg agcccgttac ggccgagcaa tggcatccac gagatcatgc
      661 ggatcacatt cgacgtccag aacggaatcg tgcagacgag gaacaggatt gtctgcatgc
      721 tcgtcgtgcg cacgtggaac gcgaggaagt aagcgatcga aaagcccagc agcaaggtca
      781 cggcccatac gatcgcgcag aacctgagcg tcgaccagta tgtcttgaag gtgacgcaga
      841 cgtccgtcat cgacgaacag ccgttgaaaa tcgccgcgta gtttttcagc gtgaaagccg
      901 ggatgatctg gtattcgttg aagtcccaga aactgacgat caacgtgatg accagcggaa
      961 cgatgaaaaa cagcaggaac gtcagcgaaa gcggcgttgc ctgcaaccat gccggcgcac
     1021 gccggtgttc gacgggctca ttctgcgcgt gagagggaag gtcgatcgtt gccatgggcg
     1081 aatatccagg gaagcactgc ggaagcattg cgctgcggca tgaccacgtc agcgaaaaac
     1141 tgccggcatg tcatgcaccg gcagtccggc atgcggctcg gccgctgaag ccacgctgag
     1201 gccaggttca agccgcgatg aactcgttcc acttctgaac catgtagatg ttctcgtcca
     1261 ttaccgcatt ccagcaggca atcgcgccca tgcgctggtt gtatgagccg ccgtcgcgca
     1321 cggtgccggc tttttcgagt aactgcccat cgggcgcctt gatgtcctgc gcggcagctt
     1381 tgccttcgat ccagtagtcc cattcgtagg gctgcatgtg ggtcttcgct gtctcgagga
     1441 cccccgagta atagccctgt cgcatcaggt aggcgccggc ccagccgtcc tggaaccagt
     1501 tgacgaactc gtacgcagcg tccagcttct tgccttgcag cgagcgcgga aaaccgaaac
     1561 ctgaggccaa cgagcgatag ccttccttga gcggctggaa tttacaggcg acacccatgg
     1621 tgcgcacttt ggtcacggcc ggcgaccaca tcgactggat caccacctcg cccgaagcca
     1681 tcaggttcac gctttcgttg aagtctttcc agaacgcgcg gaactggccg gcgcgcttga
     1741 cttcgatcag gatcttgatg gtctggtcga tttcggcctt ggtcatgttg cctttatcgc
     1801 cgtatttcat gtggcccatc gcctcgatcg ccatcgccga gtccatgatg ccgatcgccg
     1861 gaatgttcaa cagcgccgcc ttgcccttga attgcggatt gagcagttcg gcccagctgt
     1921 cgatcgggcg cttgatgagg tcgggacgga tgccgagtgt gtccgcgttg tacgtggtcg
     1981 gaatcagcgt catccattcg gtcggtgtcg cggagaattc ggtggagcgc gggccggtca
     2041 gaaacatcac ctttttcggc gcggtgcctt gatcgccgat cttcttgccg ttcacttcac
     2101 cgcgtgtcag caccggcgtg atcttgtcgg cgagcttgat gcgtttcgcg tccatgcccg
     2161 cgagcgtgcc ggccggaatc agtttcttga gcgcgaaata ttcggtgtcg acaatgtcga
     2221 acgagttggg ctgcgtgatg atgcgcttgg ccacatcgtc ggttgtcacc ggcacgtatt
     2281 gaatgtcgat gcccgtgtct tccttgaatt tcttcgcgat gtcggcgctc tgattcaccg
     2341 ccgtgccgag gtagcgcaag gtgatcttct cctgcgcatg cacgtacgga aacccggcaa
     2401 tgccggccgc cgccaccgcg cccttgatga acgtgcggcg cgacaggccc ttttccttaa
     2461 gatcggatgc ggccgctgcc gggctctcgt acgcttcact cattgctttc tcctcagttg
     2521 acggtcgttt gatgttcgcc tgatctttaa tcaacgcctg tttgtcgttc aggtgcagct
     2581 cggttcgcgc ttctggttca gacgttctct tcgctgccat gtcacgcaat gccgtgcaca
     2641 acggcacgcc cgctcaggcc gccagcggat gcgcgtcctg ctcgttccac cagaccacga
     2701 cgcgcgcgtc gggaccgagc cgcgccgggt cgtagtcgcc gtcgctcacc agagaaatca
     2761 gttcaggcga gccgtcgagc gggtcgagcg tcatgcgtaa atgggtaccc tgatactccg
     2821 cttcgcgtac cgtgcagggc acgccgccga tctgggtcag gccgttctcg cgggttgccg
     2881 tctggaccgg cgtgacgccg tgcggcacca cgcgtagatg atcggcgcgc acggtaaacg
     2941 aacggccatt cgtgttgaag acgttgtgac cgccaagaaa acgcgcgacg aattcagtac
     3001 gcggacggtt gaagacttcg tgcggtgtgc cgctttgttg gatccgcccg tggctcatca
     3061 cgaccaccag atcggcgagc gccatcgctt cttcctgcga atgcgtgacg tgcacaaacg
     3121 tgatgccgag ttctttctgc cagcgcttga gttcagcacg catctgcaca cgcaggaagg
     3181 ggtcgagcgc ggacagcggt tcgtcaagca atagacagcc aggctgattc agcaacgcgc
     3241 gcgcgagcgc cacgcgttgc tgttgaccgc cggacagttg cgccggtttg cgttcggcat
     3301 acgcggacat cgccaccagt tccagcagct cgccggctcg cttgcggcgc acatccttcg
     3361 ctatgccgcg catcttcagg ctgaacgcga cgttatcgag tgcgctcaga tgcgggaaca
     3421 gcgcgtagtt ctgaaacacc atcgccgtgc cgcgggcagc gggttcctcg cgcgtgacgt
     3481 tgcgcccggc gatcagaatg tcaccctcgg tgactgattc atgcccggcg atcatgcgca
     3541 aggtcgaggt cttgccgcat ccagacggcc cgagcaggca gcagtactga ccgccggcaa
     3601 tgcgcagatc gatcgcatcg acggcaagcg agtcgccgta ttgcttcgac acgtgcacca
     3661 gttcgatatc ggcggctgtg gtttcgagca ccattgaagc aggctgaatc atgaagtgga
     3721 tgtcctcgtg aacggcgaag agcggcgcac gcgaaaccac cgacggcgcc taagcgaagc
     3781 catccaaacg caagacatgt gccatccatc gcatcgttac gcttgccgat tgcaaacgcg
     3841 ccggtgtgct taacgtcgac gagttggctc acatattgca aacgatccgc aacgtcgatc
     3901 gtcagcgatc aaagagaatt ttttgcgcaa tcgcggtgcg tcttcggtcc gtcccggggc
     3961 atgatccggc caattcggat cattcggggt gccacgcgtc gcctttcgtc actcactgct
     4021 tctttacatg aacgacacac ggaacatgcc cgcagcagag aatcgcgccc gtcaacccat
     4081 cgcgacccat ggccgcttcg cgtatcgccc gatcacggac agcgacgcgt tccgctggcc
     4141 cggcgatagc gggctcgcgg tctatctcgg cttcaacatc gaacactttg cgttcggcga
     4201 gggactcggc gccgcgctcg gcccgatctc gccgcaaccg gatgtgctga accatagctg
     4261 gcgcgagtac ggcaaccgcg tgggtgcgtg gcgctgcatc gaactgttcg accaactgga
     4321 tatgccggca ggcacgctga tcaatacggc gctctacgat cattgcccgg aactgatcgc
     4381 ggcctgcgtg gcgcgtggcg acgaactgat cggccacggg cacaccaatg cgcatcggca
     4441 aagcgacctc gacgaaaacg gcgaacgtga gctgcttctg cattgccgcg aacgtatcgc
     4501 cgaagcctcc ggcacgccgc cgcagggctg gctctcgcca tggatatccg agtcgcacct
     4561 gacgcccgat ctgctggctg agaccggtta tcg
//
LOCUS       DATASH010000244         1848 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4384965,
            whole genome shotgun sequence.
ACCESSION   DATASH010000244 DATASH010000000
VERSION     DATASH010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4384965"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="VHV99_06820"
     CDS             <1..955
                     /locus_tag="VHV99_06820"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937043.1"
                     /note="This protein belongs to a clade of FMN-dependent
                     monooxygenases, within a broader family of
                     flavin-dependent oxidoreductases, the luciferase-like
                     monooxygenase (LMM) family, some of whose members use
                     coenzyme F420 rather than FMN; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NtaA/DmoA family FMN-dependent monooxygenase"
                     /protein_id="HEX3635190.1"
                     /translation="DSAARAIGIEGQVAHDDRYDLADEYMSLVYKLWEGSWDDEAVRA
                     DRAGGVYADPSKVRVIHHQGPQYKVDAMHLCAPSPQRTPVLYQAGSSTRGRRFAATHA
                     ECVFVNGQKKEAVKEIVGDIRAQALQLGRRGEDVKVFLGATLIVGRTDAEAQAKFEEY
                     RRYVSSEGALAHAAASLGIDFAKYDIDEPIETGKSQAIVSNVEAMTRAAGPQWTRRKL
                     LEQMVLGSRQTPWVGSAGRIAELMMAWSEETGVDGFNLSRTVVPECFEDVIDLLIPVL
                     QARGAYKTAYGDGTYREKLFGRARLPETHTAAQYRSPGARA"
     gene            1077..1580
                     /locus_tag="VHV99_06825"
     CDS             1077..1580
                     /locus_tag="VHV99_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HEX3635191.1"
                     /translation="MVDKAIFRDAMAGLGAAVNIITTDGASGLAGCTASAVCAVTDEP
                     PTLLVCINRASRNNTVIRENGTLCVNVLSADQRDVAMQFATKDVPIEARFASAVWDRL
                     ETGAPVLRGAVSALDCEIASVKEVGTHTVFFCEIKAARTTDDRDGLIYFSRGFHRVGT
                     VAPSVTA"
     gene            1594..>1848
                     /locus_tag="VHV99_06830"
     CDS             1594..>1848
                     /locus_tag="VHV99_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3635192.1"
                     /translation="MRRSSVIRTAGTKPLPEHETQRGKTASLIIVLLAQVAAMGVWFS
                     STTAIAVIKLSHTIAPFEEALLTSAVQLGFVAGTIASALLS"
BASE COUNT          353 a          576 c          608 g          311 t
ORIGIN      
        1 ggacagcgcc gcacgcgcca tcggcatcga agggcaggtc gcgcatgacg atcgctatga
       61 cctggccgac gaatacatgt cgctggtcta caagctttgg gaaggcagtt gggatgacga
      121 agcagtgcgc gccgaccgcg ccggtggcgt gtacgccgac ccgtcgaagg tgcgtgtgat
      181 tcaccatcag ggcccccagt acaaagtgga cgccatgcat ctgtgcgcgc cgtcgccgca
      241 acgcacgccg gtgctctatc aggcgggttc ttccacacgc gggcgccgtt tcgccgcgac
      301 gcacgccgaa tgtgtcttcg tgaacggcca gaaaaaagag gccgtgaaag agattgtcgg
      361 cgacatccgc gcacaagcct tgcaacttgg ccgtcgtggt gaggacgtga aggtattcct
      421 cggcgccacg ctaatcgtcg gacgcaccga tgccgaggcg caggcgaagt tcgaagaata
      481 ccgccgctac gtgagctcgg aaggggcact ggcgcacgcc gccgcgtcgc tcgggatcga
      541 cttcgcgaaa tacgatatcg acgagccgat cgagacgggc aagagccagg cgatcgtctc
      601 gaacgtcgag gcgatgaccc gcgcggccgg cccgcaatgg acgcgccgca agctgctcga
      661 acagatggtg ctcggcagcc gccagacgcc gtgggtcggc tcggccgggc gcatcgccga
      721 actgatgatg gcgtggagcg aagagaccgg ggtggacggc ttcaacctgt cgcgcacggt
      781 ggtgcctgag tgcttcgagg acgtcatcga tttgctcatc ccggttctgc aagcgcgcgg
      841 cgcttacaaa accgcgtatg gcgacggcac ttatcgcgag aagctgttcg gccgcgctcg
      901 cctgccagag acgcacaccg cggcgcagta tcgctcacct ggcgcgcggg cttgatacgc
      961 ggcgagtcgg caataatctg taccgagtca gcaatcgatc agcaatgaat cagcaatggt
     1021 cagcaatgtc aggcgaacgg caccaaccga tgaaatccca gcgagcgggc accaccatgg
     1081 tagacaaagc gattttccgt gacgcgatgg cgggcctggg cgccgccgtc aacatcatca
     1141 cgaccgatgg cgcgagcggg ctcgcgggct gtaccgcgtc ggcggtatgc gccgtcacgg
     1201 acgaaccgcc cacgctgctg gtctgcatca atcgcgcgag ccggaacaac acggtgatcc
     1261 gcgagaacgg caccctgtgc gtgaacgtgc tctcggccga tcagcgcgac gtcgccatgc
     1321 agtttgcgac gaaagacgtg cccatcgaag cccgcttcgc gagcgccgtc tgggatcgtc
     1381 tggaaacggg cgcccccgtc ttgcgtggcg ctgtcagcgc gctcgattgc gaaattgctt
     1441 cagtcaagga agtgggtacg catacggtgt ttttctgcga gatcaaggcg gcgcgcacca
     1501 ccgacgatcg cgacggcctg atctatttca gtcgcggatt tcatcgggtc ggcacggttg
     1561 cgccctcggt cacggcgtga gcacgcatcg ttcatgagac gctcttcagt gatccgcacg
     1621 gccggaacga aaccgttgcc cgagcacgag acgcaacgcg gcaagacggc gtcgctcatc
     1681 atcgtcctgc tcgcgcaggt ggcggcaatg ggtgtctggt tttcgtcgac gaccgcgatt
     1741 gccgtgatca agctctcgca cacgatcgcg ccgttcgagg aagcgttgct gacgagcgcc
     1801 gtgcaactgg gctttgtcgc gggcaccatc gcctcggcct tgctctcg
//
LOCUS       DATASH010000245        10850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1616034,
            whole genome shotgun sequence.
ACCESSION   DATASH010000245 DATASH010000000
VERSION     DATASH010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 10850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10850
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1616034"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VHV99_06835"
     CDS             complement(<1..644)
                     /locus_tag="VHV99_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX3635193.1"
                     /translation="MNQRTLPQHRAAAAAAESRSAVSTSRFSTHALAAGEQFLAWRQR
                     VGHVVDAPPTRDQIAHGFRGAIDRYAVDGMVFTDCSTESMRLERSVARVSTDSRRDYV
                     FQLFVEGEIGHVSGMRKKRSAPASVRGIVAFDLNQPFRAERPECRLLSLFVPSAIVDE
                     ELRDGAAIHGRIVQQGSPLTGLVLDHLTAVAREIPTLDPVAAAQALHAGAQLLVA"
     gene            1547..3451
                     /locus_tag="VHV99_06840"
     CDS             1547..3451
                     /locus_tag="VHV99_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3635194.1"
                     /translation="MKPGKNSLGKRRARKIAVGVGAALIAVLLALGVWQFQQRGARAT
                     PALTGIASQMRHDPSAWTRDEKDASQMLRDIHDANVAAIGVSPNAILVSKRDGTKYFV
                     TDHNATFSHALLLGEPKAGEAASYQLVWLPDADIRTGASRWVPLFGEVRDAISLLLPL
                     LLIGGMVWFMRREMRGGASLLNKAPTLCFDDVIGAGEAKAALADIRGYLSDPKQFSSM
                     GVRAPCGILMVGGPGVGKTRLAQALAGECGANFISITGSYFSAKYYGVGIQKVKHLFE
                     LARKNAPTVIFIDEADGLAKRTDTGGGPVEAESNRIINQLLAEMDGFASNEGVIIVAA
                     TNHPDNLDEALRRPGRFDRTVQVRLPDREDRAKIFRFYAEKLKSKAADIDYDQLARLT
                     TGLSPATVAMVVNQAGLVARKGGETEIAAKHFMEAIKIARIGDVSGAERALSEDERTR
                     IAVHEAGHGLVAALLGTGVLEEVTILPRGGALGVALITKAQDKHLYRETEIRNEIQVL
                     LGGRNAEILTFSEASSGAASDLQEASRISLDMVSKFGFNRDGDLFSLAALPSQYAGLQ
                     MKSAIEHANVLLKELNELCYGLLHTYEPVLRAIADELLERETVPGETVYRLIKEHKSA
                     LKIVHEPAAA"
     gene            complement(3667..3906)
                     /locus_tag="VHV99_06845"
     CDS             complement(3667..3906)
                     /locus_tag="VHV99_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635195.1"
                     /translation="MPNSPNEHENKPDEDPGSPPTEVSKPIGDAVPTPVEPGLDQPLP
                     HKGETPLPPKKADDEPLGETETPPGVPPPGPSDFA"
     gene            complement(4002..4796)
                     /locus_tag="VHV99_06850"
     CDS             complement(4002..4796)
                     /locus_tag="VHV99_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX3635196.1"
                     /translation="MFHFSFANGLLGTSLQALSVSALLGIIAALLLGGMVKGVVSIGV
                     PLVAMPILSQFMPIKEAVLLLSMPIILGNIPQALEGGDMLPTLKRIAAPLIGTVLGNI
                     VGVSVLISLAPHRAQAAAGLLLMVAALLLLLSPRLTLSPAWAKPAGFVLGFGAAMMES
                     IASVPGPLLAMYLIATGATGKAFTKQIAIILVVSIITLVATFSGGAHASWTDLVISAC
                     ASVPVIVGILVVRPLRDRLPPGAFRVLVLVFVLAAAAQMVWKSGVL"
     gene            complement(4988..6178)
                     /locus_tag="VHV99_06855"
     CDS             complement(4988..6178)
                     /locus_tag="VHV99_06855"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013341781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX3635197.1"
                     /translation="MNNRIAGFDGLRAIAVLMVFFQHRLFGDIGEIGHLGVWIFFALS
                     GFLIIGILSAQRGRIETNSSRFGAELKRFLFRRTLRIFPIYYLMLVVMCVLMAFGMAS
                     PELASGMPFHFAYLSNFWIGSVLHYWPGRYSHLWSLAIEEQFYLVVAPLLLMVAARRH
                     RAVCLTIVAVGLASLLAMRAAQWQDITIYTHPLTNFWLLALGGLGGLMIAGTESRLRV
                     VLGHGMTLFVFSVCVAGFCATEPVWGQLSNPLAFTAISALYGASIAALVCSIACCRNA
                     AVIGLLETGWLVNFGRISYGFYLYHNLIPDLTRNHHATAFFRGTVPMWAHVAGIVASF
                     LISLTMAVLSWRLIEAPILRLKTRRTPVTADTASPAAAQTPAVFAASNRRALRDERAA
                     SDAA"
     gene            6766..7872
                     /locus_tag="VHV99_06860"
     CDS             6766..7872
                     /locus_tag="VHV99_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013341780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OpgC domain-containing protein"
                     /protein_id="HEX3635198.1"
                     /translation="MDTRRGRSIEVDFFRGVVLIVIVLDHIPGSALSHLMLHAYALCD
                     SAEVFVFLGGYASAAAYTAVLAGRGEGAAKMRFVRRCWEIYRAYLLTAVLMLLSGAVL
                     ALLHLNQPMVDLTGWPPFAVQPLREAFDIAVLRRQPYLSSVLPMYVIFALCVPFMVPL
                     ARRSSLAALGLSVAIWALARPLAALFSIDDVASWAFNPFAWQLMFVLGMLCRVKPISE
                     RFYATRTARWLTRTAVVAVLAFAIVKLFVLTQPLPGTLKQNLSVDRVINFIVIAWLAA
                     QFVRMGSIAWLAERLPAVVTVGRTGLVCFVGGTLVSLIVDTATPHTFHGFRGTLIGLG
                     GDLVAIGALLMIARGWNDWRGQQSRTAANGAGYG"
     gene            7869..9542
                     /locus_tag="VHV99_06865"
     CDS             7869..9542
                     /locus_tag="VHV99_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HEX3635199.1"
                     /translation="MRAQVRRAGFKLGSRTLVTVVGAALWLMNTSEAFAAPKEASLAI
                     RCAEFAGAVLPPELIALPTAGVQIESAAMVAATAPGNQQGGEFCRITGRIKALKAGTP
                     DIRFDLNLPRRWNGRALQIGGGGYNGVVVSGTGVMPFSPDRAPLAQGYATFGDDSGHV
                     GDASLAVFGLVDEAVTNFGYAHLKKTHDAALALIARAYGRPPQRMYFAGGSTGGREGY
                     TVMQRYPDDYDGVIANSPALNFSGVRLMGVKLGQAEYATPGGFIPPLLLEHVYQRTLA
                     VCDRLDGAPDGIVSDVAGCREQEADLIASLRCAGRAPAHDECLTDAQLSTLLAMRDGL
                     SLPYKLAWDVTGYRGYNVFQGTRLTGLLGLAHQAERLPVPTFFANGYLFAQGDGYLRY
                     FVTRDAAFDSLKFDPQHPGKYRAQLITLSQTIGSMNPDLSRYIARGGKLITLQGLADE
                     VISPNQTIAYYEALIDRFGADRVNGFMRLYMVPGFQHGSGAFIPSVDLLGALDNWVTR
                     GASPETLTATDIAAATNGRSRPLCRYPLFPRYIGKGNVNLAGSFACAEP"
     gene            complement(9656..10069)
                     /locus_tag="VHV99_06870"
     CDS             complement(9656..10069)
                     /locus_tag="VHV99_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635200.1"
                     /translation="MKHITRTIFASALVMALSGGVYAQAGGGAGGGGGAGGGAGGSAG
                     GSNGIGAGGGNAGGAGGTATPGVGTGTMKSPSDSGYGSPGATSTGGGMGTGTGASPNS
                     PSTNRSKGGMNSGTSNGTRSGTGNGMNNGTMQNGQ"
BASE COUNT         2110 a         3351 c         3403 g         1986 t
ORIGIN      
        1 gcgaccagca attgggcgcc ggcatgtaat gcttgcgccg cggcgacggg gtcgagcgtg
       61 ggaatttccc gggcgactgc cgtcaggtgg tccagtacga ggccggtcag cggtgaaccc
      121 tgctggacaa tgcggccgtg aatcgccgcg ccgtcgcgca actcctcgtc cacgatcgcg
      181 gacggtacga acagactcag aagccggcat tcgggccgtt cggcccgaaa cggctggttc
      241 aggtcgaacg cgacgatgcc ccgcaccgaa gccggcgcgc tgcgtttctt acgcatgccg
      301 ctcacgtggc cgatttcacc ttccacgaac agttgaaaca cgtaatcgcg ccgcgagtcg
      361 gtggagacac gcgcgaccga ccgttccagc cgcattgatt ccgtgctgca gtccgtaaat
      421 accatgccgt cgactgcata tcgatcgatc gcgccgcgaa atccgtgggc gatctgatcc
      481 ctggtaggcg gtgcgtcgac cacatggccc acgcgctggc gccaggccag aaattgttcg
      541 ccggctgcga gcgcgtgcgt actgaagcgg ctcgtggaaa cggcggagcg cgattcggct
      601 gcggcggcag cagcccggtg ttgcggcaat gtgcgctggt tcatgaatcc attactgcgg
      661 acgagaggtg ttccggtggg ctagcggtca acagatattt aagccgctga cgtcaaaaac
      721 tgagacggat ggtaaagcct aacaaaaaaa ctgcgatatc gtccgatgat tagagcctcg
      781 acggcgcaga cgaagctgaa gtggcgtgca ggcgcccgag tctgaacgtg tcgaagggaa
      841 gcgctgcgaa actcggggta ggtgcgggag agaagatcgg tgccgtcagg catcggcaat
      901 aaaaaaagaa gaacctgaga gtgcggcgaa atacggacgg ccccacagag gctcgccgat
      961 aagctgcgag accgttgggc gccttgccca tcggacgttt gggggtgttg ctgcaggcgc
     1021 ctccggactt ggccgagcga ccggccgagg atcgccacgc aagtgtcgcc ctcgccgggc
     1081 gctcgctcgg cgatccccct ccgtcacccc acatatcagt tgtcagttgg tggccacccg
     1141 cctgcctgaa tgaacgtcgc ctatattccg cgatttgctg caattttgac cgctttttaa
     1201 tagggctcgg tgaaatggag gtatgcagaa tacgcgttga atatagggct gataaactga
     1261 gcccggagca gcgatccatt gttattaaac acatgaatcg ctccctcaaa tccgacgccg
     1321 caaaccgtcg gatagacggt agaaatgggc tttttctgca atctggaaag agcggccgac
     1381 cggcaaactc gctggtgtca aagctgctaa aagggacgca tggttcagcg tctttctttc
     1441 cgtacctgca tggtgtcgcg tcaaccgatt ccctcccatc aacctctgcc acctgatttt
     1501 gaccagtacc cagggccccc ctttggcccg tttttggaag tgactcatga agccgggaaa
     1561 gaactcgctt gggaaaagac gtgcacgcaa gattgctgtt ggcgtcggcg ccgcactcat
     1621 cgcggtattg ctcgcattgg gcgtctggca gtttcagcaa cgcggtgcgc gggcaacgcc
     1681 ggcgcttacc ggtatcgcga gtcagatgcg ccacgatccc tcggcgtgga cgcgcgacga
     1741 gaaagacgcg tcgcaaatgc tgcgtgacat tcacgacgcc aacgtagccg cgatcggcgt
     1801 gagccccaac gcgattctgg tgtcgaagcg cgacggtacg aaatacttcg tcaccgacca
     1861 taacgcgacg ttctcgcatg cgctgctgct cggtgagccc aaagcgggag aggccgcgtc
     1921 ctatcagctc gtatggctgc ccgatgcgga catccgcact ggcgcgtcgc gctgggttcc
     1981 gctgttcggc gaggtgcgcg acgcgatcag cctgctgttg ccgctgttgt tgatcggcgg
     2041 catggtgtgg ttcatgcgcc gcgagatgag aggcggcgcg tcgctgctca acaaggcacc
     2101 cacgctgtgt ttcgacgacg tgatcggcgc gggtgaagcg aaggcggcac tggcggacat
     2161 ccgcggctat ctgtccgatc cgaagcaatt ctcctcgatg ggcgtgcgcg cgccctgcgg
     2221 catcctgatg gtcggcggcc caggcgtcgg caagacgcgg ctcgcccagg cgctcgccgg
     2281 cgaatgcggc gcgaatttca tttcgatcac ggggagttat ttcagtgcga agtactacgg
     2341 cgtcggcatc cagaaggtca aacatctgtt cgaactcgcg cgcaagaatg cccccacggt
     2401 catcttcatc gacgaggccg acggcctcgc caaacgcacg gataccggcg gtggccccgt
     2461 cgaagccgag agcaatcgca tcatcaacca gttgctggct gaaatggatg gtttcgcgtc
     2521 caatgaaggc gtcatcatcg tcgccgccac caaccatccc gacaacctgg acgaggcgtt
     2581 gcgccggccg ggccgtttcg atcgcacggt gcaggtccgc ttgcccgacc gcgaagaccg
     2641 cgcgaaaatt ttccgcttct atgcggagaa gctgaagtcg aaggccgccg acatcgacta
     2701 tgaccaactc gctcgcctga ccacgggtct ttcgccggca acggttgcga tggtcgtgaa
     2761 tcaggcgggg ctggtcgcgc gcaaaggcgg cgagacggaa atcgccgcga agcatttcat
     2821 ggaggcgatc aagatcgcgc gcatcggcga cgtgagcggc gccgagcggg cactgagcga
     2881 ggacgaacgc acgcgcatcg cggtgcacga agcgggtcac ggtctcgtgg ccgcgctgct
     2941 cggcaccggc gtgctggaag aggtcacgat tctgccgcgt ggcggcgccc tgggtgtcgc
     3001 gctcatcacg aaggcgcagg acaagcacct gtaccgtgaa accgagattc gcaacgaaat
     3061 tcaggtcttg ctcggcggac gtaacgccga aattctcacg ttctccgaag catcgagcgg
     3121 cgctgcgtcg gatttgcagg aagcctcgcg catcagcctc gacatggtgt cgaagttcgg
     3181 ctttaaccgc gacggcgatc tgttcagcct cgcggcgctg ccgtcgcagt atgcaggctt
     3241 gcaaatgaag agcgccatcg agcacgcgaa cgtgctgctc aaggagttga acgaattgtg
     3301 ctatgggctg ctgcatacgt atgagcccgt gctgcgcgcg atcgccgatg aactgctcga
     3361 acgggaaacc gtgcctggcg aaaccgttta ccgattgatc aaggaacaca agagcgcgct
     3421 gaagatcgtc cacgagccgg ctgcggcttg attcgatttg cgttgatttg cgttgtggga
     3481 gcgcgcgtaa acgcgccaac gcgcttcaca acggtccaag ggatcgcgat gggaaattgc
     3541 cataaagggc gctctgacgg aatgatcccc ggagggatcg ccctaagagc ctcgctctaa
     3601 gaagatctcc ccaagggatc gcctcaagag gatcacctca agagtaccgc cccgaaatcg
     3661 ccgacgctat gcgaagtccg acgggccggg cggcggaact ccgggtggcg tctccgtctc
     3721 gcccaaaggt tcgtcgtccg ccttcttcgg gggcagcggc gtctcaccct tgtgcggcaa
     3781 cggctgatcg agacccggtt cgaccggcgt gggaacggcg tcgccaatcg gcttggagac
     3841 ctcggtgggc gggctgccag gatcttcatc cggcttgttt tcgtgttcgt tcggggaatt
     3901 gggcatattt tcctccatgg cttccggttg attgctgtca gccatcacca gcaatgcgcg
     3961 ttccaccgcc cgaattcacc cgtgcgcgca gtgccgagcc ctcataaaac tccggatttc
     4021 caaaccatct gcgcagcggc tgccagaacg aacaccagca ccagcactcg aaacgcgccc
     4081 ggcggcaacc ggtctctcag cggccgcaca acgagtattc ccacgatcac cggcacactc
     4141 gcgcaggccg aaataaccag atcggtccag ctggcatgcg cgccgccgct aaaggtggca
     4201 accagcgtga taatcgaaac caccagaata atcgcgatct gcttcgtaaa ggctttcccc
     4261 gttgcgcccg tggcgatcag atacattgcc agcaacggcc ccggcaccga agcgatgctc
     4321 tccatcatcg ccgcgccgaa tcccaacacg aagcctgccg gtttcgccca cgccggcgac
     4381 aacgtcagac gcggcgaaag cagcaggagc agcgccgcaa ccatcagcaa gaggcccgcg
     4441 gccgcctgcg cgcgatgagg tgctagcgaa atcaacacgc tcacaccgac gatgttgccg
     4501 agcacggtgc cgatcaacgg cgcggcaatg cgcttgagcg tcggcagcat gtcgccgcct
     4561 tccaatgcct gtggaatatt gccgagaatg atcggcatcg acagcagcag cacggcctcc
     4621 ttgatcggca tgaactggct gagaatcggc atggcaacca gcggcacgcc gatactgacc
     4681 acgcccttga ccatcccgcc cagcaacagt gccgcaatga tgccaagcag cgcggaaacg
     4741 ctgagcgcct gcaggctcgt gccaagcagc ccgttggcga aactgaaatg aaacatggtt
     4801 gaagtccgcg agccaggggc gctgaccgga tggaccggag ctcgcgcggg ccgtggcttg
     4861 tttttatcgt tcggcctgct cagtgcccgt gtgtcgcgca agcgcggaag cagccggcta
     4921 agtgaggccg catgataccg cgcccttact cagtctgtat gaaagcagaa cgtaagggcc
     4981 ggttcgctta agcagcatcg gaagcggcgc gttcatctct cagcgcacgc cggttgctgg
     5041 cagcgaacac ggctggcgtt tgcgcagccg ccggactcgc ggtgtccgcc gtcaccggcg
     5101 tacggcgcgt cttcaggcga aggatcggtg cttcgatcag ccgccaggac agcacggcca
     5161 tcgtcagcga aatcaggaac gacgcgacaa tcccggcgac atgtgcccac atcggcacgg
     5221 tcccgcggaa gaacgccgtg gcgtggtggt tgcgggtcag gtccggaatc agattgtgat
     5281 agagataaaa gccatagctg atgcggccaa agttcaccag ccagccggtt tcaagcaggc
     5341 cgatcactgc cgcgttccgg caacacgcaa tcgaacacac cagcgccgcg atagaggcgc
     5401 catacaaggc gctgatcgcc gtgaaggcaa gcgggttgct caactgaccc cacaccggct
     5461 cggtggcgca aaaccccgcc acgcagacgc taaaaacgaa cagcgtcatg ccatgtccga
     5521 gcacgacgcg caaacggctc tctgtcccgg cgatcatcaa gccgccaagc cctcccaatg
     5581 ccagcagcca gaagttggtc agcggatgcg tatagatcgt gatgtcctgc cactgtgccg
     5641 cgcgcatcgc cagcagtgaa gccaggccga cggcgacgat cgtcaggcag accgcgcgat
     5701 gccgccgcgc cgcaaccatc agcaataacg gcgcaaccac gagatagaac tgctcttcga
     5761 ttgcgagact ccagagatgc gaataacgcc ccggccagta atgcagtacg gagccgatcc
     5821 agaagttcga cagataagcg aaatggaacg gcatgccgct cgccagttcg ggactcgcca
     5881 ttccgaacgc catcagcacg cacatcacca ccagcatcag gtagtagatc ggaaagatgc
     5941 gcaacgtacg ccgaaacagg aagcgcttca gctcagcgcc gaaccggctg ctgtttgttt
     6001 cgatgcgccc gcgttgcgcg gacagaatgc cgatgatcag aaagccgctc agcgcaaaga
     6061 agatccatac gccgaggtgg ccgatctcgc cgatgtcgcc gaatagacgg tgttgaaaaa
     6121 agaccatcag gaccgcaatc gcgcgcaatc cgtcgaagcc ggcaatcctg ttgttcatgt
     6181 gcttgtcttc cagtgcgaag gcgacgcgca aagtgccgcc gttcagggcg atggtggaag
     6241 gaccggagcg cattggcgac ggcaaagtca gccgcgtaaa tggaaagcgt cgccaggacg
     6301 ctcggcaagc cgggctgatc gaagatcatc cgggcagcgt gtccggtcca catgagtggc
     6361 cgccgtcgaa gggtggcatc gcgcgaaaac gcgggatgtc cgacgggcat gcatgaatcc
     6421 atcctacgat gaaaaaaagc gaaaaaaatc gcgggccgga attgacgatt tttttccgtg
     6481 acccttaccg acgtgcccgc gctggatgca ttaaaagccg caaaacttgc cgcaaatttt
     6541 gccgcgaagg gtacacgcga cgactcagta ttaccgtacg ccgacagctg aatttcagat
     6601 tgctgccact gcgcatgcat ctacagggcg cttgtcagcg cgtgtggacg aggtgcacgg
     6661 catcggtggg aacgttctat tctcatggag cccctgccgg aaacagtggt gcacgaccgc
     6721 ttgtcacgac gcattgcatg catgcgccaa attgcgagaa cctgaatgga cacccgccgc
     6781 ggacgctcga tcgaagtgga ttttttccgc ggcgtcgtat tgatcgtcat cgtgttggac
     6841 cacattcccg gcagcgcgct gtcgcatctg atgttgcacg cgtatgcctt gtgcgactcg
     6901 gccgaagtgt tcgtgttcct cggtggctat gcgtcggcgg cggcgtatac ggcggtgctc
     6961 gcgggccgcg gcgagggtgc ggcgaagatg cgtttcgtca gacgctgctg ggagatctat
     7021 cgcgcctatc tgctcacggc cgtactgatg ctgttgtccg gcgccgtgct tgcgctattg
     7081 cacctgaacc agccgatggt ggacctgacc ggctggccgc cgttcgcggt tcagccgttg
     7141 cgcgaggcgt tcgatattgc cgtgctgcgg cgccagccgt atctctccag tgtgctgccc
     7201 atgtacgtga tctttgcgct atgcgtaccg ttcatggtgc cgctggcgcg ccgctcgtcg
     7261 ctcgcggcgc tgggtttgag cgtggcgatc tgggcgctcg cccggccgct ggccgcgtta
     7321 ttcagcattg acgacgtggc cagctgggct tttaatccgt ttgcatggca actgatgttc
     7381 gtgctcggca tgctgtgccg ggtgaaaccc atcagcgagc gcttttacgc cacccgcacg
     7441 gcgcgctggt tgacgcgcac cgcggtggtc gcggtgctgg cgttcgccat cgttaaactt
     7501 ttcgtgctca cgcagccgct gcccggcacg cttaaacaga acctttccgt ggaccgcgtg
     7561 atcaacttca tcgtcatcgc ctggctcgcc gcgcagttcg tgcgcatggg cagcatcgcg
     7621 tggctcgcgg agcgcttgcc ggccgtggtg acagtgggcc gtaccgggct cgtgtgtttc
     7681 gtcggcggca ccctggtgtc gttgatcgtc gatacggcga cgccgcatac cttccacggt
     7741 tttcgcggca cgttgatcgg actcggcggc gacctggtcg cgatcggcgc gctgctgatg
     7801 atcgcgcgcg gctggaacga ctggagaggg cagcaatcgc gcacggctgc caatggcgcg
     7861 ggttacggat gagggcgcag gtgcgtcgtg cgggtttcaa attgggttca cgcacgctcg
     7921 tcacggttgt cggcgcagcg ctatggctga tgaacacctc agaggcgttt gccgcgccta
     7981 aggaagcgtc actcgccata cgttgcgcgg aattcgcggg ggcggtgctg ccgcctgaac
     8041 tgatcgcgtt gcccactgcc ggtgtgcaga tcgaaagcgc ggcgatggtg gcagccacgg
     8101 cgcccggcaa tcagcaaggc ggcgaatttt gccgcatcac cgggcgcatc aaggcgctca
     8161 aggccggcac gcccgatatc cgcttcgacc tgaatctgcc gcgccgctgg aacggccgtg
     8221 cgttgcagat cggcggcggc ggctataacg gcgtggtggt gagcggcacg ggcgtgatgc
     8281 cgttctctcc ggatcgcgcg ccgctcgcgc aaggctatgc gactttcggc gacgactcgg
     8341 ggcatgtggg cgatgcatcg ctggcggtgt tcggcctcgt cgatgaagcg gtgacgaact
     8401 tcggctacgc gcatctgaag aaaacgcacg atgccgcgct cgccctcatt gcgcgcgctt
     8461 atggtcggcc gccgcaacgg atgtattttg ccggcggctc gacgggcggg cgcgaaggct
     8521 acacggtgat gcagcgctat cccgacgact acgacggcgt gatcgccaat tcgcccgcgc
     8581 tgaatttttc cggcgtgcgg ctgatgggcg tcaagctcgg tcaggcggaa tacgccacgc
     8641 ccgggggctt cattccaccg ttgttgctcg aacacgtgta tcaacgcacg ctggcggtgt
     8701 gcgatcggct cgacggcgcg ccggacggca ttgtcagcga cgtggccggc tgccgggagc
     8761 aggaagcgga cctcatcgct tcgttgcgct gtgccggccg cgcgcccgcg cacgacgagt
     8821 gccttactga cgcgcaactc tccaccttgc tggcgatgcg tgacgggctg tcgttgccct
     8881 ataagctggc gtgggacgtg accggctatc gcggctacaa cgtctttcag ggcacgcgtc
     8941 tgaccggcct gctcgggctc gcgcatcaag ccgagcgctt gccggtgccg acctttttcg
     9001 ccaacggcta cctgttcgcg cagggcgacg gctacctccg ctatttcgtg acgcgagacg
     9061 cggcgttcga ctcgctcaag ttcgacccgc agcatcccgg caaatatcgc gcgcagctca
     9121 tcacgctttc acagacgatc ggttcgatga atcccgacct ctcgcgttac atcgcgcgcg
     9181 gcggcaagct catcacgctg caaggcctcg ccgacgaagt catcagcccg aaccagacga
     9241 tcgcctacta cgaagcgctg atcgatcgct tcggcgccga ccgggtcaat gggtttatgc
     9301 ggctctatat ggtgccgggt tttcagcacg gcagcggggc gttcatccca tcggtggatc
     9361 tgttgggtgc gctcgacaac tgggtgacgc gtggcgcatc gcccgaaacg ttgactgcca
     9421 cggacatcgc ggcggcgacc aacggacgct cgcgtccgtt gtgccgttac ccgctgtttc
     9481 cacgctacat cggtaagggc aatgtgaatc ttgccggcag tttcgcgtgc gccgaaccat
     9541 gatgccgacg cctgcgcgct ttcatcgaca ttttccagaa gaaaggggcg gcggcacatt
     9601 atccgaatgc cgccgccccg gctgaggcga ctggcctcaa tgcagttgca gggctctact
     9661 gaccgttttg catcgtgccg ttgttcatgc cgttgcccgt gccgctacgc gtaccgttgc
     9721 tcgtgccgct attcatgccg cccttcgaac ggtttgtcga agggctgttg ggcgacgcac
     9781 cggtgcccgt gcccatgccg ccgccggtgc tggttgcgcc cggcgagccg tagcccgaat
     9841 cgctcgggct tttcatcgtg cccgtgccga cccccggtgt cgcggtaccg cctgcgccgc
     9901 cggcattgcc gccgccggcg ccgatgccat tactgccgcc agcactgccg cccgcaccac
     9961 cacctgcgcc gccgccaccg cccgcaccgc cgcctgcttg tgcataaaca ccgcccgaca
    10021 gggccatcac gagagccgaa gcaaaaatcg tcctggtaat gtgtttcatg gtccacctcc
    10081 atagagtcaa ttgacgtcac gcaagcgcat gtcgctcaca tgaatgaact gccgcaagcg
    10141 gtgtgcctca cgctcgccgg agcatcgata tgaagggcag cgacgtgttc ccaggacgta
    10201 gcgccattcc gggttccaat cgagcgctgg acaccgcagc agtcatgggg gaagtcgagc
    10261 atgagacgtt cccgccatcg cacggcaaga cggggtgaaa cgatagtcat ccgaaataat
    10321 gaaggcgcga tgcgcggcaa gggataggaa cgcaaatcag gatctgccaa acgtgttgcg
    10381 cgtgcgaagc aaccggcctc gcagcagtga gaggccgata aggaacgctc gcgtatccgt
    10441 agcgacaagc aattgttacc tgactctatg ccgcgcgatc actgtttcat gtaaaagcag
    10501 ccgattccag gcgcttatcc gtcgttcggc gacgcgaccg ggagcgcttg catacgaatc
    10561 agggcagtag cattgttaag gcgttccgcc ccatgctaga gtccaatgcg ctaaatcggc
    10621 agcatgttcg aacacgagta aaagtacacg gcccggcgcc cgcgccgcct gctagtaaac
    10681 gactggggaa acttgtatgg aaacactcgg cggaacgcgt ctcgcgcaac tattcaatga
    10741 acggcaaagc gagttattgg gcgagtgggt cgctcagcaa tacgcgatcg ccgcgcgccg
    10801 cggtggtgtc accgaagcgc aattgcgcaa ccagttcgtg cagttcgtcg
//
LOCUS       DATASH010000246         5544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2801672,
            whole genome shotgun sequence.
ACCESSION   DATASH010000246 DATASH010000000
VERSION     DATASH010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5544
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2801672"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..642
                     /locus_tag="VHV99_06875"
     CDS             7..642
                     /locus_tag="VHV99_06875"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016355213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HEX3635201.1"
                     /translation="MLKDVRGNGLIQRYTPNERTNHWITAITFVLLALSGLAMFHPAM
                     SWLYAIFGGGQWTRILHPFVGCVMFLSFLILALRFWHHNYLDRADIQWIKQIDDVLNN
                     REDKLPAIGKYNAGQKLLFFTMVICLLLLLASGIVIWRRYFSFYFPIEVIRLAALVHA
                     AAAFVLIVGIVVHIYAALWIKGSIGAMTRGTVTYGWARKHHPNWFKEIIGK"
     gene            768..1763
                     /gene="fdhE"
                     /locus_tag="VHV99_06880"
     CDS             768..1763
                     /gene="fdhE"
                     /locus_tag="VHV99_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HEX3635202.1"
                     /translation="MVQRILEAGDIESLDHTAIPRIRTPERLAVFSARAARLRQLAAL
                     SNPIAGYLRLMAVLADAQQAVIVSFKAKPPGAELIASAQQHSMPLIPALSGVRDPAWH
                     DVLLLLLDNVQAAGPLMPPLEALIARLRILDQATLESQADAIFAQRFEEVDPASAPFI
                     MAALQVVWTDLAADIDKRDVPYLETLGLCPVCGSHPVASMVRVGGAYDNYRYLQCGLC
                     ATEWHMVRSKCSNCDSTKGIAYHAVGSQDTDEATREADSRNAFLKAESCDECHTYRKI
                     GIQSKDYDFEPFADDLASLTLDLLMGAEGYRRASPHPWLWPEQAGSAEESSGESD"
     gene            1781..3211
                     /gene="selA"
                     /locus_tag="VHV99_06885"
     CDS             1781..3211
                     /gene="selA"
                     /locus_tag="VHV99_06885"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HEX3635203.1"
                     /translation="MSDVTGSELRALMARVPSVEKVLASAEFAALLSEFGRTQVLGAL
                     RHALDSWRASAQSGVASVSALDVAQLQVSVETALRRRNEARLRSVFNLTGTVLHTNLG
                     RALLPDEAVQAVMKALTQPANLEFDLGTGKRGDRDDLIDELICELTGAEAATVVNNNA
                     AAVLLTLSALASKKEVIVSRGELVEIGGAFRIPDIMSRAGARLREVGTTNRTHLKDYD
                     EAIGPQTALLMKVHASNYAINGFTKEVGIEAIAPLAHSRGLAVAVDLGSGTLVDLTQW
                     GLPRETTVRETIEAGADLVTFSGDKLLGGPQAGLIVGRRDLIARIKKHPLKRALRVGK
                     LTLAALEPVLQMYRAPEKLAERLTTLRLLTRSSDAIRLTAERVQPALQRMIGERYVVA
                     AEPMFSQIGSGALPVDVLPSHGLVVRTADGKRGGRQLLALEKRLREMARPVIGRIADD
                     ALRLDLRCLEAADEAQLIEQLKGAHA"
     gene            3208..5121
                     /gene="selB"
                     /locus_tag="VHV99_06890"
     CDS             3208..5121
                     /gene="selB"
                     /locus_tag="VHV99_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HEX3635204.1"
                     /translation="MIVGTAGHIDHGKTSLVRALSGVDTDRLKEEKARGISIELGYAY
                     VPLENGDVLGLIDVPGHEKLVHTMTAGASGIDFALLVIAADDGVMPQTREHLAIVELL
                     AIKRGAIALTKIDRVDAARLQQVHEEIAAFLSGTVLRNAPVFDTCATRDGDSGVAALK
                     AHLHAAAAEWRMKRDDGLFRLAVDRVFTLAGQGTIVTGTVVSGRVSVGDTMLLAPKNQ
                     PVRVRSIHAQNRPAETGRAGERCALNLAGIEKSAIDRGDWIVDPRLSEASERIDVMLT
                     LLADVPLTLEHWAPLHVHLGTQHQVAHVALLDGETLGAGQRARVQFVFERPVCALPGD
                     RFVVRNAQADRTVGGGHVLDPFAPSRKRRSAERLAWLDAIQIVLDTGALDSLLARAPH
                     GLSRSLLERLTGMQAAVLEQPSDTRVVELPGNDALLVADASWRALAARLAATLKQYHE
                     RSPDELGPDISRLRRIAAPLADEALWRALVDDAAARGEIVRRGPWLHLPDHAVTLDDA
                     DRALAAALLPVLKEGHFDPPWVRDLANAHGVSEDRVRQLLRKLARQGELFQVVHDLFY
                     HHEVIRELASIAAVEAQKNAGTVAAAPFRDVTGLGRKRAIQLLEFFDRVGYTRFHRGL
                     HLMRTDSRWLDVL"
     gene            5172..5267
                     /locus_tag="VHV99_06895"
     tRNA            5172..5267
                     /locus_tag="VHV99_06895"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5206..5208,aa:Sec,seq:tca)
BASE COUNT          991 a         1633 c         1825 g         1095 t
ORIGIN      
        1 cacggtgtgc tcaaagatgt gaggggcaac ggtctgattc agcgctacac gccgaatgaa
       61 cggacaaatc actggatcac ggcgatcacc ttcgttttgc tggcgctatc aggcctcgcc
      121 atgtttcatc cggcgatgtc gtggctctac gcgatcttcg gcggcgggca gtggacgcgg
      181 attctgcatc ccttcgtggg ttgtgtgatg ttcctgtcgt tcctgattct tgcgctgcgc
      241 ttctggcatc acaactatct ggaccgcgct gacatccaat ggatcaagca gatcgacgac
      301 gtgctcaaca atcgtgagga caagctcccc gcgatcggca agtacaacgc cggccaaaag
      361 ctgctatttt tcacgatggt gatctgcctg ctgctgttgc tggcgagcgg catcgtgatc
      421 tggcggcgct atttctcgtt ctatttcccg atcgaggtca tccgtctcgc ggcgctggtt
      481 catgccgcgg cggcgttcgt gctgatcgtc ggcatcgtcg tgcatattta tgcggcgttg
      541 tggatcaagg gctcgatcgg cgcgatgacg cgcggtaccg tgacctacgg ctgggcgaga
      601 aagcatcatc cgaactggtt caaggaaatc atcggcaagt aggatcggca aagcataggt
      661 aaaaagcgcg gcgcgacttg ccgcgcaccg cactccgagc cgccgcccgt gttcgacgaa
      721 ccgcggcggc tcgtcagttt tcagcgcagt tttcagaggc cgatactgtg gttcaacgca
      781 ttcttgaagc cggcgatatc gagtcgctcg atcacacggc gattccccgc atccgtacgc
      841 cggaacggct ggcggtcttt tcggcgcgcg cggcgcgcct gcgtcaactg gcggcgctca
      901 gcaatccaat tgccggttat ttgcggctga tggccgtgct ggccgatgcg cagcaagccg
      961 tgatcgtgag tttcaaggcg aagccgcccg gcgccgagct gatcgcgagc gcgcaacagc
     1021 actccatgcc gttgattccg gcgctctccg gcgtacgcga tccggcgtgg cacgacgttc
     1081 tgcttctgtt gctcgacaac gtgcaagccg ccggcccgtt gatgccgccg ctcgaagcgc
     1141 tcatcgccag gctgagaatc ctggatcagg ccacgctcga atcccaggcc gatgcgatct
     1201 tcgcgcagcg tttcgaggaa gtcgatccgg cgagcgcgcc gttcatcatg gccgcgctgc
     1261 aagtggtgtg gaccgacctc gctgccgata tcgacaagcg cgacgtgccg tacctcgaga
     1321 cgctcggcct gtgtcccgtc tgcggatcgc atccggtggc cagcatggtg cgggtgggcg
     1381 gcgcgtatga caattaccgc tacctgcaat gcggtctgtg cgcgaccgaa tggcatatgg
     1441 tgcgctcgaa atgttcgaac tgcgactcga ccaaaggcat tgcctatcac gcggtcggct
     1501 cacaagacac cgacgaggcc acccgggaag ccgattcgag gaatgcattc ctgaaggcgg
     1561 agtcgtgcga cgaatgccat acttaccgca agatcggcat tcagtcgaag gactacgatt
     1621 tcgaaccgtt cgcggacgat ctcgccagtc tcacgctgga tctgctgatg ggggcggaag
     1681 gttaccggcg cgcgtcgccg catccgtggc tgtggccgga gcaggccggc agcgcagagg
     1741 agtcttccgg cgagtccgac tgaagaaaga ggattgcctc gtgagcgatg taaccggctc
     1801 tgaattgcgc gcgctgatgg cgcgtgtgcc gtcggtagaa aaggtgctgg cgtcagccga
     1861 atttgcggcg ctgctgagcg agttcggccg cacgcaggtg ttgggcgcct tgcgccacgc
     1921 gctcgacagc tggcgtgcca gcgcgcagtc cggcgttgcg tcggtgagcg cgctcgatgt
     1981 cgcgcaactg caggtatcgg tcgaaacagc gctgcgccgc cgcaacgagg ctcgcttgcg
     2041 cagcgtcttc aatctgaccg gcaccgtgct gcataccaac ctcggccgcg cgctgctgcc
     2101 ggatgaagcg gtgcaagccg tcatgaaagc gctcacgcaa ccggccaatc tcgagttcga
     2161 tctcgggacc ggcaagcgcg gagaccgcga cgatctgatc gacgaactca tttgcgaatt
     2221 aaccggcgcg gaagcggcaa ccgtggtcaa caacaacgcg gcggcggtgt tgctcacgtt
     2281 gtccgcgttg gcctcgaaga aagaagtgat cgtgtcgcgt ggcgaactgg ttgaaatcgg
     2341 cggcgcgttt cgcattcccg acatcatgag ccgcgcgggc gccaggctgc gcgaagtcgg
     2401 caccaccaat cgcacgcatc tgaaggatta cgacgaagcc attggcccgc aaaccgcgtt
     2461 gctgatgaaa gttcacgcga gtaactacgc aatcaacggc ttcacgaaag aagtcggtat
     2521 cgaggcaatc gcaccgcttg cgcattcgcg tggtcttgcc gttgctgtgg atctgggcag
     2581 cggcacgctg gtggatctga cgcaatgggg tttgccgcgt gagacgacgg tgcgcgaaac
     2641 catcgaagcc ggcgccgacc ttgtcacatt tagcggcgac aagctgcttg gcggcccgca
     2701 ggcaggcttg attgtcggcc ggcgcgatct gattgccagg atcaagaagc atccgctcaa
     2761 acgcgcgtta cgtgtcggca aactgacgct ggcggcgctg gaacctgtgc tgcagatgta
     2821 tcgcgcgccg gaaaagctgg cggagcgact gactacgctg cgtttgctca cccgttcgag
     2881 cgacgccatc cggctcaccg cggaacgggt ccaacccgcg ttgcagcgca tgatcggcga
     2941 acgttatgtg gtggcggcgg agcccatgtt cagccagatc ggcagcggcg cgttgcctgt
     3001 ggatgtattg ccgagccatg gcctcgtcgt caggacggcc gatggcaaac gcggcggccg
     3061 gcaattgctc gcgctcgaga agcgcttgcg cgagatggcg cggccggtca tcggccgtat
     3121 tgcggacgat gccttgcgcc tcgatctgcg ctgtctcgaa gccgccgatg aagcgcaatt
     3181 gatcgagcaa ctgaagggcg cccacgcatg atcgtcggta cggcggggca tatcgaccac
     3241 ggcaagacga gtctggtgag agcgctgtcg ggcgtcgata cggatcgtct gaaggaagaa
     3301 aaagcgcgcg gcatttcgat cgaactgggc tatgcgtacg tgccgcttga aaacggcgac
     3361 gtgctcgggt tgatcgacgt gcctggccac gaaaagctcg tccatacgat gaccgcgggc
     3421 gcaagcggga tcgatttcgc gctgctcgtg atcgccgccg atgatggcgt gatgccgcaa
     3481 acgcgcgagc atctcgcgat tgtggaactg ctcgctatca aacgcggcgc catcgcgctg
     3541 accaagatag accgcgttga tgctgcgcgg ctgcagcagg tgcacgaaga gattgcggcg
     3601 tttctgagcg gcaccgtgct gcggaacgca cccgtgttcg acacctgcgc gacgcgtgac
     3661 ggcgattctg gcgtggccgc gttaaaggcg catttgcacg ctgcggcagc agagtggcgc
     3721 atgaagcgcg acgacggcct gttccgactc gccgtcgatc gggttttcac gctcgcgggc
     3781 caaggcacga tcgtgacagg gacggtggtg tcggggcgcg tcagtgtggg cgataccatg
     3841 ctgctcgcgc cgaagaatca gccggtgcgc gtgcgcagca ttcatgcgca gaatcgtccg
     3901 gctgagactg gacgcgcagg cgagcgttgc gcattgaatc ttgccggcat cgagaagagc
     3961 gcgatcgatc gtggcgactg gatcgtcgat ccgcgtttgt cggaagcttc ggaacggatt
     4021 gacgtcatgc tgacgctcct tgccgatgta ccgttgacgc tcgagcattg ggcgccgctg
     4081 catgtgcatc tgggcacgca gcatcaggtc gcgcatgttg cgttgctcga tggcgagaca
     4141 ctcggcgcag gtcaacgggc gcgcgtgcag ttcgtgtttg aacggccggt gtgtgcgctt
     4201 cccggcgacc gctttgtcgt gcgcaacgcg caggctgatc gcacggttgg cggcggtcat
     4261 gtgctggatc cgtttgcgcc ttcgcgcaag cgtcgctcgg ccgagcggct cgcgtggctc
     4321 gacgcgattc agatcgtgct cgacaccggc gcgctggatt cgctgctggc aagggcgccg
     4381 catggcttgt cccgctcgtt gctggaaaga ctgacgggca tgcaggccgc cgtgctggag
     4441 cagccttctg acacgcgcgt cgtggagctt ccaggtaacg atgcattgtt ggtcgcggat
     4501 gcgtcatggc gtgccctggc cgcgcggctg gctgcgacac tcaagcagta ccacgaacgc
     4561 tcgccagacg aactgggccc ggatatatcg cgcttgcgcc ggatcgcagc gccgcttgcc
     4621 gacgaggcgt tgtggcgcgc actagtcgac gatgcggcgg cgcgcggcga gatcgtcaga
     4681 cgcgggccgt ggttgcatct gccggaccat gcggtgacgc tcgacgacgc cgatcgtgcg
     4741 ctcgcggcag cactgctccc cgtgctgaaa gagggacatt tcgatccgcc ctgggtgcgc
     4801 gatctggcaa acgcgcatgg tgtatccgaa gatcgcgtgc ggcaattgct gcgcaagctc
     4861 gcgcggcagg gcgagctttt tcaggtcgtg cacgacctct tttaccacca cgaagtgatt
     4921 cgcgaactgg cttcgatcgc ggcggtcgaa gcacaaaaga atgccggcac ggtcgcagct
     4981 gcgccttttc gcgatgtcac cggactcggg cgcaaacgtg cgatccagct tttggagttc
     5041 ttcgaccggg ttgggtatac tcgcttccac cgtggcctgc atttgatgcg cacggatagt
     5101 cgttggctcg atgtgctttg attttccgca cttgagttgc gacgcgtggt tcacgtagtt
     5161 caatcaccgt aggaaggcat tcgtatccgg tggtgcggct gggcttcaaa cccagttgag
     5221 ggcgtcagac gctctcaggt cggttcgact ccggctgcct tccgccagac atggcttccg
     5281 ggcaattccg atatctttgt agcggtcgtc attaaacttc cgggagcgcc gttcaaatgc
     5341 gccatctcgt gaacgattgt cgcggctgtg aaccccgcgt tattagtgag agttagttgg
     5401 ctgaccgtga tctccatggg cctctaggtt gccttaaagc caccgtctcg gtggcagaaa
     5461 gaaaaaaatt ccgctgcgcg tcgcggcgga gaggagtgat gcctctaagt cggtgaagga
     5521 gcgctctgcc aaacgtattc ggaa
//
LOCUS       DATASH010000247         7047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4779280,
            whole genome shotgun sequence.
ACCESSION   DATASH010000247 DATASH010000000
VERSION     DATASH010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7047
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4779280"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="VHV99_06900"
     CDS             <1..728
                     /locus_tag="VHV99_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX3635205.1"
                     /translation="FERFPCDAVFGLHNWPGLPLGHFAINRGPMMASFDTFEIVVDGK
                     GSHAAMPEEGIDALVCASHVVIALQTIVSRRISPKEACVVSVTQIHGGEAWNVLPDRA
                     VIRGTVRCFDANVQERVRSLLDEIGTLTAQAHGAQATLSYRYGYPATINAPAEADVAL
                     DAARATVGEAMVTLGCKPSMASEDFAFLAQAKPGAYVWMGVDGPTPSAPLHNPHYDFN
                     DDALATGAAYWVNVVKAFGAARG"
     gene            complement(796..1596)
                     /locus_tag="VHV99_06905"
     CDS             complement(796..1596)
                     /locus_tag="VHV99_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008347445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEX3635206.1"
                     /translation="MKVTNRREAMLKAVLSGMNDVGALCEHFGMSEATVRRDLRALAD
                     DRLIVRTYGGAAASVGIHEPEESLDLRRESFREQKDAIAQAAVSHVQDGDTIFLDGGT
                     TTAAMARLLAGRRDIRVVTNNLLAIGLLAASEVPVTLIGGDLRPSSMSTLGPLAQIAL
                     NRLSVDKAFLGADGVVAGRGLCEASAEQAYLKECIIRQAANVFVLVTSHKLDRASQQH
                     WTPLECDWTLVTDAQPGAPELATFLLHSEVTIETASQQARQSLPHGQS"
     gene            1949..2944
                     /locus_tag="VHV99_06910"
     CDS             1949..2944
                     /locus_tag="VHV99_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HEX3635207.1"
                     /translation="MAQLPIKRAIERVPGGMMIVPLLFGAIIATFLPDTPKFFGSFTN
                     ALFTGALPILAVFYVCMGASIDVKATPYLLKKGGALFAAKVGSAIVVGVVLGHFLGEQ
                     PVSSGLFAGMSTLAVVAAMNDTNGGLYMALMGQYGRSEDVGAYTIMSLESGPFLTMVT
                     LGVAGLSAFPWQTLVGSILPLAAGMLLGNLDREMRSFLSRAVPVMIPFFALALGASLD
                     LHKVWQAGLLGIGLGVAVVILTGIPLYLTDRLTGGTGVAGVAAANTAGNGAAVPALIA
                     AANPVYAEAAKSATLLVASCVVVTAILSPILTAAVAKRVGKRADASRAAALEATR"
     gene            2941..4284
                     /locus_tag="VHV99_06915"
     CDS             2941..4284
                     /locus_tag="VHV99_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016347811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HEX3635208.1"
                     /translation="MSCTSILIIADDLSGAADCAIAFAGAGRSTAVTLNASRAMGAAE
                     VIAVDTDTRRMPPAEAAHCTGAAWLAQRGPGRRLYKKIDSTLRGNWAAEVAALQPLAG
                     LAIVATAFPATGRTVRGGRVLVRGVPLENTETWKLEHAAHSADLNAMVEAVGLRTAPL
                     GVDALRGDPTALRDAIAAVASSGAQALIVDAETDADLRALAQVTASMDEALFWVGSGG
                     LARELASLSDLFDVAAQAAQDAPPEPEQERRAILILVGSLSAVSEQQCAMLRERAGVS
                     ELIVPPVVLRAGEPHLEWAGWQARVGAHLSAHTDLLVRIGRDDLFDPAEGALLSAALA
                     SLVAPFFTHLSGLIATGGETARAMLGTAGIDSLQLLSEIEPGVAVGRPHGNPRLTIVT
                     KAGAFGSEHALYGAYLHLRGTPEFAPGEPGSAHPSDASASSPARETNQRHSGKTS"
     gene            4281..5282
                     /gene="pdxA"
                     /locus_tag="VHV99_06920"
     CDS             4281..5282
                     /gene="pdxA"
                     /locus_tag="VHV99_06920"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEX3635209.1"
                     /translation="MSNYLPVIGITMGDAAGVGPEIVVKSLTHESVYQQCRPLVIGDA
                     ARLEQANRIVGGSTKVRRIEKPADALYERGTIDCIDLALIPADLPFGALSALAGDDAY
                     QYIARAVELAKTDEIDAICTAPLNKEALHAGGHKYPGHTEMLAHLTGIEEVSMMLVAP
                     QLRVIHVTTHIGIIDAIRKIEPGLVQRTIERGNATLVKAGIANPRIAVCGINPHAGEN
                     GLFGYGEEEEKIVPAVQVLRERGWDIEGPLPADTLFFRAGRGDFDLVVAMYHDQGHGP
                     VKVLGLEAGVNVTVGLDVIRTSVDHGTAFDIAGKGIADERSLLEALRQGAELATRRA"
     gene            5362..5970
                     /locus_tag="VHV99_06925"
     CDS             5362..5970
                     /locus_tag="VHV99_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEX3635210.1"
                     /translation="MVMHTRRTEDFRARLLEAGVALFAETGYHGTGVKDIVEHAGVPK
                     GSFYNYFESKEAFGAAILDHYAQEQDAEWKQYSLEAASSDPLLALRGIYARIVADYED
                     CDDRYGCLVGNFAGEIAQSSDLCRLAARRSVNEWRVGCADYLRRGQEYGSVRRDLSAD
                     AMADLFWNAWEGSLLRMKLDDSVEPLKACVHSMFELFFKSPQ"
     gene            6068..>7047
                     /locus_tag="VHV99_06930"
     CDS             6068..>7047
                     /locus_tag="VHV99_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX3635211.1"
                     /translation="MQNFTFHNPTRIIFGEGQIAALDAQIPPNARVLITCGGGSIKSN
                     GVLAQVQAALGERAWHVFSGIEPNPTYETLMEAVALAREKNIDFLLAVGGGSVIDGTK
                     FIAAAVKFDGEPWDILAKQAPVRDAMPFGSVLTLPATGSEMNNGSVISYRAKGDKLFF
                     TSDATYPLFSILDPTTTYSLPPRQIANGVVDAFVHIVEQYLTYPVDARVQDRFAEGLL
                     LTLTEVGPQALAEPHNYAVRANLMWTATLALNGLIGAGVPQDWATHMLGHEITALHGL
                     DHAQTLAIVLPAMLSVRRESKRAKLLQYGERVWNITEGSEDERISAAITAT"
BASE COUNT         1203 a         2196 c         2367 g         1281 t
ORIGIN      
        1 tgttcgagcg tttcccgtgt gacgcggtat tcggtctgca caactggcct ggcttgccgc
       61 ttggccattt cgcgatcaac cgcggcccga tgatggcctc gttcgatacc ttcgagattg
      121 tggtggacgg caaaggctcg cacgccgcga tgccggaaga ggggatcgac gcgctggtct
      181 gcgcgagcca tgtcgtgatc gcgctgcaga ctatcgtcag ccggcgcatc tcgccgaagg
      241 aagcatgcgt ggtgagcgtc acgcagattc atggcggcga ggcgtggaat gtgttgcccg
      301 atcgcgccgt gattcgcggc accgtacgct gcttcgatgc caacgtgcag gagcgcgtac
      361 gcagcttgct ggatgaaatc ggcacgctga ccgcgcaggc gcatggcgcg caagcgacac
      421 tgtcttatcg ctacggctat ccggcaacga tcaatgcgcc cgccgaagcc gacgtcgcgc
      481 tcgatgcggc gcgcgcaacc gtcggcgagg cgatggtgac gttgggctgc aagccgtcga
      541 tggcctcgga agacttcgcg ttcctggcgc aggcgaagcc gggtgcgtat gtctggatgg
      601 gcgtcgacgg acccacgccg agcgctccgc ttcacaaccc gcactacgac ttcaacgacg
      661 acgcattggc taccggtgcg gcgtattggg tgaacgtcgt gaaagcgttt ggcgctgcgc
      721 gtgggtgaag cttcgcggct gtccggtggg accgcgtggt ctgtcagggc cgcgcggagc
      781 cacctcctcg aggccctatg actgcccgtg aggcagagac tggcgcgcct gctgcgaggc
      841 ggtttcgatc gtcacttcgc tgtgcagcag gaacgttgcc aattccggcg cgccgggctg
      901 agcatcggtc accagggtcc agtcgcactc gaggggtgtc cagtgctgct ggctcgcgcg
      961 atccagtttg tgcgacgtca ccagcacgaa gacgttggcc gcctgcctga tgatgcattc
     1021 cttcagatag gcctgctcgg cgcttgcctc gcagagccca cggccggcga cgacaccgtc
     1081 cgcgccaaga aaggccttgt cgacggacag gcggttcaac gcgatctgcg cgagcggccc
     1141 gagcgtgctc atgcttgagg gccgcaggtc gccgccgatc agcgtgaccg gcacctcgct
     1201 cgctgcgagc aggccaattg ccagcaggtt gttggtgacc acgcgaatgt cgcggcgccc
     1261 ggccagcagc cgggccatcg cggcggtggt ggtgccgccg tcgagaaaga tggtgtcgcc
     1321 atcctgaacg tggctcaccg ccgcctgcgc gattgcatcc ttttgctcgc ggaagctttc
     1381 gcgccgcaga tcgagcgatt cctcgggttc gtgaatcccg accgaggcgg ccgctccccc
     1441 atacgtgcgc acgatgagcc ggtcgtcggc gagtgcgcgc aggtcgcggc gcacggttgc
     1501 ttcggacatg ccgaaatgct cgcacagggc gccgacgtcg ttcatgccgg acagcacggc
     1561 cttcagcatt gcctcgcggc ggttcgtaac tttcatggtg atagagggta gcgggtcgtc
     1621 cgaatggtgc gcgcaataga caggaaaggc gccaacgggc gtaagtgtaa tcaaaccgcc
     1681 tgctggagca acgctcgcgg cgcggggcgc gccgcgcccg cggcggcgtc gcggcaggcg
     1741 agcgttgtgt gcattttgat cgactcgatc aatctcggct cgcatcccgc ctgaaagcgc
     1801 ttattgcagt gaatgcagtg aacttatcag ctcattacga cttgccgatg agcggattgc
     1861 gcatagtcgt ttcgtcaagt acactcagaa tgatcgaatc agtcaatgta tatgattgca
     1921 tcggtcactt ttaatcagga gacaatgcat ggcccaactt cctatcaagc gcgcgatcga
     1981 acgcgttcca ggcgggatga tgatcgtccc gctgctgttc ggcgcaatca tcgccacctt
     2041 cttgccggac acgccgaagt tcttcggctc gttcacgaat gcgctgttta ccggcgcgtt
     2101 gccgattctc gccgtgttct atgtgtgcat gggggcgtcg atcgatgtca aggcgacgcc
     2161 ttatctgctc aagaagggcg gcgcgctgtt cgcggcgaag gttgggtcgg cgatcgtggt
     2221 gggtgtcgta ttgggccact ttctgggtga gcagccggtt tcgtccggac tgttcgcggg
     2281 aatgtcgacg ctggcggtgg tcgccgcgat gaacgacacg aacggtggcc tgtacatggc
     2341 gttaatgggc caatatggca ggtctgaaga cgtcggtgct tacacgatca tgtcgctcga
     2401 atccggcccg ttcctgacga tggtcacgct cggcgtggcc gggctgtcgg cgtttccgtg
     2461 gcagacgctg gtcggcagca tcctgccgct cgcggccggc atgctgctcg gcaatctgga
     2521 ccgtgagatg cgcagctttc tcagccgcgc tgtgccggtg atgattccgt tcttcgccct
     2581 ggcgctgggc gcgagcctcg atctgcacaa agtatggcaa gccggcttgc tcggcatcgg
     2641 cctcggcgtc gcggtggtga tcctgacggg catccccctg tatctgaccg atcggctgac
     2701 cggcggcacg ggtgtcgcag gcgtggcggc agccaacaca gcaggtaacg gagcggccgt
     2761 gccggcgctg atcgcagcgg ccaacccggt ctatgcggag gccgcgaaga gcgcgacctt
     2821 gctggtcgcc tcgtgcgtcg tcgtgactgc gatcctgtcg ccgatcctga ctgccgccgt
     2881 cgccaagcgg gtcggcaagc gtgccgacgc cagccgcgct gctgcattgg aggcgacgcg
     2941 gtgagctgca cgagcattct gatcatcgcc gacgacctgt ccggcgccgc cgactgcgcg
     3001 attgcgttcg cgggggcggg gcgcagtacg gcggtgacgc tgaacgcgtc acgcgcgatg
     3061 ggcgcggcgg aggtgatcgc ggtggatacc gacacccgcc gcatgccgcc ggccgaagcc
     3121 gcacattgca ccggcgccgc gtggctggcg cagcgcgggc cgggtcggcg tctgtacaag
     3181 aaaatcgatt caaccttgcg cggtaactgg gccgcggaag tcgccgcgct gcagccgctc
     3241 gcgggcctcg ccatcgtcgc cacggccttt cccgccaccg ggcgcaccgt gcgtgggggc
     3301 cgcgtattgg tgcgcggcgt gccgctcgaa aacacggaaa cgtggaagct cgaacacgcc
     3361 gcgcattccg ccgacctcaa cgccatggtc gaggcggtcg gcttgcgcac cgcgcctctg
     3421 ggtgtcgacg cgctgcgcgg cgatcccacg gcattgcgcg acgccattgc cgcggtggct
     3481 agcagcggcg cgcaggcgct catcgtcgat gcggaaacgg atgcggattt gagggcactc
     3541 gcgcaggtga cggcgtcgat ggacgaagcc ctgttctggg tcggctcagg cggtctggct
     3601 cgcgaactgg cgtcgttgag cgacctgttc gatgtcgccg cgcaagcagc acaagacgcg
     3661 ccgccggagc cggagcagga gagacgcgcg atcctgattc tggtgggcag cctgtcggcc
     3721 gtctcggaac agcaatgcgc gatgctgcgc gagcgtgcgg gcgtgagcga gctgatcgtg
     3781 ccgcccgtcg tgctgcgtgc gggcgagcct catctggagt gggccggctg gcaagcgcgc
     3841 gtcggcgcgc atctgagcgc gcacaccgat ctgctggtgc gaatcggccg cgacgatctg
     3901 ttcgatcccg ccgaaggcgc cctcttgtcg gcggcgctcg cctcgctcgt cgcaccgttt
     3961 ttcacgcatc tgagcggctt gatcgcgacc ggcggcgaaa ccgcgcgcgc gatgctcggc
     4021 acagccggca tcgacagcct gcaactgctt tcggaaatcg agccgggcgt ggcggtgggc
     4081 cgcccgcacg gcaatccgcg cctcaccatc gtcaccaaag ccggcgcctt cggcagcgag
     4141 cacgcgctgt atggcgccta ccttcatctg cggggcacac cggagttcgc tcccggcgag
     4201 cccggatcgg ctcacccgag cgacgcatcg gcatcgtcgc cggctcgcga aacgaaccaa
     4261 cgtcattcag gcaaaacatc atgagcaact atcttccagt catcggcatc acgatgggcg
     4321 acgcagcggg cgtgggtccg gaaatcgtcg tcaagagctt gacgcacgag tccgtctacc
     4381 agcagtgccg cccgctcgtc atcggcgatg cggcgcgcct tgagcaggcc aaccggattg
     4441 tcggcggcag cacgaaagtg cgccgcatcg agaagccggc ggatgcgcta tatgaacgcg
     4501 gcaccatcga ctgcatcgac ctcgcgctga ttcccgccga cttgcctttc ggcgcactgt
     4561 cggcgctcgc gggcgacgac gcgtatcagt acatcgcgcg cgccgtcgaa ctcgcgaaga
     4621 ccgacgagat cgacgcgatc tgcacggcgc cgctgaacaa ggaagcgttg cacgccggcg
     4681 gtcacaagta tccgggccac actgaaatgc tcgcgcacct cacgggcatc gaggaggtgt
     4741 cgatgatgct ggtcgcgccg caactgcgcg tgattcacgt gaccacgcac atcggcatta
     4801 tcgatgcgat ccgcaagatc gaaccgggcc tcgtgcagcg cacgatcgag cggggcaatg
     4861 cgacgctggt gaaggcgggc attgccaatc cgcgcatcgc cgtgtgcggc atcaacccgc
     4921 atgccggcga aaacggcctg ttcggttatg gcgaggaaga ggaaaagatc gttccggcgg
     4981 tgcaagtctt gcgtgaacgc ggctgggaca tcgaaggccc gctcccggcc gacacgctgt
     5041 tcttccgtgc gggacgcggc gacttcgatc tggtggtggc gatgtaccac gatcagggcc
     5101 atggtccggt caaggtgctc ggcctcgaag ccggcgtcaa cgtgacggtc ggcctcgatg
     5161 tgatccgcac ctcggtcgat cacggcacgg cgttcgatat cgcgggcaag ggcatcgctg
     5221 acgaacgcag cctgctcgaa gcactgcgtc aaggcgccga actcgcgacg cgccgcgcct
     5281 gatgcggctt gaaatcgatg gatgtgcgcg gtgccgcccg ctcgcgtggc gcgacaccgt
     5341 gcctttaggg ctgtagttgt catggttatg cacacacgac gcactgaaga ctttcgcgca
     5401 aggctgctcg aagcgggtgt cgcgctgttc gccgaaacgg gttatcacgg caccggcgtc
     5461 aaagacatcg tcgagcatgc cggcgtgccg aagggctcgt tctacaacta cttcgagagc
     5521 aaagaggcat tcggcgcggc gatcctggat cactacgcgc aggagcagga cgctgaatgg
     5581 aagcagtatt cgctcgaagc ggcgtcatct gatccgctgc tggcgctgcg cggcatctac
     5641 gcgcgcatcg ttgccgacta cgaagactgt gacgaccgct acggctgtct ggtcggcaac
     5701 tttgcaggcg agatcgcgca gtcgagcgat ctgtgccggc tcgccgcgcg caggtcggtc
     5761 aatgaatggc gtgtcggctg cgccgattat ctgcggcgcg gccaggaata tggctccgtg
     5821 cggcgcgatc tttccgctga tgcaatggct gacctgttct ggaatgcgtg ggaaggcagc
     5881 ctgttgcgca tgaagctgga tgactcggtc gaaccgctga aggcttgcgt gcattcaatg
     5941 ttcgaactgt ttttcaaatc cccgcagtaa cggtcgtagc tgtttgaacg gcgtcgggtt
     6001 tcgatccggg cagccgttta ttccgtttta agacgaccgg tcatttcaat gtaaggaatt
     6061 agattcaatg cagaacttta cgtttcacaa tccgacacga atcattttcg gcgaaggcca
     6121 gattgccgca ctcgacgcgc agatcccgcc taacgcgcgt gtgctgatta catgcggcgg
     6181 cggcagcatc aagagcaacg gtgtgctcgc gcaagtgcag gcggcgctcg gcgaacgcgc
     6241 gtggcacgtg ttcagcggca tcgagccgaa tcccacttac gaaacgctga tggaagcggt
     6301 cgcgctcgcc cgggagaaga acatcgattt tctgctggcg gtgggcggcg gctcggtgat
     6361 cgacggcacc aagttcattg ctgccgcagt gaagttcgac ggcgagccat gggacattct
     6421 ggccaagcag gcccctgtgc gcgacgcgat gccgttcggc agtgtgctga cgctacccgc
     6481 caccggttcg gaaatgaaca atggcagcgt catcagctac cgcgcgaagg gcgacaagct
     6541 gtttttcacg agcgacgcga cctatccgct cttctcgatc ctcgatccga ccacgactta
     6601 ttcgttgccg ccgcggcaga ttgccaacgg cgtcgtcgat gcgttcgttc atatcgtcga
     6661 gcagtatctg acctatccgg tggacgccag ggtgcaggac cgtttcgccg aaggcctgct
     6721 gctcacgctg actgaagtcg gcccgcaagc gcttgccgag ccgcacaact atgcggtgcg
     6781 cgccaacctg atgtggacgg caacgctggc actgaacggc ctgatcggcg ccggcgtgcc
     6841 gcaggactgg gcgacccaca tgctcggcca cgagatcacg gcgctgcacg gactcgatca
     6901 cgcacagacg ctcgccatcg tgctgccggc catgctgagc gtgcgccgcg agtcgaagcg
     6961 cgcgaagctg ctgcagtacg gcgaacgcgt ctggaacatc accgagggca gcgaggacga
     7021 gcgtatcagt gcggcgatca cggcgac
//
LOCUS       DATASH010000248         1768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2802208,
            whole genome shotgun sequence.
ACCESSION   DATASH010000248 DATASH010000000
VERSION     DATASH010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2802208"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1196
                     /locus_tag="VHV99_06935"
     CDS             <1..1196
                     /locus_tag="VHV99_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3635212.1"
                     /translation="RDAMNGHGKLTIEAGNAALDDVYAKRNAEVTPGQYVMLAVTDTG
                     AGMSPEVRERVFEPFFTTKPEGQGTGLGLSMVYGFVKQSGGHVKIYSEQGHGTTIRIY
                     LPRVRQEEDLETNIDAGPAKGGTETILVVEDDEEVRTTVVEMLSDLGYRVLKAKDAQN
                     ALAIVESGVPIDLLFTDVVMPGPLRSTELARKARERLPAIAVLFTSGYTDNAIVHSGR
                     LDEGIELLSKPYTHEALARKVRYVLQTQNPQLDETAALDPRNLQIAPPVMPEGADSFA
                     VDERLRILLVEDDELIRVSTAELLRTFDCDVLEAEGEHDAKQMLSEHAVNVMLTDVGL
                     AGKSGVELALEVCHSRPDLRVIFLTGCDLVLTPDQRKVLPHAILLRKPYDLLDLIDAL
                     KTPLR"
     gene            1315..1626
                     /locus_tag="VHV99_06940"
     CDS             1315..1626
                     /locus_tag="VHV99_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-potential iron-sulfur protein"
                     /protein_id="HEX3635213.1"
                     /translation="MKTSRRHFLTLSVGVSSTLMLSRLAFADTANALSEADPKAQAVG
                     YKEDAAKVDKAKFPGYAAGQTCANCSLFQGKTTDAYGGCTLFGDKQVAARGWCSSYSN
                     M"
     gene            complement(1677..>1768)
                     /locus_tag="VHV99_06945"
     CDS             complement(1677..>1768)
                     /locus_tag="VHV99_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DOPA 4,5-dioxygenase family protein"
                     /protein_id="HEX3635214.1"
                     /translation="PNTGDALRDHRDAAVWIGHSHELVLSALN"
BASE COUNT          359 a          542 c          567 g          300 t
ORIGIN      
        1 cacgcgacgc catgaacggc cacggcaaac tcaccatcga agccggcaac gccgcgctcg
       61 acgacgtgta cgccaagcgc aatgcggaag tcacgccggg ccaatacgtg atgctcgcgg
      121 tcacggacac cggcgcgggg atgtcgccgg aagtgcgtga gcgcgtgttc gaaccgttct
      181 tcacgactaa gccggaagga cagggcacgg gtctgggcct gagcatggtg tatggcttcg
      241 tgaagcagtc cggcggtcat gtgaagatct acagcgagca aggccacggt acgacgattc
      301 gcatctatct gccgcgcgtg cgtcaggaag aagacctcga aacgaatatc gacgccgggc
      361 ccgcgaaggg cggcacggaa acgatcctgg tcgtcgagga cgatgaggaa gtgcgcacca
      421 cggtcgtcga aatgctgtcc gatctcggct atcgcgtgct gaaggcgaaa gacgcgcaaa
      481 acgcgctggc aatcgtcgaa agcggcgtgc cgatcgacct gctgtttacc gatgtggtca
      541 tgccggggcc gctgcgcagc accgaactcg cccgcaaggc gcgcgagcgg ctgccggcca
      601 ttgccgtgct gtttacgtcg ggctatacgg ataacgcgat cgtgcattcc ggccggctcg
      661 acgagggcat cgaattgctc agcaaaccgt atacgcacga agcgctggcg cgcaaagtcc
      721 gctatgtgct gcaaacgcag aatccgcagc tagatgaaac cgccgcgctc gacccgcgaa
      781 atctgcagat cgcgccgccg gtcatgccgg aaggcgccga cagcttcgcc gtggatgagc
      841 ggttgcggat tctgctggtg gaggacgacg agctgattcg tgtgagcacc gcggagctgc
      901 tgcgtacctt cgactgcgac gttctggagg ctgaaggcga gcacgatgcc aagcagatgc
      961 tcagcgaaca cgcggtcaac gtgatgctga ccgacgtggg gctggccggc aagtcaggtg
     1021 tcgaactcgc gctggaggtg tgccattccc ggcccgatct gcgcgtaatc tttctgaccg
     1081 gctgcgatct cgtgctgacg ccggatcaac gcaaggtgtt gccgcatgcc atcctgctgc
     1141 gcaagcctta cgatctgctt gacctgatcg acgcgctgaa aacgccgctg cgatagtggc
     1201 tcggccgccg gagaccttgt gcgtctgcca gcggaaaagt gggcgctcgc gctagaatct
     1261 gcgcagatcg cccaacccgt tggccctgcc ggaattccgc cgatgaccgc aacgataaaa
     1321 acctcacgcc gccattttct gacgctgagt gtgggcgtga gctcaacgct catgctctcc
     1381 cgcctcgcgt tcgccgacac cgcgaacgca ctgagcgaag ccgatccgaa ggcgcaagcc
     1441 gtcggctata aagaggacgc agcaaaggtc gataaagcga aattccccgg ctacgccgcc
     1501 ggacaaacct gtgccaactg ttctctattt caggggaaga ccaccgacgc ttacggtggc
     1561 tgcacgctgt tcggcgacaa gcaggtcgcc gcgcgcggct ggtgcagttc gtattcgaat
     1621 atgtgacggg gcacgcggcc gcgcgtcaaa atccggtgcc cgaaagcgcc ttggccttag
     1681 ttcaaagcgc tcagtaccag ttcatgcgaa tggccgatcc acaccgccgc gtcgcggtgg
     1741 tcgcgcaagg cgtcgccggt attcggat
//
LOCUS       DATASH010000249         9463 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2408042,
            whole genome shotgun sequence.
ACCESSION   DATASH010000249 DATASH010000000
VERSION     DATASH010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 9463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9463
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2408042"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            208..1440
                     /gene="bamC"
                     /locus_tag="VHV99_06950"
     CDS             208..1440
                     /gene="bamC"
                     /locus_tag="VHV99_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HEX3635215.1"
                     /translation="MTDLRLTKRFAVMLMAGGLVAGCGTSSPTKIDYKSDSKSKQVSL
                     AVPPNMIDETSDQRSLPPQGGETSLTTLQQVQAQAPAPNTLTVVPQVNGMHIQRDGTE
                     SWLVIDNKAPDQAWAQIRRFWQEQGFLLVVDQRDKGVMETDWNETHAQINDGLIRNTL
                     SKAMGTSYVSSERNKYRTRLEAAPNGGTYVFISQKGMREAITGTNNDSTQWQAKPNDP
                     GLEQEYLKRLMAALALADSRAKPADMQSAELSPAGAQTAPNAATAGAKSAAAATAAQN
                     VALSAQSPMPDDAANTTSAQLSSTELTLGEPYDRAWLRVGLALDRSNFTVDDRDATRG
                     LYFVRYVDPKDMTSAEQGFWSQVFHGKKEKVAKQYLVNVRAVTPDQTRVAVVDDKGGI
                     DQTPQAKQIMSLMADQLH"
     gene            complement(2634..3560)
                     /locus_tag="VHV99_06955"
     CDS             complement(2634..3560)
                     /locus_tag="VHV99_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX3635216.1"
                     /translation="MNAKNLLQLLILAALWGASFLFIRVGVADFGVAPLMALRVGIGA
                     IFLAVVLVARRPLRQSATILRTRAFPLLVVGILNSAAPFCLFAYAELTLSAGVTSVIN
                     ATTPLWGALVAFLWLRDRLSTLRTLGLVIGFLGVLMLVWDQIATPDGSAATPLSTGLA
                     AAAALGATLLYGIAANYTKRHLTGVDALTVATGTMTGATIVLLPLAVIYWPAAPIAPH
                     AWGAVIALGVACTGVAYMLFFHLIAVAGPARAITVTFVIPIFGILWGALFLSETVSPG
                     MLQGCGVILVGTALATGVIKRLPWVGPRRADT"
     gene            complement(4205..5803)
                     /locus_tag="VHV99_06960"
     CDS             complement(4205..5803)
                     /locus_tag="VHV99_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEX3635217.1"
                     /translation="MFHQLLTPVGNSLLPSFLVAALPIIVVLLLLGWARRPAWQASLA
                     GLIVGLIVAIFVWQFPVGLAVNSVVAGAVFACWPVMWIVFTAILLYNISQRSGRFAAF
                     RMWMIDNLPNDRRVVLVVIGFSFGALLEGISGFGTPVAITSSLLILLGFPTLEALTFT
                     LIFNTAPVAFGALGVPITVLGAVTHLPADSLAKMVGRQLPFFAFLLPFYVIGVYEGFR
                     NMLRVWPVLLVSGASFALTQFVASNYVNYSLTDVLSSMVSLVLTIAFLRVWKPAADPK
                     FAVNIDRVGEVRGKIGGAQGWYPWIIVSVVVIVWTVAKVFLIGDVKVLWPGLDKAVFI
                     TLYNTPYGAIWDFQPLATGTAILVSAIITALVVKLSAREFGAAIVDTWVQTRIAILTV
                     ATIVGLAYLMNYSGLTYTLGLGVASVGPFFPLVSAFLGWVAVFLSGSDTSGNALFGNL
                     QVVAANQLNLNPILMAATNSSGGVMGKMISPQNISTGVATTELKGKEGVVFAKTFKHS
                     ILLTVILGVLVWVQQNFLQGMIPH"
     gene            complement(6218..6730)
                     /locus_tag="VHV99_06965"
     CDS             complement(6218..6730)
                     /locus_tag="VHV99_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635218.1"
                     /translation="MKSPLNTAVSAISQRLPRRFPRISRTGLALTGAVCVLTLSGCYY
                     PYGYYPAGYYPYYATVPAGATQQDVQVGPPDPQAAQQAQQQFSAQQAQNAQQSPPPAY
                     AVAAVPPVYAAPAYPVYYPPPVYPAYYGYPGWYGPSVSIGFGFSSYCCGGHGGWGGHG
                     GWGGGHGHYH"
     gene            complement(7135..9066)
                     /gene="thiC"
                     /locus_tag="VHV99_06970"
     CDS             complement(7135..9066)
                     /gene="thiC"
                     /locus_tag="VHV99_06970"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEX3635219.1"
                     /translation="MNANPKFLSADAHVDAAAVAPLPNSRKVYVTGSRADIRVPMRAV
                     SQADTPDSFGGEKNPPVFVYDTSGPYSDPEAKIDIRAGLPALRQAWIEERGDTEALTG
                     LSSDFSRERAADTATADLRFKGLHRTPRRAVAGKNVSQMHYARRGIITPEMEYIAIRE
                     NQQRAAYLESLKTSGPNGEKLAAMMGRQHPGQAFGASAFGPDGLKEITAEFVREEVAR
                     GRAIIPNNINHPESEPMIIGRNFLVKVNANIGNSAVTSSIGEEVDKMTWAIRWGGDTV
                     MDLSTGKHIHETREWIIRNSPVPIGTVPIYQALEKVNGKAEDLTWEIFRDTLIEQAEQ
                     GVDYFTIHAGVRLQYVPLTAKRMTGIVSRGGSIMAKWCLAHHKESFLYEHFEDICEIM
                     KAYDVAFSLGDGLRPGSIYDANDEAQLGELKTLGELTQIAWKHDVQTMIEGPGHVPMQ
                     LIKENMDLQLEWCDEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWFGTAMLCYVTPK
                     EHLGLPNKDDVKTGIITYKLAAHAADLAKGHPGAQVRDNALSKARFEFRWEDQFNLGL
                     DPDKAREFHDETLPKDSAKVAHFCSMCGPHFCSMKITQDVREFAAQQGVTDDEALKKG
                     MEVKSIEFMKKGAEIYQRQ"
     regulatory      complement(9245..9363)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT         1874 a         2910 c         3044 g         1635 t
ORIGIN      
        1 ggagcctctt ttcgcaccgg gaggctcgct ggatcgtggc gattcagccg cctccacgcc
       61 gatccacacg ctgctgcgtt tggccgaccg cgggccgctc aattcgcgca ccacacccaa
      121 gtttttgcac atgtctcgcc gggatcggta aaatgttggg ttgatccacg gaaacttgcc
      181 gtggcgtagc ggaaggattc cccgaacatg actgatcttc gtcttaccaa gcggtttgca
      241 gtaatgctga tggcaggcgg tctggtagcc ggctgcggca cctcgtcgcc gaccaaaatc
      301 gactacaaaa gcgactcgaa gtcgaagcaa gtgtcactgg ccgtaccgcc gaacatgatc
      361 gatgagacgt ccgatcagcg ttcgttgccc ccgcaaggcg gcgaaacctc gctgaccacg
      421 ctccagcagg tccaggcgca agccccggcc cccaacaccc tgacggtggt gccgcaggtg
      481 aacggcatgc acatccagcg cgacggtacg gaaagctggc tcgtgatcga caacaaggcg
      541 cccgaccagg catgggcgca aatccgccgt ttctggcagg aacagggctt cctgctggtg
      601 gtcgaccagc gcgacaaggg cgtgatggaa accgactgga acgaaacgca cgcacagatc
      661 aacgacggcc tgattcgcaa cacgctgtcc aaggccatgg gcaccagcta cgtgagttct
      721 gagcggaaca agtaccgcac gcgtctcgaa gcggcgccga acggcggcac ctatgtgttc
      781 atcagccaga agggcatgcg cgaggccatc accggcacga acaacgattc gacccagtgg
      841 caagccaagc cgaacgatcc gggtctcgaa caggaatatc tgaagcgctt gatggcagcg
      901 ctggcgctgg ccgattcgcg cgcgaagccg gccgatatgc aaagcgccga gctctcgccg
      961 gcgggtgcgc aaacggcacc gaacgcggct acggcgggtg cgaagtcggc ggcggctgcc
     1021 acggccgcgc aaaacgtggc actgtcggcg caatcgccca tgccagatga cgcggcgaac
     1081 actacgtcgg cacagctttc gtccactgag ctgacgcttg gcgagccgta tgaccgtgcg
     1141 tggttgcgcg tgggcctcgc gctcgaccgc agcaacttca ccgttgacga tcgcgatgcg
     1201 acgcgcggtt tgtacttcgt gcgctacgtc gatccgaagg acatgacatc ggcagagcag
     1261 ggcttctgga gccaggtgtt ccacggtaag aaggaaaagg tcgccaagca gtatctcgtg
     1321 aacgtgcgcg cggtgacgcc ggaccagacc cgtgtggcag tggtggacga caagggtggt
     1381 attgatcaga ccccgcaggc caagcagatc atgagtctga tggccgacca gttgcactga
     1441 acgaagttgt agcaagcagc gaatgctgtc tgaaaacccg cccggcaggc aatccgggcg
     1501 ggttttttta cgcctgtctt cttcctcgcc cgggcacccc cgcatgccat gaaaccgtcg
     1561 tcgtagcgcg cacgtgtttt gcttacgtct agaacaccga tatgaatcgg cgcttgacag
     1621 gagatggcca tgttcgacgt aatcccctat tggatgtggg cggcgagctt gcttgtcctc
     1681 gcgcttgtat gtggcgcggc cttaggtcag cgcgacacgc ggcggccaac tggcttgagg
     1741 gtccggcata cgtcagtgca acgcccgaaa tggccggtgc actgatatgt tcgactgctt
     1801 cagacgcctg aaccgacacg ttgcggccgt tcctccgtgc ggccgcgtgt aacgtgacgt
     1861 tgccccggtg acgtaactcg caaacctcct tccgccaatc ctgtcaatat tcgttcgttg
     1921 aaaaaattac cataacaatc aacgatttgt gattaatttt tcgtcaagtt gcataattgg
     1981 catgcaagct gcttttagag ggtcatggga ccggattcgt aacatccgat gcaagtgcgg
     2041 tgaatttcca cacggcgttc ccagatcgct ccagcaccgg cgaacgaacc ggtgctagcc
     2101 cgatgacggc ctgcgtgcgt ggcgcgcggc ctcggtggcc ccgtcgccta tcgtagggcg
     2161 acggtttcgc ccgcactccc aatggagtgc gggctatttt tcggggtgct cgaaatggac
     2221 ggccatgccg cagacgcggc gagaggtagc cgggctgtcg acgcagcgag aggctgccag
     2281 gcctccgacg ctacgagaga ctgcgggccc tccgacacga cgagaggctg cccggcttcc
     2341 gatgcaacga gaggctgcca ggcctccgac acgacgaaag tttgcccgga cttcgaccaa
     2401 cgcgaagctg ccaggcctcg acgcaacgaa aggctaccct gccttcgacg acgaaaggct
     2461 gccaggcctc cgacacggcg gagagcttgc ccggccgtct acgcagcgag agccacggga
     2521 cagaaaagcg gcgacgccga aggaggcgtc gcgcgaagcc gtgttccgga gtccgcgtgg
     2581 ctctcgataa ggcgagcggc acacggcgcc aaacgcatcg cgccaggcgg cattcaggtg
     2641 tcggcgcggc gcggcccgac ccaaggcaga cgtttgatca cgccggtggc cagcgcggtg
     2701 ccgacgagaa tcacgccgca gccttgcagc atgcccggcg acacggtttc gctgagaaac
     2761 aacgcacccc acagaatccc gaagatcgga atcacaaagg tcaccgtaat cgcgcgtgcc
     2821 ggcccggcca ccgcgatcag atggaagaac agcatgtagg caacgccagt acacgcgaca
     2881 cccagcgcga tcaccgcgcc ccatgcgtgc ggcgcgatcg gcgcagccgg ccagtagatc
     2941 acggcgagcg gcagcagcac gatggtggcg ccagtcatcg tgccggtcgc gacagtcagt
     3001 gcgtcgacgc cggtcaggtg acgctttgtg taattggcgg caatgccata gagcagcgtg
     3061 gcgccgagcg cggcggcagc cgcgagcccg gtggacagcg gcgtggcggc cgagccgtcc
     3121 ggtgtggcga tctggtccca taccagcatc aggacgccga gaaagccgat cacgaggccg
     3181 agcgtgcgca gcgtgctcag gcggtcgcgc agccacagga atgcgacgag cgcgccccac
     3241 agcggcgtgg tcgcattgat cacggacgtc acgcccgccg acagcgtcag ttcggcatag
     3301 gcgaacaggc agaagggcgc agccgaattc aggatgccga ccaccagcag cggaaaagcg
     3361 cgcgtgcgca ggatcgtcgc ggattgacgc agcggccgcc gcgcgaccag gacaacggcg
     3421 aggaagatcg cgccgatgcc gacacgcagc gccatcagcg gcgcgacgcc gaagtcggcc
     3481 acgccgacac ggataaacag aaacgaggcg ccccacaggg cggccaggat cagcaactgc
     3541 agcaggtttt tggcattcat ggtggtggcg ccgcgcgcgg ttgagtcggg acgcctgagc
     3601 atcgtagcag cgcgattgcg cgaaacgttt caggctggaa tgaaacggca agattcggga
     3661 gaggtgaagg acgtctgata gtcgtcatcg taagcgcagc ccgataggcc gcaaaacgag
     3721 caattctcac gctgatgtga gaaaaattgc gatgagatcg gcgtgcggat ggtgaggcgc
     3781 aggcccagta atatcggtgg atgaaagagc ctgaattatt ttgcgacagg tagtcgcgcg
     3841 cgggacgttg acggcggtgc tattggcgcg agcagagcca gccgattacg ctgactcggg
     3901 cgactcgaaa gcaaggcctc cgtgccggct cgggcgagtc gaatacaggc catcaataag
     3961 caacgggcgt gcatccggaa gagcccgccc gccggttcat tcatgcagat gccaactact
     4021 tgatcggacc catcgcccag gtcatcgttt caaggtggac cggcgcgccg atacatacgc
     4081 cgactggccg ctcatgcaga tcctggctga ctgatcggac ccatcgccca agccatcgct
     4141 tcaaggtgga ccaagcgcat gtcgttacgc cgacgaacca cgcacacctc aggtcagcca
     4201 cacctcaatg cgggatcatc ccctgcagga agttctgctg cacccagacc agtacgccga
     4261 gaatcaccgt caacagaatc gagtgcttga aggtcttggc gaacacgacg ccttccttgc
     4321 ccttgagttc ggtggtcgcc acgccggtcg agatgttttg tggcgagatc atcttgccca
     4381 tcacgccgcc cgacgaattg gtcgccgcca tcaggatcgg gttcaggttc aactgattgg
     4441 ccgccaccac ctgcaggtta ccgaacagcg cgttgcccga cgtgtcactg ccggacagaa
     4501 acaccgcgac ccagccgagg aacgccgaca ccagcggaaa gaacggtccg accgacgcga
     4561 cacccagccc gagcgtgtag gtaagccccg agtagttcat cagatacgcg aggccgacga
     4621 tggtcgccac cgtcagaatg gcgatgcgcg tctggaccca cgtgtcgacg atggccgcgc
     4681 cgaattctcg cgcgctcagt ttcaccacga gcgccgtgat gatcgccgac accagaatcg
     4741 ccgtgccggt cgccaaaggc tggaagtccc agatcgcgcc gtacggcgtg ttatacagcg
     4801 tgatgaacac ggccttgtcg agccccggcc acagcacctt gacgtcgccg atcaggaaaa
     4861 ccttcgcgac ggtccacacg atcaccacca ccgagacgat gatccacgga taccagccct
     4921 gggcgccgcc gatcttgccg cgcacctcgc cgacgcggtc gatgttgacc gcgaatttcg
     4981 gatcggcggc gggtttccag acgcgtagaa acgcaatggt gaggaccagc gacaccatcg
     5041 acgacaacac atcggtcagg ctgtagttca cgtagttcga cgcgacgaac tgcgtcagcg
     5101 cgaagctcgc accggacacc agcagcaccg gccatacgcg cagcatgttg cgaaaaccct
     5161 catacacgcc gatcacataa aacggcagca ggaaggcgaa gaacggcaat tggcggccca
     5221 ccatcttggc gagcgaatcg gccggcagat gcgtgaccgc gccgagcacg gtgatcggca
     5281 cgccgagcgc gccgaacgcg accggtgcgg tattgaagat cagcgtgaag gtgagggctt
     5341 cgagggtagg gaagccaagc aggatcagca gcgaactggt aatcgcgacc ggcgtgccga
     5401 agccggaaat cccttcgagc aaggcgccga acgaaaagcc gatcacgacc agcacgacgc
     5461 gccggtcgtt cggcaggttg tcgatcatcc acatgcggaa ggccgcgaag cggcccgagc
     5521 gctgcgagat gttgtagagc aggatcgccg tgaacacgat ccacatcacc ggccagcagg
     5581 cgaagaccgc gccggcaacc accgaattga ccgcgagccc caccggaaac tgccacacga
     5641 agatggccac gatcaggccg acgatcagcc ccgcgagcga agcctgccat gcgggccggc
     5701 gcgcccagcc gagcagcagc aggacgacga tgatcggtag cgcggcaacc aggaatgacg
     5761 gcaatagtga attgccgacg ggggtgagta gctggtggaa catccacgtc tccttcgttg
     5821 ttgttcgaac tcgacgcggg actacgcact gcgggcgcgt cgctctggca ggacggatgg
     5881 gcgccagcgg cgcgtcgaag acgtgccggg tcgcttattt aaacaggtac tgcatgtgtg
     5941 tgacttccca aagcataagg cgcgggcgtc aggcgtcaag aagggaaact acgggcgcgc
     6001 gaagaaaggg cgagagcgaa gcgcggaggc gagcgggccg cgttgtttgc gccaggacgc
     6061 gcgtggcgcc gattcggcgc ggcaaggtac atgccggcgg gctggccggc tttcgggcaa
     6121 cgcgggacgc gcgcgttatg cggcatagac cggtcccgtg cctggcaaat ggcggacacg
     6181 ggatgctcga cgcggtcagc gagaccgggc tgcgagacta gtgatagtgg ccgtggccac
     6241 caccccaccc gccgtggcca ccccagccac cgtggccgcc gcagcaatag ctgctgaaac
     6301 cgaagccgat ggagaccgac ggcccgtacc agccgggata cccgtagtaa gccggataca
     6361 ccggcggcgg atagtagacg ggataagccg gcgccgcata gacggggggc acggccgcaa
     6421 ccgcatatgc gggcggcggg ctctgctggg cgttctgcgc ctgctgcgca gaaaactgct
     6481 gctgggcctg ctgcgccgct tgcggatccg gcggcccgac ctgcacgtcc tgttgtgtcg
     6541 cgcccgcagg gaccgtggcg taatacgggt agtagccggc cggatagtaa ccgtaagggt
     6601 aatagcagcc ggacaatgtc agcacgcaga ccgcgccggt gagcgccagt cccgttcgtg
     6661 aaatacgtgg gaagcggcgc ggcagccgct ggctgatcgc gctgaccgcg gtgttgaggg
     6721 gggacttcat gacgcgctcc tgtttaaatc ggacgccgcc attcggggcg taatggcttg
     6781 agcttacgcc gccgtcgcgc cagtaggatt gcaaaaccgt aacggcttat ttcacctcat
     6841 tgcggatcat tttgcgaagc gaccggcgtg gccgcggtcg ctttcgtcgt acggtcaaac
     6901 gcctgtcaat cagcggcact ttgcgtttga tgttgtcccc gaagcccgta tccagatggg
     6961 tgagcttgtc agcagactaa tgggttcgta atcaaatgtt tcccttcggc gagcatgcgg
     7021 cgtgcccgtg cgtgcggaaa cgcaaaagcc gccagtggcg ggcttcggtg cagcaacgcg
     7081 gtcatgcgga agttcgaatg gcgggcgcgt actgatgcgc ccgcaatccg ccccttactg
     7141 ccgctgataa atttcggcgc ctttcttcat gaactcgatc gacttcactt ccatgccttt
     7201 cttcagcgct tcgtcgtcgg tgacgccttg ttgtgcggcg aattcgcgca cgtcctgggt
     7261 gatcttcatc gagcagaagt gcgggccgca catcgaacag aagtgcgcga ccttggccga
     7321 atccttcggc agcgtctcgt cgtggaattc gcgcgccttg tccgggtcga gaccgagatt
     7381 gaactggtct tcccaacgga attcgaagcg tgccttcgac aacgcattgt cacgcacctg
     7441 cgcgcccgga tgaccctttg ccaggtcggc ggcgtgcgcg gcgagcttgt aggtgatgat
     7501 gcccgtcttg acgtcgtcct tgttcggcaa gccgaggtgc tccttcggcg tgacgtagca
     7561 gagcatcgcc gtgccgaacc agccgatcat tgcagcgcca atgcccgagg tgatgtggtc
     7621 gtagccaggc gcgatgtcgg tggtcaacgg tccgagcgtg tagaagggcg cttcgtcgca
     7681 ccattccagt tgcaggtcca tgttttcctt gatcagttgc atcggcacgt ggccagggcc
     7741 ttcgatcatg gtctgcacgt cgtgtttcca cgcgatctgc gtcagctcgc ccaaggtctt
     7801 cagttcgccg agttgtgctt cgtcgttcgc atcgtagatc gagccgggac gcaggccgtc
     7861 gccgagcgag aacgccacgt catacgcctt catgatttcg cagatgtctt cgaagtgctc
     7921 gtacaggaag ctttccttgt ggtgagccag gcaccacttc gccatgatcg agccgccgcg
     7981 cgagacgata cccgtcatgc gttttgccgt cagcggcacg tattgcagcc gcacgcccgc
     8041 gtggatcgtg aagtagtcga cgccttgctc ggcctgttcg atcagcgtgt cgcggaagat
     8101 ttcccacgtc aggtcttcgg ccttgccgtt gaccttttcc aaagcctggt agatcggcac
     8161 cgtgccgatc ggcaccgggc tgttgcggat gatccactcg cgcgtttcgt ggatgtgctt
     8221 gccggtggag agatccatca ccgtgtcgcc gccccagcgg atcgcccacg tcatcttgtc
     8281 gacttcctcg ccgatggagg aggtcaccgc cgagttgccg atgttcgcat tcaccttcac
     8341 gaggaagtta cgaccgatga tcatcggctc gctttccggg tggttgatgt tgttcgggat
     8401 gatcgcgcgg ccgcgcgcga cttcttcgcg cacgaattcc gccgtgattt ccttcaggcc
     8461 gtcgggaccg aatgcgctcg cgccgaatgc ctggcccggg tgctggcggc ccatcatcgc
     8521 ggcgagtttc tcgccgttcg ggccgctcgt cttcaggctt tccaggtacg cggcgcgctg
     8581 ctggttctcg cgaatcgcga tgtattccat ttccggcgtg atgatgccgc gacgtgcgta
     8641 gtgcatctgc gagacgttct tgccggcgac cgcgcggcgc ggcgtgcggt gcaggccctt
     8701 gaagcgcagg tcggcggttg cggtatcggc ggcgcgttcg cggctgaagt cgctcgacag
     8761 acccgtcaac gcttcagtgt cgccgcgctc ttcgatccag gcttgacgca gcgcgggcag
     8821 gccggcgcgg atgtcgattt tggcttccgg atcggagtac gggcccgacg tatcgtagac
     8881 gaagaccggc ggattcttct cgccgccgaa gctatccggc gtgtcggctt gggagactgc
     8941 gcgcatcggc acgcggatat cggctcggga gccggtcacg tagaccttgc gggaattcgg
     9001 cagcggggcg acagcggcgg cgtcgacgtg ggcgtcggcg gaaaggaatt tcggattggc
     9061 gttcatgcgg atctcctcgg tcgaccggag ctggcgcttt agcgccttac tccggtccca
     9121 tgcaacttgt tgcggtcgac gagagtgggt gccaggcacc gactccagtc ccattcgggg
     9181 ccgctaagca ggagacgaga cggaagttgt cggaaatcgc gagaagtgag gcgataaagc
     9241 tggagtgcga aatccgtacg cttccctgcg ctggcattat ccagatcagg ttcaaagggt
     9301 atttctcacc cacgcaacac ggacctccaa cctccgtgcg acgcaggacc cccgcgttag
     9361 cagcgcacac gatacaccgc tcgggcgctg cttggcaacc cgtcttgaaa aagtcagatg
     9421 tcggagataa cgccggtcag ccgcgcacta ttcttgttgt gag
//
LOCUS       DATASH010000250         1738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4187850,
            whole genome shotgun sequence.
ACCESSION   DATASH010000250 DATASH010000000
VERSION     DATASH010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4187850"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..150
                     /locus_tag="VHV99_06975"
     CDS             <1..150
                     /locus_tag="VHV99_06975"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX3635220.1"
                     /translation="LKLSELTIAADAVNAPRVRVYAADGRLLGVAKQGEGLLAPERLV
                     VTAAT"
     gene            complement(267..>1738)
                     /locus_tag="VHV99_06980"
     CDS             complement(267..>1738)
                     /locus_tag="VHV99_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012492561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX3635221.1"
                     /translation="NVLDTSIANVSIPSISGDLGVSSDQGTWVITSFAVANAISVPLT
                     GWLTERIGQVRLFMASIILFVISSWMCGLAPTLPFLLASRVLQGAVAGPMIPLSQTLL
                     LASYPRAKAPMALSMWAMTTLIAPVAGPILGGWISDNISWPWIFYVNIPVGAIAAVAT
                     WTIFRNRDSVIKKAPIDGVGLGLLILWVGSLQVMLDKGKDLDWFSSTTIVVLALVAVI
                     SLAFFIVWELTAEHPVVDLSLFSRRNFTGGTIALSIGYGLYFGNLVLLPLWLQTDIGY
                     TATEAGLVMAPVGLFAVLLSPITGKVLPRTDPRYIATLSFLIFALCFWMRSRYTTGVD
                     TYSLLLPTLIQGIGMAGFFIPLVSITLSGLPGNRIPAASGLSNFVRIMCGGIGTSIFQ
                     TAWDHRTIMHHAQLAVQANAYNPVFDQSIRQMGAAGFSQSQAYGLFNTMATQQAAQLG
                     VNDLFFVSAGIFVALIVLIWITRPERAGGDAGAAAAAAH"
BASE COUNT          404 a          553 c          524 g          257 t
ORIGIN      
        1 ctgaagctct ccgagttgac catcgccgct gacgcggtga atgcgccgcg cgtgcgcgtg
       61 tacgcagcgg acgggcgtct gcttggcgtc gcgaagcaag gtgagggcct attggcgccg
      121 gaacgactcg tggttacagc cgcgacctag cgtgcaaatt cagcgccgtc acggcagcga
      181 gctgggccaa aatcggcatc gtggccgcca cgaggtcgtt agtatcgccg tggcatcaag
      241 ccggatgggc atcggtgcgc aaaatctcaa tgcgccgccg cggccgccgc gcccgcatca
      301 ccgccggcac gttccggcct ggtaatccag atcagcacaa tcagcgcgac gaaaatcccg
      361 gccgagacaa agaacagatc gttcacaccc aactgcgcgg cctgctgcgt ggccatcgtg
      421 ttgaagaggc cataagcttg cgactgactg aagcccgctg cgcccatctg ccggatcgac
      481 tgatcgaaca ccggattgta cgcattcgcc tgcacggcca attgcgcgtg atgcatgatg
      541 gtgcggtgat cccacgccgt ctggaagatc gacgtaccga taccgccgca catgatccgc
      601 acgaaattcg acagacccga cgccgccgga atccggttac ccggcaaacc cgacagcgtg
      661 atcgacacca gcggaataaa gaacccggcc atgccgatgc cttgaatcag cgtcggcagc
      721 agcagcgaat aagtatcgac gccggtcgta tagcgcgagc gcatccagaa acacagcgcg
      781 aatatgagga acgacaaggt tgcgatatag cgcggatcgg tgcgaggcaa aaccttaccg
      841 gtaatcggcg agagcagcac cgcgaacagg cccacaggcg ccatcaccag ccccgcttcc
      901 gttgcggtgt aaccgatatc ggtttgcagc catagcggca gcagcaccag attgccgaag
      961 tagagaccgt agccgatcga aagcgcgatc gtgccgccag tgaaattgcg ccggctgaag
     1021 agcgacagat cgaccaccgg atgctcagcc gtcaattccc acacgataaa aaacgcgagc
     1081 gaaatcaccg cgaccagcgc cagcacgaca atcgtcgtcg acgaaaacca gtcgagatcc
     1141 ttacctttgt ccagcatcac ctgcaacgag ccgacccaga ggatcagcag gccgagcccg
     1201 acgccgtcga tcggcgcctt cttgatgacc gaatcgcggt tgcggaagat cgtccacgtt
     1261 gccaccgcgg cgatcgcacc gaccggaatg ttgacgtaga aaatccacgg ccacgagatg
     1321 ttgtccgaga tccagccgcc gagaatcggt cccgccaccg gtgcgatcag cgtggtcatc
     1381 gcccacatcg ataaagccat cggcgccttc gcgcgtggat agctcgcgag cagcagcgtt
     1441 tgcgaaagcg gaatcatcgg gccggccacg gcgccttgca gcacgcgcga ggcaagcagg
     1501 aacggcaggg tgggcgcgag gccgcacatc cacgacgaga tcacgaacag aatgatcgaa
     1561 gccatgaaca ggcgcacctg gccgatacgc tcggtgagcc agccggtcag cggcacggag
     1621 atcgcgttcg cgaccgcgaa cgatgtgatc acccacgtgc cctggtcgga cgacacaccg
     1681 aggtcgcccg aaatggacgg gatcgacacg tttgcaatcg acgtgtcgag cacgttca
//
LOCUS       DATASH010000251         3643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4385996,
            whole genome shotgun sequence.
ACCESSION   DATASH010000251 DATASH010000000
VERSION     DATASH010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4385996"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="VHV99_06985"
     CDS             <1..593
                     /locus_tag="VHV99_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004532449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX3635222.1"
                     /translation="RLLQLFAERAPHANLRSVSLSPSHVERGLESGDVDLAVGYFPDL
                     SGNSFFQQRLFTHRFICLMRTGHPLSMAPLTLAQYVASGHAVVRAEGRSQEVLEHYLE
                     KKRIKRRAVLETPHFMSLPFILARTDLLATVPHAIGFAYVSEHASITLVEPPLPLPRF
                     DLRQHWHRKFHNDPRASWLRGVVAELFNDDMDEWPK"
     gene            717..1355
                     /gene="folE"
                     /locus_tag="VHV99_06990"
     CDS             717..1355
                     /gene="folE"
                     /locus_tag="VHV99_06990"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEX3635223.1"
                     /translation="MVSSKAKKSKPTAAAERPSREEAEAAVRVLLRWAGDNPEREGLL
                     DTPSRVVRSYEEFYAGYQVDPREILARTFSEVDGYDEMIVLKDIRFESYCEHHMVPII
                     GRAHVAYLPEHRVVGISKLARLVDAFAKRLQIQEKMTVQIADTLNEVLQPAGVGVILE
                     AAHQCMSTRGVHKAGVTMVTSRMLGTFRTDPSTRREFLSIVGNPGVVAVANT"
     gene            complement(1739..1870)
                     /locus_tag="VHV99_06995"
                     /pseudo
     CDS             complement(1739..1870)
                     /locus_tag="VHV99_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
     gene            2093..3148
                     /locus_tag="VHV99_07000"
     CDS             2093..3148
                     /locus_tag="VHV99_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX3635224.1"
                     /translation="MVAFPSSAVSTWGSAFVFLNVLLTRLGVPIPAVPVLLFAGSAIA
                     AGTLSFWPILFSAVLGALIGDGAWFAAGRIYGRKLIARLKRISPAVDSQVARARSLFE
                     RFGVPLVAISKFVPGLGLITPPLMGTTAVDTRIYAAWDLAGALAWATFWLLGGAAAER
                     QLHLLLDLVKARGGTVIDILLAAALVYLVYRLQQRHRERRRFAQAASATASQATAGGW
                     RRIVPPKVLDARSLASAGEAQQALRPIPGALALDPHSPEQIDGALRAYDMVIYCICPD
                     SATAVEITQRMRLNGYTRIRALRGGLDAWQRRGFPVEPLALAEELAVGGRVPALYSAA
                     RDAVTLRGLAPRRAQSL"
     gene            complement(3403..>3643)
                     /locus_tag="VHV99_07005"
     CDS             complement(3403..>3643)
                     /locus_tag="VHV99_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEX3635225.1"
                     /translation="YLLEFFEPVLKGGRKRALKRLKQIQKRFGKLNDIVASEMLLRAN
                     MALLAGSGDVDAVLLWFRKERKRRMRSAAGLLRKL"
BASE COUNT          657 a         1241 c         1084 g          661 t
ORIGIN      
        1 cgcggctcct gcaactcttc gccgaacgcg cgccccatgc caatctgcgg tccgtgtcgc
       61 tctcgccctc gcacgtggaa cgtggactcg agtcgggcga cgtcgatctc gccgtaggct
      121 atttccccga tctgtcgggc aacagcttct tccagcaacg gctcttcacc catcgattca
      181 tctgcctgat gcgcacgggg catccccttt cgatggcgcc gctcacgctg gctcagtacg
      241 tggcgtcggg ccacgcagtc gtgcgcgccg aaggacgcag ccaggaagtg ctcgaacatt
      301 atctggaaaa aaaacgcatc aagcgccgcg ccgtactcga aacgccgcac ttcatgagtc
      361 tgccgttcat cctcgcgcgc accgatctgc tcgccacggt gccgcacgca atcggcttcg
      421 cgtacgtgtc ggaacatgcc tcgatcacgc tggtcgaacc gccgctgccg ttgccgcgct
      481 tcgatctgcg gcagcactgg catcgcaagt ttcataacga tccgcgggca agctggctgc
      541 gcggcgtcgt cgccgagctc ttcaacgacg atatggacga gtggccgaag tgaactcgca
      601 ccgcggcggc gaaaggggca tcgacacgac gcgccgcaaa acatggaaca atggccgcac
      661 tcattgaacc cgcgcgctcc gtttaccgtc gacgcgcgat cccagaggag cgacggatgg
      721 tcagtagcaa agcaaagaaa agcaaaccca cagccgcagc agaacgcccc agccgcgagg
      781 aagctgaagc agccgtacgc gtgctgctgc gttgggccgg cgacaatccg gaacgcgaag
      841 gcctgctcga tacgccctct cgcgtggtgc gctcttacga ggagttctac gcgggctatc
      901 aggtagaccc acgcgagatc ctcgcccgca ccttctccga ggtggacggc tacgacgaaa
      961 tgatcgtgct gaaagatatc cgcttcgaga gttattgcga acaccacatg gtgccgatca
     1021 tcgggcgggc gcacgttgcc tatctgcctg agcatcgggt ggtcggcatc tcgaagctcg
     1081 cgcgcctcgt cgatgcgttc gccaagcgtc tgcagatcca ggaaaagatg accgtgcaga
     1141 tcgccgatac cttgaacgaa gtgctgcaac cggcaggcgt cggcgtgatt ctcgaagccg
     1201 cgcatcaatg catgtccacc cgcggcgtac ataaagccgg tgtcacgatg gtcacctcgc
     1261 gcatgctggg cacgttccgt accgatccgt cgacgcggcg cgagtttttg tcgatcgtgg
     1321 gtaatccggg tgtggtggcg gtcgccaata cctgaagcct gaaccgcgct tcggtcgcct
     1381 cgtggtgcgg ctgcggcgtc gaaccctggc ttgccgccta tcggttgtga ctcatgccgt
     1441 tgtgtcatgc caccgcgttg tgccttgcgc accgcttatg cctcatggcg gttacgtctc
     1501 atggcgatta cgtcgcccgt cggtgatgcc tcacggcggg ggcgccttac gtccggggta
     1561 tgccttacgt tcggggtatg ccttacgttc ggggtatgcc ttacgttcgg ggtatgcctt
     1621 gcgttcgggg tatgccttgc gttcggggta tgccttacgt ccggggtatg cctcaaaccc
     1681 ggatacgctc atacctgatc tcgcacacac cggttacgct tcacaccggg cttacgcttc
     1741 acgccatgcc acgctccgca cgcacctggc cgaaggtggc cagctgcacc gccccaagca
     1801 cgcatgcaac gccggtaagt tgcgccacgc taaccggctc ccgtaacacg acaatggcca
     1861 acgccaccgc cgcttcacga aagctggcgc gaatccagct tatggtcgaa ttggaagcga
     1921 gcgccgcggc cagacccatc gggtatcctg agcgaagaaa acccgcaccc cggaacgcga
     1981 acgagcaaac cggacgggca agcagataaa cgcttcagga acacactttg tgtcccggca
     2041 aaacacgctc ccgccgtaac gcggcccaac ggcaataaca ggagttacgc aattggtagc
     2101 gttcccgtcg tcggcggtat ccacctgggg cagcgcattc gtatttctca acgtgctgct
     2161 gacacgcctc ggcgtgccga tccccgctgt gcccgtgctc ctcttcgcag gctcggcgat
     2221 cgctgccggc acactgtcgt tttggccaat cctgttctcc gccgtgctcg gcgcgctaat
     2281 cggcgacggt gcatggttcg ccgccggccg catctacggc cgcaaactga tcgcccggct
     2341 caagcgcata tcgccggccg tggattccca agtcgcgcga gcacgctcgc tattcgaacg
     2401 cttcggcgtg ccgcttgtgg cgatctcgaa gttcgtccca ggcctgggac tcatcacccc
     2461 gccgctaatg ggcacgacag ccgtggacac ccgcatctac gccgcgtggg acctcgccgg
     2521 cgccctcgcg tgggccacct tctggctgct cggcggcgcc gcggcggaaa ggcagttgca
     2581 tttgctgctc gacctcgtca aggcacgcgg cggcacagtg atcgacattc tgctagccgc
     2641 ggcgttggtc tatctggtgt accgcctgca acaacgacac cgcgagcgac gccgtttcgc
     2701 gcaagccgcc tccgctacag cgtcacaggc gacagccggc ggatggcgcc gcatcgtgcc
     2761 tcctaaggtt ctggatgccc gctcgctcgc ctcggctggc gaggcgcagc aggcgctgcg
     2821 ccctattccc ggcgctctgg cgctcgaccc gcattcgccg gaacagatcg acggcgcgct
     2881 gcgcgcctat gacatggtga tttactgcat ttgccccgat agcgcgacag ccgtcgagat
     2941 cacccaacgg atgcgcctga acggttacac gcggattcgc gcactgagag gcggcctcga
     3001 tgcctggcaa aggcgggggt ttcccgtcga gccgctcgcg ctcgccgagg aattggccgt
     3061 cggaggacgt gtgccggcac tatacagtgc ggcacgcgac gcggtcacct tgcgcggcct
     3121 cgcccctcgg cgggctcaaa gcttgtgagc ggcgcgcctg gccggggcgc ttgaccgcgt
     3181 gcctatcgac cacccaagcc ggatggttcc agcgcgcacg ccgtacacgc caaacggttt
     3241 cagggcgcct accgtcgcca cacaccaaac aggtccagcc cgccgaccgc cagcacgagt
     3301 caaacggatc cgccgcgccg cgcccacaat cacgaatccc gcgagatcgc ccatgtcgtg
     3361 attcggcccg ggttactcga acgagccgca ccgcgccgcg catcacagtt tgcgcagcaa
     3421 tccagccgcc gaacgcatcc gccgtttgcg ttcctttctg aaccagagaa gcaccgcatc
     3481 gacgtcgcca gacccggcca gcagagccat gttggcgcgt agcagcattt cgcttgcgac
     3541 aatatcgttc agcttaccga aacgtttctg aatctgcttc aaacgcttca gggcacgttt
     3601 tcggcccccc ttcagaacgg gctcgaaaaa ctcgagcagg tag
//
LOCUS       DATASH010000252        16499 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1418656,
            whole genome shotgun sequence.
ACCESSION   DATASH010000252 DATASH010000000
VERSION     DATASH010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 16499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16499
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1418656"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VHV99_07010"
     CDS             <1..282
                     /locus_tag="VHV99_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635226.1"
                     /translation="EPEGSGARRASSAHTYRLLIFKDRVRIHYRNRTARCWYQASTPQ
                     PPGTASFCVAASAAEKRDYGERSAGRQPPFVNFLLTKCDLTPTHAPHYM"
     gene            671..2674
                     /gene="rpoD"
                     /locus_tag="VHV99_07015"
     CDS             671..2674
                     /gene="rpoD"
                     /locus_tag="VHV99_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013341864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEX3635227.1"
                     /translation="MNPFDREDSQHETAGYATKAPAGRTAKGKGAGKAIPVAAELPPQ
                     QAEERQRQMRALIQLGKEQGYLTHAQINDHLPDNFTQTAAIDSIVSAFNDMGVQVYEQ
                     APDAETLLLNSNAPAVVSDDQADEETEVALSTVDSEFGRTTDPVRMYMREMGATELLT
                     RAGEIAIAKRIEDGLHEMVQAIAACPLAISAILASAQQVADGELRIDELVDGLNEDNV
                     AAEDAGAGADAVADIDADAADDDSEDEDEDDDAGPADSSAADEARLKQLTSDCLEIFA
                     QVGILSDQMNASHTRGDVSSKAYQRAREEIQQHLAKIRFTARTIDRLCGDVQSQVAQV
                     RAIERNILQIVVTKSGMPREQFIESFKGHEADLGWTERAARGSSSFGAALERHLPAIQ
                     SEQQKLIDIEATVSLPLKHLKEINRQMVAAESKMRKAKSEMIEANLRLVISIAKKYVN
                     RGMLFLDLIQEGNIGLMKAVDKFEYRRGWKFSTYATWWVRQAVTRSLADQARTIRVPV
                     HMIETINKLNRISREILQQTGQEAHPSVLAERMELSEEKVRGILKIAKQPVSMETPVG
                     DDGDATLGDMIEDSAASSPADAAMQADLRAAIDDALDSLSPREAKVLRMRYGINTKSD
                     HTLEEVGKQFDVTRERIRQIESKAMRKLMHPSRADRLKSFLDR"
     gene            3250..3726
                     /locus_tag="VHV99_07020"
     CDS             3250..3726
                     /locus_tag="VHV99_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX3635228.1"
                     /translation="MTTLDPQGLASAGTQGANIVGTGIGDGPGPDVMAASTLDGNKVM
                     SSDGEHVGKISDIMLDVRGGRVAYAVLSSGGFLGMGDTLHAIPWSALTLDTDEKCFVL
                     DAAAEHIKNAPGFDKDNWPSMADTEWGTSVHEYYNRPPYWSATRDVVEGRGLDSDL"
     gene            complement(3836..4318)
                     /locus_tag="VHV99_07025"
     CDS             complement(3836..4318)
                     /locus_tag="VHV99_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007582345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX3635229.1"
                     /translation="MVRRLRAWCEQRVVEKLFEAFKKAGDDDLEAKAALAQRACEELT
                     MHAIIEEELLYPAAQEALPDDDKLDVDEAYVEHFLVKTLIAQFETLKAGDKGFDATFK
                     VMSELVQHHVEEEEEALFPALRRTKCDLVGLGKKIAVRKQALQSKLDAVGSRSVGDKT
                     "
     gene            complement(4291..4377)
                     /locus_tag="VHV99_07030"
                     /pseudo
     CDS             complement(4291..4377)
                     /locus_tag="VHV99_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855759.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2300 domain-containing protein"
     gene            complement(4524..5696)
                     /locus_tag="VHV99_07035"
     CDS             complement(4524..5696)
                     /locus_tag="VHV99_07035"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX3635230.1"
                     /translation="MSSKVVIVGGGVIGSSIAYFLRLSDPTVSVTVIERDPTYARSSS
                     ALSAASIRQQFSTPLSIQMSLFGIEFLRSIGERLELDGAKPSIDLHEGGYLFLATSAG
                     ETTLRENHALQKSLGADISLLDKAALRARFPWLNTEDLVAGAYGESGEGWFDGYGLVQ
                     ALRKKAQALGACYVAADVTALHRDGNRVTQVQSANGERYACDVVVNAAGAWSRKVAQM
                     AGIDLPVFARRRSIFNVTSAARLDHCPLLIDPSGVYFRPEGQSFICGTSPSADNDPDD
                     LPLDEVDHALFDDVIWPTLAHRVPQFEALRVQHCWSGYYEYNVLDQNAIIGYHPHVDN
                     CIFANGFSGHGLQQGPATGRGISELILQGRYTTLDLGSLSFTRVLENRPIVEKNVV"
     gene            complement(5843..7342)
                     /locus_tag="VHV99_07040"
     CDS             complement(5843..7342)
                     /locus_tag="VHV99_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX3635231.1"
                     /translation="MDASTILADLGIAYAAQAGDIAVHSPITGELIGRVASNTVAEVD
                     AALTNAKEAYAAWRNVPAPRRGELVRLLGNRLRDKKQALGGIITLETGKILQEGMGEV
                     QEMIDICDFAVGLSRQLYGLTIASERPGHRMAETWHPMGTCVVISAFNFPAAVWSWNA
                     ALALVCGNAVIWKPSEKTPLTALAVNQILREALKEFGDAPAGLSALINGGRDVGAKLV
                     ADPRASIVSATGSTEMGRTVGVEVAKRFGRSLLELGGNNAGIVAQTADHELAMRGILF
                     SAVGTAGQRCTSLRRLFVHESVYDKTIERLKQLYSKVPIGNPLEKGTLMGPLIDKQAY
                     ARMQEALQQATAEGGKVFGGERVEVKGHEGGYYVRPAIVEMPSQTAVVLKETFAPILY
                     VLRYSDFADAVEANNAAVHGLSSCVFTTDLREAERFLSDSGSDCGIANVNIGPSGAEI
                     GGAFGGEKETGGGRESGSDAWKAYMRRATNTVNYSSALPLAQGIDFNIG"
     gene            complement(7636..7764)
                     /locus_tag="VHV99_07045"
                     /pseudo
     CDS             complement(7636..7764)
                     /locus_tag="VHV99_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176143.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            8355..9524
                     /locus_tag="VHV99_07050"
     CDS             8355..9524
                     /locus_tag="VHV99_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX3635232.1"
                     /translation="MKKSLLAFAALSTFAGVANAQSNVTLYGIIDEGVNVVTNSGGQH
                     LYNLSSGELQGSRWGVRGTEDLGGGLKAIFDLENGFDINSGKLGQGGLMFGRQAFVGL
                     SSRLGTVTLGRQYDSIVDYVGPLEVGDQWGGYIAAHPGDLDNFNNAYRTNNTVKYTSA
                     NYNGLTFGGTYSFGGVAGDFTSNQIWSLGAGYNNGPLVLGAAYLNARTPAASGGLFNN
                     GGAVAAASAAVTTSIYSGFASANTYQVIGAGGAYTFGAATIGATYSNIRFDNLGASSA
                     SPYKGQSVSFNNAEVNFKYQLTPAFLVGVAYDYTRGAQIDGASRAQYHQAAVGVDYFL
                     SKRTDVYVTGVYQHALGETLNAAGKVVAATANIDQLSPSSNQNQFTGRIGIRTKF"
     gene            9569..10321
                     /locus_tag="VHV99_07055"
     CDS             9569..10321
                     /locus_tag="VHV99_07055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3635233.1"
                     /translation="MRTSSLLHPVMRIAFIPEHGTLDRGSEQCLPSSADDHREFKTAS
                     SLFRSLKESRWNLVVAGWDLPDVKGDDLLRWMRATFSDSPPVVFLTSHDAAADIVNSL
                     NQGAVDYILAPVEPLVLRARLGAILRRLSSAPDGLVREGEEGRYQDIPPTVRFGRYVF
                     HQRHHYVDIQGEKVMLRPKEYALAMLLFENIGKTLSRESIHMKLWETMDNFPTRTLDT
                     HLSRLRAKLKLVGGNGFDISQIRGVGYRLESC"
     gene            complement(10412..10846)
                     /gene="tssE"
                     /locus_tag="VHV99_07060"
     CDS             complement(10412..10846)
                     /gene="tssE"
                     /locus_tag="VHV99_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011301370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HEX3635234.1"
                     /translation="MTRGGPGLFEAVTGHFANGSAVDDFDPATQTFFSVQDNIQRILN
                     SRRNGLVHLPDYGLDDLSEIYRHLPSSAHKLKGAIETTLLKYEPRLESIDIDIEDTEP
                     GMLLSFTMKCRLCQAGLVRFGTHFTLDGQTRLTMLKGDRDRD"
     gene            complement(10843..13614)
                     /gene="tssH"
                     /locus_tag="VHV99_07065"
     CDS             complement(10843..13614)
                     /gene="tssH"
                     /locus_tag="VHV99_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HEX3635235.1"
                     /translation="MNTRDLSPLLRRLNDHCARALAEAASLCETRAHRDVEIEHWLIK
                     LLELGEGDLVAIVRHYELDADGIWNGLLSAIDRLPHALRGKPGLSHRLGQWLEAAWMC
                     ASLEAGATSIRSAHLLAALADAPHLLRAPDAWPLLSVSAAQIGRLMPALDRISVEAAS
                     VSVQPDGDLDTARHSMGGVPGNGMAGSAMHETASHGAFGDQPSWSAARARATLPGQSA
                     SDALQRFTIDITQKARDGRIDPVFGRDVEIRQMVDILARRRKNNPILVGEPGVGKTAL
                     VEGLALKIVDGDVPPVLRDVSVLTLDLGLLQGGAGVKGEFEQRLKNVIEAVQRSPTPI
                     LLFIDEAHTLIGAGNTAGGADAANLLKPALARGELRTIAATTWSEYKQYFERDAALDR
                     RFQMVKVDEPDNDSACLMLRGLKARYAKHHGVHITDAAVAAAVDLSRRYLTGRQLPDK
                     AVDLLDTAAARVRMSSDATPVAIAARHAEQAALLVEHGALVADHAATSVEVGERLLAI
                     DLRLAHLKVEVDALESTFAEQKDAVATLQRLREQWHAAADESVRRDLQQSIRVVHEVL
                     AGHGADALIQAEVDEAAVARVIADWTGVPVGSLLEDELATLLALETRLGRVVVGQENA
                     LAALGKSLRAAKAGLKSKEAPLGVFLLVGPSGVGKTETARALADLMFGGERALITINL
                     SEYKEAHTVSQLKGAPPGYVGYGVGGVLTEAVRQRPYSVVLLDEVEKAHRDVLNLFYQ
                     VFDRGLMRDGEGRVIDFRNTVILMTSNLGSEQIMAATDAVAEAGGDITTSMLMETIRP
                     LLVEHFQPALLARFQTVVYRPLSAAALTSIVRMKLDKVAQRVERRFGIPLIGDDSLVR
                     ELVRACQLPDSGARNVDSLLDQQILPVLSRELLVRVAAQQLSRGITLSFTEESGIAVE
                     FDSMIEAEA"
     gene            complement(13733..14044)
                     /gene="tssD"
                     /locus_tag="VHV99_07070"
                     /pseudo
     CDS             complement(13733..14044)
                     /gene="tssD"
                     /locus_tag="VHV99_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014070071.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
     gene            14049..15056
                     /gene="tssF"
                     /locus_tag="VHV99_07075"
     CDS             14049..15056
                     /gene="tssF"
                     /locus_tag="VHV99_07075"
                     /inference="COORDINATES: protein motif:HMM:NF017733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HEX3635236.1"
                     /translation="MFVDLIGLDMPAIPLAAEYFDVEVVLAQPYPDDARFSADNVRLY
                     CTPIVNLFALEADPITASQHETEYRVHAREQRGDHVDIYSVESVRGFEAGTGRRFEYA
                     PFTAFRHRGGMLRHEMPERYFHTRTRRGPSGRFDTWIVLGGHAWEQQATLTKETLSLS
                     VTGTNGMVPRKGLRDAGINRMRGGFTNIGAVRNLTAPTLPVYPPTGDRFQWRVLSHLA
                     PNYLSLLNAEILRGSLALYDWTQGELNKRRIDAITDVQHRLLQKLVKGGLQRGVEIEV
                     TLDSAAFAGDGDIELFGGMLNRFLALYATLNLFTKLVIVSQPTGRRMEWPNTKGEGAP
                     F"
     gene            15158..16162
                     /gene="tssG"
                     /locus_tag="VHV99_07080"
     CDS             15158..16162
                     /gene="tssG"
                     /locus_tag="VHV99_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018418254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HEX3635237.1"
                     /translation="MNFFRFCELIELAAPDRPPIGTTDSPASEPVRFRSRARLGFPNR
                     EIDAVEYDLDHPAMPSVVRTTFLGLYGVDARMPSYFVDEVAQNREGAESMSAFLDIFH
                     HRVVTQYYRVWRKYRYPAGFRNDGTDEISRYLLSFAGLGIASPEAAAAGSQGLPSGLG
                     TRKLLSMLGLASQKTRTAEGLVGVLQHAVPDARITVEEFYPVWISAADYPRTPLGEAC
                     LLGRGFFDRANAVRIVITPQTRESVLGLMPGRANHSEVMALLRFYLGYEARARLEMHV
                     APELMPAQALNSNQVSLGYTTQLPRPRMEDPPAPVTRVQLGTWTGSSDRHAARQHSMH
                     "
     gene            16230..>16499
                     /gene="tssJ"
                     /locus_tag="VHV99_07085"
     CDS             16230..>16499
                     /gene="tssJ"
                     /locus_tag="VHV99_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HEX3635238.1"
                     /translation="MMRILRIGATALIILAIAGCGVGQAVKDGTVDAAKWAFTTQVKT
                     MNMDLVSRSSLNANGAGQSLSTVVRIYQLKTPQAFQQLDYTQLQTN"
BASE COUNT         3351 a         5039 c         4892 g         3217 t
ORIGIN      
        1 gagcccgaag gttccggcgc gcgtcgcgca tcaagcgccc acacttatcg gctgttaatt
       61 tttaaagatc gagtccgcat tcactaccgg aaccgcaccg cgcgctgctg gtatcaagcc
      121 tcaacaccac aaccacccgg caccgcttcg ttctgcgtcg ctgcatcagc agcagagaaa
      181 cgagattatg gagaacggtc ggcgggtcgt caaccccctt ttgtgaattt tcttttgacc
      241 aagtgcgatt tgacgccgac acatgcgccc cactatatgt agcaagatcg acggaaactc
      301 atccggccgt gcgggagtgg ccgacatagc ctatagtgaa gagcctgcat acgcgcacga
      361 cgagcagttg aagtgctgcc ttctgccccc acctgaaccg acggatgaca ggcgacgaag
      421 ggaccctgcg acaagagcgc atgacaaatt ggtgtgctca taatgcgcgc gaaggcgcaa
      481 gctcgtatat aatgcgagcc gcgcgaattt cgcacatttc tatcggcccg cttcgcgcgg
      541 gctcatcttt tttgctcccc agagcacagc caaacaaccg cgttgagtca ctcaggtgat
      601 gttgagccat gctcgaaacg cctgacggta gcgtcttgcg tctcatagcg aagcctctag
      661 aggagaatac gtgaaccctt ttgatcgcga agattcgcaa cacgaaactg ccggatacgc
      721 caccaaagcg cccgctggcc gtacagccaa gggtaaaggc gctggcaaag cgatccccgt
      781 agcggccgaa ctgccgccgc aacaggccga agagcgccag cgccagatgc gcgcgctgat
      841 ccagctgggc aaggagcaag gctacctgac ccacgcgcag atcaacgacc acctgcccga
      901 caacttcacg cagacagcgg cgatcgacag catcgtcagc gccttcaacg acatgggcgt
      961 gcaggtctac gagcaggcgc cggatgcgga aaccctgctg ctcaactcga acgcccccgc
     1021 cgtggtgtcg gacgatcagg cagacgagga aaccgaagtc gcattgtcga cggtcgattc
     1081 ggaattcggc cgtacaaccg accccgtgcg catgtatatg cgtgaaatgg gcgcaaccga
     1141 actgctgacc cgcgcgggcg aaatcgcgat tgcgaagcgc atcgaagacg gtcttcacga
     1201 aatggtgcag gcgattgctg cgtgcccgct cgcgatctcc gccattctgg cgagcgccca
     1261 acaggtcgct gacggcgaat tgcgcattga cgaactggtc gacggtctga acgaagacaa
     1321 cgtcgcggcg gaagacgccg gcgccggtgc agacgccgta gcggacatcg acgccgacgc
     1381 ggccgacgac gacagcgaag acgaagacga agacgacgac gccggtccgg cagactcctc
     1441 ggcggcggac gaagcgcgcc tgaagcagct cacgagcgat tgcctcgaaa ttttcgccca
     1501 ggtcggcatc ctgtccgacc agatgaacgc ctcgcacacg cgcggcgacg tcagctcgaa
     1561 ggcttatcaa cgcgcccgcg aagagatcca gcaacacctt gcgaagatcc gcttcacggc
     1621 ccgtaccatc gaccgcctgt gcggcgacgt gcaaagccag gtcgcacagg ttcgcgcgat
     1681 cgagcgcaac attctgcaaa tcgtcgtgac gaagagcggc atgccccgcg agcagttcat
     1741 cgaatcgttc aagggtcacg aagccgacct cggctggacg gagcgcgcgg cgcgcggctc
     1801 gtcgtcgttc ggcgcggcgc tcgagcgtca tctgccggcg atccagtcgg aacagcaaaa
     1861 gctgatcgac atcgaggcca ccgtttcgct gccgctcaag catctgaagg aaatcaaccg
     1921 tcagatggtc gcggccgagt cgaagatgcg caaggccaag agcgagatga tcgaggcgaa
     1981 tctgcgtctc gtgatctcga tcgcgaagaa gtatgtgaac cgcggcatgt tgttcctcga
     2041 cctgattcag gaaggcaaca tcggtctgat gaaggcggtg gacaagttcg aataccgccg
     2101 cgggtggaag ttttcgacct acgcaacgtg gtgggttcgc caggctgtga cgcgctcgct
     2161 cgccgatcag gcgcgcacga tccgcgtgcc ggtgcacatg atcgaaacga tcaacaagct
     2221 caaccgcatt tcgcgcgaga tcttgcaaca gaccggtcag gaagcccatc cgtcggtgct
     2281 ggccgaacgc atggaactgt cggaagagaa ggtgcgtggc attctgaaga tcgccaagca
     2341 gccggtctcg atggaaacgc cggtcggcga cgacggcgac gccacgctcg gcgacatgat
     2401 cgaagattcg gcagcttcct cgccggccga cgcagcgatg caggccgact tgcgcgccgc
     2461 catcgacgac gcgctcgact cgctgtcgcc gcgcgaagcg aaggtgctgc ggatgcgtta
     2521 cggcatcaac acgaagtcgg accacacgct cgaagaagtc ggcaagcagt tcgacgtcac
     2581 gcgcgagcgg attcgtcaga tcgaaagcaa ggccatgcgt aagctgatgc atccgagccg
     2641 cgccgatcgt ctgaagtcgt tcctcgaccg ctaagcaagc ggtgcgcttg tcgtgcgcca
     2701 tgcacgacaa gcgcattccc tctccccact caagcaagca acgagatgat cccgccaagg
     2761 ggtgattccc gctgtcagcc aacgcccagc cgcgtagaac gcattacgct cttcctgacg
     2821 cgggttcgaa cgctcgagaa ccagcgtacc gtacgcgaac gacttgaccg ccgagacgcc
     2881 ggttgtcgat gcgccttctg ctgaatcgct tacctcattg ctctgcgcct tatctccctc
     2941 ttgcagcatc ttcggcagag gttgggaagg cctcgtgctg cctcactcga tcgccgtccc
     3001 cacgcaatca ctcgaacaag ccgcccctaa cgcacgcggc tgcgtaactt tcgccggtaa
     3061 cgctccccgc aattgccgat tgcccgcacg gccatcccat ggccgtatgc gttcgacgcg
     3121 cctgccagcg atgaccaatg gcaaggtcca tcccaacacg cgaacgccgc tacgcgcccg
     3181 cgcaacccat ccagaccaca ggcatagtcg ttgcggcgct ccgagtacac tttttacaag
     3241 gagcacatca tgactacgct cgatccccaa gggctcgcat ccgcgggcac gcaaggcgca
     3301 aacatcgtgg gaacaggtat cggcgacggc cccggaccgg acgtcatggc cgcgtcgaca
     3361 ctcgacggaa acaaggtgat gtcgtccgac ggcgaacatg tcggcaagat ctccgacatc
     3421 atgctcgacg tgcgcggcgg acgcgtcgct tatgcggtgc tgtccagcgg cggctttctc
     3481 ggcatgggcg atacgctcca cgccattcca tggagcgcgc tcacgctcga tacggacgaa
     3541 aaatgcttcg tgctcgacgc agccgccgag cacatcaaga atgcacccgg cttcgataag
     3601 gataactggc cttccatggc ggacaccgaa tggggcacct cggtgcacga gtattacaac
     3661 cggccaccct attggagcgc gacgcgagac gtcgtggagg gtcgcgggct ggattccgac
     3721 ctttgatcct ttccactcac cgcaaaacac cataaaacac cgagcttgac ctgaacgatg
     3781 ggcgctgtcg gcaacgacaa cgcccatcgc aacgtaagcg cttctctcct tccgctcagg
     3841 tcttgtcgcc caccgaacgg cttcccactg catcgagctt gctctgcaat gcctgcttgc
     3901 gcacggcgat cttctttcca agtccgacga gatcgcattt cgtgcgccgc aacgccggga
     3961 acaacgcttc ctcttcctct tcgacgtggt gctgaaccag ctcggacatc accttgaagg
     4021 tcgcatcaaa ccctttatcg ccggccttga gcgtctcgaa ctgtgcgatc agcgtcttga
     4081 ccagaaaatg ctcgacataa gcttcgtcga cgtcgagttt gtcgtcgtcc ggcaaggctt
     4141 cctgcgcggc cggatacagc aattcctctt cgatgatcgc gtgcatggtc agctcttcgc
     4201 acgcacgctg cgccagtgcc gcttttgctt ccagatcgtc gtcgccggct ttcttgaatg
     4261 cttcgaagag tttttccact accctctgct cgcaccaggc gcgcaagcgc cggacaatgg
     4321 actttcgaac gacgtcaagg tcgcttgcgg ccaggtccgc gcgagaatgg cgtcgaagtg
     4381 agaccggttt gcgcggacgc cttctctcga tggaaaatcg atgccgattg cggccatcat
     4441 ccaggcgggc ggcaaacgat atctacgcac tacgcactac gcaatacgca ttgcgttttc
     4501 cgcatcacct cgaacgcgca ccgctaaacc acgttcttct ccacgatcgg ccggttctca
     4561 agcacacgcg tgaagctcag cgaaccgaga tcgagcgtcg tgtaacggcc ctgcagaatc
     4621 aattcgctga tgccacggcc cgttgccgga ccttgctgca aaccgtgccc gctgaaacca
     4681 ttggcaaaaa tgcagttatc gacatgcggg tgatagccga tgatcgcatt ctgatcgagc
     4741 acgttgtatt cgtagtagcc ggaccagcag tgttgcacgc gcagcgcttc gaattgcggc
     4801 acgcgatgcg cgagcgtcgg ccagatcacg tcgtcgaata gtgcgtgatc gacttcgtcg
     4861 agcggcaaat cgtcggggtc gttatccgcc gacggcgacg taccgcagat gaatgattgg
     4921 ccttcgggcc ggaaatacac gccgctcgga tcgatcagca acggacaatg gtcgagccgc
     4981 gcggccgacg tgacattgaa aatgctacgg cgtcgtgcga acaccggaag gtcgatgccg
     5041 gccatctgcg cgaccttgcg tgaccaggcg cccgcggcat tcaccacgac gtcgcatgcg
     5101 tagcgctcgc cattcgcgct ctgcacttgc gtgacgcggt taccgtcgcg atgcagcgcg
     5161 gtaacgtcgg cggcgacata gcatgcgccg agcgcctggg ccttcttgcg cagcgcctgc
     5221 acgagaccat agccgtcgaa ccaaccctca ccgctttcgc cataagcacc ggcgacaagg
     5281 tcttccgtat tgagccacgg aaagcgcgct cgcaatgccg ctttgtcgag cagactgata
     5341 tcagcgccaa ggctcttttg aagagcgtga ttctcacgca gcgtcgtttc gccggcagac
     5401 gtggcgagaa aaagatagcc gccctcgtgc agatcgatcg acggtttcgc gccgtcgagt
     5461 tcgagccgct caccgatcga gcgcagaaac tcgatgccga agagcgacat ctgaatggaa
     5521 agcggcgtag agaattgctg gcgaatggat gctgcggaca atgccgacga cgaacgcgca
     5581 taagtcggat cgcgctcgat caccgtgacg ctgactgtcg gatcggacag ccgcaggaag
     5641 tacgcaatcg agctgccgat caccccaccg ccgacgatca cgactttgga actcacgtct
     5701 tgctccacga gtagcgtggc cgctcaggaa cggtgggcgg cgcaaacgga ttgctgaaag
     5761 agattgtgag cagcggcgcg tgaatgcgcc gctgctttcc gctgaaatgc gctagcgctt
     5821 caatgcgcta ctacttaacg aatcagccga tattgaagtc gatgccctgc gcaagcggca
     5881 gcgccgacga atagttgacc gtgttggtcg cgcggcgcat gtaggctttc cacgcatccg
     5941 aacccgattc acggccgccg ccggtttcct tctcgccacc gaacgcgccg ccgatttccg
     6001 cgccgctcgg tccgatgttc acgttcgcga taccgcagtc gctgcccgag tcggacagga
     6061 agcgctcggc ttcgcgcaga tcggttgtga acacgcacga cgacaagccg tgcactgccg
     6121 cattgtttgc ctcgaccgca tccgcgaaat ccgaatagcg cagcacgtag agaatcgggg
     6181 cgaaagtttc cttcagcacc accgctgttt gcgacggcat ttcgacgatg gccggacgca
     6241 cgtagtaacc gccttcatgg ccctttactt cgacgcgctc gccgccgaac actttgccgc
     6301 cttcggctgt agcctgttgc agcgcttcct gcatgcgggc gtacgcttgc ttgtcgatca
     6361 gcgggcccat cagcgtgccc ttttcgagcg gattgccgat cggcaccttg ctgtacaact
     6421 gcttcagacg ttcgatggtt ttgtcataca cgctctcgtg cacgaacaaa cgccgcagcg
     6481 acgtgcaacg ctgccccgcc gtgccgactg ccgagaacag gatgccgcgc atcgcgagtt
     6541 cgtgatccgc ggtttgcgcg acgatacctg cattgttgcc gccgagttcg agcagcgagc
     6601 ggccgaagcg tttcgccact tcaacgccga ccgtgcggcc catttccgtg ctgcccgtcg
     6661 cgctaacgat cgacgcacgc gggtcggcca ccagcttcgc gccgacgtcg cggccaccgt
     6721 tgatcagcgc gctcaggccg gccggcgcgt cgccgaactc cttcagtgcc tccctgagaa
     6781 tctggttgac ggcgagcgcg gtcagcggcg tcttttccga cggtttccag atcaccgcgt
     6841 tgccgcacac cagcgcgagc gccgcattcc acgaccacac cgctgccgga aaattgaacg
     6901 ccgagatgac gacgcacgta cccatgggat gccaggtttc agccatacga tgacccgggc
     6961 gctccgaggc gatcgtcaga ccgtacaact ggcgcgacag gccaaccgcg aagtcgcaga
     7021 tgtcgatcat ttcctgtact tcacccatgc cttcctgcag gatcttgccg gtttcgagcg
     7081 tgatgatgcc gccgagcgcc tgcttcttgt cacgcagacg gttgccgagc agacgcacca
     7141 gttcgccgcg gcgcggcgcc gggacgttgc gccaggccgc gtacgcttct tttgcattag
     7201 tgagggccgc gtcgacttcc gccaccgtgt tgctggccac gcggccgatg agttcaccgg
     7261 taatcggtga atgaactgcg atgtcgccgg cttgcgccgc gtaggcgatg ccgaggtcgg
     7321 cgaggatggt ggaagcgtcc atgagaaccc ctttaatttc cgatgcgaaa caagagtaag
     7381 ttcggaaact atacacgcgg gattttctag cgacaagggt atttgcccgg ggctgggtgg
     7441 cggaccgggg tgaaaaggct tgtgagcttg atgtgctgag gggaatggtt ggcgtcaggg
     7501 cgcgctgcgg gccgttgctg cggaggcgcc gccgggacgc cgtagctcgg tggctagttt
     7561 cttattggaa attatggctt tgactcgcgc cgcgctgctc ggacgtcggc gaccggcctg
     7621 tccaccagca cgcgagcgat acggcagcat ttccagatgt tccgcgtgaa cgttgcccgc
     7681 gacgataccg atatccgtcg cgctatccgc cacgcccttc acgatttcgc cgctcagcac
     7741 ttcgcgcaga tcgatattga cgtcggatga tccataccca cagcaagttg tcgacttcag
     7801 cagaaacctt attcgattac tcatagctcc gttctctcaa gagttggagc ctccactagc
     7861 tttcgcggcg gctcaatgcc tcggcgacgt atttcgacag cacgccggaa atcctttcga
     7921 ggttatcaaa tatgattttc ggagttcttt caaaaacagg ctattcgaac gggtcagcaa
     7981 atgaataatg aaagatagtt gtcgagataa gttcctgtgc agcaacctgg gatgaactca
     8041 aacccgacgc cagccgggca gatggcaggt gaaataaaga ttactgcagt accttgtccc
     8101 gtctcattgg aaccgggatc aaattaaatt caactcgtac ttatgacaag atattacttg
     8161 tcggatattt accgagagat tacgcaaaga caatttttgt ggcccattca caacagcgtg
     8221 gtggcgatgt cgaggggaat gagtggcttg ctcaatatat tttgatattg tctcggcact
     8281 cctggttgac agaaaaaacc cgcgcagagg ccaggaagac atgatgtaat tttttaatca
     8341 ttggagatca aactatgaaa aagtcgcttc tcgcttttgc agcgctgagt acgttcgctg
     8401 gcgttgctaa tgctcagagc aacgtgactc tttacggcat tattgacgaa ggcgtcaacg
     8461 tcgtaactaa ttccggtggg cagcatctat acaatctctc cagcggagaa cttcaagggt
     8521 cacgctgggg cgtgcgcggc accgaagatc tcggcggcgg cttgaaagcg atttttgatc
     8581 tggaaaacgg ttttgacatc aatagcggca agcttggtca aggcggtctg atgttcgggc
     8641 ggcaagcttt cgtcggtctg tcgagtcggc tcggtacggt cactctgggt cggcaatacg
     8701 actcgatcgt cgactacgtg ggtccgctgg aagtcggtga tcaatggggc gggtacattg
     8761 ctgctcaccc gggcgacctg gataacttca acaacgcata tcgcaccaac aacacagtta
     8821 agtacaccag cgcaaactat aacggcctga cgtttggcgg cacatatagc tttggtggtg
     8881 ttgcaggcga tttcacgtcg aaccagatat ggtcgttggg tgccggctat aacaacggtc
     8941 ctctcgtgct cggcgcggcg tatttgaatg cgcgtactcc tgctgcatcg ggtggcctgt
     9001 tcaataacgg cggtgcggtc gcggcagcat cggctgccgt cacgacatcg atttacagcg
     9061 gcttcgcttc ggcgaacacc tatcaggtga tcggcgccgg cggcgcatat acgttcggtg
     9121 cagcaacgat cggtgcgacg tactcgaaca tccggttcga taatctgggc gcgagctcgg
     9181 cttcgccgta caaggggcaa tcggtttcgt tcaacaacgc ggaagtcaac ttcaagtatc
     9241 agttgacccc ggcattcctg gttggcgtag cgtacgacta cacgcgcggc gcgcagattg
     9301 acggtgcttc gcgcgctcag tatcaccagg ctgcggttgg cgtagattac ttcctgtcga
     9361 agcgtaccga tgtgtatgtg acaggtgtgt accagcatgc tcttggcgaa acgctgaatg
     9421 cagcaggcaa agtggtcgcc gcaaccgcaa acatcgatca actgagcccg tcgtcgaacc
     9481 agaaccagtt caccggtcgc atcgggattc gcacgaagtt ctaacgtctc aggtagtaga
     9541 agcaagtcaa cgaaggttca agaggtgctt gcgcacctct tctttacttc atccggtcat
     9601 gagaatcgca tttatcccag agcacggtac gctggacagg ggaagcgagc aatgtttgcc
     9661 ctccagtgcg gacgatcatc gggagttcaa aacggcgtca tccctgtttc gaagtttgaa
     9721 agagtcgcgt tggaaccttg tcgtggcggg ctgggatctg cctgatgtca aaggggacga
     9781 tttactgcgc tggatgcggg ctacttttag cgattctcca cccgtcgtct ttcttacgag
     9841 ccacgatgct gcggccgaca ttgtcaattc tctcaatcaa ggcgcagtcg attacatact
     9901 tgcgcctgtg gagccattgg tgttgcgtgc aaggctgggc gcaatcctga ggcggctttc
     9961 gtccgccccc gatggcctcg tgagagaggg agaagagggc cgttatcagg acattccacc
    10021 gactgtacga ttcggccgct acgtattcca tcagcggcat cattacgtag acattcaggg
    10081 tgaaaaagtg atgcttcgtc caaaggagta tgcgttggcg atgcttctct ttgagaacat
    10141 tggcaagact ctcagtagag agtcgataca tatgaagctc tgggagacaa tggacaattt
    10201 tccgacgagg acgctcgaca cgcatctttc cagactacgg gcaaaactca aactggtcgg
    10261 tggaaatggc ttcgatatct ctcaaatccg gggcgtaggt tatcgattgg aatcctgctg
    10321 agagcgaaat gaagaacggg aggcgggtat gtcgcgcaat tcgtctttgc gctgccgcgc
    10381 gccgtgtttc cggaatcagc cagatttcaa actagtcgcg atcacgatcg cccttcaaca
    10441 tcgtgagccg tgtctgccca tcgagcgtga agtgggtacc aaaacgcacc agccctgcct
    10501 ggcaaagccg gcatttcatc gtaaagctca gcaacatacc cggttcggtg tcttcaatat
    10561 cgatgtcgat cgattccagg cgcggctcgt atttcagcag cgtcgtctcg atcgccccct
    10621 tgagcttgtg cgcggacgac ggcaaatggc gatagatttc cgacaggtcg tcaagtccgt
    10681 aatcgggaag gtggacgagc ccgttacgac ggctgttcag gatgcgctgg atattgtcct
    10741 gcactgagaa gaaggtctgc gttgcgggat cgaagtcatc gaccgccgag ccgtttgcga
    10801 aatgacctgt caccgcctcg aagagaccgg gcccaccgcg ggtcatgctt ctgcctcgat
    10861 catgctgtcg aactccaccg caatgccgct ttcctcggtg aacgacagcg taattccacg
    10921 cgacaattgc tgcgcggcaa cgcgcacgag caattcgcgc gacaggaccg gcaggatctg
    10981 ctgatcgagc agactgtcga cattgcgcgc gccggagtcg ggcaattggc acgcgcgcac
    11041 aagttcgcgt accagcgagt cgtcgccgat gagcggtatg ccaaagcgcc gctccacgcg
    11101 ctgtgccacc ttgtcgagtt tcatccgcac gatcgacgtc aatgccgcgg ccgacagcgg
    11161 ccgatatacg acagtctgga agcgcgcgag caacgccggc tggaagtgct cgaccagcag
    11221 cggtcgaatc gtttccatca gcatcgaagt ggtgatgtcg ccgcctgcct ctgcgacggc
    11281 gtccgtggcg gccatgatct gttcgctgcc gagattcgag gtcatcagaa tcacggtgtt
    11341 gcggaagtcg atcacgcggc cttcaccgtc gcgcatcaag cctcggtcga acacctggta
    11401 gaacaggttc agcacatctc gatgcgcctt ttcgacctca tccagaagaa ccacgctgta
    11461 ggggcgctgg cgcacggctt ccgtcaacac gccccccacc ccgtatccga cataacccgg
    11521 cggagcgccc ttcaactgcg agaccgtgtg cgcttccttg tactcggaca ggttgatggt
    11581 gatcagcgcg cgctcgcccc cgaacatcag gtcggccagc gcgcgagccg tttccgtctt
    11641 gcccacgccg gatggtccca ccagcaaaaa cacgccgagc ggcgcttcct ttgacttgag
    11701 gccggctttg gcggcgcgca gactcttgcc gagtgcagca agcgcgttct cctgacccac
    11761 cacgacgcgg ccgaggcgcg tttcgagcgc gagcagcgtc gcgagttcgt cctccagcaa
    11821 actgccgacc ggcacgccgg tccaatcggc gatcacgcga gccaccgctg cttcgtccac
    11881 ttccgcctga atcagcgcgt cggcgccgtg tccggccagg acttcgtgaa cgacgcggat
    11941 cgattgttgc aggtcgcgac gtacgctttc gtccgcggcc gcatgccatt gttcacgcag
    12001 cctttgcagc gtggcgacgg cgtctttctg ttcggcgaac gtcgattcaa gtgcatcgac
    12061 ttcgaccttg agatgggcga ggcgaagatc gatcgcgagc agccgctcgc ccacctccac
    12121 ggaggtggcg gcatgatccg cgaccaatgc cccatgttcg accaggagcg cagcctgttc
    12181 agcgtgacgc gcggcgatcg cgacgggtgt ggcgtccgaa ctcatacgca cgcgcgccgc
    12241 ggcggtatcg agcaggtcga cagccttgtc gggcaactgg cggcccgtca ggtagcgacg
    12301 cgaaagatcc accgcggccg cgaccgccgc gtcggtgatg tgcacgccgt ggtgcttcgc
    12361 gtaacgcgcc ttcaggccgc gcagcatcag gcatgcgctg tcattgtcgg gttcatccac
    12421 cttgaccatc tggaagcgcc ggtcgagcgc tgcatcgcgc tcgaagtact gtttgtattc
    12481 ggaccaggtg gtcgcggcga tggtccgcag ttcgccgcgc gccagcgccg gcttgagcag
    12541 gttcgcggca tccgcgccgc ctgcggtgtt gcctgcgcca atcagcgtat gggcttcgtc
    12601 gataaatagc aggatcggtg tcggcgaccg ttgtacggct tcgatcacat ttttcagacg
    12661 ctgctcgaat tcgcctttga cgcccgcgcc cccctgcagc aggccgaggt cgagcgttag
    12721 cacgctgacg tcgcggagca ccggcggcac gtcgccgtcg acgatcttca gcgcgagtcc
    12781 ttcgaccagc gcagtcttgc cgacgcccgg ctcgccgacg aggatcggat tgttcttacg
    12841 acggcgcgcg aggatatcga ccatttgccg gatttccaca tcccggccga aaaccggatc
    12901 gattctgccg tcacgcgctt tctgggtgat gtcgatggtg aaacgttgca gcgcgtcaga
    12961 tgccgattgc ccaggcaagg ttgcacgtgc cctggctgcg gaccatgaag gttgatcgcc
    13021 gaatgcgcca tgagacgcgg tttcgtgcat cgcggagccg gccatgccat tgccgggcac
    13081 gccacccatg ctgtgacgcg ccgtatcgag gtcgccgtcc ggctgtacgg agacgctcgc
    13141 cgcttctacc gaaatcctgt cgagcgcggg catcaagcgt ccgatctgcg cagccgacac
    13201 ggagagcagc ggccaggcat ccggcgcgcg tagcagatgc ggcgcgtcgg cgagcgccgc
    13261 cagcagatgc gccgaccgga tcgacgtggc gcccgcttca agcgaggcac acatccacgc
    13321 tgcttccagc cattggccga gccgatggga caggcctggt ttgccgcgca acgcgtgcgg
    13381 aagccggtcg atcgcgctga gcaggccgtt ccagatgccg tccgcgtcga gttcatagtg
    13441 gcgcacgatc gccacgagat cgccttctcc caactccagt agtttgatca accagtgttc
    13501 gatttcaaca tcgcgatggg cgcgcgtctc gcacagactc gctgcttcgg cgagcgcgcg
    13561 ggcgcaatga tcgttgaggc ggcgcagcaa gggggaaagg tctcgagtgt tcatggcaac
    13621 ggaaaagcag gcgcgataca gatagaaagg cgcacgaacc aaaatgacga cggcccgtgc
    13681 gttaaaaacg acgccctccg ggagacgtga agaggcgccg tgatgggtcc ttctatgaac
    13741 gttcgttcca gctatcggcg tggatgatgt tgccgtcctt gtacgcccag gtgattttct
    13801 cgtaacgcag ttcgacgtct tccagatggt tgtgcttctc ataggccggg ttcttgatgt
    13861 ccagcatctt cggcttgacc gctacgacct tcacgttgtc gagcttcgtg ttgaagtatt
    13921 ccttttcctt gcccgcgtcg tcgatcttgt accacttgat ctcgatggac ttgagcgtct
    13981 gaccgctcgt caccgccttg tacaaatatc aactgctgct ggaatatttc actttcccgg
    14041 aaaaattcat gttcgtggat ctcatcggtc tcgacatgcc cgccatcccg ctggcggcgg
    14101 aatacttcga cgtcgaagtg gttttggcgc aaccgtatcc ggacgacgca cgcttctccg
    14161 ccgacaacgt gcgcctctac tgcacgccca tcgtcaatct gttcgcgctg gaagcggacc
    14221 cgatcacggc gtcgcagcat gagacggaat accgcgtaca cgcgcgtgaa caacggggcg
    14281 atcacgtcga catctattcg gtcgagagcg tgcggggttt cgaagcgggc acgggccgac
    14341 gcttcgaata tgcgccgttc accgcattcc gtcatcgcgg cggcatgttg cgccatgaaa
    14401 tgccggaacg gtatttccac acgcgtacgc gacgtggccc atccggacgc ttcgacacct
    14461 ggatcgtcct gggtggtcat gcgtgggaac agcaggccac gctaacgaag gaaacgctgt
    14521 cgctgtccgt caccggcacg aacggcatgg tgccgcgcaa aggcttgcgc gacgcgggga
    14581 tcaaccgcat gcgcggcggc ttcaccaaca tcggtgcggt gcgcaacctg accgcgccga
    14641 ccctgcctgt gtatccaccc actggggacc gcttccagtg gcgcgtgctg tcgcatctcg
    14701 cgccgaacta cctgtcgctg ctcaatgcgg aaatcttgcg agggagcctc gcgctgtacg
    14761 actggactca gggtgaactc aacaagcgca ggattgacgc gatcaccgac gtacaacacc
    14821 ggctgttgca aaagctcgtc aagggcggcc tgcaacgtgg cgtcgagatc gaagtaacgc
    14881 tcgacagcgc cgcattcgcc ggcgacggcg acatcgaact gttcggcggg atgctgaacc
    14941 gctttcttgc gctgtacgcg acgctgaacc tgttcacgaa gctggtgatc gtgtcgcaac
    15001 ccactggacg ccgcatggaa tggcccaata cgaaaggcga aggagcgccg ttttgaacgg
    15061 accgcaatgg agggcagcgg agcaggcgct cgcgctggag tttgcacact acgccgaccg
    15121 gccgttgctg ccggcgttgc tcgtcgacgc gacgcggatg aattttttcc gcttctgcga
    15181 gctgatcgaa ctggccgcac ccgatcggcc gcccatcggc acgaccgatt cgccggcgtc
    15241 ggagccggtg cgctttcgct cgcgggcgcg cctcggcttt ccgaatcgcg agatcgatgc
    15301 ggtcgaatac gacctcgacc atccggcgat gccgtccgtc gtacgcacga cttttctcgg
    15361 actgtacggt gtcgatgcac gcatgccgtc gtactttgtc gacgaagtcg cgcagaatcg
    15421 cgagggtgcc gaatcgatgt cggcattcct cgatatcttc catcaccggg tggtcacgca
    15481 gtattaccgc gtgtggcgca agtaccgata tcccgccggt ttcaggaacg acggaaccga
    15541 tgaaatctcg cgttacctgc tgagtttcgc cggcctcggc atcgcttcac ccgaagccgc
    15601 cgcggccggc tcacaaggac ttccctcggg cctgggcacg cgcaagctgc tgtcgatgct
    15661 gggactcgca agccagaaga cgcgcacggc tgaaggtctg gttggcgtgt tgcagcacgc
    15721 ggtgccggac gcgcgaatca cggtggagga gttctacccg gtgtggatca gtgcggccga
    15781 ttatccgcgg actccgctag gtgaggcatg tctgcttggc cgtggatttt tcgaccgcgc
    15841 aaacgcggtg cggatcgtca tcaccccgca aacacgcgaa tcggtactcg ggctgatgcc
    15901 cgggcgggca aatcacagtg aggtgatggc gctgctgcgc ttttacctgg ggtacgaagc
    15961 gcgggcacga ctcgaaatgc atgtggctcc cgagttgatg ccggcgcaag cgctgaattc
    16021 gaatcaggtc agcctcggtt atacgaccca actcccgcgg cctcgtatgg aggacccgcc
    16081 tgccccggtt acgcgagtgc aactgggcac gtggacggga agcagcgacc gccacgccgc
    16141 tcgacaacat tcgatgcact gatgcactga tgcactgatg cactgatgca gcgaattatg
    16201 aacatggaca cgaaaaaagg atcgacagaa tgatgcggat acttcgaatc ggcgccactg
    16261 cgttgatcat tcttgccatt gcaggttgcg gcgtagggca ggcagtgaaa gacggcacgg
    16321 tggacgctgc gaaatgggcg ttcacgacgc aggtcaaaac aatgaacatg gacctcgtca
    16381 gccggtcgtc actaaacgca aacggcgcgg ggcaatcatt gtcgacggtg gtgcggatct
    16441 atcaattgaa aacgccacag gcattccagc aactcgacta cacccagttg cagacaaat
//
LOCUS       DATASH010000253         6152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5573004,
            whole genome shotgun sequence.
ACCESSION   DATASH010000253 DATASH010000000
VERSION     DATASH010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6152
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5573004"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="VHV99_07090"
     CDS             <1..895
                     /locus_tag="VHV99_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX3635239.1"
                     /translation="QVAPDISGLISSVEVVDNQQVKQGQVLFVIDQARYALALRQAQA
                     TAQQRRATLDQARREDARNRQLGNLVAAEVSEESRSRVETAEAALADANVAIDTARLN
                     LQRTTIVSPVDGYLNDRAPRTGEFVSAGRAVLAVVDMHSFRVDGYFEETKMHGIDIGQ
                     RVDIQVMGEPTVLRGHVQSIVAGIEDRDRTQGSNLLPNVNPAFSWVRLAQRIPVRVAL
                     DEVPADFRLIAGRTATVSMRELSPTGKTRPAAGASGAGDSSVDSPTAAASASATSAVP
                     TVSAASATQPASAAVMSGASQ"
     gene            892..2436
                     /locus_tag="VHV99_07095"
     CDS             892..2436
                     /locus_tag="VHV99_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEX3635240.1"
                     /translation="MKLSRALPLLPRLPLLPLLPLAVALNGCVNVGPDYALPKQALVN
                     APLANAPIEGADAKLTTRQDVPAVWWKLYDDPVLNSLVDEALQSNTDLRVAAANLARS
                     REALGVAQAQGGFSGKTSAAFERAQESAEQYLLFNKLPVANEGDIGISISYEIDLFGK
                     LRRGVEAAAADTESVQAAGDLARITVVADVVRSYVEQCSAAEDLRIAQQSLALQKQRV
                     DVSRRLRDAGRGNQPDVTRGQTQVDTLSADIPRYTARRQIAQYRLAALLARAPSDLPP
                     AVLACNTLPQIRQPIPVGDGAALLKRRPDVREAERQLAASTARIGVATGALYPTVSIG
                     ASAGLTGILEDLGTSPTARWAFGPLISWTFPSNGARGRVREAAASSNVALAKFDGVVL
                     TALRETETNLATYASDSARADALRAALKSATESADETHRLYQAGRESFISDLDATRTL
                     TSTKSQVAAAEGQVAIDQVNLFLALGGGWEIDAQTAPEDAGPGAATRAVAAAAKVVPS
                     AATKAP"
     gene            complement(2900..3361)
                     /locus_tag="VHV99_07100"
     CDS             complement(2900..3361)
                     /locus_tag="VHV99_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX3635241.1"
                     /translation="MGSLNINGRAVAVQAEPDMPLLWVLRCDLGMTGTKFGCGVGICG
                     ACTIHLDGKAGLACQTPMSSLRSQKITTIEGVQGAEAQALKAAWIDLDVVQCGYCQSA
                     QLMAACALLKQKPDPTDADIDEAMSNIVCRCGTYPRVRAAIHQAAAKRRPA"
     gene            complement(3388..5658)
                     /locus_tag="VHV99_07105"
     CDS             complement(3388..5658)
                     /locus_tag="VHV99_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX3635242.1"
                     /translation="MLKRRTFLLGGAGALGALLVGWSVLPPGQRLTTATPLPAEGSQL
                     ALNGWVKIAADNSVTIMMCKAEMGQGIHTGLAMLLAEELDADWSHVRVENAPIDKIYN
                     SVQNIIDDLPFRPDDDSTVKRATVWLTRKLVRDAGTMMTGGSSSINDLWTPMREAGAS
                     ARAMLIGAAAGQWKVPPGECRTEGGYVLHASGHRASFGELSTLAAQQPLPRKVALKDP
                     ADFKLIGKPMRRIEAASKINGTARFGIDALPDRLIYASVVMCPTLGGTVAHFDATPAT
                     RMPGFIKAFALPPYAGGSGGVAVIADNPFRAMNAVSAINVAWNNGPAATLSSADVDAH
                     LAQVLDSSDGHAYYHRGDADAALSGAARTLTAEYHAPYLAHGAVEPVNCTVQVADGAA
                     TVWVSTQMPALARLHVAKVLDLDTDKVDVQTQLLGGAFGRRLELDFIAQAAAIAREGG
                     GRPVQTIWSRAEDFTHDFYRPACVSRLKAGFDKDGKLVAWHATSASQAIVPDALARYY
                     GAPRIPLDKTTCEGAFDQPYEWPTARVAHKIVELPVPIGFWRSVGHSHQAFFTEGFTD
                     ELAVATGQDPIAFRASLLAQHPRHLAVLRRVAALSDWGRPMAHRADGSKQARGVALHE
                     AFGSVVAQVVEVSVGPNKQIRVHRVVCVIDCGLPVNPNLIRQQMESGIVFGLSTALQD
                     EITINAGQVTQQNFVDFPVVRMNDCPVIETDIMPSQMHPQGVGELGVPPVAPAVANAI
                     FALTGQRLRALPLRLA"
BASE COUNT         1058 a         1980 c         1995 g         1119 t
ORIGIN      
        1 ccaggttgcg ccggatatct cggggctgat ctcatcggtc gaagtggtgg acaaccagca
       61 ggtcaaacaa ggccaggtgc tgttcgtgat cgatcaggcc cgctacgcgc tcgcactgcg
      121 ccaggcgcag gccaccgcgc agcagcgccg cgccaccctc gaccaggcgc gccgcgaaga
      181 tgcgcgcaat cgccagctcg gcaacctcgt cgcggcggaa gtctcggaag aaagccgctc
      241 gcgtgtcgaa acggcggagg ccgcactcgc cgatgcgaac gtcgcgatcg ataccgccag
      301 gctcaatctg caacggacca cgatcgtgag tccagtggac ggttatctga acgaccgcgc
      361 gccgcgtacc ggcgagttcg tctccgcggg gcgcgcggtg ctggcggtgg tggatatgca
      421 ttcgttccgc gtcgacgggt atttcgaaga aaccaagatg cacggcatcg acatcggcca
      481 gcgagtcgat attcaggtaa tgggcgagcc gactgtactg cgcggtcatg tgcaaagcat
      541 tgtcgcgggg attgaagacc gtgaccgcac gcaagggtcg aatctgctgc cgaatgtgaa
      601 tcccgccttt agctgggtgc ggctcgcgca gcggattccg gtgcgcgtgg cgctcgacga
      661 agtgcccgcc gactttcgtc tgatcgccgg acgcacggcg acggtgtcaa tgcgcgagct
      721 gtcgccgacc ggtaaaacac gtccggcggc gggggcttcg ggggcggggg attcgtcggt
      781 ggattcgcct acggctgcgg cttcggcttc ggctacgtct gctgttccga cggtctcagc
      841 tgcttcggct acacagccgg catctgctgc cgtcatgtcg ggcgcatcgc aatgaagctg
      901 tcgcgtgccc tgcccttatt gccgcggctg ccgttgttgc cgttgttgcc attagccgtg
      961 gcgctcaacg gctgcgtgaa cgtcggccct gattacgccc tgccgaagca ggcgctggtc
     1021 aacgcgcccc tcgccaatgc gccgatcgaa ggcgccgacg ccaaactgac cactcggcag
     1081 gacgtgcccg cggtctggtg gaagctctat gacgatccgg tactgaacag cctcgtcgac
     1141 gaagcgctgc aatcgaatac cgacttacgc gtcgcggcgg ccaacctcgc gcgttcgcgt
     1201 gaagcgctcg gcgtcgcgca ggcgcaaggc ggcttctccg gcaagacgtc ggcggccttc
     1261 gagcgggctc aggaatcggc cgagcagtat ctgctcttca acaaactgcc ggtcgctaac
     1321 gaaggcgaca tcggtatcag catctcgtac gaaatcgatc tgttcggcaa gctgcggcgg
     1381 ggtgtcgaag cggcggcggc cgatacggaa tccgtgcagg cggccggcga tctcgcgcgg
     1441 atcacggtgg tggccgacgt ggtgcgctcg tatgtcgaac aatgctcagc cgccgaggac
     1501 ctccgcatcg cgcagcagtc gctggcgttg caaaagcaac gcgtggacgt gtcgcgccgg
     1561 ttgcgcgacg cgggccgcgg caatcagcct gacgtgacac gcgggcagac ccaagtcgat
     1621 accttgtcgg cggatattcc gcgctacacg gcgcgccgcc agatcgcgca ataccggctc
     1681 gcggcactgc tggcgcgcgc accgtccgat ttgccgccgg ccgtgctggc ctgcaacacg
     1741 ctgccgcaaa tccgtcagcc gatcccggtc ggtgacggcg ccgcgctgct caagcgccgc
     1801 ccggacgtgc gcgaggccga acgccaactg gcggcttcga cggcgcgcat cggcgttgca
     1861 accggcgcgt tatatccaac ggtgagcatc ggcgcgtcgg cgggcttgac cgggattctc
     1921 gaggacctcg gcacgtcgcc gacggcccgg tgggccttcg gtccgttaat cagttggacg
     1981 tttccgagca atggcgcgcg tggccgtgtc cgcgaagcgg cggcgtcgag caacgtggcg
     2041 ctggcgaagt tcgacggcgt ggtgctgacc gcgttgcgcg aaaccgagac caacctcgcg
     2101 acttatgcgt cggattccgc ccgcgccgac gctctgcgtg ccgcgttgaa atcggccacg
     2161 gagtcggcgg atgagacgca tcggctgtat caagcgggac gcgagtcgtt catttcggat
     2221 ctcgatgcga cgcgcacgct gacttcgacg aagtctcagg tcgcggccgc ggaaggtcag
     2281 gtggcgatcg atcaggtcaa cctgttcctc gcgcttggcg gtggctggga gatcgatgcg
     2341 cagacggcgc cggaggatgc ggggcctggc gctgcgactc gcgctgttgc cgcggcggct
     2401 aaggtcgtgc catcggcggc cactaaagcg ccctgacagg tttttgatcg actcgggtgg
     2461 ggtctcgggt gggccgctcc aatgccaatc cagtgcccgg ggatgccgct gttctggaag
     2521 gcgccgtttt cgggcaggtg ggcggcaaac cggcgatggc ctgaacccta ccgcctcgcc
     2581 tcatccgggt aaatcactat caccggaaag ctttctttta cctttcaact caggttaaat
     2641 acgagcctca acaccacgtt atggggtcac gtatgtcgca ttttctgcaa ggcgcagcca
     2701 ccttgggcgg ttgtatcggc gttttcacgg tagtcgtcgc ggtcgtggag atgctggcga
     2761 attaagttcg cctaatgcct gattggcgcg tgattgacgt gattaccgtg attgacgtga
     2821 ttaccgcgtg attaccgcgt gattggcaag tgaccgcctt acgtgggcgc ctgcgcccgc
     2881 cgcagcattt tttcagcccc taagcaggcc gccgcttcgc ggccgcctgg tgaatcgccg
     2941 cccgcaccct cggatacgtg ccgcaacggc acacgatgtt ggacatcgcc tcgtcgatgt
     3001 ccgcatcggt gggatcaggc ttctgcttga gcagcgcgca ggcggccatc aactgcgcgc
     3061 tctggcaata accgcactgc accacgtcga gatcgatcca cgccgctttt agcgcctgcg
     3121 cttcggcgcc ctgcacgcct tcgatggtcg tgatcttctg cgagcgcaag ctcgacatgg
     3181 gcgtctggca ggcaagaccc gccttgccgt ccagatggat cgtgcatgcg ccgcaaatgc
     3241 ccacgccgca gccgaacttg gtgccggtca tgccgaggtc gcagcgcagc acccacagaa
     3301 gcggcatatc cggttcggct tgcacggcca ccgcccggcc gttgatgttc agcgatccca
     3361 tgcttgtctc cgtcaatccg atgcgtttca tgcaagcctc aacggcaatg cgcgcaagcg
     3421 ctgaccggtc agcgcgaata tcgcgttggc cactgccggc gcgaccggcg gcacgcccag
     3481 ttcgccgacg ccttgcgggt gcatctggct cggcatgatg tcggtttcga tcaccgggca
     3541 atcgttcatc cgcacgacgg ggaagtcgac gaagttctgc tgcgtcacct gtccggcgtt
     3601 gatggtgatt tcgtcctgca acgctgtcga gaggccaaac acgataccgc tctccatctg
     3661 ctgacgaatc aggttcggat tcaccggcag tccacagtcg atcacgcaga ccacccgatg
     3721 cacgcgaatc tgcttattcg gaccgaccga tacctccacc acttgcgcga cgacactgcc
     3781 gaacgcttcg tgcagcgcaa cgccacgcgc ctgcttgctg ccgtcggcgc ggtgagccat
     3841 tggacggccc caatccgata gcgcggcaac gcgccttaac acggccaggt gacgcggatg
     3901 ctgcgcgagc agcgacgcgc gaaacgcaat cgggtcctga ccggtggcta cggcgagttc
     3961 gtcggtaaaa ccctcggtga aaaacgcctg atgagaatgg ccgaccgatc gccagaatcc
     4021 aatcggcacc ggcaattcga cgatcttgtg tgccacgcgc gcggtgggcc attcgtaggg
     4081 ctgatcgaac gcgccttcac aggtggtctt gtcgagcgga atgcggggcg cgccgtagta
     4141 acgcgcaagc gcatccggca cgatcgcctg actcgcggac gtggcatgcc acgcgaccag
     4201 cttgccgtcc ttgtcgaaac cggccttcag gcgcgatacg cacgccggac gataaaaatc
     4261 atgggtgaag tcctccgcac gcgaccagat tgtctgcacc gggcgaccac cgccctcgcg
     4321 tgcaatcgcc gcggcttgcg cgatgaaatc gagttcgaga cggcgcccga aagcaccgcc
     4381 gagcagttgc gtttgcacgt cgaccttgtc ggtgtcgagg tccagcacct tcgccacgtg
     4441 cagccgcgcg agggccggca tttgcgtcga gacccagacg gtggccgcgc cgtccgccac
     4501 ctggaccgtg caattcaccg gttcgaccgc gccgtgcgcg agatacggtg cgtgatactc
     4561 ggcggtgagg gttcgcgccg cgccgctcaa tgcggcgtcg gcgtctccgc gatggtagta
     4621 ggcatggccg tcgctgctgt ccagtacctg cgcgaggtgg gcgtcgacat ccgcgctcga
     4681 tagcgtggcc gcgggaccgt tgttccacgc tacgttgatg gcgctgaccg cgttcatcgc
     4741 gcggaacggg ttgtccgcga tgaccgcgac gccgccgctg ccaccggcgt agggcggtaa
     4801 cgcgaacgcc ttgataaagc ccggcattct ggtggcaggc gtggcgtcga aatgcgccac
     4861 cgtgcccccc agtgtcggac acatcaccac gctcgcatag atgagacggt cgggcagcgc
     4921 gtcgatgcca aaccgggcag tcccgttgat tttcgacgcg gcttcgatgc gtcgcatcgg
     4981 cttgccgatc agtttgaagt ccgcaggatc tttcagcgcg accttgcgcg gcaggggttg
     5041 ttgcgcggcc aacgtggaga gttcgccgaa gctcgccctg tggcctgatg cgtgcagtac
     5101 gtagccgcct tccgtacggc attcgccggg cggcaccttc cattgcccgg ccgccgcgcc
     5161 gatcagcatc gcgcgcgccg aggcaccggc ttcgcgcatc ggcgtccata gatcgttgat
     5221 gctcgatgaa ccgcccgtca tcatcgtgcc ggcgtcgcgc actagcttgc gggtcagcca
     5281 aaccgtggcg cgcttgaccg tgctgtcgtc gtccggacga aacggcagat cgtcgatgat
     5341 gttctgcacg ctgttgtaga tcttgtcgat cggcgcgttt tccactcgaa cgtgcgacca
     5401 gtcggcatcc agttcctcgg ccagcagcat cgcgaggccg gtgtggatgc cctggcccat
     5461 ttccgctttg cacatcatga tcgtgacgct gttgtccgcg gcgattttga cccagccgtt
     5521 gagcgcgagc tggctgcctt cggccggcag cggcgtggcg gtggtcaggc gctggcccgg
     5581 cggcagcacc gaccagccga ccagcaacgc gccgagcgcg ccggcgccgc ctagcaggaa
     5641 ggttctgcgc tttaacatcg tatggctcga tcagcttgca cggtggcggg gaagctgtgg
     5701 aagcgcggcg cacgctcggg atttagtact tcagaacact aataagacga gcgagcgggg
     5761 gtgcaactga aattcggacg tttaagtcat tcagtgccat aggggcagat gagcgctgaa
     5821 gaaggtggaa gcgcccgcgc atgccgtgtg atgttccatt aaatgtcatc cggcgaacag
     5881 cgacacgctg caatgcaacc tcgccaaatc cgcggttgga caagatgcga cgtattcccc
     5941 tgcgggcgcc attgttgatc gaccgcaaac ggagccgcta tacaaccctc atggcccacg
     6001 cgctcgggat ataaacaacg gcttcccgga gctgtcacgg catgcccggg cgctgaacgc
     6061 gccctgaaat catccgttct cccgaatcat ctcaatcaac gcccgcagcc ccgccggcac
     6121 atggcgccgc cccggatagt agagacacag cc
//
LOCUS       DATASH010000254         7620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4780198,
            whole genome shotgun sequence.
ACCESSION   DATASH010000254 DATASH010000000
VERSION     DATASH010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7620
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4780198"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(607..1203)
                     /gene="ampD"
                     /locus_tag="VHV99_07110"
     CDS             complement(607..1203)
                     /gene="ampD"
                     /locus_tag="VHV99_07110"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HEX3635243.1"
                     /translation="MSVAFTVDADGWVSAARKLPSPNFEARPAGAVPTLIVVHNISLP
                     PNEFGGTAIAELFLNTLDCDAHPYYDTHLRGVRVSAHFVIHRDGALEQFVSCNERAWH
                     AGPSNFFGRERCNDFSIGIELEGSDTTAFEAAQYRTLGALVTALKVRYPVEGLAGHSD
                     IAPGRKTDPGPHFEWQRLQRDTALADQYFPYLKFSRTP"
     gene            complement(1200..1508)
                     /locus_tag="VHV99_07115"
     CDS             complement(1200..1508)
                     /locus_tag="VHV99_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP0621 family protein"
                     /protein_id="HEX3635244.1"
                     /translation="MRQIFLLILLFIVGQWVVKALRRHDAQASQRTGAGAGANANGAA
                     RGPHAPQAQAQLAEPMIRCAECGVHAPKSDSVVVAGQPFCSAAHAQRHGAHPTGRDAR
                     "
     gene            complement(1519..2445)
                     /gene="ccsA"
                     /locus_tag="VHV99_07120"
     CDS             complement(1519..2445)
                     /gene="ccsA"
                     /locus_tag="VHV99_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEX3635245.1"
                     /translation="MDIVLYALTALLYGGLAVAGWRSHRHAALRPMLESVPPLPVAAG
                     VSAPSAASGMGASGRALLFVALLAHGVLLHTTIFPQNAMVFGFAFALSAMFWLGAGIY
                     WIESFFFPLDGLRLLVLPLACVASLLPLGFGGVRVLPYSAAPMFKLHFLIANIAYGLF
                     AIAALHAILMLLVERRLHAMRGGVSQRNAAAAGNGWLSSWLDTLPPLLTLEKLLFRLI
                     GAGFVLLTLTLLSGIIFSEQLIDRALQLDHKTVFAILSWVMFGALLTARKVSGWRGRA
                     ALRWVLASFVALLLAYVGSRFVFEVLLHRAVV"
     gene            2527..3894
                     /gene="ffh"
                     /locus_tag="VHV99_07125"
     CDS             2527..3894
                     /gene="ffh"
                     /locus_tag="VHV99_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX3635246.1"
                     /translation="MLDNLTQRMARVVKTLRGEARLTEANTQEMLREVRLALLEADVA
                     LPVVREFIAKVKEKALGEEVISSLSPGQALVGVVQRELTAIIGGDYEGKAVELNLAVT
                     PPAVILMAGLQGAGKTTTVGKLAKLLREKYKKKVLTVSCDVYRPAAIAQLKTVTEQVG
                     ADFFPSEPDQKPVDIARAAVDWAKRHYHDVLLVDTAGRLGIDEAMMQEIAALHSELKP
                     AETLFVVDAMLGQDAVNTAKAFSDALPLTGVVLTKLDGDSRGGAALSVRHVTGKPIKF
                     VGVAEKLDGLEIFYPDRMANRILGMGDILALVEEAQRGVDVQAAQKLADKVKKGGDFD
                     LNDFRAQLTQMKSMGGLSSLMDKLPAQFQQAAAGANMGMAESQMRRMEGIINSMTPLE
                     RAKPELIKATRKRRIAAGAGVQVQEVNRMLNQYDQMRTMMKKLKGGNLQKMMRGMKGM
                     MPGMR"
     gene            4044..4595
                     /locus_tag="VHV99_07130"
     CDS             4044..4595
                     /locus_tag="VHV99_07130"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016346290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HEX3635247.1"
                     /translation="MNREEALHIFSHSEEIVSADDVNASISGMAAAIRNEMSEDFPLV
                     LSVMGGAAVFTGMLLPHLDFPLEFDYIHLTRYRNTTKGGNEMQWRVAPAESVKDRVVL
                     VLDDILDEGETMAAIRDRILAMGAKRFLSAVLCEKIIPKAKPLRPDYCGFEVPDRYVF
                     GCGMDAKGYWRNLPTIRALTEGA"
     gene            complement(4799..5398)
                     /locus_tag="VHV99_07135"
     CDS             complement(4799..5398)
                     /locus_tag="VHV99_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012363042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEX3635248.1"
                     /translation="MEYTFVSATVLLILITDPLGNIPLFISCLRGVSPKRRTIVILRE
                     VAIAFAILLVFMVVGDGFLRMMSLTDQSLRIGGGIVLFLIALRMVFPHPDGPLGGDTR
                     GGEPLIVPLAIPALAGPSALATVMLLTSQAPGKMLEWIGALTVTMIVCAIVLMLAERI
                     QAWLGERAMMAFERLMGLVLVAISVEMMLGGIRSFVHQL"
     gene            complement(5411..7147)
                     /locus_tag="VHV99_07140"
     CDS             complement(5411..7147)
                     /locus_tag="VHV99_07140"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX3635249.1"
                     /translation="MKASRFFIGTLKEAPADAEIVSHKLMVRAGMIRRIAGGIYNYLP
                     IGLRSIRKVEAIVREEMNRAGGIELLMPSVQPAELWQESGRWEKYGPELLRFKDRKQS
                     DFVFGPTHEEVITDIARGQIKSYRQLPVNFYQIQTKFRDEIRPRFGVMRGREFIMKDA
                     YSFDKDIEGLRESYRKMYDAYVRIFTRLGLDFRAVAADNGSIGGSGSHEFHVIAETGE
                     DAIAYCPTSDFAANVEAAEALPLYAQRAAPTEEMKKTATPGKAKCEAVAELLNIPLER
                     TIKSIVLATENEGAAPTIWLLMLRGDHDLNEIKAAKLPGLADFRMATEAEIIETFGTP
                     PGYLGPINTKKPVKVVADSTVANMSDFVVGSNEVDYHTTGVNWGRDLPEPIVADIRNV
                     KKGDPSPDGKGVLDICRGIEVGHVFQLGTKYSEAMNATCLDETGKPRQMEMGCYGIGV
                     TRILGAAIEQNFDEKGIIWPDSIAPFEVVLCPMGYDRSEAVREQADKLYAALLEAGVD
                     VILDDRGERPGVMFADWELIGVPHRLVIGDRGLKDGKLEYQGRRDVEATLLPVDDAAQ
                     TLIGKVRAALAQ"
     gene            7531..>7620
                     /locus_tag="VHV99_07145"
     CDS             7531..>7620
                     /locus_tag="VHV99_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011852215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEX3635250.1"
                     /translation="MLDREGFRPNVGIILLNAHNEVFWGKRLRE"
BASE COUNT         1524 a         2385 c         2371 g         1340 t
ORIGIN      
        1 gcgccttgtg cctggccgaa ggcctggcca gtcggtgagc cctcataccg ggtcgtcacg
       61 ttgtcggtgg tttgcatgtg caaagctcct ggtcctggaa atgttgcggg aagatccgcg
      121 gattaaaacg aacgctgcgc cgccgcgtgc gcaagcgatg gggtgcagga aaaaggccgg
      181 ctgggccggg tggcgggatg cgacagtgat gaagcctgat tcgaattgat aacggtcttc
      241 atcatgtgtg tcgcgatcac ttgctatgcc cttttcctga atgcttctgg atgccgccgg
      301 aggtctctac cggccgcgcc gcaggaattt ttttcgctgc ctggaatgct tgcggcgcca
      361 tgctcgggga gcggggcgcc gctgacttat gcactcagtt atgcacagag ttatacacac
      421 cagacacaag atatagtgcg aaactgaatt tctggcacca agtatagtgt agtttcaaga
      481 caggtcaaat cgatttattt gctttcgaag tcttgacttt tgcatcgcgc gacgcgaagc
      541 cgactccccg gagaacaagg cggcgccttg ctctcctcgc atcacgcgaa ctcgcgcgaa
      601 gggtcgttac ggcgttctgg aaaacttgag ataggggaag tactgatccg ccaacgcggt
      661 gtcgcgctgc agacgctgcc actcgaaatg aggccctgga tcggtcttgc ggccgggggc
      721 aatgtccgag tgaccggcga gcccttcgac cggatagcgg accttcagcg ccgtcacaag
      781 tgcaccgagt gtgcggtatt gcgccgcttc gaaagcagtc gtgtcgctgc cttccagttc
      841 gatgccgatc gagaaatcat tgcagcgctc gcggccgaaa aaattcgacg gcccggcgtg
      901 ccatgcgcgc tcgttgcagg aaacgaattg ctcgagcgcg ccgtcgcgat ggatcacgaa
      961 atgcgccgac acccgcacgc cgcgcagatg cgtgtcgtag tacgggtggg cgtcgcaatc
     1021 gagtgtgttc aggaacagtt cggcgatcgc cgtgccgccg aactcgttgg gcggcaggct
     1081 gatgttgtgc acgacgatca gcgtcggcac ggccccggcg ggccgcgctt cgaaattcgg
     1141 cgatggcagt ttgcgtgcgg cgctgaccca accgtcggca tcgacggtga acgcgacgct
     1201 catcgggcgt cgcggcccgt gggatgcgca ccgtgacgct gcgcgtgcgc ggcgctgcag
     1261 aacggctgcc cggctaccac cacggagtcg ctcttcggtg cgtgcacgcc gcactcggca
     1321 caacggatca tcggctcggc gagttgcgct tgcgcttgcg gcgcatgggg tccccgcgcg
     1381 gcgccgttgg cgttcgcgcc agcgccagcg ccggtgcgtt gcgacgcttg cgcgtcgtga
     1441 cgacgcagcg ccttgaccac ccattggcca acgatgaaca gcaggatcag cagaaaaatt
     1501 tgtcgcatgg caggacactc acactacagc acggtgcaac agcacctcga atacaaaacg
     1561 gctgccgacg tacgccagca gcagcgcgac gaacgacgcc agcacccagc gcaatgccgc
     1621 acggccgcgc cagccggaaa ccttgcgcgc ggtcagcagc gcgccgaaca tcacccagga
     1681 aagaatcgca aagacggtct tgtgatcgag ctgcaatgcg cggtcgatca gttgctcgct
     1741 aaagatgatg cccgagagca gcgtcagcgt gagcagcacg aagccggcgc cgatcagacg
     1801 gaacagcagc ttttccagtg tcagcagcgg cggcaaggta tcgagccagc tcgacagcca
     1861 gccgttgccc gccgcggccg cattgcgctg cgacactccg ccgcgcatcg catgcagacg
     1921 ccgctcgacc agcagcatca gaatggcgtg cagcgccgcg atcgcgaaca aaccgtatgc
     1981 gatgttggcg atcaggaagt gcagcttgaa cattggcgca gcggaatacg gcagcacgcg
     2041 cacgccgccg aaccccagcg gcaataatga agcgacgcag gcgagcggca gcaccagcag
     2101 gcgcaagccg tcgagcggaa agaaaaaact ctcgatccag tagatgccgg cgccgagcca
     2161 gaacatcgcc gacagcgcga acgcgaagcc gaacaccatc gcattctgcg ggaagatggt
     2221 ggtatgcagc agcacaccgt gcgcgagcag cgcgacaaac agcaacgcgc ggcccgacgc
     2281 gcccatgccc gaggcggccg atggcgccga aacgccagcg gcaacaggca gcggcggcac
     2341 gctttcgagc atggggcgca gggcggcgtg ccggtgcgag cgccagccgg ccacggcgag
     2401 accgccatag aggagcgcag tgagggcata cagtacaata tccatattcc gagtttacac
     2461 taggcccctg ctccgcgacg tctcccgctt cgcgcgctgc tccggccgca cagttcatcg
     2521 ctccccatgc tcgacaatct gactcaacgg atggcgcgcg tcgtcaagac gctgcgcggc
     2581 gaagcccggc tcaccgaggc gaacacccag gaaatgctgc gcgaagtgcg cctcgcgctg
     2641 ctcgaggcgg acgtggcgct gcccgtcgtg cgcgagttca tcgccaaggt gaaggaaaaa
     2701 gccctcggcg aggaagtcat cagcagccta tcgccgggtc aggcgctggt cggtgtggtg
     2761 cagcgcgagc tgaccgcgat catcggcggc gactatgaag gcaaggcggt cgaactgaat
     2821 ctcgccgtca cgccgccggc cgtcatcctg atggcgggtc tgcaaggcgc cggcaagacc
     2881 accacggtcg gcaagctcgc caagcttcta cgcgagaagt acaagaagaa ggtcctcacc
     2941 gtttcgtgcg acgtttaccg accggctgct atcgcccagt tgaagacggt gaccgagcag
     3001 gtgggcgcgg acttcttccc gtcggaaccg gatcagaagc cggtggatat cgcgcgtgcc
     3061 gcggtggatt gggccaagcg ccattaccac gacgtgctgc tggtcgacac ggccggtcgc
     3121 ctcggtatcg acgaagcgat gatgcaggaa atcgccgcgt tgcacagcga gctgaaaccg
     3181 gcggaaacgc tgttcgtcgt cgacgcgatg ctgggtcagg acgcggtcaa caccgcgaag
     3241 gcctttagcg acgcattgcc gcttaccggg gtggtcctca ccaagctcga cggcgactcg
     3301 cgcggtggcg cggcactctc cgtgcgtcac gtgacgggca agccgatcaa gttcgtcggc
     3361 gtcgccgaaa agctcgacgg cctcgaaatt ttctatccgg atcgcatggc gaaccggatt
     3421 ctcggcatgg gcgacattct cgccctcgtc gaagaagcgc agcgcggtgt ggacgtacaa
     3481 gccgcgcaga agctcgccga caaggtcaag aaaggcggtg acttcgacct caacgatttc
     3541 cgcgcgcagc ttacgcagat gaagagcatg ggcggcctgt cgtcgttgat ggacaagctg
     3601 cccgcgcagt tccagcaggc cgcagccggc gccaacatgg gcatggccga atcgcagatg
     3661 cgccgcatgg aaggcatcat caactcaatg acgccgctcg agcgcgccaa gcccgagctg
     3721 atcaaggcca cccgcaagcg ccgcatcgcc gcgggcgcgg gcgtgcaggt gcaggaagtg
     3781 aaccgcatgc tgaatcaata cgaccagatg cgcacgatga tgaagaaact gaagggcggc
     3841 aacctgcaaa agatgatgcg cggcatgaag ggcatgatgc ccggcatgcg ctaagcttta
     3901 gcaagacggc ggcgcgcgtg ctttacatgc agtgcgggaa taatggcacg cgcgactgac
     3961 tccggagtgg tgcgggttta ttctgtaccg cacgccgccg ttctcaccca cactatgtat
     4021 acagttaccg tccgtcagac gtcatgaacc gcgaagaagc tctccacatt tttagccact
     4081 ccgaagaaat cgtttcggcc gacgacgtca acgcgtcgat cagcggcatg gctgccgcca
     4141 tccgcaacga gatgagcgag gactttccgc tcgtgctgtc ggtgatgggc ggcgcggcgg
     4201 tgttcaccgg catgctgctg ccgcacctcg attttccgct cgagttcgac tacatccacc
     4261 tgacccgcta tcgcaacacc accaagggcg gcaatgagat gcagtggcgg gtggcgccgg
     4321 ctgagtcggt gaaagatcgc gtcgtgctgg tgctcgacga catcctcgac gaaggcgaga
     4381 cgatggccgc gatccgcgac cgcatcctcg caatgggcgc gaagcgcttc ctgagcgcgg
     4441 tgctgtgcga gaagatcatt ccgaaggcga agccgttgcg tccggattac tgtggtttcg
     4501 aagtgccgga ccgctatgtg ttcggttgcg ggatggacgc caagggttac tggcggaatc
     4561 tgccgaccat tcgggcgttg accgaagggg cttaagcaag gtgttttagc ggcgcggttt
     4621 gcggcgcgca ttggcgtaaa aaaagcggct cgcgggccgc tttttgtttg tcgggcttct
     4681 gagagcgctt ctgcgttagc gtgttcgacc ccgcggcgaa tctcgctcaa gctttttgtc
     4741 cgtcaggctg gttgccacgc tcctgcgctg ccgcgttcaa ccccgcaccg aaactcactc
     4801 aaagttgatg cacaaaagaa cgaataccgc cgagcatcat ttcgactgag atcgccacca
     4861 gcaccaagcc catcagccgt tcgaaggcca tcatcgctcg ctcgccgagc cacgcctgaa
     4921 tgcgctccgc cagcatcaac acgatcgcgc agacgatcat cgtaacggtc aacgcaccga
     4981 tccactccaa catcttgccg ggcgcctgcg acgtcagcag catcaccgta gccagcgccg
     5041 acgggcccgc cagcgccgga atggcgagcg gcacgatgag cggctcaccg ccccgcgtgt
     5101 cgccgccgag cggaccgtct ggatgtggga acaccattct gagcgcgatc aggaacagca
     5161 cgatcccgcc gccgatgcgc aacgactggt cggtcagact catcatgcgc agaaagccgt
     5221 cgccgaccac catgaagacc agcaggatcg cgaacgcgat cgccacctca cggagaatga
     5281 cgatcgtgcg ccgcttcggc gatacacccc gcaggcaact gataaagagc ggaatgttgc
     5341 cgagcgggtc ggtaatcaga atcagcagga cggtcgcaga tacgaaggtg tactccaccg
     5401 tccgctccgc ttattgcgcc aaggcggcgc gaactttgcc gattaacgtt tgtgcggcgt
     5461 catcgaccgg cagcagcgtg gcttcaacat cgcgacggcc ctgatactcg agcttgccgt
     5521 ccttcaggcc acgatcgccg atcaccagac gatggggcac accgatcagc tcccagtcgg
     5581 cgaacatcac gcccggacgt tcgccgcgat cgtcgaggat cacgtcgacg cccgcttcga
     5641 gcaacgccgc gtacagcttg tcagcctgct cgcgcacggc ttcgctgcgg tcgtaaccca
     5701 tcgggcacag cacgacttcg aacggcgcga tcgagtccgg ccagatgatg cccttctcgt
     5761 cgaaattctg ttcgatcgcc gcgcccagaa tccgcgtgac gccgatgccg tagcagccca
     5821 tttccatctg gcgcggcttg ccggtctcgt cgagacaggt ggcgttcatc gcttccgaat
     5881 acttcgtgcc gagctggaac acgtggccga cttcgatgcc gcggcagatg tcgagcacac
     5941 ccttgccgtc cggcgacgga tcgcccttct tcacattgcg gatatcggcg acgatcggct
     6001 ccggcagatc gcggccccag ttcacgcccg tggtgtggta gtccacctca ttcgagccga
     6061 ccacgaagtc gctcatgttc gcgaccgtgc tatccgcgac gaccttgacc ggcttcttcg
     6121 tgttgatcgg gccgaggtaa cccggcggtg tgccgaaggt ctcgatgatc tcggcttccg
     6181 tggccatgcg gaagtcggcc aaacccggca gcttggctgc cttgatctcg ttcagatcgt
     6241 ggtcgcctcg cagcatcagc aaccagatgg tcggcgcggc gccttcgttt tccgtggcga
     6301 gtacgatcga cttgatcgtg cgctcgagcg gaatgttcag cagttcagcc acggcctcgc
     6361 acttcgcctt gcccggcgtt gcggtcttct tcatttcttc ggtcggcgcc gcgcgttgcg
     6421 cgtagagcgg cagcgcttca gccgcttcga cgttggccgc gaaatccgag gtcgggcaat
     6481 aggcgatcgc gtcttcgccg gtctcggcga tcacgtggaa ttcatgcgaa ccgctgccgc
     6541 cgatcgaacc gttgtccgcc gccaccgcgc ggaaatccag accaaggcgc gtgaaaatac
     6601 gcacgtacgc gtcgtacatc ttgcgatacg actcgcgcag accttcgatg tctttgtcga
     6661 acgagtacgc gtctttcatg atgaactcgc ggccgcgcat cacgccgaag cgcggacgga
     6721 tctcgtcgcg gaacttcgtc tggatctggt agaagttcac cggcaactgg cgatagctct
     6781 tgatctggcc gcgcgcaatg tccgtgatca cttcttcgtg ggttggtccg aacacgaaat
     6841 cggattgctt gcggtccttg aagcgcagca gctcggggcc gtatttttcc cagcggcccg
     6901 actcctgcca cagctcggcc ggctgcaccg acggcatcaa caactcgatg ccgcccgccc
     6961 ggttcatttc ttcgcgcacg atggcttcca ccttgcggat cgaacgcaga ccgatcggca
     7021 ggtagttata gatgccgcca gcaatgcgac ggatcatgcc ggcgcgcacc atgagcttgt
     7081 ggctgacgat ctcggcgtcg gcgggcgctt ctttcagcgt gccgataaag aaacgggaag
     7141 ctttcattca gattttccaa aagcggcggc tccggaggga ccgcccgaaa ggtgaaaacg
     7201 gtagggaaat cgacacagac ccggtttgga accggcacgg actcgccgaa cacccggccc
     7261 cgatccagcc tggaaccagc caaagtcttg accaggcccc cggcaaacgc cctgcacata
     7321 ccaggcaaaa ccccggcaaa actgacccat cgggccgctg aagccaccgc gcaaagcctt
     7381 tgcagcaagg gataaggcac ggaaagactg acaggctacc gcaaccgcgg gcgttttgcg
     7441 gcgaatcgtt ccaatctggt gcgaatcggt gcgtcgggtc cgttatctgt ttataatcaa
     7501 agcaatttta aaggattcga aggtggttgt atgctggatc gtgaaggctt tcgcccgaac
     7561 gtcggcatca tcctcttgaa cgcgcacaac gaggtgtttt ggggcaaacg gctccgtgaa
//
LOCUS       DATASH010000255         2667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5375949,
            whole genome shotgun sequence.
ACCESSION   DATASH010000255 DATASH010000000
VERSION     DATASH010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5375949"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1188
                     /gene="miaB"
                     /locus_tag="VHV99_07150"
     CDS             <1..1188
                     /gene="miaB"
                     /locus_tag="VHV99_07150"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014896118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEX3635251.1"
                     /translation="LGRVRELKEANPNLIIGVGGCVASQEGAAIVARAPYVDLVFGPQ
                     TLHRLPQMIDKRRASGRAQVDISFPEIEKFDHLPPARVDGPSAFVSIMEGCSKYCSYC
                     VVPYTRGEEVSRPLDDVLTEIAGLADQGVREVTLLGQNVNAYRAGITLGSSEIVDFAQ
                     LIEYVADIPGIERIRYTTSHPKEFTQRLIDTYAKVPKLVSHLHLPVQHGSDRILMAMK
                     RGYTVLEYKSVIRKLRAIRPDLSLSTDMIVGFPGETEEDFDKMMALIHEMKYDTSFSF
                     IYSPRPGTPAANLHDDTPREVKLKRLQHLQATIEENVQRISDSMVGKVERILVERPAR
                     KDPNELAGRTENNRVVNFPAPVASHARLIGQMVDVKIVHAYPHSLRGELVLVHDDAPA
                     TTH"
     gene            1235..2308
                     /locus_tag="VHV99_07155"
     CDS             1235..2308
                     /locus_tag="VHV99_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX3635252.1"
                     /translation="MKTTQQHLEFTAPRDDNARLANLCGPLDENLRQIEQALDVTLQR
                     RGHRITIRGRGAKLALTALEKFYNNSQEPLSVDDIQLALVEVRHPARHRANGNGNGDE
                     AGDPRFPGNPDHPFDQPADSGDDDLEELGPKLYTRRADLRGRTPAQREYLKQIIAHDV
                     TFGVGPAGTGKTYLAVACAVDALERDQVKRIVLTRPAVEAGERLGFLPGDLAQKVDPY
                     LRPLYDALYDLLGFDKTAKMFERQMIEIAPLAYMRGRTLNHAFIILDEAQNTTPEQMK
                     MFLTRIGFGSKAVVTGDTTQVDLPRGAKSGLIEAQQVLSDVRGIALTRFTSADVVRHP
                     LVARIVEAYDAHSLKTAGPADSR"
     gene            2408..>2667
                     /gene="ybeY"
                     /locus_tag="VHV99_07160"
     CDS             2408..>2667
                     /gene="ybeY"
                     /locus_tag="VHV99_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX3635253.1"
                     /translation="MSRTPKLTLNLQFPAAKAWPEHKALLPRATVAGWIKAALFADGE
                     LTVRFVDAEEGRTLNRTYRGKDYSTNVLTFAYAESEDDPVTG"
BASE COUNT          546 a          879 c          824 g          418 t
ORIGIN      
        1 cttggccgcg tgcgtgagct gaaggaagcg aatcccaatc tgatcatcgg cgtgggcggt
       61 tgcgtggcga gccaggaagg cgcggcgatc gtcgcgcgcg caccgtacgt cgatctggtg
      121 ttcggtccgc aaacgctgca ccgtctgccg caaatgatcg acaagcgccg cgcaagcggc
      181 cgcgcgcaag tggacatctc gttcccggaa atcgaaaagt tcgaccacct gccgccggcg
      241 cgcgtcgacg gcccgagcgc gttcgtgtcg atcatggaag gctgcagcaa gtactgcagc
      301 tactgcgtcg tgccgtacac gcgcggcgaa gaagtgtcgc ggccgctcga cgacgtgctg
      361 accgaaatcg ccggcctcgc cgaccagggc gtgcgtgaag tcacgctgct cggccagaac
      421 gtgaacgctt accgtgccgg tatcacgctc ggctcgagcg aaatcgtcga cttcgcccag
      481 ctgatcgaat atgtcgccga cattccgggc atcgaacgga ttcgctacac cacgtcgcat
      541 ccgaaggaat tcacgcagcg cctgatcgac acctacgcca aggtgccgaa gctcgtgagc
      601 cacctgcatc tgcccgtaca gcacggctcc gaccgcatcc tgatggcaat gaagcgcggc
      661 tacacggtgc tcgaatacaa gtcggtgatc cgcaagctgc gcgcgatccg tccggatctg
      721 tctttgtcca cggatatgat cgtcggcttc cccggcgaga cggaggaaga cttcgacaag
      781 atgatggcgt tgatccacga gatgaagtac gacaccagct tctcgttcat ctacagcccg
      841 cgtcccggca cgcccgccgc gaatctgcac gacgacacgc cgcgtgaagt gaagctcaaa
      901 cgcttgcaac atctgcaggc cacgatcgaa gaaaacgtgc agcgcatcag cgattcgatg
      961 gtcggcaagg tcgagcggat tctggtcgaa cgcccggcgc gcaaggaccc gaacgaactc
     1021 gcgggccgca ccgagaacaa ccgcgtggtg aatttcccgg cgccggtcgc gtcgcacgcg
     1081 cggctgatcg ggcagatggt ggacgtgaaa atcgtccatg cgtacccaca ttcgttacgt
     1141 ggcgagctcg tgctggtgca cgacgacgcg cccgcaacca cccactgagc gacgccagcc
     1201 agcgtcacga acccgacagg atcgatccac cgccttgaag accactcagc agcacctgga
     1261 attcaccgca ccgcgcgacg acaacgcgcg gctcgccaac ctctgcggac cgctcgacga
     1321 aaatctgcgg cagatcgagc aggcgctcga cgtcacgctg caacgccgtg gccaccgcat
     1381 cacgattcgc gggcgcggtg cgaaactcgc gctcaccgcg ctcgaaaagt tctacaacaa
     1441 ctcgcaggag ccgctctcgg tcgacgacat ccagctcgcg ctcgttgaag tgcgccaccc
     1501 ggcgcgccat cgcgcgaacg gcaacggcaa tggcgacgaa gccggcgacc cgcgctttcc
     1561 gggcaatccg gatcatccgt tcgaccaacc cgccgacagc ggcgacgacg acctcgagga
     1621 actcggcccg aagctgtaca cgcggcgcgc cgatctgcgc ggccgtacgc cggcgcaacg
     1681 cgagtatctg aagcagatca tcgcgcacga cgtgaccttc ggcgtcggtc cggcgggtac
     1741 cggcaagacg tatctcgcgg tagcctgcgc ggtggacgcg ctggaacgcg atcaggtcaa
     1801 acgcatcgtg ctgacgcggc cggccgtcga agcgggcgaa cgcctcggct tcctgccagg
     1861 cgatctggcg cagaaagtcg atccgtatct gcgaccgctc tacgacgcgc tgtacgacct
     1921 gctcggcttc gacaagaccg ccaagatgtt cgagcggcag atgatcgaaa tcgcgccgct
     1981 cgcctacatg cggggccgca cgctgaatca cgcgttcatc atcctcgacg aggcgcagaa
     2041 caccacgccc gaacagatga agatgttcct cacccggatc ggtttcggct cgaaggcggt
     2101 cgtcaccggc gatacgacgc aggtcgactt gccgcgtggc gcgaagagcg ggctgattga
     2161 agcgcagcag gtgctgtcgg acgtgcgcgg aattgcgctc acgcgcttca ccagtgcgga
     2221 cgtggtgcgg catccgctgg tcgcgcgaat tgtggaggcg tacgatgcgc attcgctgaa
     2281 aaccgccggt ccggcagact cccgctaatt tgcggcactg attttgcggc gccggttggc
     2341 ggcgcaaaat cagcgttgaa tcccacggga ttaggctgca gcaaaccagg caacacatcg
     2401 attacgcatg agccgcactc cgaaactgac attgaatctg caattccccg ccgccaaagc
     2461 ctggccggaa cacaaggcgc tgctgccacg cgccaccgtg gccggctgga tcaaggcggc
     2521 attgttcgca gacggcgaac tgaccgtgcg tttcgtcgac gccgaagaag gccgcacgct
     2581 gaaccgcacc tatcgcggca aggattattc gaccaacgtg ctgactttcg cgtacgccga
     2641 atcggaagac gatccggtga cgggcga
//
LOCUS       DATASH010000256         4702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3397272,
            whole genome shotgun sequence.
ACCESSION   DATASH010000256 DATASH010000000
VERSION     DATASH010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3397272"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..143
                     /locus_tag="VHV99_07165"
     CDS             <1..143
                     /locus_tag="VHV99_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011351416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX3635254.1"
                     /translation="LDMEQDDYHRAQTVAQSKTLMRFLLNTYLGGTPLATRQILIDLQ
                     NL"
     gene            140..904
                     /gene="pdxJ"
                     /locus_tag="VHV99_07170"
     CDS             140..904
                     /gene="pdxJ"
                     /locus_tag="VHV99_07170"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEX3635255.1"
                     /translation="MSFFLTSPNVIDLGVYIDHVATLRNARGTAYPDPIRAALMAEEA
                     GADVITLHLREDRRHIVDADVRTLRPLLKTRMNLECAVTQEMLDIACEVQPHDVCLVP
                     EKRQELTTEGGLDVAGQFAAVRAACKQLAEANSRVSLFIDPDETQIRAAYEAGAPVIE
                     LHTGRYAEAHDPAEQQREYERVVRAVEFGATLGIKVNAGHGLHYTNVQQIAAIEGIVE
                     LNIGHAIVAHAIFAGWDNAVREMKAIMVAARLGARA"
     gene            921..1364
                     /gene="acpS"
                     /locus_tag="VHV99_07175"
     CDS             921..1364
                     /gene="acpS"
                     /locus_tag="VHV99_07175"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEX3635256.1"
                     /translation="MTIYGIGTDVVQVSRVAAVMTRTNGRFAEKVLGPDELRVYHARH
                     ARSAARGLAFLATRFSAKEAFSKAIGLGMRWPMTWRALQTLNKPSGEPMVVASGELAE
                     WLDARGITARVTISDERDYAVSFVIAETGEAARGSDAPHAPASDE"
     gene            1432..2460
                     /gene="nagZ"
                     /locus_tag="VHV99_07180"
     CDS             1432..2460
                     /gene="nagZ"
                     /locus_tag="VHV99_07180"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEX3635257.1"
                     /translation="MKTTPGPVMLDVVGKTLNDDDRRRLAHPMTGGVILFARHYESRA
                     QLIALTDSIRAIRGDLLIAVDHEGGRVQRFRTDGFTVLPAMGKLGALWDSDVLHATKV
                     TTAVGYILAAELRACGIDMSFTPVLDLNYGQSQVIGDRSFHRDPRVVTLLAKCLNHGL
                     ALAGMSNCGKHFPGHGFAQADSHVAMPVDDRSLDEILRDDVAPYDWLGVSLGAVLPAH
                     VVYPQVDSKPAGFSRVWLQDILRKKLRFEGAVFSDDLSMEAARQGGTLTEGASAALQA
                     GCDMVLICNQPDEAEKVLDALRFTPSKESQQRLKRMRPRGKALKWSKLVADPQYLQAQ
                     ALLRSAFA"
     gene            complement(2582..3964)
                     /locus_tag="VHV99_07185"
     CDS             complement(2582..3964)
                     /locus_tag="VHV99_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006401253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX3635258.1"
                     /translation="MPHALIVEDDPNSLSGLSAILAADGFSVDTATTIAEARAALTRF
                     IPDVVLVDLNLPDGSGLDVLQHLPAHPPGGALPVIVMTGNATVESAIEGLRHGIWDYL
                     LKPVNIPRLRSLLARIPRPYELTEEVQTLRASLRQLGRFGSILGRSGTMQHVYDTIEH
                     VAPTEAAVLISGEAGTGKQFAAHTLHDMSRRRKGPFVTFDCRTAGSVAAHRSLDSVLF
                     GHERGAFSGAEQREPGLFEQASCGTLFIAEIAELPRPQQEALLRSLDSQTFMRVGGTN
                     HVATDFRLIASTRKNPRAAVADGSLHEDLALRLEAAALTLPPLRERGEDAALIAQAVV
                     DELNREVTARGMSDVAKQVGPNFLRECLAYEWPGNVRELQDRVRRAYHASGDVLESLR
                     ADEAGSANGRDLNGSRVQVTVGTPLADVEEMLIRATLDAVGGTRHRAASLLGISPKTL
                     YNKLQRMRLN"
     gene            complement(4286..4492)
                     /locus_tag="VHV99_07190"
     CDS             complement(4286..4492)
                     /locus_tag="VHV99_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEX3635259.1"
                     /translation="MNNDIAEGKWKQMVGKAKAAWGELTDDELTKAEGHADRLAGLIQ
                     ERYGKTREQAELEVRRFFDSNRHF"
BASE COUNT          855 a         1492 c         1503 g          852 t
ORIGIN      
        1 tgctcgatat ggaacaggac gattaccatc gagcgcagac cgtggcgcaa agcaaaacgc
       61 tgatgcgctt cctgctcaac acttaccttg gcggcacgcc actcgcgacg cgccagatcc
      121 tgatcgactt gcagaactta tgagcttctt tcttacgtcg ccgaatgtga ttgacctggg
      181 cgtgtacatc gatcacgtcg ccacgctgcg caacgcgcgc ggcaccgctt accccgatcc
      241 gatccgcgcc gcgctgatgg ccgaagaggc cggcgctgac gtgatcacgc tgcatttgcg
      301 tgaagaccgc cggcatatcg tcgatgccga cgtgcgcaca cttcggccgc tgttgaaaac
      361 gcgcatgaat ctggagtgcg cggtcacgca ggagatgctc gacatcgcgt gcgaggtgca
      421 gccgcacgac gtttgcctcg tgccggaaaa gcgtcaggaa ctgaccactg aaggcggcct
      481 cgacgtcgcc ggtcagttcg cggccgtgcg cgccgcatgc aagcagctcg ccgaagcgaa
      541 ctcgcgcgtg tcgctcttca tcgatccgga cgaaacgcaa atccgcgccg cgtacgaggc
      601 gggcgcgccg gtgatcgaac tgcacacggg ccgctatgcc gaagcgcacg atcccgccga
      661 gcagcagcgc gaatacgagc gcgtggtgcg tgccgtcgag ttcggcgcga cgcttggcat
      721 caaggtcaat gcgggtcatg gcttgcacta caccaacgtt cagcagatcg ccgcgatcga
      781 gggcatcgtc gaactgaata tcggccatgc gattgtcgcg catgcgatct tcgccggctg
      841 ggataacgca gtgcgcgaga tgaaggcgat catggtcgcc gcccgtctcg gcgcacgcgc
      901 gtaacgccgc ggcatcgcac atgacgatct acggcatcgg cacggacgtc gttcaggtca
      961 gccgcgtggc cgcggtgatg acgcgcacca acgggcgctt cgccgagaag gtgctcggtc
     1021 cggacgagct acgcgtttat cacgctcgcc acgcgcgctc ggcggcgcgc ggtctcgcct
     1081 ttctggcgac gcggttctcc gctaaagaag cgttctcgaa ggcgatcggc ctcggcatgc
     1141 gctggccgat gacctggcgc gcgctgcaaa cactcaacaa gccgagcggc gagcccatgg
     1201 tggttgcatc gggcgagctc gctgaatggc tcgacgcgcg cggcatcacg gcgcgcgtga
     1261 cgatcagcga cgaacgcgac tatgccgtgt cgttcgtgat cgccgaaacg ggcgaagcgg
     1321 cgcgagggtc ggacgccccg catgcgccgg ccagcgacga ataaatgata tgaacgcggc
     1381 gcacgtcggc cacgtctctc tttccctcag gcttttttcc agcggaattc gatgaaaacc
     1441 actcccggac cggtcatgct cgacgtagtc ggcaaaacgc tgaatgacga cgatagacgc
     1501 cgccttgccc atccgatgac cggcggcgtg attctgttcg cacgtcatta cgagagccgc
     1561 gcgcaattga tcgcgctgac cgattcgatc cgcgcgattc gcggcgatct gctgattgcc
     1621 gtcgatcacg aaggcggccg cgtgcagcgc tttcgcaccg acggcttcac ggtgctcccg
     1681 gccatgggca agctcggcgc gctgtgggat agcgacgtgt tgcatgccac caaggtgacg
     1741 accgcagtcg gctatatcct cgcggccgaa ttgcgcgcgt gcggcatcga tatgagtttc
     1801 acgccggtgc tcgatctgaa ttacggccag tcgcaggtga tcggcgatcg ctcgttccac
     1861 cgcgatccgc gcgtcgtgac cttgctggcg aagtgcctca atcacggtct cgcgctggcc
     1921 ggcatgagca attgcggcaa gcatttcccg gggcacggtt tcgcgcaggc agactcgcac
     1981 gtcgccatgc cggtcgacga ccgttcgctc gacgagatcc tgcgcgatga cgtggcgccg
     2041 tacgattggc tcggtgtgtc gctcggggca gtgctgcccg cacatgtggt ttatccgcag
     2101 gtcgactcga agccggcggg cttctcgcgt gtctggctgc aggacatcct gcgcaaaaaa
     2161 ttgcgctttg aaggcgcggt cttcagcgac gatctctcga tggaagccgc gcgccagggc
     2221 ggcacgttga ccgaaggcgc gagcgccgca ctgcaagccg gttgcgacat ggtgctgatc
     2281 tgcaatcagc cggatgaagc ggagaaggtg ctggacgcgc tgcgtttcac accgtcgaag
     2341 gaatcgcagc agcggcttaa gcgcatgcgg ccgcgcggca aggcgctgaa gtggagcaag
     2401 ctggtagcgg acccgcagta tctgcaagcg caggcgttgc tgcgcagcgc gtttgcatga
     2461 gcggcggata gttcgcggat caactgtttc aagcgtttgc attgaacgaa aaacggcgcc
     2521 tcactatcag gcgccgtttt ccattcaagc caggcctcac gcgaaagccg gcctcactca
     2581 ctcagttcaa ccgcatccgc tgcagcttgt tgtacagcgt cttcggactg atccccagca
     2641 gcgatgccgc gcgatggcgc gtgccgccga cagcatccag cgtcgcgcga atcaacattt
     2701 cctcgacgtc cgcgagcggt gtgccgacgg tgacctgcac gcggctgccg ttcagatcgc
     2761 gcccgttcgc cgagcctgct tcatcggcac gcaacgattc cagcacgtcg cccgatgcgt
     2821 gataggcccg gcgcacgcga tcctgcaact cgcgcacatt gcccggccac tcgtacgcaa
     2881 gacactcgcg caggaagttc ggtccgacct gcttggcgac gtccgacata ccgcgcgcgg
     2941 tgacttcgcg attcaactcg tcgacgacgg cttgcgcgat cagcgccgca tcctcgccgc
     3001 gctcgcgcag cggcggcagc gttaaagcgg ccgcttcgag acgcagcgcg aggtcttcgt
     3061 gcaggctgcc gtcggcgacc gccgcgcgcg gatttttgcg cgtggaggcg atcagccgga
     3121 agtcggtcgc cacatgattc gtgccgccaa cccgcatgaa ggtctgcgaa tcgagtgagc
     3181 gcagcagcgc ttcctgctgg gggcgcggca gttcggcgat ttccgcgatg aacagcgtac
     3241 cgcagctggc ctgctcgaac agacccggct cgcgttgctc ggcgccgctg aacgcgccgc
     3301 gctcgtggcc gaacagcacg ctatcgagcg agcgatgcgc cgcgacgcta cccgccgtgc
     3361 ggcaatcgaa cgtgacgaac ggccctttgc ggcgccggct catgtcgtgc agcgtgtgcg
     3421 cggcgaattg cttgcccgtg ccggcttcgc cggaaatcag cacggcggct tcggtcggcg
     3481 cgacgtgttc gatcgtgtcg tacacatgct gcatcgtgcc gctgcggccc agtatcgagc
     3541 cgaaacggcc aagttgacgc agcgacgcgc gcagcgtctg gacctcttcg gtcaattcgt
     3601 atggacgcgg aatgcgcgcg agcaagctac gcaaacgcgg gatgttgacc ggtttgagca
     3661 ggtagtccca gatgccgtgg cgcagaccct cgatcgcgct ttcgaccgtc gcattgcctg
     3721 tcatcacgat cacgggcagg gcgccgcccg gcggatgcgc cggcaagtgc tgcaacacat
     3781 cgagcccgct gccgtccggc agattcagat cgaccagcac gacatcgggg ataaagcggg
     3841 tcagcgccgc ccgggcctcg gcgatggtgg tcgcggtgtc gacggagaaa ccgtcggcag
     3901 cgaggatggc ggacaaacct gacaggctat tgggatcgtc ttcaacaatc agggcgtggg
     3961 gcatggcgag cgctattttt tagattgacg gaatgcccgg tatgccgcgc cgaatacgct
     4021 ggcgcgccat ccgggcgcga tggctggccg gtactgccgg tcgaataccg caccgcaccg
     4081 cgcgggcagc agcgtctccc acgactgcgg agcagccgct gccaagcagt tggcatgggc
     4141 gcgacgctgg tcgcggaagg cgacgtgcaa gcggatctcg ggcgactcga ctcacgtagt
     4201 tctggtcctt tgcgtttcaa tggatgcccg gcttgacgcg ctgtggcgcc tggggggcac
     4261 atctgtatat ccttcattcg accggttaga aatgccggtt gctatcaaag aagcgccgta
     4321 cctcaagttc cgcctgctcg cgggtcttgc cgtaacgctc ctggatcagc cccgccagtc
     4381 tgtcggcgtg gccttccgcc ttggttagct cgtcgtccgt caattcaccc catgctgcct
     4441 tggccttgcc aaccatttgc ttccacttgc cttcagcgat gtcgttattc atatcgcatc
     4501 tccggagttg atgaaataaa gccgtgtcgg gcctatagcc aagagaagca gggactgtgc
     4561 caaagccaat tacttaagtt tatcaatggt ttgtcgaacg tgaggcagga agaagacggg
     4621 caacggactg gcattttcga gacgcctgcc ggtaaagttt tcctcaacca tacacggcgg
     4681 cgcaaaaaag aaaaagcgcc cg
//
LOCUS       DATASH010000257         4120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6957908,
            whole genome shotgun sequence.
ACCESSION   DATASH010000257 DATASH010000000
VERSION     DATASH010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6957908"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VHV99_07195"
     CDS             <1..299
                     /locus_tag="VHV99_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012338500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HEX3635260.1"
                     /translation="RGRLTIADALTLGGGILDTDANPRQIFVMRGMREHPTTPDVYRL
                     DMTQPDAIMLSSQFQLQPMDIVYVGTAASTTFNRVLQQVLPSVQTLFYLKELTR"
     gene            510..2708
                     /locus_tag="VHV99_07200"
     CDS             510..2708
                     /locus_tag="VHV99_07200"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEX3635261.1"
                     /translation="MAGPIKTEEEDVVLGQLVQVILDDIWWLIGIAALIVGLAAAYCF
                     LAKPIYSADAHVRVEQSDNTSQALTQTQTGAAITSGSTSLPTDAEIEIIKSRGVVGPV
                     VQQLKLNFSVAPKTLPLLGSIAARMATPGQPAPPWLGLSSYAWGGEDAEVDSIDVVPA
                     LEGEKLTMKVFDEQHYEIYAKNGALLLRGQVGQQEQGGGVTILVSKLVARPGEEFTVT
                     RFNDLDAITAFQSAITVQEQGKQTGVIQISLEDKSPEHAAEVANALAQSYVRQHVSNK
                     QADASKMLDFLQSEEPRLKADLERAEAALTAYQRQSGSINASDEAKVYLEGSVQYEQQ
                     IAGLRLQMAQLTERYGDDHPMLVAARQQMAELEAQRAKYADRFRDLPATEVKAVQLQR
                     DAKVAEDIYVLLLNRVQELSVQKAGTGGNVHIVDQAMRPGAPVKPKKVLILSAAVILG
                     LICGTGFVFLRRNLFKGIDDPDHIERAFHLPVYGLVPQSAEQALLESGFERGGERLRS
                     VLANARPKDVTIESLRSLRTSMQFTMMDAKNRIVMLTGPMAGVGKSFLTVNLAVLLAN
                     SGKRVLMIDGDMRRGVLERYAGGVQENGLSELLSGQISLEEAIRATNVESLSFISCGR
                     RPPNPSELLMSPRLPQYLDGLARRYDVILIDTPPVLAVTDASIIGAYAGSTFFVMRSG
                     VHNEGEIADALKRLRAAGVHVQGGIFNGMPARSRGGYDRSYAATQEYLST"
     gene            2763..3695
                     /locus_tag="VHV99_07205"
     CDS             2763..3695
                     /locus_tag="VHV99_07205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3635262.1"
                     /translation="MKVTVLVPTYRRPADLARCLAALQRQSRAPDEVVVVARADDEAT
                     HACLRDPAVPGGLPLCVAPVDVPGQVAALNRGLDAANGDVIAITDDDAAPHVDWVERI
                     AAAFENDARLGALGGRDWVHEKGRVLDGERPLVGKVTAHGKIIGNHHLGVGGAREVDI
                     LKGANMSYRREAVRSIRFDQRLRGAGAQVHNDMAFSLAVKNAGWKLMYDPRVAVDHFP
                     AERFDDDRRDAQTMAALRNAAFNFHLILRDQLPPLRRETAWWWWTLVGTRVYPGLTHA
                     ALALVSKQASLRLSRWRAVRTGAHEARRALSRTA"
     gene            3859..>4120
                     /locus_tag="VHV99_07210"
     CDS             3859..>4120
                     /locus_tag="VHV99_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018431973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX3635263.1"
                     /translation="MSTKVHVHLFYGADPRFYRPGDNIGCLYGYHHAQSDALALTYSQ
                     DARESKPVKLLRRALKAALGFDFIHTWRNRAEMLRSDVIWTHT"
BASE COUNT          779 a         1314 c         1362 g          665 t
ORIGIN      
        1 tccgtgggcg cctgacgatc gccgacgcgc tcaccctggg gggcggcatt ctcgatacgg
       61 acgccaatcc gcgccagatc ttcgtgatgc gcggcatgcg cgagcatccg accacgccgg
      121 acgtgtaccg cctcgacatg actcagcccg acgcgatcat gctgtcgtcc cagttccagc
      181 tgcaaccgat ggacatcgtg tatgtgggca ccgcggcttc gaccaccttc aaccgtgtgt
      241 tgcagcaagt gttgccgagc gtgcagacgc tgttctacct gaaggagctg acgcgctaag
      301 cgtctgagcc gtcgatgggt cccgcgccgc tttcagaggg cgcgggatca gagaaaaacg
      361 ccaaagcaaa cgcaaacgcc aaagcaaaag caagcccaaa agcaaacgca aaacggcata
      421 cgccgaggca cacgccaacg cacatgccca agcccacgcc gaagcaaccg aaacgggatc
      481 gaattgtgag cactcaaatc tctccacaca tggctggtcc gatcaagacc gaagaagagg
      541 acgtcgtcct cgggcaactg gtgcaggtga tcctcgacga catctggtgg ctgatcggca
      601 tcgccgcgtt gatcgtcggg ctggccgccg cgtactgttt cctcgccaag ccgatctatt
      661 cggccgatgc gcacgtgcgg gtcgagcagt ccgacaacac ctcgcaggcg ctcacgcaga
      721 cgcagaccgg cgcggcgatc accagcggct cgacttcgct gccgactgac gctgaaatcg
      781 aaatcatcaa gagccgtggc gtggtcggcc cggtggtcca gcaactgaag ctgaacttca
      841 gcgtcgcgcc gaagacgctg ccgctgctcg gcagcatcgc cgcgcgcatg gcgacgccgg
      901 gccagccggc gccgccgtgg ctcggtttgt cgtcgtacgc gtggggcggt gaagatgccg
      961 aggtcgattc gatcgacgtg gtgcccgcgc tggaaggcga gaagctgacg atgaaggtgt
     1021 tcgacgagca gcactacgaa atctacgcaa agaacggcgc gctgctgttg cgcggccagg
     1081 tcggtcagca ggagcagggc ggcggcgtga cgatcctcgt cagcaagctg gtggcccgtc
     1141 cgggtgaaga attcaccgtg acgcgcttta acgacctcga tgcgatcacc gcgttccagt
     1201 cggcgatcac cgtgcaggag cagggcaagc agaccggcgt gatccagatc tcgctggagg
     1261 acaagagccc tgaacacgca gccgaagtgg ccaacgcgct cgcgcagtcg tatgtgcgtc
     1321 agcacgtgtc gaacaagcag gccgacgcga gcaagatgct cgacttcctg cagagcgaag
     1381 aaccgcgcct gaaggccgat ctcgaacggg ccgaagccgc gttgaccgcg tatcagcgcc
     1441 agtcgggctc gatcaatgcg agcgacgagg ccaaggtcta cctcgaaggc agcgtgcaat
     1501 acgagcagca gatcgccggt ttgcgtctgc agatggcgca actgaccgag cgttacggtg
     1561 acgaccatcc gatgctcgtg gccgcgcgcc agcagatggc cgaactggag gcgcaacgcg
     1621 ccaaatacgc cgaccgcttc cgcgatctgc cggccaccga agtgaaggcc gtgcaactgc
     1681 aacgcgacgc caaggtggcg gaagatattt acgtgctgct gctcaaccgc gtgcaggagt
     1741 tgtcggtgca gaaggccggt accggtggca acgtgcatat cgtcgaccag gcgatgcgcc
     1801 cgggcgcgcc ggtcaagccg aagaaggtgc tgatcctgtc ggcggcggtg attctgggcc
     1861 tgatctgcgg caccggtttc gtgttcctgc gccgcaatct gttcaagggg atcgacgacc
     1921 ccgaccatat cgagcgcgcg ttccatctgc cggtgtacgg tctcgtgccg cagagcgccg
     1981 aacaggcgtt gctcgaaagc ggcttcgagc gcggtggcga acgcctgcgc tcggtgctcg
     2041 cgaatgcgcg gccgaaggac gtcacgatcg aaagcctgcg cagcctgcgc acctcgatgc
     2101 agttcacgat gatggacgcg aagaatcgca tcgtcatgct gaccgggccg atggccggcg
     2161 tcggcaagag cttcctgacg gtcaatctgg cggtgctgct cgcgaactcg ggcaaacgcg
     2221 tgctgatgat cgacggcgat atgcgccgtg gcgtgctcga acggtatgca ggcggcgtgc
     2281 aggaaaacgg cttgtcggaa ttgctgagcg ggcagatctc gctcgaagaa gcgatccgcg
     2341 ccacgaacgt ggagagcctg agcttcatct cgtgcggccg ccgtccgccg aatccgtcgg
     2401 aactgctgat gtcgccgcgc ctgccgcaat acctcgacgg cctcgccagg cgctacgacg
     2461 tgatcctgat cgacacgccg ccggtgctgg cagtgaccga tgcgtcgatc atcggcgctt
     2521 atgccggttc caccttcttc gtgatgcgct cgggcgtgca caacgaaggc gagatcgcgg
     2581 atgcgttgaa gcgcttgcgt gcagccggcg tgcacgtgca gggcggcatc ttcaacggca
     2641 tgccggcgcg ctcgcgcggc ggttacgacc gcagctatgc agcgacccag gaatacctga
     2701 gcacctgagg ctccgcacat gattctgaat ccagcgcaaa cacgataaac aaggacatca
     2761 cgatgaaagt gacggtactg gttccgactt accgccgccc ggccgacctg gcgcgctgcc
     2821 tcgcggcgct gcagcggcag tcgcgcgcgc cggacgaagt ggtggtggtc gcgcgcgccg
     2881 acgacgaagc gacccacgcc tgcctgcgcg atccggcggt accgggcgga ttgccgctgt
     2941 gcgtcgcgcc ggtcgacgtg ccgggtcagg tcgcggccct caatcgcgga ctggatgcag
     3001 caaacggcga cgtgatcgcg atcaccgacg acgacgccgc gccgcacgtc gactgggtcg
     3061 agcgtatcgc tgccgcgttc gaaaacgatg cgcgcttagg cgcactgggc gggcgcgact
     3121 gggtgcatga aaaaggccgc gtgctggacg gcgagcggcc gctggtcggc aaggtcacgg
     3181 cgcacggcaa gatcatcggc aatcatcatc tgggtgtggg cggtgcgcgc gaggtcgaca
     3241 tcctgaaggg cgcgaacatg agttaccggc gcgaggcggt tcgctcgatc cgtttcgatc
     3301 agcgcctgcg tggtgcgggc gcccaggtcc acaacgacat ggccttcagc ctcgccgtga
     3361 agaacgccgg ctggaagctg atgtacgacc cgcgggtagc cgtcgatcat ttccccgccg
     3421 aacgtttcga cgacgatcgg cgcgacgcgc aaaccatggc ggcgttgcgc aatgccgctt
     3481 tcaattttca tctgatcctg cgcgaccagt taccgccgct gcggcgtgaa accgcgtggt
     3541 ggtggtggac gctggtcggc acgcgcgtct atccggggct cacgcacgcc gcgctcgcac
     3601 tggtgtcgaa acaggcgagc ctgcggctgt cgcgctggcg cgcggtgcgc actggcgcgc
     3661 atgaagcgcg ccgcgcgctg tcgcggaccg catgagccgc atgagccgca tgagccgcat
     3721 gagccgcgtg aaccgtgcgc aacaccgcgg ctccaacgtg tcccgcgcaa cccaatcgtc
     3781 ctgcacgttc gtgcatcggc ataggcgcca gtccgcgcat gccgatgctc ctttccaccg
     3841 gcagagcgga gtgtctgcat gagtacgaaa gttcatgttc atctgtttta cggcgccgac
     3901 ccgcgctttt accggccggg cgacaacatc ggctgtctgt atggctatca ccacgcgcaa
     3961 tccgacgcgc tcgcgctgac ctattcgcag gacgcgcgcg aaagcaagcc ggtcaagctg
     4021 ctgcgccgcg cgctgaaggc ggcgctcggg ttcgacttca tccatacatg gcgcaaccgc
     4081 gccgagatgc tgcgctcgga cgtgatctgg acgcataccg
//
LOCUS       DATASH010000258         5009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_232381,
            whole genome shotgun sequence.
ACCESSION   DATASH010000258 DATASH010000000
VERSION     DATASH010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5009
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232381"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="VHV99_07215"
     CDS             complement(<1..872)
                     /locus_tag="VHV99_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX3635264.1"
                     /translation="MNTTRPLPGAEAVAQIKDGPDADADTLAALAHAWDDRRGLIGWL
                     SAIDHKTIARRFMVTTFVFFLLAGLMALVMRTQLARPQSHVVGPDMYDQLFTIHGTAM
                     MFLFAVPVMQAVAMYLIPLLIGARSVAFPRMNAYAYWVFLFGGFTLFIAFALGAGPRS
                     GWFSYVPLAGPDYSTGKSSDIWAQMITFSELSALLVAVILITTILKMRAPGMSLNRMP
                     LFAWATLVTQFMVLFAMPAVMLASTALILDRLVGTQFYNPSLGGDVLLWQHLFWFFGH
                     PEVYLIFIPALGFMS"
     gene            complement(869..1873)
                     /gene="coxB"
                     /locus_tag="VHV99_07220"
     CDS             complement(869..1873)
                     /gene="coxB"
                     /locus_tag="VHV99_07220"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018444417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX3635265.1"
                     /translation="MSRVAEVAAQPQQSALQPAGVQAAHIAELWNLSLVVCGIVFALV
                     LLATLVALVRNTRGAAAHPPATHAPDLISLTHPEPRTQRIVIGASVLSVMVLVGLIVA
                     DVLTDRALSKLPLDEPVRIDMVGAQWWWQASYRPEGGQPGFVTANELHVPVGRPVIVS
                     LQAADVIHSFWVPNLHGKKDMLPGIDTTIEFRADRAGAYRGQCAEYCGVEHALMAMLV
                     VAEPPERYTAWVARQAQPAAQSTDAVALHGRDVFERASCANCHTVRGTPAKGTPGPDL
                     THLMSRETIAAGVLANTPRNLAVWIRDPNALKPGTLMPAVPLSDADRLALVRWLTTLQ
                     "
     gene            complement(2645..4936)
                     /locus_tag="VHV99_07225"
     CDS             complement(2645..4936)
                     /locus_tag="VHV99_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013341515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HEX3635266.1"
                     /translation="MDKKRKTEAYPYSSGGWGSLKAVTKILSQEKVALKDSTVLLRQN
                     KPDGFMCVSCSWAKPADPHTFEFCESGAKATAWDTTSKRVTHDFFDVHPVSELLTWHD
                     HDLEEAGRLTEPLRYDVATDRYVPVAWHQAFEEIGRELNGMDPDRVVFYTSGRASLET
                     SYMLQLFARMYGTNNLPDSSNMCHESTSVGLKEAIGVGVGTIMLDDFEKTDLMFFFGQ
                     NVGTNSPRMLHPLQDARKRGVPIITFNPLREPGLISFANPQSPVQMLSPTETQISTQY
                     HQLKTGGDTAAILGLCKVVITADDAALAAGGPRVLDDAFIREHTLGFEDFANFARQAE
                     WTDIERVSGLSRADIERAADEYIKANAVIIHYGMGLTQHRMGVQNVRMLCNFLLLRGN
                     IGKPGAGPSPVRGHSNVQGQRTVGVTEKPELAPLDKLAQQFSFEPPRKKGMNIVEAFE
                     AMVKGEVSAVLNLGGNLVRSVPDRLQTEPAWSRLRLTVNVATKLNRSHLVHGAVSYIL
                     PCLSRIEIDRQASGDQAVSMEDSTGCMHGSHGVTEAAGTDVRSEPFIIAGIANATLAG
                     RSTVDWDAWCGDYSRVRAAIAETYPEIFHDFEARMWTPGGFPRPLPARERKWQTKSGK
                     AEFMVPESLGEDPDMAEREPGALRLMTLRADGQFNTTIYNLDDRFRGIKGSRMVILMN
                     RADMESHSLQEGDKVTLQTIADDGIQRRVEGLRIVPYDIPPGCIAGYYPECNPLLPLW
                     HYAKESKVPGAKSIPVRIIERHP"
BASE COUNT          901 a         1623 c         1577 g          908 t
ORIGIN      
        1 gacatgaagc cgagcgccgg aatgaagatc aggtagacct cgggatggcc gaaaaaccag
       61 aacaggtgct gccatagcag cacgtcgccc ccgagcgacg ggttatagaa ctgcgtgccc
      121 acaagtcgat cgagaatgag cgcagtgctc gccagcatga ccgcgggcat cgcgaagagc
      181 accatgaact gtgtgacgag cgtggcccac gcgaacagcg gcatccggtt cagcgacatg
      241 cccggcgcgc gcatcttgag gatggtcgtg atcagtatga ctgcgacgag caacgcggac
      301 agctcgctga acgtaatcat ctgcgcccag atatcgctgc tcttgccggt cgagtagtcg
      361 gggccagcaa gcggcacgta gctgaaccag ccggagcgtg gacccgcccc gagcgcgaag
      421 gcgatgaaga gcgtgaaacc gccgaacagg aagacccagt acgcgtatgc gttcatccgc
      481 ggaaacgcaa cgctgcgtgc gccgatcagc aacggaatca ggtacatcgc caccgcctgc
      541 attactggga cggcgaacag gaacatcatg gcggtgccgt ggatcgtgaa gagctggtcg
      601 tacatgtcgg ggccaaccac gtgactctga ggacgcgcga gctgagtgcg catcacaagt
      661 gccatgagtc ccgcgagcag aaagaacacg aacgtcgtga ccatgaagcg gcgcgcgatg
      721 gttttgtgat cgatcgcgct caaccagccg atcagcccgc ggcgatcgtc ccacgcgtgc
      781 gccagcgcgg ccagcgtatc ggcgtcggca tccggtccgt ccttgatttg cgcgacggct
      841 tccgcaccgg gtagcggtcg ggtcgtgttc attgcagtgt ggtaagccat cggacgagcg
      901 cgaggcgatc ggcatccgaa agcggcaccg ctggcatcag cgtgccaggc ttcagcgcat
      961 tcggatcgcg tatccacacg gcaaggtttc gaggagtgtt ggcaagcacg ccggcggcga
     1021 tcgtctcccg gctcatcaga tgcgtgaggt ccgggccggg ggtgcccttc gcgggcgtgc
     1081 cgcgcaccgt atggcaattc gcgcaactgg cgcgctcgaa cacgtcgcgg ccatgcagcg
     1141 cgacggcgtc ggtgctctgc gctgcgggct gcgcctgccg cgcgacccag gcggtatatc
     1201 gctcgggcgg ctcggcgacg acaagcatcg ccatcaacgc atgttccacg ccgcaatatt
     1261 cggcgcattg cccacgataa gcgcccgccc ggtccgcgcg aaactcgatc gtcgtgtcga
     1321 tgcccggcag catgtccttc ttgccgtgca ggttgggcac ccagaagctg tggattacat
     1381 cggccgcttg caacgacacg atgaccggac gaccaaccgg cacatgtaat tcgttcgcgg
     1441 tgacgaagcc gggttgtccg ccctccgggc gatacgacgc ctgccaccac cattgcgcgc
     1501 cgaccatatc gatgcgcaca ggctcgtcta gcggtagctt cgacagcgcc cgatccgtca
     1561 gcacgtcggc gacgatcagt ccgacgagga ccatgaccga cagcacgctg gcgccgatca
     1621 cgatgcgctg cgtgcgaggt tcgggatgcg tgagcgagat caggtcgggc gcgtgagtcg
     1681 ccggcggatg cgcagcggcg ccgcgcgtgt tgcgcaccag cgcgacgagg gtcgccagca
     1741 gcacgagtgc aaagacgatc ccgcacacga ccagcgaaag attccacagc tccgcgatgt
     1801 gcgccgcctg gacgcctgcg ggctgcaacg cgctttgctg tggctgcgcg gcgacctccg
     1861 ccacgcgcga catcgcccag agcgagccgg ctgcggtacg ggtcggcagt ttccagccgg
     1921 cccgcgcagc gccgccacgg caccgcccgg tctcgcgaat gtgggctcca aggtcggcga
     1981 tcatttacgt aggtgagcaa tcgagtgtgg agacctgtag cagtttgcgt tccgcatgct
     2041 cgtcggagcc ggcggccgcc gcgaaagcgc gccaggcgag gcagacttca tgtgccgagc
     2101 aggcggcgcg atcggcacct gcgcggccag aacggggcat tccggtgcgc ataccccact
     2161 gcgcacaccc cactgttgaa ggcgtcgacc attccacaat ctgggccagc aagcgcgggc
     2221 cggacgcacc ggtgtgaagg aagaccagta ctgcgtcggg aaatgtgccg gcaactgcgc
     2281 cgtcacctcg cgcggcgctt cccggccgcc acaggaaccg ccaatgacca ggacgcccgt
     2341 ccctcgccac tgtgccatcc atacgctgct ggcaatattc ttcgacccgc gtgcgtccgc
     2401 atcgccgtga ggtacgtggt gcagttgcgc cgccggcgcg gtccggccgt ccgctgatgc
     2461 tgcgccgacg ggtcgacgtg ccgccgcgat tgccctcaac gcctgaccgc cgtccctttc
     2521 gatctgctgg cgcgcctgct gccggcccct gcccgcctcg ccgtcccgcc accggtcgtt
     2581 ctccctgaag cgacactctc gcgtgcgttg ccggcttcgt gatttttgtc catgcaataa
     2641 cgcgctaggg atgccgctcg ataatgcgaa ccggtatcga cttcgcgccc ggcaccttgc
     2701 tttccttcgc gtaatgccaa agcggcagca gcggattgca ctccgggtag tatccggcaa
     2761 tgcagccagg cggaatgtcg tacggtacga tgcgcagccc ttcgacccgg cgctggatgc
     2821 cgtcgtcggc gatggtttgc agcgtcacct tgtcaccctc ctgaagcgaa tgcgattcca
     2881 tgtcggcacg attcatcagg atgaccatac gggatccctt gatgccacga aaccggtcgt
     2941 cgaggttgta aatggtggtg ttgaactggc cgtccgcgcg cagcgtcatc agccgcaacg
     3001 cgcccggctc acgctccgcc atgtccgggt cctcgcccag cgattcaggc accatgaatt
     3061 ccgccttgcc gcttttcgtc tgccatttgc gttcgcgcgc cggcaaaggc cgcgggaaac
     3121 cgccgggtgt ccacatcctc gcctcgaaat cgtgaaagat ctcggggtag gtctcggcga
     3181 ttgcggcgcg cacccggctg tagtcgccgc accatgcgtc ccagtcaacc gtcgaacgcc
     3241 ccgccagcgt cgcgttggcg atcccggcga tgatgaacgg ttccgagcgc acgtcggtgc
     3301 cagccgcttc agtcacgccg tgggagccgt gcatgcagcc ggtactgtct tccatcgaga
     3361 cagcctggtc tccgctcgcc tgccggtcga tctcgatgcg gctcagacac ggcaggatat
     3421 aagacacggc gccgtgcaca agatgactcc ggttgagctt ggtagcgaca ttgactgtga
     3481 ggcgcaggcg gctccatgcc ggctccgtct gcaaacggtc cggcaccgag cgcacgagat
     3541 tgccgccgag gttgagcacc gcgctcacct ctcctttcac catggcttcg aacgcctcga
     3601 caatgttcat ccctttcttg cggggcggct cgaatgagaa ctgctgcgcc agcttgtcga
     3661 gcggcgcgag ttctggcttc tccgtgacgc cgaccgtgcg ctgtccttgc acattggaat
     3721 ggccgcgcac tggcgaaggc cccgcgccgg gcttgccgat attgcctcgc agcagcagaa
     3781 aattacacag catgcgtaca ttctgcacac ccatacggtg ctgcgtgagt cccatgccgt
     3841 agtgaataat gacagcgttc gccttgatat attcatcagc cgcgcgctcg atgtcggcgc
     3901 gggataaccc gctcacccgc tcgatgtccg tccattcggc ctgccgcgca aagttcgcga
     3961 agtcctcaaa gccgagcgtg tgctcgcgga tgaacgcatc gtccagcacg cgcgggccgc
     4021 cggcggcgag tgcagcgtcg tctgcggtaa tcactacctt gcacagaccg aggatcgccg
     4081 cggtgtcgcc gccagtcttg agttggtgat attgcgtgct aatctgcgtc tcggtcggcg
     4141 agagcatctg cacgggcgac tgcggattcg cgaagctgat caggccgggc tcgcgcagag
     4201 gattgaaggt gatgatcggc acgccgcgct tgcgggcgtc ctgcagaggg tgcagcatcc
     4261 gggggctgtt cgtgccgacg ttttgcccga agaagaacat gagatcggtc ttttcgaagt
     4321 cgtccaacat gatggtgccg acaccgacgc cgatcgcctc tttcagtccg acgctggtgc
     4381 tttcgtgaca catgttcgaa ctgtccggca ggttgttcgt gccatacatt cgcgcgaaca
     4441 actgcaacat ataggacgtt tcgagcgacg cgcgaccgga ggtgtagaac acgacacgat
     4501 cagggtccat gccgttcagt tcgcggccga tctcctcgaa tgcctggtgc caggctaccg
     4561 gcacgtagcg atccgttgcc acgtcgtagc gcaacggctc ggtgaggcgc ccggcttcct
     4621 cgagatcatg gtcgtgccac gtcagaagct cgctcaccgg atggacgtcg aagaaatcgt
     4681 gcgtgacccg tttggacgtg gtgtcccatg ctgtcgcctt cgcgccgctc tcgcaaaatt
     4741 cgaaggtgtg cgggtcagcc ggcttcgccc atgagcaact gacgcacatg aatccgtccg
     4801 gtttgttctg acgcaacagc accgtgctgt ctttcagcgc aaccttctcc tgcgagagaa
     4861 ttttcgtgac cgccttcagc gatccccaac cgcccgaaga gtacggatac gcttcggtct
     4921 ttcttttctt gtccatggga tgttccttgc acaacgatga agttctgctc gtgttgtggc
     4981 aagcgcagca agcggcgttc caaacgtgg
//
LOCUS       DATASH010000259         7212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1024693,
            whole genome shotgun sequence.
ACCESSION   DATASH010000259 DATASH010000000
VERSION     DATASH010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7212
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1024693"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1340)
                     /locus_tag="VHV99_07230"
     CDS             complement(<1..1340)
                     /locus_tag="VHV99_07230"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HEX3635267.1"
                     /translation="MNRIVEPGNPFASTASVTETATRVSVQPVILAGGSGTRLWPLSR
                     ERNPKQLIGLMGDESLLEATLRRLDLAFATAGYGATAPGRPGGATPGTATPLVVCCED
                     LRLQTLERLEQCGRPARMLLEPVPRNTAPALTVAALAARAAAAAGDDPIIVALPADHL
                     IDDHAVFGAALAEAVSHAARGAIVTLGVPPQRAETGYGYIRTGLALGAHGARAIEGFV
                     EKPDAELAERYAASGEYWWNSGMFVVRASVWLAAIALCRPLIAAACAEAFEHATLDGG
                     GALHLARAAFAACPSDSIDYAVMERLATDARLVGVAVPLIAGWSDVGAWDAVWEASDK
                     DACGNVARGRVMFEDSNDSFAHSEGRLVACLGVSGVVVVETADAVLVAAKDRVQDVKA
                     IVSRLKLGHGTEAEEHRKVGRPWGFYDSLERGERFQVKRIVVKPGGRLSLQMHH"
     gene            complement(1427..2260)
                     /locus_tag="VHV99_07235"
     CDS             complement(1427..2260)
                     /locus_tag="VHV99_07235"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3635268.1"
                     /translation="MNSRSELDRINRMAWNSADATRVFTLDKTYSDPGEQAAFEWLAP
                     RCAGEPLLDIGVGTGRTIPLMTSISSDYTGVDYTLKLLEIAQSRYPQLDLRHMDARDM
                     SALPSGHYGLVQFSWNGIDCVDYDDRAKILAEMYRLVRPGGYVLFSSHNRGGPGYREN
                     IWQLLPQFSFNPLKLGWRTLRSMRRFQLGMLNYMRNVKLNHDYGGYAVKTAAAHNFGI
                     VIVYTTLQEQRRQLAQIGFQSSAVFGSCQGNRIADEVESSDTWWFHFIARKPVAACRF
                     D"
     gene            complement(2315..4276)
                     /locus_tag="VHV99_07240"
     CDS             complement(2315..4276)
                     /locus_tag="VHV99_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3635269.1"
                     /translation="MRPVLFDGRFGWLHPAEGSSGVVLCAPFGYDALCTYRGMRRLAE
                     RLAARGMPVLRFDYAGTGDAAGEASEPGRWRAWIDSIKLAVALLRATTGVERVTLCGL
                     RLGGMLAALAAQELGGVDGLVLLAPVLSGKNYQRELRAHYRKWLSMPAAMDCEAEPDT
                     DAFVEAYGFRLYGDTLASLRAVDLQCDAARPAERLLVLDSLDPARVDALVAHYREQGV
                     QVERQAFDEYGKFMMESLDSEIPQAAFRSIEDWLVSGAEHPATREHPIADSDAHAHAA
                     QDAEHMADRVVDRSSDHTTGHPTSHTTGHLVEPGVIETPVWLDGGRMFGIYCVPASHA
                     AAAPAALLLNTGAVSRVGNARLGVRFARRLARQGIASLRVDLGGLGDSQPSLDTLSLD
                     ALYAQAGADDAACAARWLTAQGHRGAVLLGICAGAFVGLHAASREPAVIGAVLVNLQK
                     FTWHNICDEHGKPAVPVAAFGSTRTYLRSMCQPRKWLRVVRGQTGGLPVARELAKRFA
                     ARFGATLADRIERISGIQLGPTDERRLFTALHARGVDTRLLYGVLDEGVEELERYFGA
                     QGGRLRRFERIGVAFCERTDHAILSSRAQENVMAYFEGFFHSTFSETRQPESSVAGGS
                     LRTRLDWRHARTMQWLLKRVRALRLSGEA"
     gene            complement(4435..5433)
                     /locus_tag="VHV99_07245"
     CDS             complement(4435..5433)
                     /locus_tag="VHV99_07245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX3635270.1"
                     /translation="MTQTTNEAGLTSGSGAAARLISICIPTCNRPVLLEQAIDSCHAQ
                     TYGEFEIVIGDDSSDDSARQVVERCRLRGTRPIPIRYERNLPRLGQAANVNRLFARAH
                     GARLVLLHDDDLLEPNALSCLDACWHAQPELTAAFGKQRVIADDGTPRPQATERLNHD
                     YLRVPERAGRLPVPALAGIDQMFPNNGYLVDTLAARRIAYRVAAEVGEACDFDFGLRL
                     SVDAVAICFVDEFVSVSRETALSISSHALPAMHAFAQLRASSVPAAAQAARDDALRRV
                     APRAASAYARAGEAAAAWRVLLSRHYPMRERLRARFFYHVLLATRSTFALRAPQGN"
     gene            5649..7106
                     /locus_tag="VHV99_07250"
     CDS             5649..7106
                     /locus_tag="VHV99_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX3635271.1"
                     /translation="MERSIVKNIFINFAGAIAPTFVSLVTVPAYIHLMGVERYGVINL
                     VWTLIGYFSVLDLGTSLATENQISKARAANDDSIDLIFWSAWFMNLGTGIVGGLLVYL
                     GTFLYITHGVKIEPAFQREVMASLPWIAVAVPIANVSWVFAGAINGVERFTSFNINQT
                     LGTALFQLLPLAAIFCFSPSLAVVIPAAVVARLLGGVLLGAAAFRAVGIRRVRMPQWR
                     VMAELFRYGRWMLMFSGANMIASTLDRVLVGALLGARFVTYYATPQNLITRLNLLPIA
                     MVRTLFPRLSAVSRADADALAHSSLAFLNGAFTPCVIVALFALKPFLTLWLGPTVAAA
                     APVASVLVLGVWLSGQAGILGILIQAQTNPAYIAGVSWLQLPVFAGALWCGIHWFGIM
                     GAGVVVVMKSLFDYAVLLFFARLHVGTIVRNMLAHLAFLVVALVLADSITALPSLIAA
                     AVVMAAANLGMSLHGSSELRSVVYKLWTRLTLSAS"
BASE COUNT         1264 a         2449 c         2252 g         1247 t
ORIGIN      
        1 tgatgatgca tctgcaacga cagccgccca cccggtttga cgacgatgcg cttcacctgg
       61 aagcgctcgc cacgttcgag cgaatcgtaa aagccccacg gccggccgac cttgcgatgc
      121 tcctcggcct ccgtgccgtg ccccagcttc agccggctga cgatcgcctt gacgtcctgc
      181 acgcgatctt tcgcggcgac cagcaccgcg tcggcggtct cgaccaccac cacgccgctc
      241 acgccgagac acgcgacgag ccggccctcg gaatgcgcga agctgtcgtt cgaatcctcg
      301 aacatcacgc ggccacgggc gacgttgccg caagcgtcct tatcggacgc ctcccatacc
      361 gcatcccagg cacccacgtc ggaccacccc gcgatcagcg gcacggcgac gccgacgaga
      421 cgcgcgtcgg tagcaagacg ctccatcacc gcgtaatcga tcgaatcgga aggacacgcg
      481 gcgaacgccg cgcgcgcgag atgaagcgcc ccgccgccat ccagcgtggc atgctcgaaa
      541 gcctccgcgc aggccgccgc gatcagaggc cggcatagcg cgatggccgc gagccagacc
      601 gacgcgcgca ccacgaacat gccgctgttc caccagtact cgccggaggc ggcgtagcgt
      661 tcagcgagct ccgcgtcggg tttctcgacg aagccttcga tcgcgcgtgc cccgtgcgcg
      721 ccaagcgcca gtcccgtgcg gatatagcca tagccggttt ccgcacgctg cggcggcacg
      781 ccgagcgtca cgatcgcacc gcgcgcggcg tgagaaaccg cttcagcgag cgccgcgccg
      841 aaaaccgcat gatcgtcaat caggtgatcc gcgggcaacg ccacgatgat tggatcgtcg
      901 ccggccgcgg ctgccgcgcg cgcggccaac gccgccacgg tcagcgccgg cgcggtattg
      961 cgaggcaccg gctcgagcag catgcgcgcc ggccggccgc actgttcgag acgttcgagc
     1021 gtttgcagcc gcaggtcctc gcaacacacc accagcggcg tggccgtgcc cggcgtagcg
     1081 ccgcccggcc tccctggcgc ggtcgcgcca taccccgccg tggcgaatgc aagatcgagc
     1141 cggcgcaagg ttgcttcgag cagcgattcg tcgcccatca ggccgatcag ttgtttggga
     1201 ttgcgttcgc gcgacaacgg ccagagccgc gtgccggagc cgcccgcgag aatcaccggc
     1261 tggacggaca cacgtgtggc cgtttcggtg acactcgcag tcgacgcgaa cggattgcct
     1321 ggttcaacga tacgattcat tgtgcgatcc ccgtaagtgt tcaagctggt ctgttatgca
     1381 ttctttatct gatgcgcgtg gtgcgccgcc ttgcacccgg cggcggtcaa tcgaaacggc
     1441 acgcggcaac cggtttgcgc gcgatgaagt gaaaccacca cgtgtcgctg ctttccacct
     1501 cgtcggcaat gcggttgccc tggcaactac cgaacaccgc gctgctctga aaaccgatct
     1561 gcgcgagttg gcggcgctgc tcctgcagcg tcgtatagac gatcacaatg ccgaagttgt
     1621 gcgccgccgc cgtcttcacc gcatagccgc cgtagtcgtg attcagcttg acgttacgca
     1681 tgtagttcaa catgccgagc tgaaagcgcc gcatcgagcg cagcgtgcgc cagccgagct
     1741 tgagcggatt gaagctgaat tgcggcagca attgccagat gttctcgcgg tagccggggc
     1801 cgccgcggtt gtgcgatgaa aacagcacat aaccgccggg ccgcaccagc cggtacatct
     1861 ctgcgagaat cttcgcgcga tcgtcgtagt ccacgcagtc gatgccgttc cagctgaact
     1921 gcaccagccc gtaatgtccg gacggcaatg ccgacatgtc gcgcgcatcc atatgccgca
     1981 agtcgagttg ggggtagcgc gactgcgcga tctccagcag tttcagcgtg taatcgacgc
     2041 cggtgtagtc gctcgagatc gacgtcatca gcgggatcgt gcgtcccgtg cccacgccga
     2101 tatccagcaa cggttcaccg gcgcagcgcg gcgcgagcca ctcgaacgcg gcctgctcgc
     2161 cggggtccga gtaggtttta tcgagcgtga agacgcgggt ggcatcggct gaattccatg
     2221 ccatacgatt gatgcggtcc aactcggatc gactgttcat gaccatgctc catcgaagtg
     2281 gaagcgcgcc ggaaacgaca acccgacgcg atcattacgc ttcccctgat agacgaagcg
     2341 cgcgcacacg ttttagaagc cactgcatgg tgcgtgcatg ccgccagtcg agccgcgtgc
     2401 ggagcgagcc gccggcaacc gatgactcgg gctgacgggt ttcgctgaaa gtgctatgga
     2461 aaaacccctc gaaatacgcc atcacgtttt cttgcgcgcg tgacgagaga atcgcgtgat
     2521 cggtgcgctc gcagaaggcc acgccaatcc gttcgaaacg ccgcagccgc ccaccctgcg
     2581 caccgaagta gcgttcgagc tcctcgacgc cctcatcgag cacgccgtac aggagacgcg
     2641 tgtcgacgcc tcgcgcatgc agcgcggtga agaggcgccg ctcgtccgtc ggccccagct
     2701 gaatgccgct gatgcgttcg atcctgtcgg cgagcgtcgc gccgaatctg gccgcaaagc
     2761 gcttcgccag ctcgcgtgcg actggcaggc cacccgtctg cccccgcacg acgcgaagcc
     2821 atttgcgcgg ctgacacatc gagcgcagat aggtacgcgt cgaaccgaaa gcggcgacgg
     2881 gcacggccgg cttgccgtgt tcatcgcaga tgttgtgcca cgtgaacttc tgcaaattga
     2941 ccagcacggc gccgatcacc gccggctcac gcgacgcggc atggaggccg acgaaggcgc
     3001 ccgcgcagat gccgagcagc accgcgccgc gatgtccttg cgccgtcagc caacgcgccg
     3061 cgcacgcggc gtcgtcggcg cctgcctgcg catacagcgc atcgagactc aacgtatcca
     3121 gcgacggctg gctgtcgccc aggccgccga gatcgacacg cagcgacgca ataccttgcc
     3181 gcgcaaggcg ccgggcgaag cgcacgccga gccgcgcatt gccgacccgc gacacggcgc
     3241 cggtattcag caacagcgca gcaggcgcgg ccgcagcgtg tgatgcgggc acgcaataga
     3301 tgccgaacat ccggccgccg tcgagccaca ccggcgtttc gatcacaccc ggttcgacga
     3361 ggtggccggt ggtgtggctg gtgggatggc cggtggtgtg atcagacgag cggtcaacaa
     3421 cgcgatccgc catatgctca gcatcttgcg cggcgtgagc gtgagcgtca ctgtccgcaa
     3481 tgggatgctc acgcgtggcc ggatgttcgg cgccactcac cagccaatcc tcaatcgacc
     3541 gaaacgccgc ctgcggaatc tcgctgtcga gcgactccat catgaacttg ccgtattcgt
     3601 cgaacgcctg ccgctccact tgcacgcctt gttcgcgata gtgagcaacg agcgcatcga
     3661 cccgcgcggg gtccagcgaa tcgagcacca gcagacgttc ggccggacgc gcggcatcgc
     3721 attgcagatc gactgcgcgc aagctcgcga gggtgtcgcc atagaggcga aaaccgtacg
     3781 cttcgacgaa tgcatcggtg tcaggctcag cttcgcaatc catcgcggcc ggcatcgaca
     3841 accacttgcg gtaatgcgcc cgcaactcgc gctgataatt cttgcccgac aaaacagggg
     3901 ccagcagcac cagcccatcc acgccgccga gttcctgcgc ggccagcgcc gcgagcatgc
     3961 cgccaagccg cagcccgcac agcgtcacgc gttcgacacc ggtcgttgca cgcagcagcg
     4021 ccacggccag cttgatgctg tcgatccacg cgcgccagcg gccgggctcg ctcgcctcgc
     4081 ccgccgcatc gccggtgccg gcgtagtcga accgcagtac cggcatgccg cgcgcggcga
     4141 gccgttcggc cagccgccgc atgccgcgat aggtgcacag tgcgtcgtac ccgaacgggg
     4201 cacacagcac cacgccactc gacccttctg ccggatgcaa ccagccgaac cggccatcga
     4261 acagtactgg cctcacacta agctcctcac cgggacaacg ccgctttcga tcactacaac
     4321 aaacgccgcc cggttcatct gtacgccctg ccacattagg tcggactccg atacgtacgt
     4381 ggtgtggcga cccacacgcc gtgacggccg agccgattca aatcgacgta tcgttcaatt
     4441 gccctgcggt gcgcgcaacg caaaggtcga ccgggtcgcg agcagcacgt gatagaaaaa
     4501 gcgcgcccgc agccgctcgc gcatcgggta gtgccgcgac agcagcacac gccaggcggc
     4561 ggccgcttca ccggcgcgcg cataagccga ggcggcgcgc ggcgcgacgc ggcgcagcgc
     4621 gtcatcgcgc gcggcctgcg cggccgcggg cacgctgctc gcgcgcaatt gcgcgaacgc
     4681 atgcatggcc ggcaacgcgt ggctcgatat cgacagcgcc gtctcgcgcg aaaccgacac
     4741 gaactcatcc acgaaacaga tcgcgacggc atcgacactg agccgcaagc cgaaatcgaa
     4801 atcgcatgct tcgccgactt cagcggccac gcggtacgca atccggcgcg cagccaacgt
     4861 atccaccaga taaccgttgt tagggaacat ctgatcgatg cccgccagcg ccggcaccgg
     4921 cagccggccg gcgcgttcag gcacacgcag ataatcgtga ttcaggcgtt cggtggcttg
     4981 cggccgcggc gtgccatcgt cggcgatgac gcgttgtttg ccgaacgcgg cggtcaactc
     5041 gggctgcgcg tgccagcatg cgtcgagaca gctcagcgcg ttcggctcga gcagatcgtc
     5101 gtcgtgcagc agcacgagcc gcgcgccgtg ggcgcgcgcg aacaaccggt tcacgttcgc
     5161 cgcctgtcca agccgcggta gattgcgctc atagcggatc gggatcggcc gcgtgccgcg
     5221 caagcggcaa cgctccacca cctggcgcgc gctgtcatcg ctcgaatcgt caccgatcac
     5281 gatttcaaat tcgccatacg tctgcgcatg acagctgtcg atggcctgtt cgagcaagac
     5341 aggccgattg caggtcggaa tgcagatgga gatcagacgc gcggctgccc cactgccgct
     5401 cgtgaggccg gcctcgttcg tggtctgagt catgggtgct ccgaaaaccg taacggattt
     5461 catttgaccg tgccggaccg cgaatatctg tacggccacc cacacatccg ccatgggcga
     5521 aggcgcaata tcaaagccta caggaggccg gcgtcatcgt ttcaaacgcg ctgctgggtg
     5581 cggcgacgca ccgacagcat tcatgatcga ctccatcatc gaatgactca cccgccggat
     5641 agcgcaccat ggaaagaagt atcgtcaaga acatcttcat caattttgca ggagccatcg
     5701 ctccgacctt cgtctccctc gttaccgtgc ccgcctatat tcacctgatg ggcgtcgagc
     5761 gctacggggt catcaatctg gtgtggacgc tcatcggcta cttcagcgtg ctcgatctcg
     5821 gcacgagtct cgccaccgaa aatcagatct ccaaggcgcg cgccgccaat gacgactcga
     5881 tcgatctcat cttctggagc gcgtggttca tgaatctggg caccggcatc gtgggcggcc
     5941 tgctggtgta cctcggcacg tttctctaca tcacgcacgg cgtgaagatc gagcccgcct
     6001 ttcagcgcga agtcatggcg agcctgccgt ggatcgcggt ggccgtgccg atcgcgaacg
     6061 tctcgtgggt gtttgccggc gccatcaacg gcgtggaacg cttcaccagc ttcaatatca
     6121 accagacgct gggcacggcg ctgttccaat tgctgccgct ggcggcgatc ttctgctttt
     6181 cgccctcgct cgcggtggtg attccggccg cggtggtggc gcggctgcta ggcggcgtgt
     6241 tgctcggcgc ggcggcgttc cgcgcggtcg gcattcgtcg cgtgcggatg ccgcagtggc
     6301 gcgtgatggc cgagctcttt cgctacgggc gctggatgct gatgttctcg ggcgccaaca
     6361 tgattgcgtc gaccctcgac cgggtgctgg tgggcgcgct gctcggcgcg cgcttcgtca
     6421 cctactatgc gaccccgcag aacctcatca cgcgtctgaa cctgctgccg atcgcgatgg
     6481 tgcgcacact gttcccgcgc ctgtcggccg tttcgcgcgc cgacgccgac gcgctcgcgc
     6541 acagctccct cgcttttctg aacggggcct tcacgccctg cgtgatcgtc gcgctgttcg
     6601 cgctcaagcc gtttctgacg ctatggctgg ggccgacggt cgccgccgcg gcccccgtcg
     6661 ccagcgtgct cgtgctgggc gtgtggctga gcggccaagc gggcattctg gggattctga
     6721 ttcaggcgca gacgaatccc gcttatatcg cgggcgtgag ctggcttcaa ttgccggtgt
     6781 tcgccggcgc gctgtggtgc gggattcact ggttcgggat catgggcgcg ggtgtcgtcg
     6841 tcgtgatgaa gtcgctgttc gactatgccg tgctgctgtt tttcgcgcga ctgcatgtcg
     6901 ggaccatcgt gcgcaatatg ctggcccacc tggcgtttct ggtcgttgcg ctggtgctcg
     6961 ccgactcgat caccgcattg ccgagtttga tcgccgcagc ggtggtcatg gcggcggcga
     7021 atctggggat gtcgttgcat gggtccagcg agctcaggag tgtcgtgtat aagctgtgga
     7081 cgcggctgac gttgtcggca agttagttgg gcggtagaag cgggtctgga ttcaggcccg
     7141 ctttacctct tcgcttcacc tctgcgcttc acctcttctc ttcacctctg cgcttcacct
     7201 ctttcttctc cg
//
LOCUS       DATASH010000260         3017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4189363,
            whole genome shotgun sequence.
ACCESSION   DATASH010000260 DATASH010000000
VERSION     DATASH010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4189363"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VHV99_07255"
     CDS             complement(<1..210)
                     /locus_tag="VHV99_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006052849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEX3635272.1"
                     /translation="MTEDVRMERDTFGEIAVPNARLWGAQTQRSLQNFRISSEKQSPE
                     LITALAVIKRAAAEVNQGLGVLDENK"
     gene            complement(227..1816)
                     /locus_tag="VHV99_07260"
     CDS             complement(227..1816)
                     /locus_tag="VHV99_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HEX3635273.1"
                     /translation="MRHFFLACCATAFLTACATKPPATAFDRPVTHALPATDTTPLHT
                     ALAPLEAAHPNESGFRLLSRGTDALQMRIALARAATKTLDMQYYIANEDTTGKLLLGA
                     ALYAADHGVRVRMLVDDLNFKDIDQLMAGLNSHDNIEIRVFNPFGSAQEGMFERTTNV
                     FTQIGHFTRRMHNKSMITDNQLAIVGGRNLGDEYFSASETLQFRDLDVLAAGPVVADV
                     SASFDDYWNSSIAYPLRVLNKQTFDAKVLDQTRDDLRQHWRANADPYNAKPLNATPLA
                     SQIVQDQLGLTWAPAEFKTDLPEKIVHPSPDYVSPPLQRLAELMRDAQKDFLIISPYF
                     VPHESGVKAAGELTHRGVRIAVLTNSLAATDAVAVQAGYSPFRVPLLQQGVELYEFKP
                     QQNSPSAGLTGSRSRASLHAKAYVIDRKILVIGSMNLDPRSANLNTEQTLVIHSPPLA
                     EQVARIFEHAIAPEASYHVTLADPAQLAYLRSIGAPLSPLVWTDVEDGTRRTYIFDPQ
                     AGFYRNALTGLFSLLPVNAEL"
     gene            complement(2313..>3017)
                     /locus_tag="VHV99_07265"
     CDS             complement(2313..>3017)
                     /locus_tag="VHV99_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HEX3635274.1"
                     /translation="LRMPPDLTAGMVLVGSVASGTASNVMIYLARGDVALSVTISALS
                     TLVGVFATPLLTRLYVDASIAVDVHGMLMSILQIVALPIVVGLVVNQLFNKAVRKVEP
                     ILPLISMVAILLIIGAVVGGTQKNIASVGLVVMLGVVLHNGIGLLGGYWGGRLLGFDE
                     AVCRTLAIEVGMQNSGLAATLGKLYFTPIAALPGALFSVWHNLSGSLLAGYWAGRPAK
                     GSTHPDGERGLNAERG"
BASE COUNT          546 a          899 c          983 g          589 t
ORIGIN      
        1 cttgttctcg tcgagcacgc ccagaccctg attgacctcg gcggccgcgc gcttgatgac
       61 ggccagcgcg gtgatcagtt cgggcgattg cttctcggac gaaatacgga aattctgcag
      121 cgagcgctgg gtctgcgcgc cccatagacg agcgttcggt acggcgattt caccgaacgt
      181 gtcgcgctcc attcgtacgt cttcagtcat ggtccaactc cgcttttcaa agttctgcgt
      241 tgacaggtag tagggagaat agacccgtta gcgcgttccg gtaaaaaccc gcttgcggat
      301 cgaaaatata ggtgcggcgt gtgccgtctt cgacatcggt ccacacgagc ggcgagagcg
      361 gcgcgccgat cgagcggaga taggcgagtt gcgccgggtc cgcgagcgtg acgtggtagc
      421 tcgcttcggg tgcgatggcg tgttcgaaga ttcgcgcgac ctgttcggcg agcggcgggc
      481 tatggatgac cagcgtctgc tcggtattca gattggctga gcgcggatcg agattcatcg
      541 agccgatgac cagaatcttg cggtcgatga cataggcttt ggcatgcagg ctggcgcgtg
      601 agcgcgatcc ggtgagaccg gcgctcggcg aattctgctg cggtttgaat tcatacaact
      661 ccacgccctg ttgcagcagc ggcacgcgga aagggctata gccggcttgc acggccaccg
      721 cgtcggttgc ggcgagcgag ttggtcagca cggcgatgcg cacgccccga tgcgtcagtt
      781 cgccggccgc tttgacaccg gattcgtgcg gcacgaagta aggcgaaatg atcagaaaat
      841 ccttctgcgc gtcgcgcatc agctcggcca atcgctgcag tggcgggctg acatagtccg
      901 gcgacggatg cacgattttc tccggcaggt ccgtcttgaa ttcggccggt gcccacgtca
      961 agccgagttg atcctggacg atctgcgagg cgagcggcgt cgcgttcagc ggtttcgcgt
     1021 tgtacggatc ggcgttggcg cgccagtgct ggcgcaaatc gtcgcgcgtc tggtcgagca
     1081 ctttcgcatc gaacgtctgt ttgttcagca cccgcagcgg gtaagcgatg ctgctgttcc
     1141 agtagtcgtc gaaactcgcg gagacgtcgg cgacgaccgg accggcggcc agcacgtcga
     1201 ggtcgcggaa ctgcagcgtt tcgctggcgc tgaaatattc gtcgccgagg ttgcggccgc
     1261 cgacgatcgc caactggttg tcggtgatca tcgacttgtt gtgcatgcgg cgcgtgaaat
     1321 ggccgatctg cgtgaacacg ttggtggtgc gttcgaacat gccttcctgc gcgctgccga
     1381 acggattgaa gacgcggatc tcgatgttgt cgtgtgagtt cagacccgcc atcagctggt
     1441 cgatgtcctt gaagttcaga tcgtcgacca gcatgcgcac acgtacaccg tggtcagccg
     1501 cgtagagcgc ggcgcctagc agcagcttgc cggtggtgtc ctcgttggcg atgtagtact
     1561 gcatgtcgag ggttttcgtc gcggcacgcg cgagcgcgat gcgcatctgc aaggcgtcgg
     1621 tgccgcgcga caggagccgg aatccggact cgttcggatg cgcggcttca agcggcgcga
     1681 gggccgtgtg aagcggtgtt gtgtcagtgg cgggcagtgc gtgggtgacc ggacggtcga
     1741 acgcggtggc gggcggtttg gtggcgcagg cggtcaaaaa tgccgttgcg cagcacgcca
     1801 ggaagaaatg tctcatggtc cgccaatccc tcaacgggac ttccttcggg gcggacggcc
     1861 tctttcgagg cggaggactt ccttcggggc ggaagacttc cttccgggct ggggtttttc
     1921 tccagagcgt gctgaacgcg cactcgcggc gcaatgcggt ctgattcggg ttgtgatctt
     1981 gatgccacga cacgctgttt cccccttgtt tgcacgcttc tcatgacggt ggcgcgtgtg
     2041 gcttgtccac tctgtcttaa aaatgcattc taaggggaat caggccggag agctttcgtt
     2101 cttccatgtg accgaggcga ggcacattag cgagatttgt gagcctgcgt ggcgctttct
     2161 atccgcacat tggaggcgtg cggatagatc ccgctagtca aagcagaagc ttgcgacgca
     2221 cggcgttcga gggccgccgg agtgtcgagc gacgagcgct tgagcatcgc tcagtggccg
     2281 ctagcgtgtc gctcgataac cgcttgacgg tattacccgc gttcggcatt caacccgcgc
     2341 tcgccgtccg gatgcgtgga ccctttcgca gggcgccccg cccaataccc cgcgagcagc
     2401 gagccggaca ggttatgcca caccgaaaac aacgcgccag gcaacgccgc gatcggcgtg
     2461 aaatacagct tgccgagcgt cgcggcgagc ccggagttct gcatgcccac ttcgatggcg
     2521 agggtgcggc agacggcttc gtcgaaaccg agcaaacggc cgccccagta accgccgagc
     2581 aaaccgatgc cgttgtgcag caccacaccc agcatcacca cgagaccgac cgacgcgatg
     2641 ttcttctgcg tgccgcccac caccgcgccg atgatcaaca gaatcgccac cattgaaatc
     2701 agcggcaaaa tcggctcgac cttgcgcacc gccttgttga acaactggtt gacgacgaga
     2761 ccgaccacga tcggcagtgc gacgatctgc aggatgctca tcagcatgcc gtgcacgtcg
     2821 acggcaatcg atgcatccac atacaggcgc gtaagcagcg gcgtcgcgaa cacgccgacc
     2881 agcgtcgaca acgcgctgat cgtcaccgac agcgcgacgt cgccgcgcgc cagatagatc
     2941 atcacgttcg acgccgtgcc gcttgccacg ctgcccacca gcaccatgcc ggcggtcaga
     3001 tccggcggca tgcgcag
//
LOCUS       DATASH010000261         4450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2013313,
            whole genome shotgun sequence.
ACCESSION   DATASH010000261 DATASH010000000
VERSION     DATASH010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4450
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2013313"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /gene="bioD"
                     /locus_tag="VHV99_07270"
     CDS             complement(<1..668)
                     /gene="bioD"
                     /locus_tag="VHV99_07270"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HEX3635275.1"
                     /translation="MSTALSLFVTGTDTEIGKTFVSAALLRGFVREGLQAAAMKPIAA
                     GAFELNGVLHNEDADQLDAASSVLLPPEIRTPYLLKEPAAPHIAAALENVTFDLDHIV
                     ECHAHALKQAEIVVVEGVGGFRVPLTATQDTADLAVALKLPVMLVVGMRLGCISHALL
                     TAEAIAARGLTLAGWVANRIDPDMTFPDENIASIREHLAREYDAPLLGIVPHLTPASP
                     ELAAG"
     gene            complement(665..1849)
                     /gene="bioF"
                     /locus_tag="VHV99_07275"
     CDS             complement(665..1849)
                     /gene="bioF"
                     /locus_tag="VHV99_07275"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEX3635276.1"
                     /translation="MHLLDTLTEGLKDIDARGLRRRRRTADTPCAAHMTVDGREIIGF
                     ASNDYLGLAAHPQLIAAIAEGAQRYGAGSGGSHLLGGHSRAHAQLEDDLAEFAGGFVD
                     NARALYFSTGYMANLATLTALAGRGTTLFSDALNHASLIDGARLSRADVQIYPHCDME
                     ALSAMLDASDANVKVIVSDTVFSMDGDIAPLPRLLELAEQHGAWLIVDDAHGFGVLGP
                     QGRGAVAQAALRSPNLISIGTLGKAAGVSGAFVAAHETVIEWLVQRARPYIFTTASVP
                     AAAHAVSASLRIIGGEEGDARRAHLQQLIERTRAMLKATPWLPVDSHTAVQPLIIGAN
                     DATLDIAATLDRAGLWVPAIRPPTVPTGTSRLRISLSAAHSQADLDRLEAGLQQLGAK
                     AA"
     gene            complement(1849..3198)
                     /gene="bioA"
                     /locus_tag="VHV99_07280"
     CDS             complement(1849..3198)
                     /gene="bioA"
                     /locus_tag="VHV99_07280"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEX3635277.1"
                     /translation="MEKSASPEDWITRSLRAVWHPCTQMKHHEHLPLIAVARGQGAWL
                     YDRTNNRYLDAISSWWVNLFGHANPRINAALKNQLDTLEHAMLAGCTHEPAIELAERL
                     SALTNNTLGHAFFASDGASAVEIALKMSFHAWRNRGFADKQEFVCIANSYHGETIGAL
                     GVTDVALFKDAYDPLIRNAHVVASPDARLAQAGETSADVARRALDQVRALFEARAAKI
                     SALIVEPLVQCAAGMAMHDASYIAGLRALCDQYSVHLIADEIAVGCGRTGTFFACEQA
                     GIWPDFLCLSKGISGGYLPLSIVLSRDEIFEAFYHDDTARGFLHSHSYTGNPLACRAA
                     LATLDLFASDNVLAVNAQKSAKLKSALAPLADHEQVRNLRQCGTIFAFDAVIDDAQQA
                     KTFARRFFENGLQRELLLRPISTTVYLMPPYILDDEEIALLASRTRETFEATLVETR"
BASE COUNT          761 a         1290 c         1495 g          904 t
ORIGIN      
        1 ccggccgcga gttcgggcga agccggagtc aaatgcggca cgatgccgag caacggcgcg
       61 tcgtattcac gcgcgagatg ctcgcggatc gaggcgatgt tttcgtcggg gaacgtcatg
      121 tccggatcga tgcgattcgc cacccagccg gcaagcgtca gcccacgcgc ggcgatcgct
      181 tcggcggtga gtaatgcgtg gctgatgcaa ccgaggcgca tgccgaccac cagcatgacc
      241 ggcagcttca acgcgacggc gagatcggcg gtgtcctgcg tcgcggtcag cggcacacga
      301 aaaccgccga cgccttcgac cacgacgatc tcggcttgct tcaaagcgtg tgcatgacac
      361 tcgacgatgt ggtcgagatc gaacgtgacg ttttccagcg cggcggcgat gtgcggtgcg
      421 gccggttcct tcagcagata cggcgtgcga atctccggcg gcagcaggac gctcgacgcg
      481 gcgtcgagtt gatcggcgtc ttcgttatgc agcacaccgt tcaattcgaa tgcgcccgcg
      541 gcgatcggtt tcatcgcggc agcttgcagt ccttcgcgaa cgaagccgcg caaaagcgcc
      601 gcggagacga aggtcttgcc gatttccgta tcggtgccgg tgacgaatag ggacagcgcc
      661 gtgctcatgc cgctttcgcc ccgagttgtt gcaagcccgc ttcgagccgg tccagatccg
      721 cttgcgagtg cgcagcggag agcgaaatgc gcagccgcga tgtacccgtg ggcaccgtgg
      781 gcggacggat cgccgggacc cacaagccgg cgcgatcgag cgtggccgcg atgtccagcg
      841 tggcgtcgtt tgcgccgatg atcagcggct gcacggcggt gtgcgaatcg accggcaacc
      901 acggcgtcgc tttcaacatc gcgcgcgtgc gttcgatcaa ctgctgaaga tgcgcacgcc
      961 gcgcgtcgcc ttcttcgccg ccgatgatgc gcagactcgc cgacaccgca tgcgccgcgg
     1021 cgggcacgga agcagtggtg aagatgtatg gacgcgcgcg ctgcacgagc cattcgatca
     1081 cggtttcatg cgcggcgacg aacgcgcccg atacacccgc cgccttgccg agcgtgccga
     1141 tcgaaatcag atttggcgaa cgtagcgcgg cttgtgcaac cgcgccacgt ccttgcggac
     1201 cgagcacgcc gaaaccgtgc gcatcgtcga cgatcagcca tgcgccatgc tgttccgcga
     1261 gttcgagcag acgcggcaag ggcgcgatat cgccgtccat gctgaacacg gtatcggaga
     1321 cgatgacttt gacgttcgcg tccgacgcgt cgagcatcgc gctcaacgcc tccatgtcgc
     1381 agtgcggata gatctgcacg tcggcgcgag acaggcgtgc gccatcgatc agcgacgcgt
     1441 ggttcaacgc atcggagaag agcgtcgtgc cgcggcccgc gagggcggtg agcgtggcga
     1501 gattcgccat gtagcccgtg ctgaagtaga gcgcgcgcgc gttgtcgacg aagccgccgg
     1561 cgaattcagc gagatcgtct tccagctgcg catgcgcgcg cgagtggccg ccgagcagat
     1621 gcgaaccgcc gctgcccgcg ccatagcgct gggcgccctc cgcgattgcc gcgatcagtt
     1681 gcggatgtgc cgcgagaccg agatagtcgt tactggcgaa gccgatgatc tcgcggccgt
     1741 cgacggtcat gtgtgcggcg cacggcgtgt cagccgtgcg gcggcgacgg cgcaggccgc
     1801 gcgcgtcgat gtccttgagg ccttcggtga gtgtgtcgag caggtgcatt agcgcgtctc
     1861 cacgagcgtg gcttcgaagg tttcgcgagt gcgcgaggcg agcagcgcga tttcttcgtc
     1921 gtcgaggatg tagggtggca tcagatacac cgtcgtcgaa atcggacgca acagcagctc
     1981 gcgctgcaac ccgttttcga agaagcggcg cgcgaatgtt ttggcttgct gagcgtcgtc
     2041 gatcactgcg tcaaatgcga agatcgttcc gcactgacgc agattacgca cctgctcgtg
     2101 gtcggcgagc ggcgcgagcg cggacttcag cttcgcggac ttctgtgcgt tgacggcgag
     2161 cacgttgtcg cttgcgaaca gatcgagcgt ggcgagcgcc gcgcggcacg cgagcggatt
     2221 gccggtgtac gagtgcgagt gtaggaagcc gcgcgcggtg tcgtcgtgat agaaggcttc
     2281 gaaaatttca tcgcgcgaca acacgatcga gagcggcaga tacccgccgc tgatgccttt
     2341 cgacagacac aggaagtccg gccagatgcc ggcttgttcg caggcgaaga aggtgcccgt
     2401 gcgtccgcag ccgacggcga tttcatcggc gatcagatgc acgctgtact gatcgcagag
     2461 ggcgcgcaat ccggcgatat acgacgcgtc gtgcatcgcc atgccggccg cgcattgcac
     2521 gagcggctcg acgatcagcg cggagatttt cgcggcgcgt gcctcgaaca acgcgcgaac
     2581 ctgatcgagc gcacggcgcg cgacatcggc cgaggtttca ccggcttgcg cgaggcgcgc
     2641 atcgggcgaa gcgacgacgt gcgcgttgcg gatcagcgga tcgtaggcgt ccttgaagag
     2701 ggcgacgtcg gtgacgccga gcgcgccgat ggtttcgccg tgatagctat tggcgatgca
     2761 gacgaattcc tgcttgtccg cgaagccgcg attgcgccac gcgtggaagc tcatcttcag
     2821 tgcgatttcg acggcggacg cgccatcgga tgcgaagaag gcgtggccga gcgtgttgtt
     2881 ggtgagcgcg ctgaggcgct ccgcgagttc gatggcgggc tcatgcgtgc agccggcgag
     2941 catggcgtgt tctagcgtgt cgagctggtt tttcagcgcc gcgttgatgc gcgggttggc
     3001 gtggccgaac aggttgaccc accaggagct gatcgcgtcc agataccggt tgtttgtgcg
     3061 gtcgtagagc cacgcccctt ggcctcgcgc gacggcgatg agcggcaggt gctcgtggtg
     3121 tttcatctgg gtgcaggggt gccagacggc acgcaagctg cgggtgatcc agtcttcagg
     3181 ggatgctgac ttttccaaaa ggtactccga ggtgcttttt ttgccgctgc acggctggct
     3241 tttttggggc cttttcattc gcgatgcgtg gttttctttg cgctgcgcgc ggcctttggg
     3301 aggcttgcgg gggtgagatt agctgtttat ttcgtgctgc gcaccagggg cggcggttcc
     3361 ggttttgcct gtggcgctgg ggtttggtgc tgttcgatgc aggtggtttc agattcggta
     3421 tgaaggtcgc tgatcggcgg agttgacgac ggcaaagtag ttggaccttg tgggcgggaa
     3481 tttgggggag atgcctgcgg cggtgagggc tcgtttggtt ttttgccttc ctggcgcttt
     3541 ttgtctgtac gcgtgcggcg ttggggtttt cttgatttgg gttggttgct cgcgcttgtt
     3601 tctgtttgcc tgcggcggtg gcctttgatt gatctggttg cctgcgcggc gcttgtttgt
     3661 ctgtgctttt atggtgtcgg cctttcgttg atctgggttt ttggcctttc cttgaattga
     3721 tattggtcta ttagcgtcgc ccctgtgcgg ggcgggcacc tacttttctt tgcctgccgc
     3781 aaagaaaagt aggcaaaaga aagcggctca ccccgctagc tcttaagcgg gtcccccgca
     3841 cagtgacggt agtggtgcat ctggaatccg tgcccccgca cattcggcgt gagtgacaca
     3901 gccgtcatac ttccggcggc gctgcgcacg ccgaagcgca tttctcaaaa ccgattgctg
     3961 cgtttcgcat tcgcgctcca ttcgcgttta tcagcttagt cggctcattc cgctcattcg
     4021 gtttattcga tttattcggt tcatttggct tattcggttc atttggcatt tcgccgaggc
     4081 gaagccgatg gcccccactt cgcccgaaca gaaccactgg tttgcatgat cgtcccttcc
     4141 ggcgagcacg cagggcgagg cgggaagtat gacggcctgg tcactcacgc cgaatgcgcg
     4201 atggcacaga ttccagatgc accactaccg tcactgtgcg ggggacccgc ttatgcgctg
     4261 gcggggtgag ccgctttctt ttgcctactt ttctttgcgg caggcaaaga aaagtaggtg
     4321 cccgccccgc acaggggcga cgctaataga ccaatatcaa ttcaaggaaa ggccaacacc
     4381 acaaaagcac agacaaacaa gcgccgcgca ggcaaccaga ccaaggaaag cccaacacca
     4441 caaaagcaca
//
LOCUS       DATASH010000262         4759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6562993,
            whole genome shotgun sequence.
ACCESSION   DATASH010000262 DATASH010000000
VERSION     DATASH010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4759
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6562993"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1623)
                     /locus_tag="VHV99_07285"
     CDS             complement(<1..1623)
                     /locus_tag="VHV99_07285"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX3635278.1"
                     /translation="MTSSNTHSPLDSIAALALGEDAPAAPAASVSPANPANPAPISSV
                     APAAAAIAVQTGPSFESLGLSPEIIVALTASGYQVPTPVQQRAIPAALAGRDLLVSSP
                     TGSGKTAAFMLPAIERFSQLEKAKASAPREPHDPNARPARRTQPVARPTMLVLTPTRE
                     LAMQVTAAATTYGKHLRRLRTVSILGGVAYGQQLMLLAKNPEILVATPGRLLDHLERG
                     RIDLSQLQILVLDEADRMLDMGFIDDIETIVAATPATRQTLLFSATIDGKISSLTGRL
                     LKNPERIEIIQKLEAQNNIAQTVHYVDDRDHKDRLLDHLLRDAGLDQAIVFTATKNDA
                     DQLAIRLSDAGFESAALHGDLPQGARNRTIRALRERKVRVLVATDVAARGIDIPGITH
                     VFNYDLPKFAEDYVHRIGRTGRAGRTGTAISLVHHAEQGALKRIERFVRVPLNVSVIV
                     GFEPRRSAPAGGGGGRPGFGGRGRPGGGAGAGGAGRRFGSGGGAGNGGSGKPGGWGNK
                     PASAGGARDSRDGGSRDGYRSGGGSRDGGSGFG"
     gene            complement(2147..2416)
                     /locus_tag="VHV99_07290"
     CDS             complement(2147..2416)
                     /locus_tag="VHV99_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006763999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA-binding protein"
                     /protein_id="HEX3635279.1"
                     /translation="MNELDAQFALAQADVKELPERPGNLTLLRLYALYKQASVGDVQG
                     DKPGFADIAGKYKYDAWTALAGTPQDTAKQQYIGLVESLKSGASA"
     gene            2622..3506
                     /gene="tsaB"
                     /locus_tag="VHV99_07295"
     CDS             2622..3506
                     /gene="tsaB"
                     /locus_tag="VHV99_07295"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007742220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEX3635280.1"
                     /translation="MTRTVLLALDTSTEFCSVALLIADPTAVAAPTACESAPHYPHYS
                     AGEVHVWFSHELTGPVSSTRLLPAVRELFDTAGLKLQDCTAIAFGAGPGSFTGLRTAT
                     GVAQGLAFGLSVPVVPIGTLLACAEFARLHEPSATRVLAALDARMNEAYWADFRWDPV
                     AGAAGEWATVQAASLDLPEAIVRPDEPFTLAGNAAAAFGERLPMPAAPACRIDFDALP
                     HALPVALAGLRALRAGRTVAPDQAAPEYVRNKVAQTTAERMDARAAKDAAAAAAAGEQ
                     ANQADPAAPVVWTTEPPR"
     gene            3503..3997
                     /gene="rimI"
                     /locus_tag="VHV99_07300"
     CDS             3503..3997
                     /gene="rimI"
                     /locus_tag="VHV99_07300"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018417621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEX3635281.1"
                     /translation="MSGVLMADRYLSSMTEADLDEVAAIEKLAYEFPWTRGNFQDSLR
                     NGYFGVCLRHVTGTLLGYCVLMPVVDEMHLLNLCVAPQAQHAGAGLTLLREAVRISRH
                     QKLEGVLLEVRPSNPRAIQLYERFGFATIGRRKNYYPARHRSREDALVMRLTFSKEAV
                     DGAA"
     gene            3984..>4759
                     /locus_tag="VHV99_07305"
     CDS             3984..>4759
                     /locus_tag="VHV99_07305"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX3635282.1"
                     /translation="MALHKSMLEEFGLGTVWVRRGAASESASAEASRAVHAQSTDSTA
                     NAPDRKAAVEVHRPALPMPIEEARAPAARKADPVDTLTALDAALSAAQASQRDAHQAP
                     PARIDSPRVDAAEPPAFDDLPWLDDVPPQSLAVSETVDDTAADIATLDWETLAARVSV
                     CERCRLCERRTNSVFGVGDREADWMLIGEAPGENEDKLGEPFVGQAGKLLDSMLHAVA
                     LSREDNVFIANVIKCRPPGNRNPELDEVARCEPYLKRQVEL"
BASE COUNT          872 a         1452 c         1555 g          880 t
ORIGIN      
        1 accgaagccc gaaccaccgt cacgcgatcc accgccgctg cgatagccgt cacgcgaacc
       61 accgtcgcgt gaatcgcgag caccgccggc gcttgccggc ttgttgcccc agccgcccgg
      121 cttgcccgaa ccgccgttgc cagcaccgcc gccgctaccg aagcgacgac ctgcgccgcc
      181 tgcaccagcg ccaccgcccg gacggccacg gccgccaaag ccaggacgac cgccgccacc
      241 gccggccgga gccgaacgac gcggttcaaa gccaacgatc acgctcacat tcagcggcac
      301 acgaacgaaa cgctcgatgc gcttcagcgc gccttgctca gcgtgatgca caaggctgat
      361 tgccgtgccc gtacggccag cacggcccgt acggccaata cggtggacgt agtcttccgc
      421 gaacttcggc aggtcgtagt tgaacacgtg cgtgatgccg gggatgtcaa taccgcgagc
      481 ggcgacatcg gttgcgacca gcacgcgcac tttgcgctcg cgcagtgcac gaatcgtgcg
      541 gttacgcgcg ccttgcggca gatcgccgtg cagggcagcc gattcaaaac cagcgtccga
      601 caaacggata gccagttggt cagcgtcgtt cttggttgcc gtgaaaacga tagcctgatc
      661 caggccggca tcgcgcaaca gatggtcgag cagacgatcc ttgtgatcgc ggtcatcgac
      721 gtaatgcacg gtttgcgcaa tgttgttctg cgcttcgagc ttttgaatga tctcgatgcg
      781 ttccgggttc ttcaacaggc ggccggtcag cgagctgatc ttgccgtcga tggtcgccga
      841 gaacagcagc gtctgacgcg ttgcgggcgt agcagcaaca atcgtttcaa tatcgtcgat
      901 gaaacccatg tccagcatgc ggtcggcttc gtcgagcacg aggatttgca gttgcgacag
      961 gtcaatacgg ccgcgttcca gatggtccag cagacggccc ggcgtagcaa ccaggatttc
     1021 cgggttcttc gcgagcagca tcaattgctg gccgtacgca acgccgccga gaatgctgac
     1081 cgtgcgcagc cggcgcagat gcttgccgta cgtggtcgca gcggcagtaa cctgcatcgc
     1141 gagttcgcgg gtcggggtca ggaccagcat ggtcggacga gcgaccggct gggtacggcg
     1201 ggccgggcgg gcgttcgggt catgcggctc acgcggagcc gaggccttgg ccttttcgag
     1261 ctgcgagaaa cgttcgatag cgggcagcat gaatgctgcg gtcttgcccg aaccggtcgg
     1321 gctggaaacc agcaagtcgc ggccggcgag cgctgcggga atcgcgcgct gctgaaccgg
     1381 cgtcggcact tgatagccag aggccgtcag cgcaacaatg atctcaggcg acagcccgag
     1441 cgactcgaag ctcgggccag tctggacagc gatagcagca gcggcgggtg caactgaaga
     1501 aataggggca ggattagcag gattagcagg cgaaacagat gctgccggcg cggcgggtgc
     1561 atcttcgccg agtgccagtg cagcgatgga gtccagcggg ctatgcgtgt tgctcgaagt
     1621 catgtctttc cttgaaacgg ggaataaggc gtgtggcgcc aatcggcata cccaagtcgt
     1681 cagattcagc gagcaaagaa gcagcgagca aatcgaaaaa cgggggcagc tcgcaacaat
     1741 aaaggcgagc ttttcgttag aagctgtatc ggattcgaca ggccgaggcg gctttgtcgt
     1801 cagcctgatg catgatcggc cgagaacggc ctagcaggga ggggacagtt tctaaactgg
     1861 attggagcaa aacgctacga ggcgccgcgc tgcgcggatt gaatcagcta atgaatcgat
     1921 taaaagcatc aattcacccg gtcacagtga agcgcaggca agagtatagc ggcaattgtt
     1981 gcgctgcgcc agattttaag acagtttgat tcatcttaca gtttgccggg ggcgggggag
     2041 atcgcctgca tcctcgccac attcattgcg cctgcatctt gatatagcga tgccaggcgc
     2101 ttaacggccg cccagttgaa gccgtccggc ggtggcccga ctggttttac gccgaggcac
     2161 cgcttttcag cgattccacg agcccgatat attgctgctt cgcggtgtcc tgcggcgtgc
     2221 ccgcgagcgc ggtccatgcg tcgtatttgt atttgccggc gatatcggcg aaacccggct
     2281 tgtcgccttg cacatcgccc acgcttgctt gcttgtagag cgcgtagagg cgcagcagcg
     2341 tcaggttgcc cggtcgttcg ggcaattcct tgacgtccgc ctgggcaagc gcaaattgcg
     2401 cgtcgagttc gttgattccg ctcattcggt tctccggaca tgctggttga ggaccgatga
     2461 tcatagcaag ccgggtgtgc ggggttgtcg agggtttctt ccagaattcc aagacgaacg
     2521 gccagagctg gtacgggcgg gcattgtcca tatggcgctc accgcctttc ggttcacgcg
     2581 gtaccgcggt tcacgttaca tcacgcatta caatagcggc catgacacgc accgttttgc
     2641 tcgccctcga tacctcgacc gaattctgct cagtcgcgct gctgatcgcc gacccgacgg
     2701 ccgttgctgc gccaacggcc tgcgaaagcg ctcctcatta tcctcattac tcagcgggtg
     2761 aggttcacgt ctggttcagt catgaattga cgggaccggt ttccagcacg cgcctgctgc
     2821 ccgcggtccg cgaattattc gataccgccg gcctgaagct tcaggattgc acggcgatcg
     2881 cgttcggcgc cgggccgggc tcatttaccg gtttgcgcac ggcaaccggc gtcgcccaag
     2941 ggctggcgtt cgggctgagc gtgccggtgg tgccgatcgg caccttgctc gcatgcgcgg
     3001 agttcgcgcg cttgcacgaa ccatcggcga cacgcgtgct cgccgcgctc gacgcccgca
     3061 tgaacgaggc gtactgggcg gatttccgct gggatccagt ggcgggagcg gcgggcgagt
     3121 gggccacggt ccaggcggca tcgcttgatc tgcccgaggc aattgtccgg ccggacgagc
     3181 cgttcacgct ggcgggcaat gcggcagcgg ctttcggcga acggttgccc atgccggctg
     3241 ccccggcgtg ccgcatcgat ttcgatgctt tgccgcacgc gttacccgtg gcgctcgcgg
     3301 ggttacgcgc gctgcgagcc ggccggacgg tcgcgccgga tcaggccgcg ccggagtacg
     3361 tacgtaacaa ggtcgcgcag accaccgccg agcgcatgga cgcgcgtgcc gccaaagatg
     3421 ccgccgcggc cgccgctgcg ggagagcaag ccaatcaagc cgatcccgcc gccccggtcg
     3481 tttggacgac ggagcctcca cggtgagcgg cgtgttgatg gcggatcgtt atctgtcgtc
     3541 gatgacggaa gccgatctcg atgaggtcgc cgccatcgag aagctcgctt acgagtttcc
     3601 ctggacccgt ggcaatttcc aggattcgct gcgcaacgga tatttcggcg tgtgcctgcg
     3661 ccatgtcacc gggactttgc tggggtattg cgtgctgatg ccggtcgtcg atgaaatgca
     3721 tttgctgaac ctgtgcgtag cgccccaggc gcaacacgcc ggcgccgggc tcacgttgtt
     3781 gcgcgaagcg gtgcgcatct cgcggcatca gaaactcgag ggcgtgctgc tggaagtacg
     3841 gccgtcgaat ccgcgcgcca tccagcttta cgagcgcttt ggttttgcca ccatcgggcg
     3901 gcgcaaaaac tattaccccg cgcggcaccg gtcgcgcgag gatgcactcg tcatgcgtct
     3961 gacgttttca aaggaggccg tggatggcgc tgcataagtc gatgctggaa gagttcggac
     4021 tgggaacggt ctgggtgagg cggggggcag catccgaatc ggcatcggct gaagcgtcca
     4081 gagccgtgca tgctcagtcg acagattcga cggcgaacgc gcccgatcgt aaagctgcgg
     4141 tcgaggtgca tcggccggct ttgcctatgc caatcgaaga agcccgcgcg ccggcggccc
     4201 ggaaagctga tccagtcgat acgctgacgg cgctcgatgc tgccctctcc gccgcgcagg
     4261 cgagccagcg cgacgcgcat caagcgccgc ctgcaaggat cgattcgccg cgggtcgatg
     4321 ccgccgagcc gccagccttc gatgacctcc cctggctcga cgatgtgccc ccgcaatcac
     4381 tggctgtttc ggaaactgtc gatgacacgg ccgccgatat cgcgaccctc gactgggaga
     4441 cgctggccgc tcgcgtttcc gtgtgcgaac gctgccgctt gtgcgagcgt cgcacgaact
     4501 cggtgttcgg cgtgggtgac cgggaagcgg actggatgct gattggtgaa gcgcccggtg
     4561 agaacgagga caagctgggc gaaccgtttg tcggccaggc gggcaagttg ctcgacagca
     4621 tgctgcacgc ggtggcgtta tcgcgtgagg acaacgtgtt tattgcaaac gtgatcaagt
     4681 gccggccgcc cggtaaccgc aatcctgaac tcgatgaagt cgcgcgctgc gagccctatc
     4741 tgaagcggca ggtcgaact
//
LOCUS       DATASH010000263         2301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6364935,
            whole genome shotgun sequence.
ACCESSION   DATASH010000263 DATASH010000000
VERSION     DATASH010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6364935"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            332..922
                     /locus_tag="VHV99_07310"
     CDS             332..922
                     /locus_tag="VHV99_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635283.1"
                     /translation="MVLNSVFVERIYSRDGHRLPRGFRYAGQGLLDIETYLMQGDPRV
                     ALGKLLDRIRSVGGEPQFAPGFLEHLERRSRRSERQATPLRHVAWTTGAGSADVVTGS
                     ARETHKVDLSRWASPLVMAGEPVSFTREDDGRYRYAVKGATYHLSVDELNARFAVDNV
                     AEGWEVVHEGLAVKFPQQAAALRARVRALGVDRWLN"
     gene            974..>2301
                     /locus_tag="VHV99_07315"
     CDS             974..>2301
                     /locus_tag="VHV99_07315"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX3635284.1"
                     /translation="MIAWEQGCGKSRLAIALILIAKVRHGLIVVESRLIDEMMKEIAM
                     LPLNADDVKVIGCAADLNDLRRFNLISYERLRMPVDKGASKRVTYAHRLRRRIGLLVA
                     DEGERLANPTSDQSRALWQLSARRRYVLTGSPIPNYPRDAFGLIAFSGGDGTAAQPYG
                     YRLGYLEENWINTVQYAMRGVDRFRDDFVVLEWVTWQFAESLQEGAKREVPKIGNLHG
                     YRAMLAPHIKRRLVAEPEVAKYIQIDPPEFEVETVDWDRGHLATYLRAADEFADWYRS
                     SRDDRKACNLITILARIRAVHFAANFPQYGMEGVDRIGGLTSKQRAVISRMREIAAEG
                     KQAIVFAENPGVLDLLARELESHGVQSVAFHGEIPIKRRVSDKDKRFLTGLATGLLAT
                     KASGRAGYNLPNADYILFYDRSWTWRIEYQAMRRALRWNRKGTLKVLYFHL"
BASE COUNT          436 a          648 c          763 g          454 t
ORIGIN      
        1 agttcaccca tcaagggcgg gcacggtctt attttccgac tgctacgctg ccgcgcggct
       61 tgcaaagaca ccgcaaatcg ccgtcctttc ggcacccgac tgttgaaacg actggcattt
      121 cgaaagtcca atgtaatgcg gcccttgaaa agaagcccga aaacagcgtc attcttcgtt
      181 agtagtctgc ccatgggtaa agatctggcc gaggttcgat atagcagcag cctggcggag
      241 ccgcgtcgcg cagacgtgaa gaacaagagc ccgcagttct tcgagagtca cgttatccac
      301 atgcttgtcc ccagaatatg ttgataaccc gatggtgctc aacagtgtct tcgttgagcg
      361 catctactcg cgcgacggcc atcggctgcc acgcggcttt cgttacgccg gtcaggggct
      421 gctcgacatc gagacgtatc tgatgcaggg cgatccgcgc gtcgcgctcg gcaagctgct
      481 cgaccgcatc cgtagcgtcg gtggtgaacc gcagttcgcg ccaggcttcc ttgagcacct
      541 tgagcgtcgt tcaagacgca gcgagcgtca ggcgacaccg ctcaggcatg tcgcgtggac
      601 gaccggcgcc ggctcggctg atgttgtcac cggatcggcg cgggagaccc acaaggtcga
      661 cttgtcgcgc tgggccagtc cgctcgtgat ggcgggtgag ccggtcagct tcacgcgcga
      721 agacgatggc cgataccgct atgccgtgaa gggcgctacc tatcatctgt cggtcgatga
      781 gttgaacgcg cggtttgcgg tcgataacgt cgctgaaggg tgggaagtgg tgcacgaagg
      841 gctcgcggtc aaatttccac agcaggccgc ggcgctccgc gccagagtcc gggcgctggg
      901 cgttgatcgc tggctgaact agcagtttca gaccgaggat cttgtcgaaa cgctgatgaa
      961 gccgtcgggt tgcgtgatcg cgtgggagca gggttgcggc aagtcgcgcc tcgcgattgc
     1021 gctcattctc atcgcgaagg tcaggcatgg actcatcgtc gttgagtcgc gtctgattga
     1081 cgagatgatg aaggagatcg cgatgctgcc gctcaatgcg gatgacgtga aagtcatcgg
     1141 ttgtgcggcg gacctgaatg atctgcgacg gttcaacctg atctcctatg agcgcttgcg
     1201 tatgccggtc gacaaggggg cgtcgaagcg cgtgacgtat gcgcaccgtt tgcggcggcg
     1261 cattggtctg ctggttgccg acgaaggcga gcggctggcc aatccgacga gcgaccagag
     1321 ccgcgcgctc tggcagcttt cggcgcgacg ccgttatgtg ctcaccggct cgccgatccc
     1381 caactatccg cgtgacgctt tcgggctgat cgcgttctct ggtggtgatg gcaccgcagc
     1441 gcagccatac ggttaccggc tgggctatct ggaggaaaac tggatcaaca ccgtccagta
     1501 tgcgatgcgc ggtgtggacc ggtttcggga tgacttcgtg gtgctggaat gggtcacgtg
     1561 gcagttcgcg gagagcctgc aggaaggggc gaagcgtgag gtgccaaaga tcgggaacct
     1621 gcacggctac cgcgcgatgc ttgcgccgca tatcaagcgg cggctggtgg cagagccgga
     1681 agtcgcgaag tacatccaga tcgacccgcc ggaattcgaa gttgagaccg tggactggga
     1741 ccgtggccat ctggccacct acctgcgagc ggccgacgag ttcgcagact ggtatcgctc
     1801 gtcacgggat gaccgtaagg catgcaacct catcaccatc cttgcgcgta ttcgtgcagt
     1861 gcatttcgcc gccaactttc cgcagtacgg gatggaaggc gtcgatcgca ttggcggcct
     1921 gacgagcaaa cagcgcgcgg tgatctcacg catgcgggag attgcggccg agggaaagca
     1981 ggcgatcgtg tttgcggaaa acccgggcgt gcttgatctt ctcgcacggg agctcgaatc
     2041 gcacggtgtg cagtcggtgg cgtttcatgg cgagataccg atcaagcgcc gcgtgtccga
     2101 caaggacaag cggttcctga cagggctggc tacggggctt ctggcgacaa aggcgtcggg
     2161 acgtgcgggc tacaacctgc cgaacgccga ctacattctc ttctacgaca ggtcgtggac
     2221 gtggcgcatc gaatatcagg cgatgcggcg cgcgctgcgc tggaaccgga aggggacctt
     2281 gaaggtgctg tatttccatc t
//
LOCUS       DATASH010000264         5704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3595957,
            whole genome shotgun sequence.
ACCESSION   DATASH010000264 DATASH010000000
VERSION     DATASH010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5704
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3595957"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1695)
                     /locus_tag="VHV99_07320"
     CDS             complement(<1..1695)
                     /locus_tag="VHV99_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX3635285.1"
                     /translation="MPIIKRPHSSNSPAGEDRDAYHRTPGRNAASRDAEAGRRSIGVT
                     IVIWFVGLLATLAVVGALIVGYALVVMGPQLPSLDALTDYRPKVPLRVYTADHVLIGE
                     FGEERRSIVRFQDIPDQMKKAVLAIEDYRFYEHGGVDFVGILRAGVSDITHGGASQGA
                     STITMQVARNFFLSSEKTYTRKIYEMLLAYKIERALTKDQILEVYMNQIYLGERSYGF
                     AAAARVYFGKDLKDITLAEAAMLAGLPKAPSAYNPVVNPKRAKIRQEYILKRMLELKY
                     ISQDQYDQAVKEEIHTKNPGNEYSVHAEYIAEMVRQMMYAQYKDETYTRGLNVTTTID
                     SADQDAAYRAVRKGVMDYERRHGYRGPEGFVELPAPGDDRDQTIDDALTDHPDNGEII
                     AGVVTDANPKQVKAQLVDGTEVTISGDGLRFAAGALSARAAAATKIKPGSIVRLVADD
                     KGNWQITQLPQVEGALVSLTPQDGAIRALVGGFDFNKNKFNHVTQAWRQPGSSFKPFI
                     YSAALDKGIGPATIINDAPLYFPPSTPGGDAWEPKDDDQPEGPMPMRVALEKSKNLV"
     gene            2192..2806
                     /locus_tag="VHV99_07325"
     CDS             2192..2806
                     /locus_tag="VHV99_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018435570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635286.1"
                     /translation="MNKPSERIVVFVTTAQKRAIAATAENLGISVSELMRRAVLSFGA
                     TSEQVKAASIVDRLRAPRAPDALDAALQRVARGTRHARAALPAALQTHKDNASAAPAE
                     SAPAKHADELLGTPAGSSHAPLLPAGVMAALTATAEEREAAAAAEAVARVAAAKAASI
                     ADLAQEPGAMEAQLRNVLKRPRVDAARDEDDPQSDPSTEGGRFA"
     gene            3262..3942
                     /locus_tag="VHV99_07330"
     CDS             3262..3942
                     /locus_tag="VHV99_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004193810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HEX3635287.1"
                     /translation="MSLFDSISRTLKGLLNDAADSVQDPSRDSRQIVRELDESIAKAE
                     NSLIEIQAQVATQQSKRDVAADKAKKYEDGAKRALQSGDEALAREALGAQATAEAERD
                     ALARELVTLEPSVAQLKSQIDDMRGRRNDLNARSNILQAKQQIAQAKDVAATALGGIG
                     GKNLSEDFQKLEDKVALSNARSDARLNSADVKSGKALDDKLADLNRGPSVEDRLEALK
                     KQINTPAQ"
     gene            4088..5269
                     /locus_tag="VHV99_07335"
     CDS             4088..5269
                     /locus_tag="VHV99_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3635288.1"
                     /translation="MKKFLASAFASLLFVSAAAFAVPTVQQIESAMSQGNWQQADAGL
                     SEVLQAHPNNARAHYLYSQVLDREGRYADALAQVQQAKTLDPQIRFTDPSRFAQTEAR
                     IRKDAERAGGNTTSRAANPFVQQNSPAVQQQSAMVPQAPERHGPGVGMWIGIILLIGV
                     IALVLRWTLRRARSQDDSRADDDRRVQLKRATEMLNAVRSLKLDVKLSTVPGHEALEK
                     EVEATEEQLRGLLETLSNSKNPVPPYQLDELEQRIGSMRARADGRPDPYAASAAGTQQ
                     SSYAQEAERFGNPPQAPYPQQGPYQQPPVIVQQGGGGFGGGMGGLLTGVLLGEALNSG
                     RERVVERDVIVDDEARRRGGDGGNGGGLDFGQGSNEWSDNSGGGVDMGSNDDSGGWSD
                     T"
     gene            complement(5390..>5704)
                     /locus_tag="VHV99_07340"
     CDS             complement(5390..>5704)
                     /locus_tag="VHV99_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX3635289.1"
                     /translation="SALAEYHGTSPALREAVRANLHEVSAEMADDVVQLELMPTLVRA
                     TVDEVCTQIVLQLFHLSAEYIDADAARREALVAYAERFIVRLFAGSILLAQHEPARAP
                     PQ"
BASE COUNT         1103 a         1824 c         1787 g          990 t
ORIGIN      
        1 caccaggttc ttcgacttct ccagcgcgac gcgcatgggc atcggcccct ccggctggtc
       61 gtcgtccttg ggctcccacg cgtcgccgcc cggcgtgctc ggcgggaagt acagcggcgc
      121 gtcgttgatg atcgtggccg gtccaatacc cttgtcgagc gccgccgaat agatgaacgg
      181 cttgaagctc gaacccggct ggcgccacgc ctgcgtgacg tggttgaact tgttcttgtt
      241 gaagtcgaag ccgcccacca gcgcgcgaat cgcaccgtcc tgcggtgtga gcgaaaccag
      301 cgcgccttcc acttgcggca gctgcgtgat ctgccagttg cccttgtcgt cggcaacgag
      361 gcgcacgatc gaacccggct tgatcttcgt ggcagccgcc gcgcgcgcgg agagcgcacc
      421 ggctgcaaag cgcaggccgt cgccgctgat cgtcacttcg gtgccgtcga ccagttgcgc
      481 cttcacctgc ttcggattcg cgtcagtcac tacgccggcg atgatttcgc cgttgtcggg
      541 gtgatcggtg agcgcgtcgt cgatggtctg atcgcggtcg tcgcccggtg cgggcagttc
      601 cacgaagccc tccggtccgc gatagccgtg gcgccgctcg taatccatca cgcccttgcg
      661 caccgcgcga taggcggcgt cctggtcggc ggaatcgatc gtggtggtga cgttcaggcc
      721 gcgcgtatag gtttcgtcct tgtactgcgc gtacatcatc tggcgaacca tttcagcgat
      781 gtattcagca tgcacgctgt actcgttgcc cgggttcttg gtgtggatct cttccttcac
      841 tgcctggtcg tactggtcct ggctgatgta tttcagctcc agcatgcgct tcagaatgta
      901 ttcctgacgg atcttcgcgc gcttcggatt gaccaccggg ttgtacgcgg acggcgcctt
      961 cggcaagccg gccagcatcg ctgcttcagc cagcgtgatg tctttcagat ccttaccgaa
     1021 atacacgcgc gcggcggcgg caaaaccgta cgagcgctcg cccagataaa tctggttcat
     1081 gtagacctcg aggatctggt ccttggtcag cgcgcgctcg atcttgtacg ccagcaacat
     1141 ttcgtaaatc ttgcgcgtgt aggtcttctc gctcgagagg aagaagttgc gcgccacctg
     1201 catggtgatt gtgctagcgc cctgcgaggc gccgccgtgc gtgatgtcgg aaacgcccgc
     1261 acgcagaatg ccgacgaagt cgacaccgcc gtgctcgtag aagcgatagt cttcgatcgc
     1321 gagcacggct tttttcatct ggtccggaat gtcctggaag cgcacgatgc tgcgccgctc
     1381 ttcgccgaac tcgccgatca gcacgtgatc cgcggtgtag acgcgcagcg gtactttcgg
     1441 gcgatagtcg gtcagcgcgt cgagcgacgg caattgcggc cccatcacca cgagcgcgta
     1501 accgacgatc agcgccccca ccacggcgag agtcgccagc aagccgacaa accagatgac
     1561 gatggtcacg ccgatcgaac ggcggcccgc ctccgcgtcg cgcgaagcag cgttgcgtcc
     1621 tggagtgcgg tggtaggcgt cccggtcttc accagccgga gaattcgaag aatgcggtcg
     1681 tttgatgatt ggcatgactg gaatagtggg cgcgtaactg aacgcctttc tgcacacgct
     1741 cctggcgcgt gtatggatcg tggtcgagca tcgtggcttg aggcgctcaa caccgcgcgc
     1801 ccctatgccc ccgggcagcc acacggcacg cctgagacat cgtccggcgc aacgtaacag
     1861 cgcattttaa agaaatcaag cgacgtccaa gacagttttt ttgtaagaat tcccttcgcg
     1921 ccgcgtcacg atggtgactc gcggcaggct tacaagcacg cggcgaggct cgccagggtt
     1981 atcaacagaa agtgtggaga ggcctgtgga caaggctgcc ggaatcgcta caactccttg
     2041 atgtcacggg attttcatgc ttcgcccgtt ccgcatcggt gcaactcgtc atatgatgga
     2101 ctcggcgcgc gagtcagcac cgccgcgcgc gacggcgtac gccagataaa acgccttgaa
     2161 cagcccatgc caacaagtat gcgagccgac tatgaacaag cccagcgaac gcatcgtcgt
     2221 attcgtcacg accgcgcaga aacgcgcgat cgccgccacc gcagagaacc tgggcattag
     2281 tgtcagcgaa ctgatgcggc gcgcggtatt gagcttcggc gcgaccagcg aacaggtgaa
     2341 agcggccagc atcgtcgacc gcttgcgtgc gccgcgcgcg ccggacgcgc tcgacgcggc
     2401 gctgcagcgt gtcgcgcgcg gcacgcggca cgcacgggca gcgctcccgg cagccttgca
     2461 aacccacaag gacaacgcat ccgctgcgcc ggcggaaagt gcacccgcaa agcatgccga
     2521 tgaattgttg ggcacgcccg caggttcaag ccatgcaccc ttgctgccgg ccggcgtgat
     2581 ggccgccctc accgctaccg ccgaggaacg ggaggcggcc gccgcggccg aagccgtagc
     2641 gcgcgttgcg gcggccaagg cggcgtccat cgcggacctg gcgcaggaac cgggtgccat
     2701 ggaagcgcag ttgcgcaacg tgctcaaacg cccgcgcgtc gacgccgcac gggatgaaga
     2761 cgatccgcaa agcgatccga gcacggaagg cggccgcttt gcgtgaacgg gaagcgatgg
     2821 gggccaacat ctccatcgat tcacgcgatc gagtgctcag tgtcgcaaac gcgggggatc
     2881 gcgagatatc gctcacacag gagcagcaac actgctaacg ctcactcacg cctcccgccc
     2941 gccacgctca ttcccccacc acacccgccg cgcacatggt ttggcgttgc gccatcgcgc
     3001 cctttaagtc ggttttaaga gaaggcgtgg tgtaatatcc ccgagcagct aaatgactgg
     3061 ccgcgcgtct taaacgccgg gattttttgc aatccggttt caaaacctga attttcggcg
     3121 ccgctattgc aatcggcgcg ggcgccccga actccggtcc gaacgttgcg cagcttctcg
     3181 cgcagcggcg taaagtagca tttgaaagca gttaaagcgc gtgggaatta ccagcgccga
     3241 tttcattctt tggaggtttt catgtcgctt tttgacagca tttcgcgcac gcttaaaggt
     3301 ctcctgaacg acgcagccga ttccgtgcaa gatccgtcgc gcgattcgcg ccagatcgtg
     3361 cgcgagctcg acgagagcat cgccaaagcc gagaactcgc tgatcgagat tcaggcgcaa
     3421 gtcgccacgc aacaaagcaa gcgcgatgtc gccgccgaca aggcgaagaa gtacgaagat
     3481 ggcgcgaagc gcgcgctgca atccggcgac gaagcgctcg cgcgcgaagc cctcggcgcg
     3541 caagccacgg ccgaagccga gcgcgatgcg ctggcgagag aactggtcac gctggagccg
     3601 tcggtggcgc agttgaaaag ccagatcgac gacatgcgtg gccgtcgtaa cgacctgaac
     3661 gcgcgctcga acattctgca ggccaagcag cagatcgctc aggccaaaga cgttgcagcc
     3721 accgcattgg gcggcatcgg cggcaagaat ctgtcggaag atttccagaa gctggaagac
     3781 aaggtggcgt tgtcgaatgc ccgttccgac gcgcgtctga attcggccga cgtgaagagc
     3841 ggcaaggcgc tcgacgacaa gctcgcggat ctgaaccgcg gcccgtctgt cgaagatcgt
     3901 ctcgaagcgt tgaagaagca gatcaacacg ccggcccagt aattgcgtcg gccgtgaacc
     3961 ggctgccatc atgatgtgcg caaccggaac gccggccgca ggaatgccgg ccaccggaat
     4021 gccggccgcc ggatcgccgg ccgccggaat cgcagaagga gacgggcgca gcgcgcccgg
     4081 tccgtaaatg aaaaaatttc tcgcaagcgc attcgcctca ctgctgttcg tgtcggctgc
     4141 ggcgttcgct gtaccgacag tccagcagat cgaatccgcc atgtcgcaag gcaactggca
     4201 gcaggccgac gccggcttga gcgaggtgct gcaggcgcat ccgaacaacg cacgcgcgca
     4261 ctacctgtac agccaggtgc tcgatcgcga aggccgttat gccgacgccc tcgcccaggt
     4321 gcaacaggcc aagacgctcg acccgcaaat ccgcttcacc gacccgagcc ggttcgcgca
     4381 aaccgaagca cgtatccgta aagacgctga acgtgccggc ggcaacacca cgagccgcgc
     4441 ggccaatcca ttcgtgcagc agaattcgcc ggcggtgcag cagcagtcgg ctatggtgcc
     4501 gcaagcgcca gagcggcatg gtcccggtgt cggcatgtgg atcggcatca ttctcctgat
     4561 cggcgtgatc gcgctcgtgc tgcgctggac cctgcgccgc gcccgctcgc aagacgactc
     4621 gcgcgccgac gacgatcgtc gcgtgcaact caagcgcgcg accgagatgc tcaacgccgt
     4681 gcgctccttg aagctcgacg tgaagctctc caccgtacca ggtcatgagg cgctggagaa
     4741 ggaagtcgaa gcgaccgagg aacagttgcg cggtctcctc gaaacgctct cgaacagcaa
     4801 gaatccggtg ccgccgtatc aactcgacga actcgagcaa cgtatcggca gcatgcgcgc
     4861 ccgtgccgat gggcggcccg atccgtatgc cgcgtcggcc gccggcacgc agcagtcgtc
     4921 gtatgcgcag gaagccgaac gtttcggcaa cccgccgcaa gcgccgtatc cacagcaagg
     4981 accgtatcag cagccgccgg ttatcgtgca acaaggcggc ggtggtttcg gcggcggcat
     5041 gggtggtctg ctgaccggcg tgctgctcgg cgaggcgctg aattccgggc gtgagcgggt
     5101 tgtcgagcgc gatgtgattg ttgatgacga agcgcgtcgc cgaggcggtg acggcggcaa
     5161 tggcggcggc ctcgacttcg gccagggctc gaacgagtgg agcgacaaca gcggcggtgg
     5221 cgtcgacatg ggcagcaacg acgattccgg cggctggagc gatacttgac acgatacctg
     5281 cacgcagcac tgcttgaaac gcagccaatc aaacgctaat cgatcaagct gtgataaaaa
     5341 caggctcctg catggggcct gtttttttat gtccatacct tgtatgcggt cactggggcg
     5401 gcgccctcgc cggctcatgc tgcgccagca ggatcgaacc cgcgaacagc cgcacgatga
     5461 aacgctccgc atacgcgacc agcgcctccc ggcgcgcggc atccgcgtcg atatactccg
     5521 ccgacagatg aaacagctgc agcacgatct gcgtgcagac ttcatcgacg gttgcgcgga
     5581 cgagcgtcgg catcaattcc agttgcacga cgtcgtccgc catttccgcc gacacctcgt
     5641 ggaggttcgc ccgcaccgcc tcgcgcagcg ccggcgaggt gccgtgatac tcggccagcg
     5701 cgct
//
LOCUS       DATASH010000265         1883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3992111,
            whole genome shotgun sequence.
ACCESSION   DATASH010000265 DATASH010000000
VERSION     DATASH010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3992111"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..1664)
                     /locus_tag="VHV99_07345"
     CDS             complement(114..1664)
                     /locus_tag="VHV99_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007584601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX3635290.1"
                     /translation="MKAGKAAISLQVRIGLTMGFLAVLMVAIGVLGLLGTSRANRANQ
                     DTYENKLTAATNIGNAEIYIARTRLVLDHVALHPDDPKTAGLIERANGYFNESEQWWQ
                     KFVAQPHEADEASLIDDASERRKAMRGGLSAFINAIQANDHVLVDKITMTQLGDLYNE
                     MNIANQKVKLTLYTNAKRNYEAAESSFHTFITLSILMIVVGVVAAGLSWVALRRAIMS
                     PLNQALEHFDAIAEGDLNRRVEIAKEDEMGTLLRGVEKMQTSLARTVRAVRGSSESIA
                     SATAQIASGMMDLSSRTEEQAASLEETAASMSELTATVKQNADSARTASSLADSASLV
                     ATRGSEVVERVVGTMGSIDASSRKIADITGIIEGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVASEVRSLAQRSSSAAKEIKYLIAESVRTVTVGTELVADAGKTMQEVLHAVRQV
                     TDIVNEIAAAAEEQRSGIEQVDTAVSQMDSITQQNAALVEQATAAAQALNEQSRNLHG
                     FVDVFKLA"
BASE COUNT          353 a          584 c          531 g          415 t
ORIGIN      
        1 gcatgagcgg atgccgcccg ctgtcgggcg atgagcagcg agcgctagtt cagccactgg
       61 tcaccggggc tggttgcacc tggtatttga attcgctggc ggaacgaaaa aaatcaggca
      121 agcttgaaca catcgacaaa accgtgcagg ttacgggatt gctcgttgag cgcctgagcg
      181 gcggcagtcg cctgctcgac gagcgcggcg ttttgctgcg tgatggaatc catctggctg
      241 acggcggtat caacctgttc gatgccggaa cgctgctctt ccgcagcggc tgcgatctca
      301 ttgacaatat cggtgacctg acgcacggca tgcaggactt cctgcatcgt cttgcccgcg
      361 tccgccacga gttcggtgcc gaccgtcacg gtccgtaccg attcggcgat cagatacttg
      421 atctccttgg ccgccgacga agagcgttgc gcgagactac gcacttccga ggcgaccacc
      481 gcgaaaccgc gtccctgctc gcccgcgcgc gcggcttcaa cggccgcgtt cagcgcaaga
      541 atattggtct gaaaggcgat gccttcgata atccccgtga tgtcggcgat cttgcgggag
      601 ctcgcgtcga tgctgcccat ggtgccgacc acgcgctcga ccacttcgct accgcgtgtg
      661 gcgaccagcg aggcgctatc ggcgagcgaa ctcgccgtgc gcgcgctgtc tgcgttctgc
      721 ttcaccgtcg ccgtcaattc gctcatgctg gctgcggttt cctcgagcga tgccgcctgc
      781 tcttcggtgc gtgaagacaa gtccatcatg cccgaagcga tttgcgccgt tgcactcgca
      841 atcgattcgc tactgccgcg cacggctcgc acggtcctgg cgagactcgt ctgcatcttc
      901 tccacgccgc gcagcagcgt gcccatttca tcttctttgg cgatttccac gcgacggttc
      961 aaatcgcctt ctgcgatcgc atcgaaatgt tcgagcgcct ggttcagcgg actcatgatc
     1021 gcgcggcgca gtgcgaccca actcagaccg gcggccacga cgccgaccac gatcatcaga
     1081 atcgacagcg taatgaacgt gtgaaagctc gactccgccg cctcgtaatt cctctttgcg
     1141 ttggtgtaca gcgtcagctt gaccttctgg ttcgcaatgt tcatttcgtt gtagagatcg
     1201 cccaattgcg tcatcgtgat cttatcgacg aggacgtggt cattcgcctg gatcgcattg
     1261 atgaatgcgg aaagaccgcc tcgcatggcc ttgcggcgct cgctcgcatc atcgatcagg
     1321 cttgcctcgt cggcttcgtg cggctgcgcg acgaacttct gccaccactg ctccgactcg
     1381 ttgaaatagc cgttggcacg ttcgatcagt ccggcagtct tcggatcgtc cggatgcaag
     1441 gcgacgtggt ccagcacgag acgcgtacgc gcaatataaa tttccgcgtt gccgatgttc
     1501 gtcgcggcgg tcagcttgtt ctcgtaagta tcctggttcg cgcgattggc ccggctggtg
     1561 ccgagcaaac ccagaacacc gatcgccacc atcaagacag caagaaaccc catcgtcaag
     1621 ccaatacgca cctgcagcga aatcgctgct ttccccgctt tcatttttct tccccaaaga
     1681 cgccgctgtg tagatcacag ccgttcattc ttgcttacgg caagtactta gataggttta
     1741 gagacgattg aaatcgtctg cataatttcc cgagtgctaa cttgttgaaa agagattggc
     1801 cacccggtcg cgaactgaag tgagacgttg caggcaacgc accgggctga aacgacgctt
     1861 caccttgtca cgggcggctc tgg
//
LOCUS       DATASH010000266         4377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2212778,
            whole genome shotgun sequence.
ACCESSION   DATASH010000266 DATASH010000000
VERSION     DATASH010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2212778"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="VHV99_07350"
     CDS             <1..706
                     /locus_tag="VHV99_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEX3635291.1"
                     /translation="LVIRMLSGRHLADASVDAIAASYPNTGYIGFPLGLIAFGSASLT
                     PTTIATILVACVLFAVAIVLIEIGLQTERTPHKLALKVLGSLARNPLIVSPIAGVLFA
                     STHVALPQSAQTFLKLLSGAASPCALVSLGLFLAEKRPSEAGSRGISLLLTGVKLLVQ
                     PALTWWLAARVFGLSPSLVEMAVALAALPTGTGPFMLAEFYRREAHITSRTILLSTVG
                     SVVTLSVLLMLMGHRT"
     gene            800..1693
                     /locus_tag="VHV99_07355"
     CDS             800..1693
                     /locus_tag="VHV99_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX3635292.1"
                     /translation="MPKAHPPELHDDADARSLARHYPRGLRIDPHSHTWAQVLYAVSG
                     VMWVEVGREALVVPPQRAVWLPAGTVHSIHMMSEVEMRNLYLHERNVGHLSRRGDVFE
                     INGLLRELIASIVEHERTHDRDDTYLDAAYRLAMLELGHAPRSSLRIALPDASDRRLD
                     ALCRAVIDNPSIAISFEQHAASVGASVRTLARLFTRELGVGFAEWRRQVQLAVAVSGL
                     AEGRSVSSIARSLGFQPSSFSDMFRRELGAPPTGFDPGGTLSEAASEASPEPAQDKLS
                     ADGGAVRADSRATRSQLNSSR"
     gene            1870..2835
                     /locus_tag="VHV99_07360"
     CDS             1870..2835
                     /locus_tag="VHV99_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEX3635293.1"
                     /translation="MKAIQFKTFGNPETLDYVDLPTPQADAQTAVVQVKAASVNPSDV
                     KNVSGHFEHTVLPRTPGRDFSGVVVDGPQAWLGAEVWGTGGDIGFTRDGTHAEFIKIP
                     LAGLSRKPASLSHAEASAIGVNFVVAWLGTVEYAQLQGGETIAVIGAGGGVGGAVVQI
                     AKARGARVIAIDRHPLSADTPAGRLLDEYIPFDENVTERLKALTGGAGADVVYDSVGG
                     VAFETALSLVKHRGRVLEISATGKRRVEFDLIDFYHNETQLLGVDSAKLGVSESAPLM
                     TALVEGFESGKLQGPAIAQAVPLERAREAYEAVATGTRGRVVITM"
     gene            2890..3066
                     /locus_tag="VHV99_07365"
     CDS             2890..3066
                     /locus_tag="VHV99_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HEX3635294.1"
                     /translation="MKAYLISLVAGVVVGLIYSVMQVKSPAPPTVALVGLLGMLAGEH
                     AIPFVRTWLAAGIH"
     gene            3246..3824
                     /locus_tag="VHV99_07370"
     CDS             3246..3824
                     /locus_tag="VHV99_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HEX3635295.1"
                     /translation="MSEEYEVHGPHDHAVEHAGHHDADPFASRMAVMTAILATIGALC
                     AYQSGNSENLALYYKNEAAIKKTEASNQWNYYQAKGEKENLAELGAALSLGNADAHAK
                     FVADVDKYKQQKEPIRANAEAIEKEVVDNDARSETLLHGHHRWAQATTLIQVAIALSA
                     ITLLTRKNWLRNLSFGVAAAGVITAAVAFFSA"
     gene            3838..4005
                     /locus_tag="VHV99_07375"
                     /pseudo
     CDS             3838..4005
                     /locus_tag="VHV99_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018438306.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
     gene            4198..>4377
                     /locus_tag="VHV99_07380"
     CDS             4198..>4377
                     /locus_tag="VHV99_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3635296.1"
                     /translation="MKPSPDAADDLVANLDESILLPQLGCPAGEIGVAVGDMLERTNL
                     PVIDAAFDLLDLHANE"
BASE COUNT          760 a         1419 c         1422 g          776 t
ORIGIN      
        1 tctcgtcatc cgcatgctga gtggccggca tctggccgat gcgagcgtcg acgcgatcgc
       61 ggcgtcgtat ccgaacaccg gctatatcgg ctttccactc ggcctgatcg cgttcggctc
      121 agccagcctg acgccgacca caatcgccac gatcctcgtc gcgtgcgtgc tgtttgcggt
      181 tgcgatcgtg ctgatcgaaa tcggcttgca gaccgagcgc acgccgcaca agctggcgct
      241 gaaagtgctg ggctctctgg cgcgcaatcc gctgatcgtg tcgccgatcg cgggcgtgct
      301 gttcgccagc acccacgtcg cgttgccgca gagcgcgcag acgtttctga agctgctgag
      361 cggcgccgcc agtccgtgcg cgttggtgag ccttggcttg ttcctcgcgg aaaagcgtcc
      421 gtcggaggcc ggctcgcgcg gcatctcatt gttgctgacc ggggtcaagc tgctggtgca
      481 gccggcgctg acgtggtggc tcgcggcgcg cgtgttcggg ctctcgccct cgctcgtcga
      541 gatggccgtc gcgctggccg cactgcccac cggcaccggc ccgttcatgc tggccgagtt
      601 ctaccggcgc gaggcgcaca tcacctcgcg gacgattttg ctgtcgacgg tggggtcggt
      661 ggtgaccctt tcggtgctgc tgatgctgat ggggcaccgc acgtaacgga atccaggcgc
      721 cgccgtgggg ctcgcgctat gctttgcgcg cggaccaggg ggtccgcgac cttcatacgc
      781 gacagcaaac cgaaccgccg tgcccaaagc ccacccaccc gaactccatg acgacgccga
      841 tgcccgctcg ctcgcgcggc actatccgcg tggactgcgc atcgatccgc attcgcacac
      901 gtgggcgcag gtgctgtacg cggtctcggg cgtgatgtgg gtggaagtcg gccgcgaagc
      961 gctggtcgtg ccgccgcagc gcgcggtatg gctgccggcc ggcactgttc actccatcca
     1021 catgatgagc gaggtggaga tgcgcaacct ctatctgcac gagcgcaatg tgggtcatct
     1081 gagccggcgc ggcgacgtgt tcgagatcaa cggcctgctg cgcgagctca tcgcctcgat
     1141 cgtggaacac gagcgcactc atgatcgtga cgacacctat ctcgacgccg cgtatcgtct
     1201 cgccatgctg gaactggggc acgcgccgcg ctcgtcgctg cgcatcgcgc tgcccgacgc
     1261 ctcggaccgg cggctcgatg cgttatgccg cgcggtgatc gacaacccgt cgatcgcgat
     1321 cagcttcgaa cagcatgcgg cttcggtcgg cgcgagcgtg cgcacgctgg cgcgcctctt
     1381 cacgcgtgag ttgggagtgg gctttgccga gtggcggcgt caagtgcaac tggcggtcgc
     1441 cgtgtcaggc ctggccgagg gacgctcggt cagcagcatc gcgcgttcgc tgggcttcca
     1501 gccgagcagc tttagcgaca tgttccggcg tgagttaggt gcgccgccca ccggtttcga
     1561 tccgggcggc acgctgagcg aggcggcttc tgaggcttcg ccggagccgg cgcaagacaa
     1621 gctgtccgct gacggtggag ccgttcgcgc tgacagccgg gccactcgct cgcagctgaa
     1681 cagttcacgt tgaaagccga gccatttcgc gctgtcaatc aaacaatccc cagcgcggcg
     1741 gacggatcgg cgcgcagcac gtcttgtccg aaattcgaaa gcccttgtct ccacgccttt
     1801 gtaccggtca cctagactac gctcatcggc tgcacgacgc agcacacgaa ccgacaggag
     1861 tgacacgcaa tgaaagcgat tcagttcaag acttttggca accccgagac gctggactat
     1921 gtcgatctgc caacgcctca ggccgacgct cagaccgccg tcgtccaggt caaggcggcc
     1981 tcggtgaatc caagcgacgt caagaacgtg tccggccatt tcgaacacac cgtcctgcca
     2041 cgcacacctg gccgcgactt cagcggtgtg gtggtggacg gcccgcaggc atggctcggc
     2101 gccgaggtgt ggggcaccgg cggcgacatc ggcttcaccc gcgacggcac gcacgccgag
     2161 ttcatcaaga ttccgctcgc cggtctgtca cgcaagcccg cctcgctcag ccatgcggag
     2221 gcctccgcga tcggcgtgaa tttcgtggtc gcctggctcg gcacggtcga gtacgcccag
     2281 ttgcaaggcg gcgagacgat tgccgtgatc ggggcgggcg gtggcgtcgg cggcgcggtc
     2341 gtgcagatcg cgaaagcacg gggcgctcgc gtgatcgcga tcgatcgtca tccgctttcc
     2401 gccgatacgc ccgcaggccg actgctcgac gaatacattc cgttcgacga aaacgtcacc
     2461 gagcgcctga aggcactgac cggcggcgcc ggcgcggacg tggtgtacga cagcgtcggc
     2521 ggcgtggcgt tcgagaccgc attgagtctc gtcaagcatc gcggccgggt gctggaaatc
     2581 agcgccaccg gcaaacgccg cgtggaattc gacctgatcg atttctatca caacgaaacg
     2641 caactgctcg gcgtggacag cgccaagctg ggcgtctccg aatcggcgcc cttgatgacg
     2701 gcgctggtgg aaggcttcga aagcggcaaa ctgcaagggc cggccatcgc gcaggcagtc
     2761 ccgctcgaac gtgcccgcga ggcctacgag gcggtcgcca ccggcacgcg cggccgggtc
     2821 gtcatcacga tgtagttgcc gatgcgacgg cgggcgtggc ggcccgcatt tcagacgggt
     2881 acaacgaaca tgaaagcgta tttgatctcg ctcgtggccg gcgtcgtggt cggtttgatc
     2941 tacagcgtga tgcaggtgaa atcgcccgcg ccaccgacag tcgcactggt cggcctgctc
     3001 ggcatgctgg ccggcgagca cgcgatcccc ttcgtacgga catggctggc cgccggcatt
     3061 cattgacgcg ccattgacgt cctcattgat ctccctcgcg acgatacctt ttccatcacc
     3121 gggcacctca cgctaagatt gaggccgccg ggccggcgcg ccgcgcttgc ggcgtcgccc
     3181 gcgcatgtgc caccgccatg cgcgccgccc gggaaccaca cgactagcct ggctcggaga
     3241 attggatgtc tgaagaatat gaagtgcatg gtccgcacga ccatgcggtt gaacacgcgg
     3301 gccaccacga cgcggacccg tttgcgagcc gcatggccgt gatgacggcg atcctcgcga
     3361 cgatcggcgc attgtgcgca taccagagcg gcaacagcga aaatctggcg ctgtattaca
     3421 agaacgaagc cgccatcaaa aagaccgaag cgtcgaatca atggaactac taccaggcga
     3481 agggcgagaa agagaatctc gctgaactcg gcgcggcgct gtcgctcggc aatgccgacg
     3541 cgcacgcgaa gttcgtcgcc gacgtcgaca aatacaagca gcagaaagag ccgatccgcg
     3601 cgaacgctga agcgatcgaa aaggaagtcg tggataacga cgccaggagt gaaacgctgc
     3661 tgcacggcca tcatcgctgg gcgcaggcca cgaccttgat tcaggtggcg atcgctctga
     3721 gcgcgatcac gttgctgact cgcaagaact ggctgcgcaa tctgtcgttc ggtgtggcgg
     3781 ctgcgggtgt gatcactgcg gccgtggcat tcttttcggc ttgattgtgc gagcggcgcg
     3841 atgctcggca tggtctgcgt ggtggccagc agctggtatg gctttgtcgc gctcacgttg
     3901 tcgcatgcgc ggattgcatc ggcgtatcgg cgctacaaag cgtggatcga tcgcgtgtgc
     3961 ggcgggctga tcgtcgcgtt gggcgtgcgc cagctgatcc gataggccgc ttgcctcgcg
     4021 agcacctcaa cctcaagtca gccctagaac cctccgcgcg ccattttgtc ctgagtcaaa
     4081 gacgcgcgga tcggatgctc agtagtccaa tttcatgaca taaataaagg gcgccgcgat
     4141 cctggatcgc gtgtccgcgc cgcagtacca ggcgcaaccc gtcatggagg actcaccgtg
     4201 aaaccatccc ctgatgctgc cgacgacctc gtcgccaacc tcgacgaaag tattctgctt
     4261 ccgcaactcg gctgcccggc aggcgagatc ggcgtggcag tcggcgacat gctggagcgc
     4321 accaacctgc ccgtcatcga cgcagccttc gatctgctcg acctgcacgc caacgag
//
LOCUS       DATASH010000267         2341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_629038,
            whole genome shotgun sequence.
ACCESSION   DATASH010000267 DATASH010000000
VERSION     DATASH010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629038"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..1756)
                     /locus_tag="VHV99_07385"
     CDS             complement(8..1756)
                     /locus_tag="VHV99_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011356171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635297.1"
                     /translation="MKNTHAFSCLIASRPGFAVALLLSTVVFPPTVLADDDGGLQPNR
                     LLVSRSVYDVNPAIVPGVTPLPFNCVAPNCVTATANGSYPTVFNNNLVDGSFGVTTKI
                     FLDQLTLGGELVGTLEVPNSAQHNVGPNSDQMVTSFSSKSELALNLSTDGQLVSFMGY
                     LAPLGALDVSNSNTPFVFDSTNPVPGRTYRVVAEVDARGHFRFTKTNAYSGNNGRAAI
                     INNTSNGVSFAYLAGNAGNGGNPQPNGIIVGGGAQILTPQDKPLSQQVDPGLPTPVGS
                     FNILQLPGITKTDKIGKDTNFRGLTIFNNVIFFTKGSGGNGVNTLYFVDTSGFDSQSR
                     PLACPAGTGLPSPLATLPTAPISYNAANLQSQGVFPYNMCILNGFPTNLAKSTTAFPF
                     GIWFANATTVYVSDEGNGSNTFSAASGTYTNAAAQTTAGLQKWVFEATTGAWKLAYVL
                     AAGLDLGVPYTISDYPAGNNPATGLPWSPATDGLRNITGRMHRDGTVTIWAVTSTVSG
                     NGDQGADPNRLVRITDTLAARTLPAGESFRTVRTARSGEVLRGVSFTPGTGTPPRDDD
                     DGDDRDHDDDHGHDGH"
     gene            complement(2121..>2341)
                     /locus_tag="VHV99_07390"
     CDS             complement(2121..>2341)
                     /locus_tag="VHV99_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635298.1"
                     /translation="SRANSTLETSHGNNVPGVIIPEAGVRPNGAAKPLQRLACSPEQC
                     HDSCDQFFPAGPRREDCFRACLRKQRDC"
BASE COUNT          380 a          738 c          792 g          431 t
ORIGIN      
        1 cggcgcctca gtgcccgtcg tgaccgtgat cgtcgtcgtg gtcgcggtca tccccgtcgt
       61 cgtcatcgcg cggcggcgtg ccggtgcccg gcgtgaacga cacgccgcgc aacacctcgc
      121 ccgagcgcgc ggtgcgcacc gtgcggaagc tttcgcccgc cggcagtgtc ctggcggcga
      181 gcgtatcggt gatccgcacc agcctgttcg gatcggcgcc ctggtcgcca ttgccgctca
      241 ccgtcgaggt gaccgcccag atcgtgaccg tgccgtcgcg atgcatgcgc cccgtgatgt
      301 tgcgcagacc gtcggtcgcc ggcgaccacg gcaggccggt cgccgggttg ttgccggccg
      361 gatagtcgct gatcgtgtac ggcacgccga gatcgaggcc ggcggcgagc acgtaggcga
      421 gcttccaggc gccggtggtc gcctcgaaca cccacttctg cagcccggcc gtggtctgcg
      481 ccgccgcgtt cgtgtaggtg ccgctcgccg cgctgaaggt gttgctgccg ttgccttcgt
      541 ccgagacgta gaccgtggtc gcattggcga accatatgcc gaacgggaac gcggtcgtgc
      601 tcttcgcaag attggtcggg aagccgttga ggatgcacat gttgtagggg aacacgccct
      661 ggctctgcag attcgcggcg ttgtatgaga tcggcgcggt cggcagtgtg gcgagtgggc
      721 tcgggagccc ggtgccggcc gggcacgcga gtggacgcga ctgcgagtcg aagccggagg
      781 tgtcgacgaa gtacagcgtg ttgacgccgt tgccgccgct gcccttggtg aaaaagatga
      841 cgttgttgaa gatcgtgagg ccgcggaagt tggtgtcctt gccgatcttg tccgtcttcg
      901 tgatgcccgg cagctgcagg atgttgaagc taccgaccgg cgtcggcagg cccggatcga
      961 cctgctggct caacggcttg tcctgcggcg tgaggatctg cgccccgccg ccgacgatga
     1021 tgccgttcgg ttgcggattg ccaccgttgc ccgcattgcc ggcaaggtag gcgaagctca
     1081 cgccattgct cgtattgttg atgatcgcag cgcggccgtt gttgccgctg taggcgttgg
     1141 tcttggtgaa gcggaaatgt ccgcgcgcgt ccacctcggc gaccacgcga taggtccgcc
     1201 cgggcaccgg gttggtcgaa tcgaacacga acggcgtgtt cgagttggag acgtcgagcg
     1261 cgccgagcgg cgccaggtag cccatgaagc tgacgagctg cccgtcggtc gacaggttga
     1321 gcgcgagctc cgacttcgac gagaagctcg tgaccatctg gtccgaattc ggcccgacat
     1381 tgtgctgcgc gctgttgggc acctcgagtg tgccgacgag ctcgccgccg agcgtcagct
     1441 ggtcgaggaa aatcttcgtg gtgacgccga agctcccgtc gacgaggttg ttgttgaaca
     1501 ccgtcggata gctgccattg gcggtcgcgg tcacgcagtt cggcgcgacg cagttgaacg
     1561 gcagcggagt gacgcccggc acgatggccg gattgacgtc gtagacggac cggctgacca
     1621 gcaggcgatt gggctgcaat ccgccgtcat catcggccag caccgtcggc gggaaaacaa
     1681 cggtcgagag cagcagcgcg accgcgaacc ccggccgtga agcaatcagg caagaaaatg
     1741 catgcgtatt cttcatagag gacctcccgt gcgcgcgcgt tgtcgtgcaa cgtcgcgccc
     1801 gggatcatgc ggatatcatg ttacaggttg acgaaggtgc gcgcgccgcg ccgtagaacg
     1861 acagcggcct gttaagtcgg ttgtgaatgg ccccaagcac aagcgcgctt cgccttggac
     1921 cgcaaaaaaa tacctattcg tttataccta tgcgtttctc cccatcaaaa ccgaccgcgc
     1981 acccgctata agacgatccc gctagcgtca tttcattccg catccgtcag atgcatgcgc
     2041 ggatgcattc gtcatgctcc gcccggcagc gcgccgagcg accatcgtct tctcccccaa
     2101 gccgcgactg cgctcgacgc tcagcaatcc cgctgcttgc gcaggcaggc cctgaagcag
     2161 tcctcgcgcc gcgggccggc agggaaaaac tggtcgcagg agtcgtgaca ctgctccggc
     2221 gagcacgcga gccgctggag gggttttgcc gcgccatttg gccggacgcc cgcttccgga
     2281 atgatgacgc cgggcacatt gttcccatgg ctcgtttcca gcgtcgagtt ggcccgcgac
     2341 g
//
LOCUS       DATASH010000268         1728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2213183,
            whole genome shotgun sequence.
ACCESSION   DATASH010000268 DATASH010000000
VERSION     DATASH010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2213183"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VHV99_07395"
     CDS             complement(<1..295)
                     /locus_tag="VHV99_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3635299.1"
                     /translation="MTVHPLQLDHLVIAARTLEEGADFIAAKLGVEMTGGGAHPLMRT
                     HNRLLNLLGGAYLEVIAIDPNAEPVQTPRPRLFALDDPAQIKRLEAGPQLVHWV"
     gene            643..>1728
                     /locus_tag="VHV99_07400"
     CDS             643..>1728
                     /locus_tag="VHV99_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX3635300.1"
                     /translation="MSVPLDQIPAPHGAPQLTLVDQLVQWGRRRIDERVFRPGMRMPS
                     IRKLAVDKGVSRFTVVEAYERLVAHGYLDSRRGSGFFVRDRTSSNDSHVHPTLATERA
                     PVPSTIDVVWLLRNMLHTSSPAKGPGIGYLPASWLNGELLTSALRSMSRQSGAQMLGF
                     GTPQGFLPLRQQIQTRLEELEIGASTEQIVMVSGVTQAIDLIARVFVKPGDTVIVGDP
                     AWFQMFGRFVAQGARLVGMPYTPDGPDLDALEAMVQTWRPKMLVINSVLQNPTGTSLT
                     AAQAFRILQLAETYDFIVVEDDIYGDLCPTNFPATRLASLDQLKRVIFLNSYSKTLAP
                     NLRVGYVAAAPEVANAIADQKMLVGMTS"
BASE COUNT          322 a          556 c          536 g          314 t
ORIGIN      
        1 cgacccaatg cacgagttgc ggcccggctt cgagacgctt gatctgcgcc gggtcgtcga
       61 gtgcaaagag gcgagggcgt ggcgtttgga ctggctccgc gttcgggtcg atcgcgatca
      121 cttcaagata cgcaccgccc aacaggttca gcaggcggtt atgcgtgcgc atcagcggat
      181 gcgcaccgcc tccggtcatc tcgacgccca gtttcgcggc gataaagtct gcgccttctt
      241 ccagcgtgcg agcggcgata acgaggtggt cgagttgcag cggatgaacg gtcatagtgc
      301 gcgaatagct tgaaggaaaa ttgcccggac aacgccggct gagctcgtca gcatacccgt
      361 tccgcccggc cgtgccgctg cgccagctcc gcgttagcca tctggccgag tgtttggccg
      421 gacaatcagc gcatggccaa ggcagcagcc gagagccgtc ccggagctcc tctgcggcag
      481 tgaagcgccc gaaaacgttg cccgaaacgc ctgcagaaac cgccaagttt acctatcgtc
      541 ccagtcttaa atgtatcggc aacgaccgta acagtaacgg tacaatcgat ccgatactct
      601 acggtacagt catcgaacgc atttaacccg atggaacctg acatgtcagt cccgctcgac
      661 cagatccccg cgccgcacgg cgcgccgcag ctgacgctcg tcgaccagct ggtccagtgg
      721 ggccggcgcc gtatcgacga gcgggtgttc cgtccgggca tgcgcatgcc gtccatccgc
      781 aaactcgcgg tggacaaggg cgtgtcgcgc ttcaccgtgg tcgaggcgta cgaacggctg
      841 gtcgcgcacg gttatctcga ctcgcggcgc ggttccgggt ttttcgtgcg cgaccggacc
      901 agttccaacg attcccatgt ccacccaacg ctggccaccg agcgcgcgcc ggtgcctagc
      961 accatcgacg tggtctggct gctgcgcaac atgctgcata cgtcgagtcc ggcgaaaggg
     1021 ccgggcatag gctacctccc ggctagctgg ctgaacggcg aattgctgac cagcgcgctg
     1081 cgttccatga gccggcaaag cggcgcgcaa atgctcggct ttggcacgcc gcaagggttc
     1141 ctgccgctgc gccagcaaat ccagacgcgg ctggaggagc tggagatcgg cgcatcgacg
     1201 gagcagatcg tgatggtgtc cggcgtgacg caggctatcg acctgatcgc gcgggttttc
     1261 gtcaaacccg gcgatacggt catagtgggg gaccccgcct ggttccagat gttcggccgc
     1321 ttcgtcgctc agggcgcgcg gctggtcggc atgccgtaca ccccggacgg gcccgatctc
     1381 gacgcgctcg aagccatggt ccagacctgg cgcccgaaga tgctcgtgat caattccgta
     1441 ctgcagaatc ccaccggtac gtcgctgacc gctgcgcagg cgtttcgcat cctgcaactc
     1501 gcggaaacct acgatttcat tgtcgtggaa gacgatatct acggggacct ctgcccgacc
     1561 aatttccccg caacgcgcct cgcgagcctc gaccaactca aacgggtgat tttcctcaac
     1621 agctattcga agacgctcgc gccgaacttg cgcgtcggtt atgtcgccgc ggcgcctgaa
     1681 gtggcgaacg ctatcgccga tcagaaaatg ctggttggga tgacgagc
//
LOCUS       DATASH010000269         9891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6960058,
            whole genome shotgun sequence.
ACCESSION   DATASH010000269 DATASH010000000
VERSION     DATASH010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 9891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9891
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6960058"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VHV99_07405"
     CDS             complement(<1..380)
                     /locus_tag="VHV99_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEX3635301.1"
                     /translation="MLATLEILLPVFGLIFAGFACRRRGVLGPNSASELNRFVVWLAL
                     PALLFDTMARATWQQLYQPGFVATFSIGCVSVFLLILVIRMLSGRHLADASVDAIAAS
                     YPNTGYIGFPLGLIAFGSASLTPTT"
     gene            506..1372
                     /locus_tag="VHV99_07410"
     CDS             506..1372
                     /locus_tag="VHV99_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3635302.1"
                     /translation="MIDIKPLRYFVTLAEARHFGLAAARLNLSQPPLSRQLAALEASL
                     GVTLLERSPRSVTLTAAGERFYADAKAILASVEQAVSNAQAAAHGDAGKLAIGFTMCA
                     AYNVVPGYARAFGAAFPEVALNLREVVSNDLAAQVLAGQIDAAIMFPNVPDKGLATRT
                     ILREPLCVALSRSHPRARARRLKIAQLAGEPFVLAVEEVAPTLRATILEHCRSGGFEP
                     DIRFEVQLQQTVLSLVDEGVGVALVPASMRKTQLAGVVFRPLVDAPLIEQVLAWSPAN
                     RNPCLARFLELA"
     gene            complement(1382..2047)
                     /locus_tag="VHV99_07415"
     CDS             complement(1382..2047)
                     /locus_tag="VHV99_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VC0807 family protein"
                     /protein_id="HEX3635303.1"
                     /translation="MKARLRYLSALIVNVALPWLAYRLAFPHYGHSGALAASALPLIA
                     WMSWDLLRYRHFDALSALVLVGIVLSLIATALGGDRLIGAIEEPMVSGMIGVSFLISL
                     TLRRPVVFYLARSTMSREDPRSAASFEKHWHERPSLAAYIRLMTLVWGLGMTGENILR
                     SLIVWHWPNDPRAAFASEVVRYSVYAGLTGWTFWCRRRIKRDSLRYPDDPPSPADSTL
                     ASS"
     gene            2308..2844
                     /locus_tag="VHV99_07420"
     CDS             2308..2844
                     /locus_tag="VHV99_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3635304.1"
                     /translation="MPTRRTLLTTDRLIMRPHTRDDFLDSYAMWSDPEVIRYIGGKPF
                     TREEVWARLLRYAGHWAMLGYGYWVVREKDSGRFLGEVGFADYHREIEPSLLDTPEVG
                     WALDPAVHNRGYATEAVRAALAWADAHWPDGDTACIVAPDNQPSLHVAHKCGYREQLR
                     TTYKGKPTIVLRRAAGAA"
     gene            complement(3061..3801)
                     /locus_tag="VHV99_07425"
     CDS             complement(3061..3801)
                     /locus_tag="VHV99_07425"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX3635305.1"
                     /translation="MEPRRNAFGQPIGAPVPDWKGAKAPSCEPLAGSYCRIEPVDVER
                     HAADLYEAYNSAPDGRDWTYLAVGPFESLAAYREHLTRMAASPDPLHHTVIDLATGKA
                     VGTLALMRIDRANGVIEVGHVTYSPRLKRTRIATEAMFLLMSHVFDDLGYRRFEWKCD
                     SLNGPSRTAALRYGFTFEGIFRQAIVYRERNRDTAWFSVIDSEWPALRPSYARWLRAG
                     NFDADGKQVERLVDLIARQRAAAHESGR"
     gene            3911..5710
                     /locus_tag="VHV99_07430"
     CDS             3911..5710
                     /locus_tag="VHV99_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX3635306.1"
                     /translation="MIEIIGPLANPGVKTRAASAAGNGNATGIGTGIGTGSATAIAAG
                     TGSGTAVGSASAAGNGNATGISTGTGNGSAPAPHSGEKSTPLQKQLIERLQQAILTGR
                     LPAGSLLPSSRMLAAEMGVSRNTVVIAYEHLAAVGYVVADKKGTRVSPLSSPSARGEP
                     RRPSVAPKVAYAARVEQFAATRTHADSTLPLTPGTPALDRFPLTAWRRALERSMERAL
                     PRALGYGNPTGEPALRDAIAAHLRMARGVRCEGSQVVITEGAQEALNLCVRLFTNPGD
                     LAWVEDPGYRGAKAAFNVGDLAVRPMPVDLEGIAVPSDAWRTHPPKLIYTSPAHQYPT
                     GAVLSVARRLELIAQARRCGAWLIEDDYDGEFRHTGEPIASMQGLVEDAPVLYVGSFS
                     KTMFPALRIGFVVLPRSIAAHTAVALQEMLRGGHRLEQLALAHFIESGEFGRHLGRMR
                     RLYRERQQALRDALTTHFAPSQILGGNCGMHLTLRLPPRISDRTLAERALAARLNPRA
                     LSGFALQQREEANGLVIGYGGTAAEQLVSAVRTLARLAREIARESSSGPTSAAPSRRA
                     SGATSGSANGLASRPASASTSASTSRTTAKRAV"
     gene            5935..6267
                     /locus_tag="VHV99_07435"
     CDS             5935..6267
                     /locus_tag="VHV99_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635307.1"
                     /translation="MKEIEPTAWFELAAHTPVREGWYEVQLTSGETAFAKYGDGVWTE
                     KPLLVFTHWRGLSADPSKAAEGEAESIGAEATAAQGVRAAWNAFFPGLGEEQHKPLDA
                     VPNGKAPH"
     gene            complement(6421..6906)
                     /locus_tag="VHV99_07440"
     CDS             complement(6421..6906)
                     /locus_tag="VHV99_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3635308.1"
                     /translation="MTIHIRPAHTSDAALILRFITELAVYEKAEHEVVATVQDIETSL
                     FAASSATRALICEMNGEPIGFCVYFFSYSTWLGKHGLYLEDLYVSPSSRGSGAGKQML
                     RHLAQIACESGCGRFEWSVLDWNEPAIGFYESIGASAQSEWVRYRLAGEALTAFADGN
                     R"
     gene            7388..7699
                     /locus_tag="VHV99_07445"
                     /pseudo
     CDS             7388..7699
                     /locus_tag="VHV99_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018310319.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
     gene            complement(7901..9076)
                     /locus_tag="VHV99_07450"
     CDS             complement(7901..9076)
                     /locus_tag="VHV99_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012339835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX3635309.1"
                     /translation="MQMIKSGFSAAVLLLWAGTSYAQSSVTLYGIIDTAVIYGNNEAT
                     GQPGVGHPGVEMNSGGISGSRFGFRGTEDLGGGLAAIFDLEDGFSSANGKLSNTGDIF
                     GRQAYVGLRSTQYGTVTLGRRYAFMSEWVSPLSAEGIGWGGNLADHPFDNDDMIRHQS
                     VNNSIRFDSVNYKGFQAGGMYGFSNEAGQFSNNRAYSFGTKYANGPVSLAAAFEQVDR
                     SASATDLNTNGAESSDDSVINGGREQIWGVGGKYSFGASSVGFVWTHSAFNNVTSAYG
                     EGGAFPSGEDNNLRFDNFEINGRYFVTHALSLGASYTLTDGHFDSGSVHLDPKWHEAI
                     AQADYSFSRRTDVYLESLYQVVTGGGGMALFNASMFNVPPSANNRQLLLVMGMRHRF"
     gene            9437..>9891
                     /locus_tag="VHV99_07455"
     CDS             9437..>9891
                     /locus_tag="VHV99_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013699775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HEX3635310.1"
                     /translation="MLTKTSPAPLAELDAVTLDAQYFEYTSSANPIGAKLISRVPFRN
                     FSPSLYATGETRVVPLDLSAELGCPSPATGPGLCANFLRINAGDALTLAPNATSQLCY
                     VISGTGNVTQGETRFPFWQGDFFTLPGGDAAVLSADTTATLYYVNDAPLL"
BASE COUNT         1850 a         3113 c         3127 g         1801 t
ORIGIN      
        1 gtggtcggcg tcaggctggc tgagccgaac gcgatcaggc cgagtggaaa gccgatatag
       61 ccggtgttcg gatacgacgc cgcgatcgcg tcgacgctcg catcggccag atgccggcca
      121 ctcagcatgc ggatgacgag aatcagcagg aacacgctga cgcagccgat cgaaaacgtc
      181 gcgacgaaac ctggctggta gagctgctgc caggtggcgc gtgccatcgt gtcgaacagc
      241 agagccggca gcgccagcca gaccacgaag cggttcagtt ccgatgctga attcggtccc
      301 agcacgccgc gccgacggca ggcaaagccc gcgaaaatca gaccgaatac gggaagaaga
      361 atctcgagag tggctaacat cggtgcaaat aagcgtgggc ggatggagcg atgcgccagc
      421 gtaatggctt gcgttgatac aatccaatac tgtttttggc ctccaacaat accttttttg
      481 catcgtcgtt cagcctgagg gcgtcgtgat cgatatcaaa ccgctacgct acttcgtgac
      541 actggccgag gcgcgccatt tcggcctggc ggcggcgcgc ctgaatctgt cgcagccgcc
      601 gttgagccgg caactggcgg cgctggaagc gagcctgggc gtgacgctgc tcgagcgcag
      661 tccgcgcagc gtcacgctga cggcggcggg cgagcgcttt tacgccgatg cgaaggcgat
      721 tcttgcgtcg gtggagcagg cggtcagcaa cgcgcaagcc gcggcgcacg gggacgccgg
      781 caagctcgcc atcggcttca cgatgtgcgc cgcgtacaac gtcgtgccag gctatgcgcg
      841 cgccttcggc gccgcgtttc ccgaggtggc gttgaacttg cgcgaggtcg tctcgaacga
      901 tctggccgca caggtgctgg ccggccagat cgacgcggcg atcatgtttc cgaacgtgcc
      961 cgacaagggc ctcgccacgc gcacgatcct ccgtgagccg ttgtgcgttg cgctatcgcg
     1021 gagccatccg cgagcacgcg cgcggcgtct gaagatcgcg caactggcgg gcgagccatt
     1081 cgtgctggcg gtggaggaag tcgcgccgac tttgcgcgcg accatcctcg agcattgccg
     1141 ctcgggcggc ttcgaaccgg atatccgctt cgaggtgcag ttgcagcaga ccgtgctcag
     1201 tcttgtcgat gaaggcgtgg gggtggcgct ggtgccggcc tcgatgcgca agacgcaact
     1261 cgcgggcgtg gtgttccggc cgctggttga cgcgccgctg atcgagcagg tgctggcgtg
     1321 gtcgccggct aatcgcaatc cgtgtctggc gcggtttctc gagttggcgt gaggggtggc
     1381 ttcacgaact tgcgagcgtg ctgtcggcgg gcgatggcgg gtcgtcggga tagcgcagcg
     1441 agtcccgctt gatgcgccgt cggcaccaga aggtccaccc cgtgaggccg gcgtagacgc
     1501 tgtagcgcac tacttcggag gcgaacgccg cgcgcgggtc gttcggccaa tgccagacga
     1561 tcaggctgcg caggatgttc tcgccggtca tgccgagacc ccatacgagc gtcatcagcc
     1621 ggatgtaagc ggcgaggctg ggccgctcgt gccagtgctt ttcgaagctt gcggcgctgc
     1681 gcgggtcttc gcgcgacatc gtcgaacggg cgaggtaaaa caccactggc cgccgcaaag
     1741 tcaacgagat caggaacgac acgccgatca tgccggagac catcggctct tcgatcgcgc
     1801 cgatgagccg gtcgccgccc aaggccgtcg cgatcagcga gagtacgatg ccgaccagca
     1861 cgagcgcgct gagcgcgtcg aaatggcggt agcgcagcag gtcccaactc atccatgcga
     1921 tcaacggtag cgccgaggcg gccaatgcac cggaatggcc atagtgcggg aatgcgagcc
     1981 ggtacgcgag ccacggcaag gcgacgttca cgatcaacgc ggataggtag cgcaagcggg
     2041 ctttcatggg cggagcggcg atcagcgtgg cagcgatgca ggagttgcgc acgccgttcg
     2101 ccgacgcgct tatcgcaccg gcgtggcagc acgtgcgtat ccagggagtg tagggcgaag
     2161 cccctttgca cgccagagtt tgcagtgcgg cgcgagcgtc gcctggatcg tggcagtgaa
     2221 cctggtgcat aagaaatctg cgatcatccc aggcacgctt caacgcttct taaccctgaa
     2281 agcgtccacg ctaactattc gctcaccatg cccacccgcc gcacattgct caccaccgac
     2341 cgcctcatca tgcgcccgca cacgcgcgac gatttcctcg acagctatgc gatgtggtcc
     2401 gatcccgagg tgatccgcta tatcggcggc aagcccttca cgcgcgaaga agtctgggcg
     2461 cgtctgctgc gttacgccgg gcattgggcg atgctgggat acggctattg ggtggtgcgg
     2521 gaaaaagata gcggccggtt tctgggcgag gtcggctttg ccgattatca ccgggagatc
     2581 gagccttcgt tgctggatac ccctgaggtc ggctgggcac tcgaccccgc cgtccacaat
     2641 cgcggctacg cgaccgaagc cgtgcgcgcc gcgctcgcct gggccgacgc gcattggcct
     2701 gacggcgaca ccgcctgcat cgtcgcgccg gacaaccagc cgtcgctgca cgtcgcgcat
     2761 aaatgcggtt atcgcgaaca gcttcgcacg acgtacaaag gcaagccgac catcgtgctg
     2821 cggcgagcgg caggtgctgc atgagccgtc gtatgggcgg catcgcctga gtcgcgcgct
     2881 ggccgctggc cggtcgccgc gtgtcgctgg aatcgccgcg ccggccgcgc gatggccgtg
     2941 tgtgggtcga ataagcgcgt ggctgatgct gtcgttcgcg gccaaagcga tgtcaccgtg
     3001 tcgccggaat cctcgctccg accgtgtgat ggccgcgtgc cggtcagcca ggcggctgtc
     3061 ttatcgaccg ctttcgtgcg ctgctgcgcg ctgccgtgcg atcaggtcca ctagccgttc
     3121 aacctgcttg ccgtctgcat cgaagttgcc ggcgcggagc cagcgcgcgt agctcgggcg
     3181 cagcgcgggc cactcgctgt cgatgacaga aaaccacgcg gtgtcgcgat tgcgttcgcg
     3241 atacacaatc gcctgccgga aaatcccttc gaacgtaaag ccatagcgca acgccgctgt
     3301 gcgtgacggt ccgttcagcg aatcgcactt ccattcgaag cggcgatagc cgaggtcgtc
     3361 gaagacatgg ctcatcagca ggaacatcgc ttcggtggca atgcgggtgc gcttcaggcg
     3421 cggcgaatag gtgacgtggc cgacttcgat cacgccgttc gcgcggtcga tccgcatcag
     3481 cgcgagcgtg ccgactgcct tgccggtcgc cagatcgatc accgtgtggt gcagcggatc
     3541 aggcgatgcg gccatgcgcg tcagatgctc gcgatacgcg gcaaggctct cgaacggccc
     3601 gaccgccaga taggtccagt cgcggccatc gggtgccgag ttgtaggcct cgtacaagtc
     3661 cgccgcatga cgttcgacgt ccaccggttc gatgcggcaa tagctgcccg ccaacggctc
     3721 gcagctgggc gccttggcgc ctttccagtc cggcacgggg gcgccgattg gctgcccgaa
     3781 ggcgttacgt ctcggttcca tgtctgcttc tccagtcgat cgttgttgat ggttcgttgc
     3841 ggagaacaat acggcaaacg tggtatgtta aaaagatcca accgaagcca atttgatagg
     3901 tccagccgcg atgattgaaa tcattggccc gctcgccaac ccgggcgtca agaccagggc
     3961 agccagcgcc gccggcaacg gcaacgctac cggcatcggc actggcatcg gcaccggcag
     4021 cgccaccgcc atcgccgccg gcaccggcag cggcaccgcc gtcggcagcg ccagcgccgc
     4081 cggcaacggc aacgctaccg gcattagcac cggcacagga aacggcagcg cccccgcccc
     4141 ccacagcggc gaaaaatcca cgccgctgca aaagcaactc atcgaacgtt tacagcaagc
     4201 gatcctcacg gggcgtttgc ccgcgggatc gctgctgccg tcgtcgcgga tgctggcggc
     4261 ggagatgggc gtgtcgcgca acacggtggt gatcgcgtat gagcatctgg ccgccgtcgg
     4321 ttatgtcgtg gccgacaaaa agggcacgcg cgtgagtccg ctctccagcc cttctgcgcg
     4381 cggtgaaccg cggagaccct cggtcgcacc gaaagtcgcc tacgcggcgc gcgtagaaca
     4441 attcgcggcg acccgcaccc atgccgacag caccttgccg ctgacaccgg gcacgcccgc
     4501 gctggaccgc tttccactga ccgcctggcg gcgcgcactc gaacgttcga tggagcgtgc
     4561 gttgccgcgc gcgctcggct acggcaatcc gacgggcgaa ccggcgttgc gcgatgcgat
     4621 cgccgcgcat ttgcggatgg cgcggggcgt gcgctgcgag ggctcgcagg tggtgattac
     4681 cgaaggcgcg caagaggcgc tgaacctgtg cgtgcggctc tttaccaatc cgggcgacct
     4741 cgcctgggtt gaggatcccg gctatcgcgg cgccaaggcc gccttcaatg tcggcgatct
     4801 ggccgtgcgg ccgatgccgg tggaccttga aggcatcgcc gtgccatccg acgcgtggcg
     4861 cacgcatcct cccaagctga tctacacgtc gcccgcgcat cagtatccga ccggtgcggt
     4921 gctgtcggtg gcgcgtcggc tggagctgat cgcgcaggcg cgccgctgcg gcgcctggct
     4981 gatcgaagac gactacgacg gcgagttccg ccataccggt gagcccatcg ccagcatgca
     5041 agggctggtc gaggacgcgc cggttttgta cgtcggctca ttcagcaaaa cgatgtttcc
     5101 ggcgctgcgc atcggcttcg tggtgctgcc ccgcagcatc gccgcgcaca cggcggtcgc
     5161 gttgcaggag atgctgcgcg gcgggcaccg tctggagcag ttggcgctgg cgcatttcat
     5221 cgaaagcggc gaattcgggc ggcatctggg ccgcatgcgg cgtctctacc gggagcgcca
     5281 gcaggcgctg cgcgatgcgc tcaccacgca tttcgcgccg tcgcagattc tggggggcaa
     5341 ttgcgggatg catctgacgc ttcgtctgcc gccgcgcatt tccgatcgga ccctcgccga
     5401 acgtgcactc gccgcgcgac tcaatccgcg cgcgttgtcg ggttttgcat tgcagcagcg
     5461 cgaggaggcg aacgggctgg tgatcggcta tggcggcacg gccgccgagc aattggtgag
     5521 cgccgtacgc accttggcca ggctggcgcg ggagatcgcg cgcgagtcga gcagtgggcc
     5581 gacgagcgcg gcgccatcca ggcgggcgag cggggcgaca agcggctcgg caaacggatt
     5641 ggcgagcagg ccggcgagcg cctcgacaag cgcctcgaca agcaggacaa ccgccaaacg
     5701 cgccgtgtaa gtttttacgt ttgtccgcgc ggcccatgtc gcgctatcgt gtcgtttatg
     5761 cgcggcgatt cggaagtgca cgacgttgac gaaccgtccg caggtcatgc ccggcgcacc
     5821 gcgacgcgac atcgcgtgac atgcggcgcg cgcttgcggg gccgctacct ggctcgggca
     5881 tgaagaatgc ctttgttgtg gttatctatg acgatttatc cgaccggaga cgacatgaag
     5941 gaaatcgaac caaccgcctg gtttgagctt gccgcccaca cgcccgtccg tgaaggctgg
     6001 tacgaggtgc agttgacgag cggggagacg gcctttgcga agtatggcga cggcgtatgg
     6061 accgagaaac cgttgctggt gtttacgcac tggcgcggac tttccgcaga cccgtcgaaa
     6121 gctgccgagg gcgaggcaga atcgatcggc gcggaagcca ccgcggctca gggcgtgcgc
     6181 gctgcgtgga acgcattctt cccaggactc ggcgaagagc agcacaaacc gctggacgcc
     6241 gtgcctaacg gcaaagcccc gcattgattg atgcgaagcc tgggcggcgg cttagcggcc
     6301 gccgcctgat gattgcttat ctgccactgt gtttcgaacc acgccacgcg tgtgcaccga
     6361 tcatcttcag cgcgtatggc ggatcacccg accatgcttc tgttcggcgg caagcccgcc
     6421 tcatctattg ccgtcggcaa acgccgtgag cgcctcgccc gccaaccgat agcgaaccca
     6481 ttcgctttgc gcgctcgcac cgatcgattc atagaagccg atcgctggct cgttccagtc
     6541 gagcacgctc cattcgaagc ggccgcagcc gctctcgcat gcaatctggg ccaggtggcg
     6601 cagcatctgc ttgcccgcgc cactgccgcg cgaactgggc gacacgtaca ggtcttccag
     6661 ataaagcccg tgtttgccga gccacgtcga ataagaaaag aaatagacgc agaagccgat
     6721 cggctcgccg ttcatctcgc agatcaaagc cctcgtggca gagctggcgg cgaacaggct
     6781 cgtttcgatg tcttgaacgg tagcgacaac ttcgtgctct gctttttcat acaccgcgag
     6841 ttcggtgatg aagcggagaa tcaacgcggc gtcggatgtg tgggcggggc ggatgtggat
     6901 ggtcaagatc tggtgctcgg tcggtttgta ggatggatcg aggcttggcg agtatgcacg
     6961 cgcatcggat caaacacgca tcgtatcgtc tattctgctg ttctggccag caagcgctgc
     7021 caccctgcga tgtggagatt gcgattgacg tgccttcgaa aaatcgttta ctgtcgatcc
     7081 atggacttcc actcgattcc cttcgctgcc gtcaccacca cgacgaccac cttcacaggc
     7141 gggtcgtctg gaggttgcgc acgctagaag caagaagctg tggcagacca cccaaggccc
     7201 cgccggaaac ggacggggcc tttggcattt ctggacctcc gtttgcggca tcgatccacc
     7261 gtatctgcaa tgtcaagaca aacggagcgt catcgtgaac gacatcgacc atcgtgtact
     7321 gggcaacaaa ctcgacctct tccacctgca ggaagaaggc gcaggcatgg ttttctggca
     7381 cccgcgcggc gcattttacg gcccgaaact ggagtttcat ttaacggaca gccgcgaacg
     7441 ccgctggcaa tgcggcacga tccagctcga tttcgtgttg cccgagcggc tcgacgcgga
     7501 gttcgtcaac gagcgcaatg aacgcgagcg gccggtgatg attcatcacg cggtactcgg
     7561 cagcatggag cgtttcatcg cgatgctgct cgaacatcac gaaggatggc tgccgatgtg
     7621 gctcgcgccc gagcaggtcg tggtcgcgac gatcagcgat gccaatacgg cctacgcgcg
     7681 ggaaatgatg caggcgctcg atgacggcga catacgggcg ggcagcagtc gacgtttgcg
     7741 attgcggaag gtgtcgagta tttaaggctg gctgcgctgc cgcctccatt gcaatcgagg
     7801 ctgtgacggg agcctgtcac cagcggcggc cgcacgtgtg gccgccaacc ggttcacccg
     7861 ccgtcgccgt gccagaccac ggggcgtccc gtccgcaatg tcagaatcga tgccgcatcc
     7921 ccatgaccaa caacaactgc cgattgttgg ccgacggcgg gacgttgaac atcgacgcgt
     7981 taaatagcgc catgccaccg cccccagtga ctacctggta gaggctttcc agatagacat
     8041 cggtgcgtcg gctaaaggaa tagtcggcct gagcgatggc ctcgtgccat tttggatcga
     8101 gatgcacgct tcctgaatcg aaatgtccat cggtcagggt gtacgacgcg ccaagactca
     8161 aggcgtgggt cacaaagtac cggccattga tttcgaagtt gtcgaagcga agattgttgt
     8221 cttcgccact gggaaaagca ccaccctcgc cgtacgcgct ggtgacatta ttaaaggccg
     8281 aatgcgtcca tacgaagccc acagacgagg ccccaaaact gtacttacct ccaacccccc
     8341 agatttgctc ccgaccgccg ttaataacag aatcgtcgct gctctcggcg ccgttcgtgt
     8401 tcaggtcggt agcgctggcc gagcgatcga cctgctcgaa ggcagccgcc aacgacactg
     8461 ggccattcgc gtacttggta ccaaagctat aggcccgatt gttggagaat tggcccgctt
     8521 cgttcgagaa gccatacatg cctcccgcct gaaaaccttt gtaattgacg ctgtcgaatc
     8581 ttatcgagtt attgacgctc tgatgccgaa tcatatcgtc gttatcgaac gggtgatcgg
     8641 cgagattacc gccccatcca atcccctcgg cgctcagcgg cgatacccat tcggacatga
     8701 aagcgtagcg ccgcccgagg gtgacggtcc cgtactgtgt cgaacgcaag ccgacatagg
     8761 cctgccgccc gaagatgtcg cccgtattgc tgagtttgcc attggcgctg gaaaagccgt
     8821 cctccagatc gaaaatagcg gcaagtccac cgccaagatc ctccgttccg cgaaagccaa
     8881 atcgtgaccc actgatacca ccgctgttca tctcgacacc ggggtggcct acgcctggtt
     8941 gtcccgtagc ctcgttgttc ccataaatca ccgcagtatc gatgatgccg taaagcgtca
     9001 cgctgctttg tgcataactg gttcccgccc agagaagtaa aacagccgcc gaaaaacccg
     9061 atttaatcat ttgcactttc aagattcgta gttcaatgca taaacggtta tcgctatata
     9121 ttattttata taacgataac aataaaaaaa ggcgcttgcc gcctgtgcgt cctcaaagaa
     9181 tctgggccgt ctaaatcgta gctgatatgt tgatttgaac ggggtaacag ttcgccgcca
     9241 atgccgggat cacagtgcaa tcctttgcat cgacaattgg cgtcagccaa cccggtgcac
     9301 ccatttgcgc aaaggtgatt tcgatggccg gcacttctga ggccagaggc ccgcacctat
     9361 agtcggttca gcttccagac gcgaacccca acttgcgggg cccacgcgaa gcctccccaa
     9421 tcttaaggac cagaccatgt tgaccaaaac gtccccggcg cctctcgccg aactcgatgc
     9481 cgttaccctc gacgcgcaat acttcgaata tacgtcgtcg gccaatccga tcggcgcgaa
     9541 gcttatctcg cgcgtgccgt tccgcaattt ctcgccttcg ctctacgcaa ccggcgaaac
     9601 ccgcgtcgta cccctcgacc tctccgcgga gttgggttgc cccagtcccg cgaccggccc
     9661 cggcctgtgc gccaatttcc tgcgcatcaa cgcgggggat gcgttgacgc tcgccccgaa
     9721 cgcaacctcg cagctttgct acgtcatcag cgggaccggc aacgtgacgc agggcgagac
     9781 ccgcttcccg ttttggcagg gcgatttctt cacgctgccc ggtggcgatg ccgccgttct
     9841 gtccgccgac acgacggcca cgctctatta cgtcaacgac gcgccgctgc t
//
LOCUS       DATASH010000270        22694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1620575,
            whole genome shotgun sequence.
ACCESSION   DATASH010000270 DATASH010000000
VERSION     DATASH010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 22694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22694
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1620575"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1372
                     /gene="tssF"
                     /locus_tag="VHV99_07460"
     CDS             <1..1372
                     /gene="tssF"
                     /locus_tag="VHV99_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007738555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HEX3635311.1"
                     /translation="AWETVLWPVKISHAAFGDDATLRLTLECEAGVDFSELELTQLRV
                     HLHGDWVVTMPLYELLVAGVTGVSLRPAQGAASTLAPSAWREVGYAPEDHVLPQPSHA
                     HPAYSLMQEYFAFPRKFHFFDLMVPAGRFGTGRTCEIVLHLNRAPRGLGMLGAQHFRL
                     GCTPIVNLFSQTSEPIVIDRRHYEYRLVADQKREAITEVHSIQSVIASDPAKDRATTL
                     PCFTAADRVGNAQSADSPMFWSVRREQSVRSNAPGVDMYLGFVDAGNVAAQPDVPVVY
                     ANLLCTNRRLAEHVPVGARMLVERVSQNARVRCLYEPTAQRNPPLDSATLWRLVSLLT
                     RNYHSLVGGATGRRELQEMLRLFASDGPREEEQIRGIRSINARHVTAHVGSDAWRGYC
                     RGTEVSIEFDADMFVGGSPLLMGAVLARFFAMYTSVNSFVRLVVRRGDEIWKQWEPMT
                     GCQQTL"
     gene            1327..2508
                     /gene="tssG"
                     /locus_tag="VHV99_07465"
     CDS             1327..2508
                     /gene="tssG"
                     /locus_tag="VHV99_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HEX3635312.1"
                     /translation="MEAMGADDRLPADALIARLIAQPQRFDLFQAIQLLERAQPWTRA
                     LGHGDGWNEAVRLKGYVSLAFEASDVRSVERHAAEPVQARADIVDSDFSEADVGEADT
                     SDTDVAAANTYAPRETYTLTTPVMTLAGANGPLPMPFTELVLNRRAARETAMADLLDI
                     FNHRFLSFLYRSRQKVAPGLQSCSPDSAALSACLDALGNLGLRDAPRGPGGARLWLRH
                     AGLLGGAPRSMSGLLALISDRLGVKVRGAQFVGGWRDVDGADSVRLSRVGADSGAARL
                     GGVAVLGRRTWDQAAGISIEFVDLDETTFTALLPGGDAHELVGWLVRNYLQQDHEVFF
                     VLHRRRRAAGFALGGPAAARVGWTSWLGGHPDTASAPAASHRRPAPVRLKMRAVGAVN
                     T"
     gene            2554..5253
                     /gene="tssH"
                     /locus_tag="VHV99_07470"
     CDS             2554..5253
                     /gene="tssH"
                     /locus_tag="VHV99_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007738552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HEX3635313.1"
                     /translation="MDIDIRTLLSRLNPVCKLAMEQAAQLCVRHTHYNVDIEHLLLQL
                     VESDAPDLKAILEHFDVRAADLTAQLQRAVDGFKRGNGRTPALSPNFSPLFQEAWLLS
                     SMLLGEQQIRSGTIVLALLEVDSLRGMLLDSAPALLKIARASLRDALPTLLAASAEGG
                     TSATATATGAGAKTAGAAGPQAAMPSPDANGGRPSALAQFTIDLTAQAREGSIDPIYG
                     RDAEIRQLIDVLLRRRQNNPILTGEAGVGKTAVVEGFAQRIVLGQVPPALANVSVRSL
                     DLALLQAGAGVKGEFENRLKSVIAEVKASPVPVILFIDEAHQLIGAGGSEGQGDAANL
                     LKPALARGELRTIAATTWAEYKKYVERDPALARRFQVVKVEEPAEAVAIEMLRGMVAK
                     LSAHHGVEILDEAVRDAVKLSQRYISGRQLPDKAISVLDTACARVAIGQNGVPEEIEA
                     LGRAIDAAENQLRILRHEAATGAERADAIATLGRQLDDQRAQHARMSDKLGTERQAVG
                     EILAWRRKIAEHVAGTAPTVDDDDSASLAANLSRLEKGLEAVRSDEPMVPVCVDSEMV
                     AKVISGWTGIPVGSMLADELHTVLYLHEKLAERVVGQDEALDAIARRVRTFRADLDDP
                     GKPVGVFLLIGPSGVGKTETAAALADMLYGGERNMITVNMSEFQEAHSVSGLKGAPPG
                     YVGYGRGGVLTEAVRRRPYSVVLLDEMEKAHPDVLELFFQVFDKGVMEDGEGVPIDFR
                     NTLILLTSNAAQDVITEASQGGRRPPPAELIERLRPALLKQFSPAFLGRLVLVPYYHL
                     GDAQITAIVNLKLARLADRFERNHHAPLTWDDAFARTITERCEEVDSGARNVDHILTQ
                     SVLPELAREVLERISVAETFGSVHLGLDAHGGITFHFAPREDQ"
     gene            5255..7144
                     /gene="tssI"
                     /locus_tag="VHV99_07475"
     CDS             5255..7144
                     /gene="tssI"
                     /locus_tag="VHV99_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012405208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HEX3635314.1"
                     /translation="MPMSQSGCLLSITSPFGTDELLLEGFGGSEAISAPFRFDLRMRS
                     TDTALNPQTIVGKTVTVTLSGASGSERYFSGIITRFAQVGANAQYGYYSAELAPRLWL
                     LSLGQDRAIYQNMSALDIIQKVLSTFSIVIESRLSGSYAVREYCVQYDESPLHFISRL
                     MEEEGIFYFFTFANGTHTMVLADSPSAHQPGACPTLRFSSDSVAHTASERVSRFEMST
                     GLVAAELIVADYDYTTGNTSNGMASGQTNGATGTHYVFPGRYATASAGPNAAALQLTA
                     QQVDQQSGHGSSACFVLAAGTQVTLAGHANSGLNAQYVVRRVDHRATNDIYENDFTVF
                     PSQTAFRPPRVSARPVVAGTHTALVTGSSGEEIWTDSLGRIKVKFHWDRVSAADENSS
                     CWVRVAQSSAGAGWGHLFLPRVGQEVVVSYVDGDPDRPLVTGSVYNGQMSPPVTLPGS
                     QTQSVMRSRSSKSGTAGNEIRMEDKINNEELYFHAQKDMNVAIENALTTTVIAGDETH
                     VVKAGNRTVTVSTGNETHTVKGTRSVDVTGDETHTSHAAFSHTTSGNYTLKVSGNLVI
                     DVSGSISIKAGTSLANQAGTTLDNKAGTTLTNQGLEVQNKASASQTVDGGAMLTLKGG
                     LMKLN"
     gene            7171..7908
                     /locus_tag="VHV99_07480"
     CDS             7171..7908
                     /locus_tag="VHV99_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HEX3635315.1"
                     /translation="MTDFLATPPASAALSIESAGAGATTCVEQFDAAGQCVSRTAMLA
                     GVPHGETVRYGEGGARVFQANYVGGKLNGAVRVFDESGVLVQESHYEAGVLHGLTTLH
                     QNGRLLARRHYALGVLQGESLSYAPSGLVSAKQFYDAGKLSREAVFLHEGVVVRRANY
                     RNGQLEGETRDYAADGSLVQSAPYQRDLLHGTVRRFAPDGQVASERRYAHGKPVGAWQ
                     SPIVSAPQTGDKGAPRMVRQFEKWVRG"
     gene            7911..8297
                     /locus_tag="VHV99_07485"
     CDS             7911..8297
                     /locus_tag="VHV99_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4280 domain-containing protein"
                     /protein_id="HEX3635316.1"
                     /translation="MGMQVTAGTTIQCSFGAAPSALTVLPANRVLAGAPAATIMDHAP
                     MVNVMPFGMCSCIANPMVAAATAAALGALTPMGCIPMTSAPWVPGSPTVLIGNFPALQ
                     DSSKLMCMWGGVISVVAPAQFTVQVP"
     gene            8334..8615
                     /locus_tag="VHV99_07490"
     CDS             8334..8615
                     /locus_tag="VHV99_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011617860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635317.1"
                     /translation="MQYVFSMTSRMVTIALACALLLCVLLFLLGFEIGARFVGPSSAT
                     PALAVPSVMGVAPSATTAPAAPAAPIAPAVTTDPAQPGSSTPVPDSSNY"
     gene            8630..9634
                     /locus_tag="VHV99_07495"
     CDS             8630..9634
                     /locus_tag="VHV99_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3635318.1"
                     /translation="MSFPSFTFAQQQQSSSRTARVLRGLLRWSTALAAALVAALAVGL
                     TFGSAPVYAAAPASVPLTPVVAPVPANPIANLPVTSTGLVKLPDGSVLTPEFARIAMR
                     GELVVAVLGVDQPPFFQQQNGQLVGIDIDIAKELANKLGVKVRFNRDAKTFDAVVTLL
                     ANGDADLAISKLSRTLTRAEVVSFSTPYIRLHRALLLNRVKFAQLAKGRAVPDVIRSY
                     DGDIGVVTNSSYATYVQTNFPHAKVQSYPSWEALLKALNAGEVTAAYRDEFEVKHVLK
                     MDPTASLRLRVVTLEDLDDTISIGVNVNAPALLAFVNQFLAERTNKIDVMTLLQASND
                     "
     gene            9652..10926
                     /locus_tag="VHV99_07500"
     CDS             9652..10926
                     /locus_tag="VHV99_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007584414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HEX3635319.1"
                     /translation="MNSKKIYALAVNPWAVIGSLALGGAFGLMLPELSQKFAIVGDIY
                     VNLLKMTTLPFMVSAVIFSLQRLFRDGGTSKLLLRVVTVFIGASVTVALVGAIVLLVM
                     RPGVNLSSATMHTFGLMVGNDTTSNETVMNLYGTDAEQKGVSLYDVLTSLVPTNIFAA
                     LANGDALKALVFALLFGLAVGRVPERISIGLSQALETVYHACQKLMHWLSYPLPLILF
                     CMSAAQLGKSGVGPLHAMLQFVIALFVASVVLVALAAIVIWKRSSHSLGKTLDALRAP
                     FALALATRNSAACMPSMIESLVDDLGFARARVELMVPLTVSLLRVGPMVYYVCATLFI
                     AQLYGHTLGFVEVCTVLLASVLAGFASAGMTGLVTVSLIGMTCAYLSLPFEAAFILFL
                     AVDPLCDMLRTLILVIGNTAAVTVICPRPLKI"
     gene            10942..11652
                     /locus_tag="VHV99_07505"
     CDS             10942..11652
                     /locus_tag="VHV99_07505"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007584416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HEX3635320.1"
                     /translation="MALIGILGGMGPLATVDFFERVIELTSSAGASCDQEHLPLLLAN
                     LPHIPDRSKAMLKTGENPLPALLDGIDMLNRNGVELIAIPCNSAHFWFDAMREHSRAP
                     ILHIAEACVAAIPPGTRAAAVLATGGTLLSGLYQRILERHGIEPVVPNETTQKHIDAC
                     IQAVKAGSLDLAAFELQAALAEFAARGAEAAVMGCTEIPLAARRIASPSMQLIDSTLE
                     LARATVSYGLARGWNARQ"
     gene            11649..13067
                     /locus_tag="VHV99_07510"
     CDS             11649..13067
                     /locus_tag="VHV99_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HEX3635321.1"
                     /translation="MKLLKRLSNSTLILLLCFALGGLAGTFAAPIGQFAFFIGQIYLA
                     LVSMAAVPLLVVAMFFGLRQLMLLSHPGLRISTMLTLSLGLVFAAALVGMLTGMVVAP
                     GLHLSDAAHRQLGQLVLQSASDAEDMRMKLFGTDSAGAASSGLALRDVVPDNFYRVLA
                     GGHALGILTGTILFGMAFAALSGEQTRVLHGVFESIYRSLETLIEYANLLLPVLAFGT
                     AAHLVEQTEHETLDAMSGFLVSFLVCTAVLSTVIVVLIAARARASLVRVAAALRAPML
                     VGLTSGNASATIPHTIEAMSTRLGFSRGVAELVVPFGSVFVRAGSALYFTLATVFIAN
                     LYDRPLGVGELLLTAAAATVAAFASAGRNGVASISYVGIVLSLLHLPVEAAGVLLIAV
                     DLLCDGPRNLLSLLSVCTVVALVSAGLPAERAAAPEHGVRPEAAAVLRFTFTRGQLVL
                     ATGCLVAAASLIVLMGVGVGAK"
     gene            13064..13591
                     /locus_tag="VHV99_07515"
     CDS             13064..13591
                     /locus_tag="VHV99_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635322.1"
                     /translation="MNKVQAILNSGRRGFTWATALGRLRVACFALCVASLVSGCGSFL
                     SSKGEKVRWDHVTLVADDDVNNNSPVAVDVVFVTDDAMLARIAELPASKWFASRADLA
                     NTFPKSLQYKSWELVPGQRIEVSGDSFGRPRVEAAFLFANYTEPGAHRMRIEKLSGNL
                     AVQLNTYTFTVSAVK"
     gene            13688..15019
                     /gene="tssK"
                     /locus_tag="VHV99_07520"
     CDS             13688..15019
                     /gene="tssK"
                     /locus_tag="VHV99_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010810720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HEX3635323.1"
                     /translation="MFEQMTPVVDRIEWHEGLLLSPQHFQQMSARVDSLVAWQTLAAA
                     PFSWGVRRLVLDAGLLPAGTLRVLALEAIMPDGTSVSYSADSSQQGRLDLSLEPYAEQ
                     LAKGPLDFYLTLPIAASMRRDGESRRFRSTLGAPVEDEISDAPPADVPRLIPNLGLTA
                     GELPAASFVSLRLGSIYRDNEVVRLAERLPPLIEISRDTPLWTNVATLLGQLRGKAAF
                     VARQTANPSSKVDDRLTQLELKDRLRSLLAALPLVEAVLRTPHLHPLALYHALAALSG
                     SLAMLVPGGLPPVPPDYDHGDPLAVFTPMLRTLRDAVSEVAEGYREHKFEFRHGAFEI
                     VLNAEWIGERIVVGLRGQSDRDLLAWMSGAVIGSQSIYAMLRTRRVLGATRRVVDYAE
                     ELGIRSGSGYLLFEIQADPSLIVTSEALVISNPNEGATAQRPQEMLLFVKS"
     gene            15052..15804
                     /locus_tag="VHV99_07525"
     CDS             15052..15804
                     /locus_tag="VHV99_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012405218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotU family type IV/VI secretion system protein"
                     /protein_id="HEX3635324.1"
                     /translation="MARNRIESEENDLVMMQFRSFLDELVKAQKRFSDASEGDAEQAA
                     SGLSRHLLNLLEVQTLQAHRDSTRFDLENLADARYLKTVLADEILLNTQWAGRGHWTA
                     YLLESTLFRTNHAGDQVFRRIEQLLSDREPSRRDIARICLFALAMGFQGRFRGTDSTA
                     RLRSYREELYEFVYQRRADLGGRDRVLADAAYGNTISHIAPRKLPTVSRWTVIGLLAV
                     AALLAISEMLWLWQSWPVRAALHGDVVAGVNS"
     gene            15842..20044
                     /locus_tag="VHV99_07530"
     CDS             15842..20044
                     /locus_tag="VHV99_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007584425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein"
                     /protein_id="HEX3635325.1"
                     /translation="MLTSNLFLLSLAVLIIVICVVLGAVVYFALHGAHAQPQAERKIV
                     RLRTDSLRSAFRQAVELLEGNIASRAERYNIPWVLVLNEGDDPRPLPIAQSGVASVLS
                     AEAASPAATQGISWQFFDRGIVVDIKAAYLGSPDEEGDEKPWDEFLSLCRNYRPQRPF
                     DSVVITVPASMLLAENTDARLELERHAKLAHRRLWLAQNRFAMRFAVYVVVTGCEALS
                     GFAEFARALPEPLRASMLGWSSPYDLSVTYQSDWVDTALGTIERTVSDASAELFALDA
                     NGLDARECLLLPSRIDAIRAQLQLYVDELLRASAYHEPFFFRGIYLTGDSSELAQRSV
                     VAPALADYAAEGDYDAYGRGPEPLLAGSDSAEEGDAGTAVVVAQERARSDSESYDPAQ
                     TVNDLMLRPAFLRDLFDKKIFLEYGLTRPSRSQHLTRPALHRGLRWGGMALLGVWGVG
                     LVVATVQLNHRNGALVSALDELRKDSQERAAAEQGGVQLPADWYRRKAIALIALNDRL
                     QTGTPWTVFMPGSWSAVDDLNQRVHERFEQEFGAIAVTALEREMLARVGQMTGVARDP
                     ATGQLVVGDDCSAPQTIGSDAPVAASLAPEDQPAMRALQRYVGNVDQLDAALQAMDRL
                     RQPSQDNADALRLAVRYAFGVELQGNVAASLPYFYRNARHGGAYASDATGGINLPAVQ
                     QALRCTFDKGAQQMDDALFTSNPLLATERSITTHLNTLSVADASAADFTQVTNNYRAI
                     AAGIGTQQDLLATGKGAWMRQTQFMPGAIYERTFARAAQSRLLGPDLVARMRERSGTG
                     FQAFRSELALRVGAPDSGIVWTEKGARYDVAPERLALRDGLMKLLNQPFMVAPRGLSL
                     PTLADGSVVAWDRVQLDQALALSDVRKRFLADGLANLPEAVQPSIEQAVDVQFARLIG
                     DQVAAAATVTPAQGEPDGGNFDAARLRLTKIRAALVDMGAAAQIDDLDTLVSRDALAH
                     LRLVDDALNRSELYSTRPAPVSVSGNAASHGMMLAAFGINDVGSLGAYLDQQATRALA
                     LGRQASAYLAALGPGEAGSPLALRWQAIVRDLDRYQLKNPNSSLFALEQFVQTVAADP
                     GAGDCITHFPPRPSGLATGDYFSALHARLYDTLLARCGQGYTDDLRQQWNGFSTAFNQ
                     GVAGHMPFGAPGRGVNGDANTADFSELGQTLRQYERVSHVFQQSRSGAGERVIGSRSP
                     VRQFVDNFDLVDALLAPLYPATDGAPSGLDVSVEFRANRSGEVAANQVIDWTLKIGDQ
                     SVSLHDAPHPLHWEYGMPVSLTLRLAKDSPLASAIDPQQRAYSTDGHTATWTFGDPWA
                     LITFVSRQRVADAGARGANTSQLLKFEFPLTVVNVADEAMLPKQSRGQVYVRLVLSPT
                     GKKTALPWPGSFPNRAPEWSAL"
     gene            20041..21177
                     /gene="tssA"
                     /locus_tag="VHV99_07535"
     CDS             20041..21177
                     /gene="tssA"
                     /locus_tag="VHV99_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HEX3635326.1"
                     /translation="MNAPLLLEDEALTQPDGIGEYLAPLQDGNGVGVSLRHDAVFQKI
                     REARQQDDPTLPMREWERPLIKADWNTVASLSGEALRTRSKDFQLAAWLCEAWTHQRG
                     VEGFSDGMRLLHALVDGFWEHAYPQLEDGDSDARVAPFVWVNQTLAMVLKLQIPLLTL
                     SVEPGSINFDDWTRIVGRVSHDEESEELAREFLDGEVDKGSNLADLIRLEKQLGVALD
                     VTATFERGLDMRLGNEAPSFSKVLEALMDLKRVARSLRGSRAMPMVNEAPEVQVHNEV
                     QSVVTNVADDENNAFVVAPHSPAASDTTSVMTPFRIADRTHAYELLDAVAQYLARTEP
                     HSPTPYLLKRAVAWGGMPLPELMREVVSQEGDLTRYFAMLGLHE"
     gene            21450..22598
                     /locus_tag="VHV99_07540"
     CDS             21450..22598
                     /locus_tag="VHV99_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635327.1"
                     /translation="MPEVNGIPESKEFKERIGRTGISSITKKKDGQMDTILACLQNAG
                     YWRDVPTPNAIAPLRLVRKECISWLVANPPSVTRAARPHITQLLNAVNSRINAVIDRN
                     FVVNDQRTPQQLIQRLKEGFNIGTGLYVADRNGTVTRGGRVGKRLADGYLVERMTSTH
                     MPKMHFEGAFTKYKKANTKLKFETWVQQVLLPQSEDDPMGVYFTADDSRLGKFNQLAS
                     EDRVQYCNEDERKDYVITITAGVVKDASGDLYHTGSKETHFSGHGWAIYVVGMENTFY
                     SNSHLVNKFHHSSFLAGAPVQGAGEIAVNNGKVVAVTNKTGHYSAGPAELTRTLFLLH
                     RGGVNLDEILVNDPFRAANVWITGRDCLLAGGDFNGKVSLPKPATVPA"
BASE COUNT         3930 a         7038 c         7514 g         4212 t
ORIGIN      
        1 tgcgtgggaa acggtactgt ggcccgtcaa aatcagccac gccgcattcg gtgatgacgc
       61 cacattgcgt ctcacgctcg aatgcgaagc gggcgtggat ttctccgaac tcgaactcac
      121 gcagttgcgt gtgcatctgc acggcgactg ggtcgtcacg atgccgctgt atgaattgct
      181 ggtggcgggc gtgacaggcg tgtcgctgcg gccggcgcaa ggcgctgcgt cgacgctcgc
      241 gccttcggcg tggcgcgaag tgggttatgc gcccgaagac catgtattgc cgcagccttc
      301 gcatgcgcat ccggcttaca gcctgatgca ggaatatttc gcgttcccac gcaaattcca
      361 tttcttcgat ctgatggtgc ccgccggtcg cttcggcacg ggccgcacct gcgagatcgt
      421 gctgcatctg aatcgcgcgc cgcgcggcct tggcatgctg ggcgcgcagc atttccgtct
      481 cggctgcacg ccgatcgtca atctgttttc gcagaccagc gagccgatcg taatcgaccg
      541 gcgacactac gaataccggc tggttgcgga tcagaagcgc gaagcgatca cggaagttca
      601 ttcgatccag tcggtgatcg cgtccgatcc cgcgaaagat cgcgcgacca cgctgccttg
      661 tttcaccgct gccgatcgcg tcggcaacgc gcaatcggcg gacagcccga tgttctggtc
      721 cgtgcgccgc gagcagagcg tgcgcagcaa tgcgcctggc gtggacatgt atctgggttt
      781 cgtcgacgca ggcaatgtgg cggcgcagcc ggacgtgccg gttgtctacg cgaacctgct
      841 gtgcaccaac cggcggctcg ccgagcatgt gccggtgggc gcgcgcatgc tggtcgagcg
      901 tgtgtcgcaa aacgcgcggg tgcgctgtct gtatgagccg accgcgcagc gcaatccgcc
      961 gctcgacagc gcgacgctgt ggcggctcgt ctcgctgttg acgcgcaatt atcactcgct
     1021 cgtgggcggc gcgaccggcc gccgcgagtt gcaggaaatg ctgcggctgt ttgcatctga
     1081 cggtccgcgc gaagaagaac agattcgcgg cattcgcagt atcaacgcgc ggcacgtcac
     1141 cgcgcacgtc ggcagcgatg catggcgcgg ctactgtcgt ggcaccgaag tgagtatcga
     1201 gttcgacgcc gacatgttcg tcggcggatc gccgctcctg atgggcgcgg tgctcgcgcg
     1261 cttcttcgcg atgtacacgt cggtgaattc ctttgtacga ctggtggtgc gacggggaga
     1321 cgagatatgg aagcaatggg agccgatgac cggttgccag cagacgcttt gatcgcccgt
     1381 ctcattgcgc agcctcagcg cttcgatctg ttccaggcga tacagctgct cgaacgcgcg
     1441 cagccttgga cgcgcgcgct cggtcacggc gacggctgga acgaggccgt gcgtttgaag
     1501 ggatacgtgt cgctcgcatt cgaagcgagc gatgttcgca gcgtcgaacg tcacgcagcg
     1561 gagccggtgc aggcgcgcgc ggacatcgtt gactcagact tcagtgaagc agacgtcggt
     1621 gaagcagaca caagcgacac ggacgtcgcc gctgcgaaca cttacgcgcc ccgcgaaacg
     1681 tacacgctga ccacgccggt gatgacgctc gccggcgcga acggcccatt gccgatgccg
     1741 ttcacggaac tggtgctgaa ccgtcgtgcg gcacgcgaga cggcgatggc cgatctgctc
     1801 gacattttca atcaccgctt tctgtcgttc ctgtatcgca gccggcaaaa agtcgcgcct
     1861 ggtttgcaga gctgctcgcc ggattccgcg gcgctttccg cgtgtctcga cgcgctcggc
     1921 aatctcggtt tgcgtgacgc gccgcgcggt ccgggcggcg cgcgtttgtg gctgcgccac
     1981 gccggtctgc tcggcggcgc gccgcgttcg atgagcggac tactcgcgct catctccgac
     2041 cggctcggcg tgaaggtgcg cggcgctcaa ttcgtcggcg gctggcgcga cgtggacggc
     2101 gccgattcgg tgcgactgtc gcgcgtcggt gcggattcgg gcgccgcgcg gctcggtggc
     2161 gttgcggtgc tcgggcgtcg cacgtgggac caggcggcgg gcatctcgat cgagttcgtc
     2221 gatctcgacg agacgacatt taccgcgctg ctgccgggcg gcgacgcaca cgaactggtc
     2281 gggtggcttg tgcgcaatta tctgcagcag gaccacgagg tattttttgt tttgcatcgt
     2341 cgacggcgcg cagcggggtt cgcgctcggc ggtccggcgg ctgcgcgcgt cggctggacg
     2401 tcgtggctcg gcgggcatcc cgatacggct tcggcaccgg ctgcatcgca ccgccggccc
     2461 gcaccagttc gactcaagat gcgcgccgtt ggcgccgtta atacataagc cgagacggcg
     2521 cggtttcgcc acgtcacgga cacacggaac gatatggata tcgacattcg aacattgctg
     2581 agccgtctca atccggtatg caagcttgca atggagcagg cggcacagct ttgcgtgcgt
     2641 catacgcatt acaacgtcga catcgagcat ttgctgttgc aactcgtcga atccgacgcg
     2701 cctgatctga aggcgattct cgagcatttc gacgtgcgtg ccgccgatct gaccgcgcag
     2761 ttgcagcgcg ccgtggacgg cttcaagcgc ggcaacggcc gtacgcccgc gctgtcgccg
     2821 aatttctcgc cgctgttcca ggaagcgtgg ctgctcagtt cgatgctgct cggcgagcag
     2881 cagattcgtt ccggcacgat tgttctcgca ttgctcgaag tagacagtct gcgcggcatg
     2941 ctgctcgact cggcgccggc cttgctgaag atcgcgcgcg ccagtttgcg cgatgcgctg
     3001 cctacgttgc tggcggcatc ggcggaaggc ggcacgagcg cgaccgcaac cgcaaccggt
     3061 gcgggtgcga agactgccgg cgcggcaggg ccacaagcgg cgatgccgtc gccggatgcg
     3121 aatggcggtc gtccgtcggc gctcgcgcaa ttcacgatcg acctgactgc ccaggcgcgc
     3181 gaaggctcga tcgatccgat ctacggccgc gacgccgaaa tccgtcagtt gatcgatgtg
     3241 ctgttgcgcc gccgtcagaa caatccgatc ctgaccggcg aggccggcgt cggcaagacg
     3301 gctgtcgtcg aaggtttcgc gcagcgcatc gtgctcggcc aggtgccgcc ggcgcttgcc
     3361 aacgtgtcgg tgcgttcgct tgatctcgcg ctcctgcaag cgggcgcggg cgtgaaaggc
     3421 gagttcgaga accggctcaa gtcggtgatc gcggaagtga aggcttcgcc cgtgccagtc
     3481 attctattca tcgacgaagc gcatcagctg atcggcgcgg gcggcagtga aggtcagggc
     3541 gacgcggcta acctgttgaa gccggcgctc gcgcgcggcg agttgcgcac cattgccgcg
     3601 accacgtggg ccgaatacaa gaagtacgtg gagcgtgacc cggcattggc gcggcgcttc
     3661 caggtcgtca aggtcgaaga gccggctgaa gcggtcgcca tcgaaatgct gcgcggcatg
     3721 gtggcgaagc tttccgcgca tcacggcgtg gaaattctcg acgaagccgt gcgcgacgcc
     3781 gtgaagctct cgcaacgtta tatctcgggc cgccagttgc ccgacaaggc gatcagcgtg
     3841 ctcgacacgg cttgcgcgcg cgtggcgatc ggccagaacg gcgtgcccga agaaatcgag
     3901 gcactgggtc gtgcgatcga cgccgccgaa aaccagttgc gcattctgcg tcacgaagcg
     3961 gctaccggcg cggagcgcgc cgatgcaatc gccacgctcg gacgtcagct cgacgaccag
     4021 cgcgcgcagc acgcgcgcat gtccgacaag ctcggcacgg agcgtcaagc ggtcggcgaa
     4081 attctcgcgt ggcgccgtaa gatcgccgaa cacgtcgccg gcacggctcc gacggtggac
     4141 gatgacgaca gcgcgtcgct cgccgccaat ctctcgcgtc tggaaaaagg cctcgaagcc
     4201 gtgcgcagcg acgagccgat ggtgccggtg tgtgtcgatt cggaaatggt cgcgaaggtg
     4261 atttccggct ggaccggcat cccggttggc agcatgctcg ccgacgaatt gcacacggta
     4321 ctgtatctgc acgaaaagct agccgaacgc gtggtgggcc aggacgaggc gttagacgcg
     4381 atcgcgcgcc gcgtgcgcac gttccgcgcc gatctcgacg atccgggcaa gccggtcggc
     4441 gtgtttctgc tgatcgggcc gagcggcgtc ggcaagacgg agacggcggc ggcgctcgcc
     4501 gacatgctgt acggcggtga acgcaacatg attaccgtga acatgtccga gttccaggag
     4561 gcgcacagcg tctcggggct caagggcgcg ccgcccggat acgtgggtta tggacgcggc
     4621 ggcgtgctga ccgaagcggt gcggcgtcgt ccgtatagcg tcgtgctgct cgacgaaatg
     4681 gagaaggctc acccggacgt gttggaattg ttcttccagg tgttcgacaa gggcgtgatg
     4741 gaagacggcg aaggcgtgcc gatcgacttc cgcaacacgc tgatacttct cacgtcgaac
     4801 gccgcgcagg acgtgattac cgaggcgtct cagggcggcc gccgtccgcc ccccgccgag
     4861 ttgatcgagc ggctgcgtcc ggcgttgctg aagcagttca gccccgcgtt cctcggccgt
     4921 ctcgtgctgg tgccgtatta ccacctgggc gacgcgcaga tcaccgcaat cgtgaatctg
     4981 aaactcgcgc ggctggccga tcgcttcgag cgcaatcatc acgcgccgct cacgtgggac
     5041 gacgcgttcg cgcgcaccat caccgagcgc tgcgaggaag tggacagcgg cgcacgcaat
     5101 gtggatcaca tcctgacgca atccgtactg ccggaactcg cgcgcgaagt gctggaacgg
     5161 atctcggttg ccgagacgtt cggcagcgtg catctgggac tcgacgcgca cggcggcatc
     5221 acgttccatt tcgctccgcg cgaggaccag taacatgccg atgtcgcaaa gcggctgcct
     5281 gctgtcgatt acctcgccgt tcggcaccga cgagcttttg ctcgaaggct tcggcggcag
     5341 cgaggcaatc tcggcgccgt ttcgcttcga tctgcgcatg cgctcgaccg acaccgcgct
     5401 caatccgcaa accatcgtgg gcaagacggt gacggtgacg ctcagcggcg cgtcgggctc
     5461 ggaacgctat ttcagcggca tcatcacgcg cttcgcgcag gtcggcgcga atgcgcagta
     5521 cggctattac tcggccgaac tcgcgccgcg tctgtggctg ctgtcgctcg gccaggaccg
     5581 cgcgatttat cagaacatga gcgcgctcga catcatccag aaggtgctgt ccacgttcag
     5641 catcgtcatc gagtcgcgcc tgagcggcag ctacgcggtg cgcgaatact gcgtgcaata
     5701 cgacgaaagc ccgcttcact tcatttcgcg gctgatggaa gaggaaggca ttttctactt
     5761 cttcacgttc gcgaacggca cgcataccat ggtgctcgcg gacagtccgt ccgcgcatca
     5821 acccggcgcg tgcccgacgc tgcggttttc cagcgactcc gtcgcgcaca cggcgagcga
     5881 gcgcgtgagc cgcttcgaaa tgtcgacggg actcgtcgcc gccgaactga tcgtcgccga
     5941 ctacgactac accacgggca acacgtccaa cggtatggcc agcgggcaaa ccaacggcgc
     6001 gaccggcacc cactacgtgt ttccgggtcg ttacgcaacc gcgtccgctg gcccgaacgc
     6061 cgccgcgctg caactcaccg cgcagcaggt ggaccagcag agcggccatg ggtcgagcgc
     6121 gtgcttcgtg ctggcggccg gcacccaggt gacgctcgcg ggccacgcga acagcggcct
     6181 gaacgcgcag tacgtggtgc gccgcgtcga tcatcgcgca accaacgata tctacgagaa
     6241 cgatttcact gtgtttccgt cgcagaccgc gttccggccg ccgcgcgtga gcgcgcggcc
     6301 cgtggtcgcg ggtacgcaca ccgcgctggt gacaggctcg tcgggcgagg aaatctggac
     6361 cgactcgctc ggccgcatca aggtgaaatt ccactgggac cgcgtgagcg ccgccgacga
     6421 aaacagttcg tgctgggtgc gtgtcgcgca gtcgtcggcg ggtgcgggct ggggccatct
     6481 gtttttgccg cgcgtcggcc aggaagtggt ggtcagttac gtcgacggcg atccggaccg
     6541 tccgctggtg acagggagtg tctacaacgg gcagatgtcg ccgcccgtca cgctgcccgg
     6601 ctcgcagacg cagagcgtga tgcgctcgcg ctcgtcgaaa agcggcacgg caggcaacga
     6661 aatccgcatg gaagacaaga tcaacaacga ggagctgtac ttccacgcgc agaaggacat
     6721 gaacgtcgcg atcgaaaacg cgctgacgac gaccgtgatc gcgggcgacg aaacgcatgt
     6781 ggtgaaggcg ggcaatcgga cggtgacggt gtccaccggc aacgagacgc atacggtgaa
     6841 gggcacccgt tcagtcgacg tgaccggcga cgagacgcac accagccacg ccgcattcag
     6901 ccacacgacg agcggtaact acacgctgaa agtgagcggc aatctggtga tcgacgtgag
     6961 cggttcgatc agcatcaagg cgggcacgtc gctcgcgaac caggcgggca cgacgctcga
     7021 caacaaggcc ggtacgacgc tcacgaacca ggggctcgag gtgcagaaca aggcatcggc
     7081 gagtcagacc gtggacggcg gcgccatgct caccctcaag ggtggcttga tgaagctgaa
     7141 ctgagctcgt agagcgaacc gacccgcgcc atgaccgact tccttgccac gccgcccgct
     7201 tccgccgcgc tttccattga aagcgcgggc gctggcgcaa ccacgtgcgt cgaacagttc
     7261 gacgcggcgg gtcagtgcgt gtcacgtacg gcgatgctgg ccggtgtgcc gcatggcgag
     7321 acggtgcgtt acggcgaagg cggcgcgcgc gtgttccagg cgaattacgt cggcggaaaa
     7381 ctgaacggcg cggttcgcgt gttcgacgaa agcggcgtgc tggtgcagga atcgcactac
     7441 gaagccggtg tgctgcacgg cctgacaacc ctgcatcaga acgggcggct gctggcgcgc
     7501 cgccactatg cattgggcgt gctgcaaggc gaaagcctct cgtacgcgcc gtcgggactc
     7561 gtgagcgcga agcagtttta cgacgcgggc aagttgtcgc gcgaggcggt gttcctgcat
     7621 gaaggcgtgg tcgtgcggcg cgcgaactat cgcaacggac aactggaagg cgaaacgcgc
     7681 gattacgccg ccgacggttc gctcgtgcaa agcgccccgt atcagcgcga cctgctgcac
     7741 ggcacggtac ggcgtttcgc gccggacggc caggtcgcgt cggagcgccg ctacgcgcac
     7801 ggcaagccgg tcggtgcatg gcaaagtcca atcgtgagcg caccgcaaac gggcgacaag
     7861 ggggcgccgc gcatggtccg gcagttcgaa aagtgggtga ggggctaacc atgggtatgc
     7921 aagtcaccgc gggtaccacg attcagtgca gtttcggcgc ggcgccgtcc gcattgacgg
     7981 tgttgcccgc gaaccgcgtg ctcgccggcg cgccggccgc gacgatcatg gatcacgcgc
     8041 cgatggtcaa cgtgatgccg tttggcatgt gttcgtgcat cgcgaatccg atggtggccg
     8101 cggcgacggc ggcggcgctg ggcgcgctga cgccgatggg ttgcataccg atgacgagcg
     8161 cgccgtgggt gcccggttcg ccgaccgtgc tgatcggcaa ctttccggcg ctgcaggatt
     8221 cgtcgaaatt gatgtgtatg tggggcggcg tgatttcggt cgtcgcgccc gcgcagttca
     8281 ccgtgcaggt cccctgagcg gatctgaaca acaggatttc cgagagctac acgatgcaat
     8341 acgttttttc gatgacttcg cggatggtga cgatcgcgct tgcgtgcgcg ctgttgctgt
     8401 gcgtgctgct gtttctgctg ggcttcgaga ttggcgcgcg cttcgtcggg ccgtcatccg
     8461 cgacgcccgc tttggccgta ccgtcagtca tgggcgttgc gccttcagcg accactgcgc
     8521 ccgctgcacc cgctgcaccg atcgcgccag ccgtgacaac cgacccagcg cagccgggct
     8581 cctctacgcc cgttcccgat tcttccaact attgataccg atacccgaga tgtcgtttcc
     8641 gtccttcaca tttgcgcagc agcagcaatc ctccagtcgc accgcccgcg tgttgcgcgg
     8701 cctgttgcgc tggagcacgg cgctcgccgc cgcccttgtt gccgctcttg cagtcggtct
     8761 cacgtttggc agcgccccgg tttatgcggc cgcgccggcc agcgtcccgc tcacgccggt
     8821 tgtcgcgccg gtccccgcga atccaatcgc gaatctgccg gtcacttcca cgggactcgt
     8881 caagcttccc gacggcagcg tgctcacgcc ggaattcgct cgcattgcca tgcgcggcga
     8941 actggtggtc gcggtgctcg gcgtcgatca gccgccgttc ttccagcaac agaacggcca
     9001 gcttgtcggc atcgacatcg acatcgcgaa ggaactcgcc aacaagctcg gcgtgaaggt
     9061 ccgcttcaac cgcgacgcga agaccttcga cgcagtggtg accttgctcg ccaacggcga
     9121 cgccgatctg gcgatcagca agctctcgcg cacgttgacg cgcgccgagg tggtcagttt
     9181 cagcacaccg tatatccgct tgcaccgcgc gctgctgctc aatcgcgtga aattcgcgca
     9241 actggctaaa ggccgcgccg tgcccgacgt tatcagaagc tatgacggcg acatcggcgt
     9301 ggtgacgaat tcgtcttacg cgacgtatgt gcagaccaac ttcccgcacg cgaaagtgca
     9361 gagctatccg agctgggaag cgttgctgaa ggcgctgaat gcaggtgaag tcaccgccgc
     9421 gtatcgcgac gaattcgaag tcaagcacgt gctgaagatg gacccgacgg cttcgctgcg
     9481 tctgcgggtc gtcacgctgg aagacctcga cgacacgatt tcgattggcg tgaacgtcaa
     9541 cgctccggcg ctgctcgcgt tcgtcaacca gtttctcgcc gaacgcacca acaagatcga
     9601 cgtgatgacc ctgctgcaag cgtccaacga ctaatcggct tcgaaccaac aatgaactca
     9661 aagaaaattt acgcgctggc cgtgaaccct tgggcggtga tcggcagtct cgcgctaggc
     9721 ggcgcgttcg gtctgatgct gcccgaactg tcgcagaagt tcgcgattgt cggcgacatc
     9781 tacgtgaatc tgctgaagat gaccacgctg ccgttcatgg tgtcggcggt gatttttagt
     9841 ctgcagcgcc tctttcgcga cggcggcacg tccaaactgc tgttgcgcgt ggtgacggtg
     9901 ttcatcggcg cgtcggtgac ggtcgcgctg gttggcgcga tcgtgctgct ggtgatgcgc
     9961 cctggcgtga atctctccag cgccaccatg cacacgttcg ggctgatggt cggcaatgac
    10021 acgacctcga atgaaacagt gatgaatctg tacggcacgg acgccgagca gaaaggcgtg
    10081 agcctctacg acgtactgac gagcctcgtg ccgaccaaca tcttcgcggc gctcgccaac
    10141 ggcgacgcgc tcaaggcgct ggtcttcgcg ctgctgttcg ggctcgcggt tggccgcgtg
    10201 ccggagcgca tttcaatcgg cttgagccag gcgctcgaaa cggtctatca cgcatgccag
    10261 aaactgatgc actggctgag ctacccgctg ccgttgatcc tgttctgcat gagcgcggcg
    10321 cagttgggca aatcgggcgt cggtccgcta cacgcgatgc tgcaattcgt gatcgcgctc
    10381 ttcgtcgcgt cggtggtgct ggttgcactc gcggccatcg tgatctggaa gcgttcgagc
    10441 cattcgctcg gcaagacact cgacgcgctg cgcgcgccgt tcgcactcgc gctggccacg
    10501 cgcaacagcg cggcctgcat gccgagcatg atcgaaagtc tcgtagacga cctgggcttc
    10561 gcccgcgcgc gcgtcgagct gatggtgccg ctcacggtgt cgttgttgcg cgtcgggccg
    10621 atggtgtact acgtgtgcgc gacgctgttc atcgcgcaac tgtacggcca cacgctcggc
    10681 ttcgtcgaag tctgcacggt gctgctcgcc tcggtgctgg ccggtttcgc ttcggcgggt
    10741 atgacgggtc tcgtgacggt gtcgctgatc ggcatgacct gcgcgtacct gagcttgccg
    10801 ttcgaagccg cgttcatttt gttcctcgcc gtcgacccgc tgtgcgacat gctgcgcacg
    10861 ctgattctcg tgatcggaaa cacggcggca gtgacggtga tctgcccgcg tcccctcaaa
    10921 atctaagcct gggtgaatcg tatggcactg attggcattc tgggcggcat gggtccgctt
    10981 gcaacagtcg acttcttcga gcgagtcatc gaactgacca gttcggcggg cgcgtcctgc
    11041 gaccaggagc atttgccgct gctgctcgcg aacctgccgc acattccgga tcgctcgaaa
    11101 gcgatgctga aaaccggcga gaatccgctg cccgcgctgc tcgacggtat cgacatgctg
    11161 aaccgcaacg gcgtggagct gattgccatt ccgtgcaact ccgcgcattt ctggttcgac
    11221 gccatgcgcg agcatagccg cgcgccgatc ctgcacatcg ccgaggcatg tgttgcggcg
    11281 attccgcccg gcacgcgcgc ggcggccgtg ctggcgacgg gcggcacgtt gttgtcgggc
    11341 ctgtaccagc ggattctgga gcgtcacggg atcgagccgg tggtgccgaa cgagaccacg
    11401 caaaagcata tcgacgcatg cattcaggcc gtgaaagccg gctcgcttga cctcgcggcg
    11461 ttcgaactgc aagcggcact cgcggagttc gccgcacgcg gcgccgaggc ggccgtcatg
    11521 gggtgcacgg agattccgct ggccgcgcgc cgtatcgcgt cgccgtcgat gcagttgatc
    11581 gacagcacgc tggaactcgc tcgcgcgacg gtctcttatg ggctcgcgcg cggctggaac
    11641 gcccgtcaat gaaactgctg aagcgcctct cgaacagtac gctgatactg ctgctgtgct
    11701 ttgcgctcgg cggcctggcg ggcacgttcg cggcgcccat cggccagttt gctttcttca
    11761 tcgggcagat ttatctggcg ctggtgagca tggcggcggt gccgctgctg gtggtcgcca
    11821 tgtttttcgg gctgcgccaa ttgatgctgt tgtctcaccc gggcttgcgg atcagcacga
    11881 tgctgacgct ctcgcttgga ctcgtgttcg ccgccgcgct cgtcggcatg ctcacgggca
    11941 tggtggtcgc cccgggtctc catttgtccg acgctgcgca ccggcagttg ggccaactcg
    12001 tgctgcaaag cgccagcgac gcggaagaca tgcgcatgaa gctgttcggc accgacagtg
    12061 caggcgcggc gtcaagtggg ctcgcgctgc gcgacgtggt acccgacaac ttttaccgcg
    12121 tgctcgcggg cggtcacgcg ctcggcattc tgaccggcac gattctgttc ggtatggcat
    12181 tcgcggcgct ctctggcgag caaacgcgcg tgctgcatgg cgtattcgag agcatctatc
    12241 gcagcctcga aacgttgatc gaatatgcga acctgctgct gccggtgctc gctttcggca
    12301 ccgccgcgca tcttgtcgag cagacggagc acgagacgct cgacgcgatg agcggctttc
    12361 tcgtcagttt tctcgtctgc acggcggtgc tgtccacggt gattgtcgtg ctgatcgcgg
    12421 cacgtgcgcg cgcatcgctg gtgcgcgtgg ccgccgcgtt gcgtgcgccg atgctggtcg
    12481 gcctcacgtc cggcaacgcg agcgccacga tcccgcacac gatcgaagcg atgagcacac
    12541 gcctcggttt cagccgcggc gtcgcggaac tggtcgtgcc gttcggcagc gtttttgtgc
    12601 gcgcggggtc ggcactgtat ttcacgctcg ccaccgtttt catcgcaaat ctgtatgacc
    12661 ggccgctcgg cgtcggcgaa ctgctgctga cggccgcggc cgcgacggtc gccgcgttcg
    12721 cgtcggcggg ccgcaatggc gtggcgagca tttcatatgt gggcatcgtg ctgtcgctgc
    12781 tgcacttgcc ggtcgaagca gcgggcgtgc tgctgatcgc ggtggatctg ctctgcgacg
    12841 gtccgcgcaa tctgctgagc ctgctgtcgg tctgcacggt ggtcgccctg gtgtcggcgg
    12901 gattgcccgc tgagcgtgcg gcggcacctg agcacggcgt gcggcccgaa gccgcggcgg
    12961 tgctgcgctt tacgtttacg cgcgggcaac tcgtgctcgc gacgggctgt ctggtggcgg
    13021 cggcgtcgct gattgttttg atgggtgtcg gggtaggagc gaaatgaaca aggtgcaagc
    13081 gattctgaac tctggccggc gcggctttac ctgggcgaca gcgctcgggc gcctgcgcgt
    13141 cgcgtgtttt gccctgtgcg tggcgagcct tgtgagcggt tgcgggtcgt ttctgagttc
    13201 gaaaggcgag aaggtgcgct gggatcacgt gacgctcgtc gccgacgacg acgtcaacaa
    13261 caacagcccg gtcgcggtcg atgtggtctt cgtcaccgac gatgcgatgc tcgcgcgcat
    13321 tgccgagttg cccgcgtcaa agtggttcgc gagccgcgcc gatctggcga acacgttccc
    13381 gaagtcgctc cagtacaaaa gctgggagct ggtgccgggg cagcggatcg aggtgagcgg
    13441 cgattcgttc ggccgcccgc gtgtcgaagc ggcgttcctg ttcgccaatt acacggagcc
    13501 gggcgcgcat cgcatgcgga tcgaaaagct cagcggcaat ctcgccgtgc agctaaacac
    13561 ttatacgttc accgtctcgg cggtgaaatg agcgtagtcg gacaagtcat gaacacactg
    13621 aaaaccacac cggcgcatgt gtccggctcc gccgcgccac atgcggcgcc tgaccggagc
    13681 cacggccatg tttgaacaga tgacgccggt tgtcgatcgt attgaatggc atgaagggct
    13741 gctgctgtcg ccgcagcatt tccagcagat gagcgcgcgc gtcgattcgc tggtcgcgtg
    13801 gcagacgctg gccgccgcgc cgttcagctg gggcgtgcgc cgcctcgtgc tggacgccgg
    13861 tctgctgccc gcgggcacgc tgcgcgtgct ggcgctggag gcgatcatgc ccgacggcac
    13921 gtcggtttcg tactccgcgg attcgagcca gcaaggcagg ctcgatctgt cgctcgaacc
    13981 gtatgccgag cagctcgcga aagggccgct tgatttctac ctgaccttgc cgattgccgc
    14041 gtcgatgcgc cgtgacggcg aatcgcgccg gttccgctcg acgctcggcg cgccagtcga
    14101 agatgaaatc tcggacgcgc cgcccgccga cgtgccgcgc ctcataccga atcttggctt
    14161 gacggcgggc gaactgccgg cggcgagctt cgtgtcgctg cgcctcggca gcatctatcg
    14221 cgacaatgaa gtcgtccgac ttgccgagcg cctgccgccg ctaatcgaaa tttcgcgcga
    14281 tacgccgctg tggaccaacg tcgcgacgtt gctgggtcaa ctgcgcggca aggcggcgtt
    14341 cgttgcgcgt cagaccgcca atccgtcgtc caaggtcgac gaccgcctga cgcaactcga
    14401 actgaaagac cggctacgca gcctgctcgc cgcgttgccg ctcgtcgaag ccgtgctgcg
    14461 cacgccgcat ctgcatccgc tcgcgctgta tcacgcattg gccgcgctga gcggttcgct
    14521 cgcgatgctg gtgccgggcg gcttgccgcc ggtgccgccg gactacgacc atggcgatcc
    14581 gctggcggtc ttcacgccga tgctgcgcac cttgcgcgac gcagtgtccg aagtcgcgga
    14641 agggtatcgc gagcacaagt tcgaattccg tcatggtgca ttcgaaattg tgctgaacgc
    14701 ggaatggatc ggcgagcgga tcgtggtcgg tctacgcggc cagtccgacc gggatctgct
    14761 cgcgtggatg tccggcgcgg tgatcggctc gcagtccatt tacgcgatgc tgcgtactcg
    14821 ccgcgtgctc ggcgcaacgc gccgcgtcgt cgattacgcg gaagagttgg gcatccgctc
    14881 gggctccggc tatctgctgt tcgaaattca ggctgacccg tcgttgatcg tcacgagcga
    14941 agcgctcgtg atcagcaatc ccaacgaagg cgccaccgcg cagcggccgc aggaaatgct
    15001 gctgttcgtc aaaagctgag cgtcgggaaa gctcgaccca aaggtatccg tatggcacgc
    15061 aatcgaatcg aatccgaaga aaacgatctg gtgatgatgc agttccgcag ttttctcgac
    15121 gaactcgtga aagcgcagaa gcgcttttcc gacgcatcgg aaggcgacgc cgagcaggcc
    15181 gcgagcggcc tttcgcgcca cctgctaaat ctgctcgaag tccagacctt gcaggcgcat
    15241 cgcgacagca cgcgcttcga tctggagaac ctcgcggacg cacgctacct gaagaccgtg
    15301 ctcgccgacg aaattctgct gaacacgcag tgggccggcc gcggccactg gaccgcgtac
    15361 ctgctcgaat ccacgctgtt tcgcaccaat catgcgggcg accaggtgtt tcgcaggatc
    15421 gaacagttgc tgtccgaccg cgaaccgtcg cgccgggaca tcgcgcgaat ttgtctcttc
    15481 gcgctcgcga tgggctttca gggccgcttt cgcggcaccg acagcacggc gcgtctgcgc
    15541 agctatcgcg aagaactgta cgagtttgtc tatcaacgcc gcgcggatct gggcggacgc
    15601 gaccgcgtgc tcgcggatgc ggcatacggc aacacgattt cccatatcgc gccgcgcaag
    15661 ctgcctactg tgagtcgctg gaccgtgatc ggtctgctgg ccgttgccgc cttgctggcg
    15721 atttccgaaa tgttgtggct gtggcaatcg tggccggtgc gcgccgcgct gcatggcgac
    15781 gtggtcgcag gagtgaattc atgaagcagg cctgtgttgc gctattggcc ggagcgtcct
    15841 catgttgacg agcaatctct ttctgctgtc gctggcggtg ctgatcatcg tgatctgcgt
    15901 ggtgcttggc gccgtggtct atttcgcgct gcatggcgcg cacgcccagc cgcaagccga
    15961 gcgcaagatc gtgcgtttgc gcaccgattc gctgcgcagc gcattccgtc aggcggtcga
    16021 actgctggaa ggcaatatcg cgtctcgcgc ggagcgttac aacattccgt gggtgctggt
    16081 gctcaacgaa ggcgatgatc cgcgcccgct gccgatcgcg cagtcaggcg ttgcaagcgt
    16141 attgagcgcc gaggcggcta gccccgcggc gacgcaaggt atttcgtggc agttcttcga
    16201 ccgcggcatt gtggtcgaca tcaaggcggc ctatctcggc tcgccggatg aagagggcga
    16261 cgagaagccg tgggatgaat tcctgagctt gtgccgcaac tatcgcccgc agcggccgtt
    16321 cgattcggtg gtgatcaccg tgcccgcgtc gatgctgctc gcggaaaaca ccgacgcgcg
    16381 gctcgaactg gagcgtcatg cgaagctcgc acaccggcgt ctgtggctcg cgcagaaccg
    16441 ctttgcgatg cgcttcgcgg tgtatgtggt ggtaaccggt tgcgaggcgc tctccggttt
    16501 cgccgagttt gcgcgcgcgc tgccggaacc gctgagggcc agcatgctcg gctggtcgtc
    16561 gccgtacgat ctgtctgtca cgtatcagtc ggattgggtc gataccgcgc tgggcacgat
    16621 tgaacggacg gtatcggatg cgagtgcgga gctgtttgcg ctcgacgcaa acggcctcga
    16681 cgcacgcgag tgcctgctgc tgccgtcgcg tatcgacgcg atacgcgcgc agttgcaact
    16741 atatgtggat gaactgctgc gcgcgagcgc gtatcacgag ccgtttttct tccgcggcat
    16801 ctatctgact ggcgacagca gcgaactcgc gcagcgcagc gtggtcgcgc cggcgctggc
    16861 cgactatgcg gcggagggcg actacgacgc ctacggacgc ggacctgaac ccctgctcgc
    16921 gggctcggat tcggctgagg agggcgacgc aggcacggcc gtagtggtcg cgcaagagcg
    16981 cgcacgctcg gacagcgaat catacgatcc ggctcagact gtcaacgacc tgatgctgcg
    17041 ccccgcgttc ctgcgcgatc tgttcgacaa gaagattttc ctcgaatacg gtctgacgcg
    17101 gccttcgcgc tcgcaacatc tgacgcgtcc ggcgctgcat cgcggcttgc gctggggcgg
    17161 gatggcgctg ctcggcgtgt ggggcgtcgg cctggtggtg gcgacggtgc agttgaacca
    17221 tcgcaacggc gcgctggtca gcgcactcga cgaattgcgc aaggacagcc aggagcgcgc
    17281 cgccgccgaa cagggcggcg tgcaattgcc ggcggactgg tatcgccgca aggcgatcgc
    17341 gctgatcgcg ctcaacgacc gcttgcagac cggcacgccg tggacggtgt tcatgcccgg
    17401 ttcgtggagt gccgtcgatg atctgaacca gcgcgtgcac gagcgtttcg aacaggagtt
    17461 cggtgcgatt gcggtgacgg cgctggagcg cgagatgctc gcgcgcgtcg ggcagatgac
    17521 cggcgtggcg cgcgaccccg caacgggaca actggtggtc ggcgacgact gctcggcgcc
    17581 tcagaccatc ggcagcgatg cgccggttgc ggcgagcctc gcgcccgaag accagccggc
    17641 catgcgcgcg ctgcaacgct atgtcggtaa tgtggatcag ctcgatgccg cgcttcaggc
    17701 catggaccgt ttgcgccagc cttcgcagga caatgcggac gcgttgcggc ttgcggtgcg
    17761 ttatgcattc ggcgtggagc tgcagggcaa tgtcgcggcg agtctgccgt atttctaccg
    17821 caacgcgcgc cacggcggcg catatgcgtc ggacgccacc ggcggcatca atttgccggc
    17881 ggtacagcaa gcgctgcgtt gcacgttcga caaaggcgcg cagcaaatgg acgacgcgct
    17941 cttcacgtcg aatccgctgc tcgcgaccga gcgttcgatc acgacgcatc tgaatacgct
    18001 gtccgtcgcg gacgcgagcg ccgccgattt cacacaggtc acgaacaact atcgcgcgat
    18061 tgcggcgggc atcggcacgc aacaggattt gctcgcgacc ggcaagggcg catggatgcg
    18121 tcagacgcag ttcatgccgg gcgcaatcta tgaacgcacg tttgcacgcg cagcgcaaag
    18181 ccggctactc ggccccgatc tggtcgcgcg tatgcgcgag cgttccggca cgggctttca
    18241 ggcgttccgc agcgaactgg cgttgcgcgt gggcgcgccg gacagcggca tcgtatggac
    18301 cgaaaagggc gcacgctatg acgtggcgcc tgagcgtctc gcgctgcgtg acgggctgat
    18361 gaagctgctg aaccagccgt tcatggtcgc accgcgcggc ctgagcctgc cgacgctcgc
    18421 cgacggttcg gtggtcgcgt gggaccgcgt gcagctcgat caggcattgg cgctgagcga
    18481 cgtccgcaag cgtttcctcg ccgacggtct cgcgaatctg ccggaagcgg tgcagccttc
    18541 gatcgagcag gcggtggacg tgcagttcgc gcggctgatc ggcgatcagg tcgcggcggc
    18601 ggcaaccgtc acgcccgcgc agggcgagcc tgacggcggg aatttcgacg ccgcgcggtt
    18661 gcggctcacg aagattcgcg cagcgctggt ggatatgggc gcggcggcgc agatcgacga
    18721 tctcgatacg ctggtgtcgc gtgacgcgct cgcgcatctg cgtctcgtgg acgacgcgct
    18781 gaaccgctcc gagctgtatt cgacgcggcc cgcgcccgtc tccgtgtcgg gcaatgcggc
    18841 gtcgcacggc atgatgctgg ccgcgttcgg catcaacgac gtcggctcgc tcggggcgta
    18901 tctcgatcag caggccacgc gcgcgttggc gctcggacgg caggcgtccg cgtatcttgc
    18961 cgcgctcgga ccgggtgaag ccggttcgcc gctcgcgctg cgctggcagg caatcgtgcg
    19021 tgatctggat cgataccagt tgaagaaccc gaacagcagc ctgttcgcgc tggagcagtt
    19081 cgtgcagacc gttgcagccg atcctggcgc gggcgattgc atcacgcact tcccgccgcg
    19141 gcctagtgga ctcgcgacag gcgattactt cagcgcgctg catgcacgcc tttacgacac
    19201 gctgctcgcc cgctgtggtc agggctatac cgacgacctg cgtcagcaat ggaacggctt
    19261 ttccactgcg ttcaatcagg gtgtcgcggg gcatatgccg ttcggcgcgc cgggccgtgg
    19321 cgtgaacggt gacgccaaca cggcggattt ctccgaactg ggtcagacac tgcgtcaata
    19381 cgagcgcgtc tcgcacgtgt tccagcagtc caggagcggc gcgggggagc gggtgatcgg
    19441 cagcaggtcg ccggtgcgac agttcgtcga taacttcgac ctggtcgatg cgctgctcgc
    19501 tccgctttat ccggcgaccg acggtgctcc gtccggcctc gacgtcagcg tcgaattccg
    19561 cgcgaaccgc agcggcgaag tggccgccaa tcaggtgatc gactggacgc tgaagatcgg
    19621 cgaccagagc gtgtcgctgc acgacgcgcc gcatccgctg cattgggaat acggcatgcc
    19681 ggtttcgctg acgctgcgtc tcgcgaagga ttcgccgctt gcgtccgcga tcgacccgca
    19741 acagcgcgcg tattcgaccg atggacacac ggcgacatgg accttcggcg atccgtgggc
    19801 gctgattacg ttcgtgagcc gccagcgtgt cgccgatgcc ggcgcgcgtg gcgccaatac
    19861 gtcgcaactg ctgaaattcg agttcccgct cacggtggtg aacgtcgcgg acgaagccat
    19921 gctgccgaaa cagtcgcgcg gtcaggtgta cgtacgcctc gtactgagcc cgaccggcaa
    19981 gaaaaccgca ctgccttggc cgggcagttt cccgaaccgt gcacctgagt ggagcgcctt
    20041 atgaatgcgc ctcttttgct ggaagacgaa gctttgacgc agcccgacgg aatcggcgaa
    20101 tatctcgcgc cgcttcagga cggcaatggc gtgggcgttt cgctgcgcca cgatgccgta
    20161 ttccagaaga ttcgcgaagc ccgtcagcag gacgatccca cgttgccgat gcgcgagtgg
    20221 gagcgtccgc tgatcaaggc cgactggaac acggtcgctt cgttgagcgg cgaggcactg
    20281 cgtacgcgca gcaaggattt ccagcttgcg gcctggttat gcgaggcgtg gacccatcag
    20341 cgcggtgtcg aagggttttc ggatgggatg cgcttgctgc acgctctggt tgacggcttc
    20401 tgggagcacg cgtatccgca actcgaagac ggtgacagcg atgcacgcgt cgcgccgttc
    20461 gtctgggtca accagacgct ggcgatggta ctcaagctgc agattccgct gctcacactc
    20521 agcgtcgagc cgggttcgat caatttcgac gactggacgc gaattgtcgg ccgtgtgtcc
    20581 cacgacgagg aatcggaaga actggcgcgc gagttcctcg acggcgaggt cgacaagggc
    20641 tcgaaccttg ccgatctaat acgtctggaa aagcaactgg gcgtggctct cgatgtgacc
    20701 gcgacatttg aacgtggcct cgatatgcgt ctaggcaacg aagcgccgag cttttccaag
    20761 gtgctggagg cgctgatgga cttgaaacgg gtggcgaggt cgctgcgggg gagtcgtgca
    20821 atgccgatgg tcaacgaagc gcctgaggtt caggtgcaca acgaggttca aagcgtcgtc
    20881 acgaacgtag ctgacgacga aaacaatgcc tttgtcgtcg cgccgcattc gcccgctgcc
    20941 agcgacacga ccagcgtgat gacgccgttc cgtatcgcgg atcgcaccca cgcgtacgag
    21001 ctgctcgatg cggttgcgca atacctcgcg cgaaccgagc cgcacagccc gacgccttat
    21061 ctgttgaaac gtgcggttgc gtggggcggc atgccgttac ctgaactgat gcgtgaagta
    21121 gtgagccagg aaggcgatct gacacgctac ttcgcgatgc tggggctgca tgagtgacac
    21181 gaattcccgc gtttttaacc tgtggagcga gaacagccag tacaacgtct ctcgcacctc
    21241 gcgcagcaaa gttcgatgca ctatctgctt gaccgcgagg cgttgtgggg aaaggcgcca
    21301 ctcgcaaaaa cacttgccca agtttgcacg cacaggaaga caggtttcgc gtcccctcga
    21361 tttgctcagc ctcgagagaa cttgatgtag ttgcgtgaac cggtgcaatc agcaaccaga
    21421 tcaaaacgac cagaacaagg aaggatcaca tgcctgaagt caatggaata ccggagagca
    21481 aggagttcaa ggagcgtatc ggccgaacgg gtatttcctc tatcacgaag aaaaaggacg
    21541 gccagatgga tacgatcctg gcgtgtctgc agaacgcggg gtattggcgc gacgtgccga
    21601 ccccgaacgc catcgcgccg ctgcggctgg tacggaaaga gtgcattagc tggcttgtcg
    21661 ccaatccgcc gtcggtcacg cgcgcagccc ggccacatat cacccaactg ctcaacgcgg
    21721 tgaattcacg catcaatgca gtgatcgatc ggaatttcgt ggtgaacgac cagcgtacgc
    21781 cgcaacaact cattcagcgc ttaaaggaag gattcaatat aggcaccggt ttgtatgtcg
    21841 ccgaccgcaa cggtaccgtt acgcgaggcg gacgagtcgg caagcggctt gctgatgggt
    21901 atctggtgga gcggatgacc tcgacgcata tgccgaagat gcacttcgag ggcgcgttca
    21961 ccaagtacaa aaaggccaac accaagctca aattcgagac ctgggtccaa caggtattgc
    22021 ttccgcaaag cgaagacgac ccgatgggcg tgtatttcac cgcggacgac tcgcggcttg
    22081 gcaagtttaa tcaactcgcc tccgaggatc gggtccaata ttgcaacgag gacgagcgca
    22141 aggactatgt cattaccatt accgctggcg tggtaaagga tgccagtggt gatctctacc
    22201 atacgggcag taaggaaacc catttcagcg gccatggatg ggcgatctat gtggtcggca
    22261 tggagaacac gttttattcc aactcacacc tggtgaacaa attccaccac tcgagtttcc
    22321 tcgcgggcgc gcccgtccag ggtgcgggag agattgcggt taacaacggc aaagtggtcg
    22381 ctgtgacgaa taagaccggc cactactccg ctggacctgc cgagcttact cggacgctct
    22441 ttcttctgca ccgcggtggt gtcaatcttg acgaaatctt ggttaatgat ccgttcaggg
    22501 ctgcgaacgt atggatcacc ggccgtgatt gcctgcttgc cggtggagat ttcaacggaa
    22561 aggtgagctt gccgaagcct gcaacggttc ccgcctgaaa cagggtgaag cgcgcaagcg
    22621 caagaagccc gggtaccggg tgacgaatgc gcgcgcgtac aaccagagtc tgagaaagcg
    22681 cggcatgatc agcc
//
LOCUS       DATASH010000271         4011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5179109,
            whole genome shotgun sequence.
ACCESSION   DATASH010000271 DATASH010000000
VERSION     DATASH010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5179109"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="VHV99_07545"
     CDS             complement(<1..836)
                     /locus_tag="VHV99_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012400640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HEX3635328.1"
                     /translation="MKVAIVGAGLIGHTIAHMLRETGDYDVVAFDRDQHALDKLAAQG
                     IPTRRVDSADAAALRAAIQGFDTLVNALPYYLAVNVASAAKGAGVHYFDLTEDVRATH
                     AIRAIADDAEHAFMPQCGLAPGFIGIAAHELANRFTEIRDVKMRVGALPEFPTNALKY
                     NLTWSVDGLINEYCQPCEAIRDSRTQWVQPLEGLEHFSLDGTEYEAFNTSGGLGTLCE
                     TLSGRVESLDYKSVRYPGHRNLMQFLLEDLRFSSDRDTLKSIMRRSVPSTAQDVVLVF
                     ITV"
     gene            complement(1061..1519)
                     /locus_tag="VHV99_07550"
     CDS             complement(1061..1519)
                     /locus_tag="VHV99_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX3635329.1"
                     /translation="MRPPRLDQLDDLDRNLVALLQANARESVANLARQLDVARTTVIA
                     RIARLERSNVIGGYSVRLGQDVLDSSIQAYVGIIIAPKHGPAVQKRLGKMPEVQLLCA
                     VSGEFDYVAWLRADSPDRLNDLLDQIGGLEGVERTTTSIILARKIDRGMP"
     gene            complement(1787..2004)
                     /locus_tag="VHV99_07555"
                     /pseudo
     CDS             complement(1787..2004)
                     /locus_tag="VHV99_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070237.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            2189..2698
                     /locus_tag="VHV99_07560"
     CDS             2189..2698
                     /locus_tag="VHV99_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEX3635330.1"
                     /translation="MKKALVMLATAVAMSGAFAQTSAPAQASMGASASAPAGKPAHER
                     NVEDRIAYLHTQLKITSAQESQWNAFADVMRNNGQTMGQLFQQRKADTNLSALDDMKQ
                     YATIAQAHADGMKKLVDAFDPLYSSFSPEQKKLADVTFRQGGPEGGRRHHGKGASKKA
                     PANDATVKP"
     gene            complement(2899..>4011)
                     /locus_tag="VHV99_07565"
     CDS             complement(2899..>4011)
                     /locus_tag="VHV99_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635331.1"
                     /translation="GATPVPPSVKGNGHWVIADGVSLASDINNVKKQPNMGNITTVTA
                     GSALTVGDTLALINASGNHVLTLASKSLSLLGTAIGIAPSVGQLANDVKQLIQNPKDE
                     QSKWNVANSAFQLGAGLAATAASLVFPPAALVPLVFPNLAEIGHAEILRGKKNDLLAQ
                     GLNTEAAAVEDRYKIAALDATPIVNWFSSTYTPELRPAIESFEAAQGNVPGGKPAGEL
                     PVNTLHDQRVYDYYAQAITERAQRLAVAAKDYLKGIASGSDLSSVTMVSRCPQVFGWP
                     TNGQAMRVFDRAVAISYDRVHDTIRADLFGADKDGTYRLPTRDPSLPSGKGSKNLLFY
                     TNMLDPDAKRAKFDMPQYSADPGTFAIDVRKSADKA"
BASE COUNT          797 a         1192 c         1226 g          796 t
ORIGIN      
        1 acggtgatga acaccagcac gacgtcttgc gcggtcgagg gcaccgaacg gcgcatgatg
       61 ctcttgagcg tatcgcggtc gctggaaaaa cgcagatctt ccagcaggaa ctgcatcagg
      121 ttacggtgac ccggatagcg caccgacttg tagtcgagcg attccacccg gcccgacagc
      181 gtctcgcata gcgtgccgag gccgccggac gtattgaagg cttcgtattc ggtaccgtcg
      241 agcgagaagt gttcgaggcc ttcgagcggc tgcacccact gcgtgcggct gtcgcggatc
      301 gcttcgcacg gctggcagta ctcgttgatc agaccgtcga cgctccacgt caggttgtac
      361 ttcagcgcgt tggtcgggaa ctccgggagg gcgccgacgc gcatcttgac gtcgcggatc
      421 tcggtgaaac ggtttgccag ttcatgcgcg gcgatgccga tgaagcccgg cgccagaccg
      481 cactgcggca tgaaagcgtg ctcggcgtcg tcggcgatcg cgcggatcgc gtgggtggcg
      541 cgcacgtctt cggtcagatc gaagtaatgg acgcccgcgc ccttggcggc cgaagccacg
      601 ttcacggcca ggtaatacgg cagcgcgttg acgagcgtgt cgaagccctg aatggcggca
      661 cgcagcgcgg cggcatcagc ggaatcgacg cggcgggtcg ggatgccctg ggcggcgagc
      721 ttatcgagcg catgctgatc gcggtcgaac gcgacgacgt cgtagtcgcc ggtttcacgc
      781 aacatgtggg cgatggtgtg acctatcaac cctgcgccaa cgatggctac tttcatacgc
      841 ttctccttgt tctatgtacg ctgtctccag cttcgaaggt gcgctgcgcc aaggcgctgc
      901 gcccgtcggg ctgaaccctt gagagacagt ttagggagaa gcgaaaacag ttcatacgcg
      961 caaaatgctg cgctatgacc ataaccttcg acgttatgac gatgcggtta gtcgatatgt
     1021 cgaaaacata tcaaaacggc gtaaaaatca ggctgccgga tcacggcatg ccgcgatcga
     1081 tcttgcgcgc cagaatgatc gacgtggtgg ttcgctcgac cccttccagg ccgccgatct
     1141 ggtcgagcag atcgttgagg cggtcgggcg aatcggcgcg cagccacgca acataatcga
     1201 attcaccact caccgcgcat agcagttgca cctcgggcat cttgccgagc cgtttctgca
     1261 cggccggccc gtgcttcggt gcgatgatga tgccgacgta cgcctggatg ctcgaatcga
     1321 gcacgtcctg gccgagccgc acgctatagc cgccgatcac gttgctgcgc tcgagccgcg
     1381 cgatccgggc gatcacggtg gtgcgcgcca cgtccagttg acgcgcgaga ttggcgacgc
     1441 tctcacgggc gttggcctgg agcagcgcaa cgaggttgcg gtcgaggtcg tcgagttggt
     1501 cgaggcgcgg aggtctcatc gagggcggag gaagttagtc agaagtgtgt ggattatcgc
     1561 gcgtcttcga cctccggcgg cgaagtggct caaccatgaa gccgtgtgcg caacggccgc
     1621 gtaccggctg cgatgttcac gttctgacgc ggtctctagc aaccggattg tcacggctgg
     1681 ccgcggtcga ctatccaaag ctatggcata cgctgccata ccaaagcagc actgccactc
     1741 acactccaaa ccgctcaatc acgaaatcga tgaagctggc gtccgtcaga atgcgccgac
     1801 actgctcgta gtattgccga ccgatttcag tcaggctctg acagcgcgtg gtgcgcgtca
     1861 gaacccgcat cgcgagccgt gtttcgagtg agcggaatgc ttgcccacca gcgccgatga
     1921 aatgtcgaag cgctcggtgg ccgctgtcag actgcccgct tcgaccaccg cgacgaaaaa
     1981 ttccatgctg acgaatttgt ccaccggtat tgcctttcgt ttcggttgta agcgtttgtc
     2041 gcacaagagc cgcattgtag ggatatcgtg atacaaatag cgacgacggc gaagtgggca
     2101 gccggccgcc ttgcgtcgcc atgccgatgc gtgtgggccg ctcgccatgc tcgccttgtc
     2161 ctaattcgaa cagacatcag gagtttcgat gaaaaaagca ctcgtaatgt tggccaccgc
     2221 agtcgccatg agcggcgcgt tcgcacaaac ctccgcgccg gcgcaggcat caatgggcgc
     2281 ctcggcttcg gcccccgccg gaaaacccgc ccacgagcgc aatgtcgaag accgtatcgc
     2341 ctatctgcac acacaattga agatcacttc ggcacaggaa tcgcaatgga acgcgttcgc
     2401 cgatgtgatg cgcaacaatg gccagacgat ggggcagttg ttccagcagc gcaaggccga
     2461 cacgaatctg tctgcactcg acgacatgaa gcaatacgcg acgatcgcgc aagcgcatgc
     2521 ggatggcatg aagaaactgg tcgatgcgtt cgacccgctg tatagcagct tctcgccgga
     2581 gcaaaagaag ctggccgacg tgacgttccg tcagggcggc cctgaaggcg gccgtcgcca
     2641 tcacggcaag ggcgccagca agaaggcgcc cgcgaatgat gcgaccgtga agccgtaaga
     2701 ggctcactca cggtggattg cgtgcggcgc aggtgtttgc gccgcgcgcc tggttccttg
     2761 ccttcgaggc gcttcccggt gtcgatctgc ggtgcgtgcc cgccgcgttg gccatgcctt
     2821 gagttgatat tgatttgcta gctgtccctg tgcgggacgc ccccggccct caggccggga
     2881 ggatgtcaac agcgcccatc aggctttatc cgcgctcttg cgaacatcaa tcgcaaaagt
     2941 acccggatcg gcactgtatt gaggcatatc gaacttggcc cgtttggcat cggggtcgag
     3001 catattcgta tagaacagca ggtttttgct gcccttgccg gaaggcagcg atggatcccg
     3061 ggttggtagc ctgtatgtgc cgtccttgtc cgcaccgaag agatcggcgc gaatcgtatc
     3121 gtggacgcgg tcataggaaa tagccactgc ccgatcaaac acgcgcatgg cctgtccgtt
     3181 cgtcggccag ccaaaaacct gtggacaccg cgacaccatc gtgaccgaac tgaggtctga
     3241 accgctcgca atacccttga gatagtcctt ggcggcaacg gcaagccgct gggcacgttc
     3301 cgtgattgcc tgcgcatagt agtcgtagac acgttggtcg tgcagcgtgt tcacaggcaa
     3361 ttcacccgct ggcttacctc ctggcacatt tccttgcgcc gcctcgaagc tctcgatggc
     3421 gggacgtaac tccggcgtat aggtggaaga aaaccagttc acgatcggcg tcgcgtcaag
     3481 cgccgctatc ttgtagcgat cttcgactgc cgccgcctcg gtattcaggc cctgcgcgag
     3541 tagatcattc ttcttacccc tcaatatctc tgcgtgacca atctcagcaa gattcggaaa
     3601 gacgagaggc acaagtgcgg ccggcggaaa aaccagagag gctgcggttg cggccaaccc
     3661 tgcgcccagt tgaaaagcgc tgtttgcaac attccatttc gactgttcgt ccttcgggtt
     3721 ctggatcaac tgtttgacgt cattcgcgag ttgcccgacc gacggcgcaa taccaatcgc
     3781 cgtgcctagc agcgaaaggg acttcgatgc caacgtcagt acgtggtttc cactggcgtt
     3841 gataagtgca agggtatcgc caacggtcag cgcgctacca gcggtgactg tcgtgatgtt
     3901 ccccatattg ggttgcttct tgacattgtt aatatcggat gcgagggaga cgccgtccgc
     3961 aatcacccaa tggccgtttc ctttaacgct gggcggcaca ggagtcgctc c
//
LOCUS       DATASH010000272         7339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_234719,
            whole genome shotgun sequence.
ACCESSION   DATASH010000272 DATASH010000000
VERSION     DATASH010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7339
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234719"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..131)
                     /locus_tag="VHV99_07570"
     tRNA            complement(56..131)
                     /locus_tag="VHV99_07570"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(96..98),aa:Ala,seq:cgc)
     gene            complement(207..527)
                     /locus_tag="VHV99_07575"
     CDS             complement(207..527)
                     /locus_tag="VHV99_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015001430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX3635332.1"
                     /translation="MLTVCAWAGGFASSARAADALRGQVVASSNACMGCHAVDRKLVG
                     PSFLQIAARYKGDPQAPAKLARKVKDGGSGVWGMIPMPAHQSMSDADIRTVVDWVLAG
                     APSK"
     gene            complement(554..1348)
                     /locus_tag="VHV99_07580"
     CDS             complement(554..1348)
                     /locus_tag="VHV99_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX3635333.1"
                     /translation="MSVFRVKRTLVRTVLVVGFAAGLSATLQGCFLAVAGAAGGGALM
                     ATDRRTLGAQTEDRELQVKALSQISQNLPDNAHVNVAVFNRRVLLTGEVAGDVSKQRA
                     ESIVRGLNNVNTIVNELAIMPASSFSSRTNDTYLETRVKTALIAEKNISANNFKVVAE
                     RGSVYLMGLVTMDEGNRGADVASRVPGVVQVVKVFQYIQPQEAAAAAAAAATAPVAAP
                     QPDANAPTVGAIPDSSVSSRPLEQQSPAPVTNSNSVHPGNPKATTP"
     gene            complement(1375..1962)
                     /locus_tag="VHV99_07585"
     CDS             complement(1375..1962)
                     /locus_tag="VHV99_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HEX3635334.1"
                     /translation="MSVERIQQHFRDSAAVKLEALETLSMPIAAAIDTMFAALANGSR
                     ILACGNGGSAADAQHFAAELIGRFERERPGLPAIALTTDTSVLTAIANDYSFEQIYSK
                     QVWALGQPGDVLLAITTSGNSANVLAAVEAAHEREMIVVALSGKGGGRMQDVLSDTDI
                     HICVPSDRTARIQEVHLLTIHCLCDGIDAMLLGED"
     gene            complement(2190..2600)
                     /locus_tag="VHV99_07590"
     CDS             complement(2190..2600)
                     /locus_tag="VHV99_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEX3635335.1"
                     /translation="MAANPSASPHNFSRATGSKVVGAAFEARAMEFLQRQRLRFVARN
                     VLCRGGEIDLVMRERDGALVFVEVRARAQKHFGGAAASIGWQKKQRIVRAARHYLATR
                     SRHSHDEPACRFDVIAFEAGRLVWLRDAFRADEV"
     gene            2752..3630
                     /gene="rsmI"
                     /locus_tag="VHV99_07595"
     CDS             2752..3630
                     /gene="rsmI"
                     /locus_tag="VHV99_07595"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEX3635336.1"
                     /translation="MTPLSELAQGQQYPAAALYVVATPIGNIADVTLRALHVLGLVDR
                     IAAEDTRNTGQLLARYGISKPLVAVHQHNERAAALRLIEHLQAGERVAYVSDAGTPGI
                     SDPGAKLVDAVREAGFPVIPLPGASALATALSAAGDWVATFSFLGFLPPKAKARAAAL
                     QPLASPPHAMVFYEAPHRIVETVQALADAFGGERKLLIARELTKLHEALHCGTLAEGP
                     TWLAADPNRQRGEFVLVVEGAQPEAAGEHDHDALLAILLEELTVSSAAKVAATLTGAS
                     RNALYTRALALKKDAE"
     gene            complement(4094..4726)
                     /locus_tag="VHV99_07600"
     CDS             complement(4094..4726)
                     /locus_tag="VHV99_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEX3635337.1"
                     /translation="MRLDNLIARLMKTRLSRGLGTLFAFFVLAGCATPPGASNISASD
                     APLSTKNVQAGSFGPQAFGSAPASDAAAGSTPLADAKPITDDGSGISDFEQTGRASWY
                     GRGFHGRRTANGERYDMHAMTAAHRTLPLGSYVRVTNPATSRSVIVRINDRGPYARGR
                     VIDLSMAAASALDMRRAGTARVTIEGLSPQEARAELNETLASNSGPSIEK"
     gene            5335..5979
                     /locus_tag="VHV99_07605"
     CDS             5335..5979
                     /locus_tag="VHV99_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012399543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX3635338.1"
                     /translation="MKVTLIPVTPFQQNSSLLVCEATGRAAVVDPGGDLDVIQGEIAR
                     QNVTVEKVFLTHGHIDHCAGAKTLATHYGVPIEGPHPDESFWLDKLPDQSTRFGFPAA
                     EAFEPNRWLQDGETVKFGDETLEVYHCPGHTPGHVVFFSRAHRLALVGDVLFAGSIGR
                     TDFPRGNQADLVRAIREKLWPLGDDVTFVPGHGPTSTFGAERRTNPYVADGVEA"
     gene            5976..6605
                     /locus_tag="VHV99_07610"
     CDS             5976..6605
                     /locus_tag="VHV99_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HEX3635339.1"
                     /translation="MSSEIYVSTDVEADGPIPGPHSMLSFASAAYTEDKQLIATFSAN
                     LELLDGARPHPVQEAWWKTQPEAWEACRKDLRDPLAALTAYVEWVEALPGKPVFVAMP
                     AGFDFTYMFWYMMRFVGRCPFSWSALDIKTLAFALTGLPYRKNIKPRFPKHWFDEHPH
                     THVALDDAIEHGALFCNMLKDLRAVQAATLAAGKDGDGSGQNAAEEPAN"
     gene            6696..7154
                     /locus_tag="VHV99_07615"
     CDS             6696..7154
                     /locus_tag="VHV99_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008352298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX3635340.1"
                     /translation="MTLDTFSQKILRLLQLDARRSVQEISDQVGLSSTPCWRRIKDME
                     QSGVIQRYTALLDREKLGLHVCALAHIHLTRHTEGGVEQFEREIATCPEVTECYSTTG
                     ESDYILKIVAPDIKAYDSFLHERIFKIPAVAQVRTSVVLREIKFDTQLPL"
BASE COUNT         1406 a         2409 c         2194 g         1330 t
ORIGIN      
        1 acgctagaaa cacaaaaggg ttacaggcga atcagccgta accctttgat tttattggtg
       61 gaggagagga ggatcgaact cccgaccttc gcattgcgaa cgcgacgctc tcccagctga
      121 gctacccccc caacgtgcaa acaattctag cacaagcatt ttccgttttg ccaagccgcg
      181 aggcggcaaa aaccggcgtc aaaccatcac ttcgatggcg cgccggccag cacccaatcc
      241 accaccgtgc ggatgtccgc atcgctcatc gattgatgcg ccggcatcgg aatcatgccc
      301 cacacgccgg agccgccatc cttcaccttg cgcgccaatt ttgccggcgc ttgcggatcg
      361 cccttgtatc tggcggcaat ctgcaggaac gacggaccga ccagtttgcg gtccaccgca
      421 tgacacccca tgcaggcatt cgagctggca acgacctgac cgcgcagcgc atcggccgca
      481 cgcgcggaag atgcaaaccc gcccgcccac gcgcaaaccg tcaacatcgc agtaacgaaa
      541 acaacgcccc gcttcatggc gtcgtcgcct tcggattacc cggatgcacc gagttcgaat
      601 tggtcaccgg cgccggcgac tgctgttcga gcggacgcga actcaccgac gaatcgggaa
      661 tcgcaccgac ggtcggcgcg ttcgcgtccg gctgcggcgc ggcaaccgga gcggtcgccg
      721 ctgctgcggc agccgccgcc gcttcctgcg gctggatgta ctggaacacc ttcacgacct
      781 gcaccacgcc cggcacacgg ctcgcgacgt cggcgccacg attaccttcg tccatcgtga
      841 caaggcccat cagatacacc gagccacgct cggccacgac cttgaagttg ttcgccgaaa
      901 tgttcttctc ggcgatcaac gcggtcttca cgcgcgtctc gaggtaagtg tcattcgtgc
      961 gcgatgaaaa cgagctggcc ggcatgatcg ccagttcgtt gacgatcgtg ttgacgttgt
     1021 tcaagccacg cacgatgctc tccgcgcgct gcttcgacac gtcacccgcc acttcgccgg
     1081 tcagcagcac gcggcgattg aataccgcca cgttgacgtg cgcgttgtcg ggcaggttct
     1141 ggctgatctg cgacaacgcc ttaacctgca actcgcgatc ctcggtctgc gcgccaagcg
     1201 tgcgccggtc ggtcgccatc agcgcgccgc cacccgccgc gccggcaacg gcgagaaagc
     1261 agccctgcaa tgtcgcggac agtcccgccg cgaacccgac caccagcacg gttctcacca
     1321 gtgtcctctt gacgcggaat acgctcatca actagctctc cttcggaatc aatctcagtc
     1381 ttcgcccagc aacatggcat cgatgccgtc gcacagacaa tggatggtca gcaaatgcac
     1441 ttcctgaata cgcgcagtgc ggtcggacgg cacgcaaatg tggatatcgg tatcgctcag
     1501 tacgtcctgc atacggccgc cgcccttgcc gctcagcgcg accacgatca tttcacgctc
     1561 atgcgcggct tcgaccgcgg ccagcacatt ggcagagttg ccagacgtgg tgatggcgag
     1621 cagcacatcg cccggctgac caagggccca cacctgcttt gaatagatct gctcgaacga
     1681 gtagtcgttg gcgatggcgg tgagcacgga cgtgtcggtg gtcagcgcga tcgccggcaa
     1741 acccgggcgc tcacgctcga agcggccgat cagttcggcc gcgaaatgtt gcgcatcggc
     1801 cgccgagccg ccattgccac acgccagaat gcgactgccg ttggcaagcg cagcgaacat
     1861 cgtgtcgatc gcagcggcga tcggcatcga cagggtttcg agggcttcga gtttgactgc
     1921 cgcactgtcg cggaagtgtt gttgaatgcg ttcgactgac atcgagtctc tatttctacc
     1981 gcgagtgaac cgcagtgcgc ggccgtgttg tgaagacgta ttgcaaggtc gtgccgctcc
     2041 gctgaaccat gccggcaacg cagtgcgagc gcgcaaaata catcgcgaaa tgcgctgctt
     2101 ccttactgcg tcctatatgc cggcgtggtt cacgaggccc gcacgcgagc gcaagtttat
     2161 cgcactcacg ggcgtctccg cgccctgcgt cagacttcgt cggcgcgaaa tgcatcgcgc
     2221 agccacacga gccgacccgc ttcgaacgcg atcacgtcga aacggcacgc aggctcatca
     2281 tgcgaatgcc tgctgcgggt tgccagataa tgtcgcgcgg cccgcacgat gcgctgcttc
     2341 ttttgccacc cgatgctcgc tgccgcgccg ccaaaatgct tctgtgcacg cgctcgcact
     2401 tcgacgaaca ccagcgcgcc gtcacgctcg cgcatcacca gatcgatctc accgccccgg
     2461 cacagcacat tgcgcgcgac gaaacgcaaa cgctgccgct gcaaaaattc catcgcacgc
     2521 gcctcgaaag ccgcgccgac gactttggac ccggttgctc tcgaaaagtt gtgcgggctg
     2581 gcggatgggt tcgccgccac gctactcgcc gtactcgccg gcgcgcttgc ccgtgctgcc
     2641 gcagcagcac ctgtccgcgc actcaccgtc atcacgtggc tgccgcccgg ttcgcacgcc
     2701 gctgcgtggc acaatggcgg cctcgttctg tctgtctgcg tcttctgcct catgactcct
     2761 ctctccgaac tcgcgcaagg gcagcagtac cctgccgccg cgctgtatgt ggtggccacg
     2821 ccaatcggca acatcgccga cgtgacgctg cgcgcgttgc acgtgctcgg actggtggac
     2881 cggatcgccg ccgaagacac gcgcaacaca ggccaattgc tcgcgcgcta tggcatctcg
     2941 aagccgctcg tcgcggttca tcaacacaac gagcgggctg cggcgctgcg cctgatcgaa
     3001 catctgcaag cgggcgaacg cgtggcctac gtgtccgatg cgggcacgcc cggcatctcg
     3061 gaccccggcg cgaaactcgt cgacgcagtg cgcgaagccg gttttccagt cattccgctg
     3121 cccggtgcca gcgcgctcgc gaccgcgttg agcgcggccg gcgactgggt cgcgacgttc
     3181 tcgttcctcg gcttcctgcc gccgaaggca aaagcacgcg ccgcggcgtt gcagccgctc
     3241 gcgagccctc cgcacgccat ggtgttctac gaagcgccac accggatcgt cgaaacggtg
     3301 caggcattgg ccgacgcatt cggcggcgag cgcaaactgc taatcgcacg cgaattgacg
     3361 aagttacatg aagcgctgca ctgcggcacc ctggccgaag ggccaacgtg gctcgccgcc
     3421 gatccgaacc ggcaacgcgg tgaattcgtc ctggtggtgg aaggcgcgca gccggaggcc
     3481 gcaggcgaac acgatcacga cgcgttgctg gccatcctgc tggaagagtt gacggtgagc
     3541 agcgcggcaa aagttgcggc gacactcacc ggcgcttcgc gcaacgcgct gtatacgcgc
     3601 gctttggcgc tgaagaaaga cgcggaataa agcgggcgag cgccgtgcag gcgctcaggg
     3661 cagcagcggc gcatgcgctc cctgtacgcc atgccatggg tagagggatg tccgccaact
     3721 gattgggctc gcagcacaag ctacaagccc gtgatccgat catgcggaat cgcgacgcct
     3781 gcgctgcgcc gcacgcgcta gtccaacggc gcgcctcatc acaccgacgt ccgccggcgc
     3841 gatgatcgac acccgtgcct ccacctatcg cgcgttctgg ctacggctgc aaggaatgcg
     3901 ctactgcgtc tagccaccct ggatgagcaa aaaagggccg cgccatgaaa aacaccccaa
     3961 cggctggacg ggtgtccaac ttttggggtg cagttcacga tgcggccctt catctttcag
     4021 cgaacacggt gtgcgttgcc tccgtgcagc ccggcgtcgg ccacgcgcct caagcgcgcc
     4081 cgcagccgtt acatcacttc tctatgctcg gacctgagtt cgacgccagc gtttcgttaa
     4141 gctccgcgcg tgcttcctgc ggtgacaatc cttcgattgt cacgcgcgcg gtaccggcac
     4201 ggcgcatgtc gagcgcgctt gcagcggcca ttgagaggtc gatcacgcgg ccgcgcgcat
     4261 acgggccgcg gtcgttgatg cgcacgatca cagagcgcga cgtggccgga ttggtcacgc
     4321 gcacatacga accgagcggc aacgtacgat gcgccgcggt catcgcgtgc atgtcgtagc
     4381 gctcgccgtt ggcggtgcgg cggccgtgga agccacggcc gtaccacgag gcgcggccgg
     4441 tctgctcaaa gtccgaaata cccgaaccgt catcagtgat cggcttggcg tcggccagcg
     4501 gcgtgctccc ggcggctgcg tcggaggccg gcgcgctgcc aaaagcttgt gggccgaagg
     4561 agcctgcctg cacattcttc gtactgagtg gcgcgtcgct tgcggagatg ttgctagccc
     4621 ccggaggcgt ggcgcaaccg gccagcacaa aaaaggcaaa aagagtcccc agaccacggg
     4681 ataaccgagt tttcataagc cgtgcaatca aattgtcgag ccgcatcacg cgaaaagttg
     4741 agcgtgttgc ctgcgactcc ggcggcaatg ggcgaccacg cgccgcgcag acgagcgcag
     4801 tacgtcaaag cccggacgcg gctcacccag ccgctaccgc acggttaatt ttcacgtctg
     4861 gcgcaacctg tccccggcag cctgaccaga ccccacatgc cgccatcgaa cgtggcaaac
     4921 acgttcttgc gcgcggaact cagcgcacag gaacggcggc gtaggcccca cccagcgtca
     4981 cggcatttaa gtccgttgca ggcgcgcggc gcctggctgg gtaagacgtg tgcatcgaac
     5041 gcatcgatac ctgatggccg cccacaactg cgacagccca tacttgagtg gcgatccgcg
     5101 tgcccatttt tgatggggac gcatcgcctt gtggcatgca caattccttg tgcatggaac
     5161 gcattatacc taaaagaaaa ttttgggatg gcaagcgact gaaaaccttg atgaattttc
     5221 actcctgctg caaaaatggg cggccgaaag cccgctgcag cggggcctca gcgccttcaa
     5281 cgagcgtcga cgagacgccg gtacaatcaa cgtttttcca gcggcgcctc agccatgaaa
     5341 gtcactttga tccccgtcac gccgttccag caaaacagtt cgctgctcgt ttgcgaggcc
     5401 accggacgcg cggccgtcgt cgatccgggc ggcgacctgg atgtcatcca gggcgaaatc
     5461 gcgcgccaga acgtgacggt cgaaaaagtg ttcctcacgc atggccacat cgatcactgt
     5521 gccggcgcga agacgctcgc cacgcattac ggcgtgccga tcgaaggccc acatcccgac
     5581 gaaagcttct ggcttgacaa gctgccggat caaagcaccc gcttcggctt tcctgccgcc
     5641 gaggcatttg aaccgaaccg ctggctgcaa gacggcgaga ccgtgaagtt cggcgacgaa
     5701 acgctcgagg tctatcattg ccccggccac acgccggggc atgttgtgtt cttcagccgc
     5761 gcgcatcggc tggcgctggt aggcgatgtg ctgtttgccg gttcgatcgg gcgcacggat
     5821 tttccgcgcg gcaatcaggc cgacctggtg cgcgcgattc gcgaaaaact ctggccgctc
     5881 ggcgacgacg tcaccttcgt tcccggccac ggtcccacct cgacgttcgg cgccgagcgt
     5941 cgcaccaatc cgtatgtcgc tgatggggta gaagcatgag cagcgaaatc tatgtgagca
     6001 ccgatgtcga agcagacggt ccgatccccg gtccgcattc gatgctcagt ttcgcctccg
     6061 ccgcctacac ggaagacaag cagctaatcg ccaccttctc cgcgaacctc gaattgctcg
     6121 acggcgccag gccgcacccc gtgcaggaag cgtggtggaa gacgcagccg gaagcatggg
     6181 aagcctgccg caaggatttg cgggatccgc tggccgcgtt gactgcctac gtcgaatggg
     6241 tcgaggcatt gcccggcaag ccggtgttcg tggcgatgcc ggcgggtttc gacttcacct
     6301 atatgttctg gtacatgatg cggtttgtcg gccgatgccc gttttcgtgg tcggcactcg
     6361 atatcaagac gctggccttc gcactgaccg gcctgccgta ccgcaagaac atcaagccac
     6421 gttttccaaa gcactggttc gacgaacatc cgcatacgca tgtcgcgctg gacgacgcga
     6481 tcgaacatgg cgcgctgttc tgtaacatgc tcaaggacct gcgcgccgtt caggcagcca
     6541 cgctggcggc cggtaaagat ggggacggct caggacaaaa cgccgctgag gaaccggcaa
     6601 attaggtaat ctccctcgtc gaagccgttt tacattgcgt ttcgttttcg cgccctctac
     6661 catgcgttga taaggcgacg ccaacggagg cgcagttgac actcgatacg ttctctcaaa
     6721 aaatcctgcg cttgctgcag ctggacgcac gccggtccgt gcaagagatt tccgaccagg
     6781 tcggtctgtc gagcacccca tgctggcgcc gtatcaagga tatggagcaa tcgggggtaa
     6841 tccagcgcta cacggcgttg ctggatcgcg aaaagctggg gctgcatgtc tgcgcactcg
     6901 cgcatatcca cctcacgcgc cacaccgaag gcggagtcga acagttcgag cgggagatcg
     6961 ccacctgccc ggaggtcacc gagtgctaca gcacgaccgg cgaatctgat tacatcctca
     7021 agatcgtcgc accggacatc aaggcgtacg acagctttct gcacgaacgc atcttcaaga
     7081 ttcccgccgt cgcacaggtg cgcacgagcg tggtactgcg cgaaatcaag ttcgatacgc
     7141 aattgccgct gtgaggcggc ggggccgcgc gctttgagtc aggagtgcct tgctgccttg
     7201 ctgccttgat gccttgatgc cttggtgcct tggtgccttg gtgccttggt gccttggtgc
     7261 cttggtgcat tgatgcattg atgcattgat gcattgatgc attgatgcat tgatgcattg
     7321 atgccggcgt tttgaagcg
//
LOCUS       DATASH010000273         9559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6168601,
            whole genome shotgun sequence.
ACCESSION   DATASH010000273 DATASH010000000
VERSION     DATASH010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 9559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9559
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6168601"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..874
                     /gene="tsaB"
                     /locus_tag="VHV99_07620"
     CDS             56..874
                     /gene="tsaB"
                     /locus_tag="VHV99_07620"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEX3635341.1"
                     /translation="MTQTVLLALDTSTEFCSVALLSTDHVAGQAAGAARIWVRHEQTG
                     AVSSTRLLPAIRELFDEAGLKLADCHAIAFGSGPGSFTGLRTATGVAQGLAFGLNLPV
                     VPISTLLACAESARLRDPSATRVLAALDARMDEIYWADYAWDVALGEWRTHRPASLDA
                     PEGLVLPDVPFTLAGNAAAAFGARLPAVAAARAVDSEALPHAVPLAHAALRAFRAGRT
                     VPADQAAPEYIRDKVAQTTAERVADKAAKAEQAAVAAAIAARLAHDAQQGEGRQ"
     gene            871..1365
                     /gene="rimI"
                     /locus_tag="VHV99_07625"
     CDS             871..1365
                     /gene="rimI"
                     /locus_tag="VHV99_07625"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018417621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEX3635342.1"
                     /translation="MSGVLLADRYMSPMTEGDLDEVAAIEKIAYEFPWSRGNFGDSLR
                     NGYFGICLRHVTGTLIGYCVLMPVVDEMHLLNLCVTPAAQGAGAGLALLREAVRITRA
                     EKLEGLLLEVRPSNHRAIRLYERFGFASIGRRKNYYPARHRSREDAIVMRFSFATEGA
                     DGAA"
     gene            1352..2578
                     /locus_tag="VHV99_07630"
     CDS             1352..2578
                     /locus_tag="VHV99_07630"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX3635343.1"
                     /translation="MALHESVLEEFGLAPLWVRRGMSAAPESAGETALEQGAVAAAAS
                     VARPDDPRAAARDASPAALARDGRQAASASASASTNDTSSPAMHTGEARHADAARMQG
                     ERHAQSRAEQPSASTETRDRSDREASARASDSSSSMQERERRGPPPAHEQTQGRIQAA
                     VDARRPVKPPSQQLQAPEPPAFDAPPDDDFAWFDDQPAHAPAEARRDTPEPSSIQTLD
                     WDALSEQVAACERCRLCEKRTNTVFGVGDRNADWMLIGEAPGENEDRLGEPFVGQAGK
                     LLDNMLRSLTLARDTNVYIANVIKCRPPGNRNPEPDEVARCEPYLQRQVALVRPKLII
                     ALGRFAAQSLLRTEASISSLRGRVHEYEGVPVIVTYHPAYLLRSLPDKAKAWADLCLA
                     RDTWLAAGGVPSQAAK"
     gene            2719..3720
                     /locus_tag="VHV99_07635"
     CDS             2719..3720
                     /locus_tag="VHV99_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013338858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HEX3635344.1"
                     /translation="MLDTLREPVVRDLAWLLLSADLLRAQPPVGMLASPFDTSQEAEA
                     TVDWLRALDADPAELQRELTATRITRLGRYAERLLGWFLEHGPAARLVAASVPLRRAG
                     VTLGECDFLVKTQQGARQHWELAVKCYLHAGEGQVRSITQLDSHSVTQLSTPSTTHPA
                     ARLADYVGPNLKDRFDLKLAHVLNHQLPLSARDEFASVGYAGPWTPQMYIKGWLFYRV
                     GEAPAEPAELDPSHGRGWWVTRSEWPDFAAAHAQTWRLLPRLEWLAPRRHERREAEAS
                     GIAFADAHTLAEQISNQHGPTMVAAFADDGVGNLIERSRGFIVPDDWPGRAQAYAQQ"
     gene            complement(3742..4548)
                     /gene="thiD"
                     /locus_tag="VHV99_07640"
     CDS             complement(3742..4548)
                     /gene="thiD"
                     /locus_tag="VHV99_07640"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEX3635345.1"
                     /translation="MTQPIPNVLTIAGSDSGGGAGIQADLKAFSALGAYGASVITALT
                     AQNTRGVTAIHAPEPAFITAQLDAVFDDIRIDAVKIGMLANAQIARAVADALRRHKPK
                     HVVLDTVMISKSNHALLLPDAVAAVRDELLPLADLLTPNLPEAAALLGVPAATDEAGM
                     VEQGEALRALGARAVLMKGGHLSAADSPDWLVEDSGTLRLFGPRVPVKNTHGTGCTLS
                     AAIAALIPQRADLASAVADAKLYLTGALQASDRLDVGSGVGPVHHFYRWW"
     gene            complement(4605..5912)
                     /gene="lplT"
                     /locus_tag="VHV99_07645"
     CDS             complement(4605..5912)
                     /gene="lplT"
                     /locus_tag="VHV99_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011885164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HEX3635346.1"
                     /translation="MKKGFYTIMAAQFFSSLADNALLIAAIALLKDLHAPNWMTPLLK
                     LFFVLSYVVLAAFVGAFADSRPKGRVMFVTNTIKVVGCITMLIGAHPLIAYGIVGFGA
                     AAYSPAKYGILTELLPPDRLVAANGWIEGTTVGSIILGTVLGGALISPHIAAPILRQH
                     IPTVNTPAEAAMLVIMAIYVIAALFNLRIPDTGARYPKQERGPIKLVTDFADCFLVLW
                     RDKLGQISLAVTTLFWGAGATLQFIVLKWAEVSLNMSLSEAAILQAVVAVGVAAGAIF
                     AASRVPLKKSLTVLPVGIMMGIAVMLMAFYTRDLFPTHWGLYFGRVHVPGYLIVAYIF
                     LMIVGGLSGFFVVPMNALLQHRGHVLLSAGHSIAVQNFNENLSVLVMLCLYAVLVWLD
                     VPVTAVIVLFGTFVCVMMWFVMRRHQANQRAFDSVALIGEVKH"
     gene            6131..7201
                     /gene="alr"
                     /locus_tag="VHV99_07650"
     CDS             6131..7201
                     /gene="alr"
                     /locus_tag="VHV99_07650"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017331722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX3635347.1"
                     /translation="MPRPLSATIHTAALANNLAVARRYAPKSKVWAVVKANAYGHGLA
                     RAFPGLRATDGFGLLDLEEAVKLRELGWAGPILLLEGFFRSTDIDVIDRYSLTTALHS
                     DEQLRMLEMARLSKPVNIQLKMNTGMNRLGYTVEKFRSAWERARACQGVGQITLMTHF
                     SDADSDRGVAHQMEAFERGAQGIAGARSLSNSAATLWHPAAHFDWVRPGIILYGASPS
                     GVTAAIEGTGLQPAMTLTSELIAVQTLAEGHTVGYGSMYKARGPMRIGVVACGYADGY
                     PRVAPEGTPVIVDGVRTRIVGRVSMDMLTVDLTPVPTANVGSRAELWGASLPIDDVAQ
                     ACGTIGYELMCAVAPRVPVRAE"
     gene            7353..8729
                     /gene="radA"
                     /locus_tag="VHV99_07655"
     CDS             7353..8729
                     /gene="radA"
                     /locus_tag="VHV99_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006026043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEX3635348.1"
                     /translation="MAKQKTLYICSECGGQSPKWAGQCPSCNAWNTLVESVAEAPSTH
                     RFQSLAKSAPVRRLADIEASDVPRFSTGVSEFDRVLGGGLVPGGVVLIGGDPGIGKST
                     LLLQSLAEIAADKRALYISGEESAAQIALRAQRLSLLEPGSKASELQLLAEIQLEKIQ
                     ATIAEQRPDVAVIDSIQTVYSDALTSAPGSVAQVRECAAQLTRIAKQSGTTIIMVGHV
                     TKEGALAGPRVLEHIVDTVLYFEGDTHSSYRLVRAIKNRFGAVNELGVFAMTERGLRG
                     VANPSALFLSQHEQSVPGSCVLVTQEGTRPLLVEVQALVDAANAPNPRRLAVGLEQNR
                     LAMLLAVLHRHAGIACFDQDVFLNAVGGVKITEPAADLAVLLAIHSSMRNKPLPKGLV
                     VFGEVGLAGEIRPSPRGQDRLKEAAKLGFSIAVIPKANAPKQPTEGLQVVAVERIEQA
                     IDRVRTLE"
     gene            8872..9150
                     /locus_tag="VHV99_07660"
     CDS             8872..9150
                     /locus_tag="VHV99_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2866 domain-containing protein"
                     /protein_id="HEX3635349.1"
                     /translation="MKQSYETAAERPMQVRGCRVSEPIRQPWGGACRIVEWIDTAGQI
                     SRRVVAEHVTAAEVRATIDRHVEGRKHFLYDDEQTPRQVLPRQTAVRR"
     gene            complement(9328..>9559)
                     /locus_tag="VHV99_07665"
     CDS             complement(9328..>9559)
                     /locus_tag="VHV99_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX3635350.1"
                     /translation="GKRPLEEWVLLLESLDCCVSPVLTPAEAAQHPLFNREAYAAMNE
                     DQAENDAGEDGPPDRSSATEARLRSGKRQAKS"
BASE COUNT         1735 a         3065 c         3127 g         1632 t
ORIGIN      
        1 cgaagccttc cccaaaacca tatccactcg cgtggtgccg attacaatac ggtccatgac
       61 tcaaactgtg cttcttgccc tcgatacctc gaccgaattc tgctcggtag cgctcctgtc
      121 cactgatcac gtcgcgggcc aggccgccgg cgcagcccgc atctgggtcc gccacgagca
      181 aaccggcgcg gtctccagca cgcgcctgct ccccgcgatt cgcgaacttt tcgacgaagc
      241 cggcctgaaa ctggccgact gccacgccat cgccttcggc tccggtcctg gttcattcac
      301 gggtcttcgc acggcgactg gcgtcgcgca gggcctcgcg ttcggcctga acctgccggt
      361 cgtgccgatc agcacactgc tggcctgcgc ggaaagcgca cggctgcgcg atccatcagc
      421 gacccgcgtg ctcgcggcgc tcgacgcccg gatggacgaa atctattggg ccgactacgc
      481 atgggacgta gcccttggcg aatggcgcac gcatcgtccg gcttcgctcg acgcgccaga
      541 aggcctcgtt ttacccgacg tccccttcac cctggcaggc aatgctgctg cggccttcgg
      601 tgcgcggctg cccgccgtcg cggcggcgcg agccgtcgac agtgaagcac tgccgcacgc
      661 cgtgccgctc gcgcacgcgg cgctgcgtgc gtttcgcgcc ggccgtactg tgcccgccga
      721 tcaggccgcg ccggaataca ttcgcgacaa ggtcgcccag accaccgccg agcgcgtcgc
      781 cgacaaagcg gccaaggccg agcaagccgc ggtcgctgcc gcaatcgccg ccagactcgc
      841 gcatgacgcg caacaaggcg agggccggca atgagtggcg tgttactggc ggaccgctac
      901 atgtcgccga tgacagaagg cgatctggac gaagtcgccg ccatcgaaaa aatcgcctac
      961 gaattcccct ggagccgtgg caatttcggc gactcgctgc gcaacggcta tttcggcatc
     1021 tgtctgcgtc acgtaacggg cacgctgatc ggttattgcg tgctgatgcc ggtggtcgat
     1081 gaaatgcatc tgctcaattt gtgcgtgacg cccgccgcgc aaggcgcagg tgccgggctc
     1141 gcgctgctgc gcgaagcggt gcgtattacc cgcgccgaaa agctcgaggg cctgctgctc
     1201 gaagtgcggc cgtcgaatca tcgggctatc cggctttacg agcgcttcgg ttttgcgtcg
     1261 attggccggc gcaaaaatta ttatccggcg cggcatcgca gccgggagga cgccatcgtg
     1321 atgcgtttct cgtttgccac ggagggcgca gatggcgctg catgaatcgg tactggaaga
     1381 gttcgggctc gcgccgctgt gggtgcgtcg cgggatgtcg gcggcgccgg agtccgcggg
     1441 cgaaaccgcg ctcgagcagg gtgctgttgc ggccgcggct tctgtagcgc gtcctgacga
     1501 tccgcgtgcg gcggcgcgtg acgcgtcgcc ggctgcgctt gcgcgggatg gtcggcaggc
     1561 cgcgtccgcg tctgcgtccg cttcgacgaa cgacacatcg tcgcccgcga tgcacacggg
     1621 cgaagcgcgg cacgctgacg ctgccaggat gcagggcgag cgtcacgcgc agagccgggc
     1681 agagcagccg tctgcgtcaa cggagacacg tgatcgttct gatcgcgagg cttcggctcg
     1741 tgcaagcgat tcgtcgagtt ccatgcagga aagggagcgt cgtggaccgc cgccagcgca
     1801 cgaacagacg caagggcgca ttcaggcggc agtcgatgcg cggcgaccgg taaagccgcc
     1861 atcgcagcag ttgcaagcac ctgaaccacc ggcgttcgat gctccgcccg acgacgattt
     1921 cgcatggttc gacgatcagc cggcccacgc gcccgctgaa gcgcgccgcg acacacccga
     1981 gccgtcctcg atccagacgc tcgactggga cgcgttgagc gagcaggtcg ccgcgtgcga
     2041 gcgctgccgt ctgtgcgaga agcgcacgaa cacggtattc ggcgtcggcg atcgcaacgc
     2101 cgactggatg ctgatcggcg aagcgcctgg cgagaacgaa gaccgcctgg gcgagccgtt
     2161 cgtcggccag gctggcaagc tgctcgacaa tatgctgcgt tcgctgacgc tcgcgcgcga
     2221 caccaacgtg tatatcgcca acgtgatcaa gtgccgcccg ccaggcaacc gcaatcccga
     2281 gccggatgaa gtcgcgcgtt gcgagcctta tctgcagcgc caggtcgcgc tagtcaggcc
     2341 gaagctgatc attgcactgg gccgcttcgc ggcgcagagc ctgctcagga ccgaggcgag
     2401 catttcatcg ctgcgcggcc gcgtgcatga gtacgaaggg gtgccggtca tcgtcaccta
     2461 tcacccggcc tatctgctgc gcagcctgcc ggacaaagcg aaggcctggg cagacctgtg
     2521 cctcgcgcgc gatacgtggc tcgcggcggg cggcgtgccg tcccaagcgg ccaagtgacc
     2581 ggcgcgaccg ggccgacggc gcgcctcgcc gccgcgcgcg gcacatccgg tacatctggc
     2641 acacccggta cacccggtac acccggtaca cccggcacac ccggcacacc cggcacatcg
     2701 gcagcgctcc tcgaggcgct gctcgacaca ttgcgtgaac cggtcgtgcg cgatctcgcg
     2761 tggttgctgc tgagcgccga tttgttgcgc gcacaaccac cggtaggcat gctagccagt
     2821 cctttcgaca cgtcacagga agctgaagca accgtcgact ggttacgcgc gctggatgcc
     2881 gatcccgccg agttgcagcg cgaacttaca gcaacgcgta tcacgcgtct tggccgctac
     2941 gccgaacggc tgctcggatg gttcctggaa catggaccgg cggcgcgtct ggtcgctgcc
     3001 agtgtgccgt tgcggcgtgc gggcgtcacg ctcggtgagt gcgatttcct ggtgaaaacg
     3061 cagcagggtg cgcggcagca ttgggaactt gcggtgaagt gctatctgca tgccggcgag
     3121 ggccaggtgc gatcgatcac gcagttggac tcgcactcgg tcacgcagtt gagcacacca
     3181 tcgaccacgc acccggcggc gcgcttggcc gactacgtcg gcccgaatct gaaagaccgc
     3241 ttcgatctga agcttgcgca cgtgctgaat catcaactgc cgttgagcgc gcgcgacgag
     3301 ttcgcgtcgg tcggctacgc agggccgtgg acgccgcaga tgtatatcaa aggctggttg
     3361 ttctatcgcg ttggcgaggc gccggccgag cctgccgagc tcgatccgtc gcacggacgc
     3421 ggctggtggg ttacgcgcag cgaatggccg gactttgccg ccgcgcatgc gcaaacgtgg
     3481 cgtctgttgc cgcggctgga atggcttgcg ccgaggcgtc atgagcggcg tgaagcagag
     3541 gcttcgggaa tcgcttttgc cgatgcgcac acgctcgctg agcaaatctc aaatcagcat
     3601 ggaccgacaa tggtcgcggc atttgccgat gacggcgtgg gcaacttgat agaacgctcg
     3661 cgcggcttca tcgtcccaga cgactggccc gggcgcgcgc aggcctacgc gcagcaatag
     3721 cgcaggcgca aggcgcctca gtcaccacca gcgataaaaa tgatgcaccg gcccgacgcc
     3781 gctgccgacg tcgagccgat cgctcgcctg caaagcgccg gtcagataca gcttggcgtc
     3841 ggcgaccgcg ctcgccagat cggcgcgctg cggaatcagt gccgcaatcg ccgccgacag
     3901 cgtgcacccc gtcccatgcg tattcttcac cggcacgcgc gggccgaaga gacgcagcgt
     3961 gccgctgtct tctacgagcc agtcgggact gtcggccgcg ctcagatggc cccccttcat
     4021 cagcaccgcc cgcgcgccaa gcgcacgcag cgcttcgcct tgttcgacca tgccggcttc
     4081 gtcggtggcg gctggcacgc ctaacaatgc cgcggcttcc ggcagattcg gcgtcaacag
     4141 atcggccagc ggcagcaact cgtcacgcac ggccgcgacc gcatccggca gcaacagcgc
     4201 gtgattgctc tttgaaatca tcaccgtgtc gagcacgacg tgcttcggtt tgtgccgccg
     4261 caatgcatcg gcgaccgcgc gcgcgatttg cgcattcgcg agcatgccga tcttcaccgc
     4321 atcaatacgg atgtcgtcga acaccgcgtc gagttgcgcg gtgatgaagg cgggctccgg
     4381 cgcatgaatc gccgtaacgc cgcgcgtgtt ctgagcggtg agcgcggtga tcacgctcgc
     4441 gccgtaagcg ccgagcgccg agaatgcctt cagatcggcc tgaatgccgg cgccgccgcc
     4501 ggaatccgaa ccggcgatcg tcagcacatt gggaatgggt tgagtcatga caaagcgagg
     4561 aaaggggaaa ggcgcgggca gtcagcgcag tgcctgccgc ggggtcagtg cttgacttcg
     4621 ccgatcagcg cgaccgagtc gaacgcgcgc tgatttgcct gatggcgccg catcacgaac
     4681 cacatcatca cgcagacaaa ggtgccgaac agcacgatca ccgccgtgac cggcacgtcg
     4741 agccagacca gcacagcgta caggcacagc atcacgagta ccgagagatt ttcgttgaag
     4801 ttctgcaccg cgatcgagtg acccgctgac agcagcacgt ggccgcgatg ctgaagcagc
     4861 gcattcatcg gaacgacgaa aaagcccgac aggccaccga cgatcatcag gaagatatac
     4921 gcaacgatca gatagcccgg cacatgcaca cggccgaaat acaggcccca atgcgtgggg
     4981 aagaggtcgc gtgtgtagaa ggccatcaac atcacggcga tgcccatcat gatgccgacc
     5041 ggaagtaccg tcagcgactt cttcaacggc acgcgcgagg cggcgaaaat cgcgccggct
     5101 gccacgccga ctgccaccac cgcttgcagg atcgcggctt ccgaaagcga catgttcagc
     5161 gacacttccg cccacttcag cacaatgaat tgcagcgtcg cgccggcgcc ccaaaatagc
     5221 gtggtgacgg cgagcgaaat ctggccgagc ttgtcgcgcc acagcacgag aaaacagtcg
     5281 gcgaaatcgg tgaccagctt gatcgggccg cgttcctgtt tcggatagcg cgcgccggta
     5341 tcgggaatgc gcagattgaa gagcgccgcg atcacgtaga ttgccatgat caccagcatc
     5401 gccgcttcag ccggcgtgtt gaccgtgggg atgtgctgcc ggaggatcgg tgcggcaatg
     5461 tgcgggctga tgagtgcgcc gccgagcacg gtgccgagaa tgatcgagcc gaccgtggtg
     5521 ccttcgatcc agccgttcgc cgcgaccagc cggtcaggcg gcagcagttc ggtgagaatg
     5581 ccgtatttgg cgggcgagta ggccgccgcg ccgaaaccca cgatgccata ggcgatcaac
     5641 gggtgcgcgc cgatcagcat cgtgatgcag ccgacgacct tgatggtatt ggtgacgaac
     5701 atcacgcgac cctttggacg ggagtcggcg aaggcaccca cgaaggcggc cagaacgacg
     5761 tacgacagca caaagaacaa cttgagcagc ggcgtcatcc agttcggggc gtggaggtct
     5821 ttcagcagtg caatagcagc gatcagaagc gcattgtcgg ccagcgacga aaaaaactgc
     5881 gcggccatga tggtgtaaaa accttttttc atctgatgcg atgctgtcct cgctgcggtc
     5941 cgtccgcccc ggccgtgtgc aaagcgtccg ggcttatacc gttcgaaatg ggttgtgcgc
     6001 acggctttat atcatgaaaa tagctggatt cggactagca gaatccttga tcgcaccgcc
     6061 cagcgggccg ccgagcgctg aaaacgccct gtaagctcct gattcgcaag cgtctttacg
     6121 aaaattgccc atgccgcgcc ccctctcagc cacaattcat actgccgcac tcgccaataa
     6181 tctcgccgtc gcccgacgct acgcgcccaa atccaaggtg tgggccgtcg tcaaggccaa
     6241 cgcctacgga cacggcctcg cgcgcgcttt tcccggtttg cgcgcaaccg acggctttgg
     6301 gttgctggac ctggaagaag ccgtcaagtt gcgtgaattg ggctgggccg gcccgattct
     6361 tctgttggaa ggcttctttc ggtcgaccga tatcgacgtg atcgaccgct acagcctgac
     6421 cacggcgctg cactcggacg aacagttgcg gatgctcgaa atggcgcgcc tgtccaagcc
     6481 ggtcaatatc cagctgaaga tgaacaccgg catgaaccgc ctcggttaca cggtggaaaa
     6541 attccgctcc gcgtgggaac gcgcgcgggc ctgccagggt gtcggccaga tcaccttgat
     6601 gacccatttc tcggacgccg acagcgaccg cggcgtggct caccagatgg aagcgttcga
     6661 gcgcggcgca caaggcattg ccggcgcgcg cagcctgtcg aattcggcgg ccacgctgtg
     6721 gcatcccgcc gcgcacttcg actgggtacg cccgggcatc attctgtacg gcgcgtcgcc
     6781 ctcgggcgtg acggccgcga tcgaaggcac cggcctgcaa ccggccatga cgctcacctc
     6841 tgagctgatc gccgtgcaga cgctcgccga aggccacacg gtcggttatg gttcgatgta
     6901 caaggcgcgc gggccgatgc gcatcggcgt ggtggcctgc ggttacgcgg acggttatcc
     6961 gcgggtcgcg cccgaaggca cgccggtgat cgtcgacggc gtgcgtacgc ggatcgtggg
     7021 gcgggtgtcg atggacatgc tgaccgtcga cctgacgccg gtcccgacag ccaatgtcgg
     7081 ctcgcgcgcc gaactgtggg gcgcgtcgct gccgatcgac gatgtcgcgc aggcatgcgg
     7141 cacgattggc tacgagctga tgtgcgcagt ggcgccgcgt gtgccggtgc gtgcggagta
     7201 aggcacggcg ctgacaggcc gggtgagagc cgcgcatggc ggcctccatg gcggtaccgg
     7261 agagggtccc aaaggcggtt gcagtgagct cctccacgag gagcgctcag cgagcccgtc
     7321 aatccaaaga acagtaatac aggcaagggc gcgtggctaa acagaagacg ttgtacatct
     7381 gcagtgaatg cggcggccaa tcgccgaagt gggccggaca gtgcccgtca tgcaatgcgt
     7441 ggaacacgct ggtggagtcg gtcgcggagg cgccgtccac gcatcgcttc cagtcgctcg
     7501 cgaagagcgc gccggtgcgg cgtctcgccg atatcgaagc gtccgacgtg ccgcgcttct
     7561 caaccggcgt cagcgagttc gaccgtgtac tgggcggcgg cctcgtgccg ggcggcgtgg
     7621 tgctgatcgg cggcgatccg ggcatcggca aatcgacgct gctcttgcaa tcgctcgcgg
     7681 aaattgccgc ggacaaacgc gcgctctata tcagcggtga agaatcggcc gcgcagattg
     7741 cgttgcgcgc gcaacgactt tcgttgctgg aacccggctc gaaagccagc gagctgcagc
     7801 tgctggcgga aatccagctc gagaaaattc aggcgaccat cgccgagcag cgaccggacg
     7861 tcgcggtgat cgactcgatt caaaccgtct attccgatgc gctgacttca gcgcctggtt
     7921 cggtcgcgca agtgcgcgag tgcgcggcgc agttgacgcg catcgccaaa cagtctggca
     7981 ccacgatcat catggtcggc cacgtcacca aggaaggtgc gctggccggg ccgcgcgtgc
     8041 tcgagcacat cgtcgatacg gtgttgtact tcgagggcga cacccactcg tcgtaccgcc
     8101 tggtgcgcgc gatcaagaac cgttttggcg cggtcaacga acttggcgtg ttcgcgatga
     8161 ccgagcgcgg tctgcgcggt gttgccaatc cgtcggcgtt gtttctgtcg cagcacgagc
     8221 aatccgttcc ggggtcgtgc gtgctggtga cgcaggaagg cacgcggccg ctgctggtcg
     8281 aagtgcaggc gctggtcgat gcggccaacg cgccgaatcc gcggcgcctc gccgtggggc
     8341 tggaacagaa tcgtctggcg atgctgctgg cggtgctgca tcggcacgcc ggcatcgcct
     8401 gtttcgacca ggacgtgttc ctgaacgcgg tgggcggcgt gaagatcacc gaacccgcag
     8461 ccgacctggc cgtgctgctc gcgatccact cctcgatgcg caataagccg ctgcccaagg
     8521 ggctggtcgt attcggcgag gtggggcttg ccggcgaaat ccggccgtcg ccgcgcggtc
     8581 aggatcgcct caaggaagcg gccaaactgg gcttttcgat cgcggtcatt ccgaaggcca
     8641 atgcaccgaa acaaccgact gaaggcttac aagtcgttgc ggtcgaacgc atcgaacagg
     8701 ccatcgaccg ggtccgcacg cttgagtaga cggggctttg gcgctcgcgg gcgcgtttgc
     8761 tcccgtaatg tgccgtaaat atctccatat tggctgtaac ctacccgaca ttcctttttc
     8821 ctaagcttcg aaggtgttgc atcccttctg atgccgaaaa aggatcgcgc cttgaaacag
     8881 tcatatgaaa ccgcagccga gcggcccatg caggtgcgtg gctgccgcgt ctccgagcct
     8941 attcgccagc cttggggcgg tgcttgccga attgtcgaat ggatcgacac ggcagggcag
     9001 atttcccgcc gggtagtggc cgaacatgtt accgccgctg aagtgcgcgc gacgatcgac
     9061 cgtcacgtgg aaggccgcaa gcatttcctg tacgacgacg aacaaacccc gcgccaggtt
     9121 ttgccgcggc aaacggcggt gcggcgctga ggttttgggg gcggcgggtt aggcttcgcg
     9181 gcctgaatgg tgagttggtt gttgcaggcc acggacttgc aagatcgtca tgagccggga
     9241 aagcgagatc gcagacccgc cgatccgccg ccagcagacc ggccgaccgg cagaccagca
     9301 gactcgccga tccgccagac tgtctggcta cgacttagcc tggcgtttgc ctgatcttag
     9361 ccgcgcttca gtggcgctcg accggtctgg tgggccatct tcgccggcat cgttttcggc
     9421 ctggtcttcg ttcatcgcgg cgtatgcctc gcggttgaac agcgggtgct gcgcggcttc
     9481 cgcgggggtc agcaccggcg agacgcagca gtctagcgac tccagcagca gaacccattc
     9541 ttccagcggg cgcttgccg
//
LOCUS       DATASH010000274         4862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3003920,
            whole genome shotgun sequence.
ACCESSION   DATASH010000274 DATASH010000000
VERSION     DATASH010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3003920"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="VHV99_07670"
     CDS             <1..374
                     /locus_tag="VHV99_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4279 domain-containing protein"
                     /protein_id="HEX3635351.1"
                     /translation="HRKAAALFTGYSGVKPDTAIVKGEPFITPSGRTSRMPGRTSVWG
                     VRSKSAVRDDLLTPHLRYLVGYLGLPRADLRDLLNKQAARIRFFCYWANYTGDRVPDV
                     PDDIRALIESLGGVIEIDEYR"
     gene            complement(404..1651)
                     /locus_tag="VHV99_07675"
     CDS             complement(404..1651)
                     /locus_tag="VHV99_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3635352.1"
                     /translation="MLMENNLPAGAASPDDSASSSLRSWLAVGAVAIGAFAFVTTEFL
                     PVGLLPRVAADLDVLPGTAGLMVTVPGVIAAISAPGSMLVAGRMDRRRVFLLLTALLL
                     ASNLISALAPNFLLLLLGRALLGAALGGFWTLATAASGRLVQPKDSAKAMATILTGVT
                     CATVIGVPLGTFIASFASWRASFMATSVLVAIALVAQFVFVPSLPSNAALRMHDLVSL
                     MRRPHPRRSMLMVALVFGAHFSSYTYITPFLLRNANFTMSTITWLLLGFGIIGFVSNF
                     AVSSTVARNLKTSVAVMVSLLMFALLAMPLLQHSSIGVAALVLAWGVSFGALPLCFSV
                     WIQRATPDSPEAGSALFVSIIQVAIAVGSLIGGVVVDHVGISADFLFGSGLALLGLAA
                     LASFGRSEQGVVAEAIVGPVCSD"
     gene            complement(2072..2380)
                     /locus_tag="VHV99_07680"
     CDS             complement(2072..2380)
                     /locus_tag="VHV99_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635353.1"
                     /translation="MKLRLLMALASIALFLAPNPAGALDGPQDEAVPSRAFMQNANES
                     AQATTDMSFGETGRPTMPGAQAVQNVSYGGVATGTEEAGGRQERPCSSGPQCRIYFGR
                     "
     gene            2835..2963
                     /locus_tag="VHV99_07685"
     CDS             2835..2963
                     /locus_tag="VHV99_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635354.1"
                     /translation="MRQAIRDQCIVITVFSIAAIRLIQPYLPRGGALEYSLIVTDW"
     gene            3186..3512
                     /locus_tag="VHV99_07690"
     CDS             3186..3512
                     /locus_tag="VHV99_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HEX3635355.1"
                     /translation="MDERKRDSMIAYLRHRMEEFGIKPEDLAAVLAAEPKGQKAERYR
                     SATGDSWDGQGEMPQWLKQAISAGQSIDHFELTAAPAPAPQPRKQVDWLNDPFAGSPL
                     ARPNNR"
     gene            complement(3619..4119)
                     /locus_tag="VHV99_07695"
     CDS             complement(3619..4119)
                     /locus_tag="VHV99_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphatase family protein"
                     /protein_id="HEX3635356.1"
                     /translation="MSRKYKVLFLCRENSARSIMAEALLRELAGHRFDAFSAGPEPAA
                     RVHPIAIAQLRPGVSDLGVLSPKSWLEFTGEWAPRMDLVIAMDAGVAGDRAPLFPGEP
                     SMCLWTFADPLADEMPEPERVRLFEKVFWQIVRQLSTFIELPQYAVARDAAASVGSPA
                     CADEAQ"
     gene            4388..>4862
                     /locus_tag="VHV99_07700"
     CDS             4388..>4862
                     /locus_tag="VHV99_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007738483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3635357.1"
                     /translation="MNATTLNPAADRDKLESLAYAKVTWRLLPFLFLCYVAAYLDRVN
                     VGFAKLQMLNDLHFSDTVYGLGAGIFFIGYFFFEVPSNVLMHRFGARVWISRIMITWA
                     LISAATLFVTTPTQFYALRLLLGIAEAGFFPGIVLYLTYWYPSARRSRVNALFMVG"
BASE COUNT         1064 a         1491 c         1403 g          904 t
ORIGIN      
        1 accaccgcaa agccgcggcc ttgttcaccg gctattccgg cgttaaacct gacacggcga
       61 ttgtcaaagg cgagccgttc atcacaccat ctggccgaac cagccgcatg cccggtcgga
      121 ccagcgtgtg gggcgtgcgc agcaaatccg ctgttcggga cgacctcctc acaccccatt
      181 tgcggtatct cgtcgggtat cttgggctgc ctcgcgccga ccttcgcgac ttactgaaca
      241 agcaggccgc gcgaatccgc ttcttttgct actgggcgaa ttacaccggc gaccgtgtac
      301 ccgatgtacc cgacgatatc cgcgcattga tagagtctct cggcggtgtc atagagatcg
      361 acgaatatcg gtagcgggta ctttcgtgtt ttggttgaca gctttaatca ctacacaccg
      421 gacccacaat cgcctcggcc accaccccct gctcgctgcg cccgaaactc gcgagcgccg
      481 cgagccccag cagtgccaaa ccgctaccga aaagaaagtc ggcggaaatg ccgacatggt
      541 cgaccaccac gccgccaata agcgaaccca ccgcgatcgc cacctggatg atgctgacaa
      601 acagcgccga acccgcttcc ggcgaatccg gcgtagcgcg ttgaatccag acgctgaaac
      661 agagcggcag cgccccgaac gaaacacccc acgcgaggac gagcgccgcc acaccgatcg
      721 acgaatgctg caacagcggc atcgccagca gcgcgaacat gagcagggaa accatcacag
      781 ccacggacgt tttcaggtta cgtgccaccg tcgatgaaac ggcgaagttc gaaacgaagc
      841 caataatgcc gaagccgagc aacagccatg taatcgtcga catggtgaaa ttcgcgttgc
      901 gcaacaggaa cggcgtgatg tacgtgtacg aggagaaatg cgcgccgaat acgagcgcca
      961 ccatcagcat gcttctacgc ggatgcgggc ggcgcatcag tgacaccaga tcgtgcatcc
     1021 gcaatgcggc gtttgacggc aacgacggca cgaagacgaa ctgggccacg agcgcgattg
     1081 cgacaagtac gctggtggcc atgaacgaag cacgccacga cgcgaagctc gcgatgaaag
     1141 taccgagcgg cacaccgatc accgttgcgc aggtcacgcc ggtcaggatg gtcgccatgg
     1201 ctttggccga atctttcggc tgcacgaggc gccccgatgc cgccgtcgcc agggtccaga
     1261 atccgcccag cgccgcgccg agcaacgcgc gcccgagcag caggaggaga aagttgggcg
     1321 cgagggccga gatcagattc gacgccagca acaatgctgt gagcagtaga aacacacgcc
     1381 ggcgatccat ccgtccggcc accagcatcg aaccgggcgc ggagatggcg gcaatcacac
     1441 ccggcacagt caccatcaat cctgcggtgc cgggcagcac gtccagatcg gccgctacac
     1501 gcggcagcag tccaacgggt aaaaactccg tggtcacgaa tgcgaacgca ccgattgcca
     1561 cggctccgac agcgagccag gaacgcagag acgacgaggc gctatcgtcc ggcgaggcag
     1621 cgcccgccgg cagattattt tccatcaaca tagtattgtt ttgcgagtca ctaaacaacg
     1681 ggtgggattg gagcagtcgc accgagctaa tcaattcagg ggcagcccat cacgataaaa
     1741 aaagcaggcg atatttgcct gcttgtgatt tgcgcccgaa aaccggtggg tggagacctg
     1801 aacggaacac accataacat cgtattaaat cgcccgctgg ccactccgta aaatcgcagt
     1861 tgggcgtggg ggtatttcta tcagtatggc gggcgcggat gacacggtta tttcgcgcgt
     1921 cgttcagatt acgcggtgcg gggcggaccg catgattggt tcgcgcatcg cttcgatcat
     1981 gcggcgtacg tggagacagg gttggatcgc gcatcgaagt gcgagaggcg gtacgtgcta
     2041 ctccctcacc gcctcctgaa ctgccggtct gtcatcggcc aaaatagatc ctgcattgcg
     2101 gcccggaaga gcatggccgc tcctgccggc ctcccgcttc ctccgtgcca gtggcaacac
     2161 caccataaga gacgttctgc accgcttgcg cacccggcat cgtgggccgc ccggtttcac
     2221 caaacgacat gtcggtggtg gcctgtgccg attcgttcgc gttctgcatg aacgcccggc
     2281 tcggcacggc ttcgtcttgc ggaccatcca gcgcgccggc tgggttcggc gccagaaaga
     2341 gcgcgatgct tgccagcgcc atcaataaac gtagtttcat gtccagactc ctcgaatccg
     2401 gcgcagcagc gctcatcccc ccaaaaagaa tcgatccgcg aaaaacgcca cgtcaccaaa
     2461 tcgaactgac ggacgggcga atgcctatgc gggactgctg ccgcgagtca ccgaactaaa
     2521 cgagtaaata gttgccagac ctgaaaccga tgtcgggcgg ccacgcatgg cttgccggaa
     2581 ccggtcgcca ccaggctgga aacgcggatt gtcgctccgc tgtttcgtta taggtcggcc
     2641 gcccgggatt tcaatcccgg cgatgcggac tgggttagcg gcgtcggtgg gagccctatt
     2701 agggacattc aggggcattc gacaacaaaa tcagtggggc cagcgcatgc tttacccgaa
     2761 cagtgcgcta tgatgctgga ataacacaca caatcagccc cgcgcgaagc accggcgaga
     2821 gacggagcgt gggcatgaga caggccattc gcgatcagtg catcgtcatc accgtctttt
     2881 ccatagcggc tatcaggctt atccagccgt acctgccgcg cggcggagcg cttgagtatt
     2941 cactgattgt caccgattgg tagcgattga taccgatcgg cacggccagc cgtataggcg
     3001 gcgtggtcag gtatatgcaa caacgcattt atggaacacc gtttttggcg ttgccactgg
     3061 gatttccttc gatcaataac tttaccgcga gcggactcca ttcacttggg ctcaatgctt
     3121 tcgcgggttc ccctcgcaat ctgtaattga cagaacgccg gacgctttag aaggacgaat
     3181 atcttatgga cgaaaggaag cgagatagca tgattgcgta tctccgccac cgcatggaag
     3241 agtttggtat caaaccagaa gacctcgccg cggtgctggc ggccgaaccg aaggggcaaa
     3301 aggctgagcg ctaccgcagt gcaacgggag atagctggga cggccagggc gaaatgccgc
     3361 aatggcttaa acaggcaatc agtgcaggtc aatcaattga ccatttcgaa ttaacggccg
     3421 cgccagcccc cgctccgcag ccgagaaagc aggtggattg gctgaacgac ccgttcgccg
     3481 gcagcccgct cgcgcgcccg aacaatcgct aagccgtcag gctgttaaag cgccggcgcg
     3541 aagacatata accgcacttt cctgacggca acaggaggtc ggcacgttcg tcgacgctgt
     3601 caaagcgcgg acggatattc actgcgcctc gtcagcacaa gccggcgatc cgacgctcgc
     3661 ggcagcgtct cgcgccaccg cgtactgcgg cagttcgatg aatgtgctga gttgccgcac
     3721 gatttgccag aagacctttt cgaacaaacg gactcgttcc ggctcgggca tttcgtcggc
     3781 gagcggatcg gcaaaagtcc agagacacat tgaaggttca cccggaaaga gcggcgcacg
     3841 atccccggcg acacccgcat ccatggcaat gacgaggtcc attcgcggcg cccattcgcc
     3901 ggtgaattcc agccagcttt tcggactgag cacgccgagg tctgatacgc ccggccgcaa
     3961 ttgcgcgatc gcaatgggat gaacgcgcgc ggcgggctcc ggccccgcgc tgaatgcgtc
     4021 gaaccggtgg ccggccagtt cgcgcagtaa agcttcggcc atgatgctgc gtgccgaatt
     4081 ctcgcgacag agaaacagca ccttgtattt tctgctcacg ctcaccccgt atttatgttt
     4141 tttttgtctc tctacgcagt ctgcaggtcc agtcttgcgc tctcgtgaca ctacgatggc
     4201 ctaatttgtt gcgctacgag aatttgtacc aggacggtca attcgcgggt atcgcgggtg
     4261 gcatttgcga cgaagtgtag cagcgcaaag tcgcatcttt cagtatgctg gcagacgcgc
     4321 gcccgccagg cgacctccgc ggtaaccgca ggcgtgcatt gaaaaatccc acagcaacta
     4381 gagacacatg aacgctacaa cgctgaaccc tgccgccgac cgcgacaaac tggagtccct
     4441 cgcttacgcc aaagtcacct ggcgcctctt gccgtttttg tttctctgct acgtcgcggc
     4501 ctacctcgac cgcgtcaacg ttggctttgc taagctgcaa atgctcaatg acctgcactt
     4561 cagcgacacg gtttacggac tgggcgcggg tattttcttc atcggctatt tctttttcga
     4621 agtgcccagc aatgttctga tgcaccgttt tggcgcacgg gtgtggatca gccgcatcat
     4681 gatcacatgg gcgctcatct cagccgccac cttgttcgtg accacgccaa cccagttcta
     4741 cgcgttgcga ttgctgctcg gtatcgcgga ggccggcttc ttcccaggca ttgtgctgta
     4801 cctcacgtac tggtatccga gtgcgcgccg tagccgcgtc aatgcgctgt tcatggtggg
     4861 ga
//
LOCUS       DATASH010000275         8481 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3003941,
            whole genome shotgun sequence.
ACCESSION   DATASH010000275 DATASH010000000
VERSION     DATASH010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 8481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8481
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3003941"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VHV99_07705"
     CDS             complement(<1..264)
                     /locus_tag="VHV99_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011325254.1"
                     /note="catalyzes amidations at positions B, D, E, and G on
                     adenosylcobyrinic A,C-diamide. NH(2) groups are provided
                     by glutamine, and one molecule of ATP is hydrogenolyzed
                     for each amidation; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase CobQ"
                     /protein_id="HEX3635358.1"
                     /translation="MIQGTTSDAGKSTLVAGLCRLARRAGVRVAPFKPQNMALNSAVT
                     VDGGEIGRAQALQAVAAGIDAHTDLNPVLLKPTSDRGAQVIIHG"
     gene            517..1071
                     /gene="cobU"
                     /locus_tag="VHV99_07710"
     CDS             517..1071
                     /gene="cobU"
                     /locus_tag="VHV99_07710"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HEX3635359.1"
                     /translation="MIPRDLTFVLGGARSGKSVHAEQLANQSALPVTYVATARVADDA
                     EFSARIAHHRERRPAHWQLLEAGIDLAGAVAQIDAPGQCMLIDCLTLWLANLLCPPDG
                     DGLPVEHYHAHFTAFEAALINAKGKIIVVSNEIGLGVVPLGAETRLYVDELGRLNQRI
                     AALSTQVTMMVAGLPLALKTAGSA"
     gene            1071..2006
                     /gene="cbiB"
                     /locus_tag="VHV99_07715"
     CDS             1071..2006
                     /gene="cbiB"
                     /locus_tag="VHV99_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HEX3635360.1"
                     /translation="MLSLPLIVTLATAGVAVDRWLGEPRTAHPLVGFGKWATRLEARY
                     NTGRRLRLKGLLAWSLAVMPPVLIAWWLVAVLPFFWACAVHVVLLWFALGARSLHDHI
                     APIAQALARRDLAQARLLTSRIVSRETAHADEAALSRAAVESALENGNDAIFGALFWF
                     AIAGGPGALGFRLANTLDAMWGYRTPRYLRFGWAAARIDDVLNWIPARLTATSYALLG
                     DTRTALRCWREQAPRWDSPNAGPVMASGAGSLNVLIGGPAVYHGALEHRPTLGFGHAA
                     KATHVTAALLLVERTVILWLAVLIVLALLSVPFHG"
     gene            1999..3036
                     /gene="cobD"
                     /locus_tag="VHV99_07720"
     CDS             1999..3036
                     /gene="cobD"
                     /locus_tag="VHV99_07720"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase CobD"
                     /protein_id="HEX3635361.1"
                     /translation="MVERMAGHTADTGVAPDVVIHGGNLHEAAQRYGIPYAQWLDLST
                     GINPHGYPVPPVPADAWRRLPDAGDGLAACAARYYGAPDAAHVLPVAGSQAAIRALPS
                     LLPRAEVGIAPLTYSEYAPAFERAGHQVAPLDVTWDALPEAFRHVVVVNPNNPTAEHL
                     NVGKLLHWHAQLSARGGSLLVDEAFADTMPAASLAASTHRDGLIVLRSPGKFFGLAGV
                     RAGFVLGAPALLEALGRTLGAWTVSGPARHAVKAAFQDTAWQQQMRTRLDAESARLVS
                     LLHAQGLAARSTPLFAWTDDARAAAVHEALARRGVWTRLFAASGSVRFGLPASEAEWA
                     RFEEGLREAVR"
     gene            3033..3944
                     /locus_tag="VHV99_07725"
     CDS             3033..3944
                     /locus_tag="VHV99_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HEX3635362.1"
                     /translation="MIRGPRCAIAAALFAGTLHAHAAITVTDDTGATVTLSAPAQRVV
                     SLAPHVTELLYAAGGGAKLVGAVSYSDYPPEAKQLPRVGDNKALDLERIIALKPDLIV
                     VWRHGNAQRQLDRLREMHVPLFFSEPHHLDDVATSLTRLGQLLGTSPSADAAAAAYRQ
                     AIARLRTQYAGRPPVSVFYQVWDQPLMTLNGEHMVSDVIALCGGRNVFAKLEPLAPTV
                     STEAVLAANPEAIFTAAPGATKPDTTLPQLDTWRAWPSLKAVANNNLFAIDGDLINRP
                     APRIAQGAKQMCEDLDLARSRRKGGAQ"
     gene            complement(3973..5388)
                     /locus_tag="VHV99_07730"
     CDS             complement(3973..5388)
                     /locus_tag="VHV99_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEX3635363.1"
                     /translation="MNSSGIRQELDLIEQLIAASAEGRGVRALAAALAVRRHPMQRRT
                     LQRRLDALLEAGRIISEGAGRAVAYKQASVSSAAVLPTQVVTSASTSGESYVPVSSAG
                     LEVRAEVNRPLHERRPVGYNRAFLERYRPNQDAYLSQAQRARLHEWGRTSTDVRPAGT
                     YARDIMSRLLIDLSWASSRLEGNTYTRLDTVRLIEQGHIAEGKDAQETQMILNHKAAI
                     EMIVESADEVGFNRFTVLNLHAILSDNLMSDPFASGRLRAREVEISGTVFFPLAVPQQ
                     IAECFEAVLSKAAQIDDPFEQAFFGMVHLPYLQPFEDVNKRVSRLTANIPLIRQNLCP
                     LSFIDVPEKAYVDGTLGVYEANRVELLRDVFIWAYERSCQRYLAIRQSIGEPDAFRLK
                     YRNALIETVQALIRGGLAGTRGEIETLARNLVAAEDISSFIDAIQSDLDHLYLGNVAR
                     YRLRLSEFERWPFKRSETTSR"
     gene            complement(5596..6186)
                     /gene="cobC"
                     /locus_tag="VHV99_07735"
     CDS             complement(5596..6186)
                     /gene="cobC"
                     /locus_tag="VHV99_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ribazole phosphatase"
                     /protein_id="HEX3635364.1"
                     /translation="MDLVLIRHPAVALDSGVCYGHSDVALAEDAEVSSTALALKLATL
                     QVPAPRVFMSSPLMRCSALAAAMANDFGCALCHDDRLKEMNFGAWEQQRWDAIDRDLL
                     DDWAANFEHARAHGGESVAQFAARVRAWFEAFAQTRELSPAYVVTHAGVMRVIASLVL
                     DVPLERCLRWSLEMSGIVWLRRDEEAQQWSLVRWNA"
     gene            complement(6171..6947)
                     /locus_tag="VHV99_07740"
     CDS             complement(6171..6947)
                     /locus_tag="VHV99_07740"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HEX3635365.1"
                     /translation="MNPLAELRYFFTALGYFTRVPVPRWVGYEPHYLNAAARYFPLIG
                     LLIGGLSALVYLAALHLFPAGVAVLLSMAVSLLVTGAFHEDGLADCVDAFGGAYTRED
                     VLRIMHDSRIGAFGAIALVIALALKWQTLAALPPLRAASLMIAAHAASRVFAISYLAT
                     LDYVRAEGKAKPVAQRLTGPALLCAALCGLPLLLWPNGPGLPDWRFAGVTFVVLAALR
                     FAMGRYFVKRIGGYTGDCLGFAQQIFELSIYLLGLIWISS"
     gene            complement(7116..8177)
                     /gene="cobT"
                     /locus_tag="VHV99_07745"
     CDS             complement(7116..8177)
                     /gene="cobT"
                     /locus_tag="VHV99_07745"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HEX3635366.1"
                     /translation="MTSLPGLQGLTEVEPLDQTLRADLQHIIDTKTKPPGSLGRLETL
                     ARQMGLIQRTTHPTVERPAMIVFAGDHGIAQEGVSPYPQAVTAQMVANFLAGGAAINA
                     LSRVSGIELEVVNAGIATPLPSADGLIDIALAAGTRNFAHEPAMTREQALAAIQAGAA
                     RVRHHAAFGTNVIGFGEMGIANTSAAACLMSRLCGVPIDECVGRGTGLDNAGLAKKRN
                     VLASALAHHAVSNDPLDVLATFGGFEIAMMTGAYLAAAQARMTILVDGFIATSALLVA
                     DAFAAEVREYCVFAHASNEAGHRRMLDHFGALPLLSLDMRLGEGTGAALAVPLLRAAV
                     AFVNEMASFESAGVANRDA"
     gene            complement(8212..>8481)
                     /locus_tag="VHV99_07750"
     CDS             complement(8212..>8481)
                     /locus_tag="VHV99_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3635367.1"
                     /translation="TAWTHEPRRTVMFSCHDLNLARRFATHALLLDGNGGAYAGPVHD
                     VLTPTLTSRAFGYPLILIRDGEHEALIPAPRSRHESPAADDMPAG"
BASE COUNT         1561 a         2806 c         2681 g         1433 t
ORIGIN      
        1 gccgtggatg atgacctgcg cgccgcgatc gctggtcggc ttcagcagca ccgggttcag
       61 gtcggtgtgc gcgtcgatgc ccgcggccac cgcctgcaaa gcctgcgcgc gaccgatctc
      121 gccgccgtcg accgtcaccg cgctattgag cgccatgttc tgcggcttga acggcgcgac
      181 ccgcacgccg gcgcggcgcg ccagccggca caaacccgcg acgagcgtgc tcttgcccgc
      241 gtcggaggtc gtgccctgaa tcatcagcgt gccgcgcggc acgggtacag cgtcatgcgg
      301 ggtcgaagtg gtggtcacgg aatgtcggat tcggcggtga ataggcagtg aaatacgcag
      361 tgaagcagcg gcgcagcgcg caacccggcg gcaacgatat cgccgtacgc gacggcacga
      421 tacggcaccg tacagccacg aagcggcaac gccgcgattt gaactcgcca ttatcccatc
      481 gtgccggcac gcgctgcacg ccggtacaat cacgcgatga ttccccgcga cctcactttc
      541 gttctcggcg gcgcccgctc gggcaagagc gtgcacgccg aacaactggc caaccagagc
      601 gccctccccg tcacctacgt cgccaccgcg cgcgtggccg acgacgccga attcagcgcg
      661 cgtatcgcgc atcatcgcga gcggcgcccg gcacattggc aactgctcga agcgggcatc
      721 gatctcgccg gcgccgtcgc gcaaatcgac gcgcccggcc agtgcatgct gatcgactgc
      781 ctgacactgt ggctcgccaa tctgctgtgc cctcctgatg gcgacggttt gccagtcgag
      841 cactatcacg cgcactttac cgctttcgaa gcagcgctca tcaacgccaa aggcaagatc
      901 atcgtggtca gcaatgaaat cggcttgggc gtcgtcccgc tcggcgccga aacgcgcctc
      961 tatgtcgacg aactcgggcg gctcaaccag cgcatcgccg cgttgagcac ccaggtcacg
     1021 atgatggtcg cgggcttgcc gctcgccttg aaaacagcgg gcagcgcgta atgctgtcgc
     1081 ttcccctgat cgttaccctg gcaacggcgg gcgtcgccgt cgaccgttgg ctcggtgagc
     1141 cgcgcaccgc gcatccgctg gtcggcttcg gcaaatgggc gacgcgcctc gaagcgcgct
     1201 acaacaccgg ccggcgcttg cggctcaaag ggctgctcgc gtggtcgctg gcggtcatgc
     1261 cgccggtgct gatcgcctgg tggctcgtcg ccgtgctgcc gtttttttgg gcctgcgcgg
     1321 tgcatgtcgt gttgctgtgg tttgccctcg gcgcgcgcag tctgcacgat cacatcgcgc
     1381 cgatcgccca ggcattggcg cgacgcgatc tggcgcaagc gcgtcttctg acctcacgca
     1441 tcgtgtcgcg cgaaaccgca catgccgacg aagcggcgct ctcccgcgcc gccgtcgaat
     1501 cggcactcga aaacggcaac gacgcgattt tcggcgcgct gttctggttc gcgatcgccg
     1561 gcggcccggg cgcgttgggc tttcgtctgg ccaacacgct cgatgccatg tggggctacc
     1621 gcacgccgcg ctatttgcgc ttcggctggg ccgccgcgcg gatcgacgat gtgctcaact
     1681 ggattcccgc gcgcctcacc gcgaccagct atgccttgct cggggacacg cgcacggcgc
     1741 tgcgctgctg gcgcgaacag gcgccgcgct gggacagtcc gaacgccggg ccggtcatgg
     1801 cgtcgggcgc gggcagtctg aacgtattga tcggcggccc cgcggtatat cacggcgcgc
     1861 ttgaacacag accgacactc ggtttcggac acgccgcgaa ggccacgcat gtgacggcgg
     1921 cgttgctgct ggtcgaacgc acggtgattc tctggctggc ggtgctgatc gtgctggctt
     1981 tgctgagtgt gccctttcat ggttgagcga atggcgggtc acacggccga taccggcgtc
     2041 gcgcccgacg tggtgattca cggcggcaat ctgcacgaag cggctcagcg ttacggcatt
     2101 ccgtacgcgc aatggctcga cctgtcgacg ggtatcaatc cgcacggcta tccggtgccg
     2161 ccggttcccg ccgacgcctg gcgccgcctg cccgacgcag gcgacggatt ggcggcctgc
     2221 gccgcgcgct actatggcgc gcccgatgcc gcgcacgtgc tccccgtagc cgggagtcag
     2281 gcggcgatcc gcgcgctgcc ctcgctcttg ccacgcgccg aggttggcat cgcgccgctc
     2341 acctatagcg aatatgcgcc cgccttcgag cgggccggac accaggtcgc gccactggat
     2401 gtgacttggg acgccttgcc cgaggcattc aggcatgtgg ttgtcgtgaa tccgaacaat
     2461 ccgaccgccg agcatctgaa cgtcggcaag ctgctgcact ggcacgcgca actcagcgcg
     2521 cgcggcggca gcctgctcgt cgacgaggcc ttcgccgata cgatgcctgc ggcatcgctc
     2581 gcggcaagca cgcatcgcga cggcttgatc gtgctgcgtt cgccggggaa attctttggg
     2641 ctcgccggtg tgcgcgcggg cttcgtactc ggtgcgccgg ctttgctcga ggcgttgggc
     2701 cgtacgctcg gggcgtggac ggtcagcggc ccggctcgtc acgccgtcaa ggccgcgttc
     2761 caagacacgg cgtggcagca gcagatgcgc acgcgcctcg atgccgaaag cgcccgtctg
     2821 gtcagtctgt tgcatgcaca aggtctcgcc gcgcgcagca cgccgctttt cgcgtggacc
     2881 gacgacgcgc gcgccgccgc cgtgcatgaa gcgttagcgc gacgcggtgt gtggacacgg
     2941 ctcttcgcag catcgggcag cgtgcgcttc gggctgccgg cgagcgaggc ggaatgggcg
     3001 cgttttgaag aaggtttgcg cgaggcggtt cgatgattcg aggtccacgt tgcgcgatcg
     3061 ccgccgcgct tttcgcgggc acactgcacg cgcacgccgc catcaccgtc accgacgaca
     3121 ccggcgcaac cgtcaccttg tccgcacccg cgcaacgcgt cgtcagtctc gcgccacatg
     3181 tcaccgaact gctgtatgcc gcgggcggtg gcgcgaaact cgtcggcgcc gtgtcctaca
     3241 gcgactatcc gcctgaagcg aagcaactgc cgcgcgtcgg cgacaacaaa gcgctcgacc
     3301 tcgaacggat cattgcgttg aagcccgatc tgatcgtcgt gtggcgacac ggcaatgcgc
     3361 agcgtcagct cgatcgactg cgcgagatgc acgtgccgct gttctttagc gagccgcatc
     3421 atctcgacga cgtcgccacg tcgctgacca gactggggca attgctcggc acctcgccga
     3481 gcgccgatgc cgccgccgcg gcataccgtc aggccatcgc gcgcttgcgc acgcagtatg
     3541 cgggcaggcc gccggtcagc gtgttctatc aggtgtggga tcaaccgttg atgacgctca
     3601 acggcgagca tatggtgagc gatgtgatcg cactatgcgg cggccgcaat gtgtttgcga
     3661 aactcgaacc gctcgcgccg accgtctcga ccgaagcggt gctcgcagcg aatcccgaag
     3721 cgatcttcac tgccgcgccc ggcgcgacca aaccggacac gactttgccg caactcgaca
     3781 catggcgtgc gtggccgagc ctcaaagcgg tcgcgaacaa caatctgttc gccatcgacg
     3841 gcgatctgat caaccggccg gcgccgcgga ttgcgcaagg ggcgaagcag atgtgcgagg
     3901 acctggacct ggcgcgctca cggaggaagg gcggcgcgca gtgatgcacg tcgcgaaacc
     3961 gaaaatgaag cctcagcgtg aggtcgtctc ggaacgcttg aacggccagc gctcgaattc
     4021 agaaagccgc aaccggtaac gcgcgacgtt gccaaggtaa aggtgatcga gatcggactg
     4081 gatcgcgtcg atgaacgagg agatatcctc cgcggccacc aggttccgtg cgagcgtttc
     4141 gatctcacca cgcgtgccgg caagaccgcc gcgaatcaac gcctgtaccg tctctatcaa
     4201 cgcgttgcga tacttgaggc ggaacgcgtc cggctcgccg atggattgtc gaatcgccag
     4261 ataacgctga cacgagcgct cgtacgccca gatgaatacg tcgcgcaaca actcgacgcg
     4321 attggcttcg tacacgccga gcgtaccgtc aacgtaggcc ttttcgggaa cgtcgatgaa
     4381 cgacagcgga cacagattct gccggatcag cgggatattc gcggtcagac gcgagacccg
     4441 tttgttgacg tcttcgaacg gctgcagata cggcaggtgc accatgccga agaaagcctg
     4501 ttcgaaggga tcgtcgatct gggcggcctt gctcaatacc gcctcgaagc attccgcaat
     4561 ctgttgcggc acggcgagcg gaaaaaagac ggtgccgctg atctccactt cgcgcgcgcg
     4621 caaacggccg ctggcgaacg ggtcggacat caggttatcg gagagaatcg cgtgcaggtt
     4681 caggacggtg aagcgattga agcccacttc gtccgcgctc tcgacgatca tttcgatcgc
     4741 ggctttgtga ttcaggatca tctgcgtttc ctgagcgtcc ttgccttccg caatgtgccc
     4801 ttgttcgatc aaccgtacgg tatcgagccg cgtgtacgta ttgccctcga gccgcgacga
     4861 cgcccacgac agatcgatca gaagccggct catgatgtcg cgcgcgtaag tacctgccgg
     4921 ccttacatcc gtcgacgtgc ggccccattc gtgcaggcgt gcacgctgcg cttgcgacag
     4981 gtacgcgtcc tgattcggac gatagcgctc gagaaacgcg cggttatagc cgaccggccg
     5041 gcgctcgtgc agcggcctgt ttacttccgc gcgcacctcg agacccgcgc tcgacacggg
     5101 cacgtaagac tcgccggacg tgcttgcgct ggtgacaacc tgggtaggaa gaacagccgc
     5161 gctggaaact gaagcctgct tgtaggcgac ggcccggccg gcaccttccg agatgatccg
     5221 gcccgcttcg agcaacgcgt cgagacgacg ttgcagcgtg cggcgctgca tgggatgacg
     5281 gcgcacggca agtgccgcgg caagcgcgcg aacgccccgc ccctcagcgc ttgccgcgat
     5341 gagttgctcg atcaaatcca gttcctgcct gataccgctg ctgttcatgc gcgccaccct
     5401 tgagcctatg tggcgcgata agttctaatc gtgccacttt ctcatggcat tgtggcacaa
     5461 ttcggcctaa tcgcgccact tttccactta caagtggcgc gattatggtt ttcgtgacac
     5521 attttcagaa aatgtgtcac gaataacgcc gagactgacg caattccagg gaggcggcag
     5581 acgcaagccg ggtcctcacg cattccacct caccaacgac cactgctgcg cctcctcgtc
     5641 cctgcgcagc cagacgatcc ccgacatctc cagcgaccat cgcagacagc gctccagcgg
     5701 cacatccagc accagcgacg caatgacccg catcacaccg gcatgggtga ccacatacgc
     5761 cggcgacaac tcccgcgtct gcgcgaatgc ctcgaaccac gcccgcaccc gcgcggcgaa
     5821 ttgcgcgacg ctctcgccgc catgcgcacg cgcgtgctcg aagttggccg cccaatcgtc
     5881 gagcaaatcg cgatcgatag catcccagcg ctgctgctcc caggcgccga agttcatttc
     5941 cttcagacga tcgtcatggc agagcgcgca accgaaatcg ttcgccatcg cggcggcgag
     6001 cgccgagcag cgcatcaacg gactcgacat gaacacgcgc ggcgccggca cctgcaacgt
     6061 ggcgagcttc aatgcgagcg cggttgaaga cacctccgca tcctcggcaa gcgcgacgtc
     6121 gctatggcca tagcacacgc ccgaatcgag cgcgacggcg ggatggcgaa tcaggacgag
     6181 atccatatca gccccagcag atagatgctc aattcaaaga tctgttgtgc aaagccgagg
     6241 cagtcgccgg tgtagccgcc aatgcgtttg acgaagtaac ggcccatggc aaaccgcaac
     6301 gccgcgagta cgacgaatgt caccccggcg aagcgccaat ccggcagacc cggtccgttc
     6361 ggccagagca acagcggcaa accgcacagc gccgcgcaca gcagcgcggg accggtcaag
     6421 cgctgcgcaa ccggcttggc cttgccctcc gcgcgcacgt agtcgagcgt ggccagatag
     6481 ctgattgcaa acacgcggct tgcggcatgc gcggcaatca tcagacttgc cgcgcgcagc
     6541 ggcggcagcg cggcgagcgt ttgccacttc aatgcaagcg cgatcacgag cgcgatggcg
     6601 ccgaacgcac cgatgcgcga atcatgcatg atgcgcagca cgtcctcgcg tgtataggcg
     6661 ccaccgaacg catcgacgca atcagcgagc ccgtcttcgt gaaatgcgcc agtgacgagc
     6721 agcgacaccg ccatcgacag cagcaccgcc acgcccgctg gaaagagatg cagcgccgcg
     6781 agatagacca gcgcgctcaa tccgccgatc aatagaccga tgagcggaaa atatcgcgcc
     6841 gccgcattca gatagtgcgg ctcatagccg acccaacgcg gcaccggcac gcgcgtgaaa
     6901 tagccaagcg ccgtgaagaa atagcgcagt tccgcgagcg ggttcatgag tgagcgaggc
     6961 gcacgttttt gtgaggggga gtctcgtatg aaggcacctc gtatgaaagc gccttgtctg
     7021 aaagtgcctt gcctgaaagt gcctcgtatg aaggtacctt gcatgaaagc gcctcggtta
     7081 aaagcgcctc ggataacgac gcttcgtata gcggcttacg catcgcgatt cgcgacaccg
     7141 gccgattcga agctcgccat ttcgttgacg aacgccactg ccgcgcgcag cagcggcacc
     7201 gcgagcgccg cgccggtgcc ttcgcccaga cgcatatcga gcgagagcaa cggcagcgcg
     7261 ccgaaatgat cgagcatgcg ccgatgcccc gcctcgttcg acgcatgcgc gaacacacaa
     7321 tactcgcgca cctccgctgc gaacgcatcg gccaccagca aggccgaggt cgcgatgaaa
     7381 ccgtcgacga ggatcgtcat gcgggcttgc gcggcagcga gatacgcacc cgtcatcatc
     7441 gcgatttcga agccaccaaa ggtggcgagc acatcgagcg gatcgttgga aacggcgtga
     7501 tgcgcgagcg ccgatgcgag tacgttgcgc tttttcgcga ggcccgcatt gtcgagaccg
     7561 gtcccgcggc cgacgcattc gtcgatcggc acgccgcaca ggcggctcat cagacacgcg
     7621 gccgccgacg tattcgcaat gcccatttcg ccaaagccga tcacattggt gccgaacgcc
     7681 gcgtgatgcc gcacgcgagc cgcgcccgcc tgtatggcgg caagcgcctg ttcacgcgtc
     7741 attgccggtt cgtgcgcgaa gttgcgcgtg ccggccgcaa gcgcaatgtc gatgagaccg
     7801 tcggccgacg gcaggggtgt cgcaatgcct gcgttgacca cctcgagctc gatgccggac
     7861 acgcggctca aggcattgat cgccgcgccg cccgcgagaa agttggcgac catttgtgcg
     7921 gtcaccgcct gcggataagg actcacgcct tcctgcgcaa tcccgtggtc accggcaaac
     7981 acgatcatcg ccggacgttc gaccgtgggg tgcgtcgtac gctggatcaa tcccatctgg
     8041 cgcgcgagtg tctcgagccg cccaagactg ccgggcggtt tggtcttggt atcgatgatg
     8101 tgttgcaggt cggcacgcag cgtttgatcg agcggttcga cttcggtcaa tccctgcaaa
     8161 ccaggcaaag aagtcataga ggtttctaaa agagagtgtt ggttaaagcg cttagcctgc
     8221 cggcatgtca tcagccgcag gcgattcgtg acgcgagcgc ggcgcgggaa tcagcgcctc
     8281 atgctcgccg tcgcgaatca gaatcaacgg atagccgaac gcgcggcttg tcagcgtggg
     8341 cgtcagcacg tcgtggaccg gtccggcata cgcgccgccg ttgccgtcga gcagcaacgc
     8401 atgggtcgcg aagcggcgcg cgaggttcag gtcgtggcac gaaaacatca ctgtacgccg
     8461 aggctcgtgg gtccaggccg t
//
LOCUS       DATASH010000276         2470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1224531,
            whole genome shotgun sequence.
ACCESSION   DATASH010000276 DATASH010000000
VERSION     DATASH010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1224531"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VHV99_07755"
     CDS             <1..375
                     /locus_tag="VHV99_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEX3635368.1"
                     /translation="GWWAPLLLGLCSVAAGVIAIFLPAITALVLVLVMGVNAIATGVF
                     DLLTAFRLRGHIRGTWPLYLSGILSLVFGVLVLAWPGAGALALVWIIGAYAIFTGALL
                     LVLGLSARGWLRDGLTGHPSAG"
     gene            417..1028
                     /locus_tag="VHV99_07760"
     CDS             417..1028
                     /locus_tag="VHV99_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX3635369.1"
                     /translation="MSLDLDKWNPFRFLRHSADEKQRQASSRVPQQHQQGTQREVGVQ
                     PSGTAALPSISADPARWPLADPFQLFTDLIRDPFGGLGPLEQWFGDFSPSRFQPRIDV
                     VDDGDALRIVAELPGLGRDDVKLEVVENMLVLSGNKQIESKGEEKGCYRVERAFGQFQ
                     RAIPLPSGVDPDRAEARFENGVLTVRMPKTEQAQQTTKQIQIK"
     gene            1119..>2470
                     /locus_tag="VHV99_07765"
     CDS             1119..>2470
                     /locus_tag="VHV99_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEX3635370.1"
                     /translation="MAKCSVCGKPATTQITITENGQRRQLMLCDEHHMEFMARHQRSL
                     SPLESLFHGSLFDRLFDEGWPPFESSDTDRPQPGRSVTGRAASGSAGTAASAGETGDG
                     SGSARPRRRRRAREAVDLQTFLSESGLDRLQAAAQKAVEFGKNEVDTEHLLLALADNN
                     VVREILREFKLDADEIKHSVEQDAPRGTRTGENDESRQIGVSPRAKSALENALVASRE
                     LDHSYVGPEHILIGLVEEEDGFAGELLRKLGLTSQAMRQKAVKVVGQGAEGGSVEGRS
                     TTPTLDKYARDLSELARRGKLDPVIGRDKEIETTIEVLARRRKNNPVLIGEPGVGKTA
                     IVEGLAQRIVQDQIPEVLRGKRVVELNLNSVVAGAKYRGEFEERVKQVLDEIVANQDR
                     LIIFIDELHTIVGAGQGGGEGGLDIGNVFKPALARGELHLIGATTLNEYQKYIERDSA
                     L"
BASE COUNT          487 a          771 c          806 g          406 t
ORIGIN      
        1 ggctggtggg cgccgctgct gctgggactg tgcagcgtcg ccgccggggt gatcgcgatc
       61 ttcctgcccg cgatcaccgc cctcgtgctc gtcctcgtga tgggcgtcaa tgccatcgcg
      121 acgggcgtat tcgacctcct caccgccttc cggctgcgcg gtcatattcg cggcacctgg
      181 ccgctgtacc tgagcggcat tctttccctg gtgttcggcg tgctcgtact cgcgtggccc
      241 ggcgccggtg cgctcgcgct cgtctggatc atcggtgcgt atgccatttt caccggcgcg
      301 ttgctgctgg tgctcggcct cagtgcgcgg ggctggctcc gcgacgggct gacgggccat
      361 ccgtccgccg gatgaagacg tgcaccgttt aactcatttc atcaaaggag tcagtcatgt
      421 cactcgatct cgacaagtgg aacccgttca ggtttctacg tcattccgcc gatgagaaac
      481 agaggcaggc ttcgtctcgc gtgccgcaac agcaccagca gggcacccag cgagaagttg
      541 gtgtgcagcc ttcaggcacc gccgccttgc cgtcgatatc ggccgatcca gcgcgctggc
      601 cgctggccga tcctttccaa ctattcaccg atctgatccg cgatccgttc ggtggcctcg
      661 gtccgctcga acagtggttc ggcgacttca gcccgagccg gttccagccg cgcatcgacg
      721 tggtcgacga cggcgacgcg ctgcgtatcg tcgccgaact gccaggcctg ggccgcgacg
      781 acgtgaaact cgaagtagtc gagaacatgc tggtgctgag cggcaacaag cagatcgaat
      841 ccaaagggga agaaaaaggc tgttaccggg tagaacgcgc gttcggccaa tttcagcgcg
      901 cgattccgct gccgtccggc gtcgatcccg atcgcgccga agcgcgcttc gagaatggcg
      961 tgctgacggt acgcatgccc aagacggagc aggcacagca aaccacgaag cagatccaga
     1021 tcaagtgacg ccgggcaccc gtgcgtcagc tacccaggaa tcgacagcgg cacgcatcgc
     1081 ggcgcgcagc gggcgggcag aacctgaaga ggaacatcat ggccaagtgc agtgtttgcg
     1141 gtaaaccggc gacgacgcag attacgatca ccgagaatgg ccagcgccgg cagttgatgc
     1201 tgtgcgacga acatcacatg gagttcatgg cacgtcacca gcgcagtctg tcgccgcttg
     1261 aatcgctttt tcacgggagc ctgttcgacc ggctgttcga cgaaggatgg ccgccgttcg
     1321 aaagcagcga cactgacagg ccgcagcccg gccgctccgt gacgggccgc gcggcgtctg
     1381 gttcggcggg gacggcagcc tcagccggcg aaactggcga tggcagcggg agcgcccgtc
     1441 cgcgccggcg ccgccgcgca cgcgaagccg tcgatctgca gacgtttctg agcgagagcg
     1501 ggctcgaccg cctgcaggcc gccgcgcaga aagcggtcga attcggcaag aacgaagtag
     1561 acaccgaaca tctgctgctc gcgctggcgg ataacaatgt cgttcgggaa atcctgcgcg
     1621 agttcaagct cgacgcagac gaaatcaaac actccgtcga gcaggacgcg ccacgcggca
     1681 cacgcaccgg tgaaaacgac gagagccgcc agatcggcgt atcgccgcgt gcgaagagcg
     1741 cgctcgaaaa cgcgctggtt gcgtcacgcg aactcgatca tagctacgtc ggtccggaac
     1801 acatcctgat cggcctcgtc gaggaagaag acggtttcgc gggcgagttg ctgcgcaagc
     1861 tcgggctgac gtcgcaggcg atgcgtcaaa aggcggtgaa agtggtcggc cagggcgcgg
     1921 aaggcggcag cgtcgaaggc cgttccacca cgcctacgct cgataaatac gcgcgcgatc
     1981 tcagcgaact ggcacggcgc ggcaagcttg atccggtgat cggacgcgac aaggagatcg
     2041 aaaccaccat cgaagtgctc gcgcggcgcc gcaagaacaa tccagtcctg atcggtgaac
     2101 cgggtgtcgg caagacggcg atcgtcgaag gcctcgcgca acgcatcgtg caggatcaga
     2161 ttccggaagt actgcgcggc aagcgggttg tcgaactcaa tctcaacagt gtcgtggcag
     2221 gagcgaaata tcgcggcgaa ttcgaggagc gcgtcaagca ggtgctcgac gagatcgtcg
     2281 cgaaccagga ccggctgatc atcttcatcg acgagcttca tacgatcgtc ggggccggcc
     2341 agggcggcgg cgaaggcggc ctcgacatcg gcaacgtgtt caagccggca ctcgcgcgcg
     2401 gcgaactgca tctgatcggc gcgaccacgc tcaacgagta ccagaagtac atcgaaaggg
     2461 attcggcgct
//
LOCUS       DATASH010000277         2723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3203381,
            whole genome shotgun sequence.
ACCESSION   DATASH010000277 DATASH010000000
VERSION     DATASH010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3203381"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..716)
                     /gene="ruvC"
                     /locus_tag="VHV99_07770"
     CDS             complement(174..716)
                     /gene="ruvC"
                     /locus_tag="VHV99_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX3635371.1"
                     /translation="MRILGIDPGLRVTGFGVIDQTGHTLSYVASGVIKTADADLPSRL
                     GTIFEGISTLIRQHSPDQSAIEKVFVNVNPQSTLLLGQARGAAICGLVAGGVPVAEYT
                     ALQLKQAVVGYGRATKEQMQQMVVRLLNLSGVPGTDAADALGMAICHAHGGTTLSTLG
                     GIAPSLAKKGLRVRRGRLVG"
     gene            850..1089
                     /locus_tag="VHV99_07775"
     CDS             850..1089
                     /locus_tag="VHV99_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635372.1"
                     /translation="MEHYWNLSGDSGVDAYEIGDDFVAVRFKPGVVYWYTKASVGAKH
                     VATLKRLARRGQGLSTYISQHVEVKEGYARKDPDD"
     gene            complement(1362..>2723)
                     /gene="purH"
                     /locus_tag="VHV99_07780"
     CDS             complement(1362..>2723)
                     /gene="purH"
                     /locus_tag="VHV99_07780"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX3635373.1"
                     /translation="KVHGGILARRDLPEHMAALEKHDIPTIDLLVVNLYPFVQTVSKE
                     ECSLEDAIENIDIGGPTMLRSAAKNHRDVTVVVDPADYAVVLDEMRANSNAMSYKTNF
                     RLATKVFAHTAQYDGAITNYLTSLTDELQHSSRNAYPATFNLAFNKVQDLRYGENPHQ
                     SAAFYRDLVVPAGALANYNQLQGKELSYNNIADSDAAWECVKTFDVPACVIVKHANPC
                     GVAVGADANEAYAKAFQTDPTSAFGGIIAFNREVDETAAQAVAKQFVEVLIAPSFSAE
                     ARQVFAAKQNVRLLEIALGEGHNAFDLKRVGGGLLVQSLDSKNVQPRELRVVTKRHPT
                     PKEMNDLLFAWRVAKYVKSNAIVFCGNGMTLGVGAGQMSRVDSARIASIKAQNAGLTL
                     TGSAVASDAFFPFRDGLDVVVAAGATCVIQPGGSMRDDEVVSAADEHNIAMVLTGVRH
                     FRH"
BASE COUNT          546 a          855 c          823 g          499 t
ORIGIN      
        1 ggtttttttc cagcagaacg ccggcaatgc gaccgatcat ggcggattca atcttgagaa
       61 agggcgagtg tagcgcagcg agtgcgcggc gggcgtggtg tacgaggaca tggttgacac
      121 tttcgggcga tgtccccgaa cggcgattcg tatcaggcgc tgccttggcg cctctacccc
      181 accaaccgtc cacgcctgac gcgcagaccc ttcttcgcca gcgaaggcgc gatgccgccc
      241 agcgtactga gcgtcgtgcc gccgtgtgca tggcagatcg ccatgccgag ggcgtcggcg
      301 gcgtcggtgc cgggtacgcc ggagaggttg agcagacgca ctaccatctg ctgcatctgc
      361 tccttggtcg cgcggccata gccgactacc gcttgcttca actgcaacgc cgtatattcg
      421 gcgaccggca cgccacccgc caccagtccg cagatcgccg cgccgcgtgc ctggccaagt
      481 agcagggtgg attgcgggtt gacgttgacg aacacctttt cgatcgccga ctgatccggc
      541 gaatgctgac ggatcagcgt cgaaatgccc tcgaaaatgg tgccgagacg cgacggcagg
      601 tcggcgtcgg cggtcttgat gacgccactt gcgacgtagc tgagcgtgtg tccggtttga
      661 tcgattacgc caaagccagt cacgcgcaag ccggggtcga tgccgagaat tctcatgagg
      721 tgccgcgtgt gcggtgaaaa ccaggtgcct gcgatactac aacgaacgca ccgcgcggcg
      781 gtcggtgtgc ggcgatgtca gtggaataaa aactgctgcc acatacgttg aaacgggagt
      841 tgatcgcata tggagcacta ctggaatctc agcggcgact caggcgttga cgcatacgaa
      901 atcggcgacg acttcgtcgc ggttcgcttt aaaccgggcg tcgtgtattg gtacacgaag
      961 gcgagcgtcg gcgcgaagca cgtcgccacg ctcaagcgac tcgcgcggcg agggcagggg
     1021 ctgagcacct atatcagcca acatgtcgag gtgaaggaag gttacgcgag aaaggacccg
     1081 gacgattgag ccgtactgct cggagaacgg ggcagcgaac gtagccaaag aaacgggcca
     1141 aagaaacgcg ccacagaaac gcgccacaga actggggcaa agagccggga caaagaaacg
     1201 cgtcacagaa acagcccggc gaatccgtcc cggagaccct atcggaaaca ccgcccaaaa
     1261 gataacggcc cggcggaaaa tccgccgggc cgtttcgctc aattcagtct ggcaagcgcc
     1321 gcatcacaaa ccagcacgca ccgctcgccc aatcgcccgc atcaatgccg gaagtgacgc
     1381 accccggtca acaccatcgc gatgttgtgc tcatccgccg cgctgaccac ttcgtcgtcg
     1441 cgcatcgagc cgcccggttg gatcacgcag gttgcgcctg ccgcgaccac cacatccaga
     1501 ccgtcacgga acgggaagaa cgcatctgac gccaccgccg aacctgtcag cgtgagaccg
     1561 gcgttttgcg ccttgatact cgcaatccgc gccgagtcca cgcggctcat ctgacccgcg
     1621 ccaacgccaa gcgtcatgcc gttgccgcaa aacacgatcg cgttcgattt cacatacttc
     1681 gcgacgcgcc acgcgaacag cagatcgttc atttccttcg gcgttggatg acgcttcgtg
     1741 accacgcgca attcgcgcgg ctgcacgttt ttcgaatcaa gcgactggac cagcaaaccg
     1801 ccgccgacgc gtttcagatc gaacgcgtta tggccttcgc ccaaagcgat ttccagcaaa
     1861 cgcacattct gcttcgccgc gaacacctgg cgggcttcag cgctgaacga cggcgcgatc
     1921 agcacttcaa cgaactgctt ggcgaccgct tgcgccgcgg tttcgtccac ttcgcggttg
     1981 aacgcgatga tgccgccgaa cgccgaagtc gggtcggtct ggaacgcctt cgcatacgct
     2041 tcgttggcat ccgcgcccac ggccacgccg cacggattcg cgtgcttcac gatcacgcaa
     2101 gccggcacgt cgaacgtctt cacgcattcc cacgccgcat cggaatcggc gatgttgttg
     2161 tacgacagtt ccttgccttg caactggttg tagttcgcca gtgcgccggc cggcacgacg
     2221 aggtcgcgat agaacgcggc gctctgatgc gggttttcgc cgtagcgcag gtcctgcacc
     2281 ttgttgaacg ccaggttgaa cgttgccggg taagcattgc gcgaagagtg ttgcaattcg
     2341 tcggtcaggc tcgtcaggta gttcgtgatc gcgccgtcgt attgcgcggt gtgcgcgaac
     2401 accttggtgg ccagacggaa gttggtcttg tacgacatcg cgttgctgtt cgcgcgcatt
     2461 tcgtcgagca cgaccgcgta atccgccgga tcgaccacca cggtcacgtc gcgatgattc
     2521 ttcgcggccg aacgcagcat ggtcgggccg ccgatgtcga tattctcgat cgcgtcttcc
     2581 agcgagcatt cttctttcga cacggtctgc acgaacggat acagattcac gaccagcagg
     2641 tcgatcgtcg ggatgtcgtg cttttcaagc gcggccatgt gttccggcag atcgcggcgc
     2701 gccagaatgc cgccgtgcac ctt
//
LOCUS       DATASH010000278         5176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6565133,
            whole genome shotgun sequence.
ACCESSION   DATASH010000278 DATASH010000000
VERSION     DATASH010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6565133"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..590)
                     /locus_tag="VHV99_07785"
     CDS             complement(171..590)
                     /locus_tag="VHV99_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX3635374.1"
                     /translation="MAMSPFAGDDDDGLMNEINMTPLVDVMLVLLIVFMVTIPVMRHA
                     VKIDLPHASSQKEDTKPAQVTVTVDADGNVLWDDRKVDDAALREKIAEAAQANPQPEL
                     HLDADRKVAYEKVAEVMSAAQAGGLTKIGFVTQPKGK"
     gene            complement(607..1338)
                     /locus_tag="VHV99_07790"
     CDS             complement(607..1338)
                     /locus_tag="VHV99_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX3635375.1"
                     /translation="MQNYGLAHVWAQGDFVTRGIALALLIMSVMSWSVIVIKGWNVMR
                     LKRLTKNAEQQFWHSDDLADGVKKLGAGSSTPQDNPFLALALSGQEAADHHHQTQPHL
                     HDRMDVSDWVTRCLKDTMDESVARMQSGLAILASIGSTAPFVGLFGTVWGIYHALLAI
                     GASGQSSIDQVAGPVGEALIMTAFGLFVAIPAVLGYNALTRANKALVAKLSRFAHGLH
                     AFFVTGARLSSSRRGDGLRLATRAN"
     gene            complement(1365..2093)
                     /locus_tag="VHV99_07795"
     CDS             complement(1365..2093)
                     /locus_tag="VHV99_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX3635376.1"
                     /translation="MRASPTVSAGVTPASSARINSRVWTATAAVAAIHVALLAVVLTL
                     RHDPVQPPLESRVMTAQLLAPAPVAAPVAVQSIAPPPPKPTPPVHTRPKVQPKPILTS
                     KPTPTPLPEAAAPSPTPVAPADPTPAAPAAPPAQAAPAVGRPTMDITAPKNVSHIECN
                     MITPDYPALSKRRAETGTAYVTFVIGPTGKVESISLKKSSGFSRLDDAAIAAAHASSC
                     KPYLENGQPIRAMATQPYNFNLND"
     gene            complement(2367..2606)
                     /locus_tag="VHV99_07800"
     CDS             complement(2367..2606)
                     /locus_tag="VHV99_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004187745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX3635377.1"
                     /translation="MIVCVCKSVSDRKIRASIAEGVDSFDELQFELGVASCCGKCADS
                     VRSVMMESGVCASRCGFDHHSQQPMPLTFYERKAA"
     gene            complement(2821..3708)
                     /gene="murI"
                     /locus_tag="VHV99_07805"
     CDS             complement(2821..3708)
                     /gene="murI"
                     /locus_tag="VHV99_07805"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEX3635378.1"
                     /translation="MPAESLSSSAGIAPSGGDSRAPVGIFDSGLGGLSVLRAVRAQLP
                     HEALIYVADSRYAPYGERDDDFIADRTLAIGEWLASQGAKALVVACNTATAQSIALVR
                     EKLTIPLVGVEPGVKPAALQSKTRVAGVLATQVTLRSARFQALLERYAADCRFLCQPG
                     HGLVQAVERCDVGSAELRALLRNYLEPMLDAGADTLVLGCTHYPFLDTAIRDIVGDRL
                     MLIDTSVAIARQLERVLDQHGLRAASENADVVLPRFYSTGDGSHQQQLATTLLQIDAA
                     VEQVVIPSRRTAAPDSQAA"
     gene            complement(3791..4270)
                     /gene="bfr"
                     /locus_tag="VHV99_07810"
     CDS             complement(3791..4270)
                     /gene="bfr"
                     /locus_tag="VHV99_07810"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEX3635379.1"
                     /translation="MQGDKKVIEYLNSQLKNELTAINQYFLHARMYKHWGLEKLGKHE
                     YDESIGEMKHADWLIERIFMLDGLPNLQDLHKLLIGEETKEILECDLKLEQISQSTCK
                     EAIVYCESVRDFISREIFVKILDDTEEHIDWLETQIDLIDKVGIQNYQQTAMGSIES"
     gene            complement(4462..>5176)
                     /locus_tag="VHV99_07815"
     CDS             complement(4462..>5176)
                     /locus_tag="VHV99_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HEX3635380.1"
                     /translation="VAIIPNCAATRHAHFTLDGSGVAKLEAPSLDAWPKVQWEPDTEK
                     SKRVDLNTLTPAEVAGWKPGQTLLLSGKMLTGRDAAHKRIADMLAKGEKLPVDFTNRV
                     IYYVGPVDPVRDEAVGPAGPTTATRMDKFTETMLSQTGLVSMIGKAERGPVAIEAIKK
                     HKAAYLMAVGGAAYLVSKAIRSAKVLAFEDLGMEAIYEFDVQDMPVTVAVDSNGTSVH
                     QTGPKEWQAKIGKIPVATV"
BASE COUNT          977 a         1542 c         1608 g         1049 t
ORIGIN      
        1 cttcgaccga tggcaaataa tcccgccgcc cgaaggcgcc gagtgcgcaa aaaaaaggcc
       61 ttcatctgac gatgaaggcc ttttactttt agcgtttgtt tcaagggttt ttctgctttt
      121 tcggcaggcg ctgcttaaca gcggcgtgct gctatgcgga tcgccggtcc ttatttgcct
      181 ttgggctgcg tgacgaagcc gatcttcgtg aggccaccgg cttgcgccgc cgacatcact
      241 tcggccactt tctcgtatgc gaccttacgg tccgcatcca gatgcagctc cggttgcgga
      301 ttggcttgcg cggcctctgc gattttctcg cgcagcgccg cgtcgtcgac cttcctgtcg
      361 tcccacaaca cgttgccgtc cgcgtcgacg gtgaccgtta cctgcgcggg tttcgtgtct
      421 tccttctgac tgctcgcatg cggcagatcg attttgaccg cgtgacgcat caccggaatc
      481 gtcaccatga agacgatcag gagtaccaac atgacgtcga cgagcggcgt catgttgatt
      541 tcgttcatca ggccgtcgtc atcgtcgccg gcgaaggggc tcattgccat cggaatgcct
      601 cgtcgatcag ttggcgcgcg tggcgaggcg caaaccgtcg ccgcgcctgg acgacgacag
      661 acgggcgccc gtcacgaaga aggcgtgcag accgtgcgca aagcggctca gcttggcgac
      721 gagtgccttg ttggcgcgcg tcaatgcgtt gtagccgagc acggccggaa tagcgacgaa
      781 cagaccgaac gcagtcatga tcagcgcttc gccaaccgga cccgcgacct gatcgatcga
      841 cgactggccg ctcgcgccga tcgccagcag tgcgtgatag atgccccaca ccgtgccgaa
      901 caggccgacg aacggcgcgg tgctgccgat cgaggcgaga atcgccagac cgctttgcat
      961 gcgcgcgacg ctttcgtcca tcgtgtcctt caggcagcgc gtcacccaat ccgacacgtc
     1021 catgcgatcg tgcagatgcg gctgcgtctg atgatgatgg tcggccgctt cctgaccgga
     1081 cagcgcgagc gcgagaaacg gattgtcctg cggcgtcgac gacccggcgc caagcttctt
     1141 cacgccatcg gcgaggtcgt ccgaatgcca gaactgctgt tcggcgttct tcgtgagacg
     1201 cttcaggcgc atcacgttcc agcccttgat gacgatcacg ctccacgaca tcaccgacat
     1261 gatcaatagc gcgagcgcaa taccgcgcgt cacgaaatcc ccttgcgccc acacgtgcgc
     1321 caatccgtag ttttgcattg ccattccttt ttttgaaagc tgcctcaatc gttcagattg
     1381 aagttgtaag gctgggtggc catggcccga atgggctggc cgttctcgag atagggcttg
     1441 cacgagctcg catgcgcggc ggcgatggcg gcgtcgtcca gacggctgaa gccgctgctc
     1501 tttttgagcg agatgctttc gaccttcccc gtcggtccga tgacgaacgt cacataggcc
     1561 gtaccggttt cggcgcggcg cttcgacagc gcggggtagt ccggcgtaat catgttgcac
     1621 tcgatgtgcg agacgttctt cggcgcggtg atgtccatcg tcggacggcc gactgccggc
     1681 gcagcctggg cgggcggtgc agccggcgcg gctggcgttg gatcagccgg ggcgaccggt
     1741 gtaggcgacg gcgcggccgc ctcgggcaac ggcgtaggcg tcggcttgga cgtcaatatc
     1801 ggctttggct gaaccttggg cctggtatga accggcggcg tcggcttggg cggtggcggc
     1861 gcgatcgatt gcaccgcaac aggcgcggcc acgggcgccg gcgccagcag ctgcgcggtc
     1921 atgacgcgcg attcgagcgg cggctgcacg gggtcatgac gcaacgtcag gaccaccgca
     1981 agcaaggcga catgaatcgc ggcgactgcg gcagtcgcgg tccagacacg ggaatttatt
     2041 cgggcggacg aagccggcgt gacgccggct gaaacggtag gcgaggcccg catattagcg
     2101 tggcgacgcg ccgtcgatgc ggtgcgtcag gcaagacatc gtcatgttcg gaagatcagc
     2161 agcgaaatga acaaagtagt cacgaaacca atcagcaatt ccatctcgaa ctccttttaa
     2221 cggatgagtg ggtagctggc ttgtacggat actggcttgc tgacggcagt gggataacac
     2281 gtgaagcacg gcgaaaagcg gcgcctgaag cagtgagcgc ccgcgcggcg tgaccgtgct
     2341 ctaaaaccgg cctcgcgttc gcaaactcag gcggctttgc gttcgtagaa cgtcagcggc
     2401 ataggctgct gcgagtgatg atcgaagccg cagcggctcg cgcatacccc gctttccatc
     2461 atgacgctgc gcacggaatc cgcgcacttg ccgcaacagg aggcgacgcc gagttcgaac
     2521 tggagttcgt cgaacgaatc gacgccttcc gcgatcgagg cacggatctt tcggtcagaa
     2581 acagacttgc agacacagac aatcatggca ggcgtcatag ctaacgttaa tgcgaactat
     2641 tatcattttg attgagccgt ttggcaagcg tcctgcatga ttttttgtaa caaaaccaga
     2701 ctcttagaag ggtttcgcct aagcgatggg atcgggggtc aggggctgaa agggttcgaa
     2761 acgccaaaaa acaacgcaaa aaggcctcgg ggcggcccca aagcacaccg cgagcggttt
     2821 ttatgcggct tgggaatccg gcgcggccgt gcggcgcgaa gggatgacaa cctgttcgac
     2881 cgccgcgtcg atctgcagca aagtcgtggc gagctgttgc tgatggctgc cgtcgcctgt
     2941 cgagtaaaag cggggcagaa caacgtcggc attttccgag gcggcacgca ggccgtgctg
     3001 gtcgagtacc cgttccagtt ggcgcgcgat ggccacgctc gtatcgatca gcatgagccg
     3061 gtcgccgacg atgtcgcgga tcgccgtatc gagaaagggg tagtgggtgc aaccgagcac
     3121 caaggtatcg gcgccggcat cgagcatggg ctcgaggtaa ttgcgcagca gcgcgcgcag
     3181 ttcggcggag cccacgtcgc agcgctccac cgcctgcacc agcccgtggc caggctggca
     3241 gagaaaacgg caatcggcgg cgtagcgctc cagcagcgcc tgaaagcggg cgctgcgcaa
     3301 ggtcacctgg gtagcgagca cgccagcgac acgcgttttc gactgcagtg cggccggttt
     3361 gacgcccggc tcgacaccca ccagcggaat cgtcagcttt tcgcggacca gtgcgatcga
     3421 ctgcgcggtc gccgtgttgc atgcgaccac cagggccttt gcgccttggc tcgcgagcca
     3481 ttccccgatt gcgagggtgc ggtcagcgat gaagtcgtcg tcgcgttcgc cgtagggggc
     3541 gtagcgcgag tcggcgacgt agatgagcgc ttcgtgaggc agttgcgccc ggaccgcccg
     3601 cagcaccgac aaaccaccca gacccgagtc gaagatgccg accggcgccc gcgaatcgcc
     3661 acctgagggg gcaatcccgg cactggacga cagcgattct gcaggcatag gcaataagga
     3721 cgcgcgtggc acagcggtca cgggaaactc acgaaaacgc ggaagtgctc aagtatatcg
     3781 cccgcccggt tcaggactcg atcgaaccca tcgcagtttg ctgataattc tggataccga
     3841 ccttgtcgat caggtcgatc tgcgtttcga gccagtcgat gtgttcttcg gtgtcgtcga
     3901 gaatcttcac gaagatttca cgcgagatga aatcgcgaac cgattcgcaa tacacgattg
     3961 cttccttgca ggtgctctgc gaaatctgtt cgagcttcag gtcgcattcg aggatctctt
     4021 tggtttcctc gccgatcagc agcttgtgca ggtcttgcag attcggcagg ccgtcgagca
     4081 tgaaaatgcg ttcgatcaac cagtcggcgt gcttcatttc gccgatcgat tcgtcgtatt
     4141 catgtttgcc gagtttctcg agaccccagt gcttgtacat ccgcgcgtgc aggaagtact
     4201 ggttgatggc ggtgagttcg ttcttcagtt gcgagttcag atactcgata acttttttgt
     4261 cgccttgcat gggggttcct tatgggggat ttgaattttc aacaacaata aacgccggac
     4321 ctgaaaaagc caaggaaaca gacacagttt tttgccttga ttcgcaaggt ttttttgcgt
     4381 gatgcgtact gttctcattc caagataaaa agccggagtt tgaggccccg gctttcattt
     4441 ttcagctgat gttgctaccg atcaaactgt cgcgaccgga atcttgccga tcttcgcttg
     4501 ccattccttg ggtccagtct ggtgcaccga ggtgccgttc gaatcgaccg cgacggtgac
     4561 gggcatgtcc tgcacgtcga actcgtagat cgcttccata ccgaggtctt cgaacgcgag
     4621 aaccttcgcg ctgcgaatgg ctttcgacac gagataagcg gcgccgccca cggccatcag
     4681 atacgcggcc ttgtgcttct tgatcgcctc gatcgcaacc gggccgcgct cggccttgcc
     4741 gatcattgaa acaagaccgg tttgcgacag catcgtctca gtgaacttgt ccatgcgcgt
     4801 cgcggtggtc gggccggcgg ggccgactgc ttcgtcgcgc accggatcga ccgggcctac
     4861 gtaatagatc acgcggttcg tgaagtcgac cggcagcttt tcgcccttcg ccagcatatc
     4921 ggcgatgcgc ttatgcgcgg cgtcgcggcc cgtcaacatc ttgcccgaca gcagcagcgt
     4981 ctggcccggc ttccagcccg cgacttctgc cggcgtcagc gtgttcagat cgacgcgctt
     5041 gctcttttca gtgtccggtt cccactgcac cttcggccat gcgtcgagcg atggtgcctc
     5101 gagcttggcc acccccgagc cgtccagcgt gaagtgcgcg tgacgcgttg ccgcgcagtt
     5161 cgggatgatc gcgacc
//
LOCUS       DATASH010000279         1579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1621158,
            whole genome shotgun sequence.
ACCESSION   DATASH010000279 DATASH010000000
VERSION     DATASH010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1621158"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VHV99_07820"
     CDS             complement(<1..227)
                     /locus_tag="VHV99_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635381.1"
                     /translation="MSRGAAAADAETYRPELLGDEEPRYLRRQKPVEIRRKKFSGRGW
                     SFYRRVLFYGVVGAAGASAAWLAGHFLLYAQP"
     gene            complement(233..1141)
                     /gene="murB"
                     /locus_tag="VHV99_07825"
     CDS             complement(233..1141)
                     /gene="murB"
                     /locus_tag="VHV99_07825"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEX3635382.1"
                     /translation="MRIEDAKIVAELPELGAELRRSVSLAELTSLGIGGTTDLLRIMK
                     HESIPGLLRLLDSSGVPHRFLGGGSNLLVGDGELPWVVLQLASPEPDVVLEGNFAQVD
                     AAADLGRMVTYCAKHDLGGMEGLIGVPGTVGGALRMNAGAYGMQIGSYVREVKVYRAA
                     SRRIEILRGEQISFEYRHTSFAADDMMLAVKLELPSKPFKEILQGIRICNEKRRSSQP
                     LGQKSAGCIFKNPPGASAGRMIDELGLKGLSVGDARVSDRHANFFVNAGKASAREMLA
                     LISDVRERVKAKFQVELEHEVVIWNA"
     gene            complement(1131..>1579)
                     /locus_tag="VHV99_07830"
     CDS             complement(1131..>1579)
                     /locus_tag="VHV99_07830"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEX3635383.1"
                     /translation="DITVIDDYGHHPAEIRATLEAARICKFNRLLVLFQPHRFTRTQH
                     LWDEFCRAFNQADVLVLTDIYAASEAPIPGITSEALANAIREAGHKNVHYFRSMQESI
                     EYLLREARPLEAILTIGAGNVSRASNELMVLLGTEYPSDRPIRHAH"
BASE COUNT          305 a          532 c          432 g          310 t
ORIGIN      
        1 ggctgcgcat atagcaggaa atgcccagca agccatgcgg ccgaagcccc cgcggctccc
       61 accacgccat aaaacaaaac gcgccggtaa aacgaccagc cccgcccgct gaatttcttg
      121 cggcgaattt ccaccggttt ctgacgccgc aagtagcgtg gctcttcatc gcccagcagc
      181 tccggccggt acgtctcggc gtccgccgcc gcggcgccgc gcgacatggt cttcatgcgt
      241 tccaaatgac cacctcgtgc tccagttcaa cttggaactt tgccttcacc cgttcgcgca
      301 cgtcggagat cagcgccagc atctctcgcg cactggcttt ccccgcgttt acgaaaaaat
      361 tggcgtgccg gtcgctcacg cgcgcgtcgc cgacgctcag gcctttaagg cccagctcat
      421 caatcatgcg tcccgcggat gcccctggag gatttttgaa gatgcagccg gcgcttttct
      481 ggcccagcgg ctggctcgac cgccgcttct cgttgcagat acgaatccct tgaagtattt
      541 ctttgaacgg tttggaggga agttccagct tcaccgcgag catcatatca tcagccgcaa
      601 acgaagtgtg acggtattcg aaggaaattt gttcgcccct gaggatttct atccgccggc
      661 tggctgcacg gtacaccttg acctcccgga cgtacgaccc gatctgcatc ccgtaagccc
      721 cggcattcat ccgcagcgcg ccccccaccg tgcccggcac tccgatcagc ccctccattc
      781 cgccgagatc gtgcttggcg caataggtga ccatgcgccc caggtccgcc gcggcatcca
      841 cctgcgcgaa attcccttcc agtaccacgt cgggctccgg cgacgccaac tgcagcacca
      901 cccacggaag ttcgccgtcg cctaccagca agttcgaacc gccgcccaga aagcgatgcg
      961 gcacgccgga agaatcaaga agtcttagca ggccagggat ggattcatgt ttcatgatcc
     1021 gcagcagatc ggtagtcccg ccgataccca gcgaggtcag ctcggccagc gacacgctgc
     1081 ggcgcagttc agcacccagc tccggcaact ctgcaacgat tttggcgtct tcaatgcgca
     1141 tgacgaattg ggcggtcgct cggatactcc gtgcccagca gcaccatcag ttcattgctg
     1201 gcgcggctca cgttcccggc cccaatggtc agaatcgctt caagcggccg cgcttcgcgc
     1261 agcaaatact cgatgctttc ctgcatcgac cggaaatagt gcacattctt gtgcccggct
     1321 tcgcgaattg cgttggccaa ggcttcgctg gttatgccgg gaatgggcgc ttcgctcgcc
     1381 gcgtaaatat ccgtgagcac cagcacatcc gcctggttga acgcgcggca gaattcatcc
     1441 cacaaatgct gcgtgcgggt gaagcgatgc ggctggaaca gcaccagcag ccggttgaac
     1501 ttgcagatgc gcgcagcctc cagggtcgca cggatttcgg cgggatggtg gccgtaatcg
     1561 tcgatcactg taatgtcgt
//
LOCUS       DATASH010000280         9211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3400103,
            whole genome shotgun sequence.
ACCESSION   DATASH010000280 DATASH010000000
VERSION     DATASH010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 9211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9211
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3400103"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /gene="lptD"
                     /locus_tag="VHV99_07835"
     CDS             <1..704
                     /gene="lptD"
                     /locus_tag="VHV99_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HEX3635384.1"
                     /translation="DRIADANRLTAALTTRFINPATGDDRARFVIAQQYYFQDQRVTL
                     LPTQTSTQATHSDLILGASLKLGAGFASETAFQYNADNNELMKTSVGFGFSPASGKVI
                     NVAYRYTRANTTLDNQPINQVLISGQWPLTHRVYGVGRFNYDMDGHRIVDGLIGLQYD
                     ADCWTLGAGIQRYANGVNTSAQQQTGTRFLAQLTFKGLSSVDNGLVSAFRAGVAGYTP
                     LPPPPPPEARFTNYE"
     gene            772..2136
                     /locus_tag="VHV99_07840"
     CDS             772..2136
                     /locus_tag="VHV99_07840"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX3635385.1"
                     /translation="MAIMKKLRLATLAAGLVAVASFLSVAPVQAQALSSGNGGQTVDT
                     IAAVVNNGVITRRELDERIGLITRRLNQQNAPVPPMDQLRQQVLNQMVLERIQLQKAK
                     EDGITIDDAAVQKTLERLAQANNMTLEIYRARIEAQGVPWTTFTSDARTELTLSRLRE
                     KEVDSKVTVSDAEVANYIASQRGPNAGLTNDLHLQHILVKAPLNASETDIEAAQQKAQ
                     ALLTEAKGGANFEKLAKSNSQAPDASKGGDTGFLPPSKLPPEFVKAASALRPGEVNPD
                     LIRTNDGFEIVRLVDRRAGQGASSDAPKLVQTHVRHILLRVGDGMSEPQARQRLLDIK
                     SQIAAGGDFAKFAHTYSQDGSSSQGGDLGWISPGETVPEFERAMNNLQDGQISDPVRS
                     EYGYHLIQVLGRRESEGSVSQQMDLARQAIGQRKAEQAYADWLRELRDTAYVEVKPTL
                     TSTQ"
     gene            2142..3152
                     /gene="pdxA"
                     /locus_tag="VHV99_07845"
     CDS             2142..3152
                     /gene="pdxA"
                     /locus_tag="VHV99_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018431931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEX3635386.1"
                     /translation="MTSAAQSTSLPLQIAITTGEPAGVGPELTAQALAGAAAHWPGAQ
                     FTVLGDADLLAERARAVGVDWAALVADGKRVRVQHRPLGAPSLAGKLDAANGRYVLNL
                     LDRAIDGAVAGTFDAIVTAPLQKSTINDAGVPFTGHTEYLAERTHTPRVVMMLAGTGK
                     RPLRVALATTHLPLKDVSAALTVEGIVETLRIIDHDLRHHFGLPAPRILVTGLNPHAG
                     ENGYLGREEIDVIAPALKLANAQGIDAPGPYPADTLFQPRYLEQADCVLAMFHDQGLP
                     VLKYATFGEGINVTLGLPIIRTSVDHGTALDLAGTGRADAGSLIAAIDTAVSMAQHRR
                     VG"
     gene            3443..4309
                     /gene="rsmA"
                     /locus_tag="VHV99_07850"
     CDS             3443..4309
                     /gene="rsmA"
                     /locus_tag="VHV99_07850"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEX3635387.1"
                     /translation="MSTSRQQQGRHQGHIARKRFGQNFLVDMGVIDSIVDVIRPQRGE
                     RMVEIGPGLGALTEPLIERLATPEAPLHAVELDRDLIGRLKTKFGDLLELHAGDALAF
                     DFGTLATPGEKASLRIVGNLPYNISSPLLFHLTAFAHRVIDQHFMLQNEVVDRMVAEP
                     GTKAFSRLSVMLQYRYVIDKQLDVPPEAFNPPPKVDSAIVRMIPYELHELPAVDERVL
                     GEVVTAAFSQRRKMLRNTLAAYRDLVDFEVLGFDLQRRAEDVPVAEYVQVAQIVAASP
                     ARGAAAAGNGET"
     gene            complement(4382..5317)
                     /locus_tag="VHV99_07855"
     CDS             complement(4382..5317)
                     /locus_tag="VHV99_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX3635388.1"
                     /translation="MSTTALPVRRAPDSFAIVLMIGLCAIWGLQQVAIKSTNAALPPV
                     FQAGLRSAIAAVLVWGWARSRGTPLFRDDGTLAAGLLAGVLFAGEFVCIFLGLTLTSA
                     SRMAVFLYTAPCFTALGLHWFVDGERMRRIQWLGILVAFAGMALAFADGFLHGHGAQG
                     ATLKGAAGDALGVLAGIAWAATTVVVRATRLAHSSASKTLFYQLTVSAVVLIALAVGL
                     GQTRVDTVTPLALVSLAYQAVIVAFVSYLVWFWLLTRYIASRLSVFSFLTPLFGVTFG
                     VLLLGESFSLRFLMAAVLVLTGIALVNAPGKPRAR"
     gene            5670..6779
                     /locus_tag="VHV99_07860"
     CDS             5670..6779
                     /locus_tag="VHV99_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX3635389.1"
                     /translation="MKNARRWGIAVVPAALACQMAHAQSSVTLYGVVDESVRYLTHVN
                     SAGDSKLALGNGGTTESRWGIRGVEDLGGGWSTFFKLENRFYINSGQSDPNMPFFNEA
                     QVGVRSDAYGQVILGRQYNVMIEGITIGGYGSNLWIPYDFSFQPEVTMTGGIWTSNQV
                     QYQAKYNGFYFAAGYAFGGNAGNSYGSQVGATLAYAPGGPFTLGAAYENSKDSVNGSA
                     AKDWTFGGSYTWNMTRFSVGYIVNQNDAGFSSFANGPFTAPALAALKYTDFSRRRMIM
                     GGITQQVGTAWHFAANVWRTLQDGKTAAQDGSAWQYQLIADYNLSRRTDVYVEGDYSL
                     YRGDLIGAQLQGVNGVGLAQKGSQIGLMAGLRHQF"
     gene            complement(6784..>9211)
                     /locus_tag="VHV99_07865"
     CDS             complement(6784..>9211)
                     /locus_tag="VHV99_07865"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015474 - autotransporter activity
                     [Evidence IEA];
                     GO_process: GO:0046819 - protein secretion by the type V
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HEX3635390.1"
                     /translation="GGTGNNVLTNHGTIDVSGVNAHGLVSLDTVPGVVTNTGSVTAHG
                     SGGLGAFFSGNITLDNLSGASIVSQQANGIVANGGGIFNNAGTISSAGNAIAIANGAA
                     TVTNSGSLTSTSSEAISATGTFDIAINNTGMIAGGNGAAIWTNGGNDTFNWSGGTVIG
                     FVRLDAGNDTANLTGLTDANLAGVPSLDGGTGNDVLNFNRTSASGLGRFVNWETINVT
                     NGSQLTLDANGLVLGDSSTLTGSLNIDASSTVFAGGFGGTAAIAPAVAGQRVNVNNAG
                     TLDLTNGATATRNTLVINGNYTGLNGRLLLQTVLGADGSPSDKLVIAQGVGSGNTSLG
                     VTNVGGTGGATLTDGILVVQAANGATTTANAFTLPRPLSVGAYTYYLFKGGVSAGTAD
                     NWYLRSSVAAAPAPTPTSTPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTP
                     TPTPTPASTPTPTPVASAAPIPAPGTPALPAPPSAGAAPTPLYRMEVPVYAEVPVLTR
                     ELGIAQIGTFHDRQGEQALLNDSGALAATWARVWGEHSSQSNSGAANPEFSGTMTGVQ
                     VGQDIYADRSSTGHRNHYGFFIGFARAQGDVNGFALGFPDLSAGRLSINAYSAGVYWT
                     HIGPGGWYTDAVAMGSSLTLDPSSNQGIGASTHGHSVTTSLEAGLPIPLRTNLSLEPQ
                     AQLIWQHASINDLNDGISNVSFHAANGLVGRLGLRLQGSFEGAGAQWQPYLRANLWRY
                     FNGTDSATFAGTTVIPTDVAATAAQFGIGMVARLGARGSVFITASYTTNVNGEHRSTV
                     EGNLGARWSW"
BASE COUNT         1680 a         2886 c         3018 g         1627 t
ORIGIN      
        1 atgaccggat cgccgacgcg aaccgtctga cggcggccct taccacgcgc ttcatcaacc
       61 cggccaccgg cgacgatcgc gcgcgtttcg tgatcgcgca gcagtactac tttcaggacc
      121 agcgcgtcac gctgctgccg acccagacca gcacgcaggc cacgcattcg gatctgatcc
      181 tgggcgcgtc gctcaagctc ggcgccggtt tcgcttcgga aaccgcgttc caatataatg
      241 ccgacaacaa cgagttgatg aagacgagcg tgggcttcgg gttcagtccg gccagcggca
      301 aggtgatcaa tgtcgcgtat cgctacaccc gcgcgaatac cacgctggac aatcagccaa
      361 tcaatcaggt gctgatctcg gggcaatggc cgctgacaca ccgggtgtac ggggtaggcc
      421 gtttcaacta cgacatggac gggcatcgga tcgtcgatgg tctgatcggc ctgcaatacg
      481 acgccgactg ctggaccctc ggcgccggga ttcagcgcta tgcgaacggg gtgaacactt
      541 cggcccagca gcagacgggc acgcggtttc tcgcgcagtt gacgttcaag ggcttgtcga
      601 gcgtcgacaa cgggctggtt tccgccttcc gcgccggcgt ggcgggctat acgccattgc
      661 cgccaccgcc gccgccggaa gcgcgtttca ccaattacga atgacgctcg cacgctcatt
      721 catagaatgc gaaaagacgg gccaggcgcg cccgacatca ttggagtatc tgtggcaatc
      781 atgaaaaagc ttcgcttggc aacgcttgcg gccggtcttg tcgccgtggc gtctttcctg
      841 tcggttgcgc cggttcaggc gcaggcgctg tcgtccggca atggcggcca gacggtcgac
      901 accattgccg cagtggtcaa caacggtgtc atcacgcggc gcgagctcga cgagcgcatc
      961 ggcctgatca cgcgccggtt gaaccagcag aacgcgccgg tcccgccgat ggaccagttg
     1021 cgtcagcagg tgctcaacca gatggtgctg gaacgcatcc agttgcagaa ggcgaaagaa
     1081 gacggcatca ccatcgacga cgccgccgtg caaaaaaccc tcgagcggct cgcgcaggcg
     1141 aacaacatga cgctcgagat ttaccgcgcg cgtatcgagg cgcaaggggt gccctggacc
     1201 accttcacga gcgacgcgcg caccgagctc acgctctcgc gtctgcgtga gaaggaagtg
     1261 gacagcaagg tcaccgtgtc ggacgccgag gtcgcgaact atattgccag ccagcgtggc
     1321 ccgaatgccg gcctgacgaa cgatctgcac ctgcagcaca ttttggtgaa ggcgccgttg
     1381 aacgcttcgg aaaccgacat cgaagccgcg cagcaaaaag ctcaggccct gctgaccgaa
     1441 gccaagggcg gcgccaattt cgaaaagctg gcgaagtcga attcgcaggc accggatgcg
     1501 tccaaaggcg gcgatacggg cttcctgccg ccgtccaagc tgccgcctga gttcgtcaag
     1561 gctgcttcgg cgctgcgccc gggcgaggtc aatccggatc tgatccgcac caacgacggc
     1621 ttcgaaatcg tgcgcctcgt cgatcgtcgc gcgggccagg gcgccagttc cgatgcaccg
     1681 aagctcgtgc agacgcacgt tcgccacatt ctgctgcgtg tcggcgacgg catgtccgag
     1741 ccgcaagcgc gtcagagact gctcgacatc aagagccaga tcgccgcggg cggcgacttt
     1801 gcgaagttcg cgcacaccta ctcgcaagac ggttcgtcgt cgcaaggcgg tgacctcggc
     1861 tggatcagcc cgggcgagac ggtgccggaa ttcgagcgcg ccatgaacaa cctgcaggac
     1921 ggtcagatca gcgacccggt gcgcagcgaa tacggctatc acctgattca ggtgctgggc
     1981 cgccgcgaat cggaaggctc ggtttcgcag cagatggatc tggcgcgtca ggcgatcggt
     2041 cagcgcaagg cggaacaggc ctacgccgac tggctgcgcg aattgcgcga caccgcctac
     2101 gtggaagtga agcccacgct gaccagcacg caataaccat catgacaagc gccgcgcagt
     2161 ccaccagcct tccgttgcag atcgcgatca cgaccggcga gcctgccggc gtcggccccg
     2221 agctgaccgc gcaggcgctg gcgggcgcgg cggcgcattg gcccggcgcg cagttcaccg
     2281 tgctgggcga tgcggatctg ctggcggaac gcgcacgcgc ggtcggcgtc gattgggctg
     2341 cgctggtggc cgacggcaag cgcgtgcggg tacagcatcg gccgctcggc gcgccctcgc
     2401 tggctggcaa gctggacgcc gccaacggcc gctacgtgct caatctgctc gacagagcga
     2461 tcgacggcgc cgtggccggc acgttcgacg cgatcgttac cgcgccgctg caaaaaagca
     2521 ccatcaacga tgccggcgta ccgttcaccg gccacacgga atatctcgcc gagcgcacgc
     2581 acacaccgcg cgtggtgatg atgctggccg gcaccggcaa acgtccgttg cgcgttgcac
     2641 ttgctaccac gcatctgccg ctcaaagacg tctccgccgc gctgacggtc gaagggatcg
     2701 tcgagacgtt gcgcatcatc gatcacgatc tgcgccacca ttttggtttg cccgcgccgc
     2761 gcattctcgt cacgggcttg aacccgcatg cgggcgaaaa cggttatctg ggccgcgagg
     2821 agatcgacgt gattgcaccg gcgctgaagc tcgcgaacgc gcaaggcatc gacgcgcccg
     2881 gcccctatcc cgccgacact ttgttccagc cgcgttatct ggaacaggcc gactgcgtgc
     2941 tggccatgtt ccacgaccag ggcttgccgg ttctgaaata tgcaacgttc ggcgaaggca
     3001 tcaacgtcac gctcggtttg ccgatcatcc gcacctcggt cgatcatggc accgcgctcg
     3061 atctggccgg cacaggccgt gccgacgcgg gcagtctgat cgccgcgatc gatacggcgg
     3121 tgtcgatggc gcagcaccgc cgcgtgggct gatagtgccg ggtcgatcgc gtagcgccgc
     3181 cgcgcgggtt gctactgccg tgtcgatggc accgccgcgc ggggttgcta ctgccgtgcc
     3241 gatggcaccg ccgcgcgggc tgatacggcc gtgtcgatgg cctatcaccg ccgcgcgggc
     3301 tgatccagcg gcctcgatgg cgtagcattg ccaggcaggc cgcaacagcc gccaaggcgt
     3361 acggcctcat cagcgtttcc cacggtcgcg agttgttccg cttgctcatt tttaagcagt
     3421 tctctttctt agcgtttctt cgatgtccac cagcagacag caacagggcc ggcaccaagg
     3481 ccatatcgcg cgcaagcgtt tcggccagaa ttttctggtc gatatgggcg tgatcgattc
     3541 gatcgtcgac gtgatccggc cgcagcgcgg cgagcgcatg gtcgagatcg ggccgggcct
     3601 cggcgcgctc accgaaccgc tgatcgagcg tctggcgacg cccgaggcgc cgttgcacgc
     3661 ggtggaactg gatcgcgatc tgatcgggcg cctcaagaca aaattcggcg acctgctcga
     3721 gttgcacgcg ggcgatgcgc tcgcgttcga cttcggcacg ctggcgacgc ctggcgagaa
     3781 ggcgtcgctg cgcattgtcg gcaatctgcc gtacaacatt tcgagcccat tgctgtttca
     3841 tctgaccgcg ttcgcgcatc gcgtgatcga tcagcacttc atgctgcaga acgaagttgt
     3901 cgaccgcatg gtggccgagc cgggcaccaa ggcgttcagc cgcctctcgg tgatgctgca
     3961 ataccgctac gtgatcgaca agcaactcga cgtgccgccc gaggcattca acccgccgcc
     4021 gaaggtcgat tcggcgatcg tgcggatgat tccctacgag ctgcatgaat tgccggccgt
     4081 ggacgagcgc gtgctcggcg aagtggtgac ggcggccttc tcgcagcggc gcaagatgct
     4141 gcgcaacacg ttggccgcgt atcgtgactt ggtggatttc gaggtgctgg gcttcgatct
     4201 gcaacgccgc gccgaggatg tgccggtagc cgaatatgtg caagtggcgc agatcgtagc
     4261 ggcttcgccg gcgcgtggcg ccgcggctgc cggaaacggc gagacctgaa aggcgcagca
     4321 caagcttgaa aagaggcgcg ccaccatccg gggtggcatg cctggacatc ccaccacgtt
     4381 cctaacgcgc ccgaggtttc ccgggcgcat tgaccagcgc gatgccggtc agcaccagca
     4441 cggccgccat cagaaagcgc aggctgaacg attcgccgag cagcaatacg ccgaaggtca
     4501 cgccgaacaa cggcgtcaag aacgagaaca ccgacaggcg cgacgcgatg tagcgcgtca
     4561 aaagccagaa ccacaccaga tagctgacga acgccacgat cacggcctga tacgcgaggc
     4621 tcaccagcgc cagcggcgtg acggtgtcca cgcgtgtctg gcctagcccc actgcgagcg
     4681 cgatcagcac caccgccgac accgtcaatt gatagaagag cgtcttgctg gcgctcgaat
     4741 gggcgaggcg cgtggcgcgc accaccacgg tggtcgcggc ccacgcgatg ccggccagca
     4801 ccccgagcgc gtcgcctgcg gcgcccttca gcgtcgcgcc ctgcgccccg tggccgtgca
     4861 ggaagccgtc cgcgaatgcc agcgccatac cggcaaacgc caccaggatg ccgagccact
     4921 gaatgcgccg catccgctcg ccgtcgacga accagtgcag cccgagcgcc gtgaagcagg
     4981 gcgcggtgta cagaaagacc gccatgcgcg aagcgctggt cagcgtcagg ccgagaaaaa
     5041 tgcagacaaa ctcccccgcg aacagcacac ccgcgagcaa gcctgcagcc aacgtgccgt
     5101 cgtcgcgaaa gagcggggtg ccgcgcgagc gcgcccagcc ccataccagc accgccgcga
     5161 ttgctgagcg caagccggcc tggaataccg gcggcagcgc cgcgttggtg ctcttgatcg
     5221 caacttgctg gagtccccag atcgcgcaca agccgatcat cagcacaatg gcaaagctat
     5281 ctggcgcgcg gcgcacgggc agggcagtgg tgctcatgag gggtgtcgag tggcgatcat
     5341 aggtcatacg atatcaaaac cgttcgagcc gcaatgcgat gaccccaccg cctacgcgtg
     5401 cttcttacaa gctgcaagca cttcgatgac ttaacaacac ttacatcagt ctattttctc
     5461 gcggcttcag attcgcgcta aggttcactc aaacgcattt tgtcagctta ttcgcgcagc
     5521 cggcagggcc gtcatacaag gctccgaggc ggactctgct ccgtatctgc cgatttgtgc
     5581 ggtccagatg agcagcgtgc cggtactttg catcaaacgc ggcggtgtcg aaatatccgc
     5641 gaaaggcggg aatctgtaca ggaaacgaga tgaagaacgc aaggcggtgg gggatcgcag
     5701 tggtgcccgc agcgttggca tgtcagatgg cgcacgcgca atccagcgtg acgctctacg
     5761 gtgtagtcga cgaaagcgtg cggtatctca cccacgtaaa cagcgccgga gattcgaagc
     5821 tcgcgctcgg caacggcggc acgactgaaa gccgctgggg cataagaggc gtcgaagacc
     5881 tgggcggcgg ctggtctact ttcttcaagc tcgaaaaccg tttctacata aatagcggtc
     5941 aaagcgatcc caacatgccg ttcttcaatg aggcgcaagt gggcgtgcgc tcagacgcgt
     6001 acggtcaggt gatcctcggc cgtcagtaca acgtgatgat cgagggcatc accatcggtg
     6061 gttacggcag caatctctgg attccgtacg atttcagctt ccagccggaa gtgaccatga
     6121 ccggtggtat ctggaccagc aatcaggtgc agtaccaggc gaaatataac ggcttctatt
     6181 ttgccgctgg ttatgcgttc ggcggcaacg cgggcaattc gtacggcagc caggtcggcg
     6241 cgacgcttgc ctacgcaccg ggcggaccgt tcacgctcgg cgccgcgtat gaaaattcga
     6301 aagattcggt caacggctca gccgcgaagg actggacctt cggtggatcg tacacgtgga
     6361 acatgacccg gttctccgtc ggctatatcg tcaatcagaa cgacgccggc ttttcgagct
     6421 tcgccaatgg gccgttcacc gcgcccgcgt tggccgcgct caagtacacc gacttttcgc
     6481 ggcgccgcat gatcatgggc ggcatcacgc agcaggtcgg cactgcgtgg cacttcgccg
     6541 cgaacgtctg gcgcacgctg caggacggca agaccgccgc gcaggacggt tcggcatggc
     6601 agtaccagct gatcgccgac tacaacctct cgaggcgcac cgatgtctat gttgaaggcg
     6661 attactcgct ataccggggc gacctgatcg gcgcgcagtt gcagggcgtc aacggggtcg
     6721 ggctcgcgca gaagggcagc cagatcggcc tgatggccgg tctgcggcat cagttctgag
     6781 cccttaccaa ctccagcgcg cgcccagatt tccctccacc gtgctgcggt gctcgccgtt
     6841 cacgttcgtg gtgtaactcg ccgtgatgaa cacgctgccg cgcgcgccca aacgcgcgac
     6901 catgccgatg ccgaactggg cggccgtggc ggcgacatcg gtgggaatca cggtcgtgcc
     6961 ggcgaaggtc gcgctatcgg tgccgttgaa atagcgccac agattcgcgc gcagatacgg
     7021 ttgccattgc gcgcccgcgc cttcgaagct gccttgcagg cgcaggccga gccggcccac
     7081 caggccgttt gccgcgtgaa acgatacgtt cgaaatgccg tcgttcagat cgttgatcga
     7141 tgcgtgctgc cagatcagtt gcgcttgtgg ttcgagactg agattggtgc gtagcggaat
     7201 cggcaggccg gcttccagtg aggtggtgac cgagtggccg tgcgtactcg cgccaatgcc
     7261 ctgattcgac gaaggatcga gcgtgagtga gctgcccatc gcaaccgcat cggtgtacca
     7321 gccgcctggg ccgatatggg tccaataaac gcctgcactg tatgcattga tcgacagccg
     7381 cccggctgag agatcgggga agccgagcgc gaagccgttc acgtcgcctt gggcgcgggc
     7441 aaagccgatg aagaagccat agtgattgcg atggccggtt gagctgcgat ccgcgtagat
     7501 gtcctgaccc acttgcacgc cggtcatcgt gccgctgaat tcggggttgg cagcgccgct
     7561 gttgctttgc gagctgtgct cgccccagac acgggcccat gtggcggcaa gcgcgccgct
     7621 gtcgttcagc agcgcctgct cgccctggcg gtcgtggaag gtgccgatct gcgcgatacc
     7681 gagttcgcgc gtgagaaccg ggacctcggc atagacgggc acttccatgc ggtaaagcgg
     7741 cgttggggct gcgcccgcgg acgggggggc gggcagggcc ggcgtgccgg gtgcagggat
     7801 cggagcggct gaggcgacgg gtgtcggtgt gggcgttgat gccggtgtag gcgttggtgt
     7861 tggcgttgga gtaggtgtag gtgttggtgt tggtgttggt gttggtgttg gtgttggtgt
     7921 tggtgttggc gttggtgttg gcgttggagt aggtgtgggc gttggtgttg gtgttggtgt
     7981 tgatgtcgga gtcggagccg gagccgcggc cacactcgag cgcaaatacc agttgtctgc
     8041 cgtccccgcg ctcacaccgc ccttgaacag atagtacgta taagcgccca cggacagcgg
     8101 cctcggcagc gtgaaggcgt tcgccgtcgt ggtcgcgccg ttcgccgcct gcacgacgag
     8161 aatgccgtcc gtgagcgtgg cgccgcccgt gccgccgaca ttggtgacgc caagcgaagt
     8221 attgcccgag ccgacgcctt gcgcgatcac cagcttgtcg ctcggcgagc cgtctgcgcc
     8281 cagcaccgtc tgtagcagca agcggccgtt gagcccggtg tagttgccgt tgatcaccag
     8341 cgtattgcgc gtcgccgtcg cgccgttggt cagatcgagg gtgcccgcat tgttgacgtt
     8401 cacgcgttga cccgcgactg ctggcgcaat cgccgccgtg ccgccaaagc cgcccgcaaa
     8461 caccgtgctg ctcgcatcga tgttgagcga gccggtcagt gtgctgctgt cgccgagcac
     8521 gaggccgttg gcgtcgagcg tcaactggct gccgttggtg acgttgatgg tctcccagtt
     8581 gacgaagcgg ccgaggccgc tcgccgacgt ccggttgaaa ttgagcacgt cgttgccggt
     8641 gccaccgtcg aggctcggca cgccggcgag attggcgtcg gtgaggccgg tgagattggc
     8701 ggtgtcgttg cctgcatcga gacgcacgaa gccgatgaca gtgccgccgc tccagttgaa
     8761 ggtgtcgttg ccgccgttgg tccagatcgc ggcgccgttg ccaccggcga tcatgccagt
     8821 gttgttgatg gcgatgtcga atgtgccggt ggcgctgatc gcctcgctcg acgtcgaagt
     8881 gagcgagccg ctgttcgtca cggtcgccgc gccgttggcg atggcgattg cgttgccggc
     8941 gctcgaaatt gtgcctgcgt tgttgaagat gccgccgccg ttcgcgacga tgccgttggc
     9001 ctgctggctg acgatgctgg cacccgacag attgtctagc gtgatgttgc cggaaaagaa
     9061 tgcgccgagg ccgccggatc cgtgcgcggt gaccgagccg gtattcgtca cgacgccggg
     9121 cacggtgtcc aacgaaacga ggccgtgtgc attcacaccc gagacgtcga tcgtgccgtg
     9181 attcgtcagc acgttgttgc cggttccgcc g
//
LOCUS       DATASH010000281         2968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4983530,
            whole genome shotgun sequence.
ACCESSION   DATASH010000281 DATASH010000000
VERSION     DATASH010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4983530"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1295
                     /locus_tag="VHV99_07870"
     CDS             <1..1295
                     /locus_tag="VHV99_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX3635391.1"
                     /translation="TSTTLTTLSRVQIDPATMPLPELAAHRFDPSDGLDIEEIAMLAV
                     ANNPDLKLARDDLGIARAQAFSAGLLPDPQLAISSDYPGMEGATRAFNYGLTMDVLAI
                     FTRGPNKQSADATVAKTDLGLLWQEWQVVAQAKQLFIRTRFQEDTLPLLTQQRDLSRT
                     RYERMADAQREGNLTEDTLTAALTSYSDARKLYTDAERAAEQTHHDLNQLLGLSPNVK
                     LQLVGESDDASLDENAVHDALAALPRRRPDLLALQAGYEAQEQKYRAAIMSQFPSLTV
                     GFDRQRDTSNIYTSGFQINLSLPIFNRNRGNIAIEQSTRQRLRDEYQSRLNQAFADVA
                     HLRADDAILSRQLEQTEAALPGTEHAARDASTAFAEHNLTLGAYTDAMSAALTKRVDI
                     ATLRESRAEQRVGLQALLGSAIPDAFSSDLNLTEAHAK"
     gene            1381..2367
                     /locus_tag="VHV99_07875"
     CDS             1381..2367
                     /locus_tag="VHV99_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018417798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX3635392.1"
                     /translation="MVAVTTASVQRIQIAQPVRAYGVVAASAANLTSINLPYVARITQ
                     LRVQTGQPVKRGDPLFVVQADPAAALAATQARSAATLADGELARTQSLFDKGLATASQ
                     LAAARKAAQDAREMLVSQAQSGITSGAKVVASPIDGVVLQISAGHGDQVQAGAPIMQL
                     AGADRGQQARGNVILAVEPSDAINIHAGDEVRLRGLSTSLAKVSMTGRVVLVGASIDP
                     QSQLVDVGANVPLGNSDFIPGTHVAADIDTNRGMHWVVPRSAVLKDDHGDFVFQVSSA
                     NKAHRVNVVTQIEDRDRYGIDGPLSATQPVVVSGNYELKDGMTVQGAGGAPR"
     gene            2364..>2968
                     /locus_tag="VHV99_07880"
     CDS             2364..>2968
                     /locus_tag="VHV99_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003275190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX3635393.1"
                     /translation="MNFSQWMQAHRRSLLFVIGLLAVAGALTAFRLPISLFPNVAFPR
                     AVVSLDAGDRPAEQMATLVTMPVEEALRRVPNVLDVESKTSRGSAEISVNFNWGTDMA
                     QATLQAQSSISEILGTLPQGTSMQVRRMDPTVFPVLAYSLTSKQQSLSALHDLAQFQM
                     RPLLSSVEGVARVDVTGGATDEFEVAVDPARLAAYKLSLADV"
BASE COUNT          561 a          954 c          927 g          526 t
ORIGIN      
        1 atacatcgac cacgctcact acgctgtcgc gtgtgcaaat cgacccggcg accatgccct
       61 tgcccgagct cgccgcgcat agattcgacc cctcggacgg cctcgacatt gaagagatcg
      121 ccatgctggc cgtggcgaac aatcccgatc tgaagctcgc ccgcgacgac ttgggcattg
      181 ctcgggcaca agcattttca gcgggattat tgcccgatcc gcagctggcc atttccagcg
      241 attacccggg catggagggt gctacgcgtg cgttcaatta cggcttgacc atggacgtgc
      301 tcgcgatctt cacgcgtggc cccaacaagc aatcggcgga cgccacagtc gcgaagactg
      361 atctcggcct gctctggcag gagtggcagg tcgtggcgca agcaaaacaa ttgttcatca
      421 ggacgcgctt tcaagaggac acgctgccgc tgcttacgca acaacgcgac ctgtcgcgca
      481 cgcgttatga acgcatggcc gatgcccaac gcgaaggcaa cctgaccgaa gacacgctga
      541 ccgccgcgct gacttcgtat agcgacgcgc gcaagctgta taccgacgct gaacgcgcgg
      601 ccgagcagac gcatcatgac ctgaaccagt tgctgggttt gtcgcccaac gtgaaattac
      661 agcttgtggg cgagtccgac gatgcgtcac tcgacgaaaa cgcggtgcat gacgcgctcg
      721 ccgcgctgcc gcgccggcgg cccgatttgc tggccctgca agcgggctac gaggcgcagg
      781 agcaaaagta tcgcgcagca atcatgagcc agtttccgtc gctgacggtg gggttcgatc
      841 gtcaacgcga cacatcgaat atctacacgt ccggcttcca gatcaacctg agcttgccca
      901 tcttcaaccg caaccgcggc aatatcgcga tcgagcaaag cacgcgccag cgtctgcgcg
      961 atgaatatca aagccgcctc aaccaggcat ttgccgatgt ggcccacctg cgcgccgacg
     1021 atgcgattct ctcgcgccag cttgagcaga ccgaagcggc gttgcccgga acagagcatg
     1081 cggcgcgcga cgccagcaca gcgtttgccg agcacaacct gaccctcggc gcgtacacgg
     1141 atgccatgtc ggccgcgctg accaaacgcg tggatatcgc gacgcttcgt gaatcgcggg
     1201 ccgaacagcg cgttggcttg caagcgctgc tgggcagtgc gatccccgac gcattttctt
     1261 ccgacctgaa cctgaccgaa gcccatgcta aataaccggc tttcgatgcc tgcttgcatc
     1321 gccctgtgtt tttgcctcag cgttttcttc gaagcaaaca acgcccttgc cgatgacagc
     1381 gtggtcgccg tcacgaccgc ttcggtccag cgcatacaga tcgcgcaacc ggtgcgcgcg
     1441 tacggcgtgg tcgcggcatc ggcggctaac ctcacgtcga tcaatctgcc ctacgtcgcg
     1501 cggatcacgc aactgcgcgt gcagacgggt caaccggtca agcgtggcga tccgctgttt
     1561 gtcgtgcaag ccgatcccgc tgctgcgcta gccgccacgc aggcaagaag tgccgcgacg
     1621 ctggccgacg gcgaactcgc gcgtactcag tcgctgttcg acaagggcct cgccaccgcc
     1681 tctcaactcg ctgccgcgcg caaggcggcg caggatgccc gcgagatgct ggtgtcgcag
     1741 gcacagagcg ggatcacgag cggcgccaag gtcgtggcgt cgcccatcga cggcgtggtg
     1801 ttgcagatat cggctgggca tggcgaccag gttcaggcag gtgcgccgat catgcagctt
     1861 gccggcgcgg acaggggcca acaggcgcgc ggcaacgtga tactggcggt cgaaccctcg
     1921 gatgccatca acatccatgc cggcgatgag gtccgcttgc gcggtctgtc gacatcgctc
     1981 gcgaaggtct ccatgacagg ccgcgtggtc ctggtgggcg catccatcga tccgcaaagc
     2041 cagcttgtcg atgtgggtgc gaacgtgccg ctcggcaaca gcgacttcat tccgggcacg
     2101 cacgtggccg ccgatatcga tacaaaccgc ggcatgcact gggtcgtccc gcgttcggcc
     2161 gtgctcaagg acgatcacgg agatttcgtg ttccaggttt cgtccgccaa caaggcgcat
     2221 cgcgtcaacg tggtgacgca gatagaggac cgggaccgct acggcatcga cggaccgctc
     2281 agtgcgacgc aaccggtggt ggtcagcggc aactatgagt tgaaggacgg catgacggtg
     2341 cagggcgcgg ggggggcgcc acgatgaact tcagccaatg gatgcaggct caccggcgct
     2401 cgttgctgtt cgtcatcggc ttgctcgcag tggccggtgc actgaccgcg ttcaggttgc
     2461 cgatatcgct gttccctaac gtggcgttcc cgcgcgccgt tgtctcgctc gatgccggcg
     2521 accgtcccgc cgagcagatg gctacgctcg tcaccatgcc ggtggaagag gcgctgcggc
     2581 gcgtgcccaa cgtactcgat gtcgagtcga agacaagccg cgggtctgca gagatatcgg
     2641 tgaacttcaa ctggggcacg gacatggcgc aggccacgct gcaggcgcaa tcgtcgatca
     2701 gcgagatcct cgggacgcta ccgcaaggca cgtcaatgca agtccgccgc atggatccga
     2761 cggtgttccc ggtgcttgcc tatagcctga cgtcgaagca gcaatcgctt tccgcgcttc
     2821 acgatctggc gcagttccag atgcggccgt tgttgtcatc ggtggaaggg gtggcgcgcg
     2881 ttgacgtgac aggtggcgcc accgacgagt tcgaagtggc cgtggacccc gcacgcctgg
     2941 ccgcctacaa gctctcgctc gccgatgt
//
LOCUS       DATASH010000282         3583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2214789,
            whole genome shotgun sequence.
ACCESSION   DATASH010000282 DATASH010000000
VERSION     DATASH010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2214789"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1385
                     /gene="yidC"
                     /locus_tag="VHV99_07885"
     CDS             <1..1385
                     /gene="yidC"
                     /locus_tag="VHV99_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011850880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEX3635394.1"
                     /translation="RGGTVSRLSLVKQGEDGKTPDLVITLFDRTNNHTYLARTGLLGG
                     DFPNHNDIFTLMPNQQTQLTGDEKSFQLSFESPEKGGVKVIKTYTFTRGSYVIGVDTK
                     IENVGTAPVTPSVYMELVRDDQPVETPRFSHTFIGPAVYTDQHHFQKMTFGDIDKNKE
                     DFVNSADNGWIAMVQHYFASAWIPQQGVKRDIYVEKIDPTLYRVGVKQPVATIAPGQS
                     VDVSARLFAGPEEERMLEGIAPGLELVKDYGWVTIIAKPLFWLLEKIHSYVGNWGWSI
                     VLLTLLIKAVFFPLSAASYKSMARMKAITPRMQALRERFKGDPQKMNAALMELYKTEK
                     VNPFGGCLPVVIQIPVFISLYWVLLSSVEMRGAPWILWIHDLSQQDPFFILPVLMAVS
                     MFLQTKLNPTPPDPVQAKMMMFMPIAFSVMFFFFPAGLVLYYVVNNVLSIAQQYYITR
                     MMGQSKAKAA"
     gene            complement(1490..1963)
                     /locus_tag="VHV99_07890"
     CDS             complement(1490..1963)
                     /locus_tag="VHV99_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEX3635395.1"
                     /translation="MPTSKEKAAFAKRLKDSLPKDIKGGTDLAREFNLLYSSGPAVSP
                     QTAHKWLAGTTIPKRDKLQVLAAWLQKDVHWLHYGPAPSGKAKPLARGEKYPLTPETV
                     EMVTQMANRFAGLSPRQRNLVEELITEFDGGTQQESDAAPEDASASKDKGPSAEA"
     gene            2310..>3583
                     /gene="mnmE"
                     /locus_tag="VHV99_07895"
     CDS             2310..>3583
                     /gene="mnmE"
                     /locus_tag="VHV99_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041491300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEX3635396.1"
                     /translation="MLATDSDPIVAIATAPGRGGIGVVRISFGRAGEAAAQPLMQTLT
                     GQALAPRHASYVPFLDDAGNALDRGIALYFPAPHSYTGEHVLELQGHGGPVVLQLVLQ
                     RCIEAGRAFGLRLAEPGEFTRRAFLNDKLDLAQAEAVADLFEASTEAAARSAGRSLDG
                     AFSRDIHALVEEVITLRMLVEATLDFPEEEIDFLEAADARGKLARIRERLAHVLSEAR
                     QGALLREGLSVVLAGQPNVGKSSLLNALAGAELAIVTPIAGTTRDKVAQTIQIEGIPL
                     HVIDTAGLRDTEDEVEKIGIARTWNEIERADVVLHLLDARTGMNVEDETIAARFPSGV
                     PVVRVLNKTDLTELPPTVTPLDADLDLSEVRLSAKQGDGVALLREELLRIAGWQAGAE
                     SVYLARERHLIALRAAEEHLATAAAHADQNAQA"
BASE COUNT          665 a         1067 c         1113 g          738 t
ORIGIN      
        1 cccgtggcgg cacagtgtcg cggctctcgc tcgtcaagca gggcgaagac ggcaagacgc
       61 ccgatctggt gatcacgctg ttcgaccgca cgaacaatca cacgtatctg gcccgcacgg
      121 gtctgctcgg cggcgatttc ccgaaccaca acgacatctt cacgctgatg ccgaatcagc
      181 agactcaact gacgggcgac gaaaaatcgt tccagctcag cttcgagtcg cccgaaaagg
      241 gtggtgtgaa ggtcatcaag acctacacgt tcacgcgcgg cagctatgtg atcggcgtcg
      301 acacgaagat cgagaacgtc ggcacggcgc cggtgacccc gtcggtctat atggaactgg
      361 tgcgcgacga ccagccggtg gaaacgccgc gcttctcgca cacgttcatc ggaccggctg
      421 tctacaccga ccagcaccac ttccagaaga tgacgttcgg cgacatcgac aagaacaagg
      481 aagacttcgt caattcggcc gacaacggct ggatcgcgat ggtgcagcat tacttcgcgt
      541 cggcatggat tccgcagcaa ggcgtgaagc gcgacatcta cgtcgagaag atcgatccga
      601 cgctgtatcg cgtcggcgtg aagcaaccgg tggcgaccat cgccccgggc caatcggttg
      661 acgtgtccgc gcgtctgttc gccggtccgg aagaagagcg catgctcgaa ggcatcgccc
      721 cgggtctgga actggtgaag gactacggct gggtcacgat catcgccaag ccgctgttct
      781 ggctgctcga gaagatccac agctatgtcg gcaactgggg ctggtcgatc gtgctgctca
      841 cgctgctgat caaggccgtg ttcttcccgc tgtcggccgc gagctacaag tcgatggcgc
      901 gcatgaaggc gatcacgccg cgtatgcaag cgctgcgcga acgcttcaag ggcgatccgc
      961 agaagatgaa cgccgcgctg atggagctgt acaagaccga gaaggtcaat ccgttcggcg
     1021 gctgtctgcc ggtcgtgatt cagattccgg tgttcatctc gctgtactgg gtgctgctgt
     1081 cgtcggttga aatgcgcggc gcgccgtgga ttctctggat tcacgatctg tcgcaacagg
     1141 atccgttctt catcctgccg gtgctgatgg ccgtctcgat gttcctgcag accaagctga
     1201 acccgacgcc gccggacccg gttcaagcca agatgatgat gttcatgccg atcgcgttct
     1261 cggtcatgtt cttcttcttc ccggccggtc tggtgctgta ctacgtggtg aacaacgtgc
     1321 tgtcgatcgc ccagcagtac tacatcacgc ggatgatggg tcagtcgaag gcgaaagcgg
     1381 cctgattgat gtcttgcctg cagcgggcgg cggtagcgcc cgctggctaa ggggtagacg
     1441 ctgaagctag cggtaaaaaa gccgcccggt ttgcaccggg cggctttttt tatgcctcgg
     1501 cggatggtcc cttatctttc gatgccgatg cgtcttcagg tgcggcgtcg ctttcctgtt
     1561 gcgtgccgcc gtcaaattca gtgatcagtt cttccaccag attgcgctgg cgcggcgaca
     1621 aacccgcgaa ccggttcgcc atctgcgtca ccatctcgac cgtttccggc gtaagtggat
     1681 acttttcgcc acgggcaagc ggttttgcct tgccggatgg cgctggcccg taatgcagcc
     1741 agtggacgtc cttttgcaac caggcggcta aaacctgcaa cttgtcgcgc tttggtatcg
     1801 tcgtacccgc gagccatttg tgcgcggttt gtggtgagac cgcagggcca ctgctataaa
     1861 gcagattgaa ttccctggct aaatcggtcc cgcccttaat gtctttgggg agtgagtctt
     1921 tcagccgttt ggcgaaagct gctttttctt tggaggtcgg catgggcccg attgtgcaat
     1981 tcggcgcata atcagtggac aaccagttag gctattttat agcgtaaata agctactttt
     2041 agcctgaaat agccgctgaa taccgtgttc cacgatttct caaaatcctg tcttttcctt
     2101 gtggggtgtg cgttttagcg actctggcgc gtcgattagt cgcaagtgaa tcttaggcta
     2161 tcttagcttg tgcgagataa atccctattt gtcttatgaa aactagcccg tgttaggaat
     2221 gacctgggtc cagcgcccaa gggtgcccgc gttacaatgc gtcgcgccca ttccctgcgc
     2281 attcaatccc ttctgatagt ttcattccca tgctcgccac cgattccgat ccgatcgtcg
     2341 ccattgccac tgcacccggc cgaggcggaa tcggggtcgt gcgaatttcg ttcggccgag
     2401 ctggggaagc tgccgcgcag ccgttgatgc aaacgctcac cggccaggca ttggcgccgc
     2461 gtcacgcgag ttatgtgcct ttcctcgacg acgccggcaa cgcgctcgac cgcggcatcg
     2521 cgctgtactt tcccgcgccg cactcctaca ccggtgagca cgtgctcgaa ttgcaaggcc
     2581 acggcggtcc ggtcgtgctg caactggtgc tgcaacgttg catcgaggcc ggccgcgcgt
     2641 tcggtctgcg tctggccgag ccgggcgaat tcacgcggcg cgcgtttctc aacgacaagc
     2701 tggatctggc gcaagcggaa gccgtcgccg atctgttcga ggccagcacc gaagccgcgg
     2761 cacgctcggc aggccgctcg ctcgacggcg cgttttcgcg cgacatccat gcgctggtcg
     2821 aagaagtcat cacgctgcgg atgctggtcg aagcgacgct cgattttccg gaagaggaaa
     2881 tcgactttct cgaagccgcc gacgctcgcg gcaagctcgc gcgaatccgc gagcgcctgg
     2941 cccacgtgct gagtgaagca cgtcagggtg cgttgttgcg cgagggtttg tcggtggtgc
     3001 tggcgggaca gccgaacgtc ggcaagtcgt cgttgctgaa cgcgctggcc ggcgcggagt
     3061 tggccatcgt cacgccgatc gccggcacga cgcgcgacaa ggtcgcccag acgattcaga
     3121 tcgaaggcat tccgctgcat gtgatcgaca cagccggcct gcgcgatacc gaagacgagg
     3181 tggagaagat cggcattgcg cgcacgtgga acgagatcga acgcgcggac gtcgtgctgc
     3241 atctgctcga tgcgcgaacc ggcatgaacg tcgaagacga aacaatcgcg gcgcgtttcc
     3301 cgagcggtgt gccggtggtg cgcgtgctga acaagacgga cttgaccgag ctcccgccga
     3361 ccgtgacccc gctggacgcc gatctcgatc tcagcgaagt gcggctgtcg gcgaaacaag
     3421 gcgatggcgt tgcgttgctg cgcgaggagt tgctgcgcat cgccggctgg caggccggcg
     3481 cggaaagcgt gtacctcgcg cgcgaacggc atctgattgc gctgcgcgcg gctgaggagc
     3541 atctggcgac ggctgccgcg cacgccgatc agaacgctca agc
//
LOCUS       DATASH010000283         2079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3004864,
            whole genome shotgun sequence.
ACCESSION   DATASH010000283 DATASH010000000
VERSION     DATASH010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3004864"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..1140)
                     /locus_tag="VHV99_07900"
     CDS             complement(94..1140)
                     /locus_tag="VHV99_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-keto-pentose/UDP-xylose
                     synthase"
                     /protein_id="HEX3635397.1"
                     /translation="MKKVLILGVNGFIGHHLSKRILETTDWEVFGMDMQRDRLGDLAN
                     HERMHFFEGDITINKEWVEYHIKKCDVILPLVAIATPATYVKQPLRVFELDFEANLPI
                     VRSAVKYGKHLVFPSTSEVYGMCTDEQFDPEESLLSYGPINKPRWIYACSKQLMDRVI
                     WGYGMEGLNFTLFRPFNWIGPGLDSIYTPKEGSSRVVTQFLGHIVRGENISLVDGGAQ
                     KRAFTDIDDGIGALMKIIENKDGIATGKIYNIGNPTNNFSVRELAHKMLKLAAEFPEY
                     AATAKQVQLVETSSGAYYGAGYQDVQNRVPKIDNTMQELNWAPKSTFDEALRKIFEAY
                     RGHVGDARALVEQQ"
     gene            complement(1137..>2079)
                     /locus_tag="VHV99_07905"
     CDS             complement(1137..>2079)
                     /locus_tag="VHV99_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018432095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formyltransferase"
                     /protein_id="HEX3635398.1"
                     /translation="RCLQVLLARGVEVALVVTHEDNPDENIWFGSVKSVAEDHGITVV
                     TPADPKSPELHDAISELAPDFIFSFYYRHMLPVGLLALAARGAYNMHGSLLPKYRGRV
                     PTNWAVLNGETETGATLHEMAAKPDAGAILAQTPVPILPDDTASQVFDKTTVAAEQTL
                     WRVLPALIAGEAPHLPNDLTKGSYFGGRKPEDGRIDWSQTAQQVYNLIRAVAPPYPGA
                     FTDIGQQRFIVARARLVPENGVPGIAAESLREVSNLPPGLHVKDNALFGVCGDSRVVA
                     IRELRQPFVDESQTPAGGERVVSPAEFGRLTQSIPHS"
BASE COUNT          376 a          617 c          657 g          429 t
ORIGIN      
        1 tcgaagatgc gcgcgagatt cggcacgccc tcgcgggtgc cgcgcagcgt gtcgacgtcg
       61 atcttcagaa cgatacgggc caaggatcag cccttattgc tgttcgacga gtgcgcgggc
      121 gtcgccgaca tggccgcgat acgcttcgaa aatcttgcgc agcgcttcgt cgaaagtcga
      181 cttcggcgcc cagttgagtt cctgcatcgt gttgtcgatc ttcggcacgc ggttctgcac
      241 gtcctggtag cccgcgccgt agtacgcgcc cgacgacgtt tcaacgagct gcacctgctt
      301 ggccgtcgcc gcgtactccg ggaattcggc ggcgagcttc aacatcttgt gcgcgagttc
      361 acgcacggag aaattgttgg tcgggttgcc gatgttgtag atcttgcccg tagcgatgcc
      421 gtccttgttc tcgatgatct tcatcagcgc gccgatgccg tcgtcgatat ccgtgaacgc
      481 gcgcttctgc gcgccgccgt cgacgaggct gatgttctcg ccgcgcacga tgtggccgag
      541 gaactgcgtg accacgcgcg agctgccttc cttcggcgtg tagatcgagt cgaggcccgg
      601 gccgatccag ttgaacggac ggaacagcgt gaagttcagg ccttccatgc cgtaacccca
      661 gatcacgcgg tccatcaact gcttcgaaca cgcgtagatc cagcgcggct tgttgatcgg
      721 gccgtagctc aacagcgatt cttcgggatc gaattgctcg tcggtgcaca tgccgtacac
      781 ctcggacgtg gacggaaaca cgagatgctt gccatatttc acggctgaac gaacgatggg
      841 caggttcgct tcgaagtcga gttcgaacac gcgcagcggc tgcttcacgt aggtggccgg
      901 cgtggcaatg gcaaccagcg gcaggatcac atcgcacttc ttgatgtggt attcgaccca
      961 ctccttgttg atcgtgatgt cgccttcgaa gaaatgcatg cgctcatgat tggcgaggtc
     1021 gccgagacgg tcgcgctgca tgtccatgcc gaagacttcc cagtcggtcg tctcgagaat
     1081 gcgcttggag aggtggtggc caatgaagcc gttgacaccc aggatcagga cttttttcat
     1141 gaatgaggaa ttgattgagt gagtcggccg aattcggcgg gactgacgac gcgttcgccg
     1201 ccggctggag tctggctttc gtccacgaac ggttgccgca attcacggat ggcgacgacg
     1261 cggctgtcgc cgcatacgcc aaacagcgca ttatccttca cgtgcaggcc agggggcaaa
     1321 ttcgacacct cacgcaggct ctcggcagcg atgcccggca cgccgttttc ggggacgaga
     1381 cgagcccgcg cgacaataaa ccgctgctgg ccgatgtccg taaaagcccc tggatacggc
     1441 ggcgcgacgg cgcgaatcag gttgtagacc tgctgcgccg tttgcgacca gtcgatacgg
     1501 ccatcctccg gcttccggcc gccgaaatag ctgcctttcg ttagatcgtt cggcaaatgc
     1561 ggcgcctcgc ctgcgatcaa ggccggcaac acgcgccaca aggtttgttc ggcggcgacc
     1621 gtggttttgt cgaagacttg cgaggcggtg tcgtcgggga gaatggggac gggcgtctgg
     1681 gcgagaattg ctcccgcgtc gggttttgcg gccatctcat gcagcgtcgc gcccgtttct
     1741 gtttctccgt tcagcacggc ccaattggtg ggcacgcgtc ctcgatactt cggcaacaac
     1801 gaaccatgca tgttgtatgc gccacgagcg gcgagcgcga gcaggccgac tggcagcatg
     1861 tgccggtagt agaaggagaa aatgaagtcc ggcgcgagtt cgctgatcgc gtcatgcagt
     1921 tcgggcgact tggggtcggc cggcgtgacg accgtgatgc catgatcctc tgcgactgac
     1981 ttcacactgc cgaaccagat gttctcgtcc ggattgtctt cgtgcgtgac gacgagcgcg
     2041 acttccacgc cgcgcgcgag caagacctgc aggcagcgc
//
LOCUS       DATASH010000284         1504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3004891,
            whole genome shotgun sequence.
ACCESSION   DATASH010000284 DATASH010000000
VERSION     DATASH010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3004891"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..387)
                     /locus_tag="VHV99_07910"
     CDS             complement(19..387)
                     /locus_tag="VHV99_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635399.1"
                     /translation="MSIQWGRVLLTAFLMEIILIAIAVPLSLGGAGRVLVYVIPPTAF
                     IATFGVTVWLGRGINSQLVLHGVLIGIVGTLMYMALTRAQPEPWQYWLAHGLKIAGGA
                     AGGMLLASRRAAGNALPSAP"
     gene            complement(416..1459)
                     /locus_tag="VHV99_07915"
     CDS             complement(416..1459)
                     /locus_tag="VHV99_07915"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX3635400.1"
                     /translation="MRYGYWLPVFGGWLRNVADENMQATWAYVKRLAQRSEQIGFDVT
                     LIAELNLNDIKGVEAPSLDAWSTAAALAAVTERIELMVAVRPTFHLPALLAKQAANID
                     HISGGRLTLNVVSSWWADEAKKYGVGFEQHDDRYARTGEWLDVLNGVWSQHHFSFAGR
                     YYRVEDNVLEPKPLAQPRPTLYAGGESPAAKDLIAEKCDAYLMHGDPPERVREKIADL
                     RERRAKRNLPPLKFGVAGYAIVRETEREAQDELRRITDVQQSAAGYANYQQWLAGTQL
                     EQRVSLEDYSVSNRGLRSGLVGTAEQVAERVAKFEVAGVDLMLLQFSPQMEEMERFAE
                     TVIRRGSAASALA"
BASE COUNT          283 a          515 c          413 g          293 t
ORIGIN      
        1 cgggcgtgtg tactttgatc agggcgcgga gggcagcgca tttcctgcgg cccgacggct
       61 ggccaagagc attccgccag cggcaccgcc ggcaatcttc aatccgtggg cgagccagta
      121 ttgccatggc tccggctgtg cacgggtcag cgccatgtac atgagcgtgc ccacgatgcc
      181 gatgagcacg ccgtgcagca ccaactggga attaatcccg cgcccgagcc acaccgtaac
      241 cccaaatgtg gcgatgaacg ccgtcggggg aatgacgtac accagcacgc gacccgcgcc
      301 gccgagtgac agcggcacgg caatagcgat gaggatgatt tccatcagga aagctgtcag
      361 taagacgcgt ccccattgaa tcgacatggt tctgtctaac ctcgttctgc gcgctctagg
      421 ccagcgccga tgccgcgcta cctcgccgaa tcacggtctc cgcgaagcgt tccatttctt
      481 ccatctgcgg gctgaattgc agaagcatca ggtccacgcc ggccacttca aatttcgcca
      541 cgcgctcagc gacttgctcc gccgtgccca ccaggcctga ccgcaggccg cgattggaaa
      601 cggaatagtc ctctagcgaa acgcgctgct cgagttgcgt gccggccagc cactgctggt
      661 aattcgcgta gcccgccgcc gattgctgca cgtcggtgat gcgccgcagc tcgtcctgcg
      721 cttcgcgctc ggtttcccgc acgatggcat agccggcaac gccaaatttt agcggtggca
      781 ggttgcgctt ggcgcggcgt tcgcgcaggt ccgcaatttt ctcgcgcacg cgttccggag
      841 gatcgccgtg catcagatac gcatcgcatt tttcggcgat caagtctttc gcggccggcg
      901 attcgccccc cgcgtacaag gtcgggcgtg gctgcgcgag cggctttggc tccagtacat
      961 tgtcttccac ccgataatat ctcccggcga acgaaaagtg atgctgcgac cacacaccgt
     1021 taagaacgtc cagccactcg ccagtccgcg cgtagcgatc atcgtgttgc tcgaacccca
     1081 cgccatattt cttcgcttca tccgcccacc aggacgacac cacattcagg gtcagccggc
     1141 cgccgctgat atgatcgata ttggcggctt gcttggccag cagcgccggc aaatgaaagg
     1201 tcggccgcac cgccaccatc aactcaatgc gctccgtcac cgcagccagc gccgcagcgg
     1261 tcgaccacgc atccaacgac ggcgcttcca cccccttgat gtcgttcaag ttcagttcgg
     1321 cgatcagcgt gacgtcgaat ccaatctgtt cgctgcgttg tgccagccgc ttcacatacg
     1381 cccacgtcgc ctgcatattt tcatccgcaa cattgcgcag ccacccacca aaaacgggaa
     1441 gccagtatcc gtatctcatg ctttttctcc ggccgccacc agccgcgcag cttgcttacc
     1501 ttcg
//
LOCUS       DATASH010000285         6831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5972438,
            whole genome shotgun sequence.
ACCESSION   DATASH010000285 DATASH010000000
VERSION     DATASH010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6831
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5972438"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1250)
                     /locus_tag="VHV99_07920"
     CDS             complement(<1..1250)
                     /locus_tag="VHV99_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX3635401.1"
                     /translation="MSATPPTATEVEHDADSPSSARKYIDAVLEQSFRHLFGPTATPE
                     QPRRHDVVSIAKLTSALSGYLQPEEIKDIKAAFHFSDEAHLGQYRQSGEPYITHPVAV
                     AEICAGWNLDAQSIMAALLHDVMEDQGVTKAELAERFGAKVAELVDGLSKLDKMEFRN
                     REEAQAENFRKMLLAMARDVRVILVKLADRLHNMRTLGAVPHEKRRRVARETLDIYAP
                     IAHRLGLNNTYRELQDLSFANFNPHRYATLEKAVKAARGNRREVVGKILESVQRAIAD
                     AKLDAEVTGREKTIFSIYKKMRDKQLSFSQVLDVYGFRVVVESALECYTCIGALHALY
                     KPVPGKFKDYIAIPKVNGYQSLHTTLVGPFGAPIEFQVRTRKMHEIAEAGVAAHWLYK
                     NGGADLNDVQKRAHQWLKSLLDIQ"
     gene            complement(1375..1578)
                     /gene="rpoZ"
                     /locus_tag="VHV99_07925"
     CDS             complement(1375..1578)
                     /gene="rpoZ"
                     /locus_tag="VHV99_07925"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEX3635402.1"
                     /translation="MARITVEDCLKQIPNRFELALAATYRARQLAQGHTPKIESRDKP
                     TVVALREIAAGQVGVEMLKKVPV"
     gene            complement(1720..2400)
                     /gene="gmk"
                     /locus_tag="VHV99_07930"
     CDS             complement(1720..2400)
                     /gene="gmk"
                     /locus_tag="VHV99_07930"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX3635403.1"
                     /translation="MTDHTREHSAPRNPYAGAYPGNLFMVVAPSGAGKSTLVNALLAG
                     DDAIRLSISYTTRPPRPKEQDGEHYHFTTVDDFLTRHAEGEFLESAEVHGNYYATSRV
                     WIEEQMKSGHDVLLEIDWQGAQQVKKQFHNAVEIFILPPSLDALEERLKKRGQDEPNV
                     ITRRLLAAGSEMAHAAEAEYVVINENFDRALGELQCLVAATRSRFASQYARHTQLFVQ
                     LGIHLPHA"
     gene            complement(2419..3342)
                     /locus_tag="VHV99_07935"
     CDS             complement(2419..3342)
                     /locus_tag="VHV99_07935"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017333042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEX3635404.1"
                     /translation="MIYSMTGYASATRELVAASGTGGVSVSVELRTVNSRFLDLNFRM
                     PEDVRVSEPTLREMLMNKLSRGKVDIRINLQRSEQSANAGSVNRDALTQLALLERTVL
                     STFPEAGRLRTGEILRWPGVLAETGVAPEVLRDAVLACGKQAITDLIDVRAREGAQLA
                     AMLLANVTEMEAIVTKITPLVPELIARHQQKIVERLQEALGIAAPDTAATLVSREEIN
                     ERIRQEVTMYGIRIDIAEELSRLTAHLNETRHVIEKGGKVGKRLDFMMQELNREANTL
                     GSKAAAKELADSSMTLKLLIEQMREQVQNLE"
     gene            3609..4349
                     /gene="rph"
                     /locus_tag="VHV99_07940"
     CDS             3609..4349
                     /gene="rph"
                     /locus_tag="VHV99_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEX3635405.1"
                     /translation="MTNHTQRPSGRQANQLRDVRITRHYTKHAEGSVLVEFGDTKVIC
                     TASIAESVPSFLRDRGQGWLTAEYGMLPRATHTRSDREAARGKQTGRTQEIQRLIGRA
                     LRSVFDLEKLGARTLHIDCDVIQADGGTRTASITGAFVAAHDAVARLLATGRIESSPI
                     SDYVAAISVGVYDGLPVLDLDYDEDSQCDTDMNVVMTGAGGFVEIQGTAEGVPFSRDE
                     MNALLDLASDGINTLIAKQKEALEQKSE"
     gene            4342..4989
                     /gene="rdgB"
                     /locus_tag="VHV99_07945"
     CDS             4342..4989
                     /gene="rdgB"
                     /locus_tag="VHV99_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEX3635406.1"
                     /translation="MSEVRQGNGSVASDSPLKKVVLASNNAGKLREFAALLGAAGIDL
                     IPQGELNVPEAEEPYPTFVENALTKARHAARLTGLPALADDSGLCVRALRGAPGVYSA
                     RYAQLAGGEKSDAANNAQLVAALQGETDRRAYYYCVLALVRHADDPEPLIAEGRWHGE
                     MLDAPRGAHGFGYDPYFFLPSLNASAAELEPAVKNASSHRAIALRQLLARLTEEA"
     gene            4986..6215
                     /gene="hemW"
                     /locus_tag="VHV99_07950"
     CDS             4986..6215
                     /gene="hemW"
                     /locus_tag="VHV99_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEX3635407.1"
                     /translation="MIPIKPAPADIGNGVIKAFTSPGSIRLTSLPPLALYVHFPWCVR
                     KCPYCDFNSHEWKGDTFPETEYLDALRADLEMALPLVWGRQVHTVFIGGGTPSLLSAA
                     GLDRMLSDVRALLPLDADAEITLEANPGTFEAAKFAQFRASGVNRLSVGIQSFNEAHL
                     KALGRIHDSTQARHAVEVAATTFDNFNLDLMFALPQQTLAQCQADVETALSFAPPHLS
                     LYHLTLEPNTLFAKFPPALPDDDASADMQDWIHERTTAAGYGRYEVSAYAQPHRQSKH
                     NLKYWRFGDYLGIGAGAHTKLSFPNRVLRQARYKHPATFIEQAKAGTAVQEEHEVGPR
                     DLPFEFMLNALRLVEGFPVHRFIERTGMSMTSIEPALQEAERRKLITRDHEKIAPTPL
                     GQAFLNDLQELFLKDPQ"
     gene            6271..6360
                     /locus_tag="VHV99_07955"
     tRNA            6271..6360
                     /locus_tag="VHV99_07955"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6305..6307,aa:Ser,seq:tga)
     gene            complement(6455..>6831)
                     /locus_tag="VHV99_07960"
     CDS             complement(6455..>6831)
                     /locus_tag="VHV99_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635408.1"
                     /translation="EAQMKILAGVCSVIALAWLFVTTSVPRAPVVQPCTQEWFSYLDS
                     HYFDISDGEGHGPDLGNSEWFNAFEERAKLPVTNRLPKQQRCRLVQGQLERHTYIINE
                     QLGWTISLERATGSKRQEKSPG"
BASE COUNT         1256 a         2109 c         2094 g         1372 t
ORIGIN      
        1 ctctgaatgt cgagcagcga cttgagccat tgatgcgcgc gcttctgcac atcgttcagg
       61 tccgcgccgc cgttcttgta caaccagtgc gcggcgacac ccgcttcggc gatctcgtgc
      121 atcttgcgcg tgcgcacctg gaactcgatc ggcgcgccga acgggccgac caaggtggta
      181 tgcagcgact gatagccgtt caccttcggg atcgcgatgt aatccttgaa cttgcccggc
      241 accggcttgt agagcgcatg cagcgcgccg atgcaggtgt agcattccaa cgcactttcg
      301 accaccacgc ggaagccgta cacgtcgagc acctgcgaga acgacaactg cttgtcgcgc
      361 atcttcttgt agatgctgaa gatggtcttt tcgcggccgg tgacttcggc gtcgagcttc
      421 gcgtcggcaa tcgcgcgctg caccgattcg agaatcttgc cgaccacttc acgccggttg
      481 ccgcgcgcgg ccttgacggc tttctcgagc gtggcgtacc gatgcggatt gaagttcgcg
      541 aagctcaggt cctgcagctc gcgataggta ttgttcaggc caagccgatg ggcgatcggc
      601 gcgtagatgt ccagcgtttc gcgcgccacg cggcgacgct tttcatgcgg caccgcaccc
      661 agcgtgcgca tgttgtgcaa gcggtcggcg agcttcacca gaatcacgcg gacgtcgcgc
      721 gccatcgcga gcagcatttt gcggaagttc tccgcctgtg cctcttcgcg attgcgaaac
      781 tccatcttgt ccagtttcga caagccgtcg accagttccg cgaccttcgc gccgaatcgc
      841 tcggcgagtt cggccttggt cacgccctgg tcttccatca cgtcatgcag cagggccgcc
      901 atgatcgact gggcgtcgag attccagccg gcgcagattt ccgcgacggc gacaggatgc
      961 gtgatgtacg gttcgccgct ctggcgatac tggccgaggt gggcttcgtc gctgaaatgg
     1021 aacgccgcct tgatgtcctt gatctcttcc ggctgcaaat agccggacaa ggcggacgtc
     1081 agcttggcga tcgaaacgac gtcatgccgg cgcggttgct ccggcgtggc ggtcggcccg
     1141 aacagatggc gaaacgactg ttcgaggacc gcgtcgatgt acttccgtgc agacgagggc
     1201 gaatcggcgt cgtgttccac ttccgtggcg gtgggcgggg tagcactcat tttcgcctcc
     1261 gtagcggtta gggttggacg ccggtctgca ccttgatgca attacatgta gcagccaggt
     1321 gtggcaaatg cgggtaaatc aaaacgcaaa aacacgaaat cggcacagtg cgccttaaac
     1381 cggcaccttc ttcagcattt cgacgccaac ctggccagcc gcgatttcac gcaatgcgac
     1441 gaccgtgggc ttgtcgcggc tttcgatctt cggcgtgtga ccttgcgcga gctgacgtgc
     1501 gcgataggtc gcggcaagcg cgagttcgaa acggttcggg atctgtttga ggcagtcttc
     1561 gacggtaatg cgggccatgt tgggttcctt cttgcaagaa aacgctgggc cgccccaagt
     1621 tttcttgtcc ccctcggggg gcctggcgcg aagcgacagg tctgggggcg ttaattgatg
     1681 ttgcttattc taccttatgt gctcggcaca ccgccgcgct cacgcgtgtg gcaggtggat
     1741 gccgagctgc acgaaaagct gcgtgtggcg cgcgtattgc gaggcgaagc gcgaacgcgt
     1801 cgccgccacg aggcactgca gttcgccgag cgcgcgatcg aagttctcgt tgatcacgac
     1861 atattccgct tcggccgcat gcgccatctc gctaccagct gcgagaagac gccgcgtgat
     1921 cacgttcggt tcgtcctggc cgcgcttttt cagacgctct tcgagcgcat cgagcgacgg
     1981 cggcaagatg aaaatttcaa cagcgttgtg aaactgtttc ttcacctgtt gcgcgccttg
     2041 ccaatcgatt tcgagcagca cgtcatgccc gcttttcatc tgctcttcga tccacacgcg
     2101 cgaagtggcg tagtagttgc cgtgcacttc ggcgctttcc agaaactcgc cttccgcgtg
     2161 acgcgtcagg aaatcgtcga cggtcgtgaa gtgatagtgc tcgccgtcct gctccttcgg
     2221 acgcggcggg cgcgtcgtgt acgaaatcga cagacggatc gcgtcgtcgc cggcgagcag
     2281 cgcgttcacg agcgtcgact tgcccgcgcc cgatggcgcg acaaccatga acagattgcc
     2341 ggggtaagcc ccggcgtaag gattgcgcgg tgcgctatgt tcgcgtgtat ggtcggtcat
     2401 gttcgtgttg ctccagcctt attccagatt ttgtacttgc tcgcgcatct gctcgatgag
     2461 caacttcagg gtcatcgacg aatccgccag ttccttcgca gccgccttcg aaccgagcgt
     2521 attcgcttcg cgattcagtt cctgcatcat gaagtccagg cgcttgccga ccttgccgcc
     2581 cttctcgatc acgtggcgcg tttcgttcag gtgagccgtg agacgcgaca gttcttcggc
     2641 gatatcgatg cggatgccgt acatcgtcac ttcctggcga atccgttcgt tgatttcctc
     2701 gcgcgagaca agcgtcgccg cggtatcggg cgcggcgatg ccgagcgctt cctgcagacg
     2761 ctcgacgatc ttctgctgat gcctcgcgat cagttccggc acgagcggtg tgatcttcgt
     2821 gacgatcgct tccatttcgg tgacgttggc gagcagcatg gcggcgagtt gcgcgccttc
     2881 gcgggcacgc acgtcgatca gatcggtaat cgcctgcttg ccacatgcga gcaccgcatc
     2941 gcgcagcact tccggtgcca cgccggtttc ggccagcacg cccggccagc gcagaatttc
     3001 gccggtacgc aggcgccctg cttccggaaa cgtcgacaac accgtgcgct cgagcaacgc
     3061 cagttgcgtc agcgcgtcgc gattgaccga gcccgcgttg gccgactgct cgctgcgctg
     3121 caaattgata cgaatgtcga ccttgccgcg cgagagcttg ttcatcagca tttcgcgcaa
     3181 cgtcggttca gagacgcgca cgtcttccgg catccggaag ttcagatcga gaaagcgcga
     3241 attcacggtg cgcagttcaa ccgatacgct cacgccacct gtgcccgagg ccgcgacgag
     3301 ttcgcgcgtg gcgctcgcat agccagtcat gctgtagatc atcgtgtttc tcgcgattgt
     3361 ggccatgcgt tcgacgatgg ccggagagtc gaagcgcccg gcgtttacga gacgcggggc
     3421 ggagaatcca cattatccca tttttgccgg cgagcccgct tccagcgagg gcagcacgca
     3481 taaagcgctg cctcgcgcag agccccgcta catgcgggca ggcgtcccgg cgaccggctg
     3541 ccccgcgccc gccgcgatca gcggtaaaat tgcgctttcc ctcctgcctg tcttccagac
     3601 catccaacat gaccaaccac acccaacgcc ccagcggccg ccaggccaat cagctgcgcg
     3661 acgtgcgcat cacgcgccac tacacgaagc atgcggaggg ctcggtgctg gtcgaattcg
     3721 gcgacaccaa ggtcatctgc acggcgagca tcgccgaaag cgtgccgtcg ttcctgcgcg
     3781 accgtggcca gggctggcta accgccgaat acggcatgct gccgcgcgcc acgcacaccc
     3841 gcagcgatcg tgaagccgcg cgtggcaagc aaaccggccg cacccaggaa atccagcgtc
     3901 tgatcggccg cgcactgcgc tcggtgttcg atctcgagaa actcggcgcg cgcacgctgc
     3961 atatcgattg cgacgtgatc caggccgacg gcggcacgcg cacggccagc attaccggcg
     4021 cattcgtggc cgcccatgac gcggtggcca ggctgctggc tacggggcgc atcgaaagct
     4081 cgccgatcag cgactatgtc gcggcgattt cagtcggcgt gtacgacggt ctgccggtgc
     4141 tggatctcga ctacgacgaa gattcgcaat gcgacaccga catgaatgtg gtgatgacgg
     4201 gcgcgggcgg cttcgtcgaa attcagggca cggccgaagg cgtgcctttc tcgcgcgatg
     4261 aaatgaatgc gctgctcgat ctggcgagcg acggcatcaa cacgctgatc gcgaagcaga
     4321 aagaagcgct ggagcaaaag agtgagtgaa gttcgtcaag gcaacggctc ggttgcgtct
     4381 gactcgccgt tgaagaaagt cgtgctggcg tcgaacaacg cgggcaagct gcgcgagttc
     4441 gcggcgctgc tcggcgcggc cggcatcgac ctgattccgc aaggcgaact gaacgtgccg
     4501 gaagcggaag agccgtatcc gactttcgtc gaaaacgcgc tgaccaaggc acgtcatgcg
     4561 gcgagactca ccggcctgcc cgcgctcgcc gacgattccg gcttatgcgt gcgcgcgctg
     4621 cgtggcgcgc cgggcgttta ttcggcgcgt tacgcgcaac tcgcgggcgg tgaaaagagc
     4681 gacgcggcta acaacgcgca gctcgttgca gctttgcaag gtgagaccga tcgacgcgcg
     4741 tattactact gcgtgctggc actcgtgcgt catgcggacg atcccgaacc gctgatcgcc
     4801 gaaggccgct ggcacggcga aatgctggat gcgccgcgcg gcgcacatgg tttcggctat
     4861 gacccgtact tcttcctgcc gtcgctgaat gcgagcgccg cggaactcga accggcggtg
     4921 aagaacgcca gcagccatcg cgcgattgcg ttgcgccaac tgctcgcgcg tttgacggag
     4981 gaagcgtgat tccgatcaaa ccggcgcccg ccgatatcgg caacggtgtc atcaaggcat
     5041 tcacgtcgcc aggcagcatt cgcctgacgt cgttaccgcc actggcgctg tatgtgcact
     5101 ttccgtggtg cgtgcgcaaa tgcccgtact gcgacttcaa ctcgcatgag tggaaaggcg
     5161 acaccttccc ggaaaccgag tatctcgacg cgctgcgcgc cgatctcgag atggcgctgc
     5221 cgcttgtgtg ggggcgtcag gtgcatacgg tgttcatcgg cggcggtacg cccagcttgc
     5281 tgtcggcggc cgggctcgac cggatgctgt ccgacgtgcg cgcgctgctg ccgctcgacg
     5341 ccgatgccga aatcacgctc gaagccaacc cgggtacctt cgaagccgcc aagttcgcgc
     5401 agtttcgcgc gagcggcgtg aatcgcctgt cggtgggcat ccaaagtttc aatgaagcgc
     5461 atctgaaggc gctcggccgg attcacgatt cgacgcaagc acgccatgca gtcgaagtgg
     5521 ccgcaaccac cttcgataac ttcaatctcg atctgatgtt cgcgctgccg cagcagacgc
     5581 tcgcacaatg ccaggcggat gtggagacgg cgttgtcgtt cgcgccgccg catttgtcgc
     5641 tgtatcactt gacgctcgaa ccgaacacgc tgtttgcgaa attccccccc gcgctccctg
     5701 acgacgatgc atccgccgac atgcaggact ggattcacga acgcacaacg gctgccggct
     5761 acggacgcta cgaagtctcc gcgtatgcgc agccgcatcg gcagagcaaa cacaatctga
     5821 agtactggcg ttttggcgac tatctcggca tcggcgcagg tgcgcacacc aagctgtcgt
     5881 ttccgaaccg cgtgctgcgt caggcgcgct acaagcatcc ggccaccttc atcgagcagg
     5941 ccaaggccgg cacggcggtg caggaagagc acgaagtcgg gccgcgcgat ctgccgttcg
     6001 agttcatgct gaacgcgttg cggctggtgg aagggtttcc ggtgcatcgg tttatcgagc
     6061 gcacggggat gtcgatgacc tcgatcgagc cggctttgca ggaggcagaa cggcgcaagc
     6121 tgattacacg cgaccacgag aagatcgcgc cgacgccgct tggccaggcc ttcctgaacg
     6181 atctgcaaga actgtttctg aaagatccgc agtgattgcg acctcacgga gcaccgcaac
     6241 gaggattttt caggttacaa tttagggctt ggaggtgtgg ccgagtggtt taaggcaccg
     6301 gtcttgaaaa ccggcgaagg agtgatcctt ccgtgagttc gaatctcacc gcctccgcca
     6361 gaattccaca caaaaccccg caagttcatt gacttacggg gtttttgttt tttctggtcc
     6421 atcaaacagc ccatcatcgg gcaaagtttc acggctatcc cggcgatttc tcttgccgct
     6481 tgctccccgt cgcgcgttca agtgagattg tccagccaag ctgttcgttg atgatgtaag
     6541 tatggcgctc aagctggccc tgaaccagtc ggcagcgttg ctgttttgga agacgatttg
     6601 tcaccggcag cttggctctt tcctcaaatg cgttaaacca ttcactgttg cccaagtcag
     6661 gtccgtgccc ttccccatct gagatatcga agtagtggct gtcgaggtat gaaaaccact
     6721 cttgcgtaca cggctggacg actggggcac ggggtacgct ggtagtaacg aacagccacg
     6781 cgagagcgat aacggaacag acgccagcga ggattttcat ttgcgcctct t
//
LOCUS       DATASH010000286         4156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5775188,
            whole genome shotgun sequence.
ACCESSION   DATASH010000286 DATASH010000000
VERSION     DATASH010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5775188"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(460..1527)
                     /locus_tag="VHV99_07965"
     CDS             complement(460..1527)
                     /locus_tag="VHV99_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEX3635409.1"
                     /translation="MKLRTTVLALTTTGLISGCATGPDRKPGDPFEPANRVIFNFNDG
                     IDRYVAVPVAKGYQKVTPQPLRTAVSNFFSNLGDLTNAANNLLQLKITDATEDIVRFA
                     FNSTFGLGGLLDWATPAGLPKHHQDFGLTLGHWGVPSGPYLVLPLFGPSTVRDSMGLI
                     VDVKFNPLNYIEPATRNPLYVLQFVSTRSDLLGASNLLEQAALDKYSFVRDAYSQQRR
                     ARLRGTGENAAPLPNYDDQGGDSGAAAPAAGAPAALPNYADPGDAAQAPNAASGSATG
                     APAGVPNFTDPGDTPPAPNAASGSAPGAAPGVPNYTDPGETPNTPAASPAAASGATTP
                     APAPQTVPTDKKPQQAIPATQ"
     gene            complement(1530..4145)
                     /locus_tag="VHV99_07970"
     CDS             complement(1530..4145)
                     /locus_tag="VHV99_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX3635410.1"
                     /translation="MLKSTIVRLVAYSVRHPLRIIALSLVLAVLSGFYVAHNFKINTD
                     ISKLIETDKQWSSLENAMDEAFPDRGQTVLVVVEARAPEFADAAANALTAALKADPKE
                     FVAVSQPAGGPFFEHNGLLFPATGEVLSTTSQLVQSRPLVNALAHDPSLTGLAGTLTT
                     SLLLPLQLGQVKLADMSHLLSQSAITLDHVLAGQPAAFSWRALVDKSAATEPARAFVT
                     VQPVVNYDALEPGATASKSIRDTAASLHLDTRYGATVRLTGEQPLADEEFASVKDGAV
                     LNGIGTFIVVLIILWLALRSGRMIVAVFITLFVGLAITAALGLLLVGALNMISVAFMV
                     LFVGLGVDFGVQFGVKYREERHRDDRLSAALVHTAHSIGVPLTLAAVAVALSFFSFLP
                     TAYRGVSELGEIAGVGMFVAYFTNMTLLPALLKIFNPPGEVASPGFQQLAPVDDFLDR
                     HRKPVLIATLIIVIGATPLLTHLRFDFNPLHLKDPHTESMETLLSLKDSPEAAVNNVH
                     VLAPTLADADQKAAHLRTLPEVGRVTTLDSFIPAEQQQKLMLIASAAQQLLPALQQQP
                     APQATDAVRVAALKRASNQLSLAADDHPGPGAAEARHLSATLQKLAAADAATRDRAET
                     AMSEPLRIALKQLADLLQPSEITRDNLPREISTSWVSKDGRALVDIAPKVKAGTDPND
                     DATLARFAHAVKKAEPDAIGGPISILHSADTIIKAFLQAAGYALLSIAILLWIALRRV
                     GDMLRTLVPLLVSALVTLELCVVFGMPLNFANIIALPLMLGVGVAFKIYFVMAWRNGQ
                     TGLLQSSLTHAVLFSAATTATAFGSLWLSHHPGTSSMGRLLALSLFCTLIGAVVFQPV
                     LMGKPRQRRAKHKGI"
BASE COUNT          714 a         1279 c         1416 g          747 t
ORIGIN      
        1 tgctggtcgt tttttgtgga tttttatggt gttgggcttt ccttgaattg ttattggtct
       61 attagcgtcg cccctgtgcg gggcgggcac ctacttttct ttgcctgccg caaagaaaag
      121 taggcaaaag aaagcggctc acaccgtcag cgcataagcg ggtcccccgc gcagtcgcgg
      181 tagtggtgca tctggaatct gtgccctcgc gcattcggcg tgagtgacac agccatcatt
      241 cttccagcga cgcttcgcgc gccgaagcgc gtgcttccta caaccactcg tttgctccgc
      301 gtccgtattc gcaatctgcc ggcgcgtgat tcatgttcat ccgtgtagtc ggccgctcgc
      361 ggccctggct tctgtcgcgc atccgcactt cttttcggca gtgcgctctt cgggccgttc
      421 ggcacgcttg acgcgcgccg aatggacgac ccacgcccgc tattgggtcg ccggtatcgc
      481 ttgctgcggc ttcttgtccg tcggcacggt ttgcggtgcc ggcgccggtg tcgttgcgcc
      541 cgacgcggcg gcaggcgacg ccgccggggt gtttggcgtc tcgcctgggt ccgtgtagtt
      601 cggtacgccc ggcgcggcgc ccggggcgct tccggaggcg gcgttcgggg ccggcggggt
      661 gtcgcccggg tccgtgaagt tcggcacgcc tgccggagcg cccgtggcgc tcccagatgc
      721 ggcgttcggc gcttgcgctg cgtcgcccgg gtccgcgtag ttcggtaagg ccgcgggagc
      781 acccgcggcg ggagcggctg cgccggagtc gccgccctgg tcgtcgtagt tcggcagcgg
      841 cgcggcgttc tcgccggtgc cgcgcaaacg cgcgcggcgc tgctggctat aggcgtcacg
      901 tacgaatgaa tacttgtcga gcgcagcctg ttccagcagg ttggatgcgc ccagcaagtc
      961 ggagcgcgtg ctcacgaact gcaacacgta cagcggattg cgtgtggccg gctcgatgta
     1021 gttcaacggg ttgaacttca cgtccacgat cagacccatg ctgtcacgca ccgtgctcgg
     1081 gccgaatagc ggcaagacga ggtaggggcc cgacggcacg ccccagtggc ccagcgtcaa
     1141 gccgaagtcc tggtggtgct tcggcagacc ggccggcgtc gcccagtcga gcaggccgcc
     1201 aagtccaaag gtcgagttga acgcgaagcg cacaatgtct tcggtggcgt ccgtgatctt
     1261 cagctgcaac aggttgttgg cggcgttggt caggtcgccc agattcgaga agaagttgct
     1321 caccgcggtg cgcaagggct gcggcgtcac cttctggtaa cccttggcga ccggaacggc
     1381 gacgtagcgg tcgatgccgt cgttgaaatt gaaaatcacg cggttcgccg gttcgaacgg
     1441 gtcacccggc ttgcggtcgg ggccggtcgc gcagccggag atcagaccgg tggtggtcag
     1501 cgccagcacg gtggttcgca acttcatacc tatattcctt tgtgcttcgc gcgacgttgg
     1561 cgcggcttgc ccatcagcac gggttgaaac accaccgcgc cgatcagcgt gcaaaacaac
     1621 gagagtgcca gcaagcgtcc catgctcgac gtgcccggat gatgcgacag ccagaggctg
     1681 ccgaacgccg tcgcggtggt cgccgcgctg aacaacacgg catgcgtgag gctcgactgc
     1741 aaaaggccag tctgcccgtt gcgccacgcc atcacgaagt aaatcttgaa tgctacgccg
     1801 acgccgagca tcagcggcaa cgcaatgatg ttcgcgaagt tcagcggcat gccgaacacc
     1861 acgcacaact ccaacgtcac cagcgccgat accagcagcg gaaccagcgt gcgcagcata
     1921 tcgccgacgc gtcgcagtgc gatccacaac agaatcgcaa tcgacagcaa tgcatagccg
     1981 gcggcttgca ggaacgcctt gatgatcgtg tccgcggaat gcaggatcga gatcgggccg
     2041 ccgatcgcgt ccggttcggc tttcttcacg gcgtgcgcaa agcgggcgag cgtcgcgtcg
     2101 tcgttcgggt cggtgccggc cttgaccttc ggcgcgatgt cgacgagtgc gcggccgtcc
     2161 ttcgagaccc agctcgtgga gatctccctg ggcaggttgt cgcgcgtgat ctcgctgggc
     2221 tgcagcagat ccgccagttg cttcagcgcg atgcgcagcg gctcggacat cgcggtttcc
     2281 gcccggtcgc gcgtggcggc gtcggcagcg gccagctttt gcagcgtcgc ggacagatgc
     2341 ctggcctcgg cggcgcccgg gcccgggtgg tcgtcggcgg cgagtgagag ctggttcgac
     2401 gcgcgcttca acgcggcgac gcgcaccgcg tccgtcgctt gaggcgcggg ttgttgctgc
     2461 agcgcgggca acagttgctg cgcggcgctc gcaatcagca ttagcttttg ctgctgttcg
     2521 gccggaatga aactgtccag cgtggtcacg cggcccactt ccggcagcgt gcgcagatgg
     2581 gcggccttct ggtccgcgtc ggcaagcgtg ggcgccagca catgcacgtt gttgaccgcc
     2641 gcttccggtg aatccttcag cgacagcagc gtctccatgg attccgtgtg cggatccttc
     2701 agatgcagcg ggttgaagtc gaagcgcaga tgcgtgagca aaggcgtggc gccgatcacg
     2761 atgatcagcg tggcgatcag caccggcttg cggtggcgat cgaggaaatc gtcgacaggg
     2821 gcgagttgct ggaagccggg cgaagcgacc tcgcccggcg gattgaagat tttcagcagc
     2881 gccggcagca gcgtcatgtt ggtgaagtac gcgacgaaca tgccgacgcc ggcgatctcg
     2941 cccaattccg acacgccgcg gtaagccgtc ggcaggaacg agaagaagct cagcgcgacc
     3001 gccacggcag cgagcgtcag cggcacgccg atgctgtgcg cggtatgcac gagcgcggcg
     3061 gaaagacggt cgtcgcgatg acgctcctca cgatatttga cgccgaactg cacgccgaaa
     3121 tcgaccccga gcccgacgaa cagcaccatg aaggcgaccg aaatcatatt gagcgcgccg
     3181 accagcagca gaccgagcgc cgcggtgatt gcgagcccga cgaacagcgt gatgaacacc
     3241 gcgacgatca tgcggcccga acgcagtgcg agccacagaa tgatcagcac gacgatgaag
     3301 gtgccgatgc cgttgagcac ggcgccgtcc ttgaccgatg cgaattcttc gtcggcgaga
     3361 ggttgttcgc cggttagtcg gaccgtggcg ccataacgcg tatcgaggtg cagcgaggcg
     3421 gcggtgtcgc ggatcgattt cgacgcggtc gcgcccggct ccagcgcgtc gtagttcacc
     3481 accggctgca ccgtgacgaa ggcgcgcgcc ggctccgtgg cggcgctttt gtccactagc
     3541 gcgcgccacg aaaatgccgc cggctggccg gccagcacgt ggtctagcgt aattgcgctt
     3601 tgcgacagca aatggctcat gtcggccagc ttcacctgac ccagttgcag cggcagcaac
     3661 aggctcgtgg tgagcgtgcc ggcgaggccg gtcaggctcg gatcgtgcgc cagggcattg
     3721 accagcgggc gggactggac gagctgcgag gtggtcgaga ggacttcacc ggttgctggg
     3781 aacaacagac cattgtgttc gaagaacggg ccgccggcgg gctgcgagac cgcgacgaac
     3841 tccttcggat cggctttgag cgcggccgtc agggcattgg cggcggcatc ggcaaactcc
     3901 ggggcgcggg cctcgaccac gaccagcacg gtttgaccgc gatccgggaa ggcctcgtcc
     3961 atggcatttt caagcgacga ccattgcttg tcggtctcga tgagcttgct gatatcggtg
     4021 ttgatcttga agttatgggc gacatagaag ccgctcaaaa cggcgagcac gagcgacaga
     4081 gcgatgatcc gtaacggatg gcgcaccgaa taggcgacga gacggacgat agttgacttc
     4141 agcatgtagg cggaga
//
LOCUS       DATASH010000287         4344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4588881,
            whole genome shotgun sequence.
ACCESSION   DATASH010000287 DATASH010000000
VERSION     DATASH010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4588881"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="VHV99_07975"
     CDS             complement(<1..852)
                     /locus_tag="VHV99_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HEX3635411.1"
                     /translation="MPGPDSVLRQVCSDPAETWLERCIAVATAALLKQQRPDGHWIFE
                     LEADATIAAEYVVLQHFLGERNPVLEAKIGTYLRRLQGSHGGWSLLQDGAFNISATVK
                     AYFALKLIGDAPESKHMRQAREAVLAHGGAGTINVFTRILLALFGLVPWAAVPVIPVE
                     IVFLPRWFPFHLSRVSYWSRTVLVPLLVLQALKPRARNPRGVRIDELFPTGRPRARRW
                     AMAPHQSRVRFAAFAAIDAALRLVEPLIPQSIRRRAIDKAVAFVIERLNGEHGLGAIF
                     PAMANTVM"
     gene            complement(889..1746)
                     /gene="hpnD"
                     /locus_tag="VHV99_07980"
     CDS             complement(889..1746)
                     /gene="hpnD"
                     /locus_tag="VHV99_07980"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012356774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HEX3635412.1"
                     /translation="METDATSSAREPAVAGSSFYAAMRVLPAEQRQAMFAIYDFCRAV
                     DDVADGRGDRQVRLAELAGWRMDIGALFADAAPSRVRALAGPILAFDLQREDFLSIID
                     GMEMDVQSDIRAPELSTLDLYCDRVASAVGRLSVRVFGIRGQDGAALAFHLGRALQLT
                     NILRDLDEDAGKGRLYLPREALVAAGIDQTDPATVLDHPALSKACATVLERVRDNFAA
                     AGLLMAKHPLSIVRAPRLMAEVYRNILERLAARGWAPPRRPVRVARPTLLWIVLRHGL
                     LNVSPRGEM"
     gene            complement(1757..2944)
                     /locus_tag="VHV99_07985"
     CDS             complement(1757..2944)
                     /locus_tag="VHV99_07985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006029686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3635413.1"
                     /translation="MTALLIASLCCLVWAYLLAARGGFWRATQSEPALQPAVSQDVHW
                     PRVVAVIPARDEASIIDNTIVSLLRQDYRGSLTLIVVDDHSSDGTAAVARHAAATASG
                     SDRLTVLAAPSLPDGWTGKLWAMHQGVRHAQALPEPPDYLLFADADMQYAGDALTELV
                     RRAVGDRLVLVSVMARLHCQSLAERALIPAFIFFFQMLYPFAWVNRADRATAAAAGGC
                     MLAHRPTLQAVGGIEAIRGNIIDDCALARLLKGHGPIWLGMTERVRSVRANTSLADIR
                     RMVVRCAYAQLGFSPWWLAAAIVAMIVTYLAPPALALLGSGAAQALGALAWALMALAF
                     QPTLRLYAVSPWWGLVLPVIAATYVVFTLDSACQHWRGRGGEWKGRIHRRTSPREASE
                     HLL"
     gene            complement(2941..3942)
                     /gene="hpnA"
                     /locus_tag="VHV99_07990"
     CDS             complement(2941..3942)
                     /gene="hpnA"
                     /locus_tag="VHV99_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019255812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid-associated sugar epimerase"
                     /protein_id="HEX3635414.1"
                     /translation="MNEPGRVLVTGATGFVGSAIVRAFLGAGYPVRALVRTSSPRGNL
                     AGLEIEIVEGDIRDANAITRAMSGISSVVHAAADYRLWARDSKAIMRTNVEGTRTVMG
                     AALAAGVDRIVYTSTVATLTLRSGGAPADETAPLPDTVEVGAYKRSKVVAERLVEDMI
                     ARDALPAIIVNPSTPIGPRDVRPTPTGRLIVAAASGRIPGFVNTGLNLVHVDDVAAGH
                     VAAFTHGKIGERYILGGQNVLLADMLGDIARLAGQRPPRFKLPRAPLYPLAAVAEMIA
                     AITGREPLLTLEGLRMSKQRMFFSSAKAERELGFRARPYTDGLDDAIDWFREAGYLR"
BASE COUNT          876 a         1397 c         1337 g          734 t
ORIGIN      
        1 catcaccgta tttgccatcg cgggaaagat cgcgccgagg ccgtgctccc cattcagccg
       61 ttcgatcaca aacgcgactg ctttgtcgat tgcgcgccgg cgaatcgatt gcgggatcaa
      121 gggttcgacc agccgcaaag ccgcgtcgat agcggcgaag gccgcgaaac gcacgcgcga
      181 ttgatgcggc gccattgccc accgtcgcgc ccgaggacgc ccggtcggga acagctcatc
      241 gatgcgcacg ccgcgcggat tgcgcgcccg cggcttcagc gcttgcagaa ccaggagcgg
      301 caccagaacg gtccggctcc aataggagac tcgcgacagg tggaacggaa accaccgcgg
      361 caggaaaacg atttcgactg ggatgactgg caccgcagcc cagggaacca agccaaagag
      421 cgccagcagg atgcgcgtaa agacgttgat agtgccggca ccgccgtggg cgaggacggc
      481 ctcacgcgcc tggcgcatgt gcttcgattc gggcgcatcg ccgatcagct tgagggcaaa
      541 atacgctttg acggtcgcac tgatgttaaa cgccccgtcc tgcaatagtg accacccgcc
      601 gtgcgagccc tgcaatcgcc gcagataggt cccgatcttg gcttcgagga ccggattgcg
      661 ctcacccagg aagtgctgca agacgacgta ttcagctgca atggtggcgt cggcttccag
      721 ctcgaaaatc caatgaccgt ccgggcgctg ctgcttcaac agggcggccg tagccacagc
      781 gatgcagcgc tcgagccacg tctcggcggg atcgctgcag acttggcgca agacgctgtc
      841 gggacctggc atcagcaacg cctcacgggg tgaagactgc atgaacgatc acatttcccc
      901 gcgcgggctg acattgagca gtccatgtcg cagcacgatc cacagcagcg tggggcgcgc
      961 cacgcggacc gggcgccgcg gcggcgccca tccgcgcgcg gccagacgct cgagtatatt
     1021 ccgataaacc tccgccatca ggcgcggcgc gcgcacgatg cttaaggggt gcttcgccat
     1081 gagcaggccg gcggcagcga agttgtcccg cacacgctcc aaaacggtcg cgcaggcctt
     1141 gctcaacgcg gggtgatcga gaaccgtcgc cgggtcggtc tggtcgattc ccgcggcgac
     1201 aagcgcttcg cgcggcagat acagccgccc cttcccggca tcctcgtcca ggtcgcgcag
     1261 aatgttggtg agctgcagtg ctcgtccgag atggaaggca agcgctgcgc catcctgtcc
     1321 gcgtatgccg aatacgcgga cagagaggcg acccactgca gacgcaaccc ggtcacaata
     1381 taaatcaagc gtggacagct cgggagcgcg gatgtcggac tggacgtcca tttccatgcc
     1441 gtcgatgatc gagagaaagt cctcgcgttg caggtcaaac gcaagaatcg gcccggcaag
     1501 tgcgcggacg cggctcggcg ccgcatctgc aaacagtgcg ccgatatcca tgcgccatcc
     1561 cgccagctcg gcgaggcgca cttggcgatc cccccggccg tcggccacgt cgtcgaccgc
     1621 gcggcagaag tcgtagattg cgaacatggc ttggcgctgc tcagctggca gcacgcgcat
     1681 cgcagcataa aaggagctgc cggcaaccgc gggttccctg gcggatgagg ttgcgtcagt
     1741 ttccatctgc cgcggctcag agaagatgct cggaagcttc gcgcggcgag gttctgcgat
     1801 ggatgcgccc tttccattcg ccacccctgc cgcgccaatg ctggcaggcc gagtcgagcg
     1861 taaacacgac gtacgtcgcc gcgatgaccg gcagaaccaa accccaccac ggcgaaacag
     1921 cgtagagacg cagcgtcggc tgaaaagcga gcgccattag cgcccacgcc agcgcaccga
     1981 gcgcttgtgc cgcaccgctg cccagcagag ccaatgccgg cggagccagg tacgtaacga
     2041 tcatcgccac gatggcggcc gcaagccacc agggggagaa ccccagttga gcgtacgcac
     2101 agcgcaccac catccgccgg atgtcggcaa gcgacgtatt ggcgcggacg ctacgcacgc
     2161 gttcggtcat tccaagccag atcgggccat gccccttgag caatcgggcc agcgcgcagt
     2221 cgtcgatgat gttgccacga atcgcctcga tgccgcccac ggcctgcagc gttgggcgat
     2281 gcgccagcat gcacccgccg gcggccgctg ccgtcgcccg atccgcgcgg tttacccagg
     2341 cgaatgggta caacatctgg aaaaaaaata tgaacgccgg aatcaatgcg cgctcggcaa
     2401 gactctggca gtggaggcgc gccattaccg acactagcac gagacggtcg cccacggcgc
     2461 gccggaccag ttctgtcagc gcgtcgccag catattgcat atcggcatcg gcaaataata
     2521 aatagtccgg cggttcgggc aaagcttgcg cgtgcctgac gccctgatgc atcgcccaca
     2581 gcttaccggt ccatccatca ggaaggctcg gcgccgcaag cacggtcagt cggtcggatc
     2641 cgctggcggt tgcggcggcg tggcgagcga cggcggcggt cccgtcgctg ctgtggtcat
     2701 cgaccacaat aagcgtcaga ctgccgcgat aatcttgtcg cagcagtgac acgattgtat
     2761 tgtcgatgat gcttgcttcg tcacgggcgg gtatgacagc gaccacgcgc ggccaatgca
     2821 catcttgcga gaccgccggc tgtagcgcgg gctcactctg cgttgcacgc cagaaaccac
     2881 cgcgcgccgc aagcaaatag gcccagacaa ggcaacagag cgaagcgatg agcagcgctg
     2941 tcatcgcaga tatcccgctt cgcggaacca gtcgatagcg tcgtcaagtc catcagtgta
     3001 agggcgagca cggaatccca gttcacgctc tgccttggcg ctgctgaaga acatacgctg
     3061 cttggacatg cgcaagccct cgagggtcag caaaggctcg cgtccggtga tcgctgcaat
     3121 catctccgcc accgccgcga gcgggtagag cggtgcgcgt ggcagcttga atcgtggcgg
     3181 tcgttgtccc gcgagtcgcg cgatgtctcc gagcatgtcg gcaagcaaaa cgttctgtcc
     3241 gccaagtata tagcgttcgc cgatcttgcc gtgcgtgaac gcggcaacgt ggcccgccgc
     3301 cacgtcatcg acatgcacca gattgagtcc ggtgttaacg aacccgggga tgcgcccgga
     3361 ggccgcggcg acgatcagcc gccctgttgg cgtcggcctg acgtcgcgcg gaccgattgg
     3421 cgtcgaagga ttgacgatta tcgcgggcag cgcgtcgcgc gcaatcatgt cctcgaccag
     3481 tcgttcggcg acgaccttgc tgcgcttgta cgccccgacc tccaccgtgt cgggcagcgg
     3541 cgcggtctca tccgccggcg ctccgccgga acgcagcgtc agcgtggcga cggtactagt
     3601 gtacacaatg cgatcgacgc cagcggccag cgccgcaccc attaccgtcc gggtaccctc
     3661 tacattggtt cgcatgatcg ctttggaatc gcgtgcccat aggcgatagt cggcggcggc
     3721 atgcacgaca gagcttatcc cagacatggc acgcgtgatt gcgttcgcgt cccgaatgtc
     3781 tccctcgaca atctctatct cgagcccggc cagattgcca cgcgggctcg atgtgcgcac
     3841 aagcgcccgc accgggtacc ctgccccaag gaaggcacgg acaatcgccg atccgacaaa
     3901 accggtcgcc ccggtcacca gcacacgtcc gggctcgttc atcagaaatc ggaaatcatg
     3961 tatccgtcga tcggtgcaga taaatcaaca agccgcaggt tctcaagttt tatttttggt
     4021 ctctcaattg gattggccct cgttggcctt ctcttacttg ttttataaat ggtgcttaaa
     4081 ttctcgcaca agtatcgact ctcgaccaac gcgccgaagt tgaatggcaa gatcgccgcg
     4141 gaccgccgcg actctgtcac gtccagacga tcgtttgggt ggagaaacgt attgatcacc
     4201 agtatgtaaa ttagtcgatt ccgctaatat tgagggctac gcgatatcac agcgtaattc
     4261 cagcggcagg taacggcttc cgttacggtg cacgggtccc gaagcaatat ttggccctcg
     4321 cagtgaaacc agcgagagtc gtgc
//
LOCUS       DATASH010000288         2627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1028725,
            whole genome shotgun sequence.
ACCESSION   DATASH010000288 DATASH010000000
VERSION     DATASH010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028725"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VHV99_07995"
     CDS             complement(<1..435)
                     /locus_tag="VHV99_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX3635415.1"
                     /translation="MTIAFASTAANAAGRADPARRIEIRAFDGPVGAEVLGLDLSQPL
                     SQDDFVRVHRAHLDHHVLVFRDQRITPDQQIAFSRRFGPLQIHVLRQFQLPGYPEVLV
                     VSNIVENGQPIGLGDAGHFWHSDLSYKEKPSLGSMLHAQELPA"
     gene            778..1215
                     /locus_tag="VHV99_08000"
     CDS             778..1215
                     /locus_tag="VHV99_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018435611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX3635416.1"
                     /translation="MEQRLDFYKASPAAIKALVGVEERIGKSAIEKSLAELVRLRASQ
                     INGCAFCVDMHTTDARKGGESERRLATVVVWRETPFFTDRERAALEWTEALTLVSQEH
                     VPDAVWQAVRPHFSDEELVDLTLLVSAINAWYRFSIAFRKVPA"
     gene            1275..1964
                     /locus_tag="VHV99_08005"
     CDS             1275..1964
                     /locus_tag="VHV99_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX3635417.1"
                     /translation="MKIMHVDASAKRERSNSRALSRYFLERLRDEGVDIEVDYLDVTV
                     DTPPHVNEAFAIAIYTPPDERTPAMKATLAASDALCKRLLDADALLFAMPMYNWSMPS
                     AFKAFIDSITRTELTYVNAEDGHIEGQLARQKVLFITTRGADVRAGTPYAAMDALTPA
                     LKAAFGFLGVADPSFVDAQPLQFSDQAARLDALERARAELDAMAANWAHQMKPAAASE
                     MNDDAMALNQE"
     gene            1969..2400
                     /locus_tag="VHV99_08010"
     CDS             1969..2400
                     /locus_tag="VHV99_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX3635418.1"
                     /translation="MKIRRLIAALALAGLSIAGSAQTVFAAGTAATPETITPAFSEAI
                     ANVPGKTMTALVVDYAPGGKSRPHRHGQAFVVGYVLSGAIRSRVGHGEERVFHAGESW
                     TEKPGAHHTVSENASNTEPAKLLAIFVADTNDKNLVTFDKP"
BASE COUNT          536 a          827 c          796 g          468 t
ORIGIN      
        1 cgcgggcagt tcctgcgcgt gcagcatcga accgagactc ggcttctcct tgtacgacag
       61 gtcggaatgc cagaaatggc cggcgtcgcc gagaccgatc ggctggccgt tctcgacgat
      121 gttcgagacc accagcacct ccggatagcc cggcaactgg aactgacgca gcacgtggat
      181 ctgcagcgga ccgaagcggc ggctgaaggc gatctgctga tccggcgtga tgcgttggtc
      241 gcggaacacc agcacgtgat ggtccagatg cgcgcggtga acgcgcacga aatcgtcctg
      301 gctcagcggc tgactgagat cgagacccag gacttcggcg ccgacggggc cgtcgaacgc
      361 gcgaatctcg atgcgccggg ccgggtcggc acggccagca gcgttggcgg cggtggatgc
      421 aaaggcaatc gtcacgggaa actcttcgtc gaaactggtc ggaggacgcc gtcgaaggcg
      481 cagtcagacg aatttaccgg ctcgcgtgcg cgccgacaac gaagcaaatc cgatacacat
      541 atctgctgaa gtgataaacc cgatgtttta acgcgcagcg ggttttgggg cggcgaacac
      601 cttgtgtttt gacctgccgc gcgcgctgac ggcttgcgat ccggcatggc agggacatcc
      661 agaaaagtgg ccacccaaaa ttacacgaaa tcggtcattt aactgaacca gttgaaccgc
      721 taatctgcac ttaccccatc actgttgact gggccccaaa ccgtcacgga gtcaatcatg
      781 gaacaacgtc tcgacttcta caaagccagc cccgccgcca tcaaagcgct agtcggcgtc
      841 gaagaacgca tcggcaaatc ggccatcgag aaatcgctgg ctgaactggt ccgtctgcgc
      901 gcctcccaga tcaacggctg tgctttctgc gtcgacatgc acaccaccga cgcccgcaaa
      961 ggcggcgaat ccgagcggcg tctggccacc gtcgtggtgt ggcgtgaaac gcccttcttc
     1021 accgaccgtg aacgcgccgc gctcgaatgg accgaagcgc tcacgctggt gtcgcaggaa
     1081 cacgtacccg acgccgtatg gcaagccgtg cgcccgcatt tcagcgacga ggaactggtc
     1141 gacctgacgc tcctcgtctc cgccatcaac gcgtggtacc gcttttcgat cgcgtttcgc
     1201 aaagtgccgg cttgagggcg ccgccgccgt gcccgcgcga gccatttccg cctgaaccct
     1261 gaaggaaaga agtcatgaaa atcatgcacg tcgatgcgag tgccaaacgc gagcgatcca
     1321 actcgcgcgc gttgagccgg tattttctgg agcgcctgcg tgacgaaggc gtggatatcg
     1381 aggtcgatta cctcgacgtc acggtcgaca cgccgccgca cgtgaatgag gcattcgcca
     1441 ttgcgatcta tacgccgcca gatgagcgca ctcctgccat gaaggccacg ttggcggcat
     1501 cggatgcgct gtgcaagcgc ctgctggacg ccgacgcatt gctctttgcg atgcccatgt
     1561 acaactggtc gatgccctcc gcgttcaagg ctttcatcga cagcatcacg cgcacggagc
     1621 tgacctacgt gaatgcggaa gacggacaca tagaaggcca gcttgcccgg caaaaggtgc
     1681 ttttcatcac gactcgcggc gcggacgtac gcgccggcac gccctacgcg gcgatggatg
     1741 cgctgacgcc cgcgctgaag gccgctttcg gatttctcgg tgtggcggat ccgtctttcg
     1801 tcgacgctca gccgttgcag ttttcagacc aggcggcgcg gctcgatgcg ctcgaacgcg
     1861 cgcgcgccga gctcgacgcg atggcggcga actgggccca ccagatgaag ccggccgcgg
     1921 cgtccgaaat gaacgacgat gccatggcat tgaaccagga gtaatcgcat gaaaattcgt
     1981 agactaatcg cggcgctagc gcttgccggt ctttccatcg cgggaagcgc ccaaaccgta
     2041 tttgccgccg gtacggccgc taccccggag acgattacgc ccgcgttcag cgaggcaatc
     2101 gccaacgtgc cgggcaaaac aatgacggcg ctcgtcgtcg actatgcccc tggcggcaag
     2161 tcgcgaccgc atcggcatgg acaggctttt gtggtcggct atgtgctctc gggcgcgatc
     2221 cgcagccggg ttggtcatgg cgaggagcgc gtctttcatg cgggcgaatc ctggaccgag
     2281 aagcccggcg cgcatcacac ggtcagcgaa aacgcgagca atacggaacc cgcgaaacta
     2341 ctggccattt tcgtggcgga cacgaacgat aaaaatctcg tcacgttcga caaaccatga
     2401 gcgcatgacg tgagaggcat gagggtccac gcgcttgccc gcatggacct ggctgtgcac
     2461 ccggccatcg agcctgagcc ctagaacttt ggataacact cgaaattcac tgacgctgcg
     2521 tgctccgtca tcatctgcac gccgctcacc gtttcagtcg tgacactgct ctgcatgccg
     2581 cgacgctcgc aatagtcccg cgcctcgctg attgcgtgtt catgcgc
//
LOCUS       DATASH010000289        12997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3401583,
            whole genome shotgun sequence.
ACCESSION   DATASH010000289 DATASH010000000
VERSION     DATASH010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 12997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12997
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3401583"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1061
                     /locus_tag="VHV99_08015"
     CDS             <1..1061
                     /locus_tag="VHV99_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3635419.1"
                     /translation="NAVAGADQARSTAINRIVGGDSPTAAQFNTSRQFHDLIDQGLLN
                     NVDDVAAKENWNSVFPQSILESIKVKGHYYAAPVDIHMPAWFFYSKPVFQKAGIAAEP
                     KSYDEFIADLGKLKTAGVIPLALGGQPWQEKITFDAVFADVGGPDLYLKVYRDRDQNA
                     VKSDAFKKVLASFKRLHDFVDPGSPGRNWNDATALVISGKAGVQIMGDWAKGEFSAAN
                     QTAGKDFGCFPGFGPHSPYLVAGDVFVFPKTDNANAIKAQNLLATVMTSPAAQVAFSA
                     KKGSIPIRPDVDASSLDICAKEGMAIMKDKSRQLPNPEMLLSPDTQGALIDVITNFWN
                     KNQSVDDAQKAFASALKS"
     gene            1071..2012
                     /locus_tag="VHV99_08020"
     CDS             1071..2012
                     /locus_tag="VHV99_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX3635420.1"
                     /translation="MHALKLPAAGSRSRKSPLKKPLKKRFPFAVWLALLPMVLTVVFA
                     YLGTMVWTARVSLSNSRTFPSGDFAGLTQYTRLFNNDRWLLSLQNIVTYGACFIVACM
                     VIGLLLAIFIDQRVVAEGALRTVFLYPYAMSFVATGLVWQWILNPQLGAQAVLHKLGF
                     AHARFDWIVDQDWVIYTIVIATVWQASGLVMALLLAGLRGIDDELWKAARIDGIPRWR
                     VYASIVVPMLGPSLSTAFVLLFVMVVKLYDAVVAMTQGGPGTASEVPAKFIMDYLFGR
                     ANIGLASAASVVLLATVLAILAPFFYARSRAALKKEV"
     gene            2012..2923
                     /locus_tag="VHV99_08025"
     CDS             2012..2923
                     /locus_tag="VHV99_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX3635421.1"
                     /translation="MNTLSPPLKGGTPSRTQRRRRAFTPARLGVYAFLLTAALFFLLP
                     LYVMLVTSIKPMSEIRLGNLLALPTHFTLDAWSAAWQSACTGLDCNGIQVGFWNSVRI
                     VVPSTVLSIVIGAVNGYALSFWRPRGAGLLFGVLLMGAFIPVQVMVYPLVRVLASVRL
                     FSSLPGIVLIHTIFGMPVMTLLFRNYYASIPQELFKAARIDGGGFWRIFLQLMLPMST
                     PIIVVAVIMQVTGIWNDFILGLVFAGTKNLPMTVQLNNIINTTTGERLYNVNMAATIL
                     TSMVPLAVYFISGRWFVRGIASGAVKG"
     gene            2938..4146
                     /locus_tag="VHV99_08030"
     CDS             2938..4146
                     /locus_tag="VHV99_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3635422.1"
                     /translation="MTNLANVANAAANPAANAAGAVNVGDAANLGNVADAAGLSNPAN
                     VSVRNLKIQLGATTVIENLDLDVQAGEFVVLLGPSGCGKSTLLHSIAGLIDVTDGSIE
                     IAGEDMTWADPKDRRIALVFQSYALYPTMSVERNLSFALRINGTPKAEIERRVTRASE
                     MLQLGPLLKRKPAQLSGGQRQRVAIGRAIVREADVFLFDEPLSNLDAKLRTELRRELK
                     QLHQRLGATMIYVTHDQVEAMTLATRMAVMRGGVIQQFGTPAEVYARPNNLFVATFLG
                     SPAMNVLKGTLEAREGSLYFCTAHLRFDVSPYPFRNAPADRTPCLLGVRAEDVHVGEG
                     SSPTMTGRATISLVEPMGNHRVVWLDYHGVQIASIDQTKTPVAIGDAATFSFDGAHIS
                     LFDEEGGARL"
     gene            4198..5259
                     /locus_tag="VHV99_08035"
     CDS             4198..5259
                     /locus_tag="VHV99_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX3635423.1"
                     /translation="MATLKDVAALAGVGMSTASRAISGKGPISADAAARVKAAIEALN
                     FRPSSIGRAMATQSLGMIGIFVPTFFGSYYGTILKQTDTELRAVHRHVVVATGCGEVS
                     PREQAMEAVRFLIGRDCDGVVVISHDLHDDDLDMLHRMHPKMVFLNRAFDQLPEASFC
                     ADHRRGGELAARTLLDHGHRDIAVISGPFSASDNQIRLEGFFAELSREGIAREDVTLI
                     ESDFSPEGGYAAAQKLLDSKRRFTGLFCANDTMAVSVLARFHQAGIAVPDEVSVIGYD
                     DDYSAAYAAPGLTSVHIPTAELTQNAVRWLVNQCYRTSWEIFREFPVSVTMRESVGPA
                     PGAAASSSRIKTASCSSAS"
     gene            complement(5347..6204)
                     /locus_tag="VHV99_08040"
     CDS             complement(5347..6204)
                     /locus_tag="VHV99_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA4780 family RIO1-like protein kinase"
                     /protein_id="HEX3635424.1"
                     /translation="MKTPKRLLPLVEEGLIDEVISQLMSGKEATVYVVRSGESTRCAK
                     VYKDAKQRSFRQAASYREGRKVKNSRQQRAMEKGSRYGREVQEQAWQNAEVDALFQLA
                     NAGVRVPQPFICTDGVLLMELVTDADGNVAPRLNDVEMTEARALELHALLLNQVVRML
                     CAGMIHGDLSEYNILLAADGPVIIDLPQAVDAAGNLEAPAMLERDVNNLATYFGRFAP
                     ALLDSSYGKEIWSLYEAGALHVDAELTGRVELDTTPIDLESVLQELEDTRLDEEARLR
                     YEQSLRSGT"
     gene            complement(6397..7653)
                     /locus_tag="VHV99_08045"
     CDS             complement(6397..7653)
                     /locus_tag="VHV99_08045"
                     /inference="COORDINATES: protein motif:HMM:NF019421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HEX3635425.1"
                     /translation="MVSNKPRDAFKVFLDAAELNSQQQVGTLYRQTARTDLPASFAYA
                     RSWVDGRNAFMLDPRLELWTDEQYPPARSAAFGVFMDSAPDRWGRVLMERREAAAADR
                     EDRPMRNLQEIDFLLGVHDLTRVGALRFQNVEGHFLDNSANAAPPVTDLETLAYISLR
                     IEEADIEKLPEYEQWLAMLIAPGTSLGGARPKANFTSFGDDLWIAKFPAKDDRYDIGA
                     WEYIVHRLARNAGIWVPQSELRYVGARYGTFCVERFDRREGSRRMYTSAMTLLERQDG
                     ESDASYLDIAEYLAGNGAQGHIEGDLAQLFRRVVFNVLVGNRDDHLRNHGFIREASGW
                     RLSPAFDMNPNPSKREHALTLDGVSALPSLETALETAEFYRIDEGQARRIVAEVQNAV
                     KPWQQEAAALNLSRPEIQRMENVFQA"
     gene            complement(7640..7975)
                     /locus_tag="VHV99_08050"
     CDS             complement(7640..7975)
                     /locus_tag="VHV99_08050"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX3635426.1"
                     /translation="MRALGERLKLARLRRELPTVLFAERLGISRDTLNRLEKGDPNIA
                     LGTYVRALRVLGLDKDMDAIARDDELGRKLQDLKLPGQRKPRTNPSGVALIKAERPAR
                     KTKKTDGQQ"
     gene            8571..10283
                     /locus_tag="VHV99_08055"
     CDS             8571..10283
                     /locus_tag="VHV99_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEX3635427.1"
                     /translation="MRDFLANITTRLGVLKGVLPLSRAAAARDALAGVQLASMDIPQV
                     LGYARIAGMPAVTGLYTVFLPLIAFAFFGASRHLVVAADSATATIFASRLSTMAPASS
                     ADYAALAGMVALLTAALLLIARIFKLGFLADFLSRTVLVGFLAGVGVQVGIAMLGDML
                     GLSGHAASSIAQLALVARDVARINLPTLAISALVVVSILVCKRFLPRVPVPLIAVVAG
                     IAASDIYGFAAHGISVLGPVAGGLPPLRMPSVTWQQVLDLLPVAASCFVMIVAQSAAA
                     SRVFAERYHETVDTNADLLGIAAANAAAALSGAFVVNGSPTQTAMADRAGTRSQFAQI
                     VFAAVVVVVLLFFSRFLQYLPHCILASIVFTIAVGLINLKTLFSIRRESPGEFALAVF
                     TAAAVVLIGVEHGILVAVALSLLRHVRHSYRPHTMILVPGEDGIWVPVPAAPGLQTAP
                     GLIVYRFGADLFYANDHFFVDDARRLIDQAPSAVRWFVIDASAITDLDYSAARSVGEL
                     CEMLKHSGVEVMFARVNRYLRSDMDRHGITPVVETRCIFSTLHEALRMAGVEQPGAHV
                     KDPL"
     gene            complement(10321..10680)
                     /locus_tag="VHV99_08060"
     CDS             complement(10321..10680)
                     /locus_tag="VHV99_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635428.1"
                     /translation="MSGSNDLNPAAKRSPLQRRLYLAGAVVLVAGLIAAAIIYAMAPA
                     PDSAVSMYSIADPRYQIELQRIGGNAAVVMAQLHQWFDGLWHGTALAYTVAVLGAMLA
                     GACFFIAYFFADEEPQS"
     gene            complement(10750..11313)
                     /locus_tag="VHV99_08065"
                     /pseudo
     CDS             complement(10750..11313)
                     /locus_tag="VHV99_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918386.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
     gene            complement(11347..12231)
                     /locus_tag="VHV99_08070"
     CDS             complement(11347..12231)
                     /locus_tag="VHV99_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3635429.1"
                     /translation="MLNELKTFIAVSQSGTFSGAGARIGLTQSAVSAQMQRLEEELGF
                     ALFDRTGRSATLNDAGRETLTLAEEMMTLYSRLSERGAGAAESGMLRVGAIASAQVSF
                     LVDALARFRDARPGWRIRVVPGVSLGLLGQVDAGELDLAVIIKPPFALPSELEWRTLV
                     TEPFVLLVPKALARGKRSWRELLRSEPFIRYDRGSFGGRLVDRFLRRARLNVQDVIEL
                     DELQGIAQLVARGIGVALIPNTAGLGKWPASVTALELGDATFHREIGLVQRPRHSRQA
                     VAGALADCIGIAAGAGGG"
     gene            12399..12833
                     /locus_tag="VHV99_08075"
     CDS             12399..12833
                     /locus_tag="VHV99_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3635430.1"
                     /translation="MSVAETVLPPFHLAFPVHSIAAAREFYGELLGCPEGRSAPDWVD
                     FNFYGHQIVAHLAPEETGHRQTSKVDGDAVPVRHFGAVLSMEQWQSAADKLQKAGIDF
                     IIEPHVRFKGEVGEQATMFFLDQSGNALEFKAFANMSSLFAK"
BASE COUNT         2291 a         3938 c         4101 g         2667 t
ORIGIN      
        1 acaacgcggt ggccggcgcc gatcaggcgc gctccaccgc gatcaatcgg atcgtcggcg
       61 gcgattcacc caccgctgcg cagttcaata cgtccaggca gtttcacgac ctgatcgacc
      121 agggcctgct gaacaacgtc gacgacgttg ccgcgaaaga aaactggaac agcgtgttcc
      181 cgcagtcgat cctcgagagc atcaaggtga agggccatta ctacgccgcg ccggtcgata
      241 ttcatatgcc ggcctggttc ttctattcaa agccggtgtt ccagaaggcc ggcattgccg
      301 ccgagccgaa gagctacgat gaattcatcg ccgatctcgg caaattgaaa accgcgggtg
      361 tgatcccgct cgcgctgggc ggtcagccct ggcaggaaaa gatcacgttc gacgcggtat
      421 tcgccgacgt cggcggtccc gatctctatc tgaaggtgta ccgcgatcgc gaccagaacg
      481 cggtcaagtc cgacgccttc aagaaagtcc tcgcgtcgtt caagcgcttg catgacttcg
      541 tcgatccggg ctcgcccggc cgcaactgga acgatgccac cgcgctggtg atttccggca
      601 aggccggtgt gcagatcatg ggcgactggg ccaagggcga attctcggcg gccaaccaga
      661 ctgccggcaa ggacttcggc tgcttcccgg gtttcggtcc gcactcaccg tatctggtcg
      721 cgggcgacgt attcgtgttt ccgaaaaccg ataacgccaa cgcgatcaag gcgcaaaatc
      781 tgcttgccac ggtgatgacc tcgccggccg cgcaagtcgc attcagcgcg aagaaaggct
      841 cgattccgat tcgtcccgac gtggacgcca gcagtctcga catctgcgcg aaggagggca
      901 tggcgatcat gaaggacaag tcgcgtcagt tgccgaatcc ggaaatgctg ctctcgcctg
      961 acacgcaggg cgcgttgatc gacgtcatca cgaacttctg gaacaagaac cagtcggtcg
     1021 acgatgcgca gaaggcgttt gccagtgcgt tgaagagcta ggcgctcgcc atgcacgcgc
     1081 tcaagctgcc agccgccggc tccaggtctc gcaaaagccc gctgaaaaag ccgctgaaaa
     1141 agcgttttcc gtttgcggtg tggctggcct tgctgccgat ggtcctgacc gtcgtgttcg
     1201 cgtatctcgg cacgatggtc tggaccgcgc gcgtgtccct cagcaactcg cgcacgtttc
     1261 cttccggcga cttcgcgggc ctcacacaat acacgcggct tttcaacaac gaccgctggt
     1321 tgctgtcgct gcaaaacatc gtgacttacg gtgcatgttt cattgtggcg tgcatggtga
     1381 tcggtctgtt gctggcgatt tttatcgacc agcgcgtggt ggcggaaggc gcgttgcgca
     1441 cggtgtttct ctatccgtat gcgatgtcgt ttgtcgcaac gggtctggtc tggcagtgga
     1501 ttctcaatcc gcaactgggc gcgcaggccg tgttgcacaa actcggcttc gcgcatgcgc
     1561 gtttcgactg gatcgtcgat caggactggg tgatctacac catcgtcatc gcgacggtat
     1621 ggcaggcgtc cggcctcgtg atggcgctgc tgctggccgg cctgcgcggg atcgacgacg
     1681 agctgtggaa agccgcgcgt atcgacggga ttccgcgttg gcgcgtctat gcgagcatcg
     1741 tcgtgccgat gctcggaccg tccctctcca cagccttcgt gctgctcttc gtgatggtcg
     1801 taaagcttta cgacgccgtc gtcgccatga cgcaaggcgg ccccggcacg gcgagcgaag
     1861 taccggcgaa gttcatcatg gattatctgt tcggtcgcgc gaacatcggc ctcgcgtcgg
     1921 cggcgtcggt cgtgttgctg gccacggtgc tcgcgattct cgcgccattc ttctatgccc
     1981 gcagccgcgc tgcgttgaag aaggaggtgt gatgaacacg ctcagtccgc cactgaaggg
     2041 cggcacgccg agccgtacgc agcggcgccg ccgggctttt acacccgcgc gcctcggcgt
     2101 ctacgcgttc ctcctcaccg ccgcgttgtt cttcctgttg ccgctgtacg tgatgctcgt
     2161 cacctcgatc aaaccgatga gcgagattcg actcggcaat ctgctcgctt tgcccacgca
     2221 tttcacactc gacgcatgga gtgccgcgtg gcagtccgcg tgtaccgggc tcgattgcaa
     2281 cggcatccag gtcggcttct ggaattcagt gcggatcgtc gtgccgagca cggtgttgtc
     2341 gatcgtgatc ggcgcggtga acggctatgc gctgtccttc tggcggccgc gcggggccgg
     2401 gctgctgttc ggcgtgctgc tgatgggcgc cttcattccc gtgcaggtga tggtctatcc
     2461 gctcgtacgc gtgcttgcga gcgtgcgtct gttcagttcg ctgccgggca tcgtgttgat
     2521 tcacaccatc ttcggtatgc cggtgatgac gctgctgttc cgcaactact acgcgtcgat
     2581 tccgcaggag ttgttcaagg cggcgcgcat cgacggcggc ggcttctggc gcatcttttt
     2641 gcaactcatg ctgccgatgt ccacgccgat catcgttgtc gcggtcatca tgcaggtgac
     2701 aggcatctgg aacgacttca ttctcggcct cgtgttcgcc ggcacgaaaa atctgccgat
     2761 gacggtgcaa ctgaataaca ttatcaacac gacgaccggc gagaggctct ataacgtcaa
     2821 tatggcggcg accattctga cctccatggt gccgctagcg gtctacttca tttcgggccg
     2881 ctggtttgtg cgcggcattg cgtccggcgc cgtcaaggga taagtcaagg atcgggcatg
     2941 acaaacctgg ctaatgtggc aaatgcggcg gcaaatccgg cggcaaacgc agcgggcgcg
     3001 gttaatgtag gagacgcggc aaacctcggc aacgtggcgg atgcggcggg tctatcgaat
     3061 ccggccaatg tctcggtacg caatctgaag atccagctcg gcgcgactac ggtgatcgag
     3121 aacctggatc tcgacgtgca ggcaggcgag ttcgtcgtgc tgctcggccc atcgggttgc
     3181 ggcaaatcca cgttgctgca cagtatcgcg ggtttgatcg acgtgaccga cggcagcatc
     3241 gagattgccg gcgaagacat gacctgggcc gacccgaaag accgccgcat tgcgctggtg
     3301 tttcagtcgt acgcgttgta tccgaccatg agcgtcgagc gtaatctgtc gtttgccttg
     3361 cgcatcaacg gcacgccgaa ggcggaaatc gagcggcgcg tcacgcgggc gtccgagatg
     3421 ttgcaactcg gcccgctgct caaacgcaag ccggcgcaat tatcgggcgg ccagcggcag
     3481 cgcgtggcga tcggccgcgc gattgtgcgc gaagccgacg tgtttctatt cgacgagccg
     3541 ctgtccaatc tcgacgccaa attgcgcacc gaattgcgcc gcgagctcaa gcaactgcac
     3601 cagcgtctgg gcgcgacgat gatctacgtg acccacgatc aggtcgaagc gatgacgctc
     3661 gccacgcgca tggccgtgat gcgcggcggc gtgatccagc agttcggcac gcccgccgaa
     3721 gtctatgcgc ggccgaacaa tctgttcgtc gcgactttcc tgggctcgcc cgcgatgaat
     3781 gtactcaagg gcacgctgga agcacgcgag ggctcgctat acttttgtac ggcgcatttg
     3841 cgattcgacg tatcgccata cccgtttaga aacgctcccg cggatcgtac gccttgcctg
     3901 ctcggcgtac gggccgaaga cgtgcacgtg ggcgagggtt cgagcccgac aatgaccggg
     3961 cgcgcgacca tttcactggt cgagccgatg ggcaaccacc gcgtcgtctg gctcgactat
     4021 catggcgtgc aaatcgcgtc gatcgaccag acgaaaacgc cggtggcgat aggggatgcc
     4081 gcgacgttct cgttcgatgg cgcacatatt tcgctgttcg acgaagaagg cggcgcgcgt
     4141 ctatagtgat cgcatgaagc gtcgttgaat acaacaaaag gcttgacgga gaaccgcgtg
     4201 gcaacactca aagatgtcgc ggcactggcc ggcgtcggca tgtcgaccgc gtcgcgagcc
     4261 atttccggca aggggcccat ttcagccgat gccgccgcgc gcgtaaaggc ggcgatcgaa
     4321 gcgctgaatt tccggccgtc gtccattggc cgggccatgg caacgcaatc gcttggcatg
     4381 atcggcattt tcgtgccgac cttcttcggc tcctactacg gcacgattct caagcagacc
     4441 gacaccgaac tgcgcgcagt gcaccgccac gtggtggtag cgacgggctg cggggaagtg
     4501 tcgccgcgcg agcaggccat ggaggcagtg cgttttctga tcggccgcga ttgcgacggc
     4561 gtggtggtga tcagccacga tctgcacgac gacgatctgg acatgctgca tcgcatgcat
     4621 ccgaagatgg tatttctgaa tcgcgcgttc gatcagttgc ccgaggcgtc gttctgcgcg
     4681 gatcaccggc gcggcggaga actggctgca cgcacgctgc tcgatcacgg gcatcgggat
     4741 atcgcggtga tttccgggcc gttcagcgcc tccgacaatc agatccggct cgaaggcttc
     4801 tttgccgaac tgtcgcgcga gggcatcgcc cgtgaggacg tcacgctgat cgaatcggac
     4861 ttctcgccgg aaggcggcta cgcggctgcg cagaaactgc tcgattcgaa gcggcgcttc
     4921 acgggactct tctgcgcgaa cgacacgatg gcggtgagtg tgctggcgcg cttccaccag
     4981 gcgggcattg ccgtaccgga cgaagtctcg gtgatcggct atgacgatga ttattcagcc
     5041 gcctacgccg cgcctgggct cacctctgta cacatcccga cggcggagtt gacgcagaac
     5101 gcggtgcgct ggctggtcaa tcagtgctac cggacctcgt gggagatctt ccgcgagttt
     5161 ccggtgagcg tgacgatgcg ggaatcggtg ggaccggcac ctggggctgc cgcttcatcg
     5221 agcaggatca agacggccag ttgcagtagc gcttcgtagt ttcttcccga agcggcgtgc
     5281 aggtgcgtgc aactacgacg ccgctcatga accgctgatg cgttgcgccg ggacggctca
     5341 gagtggtcag gtcccgctgc gcagcgactg ttcgtagcgc aggcgcgctt cttcgtcgag
     5401 ccgcgtgtct tcgagttcct gtagcacgct ttcgagatcg atcggcgtgg tgtccagttc
     5461 gacccggccg gtcaattcgg catccacgtg caacgcgccc gcttcgtata gcgaccagat
     5521 ttccttgccg tagctcgagt cgagcaaggc gggcgcgaag cggccgaaat aggtggcgag
     5581 gttgttcacg tcgcgctcca gcatggcggg cgcttccaga ttgccggcgg cgtccacagc
     5641 ttggggcagg tcgatgatca ccgggccgtc cgctgcgagc aggatgttgt attcggacag
     5701 gtcgccgtga atcatgccgg cgcacagcat gcgcaccacc tggttgagca gtagcgcgtg
     5761 caattcgagc gcgcgcgctt cggtcatctc gacatcgttc agacgcggtg cgacgttgcc
     5821 gtccgcatcg gtcaccaatt ccatcagcaa tacgccgtcg gtgcagatga agggctgcgg
     5881 cacgcgcacg ccggcgttgg cgagctggaa cagcgcatcc acttccgcgt tctgccacgc
     5941 ctgttcctgc acttcgcggc catagcggct gcctttttcc atcgcgcgct gctggcggct
     6001 gttcttgacc ttgcggcctt cgcgatagga cgcggcctga cggaaactgc gttgtttggc
     6061 gtccttatag accttcgcgc agcgggtgga ttcgccgctt cggaccacgt acacggtggc
     6121 ttccttgccg ctcatcaact gcgagatgac ttcgtcgatc aggccttctt cgaccagcgg
     6181 cagcagacgt ttgggtgttt tcatgcggcc gccgtggggt ggagggtggc gcggcggcca
     6241 tgggtggccg catgggtggc cgcgttctgg agcgtaggga gggaaattcg gatcatgccg
     6301 gattatacgg ggtgagaggc caggtaccgg aacgccggcg tgaagttgcc ggttgtgacc
     6361 ggcacgcctc gtacacttcg ctgccaatcg aagccatcaa gcctgaaaga cgttctccat
     6421 ccgctggatt tcgggccggg acagattgag cgcggcggct tcttgctgcc atggtttgac
     6481 ggcgttttga acttccgcga cgatgcgacg cgcttgcccc tcgtcgattc gataaaactc
     6541 ggctgtttcg agcgcggttt caagactcgg cagggcgctg acaccgtcca gcgtgagcgc
     6601 gtgttcccgc ttgctcgggt ttggattcat gtcgaacgcc ggcgagagcc gccagccgga
     6661 agcctcgcga atgaacccgt gattgcgaag atgatcgtcc cggtttccca cgagtacgtt
     6721 gaagacgacc cttctgaaga gctgagcgag atcgccctcg atatgacctt gtgcgccgtt
     6781 gccggcaaga tactcggcaa tgtccagata gcttgcgtcc gattcgccgt cctgacgttc
     6841 gagcaaggtc atggcggagg tgtacatgcg ccggctgcct tcacggcgat cgaatcgctc
     6901 gacgcagaac gtgccgtagc gcgcaccgac gtacctgagt tcggactgcg gcacccagat
     6961 gccggcgttc ctcgccaacc gatgcacgat gtattcccat gcgcctatgt cgtaacggtc
     7021 gtctttcgcg gggaatttgg cgatccagag atcgtcgcca aaactagtga agttggcttt
     7081 cgggcgagcg ccgccgagcg acgtgccagg ggcgatcagc atggcgagcc actgctcgta
     7141 ttcgggcagc ttttcgatat ctgcttcctc gatgcgcagg ctgatatacg ccagggtttc
     7201 gaggtcggtg accggcggcg cggcgttggc gctgttgtcg agaaaatgac cttccacgtt
     7261 ctgaaaccgc agtgcgccga ctctcgtcag atcgtggacg ccgagcagga aatcgatctc
     7321 ctgcaaatta cgcatgggcc ggtcttcgcg atccgcggcc gcggcttcgc ggcgctccat
     7381 caggacgcgg ccccagcgat ccggcgctga gtccatgaag acgccgaatg cggcggacct
     7441 cgcaggcggg tattgctcgt cggtccataa ctcgaggcgc ggatcgagca tgaatgcatt
     7501 gcgcccgtct acccagcttc tcgcataagc aaacgatgca ggcaagtcgg tgcgtgcagt
     7561 ctggcgatac aaggtgccga cctgttgctg gctgtttaat tcggctgcgt cgagaaatac
     7621 cttgaacgca tctcgcggtt tattgctgac catcggtttt cttcgttttt cttgccggcc
     7681 gttctgcttt gatgagagcg acgccggacg ggttcgtgcg tggttttcgc tgccccggca
     7741 acttgagatc ctgcagcttg cgcccgagtt cgtcgtcgcg ggcgatggcg tccatgtcct
     7801 tgtcgagccc caacacgcga agcgcacgca cgtaggtgcc gagcgcgata ttcggatcgc
     7861 ccttctcaag gcggttcaac gtatcgcgcg aaatgcccag gcgctccgcg aacaggacgg
     7921 ttgggagttc gcgccgcagg cgggcgagtt tcagccgttc gccgagagca cgcatctgct
     7981 tctcgacgga cggaaaggga cgctgaaagg tgcgaggcat gtccgataga ttaggcaaat
     8041 attgaaatta tgtcaaagat atcggacaaa gcactggatc tggcactcga aaagcgttgt
     8101 cgacggtaca taaattaaac tggtgtgtcc gatactttag gcagagtatc aaattttgaa
     8161 tgaagtatcg gacatattga agcatgcaaa tagccctgcg cgcaggcctt gcgttagtgt
     8221 cgggccgtaa tgcgccgcaa cagtgttttg cgtttttcct cattcatttc gcataccaat
     8281 cgcttagact tgcccgtaac aatttgttac ggggtgtgag atgttggccg tatttatcat
     8341 tgcctgtccg atctgcatcg ctgcgttgtt cggttttgcg cggcacgtgg atccgctgac
     8401 cgggcagttc aagcgttaaa tcaaaataga acgattcggg ttcagctacg ccgcggggat
     8461 gactggcagg tcctggcgat cttcttcagc tgggcgcgcg ctcagccttg cctttatgtg
     8521 ccgagtcgcg gcacgatgcg cgataaccta agttttgggc agcatcgcgc atgcgagatt
     8581 ttctggcaaa catcacgacg cgccttggcg tactgaaagg cgtgcttcct ttaagccgtg
     8641 ccgcggcggc gcgcgacgcg ctggccggcg tgcaactggc gtcgatggat attccgcaag
     8701 tgctcggcta tgcgcgcatt gcgggcatgc ctgccgttac ggggctttac accgtctttc
     8761 tgccgctcat cgcgttcgcg ttcttcggcg catcgcggca cctcgtggtg gctgccgact
     8821 ccgccaccgc gaccattttc gcgagccgcc tctcgaccat ggcgccggct tcgagcgccg
     8881 actatgcggc gctcgccggg atggtcgcct tgctcaccgc cgcgcttctc ctcatcgcgc
     8941 ggatcttcaa actcggcttt cttgctgact ttctctcgcg cacagtgctg gtcggctttc
     9001 tcgccggcgt cggcgtccag gtcggcatcg ccatgctggg cgacatgctc ggcttgtccg
     9061 ggcatgccgc gagcagcatc gcccaactcg cgctggtggc gcgcgatgtc gcgcggatca
     9121 acctgccgac gctcgctatt tccgcgctgg tcgtggtctc cattctggtt tgcaagcgct
     9181 ttttgccgcg cgtgccggtg cccctgatcg cggtggtggc gggcattgca gcgagcgata
     9241 tctacggctt cgccgcgcac ggcatttctg tactcggccc ggtggcgggc ggcctgccgc
     9301 cgctgcgcat gccgtcggtc acgtggcagc aggtgctcga tctgctgccg gtcgccgcct
     9361 cgtgtttcgt gatgatcgtt gcgcaaagcg ccgccgcgag ccgcgtattc gccgagcgtt
     9421 accacgaaac cgtcgatacc aacgccgatc tgttggggat tgccgccgcc aacgccgcgg
     9481 cggcgttgtc gggcgcgttc gtggtgaacg gcagtccgac gcaaaccgcc atggccgatc
     9541 gcgcgggtac gcgaagccag ttcgcgcaga tcgtattcgc ggcggtggtg gttgtagtgt
     9601 tgctgttttt cagccgattt ttgcagtacc tgccgcactg cattctggcc agcatcgtgt
     9661 ttacgatcgc cgtcggcctg atcaatctga aaacgctgtt ttcgattcgc cgcgaaagtc
     9721 ccggtgaatt cgcgctggcc gtgttcaccg cggcggccgt ggtgttgatc ggtgtcgagc
     9781 acggcattct ggtggcggtg gcgttatcgc tgctgcgaca tgtgcggcac agttaccggc
     9841 cgcacaccat gattctcgtg cccggcgaag acggcatctg ggtgccggtg ccggccgcgc
     9901 ccggacttca gaccgcgccg ggcctgatcg tctatcgctt cggcgccgat ctgttctatg
     9961 cgaacgacca ctttttcgtc gacgacgccc gccgtctgat cgatcaggcg ccgagcgcgg
    10021 tgcgctggtt cgtgatcgac gcgagcgcga ttaccgacct cgactattcg gcggcgcgtt
    10081 cggtcggcga gttgtgcgag atgctcaagc acagcggcgt cgaagtgatg ttcgcgcgcg
    10141 taaaccgcta tttacgctcg gatatggacc ggcatggcat cacgccggtc gtcgaaacaa
    10201 gatgtatttt cagcaccctg catgaggcgc tgcgcatggc aggcgtggag cagcccggtg
    10261 cgcatgtcaa agacccgttg taactgcctg tttagcgggg ggggggcgtg gcggatggct
    10321 tcaggactgc ggctcttcgt cggcaaagaa gtaggcgata aaaaaacatg cgccggcaag
    10381 catggcgcca agcacggcca cggtataggc gagcgccgtt ccgtgccata gaccgtcgaa
    10441 ccactgatga agctgcgcca tcaccactgc cgcgttgccg ccgatgcgct gcaattcgat
    10501 ctgatagcgc gggtccgcga tgctgtacat ggagacggcg ctgtccggcg ccggcgccat
    10561 cgcatagatg atcgccgcgg cgatcaggcc ggcgaccagc acgacggcgc ccgccagata
    10621 aagccgtctt tgtagtggcg agcgctttgc tgccggattc aggtcattgc taccgctcat
    10681 cgtgttcgcg tgtccgctcg agtcatcagg ccgacagtat cgcagagcgg tcgcgtccct
    10741 aaggcacgcc tagcttttcg acgtcagcag tttgatcccg gcgatgccga acatgatcgc
    10801 gaacgagctg tccagccagc gacggatcgc gacgtagatg cgccgtgcgg acgcggtcga
    10861 aaacagcact gcgtagctgc tgaacacgga tacgccgatc accatgcagc caagcaccac
    10921 tggcgcggtg tccgacgtgc cgctcgccga cgacatggcc agcgaaacga tcgagagcca
    10981 caccagaatc gctttcggat tggtgagatg cagcagcagg ccgcgcaggt agagccgctt
    11041 gagcggctcg ctttcgcgtg cggtttgggc gggcagcttt tccgcgctga atgcggagcg
    11101 cgcggatttg aagcctggcc acagcagata aagtccgccg gcaatcctga tcgccaccag
    11161 acattccgag taagtggcga gtaccgccga caagcccagc gatgccagta acgcccagaa
    11221 aaacgggccg gacaccacgc cgagcgcgaa ggtgagcgcg gatttccggc cggcactcat
    11281 ggcgagcgac atgatcgcga gattgctcgg cccgttcggc tcgacaactc aggtcaacca
    11341 ctcaactcag ccgcctcccg ctcccgccgc gataccgatg caatccgcca gcgcacccgc
    11401 caccgcctgg cggctatgcc gcgggcgctg cacgaggccg atttcacggt gaaacgtcgc
    11461 gtcgccgagt tcgagcgcgg tgacgctggc gggccacttg ccgagtcccg ccgtattcgg
    11521 aatgagtgcc acgccgatgc cgcgcgcgac cagttgcgcg ataccctgca actcgtccag
    11581 ttcgatcacg tcttgcacat tcagccgcgc gcggcgtaaa aatcggtcca ccagcctgcc
    11641 gccgaacgat ccgcggtcgt agcggatgaa cggttcgctc ctgagcagct cgcgccatga
    11701 ccgcttgcca cgcgcaagcg ctttcggcac cagcagcacg aacggttcgg tgacgagcgt
    11761 gcgccattcg agttcagagg gcagtgcgaa cggcggtttg atgatgacgg cgagatcgag
    11821 ctcgcccgcg tccacctgtc cgagcaaacc cagcgacacg cctggcacga cgcgaatccg
    11881 ccagccgggc cgggcgtcgc gaaagcgggc cagcgcgtcg acgagaaacg acacttgcgc
    11941 ggacgcgatc gcgcccactc gcagcatgcc gctttcggca gcgcccgcgc cgcgttccga
    12001 gagccggctg tacagcgtca tcatttcttc tgcgagtgtc agcgtttcgc ggccggcgtc
    12061 gttgagcgtg gcggagcggc cagtgcgatc gaacagtgcg aagccgagct cctcttcgag
    12121 ccgctgcatc tgcgcactca cggcggattg tgtgagaccg atgcgcgctc cggcaccaga
    12181 aaacgtgccg gactggctga cggcgataaa ggtcttgagt tcgttgagca tcgtcgattg
    12241 atcgattttg gtgttggtca gctcaaatat atatcgtttt ccatcatttt tactcatggt
    12301 taaaatttgc ctcgcgcgtg caccttcctg aaccgcttcc tgagccgctt catggagcga
    12361 tacgcgagcc caatttcctt tctgtgagtt ttcccatcat gagcgttgct gaaaccgttt
    12421 tgccgccgtt ccatctggcg tttcctgtgc atagcattgc cgccgcacgc gagttctacg
    12481 gcgagctgct cggctgtccg gaagggcgga gcgcaccgga ctgggtcgac ttcaacttct
    12541 acggccatca gatcgtcgcg catctcgcgc ccgaggaaac cggccaccgg cagaccagca
    12601 aggtcgacgg cgacgcggtg ccggtgcgcc acttcggcgc ggtgctctcg atggagcaat
    12661 ggcagagcgc ggcggacaag ctgcaaaagg ccggcatcga cttcatcatc gaaccgcatg
    12721 tgcgcttcaa gggcgaggtc ggcgaacagg cgacgatgtt cttcctcgac cagtcgggca
    12781 acgcgctgga attcaaggcg tttgcgaata tgtcgtcgtt gtttgcgaag tgatgccggc
    12841 gccgggaggt tggccgaaca cggtaagtgg acgctgatta cctggctgcg actttcgcgc
    12901 aagtcctttg cacgatgcag atccgagcgc aaggtatttg cattgcgcaa ttcaatctaa
    12961 aggtatctct cgcggcatga cagcgagaca tgccttt
//
LOCUS       DATASH010000290         6525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4391841,
            whole genome shotgun sequence.
ACCESSION   DATASH010000290 DATASH010000000
VERSION     DATASH010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6525
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4391841"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VHV99_08080"
     CDS             complement(<1..284)
                     /locus_tag="VHV99_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008352980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEX3635431.1"
                     /translation="MPGQPTYSVAVRALCEFTAKRGDLDLRFTPSPTAQQGMAGHKFV
                     TVRRGERYQAELNLSDTFGPLHVRGRADGYDPGLNQLEEIKTYRGKLGAIP"
     gene            653..4822
                     /locus_tag="VHV99_08085"
     CDS             653..4822
                     /locus_tag="VHV99_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEX3635432.1"
                     /translation="MAPSAEDDGPGAAHNPSSLDFPVVGIGASAGGVQALLRFFENAP
                     SDMGMAFVVVLHLSAKHVSSADKVLQNVTRMGVLQVSGPTKIEANHVYVIAPGKSLTM
                     ADGFLNARDAEPTAGRPVSIDRFFRSLADAHGQRAMCIVMSGTGSDGSVGIANVKEKG
                     GVTLAQEPNDAEYAEMPQNAILTDQVDLVLPVVEMPQRLLDIWNNAKRIALPELEPAT
                     PGPDDRADSRGEAELALQAILATLRTRTGHDFRFYKRATMLRRIERRLQVNGVPDLIA
                     YRDLLRDNARETAALLKDMLIGVTSFFRDREAFESLERGVLPTLFHNKATGDQVRAWV
                     AGCSSGEEAYSLAMLLCEQRDVLHSSANIQVFATDIDESAVARARAGLYPESIMTDVR
                     PSTLRQFFTKTDSRYEVVKSVREKILFAMHNVLRDPPFSRLDLVSCRNLLIYLDRGVQ
                     NQVLEMFHYALYPNGYLFLGTSESADSVEDLFSVVDKKNRIYRAKAAKLPNRPSLGAP
                     SLLMQGRFMHEEEAGSALLPGRRNFSFSALHQRVLEQYAPPSVIVNRDSKIVHMSDNA
                     GRFLRYVGGEPSSNMIAVVLPELRLDLRTALFQAAQTGNSVEARRVKLQRNGHPSYVN
                     MTVRPFHDAAADADFLLVLFDEVQEAMTEDRAAAEYQTGHDTVMQQLEQELHSSKEQL
                     QTTIEQYETSMEELKASNEELQAINEELRSATEELETSKEELQSVNEELITVNAELQA
                     KIEETGNANDDLQNLITSTDIATIFVDRAMCIKRYTPSAATVFNLIGADVGRSLFDIT
                     HRLLYPELADDVGATFQSLRLTEREVATVDGRWFLARVLPYRTADDRIDGAVLSLVET
                     TARRRAEANVRAGEERLRLAAQTTNDFAIIVQDPEGHIVSWNAGAERVFGYTAAEAVG
                     QDIDVIFPADDQQAGMAAQEREIAQRDGRADDDRWLVAKGGRKVYCSGVVTPLSDPKF
                     TGYAKIVRDLTDRKTQEDARQSQLAEERAVRAQAEAANRLKDDFLAVLSHELKHPLNL
                     IHVKAEMLPRIPEARGVPAIQRTADAIQRAVLGQAKIIDDLLDLSRVRTGKLALNLAM
                     ADVAAMLKTIADAIEADAASRGVALSFEGLDEPVWTKVDPVRFDQIVWNLLSNALKFT
                     PRGGSVSVRLTSEEDELRIDVTDTGLGIEAPLLPHIFEMFSQGSEARRKTGGGMGIGL
                     ALVKQLVEMHAGSVLAQSDGAGRGTRMSVWLPLVDGLPGGKHAPQERSGSLAGVRMLV
                     VDDDSETASAFAALLELEGALVATAHDSDDALALLERNHVDLLVCDISMPGMDGYELM
                     GRVRENPRLAQLPAIALTGYTVGTGDERARASGFDLTLIKPVSLDALIAAAEQVFFWR
                     GAQGGGTHERR"
     gene            complement(4867..5997)
                     /locus_tag="VHV99_08090"
     CDS             complement(4867..5997)
                     /locus_tag="VHV99_08090"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HEX3635433.1"
                     /translation="MTLGILVLNSGSSSLKFGVYMPGEKSGATPDDSDETPWLTGSAK
                     GIGRDDGSLTMRSSDGAIDVTQNHVMESQHDALQRVADALHAHLDQPLAAVGHRVVHG
                     GPHLSDHALITPAVKQTLRDAVQFAPLHLPQALELLDLAQQIFNDVPHYACLDTAFHR
                     TMPARATHLALPKRYADEGVVRYGFHGLSYESIVATLGNDLPARLVVAHLGSGASLCA
                     IRDGRSIDTSMGLTPTGGIPMATRTGDLDPGVLVYLMRTGTLDADALEHLINHESGLK
                     GLSDSNNDMQALLQRSDADAELAVDIFCTAVRKTIGAYAALMGGLDRVVFTGGIGEHA
                     ASVRERICDGLEFLDVQFDVIETQEERQIARHCRALLRQAAV"
     gene            complement(6003..>6525)
                     /locus_tag="VHV99_08095"
     CDS             complement(6003..>6525)
                     /locus_tag="VHV99_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016354735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HEX3635434.1"
                     /translation="DSEPDVVMACAGDIATMESLAAVELLKGHFPDLRIRFVNVVDLF
                     RLMPDTDHPHGLSGRDFDSIFSREKPVIFNFHSYPSLVHKLTYNRTNHENMHVHGYRE
                     RGNINTPFELAIINQVDRFSLCIDVIDRVPRLRDTGAHAKNWLKDQINASITYAHAEG
                     IDRDEIRNWVWKG"
BASE COUNT         1266 a         1963 c         2119 g         1177 t
ORIGIN      
        1 gggatggcgc cgagcttgcc gcgatacgtc ttgatctctt ccagctggtt caggccgggg
       61 tcgtagccgt ccgcgcggcc gcgtacgtgc agtggaccga aggtgtcgga aaggtttagt
      121 tcggcttggt agcgctcgcc gcggcgaacg gtgacgaact tatgccccgc catgccttgt
      181 tgcgcagtag gcgagggcgt gaagcgcagg tcgaggtcac cgcgcttggc ggtgaattcg
      241 cacaacgcgc gtaccgcgac ggagtaggtg ggttggccgg gcatgattac tgtatggata
      301 tacagtaaat attatcacga tgcgatctgc tggaaacatc gtacggaatg ccggttgcta
      361 gcgctccagg gcggctccag agtacgaagt tgcagacatc ggctatcgat tcgggccgca
      421 ttaaaaagcg cctccacgtc cacgggaaat gccgcgtgaa tataatgagc gccgcccgtg
      481 cctctacaga cacggatgat ccgatgtcct cgcgcagcca ggcgcgcacc tcggatgcgc
      541 gctgcttcgc ccgtgcgata gaaagcacgt tcgttcaaac gcgtagtggt atctttaacc
      601 cgtcaaagca ccctgaaccg aggccgctgg agtgagcgaa actacgacat caatggcgcc
      661 gtcggcagaa gacgacgggc cgggcgcggc tcataacccg agttcgctgg atttccctgt
      721 tgtcgggatt ggcgcgtccg cagggggcgt gcaggcgctg ttgcgcttct tcgagaacgc
      781 gccgtccgac atgggcatgg cgtttgtggt ggtcctgcac ttgtcggcga agcatgtcag
      841 cagcgcggac aaggtgctgc agaacgtgac ccgcatgggc gtgctgcaag tcagcgggcc
      901 gaccaagata gaagcgaacc acgtctacgt tatcgcgccg ggcaagagcc tgacgatggc
      961 ggacggtttc ctgaacgcac gcgacgctga gcccacggcg ggccggccgg tctctatcga
     1021 ccggtttttc cggagcctgg cggacgcgca tggtcagcgc gccatgtgca tcgtgatgtc
     1081 cggcacaggc agcgacggct cggtcggcat tgccaacgtg aaggaaaagg gcggtgtgac
     1141 gctcgcgcag gagccgaacg acgccgaata cgcagagatg ccgcagaacg cgatcctgac
     1201 agatcaggtc gacctggtgc tgcccgtggt ggagatgccg caacggctgc tcgacatatg
     1261 gaacaacgcc aagcgcatcg ccctgcccga actcgaaccg gcaacgccgg gtccggacga
     1321 ccgggccgac agccggggtg aggccgaact tgcgttgcag gccattttgg cgacgttacg
     1381 cacccgcacc ggccacgatt tccgtttcta caagcgtgca accatgctgc gccggatcga
     1441 gcggcgcctg caagtcaacg gcgtgcctga cctcattgcc taccgtgacc tgctgcggga
     1501 taacgcgcgc gaaaccgcgg ccttgctcaa ggacatgctg attggcgtga ccagtttttt
     1561 ccgcgataga gaagcgttcg agtcgctcga gcgcggcgtg ctgccaacct tgttccacaa
     1621 caaggccacg ggcgatcagg ttcgcgcgtg ggtcgcggga tgctcgtcgg gcgaggaggc
     1681 gtattcgctt gccatgctcc tgtgcgagca gcgcgacgtg ttgcattcct cggccaacat
     1741 ccaggtgttc gccaccgata tcgacgaaag cgccgtagcg cgcgcacggg ccggactgta
     1801 ccccgagtcc atcatgaccg acgtacgccc ctctacgctg cggcaatttt tcaccaagac
     1861 cgattcgcgc tacgaggtgg tcaaaagcgt gcgcgagaag atcctgttcg ccatgcacaa
     1921 cgtgctgcgc gatccgccgt tctctcgcct cgacctggtt tcttgccgca acctcctgat
     1981 ctatctcgac cgtggcgtgc agaatcaggt gctggaaatg ttccactacg cgttgtaccc
     2041 caacgggtat cttttcctgg gcacttcgga gtcggcagac tcggtcgaag acttgttcag
     2101 tgtggtcgac aagaagaatc gtatataccg ggcgaaggcg gcgaagctgc cgaaccgccc
     2161 gtcgttgggc gcaccgtcgc tgctgatgca ggggcgcttc atgcatgagg aagaggcggg
     2221 ctccgctctg ctcccggggc gccgcaattt ttcgttcagt gcgttacacc agcgcgtgct
     2281 cgagcaatac gcgccgccca gcgtgatcgt gaatcgcgat tcgaagattg tgcatatgtc
     2341 ggacaacgcg gggcgtttcc tgcggtatgt cggaggcgaa ccttcgagca acatgattgc
     2401 cgtcgtgctg cccgaactgc ggctcgatct gcgtaccgcg ctgttccagg ccgcgcagac
     2461 gggcaacagc gtggaggccc ggcgcgtcaa gctgcagcgc aacggacatc cttcatacgt
     2521 caacatgacc gtgcgcccgt tccatgacgc cgccgccgac gccgatttcc tcctcgtcct
     2581 gttcgacgag gtccaggaag cgatgacgga agaccgggct gcagcggagt atcaaacggg
     2641 ccatgacacc gtgatgcagc agctcgaaca agagctgcac tcgagcaagg agcaactgca
     2701 gaccacgatc gagcagtacg agacgtcgat ggaagagctg aaggcatcca atgaagaact
     2761 gcaggcgatc aacgaggagt tgaggtcggc aacagaagaa ctcgaaacca gcaaggaaga
     2821 gttgcagtcg gtgaacgagg aactcatcac ggtcaacgcg gaactgcagg cgaagatcga
     2881 ggagacgggc aatgccaatg acgacttgca gaacctgatc acctccactg acatcgccac
     2941 cattttcgtc gatcgcgcga tgtgcatcaa acgctacacg ccgagcgcgg ccaccgtgtt
     3001 caacctgatc ggcgcggatg tcgggcgctc gttgttcgac atcacgcacc ggctgcttta
     3061 tcccgagctg gccgacgatg tgggcgcgac gttccagtcg ttgcgcctga ccgagcgcga
     3121 ggtcgccacg gtcgacggcc gctggttcct cgcgcgtgtg ctgccttacc ggacggcaga
     3181 cgaccggatt gatggcgcag tgctcagtct cgttgaaacc acggcgcgcc gccgcgcgga
     3241 ggcgaacgtg cgggcgggcg aggagcggct gagactcgcg gcgcaaacga cgaacgactt
     3301 cgcgatcatc gtgcaggacc ctgagggcca cattgtcagc tggaatgccg gcgccgagcg
     3361 tgtgttcggt tatacggcag cggaagcggt cggccaggac atcgacgtga tctttcccgc
     3421 ggatgatcag caggcgggca tggcggcgca ggaacgcgag atcgcgcaac gcgatggccg
     3481 tgccgacgat gatcgctggc ttgtcgcgaa gggcggccgc aaggtgtatt gcagcggcgt
     3541 ggtgacgccg ttgtccgatc cgaagttcac gggttacgca aagatcgtga gagatctcac
     3601 ggaccgcaag acgcaggaag acgcgcggca gtcgcaactg gcggaggaac gggcggtgcg
     3661 ggcacaggcg gaagcggcga accggctcaa ggacgatttc ctggccgtgc tgtcgcacga
     3721 attgaagcac ccgctcaacc tgattcacgt gaaggcggaa atgttgccgc gcatccccga
     3781 ggcgcggggt gtgccggcga tccagcgcac ggccgacgca atccagcgcg cggtgctcgg
     3841 tcaggcgaag atcattgacg acttgctgga cctgtcgcgc gtgcgtaccg gcaaactggc
     3901 gttgaacctg gcgatggccg acgttgcagc catgctgaaa accatcgccg atgcaatcga
     3961 ggcggacgct gcatcaaggg gcgttgcgtt gtcgttcgag ggtctggatg aaccggtgtg
     4021 gaccaaggtg gaccccgtac gtttcgacca gatcgtgtgg aacctgctga gcaacgcgct
     4081 gaaatttacg ccgcgcggcg gctcggtttc cgttcgcctc acgagtgaag aggacgagtt
     4141 gcgtatcgat gtcacggata ccgggctcgg aatcgaagcg ccgctgttgc cgcatatctt
     4201 cgagatgttc tcccagggca gcgaagcccg tcgcaagacc ggcggcggca tgggcatcgg
     4261 cctcgcgctg gtcaagcaac tcgtggaaat gcatgcggga agcgtgctcg cgcagtcgga
     4321 cggggcagga cgcggtacgc ggatgagcgt ctggctgccg ctggtcgacg gccttcccgg
     4381 cggaaagcat gcgccgcagg agcgcagcgg atcgctggca ggcgtgcgta tgctggtcgt
     4441 cgacgatgac tccgaaaccg ccagcgcatt cgcggcgttg ctggaactgg aaggcgcgct
     4501 ggtcgccacc gcccacgaca gcgacgacgc gctcgcgctg ctcgaacgca accacgtgga
     4561 tctgctggtc tgcgacatca gcatgcccgg catggacggc tacgaactca tgggccgggt
     4621 ccgggagaat ccccgcttgg cccagttgcc cgctattgcg ttgacgggct acaccgtggg
     4681 taccggcgac gagcgcgcac gcgcgtcggg cttcgatctg acgctgatca agccggtctc
     4741 ccttgacgcg ctgatcgccg ccgccgagca ggtgttcttc tggcgcggcg cacagggcgg
     4801 tggcacgcac gagcgtcgct gatggttgat gagtccacca acaacccagc ctctgacgag
     4861 ccgctttcag acggccgctt gcctgagcag cgcgcggcaa tgacgcgcaa tctgccgctc
     4921 ttcctgcgtc tcgatcacgt cgaactgaac atcgagaaat tcgagtccgt cgcaaatgcg
     4981 ttcacgcacg ctcgcggcgt gctcgccaat accgccggtg aacaccacgc ggtcgagacc
     5041 gcccatcaac gcggcatacg cgccgatggt cttgcgcaca gcagtacaga agatatcgac
     5101 ggcaagttct gcatctgcat cgctgcgttg caaaagcgcc tgcatgtcat tgttcgagtc
     5161 ggacagaccc ttgaggccgc tttcgtgatt gatcaggtgt tcgagcgcat cggcgtcaag
     5221 cgttccggtc cgcatcagat aaaccagcac accaggatcg agatcgccgg tgcgtgtggc
     5281 catgggaatg ccgcccgtcg gcgtgagacc catgcttgta tcgattgaac ggccatcgcg
     5341 gatcgcgcac aggcttgcac cgctgccgag atgcgcgacc accaggcgcg ccggaagatc
     5401 gttgccgagc gttgcgacga tcgactcgta cgagaggccg tggaaaccgt atcggaccac
     5461 cccttcgtca gcgtagcgtt tcggcagcgc gaggtgagtc gcgcgtgcgg gcatggtccg
     5521 gtggaacgcg gtgtcgaggc aggcgtaatg cggcacgtcg ttgaaaatct gctgcgccag
     5581 atcgagcagc tccagcgcct gcggcaaatg caacggcgca aattgcaccg cgtcgcgcaa
     5641 ggtctgtttg accgcgggcg tgatcagcgc atggtcgctc aggtgcggac cgccatgcac
     5701 tacccgatgc cccaccgctg ccaacggctg gtcaagatgc gcatgcagcg cgtcggccac
     5761 gcgctgcaac gcgtcgtgct gggattccat cacgtggttt tgcgtgacat cgatcgcacc
     5821 atccgatgaa cgcatggtca gcgagccgtc gtcgcggccg atgcccttcg cgctgccggt
     5881 cagccacggc gtttcatcgc tgtcgtctgg cgtggcgccc gacttctcgc cgggcatata
     5941 cacgccgaat ttaagcgacg acgaaccgct gttcagcacc aggattccca gggtcatggt
     6001 ctttagcctt tccaaaccca gttacgtatt tcatcgcggt caatgccttc cgcgtgggcg
     6061 taggtaatgc tcgcgttgat ctggtccttg agccagttct tcgcgtgggc accggtatcg
     6121 cgaagccgcg gcacccgatc gatcacatcg atacacagcg aaaagcggtc gacctggttg
     6181 atgatcgcca gctcgaacgg cgtattgatg ttgccgcgct cgcgataacc atgcacatgc
     6241 atgttctcgt gattcgtgcg gttatacgtg agcttgtgaa cgagcgacgg atacgagtgg
     6301 aagttgaaga tgaccggctt ctcgcgcgag aaaatagaat cgaaatcgcg accggacaaa
     6361 ccgtgcggat gatcggtatc gggcatcaac cggaacaggt ccaccacgtt cacgaagcgg
     6421 atcctgagat ccgggaaatg tcctttgagc agttcgacgg cggcaagcga ttccatggtc
     6481 gcgatatcgc cggcgcaggc catcaccacg tccggctcgc tgtcc
//
LOCUS       DATASH010000291         5792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_631977,
            whole genome shotgun sequence.
ACCESSION   DATASH010000291 DATASH010000000
VERSION     DATASH010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5792
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631977"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1157)
                     /locus_tag="VHV99_08100"
     CDS             complement(<1..1157)
                     /locus_tag="VHV99_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635435.1"
                     /translation="MSVLKQVSPSTALPDAAFALRPSACPGLLRIVAARDGGICRIKL
                     PGGELSAAQAAVIADVSEHHAAGVVELTNRANLQVRGVRGGHEAALIAALVDAGLGPM
                     PASMSMSMSMPASGAADAAVGLRVGEAAASTARSADLRHASSNLQLASAADDVRNVMI
                     SPAAGRDPFALFDTRPLCAELLALLQNDARFAALSPKFALLLDGGERLARLDHPHDVW
                     LAATQARDGVRFVFGLAGCPPVMANADVDADAGSDADARARANTDADADADADADADA
                     DAFQYAEHADSRDSAGRPRTKSTGALAAVPPSQVAALVRALLHTFLDLAAADATRMRH
                     VLSAHSIDAVLQHAQTYVDFPLSRDTSLANWQRGTPADATLRLGAHAQRVVG"
     regulatory      complement(1240..1419)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1504..2727
                     /gene="cbiE"
                     /locus_tag="VHV99_08105"
     CDS             1504..2727
                     /gene="cbiE"
                     /locus_tag="VHV99_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013341214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6y C5,15-methyltransferase
                     (decarboxylating) subunit CbiE"
                     /protein_id="HEX3635436.1"
                     /translation="MPAWLTVVGIGDDGFAGLGRAARRALLEASVVYGGERHLAMLPA
                     RLAARRAAWPRPFDLAPLLAERSGSVCVLASGDPMIFGVGATLARQLPAGELRVLPAP
                     SSLSLAAARLGWPLQDVITVSLVGRPLPTLNAHLHDGARVFVLSTDGRTPAALAELLD
                     ARGFGATRMTVLEHLGGALERCIDGRADQWSPGEAAALNLIALECRATEGAPRLPLTS
                     GLPDDAFRHDGQLTKRDVRAITLARLAPTPGELLWDVGAGSGSIGIEWMRAHRTCRAI
                     AIEGHAERQRFIEHNRDALGVPGLQLVAGRAPDALEGLAVPDAVFIGGGVTAPGVLET
                     CWARLRDGGRLVANAVTLQGEAALVAWREQHGGTLTRIALADAQPLGGFDTWRQALPI
                     TLLDVMKPADSRPQA"
     gene            2727..3815
                     /locus_tag="VHV99_08110"
     CDS             2727..3815
                     /locus_tag="VHV99_08110"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HEX3635437.1"
                     /translation="MREETPESPAPLRSGYTTGSCATATSLAAARLLLAGIVSEIAEI
                     VLPKGQHVPMRLVFCRLTADGAEAGTVKDAGDDPDVTHGAIVFARVRLRAEPGVIFRA
                     GPGVGTVTRAGLTLPVGEPAINPVPRRMMTEHLIALAADHGYEGGFEVTIGVEGGEAL
                     ALKTMNPRLGILGGLSILGTTGIVRPFSCSAYIASIHQGIDVARANGYLHLAACTGNA
                     SEDAMRAHYGLPDIALIEMGDFVGAVLKHMKRAPVERLSVCGGFGKLSKLAAGHLDLH
                     SRNSSIDLERLAAWAAGQGADAALQAAILAANTSQQAVALAHAQHVPLGDIVCRHALA
                     VARDIVPPQVAVEMFAIDRQGNLIGVAQ"
     gene            3812..4540
                     /locus_tag="VHV99_08115"
     CDS             3812..4540
                     /locus_tag="VHV99_08115"
                     /EC_number="1.3.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014971809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016994 - precorrin-6A reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-6A reductase"
                     /protein_id="HEX3635438.1"
                     /translation="MMRILLLGGTGDALRLARQLGPGHVYSLAGLGKVPDDLSCDVRV
                     GGFGGGEGMARYLEDERIGLVIDATHPYAARISANAANASGAARVPCWALRRAGWLPQ
                     AGDDWRMVDAWAELTGALAPFKRPLFTLGREPLAHLDEIPPQQFWTIRCLEAHETHPR
                     ARILATRGPFTLEGERALFGAEAFDVVVSKNSGGSATEAKLEVARERGLPVVMRRRPE
                     LPAVDREFANVADLLEALQVQGLG"
     gene            4586..5314
                     /gene="cobM"
                     /locus_tag="VHV99_08120"
     CDS             4586..5314
                     /gene="cobM"
                     /locus_tag="VHV99_08120"
                     /EC_number="2.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011545413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046026 - precorrin-4
                     C11-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-4 C(11)-methyltransferase"
                     /protein_id="HEX3635439.1"
                     /translation="MTVFFIGAGPGDPELITVKGQRLVRGCPVILYAGSLVPAAVLEG
                     HGAEQVVNTAELDLDQIVALLKAAHDKGQDVARVHSGDPSLYGAIGEQIRRLRELGIP
                     YEIVPGVTATAACAAALGCELTLPDISQTLILTRFASKTTMPAGEQLADLARHRATMA
                     IHLGVRHLARIVDELRPHYGGACPIAVIYRASWPDEEKITGTLDDIVGKVQSTHIERT
                     ALILVGQVLAAEGFAESTLYAKGG"
     gene            5428..>5792
                     /locus_tag="VHV99_08125"
     CDS             5428..>5792
                     /locus_tag="VHV99_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018425210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEX3635440.1"
                     /translation="MSRLMCAALTCAVGLLWAGPAVPADHAPWHVGEGVRVFHPAVAR
                     HWRGAQTRALVTSVWYPADPALPEAAHNLGAPGHPLFRGHPAVVGAPLSSARATYPLL
                     VLSHGTGGSADSLDWLASAL"
BASE COUNT          928 a         1859 c         2030 g          975 t
ORIGIN      
        1 cccacgactc gctgcgcgtg cgcgccgagt cgcagcgtgg catcggcagg cgtgccgcgt
       61 tgccagttcg cgagtgacgt gtcgcgtgac agcgggaaat cgacgtacgt ttgagcgtgc
      121 tgcaatacgg cgtcgatgga atgtgcggaa agcacatgac gcatgcgcgt ggcatcggcg
      181 gcggccagat cgaggaacgt atgcagcagt gcgcggacaa gggcggcgac ttgcgatggc
      241 ggcacggctg cgagggcgcc ggtgcttttc gtgcgaggac gcccggcgga atcacgcgaa
      301 tcggcgtgtt cggcatactg gaacgcatcg gcatcggcat cggcatcggc atcggcatcg
      361 gcatcggcat cggtattggc acgcgcacgg gcatcggcat cagaacccgc atcagcgtca
      421 acatcagcat tcgccatcac aggcggacaa cccgccaaac caaacacaaa tcgcacaccg
      481 tctctggctt gcgttgccgc cagccagaca tcatgcggat gatcgagcct cgcaaggcgc
      541 tcaccaccgt cgagcaacaa cgcaaatttc ggcgacagcg ccgcgaaacg cgcatcgttt
      601 tgcagcagcg cgagaagctc ggcgcagagc ggtctcgtgt cgaacagcgc gaacggatca
      661 cgcccggctg cggggctgat catgacgttg cgcacgtcgt cggcggcgga ggctagttgc
      721 agattcgaag atgcatgccg caagtccgcc gaccttgccg ttgaagcggc cgcttcgccc
      781 actctcaatc cgacagctgc gtccgctgcg cccgacgccg gcatcgacat cgacatcgac
      841 atcgacgccg gcatcggccc gagccccgca tcgaccagcg ccgcgatcag cgccgcctca
      901 tgcccgccgc gcacgccacg cacctgcaga ttggcgcgat tggtcagctc gaccacgcca
      961 gccgcatggt gctcgctaac gtctgcgatc accgccgcct gcgccgcgct cagctcgccg
     1021 ccgggcaatt tgatccggca aatcccgcca tcgcgggcag cgacgatgcg cagcagcccc
     1081 gggcacgccg agggccgcag cgcgaaggcc gcgtcgggca aagcagtgga gggcgaaact
     1141 tgcttcaaga cggacaccgg gtttagcgtc gcgcgatggc ccgagcgccg cccgggcggg
     1201 cattgctgcg gcatcggtac accccgcccg atggtggctg gcacgaacag tctggccggc
     1261 aggtctcctg gctggcaggt cgtggtccgc cgctgccttc ccggtcgaac cagtggcgcg
     1321 gagcgcaggc ggactcgctg catacagttg cgggggcagc cacagtggca ctgtgttccc
     1381 tcttcggccc cgaagggcac cggcggctgt attatgcctt ttttcgaaca gcacaacgag
     1441 gctggacgct ttgatcggcg tggcacagcg cactatttga agatacagaa caaggatgga
     1501 tgcatgccgg cgtggctgac cgtggtgggc ataggcgatg acggcttcgc cggtttgggg
     1561 agggccgcgc ggcgtgcttt gctggaggcg tcggtggtct acggcggcga acggcatctg
     1621 gcgatgctgc ccgcgcgtct cgccgcgcgc cgtgcggcat ggccgcggcc gttcgatctg
     1681 gcgcctttgc tggccgagcg tagcggctcc gtgtgcgtgc tggcaagtgg tgacccaatg
     1741 atattcggcg tcggcgcgac cctggcgcgg cagttgcccg cgggcgagtt gcgggtgctg
     1801 cccgcaccgt cgtcgctgtc gctggcggcc gcgcggctgg gctggccgtt gcaggatgtc
     1861 atcacggttt cgctggtggg aagaccgttg ccgacgctga atgctcatct gcacgacggc
     1921 gcgcgtgtct tcgtgctgag caccgacggg cgcacgcccg cggcgctggc cgaactgctg
     1981 gatgcgcgcg gtttcggcgc gacgcggatg accgtgctgg aacatctggg cggcgctctg
     2041 gagcggtgta tcgatggccg cgcggatcag tggtccccag gcgaagcagc ggcgctgaac
     2101 ctgatcgcac tcgaatgccg ggccacggaa ggcgcgccgc gtttgccgct gacctccggc
     2161 ttgcccgatg acgcctttcg ccatgatggt caattgacca aacgcgacgt gcgcgcgatc
     2221 acgctggcgc gtctcgcgcc gacgccgggc gaattgctat gggatgtggg cgcgggcagc
     2281 ggctcgatcg gcatcgaatg gatgcgcgcg caccggactt gccgtgcgat cgcaatcgaa
     2341 gggcatgcgg agcggcagcg gtttatcgaa cacaatcgcg atgcgttggg cgtgccgggc
     2401 ttgcagctgg tggccggccg cgcgccggat gcgctggaag gattggcggt gcccgatgcc
     2461 gtgttcatcg gcggcggtgt gacggcgccg ggtgtgctgg agacgtgctg ggcgcgtctg
     2521 cgtgacggcg ggcggctggt cgcgaatgcg gtcactttgc agggcgaggc ggcgctcgtg
     2581 gcgtggcggg agcagcatgg cggcacgctg acccgcatcg cgctcgccga cgcacagccg
     2641 ctcggcggct tcgatacgtg gcgccaggcg ctgccgatca cgttgctgga tgtcatgaag
     2701 ccggcggact cccggcccca agcgtaatgc gcgaagaaac gcccgaatca cccgcgccgc
     2761 tgcgtagcgg ctacacaacc ggcagttgcg ccaccgcgac gtcgctcgcg gccgcgcgcc
     2821 tgttgctcgc gggtatcgtc agcgagatcg cggagatcgt gttgccgaag ggccagcatg
     2881 tgccgatgcg gctggtgttt tgccgcctca ctgcggacgg cgcggaagcc ggcacggtga
     2941 aagacgccgg cgatgacccc gacgtgaccc acggcgcgat cgtcttcgcc cgcgtgcgcc
     3001 tgcgcgccga gccgggtgtg atattccgcg cggggccggg cgtcggcacg gtcacgcgcg
     3061 cgggcctcac gctgccggtc ggcgaaccgg cgatcaatcc tgtaccgcgc aggatgatga
     3121 ccgagcatct gatcgcgctc gcggccgacc atggctacga aggaggtttc gaagtgacga
     3181 tcggcgtcga aggcggcgag gcactcgccc tgaagacgat gaacccgcgc ctcggcatcc
     3241 tcggcggtct gtcgattctc ggcaccacgg gcatcgtgcg gccgttctcg tgttcggcct
     3301 atatcgcgtc gattcatcag ggcatcgacg tcgcgcgtgc caacggttat ctgcatctcg
     3361 cggcctgcac cggcaacgcg agcgaagacg cgatgcgcgc gcattatggt ctgccggata
     3421 tcgcgctcat cgaaatgggc gacttcgtcg gcgcggtgct caagcatatg aagcgcgcgc
     3481 cggtcgagcg cttgagcgtg tgcggcggct tcggcaagct cagcaagctg gcagccgggc
     3541 acctcgatct gcatagccgc aattcgagca tcgatctgga gcggcttgca gcatgggccg
     3601 ccgggcaggg cgccgacgct gcattgcagg cggcgatcct cgcggccaat acgagccagc
     3661 aggccgttgc gctggcgcac gcgcaacacg tgccgctcgg cgatatcgtc tgccggcatg
     3721 cgcttgctgt cgcgcgcgat atcgtgccgc cgcaggttgc ggtcgaaatg ttcgcgatcg
     3781 accgccaggg caatctgatc ggagtcgcgc agtgatgcgg atcctgctgc tcggtggcac
     3841 cggcgacgcg ttgcgtctcg caagacagct cggccccggg cacgtgtaca gtctcgcggg
     3901 cctcggcaag gtgccggacg atctctcctg cgacgtacgc gtgggcggct ttggcggggg
     3961 cgaaggcatg gcgcgctatc tcgaagacga acgcatcggc ctcgtgatcg acgcaacgca
     4021 tccctacgcc gcgcggatta gcgccaatgc ggcgaacgcg agcggcgccg cgcgcgtgcc
     4081 atgctgggca ctgcgtcgcg cgggatggct gccgcaagct ggcgacgact ggcgcatggt
     4141 cgacgcctgg gccgaactca cgggcgcgct cgctccgttc aaacggccgc tgttcacgct
     4201 cggccgcgaa ccgctcgcgc atctcgacga aatcccgccg cagcagttct ggacgatacg
     4261 ctgccttgaa gcacacgaga cccatcctcg tgcgcggatt ctggcgacac gcggcccatt
     4321 cacgctcgaa ggcgagcgag cgctgttcgg cgccgaagcg ttcgatgtcg tcgtcagcaa
     4381 aaacagcggc ggcagcgcca cggaagcgaa actcgaggtc gcgcgcgaac gcggtttgcc
     4441 ggtcgtcatg cggcggcggc ctgaattgcc cgcagtcgat cgcgagttcg caaacgtggc
     4501 cgacttgctg gaagcattgc aagtgcaggg cttaggttaa atggcgcatg aatgacatgc
     4561 cggccgaacg aatctggagt attgaatgac ggtgtttttt atcggcgcgg gtcccggcga
     4621 cccggaactg atcacagtga aaggccagcg cctcgtgcgt ggctgcccgg tgatcctgta
     4681 cgccggctcg ctggtgccgg ccgctgtgct ggaaggacat ggcgcggagc aggtcgtgaa
     4741 taccgccgag ctcgacctcg atcagatcgt cgcgttactc aaggcggcac acgacaaagg
     4801 gcaggacgtg gcgcgcgtgc attccggcga cccgtcgttg tatggcgcga tcggcgaaca
     4861 gatccgcaga cttcgcgaac tcggcattcc ctacgagatc gtgcccggcg tgacggcgac
     4921 cgcggcgtgc gcggcggcgc tcggttgcga gctcacgttg cccgatattt cgcagacgct
     4981 gatcctcacg cgcttcgcaa gcaaaacgac catgcccgca ggcgaacaac tcgccgacct
     5041 cgcgagacat cgtgcgacga tggccattca cctcggcgtg cgccatctcg cgcgcatcgt
     5101 cgatgaattg cggccgcatt acggcggtgc gtgtccgatc gcggtgatct atcgcgcgag
     5161 ctggccggat gaggagaaaa tcacagggac gctcgacgat atcgtcggca aggtgcagtc
     5221 gacccacatc gagcggaccg cgctgattct ggtgggacag gtgctcgccg ccgaaggatt
     5281 cgcggagtcg acgttgtatg cgaaaggcgg ctgagccgat tcgggtgggc cgcgatttca
     5341 agtaggcggg atggacaacc cgctggttgc cggttcgcac catgcctgtg tcacggatct
     5401 catgatcccc atccgcaagg actgactatg tctcgtctta tgtgtgctgc cctcacgtgt
     5461 gcggtggggt tgttgtgggc gggccccgcc gtgcccgcgg atcatgcgcc ctggcatgtt
     5521 ggagagggcg tgcgtgtttt tcatcctgcc gtggcccgtc attggcgcgg cgctcaaacc
     5581 cgggcgctgg tgacgagcgt ctggtatccg gccgatccgg cattgccgga ggccgcgcat
     5641 aacctcggcg cgccaggtca tcccttgttc cgcgggcatc cggccgtcgt gggtgcgccg
     5701 ctgtccagcg cacgcgcaac ctatccgctg ctcgtgctgt cgcacggtac cggcggcagc
     5761 gcggacagcc tcgactggct ggcctcggcg ct
//
LOCUS       DATASH010000292        20782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4786017,
            whole genome shotgun sequence.
ACCESSION   DATASH010000292 DATASH010000000
VERSION     DATASH010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 20782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20782
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4786017"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..514)
                     /locus_tag="VHV99_08130"
     CDS             complement(197..514)
                     /locus_tag="VHV99_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017330821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635441.1"
                     /translation="MTDATPQSTFRGLPLTPEQDAEVRHYIKKKKQHGAPWDTPELEA
                     MLRDMLQPPSDDDGGLGSTVDETKAATERATASIDETMEPIEACEERNAAMETEGMRG
                     PKR"
     gene            942..1202
                     /locus_tag="VHV99_08135"
     CDS             942..1202
                     /locus_tag="VHV99_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009907034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX3635442.1"
                     /translation="MRLIERDERQAFLATLQRKGLAEGEFELLETDTTDPKGDENVGL
                     QGYVTVTRLSTQIAREYPIGFETGWVQHFRQDLEAGVFDKPE"
     gene            1254..1586
                     /locus_tag="VHV99_08140"
     CDS             1254..1586
                     /locus_tag="VHV99_08140"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3635443.1"
                     /translation="MEAGSNGIEATENSGRRPKIRITASLRDDQSVLLEVIDNGSGIV
                     GDDSIFDAFVTTKSKGMGIGLAVSRSIVEAHEGHLTAANNDGHGATFSVVLPVSPLPI
                     SQQGVGIQ"
     gene            complement(2275..2913)
                     /locus_tag="VHV99_08145"
     CDS             complement(2275..2913)
                     /locus_tag="VHV99_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635444.1"
                     /translation="MTLELHMTRSRGRFLFGAGAALVTLGCALHSSYGYAGAFDDLKQ
                     SLAKSLNDAAKKNVDAALGNTPPGSPAQVQSSGPAGTGPAQPQAADAIQPVRGNAPGA
                     SSQATGEAPAGCAKRRLSSPMKAFIVRNDAMIMTIVNDCDSELTVMTHEGRVNNGMCI
                     ASWVRAHGVKSTMPVARLCNTRMPLPKTSPYAVPCACPDGTNIDLNTMPLAD"
     gene            complement(3174..4199)
                     /locus_tag="VHV99_08150"
     CDS             complement(3174..4199)
                     /locus_tag="VHV99_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017329290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEX3635445.1"
                     /translation="MVNVLPDEARLAGEIDRLKAEFPKTRELYREVCALLFFRFGITP
                     TANRLYQLVKRGSMSTPTQVLGEFWVELREKSRVRIEHPDLPADLQAAAGELIATLWT
                     KSTTSAQAALEELRADAEAEKAAARNVVETLRAELARTETALEQRTGALLTAQVRIQE
                     LEQAQAAGEATRQALETEVARQQDEIGARDRALVQARADFSGELEKLRGSAELAEERL
                     RAAEKRALLEIERERSTGVRLHKDLAAAARRAEQGETRHREEIRALQVQLGDARHRAG
                     VVEGSLEALRHSNTAYVTELNELRLQLATVKTNQAAPPKRPRSSDETPKPRRPTSKAA
                     TRKPSTS"
     gene            complement(4590..5885)
                     /locus_tag="VHV99_08155"
     CDS             complement(4590..5885)
                     /locus_tag="VHV99_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3635446.1"
                     /translation="MTNELQARVVRKLTWRILPFIMLLYFVSFLDRVNVGFAALTMNK
                     DLGLSPTMFGLGGGIFFLGYFLFEVPSNLILNKVGARIWIARVMVTWGLVSAASAFVV
                     GPNSFYALRFLLGLAEAGFFPGIILYLSQWFPTRQRAVAAAVFMAAAPLSTAIGSPIS
                     GAIMQMPTLFGLKDWQWLFLIEAIPAVVLGFVVLKALTDSPEKARWLAADEKAWLIAT
                     LREERAGRESQAGHATGALAALRDPRVWIMSMIYFGTSAGLYTLGLWAPLIIRQFGFS
                     ALGTGALNAIPSVLAVVGMVMWARHSDRTGERTWHVVIPCVVAGVGLAWAGFADTVVA
                     VVLALILVNVGISAAKAPLWAMPSTFLSGAGAAAGIAMINSLGNLGGFVGPFAIGWLK
                     SVTGGYAAGLYVVAASLAVSAMLALAVGRRGYRTAPAPQ"
     gene            complement(5999..7093)
                     /locus_tag="VHV99_08160"
     CDS             complement(5999..7093)
                     /locus_tag="VHV99_08160"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HEX3635447.1"
                     /translation="MKTYHIATIPGDGIGKEVVPAGQRVLEAIAARSDAFRFEFENFD
                     WGGDFYRQHGVMMPADGLDAIRNKDAILFGSAGDPHIPDHVTLWGLRLKICQGFDQYA
                     NVRPTRILPGIDGPLKRCGPKDLDWVIVRENSEGEYSGVGGRAHQGHPIEVATDVSMM
                     TRAGVERILRFAFRLAQSRPRKLLTVITKSNAQRHAMVMWDEIALEVSKEFPDVKWDK
                     ELVDAATARMVNRPATLDTIVATNLHADILSDLAAALAGSLGIAPTANLDPERRYPSM
                     FEPIHGSAFDIMGKGLANPVGTFWSVVMMLEHFGETAAAQQLMNAIERITADPALHTG
                     DLGGTATTVQVTDAVCAILAAEPAAQFKAA"
     gene            7322..8233
                     /locus_tag="VHV99_08165"
     CDS             7322..8233
                     /locus_tag="VHV99_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3635448.1"
                     /translation="MTSSIQPDDLAFFSTLATSGSLTAAARELGLTTAAVSKHLSQME
                     SRAGVSLVNRTTRRMSLTPEGELYLTRARRILSEMDDLAQLLGGSQAAPKGLLRVNAT
                     LGFGRSHVAPLISRFVQRFPEVDVQLQLSVDPPPISDDVFDVCVRFGEPPDARVIARR
                     VASNRRLLCASPAYFAERRAPRSPAELSQHNCIGIRQGDDGYGLWRLTSGRGSTEKTE
                     TVRVRGNLTTNDGEIAVKWALDGHGILMRAEWDIVEYLADGRLIQVLPEYRTPGADIY
                     AVYPQQLQMTARVKAFVDMLIEALGAR"
     gene            9462..9773
                     /locus_tag="VHV99_08170"
     CDS             9462..9773
                     /locus_tag="VHV99_08170"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HEX3635449.1"
                     /translation="MRDWPYADVDGDPAGAEAVRALARHRIEEACDRLDTNLADDRDC
                     LVGGTLSTVDFLAVVLMRWTRNMPRPALGWPHLARYIRGLRALPSFIELNTREGLTRM
                     A"
     gene            complement(9852..10664)
                     /locus_tag="VHV99_08175"
     CDS             complement(9852..10664)
                     /locus_tag="VHV99_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytochelatin synthase family protein"
                     /protein_id="HEX3635450.1"
                     /translation="MSPSGWLTAFLTATLAACAQLPPAANQVAEQTAHQDSYQVPAAS
                     AASAARPADGPLPVPPNLIALTQPAGQKRLIATPHNQSYWPLSEYFETQRNEAYCSVA
                     TSVMALNALGIRRPESTQYPDFPYFSQEDFFRNVAPQVANAARVSREGMTLDQLSAAL
                     SAFPVEVKKFHAADLTLAQFRDLIRDTTGHSDRFALLNFRRVEIGEAGGGHWSPLAAY
                     DAASDSALLLDVARYKYPAVWVPLTQLYAAAQAVDNVSGLSRGIVVVGTRAN"
     gene            complement(10678..10899)
                     /locus_tag="VHV99_08180"
     CDS             complement(10678..10899)
                     /locus_tag="VHV99_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635451.1"
                     /translation="MSDFDKLFGRARQDRPPEYSKRPNKRTAGTQTHASIGFVLKIRG
                     VADAQEALEDGNRSKLIHCGKGNVQIFLD"
     gene            10928..11224
                     /locus_tag="VHV99_08185"
     CDS             10928..11224
                     /locus_tag="VHV99_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015877176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HEX3635452.1"
                     /translation="MSDLKSAASGAGRDPASLDTSDKKQCQAHGPDAPIAAVPSPCID
                     VCKIDGNTGMCIGCLRTRDEIRGWKNMTDDLRLEMIEELSHRKANLEASKARML"
     gene            complement(11249..12394)
                     /locus_tag="VHV99_08190"
     CDS             complement(11249..12394)
                     /locus_tag="VHV99_08190"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX3635453.1"
                     /translation="MAPEAGKTSIPIPERARTSRAARGRALAIRFRPGPAAIAAAALV
                     CLLIGLLYLALQIRTIFSDQLKQEYAELVLDAVERAESARSVVGIWQQLPDAMATRAA
                     GYRHARTELARRLTSLAALVNASPSAAPHVSSSALAPDADLRDTDAWLTAVSNGWRAQ
                     RDAASADARARITYVAHTLIASAALLFCMLITALGMYAKRNRLLAGQSREFEQAALHD
                     AMTGLPNRRKLFATLEAAATRSLAGGSPRKIAVLYVDLDGFKQVNDSLGHRVGDEFLI
                     AVSKRFRESVRKVDVVARIGGDEFAVLIREFSTNAELGEIARRLIDCVVQTDEEMGNG
                     LVRASIGIASFPDPVGDYHSLVTAADEAMYRVKRSGKNSYAFAERVS"
     gene            complement(12354..13052)
                     /locus_tag="VHV99_08195"
     CDS             complement(12354..13052)
                     /locus_tag="VHV99_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635454.1"
                     /translation="MFNDTDLPKFAVEHPDVADDAGAEPPHAAPPVRFGRLALCVAAA
                     SALTFGVVGTVAYGVWFNHDQQAYADAMAGARQALGTAALATAGPPASAKVIAPAKVA
                     TAVAASRPAATAAAPDEAGKQQASWAGPVTQLAKPAARQISVADATPAMAPSSAPAAH
                     RAAPPSNTVAQPQLASSRAARDARLAQQDRRSSSTADARRKGSLFARMGSFFRRVSYR
                     QHGTGSRQDIYSHP"
     gene            13597..14586
                     /locus_tag="VHV99_08200"
     CDS             13597..14586
                     /locus_tag="VHV99_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013094346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEX3635455.1"
                     /translation="MSRTIMFEKAGGPEVLEFVETPVAAPGPHEVRIKVEAIGINRAE
                     SMWRNDAYIEPVRFPAGLGYEAAGIVDAVGAEVQGVAPGDKVNVMPSFSMNEYFTYGE
                     VILVPDYAVVKHPESLSFAEAASVWMMFVTAYGALIEDAKVTSGDFVIVPAASSSVGL
                     AAIQIANYAGATSIALTRMSGKRQQLLDAGAAHVIATEETDLVAEVMRITDGKGARVA
                     FDPVGGPTFAKLIAALSFQGTAYIYGALSDEVTPLPVLDMIAKMLTVKAHNIWLTSGD
                     PKRQKTAVEYVLKGFESGALKPVIARTFRFDEMVDAHRYLETNGQFGKVVVTV"
     gene            complement(14603..14947)
                     /locus_tag="VHV99_08205"
     CDS             complement(14603..14947)
                     /locus_tag="VHV99_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX3635456.1"
                     /translation="MQTNAIRRAFFVLLGSSMLIAAPAGMSVARAETPGAVVIKNFMF
                     SPMALTVKTGSTVTWKNLDGEPHTVVNDAGMFRSAALDQNDTFQFKFDKPGVYKVFCG
                     IHPNMKETITVQ"
     gene            complement(14982..15950)
                     /locus_tag="VHV99_08210"
     CDS             complement(14982..15950)
                     /locus_tag="VHV99_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX3635457.1"
                     /translation="MSSHHSSDPAFDPAFNQARRGALKCLAFGGVGTVFVLAGGILTP
                     VELALAADAKSSSATTGVPLFLQISDSHIGFNKEANPDVAGTLKQTIEYVNAMPVKPA
                     LTIHTGDITHLSKPAEFDLAAQLMSGLNITELHTVPGEHDVTDGPGTEYFSRFGKASD
                     NKGYYSFDHNGVHFVALVNVMHFKPNGLGGLGDEQLAWLENDLKGRSSSTPIVVFAHM
                     PMWTIYEPWGWGTGDAGQAMSYLKRFGSVTVLNGHIHQIVSKVEGNITFHTARSTAYP
                     QPTAGNGPGPGPLTVASDQLPKMLGVTSIRVVRHPLHATLADTTLV"
     gene            16181..16372
                     /locus_tag="VHV99_08215"
     CDS             16181..16372
                     /locus_tag="VHV99_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007743880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635458.1"
                     /translation="MDIIDMARASGITVILDGRIGREEYQSVCGSLSALQRFAEAVRQ
                     GTVQQSDNQKRARTAARLG"
     gene            16464..17198
                     /locus_tag="VHV99_08220"
     CDS             16464..17198
                     /locus_tag="VHV99_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635459.1"
                     /translation="MQPALRRLQLGSGVVLLIYLFLHLVNHALGIWSLDLAGRGLTFA
                     LWIWHSVPGTIALYGSALLHFALAVRTIYGRRHWTLPPAEWIRLWAGLSLPLLLIRHA
                     VATRLAASLYGFEPNYERIVISLIVSGTQGLQLALLAPGWIHGCLGLWFRLRHYPAAQ
                     RASPVLLAFLILLPLLSAAGFLRMEQAVVAMHAVLPAPDSTLLAHQLALDMWRHSLVT
                     LYLVVLVGALLAGLLRNWLERRLRAA"
     gene            complement(17444..18442)
                     /locus_tag="VHV99_08225"
     CDS             complement(17444..18442)
                     /locus_tag="VHV99_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX3635460.1"
                     /translation="MAIPSNHDARRHGRRRFLRTAGSAAIGLPVSFALPHITPAQGQS
                     QTQTQTETTTTPLPRMAQRVIPSTGERLPVVGCGTWRTFDVGNDAAAQSQLADVLRIL
                     FEAGGSTIDSSPMYGSSEAVAGELLTRLDAHKKAFVATKVWTEGRAAGIAQMEESLRR
                     FQQPRIDLMQVHNLLDWRTQLATLRDWKARGRIRYIGITHYTSSAFGEVAAVMRSEKP
                     DFVQINYAADDRDAEQRILPLAAELGIGVVVNQPFGGGGLLARVGKQALPGWAAEIGC
                     TSWAQVLLKFVLAQPAVTVVIPGTGRPQYMADNVRAGSGPLPDKAMCARIVSTVNA"
     gene            complement(18728..19063)
                     /locus_tag="VHV99_08230"
     CDS             complement(18728..19063)
                     /locus_tag="VHV99_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX3635461.1"
                     /translation="MAKSPVSQLDRTFSALVDPTRRAILARLEREPGLSITEIAGPLP
                     LKLPAVMKHLDVLSDAGLVARKKNGRTVSVDLIAGPMEEAMAWLRHYERFWSASVDRL
                     ADFVEGQVE"
     gene            19200..20117
                     /locus_tag="VHV99_08235"
     CDS             19200..20117
                     /locus_tag="VHV99_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007731880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX3635462.1"
                     /translation="MDILQGMAVFKRVVDASSFSKAADSLEMPRASVSTLVQNLESHL
                     GVRLLNRTTRSVRVTDDGALYYEYCARILAEVSDAESALSNKLQNPRGTIRVDTSATF
                     ATNVLLPMMRDFNARYPDITVKLGLADRNVDLVQEGVDCVIRIGTLDDSSLVARPIGS
                     VRMCICAAPAYLEQMGEPATPDDLDRHRAVNYFSARTGRVYPFEFKVDGTLIRKPVDG
                     ILGVNDGLVYVMSAIEGLGLIQTPRFMAAQALRTGALREILRDYPCPPMPLSLLYPQR
                     RLSMCVRVFSEWVHELALNNPDLNGEADA"
     gene            complement(20186..>20782)
                     /locus_tag="VHV99_08240"
     CDS             complement(20186..>20782)
                     /locus_tag="VHV99_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012405805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX3635463.1"
                     /translation="DAFEKGAKEANGNVVDREFTNDKAVDFRAILTTLKSKNVDLIFF
                     GGLDEQGAMLVKQMRSLGMTAQLFGAGALKSNAFLQIAGTAGDGTQDLEPGPALDKLP
                     AAQAFGKRYKARFNQDVELYAPFAYDAALAMIKAIHGADSLDRAKIVDSLSKVKVTGV
                     TGDITFDPYGDLIKPPYTLFQVQQGQWKSIKTVGGNGV"
BASE COUNT         4024 a         6389 c         6249 g         4120 t
ORIGIN      
        1 agttttcaat attgcgagtg ccagattcat atcgcgtttc acgatctcct cctgtcgcag
       61 ataggacata catcgaaagg gcgccacggg tacggcgtcc gcgcgatcga tgtgattccg
      121 ggttcccaac aggctcatgc cacgatccga acgcgtaggg tgaatttcaa caccagtcgc
      181 gagcgctgcc tgggattcaa cgcttcggtc ccctcatacc ctcggtttcc atcgcagcat
      241 tccgctcttc gcaggcttca atgggttcca tggtctcgtc gatggaagct gtcgcgcgtt
      301 ctgtggccgc cttcgtttcg tcgacggtgg aaccgagtcc tccgtcgtcg tcgctcgggg
      361 gctgcagcat gtctcgaagc atggcctcca actccggcgt atcccacggc gcgccatgct
      421 gctttttctt ctttatgtag tgtctgactt ctgcgtcctg ctccggcgtc aggggaagac
      481 cgcgaaatgt gctttggggg gtagcgtcgg tcatggtatt gctccggaat ttatcccatt
      541 ttgagtgtag ccctgttgtg gccaacgagc ctctgctatc ggtctgcgca tggactgatc
      601 cgtccattcg catcggctcg tccagcgagc ggacgttgca atcggcacgg atacgagccc
      661 gggcgctttg cgcaccgatg caaattgcga agggtcatga aggatggcag aaactatgac
      721 aaaactgttc ggacagtttt ccatcgagcg cgtgcacaaa agctgtacgc tggtgattcc
      781 cgactgcgac aggatcggac ccagatgagc ggatggcatt cggctttcac aacctcagcc
      841 gcagctatga cgcactcggg cactgcgtaa cgttctgggg ttatgacaac gtcgtgaagg
      901 ggaccacgaa tatcggtgtc ggacagacgg gagggcggaa gatgcgactg atcgaacggg
      961 atgaacgcca ggctttcctg gcgaccttgc agcgcaaggg actggcagaa ggtgaattcg
     1021 aactgctgga aacggatacg actgacccca agggcgacga gaacgtcggc ctgcagggat
     1081 atgtcaccgt aaccagacta tcaacacaaa ttgcgaggga ataccccatc ggcttcgaaa
     1141 ccggttgggt gcagcatttc agacaggatc tcgaagctgg cgtcttcgat aagccggaat
     1201 gagatcccgc tgttgcggcg aactctccga gcggagcaag caggtatggc gaaatggaag
     1261 ccggatcgaa cgggatcgag gccacggaaa actcgggacg cagacctaag attcgcatta
     1321 ccgcaagcct ccgggatgac cagtccgtgc tgctcgaggt catcgataac ggttcgggca
     1381 tcgtaggtga cgacagcata ttcgatgcct tcgtcacgac caaaagtaaa ggaatgggta
     1441 tcggacttgc ggtgtcccgc tcgatcgtcg aggctcatga aggccatctg actgccgcca
     1501 ataacgatgg ccatggagca accttcagcg ttgtcctccc cgtatcgccc ttaccgattt
     1561 cacagcaggg ggtggggatc caataacctg gccgggcgag ttgatccaca gtgtgggccg
     1621 ctgcctcagc tccctttcca taaggtcttt cctgtgcact cgcaactgtg attcttgtca
     1681 gggcggcctt cgcaaattac cgagtggggg ctcacccaga cgcccctctt catggcgtgc
     1741 cacgcacagc cggcgaccgt gttcgaaggc accagatagt aagggtcccg gacacattgc
     1801 aggttcttga tgtgggtcaa gccgtcgctt tctttcctga tcaccaggca tgtcgagtca
     1861 gccggatcca cgggcgttcc agcaacagat gccgggaccg ccccagccga gccgctcgac
     1921 ttctgattgg ctccggaaag gtcaggcgcg ccctgacttg tggcagcggt gctcggagtc
     1981 tggcttccga ttgccgagtc caggctttta tgggcggcgt cggctagtcc ctgtttgagc
     2041 gtctgggtga gatcgtcaaa ggttccggct tgtgcagtac cgagacaaag gaggcaagcc
     2101 aggccggcgc ataggcgaac gaaacgtgta ccaggaaaac tcttcgagct ggaaatcatt
     2161 gcgtttctcc ccatggtgtc cgtcctggtg ttggcgggtc gggtcagagc ccgcaagcct
     2221 tcatcgaagc gcgtcgcaca gcctccgcct tgcgcaggtt cgggtcgggg gcgcttagtc
     2281 tgccagcggc atcgtattca gatcgatgtt tgtaccatcc gggcaagcgc acggcaccgc
     2341 gtagggcgat gttttcggca acggcattct cgtgttgcac aggcgggcga ccggcattgt
     2401 gctcttcacg ccatgcgccc gcacccacga agcgatgcac attccgttgt tgaccctgcc
     2461 ctcatgggtc atcacggtca gctcgctgtc gcagtcattg acgatggtca tgatcatggc
     2521 gtcgttccgg acaatgaatg ccttcattgg gctcgagagc cgcctctttg cgcaaccggc
     2581 gggcgcctca cccgtcgcct gagaggaagc gcccggagcg ttcccgcgga ccggctggat
     2641 cgcgtcggcc gcctggggct gtgcgggtcc cgtgccggcc gggccggagc tctgcacctg
     2701 cgcgggactg cctggcggcg tgttcccgag cgctgcgtcg acgttcttct tcgccgcatc
     2761 gttgaggctc ttcgccaggc tttgcttgag gtcgtcgaag gcgccggcgt acccgtacga
     2821 tgaatgcagc gcgcagccga gcgtcaccaa cgcagcaccc gcgccgaaga gaaatcgacc
     2881 ccttgacctc gtcatatgca actccagcgt catctgagga gattttgcga gcgatcaggg
     2941 gaccaccttt cctgaccctt ggctttcagt ctagggcgtg aagcgcggtt ggtcgtctct
     3001 caaatgggag agaaattgtc gacggatgga gatggcagcg acttctacat tcaaagcagg
     3061 gaggaccaac taacgctgtt tctttgcacc gcggatcccg cgcgtttatt taatattggt
     3121 tcgccggtgt attacgccgg acgaattatc aaagtgagtc atagtggcgg cgatcaggac
     3181 gttgatggct ttcgcgtcgc ggctttggat gtggggcggc gaggtttagg cgtctcgtca
     3241 gaagatcgcg gacgcttggg tggcgccgcc tgattcgttt tgacggtcgc caactgcagt
     3301 cgcaattcgt tgagctccgt gacatatgcg gtgttcgaat ggcgaagggc ttcaagactc
     3361 ccttccacga cacctgcgcg atgacgcgca tcgccaagct gaacctgtaa cgcccggatt
     3421 tcctcccgat gccgggtctc gccctgttcc gccctgcgcg ccgcggcagc gagatcctta
     3481 tggaggcgca cgccggtgct ccgctcgcgt tcgatctcaa gcaatgcccg tttctcagcc
     3541 gcccgcagac gctcctccgc cagctcagcg ctaccgcgca acttttccag ttcacccgaa
     3601 aaatccgccc tcgcctgcac cagtgcgcga tcgcgcgcgc cgatctcgtc ctgctgccgc
     3661 gcgacttcag tctcgagcgc ctgacgcgtc gcctcgcccg cagcttgcgc ctgctcgagt
     3721 tcctgaatcc gcacttgcgc cgtaagcaag gcgccggtgc gctgctcgag cgccgtttcg
     3781 gttcgcgcga gttcggcccg caatgtctct accacattgc gcgcggcggc cttctccgct
     3841 tcggcgtcgg cccgcagttc ctcgagcgcc gcttgcgccg acgtggtgga cttcgtccac
     3901 agcgtggcga tcagttcccc ggctgccgcc tgcagatccg caggtaagtc cggatgctcg
     3961 atccgtaccc tgcttttttc gcgcagctca acccagaact cgccaagcac ctgagtcggc
     4021 gtgctcatgc tgccgcgctt caccagttga tacagccggt ttgcggtcgg cgtaatgccg
     4081 aagcggaaga acaacagcgc gcagacctcc cgatacaact cgcgggtttt cgggaattcg
     4141 gccttcagac gatcgatctc accggcgagg cgcgcttcat caggcaggac gttgaccatg
     4201 ggaatgatgt gaggtttgat ttagcaaata atataacgta aatcgctaaa tatccaacca
     4261 ccatctagct taatgttggc gttacgggtg caacgtcgcg attgcgcccg ctggccccgc
     4321 caccgcctgt ttgcgacaac cacatgtgga aatgcgacac gggcgccgcg cgggcgcccg
     4381 cagaagatgg gcaaactctt acgggacaag ggcttgcagg gtgctgagga agtgggttgc
     4441 gcgtgtcgcg ccagagcaag cgggagcggt ttggccgaaa aacggctgcg ttcaggcagt
     4501 agcaggcgct tcgccgcaac gcagcggcgc aggtcgctcg caggtgccgt tgcgcacccg
     4561 cgaggcttat caatccaaaa gcggcaaact cattgcggcg cgggcgccgt ccggtatcct
     4621 cgccggccaa cagccaaagc aagcatggcg gacaccgcaa gactcgccgc taccacatac
     4681 agcccggcag catagccgcc cgtcacgctc ttcagccagc caattgcaaa cggcccgacg
     4741 aaaccgccaa gattgccgag cgagttgatc atggcgatac cggccgccgc gccggcgccc
     4801 gagagaaagg tgctcggcat ggcccacagc ggcgccttgg ccgcgctgat gcccacgttc
     4861 acgaggatca gcgcgagcac caccgcaacg acggtatccg caaagcctgc ccacgcgagc
     4921 ccgacgcccg cgacaacgca tggaatcacc acatgccacg tgcgctcgcc agtgcggtcc
     4981 gaatggcgag cccacatcac catgccgacc acagccagca cgctgggaat tgcattcaat
     5041 gcgccagtgc caagcgcgct aaaaccgaat tgacgaatga tcagcggcgc ccacagcccg
     5101 agcgtataaa gtcccgcgga cgtgccgaag taaatcatcg acatgatcca cacgcgcgga
     5161 tcgcgcagcg ccgcgagcgc gcctgtcgcg tgcccggcct gggactcgcg tcccgcccgc
     5221 tcttcgcgca aggtcgcgat aagccatgcc ttctcatccg ccgcaagcca cctcgccttt
     5281 tccggcgaat cggtgagtgc tttcaaaacc acgaatccga gaacgacagc gggaatcgct
     5341 tcgatgagaa acagccactg ccagtctttc aaaccgaaca gcgtcggcat ctgcatgatg
     5401 gcgcctgaaa tgggcgaccc gattgccgtc gacaaaggcg cggccgccat gaacacagcc
     5461 gccgcgaccg cgcgctgccg tgtgggaaac cactggctga gatagagaat gatgcctggg
     5521 aagaaacccg cttcggccag cccgagcaag aaacgcagcg cgtagaaaga gttcggtccg
     5581 acgacaaacg cggaagccgc tgacaccagt ccccacgtca ccatcacgcg ggcaatccag
     5641 atgcgtgcgc cgaccttgtt gagaatgagg ttcgacggta cttcgaacag aaagtacccg
     5701 agaaaaaaga tgccgccgcc gagcccgaac atggtcggcg agagacccag gtccttgttc
     5761 atcgtcaggg ccgcgaagcc gacgttcacc cgatcgagaa agctcacaaa gtaaagcagc
     5821 atgatgaagg gcaagatgcg ccacgtcagc ttgcgaacca cacgggcttg caattcattg
     5881 gtcatgcctg tctccgaact ttagatcgcc cgtgacgtgt tcgtccgggc gttatgtttt
     5941 tttgtcacgc acgcgctgtg ggttgcgcgt agcgtgtttt gccttgcgta tggcgtgatc
     6001 aggctgcctt gaattgcgcc gctggttccg ctgcgagaat tgcacatacc gcatcggtca
     6061 cctgcacggt cgtggccgtg ccgccaaggt cgccggtgtg cagcgccgga tcagccgtga
     6121 tgcgctcgat tgcgttcatc aattgttggg cggcggccgt ctcgccaaaa tgttcgagca
     6181 tcatcacgac cgaccagaac gtgccgaccg gattggcgag tcctttgccc atgatgtcga
     6241 aagcggagcc gtgaatcggc tcgaacatcg agggatagcg gcgctcgggg tcgagattcg
     6301 cggtcggcgc gatgccgagg ctgccggcca atgccgctgc cagatcgctg agaatgtccg
     6361 catgaagatt cgtcgcgaca atcgtgtcga gtgttgcggg acggttgacc atgcgggccg
     6421 tcgcagcgtc gacgagttcc ttgtcccact tgacgtcggg aaactccttc gatacttcca
     6481 gtgcgatctc gtcccacatg accatcgcgt gacgttgcgc attgctcttg gtgatcaccg
     6541 tcagcagctt gcgaggccgt gactgtgcga ggcggaacgc gaaccggaga atgcgctcga
     6601 cgcccgcgcg ggtcatcatc gaaacgtccg ttgccacttc gatcggatgg ccctggtgcg
     6661 cgcggccgcc gacgccggag tactcgccct ccgagttctc gcgaacgatg acccagtcga
     6721 gatctttcgg cccgcaacgc ttcaacggtc cgtcgatgcc aggcaggatg cgcgtgggac
     6781 gcacattcgc atactgatcg aagccctggc agatcttcag gcgcaaaccc cagagcgtca
     6841 catgatcggg aatatgcggg tcgcccgccg agccgaacag aatcgcgtcc ttgttgcgaa
     6901 tggcatccag cccatcggcc ggcatcatca cgccatgctg acgataaaaa tcgccacccc
     6961 aatcgaagtt ctcgaactcg aatctgaagg cgtcgctgcg cgcggcaatg gcttcgagaa
     7021 cccgttggcc cgcgggcacg acttccttgc cgatgccgtc gccgggaatg gttgcgatgt
     7081 ggtaggtctt cacaggggtc tccatgacgt taaacgtagg gcagtatcct agaacacgga
     7141 ctgcgcgcga aaacgctgct agacttaaag catccttaac cttgggttaa taatggacgg
     7201 ctcattggac tgctcatggg cttctcctgg tcttctcatg ggctagtcaa gaactacccg
     7261 agggttaccc cgccggctac tcagcgccct gctcaccaag ctactcaacc gccgcctcaa
     7321 catgacctcg tcgatacagc cggacgacct cgccttcttc tccacgctcg ctacaagcgg
     7381 cagcctgacg gcggctgcgc gtgaactggg gttgactacg gcggccgtga gcaagcatct
     7441 gtcgcaaatg gaatcgcggg cgggcgtgtc gctcgtcaat cgcacgacgc ggcgcatgag
     7501 cctcacgccg gagggcgagc tctatctgac gcgcgcgcgg cgcattctga gcgaaatgga
     7561 cgacctggcg cagttgcttg gcggctcgca agcggcgccg aaaggcctgc tgcgcgtgaa
     7621 cgcgacgctc gggttcggcc gcagtcatgt ggcgccgctg atatcccgct ttgtgcagcg
     7681 ctttcctgag gtggacgtgc aattacagct ttctgtcgac ccgccgccga tcagcgacga
     7741 cgttttcgac gtttgcgtgc gcttcggcga accgccggat gctcgcgtga ttgcgcgccg
     7801 ggtcgcgtcg aaccggaggt tgctttgtgc atcgcccgcg tatttcgccg aacggcgcgc
     7861 accacgcagt ccggctgagc tatcgcagca caactgcatc ggcattcgcc agggcgatga
     7921 tggctacggg ctatggcgtc tgaccagcgg acgcggttcg accgaaaaga ccgagacagt
     7981 tcgcgtgcgc ggcaacctca ccaccaatga cggcgagatc gcagtgaagt gggcactgga
     8041 cggccacggc attttgatgc gggcggaatg ggatatcgtg gagtatctcg ccgatggccg
     8101 cctgattcag gtattgccgg aataccgcac gccgggtgcc gatatttacg cggtgtaccc
     8161 gcaacagttg cagatgactg cgcgggtcaa ggcgttcgtc gacatgttga tagaggcgct
     8221 gggagcgcgt tgagtcatgg catggcgagg cggctggggc gcgtctcttg ctctcggtgg
     8281 gcgcggccgt gcgcgccgcg gcctctttct gcagttattg atcatgggga tttgcggtat
     8341 tggtctttcc ttgatctaac tctttggcct ttccttgatc tggctttttg gcctttcctt
     8401 gaactggttc tttggccttt ccttgaattg ttagtggtct attagtgtcg cccctgtgcg
     8461 gggcaggcac ctacttttct ttgcctgccg caaagaaaag taggcaaaag aaagcggctc
     8521 acaccgccag cgcataagcg ggtcccccgc acagttgcgg tagtggtgca tctggaatct
     8581 gtgttctcgc acattcggcg tgagtgacac agcagtcata cttccggcgg cgctgcgcgc
     8641 gccgaagcgc atttcaaaaa aacactacta ccatttcaat cacacgttcc atccggtatc
     8701 gggctcgccg ttcaccgcta cagctacaac aattacgacg gccacgctgc gcgccgaaga
     8761 gcggttcata gactcgacca ccccattttc gctctcgagt tctgcttagc actgcggctc
     8821 gccgctggat tgccaccgac atggcaacga ctgctcctgc cattgcaacc gtatgtaccc
     8881 gcagtcattt ggccggtgtt ttcgcacttt cattcgctag cggagtgcgt ctttacgata
     8941 actgtgcgtt tcgccgaggc gaagccaacg gctcccccca agcgcaaacg gagcccatgg
     9001 tttcccaggc agactcatcc gcgacgcacg tagtgcggag tgggagctga tgacagcctt
     9061 gtcactgacg cggaatgtgc gagaacaccg attccagatg caccactacc gccactgcgc
     9121 gggggacccg cttatgcgct ggcggggtga gccgctttct ttgcttatct ttctttgcgg
     9181 cggcaaagaa agtaagtgcc gccccgcaca ggggcaacac taatggacca ctaacaattc
     9241 aagaaaaggc caaccccgta aaagcacaga cgaacaagca ccgcacagac acccacctaa
     9301 agcaaacccc aacaccgcag gcacccaacc cctagccccg cgaagacaat ccgaacagcc
     9361 aaaacccaaa agtcaaaatc tacacacaaa taaaaaaaac atccgaccgc gcggcgtggc
     9421 tggaactcat catctctctg gccaacacgc tgctgccctg catgcgcgac tggccctacg
     9481 cggacgtcga cggcgatcct gccggcgccg aagcagtcag ggcgctcgcg cggcaccgta
     9541 tcgaagaagc ctgtgaccgg ctcgacacca atctcgctga cgaccgtgat tgtctggtcg
     9601 gcggcacgct cagcaccgtc gattttctcg ccgtcgtgct gatgcgttgg actcgcaata
     9661 tgccgcgccc cgcattgggc tggccgcatc tcgcccgcta tattcgcgga ctgcgcgccc
     9721 tgccctcgtt catcgaactg aacacgcgcg aggggctcac gcgaatggcg taaccacgac
     9781 gattgatgcg cgctcaacca attcgccccc gacgctggca agcgagacga acacgccatg
     9841 aaagccgcca gtcagttcgc tcgcgtgccg acgaccacaa tgccgcgtga aagtccgctc
     9901 acgttatcga ccgcctgggc cgctgcgtac agttgcgtga gcggcaccca caccgccgga
     9961 tacttgtagc gcgcaacatc gagaagcagc gcgctgtcgc ttgcggcatc gtacgcggca
    10021 agcggcgacc agtgaccacc gccggcttca ccgatctcca cccgtctgaa gttcaatagc
    10081 gcaaagcggt cactgtggcc ggtcgtgtcg cgaatcagat cgcgaaactg ggcgagcgtc
    10141 agatcagcgg cgtggaattt cttcacctcg acaggaaacg cactcagggc cgcgctaagc
    10201 tgatcgagcg tcatgccctc cctcgatacc cgcgcggcgt tggccacctg cggggcgacg
    10261 ttgcgaaaga aatcctcttg tgagaagtat gggaaatccg ggtactgcgt cgactcggga
    10321 cggcggatac ccaatgcgtt gagcgccatc accgaggtcg cgaccgaaca gtaggcttcg
    10381 ttgcgctgcg tttcgaaata ctccgaaagc ggccagtacg actggttatg gggggtggct
    10441 atcagacgct tctgacccgc cggctgcgtc aatgcaatga ggttaggcgg aaccggcagc
    10501 ggcccgtcgg ccgggcgtgc cgcgcttgct gcgcttgctg cggggacctg atacgaatcc
    10561 tgatgggcag tttgttccgc gacttgattt gctgccggcg gcagctgcgc acaggccgcc
    10621 agggttgccg tcagaaaagc ggtcagccat ccagatgggc tcatacgtcg cacggcatca
    10681 atcaaggaat atctggacat tgccctttcc gcagtggatg agtttcgaac gattgccatc
    10741 ttccaatgct tcctgcgcat cggcgacgcc tcgaattttt aacacgaaac caatcgacgc
    10801 gtgagtttgc gtcccagccg tgcgtttgtt cggccgcttg ctatactccg gcggcctatc
    10861 ctggcgcgcg cgaccgaaca gcttatcgaa gtcgctcaca gcaaattcaa gtagaccgga
    10921 tccgaatatg agtgacttaa aaagcgccgc gtcgggcgca ggacgcgacc ctgcctcgct
    10981 ggacacttcc gacaaaaaac aatgccaagc ccacggaccc gatgcgccga ttgcggcagt
    11041 gccttctcct tgcattgacg tctgcaaaat cgatggaaat acgggcatgt gcatcggttg
    11101 tctacgcaca cgcgacgaaa ttcgcggatg gaaaaacatg accgatgatt tgcggctcga
    11161 gatgatcgag gaactgtcgc accgtaaggc gaatctggaa gcgagcaagg caagaatgct
    11221 atagagtcgg caccgttgag cgcacggatc aactcacccg ctcagcgaac gcgtaactgt
    11281 tcttgccgct gcgcttgacc cgatacatcg cctcatcggc cgcggtcacg aggctgtggt
    11341 aatcgccaac gggatcagga aaactggcga tgccgatgct ggcacgaacc aacccgttgc
    11401 ccatctcctc gtccgtctga accacacagt cgatcagtcg ccgggcaatt tcgcccagct
    11461 ctgcattcgt cgaaaattcc cggatcagca cggcgaattc gtcgccgcca atccgcgcga
    11521 cgacatcgac tttgcgcacc gactcacgaa agcgtttcga aaccgcaatc agaaattcat
    11581 cgccaacgcg gtgaccgagc gagtcgttga cctgtttgaa gccatccaga tcgacgtaca
    11641 acacggcgat ttttcgaggc gacccgcccg cgagcgatcg ggtcgccgct gcttcgagcg
    11701 tcgcaaacag cttgcgccgg ttcggcaagc cggtcatcgc gtcatgcagc gccgcttgct
    11761 cgaattcgcg cgactggccc gcgagcaagc gattccgttt ggcatacatc cccagcgcgg
    11821 taatcagcat gcagaacagc aacgctgccg acgcgatcag cgtgtgcgcg acgtaggtaa
    11881 tccgtgcgcg tgcatcggcg ctggcagcgt cgcgctgcgc gcgccacccg ttcgacacgg
    11941 cagtgagcca ggcatctgtg tcgcgcagat cggcatcggg tgccagcgcc gacgaagaca
    12001 catgcggcgc cgccgacgga cttgcattca ccagtgccgc cagtgacgtg aggcgacggg
    12061 cgagttcggt gcgcgcatgc cgatacccgg cggctcgcgt tgccatggcg tcgggcaatt
    12121 gctgccagat gccgactacg ctgcgggcgc tttccgcccg ctcgaccgcg tcgagaacga
    12181 gctccgcata ttcctgcttc aactgatcgg agaagatcgt cctgatttgc agcgcaaggt
    12241 atagaaggcc aatcagcaga caaaccagcg cggcggcggc gatcgcagcc ggccctggcc
    12301 tgaagcggat cgcgagggcg cgcccgcgag cggcgcgcga ggtacgcgcg cgctcaggga
    12361 tgggaataga tgtcttgccg gcttccggtg ccatgctggc gataacttac gcggcgaaag
    12421 aacgaaccca ttcgggcgaa caggctgccc ttgcgcctgg catcggccgt cgaagaggat
    12481 cggcgatcct gttgagcgag acgcgcatcc ctggccgctc gactggacgc cagctgcggt
    12541 tgcgcaacgg tattggaggg gggcgccgcg cggtgagccg caggggcgga agatggggcc
    12601 atggcgggcg ttgcgtcggc cacgctgatt tggcgagccg ccggcttagc gagttgcgtg
    12661 accggacccg cccaggaggc ttgctgtttt cctgcttcgt ccggtgctgc cgccgtggcc
    12721 gccgggcgcg acgctgccac ggcagtcgcc acctttgcag gcgcaatcac tttcgcggac
    12781 gccggcggtc ctgccgtcgc cagcgcagcc gtcccgagcg cctgccgtgc gcccgccatg
    12841 gcgtcggcat acgcctgctg atcgtgattg aaccagacgc cgtaagcgac cgtacccacc
    12901 acgccaaaag tcagcgcgct ggccgccgcg acgcacagtg caagacgtcc gaatcgtacg
    12961 ggcggtgccg cgtgcggtgg ctcagcgcca gcgtcatcgg cgacatccgg atgctctaca
    13021 gcaaatttag gtaggtcggt gtcgttgaac attgcactac tctccggaat ccttacgtct
    13081 gacatgtcgg cagactttaa tcacacgatt cagcggtggc cgttctggag ttattgaaga
    13141 tttaacccgc gccccgggta gcgacaatac ggcaatgctg cgcttaaaat ggccgctttg
    13201 cgagccgtgt gtgcaccaat aaatctagta aaaaattcaa cgccgcgtgc atcgacgagt
    13261 cgtgccggga ggccttgcag gtcgcgtttg tacgccgggc gtgggatggt gacgtgagca
    13321 tgcgctggtc gctatagagc acgcaacaaa cacgcccgag gaaaccgggc cgacgtcgtc
    13381 gctttcagcc gccacttttc gtcgaattcg gactttccgt atcgtgctga aacacaagtc
    13441 gccactcgtg cgttacgtgc gcatcgaatc ctcagaggag cagaaaaaca ttactcggat
    13501 ttcccggctt attcgtctac tgtagaaatc gccgcgaccg tgcgagtcaa ggcactctcg
    13561 ccccgctcca accctgctac caaccgtgga gttttcatgt cacgcaccat catgttcgaa
    13621 aaagccggtg ggcctgaagt gctcgaattc gtcgagacgc ctgtcgcggc gcctggtccg
    13681 catgaggttc gaatcaaggt cgaggcgatt ggcatcaacc gcgccgagtc gatgtggcgc
    13741 aatgacgcgt acatcgaacc cgtcagattt ccagcgggac tgggctacga agccgcgggc
    13801 atcgtcgacg cggtgggcgc cgaagttcag ggggtcgcgc ccggcgacaa ggtcaacgtc
    13861 atgccgtcct tctcgatgaa cgagtacttc acgtatggcg aagtcatcct cgtaccggac
    13921 tacgcggtcg tgaagcatcc cgagtcgctt tcgttcgccg aggcggcctc ggtgtggatg
    13981 atgttcgtga cagcctatgg cgcgctcatc gaagacgcca aagtgaccag cggcgatttt
    14041 gtcatcgttc ccgcagcctc cagcagcgtc gggctggcgg cgatccagat cgccaactac
    14101 gccggcgcga cctcgattgc gctgactcgc atgtcgggca agcgtcagca actgctcgac
    14161 gccggtgccg cccatgtcat cgccacggaa gaaaccgatc tggtcgccga ggtgatgcga
    14221 atcaccgacg gcaaaggcgc gcgcgtcgcg ttcgacccgg tgggcgggcc gaccttcgcg
    14281 aagctgattg cagcgttgtc gtttcagggc accgcttaca tctacggcgc gttgagcgat
    14341 gaggtcacgc cgctgcccgt actggacatg atcgctaaaa tgctcaccgt gaaagcgcac
    14401 aacatctggc tgacgagcgg cgatccgaag cgtcaaaaga ccgccgtcga atacgtgttg
    14461 aagggttttg aaagtggcgc gctcaaaccg gtgatcgcac gcactttcag gttcgacgaa
    14521 atggtggatg cacatcgcta tctggaaacc aacggacagt tcgggaaggt cgttgtgacg
    14581 gtgtaagcgg catagagaaa atctactgca cggtgatggt ttccttcata ttcgggtgga
    14641 tgccgcaaaa caccttatac acacccggct tgtcgaactt gaactggaat gtgtcgttct
    14701 gatcgagcgc ggcggagcga aacatacctg cgtcgttcac gacggtgtgg ggctcgccgt
    14761 cgagattttt ccacgtcacc gtcgagccgg ttttgaccgt cagcgccatc ggcgaaaaca
    14821 tgaagttctt gatgaccacg gcgccgggcg tctctgcccg tgcaacggac atgccggccg
    14881 gcgccgcgat aagcatcgaa cttccgagca gcacgaagaa ggcgcggcga atagcattag
    14941 tttgcataac ggatctccaa ttgattaaat aaaaacctga atcagacgag tgtcgtatcg
    15001 gcaagcgtcg catggagcgg atgacgcaca accctgatgc tggtcacccc gagcattttg
    15061 ggaagctgat cgctcgccac cgtgagcggc ccagggccgg ggccattgcc ggcggtcggc
    15121 tgcggatagg ccgtcgagcg cgcggtatgg aaagtgatat tgccttccac cttcgacacg
    15181 atctggtgaa tatgaccgtt caggacggtc accgagccga agcgtttcag atagctcatt
    15241 gcctgaccgg catcgcccgt gccccaaccc cacggctcgt agatcgtcca catcggcata
    15301 tgcgcaaaaa cgacgatcgg cgtgctggac gagcggccct tcaggtcatt ttcgagccag
    15361 gcaagttgct cgtcgccgag gccgcccaaa ccgttcggct tgaaatgcat cacgttgacg
    15421 agcgcgacga agtgcacgcc gttatggtcg aagctgtaat accccttgtt gtccgacgcc
    15481 ttgccgaaac ggctgaagta ttcggttccc gggccgtccg ttacgtcgtg ctcgccgggc
    15541 acggtgtgca gttccgtgat attcagcccc gacatcaact gagccgcgag atcgaactcc
    15601 gccggcttgg aaagatgcgt gatgtcgccc gtgtggatag tcagcgcggg cttgaccggc
    15661 atggcgttga cgtattcgat cgtctgtttg agcgtgcccg cgacgtcggg gttggcttct
    15721 ttattgaagc cgatatgcga atcgctgatc tgcaggaaaa gcggcacccc cgtggttgcc
    15781 gaggatgatt ttgcatcggc cgcgagggcc agctccacgg gcgtgagaat gccgccggca
    15841 agtacgaaca ccgtgcctac gccgccgaag gcgagacatt tcagtgcccc gcggcgcgct
    15901 tggttgaaag cagggtcgaa ggcaggatcg gatgaatgat gtgacgacat gttgacctca
    15961 cgaaaaggat tcgcaaacaa ccgcagggtc atcccgcggg catcgcgcct cggttcatga
    16021 gcaagactgc cgcaccatcg gttttattcc aggcgcggaa aaatagcatc ggccagggag
    16081 tgccgccctc gggcttatac gtctcgtgtc aggatcaatg tgggaataac cgcgctgcgg
    16141 ccgcagtcat gcagaccagg aacctgaaag gagtagcacc atggacatca tcgacatggc
    16201 ccgtgcttcg ggcatcacag tcatcctcga cggcaggatc ggccgcgagg agtaccagag
    16261 cgtgtgcggc tcgctatccg cattgcagcg attcgccgaa gcagtgcgtc aaggcacggt
    16321 gcagcaaagt gacaatcaga agcgcgccag aacggctgcg cgcctcggtt gagacccgac
    16381 cggcgacgca gcgctgcttg aattcgctca atcgccgcgt ctgaaacaga ccttctgaac
    16441 atacggtttt cacggaggaa gatgtgcaac ccgctttacg ccgcctgcaa ctcggatcgg
    16501 gcgtggtgct gctgatttat ctgtttttgc acctggtcaa tcatgcgctc ggcatctggt
    16561 cgctcgatct tgccggacgc ggactcacgt tcgcgctgtg gatctggcat agcgtgcctg
    16621 gaacaatcgc gctgtacggt tcagcccttc tccatttcgc gctggccgtg cgcacgattt
    16681 acgggcgccg ccactggaca cttccgccgg ccgaatggat tcgcctgtgg gccggactca
    16741 gcctgcctct gctgctgatc cggcatgcgg tggcaacgcg tctcgcggcg tcgctgtacg
    16801 gcttcgagcc gaactacgaa cggatcgtga tatcgctgat cgtgagcggc acgcaaggct
    16861 tgcaacttgc gctgctcgcg ccgggctgga ttcacggctg cctcggcttg tggtttcggt
    16921 tgcgccacta tccggcggcg caacgcgcga gccccgttct gcttgctttc ctcattcttt
    16981 taccattgct gtccgctgcg ggttttctgc ggatggagca agccgtcgtc gccatgcatg
    17041 ctgttctccc cgcgcctgat tcgacactgc tggcgcacca gcttgcgctc gacatgtggc
    17101 gtcatagtct cgtcacgctt taccttgttg tgctggttgg cgcgcttctg gcgggtctgt
    17161 tgcgcaattg gctcgagcgc cggctgcgtg cggcgtgacc cgacggctgt gccaatttcg
    17221 tcgtttcagt tgggactttg ccgtgaccac ggtctgattg gtcgtcagcc caactggtag
    17281 cccatttatt gaatgtagcc ttggaagcta catgcaggaa agtcgcattt taaggctgct
    17341 tttctgatag tagcctctaa aggctacgct accgatacgc agcaccggct caacagaatc
    17401 atgtccccca ccaccggcgg gcattcacac gcgagcccac cactcaagca ttcaccgtcg
    17461 acacgattcg cgcgcacatc gctttgtcag gcagcgggcc ggaccccgct cgaacgttgt
    17521 cagccatgta ttgcggacgc ccggttcctg ggatcaccac ggtcaccgcc ggttgcgcga
    17581 gtacgaattt gagcaggact tgcgcccagc tcgtgcagcc gatttcagca gcccaaccag
    17641 gtagtgcttg cttgccgaca cgcgcaagca gaccgccacc accgaaaggt tggttgacca
    17701 cgacaccaat gcccaactcc gccgcaagcg gcagaatgcg ctgctccgcg tcacgatcgt
    17761 ccgcggcgta gttgatctgc acgaagtcgg gtttttcact gcgcatcacg gctgcgacct
    17821 cgccgaacgc actggacgtg taatgcgtaa tgccgatata gcgaatccgg ccgcgtgctt
    17881 tccagtcgcg cagtgtggcg agctgggtgc gccagtcgag caggttgtga acctgcatca
    17941 gatcgatacg cggttgctga aagcggcgca gcgattcttc catctgcgct atgcccgccg
    18001 cacgcccttc ggtccagact ttagtggcaa cgaaggcttt cttgtgcgcg tcgagtcgtg
    18061 taagcaactc gccggcgacg gcttccgatg agccatacat cggcgacgaa tcgatcgtcg
    18121 aaccgcctgc ttcgaacaga atccgcaaca cgtcggcaag ttgcgactgc gcggcggcgt
    18181 cattgccgac atcgaaggtg cgccacgtgc cgcagccgac cacgggcagg cgttcgcctg
    18241 tcgagggaat cacgcgttgc gccatgcgtg gcagcggcgt cgtggtggtt tcagtttgtg
    18301 tttgtgtttg ggattggcct tgagccggcg tgatatgcgg caaagcaaac gagacaggca
    18361 agccaattgc ggcgctgccc gctgtgcgca gaaagcggcg tctgccgtga cgccgggcat
    18421 cgtggttcga cggaattgcc atagcgtgcc ctcgatatgc aaggagtagt gtgactctgc
    18481 acgatacggc aattcggcgc tgtgcgtgat ggcgccgggc atgcccttct caagagccga
    18541 cggctcgctc gatgactcgc tatgagttga attcgatcca atgcaccggt cagtttgctg
    18601 cgttttccag ttccggcgtc gggcccccgc cagcgcgcca tcagttcggg cttggtccac
    18661 gctgcgtaga cccgggccgg cgatgccttg atgcgccgga caatcgtcaa gctcggcttt
    18721 tccatggtca ttccacttgt ccctccacga aatcggcaag ccggtcgacg cttgcggacc
    18781 agaagcgctc gtagtgcctc aaccatgcca tcgcctcctc cattggaccg gcgatcagat
    18841 cgaccgacac ggtccgcccg ttcttcttgc gcgcgaccag acccgcgtcg ctcagcacgt
    18901 cgaggtgctt catgaccgcg gggagcttga gcggcagcgg acccgcgatt tccgtgatcg
    18961 acaggccggg ctcccgctcg aggcgagcga ggatggcccg gcgtgtcggg tccacaaggg
    19021 cggaaaacgt gcgatcgagc tgactgactg gagactttgc catatggtga actatatgcg
    19081 gtcagtgacc ggtgccaaga tttttcatga acggcacggc ttgcccggcg tcctgtttgc
    19141 ggccggttgc cagtccgttc tagactgcgg gtcggtgaca atcgagagag caaccccgta
    19201 tggacattct tcaaggcatg gcggtattca agcgtgtcgt cgatgcatcg agcttctcga
    19261 aggcggccga ttcgctggag atgccgcgtg cgtcggtatc gaccctcgtg cagaatctgg
    19321 agagccatct gggggtacgc ctgctgaatc gcaccacgcg cagcgtgcgg gtgaccgacg
    19381 acggcgcgct ctactacgaa tactgcgcac gcattctcgc cgaggtttcc gacgccgagt
    19441 ccgcgttatc gaacaagctg caaaatccgc gcggcacgat tcgggtcgac acctccgcca
    19501 catttgccac caacgtgctg ctgcccatga tgcgtgattt caatgcgcgc taccccgaca
    19561 tcaccgtgaa gctggggctg gccgaccgca acgtcgatct cgtgcaggaa ggcgtcgatt
    19621 gcgtgatccg gatcggcacg ctcgacgatt ccagtctcgt cgcacggccg atcggcagcg
    19681 tacgcatgtg catctgcgcc gcgccggcct atctcgagca gatgggcgag cccgccacgc
    19741 ccgatgatct cgaccgccat cgtgccgtca attatttttc cgcgcgcaca gggcgtgtct
    19801 atccattcga gttcaaagtg gacggcacgc tgattcgaaa gccggtggac ggcatactcg
    19861 gggtcaacga cggcctcgtg tacgtgatgt ccgcgatcga aggactgggg ttgatccaga
    19921 cgccacgctt catggctgcg caggcgcttc gcactggcgc gttacgtgaa attctccgcg
    19981 actatccgtg tccgcctatg ccgctttcct tgctgtatcc gcagcgtcgg ctgagcatgt
    20041 gcgtgcgggt gttcagcgag tgggttcacg agctcgcgct gaacaatccc gatctcaatg
    20101 gcgaggcgga cgcttaacca ctccctgtat cacggatttt cccgccgtca tagacgtaag
    20161 gccgccggcg cctgctcgcc ggcaatcaga cgccgttgcc gccgactgtc ttgatgctct
    20221 tccattgtcc ttgctggacc tgaaacagcg tgtacggcgg cttgatcaga tcgccatagg
    20281 gatcgaaggt gatatcgccg gtcacacctg tcaccttgac cttcgagagg ctgtcgacga
    20341 tcttcgcgcg atcgagcgag tcggcgccgt gaatcgcctt gatcatggca agcgcggcgt
    20401 cataggcgaa cggcgcatac agttcgacat cctgattgaa gcgtgcttta tagcgcttgc
    20461 cgaatgcctg cgcggcgggc agcttatcga gcgccggacc gggctccaga tcctgagtgc
    20521 cgtcacccgc cgtgcccgcg atctgcagga atgcattgct cttcagcgca cccgcgccga
    20581 acagttgcgc agtcatgccc agcgagcgca tctgcttaac cagcatcgca ccctgttcat
    20641 ccagaccgcc gaagaaaatc agatcgacgt tcttgctctt gagcgtcgtc aggatggcgc
    20701 ggaaatcgac cgctttgtca ttggtgaatt cgcggtcgac cacgttgccg ttcgcctctt
    20761 tcgccccctt ttcgaatgca tc
//
LOCUS       DATASH010000293         3764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3996273,
            whole genome shotgun sequence.
ACCESSION   DATASH010000293 DATASH010000000
VERSION     DATASH010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3996273"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..610
                     /locus_tag="VHV99_08245"
     CDS             92..610
                     /locus_tag="VHV99_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018423926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEX3635464.1"
                     /translation="MCAVLTFAAVSARAADAFALSSPGLADGGTLDSSHAASANNCGG
                     GNVSPALQWRNAPAGTKSYAITIFDPDGAKGLGIVHWVLYGVAPSATGLGAGATPPAG
                     SVGGTNRTGGSGYYGPCPPIGEVPHHFVVQIYALDLPPDALQAGLTRDALQAAIKDHV
                     LAATSTVLRYGR"
     gene            complement(828..1823)
                     /locus_tag="VHV99_08250"
     CDS             complement(828..1823)
                     /locus_tag="VHV99_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018438319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX3635465.1"
                     /translation="MQYRKFGHTGLTVSRLCLGTMTFGLQTEEDVSQRILDTAADAGV
                     NFIDTADVYPLGGSEDVAGRTEEIVGRWLKGKRDRFVLATKAVGKMGPSPWDQGASRK
                     HLLDAIDASLRRLGTDYVDLYQLHSDDADTPLDETLEALDVIVRSGKARYIGVSNFLA
                     YRLARALGRADVLRVARFVSVQPRYNLLFRQIERELLPLAAEEQLAVMPYNPLAGGLL
                     TGKHRVDAAPTSGRFSETVGKAGAMYQQRYWHQREFETIEKLKGIAASTGESLTRVSL
                     AWVLANPLITSAIIGASRAEQLSDTLAASEFVLDAEVKSQLDEASHEYRWGDAAR"
     gene            2415..>3764
                     /locus_tag="VHV99_08255"
     CDS             2415..>3764
                     /locus_tag="VHV99_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006417007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX3635466.1"
                     /translation="MFNHTPLVAALALAFAVPFATPAVAQTAPQPSAENAPTTAAGDN
                     ATLPTIGVAAQAEQQDFQAERATVGAKTPTALRDIPQTVTVINKAVLASQGATSFQDA
                     LRNAPGVTIGAAEGGQIGNNVNLRGFTAQNDIYLDGFRDRNQYYRDTFDLEELEVLYG
                     PSSMLFGRGSTGGVINQVSKKANLKDSAEVSGMIGTDDKYRSTVDVNHKLTDTSAIRL
                     NAFGQSLGSTRDEMKNKDYGIAPEVRFGIGTPTEVTISALIQHNYDMPDYGVQALNGR
                     PSPVPKNTFYGLTTDRTIQDVQTFTAGVKHKFSDNLILTNQTQFSHSMTDARETAPQA
                     VLTGPLASSTALTNGNFTTLSPSQLFIKLQSHDRVIENHSLYNDTMLEYKFDTGPIKH
                     DLITGIDLGHDSYSNQASTRNNLPVVSMVNPPYLSSPAGVTTAVGNYTDSGSNEIGAY
                     "
BASE COUNT          792 a         1176 c         1083 g          713 t
ORIGIN      
        1 tccttttcaa gtcggctaac ttatacccgg gcaatcaaca atgattcgaa gcgataacgc
       61 tggcgttgca cgacgccttc tatctgtcgg tatgtgcgcc gtgctgacct ttgccgccgt
      121 gagcgcccgc gcggctgacg cgttcgcgct gtcgtcgccc ggtttggccg acggtgggac
      181 gctggactcg agccatgcgg cgagcgcgaa caattgcggt ggtgggaatg tgtcgcccgc
      241 gctgcaatgg cggaatgcgc ctgccggcac gaagagctac gcgatcacga ttttcgatcc
      301 ggacggcgcg aagggattgg ggattgtgca ctgggtgctg tatggcgtcg cgccctcggc
      361 gaccgggttg ggtgctggcg ctacgccgcc ggccggcagt gtgggcggaa cgaatcggac
      421 gggcggctcg ggttattacg gaccgtgccc gccgatcggc gaggtgccgc atcactttgt
      481 cgtgcagatt tatgcccttg atttgccgcc cgacgcactg caggcaggtc tgacgcggga
      541 cgctttacag gcggctatca aggatcatgt gctggctgct accagtactg tgcttcggta
      601 cgggcggtga ttttttgcct ttggtcttgt ctgcggggcg gccgggcaac tggcggcgcc
      661 ggtaagggca acttccgcca gcaaaggcaa cctcggctgg caaaggcaac ctcagctggc
      721 aaaggcaacc tctgccggca aaggcaacca ctgccggcaa aggcaacctc cgctggcgaa
      781 ggcaacctcc gctggcgaag gcaacctctg ccggcaaagc caccccatta cctggccgca
      841 tcaccccatc gatattcgtg gctcgcctcg tcaagctgcg acttaacctc tgcatcgagc
      901 acaaactccg aagccgccag cgtatcgctc aactgctcag cgcgactggc gccaataatt
      961 gccgaggtaa tcagcggatt agccaacacc caggcaagcg agacccgagt caacgactcg
     1021 cccgtcgacg cagcgatccc cttcaacttc tcgatcgttt cgaactcgcg ctgatgccag
     1081 taccgttgct ggtacatcgc gccggccttc ccgacggttt cgctaaagcg cccggacgtg
     1141 ggcgccgcat ctacccgatg cttacccgtc agcaagccac ccgccagcgg gttgtaaggc
     1201 atgacggcta attgctcttc agccgccaaa ggcaacaatt ccctttcgat ctgccggaac
     1261 agcaggttat accggggttg caccgagacg aagcgcgcga cgcgcaacac atccgcgcgc
     1321 cccagcgccc gtgccaggcg ataggcgagg aaattcgata cgccgatata gcgcgccttg
     1381 ccggatcgca cgatcacgtc cagcgcctcc aacgtttcat caagcggcgt atccgcgtcg
     1441 tcggaatgga gctggtaaag gtccacgtag tccgtgccaa gccgccgcag cgatgcgtcg
     1501 atggcatcca gcagatgctt gcgcgatgcg ccttgatccc acggtgacgg ccccatcttg
     1561 ccgaccgcct tggtcgccag aacgaagcgg tcgcgtttgc ccttcagcca gcggccgacg
     1621 atttcctccg tgcggccggc cacatcctcg ctgccgccaa gcggataaac gtccgcggtg
     1681 tcgataaagt tcacgccggc gtccgccgcg gtgtcgagaa tccgctgcga aacatcttct
     1741 tcagtctgca ggccaaaggt cattgtgccg aggcacaggc gcgaaacggt caggcctgtg
     1801 tggccgaatt tgcggtattg catagttatc tccgaagcag gacggaacag ttgcggtgtg
     1861 cagtgttgca aaaaggataa acggtgtcgc gattttacgt ttggccggtg gcgcgtgcag
     1921 gcgcagttat ccgtaagcat ttagaaagat taacgcgatg ctcaacttac atgagcaagt
     1981 gcagcgcgtt tccatcgtcg gagcacatca tccggataac gtcgcagatt ctgcggtgag
     2041 ttcgcaggag ttggcgcggt tatgtcagat ataggcaggt tcgcctattc atctgattaa
     2101 cattcagagt cctttcatgt cctgtcgccg gacattacaa atacgtaata ttcccccggc
     2161 gcccacctat aaaaaattac aaaacccata cgtttgtaaa aaaaagaaac aattcaatta
     2221 caccaggtaa tgttcagcgc gattgatgca tttttatgca tgtggaatga tagcgattct
     2281 catttaacca actagccagc catcccgtcg tctttgccct aagtagcaga ccggcagcca
     2341 gccaattgcg gttttgtcat tgatccctgc cgagtcatgc ggatctggca agcgcacttc
     2401 tgacgggatt tcccatgttc aatcacacgc cgctggtggc tgcgttagcg ctagcttttg
     2461 ccgttccatt tgccacgccc gccgtcgcgc agacggctcc gcagccttcg gctgaaaacg
     2521 cgccgacaac cgcagcaggc gacaacgcaa ccttgccgac catcggcgtc gcagcgcaag
     2581 ccgaacaaca ggacttccag gcagaacgtg cgacggtggg tgcaaagacg cccaccgcgt
     2641 tgcgcgatat tccgcagacc gtcaccgtca tcaacaaagc ggtgctggcc tcacagggtg
     2701 cgacgtcgtt ccaggacgcg ctgcgcaatg cgccgggcgt gacgatcggc gcggccgagg
     2761 gcggacagat cggcaacaac gtcaacttgc gcggctttac cgcacagaac gacatctatc
     2821 tcgacggctt ccgcgatcgc aaccagtact atcgcgacac cttcgacctc gaggaactcg
     2881 aggtgctgta cggcccgtcg tcgatgttgt tcggccgcgg ctcgaccggt ggcgtgatca
     2941 accaggtcag caagaaagcg aacctgaagg actcggcgga agtctcgggc atgatcggca
     3001 ccgacgataa atatcgcagc accgtcgacg tcaaccataa gctgaccgac acgtccgcga
     3061 tccgcctgaa cgcattcggc caaagcctcg gctcgacgcg cgacgagatg aagaataagg
     3121 actacggcat cgcgccggaa gtgcgcttcg gcatcggtac gccgactgaa gtcacgatct
     3181 ccgcgctgat ccagcacaac tacgacatgc ccgactacgg cgtgcaggcg ctgaacggtc
     3241 gtccgtcgcc ggtgccgaag aacaccttct acggcctgac caccgaccgc acgattcagg
     3301 acgtccagac tttcactgcg ggggtcaagc acaagttctc ggataacctg atcctgacga
     3361 accagacgca gttctcgcac tcgatgaccg acgcgcgcga aaccgcgccg caagccgtct
     3421 tgacgggccc gctcgccagc agcaccgcgc tgacgaacgg caatttcacg acgctatcgc
     3481 cgtcgcaact gttcatcaag ctgcaaagcc acgaccgtgt catcgagaac cactcgctgt
     3541 acaacgacac gatgctcgaa tacaagttcg acaccggtcc gatcaagcac gacctgatca
     3601 ccggtatcga cctcgggcac gacagctatt cgaaccaggc atccacgcgg aacaacctgc
     3661 cggtcgtttc gatggtgaat ccgccgtatc tgtcgtcgcc cgcgggcgtg acgacggcgg
     3721 tcggcaacta cacggactcg ggttcgaatg agattggcgc gtac
//
LOCUS       DATASH010000294         1940 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3206044,
            whole genome shotgun sequence.
ACCESSION   DATASH010000294 DATASH010000000
VERSION     DATASH010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3206044"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VHV99_08260"
     CDS             <1..252
                     /locus_tag="VHV99_08260"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579561.1"
                     /note="catalyzes the oxidation of malate to oxaloacetate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEX3635467.1"
                     /translation="NAAIDHVRDWVLGTNGKWVTMGIPSDGSYGIPEDIIYGVPVTCE
                     NGEYKRVEGLEIDAFSREKMDGTLNELLEEREGVQHLLG"
     gene            560..1576
                     /locus_tag="VHV99_08265"
     CDS             560..1576
                     /locus_tag="VHV99_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEX3635468.1"
                     /translation="MRALTPAEVLFDGEVPPAVLPACDHYAGSEKLMLKSLALQQQLG
                     PVFDITLDCEDGAQVGREAEHAELVASLLGSEQDHFGRVGVRIHDFDHPHWRDDVRLI
                     LRAAKGAPAYITLPKIRNVPDAAEMVAFIEATRSELGIAQPVPVQLLVETHGALTRVF
                     DLAALPGVEALSFGLMDFVSAHDGAIPDTAMRSPGQFDHPLVRRAKLEISAACHAHGK
                     VPSHNVSTEVRDMSVVANDATRARNEFGYTRMWSIHPAQIEAIVAAFAPRDEEIVTAT
                     EILLAAQSAQWGPTRYHDTLHDRASYRYYWSVLRRAQSTGRIVPQDAAPLFTKVGANG
                     QTAS"
     gene            1737..>1940
                     /locus_tag="VHV99_08270"
     CDS             1737..>1940
                     /locus_tag="VHV99_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEX3635469.1"
                     /translation="MSETTQTAAAQGENEPNSGFRPKKSVALSGVTAGNTALCTVGKT
                     GNDLHYRGYDILDIATSCEFEEIA"
BASE COUNT          397 a          615 c          598 g          330 t
ORIGIN      
        1 aacgctgcaa tcgaccacgt gcgtgactgg gtgctcggca cgaacggcaa gtgggtcacc
       61 atgggtatcc cgtcggacgg ttcgtatggc attccggaag acatcatcta cggtgtgccg
      121 gttacatgcg aaaacggcga gtacaagcgc gttgaaggtc tggaaatcga cgcgttctcg
      181 cgcgaaaaga tggacggcac gctgaacgag ctgctggaag agcgcgaagg cgttcaacat
      241 ctgctcggct aagcgcctga tgcagacgaa ccggaactcg tgagaaaacg ccgggccgcc
      301 ccacgttttc tcaccccctc ggggggcctg gcgcgaagcg acaggtctgg gtgcactccc
      361 cgactttatc gtggcgtgtt ccggtttttt acgtgcgaat gcaggtacga cgctgcaatc
      421 tgcggctggt ttcgcagacc accgtgccag tattcgcatc tgattcgaac gcgttttgtg
      481 cagttgcctg acagtggccg tacagagcgc atccgcatca gattttctct aaacacaacc
      541 cacgctcctg acgccgaaga tgcgcgccct cacacccgcc gaagtgctgt ttgacggcga
      601 agtgccgccc gctgtgctgc ctgcctgcga tcactacgcc ggcagcgaga agctgatgct
      661 gaaatcgttg gcattgcagc agcaactcgg cccggtgttc gacatcacgc tcgattgtga
      721 agacggcgcg caggtcggcc gggaagccga acacgcggag ctggtcgcgt cgctgctggg
      781 cagcgagcag gaccatttcg gccgcgtcgg cgtgcggatc cacgacttcg atcatccgca
      841 ctggcgcgac gacgtccgcc tcattctgcg tgccgcaaaa ggagcgcccg cctacatcac
      901 cctgccgaag atccgcaacg tgcccgacgc cgccgaaatg gtcgcgttta tcgaagcgac
      961 gcggagcgaa ctcggcattg cgcagccggt gccggtgcaa ttgctcgtcg aaacccatgg
     1021 cgcgttgacg cgtgtgttcg atctggccgc gctgccgggc gtcgaagcgc tgagcttcgg
     1081 gttgatggac ttcgtctccg cgcacgacgg cgcgattccc gataccgcga tgcgctcgcc
     1141 cggccagttc gatcatccgc tggttcggcg tgccaagctc gagatctcgg cggcctgcca
     1201 tgcgcacggc aaagtgccgt cgcataacgt cagcacggaa gtacgcgaca tgagcgtagt
     1261 cgcgaacgac gctactcgcg cccgcaacga gttcggctac acgcgcatgt ggagcatcca
     1321 cccggcgcag atcgaggcga tcgtcgccgc gtttgccccg cgtgacgagg aaatcgtcac
     1381 cgcgaccgaa attctgctgg ctgcccaatc cgcgcaatgg ggaccgacgc gctatcacga
     1441 cacgctgcac gaccgggcga gctatcgcta ctactggtcg gtactgcgtc gcgcgcaatc
     1501 cactggccgc atcgtcccgc aagacgccgc gccactcttc acgaaagtgg gcgctaacgg
     1561 gcaaaccgca tcatgaacat gacggccaac gctacagccg gcgcggcaac gctgaacagc
     1621 ggccacgcgc gccgccatgc aagcggatca cggcatcgtc tggctttgtg gaaaacaggc
     1681 gtcgaacacg cggttcaacc agaagcaacc agcaggcaga aaggagattg cgggagatga
     1741 gcgagactac gcaaaccgcc gcggcacaag gcgagaacga acccaacagc ggcttcaggc
     1801 cgaagaaatc cgtggccctg tccggcgtga cggctggcaa cactgctttg tgcacggtcg
     1861 gcaagaccgg caacgatctg cattaccgcg gctacgacat tctcgatatc gcgacctcat
     1921 gcgaattcga agagatcgcg
//
LOCUS       DATASH010000295         9436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6963461,
            whole genome shotgun sequence.
ACCESSION   DATASH010000295 DATASH010000000
VERSION     DATASH010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 9436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9436
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6963461"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VHV99_08275"
     CDS             <1..208
                     /locus_tag="VHV99_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635470.1"
                     /translation="AGFAHSQNMPEPQQPTSQENAATQPAASPSDSGTQAYGGTPDTK
                     MQSGWKHMKPCSTDPQCNIFFGGS"
     gene            934..1155
                     /locus_tag="VHV99_08280"
     CDS             934..1155
                     /locus_tag="VHV99_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635471.1"
                     /translation="MQVDVAVHAASRSNGFGLPHLRELKPAFPSSYSVTLNGSVHLAA
                     TVCVCAHVPIDFAADTSAVEETSGELELF"
     gene            1186..1410
                     /locus_tag="VHV99_08285"
                     /pseudo
     CDS             1186..1410
                     /locus_tag="VHV99_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006495102.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
     gene            1323..1628
                     /locus_tag="VHV99_08290"
     CDS             1323..1628
                     /locus_tag="VHV99_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012402899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3635472.1"
                     /translation="MFHVLADGPALEALGQAVSTFCVQPEIKLVISDVNMPNMDGLTM
                     VEKIRGELANTSVNVIMLTTESSPAMKERGKAAGVKGWIVKPFKGEAVLETFRKLAS"
     gene            complement(1981..2946)
                     /locus_tag="VHV99_08295"
     CDS             complement(1981..2946)
                     /locus_tag="VHV99_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011494020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3635473.1"
                     /translation="MTTTENDAIDRFFRSGLKLPHLRILVSLAELGQVTRVAAAFHVT
                     QPAISKQIGEIEEALGVPVARRVGNAVELTSIGKVMAVRGREILRHIELARRDVSALT
                     TGTAGHVRLGAVATIPQPLIAHAVELFMRRAPWATLSFAEATLDKLIKMLDEGELDLA
                     LGRNRITGNAGVLSQQTLLREPFVFVVGARHPLGKVEMPVSWNDLRSARWITPLRGSP
                     AFATLAEALAEHGIPLDRGAVESSSLTLNLSLLIGGDFVSILPLSVARHHVSRGRMRV
                     LPLAPLEPLGEVVLVWRADTAAPAADLFSECLRESSSELLEGEAE"
     gene            3185..4522
                     /locus_tag="VHV99_08300"
     CDS             3185..4522
                     /locus_tag="VHV99_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011494021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3635474.1"
                     /translation="MSTPLYGGAERPAVPDEPSEPAESAERAALYRKLAWRIVPFLFL
                     AFIVAYIDRVNVSFAKLQMLTDLSLSETVYGAGAGIFFLGYFIFEVPSNLILHRVGAR
                     IWIARIMVTWSIVSCLTMFASGATSFYVLRFLLGVAEAGFFPGIVLYLSTWFPSNRRS
                     QIIALFMAAIPVSGAIGGPVSGWIMQQLAGVHGLAGWKWLFLIEGIASLVVGVAAFFV
                     LHDRIESVSWLNREEKALLARDLEADAQTRAHQTVRGAFTNPKVWLLGLLYFCIAMGN
                     YGLVFWLPTMIRASGVVSVANIGLLSAVPSLISAIAMILIARHADKHDERRKHVAVCA
                     VLGGAGMLASVLLGQHVWLSMAALVVAAIGINSIAPVFWGIPTALMGGVGAAAAIAVI
                     NCTGNLAGFVSPYVIGFLKDTTGTLLPGMIVLACALVLAAVIVMAMPATRKTA"
     gene            4533..5312
                     /locus_tag="VHV99_08305"
     CDS             4533..5312
                     /locus_tag="VHV99_08305"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3635475.1"
                     /translation="MTQQVCMVTGAATGIGAATALRFASAGWAVAIGNFDDSTRDAAL
                     AVEAQCRQLGAETLVFDADVGSDAACVKAVDAVASKWQRIDALVNCAGTTRFIPHGDL
                     AAIDDLEFERVYRVNLIGMFQMTRAAVPVLRDSSTASHSAAVINISSLASLNGTGSSI
                     AYAASKGAVNTLTLSLARNLAPHIRVNALAPGMVDDGLPRRVLGDEAYAKIVDSMTKV
                     APLKRVSQPGEIAELAWFIAAHAPSMTGQVIAAENGMRLNG"
     gene            5362..7089
                     /gene="ilvD"
                     /locus_tag="VHV99_08310"
     CDS             5362..7089
                     /gene="ilvD"
                     /locus_tag="VHV99_08310"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX3635476.1"
                     /translation="MSNLHKHRSRTVTEGITRTPHRAFLRATGMDDAAIEKPFVAIVD
                     TFGENTPCSMSLAAVSDNVRLGVAAGGGVPIRGSAISVSDGTSMNHSGMRFSLVSREI
                     IADSVEVFVRAHCYDAFVGVAGCDKTLPGILMGMVRVNVPGVFLFGGAMLPGIAPDGT
                     QATILTAIEAVGAAQRGDMSLETLRGIEKRCTPGAGSCPGQFTANTMAMVAETLGLAP
                     LGSAMVPAVYSERIAIARRAGETVMNTLKSGGPLPRDLVTRKSLENACAAVAATGGST
                     NAALHIPAIAHEAGIRFTLDDVSEVLARTPLIGDLQPGGKYLALDLYHVGGVPAVLNA
                     LLAGGFLHGDTLTQTGETLETALKAFAGPDGRVVRPHNEPLSENAGLVVLRGNLAPDG
                     ACLKTAGLKSLVFSGTARVFETEEACMSVVSKRTYQEGDVLVIRNEGPKGGPGMREML
                     SVTAAIYGQGMGEKVALLTDGRFSGATRGMCIGYVGPEAAAGGPIRLLRDGDRIHIDA
                     HKGTLDVALSDEELARRSKEAVPFTRGRLGGVLEKYEALVRPANLGAVTHSGAVEWPY
                     EASIGEEEA"
     gene            complement(7505..8263)
                     /locus_tag="VHV99_08315"
     CDS             complement(7505..8263)
                     /locus_tag="VHV99_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3635477.1"
                     /translation="MTSSSPIRAIVTGHTRGLGASLAEQLLARGVAVLGLSRSRHATL
                     KDRFPALLEEIELELADPARVAQWVATDALRGFVRGARSVLLINNAGTVQPIGPMEGQ
                     NATDIATAVSLNVATPLMLAAAVTTASVDATDRRIVHISSGAGRNAYPGWSIYCATKA
                     ALDHHARAVALDANRALRICSLAPGVVDTNMQAEIRGSGAEQFPLREKFEDLKRNGQL
                     ATPEQCATQLLDYAFSDAFGQTPVADIREVAKQG"
     gene            complement(8427..9389)
                     /locus_tag="VHV99_08320"
     CDS             complement(8427..9389)
                     /locus_tag="VHV99_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HEX3635478.1"
                     /translation="MPTVAIAIFPGVQALDVAGPVDVFSEANRFVAPQERYEVALVSA
                     EPGPLRASNGMALIADATFEQMRRPFNLALVAGGPALPDGKASDQRLLDWLSDTATQC
                     ERYGSICTGAFALGHAGLLDARNVTTHWQHAAQLAAQFPQARVDFDRIYLRDGTLVTS
                     AGVTAGIDLSLALVAEDHGPHTALAVAKRLVVFAQRQGGQSQFSPYLTAPADETSPVA
                     KVQAHVMERISESFTVKQLADVAGMSARNFARVFVQETNVTPHEFVERARVDAARKLL
                     ESSGAALKAIAYDCGFGTADRMRIVFSRRIGATPMQYRERFRSN"
BASE COUNT         1752 a         2839 c         3084 g         1761 t
ORIGIN      
        1 ggccggcttc gcgcattcgc aaaacatgcc ggagcctcag caacccactt cacaggaaaa
       61 cgcagcgact cagcctgccg cttcgccgtc cgacagcggg acgcaggcat acggcggcac
      121 gccggacacg aaaatgcaga gcggttggaa acatatgaag ccctgcagta ccgatccgca
      181 atgcaatatc ttcttcggtg ggagttaaaa acaaactgat gtcgcgccgg acaataacgg
      241 caaacaaaac gggcgtgggc cacaaagcgt gacaaacgtt agccgtcgtg cgcgaccatc
      301 cagtccaggt aggtaatttc cagaatggtg agtgattcca ggagacggcg ctatctcgct
      361 caacggtgtg gaattccagg agacggcgcc acctcgctca acggtgagga attccaggag
      421 atggcgctat ctcgcccaac ggtgaggaat tccaggagat ggcgccacct cgctcaacgg
      481 tgaggaattc caggagatga catcaccgcg gccaaaggtg agcgattcca ggagatgtcg
      541 ttcaatcgat taaaagtgcg ctgcgccaat ggcagcgcat caccctgcca gaagatgtta
      601 ttccagccgc cgagcagcac cgtggaagca cgctaagaca tccaaggtga ggcgttccag
      661 gcgatcactc gcaaaaggtg agtgattcca ggagcggaat aattgccgtg ttgaacccgc
      721 acctgaaata cggtgaggat ttccaggaaa tcctcctcat ggcagccgac tgcgaagcag
      781 ccctcagacc gccttcaaac aagcgcgcaa aaagccatag gtgagaacat tcaggagctg
      841 acgagatgaa aataacgcta cttgtccgca cactttgaac tctaaggtga gggaattcgg
      901 gagcgtgcgc ccggggccgg ctcggacgtg ctcttgcaag tcgatgtagc cgtgcacgcg
      961 gcctcgcgtt cgaacggatt cggcctgccg catctgcggg aattgaagcc ggcgtttcca
     1021 tcgtcgtatt cggtcacgct gaacggctcg gtgcacctcg ccgcgacggt ttgcgtctgt
     1081 gcacatgtgc cgatcgactt cgcggcggac acgagtgccg ttgaagaaac cagcggtgag
     1141 ctcgagttgt tttgatcatc gagtcgatat ctgcaatgag cggggacgaa tctgatttcg
     1201 ggttcgttca agaaccgcaa tgtcggcgat gcggatgcat ggctgcgcag tcaggcgcaa
     1261 gtgccgctgc tggggaatca caggcataag tgcatctcgt tcggcagcgg caatccacaa
     1321 ctgtgtttca tgtactcgct gacggacccg cactcgaggc gctcggtcaa gctgtatcaa
     1381 ccttttgtgt tcagcctgag atcaagctgg tgatcagcga cgtcaacatg ccgaacatgg
     1441 atggcctgac gatggtcgag aaaatccgcg gtgaactcgc gaacacgagc gtcaacgtga
     1501 tcatgctgac gaccgagagc agcccggcga tgaaagagcg tggcaaggca gccggcgtga
     1561 aaggctggat cgtcaagccg ttcaagggcg aagcggtgct ggaaacgttc cgtaagctgg
     1621 cgagctaaac gcggcaagcg cggcggccgc gcgcaaaggt gaggtttttc gggagcacgc
     1681 gcgcgggtga tttgcaaagc atgattcgct atagccgaag tcctcgcgac ctcgaaatac
     1741 gcgccctccg aattgtcccc cgttccggcg tgatatcgct tcaacctacg taagcgtgtc
     1801 gccctggaag cgatcgcgcg attgtcgctt tcatattcat cgaagaatga gttggtttgc
     1861 cgacgcaaag cagccggcct tccgaaggct ttccaaaggt cttccaagtg ccttcaaaaa
     1921 gcgcaaaggt gagaacattc gggagtcggc ccacaatcgc gatacgtgag ccgcgcccca
     1981 tcactccgcc tcaccctcca gcaattccga cgacgactcc cgcaagcatt cgctgaacag
     2041 atccgccgcc ggcgcagcgg tatccgcgcg ccacaccagc acgacttcac ccagcggttc
     2101 gagcggcgcg agcggcaata cgcgcatgcg tccacggctc acatgatgtc gcgcgaccga
     2161 gagcggcagg atcgacacga agtcgccacc gatcagcagc gacagattca acgtcaacga
     2221 actcgattca accgcgccgc gatcgagggg aatgccgtgc tccgctaacg cctccgccag
     2281 cgtcgcgaaa gcgggtgaac cgcgcagcgg cgtaatccag cgcgccgagc gcagatcgtt
     2341 ccagctcacc ggcatctcca ccttgccgag tggatgtctc gcacccacga cgaacacgaa
     2401 cggctcgcga agcaaggtct gctggctcaa cacaccagcg ttgccggtga tccggttacg
     2461 gccgagcgcg agatcgagct cgccttcatc gagcatcttg atcagcttgt cgagcgttgc
     2521 ttccgcgaac gacagcgtcg cccaaggcgc gcgccgcatg aacagttcga ccgcatgcgc
     2581 gatcagcggc tgcggaatcg tggcgaccgc gccgaggcgc acgtggccgg ccgtgccggt
     2641 ggtcagcgca ctgacgtcgc ggcgcgcgag ttcaatgtgc cgcaggattt cgcggccacg
     2701 cacggccatc actttaccga tgctggtcaa ctcgaccgca ttgccgaccc tgcgcgcgac
     2761 cggcacgcca agcgcctcct cgatttcgcc gatctgtttc gaaatggccg gctgtgtcac
     2821 gtggaaggcg gccgcgaccc gcgtcacctg tccaagctcc gcaaggctca ccaggatgcg
     2881 cagatgcggc agcttcaggc cgctgcgaaa gaagcggtcg attgcgtcgt tttcggtggt
     2941 tgtcatcacg gtttattcgc tgcacataac tgaacggtaa tgccctgcgc cgaaacggtg
     3001 atttgccgtg cggcgcttaa tccggctaat cttcaatatc ggcaggacgg tagagcgtga
     3061 agcgggtggc tgaggatagc aggtgcgctt cgaccgcatg actcagggta taaccggagc
     3121 cgctccatgc gccgtatcgc cgcgaccaca gcgcgtattc aaaagcgcgc ggaaggagac
     3181 atgcatgagc acccctctat acggcggcgc cgagcggccc gctgtgcctg atgaaccgtc
     3241 ggaaccggcg gaatccgctg aacgcgcggc gctgtaccgc aagctcgcct ggcgcatcgt
     3301 gccgtttctg tttctcgcct tcatcgtcgc gtatatcgat cgcgtgaatg tgagcttcgc
     3361 caagttgcag atgctcaccg atctctcgtt gagcgagacc gtgtacggcg cgggcgccgg
     3421 catcttcttt ctcggctatt tcatcttcga ggtgccgagc aacctcatct tgcatcgcgt
     3481 cggcgcgcgc atctggatcg cgcggatcat ggtcacgtgg tcgatcgtct cgtgtctgac
     3541 gatgtttgcg agcggcgcga cctcgttcta cgtgctgcgc tttctgctcg gcgtagccga
     3601 agcgggcttc tttccaggca tcgtgctgta tctgtcgacg tggtttccgt cgaaccggcg
     3661 ctcacaaatc attgcgctgt tcatggcggc gattccggtg tccggcgcga tcggcggtcc
     3721 ggtgtcgggt tggatcatgc agcaactcgc aggcgtgcat ggcctcgccg gctggaagtg
     3781 gctgttcctg atcgagggga tcgcttcatt ggtggtgggc gttgcggcgt tctttgtctt
     3841 gcacgatcgc atcgaatcgg tgagctggct gaacagagag gaaaaagcgc tgctcgcacg
     3901 tgacctcgaa gccgatgcgc agactcgcgc gcatcaaacg gtacgcggtg cgttcaccaa
     3961 tccgaaggtg tggctgctcg gcctgctgta tttctgcatc gcgatgggca actacggact
     4021 cgtcttctgg ctgccgacca tgatccgcgc aagcggcgtg gtgagcgtgg cgaatatcgg
     4081 cctgttgtcg gcggtgccgt cgctgatcag tgcaatcgcg atgatcctga tcgcacgtca
     4141 cgccgacaag cacgacgaac ggcgcaaaca cgtggccgtg tgcgccgtgt tggggggcgc
     4201 cggcatgctg gcctcggtgc tgctcggtca acacgtgtgg ctttccatgg cggcgctggt
     4261 ggtcgcggcg atcggcatca attcgattgc gccggtgttc tggggcattc cgaccgcgct
     4321 gatgggcggc gtgggggcgg cggcggccat cgcggtgatc aactgcaccg gcaatctggc
     4381 gggcttcgtg agtccgtacg tgatcggctt cctgaaagat acgaccggca ccttgctgcc
     4441 cggcatgatc gtgctcgcct gcgcgttggt gctggccgcg gtgattgtca tggcaatgcc
     4501 ggctacgcgc aaaacagcat gaggagagca ggatgacgca gcaggtttgt atggtcaccg
     4561 gggcggccac gggtatcggc gcggccaccg cgttgcgctt tgcgagcgcc ggctgggcgg
     4621 tggcgatcgg caacttcgac gacagcacgc gtgacgcggc gctggctgtc gaagcgcaat
     4681 gccggcaact cggcgctgag acgctggtgt tcgatgccga tgtcggttcc gacgccgcgt
     4741 gtgtgaaagc ggtcgatgcg gtggcctcga agtggcagcg catcgacgcg ctcgtcaact
     4801 gcgccggcac gacgcggttc attccgcacg gcgacctggc cgccatcgac gatctggaat
     4861 tcgagcgcgt gtatcgcgtc aacctgatcg gcatgtttca gatgacgcgc gccgcggtgc
     4921 ctgtgctgcg cgattcgtcg accgcttcgc acagcgcggc ggtgatcaat atttcgtcgc
     4981 tggcttcgct gaacggcacg ggttcttcca ttgcttacgc ggcttcgaag ggcgcggtca
     5041 atacgctgac gctctcgctt gcgcgcaatc tcgccccgca tattcgggtg aatgcgctcg
     5101 cgcccggcat ggtggacgat ggcttgccgc gccgcgtgct cggcgacgaa gcgtacgcga
     5161 agatcgtcga cagcatgacg aaggtcgcgc cgctcaagcg cgtgtcgcag cctggtgaga
     5221 tcgccgagct cgcgtggttc attgctgcgc atgcgccatc gatgaccggt caagtgatcg
     5281 ccgccgagaa cggcatgcgg ctgaacggct gaacggctga acggctgaac gcatagcaga
     5341 cgcacacgta caggaaaaac catgagcaat ctccacaagc accgttcgcg caccgtcacc
     5401 gaagggatca cgcgcacgcc gcatcgcgcg ttcctgcggg cgaccggcat ggacgacgcc
     5461 gcgatcgaaa aacccttcgt cgcgattgtc gatacgttcg gcgaaaacac gccgtgttcg
     5521 atgtcgctcg cagccgtttc cgacaacgtg cgcctcggcg tcgcggcggg tggcggtgtg
     5581 ccgattcgcg gttcggcgat ctcggtgtcg gacggcacct cgatgaatca ttcgggcatg
     5641 cgctttagcc tcgtgtcgcg cgagatcatc gcggacagcg tcgaggtgtt cgtgcgcgcg
     5701 cactgctacg acgcgttcgt cggcgtggcg ggttgcgaca aaacgttgcc cggcattctg
     5761 atgggcatgg tgcgggtgaa cgtgccgggc gtgtttctgt tcggtggcgc gatgctgccg
     5821 ggcatcgcgc cggacggcac acaggcgacg atcctgaccg cgatcgaagc cgtcggcgcg
     5881 gcgcaacgag gcgacatgtc gctcgaaacc ctgcgcggca tcgagaagcg ctgcacgcca
     5941 ggcgcggggt cctgtcccgg gcaattcacc gcgaacacga tggcgatggt cgccgaaacg
     6001 ctcggtctcg cgccgctcgg ctcggcgatg gtgcccgccg tatacagcga gcgtattgcg
     6061 attgcccggc gtgccggcga aacggtgatg aacacgctga aaagcggcgg tccgctgccg
     6121 cgcgatctgg tcacacgcaa aagcctggag aatgcgtgcg ccgcggtggc cgcgaccggc
     6181 ggctcgacca acgccgcgct gcacattccg gcgatcgcgc atgaagcggg catccgcttt
     6241 acgttggacg atgtgtccga ggtgctcgcc cgcacgccgt tgattggcga tttgcagccg
     6301 ggcggcaagt atctcgcgct ggatctgtat cacgtcggcg gcgtgcccgc ggtgttgaac
     6361 gcgctgctcg ctggcggttt tctgcatggc gatacgctga cgcagaccgg cgaaacgctt
     6421 gaaaccgcct tgaaggcgtt tgccggtcct gatggacgcg tcgttcgtcc gcataacgag
     6481 ccgctcagcg agaacgccgg gctggtggtg ttgcgcggca atctcgcgcc ggacggtgcg
     6541 tgcctgaaga cggcgggatt gaagtccctg gtgttcagcg gaaccgcgcg cgtgttcgaa
     6601 accgaagagg cgtgcatgtc ggtcgtatcg aagcgcacct atcaggaagg cgacgtcctc
     6661 gtgatccgca acgaagggcc gaagggcggt cctggtatgc gcgagatgct cagcgtgacc
     6721 gcggcgatct acggtcaggg aatgggcgag aaagtcgcgt tgctgaccga tggccgtttc
     6781 tcgggcgcga cgcgcggcat gtgcatcggc tacgttggac cggaagcggc ggccggcggc
     6841 ccgattcgtt tgctaagaga tggcgaccgg attcatatcg acgcgcacaa aggcacgctc
     6901 gacgtcgcat tgagcgatga ggaactcgcg cggcgcagca aggaggcggt gccgttcacg
     6961 cgtggccgcc tgggcggcgt gctggagaag tacgaagcgc tggtgcggcc ggcgaatctc
     7021 ggcgcggtga cgcactcggg cgcggtggaa tggccgtatg aggcgtcgat cggtgaggag
     7081 gaggcatgag cggtatgcgc aagcggtaag cgcaagcgcc gctgacacgc ccggcccgca
     7141 ccacctctta cctgggtatg cagattactc agacctatcc aaaggtgagg gtttgcggga
     7201 gcagcctgtt ctggaccctc ggcgccacgc ccagcatgcc tttgcgacct gcccccaaac
     7261 tcggaaggtg agcgttttcg ggagtcaccc gatgtgcgct gcgcgcctgg ccccgaccgc
     7321 tccgcgctaa aggtgagact tttcgggacc aggcgcacgc atcgtacagc gactttgcgc
     7381 gccgtccagt actacgttcc gattgggacg gccgagcgcg acgcgatgac tgttgcgccc
     7441 ggcgtgtgat tctcggcgta tcagctgaaa ggtgaggttt tacgggagcg tagagcttgc
     7501 cgcgtcagcc ttgcttcgcg acctcgcgaa tatcggccac gggcgtttgc ccgaatgcat
     7561 cgctgaaggc gtagtcgagt agttgggtcg cgcattgttc gggcgtcgcc aactgaccgt
     7621 tgcgcttgag gtcttcgaat ttttcgcgca gcggaaactg ctcggcgccg ctgccgcgaa
     7681 tttccgcctg catgttcgta tcgaccacgc ccggcgcgag gctgcaaata cgcagcgcac
     7741 gattcgcgtc gagtgccacg gcgcgtgcgt gatgatcgag cgcggctttg gtcgcgcagt
     7801 agatgctcca gccgggatag gcattgcgcc ccgcgccgct ggaaatatgc acgatgcgcc
     7861 ggtcggttgc gtcgacgctg gctgtggtga cggcggccgc cagcatcagc ggcgtggcca
     7921 cgttcaggct cacggcggtt gcaatgtcag tggcattctg gccttccatc ggaccgatcg
     7981 gctgcaccgt gcccgcgttg ttgatcaaca gtacgctccg cgcgccgcgg acgaagccgc
     8041 gcagtgcatc ggttgcgacc cactgcgcga cgcgcgcagg gtcggcaagt tcgagttcga
     8101 tttcttcgag cagggcgggg aaacggtctt tcagcgtggc gtgacgcgag cgggacaaac
     8161 ccagcaccgc gacgccgcgc gccagcagtt gttcagcaag agatgcaccg aggccgcgag
     8221 tatggccggt gacgatcgcg cggatcggag aagaagaggt catgttgttt acttgggatc
     8281 attggcttga cggggccgct attttctcac gccatattta cggcttgcag cgaggcggtt
     8341 tttcgaaccg cattccggct gcctatcagc aatccgcgag ccacattcat gccgtcctag
     8401 cccttcaagt cctcgccacg ccacattcaa ttagagcgga agcgttcgcg atactgcatc
     8461 ggtgtcgcgc caatccgtct cgagaagaca atgcgcatcc ggtccgcggt gccgaagccg
     8521 cagtcgtaag caatcgcctt gagcgccgcg ccactgcttt cgagcaactt gcgggccgca
     8581 tccacgcgag cgcgctcgac gaattcgtgc ggcgtcacgt tcgtctcctg cacgaacacg
     8641 cgcgcgaaat tccgtgcgct cattcccgcg acgtcggcga gttgcttcac cgtaaagctc
     8701 tcgctaatcc gttccatcac gtgcgcctga actttggcga ccggcgaggt ttcgtcggct
     8761 ggtgccgtga gataggggct gaactgcgac tggccgcctt gtcgctgggc gaacacgacg
     8821 aggcgcttgg cgacggccag cgccgtgtgg ggtccgtgat cttccgctac gagcgcaagc
     8881 gacagatcga tgcccgccgt gacgcccgct gaagtcacga gcgtgccgtc gcgcagatag
     8941 atgcggtcga aatcgacgcg tgcttgcgga aattgcgcgg cgagttgtgc cgcgtgctgc
     9001 caatgggtcg tgacattgcg cgcgtcgagg agtccggcgt ggccgagcgc gaaagcgccg
     9061 gtacagatcg agccatagcg ctcgcattgc gtggcagtgt cgcttagcca atccagtaac
     9121 cgttgatcgg acgccttgcc gtcgggcagc gcaggtccgc ccgcgaccaa cgccaggttg
     9181 aaagggcgac gcatctgttc aaaggtggcg tcggcgatga gcgccatgcc gttggacgca
     9241 cgtagcggac cgggctccgc gctgaccagc gcgacctcat agcgctcttg cggcgcgacg
     9301 aaccgattgg cttcggagaa tacgtcgacg ggaccggcga catcgagcgc ctgtacgccg
     9361 ggaaagatcg cgatcgcgac ggtaggaatc gaatgatttc ctttgacaat ggacggaatc
     9421 cgcagaccgc ggcagt
//
LOCUS       DATASH010000296         3981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2415542,
            whole genome shotgun sequence.
ACCESSION   DATASH010000296 DATASH010000000
VERSION     DATASH010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2415542"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1911
                     /gene="gspD"
                     /locus_tag="VHV99_08325"
     CDS             <1..1911
                     /gene="gspD"
                     /locus_tag="VHV99_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HEX3635479.1"
                     /translation="IAQIIAGVDTAAGQSVSVMPLKNANALDIAAQLTKMLDPGAIGS
                     TDATLKVSITADPRTNSLLIRASNGGRLAAAKQLAKELDAPTMQLGNMHVVPLRNADA
                     TRLAKTLRGMLGKGGDTGSSGGSNDANSFNQNGGGGGGGGGLGSNSTGTSGTPPLPSG
                     GLGGGSSPMCGSGGGYGSSGGSGSGGLLGGDKDKSDDNQPGGMIQADSATNSLIITAS
                     EPVYRNLRAVIDQLDARRAQVYIEALIVELNSTTNANLGIQWQIANNSVFAGTNLATG
                     NGNSIINLTAAAAGAAATGATGGLAGALATNQLQQGLNVGWIHNIFGVQGLGALLQAL
                     SQTADANVLSTPNLITLDNEEAKIVVGTNVPIQTGSYSNLTSATATSAFNTYDRVDVG
                     LTLHIKPQITDGGILKLQLYTEDSAIVNGTTNATTNPAGPEFTKRSIQSTVLADNGEI
                     IVLGGLMQDNYQVSNSKVPLLGDIPWIGQLFRSEQKTRDKTNLMVFLRPVIITDRETA
                     QAVTSNRYDYIQGVQGAYKSDNNLIKDKDDPVVPPMPLGPAEGGSPAMNLFDLDKMRR
                     EQMAPPGSAPAPVTNGGAVTTNPVMNNGGAVYQTNPVTNGAAVQTNPVTTGGAVQTNP
                     VAPSTASPGVRP"
     gene            1908..3476
                     /gene="gspE"
                     /locus_tag="VHV99_08330"
     CDS             1908..3476
                     /gene="gspE"
                     /locus_tag="VHV99_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HEX3635480.1"
                     /translation="MSTPSTPPSAAASSAAARAAASTQAAAIPPAVVTEHAERSAPSA
                     VAARLVPYGFARSGQILVAHQHADSLEVWISERTNDAALAEVGRNFGALSVVRVPADE
                     LAQAINQAYARQDGSAAQVVGEVEGEVDLSRLMQDIPEVEDLLESEDDAPIIRMINAL
                     LTQAAREQASDIHIEPFETSSVVRFRVDGTLRDVVRPKKALHGALISRIKIMAQLDIA
                     EKRLPQDGRITLRVGGRPVDVRVSTLPTGHGERAVLRLLEKDASRLNLEALGMAPDTL
                     VKFDKLIGKPHGIVLVTGPTGSGKTTTLYASMSRLETATTNIMTVEDPIEYDLSGIGQ
                     TQVNERIGMTFARALRSILRQDPDIIMIGEIRDLETAQIAVQASLTGHLVLATLHTND
                     AASAVTRLTDMGVEPYLLASSLLGVLAQRLVRRLCPVCREERVEQDGSVRWHPVGCDK
                     CGQSGYAGRRGVYELLLIDDEIRTLVHRNASDAEILASGRAQGMRTLREDSDRWLASG
                     LTSLEEVIRVTGGA"
     gene            3481..>3981
                     /locus_tag="VHV99_08335"
     CDS             3481..>3981
                     /locus_tag="VHV99_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX3635481.1"
                     /translation="MPAFRFEAIDAAGKAQKGVLDADSARGARTNLRSQGLTPLVVEP
                     AASRTRGERNQRLSLGRRLSQREQAILTRQLASLLIAGLPLDEALSVLTEQSERDYIR
                     ELMASIRAEVLGGHSLANALTQHPKDFPEIYRALVAAGEHTGKLGLVLSRLADYIEQR
                     NALKQKI"
BASE COUNT          745 a         1259 c         1336 g          641 t
ORIGIN      
        1 atcgcgcaga tcatcgcggg tgtggatacg gctgcgggcc agtcggtctc ggtgatgccg
       61 ctgaagaacg cgaacgcgct cgatatcgcc gcgcagttga ccaagatgct cgaccccggc
      121 gcgatcggca gcaccgacgc aacgctgaag gtgtcgatca cggcggaccc gcgcaccaac
      181 tcgctcctga tccgcgcgtc gaatggcggg cgtctcgcgg cggcgaagca gttggcaaag
      241 gaactcgacg cgccgaccat gcagctcggc aacatgcacg tggtgcccct gcgcaacgcg
      301 gacgcgacga ggctcgcgaa gaccttgcgc gggatgctcg gcaagggcgg ggacaccggc
      361 tcgtcgggcg gctcgaacga cgcgaattcg ttcaaccaga acggcggtgg tggcggcggc
      421 ggcggtgggt tgggcagcaa ttcgacgggt acctcgggta cgccgccgtt gccgtcgggc
      481 ggactcggag gcggttcgtc gccgatgtgc ggcagcggtg gaggatatgg ctcgagcggc
      541 ggttccggct ccggcggcct gctcggcggc gacaaggaca agagcgacga caatcagcct
      601 ggcggcatga tccaggcgga ttccgcgacc aactcgttga tcatcacggc gtccgagccg
      661 gtgtaccgca acctgcgggc ggtgatcgac cagctcgacg cgcggcgcgc acaggtttat
      721 atcgaggcgc tgatcgtcga gctcaattcg accacgaacg ccaatctcgg cattcagtgg
      781 cagatcgcca acaattcggt ctttgccggc accaacctgg cgaccggcaa cggcaacagc
      841 atcatcaatc tgacggcggc ggccgcaggg gccgcggcga cgggggccac cggcggtctc
      901 gccggggcgc tcgcaaccaa tcagctccag caggggctca acgtcggctg gattcacaac
      961 attttcggtg tgcagggact gggcgcgctg ctgcaggcgc tctcgcagac cgctgacgcg
     1021 aatgttctgt cgacacctaa cctcatcacg ctcgacaacg aggaagccaa gatcgtcgtc
     1081 ggcacgaacg tgccgatcca gacaggttcg tattcgaatc tcacgagcgc caccgcgacc
     1141 tcggcgttca atacctacga ccgcgtcgac gtcggcctga cgctgcacat caaaccgcag
     1201 atcaccgacg gcgggattct gaagctgcag ctctacacgg aagactcggc gatcgtgaac
     1261 ggcacgacca atgcgacgac caacccggcc ggcccggagt tcaccaagcg ttcgatccag
     1321 tcgacggtgc tcgccgataa cggcgagatc atcgtgctgg gcggcctgat gcaggacaac
     1381 taccaggtca gtaacagcaa ggtgccgttg ctgggcgata tcccgtggat cggccagctg
     1441 ttccgctcgg aacagaagac acgcgacaag accaatttga tggtgttcct gcgccctgtg
     1501 atcatcaccg atcgcgaaac cgcacaggcc gtgacgtcga accgttacga ctacatccag
     1561 ggcgtgcagg gtgcgtacaa gtcggacaac aacctgatca aggacaagga cgatccggtg
     1621 gtgccgccga tgccgctcgg tcctgccgaa gggggctcgc ccgcgatgaa cctgttcgat
     1681 ctcgacaaga tgcgtcgtga gcagatggcg cccccggggt ccgctccggc gccggtgacg
     1741 aatggcgggg cggtgacgac gaacccggtc atgaacaatg gcggagccgt gtaccagacg
     1801 aacccggtca cgaatggcgc tgctgtacag acgaatccgg tcacgacggg tggcgctgta
     1861 cagaccaacc cggtggcgcc gtccacggcg tcgcccggag tgcggccgtg agcacgccgt
     1921 ccacgccgcc gtcagctgcc gcgtcgtcgg cagcggcgcg ggccgctgcg tcgacacagg
     1981 ccgcggcgat tccgcccgcg gtcgtgacgg agcacgccga acgcagtgcg ccgtcggcgg
     2041 ttgccgcgcg cctcgtgcct tacggcttcg cccgcagcgg ccagattctg gtcgcgcatc
     2101 agcacgccga cagcctcgaa gtctggatca gcgagcgcac caatgacgcc gcgcttgctg
     2161 aggtcgggcg caacttcggc gcgttgtccg tcgtgcgggt gccggctgac gaacttgccc
     2221 aggcgatcaa ccaggcctac gcgcgccagg acggcagtgc ggcgcaggtg gtcggtgaag
     2281 tggaaggcga agtcgatctg tcgcgcctga tgcaggacat tcccgaggtc gaggatctgc
     2341 tcgagtcgga agacgatgcg ccgatcatcc gcatgatcaa cgcgctgctc acacaggccg
     2401 cgcgagaaca ggcatcggat attcacatcg agccgttcga gacttcttcg gtggtgcgct
     2461 ttcgtgtcga cggtacgttg cgtgacgtcg tgcggccgaa gaaagcgttg cacggtgcac
     2521 tgatctcgcg gatcaagatc atggcgcagc tcgatatcgc cgagaagcgt ttgccgcaag
     2581 atggccgcat tacgctgcgc gtcggcggac gtccggtcga cgtgcgcgtg tcaaccttgc
     2641 ccaccggcca cggtgaacgc gcggtgctgc gtctgctgga aaaagatgcg tcgcgcctga
     2701 atctcgaagc gctcggcatg gcgccggata cgctcgtcaa gttcgataag ctgatcggca
     2761 agccgcacgg catcgtgctg gtgacgggcc caaccggttc cggtaagacg accacgctgt
     2821 atgcgtcgat gtcgcgcctc gagaccgcga ccaccaacat catgacggtt gaagacccga
     2881 tcgaatacga cctgtccggc atcggccaga cgcaggtcaa cgagcggatc ggcatgacct
     2941 tcgcgcgggc gctgcgctcg attctgcggc aggatccgga catcatcatg atcggtgaaa
     3001 tccgcgacct ggaaaccgcg cagatcgcgg tgcaggcctc gctgacgggt cacctggtgt
     3061 tagcgacgct gcacacgaac gacgccgcct cggcggtgac gcgtttgacc gacatggggg
     3121 tcgagccgta tctgctggcg tcgtcgctgc tcggcgtgct ggcgcagcgg ctggtgcggc
     3181 ggctgtgccc ggtgtgccgc gaagagcgcg tcgaacagga cggcagcgtg cgctggcatc
     3241 cggtgggttg cgacaaatgc gggcagtccg gctacgcagg ccggcgcggc gtatacgaac
     3301 tgcttttgat cgacgacgaa atccgcacgc tggtccaccg caatgcgtcc gatgctgaga
     3361 ttctcgcgtc gggccgcgcg caaggcatgc gcacgttgcg tgaagattcg gaccgctggc
     3421 ttgcctcggg gctcacgtcg ctcgaagaag tgatccgcgt aacaggcgga gcataagcgc
     3481 atgccggcat ttcgtttcga agcaatcgat gcggcgggca aagcgcaaaa aggcgtgctc
     3541 gacgcggaca gtgcgcgcgg cgcgcgcacc aatctgcgtt cgcaagggct gacgccactg
     3601 gtggtggaac cggcagcgtc gcgtacccgt ggcgaacgca atcagcgctt gtcgctgggc
     3661 cggcgtttgt cgcagcgcga gcaggcgatt ctcacgcgtc aactcgccag cctgctgatc
     3721 gccggcttgc cgctcgacga agcgctttcg gtgctgaccg aacaatcgga gcgcgactac
     3781 atccgcgaac tgatggcatc gattcgcgct gaagtgctgg gcggccattc gctcgcgaat
     3841 gcgctgacac agcatcctaa agacttcccc gagatctatc gggcgctggt ggccgcaggc
     3901 gaacataccg gcaagctcgg tctcgtgctc tcgcgtctgg ccgactacat cgagcaacgc
     3961 aacgcgctca agcagaagat c
//
LOCUS       DATASH010000297         9712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3007290,
            whole genome shotgun sequence.
ACCESSION   DATASH010000297 DATASH010000000
VERSION     DATASH010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 9712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9712
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3007290"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..829)
                     /locus_tag="VHV99_08340"
     CDS             complement(107..829)
                     /locus_tag="VHV99_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX3635482.1"
                     /translation="MGYLLVLCVGLLAGTLSGVIGTGSSMMLMPVLVMLFGPQQAVPI
                     MAIAATMGNFGKILAWWREIDWRACGAYCVTAVPGAALGVRTLLALPPRAVELALGLF
                     FVAMVPTRRWLARRAIKFSLWQLSLIGGVVGFLTGIVVSTGPITVPVFMSYGLVKGAF
                     LATEAAGSLAVYGTKVAVFDHFGALPLHVVIDGLITGSALMIGSFSARLIVVRMSPAA
                     FKLVVDGLMLSSGLSLLWAAGR"
     gene            complement(912..2255)
                     /locus_tag="VHV99_08345"
     CDS             complement(912..2255)
                     /locus_tag="VHV99_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX3635483.1"
                     /translation="MIQVFALIGALFLVALNGFFVAAEFGLVKLRQTRVQSLATAHGM
                     RGRLLAKVHGRLDAYLSACQLGITLASLGLGWIGEPAFAELLTPIFSLLGVESEKLIH
                     GISLFFAFSCISFLHIVVGELAPKSLAIREAEKVSLWAATPLYGFYWAMYPAIWVLNT
                     SANAVLKLTGLDADHGHDSHYSTDELKLILRGRRANVATELGAPEGAYSQDEWNTIAH
                     SLDFSRMTVSDLMRPSHEMIAMRRDLPLRENMQVVARHRFSRYPLFEDAAGERVAGMI
                     HLKDLLLARHAGSTLDDLSRYARPVQYVKPDMPALELFRRFRKGAPHFALVGHKNSKP
                     IGFLTLDNLLGALVGQIHDEFRQGDADWTRMDDGTLMGKGSLPVVSLERALGIDIDEG
                     KAESVGGLVIQALNDLPAEGQRVEFDRFDVVVKKMKGPRIVLVRVYPRTFDDEGG"
     gene            complement(2350..3156)
                     /locus_tag="VHV99_08350"
     CDS             complement(2350..3156)
                     /locus_tag="VHV99_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX3635484.1"
                     /translation="MTTSSTGTTGAQPGVSGFAVSERTAHLRAEAALFMRDHVLSLVS
                     HDLRGPLNAIHSWAYVLERKLDTNDPNSQRAVTGIRNGVDQQVKLLETIVDATRAETK
                     SLALAYARFPLHPLLDETAEEVRSGLARARGVEVTVESQLATEQLNGDRERLAAALWV
                     MLTFAVEASAAGAAVTLAARADASRWYASVTFRQGAAALNDPSVPHLLEAFARQQARE
                     PRDAKRIAWVFALCKRVAEAHGGSFEQSDAPESETATLALHVPLSATAAT"
     gene            3345..3686
                     /locus_tag="VHV99_08355"
     CDS             3345..3686
                     /locus_tag="VHV99_08355"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004537279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX3635485.1"
                     /translation="MSTVTTESGLKYEDIVEGSGAEAVAGKTVSVHYTGWLTDGQKFD
                     SSKDRNDPFAFVLGGGMVIKGWDEGVQGMKVGGTRKLTIPPQLGYGVRGAGGVIPPNA
                     TLVFEVELLDV"
     gene            3721..4515
                     /locus_tag="VHV99_08360"
     CDS             3721..4515
                     /locus_tag="VHV99_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX3635486.1"
                     /translation="MNHAAVAAPCVSLRRYGAIEASDVHDFHQVVLGLDGAMVMAVDG
                     VAQQIDAGSAWLIPAGARHDYAGIGENRQLVLDLPAASLAVPERLFNHARAVTVDASL
                     TQLVHRIAAHAAGGAQGDALDTRRFHWDAAARLGAALVADPGTTMAGTQAPGAGLDFA
                     RIDRWLRVHLSEPLSIADLAAHCGFGMRRFHQLFIEAFGETPHRYLQRLRLDTSLGLL
                     SDPRLSLSDIALEIGFGDQSAYTHAFTRRFGLAPGQWRATQHQSHA"
     gene            4883..5065
                     /locus_tag="VHV99_08365"
     CDS             4883..5065
                     /locus_tag="VHV99_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635487.1"
                     /translation="MEKLALQIAQNSVWNKWVAANSAQTDGCISGTKEGCISVAKAGC
                     ISIAMAKPLPKTGCIS"
     gene            5194..6429
                     /locus_tag="VHV99_08370"
     CDS             5194..6429
                     /locus_tag="VHV99_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635488.1"
                     /translation="MENQPTVDVIEQVKDIPIYQQSIAERFFPILEKPAIALDFPDNW
                     MTPRLAAALKLGEAEFVLSTGTHHARMNIIRPPYFLLNSYYQLWSKHPDIQFTWQQQC
                     QRVSLTTVLATEHVARVNARKYGKHIVETASSAMRRLDARTSYLNQKLDPARWERADI
                     ERMLDDIEAAQHCHPHGFYHLDCSSYHLAHFVLKVVQYGLEARFAQPASIVHAYEYTP
                     ANVRWFLMKNFSCPLIDLFGSTELGYLYYSDRCGNYSPYLDKMHLELVPFATSDTIFS
                     LIVSSVRNPYMPLIRYRSGDCVQTGDGSADPEKIVRFCGREKEMLRTSHGIVSQADFD
                     DLICAASPNIFVYQLHPEEKWQACLHYTTFNGAPLLPQEAADLQRRIQEATGMECIPL
                     HRTRIDIGKSGKYAWLAKK"
     gene            6477..6989
                     /locus_tag="VHV99_08375"
     CDS             6477..6989
                     /locus_tag="VHV99_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635489.1"
                     /translation="MHSTLSAPTIDAKTLLGHGLYSEVILYFIKRTGSSSDYVERQVT
                     ECLRYLYLVSKHREQLSGLFLPVEQQIDDIWHYLILQTREYRTLCEERLPGGFFIEHR
                     SCPYATYQQEPSREQLIEQSLRWLPLYRESFGPFDEAALPHWTMPRFLRDEMGLSLSE
                     IAQLEARKTA"
     gene            6986..8458
                     /locus_tag="VHV99_08380"
     CDS             6986..8458
                     /locus_tag="VHV99_08380"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3635490.1"
                     /translation="MKADAMSGYEDAQHQGSERASALALAVILACQLMIVLDGTVIYA
                     ALPHLRADLGLARPDLSWVQNAYMLTFGGFMLLGARAGDILGHRRVFLAAGAAFVLAS
                     CAAGLALSLPQLLIARGVQGIAGAFAAPSALALLMLCFPEGASRARAIRLYTAVSGGG
                     SAAGLVLGGVLTDFASWRWVLFINVPVGTLLLMAGLRCLPKAPRNRGHFDCMGALTVT
                     LGTSLLVYGLVRGVPAGAGDPTTVAALSAAGFLLMAFILIEKRARQPITPLALFANAQ
                     RSGAYVGRMLLIGGMLGTFFFLTQYVQEVLEFSAFRAGLAFLPLSVTQCLMVIVGVPR
                     LMPRLGGKGVLTGGLVIAAVGMMCLSQVDEGTEFFADLALPIMMLGIGSGAALVPLTT
                     AGIDGVAPRDAGAASGLINATHYIGGAMGTALVVFVVDLTLSRAASAPTFTGSASERG
                     AKAELTHALSVSAATSAALFMLALLIVLVTMRRPRPLPQA"
     gene            complement(8490..9680)
                     /locus_tag="VHV99_08385"
     CDS             complement(8490..9680)
                     /locus_tag="VHV99_08385"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA];
                     GO_process: GO:0071268 - homocysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HEX3635491.1"
                     /translation="MDDSLNFDTLAVRSGTARSDFNEHSEAIFLTSSFVFASAADAAE
                     KFKNSEDNYTYSRFTNPTVSMFQDRLAALEGGEACMATASGMAAIMSVVMSSLQAGDH
                     LVSSQALFGSTLGMFSQIFSKFGITTTFVDPTDLDAWKNAVRPETKMFFLETPSNPLT
                     EVADIEAISKIAKAANAIFVVDNCFCSPALQQPLKFGADVVMHSATKFLDGQGRVLGG
                     ALVGSKKFIMEKVFPFVRSAGPTLSAFNAWVLLKGMETLSLRVEKQSANALEIARWLE
                     THPAVNRVFYPGLESHPQHALAMRQQKAGGAILSFELKGDTPEQMRANAWRVIDSTKI
                     CSITGNLGDTRTTITHPATTTHGRVTPEARAAAGISEGLIRLAVGLENAADIRGDLER
                     GLAG"
BASE COUNT         1927 a         3059 c         2852 g         1874 t
ORIGIN      
        1 ggccggattt tcccaggcgg aagtggcagc ggttctgggg ggcaattacc agcgggtgtt
       61 cgagacctcg gtggggtaac cttgtatgcc tgtttgcaaa ccgaccctac cgccctgccg
      121 cccacagcaa tgaaagcccc gaagacaaca tcaacccgtc aaccaccagc ttgaaagccg
      181 cggggctcat ccggaccacg atcagccgcg cggaaaagga cccgatcatg agtgccgaac
      241 cggtgatcaa accatcgatc accacatgca acggcagcgc gccgaaatga tcgaaaaccg
      301 cgactttggt gccgtaaaca gccagtgatc cagctgcttc cgtcgctaga aaagcgcctt
      361 tcacaaggcc atacgacata aagacaggca ccgtgatcgg cccagtggaa acgacgatgc
      421 ccgtgagaaa accgaccaca ccgccgatca acgaaagctg ccacaaagaa aacttgattg
      481 cccgccgcgc cagccagcgc cgcgtaggca ccatcgcgac gaaaaacagc cccaacgcga
      541 gttcgaccgc acggggcggc aacgcaagca gcgttcgcac acccagcgcc gcgcccggca
      601 ccgcagtcac gcaataagcg ccgcaagctc gccagtcgat ctcgcgccac cacgcaagaa
      661 tttttccgaa gttgcccatg gtcgcggcga tcgccatgat cggcaccgct tgctgcggac
      721 cgaacagcat gacgagaacg ggcatcagca tcatggacga gcccgtgccg atcacaccgc
      781 tcaacgtgcc ggccagcaaa ccgacgcaca acaccaacag atatcccatg tcgccccgcc
      841 gtggtatgga cgacgatttt actcgagcaa cgattcagtg gacttggggc gggcctggca
      901 ttagaagccg ctcaaccgcc ttcatcgtcg aaggtccgcg gataaacgcg caccagcacg
      961 atccgcgggc ccttcatctt tttgaccacc acgtcgaagc gatcgaactc gacgcgctgc
     1021 ccttcggcag gcaggtcgtt gagcgcctgg atcacgaggc cgccgaccga ttcggccttg
     1081 ccttcgtcga tgtcgatccc gagcgcgcgt tctaacgaca ccaccggcaa gctgcccttg
     1141 cccatcagcg tgccgtcgtc catgcgcgtc cagtcggcgt cgccctggcg gaattcgtcg
     1201 tgaatctgcc caaccagtgc gccaagcaga ttgtccagcg tcagaaagcc gatcggcttc
     1261 gaattcttgt gcccaaccag cgcgaagtgc ggcgcgccct tgcggaagcg gcgaaacagt
     1321 tcgagcgcag gcatgtccgg cttcacatac tgcacaggcc gcgcatatct ggagaggtcg
     1381 tcgagcgtac tgcccgcgtg ccgtgccagc agcaaatcct tcaaatggat catgccggcc
     1441 acgcgctcgc cggcagcgtc ttcgaacagc gggtagcggc tgaagcggtg ccgcgccacc
     1501 acctgcatgt tttcgcgcaa cggcaaatcg cgccgcatgg cgatcatttc gtgggacggg
     1561 cgcatcagat ccgagacggt catgcgcgaa aaatccagcg aatgcgcaat cgtgttccat
     1621 tcgtcctgac tgtatgcgcc ttccggcgca ccgagctcag tcgcgacatt ggcgcggcgg
     1681 ccgcgcaaga tcagcttgag ttcgtcggtc gagtaatgcg aatcgtggcc gtggtccgcg
     1741 tcgaggcctg tgagtttcaa caccgcgttg gcacttgtat tgagcaccca gattgccgga
     1801 tacatcgccc aatagaaacc gtacagcggc gtggcggccc acagtgagac cttctccgcc
     1861 tcgcgaatcg ccagcgactt cggcgccagc tcgcccacca cgatgtgcag gaacgaaatg
     1921 cacgagaacg cgaagaacag cgagatgccg tgaatcagct tctccgattc cacgccaagc
     1981 aggctgaaga tgggcgtgag cagttccgcg aatgccggct caccgatcca gccgagcccg
     2041 agcgacgcca gcgtaatacc gagctgacag gcggacagat acgcgtcgag ccggccgtgc
     2101 actttcgcca gcaaacgtcc gcgcatgccg tgcgcggtcg cgaggctctg cacgcgcgtc
     2161 tggcgcagct tcaccagacc gaattcggcg gcgacgaaaa aaccgttgag ggcaacgaga
     2221 aacaacgcac cgataagggc gaaaacctgg atcaaagcga agggctccgg caacaaaagt
     2281 tgtcagtata gagggtgaaa gctcattgaa agttaatcgc gtacggcaac tcgcgccaac
     2341 aatggccgct tacgttgctg cggttgcact caacggcaca tgcagcgcga gtgtagccgt
     2401 ttcgctttca ggcgcatcgc tttgctcgaa actgccgccg tgcgcttccg caacgcgctt
     2461 gcacagcgcg aacacccacg cgatccgttt agcgtcgcgc ggttcacgcg cctgttggcg
     2521 ggcaaaggct tccagcaaat gaggcaccga agggtcgttc aaggcagcag cgccctgcct
     2581 gaaggtgacg ctggcgtacc acctggaggc atccgcgcgc gcagccagcg taaccgcggc
     2641 gcccgcggcg ctcgcttcga cggcaaacgt gagcatcacc cacagcgcgg cggccaggcg
     2701 ttcacggtcg ccgttgagct gctcggtagc gagttgcgac tccaccgtga cctcgacgcc
     2761 acgcgcgcgt gccaagccgg agcggacttc ttccgctgtc tcgtcgagta gcggatgcag
     2821 cggaaaccgc gcatacgcga gcgccagcga cttggtctcg gcccgtgtgg cgtcgacgat
     2881 cgtctccagc aacttcacct gctggtccac gccgttgcga attccggtca cggcccgctg
     2941 cgagttgggg tcgttcgtat cgagcttgcg ctcgagcaca tacgcccaac tatggatggc
     3001 gttcagcgga ccccgcaggt cgtgcgacac cagcgacagc acgtggtcgc gcatgaacag
     3061 cgcagcctcc gcgcgcagat gtgcggtgcg ttcggatacg gcgaagccgg aaacacccgg
     3121 ctgtgcgccg gtggttccgg tggaagacgt tgtcacgatc gagccctctc aggcagtgcg
     3181 taaaatatcg aagaagcccc attataggca tgctgtccgg cgccacccgc ctggccggac
     3241 ggctaacagg gacgcaaacg acatgccacg cggcaatcct cggcgatgcg acgccaacac
     3301 gcctacaatg tcggttttta cgtttttgaa ccaaggagca acacatgtcg actgtgacta
     3361 ccgaatccgg cctgaaatac gaagacatcg tcgaaggcag cggcgccgaa gccgttgccg
     3421 gcaagaccgt cagcgtgcac tacacgggct ggctgaccga cggccagaag ttcgactcga
     3481 gcaaggaccg caacgacccg ttcgcgttcg tgctgggcgg cggcatggtc atcaagggct
     3541 gggacgaagg cgtgcaaggc atgaaggtgg gcggcacgcg caagctgacg attccgccgc
     3601 aactcggcta cggcgtgcgt ggcgcaggcg gcgtgattcc gccgaatgca acgctcgtgt
     3661 tcgaagtcga actgctcgac gtctaagctt tcccctgttt tgtaatcggg tgcattgccg
     3721 atgaatcacg ccgccgttgc cgctccttgc gtttcgttgc gccgctacgg cgcaatcgag
     3781 gcgtcggacg tgcacgactt tcatcaggtc gtgctcggac tcgacggcgc gatggtgatg
     3841 gcggtggacg gcgtcgcgca acagatcgac gccggttccg cctggctgat cccggcaggt
     3901 gcacggcacg actatgccgg tatcggagag aaccggcagt tggtgctgga tctgccggcc
     3961 gcctcgctgg ccgtgccgga acgtctgttc aaccacgcgc gggccgtgac ggtggatgcc
     4021 tcgctcacgc aactcgtgca tcggattgcg gcgcacgccg cgggcggtgc gcaaggcgac
     4081 gctctggata cgcggcgttt tcattgggac gcggccgcac gcctgggtgc tgcgttagtg
     4141 gcggaccccg gcacgacgat ggcaggcacg caagcgcctg gcgctggtct ggatttcgcg
     4201 cgcatcgacc gctggctgcg cgtgcatctg tcggaaccgc tgagtatcgc cgacctcgcc
     4261 gcccattgcg gcttcggcat gcggcgcttt catcagcttt ttatcgaagc cttcggtgaa
     4321 acaccgcatc gctatttaca gcgtctgcgg ctcgatacgt ctcttggctt gctgtccgat
     4381 ccacgcttgt cgctcagcga tatcgcgctg gaaatcggct tcggcgatca gagcgcctac
     4441 acgcatgcgt tcacgcggcg tttcgggctg gcgcctggac agtggcgtgc gacacagcat
     4501 caaagtcacg cttgaaccgc ggtccccgta ctgttaccaa cagttaaatc tggcaaacaa
     4561 agcagtacgg atcagatgtg tcattgcgtc acacgcgcca ggcaaaagcg cgacgagtct
     4621 tcgcgactga cagtcatcac ttcatccctc cgcttcccct ggcagccctt tgcatcaagg
     4681 cctttggcaa accacatctc aatctcaccg tagacgtcga gtctgcgcgc ggatgtgcga
     4741 ccgcccgatc gtatcgtcgc accgcacaaa taaaaatgaa ccgacctcag aagcgaaaga
     4801 cagagatacg gggtgcgtac tattttttgc tgttctgttg acgcactcca atcccaacgc
     4861 tttacacctt tggaggtaca cgatggaaaa gctggctctt caaattgctc agaactccgt
     4921 atggaataag tgggttgccg ccaactccgc tcagacagac ggctgcatct ccggcaccaa
     4981 ggaaggttgt atttccgttg cgaaggcagg ctgcatttca atcgccatgg cgaagccact
     5041 gccgaaaacc ggttgtatca gctaatcgcc ctacgtcgaa catgagctga cagatcaggt
     5101 aaaacacgct cacctggcca gctcatcagg ctggtccttg cacaccacaa ggaccagctt
     5161 ccctcgacat cgcaaccagg ctacggagcg catatggaaa accagcccac agtcgatgta
     5221 atcgagcagg tcaaagacat ccccatctat caacagtcga tcgcggagag attcttcccc
     5281 attctggaaa aaccggcaat cgcactggac ttccccgaca actggatgac gccgcgactc
     5341 gccgcagcgc tcaagctagg cgaagccgag ttcgttctct cgaccggcac ccatcacgcg
     5401 aggatgaata tcatccgtcc tccttatttt ctattgaact cgtactatca actgtggagc
     5461 aaacatcccg atatccagtt cacatggcag caacagtgcc aacgcgtatc gctcactacg
     5521 gtgctggcga ccgaacacgt ggcgcgagtg aacgctcgaa aatacggcaa gcatatcgtt
     5581 gaaacggctt catccgcgat gcggcggctc gatgcgcgca cctcgtattt gaatcagaag
     5641 ctcgatccgg cacggtggga acgcgccgat atcgagcgca tgcttgacga tatcgaggcg
     5701 gcccagcatt gccatccgca tgggttttac catctggatt gttctagcta ccatctggcg
     5761 catttcgtat tgaaggtcgt gcaatacgga cttgaagcac gctttgccca acctgccagc
     5821 atcgtccatg cctacgagta caccccggca aacgtccggt ggttcctgat gaaaaatttc
     5881 agttgcccgc tcatcgatct cttcggcagc accgaactcg gctatctcta ctacagcgat
     5941 cgatgcggaa actactcacc gtacctggac aaaatgcacc tggaactcgt gccgtttgcg
     6001 acgagcgaca caatcttcag cctgattgtg tcgagcgtgc gtaatcccta tatgccgctg
     6061 atccgctacc gctccggcga ttgcgttcag accggcgacg gctcggcgga ccccgaaaaa
     6121 attgtccggt tttgcggccg cgaaaaggaa atgctgcgga cttcgcacgg cattgtgtcg
     6181 caagcggatt ttgacgatct gatctgcgct gcttcgccga atatcttcgt gtaccaactg
     6241 catccggagg agaagtggca agcctgcctg cattacacga cgttcaatgg cgccccgctc
     6301 ctgccgcagg aagccgctga tctgcaaagg cgtattcagg aagcgacggg gatggaatgc
     6361 attcccttgc atcgcacgcg tatcgatatc ggcaagtcgg gcaaatatgc ctggctcgcg
     6421 aaaaaataag gatcacccaa cacgttccga tcgcttccct gcaacgagga caacgcatgc
     6481 attccaccct ttctgctccg accatcgacg caaagacatt gctcggtcac ggtctctatt
     6541 cagaagtaat cctctatttc atcaagcgca ccgggtcgtc ttcggactac gtggagcggc
     6601 aagtcacgga atgcctgcgc tatctttatc tggtgtcgaa gcatcgcgaa caactgagtg
     6661 gtcttttctt gccggtcgag cagcaaatcg acgatatctg gcattacctc attctccaga
     6721 cacgcgaata ccggacactt tgtgaagaac gcttgcccgg cggcttcttt atcgaacacc
     6781 gcagttgccc ttacgcgacc tatcaacagg agccgagccg tgagcaactg atcgagcagt
     6841 cgcttcgctg gctaccgctg taccgggaat cctttggtcc cttcgacgaa gccgcgctgc
     6901 cgcattggac catgccacgt tttcttcgcg acgaaatggg cctctcattg tcggagatcg
     6961 cgcagctcga ggcccgcaag acagcgtgaa ggccgacgcc atgtcaggct acgaggacgc
     7021 ccagcatcag ggaagtgagc gagcaagcgc gcttgccctt gcggtcatcc tggcctgcca
     7081 gttgatgatt gtgctcgacg gaaccgtgat ctacgcggca ctgccgcatc ttcgggcaga
     7141 cctcgggctt gcgcgcccgg atctgtcctg ggtgcagaac gcctatatgc tgactttcgg
     7201 cgggttcatg ctgctgggcg cccgcgccgg cgacattctc ggacaccgcc gcgtcttcct
     7261 cgccgccggc gcggcgtttg tcttggcgtc gtgtgccgct gggctcgcgc tgtccctgcc
     7321 gcagctactc attgcgcgcg gcgtgcaagg catcgctggc gcctttgccg cgccttccgc
     7381 gctcgcgctg ctgatgcttt gtttccccga aggcgcatcg cgcgcgcgtg ctatcagact
     7441 ctacacagcg gtatccggcg gcggcagcgc agcggggttg gtgctcggcg gcgtcctcac
     7501 ggattttgcc tcctggcgct gggtattgtt catcaatgtg cccgtcggca cgcttttgct
     7561 aatggccgga ctgaggtgtt tgcccaaagc gccgcgcaac cgcgggcatt tcgactgcat
     7621 gggcgcattg actgtcaccc tcgggaccag tcttctggtg tatgggctcg tgcgtggcgt
     7681 gccggccggg gcgggagacc ccaccaccgt ggcagcgctc agcgcagcag ggttcttgct
     7741 catggccttc attctcatcg aaaagcgggc gcgccaaccg atcactccgc tcgcgctctt
     7801 cgccaacgcg caacgttcag gcgcttacgt cggcaggatg ctgttgatcg gcggcatgct
     7861 gggaaccttc tttttcctga cccaatacgt gcaggaagtc ctggaattca gcgcgttccg
     7921 cgcagggctc gccttcctgc cactgtcggt cacgcagtgt ctgatggtga tagtcggggt
     7981 gccacgtttg atgccacggc tgggcggcaa aggcgtgttg acaggaggac tcgtgatcgc
     8041 cgcggtcggc atgatgtgcc tgagccaggt ggatgaaggc accgagtttt tcgccgatct
     8101 ggcgttgccc atcatgatgt tgggaatcgg ctccggtgcc gcactggtac cgcttacgac
     8161 agccggcatt gacggcgtgg cgccgcgtga tgccggcgca gcgtccggtc tgatcaacgc
     8221 gacgcactat atcggcggcg cgatgggcac ggcgcttgtc gtcttcgttg tcgatctgac
     8281 gcttagccgg gccgcttcag cgcctacgtt caccggttcg gcttcggaac ggggtgccaa
     8341 ggctgaactt acccatgcgc tgtcggtttc cgctgccaca tccgcggcgt tgtttatgct
     8401 ggcgctgctg atcgtgctgg tcacgatgcg ccgcccgcgc ccactgccac aggcataagc
     8461 gttcaggcgg cgacccgttc ctccgccgct taacccgcca gaccgcgctc cagatcgccg
     8521 cggatatcgg cggcgttttc cagacccact gcaagacgga tcaagccttc gctgataccc
     8581 gccgctgcgc gcgcctcggg cgtcacgcga ccgtgcgtgg tggttgccgg atgcgtgatg
     8641 gtagtgcgcg tgtcgccgag gttgccggtg atcgagcaga tttttgtgct gtcgatcacg
     8701 cgccacgcat tcgcgcgcat ctgctcaggc gtatcgccct tcagttcgaa cgacaggatt
     8761 gcgccgcccg ccttctgctg acgcatggcc agcgcgtgct gcggatgcga ttcgagcccc
     8821 ggataaaaca cgcggttcac ggccggatgc gtttcgagcc agcgcgcgat ttccagcgca
     8881 ttcgccgact gtttttcgac gcgcagcgac agcgtttcca tgcccttcaa caacacccat
     8941 gcattgaatg cggacaaggt cggccctgcg ctgcgcacga acgggaacac cttttccatg
     9001 atgaatttct tcgagccgac cagcgcgccg ccgagcacac ggccctgccc atcgaggaac
     9061 ttggtggccg agtgcatcac aacgtccgcg ccgaacttca gcggctgttg caacgccggg
     9121 ctacagaagc aattgtcgac cacgaagatc gcattcgctg ccttcgcgat cttactgatc
     9181 gcttcgatat cagcgacttc ggtcagcggg ttcgacggcg tttcgaggaa gaacatcttc
     9241 gtctccgggc gcacggcgtt tttccacgca tccagatcgg tcggatcgac gaaggttgtc
     9301 gtgatgccga acttgctgaa gatttgcgag aacatgccga gcgtcgaacc gaacagcgct
     9361 tgggagctga ccagatggtc gccggcctgc aacgaggaca tcaccaccga catgatcgcg
     9421 gccatccccg aagccgtggc catgcacgct tctccgcctt cgagcgcggc cagacggtcc
     9481 tggaacatcg agacggtcgg gttcgtgaag cgcgagtaag tgtaattgtc ttcggaattc
     9541 ttgaattttt cggcggcgtc cgcggcactc gcgaagacga agctcgaggt caggaaaatc
     9601 gcttccgagt gttcgttgaa gtcgctgcgc gctgtgcccg agcggactgc cagcgtatcg
     9661 aagttcaggg agtcgtccat gttggttctg gtttccaatg ttccatggct gg
//
LOCUS       DATASH010000298         5879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6963910,
            whole genome shotgun sequence.
ACCESSION   DATASH010000298 DATASH010000000
VERSION     DATASH010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5879
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6963910"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..146)
                     /locus_tag="VHV99_08390"
     CDS             complement(<1..146)
                     /locus_tag="VHV99_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HEX3635492.1"
                     /translation="MHIRFDAADAHWRVAPLPRFSAAQKDWLTRGGSLTAHLRTLGEV
                     AVRVT"
     gene            392..898
                     /locus_tag="VHV99_08395"
     CDS             392..898
                     /locus_tag="VHV99_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX3635493.1"
                     /translation="MRTFLGATACAVSVLLMAGCAAPLNRDTETSLNKAVGIEVAPVA
                     GGVSVKLPEAALFEFGKSEVRGDAAMVINRSAVLLKRSKKPIVVEGYTDNVGTLEYNQ
                     KLSEDRATAVGYALVARGVALERIRTKGNAYNSPVASNDTAEGRALNRRTEILVRGET
                     MDTLMGKK"
     gene            complement(972..2777)
                     /locus_tag="VHV99_08400"
     CDS             complement(972..2777)
                     /locus_tag="VHV99_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX3635494.1"
                     /translation="MSDSVLAKSADPVHLLWSILGEQGFDAAARSAVEFATQGMTPQL
                     HADLCASAGRFEEAYHSASQAPAVTRGARHARLALFADALGHAEAAQRNSELAVVAQS
                     QAATIEWIVWLIDHCGAHSAAAHMVRAYEQHVPQDARAPWWLAISLAAQTAASSRAER
                     RQALLRAYALDPSTHPALPLQLVLALRESRDWPAVERVCREMLSRNPADAEMAWQLSH
                     AQWQCNDAAAAEATMRAVDAAAPGNADVLAAIGMYLAEQARYDESEAALHAALVCDPS
                     TTQAAVDLADLKLRRGAWSSGWPHLEARLTRDDRESNNVVTLMAQLCPRWRGEPLAGK
                     TLVVHSEQGSGDDIQMVRFVPELAARVRAEGGRVMLAVRRPLQALFARYYADCVSIEA
                     GAPGKPHYDYGLPMMSVLSVLGLPPEQVHGAPYLHADAAKVAAWRERVSGCGSDAVLH
                     VGLVWSGSPTHRRDAKRSIPLAALTPMLAVSNVVFHALTPGRHADVAALTAQGHRVSD
                     LTACYQAGFDDVAAHVSALDAVVTIDSAPLHLGGALGRPVMAMLDHVSHWCWGNEEAQ
                     PWYDSVDLFRQPRPGAWEPVVERVAARLRAMAGAR"
     gene            3059..3328
                     /locus_tag="VHV99_08405"
     CDS             3059..3328
                     /locus_tag="VHV99_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635495.1"
                     /translation="MNTTNSKETSDRIAHLAAVVLRRTSASHQEKELAGSALAQHHTA
                     KQTSAAIGSLAAKVLADHHSCMEAKELAGSVLSQVRHLHGLAVVR"
     gene            complement(3489..5387)
                     /gene="htpG"
                     /locus_tag="VHV99_08410"
     CDS             complement(3489..5387)
                     /gene="htpG"
                     /locus_tag="VHV99_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HEX3635496.1"
                     /translation="MAQETMSFQAEVKQLLQLMIHSLYSNKEIFLRELISNASDAADK
                     LRFEAIENSALYENDSNLRIRVSYDKAARTVTVDDNGIGMSRDEAIAHLGTIARSGTK
                     EFFGKLSGDQQKDAALIGQFGVGFYSGFIVADRITVETRRAGLPASEGVRWESAGEGD
                     FAVETIERAARGTTITLHLRADEDDLLSSHRLKSIIQKYSDHVALPILMAKEEWDAEK
                     SEMVTKDEDETVNQASALWTRSKNDITEEQYKQFYQHLSHDHQDPLTWTHNRVEGRSE
                     YTQLLYVPTHAPFDMFNRDHRGGLKLYVKRVFIMDDAEQLLPAYLRFVKGVVDSADLP
                     LNVSREILQESRDVKAIREGVTKRSLSMLEELANSDNEADKEKYAGFWKEFGQVLKEG
                     IGEDFANRERIAKLVRFASTHTETPEQTVSLADYVSRMKPEQTKIYYVTADTWQAATH
                     SPHLEVFRKKGVEVLLLTDRVDEWMLSFLNEFDGKPLQSVARGDLDLGALNDEEKQAQ
                     EKVGEAFKPLVDKMKEALKDKAKDVRLTFRLTDSPSCLVADDGEMSGYLQRMLKAAGQ
                     DAPSFHPILEVNPEHALVKGLHADSANFDDWCHLLFDQALLAEGGALEDPASFVKRTN
                     ALLLARAG"
     gene            complement(5493..>5879)
                     /locus_tag="VHV99_08415"
     CDS             complement(5493..>5879)
                     /locus_tag="VHV99_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004533687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX3635497.1"
                     /translation="LVGMTSHELNERVIYKILTEGHYRRHVERIRARLDGVREKAVRM
                     IEKTGMKLFLTPTAGMFLWADSGVHAGALAAAAHEAGFLLTPGGLFSPQQSPSTWMRF
                     NIANCGDPELPAFLSRYLDGVARRAS"
BASE COUNT         1150 a         1891 c         1750 g         1088 t
ORIGIN      
        1 gtcacacgca cagcgacttc gcccagcgtg cgcagatgcg cggtcagcga tccaccgcgg
       61 gtcaaccagt ctttctgggc ggcgctgaag cgaggtaagg gcgcgacgcg ccagtgggcg
      121 tcggcggcat cgaaacggat atgcatggtg tcggattata gcggcgcggc cgtcggagca
      181 ttgcctagcg ctcacctatc tccccttcag gaagttccac tgacatctgt ctgaatgctg
      241 actcgcctca aacagattat cgatttcaga gtgttccgat tctcccgtgc cagcgacgcg
      301 cgatacaacc ttcgtgcttt acacagtggg cggctcgcct gagatcgaaa aggcgcgcca
      361 tttgatcgga tagagaagga gaagaagaaa catgagaacg ttcctgggcg caacagcatg
      421 cgcggtgtcg gtcctgctga tggcgggttg cgcggcaccg ctcaatcgcg atacggaaac
      481 atcgctgaac aaggcagtcg gcatcgaggt ggcccccgtc gccggcgggg tatcggtgaa
      541 gctgccggag gcggcgctgt tcgaattcgg caaatccgaa gtacgcggcg acgcggccat
      601 ggtgatcaac cgctccgcgg tgttgctcaa gcgcagcaag aaaccgatcg tggtggaggg
      661 atacaccgat aacgtgggca cgctggaata caaccagaag ctctcggagg accgcgctac
      721 cgcggtgggt tacgcgcttg tcgcgcgcgg cgtggcgctg gagcgcatcc gcacgaaggg
      781 caacgcgtat aacagtccgg tggcaagcaa cgacacggct gagggtcgcg cactgaaccg
      841 ccgcaccgag atcctcgtgc gtggcgaaac gatggatacg ctgatgggta agaagtaggc
      901 gttcgccagg ctcgtaacgt aaaaccgccc gcgtcatgcg ggcggtttta cgattcacgc
      961 aagcagagta actatcgcgc gccagccatc gcccgcaaac gggccgccac gcgctccacc
     1021 accggctccc acgcccccgg ccgcggctgc ctgaacagat cgaccgagtc gtaccacggc
     1081 tgtgcttcct cattgcccca acaccagtgc gacacatgat cgagcatcgc cataactgga
     1141 cgccccaacg cgccgcccag atgcaacggc gcgctgtcga tggtgaccac ggcatcgagc
     1201 gcgctcacgt gcgcggccac gtcgtcgaaa ccagcctggt agcatgccgt cagatcgcta
     1261 acgcgatggc cctgcgcagt caaagccgcc acgtcggcgt gacggcccgg ggtcagcgca
     1321 tggaacacca cgttggatac agcgagcatc ggagtcagcg ccgcaagcgg aatggagcgc
     1381 ttggcgtcgc gccgatgcgt cgggctgccc gaccacacca gaccgacatg caacacggcg
     1441 tcgctgccgc acccgctgac acgctcgcgc cacgcggcca ctttggccgc gtcggcatgc
     1501 agatatggcg cgccgtgcac ctgctcaggc ggcaaaccga gaacggatag cacgctcatc
     1561 atcggcaggc catagtcata gtgtggtttg ccgggcgcgc ccgcttcaat cgacacgcaa
     1621 tccgcgtaat agcgcgcaaa cagcgcctgc aacgggcggc gcactgcaag catcacgcgg
     1681 ccgccctcag cgcgcacacg cgccgccaat tccggcacga agcgcaccat ctggatgtcg
     1741 tcgccgctgc cctgctcgct gtgcacgacc agcgtcttgc cggcgagcgg ttcgcctcgc
     1801 cagcggggac acagctgtgc catcaacgtg accacattgt tcgactcgcg gtcgtcgcgc
     1861 gtcaatcgcg cctccaaatg cggccatccg ctcgaccatg cgccgcgccg caatttcaga
     1921 tcggccagat cgaccgccgc ctgcgtcgtc gaaggatcgc acacgagcgc ggcatgcagt
     1981 gccgcttcgc tctcgtcata gcgtgcctgt tcggcgagat acatgccgat cgcggccagc
     2041 acgtcggcat tacccggcgc ggcggcatcg acggcacgca tggtcgcctc ggccgccgcg
     2101 gcgtcgttgc attgccattg cgcgtggctc agttgccagg ccatttccgc atcggcggga
     2161 ttgcgcgaca gcatttcgcg gcaaacgcgt tcgaccgcgg gccaatcgcg actctcgcgc
     2221 aaggcgagca cgagttgcaa cggcagcgcg gggtgcgtcg acggatcgag cgcataggcc
     2281 cgcaacagtg cctggcgacg ctcggctcgc gaactcgccg ccgtttgtgc tgccaacgaa
     2341 atcgccagcc accacggcgc gcgcgcgtcc tgcggcacat gctgctcata cgcgcgcacc
     2401 atgtgcgccg ctgccgaatg cgcgccgcaa tgatcgatca accagacaat ccactcgatc
     2461 gtcgcagcct gcgactgggc aaccaccgcc agttcgctgt tgcgctgcgc ggcctcggca
     2521 tggccgagtg catcggcgaa gagcgccagc cgggcatgac gcgcgccacg tgtcacggcc
     2581 ggcgcttgcg acgcgctgtg ataggcctct tcgaagcggc ccgcgctcgc gcacaagtcc
     2641 gcgtgcaact gcggcgtcat gccttgtgtc gcgaactcga cagcgcttcg cgcagcggca
     2701 tcgaagcctt gctcgcccaa gatggaccac aacagatgaa caggatcggc ggatttcgcc
     2761 aagacagagt cggacatagg agatacgaga agtgagcgca acggcgggtt gcgtgaatgc
     2821 gtcatcgcgg aaataagact cggtgtttca ttggcctaga gattgcaaac aatcctctaa
     2881 acctccgagt ttcgaacgcg cgagcataag acaaatcggc gcttttacat ctcataaacc
     2941 aatctcaatt tatggttgtt gaagagtttt atttagttaa ccataattaa ttttcaggtg
     3001 aatctttccg gcaggaagct ttgcctgaat cgacaaaaaa cacataggag taaagggctt
     3061 gaatactacg aactcgaaag aaacttcaga ccgcatcgcc catctggctg cggtcgtgtt
     3121 gcggcgcacg agtgcttcgc atcaggaaaa agaactcgcc ggctcggcgc tcgcacagca
     3181 tcacacggcg aagcaaacca gtgctgccat cggcagtctc gcggcaaagg tcctggccga
     3241 tcaccattcc tgcatggagg ccaaggagct agccggttcc gtactgtcgc aggtgcgcca
     3301 tttgcatggg ctggcggtgg tgcgttaaaa gcgctccacc gcctccatct aaaccggtgc
     3361 aaaacgcacc gggagcacga aagcgatcgg caaaaaaaat gcgctgccaa ggcagcgcat
     3421 ttttcatcta caagcgtcga atcgacgccg gcgcaagctc aactcaaact cgattcaaac
     3481 tcgaaaactc aacccgcacg cgccaacaac aacgcgttcg tacgcttcac gaagctagcc
     3541 ggatcttcca gcgccccacc ttcagcgagc aatgcctgat cgaacagcag atggcaccag
     3601 tcgtcgaaat tcgcgctatc cgcatgcaag cctttcacca gcgcatgctc cggattcact
     3661 tcgagaatcg gatggaacga cggcgcgtcc tgacccgccg ccttcagcat acgctgcaga
     3721 tagccgctca tctcgccgtc gtcggcgacg agacaggacg gcgaatcggt caaacggaac
     3781 gtcagacgca catccttggc cttgtccttc agcgcttctt tcatcttgtc gacgagcggt
     3841 ttgaacgctt cgccgacctt ctcctgcgcc tgcttttcct cgtcgttcag ggcgcccaga
     3901 tcgagatcgc cgcgcgcgac actttgcagc ggcttgccgt cgaactcgtt caggaacgac
     3961 agcatccatt catcgacgcg gtcggtcagc aacagcactt ccacgccctt cttgcggaac
     4021 acctcgaggt gcgggctgtg ggtcgcggcc tgccaggtat cggcggtcac gtagtagatc
     4081 ttggtctgct cgggcttcat gcgcgaaacg taatcggcga gcgaaaccgt ttgctccggc
     4141 gtttccgtgt gcgtcgacgc aaaccgcacc agcttcgcaa tacgctcgcg attggcgaag
     4201 tcttcgccga tgccttcctt cagcacctgg ccgaattcct tccagaaacc ggcgtacttt
     4261 tccttatcgg cttcgttgtc cgaattcgcc aattcttcga gcatcgacag cgagcgcttg
     4321 gtcacacctt cacggatcgc cttcacatcg cggctttcct ggaggatttc acgcgacacg
     4381 ttcagcggca aatcagccga atcgaccacc cccttcacga agcgcagata cgcgggcagc
     4441 agttgttcgg cgtcgtccat gatgaacacg cgcttcacgt acagcttcag accgccgcga
     4501 tgatcgcggt tgaacatgtc gaacggcgca tgcgtcggca catacagcag ttgcgtatat
     4561 tcgctgcggc cttcgacgcg gttatgcgtc cacgtcagcg gatcctgatg atcgtgcgag
     4621 agatgctggt agaactgctt gtactgctct tcggtgatgt cgttctttga acgggtccac
     4681 agcgcgctcg cctggttgac ggtctcgtct tcgtccttcg tgaccatctc gctcttttcc
     4741 gcgtcccact cttccttcgc catcaggatg gggagcgcga cgtgatccga gtacttctga
     4801 atgatcgact tcagacgatg cgacgacaac agatcatctt catcggcacg cagatgcagc
     4861 gtgatggtcg tgccgcgcgc ggcgcgctcg atcgtttcga ccgcgaaatc gccctcgccc
     4921 gcgctttccc agcgcacgcc ttccgaggcc ggcaaaccgg cgcggcgcgt ctcgacggtg
     4981 atcctgtccg cgacgatgaa gcccgagtag aagcccacgc cgaactggcc gatcagcgcc
     5041 gcatccttct gctggtcgcc cgacagcttg ccgaagaact ccttggtgcc cgaacgcgcg
     5101 atcgtgccga ggtgcgcgat cgcttcgtca cggctcatgc cgatgccgtt gtcgtcgacc
     5161 gtgacggtgc gcgcggcctt gtcgtacgac acgcgaatcc gcaggttcga atcgttctcg
     5221 tacagtgcgc tgttttcgat tgcctcgaag cgcagcttgt cggccgcgtc ggacgcattc
     5281 gagatcagct cgcgcagaaa gatttccttg ttgctgtaca gcgaatggat catcaattgc
     5341 agaagttgct tcacttccgc ctgaaagctc atggtttctt gtgccatggt cggacttcct
     5401 ctctgttgcg aatgggggat ggtggcggcg aaacgcggtt cgggcttgcc gcgccggatc
     5461 gtcggtgcaa gtggggacgg tccggcgagg tttcaagagg cgcggcgcgc gacgccgtcg
     5521 agatagcggc tcagaaacgc gggcaattcg ggatcgccgc aattggcgat attgaagcgc
     5581 atccacgtgc tcggcgactg ctgcggcgaa aacagcccgc ccggcgtcag cagaaagccg
     5641 gcttcgtgcg cggccgcggc gagcgcgccc gcgtggacgc cgctgtcggc ccacaggaac
     5701 atgccggccg tcggcgtcag gaacagcttc atgccggtct tttcgatcat tcgcacggcc
     5761 ttctcgcgca ccccgtcgag ccgcgcgcgg atgcgctcga cgtgccgccg gtaatggccc
     5821 tcggtcagga tcttgtagat cacgcgctcg ttcaactcgt ggctcgtcat gccgaccag
//
LOCUS       DATASH010000299         5190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6172410,
            whole genome shotgun sequence.
ACCESSION   DATASH010000299 DATASH010000000
VERSION     DATASH010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5190
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6172410"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="VHV99_08420"
     CDS             complement(<1..397)
                     /locus_tag="VHV99_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008342901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX3635498.1"
                     /translation="MRIVAALVIAILATGGGFYASHLMMGGETSQAAEGSTKASGNAV
                     DDLWKAQLQTAAGTQQTLASFKGKPVVVNFWASWCGPCVKEMPTLAALHREYEKKGIT
                     FIGLGIDSEKNVNAFLQKVPVDYPIYIAGF"
     gene            complement(403..1053)
                     /locus_tag="VHV99_08425"
     CDS             complement(403..1053)
                     /locus_tag="VHV99_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--alanine ligase"
                     /protein_id="HEX3635499.1"
                     /translation="MIRKSHFDPLRVREEIAIAAARLIAEDGLDYSTAKRKAARQVVG
                     ETRIGGEWLPDNGQIEDEIREYQAIFQSESQPAVLRRMREVAFEWMQRLQPFNPYLTG
                     AVLNGTAGEHSDIHLQCFCDNPKEVAIYLLNANVQYDVSETRHFAGRGYVETLSFLTR
                     PSRDEEPMGIHIALYDTNDLRGAVRADGRGKLARASAAAVRALLDEPPAPTPATSL"
     gene            1234..2634
                     /gene="mpl"
                     /locus_tag="VHV99_08430"
     CDS             1234..2634
                     /gene="mpl"
                     /locus_tag="VHV99_08430"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007743632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEX3635500.1"
                     /translation="MHIHILGICGTFMGGLAVLAREAGHRVTGCDAGVYPPMSTQLEA
                     QGIELIEGYGVEQIALEPDLYVVGNVVTRGNPLMEIILDRGLPYTSGPQWLGEHVLNG
                     KCVLAVAGTHGKTTTSSMLTWLLEDAGMNPGFLIGGVPLNFGISARLTDSSFFVIEAD
                     EYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFPDLAAIETQFHHLVRTVPGVGRIV
                     TNGREAALERVLTRGCWSGVERFGVQGGWEALPASDGVAVDERFAVYWEGARAGVVDW
                     QVQGEHNRMNALAAIAAARSVGVPPAQATQSLSTFRNVKRRMEVKGSVDGVTVYDDFA
                     HHPTAIETTVAGLRTRIGANSRILAVLEPRSNTMKLGTMKAQLPPSLADADLVFGYGA
                     HEGRDKLGWDLQAALAPLGDKAQAFNDLAMLVKSIVAAARPGDHVLVMSNGGFGGVHQ
                     KLLDALSARPATKERV"
     gene            2631..3218
                     /locus_tag="VHV99_08435"
     CDS             2631..3218
                     /locus_tag="VHV99_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017777077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HEX3635501.1"
                     /translation="MILYLHGFRSSPQSFKARVMRARLEELGLIDEWQCPTLPVSPKE
                     AIALAETLAAQAHDDEITVIGSSLGGFYATWLAEKHGWRAVLLNPAVVPQEDLSCYLG
                     EQPLWHGGGSIVVEPRHIDELRALSVASITRPDRYYLIAATGDEVLDYRTMLAHYPDV
                     RTTLIQGSDHAISDFPSYLDQVLAFCEARPPAVAA"
     gene            3317..>5190
                     /locus_tag="VHV99_08440"
     CDS             3317..>5190
                     /locus_tag="VHV99_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016346263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEX3635502.1"
                     /translation="MNVFFEESGSFKAGAVLSKQNDAFQVELPGGRRAKVRAKDVLIE
                     FEKPNAADLMQQADAIAQDIDLDFLWECAPEEEFPFAELGADYFGDKFGATERAALVL
                     RMHGSPVYFRRKGRGQYQRAPQEQLQMALAGLEKKRQLALVQEGYAEELVAGKLPEAL
                     AGKAIGLLTKPDKNSIEYKALEAAAAARGISQARLMLEVGGIPSARALHEARFLSEFF
                     PHGTGFASVTVPPLPDDLPEADVQAFSIDDVTTTEIDDAFSVEHLADGRVRVGIHIAA
                     PALGISRGDMIDAIARTRLSTVYMPGDKITMLPDSVVEAFTLAEGGLRPALSLYIIVN
                     RETQEIVATETRAERVFVKSNLRHNHLDEHVTEESLAAGSGEYPHKDDIAVLWPLAQA
                     LFEKRQEARAGYGLRREVQRNNDFNFYVEGEHITITPRRRGSPLDLIVAELAILANSS
                     WGAFLNDHSVPGIYRSQRGFGAPGPKRTRMQTTPAPHEGLGVAQYAWSTSPLRRYVDL
                     VNQWQLLACVRHGVTAALVAPFKPKDADLFAVVQGFDDQYSAYADYQRRMEYFWCLRW
                     LKQEDRKHVAASVVKDDLVRLDEIPLMLHVPGLGVHARGTKVMLDVMSLDELTVEASC
                     R"
BASE COUNT          966 a         1513 c         1678 g         1033 t
ORIGIN      
        1 cgaagcctgc aatatagatg gggtaatcga cgggtacctt ctgaagaaac gcgttgacgt
       61 ttttctcgga atcgatgcca aggccaataa acgtaatgcc tttcttctca tattcccgat
      121 gaagtgcagc gagcgtcggc atttccttca cacacgggcc gcaccacgac gcccaaaagt
      181 tcaccaccac gggcttgccc ttgaacgagg ccagcgtctg ctgtgttccg gcggcagttt
      241 gtaactgcgc tttccagaga tcatcgacgg cgttgccgct tgctttcgtg gagccttcgg
      301 ctgcctggct cgtttcaccg cccatcatca ggtggctggc gtagaaaccg ccaccggttg
      361 ccaaaatggc gataacgagg gcggcaacaa tgcgcattga attcataggc tggtggccgg
      421 tgtgggtgcg ggcggctcgt cgagcaatgc gcgtacggcg gcggcgcttg cacgtgcaag
      481 cttgccccgg ccgtccgcgc gaacggcgcc gcgcaggtcg ttggtgtcgt agagcgcgat
      541 gtgaatgccc atgggctctt cgtcgcgcga ggggcgcgtc aggaagctca gggtttcgac
      601 gtaaccgcgg ccggcaaaat gccgggtttc cgatacatcg tattgaacgt tcgcgttcag
      661 caggtaaatg gcgacttcct tggggttatc gcagaagcat tgcaggtgga tgtccgagtg
      721 ttcgcccgct gtgccgttca gcacggcgcc ggtcaggtac gggttgaatg gctggagccg
      781 ttgcatccat tcgaatgcga cttcgcgcat gcggcgcagg acggcgggct ggctttcgct
      841 ctggaagatt gcctggtatt cgcggatttc gtcttcgatc tgaccgttat ccggtagcca
      901 ttcgccgcca atgcgtgttt cgccgacgac ctggcgggcg gctttgcgct tggccgtgga
      961 atagtcgagg ccgtcttcgg cgattagccg cgccgccgcg atggcgattt cttcgcgtac
     1021 gcgcagcgga tcgaaatgtg atttgcgaat catggcggaa tgataccgga gataggtttt
     1081 cgatcacgtt cgggggtggg gccggggtgg cgccgggccg ccgtcggcaa aaatccaagc
     1141 ccatccgtcg gttacaatgt accgctccgg gcgccggaat acctcactca ttccccccgt
     1201 gctaacggcc ctcccgccac gaacacacgc tttatgcaca ttcacatcct cggtatctgc
     1261 ggcacattca tgggtggcct cgcggtcctc gcccgcgaag cgggtcaccg cgtcacgggt
     1321 tgcgacgccg gcgtctatcc gccaatgagc acccagctcg aagcgcaggg catcgagttg
     1381 atcgaagggt atggcgtgga gcaaattgcg cttgaacccg atttgtacgt agtgggcaat
     1441 gtcgttacgc gcggcaaccc gctgatggaa atcatcctcg atcgaggctt gccctacacg
     1501 tccggcccgc aatggctcgg cgaacatgtg ctcaatggca aatgtgtgct cgcggttgcc
     1561 ggcacgcacg gcaaaaccac gaccagttcc atgctgacct ggttgctcga agatgccggc
     1621 atgaatccgg gattcctgat cggcggcgtg cccctgaatt tcggcatctc ggcgcgactg
     1681 acggattcaa gtttcttcgt cattgaagcc gatgaatacg acaccgcctt cttcgataaa
     1741 cgctccaagt tcgttcacta tcgcccgcgc acggccgtac tgaacaacct cgaattcgat
     1801 cacgccgata tcttcccgga tctcgctgct atcgagaccc agtttcatca tctggtgcgt
     1861 accgtgccgg gcgtcgggcg aatcgtgacg aacggccgtg aagccgcgct cgagcgcgtg
     1921 ttgacgcgcg gctgctggtc gggcgtcgag cgcttcggcg tgcaaggcgg ctgggaagcg
     1981 ctgcccgcaa gcgatggcgt tgccgtcgac gagcggttcg ccgtgtactg ggaaggcgcg
     2041 cgcgcgggcg ttgtcgactg gcaagtgcaa ggcgagcaca atcgaatgaa tgcgctggcg
     2101 gcgatcgcgg cggcgcgctc agtaggcgtt ccgcccgcgc aggcgacgca atcgctgagc
     2161 acattccgga acgtgaaacg caggatggaa gtgaagggca gcgtggacgg cgtgaccgtc
     2221 tacgacgact tcgcgcatca ccctaccgcg atcgaaacga cggtagcggg attacggacg
     2281 cgtatcggcg caaacagccg catcctggcg gtcctcgaac cgcgttccaa caccatgaaa
     2341 ctcggcacga tgaaagcgca gttgccgccc agtctcgccg atgccgacct ggtcttcggc
     2401 tacggcgcgc acgaaggccg cgacaaactc ggctgggatc tgcaagcggc actcgcgccg
     2461 ctcggcgata aagcccaggc gttcaacgac ctcgcgatgc tcgtcaagtc aatcgtcgca
     2521 gcggcgcgtc cgggcgatca cgtgctcgta atgagcaacg gcggtttcgg cggcgtgcat
     2581 cagaagctgc tcgatgcact gtccgcgcgg ccggccacca aggagcgggt gtgattcttt
     2641 atctgcacgg ctttcggtca tcgccacaat cgttcaaggc ccgcgtgatg cgcgcgcgcc
     2701 tggaagagct gggactgatc gacgaatggc aatgtcctac cttgccggtc tcgccgaaag
     2761 aagccatcgc tctggctgaa acgctcgcgg cgcaagctca tgacgatgag atcaccgtga
     2821 tcggcagttc gctgggcggg ttttacgcaa cgtggcttgc tgagaaacac ggctggcgcg
     2881 cggtgctgct gaatccggcg gtcgtgccgc aagaggacct gagttgttat ctcggcgagc
     2941 agccgttgtg gcatggcggc ggaagtattg tggtcgagcc gcgtcatatc gacgaattgc
     3001 gtgcgctttc cgttgcatcg atcacccggc cggatcgcta ctatctgatt gcagccaccg
     3061 gcgacgaagt gctcgactac cgcaccatgc tcgcgcatta cccggacgtg cggaccacgc
     3121 tgatccaggg cagcgatcac gcgatcagcg actttcccag ttacctggac caggtgctcg
     3181 cgttctgcga ggctcggccg cccgctgtcg cggcctgaag accctgagcg tttcctttta
     3241 tgttggcttg gcgctttgta cgggacgcga acccgactga acgccacgtc gaccaacagt
     3301 ctttcgagag tattgagtga acgttttttt tgaggaatca ggtagtttca aggccggcgc
     3361 cgttctgtct aaacagaacg acgcgtttca ggtcgagttg ccgggcgggc ggcgcgcgaa
     3421 agtgcgcgcc aaagacgtat tgatcgaatt cgagaaaccg aacgctgccg atctgatgca
     3481 acaggccgat gccattgcac aggacatcga tctcgacttc ctgtgggaat gcgcgccgga
     3541 agaagaattt ccgtttgcgg aactaggcgc ggattatttc ggcgataaat tcggcgcgac
     3601 cgaacgcgct gcgctcgtgc tgcgcatgca cggctcgccg gtgtatttcc gccgcaaggg
     3661 ccgcgggcaa tatcagcgcg cgccgcaaga acagttgcag atggcgttgg ccgggctgga
     3721 aaagaagcgg caactggcgc tggtgcagga gggttatgcc gaggagctgg tcgccgggaa
     3781 gttgcctgag gcgctggccg gaaaggcgat tggtttgctg acgaagccgg ataagaactc
     3841 tatcgaatat aaggcgctcg aagcagcggc cgccgctcgt ggaatttcgc aagcacggct
     3901 gatgctggaa gtgggcggca ttccgtcggc gcgtgcgttg cacgaagcgc ggttcttgtc
     3961 ggaatttttc ccgcacggga cgggcttcgc atcggtcacc gtgccgccgc ttcccgacga
     4021 tctgccggaa gcggacgtgc aggcgttttc tatcgatgac gtcaccacca ctgaaattga
     4081 tgatgcgttc tccgtcgagc atctcgccga tgggcgcgtg cgcgtgggta ttcacatcgc
     4141 ggcaccggcg ctggggatct cgcgcggcga catgatcgat gcaatcgcgc gcacacggtt
     4201 atccaccgtg tacatgcccg gcgacaagat caccatgctg ccggacagtg tcgttgaagc
     4261 cttcacgctg gcggaaggcg gattgcggcc ggcgttgtcg ctgtacatca tcgtgaatcg
     4321 ggaaacgcag gaaatcgtcg cgaccgaaac gcgcgcggag cgtgtgttcg tgaagagcaa
     4381 tcttcgacac aaccatctgg acgagcacgt caccgaggag tcgctcgcgg cgggtagcgg
     4441 cgaatatccg cataaggacg acatcgcggt gctctggccg ctcgcgcagg cgctgttcga
     4501 aaagcgccag gaagcgcgcg cggggtacgg tttgcggcgc gaagtgcagc gcaataacga
     4561 cttcaatttc tacgtggaag gcgagcacat cacaatcacg ccgcgtcgac gcggatcgcc
     4621 acttgatctg atcgtcgccg agctggcgat tctcgcgaat tcctcgtggg gcgcgtttct
     4681 caacgaccac agcgtgccgg ggatttatcg gtcgcaacgc ggatttggcg caccgggccc
     4741 gaagcgcacg cgcatgcaga ccacgcccgc gccgcacgaa gggctgggtg ttgcgcaata
     4801 cgcgtggagt acctcgccgt tgcgtcgata cgtcgacttg gtcaatcagt ggcaattgct
     4861 cgcgtgcgtg cgccatggcg tgacggctgc gctggttgcg ccgttcaagc cgaaagacgc
     4921 cgatctgttc gcagttgttc agggcttcga tgaccagtat tccgcgtacg ccgattatca
     4981 acggcgcatg gagtatttct ggtgcctgcg ctggttgaag caggaggacc ggaagcacgt
     5041 ggcggcgtcg gtggtgaagg acgatctcgt gcgcctcgat gaaatcccgc tgatgctgca
     5101 tgtgccgggt cttggcgtgc acgcgcgcgg gacgaaggtg atgctggacg tcatgagtct
     5161 cgatgaactc acggttgagg cgtcgtgccg
//
LOCUS       DATASH010000300         5184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4195495,
            whole genome shotgun sequence.
ACCESSION   DATASH010000300 DATASH010000000
VERSION     DATASH010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5184
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4195495"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /locus_tag="VHV99_08445"
     CDS             <1..1048
                     /locus_tag="VHV99_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3635503.1"
                     /translation="GFVAGTIASALLSLPDRYDLRRLFIGSAVTAAVATGVLALLPPT
                     GGAVIGLRFLTGMCMAGVYPVGMRLVATWAKADLGLLIGLLVGALTLGSASPHLIAGM
                     PGLDWHLIYLVAAGFAAVAAAAIGFAEVGPNIKRATHIDLRKVAQAWRDPAVRLVNLG
                     YLGHMWELYAMWAWLGLFLQQTLASHGVVDARPHAETLTFGVIAFGALGAWAGGWLAD
                     RVGRTVVTIGAMAVSASCAALIGWLSNAPMPVLIAVAFVWGFSVIADSAQFSASVAEL
                     AEPSSVGTLLTAQTCAGFLLTLVSIHLVPDVVAHLGWKAGLGMLAIGPALGCVAMGRL
                     RAHPDARRLAGGRR"
     gene            1389..2564
                     /locus_tag="VHV99_08450"
     CDS             1389..2564
                     /locus_tag="VHV99_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HEX3635504.1"
                     /translation="MNDIDELLDACKTELHALRRDLHAHPELRFEEHRTAGVVARELE
                     RLGYVVDRGMGGTGVVASLPGKDPSRAIVLRADMDALPIQEANTFSHTSCHRGVMHAC
                     GHDGHTTMLLGAARVLKQLPQLPGTVHFVFQPGEEGGAGARKMIDDGLFERFPAEAVF
                     GMHNWPGLPAGQFGLRVGPIMAAGVRFRIVVTGKGAHAAQPDLGLDPIPLACSVVAQC
                     QTIAARHKDPVVPAVISVCMMRAGDTDNVIPDTAELRGTIRTLSSAVMTELQRQMKQM
                     CDGLAAAYGASIEVEFFQYYPATVNSPAETALCETVIRELFGDASTHANVAPNMTSED
                     FGFMLEARPGTYVLIGNAPHAGTAAGLHHPSYDFNDEIMPAGVRYWVGLAQAYFSRR"
     gene            2648..3418
                     /locus_tag="VHV99_08455"
     CDS             2648..3418
                     /locus_tag="VHV99_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX3635505.1"
                     /translation="MNTLAHPLEPDDATLSCMLSHFTLLEPVFDAMPDIVFFVKDTHA
                     RYALVNRTLASRCGFKEKAALLGKTAEDVFPRRFGRIYTAQDKAIINVGSQMIDQLEL
                     HLYPGRQPGWCLTCKQPLRDAAGKVVGLAGISRDLRADESSHPAYSRLAAVVQSIQEN
                     YVQPLNLKQLAAMADMSVAQLERYFHKVFHLTPRQLLLKTRLDAATALLVSHDKVTDV
                     AALCGYTDHSAFTRQFKATVGVTPTEYRMLLHGTASRS"
     gene            3433..3762
                     /locus_tag="VHV99_08460"
     CDS             3433..3762
                     /locus_tag="VHV99_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635506.1"
                     /translation="MVRRRHHYYVYVVALDDKVWNEARFRRANPDYRFDKPCVYVGMT
                     GLDPDLRFDRHKAGIQANRYVRDYGLRLVPELYEVFNPMPYRGAQDMEVELAIGLREA
                     GYGVWQA"
     gene            complement(3807..4067)
                     /locus_tag="VHV99_08465"
     CDS             complement(3807..4067)
                     /locus_tag="VHV99_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635507.1"
                     /translation="MNQPDHNTSDTPVPAKRPTILRRLLSHHELATLLVLLHAPIDAS
                     AKPEIPQLHEAGLIETVASDAGDSHIRLTSEGNAVLRGLGVK"
     gene            complement(4225..4557)
                     /locus_tag="VHV99_08470"
     CDS             complement(4225..4557)
                     /locus_tag="VHV99_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006052722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635508.1"
                     /translation="MKKFAVVLSVPLLLSACAYFQKNPDAGEPVTDTTTQRSVNDVIA
                     CLTQVATSHNAAFKSTAIPQGQMLDFGDSNIVKVRSDNGATTYRFYAGTRHVGNLWIE
                     AASKTCAP"
     gene            complement(4840..>5184)
                     /locus_tag="VHV99_08475"
     CDS             complement(4840..>5184)
                     /locus_tag="VHV99_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011546893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEX3635509.1"
                     /translation="YHGSGLNHVAFSTGDIFSAIPEFVADGLPVLRIPRNYYEDLAAR
                     YALPDETLEALRAHNILYDRDERGGEFFHAYTEQLDQRFFLEIVERRGGYEGYGAANA
                     AVRLAAQAQRRK"
BASE COUNT          852 a         1547 c         1763 g         1022 t
ORIGIN      
        1 gggctttgtc gcgggcacca tcgcctcggc cttgctctcg ttgccggatc gctacgatct
       61 gcggcgcctg ttcattggct cggcggtcac ggccgccgtc gcgaccggtg tgctggcgtt
      121 gttgccgccc acgggcggtg ccgtcatcgg attgcgtttt ctgaccggga tgtgcatggc
      181 cggcgtttat ccggtcggca tgcggctcgt cgcgacatgg gccaaggccg acctcggcct
      241 gctgatcggc ttgctggtgg gggcgctgac actcggctcg gcaagtccgc atctgatcgc
      301 cggcatgccc gggctcgact ggcatctgat ttatctcgtg gcggcgggat tcgccgccgt
      361 agcggcagcc gcgatcggct tcgcggaggt cggccccaac atcaagcgcg ctacccacat
      421 cgacctgcgc aaggtcgccc aggcatggcg cgaccccgcc gtgcggctcg tcaacctcgg
      481 gtatctcggc cacatgtggg agctctatgc gatgtgggcg tggctcggcc tgttcctgca
      541 gcaaacgctc gcctcgcacg gcgtggtcga tgcgcgtccg cacgcggaaa ccctgacgtt
      601 tggcgtgatt gcgttcggcg cgctcggtgc gtgggccggc ggctggctcg cggaccgcgt
      661 gggacgcacc gtcgtcacca tcggcgcgat ggcggtgagc gcctcatgcg cggcgctgat
      721 cggctggctg tcgaatgcgc ccatgccggt gctgatcgcc gtggcgttcg tgtggggatt
      781 ttcggtcatc gccgactcgg cgcaattctc cgccagcgtg gcggagcttg ccgagccgtc
      841 ttcggtgggc accttgctga cggcacagac gtgtgcggga ttcctgctga cgctcgtgag
      901 tatccatctg gtgccggacg ttgtcgcgca tctcggctgg aaggccgggc tgggcatgct
      961 ggccatcggc ccggcgctcg gctgtgtcgc gatggggcgg ctgcgcgccc atccggacgc
     1021 gcgccggctg gcgggcggca ggcgctagtc gtctcagctt ctctcgtggg tgtcgtcgac
     1081 gaagaagtcg gtggcttcac tgttcacgtt cttgccggat gcgggcgaac gcttcggcga
     1141 tctgatcgcg gcgctcgcgc aggtcagcga cgagttgcga cgagttggtc aaggatctga
     1201 acggtgcacg cgacgctttc cttgcccgca tgctcgaact gggtgtgccg gtggaacgca
     1261 tcgaaaggtt cgatgtgccg gacgcatccg agatcccgcc gttcgcgcga cgtctggcgc
     1321 tcacgggcaa gtatgatgct gtgcttggcg ccgttggggc gcggcgaaca aggtcaatgg
     1381 agatagacgt gaacgatatt gatgaacttc tcgatgcctg caagaccgag ttgcacgcgt
     1441 tgcgccggga cttgcatgcg catccggaat tgcgctttga agagcaccgt acggccggtg
     1501 tggtggcgcg tgagctcgaa cgactaggct atgtggtgga tcggggcatg ggcggcaccg
     1561 gcgtggtggc gagtctgccg ggcaaggacc cgtctcgtgc gatcgttctg cgcgcggaca
     1621 tggatgcctt gccgatccag gaggccaata cgttttcgca tacgtcttgc caccgtgggg
     1681 tgatgcatgc gtgtggacat gacggtcaca cgaccatgct gctcggtgcg gcgcgcgtgc
     1741 tgaagcaact tccgcaattg cccggcacgg tgcactttgt ttttcagcca ggggaagagg
     1801 gcggagcggg cgcacggaaa atgatcgacg acggcttatt cgagcgtttt ccggccgaag
     1861 ccgtgttcgg catgcataac tggcccgggt tgcccgcggg gcagttcggt ctgcgggtgg
     1921 ggccgatcat ggcggcgggg gtgcggttcc ggattgtcgt gacgggcaag ggcgcgcatg
     1981 ccgcgcagcc cgatctgggc ctcgatccga ttccgttggc gtgctcggtc gtggcgcagt
     2041 gccagacgat agcggcgcgt cacaaggatc cggtggtgcc tgcggtgatc tccgtgtgca
     2101 tgatgcgtgc gggcgacacg gacaatgtga ttccggatac tgccgaattg cgcggcacga
     2161 tccgcacgtt gtcctctgcg gtgatgactg agctgcagcg tcaaatgaag cagatgtgcg
     2221 acggtttggc ggcagcctat ggcgcctcga tcgaggtgga gttttttcaa tactatccgg
     2281 cgacggtgaa ttcacccgcc gaaacggcgc tatgcgaaac ggtgattcgc gagttgttcg
     2341 gggatgcctc cacgcatgcg aacgtcgcgc cgaacatgac gtcggaggac ttcggcttca
     2401 tgctggaggc acggccggga acctacgtgc tgattggcaa tgcgccgcac gcgggcacgg
     2461 cggccggctt gcatcacccg tcatatgact tcaatgacga gatcatgccg gccggggtgc
     2521 ggtattgggt cgggttggcg caggcgtatt tttcgcggcg gtgaagacct ggcgcgaacc
     2581 cgaagcggat actgccggca ccggcgtggt cctataatcg agcgcaccga ccacgggaat
     2641 ccgcacgatg aacacgctgg ctcatccgct cgaaccggac gacgcaacgc tgtcctgcat
     2701 gctgtcgcat ttcacgctgc tcgagccggt gttcgacgcg atgccggaca tcgtgttctt
     2761 cgtcaaggac acgcacgcgc gctacgcgct ggtcaatcgc acgctggcct cgcgctgcgg
     2821 cttcaaggaa aaggccgcgc tactcggcaa gaccgccgag gacgtgttcc cgcgccgctt
     2881 cggccgcatt tacacggcac aggacaaagc gatcatcaac gtcggcagcc agatgatcga
     2941 ccaactggag ctgcacctgt atcccggccg tcagccgggc tggtgcctga cctgcaagca
     3001 gccgttgcgc gatgcggcgg gcaaggtggt cgggcttgcc ggcatctcac gcgacctgag
     3061 agcggacgaa agcagccatc cggcgtacag ccggctcgcc gcggtggtgc agtccatcca
     3121 ggaaaactac gtgcagccgt tgaatctgaa gcagctggct gccatggcgg atatgtcggt
     3181 tgcgcaactg gaacgctact ttcacaaagt gtttcatttg acgccgcgcc agctgttgct
     3241 gaaaacacgg ctggatgcgg ccaccgcgct gctggtctcg cacgacaagg tcaccgatgt
     3301 cgccgcgctc tgcggctaca ccgaccacag cgcctttacg cgtcaattca aggcgaccgt
     3361 cggcgtcacg ccgaccgagt accgcatgct gctgcacggc accgcctcgc gtagctaggc
     3421 ggtctgccgc acatggttcg ccgcaggcat cattactacg tctatgtcgt cgcgctcgac
     3481 gacaaggtct ggaacgaggc gcgctttcgc cgcgcgaatc cggattaccg gttcgacaag
     3541 ccttgcgtgt acgtgggaat gacgggattg gacccggatc tgagattcga ccggcacaag
     3601 gccggcattc aggcgaatcg ctatgtgcgc gattacggcc tgcgcctcgt gcccgagctg
     3661 tatgaagtgt tcaatccaat gccttaccgc ggcgcacagg atatggaagt ggaactggca
     3721 attggactac gcgaggcggg gtacggcgta tggcaagcgt aagcggcgtg ccaggtgacg
     3781 cggtgagtga tgcgttaaac gacgcgttat ttcacgccaa gtccgcgcag cacggcattg
     3841 ccttcagatg tcagacgaat atgcgagtcg cctgcgtcgc ttgcgaccgt ttcgatcaag
     3901 ccggcttcat gcaactgcgg aatctcgggt tttgcggagg catcgatcgg tgcgtgcagc
     3961 aataccagca gcgtggccag ctcgtggtgg ctcaagagac gccgcaggat cgtcggccgc
     4021 ttcgccggta ccggcgtgtc gctcgtgttg tgatcgggct ggttcatggg tcgcaccttg
     4081 tgccggaggt tgtgtcggac ggatgatgca gatgaagact taaacgattc ttaagcctca
     4141 ttgtcctgta acaccgtgac acgaagatgg catggacgcc cgttacctac cgttacatct
     4201 cgcccgatca ttcgtctgat tacgttacgg cgcgcaggtc ttgctcgccg cttcgatcca
     4261 cagattgccc acgtgacgcg tgcccgcgta gaagcggtag gtggtcgcgc cgttgtcgct
     4321 gcgcaccttg acgatattcg aatcgccgaa gtcgagcatc tggccttgcg ggatcgcggt
     4381 ggatttgaac gcggcgttgt ggctggtcgc cacttgtgtc aggcaggcga tcacgtcgtt
     4441 gaccgagcgc tgcgtggtgg tgtccgtgac gggctcgccg gcatccggat tcttctggaa
     4501 atacgcgcaa gcgctgagaa ggaggggaac tgacaggact acggcaaact tcttcatatc
     4561 tctcctggcg tttcattcgg ccggcggaac gtgtgttgtc cgcatggggc atgggtgcga
     4621 gccgcacggg ccgtggatca accggcatta tatcggtgcg gcgcggcatg cctgagccag
     4681 tgcagcatgg gctgtgcccg atgtgtggga attgccgagc gcggagcatt gatcgcggag
     4741 gatgtcgcgg ctcgcagtcg cagctcgcag ccgtagccgc agcgcgcgcc gccaccgcag
     4801 cagcttgcag ccacagcccc cagcgtagtg ggccaacccc tacttgcggc gctgggcctg
     4861 cgccgcaagc cgcacagccg cattggccgc gccataccct tcgtaaccac cgcgccgctc
     4921 gacaatttcc aggaaaaagc gctgatccag ttgttcggtg taagcgtgga agaactcgcc
     4981 gccgcgttcg tcgcggtcat acaggatgtt gtgcgcgcgc agcgcctcca gcgtctcgtc
     5041 gggcagcgcg tagcgggcgg ccaggtcctc gtaataatta cgcgggatac gcaacacggg
     5101 cagaccgtcg gcgacgaatt cgggaatcgc gctgaaaatg tcgccggtgc tgaacgcgac
     5161 gtgattcaag cccgagccgt ggta
//
LOCUS       DATASH010000301         7618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2810239,
            whole genome shotgun sequence.
ACCESSION   DATASH010000301 DATASH010000000
VERSION     DATASH010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7618
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2810239"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..273
                     /locus_tag="VHV99_08480"
     CDS             85..273
                     /locus_tag="VHV99_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635510.1"
                     /translation="MSETKETGETKTTKPADIEHPQPAAHSDNEKSKLPERKDEGRNQ
                     SPADHPGKKPGEGEPSVS"
     gene            481..1566
                     /locus_tag="VHV99_08485"
     CDS             481..1566
                     /locus_tag="VHV99_08485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3635511.1"
                     /translation="MFTSPTPSSESHSSPKVSICLPTCNRPELIVECIDSCLAQTYGN
                     LEIVIGDDSKDERTQHLIAERYPNEPRIRYAMNRPSLGQARNVASLFARASGDKILLI
                     HDDDFLTTDGIEKLVSLWKLHPRLEVAFADQYEADHTGAVDFDASTRLNTAFRRTRDA
                     QGLQPLPGRTGLIQMFPNNGWMANAELVKRIGYKKQYGVCCDFVFGTELCLAAHEVFY
                     LHEYVSVYRKTASSISHSTRGTTVAATVSAYGFVQALKLPPQLEPSRKVALRRLVPIV
                     VSVYAKNREPLLGLKVALTHLFAYDYGFSMRLYYHLLMLSRAAVSMRQRRAVAAVVAA
                     APAVIAEPAAAVTERVTDFVTDRSELI"
     gene            complement(1684..2997)
                     /locus_tag="VHV99_08490"
     CDS             complement(1684..2997)
                     /locus_tag="VHV99_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS family transporter"
                     /protein_id="HEX3635512.1"
                     /translation="MTDLSSQTVAAVPDAHDTRRRIFAIVGASSGNLVEWFDFYVYSF
                     CALYFAPAFFPSGNTTTQLLNTAGVFAAGFLMRPIGGWFFGRLADKHGRKTAMMVSVF
                     MMCGGSLVIAVLPTYAQIGALAPALLLVARLFQGLSVGGEYGTSATYMSEVALKGRRG
                     FFASFQYVTLIGGQLCALLVLVILQQTLSTEELKAWGWRVPFVIGALAALVALYLRKS
                     LEETTTAETRQRKEAGTLRGLWLHKGAFATVLGFTAGGSLIFYTFTTYMQKYLVNTAG
                     MHAKTASSVMTAALFVYMLMQPAFGALSDRIGRRRSMLFFGFFATIGTVPLLHALKDV
                     TSPYAAFGLVVVALAIVSFYTSISGLIKAEMFPPEVRALGVGLSYAVANAIFGGSAEY
                     VALWLKSVGNESMFYWYVTVLCAIAGIVSLRMRDPSKEGYLRHEP"
     gene            3077..3265
                     /locus_tag="VHV99_08495"
     CDS             3077..3265
                     /locus_tag="VHV99_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635513.1"
                     /translation="MAIMVRVREFYRIARKDGGLLGSWVFFAWSARFDRLFACGVGLS
                     LISVFGLFLIWLFGLSLN"
     gene            complement(4102..5814)
                     /locus_tag="VHV99_08500"
     CDS             complement(4102..5814)
                     /locus_tag="VHV99_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HEX3635514.1"
                     /translation="MTKHLHNEARGYQPERGKGVPQQQSGHAEAKADDPGAAAAGAVD
                     TAAARGHTGTPTEGQREVRMNVADRRPLPCPSDWTFELIEEYDSHIARVAEQYELDVY
                     PIQLELISAEQMMDAYASVGMPVNYRHWSFGKHFLSTEKSYRRGQMGLAYEIVINSNP
                     CIAYLMEENTMTMQALVIAHAAYGHNSFFKGNYLFRLWTDAHAIIDYLVYAKNYIAEC
                     EERFGLDRVEELLDSCHALMNYGVDRYKRPQKLSLEKEFAARREREAYLQSQVNELWR
                     TLPTRHTPLPEEIEERYPPEPQENLLYFAEKNAPLLEPWEREVIRIVRKVGQYFYPQR
                     QTQVMNEGWATFWHYTLLNTMYAQGKLEDGFMMEFLHSHSNVVYQPPVTKPYYSGINP
                     YALGFSMMSDIRRICEAPTEEDRKWFPELAGSPWLPAMHYAMRNFKDESFVAQYLSPH
                     LIREMRLFSVLDDDMRDALEVSAIHDDSGYQYVRQALSRQYDMHHREPNIQVWAVNTR
                     GDRSLTLRHFMSDNRHLSGDSDEVLKHMARLWQFDVYLESVDENGTVRKRYECKYVPP
                     AVRV"
     gene            complement(5811..7082)
                     /locus_tag="VHV99_08505"
     CDS             complement(5811..7082)
                     /locus_tag="VHV99_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017332328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HEX3635515.1"
                     /translation="MLHQIIDRRLAGKNKSIANRERFLRRVKNYIRRAVSEAVRDRSI
                     KDIQNTQSITIPRKDIAEPSFRHGPGGKREMVHPGNSDYIRGDKIQRPQGGGGGGGGN
                     QASNEGDGQDDFVFELSREEFMQYFFDDLELPRLVKTHLMAVPTWKSIRAGWAAEGTP
                     NNIDVVRSLRSALGRRIALGAPLVNQLHEMERQLEVMKADPDDRREDIKLLEEEIHHL
                     RGRIWRIPFIDPFDLRYVNRVKQPTPSSQAVMFCLMDVSGSMDEQRKDLAKRFFILLY
                     LFLKRNYEKIEVVFIRHHTRAEEVDEDTFFHSTESGGTVVSSALELMQKVMDERYSPT
                     EWNIYGAQASDGDNWTDDSPKCRKILSDDILQKVRYFAYIQVTPEEQNLWLEYAQLAL
                     SQPHMAMKKVETAADIYPVFRELFEKQVASS"
     gene            complement(7310..>7618)
                     /locus_tag="VHV99_08510"
     CDS             complement(7310..>7618)
                     /locus_tag="VHV99_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HEX3635516.1"
                     /translation="PAGISNPKDFRNEIVNFVLRARAANGGKNPAWVSYEKLRVVIEK
                     KMFSNTEELLPVISFNAKGSAEEQRKHEDFVNRMVTKGYTPKQVRLLCDWYLRVRKSS
                     "
BASE COUNT         1564 a         2338 c         2159 g         1557 t
ORIGIN      
        1 ccagccgcag gccgaagcgt ggaaatagcc tcatcgggtc cgtgccttgc tgcgtcatgg
       61 atgttctcaa accaggagca cgccatgagc gaaacgaaag aaaccgggga aaccaaaacc
      121 accaagccgg ccgatatcga acatccccag cccgccgccc acagcgacaa tgagaaaagc
      181 aaactgccgg agcgtaagga tgaagggcgc aatcagtcgc cggcggacca tcccggcaag
      241 aaacccggag agggcgagcc gtcagtcagt tgaaaatata gagtgcccgc cacaagcggc
      301 tttgctgcat ttttgcggca aagccgtata aattttcccc ttccaatacc tttcataacg
      361 cagacatttc ttccgatttt tttacgaatt attttttctt gtaatgcact attttgctgc
      421 atgcagcaaa tgtgtgcgca aagcgaaccg gctgctccct taaccgaaca aggagacgcc
      481 gtgttcactt cccccacgcc ctcctccgaa tcccattctt ctcccaaggt ttccatttgc
      541 ctgccgacct gtaatcggcc tgaactgatc gtggagtgca tcgattcgtg cctcgcgcag
      601 acctacggga atctggagat cgtgatcgga gacgattcaa aagatgagcg cacgcagcac
      661 ttgatcgcag agcgctatcc gaacgagcca cgcatccgct atgcaatgaa ccggccgtcg
      721 ctcggccagg cgcgcaatgt cgcaagcctg ttcgcgcggg cgagcggtga caagatcctg
      781 ctgattcacg atgacgactt tctcacgacc gacggcatcg aaaaactggt gtcgctatgg
      841 aagcttcatc cgcggctaga ggtggcattc gccgatcagt acgaggccga tcacaccggc
      901 gccgtcgact tcgacgccag tacgcgcctg aacaccgcgt ttcggcgcac gagagacgcg
      961 caaggtttgc agccgctgcc gggcagaacc ggcttgatcc agatgttccc caacaacggc
     1021 tggatggcga atgccgaact cgtgaaacgg atcggctaca agaaacagta cggcgtgtgc
     1081 tgcgatttcg tgttcggcac cgaactctgt ctcgccgcgc acgaggtgtt ctatctccac
     1141 gagtacgtct cggtctatcg caagacggcg agttcgatct cgcacagtac gcgcggcacg
     1201 accgtcgcgg cgaccgtcag cgcttacggc tttgtgcaag cactgaaact gccaccgcaa
     1261 ctggaaccgt cacgcaaggt ggcattgcgc cggctcgtgc cgatcgtcgt ttcggtctat
     1321 gcgaagaatc gcgagccgct gctcggcttg aaggtcgcac tcacccatct gttcgcctac
     1381 gactatggtt tcagcatgcg gctctattac cacctgctca tgctctcgcg cgcggccgtg
     1441 agcatgcggc agcgcagagc ggtggctgcg gtggtcgcgg cagcgcctgc tgttattgcg
     1501 gagccggccg ccgctgtgac ggagcgcgtc acggactttg tcaccgatcg atccgaattg
     1561 atctgatgcc gtggcgtggc gtgtcccggc ttgccgaggc gacaaaagaa cggcgaccgg
     1621 gttgcccctg ggcgccgttc tcatgcagcg ctcatgcgcg ccgttcgagc gtgtcgcccg
     1681 tgctcaaggc tcgtgccgca gatacccttc tttcgaagga tcgcgcatgc gcaacgacac
     1741 gatgccggcg atcgcgcaca gcacggtcac gtaccagtag aacatcgact cgttgcccac
     1801 cgacttcagc cacagcgcga cgtattccgc cgagccgccg aagatcgcat tggccaccgc
     1861 gtacgacaat cccacgccca gcgcgcgcac ttccggcggg aacatttcgg ccttgatcag
     1921 accgctgatc gaggtataga agctgacgat cgccagcgcc acgacgacga ggccaaacgc
     1981 cgcatacggg ctcgtcacgt ccttcaaggc gtgcagcaac ggcaccgtgc cgatggtcgc
     2041 gaagaagccg aagaacagca tcgaacgacg gcgaccgatg cggtccgaca aggcgccgaa
     2101 tgccggttgc atcagcatgt agacgaacag cgccgcggtc atcacgctac tggccgtctt
     2161 ggcatgcatg ccggccgtgt tgaccagata cttctgcatg tacgtggtga acgtgtagaa
     2221 aatcagcgac ccaccggccg tgaagccgag cactgtcgca aatgcgccct tgtgcaacca
     2281 caagccgcgc agcgtgccgg cctccttgcg ttggcgtgtt tccgccgtgg tggtttcttc
     2341 gagcgatttc cgcagataca gtgcgacgag cgccgccaat gcgccgatca cgaacggcac
     2401 gcgccaaccc cacgctttca gttcttcggt agagagggtt tgttgcagga tcaccagcac
     2461 cagcagcgcg cacaactgac cgccgatcag cgtgacgtac tggaacgacg cgaagaaacc
     2521 gcggcggccc ttgagcgcga cttcactcat ataggtggcg ctggtgccgt actcaccgcc
     2581 caccgacagg ccctggaaca ggcgcgcgac cagcagcagc gccggcgcca aggcgccgat
     2641 ctgcgcatac gtgggaagca cggcgatcac gagcgagccg ccgcacatca tgaacaccga
     2701 caccatcatc gcggtcttgc gcccgtgctt gtcggcgagg cgtccgaaga accagccgcc
     2761 gatcggacgc atgaggaagc cggcggcgaa gacgcctgcg gtattgagca actgcgtggt
     2821 ggtgttgccg ctcgggaaga aggccggcgc gaagtacagc gcgcagaatg agtacacgta
     2881 gaagtcgaac cactcgacca ggttgcccga tgaagcacca acgatcgcga agatgcggcg
     2941 ccgggtgtcg tgggcgtcgg gaaccgctgc cacggtttgg ctggataggt cggtcatgct
     3001 cttgtctttt ccttgtcttt aaacggaggt ggcggtcgtg gcgccgcctt cacgacacgc
     3061 atatcgaggt gccgcggtgg caatcatggt gcgcgtgcgc gaattttaca gaattgcaag
     3121 gaaagatggg ggtttgttag gttcgtgggt gttttttgcc tggtcggcgc gttttgatcg
     3181 tttgtttgcc tgcggcgttg gcctttcctt gatctcggtc tttggccttt tcttgatctg
     3241 gctttttggc ctttccttga attgatagtg gtctattagc gtcgcccctg tgcggggcag
     3301 cacctacttt tctttgccgg ccgcaaagaa aagataggca aaagaaagcg gctcaaaccg
     3361 ccagctcata agcgggtccc ccgcacagtc gcggtagtgg tgcatctgga atctgtgttc
     3421 tcgcacattt cgcgtgagtg accaggcagt catacttccc gcctcgcact gcgtgctcgc
     3481 cggaagggtt catcatgcaa accagtggct tcgtttggcg cggcgggggc catcggcttc
     3541 gcctcggcga tctgccacgg cgaaagagat gcaggaggtc aggaagagag acaatacata
     3601 tcgcgctgca ccactacgcc gaatgaggcg tgagcgcgaa aacacagcag atggttttaa
     3661 gaagtgccct tcggcgcgcg cagcgccgcc ggaagtatga cggccttgtc actcacgcgg
     3721 agtgcgcgag aacacagatt ccaaatgcac cactaccgcg actgtgcggc ggacccgctt
     3781 aagaatttgc ggtgtgagct gctttctctt gcctacttct ctttgcagcc ggcaaagaga
     3841 agtaggtgcc gccccgcaca ggggcaacgc taatagacca ctaagaattc aaggaaaggc
     3901 caaaaagtca gtgcaaggaa agcccaacgc cgcaggcaaa caaccaaacc caacgccgca
     3961 ggcaaacaat tcaaggaaag cccaccgcaa taaaagcaca gaaaaaaaaa cgccgcgcag
     4021 gccaacacct tgtggaaatc gcaaaaaaac caccccctgc ggagcaccca aacccggccc
     4081 cgcaggggaa aaaaccaatc gtcaaaccct caccgccggc ggcacatact tacactcata
     4141 ccgcttccta accgtgccgt tctcatcaac gctttccaga tagacatcga actgccacaa
     4201 ccgcgccatg tgcttgagca cctcatcgct atcaccggaa agatgccggt tatcgctcat
     4261 gaaatgccgc agcgtcaggc tccggtcgcc gcgcgtgttc accgcccaca cctgaatatt
     4321 cggctcccga tgatgcatgt cgtactgccg cgacaaagcc tgcctcacgt actgataccc
     4381 gctgtcgtca tgaatcgccg agacctccag cgcatcgcgc atatcatcat ccagcaccga
     4441 gaacagacgc atttcccgga tcagatgcgg cgacagatac tgcgcgacga aactctcgtc
     4501 cttgaaattg cgcatcgcat agtgcatcgc cggtaaccac gggctgccgg ccagttccgg
     4561 gaaccacttg cggtcctctt cagtaggcgc ctcgcagatc cggcgtatat cgctcatcat
     4621 cgagaaaccg agcgcatacg gattgatgcc gctgtagtac ggtttcgtga cgggtggctg
     4681 gtagaccaca ttgctgtgcg aatggagaaa ctccatcatg aagccgtctt ccagcttgcc
     4741 ttgggcgtac atcgtgttga gcaaggtgta gtgccagaac gtcgcccagc cttcgttcat
     4801 cacctgggtc tgccgctgcg gataaaaata ctgcccgacc ttgcgcacga tgcggatcac
     4861 ctcccgctcc cacggttcga gcagcggcgc gttcttttcg gcgaaataca gcagattctc
     4921 ctgcggctca ggcggatagc gctcctcgat ttcctcgggc aacggcgtgt gccgggtcgg
     4981 caaggtgcgc cacagttcgt ttacctgcga ttgcagataa gcctcacgct cgcggcgcgc
     5041 cgcgaactcc ttttccagcg atagcttctg cggacgtttg taacggtcca cgccgtaatt
     5101 catcaacgcg tggcaggagt cgagcagttc ctccacccgg tcgagtccga agcgctcctc
     5161 gcactccgcg atgtaatttt tcgcgtagac aaggtagtcg atgatcgcgt gcgcatccgt
     5221 ccacagcctg aacagatagt tgcccttaaa gaacgagttg tggccataag ccgcgtgcgc
     5281 gatgacgagc gcctgcatcg tcatcgtgtt ctcttccatc agatacgcaa tgcaagggtt
     5341 cgaattgatg acgatctcat acgccagccc catctgcccg cgacggtagc tcttttcggt
     5401 ggagagaaag tgtttgccga acgaccagtg acggtagttc accggcatgc cgacggacgc
     5461 gtaagcgtcc atcatctgtt cggcgctaat gagttcgagc tggatcggat acacatcgag
     5521 ttcatattgc tctgcaacac gggcaatatg cgagtcgtac tcttcgatca attcgaaggt
     5581 ccagtcggac gggcacggca aaggccgtct atcggctacg ttcatacgga cttccctttg
     5641 cccctcagtg ggcgttccgg tgtgtccccg tgctgcagcg gtgtcgactg ctccggctgc
     5701 ggcggctccc ggatcgtccg ccttcgcctc ggcatgcccc gactgttgct gcggcacgcc
     5761 tttcccacgc tccggctgat agccgcgcgc ttcgttgtgc aggtgttttg tcatgaagac
     5821 gccacctgtt tttcaaacaa ctcgcgaaac accggataga tgtcggccgc ggtttcgacc
     5881 ttcttcatcg ccatatgcgg ctggctcagc gcgagttgcg catattcgag ccacaagttc
     5941 tgctcttccg gcgtgacctg gatatatgca aaatagcgca ccttctgcag gatgtcatcc
     6001 gaaagtatct tgcggcactt gggtgaatcg tcggtccaat tgtcgccgtc ggacgcctga
     6061 gcgccgtaaa tattccattc agtgggcgaa tagcgctcgt ccatcacctt ctgcatcaac
     6121 tcgagcgcgc tcgacacgac cgtgccaccg ctttccgtcg aatggaagaa cgtgtcttcg
     6181 tcgacttctt cggcgcgcgt atggtggcgg atgaacacta cctcgatctt ttcgtagtta
     6241 cgcttgagga acagatacag cagaatgaaa aaacgtttgg ccagatcctt gcgctgctcg
     6301 tccatggaac ccgacacatc catcagacag aacatcacgg cctggctcga tggcgtgggc
     6361 tgcttcaccc ggttcacgta gcgcagatcg aacggatcga taaatggaat ccgccagatg
     6421 cgcccacgca aatggtggat ttcctcttcg agcagtttga tatcttcgcg acggtcgtcg
     6481 ggatcggcct tcattacctc gagttgccgt tccatctcgt gcaactggtt gacgagcggc
     6541 gcaccgagcg cgatgcggcg gccgagcgcg ctgcgcaacg agcgaaccac gtcgatattg
     6601 ttcggtgtgc cttccgccgc ccagccggcg cggatacttt tccacgtggg cacggccatc
     6661 agatgggttt tgacgagacg cggcagttcg aggtcgtcga agaaatactg catgaattct
     6721 tcgcggctca gctcgaacac gaagtcgtcc tgaccgtcgc cctcgttgct ggcctgattg
     6781 cccccgccgc ctccgccgcc tccttgcggg cgctggatct tgtcgccgcg gatatagtcg
     6841 gaattgcccg gatgcaccat ttcgcgcttg ccgccgggtc catgccggaa cgacggttcc
     6901 gcgatgtcct tgcgcgggat ggtgatgctc tgggtgttct gtatatcttt gatgctgcgg
     6961 tcgcgaaccg cttccgagac ggcgcgacga atgtagtttt tgacgcggcg caaaaagcgc
     7021 tcgcgattcg caatgctctt gttcttccca gccaacctgc ggtcgatgat ttgatgaagc
     7081 acgcccggtc tcccgtgtcg accagagtta gcgctttagc gcttactctg gtcccatgca
     7141 ccgcatttcg atcggagttg gcgctttagc gcttcctcca acccccatgc agttgcgctg
     7201 atttccggtg cgcgagacgc atgttgcgaa cacaaacgct atgtccatgc agcttgcgcc
     7261 tcgcaaggtg cccgcgcgga cggaccgcgc gggcggcacg cagtgcgtat catgacgact
     7321 tacgcacgcg cagataccag tcacacaaca agcgcacctg cttcggcgta tagcccttcg
     7381 tgaccatgcg gttgacgaag tcttcgtgct tgcgttgctc ttccgccgag cccttggcgt
     7441 tgaacgagat caccggcaac agttcctccg tgttcgagaa catcttcttt tcgatcacga
     7501 cgcgcagctt ctcgtagctg acccaggcgg gattcttccc tccattggca gcacgcgcac
     7561 gcagcacgaa gttcacgatc tcgttgcgga agtccttcgg attgctgatg cccgcggg
//
LOCUS       DATASH010000302         4496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4592091,
            whole genome shotgun sequence.
ACCESSION   DATASH010000302 DATASH010000000
VERSION     DATASH010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4496
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4592091"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..951
                     /locus_tag="VHV99_08515"
     CDS             79..951
                     /locus_tag="VHV99_08515"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX3635517.1"
                     /translation="MLKPDIDSLVPHVPFNRRTFIKAVLGTGFAAAVLPVSAQTIHTD
                     SDGLEAGEVAVRSGDTLVPAYRAQPKGKTHLPVIIVIHEAFGVHEHIADVCRRFAKLG
                     YLAIAPDLFVREGDPMALPTIQQISNQILDKVPDEQALSDLDATVAWAGEHGGDLDRL
                     GVNGFCWGGRIAWLYAEHNPRLKAVAAWYGRVGGDHSATTPANPLDRVADLHAPVLGL
                     YGLQDQSIPQASLEQMKQAIAQGPQAGRGSQFVVYDDAGHAFFADYRPSYKKADAEDG
                     WRRVLAWFKQHGVA"
     gene            complement(1104..1972)
                     /locus_tag="VHV99_08520"
                     /pseudo
     CDS             complement(1104..1972)
                     /locus_tag="VHV99_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004529026.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
     gene            complement(2184..3290)
                     /locus_tag="VHV99_08525"
     CDS             complement(2184..3290)
                     /locus_tag="VHV99_08525"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006027818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX3635518.1"
                     /translation="MSNLSNALQLRSVHSQLPVTAYFDEALLTREIDTLFKKGPRYIG
                     HDLMVPEAGNYFALPSESEGRVLVRNQQSQVELLSNVCRHRQAIMLNGRGQTENIVCP
                     LHRWTYDLNGQLLGAPHFADNPCLNLGATPLQNWQGLLFEAQGRDVARDLARLGTKQH
                     FDFSGFMFDHVEVHECNYNWKTFIEVYLEDYHVAPFHPGLGSFVNCDDLTWEFGEWYS
                     VQTVGVHKDLEQPGSPTYRKWHDEVLRFRGGNPPEFGAIWMVYYPGIMIEWYPHVLVV
                     SWLIPRGPQKTTNVVEFYYPEEIALFEREFVEAERAAYMETAREDDEIAERMDAGRRA
                     LMERGESQVGPYQSPMEDGMQHFHEFLRRELGAI"
     gene            3578..3886
                     /locus_tag="VHV99_08530"
     CDS             3578..3886
                     /locus_tag="VHV99_08530"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004190549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEX3635519.1"
                     /translation="MAKTASKDTAAAPAEGVDTTPLPENYEAAQAELEGLVARMESGN
                     LSLEESLGAYRRGAALVAFCQQQLEKVEQQVRVLDGETLKPLPMNSAGTAATESGDDL
                     "
     gene            3883..>4496
                     /locus_tag="VHV99_08535"
     CDS             3883..>4496
                     /locus_tag="VHV99_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX3635520.1"
                     /translation="MTFEQWTRSVLERVEAALEHYLPTEATEPAKLHEAMRYAVLGGG
                     KRVRPLLCHAAGELTGARAECLDAAAAALEMIHVYSLVHDDMPCMDDDALRRGKPTVH
                     VEYDEATALLVGDALQSQAFVALTSDVLAPAQQASLVRELALASGSIGMCGGQAIDLA
                     SVGHTLTRAQLETMHRMKTGALLRAAVRMGALAGEPPGADAMRSL"
BASE COUNT          825 a         1363 c         1460 g          848 t
ORIGIN      
        1 gctcgagaag gttgcatgtg caacaggatg cgggtgaagc gtatcctgtc gcctcaacct
       61 acattcgagg agcgccgcat gctgaaacct gacatcgaca gcctggtccc gcacgttcca
      121 ttcaatcgac gtaccttcat caaagccgtg ctcggcaccg gtttcgccgc ggccgtgcta
      181 ccggtgtcgg cgcaaaccat ccataccgat agcgacggcc ttgaagccgg tgaggtcgcc
      241 gtgcgttcgg gcgacacgct cgtgccggcg tatcgcgcgc agcccaaggg taagacgcat
      301 ctgccggtga tcatcgtgat ccacgaggcg ttcggcgtgc atgagcatat tgccgacgtg
      361 tgccggcgct ttgccaagct cggttatctg gcgatcgcgc cggatctgtt cgtccgcgag
      421 ggcgatccca tggcgctgcc gaccatccag cagatcagca accagattct cgataaggtg
      481 ccggacgagc aggcgctgag cgatctcgac gccaccgttg cgtgggcggg cgagcacggc
      541 ggcgatctgg accggctggg cgtgaatggc ttctgctggg gaggacgcat cgcttggctg
      601 tatgccgagc acaatccccg gctgaaggcg gtggcggcat ggtacggccg ggtcggcggc
      661 gatcacagtg cgacgacgcc ggcgaacccg ctcgatcgcg tggctgatct gcatgcgccg
      721 gtgctggggc tgtatggctt gcaggatcag agcattcccc aagcctcgct cgagcagatg
      781 aagcaggcca tcgcgcaggg tccgcaggca gggcgcggtt cgcaattcgt cgtatatgac
      841 gacgcgggcc acgcgttttt cgcggactac cgtccgagtt ataaaaaagc cgatgccgaa
      901 gatggctggc gccgtgtgct ggcatggttc aagcagcatg gggtggcttg agtcattccc
      961 gtgcctggcc gggcgtgttt cgatcgtcgt tgacgaacca taagggtgtg ccgacgccct
     1021 cggttggatg aatggagaaa ggccgtggct gctttttcag cggccacggc cttttcgcta
     1081 atcgtaccgc tccacgtatc ggcttaagga ttcgaccccg tcgcaatcgg ccgcttcgga
     1141 tcagaactcc attcgctcca cgatcccgca tacagcgccg cgccatgcat gccggcgatc
     1201 tccatcgcga gcgcgttcac acatgccgtc acgcccgagc cgcattgcag cacgacgtgt
     1261 tccggcggcg tgtcgcaagc aacgcgtgca attcctcgcg cagcgtgtga gcgggtttgt
     1321 agcggccgtc ggcggtgagg ttgtccttga agaatcgatt gagcgcgcca ggaatatggc
     1381 cgccgacgcg atccaacgtt tcgttctcgc cgcgatagcg atcgttcgcg cgcgcgtcca
     1441 ccacgacgtg ctccttggtg ccgagattgc gttcgacggt ctgcgcgtcg accgtgacgg
     1501 acagcggcgc gccggctttg aagtcaccgg tgttctgcgc cgggacgtct tgcgtcaacg
     1561 ggtgaccggc ggcctgccag gcttgcaagc caccgtcgag tagcgcaacc gcgtcatggc
     1621 cgagccagcg cagcaaccac catgcgcgcg cggcgtacat gccgccctgc gcgtcataag
     1681 cgaccacctg ttgaccctgc ttaagccccc gcgactccag cgtctccacc agccgcgcac
     1741 gatccggcag cggatggcgg ccattgtggc cggtcttcgg tcctgacaga tcgcgatcga
     1801 ggttcagata atgtgcgccc ggcagatgcc cggccagata ggctttctcg cccgcctcgg
     1861 tgtcggccag atcgaaacgg caatcgatga cgaacacgct gcccggcgcg gcggccagcc
     1921 gctccgcgag gtttgtcgcg gagatgagtg tggtgtagtg agtgtgcggc atgatgtctc
     1981 ctcgatacgg gcgattgcgg ctccggtccg gtggcctggc gcggcgggcg gatggccggg
     2041 cgatattgtt agtctaaaca aaaaaagacg ggccgaagcc cgtctttccc tgctttgctg
     2101 cggctcgtgc acaactcacg cagggatcgg ctcaaacgcc gatggatgcg gagcatgaag
     2161 ggagcacgcg cggaccgcgc ggatcagatc gcgccgagtt cgcgccgcaa gaactcgtga
     2221 aagtgctgca tgccgtcttc catcggactc tgatacgggc cgacctgaga ttcgccgcgt
     2281 tccatcagcg cgcgacggcc agcgtccatg cgctcggcga tctcgtcgtc ttcgcgggcc
     2341 gtttccatgt aggcggcgcg ctcggcttcg acgaactcgc gttcgaacag cgcgatttcc
     2401 tcagggtaat agaactcaac cacattggtg gttttctgcg gaccgcgcgg aatcaaccac
     2461 gacacgacca gcacgtgcgg ataccactcg atcatgatgc cggggtagta caccatccag
     2521 atcgcgccga actcgggcgg attgccgccg cggaaacgca gcacttcgtc gtgccatttg
     2581 cggtaggttg ggctgcccgg ctgctccaga tccttgtgaa cgccgaccgt ctgcacgctg
     2641 taccactcgc cgaattccca cgtcagatca tcgcaattga cgaagctgcc gaggcccgga
     2701 tggaatggcg cgacgtggta gtcctccaga tagacctcga taaaggtctt ccagttgtag
     2761 ttgcactcgt gcacctcgac gtgatcgaac atgaagcccg aaaaatcgaa gtgttgtttg
     2821 gtgcccagcc ttgccagatc gcgcgcgacg tcgcgcccct gcgcttcgaa cagcagacct
     2881 tgccagttct gcagcggcgt ggcgccaaga ttcaggcagg ggttatccgc aaaatgaggt
     2941 gcaccgagga gctggccatt caggtcgtac gtccagcgat gcagggggca gacgatgttc
     3001 tccgtctggc cgcggccgtt gagcatgatg gcttgccggt ggcggcacac gttcgacagc
     3061 agttcgactt gcgactgctg gttacggacg agcacgcgcc cttcgctctc gctcggcaaa
     3121 gcaaaataat ttcccgcttc gggcaccatg agatcgtggc cgatgtagcg aggacctttc
     3181 ttgaaaaggg tgtcgatttc gcgcgttagg agcgcttcgt caaagtaagc cgtgactggc
     3241 agctggctgt gaacagaccg caactgcaat gcattgctca gattggacat tcccactccc
     3301 gatgaagacg tgaaagcagt gaacaaccca accatcgaag attcgattta gggagcccgc
     3361 gattataccc gtttcccctt ccttggggcg tttaagtgcc tgatttgggt caaaaatgtc
     3421 aaggaagttc acgtttttcg ccgcagaccg atcgattttt ttctgcgaga ctgctagtgc
     3481 ggcgcgcagg gcaaaggcgg ggtgtaggtt ggtgagcgga tcggaaatgt ctcttttgcc
     3541 gtagaatgtc cgacttgttt taattttgcg actattcatg gcgaagaccg cgtcgaaaga
     3601 tactgctgct gcgcctgctg aaggcgtcga taccacgccg ttgcccgaga actacgaggc
     3661 cgcccaggcg gagctggaag ggctggtcgc gcgcatggaa agcggcaatc tcagcctcga
     3721 ggagtcactg ggcgcctacc ggcgcggtgc ggcgctcgtg gcgttttgcc agcagcagct
     3781 cgaaaaggtc gagcagcagg tgcgcgttct cgacggcgag acgctcaagc cgctgcctat
     3841 gaatagcgca ggcacagccg ctactgaaag cggggacgac ctatgacatt cgaacaatgg
     3901 acacgctcgg tgctcgagcg cgtcgaagcg gcactggaac actacctgcc cacggaagcg
     3961 accgagccgg ccaaactgca tgaggccatg cgctacgcgg tgctgggcgg cggcaagcgg
     4021 gtgcgcccgc tgctgtgcca cgcggccggc gagctgaccg gcgcgcgcgc cgagtgcctg
     4081 gatgcggcag cagcggcgct ggaaatgatc cacgtgtatt cgctcgtgca cgacgacatg
     4141 ccgtgcatgg acgacgacgc gttgcgtcgc ggcaagccta cggtacacgt cgaatatgac
     4201 gaagctacgg cattgctggt gggcgacgcg ctgcaatcgc aggcgttcgt cgcgctgacg
     4261 tcggacgtgc tggcgccggc gcaacaggcg tcgctcgtac gcgaactggc cctggcgagc
     4321 ggctcgatcg gcatgtgcgg cggtcaggcc atcgacctgg cgagtgtcgg ccatacgctg
     4381 acgcgcgcac aactggaaac catgcaccgg atgaaaaccg gcgccttgct gcgtgccgcg
     4441 gtgcgcatgg gcgcgctggc gggcgaaccg ccgggtgcgg acgccatgcg ttcgct
//
LOCUS       DATASH010000303         3023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_239438,
            whole genome shotgun sequence.
ACCESSION   DATASH010000303 DATASH010000000
VERSION     DATASH010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239438"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1517)
                     /gene="priA"
                     /locus_tag="VHV99_08540"
     CDS             complement(<1..1517)
                     /gene="priA"
                     /locus_tag="VHV99_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007581634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX3635521.1"
                     /translation="MSVFVRVALDHPLATLFDYRYDLAPTPLPGMLVRIPFGRRDAVG
                     LICEVTDHTDVPVDKLRDVAAICSTLAPLADEWRALTLFAAEYYQRGLGEVALPALPQ
                     VLRDASRWAKLLAPEERYRLMPEGRASLPDALPPRAIALRRLADALAANPTLPAAEAR
                     ALHPKARVMLDEWQTRGWISLETGDGPEHQIALDPALEKAALHATDVLLPQLTAEQSD
                     AVDAITGAKGFAAFLLHGVTGSGKTEVYLHALAHLLGSRPDAQALVLVPEINLTPQFE
                     AAFRTRFAALPEGAIVTMHSGLAEGERARNWLAAHTGRARLVLGTRLAILASLPKLAL
                     IVVDEEHDPAYKQQEGLRYSARDLAVYRANKLEIPVVLGSATPSLESWWQAGQGRYTK
                     LTLTRRAVATAVLPSVRLIDLEEERQRGRASVEGLSGPLIAAMKTRLERGEQSLVFLN
                     RRGYAPILSCDACGWVAGCPRCSAYVVLHKPERALRCHHCGWESRIPKSCPDCGNV"
     gene            complement(1979..>3023)
                     /gene="hemE"
                     /locus_tag="VHV99_08545"
     CDS             complement(1979..>3023)
                     /gene="hemE"
                     /locus_tag="VHV99_08545"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010107210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEX3635522.1"
                     /translation="RQPTDYTPIWLMRQAGRYLPEYNATRSRAGSFMGLATNPDYATE
                     VTLQPLERFPLDAAILFSDILTIPDAMGLGLNFVQGEGPRFERTVRTEEDVAKLHVPD
                     IDATLRYVTDAVSQIRRALTDAQGRQKVPLIGFSGSPWTLACYMVEGGGSPDHHTVKA
                     MAYTRPDLMHRILDINAQAVVAYLNAQIEAGAQAVMIFDTWGGALADGAYQRFSLDYI
                     AKVVAGLKREHDGVKVPVITFTKGGGLWLEQIAAIGVDAVGLDWTMNLRQAREKVGDR
                     VALQGNIDPSVLFASPAVIRTEARAVLDSFGNFPGHVFNLGHGISQFTHPENVAELID
                     EVHRHSRAIRAKS"
BASE COUNT          584 a          929 c          936 g          574 t
ORIGIN      
        1 acattgccgc agtccgggca ggatttggga atccgcgact cccagccgca atggtggcaa
       61 cgcagcgcgc gctcgggctt gtgaagcacg acgtaggcgc tgcatcgcgg gcagccggcg
      121 acccagccgc aggcgtcgca cgaaaggatg ggtgcgtaac cccgacggtt gaggaacacg
      181 aggctttgct cgccgcgctc caggcgggtt ttcatcgcgg cgatcaacgg ccccgacaat
      241 ccctcgaccg acgcccgtcc gcgctgccgc tcctcttcga ggtcaatcag ccggacgctc
      301 ggaagaactg ctgtcgccac ggcgcgccgc gtgagcgtga gcttggtgta tcggccctgc
      361 ccggcttgcc accagctttc gagcgatggc gtcgccgacc ccagcacaac cggaatctcc
      421 agcttgttcg cccgataaac cgccagatca cgcgccgaat agcgcaagcc ttcctgctgc
      481 ttgtaggcgg ggtcgtgttc ctcatcgacc acaatcagcg ccagcttcgg caacgacgcc
      541 aggattgcca gccgcgttcc cagcacgagc cgcgcgcggc ccgtgtgcgc cgcaagccag
      601 ttgcgggcgc gttcgccttc ggccagcccg ctatgcatcg tcacgatggc gccctccggc
      661 aacgcggcga aacgcgtgcg gaaagcggct tcgaattgcg gcgtgaggtt gatttcaggc
      721 acgagcacga gcgcttgcgc gtcgggacgc gaaccgagca aatgggccag tgcgtggaga
      781 taaacctccg ttttgccgct gccggttacg ccgtgcagaa gaaacgcggc gaaacctttc
      841 gcgccagtga tggcatcgac ggcatcggac tgctcggcag tcaattgcgg caaaagcacg
      901 tcggttgcgt gcagcgcggc cttttcaagc gcgggatcga gcgcgatctg gtgctccggc
      961 ccgtcgccgg tttccaacga tatccatccg cgagtctgcc attcatcgag catcacccgc
     1021 gcctttggat gcagcgcgcg agcctcggcg gcaggtaagg tgggattggc ggcgagggca
     1081 tcggcgagac ggcgcagcgc gatggcccgt ggcggcaacg catcgggcaa actcgcgcgc
     1141 ccttcgggca tcagccgata ccgctcttcc ggcgcgagca atttcgccca gcgcgaggca
     1201 tcgcgcagaa cttgcggcaa cgcgggcagc gccacttcgc ccagaccgcg ctggtaatac
     1261 tcggccgcga atagtgtcag cgcgcgccat tcatcggcta gtggcgcgag cgtgctgcaa
     1321 atggcggcca catcgcgcaa cttgtctacg ggcacgtcgg tatgatccgt gacctcacaa
     1381 atcaggccaa ccgcgtcgcg acgtccgaaa ggaatccgta ccagcatgcc gggcagcggc
     1441 gtgggggcca gatcgtatcg atagtcgaaa agcgtcgcca gagggtggtc cagcgcgacc
     1501 cggacaaaga cgctcaattg acgcacccgc cgcaagccgc gctggcgcaa tcgttccacc
     1561 tgaacatagg gctgtgcggg gcgtagttaa agtgaaacat cacaatcgac gctaacctgc
     1621 ggattatttt tgagatttcg aagctttcca caggcctgtg gataactttg ttgagaactt
     1681 tgattgacag ggacaaaaag tggcgatctc gcttgttgtg tgcggtagcc cacatttcgg
     1741 gttgttctca aatttctctt attaatcatg tatttgaaga caccaaacat gtcttcataa
     1801 ggggccttag gtatgatttg aagatggcgc gccgcaacgt acaaaatgtg cataagtcaa
     1861 ctcttgacat tggcggaacg gccaatctac gggcgcttcg cggcactttt gtctaaccgc
     1921 ccaaacgttt gacgtaagct tttcgtgacg catagcagca atcttttttg accaaacgtc
     1981 agctctttgc gcgaatggcg cggctatgcc gatgcacttc atcaatgagt tccgcgacgt
     2041 tttccggatg cgtgaactgc gaaataccgt ggccgaggtt gaaaacatgg ccggggaaat
     2101 tgccgaagct atcgagcacg gctcgcgcct cggtgcgaat cacggccggc gacgcaaaca
     2161 gcacagatgg atcgatgttg ccctggagcg ccacccgatc gcccactttt tcgcgcgcct
     2221 ggcgcaggtt catggtccag tccagaccga ctgcgtccac gccaatagcc gcgatctgtt
     2281 ccagccacaa accaccgccc ttcgtgaacg tgatgaccgg cactttgacg ccgtcgtgct
     2341 cgcgctttag tcctgcgacg accttcgcga tgtagtcgag cgagaagcgc tgatacgcgc
     2401 cgtccgccag tgcgccaccc caggtatcga agatcattac ggcttgcgcg cctgcttcga
     2461 tctgcgcgtt cagataagca acgaccgctt gcgcgttgat atcgagaatg cgatgcatca
     2521 ggtccggccg cgtgtaggcc atggccttga ccgtatggtg atcgggcgat ccgccgcctt
     2581 cgaccatgta gcacgcgagc gtccaggggc tgcctgagaa gccgatcagc ggcactttct
     2641 ggcggccttg tgcgtcggtt agcgcgcggc ggatttgcga taccgcatcg gtcacatagc
     2701 gcagcgtggc gtcgatatcc ggcacatgca gcttcgcaac atcctcttcg gtgcggaccg
     2761 tgcgttcgaa tcggggacct tcgccctgca cgaaattcag gccgaggccc attgcgtcgg
     2821 ggatggtcag gatgtccgag aacaggatgg cggcatcgag cggaaagcgt tccagcggct
     2881 gaagcgtgac ttccgttgcg taatcgggat tcgttgcgag acccatgaag ctgccggcgc
     2941 gcgaacgcgt ggcgttgtat tcgggcagat agcggcccgc ctgacgcatc agccagatcg
     3001 gcgtgtagtc ggtcggctgg cgc
//
LOCUS       DATASH010000304         3454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1822570,
            whole genome shotgun sequence.
ACCESSION   DATASH010000304 DATASH010000000
VERSION     DATASH010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1822570"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..1250)
                     /locus_tag="VHV99_08550"
     CDS             complement(72..1250)
                     /locus_tag="VHV99_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX3635523.1"
                     /translation="MTHPLDDVLVVSIEQAIAAPLASRHLADWGARVIKIERPESGDF
                     ARHYDTVMDGISSQFVWVNRSKESLAIDIKTEFGQETLESLLARADVFIQNLAPGAAR
                     RQGLDAKTLSERFPQLIACDISGYGADGPYSQKKAYDLLVQCETGFLSINGSEDSPAK
                     CGLSIVDIATGMYALNGILMGLHQRARTGKGMAFEVSLFDAMTEWMSYPAYYTRGAGR
                     PLSRTGPRHATIAPYGPFTAGDGGSVFFGIQNEREWQRFCQIVLEDPAMAVDTRFVTN
                     SLRLANRAVLETMITTHFAPLSSEQVIARLDRASIANGRMNDVDGLLNHPQVLERKRY
                     RAVQTESGPHDLFLPAITIPGVEPVMRPVPALGEHTARILAELGETSAAAGHVQGARS
                     "
     gene            1658..2416
                     /locus_tag="VHV99_08555"
     CDS             1658..2416
                     /locus_tag="VHV99_08555"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006403979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HEX3635524.1"
                     /translation="MTNETKVALVTGAASGIGEATAQLLASRGYRVAIADLRDDPAAA
                     AAERIRSNGFEAMPVKVDVADEASVKAMVETVVSRWGRLDVLVNSAGLESARPFLEIP
                     LAEFRKVMDVNTMGTWLCCQQAIAQMLRQQSGSIVNLSSIAGQKGGGMLGTAAYATSK
                     GAVIALTKSLAREFARSGIRVNAVAPAFTLTDFVRRQLDTKPPGFIDTIYAATPMGRG
                     AQPAEIAAVVAFLASDEASFVTGHVYNADGGSAI"
     gene            complement(2413..3297)
                     /locus_tag="VHV99_08560"
     CDS             complement(2413..3297)
                     /locus_tag="VHV99_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012366934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX3635525.1"
                     /translation="MPRPSLNALRAFEATARLRSFGDAAEELSVTHGAISRHIRSLED
                     TLGVLLLLRNAHSTEPTPEGARLAEGLSRAFNLIQASVEQVRPGPLTLSCSASIMMYW
                     LLPRLGKLHRKHASLEVGFNTGSGEIDFTRENISVAIRLNTIKAPKDIEPKPLIDEWI
                     GPVCSPEYLRATMIKAPDDLNRARLLATNTRPGAWQDWFDSAQLAALQPAIMESFEHF
                     YLLIQAAKCGLGIANVPRMLVRDELDSGALVAPFGFVPGPRKLVLWVAPHLEARPQTA
                     ALVDWLGAELRDTEGVPA"
BASE COUNT          629 a         1085 c         1087 g          653 t
ORIGIN      
        1 ccagcggtgc ccgggcactg cgagcacgga tcgcatcggc cgcacggttt tcagatccgc
       61 gtgtgcgccc gtcacgaccg tgccccctgc acgtgcccgg ctgctgcgct tgtctcaccc
      121 agttccgcga gaatccgcgc cgtatgctcg ccgagcgcgg gcacgggccg catgaccggc
      181 tcgacgcccg gaatcgtgat ggccggtaga aacagatcat gcggaccgct ctccgtctga
      241 accgcgcgat accgcttgcg ctccagcact tgcggatgat tcagcaggcc gtccacatcg
      301 ttcatgcgcc cattggcgat ggacgcccga tcgagccttg cgatcacctg ttcgctcgac
      361 aagggcgcga agtgcgtggt gatcatcgtt tccagcaccg cgcgattggc aagacgcagc
      421 gagttcgtga cgaagcgcgt gtcgacggcc atcgcgggat cctccagcac gatctggcaa
      481 aaacgctgcc actcgcgctc gttctggatt ccgaagaaga cgctgccgcc gtcgccggct
      541 gtaaacggtc cgtacggcgc aatcgtcgca tgacgcggcc cggtgcgcga caacggccgg
      601 ccggcgccgc gggtgtaata cgcgggatag ctcatccact cggtcatcgc atcgaacagc
      661 gagacttcga acgccatgcc tttgccggtg cgcgcgcgtt gatgcaatcc catcaggatg
      721 ccattgagcg catacatgcc ggtggcgata tcgacgatgg acaagccgca cttcgccggc
      781 gaatcctcgc tgccattgat cgacagaaag ccggtctcgc actgcaccag caggtcgtac
      841 gccttcttct gactgtacgg gccgtctgcc ccgtagccgg agatatcgca ggcgatcagt
      901 tgcgggaaac gttcgctcag tgtttttgcg tcgagcccct ggcggcgcgc ggcgcccggt
      961 gcgaggttct ggatgaaaac gtccgcgcgt gcgagcaacg attccagcgt ctcctgcccg
     1021 aattcggtct tgatgtcgat cgcgagactt tccttcgagc ggttcaccca gacgaactga
     1081 ctggagatgc cgtccatcac cgtgtcgtaa tgccgggcaa aatcgccgga ctcgggacgt
     1141 tcgatcttga tgacccgcgc gccccagtcg gccagatgac ggctcgcgag cggcgcggcg
     1201 atggcctgtt cgatcgaaac gacgaggacg tcgtcgagag gatgagtcat gaatgcgtcc
     1261 cgaagaagga gtggaaatgg atcggctacg cgtacgaggc gcgtcagtgc ataccggtgc
     1321 tcgatgcggt ggctggtcca gtgcttgaaa gccgaatgta ctgaatcatg gatgcaaaga
     1381 ggaaatactg tttttgccta cccgttatag gacggaccta tggcacaagc gccgtggtgt
     1441 gcctgtcatc ggcagcaatg gttgccgcag tgcacgcgtg aacaaaggtg atgcaacacc
     1501 gtacccggtg cctcgtttaa agtgagtttt cgtcgcaggc gacagaaatc atttcgtttg
     1561 atctgacctg ggctgcggcc gtatattttt agccgacctc ctccggcttg ctgccggacg
     1621 gccactcatc cacgcagatc cacgcatgct cagaaaaatg acgaacgaga caaaagttgc
     1681 tctggtcacc ggtgccgcca gcggcattgg tgaagctacc gcacagttgc tggcgagtcg
     1741 cggctaccgc gtcgccatcg ccgatctgcg cgacgacccg gctgccgccg cggccgaacg
     1801 aatccgctcg aacggcttcg aagcaatgcc cgtcaaggtc gacgtcgccg acgaagcgag
     1861 cgtcaaggcc atggtcgaaa cggtcgtgtc ccgttggggc cggctcgacg tgctggtcaa
     1921 tagcgcggga ctcgaaagcg cgcggccctt tctcgagatt ccccttgccg aattccgcaa
     1981 ggtgatggac gtgaacacga tgggcacctg gctgtgctgc cagcaggcga ttgcgcagat
     2041 gctcaggcag cagagcggct cgatcgtcaa tctgagttcg attgccggtc agaagggtgg
     2101 cggcatgctc ggcaccgcgg cgtatgcgac ctctaaaggc gcggtgattg cgttgaccaa
     2161 gtcgctcgcc cgcgagttcg ccaggtccgg tatccgcgtc aacgcggtgg ccccggcctt
     2221 cacgctgacg gacttcgtgc ggcgccagct cgacaccaag ccgccaggct tcatcgatac
     2281 gatctatgcg gccacgccga tggggcgcgg cgcgcagccc gccgaaattg cggccgtcgt
     2341 ggcgttcctc gcctcggacg aagcctcgtt cgtgaccggc cacgtctaca acgccgatgg
     2401 cggctcggcg atctaggccg gcacaccttc cgtatcgcgc agttccgcgc cgagccagtc
     2461 gaccagcgcc gccgtttgcg ggcgcgcctc gaggtgcggc gcgacccaca acacgagctt
     2521 gcgagggccc ggcacgaacc cgaacggggc gacgagcgcg cccgagtcga gctcgtcacg
     2581 caccagcatg cgcggcacgt tcgcgatgcc cagtccgcat ttggccgcct gaatcagcag
     2641 atagaagtgt tcgaacgatt ccatgatggc gggttgcagc gccgccagct gcgccgaatc
     2701 gaaccagtcc tgccacgcgc ccggccgcgt gttggtggcg aggaggcgcg cgcggttcag
     2761 gtcgtccggc gcttttatca tcgtggcgcg cagatattcg ggcgaacaga ccgggccgat
     2821 ccattcgtcg atcagcggct tcggctcgat gtccttgggc gccttgatcg tattcaggcg
     2881 gatcgcgacg ctgatgtttt cccgcgtgaa atcgatctct cctgaaccgg tgttgaagcc
     2941 cacttcaaga gacgcatgct tgcgatgcaa tttcccgagc cgcggcagca gccagtacat
     3001 catgatcgac gccgaacacg acagtgtgag cggtccgggc cgcacctgct cgacgctcgc
     3061 ctgaatcaga ttgaaggccc gcgagaggcc ttcggcaagc cgcgcgcctt cgggggtggg
     3121 ttcggtggaa tgcgcgttgc gcaacagcag caatacgccg agcgtgtctt cgagtgaacg
     3181 gatatgccgg ctgatcgcgc cgtgagtcac cgacaactct tccgccgcgt cgccgaaact
     3241 gcgtaagcgc gccgtggcct cgaaagcccg cagtgcgttg agtgatggtc gtggcacggg
     3301 ttgttcctat tgccggaaag cgctccggcg tcgtctcgct attgtgagtt ttttgcagca
     3361 cctccggaaa tcatatcgtt tgatggtttt tccggtcaat cttatattcc gacatgccac
     3421 gaaggtattc gtcgcgttct cacgcacaat gagc
//
LOCUS       DATASH010000305         3509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5183960,
            whole genome shotgun sequence.
ACCESSION   DATASH010000305 DATASH010000000
VERSION     DATASH010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5183960"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="VHV99_08565"
     CDS             complement(<1..520)
                     /locus_tag="VHV99_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010117629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX3635526.1"
                     /translation="MPFAAVNGIQLFYRIDATAGSDAPWLVLSNSLGADVSMWTPQVE
                     TLAAKYRVLRYDTRGHGRSDAPKGPYTLAQLVGDVIGLLDTLGIKRAHYCGLSMGGLT
                     GIALAARHPERFERVVLSNTAALIGSDAIWTPRAAKAREEGGMPALADAVLARWFTAG
                     FMTAQPLLLSGIR"
     gene            complement(520..1905)
                     /locus_tag="VHV99_08570"
     CDS             complement(520..1905)
                     /locus_tag="VHV99_08570"
                     /EC_number="5.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047472 - 3-carboxy-cis,cis-muconate
                     cycloisomerase activity [Evidence IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxy-cis,cis-muconate cycloisomerase"
                     /protein_id="HEX3635527.1"
                     /translation="MSEPTFSATGRLTDLICGTREMNAVWSARQTVQSMLDVEAALAR
                     ASAEHGVIPAAAVDAIVAACNADLIDADALMAGAASGGNLAIPLVKQLTAAVKSQDAD
                     AAKYVHWGATSQDIIDTGTVLQLRSTFDLLEAGLHEACDALALQAQIYRTTPAIGRTW
                     LQQALPITLGLKFAQWLDALTRHRERLAQCRLRVLALQFGGAAGTLASLRDKAPLVAQ
                     SLANDLGLQLPSLPWHTQRDRVAEAAACFGMLTGTLGKIARDISLQMQTEVGELAEPA
                     AAGKGGSSTMPHKRNPVGCASVLTAAMRAPNLVATVFSGMVQEHERALGGWQAEWDAL
                     PDLARLTGGALAQISAIVSNIEVNAGRLTANLDLTHGLILGEAVMLALGDRIGRLDAH
                     TLVERASKQAVATGATLYDVLSNDERVTQHLSLVQLQALLDPVNYVGQAHAFVDAALL
                     LHRVPSSRSFS"
     gene            complement(1905..3107)
                     /gene="pcaF"
                     /locus_tag="VHV99_08575"
     CDS             complement(1905..3107)
                     /gene="pcaF"
                     /locus_tag="VHV99_08575"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HEX3635528.1"
                     /translation="MKEAFICDAIRTPVGRYGGALSPVRADDLGAVPLRALMERNKDV
                     DWNAVDDLIFGCANQAGEDNRNVARMSALLAGLPDSVPGSTINRLCGSGMDAIGVAER
                     AIKSGEAALMIAGGVESMSRAPFVQGKATTAFSRQADIFDTTIGWRFINPLMKKLHGV
                     DSMPETAENVATDYKVSREDQDAFAVRSQQKASRAQKDGTLAQEIVPVSIAQKKGDPV
                     LVSQDEHPRETSMEALARLKGVVRPDGTVTAGNASGVNDGAAALLLADEDSARRFGLV
                     PRARVLGIATAGVPPRVMGIGPAPASQKLMKRLGMTIDQFDVIELNEAFASQGLAVLR
                     MLGIADDDPRVNPNGGAIALGHPLGMSGARLVTTATYQLHRTGGRFALCTMCIGVGQG
                     IAIAIERV"
     gene            complement(3132..>3509)
                     /locus_tag="VHV99_08580"
     CDS             complement(3132..>3509)
                     /locus_tag="VHV99_08580"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX3635529.1"
                     /translation="LDICVLGAFQVSAKGDLANWHTGAPDAIPAVGGAMDLAIGAKKV
                     YVMMELLTKQGESKLVAECSYPVTGVNCVNRVYTDHGVFDVTPNGFVVREIIEGLSFD
                     ELQKLAQVPLTFEPLDAAATLEA"
BASE COUNT          672 a         1184 c         1035 g          618 t
ORIGIN      
        1 cgcgaatacc ggacaacagc agcggctgcg cggtcatgaa gccagccgtg aaccagcgcg
       61 ccaggacggc atcggccaat gccggcatgc caccctcctc gcgggctttc gccgcgcgcg
      121 gcgtccagat ggcgtccgag ccgatcagcg ccgccgtatt cgacaacacc acgcgctcga
      181 aacgctccgg gtggcgtgcc gcgagcgcaa tgccggtcag cccgcccatc gaaaggccgc
      241 aatagtgcgc ccgcttgatg ccgagtgtgt cgagcaaacc gattacgtct cccacaagtt
      301 gggcgagcgt gtacggaccc ttgggcgcgt ccgaacggcc atgaccgcgc gtgtcataac
      361 gcaacacgcg atatttcgcc gcgagcgttt cgacttgcgg cgtccacatc gagacatcgg
      421 cgccgagcga attcgacaac acgagccagg gtgcgtcgct gccggcggtg gcatcgatgc
      481 gataaaagag ttgaataccg ttgacggcgg caaaaggcat tacgaaaaac tcctggaaga
      541 agggactcga tgcaaaagca gcgcggcatc gacaaaagcg tgcgcctggc ccacgtagtt
      601 caccgggtcg agcaaggctt gcagttgaac aagagagagg tgttgcgtga cgcgttcgtc
      661 gttcgagagc acgtcgtaaa gcgttgcgcc ggtcgccacg gcttgtttcg atgcacgttc
      721 cacgagcgtg tgcgcgtcga gcctgccaat ccggtcaccc agcgcgagca tcaccgcttc
      781 gccgagaatc agtccgtgcg tgagatccag gttggctgtg agccgcccgg cattcacttc
      841 gatgttcgag acaatcgccg agatctgcgc gagtgcgccg cccgtcaggc gggcaagatc
      901 tggcagcgca tcccattcgg cctgccagcc gccgagcgcg cgttcatgtt cctgaaccat
      961 gccgctgaat acagtcgcga ccagattcgg cgcgcgcatg gcggccgtta aaacagaagc
     1021 gcagcccacg ggattacgct tgtgcggcat cgtcgatgaa ccgcccttgc cagccgcagc
     1081 cggctctgcc agttcgccga cttctgtctg catctgcagg gagatatcgc gggcgatttt
     1141 tcccagcgta ccggtcagca tgccgaaaca ggccgctgct tccgctacgc gatcgcgttg
     1201 ggtgtgccac ggcaacgaag gaagttgcag gccaaggtca ttggcaagcg attgggcgac
     1261 caacggcgcc ttgtccctca agctcgccaa cgtgcccgcc gcgccgccga attgcagcgc
     1321 cagcacccgc aaacgacact gcgcaagacg ctcgcgatgc cgtgtcagcg catccagcca
     1381 ttgggcgaat ttaagaccga gcgtaatcgg caacgcctgc tgaagccagg tccggccgat
     1441 tgccggcgtc gtccgatata tctgcgcctg aagtgcgagc gcgtcgcaag cctcatgcag
     1501 acccgcttcc agtaagtcga acgtggaacg cagttgcaag acagtaccgg tatcgataat
     1561 gtcctggctc gtcgcgcccc aatgcacgta cttggcggcg tcggcgtcct ggcttttaac
     1621 agcagctgtc agttgcttca ccagcggaat cgcgagattg ccgccggatg cggcgccggc
     1681 catcaacgcg tcagcatcaa tcagatcagc gttgcaagcg gccacgatcg cgtcaaccgc
     1741 ggccgccggg atcacaccat gctcagccga tgccctcgca agcgccgcct cgacatccag
     1801 catcgactgg acggtctggc gcgcggacca cacggcgttc atctcgcgtg tgccgcagat
     1861 caggtccgtc agccgtcccg tcgccgaaaa tgtcggctca gacatcacac acgctcgatg
     1921 gcgatcgcaa taccctgacc cacgccaata cacatcgtgc agagcgcaaa gcgtccgccg
     1981 gtccgatgca actgatacgt cgcggtcgtc accaggcgtg cgccgctcat gccgagcgga
     2041 tggccgagcg cgatcgcgcc gccgttcgga ttgacgcgag gatcgtcgtc ggcaataccg
     2101 agcatgcgca gcacagccaa accttgcgat gcaaacgcct cgttcagctc gatcacatcg
     2161 aattgatcga tggtcatgcc caggcgcttc atcagctttt gcgacgccgg cgccggaccg
     2221 atgcccatca cgcgcggcgg cacgccggcg gttgcaatgc ccaacacgcg cgcacgcggc
     2281 acgaggccga aacgcctggc gctgtcttca tcggcgagga gcaaggccgc cgcgccatcg
     2341 ttcacgcccg acgcattgcc cgccgtcacc gtgccatccg gacgcacgac gcccttcagt
     2401 ctggcgagcg cttccatgct cgtttcacgc ggatgttcgt cttgcgagac cagcaccgga
     2461 tcgccctttt tctgcgcgat cgacaccggc acgatctctt gtgcaagcgt gccgtccttc
     2521 tgagcgcgcg acgctttctg ctggctgcgc acagcaaacg cgtcctgatc ttcgcggctc
     2581 accttatagt cggtcgcgac gttttcggcc gtctccggca tcgaatccac gccatgcagt
     2641 ttcttcatca acggattgat gaagcgccag ccgatggtcg tatcgaagat atctgcctga
     2701 cgcgaaaacg ccgtggtcgc cttgccctgc acgaacggcg cacggctcat gctttccacg
     2761 ccgcccgcga tcatcagcgc ggcttcaccc gacttgatcg cccgctcggc cacgccgatt
     2821 gcatccatgc ccgatccgca caagcggttg atggtcgagc ccggcacgct gtcgggcaaa
     2881 ccggccagca acgccgacat gcgcgcgacg ttgcgattgt cttcgcccgc ctggttcgcg
     2941 cagccgaaga tcaggtcgtc gacggcgttc cagtccacgt ccttattgcg ctccatcaac
     3001 gcgcgcagcg gcacggcgcc aaggtcgtcg gcacgaaccg gggacaaagc gccgccgtag
     3061 cggccgaccg gcgtgcgaat cgcgtcgcaa ataaaagctt ctttcatgca cttctcctga
     3121 ttacttcctg atcacgcttc gagcgtggcg gccgcgtcaa gcggttcaaa cgtcagcggc
     3181 acttgcgcca gtttctgcag ttcgtcgaac gacaggcctt cgataatttc ccgcaccacg
     3241 aacccgttcg gcgtcacgtc gaacacaccg tgatccgtgt acacgcgatt cacgcaattc
     3301 acgcccgtca ccggatagga acattcggcc accagcttgc tttcgccctg cttggtgagc
     3361 agttccatca tcacgtagac cttcttcgcg ccgatcgcga gatccatcgc gccgccgaca
     3421 gcgggaatgg catcgggcgc gccggtatgc cagttggcga ggtcaccctt cgcggacacc
     3481 tggaacgcgc cgagcacgca gatatcgag
//
LOCUS       DATASH010000306         9998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1427393,
            whole genome shotgun sequence.
ACCESSION   DATASH010000306 DATASH010000000
VERSION     DATASH010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 9998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9998
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1427393"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="VHV99_08585"
     CDS             <1..428
                     /locus_tag="VHV99_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006496735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEX3635530.1"
                     /translation="LEHPEPRIFLGLALRGIAHAALDLSDGLAGDLLHILERSDMQAT
                     VNVDAVPRSTALRRLSPEIQRRCALAGGDDYELCFTAPVAARAKVEAAGREVAVPVTR
                     IGTISGLQTAADRPAIGWRDAAGSPLTLTLQGFDHFHAD"
     gene            418..990
                     /locus_tag="VHV99_08590"
     CDS             418..990
                     /locus_tag="VHV99_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEX3635531.1"
                     /translation="MQTDPPLVPADDLIGNPPPNASGPRAPRPQPRRATARFMLSHPL
                     HILSLGFGSGLSPIAPGTIGTLFAWASFAVLSRYLTVVEWGVLIVAGFFGGIAICGFT
                     AKKLGIDDPSSIVWDEIIAFWLVLLIVTPVTLSGQFWAFIVFRFFDMVKPPPIGYLDR
                     RLKGGFGIMFDDLVAAFFTLLVIALWRMSI"
     gene            1094..1594
                     /locus_tag="VHV99_08595"
     CDS             1094..1594
                     /locus_tag="VHV99_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEX3635532.1"
                     /translation="MPTDSVVHQLAIRVSNRLRDERLMLVTAESCTGGMVATAITDIS
                     GSSGWFERGFVTYSNQAKSEMIGVPADLIDKHGAVSEQVARAMAEGALRNSRAQVSLS
                     ITGVAGPGGGTETKPVGMVSFGWSNRLHTSVETRVFKGDREKIRVQAAAHALRGVLAL
                     LDEREY"
     gene            1626..2147
                     /locus_tag="VHV99_08600"
     CDS             1626..2147
                     /locus_tag="VHV99_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX3635533.1"
                     /translation="MPNVSASHTAKDELIHRFDLKPHPEGGFFSETYRSAEPVRRDDG
                     SMQTRSASTAIYYLLCDGAHSAWHRIKSDEVWHFYAGEPLNVHVLDQAGALITHRLGN
                     ALTHSDAVFQAVVPAGLWFAAECADPATFALVGCTVAPGFEFSEFELADVEPLKAQHP
                     QHAALIERLGPGT"
     gene            complement(2363..3178)
                     /gene="pyrF"
                     /locus_tag="VHV99_08605"
     CDS             complement(2363..3178)
                     /gene="pyrF"
                     /locus_tag="VHV99_08605"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX3635534.1"
                     /translation="MSNFIQTLNDAWQRTNSLLCVGLDPEPSKFPGALANRPEAIFDF
                     CRTIVDATAPYASSFKPQIAYFAAHRAEDQLEQLIAHIHAHHPGLPVILDAKRGDIGS
                     TAEQYAREAFERYQADAVTVNPYMGFDSIEPYLEHAGKGVIVLCRTSNPGGSDLQFLE
                     AGGRPLYQVVAQLAADKWNASGQLGLVVGATFPKEIEVVRGIVGDMPLLIPGIGAQGG
                     DVQATVNAGRTANGTGMLINSSRAIIYAGKDEDFAEAAAKVAMETRDRINAFR"
     gene            complement(3397..4449)
                     /locus_tag="VHV99_08610"
     CDS             complement(3397..4449)
                     /locus_tag="VHV99_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HEX3635535.1"
                     /translation="MTVANLVSASSQTRRARLNAAANPVLPGPSTSAVAVGETSGGDV
                     ACITLANPHLRLDVAPMLGGGVTRFDWRNDGALTPIFRRCRHVGSDTQPNQLACYPLL
                     PYSNRIGGGHFNVAGRRVEVPQNRADEPLPIHGDGWLSAWQVAQSNRENVRLTLDRRH
                     GKPYAFRATQTYTLDGATLVITLEIENAGREALPFGLGVHPFFVRDADTELSAAAGGL
                     WLSGDDWLPVRHVPTPPAWQFGVAYPLPEAIVNHAFTGWSGQAAVVWPKRRLSLNIAA
                     DTDYYVLYTPPGEDFFCFEPVDHPINAMNLAGGACENGMTMLGRGERLTRTFRFTVER
                     TGLRSMPGARGARRKQ"
     gene            complement(4661..5611)
                     /locus_tag="VHV99_08615"
     CDS             complement(4661..5611)
                     /locus_tag="VHV99_08615"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX3635536.1"
                     /translation="MSADTRVGLPAGSPPPCAALLLDTKCTLGEGATWCAKTSRFYWT
                     DIEGARLWRYDPSDGSTSWPMPERLATFALCADPTHLLLGLATHLAFFNLVTGETQHI
                     VDVEAGMNTRVNDGRCDRQGRFVFGTKDEGSPLQAVGAFYRLNHDLSLERLPLPAPAI
                     SNSIAFSPDGATMYYCDSPTREIRTCDYCADGSIVNDRVFTTLTDASGEPDGSTVDNE
                     GGLWNAQWGGRRVVRYGPDGVETERVEVPTAQPSCVAFGGPPNGLPSGQAELNTLYIT
                     SARIDLDAAALAGDPGAGGVFIATPARHGLPEPVFQGSPR"
     gene            complement(5608..6408)
                     /locus_tag="VHV99_08620"
     CDS             complement(5608..6408)
                     /locus_tag="VHV99_08620"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3635537.1"
                     /translation="MSSPANANDRLADNAFARYPSLVDRTVLITGGATGIGASFVEHF
                     AAQGARVAFFDIDATAGEALADELGDSKHKPLFVPCDLTDVDALQKAIADVKAALGPI
                     QVLVNNAANDKRHTIAEVTRESFDAGIAVNIRHQFFAAQAVIEDMKAANAGSIINLGS
                     ISWMLKNGGYPVYVMSKSAVQGLTRGLARDLGHFNIRVNTLVPGWVMTEKQKRLWLDD
                     AGRRSIKEGQCIDAELQPADLARMALFLAADDSKMITAQDLIVDGGWA"
     gene            complement(6449..7465)
                     /gene="araH"
                     /locus_tag="VHV99_08625"
     CDS             complement(6449..7465)
                     /gene="araH"
                     /locus_tag="VHV99_08625"
                     /EC_number="7.5.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006025224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose ABC transporter permease AraH"
                     /protein_id="HEX3635538.1"
                     /translation="MQARENLAQQAAKGAAEALIPQSNDKAKWWQQITEYSLILIFIV
                     MFVTMSLTVDHFFSIENMLGLALSISQIGMVACTMMFCLASRDFDLSVGSTVAFAGVL
                     CAMVLNATGNTFIAIIAAVAAGAVIGFVNGAVIAYLRINALITTLATMEIVRGLGFIV
                     SHGQAVGVSSDTFIALGGLSFFGVSLPIWVTLLCFIVFGVMLNQTVYGRNTLAIGGNP
                     EASRLAGINVERTRVFIFLIQGAVTALAGVILASRITSGQPNAAEGFELNVISACVLG
                     GVSLLGGRATISGVVIGVLIMGTVENVMNLMNIDAFYQYLVRGAILLAAVLLDQLKNR
                     GSRD"
     gene            complement(7520..9061)
                     /gene="araG"
                     /locus_tag="VHV99_08630"
     CDS             complement(7520..9061)
                     /gene="araG"
                     /locus_tag="VHV99_08630"
                     /EC_number="7.5.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015612 - ABC-type L-arabinose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042882 - L-arabinose transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HEX3635539.1"
                     /translation="MSATLRFDNIGKVFPGVRALDGVSFDVNVGQVHGLMGENGAGKS
                     TLLKILGGEYQPDSGRVMIDGNEVRFTSAASSIAAGIAVIHQELQYVPDLTVAENLLL
                     GQLPNSLGWVNKREAKRFVRERLEAMGVGLDPNAKLRKLSIAQRQMVEICKALLRNAR
                     VIALDEPTSSLSHRETEVLFKLVRDLRADNRAMIYISHRMDEIYELCDACTIFRDGRK
                     IASHPTLEGVSRDTIVSEMVGREISDIYNYSARPLGDVRFAAKAIEGHALVQPASFEV
                     RRGEIVGFFGLVGAGRSELMHLVYGADHKKGGEIVLDGKPIKVRSAGEAIRHGIVLCP
                     EDRKEEGIVAMATVSENINISCRRHYLRAGMFLDRKKEAETADRFIKLLKIKTPSRRQ
                     KIRFLSGGNQQKAILSRWLAEPDLKVVILDEPTRGIDVGAKHEIYNVIYQLAERGCAI
                     VMISSELPEVLGVSDRIIVMRQGRISGELARQDATEQTVLSLALPQSSTALPGTASAA
                     TEQAA"
     gene            complement(9341..>9998)
                     /locus_tag="VHV99_08635"
     CDS             complement(9341..>9998)
                     /locus_tag="VHV99_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434364.1"
                     /note="with AraGH is involved in the transport of
                     arabinose into the cell; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX3635540.1"
                     /translation="DDRLVDGAGKPIASVPHMGISAYNIGKQVGDGLAAEIKKRGWDM
                     KDVGAIDVTYEQLPTAHDRTSGATDALIAAGFPKANIIAAPQAKTDTENAFNAANIAL
                     TKNPNFKHWVAYGLNDEAVLGAVRAAEGRGFKADNMIGIGIGGSDSALNEFKKPSPTG
                     FYGTVIISPKRHGEETSTLMYDWITQGKEPPMLTLTTGMLATRDNVADVRQKMGLAAN
                     "
BASE COUNT         1760 a         3272 c         3070 g         1896 t
ORIGIN      
        1 ccctcgagca tccagagccg cgcatcttcc tcggcctcgc gttgcgaggc atcgcgcatg
       61 cggcgctcga tctctcggac ggcctcgccg gggatctgct gcacatcctc gaacgctcgg
      121 acatgcaggc cacggtcaat gtcgacgccg tcccgcgctc caccgccctg cgccgcctct
      181 cgcccgagat ccagcgacgc tgcgcgctcg ccggcggcga cgattacgag ctgtgcttca
      241 ccgcgccggt tgccgcgcgc gcgaaggtcg aagcagccgg ccgcgaagtg gcggtaccgg
      301 ttacgcgcat cggtacaata agcggtcttc aaacggcggc cgaccgcccg gcgatcggct
      361 ggcgcgacgc cgccggctcg ccgctcactt tgacgttgca aggcttcgac cacttccatg
      421 cagactgatc ccccgctggt ccccgccgac gacctcatcg gcaacccgcc gcccaacgcg
      481 tccggtcccc gcgcaccgcg tccgcagccg cgccgcgcta ccgcgcgttt catgctgtcg
      541 catccgctgc acattctgtc gctgggtttt ggcagcggct tgtcgccgat cgcgccgggc
      601 acgatcggca cgctgttcgc atgggcatcg ttcgccgtct tgagccgtta cctgaccgtg
      661 gtcgagtggg gcgtcctgat cgtcgccggg tttttcggcg gcattgcgat ttgcggcttc
      721 acggcgaaga agctgggtat cgacgatccg tcgtcgatcg tctgggacga aatcatcgcc
      781 ttctggctgg tgctgctgat cgttacgccg gtcacgctga gcgggcagtt ctgggcgttt
      841 atcgtgttcc gcttcttcga catggtgaaa ccgccgccga tcggctattt ggatcgccgg
      901 ctgaaaggtg gctttggcat catgttcgat gacctggtcg ccgcgttttt cacgctgctc
      961 gtgatcgcgc tctggcgcat gtcgatttaa ccgctgcccg ttggctggtg ccggtcggcc
     1021 actttccgcc ggccaccagc caccgctcgc cggccgcctc ccgcagccat tccccgtttt
     1081 ccggattgac gccatgccta ccgattccgt ggttcatcag cttgcgattc gcgtgagcaa
     1141 ccgcctgcgc gacgagcgcc tcatgctcgt caccgccgaa tcctgcactg gcggcatggt
     1201 ggcgaccgcg attaccgata tttccggcag cagcggctgg ttcgagcgcg gcttcgtcac
     1261 gtattcgaat caggcgaagt ccgaaatgat cggcgtgccg gccgacttga tcgacaagca
     1321 cggcgcggtc agcgagcaag tggcgcgcgc gatggccgag ggcgcgctgc gcaacagccg
     1381 cgcgcaggtg tcgctatcga ttaccggcgt cgccggcccg ggcggcggca ctgagaccaa
     1441 gccggtcggc atggtgtcgt tcggttggag caatcggctg catacgtcgg tggaaaccag
     1501 ggttttcaag ggcgaccgcg agaagatccg cgtgcaggcc gcagcgcacg cgttgcgcgg
     1561 cgtgctggcc ctgctcgacg agcgcgaata ttaacttccg gttcaacagc cgaggcgccg
     1621 ccccgatgcc caacgtatct gcttcgcaca ccgccaaaga cgagttgatc caccgcttcg
     1681 atctgaagcc gcatccggaa ggtggatttt tcagcgagac gtaccggtcc gctgagcctg
     1741 tgcggcgcga cgacggttca atgcagacgc gctcggcgtc caccgcgatt tattacctgc
     1801 tctgtgacgg cgcgcattcg gcgtggcatc ggatcaagtc cgacgaggtc tggcattttt
     1861 atgccggcga gccgttgaac gtccacgtgc tggaccaagc gggcgcgctg atcacacaca
     1921 ggttgggcaa tgcgctgacg cattcggacg ccgtgtttca ggcggtggtg cccgcggggc
     1981 tgtggtttgc cgcggaatgc gcggacccgg cgacgttcgc gctggtgggc tgcacggtgg
     2041 cgccgggttt cgaattcagc gagttcgagc tggcggatgt tgagcctttg aaagcgcagc
     2101 atccgcagca tgcggcgttg attgagcgcc tggggccggg tacctgatcg gcatcgacag
     2161 aaagcggggg ctagggccag ccgcttgatt agcctgggca gacgcagagc ctgcgaccag
     2221 cggcaacacg agcggcaaca aaacacgaag gccggttgcc tgacccgcct cgacgaaagc
     2281 gcaagcacgc cggccgcttg gccagaccga acaacagcgg gaaattgatt caacgatcgt
     2341 ccatcacgac gaccaccctt tatcaccgga acgcattaat ccggtcacgc gtctccatcg
     2401 cgaccttcgc cgcggcctcg gcgaaatcct cgtccttgcc ggcatagata attgcccgcg
     2461 acgagttgat cagcatgccc gtgccgttcg ccgtgcggcc ggcattgacc gtcgcctgaa
     2521 catcgccgcc ttgcgcgccg atgcccggaa tcagcagcgg catatcgccg acgatcccac
     2581 gcaccacttc gatttccttc gggaaggtcg cgccgaccac gagcccgagt tgaccgctcg
     2641 cgttccactt gtccgccgcc agctgcgcga cgacctgata cagcggccgt ccgcccgcct
     2701 ccaggaactg cagatccgag ccgcccgggt tcgaggtccg gcacaacacg atcacgccct
     2761 tgcccgcgtg ctccagatac ggctcgatcg aatcgaaacc catatacgga ttcaccgtga
     2821 ccgcatccgc ctgatagcgc tcgaacgcct cgcgcgcgta ctgctcggca gtgctgccga
     2881 tgtcgccgcg cttcgcgtcc agaatcaccg gcaagccagg atggtgcgca tgaatgtgcg
     2941 cgatcagctg ctcgagctgg tcctccgcgc gatgagcggc gaagtacgcg atctgcggct
     3001 tgaacgacga cgcgtacggc gcggtcgcat cgacgatggt gcggcagaag tcgaaaatcg
     3061 cttcgggacg gttcgcgagc gcgcccggga atttgctggg ctcgggatcg aggccgacgc
     3121 acagcagcga gttcgtgcgc tgccaggcgt cgttgagtgt ctggatgaaa ttggacatgg
     3181 tgagtctcgc ggcgagccga taacggaaga ggcgcgtatt ttacccgtcc tgcgcgggag
     3241 gccgcggcca gacaacatgc caacccgggc tgggcttgcc ccggatcatg acggacattc
     3301 ctccgtttcg cagccgcggt caatgccagc ccagagcgtg gcgccctgcc tcgggccgga
     3361 acgcgcgctc ccgttccgcc gcgccctgct cgcccgctac tgtttccgcc gcgccccgcg
     3421 cgcacccggc atcgaacgca aaccggtgcg ttcgacagtg aagcggaacg tgcgcgtcag
     3481 ccgctcgccc cgtcccagca tcgtcatgcc gttctcgcac gcgccgccgg ccagattcat
     3541 cgcgttgatc ggatgatcga ccggctcgaa gcagaagaag tcctcacccg gtggcgtgta
     3601 cagcacgtag tagtcggtgt cggcggcaat attcagcgac aggcggcgct tgggccacac
     3661 caccgccgcc tgcccgctcc aacccgtgaa cgcatgattg acgatggctt ccggcagggg
     3721 atacgccacg ccgaattgcc atgcgggcgg cgtcggcacg tgacgcaccg gcagccagtc
     3781 gtcgccggag agccacaggc cgccggccgc cgccgacaat tcggtatccg cgtcacgcac
     3841 gaaaaatgga tgcacgccca gcccgaaagg cagcgcctcg cggccggcat tctcgatttc
     3901 cagcgtgatg acgagcgtgg caccgtcgag cgtataggtt tgcgtcgcgc gaaatgcgta
     3961 cggtttgccg tggcggcgat cgagcgtcag acgcacattt tcgcgattcg attgcgcgac
     4021 ctgccaggcg gagagccaac cgtcgccatg aatcggcaag ggctcgtcgg cgcgattttg
     4081 cggtacttcg acacgccgcc ccgccacatt gaaatgaccg ccgccgatcc ggttcgaata
     4141 cggcagcagc ggatagcaag cgagctggtt cggttgggta tccgagccga catggcggca
     4201 gcgccggaaa atcggcgtga gcgcgccgtc gttgcgccag tcgaaacggg tgacgccgcc
     4261 gccgagcatc ggcgcgacgt cgaggcgcaa gtgcgggttg gcgagcgtaa tacaggcgac
     4321 atcgccgccg ctcgtttcgc cgaccgccac cgccgacgtg ctgggcccgg gaagcaccgg
     4381 attggccgcg gcgttgagtc gcgcgcgtcg ggtctgagaa gaagcggaaa caagattcgc
     4441 aacagtcata tatggctcca tcgagaggcg tgggacgttg ccgtcattgc tgacggatgc
     4501 tgttgggcga ttttttttta accggtcgcc tctgggtccg aactgcatgc tgccgatttg
     4561 ctgccactgg ttttgcgaca tcaactgctg attcgccttc aaagtcacgg ctaaaccgcc
     4621 ctccgttacc gcctcgcgac taaggcagcg tcattttgcg ctatctcggc gagccttgga
     4681 acacgggctc cggcaaaccg tgccgtgcgg gcgtggcgat gaatacgccg cccgcaccag
     4741 gatcgcccgc gagcgcggct gcgtcgagat cgatgcgcgc gctcgtaatg tacagcgtgt
     4801 tcagttccgc ctgcccggaa ggaagtccgt ttgggggacc gccaaacgcc acgcaactcg
     4861 gttgcgcggt cggcacctcg acgcgctcgg tttccacacc gtcggggcca taccgcacca
     4921 cgcgcctgcc gccccattgc gcgttccaca ggccgccctc gttatcgacg gtcgagccgt
     4981 cgggctcgcc gctcgcgtcg gtcaaggtcg tgaacacgcg gtcgttgacg atgctgccgt
     5041 ccgcgcaata gtcgcacgtg cggatttcac gggtcggtga gtcgcagtaa tacatcgtcg
     5101 cgccatcggg actgaacgcg atgctgttcg agatcgccgg cgcgggcaac ggcaaccgct
     5161 ccagcgacaa atcatggttc agacgataaa acgcgccgac cgcttgcagc ggcgagcctt
     5221 cgtctttcgt accgaacacg aaacgacctt gacgatcgca gcggccgtca ttcacgcgcg
     5281 tgttcatgcc agcttcgacg tcgacgatat gctgcgtctc gcccgttacc agattgaaga
     5341 acgccagatg cgtcgccagg ccgagcaaca agtgggttgg atcggcacac agcgcgaacg
     5401 tggcgagccg ctcgggcatg ggccatgagg tgctgccgtc gctcgggtca tagcgccata
     5461 ggcgcgcgcc ttcgatatcg gtccagtaga agcgactggt tttcgcgcac cacgtcgcgc
     5521 cttcgccgag cgtgcatttt gtgtcgagca ggagtgccgc gcagggtggc ggcgaccccg
     5581 caggcaagcc cacgcgggtg tccgcactca cgcccagcct ccgtcgacga tgaggtcctg
     5641 cgcggtgatc atcttgctat cgtccgcggc gaggaacagc gccatgcgcg cgaggtcggc
     5701 cggttgcagt tcagcgtcga tacactggcc ttccttgatc gagcggcggc ctgcgtcgtc
     5761 gagccacaaa cgcttctgct tttccgtcat cacccagccc ggcaccagcg tattgacgcg
     5821 gatgttgaag tgaccgaggt cgcgggcgag accacgcgtc aaaccctgca cggccgattt
     5881 cgacatcacg tacaccggat agccgccgtt cttcagcatc cagctaatcg agccgaggtt
     5941 gatgatcgag ccggcgttgg ccgctttcat gtcttcgatc acggcctgcg ccgcgaagaa
     6001 ctgatgacgg atattcaccg cgatgcccgc atcgaaagac tcgcgcgtga cttcagcgat
     6061 cgtgtgacgc ttatcgttcg ctgcgttgtt cacgagcacc tggatcggcc cgagcgcggc
     6121 tttgacatca gcgatcgctt tttgcagcgc gtcgacatca gtcaggtcgc agggcacgaa
     6181 cagcggcttg tgtttcgaat cgccgagttc atcggcgagc gcttcacccg cggtcgcgtc
     6241 gatatcgaag aacgcgacgc gcgcaccctg tgccgcgaaa tgctcgacga acgatgcgcc
     6301 gataccggtc gcgccgcccg taatcaacac cgtgcggtcg acgagactcg gatagcgggc
     6361 aaacgcgttg tccgcgagac ggtcgtttgc attggccgga gacgacatcg ttcgattcct
     6421 cctggagcaa gataagtgga tcgcggcttt aatcgcgcga gccgcggttc ttcaactggt
     6481 cgagcagcac ggcggcgagc aggatcgcgc cgcgcacgag gtactgatag aacgcgtcga
     6541 tgttcatcag gttcatcacg ttctcgaccg tacccatgat cagcacgccg atcacgacac
     6601 cggagatcgt cgcgcgaccg ccgagcagcg acacgccgcc caacacgcac gccgaaatca
     6661 cgttcagttc gaaaccttcc gccgcattcg gctggccgga cgtaatgcgc gaagccagaa
     6721 tcacaccggc tagcgcggtc accgcgcctt ggatcaggaa aataaagacg cgcgtgcgtt
     6781 cgacattgat accggcgaga cgcgaagctt ctggattacc gccgatcgcc agcgtattac
     6841 ggccgtacac ggtttgattg agcatcacgc caaacacgat gaagcacagc agcgtgaccc
     6901 agatcggcag cgatacgccg aagaagctca agccgcccag cgcgatgaac gtatccgacg
     6961 acacacccac ggcctgcccg tgagacacga taaagccgag accgcgaacg atttccatcg
     7021 tggccagcgt cgtgatcagc gcgttgatgc gcagataggc gatcaccgcg ccattgacga
     7081 agccgatcac ggcgcctgcc gccaccgcgg cgatgatcgc gatgaacgta ttgcccgtgg
     7141 cgttcaacac catcgcgcac agcacgcccg cgaacgcgac cgtcgaaccc accgacaagt
     7201 cgaaatcgcg cgaggccaga cagaacatca tcgtgcacgc gaccatgccg atctgcgaga
     7261 tcgacaacgc gagaccgagc atgttctcga tcgagaagaa gtggtcgacc gtcagcgaca
     7321 tcgtgacgaa catcacgatg aagatcagga tcaggctgta ctcggtgatc tgctgccacc
     7381 atttcgcctt gtcgttcgac tgtggaatca acgcttcggc cgcgcctttg gcggcttgtt
     7441 gcgcgaggtt ttctctggct tgcattttga agactcctcc cgttccccac gggttgccgg
     7501 actttcgtcc aaataaggtt caggccgctt gctcggtggc ggcgcttgcg gttccgggca
     7561 gcgcggtcga gctttgcggc aacgcgaggc tcaacaccgt ttgttccgtt gcatcctggc
     7621 gtgccagctc accggaaatc cggccctgac gcatcacgat aatccggtcg gacacgccga
     7681 gcacttccgg caattccgac gaaatcatga cgatcgcgca gccgcgttcg gcaagctgat
     7741 aaatcacgtt atagatctca tgcttcgcgc cgacgtcgat cccgcgcgtc ggctcgtcga
     7801 gaatcacgac cttcagatcg ggctcggcca gccagcgcga cagaatcgcc ttttgctggt
     7861 tgccgcccga cagaaaacga atcttctgcc ggcggctcgg cgtcttgatc ttcagcagct
     7921 tgataaaacg gtcggcggtt tccgcttcct tcttgcggtc gaggaacatc cccgcgcgca
     7981 gatagtgacg gcggcagctg atattgatgt tctccgacac cgtcgccatg gcgacaatgc
     8041 cttcttcctt gcggtcttcc gggcacagca cgatgccgtg cctgatcgct tcgcccgcac
     8101 tgcgcacctt gatcggcttg ccgtcgagca cgatttcgcc gcccttcttg tgatcggcgc
     8161 cgtacacgag gtgcatcagt tcgctgcggc ccgcgcccac cagaccgaaa aagccgacga
     8221 tctcgccgcg ccgcacttcg aagctggccg gctgcacgag cgcatggcct tcgatcgctt
     8281 tcgccgcgaa gcgcacgtcg cccaacggac gtgctgaata gttatagatg tcggagatct
     8341 cgcgcccaac catttcgctc acgatggtgt cgcgcgacac gccttcgagt gtcggatgcg
     8401 aggcaatctt gcggccgtcg cggaagatcg tgcaggcgtc gcaaagctcg tagatctcgt
     8461 ccatgcggtg cgagatgtag atcatcgcgc ggttgtcggc acgcagatcg cgcaccagct
     8521 tgaacagcac ttcagtctcg cgatgcgaca gcgagctggt cggttcatcg agtgcgatca
     8581 cgcgcgcatt acgcagcagc gccttgcaga tttcgaccat ctgccgttgc gcgatcgaga
     8641 gcttacgcag tttggcgttc ggatcgagcc ccacgcccat cgcttcgagg cgttcacgca
     8701 cgaagcgttt cgcttcgcgc ttgttgaccc agccgagcga gttcggcaac tgacccagca
     8761 gcaggttttc cgcgaccgtc agatcgggca cgtattgcag ttcctggtga atcaccgcga
     8821 tgcccgccgc gatcgaggaa gccgcgctcg taaagcgcac ctcattaccg tcgatcatca
     8881 cgcggcccga atcgggctgg tattcaccgc cgagaatttt cagcagggtg gatttcccgg
     8941 cgccgttttc gcccatcagg ccgtgcacct ggccgacgtt gacgtcgaag gacacaccgt
     9001 cgagcgcgcg cacgcctgga aagactttgc cgatattgtc aaaacgtagc gtcgctgaca
     9061 cttcgcctcc tttgtcgacc agagttagcg ctttagcgct tactctggtc ccatgcagtt
     9121 ttgctgcggt cgaccagagt tagcgcttta gcgcttactc ccgtcccatg caacatagtt
     9181 gctgcttcat gtgaaccgtg cttctctaag cgccgcgtgg gcgccaagca cggtctgtct
     9241 caactgctca ttttttattg ctgctgccgg cgcgaccaat ccctggagaa atgacgcacc
     9301 ggcagttcac ttcatgtcac cgcgtgccga atcaaatcgc ctagttcgcc gcgaggccca
     9361 tcttctgacg cacgtcggcc acattgtcgc gcgtggccag catgccggtc gtcagtgtca
     9421 gcatcggcgg ttccttgcct tgcgtgatcc agtcgtacat cagcgtcgag gtttcttcgc
     9481 cgtggcgctt cgggctgatg atcaccgtgc cgtaaaagcc ggtcggcgac ggcttcttga
     9541 actcgttcaa tgccgaatcc gaaccgccga tgccgatacc gatcatgttg tccgccttga
     9601 agccgcggcc ttccgctgcg cgcaccgcgc cgagcacagc ttcgtcgttc agaccgtaag
     9661 cgacccagtg cttgaagttc gggttcttgg tcagcgcgat gttggcggcg ttgaacgcgt
     9721 tttccgtgtc cgtcttcgct tgcggcgcgg cgatgatgtt cgccttcggg aagccggcgg
     9781 cgatcagcgc gtcggtcgcg ccgctggtgc ggtcatgcgc ggtcggcagt tgttcataag
     9841 tcacgtcgat tgcgccgaca tccttcatgt cccagccgcg cttcttgatt tccgcggcca
     9901 ggccgtcgcc cacctgcttg ccgatgttgt acgccgagat gcccatatgc ggcaccgaag
     9961 cgatcggctt gcccgcgccg tcgacgaggc ggtcgtcc
//
LOCUS       DATASH010000307         4295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1823291,
            whole genome shotgun sequence.
ACCESSION   DATASH010000307 DATASH010000000
VERSION     DATASH010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1823291"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VHV99_08640"
     CDS             complement(<1..302)
                     /locus_tag="VHV99_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HEX3635541.1"
                     /translation="MLNRSILSRYDLSAIFARHADVAVGIGILSVLALLIVPVPPFVL
                     DLFICVSFASSLTMLTTTLYVSKAADLASFPSLLLITTLLRLALAIASTKMILLHA"
     gene            complement(304..1044)
                     /locus_tag="VHV99_08645"
     CDS             complement(304..1044)
                     /locus_tag="VHV99_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HEX3635542.1"
                     /translation="MSDKTEAPTPKRIRRAREDGEITKSTHLTMAFSGLVWWLYLFIE
                     APHLYALGTRLILHVTVLDEALPFGDRLVSTLAALTAFLPTVLIALGVGALAVLVPEL
                     MQTRGLIAWKRVTPDVKRLNPVNGLKQMFSLRLLWDTVLTLLQFAVLLFLFVQALATW
                     LRQLAPMWAFSIPVQLGYSAGSHAHLLMAMAASQIAPAAADYFIQRFLLLRSLRMDKS
                     EIKREFRDDEGDPYVKNRRRALHRELGQ"
     gene            complement(1041..1769)
                     /locus_tag="VHV99_08650"
     CDS             complement(1041..1769)
                     /locus_tag="VHV99_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HEX3635543.1"
                     /translation="MSPDYLDYLKGFAFCAIRPAVALYLIPFGQSGSLGILLRLPMVL
                     ALAMLPQHMGWPPHLLPAIGVEAATGVALGLLLGTIFHAAAAAGAVLDQQGGYASAAI
                     YDPHFQQEAALFETLFLQFAALTLFTGPGLPLLFGFFTDAWVLWPPGRWRVDLNDVFR
                     HIALTDLAGTLAEGVRLASPLLGLMLLIDVAFGLMSRHAKRLNPFATARTVKAMILSF
                     AAMLSVPALFAQLRLAFDHVIRLP"
     gene            complement(1766..2041)
                     /gene="sctS"
                     /locus_tag="VHV99_08655"
     CDS             complement(1766..2041)
                     /gene="sctS"
                     /locus_tag="VHV99_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctS"
                     /protein_id="HEX3635544.1"
                     /translation="MNSVPSLASLSSDALMLVFWLSLPALAVATVVGLLVGLLQSVTQ
                     IQDQSLPYGAKIICVGAVLIVTAPWASRELARFLAHVFTFIAAGRLH"
     gene            complement(2038..2682)
                     /locus_tag="VHV99_08660"
     CDS             complement(2038..2682)
                     /locus_tag="VHV99_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HEX3635545.1"
                     /translation="MIAQLDQLQVVGVFFMLGLLPLAVVMTTSFTKFSIVLTLLRSAL
                     GVQQAPSNLVISGLALAATLVVMSPTLAKIENGLPVGDNGEVALPRPAELLQAVRGPL
                     GEFMLAHSRPDERKFLVGAAKRIDPQREAQETDLQLLIPAFLIGEISSGFEAGFLLYI
                     AFLVVDLVVANVLTAMGMVMLSPSTVSIPLKLFIFVSVSGVSKLMHGLIVGYVK"
     gene            complement(2679..3650)
                     /locus_tag="VHV99_08665"
     CDS             complement(2679..3650)
                     /locus_tag="VHV99_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HEX3635546.1"
                     /translation="MTLWPDLETLALADAERHNLAIGHFGRPRAVRVCTGRFTLEFEA
                     CRARYPLRLSGMAAQTPFSATCDAGALMPELAPAVIGARGDTAVTHVADALSDWLCAL
                     EGLFGFTLELTDVAFDAVPPTGAYGIAVTHATSGRAAHFSFCSPAVDAWLRRHPTAPP
                     SGDALLRRLYVRMPICLAGPSMPLPRLRKVAVGDALLFDSQSYYLRVPLRLGACRILL
                     KFTEEHTLIDHVLNDETPPVEVTSELLPIDALTFAFEAVLGTLSLSVAELAHLRQGSI
                     VAFRLPARERSVTLLCQGIPFARGELIDIDGSLGVRVTRLTQADGPA"
     gene            complement(3647..>4295)
                     /locus_tag="VHV99_08670"
     CDS             complement(3647..>4295)
                     /locus_tag="VHV99_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HEX3635547.1"
                     /translation="RLAQQARELKRRAWRHGHAAGKAAVQRQHVGRMAAAAFAAQRLE
                     DRLTQIVLGAVSAIVGDLPPSAALTNQLRRAVAVSQSQRLVSVRVAPAAFEEARQLIA
                     VVEHELAAPFCTVLADAGLPAHSCVIETEAGVIDGGLKVQLRALERDIRDGVAAVLRT
                     YDLADGAGRTTFDRVEQGVRHTLAALAAPRVLPPQQAPVSERVRVVQRPFVREVA"
BASE COUNT          870 a         1423 c         1401 g          601 t
ORIGIN      
        1 tgcgcatgca gcaggatcat cttggtcgac gcgatcgcga gcgcgagacg cagcagcgtg
       61 gtgatcagca gcaacgacgg aaaactcgcg agatccgcgg ccttcgagac atacagcgtg
      121 gtggtcagca tcgtcaggct ggaggcgaaa ctcacgcaga tgaacaggtc gagcacgaac
      181 ggcggcaccg gcacgatcag cagagcgagc acgctcagaa tgccgatgcc gacggcgacg
      241 tcggcgtgac gtgcaaagat cgccgagaga tcgtagcggc tcagaatgga tcgattcaac
      301 atgttattgc ccaagctccc gatgaagcgc gcggcgacgg tttttgacgt aggggtcgcc
      361 ctcgtcgtcg cggaactccc gtttgatttc gctcttgtcc atgcgcaggc tgcgcagcaa
      421 caagaagcgc tgaatgaaat agtcggcggc ggcgggcgcg atctgcgagg ccgccatcgc
      481 catcagcaga tgcgcgtgcg agcctgccga atagccgagc tgaaccggta tcgagaaggc
      541 ccacatgggc gcaagctggc gcaaccacgt ggcgagcgcc tgcacgaaca gaaacagcag
      601 caccgcgaat tgcagcaagg tcagcaccgt gtcccacagc aggcgcaggc tgaacatctg
      661 cttgagaccg ttgaccggat tcagacgctt cacatcgggt gtgacgcgct tccacgcgat
      721 cagtccacgc gtctgcatca actccggcac gagcaccgcg agcgcaccga cgccaagcgc
      781 gatcaacacc gtcggcagga acgcggtcag cgcggcaagc gtcgatacga gccggtcacc
      841 gaacggcagc gcctcgtcca gcaccgtcac gtgcaggatc agccgcgtgc ctagcgcata
      901 caggtgcggc gcttcgataa acagatacag ccaccagacg aggccgctga acgccatcgt
      961 cagatgggtg ctcttcgtga tctcgccgtc ctcacgggcg cggcggatcc gcttgggggt
     1021 tggggcttcg gtcttgtcgc tcatggcaaa cggatcacgt gatcgaaggc cagacgcagt
     1081 tgcgcgaaca gcgcgggcac gctcagcatc gccgcaaacg agaggatcat tgccttgacg
     1141 gtccgtgcgg tggcgaacgg gttgagccgt ttcgcgtgcc gcgacatcag cccgaacgcg
     1201 acgtcgatca gcagcatcag accgagcagc ggactggcga gccgcacgcc ttcggctagc
     1261 gttccggcca ggtcggtgag cgcgatgtgc cggaacacgt cgttcaggtc gacgcgccaa
     1321 cggcccggcg gccacagcac ccacgcatcg gtgaagaagc cgaacaggag cggcagaccc
     1381 ggccccgtga acagggtcaa cgcggcaaac tgcaagaaca gcgtttcgaa gagcgccgct
     1441 tcctgctgga agtgcggatc gtagatcgcc gcgctcgcat aaccgccctg ctgatcgagc
     1501 accgcgcccg ccgctgccgc cgcatgaaag atcgtgccga gcagcaggcc gagcgctaca
     1561 ccggtcgccg cttccacgcc gatggccggc aacaggtgcg gcggccagcc catgtgctgc
     1621 ggcagcatgg ccagcgccag caccatcggc agccgcaaca gaatgccgag cgagccgctc
     1681 tggccgaacg gaatcaggta gagcgccacg gcgggccgga tcgcgcaaaa cgcaaagccc
     1741 ttcaggtagt cgaggtagtc ggggctcaat gcaaccgccc cgccgcgatg aaggtgaaca
     1801 catgggcgag aaagcgcgcg agttcgcggc tcgcccaggg cgccgtcacg atcagcacgg
     1861 cgccgacgca gatgatcttg gccccgtacg gcagactctg atcctggatc tgcgtcaccg
     1921 attgcaacag ccccaccagc aagccgacca cggtcgcgac ggcaagcgcg ggtaacgaca
     1981 accagaacac cagcatgagc gcgtcgctgg acaggctggc aagagacgga acactgttca
     2041 tttgacatag ccgacgatca ggccgtgcat cagcttcgaa acgcctgata ccgacacgaa
     2101 aatgaatagc ttcaacggaa tcgacaccgt gctcggtgac aacatcacca tgcccatggc
     2161 ggtcaacacg ttggcgacga ccagatcgac gaccagaaac gcgatataga gcagaaagcc
     2221 cgcctcgaat ccgctcgata tctcgccgat cagaaacgcc ggaatcagca gttgcagatc
     2281 ggtttcctga gcctcgcgtt gtggatcgat ccgcttcgcg gcgccgacga gaaacttgcg
     2341 ttcgtcgggc cgcgaatgcg ccagcatgaa ttcgccaagc ggaccgcgca ctgcctgcag
     2401 aagttcggcg ggacgcggca acgcgacttc gccgttgtcg ccgacaggca agccgttttc
     2461 gatctttgcc agagttggcg acatcacgac cagcgtcgcc gcgagcgcga gccccgagat
     2521 caccaggttg ctcggcgcct gttgcacgcc gagcgccgag cgcagcagcg tgagtacgat
     2581 gctgaacttc gtaaacgagg tcgtcatcac gaccgcgagc ggtagcaggc cgagcatgaa
     2641 gaacacgccg acaacctgca gctggtcgag ttgggcgatc atgccggacc gtctgcctgg
     2701 gtcagccggg tcacgcgcac gccgagcgaa ccgtcgatat cgatcagctc gccgcgggca
     2761 aacggaatgc cctggcatag cagcgtgacg ctccgctcac gcgccggcag ccggaacgcg
     2821 acgatcgatc cctggcgcag atgagccagt tcggcgaccg acaacgacag cgtgccgagc
     2881 accgcttcga acgcgaatgt cagggcgtcg atcggcagca gttcgctggt cacttcgacg
     2941 ggcggggttt catcattcag gacatggtct atcaaggtgt gctcctcagt gaacttcaac
     3001 aggatccggc acgcgcccaa tcgcaaaggc acgcgcaggt aatacgactg actgtcgaac
     3061 agcagcgcgt cgcccaccgc gaccttgcgc aagcgcggca acggcatcga tgggcccgcg
     3121 aggcagatcg gcatgcggac gtacaggcgg cgcaacagcg cgtcgccgga cggcggcgcc
     3181 gttggatggc gccgcagcca ggcatcgacc gcggggctgc agaaagaaaa atgggcggcg
     3241 cggccactcg tcgcgtgggt gacggcaatg ccataggcgc ccgtcggcgg gacggcgtcg
     3301 aacgccacgt cggtgagttc gagcgtgaag ccgaacaggc cttcgagcgc gcacaaccag
     3361 tcgctcaacg cgtcggcgac atgtgtcacg gcggtgtccc cgcgcgcgcc gatcaccgct
     3421 ggcgccagtt ccggcatgag cgcgccggca tcgcacgtgg cgctgaacgg cgtttgcgcc
     3481 gccatgccgg acaagcgcag tggatagcgt gcccggcacg cttcgaactc cagcgtgaaa
     3541 cggccggtgc agacacgcac cgcccgcggc cggccgaagt ggccgatcgc caggttgtga
     3601 cgctcggcgt ccgccagcgc gagggtttcc agatccggcc agagcgtcat gccacctccc
     3661 gcacgaacgg acgctgaacg acgcgcaccc gctccgacac aggggcttgc tggggcggca
     3721 gcacgcgtgg cgcagccagc gcggccagcg tgtgccgcac gccctgctcg accctgtcga
     3781 acgtggtgcg gcccgcgccg tcggccaggt cgtaagtgcg cagcacggcg gcgacgccgt
     3841 cgcggatgtc gcgttccagg gcgcgcaact gcactttcag accgccatcg atcacgccgg
     3901 cctccgtttc gatgacgcac gaatgcgcgg gtaagccggc atcggccagc accgtgcaga
     3961 acggcgcggc gagttcatgc tcgaccacgg caatcaactg cctcgcttcc tcgaacgccg
     4021 ccggtgccac ccgcacggaa acgagccgtt gcgattgcga gaccgcgacc gcccggcgca
     4081 actgattggt caacgcggcc gacggcggca gatcgccgac gatggcgctc accgccccca
     4141 acacgatctg cgtcaaccgg tcctcgagac gctgcgcggc aaaggccgcc gcggccatgc
     4201 gtcccacgtg ctggcgctgc accgccgcct tgccggccgc gtgcccgtga cgccaggccc
     4261 ggcgtttgag ttcgcgcgcc tgctgggcga gacgg
//
LOCUS       DATASH010000308         7027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3801457,
            whole genome shotgun sequence.
ACCESSION   DATASH010000308 DATASH010000000
VERSION     DATASH010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7027
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3801457"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..317)
                     /gene="iscX"
                     /locus_tag="VHV99_08675"
     CDS             complement(120..317)
                     /gene="iscX"
                     /locus_tag="VHV99_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HEX3635548.1"
                     /translation="MKWTDTQDIAMALTDKHQDVDPQQVRFTDLHRWVTELEGFDDDP
                     NRSNEKILEAIQAAWIEDADY"
     gene            complement(333..674)
                     /gene="fdx"
                     /locus_tag="VHV99_08680"
     CDS             complement(333..674)
                     /gene="fdx"
                     /locus_tag="VHV99_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004193872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HEX3635549.1"
                     /translation="MPQIVVLPHVELCPEGAVIDAVPGKSICDNLLDNGIEIEHACEK
                     SCACTTCHVIVREGFAALTPSEEDEDDLLDKAWGLEPASRLSCQAMVPAEQDLVVEIP
                     RYSINHAKENH"
     gene            complement(813..2684)
                     /gene="hscA"
                     /locus_tag="VHV99_08685"
     CDS             complement(813..2684)
                     /gene="hscA"
                     /locus_tag="VHV99_08685"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HEX3635550.1"
                     /translation="MALLQISEPGMAPAPHQRRLAVGIDLGTTNSLVAAVRSGVPDVL
                     PDEDGHALLPSVVRYLEKGGRRIGRAAKAEAATDPRNTIVSVKRFMGRGKAEVEGAEN
                     APYDFVDAPGMVQIRTVDGVKSPVEVSAEILATLRQRAEDTLGDELVGAVITVPAYFD
                     EAQRQATKDAARLAGLNVLRLLNEPTAAAIAYGLDNGSEGLYAVYDLGGGTFDLSILK
                     LTKGVFEVLAAGGDSALGGDDFDHALYRHVLEQAGIAPQTLAPEDVRLLLDSVRVTKE
                     ALSDAPTATVQARLSSGAEIDLTIEEATFEAITQALVQRTLGPTKKALRDAKVATKEI
                     KGVVLVGGATRMPVIRRAVESLFGQPPLINLDPDQVVALGAAIQADLLAGNRGAEGDD
                     WLLLDVIPLSLGVETMGGLTEKIIPRNSTIPVARAQDFTTFKDGQTAMAIHIVQGERE
                     LVSDCRSLARFELRGIPPMAAGAARIRVTYQVDADGLLSVFAREQGSGVEASVVVKPS
                     YGLADDDIARMLEESFSTAEVDMRARALREAQVEARRLVEATDAALAADAELLDDSER
                     AALDTLLTALRNIAQSDDADAIETATKTLAEGTDEFAARRMNKGIRRALAGRKLDEI"
     gene            complement(2815..3342)
                     /gene="hscB"
                     /locus_tag="VHV99_08690"
     CDS             complement(2815..3342)
                     /gene="hscB"
                     /locus_tag="VHV99_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HEX3635551.1"
                     /translation="MASLNDSHFDLFDLPAQFALDAAALDHAYRTVQAQVHPDRFAAA
                     GDAQKRIAMQWATRTNEAYQTLRDPLKRATYLLHLRGIDVGAENNTAMEPAFLMQQME
                     WREGIEDAAAAKNVDALDVLLTELRDEERMRFDKLGALLDSGANQPAGEAVRQLMFIE
                     RVASEIGTQIERLEN"
     gene            complement(3517..3840)
                     /gene="iscA"
                     /locus_tag="VHV99_08695"
     CDS             complement(3517..3840)
                     /gene="iscA"
                     /locus_tag="VHV99_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018417526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HEX3635552.1"
                     /translation="MAITLTEKAAQHVQKYLTRRGKGVGLRVGVRTTGCSGLAYKLEY
                     VDELAPEDEVFDCNGVKIIVDPKSLAYIDGTELDFAREGLNEGFKFNNPNVKDECGCG
                     ESFRV"
     gene            complement(3990..4406)
                     /gene="iscU"
                     /locus_tag="VHV99_08700"
     CDS             complement(3990..4406)
                     /gene="iscU"
                     /locus_tag="VHV99_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HEX3635553.1"
                     /translation="MAYSDKVLDHYENPRNVGSFAKDDDAVGTGMVGAPACGDVMKLQ
                     IRVGADGIIEDAKFKTYGCGSAIASSSLVTEWVKGKTLDQAMSIKNTQIAEELALPPV
                     KIHCSILAEDAIKAAVADYKQRHGEAAVVEGDKQHA"
     gene            complement(4477..5700)
                     /locus_tag="VHV99_08705"
     CDS             complement(4477..5700)
                     /locus_tag="VHV99_08705"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013698736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HEX3635554.1"
                     /translation="MNNDTLHLPIYMDYSATTPIDPRVVDKMIPYLREQFGNPASRSH
                     SYGWDAERAVEEARENVAALVNADPREIIWTSGATESDNLAIKGAAHFYKSKGKHIVT
                     VKTEHKAVLDTCRELEREGFEVTYLDVKDDGLIDLEKFKAALRPDTILVSVMSVNNEI
                     GVIQDIEAIGEITREKGIIFHVDAAQATGKIAIDLQKLKVDLMSFSAHKTYGPKGIGA
                     LYVRRKPRIRIEAQMHGGGHERGMRSGTLATHQIVGMGEAFRIAREEMATENERIRML
                     RDRLLRGLQDMEETYVNGDMEKRVPHNLNISFNFVEGESLIMAVKDVAVSSGSACTSA
                     SLEPSYVLRALGRNDELAHSSIRFTVGRFTTEQDVDYVINLLKTKISKLRDLSPLWEM
                     HQDGIDISTIQWAAH"
     gene            complement(5775..6305)
                     /gene="iscR"
                     /locus_tag="VHV99_08710"
     CDS             complement(5775..6305)
                     /gene="iscR"
                     /locus_tag="VHV99_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HEX3635555.1"
                     /translation="MRLTTKGRFAVTAMIDLALRQEQGPVTLAGISQRQHISLSYLEQ
                     LFGKLRRHEIVESVRGPGGGYNLARRAEDVTVADIIIAVDEPLDATQCGGKGSCEGTK
                     QHDGHCMTHELWSTLNQKMVEYLDSVSLKDLVDQQRSREGAPAVLRDRRNEAPAVEPA
                     RVVPKGPNSVFNMAGS"
     gene            complement(6436..6933)
                     /locus_tag="VHV99_08715"
     CDS             complement(6436..6933)
                     /locus_tag="VHV99_08715"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HEX3635556.1"
                     /translation="MKTVSVCFVCLGNICRSPTAEGVMRHLVEEARLAERILIDSAGT
                     GDWHIGAPPDERAQQAAGQRGYELAALRGRQVAAADFERFDLLIAMDDKNVAALREVC
                     PADQRDKIRLLMEFVPAADGRWDGAREIVDPYFGGAEGFEQVLDQCEAACRGLVAALR
                     LQLVA"
BASE COUNT         1209 a         2256 c         2124 g         1438 t
ORIGIN      
        1 caggcggaat ttgcgccaga cgcaaaaaag gcgactccat tggagtcgcc tttttctttg
       61 ccgtagccgg cagcggcgtg gagcacatcg ctgaccggct tcgggaaact cagtgacgct
      121 caataatccg cgtcttcaat ccatgcagcc tgaatcgctt cgaggatctt ttcgttcgac
      181 cggttggggt cgtcgtcgaa accctccagc tcggtgaccc agcggtgcaa atcagtgaag
      241 cgcacctgct gcggatcgac gtcctggtgc ttgtcagtca gggccatcgc gatgtcttgc
      301 gtatcggtcc acttcatggc tgcatcctcc agttagtgat tttccttcgc gtgattgatc
      361 gagtagcgcg ggatctcgac gaccagatcc tgctcggccg gcaccatcgc ctggcacgac
      421 agacgcgagg ccggttcgag cccccacgcc ttgtccagca gatcgtcctc gtcttcctcg
      481 gacggcgtca aggcggcgaa accctcacgc acgatcacgt gacaggtggt gcatgcgcag
      541 gacttctcgc aggcgtgctc gatttcgatg ccattgtcga gcagattgtc gcagatgctc
      601 ttgccgggca cggcatcgat caccgcgcct tccgggcaca gttcgacgtg aggcagcaca
      661 acgatttgag gcatacattt tccgtttggg tctgcggcac ggctgctgca aacaggctcc
      721 gtaccatttt actggcgccg cgcggacttt ttaagtccgc gcggcgcggt tcattgccgg
      781 ctggcgcgca gggcgcggcc acgccgcaag gttcagatct cgtcgagctt gcggcccgcc
      841 agcgcgcggc gaatgccctt gttcatgcgg cgggcggcga attcgtcggt gccttcggcg
      901 agggttttgg tcgcggtttc gatcgcgtcg gcgtcgtcgc tttgcgcgat gttgcgcaac
      961 gcggtaagca gcgtgtcgag tgcggcgcgt tcgctgtcgt cgagcaattc ggcgtcggcg
     1021 gcgagcgcgg cgtcggtggc ttccacgaga cggcgcgctt ccacttgcgc ttcgcgcaaa
     1081 gcgcgagcgc gcatgtcgac ttcggcggtc gaaaagctct cttccagcat cctggcgatg
     1141 tcgtcgtcgg cgagaccgta ggacggcttc accaccaccg aggcttccac gcccgaaccc
     1201 tgttcgcgcg cgaacacgga cagcaagccg tccgcgtcga cctgatacgt gacgcgaatc
     1261 cgcgccgcgc cggcggccat cggcggaatg ccgcgcaact cgaaacgcgc gagcgaacgg
     1321 caatcgctga cgagttcgcg ttcgccttgc acgatgtgga tcgccatcgc ggtctggccg
     1381 tccttgaagg tcgtgaaatc ctgcgcacgg gcgaccggaa tcgtcgaatt gcgcgggatg
     1441 atcttctcgg tcaaaccacc catggtttcg acgccgagcg acagcggaat cacgtcgagc
     1501 agcagccaat cgtcgccctc cgcgccgcga ttgcccgcga gcagatcggc ctggatcgcc
     1561 gcgcccaacg cgacgacctg atccgggtcc agattgatca gcggcggctg gccgaacaac
     1621 gattcgaccg cgcggcggat caccggcatg cgcgtcgcgc cgccgaccag caccacgcct
     1681 ttgatttctt tggtagcgac tttggcgtcg cgcagtgcct tcttcgtcgg cccaagcgtg
     1741 cgctgaacca gcgcctgagt gatggcttcg aaagtggcct cctcgatcgt cagatcgatc
     1801 tcagcgccgc tcgagagcct ggcctgcacc gttgcagtgg gtgcgtcgga taacgcctcc
     1861 ttggtcacgc gtacgctgtc gagcagcaga cggacgtctt ccggtgcgag cgtttgcggc
     1921 gcgatgcccg cctgctccag cacgtggcga tacagcgcgt ggtcgaaatc gtcgccgccg
     1981 agcgcggagt cgccgcccgc cgcgagcact tcgaacacac ctttggtgag cttcagaatc
     2041 gacagatcga aggtgccgcc gccgaggtcg tagaccgcgt agaggccttc ggaaccgtta
     2101 tcgaggccgt aggctatcgc agccgcagtg ggctcgttca gtaggcgcag cacgttcaga
     2161 ccggccagac gcgcggcgtc tttggtcgcc tggcgctgcg cttcgtcgaa atacgcgggt
     2221 accgtaatga ccgcgccaac cagttcgtcg ccgagcgtat cttcggcacg ctggcgcagc
     2281 gtcgcgagaa tttcggccga cacttccacc ggactcttca cgccgtcgac ggtacggatc
     2341 tgcaccatgc cgggtgcgtc gacgaaatcg tagggcgcgt tctcggcgcc ttccacttcc
     2401 gccttgccgc ggcccatgaa acgcttgacc gagacgatcg tgttgcgcgg atcggtcgcc
     2461 gcttcagcct tggccgcgcg gccgatacgg cggccgccct tttccaggta acgcaccacc
     2521 gacggcaaca gcgcatggcc gtcttcgtcg ggcagcacgt cgggcacgcc gctgcgcacg
     2581 gcggcgacga gggaattggt ggtgccgaga tcgataccga ccgccagacg ccgctgatgg
     2641 ggcgccggcg ccatgccggg ttcggagatt tgcagtaggg ccatctggat cttctcgggg
     2701 ccgctggacc ccgtcctgaa ttttcgcgtt gctggatgtc gtgctacgac tgctatggct
     2761 gcttattcat gcgcggcact gtggcactgt ggcgcacgcc ggaccgccgg accgctagtt
     2821 ctcgagccgc tcgatctgcg tgccgatttc cgacgccacc cgctcgatga acatcaactg
     2881 ccgcaccgcc tcgcccgccg gctgattcgc gccgctatcg agcaatgcgc cgagcttgtc
     2941 gaaacgcatg cgctcttcgt cgcgcagttc ggtcagcaag acgtcgagcg cgtcgacgtt
     3001 ctttgccgcg gccgcatctt caataccttc acgccactcc atctgctgca tcaggaacgc
     3061 cggctccatc gccgtgttgt tctccgcgcc gacgtcgatg ccgcgcagat gcagcagata
     3121 ggtcgcgcgc ttcaacggat cgcgcagcgt ctggtacgcc tcgttggtgc gcgtggccca
     3181 ttgcatcgcg atgcgtttct gcgcgtcgcc ggctgccgcg aagcggtccg gatgcacttg
     3241 cgcctgcacc gtgcggtagg cgtgatcgag cgccgctgcg tcgagcgcga attgcgccgg
     3301 cagatcgaac agatcgaagt ggctgtcgtt cagcgaggcc atcaaataga aggagtccgt
     3361 tcaggaaagc acaccggagt gcgggctaaa acgcggatcg aaagcgccgc gaaattcagt
     3421 ggcgcgctca gttgcaagac accggctcga ttgtgcggat gaaactaaaa aggcggcccg
     3481 cgccgccttt gatccgctaa cgcggaatga gccgatttac acgcggaacg attcgccgca
     3541 accgcactcg tccttcacgt tcgggttgtt gaacttgaag ccttcattca acccctcgcg
     3601 ggcgaagtcg agttcggtgc cgtcgatata cgcgaggctc ttcgggtcga caatgatctt
     3661 cacgccgttg caatcgaaca cttcgtcttc gggtgcgagt tcatccacgt actcgagctt
     3721 gtaggccaag cccgagcacc ccgtcgtgcg cacgcccacg cgcaagccga cgcccttgcc
     3781 gcgacgagtc agatacttct ggacgtgctg tgctgccttt tcggtcaacg taattgccat
     3841 agcgtttctc attgcgccgc gcgtgttcgg cattcatctg cctggtgacg cgctgctgcc
     3901 ctgcctgcat caaatatcgc tcatgccgga cggtgcattt ctgctcgcac cgcccgtttt
     3961 tggtggctca aaagagcacc caagcgcgct tacgcgtgtt gcttgtcgcc ttcgacgaca
     4021 gccgcttcac cgtggcgctg cttgtagtcg gcgaccgctg ccttgatcgc gtcttccgcg
     4081 aggatcgagc agtggatctt caccggcggc agcgccagtt cttcggcgat ctgcgtgttc
     4141 ttgatcgaca tggcctgatc gagcgtcttg cctttcaccc attcggtgac gagcgagctc
     4201 gaggcgattg ccgaaccgca gccgtacgtc ttgaacttcg cgtcttcgat aatgccgtcc
     4261 gcgcccacgc ggatctgcag cttcatcacg tcgccgcatg ccggtgcgcc gaccatgccg
     4321 gtgccgactg catcgtcgtc cttcgcgaag gaaccgacgt tgcgcgggtt ttcgtagtgg
     4381 tccagaacct tgtcgctata agccatgatg acactccttg atccgtttca acctgcaaat
     4441 tgcaattcga tgaattcgac gtccacaact ggcgcgtcag tgcgctgccc actggatggt
     4501 cgagatatcg atcccgtcct ggtgcatttc ccacagcggc gacaaatcgc gcagctttga
     4561 aatcttggtc ttcagcaggt tgatcacgta atcgacatcc tgctcggtcg tgaagcggcc
     4621 caccgtgaag cggatcgagc tgtgcgccag ttcgtcgtta cgtcccagtg cgcgcagcac
     4681 atacgacggt tccagcgaag ccgacgtaca ggccgagccc gacgacaccg ccacgtcctt
     4741 caccgccatg atcagcgatt cgccttcgac gaaattgaag ctgatgttca ggttgtgcgg
     4801 cacacgcttt tccatgtcgc cattcacata cgtttcttcc atgtcctgca ggccgcgcag
     4861 cagacggtcg cgcagcatgc ggatacgctc gttttccgtc gccatttctt cacgggcgat
     4921 acggaacgct tcacccatac cgacgatctg gtgcgtggcc agcgtgcccg aacgcatgcc
     4981 gcgctcgtga ccgccgccgt gcatctgcgc ttcgatccgg atacgcggct tgcggcgtac
     5041 atacagcgcg ccgatgccct tcggaccata cgtcttgtgc gccgagaacg acatcaggtc
     5101 gaccttcagc ttttgcaggt cgatggcgat cttgccggtg gcttgcgccg cgtcgacgtg
     5161 gaaaatgatg cccttttcgc gggtgatctc gccgatcgcc tcgatgtcct ggatcacgcc
     5221 gatctcgttg ttcaccgaca tcaccgaaac cagaatcgta tccgggcgca gtgcggcctt
     5281 gaacttctcg aggtcgatca ggccgtcgtc cttcacatcc aggtacgtga cttcaaagcc
     5341 ttcgcgctcc agttcgcggc aggtgtcgag cacagccttg tgctcggtct tcaccgtgac
     5401 gatgtgcttg cccttgctct tgtagaagtg cgcggcaccc ttgatcgcga ggttgtccga
     5461 ctccgtggcg cccgaggtcc agataatttc gcgcggatcg gcattcacca gcgcagcgac
     5521 gttctcgcgc gcttcctcaa ccgcacgctc cgcatcccag ccatatgagt ggctgcgcga
     5581 tgcggggttg ccgaactgct cacgcaggta cggaatcatc ttgtccacca cacgcggatc
     5641 gatcggcgtc gtcgcgctgt agtccatgta aatgggcagg tggagagtgt cgttattcat
     5701 caattgctcc ggggacgtca ttgctctggc ttctgggttc ctgatcaggc tgcggtgcgt
     5761 tcagattgcc gcgcctagga accggccatg ttgaaaacag aattaggccc tttcggcacg
     5821 acgcgggcgg gttcgaccgc cggcgcctcg ttgcgccggt cgcgcaacac cgcgggcgcg
     5881 ccttcgcgcg aacgctgctg gtcgaccaga tctttcaggg agaccgaatc caggtactcg
     5941 accattttct ggttcaacgt cgaccacaat tcgtgcgtca tgcagtggcc gtcgtgctgt
     6001 ttggtgccct cgcacgagcc cttgccgccg cactgggtag cgtcgagtgg ttcgtcgacc
     6061 gcaatgatga tgtcggcgac ggtcacgtct tcagcacggc gggccagatt gtagccgccg
     6121 cccggtccgc gcacggactc gacgatttcg tgacgacgca gcttgccaaa cagctgctcg
     6181 agataagaca gggagatatg ttggcgctgg ctgatacccg caagcgtcac cgggccctgc
     6241 tcctggcgca gtgccaggtc aatcatcgcc gtgacggcga aacggccttt cgtggtgagt
     6301 ctcatatggt gtgggaaccg caattctcga caagtttggt caagtataaa tacatgactg
     6361 aattagtcaa gtatccctac cgcgatttgg gtcatttact taattgttgg ataaggcagc
     6421 ggcaggccac tcgccctagg caaccaactg gagacgcagc gcggcgacaa gcccgcgaca
     6481 ggctgcttcg cattgatcca gtacctgctc gaagccttcc gcgccgccga aataagggtc
     6541 gacaatttcg cgggctccgt cccagcgacc atctgccgcg ggcacaaatt ccatcagcag
     6601 acggattttg tcgcgctgat ccgccggaca aacctcacgc agtgcggcga cgtttttgtc
     6661 gtccatcgca atcagcaggt cgaagcgctc gaagtcagcc gcggcgacct ggcgaccgcg
     6721 caacgcagcc agttcataac cccgctgccc ggccgcctgc tgcgcgcgct cgtcgggcgg
     6781 cgcgccgatg tgccagtcgc ccgtgccggc ggaatcgatc agaatgcgct ccgcgagcct
     6841 cgcctcttcg accagatggc gcatcacgcc ttccgccgtc ggcgaacggc agatgttccc
     6901 gaggcatacg aagcagacgg atacggtttt catcagaagt ttcgggcgat ccagcgcgca
     6961 acgcgtggag actgacggtt agcaaacggt gccgcgatta tacaaagctg ggcggattct
     7021 cgccggc
//
LOCUS       DATASH010000309         6379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6965775,
            whole genome shotgun sequence.
ACCESSION   DATASH010000309 DATASH010000000
VERSION     DATASH010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6379
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6965775"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..113)
                     /locus_tag="VHV99_08720"
     CDS             complement(<1..113)
                     /locus_tag="VHV99_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004193086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX3635557.1"
                     /translation="MSRRRVVVTGLGLISPVGNNVADGWANLVAGKSGIAN"
     gene            complement(413..652)
                     /gene="acpP"
                     /locus_tag="VHV99_08725"
     CDS             complement(413..652)
                     /gene="acpP"
                     /locus_tag="VHV99_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012735022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX3635558.1"
                     /translation="MDNIEQRVKKIVAEQLGVAEAEIKSEASFVNDLGADSLDTVELV
                     MALEDEFGMEIPDEEAEKITTVQQAIDYARANVKA"
     gene            complement(804..1553)
                     /gene="fabG"
                     /locus_tag="VHV99_08730"
     CDS             complement(804..1553)
                     /gene="fabG"
                     /locus_tag="VHV99_08730"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEX3635559.1"
                     /translation="MEKTLDKQIAIVTGASRGIGRAIAMELARQGATVIGTATSESGA
                     AAITEAFGAAGVSGRGAVLNVNDAAAAEALIDGTVKEFGALHVLVNNAGITQDQLAMR
                     MKDDDWDAVIDTNLKSVFRLSRAVLRPMMKAKGGRIINITSVVGSAGNPGQVNYAAAK
                     AGVAGMTRALAREIGSRGITVNCVAPGFIDTDMTKTLPEEQQTALKTQIPLGRLGSPD
                     DIAHAVAFLASPQAGYITGTTLHVNGGMYMS"
     gene            complement(1640..2575)
                     /gene="fabD"
                     /locus_tag="VHV99_08735"
     CDS             complement(1640..2575)
                     /gene="fabD"
                     /locus_tag="VHV99_08735"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX3635560.1"
                     /translation="MKFAFVFPGQGSQSVGMLNAFADHAVVRETVQEASDALDQDLGK
                     LIAEGPVEDLNLTTNTQPVMLTAAYAIYRAWQQTGGPKPAIVAGHSLGEYTALVAAGA
                     LAFRDAVPLVRFRAQAMQTAVPVGVGGMAAILGLDDDTVRAVCAEASVAGVVEAVNFN
                     APAQVVIAGHKAAVEKACEVAKAKGAKRALPLAVSAPFHSSLLKPASDQLREYLAGID
                     VQVPSIPVLNNVDVAVVNEPAWIKDALVRQAAGPVRWVESVQAMAAQGVTHVVECGPG
                     KILAGLTKRIDGNLVGASVFDPASLEETLKLVTAA"
     gene            complement(2687..3676)
                     /locus_tag="VHV99_08740"
     CDS             complement(2687..3676)
                     /locus_tag="VHV99_08740"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010105339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEX3635561.1"
                     /translation="MAQSTIYSRVIGTGSYLPPGRITNQDLAERLASQGVETSDEWIV
                     ARTGIHARHFAEPDVTTSDLALIASQRAIEAADIDPQSIDLIIVATSTPDFVFPSTAC
                     LLQNKLGIKNHGAAFDVQAVCSGFAYAVATADSFIRSGQHRTALVIGAETFSRILDFK
                     DRTTCVLFGDGAGAVILQASEEPGVLASALHADGSHSNILCTPGNVNGGVIEGSAFLH
                     MDGQAVFKLAVNVLEKVAVEALAKANLSAGQVDWLIPHQANIRIMQSTCRKLGLPQER
                     MVVTVGEHGNTSAASIPLAFDVAVRDGRIKRGQNVLIEGVGGGFTWGASVIRY"
     gene            complement(3676..4782)
                     /gene="plsX"
                     /locus_tag="VHV99_08745"
     CDS             complement(3676..4782)
                     /gene="plsX"
                     /locus_tag="VHV99_08745"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006764798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEX3635562.1"
                     /translation="MTVKLTIDCMGGDHGPSVTVPAAVNFVRSHPDAQLLLVGIESAI
                     RAQLKKLKAQDLPALTVVPASEIVAMDDPVEVALRKKKDSSMRVALNRVKEGEAQACI
                     SAGNTGALMAVSRYVLKTLPGIERPAIAFALPNPDGYTTMLDLGANVDCEPQHLLQFA
                     EMGHALVSALEGKERPTIGLLNIGEEVIKGNDTIKRAGELLRASTLNFHGNVEGNDIF
                     KGTVDVIVCDGFVGNVALKTSEGLAQMLNDIIKEEFGRSWLTKVMAVLALPVLMRFKK
                     RVDHRQYNGAALLGLRGLVIKSHGSADAYGFEWAIKRGYDAVKNGVLERLARAMEENA
                     GSLEQAARDASGAGHASPIAGQPAEPYAAQSSKA"
     gene            complement(4955..5134)
                     /gene="rpmF"
                     /locus_tag="VHV99_08750"
     CDS             complement(4955..5134)
                     /gene="rpmF"
                     /locus_tag="VHV99_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEX3635563.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDFLTAAPLAVEPSTGEVHLRHHVSP
                     NGYYRGKKVVKTKND"
     gene            complement(5236..5880)
                     /locus_tag="VHV99_08755"
     CDS             complement(5236..5880)
                     /locus_tag="VHV99_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEX3635564.1"
                     /translation="MTQHPGNPAGLSDPHELDLFEFSRSGRQAAGVLRVSQLPRMLNE
                     VPAEAPDRDAAFTWQAEGATQPELQDDGSEGPQPYLRMAIHGAAWLECQRCMTPYSQA
                     FNVDAIYRIVNTEAEAEEFPLDEDEVEVIVGSRQFDLIDLIEEELLLSLPLVPKHEVC
                     PEVHESLVSGVAGAEGEGDEAAPDEAGEGGKPERPNPFAALESLKRGEPGDKKH"
     gene            6140..>6379
                     /locus_tag="VHV99_08760"
     CDS             6140..>6379
                     /locus_tag="VHV99_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEX3635565.1"
                     /translation="MPDSPKRPPRLILASSSPYRRELLERLRVPFDVVVPAIDETPLA
                     GETPEATALRLADAKARAAASSLAAADAALVIGSDQ"
BASE COUNT         1215 a         2050 c         1854 g         1260 t
ORIGIN      
        1 atattggcaa tacccgactt gccggcgacc agattggccc agccgtcggc aacattattg
       61 ccaacaggcg aaatcagccc caggcctgta acaacaacac gacggcggct cacggtaacc
      121 cctttttcat agatgacaaa aacaaaagcc acagcggcca caggaacctg acctgtctgc
      181 cctgtggctg ttaagccgcc cacacacctg gtgtttcaca ccaggtcccc cgaaggggca
      241 agaaaacctg gggcggcccg gcgttttctt gaaagtcggc aatcgcgcgg aaagttgcgc
      301 tgtcaacatc gctggctcct gcacggcaaa aagcgccagc cctgctggca tcggaaaccg
      361 acacggcagg gcgtcatacg ccgccaacgc agaaacgcag gcgcgaatga ctttaggcct
      421 tgacgttcgc gcgagcgtag tcgatcgctt gctggacggt agtgatcttc tcggcttctt
      481 catccggaat ttccatgccg aattcgtctt cgagggccat cacgagttca acggtgtcga
      541 gcgagtcggc gccgaggtcg ttcacgaacg aagcttcgct cttgatttca gcttctgcaa
      601 cgcccagttg ttctgcgacg atcttcttga cgcgctgttc gatattgtcc attacccctc
      661 caagggaaaa gaagttcaaa aatacaggtg cgcgcatttt atcaggtttg acccggcaaa
      721 aaagcggcgc atcgggttcc tgtcaaggca tgcgccttat gcttttgcaa acgcatcaag
      781 gcgcggatag taaccgaatt tggttacgac atgtacattc cgccgttcac atgcagtgtc
      841 gtgccggtga tataacccgc ttgcggcgac gccaggaacg ccacggcgtg cgcgatatcg
      901 tccgggctgc caaggcggcc gagcggaatc tgcgtcttca gtgcggtttg ctgctcttcc
      961 ggcagggtct tggtcatgtc ggtatcgatg aagcccggcg cgacgcaatt cacggtgatg
     1021 ccacgactgc caatctcgcg tgcaagtgcg cgcgtcatgc ctgctacgcc cgctttcgcg
     1081 gctgcgtaat tgacctgccc cgggttgccg gccgagccga ccaccgacgt gatgttgatg
     1141 atccggccgc ccttcgcctt catcatggga cgcagcaccg cgcgcgacag gcggaagacc
     1201 gacttgagat tggtgtcgat caccgcgtcc cagtcgtcgt ccttcatgcg cattgccagc
     1261 tggtcttgcg taatgccggc gttgttcacc agcacatgca gcgcgccgaa ctccttcacg
     1321 gtgccgtcga tcagcgcttc cgctgcggcg gcgtcattta cgttgaggac cgcaccgcgg
     1381 ccgctcacgc cggccgcacc aaacgcttca gtaatcgcgg ctgcgccgct ttcgctggtc
     1441 gccgtgccga tcaccgtcgc gccctggcgc gccagttcca tcgcgattgc gcgaccaatg
     1501 ccgcgcgagg cgccggtcac gattgcgatc tgcttgtcga gagtcttttc catctgtcag
     1561 tccggatgcc ctttcggagg gctgattgat tcttgaagtc gatttttggc gccgccagtt
     1621 gctgcgtatg acgcggcgct taggccgccg tcacgagctt gagcgtttct tcgagcgatg
     1681 ccggatcgaa caccgatgca ccaaccagat tgccgtcgat acgcttggtc aaacccgcaa
     1741 ggatcttgcc cggtccgcat tcgacgacgt gcgtgacgcc ttgcgccgcc atcgcctgca
     1801 cgctttccac ccaacgcact ggacctgcgg cctggcgtac cagcgcgtcc ttgatccagg
     1861 ccggctcgtt gaccaccgcg acgtcgacat tgttgagcac tggaatcgac ggcacctgca
     1921 cgtcgatgcc agccagatac tcacgcaatt gatccgacgc cggtttaagc agcgacgagt
     1981 ggaacggcgc cgataccgcc agcggcaatg cacgctttgc gcccttcgcc ttcgcgactt
     2041 cgcaagcctt ttcgactgcg gccttgtgac cggcgatcac gacttgcgcc ggcgcgttga
     2101 aattcaccgc ctcgacaaca cccgcgaccg acgcttcggc gcataccgcg cgcaccgtgt
     2161 cgtcatcaag gccgagaatc gcggccatgc cgcccacgcc gactggaacg gccgtttgca
     2221 tggcttgcgc acgaaaacgc acgagcggca cagcatcgcg aaacgcgagc gcgcccgccg
     2281 cgaccagcgc ggtgtactca ccgagactat gaccggcaac gatggccggc ttcgggccgc
     2341 ccgtctgctg ccacgcgcga taaatcgcgt acgcggcggt cagcatgacc ggctgggtat
     2401 tggtagtgag attcagatct tcgacggggc cttcggcgat cagcttgccg aggtcctgat
     2461 cgagcgcatc ggatgcttcc tgaaccgtct cacgcacgac cgcgtgatcg gcgaatgcat
     2521 tgagcatgcc gaccgactgc gaaccttgcc caggaaaaac gaacgcaaat ttcatatcgt
     2581 ccccaaaatc gatgttgtca gatgtgggtg gcgcgcccga tggattgaga gcgcgcttaa
     2641 gcgtagccag gcgccacgaa gagcgctcgc cataacgtcg ccgctatcag tagcggataa
     2701 ccgacgcgcc ccacgtgaag ccgccgccga cgccttcgat cagcacgttc tggccgcgct
     2761 tgatgcggcc gtcgcgcact gcgacgtcaa atgcgagcgg aatggacgca gccgacgtat
     2821 tgccgtgctc gcccaccgtg acgaccatgc gctcctgcgg caagccaagc ttgcgacagg
     2881 tactttgcat aatgcggatg ttggcctggt gcgggatcag ccaatcgacc tgcccggccg
     2941 acagattggc cttggcgagt gcctcgaccg cgaccttctc gagcacattg acggcgagct
     3001 tgaacaccgc ctgcccgtcc atgtgcagga acgcgctgcc ttcgatcacg ccgccgttca
     3061 cattgccagg cgtgcagaga atgttcgaat ggctgccgtc ggcgtgcaat gcgctagcca
     3121 gcacccctgg ttcttcggat gcctgcagga tcaccgcgcc cgccccatcg ccgaacagca
     3181 cgcacgtggt gcggtccttg aaatcgagaa tgcgcgaaaa ggtttccgcg ccgatcacga
     3241 gcgccgtgcg atgctggcca ctgcgaatga aactgtccgc tgtcgccacc gcataagcga
     3301 agccggaaca gacggcctgt acgtcgaatg ccgcgccgtg attcttgatg ccgagcttgt
     3361 tctgcaacag gcacgccgta ctcggaaaca caaagtccgg cgtggaggtt gcaacgatga
     3421 tcagatcgat ggactgcgga tcgatatcag ccgcttcgat cgcgcgctgc gaggcgatca
     3481 gcgccagatc gctggtggtg acatcgggtt cggcgaagtg gcgcgcatgg atgcccgtgc
     3541 gggcaacgat ccactcgtcg ctcgtctcga cgccttggct ggcaagacgt tcagccagat
     3601 cctgattggt gatgcggccg ggcggcagat agctgccggt gccgatcacg cgggaataaa
     3661 ttgtggattg agccattatg ccttcgagga ttgcgcagcg tagggctcgg ccggctggcc
     3721 tgcgatcggg cttgcgtgac ccgcaccgct tgcgtcgcgc gccgcctgct caagagaacc
     3781 tgcgttctct tccattgctc gcgcaaggcg ttccagcacg ccgtttttga cggcatcata
     3841 cccgcgtttg atagcccact caaacccgta ggcatccgcc gagccatggc ttttgatcac
     3901 cagcccgcgc aggcccagca atgcggcccc attgtattgc cgatggtcga cgcgcttctt
     3961 gaagcgcatc aataccggta acgcgagcac cgccatcacc ttggtgagcc acgagcggcc
     4021 gaactcttcc ttgatgatgt cgttgagcat ctgcgcgagg ccttctgacg tcttcagcgc
     4081 gacattgccg acaaaaccat cgcagacgat gacgtcgacc gtgcccttga agatgtcgtt
     4141 gccttcaacg ttgccgtgaa agttaagtgt actcgcgcgc agcaattcgc cggcacgttt
     4201 gatggtgtcg ttacccttga tgacttcttc accgatgttg agcaggccga tggtgggccg
     4261 ctccttgcct tcgagcgccg acacgagtgc gtgccccatc tccgcgaact gcagcagatg
     4321 ctgcggctcg cagtcgacgt tggcgcccag gtcgagcatc gttgtgtagc catcgggatt
     4381 gggcagcgcg aacgcgatgg ccgggcgctc aatgcccggc agcgttttca gcacatagcg
     4441 cgataccgcc atcagcgcac cggtgttgcc ggcggaaatg caggcttgcg cctcgccttc
     4501 cttgacgcgg ttcagcgcca cgcgcatgga cgaatctttt ttcttgcgca gcgcgacttc
     4561 aaccggatcg tccatggcga cgatctcgga agcgggcacg acggtcagcg ccggcaggtc
     4621 ttgagccttc aacttcttca gttgcgcacg aatcgcactt tcaatgccga cgagcagcag
     4681 ttgcgcgtcg ggatgcgaac gaacgaagtt aacggcagcg ggaacggtca cggacgggcc
     4741 gtggtcgcct cccatgcaat ctatcgtgag ctttactgtc atggagtgcg acgaatttca
     4801 gacgccctgc atgggaccgc aataggcgct aaaccgccaa ctgcgatcga cacaaaaaag
     4861 cggcagttga atgccgcctt tttgccgagc cgggaaaatg tcaagcgagc cgatcgccac
     4921 gcgaaacgcc aaggggcgca acgcagtgaa acgattagtc gttcttcgtc ttgacgactt
     4981 tcttgccgcg atagtagccg ttcgggctaa cgtggtgacg cagatgcact tcgcccgtgc
     5041 tcggctccac ggccagcggc gctgccgtca ggaaatcgtg cgaacggtgc atgccgcgct
     5101 tcgacggcga cttcttattt tgttgaactg ccatgactaa ctcctaaaaa ttttccgaat
     5161 tctaacacag cccgacccgg tgtccgccat tgaccgaatg gccaatgcgc ccgcttcgcg
     5221 ctcccatgca actgttcagt gcttcttgtc gcccggctca ccgcgcttga gactttcgag
     5281 cgccgcaaac ggattgggcc gctcgggctt tccgccctca ccagcttcgt ccggcgcagc
     5341 ctcgtcgccc tcgccttctg ctccagccac accagagacg aggctctcgt gcacttcggg
     5401 acacacctca tgcttgggca cgagcggcaa ggaaagcagc aactcttctt cgatcaagtc
     5461 gatgagatcg aactggcgcg agcccacgat cacttcgact tcatcctcgt cgagcggaaa
     5521 ctcttcggct tccgcctcag tgttgacgat acggtaaatt gcgtcgacgt tgaacgcctg
     5581 cgaatacggt gtcatgcacc gctgacattc gagccatgct gcgccgtgaa tcgccatcct
     5641 caaataaggc tggggaccct cgctgccgtc gtcctgcaat tccggctgcg tcgccccttc
     5701 ggcttgccag gtgaacgcgg catcgcggtc tggcgcttct gccgggactt cgtttaacat
     5761 gcgcggcagt tgcgagacgc gcaggacacc cgcggcttgc cgcccactcc gcgaaaattc
     5821 gaacagatcg agttcatgcg ggtcggacag acctgcaggg ttgccaggat gttgagtcat
     5881 gtgcgctcct gcgtcggcga ggatttcgcc ggagataaac cgcgaggtta aaccggtttg
     5941 cgtgacagcc tgcgaagccc acggcgcggt gtccagattg agttcaatcg aacacaccga
     6001 gacccgccta gcgcaagcat accgatgaaa agcccaaaat catatccgtt ttgtcttttc
     6061 gagtcaaaca cttaagcccg tacgcgcacg agctccgcgc gagcgccgta cgccccgcct
     6121 ccctagccgt tggtcgatca tgccagattc ccccaaacgc ccgccacgcc tcattctggc
     6181 gtcgagttcg ccgtatcggc gcgagttgct cgaacgcctg cgcgtgccgt tcgacgtcgt
     6241 cgtgccagcg attgatgaga cgccgctcgc cggcgaaaca cccgaagcga ccgccttgcg
     6301 acttgccgac gccaaagcgc gtgcggccgc cagctcactc gccgcggccg acgccgcgct
     6361 ggtgatcggc tccgaccag
//
LOCUS       DATASH010000310         2760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_438240,
            whole genome shotgun sequence.
ACCESSION   DATASH010000310 DATASH010000000
VERSION     DATASH010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438240"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(249..971)
                     /locus_tag="VHV99_08765"
     CDS             complement(249..971)
                     /locus_tag="VHV99_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HEX3635566.1"
                     /translation="MSAPEAIEFQPAADIDAKASEPPRLDQGELKARQQRSRRATFVK
                     WLRKVHGWIGLWGAALGLLFGTTGFLLNHRGGPLKVSTGEPRVSVVQVPLPQPAPQTP
                     RDLARWLKHELKLAGNPGRAQKEPARPVAWGDRTMVQPEHWQLNFAAPHENTAAEYWV
                     GNGYVTVKRSENSFLAMLTNLHKGVGLSVGWVLLIDTLAGSIILLSLTGVLLWTELNK
                     RKTIGAMLVTGSIVAALCMGLL"
     gene            complement(968..1651)
                     /locus_tag="VHV99_08770"
     CDS             complement(968..1651)
                     /locus_tag="VHV99_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA];
                     GO_function: GO:0031418 - L-ascorbic acid binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HEX3635567.1"
                     /translation="MLLHIPNVLNAEQLRFVRERLDGAGDAWVDGRATAGYQGAPVKR
                     NQQIAEHSPLAREIGDVILASIERNPLFISAVLPNQVYPPLFNRYAGGMEFGSHVDGA
                     VRVLPNGIKLRTDVSATLFISEPAEYDGGELVIEDTYGVQQVKLPAGDMIVYPATSLH
                     QVTPVTRGARLASFFWVQSLVRSDTQRALLFDLDTAIQRLNATHADDVARRSLVGCYH
                     NLLRTWSET"
     gene            complement(1729..>2760)
                     /locus_tag="VHV99_08775"
     CDS             complement(1729..>2760)
                     /locus_tag="VHV99_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006417007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX3635568.1"
                     /translation="KFDTGPIKHDLITGIDLGHDSYSNQAYTRNNLPIVSMVNPQYLA
                     NPAVVTTTVGNYADSGSNEIGAYVNDTVSLGRDWKFIGGLRWDRFQAQIHNTISLPGY
                     ATQTNFFTSVRAGVIYQPTDWQSYYVSYGTSFNPSLEALTVTNLTQNLAPESTRSYEV
                     GAKWDLLGGNLSVTSAFFREEKTNARTQVSPTEYELDGDIRVDGFQAGVTGHITDKWQ
                     IFGGYTYMDAIILKALDGTQGHVPANTPRNTLTLWSTYAITPHWEIGGGPTYMSPRYA
                     SNTNYVQVPGYTRWDATAAYHAKQYDIRLNLLNLTNKTYYDALIPSDGGRSVPGIGRT
                     LLATFDYRF"
BASE COUNT          505 a          849 c          869 g          537 t
ORIGIN      
        1 cggcaagaaa cgacggcgtg acgaggttcg cgggcgtgcc ctcctccgac atgaagctgt
       61 ccggcttttt caacgccatc tcctcaacgc tgctgtttcg aattcgtgcg gaccggaata
      121 gcaaaaacga ggccagacgc gtcggacggc tgcccggagg cagccatcga cgtagcgcga
      181 ttctcgcacg gagcggcgtt ttggaggaag ctagggtgac gcgcgaaaga cctgaaacga
      241 aagggttttt agagcaaccc catgcagaga gccgcgacga tcgatcccgt caccagcatg
      301 gcgccgatgg ttttgcgttt gttcagctcc gtccacagca ggacgccagt gagcgacagc
      361 aaaatgatgc tgcccgccag cgtatcgatc agcagcaccc agccgacgct caagccaacg
      421 cccttgtgca gattcgtgag catcgcgaga aacgagtttt cgctgcgctt gactgtcacg
      481 taaccattgc cgacccaata ctcagcggcc gtgttttcat gcggcgccgc gaagttgagc
      541 tgccagtgtt ccggttggac catggtgcgg tcgccccacg cgaccggacg cgcgggctct
      601 ttctgcgcgc ggcccgggtt gccggcgagt ttgagttcat gcttgagcca cctggcgagg
      661 tcgcgcggcg tctgcggcgc cggttgcggc aacggcacct gcacgaccga cacccgcggc
      721 tcgccggtcg acactttgag cggtccgcca cgatgattca gcagaaagcc cgtggtgccg
      781 aacagcaaac ccagcgccgc cccccacaaa ccgatccagc catgcacttt gcgcagccac
      841 ttgacgaagg tagcgcggcg cgaacgctgc tgccgcgctt tcagctcccc ttggtcgagc
      901 cgcggcggtt cgctcgcttt ggcgtcgatg tccgcagccg gttggaattc aatagcctcg
      961 ggtgcgctca cgtttcgctc caggtacgca acaggttgtg atagcagccg accaggctgc
     1021 ggcgcgccac atcgtcggca tgcgtggcgt tcagacgttg aatcgccgtg tccagatcga
     1081 atagcaacgc gcgttgtgtg tcgctgcgca cgaggctttg cacccagaag aagctggcga
     1141 ggcgcgcgcc gcgtgtcacc ggcgtgacct gatgcagact ggtggcgggg tagacgatca
     1201 tgtcgcccgc cggcagcttg acctgctgca cgccgtaggt gtcttcgatc acgagttcgc
     1261 cgccgtcgta ttcggcgggt tcggaaatga acagcgtcgc cgagacgtcc gtgcgcagct
     1321 tgatgccgtt aggcagcaca cgcacggcac cgtcgacgtg actgccgaat tccatgccgc
     1381 ctgcgtaacg attgaagagc ggcgggtaca cctggttcgg caacaccgcg ctgatgaata
     1441 acgggttgcg ctcgatcgac gcgagaatca cgtcgccgat ctcgcgcgca agcggcgaat
     1501 gctcggcgat ctgctgattg cgtttgaccg gcgcgccctg atagccggcg gtcgcgcggc
     1561 catccaccca cgcgtcgccg gcgccgtcga gccgttcgcg cacgaagcgc aactgctcgg
     1621 cgttcagtac attcggaatg tgcagcagca tgttgggttt cctttgcctc gttacgccgg
     1681 cttgccgtgt gtggacacgg caagcctacc gtatcaatga gacacgcttc agaaccggta
     1741 gtcgaaggtt gccagcagcg tacgtccgat acccggcacg gaacggccac cgtccgacgg
     1801 aatcagcgcg tcgtagtacg tcttgttggt caggttcaac aggttcagac ggatgtcgta
     1861 ttgcttcgcg tgatacgcag cggtcgcgtc ccagcgcgtg tagcccggca cctgcacata
     1921 gttcgtgttc gacgcataac gcggcgacat ataggtcggg ccgccgccga tttcccaatg
     1981 cggcgtgatc gcataagtac tccagagcgt cagcgtgttg cgcggcgtgt tggctggcac
     2041 gtgcccttgc gtgccgtcga gcgccttcag gatgatggcg tccatgtacg tgtagccgcc
     2101 aaaaatctgc cacttgtcgg tgatatgacc ggtcacgccg gcctggaagc cgtcgacgcg
     2161 gatgtcgccg tcaagctcgt attcggtcgg cgaaacctgc gtgcgggcgt tggtcttttc
     2221 ctcgcggaag aacgccgacg taaccgaaag attgccgccg agcagatccc atttcgcgcc
     2281 gacttcatac gacctggtcg attcgggcgc gaggttctgc gtcaggttcg tcaccgtcag
     2341 cgcttcgagc gaaggattga acgacgtccc gtacgagacg taatacgact gccagtcggt
     2401 cggctgatag atcacaccgg cgcgcacgct ggtgaagaag ttggtttgcg tcgcgtagcc
     2461 cggcaggctg atcgtgttgt ggatctgtgc ctggaaacga tcccagcgca ggccgccaat
     2521 aaacttccag tcgcggccca gcgagacggt gtcgttgacg tacgcgccaa tctcgttcga
     2581 acccgagtcc gcgtaattgc cgaccgtcgt cgtcaccacc gcgggattcg ccaggtactg
     2641 cggattcacc atcgaaacga tcggcaggtt gttacgcgtg tacgcctggt tggaatagct
     2701 gtcgtgcccg agatcgatac cggtgatcag gtcgtgcttg atcggaccgg tgtcgaactt
//
LOCUS       DATASH010000311         5604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2021638,
            whole genome shotgun sequence.
ACCESSION   DATASH010000311 DATASH010000000
VERSION     DATASH010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5604
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2021638"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..99
                     /locus_tag="VHV99_08780"
     CDS             <1..99
                     /locus_tag="VHV99_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018432068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEX3635569.1"
                     /translation="QVLTLVERLGPAQWRESRLDRVFFVPLKSGVI"
     gene            105..1040
                     /locus_tag="VHV99_08785"
     CDS             105..1040
                     /locus_tag="VHV99_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX3635570.1"
                     /translation="MSMLRAMQRTRLTIPMTVTQRSVCVLALSLLMSACATRLDQAPV
                     VDRSGSGALGTQAAQQPAVPLGPTPPGYYRVKPGDTLYRIALENGQNYRDISTWNNLT
                     NPNQIEVDQLLRVVPPGANTAALTPGVSTAPIGNGGVVQSAPLGSTPPATGGAAGVAA
                     PPIYGSTANNAASTPPAAGAASDSTAGASGNVAFAWPVRGPMLGTFNDSTNKGINIGG
                     AAGDTVKASADGRVVYAGNVLRGYGNLIIIKHDATYLTAYAHNRALMVKEGDAVTKGQ
                     KIAEMGNSDSDRVMLHFEVRRQGKPVDPLKYLPPQ"
     gene            1052..2134
                     /gene="rpoS"
                     /locus_tag="VHV99_08790"
     CDS             1052..2134
                     /gene="rpoS"
                     /locus_tag="VHV99_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004193640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HEX3635571.1"
                     /translation="MPKSKRRLPQAESETISRVTSASVEESGASEVEEEIAEERDLDE
                     RQGSAEEAGEAREGASEAPPDADDFRALLQAELTADTIQHYLNRISVKPLLTVEEEQK
                     YSRLAKAGEFDARQVMIERNLRLVVSIAKGYLNRGVPLLDLIEDGNLGLMHAIEKFDP
                     TRGFRFSTYATWWIRQSIERAIMNQARTVRLPVHVIRELNQVLRAKRHLEKNSMNSGE
                     AAERRDASIDDIAYLTGKTTDEVTDILALNEHTASLDAPLDLDPASSLLDLLSDDQSQ
                     SPDAEVQHRELETLTRAWLARLSDKHRHVIERRFGLNHIEPATLEELADEMGLTRERV
                     RQIQQEALVRLKRFFASNGVRKDAVL"
     gene            2139..2915
                     /locus_tag="VHV99_08795"
     CDS             2139..2915
                     /locus_tag="VHV99_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX3635572.1"
                     /translation="MTPILVFDIETIPDVAGIRRLEDLPATLSDAEVAEHAFAARREK
                     TGGDFLPHHLQRIAAISCVFRDNNGFRVRSLGTLEDGEAALVQSFYRVIEKYTPQLVS
                     WNGGGFDLPVLNYRALVNGIPAYRFWDLGEDDREFKWNNYISRYHARHTDLMDVLAMY
                     QARANAPLDALAKMCGFPGKMGMDGSQVWHAYQQGRIEEIRNYCETDVVNTYLLYCRF
                     QLIRGAFSPEEYADEINLVKNALALETAPQWAEYLAAFDQ"
     gene            3149..4567
                     /gene="rlmD"
                     /locus_tag="VHV99_08800"
     CDS             3149..4567
                     /gene="rlmD"
                     /locus_tag="VHV99_08800"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008924102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEX3635573.1"
                     /translation="MSRTAPQRRSPKRQKAPAPAKAHVITGAEPIIEIVSLDMEARGV
                     GRIESEDGTPGKVIFVEGALPGERVSYSTHRSKPKFEQAQVVQVFRESVLRTRPKCIY
                     FDICGGCSMQHLDIRAQIAVKQRVLEDNFQHLAKLRPETVYRPIHGPAWGYRYRARLA
                     VRYLPEKGGMRIGFYEKNSSYISDMKTCEVLPPHISAMLMPLRFMVRKLSIYDRTPQI
                     ELAVGSSVTALVLRNLAPITAADEQVLRDFADEHKIQWWLQPGGPDTVTPFYPLDVQL
                     DYTLPEFNIRMPFKPTDFTQVNHAINRVLVSRALRLLAPVRTDRVLDLFCGIGNFTLP
                     LARISKEVVGIEGSEVLTSRALANAELNGVAGHTSFACRNLFEVTADDMRALGHFDKF
                     LIDPPREGALAVAKALAEIAQSGNGPLPRRIVYVSCNPATLARDSGMLVHEAGYRLVG
                     AGVVNMFPHTSHVESIALFERD"
     gene            complement(4689..5549)
                     /locus_tag="VHV99_08805"
     CDS             complement(4689..5549)
                     /locus_tag="VHV99_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006478696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEX3635574.1"
                     /translation="MRALNTITKAAINLAAIKKAARKVAGYIACAAAVAVVAACHGLP
                     EKTDETATWNNNKLYTEANDALTGGDFGKCAKYFEMLEGRDPFGHFAQQAQINVAYCN
                     WKDNENAAADQAIDRFIQLHPDHPDIAYAYYLKGMIHFNDDLGLFGRFSGQDMSERDP
                     VSLRQSYDAFKIVVDKYPNSKYAPDAAQRMRYIVNALASHEVHAADYYYRRGAYVAAI
                     NRAQLALKEYKNARAIEDALHIMMLSYERLNQPQLADDTKRILTGTFPDSPYITGHAR
                     PGKEKSWWQF"
BASE COUNT         1055 a         1719 c         1766 g         1064 t
ORIGIN      
        1 caggtgctga ctctggtcga gcgcctcgga cccgcgcagt ggcgcgagtc gcggcttgat
       61 cgcgttttct ttgtaccctt aaaatccgga gtgatttgac accgatgagt atgttgcgcg
      121 cgatgcaaag aaccagactg actatcccca tgaccgtaac ccagcgtagc gtgtgcgtgc
      181 tcgccttgtc cctgttgatg tcggcttgtg cgacccgcct cgatcaggcg ccggtcgtcg
      241 accgctccgg cagcggtgcg ctcggcacgc aagccgcgca gcagccggcc gtgccgctcg
      301 gaccgacgcc gccgggctac taccgcgtga agccgggcga tacgctgtac cggatcgcgt
      361 tggagaacgg ccagaactat cgcgatattt cgacgtggaa caatctcacc aacccgaatc
      421 agatcgaagt cgatcagttg ctgcgcgtcg tgccgccggg cgcgaacacc gccgcactga
      481 cgccgggcgt gtcgacggcg ccgatcggca acggcggcgt ggtgcagagc gcgccgctgg
      541 gtagcacgcc accggcaacg ggcggcgcgg ctggcgtcgc ggcgccgccg atctacggct
      601 ccacggcgaa caacgccgcg tcgacgccgc cggcagcggg cgccgccagc gattcgaccg
      661 ccggcgcctc gggcaacgtc gccttcgcat ggccggtgcg cgggccgatg ctcggcacgt
      721 tcaacgattc gaccaacaag ggcatcaata tcggcggcgc ggccggcgat accgtcaagg
      781 cttcggcgga tgggcgcgtg gtttatgccg gaaatgtgct gcgtggttac ggcaatctaa
      841 ttatcatcaa acatgatgca acttatctca ccgcgtatgc acacaaccgc gctttgatgg
      901 taaaagaggg ggacgcggtg accaaagggc agaagatcgc tgagatgggc aatagtgatt
      961 ccgaccgcgt aatgttgcat ttcgaagttc gccggcaggg taaacctgtc gacccactga
     1021 agtatttgcc gccgcaataa gcgatacgac catgccgaaa tcgaagcgcc gcctgccgca
     1081 agccgagtct gagacgatta gccgtgtgac gtccgcttcg gtggaggaaa gcggcgcttc
     1141 ggaagtagaa gaagagatcg cggaggagcg cgatctcgac gaacgccagg gcagcgcgga
     1201 agaggccggc gaggcccgcg aaggcgcgag cgaagccccg cccgacgcgg acgatttccg
     1261 cgcgctgctg caagccgagc tgacggccga cacgatccag cattacctga accgcatcag
     1321 cgtcaaaccg cttctgaccg tcgaagaaga gcagaagtat tcgcgtctgg cgaaggcggg
     1381 cgagttcgac gcgcggcaag tgatgatcga gcgcaattta cggctcgtcg tgagcatcgc
     1441 aaagggttac ctgaatcgcg gcgtgccgct gctcgatctg atcgaagatg gcaacctcgg
     1501 actgatgcac gccatcgaga agttcgatcc cacacgcggc ttccgtttct ccacctatgc
     1561 cacctggtgg attcgccaaa gcatcgagcg cgcaatcatg aaccaggcgc gcacggtgcg
     1621 tttgccggtg cacgtgatcc gtgagctcaa tcaggtgctg cgcgccaagc gccacctgga
     1681 aaagaattcg atgaattccg gcgaggcagc cgagcgccgc gacgccagca ttgacgacat
     1741 tgcctatctg actggcaaga ccaccgacga agtcactgac attctggcgc tgaacgagca
     1801 caccgcgtcg ctcgacgcgc cgctcgatct cgacccggcc agcagcctgc tcgatctgct
     1861 ctccgacgac caaagccagt cgccggatgc cgaggtgcag caccgcgaac ttgaaacgct
     1921 cacgcgagcg tggctcgcgc gcctgtcgga taagcatcgt catgtgatcg aacgccgctt
     1981 cgggctgaac cacatcgagc ccgccacgct tgaagaactg gccgacgaaa tgggcctcac
     2041 gcgcgagcgc gtgcgccaga tccaacagga agcgctggtg cggctcaagc gtttctttgc
     2101 ctccaacggt gtgcgcaagg acgccgttct ataacctgat gacaccgatt cttgttttcg
     2161 acatcgagac gattcccgat gtcgccggca ttcgccgcct cgaagatttg cccgccacgc
     2221 tgagcgacgc cgaagtcgcc gagcacgcct ttgcggcgcg ccgcgaaaag accggcggcg
     2281 atttcctgcc gcatcacctg cagcggatcg ccgcgatttc ctgcgtgttc cgtgacaaca
     2341 acggtttccg ggtgcgctcg ctcggcacgc tcgaagacgg cgaggcggcg ctcgtgcagt
     2401 cgttttatcg cgtgatcgag aagtacacac cgcagctggt gtcgtggaac ggcggtgggt
     2461 tcgacctgcc ggtgctgaac tatcgcgcgc tcgtcaatgg catcccggct tatcgtttct
     2521 gggacttggg cgaggacgac cgcgagttca agtggaacaa ctacattagc cgctatcacg
     2581 cgcgtcacac cgatctgatg gacgtgctgg cgatgtatca ggcgcgcgcc aatgcgccgc
     2641 tcgatgcgct cgcgaagatg tgcggcttcc ccggcaagat gggcatggac ggcagccagg
     2701 tgtggcatgc ataccagcaa gggcgcatcg aggaaattcg caattattgc gagaccgacg
     2761 tcgtcaatac gtacctgctg tattgccgct tccagctgat tcgcggcgcg ttttcgcccg
     2821 aagagtatgc ggacgagatc aatctggtca aaaatgcgct tgcgctggaa acggcgccgc
     2881 aatgggctga gtatctggcc gcgttcgacc agtagcctgc ttacctgtct tgtaacgaaa
     2941 tggtttatcg cgcgctgatg gcgtagacgc tgcgatggcc gccgccgtag tgcgaaatcg
     3001 cgcgcaagtg ccgcggttcg cgttacgctc atcatggtcg acgtgctccg cgcggcgcgg
     3061 cggccactag cgccgcgccg aatccttggc aagggcggcg cttcgtctac aatctcgcct
     3121 ttcccccaag tttgtcagga agtcgcaggt gtcccgaact gccccccaac gccgctcgcc
     3181 gaagcgccag aaggcgcccg ctccggcgaa agcgcacgtc atcaccggcg ctgagccgat
     3241 catcgaaatc gtttcgctcg acatggaagc gcgcggcgtg ggccgcatcg agagcgaaga
     3301 cggcacgccc ggcaaggtga tttttgtcga aggcgcgttg ccaggcgagc gtgtgagcta
     3361 ttcgacgcat cgcagcaagc cgaaattcga gcaggcgcag gtcgtccagg tgttccgcga
     3421 aagcgtgctt cgcaccaggc cgaaatgcat ttacttcgat atctgcggcg gttgttcgat
     3481 gcagcatctc gatatccgcg cccagattgc cgtcaagcag cgcgtgctcg aagacaattt
     3541 ccagcatctg gcgaagctgc gtccggagac ggtgtaccgg ccgattcacg ggccggcgtg
     3601 gggataccgc taccgcgcgc gtctggcggt gcgctatctg ccggaaaagg gcggcatgcg
     3661 gatcggcttc tacgagaaga acagcagcta catctccgac atgaagacct gcgaggtgct
     3721 gccgccgcat atttcggcga tgctaatgcc gttgcggttc atggtccgca agctgtcgat
     3781 ttacgatcgt acgccgcaga tcgagcttgc ggtcgggtcg tcggtcaccg cgctggtgct
     3841 gcgcaatctc gcgccgatca ccgccgccga cgaacaggtg ctgcgcgatt tcgccgacga
     3901 acacaagatc cagtggtggt tgcagcctgg cggtcccgac acggtcacgc cgttctatcc
     3961 gctcgacgtg cagctcgact acacgctgcc ggaattcaac atccggatgc cgttcaagcc
     4021 gaccgatttc acccaggtga atcatgcgat caatcgcgtg ctggtgagcc gcgcgctgcg
     4081 tctgttggcc ccagtccgta cggaccgcgt gctcgatctg ttctgcggca tcggcaattt
     4141 cacgctgccg ctggcgcgga tttcgaagga agtggtgggc atcgaaggca gcgaggtgct
     4201 gacatcgcgc gcgctggcca atgccgaact gaacggcgtg gcgggccata cgtcgttcgc
     4261 ctgccgcaac ctgttcgaag tcaccgccga cgacatgcgc gcgctcggtc atttcgacaa
     4321 gtttctcatc gacccgccgc gcgaaggcgc gctggcggtc gccaaggcac tcgcggagat
     4381 cgcgcagagc ggcaatgggc cgctgcccag gcggatcgtc tacgtgtcgt gcaacccggc
     4441 gacgctcgca cgcgattcag gcatgctggt gcacgaggcc ggctaccggc tggtgggcgc
     4501 gggggtggtg aacatgttcc cgcacacttc gcacgtggag tcgattgcgt tgttcgagcg
     4561 ggactgaggg cgtcggttgc cagcttgtgc tgcataggtt ggcggcgcgc agcacaaaca
     4621 aaaacgccac gaccgaggtc gtggcgtttt tgttttaaag ctttatgttg aaagcgttcg
     4681 gccgaggctt agaactgcca ccacgacttt tccttacccg gccttgcatg gcccgtgatg
     4741 tacgggctgt cggggaaggt accggtcaga atgcgcttcg tgtcgtcggc cagttgcggc
     4801 tgattcagcc tctcgtacga cagcatcatg atgtgcagtg cgtcttcgat agcccgcgcg
     4861 ttcttgtatt ccttcaacgc cagctgcgcg cggttgatcg cggcgacgta ggcaccacgg
     4921 cggtagtaat aatccgccgc atggacttcg tgcgacgcca gcgcgttcac gatatagcgc
     4981 atgcgttgcg cggcgtccgg cgcatacttg ctgttcgggt acttgtcgac cacgatcttg
     5041 aacgcgtcat acgactggcg cagcgacacc ggatcgcgct cgctcatgtc ctggccggag
     5101 aagcggccga acagaccgag gtcgtcattg aagtggatca tgcccttcag gtagtacgcg
     5161 taggcgatgt ccgggtgatc cgggtgcaac tggatgaagc ggtcgatcgc ctggtcggcg
     5221 gcggcgtttt cgttatcttt ccagttgcaa tacgcgacgt tgatctgcgc ttgctgggca
     5281 aagtggccga acgggtcgcg gccttcgagc atctcgaaat acttggcgca cttgccgaaa
     5341 tcgccgccgg tcagagcgtc gttcgcctcc gtatataatt tgttgttgtt ccacgtagcc
     5401 gtctcgtcgg tcttttccgg caggccgtga caagccgcaa caacggcgac ggccgcggcg
     5461 caggcaatgt atccggccac tttccgggcc gccttcttga tggccgccaa attgatcgcc
     5521 gctttagtga tggtgttcaa ggctcgcatt ttccagtcta gctttacgtc caggtgaccc
     5581 agactcaatg acccgctcaa atac
//
LOCUS       DATASH010000312         2768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1823809,
            whole genome shotgun sequence.
ACCESSION   DATASH010000312 DATASH010000000
VERSION     DATASH010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1823809"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..2188
                     /locus_tag="VHV99_08810"
     CDS             35..2188
                     /locus_tag="VHV99_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3635575.1"
                     /translation="MQRAERIAVDVPMPLRNFAATRRVLLIVLAVSIVFPLACLAGYG
                     YFDYQRRIADSNDMIDRLARVTEEQAVKVLDLNQQMASRIVDLLGNDADARIREQQAG
                     LHDRLRQIGGDFPQVASIYLLGANGDLLVSSLSYPAPVMSTRLREDFITAKAMRPQPY
                     FSQAMFGALSQTNVFNTATGRSGENGQFLGVVSVALRTEYFSRFYRELINGDTSLALG
                     LYRQDGSLLVRYPAWVAGAQPTPQSAFTAALRDQQLFGHIRLKSTVDGVERLLAFRRV
                     GDYPLYVMSAYETASIGSAWRQHFMMIAALTAVPCVAIWLLVFFSLRQLAGERRAWER
                     WQGEVAMRLSAEASSRQLQRMGALGNLVANVAHDFNNLLMVVSANTELARLKRYNNLQ
                     KEVQAVERATATAESLTRRLLSVAKKQPLKQEAVDLATWLPSAAPLIDAALGDNVEMT
                     LSMVDNLWQVLADPIDLEFAIMNLAVNARDAMPRGGRFVIRCQNNRLVGSDTWLPDGE
                     YVLIACSDDGEGMPEAVARRAFEPLFTTKLRGLGTGLGLAQVLAMCEQAGGMAKIDSV
                     PGSGTTVRLYLPRYREREKASAPDAEAAQLPAPMTKGVVLLVEDNEDVAAGVAAVLET
                     FGCEVRHEPTADQALDVLTGGAQFELVLSDIQMPGKLNGIDLAEKVRSAWPAQKIALM
                     TGYADELERARRLGVAILAKPFNIDELHALVVCQP"
     gene            2405..2686
                     /locus_tag="VHV99_08815"
     CDS             2405..2686
                     /locus_tag="VHV99_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635576.1"
                     /translation="MKIRNALLVSTLLAASSLAMAQGSGGGGGGGPAGNDPNAGTNAG
                     ATREGGDMQASGAMAHKSTKQHHMKHTKKPAADMTSTPGADASSATKGQ"
BASE COUNT          497 a          881 c          905 g          485 t
ORIGIN      
        1 tgacctgtat ttacctttca tggagatcgt gaccgtgcag cgtgcggagc gtatcgcagt
       61 cgatgtcccc atgccgttgc gtaatttcgc ggcgacgcgg cgcgtgctgc tgatcgtgct
      121 tgccgtttca atcgtgttcc cccttgcgtg tctcgcgggt tacggctatt tcgattacca
      181 gcgccgtatc gccgattcga acgacatgat cgatcggctc gcgcgcgtga ccgaagaaca
      241 ggccgtcaag gtgctggacc tcaaccagca aatggcctcg cgtatcgtcg atctgctcgg
      301 taatgacgcc gacgcgcgga ttcgcgagca gcaagccggc ctgcacgatc gtctgcggca
      361 aatcggcggc gactttccgc aagtggcgtc gatttacctg ctcggcgcga acggcgatct
      421 gctggtgtcg agcctgagtt atccggcgcc ggtcatgtcg acccgccttc gcgaagactt
      481 catcaccgcg aaggcgatgc ggccacagcc gtatttttct caggccatgt tcggggcgct
      541 gtcgcaaacc aatgtattca acaccgcgac cggccgctcc ggcgagaatg ggcaattcct
      601 cggcgtggtg tccgtggcgc tgcgcaccga gtacttttcg cgcttctacc gcgagttgat
      661 caacggcgat acgtcgctcg cgttgggact gtatcgtcag gacggcagtc tgctggtgcg
      721 ctatccggct tgggtcgcgg gcgcgcaacc caccccgcag agcgcattca ccgcggcctt
      781 gcgcgaccaa cagctgttcg gtcacatccg cctgaagtcc acggtcgacg gtgtcgaacg
      841 gctgctggcg tttcgccgcg tcggcgacta tccgctctat gtgatgagcg cgtatgaaac
      901 cgcctcgatc ggcagcgcgt ggcgccagca tttcatgatg atcgcggcgc tcacggccgt
      961 gccctgcgtg gcgatctggc tgctggtgtt tttctcgctg cgccagctcg ccggcgagcg
     1021 ccgcgcgtgg gaacgctggc agggcgaagt ggccatgcgt ctttccgcgg aggcgtcgag
     1081 ccggcaattg cagcgtatgg gcgcgcttgg caatctcgtc gccaatgtcg cgcacgactt
     1141 caataatctg ctgatggtgg tgtcggctaa caccgaactc gcacggctca agcgctataa
     1201 caatctgcaa aaagaagtgc aggccgtcga gcgcgccacg gccacggcgg aatcgctgac
     1261 gcggcggctc ctgagtgtgg cgaagaagca gccgctcaag caggaagcgg tcgacctcgc
     1321 cacatggctg ccgagcgccg caccgctcat cgacgcagcg ctcggcgaca acgtcgaaat
     1381 gaccttgagc atggtcgaca acctctggca ggtgctggcc gatccgatcg acctcgaatt
     1441 cgcgatcatg aatctggcgg tcaacgcgcg cgatgcgatg ccgcgcggcg gccgcttcgt
     1501 gattcgttgc cagaacaacc gtctggtggg cagcgacacc tggctgcccg acggcgaata
     1561 tgtgctgatc gcctgttccg acgacggcga agggatgccc gaagcggtcg cccgtcgcgc
     1621 cttcgagccg ctcttcacca ccaagctgcg cggcttgggc accggcctcg gcctcgccca
     1681 ggttctcgcc atgtgcgagc aggcaggcgg catggccaaa atcgacagcg tgccgggcag
     1741 cggcacgacg gtcagacttt acttgccgcg ttatcgcgag cgcgagaagg cgtcggcgcc
     1801 cgacgccgaa gcggcgcagc tgccggcgcc gatgacaaag ggcgtcgtgc tgctggtgga
     1861 agacaacgaa gacgtcgcgg cgggcgtagc cgccgtgctg gaaaccttcg gctgcgaagt
     1921 gcggcacgaa ccgaccgccg accaggcgct tgacgtgctg accggtggcg cgcagttcga
     1981 actcgtgctg tccgacatcc agatgccggg caagctcaac ggcatcgacc tcgcggagaa
     2041 agtgcgcagc gcgtggcccg cgcagaagat cgcgctgatg acgggctatg ccgacgagct
     2101 cgaacgggcg cgccgcctcg gcgtggcgat actcgccaag cccttcaaca tcgacgaact
     2161 gcacgcgctg gtggtctgcc aaccgtaggg cttgcagcat cgtcggcatg cccggcggcg
     2221 cgatcagggc gccgattact ttcgacggca cgcagcgggt ctgacggccc cacctctgcg
     2281 cttccagccg tgctcgaagc gtttcatgac tcgcggcaca ggccttgctg acaagatgac
     2341 gaggatgcgt atccggctgg accagccggc gcaacccact tctttttcgg ccaggagaat
     2401 acccatgaag atcagaaacg cattgctcgt ctcgacactg ctcgccgcgt cgtcgctcgc
     2461 gatggcccaa ggcagcggcg gcgggggcgg gggcggcccg gcaggcaatg acccgaatgc
     2521 aggcaccaac gccggggcga cgcgcgaagg cggcgacatg caggcttccg gtgcgatggc
     2581 gcataagtcg acgaagcaac accacatgaa gcacacgaag aagccggccg ccgatatgac
     2641 cagtacgccg ggcgccgacg ccagcagcgc tacgaaggga cagtagggag gaagtgggct
     2701 tcgcatgacc gcacattgtg cgaagttgct ttggctttga gcccgaaggc ctgcaggctc
     2761 ataagcca
//
LOCUS       DATASH010000313         4515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6571253,
            whole genome shotgun sequence.
ACCESSION   DATASH010000313 DATASH010000000
VERSION     DATASH010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4515
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6571253"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="VHV99_08820"
     CDS             <1..521
                     /locus_tag="VHV99_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX3635577.1"
                     /translation="ETISHAIEESGISARRLELEITETAMMEYPELVSEKIFALKRLG
                     VRIALDDFGTGYSSLSYLNRFSVDTLKVDRSFIQAIPGDRSVCVMVTAIVNLARSLGL
                     TVVVEGTETEEQIAWLAALGHIEAQGFLFSRPVPVDAIPALLERFGVCGTKGHHPAHL
                     ADSTTSVSSTNS"
     gene            736..1944
                     /locus_tag="VHV99_08825"
     CDS             736..1944
                     /locus_tag="VHV99_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEX3635578.1"
                     /translation="MQTITSQNAARRGVQEPLWTLFRVVFGLSALSWGGLALMAQLEN
                     HYVEREERLSRVAFADLIALAWMVPGPVGCNVAVQLGHALRGRAGAWVAGIASVLPFF
                     MLMTLFAIFYRTPLVRAAASQTLLNHFSVVLATLIAITWYKQTRALVRGKLEWIAAVL
                     GCVALFYARSPAAYVLMLGAAFGAGWLVSPVRNARIVVSIGRGDWHMLAALCGLLVLF
                     ALPLPHRYELALLWPRLAGAGMTLFGGGFSALPVLKTLFVTPAIGVSDNDFTLAFSLS
                     PLSPGPLLNVVPFFGYLVDGWTGALIATLALFVPSGCLVVLAQRHLHQLKANPRFEHG
                     MRILRAVTTAFLAVAVLRIAAHVPFKPVYLVTALFSAMCFAKLKVPVYAVYGTVAVVC
                     GLWLAYGAIP"
     gene            complement(2038..2496)
                     /locus_tag="VHV99_08830"
     CDS             complement(2038..2496)
                     /locus_tag="VHV99_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006413474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEX3635579.1"
                     /translation="MNKAIELALFLHLLAVAVWVGGMIFAHFCLRPAIADLSPQLRLP
                     LWESVFGRFFNWVAAAVLVILLTGGFLLMQFGGGHATWPLHAMAGLGIVMMLIFGHIR
                     FAVFPRIRRAVQAQKWPDGAQAVGTIRRLVLVNIVLGVVTIGVAVLSRGF"
     gene            complement(2845..>4515)
                     /locus_tag="VHV99_08835"
     CDS             complement(2845..>4515)
                     /locus_tag="VHV99_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX3635580.1"
                     /translation="MQHIAGPDFPGGGQIISSDAEISAAYESGRGSLKVRARWKIEEL
                     ARGQWQLVITELPPNTAAQKVLEEIEEQTNPKIKLGKKALTPEQLQTKQTMLALLDAV
                     RDESGRDSPVRLVFEPKSSRIDQTDFVNTLLAHTSLESNASLNMVMVGGDGRPRQKGL
                     SEIIHEWIAFRFATVTRRTRHRLTKVDDRIHILEGRMIVFLNIDEVIRIIRESDEPKV
                     ALMAAFSLSDRQAEDILEIRLRQLARLEKIKIEKELSELRDEKAKLEELLGNESAMKR
                     LLIKEIEGDAKQYGDERRTLIQQEKRATFEARVVDEPVTVVVSQKGWVRALKGHGLDV
                     AGFTFKAGDGLYAAFQCRTPDTLVAWGSNGRVYSVAVAILPGGRGDGVPVTSLIELES
                     GSHLMHYYAASADQALLLASSNGFGFVAKVGDMISRVKAGKSFMTIDAGAAPLAPMPM
                     LPDATQIACLSSGGRLLVFGLDEMKTLSGGGRGVTLMALDDNETLVQALSINKAGVVL
                     IGTRRGGRVDEEKLSGATLTPHVGKRARKGRAPESKMKLDSMRPVLAA"
BASE COUNT          765 a         1452 c         1377 g          921 t
ORIGIN      
        1 acgagaccat ctcgcacgcg atcgaagaaa gcggcatctc cgcgcgccgc ctcgaactcg
       61 aaatcaccga gacggcgatg atggaatatc ccgaactcgt ctcggagaag atcttcgcgt
      121 tgaagcgcct cggcgtgcgc atcgcgctcg acgacttcgg cacaggttat tcgtcgctct
      181 cttacctgaa ccgcttctcg gtcgacacgc tgaaggtgga ccgttcgttc atccaggcga
      241 ttccgggcga ccgtagcgtg tgcgtgatgg tcaccgcgat cgtcaacctg gcgcgttcac
      301 tcgggcttac ggtggtggtc gaaggcaccg agactgaaga gcagattgca tggctcgccg
      361 cgctcggcca tattgaagcg caggggttcc tgttctcgcg tccggtgccg gtggacgcga
      421 ttccggcgct cctcgaacgc ttcggcgtat gcggcacgaa ggggcatcat cccgcgcacc
      481 tcgccgacag caccactagc gtgtcgagca ccaatagctg aacgcgggtt cgacggtggc
      541 gcgtatggac ccgtgccacg ggcaaacgca ctagcctcta taagcggcga ctccgcctta
      601 tctaaccgca gcatcacaaa accggctaca tttcacgctg tactgcgata cggcgcgcgg
      661 ccggctgccg gccgcgccaa cccacaccga tgatccaaga gggcgggccg cccgcgtggc
      721 ggagcccgcg gcgccatgca gacgatcacg agccagaatg ccgccagacg aggagtacag
      781 gagcccctat ggacgctgtt ccgggtcgtg ttcggcctgt cggcgctgtc atggggcggc
      841 ctcgcgctga tggcgcaact tgaaaaccac tacgtggaac gcgaggaacg gctctcgcgc
      901 gtggcctttg ccgatctgat cgcgcttgcc tggatggtgc ccggaccggt gggctgcaat
      961 gtcgccgtgc aactcgggca tgcgctgcgc ggacgcgccg gcgcctgggt ggcgggcatt
     1021 gcaagcgtgc tgcccttttt catgctgatg acgctgttcg caatcttcta tcgcacgcca
     1081 ctcgtgcgcg ccgccgcttc tcaaacattg ctcaatcact tcagcgtggt gctggccacg
     1141 ctgatcgcga tcacctggta caaacagacg cgcgcgctgg tgcgcggcaa gctcgaatgg
     1201 atcgcggcgg tgctcggttg cgtggcgctc ttctatgcgc gcagtccggc ggcatacgtg
     1261 ctgatgcttg gcgcggcatt cggcgccggc tggctggtca gcccggttcg caatgcacgc
     1321 atcgtcgtgt cgatcgggcg cggcgactgg cacatgctgg ccgcgctgtg cgggttgctg
     1381 gtgttgttcg cgctgccgct gccgcatcgc tatgaactgg cgctgctatg gccgcgactg
     1441 gccggtgcgg gcatgacgtt gttcggtggc ggcttctccg cgttgccggt actcaagacg
     1501 ctgtttgtga cgcccgcgat cggtgtgtcc gataacgatt tcaccttggc gttttcgctt
     1561 tctccactgt caccgggacc gctgttgaat gtggtgccgt tcttcggcta cctggtggat
     1621 ggctggacag gcgcgttgat tgctacgctc gcgctgtttg tgccgtcggg ttgcctcgtg
     1681 gtactggcgc agcggcatct gcatcagttg aaggccaatc cgcgtttcga gcacggcatg
     1741 cggatcttgc gcgcggtcac taccgccttt cttgcggtgg cggtattgcg catcgccgcg
     1801 catgtgccgt tcaaaccagt ttatctggtg accgcgctgt tctccgcgat gtgcttcgcg
     1861 aagctcaagg taccggtgta tgcggtgtac gggaccgtgg cggtcgtgtg tgggttgtgg
     1921 cttgcctacg gcgcgatacc gtaggcggca ggggtccgcg cttgatagcg cggattccct
     1981 atgcgctcag cgttttcagc gcgcttcaga ctttcaatgc cgctaaaaag ccagctttca
     2041 gaatccgcgc gacagcacgg cgacaccgat cgtcaccacg cccagtacga tattcaccag
     2101 caccaggcgc cggatcgtgc cgactgcctg cgcgccgtcc ggccacttct gcgcctgaac
     2161 cgcacggcga atgcgcggga acacggcaaa gcgaatgtgc ccgaagatca gcatcatcac
     2221 gatgccgagg ccggccatgg cgtgcaacgg ccaggtggcg tgaccgccgc cgaattgcat
     2281 cagcaggaag ccaccggtga gaagaatgac gagcacagcg gcggcgaccc aattgaagaa
     2341 gcggccgaac accgattccc acaaaggcaa acgcagttgc ggcgaaagat cggcaatggc
     2401 cggccgcaga cagaaatgcg cgaatatcat cccacccacc cacaccgcga ctgcgagcag
     2461 atgtaaaaac agcgcgagtt cgatagcctt gttcatattt gtttttcctc tcctgacgac
     2521 gccacgccgc ttatgacgag tggtattaat agcggctatt gagcgcattc gaccgcgcac
     2581 catgcatgca gttccgtgcg ctcgtggcgt cttgacactc tttgtagttg ttgttgcagc
     2641 gcgcctgatg cgaagacggc gctgcacccc gattttctat gacgcacggc gcgtgcttca
     2701 tctgcgaatg atgcgcgccc gtgttgctac gctttcccgc ttcaggcagc ttccgtgttg
     2761 ctgcgctgtc ccgctcaggc aacttttgcg tcgctacact tccctgctgc aggcagcttt
     2821 caacagcttc gttatgcaag gccgttaagc cgccagcacc ggacgcatac tgtcgagctt
     2881 catcttgctc tccggcgcac ggcccttacg cgcccgcttg ccaacgtgcg gcgtgagtgt
     2941 ggcgccgctc agcttctctt cgtccacccg gccaccgcgg cgcgtgccga tcaacaccac
     3001 gcctgctttg ttgatggaca gcgcctgcac cagcgtttcg ttgtcgtcga gcgccatcag
     3061 cgtgacaccg cgtccgccgc ccgagagcgt tttcatctcg tcgaggccga acaccagcag
     3121 gcgtccacct gaagacagac acgcgatctg cgtcgcatcc ggcaacatcg gcatcggcgc
     3181 gagcggcgcg gcgcccgcgt cgatcgtcat gaacgacttg ccggccttga cgcggctgat
     3241 catgtcgccg accttggcga caaagccaaa gccgttgctc gatgcgagca gcaatgcctg
     3301 atccgcggaa gccgcatagt aatgcatcag atggctgccc gactcgagtt cgatcagcga
     3361 cgtaaccggc acgccatcgc cccgcccgcc cggcaggatc gcgaccgcca ccgaataaac
     3421 gcggccattg ctgccccacg caaccagcgt atccggcgtg cggcactgga atgcggcata
     3481 gaggccgtcg ccggccttga acgtgaagcc ggccacatcc aggccgtggc ccttcagcgc
     3541 gcgcacccag cccttctgcg acaccaccac cgtcaccggt tcgtcgacca cacgcgcttc
     3601 gaacgtggcg cgcttttcct gctggatcag cgtgcggcgc tcgtcgccgt attgcttcgc
     3661 atcgccttcg atttccttga tgagcagacg cttcatggcg gattcgttgc cgagcagttc
     3721 ttcgagcttg gccttctcgt cgcgcaactc ggacagttcc ttctcgatct tgatcttctc
     3781 gagccgcgcc aactgacgca agcggatttc gagaatgtct tcggcctggc gatcggagag
     3841 actgaacgcg gccatcaacg cgaccttcgg ttcgtccgat tcgcggatga tgcggatgac
     3901 ttcgtcgata ttcaaaaaga cgatcatccg gccttcgagg atatgaatcc ggtcgtcgac
     3961 cttcgtcaaa cgatgacgcg tgcggcgcgt aaccgttgcg aagcggaacg cgatccactc
     4021 gtggatgatt tcgctcaagc ccttctgacg cggccggcca tcgccgccca ccatcaccat
     4081 attgagcgac gcgttcgatt cgaggctcgt atgcgcgagc aacgtgttga cgaaatccgt
     4141 ctggtcgatg cggctcgact taggctcgaa caccagacgc accggtgaat cccggcccga
     4201 ttcgtcgcgc accgcgtcga gcagcgcgag catggtctgc ttggtctgca actgttcggg
     4261 cgtgagcgct ttcttgccga gcttgatttt cgggttggtc tgctcctcga tttcctcgag
     4321 caccttctgc gcggcggtgt tcggcggcag ttcggtgatc acgagttgcc actggccgcg
     4381 tgcgagttct tcgatcttcc agcgagcccg cactttcagg ctgccgcggc cgctttcgta
     4441 ggccgcggaa atctcggcgt cgctcgaaat gatctggccg ccgcccggga agtccggccc
     4501 ggcgatgtgc tgcat
//
LOCUS       DATASH010000314         2843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4397163,
            whole genome shotgun sequence.
ACCESSION   DATASH010000314 DATASH010000000
VERSION     DATASH010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4397163"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..689
                     /locus_tag="VHV99_08840"
                     /pseudo
     CDS             70..689
                     /locus_tag="VHV99_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611267.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(914..1075)
                     /locus_tag="VHV99_08845"
     CDS             complement(914..1075)
                     /locus_tag="VHV99_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635581.1"
                     /translation="MAEQPEDRTFEWRKFSKLAADGVKTGLFDFARIVSGIERGKCQK
                     GNLSGRLDP"
     gene            1362..1949
                     /locus_tag="VHV99_08850"
     CDS             1362..1949
                     /locus_tag="VHV99_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX3635582.1"
                     /translation="MRSTAVQPQRSLPHPTAGPRTKQDTAETGSGSQDALAVLEADRR
                     AVEKLFKAFGKTADDDLEARSALARRACEELSIHTMLEEELLYPAAQEALPDSDTIDV
                     EEAYVEHFLVKTLITRFETLKAGDKGFDATFKVMSEMVGHHVEEEEGELFPELRKSKC
                     NLQTLGEKTAKRKADLQSKLDAVGSRSVGEKSAML"
     gene            complement(2136..>2843)
                     /locus_tag="VHV99_08855"
     CDS             complement(2136..>2843)
                     /locus_tag="VHV99_08855"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013341537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX3635583.1"
                     /translation="GAINMLGLAKRVGAKIFQASTSEVYGDALVHPQKEDYWGHVNPI
                     GPRSCYDEGKRCAETLFMDYRRQHGLSVRIARIFNTYGPHMHPADGRVVSNFMMQALR
                     GEPLTLYGDGSQTRSFCYVDDMIDAFIGLMDVEEDPDGPVNLGNPHEITMREIAKRIV
                     TITGSASPIELHPLPADDPCHRRPDISRAYELLGWQPRTSLDEGLTATARYFFCTARR
                     GPAIAGSSCMRPHAAGA"
BASE COUNT          592 a          865 c          853 g          533 t
ORIGIN      
        1 tctcgagtcg gaatcgtaac cacggtcggc gtagatgacc ttcggcttgc gaagcggtcg
       61 accgccggga cctgctggag acggttgcgc gactggcaga aggcgggcgt atggaagcga
      121 ttgcacgagc tactgctggc gaagttacgc gaggccggcg aactggattt ctcgcgcgca
      181 gccgtcgact cttcatctgt gcgagccgtt ggggcgggcg aaaaactggt ccgaacccca
      241 cggatcgcgc gcgaccaggt tccaagcacc acattctcgt agacgcaaat ggcgttcctg
      301 tcagcgcgac cctgacaggc gccaatcgca acgacgtcac ccaactgctg ccgctggttg
      361 atgccattcc cccgattcgc ggcgtacgtg gccggccgct tcggaaaccc aaggtcatct
      421 acgcagaccg tggctatgac tccgaggcgc atcgccagaa acttcgcgag cgcggcatca
      481 agccggtgat tgccaagcgc cagacagaac acggcagtgg tctgggaaaa ttccgctggg
      541 ttgtcgaacg tactcatgcg tggcttcaca acttccgtcg tcttcgcatt cgcttcgaac
      601 gtcgggccga cattcacgaa gcatttctca aattcggttg ctccctcgtc tgctggaaca
      661 ttttcagacg gacggagcag tctttttgaa tccgtctcta aaggacgtcc ggcccatgat
      721 agacggctcg cacgctcata tcgctggcgt gaaggtatgc gctgagagca tccgccgacg
      781 tgcgcgaatc gtcgaccacc agcacgcgaa ctggcgcctc gttgtgaagg tgaccgttct
      841 gtatccggcc ggggcattcg agcgtttcag gttatctaga tcgggtgcat aggcctctcc
      901 agcagcggcg cttctacggg tcgaggcgac cggaaagatt ccccttctgg catttaccgc
      961 gttcgatacc gctgactata cgcgcgaaat caaagaggcc ggttttaacg ccgtctgccg
     1021 caagcttgct gaacttgcgc cactcgaatg tacgatcttc aggctgctcg gccatgaacc
     1081 cgtggatcgc attcagatcg tggatccgcg ttggaaccct taccgaccct atctgggcgc
     1141 gatgccgccg acaccagcgc agcaattctg gacgaccgtg gccaccttca gcgcttcgtc
     1201 tggcatggaa agaccctcaa cggcgccgcg cgtgctacga ctttccggga tcgcgacgaa
     1261 cgccaggtgc cacccccctc gtgtgtcgcc agttctcatc cgggttcttt gtcctgaatc
     1321 cgtgtaccgg ctgtctatcc gtaggcacac gagttgctga aattcgttca acggccgttc
     1381 aaccgcagcg gtcgttaccc catccgaccg cgggaccgcg gaccaaacag gacacggcag
     1441 agactggatc cggctcacag gacgcattag ccgtcctcga ggcagaccgc cgcgcagtgg
     1501 aaaaactatt caaggcgttc gggaagactg ccgacgacga cctcgaagcc aggtcggcgc
     1561 tcgcgcggcg cgcctgcgaa gagctatcga tccacacgat gcttgaagag gagctgcttt
     1621 atccggccgc gcaggaagcg ttgcccgaca gcgacacaat cgacgtcgag gaagcgtatg
     1681 tcgagcactt cctcgtcaag acgttgatca cacggtttga aacgctcaag gcgggcgaca
     1741 agggcttcga tgcgaccttc aaagtgatga gcgagatggt cggccatcac gtcgaggagg
     1801 aagaagggga actcttcccg gagctgcgca agtcgaaatg caatctgcaa acgctgggcg
     1861 aaaagacggc gaagcgcaaa gcggatttgc agagcaagct cgacgcagtc ggcagcagat
     1921 cggtgggcga aaagagcgcg atgctttaag tgcagcaacc cattagcaga tcgcgcatgc
     1981 caaccggaac caagcaacat gacgttagaa cggcggcttc gcagcatgct gcacgacttg
     2041 cgtggcatgt tcggcatggc acgactgcgc gtcggccagg gacgaacggt tgctgtgcga
     2101 ttcccaggcc actccaggcg taatgtcatg tcggactatg cgccggcggc gtggggacgc
     2161 atgcacgaac tcccggcaat cgccggcccg cgtcgagctg tgcaaaaaaa ataccgggcc
     2221 gtggcagtca agccttcgtc cagggaagtt cgcggttgcc agccgagcag ttcatacgcc
     2281 cgggagatat ccggccgacg atgacacgga tcgtcggcgg gcagcggatg caattcaatc
     2341 ggcgacgctg agccggtgat cgtgacgatg cgcttcgcga tctcgcgcat cgtgatctcg
     2401 tgaggattgc cgagattgac cggaccgtcc ggatcctcct cgacgtccat caggccgata
     2461 aaagcgtcga tcatgtcatc cacataacag aacgaacgcg tttgcgagcc atcgccgtac
     2521 aacgtgagcg gctcgccgcg cagcgcctgc atcatgaagt tcgacaccac gcggccgtca
     2581 gcgggatgca tgtgcggacc gtacgtattg aagatccggg caatccggac cgacagccca
     2641 tgctggcgcc ggtagtccat gaacagcgtc tcggcgcaac gcttgccttc gtcgtagcac
     2701 gaacgtggcc cgatcggatt gacatgtccc cagtagtctt ccttctgcgg atgcaccagc
     2761 gcgtcgccat acacttcgct tgtcgaagcc tggaaaattt ttgcccccac gcgtttggcg
     2821 agcccgagca tgttgatcgc gcc
//
LOCUS       DATASH010000315         6695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2221445,
            whole genome shotgun sequence.
ACCESSION   DATASH010000315 DATASH010000000
VERSION     DATASH010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6695
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2221445"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VHV99_08860"
     CDS             complement(<1..353)
                     /locus_tag="VHV99_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016354433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6232 family protein"
                     /protein_id="HEX3635584.1"
                     /translation="MQQDLPFNERGITFARAGLSAAGQLFPLRDLRGATVVMVPRQKP
                     LPVALGVIGLIVAVTGGILGSGPALLLGVMIAVVGYLSWITQDVIYRMYVTTPAGERE
                     VFTTKDEEFAQRVATL"
     gene            576..944
                     /locus_tag="VHV99_08865"
     CDS             576..944
                     /locus_tag="VHV99_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX3635585.1"
                     /translation="MKFLCLIFFDEHLLDDLSKRQYDALVDESLAYDERLRESGQFIA
                     AQALQSAKEAVTLRVRGEHVSITDGPFAETKEQVGGFLLIEARDMNEAMRVASGFPGA
                     RLGGVEVRPVKELRASWSDA"
     gene            971..1363
                     /locus_tag="VHV99_08870"
     CDS             971..1363
                     /locus_tag="VHV99_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006164282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3635586.1"
                     /translation="MSTLLKGSHAFSGFAVKDLAEAKAFYSNTLGLKVTSEPMGILKL
                     HLAGGNNVLVYPKPDHVPATYTVLNFPVDSVDRAVDTLSARGVNFEHYDMPGLKTDAK
                     GICRGSEGPVIAWFKDPAGNIFSVLEEQ"
     gene            complement(1394..2308)
                     /locus_tag="VHV99_08875"
     CDS             complement(1394..2308)
                     /locus_tag="VHV99_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3635587.1"
                     /translation="MDLKQIQYFIALFEDGSVTRAAKRLNIVQPALSMQIAKLEDELH
                     QQLFERGAHGMAPTAAGRLMYRLFLPIMRDLAHARQQLVQRDEVVTGHVSIGLIASVT
                     ESVLADSLSRFHARYPHVEVTVSDGYSATFIDWVSGGQLDAALINKPRSRLSLDSQPL
                     LDEEMVLVTSAVHGPDLPHVIELAQLPELELVLPTKRHGLRGVLDTAAQHEDVLLAPR
                     FEIDVLSTIVKLVESTRFATILPRIVVERAVRLGTLRVYPILAPRIVRHIVRISHPRR
                     PLSAAAEALIAIIADELRLVSSESTMQA"
     gene            2596..4062
                     /locus_tag="VHV99_08880"
     CDS             2596..4062
                     /locus_tag="VHV99_08880"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX3635588.1"
                     /translation="MNQRDAATNGTATSSFDALSLRGWLAHLSATGRVATIAKPVALR
                     HELAAVAKRLDGKQAAVFLAPDGHDMPVVSGFMSRRAWIAEAMGVAEKDLLQRFRDAA
                     DHPLPCKEMPAGEAACQQVVHTSGIDLHALLPIPTHSEHDNGPYITAGLVIARNPRTG
                     VQNVSINRIQVHARDRMAILMLPRHLHAFQKTAEAAGDALDIAVAIGVDPLTMLASQA
                     ISPIDFDELEIAGALHGAPLPVVKCVTNEVRVPAFAEIVIEGRILPDVREAEGPFGEF
                     PKYYSAQEAREVIEVTALTHRQKPIYHTIVPAEMEHLLLGGIPREATLLSHLQRSHPG
                     VTDVHLSVGGVCRYHLWVQFAKKREGEAKNVILCAFGAHYDIKQVVVVDTDVDIHDPA
                     EIEWAIATRFQADRDLVLIEGAQGSPLDPSTTVGHPDDAPPHLQGISAKMGLDATRPV
                     AYSGHVFTRVRIPGYDAVDLDALVAPDNAGFDTYLSER"
     gene            4062..4685
                     /locus_tag="VHV99_08885"
     CDS             4062..4685
                     /locus_tag="VHV99_08885"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEX3635589.1"
                     /translation="MSVDQKPRLVVGISGASGAVIGVRLLAALRRIGTHETHLIVSAS
                     GAVTAAQELGITRHDLDAMADVVHNVRDIGASIASGSFVTSGMVVAPCSMKTLAAVAN
                     GFADNLLTRAADVMLKERRRLVLVARETPLNLAHLRNMTMATEMGAIVMPPVPAFYAH
                     PKTIDDVVDHTVGRILDLFSIEHREIARRWSGLADEFGTGRHEGADE"
     gene            4682..>6695
                     /locus_tag="VHV99_08890"
     CDS             4682..>6695
                     /locus_tag="VHV99_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006046922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX3635590.1"
                     /translation="MNQREQTGFAGIDARASEAIEPVQDAPLLKAKTTHVGKSLERFE
                     DPAILTGRGRYGDDLGIKPGTLHAAVLRSPHAHAELISINTTGALACEGVRAVLTRDD
                     LAAWSRPFVVGVKSKMEQWALATDRVRYVGEPVAVVMAESRALAEDALDLIKVEYKTL
                     YPVTSIEGALKSDAAVLHPNVETNVISDRRFRYGDPEAAFDRAPHRVKLVAHYPRNTC
                     APIECGVVIAEYLAGDEGYDVTSNFMGPFSLHAVMAMALNVPANRLRHKAPRDSGGSF
                     GVKQAVFPYVVLMCLASRKAGAPVKWVEDRLEHLSAATSATARLSTLEAAVESDGRIT
                     ALSYDQLEDCGGYLRAPEPATFYRMHGCLTGAYAIDNLLVRNRIVLTNKTPTGLVRGF
                     GCPQVYFALERLMQRIALELKLDVLDVYRRNFVAADAFPYRAAAGALLDSGNYQEALR
                     RALAEGGYDELHARREAARKEGRLYGIGFAAIVEPSVSNMGYITTVMPADARRKAGPK
                     SGAIASATVSVDLLGGVVVTIASTPAGQGHMTVCAQVVADVLGVSPQDVVVNVEFDTH
                     KDAWSVAAGNYSSRFAGAVAGTVHLAAVRVRDKIARIVSAQLGCAPEDVCFQGGRIYA
                     KRAEDRAQPFGRVAANAPHWAPALLPEGEEPGLRETVFWNPPNMAAP"
BASE COUNT         1307 a         2022 c         2125 g         1241 t
ORIGIN      
        1 agcgtcgcca cgcgttgcgc aaactcctcg tccttcgtcg tgaacacctc acgctcgcct
       61 gccggcgtgg tgacgtacat cctgtagatc acgtcctgcg tgatccatga caaatacccg
      121 acgaccgcaa tcattacgcc cagcagcaac gccggtcctg aaccaagaat tccgcccgtc
      181 accgccacga tcaacccgat cacgcccagc gcaaccggca agggcttctg tcgcggcacc
      241 atcaccaccg ttgcgccacg caaatcacgt aacggaaaca gttggcccgc cgcactcagg
      301 ccggcgcggg caaacgtaat acctcgttcg ttgaaaggga gatcctgttg catgggtcga
      361 agtcttgtcg gcggaaagaa aggcgcagat taacagacgc gcttgagccc ggggcatccc
      421 gccgatcgtc gcatgcgatc tcacacgcgc gcaaagccgg ccaggcactc ccgtcccgtc
      481 cgttacattt cgatactaat gcccgcccgt tttctgtcga tttcgacctt gctcacgcgt
      541 ctacagggtg aaaccgccct taaccggaga catcgatgaa attcctgtgc ctcattttct
      601 ttgacgaaca cctgctcgac gacctgtcga aacggcagta cgacgcactg gtcgacgaat
      661 ccctggcata cgacgaacgc cttcgagaga gcggtcagtt catcgcggcg caggcgctgc
      721 agtccgcgaa ggaagcggtc acgttgcggg tccgcggcga gcatgtctcg atcacggatg
      781 gtccgttcgc cgagacgaag gagcaggtgg gcggttttct tctcatcgag gcgcgcgaca
      841 tgaatgaagc catgcgcgtg gcttccggtt ttcccggcgc gcgcctgggc ggcgtggaag
      901 tgcgtccggt gaaggagttg cgggcgtcgt ggtcggacgc atgagttgca taacctgaaa
      961 ggagtagacg atgtcgacgc tattgaaagg cagtcacgcg ttcagcggtt ttgcggtgaa
     1021 ggatcttgcc gaagcaaagg cgttttacag caatacgctc ggtctcaagg tgacgagcga
     1081 gcccatgggt attctcaagc tgcatctggc gggcggcaac aacgtactgg tctatcccaa
     1141 gcctgatcat gtgccggcaa cgtacacggt gctgaacttc ccggtcgaca gtgtggaccg
     1201 ggcagtcgac acgctgagtg cgcgcggcgt gaatttcgaa cactacgaca tgccaggtct
     1261 caagaccgat gccaagggaa tttgccgcgg ctccgagggt ccggtgattg cctggttcaa
     1321 agaccctgcg ggtaatattt tctcggtgct cgaagagcaa tagaggtact aactacgaag
     1381 gtgctgacta ccttcacgct tgcatcgtcg actcgctgga caccagccgc aattcgtcgg
     1441 caatgatcgc aatcagcgct tccgctgccg cactcagcgg acgtcgcgga tgactgatcc
     1501 gcacaatatg gcgcacgatc cgcggcgcaa gaatgggata gacgcgcaac gtgcccaacc
     1561 gcaccgcgcg ctcgaccacg atgcgcggaa ggatcgtcgc gaagcgcgtg ctttccacca
     1621 gcttgacgat cgtgctcagc acgtcgatct caaagcgcgg cgcgagcagt acgtcttcgt
     1681 gctgggcggc ggtatcgagt acgccgcgca agccatggcg tttcgtgggc aacacgagtt
     1741 cgagttcagg aagctgcgca agctcgatga catgcggcaa gtcaggacca tgcacggcgc
     1801 tggtgacaag caccatctct tcatcgagca agggctggga gtccagcgac aggcgcgagc
     1861 gaggtttgtt aatgagcgca gcgtcgagtt gccctcccga tacccagtcg ataaaggtcg
     1921 cgctgtaacc gtctgacacg gtgacttcca catgcggata acgagcgtga aaccgcgaca
     1981 gtgaatccgc cagcacgctc tccgtcaccg acgcaatcaa gccgatcgat acatgccccg
     2041 tcaccacctc gtcacgctgc accagctgct ggcgcgcatg tgcgagatcc cgcatgatag
     2101 gcaggaagag gcggtacatc agccgtcccg ctgcggtcgg ggccataccg tgcgcgccgc
     2161 gttcaaagag ctgctgatgc agttcgtcct ccagcttggc gatctgcatg ctcaaggcag
     2221 gctgcacgat gttcagccgc ttggctgccc gggtgacgga gccgtcctcg aacagggcga
     2281 tgaaatactg aatctgcttc aggtccatgt aggtcggctc agtaagaatt ggtccggcgg
     2341 tcaggctatc agtcgggcta tcagtcaggc tatcaataag tctaatgaag cagcggacat
     2401 ttatcagacg ccagcccgga gggtactgcg cccaagcccc atgctattga tattccacta
     2461 acaaaatact ttcattggga caaatacgga tatcacggag actgatcgaa agcatcaaaa
     2521 aacagtatta gaagttatag cgcgttgtcg ttacgcttca tttcaggaat tgatgaagac
     2581 tcgatggaga cgcagatgaa ccaacgtgac gccgcgacga acggcaccgc aacaagctcc
     2641 ttcgacgcgt tgtcgctacg tggctggctt gcgcacctct ccgccaccgg acgtgttgcg
     2701 accatcgcca aaccggtcgc gctgagacat gagctcgcgg ccgtggcgaa gcggctcgac
     2761 ggcaagcaag cggcagtatt tctcgccccc gatggccacg atatgccggt ggtcagcggc
     2821 ttcatgtcca ggcgtgcgtg gattgccgag gccatgggcg tggcggagaa agacctgttg
     2881 cagcgttttc gcgatgcagc agatcatccg ctgccatgca aggaaatgcc cgcgggggaa
     2941 gccgcttgcc agcaggtcgt gcataccagc ggtatcgacc ttcacgcgtt gctgccgatc
     3001 cccacccata gcgagcatga caacggtccg tatatcaccg cgggtctcgt gattgcgcgt
     3061 aatccgcgca ctggcgtgca gaacgtttcg atcaaccgta tccaggttca cgctcgcgat
     3121 cgcatggcga tcctgatgct gccgcgccat cttcatgcgt tccagaaaac cgcggaagcg
     3181 gcgggcgatg cgctcgatat cgcggtggcc atcggcgtcg atcccctgac catgctcgca
     3241 tcccaagcca tttctccgat cgatttcgat gagctggaga ttgctggcgc actgcacggc
     3301 gcgcccttgc ctgtcgtcaa atgcgtgacg aacgaggtgc gcgtgccggc gttcgcggag
     3361 atcgtgatcg agggccgcat cctgccggac gtgcgcgaag cggaaggccc gttcggcgaa
     3421 tttccgaagt actacagcgc acaggaagca cgtgaagtaa tcgaggtgac tgcgctcacg
     3481 catcggcaga agccgattta tcacaccatc gtgcccgccg aaatggagca cttgctgctg
     3541 ggcggtattc cgcgcgaggc gacgctttta tcgcaccttc aacgcagtca tcctggcgtg
     3601 accgacgtgc atctttcagt gggcggcgta tgccgttatc acctgtgggt gcagttcgcg
     3661 aagaagcgcg agggcgaggc gaagaacgtg atcctgtgtg cattcggcgc gcattacgac
     3721 atcaagcagg tcgtggtggt ggataccgac gtggacattc atgatcccgc cgaaattgag
     3781 tgggcgatcg ccacgcgttt ccaggcggac cgcgatcttg tgctgattga aggcgcgcaa
     3841 ggctcgccgc tggacccgtc gacaaccgtc ggtcatcctg acgatgcacc cccgcatctg
     3901 cagggcatca gcgcgaagat gggtctcgac gcgacgcggc cggtagccta ttcgggccat
     3961 gtgttcaccc gggtgcggat tcccggctac gacgcggtcg atctcgacgc gctggtggcg
     4021 cccgataacg ccggcttcga cacctatctg agcgagcgtt gatgagcgtc gaccaaaagc
     4081 cacgcctggt cgtcggcatt tcgggagcga gcggtgcggt cattggcgtg cggctgcttg
     4141 cagcactgcg ccggatcggt acgcacgaga cgcatctgat tgtgtctgcg tcgggagcgg
     4201 tgacagcagc gcaagaactc ggcatcacgc gccatgatct cgacgcgatg gccgacgttg
     4261 tgcataacgt ccgcgatatc ggcgcgtcga ttgcgagcgg ttcgttcgtg acgagcggca
     4321 tggtagttgc tccctgttca atgaaaacgc tggctgcggt ggcgaatggt tttgcggaca
     4381 acctcctcac gcgcgcagcc gacgtgatgc tcaaagagcg ccgccgcctg gtgctggtgg
     4441 cgcgcgaaac gccgctcaat ctggcgcact tgcgcaacat gaccatggcg actgaaatgg
     4501 gtgcgatcgt gatgccgccg gtgccggcgt tctatgcgca tccgaagacc atcgacgatg
     4561 tcgttgacca taccgtcgga cggattctcg atctgttcag catcgagcac cgtgaaatag
     4621 cccggcgatg gagcggtctc gccgatgagt tcggcacagg acgccacgaa ggagcagacg
     4681 aatgaaccag cgcgaacaga ccggctttgc cggtatcgac gcgcgcgcgt cagaggcgat
     4741 cgagccagtg caagatgcgc ccttgctgaa ggcgaaaacc actcacgtcg gcaagtcgct
     4801 cgaacgtttc gaagaccccg ccatcctgac tggccgcggc cgttatggcg acgacctggg
     4861 tatcaagccg ggcacgctgc acgcagccgt gctgcgctcg ccgcacgccc atgctgaact
     4921 gatttccatc aataccaccg gtgcactcgc atgcgaagga gtgcgagcgg ttctgacgcg
     4981 tgacgacctc gctgcgtggt cacgcccgtt cgtggtcggg gtgaagtcga agatggaaca
     5041 gtgggcgctc gcgaccgatc gtgtgcgcta cgtgggcgaa cccgttgcgg tcgtgatggc
     5101 tgaatcgcgt gcgctggccg aggacgcgct cgacctgatc aaggtggaat acaagacgct
     5161 ctacccggtg acgtcgatag aaggcgcgct caaaagcgac gcggcggtgc tgcatccgaa
     5221 cgtcgagacc aacgtgatca gcgaccgtcg cttccgctat ggcgatcccg aagctgcgtt
     5281 cgatagagcg ccacatcgcg tgaaactggt cgcgcattat ccgcgcaata cctgcgcgcc
     5341 gatcgaatgc ggcgtggtca tcgccgaata tctcgcgggc gacgaaggct acgacgttac
     5401 gtcgaatttc atggggccgt tctcgctgca cgcggtgatg gcgatggcgc tgaacgtgcc
     5461 cgccaaccgt ctgcgtcaca aggcgccgcg cgattcgggc ggcagcttcg gcgtgaagca
     5521 ggcggtgttt ccgtatgtcg tgctgatgtg ccttgcctcg cgcaaggctg gcgcgccggt
     5581 gaagtgggtc gaggaccggc tcgaacatct gagcgcggcg acgtcggcga cggcccgcct
     5641 gtcgacgctc gaggcggctg tcgaaagcga cggccgtatt accgcgctct cctacgatca
     5701 actcgaagat tgcggcggct atctgcgcgc gccggaaccg gcgacgttct accggatgca
     5761 tggctgcctg acaggcgcgt atgccatcga caacctgctg gtgcgcaacc ggatcgtgct
     5821 gaccaataag acgcccacgg gtctcgtgcg gggcttcggc tgcccgcagg tttacttcgc
     5881 gctcgaacga ctgatgcaac gcatcgcgct cgagttgaag ctagatgtgc tcgacgtcta
     5941 tcgtcgcaat ttcgtcgccg ccgatgcgtt cccatatcgc gccgcagcgg gcgccctgct
     6001 cgactcgggc aactatcagg aggcgctgcg ccgtgcgctg gccgaaggcg gctatgacga
     6061 actgcacgcg cgccgcgagg cggcgcgcaa ggaagggcgg ctgtacggca tcggcttcgc
     6121 ggcgatcgtg gagccctccg tgtcgaacat gggctatatc acgacggtga tgccggccga
     6181 cgcgcgcagg aaggccggcc cgaagagcgg cgccatcgcg agcgcgacgg tcagcgtcga
     6241 tctgctaggc ggcgtggtgg tgacgatcgc gtcgacgccc gcaggccagg ggcatatgac
     6301 cgtgtgcgcg caggtggtcg cagatgtgct gggcgtctcg ccgcaagacg tggtcgtcaa
     6361 tgtcgagttc gatacgcata aggacgcgtg gtcggtggcg gcgggcaatt attcgagccg
     6421 ctttgccggc gcggtggcag gcaccgtgca tctggcggcc gtgcgcgtac gcgacaagat
     6481 cgcgcgcatc gtgtcggcgc agttgggttg cgcgccggaa gacgtctgtt tccaaggcgg
     6541 ccgcatttat gccaagagag cagaggaccg cgcgcagccg ttcggccgcg ttgccgccaa
     6601 tgcgccgcat tgggcgcccg cgctcttgcc cgaaggcgag gagcccggcc tgcgcgagac
     6661 cgtgttctgg aatccgccga acatggcggc gccgg
//
LOCUS       DATASH010000316         3519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_439672,
            whole genome shotgun sequence.
ACCESSION   DATASH010000316 DATASH010000000
VERSION     DATASH010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439672"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VHV99_08895"
     CDS             complement(<1..193)
                     /locus_tag="VHV99_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635591.1"
                     /translation="MKWLLLFEVFLPARRLFVSVLGGLAFPCSGFRAFPCFVSGLLAL
                     PLCGAALTFFAAAKKDKQRK"
     gene            complement(171..1406)
                     /locus_tag="VHV99_08900"
     CDS             complement(171..1406)
                     /locus_tag="VHV99_08900"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018432563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HEX3635592.1"
                     /translation="MNIYAVGGAIRDELLGVPVQDRDYVVVGATPEQMVAQGYRPVGK
                     DFPVFLHPQTHEEYALARTERKTAAGYHGFQFFYAPDVTLEEDLARRDLTINAMAREM
                     RPDGELTGPVIDPFNGQGDLQARLFRHVSDAFLEDPVRILRIARFAARFVDFTVAPET
                     MALMRKMVADGEVDALVAERVWQEVSRGLMEKKPSRMFEVLRECGALARILPEIDALF
                     GVPQRADYHPEVDTGVHVMMVIDHAAQQGYALPVRFAALTHDLGKATTPDDILPRHIG
                     HEGRSVDLLKPLCERLRVPNDCRDLALLVAREHGNIHRVMEMGAAALVRLLERSDAIR
                     KPARFAEALQACEADARGRLGFEMREYPQAERLRVALVAARGVDAGAVAKRLVDSPAG
                     IKDAVHQERVRAVEMALVV"
     gene            complement(1403..2056)
                     /locus_tag="VHV99_08905"
     CDS             complement(1403..2056)
                     /locus_tag="VHV99_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX3635593.1"
                     /translation="MKLLIGDKNYSSWSMRPWLLFKHFGIPFEEVLIHLREPGTKAAI
                     LSHAPASAGKMPTLVADDGLVVWDSLAIAETLAERFPQHALWPRDPVARGFARSISAE
                     MHSGFGALRSNMWMNIRASFPGKNATPEALADIARIDAIWRKCLDTYGGPFLFGAFGI
                     ADAMYAPVVMRFNTWQPALSEAAAAYVRRVTAEPAVKAWIDDARRETHTLPDQDVCP"
     gene            complement(2087..3040)
                     /locus_tag="VHV99_08910"
     CDS             complement(2087..3040)
                     /locus_tag="VHV99_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HEX3635594.1"
                     /translation="MRHQAIAVIGGSGFIGSHLVNALVEMGKDVRIATRRRYNARHLT
                     LLPIDVIEADVFDPVQLARFVEGADCVINLVATLHGKRGKPYGPEFARTHVELPTRIV
                     AACEGKGVHRLIHISALGADSNGPSMYSRSKGDGEKAVHAANLAWTIFRPSVVFGPED
                     QLLNKFAFLQRVFPAIPLAMPDAKFQPVYVGDVAKAICNVLDLDAASGHTYELGGPTV
                     YTLEDLVTYCGAVIGRHARIVRLPEALARLQALTFEMAPGEPVISRDNLDSMKVDNVL
                     SGPLAPELGIEPASIETIAPVYLTGASTRSRFDTFRASAGR"
     gene            complement(3124..>3519)
                     /locus_tag="VHV99_08915"
     CDS             complement(3124..>3519)
                     /locus_tag="VHV99_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX3635595.1"
                     /translation="SEVGASGLMQLMPGTAQLVAKKIGLGPISREQMNDINTNILLGT
                     NYLSMIYNQFDGSAVLATAGYNAGPGRPRNWRQQLQHPVEGAIFAESIPFQETRDYVK
                     NVLSNTVYYAALFEGRPQSLKTRLGYIAP"
BASE COUNT          742 a         1181 c          995 g          601 t
ORIGIN      
        1 gctttctttg cttatctttc tttgcggcgg caaagaaagt aagtgccgcc ccgcacaggg
       61 gcaacgctaa tagaccacta acaaaacaag gaaaggcacg aaagccagaa caaggaaagg
      121 ccaacccccc aagaacactg acaaacaagc gccgcgcagg caaaaaaacc tcaaacaaca
      181 agagccattt caacagcgcg aacgcgctcc tgatgcactg catccttaat ccccgcaggc
      241 gagtcaacga gccgcttagc cacggcacca gcatccaccc cccgcgcagc aacaagcgca
      301 acccgcagcc gctcagcctg cggatactcc cgcatctcaa acccaagccg cccacgcgca
      361 tccgcctcgc aagcctgcaa ggcttcagcg aaccgcgcag gtttccgaat cgcatcgctg
      421 cgctcgagca gcctcaccaa cgcggcagcc cccatctcca tcacgcgatg aatgttccca
      481 tgctcccgcg ccaccagcaa cgccagatcg cgacaatcat tcggtacccg caaccgctca
      541 cataagggct tcagcaaatc cacgctgcgc ccctcatgcc cgatatgccg cggcaagata
      601 tcgtccgggg tcgtcgcttt gcccaagtca tgcgtcaacg ccgcaaaccg caccggcaac
      661 gcgtacccct gctgcgcagc atggtcgatc accatcatca catgcacgcc cgtatctacc
      721 tcggggtgat agtcagcccg ctgcggcacg ccgaacaatg cgtcgatctc cggcagaatc
      781 cgcgccaacg caccgcattc ccgcagcacc tcgaacatcc gcgacggctt cttctccatc
      841 aacccgcgcg atacttcctg ccacacgcgc tcggccacca gcgcatccac ctcaccgtcg
      901 gccaccatct tgcgcatcag cgccatcgtc tccggcgcaa cggtgaaatc gacgaagcgc
      961 gccgcaaacc gtgcaatccg caaaatccgc acggggtcct caagaaacgc atcgctgacg
     1021 tgacgaaaca gccgcgcctg caaatcgccc tggccgttaa acgggtcgat caccgggccg
     1081 gtcaactcgc catccgggcg catctcacgc gccatcgcgt tgatcgtcag gtcgcggcga
     1141 gcgagatcct cttcgagcgt gacgtcgggt gcatagaaga actggaaacc gtgatagccg
     1201 gccgccgtct tgcgctccgt gcgcgcaagt gcgtactcct cgtgcgtctg cggatgcagg
     1261 aacacgggga agtccttgcc caccggccga tagccctgcg ccaccatctg ctcgggcgtc
     1321 gcgccaacca ccacgtagtc acggtcctgt accggcacgc ccaatagttc gtcgcgaatc
     1381 gctccgccta ccgcatagat attcatgggc atacgtcttg atcaggaagt gtgtgcgttt
     1441 cacgccgcgc gtcgtcgatc catgccttca cggcgggctc ggccgtcacg cgacgaacat
     1501 aagccgcggc ggcctcggac aacgccggct gccacgtgtt gaagcgcatc actaccggcg
     1561 cgtacatcgc gtcggcaatg ccgaacgcgc cgaacaggaa ggggccaccg tatgtatcca
     1621 ggcacttgcg ccagatcgcg tcgatgcgcg caatgtcggc caatgcctcc ggcgtcgcgt
     1681 tcttgcccgg aaacgacgcg cgaatgttca tccacatgtt cgaacgtaac gcaccaaaac
     1741 ccgaatgcat ttcggcgctg atgctgcgcg cgaacccgcg cgccacagga tcacgcggcc
     1801 acagcgcatg ctgcgggaag cgctcagcca gcgtctcggc gatcgcgagc gaatcccaca
     1861 cgacgaggcc gtcgtcggca accagagtcg gcattttgcc cgcgctcgcc ggcgcgtgag
     1921 acagaatcgc ggccttcgtg ccgggctcac gcaagtggat cagcacttcc tcaaagggaa
     1981 tgccgaaatg tttgaacaac agccacggac gcatcgacca tgacgaatag ttcttgtcac
     2041 caataagcag cttcatgctt cgaataggaa gagggataaa aaaggttcag cgccccgcgc
     2101 tggcgcgaaa cgtgtcgaag cgcgagcgcg tcgacgcgcc agtcagatac accggcgcga
     2161 tcgtttcgat gctggcgggt tcgatgccga gttcgggcgc aagcggcccg ctcagcacgt
     2221 tgtcgacttt catcgaatcg agattgtcgc gcgagatcac cggttcgccc ggcgccattt
     2281 cgaacgtcag cgcttgcagg cgcgccagtg cttccggcag gcgaacgatc cgcgcatgcc
     2341 ggccaatcac agcgccacaa tacgtgacga ggtcttcgag ggtatagacg gtcgggccgc
     2401 ccagttcgta ggtgtggccg ctcgctgcat cgagatcgag cacgttgcag atcgccttcg
     2461 ctacgtcgcc cacgtacacc ggttggaatt tcgcgtccgg catggcgagc gggatcgccg
     2521 ggaacacccg ctgcaggaat gcgaacttgt tgagaagctg atcttcggga ccgaacacca
     2581 cggatggccg gaaaatcgtc cacgccagat tcgcagcgtg cacggctttt tcaccgtcac
     2641 ccttcgagcg cgaatacatg ctcgggccgt tggaatcggc accgagcgca ctgatatgaa
     2701 tcaaccgatg cacgcccttg ccttcgcaag ccgcaacgat tctggtcggc aattcgacgt
     2761 gcgtgcgcgc gaattccggg ccgtatggct tgccgcgctt gccatgcaac gtggcaacga
     2821 ggttgatcac acaatcggcg ccttcgacga aacgcgcgag ctgcaccgga tcgaacacgt
     2881 ccgcttcgat cacgtcgatg ggtaacaggg tgagatggcg ggcgttgtaa cgccgccggg
     2941 tggcgatgcg cacgtctttg cccatttcaa caagggcgtt gacgagatgg ctgccgataa
     3001 acccggaccc gccgatgacc gcaatggctt gatgtcgcat ttttcgactc cctggtggaa
     3061 gccgcggcaa cagctggcgt ggcgcaccgc cgcgtcagaa gtgacgcggc ggcggcacgg
     3121 cgcttacggc gcgatataac ccagacgcgt cttcagcgat tgcggacggc cttcgaacaa
     3181 tgccgcgtag tagaccgtgt tggacaacac attcttaacg tagtcgcgcg tttcctggaa
     3241 cggaatagac tcggcgaaga tcgcgccttc taccggatgc tgcaactgtt gccgccagtt
     3301 gcgcgggcgg ccgggaccgg cgttatagcc ggcggtggcc agcacggctg acccgtcgaa
     3361 ctgattgtag atcatcgaca ggtagttcgt gcccagcagg atgttggtgt tgatgtcgtt
     3421 catctgctcg cgcgaaatcg ggccgaggcc gatctttttc gccaccagtt gcgcggtgcc
     3481 cggcatcaac tgcatcaaac cgctcgcccc gacttccga
//
LOCUS       DATASH010000317         5244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4397566,
            whole genome shotgun sequence.
ACCESSION   DATASH010000317 DATASH010000000
VERSION     DATASH010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5244
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4397566"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(238..555)
                     /gene="cyaY"
                     /locus_tag="VHV99_08920"
     CDS             complement(238..555)
                     /gene="cyaY"
                     /locus_tag="VHV99_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HEX3635596.1"
                     /translation="MSDSDYLTRAEAALAAIERALDDTEADIELERSGNVLTLEFENR
                     SKIIVNLQPPMSEIWIAAKAGGFHFRFVDGEWRDTRSGTEFFAALSEYATQQAGEPVH
                     FEA"
     gene            591..944
                     /locus_tag="VHV99_08925"
     CDS             591..944
                     /locus_tag="VHV99_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEX3635597.1"
                     /translation="MRVVSRMRAALPGRAIVAGLAILAGCALAGCGQRGSLYLPTVPP
                     LPAKPIDRTQPPSSPSSEEVKPGAENASPTGEVPDTSGAPLSLSPESELAAPPASAAS
                     AATPAQPASAVTPVQ"
     gene            954..2216
                     /gene="lysA"
                     /locus_tag="VHV99_08930"
     CDS             954..2216
                     /gene="lysA"
                     /locus_tag="VHV99_08930"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX3635598.1"
                     /translation="MTRSAFDYVDGVLHAEGVSAVSLAEQFGTPLYVYSRAALTEAWN
                     AYAGACAGRRATVHVAVKANSNLAVLNVFARLGAGFDIVSGGELARVLAAGGKAENTV
                     FSGIGKHADEMRQALAAGVKCFNVESIPELDRLNTVAAEMGKKAPVSLRVNPDVDAKT
                     HPYISTGLKSNKFGVAFEDARATYQAAAAMTHLEVVGIDCHIGSQITEVAPYLDAVDK
                     VLELVEQIEQDGVKIRHIDVGGGLGITYDDETPPEIGDFVRTVLDRIEARGHGHREVY
                     FEPGRSLVGNAGVLLTRVEFLKPGAEKNFAIVDAAMTDLARPAMYEAYHAIEAVVKRD
                     VPAHVYDVVGPVCESGDWLGRERLLAVEPGDLLAIRSAGAYGFVMSSNYNTRPRAAEV
                     MVDGTQGHVVRAREEVKQLFAGETILPA"
     gene            complement(2309..3004)
                     /gene="msrQ"
                     /locus_tag="VHV99_08935"
     CDS             complement(2309..3004)
                     /gene="msrQ"
                     /locus_tag="VHV99_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HEX3635599.1"
                     /translation="MASSDSQPVIETARTTPGPKASATARRPASGARWIVPARIVVFI
                     AAWYPLARIVLFGMTDRLGANPIEFITRSTGLWTLVFLCITLAVTPLRRLTGIAAFVR
                     FRRMLGLYAFFYATLHFTTYLWFDKWFDVAAILKDIGKRPFITVGFAAFVLLIVLAVT
                     SPRAMVRKLGRRWQTLHRAIYAIGGLAILHFWWMKAGKHDLILPKIYGAIMVALLGWR
                     LIVWLRERRLKAR"
     gene            complement(3019..4014)
                     /gene="msrP"
                     /locus_tag="VHV99_08940"
     CDS             complement(3019..4014)
                     /gene="msrP"
                     /locus_tag="VHV99_08940"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009913838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HEX3635600.1"
                     /translation="MWIKRSDRIQLIGDDIARSEITPQRVFENRRRVLQAAGALALGS
                     LVGVNGEALAAYSSPDPKAQKLTAKTNAKFVALDKITPYKDITTYNNYYEFGTDKADP
                     AHNAGTLRPHPWKVSVEGEIKNPKVYDIDELLKLAPLEERVYRLRCVEGWSMVIPWIG
                     VPLSELIKRVQPTGNAKYVQFITLADPSQMPGLSEPILEWPYREGLRMDEAMNPLTLL
                     TMGLYGQVLPNQNGAPIRVVVPWKYGFKSAKSLVKIRFLDKQPPTSWNTYASNEYGFY
                     SNVNPNVDHPRWSQATERRIGEDGFFTPKRKTLMFNGYGDQVASLYQGMDLKKNF"
     gene            complement(4213..>5244)
                     /gene="ccsB"
                     /locus_tag="VHV99_08945"
     CDS             complement(4213..>5244)
                     /gene="ccsB"
                     /locus_tag="VHV99_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011655767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEX3635601.1"
                     /translation="YHPFMNYYDKLVLCCAVPVFVVLGWRWKPVRPLMVGIAALSLLA
                     IQIYQGDLTRADNTFFLKYFLSSQSAILWMSALFVFATVFYWIGLLSRSPTGAAIGSK
                     MTWAAVVMGFVGLMVRWYESYLIGADVGHIPISNLYEVFVLFSLITALFYLYYEQHYN
                     TRALGAFVLLVISAAVGFLMWYSVARDAQQIQPLVPALQSWWMKIHVPANFIGYGSFA
                     LSAMVGVAYLAKEHGVLADRLPALDVLDDLMYKSIAVGFAFFTIATILGALWAAEAWG
                     GYWSWDPKETWALIVWLNYAAWLHMRLMKGLRGAVAAWWALTGLLVTTFAFLGVNMFL
                     SGLHSYGKL"
BASE COUNT         1071 a         1650 c         1561 g          962 t
ORIGIN      
        1 caggcaacgg tttcgtcgcg acggtcggcg tcagtcaggc ggccttggat gccgacgcga
       61 gcggtgcgtc cgcggcggcg cccgagcaag tcggcgagca ggaaaagcag gacatcatga
      121 atctgttccg cggacactga aagagcctcc gcgagggagg ctccaaagtc cattgaaaaa
      181 aaaccgccgc gctggatgcg cggcggtttc atttggacgg tggcttgggt ggtggcttta
      241 cgcttcgaag tgaaccggct cgccagcctg ttgcgtcgcg tactccgaca gcgcggcaaa
      301 aaactccgtg ccgctgcggg tatcgcgcca ctcaccgtcg acgaagcgga agtgaaaacc
      361 accagctttc gcggcgatcc agatttcgct catcggcggc tgcagattga cgatgatctt
      421 cgagcggttc tcgaattcga gggtcaggac attgccgctg cgctcgagtt cgatgtcggc
      481 ttccgtgtcg tcgagcgcgc gttcgatggc ggctagcgcg gcttccgcgc gggtcaggta
      541 atcactatcg gacatgctaa actccatcga ttatttattc agggacagtc atgcgagtcg
      601 tatctcggat gcgcgcggca ctgcccggcc gcgcgattgt agcgggttta gccattctcg
      661 caggttgtgc acttgcgggc tgcgggcaac gcggttcgct ctatttgccc accgtgccgc
      721 cgctgccggc caagccgatc gatcgcacgc aaccgccgtc gtcaccgtca tcagaagaag
      781 tgaaaccggg cgcggaaaac gcgtcgccga ctggtgaggt acccgacact tctggcgcgc
      841 cgctgtcgtt gtcgccggaa agcgaactcg ccgcaccgcc cgcgtctgcc gcatccgccg
      901 caacgcctgc gcaacctgcc tccgccgtca ctcctgttca ataagacttt cgcatgactc
      961 gatccgcatt tgactacgtc gacggcgtgc tgcacgccga aggcgtgtcc gccgtctcac
     1021 tcgccgaaca gttcggcacg ccgctgtacg tctattcgcg cgccgcgctc accgaagcat
     1081 ggaacgccta cgcgggcgct tgcgccggcc gccgcgcaac cgtgcatgtc gccgtcaagg
     1141 ccaatagcaa tctcgcggtg ctgaatgtgt tcgcgcgcct cggtgccggc ttcgacatcg
     1201 tgtcgggcgg cgaactggcg cgtgtgctgg ccgccggcgg caaagcggaa aacaccgtat
     1261 tttccggcat cggcaagcac gcggacgaga tgcgtcaggc actcgcggcc ggcgtcaaat
     1321 gcttcaacgt cgaatcgatt cccgagctcg accgcctgaa tacggtggcc gcggaaatgg
     1381 gcaagaaggc gcccgtctcg ctgcgcgtga atccggatgt cgacgcgaaa acgcatcctt
     1441 atatttccac tggcctgaag tcgaacaaat tcggcgtcgc gttcgaagac gcgcgggcaa
     1501 cctatcaagc cgccgcggca atgacgcatc tcgaggtggt cggcatcgac tgccatatcg
     1561 gctcgcagat cactgaagtc gcgccgtatc tggacgcggt cgacaaggtg ttggaactgg
     1621 tcgaacagat cgaacaggac ggcgtgaaga ttcgccacat cgacgtaggc ggcggtctcg
     1681 gcatcacgta tgacgacgaa accccgccgg aaatcggcga cttcgtacgc accgtgctgg
     1741 atcgcatcga agcgcgcggc cacgggcatc gcgaagtgta tttcgaaccg ggccgttcac
     1801 tggtcggcaa tgcgggtgtg ctgctgacgc gcgtcgagtt tctgaagcct ggcgccgaga
     1861 aaaacttcgc tatcgtcgat gcagcgatga ccgatctcgc acgccccgcg atgtacgagg
     1921 cctatcacgc aatcgaggcc gtcgttaagc gcgacgtgcc cgctcacgtg tacgacgtgg
     1981 tcggcccggt ttgcgaaagc ggcgactggc tgggtcgtga gcgtctgctg gcggtcgaac
     2041 cgggcgatct gctggcgatt cgctcggcag gcgcgtatgg cttcgtgatg agctcgaact
     2101 acaacacccg tccgcgtgcg gccgaggtga tggttgatgg tacacaaggg catgtcgttc
     2161 gcgctcgtga agaggtcaag cagctgtttg cgggcgagac gattttgccg gcgtaaacgt
     2221 cagagctttg aactgttcca tgaaaaaggc gatgccgctg cggcatcgcc ttttttcatt
     2281 gacgcgcggt agataagggc cgcacaggtc agcgcgcctt taagcgccgc tcgcgcagcc
     2341 atacgatcaa acgccacccc agcaacgcga ccatgatcgc gccgtagatc ttcggcaaaa
     2401 tcaggtcgtg cttgcccgcc ttcatccacc agaagtgcag aatggcgagc ccgccgatcg
     2461 catagatcgc gcgatgcagc gtctgccagc ggcgcccaag tttgcgcacc atcgcgcgcg
     2521 gcgacgtcac ggccaacacg atcagcaaca cgaacgcagc gaagcccacg gtgatgaacg
     2581 gccgcttgcc gatgtccttg agaatcgcgg caacatcgaa ccacttgtcg aaccacaggt
     2641 aagtggtgaa atgcagcgtc gcgtagaaga atgcgtaaag acccagcatg cgacgaaacc
     2701 tgacgaatgc agcgatgccg gtcagccggc gcaagggcgt cacggccaac gtgatgcaaa
     2761 gaaagacgag cgtccacaga cccgtcgagc gcgtgatgaa ctcgatcgga ttcgcgccca
     2821 accgatcggt catgccgaac agcacgatgc gcgcgagcgg ataccacgcc gcgatgaaca
     2881 cgacgatcct ggccggcacg atccagcgcg cgccgcttgc cgggcgcctt gccgtcgctg
     2941 acgctttggg gcctggcgtc gtgcgcgcgg tttcaataac aggttgcgaa tcggaggaag
     3001 ccatgattac gtcgtcattc aaaaattctt cttcaggtcc atgccctgat agagcgacgc
     3061 gacctgatcg ccgtagccgt tgaacatcaa ggtcttgcgc ttgggcgtga agaacccgtc
     3121 ttcgccgata cgccgctccg tggcctggct ccagcgcggg tgatcgacgt tcggattcac
     3181 gttcgagtaa aagccgtatt cgtttgacgc atacgtgttc cagctggtcg gcggctgctt
     3241 gtcgagaaag cggatcttca ccagcgactt cgcgctcttg aagccgtatt tccacggcac
     3301 caccacgcgg atcggcgcac cgttctgatt cggcagcacc tggccgtaga gccccatggt
     3361 cagcagcgtc aacggattca tcgcttcgtc catccgcaga ccttccctgt acggccactc
     3421 gagtatcggt tccgacagac caggcatttg cgacggatcg gccagcgtga tgaattgcac
     3481 gtacttggcg ttgcccgtgg gctgcacgcg cttgatcaat tccgagagcg gcacaccgat
     3541 ccacggaatc accatcgacc aaccttcgac acaacgcaac cggtacacgc gctcctcgag
     3601 cggcgcgagc ttcagcaatt cgtcgatgtc atacaccttc ggattcttga tctcaccctc
     3661 gacgctcact ttccacgggt gcggccgcaa ggtaccggcg ttatgcgccg gatcggcctt
     3721 gtcggtgccg aactcgtagt agttgttgta cgtggtgatg tccttgtacg gcgtgatctt
     3781 gtcgagcgcg acgaacttcg cgttggtctt cgccgtgagc ttctgcgcct tcggatccgg
     3841 cgatgaatac gccgccagcg cttcgccatt caccccgacc agactgccga gcgctaacgc
     3901 gcccgccgcc tgcaacacgc gccgccgatt ctcgaagacg cgctgcggcg tgatttcgct
     3961 gcgcgcaatg tcatcgccga tgagttgaat acggtcgctt cgcttgatcc acatcgtgct
     4021 gctccttgcc ggcccatcgg gttgtctatc ttacggggcc atattgatga tcggctcgtt
     4081 tagccactcg tccagccatc cataggtact gttctaacga acacactgga tgcagcaaat
     4141 attccgccag acataaaaaa accgccggtg cgagccacgc ggcggttctt ggtcggcttg
     4201 gaacgacgga ccttacagct tgccgtagct atgcagaccc gacaggaaca tgttgacgcc
     4261 gaggaacgca aaggtcgtca ccaacaggcc ggtcaacgcc caccatgccg ccactgcgcc
     4321 gcgcaggccc ttcatcaagc gcatatgaag ccacgccgcg tagttcagcc agacgatcag
     4381 cgcccacgtt tccttcgggt cccaactcca gtaaccgccc catgcttcag cggcccacag
     4441 cgcgccgagg atcgtcgcga tcgtgaagaa cgcaaagccg acggcgatcg acttgtacat
     4501 caggtcgtcg agcacatcga gcgccggcag gcggtctgcc agcacaccgt gctccttcgc
     4561 cagatacgcg acgccgacca tcgccgacag cgcgaagctg ccatagccga tgaagttagc
     4621 cggcacgtgg atcttcatcc accagctttg cagcgcgggc acgagcggct gaatctgctg
     4681 tgcgtcacgg gcaaccgaat accacatcag gaaaccgaca gccgcgctga tcaccagcag
     4741 cacaaacgcg ccgagcgcgc gggtgttgta gtgctgctcg tagtacagat agaacaatgc
     4801 ggtaatcagg ctgaacagca cgaacacttc atacaggttc gagattggaa tatggccgac
     4861 gtccgctccg atcaggtacg actcgtacca gcgcaccatc agaccgacga agcccatcac
     4921 aaccgcagcc cacgtcattt tcgaaccgat cgcggcaccg gttggtgaac gcgacagcag
     4981 gccgatccag tagaagacgg tggcgaacac aaagagcgca ctcatccaca gaatcgcgga
     5041 ctggctcgac aggaaatatt taaggaaaaa ggtgttatcc gcgcgcgtca gatcgccctg
     5101 atagatctgg atcgccagca gcgacagcgc ggcgatgccc accatcagcg ggcgcaccgg
     5161 cttccagcgc cagcccagca ccacgaacac cggcacggcg cagcacagca ccagcttgtc
     5221 atagtaattc atgaacgggt ggta
//
LOCUS       DATASH010000318        11164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6967999,
            whole genome shotgun sequence.
ACCESSION   DATASH010000318 DATASH010000000
VERSION     DATASH010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 11164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11164
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6967999"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..665
                     /locus_tag="VHV99_08950"
     CDS             33..665
                     /locus_tag="VHV99_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX3635602.1"
                     /translation="MNQPKIKRDPEGTRRRILLAAAEEFANGGLFGARVDQIARRAET
                     NERMLYYYFGSKEQLFTAVLEHAFSALNEAERTLELNGVAPVEAVTRLAHFVWDYYRD
                     HPELLRLINNENLHEARYMQKSTRIREMISPIVATLGGILERGQRAGLFRTNVDPLRF
                     YVTLSGMGYYIVSNRFTLEATLGRDFSSASERSEVIQMNTELLLAYLLRK"
     gene            complement(772..2049)
                     /gene="phaZ"
                     /locus_tag="VHV99_08955"
     CDS             complement(772..2049)
                     /gene="phaZ"
                     /locus_tag="VHV99_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HEX3635603.1"
                     /translation="MLYQLHEFQRAMLSPLTAWAQAASKSFANPASPLAYVPGATRLS
                     AGYELLYRLGKDYEKPEFNLHQIVKDGHNIPIIEQTIIEKPFCRLMHFKRFADDSDAV
                     IQLKDEPIVLVCAPLSGHHATLLRDTVRTLLQDHKVYLTDWIDARMVPLEEGEFNLDD
                     YVAYIQEFIRHIGAKNLHVISVCQPTVPVLAAISLMASRGEDTPRTMTMMGGPIDARK
                     SPTSVNSLATQHSYEWFENNVIFTVPPNYPGVGRKVYPGFLQHTGFVAMNPERHAASH
                     WDYYQSLLRGDEDDAEAHRRFYDEYNAVLDMDADYYLDTIRVVFQEFRLAEGTWDVAG
                     ERVRPQDIKKTALFTIEGELDDISGDGQTFAAHELCSGIPEQDKRHFTAEKCGHYGIF
                     SGRRWRTIIYPQLRDFILEHNKTPKVAKEKVEA"
     gene            complement(2229..4109)
                     /locus_tag="VHV99_08960"
     CDS             complement(2229..4109)
                     /locus_tag="VHV99_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX3635604.1"
                     /translation="MPALIEDYALIGDGHTAALISRDGSVDWLCWPRFDSGACFAALL
                     GTPDNGRWLISPVSDTPPTITRRYRGETLILETDFETPEGAVTLIDFMPPGNGWSEMV
                     RIVVGKRGTVRMQMELVLRFDYGFSVPWVDRLKHDSGVKAIVGPDTAVLRTPVELRGE
                     NMKTVAEFTVSEGERIPFSLAYAASHLRIPPARDPHTSLARTENHWLEWSARSTVEGR
                     WAAQIRRSLITLKALAYEPTGGIVAAPTTSLPEQLGGMRNWDYRYCWLRDATITLLAM
                     MRGGYYDEARAWRSWLGRVMAGAPDQLQIMYGIAGERRLPEFEIDWLPGYQDAKPVRI
                     GNNAVGQRQLDVYGEVMNALHLARVGGLQADDTAWNVQRALLGHLDTIWEEPDEGIWE
                     TRGGRQHFTFSKVMAWVAYDRAIQSAEMFKLEGPLDEWRATRAKIHAQVCALAWNPAL
                     NAFSQIYGSDQLDASVLLMPLVGFLPPGDPRVKGTVAAIERDLMHDGFVMRYRTTEYD
                     DGLPPGEGTFLACSFWMVDNLALQGRMDEAIAMYERLLGLCNDVGLLAEEYDPKAQRL
                     VGNFPQAFSHVALVHTGLNLMKHEQRMAQATGQPPHNDTMADEVEAAASPDSSAATSP
                     VP"
     gene            4340..4585
                     /locus_tag="VHV99_08965"
     CDS             4340..4585
                     /locus_tag="VHV99_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635605.1"
                     /translation="MLNPSKSDCITILSAASELADDSMLPLDHGRLGLSRNRMLTAAA
                     FLVERACFRRHQEGDGHYAVGGLSLQGRLRLDQLSNG"
     gene            complement(4876..5028)
                     /locus_tag="VHV99_08970"
     CDS             complement(4876..5028)
                     /locus_tag="VHV99_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3096 domain-containing protein"
                     /protein_id="HEX3635606.1"
                     /translation="MNVTLSLGPLVSLIAGILILVMPRLLNYIVALYLIIIGIIGIFG
                     VGSSHF"
     gene            5350..6645
                     /locus_tag="VHV99_08975"
     CDS             5350..6645
                     /locus_tag="VHV99_08975"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEX3635607.1"
                     /translation="MLRSHPVTARAVSNSSSLSAVQSTSWRGGRPAPRRSARSIIKGF
                     VLMVSIVASHAFAQDSGPAPADGVYDMLVGTYTGGKSEGLYVYRFDTKTGEATRVSVA
                     QTVNPSYLVVSRDRRFVYAVNELPGDNGPATQRGVISAFRFDAASGQLTFLNKVSSAG
                     NDPCYLSLSPDGKYLLTANYSVAADPGGSFAVFPLQADGQVGASVLTVHHEGGGPVKG
                     RQDNSHVHSTVFSPDGHYLFAQDLGADKLYSYRYTPDGSRGLFGPTDWRYTQEKAGTG
                     PRHLVFGADGKHAYLTSELAGTVSTFNYNDGKLTQVQTLSLTEPGFKGAVGAAAIHLS
                     PDGRFVYASNRGDANEIVIFSVDPTNGHLKKIGHQSSLGKSPREFAIDPTGNWLIVGN
                     QNSGTVYVFKRDQQSGLLEANPKRIEIDSPVDFKLVSPS"
     gene            7147..7557
                     /locus_tag="VHV99_08980"
     CDS             7147..7557
                     /locus_tag="VHV99_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635608.1"
                     /translation="MTKITKQLQTASRPPVDALQYEKLALSTFHLCDQQLGQLNRLVN
                     LASTIYRCPTVTRDERQSQRALLALLVETVEGYERDLVRDRELYQVIALDAKGVHESR
                     ITAKHAAGLLANAAIAHAATSASVSVASLNNVDA"
     gene            7694..10324
                     /locus_tag="VHV99_08985"
     CDS             7694..10324
                     /locus_tag="VHV99_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HEX3635609.1"
                     /translation="MRGRLFVPAREPQTDSNTIRFFGIVVYKNISLSLKMRSPSSAKR
                     TTKGRKMKFRTFQGGPASMLCRFLAAFALLLVAFGANATDCFTLYSKSGATPGTKSCR
                     NDVVSNTPGGMGNYACINDIALIDQWCSVPATDEPEQSCPVADPVYPGNGAVTLTEAD
                     FVSGDDMPMSFTRTYRSKPLAKNAISMGPVWFHSWQRSLDLANANSGGSSKVIAYRAN
                     GEPVTFNWLAGTWRTAGFTGLALAQNASGWTLTDLQTETAESYSAQGLLLSEETKTRF
                     IRTATYDASGLLTAITQHAAGTDANKDITLRLDYDDKRRLSRLNDPLGRMTQYGYDAN
                     GNLVSVTWPDGYTHRYAYEDSRFKNALTGEINEAGTRVAAWTYDAQGRAVAVDHPDSA
                     RNVQFAYSTGSTVITASKRTTTLNMSSIGGVLRPTTSTSAAGNASSAWDASGNLLKDT
                     DASGGTSEYSYDETGRPIKATVKGGSATSISTIRYADATSLRPSMIASPNLIQSFVYD
                     ARGNTTGIAETPTSDATGIQGFDAPKPEGAVTTAYGMTYDATNRLSFAQQLAAGKVVA
                     QWVVTRDATGNVFSIYPVEQDVASEAISRDAAHRIEYGYNPTGDYFLRYDRRGRIDLF
                     KFQEDASPANGGVRRILKVRFTYSPDGQVVSRTGTVARNGSLLDLNDGTDIPVGDEEI
                     NQWIDNYNYGDSPIAPPANLQGARRLLGASSLSASTVCGGCHFSAGLIDGAARGIVIV
                     WRLVNNPAVKYGISQGARKAAENWDRIRQMCKPAAETEVDGIPPGRITSEWAEITEGY
                     STTNVATDVTQAEFAANLRNDGWVQTLSKDGKTDIFTKGDLKYTVREDSKSGGPTAEY
                     FPPGAARGTVKIRLNRPRAS"
     gene            10336..10851
                     /locus_tag="VHV99_08990"
     CDS             10336..10851
                     /locus_tag="VHV99_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635610.1"
                     /translation="MSQLDYMHDVTQFTALWERASEGVQRLLDVSPAELLYFDPFDIV
                     TPRLFGLVQTLLAVKGTTDFATVILKPDPFKYFHRHFGKYPGFVHGRNNTYVEFFDLM
                     MQDPGDSPADALGVNYDHYVILPIEGDWIAFGDHPWDTGVFYGPPDIMDCARKFYPYF
                     GTPPSGFRVEL"
BASE COUNT         2243 a         3231 c         3401 g         2289 t
ORIGIN      
        1 cggataacgc ggctcacgaa gacgatgcca ccatgaatca gccgaaaatc aaaagagatc
       61 ctgaaggcac gcggcgccgc attctgcttg cggcggccga agagtttgcg aatggtgggc
      121 tattcggcgc gcgcgtcgat cagatcgccc gccgcgcgga aacgaatgaa cgcatgctct
      181 attactactt cggtagtaaa gagcagcttt tcaccgctgt actcgagcac gcattcagcg
      241 cactcaacga agcggaacgc acgctcgaac tgaatggcgt cgcgccggtc gaggccgtta
      301 cgcgtcttgc gcattttgtt tgggattact accgcgatca tcccgagctg ctgcggctca
      361 tcaacaacga gaacctgcat gaagcacgct acatgcagaa gtcgacgcgc attcgcgaaa
      421 tgatctcgcc gatcgtcgct acgctcggcg gcattcttga acgtggtcaa cgcgccgggc
      481 tgtttcgcac caacgtcgat ccgttgcgct tttacgtgac gctgtccggc atgggctact
      541 acatcgtgtc gaaccgcttt acgctcgaag cgacacttgg ccgcgatttc agcagtgcga
      601 gcgaacgcag tgaagtcatc cagatgaaca ccgagttgct gcttgcgtat ctgttgcgga
      661 aataagctgc gacgcagcgg cgtaacaacg taacaagcgt cataacaagc gctcagaaac
      721 gacaacggcc tctgaagaag aggccgttgt ttatatccgt gcgtgcggtg ctcaggcttc
      781 gactttttcc ttcgctacct tcggcgtctt gttgtgctcg aggatgaagt cgcgcaactg
      841 cggataaatg atggtgcgcc agcggcggcc cgagaaaatg ccgtagtggc cacatttctc
      901 cgcggtgaag tggcgcttgt cctgctccgg aatgccgctg cacaactcgt gtgcggcgaa
      961 tgtctgcccg tcgccggaaa tatcgtccaa ctcgccttcg atcgtgaaga gcgcggtctt
     1021 cttgatgtcc tgcggacgca cccgttcgcc cgcgacgtcc cacgtgcctt cggccaggcg
     1081 gaattcctgg aagaccaccc ggatcgtgtc gaggtaatag tcggcgtcca tgtcgagcac
     1141 ggcgttgtac tcgtcgtaga agcggcgatg ggcttccgcg tcgtcttcgt caccgcgcaa
     1201 caggctctgg tagtagtccc agtgcgaggc cgcgtgccgt tccggattca tcgcgacgaa
     1261 gccggtgtgt tgcaggaagc ccgggtaaac cttgcggccc acgcccggat agttcggcgg
     1321 caccgtgaag atcacgttgt tttcaaacca ctcatacgag tgctgcgtgg cgagcgagtt
     1381 gaccgaggtg gggctcttac gcgcgtcgat cggaccgccc atcatcgtca tcgtgcgcgg
     1441 cgtgtcttcg ccgcggctcg ccatcaacga gatggcggcg agcaccggga ccgtgggctg
     1501 acacaccgag atcacgtgca ggttcttcgc gccgatgtgg cggatgaatt cctgaatgta
     1561 ggcgacgtaa tcgtccagat taaactcgcc ttcttcgagc ggcaccatgc gtgcgtcgat
     1621 ccagtcggtc aggtaaacct tgtggtcttg cagcaacgtg cgcaccgtat cgcgcagcag
     1681 cgtggcgtgg tgaccggaca acggcgcaca caccagcacg ataggctcgt ctttcaattg
     1741 gataacagcg tcgctgtcgt cggcgaaacg cttgaagtgc atcaggcggc aaaacggctt
     1801 ttcgataatc gtctgttcga tgatcggaat gttatgaccg tctttaacaa tctgatgaag
     1861 attgaactcg ggcttttcgt aatctttgcc gagccggtag agcagttcat aaccggcgga
     1921 cagacgtgtg gcgccgggca cataggccaa tgggctggcg ggattcgcga acgatttcga
     1981 cgcggcctgg gcccaggcgg tgagagggct caacatcgcc cgttggaatt cgtgcagttg
     2041 gtagagcatg ggtgctccgt ttaagcggtt cgcatggggc ttggcaaaca cgcgtgtggc
     2101 gttgcggcag gccgtattgg ttgtgggcac attgattttc ggccaacagt ctgctcgatc
     2161 atactgaaca aagcgtactt gtgcaatgca gcaatttgcg cgcgggctgc gcccaatccg
     2221 tcattaaatc atggtactgg cgatgttgcc gcgctgctat cggggcttgc cgcagcctca
     2281 acttcatccg ccatggtgtc gttatgcggc ggctgcccgg tcgcctgggc catccgctgt
     2341 tcgtgtttca tcaggttcag gccggtgtgc acgagcgcca catgcgaaaa cgcctgcggg
     2401 aagttgccga cgaggcgctg cgccttcggg tcgtactctt ccgcgagcag accgacgtca
     2461 ttgcacagac cgagcaggcg ttcatacatg gcgatggctt catccatgcg gccctgcagt
     2521 gccaggttgt ccaccatcca gaacgagcac gcaaggaagg tgccttcgcc gggcggcagg
     2581 ccgtcgtcgt actcggtggt gcggtagcgc atcacgaaac cgtcatgcat caggtccctt
     2641 tcgattgccg ccaccgtgcc tttgacgcgc ggatcgcccg gcggcaggaa gccgaccagc
     2701 ggcatcagca gtacgctcgc atcgagctgg tcgctaccgt agatctggga gaacgcgttg
     2761 agcgccggat tccatgccag cgcacagact tgcgcgtgga ttttcgcgcg ggtggcgcgc
     2821 cattcatcga gcggaccttc cagcttgaac atttcggccg actgaatggc gcggtcgtag
     2881 gcgacccacg ccatgacctt cgagaaggtg aagtgctggc ggccgccgcg ggtttcccag
     2941 atgccttcgt ccggctcttc ccagatcgtg tcgaggtggc cgagcagcgc gcgctgcacg
     3001 ttccatgcgg tgtcgtccgc ctgcaagccg ccgacgcgcg cgagatgcag cgcgttcatc
     3061 acttcgccat agacgtcgag ctgacgctgg ccgaccgcgt tgttgccgat ccgcacgggt
     3121 ttggcatcct gatagcccgg cagccagtcg atttcgaact cgggcaggcg ccgctcgccg
     3181 gcgatgccgt acatgatctg cagttgatcc ggcgcgccgg ccatgacccg gccgagccag
     3241 ctgcgccacg cgcgcgcttc gtcgtagtag ccgccgcgca tcattgcgag cagcgtgatg
     3301 gtggcgtcgc gcagccagca gtagcggtag tcccagttgc gcatgccgcc cagctgttcc
     3361 ggcagcgacg tggtgggcgc ggcgacgatg ccgccggtcg gctcgtacgc gagcgccttc
     3421 agcgtgatca gcgagcggcg gatctgcgcg gcccatctgc cttcgaccgt gctgcgcgcc
     3481 gaccattcga gccaatggtt ctcggtgcgt gcgagcgacg tgtgcggatc gcgcgcgggg
     3541 ggaatgcgca gatgcgaagc ggcataagcc agcgaaaacg gtatgcgttc gccttcgctg
     3601 acggtgaact cggcgacggt cttcatgttc tcgccgcgta gctcgaccgg cgtgcgcagc
     3661 accgcggtgt ccgggccgac gatcgccttg acgccgctgt cgtgcttcaa acgatcgacc
     3721 cacggaaccg aaaagccgta gtcgaagcgc agcaccagtt ccatctgcat gcgcacggtg
     3781 ccgcgtttgc cgaccacgat ccgcaccatc tccgaccagc cgttgcccgg cggcatgaag
     3841 tcgatcagcg tgacggcgcc ttcgggtgtt tcgaaatcgg tttccagaat cagcgtttcg
     3901 ccgcgatacc ggcgggtgat ggtgggcggc gtatccgaaa ccggcgaaat cagccagcgg
     3961 ccgttgtcgg gcgtgccgag cagggcggcg aaacaggcgc cggagtcgaa tcgcggccag
     4021 caaagccagt cgaccgaccc gtcccgggag atgagcgcgg ccgtgtggcc gtcgccgatg
     4081 agtgcgtagt cttcgatgag tgcgggcatg gaggagcgtg tcagtgttac gcgaggcgaa
     4141 ggccgccacg cgtggggtca ttgttattac cggactcgcg tgaggcgctt tggttcagca
     4201 cgcaatgggg caaacgcggg ttggtaattg tcaaatcact gcctacaatc gattttcgtg
     4261 ccgatgcgcg gagccatccg gatcatgcat cggccttggc tcacggcgcg tgcaaacacc
     4321 gttttcgagg atccaagcaa tgctgaaccc gtcgaaatcc gattgcatca caattctctc
     4381 ggccgccagc gaactggccg acgactcaat gttgccgctc gatcatggcc ggctgggcct
     4441 gagccgcaac cgcatgctga ccgcggcggc ctttctggtc gaacgggcct gcttccggcg
     4501 tcatcaggaa ggtgacggtc attatgccgt cggcggattg tcattgcagg ggcgtttgcg
     4561 gctcgatcag ctttcgaacg gctgactgtc ggttgcgcgg gctcggcgcc gcgcggcggt
     4621 cttccatctg gtgcgcagca tggggtgcga atctgtcgcg cgtatgcata ttgccgcgcg
     4681 ctgcattgtc gctccgtttc gcttttccgt tcagtctcgc tgcttccttg tgctacttgc
     4741 tgcgccaccg cggtcacgtc gatacgcgtg ccgttgctca tctaccgtcg cgttcatgcg
     4801 gggggcgccg ggccggcatt gcgcccggtc aagagtcctt actgctttgt tactgactgt
     4861 cgccgccgct tgggcttaga agtgcgacga ccccacgccg aagatcccaa tgatgccgat
     4921 gatgatcaga tagagcgcga cgatgtagtt caacaggcgc ggcatgacga gaatcagaat
     4981 accggcgatc aacgaaacga gcgggcccag gctgagtgtg acgttcatga agactccgta
     5041 gtgtgtaatg ggtgagacgc ggttgaaaaa atttcccgcc gggcgggacg attgctatgc
     5101 gtgcagcggg atgccggcgg cccgagcgcg gccgggccga atccggtgag ccgatcgcgg
     5161 tgcggtccaa tgtagcccac ccgagcacga cgcggcccgg cccggccaac cgcaaccggc
     5221 cggaatcgct gcatgatccg acgcaacgct tttatacttg gctcgacaaa acaattcgac
     5281 cgaatgcgcg gcagacgaga gacaccatta caaccgattc aaccgaataa ccgatgtaga
     5341 ggaggtcata tgcttcgtag tcatccggtc acagcgcgcg cagtttcaaa ctcttcatca
     5401 ctttctgctg ttcaatccac ctcatggcgc ggcggccggc cagctcctcg gcgctccgct
     5461 cgctcgataa taaaaggttt cgtgctcatg gtctcgattg ttgcttcgca tgcgtttgcg
     5521 caagattccg gcccggctcc cgccgacggc gtctatgaca tgctggtcgg cacttatacc
     5581 ggtggcaaga gcgaaggcct atacgtctac cgcttcgaca cgaagacggg cgaagcgacc
     5641 cgcgtctcgg tcgcgcagac ggtcaacccg tcgtatttgg tggtgagccg cgatcgccgc
     5701 ttcgtctacg cggtcaacga attgcccggc gacaacggcc cggccacgca acgcggcgtc
     5761 atcagcgcgt ttcgcttcga tgccgcaagc ggccagttga cctttctcaa caaggtctcg
     5821 tcggccggca atgacccgtg ctatctgagc ctgtcgccgg acggcaagta tctgctgacg
     5881 gccaactact cggtggcggc cgatccgggc ggcagcttcg cggtgttccc gttgcaggcg
     5941 gatggccagg tgggcgcgtc tgtgctcacg gtgcaccatg aaggcggcgg tccggtgaag
     6001 ggacgtcagg acaactcgca tgttcactcc accgttttct cgccggacgg tcactatctg
     6061 ttcgcgcaag atctaggcgc cgacaagctg tactcatacc gctacacgcc tgacggcagc
     6121 cgcggcctgt tcggcccgac cgactggcgc tacacccagg agaaagcggg caccggcccg
     6181 cgtcatctgg tgttcggcgc ggacggcaag catgcgtatc tgacgagcga actggcgggc
     6241 acggtcagca ccttcaacta caacgacggc aagctcacgc aagtgcagac gctttcgttg
     6301 acggaacctg gcttcaaggg cgcggtgggc gccgcggcga ttcatctgtc gccggacgga
     6361 cgctttgtct acgcgtcgaa tcgcggcgac gcgaacgaga tcgtgatctt ctcggtcgac
     6421 cccaccaatg gtcatctgaa gaagatcggg caccagtcga gcctcggcaa gtcgccgcgc
     6481 gaattcgcga tcgacccgac cggcaactgg ctgatcgtcg gcaatcagaa tagcggcacg
     6541 gtgtatgtgt tcaagcgcga tcagcagagc ggcttgcttg aggcgaatcc gaagcggatc
     6601 gagatcgatt cgccggtgga tttcaagttg gtgtcgcctt cgtgaggggc gcgtgtcggc
     6661 gccgcattta gaagatgggt cgcttcgggc aatactgttt ttgagtgaac ccgtatggcg
     6721 atgatgacag cgtgcttcca agtcgttgcg gccgcttggg gcaatgcttc cgtcgaggcg
     6781 gtccgggaca gcatttaatc aacgtttcgg aagtttaaga aagaaatttc tgaaataata
     6841 tcgaccgggc cgattttgcc tacgcgggta agcgcgtgct acatcgtgcg gcttatactc
     6901 agaaagccgt cgaaacagcg acggttcagg cttggcagct tgagttactg aaacccgcgc
     6961 gcctgcttcg gcaggtcgtg cgtttgcttc cgcgcgcctg tctttccatg ggcgggctgt
     7021 agcaaggaga ccttcgggtc tgccggtttg tggtttcagt accggtctgc caaccttgtt
     7081 acgtgcccgc tcaccctatc tgttagcaag tgtcgggcga ttcaggaacg aaccggaaat
     7141 cgcaccatga caaagatcac caaacagctt caaaccgcgt cacgtccccc ggtcgacgca
     7201 cttcagtacg agaaactcgc gctttcaact ttccacttat gcgatcagca acttggccag
     7261 cttaatcggc tggttaatct ggcaagcaca atctatcgat gcccgaccgt gacacgcgac
     7321 gaacggcaaa gccaacgcgc attgctggca ctcctagtgg agacggtcga aggatatgag
     7381 cgtgatctcg tgcgtgatcg cgagttgtat caggtcattg cgctcgatgc taaaggcgta
     7441 catgaaagcc gcatcaccgc gaagcatgca gcgggtctgc tcgccaatgc ggcaatagca
     7501 catgctgcaa cgagcgcaag cgtgtctgtc gcatcgctaa acaatgttga cgcatagcgc
     7561 ataaccatgc ggtagtcgct gtagcgttgc gagggctttg tgcccctgtg agaaacaaag
     7621 gcgtgaagcg atccacgcct ttttctcatc gccacgttca tctctgtggt ggtagctcgg
     7681 tcatgcacgg tcagtgcgag gccgtctctt cgttcctgcc agggaaccgc agactgacag
     7741 caacacaatc agatttttcg gaattgtcgt atataaaaat atttcactct ccttaaaaat
     7801 gcgttcgccc tcatcggcga aacgaacaac taaagggaga aaaatgaaat tccggacatt
     7861 ccagggcggc ccggccagca tgctgtgccg cttcctcgcc gcattcgcgt tgttactggt
     7921 ggcgttcggc gcaaacgcga cagactgctt cacgctgtac tcaaagtcgg gcgcaacgcc
     7981 gggtacgaag tcctgccgca acgatgtcgt cagcaataca cccggtggca tgggaaatta
     8041 cgcgtgcatc aacgacatcg cgctgatcga ccaatggtgt tccgttccag cgacggatga
     8101 gcccgaacaa agctgcccgg tagccgatcc ggtttatccc ggcaatggcg cggtcacgct
     8161 gacggaagcg gatttcgtca gcggcgacga tatgccgatg tcgttcacgc gcacctatcg
     8221 ctccaaacca cttgcgaaga atgcgatctc gatgggaccg gtctggttcc atagctggca
     8281 gcgcagcctg gacctcgcga atgcgaacag cggtggttca tccaaggtta ttgcgtatcg
     8341 cgcgaatggt gagcctgtca cgttcaactg gttggcgggc acttggcgta cggctggctt
     8401 tactggtctc gcgcttgcgc agaacgcgtc cggctggacg cttactgatc ttcagacgga
     8461 gacagccgaa tcgtactcgg cgcaagggct cttgctgtcg gaggagacga agacccggtt
     8521 tatccgcacg gcaacctacg atgcatcggg tctgctaaca gctatcactc agcatgctgc
     8581 gggcacggac gcgaacaagg acattacgct tcgactcgac tatgacgaca agcgtcgtct
     8641 gtcgcgcctg aacgatcctt tgggacgtat gacgcaatac ggttatgacg cgaatggcaa
     8701 tctcgtttcc gttacgtggc cggatggtta cacacaccgt tacgcctatg aagattcccg
     8761 gttcaagaat gcgctgacgg gagagatcaa cgaagccggc acgcgtgtcg cggcgtggac
     8821 ttatgacgcg caaggtcggg cagtagcggt cgaccatcct gactctgcac gcaatgtgca
     8881 gttcgcctat agcaccggct cgacggttat taccgccagc aagcgcacca ctacgttgaa
     8941 catgtcgtcg attggcggcg tgctgcggcc aaccactagc acgtcggcgg cgggaaatgc
     9001 gagcagcgca tgggatgcgt cgggcaatct gctgaaagac accgacgcaa gcggtggaac
     9061 gtcggaatac agctacgacg aaaccgggcg tccgatcaaa gcgaccgtta agggcggctc
     9121 ggcgacttcg atatcgacga ttcgctatgc cgatgcgacg agcctgcgtc cgtcgatgat
     9181 tgcgtcgccg aacctgattc agtcattcgt ctacgacgct cgcggcaaca cgacaggcat
     9241 cgccgagacg ccgacgagcg acgcgaccgg aatccagggc ttcgatgcac cgaagcctga
     9301 aggcgccgtg acgacggcct acggcatgac gtacgacgcg acgaaccggc tgtcgtttgc
     9361 ccagcaactt gccgccggga aggtcgtcgc tcaatgggtc gtgacgcgcg atgcgaccgg
     9421 gaatgtattt tcaatctatc ccgtggagca agacgtcgcc agtgaggcga tttcacgcga
     9481 tgccgctcac cggatcgagt acggctacaa cccgactggc gactatttcc tgcgctatga
     9541 ccggcgcgga cgaatcgacc tcttcaagtt tcaggaggat gcaagtcctg cgaacggtgg
     9601 tgtccggcgc attttaaaag tcaggtttac gtattcgccc gatggtcagg tcgtgtcacg
     9661 tacgggcacg gttgccagaa acggcagtct gctcgacctg aacgacggta cggatattcc
     9721 ggtcggcgat gaggaaatca atcagtggat tgataactac aactatgggg actcgcccat
     9781 agcgccaccg gcaaatcttc agggcgcacg tcggcttctc ggtgcttctt cgctttcggc
     9841 ttcgactgtg tgtggtggat gccatttctc ggcggggctg attgacggcg cggcacgggg
     9901 aatcgtgatt gtctggcgac tcgtcaataa tccggctgtc aaatacggca tcagtcaagg
     9961 cgcaaggaaa gcggcggaga actgggaccg tatcaggcag atgtgcaagc ctgcggctga
    10021 gacggaggtg gatgggattc cgccggggag gattacgtcg gaatgggcgg agataactga
    10081 agggtatagt acgacgaatg tcgcaaccga cgtaacgcaa gcagaatttg cagccaacct
    10141 acgcaatgac ggatgggtgc agacgctaag taaggatggc aaaaccgaca tttttacaaa
    10201 gggcgacctc aaatatacgg tgcgcgagga ttcaaaatct ggcggaccta ccgccgaata
    10261 ttttccgcct ggcgccgcga ggggcaccgt taagattcga ctgaatagac ctagagcgtc
    10321 ttaaggtacc taatcatgag tcaacttgat tacatgcatg atgtcactca gttcacggcg
    10381 ttatgggagc gcgccagtga aggcgtgcaa cgccttctgg atgtctcgcc tgccgaactg
    10441 ctttacttcg atccgttcga cattgttacg ccacgtttgt tcggactggt acagacgtta
    10501 cttgccgtca agggcacgac cgactttgca acggtgattt tgaaaccgga cccgttcaag
    10561 tattttcacc gtcactttgg gaaatatcca ggcttcgtgc atggacggaa caatacttac
    10621 gtcgaatttt tcgatttgat gatgcaggat cctggcgaca gtccggctga tgcactcggc
    10681 gtgaactacg accactacgt gatattgccg atagaaggtg actggatcgc cttcggggac
    10741 cacccgtggg ataccggtgt cttctacgga ccacccgaca ttatggactg cgcacgtaag
    10801 ttctatccgt atttcggcac gccgccgagc ggctttcgcg tcgagctgtg ataactcgaa
    10861 agagcggtca tcgagaaggt tgcccgaacg agtgcactca aaaaagcgcg agcaatttgc
    10921 gcctttttcc tcagtgcaca cgcatgcgac caatagggtg tgggcaccct tattgttcgg
    10981 tggccctacc gccgaattcg accgcatcaa gacaaccaga catcctttcc aagggggaaa
    11041 accagctcac tgtccgtgag cagtcaaact ctggatggat cactgccgat gtcaagcgca
    11101 gtggcagcta cagcaaaaat tagcttaggt gggaaatgac tcgtcaacat aaaagcaatt
    11161 tttt
//
LOCUS       DATASH010000319         3023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4791384,
            whole genome shotgun sequence.
ACCESSION   DATASH010000319 DATASH010000000
VERSION     DATASH010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4791384"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VHV99_08995"
     CDS             <1..292
                     /locus_tag="VHV99_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008163976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX3635611.1"
                     /translation="HSGSTHETVTTTRLAKEAGARTICITNFGRSPIQAFADIVLFTM
                     ARETKFRTEAMTSRLAQLAIIDVLIACLALSDYERSAETIRRTFDVLSLKRY"
     gene            complement(342..1739)
                     /locus_tag="VHV99_09000"
     CDS             complement(342..1739)
                     /locus_tag="VHV99_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007733111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3635612.1"
                     /translation="MKLRLWPDTLYGRLVLILVVGMFAGQLLTSTIWFETHDSRTLEI
                     PARLFASRLADTVRLLQHAPDDASRREIATQLGDARYRLQWLDAPAPAPAGSVARQAV
                     GDLISGVMRRRLGEPIEVRLIDAELRGDAGQHAGILSLFNSRMPTGDFHVQMKLPQGE
                     WLDVQANEGQAGMQIEPHALVLDYLTRIYLLRLAAVVLLALVAVRFAVRPLRQLAKAA
                     DALGRNIYRPPLPVSGPREVRTAAQSFNAMQSQLIDGISARTRFLTAVSHDLRSPLTR
                     LRLRAEMLPDPTWRESMRGDLEDMEAMVRATLDAVQGVEITEQRHEIDIDSMIEGLAE
                     DAREAGHVVDVEGRAVHPLSGYPRNLKRCLQNLVDNGIYYGGRVKIRVTDLEHAVQIV
                     VSDSGPGIPDETMLERVFEAYFRIDASKDGKSQGTGLGLTIARSIAAAHGGTLTLRNR
                     ADRGLDAVLTLNRDD"
     gene            complement(1753..2466)
                     /locus_tag="VHV99_09005"
     CDS             complement(1753..2466)
                     /locus_tag="VHV99_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013691224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3635613.1"
                     /translation="MDHILVVDDDPNIRQLLRDYLHDMGYTASAAENGAQMKQIINKS
                     RVDLLVLDLMLNGEDGLDITRELRREHSMPIIILSARGGLLDRILGLEMGADDYLPKP
                     FYPRELVAKIKVVLRRTRSVPGARQADSLAFVSFAGWRLDTRMKQMLSPDGVIVSLGG
                     SDYRALRALLDHPNRPLSREFLLDQVFGKERTPLDRSIDVCVSRLRHYLKDEARGAKL
                     IRTVRNEGYMLVADVVYEA"
     gene            2618..>3023
                     /locus_tag="VHV99_09010"
     CDS             2618..>3023
                     /locus_tag="VHV99_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011349792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HEX3635614.1"
                     /translation="MAIILISNYIMSILRFSLARRPLTRAAGAFAACVALTFGAVVTS
                     TGACAATQAPASHAAQSVTDLAGRTVAIPAEPHRILLGESRLLTAVALLEGQHPLARI
                     VGWQGDLPTMDPQTYDAYAKKFPEIKQIPLIGK"
BASE COUNT          572 a         1008 c          891 g          552 t
ORIGIN      
        1 gcactcgggc agcacgcacg aaacggtgac gacaacgcgc ctcgccaagg aagccggcgc
       61 gcgcaccatc tgcatcacga acttcgggcg ttcgccgatt caggcctttg ccgatatcgt
      121 gctgttcacc atggcgcgcg agaccaaatt tcgcacggaa gccatgacga gccgtctcgc
      181 acaactggcg atcatcgacg tgctgattgc gtgcctcgcg ctctcggact acgagagatc
      241 ggcggaaacc atccgccgca cgttcgacgt gctgtcgctt aagcgatatt gacggccacg
      301 ggcagcgccg gtgcgatgca attccaggcc tcacggtctg cctagtcgtc ccgattcaaa
      361 gtcagcaccg cgtccagacc tctgtccgcg cggtttctca gcgtgagggt tccgccatgt
      421 gcggcagcaa tgctccgcgc aattgtcagc cccaaacctg taccctggct tttcccgtct
      481 tttgacgcat cgatcctgaa gtacgcttcg aacacccgtt caagcatggt ctcgtcggga
      541 ataccaggac cgctgtcgct cacgacgatt tgcacagcgt gctccagatc ggtcacccgg
      601 atcttgactc gaccgccgta gtagatcccg ttatccacca gattctgcaa acagcgcttg
      661 agattgcgcg gatacccact caatggatgc accgcgcgcc cctcgacatc caccacatgt
      721 cccgcctcac gcgcatcctc agccagccct tcgatcatcg aatcgatatc gatctcatga
      781 cgctgctcgg tgatctcaac gccttgaacc gcgtccagcg tcgcgcgcac catggcttcc
      841 atgtcttcga gatcgccacg catggattcc cgccatgtcg gatcaggcag catttcggcg
      901 cgcagccgca aacgcgtcag cggcgagcgc agatcatgtg aaacggccgt tagaaagcgc
      961 gtgcgggcgc tgatgccgtc gatcaactgg ctctgcatgg cattgaacga ttgcgccgcg
     1021 gtgcgcactt cccgtgggcc actaaccggc agcgggggcc gatagatatt gcgaccaagc
     1081 gcgtccgcgg ccttcgcgag ctgccgcaac ggacgcaccg caaagcgcac cgccaccaga
     1141 gccaacagca ccacggccgc caggcgaagc agatagatcc gcgtgaggta gtcgagcacg
     1201 agcgcgtgcg gctcgatctg catccccgcc tgcccctcgt tggcctgcac gtcgagccac
     1261 tcaccctgcg gcagtttcat ctgcacatga aaatcgcccg ttggcatacg cgagttgaac
     1321 aggctgagga tgcccgcgtg ctggcccgcg tcgccacgca gttcggcgtc gatcaggcgc
     1381 acttcgatcg gctcgcccag acggcggcgc atcacaccgg agatcagatc gccgacggct
     1441 tgcctcgcga ccgatccagc gggcgccgga gccggcgcat cgagccactg caaccggtag
     1501 cgcgcatcgc ctagctgcgt ggcaatttca cggcgcgaag cgtcatcggg cgcatgctga
     1561 agcagccgca cggtatcggc aagacggctc gcaaagagac gcgcgggaat ttcaagggtg
     1621 cggctgtcgt gggtctcgaa ccagatagtg ctcgtcaaaa gttgtccggc aaacatgccg
     1681 actaccagaa tcaggacaag ccgcccatac agggtgtcgg gccagaggcg cagtttcatc
     1741 gctggtcgcg cctcatgctt cgtagacgac gtcggcaacc agcatgtagc cctcgttgcg
     1801 caccgtgcga atcagcttcg ccccacgcgc ctcgtctttc agatagtgcc gcagacggct
     1861 cacgcagacg tcgatcgagc ggtcgagcgg cgtgcgctcc ttgccgaata cctggtcgag
     1921 caggaactcg cgcgacagcg gccggttcgg atgatccagc agcgcgcgca gcgcgcgata
     1981 gtcggagccg ccgagcgaga cgatcacgcc gtcgggcgac agcatctgct tcatgcgcgt
     2041 atcgagccgc caaccggcaa agctcacgaa cgcgagcgaa tcggcctgac gcgcaccggg
     2101 cacgctgcgg gtgcgcctca gcactacctt gatcttcgcg acgagttcgc gtgggtagaa
     2161 aggcttcggc agatagtcgt ccgcgcccat ctcgaggccg aggatccggt cgagcaaacc
     2221 gccgcgcgcg ctcaggatga tgatcggcat gctgtgctcg cgccgcagtt cgcgggtgat
     2281 gtcgagacca tcctccccgt tcagcatcag gtcgagcacc agcagatcca cgcgcgactt
     2341 attgatgatc tgcttcatct gtgcgccgtt ttcagcggcg ctagccgtat agcccatgtc
     2401 gtgcagatag tcgcgcagca actggcggat gttgggatcg tcgtcgacga cgagtatgtg
     2461 gtccatcgtg aaagcctttt ttcccggcac cggctgcgtt gagccggtgt ttgcgtggcc
     2521 ccgtgcagag ccgcgcccgt ctcaacacat ttcattacaa aacggccgtg tttcaacaca
     2581 ttgccattac atcgccccgc taaagtaagg tccgcaaatg gcaatcattc tcatttcgaa
     2641 ctatatcatg tcgattctcc ggttttctct ggctcgccgg cccttgacgc gtgcggctgg
     2701 cgcatttgcg gcctgcgtcg cgctcacctt tggcgcggtc gtcacgagca cgggcgcgtg
     2761 cgccgctacg caggcgccgg ccagccacgc agcccagtcg gtcactgacc tcgcgggccg
     2821 caccgtcgcg attcccgccg agccgcaccg tatcctgctg ggtgaaagcc ggttgctcac
     2881 ggcggtggcc ctgcttgaag gtcagcatcc gctggcacgc atcgttggct ggcaaggcga
     2941 tctgccgacc atggacccgc aaacctacga cgcctacgcg aagaagtttc cggagatcaa
     3001 acagatcccg ctaatcggca agg
//
LOCUS       DATASH010000320         5749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3211613,
            whole genome shotgun sequence.
ACCESSION   DATASH010000320 DATASH010000000
VERSION     DATASH010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5749
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3211613"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="VHV99_09015"
     CDS             <1..707
                     /locus_tag="VHV99_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEX3635615.1"
                     /translation="FKGFAALQFVVALAGCTLCAGNSYAQASIDTSTFVGVPLTKTVR
                     SMRDIRYNHIVNQQFDYSCGAAALATLLKYGYGIDIPETELIRRMMVFSTPEVVVKNG
                     FSMLDMKKFVETIGLRGRGFRVNADALQHLQIPVMVLMNIDGYEHFVIVKHAQDGRVF
                     IADPALGNRIVLEEDFAKTWNGLVFAVLGKPFREDSPLLQDNESLALKLRANALANGT
                     AATPFVEYGLIKADLF"
     gene            715..1527
                     /locus_tag="VHV99_09020"
     CDS             715..1527
                     /locus_tag="VHV99_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HEX3635616.1"
                     /translation="MKRHLSQLGLVICTTMCGLSSSAYASQGAGSPPVPEFLAAHDID
                     SAAIKVQMVDDDVLAHQTGKYAGATMISGFVLNVISQWQLPNGVTALAQGTLAAAQNA
                     AGQVSSSVNTFASVTGGGPGSSGANSGANPNAQANGGQSISVNGVSQVTQVAGDRNAG
                     SNSALIDFNSNAVTLAGTANAPSAAASNSTGSIKAGISFGSNGINVALRTPAGLATQT
                     ITPGNGQIAQLLQIAGNNQQVANALQLHLQTQQMSAAMIRQLGVLQALQNRR"
     gene            1641..3089
                     /locus_tag="VHV99_09025"
     CDS             1641..3089
                     /locus_tag="VHV99_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635617.1"
                     /translation="MNNFSSTKVPRMALAAIPAAVMLFALSPGASAQALAGQSVEDRL
                     NTLMRVVDEQQRQIHSLERQVTNLEMAQRGRGMPGYGAPAGSETVAEQPGADGLPVPL
                     PPLAQISPGAPATGGNTGTATGVPVYPPPPENGNASNSAGGLPAATDGSGAIGQTQKA
                     AEPVRTQAEEAVVQREHAPLFDHKLTMDWGISDTYYDRHQLQLSGFLALDAIFLGNIN
                     LGETKSHQVMADLDTRYGLTDRMSIDVDVPYIYRHSNFIVGGAGGAASTLSDASVNGS
                     DIGDVNFGIYYQFLKETNSLPDVVGSLRIKAPTGSSPFGDKLIQVDNDNTNLVAPSKL
                     PTGTGFWNITAGISLLKTYDPVVLFGSLSYTYNIARSFADISSVQGQTQPATVKLGDI
                     VQFGGGVALAFSDKDSASISYTMALEPESKTRAPGGSYEKVPGSETTAAALNFGLNHV
                     VNKHLTINGSVSVGLTPDAPNFVVGVRFPYTF"
     gene            3184..4755
                     /locus_tag="VHV99_09030"
     CDS             3184..4755
                     /locus_tag="VHV99_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX3635618.1"
                     /translation="MRESPHLTSVASVAGAGSHMTRTLPADRPVEAVTKCAAVAAVVS
                     IGAPAAGASGTPCAHARAARMLLYVARTPDDTLVAHLRSRGWNVAVARSAHELGRLVK
                     PDAACAGIVDLASFPPRDLGGLEASLRQQQVGWIALATNERLNDPLARRLVRHYCFDY
                     VKIPVANATVDYLVSHAFGMATLGEPELAAPVNESGDEEMVGTCEAMQQLFRTIRKVA
                     NTDASVFISGESGTGKELTALAIHERSPRRKTPFIAINCGAIPHHLLQSELFGYERGA
                     FTGANQRKIGRVEAADGGTLLLDEIGDLPLESQASLLRFLQEGKIERLGGRESIPVDV
                     RIISATHVDLEGAMRAGQFRADLFHRLCVLRVDEPPLRARGKDIEILAHHVLHKFKTD
                     SARKIRGFTPSAIEALYSYSWPGNVRELINRVRRAIVMAESKLISADDLDLAQFTEQQ
                     TMSLSQARDAAEKRAIEAALLRHRHRLNEAAADLNISRVTLYRLMGHHGLRELSAADE
                     KASLESFAAEDPSQK"
     gene            5022..5522
                     /locus_tag="VHV99_09035"
     CDS             5022..5522
                     /locus_tag="VHV99_09035"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEX3635619.1"
                     /translation="MTTLTLIVARANNGVIGRDNQLPWRLPEDLAFFKRTTMGAPIIM
                     GRKTHESIGRPLPGRRNIVVTRDATRRFQGCDAATTLEDALALAAQDQAPEAFLIGGA
                     QLYAEGLRQADKLIITEISADFEGDATFPELDENEWEEVAHETHRADAPNDFDYAFVT
                     YRRKGS"
BASE COUNT         1095 a         1816 c         1815 g         1023 t
ORIGIN      
        1 ggttcaaggg gttcgcggcg ctacagttcg ttgtagcgct ggcaggttgc acgctgtgtg
       61 ccggtaacag ctacgcacag gcgagcatcg acacgtccac tttcgtgggc gttccgttga
      121 ccaagacggt gcgctcgatg cgggacatcc ggtacaacca catcgtcaat cagcagttcg
      181 attacagctg cggtgcggcg gcgctagcga ccctcctcaa atacggctat ggcatcgaca
      241 ttccggagac cgaactgatc cgccggatga tggtcttctc gacgcccgaa gtcgtcgtca
      301 aaaatggctt ctcgatgctc gacatgaaga agttcgtcga gaccatcggt ttgcgcggcc
      361 gtggcttccg ggtcaacgcc gacgcgctcc aacacttgca aatccccgtg atggttctga
      421 tgaacatcga cgggtacgag catttcgtga tcgtcaaaca tgcgcaggat ggccgcgtct
      481 tcattgcgga tccggcgctc ggcaatcgca tcgtgctcga agaggacttc gccaaaacct
      541 ggaacggcct ggtgttcgcg gtcctcggca aacccttcag ggaggattca ccgctgttgc
      601 aggacaacga gtcgctcgcg ctcaagctgc gcgccaacgc gctggccaac ggcacggcgg
      661 cgactccctt tgtcgaatac ggcttgatca aggcagacct gttctgaccc aatcatgaag
      721 cgccaccttt cccagctagg cctcgtaatc tgcaccacga tgtgcggcct gagcagcagc
      781 gcctatgcat cgcaaggcgc cggctcgccg ccggtgcccg aatttctcgc cgctcacgac
      841 atcgactccg ccgccatcaa ggtgcagatg gtggacgacg atgttctcgc ccaccagacc
      901 ggcaaatatg ccggcgccac gatgatttcc ggcttcgttc tgaacgtcat ttcgcaatgg
      961 caattgccca acggggtgac cgccctcgct caagggacgc ttgctgccgc acagaacgct
     1021 gccggacagg tgagcagcag cgtcaacacg tttgcgagcg tgactggcgg cggccccggc
     1081 agttccggcg ccaactcggg ggccaacccc aacgcgcagg caaacggcgg acaaagcatt
     1141 tcggtcaacg gcgtttcgca ggtcacgcaa gtggctggcg accggaacgc cgggtccaat
     1201 tccgcgctga ttgatttcaa tagtaatgcc gtgacgctgg cgggtaccgc caatgccccg
     1261 tcagcagcgg ccagcaattc gaccggctcg atcaaagccg gcatctcgtt cggcagcaac
     1321 ggtatcaacg ttgcgctgcg gactccggcc gggctcgcca cccagaccat cacgccaggc
     1381 aacgggcaaa tcgcccagtt gctgcagatt gcaggcaata accagcaagt cgccaacgca
     1441 ttgcagttgc atctgcagac gcagcagatg tcggcggcaa tgattcgtca gctcggcgta
     1501 ctacaagctc tgcaaaacag gagatgaagg agatgaccct gcgggccgcc acccgcgcaa
     1561 ggcagcacat gcagtactga tacagcagcg cgtcgccggg agacagatgg cgcgccgcag
     1621 gaacaccggg ggaacacaaa atgaataatt tctcatcgac aaaggtgccg cgcatggcac
     1681 tggcggccat tccggccgcc gtcatgctgt tcgcactttc gccgggggcg agcgcgcagg
     1741 cactcgcggg acaatccgtc gaggaccgtc tcaatacgtt gatgcgggta gtcgacgagc
     1801 agcagcggca aattcattca ctcgagcggc aggtcaccaa tctggaaatg gcgcaacgcg
     1861 gccgcggcat gccgggctac ggtgcgccgg caggcagcga aaccgtggcc gaacagccag
     1921 gcgccgatgg cctgccggtg ccgctgccgc cgctcgccca gatcagtccc ggcgcgcccg
     1981 cgacgggcgg caacacgggc acggcgaccg gcgtgcctgt ctatccgccg ccgcccgaaa
     2041 acggcaatgc gagtaacagc gcaggcggtt tgccggccgc caccgacggc agcggcgcga
     2101 tcggccagac ccagaaagcc gccgaaccgg tgcgcaccca agccgaagaa gccgtcgtgc
     2161 agcgcgaaca tgcgccgctc ttcgaccaca agctgacgat ggactggggt atcagcgata
     2221 cctactacga tcgacatcag ctgcagttgt cgggcttcct cgcgctcgac gcgattttcc
     2281 tcggcaacat caacctgggc gagaccaagt cgcaccaggt gatggccgat ctcgatacgc
     2341 gctacggcct gaccgaccgc atgagtatcg atgtcgacgt accgtacatc taccggcata
     2401 gcaacttcat cgtcggcggt gcgggcggtg cggcgagcac gttgtcggac gcgtcggtga
     2461 acggcagcga tatcggcgac gtcaacttcg ggatctacta ccagttcctg aaagagacca
     2521 atagtctgcc ggacgtggtc ggcagcctgc gcatcaaggc gccgaccggt tcgtcgccgt
     2581 tcggcgacaa gctgattcag gtcgacaacg acaataccaa cctcgttgcg ccgagcaagc
     2641 tgcccaccgg aaccggcttc tggaacatca cggccggcat ctcgctgttg aagacctatg
     2701 acccggtggt gctgttcggc agtctttcgt acacctacaa cattgcgcgc tcgttcgcgg
     2761 atatttcgtc ggtgcaggga caaacccagc cggcgacggt caagctcggc gacatcgtgc
     2821 aattcggcgg cggcgtggcg ctggcattct ccgacaagga ttcggcaagc atctcgtaca
     2881 cgatggcgct cgaacccgaa tcgaagacgc gtgcgccagg cggttcgtat gaaaaggtgc
     2941 cgggcagcga aaccacggcc gcggcgctga atttcggcct gaaccacgta gtgaacaagc
     3001 acctgaccat caacggctcg gtctccgtcg gcttgacgcc ggacgcgccg aacttcgtgg
     3061 tcggcgtgcg gtttccctac acattttgag tgttcccaaa cctgacgctt cgcgccaggc
     3121 cccccgagca ggcaagcaaa cttggggcgt tttattagac gtgtcaacga tacgaggtcg
     3181 aacgtgcgtg aatcacctca tctgaccagt gttgcttcgg tggcgggagc gggatcgcat
     3241 atgacgcgta cgctgcctgc ggaccgcccc gtcgaagccg taacgaaatg cgccgcggtg
     3301 gcggcggtgg tttcgatcgg tgcgccggct gcgggcgcga gcggtacgcc gtgcgcccat
     3361 gcgcgcgccg cacgcatgct gttgtacgtg gcgcgcacgc cggacgatac cctcgtcgcg
     3421 catctgcgaa gccgcggttg gaacgtcgcg gtggcgcgct cggcgcatga actcggcaga
     3481 ctcgtgaagc ccgacgcggc ctgcgccggc atcgtcgatc tggcgagctt tccgccgcgc
     3541 gatctgggtg gcctcgaagc cagtttgcgt cagcagcagg tcggctggat cgcgcttgcc
     3601 accaacgaaa gactcaacga tccgctggcg cgacgtctgg tacgccatta ctgcttcgat
     3661 tacgtgaaga ttccggtggc gaacgcgacg gtcgactatc tggtcagtca cgccttcggc
     3721 atggcgacgc tgggcgagcc ggaactcgct gcgccggtga atgagtccgg cgacgaggaa
     3781 atggtcggta cctgcgaggc gatgcagcag ctctttcgca ccatccgcaa ggtggcgaac
     3841 accgacgcga gcgtgttcat ctccggcgag tcaggcaccg gcaaggaact caccgcgctg
     3901 gcgattcacg aacgctcgcc gcgccgcaag acgccgttta tcgcgatcaa ttgcggggcg
     3961 attccgcatc atctgttgca atccgaacta ttcggttatg agcgcggcgc gttcaccggc
     4021 gcgaatcagc gcaagatcgg ccgggtcgaa gcggccgatg gcggcacgtt gctgctcgac
     4081 gaaatcggcg atctgccatt ggaaagccag gcgagcctgc tgcggtttct gcaggagggc
     4141 aagatcgagc gtctcggcgg ccgcgaatcg attccggtcg acgtgcggat catttcggct
     4201 acccacgtcg accttgaagg cgcgatgcgc gccgggcagt tccgtgccga cctgtttcac
     4261 cgcctgtgcg tgctgcgggt ggacgaacca ccgttgcggg cgcgcggcaa ggatatcgaa
     4321 atcctcgcgc accacgttct gcataagttc aagaccgata gcgcgcgcaa gatccgcggc
     4381 ttcacgccgt cggcgatcga agcgctgtat agctacagct ggcccggcaa tgtgcgcgag
     4441 ctgatcaacc gcgtgcgccg tgcgatcgtg atggcggaga gcaagctgat ttccgctgat
     4501 gacctggacc tcgcgcagtt caccgagcaa cagacgatga gcctttcgca ggcgcgcgac
     4561 gccgctgaga aacgcgcgat cgaagctgcg ctgctgcggc atcgtcaccg gctgaacgag
     4621 gcggcggcgg atctgaatat ttcgcgcgtg acgctgtacc ggctgatggg tcatcatgga
     4681 ctgcgggagc tgagtgcggc tgacgagaag gcgtcgcttg aatcgttcgc cgctgaggac
     4741 ccttcgcaaa agtgacggtg cggcctgcgt gtcagcaggt tgtggcgctg tggtacgccg
     4801 ccacggtgta tcgacgtggt gtatcgacgt ggtgtatcgc cgtggtgcgc cgccgtggtg
     4861 cgccgccaca gcggccgcac gggctggcgc ggccagcgag tgcatgaacg ctcgtcgcgc
     4921 tacagcgccg accgctccgg ttagcgcccg ttctgccgac cgtgcggttc gcgctcgcgc
     4981 ctcaggtaga atcatcccgg ttacgttccc ctttccattc gatgacgacg ctcaccctga
     5041 tcgtcgctcg cgccaataac ggcgtgatcg gccgcgacaa ccagttgccc tggcgacttc
     5101 ccgaagacct tgcgttcttc aagcgcacca cgatgggcgc gcccatcatc atgggccgca
     5161 agacgcatga atcgatcggc cggccgttgc cgggacgccg caacattgtc gtgacacggg
     5221 atgccacgcg gcgctttcag ggctgcgatg cggccaccac cctcgaggac gcgctggcgc
     5281 tcgccgccca ggaccaggcg ccggaggcgt ttctgatcgg cggcgcgcag ctgtatgcgg
     5341 aaggcctgcg tcaagcggac aagcttatca tcaccgagat ttccgccgac ttcgaaggcg
     5401 acgccacgtt ccccgaactc gatgaaaacg agtgggaaga agtcgcgcac gagacgcatc
     5461 gcgcggacgc gccgaatgac ttcgactatg cgttcgtgac ttataggcgc aagggtagtt
     5521 gaggttttgc gctgcgcctg aaggcgcggc ggtttgggct ttgcccttcg ggctttgggt
     5581 tcgggcttca tgcttctatc caaagcgtcg aacattgaca acgagcccac aacgcacgca
     5641 ctaagaaaaa acgccacgca gttgcttgcg tggcgtttct gtttgcggct tgttgcccgt
     5701 ttgccgcttg tcgccctctc cggcggctaa cggtctgcga gcagtccgc
//
LOCUS       DATASH010000321         1964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1035287,
            whole genome shotgun sequence.
ACCESSION   DATASH010000321 DATASH010000000
VERSION     DATASH010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035287"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..119)
                     /locus_tag="VHV99_09040"
     CDS             complement(<1..119)
                     /locus_tag="VHV99_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEX3635620.1"
                     /translation="MSTVNLAAYHFATIEDTAAWRPLVTERCNTLGLRGTVLLA"
     gene            608..>1964
                     /gene="dnaE"
                     /locus_tag="VHV99_09045"
     CDS             608..>1964
                     /gene="dnaE"
                     /locus_tag="VHV99_09045"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX3635621.1"
                     /translation="MSPMSDPRFVHLRVHSEFSIADGIVRLDDVVKAAAKDGQGALAL
                     TDLGNAFGLVRFYKEARGKGVKPIAGCDVWITNPDDREKPSRLLLLVKNRRGYLNLCE
                     LLSKASLTNQYRGRAEVEAGWLESGLGEGLLALSGAQQGDIGLALAAGNEEAAKRNAL
                     HWAKVFPGGFYIELQRCGQPGGEQYVQQAVALAASLKLPVVATHPMQFMTPDDFTAHE
                     ARVCISEGDILANPRRSKRFTSEQYFRTQEEMAELFADIPSALANSVEIAKRCNLTLE
                     LGKPKLPLFPTPDGMSLDDYLVQLSKEGLEKRLEQLYPVGAEREAQRETYYQRLEFEC
                     GTIIKMGFPGYFLIVADFINWAKNNGVPVGPGRGSGAGSLVAYALGVTDLDPLRYNLL
                     FERFLNPERVSMPDFDIDFCQEGRDRVIQYVKGKYGADAVSQIATFGTMAAKAAVRDI
                     GR"
BASE COUNT          371 a          612 c          619 g          362 t
ORIGIN      
        1 gccagcagga cggtgccgcg caggccgagc gtattgcagc gctcggtgac gagcgggcgc
       61 catgcggcgg tgtcttcgat agtcgcgaaa tgatacgcgg cgagattgac ggtactcatg
      121 tgtgtccagc agtaagaagt gcccggcggc gcggaaaacg gggcgcattg agacgcccgc
      181 attcagcctc cgtgcggccg gtcacgtcga ataggcgaaa cccgtattat ccctcaagcc
      241 ggcgcttagg ccgactcggc cgaatggcca gccagttgcg gcagcgcaaa tcgccagcaa
      301 acttgcacgg cgcttgcctc ggcgtactcc cacacgctgc tgaacgttcc tcattggcaa
      361 cgcgccgcac ggcgcagctt gaccgttcct cgcacgcccg caccggacgg tggattctgg
      421 ccagtgaaat atcgtcgaat gtgctccggc tggtccgtcc gggcgcgcaa ggcgtacgcc
      481 catgcgctgt caccgaaacc ttccactgcc cgcaaaccgc cgatgtacgg cgcgctcgcg
      541 tttccgtcct aagccgaacg gctaacgcgc ggttttggcc tgaaaactcc ggaagggtgg
      601 agttacaatg tcgcccatgt cagatccccg cttcgttcat ctccgcgttc actccgaatt
      661 ctcgattgcc gatggcatcg tgcgtcttga cgacgtcgtc aaggccgctg ccaaagatgg
      721 tcagggcgcg ctcgccctaa ccgacctcgg caacgcgttc ggcctcgtcc gtttctacaa
      781 ggaagcccgc ggcaaagggg tcaaacccat tgccggctgc gacgtctgga tcaccaatcc
      841 ggacgaccgc gagaagcctt cgcgtttgct gctgctggtc aagaatcggc gcggctatct
      901 gaatctgtgc gagctgctca gcaaggcatc gctcacgaat cagtatcgcg gccgcgcgga
      961 ggttgaagcc ggctggctcg aatcgggcct gggtgaaggt ctgctcgcgt tgtcgggtgc
     1021 gcaacagggc gacatcgggc tcgcgcttgc cgccggcaac gaagaagcgg ccaaacgcaa
     1081 tgccttgcat tgggctaagg tgttccccgg cggcttctat attgaactgc aacggtgcgg
     1141 tcagccgggc ggcgaacaat acgtgcagca ggcggtcgca ctggcggcgt cgctgaaatt
     1201 gccggtggtg gccacccatc ccatgcagtt catgacgccg gacgatttca ccgcgcatga
     1261 agcgcgcgtg tgtatttcag aaggcgacat cctggcgaat ccgcgccgct cgaagcgttt
     1321 tacgtcggaa cagtatttcc gcacgcaaga agagatggct gagttgttcg cggacattcc
     1381 gtcggcgctc gccaatagtg tcgaaatcgc caaacgctgc aacctcacgc tcgaactcgg
     1441 caagccgaag ctgccgctct tcccgacgcc cgacggcatg tcgctcgacg actacctcgt
     1501 gcaattgtcg aaagagggcc tggaaaagcg tctcgaacag ctgtatccgg ttggagccga
     1561 gcgcgaggcg cagcgcgaaa cgtattacca gcgcctcgaa ttcgagtgcg gcacgatcat
     1621 caagatgggc ttcccaggct acttcctgat cgttgcggac ttcatcaact gggcgaagaa
     1681 caacggcgtg ccggtcgggc cgggccgggg ttcgggcgca ggttcgctgg tcgcgtacgc
     1741 gctcggcgtg accgacctcg atccgctgcg ctacaacctg ctgttcgaac gtttcctgaa
     1801 tccggaacgg gtgtcgatgc ccgacttcga tatcgacttc tgccaggaag ggcgcgatcg
     1861 cgtcatccag tatgtgaagg gcaagtacgg cgccgacgcg gtgtcgcaga tcgcgacctt
     1921 cggcacgatg gccgcgaagg ccgcggtgcg cgatatcgga cgcg
//
LOCUS       DATASH010000322         1652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_837492,
            whole genome shotgun sequence.
ACCESSION   DATASH010000322 DATASH010000000
VERSION     DATASH010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837492"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1652)
                     /locus_tag="VHV99_09050"
     CDS             complement(<1..>1652)
                     /locus_tag="VHV99_09050"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MutS2/Smr-associated SH3 domain-containing
                     protein"
                     /protein_id="HEX3635622.1"
                     /translation="SGQTVYVEPLETIEQNNELVRLIEEEQAEIHRIFVALTRQVGGY
                     AQALVDGARVLALVDSLQARARFAREYDCVAPALAPDRLSLEGARHPLLEKRLRATRG
                     NIVPLVLELTAEERQLIISGPNTGGKTVALKTTALLAMMAQAGVPVPASAAIFPVFTA
                     FLADIGDAQSIEAALSTFSAHITNLDRLARLADARSLVLLDELGSATDPEEGAALAVA
                     VASFFLHAGAWTLISTHHTSLKVYAANTPGVRNAAAGVDETTLAPNYQLRQGVPGASA
                     GIQTAERLGLNRAIIAAARERLGSQQADIARFLDRLHNELTQLESERKATREQQYALN
                     QERARLAREGDVELRNRTRDLEAKLASLLKDFEFQMRENVRVIEDRAAQQKLSKEAER
                     RINRLRREFQESFNQTVVAHRTGADQGDANAQPHVLKHIAPGDQVRIKSMNKVAVVQR
                     EIEKDLFEVALGPIKMRVKRDELSEVLRTADSRTSAEANPLAAARRQKNVHISVTSAN
                     TDDMRMEINLIGRTVDEATEELEKYLDRAFLAGLPRVRVIHG"
BASE COUNT          274 a          525 c          527 g          326 t
ORIGIN      
        1 gaccgtgaat gacgcgcacg cgaggcagac cggcgaggaa ggcgcggtct agatactttt
       61 cgagttcttc cgtcgcctcg tccacggtgc ggccgatcaa gttaatctcc atgcgcatgt
      121 cgtctgtatt ggcgctggtg acggagatgt ggacgttctt ctgccggcgg gcggcggcga
      181 gagggttcgc ctctgcgctg gtccgagagt ctgccgtacg cagcacctcg gaaagctcgt
      241 cgcgcttgac gcgcatcttg atgggaccga gtgcgacctc gaagaggtct ttctcgatct
      301 cacgctgcac aactgcgacc ttgttcatcg atttgatccg cacctgatcg ccgggcgcga
      361 tgtgcttcaa cacgtgcggc tgcgcgttgg cgtccccctg atcggctccg gtgcgatgcg
      421 cgactacagt ctggttgaag ctctcctgaa actcgcggcg caggcgattg atacggcgtt
      481 cggcttcctt ggataatttc tgctgcgcgg cgcggtcctc gatgacgcgc acattctcgc
      541 gcatctgaaa ctcaaaatcc ttgagcagcg acgccagctt ggcttcaaga tcgcgcgtgc
      601 gattgcggag ctccacgtcg ccctcgcgcg ccagtctcgc ccgttcctga ttcagcgcgt
      661 actgctgctc gcgcgttgct ttgcgttcgc tctccagttg cgtcaactcg ttgtgcagac
      721 ggtcaaggaa gcgcgcaatg tccgcctgct gcgagcccag gcgctcgcgt gcggcggcaa
      781 tgattgcgcg attcaatccc agacgctcgg cggtctgaat acccgccgaa gcgccgggca
      841 cgccctggcg cagctgataa ttgggcgcga gcgtggtttc atccacgccc gcggccgcgt
      901 tgcgtacacc cggcgtgttg gccgcgtaga ctttgagcga agtatgatgc gtggagatca
      961 gcgtccacgc gcccgcatgg agaaagaagc tggccacagc cacggccagc gcggcgcctt
     1021 cttcggggtc agtagccgag cctagctcat cgagcagcac cagcgagcgc gcatcggcca
     1081 gccgcgccag ccggtcaaga ttggtgatgt gcgcggagaa ggtcgaaagc gccgcttcga
     1141 tggattgcgc gtcgccgatg tcggctaaga acgcggtgaa gaccggaaag attgccgcgc
     1201 ttgccggcac cggcacgccg gcctgcgcca tcatggccag cagtgcggta gtcttcagcg
     1261 caaccgtctt gccccctgtg ttcggcccgc tgatgatgag ctggcgttcc tcggcggtca
     1321 actccagaac caaaggaaca atgtttcctc tggttgcgcg cagccgcttc tcgagcaacg
     1381 ggtgacgcgc gccttcgagc gacagccgat ccggcgcgag agcgggcgcg acgcagtcgt
     1441 actcccgcgc aaaccgcgcg cgcgcctgca ggctgtcaac gagagccagc acgcgggcgc
     1501 cgtcgaccaa tgcctgcgcg tagccgccta cctgccgtgt gagagcgacg aagatgcgat
     1561 gaatctctgc ctgctcttct tcgatgagcc gcaccagttc gttgttttgc tcgatggttt
     1621 cgagcggttc aacgtagacc gtctgcccgg ac
//
LOCUS       DATASH010000323         2073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1233076,
            whole genome shotgun sequence.
ACCESSION   DATASH010000323 DATASH010000000
VERSION     DATASH010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1233076"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="VHV99_09055"
     CDS             complement(<1..837)
                     /locus_tag="VHV99_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3635623.1"
                     /translation="MSKLQLSVAVGPYDRVRPLVDADVQIEGVDPTFLLQEPEEIFFR
                     AFRHADYDICELSLSSYCVKTAAGTSPYIAVPVFPSRAFRHTSVYVNANAGINKPEDL
                     KGKRIGVPEYQLTANVWVRLFLEEEYGVKPSDVTWLRGGYEDPTRVEKITLNLPSDVK
                     LENAPEGKTISGMLEAGEIDALIGPRAPSCFDRGSANVKYLFDNPQAVAADWYRRTKL
                     FPIMHTLGIRRTLVEQHPWLPMSVMKAFEKSKAVALARLTDTSATKVTLPFIEDQLRA
                     ARK"
     gene            complement(855..1790)
                     /locus_tag="VHV99_09060"
     CDS             complement(855..1790)
                     /locus_tag="VHV99_09060"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016348001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDR/VanB family oxidoreductase"
                     /protein_id="HEX3635624.1"
                     /translation="MLLHVTGKIALADGIYQFEFRSPEGKPLPSFTAGAHVLIETPSG
                     IARRYSLCNAPSERDRYLVAVKLDPNSRGGSLSMINDVQIGMRMKVSVPENYFALTED
                     ASDYLLIAGGIGITPLRAMMAELDARSAQYRLVYCTRSPETTAFLAELSSLPSSHVQV
                     HHDHGNPEQSLDFAKLLATREGNTHLYCCGPRPLMQAVRDGASGWPSPAVHFEDFGTS
                     VSTGDASSGFTVTLQRSGIRVNVAPGQSILAALRESGVETPSSCEAGTCGACRTTLCS
                     GEADHRDFVLDDDEHDRAIMICVSRAKSDELVLDL"
     gene            complement(1839..>2073)
                     /locus_tag="VHV99_09065"
     CDS             complement(1839..>2073)
                     /locus_tag="VHV99_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3635625.1"
                     /translation="GLLVYTDEGMSSLDLRTDTSPRERVIAEFYDAITGRRPALHDAQ
                     WGLANLEICLAALHSSRNGCEVSLTRQCAPKSF"
BASE COUNT          406 a          608 c          645 g          414 t
ORIGIN      
        1 cttgcgggca gcgcgcaact ggtcttcgat gaacggcaac gtgaccttgg tagccgacgt
       61 gtcggtcagg cgcgcgagcg caaccgcttt cgacttctcg aatgccttca tcaccgacat
      121 cggcagccac gggtgctgtt caacgagcgt gcgccggatg ccgagcgtgt gcatgatcgg
      181 gaacagcttc gtgcgccgat accagtcggc ggcgactgcc tgcgggttgt cgaacagata
      241 cttcacgttc gcggaaccgc ggtcgaaaca cgacggtgca cgcgggccga tcaacgcatc
      301 gatttcaccg gcttcaagca tgccggaaat cgtcttgcct tcgggcgcgt tctccagctt
      361 tacgtcggag ggcaggttca gcgtgatctt ttcaacacgc gtcgggtctt cgtagccacc
      421 gcgcagccag gtcacgtcag acggcttgac gccgtactct tcttcaagaa agaggcgcac
      481 ccatacattc gcggtcagtt gatattcggg cacgccgatg cgtttgccct tcaagtcttc
      541 cggcttgttg atgccggcgt tcgcgttgac gtacacggac gtgtgacgga atgcgcgcga
      601 tgggaagacg ggcacggcga tatacggcga tgtgcccgct gcggtcttga cgcaatagct
      661 gctcagcgac agttcgcaga tgtcgtaatc ggcatggcgg aacgcacgaa agaagatttc
      721 ttcaggttcc tgcagcagga aagtcggatc gacgccttca atctgtacat ctgcgtcaac
      781 gagagggcgc acgcggtcgt aggggccgac cgccaccgaa agttgcaact tgctcacact
      841 atctccttca ggaattaaag atcgaggacc agctcgtccg acttcgcgcg cgaaacgcaa
      901 atcatgatcg ccctgtcgtg ttcgtcgtca tcgagcacaa aatcgcgatg atcggcctcg
      961 ccggaacaaa gtgtcgtccg gcaggctccg caggttccgg cctcacagga gctgggcgtt
     1021 tcaacgcctg actcacgcaa cgctgcgagg atgctttgtc ccggcgcgac attcacgcgg
     1081 atgccactgc gctgcagcgt gacagtaaag ccgctggatg catcgccagt cgacacgctg
     1141 gtgccgaaat cctcgaagtg caccgccggc gacggccagc ccgacgcacc gtcacggaca
     1201 gcctgcatga gcggacgggg gccgcagcaa tacaggtgcg tattgccttc acgagttgcg
     1261 agcagtttcg caaaatcgag tgactgttca gggttgccgt ggtcgtggtg aacctgaaca
     1321 tggctcgacg gcagcgatga aagttcggcg agaaaggcgg tcgtttccgg cgagcgcgtg
     1381 cagtacacga ggcgatattg ggcactgcgt gcatccagtt cggccatcat tgcgcgcagg
     1441 ggcgtgatgc caatgccgcc cgcgatcagc aggtagtcgg acgcatcctc ggtgagcgcg
     1501 aagtaattct ccggcaccga taccttcatg cgcatgccga tttgcacgtc attgatcatg
     1561 ctaagggagc cgccccggct gttcgggtcc agtttgaccg cgacaaggta acggtcgcgc
     1621 tcgcttggtg cattgcacag cgaatagcgg cgagcgatac ccgacggcgt ttcgatcagc
     1681 acgtgcgcgc cagccgtgaa cgacggtagc ggtttgcctt caggcgagcg aaattcgaat
     1741 tggtaaatgc cgtcggcaag cgcaatcttg ccggtaacgt ggagcagcat gttctcattc
     1801 atgtttcttc aaaccggtct tgccggcgtc tccctggatt aaaaggattt tggcgcgcat
     1861 tggcgcgtca gcgaaacttc gcagccgttg cgcgacgagt gcagtgctgc caggcagatt
     1921 tcgaggtttg ccagtcccca ttgcgcatcg tgcagcgcgg gccggcgccc ggtgatggcg
     1981 tcgtaaaact ccgcgatcac gcgctcgcgt ggcgacgtgt ccgtgcgcag atcgagtgag
     2041 gacatgccct cgtccgtgta gacgagcagt cca
//
LOCUS       DATASH010000324         3524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1035937,
            whole genome shotgun sequence.
ACCESSION   DATASH010000324 DATASH010000000
VERSION     DATASH010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035937"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /locus_tag="VHV99_09070"
     CDS             complement(<1..1038)
                     /locus_tag="VHV99_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013338602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX3635626.1"
                     /translation="MSVPLAQIPAPHDTASLTLVEQLVQWARRRIEERVFRPGMRMPS
                     IRKLALDKGVSRFTVVEAYERLVAQGYLESRRGSGFYVRERLGGAPTAEIRTIAAAAP
                     APATIDVVWLLRNMLHTGARPERSPGLGYLPGRWLDGDLITNALRTLGRQSGAQMLGI
                     GTPQGFLPLRQQLQTRLEELEIGASPDQIVMVSGITQAIDLISRIYVKSGDAVIVGDP
                     AWFQMFGRFASQGARLVGMPYTPDGPDLDALESLVQTWRPKMLVINSVLQNPTGTSLT
                     AAQAFRILRLAEAYDFIVVEDDIYGDLCPPSYPGTRLASLDQLKRVIYLGSFSKTLAP
                     NLRVGFIACAPE"
     gene            1317..2006
                     /locus_tag="VHV99_09075"
     CDS             1317..2006
                     /locus_tag="VHV99_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3635627.1"
                     /translation="MTAHSLRLDHLVISARTLDEGTQYVADTLGVAPAGGGAHPLMRT
                     HNRLLNLWGDVYLEVIAIDPQAAEPADGAAPRARLFALDDPATQARLEKGPCLSHWVA
                     RVERPKRLNVWQTQYPQRIPPIVPMTRGDFTWGLSVPDDGAFPAWQGAGDGVLPSLIQ
                     WDTPRHPSSVLPETGIALKALKAAHPQADTIAAQLHWLGAAHLIALEPTAGAAALVAE
                     FETPDGLRTLK"
     gene            2082..3278
                     /locus_tag="VHV99_09080"
     CDS             2082..3278
                     /locus_tag="VHV99_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006478175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX3635628.1"
                     /translation="MDQSDLKAPTWQLSERALKLTSSAIREILKVTERPEVISFAGGL
                     PSPATFPAERMREASDRILRDAPAAALQYSATEGYLALREWIAQRYSVNGAQIRPSQV
                     LITTGSQQALDLLGKVLVCPDSPVLVETPTYLGALQSFSMYEPRYVQVATDEQGLIPE
                     ALTPELTAGARLLYAQPNFQNPTGRRLPIERRRALAAFAKTAPFPVIEDDPYGALDYA
                     GEPLPTMLSMAPDHIVHLGSFSKVLAPGLRVGYIIAPEELIFKLVQAKQATDLHTPSF
                     TQRIVYEVIKDGFLDTHVPTIRELYRDQCAAMLASLERYMPEGVSWNRPEGGMFVWVN
                     LPAQIDSMKLLEEAVAQNVAFVPGAPFFANEAEHNTLRLSFVTVPPARIDEGVSRLAA
                     LIRAKV"
     gene            3333..>3524
                     /locus_tag="VHV99_09085"
     CDS             3333..>3524
                     /locus_tag="VHV99_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX3635629.1"
                     /translation="MAQTNVYDKLKELGIELPGAGAPAAAYVMSAQSGNTVYLSGHIA
                     KKDGNVWAGKLGATLTTEEG"
BASE COUNT          669 a         1202 c         1099 g          554 t
ORIGIN      
        1 ttccggcgcg caggcgataa aacccacgcg caaattcggc gcgagcgtct tcgaaaagct
       61 gcccaggtag atcacgcgct tcaactggtc gagactcgcg agccgcgtgc cgggatagct
      121 cggcgggcag agatcgccgt aaatatcgtc ttcgacgacg ataaaatcgt aggcctcggc
      181 gagacgcaga atgcggaacg cctgcgcggc cgtcagcgac gtgccggtgg ggttctgcag
      241 caccgaattg atcaccagca ttttcggccg ccaggtttgc accagcgact cgagcgcgtc
      301 gaggtccggc ccgtccggcg tgtagggcat gccgaccagg cgcgcgccct gcgacgcaaa
      361 gcgcccgaac atctgaaacc acgccggatc gccgacgatc accgcatcgc ccgacttcac
      421 atagatgcgc gaaatcaggt cgatcgcctg cgtaataccg gagaccatca cgatctgatc
      481 cggcgaagcg ccgatttcca gttcctccag ccgcgtctgc aactgctggc gcagcggcaa
      541 aaagccctgc ggcgtgccga tgccgagcat ctgtgcgccg ctttgccggc cgagcgtgcg
      601 cagtgcgttg gtgatcagat cgccgtcgag ccagcgcccc ggcaggtagc ccagacccgg
      661 gctgcgctcg gggcgcgcgc ccgtatgcag catattgcgc agcagccaga cgacgtcgat
      721 cgtggccggc gcgggcgccg ccgcggcgat cgtgcggatc tcagccgtcg gcgcaccgcc
      781 caatcgttcg cgcacataga aacctgaacc ccgccgcgac tccaggtagc cttgcgcgac
      841 cagccgctcg tacgcctcga ccacggtgaa gcgcgatacg cccttgtcca gcgccagctt
      901 gcggatcgac ggcatgcgca tgccgggacg gaagacgcgc tcctcgatgc ggcggcgcgc
      961 ccactggacg agctgctcga ccagcgtcag cgaggccgtg tcgtgcgggg cgggaatctg
     1021 ggcaagcggg acggacatga cgggctccaa ctgtaccgaa cggtatcgag tcgattgtac
     1081 cgttactgtg ccggtcgccg ccgttacatt gaaggctaca tttcagataa gcagcggatg
     1141 atgaagcggc ccgggcggta tgcaccgcac acggccaagc tcgggcaaga cccgcccaaa
     1201 ccccattcgt ctggcctgcc gacccggcaa acggttatgc ttacgacccc ggccacgctg
     1261 ctgccgcacc cggcgcgcgt tccggcttac cgcagaaacc gctttcccga tccgccatga
     1321 ctgcccattc gctccgtctc gatcatctcg tgatttccgc ccgtaccctc gacgagggca
     1381 cgcagtacgt cgccgacacg ctcggcgtcg caccggcagg cggcggcgcg catccgctga
     1441 tgcgcacgca caatcgcctg ctgaatctgt ggggtgacgt gtatctggaa gtgatcgcaa
     1501 tcgatccgca ggccgccgaa cccgcggatg gcgccgcgcc ccgcgctcgc ctgttcgccc
     1561 tggacgaccc ggccacccaa gcacggctcg aaaaagggcc ctgcctgtcg cactgggttg
     1621 cccgagtcga gcggccgaag cggctcaatg tgtggcaaac gcagtatccg cagcgcatcc
     1681 caccgatcgt gccgatgacg cgcggcgatt tcacgtgggg cctgtcagtg cctgacgacg
     1741 gcgccttccc tgcctggcaa ggcgcgggcg acggcgtgct gccgtcgctg atccagtggg
     1801 acacgccgcg ccatccgtcg agtgtcttgc cggaaacggg cattgcgctg aaagcattga
     1861 aagccgcgca tccgcaagcc gacacgatcg cggcgcaatt gcactggctg ggcgccgcgc
     1921 acctgattgc actcgaaccc acggcaggcg cagcggcgct cgtcgccgag tttgaaacac
     1981 ccgacggtct gcggaccctt aaataagcag cgcgcgcggg tcgccgactc gcgcgataat
     2041 tgaacttttg accggttcca gagataccag cagaggagac catggaccaa agcgacttga
     2101 aagcccccac gtggcaactg tccgaacgcg cactcaagct cacgagctcg gcgatccgcg
     2161 agatcctgaa ggtcacggaa cggcccgaag tcatttcgtt cgcgggcggt ctgccctcgc
     2221 cggcgacctt cccggccgaa cgcatgcgcg aagcgtccga ccgcattctg cgcgacgcac
     2281 ccgccgcggc tttgcaatac agcgcgaccg aaggctacct ggcgctgcgc gaatggatcg
     2341 cacaacgcta ttcggtgaac ggcgcgcaga ttcgcccgtc gcaggtattg atcaccactg
     2401 gctcgcagca agcgctcgac ctgctcggca aagtgctggt gtgcccggac agccccgtgc
     2461 tggtcgaaac gccgacctac ctcggcgcat tgcaatcgtt ctcgatgtac gaaccgcgct
     2521 atgtgcaagt ggcgaccgac gagcaaggcc tgattcccga ggcgctcacg cccgaactga
     2581 cggccggcgc acgcctgttg tacgcacagc cgaatttcca gaatccgacg gggcgccgcc
     2641 tgccgatcga acgccgccgc gcactcgccg cgttcgcgaa gaccgcgccg ttcccggtaa
     2701 tcgaagacga tccctacggc gcgctcgact acgcaggtga accgctgccc accatgttgt
     2761 cgatggcgcc ggatcacatc gtccacctcg gctcgttctc gaaagtgctg gcgccgggcc
     2821 tgcgggtcgg ctacatcatt gcgcccgagg agctgatctt caagctcgtg caagccaagc
     2881 aagccaccga tctgcacacg ccgagcttca cgcaacgcat cgtgtacgag gtgatcaagg
     2941 acggcttcct cgacacccac gtgccgacca ttcgcgagct gtaccgcgac caatgcgcgg
     3001 cgatgctcgc ctcgctcgaa cgctacatgc ctgaaggcgt aagctggaac cgtccggaag
     3061 gcggcatgtt cgtgtgggtg aatctgcccg cgcaaatcga cagcatgaag ctgctcgaag
     3121 aagccgtcgc gcagaatgtc gcgttcgtgc cgggcgcccc gttcttcgcg aacgaagccg
     3181 agcacaatac gctgcgcctg tcgttcgtca cggtgccgcc ggccaggatc gacgaaggcg
     3241 tatcgcgtct cgcggcgctg atccgcgcga aggtctaagc gacccccggc gcctcgccgg
     3301 gttaccgaat tcccttactg aaaggacaca ccatggctca aacgaatgtg tacgacaagc
     3361 tcaaggaatt gggcatcgaa ctgccgggcg caggcgcgcc ggcagctgcg tatgtgatga
     3421 gcgcgcaaag cggcaacacg gtgtacctgt cgggtcacat cgcgaagaag gacggcaacg
     3481 tgtgggcggg caagctaggc gccacgctca ccaccgaaga aggc
//
LOCUS       DATASH010000325         3059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2024483,
            whole genome shotgun sequence.
ACCESSION   DATASH010000325 DATASH010000000
VERSION     DATASH010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2024483"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..443)
                     /locus_tag="VHV99_09090"
     CDS             complement(18..443)
                     /locus_tag="VHV99_09090"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007747477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX3635630.1"
                     /translation="MKRKGSAVWQGGLQDGKGSISTDSGVLKDTQYSFATRFADGIGT
                     NPEELIAAAHAGCFSMALSAELGKAGITPERIATTAAVTLDKDGGGFAITAVHLDVAV
                     KIPGGDKAAFEKATADAKANCPVSKVLNATITMDAKLES"
     gene            complement(715..1269)
                     /locus_tag="VHV99_09095"
     CDS             complement(715..1269)
                     /locus_tag="VHV99_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006046909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX3635631.1"
                     /translation="MPQRLPHFELSDATPEQKTVLDEILAGPRGNLNGPFLGWIHSPE
                     LAQQAQRLGAFCRYRTGLPLRLSELAILLTAARWRAQAEWHIHYPIALQAGVAEADAE
                     AIRQGRRPVFADADDALIHDFASELYDTKRVSDVTYTKAVERFGHQVVINLVGLLGYY
                     ALVAMTLNVFDMRAVGQESQPFAE"
     gene            complement(1298..1696)
                     /locus_tag="VHV99_09100"
                     /pseudo
     CDS             complement(1298..1696)
                     /locus_tag="VHV99_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492129.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(1720..2574)
                     /locus_tag="VHV99_09105"
     CDS             complement(1720..2574)
                     /locus_tag="VHV99_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018438220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEX3635632.1"
                     /translation="MPDSFDQLAALIRARFSELSPQFQMGAGFLLDHPDEVAVSSMRK
                     VAERAQVQPASLVRLSQQLGFPGWNELRNLFVARVRTRPEPLTSRARSLVKSKDALAN
                     DLLVAQQHNLEVTAAHNGRVIVETARLLRRSPHVHVAGFRSCYAVAFGLVYGYRLFRS
                     SVSLLNGEAGTLEMQLRTVERDSATIVISFAPYSVEAARVAEAALEKGSKLIAITDSA
                     VSPIALNADKVLIFSHESPSFFPSLVAATAISEALVAHLLALEGAGAVEQLGIAEQSL
                     HDKGAYVP"
BASE COUNT          615 a          989 c          903 g          552 t
ORIGIN      
        1 gtggatgaac ggctcgctca gctttcgagc ttcgcatcca tcgtgatggt ggcgttcagc
       61 accttggaaa ccggacagtt cgccttggcg tccgcggtgg ccttttcgaa cgcggccttg
      121 tcgccaccgg ggatttttac ggccacatcg agatgcaccg cggtgatcgc gaaaccgccg
      181 ccgtccttat cgagcgtgac ggctgccgtg gtggcgatgc gttccggcgt gatgcccgcc
      241 ttgcccagtt cggccgacag cgccatcgag aaacaacccg cgtgcgctgc cgcaatcagt
      301 tcttccggat tcgtgccgat gccgtccgcg aagcgtgtcg caaacgaata ctgcgtgtcc
      361 ttgaggacgc cgctgtcggt ggaaatcgag cccttgccgt cttgcaggcc gccttgccag
      421 actgctgatc ccttgcgctt catcgctctc tccttgtgtg ccctgcttcg cgccgccgca
      481 caaccgccgc acaacctggt gacgccgccc gcaatggtcc agcccgggta ttcgcggtat
      541 cggcatcggt tgtgcgtccc ctcaggggac ttccttcagg acgtatcgcg cggcgctgca
      601 ggcgcgggtt ggaccgaacc tcatcatagg cgcttccgtg tgccagtgtc gtgacgatgc
      661 cgggcgtctt tgcaaaagac ccccggcacg accggaaagc accagcgcgg cctgctactc
      721 ggcgaacggc tggctctcct gtcccaccgc ccgcatgtca aacacgttca gcgtcatcgc
      781 gaccagcgcg tagtagccga gcaggcccac cagattgatc accacctgat gaccgaagcg
      841 ctcaaccgcc tttgtatagg tcacatccga cacgcgcttc gtgtcataca gttcgctcgc
      901 gaagtcatgg atcaacgcat catcggcgtc cgcgaagacg gggcgacgcc cctggcggat
      961 cgcctcggcg tcagcttcgg ccaccccggc ttgcaacgcg atcgggtaat ggatgtgcca
     1021 ctcggcctgt gcccgccaac gcgctgcggt tagcagaatc gcgagttccg acaggcgcag
     1081 cggcaagccc gtgcgatagc ggcaaaacgc gccgagacgc tgcgcctgct gcgccaactc
     1141 cgggctatga atccagccga gaaacggccc gttcagattg ccgcgcgggc cggccaggat
     1201 ctcgtcgagc acggtcttct gttcgggcgt ggcatcggac agttcgaagt gaggcaaacg
     1261 ctggggcatc gcaaatctcg caggaatgga aagggtctca gacggcggcc agcgcgcctt
     1321 cgatcgcatc ggcaaggcgg ctgacgatct cgtcgatgtc gcgcgcggtg caaatgaacg
     1381 gcggcgccag cagcacatga tcgccgatct tgccgtcgac cgtgccgccc atcggataca
     1441 ccatcaagcc gcgcgcgaac gcttcgcgcc ggatcgccgc gtgcagtttc agcttcgcgt
     1501 cgaatggcgc cctggtggcg cggtcccgca ccagttcaag gccgacaaag aggcctcgcc
     1561 cgcgcacgtc gccgatatgc ggatgctgcg catagtgctc gcgcagccgg ccacggagtt
     1621 gctcgccgcg cgcctgcacg ttctgcagca gatgctcgtc ggcaatcacg cgttgcactt
     1681 cgagcgccgc ggcgcaaaaa acggctcaga cgacaaacat caaggcacgt acgcgccctt
     1741 gtcgtgcagc gattgctcgg cgatgccgag ttgttcgacg gcacccgccc cttccagcgc
     1801 gagcagatgc gcaaccagcg cttcggaaat cgccgtggcc gccaccagcg aaggaaaaaa
     1861 cgacgggctt tcgtgcgaaa agatcagcac cttgtcggca ttcagcgcga tcggcgagac
     1921 cgcgctgtcc gtgatcgcga tcagcttgct gcctttttcc agagcggctt cggccacgcg
     1981 cgccgcttcg accgaatacg gcgcgaaact gatgacgata gtcgcgctgt cacgctcgac
     2041 cgtgcgcagt tgcatctcca gcgtgccagc ctcgccgttg agcaacgaca ccgacgagcg
     2101 aaacagccgg tagccataga caagaccgaa cgccaccgca taacaggagc gaaagcctgc
     2161 tacatgtaca tgcggcgagc gccgcaacag gcgcgccgtt tccacgatca cgcgaccgtt
     2221 atgcgcggca gtgacttcga gattgtgttg ttgcgcgacc agcaggtcgt tggccagcgc
     2281 atcttttgat ttgacgaggg aacgggcgcg gctggtgagc ggctcgggcc gcgtacgcac
     2341 gcgcgcaacg aacagattgc gcaattcgtt ccagccgggg aagcccaatt gctgcgacag
     2401 acgcaccagc gaggccggtt gcacctgtgc ccgttcggct actttgcgca tggatgagac
     2461 cgcgacttcg tcgggatgat cgagcaggaa ccctgctccc atctggaact gcggactgag
     2521 ttcggagaag cgcgcccgga tcagggcggc aagctgatcg aaactgtctg gcatgcgata
     2581 taccgggcga acggtcgatt aggatgacaa caaatgttac caccccgtcc cgactgagct
     2641 tgccgaagac aagtctgtgg tgagtggcaa acgaaatctg atttgtcgca aacaaacgtg
     2701 tcctgaccat tcagcgcaaa taaaactgtt ctgcaaatcg acctcagcgt tcgactccct
     2761 tatgcctaaa gggattgccg gcagttcccc tactcggcaa acacccattt tcccgcgttg
     2821 taaaaactgc caacgcgcca cttgaatgag gtccaacaac ggtgcgcaat ccccgcccct
     2881 taacaatcaa ccacttagca aaaaggcttg caaataaaag tggcccgaga aatagctctc
     2941 gcaaacgaaa tcttgcctgc caaaaacaaa cccatcttat tgatttataa ggacgttccc
     3001 gcgaatcacg ctgatcgcca gatggcaaac cgaaagggcg actcgcgtag cggtccggc
//
LOCUS       DATASH010000326         7418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2618634,
            whole genome shotgun sequence.
ACCESSION   DATASH010000326 DATASH010000000
VERSION     DATASH010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7418
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2618634"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(206..1861)
                     /gene="aceF"
                     /locus_tag="VHV99_09110"
     CDS             complement(206..1861)
                     /gene="aceF"
                     /locus_tag="VHV99_09110"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEX3635633.1"
                     /translation="MSQAIEVKVPDIGDYKDIPVIEVLVKAGDTVEKEQSLVTLESDK
                     ATMDVPSSAAGVVKEVKVKVGDNVSEGSLIVVLEGAEGGAAAPAPAPAAAPAPAPAPA
                     AAPAAAPAPAAAGGGGLQEVKVPDIGDYKDIPVIEVAVKVGDRVEKEQSLVTLESDKA
                     TMDVPSSAAGVVKEVKVKVGDNVSEGSVIVVLEADGGAAAPAPAARQEVAEKPSDAPA
                     APSPAPASPSALAQAPLIPAGEGGARHASHASPSVRKFARELGVDVTQVQGTGPKGRI
                     TQADVTAFIKGVMTGQRAAPAGAAAPAAGGGELNLLPWPKVDFTKFGPVDPKPLSRIK
                     KISGANLHRNWVMIPHVTNNDEADITELEALRVQLNKENEKAGVKITMLAFVIKAVVS
                     ALKQFPTFNASLDGDNLVFKQYFHIGFAADTPNGLVVPVIRDADKKGLIDIAKEMTEL
                     SKAARDGKLKPDQMQGGCFSISSLGGIGGTNFTPIINAPEVAILGLSRGAMKPVWDGK
                     QFVPRLILPLSLSYDHRVIDGAAAARFNAYLGALLADFRRVIL"
     gene            complement(1946..4642)
                     /gene="aceE"
                     /locus_tag="VHV99_09115"
     CDS             complement(1946..4642)
                     /gene="aceE"
                     /locus_tag="VHV99_09115"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006478378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEX3635634.1"
                     /translation="MSAVPDEVMKYVAAEKDDDPQETGEWLEALDGVISAVGPDRAHY
                     LIEKQIEFARVHGEHLPFSANTPYINTIPVSRQAKIPGDQDLEHRIRSYTRWNAIAMV
                     LRAGKDTNVGGHIASFASAATLYDVGYNHFWHAASAEHGGDLVFVQGHSSPGVYSRAF
                     LLGRLTENQLDNFRQEVGGEGISSYPHPWLMPDFWQFPTVSMGLGPIMAIYQARFMKY
                     MQARGIAKTEGRKVWAFLGDGETDEPESLGAIGMAGRERLDNLVFVINCNLQRLDGPV
                     RGNGKIIQELESEFRGAGWNVIKVIWGSRWDALFQRDKSGALMRRMMDVVDGEYQTYK
                     SESGAFVREHFFNTPELKALVAEWSDEDIWNLNRGGHDPHKIYAAFQEASNSQGQPTV
                     ILAKTIKGYGTGEAGQAMNITHQQKKLQVDQLKKFRDQFRLPISDEDIVDVPYLKFEE
                     GSKELEYMRARRQDLGGYLPARRQKAESLPVPALSAFEPLLKGTGEGREISTTMAFVR
                     ILNILLKDKALGKRIVPIVPDESRTFGMEGLFRQIGIWNQDGQKYVPEDSDQLMFYRE
                     SETGQILQEGINEAGGMADWIAAATSYSTHGEIMIPFYIFYSMFGFQRIGDLAWAAGD
                     MRSRGFLLGGTAGRTTLNGEGLQHEDGHSLLWAASVPNCISYDPTFGYELAVIMQDGL
                     RRMVADQEDVYYYITVMNENYEHPAIPQGDAVAADIIKGMYSFRKAEADKKAPRVQLM
                     GAGTIFNEVIAAADLLKNDWGVAADLWSVPSFTELAREGHEVQRWNLLHPTEEKKVSH
                     VEKLLKDAQGPVIASTDYVRALTEQIRAFVPQKFVVLGTDGYGRSDTREKLRHFFEVD
                     RYWVTVAALNALADEGTIERKVVAEALKKYDLDPAKPNPMTV"
     gene            4917..>7418
                     /locus_tag="VHV99_09120"
     CDS             4917..>7418
                     /locus_tag="VHV99_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX3635635.1"
                     /translation="MLTERLFARSARTPGSPGDSTPSSRWHHGPWWSNSYLLTPLLSI
                     LVFLVVMSLILWSLNRREQQQQEDTLYRNVAWAQQQIRLSMTGAQEQIQALARDLVAG
                     HADPHSFQVSTTDIMQGHPEILYMNWYTSQQQPHWPNTALPVFGQRLAKPNDAQMDEA
                     VKAAFNEARTTRRQVYSPLIYDDLGNGYITLQTPVYRDREFLGTVAAVFSVEGILKHD
                     IPPELSAKYKISIIDVNNRELTTTSTRPRLPRDMFYDLPLDPPGQGVSVRVYAFPQMT
                     NFTNNTLVWLVAGLSCFVLWSLWSLWKHTRQRFEAQQALYAEAFFRRAMENSVLIGMR
                     VLDMHGRITHVNPAFCRMTGWDESDLVGKNAPFAYWPRDAYPEMQRQLDMTLRGKAPS
                     SGFELRVRRKDGSLFHARLYVSPLIDSSGRQTGWMSSMTDITEPKRAREELAAAHERF
                     TTVLESLDAAVSVLAADEAELLFANRYYRHLFGIRPDGHLELAGGGFDSAQASSDSID
                     MVDTYAGLPAAALTESTADAQEIYVQSIQKWFEVRRQYIQWVDGHLAQMQIATDITTR
                     KQAQELSRQQDEKLQFTSRLMTMGEMASSLAHELNQPLAAINNYCSGTVALVKSGRTT
                     PDNLLPVLEKTAQQAVRAGMIIKRIREFVKRSEPKRQATRVADIVADAVGLAELEARK
                     RKIRIVTDLRSRLPVIYVDPVLIEQVLVNLLKNGAEAMHDARPNAVDPVIRVVVRLEG
                     GFVCISVVDQGPGVDEATAERLFEPFYSTKSDGMGMGLNICRSIIESHRGRLWVVNNV
                     ESDGHITGATFHCSLPIGEPDGPSNGGSEAPTPQTV"
BASE COUNT         1400 a         2405 c         2199 g         1414 t
ORIGIN      
        1 aagctggcag cgcggcgaac agaaacatcc tttgccgtcc gcccaaggac gcgcctttct
       61 tttgcctact tttctttgga agacaaagaa aagtaggtgc cgccccgcac aggggcgacg
      121 ctaatagacc gacaagaaag caggttctac agaagcgcgc ggataacaga caaaaaaccg
      181 cgccccgcgc aacccaatca ccacatcaaa gaatcacacg ccggaaatcg gcaagaagcg
      241 ccccaagata cgcattgaat cgcgccgcag cggcaccatc aatcacccga tggtcatacg
      301 acaacgacag cggcagaatc aaccgcggca caaactgctt gccatcccaa accggcttca
      361 tcgcgccgcg cgacaaccca agaatagcga cttcaggcgc attgatgatc ggcgtgaagt
      421 tcgtcccacc aatgccaccc aaggaagaaa tcgagaagca cccaccttgc atctgatccg
      481 gcttgagctt gccatcgcgc gccgccttcg aaagctcagt catttccttc gcgatatcga
      541 tcaaaccctt cttgtccgca tcgcgaataa ccggcacgac cagaccattc ggcgtatcgg
      601 cagcaaaccc aatatggaaa tactgcttga acaccaggtt gtcgccgtcg aggctggcat
      661 tgaacgtcgg gaactgcttc aaggcggaca caaccgcctt gatcacgaac gccagcatcg
      721 tgatcttcac gccggccttt tcgttttcct tgttcaactg cacgcgcagc gcttcgagct
      781 cggtgatatc cgcttcgtcg ttgttcgtga cgtgggggat catcacccag ttgcgatgca
      841 ggttcgcgcc cgaaatcttc ttgatgcgcg acagcggctt cggatcgacc ggaccgaatt
      901 tggtgaagtc gaccttcggc cacggcagca ggttcagctc accgccaccg gcagcaggcg
      961 cggcagcacc ggccggcgca gcacgctgac cggtcatcac gcccttgatg aaagccgtga
     1021 cgtctgcttg cgtaatgcgg cccttcgggc ccgtgccctg cacctgcgtc acgtcgacac
     1081 cgagttcgcg cgcgaacttg cgcacggacg gcgaagcatg actcgcatga cgcgcgccgc
     1141 cttcgccagc cggaatcagc ggcgcctgcg cgagcgcgga cggcgaagcc ggagcgggcg
     1201 acggagccgc cggcgcatcc gacggcttct cggccacttc ctgccgggct gcgggcgccg
     1261 gcgcagcagc accgccatcc gcttccagca cgacgatcac cgagccttcc gacacgttat
     1321 cgccgacctt gaccttcact tccttgacga caccggcggc cgagctcggc acgtccatgg
     1381 tcgccttgtc ggattcgagc gtcaccagcg actgctcttt ctcgacgcga tcgccgacct
     1441 tcaccgcgac ttcgatcacg ggaatgtctt tgtagtcgcc gatatccggc actttgactt
     1501 cttgcagacc accaccaccg gcggcagcag gcgccggcgc ggcagcgggt gcggcggccg
     1561 gtgcaggtgc cggagccgga gcagcagccg gtgccggagc aggcgcggca gcaccgcctt
     1621 cagcgccttc cagcacgacg atcagcgagc cttccgacac gttgtcgccc accttcacct
     1681 tcacttcctt gacgacgccg gcggccgagc tcggcacgtc catggtcgcc ttgtcggatt
     1741 ccagcgtaac gagcgattgc tctttctcga cggtatcacc cgccttcacc agcacctcga
     1801 tcacagggat gtccttgtaa tcgccgatgt ccggcacctt gacttcgatc gcttgactca
     1861 ttgttagtgt ctcctgggcc gcgcacgccg cctgccctgt tgatcagagc aggcgcgcac
     1921 gccgtggcac acggggaatg atgccttaga cggtcatcgg gttgggtttg gcgggatcaa
     1981 ggtcgtactt cttgagcgcc tcggcgacga ccttgcgttc gatcgtgcct tcgtctgcca
     2041 atgcattcaa cgcggcaacc gtgacccagt agcggtcgac ttcgaagaag tgacgcagct
     2101 tttcgcgcgt atccgaacgg ccgtagccat ccgtgcccag cacgacgaac ttctgcggca
     2161 cgaacgcgcg gatctgttcg gtcagcgcgc gcacgtagtc ggtcgatgcg atgaccggac
     2221 cttgcgcgtc cttcaggagc ttctcgacgt gcgagacttt cttctcttcg gtcgggtgca
     2281 gcaggttcca gcgttgcact tcgtggcctt cgcgcgccag ttcggtgaag ctcggtacgc
     2341 tccacaggtc ggctgcgacg ccccagtcgt tcttcagaag atcagcggcg gcgatcactt
     2401 cattgaagat cgtgcccgcg cccatcagct gaacgcgcgg cgctttcttg tcagcttcag
     2461 ccttgcggaa ggaatacata cccttgatga tgtcggccgc tacagcatcg ccctgcggaa
     2521 tcgccgggtg ctcgtagttt tcgttcatca ccgtgatgta gtagtacaca tcttcctgat
     2581 ccgcgaccat gcggcgcaga ccatcctgca tgatcaccgc gagttcgtaa ccgaaggtcg
     2641 ggtcgtagct gatgcagttc ggcaccgaag ccgcccacag cagcgagtgg ccgtcttcgt
     2701 gctgcagacc ttcgccgttc agcgtcgtgc ggccagcggt gccgcccagc aggaagccgc
     2761 gcgagcgcat atcgcccgcg gcccacgcca gatcgccgat acgctggaag ccgaacatcg
     2821 aatagaagat gtagaacggg atcatgatct cgccgtgcgt cgagtacgac gtcgcggcgg
     2881 cgatccagtc agccataccg ccggcttcgt tgatgccttc ctgcaggatc tgaccggttt
     2941 ccgactcacg gtagaacatc agctggtcgg aatcttccgg cacgtacttc tggccgtcct
     3001 gattccagat accgatctga cggaacaggc cttccatacc gaaggtacgc gactcgtccg
     3061 gcacgatcgg cacgatgcgc ttgccgagcg ccttgtcctt cagcaggatg ttcaggatcc
     3121 gcacgaacgc catcgtcgtg gaaatctcgc ggccctcgcc cgtgcccttg agcagcggct
     3181 cgaatgccga cagcgccggc accggcagcg actcagcctt ctgacgacgc gccggcagat
     3241 agccgccgag gtcctgacgg cgagcgcgca tgtactcgag ttctttcgaa ccttcttcga
     3301 acttgaggta cggcacgtcg acgatatctt cgtcggagat cggcagacgg aactggtcgc
     3361 ggaatttctt cagctgatcc acttgcagct tcttctgctg gtgggtgatg ttcatcgcct
     3421 ggccggcttc gcccgtgccg tagcccttga tcgtcttcgc gagaatgacg gtcggctggc
     3481 cctgcgaatt cgacgcttcc tggaacgctg cgtagatctt gtgcggatcg tggccgccgc
     3541 ggttcaggtt ccagatgtcc tcgtcggacc attcggcgac cagcgccttc agttccggcg
     3601 tgttgaagaa gtgttcgcga acaaacgcgc ccgactccga cttgtacgtc tggtattcgc
     3661 cgtcgacgac gtccatcatg cggcgcatca gcgcgcccga cttgtcgcgt tggaacagcg
     3721 catcccagcg gctgccccag atgaccttga tcacgttcca gccggcgccg cggaattcgc
     3781 tctccagttc ctggatgatc ttgccgttac cgcgcaccgg accgtcgagg cgctgcaggt
     3841 tgcagttgat cacgaacacg aggttgtcga ggcgttcgcg gccggccatg ccgatcgcgc
     3901 cgagcgattc cggctcgtcc gtttcgccgt cgccgaggaa ggcccagacc ttgcggcctt
     3961 cggtcttcgc aatgccgcgc gcctgcatgt acttcatgaa gcgcgcctga tagatcgcca
     4021 tgatcgggcc gagacccatc gagacggtcg ggaattgcca gaagtccggc atcagccacg
     4081 gatgcgggta cgacgagatg ccctcgccgc ccacttcctg acggaagttg tcgagctggt
     4141 tctcggtcag acggccgagc aggaacgcgc gcgagtagac acccggcgac gaatggccct
     4201 gcacgaacac gaggtcgccg ccatgttcgg ccgacgccgc atgccagaaa tggttatagc
     4261 cgacgtcgta cagcgtggcg gcggaagcga acgaggcgat atggccgccc acgttggtgt
     4321 ccttgccggc gcgcagcacc attgcaatgg cgttccagcg cgtgtacgag cggatgcggt
     4381 gttcgaggtc ctggtcgccg gggatcttcg cctgacgcga gaccgggatc gtgttgatgt
     4441 acggcgtatt ggcggagaac ggcaaatgtt cgccgtgtac acgggcgaat tcgatctgct
     4501 tctcgatcag gtaatgggcg cgatcggggc ccactgcaga aatcacgcca tccagcgctt
     4561 ccagccattc gccggtttcc tggggatcgt cgtctttttc ggcggcgaca tatttcatga
     4621 cttcgtcggg tacagcggac atgctcgtct cctggatata gaggaacgct cttgaacaga
     4681 cggcgcggac gaagcacgac cgcggcagct tcggccgatt gtaaaagggg tcccgacgtc
     4741 cgcgcaacaa aattttcgaa ttacgggatt gattctcata atgcggaaaa ttgttgcggc
     4801 gcaacttgat ttgactgccg atcgacggat acgtcggcta gtttgcgccg aataacggtt
     4861 ctttcagcgc ttttttgcgc catttgctag cgacttgctg cgctacaatg ccacgcatgt
     4921 tgaccgaacg gcttttcgct cgctcggcgc gtacccccgg ttcgccgggc gattcgacgc
     4981 cgtcctcccg ctggcaccac ggaccgtggt ggtcgaactc ctatttgctc acgccgctcc
     5041 tgtcgatcct ggttttcctg gtcgtcatga gcctgattct gtggagcctg aaccggcgcg
     5101 aacagcagca gcaggaagac acgctctacc gcaatgtcgc gtgggcgcag cagcagatcc
     5161 gcctgtccat gacgggcgcg caggaacaga tccaggcgct cgcccgcgat ctggtcgccg
     5221 gccacgccga tccgcattca ttccaggtgt ccaccacgga catcatgcag gggcatcccg
     5281 agatcctcta catgaactgg tacaccagcc agcagcagcc gcactggccc aataccgcac
     5341 tccccgtgtt cggccagcgt ctcgccaaac cgaacgacgc gcagatggac gaagccgtca
     5401 aagccgcctt caacgaggcg cgcaccacgc gccgccaggt ctactcgccg ctgatctacg
     5461 acgacctcgg caacggctac atcacgctgc agacgccggt ctatcgcgac cgcgaattcc
     5521 tcggcacggt tgcagccgtg ttctcggtgg aaggcatcct gaagcacgac atcccgcccg
     5581 agctgtcggc caaatacaag atttcgatca tcgacgtgaa caaccgcgag ttgaccacca
     5641 cgtcgacccg cccgcgcctg ccgcgcgaca tgttctacga tctgccgctc gacccgccgg
     5701 gccagggcgt ctcggtgcgc gtctatgcgt tcccgcagat gaccaacttc accaacaaca
     5761 cgctggtctg gctggtggcc ggcctgtcgt gcttcgtgct gtggagtctg tggagcttgt
     5821 ggaaacacac gcggcagcgc tttgaagcgc agcaggcgct ttatgctgaa gccttcttcc
     5881 gccgcgcaat ggagaactcg gtgctgatcg gcatgcgggt gctcgacatg catggccgca
     5941 ttactcacgt caatcctgcg ttttgccgta tgaccggctg ggacgaaagc gatctggtcg
     6001 gcaagaacgc gccgtttgcg tactggccgc gcgacgccta ccccgagatg caacgccagc
     6061 tggacatgac gctgcgcggc aaggcgcctt cttcgggctt cgaattgcgg gtgcgccgca
     6121 aggacggttc gttgttccat gcgcgtctct atgtctcgcc gctgatcgac agctcggggc
     6181 gccagaccgg ctggatgtcg tcgatgaccg acatcaccga gccgaaacgc gcgcgcgaag
     6241 aactcgccgc cgcgcacgag cgcttcacga ccgtgctgga aagtctcgac gccgcggtct
     6301 ccgtactggc cgccgacgaa gccgaactgc tgttcgccaa ccgctattac cgccacctgt
     6361 tcggcattcg cccggacggt catctggaat tggcgggcgg cgggttcgat agcgcacagg
     6421 cgtcatccga ttcgatcgat atggtggaca catacgcggg tctgcccgcc gccgcgttga
     6481 ccgaaagcac cgccgatgcg caggaaatct acgtgcaaag catccagaag tggttcgaag
     6541 tgcgccgaca gtacatccag tgggtggacg gccacctcgc gcagatgcag atcgcgaccg
     6601 acatcaccac gcgcaagcag gctcaggagc tctcgcgtca gcaggacgag aaactgcagt
     6661 tcaccagccg cctgatgacg atgggcgaaa tggcctcctc gctcgcgcac gaactgaacc
     6721 agccgctcgc cgcgatcaat aactattgct cgggaaccgt ggcgctggtt aaatccggtc
     6781 ggacgacgcc cgataatctc ctgcccgtgc tcgaaaaaac cgcccagcag gcggtgcgcg
     6841 caggcatgat catcaagcgc attcgcgagt tcgtgaaacg cagcgagccg aaacgccagg
     6901 ccacgcgcgt ggccgatatc gtcgccgacg ccgtgggtct cgccgaactc gaagcgcgca
     6961 agcgcaagat tcgcatcgtg accgatctgc gctcgcgttt gccggtgatc tacgtcgatc
     7021 cggtgctgat cgagcaggtc ttggtgaacc tgctgaagaa cggcgcggaa gcgatgcacg
     7081 atgcgcgccc gaatgcggtc gaccccgtga tccgcgtggt cgtgcggctg gaaggcgggt
     7141 ttgtctgcat cagcgtcgtc gaccaggggc cgggcgtcga cgaagcgacc gccgagcgtc
     7201 tctttgaacc gttttacagc accaagtccg atggtatggg catggggctg aacatttgcc
     7261 gttcgattat cgaatcgcac cgcggccgtc tgtgggtggt gaacaacgtc gaatctgacg
     7321 gccacatcac aggtgccacg ttccattgca gtctgcctat tggagagcct gacggcccga
     7381 gcaacggcgg gtcagaggcg ccgacaccac aaaccgtt
//
LOCUS       DATASH010000327         5244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4992676,
            whole genome shotgun sequence.
ACCESSION   DATASH010000327 DATASH010000000
VERSION     DATASH010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5244
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4992676"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            229..1479
                     /locus_tag="VHV99_09125"
     CDS             229..1479
                     /locus_tag="VHV99_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3635636.1"
                     /translation="MSTTDSKRFALPFNQTMLVVLAGALILSAAMGIRQTFGLFIGPF
                     SFDRGLPVTLIAFAIALHNLVWGFAQPFAGAAADRYGSAPVVAFGATTFAAGLGVAAV
                     APSGPMLIVGMGLLVGIGVSCTTFGVVLPAVGRIASPEQRSMAMGLVSAGGSAGQVLM
                     IPLAQSIRLNSGIATSLFVLAFLMLLIAPLGIVLDRRAGVGMQVGETPAVPLRQVVAQ
                     AARHRGYRLLTLGFFTCGFQLAFIATHLPEYLTLCHMPVGLGATALALIGLFNMAGSW
                     GCGWLGGRFRQQHVLGWLYLIRSVTIGAFFLLPKTSVSVVIFAAVMGLTWLGTVPLTS
                     GLVAKVFGTRHLGSLFGVCFLSHQIGSFLGAWLGGLVFDLTGSYSLLWEATVAAGLIA
                     AMLHFPIDDKAVSSPAIRSEPASA"
     gene            complement(1537..2142)
                     /locus_tag="VHV99_09130"
     CDS             complement(1537..2142)
                     /locus_tag="VHV99_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018434303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HEX3635637.1"
                     /translation="MANLQPISRYPVPEPSEWPDDISARILEVQQKAGFVPNVFLTLA
                     HRPDEFRAFFAYHDALMLKEGGLTKGEREMIVVATSAVNQCLYCVVAHGAILRIYEKA
                     PLLADQVAVNHRKADITARQKAMLDFALKVCRESGTVDDADFQTLREQGFSDEDVWDI
                     AAITAFFGLSNRMANVISMRPNDEFYLMGRVPKAAVDAPQK"
     gene            2413..3432
                     /locus_tag="VHV99_09135"
     CDS             2413..3432
                     /locus_tag="VHV99_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX3635638.1"
                     /translation="MWHFPVAIPPSLGVWAVFMSVLITQLGMPIPAAPMLILGGTMAA
                     TGQTSYASVVAAAVGATMIADALWFFTGRAYGRRLLNNLVRFSLSLDTTVRVARNTYE
                     RYGAPILTVAKFLPGLGLISAPLLGTTPINVGVFLLWDFVGAVLWAGAWVIGGAALHD
                     QIVQLVLLVRHNGGTIFDAFAAIFVAVLLYRCIRRWQFRRWLAHVRISPEQLDEMMNS
                     NEPPLILDARPRSIRAQESHRIAGAQVLDLDSPEPLHPELLKRPIVVYCVCPNEATAK
                     RIVNQLHRKNIHHVKALKGGLDAWEKRGYPVEPLPPDFETAMAHMSEDEGDEGEYTVR
                     ARLAK"
     gene            3429..3971
                     /locus_tag="VHV99_09140"
     CDS             3429..3971
                     /locus_tag="VHV99_09140"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3635639.1"
                     /translation="MTAGAAGGIRDGALYGLFPLFADAHGLGTTQTATLLTLFGVGGM
                     ALQFPVGWFADRASLAATVIVCAVLSTLAICGFAPASSLASASALLLGGMNSAYITLG
                     MVAAACSDKAALTRNMRLVSLTFTASSIVGPLIAGFAMNARGPDMLMWQLAIMSGALV
                     AYTLGLREGRRQPDRSSSMG"
     gene            complement(4024..4986)
                     /gene="speB"
                     /locus_tag="VHV99_09145"
     CDS             complement(4024..4986)
                     /gene="speB"
                     /locus_tag="VHV99_09145"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004525503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEX3635640.1"
                     /translation="MTELVYGDGAIRRSSLYGSSIENTYAGVLSFMRRKYTRELDGVD
                     VVVSGVPLDLATTFRSGARLGPAAVRAASVQLSELHPYPWGFNPFDDLAVTDYGDCWF
                     DAHNPLTIRESIVDHARTILRSDAKMLTLGGDHYITYPLLIAHAEKYGKPLSLIHFDA
                     HCDTWADDSPDSLNHGSMFYKAVKDGLIDPKTSVQVGIRTWNDDFMGINILDAAWVHE
                     HGARAAVERITSIAGERPAYLTFDIDCLDPAFAPGTGTPVAGGLSSAQGLAIVRGLGA
                     LNLVGADVVEVAPAYDQSEITALAAAHIACDLLCLWRQRKVAER"
     gene            complement(5121..>5244)
                     /locus_tag="VHV99_09150"
     CDS             complement(5121..>5244)
                     /locus_tag="VHV99_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010108095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX3635641.1"
                     /translation="EGGDMNFPFGGYKQSGNGRDKSLHALEKYTELKSTLVRLR"
BASE COUNT          893 a         1689 c         1610 g         1052 t
ORIGIN      
        1 gcggatagcg cggagcatcg tccggagctg tccgatttca agcgatggtt gctcagcgag
       61 gctgcgccgt tcgaggattc atccggtgcc cccaacgcgg tactgcgcag cgccgaccac
      121 tgaaccccgc atttccccac aggtgagcca cgctcacctg tggtcaaaat cttttctttt
      181 gccgcttctg acgctcgctg cgaccatggt gtccaaggag atcagaacat gtccaccacc
      241 gatagcaaac gcttcgccct cccgttcaat cagacgatgc tcgtggtgct cgccggcgcg
      301 ctgattctga gcgccgcgat gggcatccgg cagaccttcg gcctcttcat cgggccgttc
      361 tcgttcgacc gcggcctgcc cgttacgctg atcgctttcg ccatcgcgct gcacaacctg
      421 gtgtggggct tcgcgcagcc gttcgcgggc gccgctgcgg atcgctacgg ctcggcgccg
      481 gtggtcgcat tcggcgcgac gacattcgca gcgggcctgg gcgtcgcggc ggtggcgcca
      541 tcggggccga tgctgatcgt cggcatgggt ctgctggtcg gcatcggcgt gagttgcacg
      601 accttcggcg tggtattacc tgcggtaggc cgcatcgcct ccccggaaca acgcagcatg
      661 gcgatggggc ttgtcagcgc gggcggctcg gcgggacagg tattgatgat cccgctcgcg
      721 cagagcattc gactcaactc gggcatcgcc acgtcgctct tcgtgctggc cttcctgatg
      781 ctattgatcg cgccgctagg catcgtgctg gaccggcgcg cgggtgtcgg catgcaggtc
      841 ggcgagacgc ctgccgtgcc gcttcgccaa gtcgttgctc aagcggcccg gcatcgcgga
      901 tatcgactcc tgacgctcgg cttcttcacc tgtggatttc aactcgcgtt catcgccacc
      961 catttgcccg aatatctgac gctctgtcac atgcctgtcg ggttgggcgc aaccgcgctc
     1021 gcgctgatcg gcctgttcaa catggcgggc agttggggat gcggctggct cggcggacgg
     1081 ttccggcagc agcacgtact tggctggctt tacctcattc gaagcgtgac gatcggcgcc
     1141 ttctttctcc tgccgaagac ctccgtttcc gtcgtcatct tcgcggccgt gatgggactg
     1201 acctggctcg gcaccgtgcc tttgacgagc gggctcgtcg cgaaggtgtt cggcacgcgg
     1261 cacctgggca gtctgttcgg ggtctgcttt ctgagccatc agatcggctc gtttctgggt
     1321 gcgtggctcg gcgggctggt gttcgatctg accggttcgt attcgctgct ctgggaagca
     1381 accgtcgccg ccggactcat cgccgcgatg ctgcattttc cgatcgacga caaggcggtc
     1441 agttcgcctg cgatacggtc cgagccggcg agcgcttgag gactcgcaag cacgcatccg
     1501 cacgcgcgcc gcgaatcccc accccgcgcc ccaacgctat ttctgcggcg cgtccaccgc
     1561 cgccttcggc acgcgcccca tcaaatagaa ctcgtcgttc ggacgcatcg aaatgacatt
     1621 ggccatccgg ttcgacaagc cgaaaaatgc cgtgattgcc gcaatatccc acacgtcttc
     1681 atccgaaaaa ccctgctcgc gcagggtctg aaaatcggca tcgtcgaccg tgccggattc
     1741 gcggcagacc ttcagcgcga aatcgagcat cgccttctgg cgcgccgtga tatccgcctt
     1801 gcgatgattg acggccacct gatccgcaag caacggcgcc ttttcgtaaa tacgtagaat
     1861 tgcgccgtgc gcgaccacgc aatacaggca ctgattcacc gcgctcgtgg caacgacgat
     1921 catctcgcgt tcgcctttgg tcaagccgcc ttctttcaac atcaacgcgt cgtgatacgc
     1981 aaaaaaggcg cggaattcgt ctggccggtg cgccagcgtc aggaatacgt tcggcacaaa
     2041 gccagccttc tgctggacct cgagaatgcg ggcgctgatg tcgtccggcc attcgctcgg
     2101 ctcaggcacg ggatagcggc tgatcggctg aaggtttgcc atgcggtgtc tccgttatgt
     2161 ctgtatgtgc acgatgtgca cgacgattgt agcaatgtgg gctttcagcc ttcccggcag
     2221 cgccgttttg ccaacctgtg cttaaatccg tgtcggcgta ctgcattcgt gccgcctcgc
     2281 gcgacgggtg cgcgtccgcc cgatggccgg caggaacgga acacgcggtc cgggttcgtg
     2341 ccggtgtcga tcgggcgcca actgggcgcg aacacattca aatcccttca ttccatcaag
     2401 aggagcgcgg ttgtgtggca ttttccggtt gctattccac cctcgctggg cgtgtgggcc
     2461 gtcttcatga gcgtgctgat cacgcagctc ggcatgccga ttccggcggc gcccatgctg
     2521 atcctcggcg gaacgatggc ggcaacggga cagacctctt acgccagtgt ggttgccgcc
     2581 gctgtcggtg cgacgatgat tgcggacgcg ttgtggttct tcacggggcg cgcttacgga
     2641 cggcgcttgc tgaacaacct ggtgcgcttc tcgctctcgc tcgacaccac cgtgcgcgtc
     2701 gcgcgcaaca cctacgaacg gtacggcgca ccgattctca ccgttgcgaa attcctgccc
     2761 ggactcggcc tgatctcggc gccgctgctc ggcacgacgc caatcaacgt cggcgtgttt
     2821 ctgctgtggg attttgtcgg cgcggtgcta tgggccggcg cgtgggtgat cggcggcgcg
     2881 gcgcttcacg atcagatcgt gcaactcgtt ctgctcgtgc gccataacgg cggcacgatt
     2941 ttcgatgcgt ttgcggcaat cttcgttgcc gtgctccttt atcgctgcat acgccgctgg
     3001 cagtttcgcc gctggctcgc gcatgtgcgc atctcgccgg aacagctcga cgaaatgatg
     3061 aactccaacg agccaccgct gattctcgat gcgcggccgc gcagcatacg cgcgcaagag
     3121 tcgcaccgga ttgccggcgc gcaggtgctg gatctcgatt cgcccgagcc gctgcatccc
     3181 gagctactga agcggccaat tgtcgtttat tgcgtctgtc ctaacgaagc gaccgccaag
     3241 cggatcgtca atcagttgca tcgaaagaat atccaccatg taaaagcgct caagggcggc
     3301 ctcgacgcct gggaaaagcg cggctatccg gtcgaacccc tgccgcccga cttcgagacg
     3361 gcgatggccc atatgtcgga agacgaagga gacgagggcg aatacaccgt gcgggcgaga
     3421 ctggcgaaat gacggcgggc gccgcaggcg gcatcagaga tggcgcgctc tacggcctct
     3481 tcccgctgtt cgccgacgcc cacggtctcg gtacgacgca gacggccacg ctactcacgc
     3541 tattcggcgt cggtggcatg gcgctgcaat ttccggtcgg ctggttcgcc gatcgcgcga
     3601 gcctcgccgc cacggtgatc gtctgcgcgg tgctcagtac gctggcaatc tgcggtttcg
     3661 cgcccgcctc atcgctcgcc tcagcgagcg ccctgctgct tggcggcatg aacagcgcgt
     3721 acatcacact cggtatggtc gcagctgcat gcagcgataa agccgcgctg acgcgcaaca
     3781 tgcggctggt atcgctgact ttcactgcaa gttcgatcgt cgggccgctc atcgcaggct
     3841 tcgcgatgaa cgcgcgcggc cccgatatgc tgatgtggca actggcgatc atgagcggcg
     3901 cgctcgtggc ctacacgctc ggcttgcgcg aaggtcggcg tcaacccgat cgatcgtcgt
     3961 cgatgggctg agcctggatc aagccgcttg tgtttcaagc cgcttgatgt ggatggaacg
     4021 agcctaacgt tcagccactt tccgctgccg ccacagacac aacagatcgc aagcaatgtg
     4081 cgcggcggca agcgccgtga tctcgctctg gtcataggcg ggcgccactt ccaccacatc
     4141 cgcgcccacc agattcaacg caccgagccc acgcacaatg gcgagtccct gtgccgacga
     4201 caaaccgccc gccaccggcg tacccgtgcc cggcgcgaag gccggatcga gacagtcgat
     4261 atcgaaggtc agatacgcgg gccgctcgcc tgctatcgac gtgatccgct ccactgccgc
     4321 gcgtgcgcca tgttcatgaa cccacgcggc gtcgaggata ttgatcccca tgaaatcgtc
     4381 gttccacgtg cgaatgccga cttgtactga ggtcttcgga tcgatcaaac cgtccttcac
     4441 cgctttgtag aacatcgagc cgtgattcag gctgtccgga ctgtcgtcgg cccacgtatc
     4501 gcagtgggcg tcgaaatgaa tcagcgagag cggcttgccg tatttctccg catgcgcaat
     4561 cagcaacgga taagtgatgt agtgatcgcc accgagcgtc agcatcttcg cgtccgaacg
     4621 caggatcgtg cgcgcatgat cgacgatcga ttccctgatc gtcagcggat tgtgcgcgtc
     4681 gaaccagcag tcgccgtaat cggtcacggc gagatcgtcg aacgggttga agccccacgg
     4741 atacggatgc agctccgaca attgcacgct cgcggcgcgc accgccgccg ggccgagacg
     4801 cgcgcctgag cggaaagtgg tggccagatc gagcggcacg cctgagacca cgacgtcgac
     4861 accatcgagt tcgcgggtgt acttgcggcg catgaacgac aacacacccg cataggtgtt
     4921 ttcgatcgac gagccataga gcgacgaacg gcggatcgcg ccgtcgccgt aaacgagttc
     4981 agtcatagat gacctgatac ctgataaaca tgccggcgcc gcaggcgggg ttccagggga
     5041 tgcgtgcgac gctggcctcg gattcattag gaacgcggca gccgcgttcg tgcgaaccgc
     5101 cgcgctggct tgcgaatcct ttagcgcagc cgcaccagcg tggacttcag ctcggtgtac
     5161 ttctccagcg cgtgcaatga cttgtcgcgg ccattgcccg attgcttgta gccgccaaac
     5221 gggaagttca tatccccgcc ttcg
//
LOCUS       DATASH010000328         3072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3213148,
            whole genome shotgun sequence.
ACCESSION   DATASH010000328 DATASH010000000
VERSION     DATASH010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3213148"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..434
                     /locus_tag="VHV99_09155"
                     /pseudo
     CDS             141..434
                     /locus_tag="VHV99_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071503.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
     gene            785..1387
                     /gene="cobO"
                     /locus_tag="VHV99_09160"
     CDS             785..1387
                     /gene="cobO"
                     /locus_tag="VHV99_09160"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX3635642.1"
                     /translation="MKTDPESHQRMTERRREGHEKKQASATVEKGLLIVNTGTGKGKT
                     TAAFGMAVRVLGHGMRLGVVQFIKGALHTSERDFLGAIANCDFVTMGDGYTWNTQNRD
                     ADIATARKGWNEARRMIESGDYQMVILDELNTVLKYEYLPLDEVLSVLNARAQMLHVV
                     VTGRHAPDALIEAADLVTEMRIVKHPYREQGVKAQRGVEF"
     gene            1391..2770
                     /locus_tag="VHV99_09165"
     CDS             1391..2770
                     /locus_tag="VHV99_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HEX3635643.1"
                     /translation="MSTSTRSTSYGKSPEGTSFGTAAKGTSVGTCPALFISAPASGQG
                     KTTITAGLARYHRRLGRRVRVFKTGPDFLDPMILARASGAPVLSLDLWMVGEPACRNL
                     LAQAAAEADLILIEGVMGLFDGTPSSADLATTFGVPVLAVISAKAMAQTFGAVAFGLA
                     QFRPQVPFYGVLANRVGSARHAQMLEEALPPDLRWCGHVTGTDEIGLPDRHLGLHQAD
                     EIADLDARLDRAADALAQTALAELPPPVEFGAPVPEALPRLLAGKRIAIARDAAFSFI
                     YPANVMLLEALGAHVDYFSPLADEALPQHADALYLPGGYPELHAALLATSTRSAATIR
                     AHAAAGKPLVAECGGMLYLLDQLTDTHGTTTPMLGLLPGHATMQPRFTSLGMQQIDGL
                     HGPVTGHTFHYSKLTTSLAPLRSATRPHSDAPGEAVFRAGSIVATYMHAYWPSNPAFT
                     AALFHGEAF"
     gene            2754..>3072
                     /locus_tag="VHV99_09170"
     CDS             2754..>3072
                     /locus_tag="VHV99_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX3635644.1"
                     /translation="MAKPFNDSERDAVYRAIYERRDMRHFVRDPIDPAVLQRLLDAAH
                     HAPSVGFMQPWRIARITDPALRTALHGSVESERLATADALGKRREEFMKLKVEGMLEC
                     GELL"
BASE COUNT          573 a         1068 c          920 g          511 t
ORIGIN      
        1 gcggtgggtg cggcaaacat ggcgaactct gccggagcga cggatgcttc ggatgcttcg
       61 gatgcttcgg acgcttcgga cgcttcggac gcttcggacg cttcggacgc ttcggacgct
      121 tcggacgctt cggacgcgca atgaaaacac taaccgtcgg catcggttgt cgcttgtata
      181 gctcggccga gcagatcgaa gcggctgtgc gtgccacact cggctcgcat gccttcgatg
      241 aaatcagcac gattgcctcg gtcgacgcca aagcccacga agccggtttg ctcgaattct
      301 gcgcacgtca tgcgttgccg ctgaaattgt tcagccgtga gcagattgcg gcgatatccg
      361 tggaccatcc atcggcggcc gcgcgcgagt atctcggcgt agacggcgtg tgcgagccgt
      421 gtgccttact cgccgcgctg gccgaatctg atgcggccag cacgcctgat gccgcccatg
      481 catcggtcgc gtcagctgcc ttagatgcct cagatgccgc agttacccca aatgcggcag
      541 ttgccccaga tgccgcagtt accccagatg ccgcagttac cccaaatgcg gcagttaccc
      601 caaatgcggc agttacccca gatgcgccag ttgccctact tgactccatc gcgcccgccc
      661 cttccaacgc acgcctgctc gcgcgcaaaa ccgtccacaa cggcatcacc gtggcgatat
      721 catcgaccac cgcgcgcccc cccagcgaac agacaaacac cgaccctcac caacaggatc
      781 tgccatgaag accgaccccg aatcgcatca acgcatgacc gagcgccggc gcgaaggcca
      841 cgagaaaaag caggccagcg ccaccgtcga aaaaggtctg ctgatcgtca acaccggcac
      901 cggcaaaggc aagaccacgg cagcgttcgg catggccgtg cgcgtgcttg gacacggcat
      961 gcgcctcggc gtcgtgcaat tcatcaaagg cgcgctgcat acctcggagc gcgacttcct
     1021 cggtgccatc gccaattgcg acttcgtcac gatgggcgac ggctacacct ggaacacgca
     1081 gaatcgcgac gccgacatcg ccaccgcgcg caaaggctgg aacgaagcgc gccgcatgat
     1141 cgaaagcggc gactatcaga tggtgattct cgacgaactg aacacggtgt tgaagtatga
     1201 atacctgccg ctcgacgaag tgctgtcggt tctgaatgcg cgggcgcaga tgctgcacgt
     1261 ggtggtaacc ggccgccacg cgccggacgc gctgatcgaa gccgccgacc tcgtcaccga
     1321 aatgcgcatc gtcaaacatc cgtatcgcga gcaaggcgtg aaagcgcaac gcggcgtgga
     1381 gttctgagcc atgtccacgt ccaccaggag tacgtcctac gggaagtccc ccgagggaac
     1441 gtccttcggg acggccgcca agggcacttc cgtcgggacg tgccccgccc tgttcatcag
     1501 cgcgccggcg tcgggtcaag gcaagaccac gatcaccgcg ggtctcgcgc gctatcaccg
     1561 gcggctcggg cgacgcgtgc gcgtcttcaa gaccggcccc gactttctcg acccgatgat
     1621 tctggcgcgt gccagcggcg caccggtcct gtcgctcgat ctgtggatgg tgggcgagcc
     1681 ggcctgccga aacctgctcg cgcaagcggc ggccgaagcg gacctgatcc tgatcgaagg
     1741 cgtgatgggt ctgttcgacg gcacaccgag cagcgcagac ctggcaacca cgttcggcgt
     1801 gccggtcctc gcggtgattt cggcgaaagc gatggcgcag accttcggcg cggtcgcctt
     1861 cggcctcgcg caattcaggc cgcaagtgcc gttttacggc gtgctcgcca atcgcgtcgg
     1921 ctcggcaagg cacgcgcaaa tgctcgaaga agcgttgccg ccggatctgc gctggtgcgg
     1981 ccatgtcacc ggcaccgacg aaatcgggtt gcccgatcgc catctaggct tgcatcaggc
     2041 cgacgagatc gccgatctgg acgcccgcct cgaccgcgcc gccgatgcgc tcgcgcaaac
     2101 cgcgctggct gaattgccgc cgccggtcga attcggcgca cctgtgcccg aagcgctgcc
     2161 gcgtcttctg gccggcaagc gcatcgccat tgcgcgcgat gccgcgttct cgttcatcta
     2221 tccggccaac gtcatgctgt tagaggcgct cggcgcgcac gtcgactatt tctcaccgct
     2281 agccgacgaa gccctgccgc agcacgccga cgcgctatat ctcccgggcg gctatcccga
     2341 actgcatgcg gcattgctag caaccagcac gcgcagtgcc gcaaccatcc gcgcgcacgc
     2401 cgcagcaggc aaaccgctcg ttgccgaatg cggcggcatg ctctacctgc tcgaccaact
     2461 caccgacacg cacggcacca cgacgccgat gctcggcctg ctgcccggcc atgcgacgat
     2521 gcaaccgcgc tttacctcgc tcggcatgca acagatcgac ggtctgcacg gcccagtgac
     2581 cggccacacc ttccactatt cgaagctgac gacgtcgctc gcgccgctgc gttcagccac
     2641 ccggccgcat agcgatgccc ccggcgaggc cgtgttccgc gccggctcga tcgtggcaac
     2701 ctatatgcat gcttattggc cctccaaccc ggccttcacg gccgccctgt tccatggcga
     2761 agccttttaa cgattccgag cgcgacgcag tctatcgcgc gatttacgaa cgccgcgaca
     2821 tgcgccattt cgtgcgcgat ccgatcgacc ctgccgtgtt gcaacgcctg ctcgacgcgg
     2881 cgcatcacgc gccgagcgtc ggcttcatgc agccgtggcg catcgcgcgc atcaccgacc
     2941 cggctctgcg cacggccttg cacgggagcg tcgaaagcga acgcctcgcg accgccgacg
     3001 cactcggcaa acgtcgcgaa gaattcatga agctgaaagt ggaaggcatg ctggaatgcg
     3061 gcgaactgct gg
//
LOCUS       DATASH010000329         1795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5980961,
            whole genome shotgun sequence.
ACCESSION   DATASH010000329 DATASH010000000
VERSION     DATASH010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5980961"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            329..>1795
                     /gene="xdhA"
                     /locus_tag="VHV99_09175"
     CDS             329..>1795
                     /gene="xdhA"
                     /locus_tag="VHV99_09175"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase small subunit"
                     /protein_id="HEX3635645.1"
                     /translation="MTEPIRFYHRNAIREIKDAPVTRTVLQYLREDAHCTGTKEGCAE
                     GDCGACTVVIGERNAAGGVDFKAVNACIQFVPTLDGKALFTVEDLRQPDGSLHPVQEA
                     MVECHGSQCGFCTPGFVMSMWSLYEKHGHEHSCANKTVPSRDAISNALTGNLCRCTGY
                     RPIVDAAVRMFEAPAPKAPVNIEALSKTLATLERQDTFHYQHAGQQFAAPRTVDALAK
                     IKEAEPATRILAGSTDIGLWVTKLMRELGNIVYVGQIAELQKLETNDDWIEIGAGISV
                     ERAYAEIAKQYPELNEMWQRFASLPIRNAGTLGGNIANGSPIGDSMPGLIALGAHVIV
                     RGGEIEREMPLEDLYLAYQKKDMAEHEFVVGLKVPTRTGARKNLQFRTYKLSKRFDSD
                     ISAVCAAFSFIADGNVIREPRIAFGGMAATSKRATHAEAVLRDAEWHEATAQAAMLAL
                     GNDYAPLSDMRATSNYRLEAAKNTLYRFWLETRPNNPLP"
BASE COUNT          397 a          565 c          527 g          306 t
ORIGIN      
        1 caggagttgc ttaataattt cgccggcgaa aaaaaggtgc cggagtcata tagtgattcg
       61 ttttctccct gcatgagcgg tctttccatg cgcagcgtat gaacagaata gggccgcttg
      121 tggcaataac gagatagtta acaacaaaat acggcaaaac agccaaatca acataagcca
      181 atcacccctc aaacccatct cgatatcgtc aagcggatat gtcccgcatg cggtccctgc
      241 tatgggttgt atttattgac ggtgatagtt ttcgaaccat catcgcagca cgtccctacg
      301 gacacagaac attcgagagt aactagacat gactgagccg atccgcttct accaccgcaa
      361 cgcgatccgc gagatcaagg acgcgcccgt gacccgcacc gtcctgcagt atctgcgcga
      421 ggacgcacat tgcaccggca ccaaggaagg gtgcgccgaa ggcgactgcg gcgcatgcac
      481 ggtcgtgatc ggcgagcgca acgcggcggg cggcgtcgac ttcaaggcgg tgaacgcctg
      541 catccagttc gtgccgacgc tggacggcaa agccctcttc accgtcgaag atctgcgtca
      601 gccggacggc tcgctgcatc cggtgcagga agcgatggtc gagtgccacg gttcgcagtg
      661 cggtttctgc acgccgggct tcgtgatgtc gatgtggtcg ctgtacgaaa agcatggcca
      721 cgaacacagc tgcgcgaaca agaccgtgcc gtcgcgtgac gcaatcagca acgcgctgac
      781 cggcaacctg tgccgttgca ccggctatcg tccgatcgtc gacgcagcag tgcggatgtt
      841 cgaagcgccc gcgccgaagg cgcctgtcaa tatcgaagcc ctgtcgaaaa cgctcgccac
      901 gctcgaacgc caggacacgt tccactatca gcatgccggc cagcaattcg ccgcgccacg
      961 caccgtcgac gccctcgcga aaatcaagga agccgaaccg gctacccgca ttctcgccgg
     1021 cagcacggac atcggcctgt gggttaccaa gctgatgcgt gagttgggca acattgtcta
     1081 cgtcggccag atcgccgagc tgcaaaagct cgaaacgaat gacgactgga tcgagatcgg
     1141 cgcgggcatc agcgtcgaaa gggcgtacgc ggaaatcgcg aagcagtacc cggaactcaa
     1201 cgaaatgtgg caacgcttcg cgtcgctgcc gattcgcaat gccggcacgc tcggcggcaa
     1261 catcgccaat ggctcgccga tcggcgattc gatgccgggc ctgatcgcgc tcggcgcgca
     1321 tgtgatcgtc cgcggcggcg agatcgaacg cgaaatgccg ctcgaggatc tgtacctcgc
     1381 gtatcagaag aaggatatgg cggagcacga gtttgtcgtc ggcctcaagg tgccgacgcg
     1441 caccggcgcg cgcaagaatc tgcaattccg cacctacaaa ctgtcgaagc gtttcgactc
     1501 ggacatctcg gccgtgtgcg ccgcgttctc ctttatcgcc gacggcaacg tgatccgcga
     1561 gccacgcatc gcattcggcg ggatggccgc cacctcgaag cgtgcgacgc acgccgaagc
     1621 cgtgctgcgc gatgccgaat ggcacgaagc cacggcacag gccgcgatgc tcgcgctcgg
     1681 caacgactac gcgccgctgt ccgacatgcg cgcgaccagc aactatcgcc tcgaagcggc
     1741 gaagaacacg ttgtaccgct tctggctcga aacgcgtccg aacaatccgc tgccc
//
LOCUS       DATASH010000330         4099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2025199,
            whole genome shotgun sequence.
ACCESSION   DATASH010000330 DATASH010000000
VERSION     DATASH010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4099
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2025199"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VHV99_09180"
     CDS             <1..302
                     /locus_tag="VHV99_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HEX3635646.1"
                     /translation="RYVLMPTIFKHIRALKPGAGGELQLTDAVQSLLTEEQVLAYRYF
                     GTRFDCGSKLGYLKATVEFALRHPEVKADFEAYLQTYLQANMQASVPAQYTAEAA"
     gene            complement(498..2672)
                     /locus_tag="VHV99_09185"
     CDS             complement(498..2672)
                     /locus_tag="VHV99_09185"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HEX3635647.1"
                     /translation="MTQMNTRGGLQVAANLDQFVETEALPGTGLDSAAFWSGLDALVH
                     ELAPRNRALLAERDRLQTELDNWHRANPGPVRDLRAYRAFLEGIGYIVPAPASVKATT
                     DQVDTEIAEQAGPQLVVPLSNQRYALNAANARWGSLYDALYGTDAIPETNGAEKQKAF
                     NPVRGAAVIAYARKFLDQAAPLANGSHVDATLYSVEAGKLIVTLKNGKTELKAPAQFI
                     GYQGEASAPSAVLLKHNGLHFEIQIDANDSIGKTDSARVKDVLVEAAVSTIIDCEDSV
                     AAVDAGDKVQLYRNWLGLMNGDLTEEVTKNGKTFTRRLNADRVYTAANGTAPVVLHGR
                     SLLFIRNVGHLMTNPAVLTQDGQEIPEGILDAVITSLCSLHDRKHKLNSRTGSIYIVK
                     PKMHGPAEVAFASELFARVEDLLKLPRNTIKMGIMDEERRTSVNLLACIGEASARVAF
                     INTGFLDRTGDEMHTAMQAGPMMRKGDMKSSAWIAAYERSNVLVGLSAGLRGRSQIGK
                     GMWAMPDLMHAMLEQKIAHPKAGANTAWVPSPTAATLHAMHYHQVDVQAVQQELERTD
                     YSKVRDELLDGLLTIPVVESAKWSADEIRSEVDNNAQGILGYVVRWIDQGVGCSKVLD
                     IHNVGLMEDRATLRISSQHIANWLYHGVVTREFVEETFKRMAKVVDEQNAGDPLYKPM
                     APGFDTIAFKAAQALVFEGRQQPSGYTEPLLHKFRLEVKQEQ"
     gene            complement(2819..3223)
                     /locus_tag="VHV99_09190"
     CDS             complement(2819..3223)
                     /locus_tag="VHV99_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX3635648.1"
                     /translation="MLSKPVLTVAETTRILEAARAEAEKNQWAVAIVVVDDGGHPLGM
                     LRLDGSAPVSSYIATEKARTSALGRRETKDYEDMINNGRTAFLSAPLQGTLEGGVPVI
                     VDGQVVGAVGVSGVKAAQDAQIAKAGIQAIVG"
     gene            complement(3243..>4099)
                     /gene="glcF"
                     /locus_tag="VHV99_09195"
     CDS             complement(3243..>4099)
                     /gene="glcF"
                     /locus_tag="VHV99_09195"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HEX3635649.1"
                     /translation="VRRKVPSRSALPKARPASRHQRTMLMLEGCVQPALSPNTNAAAA
                     RVLDRLGISIVNVERAGCCGATDYHLNAQEAGLARARRNIDAWWPAIEAGAEAIVQTA
                     SGCGAFVKEYGHLLRDDPRYASKAQRVSVLARDLVEVLTNEPLDQLQASQLPGHSPGD
                     LPANLPTQMPQQLTPQPRQKIAFHCPCTLQHAQKLGGAVESILQRLGFDLTAVPDGHL
                     CCGSAGTYSITQPELAERLRNNKLDALESGKPELIVTANIGCQTHLDGAGRTPVRHWI
                     ELVEASLP"
BASE COUNT          690 a         1270 c         1286 g          853 t
ORIGIN      
        1 ggcgctacgt gctgatgccg accatcttca agcatatccg cgcgttgaag ccgggtgcag
       61 gcggtgaatt gcaattgacg gatgcggtgc agtcgctgct gaccgaagag caggtgctcg
      121 cctatcgcta cttcggcacg cgcttcgatt gcggcagcaa gctgggctat ctgaaggcga
      181 cggtggaatt cgcgctgcgt catccggaag tgaaggcgga tttcgaagcg tatctgcaaa
      241 cgtatctgca ggcgaatatg caggccagtg taccggccca gtacacggct gaagcggctt
      301 aaggtgactt aaggtgactt aaggcggctt cggcggcagg gcgtcgcaca tgcttcacgc
      361 cgaagcaacg cgcagtactt ccgcgcaaat taaaacgggc gccgcatcat gcggcgcccg
      421 tttttcttat cggcctctac ccgggcgagc ccgaaagatc atcgagcctt atacccagac
      481 tgcggctcaa taagccctta ctgctcctgc ttcacttcca accggaactt gtgcagcagc
      541 ggttccgtat agccgctcgg ctgctggcgt ccttcgaaca ccagcgcttg cgcggccttg
      601 aacgcgatcg tatcgaagcc gggcgccatc ggtttgtaga gcgggtcgcc cgcgttctgc
      661 tcgtcgacca ccttcgccat acgcttgaag gtctcttcga cgaactcgcg cgtcaccaca
      721 ccgtgataca gccagttggc gatgtgctgg ctggaaatac gcagcgtggc acggtcttcc
      781 atcaagccga cgttgtgaat gtccagtacc ttcgagcaac ccacgccctg gtcgatccag
      841 cgcaccacgt aaccgagaat gccctgcgcg ttgttgtcga cttcgctgcg aatctcgtct
      901 gcgctccact tcgccgattc gaccaccgga atcgtcagca ggccgtcgag cagttcgtcg
      961 cgcaccttcg agtaatccgt gcgttcgagt tcctgctgaa ccgcctgcac gtcgacttga
     1021 tggtaatgca tcgcatgcag cgtcgcggcg gtcggcgagg gcacccatgc ggtattcgca
     1081 ccggccttcg gatgcgcgat cttctgttcg agcatcgcgt gcatcaggtc gggcatcgcc
     1141 cacatgccct tgccgatctg cgagcggcca cgcaggcccg cgctcaaacc gaccagcacg
     1201 ttgctgcgct catacgcggc aatccatgcg ctcgacttca tgtcgccctt gcgcatcatc
     1261 gggccggctt gcatcgccgt gtgcatttcg tcgccggtgc ggtcgaggaa acccgtgttg
     1321 atgaacgcga cacgtgccga tgcttcgccg atacacgcga gcaggttgac ggaggtgcgg
     1381 cgctcctcgt ccatgatgcc catcttgatc gtgttgcgcg gcagcttgag cagatcttcg
     1441 acgcgcgcga acagctcgct cgcgaacgcg acttcggccg ggccgtgcat tttcggcttc
     1501 acgatataga tcgagccggt gcgcgaattc agcttgtgct tgcggtcgtg cagcgagcac
     1561 agcgaagtga tgacggcgtc gagaatgcct tccggaattt cctggccatc ctgggtgagc
     1621 acggccggat tcgtcatcaa atgaccgacg ttgcggataa acagcagcga gcggccatgc
     1681 agcacgaccg gcgcggtacc gtttgcggcg gtgtacacgc gatcggcatt cagacggcgg
     1741 gtgaaagtct tgccgttctt cgtgacttct tcggtcaggt cgccgttcat caggccgagc
     1801 cagttgcgat agagctggac cttgtcgccg gcatcgacgg cggcaaccga gtcttcgcag
     1861 tcgatgatcg tgctcaccgc ggcttcgacc agcacgtcct tcacgcgcgc cgaatcggtc
     1921 ttgccgatcg aatcgttcgc gtcgatctgg atttcgaaat gcaggccgtt gtgcttcagc
     1981 agcacggccg acggtgcgct cgcttcgccc tgatagccga tgaattgcgc cggcgccttc
     2041 agttcggtct tgccgttctt tagcgtgacg atgagcttgc cggcttcgac gctatacagc
     2101 gtggcgtcga cgtgcgagcc gttcgcgagc ggggcggcct gatcgagaaa cttgcgggca
     2161 taagcgatca ccgcggcgcc gcgcaccgga ttgaaggctt tctgcttttc ggcgccgttg
     2221 gtctcgggga tcgcatcggt gccgtacagc gcgtcgtaca ggctgcccca acgcgcgttg
     2281 gcggcgttga gcgcatagcg ctgattcgac agcggcacga cgagttgcgg gccggcctgt
     2341 tcggcgattt cggtgtccac ctggtcggtc gtggctttga cgcttgccgg cgccggcacg
     2401 atgtagccga tgccttcgag gaaggcgcgg tacgcgcgca ggtcgcgcac cggacccgga
     2461 ttggcgcgat gccagttgtc gagttcggtt tgcaggcggt cgcgctcggc cagcagcgca
     2521 cggttcctgg gcgccagctc gtgcacgagg gcgtcaagac ctgaccagaa tgcggcgctg
     2581 tcgagtccgg tgccgggcag ggcttcggtt tcgacgaact ggtcgaggtt ggcggcgact
     2641 tgaagtccac cgcgcgtgtt catctgagtc atcaactgct ccatctgaat gggaccgatg
     2701 agggggaatc ggtccgcgtt gtgttcgtta ttgtgctgcg aatttattgc cgccgctttg
     2761 aacgcaatct gaaacgaaaa tacgcacgaa atgctagttt aagcggcgat gggcaaaatt
     2821 aaccgacgat agcctgaatt ccggccttgg cgatttgcgc gtcctgagcc gccttgacgc
     2881 ccgacacgcc gaccgcgccg accacctggc cgtcgacgat caccggcacg ccgccttcca
     2941 gcgtgccttg caacggcgcg ctcaggaatg ccgtgcggcc gttgttgatc atgtcttcat
     3001 agtccttcgt ttcgcgacgg ccgagcgccg acgtgcgggc tttttccgtt gcgatgtacg
     3061 agctgaccgg cgcgctgccg tccagacgca gcatgccgag tgggtggccg ccgtcgtcga
     3121 ccaccacgat ggcgacggcc cattggttct tctcggcttc cgcgcgggcg gcctcgagga
     3181 ttcgggtcgt ttcggcaacg gtcaacacgg gtttgctcag cataatgaat ttcctgtttc
     3241 gtttaaggta aggatgcttc tacaagttct atccaatggc gtaccggcgt acggccggcg
     3301 ccgtccagat gcgtctggca accaatgttg gccgtgacga tcagctccgg cttgccgctt
     3361 tccaacgcat cgagcttgtt gttccgtaat ctttccgcaa gctccggctg ggtgatcgaa
     3421 tacgtgcccg ccgatccgca gcacaaatgg ccgtcgggca cggcggttag atcgaagccg
     3481 agccgttgca ggatcgactc gaccgcgccg cccagttttt gcgcatgctg caacgtgcat
     3541 gggcagtgaa acgcgatctt ttgccgcggc tgcggtgtca actgctgggg catttgcgtc
     3601 ggcaaattcg ctggcaaatc ccccggcgaa tgcccaggca actgactcgc ttgcaactga
     3661 tcgagcggct cgttagtcaa tacctcgacg aggtctcgcg ccagaacgct cacccgctgc
     3721 gcttttgacg catagcgagg atcatcgcgc agcaggtgcc cgtactcctt gacgaacgcg
     3781 ccgcagccgc tcgcggtttg cacgatggcc tcggcgccgg cctcgatggc gggccaccac
     3841 gcgtcgatat tgcgccgtgc ccgcgcgagg cctgcctctt gcgcattcag atgataatcg
     3901 gtcgccccgc agcagccggc acgctcgaca tttacgatgc tgatgcccag ccggtccagc
     3961 acgcgcgcgg cggccgcgtt ggtgttcggt gacagcgcgg gttgcacgca gccttccagc
     4021 atcagcatcg tgcgctggtg ccgcgacgcg ggccgggctt tcgggagggc gctgcggctg
     4081 gggactttac gtcggactg
//
LOCUS       DATASH010000331         4647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4003363,
            whole genome shotgun sequence.
ACCESSION   DATASH010000331 DATASH010000000
VERSION     DATASH010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4647
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4003363"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1131)
                     /locus_tag="VHV99_09200"
     CDS             complement(<1..1131)
                     /locus_tag="VHV99_09200"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEX3635650.1"
                     /translation="MTHPASEPNPPAAGTHTRDAITGDARAGAASSALEPILATAGVS
                     KTFPGVKALQRVDFRLFPGEIHTLMGQNGAGKSTLINVLTGVVAPDAGTIRLGGEIVA
                     FASPQEAEAAGVRTLYQEVNLCPNLSVAENIFAGRQPKRFGAIDWPDIKRRAQAALLR
                     LDVTLDVTRSLDAYPIAVQQMVAIARALSVDARVLILDEPTSSLDDGEVAQLFKILRH
                     LKQSGIAILFVTHFIEQTYAISDRITVMRNGEREGEYLARDLPADQLVAKMVGHERMS
                     ARLREAAHDGHTAHEGPQSKQSVQPFVELRGVGRRGTLQPIDLDVQPGQILGLAGLLG
                     SGRTETARLLFGADSADSGTILVEGRPVRVRSPRDAVRHGIGY"
     gene            complement(1357..2343)
                     /locus_tag="VHV99_09205"
     CDS             complement(1357..2343)
                     /locus_tag="VHV99_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3635651.1"
                     /translation="MANTRRAACRALGALALCSSIGALALTTPGAYAQDKQITLGFAQ
                     VGAESAWRTANTESVKSAAADAKIKLKFSDGQQKQENQIKAIRSYIAQKVDVIAFSPV
                     VESGWEPVLREAKAAKIPVILTDRNIDVKDTSLYVTMIGSDFLEEGRRGGHWLADHYK
                     NEPGPVNIAELQGTVGSAPANDRHAGLIEVIKSDPKFKIIASQSGDFTLAGGKQVMEA
                     FIKTYGNKINVVYAHNDDMALGAIQAMEEAGMHPGKDIVVVSFDATKGGFQAMAAGKM
                     NVDVECSPLLGPQLMSAVKDVVAGKPLPKRILTEETVFPMSVAAQTLPTRKY"
     gene            complement(2595..3200)
                     /locus_tag="VHV99_09210"
     CDS             complement(2595..3200)
                     /locus_tag="VHV99_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HEX3635652.1"
                     /translation="MADLFDDLPAPDVDWYPEWLAPAAAAHALTQLIDEVEWRQDMMG
                     TPAGRVPLPRLTAWQGEPDAVYVYSGIRNLPQSWTPTVAELRAAAEAACHARFNSVLI
                     NRYRSGTDSMGWHADREPELGARPVIASISLGVARTFDLRHNKTGVVQSFSLKGGSLL
                     VMKGDTQADWRHRVPKEPRVAGERINLTFRWVTPREATPST"
     gene            3345..3929
                     /locus_tag="VHV99_09215"
     CDS             3345..3929
                     /locus_tag="VHV99_09215"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HEX3635653.1"
                     /translation="MILSDQALDQLFREARTHNGWQAKPVDDALLRQLTQLVLLGPTS
                     ANSSPGRFVYVKTPEGKEKLRPALSAGNLEKTMAAPITVIVGMDMAFYEHLPKLFPHA
                     DARSWFAGNDKAIADTAFRNSTLQGGYLILAARALGLDTGAMSGFDAAKVDETFFAGT
                     SVRSNFLINLGYGDPSKLFPRSPRLSFDEAAQIV"
     gene            complement(3953..4291)
                     /locus_tag="VHV99_09220"
     CDS             complement(3953..4291)
                     /locus_tag="VHV99_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006053255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635654.1"
                     /translation="MKLLGSGNHVRHGHHAHHAHHVGREGSHVMLPLVGISLMALALY
                     FGVRDIDFSELGKSALFYVVMVCVALGIAGLFGVVGAWLRSNGDEAEEGFCFIGALIG
                     AVVFYVALFR"
BASE COUNT          839 a         1484 c         1405 g          919 t
ORIGIN      
        1 gtagccgatg ccatgacgca cggcgtcccg cggtgaacgc acccgcacag gccggccttc
       61 gaccaggatg gtgccgctgt cggcgctgtc cgcgccgaac agcaaccgcg cggtctccgt
      121 gcggcccgag cctagcagac cggcaaggcc caggatctgg cccggctgca cgtcgaggtc
      181 aatcggctgc agcgtaccgc gccgtccgac gccgcgcagt tcgacgaacg gctgaacgga
      241 ctgcttgctc tgcggtcctt cgtgcgcggt gtgcccgtca tgcgccgctt cgcgcaagcg
      301 cgcgctcatg cgttcgtgac cgaccatctt cgcgaccagt tgatcggccg gcagatcgcg
      361 tgcgaggtat tcgccttcac gttcgccgtt gcgcatcacc gtgatgcgat cggagatcgc
      421 atacgtctgc tcgataaaat gcgtcacaaa cagaatggcg atacccgatt gcttaagatg
      481 gcgaagtatc ttgaaaagct gcgcaacttc tccgtcatcg aggctggagg tcggctcgtc
      541 gaggatcagc acgcgtgcgt cgaccgacaa cgctctggcg atcgctacca tctgctgcac
      601 ggcgatcgga taggcgtcga gcgagcgggt gacgtcgagt gtcacgtcga gacgaagaag
      661 cgcggcctgc gcgcggcgct tgatgtcggg ccaatcgatc gcgccaaaac gctttggctg
      721 ccggccggca aagatgttct ccgccaccga gaggttcggg cacaggttca cctcctgata
      781 cagcgtgcgc acgcccgccg cttccgcctc ctgcggcgaa gcgaacgcca cgatctcgcc
      841 gcccaggcga atcgtgccgg cgtcgggcgc gactacgccg gtcagcacgt tgatcagggt
      901 ggatttgccg gcgccgttct gacccatcag cgtgtggatt tcaccgggaa acagacggaa
      961 gtcgactcgc tgcagcgctt tcacacccgg gaacgtcttg ctgacaccgg cggtggccag
     1021 aatgggctcg agtgcactcg aggcagcgcc ggcgcgagcg tcgcctgtga tggcgtcacg
     1081 ggtatgagtg ccggcggccg gcggattcgg ttcagaagcg ggatgcgtca tagacctcgc
     1141 tcaatcggcg gtgcgttggt cgggttggta caggcaggcc agattgggcg cgacctgatt
     1201 cagcggaaaa gagaccgccc gctgccgaag cagccgggcg gtcaggcacc actggagaaa
     1261 cccctgcgat ctgtccgaac tgcttgtgtc ctgcgaccat tgtatggata gctaatcagt
     1321 tagccaccag actcggcgtc aagaccgtcc gcctcctcag tacttgcgcg tcggcagagt
     1381 ctgcgccgcg acgctcatcg ggaagacagt ctcttccgtc aggatacgct tgggcaacgg
     1441 cttgccggcc accacgtcct tgaccgccga catcagctgc ggccccagca acgggctgca
     1501 ttccacgtcg acgttcatct tgcccgcggc catcgcctgg aagccgccct tggtcgcgtc
     1561 gaacgagacg acgacgatgt ccttgcccgg atgcatgccg gcctcttcca tcgcctggat
     1621 ggcgccgagc gccatgtcgt cgttatgcgc ataaactaca tttattttat ttccataagt
     1681 ttttataaac gcttccatca cctgcttgcc gccggcaagc gtgaagtcgc cgctttgtga
     1741 cgcaatgatc ttgaatttgg gatcgctttt gattacttca atcaggccgg catgccggtc
     1801 gttggcaggt gccgagccga ccgttccctg aagttccgcg atgttgacgg gacctggctc
     1861 atttttgtag tgatctgcga gccaatgacc accgcgccgg ccctcttcca agaagtccga
     1921 gccgatcatc gtcacgtaca aagacgtgtc tttcacatcg atgttgcggt cggtcaggat
     1981 caccggaatc tttgcggctt tagcttcgcg cagtaccggc tcccagccgg attcgacaac
     2041 cggagagaat gcgatcacat cgactttctg cgcgatgtag gagcggatcg ccttgatctg
     2101 gttttcctgc ttttgctggc cgtcggagaa tttgagtttg atcttcgcat ccgcggccgc
     2161 tgatttcacc gactcggtgt tggcggttcg ccatgcgctt tcggcgccga cctgcgcgaa
     2221 gccgagggtg atctgtttgt cctgcgcgta agcacccggc gttgtcagcg ccagagcgcc
     2281 gatgctcgag caaagcgcga gcgcgcccaa cgcacgacat gcggcgcgtc gtgtattcgc
     2341 catgactgtc tcctgatcgt tgttgtgtag gcgatccatc gaatccgccc agcgattgcg
     2401 acccttacgg tgcgtggatc gtgatgtagc gtagtgtcta gggcgttaac cgtccaatca
     2461 tatgattcgc gccggccata ccaatttcgg tatcgcgggt ggtatcggct gtgccgtggg
     2521 cgtaggcgat gcagcggagt tcgcctgggc gcgaccgacg atggagcgcg acgttgggcg
     2581 tgcttcttat gtgcttacgt gctcggcgtg gcttcgcgcg gcgtcaccca tcgaaacgtc
     2641 agattgattc gctctccggc cacccgcggc tccttcggca cgcgatgccg ccagtcggcc
     2701 tgcgtgtcac ccttcatcac cagcaagctg ccgcccttca gcgaaaacga ctggacgacg
     2761 ccggtcttgt tgtgccgcaa atcgaatgta cgcgccacgc ccaggctgat tgacgcaatc
     2821 accggccgag cgccgagctc gggctcgcgg tcggcatgcc accccatgct gtcggtgcca
     2881 ctgcgatagc ggttgatcag cacgctattg aagcgcgcgt ggcaagctgc ctcggcagcg
     2941 gcccgcagtt cggcgacggt cggggtccac gattgcggta agttgcgaat tccggagtag
     3001 acgtagaccg cgtccggctc gccctgccag gccgtcagcc gtggcagcgg cacacggccg
     3061 gcaggcgtgc ccatcatgtc ctgacgccat tcgacttcgt cgatgagttg cgttaaagca
     3121 tgcgccgcgg ccgcgggcgc gagccactcg ggataccagt ccacgtcggg cgccggaagg
     3181 tcatcgaaaa ggtccgccat gagattgcct gaatgcgatt gcgcacgcga tacatgcgcg
     3241 agtgagaacc ttggctatta tggcggcaac gacgatgtcg ctcacgcccg tggtttgcgc
     3301 tttcatcctg ttctctcact ctgctacttc aaaggagccc caccatgatt ctgtccgatc
     3361 aggcgctcga tcagctgttt cgcgaagcgc gcacacataa cggctggcag gccaaaccgg
     3421 tcgacgacgc cttgctcagg caactcaccc agctcgtgct gctcgggccg acctcggcca
     3481 attcgagccc cggccgcttc gtctacgtga aaacgcccga aggtaaagag aagctgcgtc
     3541 cggccctgtc ggcgggtaat ctcgagaaaa cgatggcggc gcccatcacg gtgatcgtcg
     3601 ggatggacat ggcgttttac gagcatctgc cgaaactgtt cccgcatgcc gacgcgcgta
     3661 gctggttcgc cggtaacgac aaggcgatcg cggatacggc ttttcgcaat tcgacgctgc
     3721 aaggcggcta tctgattctc gcagcccgcg cgctcggtct ggacaccggc gcgatgtcgg
     3781 gcttcgacgc cgccaaggtc gacgagacct tcttcgccgg cacgagcgtg aggtccaact
     3841 tcctgatcaa tctgggctac ggtgacccgt cgaagctatt cccgcgcagc ccgcggctct
     3901 ccttcgacga agcggcccag atcgtctgac aggccaatgg ccaccggtgc gtttacctga
     3961 agagcgccac gtagaacacc accgcgccga tcaacgcacc gatgaagcag aagccttcct
     4021 cggcctcatc gccattggaa cgcaaccacg cgccgacgac gccgaacagc cctgcgatgc
     4081 ccagcgcaac gcataccatc actacgtaaa ataacgcgct tttgcccagc tcggagaagt
     4141 cgatgtcgcg cacgccgaag tacaaggcga gcgccatcag cgatatgccc accagcggca
     4201 gcatcacgtg gctaccctct cgtccgacgt gatgtgcgtg atgcgcgtga tgtccgtggc
     4261 ggacgtgatt gcctgatcca agcagtttca tgatgcgtct cctgcctcgc cctctaccgg
     4321 cgacaaacga accatcgcgg ggccgcgacg acccttagac aaaccggttc tatttgagaa
     4381 tagtccacgc ggaacacaat tcaaagccca tttaattagc atgtcacggc cattcagccg
     4441 ggctgcggga cgggcgaatt gctgcaatgc gatacccgcc tgccgcgtca aacctgcacg
     4501 tgctcggcaa cccggtgtga gccaatttgg tgccgtcggc acggggttgc acatctcggt
     4561 agaatggtcc ggctcgtccg ctattccaag gttgctgttt gcgagccgcc gccgtctttt
     4621 cactcgagcc ggccccttcc cgcctct
//
LOCUS       DATASH010000332         6478 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3013692,
            whole genome shotgun sequence.
ACCESSION   DATASH010000332 DATASH010000000
VERSION     DATASH010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6478
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3013692"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="VHV99_09225"
     CDS             <1..629
                     /locus_tag="VHV99_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3635655.1"
                     /translation="VLEKTAQQAVRAGMIIKRIREFVKRSEPKRQATRVADIVADAVG
                     LAEIEARKRRIRIVTDLRSRLPVIYVDPVLIEQVLVNLLKNAAEAMAEARPNAVDPVI
                     RVVVRLESGFVCISVVDQGPGVDEATAERLFEPFYSTKSDGMGMGLNICRSIIESHRG
                     RLWVVNNVESDGHITGATFHCSLPIGEPDGPSNGGAQAPTPQTVTGEL"
     gene            626..1270
                     /gene="fixJ"
                     /locus_tag="VHV99_09230"
     CDS             626..1270
                     /gene="fixJ"
                     /locus_tag="VHV99_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen response regulator transcription factor
                     FixJ"
                     /protein_id="HEX3635656.1"
                     /translation="MNSPVTTQETVFVVDDDEAVRDSLRWLLEANGYRVQCFSSAEQF
                     IDAWQPHQHPGQIACLILDVRMSGMSGLELQERLIADNASLPIIFVTGHGDVPMAVST
                     MKKGAMDFIEKPFDEAELRKLVERMLDKARSESTSVQQQRAAAERLGKLTAREHQVLE
                     RIIAGRLNKQIADDLGISIKTVEAHRANIMEKLNVNTVADLLRLALSNKPQPAQ"
     gene            1832..3229
                     /locus_tag="VHV99_09235"
     CDS             1832..3229
                     /locus_tag="VHV99_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013341411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HEX3635657.1"
                     /translation="MKRSGTHHSRVLLVCAALAGAPLFTGLVTPGVVHAQSAATMSNQ
                     QLDSLTAPIALYPDALLAQVLMAATFPQDVQAAAAWSNANSKLQGDDAVKAVASEPWD
                     PSVQSLVAFPQVLATMASKPDWVSQLGTAFLGQPNDVMDSVQRLRKQAQAAGNLNTSS
                     QQKVVVEQSTIQIVPANPQVVYVPTYNPTVVYGTWPYPAYPPVYVPPPPGYAIATGLA
                     AGLAFGAGVAVANSLWGGFNWNTHDVNINVNRYNNINVNNRLNVNSNTTNWNRNANVN
                     RNVNNANLNRNVNNNLGSAQRDAFRGRDDAARAQAQQTLQNRTGQNLSGSASQRVQGI
                     HQGGTPSGNLQNRAQNVNRDNALRGAGDGNAARQNEQRGEASRNAAANRSGGFGANGG
                     GQQRAGGGFGANGGGQQRTGGDPGANGGGQQHPGGGLGANGGGVQRGGGGNFGGGGGR
                     LGGGGGGERHPGRNR"
     gene            3250..4191
                     /locus_tag="VHV99_09240"
     CDS             3250..4191
                     /locus_tag="VHV99_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2950 domain-containing protein"
                     /protein_id="HEX3635658.1"
                     /translation="MIRLFPRGALRAHVLTFAAILGTTGTLLVAPVLLLGTTAAHAQA
                     VYPSADAAANAFVDALARNDEGALKHVLGNDFHRFIPAEGIGEDDIYQFLGAWSKGHQ
                     IVQDPVKSKGRATAHLAVGDSGWTLPIPLVQAAHGWRFDPPAAQDEMLTRRIGRNEEA
                     AIQTSLAYLDAQNDYHNLTQHYARRFVSTQGQHDGLYWATAPGEAESPLGPLAATMPN
                     GTLPKEAYHGYHYRILTAQGPHAKGGAQNYVENGVLTKGVGLIASPAEYGKTGVMSFI
                     VNRDGQVYQKNLGPQTARAAAAIKSFDPDDSWQPVQQ"
     gene            4792..5652
                     /gene="folD"
                     /locus_tag="VHV99_09245"
     CDS             4792..5652
                     /gene="folD"
                     /locus_tag="VHV99_09245"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEX3635659.1"
                     /translation="MTAKLIDGLALSKTLRADVATRAAALTARGHQPGLAVVLVGDNP
                     ASEVYVRNKVKACHDNGLGSSFDRYPADLPEADLLARIDELNRDPRIHGILVQLPLPP
                     HIDSHKVIEAIAPEKDVDGFHVANAGALMTGRPLFRPCTPYGVMKMLAAYEIPLQGAN
                     AVVIGRSNIVGKPMALLLLEAGATVTICHSKTRDLAAHTRNADVVVAATGLRNILTAD
                     MVKPGAAVIDVGMNRDEAGKLCGDVDFAGVKEVAGYITPVPGGVGPMTITMLLVNTIE
                     AAEREAAANA"
     gene            complement(5883..>6478)
                     /locus_tag="VHV99_09250"
     CDS             complement(5883..>6478)
                     /locus_tag="VHV99_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX3635660.1"
                     /translation="GMLGLLSSARGVALAVVLGCAAFVFRVHLPHLQIGLRDFSETPG
                     GYSFWPEPFFLLGMLLYGWRERVDINGLAALALAMVFLVFRDTAGAQPLFYLAFVYGV
                     LWVGTTPLLRRFVPRHDYSYGIYLYGFMVQQCVANIAPHLNHVAAVLIAAPFILLCAA
                     LSWHFVERPVLMWCRRRLARSQTPLGAGMAAGDQAVR"
BASE COUNT         1265 a         2224 c         1972 g         1017 t
ORIGIN      
        1 cggtgctcga aaaaaccgcg caacaggcgg tgcgcgccgg catgatcatc aagcgcattc
       61 gcgagtttgt gaagcgcagc gagcctaagc ggcaagccac gcgggtggcc gatatcgtcg
      121 ccgacgccgt gggtctggcc gagatcgagg cgaggaagcg ccgcattcgc atcgtcacgg
      181 atctgcgctc gcgcctgccg gtgatctacg tcgacccggt gctgatcgag caggtgctgg
      241 tcaatctgct gaagaacgcc gccgaagcca tggccgaggc ccgtccgaat gcggtcgatc
      301 cggtgatccg cgtggtcgtg cggctggaga gcgggtttgt ctgcatcagc gtcgtcgacc
      361 aggggccggg cgtcgacgaa gcgacagccg agcggctgtt cgaaccgttt tacagcacca
      421 agtccgatgg catgggcatg gggctgaaca tttgccgttc gattatcgaa tcgcaccgcg
      481 gccgtctgtg ggtggtcaac aacgtcgaat ctgacggcca catcacaggc gccacgttcc
      541 attgcagtct gcctattgga gagcctgatg gcccgagcaa cggcggggca caggcgccga
      601 caccacaaac cgttacggga gagctatgaa cagcccagtc accacacagg aaactgtctt
      661 tgtcgtcgac gacgacgagg ccgtgcgaga ttcgctgcgc tggctgctgg aggcgaacgg
      721 ctaccgcgtg caatgcttct ccagcgccga gcagttcatc gacgcatggc agccgcatca
      781 gcatccgggc cagatcgcgt gcctgattct ggatgtgcgg atgtccggca tgagcgggct
      841 cgagttgcag gaacgcctga tcgccgacaa cgcatcgctg ccgatcatct tcgtgaccgg
      901 tcacggcgac gtgccgatgg ccgtgtccac gatgaaaaaa ggcgcgatgg acttcatcga
      961 aaagccgttc gacgaagccg agttgcgcaa gctggtcgag cgcatgctcg acaaggcgcg
     1021 cagcgaaagc accagcgtgc agcagcagcg cgccgcggcg gagcgtctcg gcaagctgac
     1081 cgcgcgtgag catcaggtgc tcgagcgcat catcgccggc cgcctgaaca agcagatcgc
     1141 cgacgacctc ggcatcagca tcaagacggt cgaagcgcac cgcgcgaaca tcatggaaaa
     1201 gctcaacgtc aacacggtcg ccgatctgct gcgactggcg ctgtcgaaca agccgcaacc
     1261 ggcgcaataa gcataacggc cgcggcgccg cccgcgggtc gccactgcgc ttgaacggcc
     1321 ggactgtgga aaggtccggc ctgttttatc tttgtctttt ccttgccctt aacctttccc
     1381 cgcttcgcga caacaagccg cggcgtggcc gatgtatcgg cgtcggtatt cgcacttcgg
     1441 tccgcattgg cgtccccatc cgtcaatcat tacgcattac tcagcatgcg catacgtcgc
     1501 agggcaataa gctctttcct ccgacaagat tgacaagctt cgccgtgccg cttaatttac
     1561 gcagaactcc tcagcaggga ccgtcgatgc ccgatgaacg caggacaagc gtgctgccgt
     1621 tgtcgtatgc ggcgttgcca tgcgttgcga agcagtcaag gcgcttcggc cgcgccgctg
     1681 aggtacctgc cttatcgaac ggctctcaca cgaaaccgag ccctgccttt ctgccttcac
     1741 tcgcttccat cgccattcca gcgcgcccgg gcaacgcacc atccgttgtg cattgacttc
     1801 gcgcgtctaa cgcagtcggg aggacttcat cgtgaaacga tccggaacac accattcgcg
     1861 tgtgttgctt gtatgcgccg cccttgcagg cgcgccactc ttcaccggtc tggtcacgcc
     1921 gggcgtcgtc catgcgcaaa gcgccgcgac gatgtcgaac cagcaactcg attcgctgac
     1981 cgcacccatc gcgctctatc ccgacgcact gctcgcgcag gtgctgatgg ccgccacttt
     2041 cccccaggac gtgcaggccg cagccgcatg gtcgaacgcg aactcgaagc tgcaaggcga
     2101 cgacgccgtc aaggcggtcg catccgagcc gtgggatccg agcgtgcaat cgctggtcgc
     2161 ctttccgcag gtcctcgcga cgatggcgtc gaaaccggac tgggtctcgc aactcggcac
     2221 cgcctttctc ggccagccga atgacgtgat ggactccgtg caacgcttgc gcaaacaggc
     2281 gcaagccgcc ggcaatctga acacgagttc gcagcagaaa gtcgtggtcg agcaaagcac
     2341 gatccagatc gtgccggcca atccgcaagt ggtatacgtg cccacgtata acccgactgt
     2401 cgtctacggt acatggccct atcctgccta cccgccggtg tatgtcccac cgccgcccgg
     2461 ttatgccatc gcgaccggac tcgccgccgg gcttgcgttc ggtgccggcg tcgccgtggc
     2521 caattcgctg tggggcggct tcaactggaa cacgcatgac gtcaacatca acgtgaaccg
     2581 gtacaacaac atcaatgtga acaaccggct caacgtgaac agcaacacca cgaactggaa
     2641 ccgtaacgcc aacgtcaatc gcaacgtcaa caacgcgaat ctcaaccgca acgtcaacaa
     2701 caacctcggg agcgcccagc gcgatgcctt ccgcggccgt gacgacgcgg cccgtgccca
     2761 ggcgcaacag accttgcaga accgtaccgg ccagaacctc agcggcagtg ccagccagcg
     2821 cgtgcagggg attcatcagg gcggcacccc gagcggcaac ctgcagaacc gcgcgcagaa
     2881 cgtcaatcgc gataacgcgt tgcgcggcgc cggcgacggc aacgccgcgc ggcagaacga
     2941 gcagcgtggc gaagcgagcc gcaacgcggc cgcgaaccgt tccggcggct ttggtgccaa
     3001 cggtggcggc cagcaacgcg cgggcggcgg tttcggcgcg aacggcgggg gccagcaacg
     3061 tacgggtggc gatcccggtg cgaacggcgg tggccagcaa cacccgggcg gcggtctcgg
     3121 cgcgaacggc gggggtgtcc agcgcggcgg tggcggcaac ttcggcggtg gcggcggcag
     3181 actgggcggt ggcggcggcg gtgagcgcca tcccggccgc aaccgttgaa cgatcaagag
     3241 gagtctctga tgattcgctt attcccacgc ggcgccttgc gcgcccacgt tctgacattc
     3301 gccgccatcc tcggcacgac cggcaccttg ctggtcgcgc cggtactgtt gctcggcacg
     3361 accgccgcac acgcgcaggc ggtctatccc agcgccgacg ccgccgccaa tgcgttcgtc
     3421 gacgccttgg cccgcaatga cgaaggcgcg ctcaaacacg tgctcggcaa cgattttcat
     3481 cgcttcattc ctgctgaagg aatcggcgag gacgacatct accagttcct cggcgcatgg
     3541 tcgaaaggac atcagatcgt ccaggatccg gtgaagagca agggacgcgc gaccgcgcat
     3601 ctggctgtcg gcgacagtgg ctggaccttg ccgattccgc tcgtgcaggc cgcgcacggc
     3661 tggcgcttcg atccgcccgc cgcacaggac gaaatgctga cgcgccgcat cggccgcaac
     3721 gaagaggcag cgatccagac ttcgctcgcc tacctcgacg cgcaaaacga ttatcacaac
     3781 ctgacgcagc actacgcgcg gcgcttcgtc agtacgcaag gccagcatga cggcctgtac
     3841 tgggcgacgg cgccgggcga agcggagagc ccgctcggac cgctcgccgc caccatgccc
     3901 aacggcaccc tgccgaaaga ggcctatcac ggctaccact accggatcct gacggcgcaa
     3961 ggtccgcatg cgaagggcgg cgcgcagaac tacgttgaga acggtgtgct gaccaaaggg
     4021 gtcggtctga tcgcctcgcc ggcggaatat ggcaagactg gggtgatgag ctttatcgtt
     4081 aatcgggacg gtcaggtcta tcagaagaac ctcgggccgc agaccgcgcg cgcagcggcc
     4141 gccattaaat cgttcgaccc ggacgatagc tggcagccgg tgcaacagta gcgtccgctc
     4201 gatggccgcg gcggcgcata agccgtccgc ggccgatcca cgctgagatc cagcgccggt
     4261 atcgacgccg gcgcgaggcc agtctccagc gccgcaatac gtgcgggcgt ccatgccagg
     4321 ccgcaccgcg actagctacg tccaggcccg ccaccgcccc aacgccggca tgcatgttga
     4381 cgtccgtgct ggcacgcacc tccgccgatc acatctagcc cggcccgagc acttggcgct
     4441 ggcatcccct ctgatacgcc tggccaccga cgacgctagc gcctcaacat cactcagcac
     4501 aggcatccgt gccgacctcc acgctgacac gcacggacac caatcacgtc tagccgcctg
     4561 caaccctcag ccctcagcgc cggcccgcat cgcgagcgcc ccgcgccacc cggaatcctg
     4621 acatggcacg ccctgcgggg ccggtataat gtcggacttt gcagcgctgc ttgtccctga
     4681 tccgcgccgc gcgcttttca cgtgccggtc cgtttccgat gaaaagcgcc atcgcacgtg
     4741 ccgcgcgcct tgcgcctcct ccgctttcca atctgcccat ctcgaccgac catgactgcc
     4801 aaactgatcg acggcctagc cctttccaag accctgcgcg ccgacgtcgc cacgcgtgcc
     4861 gccgccctca ccgctcgcgg ccatcagccg ggcctcgccg tggtgctggt cggcgacaat
     4921 ccggccagcg aagtctatgt gcgcaacaag gtcaaggcgt gccacgacaa cggactcggt
     4981 tcctcgttcg accgctatcc ggccgatctg ccggaagccg atctgctggc gcgcatcgac
     5041 gaactgaacc gcgacccgcg catccacggc attctggtgc aactgccgct gccgccgcat
     5101 atcgacagcc acaaggtgat cgaagccatc gcgccggaaa aggatgtcga cggttttcac
     5161 gtcgccaacg ccggtgcact gatgaccggc cggcccctgt tccgcccgtg cacgccgtac
     5221 ggcgtgatga agatgctggc tgcgtatgaa attccgctgc aaggcgcgaa tgcagtggtg
     5281 atcggccgct cgaacattgt cggcaagccg atggcgctgc tgttgctcga agcaggcgcg
     5341 accgtcacga tctgccatag caagacgcgc gacctcgccg cccacacgcg taatgccgac
     5401 gtggtggtcg ccgccaccgg tctgcgcaac attctcacgg cggacatggt gaagcctgga
     5461 gccgccgtga tcgacgtcgg catgaatcgc gacgaagccg gcaagctgtg cggtgacgtc
     5521 gatttcgcgg gcgtcaagga agtggccggc tatatcacgc cggtgccggg cggcgtcggt
     5581 ccgatgacca tcaccatgct gctcgtcaac acgatcgaag ccgccgagcg cgaagcggcg
     5641 gcaaacgcgt aaggctgaag ggcacaacca tgccggcgaa ccttttcgcc ggcgtgcaag
     5701 gctgcggttg ccgcaccgag ttcgagaccg catcccgcca gcaacccctg gcgtggattc
     5761 cctgctctca ttcctgcatc gtgcaccggc ctcgatacca ggcgtcaatc ctcgccggcg
     5821 ctatctgtta gcggttcact gttccatgcc aggcgccaaa ccaccttgcg ctaaccagcc
     5881 gctcagcgca ccgcctgatc cccagccgcc atccctgcgc cgagcggcgt ttgcgaccgc
     5941 gccagacgcc gccggcacca catcagcacc ggccgctcca caaagtgcca cgacagcgcc
     6001 gcgcacagca gaataaacgg cgcggcgatc agcactgcgg ccacatggtt caagtgcggc
     6061 gcgatattgg cgacgcattg ctgcaccata aagccgtaca gataaatccc atacgagtaa
     6121 tcgtgacgcg gcacgaaacg ccgcaacagc ggcgtcgtgc ccacccacag cacgccgtag
     6181 acgaaagcca gatagaacag cggctgagcg ccggccgtgt cgcggaacac cagaaatacc
     6241 atcgccagcg caagcgccgc caagccgttg atgtcgaccc gctcgcgcca tccgtacagc
     6301 agcatcccca gcaagaaaaa cggctccggc cagaacgaat agccgccggg cgtttcgctg
     6361 aagtcacgca gcccgatctg cagatgcggc agatgcacgc ggaaaacgaa cgcagcgcat
     6421 ccgagcacga ccgccagcgc cacgccgcgc gcactggaga gcagccccag catgccgg
//
LOCUS       DATASH010000333         2592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4003554,
            whole genome shotgun sequence.
ACCESSION   DATASH010000333 DATASH010000000
VERSION     DATASH010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4003554"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(248..1132)
                     /locus_tag="VHV99_09255"
     CDS             complement(248..1132)
                     /locus_tag="VHV99_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3635661.1"
                     /translation="MRFDLIDLRLFLHILETGSITHGAARANMSLPSASARLRGMEEA
                     LGEPLLERGRRGVESTSTGDTLAHHARLVLGQIDRMQGELGEHQAGRKACIRLWTNTA
                     AVTEFLPSAVGLFLRDHPNVQIDLKERQSTETVKAVLTGAAEIGIISDAVEHGALQTL
                     PFAVDKLVLVTSRDDPLAARRRVTLAEVADREFIGLSTGSALHAYLGERALLSGQALR
                     IRAHMRTFENVCCMVEQGAGIAIVPATAAKRYRGTQGIRTIELTDTWATRQLLICMRD
                     LNALKRPEKALVRHLAGI"
     gene            1287..2039
                     /locus_tag="VHV99_09260"
     CDS             1287..2039
                     /locus_tag="VHV99_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX3635662.1"
                     /translation="MISSFARRVSVVAATFVVAGFVKGVTGMGLPTVAMGVLGTLMLP
                     AQAAAMLLLPSFVTNVWQLFAGPGVRMLVKRLWLMMAGIVAGTVGAASFIAGGGGAWA
                     VVGLGAALTLYAAAGLMAWRFSVPPRHERWLSPAVGAVTGVITGGTGVFVVPAVPYLQ
                     GLALKKEDLVQALGLSFTVSTVALAIGLAREHAFAVDDLGESLFAVVPALIGMWIGQR
                     VRGRISPQTFRRWFFICLLGLGIQLLARAFVS"
     gene            2411..>2592
                     /locus_tag="VHV99_09265"
     CDS             2411..>2592
                     /locus_tag="VHV99_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006476236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3635663.1"
                     /translation="MSKLTGKVAVVTGASKGIGAAIAKALAAEGASVVVNYASSKAGA
                     DDVVAAITAAGGKAVAV"
BASE COUNT          427 a          769 c          862 g          534 t
ORIGIN      
        1 acgctgaacg agcgcacgcc gtgtgcttcg tcgcgtacgt tgtcgacgcg cacattgaag
       61 gtggttcttt ccatgatctt gccggcaaaa ggacagagag gagcagagcg gacgccgcgt
      121 gcattgcgcg gcgtccggtt gtgcgtcaag gataagaaac ggccgacaat caggcaaatc
      181 gattaaaaat cagttgtgga ataaatcaca ctgataacag tgcgccaggc gtgcacgctc
      241 gccgccctca gatacccgcc agatgccgga ccagggcctt ttccggccgc ttcagcgcgt
      301 tcaggtcacg catgcagatc agcaactggc gcgtggccca ggtgtccgtc aattcgatcg
      361 tgcgaatgcc ctgtgtgccg cgatagcgtt tcgcggcggt ggccggcacg atcgcgatgc
      421 ccgcgccctg ttcgaccatg cagcagacgt tttcgaaggt tcgcatgtgc gcgcgaatcc
      481 tgagcgcctg tccggatagc agtgcgcgtt cgccaagata ggcgtgcagc gcgctgcccg
      541 tactcaggcc aatgaattca cgatcggcca cttccgcgag cgttacgcgg cgccgcgcgg
      601 ccagcgggtc gtcacggctc gtgaccagca cgagtttgtc gacggcgaag ggcagcgtct
      661 gcaacgcgcc atgttcgacg gcatccgaga tgatgccgat ctccgccgcg ccggtcagca
      721 ccgctttcac ggtttcggtg ctttgccgtt ccttcagatc gatctgcaca ttggggtggt
      781 cgcgcaaaaa caggccgacc gctgacggca agaactccgt gaccgcggcg gtattggtcc
      841 acaagcggat gcacgctttg cgcccggcct gatgttcgcc gagttcgcct tgcatgcggt
      901 cgatctggcc gagcacgagt ctcgcgtgat gcgcgagcgt atcgcctgtc gacgtggact
      961 ccacgccgcg ccggccccgc tccagcaacg gctcgcccaa ggcttcctcc atgccgcgca
     1021 aacgcgcgct agccgacggc agcgacatgt tcgcgcgcgc cgcgccatgc gtgatgctgc
     1081 cggtttcgag aatgtgaagg aaaaggcgga ggtcgatcag atcgaagcgc atggagtcgg
     1141 atgtgagttg ggaccgcaat gggggctgca atcgcggctg agttcgagcc ttaggcaaag
     1201 cctaaggcac agtacgtatc ttacgcattg tggacggcgc cgcggcaatc gaaaatcgct
     1261 ggcatgacat ccagaacctc atcccgatga tctcttcgtt tgctaggcgc gtgagcgtgg
     1321 ttgcggccac ctttgtagta gccggcttcg tcaagggcgt gaccggtatg ggcttgccga
     1381 cggttgcgat gggcgtactc ggcacgttga tgctgccggc gcaagcggcc gcgatgctgt
     1441 tgttgccgtc gttcgtgacc aacgtctggc agctgttcgc gggacccggc gtgcgaatgc
     1501 tggtcaagcg cttgtggctg atgatggcgg gcatcgttgc gggcacggtg ggcgctgcgt
     1561 cgttcattgc cggcgggggc ggcgcatggg ccgtcgtcgg tctgggtgcc gcgctgacgc
     1621 tctatgccgc ggccggcctg atggcttggc gcttctcggt gccgccgcgt cacgaacgct
     1681 ggctctcgcc ggcggtcggc gcggtcaccg gtgtgatcac cggtggtaca ggcgtatttg
     1741 tcgtgcccgc ggtgccttat ctgcaagggc tcgcgttaaa gaaagaggac ctggttcagg
     1801 cgctcggcct gtccttcacc gtttccaccg tcgcgctcgc gatcggattg gcgcgcgagc
     1861 atgcgtttgc cgtcgacgat ctgggcgaat cgctgttcgc cgtggtgccc gccctgatcg
     1921 gcatgtggat cggacaacgg gtgcgcgggc gcattagtcc gcagactttc cggcgctggt
     1981 tctttatctg cctgcttggc ctcggcattc agttgctggc gcgtgcgttc gtttcataga
     2041 gaggtagctt ggagagtcaa ggagggtagg atcatgtggt cggctattct ggatacgctg
     2101 ggtgcgttgg cggtgctggc aagcgtgacg gtgcagatcg cgctgatgcg caaacgctga
     2161 caccacccgg tggtttgcga catcccgcac gcatttgtct gatcttcgcg cgcccttgcg
     2221 cacctcgcgc tttccgattg ttcggctcat ccgacgtatc tattcgcctt cagccgcttt
     2281 atccttacgc ccggcgtgcc tacattgtgt ccagtgccag cgccacggca tttccgcttt
     2341 catcgacgtc agcctggcga atctttcgca agcgcgacgt tggcgcgaac tccatccaaa
     2401 ggaaacgatc atgagcaagc tcacaggtaa ggttgcggtt gtaacaggcg catcgaaagg
     2461 catcggtgcc gcgattgcga aggccttggc agcagagggt gcgtcagtgg tggtgaacta
     2521 tgcgtccagc aaggcgggcg cggacgatgt ggtcgccgcg atcaccgcgg cgggcggcaa
     2581 ggcggtggcc gt
//
LOCUS       DATASH010000334         4341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_442337,
            whole genome shotgun sequence.
ACCESSION   DATASH010000334 DATASH010000000
VERSION     DATASH010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442337"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1476
                     /locus_tag="VHV99_09270"
     CDS             <1..1476
                     /locus_tag="VHV99_09270"
                     /inference="COORDINATES: protein motif:HMM:NF012592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HEX3635664.1"
                     /translation="LGEAAEFMFDHNIFQENLVGVDFCEKMVAETNPGVLAQAERTEA
                     PHADAHGYRTIADIMRALNPFSGEFYREALHVSRTTREMFCLMEGRHVHPSTLYPGGV
                     GTVPTIQLFTDYITRLMKYVEFMKKVVPLHDDLFDFFYEALPGYEEVGRRRVLLGCWG
                     SFQDPNVCDYNYNTMTQWGRGMFVTPGVVVDGELVTTDLVDINLNIRILLGSSYYDDW
                     DHEETFVKNDPLGNPVDRKHPWNQTTLPHPQKRKFDGNYTWVMSPRWLDKRTGDHLAL
                     DTGGGPIARLWATALAGLVDIGYIKATGHSVKIYLPKTALKPEVEFEWKIPKWSNALE
                     RDRARTYFQAYSAAAALYFAEQALAELHAGRTRTFSDFTVPEEGIGCGFHEAVRGVLS
                     HHLVIREGKIANYHPYPPTPWNANPRDIYGTPGPYEDAVQNTPIFEENGPDKFKGIDI
                     MRAVRSFDPCLPCGVHMYVGNGKTIDTSHSPTFGVMQGAHT"
     gene            1473..2072
                     /locus_tag="VHV99_09275"
     CDS             1473..2072
                     /locus_tag="VHV99_09275"
                     /inference="COORDINATES: protein motif:HMM:NF013286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEX3635665.1"
                     /translation="MSAPANDQRAVAAQQHRIEELIAALEALDDPPARESAQELLQVV
                     LDLHTNGLARLLEIVMSAGAMDGALVEAFERDAGVSAMLLLHGLHPQDLPTRVSRAVE
                     KMRPLLGVQGIQIELLDAAEEAVRVKVAGRLQGKHNSVGELRQEIELAIFEAAPEAMR
                     VDIAGLQDVNVHELRFVPNGRTEHTEHTEVASRTEAGAQ"
     gene            2069..2707
                     /locus_tag="VHV99_09280"
     CDS             2069..2707
                     /locus_tag="VHV99_09280"
                     /inference="COORDINATES: protein motif:HMM:NF040271.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5947 family protein"
                     /protein_id="HEX3635666.1"
                     /translation="MTPATATTRGWVAGVRHFVRKANDTHCELCGALLPADHPHLFEI
                     DKRQLYCCCRACALLFGSQQNARYRPVPRDARYLDGFRMSDAQWDALAIPIEIAFFFH
                     DSSAQRIVALYPGPAGGTQSLLDLQAWTELVADNAWLAQLEPDVEALLVNRSEGAREY
                     YRVPIDQCFALTGAIRARWRGVSGGRQVWDTIHGFFGALKSQGRVPEGLLHA"
     gene            2700..3359
                     /locus_tag="VHV99_09285"
     CDS             2700..3359
                     /locus_tag="VHV99_09285"
                     /inference="COORDINATES: protein motif:HMM:NF039986.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6084 family protein"
                     /protein_id="HEX3635667.1"
                     /translation="MPDLGFTVESADVVPFAAAPLLMFKLHIVDTAADRSAHREIASI
                     ALQCQIQIEAAKRRYAPAEQYALEDLFGIPTRWGDTLRTMLWTHASVVAPPFTGECTL
                     DLPVPCSYDFNVAATRYFYGLEEGEIPLMLQFSGTVFYREADGALQAAPIPWHKEAAY
                     RLPVALWRDMMARYYPNGAWLCLHRDVFDRFSRFKTRGGLTSWEQALTSLLDKLEEDI
                     S"
     gene            3356..>4341
                     /locus_tag="VHV99_09290"
     CDS             3356..>4341
                     /locus_tag="VHV99_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635668.1"
                     /translation="MNAVESVVQAVLYEGYMLYPYRPTSVKNRQRWTFGGVYPRAYAE
                     ASGSDPWVTQSECLLRGSERTRVAVRPGFLQVIERMVLDVAENAGVRPESGDPAWRTV
                     SLLDIDERRYTPWQEAAERHLALPVLVLGELLNAPRGIPFSFDAGSELEPLADRHGAL
                     RGALRRMRAALQGRVEVSAASVAAGVYRLKVRIVNETPLDQARSLTRDAASLYALVSC
                     HTVLTVERGTWVSSIDPPDDIAAASAACRNIGVWPVLVGDEQQPDTMLASPIILYDFP
                     QIAPESAGDLFDATEIDEILSLRILTMTDAEKRDMAAVDPRARALLERTEALG"
BASE COUNT          774 a         1333 c         1445 g          789 t
ORIGIN      
        1 ctcggcgagg ccgccgagtt catgttcgac cacaacatct tccaggagaa cctggtcggg
       61 gtcgacttct gcgagaagat ggtcgcggag acgaaccccg gcgtactggc gcaggccgag
      121 cgcaccgagg cgccccacgc cgacgcccac ggctaccgca cgatcgccga catcatgcgc
      181 gccctcaacc cgttctcggg cgagttctac cgcgaggcgc tccatgtcag ccgaacgacg
      241 cgcgagatgt tctgcctgat ggaagggcgc cacgtgcacc cgtcgacgct gtatcccggc
      301 ggcgtgggca ccgtgccgac cattcagctc tttaccgact acatcacgcg tttgatgaaa
      361 tacgtggaat tcatgaagaa ggtcgtgccg ctgcacgacg atctgttcga cttcttctac
      421 gaggcactgc ccggttatga ggaagtgggc cggcgccgtg ttctgctcgg ctgctggggt
      481 tcgttccagg acccgaacgt gtgcgattac aactacaaca ccatgacgca atggggccgc
      541 ggcatgttcg tcacaccggg cgtggtggtg gacggcgaac tggtcacgac cgacctcgtc
      601 gacatcaacc tgaacattcg cattctgctc ggcagctcct attacgacga ctgggatcac
      661 gaagaaacct tcgtgaagaa cgacccgctc ggcaatccgg tggatcgcaa gcatccgtgg
      721 aatcagacca cgctgccgca tccgcagaag cgcaaattcg acggcaacta tacgtgggtg
      781 atgtcgccgc gctggctcga caagcgcacg ggcgatcacc tcgcgctcga taccggcggc
      841 ggtccgattg cgcgtttgtg ggccacggcg ttggcgggac tcgtcgacat tggctatatc
      901 aaggcgaccg gtcacagtgt gaagatctat ttgccgaaga ccgcgctcaa gcctgaagtc
      961 gaattcgaat ggaaaatacc gaagtggagc aatgcgcttg aacgcgatcg cgcccgtacg
     1021 tattttcagg cgtattcggc ggcagcggca ttgtatttcg ccgagcaggc gctcgccgaa
     1081 ttgcacgcgg gccgcacccg cacgttcagc gatttcacgg tgcctgaaga aggcattggc
     1141 tgcggtttcc atgaagcggt gcgtggggtg ctgtcgcatc acctcgtgat tcgcgagggc
     1201 aagatcgcca actatcatcc gtatccgcct acgccctgga acgctaatcc gcgcgatatt
     1261 tacgggacgc ctggaccgta cgaggacgcg gtgcagaaca cgccgatctt cgaagagaac
     1321 ggccccgaca agttcaaggg catcgacatc atgcgcgcgg ttcgcagctt cgatccctgc
     1381 ctgccgtgcg gcgttcatat gtatgtgggc aacggcaaga cgatcgacac ctcgcactcg
     1441 ccgaccttcg gcgtgatgca gggagcgcat acgtgagcgc accagcaaac gaccagcgcg
     1501 cggtcgccgc ccagcaacac cgcatcgagg aactgattgc ggcgctggaa gcgctcgacg
     1561 atccgcccgc gcgcgaaagc gcccaggaac tgctgcaggt ggtgctcgat ctgcatacga
     1621 acgggctcgc gcgtctgctg gagatcgtca tgtccgccgg ggcgatggac ggtgcgttgg
     1681 tcgaggcgtt cgaacgcgac gccggtgtgt cggcgatgct gctgttgcac ggtttgcatc
     1741 cgcaagatct gccgacgcgc gtgtctcgcg cggtggagaa gatgcggccg ctgctcggcg
     1801 tgcaaggcat ccagatcgaa ttgctggacg ccgcggaaga agcggtacgc gtaaaagtgg
     1861 ccgggcgttt gcagggcaag cacaactcgg tcggcgaatt gcggcaggaa atcgaactcg
     1921 ccattttcga ggcggcgccc gaggccatgc gcgtcgacat tgccggtttg caggacgtca
     1981 acgtgcatga actgcgcttc gtgcctaatg ggcgcactga gcacactgag cacaccgagg
     2041 tagccagccg gaccgaggcc ggcgcgcaat gacacccgcc accgccacga cgcgcggctg
     2101 ggtcgcgggt gtgcgccatt tcgtgcgcaa agccaacgac acgcactgtg aattgtgcgg
     2161 ggcgctgctg ccggccgatc atcctcatct gttcgaaatc gacaaacgcc aactgtattg
     2221 ctgctgccgt gcgtgcgcat tgttgttcgg cagtcagcag aatgcgcgct atcgtccggt
     2281 gccgcgcgac gcgcgctatc tcgacggttt tcgcatgagc gacgcgcagt gggatgcact
     2341 ggcgattccc atcgagattg cttttttctt tcacgacagt tcagcgcaac ggatcgtcgc
     2401 gctgtatccc ggaccggcgg gcggcacgca atcgctgctg gatctgcagg cgtggaccga
     2461 actcgtcgcc gacaatgcgt ggctcgcgca actggagccc gacgtcgaag cgctgcttgt
     2521 gaaccgcagc gaaggcgcgc gcgagtacta ccgcgtgccg atcgatcaat gtttcgcgtt
     2581 gaccggcgcg attcgcgcgc gttggcgcgg cgtctcgggc ggacggcagg tctgggacac
     2641 gattcatggt ttcttcggcg cgctgaagag ccagggccgc gtaccggagg gtttgctcca
     2701 tgcctgatct cggattcacc gtcgagtcgg cggacgtggt gccgtttgcc gcggcgccgc
     2761 tgctgatgtt caagctgcat atcgtggaca ctgccgcgga caggtcggcg caccgcgaga
     2821 tcgccagcat cgcgctgcaa tgccagattc agatcgaggc cgcgaaacgc cgctacgcgc
     2881 ccgccgagca gtacgcgctc gaggatctgt tcggcattcc gacacgttgg ggcgatacgt
     2941 tgcgcacgat gctatggacg catgcgtcgg tggtcgcgcc accctttacc ggcgagtgca
     3001 cgctcgatct gcccgtgccg tgcagctacg attttaatgt ggcggcgacc cgatattttt
     3061 acggactcga ggagggggag attccgctga tgctgcagtt cagcggcacg gtcttctatc
     3121 gcgaggccga tggcgcgttg caggccgcgc cgattccgtg gcacaaggag gcggcgtacc
     3181 gtctgccggt ggcgctatgg cgcgacatga tggcgcgcta ttacccgaac ggcgcatggt
     3241 tatgcctgca tcgcgacgtg ttcgatcgct tctcgcgctt taagacccgc ggggggctga
     3301 cgagctggga gcaggcgctc acgagcctgc tcgacaagct cgaggaggac atatcgtgaa
     3361 cgcggttgag agcgtggtgc aggcggtgct gtacgaaggc tacatgctgt atccgtaccg
     3421 gccgacttcg gtgaaaaacc ggcaacgctg gaccttcggc ggcgtctatc cgcgtgccta
     3481 tgcggaggcg tccggcagcg atccttgggt aacgcaaagc gagtgtctgc tgcgcggcag
     3541 cgagcgcacg cgcgtggcgg tgcggcccgg tttcttgcag gtgatcgagc gcatggtgct
     3601 ggacgtagcg gagaacgccg gggtacggcc agagtcgggc gacccggcgt ggcgcacggt
     3661 gtccctgctc gatatcgacg aacggcgcta tacgccgtgg caggaagcgg ctgaacggca
     3721 tctcgcgctg cccgtgctgg tgctgggcga attgctgaac gcgccgcgag gtattccctt
     3781 ttcgttcgat gcgggcagcg aactcgaacc gctggcggat cggcacggcg cgctgcgtgg
     3841 cgcgttgcga cgtatgcgcg cggcgctgca aggccgcgtc gaagtgagtg cggcaagcgt
     3901 ggcggcgggc gtctatcggc tgaaagtgcg catcgtcaat gagacgccgc tcgatcaggc
     3961 gcgcagcctc acgcgcgacg ccgcgtcgct gtacgcgttg gtctcgtgcc acaccgtgct
     4021 gaccgtcgag cgcggcacat gggtctcgtc gatcgatccg cccgacgaca tcgcggcggc
     4081 gtcggccgcg tgccgcaata tcggcgtttg gccggtgctg gtcggcgatg agcagcagcc
     4141 cgacacgatg ctcgcctcgc cgatcatcct ctacgatttt ccgcagatcg cgcccgaaag
     4201 cgccggcgat ctgttcgacg ccaccgagat cgacgaaatc ctgtcgctcc gcatcctcac
     4261 catgaccgat gcggaaaagc gcgacatggc ggcggtcgat ccgcgcgcgc gggcgctgct
     4321 ggagcgcacc gaggcgctgg g
//
LOCUS       DATASH010000335         9469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_442350,
            whole genome shotgun sequence.
ACCESSION   DATASH010000335 DATASH010000000
VERSION     DATASH010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 9469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9469
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442350"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..1305
                     /locus_tag="VHV99_09295"
     CDS             13..1305
                     /locus_tag="VHV99_09295"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019254972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HEX3635669.1"
                     /translation="MSVPQQAFLRDAMRRMNMTRDTFASRIGVSRRALDTWLLPDDSQ
                     ESRAMPEIVERFVSEIAVHGEPGEKYTQSVDSQSLASQMLFEGKPQLLSVDQFSRDSV
                     EALFRVADIMQPIARRRKISRVLEGAVLGNLFFEASTRTRVSFGAAFCRLGGSVCDTT
                     GFTFSSMAKGESIYDTSRVMSGYVDALVIRHPEQGSVAEFARATNVPVINGGDGPGEH
                     PSQALLDLYTIQREFSRLGKIVDGAHIALVGDLKYGRTVHSLVKLLALYRGIKFTLIS
                     PPMLEMPSYIIEQISRNDHVIEQTHDLPAGLRGADVVYATRIQKERFADESFEGYTPD
                     FQINQALMDSVCGADTLIMHPLPRDSRPGANDLSVDLNHDSRLAIFRQTDNGIPVRMA
                     IFAVLLGVEKLVQHSMRDAAWRPPAYLGPDDAVFHGID"
     gene            1541..3004
                     /locus_tag="VHV99_09300"
     CDS             1541..3004
                     /locus_tag="VHV99_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX3635670.1"
                     /translation="MADTSYMARKSIADIVASAEGGERQLSKSLGALSITAMGIGAIV
                     GAGIFVLTGTAAAHYAGPSIILSFVLGGIACAFVGLCYSELAAMLPVCGSSYTYTYAT
                     LGELFAWVIGWDLILEYAMGAATVAVGWSGYIVSLLHNVGINIPPAMAAARGTTVTLA
                     DGSTVAGVVNLPAVFIIAVLTIMLVLGTKESARLNNIMVAIKLTVVLAFIVIGAFFVH
                     PANWHPFIPQNTGEFGNFGVSGILRGSAVVFFAFIGFDAVSTAAQEARKPQIDMPIGI
                     LGSLAICTVLYIAVAGVLTGLVPYAQLNVPDPIAKGVDAIGVTWFSVLIKIGALTGLT
                     TVILVLLYGQSRIFFTMSQDGLLPHLFARVHPRLQTPHLSQMMIGAIVAIVAAFTPIN
                     VLGEMVSIGTLFAFILVCGAVIYLRRSDAGASRPFRAPGVPVLPILGILFCLLLMAGL
                     PLVTWLRLFVWLVIGLIIYGLYGRNHSRLRHPERAGD"
     gene            complement(3033..3254)
                     /locus_tag="VHV99_09305"
     CDS             complement(3033..3254)
                     /locus_tag="VHV99_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635671.1"
                     /translation="MLLNNLALIGRFMYTRADYLALVSLATSGQIPLDAVELKFYPFA
                     QLEAAIDAAGRMSGLQCTVAEGKSREAWS"
     gene            complement(3273..3464)
                     /locus_tag="VHV99_09310"
     CDS             complement(3273..3464)
                     /locus_tag="VHV99_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635672.1"
                     /translation="MIPVVLSGDATQDAAAIRTAGGGGADLAFDMIGQAAHANATFAA
                     LRSLRRGGRRVLMGSMQVD"
     gene            complement(3759..4108)
                     /locus_tag="VHV99_09315"
                     /pseudo
     CDS             complement(3759..4108)
                     /locus_tag="VHV99_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067725.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            4122..4301
                     /locus_tag="VHV99_09320"
                     /pseudo
     CDS             4122..4301
                     /locus_tag="VHV99_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611267.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4374..5027)
                     /locus_tag="VHV99_09325"
     CDS             complement(4374..5027)
                     /locus_tag="VHV99_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635673.1"
                     /translation="MSMFKDAFTQMNSDYGDATQQLPGTAYAPWLAQSDQAMLTGLAD
                     FTASITDTSGSPNPLLPNQKDTFSYQVSQSTSTKGDLMNGAISQNQQSHLRASYHEAL
                     SGGPVELDTSPASQNYNYIQISDDASSTVNIETERGNLLLASLNQTSSQSTRQSQYVR
                     GMLTSDVTTPTSTSNSKDLLALLKPFLEDNQAAQDSAAWQQTLSGIHGMILLNARAN"
     gene            complement(5118..5732)
                     /locus_tag="VHV99_09330"
     CDS             complement(5118..5732)
                     /locus_tag="VHV99_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635674.1"
                     /translation="MEPTITWAQSNTDAVSQVMAANYMAGTMSGQFNNLGSALLDRFT
                     TTGSDFSQSVTVGLAGATGAVDAVGASSNGPQDHIRLTVQTASGVEVDIRIDSGNGTL
                     GVSVHSSETLSDTERTALAKLADGFQNAINGLSASPPELDLSGLTQFDPSVLSSVDMQ
                     FNVTGDGQNNISAEFSQDGSARSVNLTDSVGTVDLKVDTSDSAL"
     gene            5794..5958
                     /locus_tag="VHV99_09335"
     CDS             5794..5958
                     /locus_tag="VHV99_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635675.1"
                     /translation="MTIVDEDPVTAPCDARTVAARLFAVFDTGVTAEGRTAFNDNKVV
                     MVRCFVIFGG"
     gene            6388..8007
                     /locus_tag="VHV99_09340"
     CDS             6388..8007
                     /locus_tag="VHV99_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX3635676.1"
                     /translation="MHKLSFTQKLWLPLIISLIALLAVSVSAAWQSRETRIEERKHDL
                     TNIAHVGLSIVTEYAALAQSGALPEADARKQALERLRGVRYGEDGYFLVINSKPQMVM
                     HPIKPALEGKDLAGSADADGRHHYVTFASTAQAPEGGFVDYVFPHSGAASTQAVGKIG
                     YVVRYAPWDWIIATGAYVDDIDAAFMASLWLIGAIFVAVSAVLVTLVAVTNRSIARTV
                     GGDPAYAANVADAIASGDLTVAIRTRDGDTSSLLHVMQRMRDALTGTICQIKHASDNV
                     ACGANEIANGNADLSARTESQAASLQETASSMEQMTAMVRQTADNARTASELAASAAK
                     IANRGGDMITQAVAAMKGISNESGRMVEIIATIEGIAFQTNILALNAAVEAARAGEQG
                     RGFAVVASEVRSLAQRSATAAKEIRELISHAVGSVGSGATLVENTGSTIDEARDAIMR
                     VTGVVQEIAAAAAEQSAGIDQVNLAVTQMDSMTQQNAALVEQAAAAAQSLKEQAMSLQ
                     RAAAAFQVDDADASGGAPLAMASAQHRAVFA"
     gene            complement(8163..9344)
                     /locus_tag="VHV99_09345"
     CDS             complement(8163..9344)
                     /locus_tag="VHV99_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011694206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX3635677.1"
                     /translation="MKNAVFISVVTGMFATAAHAQNSVTLYGVIDAGISYISNQKVAG
                     STAGHSNFAMTNYSTNPDLFGLRGSEDLGGGLKAIFTLENGFTLTNGVLGQHGVLFGH
                     QAFAGLSSDEVGTLTLGRQYDAMVDYLAPLTTASVNNGGEFFGHPYDNDNLENTMRIN
                     NSVKYASVDYGGWRFGALYGFSNAAGSFANNRAYSFGTSYQNGPVHFAASYLQLNNGG
                     SLTNLTGATDSYVSDGKLTGSDAAFSAGQQRTFGAGLNYTFGPAIFGFVLTETKLENA
                     TEINNTNSSSIALGGSRLRFDNYEINARYSPTSTLKLIGSYTFTDGSSSLAGASDPKW
                     NQVNLQLDYALSKRTDVYVEGQFQHVSGGGNVFTADIYGLAPSANNKQLAVTAGLRTR
                     F"
BASE COUNT         1803 a         2837 c         2910 g         1919 t
ORIGIN      
        1 aaaggtgcct gcatgagcgt cccgcaacaa gccttcctcc gcgatgcgat gcgtcgcatg
       61 aacatgaccc gcgacacgtt cgcgagccgt atcggcgtat cgcgccgggc gctcgacacc
      121 tggctgctgc cggacgattc gcaggaatcg cgcgccatgc ccgaaattgt cgagcgtttc
      181 gtgtcggaaa tcgccgtcca cggcgagccg ggtgaaaaat atacgcaaag cgtagactca
      241 caatcgctgg cgagccagat gctgttcgag ggcaagccgc aactgctgtc ggtcgatcag
      301 ttctcgcgcg actccgtcga ggcgctgttt cgcgtcgccg acatcatgca gccgatcgca
      361 cggcgccgga aaatctcccg cgtgctcgag ggcgcggtgc tcggcaatct gtttttcgaa
      421 gccagcaccc gcacccgcgt gagctttggc gcggcgttct gccggctcgg cggctcggtg
      481 tgcgacacca ccggcttcac gttttcgtcg atggcaaagg gcgagtcgat ctacgacact
      541 agccgcgtga tgagcggcta cgtggatgca ctggtgatcc gtcatccgga gcaaggctcg
      601 gtggcggaat tcgcgcgcgc caccaacgtg ccggtgatca acggcggcga cggtccgggc
      661 gagcatccga gccaggcgct gctcgatctg tacacgatcc agcgcgagtt ctcgcggctg
      721 ggcaagatcg tcgacggtgc gcatatcgcg ctggtcggcg acctgaaata tggccgcacg
      781 gtgcattcgc tagtcaaact gctggcgctg tatcgcggca tcaagttcac cctgatctcg
      841 ccgcccatgc tcgaaatgcc gagctacatc atcgagcaga tctcgcgcaa cgatcacgtg
      901 atcgagcaga cccacgatct gcccgccggg ctgcgcggcg ccgacgtggt ctacgccaca
      961 cgcatccaga aagagcgctt cgccgacgaa tcgttcgaag gctatacgcc ggacttccag
     1021 atcaatcagg cgctgatgga cagcgtatgc ggcgccgaca cgctgatcat gcacccgctg
     1081 ccacgcgaca gccggccggg cgcgaacgat ctgagcgtcg atctgaacca tgattcgcgt
     1141 ctcgcgattt tccggcaaac cgataacggc atcccggtgc ggatggcgat cttcgcagtg
     1201 ctgctgggtg tcgaaaaact ggtccagcat tcgatgcgcg acgccgcgtg gcgcccgccc
     1261 gcctatctcg gtccggacga tgcggtgttt cacggcatcg attgagcagg actctgatga
     1321 ttgaaacgcg gccttgcggt tcacacgcaa ggccgcttct gcattcacac atccatctcc
     1381 agtatccgac cgattaaatc cggactagac tgaattcgtc agctccctct tcaaccgcag
     1441 catcaacggt gccggccgcc ttcaaagaaa tcgcttattg cagtgcgtca cggatcgatt
     1501 cgatcccgga ttcacccaca tacgaacagg acgactcatc atggccgaca ccagttatat
     1561 ggcgcgcaaa tccatcgcgg atattgtcgc aagcgcggag ggcggggaac gccagttgtc
     1621 gaagtcgctg ggcgcgctca gtattacggc gatgggtatc ggcgcgatcg tcggcgccgg
     1681 catctttgtc ctgaccggca cagccgcggc ccactatgcg gggccgagca tcattctttc
     1741 gtttgtcctc ggcgggatag cctgcgcgtt tgtcggactc tgctactccg agctggctgc
     1801 catgctgccg gtctgcggca gcagttatac ctacacctat gccacactcg gcgaattatt
     1861 cgcatgggtc atcggttggg atctgatcct cgaatatgcg atgggcgcgg cgaccgttgc
     1921 ggtcggctgg tcaggctaca tcgtgagcct gttgcataac gtcggcatta acattccgcc
     1981 cgccatggcg gccgcgcgcg gcaccacggt gacgctcgcc gatggcagca cggtggccgg
     2041 cgtcgtcaat ttgccggcgg tcttcatcat cgccgtgctg accatcatgc tcgtgctcgg
     2101 caccaaggag tcggcgcggc tcaataacat catggtggcg atcaaattga ccgtggtgct
     2161 ggcgttcatc gtcatcggcg cgttctttgt gcatcctgcc aactggcatc cgttcattcc
     2221 gcaaaatacc ggcgaattcg gcaatttcgg cgtaagcggc attctccgtg gatcggccgt
     2281 cgtattcttc gctttcatcg gcttcgatgc ggtgtccacc gcggcccagg aagcacgcaa
     2341 gccgcaaatc gacatgccga tcggcattct cggttcgctg gcgatctgca cggtgctcta
     2401 cattgcggtc gccggggtgc taactggact cgttccctat gcccagctca acgtcccgga
     2461 tccgatcgcg aaaggtgtcg atgcgatcgg cgtcacgtgg ttttccgtgc tcatcaagat
     2521 cggcgcgctg actggcttga cgaccgtgat actggtgctg ctgtatggcc agagccgcat
     2581 cttcttcact atgtcacagg acggcctcct gccgcacctg ttcgcgcgcg tccacccgcg
     2641 tttgcaaaca cctcatctga gccagatgat gatcggcgcc atcgtcgcga tcgtggcggc
     2701 attcacgccg atcaacgtac tgggcgagat ggtcagcatc ggcaccctgt tcgcgttcat
     2761 tctcgtctgc ggcgccgtga tctatctacg ccgcagcgac gcgggagcct ccaggccgtt
     2821 ccgcgcgccg ggcgtgccgg tgttaccgat cctcgggatt ctgttttgcc tgctgctgat
     2881 ggcagggctg ccgcttgtta cctggttgcg gctgttcgtc tggctcgtga tcgggctcat
     2941 catctatggg ctctacggtc gcaatcattc gcgtcttcgc catccggaac gggctggcga
     3001 ctgagcgcgc agcgcagcgc agcgcagcgc gctcaactcc acgcctcgcg agatttgcct
     3061 tccgcgaccg tgcattgcag gcccgacatg cggcctgcag cgtcgattgc cgcttcgagc
     3121 tgggcgaatg gatagaactt caactcgacc gcatcgagcg gaatctgccc ggacgtcgcg
     3181 agcgaaacca gcgcgaggta gtcggcgcgc gtgtacatga agcggccgat caacgccagg
     3241 ttgttcagca acatctcgcc gtagggaatc ggctaatcca cttgcatgct gcccatcagc
     3301 acgcggcgcc cgccgcggcg caggctgcgt aacgcggcaa aggttgcgtt cgcgtgggcg
     3361 gcctgcccga tcatgtcgaa cgcgaggtcc gcgccaccac cgcctgcggt gcgaatcgcc
     3421 gccgcgtctt gcgtggcatc gccgctcagc acgacgggga tcacacgccc gcggccctgc
     3481 tcaacatgaa atgagatccg cttgacgtag ctccaaaggg agctcagttt aacgtctcaa
     3541 atcgcacgtt aatacgagtt atcttggcaa accatctctc acatctaatc gtgcgagaac
     3601 tgaggggttg cagccgcccg ttcagggctc gaaggactga gatcgacccg gagcggaccg
     3661 tgatcctgct ccaaagcggt cactcaaatc gctggcggag tatcggtgcc ccttgatcgg
     3721 ggtgacggga agtgctgaca gattgcagat gacgcatatc acgcatcgct cagaagcacc
     3781 actgcttgtg cctcttcgac ggctgcgagc tgcggatttt cgggttccag ttcagggcgg
     3841 gtcttaagcc aaagtccgaa ttcgcgcatg acctcgacga cgggttttag ccgccggcca
     3901 tcctcggtga gctgatactc cactttgcgg gcacttcagc gaatacggtc cgttggatca
     3961 agcctacctg ttccagtgct cgcaaatcta gcgtcaacat gcgctgcgat attcccggga
     4021 catccctgcg cagatcatta aaccgcttcg gcccgtcagg caaatacgac accagcagca
     4081 gacgccagcg accacccagc aagcgcattg cttcttcaac cgtgttgaaa accggtttgc
     4141 gatggaacca tctgccgaca gtgttgggct ttggctccgg cgcgacctgc tggcgacgac
     4201 tgaacgactg gcgcaaagca ggagtatggg atcgactgca cgaactgctg ctcgacaagt
     4261 tgcgcggggc cggacagatc gatctttcat acgctgccgt aaagaggatc gctctcgtta
     4321 cttgcaggtt cgggagcggc attgtttacg tcgccccttt gccgccggct atttcagttc
     4381 gctctcgcgt ttagcaaaat catcccgtgt atgcccgaaa gcgtctgttg ccacgcggcg
     4441 ctgtcttgtg cggcctggtt atcctcaagg aaaggtttca gcaacgccag cagatctttc
     4501 gaattggagg tgctggttgg cgtggtgaca tctgacgtca acatgcctct cacgtattgc
     4561 gattggcgtg tggattgact ggacgtctga ttcagcgatg ccagaagcag gttgccgcgt
     4621 tccgtctcaa tgttgaccgt gctgcttgcg tcgtcactga tctgtatgta gttgtagttc
     4681 tgtgacgccg gcgatgtgtc gagttcgacc ggaccgccgg atagcgcctc gtgataactc
     4741 gcgcgcagat gcgattgctg gttttgcgaa atcgcgccgt tcatcagatc gcctttggtg
     4801 ctggtgcttt gagagacctg ataggagaaa gtatcctttt gattgggcag caatggattt
     4861 ggcgaccccg aggtatcggt aatcgaggcg gtgaaatcgg cgagacccgt caacatggcc
     4921 tggtccgact gcgcgagcca cggcgcatag gctgtaccgg gaagctgttg cgtcgcgtcc
     4981 ccatagtcgc tgttcatctg cgtgaacgcg tctttgaaca tcgacatcaa cgctgcgttc
     5041 ccgtgacctt tcgcgttggc gttgtcgaac tgcgcgagat agctcgcgat ggcctgatcg
     5101 cgttgcgcgc ccgatcccta gagtgcggaa tcgcttgtat cgactttgag gtccaccgta
     5161 ccgaccgaat ctgtgaggtt tacgcttctc gctgaaccgt cctgggaaaa ttcggctgag
     5221 atgttgtttt gcccgtcgcc cgtcacattg aactgcatgt ccaccgacga caaaaccgac
     5281 gggtcgaact gcgtcagacc gctcagatcg agttccggcg gggaagcgct aaggccgttg
     5341 atcgcgttct gaaaaccgtc ggccagcttg gccagcgcgg tgcgctcggt gtcactcagc
     5401 gtctcgctgc tatgcacgct gacccccagc gtgccgttcc cgctatctat cctgatgtcg
     5461 acctcgacgc cgctcgcggt ctgaacggtc agcctgatgt ggtcttgcgg accgtttgac
     5521 gaagcaccga ctgcatcgac cgcaccggtt gcgcccgcca aaccgaccgt gaccgactgc
     5581 gagaaatcgc taccggtagt cgtgaagcga tcgagcagcg cgcttcccag gttgttgaac
     5641 tgccccgaca tcgtgccggc catgtaattg gccgccatca cctgagagac ggcatcggtg
     5701 ttgctttgag cccaggtgat ggtcggttcc acacctgcca ggattccttg ctgcgtgtag
     5761 aggacgggcg ctgcgacgat cgtcgaggga atcgtgacaa tcgtcgacga ggacccggtg
     5821 acggcgcctt gcgatgcgcg aactgtcgca gcaaggttgt ttgccgtgtt tgataccggc
     5881 gtcaccgccg aaggaaggac tgcgttcaat gacaataaag tggtcatggt gcggtgcttt
     5941 gttattttcg gcggatgatg gccgtcctca acgcgacgac cggcttcagt agggttatcg
     6001 acaggaagtt gaaaatcctt aatgcagagg gattgtcagg caattgaagc atgagccgga
     6061 tgtgttttcg gtcgaccatg cctcctatca gaaagctcag acagaaaatt gtcagcaatc
     6121 ttaccgaggt ggtcgaccgt tcgaatctgg ccggctagcg cctgatgacg ctcggtggga
     6181 gccgacccac aagggccctt cgatacgcct tggcacggtg gtaaccaagg gtttcacagg
     6241 atgaaacttt cgctggtatc cgccgacaac ccggtcagga gccccttgga cgccggacag
     6301 tcagcggcgc cacccggcgc gcccatacac atgccacttt gcacggcagc cctacgcgat
     6361 ttccattcgt tttcaatagg actcaggatg cacaagctca gttttacgca gaagctgtgg
     6421 ctgccgttga tcatcagcct catcgcgttg ctcgccgtct ccgtatcggc ggcgtggcag
     6481 tcgcgcgaga cgcgcattga ggaacgtaag cacgacctta cgaacatcgc gcacgtcgga
     6541 ctcagcatcg tgacggagta tgcggcgctg gcgcaaagcg gtgcgctgcc ggaggcggat
     6601 gcccgcaagc aggcactcga acgcctgcga ggcgtccgct acggcgaaga cggctacttc
     6661 ctcgtgatca actcgaagcc gcagatggtg atgcatccga tcaagccggc tctcgaaggc
     6721 aaggatctcg caggcagcgc cgatgccgac ggccggcacc attacgtgac gttcgcgtcg
     6781 actgcgcagg cgccagaagg cggcttcgtc gactacgttt tcccgcattc gggcgcagcg
     6841 tcgacacagg cggtcggcaa gatcggctac gtggtgcgct atgcgccgtg ggactggatc
     6901 atcgcgaccg gcgcctatgt cgacgacatc gacgctgcct tcatggcgtc gctgtggctg
     6961 atcggcgcga tcttcgtggc cgtgtcagcg gtgctcgtga cgctcgtcgc ggtgacgaac
     7021 cgcagcattg cgcgcacggt cggtggcgat ccggcctacg cggcgaacgt ggcagacgcg
     7081 atcgcatcgg gcgacctgac ggtagcgatc cgcacgcgcg acggcgacac gtcgagcctg
     7141 ctgcacgtga tgcagcggat gcgcgacgcg ctgacgggca ccatctgcca gatcaagcat
     7201 gcgtccgaca acgtagcgtg cggcgcgaac gagatcgcca acggcaatgc cgacctgtcg
     7261 gcgcgcaccg agagccaggc ggcgtcgttg caggaaacgg cgtcgagtat ggaacagatg
     7321 acggcgatgg tccgccagac tgcggacaac gcgcgcaccg ccagcgagct tgcggccagc
     7381 gccgcgaaaa tcgcaaaccg cggcggcgac atgatcacgc aggctgttgc ggcgatgaag
     7441 ggtatctcga acgaatcggg ccgcatggtc gagatcatcg cgacgatcga gggcatcgcg
     7501 ttccagacca acatccttgc gctgaatgcg gcggtcgaag cggcacgcgc gggcgagcag
     7561 ggccgcggct tcgcggtcgt cgcgagcgag gtgcgcagtc tcgcgcagcg cagcgcgacc
     7621 gcggcgaagg aaattcgcga gctgatcagt catgcggtgg gcagcgtcgg cagcggtgca
     7681 acgctggtcg aaaacaccgg ttcgacgatc gacgaagcgc gcgacgcaat catgcgtgtg
     7741 accggtgtcg tgcaggagat cgcggcggcc gcggcggaac agagcgccgg catcgaccag
     7801 gtgaatcttg cggtcacgca gatggacagc atgacacagc agaacgcggc gctcgtcgag
     7861 caggcggcgg ccgcggcgca gtcgctgaag gagcaggcga tgagcctgca acgcgctgct
     7921 gcggcgtttc aggtggacga cgcagacgct agcggcggtg caccgctcgc gatggcttcg
     7981 gcgcagcacc gcgcagtgtt cgcgtaagga cccgcgccgc aatctcgcgg ggggggcgtg
     8041 cccacgatcc gacacgcctc aaagtcgaat ccgaactttg aatagagcaa cggcgaaagc
     8101 tgcgatcagg gcgagcagat cacgaattgc acggtgccat cttgatccgg gtttgacgga
     8161 gttcagaatc gggtacgcaa gcccgcggtg accgcgagtt gcttgttgtt cgccgaaggc
     8221 gccaaaccgt agatgtcagc cgtaaacacg ttgccaccgc cgctcacgtg ctggaactgc
     8281 ccttcgacgt acacgtcagt ccgcttggac agcgcgtagt ctaactgcag gttgacctga
     8341 ttccacttcg ggtccgaagc gcccgcgagg ctggaactgc cgtccgtgaa cgtgtatgac
     8401 ccgatgagtt tgagtgtcga agtcgggctg tagcgtgcgt tgatttcgta gttgtcgaaa
     8461 cgcaggcgtg agccaccgag agcgatcgaa ctggagttgg tgttgttgat ttccgttgcg
     8521 ttttcgagct tcgtttcggt gagtacgaag ccgaaaatcg ctggaccgaa cgtgtagttc
     8581 aaaccggcac cgaacgtgcg ttgctgaccg gcgctgaacg cggcgtcgct gccggtcaac
     8641 ttgccgtccg aaacgtagga gtctgtcgcg ccggtcagat tggtgagact accgccgttg
     8701 ttgagctgca ggtacgacgc cgcgaaatgc actggaccgt tctgatacga cgttccgaag
     8761 ctatatgccc ggttgttggc aaagctgccg gccgcgttgg agaagccgta gagggcgccg
     8821 aaccgccagc cgccgtagtc cacgctcgca tacttgaccg agttgttgat ccgcatcgta
     8881 ttctcgagat tgtcgttgtc gtacgggtgc ccaaagaact ccccgccgtt gttgacggag
     8941 gcggtcgtca acggtgcaag gtagtcgacc atcgcatcgt attggcggcc gagagtcaga
     9001 gtgccgacct cgtcgctgga gagacccgcg aatgcctggt gaccgaacag cacaccatgc
     9061 tggcccagta ccccgttggt gagggtgaag ccgttttcca acgtgaagat tgccttgagg
     9121 ccgccgccca gatcttcgga accgcgcaga ccaaaaagat ccgggttggt gctgtagttg
     9181 gtcatcgcaa aattgctgtg ccctgcagtc gaacccgcta ccttctggtt cgatatatac
     9241 gatatccccg catcaattac accatacaac gttacgctgt tttgcgcatg cgccgcagtc
     9301 gcgaacatgc ctgttacgac cgatataaaa accgcgttct tcattctatt gcctattgaa
     9361 tcaccaaagt atgaatggaa accaaggtac gatttctgac ggcggattca gtactttggt
     9421 ttccattcat actttggtga ttcaataggc aatagaatga agaacgcgg
//
LOCUS       DATASH010000336        15984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5188995,
            whole genome shotgun sequence.
ACCESSION   DATASH010000336 DATASH010000000
VERSION     DATASH010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 15984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15984
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5188995"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..131
                     /locus_tag="VHV99_09350"
     CDS             <1..131
                     /locus_tag="VHV99_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEX3635678.1"
                     /translation="RRGTDPYNSMSHLGFRLVMDEDTWKQPLARQASARAADADGS"
     gene            221..1363
                     /locus_tag="VHV99_09355"
     CDS             221..1363
                     /locus_tag="VHV99_09355"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006164690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX3635679.1"
                     /translation="MRSSCEPRWSERAAGSIRAAGEATFGSACRSTFRSTFRSALGAT
                     LVALSVTLGAAGCTTPGVTNAPSPPAAVSSTAALPSWRDGTAKQTIEKFVADVTRAGS
                     PNFVPPAERIAVFDNDGTLWSEQTLYFQFAFMLDQVKAAAPKHPEWKNNPAFKALVAK
                     DYAALASQQKQVLQLVAVANSGMTVDDYDKTIHDWLASARHPKFNRPYTELVYQPQLE
                     LLAYLRANGFKTFIVSGGTIEFMRPWAEKAYGIPPEQVIGSSQLVQYQMHDGQPALVR
                     LPKLDFVDDGPGKPVGIYRNIGRKPILAFGNSDGDLQMLQYTASGSGPHLALLVHHDD
                     AVREFAYDRTSKIGKLDKTWDEAIVDGWTVVSMKDDWQTIYPAPKQ"
     gene            1475..3196
                     /locus_tag="VHV99_09360"
     CDS             1475..3196
                     /locus_tag="VHV99_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEX3635680.1"
                     /translation="MRMFLRKGRYAAGATVVALAAFAALILPSINVPAQTQAPAKPAP
                     KPAAAPATAAATSAPQQQPAARNSSKPNILVIFGDDVGQANISAYTRGVVGYKTPNID
                     RVANEGMIFTDYYAENSCTAGRSSFITGQSPLRTGLSKVGAPGAPVGLQKTDMTIAQA
                     LKPLGYATGQFGKNHLGDKNEYLPTNHGFDEFYGNLYHLNAEEEPERPYWPKDKNDPY
                     AKNFSPRGVIHSTSDGKVQDTGPLTAKRMETIDDETGAHAEEFIRKQVKNGTPFFVWM
                     NYTRMHVFTHVRPEFRGKSGMPGNEYADGMWEHDQDVGKLLKLLDDLKIANNTIVIYT
                     TDNGPNQFSWPDAATTPFRSEKDSNWEGAFRVPAMIRWPGHIKAGETSNEMISGLDWF
                     PTLLAAAGDSGVTDRLLKGWSPKAGGASFKNHLDGYNQLPFLTGQTTRDPRTEFYYFD
                     DDGDLVAMRWDQDGNAWKAVFCEQRAKGNLNIWQDPFVCLRLPKIFNLRMDPYERADV
                     TSDQYNDWLAKNAYLTGIATMKASAFLQTFVSYPPSQRPASFSVDGVRRQVDRKIDES
                     FKKRGLE"
     gene            3406..6066
                     /locus_tag="VHV99_09365"
     CDS             3406..6066
                     /locus_tag="VHV99_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEX3635681.1"
                     /translation="MHEPQKEAAGPGLPIAWPLLLLFASGGASLIYQVLWIKQLALVV
                     GVEVQAVSTGVSAFFAGLAIGGWVFGRLADRLARPLRLYALLEAGVLVLALASTWMLP
                     HAAAPFASLQDRIGPLAWALPFGLVGLPAVLMGGTLPVLMRALCPAAIHLGRAGARLY
                     AANTAGAIAGTLAASFVLIPWLGVFGSACAAAVLNAGAALVAGLLGRMQQALLPVDES
                     TSSTVASARSTADIAHDTRSPVAAVRTARDASSPAAAARTARDASSQSPRADIARNTS
                     VSKGRLALVLYAIAGGVALGYEVVWSQVIVQFISTRSFAFAVVLATYLLGLALGSALA
                     SRYADRARDPWGAFATLIVAAGLVALLEIAVLGDWLPHWQSLAREAVLGATDSLLSAM
                     CAGFAVAAVCVVFVPTLLLGAAFPFALRLNVDNRHTGRDVGAVVALNTAGGIAGTLLT
                     GFVLVPMLGLVHTLAALAVVAGAMSLIAVLRGSDLRPFARWSVPVLAVLTLITVFVTP
                     SDRLATLLAESRGGHLTFYEESAGGTVAVVEQNAGQRQFRRLYIQGVSNSGDTMASLR
                     YMRLQALLPLLIHRETPRAALVIGLGTGITGGALLTWPGLEHRTVAELLPAVVRAAPQ
                     FKGNYSMSTDGRMDIRMRDGRRELLRNAERYDLITLEPPPPSAAGVVNLYSSDFYRLA
                     ASRLQQGGIVAQWLPLPTQNEQDTRALIQSFIQVFPHAALWTTELHEMMLVGSMQPLE
                     LDVPRIEARFAQPRVAAALREVGIASPAALLATWITDRAGLAYYAADAQAVTDDQPRI
                     EYAPWVRHDAFPTTLTHLLALHTEPPLQGADDAFRAAMENSRGALHSFYAAGLDAYKG
                     DRDAWAQDIGDALRTDPGNAYYRWVVGDGN"
     gene            6128..7246
                     /locus_tag="VHV99_09370"
     CDS             6128..7246
                     /locus_tag="VHV99_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008354395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEX3635682.1"
                     /translation="MNDNPPDAATEGKKFLREILRPVAAINRWLDQLDPGTHRRIKGL
                     RLVTAYGIAAALGTLQDIAHSVPSNVSLGYLAGSFALWASVSEGKTTRAESSRDLLLL
                     SLAAGLGAALFAVCFGPLQLAGRAGPELMLVTGAFLVGYLKRFGVLGAGIGSQIYLGE
                     LFAYSAHLTTADLWAIAIAALIAALASIVPRVLSGPAERPASLPALAAGMAPGAGRWS
                     PEFFMGVQAALGALVIVVLNGMIGLEESAWAITACTYVVAGSATGTIQRVRRRILGTM
                     VGVPLGLACLPVAEHAPLLIWIAAALAMIVYAMALPERYDIACGAFAFTLIVTLAASG
                     VHSIPLLAARAWETLLGGALGLLAAMFVFPLRTSGPGK"
     gene            7465..7743
                     /locus_tag="VHV99_09375"
     CDS             7465..7743
                     /locus_tag="VHV99_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635683.1"
                     /translation="MHRTRHLPAIAALSLSLQGCALRGAPSYEISGAYFPLWLVSALV
                     GFVGALIAHRIFVSTGWAQIVPFQLSVCVAIGLTVAVLFWLSGTGQLL"
     gene            7740..8789
                     /gene="mdtN"
                     /locus_tag="VHV99_09380"
     CDS             7740..8789
                     /gene="mdtN"
                     /locus_tag="VHV99_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006751291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtN"
                     /protein_id="HEX3635684.1"
                     /translation="MKIAGKRQASVKGRVIAGVIVLLGIAAAMYGYDRTTRFPSTDDA
                     TIDADVVHVASPVGGRIVRLAVQENQRVAKGDVLFEIDPVPFRLVVAQTQADLELARA
                     ALETRRRTIVGERANATIAGDQTGKAAHNYELAARTVQRLTPLVAKGYVPVQQLDQAQ
                     VAERDAALSLKQAQKQKQASAQTIGDEADAVAVVHAREAALALAQHSLDDSVVRAPQN
                     GFVTGLSVLAGETVAPHQSIFTLVHADEWFAVANFREGALARIQPGDCATVYSMIDRS
                     QAIHGKVVGIGAGVADSDRINLPRTLPIVQQSVNWVRVAQRFPVRVRLDEPAARLVRI
                     GASAIVEVRHGAACR"
     gene            8770..10995
                     /locus_tag="VHV99_09385"
     CDS             8770..10995
                     /locus_tag="VHV99_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014898915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEX3635685.1"
                     /translation="MAQPAGDLARPGPADVLKLLAPYPGRAAMAARIALICALTAFVT
                     SAYGTPEAAISAYVVFFLNRPDRVLSVVLGSALLVLVTLVVGLVMVVAMFALDEPLWR
                     VACITVLSFGFLFVTSASKLRPVGAIIAMIVGFALDELGMAPFGEAATRALFYAWLMV
                     AIPIGISIGVNLLVAPSPRKLAGRELARRLRLAARSLAEPESTGDALSACLREGEADL
                     GTWLKLSVADGSSRRADVAPLRQAVSSTLAILVAADLSVHEPSARLPASFVGPIAGTL
                     ENMARMLEAGGYPVDIELTLPAVTGLTPLAHIVVENLHDAIVRFAVVESEVAVGAEGV
                     IQTEGRAEGRPEDRAEGRPEAGAEGRAEADAEARPDARAEGRAEADAEARPEARAEGR
                     AEADAEARPEARPEAITAPPADGKRGGFFLPDAFTNPDHVRYALKTTVAAMVCYLLYS
                     QIDWPGIHTCFITVYMVSLGTTAETVEKMTLRIAGCMVGALLGVAAILFVVPVLSSVV
                     GLMALVLMGAWLSAWVAFGSPRIAYAGFQIALVFFMCVLQGAAPGYDLTIARDRTIGI
                     LIGNVVVYLVFTRVWPVSVAERVDTGLAALRQQWQRLAALPDAVTRRAQAASAMAQCG
                     ALEQDLALMHYEPSWVRPESQWIAERRSALARLDALEGPMFLLAERRPGDAAIDGWLG
                     RVAGGLPLALAPAETIEPNAAADDSARTALVNLGDARLTQLEHAASNDAAKELTSHAP
                     A"
     gene            10982..12649
                     /locus_tag="VHV99_09390"
     CDS             10982..12649
                     /locus_tag="VHV99_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017328977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX3635686.1"
                     /translation="MRRLKPPFAVATLVAAGLAGCATSSIDMAPDSPDRPWQPQTDAA
                     GAIVPGPAGPPGPAGSAGSAGSAGSAGSAGSAGSAGSAGSPNPANTTAPANPTPSARS
                     YQLPANPALAVVPPPPPLEQEHAYGLPELIDLAESANPLTRIAWNDARNVALAAGIAK
                     SAYLPQLSVAAMGQYGGFQGSSTSVLGDSSVSATGHGTTSVVALQWLLFDFGGRAARV
                     EAASQASVMANIGFTAVHQQVIFDTSVAFYAYQAARARIATTRLGLDNAEAILAAAQS
                     RYKHGIGTVIEVAQANQNRAQARLALVQAQGVESNSYLALIAAMGISPLSKPRIADVP
                     AHTLPASTSDSIERIVSTAIARRPDVLSAYAAERVNLAKVKAAESDFMPKVFVSATGA
                     YNTGSSSISAVPAVGQQLPTVNLSGGRYGGSVIVGVTIPLYDGGLRSAVLAQARNDAD
                     SASTRLTRSREEAVRQIVAAQNTLQTSLAAQAAARELLAAAQTTYDAAFDAYRHGVGS
                     VTDALLAQNQLLAAKNADADSYSSALSAAAALALATGSVDSIPMRDN"
     gene            complement(12742..13356)
                     /locus_tag="VHV99_09395"
     CDS             complement(12742..13356)
                     /locus_tag="VHV99_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011494308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX3635687.1"
                     /translation="MNTRDTPLRLTDRKRAAVIAAAIEEFLAAGFDATSMDRIAARAS
                     VSKRTVYNHFPSKEVLFAAILRQLWESSDTGEAPVYSAVHPLRAQLLQLLLKKLGLLN
                     DEAFLSLARVAIAAGIHSPERARDMVTRMGEREEDLTIWIRAAAADGRLKTSDPMFAS
                     QQLQGLVKAFAFWPQVTMGHPPLSVAEQRQVAESAADMFLARYA"
     gene            13440..14564
                     /locus_tag="VHV99_09400"
     CDS             13440..14564
                     /locus_tag="VHV99_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX3635688.1"
                     /translation="MTSFTGSIRRALGLAAARRGRELFDTSAQHDGERFRNVKPRPVE
                     GIGKTLSIVWNVLFNKPDDTTPAGALPVDALSREQLDAAPERSLYRLGHSTMLLKLRG
                     QFWLTDPVFAERASPFRRFGPKRFHAPPIALEDLPPLRAVILSHDHYDHLDRETVLAL
                     AARTAVFLTPLGVGDRLIEWGIEAAKVRQFDWWQSVEIDGLAFTATPAQHFSGRSLFD
                     GNSTLWASWVIVDDALRVFFSGDTGYFDGFKSIGERLGPFDVTLLETGAYDAQWPYVH
                     MQPEETVQAHVDLRGRWLVPVHNGTFDLAMHRWQEPYERVIGLTAVRGIPLATPRMGE
                     RLDLGAPHRGEKWWRAVKAPAQEPVTSRRWFACRNSRQAN"
     gene            complement(14622..15983)
                     /locus_tag="VHV99_09405"
     CDS             complement(14622..15983)
                     /locus_tag="VHV99_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3635689.1"
                     /translation="MAARIERLPFSGFHRRLLLMGGLGYTFDAMDAAVLAFLLPVLRT
                     QWGLTSVQTGVLGSGTFMGYFVGAMLAGMLGDVIGRRKVMMYALVIYCIASLASAVAN
                     DWGFFLATRIVAGLGTGAESAIVAPFLSEFVARRYRGSFTGSLAGFFSFGFVAAALLG
                     YLVIPLAPNAWRAVMVITALPIVMLLWWRRALPESPRWLEARDRHAEADAIVSRMEAE
                     VIKGGVQLEPLPAVRDEHVPLAAGRASILANVKALWAAKLARITAMTWLMWLSITFSY
                     YAFFTWIPGLLVQNGMTITRSFSYSLVIYIAQIPGYFSGAWLNEKIGRQATIASYMIL
                     GGVSALGLALTGTDAGIMVSGILLSFFMNGTYAGVYAYTPEVFPTDVRATGTGLASSI
                     GRLGAIAAPILVGYVYPLLGFAGVFGATTLVLLIGALAVVLMGVPTRGRSLEDIAAGQ
                     VER"
BASE COUNT         2780 a         5217 c         5171 g         2816 t
ORIGIN      
        1 cgcggcgcgg caccgacccc tacaacagca tgtcgcatct cggctttcgt ctcgtgatgg
       61 acgaagacac ctggaagcaa ccgcttgcac gtcaggcatc ggcgcgcgcc gccgacgctg
      121 acggaagcta gggcgcgctg ccctttcgag ctggtcccgt ttctcccgcg cgcttgtcgc
      181 tcgcgggtca tggaaattgt gcaatcggaa tggaggttgg atgcgttcga gctgtgagcc
      241 aagatggtcg gagcgtgccg cggggtcgat ccgcgcggcg ggtgaggcaa cattcgggtc
      301 tgcgtgccga tcgacattcc ggtcgacatt ccggtcggcg cttggtgcta ccctggtcgc
      361 gctcagtgtc acgctaggcg ccgccggttg cacgacgcca ggcgttacga acgcaccatc
      421 accacccgct gccgtcagtt cgaccgccgc gctgccgtcc tggcgtgacg gaacggccaa
      481 acaaacgatc gagaagttcg tcgccgatgt cacccgcgca ggctcgccga acttcgtccc
      541 gccggccgag cgcatcgccg tgttcgacaa cgacggcacg ctgtggagcg agcagacgct
      601 gtacttccag ttcgccttca tgctcgacca ggtgaaagcg gcggcgccga agcatcccga
      661 gtggaagaac aatccggctt tcaaggcact cgttgccaaa gactacgccg cgttggcgag
      721 ccagcagaaa caggtactgc aactggtcgc cgtcgccaat agcggcatga ccgtggacga
      781 ctacgacaag accatccacg actggctggc aagcgcccgg catccgaaat tcaatcgccc
      841 ctacaccgag ctcgtctatc agccacaact ggagttgctc gcctacctgc gcgccaacgg
      901 cttcaagacc ttcatcgtgt cgggcggcac catcgaattc atgcgcccgt gggcggagaa
      961 agcgtatggc attccgcccg agcaggtgat cggctcgagc cagctcgtgc aataccagat
     1021 gcatgacggt cagccagcgc tggtgcgcct gccgaaactc gatttcgtcg acgacggtcc
     1081 cggcaaacct gtcggcatct atcggaacat cggccgcaaa cccattctcg cgttcggcaa
     1141 ttcggacggc gatctgcaga tgctgcagta caccgcgtcc ggcagcggcc cgcacctcgc
     1201 gttgctggtg catcacgacg atgcagtacg cgaattcgcg tacgaccgca cctcgaagat
     1261 cggcaagctc gacaagacgt gggacgaagc aatcgtcgac ggctggaccg tggtcagcat
     1321 gaaggacgac tggcaaacca tttatcccgc gccgaagcag taagcggacg cgggagagcg
     1381 cgagccgcgc gcaggttgcg gccgctacga ggcaaccccg tgcgcaggga acgcgtagcg
     1441 ggtgaaccga agtcaactcc attggagcga cacgatgaga atgttcctgc gcaaggggcg
     1501 ctacgcagcc ggggcgacag tggtcgcact ggccgcgttc gccgcattga ttctgccctc
     1561 catcaacgtg cccgcgcaga ctcaggcgcc ggcgaaaccg gcgcccaaac ccgcggccgc
     1621 accggcgact gcagcggcca ccagcgcgcc gcagcaacaa cccgccgccc ggaattcgtc
     1681 caaaccgaac atcctcgtga tcttcggcga cgacgtcgga caggcaaata tcagcgccta
     1741 cacgcgcggc gtggtgggct acaagacgcc gaacatcgac cgcgtggcca acgaagggat
     1801 gatcttcacg gactactacg ccgagaacag ttgtactgct gggcgttcgt cgttcatcac
     1861 cggccagtcg ccgttgcgta cggggctctc caaggtcggc gcgccgggcg caccggtcgg
     1921 tctgcaaaaa accgatatga cgatcgccca ggcgctcaaa ccgctcggct atgcaaccgg
     1981 tcaattcggc aagaaccacc tgggcgacaa gaacgagtac ctgccgacca accatggctt
     2041 cgacgagttt tacggcaacc tgtatcacct gaacgccgag gaagaacccg agcgtccgta
     2101 ctggccgaag gataagaacg acccgtacgc gaaaaacttc tcgccgcgcg gcgtgattca
     2161 tagcacctcg gacggcaagg ttcaggacac gggcccgctc acggccaagc gcatggaaac
     2221 catcgacgac gaaaccggcg cgcacgcgga agaattcatc cgcaagcagg tcaagaacgg
     2281 cacaccgttc ttcgtctgga tgaactacac gcgcatgcac gtgttcacgc acgtgcgtcc
     2341 cgagttcaga ggcaagagcg gcatgcccgg caacgaatac gccgacggca tgtgggaaca
     2401 cgaccaggac gtcggcaaac tgctcaagct gctggacgac ctgaagatcg ccaacaacac
     2461 catcgtcatt tacacgacgg ataacgggcc gaatcagttc tcgtggccgg acgcggcgac
     2521 cacgccgttc cgcagcgaga aggattccaa ctgggaaggc gcgttccggg tgccggcaat
     2581 gatccgctgg ccggggcata tcaaggcggg cgaaacgtcg aacgagatga tctcggggct
     2641 cgactggttc ccgacgctgc ttgccgcggc gggcgacagc ggcgtcacgg accggctcct
     2701 gaagggatgg tcgcccaagg cgggcggcgc gagcttcaag aatcacctcg acggctataa
     2761 ccagttgcca ttcctgaccg gacagacaac cagggatcct cgtaccgagt tctactattt
     2821 cgacgatgac ggcgatctgg tcgccatgcg ctgggatcag gacggcaatg cctggaaggc
     2881 ggtgttctgc gaacagcgtg ccaagggtaa tctgaatatc tggcaggatc cgttcgtctg
     2941 tctgcgtctg ccgaagatat tcaacctgcg catggatccc tatgagcgcg cggacgtgac
     3001 ctcggatcag tacaacgact ggctcgcgaa gaatgcgtat ctgaccggta tcgcgacgat
     3061 gaaagcatcg gcgttcctgc agaccttcgt cagctatccg ccgagccagc gccccgcgag
     3121 cttcagcgtg gacggcgtgc gcaggcaggt cgacaggaag atcgacgagt ccttcaagaa
     3181 gcgcggactc gaatagtacg tcgcgcggtt gcgcctgccg gcctgggacc ggcgggcgca
     3241 acttctggat gccgttgcgt tgcataccgc gtcaagccga ttcatctcta tcgacaccct
     3301 cccctcgcca ttcattccat gacccaaacg ctggccaacc gtgccaaaag ctcacgccgt
     3361 gagcgccgtg cacatgaacg cgcacagcgt cgcgacgatg ctcacttgca tgaaccccag
     3421 aaagaagctg ccggcccagg cctgccgatt gcctggccgt tgctgcttct gttcgcctcg
     3481 ggcggcgcat cgctgattta ccaggtgctg tggatcaagc agctcgcgct ggtggtgggc
     3541 gtcgaggtgc aagccgtcag caccggcgtc agtgcttttt tcgcgggcct ggcaatcggc
     3601 ggctgggtct tcggcagact cgccgaccgg cttgcacggc ccttgcgcct gtatgcgctg
     3661 ctcgaagccg gcgtgctggt gctggcgctc gccagcacgt ggatgctgcc gcatgcagcg
     3721 gcgccgtttg cctcgttgca agatcggatc ggaccgttgg cctgggcatt gcccttcggt
     3781 ttggtcggct tgcctgccgt gctgatgggc ggcacgctgc cggtgctgat gcgcgcgctg
     3841 tgccccgccg ccatccatct gggccgcgcc ggcgcccgtc tctatgcggc caatacggcc
     3901 ggcgcgattg ccggcacatt ggcggcgagt tttgtgctga ttccgtggct cggcgtgttc
     3961 ggcagcgctt gtgccgctgc cgtgctgaac gccggcgcgg cgcttgttgc cggtctgctg
     4021 ggacgcatgc agcaggcctt gctgccggta gatgagtcaa cctcatcgac cgtcgcttcg
     4081 gcgcggtcta cggcggacat cgcgcatgac acgcgttcgc cagtcgctgc ggtgcggacc
     4141 gcgcgcgacg cgtcctcccc agctgctgcg gcgcggacgg cgcgcgacgc ctcctcccaa
     4201 tcgccccgcg ccgacatcgc acgcaacacg tccgtctcaa agggccgcct tgcgctcgtg
     4261 ctctacgcga tcgccggcgg cgtcgcgctc ggctacgagg tggtgtggtc gcaggtgatc
     4321 gtgcaattca tcagcacgcg cagttttgcc ttcgccgtgg tgctggccac ctatctgctg
     4381 ggcctcgcgc tcggcagcgc gctggcgtcg cgctacgccg atcgggcgcg cgatccatgg
     4441 ggtgcattcg cgacgctgat cgtcgcggct ggactggtcg cgctgctgga gatcgccgtg
     4501 ctgggcgact ggctgccgca ctggcaaagc ctcgcgcgag aagccgtgct cggcgccacc
     4561 gacagcctgc tgagcgccat gtgcgcaggc ttcgcggtag cggccgtatg cgtcgtcttc
     4621 gtgcctaccc tgctgttggg cgcggcgttc cccttcgcgt tacgtttgaa cgtggacaac
     4681 cggcacacag ggcgcgatgt cggtgccgtg gtggcgctca ataccgccgg cggcatcgcc
     4741 gggacgctgc tgaccggttt cgtgctcgtg ccgatgctgg gcctcgttca cacactcgct
     4801 gcgcttgccg tcgtcgcggg cgccatgagt ctgatcgccg tgctgcgcgg ctcggacctg
     4861 cggccattcg cgcgctggag cgtgcctgtg cttgccgtgc tgacgctcat caccgtgttc
     4921 gtcacgccat cagaccggct cgccacgctg ctcgccgaat cgcgcggcgg tcatctcacg
     4981 ttctacgaag agagcgcggg cggcacggtg gcggtcgtcg aacaaaacgc cggccagcga
     5041 caattccggc ggctctatat ccagggtgtc tccaactccg gcgacacgat ggcctcgctg
     5101 cgatacatgc gcctgcaagc cctgctgcct ttgctcatcc accgcgagac gccgcgcgcg
     5161 gcgctggtca ttggtctcgg caccggcatt accggcggtg cgctgttgac gtggccgggg
     5221 ctggaacacc gcacggtcgc cgaattgttg ccggccgtcg tacgcgccgc gccgcagttc
     5281 aagggcaact acagcatgag caccgatggc cgcatggaca tccggatgcg tgacggccgg
     5341 cgcgaactat tgcgcaacgc cgaacgctac gatctgatca cactcgaacc gccgccgccg
     5401 tcggccgcgg gggtcgtcaa tctgtactcg tcggacttct atcggctggc ggcgtcgcgc
     5461 ctgcagcaag gcggcatcgt cgcgcaatgg ctgccgttgc cgacgcaaaa tgaacaggac
     5521 acgcgcgcgc tgatccagag cttcatccag gtgtttccgc atgctgcgtt gtggaccacg
     5581 gaattgcacg agatgatgct ggtgggttcg atgcagccgc tcgaactgga cgtgccgcga
     5641 atcgaggcgc gctttgcaca accgcgggtc gccgcggcac tgcgcgaagt cggtatcgcg
     5701 tcgccggcag cgctgctggc cacctggatc actgaccgtg ccggcctcgc gtactacgcc
     5761 gccgacgctc aagcggtcac ggacgaccag cctcgcatcg agtacgcccc gtgggttcgc
     5821 cacgacgcat ttccgacgac gctgactcac ctgctcgcgc tccacaccga gccgccgttg
     5881 cagggcgcgg acgacgcgtt tcgcgccgcc atggagaact cgcgcggcgc attgcacagc
     5941 ttttacgcag ccggactgga tgcttataaa ggcgatcggg atgcgtgggc gcaggacatc
     6001 ggcgacgctt tacgcacgga tccgggcaac gcttactatc gatgggttgt cggagacggc
     6061 aactagccgt cctgagccgc tgtcgagcat ctgccttgcg agacaacccg tagaacgcgg
     6121 agaccccatg aacgacaacc cgccagacgc cgcgaccgag ggtaaaaaat ttctccggga
     6181 aatcttgcgg ccggtcgcgg caatcaaccg atggctggac cagctcgacc ccggcacgca
     6241 ccgtcgaatc aaagggctgc ggctcgtgac ggcgtacggt atcgcggctg cactcgggac
     6301 actgcaggac attgcccaca gcgtgccaag caacgtctcg ctgggttacc ttgccggcag
     6361 cttcgcactc tgggcgagtg tgtccgaagg caaaacgacg cgcgccgagt cgagccggga
     6421 tctcctgctg ctcagtctgg ctgccgggtt aggcgcggcg ctgttcgcgg tttgtttcgg
     6481 gccattgcaa cttgcgggcc gggcaggacc ggaactgatg ctggtgaccg gcgcgtttct
     6541 ggtcggttat ctgaaacggt tcggcgtgct tggcgccggc atcggatcgc aaatctatct
     6601 tggcgagttg ttcgcctaca gcgcccacct gacgacggct gatttgtggg ctatcgcgat
     6661 tgccgcgttg atcgcggcgt tggcgtcgat cgtgccacgc gtgctaagcg gcccggcgga
     6721 gcggcccgct tccctgccag cactcgctgc cggcatggca ccgggcgcag ggagatggtc
     6781 gccggagttc ttcatgggcg tgcaggcggc gctcggcgcg ttggtcatcg tcgttctgaa
     6841 cgggatgatc ggtctggagg aatcggcctg ggcgatcacc gcgtgcacct acgtcgtcgc
     6901 gggctcggcg acgggcacga tacagcgtgt gcgtcgccgg attctcggga cgatggttgg
     6961 cgttccgtta ggtctcgcct gtctgccggt tgcagagcat gcaccgctcc tgatctggat
     7021 tgcagccgca ctcgcgatga tcgtctatgc gatggcgttg ccggagcgct acgacatcgc
     7081 ttgtggtgcg ttcgctttta ccttgatcgt gacgcttgcg gcaagcggcg tgcactcaat
     7141 ccccctgctt gcggcacgcg cctgggagac gcttctcggt ggtgccctcg gtctgcttgc
     7201 cgccatgttt gtttttccgc tcagaacttc cggccccggc aagtagcgtt caaccagcgt
     7261 acgaatgtat ccacgcgccg gtgagccgat gtcgttgacg atcggaccag acagacgatg
     7321 ctccgcggat aaagtttcgt gctgccaatg gaaaacgtcg aaacgcaact gctgcgctgg
     7381 taaagtgccc gtgacttcct cgcttccatt cgccacgctg ccatgccatt catgccaaat
     7441 caaagcagcg cctgcgcctc gatcatgcac cgcacacggc accttccggc catcgctgcg
     7501 ctgagcctgt ccttgcaggg atgtgcgctg cgcggcgcgc cgtcgtacga aatctccggc
     7561 gcctactttc cgctctggtt ggtgagcgcg ctggtcgggt tcgtcggcgc actcattgcg
     7621 catcgaatct tcgtgtccac tgggtgggcg cagatcgtcc cgtttcagct ctccgtctgc
     7681 gtcgcgatcg gcctgacggt cgcggtgctc ttctggcttt cgggaacagg acagctctta
     7741 tgaaaattgc cggcaagcgc caggcttctg tcaaaggccg cgtgatcgcg ggcgtgattg
     7801 ttttacttgg catcgcggcg gccatgtacg gctatgaccg gaccacccgc tttccgtcaa
     7861 cggatgacgc gacgatcgac gccgacgtcg tgcacgtcgc gtcgccggtc ggcggccgga
     7921 tcgtcaggct ggcggtgcag gaaaaccagc gggtcgccaa aggcgacgtg ctcttcgaga
     7981 tcgatcccgt gccgtttcgt ctcgtggtcg cgcagaccca ggccgacctc gaactggccc
     8041 gcgcggcgct cgaaacacgc cgcaggacga ttgtcggcga gcgtgccaat gcaaccatcg
     8101 ccggcgacca gaccggcaag gcggcacaca actacgagct ggcagcacgc accgtgcagc
     8161 ggctcacgcc actcgtggcg aagggttatg tgccggtcca gcagctggat caggcgcagg
     8221 tggccgagcg cgatgccgcg ttgtcgctca agcaggcgca gaagcagaag caggcaagcg
     8281 cgcagacgat cggcgacgag gcggacgccg tcgccgtcgt ccatgcacgc gaggccgcac
     8341 tcgcgctcgc gcagcacagc ctcgacgaca gcgtcgtgcg cgcgccgcag aatggtttcg
     8401 tcacggggct ctcggtgctg gcgggcgaga cggttgcgcc gcatcagtcg atcttcacgc
     8461 tggtgcacgc ggacgaatgg ttcgccgtcg ccaacttccg ggaaggggcg ctcgcgcgga
     8521 tccagccagg cgattgtgcg accgtctatt cgatgatcga ccgaagccag gcgattcacg
     8581 gcaaagtggt cggcatcggg gcgggtgttg ccgattcaga ccgcatcaat ctgccgcgca
     8641 ccttgccgat cgttcagcaa tccgtgaact gggtacgggt tgcccagcgc ttccctgtgc
     8701 gtgtgcggct cgacgagccg gccgcccggc tggtgcggat cggtgcgagc gcgatagtcg
     8761 aggtccggca tggcgcagcc tgccggtgat cttgcccgac cgggccccgc cgacgtcctg
     8821 aagctgcttg cgccctatcc cgggcgagcg gcgatggcgg cccgcatcgc gctgatctgc
     8881 gcgctgacgg cgttcgtgac gagcgcttac ggtacgccgg aagcagccat ctccgcctat
     8941 gtcgtgttct tcctgaaccg gcccgaccgg gtgctgagcg tggtgctggg ctcggcgctg
     9001 ctggtgctgg tcacgctggt cgtcgggctc gtcatggtgg tggccatgtt cgcgctcgac
     9061 gagccgctct ggcgggtggc gtgcatcacg gtgctgtcgt tcggcttcct gttcgtgacg
     9121 tcggcgagca agctgcggcc ggtgggcgcg atcatcgcga tgatcgtcgg ctttgccctc
     9181 gacgaactcg gcatggcgcc gttcggcgaa gcggcgacgc gcgccctgtt ctacgcgtgg
     9241 ttgatggtcg cgattccgat cggcatctcg atcggcgtca atctgttagt ggccccgtcg
     9301 ccgcgcaagc tggcaggccg cgaactggcg aggcgcctgc ggctcgccgc gcgcagcctg
     9361 gccgaacccg aatcgacggg cgacgcgctc agcgcgtgcc tgcgcgaagg ggaagcggac
     9421 ctcggcacat ggctgaagct ctcggtggcc gacggatcgt cgcggcgtgc cgatgtcgcc
     9481 ccgctgcggc aggccgtgtc gtccacgctg gcgatcctcg tcgcggccga tctttcggta
     9541 cacgaaccgt cggcgcggct gccggcatcg ttcgtcgggc cgatcgccgg tacgctggaa
     9601 aacatggcac gcatgctcga ggccggtggc tatccggtcg acatcgaact cactttgcct
     9661 gccgtgacag ggctgacgcc gctcgcgcat atcgtcgtcg agaatctgca cgatgcgatc
     9721 gtccgctttg ccgtggttga aagcgaggtc gcggtggggg ctgaaggcgt gattcaaacc
     9781 gaaggccgtg ctgaaggccg ccctgaagac cgcgccgaag gccgccccga agccggtgcc
     9841 gaaggacgcg ccgaggccga cgccgaagct cgccccgacg cccgtgccga aggacgcgcc
     9901 gaggccgacg ccgaagctcg ccccgaagcc cgtgccgaag gacgcgccga ggccgacgcc
     9961 gaagctcgcc ccgaagcccg ccccgaagcc attaccgcgc cgccggccga cggtaagcgc
    10021 ggcgggttct tccttccgga tgcgttcacc aatcccgatc acgtccgcta cgcactgaag
    10081 accaccgtgg cggcaatggt ctgctatctg ctctattcgc agatcgactg gccgggcatc
    10141 catacctgct tcatcacggt ctacatggtg tcgctcggca ccaccgccga gaccgtcgag
    10201 aagatgacgc tgcggatcgc cgggtgtatg gtcggcgcgc tcctgggtgt cgcggcgatc
    10261 ctattcgtgg tgccggtgtt gtcgtcggtc gtcgggttga tggcgctcgt gctgatgggg
    10321 gcatggctgt cggcatgggt cgctttcggt tcgccgcgga ttgcttatgc cggcttccag
    10381 atcgccctgg tgtttttcat gtgcgtgttg cagggcgcgg cgcctggcta cgatctgacg
    10441 atcgcgcggg atcgcacgat cgggatcctg atcggcaatg tggtcgttta tctggttttc
    10501 actcgcgtgt ggccggtgag cgtggccgag cgcgtcgaca ctgggcttgc cgcgctgcgg
    10561 caacagtggc aacggctggc cgcgctgccc gatgccgtca cgcggcgggc gcaggccgcg
    10621 agtgccatgg cgcagtgcgg cgcgttggaa caggacctcg cgctgatgca ctacgagccg
    10681 tcgtgggttc gtcctgaatc gcagtggatc gccgaacgcc gcagcgcgct ggccaggctc
    10741 gatgcgctcg aaggaccgat gtttctgctt gcagaacgac gcccgggcga tgctgcgatc
    10801 gatggctggc ttgggcgggt cgcgggtggg ttgccgctag cgctagcgcc tgctgaaacg
    10861 attgagccaa acgcagccgc tgacgacagc gcacggaccg ctttggttaa cctcggcgat
    10921 gcacgcctca ctcaacttga acacgctgcg tcgaatgatg cggcgaagga actcacaagt
    10981 catgcgccgg cttaagcctc cattcgcggt ggcgaccctg gtcgcggcgg gtttggccgg
    11041 atgcgccacg tcatccatcg acatggcgcc cgacagcccc gaccgtccgt ggcaaccgca
    11101 gacggacgcg gccggcgcca ttgtgccggg gccggctggg ccgccggggc cagctggttc
    11161 ggctggttcg gctggttcgg ctggttcggc tggttcggct ggttcggctg gttcggccgg
    11221 gtcagctggt tcccccaacc cggccaacac gacggctcca gccaacccaa caccctccgc
    11281 acgcagctac cagttgcccg cgaatcccgc gctcgccgtc gtgccgcctc cgccgccgct
    11341 cgagcaggag cacgcatacg gcctacccga gctgatcgac ctggcggagt cggcgaatcc
    11401 gctgacccgt atcgcctgga acgacgcgcg caatgtcgcg ctcgcggccg gcatcgcgaa
    11461 gagcgcctat cttccgcaac tgtcggtcgc ggcgatgggc cagtacgggg gttttcaagg
    11521 ttcttcaacc tccgtgctcg gcgattcgtc ggtgagcgcg acaggacacg gcaccacctc
    11581 cgtggtcgcg ctgcaatggc tgctgttcga tttcggcgga cgtgccgccc gtgtcgaagc
    11641 cgcgtcgcag gcatcggtga tggcgaacat cggcttcacc gcggtccacc agcaggtgat
    11701 cttcgacacc agtgtcgcgt tctacgcgta ccaggccgca cgggcccgca ttgccaccac
    11761 gcgtctgggc ctcgacaacg ccgaggcgat cctggccgcg gcacagtcgc gctacaagca
    11821 cggcatcggt accgtcatcg aagtggcgca ggccaatcag aatcgcgcgc aggccaggct
    11881 cgcgctcgtc caggcccagg gcgtcgagag caacagctat ctcgcgctga tcgccgcgat
    11941 gggcatttct ccgctgtcga agcccaggat cgcagatgtg ccggcgcaca cgctgccggc
    12001 gtcgacgagc gactcgatcg agcggatcgt gtcgaccgcg atcgcgcggc gcccggatgt
    12061 gttgagcgcc tatgcggccg aacgggtcaa cctggcgaaa gtaaaggcgg ccgaatcgga
    12121 cttcatgccg aaagtattcg tctcggccac gggcgcgtac aacactggca gctcgtcgat
    12181 ttcggcggtt ccggcggtcg gtcagcaatt gccgacggtg aacctgagtg gcggccgtta
    12241 cggcggcagc gtgatcgtcg gcgtgacgat tccgctgtat gacggcggct tgagatcggc
    12301 ggtgctcgca caggcgcgca acgatgccga cagcgcgtcg acccgtctta cccgaagccg
    12361 tgaagaggcg gtccggcaaa tcgtggctgc gcaaaatacg ctgcagacga gccttgccgc
    12421 gcaagctgct gccagggaat tgctggctgc ggcgcagacc acgtatgacg ccgcgttcga
    12481 tgcgtatcga cacggggtcg gctcggtcac cgacgcgctg cttgcgcaga atcaattgct
    12541 cgcggcgaag aacgccgacg cggacagtta cagcagtgcg ttatccgctg cggcagcgct
    12601 cgcgctggcg accgggtcgg tcgattcgat accgatgcgc gacaactagt gaggaagggg
    12661 tttcaacaca tccgcgaagt cgataccgtt cctggccgcg ctcagcaagc atgcgggaat
    12721 aaggtacgcg ccgccgccaa ctcacgcata gcgcgcaaga aacatatcgg cagcggactc
    12781 ggcgacttgc ctttgttcag ccacgctgag gggtggatgg cccatcgtca cctgcggcca
    12841 gaaagcgaac gctttcacca agccctgcaa ctgctgcgag gcgaacatcg ggtccgacgt
    12901 cttgagccgg ccatcggctg cggccgcgcg aatccatatc gtcaagtctt cctcacgctc
    12961 acccatgcgg gtcaccatat cgcgcgcgcg ctccggtgaa tgaatgccgg ccgcgatcgc
    13021 cacgcgcgcg agggagagga acgcctcgtc attcaacagg cccagctttt tcaacagaag
    13081 ctggagcaat tgcgcacgca atggatgcac ggcggaataa accggcgcct cgcccgtatc
    13141 actcgactcc cacaactgcc gcaggatcgc cgcaaacagc acttccttgc ttggaaaatg
    13201 gttgtagacc gtgcgcttcg agacgctcgc gcgcgccgcg atgcgatcca tgctggtcgc
    13261 gtcgaaaccg gccgcgagaa attcttcaat agccgcggca atcacggcag cgcgcttgcg
    13321 gtcggtcagg cgaagagggg tatcacgggt gttcatcgtt aaattttaca ccgggcagtt
    13381 tacttttctc aaaaataaac tacactcaac agtctacttt ctgcatcgag gcggtttgca
    13441 tgacatcgtt caccggttct attcgccgcg ccttgggcct ggcggctgca cgacgcggtc
    13501 gtgaattgtt cgacacgtcg gcccagcacg acggcgagcg ctttcgcaac gtcaagccgc
    13561 gtcctgtaga agggatcggc aagaccttga gcatcgtgtg gaatgtgttg ttcaacaaac
    13621 cggacgacac gaccccggcg ggcgccctgc ccgtcgatgc cctgtcgcgt gaacagctcg
    13681 acgcagcgcc tgagcggagc ctgtaccgcc tcgggcattc gaccatgttg ctcaagctgc
    13741 gtggccagtt ctggctgacc gacccggtct tcgcggaacg cgcatcgccg ttccggcggt
    13801 tcggtccgaa gcgcttccat gcgccgccca ttgcgctcga agacttgccc ccgctacgcg
    13861 ctgtgattct gtcgcacgac cactacgacc atctggaccg cgagaccgtg ctggcgctcg
    13921 ccgccaggac ggccgtgttt ctgacgccgc tgggtgtcgg cgaccgcctg atcgagtggg
    13981 gtatcgaggc tgccaaggta cgccagttcg actggtggca aagcgtggag atcgatggtc
    14041 tcgcgttcac ggcaaccccg gcgcagcatt tctccggccg cagtctgttt gacggcaaca
    14101 gcaccttgtg ggcttcgtgg gtcatcgtcg acgacgcgct gcgcgtgttc ttcagcggcg
    14161 acacgggcta cttcgacggc ttcaagagca tcggcgagcg gctgggtcca ttcgacgtga
    14221 cactgctcga aacgggcgcc tacgatgccc aatggccgta cgtccacatg cagcccgagg
    14281 aaaccgtaca agcgcacgtc gacctgcgcg gccgctggct ggtgccggtt cacaacggca
    14341 cgttcgatct ggcgatgcat cgctggcaag aaccgtatga gcgcgtgatc gggctgacgg
    14401 ccgtgcgcgg cattccgctt gcgacacccc gcatgggtga gcgactcgat cttggcgcgc
    14461 cgcatcgcgg cgagaagtgg tggcgtgcgg ttaaagcgcc tgcgcaggaa cccgtgacgt
    14521 cgcgacgctg gttcgcgtgc cgcaatagtc ggcaagcgaa ttgaggagac tcgctgaaac
    14581 tgaaagcgtg agcaaccggt tgcttgcgcg cgttgctttt cctaacgttc gacctgccct
    14641 gctgcaatgt cctcgagcga ccttccgcgc gttggcacgc ccatgagcac cacggccagt
    14701 gcgccgatca gcagcacaag cgtggtcgca ccgaacaccc cggcaaaacc cagcagcgga
    14761 tagacgtagc cgaccaggat aggcgcggca atcgcgccca gccggccaat cgacgaagcg
    14821 agtcccgtgc ccgttgcgcg cacgtcggtg gggaaaactt ccggtgtgta agcataaacg
    14881 ccggcatagg tgccgttcat gaagaacgac agcaggatgc ccgacaccat gatgccggca
    14941 tccgtacccg tcaacgcaag cccaagcgcc gatacgccgc ccaggatcat gtatgacgcg
    15001 atggtcgcct gccggccgat cttctcgttg agccacgcgc ctgaaaagta ccccgggatc
    15061 tgcgcgatat aaatcacgag cgaatacgaa aagctccgcg tgatcgtcat tccgttctga
    15121 accagcaggc cgggaatcca cgtgaaaaaa gcgtaatagc tgaacgtgat ggatagccac
    15181 atcagccacg tcatggccgt gatgcgcgcg agcttcgccg cccacagcgc cttcacgttc
    15241 gcgaggatgg aggcccgtcc ggctgcgagc gggacatgtt cgtcgcgtac ggcaggcagc
    15301 ggctccaact gcacgccccc tttgatcacc tccgcttcca tgcgcgacac gatcgcgtcg
    15361 gcttccgcgt gccggtcgcg cgcctcgagc cagcgcggcg attcgggcaa cgcgcggcgc
    15421 caccagagca gcatcacaat gggcagcgca gtgatgacca tgaccgcgcg ccatgcattg
    15481 ggggcgagcg gaatcaccag atagccgagc agcgccgccg ccacgaaacc aaaagagaaa
    15541 aaccccgcga gactgcccgt gaaactcccg cgataccgtc gcgccacgaa ctccgagaga
    15601 aacggcgcga cgatggcgct ttccgcgccc gttcccaacc cggcgacaat gcgcgtcgcg
    15661 agaaaaaatc cccagtcatt cgctaccgcg ctggcaagag acgctatgca gtaaatcaca
    15721 agcgcataca tcatgacctt gcgccggccg atgacgtcgc cgagcatgcc cgcaagcatc
    15781 gcgccgacga aatagcccat gaacgtgccg cttcccagca cgcccgtctg cacactcgtc
    15841 aacccccatt gcgtgcgcag caccggcagc aggaacgcga gcacggcagc gtccattgcg
    15901 tcgaaggtgt agccgaggcc gcccatcagc agcagacggc ggtgaaaacc tgaaaacggc
    15961 agacgctcga tgcgggcggc aatc
//
LOCUS       DATASH010000337        17979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5784490,
            whole genome shotgun sequence.
ACCESSION   DATASH010000337 DATASH010000000
VERSION     DATASH010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 17979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17979
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5784490"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="VHV99_09410"
     CDS             <1..760
                     /locus_tag="VHV99_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3635690.1"
                     /translation="AFILARAGLLDQRMVTTHWSYADELAALCPTARVEVDLPFVEDG
                     NLCTSGGITAAVDLSLSLLKLDKGNEVALKVAERLVVSMQRAGVKSQSSPHFTPYAQG
                     SSWVAQVQQYVLSNLTGDLSVKDLARVTNMSMRTFARTFVRDAKISPAEYVVGARVDA
                     ARVMLQGTAIPLKTIAYECGFGNADRMRCVFQRCMGVSPKQYRLHHQSETCLDRELYA
                     VGCHVQVPERQNSRSPQIPSTLEYQSIASGKKTV"
     gene            complement(731..928)
                     /locus_tag="VHV99_09415"
                     /pseudo
     CDS             complement(731..928)
                     /locus_tag="VHV99_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611899.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1289..1642
                     /locus_tag="VHV99_09420"
     CDS             1289..1642
                     /locus_tag="VHV99_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX3635691.1"
                     /translation="MKACNVVNLLVVAGCVPLTSNVYAQASNAMPMASSPAASSRKAT
                     PADKQLAHDVRKALSKAPNFNVSNVFVKVRGGVVTLSGSVPDGPQIQQAAEVAKDVPG
                     VTSVSNHLSLYTKGY"
     gene            2348..2887
                     /locus_tag="VHV99_09425"
     CDS             2348..2887
                     /locus_tag="VHV99_09425"
                     /inference="COORDINATES: protein motif:HMM:NF013578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="jacalin-like lectin"
                     /protein_id="HEX3635692.1"
                     /translation="MTVASTDCLGRQKMTGVLKCALLMFAHCAGCLLVLEGSLAQAQV
                     LIQGPSGGDGGSSLDACANYTVKSVTITSGQYINSISVDYDDQACCHRLAGSTARDSV
                     TLPVPPGDRIIGVSGRYGKYVDHIKIWTAQNPVGVEAGGPGGAVDFEYHALPGTAIIG
                     FMGRAGQYIDAVGVCMRSQ"
     gene            3578..4252
                     /locus_tag="VHV99_09430"
     CDS             3578..4252
                     /locus_tag="VHV99_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635693.1"
                     /translation="MKKMTASLIAFVFLFTYTAAIAERPDSHESLQNTSRTSDVRDFF
                     TPAQWTSDARSEFSARRVQSPDNNRAIAQVSLCSADETVFFSCAVKNGEKVVSLCGTK
                     GLSRNQGYLQYRFGRSSHIELEYPKAREDTRRAFSYAHYFRALVDLSEISFTIDKYRY
                     SIFDHADSESKPATREAGVRVTASNDDSTTTLVCRGAPTSRLAALANVLPCDKESGLN
                     ISGCTQ"
     gene            4265..5242
                     /locus_tag="VHV99_09435"
     CDS             4265..5242
                     /locus_tag="VHV99_09435"
                     /inference="COORDINATES: protein motif:HMM:NF023288.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramidase family protein"
                     /protein_id="HEX3635694.1"
                     /translation="MSPTCESFIRAVLTQSWRDAYLNLNGLNMDEMLRAQAALDPLDL
                     ADMWAQRQAFTGQIDMPRIEYAHDVVTTRKLPQTAPGDLASTGQTNDAKSFIGKPRPL
                     TFEHDLTGTLPATSAAPPQLGEHDYVLAAKQNNVEVAAIMAVAQVESGGRSGFAADSK
                     PIIRYELHLFDRHTGKAYRKTHPHLSQPNLASGVPYHHGGQTNEWSLVFGAMILRDSK
                     GGRRIAEAWQSTSWGMFQVLGSNSRMVGWPSVGAFVADMFVSEAQHLRAFLGYCKATH
                     LLPQIRTRHWAAFARGYNGPTYAVNNYDSNLANAFASISNRRHGSRLPP"
     gene            5888..6703
                     /locus_tag="VHV99_09440"
     CDS             5888..6703
                     /locus_tag="VHV99_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635695.1"
                     /translation="MANPAERKGQSPDFRAQKLYAQAKTAVGQTGWGAFRGQSWMEAG
                     TALSMSALGKSTITNGVQSMATSGMATAAASVAVTGISWCFPLLRPIVSAAAHACETM
                     SWAVENTGPVGEAAVGMVSEKIEELIWGAVAPDGMDSVNTAADTANIDSKELLENLKE
                     QLVQIVATMNVATKFEQRSATYCDELYRYALMVESMKDNIVEARKNAKTLHKFLGKVV
                     KILPDDAAILQMETSAKKAIAAKLDSGTMKHYNGAWISGRHAMCSNVHCWGAA"
     gene            complement(7089..7532)
                     /locus_tag="VHV99_09445"
     CDS             complement(7089..7532)
                     /locus_tag="VHV99_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007584226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX3635696.1"
                     /translation="MTISGVAPGEPINIGQLSIQYLIDGTATGGMGVFELTVPPSSQV
                     PPPHSHAHNEECVYVLEGMLRYSVDGVVRDLTPGEWMFTPRGSVHHFSNPHNGTARAL
                     IVMTPDIGAQYFRDVGTIVNAGGPPDREKLIGVMSKYGLVPAPPQ"
     gene            complement(7785..8567)
                     /locus_tag="VHV99_09450"
     CDS             complement(7785..8567)
                     /locus_tag="VHV99_09450"
                     /inference="COORDINATES: protein motif:HMM:NF022628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2968 domain-containing protein"
                     /protein_id="HEX3635697.1"
                     /translation="MRHTPTKPLRFAALTLALTAVQMLSGAHASEAAAVPGASDATVQ
                     VPLGPSVSSAPASLSATTASSGAQPGGDVSTLTQLVQQGRVTPLRSTVAGNYSATLSF
                     FGDDLTYYVALSQQSTYWRVIATQNKKRAEAVYADFVVQTERLAAAQNRRAELAAQEV
                     ATQRLIEQAQIKAQQLQADAQIAQTQRDEVATQQAQAADLVKNLKAEDQDARKYLSQI
                     RRQIVGLQIASNSGLPPEHPARLNKTRKHAQHTRIAALATTQ"
     gene            complement(8631..9653)
                     /locus_tag="VHV99_09455"
     CDS             complement(8631..9653)
                     /locus_tag="VHV99_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX3635698.1"
                     /translation="MLRFRPAEFALDVGTANTRIHIAGAGLVLSQASVLCTHGRDSLR
                     AGSRPTVSVGDEARRMLGRLPQNIEAITPIRGGVISNFPASEQMIRQFVHHARKDQRL
                     TNAPRITVTVPGGATQVERRAFKEVVHGAGASHVALLARPLAAALGAGLAISEATGCM
                     VVDVGAGTTEIGVIALGSVVRGASARVGGDTLDQAIVNYVRRTHGLLIGEHTAQRVKL
                     DIGSALPPKDDRFTGVTGRSLAEGVPRSMTLSGQEVYEAIIEPLEQIVSLLRRVLEST
                     PPELAADIADRGFMLTGGSAMLRGLDQRLREETGLPVAVAEQPMTCVIRGTGLAIETL
                     DSHFFA"
     gene            9540..10028
                     /locus_tag="VHV99_09460"
     CDS             9540..10028
                     /locus_tag="VHV99_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635699.1"
                     /translation="MSTQHRCLTQDQAGPGDVNSRVCRSYVEGELSRAKAQQAPYRHE
                     KCVAVTRSSILDFADELLCGYVCVRNHFRMERKSCVDASVIGQGSKFFNLHEGSRLTT
                     NFASACSRARSGLSRRIHDCPLNRPQPPLVAATADFSSTFDVAVRDHISWRREHSPEL
                     PH"
     gene            10265..12175
                     /locus_tag="VHV99_09465"
     CDS             10265..12175
                     /locus_tag="VHV99_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflK"
                     /protein_id="HEX3635700.1"
                     /translation="MPNRRAGEEIDLSDRPSGRSEASLAGWMALGLAGLLGILSLLKT
                     MPVDLRWSAVLASACLNVFVVTIWRPPRPRTPRDPAQGSAWKQGVSFIRRLVWQDAEA
                     PPLSLAVSGFHWQPWGVAIGNCLSVAIAATALQPLPPALIHVMVTSSLVIVSGAAALT
                     LAFLTLVAERFYAQDAEQSHASLSASIAGMLRVLVVCALAAALSAAWLVYQHTALDWL
                     VHVAAVFTATIAVEMMIRATLLRFFAPRPGRNPVRVPSSIIAGMLRSRGSSLSSIGAE
                     LHERYGIDLRQNWVLRSFVRLLPVTVVAMAACAWLLTSVTVLGPSQRAVYERFGAPVA
                     VWPPGVHFGMPWPFGKARLVDNGAVHQIIVSGGADDSSVAAPATPADGPTSEQMNRLW
                     DVQHPWDTSQVIAGATGEQQSFQIVNADVRLDYREGLSDAAARAALYRSVDPESTVRS
                     IANREVVHYLASHTLQALLETSQTAMAESVRGAVQRQLDQLSSGIEVVAVVIESVHPP
                     SGAAAAYHDVQAAQIRAQASVAEAHGFAAGLLGDAQQQAQASVTQASARAADTMAAAR
                     VQQINFEADTVAYRLGGPAFPFEYYLSKLQKGLQNARITVIDDRLTADRRATIDLRSF
                     TAGDLTGIRHGN"
     gene            12251..13312
                     /locus_tag="VHV99_09470"
     CDS             12251..13312
                     /locus_tag="VHV99_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HEX3635701.1"
                     /translation="MSLFHSHADGHDHGHRHHHHDGGAAQGARGGPFYGRVLLALLCV
                     AVAIAVASFISVRAGEASVITRFGQPVRVLLEPGLAWRLPAPIEAVVPVDLRLHTTSS
                     GLQDVGTRDGLRIIVQAYVAWRVAADPNDVRRFVRAVGNQPDEAAQQIRSLLGSSLQT
                     ASAGFDLASLVNTDPSKVRIAAFEDALRSQIDAQLYSAYGVHVVQVGLERLTLPAVTL
                     AATVSRMSAERETVAAQRTAEGNREAAQIRSDADRDARLAVADANVKAAQIEAQSRKD
                     AAEIYGATYAGNPRLYTMLRSLDTLDSVVSTNTNLILRTDAAPFQALVQGPTALSPES
                     LPQPGAGSASGSKKIRSAR"
     gene            13309..14478
                     /locus_tag="VHV99_09475"
     CDS             13309..14478
                     /locus_tag="VHV99_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX3635702.1"
                     /translation="MSTDTGVPPLGPGAQAVRLSFWFVAALVVLAACAWAGSNIRRIP
                     PDSRAVVLRFGSFVRTQDAGLLIAWPRPFESTLLVPGSARVLEQRIQSLERDPRARAA
                     DMNLPEAAPSAQGPAGTGAVIGPEDGPPGPPPAPDNAADPDAAPPVAQLPDALAGSGY
                     VLTGDNGVLQLRATLYYRVVDPYAYVLQEDRLAAALERIVSASAVSVVAQRDLDAILV
                     ARPELLSADQQVAIKRERLRGDLAQEVERHLRELEAQHAGLGVEVARVDVEVAFPAAA
                     VSAFNSVLTSLQVAERSMAEARTSAEKTRQDAQQDADRIVQNAQASAVERVATAQAKT
                     MTIQQLEVPLRTNSDPGLVVRLYRDRVQSILSKAGSVTTVGPNDTSNLILPGNTR"
     gene            14475..16481
                     /locus_tag="VHV99_09480"
     CDS             14475..16481
                     /locus_tag="VHV99_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEX3635703.1"
                     /translation="MSTRVEHTTHPGGHVHTHTPHADEANGVAQGRAIVLSASEQRGV
                     TRQVALALLAGGLLILSTAWHTFAPADEALAQILAGLASLVVAGPVFAGAWHSLKSPS
                     LHGVTDRLIALAMLAAWAVGDMATAALLPIVMTFGHALEERSVLGSQEAIRALGRLTS
                     GKVRRVKADGSIEEVQYDAVRAGDHIEVVAGARIPADGVVQHGDSSVDNGPITGESVP
                     LDVEPGSTVFGGAMNLDGPLRIEVTHAGKQSTLGKIIELMQRAEQAKPPITQVLERYM
                     GGYLCLVLLIAAIVWFVSANASAMLAVIVAACPCALVLAAPSTAIAGIAVGARHGILF
                     RSAAFLDKFAQIDSLVIDKTGTLTYGELRVEDVMLEPGADAAQVLELAARLGGSSAHP
                     VSRALLRYATDAAPAGFERAPFEHTRELRGLGVLAQTPEGVAVLGRYGLLADYVQALP
                     EPRREIEGPCVGLALNGRLLAWFGFADALRTEAKDALAELRELGLTRQTLLTGDREPV
                     ARKLAAETGIAVVVAQALPHDKLDYVVREMAAGYHPLVVGDGLNDVLAIKAGPTSIAM
                     GERGIDIAVASADVVLLGDDLRRIPTCVRLGRRCLRTAAINAAIGLGWTVAVIALAAT
                     GVLSAVWIALLHNLGTFIVIANAGRLLRMDEGLPLPTPIREGNT"
     gene            complement(16634..17029)
                     /locus_tag="VHV99_09485"
     CDS             complement(16634..17029)
                     /locus_tag="VHV99_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009771090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX3635704.1"
                     /translation="MLILGVMLVGLLMGAARGSAQDAPRTFTISPGYRFTEKSGGALY
                     NATCAGCHMPDGKGAQGAGHYPALADNPAVEAAPYVIVNVLHGRKAMPSFGDAMSDDQ
                     VADVVNYTRTHFGNRFAGSVTPSEVRSLR"
     gene            complement(17034..>17979)
                     /locus_tag="VHV99_09490"
     CDS             complement(17034..>17979)
                     /locus_tag="VHV99_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3635705.1"
                     /translation="DPDGAYQAGRRSSERRGFATAPGGGLMPRAVPSNLLPRDALLAS
                     NLWQWLSSGSDQDFQSSIFQPVGGMDRIAHALYSQVASAVVFNARVTAILQDEQGVTV
                     RYTDTTTRSERNAHADWLVCTIPLSILSQIDIAVGPAMRAAIDAVPYETSVKIGLQFG
                     RRFWEEDEQIYGGITHTDLPISMIGYPATGYGSTGPAVLLGAYMWGPASYEMGALPPT
                     ERIAVALSQGSQIHPQYAREYQNGFAMSWSRSPFTNGCFAAWTDALRTAHYANLCQID
                     GRIVLAGEHASHIPAWQEGAVLSSLDAIARLHRRIVNG"
BASE COUNT         3386 a         5333 c         5620 g         3640 t
ORIGIN      
        1 cgccttcatt ctggcgcgcg ccggcctgct ggatcaacga atggtcacca ctcactggag
       61 ctatgcagac gagctcgcag cgttgtgtcc gaccgcccgt gtcgaagtcg acctgccttt
      121 tgttgaggac ggcaacctct gtacgtcggg cggcataacg gcagcagtcg atctctccct
      181 ttccctgctg aaacttgaca aaggcaatga agtcgcgttg aaagttgccg agcggctcgt
      241 cgtatccatg cagcgcgcag gtgtgaaatc ccagtcgagt ccccatttca caccatatgc
      301 gcaaggcagc tcgtgggttg cacaagtcca gcaatacgta ctgtcgaatc tgaccggtga
      361 cctgtcggtc aaagatctcg cacgcgtaac gaatatgagc atgcgtacat tcgcccgcac
      421 gttcgtacgg gacgcgaaaa tctcgcctgc cgaatatgtc gtgggggccc gcgtggacgc
      481 agcgcgcgta atgctgcagg gtaccgcgat tcccttgaaa accatcgctt atgagtgtgg
      541 ctttgggaat gccgatcgca tgcgatgcgt cttccaaaga tgcatgggcg tctcgcctaa
      601 gcagtatcgt cttcaccatc aaagcgaaac gtgccttgat cgggagttgt acgcagttgg
      661 ctgtcacgtt caggtgcctg aacgacagaa tagccggtca ccgcaaatac cttccactct
      721 cgagtatcaa tcaatagcct ctggcaaaaa gaccgtttaa aaaggactgc tccgtatgtc
      781 tgaagctgtt ccggaagacg agggagcaac ccagtttgag gaatgcttcg tgaatgtcgg
      841 cgcgacgctc aaagcgaatg cgaagacgat gaaagttgtg gcggcatgca tgagtacgct
      901 cggccaccca gcggaatttg ccgaggcctt cactgcatcg attcgtaaaa tcgtttcatc
      961 catagtcgac gcttgcgtct tacgctatcc tggatagccg acagctctac gcagttttcg
     1021 taggcccatg catccattac cttcccaaca actcgttgtc cgcgagcgtc tgtgagatcg
     1081 aattgatcgc gcgcgtccgg ctctgccgtg aaggtctcgc ccgcgatact gcggctactc
     1141 attcgggaac gcgcccgacg atcggcctga aagcggacgc gagcgtctga atgtgggttt
     1201 tcccttaatc gagcaagcgg ctttgttaga agcggaattt tggctatctt tgactcacga
     1261 tctgcgcggc cgaacaagag aggcaatcat gaaagcatgc aacgtcgtga atctgctcgt
     1321 cgtcgcggga tgcgtgccgc tgacgtcgaa cgtctacgcg caagccagca acgccatgcc
     1381 gatggcgtcg agtccggctg caagctcacg caaagcgacg ccagcggaca agcagctggc
     1441 ccacgatgta cgcaaggcgt tatcgaaagc acccaacttc aacgtgtcga acgtattcgt
     1501 caaggtgcgc ggcggtgtcg tgacattatc cggcagcgtg ccggacggtc ctcagatcca
     1561 acaagccgcg gaagtggcga aggacgtgcc gggtgtaacg tcggtgagta accatctgtc
     1621 gctctatacg aaagggtact aacccttcaa ggcgccgccg ggatgtactg gccgttttca
     1681 gccgtgaata ttccaggagg agccgacgcg ccgtgcaatt tcaagcgctg ctttagcgcc
     1741 ttcgacttgc aatagcgtcg gcaaccttac gccgttcttc gccgctgtcg cttcggcgag
     1801 aatacgtctg atcccctcca tatctcatcc ccggaaatta tgtgaccacg ctcacagccg
     1861 cggtgcaagc gattatgcgt taggagtttt actgaactag taaacatagc aacttcaggc
     1921 cgaccgaacg gaacgcaatt catccaggat tgtgcgcctc tcaggaatct attttgtccg
     1981 ggagcggcga caggcctcaa taggtaatca gcgcacccga atcctgttcg agcggaccgg
     2041 tcctagtcgg cggagtctgg ccgacgcgac cgcggatggg gcacctctcc gctgcacccg
     2101 ggagattgct ttgcacgtcg cgaagtcacc caagcgggtt gcccctatcc gagtggcgcc
     2161 gcctgtgcgg tcaatcgcct gacgctttgc gcggccacgt caattatctg ccgagcgcaa
     2221 gccgaccagc ggctgtccga ccgatggggt gttttgtctg agcgcttgct gctcggcgag
     2281 taagttatcg attttgacgt gcaaccggcc tgactactgg atcacggccg tttcgtggca
     2341 tgcgcgcatg accgtcgctt cgactgattg ccttgggaga caaaaaatga ctggagtact
     2401 gaagtgcgcg ctattgatgt ttgcccattg cgcaggctgc ctgctggtcc tggagggcag
     2461 tctcgcgcag gcacaggtgc taatccaagg tccttctggc ggtgacggag gctcaagttt
     2521 ggacgcctgc gctaactaca ctgtcaaatc ggtcacgatc acgtcagggc aatatatcaa
     2581 ttcaatatcg gtagactacg acgatcaggc atgctgtcac cggctggcgg gttccactgc
     2641 tcgcgattca gtcacacttc ctgtaccgcc gggcgatcga atcatcggcg tcagcggacg
     2701 ctacggcaag tatgtggatc acatcaaaat ctggacagct caaaatcctg taggtgtcga
     2761 agctggagga cctggcggag cggttgattt tgaataccac gctctgccgg ggactgccat
     2821 tattggcttc atgggacgcg caggtcagta tatcgacgcg gttggcgtat gtatgagaag
     2881 tcaataactg cgctttttga caccatcact aagccgcccg ggaaagggag tcacgctatc
     2941 tccacatctg ctacttgtct gacggtcgcc acgatgaacg cgattgtcag cggatgcgtg
     3001 ctttcggacc cactcaggcc gccggaccgg gcgtcgcctc aacggcagct tccagggttt
     3061 tgaagccatc cgacgctgat gtctagatgg ttgattctct ggccacttcc tatggattgc
     3121 atacaacaag attgaagggg attgacgggg cgaagcgcgc cgcatgtctg ccatgaacag
     3181 cgccggtgcc aagcagcaaa gcgttcagca agttgaagtc gaaagtcgaa aaggctgtga
     3241 tcgattttcg gaatctgttc gagcgcggcg gtaccgccgc cattctgctg gttagcgggt
     3301 tgagcctgca gcgagtgttg aaaatcgcgc gtcaccgttc gtcttacata ggtacgggag
     3361 agtgcgcgag atcgcctttc gagcggggat cggcccaatg ctgtcctgtt ggccaccgca
     3421 cgatacgatc acgagttgga ggaacagaac atcccggaag cgagcccttg tgctgcggag
     3481 gggtggcaca agagtggtga aaaaaagaaa tcgctcgatc accagcgtcg ttttttgtgg
     3541 tcgcgccccc gccacgaaag aagctcagga gatagcattg aagaaaatga ctgccagcct
     3601 gatcgctttt gtttttctat ttacttacac ggccgctatc gccgagcgcc cggatagtca
     3661 cgaatcgctg caaaatacca gccgcacttc agacgtcagg gattttttta cgccggcgca
     3721 gtggacttcg gacgcgcgct ccgagttctc cgctcgaagg gtacagtcgc cggacaacaa
     3781 ccgcgccatt gcacaggtgt cgctttgctc ggcagacgaa acggtgttct tcagttgcgc
     3841 ggtcaagaac ggcgaaaagg tagtttctct ttgcggcacg aaaggactca gccgcaacca
     3901 gggatacctg caatacaggt tcggcaggtc cagtcatatc gaactggaat atcccaaggc
     3961 gcgtgaggac actcgacgcg cgttttccta cgctcactac tttcgagcgc tcgtggacct
     4021 ttcagaaatc agcttcacga tcgacaaata ccgctattcg atctttgacc atgctgactc
     4081 tgagtcaaaa cctgcaacac gcgaagctgg tgtacgagtc acagcatcga atgacgattc
     4141 aactacgacg ctggtttgtc gcggcgcgcc cacctctcgc ctggcggcgc tggcgaatgt
     4201 tcttccttgc gacaaagaaa gcggcttgaa tataagcgga tgcacacagt agatgaggtt
     4261 gcacatgagt cccacctgcg aaagcttcat ccgggcggtc cttacgcaaa gctggcgtga
     4321 cgcctatctc aatctgaatg ggctcaacat ggacgagatg ctccgcgcac aggccgcgct
     4381 ggatccgctc gacctcgctg acatgtgggc acagcggcag gccttcacag gccagataga
     4441 catgccccgg attgagtatg cgcatgatgt cgtcaccacc cgaaaattac cccagacggc
     4501 acccggtgat ctcgcgtcga cgggccagac caacgacgca aagagcttca tcgggaaacc
     4561 aaggccactc acttttgagc atgatctaac cgggaccttg cctgctacca gtgccgcccc
     4621 gccgcagttg ggcgagcacg actatgtcct ggcggccaag cagaacaacg tagaagtggc
     4681 cgcgatcatg gcagtggcgc aggtggaatc gggcggacgg tcaggcttcg cagccgacag
     4741 caaaccgatc atcagatacg aactccatct gttcgatcgc cacacgggca aagcgtaccg
     4801 gaaaactcac cctcatctgt cacagcccaa cctggcgtct ggtgtgccgt atcatcatgg
     4861 ggggcagacg aatgagtgga gcctggtctt tggggcgatg atcctacgcg acagtaaagg
     4921 cggacggcga atagcggagg catggcaatc gacttcctgg gggatgttcc aggtcttggg
     4981 gtcaaattcc cggatggtcg gatggccaag tgtcggggcc ttcgtcgcag acatgttcgt
     5041 ctccgaggcc caacacttgc gagctttcct cggatactgc aaggcaaccc atctgcttcc
     5101 ccaaattcgg acacgtcatt gggctgcttt cgccaggggt tacaacggac caacctatgc
     5161 cgtgaataac tatgactcga acctggctaa cgcgtttgct tcgatcagta accgccgcca
     5221 tggctcgcgg ctaccgccat aattcttgca aggtcggaca gccctgtcac cagggcgctg
     5281 tcacgttgcg aacgaaggtt tgttgtctgg gcggaaccga cgttcgaacg tccatgtgaa
     5341 agcgtgggcc aacatccgca gttggccggt cacgggccga tgctcgatgg gcaaatgagc
     5401 cgccgttgct ccgcctccgt ccggctcagt tcgacccaca gctgtcgttc gaactcgcgt
     5461 cagcgcgatg actgcttcca aggtgcaagc gccgctcgcc acgtcgagcg agttgaaggc
     5521 tcatcggact ttgcggtcaa tcgtgattga cttatcgccc ggatcgggca atcaccactt
     5581 tcgagctgcc gctggctgac gcaattgatt ctttgctgag attcaacctc gtatccacag
     5641 gtgaagtgcg agacccgcaa gcctcattca tgcattcgac gaaagtcgaa ttttttcctc
     5701 ggtattcaag ttatttggcg cagtgccgat acaccgtatg ccttcacctg tagccccact
     5761 gtttgcaggc ttgttcgatc agagggagct tgagctagaa gaggttccat cgctattaaa
     5821 cgattacacg gcggattcgt cgaattcgag ttttaactga ttctaaaaat taggaaggaa
     5881 agttaccttg gcaaacccgg cagagcgtaa aggtcaaagc cccgatttcc gcgcccagaa
     5941 gttgtatgca caggcgaaga ccgcggtggg gcagacaggg tggggtgcat ttcgcggcca
     6001 gagctggatg gaggccggga cggcgttgag catgtcagcg ctggggaagt cgacgatcac
     6061 gaacggtgtc cagtcgatgg ctacctcggg catggccacc gctgctgcca gtgtcgccgt
     6121 cacgggtatt tcatggtgtt ttccgctgct tcgtcccatt gtgtccgccg ccgcccacgc
     6181 ttgtgaaacg atgagctggg cggtagagaa tacggggccg gtgggcgaag cggccgtggg
     6241 tatggtgtcg gagaagatcg aggagttaat ttggggggcg gtggctccgg atgggatgga
     6301 cagcgtgaat accgcagcag atactgccaa tatcgactcg aaggagttgc tggagaattt
     6361 gaaggagcaa ctggtccaga tcgtggccac gatgaatgtc gccaccaaat tcgagcagcg
     6421 gagcgctacc tattgcgacg aactctatcg ctatgcgctt atggtcgagt ccatgaagga
     6481 caatatcgtg gaggcgcgta aaaatgccaa aacgctgcac aagttcctcg gcaaggttgt
     6541 aaaaatcctg cctgatgatg ccgcgattct gcagatggaa acttccgcta aaaaagccat
     6601 cgcggccaaa ctcgacagcg gcacgatgaa gcattacaac ggcgcctgga ttagcgggcg
     6661 ccacgccatg tgcagcaacg tgcattgctg gggggcagcc tgacggctgt gctcggacct
     6721 gggctctgtc gcaatgtgtt cagcagacgc ggcagtcgcc ttcgcgccaa gcacctcacg
     6781 accgaccgtt caagtgaatc tcagcgactc gaagggcaac gcgcttgggg gaaatgaaat
     6841 aaagcggtct tactgcatcg cgtggcggat aagcgcttcg cggtacgcaa gtgcgtccgc
     6901 aatctgggca gagccgtcag ttgaatgtcc ggctaggcgc gcgagcgaat ggccgaggtt
     6961 ggctgcgtgc tgcatcatgc aaagggcata cgcaaagtga accgcgcaag tctgtgatcg
     7021 gcagcacgcg acggcggatt caaccggtgg atgcaatccc ctgcaggcaa tcgttgggtg
     7081 accgctgtct attgtggcgg cgccggcacg agtccgtact tagacatgac accgataagc
     7141 ttctcgcggt cgggcggccc gccggcattt acgattgttc cgacatcacg aaaatattgg
     7201 gcaccaatgt cgggcgtcat gacgatcagc gcgcgggccg tgccattgtg cgggttgcta
     7261 aagtgatgga ccgatccgcg tggggtaaac atccattctc ccggcgttag gtctcggact
     7321 accccgtcga ccgaataccg aagcatgccc tccagcacat agacgcactc ttcgttatgt
     7381 gcgtggctgt gtggcggcgg aacttgcgag ctggggggca cagtcaattc aaaaacgccc
     7441 atcccgcctg tggcagttcc atctatcagg tactgaatgc tcaattgacc aatgttgatt
     7501 ggttcgcctg gcgccacgcc gctgatagtc attctcaaac tccttcagat tgaggattct
     7561 cgtcgttccg ccaacgttgc cggtagggag cgcaattcaa tcaagattat gcgcctctca
     7621 ggaacgtatt ttgtacgaga gcggacatcc cttgttccag gtgaatcccg tttccgcgtc
     7681 cgcccgtgca ttgcgcaaat agggcggcag tttgcagtgc agcagccacg cggttctgtc
     7741 gcaatgaggc gaatcttcaa gctcgtccgg agaatccgca tggctcactg cgtcgtcgcc
     7801 aacgcggcga ttctggtgtg ttgagcgtgc tttctggtct tgttcaatcg cgccggatgt
     7861 tccggcggaa gtcccgaatt ggacgcgatc tgcagaccaa caatttgacg cctgatttgc
     7921 gagagatact tgcgcgcgtc ctgatcttcg gccttcaggt tcttgaccag atccgcggcc
     7981 tgcgcttgct gtgtggcgac ttcgtctcgc tgcgtctgag cgatctgggc gtcggcctgg
     8041 agctgttgtg ccttgatttg cgcctgctcg atcaggcgct gggtcgctac ttcctgcgct
     8101 gccagctccg cacgcctgtt ttgcgcggcc gccaggcgtt cggtctgcac cacaaagtcg
     8161 gcgtacaccg cttcagcccg tttcttgttc tgcgttgcga ttacccgcca gtaagtgctt
     8221 tgctgcgaaa gcgcgacgta gtaggttagg tcgtcaccaa aaaatgacag cgttgcgctg
     8281 tagttgccag ccaccgtcga gcgaagcggc gtcacacgtc cctgttggac aagttgcgtg
     8341 agtgtcgaaa cgtcaccgcc gggttgcgcg ccagacgatg cggtcgtcgc agacagactt
     8401 gcgggagctg agctgactga cggtcctaat ggcacctgta cagttgcatc ggacgcgccc
     8461 ggaacagctg cggcttcgct agcatgggcg ccggacagca tctggactgc ggtcaacgcc
     8521 aatgtcaatg cggcgaagcg gagcggtttg gttggtgtgt gtcgcatggc tgtgtattga
     8581 ttcagagata cgctgaagga aattcgaggc gatgtgtgtt ggtgtgtccg ctacgcaaag
     8641 aagtgagaat cgagcgtctc gatcgcgagc ccggtgccgc gaataacgca cgtcatcggt
     8701 tgttcagcga ccgccacggg cagtcccgtt tcctcgcgca ggcgctgatc gaggcctcgc
     8761 agcatggcgc tcccgccggt cagcatgaac ccgcgatcgg cgatgtcggc tgcaagctcc
     8821 ggcggggtgc tctccaggac gcgtcttagc agcgacacga tttgctcaag cggctcgatg
     8881 atcgcttcat atacctcttg gcccgatagg gtcatcgagc gcgggacgcc ttcggccagg
     8941 ctgcggccgg tcacccccgt aaaccggtcg tctttgggtg gaagtgcgct cccgatgtcc
     9001 agcttgaccc gctgcgcggt gtgctcgccg attagcaagc cgtgtgtgcg gcgaacgtag
     9061 ttaacaatgg cctggtcaag cgtgtcgcct cccacgcggg cggatgcccc acgcacgacg
     9121 ctgcccagcg caatgacgcc gatctcggtc gtgccggcgc caacgtcgac gaccatgcaa
     9181 cctgtcgctt cggagatggc gagtccggcg ccgagggctg cggccagcgg tctcgcgagc
     9241 agcgcaacgt gggacgcacc ggcaccatgt accacttcct taaaggcgcg gcgctccacc
     9301 tgggtcgcgc cgcccgggac ggtgacggtg atgcgtggcg cgttcgtcag ccgttggtct
     9361 ttgcgggcgt gatgcacgaa ttgccggatc atctgctcgc ttgccgggaa gttcgagatg
     9421 acgccgcccc ggattggcgt gatggcttcg atgttctgcg gcagcctgcc gagcattctc
     9481 ctggcctcgt cacccacgga cactgtcgga cggctgcccg ctcttaacga gtcgcgtcca
     9541 tgagtacaca gcacagatgc ctgactcagg accaagccgg ccccggcgat gtgaattcgc
     9601 gtgtttgccg ttcctacgtc gagggcgaac tcagccgggc gaaagcgcaa caagcgcctt
     9661 atcgacatga aaagtgcgta gctgtaacca gatcatccat tcttgacttc gccgatgagt
     9721 tgttatgtgg ttatgtgtgc gtacgcaacc attttcgaat ggagcgaaaa tcttgcgttg
     9781 acgcctcggt tatcggccaa ggttcaaaat tctttaattt gcacgaaggc tcgcggctta
     9841 cgacgaattt tgcgagcgcg tgcagtcggg caagaagcgg cctctcaaga cgtattcatg
     9901 actgcccgct caacaggcca cagccgccgt tagttgccgc caccgcggat ttcagttcga
     9961 ccttcgatgt agccgttcga gatcacatct cctggcgtcg cgagcattcg ccggaactgc
    10021 cgcattgaca agttatgtca gcaagaaatg ccaatgaggg tgggcgctcc gttataccta
    10081 aacctggaaa ggcagtgccg caaagacagt cacgcttcgc gtgggctcat gcccggcgat
    10141 tccttcgcag actaatcttg agtttcctat cctggcgcgc taaattgcgg ctcgcggatc
    10201 attgtgattg ccctcgtgga cgcggtacgt cacccggttt gtaacgtatt agtaaggatt
    10261 gctgatgccg aatcggcggg ccggcgaaga gattgatttg agcgatcggc cgtctggacg
    10321 tagcgaagct tcgctggctg gctggatggc cctgggactg gcggggctgc tcggcattct
    10381 gagccttctg aaaaccatgc ctgtcgatct gcgctggagc gcggtgctgg cgagcgcgtg
    10441 cctgaacgtg ttcgttgtca cgatatggcg gccgccaaga ccgcgcacgc cacgcgatcc
    10501 ggcacaggga tcggcttgga agcagggcgt aagtttcatc agacggctgg tttggcaaga
    10561 tgccgaggcg ccgcccttga gtctcgcagt cagcggtttc cactggcagc cgtggggcgt
    10621 ggccattggc aactgtcttt ccgtggcgat cgccgcgacg gccttgcagc cgctgccgcc
    10681 cgcgcttatt cacgtgatgg tgacatcgtc ccttgtgatc gttagcggcg ctgctgcgct
    10741 gacgctggcc tttctcacgc tcgtcgccga acgcttctat gcacaggatg cggagcaatc
    10801 ccatgcttcg ctgtcggctt cgattgccgg catgctgcgc gtcctggtgg tgtgcgcgct
    10861 cgctgcggcg ctatcggcgg cgtggctcgt ctaccagcac acggcactcg attggctggt
    10921 gcatgtcgcg gcggtgttca cggctacgat tgccgtggaa atgatgatcc gcgccacgct
    10981 tctccggttc ttcgcaccgc gccccggtag aaacccggtg cgtgtcccgt cgagcatcat
    11041 tgcggggatg ctgcgttctc gcggctcatc gctctcgtcg atcggcgccg agttgcatga
    11101 gcgctacggt atcgatctgc gtcagaactg ggtgttgcgc agttttgtgc ggctgttgcc
    11161 ggtcaccgtg gtggcgatgg ctgcgtgtgc gtggttgttg acgtccgtca ccgtgttggg
    11221 cccgtcccag cgcgcggtgt acgaacgatt tggtgcgccg gtagcggttt ggccgccggg
    11281 cgtccacttc ggtatgccgt ggcctttcgg caaggctcgc ctcgtcgata acggcgcggt
    11341 gcatcagatc attgtgtcag gcggcgccga cgacagcagc gtcgccgcgc cagccactcc
    11401 cgccgacggc ccgacgtccg aacagatgaa tcgtctctgg gacgtccagc atccgtggga
    11461 cacgagccag gtgatcgcgg gtgccaccgg tgagcagcag agctttcaga tcgtcaacgc
    11521 cgacgtacgg ctcgactacc gcgagggctt gagcgacgcg gcggcgcgcg cggcgctcta
    11581 ccggtcggtc gatccggaga gcacagtgcg ctcgatcgcc aatcgcgagg tggtgcatta
    11641 cctcgcgtcg catacgctgc aggccttgct cgagacgagc cagaccgcca tggccgagag
    11701 tgtgcgcggt gccgttcaac ggcaactcga tcagctttct agtggcatcg aagtcgtggc
    11761 ggtggtgatc gagagcgtgc atccaccgtc gggggcggcc gcggcttatc acgacgtgca
    11821 ggccgcgcag attcgtgcgc aggcgagtgt tgccgaggcg cacggctttg cggcgggatt
    11881 gctcggcgat gcgcagcaac aggcccaggc aagcgtcacc caggcgagcg cccgggcggc
    11941 cgacacgatg gcggcggcgc gcgtgcagca gatcaacttc gaagccgaca cggtcgcata
    12001 ccggctcggc ggcccggctt ttcccttcga gtactacctg agcaagttgc agaaaggatt
    12061 gcagaacgca cgcatcacgg tgatcgacga ccggctgacc gccgaccgcc gcgcaaccat
    12121 cgacttgcgc tcgttcaccg cgggcgatct cacgggcatt cgtcatggca attgacgtcc
    12181 gggccgcatc gtacacgcgt cgtacagaga cgcacgccct atgcgggcaa gctgttttta
    12241 aggtgacgat atgagtttgt tccattcaca tgcagacggt cacgatcacg ggcatcgcca
    12301 tcatcatcac gatggcggcg ctgcgcaggg cgcgcgcggc gggccgttct atggccgggt
    12361 tctgcttgcc ttgctttgtg tggccgtggc gatcgcggtg gcgagtttca tctccgtgcg
    12421 cgcgggcgaa gcctcggtga tcacccgctt tggccaaccg gtgcgcgtgc tgctcgaacc
    12481 gggcctcgcg tggcgtctgc cggcgccgat cgaagcggtg gtgccggtcg acctgcgctt
    12541 gcatacgacg tcgagcggct tgcaggacgt gggcacgcgt gacggcttgc gaatcatcgt
    12601 gcaggcttat gttgcgtggc gcgtcgcggc tgatccgaac gacgtgcgcc gtttcgttcg
    12661 ggccgtgggc aaccagcctg acgaagccgc gcagcaaatc cgctccttac ttggctcgtc
    12721 gctgcaaact gcgtcggcgg ggttcgacct tgcgtcgctg gtcaatacgg atccgtccaa
    12781 ggtgcgtatc gccgcgtttg aagacgcgtt gcgtagccag atcgacgcgc agttgtactc
    12841 ggcttacggc gtgcatgtcg tgcaggtggg cctggagcgc ttgacgctgc ctgccgtgac
    12901 gcttgcggcc acggtcagcc ggatgagcgc cgagcgcgag acggtagccg cacagcgcac
    12961 ggccgagggc aaccgcgagg ctgcacagat ccgctcggat gcggaccgcg atgcccggct
    13021 tgccgtggcc gatgcgaatg tgaaagcggc gcagatcgag gcgcagtccc gcaaggacgc
    13081 cgccgaaatc tatggcgcca cctacgcggg caatccgcgg ctctacacga tgttgcgctc
    13141 gctcgacacc ctcgactcgg tggtcagcac caatacgaat ctgattctgc gcaccgacgc
    13201 cgcgccgttc caggcgctgg tacagggccc caccgcactg agccccgagt cgttgccgca
    13261 gcctggcgcc ggctcggcgt ccgggtcgaa aaaaatcagg tccgcgcgat gagcacggac
    13321 accggcgtgc cgccgctcgg ccccggcgct caggcggtcc gcctgtcgtt ctggttcgtc
    13381 gccgcgctgg tcgtgctcgc cgcttgcgca tgggccgggt cgaacatccg gcggatcccg
    13441 ccggatagcc gggcggtcgt gttgcgcttc ggttcgttcg tgcgcacaca ggacgcaggc
    13501 ctgttgattg cctggccacg cccgttcgag tcgaccttgc tggtgcccgg cagtgcgcgg
    13561 gttctggagc aacgcatcca gtcgctcgaa cgtgatccgc gtgcgcgcgc cgcggatatg
    13621 aatttgccgg aagctgcacc atcggctcaa gggcccgccg gaaccggcgc cgtgatcggg
    13681 cctgaggatg gtcctcccgg cccaccgccc gcgccggaca acgcggccga tcctgacgcg
    13741 gccccgcccg ttgcacagct gcccgatgcg ctggccggct ccggttatgt gctcaccggc
    13801 gacaacggcg tgctgcagtt gcgggccacg ttgtactacc gggtcgtcga tccctatgcg
    13861 tatgtcctgc aggaagaccg gctcgctgcg gcgctcgagc ggatcgtgtc cgcctcggcg
    13921 gtcagcgtcg tggcccagcg tgatctggat gcgattctcg tcgcgcgtcc cgaattgttg
    13981 tcggccgatc agcaggtcgc gataaagcgt gagcgtttgc ggggcgatct cgcgcaggaa
    14041 gtcgaacgcc acttgcgcga gctcgaagca caacatgcgg gactcggcgt ggaagtcgcg
    14101 cgtgtcgacg tcgaggtggc gtttccggcc gccgccgtga gcgcgttcaa ttcagtgctg
    14161 acctcgttgc aggtggccga gcgcagcatg gccgaggcgc gcacgtccgc tgaaaagaca
    14221 cgccaggacg cgcaacagga tgccgaccgg atcgtacaga acgcgcaggc gagcgccgtt
    14281 gagcgtgtcg ccaccgcgca ggctaaaacc atgaccattc agcaactgga agtgccgctt
    14341 cgcaccaaca gcgatcccgg gttggtggtg cgcctgtatc gcgatcgcgt ccaatcgatt
    14401 ctctcgaaag cgggcagcgt gacgaccgtc gggccgaacg acacctcgaa tctgatcctg
    14461 ccgggaaata cgcgatgagc acccgagtcg agcacacgac gcacccgggc gggcatgtcc
    14521 acacccacac cccgcacgcc gacgaagcga acggtgtcgc tcaggggcgc gcgattgttt
    14581 tgtcggcctc cgagcaacgc ggcgtcacgc ggcaagtcgc gcttgcattg ctggccggcg
    14641 gcctgctgat tctgtcgacg gcatggcaca cctttgcgcc agcggatgaa gcgcttgcgc
    14701 agattctggc cggcctcgct tcgctggttg tcgccggtcc agtgtttgcc ggcgcgtggc
    14761 acagcctgaa gtcccccagt ctgcacggtg tgactgaccg tttgatcgca ctcgcgatgc
    14821 tggcggcgtg ggcggtgggc gacatggcaa cggccgcatt gttgccgatc gtcatgacgt
    14881 tcggacacgc actcgaagag cgcagcgtgc tcggatcgca ggaggcgatc cgggcgctcg
    14941 gcaggctgac ctcgggcaag gtgcggcgcg tgaaggccga tggttcgatc gaagaagtgc
    15001 agtatgacgc ggtgcgggcg ggcgaccaca tcgaagtcgt cgccggtgcg cggattcctg
    15061 ccgacggcgt ggttcagcat ggcgactcca gtgtcgacaa tgggccgatt accggcgagt
    15121 cggtgccgct cgatgtcgag ccgggctcga ccgtattcgg cggcgcgatg aatctcgacg
    15181 gtccgttgcg tatcgaagtc acccatgcgg gcaagcagtc gacgctcggc aagatcatcg
    15241 agttgatgca gcgtgccgag caggcgaagc cgccgattac acaggtactc gaacgctaca
    15301 tgggcggcta tttgtgcctc gtcctgctga ttgcggcgat cgtgtggttc gtctcggcga
    15361 atgcatcggc gatgctggcc gtgattgtcg cggcgtgtcc atgcgcgctg gtgctcgccg
    15421 cgccgtcgac ggccattgcc ggcattgcgg tcggcgcgcg tcacggcatc ctgttcagaa
    15481 gcgccgcgtt tctcgacaag tttgcgcaga tcgattcgct ggtgatcgac aagaccggca
    15541 cgttgaccta cggcgaattg cgtgtcgaag acgtgatgct ggaacccggc gccgacgcgg
    15601 cccaggttct cgaactcgcg gcgcgccttg gcggcagcag tgcgcatccg gtgagccggg
    15661 cgctgctgcg ttacgcgaca gatgcagcac cggctggatt cgaacgggcg ccgttcgaac
    15721 acacccgcga actacgcggg ttgggcgtcc ttgcacagac gccggaaggt gtcgcggtgc
    15781 tgggccgcta cggcttgctc gcggactacg tacaggcttt gccggaaccg cgccgcgaga
    15841 tcgaaggacc ttgcgtcggt cttgccttga acggccggtt gctcgcgtgg ttcgggtttg
    15901 ccgacgcact gcgtacggaa gcaaaggatg cgctggcgga gttgcgggag ctcgggctga
    15961 cacgccagac actgttgacc ggagaccgcg agccggtggc gcgcaaactg gcagcggaga
    16021 ccggcattgc cgtggtcgtt gcgcaggcac tgccgcacga caagctcgat tacgtcgtgc
    16081 gggaaatggc ggctggctat cacccgctgg tcgtgggcga tggcctgaac gatgtgcttg
    16141 ccatcaaggc cggtcccacc agtatcgcga tgggtgagcg aggcatcgac attgcggtgg
    16201 cgtcggccga cgtggtgctg ctcggagacg acttgcgtcg gatcccgaca tgtgtccgcc
    16261 ttgggcggcg ctgcctgcgc actgcggcga tcaatgccgc gatcggattg gggtggactg
    16321 tcgcggtgat cgcgcttgcc gcgaccggcg tgctcagcgc cgtgtggatt gcgcttttgc
    16381 acaatctggg cacgttcatt gtgatcgcga acgcggggcg gttgctgcga atggacgaag
    16441 gtcttccact gcccaccccc attcgggaag gcaacacatg aggctccccc gacggtgctt
    16501 gcatgccatc ggttggcgcc ggaggaccac gccgcgccga aacagacccc gatacatcgg
    16561 gcaataagtc tcgtaaagcg atagaggagg gtgcgggaaa agaggcggcg cattgacaac
    16621 cctccacctg aactcagcgc aaacttctaa cctcgctcgg cgtgacactt ccggcaaatc
    16681 gattgccgaa atgggttctc gtgtagttaa caacatccgc gacctgatcg tcactcatcg
    16741 cgtcgccaaa cgatggcatc gcctttctgc cgtgcagtac gttaacgatg acgtagggtg
    16801 ccgcttcaac tgccgggttg tcagcgagcg ccgggtagtg acccgcgccc tgtgcgcctt
    16861 ttccatctgg catatggcat cccgcgcagg tcgcgttgta aagcgctccg ccgctttttt
    16921 cggtaaagcg gtagcccggc gaaatcgtga aggttcgtgg tgcatcctga gccgaccccc
    16981 tcgcggcccc catcagcaac cccaccagca tcacgccgag gatcagcatc gccctatcca
    17041 ttgacgattc tccgatgcaa ccgggcaatc gcatccagcg acgacagcac ggcgccttcc
    17101 tgccatgccg ggatatggga cgcgtgctct ccggcaagga cgatccgtcc gtcgatctga
    17161 cagagattgg cgtaatgcgc tgttctcaac gcatccgtcc atgcggcgaa gcagccattc
    17221 gtgaacggtg atcggctcca gctcatcgcg aagccgttct ggtactcgcg tgcgtactgc
    17281 ggatgaatct ggctgccctg gctcaatgcc accgcgatcc gttcggtagg gggcagcgca
    17341 cccatctcgt agctggcggg cccccacatg taggcgccga gcagcaccgc aggaccggtc
    17401 gatccgtaac ctgttgccgg atagccgatc atcgagatcg gcaggtcagt atgcgtgatg
    17461 ccgccgtaga tctgctcgtc ttcttcccag aatcgccggc cgaattgcaa gccgattttc
    17521 accgatgtct cgtagggcac cgcatcgatc gcggcgcgca tggccgggcc gaccgcaatg
    17581 tcgatctgac tcagaatcga gagcggaatg gtgcacacca gccaatccgc gtgcgcgttc
    17641 cgttcgctcc gggtggtcgt atcggtatag cgcacggtga cgccttgttc gtcctgcaag
    17701 atggccgtga ctctggcgtt gaacactacg gcgctcgcga cctgcgaata aagcgcatgg
    17761 gcaatcctgt ccatgccgcc aacgggttga aaaatgctgc tctggaagtc ctggtcgctg
    17821 ccagacgaaa gccattgcca cagattcgat gcaagcaagg cgtcgcgtgg gagcagattg
    17881 gacggcacgg ccctgggcat caacccaccg ccaggcgcgg tcgcgaatcc gcggcgctca
    17941 ctgctgcgcc gtcccgcctg ataggcgcca tccggatcg
//
LOCUS       DATASH010000338         2562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5587453,
            whole genome shotgun sequence.
ACCESSION   DATASH010000338 DATASH010000000
VERSION     DATASH010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5587453"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1093
                     /locus_tag="VHV99_09495"
     CDS             <1..1093
                     /locus_tag="VHV99_09495"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004266738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEX3635706.1"
                     /translation="AADRDAVLTALREHGLSACSHVIGKANERDVIEIYRDAKKVYEA
                     PRAELHRTWSEVSWRIARLRDNPACADAEHDTLLDAADPGITPVLSFDPADDVAAPFI
                     GKGARPRVAILREQGVNSHLETAYAFDRAGFAAHDVHMSDLLAGRANLADFAGAVACG
                     GFSYGDTLGAGEGWAKTIRFNAQLADMFAAFFGREDTFALGICNGCQMMSSLASMIPG
                     AEAWPKFTRNKSEKFEARFSLVEVQASPSIFFAGMEGSRIPVAVAHGEGYADFSQQGD
                     KSKVAVAMRYVDHRGQATEQYPFNPNGSPEGITSVTTADGRFSVLMPHTERVHRAVQM
                     SWHPQGWGEIGTDASPWLRVFQNARRWLG"
     gene            complement(1232..2355)
                     /locus_tag="VHV99_09500"
     CDS             complement(join(1232..2094,2094..2355))
                     /locus_tag="VHV99_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611549.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEX3635707.1"
                     /translation="MKRKQYSVEQIVAALKQADLGMPVADLIRQLGISEQTYYRWKKQ
                     YAGLESNQVRELKQLQDENARLKKLVAELSLDKAILQDIATKKLAAPALKRQAVDYVV
                     GHYGLNTQRACRLVRQARSTHYYRSVKDPQTALRQRMREIAQTRVRYGYRRVHVLLKR
                     EGWRVSRNRVYRLYAEEQLQLRSKLPKRRKMVVSRRERCVPARPNEVWSLDFVADQLA
                     DGTRLRALTVVDVFSREALAIEVGKRLRAEHVVSVLNRLVAQRRAPKFLFADNGSEFS
                     GRLLDMWAYHYKVRIDFSRPGKPTDNSFIETFNGSFRDECLNLHWFVSLEEARREIEA
                     WRRDYNESRPHMALNDLTPGEFARQYSLRPVAEDINSVDD"
BASE COUNT          455 a          819 c          792 g          496 t
ORIGIN      
        1 cgcggccgat cgcgacgcgg tgctcaccgc gctgcgcgag catggcctgt cggcgtgttc
       61 gcacgtgatc ggcaaggcca acgagcgcga tgtgatcgag atctatcgcg acgcgaagaa
      121 ggtctacgag gcgccgcgcg cggagctgca tcgtacgtgg agcgaagtga gctggcgcat
      181 cgcgcgtctg cgtgacaatc cggcttgcgc cgacgccgaa cacgacacct tgctcgacgc
      241 cgcggaccct ggcatcacgc cggtgctgag cttcgatccg gctgacgatg tcgccgcgcc
      301 gttcatcggc aagggcgcgc gtccgcgcgt cgccatcctg cgcgagcagg gcgtgaactc
      361 gcacctcgaa acggcctatg cattcgatcg cgccggcttc gctgcgcacg acgtgcacat
      421 gagcgatctg ctcgcgggtc gcgcaaacct tgccgatttc gcgggcgcgg tcgcgtgcgg
      481 cggcttctcg tacggcgata cgctgggcgc cggcgaaggc tgggcgaaga cgatccgctt
      541 caatgcgcaa ctcgccgaca tgttcgccgc gttcttcggt cgtgaagaca cgttcgcgct
      601 cggcatctgc aatggctgcc agatgatgag tagcctcgcg tcgatgatcc ccggcgccga
      661 agcgtggccg aagttcacgc gcaacaagtc ggagaaattc gaagcgcgct tctcgctggt
      721 cgaagtgcag gcatcgccgt cgatcttctt cgccggcatg gaaggctcgc gcattccggt
      781 cgcggtcgcg cacggcgaag gctatgcgga cttctcgcag cagggtgaca agtcgaaggt
      841 cgcggtcgcg atgcgctatg tcgatcaccg tggtcaggcg accgagcagt atccgttcaa
      901 cccgaacggc tcgcccgagg gcatcacgtc ggtcacgacg gccgacggcc gcttctcggt
      961 gctgatgccg cacacggagc gcgtgcatcg cgcggtgcag atgagctggc atccgcaggg
     1021 ctggggcgag atcggcacgg acgcgagccc gtggctgcgc gtgttccaga acgcgcgccg
     1081 ctggctgggt tgatgagtca aggggctgtc gccgcaaacc tcgcagcaac aaaaaagccg
     1141 cccacatgaa ctgaccccgc aaagttggac aatttaaagc taggtcccag agggctgtga
     1201 accgcttggg ttttctggac agtgtcgagg gttagtcgtc gacgctattg atgtcttcgg
     1261 ccaccggccg gaggctgtat tgccgggcga attcgcccgg cgtcaaatcg ttgagtgcca
     1321 tgtgaggccg gctctcattg tagtcccggc gccacgcctc gatttcgcgt ctggcttcct
     1381 ccagtgaaac gaaccagtgc aggttcaggc attcgtccct gaacgagccg ttgaacgttt
     1441 cgataaagct gttatccgtt ggcttgcccg ggcgactgaa gtcgatgcga accttgtagt
     1501 ggtatgccca catgtccagc agtcgtcccg agaattcgct gccgttgtcg gcgaacagaa
     1561 acttcggcgc tcggcgttga gccaccagcc ggttcagcac cgacacgacg tgctccgccc
     1621 gcaagcgctt tccgacttcg atggcaagcg cttcacgact gaagacgtcc acgaccgtca
     1681 gggcccgcag gcgcgtgccg tcggccaact ggtcagccac gaagtccagg ctccagacct
     1741 cattgggacg agccggtacg caacgctctc ggcgcgacac gaccatcttg cgccgcttgg
     1801 gcagcttcga gcgcagttgc agctgttctt cggcgtacaa ccgatagact cggttgcggc
     1861 tcacgcgcca gccttcgcgt ttgagcagca catgcacgcg ccggtagcca tatcgcacac
     1921 gagtctgggc gatctcgcgc atgcgctggc gcagcgccgt ctggggatct ttgacgctgc
     1981 ggtagtagtg cgtgctgcgc gcctgcctga ccaatcggca tgcccgctgc gtgttgaggc
     2041 cgtagtgacc gacgacgtag tccacggctt gcctcttcag cgcgggcgcg gccacttttt
     2101 tgtggcgatg tcctgcagga tcgccttgtc cagactgagt tccgcgacca gcttcttgag
     2161 ccgcgcattc tcgtcttgta actgcttcag ttcgcggacc tggttcgact ccaggcccgc
     2221 gtactgcttc ttccaccggt aatacgtctg ctcggaaatc cccaactgcc ggatcagatc
     2281 ggccaccggc atgcccagat cggcttgctt gagcgccgcc acgatctgct ccaccgagta
     2341 ctgtttgcgt ttcatcgcca cctccctcaa agggaaacgc gcgtcgaaac actaacattc
     2401 atttctgtcc aggattttta aagcagatca gcaggcacga cttaactcat aaccgagcat
     2461 gaagagcgcc gcctccaatc tacgaagtcc ctcgggcccg gcgcaccaat ttgcacgagc
     2521 tttatcacgc gctgcagaga gaacccattt cgcctgcaca tt
//
LOCUS       DATASH010000339         1660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2816518,
            whole genome shotgun sequence.
ACCESSION   DATASH010000339 DATASH010000000
VERSION     DATASH010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2816518"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VHV99_09505"
     CDS             complement(<1..315)
                     /locus_tag="VHV99_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635708.1"
                     /translation="MLTMGLYRQAMADQPLAAQSADQTVEVDVTARRQTALDAEAMSI
                     SETADAEPWTGKRSIIVGLAPVYGPVYDGAKKSKVSPFPYVDVQGLFGGRVFVSDFRG
                     LGV"
     gene            complement(535..1113)
                     /locus_tag="VHV99_09510"
     CDS             complement(535..1113)
                     /locus_tag="VHV99_09510"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3635709.1"
                     /translation="MIVKTQRQQPTPFDFIQQATRKHRWRHWCSAYAFKDGAGLIEIA
                     SRYSPARVIELGTALGFTACCLASSGKAARVDTIEGDSQHVALAQTNIAAAGLAERIT
                     VHLGRFEEVLRTLLGRYDMAFFDGGSPSVPIIEQLHALLDDDGVLICANLSRASAGQA
                     RQLKREFDNAHRWLHIGAIENGSTRVFRKQGK"
     gene            complement(1110..>1660)
                     /locus_tag="VHV99_09515"
     CDS             complement(1110..>1660)
                     /locus_tag="VHV99_09515"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3635710.1"
                     /translation="PVLECAERLHVPLYVHPSMPPQSVRNAYYDGLPGYASFALSIAG
                     WGWHAETAVHILRLALSGALDRHPRLKLLIGHMGEGLPAMMERVERVFAGYSKEHLGR
                     NAARAIVDQVWVSTSGFTGLPAFLATLMTFGIDRILFSVDYPFGNNRAAAEFLAKLPL
                     SPGDLLKVAHTNADGLLGLDAS"
BASE COUNT          347 a          501 c          483 g          329 t
ORIGIN      
        1 gacccccaag ccgcgaaagt ccgatacgaa aacgcggcca ccgaacagtc cctgaacgtc
       61 gacatacggg aatggcgaca ccttcgattt cttcgcgccg tcgtacaccg ggccatacac
      121 gggtgccagt cccacgatga tcgaccgctt gccagtccag ggctccgcat ccgcggtttc
      181 cgatatgctc atcgcctccg catcaagggc ggtctgtctc cgtgccgtta catccacctc
      241 aactgtttgg tcggcactct gcgccgcgag cggctgatcc gccatcgctt gcctgtacaa
      301 acccatcgtc agcatcagaa caccgacgat acgcgcagcc gtcgtcgggg cgcgtcgatc
      361 gggcgccgct gacgaccgac aatcttcggc gtccgctgcg ggcatgcacg cagcgatggc
      421 actaccggca agggccggcg aggaagactg ctgcgagttc gtcagatatt cagacatcat
      481 ggtctccggt aagcgcttgg cgctgccatt ggcacaacgc ggcgatgtag aaaattactt
      541 tccttgcttg cggaacacgc gggtgctgcc gttctctatc gcaccgatgt gcagccagcg
      601 atgcgcgttg tcgaattcgc gctttagctg tcgcgcctgg ccggcactgg cacgagacaa
      661 attggcgcat atcaagacgc cgtcatcatc cagcagggcg tggagctgct caatgattgg
      721 gacagatggc gagccgccgt caaaaaacgc catgtcatag cggccgagca gggttcgaag
      781 aacttcttca aaacggccca gatgcaccgt gatgcgttcc gcgaggcctg ccgctgctat
      841 gttggtttgc gccaatgcaa catgctggga gtcaccttct attgtatcga cccgtgctgc
      901 ttttcccgac gaggcgaggc agcaggcggt gaaaccgaga gccgtaccaa gctcgatgac
      961 gcgcgccggt gaatatcggc ttgcaatctc gatcagtccg gcaccatcct tgaaggcata
     1021 ggccgaacac cagtggcgcc agcggtgttt gcgcgtcgcc tgttggatga agtcgaaggg
     1081 cgtgggttgc tggcgctgcg tcttgactat cacgacgcgt cgagtcccag taggccatcc
     1141 gcgttcgtgt gagctacctt cagcaaatca ccgggcgata agggaagttt cgctagaaat
     1201 tcggctgccg cgcgattgtt gccaaacggg taatcaaccg aaaatagaat acggtcgatg
     1261 ccaaatgtca tgagtgtggc cagaaaggcc ggaaggcccg tgaatccgct ggtcgatacc
     1321 caaacctgat caacaatggc tcgcgcagcg tttcgcccta gatgctcttt gctgtatcca
     1381 gcaaacactc gttctaccct ttccatcatg gcgggcagac cctcacccat gtggccgata
     1441 agcagcttca gtcgcgggtg tcgatccagc gcgcccgaga gtgcaagtct taaaatatgc
     1501 accgcggttt ccgcgtgcca accccaacca gcgattgaaa gcgcgaagga tgcgtatccg
     1561 ggaagcccat cgtaataggc gttgcgaacg ctttgtggtg gcatgctggg atgcacgtac
     1621 agcggcacgt ggagtcgctc tgcacactcc agaacgggct
//
LOCUS       DATASH010000340         3022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4598869,
            whole genome shotgun sequence.
ACCESSION   DATASH010000340 DATASH010000000
VERSION     DATASH010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4598869"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VHV99_09520"
     CDS             <1..240
                     /locus_tag="VHV99_09520"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX3635711.1"
                     /translation="LLRPDIVKLCITVIRNADKGSHFMAAIRESAERLCKLGCLVLAE
                     GVEREHQHAALQGCGIELAQGWLYGKPEAVESVLR"
     gene            629..2212
                     /locus_tag="VHV99_09525"
     CDS             629..2212
                     /locus_tag="VHV99_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013696248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX3635712.1"
                     /translation="MNTNKKTLTIRARLIAGFGVVLIALVALTVIAISRVEMVRARLD
                     DIIDVNGVKERYAINFRGSVHDRSIAVRDVTLVSAAELPSVINHINVLASQYEESAGP
                     LARIYAERTDITAEERAIYARVNAAREQSLPLIQQLISLQQAGDVEGARKILLDDARP
                     ALVEWLAGINALIDYQEHLDQQEGAEARKISTDFRWLMSVLTLLACGVGIAVVYATVR
                     NITQSLGAEPSDVVELAESIRAGNLARGIELRPGDTSSVIAAMARMQQRLAEIVTQMR
                     DAAQGVAAATEQIALGNADLSTRTERQAAALEQASGALEEFDSSVAANAQSAGNADEL
                     AKHASATSAQGGTVVGQVVNTMRAISESSNRIEDIISVIDSIAFQTNLLSLNAAVEAA
                     RAGAGGRGFAVVASEVRMLAQRSAAAAKEIKELIGASSARISEGERQVDAAGTTISNI
                     VEASQRVTHIMEAMHQACRAQTQQIGEVRGVIGNLEQNTQQNVTLVQQSAAAAQTLKE
                     QAQGLLDSAQVFTLPFQTH"
     gene            2517..>3022
                     /locus_tag="VHV99_09530"
     CDS             2517..>3022
                     /locus_tag="VHV99_09530"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3635713.1"
                     /translation="MKILFAGVTPAFIAFLKPVLSSSGCDVIVASDGRDALDTFCTER
                     VDLILMDMDVRVMSGLEITGKIRVIEAMQELPWTPIVVLTAPDSLENLVTVIQAGADD
                     LVSQTAPEDVVKAKILAMVRTSTVRRQLETANGTLNNILNSVSEGVHVLDMHGVIIAE
                     NAASMIMFG"
BASE COUNT          648 a          856 c          944 g          574 t
ORIGIN      
        1 ctgttgcggc cggacatcgt caaactttgc atcacggtga tacgcaacgc cgacaagggc
       61 tcgcacttta tggccgcgat tcgtgagtca gccgagcggc tctgcaagct gggttgcctg
      121 gtgctcgccg aaggcgtgga acgagaacat cagcatgccg cattgcaggg ttgcggtatc
      181 gaactcgccc aaggctggct ttatggcaag ccggaggccg tcgagtccgt cctacgctga
      241 cggtctgatc gcatgggacc gcaaaagaca atctggaagg accgctaaga cctgccggac
      301 gccgaagaaa aagacgaccg ttgcacctcg aaagatgccg tttgcccggt gagagggcga
      361 gcggcggagg aagggcgccg gcatgaacga tgctgcgaag atccaggatt cctgttcggg
      421 tgaccacgca acggcaggca actgcggtac gcctgcggat atcgatagtc aaggtcaatt
      481 gatactttat tcgcaatata aataaaatat cgaaaagaga ttttgttgtt attattagac
      541 cccgaaacag aaacggcaag ccgcgcaggg ttgcttgccc agcaacggtc cacacaagtc
      601 aaggctacgt cagcacacgg ggaaccccat gaacacaaac aagaagacat tgacgattcg
      661 ggcgcggttg atcgcagggt tcggcgtggt tctcattgcg ctcgtcgcgc tgacggtcat
      721 cgcgatcagc cgggtggaga tggttcgggc gcgactcgac gacatcatcg acgtaaacgg
      781 cgtaaaggaa cgttacgcaa tcaactttcg cggaagtgtc cacgaccgct ccatcgcggt
      841 gcgtgacgtg acgcttgtat ccgcggcgga actgccttcg gtcatcaatc acattaatgt
      901 gctcgcgagc cagtacgagg aatcggctgg accgctcgcc aggatctatg ccgaacgtac
      961 cgacatcacc gcggaggaac gggcgatcta tgcacgtgtc aacgccgcgc gggagcaaag
     1021 cctgccgctg atccagcagt tgatcagctt gcagcaagcg ggcgacgttg agggcgcacg
     1081 caagattttg ctcgatgatg cgcgtcccgc gcttgtcgaa tggcttgctg ggatcaacgc
     1141 gctgattgac tatcaggaac acctcgatca gcaggaaggc gccgaggcgc gcaagatcag
     1201 cactgatttt cgctggttga tgagcgtgct gacgctgctc gcgtgcgggg ttggcatcgc
     1261 cgtcgtctac gcgaccgtgc gcaacatcac gcagtccctc ggggccgagc ccagcgacgt
     1321 ggtcgagctg gccgaatcga tccgcgcagg caaccttgcg cgcgggatcg aattgcggcc
     1381 cggcgatacg tcaagcgtga tcgcagcgat ggcgcgcatg cagcaaaggc tcgccgaaat
     1441 cgtcacgcaa atgcgcgacg ccgctcaggg cgtcgcggct gccacggagc aaatcgcgct
     1501 gggtaacgcc gacctgagta cgcgcaccga gcggcaggca gctgcgctcg aacaggcgag
     1561 cggcgcgctc gaggagttcg actccagcgt ggcggccaat gcgcaaagcg cgggcaacgc
     1621 cgacgaactg gcaaagcacg cttcggctac ctcagcacag ggtggcaccg tggttggtca
     1681 ggtcgtgaac acgatgcgcg cgatcagcga atcgtcgaac cgcatcgagg acatcatctc
     1741 cgtcatcgat tcgatcgcat tccagactaa tttgctttcg ttgaatgccg cagtcgaagc
     1801 ggcgcgggcc ggtgccggcg gtcgcggctt cgcggtcgtc gccagcgagg tgcgcatgct
     1861 cgcacagcgc agcgcagctg ccgcgaagga aatcaaggaa cttatcggcg cgagcagcgc
     1921 gcggattagc gagggggagc ggcaggtcga tgcggccggc acgacgatct ccaacatcgt
     1981 cgaagcatcg cagcgcgtca cgcacatcat ggaagcgatg caccaggctt gccgtgctca
     2041 gacgcagcag atcggcgaag tacgtggcgt gatcgggaac cttgagcaaa acacgcagca
     2101 aaacgtgacg ctggtgcagc agtcggccgc tgcggcgcag acgttgaagg agcaagcgca
     2161 gggtttgctg gactctgcgc aggtattcac gctcccgttc cagacgcatt gaacgagccg
     2221 gtcgttaata tctggcagag atgtactggg cgcggcttca tcgatgttgg agccgaccag
     2281 tggatcaaga aacgccttaa cggcttgtcg tctgatcgtg ggctagcact acacatccgc
     2341 ggaccatctg ttctcccgac cttttgccgt cggaggcgcc ggcggatgca cttgaagact
     2401 ggcttgggac ccgcgaggtg ccgcttgatt atgctccagg cttggcgcat catctgcttc
     2461 ggcagtatct ctgtccccga ttgtatctgg ctccgcgctt ctcagaggtc gcagtcgtga
     2521 agatcctgtt tgcaggtgta acccctgctt ttattgcctt cctgaagcct gtcctgtctt
     2581 catccggctg cgacgtgatc gtcgcctccg atggacgcga cgcactcgat acgttctgca
     2641 ctgaacgcgt cgatctgata ttgatggata tggatgtgcg cgtcatgtcg ggactcgaga
     2701 ttaccggcaa gatccgggtg atcgaggcga tgcaggaatt gccatggaca ccgatagttg
     2761 tactcaccgc tccagattca ttggaaaacc ttgtcaccgt tattcaggct ggagctgacg
     2821 atcttgtttc gcaaacagcg cctgaagatg tcgtgaaagc gaaaatcctc gccatggtca
     2881 ggacctcgac agtgaggcgt caactggaaa cggcgaacgg cacgctgaac aatatcctga
     2941 actcggtcag tgagggggtc catgtcctcg acatgcacgg cgtgatcatc gccgagaacg
     3001 ccgcatccat gatcatgttt gg
//
LOCUS       DATASH010000341         2682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5785498,
            whole genome shotgun sequence.
ACCESSION   DATASH010000341 DATASH010000000
VERSION     DATASH010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5785498"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VHV99_09535"
     CDS             complement(<1..257)
                     /locus_tag="VHV99_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006998986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635714.1"
                     /translation="MASRHAHAIARRCKKRCMIGTRQLPSPWHTCAPMSLGFCWVETL
                     LYGIIRFSREHPAMTKYVFVTGGVVSSLGKGIAAASLAAIL"
     gene            complement(530..1333)
                     /locus_tag="VHV99_09540"
     CDS             complement(530..1333)
                     /locus_tag="VHV99_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3635715.1"
                     /translation="MKNIIHFSHANGFPASTYRTIFAELADDYELRSIERIGHDARYP
                     VTQDWPHLVEQLLDDVGRSYEQPVWLVGHSLGGYLSLMAALKKPQWVRGVVMLDSPVI
                     AGWRSSMLRVSQWTGLDERLSPAAATRTRRTQWASRDEAWRHFHSKPAFARWDERMLS
                     DYIDFGIPQSSPDGARSLAFDRRTEYQIYKTLPHTLGPRLVRGAPVPVGFIAGTRSKE
                     VRQAGLDATRRTTGGHVEWIEGSHLYPMEKPLETARAVQRMLRQLERGA"
     gene            complement(1444..>2682)
                     /locus_tag="VHV99_09545"
     CDS             complement(1444..>2682)
                     /locus_tag="VHV99_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEX3635716.1"
                     /translation="AAHTLLDLLAAGLRMLSAPAWALWRLPQPDAWALAAAAVGVLWC
                     LAPRGWPLRWAAPLTWLPLLLPAPPGPPHGSFRLTALDVGQGTSVLVETAHHALLFDA
                     GPGPESTHAGERVVVPFLQAHGITALDTLIISHADSDHSGGAPAVLDAIEVRQMVAAL
                     APANPLWSNARQHGADTLPCAAGQRWQWDGVEFAMLWPDAGPLQGKPNAHCCVLRVST
                     LPAALNLPVTASPLVAQSMPLTVGRAVAESMPAGTVQTAPPRMTALLAADIEAPVERV
                     LLARDRDALRAQVLVVPHHGSKTSSTEPFLDSIDPLIALFQVGYRNRFHHPNAGVFER
                     YKARHIELARSDADGAVRVDIGTGLSPAPSPAPRPNIGPGGRPAISAGVDTQSGPSPN
                     AAALTLERYRDTQRRYWMDR"
BASE COUNT          461 a          909 c          879 g          433 t
ORIGIN      
        1 tcgaggatcg cggcgaggga agcggcggca atacccttgc cgagggaaga tactacgccg
       61 ccggtgacga aaacatattt ggtcatcgct ggatgctcgc gggaaaaacg gattataccg
      121 taaagcaggg tctcaaccca gcaaaatccg agcgacatcg gtgcgcaagt gtgccacggc
      181 gatggcaatt gccgtgtgcc gatcatgcac cgttttttgc agcgacgggc aatcgcatga
      241 gcgtggcgtg atgccatcag gcccgccgtg gtgcctcttg cgggcaggct gccgtagcgg
      301 tgaactcgcg cttttgcgcc ggtggtcacc cgcgtaagcg gaccgcgccg tcatcacgct
      361 cgccgtgccg cctcttatga gcaggccgcc gtagcggtga actcgcgctt tcgcgccggt
      421 ggtcacccgc ataagcggac cgcgcggtca ccacgcccgc cgtgccgcct cttatgagca
      481 ggccgccgca ccggcgacct cgcgcgtcgc gcagcgcagc ggcatggtct taagcccccc
      541 gctccagttg gcgcaacatc cgctgcaccg cgcgcgcggt ttcgagcggc ttttccatcg
      601 gatacagatg gctgccttcg atccattcca catgaccgcc cgtcgtgcgg cgggtggcgt
      661 ccagacccgc ctggcgcact tccttcgaac gcgtgccggc gatgaacccc accggcaccg
      721 gggcgccgcg cacgaggcgg ggccccagcg tatgcggcaa ggtcttatag atctgatact
      781 cggtgcgccg gtcgaacgcg agcgagcggg cgccgtcggg cgaactctgc gggatgccga
      841 agtcgatgta gtcggacagc atccgctcgt cccagcgcgc gaacgccggc ttcgaatgaa
      901 agtgccgcca cgcctcgtcg cggctcgccc attgcgtgcg acgagtgcgg gtggcggccg
      961 cgggcgaaag ccgctcgtcg agtccggtcc attgcgacac gcgcagcatg ctgctgcgcc
     1021 aaccggcaat cacaggcgaa tcgagcatca ccacgcctct cacccactgg ggctttttca
     1081 gcgcggccat cagcgacaga tagccgccga gcgaatggcc caccagccac acgggttgct
     1141 catacgaccg gccgacgtcg tcgagcaact gctcgaccag atgcggccag tcctgcgtga
     1201 ccggatagcg cgcgtcgtgg ccgatgcgct cgatggagcg cagctcgtaa tcgtcggcaa
     1261 gctcggcgaa gatcgtccgg taggtcgacg ctggaaaacc gttcgcatgc gagaagtgga
     1321 tgatgttttt caactgcggc tatctccgtt ctgttttggc tcgcgggctc ggacccgggc
     1381 atttcgtgcg ggctgcgagc ccggcgacgc gtttgttcta actgcgcggc cggtagtcat
     1441 ccatcagcga tccatccagt agcggcgctg cgtatcgcga tagcgctcga gcgtcagtgc
     1501 ggcagcgttc gggctcgggc cgctttgtgt gtcaactccc gcgctgattg ccggccttcc
     1561 cccgggcccg atgttgggac ggggagcagg gctcggggct gggctcaaac ccgtgccgat
     1621 gtcgacccgc accgcgccgt ccgcatcgct gcgcgcaagc tcgatgtgcc gtgccttgta
     1681 acgctcgaac acacctgcgt tcggatgatg aaaacggttg cgatagccta cctgaaatag
     1741 cgcgatgagc gggtcgatag agtcgaggaa cggctcggtc gacgaggtct tgctgccatg
     1801 atgcggcacg accagcacct gagcgcgcaa cgcgtcgcga tcgcgcgcga gcaggacgcg
     1861 ttcgaccggc gcttcgatgt ccgccgccag caaagctgtc atgcgtgggg gcgctgtttg
     1921 aacagttcca gccggcatgc tctctgcaac tgccctgccg accgtaagcg gcatgctttg
     1981 cgcgaccagc gggctcgccg tgacaggcaa attcaacgca gccggcaacg tactcacccg
     2041 caacacgcag caatgcgcat tcggcttgcc ttgcagcggg ccggcatccg gccagagcat
     2101 cgcgaactcg accccatccc attgccagcg ctgccccgcc gcacagggta gcgtgtccgc
     2161 gccgtgttgc cttgcgttgg accatagcgg attcgccggc gccaatgccg ccaccatttg
     2221 acgcacctcg atcgcgtcga gcaccgccgg cgcgccgccc gagtggtcgg aatcggcgtg
     2281 gctgatgatc agcgtatcga gcgccgtgat gccatgcgcc tgcaaaaaag gcacgacaac
     2341 ccgttcgccc gcatgggtcg actccggtcc cggtccggcg tcgaatagca acgcgtgatg
     2401 cgccgtttcg accagcaccg aggtgccttg accgacatcc agcgcagtca gacggaagct
     2461 gccatgcggc ggcccgggcg gcgccggcag cagcagcggc aaccaggtga gcggcgcggc
     2521 ccagcgcaac ggccagccgc gcggcgccag acaccagagc acgcccaccg cagccgcggc
     2581 cagcgcccat gcatccggtt gcggcagccg ccagagcgcc caggccggag cggatagcat
     2641 ccgcaagccc gcggccagca gatcgagcaa ggtgtgcgcg gc
//
LOCUS       DATASH010000342         9762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_641411,
            whole genome shotgun sequence.
ACCESSION   DATASH010000342 DATASH010000000
VERSION     DATASH010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 9762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9762
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641411"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..982)
                     /locus_tag="VHV99_09550"
     CDS             complement(<1..982)
                     /locus_tag="VHV99_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX3635717.1"
                     /translation="MSSFDLRLSTLRAAYRAGTLTPRQVIGELLAKAAALNPEFQLFI
                     HLLSEAEIAPYLEWLDTQDIESLPLYGIPFAIKDNIDLAGIPTTAACPAFAYTPGESA
                     TLVAQLIALGAVPLGKTNLDQFATGLNGTRSPYGKCRNSVHPDYPSGGSSAGSSLAVA
                     LGVASFALGTDTAGSGRVPAGLNNLIGLKATKGLLSTAGVVPACRTLDCVTYFTASAR
                     EASELLALTTQADPRDAYSRKNPQWNNARAFGKLARFRFGVPSRLEFAGCAESPGLFA
                     KARAMLEAAGGKAVEIDFAPFVEAANLLYEGPWVAERYNVVGELIEANEEA"
     gene            complement(1002..4601)
                     /gene="uca"
                     /locus_tag="VHV99_09555"
     CDS             complement(1002..4601)
                     /gene="uca"
                     /locus_tag="VHV99_09555"
                     /EC_number="6.3.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004847 - urea carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase"
                     /protein_id="HEX3635718.1"
                     /translation="MFEKILIANRGAIACRILRTLRELEVTGVAVYAEADRASLHVSE
                     APIAHCLGDGPASATYLDSQKILEIARRENVQAIHPGYGFLSENANFGEACEAAGIAF
                     IGPTPAQLRAFGLKHTARAIAAEQGVPMLEGTGLLDDLPAALDAAAAIGYPVMLKSTA
                     GGGGIGMRVCWNSEELSTHFDVVKRLGQNNFSDAGVFLEKYIERARHLEVQIFGDGRG
                     NAIALGVRDCSVQRRNQKVLEETPAPCLPDGMSAALCDAAVKLAKAVNYRSAGTVEFV
                     YDSAAQQFYFLEVNTRLQVEHGVTEQVWGVDLVRWMIELAAGTLAPLQELAAGLTPRG
                     HAIQARLYAEDPGRDFKPSPGLLTDVAFPRADGRALRIDTWIEAGCEVPPYFDPMLAK
                     LIAWSPTRDAARHALNDALQSTRLYGVETNRDYLRQILDDRPFASGEPWTRCLEGLRY
                     RASTIEVLSGGTQTTVQDHPGRLGYWAVGIPPSGPMDDRALRLGNRLLGNATDAAALE
                     ITMSGPTLRFNTDAVVVVTGAHLSVLLDDIEQPLNTTLFVAAGSTLALGAIRGAGARA
                     YLCVRGGFDVAVYLGSRSTFTLGQFGGHGGRALRAGDVLHLHPLQDDSTGAVLANVPE
                     LETVRTLRVIYGPHGAPEYFSARYIEQFFDTEWQIHFNSSRTGVRLVGPKPEWAREDG
                     GETGLHPSNIHDNPYAVGAVDFTGDMPVILGPDGPSLGGFVCPVTIIEADLWQIGQLK
                     AGDKVRFVPVQIDAAREAARQRLGELQTLEFDAQPVFTKAAPLVSPIVLDTGADRERL
                     VARVSGDTHLLLEIGPAELDLVLRFRGHALMQSLESMALPGVIDLTPGIRSLQIHYQP
                     ERLPLSQLLALVERAWQHVVLQKDLRAPSRIVHLPLSWDDPACRLAIDKYMTTVRRDA
                     PWCPSNLEFIRRINDLDSIDAVKQIVFDASYLVMGLGDVYLGAPVATPLDPRHRLVTT
                     KYNPARTWTAENSVGIGGAYLCVYGMEGPGGYQFVGRTLQMWNRYREVADFAGRPYLL
                     RFFDQIRFYEVAADELQRIRADFPLGRYPLKIEETELSLADYQTFLTRESVGIEQFRV
                     RQQAAFQAERERWREAGAAEESFDAPVAEDVELAPLKEGDVAVESEIAGSLWQVRVST
                     GDTVEAGDVLLVIESMKMEISVCAPSAGTVGEIYVVPGSPVRAGQRVAVIERL"
     gene            complement(4778..5419)
                     /locus_tag="VHV99_09560"
     CDS             complement(4778..5419)
                     /locus_tag="VHV99_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea amidolyase associated protein UAAP2"
                     /protein_id="HEX3635719.1"
                     /translation="MTTTLTVSDKRPEHAVFRETLAAGEPWLHELKAGQTLRILDLEG
                     NQAVDTLFYSLADPRERFDPQRTLRRQNSLYLTTGTVLYSNLGNPMLTIVADTCGRHD
                     TLGGACAQESNTVRYALEKRYMHSCRDNYLRACAHDGRLSKQDIGANINFFMNVPVTS
                     IGGLTFEDGISAPGKYVELRAEMDVIVLISNCPQLNNPCNAYNPTPAELLIWN"
     gene            complement(5433..6137)
                     /locus_tag="VHV99_09565"
     CDS             complement(5433..6137)
                     /locus_tag="VHV99_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea amidolyase associated protein UAAP1"
                     /protein_id="HEX3635720.1"
                     /translation="MDVLLEETVPGGGHASLIVKRGQSLRITDLRGGANVSVMLINAS
                     EKSERLNLPDTLKCQHTAKLTAGHCLYSDMGRVLAAIVADTCGWHDSIGGVSNAEEVQ
                     ERYGTGRYQELRNAFYRNGTDNLLVEMGKWGLGIQDLLMTLNLFSRVDVTEAGALNFV
                     PGNSKAGDYVDLYAPMNTLVVLTAIQHPLDPHPDYAPKPAKLALSHAQRDVAELCRTA
                     CEENVRGFINTDRLFL"
     gene            complement(6154..6945)
                     /locus_tag="VHV99_09570"
     CDS             complement(6154..6945)
                     /locus_tag="VHV99_09570"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3635721.1"
                     /translation="MIEVRNVWKEYGDQIVLERLNLRIEAGEFCSLVGASGCGKSTFL
                     RLLLGQERATRGEILLDGQPLPDEPDAHRGVVFQRYSVFDHLTVIRNVMLGLELPRAK
                     FTGRLFGARRRAARDEAAAMLEHVGLGAALDKYPQQLSGGMQQRLAIAQALMMKPRVL
                     LLDEPFGALDPGIRKDMHDLLLSLWRESKLTIFMVTHDLKEGFTLGSRVLVFDKVRVD
                     PQAPGAYGARITYNIPLDHSRDSASSIHMAQAVVEGSSRVEAVSA"
     gene            complement(6942..7757)
                     /locus_tag="VHV99_09575"
     CDS             complement(6942..7757)
                     /locus_tag="VHV99_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008346577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX3635722.1"
                     /translation="MRLINRHPSRSGGLMLLLLPFIVLMAVYFTGSSMRLKVNPDDKL
                     LPGATQMSEAIKEVAFTEDKRTGEYLLWADTVSSLRRLGIGLLVSAVIALAAAIATGS
                     LPLVSATFSPFITVISMIPPLAVLPILFIVFGLDELSKVVLIVVGITPMIIRDLHSRA
                     REIPAELWVKAQTLGANSWTLILRLVLPQLLPRLLVAMRLSLGAAWLFLIAAEAIAST
                     DGLGYRIFLVRRYLAMDVILPYVAWITLLAWLIDELLRRVTQWSFPWYGGGAR"
     gene            complement(7794..8864)
                     /locus_tag="VHV99_09580"
     CDS             complement(7794..8864)
                     /locus_tag="VHV99_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative urea ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX3635723.1"
                     /translation="MRKLVRFLGIAAVSLATLASTSAFAAGRTDFKVCWTIYAGWMPW
                     GQAKTQGIVSKWAKKYGINVDVVQLNDYVESINQYTAGKFDGCAMTNMDALTIPASGG
                     VDSTALIVSDYSNGNDGVLIKGKGKQIANLKGQQINLVQFSVSHYLLARALESAHMSE
                     RDLKVVNTSDADIAGAFATPAVQNAVTWNPMLADLKAQPNVTEVFDSSKIPGEIMDMM
                     VVNTKTLHDNPALGKALTGAWFEMVALMHANSADSTTALTAMAKASGTDLAGFKGQLS
                     TTALFYTPQAALDFVTSPDMPKIMTRVARFSFDHGLLGQDAKSADVVGMAFDKGVVIG
                     NKSNVKLRFDPTYVEMAAAGKL"
     regulatory      complement(8923..9022)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00442"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="guanidine-I (ykkC/yxkD leader) riboswitch;
                     Guanidine-I riboswitches control genes whose products are
                     involved in modification or pumping out guanidine as a
                     toxic compound from bacteria; This is class of guanidine
                     riboswitches is based on the ykkC/yxkD leader; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="guanidine"
                     /db_xref="RFAM:RF00442"
     gene            complement(9294..>9762)
                     /locus_tag="VHV99_09585"
     CDS             complement(9294..>9762)
                     /locus_tag="VHV99_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3635724.1"
                     /translation="EKDHGKDVSRTVARKLVVYHRRAGGQSQFSALLELEPKSDRIQK
                     AIDYANANLRNPLSVEELADVASLSPRQFSRAFTAETGQSPAKAVEHLRVEAARLMLE
                     QGRHSMDVIADEVGLSDRERMRRAFLRTLGQPPQAIRRFGRQTRLPAEQADRT"
BASE COUNT         1824 a         3093 c         3057 g         1788 t
ORIGIN      
        1 cggcctcctc attcgcttcg atcaactcgc ccactacgtt gtagcgctcc gcgacccacg
       61 ggccttcata gagcaggttc gccgcttcga cgaacggcgc gaagtcgatc tcgaccgcct
      121 tgccgcccgc ggcttcgagc attgcgcggg ccttcgcaaa caacccaggg ctttcagcac
      181 agcccgcgaa ttcgagtcgc gacggcacgc caaagcggaa tctggcgagc ttgccgaacg
      241 cacgcgcatt gttccattgc gggttcttgc gactgtatgc atcgcgagga tcggcctgcg
      301 tggtgagcgc cagcagttcg ctggcttcgc gcgcgctcgc cgtgaaatac gtcacgcaat
      361 cgagcgtgcg gcaggccggc acgacgccgg cggtggacag cagacccttg gtcgccttga
      421 gtccgatgag gttgttcagc cctgccggca cgcggccgga gcccgcggta tcggtgccga
      481 gcgcgaagct cgcgacgccc agcgcgacgg ccagcgacga ccccgcactc gagccgcctg
      541 acggatagtc gggatgcacg ctgttgcggc acttgccgta cggtgaacgc gtgccgttaa
      601 ggccggtggc gaactggtcg agattggtct tgccgagcgg cacggcgccc agcgcgatca
      661 actgcgcgac gagtgtcgcc gactcgcccg gcgtataggc gaacgcgggg caggcggccg
      721 ttgtcggaat accggccagg tcgatgttgt ccttgatcgc gaacggaatg ccatagagcg
      781 gtaatgattc gatatcctga gtgtcaagcc attccagata gggcgcgatt tccgcttcgc
      841 tcagcaggtg gatgaagagt tgaaactcgg gattcagcgc agcggctttc gccagcagtt
      901 cgccgatcac ttggcgcggc gtgagcgtcc cggcacggta agcggcacgc agcgtggaca
      961 ggcgcagatc gaatgaagac atgaatgttt tcccaggaaa gtcaaaggcg ctcgataaca
     1021 gcgacgcgtt gccccgcgcg caccggcgag ccgggcacca cgtaaatttc gccgacggtt
     1081 cccgcagacg gcgcgcacac ggagatttcc atcttcatcg actcgatgac gagcagcacg
     1141 tcgccggcct ctaccgtgtc gccggtactc acgcgcactt gccagaggct gcccgcgatc
     1201 tcgctttcca cggctacgtc gccctctttc agcggcgcga gttccacgtc ttcggcgacc
     1261 ggcgcgtcga aactctcttc tgccgcgccc gcttcgcgcc aacgctcgcg ttcggcctga
     1321 aacgcggctt gctgacgcac gcggaattgc tcgatgccga ccgattcgcg cgtgagaaac
     1381 gtttgatagt cggccagcga cagttcggtc tcttcgatct tcagcggata tcggccgagc
     1441 ggaaagtccg cgcggatgcg ctgcaattcg tcggccgcga cttcgtaaaa gcggatctgg
     1501 tcgaagaagc gcaacaggta cgggcgcccc gcgaaatccg cgacttcgcg gtaacgattc
     1561 cacatctgca gcgtgcggcc aacgaactga taaccgccag gcccctccat cccatacacg
     1621 cacagatacg cgccgccgat gccgaccgag ttctcggccg tccatgtgcg cgccgggttg
     1681 tatttggtcg tcaccagccg gtggcgcgga tcgagcgggg tcgcaaccgg cgcgccgaga
     1741 tacacgtccc caagccccat cacgagatag ctggcatcga agacgatctg tttgacggcg
     1801 tcgatcgaat cgaggtcgtt gatgcggcgg ataaactcga gattgcttgg gcaccagggc
     1861 gcgtccctgc gcaccgttgt catgtatttg tcgatcgcga gccggcacgc gggatcgtcc
     1921 cacgacagcg gcagatgcac gatccgtgac ggcgcgcgca aatccttctg caacacgaca
     1981 tgctgccagg cgcgctcgac gagcgcgagc agctgcgaca gaggcaggcg ctcaggctga
     2041 tagtggatct gcagcgagcg gatgcccggc gtcagatcga tcacgccagg cagcgccata
     2101 gattcaagcg actgcatcag cgcatgaccg cggaagcgca agacgagatc gagttccgcc
     2161 gggccgattt cgagtagcag atgcgtgtcg ccggaaacac gcgcgacgag gcgttcgcga
     2221 tccgcgcccg tatcgaggac gataggcgag accagcgggg cggccttcgt gaaaaccggc
     2281 tgtgcgtcga attcgagcgt ctgcaattca cccagacgtt ggcgcgctgc ctcacgtgct
     2341 gcatcgatct gcaccggcac gaagcgcacc ttgtcaccgg ctttcaactg gccgatctgc
     2401 caaagatccg cctcgatgat cgtgaccgga caaacaaagc cgccgagact cggaccatcg
     2461 ggcccgagaa tcacaggcat gtcgccggtg aaatcgacgg cgccgaccgc atacggattg
     2521 tcgtgaatat tcgacggatg cagcccggtc tcgccgccat cctcgcgcgc ccactctggc
     2581 ttcggtccga ccagacgcac gccggtgcgg ctggagttga aatgaatctg ccattcggtg
     2641 tcgaaaaact gctcgatata gcgcgcgctg aagtactccg gcgcgccgtg cgggccataa
     2701 atcacgcgca acgtccgcac ggtttccagc tcagggacat tcgcaagcac cgcgcccgtg
     2761 ctgtcgtcct gcagcgggtg cagatgcaat acatcgcccg cgcgcagcgc tcgcccgcca
     2821 tgtccgccga actggccgag cgtgaaagtg ctgcggctgc cgagatacac cgcgacatcg
     2881 aagccgccgc gcacacacaa ataggcacgc gcacccgccc cgcggatcgc accgagcgcg
     2941 agcgtcgaac cggcggcgac aaagagcgtc gtgttcaacg gctgttcgat gtcgtcgagc
     3001 agcacggaca gatgcgcgcc cgtcaccacg acgaccgcat ccgtattgaa acgcaaggtc
     3061 ggcccactca tggtgatttc gagcgcggcc gcgtctgttg cattgccgag cagccggtta
     3121 ccgagccgca gcgcgcggtc atccatcgga ccggaaggcg gaatgccgac tgcccagtag
     3181 ccgagacggc ccggatgatc ctgcacggtc gtctgtgtgc cgccgctgag tacttcgatc
     3241 gtgctcgccc gatacctgag cccttcgagg cagcgcgtcc agggttcgcc cgacgcaaac
     3301 ggccgatcgt cgaggatctg ccgcagatag tcgcgattgg tctccacgcc gtagaggcgc
     3361 gtgctttgca acgcgtcgtt gagcgcatgg cgtgccgcgt cgcgcgtcgg cgaccaagcg
     3421 atcagcttcg ccagcatcgg gtcgaagtag ggcggcactt cgcagccggc ttcgatccac
     3481 gtatcgatgc gcagcgcgcg gccgtcggcg cgcggaaacg cgacatcggt cagcaagccc
     3541 gggctcggct tgaaatcgcg gcctgggtct tcggcgtaga ggcgtgcctg gatcgcgtgc
     3601 ccgcgcggcg tcaagcccgc cgcgagttcc tgcaatggcg cgagcgtgcc cgcggccagt
     3661 tcgatcatcc agcgcaccag atcgacgccc caaacctgct cggtcacacc gtgctcgacc
     3721 tgcaaacgcg tattcacttc gaggaagtag aactgctgcg cggcgctgtc gtagacaaat
     3781 tcgacggtgc cggccgagcg gtaattcact gcttttgcga gcttcacggc ggcgtcgcac
     3841 aatgcggcgg acatgccgtc cggcaaacag ggtgcggggg tttcttcgag taccttctga
     3901 ttgcgccgct gcaccgagca atcgcgcacg ccgagggcaa tggcattgcc ccgcccgtcg
     3961 ccgaagatct gcacttcgag atgccgcgct cgttcaatgt acttttcgag gaacacgccg
     4021 gcatcactga agttgttctg gccaaggcgc ttgaccacgt cgaaatgcgt gctcagttcc
     4081 tcgctgttcc agcacacccg catgccgata ccgccgccgc ccgccgtgct tttcagcatc
     4141 accggatagc cgatcgccgc cgctgcatcg agcgccgccg gcagatcgtc gagcaagccc
     4201 gtgccttcga gcatcggcac gccctgttcg gccgcgattg cacgagccgt gtgcttcaga
     4261 ccaaaggccc gcaattgcgc gggcgtcggt ccgatgaacg caatgccggc cgcttcgcac
     4321 gcttcgccga agtttgcatt ctccgacagg aatccgtagc ccggatggat tgcctgaacg
     4381 ttctcacggc gagcaatctc gagaatcttt tgcgaatcga ggtaggtggc cgacgccggg
     4441 ccgtcgccga gacagtgcgc aatgggcgcc tcgctcacat gaagacttgc gcgatccgct
     4501 tccgcataaa cggcgacacc ggttacctcg agttcgcgca atgttctaag gatgcggcac
     4561 gcaatcgcgc cgcgattggc gatcaggatt ttctcgaaca tgaaaggagg tcttgattca
     4621 tgggcgggcc gtcccgccac gtgggaattg catgccgcgg tcgtccgcgg cagagaccgg
     4681 cgttagtgat tcgcgtgggt caattgctcg cgcgtgcagg tacagcatcg tccggagcga
     4741 accagcatga cgaagtaaag ccagttgcgg atacgcttca gttccatatc agcagctccg
     4801 cgggtgtcgg gttataggca ttgcacggat tgttcaactg cgggcagttg gagatcagca
     4861 cgatcacatc catttccgcg cgcaattcca cgtacttgcc tggcgccgag atgccgtcct
     4921 cgaaggtcag accgccaatg gaggtcaccg gcacgttcat gaagaagttg atgttcgcgc
     4981 cgatatcctg cttcgacaaa cgtccgtcgt gtgcacaggc gcgcaggtaa ttatcgcggc
     5041 agctatgcat gtagcgcttt tccagcgcgt aacgcaccgt gttgctctcc tgtgcgcaag
     5101 ccccgccgag cgtatcgtga cgaccgcagg tatcggcgac gatcgtcagc atcggattgc
     5161 cgaggttcga atacagcacg gtacccgtcg tcaggtacag gctgttctgc cggcgcagcg
     5221 tacgttgcgg gtcgaaacgc tcgcgcggat cggcgagact gtagaacagc gtatcgacgg
     5281 cctgattgcc ttccagatcg agaatgcgta gcgtctgccc agctttcagc tcgtgaagcc
     5341 atggttcacc ggccgcgagt gtctcgcgaa agaccgcgtg ttcggggcgt ttgtcgctga
     5401 cagtaagcgt cgttgtcatg gtcgggctcc gctcaaagaa agagacgatc ggtgttgatg
     5461 aagccgcgca cgttttcctc gcacgcggtt cggcacagct cggcgacatc gcgctgcgcg
     5521 tggctcaacg caagcttggc cggcttgggc gcatagtccg gatgcggatc gagcggatgc
     5581 tggatcgcgg tgagtacgac gagcgtgttc atcggcgcat acagatcgac gtagtcgccg
     5641 gccttcgaat tgcccggcac gaaattcagt gcgcccgcct ccgtgacgtc gacccggcta
     5701 aagaggttga gcgtcatcag caggtcctga atgccgagcc cccacttacc catttcgacg
     5761 agcagattgt cggtgccatt gcgatagaac gcattgcgca gttcctggta gcggcccgta
     5821 ccgtagcgct cctgcacttc ttctgcgttc gatacgccgc cgatgctgtc gtgccagccg
     5881 caggtgtccg ccacgatcgc ggcaagcacg cggcccatat cggagtacag gcaatgaccc
     5941 gccgtgagtt tcgccgtgtg ctggcacttc agcgtatcgg gcaggttgag tcgttcgctc
     6001 ttctcgcttg cattgatcaa catgacgctg acgttggcgc caccgcgcag gtcggtaatg
     6061 cgcaacgact ggccgcgttt gacgatcagc gatgcatggc cgccgccggg gacggtttct
     6121 tcgagtagca catccatgtt gttgagattc ctgtcaggca gagaccgctt cgacacgcga
     6181 tgagccttcc accacggcct gggccatatg gatcgacgac gcggagtcgc ggctgtggtc
     6241 gagcggaatg ttgtaggtga tacgcgcacc gtaggcgccg ggcgcctgag ggtcgacgcg
     6301 gaccttgtcg aataccagca cgcggctgcc cagcgtgaag ccttccttga gatcatgcgt
     6361 gaccatgaag atggtgagct tcgactcgcg ccacaggctc agcagcaggt cgtgcatgtc
     6421 cttgcggatg ccgggatcga gcgcgccgaa tggttcgtcg agcagcaaga cgcgtggctt
     6481 catcatcagt gcctgagcga tcgcgagccg ctgttgcatg ccgcccgaca gttgctgcgg
     6541 atatttgtcc aacgccgcgc cgagacctac gtgttccagc attgccgcag cctcgtcacg
     6601 cgccgcacgc cgtcgcgcgc cgaacaatcg gcccgtgaac ttcgcgcgcg gcaattcgag
     6661 tcccagcatc acgttgcgaa tcaccgtgag atggtcgaac accgaatagc gttgaaacac
     6721 cacgccgcga tgcgcatcgg gctcatcggg caacggttgc ccgtccagca gaatctcgcc
     6781 gcgggtggcg cgctcctggc cgagcaacag ccgcaggaag gtcgacttgc cgcagcccga
     6841 tgcgccgacc agcgaacaga actcgcccgc ttcgatacgc aggttcaatc gttcgagcac
     6901 gatctgatcg ccgtattcct tccagacgtt acgaacttcg atcatcgggc gcctccgccg
     6961 taccagggaa acgaccattg cgtcacgcgg cgcagcagct cgtcgatgag ccacgcgagc
     7021 agggtgatcc acgccacgta aggcaggatc acgtccatcg cgaggtagcg gcgcaccagg
     7081 aagatgcggt agccgaggcc atccgtcgaa gcaatcgctt ccgctgcgat cagaaaaagc
     7141 cacgccgcac cgagcgacag acgcatcgcc accagtagac gcggcagcaa ttgcggcagc
     7201 acgagacgca gaatcaacgt ccaactgttc gcgccgagcg tctgcgcctt gacccacaac
     7261 tcggccggga tttctctggc gcgcgaatgc aggtcgcgga tgatcatcgg ggtaatgcca
     7321 accacgatca gcaccacctt cgacaattcg tcgagcccga acacgatgaa cagaatgggc
     7381 agcaccgcga gcggcgggat catcgagatc accgtgatga acggtgaaaa cgtcgcgctc
     7441 accaacggca acgaaccggt cgcgatggcc gccgccagcg cgatcacggc gctcacaagc
     7501 aggccgatgc cgagccgccg caagctcgac accgtgtcgg cccacagcag atactcgccc
     7561 gtgcgtttgt cctcggtgaa agcgacctcc ttgatggcct cgctcatctg cgtcgcgccc
     7621 ggcagcagct tgtcgtcggg attgacctta agccgcatcg acgacccggt gaaatacacc
     7681 gccatcagca cgatgaacgg cagcaacagc agcatcaggc cgccgcttcg gctgggatgc
     7741 cggttgatga gtcgcatgga tgcccgcctt gtcgaatgcg tgagatgttg ggctcagagc
     7801 tttccggccg ccgccatttc gacgtaggtc ggatcgaaac gcagcttgac gttgctcttg
     7861 ttgccgatca cgacgccttt gtcgaacgcc atgccgacca cgtccgcact tttggcatcc
     7921 tgtccgagca agccatgatc gaacgagaac ctggcaacgc gcgtcatgat cttcggcatg
     7981 tcgggactgg tgacgaaatc gagcgcggct tgcggcgtgt agaagagcgc ggtggtcgac
     8041 aactggccct tgaagccggc gagatcggtg cccgatgcct tggccatcgc agtgagcgcg
     8101 gtggtgctgt cggcgctgtt ggcgtgcatc agcgcgacca tctcgaacca ggcgcccgtc
     8161 agcgccttgc cgagtgcggg gttgtcatgc agggtcttgg tgttgaccac catcatgtcc
     8221 atgatttcgc cggggatctt gctcgagtcg aagacttcgg tgacgttcgg ctgcgccttc
     8281 agatcagcga gcatcgggtt ccacgtcacc gcgttctgga ccgcgggcgt cgcgaacgcg
     8341 ccggcgatgt cggcatcgga cgtgttcacc actttgaggt cgcgctcgct catatgcgcg
     8401 ctctcgaggg cgcgggcgag caggtagtgc gaaacggaga actggaccag attgatctgc
     8461 tggcccttca ggttggcaat ctgcttacct ttgcctttga tgagcacgcc gtcgttgccg
     8521 ttcgaatagt cgctgacgat cagcgcagtc gaatccacgc cgcccgaggc cggaatggtc
     8581 agcgcatcca tattggtcat cgcgcagccg tcgaacttgc cggcggtgta ctgattgatg
     8641 gattcgacgt agtcgttcag ttgcacgaca tcgacgttga tgccgtattt tttggcccac
     8701 ttcgagacga tgccctgggt ctttgcctga ccccacggca tccagccggc gtagatggtc
     8761 cagcagacct tgaagtcggt acggcctgcg gcaaatgcgg aagttgaggc gagggtggcg
     8821 agggagacag cggcgatacc gagaaagcga acgagcttgc gcatgagatt gacctcggaa
     8881 gggacggtta gcgacggcag ggagcagcgc aaacatcgcg ttctctcccg ggcttttatc
     8941 ccgccgtgta acctcgcctg aggtcgacag ctctcggacc agcacctgcg tgaagcaggc
     9001 cggaacccta gccgtccatt tccagattgt gtgcaaaccg gatgatgcgc cggctccttg
     9061 ccatcgttgt agagcgagaa gcgtgccaaa cggaaatcgc cgcatttcgc ggcgtcgcag
     9121 tgggtgtctg ccctgttcca gtgatggcgc gcacccgcgc atgcggaacg ggcaccctat
     9181 ttgtgcattg attgcccggc gttgatgact atctggaggt ccggcccaaa gtttcctcgc
     9241 ggtctgatcg agcgaaaggc accaggatgc cttctccatc aggcatccct gaatcaggta
     9301 cggtcggcct gctccgccgg caggcgagtt tgcctgccga agcgccgaat ggcctgcggc
     9361 ggctggccga gtgtgcgcaa gaacgcgcgc ctcatgcgct cccggtcaga caggccgacc
     9421 tcatcggcga tcacatccat cgaatggcgc ccctgctcga gcatcagccg cgcggcttcc
     9481 acgcgcaggt gttcgacggc cttggccggc gactggccgg tctccgcggt gaaggctcga
     9541 ctgaactggc gcgggctcaa gctcgcgacg tcggcgagct cctccacgga caacgggttg
     9601 cgcagattgg cgttcgcgta gtcgatggcc ttctggatcc tgtcggactt cggctcaagc
     9661 tcgagcaacg cggagaattg cgactggccg cccgcgcgcc gatgatagac gacgagtttg
     9721 cgcgcgaccg tgcgcgacac gtccttgcca tgatccttct cg
//
LOCUS       DATASH010000343         6488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3215312,
            whole genome shotgun sequence.
ACCESSION   DATASH010000343 DATASH010000000
VERSION     DATASH010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6488
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3215312"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1102)
                     /locus_tag="VHV99_09590"
     CDS             complement(<1..1102)
                     /locus_tag="VHV99_09590"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEX3635725.1"
                     /translation="MALSRTVSEHRTVPASGRLFLSLLERIQVGHLVLITPDAQQRVF
                     GDPHAAPGARLALRDWRACGAILRKGDIGFADAWRAFWVDTPDLAALLRVAIRNERAL
                     QRTVYGGWLAQLWYGLRHKLRSNTRSGSRRNIHAHYDIGNAFYAQWLDPTFTYSSAVF
                     DGNFHQSLEDAQHAKYQRIIDTLGLREGMRILEIGCGWGAFAMHAARRGIHVHGVTIS
                     PAQLEFARKRVSEAGLTERVHLELRDYRTLTGQYDGIVSIEMFEAVGEKFWPTYFRIL
                     RERLRPGARALVQTITIDDSHFAAYRATSDFIREFIFPGGMLPSPRRFIEAARRGKLA
                     ARTSLTFGRDYAETLRRWRSAFEAQLEPIRAQG"
     gene            complement(1115..1972)
                     /locus_tag="VHV99_09595"
     CDS             complement(1115..1972)
                     /locus_tag="VHV99_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014725773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HEX3635726.1"
                     /translation="MNPPRADVDPAAWLLTGNVMHERLRPKHHRFTYPVFYVRCDLDR
                     LASLDREWFGIDRWRLLSLRQRDHGPRDGSDLATWMRAQLSAAGIEEADGRIWLQAFP
                     RVLGYAFNPVSFWFCHDRDGHLRALLAEVRNTFGGRHSYLLSAAGNAPITADTKLKCR
                     KVLHVSPFCRVEGSYEFRVRETPNSASVSIDYRDADGLLIRTALGGRLKPLTRSAALA
                     ALIRQPLLTVGVVARIHWQALRLAIKRAPFYGKHPAPGRTADASTDASTDHAATGQAS
                     AAARPISSD"
     gene            complement(1969..3270)
                     /locus_tag="VHV99_09600"
     CDS             complement(1969..3270)
                     /locus_tag="VHV99_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3635727.1"
                     /translation="MTQPAPASPLRCGARVAVIGAGISGLASAYLLARNHQVTLFESA
                     GYAGGHTNTVDVALEGHTHPVDTGFLVFNDRTYPNLVALFAELGVESHPSDMTFSVSL
                     DEGRLEWAGTSLNTVFAQRRNMFSPTFIGMLRDILRFNSSAQRNLEIATQTRASMGEL
                     LTAGGYGGAFQRHYLLPMAAAIWSSATADILAFPATTFLRFCLNHALLQVNRRPRWKT
                     VVGGGREYVRRIVGTLDDVRIATAVRGVRRAADGVDVFTDTGTERFDALVLATHAPTT
                     LRLLEDADPDERRVLGAVRYQPNVAVLHTDTNLLPRRQRVWSAWNYLGSRGVDGTHPV
                     CVSYLVNQLQPLPFNTPLVVTLNPVTQPAPGTELRRFVYDHPLFDLAAIDAQHRLPSL
                     QGKRRTWFAGAWTGYGFHEDGLKSALRVAADFNALPVWATL"
     gene            complement(3434..4081)
                     /locus_tag="VHV99_09605"
     CDS             complement(3434..4081)
                     /locus_tag="VHV99_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635728.1"
                     /translation="MSTVALAGTAATGKALANATININCAQGSISVGADVNGNYRATL
                     HAVMPCMITATSSGTVLHSVAFAGGTYNVTPETDLLLAYVAAQLGTNETGLIAGFPAS
                     TLFQQTLANQNGLLTAQAAVVQNIQQTYGVSLSAPNFLTTSFSVGQPGVDSDLEALFA
                     RGAIDANGEPDAAAVALMTTMGAAHPITVSPGTGTGTGSGGTGGTGSPGGIGGMM"
     gene            complement(4315..5592)
                     /locus_tag="VHV99_09610"
     CDS             complement(4315..5592)
                     /locus_tag="VHV99_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006480095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3635729.1"
                     /translation="MKSHYRWMVAALLFFAGMLNYLDRAALSVVAPIIKRDLHIDDAQ
                     MGILFSSFFIGYCLFCLIGGWAADRYGPRKVFMLAAGFWSVFCGATAFAGNYATLMVA
                     RVAFGVAEGPMGTTTNKSISNWFPRREAGRAVGITNAGQPLGAAIAAPIVGLVALQFG
                     WRVSFVVIALLGFVWMVFWWRCFRDQPTDHPAVSQEEAQLIASDRQVVNASDTSADDG
                     VMRYLLSMPVLGVAMAFFAFNYVLYFFLSWLPSYFTDYQHLDIKHMSIVGILPWLGAT
                     LGFVLGGLISDGLYRRTGNVLFARKTVIIMGLGMAAVCVLLVSRATSLAAAVSLIAVA
                     SLFAFMTPQACWSLLQDIVPRGRIGATGGFVHLLANLAGILAPSITGFMIQYGAGYSS
                     AFVLASALAAAGMLALWFSVREENVERLRRAPV"
     gene            complement(6174..6263)
                     /locus_tag="VHV99_09615"
     tRNA            complement(6174..6263)
                     /locus_tag="VHV99_09615"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6227..6229),aa:Ser,seq:cga)
BASE COUNT         1327 a         1975 c         2046 g         1140 t
ORIGIN      
        1 agccttgcgc gcggatcggc tcgagctgag cttcgaacgc actgcgccag cggcgcagcg
       61 tctcggcgta gtcgcgcccg aatgtcaacg acgtgcgcgc cgcgagcttg ccgcgccgag
      121 ccgcttcgat gaaccgccgc ggactcggca acatgccgcc cggaaaaatg aactcgcgga
      181 tgaagtcact agtggcacga taggccgcga agtgcgagtc gtcgatcgtg atggtttgca
      241 cgagcgcgcg tgcgcccggc cgcaaacgtt cgcgcagaat gcggaagtac gtcggccaga
      301 atttttcgcc gacggcttcg aacatttcga tcgacacaat gccgtcgtac tggccggtca
      361 gcgtacggta gtcgcgcagt tcgaggtgca cgcgctcagt caagcccgct tcgctcacac
      421 gcttccgggc gaattcgagc tgcgccggcg agatcgttac gccgtgcacg tgaatgcccc
      481 ggcgtgccgc atgcatcgca aaagcgcccc atccacagcc gatttccaga atgcgcatgc
      541 cttcgcgcaa accgagcgta tcgatgatgc gttgatactt cgcatgctgc gcgtcttcga
      601 gcgattgatg gaaattgccg tcgaacaccg cgcttgaata cgtgaacgtc gggtccagcc
      661 actgcgcgta gaacgcgttg ccgatgtcgt agtgcgcgtg aatattgcgc cggctgcccg
      721 agcgtgtatt ggacctcagt ttatgacgca gcccgtacca tagttgcgcg agccagccgc
      781 cgtagacggt gcgttgcagc gcgcgttcgt tgcggatggc aacgcgcagc aaggcggcca
      841 gatccggcgt gtcgacccag aacgcgcgcc aggcgtcggc gaaaccgatg tcgcctttgc
      901 gtagaatcgc cccgcaggca cgccagtcgc gcaacgccag acgcgcgccc ggcgccgcgt
      961 gcggatcacc gaacacgcgc tgttgagcgt cgggcgtgat cagcaccaga tgtcccacct
     1021 gaatccgttc gagcagagag agaaacagac ggcctgacgc ggggacggtt cggtgctcgc
     1081 tcacggttct cgaaagcgcc atggctgaat ctcctcaatc ggacgaaatg gggcgcgcag
     1141 cggcggacgc ttgaccagtt gcggcgtggt cggtggatgc gtcagtggat gcgtcggcgg
     1201 tgcggcccgg cgcaggatgt ttgccgtaga acggcgctct tttgatggcg aggcgcaacg
     1261 cttgccaatg aattcttgcg acgacgccga cagtcaggag cggctgacgg atcagcgcgg
     1321 cgagtgctgc cgaacgtgtc aagggcttta gacggccgcc gagtgctgtg cgaatcagaa
     1381 gcccgtcggc gtcccggtag tcgatcgaga ccgacgcact gttcggcgtc tctctgacgc
     1441 gaaattcgta actcccctcg acgcggcaaa acggcgacac gtgcagcact ttgcggcatt
     1501 tgagttttgt atcagctgta atcggtgcgt tgcccgcggc actcagcaga tagctgtggc
     1561 gaccgccgaa agtgttgcgc acctccgcga ggagcgcacg caaatgaccg tcgcggtcgt
     1621 ggcaaaacca gaagctgacc ggattgaacg catagcccaa tacgcgtggg aatgcttgca
     1681 gccagatcct gccgtccgcc tcttcgatac ccgcagcgga tagttgcgcg cgcatccatg
     1741 tggcaagatc gctgccgtca cgcggaccgt gatcgcgctg gcgcaggctc agcaaacgcc
     1801 aacggtcgat gccgaaccat tcacgatcga gcgacgccaa acgatccagg tcgcagcgca
     1861 cgtaaaacac gggatacgta aagcgatgat gcttcggccg cagacgctcg tgcatcacgt
     1921 tgcccgttaa aagccaggcg gccggatcga cgtcggcgcg cgggggattc atagcgtggc
     1981 ccataccggc aaagcgttga aatcggcggc cacgcgtaac gccgatttca ggccgtcttc
     2041 gtgaaaacca tagcccgtcc acgcgcccgc gaaccaggtg cggcgcttgc cttggagcga
     2101 cggcaagcgg tgctgcgcat cgatggccgc cagatcgaac agcggatggt cgtagacgaa
     2161 gcggcgcagt tcggtccccg gcgcgggctg cgtcaccgga ttgagcgtga cgacgagcgg
     2221 cgtattgaat ggcagcggct ggagttggtt gaccagatag ctgacacata ccggatgcgt
     2281 gccgtcgacg ccgcggctcc ccagataatt ccacgccgac catacgcgct gacgacgcgg
     2341 caacagattc gtatccgtat gcagcacggc gacgttcggc tggtagcgca ccgcgccgag
     2401 caccctgcgc tcgtccgggt cggcgtcttc gagcagccgc agcgtggtcg gcgcgtgggt
     2461 ggcgagcacg agcgcgtcga aacgctccgt gcctgtgtcc gtgaagacgt cgacgccgtc
     2521 ggcagcacgc cgtacgcctc tgaccgcggt ggcgatgcgg acgtcgtcga gcgtgccgac
     2581 gatacggcgc acatactcgc gccctcctcc gaccaccgtc ttccaccgtg ggcggcggtt
     2641 cacctgaagc agcgcgtggt tcaggcaaaa gcgcaggaag gtggtggccg gaaaggcgag
     2701 aatgtcggcg gtcgcgctcg accagatcgc ggcggccatc ggcaacagat agtggcgctg
     2761 gaacgcgccg ccgtagccgc cggccgtcag cagttctccc atcgacgcgc gcgtttgcgt
     2821 cgcgatctcg aggttcctct gtgcgctgct gttgaaacgc aggatgtcgc gcagcatgcc
     2881 gatgaaggtc ggcgagaaca tattgcggcg ctgcgcgaat accgtgttca ggctggtgcc
     2941 ggcccattcc aggcggcctt catcgagtga gactgaaaag gtcatatcac tcgggtgcga
     3001 ctcgacaccc agttcggcga acagcgcgac caggttcgga taggtacggt cgttgaagac
     3061 gagaaaaccg gtgtcgacgg gatgcgtgtg gccttcgagc gcgacgtcca cggtgttggt
     3121 gtggccaccc gcgtagccgg ccgattcgaa cagggtgacc tgatggttgc gcgccagcag
     3181 ataggcgctg gctaaacccg aaatcccggc gccgatgacc gcgacccgtg cgccgcatcg
     3241 tagtggagag gccggtgcgg gctgggtcat gggcgcgtct cctggtcgtg gggacgcgcg
     3301 gtcggttatc ggggtgtagc gggggttgtg cgtccttgag gggatttacg cagcggggac
     3361 tgaaacggat gcagaggtgg cggattttgc gggcgtgaag aagttaccgg tttgcctggg
     3421 tggtctcact gcgtcacatc atcccgccta tgccgcccgg actcccagtg ccgcccgtcc
     3481 cgccagagcc cgtacccgta cccgtacccg gcgatacagt aatcggatgc gccgctccca
     3541 tcgttgtcat aagcgcgacg gcggcggcgt caggctcgcc gttggcgtcg attgcaccgc
     3601 gagcgaagag cgcttcgaga tcactgtcca cgcctggctg acccacagaa aatgaggtag
     3661 tcagaaagtt gggcgcagac aacgacacac cataggtctg ttgaatattc tgtaccacag
     3721 cggcctgcgc ggtcagcagg ccgttctgat tcgccagcgt ctgctgaaac agcgtgctcg
     3781 ccgggaaacc ggcgataagc ccagtttcgt tcgtaccgag ctgcgccgcc acatacgcca
     3841 gcaacaggtc ggtttccggt gtgacgttgt acgtgccgcc ggcgaacgca accgagtgca
     3901 gcaccgtccc gctcgatgtc gcggtaatca tgcacggcat gacagcgtgg agcgtcgccc
     3961 ggtagtttcc gtttacatcc gcgccgaccg atatcgagcc ctgtgcgcaa ttgatattga
     4021 tcgtcgcgtt cgcgagcgcc ttgccggttg ctgccgtacc ggcgagcgcg accgtggaca
     4081 tgctgctagc gccggagccg cctccgcctc cgccacaagc cgcgaacaac gcaacggatg
     4141 cagtcagcag cagtccgctg gcggatcgaa tgacatgcgg ttgttgaatg ctcatggaaa
     4201 gcctgccacg ggttcggaca cgtcgtttaa acggtctgcc ggggcggtct attccatttt
     4261 tgcgcaatta acatccctac cctgcgcgtg tcgagtactt gtcgtcgacg ctgcctagac
     4321 gggcgcccgg cgcagacgtt caacgttctc ttcgcgcact gaaaaccaga gcgccaacat
     4381 gccggctgcc gccaatgcgc tcgcgagcac aaacgccgag gaataaccgg cgccgtactg
     4441 gatcatgaaa ccggtgatgc tcggcgccag aatccccgca agattggcga gcagatggac
     4501 aaagccgccg gtcgcaccga tccttccgcg cggcacgatg tcctgcagca aagaccagca
     4561 cgcctgtggc gtcatgaagg caaacaggct cgcaactgcg atcagcgaca cggcggcagc
     4621 gagcgatgtc gcgcgagata ccagcagcac gcaaacggcc gccatgccca ggcccatgat
     4681 gatcaccgtc tttcgcgcga acaggacatt tccggtgcgc ctgtaaagtc cgtcggagat
     4741 caggccgcca agcacgaatc caagcgtcgc gccgagccag ggcaggatgc cgacgatgct
     4801 catatgtttg atgtcgagat gctgatagtc ggtgaaatag ctaggcagcc acgacaggaa
     4861 aaaatacagc acgtaattga aggcgaagaa tgccatcgcg acgccgagca ccggcatcga
     4921 caacaggtaa cgcatgacgc cgtcgtctgc cgaagtgtca gaggcgttca cgacctggcg
     4981 atccgaggca atgagttggg cttcttcttg cgagaccgcc gggtgatcgg tcggttgatc
     5041 gcgaaaacaa cgccaccaga acaccatcca gacgaaaccg agcagtgcga tcacgacgaa
     5101 cgagacgcgc cagccgaact gcaaggcaac gagtccgacg atcggcgcgg cgatggccgc
     5161 gccgagtggc tggcccgcgt tcgtgatgcc aaccgcgcga cccgcctccc ggcgcggaaa
     5221 ccagttcgaa atcgacttgt tggtggtggt tcccatcggg ccttcggcga caccgaacgc
     5281 gacgcgcgcg accatcaacg tcgcgtagtt tcccgcgaac gccgtggcac cgcagaacac
     5341 ggaccagaag cctgcggcca gcatgaacac cttgcgagga ccgtagcggt cggcggccca
     5401 tccgccgatg aggcagaaca ggcaatagcc gatgaaaaag ctgctgaaca gaatgcccat
     5461 ctgcgcgtcg tcaatatgca gatcgcgttt gatgatgggt gccacgaccg acaacgcggc
     5521 gcgatccaga tagttcagca tgcccgcaaa gaacagcaag gctgcgacca tccagcggta
     5581 gtgggatttc atgtctcctc cggtgataca gaacgttgat gccccgccgg caaggaaaga
     5641 ctcccccacg tttgtgtcag cttattgaaa gtggcgttgc tggcggtgag tgcggtgctc
     5701 aggtcgtcgc atcgatgtaa ttcatacgtc atcctacaac gtaggggagc catctaacgg
     5761 cggtaattgc cctagtagcc cagatgcgat gaaaacgtta tacgataact tatgaaaaac
     5821 tgtttgcacg gtgatcgacc ggcgacgtgc agcccgcgcc ggacaggcgc agtgtgttgt
     5881 accggatagg cttacgatgg agaccctggc gggtgacgtc gtggaaagtc gcgagaatcc
     5941 ggaggcgtct tttgccgggc aggcgatcga gactccgggg gacagttttc atgtcaccta
     6001 ttcgcgagcg aagctctgtg gacctatttg acttcgccac ttctcacacg taccctgggt
     6061 tcgaagctga agggatcgaa ccatggcatg aacgatttaa acgcccccca aagctcgaag
     6121 ccccaaaaag caaaaccccc gcagagcttt cgctttgcgg gggtttttaa atttggcgga
     6181 aagggtgaga ttcgaactca cggtacccct aaaggtacac tggatttcga gtccagcgca
     6241 ttcgaccact ctgccacctt tccgggtctt ccaactcaca gcaaagtaac tgtttcgtca
     6301 gatccaagcg ggtcgttgct tgggagagaa agattataga acagctgaat ccgatttcca
     6361 agcccctcgc cagaactttt taaagcgacc tggcaagcgg taacgacatc agccgcgcat
     6421 caacccggca tcaatcaagc cgccggcact tccagccgtt ccacaccacc gaggtaagga
     6481 cgcagcgc
//
LOCUS       DATASH010000344         2432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5589393,
            whole genome shotgun sequence.
ACCESSION   DATASH010000344 DATASH010000000
VERSION     DATASH010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5589393"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1739)
                     /gene="kdpA"
                     /locus_tag="VHV99_09620"
     CDS             complement(<1..1739)
                     /gene="kdpA"
                     /locus_tag="VHV99_09620"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011352736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEX3635730.1"
                     /translation="MNSNNVLQTVVYVVVLLALAVPVSRYLTRVMEGTSRVVRFGGPL
                     ERLLYRIAGVDSSAEMGWKHYAIATIALNVLGTLFLYLLLRIQGWLPGNPQQFGPMSV
                     DGAFNTAVSFVTNTNWQDYTPEQTTGYLAQMLGFTVQNFLSAATAIAVVIALIRGFAR
                     HSAKTIGNFWVDITRVTLYVLLPMSAIIAALLMSQGVIQNFEAYQDVPTLQTTTYAAP
                     KLDAQGNPVKDAKGNAVTVDTKVTTQTIAMGPVASQEAIKMLGTNGGGFFNGNSAHPF
                     ENPTPFSNFVQMLAILVIPAALALVFGRMIADRKQGIAVLAAMTIAFTACVVGEISAE
                     QAGNPTLTALHVDQSANALQSGGNAEGKETRFGIAQSGIFTVATTAASCGAVANTHDS
                     LTPIGGLYPMLLMQLGEVIFGGVGSGMYGMLVFALLAVFVAGLMIGRTPEYVGKKIEA
                     YEMKMVSIVVLLTPLLVLVGTSIAVLSEAGRAGILNPGAHGFSEILYAFSSAANNNGS
                     AFAGLTVSTPFYNWLTAIAMWFGRFGTIVPVLAIAGSLAAKKRIGVTAGTLPTHGPLF
                     VVLLLGTVLLVGAL"
     gene            complement(1736..1828)
                     /gene="kdpF"
                     /locus_tag="VHV99_09625"
     CDS             complement(1736..1828)
                     /gene="kdpF"
                     /locus_tag="VHV99_09625"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017331558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HEX3635731.1"
                     /translation="MTWILWLSGATTALLFAYLVYALLRAEDIE"
     gene            complement(1825..1938)
                     /locus_tag="VHV99_09630"
     CDS             complement(1825..1938)
                     /locus_tag="VHV99_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007583456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium ABC transporter ATPase"
                     /protein_id="HEX3635732.1"
                     /translation="MDVVYVGGIVLFAALTFALISGCEKLIQFRRGHGARS"
BASE COUNT          499 a          759 c          746 g          428 t
ORIGIN      
        1 agcgcgccga ccagcagaac ggtgccgagc agcaggacga caaacagcgg gccgtgcgtc
       61 ggcagcgtgc cggcggtcac gccgatgcgc ttcttcgcgg cgagcgagcc ggcgatcgcg
      121 agcaccggga cgatcgtgcc gaagcggccg aaccacattg caatcgcggt gagccagttg
      181 tagaacggcg tgctgacggt gaggcccgcg aatgcgctgc cgttgttatt ggccgccgag
      241 ctgaacgcgt agaggatttc ggagaaaccg tgcgcgccag ggttgagaat gccggcccgg
      301 cccgcctcgc tcaacaccgc gatcgacgtg ccgaccagca ccagtagcgg cgtgagcagc
      361 acgacgatcg acaccatctt catttcgtac gcctcgatct tcttgccgac gtattccggc
      421 gtgcggccga tcatcagccc ggcgacgaac accgccagca acgcgaacac cagcatcccg
      481 tacatgcccg aacccacgcc gccgaagatc acttcgccca gttgcatcag cagcatcgga
      541 tagaggccgc cgatcggggt gagcgagtca tgcgtattgg cgaccgcgcc gcacgaagcc
      601 gccgtggtgg cgaccgtgaa gatgcccgac tgcgcgatgc caaagcgcgt ctccttgcct
      661 tccgcgttgc cgcccgactg caatgcattc gccgattggt cgacgtgcaa cgcggtcagg
      721 gtcggattgc cggcctgctc cgcgctgatt tcacccacca cgcaagcggt gaacgcaatc
      781 gtcatcgcgg ccagcacggc gataccctgc ttgcggtccg caatcatccg gccgaacacc
      841 agcgcgagcg ccgccggaat cacgaggatc gcgagcatct gcacgaaatt cgagaacggc
      901 gtggggttct cgaacggatg cgctgagttg ccgttgaaga agccgccacc gttggtgccg
      961 agcatcttga tcgcttcctg cgacgccacc gggcccatcg cgatggtctg cgtcgtcacc
     1021 ttggtgtcga cagtcaccgc attacccttc gcatccttca ccgggttgcc ttgcgcgtcg
     1081 agctttggcg cggcataggt ggtggtttgc agcgtcggca cgtcctgata ggcctcgaag
     1141 ttctggatca cgccctggct catcagcaag gcagcgatga tggcagacat cggcagcaac
     1201 acgtacagcg tcacgcgcgt aatgtcgacc cagaagttgc cgatggtctt cgccgaatgg
     1261 cgtgcaaaac cacggatcaa cgcgatcacc actgcgatgg cggtggctgc ggacaggaag
     1321 ttctgtaccg tgaagccgag catctgcgcc agataaccgg tggtctgttc aggcgtgtaa
     1381 tcctgccagt tcgtgttggt cacgaagctg acagccgtat taaaggcgcc gtcgaccgac
     1441 atgggaccga actgctgcgg attgcccggc agccagcctt gaatacgcaa cagcaggtac
     1501 aggaacagcg tgccgagtac gttcaacgcg atcgtggcga tcgcgtagtg cttccagccc
     1561 atctcggccg acgaatcgac gcccgcgatg cgatacagca agcgctcgag cggaccgccg
     1621 aagcggacca cacgagaggt accctccatc acgcgcgtga gataacgcga aaccggcacg
     1681 gcgagtgcaa gcaacacgac cacgtaaacg accgtttgca ggacattgtt cgaattcatt
     1741 cgatgtcctc cgcgcgcaac agcgcataga cgaggtaagc gaacagcagc gcggtagtag
     1801 cgcctgatag ccagaggatc caggtcatga gcgtgctccg tggccacgcc ggaactggat
     1861 cagcttctcg cagccggaaa tcaaagcaaa ggtcagcgct gcgaatagca ctattccccc
     1921 gacgtacacc acatccattt tgtgttctcc agtagcggac tttggatcgg tcgaaggtta
     1981 tgccgatctg cgtaaaggcc tggtcaaaga tggcaggggc gttataaaaa atgcgtaaac
     2041 aaagagggcc gctcccgctg ggtacggaac ggcctcattc cgtggagact gactttacgg
     2101 gtatttcccg caagcgcgcg gcggcgccgc aggcgcggga ttttgataat gttgttactg
     2161 cgctttgccc tgtttcccgc gacgcgcgcc agcatgccac cgcgcgcatg agggccttac
     2221 gggcgggtcc ggcgagttcg cagctggcgc ccgttcaagc gatagtatcc agcagatcca
     2281 gtaacgcgtc cccgtcgaca ggctttctga agaagccgag tgcgcctcgc tgcagataga
     2341 agtcttccgt atgtccacct ggtcgccccg tgataatgat gaccggcgtc gctgcgtccg
     2401 aatgaaccgt tctgtcgagc acctgaatgc cg
//
LOCUS       DATASH010000345         4989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4795864,
            whole genome shotgun sequence.
ACCESSION   DATASH010000345 DATASH010000000
VERSION     DATASH010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4989
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4795864"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            410..1063
                     /locus_tag="VHV99_09635"
     CDS             410..1063
                     /locus_tag="VHV99_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017332930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEX3635733.1"
                     /translation="MNFEQARFNMIEQQIRPWEVLDQDVLNLLSIVKRENFVPTIYRD
                     LAFVDFEVPLPAGQHMLAPRVEARVLQELAVKKHESVLEIGAGSGYMAALLAHRAQHV
                     LTVDIEPELAELAKHNLIANGVLNAEVATGDASRGWANAAPYDVICVSGGLPVLPQEI
                     LEHLKIGGRLAAFVGTAPVMKAQIITRIDEKQYRIADVFETYVEPLINAVQPPRFKF"
     gene            1145..1468
                     /locus_tag="VHV99_09640"
     CDS             1145..1468
                     /locus_tag="VHV99_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010091617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX3635734.1"
                     /translation="MQNLTAPALAEWLADTSRPAPVLLDVREPWEIQTASIAGAVSIP
                     MREIPARSEELDDDAQIVCVCHHGARSAQVAMFLESRGHTSVFNLQGGIDAWSRQVDP
                     SVPTY"
     gene            2158..2631
                     /locus_tag="VHV99_09645"
     CDS             2158..2631
                     /locus_tag="VHV99_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2844 domain-containing protein"
                     /protein_id="HEX3635735.1"
                     /translation="MRFLKTMMLGATLFPLASYAMLGGAPSAGASSSAMVLRATSHSV
                     AAAGTAETTATAPYTLRESRDANAVTIREYVLPNNVVFAVAWEGPIRPDMTALLGSYF
                     PNYVAAGESRARGTGPLFESNDDFHIESAGRLGHFFGMAYLPHLMPANVRPADLK"
     gene            2664..3965
                     /locus_tag="VHV99_09650"
     CDS             2664..3965
                     /locus_tag="VHV99_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3443 domain-containing protein"
                     /protein_id="HEX3635736.1"
                     /translation="MTTINKTLWIALVALGLTLSACGGGSDGNGSKNNGSIAWSADPD
                     WITKPDGSGSSDSSGNTSSSGGNTVPIRVDNSMGSINMLYATITVCVPGTQGTSQCKT
                     VDRMLVDTGSVGVRIMASALPTLRSQLLTQVGAVDDVSGVAPIAECMPFGSGTTWGSV
                     KRADVKIGSRTASNIPIQLVGDGAYAIPSDCVAHGGPDLSTVEKLGANGILGIGYGTQ
                     DSKDALTTALPGNYYYCTGANACFNTRMTAGKEVINPVAAFSSDNNGTIIRLPKLSAG
                     GQASVTGELVFGIGTQSNNALPSNVNVLALDEYGEFTTQYQGQAFNWSAIDSGTNGFA
                     FQDDSIPTTSGWYTPSVALNLAATMEATSGKGTPVNMPFAIDSAVRVSANGYAAYDNV
                     GWYQSSSRMFMWGLPFFYGRSVYTTVGIGTIGKQSGPFVAF"
     gene            4144..4593
                     /locus_tag="VHV99_09655"
     CDS             4144..4593
                     /locus_tag="VHV99_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2844 domain-containing protein"
                     /protein_id="HEX3635737.1"
                     /translation="MKFVKIVLAVSALLPLASYAALGAAPGAGSAPQTLLRAATSNSS
                     SSAAAYSVRESRDADGVRIREYVLPSNVVFAVTWQGPVRPDMVALLGSYFPNAVSAGT
                     AHVRGTGPLIERNGDFQIESAGRAGNFFGKAVLPRLVPANVRADDLQ"
     gene            4625..>4989
                     /locus_tag="VHV99_09660"
     CDS             4625..>4989
                     /locus_tag="VHV99_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635738.1"
                     /translation="MNTLNKKLCFALLTASLALSACGGGGGDDGSSNATKDNDNSATP
                     TPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTEVVVHGPTRKHDV
                     ADTTVSAAQASKVVGTQGDP"
BASE COUNT          950 a         1624 c         1487 g          928 t
ORIGIN      
        1 gcgccttgga cgtcgatctg gacaagtgac ctcgcgtcga aaatgtaatc gattacattt
       61 ttgagcgatc aacgcgcaaa tcgcaagtac ggatcgctag ggattaacac ggggtcgcgc
      121 cgcgacgcga caggccatgc acagccgcac gcgctcgccg tcagtcgggg ccatcctgcc
      181 cgcgacggca cggctggccg ccgccatttg gtacaatccc gcagttaccc tggcgtggct
      241 tgcgtcccgc atcgttggag tctcgttcgc gaggttcggg cgagcttcgg gaagctgccg
      301 gccggaggca aaaaagtcag tggcggcgtt tgccaaccgc atgcaagcgc atcgtcaaac
      361 gcctcgtcga actgccaccc actcgccaaa ccaaaccgcc gaatccacca tgaacttcga
      421 gcaagcgcgt ttcaacatga tcgaacagca gatccgcccc tgggaagtgc tcgaccagga
      481 cgtgctgaat ctgctctcga tcgtcaagcg tgaaaacttc gtccccacca tctaccgcga
      541 tctggccttc gtcgacttcg aagtgccgct gccggctggc cagcacatgc tggcgccgcg
      601 tgtcgaagcg cgcgtgctgc aggaactggc ggtgaagaaa cacgaaagcg tgctcgaaat
      661 cggcgctggc tcgggttaca tggcggccct gctcgcgcac cgcgcgcagc acgtgctgac
      721 ggtcgacatc gaaccggaac tggctgaact ggcgaagcac aacctgatcg ccaacggcgt
      781 gctgaacgcc gaagtcgcca cgggcgacgc ctcgcggggc tgggccaacg ccgcaccgta
      841 cgacgtgatc tgcgtatcgg gcgggctgcc ggtgctgccg caggaaatcc tcgaacacct
      901 gaagatcggt ggccgtctgg cggcctttgt cggcaccgcg ccggtcatga aggcgcagat
      961 catcacgcgt atcgacgaaa agcaataccg tatcgccgac gtgttcgaga cgtacgtcga
     1021 gccgctgatc aacgctgtgc agccgccgcg cttcaagttt taagtcgcac ctgcgcgcgt
     1081 ttcgttgcgc acgtttatct gtacggccgg cacaccgccg tccgctgaac cggatgtcct
     1141 gctgatgcaa aatctgaccg ctcccgcgct cgccgaatgg ctcgccgaca catcgcgccc
     1201 cgcgcccgtg ctgctcgacg tgcgcgaacc gtgggaaatc cagacggcgt cgatcgccgg
     1261 cgccgtgtcg attccgatgc gtgaaattcc agcgcgcagc gaagagctcg acgacgacgc
     1321 gcaaatcgtc tgcgtatgcc accacggcgc gcgcagcgcc caggtcgcga tgttcctcga
     1381 atcgcgcggc cacaccagcg tgttcaatct gcaaggcggg atcgacgcgt ggtcgcgtca
     1441 agtcgatcct tccgttccca cctattgatc gaacggtcac gatgatgcgc cagcgggtgc
     1501 cggtccgcgc ggccgcgcgg gcgctcattg cgtatttcat ggcgcgttcc gtggcggtcg
     1561 tgtctgcgca tctgttgccg cactcgcggc gctgatcccg ctgccggccc aggcggtcgg
     1621 cctctcccag ttctacgacg aagccctctc caacgacccg cagctgcaaa gcaagcgcgc
     1681 ctcgcgctgc tgtcgccgct cgacgatggt tcgctgaccg cgctgccgca agcgccgacg
     1741 gtgccgcatc cggtcctcgc tgcgaaacgc gctgcaggga aggccgggcg gcgggctatg
     1801 ccgagcacgt tggcgcctcc gcaggctcag tagtacttcg cttactgctt ttcctttcca
     1861 aacgctcccg ccggattcga tcggctgttt ccgtcagcta tccgcgcgct gttcgaagcg
     1921 ccaccacttc gaacagcgcc gttacctttt cccccatcga tacagatcgt caactcatcg
     1981 cgatcgagcc cttgtcttaa gtcatgtcaa cgtcggcctc ggcaattaag acgacttaag
     2041 acaacgctga caccgcgctt tcaataagcc gcttctcgtc actgccccgg aaaattctta
     2101 cggtttcctg aacacctcgc gggcaacatg ctgcgacaag tttttcggag ccgtgtaatg
     2161 agatttttga agaccatgat gcttggagca acgctgtttc cactcgcatc gtatgcaatg
     2221 ctgggcggcg cgccgagcgc cggtgcatcc tcctctgcaa tggtactgcg cgccacgtcg
     2281 cactctgtcg ccgcggccgg tacggcggaa acgaccgcca ccgccccata caccttgcgc
     2341 gagtcgcgtg acgccaacgc tgtcacgatc cgtgaatacg tgctgccaaa caatgtcgtg
     2401 ttcgccgtcg cgtgggaagg acccatccgt ccggatatga cggcgctgct cggcagttat
     2461 ttcccgaact atgtggctgc cggcgaaagc cgcgcgcgcg gcaccggtcc gctgttcgaa
     2521 agcaacgatg acttccacat tgaatcagcc ggccgcctgg ggcacttctt cggcatggca
     2581 tatctgccgc atctgatgcc ggccaatgtc cgcccagccg atctgaagta atacggcgta
     2641 tcgatttcgc ggaaaacaac aagatgacga caatcaacaa gacactctgg atcgcgctgg
     2701 tagcgcttgg cctgacgctg tccgcgtgtg gcggcggaag tgacggaaac ggcagcaaga
     2761 acaatggctc gatcgcctgg tcggccgatc ccgactggat cacgaagccg gatggctcgg
     2821 gcagttcaga tagttcgggg aatacgtcca gcagtggcgg caacacggtg ccgattcgag
     2881 tcgacaactc gatgggcagc atcaacatgc tttacgcaac gatcaccgta tgcgtgcccg
     2941 gcacgcaagg cacgagccag tgcaagaccg tcgatcgcat gctcgtggat actggctcgg
     3001 taggtgtacg catcatggcc agcgcattgc cgacgctccg gtcccagttg ctgacgcaag
     3061 tcggcgccgt cgacgacgta agcggcgtcg caccgatcgc cgaatgcatg ccgttcggtt
     3121 ccggcaccac ctggggctcg gtgaaacgcg ccgacgtcaa gatcggcagc cgcacggcaa
     3181 gcaatattcc catccagctg gtcggcgacg gcgcgtatgc cataccgtcg gactgcgtcg
     3241 cgcatggcgg cccggacctc agtaccgtcg agaagctcgg cgccaacggt attctcggca
     3301 tcggctacgg cacccaggat tcgaaagacg cgctgaccac cgcgctgccg ggcaattact
     3361 actattgcac gggcgctaac gcgtgcttca acacgcgcat gacggccggc aaggaggtga
     3421 taaaccctgt cgccgccttc tcgagcgata acaacggcac gatcatccgc ctgcccaagc
     3481 tgtcggcggg aggccaggcg agtgtcaccg gcgaactggt attcggtatc ggtacgcaat
     3541 cgaataacgc gttgccgtcg aacgtgaacg ttctggcgtt ggatgagtac ggcgaattta
     3601 cgacccagta tcaaggtcag gcgttcaact ggagtgcaat cgacagcggt accaacgggt
     3661 ttgcgttcca ggacgattcg attcccacta cgtcgggctg gtacacgccg tcagtcgcgc
     3721 tcaacctggc tgccacgatg gaggccacca gcggcaaggg aacgcccgtc aatatgcctt
     3781 ttgcgatcga cagtgccgtc cgcgtgtcgg cgaacgggta tgccgcgtac gacaacgtcg
     3841 gctggtatca gtccagttcg agaatgttca tgtggggtct gcccttcttc tacggacgca
     3901 gtgtgtacac gacggtcggc atcggtacga taggcaagca gagcggaccg tttgtcgcgt
     3961 tctgatggaa tgggtgtgac ttattcggga ggcgacgtcg cgaggcgcct ccctcccgcc
     4021 ttctgccgca gcacaaatcg atcgagtcgc atcggtgttt cctactcacc gttgagattt
     4081 ttcagagact ctcaataatc acccgctggc aacatgctcc gacctgtttt ttggagcaat
     4141 gtgatgaaat ttgtaaagat cgtgctggcg gtgtcggctc ttttgccgct cgcgtcgtat
     4201 gcggcgttgg gtgctgcgcc gggcgcgggg tcggccccgc agacgttgct tcgggcggca
     4261 acctctaatt cctcttcttc tgccgcggcg tacagcgtgc gtgagtcgcg tgacgctgac
     4321 ggtgtccgga ttcgcgaata cgtgttgcct agcaacgtcg tttttgccgt cacgtggcaa
     4381 gggcctgtgc gcccggacat ggtcgcgctc ctcggcagct acttcccgaa cgcagtctcc
     4441 gcagggacag cgcacgtgcg aggcaccggt ccgttgatcg agcgcaacgg cgactttcag
     4501 atcgagtcgg cgggtcgcgc cggcaacttc ttcggcaagg cagtcctgcc gcgcctcgta
     4561 cccgccaatg tccgtgctga cgatctgcag tgacgcgaca catcgatcac ggaacacaag
     4621 aaacatgaac acgctcaata agaaactttg cttcgcgctg ctgacggcga gcttggccct
     4681 ttcagcctgc ggtggtggtg gcggcgacga cggcagctcg aacgcgacga aggataacga
     4741 caactcggca acgccgacgc ccacgccgac gccgactccg acacccacgc cgactccgac
     4801 tccgactccg actccgactc caacacccac tccgactccg actccgaccc cgactccgac
     4861 cccgaccccg accccgaccc cgaccccgac cgaggtcgtc gttcacggcc ctacgcgcaa
     4921 gcacgacgtt gccgatacga cggtcagtgc agcccaggcg agcaaggtgg tcggcacgca
     4981 gggagaccc
//
LOCUS       DATASH010000346         7223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4204235,
            whole genome shotgun sequence.
ACCESSION   DATASH010000346 DATASH010000000
VERSION     DATASH010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7223
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4204235"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /locus_tag="VHV99_09665"
     CDS             complement(<1..951)
                     /locus_tag="VHV99_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX3635739.1"
                     /translation="MDSFYTDEQRMIRDAARDFATERLAPYAGQWDRDAKLPAEVVTQ
                     MGELGFLGMIVPSEWGGSYTDYVAYALALEEIAAGCAACATLMSVHNSVGCGPILNFG
                     TDAQKDRYLQDLATGRRIGAFCLTEPQAGSEANNLRTRAVLRDGKWILNGNKQFVTNG
                     ARANIAIVFAVTDPDRGKRGLSAFIVPTDTPGFNVGKPEHKLGIRASDTCPISLDDCA
                     VPEANLLGEPGEGLRIALSNLEGGRIGIAAQAVGIARAAFDAARRYANERIQFGKALK
                     DHQTIANMLADMATRLNAARLLVHHAARLRTAGRPCLSEAS"
     gene            1295..2341
                     /locus_tag="VHV99_09670"
     CDS             1295..2341
                     /locus_tag="VHV99_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX3635740.1"
                     /translation="MKNLKNDKGTISVSLVEETLALARARGFDALPLAEAAGIAGPLL
                     ASPKSRVSSAQYGALWAGIARALDDEFFGQDSHRMKSGSFIAMTQTALTARNGAQALA
                     RAVRFMRLVLDDLSAQIETDAGRVRLRFVEREGIKPPAMFAYATYFILVYGLLCWLVG
                     RRIPLLEARFRCAEPPAAHEYRLMFCDDMTFDQAESYVDLSPAFLELPVIQTAKSVKP
                     FLRDAPGSFIVKYRNPGSLAARVRKMLRALPMSGWPAADQMAERLHVAEATMRRRLKQ
                     EGYTYQSIKDDLRRDIAIGELQDTDRTIADIATSVGFAEPSAFHRAFRKWTGMRPTDY
                     RTARADFTRRPESD"
     gene            2424..4106
                     /locus_tag="VHV99_09675"
     CDS             2424..4106
                     /locus_tag="VHV99_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HEX3635741.1"
                     /translation="MSTYPRLPFRQSGPRPALIRGQGVGRLMRGMTAFGVLLALGFAL
                     CENAPRGWVAEAAQAAVAPNSVVVIPVNGAISPASADFIVRSLQRAAEDRAQLAVLQL
                     DTPGGLDTSMRQIIKAILGSAVPVATFIAPSGARAASAGTYIVYASHIAAMAPGTNLG
                     AATPIQMGIGGAEPPAGGGMPGLPGTGGGGGAGAQKTEPGVPASAGSVKSASSTSSAS
                     SANPADSSASAPSGALPLDTQATELRKQVHDAAAYIRGLAQMRGRNAVWAERAVREAV
                     SLSASEALAQHVVDLNARDIPDLLRQLDGRTIGTSHGDVKLSTANAPVVTLEADWRSQ
                     FLAVITDPNVALVLLMIGMYGLFFEFANPGFVLPGVVGAISLLMGLFAMQMLPVNYVG
                     LGLIFLGIAFLIGEAFLPTFGSLGFGGVVAFVIGALMLIDTDVPGYGIPLPLIAAVVV
                     FSVAFVFGVSRLALHARRKPVVTGSEGLIGSVGVVLDGGLLPEGGLLPGDAAADATAG
                     GTLAGWAQVHGERWRVSSTAPVAAGHAVRVTARRGLTLTVVPAETRQQGERS"
     gene            4103..4876
                     /locus_tag="VHV99_09680"
     CDS             4103..4876
                     /locus_tag="VHV99_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014971933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEX3635742.1"
                     /translation="MIGFTFGFSSILILLVIALIASSIRIFREYERGVVFMLGRFWKV
                     KGPGLVLIIPIVQQAVRMDLRTVVFDVPPQDVITRDNVSVKVNAVVYFRVVDPEKAVI
                     QVARYFEATSQLSQTTLRAVLGKHELDELLADREQLNADIQKVLDAQTDAWGIKVSIV
                     EIKHVDINETMIRAIARQAEAERERRAKVIHAEGELQASQHLLEAAQTLAKQPQAMQL
                     RYLQTLTTIAADKNSTIVFPLPIDLLSAVLDRFSKPSPS"
     gene            5014..5271
                     /locus_tag="VHV99_09685"
     CDS             5014..5271
                     /locus_tag="VHV99_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4148 domain-containing protein"
                     /protein_id="HEX3635743.1"
                     /translation="MKIVSLVGLGALLAAVAMNGAFAQTSRPYDPSAPKTRAEVKADL
                     AEWLAAGYDPLDWIDYPQNAQRAGRIVAARRAQQADGSMTQ"
     gene            5778..7079
                     /locus_tag="VHV99_09690"
     CDS             5778..7079
                     /locus_tag="VHV99_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEX3635744.1"
                     /translation="MDAIKRYFGFEAAGTNLRTEVLAGLTTFLTMAYIIFVNPAILGD
                     AGMPKDAVFVATCIVAALASLIMGLYANYPIALAPGMGLNAYFAYTVVKGMGFTWQAA
                     LGAVFISGCLFLIVTLFRVREVIVNGIPHSIRIAITGGIGLFLAIISLKSAGVVVGNP
                     ATLVTLGDLHNPHAILAVIGFFAIVTLDYLRVRGAILIGIVGVTVLSFFFGGNQFHGI
                     VSAPPSIAPTLFQLDIRGALSGGVLNVILVFFLVELFDATGTLMGVANRAGLLVEGKM
                     HRLNRALLADSTAILAGSMLGTSSTTAYIESASGVQAGGRTGVTAITVAILFLAALFF
                     APLAGVVPGYATAPALLYVSCLMLREMLDLPWDDATEVVPAALTALLMPFTYSIANGV
                     AFGFISYAGLKLLTGQARKVKLVVWIIAAIFLFRYFYLGSE"
BASE COUNT         1161 a         2334 c         2356 g         1372 t
ORIGIN      
        1 cgaggcctcg gacaaacacg gccggcccgc cgtgcgcagc cgcgccgcat gatgcacgag
       61 caggcgcgcg gcgttcagcc gggtcgccat gtcggcgagc atgttggcga tggtctggtg
      121 atccttcagc gccttgccga attgaatgcg ttcgttcgcg taacggcgtg cggcgtcgaa
      181 tgcggctcgc gcgatgccga ccgcctgcgc cgcgatacca atgcggccac cctcgagatt
      241 cgacaacgcg atgcgcaggc cttcgcccgg ttcgccgagc agattggctt cgggcaccgc
      301 gcaatcgtcg agcgagatcg ggcaggtgtc cgaggcgcga atgccgagct tgtgttcggg
      361 tttgccgacg ttgaagcccg gcgtatcggt cggcacgatg aaggctgata gaccacgctt
      421 gccgcggtca ggatcggtca cggcaaacac gatcgcgata ttggcgcgcg cgccgttggt
      481 cacgaactgc ttgttgccgt tgagaatcca tttgccgtcg cgcagcaccg cgcgggtgcg
      541 caggttgttt gcctcggagc cggcttgcgg ctcggtcagg cagaacgcac cgatgcgccg
      601 gcccgtggcg aggtcttgca gatagcggtc tttttgcgcg tcggtgccga agttcaggat
      661 cggcccgcag ccaaccgagt tgtgcacgct catcagcgtg gcgcaggcgg cgcagcccgc
      721 ggcgatttct tcgagcgcga gcgcataggc cacgtagtcg gtataggagc cgccccactc
      781 ggacggcacg atcatgccga gaaagccgag ctcgcccatt tgcgtgacca cttccgccgg
      841 cagcttcgcg tcgcgatccc actgcccggc gtacggcgcg aggcgttcgg tggcgaagtc
      901 gcgggcggcg tcgcggatca tgcgttgttc gtcggtgtag aagctgtcca tgggctcggt
      961 cctgttatgt gcagcgagtc gcggcagcga ggcgcatgcg cctcgactcg cggtggcgcc
     1021 acctcatcat ccgcggttcg tcgcgggggc gtgatcggcc tcttcgcgac cttgcagcgg
     1081 cctcgccgtg tgtctccggg cgatgcggtc cgcgatcggt tgatgccaag tgtagggaag
     1141 cggcaggaca ggttcgatgc tcagcccgat cattctggtc gagcgcattt gccatggcgt
     1201 tatcatgtca acccggttcg ggccgcattt tcaaaggctt agcggatcgt cacaaggaaa
     1261 atgttgagcg cgtttgccag accgtcacga aaatatgaag aacctcaaga acgataaggg
     1321 cacgatttcc gtcagtctgg tcgaggagac cctcgcgctg gcacgcgcgc gcggtttcga
     1381 tgcgctgccg ctcgccgaag ccgccggcat tgcgggaccg ctgctggcgt cgccgaagag
     1441 ccgcgtgtcg tcggcgcagt acggtgcgct gtgggccggc atcgcccgcg cgctggacga
     1501 cgagttcttc ggccaggact cgcaccgcat gaaaagcggc agcttcatcg cgatgacaca
     1561 aacggcgctg accgcgcgca acggcgcgca agcgctcgcg cgtgcggtcc gcttcatgcg
     1621 cctggtgctg gacgatctaa gcgcgcaaat cgaaacagat gccgggcgcg tacggctcag
     1681 gttcgtggaa cgcgaaggca tcaagccgcc cgcgatgttc gcctatgcga cctacttcat
     1741 ccttgtctat ggcctgttgt gctggctggt cgggcggcgc attccattgc tcgaggcgcg
     1801 ctttcgctgc gccgagccgc ctgccgcgca tgaataccgt ctgatgttct gcgacgatat
     1861 gacctttgat caagcggagt catatgtcga cctgtcgccg gcgtttctcg aactgcctgt
     1921 gatccagacc gccaagtcgg tcaagccgtt tctgcgcgat gcgcccggca gcttcatcgt
     1981 caaataccgc aatcccggct cgctcgccgc gcgcgtgcgc aagatgctgc gcgcgctgcc
     2041 gatgtccggg tggcccgccg ccgaccagat ggcggagcgc ctgcatgtcg ccgaggccac
     2101 catgcggcga cgtctgaagc aggaaggcta tacgtatcag tcgatcaagg acgacttgcg
     2161 gcgcgacatc gcgatcggcg aactgcagga caccgatcgt acgatcgccg acatcgccac
     2221 ctcggtgggt ttcgccgagc cgagcgcgtt tcaccgcgcg tttcgcaaat ggaccggcat
     2281 gcggccgacc gattaccgga cggcgcgtgc ggatttcacg cgtcgcccgg aatcggacta
     2341 agctgtaagg agcaggccag cctatttggg gtatcggccc gcgcctgccg atacccgtcg
     2401 gcatggcgcg gcagccgtca gcgatgagca cgtatccgcg tctcccgttc cggcagtctg
     2461 gtccgcgccc tgcgcttatc cgtggccaag gtgtgggccg gttgatgcgc ggcatgaccg
     2521 cgttcggcgt actgttggcg ctcggcttcg cattgtgcga aaacgcgccg cgcggttggg
     2581 tcgccgaggc cgctcaggcc gccgtggcgc cgaacagcgt tgtcgtgatt cccgtgaacg
     2641 gcgcgatcag tccggccagc gccgacttca tcgtgcgcag cctgcaacgc gccgccgaag
     2701 atcgcgcgca actcgccgtg ctgcaactcg atacgcccgg cgggctggat acgtcgatgc
     2761 ggcagatcat caaggcgatt ctcggctcgg cggtgccggt cgccacgttt atcgcgccca
     2821 gtggtgcgcg cgcggcgagc gccggcactt atatcgtcta cgccagccat atcgcggcca
     2881 tggccccggg caccaatctc ggcgcggcga cgccgatcca gatgggcatc ggcggtgcgg
     2941 agccgccggc gggcggcggc atgcctggat tgccgggcac gggaggcgga ggcggcgctg
     3001 gcgcccagaa gaccgagccc ggcgtgcctg cctccgccgg ttcggtgaag tcagccagtt
     3061 ccacgagctc agccagctca gcgaacccag ccgactcgtc cgcgagcgcg cccagcggcg
     3121 cccttccact cgacacccaa gccaccgaac tgcgcaagca ggtccacgac gcagccgcct
     3181 acatccgcgg cctcgcgcaa atgcgcggac gcaacgccgt ctgggccgaa cgcgccgtgc
     3241 gcgaagccgt cagcctgtcg gcgagcgaag ccctcgcgca gcacgtggtc gatctgaacg
     3301 cacgcgacat tcccgacctg ttgcgccagc tggacggccg cacgatcggc acgagccacg
     3361 gcgacgtcaa actcagcacc gccaacgcac cggtggtcac gctcgaagcc gactggcgca
     3421 gccagttcct cgcggtcatc accgacccga acgtcgcatt ggttctgctg atgatcggca
     3481 tgtacggcct ctttttcgag ttcgccaatc cgggcttcgt actgccgggc gtggtcggcg
     3541 ccatcagtct gctgatgggg ctcttcgcga tgcagatgct gccggtcaat tacgttggac
     3601 tcggcctgat ctttctgggc atcgccttcc tgatcggcga ggcgtttctg ccgactttcg
     3661 gctcgctcgg cttcggcggg gtggtcgcct tcgtgatcgg cgcgctgatg ctgatcgata
     3721 ccgacgtgcc cggctacggc attccgctgc ccctgatcgc ggccgtggtc gtgttcagcg
     3781 tcgcgtttgt gttcggcgta tcgaggctcg cgttgcacgc gcggcgcaag ccggtggtga
     3841 ccggctcgga agggctgatc ggcagtgtcg gcgtcgtgct cgacggcggc ctgctgccgg
     3901 aaggcggcct gctgccgggg gatgccgcgg cagacgcaac agcgggcggc acgctggccg
     3961 gctgggcgca ggtgcacggt gaacgctggc gggtgtccag cacggccccg gtcgcggccg
     4021 gtcacgcggt gcgcgtcacg gcgcggcgcg gactgacgct gaccgtggtg cccgcggaaa
     4081 cacgacaaca aggagaacgc tcatgatcgg tttcacattc ggcttcagca gcattctgat
     4141 tctgctggtg atcgcgctga ttgcctcatc gatacggatt tttcgcgagt acgaacgcgg
     4201 cgtggtgttc atgctcgggc gcttctggaa ggtcaagggg cccggcctcg tcctgatcat
     4261 cccaatcgtc caacaggcgg tgcgcatgga tttgcgcacc gtcgtgttcg acgtgccgcc
     4321 acaagacgtg atcacgcgcg acaacgtttc ggtgaaggtc aacgcggtgg tgtatttccg
     4381 cgtcgtcgat ccggagaagg ccgtgatcca ggtggcgcgt tatttcgagg cgaccagcca
     4441 gctatcgcaa accacactgc gcgcggtgct cggcaagcat gaactcgatg aactgctggc
     4501 cgatcgcgaa caactgaacg ccgatatcca gaaggtgctc gatgcgcaaa cggatgcgtg
     4561 gggtatcaag gtgtcgatcg tcgagatcaa gcatgtggac atcaacgaaa caatgatccg
     4621 tgccattgcc cgtcaggccg aagccgagcg tgaacggcgc gccaaggtga ttcatgcgga
     4681 aggtgaattg caagcctcgc agcatctgct ggaggcggcg cagacgctcg cgaagcagcc
     4741 gcaggccatg cagttgcgtt acctgcaaac cctcacgacg attgccgccg acaagaattc
     4801 gacgatcgtg tttccgctgc cgatcgatct gctgagcgcg gtgctggatc gcttcagcaa
     4861 accgtcgccg tcctgaatcg ggatcgcgcg ggttcaggcg gggccgcatt gaatcacggg
     4921 atgaaacggt gcattacctt caaccgcctt actcgaaagg ctgacgaaaa ttaacatccc
     4981 gaggtgtttt taaccgctcc ataggagctc atcatgaaaa tcgtttcact cgttggactc
     5041 ggcgcgctgt tggccgccgt tgccatgaac ggcgcgtttg cgcagacctc gcggccttac
     5101 gacccgtcgg cgccgaaaac gcgtgccgag gtgaaagccg atctggccga atggctcgcc
     5161 gccggctacg atccgctcga ctggatcgac tatccgcaga atgcacaacg cgcgggccgc
     5221 attgtcgccg cgcggcgggc gcagcaagcg gacgggagca tgacgcagta agcagtttca
     5281 tcgtcgtctt gaagcggcat gccgggccgc ccgcgaccgc aagggtcgcc ggcgacgtgg
     5341 gtcttgcatg cccgcaacct gggcgtcagt catctgcctg tcgctcgccg ctcgggctcc
     5401 gcgcgatttt ttgcggttag cttctgcccg aatggtttca atccacgcca ttccgccagg
     5461 ctctgccgct ctccgttccc gcgcaaaccc accgttcccg gccgtaacat cgtaaattcg
     5521 cttgccgcgc cgccccgcgc gctggcaagg tatcatctcg ccgcttcagc caggctccca
     5581 gtgacacgat agaggcggcg ccgtcgatcg taatgacaat ccgcctgaca agccgcccgt
     5641 gggcctggct gcgcggcgca tcggtgccat gccattgcgt gccgtgcggg acgggaaggt
     5701 aagtcgcccg cctgttagct gcgcattgcc cgtatcaacc acaagaaacg cattttgaac
     5761 aacgagagac ccgcctcatg gacgccatca aacgatactt cggcttcgaa gccgccggca
     5821 ccaacctgcg caccgaagtg ctcgccgggc tgaccacctt cctgacgatg gcctacatca
     5881 tcttcgtcaa cccggcgatt ctcggcgacg ccggcatgcc gaaggacgcg gtgttcgtcg
     5941 ccacctgcat cgtggccgcg ctggcctcgc tgatcatggg cctttacgcg aactacccga
     6001 tcgcgctcgc gccgggcatg ggcctgaacg cctatttcgc gtacaccgtc gtgaaaggca
     6061 tgggtttcac gtggcaggcc gcgctcggcg cggtgttcat ctccggctgc ctgttcctga
     6121 tcgtcacgct gtttcgcgtg cgtgaggtca tcgtgaacgg cattccgcat tcgatacgca
     6181 tcgcgattac cggcggcatc ggcctgtttc tcgcgatcat ttcgctgaag tcggccggtg
     6241 tggtggttgg caatccggcc accctcgtca cgctcggcga tctccacaac ccgcatgcga
     6301 tcctcgcggt gatcggcttc tttgcgattg tcacgctcga ctatttgcga gtgcgcggtg
     6361 cgatcctgat cggcattgtc ggcgtgacgg tgctgagctt tttcttcggc ggcaatcagt
     6421 tccacggcat cgtctccgcg ccgccgtcga ttgcaccgac gctgttccag ctcgacattc
     6481 gcggcgcgct ctccggtggc gtgctgaacg tgatcctggt gttcttcctc gtcgaactgt
     6541 tcgatgcgac cggcacgctg atgggcgtgg ccaatcgcgc cgggctgctg gtcgaaggca
     6601 agatgcatcg gctgaaccgc gccctgctcg ccgacagcac ggcgattctg gccggctcga
     6661 tgctgggcac ctcgtcgacc acggcgtata tcgaaagcgc gtcgggcgtg caggccggcg
     6721 gccgcacggg tgtgacggcg atcaccgtcg ccatcctgtt cctcgcggcg ttgtttttcg
     6781 cgccgctggc gggcgtggtg cccggctacg cgacggcgcc ggccctgctg tatgtgtcgt
     6841 gcctgatgct gcgcgaaatg ctcgacctgc cgtgggacga cgccactgaa gtcgtgccgg
     6901 ccgccctgac cgccctgctg atgccgttca cctactcgat cgccaatggc gtcgcattcg
     6961 gtttcatctc gtatgccggc ctgaaactgc tgaccgggca ggcgcgcaag gtgaaactgg
     7021 tggtgtggat catcgcggcg attttcctgt tccggtattt ctacctgggc agcgaataag
     7081 cgcgcggctc cttcctgcac cgtgcggcgc cgtgcaacgc ggcgcggcct gccgtaaagc
     7141 atctcggcat tgacactgtg tctggttgtg gttcggtccg gatcaacaat cgttttattg
     7201 aaggcgcccg ttctggtcgg gca
//
LOCUS       DATASH010000347         7985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_445140,
            whole genome shotgun sequence.
ACCESSION   DATASH010000347 DATASH010000000
VERSION     DATASH010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7985
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445140"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..2162)
                     /gene="shc"
                     /locus_tag="VHV99_09695"
     CDS             complement(105..2162)
                     /gene="shc"
                     /locus_tag="VHV99_09695"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HEX3635745.1"
                     /translation="MNDLSQAQPLDAVLPEFADAALNASNPALNVSNTAPIAAPQASA
                     GPLDAAITRATDAILAAQNPDGHWVYELEADATIPAEYVLLVHYLGETPNVELEQKIA
                     RYLRRIQLAGGGWPLFTDGAMDVSASVKAYFALKMIGDSPDAEHMVRAREAILADGGA
                     ATVNVFTRILLALFGVVSWRAVPMMPVEIMLLPQWFPFHLSKVSYWARTVIVPLLVLN
                     AKRPVARNPRRVRIDELFRAAPVNTGMRDRAPHQHLGWFRFFRGVDVVLRTVDGLFPK
                     ATRERSVREAVKFVDERLNGDDGLGAIFPAMANAVMMYDVLGYPADHPKRAIARQSID
                     KLLVIKDDEAYCQPCLSPVWDTSLVAHALLETGEAHAEQAAERGLAWLRPLQILDVRG
                     DWISRRANVRPGGWAFQYNNAHYPDVDDTAVVVMAMQRSAALTQSDVDREAIARAREW
                     VIGMQSSDGGWGAFEPENTQYYLNNIPFSDHGALLDPPTADVSGRCLSMLAQLGEFPQ
                     GSEPAQRAFDYMLKEQESDGSWYGRWGLNYIYGTWTALCSLNAAGLPHDDPRMRRAAQ
                     WLLSIQNEDGGWGEGGESYKLDYRGYERAPSTASQTAWALMGLMAAGEVNHESVARGV
                     EYLQREQRGHGLWDETRFTATGFPRVFYLRYHGYRKFFPLWALARFRHLKRNGLTRVA
                     VGM"
     gene            complement(2159..3424)
                     /gene="hpnE"
                     /locus_tag="VHV99_09700"
     CDS             complement(2159..3424)
                     /gene="hpnE"
                     /locus_tag="VHV99_09700"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HEX3635746.1"
                     /translation="MQKLVHVIGAGLAGLAAAVQLQRRGAQVVLHDAAAQAGGRCRSY
                     YDPKLGVTIDSGNHTVLSGNFATLNYLRAIGAADELVGPAQPEYPFVDLATRARWTVR
                     MSPGRLPWWIFDPNARVPNTGPTDYLALVPLLFAKPGRSVAQTMRCNGPLWDRLLRPL
                     FLAMLNIEPREASAELTGAMVRETLVAGGLACRPLVARNGLSSAFVDPALRLLQHGGA
                     AIRLGSRLTDIVFADTGRVSALNFADESIAIDANQAVVLAVPPDSAQTLVPGLRSPSR
                     FAASANVHFAIEPPFGMAPLTGLLNATADWLFAFEGRVSVTINAAERLLDTPHEALAK
                     TIWADVARAVNLPAEPMPAWQVVMEKRATFAALPDQETLRPGTRTRWNNLMLAGDWTA
                     TGLPATIEGAIRSGQKAADTLLNQPMERR"
     gene            complement(3429..4277)
                     /gene="hpnD"
                     /locus_tag="VHV99_09705"
     CDS             complement(3429..4277)
                     /gene="hpnD"
                     /locus_tag="VHV99_09705"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006404656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HEX3635747.1"
                     /translation="MAVSNLVVDDTEIDAAAATSGSSFYLAMRILPAAQRDAMYQVYA
                     FCRAVDDIADSDLPRAERAAALERWRADIDACYAGAPRASLRALTRHIHTFHLQREDF
                     HAMIDGMAMDAAADICAPDEATLDLYCDRVASAAGRLSVRIFGMQEEPGRLLAHHLGR
                     ALQLTNILRDIDEDAGINRCYLPHELLAREGIAVTSPSQIADDPSLPRVCATLAERAK
                     QHFAASDAIMDREPRNHVRAPRIMSGVYRVLLERTLERGFDIPRTKVSKPKLRMLWIV
                     ARYALF"
     gene            complement(4460..5410)
                     /locus_tag="VHV99_09710"
     CDS             complement(4460..5410)
                     /locus_tag="VHV99_09710"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004555242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX3635748.1"
                     /translation="MNVSLDVPVKSLSDLVVERVGFGIFVLDRDMNVLMWNRFMQDHS
                     GLSPEQVVGKSLFTHFPEVPRVWLSRKIESVFQLGSFAFSSWEQRPYLFKFDHDRPIT
                     GGVDFMQQDCTFMPLMRDREVVAVCVTISDVTHVSIVQREREEAVAKLQEYADRDGLT
                     GIANRRFFEARLRDEYTRWQRYGGEMSVLLFDLDHFKKINDQFGHGVGDTVLRVMAQR
                     VADVVRAQDTFGRFGGEEFALLLPCTPLEDAMRVAEKIRCTIADTPVEVQGTSVPVTA
                     SVGGAAARVGVAAYDVLINEADAALYSAKRQGRNRSVAFI"
     gene            complement(5407..6030)
                     /locus_tag="VHV99_09715"
     CDS             complement(5407..6030)
                     /locus_tag="VHV99_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HEX3635749.1"
                     /translation="MSESVLTEDQRDALQEVANLAMGQAATRLASLLDAFIELSVPRV
                     KVVAVSEAARALREMTGIEDMVCAVRQGFRSDIKGEALVICRSDSIDQLCSLVSDPYS
                     RSTYEAVSQKELVFDVANVLTGACVSCILDQLGRTPVFSAPGLLGQALTLDEVFQPGV
                     LQWEVALLVEVNFALEDQSFRAHLVMLMAEESIRHMSEALDELLSSL"
     gene            complement(6023..6391)
                     /locus_tag="VHV99_09720"
     CDS             complement(6023..6391)
                     /locus_tag="VHV99_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004190980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3635750.1"
                     /translation="MPLPIVIADDSLLARKVLTKALPPDWDVDVSYATNGREALGLYR
                     KGKASVIFLDLTMPDMTGYQVLEALQHEDLNTFVIVVSADVQPMAQSRVRALGAAAFI
                     GKPVTPEAVLPILKEYGLYV"
     gene            7095..>7985
                     /locus_tag="VHV99_09725"
     CDS             7095..>7985
                     /locus_tag="VHV99_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEX3635751.1"
                     /translation="MTPDRFDPPEASRPPGSRQDAALFPAVPEQNFHVRRITLIALLI
                     AAIVLPCVYVAAMAFSDLRARMADATDVTLRTVRVAEEHALKVFDMNETLNARIVDLT
                     QGLDDDGIRAEEGGIHEKLRTMGGGYPQVAAVSIFGRQGDLLATSRFYPVPVLSIADR
                     EDFVGIRDGKDLEHVSKVMTGPVVGEQVFNTGVERRAADGSFAGVVSVALRPSYFDAF
                     YRELLGGGESTPMTMSLLRTDGAVLAHYPRRPREPVAVVPGSPLAEALAQGRRAGVVR
                     MHSELDGENVILAFRRVGAYP"
BASE COUNT         1490 a         2599 c         2529 g         1367 t
ORIGIN      
        1 ttgccgcggg cgatgcgcgc ttcgaacgcc atacccgtca ccacgatcac cggcaaggtg
       61 ccgcggccgg cgttagctga cggtgttgtc gaaaacgcca tccgttacat cccgaccgcg
      121 acgcgcgtca gcccgttgcg cttcagatga cggaagcgcg ccagcgccca caacgggaag
      181 aacttgcgat aaccgtgata acgcagatag aacacgcgcg ggaaaccggt cgccgtgaag
      241 cgcgtctcgt cccacaggcc gtgcccgcgt tgttcgcgct gcaaatactc gacgccgcgc
      301 gcaaccgact cgtgattgac ctcgcccgcc gccatcaggc ccatcagcgc ccacgcggtt
      361 tgcgaagccg tgcttggcgc acgctcgtag ccgcgatagt cgagcttgta gctctcgccg
      421 ccctcgcccc agccgccgtc ttcgttctgg atcgacagca gccactgcgc cgcgcgcctc
      481 atgcgcgggt cgtcgtgcgg cagaccagcg gcgttcagcg aacacagcgc ggtccacgtg
      541 ccgtagatgt agttcaggcc ccagcggccg taccagctgc cgtccgattc ctgttccttc
      601 aacatgtagt cgaacgcacg ctgcgccggt tcgctgccct gcgggaattc gccgagttgc
      661 gcgagcatcg acaggcaacg gcccgacacg tccgcggtcg gcggatcgag caacgcgccg
      721 tgatcggaga acggaatgtt gttgaggtag tactgcgtgt tttccggttc gaacgcgccc
      781 cagccgccgt cgctgctttg catgccgatc acccattcgc gcgcccgcgc aatggcttcg
      841 cgatcgacat ccgattgcgt cagcgccgcc gagcgttgca tcgccatcac gaccacggcc
      901 gtatcgtcga cgtccggata gtgcgcgttg ttgtactgga acgcccagcc gcccggacgc
      961 acgttcgcac ggcgtgagat ccagtcgccg cgcacgtcga gaatctgcag cggacgcagc
     1021 cacgcgagcc cgcgttcagc cgcttgctcg gcatgcgctt cgccggtctc gagcagcgcg
     1081 tgcgccacca gtgaggtatc ccacacgggc gacaaacacg gttggcaata ggcttcgtca
     1141 tccttgatga cgagcagctt gtcgatcgac tgacgggcaa tcgcgcgttt cggatgatcg
     1201 gccggatagc cgagcacgtc gtacatcatc accgcgttgg ccatggcggg gaaaatcgcg
     1261 ccgaggccgt cgtcgccatt cagccgttcg tcgacgaact tcaccgcctc gcgcaccgag
     1321 cgttcgcgcg tcgccttcgg aaacaggccg tcgaccgtgc gcaacacgac gtcgacgcca
     1381 cggaaaaagc ggaaccagcc gaggtgctga tgcggcgccc gatcacgcat gccggtgttg
     1441 accggcgcgg cgcggaacaa ttcgtcgata cgcacgcgac ggggattgcg cgccaccggc
     1501 cgcttcgcgt tcaacaccag caacggcacg atcaccgtgc gcgcccaata cgacaccttc
     1561 gacagatgga acgggaacca ttggggcaac agcatgattt ccacgggcat catcggcacg
     1621 gcgcgccatg acaccacgcc gaacagcgcc agcagaatcc gcgtgaacac gttcaccgtt
     1681 gccgcgccgc cgtccgccag aattgcctcg cgcgcgcgga ccatgtgctc ggcatccggc
     1741 gagtcgccga tcattttcag tgcgaagtac gccttcacgc tggcgctgac gtccatcgcg
     1801 ccgtcggtga agagcggcca gccaccgccg gcgagctgga tgcggcgcag atagcgggcg
     1861 atcttctgct ccagttccac gttcggcgtt tcgccgagat agtggaccag cagtacgtat
     1921 tcagcgggaa tcgtcgcgtc ggcttcaagt tcgtagaccc agtggccgtc cggattctgc
     1981 gcggcgagga tcgcgtcggt ggcacgcgtg atggcggcat cgagcggccc agcggaagct
     2041 tgcggcgcgg cgatgggcgc agtgttcgac acattcagcg cagggttcga cgcattcagc
     2101 gcggcatcgg cgaattcagg caggacggcg tccagcggct gggcttgaga taagtcattc
     2161 atcggcgttc catcggttga ttcagcagcg tgtccgcggc cttctggccg gagcggatcg
     2221 cgccttcaat cgtcgcgggc aggccggtgg ccgtccagtc gcccgcgagc atcaaattgt
     2281 tccagcgtgt gcgcgtgccg ggacgcagcg tttcctggtc cggcagggcg gcaaacgtcg
     2341 cgcgcttttc catcacgact tgccaggccg gcattggctc ggccggcaaa ttgacggctc
     2401 gggccacgtc ggcccaaatg gttttcgcga gcgcttcatg cggtgtgtcg agcaggcgtt
     2461 cggcggcgtt gatcgtgacc gacacgcggc cctcgaaggc gaacaaccaa tcggccgtcg
     2521 cattgagcag ccccgtgagc ggcgccatcc cgaacggagg ctcgatcgcg aaatgcacgt
     2581 ttgcgctcgc cgcgaagcgg ctcggcgagc gcaaaccggg cacgagcgtc tgtgcactgt
     2641 ccggcggcac cgcgagcacc acggcctggt tggcatcgat cgcgatgctt tcgtccgcga
     2701 aattcagcgc cgagacacga ccggtgtcag caaaaacaat gtccgtcagg cgcgagccaa
     2761 gccggattgc cgcgccgcca tgctgcaata gccgcagcgc cggatcgaca aaggcgctgc
     2821 tgaggccgtt gcgcgccacc agaggccggc acgccagacc gccggcgacc agcgtttccc
     2881 tgaccattgc gcccgtgagt tccgccgacg cttcacgcgg ctcgatatta agcatcgcga
     2941 gaaacagcgg ccgcagcagg cggtcccata gcgggccatt gcagcgcatg gtctgcgcca
     3001 cactgcgccc gggcttggcg aacagcagcg gcacgagcgc cagatagtcg gtcggcccgg
     3061 tgttcggcac gcgcgcgttc gggtcgaaga tccaccaggg caagcggccc ggcgacatgc
     3121 gcacggtcca gcgcgcccgc gtcgccagat ccacgaacgg atactcgggc tgggccgggc
     3181 cgaccagctc gtcggcggcg ccgatcgcac gcaaatagtt gagcgtggcg aaattgccgg
     3241 acagcaccgt atgattgccg ctgtcgatgg tcacgccgag cttcgggtcg taatacgaac
     3301 ggcaacggcc accggcctgc gcggccgcgt catgcaacac gacttgtgcg ccgcgccgtt
     3361 gcaattgcac cgcggcggcg agaccggcga gaccggcgcc gatcacatgg acgagctttt
     3421 gcatcagctc agaagagcgc gtaacgcgcg acgatccaca gcatgcgcag tttcggcttg
     3481 ctgaccttcg tacgcggaat gtcgaaaccg cgctcgagcg tgcgttcgag cagcacgcga
     3541 tacacgcccg acatgatgcg cggcgcacgc acgtggttgc gcggctcgcg gtccatgatc
     3601 gcgtcggagg cggcgaaatg ctgcttggcg cgctcggcga gcgtggcgca gacgcgcggc
     3661 aaagacggat cgtcagcgat ctgcgacggg ctcgtcaccg caatgccttc gcgcgccagc
     3721 aactcgtgcg gcaggtagca gcgattgatg ccggcgtctt cgtcgatatc gcgcaggatg
     3781 ttggtcagtt gcagcgcgcg gcccagatgg tgcgcgagca aacggcccgg ctcctcctgc
     3841 atcccgaata tcctcaccga caggcggccc gccgcactcg cgacacggtc gcaatacagg
     3901 tcgagcgtgg cttcgtcggg ggcgcagatg tcggcggcgg cgtccatcgc catgccgtcg
     3961 atcatcgcgt ggaagtcttc gcgttgcaga tggaacgtat gaatgtgccg cgtcagcgcg
     4021 cgcaaggaag cgcgcggtgc gccggcgtag cacgcgtcga tgtcggcacg ccagcgctcg
     4081 agcgcggcgg cgcgttcggc gcgcggcagg tcgctgtcgg cgatatcgtc gacagcgcgg
     4141 cagaaagcgt agacctgata catcgcatcg cgctgcgcgg ccggcagaat gcgcatcgcg
     4201 agataaaacg agctgccgga cgtggcggca gcggcgtcga tttctgtatc gtccacgacg
     4261 agattggaaa cagccaagac gatctccgct ggaggcgccc gctgaaaggc gttcaagaag
     4321 ttttaatgcc tacccgcagg gcagacgggc cgcgcgcgcg gacacgcgcg aaacaccggc
     4381 agggatgccg gaaatggcga aaagtatagc aatctttgtg agacgccgca gtttgggggc
     4441 gaataggggg ccggaaggct cagataaacg ccaccgagcg gttgcgtccc tgccgcttgg
     4501 cgctatacag cgccgcgtcg gcttcattga tcagcacgtc gtacgccgcc acgcccacgc
     4561 gcgccgccgc accgccgacg ctcgcggtca ccggcacact cgtgccctgc acttcgacag
     4621 gcgtatcggc gatcgtgcag cggatctttt ccgcgacccg catcgcgtct tcgagcggcg
     4681 tacagggcag cagcaaggca aactcctcgc caccgaaacg cccgaaggta tcctgcgccc
     4741 gcaccacgtc ggcgacccgc tgcgccatca cgcgcaacac cgtatcgccg acgccatgac
     4801 cgaactggtc gttgattttc ttgaagtggt cgaggtcgaa cagcagcacg gacatctcgc
     4861 cgccgtaacg ctgccagcgc gtgtactcat cacgcaggcg cgcttcgaag aaacgtcgat
     4921 tggcgatgcc cgtcagacca tcacggtccg catattcctg cagcttggcg actgcctctt
     4981 cacgttctcg ctgcacgatg ctgacatgcg tcacgtcgga gatggtcacg cacacagcca
     5041 ccacttcacg atcgcgcatg agcggcatga acgtgcaatc ctgctgcatg aaatcgacgc
     5101 cgccggtaat cggccggtcg tgatcgaatt tgaagagata cgggcgctgc tcccacgaac
     5161 tgaacgcgaa actgccgagc tgaaacacgc tttcgatctt gcgcgacagc cacacgcgcg
     5221 gcacttccgg aaagtgcgtg aagagcgatt tgcccaccac ctgttccggc gacaagccgc
     5281 tgtgatcctg catgaagcgg ttccacatca gcacgttcat atcgcgatca agcacgaaga
     5341 taccgaaacc gacacgttcg acaaccagat cgctcaacga cttcacaggc acatccaaag
     5401 acacattcat aggctggaca acagctcgtc gagcgcctcg ctcatgtggc gaatcgactc
     5461 ttcggccatc agcatcacga ggtgcgcgcg gaagctctga tcttcgagcg cgaaattgac
     5521 ttccaccaac aacgcgactt cccattgcag cacgccgggc tggaacactt cgtcgagcgt
     5581 cagcgcttga ccgagcaggc ccggcgcgga gaagaccggc gtgcggccga gctggtcgag
     5641 aatgcaggag acacacgcgc ccgtcagcac gttcgcgacg tcgaacacca gttctttctg
     5701 gctcaccgcc tcgtaggtcg agcgcgagta cggatcgcta acaagcgaac ataactgatc
     5761 gatgctgtcg ctgcggcaga tcaccagcgc ttcgcccttg atgtcggagc gaaagccctg
     5821 gcgcaccgcg cagaccatgt cctcgatccc ggtcatctcg cgcagcgcgc gagccgcctc
     5881 actcaccgcc accaccttca cgcgcggcac cgacaattcg atgaacgcgt cgagcaggct
     5941 cgccagccgc gtcgcggcct ggcccatcgc cagattggcg acctcttgca gcgcgtcgcg
     6001 ctggtcttcg gtgaggactg actcagacat acaacccgta ctccttgagg atgggtagta
     6061 ccgcctcggg cgtcacgggc ttgccgatga aggcggcggc gccaagcgcg cgcacgcgcg
     6121 actgggccat cggctgaaca tcggcggaga cgacaatgac gaaggtgttc agatcctcat
     6181 gctgtaaagc ttccagcacc tgataaccgg tcatgtccgg catcgtcagg tcgaggaaaa
     6241 tcaccgacgc tttgcctttg cgatacagac cgagcgcttc acgcccgttc gtggcgtagg
     6301 acacgtcgac atcccagtcc ggcggcaatg ccttggtgag caccttacgg gcaagcaacg
     6361 aatcgtcggc gatcacaata ggcaaaggca tgggcaggtc ggtaagcgga attagctctg
     6421 gcgcgttaac ggcggctagg ggcggtttct ttagcgcgcg gtctgtcgac ccgcggcaag
     6481 gcgatctgag gcctgcgaga atggagcttg caaacgagcg ggacgagcgg ggagtgcgcg
     6541 cggcgaggca cttttgacgg aaccgctttt gacagcaccc ctaggcacgc atggctgggt
     6601 gcgagcacgg tgaacgatgg ctatgaccgg catgttcgaa gcctaccccg tagatgaatc
     6661 cgcgcggctt catggatggg gcttggtccc cgtcgccgcg cgcgtgtttc cgcgagcgcc
     6721 ctccgcaacg cgttgtgcgc gtcgtcaggc tgccctcggc ggcagatgta tccgaacatg
     6781 gcggatacat attttatggt gaggtcgatt ttcgaagtaa aacggcggaa aggcaacccg
     6841 ccaaattcgc cccaactaaa aatagtttcc cactgaacgt tattttggaa aatattttga
     6901 tagattcagc gggtattctt ttggatttac acttttgtgt acgtttgcgc ttcttgattt
     6961 gtcactcatt agacggcaga caaatcagga gcgctcgttt gtcacggtaa cgagtagcgc
     7021 cgcgatgtgt ctatgctttt tgtaatgagg ccgacccccg gccctttcgt cccgaacgat
     7081 cctggttatc gaccatgacc ccagaccggt tcgacccgcc cgaagcgagc cgccctcccg
     7141 gcagccgtca ggacgcggcg ctgttcccgg ccgtgccgga acagaatttc cacgtccggc
     7201 gcatcacgct gatcgcactg ctgatcgcgg ccatcgtgct gccgtgcgtg tacgtcgcgg
     7261 caatggcgtt cagcgatctg cgcgcgcgca tggccgatgc gaccgacgtc acgctgcgca
     7321 ccgtgcgcgt agcggaagaa cacgcactga aagtgttcga catgaacgag acgctcaacg
     7381 cgcgcatcgt cgacctgaca caaggtctgg acgatgacgg cattcgcgcg gaagaaggcg
     7441 ggattcacga gaaactgcgc accatgggcg gcggctatcc tcaggtcgcg gccgtttcga
     7501 ttttcggccg ccaaggcgat ctgctcgcca ccagccgttt ctatcccgtg ccggtgttgt
     7561 cgattgccga tcgtgaagat ttcgtcggca ttcgcgacgg caaggacctc gaacatgtct
     7621 cgaaggtgat gacgggacct gtggtgggcg agcaggtgtt caacacgggc gtggagcggc
     7681 gcgccgccga cggctcgttt gccggcgtcg tctccgtcgc attgcggccc agctatttcg
     7741 acgcgttcta ccgggaactg ctcggcggcg gcgagagcac gccgatgacc atgagcctgc
     7801 tgcgcaccga cggcgcggtc ctcgcgcact atccccgccg cccgcgcgag ccggtggcgg
     7861 tggtgccggg ctcgccgctg gcggaggcac tggcgcaagg gcggcgagcc ggcgtcgtac
     7921 gaatgcattc cgagctcgac ggcgagaatg tgatcctcgc cttccggcgc gtcggtgcct
     7981 atccg
//
LOCUS       DATASH010000348         7490 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3809008,
            whole genome shotgun sequence.
ACCESSION   DATASH010000348 DATASH010000000
VERSION     DATASH010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7490
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3809008"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2893
                     /locus_tag="VHV99_09730"
     CDS             <1..2893
                     /locus_tag="VHV99_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010112934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX3635752.1"
                     /translation="LVDIQNRLKQVEPRLPQQVVQQGISVFKAANTFLMLVALTSTDG
                     HRDSVELSDYVNRYMLRDLKRAPGVGSAELWDAGEALRVWIDPMKLREYNLAAADVTQ
                     AIAAQNATVTAGTLGDVPFVTGQQLTASVTVRGQLISPGEFGEIVLKARPDGSAVRLK
                     DVARVELGRDSYASFSRLNGKSATTIGIRLSPQGNAMETSAAIRAKLAELSRSLPPGT
                     AVEIPFDNAKFVHVALREVALTLFEAVLLVFLVMWLFLRDLRYALVPTVVIPVALTGS
                     LLALYVLGMSINVFTMFAMVLAIGILVDDAIVVVENVDRVMREEGLAPRAATRRAMQQ
                     MGSAIVGITAVLTAVFVPMAFFPGSVGGIYRQFTVAMIASILVSAFTALSLTPALCAT
                     LLKAPPRSALRHGAHGSHEAGGRSFGFTAAFDWTSRKYRGLVARVLRRAGPMFAVYLV
                     LAGACALLYLHMPGGFLPQEDQSQLQVMVQLPAGATQARTLATVEQAETILKREPAVA
                     NVTSVLGWSFQGSGQNVAMCFVDLKDWSARDTDAATLRDTLNRSLSKIMDGTVAAQMP
                     PSVPGMGHSEGFVMRLEDRGGIGIAALSAAREQLFEQARHNPAFADVHSEALPDAPRI
                     VLDVDRAKAYALGVSFDKIGDALGNTFGSSYIDDFPSGGRMRRVMVAADASSRMTEDS
                     LMSLSVRNAAGQMVPLSSVASLHWTTGPSVLTRYNGSPSLDVSGRPANGTSSGAAMAE
                     MERLAQALPVGVGYDWTDSALEETRAARLTPILIGLSLLAVFMALAALYESWTIPLAV
                     LTIVPLGVIGAVIAMLLRGMPNDVYFKVGMITVIGLSAKNAILIVQFARTLYADGAPL
                     TDAIVAAASARFRPIVMTSAAFLLGVVPLLVSTGAGAESRRSIGTGVFGGAITATVLG
                     LVFTPLAFYIVVSGQRFIQSRMKSMRREKVTANVESEAPRRDVQGSQEG"
     gene            2995..3297
                     /locus_tag="VHV99_09735"
     CDS             2995..3297
                     /locus_tag="VHV99_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011547642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635753.1"
                     /translation="MQFPAGSVQALSSASSTVFSIGIVFLGYWGIHEGSHWRSGDISV
                     ALLALAGFACLGLVPWLATSPTKRADDESRLRLSRHAFLGGVGALWLAIVVSVFTQ"
     gene            3359..3997
                     /locus_tag="VHV99_09740"
     CDS             3359..3997
                     /locus_tag="VHV99_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011853312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VC0807 family protein"
                     /protein_id="HEX3635754.1"
                     /translation="MKIRPGLVVELGVNLVLPWLAYRLALPHWGELGALYASAVPPVI
                     WSLVEFARTRRVDALSILVLLGIALSVVLMALGGSPRLLLMRESMASGAIGVVFLLSL
                     AMRRPLTFYLARATVAREGEGGAERFEALWNERPALRRSVRLMTAVWGLGLTGENALR
                     SWLAWHWPIERFLVVSPFIGYGIYGALTVWTLVYRKKLQRRATAAPTGAVGP"
     gene            complement(4210..5928)
                     /gene="msbA"
                     /locus_tag="VHV99_09745"
     CDS             complement(4210..5928)
                     /gene="msbA"
                     /locus_tag="VHV99_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014896445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HEX3635755.1"
                     /translation="MSRLWPYIRPLIWIVALGLIAMGFVAASEAGIPMLLKPLLDHGF
                     GAKGSASAKWIVPAAVVGLAVVRGGAQYASGYLLAYVTNKILLELRLKMFDRMIHTSA
                     GFFQRETASTIINAIVFEVNQILNVLQSVVVTLVRDSLTVIFLLGYLFILNWRLTLVI
                     AVILPAIGWLVSKINRRLRRLNRESQLLTNELSYIVEETVGGYKVVKVHNGQRYENDR
                     FTSMSNRLRGYQMRMTVSGGLAQPLTQFLASIALAIVITIAVVQSANDQTTVGGFVAF
                     VTSMLLVISPLKHLIDINQPLQRGMTAAELIFGLIDEPAEPEGGGRPLVRASGEVEFR
                     EVSFSYGSTGTPTLDAISFKIAPGEMVALAGASGSGKTTLVNLLPRFFDPASGAVLVD
                     GVPLQEYNLHDLRGQMAMVSQDVVLFNDTIAANVAYGQTPDRERVMAALAAANLADVV
                     AAMPEGVDTRVGGNGMRLSGGQRQRLAIARAIYKDAPILILDEATSALDSESERHVQE
                     ALETLMKGRTTLVIAHRLSTIERADRILVMDAGKIAEQGSHAELLRHNGLYANLHRIQ
                     YQQQAA"
     gene            complement(6201..7478)
                     /locus_tag="VHV99_09750"
     CDS             complement(6201..7478)
                     /locus_tag="VHV99_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010114476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX3635756.1"
                     /translation="MQVERVLWVACPVIMFVAMFAHMTALENMALGLIGIGTLAAACS
                     SERPSPLHWPLLWPIVIWSAWALASIAWSPYPRDSMHAWLDEVLYPLVSFFAFWLIGT
                     RTTKPEWMVSINWVACVLLAAGSLYYWGRLQPPTNPSDILLHYYNRVGHTSTLAIFSM
                     TLFTGLLLNRRWWPIALSGLVLGLVIGLATLNRFFWPAAAVTLVVACYPLYRKHLVIA
                     GVCVAVICVAAVGTLELSARMRYGHAMPKAAARELSVGGDKVVVPEALAGIGDVMSSD
                     TRPKLWAFYGREGEAHTWTGIGFGKPLPGRVFQNEIPPALLETEPQALTHAHNLFLNT
                     WLETGIVGVVLEAALLIALIWRFWRLRHVVPWLSSAGMALVIGMIAKNFTDDFMWQTT
                     ALAFWCFSGFLLGRGERLAGRVAPARGALSQQQ"
BASE COUNT         1309 a         2386 c         2371 g         1424 t
ORIGIN      
        1 gctggtcgac atccagaacc ggctcaagca ggtcgagccg cgtttgccgc agcaggtcgt
       61 gcagcagggc atcagcgtat tcaaggcggc caacacgttc ctgatgctgg tcgcgctcac
      121 gtccaccgac ggccaccgcg attccgtgga actgagcgac tacgtcaacc gctacatgct
      181 gcgcgacctg aagcgcgcgc cgggcgttgg ctcagccgaa ttgtgggacg ccggagaggc
      241 gttacgggtc tggattgatc cgatgaaact gcgcgaatac aacctggccg ctgccgatgt
      301 cacccaggcc atcgcggcgc aaaacgcgac agtcacggcc ggcacgctcg gcgacgtgcc
      361 cttcgtcacc ggtcagcaat tgaccgcgtc ggtcacggtc agggggcagc tgatctcgcc
      421 gggcgaattc ggcgagatcg tgctgaaagc gcgtcccgat ggatcggccg tccggctgaa
      481 ggatgtcgcg cgggtggaac tgggccgcga cagctacgcg tcgttttcgc gtctgaacgg
      541 caagtcggcg acaacgatcg gtatccggct ctcgccccaa ggcaacgcga tggaaacctc
      601 cgccgcgatc cgcgcgaagc tggcggaatt atcgcgtagc ctgccaccgg gcacggccgt
      661 ggaaattccg ttcgacaacg ccaagttcgt ccacgtcgca ctgcgcgaag tggcgctgac
      721 gctgttcgaa gccgtgctgc tcgtgttcct ggtcatgtgg ctattcctgc gcgacttgcg
      781 ttatgcactg gtgcccacgg tggtgattcc ggttgcgttg accggctcgc tgcttgcgct
      841 ttatgtactc ggcatgtcga tcaacgtgtt caccatgttc gcgatggtgc tggcgatcgg
      901 gatcctggtc gacgatgcca tcgtcgtggt cgagaacgtc gatcgcgtga tgcgcgaaga
      961 ggggttggcg cctcgcgcgg ccacgcgccg ggcgatgcag cagatgggca gcgcgatcgt
     1021 cggcattacc gccgtgctca ccgccgtgtt cgtaccgatg gcgttcttcc ccggcagcgt
     1081 cggcggtatt taccggcagt tcacggtcgc gatgatcgcg tcgattctcg tgtccgcgtt
     1141 cactgccctt tcactgacgc cggcgttgtg cgcaaccctg ctgaaagcac cgccacggag
     1201 tgccttgcgg cacggtgctc acggttcgca cgaggccggc ggccgttcgt tcggcttcac
     1261 ggcagccttc gactggacct cgcggaaata tcgcgggctc gttgcacgcg tgctgcgccg
     1321 cgcgggtccg atgttcgcgg tgtatctcgt gctggccggc gcgtgcgcgt tgctttatct
     1381 gcatatgccg ggcggcttct tgcctcagga ggaccagtcg cagcttcagg tgatggtgca
     1441 gttgccggcg ggcgccaccc aggcgcgtac gctcgcgacc gtcgagcagg cggaaacaat
     1501 cctgaaacgc gagccggcgg tggccaacgt gacaagcgtg ctcggctgga gtttccaggg
     1561 cagcggccag aacgtggcca tgtgttttgt cgatttgaag gactggtcgg cgcgcgatac
     1621 cgacgcggcc acgctgcgcg acacgctgaa tcgctcgctc tcgaagatca tggacggcac
     1681 cgtggctgcc cagatgccgc cgtcggttcc gggcatgggc cattcggaag ggtttgtgat
     1741 gcggctggaa gatcgcggcg gcattggcat tgccgcgctg agcgccgcgc gcgaacaact
     1801 cttcgagcag gcccgccaca atcccgcttt cgccgatgtg cattcggaag cgttgcccga
     1861 cgcaccccgc atcgtgctgg acgtcgaccg cgcgaaggct tatgcgctgg gcgtttcgtt
     1921 cgacaagatc ggcgacgcgc tggggaacac cttcggctcg tcgtatatcg acgacttccc
     1981 ttcgggcggc cgcatgcgcc gcgtgatggt cgccgccgac gcctcttcgc gcatgaccga
     2041 agacagtctg atgagcctct cggtgcgtaa cgcagcgggg cagatggtgc cgttgtcctc
     2101 cgtggcgtcg ttgcactgga cgaccgggcc gtcagttctt acgcgctaca acggttcgcc
     2161 ttcactcgat gtcagcggcc gtcccgccaa cggcacgagt tccggcgcgg cgatggccga
     2221 gatggagcgg ctcgcccaag cgctgccggt gggcgtgggt tacgactgga ccgactcggc
     2281 gcttgaagag acgcgtgccg cccgcctgac gccgatactg attggcttgt cgctactggc
     2341 cgtgttcatg gcgctcgcgg cgctttacga gagctggacc atcccgctcg cggtgctgac
     2401 tatcgtgccg ctgggcgtga ttggcgcagt gatcgcgatg cttctgcgcg gcatgccgaa
     2461 cgatgtctat ttcaaggtcg ggatgatcac ggtgattggg ttatcggcga aaaacgcgat
     2521 cctgatcgtg cagttcgcgc gcacgctgta cgccgatggc gcgccgttga cggacgccat
     2581 tgtcgccgcg gcgagtgcgc gcttccggcc gatcgtgatg acatcggcgg cattcttgct
     2641 gggcgtcgtg cctttgctgg tgtccactgg cgccggggca gaaagccggc gatcgatcgg
     2701 gaccggcgta ttcggcggtg ccattacggc gaccgtactg gggctggtgt tcacgccgct
     2761 ggcgttttat atcgttgtat cggggcagcg gtttattcaa agccggatga agtccatgag
     2821 acgggagaag gtcacggcga acgtcgagtc cgaagcgccg cggcgcgacg ttcaaggctc
     2881 gcaagaagga tagcgtccag gcgcggcgct ctactgtcag cctccccgtt ccaccgatgc
     2941 gattccccta tgatggcgac atctcgcgta ctttcgcgtg tcgggaggca tgtcatgcag
     3001 tttccagcag gatccgttca ggcgctcagt tcagcctcat cgacggtgtt ttccatcggc
     3061 attgtgttcc tcggttactg gggtattcac gagggctcgc actggcgctc cggcgatatc
     3121 tccgtcgccc tcctggcgct cgccggtttc gcgtgcctgg gcctggttcc ctggctggcc
     3181 acgtcgccaa caaaacgcgc cgacgatgaa tcccgcctgc gtctctcccg tcacgcgttt
     3241 ctgggtggcg tgggtgcgtt gtggctggcg atcgtcgtgt ccgttttcac gcaatgacgg
     3301 tcgttcgcgc gatggtttta gtcgtcggcg cccttccctt cctcagcaca agttcctcat
     3361 gaaaatacga cccggtcttg tcgttgaact cggggtcaat ctcgtgctgc cgtggcttgc
     3421 gtatcggctc gcattgccgc attggggcga actcggcgcg ctgtacgcgt cggcggtgcc
     3481 gcctgtcata tggagtctcg ttgagttcgc aagaacccgt cgcgtcgatg ccctcagcat
     3541 cctggtcttg ctcgggatcg cgctgtccgt cgtgctgatg gcgctcggcg gcagtccgcg
     3601 gttattgttg atgcgcgagt cgatggcctc gggggcgatc ggcgtagtgt ttctgctatc
     3661 gcttgcgatg cggcgtccgc ttacctttta tctcgctcgg gcaaccgtcg cacgtgaagg
     3721 cgaaggcggc gcggaacgct tcgaagcgct ctggaacgaa cggcccgcgc tgcgtcgatc
     3781 ggtgcgtctg atgacggccg tgtggggact cggcctgacc ggcgagaatg cgcttcgaag
     3841 ctggctggca tggcattggc cgatcgagcg tttcctggtc gtctcgccgt tcatcggtta
     3901 tggcatctat ggcgcgctga cggtatggac gctggtgtat cgcaagaagc tgcagcggcg
     3961 tgctacggcc gcgcccacag gagctgttgg tccatagcgc tttggggcac ctcggcccgc
     4021 agggcagcat caatgtggcg atgagttcac aagcccgaag cccgcgtttc gtcgcgttgc
     4081 cttccacgcg aggctccgct gaacaccatc cgccacgcag gacacagccg gccttttcac
     4141 gcgccccaac gcaaaacgcg ccgccagccg ttccggccag gggcgcgcat cgattaaaac
     4201 gaaaaagctt caggctgcct gctgctgata ctggatccga tgaaggttcg catacagccc
     4261 gttgtggcgc agcaactcgg cgtggctgcc ctgttcggca attttcccgg cgtccatcac
     4321 cagaatccgg tcggcacgtt caatggtcga caagcggtga gcaatcacca gcgtcgtgcg
     4381 ccccttcatc agcgtttcca gggcttcctg cacatgccgt tcggactcgg aatcgagcgc
     4441 ggacgtcgct tcgtcgagaa tcagaatagg cgcgtccttg tagatcgccc gagcaatagc
     4501 cagccgctgg cgctgcccgc ccgacaagcg catgccgttg ccgcccaccc gcgtgtccac
     4561 accttcgggc atggccgcaa cgacatcggc gagattcgct gcagccagcg ccgccatcac
     4621 ccgctcgcga tccggcgtct ggccgtatgc gacgttggcg gcgatcgtgt cgttgaagag
     4681 caccacgtcc tggctcacca tcgccatttg tccgcgcaga tcgtgcaggt tgtactcctg
     4741 aagcggcacg ccgtcgacca gcaccgcacc gctggcagga tcgaagaaac gcggcaacag
     4801 gttgacgagc gtggtcttgc cgcttcccga tgctccggcc agcgccacca tttctcccgg
     4861 ggcgatcttg aacgaaatcg cgtcgagcgt cggcgtcccg gtcgatccgt aactgaacga
     4921 cacttcgcgg aactccacct cgccgctggc gcgcacgagc ggccggccgc caccttcggg
     4981 ctcggccggt tcatcgataa gaccgaagat cagttccgcc gccgtcatgc cccgctgcag
     5041 cggctggttg atatcgatca ggtgcttgag cggcgagata accagcagca tggaggtgac
     5101 gaacgccacg aatccgccga cggtggtctg gtcgttcgcc gactgcacca cggcgatggt
     5161 gattacgatg gcgagtgcaa tcgatgccaa aaactgcgtg agcggctgcg cgagaccgcc
     5221 ggacaccgtc atgcgcatct ggtagccgcg caggcgattg ctcatgctcg tgaaccggtc
     5281 gttttcgtag cgctggccgt tatggacctt gacgacctta taaccgccca ccgtctcttc
     5341 cacgatgtac gacagttcat tcgtcagcaa ctgcgattcg cgattgaggc gccgcaaccg
     5401 tcgattgatc ttgctcacca gccagccgat agccggcagg atcaccgcga tgaccaacgt
     5461 cagccgccag ttgagaatga acaggtatcc cagcaggaat atcactgtga gcgaatcacg
     5521 cacaagcgtg acgaccacgc tctggagcac gttgaggatc tggttcacct cgaacacgat
     5581 ggcgttgatg atcgtgctgg ccgtttcccg ctggaagaaa ccggcgctgg tgtggatcat
     5641 ccggtcgaac atcttcaggc gcagttcgag caggatcttg ttggtgacat aggcaagcaa
     5701 gtagccagac gcgtattgcg cgccacctcg cacgaccgcg aggccgacga ccgcggccgg
     5761 cacgatccac ttggcgctgg cgctgccctt cgcgccgaag ccgtgatcaa gcagcggctt
     5821 gagcagcatg ggaatgcctg cctcgcttgc ggcgacgaaa cccatggcaa taaggccgag
     5881 cgcaacgatc cagatcaggg ggcggatgta cggccagagc cggctcatca cggcggccgg
     5941 cgacgtggtc tccccgctgc cgatcgtttt gctcagggtt ggcttttgac tcaaaagatg
     6001 tccttaatcg tggatccgtg aatccgtggg tcgggggcga gcggagcagg actgaacctg
     6061 cgcccgggcg actctgggcg cgctgaaccg cgccgacacg gcaaagcggt attgtaatgc
     6121 gccggggccc tttgtctcat taagaggctt gtccgggaga atgccgcagc aaggtcgggt
     6181 tgcggccttt agtgcggcct ctactgctgc tgcgacaaag cccctcgtgc cggcgcaacc
     6241 cgccccgcca gacgctcacc acgaccgagc aggaaccctg aaaaacacca gaacgcgagc
     6301 gcggtggtct gccacatgaa gtcgtcggtg aaattctttg caatcattcc gatgaccagc
     6361 gccatgcccg ccgaactcag ccagggcacc acatgccgca gccgccagaa acgccagatc
     6421 aacgcaatca gcagcgccgc ctccagcacc acgcccacta ttcccgtctc cagccacgta
     6481 ttcaaaaaca gattgtgcgc atgtgtcagc gcctgcggtt ccgtctccag caatgcaggc
     6541 ggtatttcgt tttgaaatac ccgccccggc aacggcttgc cgaaaccaat gcccgtccac
     6601 gtatgcgctt caccttcccg gccgtagaac gcccacagtt tgggacgcgt atcggaagac
     6661 atgacgtctc cgatgcccgc cagtgcctcg gggacaacca ccttgtcccc gcccaccgac
     6721 agctctcgtg cagcggcttt cggcatggca tggccgtaac gcatgcgcgc gctcagctcg
     6781 agcgtgccca ccgcggccac gcagatcacg gccacgcaca cgcccgcgat caccaggtgc
     6841 ttgcggtaca gcggatagca cgcgaccacg agcgtcacgg ccgcggcggg ccagaagaac
     6901 cggttgagcg tcgccagccc gatgacaagg cccagcacca gtccgctcag ggcgatcggc
     6961 caccagcggc gattcagcaa caggccggtg aagagcgtca tcgaaaagat ggcgagtgtg
     7021 ctcgtgtggc cgacgcggtt gtaatagtgg agaaggatgt cagagggatt cgtgggcggc
     7081 tgcaaacggc cccagtaata caggctgccg gcggccagca gcacgcaggc cacccagttg
     7141 atcgacacca tccactcggg cttcgtcgtg cgcgtgccga tcagccagaa cgcgaagaac
     7201 gacaccagcg gatacaacac ttcgtcgagc caggcgtgca tgctgtcacg cggatacggc
     7261 gaccacgcga tcgacgccag cgcccacgca ctccagataa caataggcca cagcaacggc
     7321 cagtgcagcg gcgacggccg ctccgaagaa caagccgcgg cgagtgtgcc aatacctatc
     7381 agtcccagcg ccatgttctc gagcgcggtc atatgggcga acatcgcgac aaacataatc
     7441 actggacacg ccacccacag aacgcgttcc acctgcatac acgtcttcgt
//
LOCUS       DATASH010000349         9678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_842208,
            whole genome shotgun sequence.
ACCESSION   DATASH010000349 DATASH010000000
VERSION     DATASH010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 9678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9678
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842208"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..1070
                     /locus_tag="VHV99_09755"
     CDS             129..1070
                     /locus_tag="VHV99_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX3635757.1"
                     /translation="MSPQLPSRFPRGLPRGFQIRLPQSRSGQIALALAVVYLVWGSTY
                     LAVHVALGSFPPLLLSGLRNLFAGVGLFIFAARRNPVWPGAGEIRNAGLVGTMLVGLS
                     SGMLAYGMRTVGTGTAAVMVATVPLFATVIAAVAGRKIGRGEWFAVGLGLVGIAILSH
                     GDSAPGSTGGSLAILCGALFWAGGAHLAGRLKLPSDLFLSTSLQIGLGGAMSTLVAWV
                     SGERMMAVHFLPVLAFIYLMLIGTMAAYVAYGFLIRHTSPIIASSCMYVNPVVAVALG
                     AVLLGEPVTDSTVIATVVILASVGLSFWFDYRKKGLA"
     gene            complement(1198..3297)
                     /locus_tag="VHV99_09760"
     CDS             complement(1198..3297)
                     /locus_tag="VHV99_09760"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008670 - 2,4-dienoyl-CoA reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0033543 - fatty acid beta-oxidation,
                     unsaturated, even number, reductase/isomerase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HEX3635758.1"
                     /translation="MPYQQLLAELDLGFTRLRNRVVMGSMHTGMEDRFWNYPKLAAYF
                     RERAKGGVGLIVTGGISPNRQRWLLPFGGTLNSVFDLRNHRLLTEAVHAEGGKIALQI
                     LHAGRYGYQPFVVSASALKSPISKFKPRALTEAGIARTVRDYARCARLAQRAGYDGIE
                     IMGSEGYLLNQFLCPRTNRRTDRYGGSIENRMRLAREIVERVRAVCGERFIVVYRISL
                     IDLVEGGNTWEETVQVAQALEAAGVTMFNTGIGWHEARVPTIVTSVPRAAFAALSARL
                     KAAVKVPVIVSNRVNTPEVAEELLAQGAGDLVSMARPLLADPEFVLKTAEGRAHEINT
                     CIACNQACLDHTFRNQRASCLVNPRAGRETELVYRPLAGPQAARSVAVVGAGPAGLSA
                     ASVAASRGHRVTLFDASATIGGQFNLAMRVPGKEEFSETIRYFQSQLQRHRVDVRLDT
                     RIDPALLAAGAYDDVIIATGIVPRRPAIAGIDGGNVLSYVEVLRGAPVGRRVAVIGAG
                     GIGFDVSEFLLHRSGDPLPMPRDAWLAEWGVDLAVRERGGLKPPAPVQPFREIWLLQR
                     KAGKLGMGLGKTSGWVHRATLARNGVNMLGGVSYREISERGLKIAREGVEEWLEVDTI
                     VVCAGQESLRDLYPETAARGADKTSGANGTQGANHATSPTGVKVPRYHVIGGAKLATE
                     LDAKRAIREGAEVAAAL"
     gene            3661..4455
                     /locus_tag="VHV99_09765"
     CDS             3661..4455
                     /locus_tag="VHV99_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HEX3635759.1"
                     /translation="MKMIRTMAAVVAVASVLTACGGGSDDVGKELGLTNPEIHFIHAI
                     PGGPAVDFLVNGKAPSGQTNISYKGVTNFTNINTGSTTVAYSATGTTTALASGNFPDV
                     AKGHEYTVLALPGLAAPDIGLIDDPFDKGLLSNSARLRGFNASANATNLDFYLVQATS
                     TDISKVSPTMAGVSFKNAVPASGQDSIYVSGGQYVLIATVAGSKTPIFQSQSFSLSNN
                     ADWLVTSVPIAGTLSQLVPGQIHLLIAQNGNTSTPSVELANALTGQ"
     gene            complement(4722..4856)
                     /locus_tag="VHV99_09770"
     CDS             complement(4722..4856)
                     /locus_tag="VHV99_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HEX3635760.1"
                     /translation="MNRLIALLLIAGTAALAGCNTMAGAGQDISKGGNAITNSAEQAK
                     "
     gene            complement(5088..6494)
                     /locus_tag="VHV99_09775"
     CDS             complement(5088..6494)
                     /locus_tag="VHV99_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX3635761.1"
                     /translation="MRNRSWAIAMTVAIVITVGGLVILETARERIAAEYETALEARDI
                     TVQLSTLASQLASLSAHQSDFLLTKRDASARGFCATAVRIRQNEQQLSSFYRGGRADS
                     AELASFTQIAALIDARISAGAAALQRAVPQTLDLSACGTASEANPADATLVDSTLTLL
                     RDNEERRAQHALDASRADQRISTLCAAGLSALNIVLFILLFRNLGIQMDRQARVQRQL
                     ITQQEELDQLVNERTRQLEALGWHLQAVSENEKTQLARELHDELGAILTASKMDVAWA
                     NRKLKDSAPDISEKLKRALANLDQGIALKRRIIEDMRPTVLANFGLVTALRTLADEAA
                     QRNGWTLESHLPADDIQLDEQTEIALFRVAQESLTNAAKYAHASRVSIALQVGDGEVA
                     LHIADNGVGITPADLKRTHTHGLLGMRQRVAAHGGRFDIQRGVPHGTDIHVVMPSVST
                     ATPSIDRPPSTPSRTSVE"
     gene            6799..6975
                     /locus_tag="VHV99_09780"
     CDS             6799..6975
                     /locus_tag="VHV99_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HEX3635762.1"
                     /translation="MLKWALFFAVVAVIAGLLGFTGVAAGAAAIAKFLFVVFVILCVV
                     FLVLGFVVTKKIID"
     gene            7117..7278
                     /locus_tag="VHV99_09785"
     CDS             7117..7278
                     /locus_tag="VHV99_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008350559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 family protein"
                     /protein_id="HEX3635763.1"
                     /translation="MLHYAIVFFVIAIIAAVFGFTGIAAGAAEIAKILFYIFLVVFVV
                     TLLLGVFRT"
     gene            7367..7939
                     /locus_tag="VHV99_09790"
     CDS             7367..7939
                     /locus_tag="VHV99_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX3635764.1"
                     /translation="MAKSSGAQGAASQSSDPFVMDIKKIREDARKHMSDGPVTQSYGA
                     DRETVLKLLNDSLATEIVCVLRYKRHHFMAKGINSEAVAAEFAEHAAEEQEHADRIAE
                     RIVQLGGEPDFAPDGLKTRSHSEYKEGDNLTDMIRENLIAERIAIDTYREIVHYLGEK
                     DVTTRRLFEEILAVEEEHADDMADLLEGRE"
     gene            8404..9036
                     /locus_tag="VHV99_09795"
     CDS             8404..9036
                     /locus_tag="VHV99_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3635765.1"
                     /translation="MIRVLIADDHAIVRGGFRQFVADEPDMCVAAEAATGDETIRLVR
                     EQAFDVVLLDIAMPDKNGIDTLRVIRQVRPEQGVLILSGYQESQYAINLLRAGANGYL
                     NKDCEPEEIVRAIRAVARGHRYLSEAVADTLADNLDKPAAGRPHEALSEREFQIFCKL
                     AAGQIPTEIAEELHLSVKTVSTYRARVLEKMRLANNADLTYYAIKNGLIE"
     gene            9057..9485
                     /locus_tag="VHV99_09800"
     CDS             9057..9485
                     /locus_tag="VHV99_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3635766.1"
                     /translation="MNSESNPVAEPASRAPLRVLLIEDSPLIRRSLVEAIDASGLLRV
                     AAYADSADQAIALLSDEAFDAVIVDLQLKQGSGVPVLAYLQREGMIDSIFAAVLTNHA
                     LPAYRERCEQYGVRHFYDKSFEFDRVIDALHEYAFARGAG"
BASE COUNT         1712 a         2863 c         3129 g         1974 t
ORIGIN      
        1 cttctgttcc accgacatgt ccgccagcct cgcccgccag accgcatcgc cgatgcggtg
       61 tccgccatcc cgaatgaggg cttttagcgg gggcgcagca cctcgagcag cacaggcgga
      121 ttgaacccat gtcgccgcaa ctcccaagcc gctttccgcg tggtttgccg cgtggtttcc
      181 agattcgttt gccgcagtcc cgtagtggac agatcgcgct cgcgctcgcc gtcgtctatc
      241 tcgtgtgggg ctcgacctat ctcgccgtgc acgtcgcgct cggttcgttt ccgcccttgc
      301 tgctctcggg tctgcgcaat ctgtttgccg gcgtagggct cttcatcttc gcggcgcgcc
      361 gcaatccggt ctggccgggc gcgggtgaaa tccgcaatgc cggccttgtc ggcacgatgc
      421 tggtcggctt gtcgagcggc atgctggcgt acggcatgcg caccgtcggc acgggcaccg
      481 cggccgtgat ggtcgcgact gtgccgctgt tcgcgacggt gatcgcggcg gtggcggggc
      541 gcaagatcgg cagaggcgag tggttcgcgg tgggcctcgg gctggtgggt atcgcgatcc
      601 tgagtcatgg cgatagtgcg ccggggtcga cgggcggcag tctcgcgatt ctctgcggtg
      661 cgctcttctg ggccggcggc gcgcatctgg ccggacggct gaaactgccg tcggacctgt
      721 tcctatcgac gtcgctgcag atcggcttgg gcggcgcgat gtcgacactg gtcgcgtggg
      781 tgtcgggcga gcggatgatg gcggtgcatt ttctgccggt gctggccttc atctacctga
      841 tgctgatcgg cacgatggcc gcctacgtcg cgtacggctt tctgatccgg catacgagtc
      901 cgatcatcgc cagcagttgc atgtatgtga atccggtggt cgcggtggcg ctcggggcgg
      961 tgttgcttgg tgagccggtc acggattcga cggtgattgc gaccgtggtg attctggcca
     1021 gcgtgggtct gtctttctgg ttcgattacc ggaagaaggg cctcgcgtga ggcgcgggcg
     1081 gctcgctgtc tgccgcctgc ctctcgctct tcggtcgccg cgttcggtgg ttccttgctc
     1141 gagagcatgg cggtcactta cgctagccgc cgcgtcacag cagagcggcc actttcgtca
     1201 gagcgccgcc gccacttccg cgccttcgcg aatcgcgcgt ttcgcatcca gttcggtcgc
     1261 gagcttcgcg ccgccgatca cgtgatagcg cggaaccttc acaccagtgg ggcttgtcgc
     1321 gtggttagcg ccttgcgtcc cattcgcacc actcgtcttg tccgcccccc gcgccgccgt
     1381 ctccggatac agatcgcgca gcgattcctg cccggcgcac acgacgatcg tgtccacctc
     1441 gagccattct tcgacacctt cgcgcgcaat cttcaaaccg cgttcgctaa tctcccgata
     1501 cgacacgcca cccagcatgt tcacgccgtt tctggccagc gtcgcgcgat gcacccagcc
     1561 cgaggtcttg ccgagcccca tgccgagctt gcccgccttg cgctgcaaca accagatctc
     1621 gcggaacggc tgcaccggcg ccggcggttt caagccgccg cgctcccgta ccgcgagatc
     1681 gacgccccat tcggcgagcc atgcatcgcg cggcatcggc agaggatcgc ccgagcgatg
     1741 caacaaaaac tcgctgacgt cgaaaccgat cccacccgca ccgatcaccg cgacgcggcg
     1801 tcccaccggc gcaccgcgca gcacctccac gtaagacagc acgttcccgc cgtcgatgcc
     1861 ggcaatcgcc gggcgccgtg gcacgatgcc ggtggcgatg atcacatcgt cgtaagcgcc
     1921 tgccgcgagc agggcaggat cgatgcgcgt atcgagccgc acgtcgacgc gatgccgctg
     1981 caactggctc tgaaaatagc ggatggtttc gctgaactct tccttgcccg gcacgcgcat
     2041 cgccagattg aactggccgc cgatggttgc gctcgcatcg aataaggtca cgcgatgccc
     2101 gcgcgatgcc gcgacgctcg ccgcggaaag tcccgccgga cccgcgccga ccaccgcgac
     2161 cgaacgcgcg gcttgcgggc ctgcgagcgg ccgatagacc agctcggtct cgcggcccgc
     2221 acgcggattc acgaggcaac tcgcgcgctg gttcctgaac gtgtgatcga ggcaggcctg
     2281 attgcaggcg atacaggtgt tgatttcgtg cgcgcgtccc tcggcggtct tcagcacgaa
     2341 ctcgggatcg gccagcagag gtcgcgccat cgacaccaga tcgcccgcgc cctgagcgag
     2401 cagttcttca gccacctcgg gcgtattgac gcggttcgac acgatcacgg gcaccttgac
     2461 cgccgccttc agccgcgcgc tgagcgcagc gaaagcagcg cgcggcaccg acgtcacgat
     2521 ggtcggcacc cgtgcttcgt gccaaccgat tccggtgttg aacatcgtga cgccggcggc
     2581 ttcgagcgcc tgggcgacct gcacggtttc ttcccaggtg ttgccgcctt ccaccagatc
     2641 gatcagcgag atccggtaca ccacgataaa gcgttcgccg cacaccgcgc gcacgcgctc
     2701 cacgatctcg cgcgcaagcc gcatgcggtt ctcgatgctg ccgccgtagc ggtcggtacg
     2761 ccggttcgtg cgcggacaca ggaactggtt gagcagatag ccttcgctgc ccatgatctc
     2821 gatgccgtcg tagccggcgc gttgggcgag gcgcgcgcag cgtgcgtagt cgcgaacggt
     2881 gcgcgcgatg ccggcttcgg tgagcgcgcg cggtttgaac ttcgaaatcg gcgacttgag
     2941 cgccgacgcc gacacgacga acggctgata cccatagcgg cctgcgtgca ggatctgcag
     3001 cgcgatcttg ccgccttcgg catgcacggc ctctgttagc aagcggtggt tgcgcagatc
     3061 gaacaccgag ttcagtgtgc cgccgaaggg cagcagccag cgttgccggt tcggcgagat
     3121 tccgcccgtg acgatcaggc cgaccccgcc tttcgcgcgt tcgcggaaat acgcggccag
     3181 cttcgggtaa ttccagaaac ggtcctccat gccggtgtgc atcgaaccca ttaccacgcg
     3241 gttgcgcaag cgggtgaagc cgaggtcgag ttcagcgagt aattgttggt aaggcatgga
     3301 agggaccgtg taagtattgg gtgcgcacga acaatacacc gctcatcgag ccaaccctcc
     3361 gaggaaaaat tctaatccac gcaacgcgtt gctccgtaag acattctgca ggatgcatac
     3421 gccatatcga accctgcggc acacgcattg ctgggtgcca gcgtccatcg acacggaccg
     3481 cgccactcac aaaaaacagg tgcgatgtgc agcagggata ggagggggca cttcatttcg
     3541 gagagccggg ctgaagccgt ttcaaagatc gacggatcag gtttcagacg gcgcgatgtc
     3601 cgaagcggag cgcccataaa ttaacgggtc gagcacccaa catcgatata ggtgaacaca
     3661 atgaaaatga tccgaaccat ggcagctgtg gttgcggtgg catccgtttt gaccgcatgc
     3721 ggcggcggct ccgacgacgt aggcaaggaa ctcggtttga ccaacccgga gattcatttc
     3781 atccacgcca ttccgggcgg tccggcagtc gactttctcg taaacggtaa agcgccatcg
     3841 ggccagacga atatcagcta caagggcgtg acgaacttca ccaatatcaa cacgggttcg
     3901 accactgtcg cctactcggc caccggcacg acgaccgcgc tggcgagcgg caacttcccc
     3961 gatgtggcga agggccatga atacacggtg ctggctttgc cgggcctggc tgcgccggat
     4021 atcggcctga tcgacgatcc gttcgacaag ggtctgctgt ccaacagcgc gcgcctgcgt
     4081 ggcttcaacg catcggctaa cgcgaccaac ctcgacttct atctggttca ggcaaccagc
     4141 actgacatca gcaaggtcag ccccaccatg gcgggcgtct cgttcaagaa cgcggtgccg
     4201 gcaagcggcc aggattcgat ttatgtttcc gggggccagt atgtgctgat cgcgacggtg
     4261 gcgggcagca agacgccgat tttccagtcg cagtctttct cgctgtcgaa caacgccgac
     4321 tggctggtga cgtccgtgcc gatcgccggg acgctgagcc agctcgtgcc ggggcagatc
     4381 catctgctga ttgcacagaa cggcaatacg agcacgccta gcgtcgagct ggcgaatgcc
     4441 ctgacgggcc aataaggcgc gaatcaggcg tcaacaagcc cgcgaatcag catgcgtaca
     4501 tagcatgcga tacacacaat aagcgacagt gcctgggagg gcagagcggc gatccacttg
     4561 gagtggatcg ccgttttgtt tggcgggccg cccatggatc cggcagctcg tgccatgaaa
     4621 aaagcccgcc gcggtttccc gcgacgggcg gtggagatcg tgcattgcga ggcgccacga
     4681 ggcgccgcga ggcgcccggc aatcgattgg ttatccctga cttacttggc ctgttcggct
     4741 gagttcgtga tggcgttgcc acccttcgaa atgtcctgcc cagcccccgc catcgtgttg
     4801 caaccggcga gcgcagccgt ccctgcaatg agaagcaaag cgatcagtcg attcatgaaa
     4861 gttctccttg tcgagaatgc atcggttgac gtgcctatgt tttacggccc gtgctagctc
     4921 ggctagctgt cgtccgaccg gggcttacgc cagtcgatcc ggtctgattg catggtaaaa
     4981 aaagggcggg gatcgcgctg tcagttgcgc tgcgattttg ttgtaggcgt gctccggtgt
     5041 ttgcgtcggc gcgctcctac acgagtttct gcaacagtcg caaagcctta ttcgacggag
     5101 gttcgcgacg gcgttgacgg cggccgatcg atcgatggcg ttgccgtgct gacactcggc
     5161 atcaccacat ggatgtcggt gccgtgcggc acgccgcgct ggatatcgaa gcgcccgccg
     5221 tgcgcggcga cgcgttggcg catgccgagc aagccgtggg tgtgcgtgcg tttcagatcg
     5281 gcgggtgtga tgccgacgcc gttgtcggca atatgcaacg ccacctcgcc gtcgccgacc
     5341 tgcaaagcga tcgataccct cgaggcgtgc gcgtacttgg ccgcgttcgt cagcgactcc
     5401 tgcgccacgc gaaagagcgc gatctcggtt tgctcgtcga gctggatatc gtcggcgggc
     5461 agatgcgatt cgagtgtcca gccgttgcgc tgcgcggctt cgtcggcgag cgtgcgcagc
     5521 gcggtcacca gcccgaagtt cgccagtacg gtgggccgca tgtcctcgat gatacggcgc
     5581 ttcagcgcga tgccctggtc gagattggcg agtgcgcgct tgagtttctc cgagatgtcc
     5641 ggggcgctgt ccttcagttt gcggttggcc caggccacgt ccattttgct cgcggtcaga
     5701 atcgcgccga gttcgtcgtg cagttcacgc gcgagctggg ttttttcgtt ttcgctgacg
     5761 gcctgcaggt gccagcccag cgcttcgagc tgacgcgtgc gctcgttgac gagctggtcg
     5821 agctcctcct gttgcgtgat caactgacgc tgcacgcgcg cttgccgatc catctggatt
     5881 ccgagattgc gaaacagcag gatgaacagc acgatgttca gcgccgacag cccggccgcg
     5941 cacaacgtgg agatgcgctg gtcggcgcgg cttgcgtcga gggcgtgctg cgcgcggcgc
     6001 tcctcgttgt cgcgcagcag cgtgagggtg ctgtcgacca gggtggcgtc ggcgggattg
     6061 gcttcgctcg ctgtgccgca ggcggagaga tcgagcgtct gcggtaccgc gcgttgcaat
     6121 gcagcagcgc ccgcactgat gcgcgcatcg atcaacgcgg cgatttgcgt gaagctcgcg
     6181 agctcggcgc tgtccgccct gccgcctcgg tagaagctgc tcagttgttg ttcgttctgt
     6241 ctgatgcgca cggcggtcgc gcaaaagcct cgagcggacg cgtcccgctt cgtcagaaga
     6301 aaatcgctct ggtgtgcgct cagactggct agctgactgg cgagcgtgct cagttgcacg
     6361 gtgatgtcgc gcgcttcgag cgcggtttcg tattccgcgg caatgcgttc ccgcgcggtt
     6421 tcgagaatca cgagtccgcc caccgtgatg acgatcgcca cggtcatcgc gatcgcccaa
     6481 ctgcgattgc gcatccagtc gctcaggcgc gcggcggcgc ctggtttctg cgggttcggc
     6541 acattcggca cattcggctc ctggggtggg gcatcagtgt aacgttcgcc ggctcgagga
     6601 ttcgaggagg gatggcgtgc attgcccttt gagtcgcatc gtccgcgcct gagtcgccga
     6661 gacccgtggc gtaacgctgc cgtaatccaa tgtaagcgga ttctcacacg aaaaacggcg
     6721 tgcgccggaa tgcggccgcc gttgccgatc gataggatga acctgatcgt tcattgattg
     6781 attctggagg aagccatcat gttgaaatgg gcattgttct ttgccgtcgt ggcagtgatc
     6841 gccggccttc ttggctttac cggcgtagcc gcgggcgcgg cggcgattgc gaagttcctg
     6901 tttgtcgtgt tcgtgattct gtgcgtggtg ttcctcgtgc ttggtttcgt ggtgacgaag
     6961 aaaatcatcg attagcgcgc ccgatgaggc cggcgcgtgc cgttcccgcg atggtgaggg
     7021 agcggatcgt ttcaggtttg cgcttcagat ttgccttggg agcgcccagg ttcactcaag
     7081 cttcgttgta tgcagatacc aacaaggaga gtcgccatgc ttcactatgc catcgtcttt
     7141 ttcgtgattg cgatcatcgc cgccgtgttt ggttttaccg gtatcgcagc aggtgctgcg
     7201 gaaatcgcga agatcctgtt ctacattttt cttgtcgtgt tcgtggtcac gctgctgctt
     7261 ggcgtgttcc gaacgtagac gaatacgcgg tccttgaacc ggcgtatcag gcgcgattcg
     7321 tgcgtgatac gcaattcccc ccaataccgg ataaaggaga gaatcaatgg caaagtcttc
     7381 tggcgcccaa ggcgcagcat ctcaatccag cgatccgttc gtgatggata ttaaaaagat
     7441 ccgcgaggac gcgcgcaagc acatgtcgga tgggccggtc acgcaaagct acggtgcgga
     7501 ccgcgagaca gtgctgaagc tgctcaacga ctcgctggct accgaaatcg tctgcgtgct
     7561 tcgttataaa cggcatcact tcatggccaa gggcatcaac tccgaggcgg tggcggcgga
     7621 attcgcagag catgcggcgg aagagcagga acacgccgac cgtattgccg agcgcatcgt
     7681 gcagcttggc ggtgagccgg attttgcacc ggacggtttg aagacgcgtt cgcattcgga
     7741 atacaaggaa ggcgacaatc ttacggacat gatccgcgag aatctgatcg cggagcgtat
     7801 tgcgatcgat acgtatcgcg agatcgtgca ttatctcggc gagaaggacg tgacgacgcg
     7861 ccgcttgttc gaggaaattc tcgccgtcga agaagagcat gccgatgata tggcggatct
     7921 gctcgaaggc cgcgagtaac aagcccgaag cggacactgg gaaacgcgga cgtcaaggag
     7981 cggatgtcaa gaagagcgga cgtcgaggag cggacgtcaa ggagctgatg tcaaggagct
     8041 gatgtcaagg agctgatgtc aaggaacgga tgtcaaggaa cggatgtcag ggaacggatg
     8101 tcgataagcg tggacgtcat aagcgcggac gtcgagaagc ggatgtcgat aagcgtggat
     8161 gccgagaagc ggatgtcgag aaacgcctat cggtaagcgc cgggcggtcg caacgcgaat
     8221 tgtcgctttg gttgctgcgc ctatgcactc gcaaattgcc ggcgcgggag ggtctaaatg
     8281 gtttcccgcg ccggcaacgt tcgccgcttc tcctgatgtg gctttacatc ttcgaccgag
     8341 gtgctggcga aggtcattac aatgtctgca ttggagcaat cctgttgcac ggacacgaga
     8401 ccgatgattc gagtgttgat agctgacgac catgcgatcg tccgaggtgg cttcaggcag
     8461 ttcgtcgctg acgagccgga catgtgcgtc gcggccgaag ccgcgaccgg cgacgaaacc
     8521 attcgcctcg tgcgggagca ggcattcgac gtcgtgttgc tcgacatcgc gatgccggac
     8581 aagaacggca tcgacacgtt gcgtgtgatc aggcaggtgc gaccggagca gggcgtgctg
     8641 attctgtcgg gctatcagga gagccaatac gcgatcaatc tgctgcgcgc gggcgccaac
     8701 ggttacctca acaaagattg cgagcccgag gagattgtgc gggcgatccg cgcggtggca
     8761 cgcgggcacc gttacctgtc ggaggcggtg gctgacacgc tggccgataa tctcgacaaa
     8821 ccggctgccg ggcggccaca cgaagcgttg tccgagcgcg agtttcagat tttctgcaag
     8881 cttgcggcgg gacagattcc gactgagatt gcggaggaat tgcatttatc cgtgaagacc
     8941 gtgagtacat accgggctcg cgtgttggag aagatgcggt tggcgaacaa tgccgatctg
     9001 acttattacg cgatcaagaa cggcttgatc gaatagtgaa cgatcgagac acaccgatga
     9061 atagcgaatc gaaccctgtt gccgagccag ccagtcgtgc gccgttgcgc gtgttgctga
     9121 tcgaggattc gccgctgatt cgcagaagcc tggtcgaggc tatcgatgca tcgggtttgc
     9181 tgcgggtggc cgcgtatgcg gattctgctg atcaggcgat tgctttgttg agcgatgagg
     9241 cttttgatgc ggtgattgtg gatttgcagc tgaagcaggg gtcaggggtg ccggtgctcg
     9301 cttatttgca gcgcgagggg atgatcgatt ccattttcgc tgccgtgttg acgaatcatg
     9361 cgttgccggc ttatcgggag cgttgtgagc aatatggtgt tcggcacttt tacgataagt
     9421 cgttcgagtt tgatcgggtg attgatgcgt tacatgagta tgcttttgcg cggggcgctg
     9481 ggtagggggt gtttttgcct gtgcggcgct tgttcgtctg tgtgcctggg gtgttggcct
     9541 ttccttgatt tgttattggt ctattagcgt cgcccctgtg cggggcgggc acctactttt
     9601 ctttgcctgc cgcaaagaaa agtaggcaaa agaaagcggc tcaccccgcc agctcataag
     9661 cgggtccccc gcaccgtt
//
LOCUS       DATASH010000350        12159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5787101,
            whole genome shotgun sequence.
ACCESSION   DATASH010000350 DATASH010000000
VERSION     DATASH010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 12159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12159
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5787101"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /gene="gshB"
                     /locus_tag="VHV99_09805"
     CDS             <1..928
                     /gene="gshB"
                     /locus_tag="VHV99_09805"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010090583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEX3635767.1"
                     /translation="TQFKIYKDSTYAMMAEAARRGHTLYTCEPKHLAWTGGDVEANVQ
                     RFAIVGDATDLHREPWYAAEAATPKSLKSFSAVVMRKDPPFDMEYVTSTWLLEMAERG
                     GARIFNKPQAIRDHSEKLAIGEFAQFVAPTLVTRDATRLRAFHEEHGDVILKPLDGMG
                     GMGVFRVKADGMNLGSIIEMLSHDGARSVMAQKFIPEIKDGDKRILLIGGEAVPFSLA
                     RIPQGNEVRGNLAAGGLGVARPLTEHDRKIADALAPVLAARGLLLVGLDAIGDWLTEV
                     NVTSPTCFREIMDQTGFDVAAMFIDALERAAA"
     gene            1266..1793
                     /locus_tag="VHV99_09810"
     CDS             1266..1793
                     /locus_tag="VHV99_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HEX3635768.1"
                     /translation="MAGILIIAHAPFATALRDCISHIYGGLPARIGVIDVSPDCDPAQ
                     QVAFAHSEIERLKEENGALVLTDMFGATPANIAGRLASVPNVRVLCGVNLPMLVRAVC
                     YRATPLDTLVDKALAGATKGVHAIGPATPAPVAPCAESGDCLPALPPEAASADCLPAL
                     PPEADLTQKAGSTGL"
     gene            1922..2191
                     /locus_tag="VHV99_09815"
     CDS             1922..2191
                     /locus_tag="VHV99_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010101417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEX3635769.1"
                     /translation="MLQQETTIVNKLGLHARASAKLTQLAGNYQAEIWMSRNGRRINA
                     KSIMGVMMLAAGIGSTVLIETEGTDEKEAMDALLKLIADKFGEGQ"
     gene            2488..4236
                     /gene="ptsP"
                     /locus_tag="VHV99_09820"
     CDS             2488..4236
                     /gene="ptsP"
                     /locus_tag="VHV99_09820"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEX3635770.1"
                     /translation="MSFTLHGIPVSRGIAIGRAYLIAPAALDVDHYLIEPAQIEGEIE
                     RFRAAQQHVHYELDALRADLAADAPSEMGAFINVHSMILNDAMLVQETIDLIRTRRYN
                     VEWALTEQLERLSRHFDDIEDEYLRERKADIEQVVERVLKALAGASVALVDGVHGTCD
                     EMIVVAHDIAPADMMQFKTQTFQGFVTDLGGRTSHTAIVARSLGIPAAVGVQHASQLI
                     RQDDLIIVDGDHGIVIVDPAPIVLEEYSYRQSEKALEQRKLQRLKFSPTQTLCGTRIE
                     LCANIELPEDARAAVDSGATGVGLFRTEFLFMNHKHQLPEEEEQFAAYRRAVELMNGM
                     PVTIRTIDVGADKPLDSMGGGDGYETAPNPALGLRAIRWSLSEPQMFLTQLRAILRAS
                     AYGKVKILIPMLAHAQEIDQTLDLIREARRQLDDAGIAYDPTVQVGAMIEIPAAAIAL
                     PLFLKRLDFLSIGTNDLIQYTLAIDRADNAVAHLYDPLHPAVLHLIAFTLREAKRAGV
                     PVSVCGEMAGDPALTRLLLGMGLTEFSMHPSQLLVVKQEVLRSHLKTLEKPVADVLAS
                     FEPEEVQAALKRVVLA"
     gene            complement(4318..5076)
                     /gene="moeB"
                     /locus_tag="VHV99_09825"
     CDS             complement(4318..5076)
                     /gene="moeB"
                     /locus_tag="VHV99_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEX3635771.1"
                     /translation="MNDDQLLRYSRHILVDEIGIEAQQRFIDAHAIIVGAGGLGSPAA
                     MYLAAAGVGRLTLVDADTVDLTNLQRQILHVSASVGRKKVESGRDAIAQINPEVVVNA
                     VAERVDDAWLDCEVPHATVVLDCTDNFATRHAINRACVRHGVPLVSGAALRFDGQIST
                     FDFRDAASPCYACVFPEDQPFEEVACSTMGVFAPTVGIIGSMQAAEALKVIGEIGTPL
                     VGRLTMLDSLRMEWNTMRIARQPDCPVCGQRHQV"
     gene            complement(5289..6875)
                     /locus_tag="VHV99_09830"
     CDS             complement(5289..6875)
                     /locus_tag="VHV99_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX3635772.1"
                     /translation="MRKNLKNIGLIAAGLATGVFATLQLSASAQQPASAASSAVAPLP
                     LDQLRLFAEVFGQIKHEYVEPVDDKKLLTAAIKGMVSSLDPHSSYLDKTDYEELQEQT
                     KGRFAGLGIEISSEDGLIKVISPIDDTPASRAGIRPGDLITRINDKPVRGMTLDQAVK
                     QMRGDPGTKVTLTIFRKTDDRTFPLTVTRAIIKVQSVKMKILAPGYAYIRITSFQERT
                     TPDLAARLQDIARQQPNLKGLVLDLRNNGGGLLQSAVGVAGAFLPPNSVVVSTNGQIP
                     DSKQVYRDTYDNYRLQSFDSDPLKDEAPIFKTVPMIVLTNAYSASASEIVAGALQDQH
                     RALIVGKTTFGKGSVQTVRPMTADTALRLTTAYYYTPSGRSIQNKGIRPDIAVDQYAE
                     GDPDDALVTREVDYSNHLANTQDPNEKKEAEQREQERMDQLRQLEEQNDKKTPEQRQK
                     DRERKPVEFGSTDDYMLQQALNKLEGKPVQESKSLTERRLAQNKPATSASAPVAVKPT
                     QPVPGASSPAPAAAPATQSN"
     gene            complement(7182..7928)
                     /gene="gpmA"
                     /locus_tag="VHV99_09835"
     CDS             complement(7182..7928)
                     /gene="gpmA"
                     /locus_tag="VHV99_09835"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011658011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEX3635773.1"
                     /translation="MYKLVLIRHGESTWNKENRFTGWVDVDLTEQGNREAQQAGVLLK
                     ESGYTFDIAYTSVLKRAIRTLWHVQDQMDQMYIPVVHSWRLNERHYGALSGLNKAETA
                     SRFGDEQVLVWRRSYDTPPPALDPSDERAPYNDPRYAKVPREQLPLTECLKDTVARVL
                     PLWNESIAPAIKSGRKIVIAAHGNSIRAMVKYLDNISDDDIVGLNIPNGVPLVYELDE
                     NLKPIKHYYLGDQDAIAKAQAAVAQQGKAG"
     gene            8066..8491
                     /locus_tag="VHV99_09840"
     CDS             8066..8491
                     /locus_tag="VHV99_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX3635774.1"
                     /translation="MKFFTDYTNLVLIAIVLISGGLLLWPTISRRGRGGLSAAEATQL
                     INRRNAAVIDLRPSADFAKGHLPAARHLEFAELQAKVAQLVKNKNNPVLLVCQTGQQS
                     NKAARIVQDAGYAEVHVLEGGVDAWQKAGMPVVKQGVAK"
     gene            8488..8751
                     /gene="grxC"
                     /locus_tag="VHV99_09845"
     CDS             8488..8751
                     /gene="grxC"
                     /locus_tag="VHV99_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEX3635775.1"
                     /translation="MNKVIMYSTQVCPYCQMAERLLKSRGVEHVEKVLIDKDPARREE
                     MMTRTGRRTVPQVFIGETHVGGYDDLSALDRAGGLMPLLEATA"
     gene            8903..9373
                     /gene="secB"
                     /locus_tag="VHV99_09850"
     CDS             8903..9373
                     /gene="secB"
                     /locus_tag="VHV99_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013337912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEX3635776.1"
                     /translation="MSDENNQPFFNIQRIYLKDMSLEQPNSPGIFLEQEMPSVEVEVD
                     VKAERLADTVFEILVTGTVTAKVSDKVAFLIEAKQAGIFDIRNIPAEQIDPLVGIACP
                     TILFPYLRSNIADAITRAGFPPIHLAEINFQALYEQRLAQMGGQEGAAQNGVTH"
     gene            9395..10393
                     /locus_tag="VHV99_09855"
     CDS             9395..10393
                     /locus_tag="VHV99_09855"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011546282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX3635777.1"
                     /translation="MKVAVLGAGAWGTALAGHLAARHDTVLWAREPALVADLCASHEN
                     ARYLAGVALPASLRYEADLNAALDHALADDALCVVATPVAGLRGLFRAMHDAGKVPAH
                     VVWLCKGFEADSQLLPHQVVAAELPGHGSNGVLSGPSFAREVGQGLPVALTIASTSST
                     CRERTVAAFHHGAMRIYTGDDIVGVEVGGAVKNVLAIATGIADGLGLGLNARAALITR
                     GLAEMSRLGVTLGGRAETFTGLTGLGDLILTATGDLSRNRTVGVQLAAGRTLDDILGG
                     LGHVAEGVRCAQAVLAIARAHAIEMPITQAVCAVLFDGVAPRDAVSALLRRDSKAE"
     gene            10491..11039
                     /locus_tag="VHV99_09860"
     CDS             10491..11039
                     /locus_tag="VHV99_09860"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HEX3635778.1"
                     /translation="MLSFNNCTLEARVVDITTLAVDAIVNAANTSLLGGGGVDGAIHR
                     AAGKGLVCECATHGGCAAGDAKITRGYQLPAKHVIHAVGPVWQGGARGEADLLASCYQ
                     RSLEVAHQAHCTSVAFPAISCGVYHFPANEAVRIAVGTVLETLPRTPKIERVVFACFD
                     DAMLARYEAELKYRQAPPSKPV"
     gene            complement(11012..11482)
                     /gene="trmL"
                     /locus_tag="VHV99_09865"
     CDS             complement(11012..11482)
                     /gene="trmL"
                     /locus_tag="VHV99_09865"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006763321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEX3635779.1"
                     /translation="MFNVVLVEPEIPPNTGNVIRLCANTGARLHLIEPLGFPLDDAKL
                     RRAGLDYHEYAQMNVHADWAAFVAKEAPDPARMFAFTTRGSGRFHEHAFQSGDWFVFG
                     AETRGLPDTVLDQFANEQRVRLPMRPGNRSLNLSNTVAVVVFEAWRQTGFDGGA"
     gene            complement(11501..>12159)
                     /locus_tag="VHV99_09870"
     CDS             complement(11501..>12159)
                     /locus_tag="VHV99_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEX3635780.1"
                     /translation="SHKTLCTGCDEAYWNEGRLRCTVCAVPLSGTRWASQVQYRCADC
                     IGAPPPFEATFALADYRQPLDALALGLKFRARLMLAREFAQRLARLAKDSWVDACDKP
                     DVLAPVPLANKRLTERGYNQAWQIARPLARALNVRSDATLLHRVIHTAPQSRLDLDAR
                     RLNVGRAFRVVKPVRGLHVGIVDDVMTTGATLEALARTLKAAGARRVTNFIALRTPKN
                     "
BASE COUNT         2205 a         3678 c         3912 g         2364 t
ORIGIN      
        1 tacgcagttc aagatctaca aagattcgac ctacgcaatg atggccgaag ccgcgcgccg
       61 cggtcacacg ctctacacgt gcgagccgaa acatctcgcg tggacgggcg gcgacgtcga
      121 ggcgaacgtg cagcgctttg caatcgtcgg cgacgcgacg gacctgcatc gcgagccgtg
      181 gtacgccgct gaagcggcca cgccgaaatc gctgaaaagc ttcagcgcag tcgtgatgcg
      241 taaagacccg ccgttcgaca tggaatatgt gacgtcgacc tggctgctgg aaatggccga
      301 gcgtggcggc gcgcgcatct tcaacaagcc ccaggcgatt cgcgatcact cggaaaagct
      361 ggcgatcggc gagttcgcgc aattcgtggc gcccacgttg gtcacgcgcg acgccacccg
      421 tttgcgtgcc ttccacgaag aacacggcga cgtgatcctg aagccgctcg acggcatggg
      481 tgggatgggc gtgttccgcg tgaaggccga cggcatgaat ctcggctcga tcatcgagat
      541 gctgagccac gatggtgctc gctcggtgat ggcgcaaaaa ttcatccctg agatcaagga
      601 tggcgacaag cgcattctcc tgatcggcgg cgaagcggtg ccgttttcgc tcgcgcggat
      661 tcctcagggc aatgaagtgc gcggcaacct cgctgcgggt ggcctgggtg tggcgcgacc
      721 gctcaccgag cacgaccgca agatcgccga cgcgctcgcg ccggtgctcg ccgcgcgtgg
      781 cttgctgctg gtcgggctgg acgcgatcgg cgactggctc accgaggtga acgtgacgag
      841 cccgacgtgc ttccgcgaga tcatggatca gaccggcttc gacgtcgccg cgatgttcat
      901 cgacgcattg gaacgcgcag cggcttgagg ccaaacaagc ccttgaatgg ccgccatggc
      961 ccccaaactg tgtagtaaat ccgcatcaag cgaaacaccg gctttggcgc gaaaatgagc
     1021 ctgctacaat gcccgctctc ccgcgccaag ccgaatcgac cgcgtctgcc ggacgctcca
     1081 ggcacggttt gcggactgta agcgagcagt gtttccgcca cagtgccgtc gctacattcg
     1141 gcgccgcggg ccggtcgtca gatgatgcgt tccggccctt acgacccacg gccggtcgag
     1201 ggcgcttgcg aaccgggttt ggtccggttc atggttcggg gccgttcttt tgcagtaagg
     1261 ctgacatggc aggcattctg atcattgcgc acgctccgtt cgccaccgcg ttgcgcgatt
     1321 gcatttcgca catctacggt ggcttgccgg ctcgcatcgg cgtgatcgac gtatcgccgg
     1381 attgcgatcc cgcgcagcag gttgcgttcg cgcactccga aatcgagcgg ctcaaggaag
     1441 aaaacggcgc gctcgtgctc accgacatgt tcggcgccac gccggcgaat atcgccgggc
     1501 ggctcgcatc ggtgccgaat gtccgcgtgc tatgcggcgt caatctgccg atgttggtgc
     1561 gcgcggtctg ttatcgcgct acgccgctcg acacgctggt cgacaaagcg cttgccgggg
     1621 ccaccaaggg cgtgcatgca atcggacccg ccacgccggc gccggttgcg ccttgcgcgg
     1681 aatccggcga ctgtttgccg gctttgccgc ccgaggccgc ttcggcggac tgtttgcccg
     1741 ccttgccgcc cgaggccgac ttgactcaga aagccggctc gaccggcttg taagcgaatc
     1801 gcacgccttc aaacggttgc aatcggattg ctgcccgctt gcaaccgatt tacaaccgat
     1861 ttgcaactgg tttgcagttt cacctttcac cacaacactt acccgcgctt cggaccatca
     1921 catgctgcaa caggaaacga ctattgtgaa caagctgggg ctgcacgcgc gcgcgtcagc
     1981 caagttgacg caactcgcgg gcaactacca ggcggaaatc tggatgagcc gcaatggccg
     2041 ccgtatcaat gccaagagca tcatgggtgt gatgatgctg gccgcgggga taggcagcac
     2101 ggtgctgatc gaaacagaag gtaccgacga aaaagaagca atggacgcac tgttgaaact
     2161 gattgccgat aaattcggcg agggtcagtg attcgcgcgc tctccttgcc tgagcaaaat
     2221 gccgcggctg tccgcggctt ttttttagcg cgtgacaatc ggcgcgtgag aggtgcgagg
     2281 gcgcatctgc cggcaacacg tggagggttc acgttgccgg accgcaaggt ggaccatggc
     2341 gcattgtcgc gggtggttag ggtattttgt gttgcgctgc acacagccct aacgtatcta
     2401 cacggggcgc ggaatttata atggcgacga gagtctgaga gcattgcagc ggtttgccgc
     2461 ggcattacac aactagagga ggtgcgcgtg tccttcacgc tgcatggaat tcccgtgtca
     2521 cgcggtatcg ccatcgggcg ggcttatctg atcgccccgg ctgcacttga cgtagaccac
     2581 tatctgatcg aacccgccca gatcgagggc gagatcgagc gctttcgcgc ggcccagcaa
     2641 catgtgcatt acgagctcga cgcgttgcgc gccgatctcg ccgcggatgc gcccagcgaa
     2701 atgggcgcgt tcatcaacgt tcattcaatg atcctgaacg atgcaatgct cgtgcaggaa
     2761 accatcgacc tgatccgcac gcgccgctac aacgtggagt gggcgctgac cgaacaactc
     2821 gagcgcctgt cgcgccattt cgacgatatc gaagacgaat acctgcgcga gcgcaaagcc
     2881 gacatcgaac aggtagtgga gcgcgtactg aaggccctcg ccggcgcctc ggtggcgctg
     2941 gtggacggcg tgcacggtac ctgcgacgag atgatcgtgg tcgcgcacga cattgcgccg
     3001 gccgacatga tgcagttcaa gacgcaaacg tttcagggtt tcgtcaccga tctcggcggt
     3061 cgtacttcgc atacggcgat cgtcgcgcgc agcttgggta ttccggctgc ggttggcgtg
     3121 cagcatgcga gccaactgat ccggcaggac gacctgatca ttgtcgacgg cgatcatggc
     3181 atcgtgatcg tcgatcccgc gccgatcgtg cttgaggagt attcgtaccg ccaaagcgaa
     3241 aaagcgctcg agcaacgcaa gcttcagcgt ctgaaatttt cgccgacgca aacgctgtgc
     3301 ggcacacgca tcgaactatg cgcgaacatc gagttgccgg aagacgcgcg cgcggccgtc
     3361 gattcgggcg caaccggagt gggcctgttc cgcaccgagt tcctgttcat gaatcacaag
     3421 caccagttgc cggaagagga agagcagttc gccgcctatc gtcgcgcggt cgagttgatg
     3481 aacggcatgc cggtgacaat tcgcacgatc gacgtcggcg ccgacaagcc gctcgattcg
     3541 atgggcgggg gcgacggcta cgaaactgcc ccgaaccccg cgttggggct gagggcgatt
     3601 cgctggagcc tctccgagcc gcaaatgttc ctgacgcaac tgcgcgccat tctccgcgcg
     3661 tcggcgtatg gcaaagtgaa gattctgatt ccgatgctcg cgcacgcgca ggaaatcgat
     3721 cagacactgg acctgatccg tgaagccagg cgtcaactgg acgatgccgg catcgcctac
     3781 gatccgaccg tgcaggtcgg cgcaatgatc gagattccgg ccgccgcgat tgcgttgccg
     3841 ctgtttctca agcggctcga tttcctgtcg atcggcacca acgatctgat ccagtacacg
     3901 ctggcaatcg atcgcgcgga taacgcggtc gcgcatctgt acgacccgct gcatcccgca
     3961 gtgctgcatc tcattgcctt cacgctgcgc gaggcgaagc gggcgggtgt gccggtgtcg
     4021 gtgtgcggtg agatggccgg cgatccggcg ttgacgcgtc tgctgctcgg catggggctt
     4081 accgagtttt cgatgcatcc gagccagttg ctggtagtca aacaggaagt gctgcgctcg
     4141 catctcaaga cgctcgagaa gccggtggcc gacgtgctgg cttcgttcga accggaagaa
     4201 gtccaggcgg cgctcaagcg agtcgtgctg gcttgaaact gagagccccg attgggttcc
     4261 aatagaaaac gccgcacctg tgtgcggcgt ctttcatttc agcgtcttat tcaggcgtca
     4321 gacctgatgc cgttgtccgc ataccgggca atccggctgg cgcgcaatgc gcatcgtgtt
     4381 ccattccatt cgcaacgaat cgagcattgt caggcggccc accagcggcg taccaatttc
     4441 gccgatcact ttcagcgctt cagccgcttg catcgaaccg atgatgccga ccgtcggcgc
     4501 gaacacgccc atggtcgagc aagcgacttc ctcgaacggt tgatcttccg ggaacacgca
     4561 cgcgtagcag ggcgacgctg cgtcgcggaa atcgaaggtg ctgatctggc cgtcgaagcg
     4621 caaggccgcg cccgacacca gcggcacacc atgtctcacg cacgcccggt taatcgcgtg
     4681 gcgcgtggcg aagttatcgg tgcaatcgag cacgacggtg gcgtgcggca cctcgcaatc
     4741 cagccatgca tcgtcgacgc gttcggccac cgcgttcacc actacctccg gattgatctg
     4801 ggcgatcgcg tcgcgcccgg actccacctt cttgcgcccg accgacgcgc tcacatgcag
     4861 aatctgccgc tgcagattgg tcaggtcgac cgtatcggca tcgaccagcg tcaagcggcc
     4921 gacacctgcc gccgccagat acatcgccgc cggcgaaccg agtccgcccg cgccgacgat
     4981 gatggcgtgc gcgtcgataa agcgctgctg cgcctcgatg ccgatttcgt cgacgaggat
     5041 gtggcgggaa tagcggagga gttgatcgtc gttcatggcg gggcgcgtgg agtgtcgcgc
     5101 aatgagatgc agggccggtg gcctgccgtg cgcggtttcg ttattttaat cgcgcgggga
     5161 gcgggtgttc gtactggtgc cgagtattgc aagacatgcg gcaagacatg cggcaccgcg
     5221 tacggcagcg tgtcctgcta cctgtcttcg cagcctgttc ggctgcgaag acaggtcggg
     5281 tctgctgcct agttgctttg ggtagcaggc gccgcggccg gagccgggct cgaggcgccc
     5341 ggtaccggtt gcgtgggctt caccgcgacc ggcgcggagg ccgacgtggc cggcttgttc
     5401 tgcgccagac gccgctcggt cagcgacttc gattcctgca cgggcttgcc ttccagcttg
     5461 ttcagcgcct gttgcagcat gtagtcgtcg gtcgaaccga actcgaccgg cttgcgctca
     5521 cgatccttct gacgctgttc cggcgtcttc ttgtcgttct gctcttcaag ctgccgcagt
     5581 tggtccatgc gctcctgctc gcgctgttcc gcttccttct tctcgttcgg atcctgcgtg
     5641 ttcgcaaggt ggttcgagta gtcgacttcg cgggtaacca gtgcgtcgtc cggatcgcct
     5701 tccgcgtact gatcgaccgc gatgtcagga cggatgccct tgttctggat cgaacggccg
     5761 ctcggcgtgt agtaatacgc cgtggtcaga cgcagcgcgg tgtcggccgt catcggacgg
     5821 acggtttgca ccgagccctt gccgaacgtg gtcttgccga cgatcagcgc acggtgctga
     5881 tcctgcagcg cgcccgcgac gatttcagac gccgacgccg agtaggcatt ggtcagcacg
     5941 atcatcggca cggtcttgaa gatcggcgct tcgtctttca gcggatcgct gtcgaacgat
     6001 tgcaggcggt agttatcgta tgtgtcgcga tagacctgct tcgaatccgg aatctgaccg
     6061 ttggtggata ccacaaccga attcggcggc aggaacgcgc cagccacgcc gaccgcgctt
     6121 tgcaacagac caccgccgtt attgcgcagg tcgagcacga ggcccttcag gttcggctgt
     6181 tgacgcgcga tgtcttgcag ccttgcagcc agatcaggcg tagtgcgttc ctggaagctg
     6241 gtgatgcgga tataggcgta gcccggcgcc aggatcttca tcttgaccga ttgaaccttg
     6301 atgatcgcgc gcgttaccgt gagcgggaac gtgcggtcgt cggtcttgcg gaagatggta
     6361 agcgtgacct tggtgcccgg gtcgccgcgc atctgcttga cggcctggtc gagcgtcatg
     6421 ccgcgcaccg gcttgtcatt gatgcgggta atcaggtcgc ccggccggat gccggcacgc
     6481 gaggcgggcg tgtcgtcaat cggtgagatc accttgatca ggccgtcttc agacgaaatt
     6541 tcgatgccga gacccgcgaa gcgacccttg gtctgctcct gcagttcttc gtaatcggtc
     6601 ttgtcgagat acgacgagtg cggatcgagg ctcgacacca tgcccttgat cgcggcagtg
     6661 aggagctttt tatcgtcaac cggttcgacg tactcatgct tgatttgccc gaagacttcg
     6721 gcaaagagcc ggagttggtc cagcggcaac ggtgcaacgg cgctcgaggc ggcgctggcg
     6781 ggttgctggg ccgaggcgga aagttgcagg gtggcaaata cgccggtagc aaggcccgcg
     6841 gcaatcaggc cgatattttt caggttcttt cgcatagagt ctgttgcggt cggaagcgcg
     6901 tggcagcgtc gatagagatg gggacggaca agtataactg cacacccgcc cagtcagggc
     6961 gcggcgcaga caatcgtgat tcgacagcac gcgcccggcc tggttcgtag gatcggccct
     7021 gcgctgggga agtaatgtgc ggtaacagtc gaatgacggc cagaaaggcg cgcaacgggg
     7081 atgcgggacg cgccaacgag tctgtcttgc gggttggcgc gacctattta gccgccattg
     7141 cgctgcattt ctgacggcag catggcggca agatggcagg atcagcccgc cttgccttgc
     7201 tgggcaactg cggcttgcgc cttcgcgatc gcgtcctgat cgcccagata gtagtgcttg
     7261 atcggcttca ggttttcgtc cagttcatag acgagcggga cgccgttcgg aatattcagg
     7321 ccgacgatgt cgtcgtccga aatattgtcg aggtacttca ccatggcgcg gatcgagttg
     7381 ccgtgcgcgg cgatcacgat cttgcggccc gacttgatgg cgggtgcaat cgactcgttc
     7441 cacaaaggca gaacgcgcgc gaccgtgtct ttcaggcact cggtaagcgg cagttgttca
     7501 cgcggcacct tggcatagcg cggatcgttg tagggcgcgc gttcgtccga cggatcgagc
     7561 gccggcggcg gcgtgtcgta gctgcggcgc cagaccagca cctgttcgtc gccgaacctg
     7621 gaggcggttt ccgccttgtt cagacccgac aacgcgccat agtggcgttc gttcagccgc
     7681 cacgagtgca ccaccgggat atacatctgg tccatctggt cctgcacatg ccacaaggtg
     7741 cggatcgcgc gcttgagcac cgaggtgtag gcgatatcga acgtatagcc cgattccttc
     7801 aggagcacgc ccgcctgttg cgcttcacga ttgccctgct cggtcaggtc gacgtcgacc
     7861 cagcccgtga agcggttttc cttgttccac gtcgattcgc cgtggcggat gagaacgagt
     7921 ttgtacatga gattgccggt cggtggtaag gaagcggtaa agcgctgcgg gggtccttta
     7981 gaggagcgcc gccatcgcgt cagacggtta ttttataatg ggcggattgc ccttttcgat
     8041 ttctctcttt ttcggcggat tttccgtgaa gtttttcact gattacacaa accttgtact
     8101 gatcgctatc gtcctcatct ccggtgggtt gctgctgtgg ccgacgatca gccggcgcgg
     8161 ccgcggcggc ctgtcggccg ctgaagccac gcaattgatc aaccggcgca atgcggcggt
     8221 catcgatctg cgtccgtccg ccgactttgc caagggccat ctgcccgcgg cacggcacct
     8281 tgaatttgca gagttgcagg cgaaagtcgc gcaactcgtg aagaacaaga acaacccggt
     8341 gctgctggta tgccagaccg gccagcagtc gaacaaggct gcgcgtatcg tgcaagatgc
     8401 agggtatgcc gaagtccacg ttctcgaagg cggtgtcgac gcctggcaga aagccggtat
     8461 gccggttgtg aaacaaggag ttgccaagtg aacaaagtga tcatgtacag cacccaggtg
     8521 tgcccgtatt gccagatggc cgaacgtctt ttaaagtcgc gcggcgtcga gcatgttgaa
     8581 aaggtactga tcgacaaaga cccggcccgt cgtgaagaaa tgatgacacg gaccggccgc
     8641 cgtacggtgc cgcaggtgtt catcggcgag acgcatgtcg gcgggtacga tgacctgtcc
     8701 gcgctcgacc gcgcgggcgg tttgatgcct ctgctcgaag caactgcctg aattcgcgcg
     8761 ccagacggcg cgtgaacgag gcgaatgaca tacaagccgg cgcgcactcg ccttgtcagg
     8821 gcgggcggtg tgtcgcaacg atctggcggc ccgtgaagcc atgggccgcc gccatgcata
     8881 cctatcaggg aatcactcaa tcatgtccga cgagaataac cagccgttct tcaacatcca
     8941 gcgcatctac ctgaaggaca tgtcgctcga gcagccgaat tcgccgggca tcttcctcga
     9001 gcaggaaatg ccctcggtcg aagtcgaagt cgacgtgaag gccgagcgcc tcgccgacac
     9061 cgtgttcgaa atcctcgtca cgggcacggt cacggccaag gtgtcggaca aggttgcgtt
     9121 cctgatcgaa gccaagcaag ccggtatttt cgatatccgc aacattccgg ctgagcagat
     9181 cgatccgctg gtcggcattg cctgcccgac gatcctgttc ccgtacctgc gctcgaacat
     9241 cgccgacgcg atcacccgcg caggcttccc gccgatccac ctcgccgaga tcaacttcca
     9301 ggcgctgtac gagcaacgtc tcgcgcaaat gggcggccag gaaggcgcgg cgcaaaacgg
     9361 cgtcacgcat taacgcgtcc gttcggtgcc ggctatgaag gttgccgtcc tcggcgccgg
     9421 tgcatggggc acagccctcg ccggccatct ggccgcgcgg cacgacacgg tgctgtgggc
     9481 gcgcgaaccc gcgctcgtcg ccgatctctg cgcttcgcac gaaaacgccc gctatctggc
     9541 gggcgttgcg ttgccggcgt cccttcgcta tgaagccgat ctgaacgcag cgctcgacca
     9601 cgcgctcgcc gacgacgcgt tatgcgtcgt ggcgacgccc gttgccggtt tgcggggttt
     9661 gttccgggcg atgcacgacg cgggcaaagt gccggcgcat gtggtgtggt tgtgcaaagg
     9721 tttcgaagcc gactcgcagt tgttgccgca tcaggtggtc gcggctgaat tgccggggca
     9781 tggcagcaat ggtgtgctat cgggcccaag cttcgcgcgc gaagtcgggc agggcttgcc
     9841 ggtcgcgctg acgatcgcga gtacgtcatc aacgtgccgt gagcgcacgg ttgccgcgtt
     9901 ccatcatggc gccatgcgca tctataccgg cgacgacatc gtcggcgtgg aagtgggcgg
     9961 cgcggtgaag aacgtgctgg cgattgccac aggtatcgcc gatggtcttg gcctcggtct
    10021 gaacgcgcgc gcggcgctga tcacgcgcgg tctggctgaa atgtcacgcc tcggcgtgac
    10081 gctcggcgga cgggcggaga cgttcaccgg gttgaccggc ctcggcgatc tgatcctgac
    10141 cgccacgggc gacctgtcac gcaatcgcac ggttggcgtg cagctcgcgg cgggccgtac
    10201 gctcgacgat attctcggcg gtctcggtca cgtggccgaa ggcgtgcgtt gtgcgcaagc
    10261 cgtactggcg attgctcgcg cccatgcgat cgaaatgccg atcacgcagg cggtctgtgc
    10321 cgtgctgttc gacggtgtgg caccgcgcga cgccgtcagc gccttgctgc ggcgcgattc
    10381 gaaagctgaa tgagtcttgt ctttcagccg tttagcgcga aaaagcgctg aacggctgcc
    10441 tactatcgtt gtccacgttt tatcgaggcg tcggatcatc gaggtttgcc atgctcagtt
    10501 tcaacaactg cacgctagaa gcgcgcgtgg tcgacatcac gacgctcgcc gtggatgcga
    10561 tcgtcaatgc cgcgaatact tcattgctag gcggcggcgg tgtggatggc gcgattcacc
    10621 gcgcggccgg caaggggttg gtgtgcgagt gtgcgacgca cggcggctgc gcggcaggcg
    10681 atgcgaaaat cacccgcggc taccaattgc cggccaaaca cgtgatccat gcggtcggcc
    10741 cggtttggca aggtggtgcg cgcggcgagg cggacctgct tgcctcgtgc tatcagcgct
    10801 cgctcgaggt cgcgcatcaa gcgcattgca caagcgttgc gtttcccgcg attagttgcg
    10861 gcgtctacca ttttcctgcc aatgaagccg tgcggatcgc cgtgggcacg gtgctcgaaa
    10921 ctttgccgcg caccccgaag atcgagcgcg tagtgttcgc ctgtttcgac gacgccatgc
    10981 tcgcgcgcta cgaagccgag ctgaaatacc gtcaggcgcc gccgtcgaag ccggtctgac
    11041 gccacgcttc gaacaccacc acggcgactg tattggagag attcaggctg cggttgcccg
    11101 gccgcatcgg cagacgcaca cgttgttcat tggcgaattg atcgagcacc gtgtcaggca
    11161 ggccgcgggt ttcggcgccg aacacgaacc agtcgccgga ttgaaacgca tgctcgtgaa
    11221 accggcccga accgcgggtg gtgaaggcga acatgcgcgc tggatcgggc gcttccttgg
    11281 cgacgaatgc tgcccaatcg gcatgcacgt tcatttgcgc atactcgtga tagtcgagac
    11341 cggcgcggcg cagcttggcg tcgtcgagcg gaaagccgag cggctcgatc agatgcagcc
    11401 gcgcgccagt gttggcgcac aggcggatga cgttgccggt gttcggcggg atttccggtt
    11461 cgacgagaac gacattgaac atgatggctt caggtggaga ctagtttttc ggtgtgcgca
    11521 gcgcgataaa gttggtgacg cgccgcgcac cggcagcttt gagtgtgcgc gccagcgctt
    11581 cgagcgtcgc cccggttgtc atcacatcgt cgacgatgcc gacgtgcaga cctcgcaccg
    11641 gtttcacgac ccggaatgcg cggcccacgt tcaagcggcg cgcgtccagg tcgagccgcg
    11701 attgcggcgc cgtgtggatc acacgatgca gcaaggtggc gtcgctgcgc acattcaacg
    11761 cgcgggccaa cggcctggca atctgccagg cctggttgta gccgcgctcc gtaaggcgtt
    11821 tattggccag cggcacgggc gcgagcacgt cgggtttgtc gcatgcatcc acccaggagt
    11881 ccttcgcgag acgcgcaagg cgttgtgcaa actcgcgcgc cagcatcagc cgggcgcgaa
    11941 atttcaggcc gagcgccagg gcgtcgagcg gctggcggta gtcggcaagg gcaaaggtag
    12001 cctcgaacgg cggcggcgcg ccgatgcaat ccgcgcagcg atattgcacc tgacttgccc
    12061 agcgcgttcc ggacagagga accgcgcaca ccgtgcaacg caaccggcct tcattccaat
    12121 acgcctcatc acaacccgtg cacagagtct tatgcgaca
//
LOCUS       DATASH010000351         2571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1237679,
            whole genome shotgun sequence.
ACCESSION   DATASH010000351 DATASH010000000
VERSION     DATASH010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237679"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="VHV99_09875"
     CDS             complement(<1..894)
                     /locus_tag="VHV99_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3635781.1"
                     /translation="MDRFKQIETFVTVAAKGSLSAAALAEGVAPAIIGRRIDALEERL
                     GVKLLVRTTRKITLTFEGSAFLEDCQRLIHDMQNAEASVSAGGVKASGHLRLSAPAGF
                     GRRHVAPLVPAFTVAHPDVSITLDLSDRLVDLVNEGFDCAVRLGELPDSSLVSLKLGE
                     NRRVCVASPSYLSRRGSPHTLADLAHHNCLALGASANQQRGWMFQQGDKVVSIKVSGT
                     MECSDGAVLHEWCLEGYGLAWRSWWEVGTDIAAGRLVSVLDEFAAPPIGIHAVFPQRR
                     HLPLRVRLFLDFLKHTYGHPDH"
     gene            1070..1957
                     /locus_tag="VHV99_09880"
     CDS             1070..1957
                     /locus_tag="VHV99_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-2-haloacid dehalogenase"
                     /protein_id="HEX3635782.1"
                     /translation="MSAITTPFPKAVIFDAYGTLFDVHSVIAAAEQMFPGQGDALSQL
                     WRLKQIEYTQLRTLAAPPDAPGAHYRPFWDITLDALRFATKKLHLTPGRAAEKRLMDE
                     YACLSAFPDAVPTLRQLRDGASSASAPVASAPAASTLAASASTASTGGKAGTRPGLAI
                     LSNGNPRMLDIAVKSAGMTGLFDHVLSVDTVRAYKPSPAAYALGTQAFGAEPHEIVFV
                     SSNGWDVAGASWFGFTTFWLNRQNAPAEELGVTPHGAGAGMNDLPAFLKSLASPDHHS
                     GTGSTGHPGGRRTRHSPRA"
     gene            2041..>2571
                     /locus_tag="VHV99_09885"
     CDS             2041..>2571
                     /locus_tag="VHV99_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HEX3635783.1"
                     /translation="MANPLSSQSSLQLPQGMDTTAEIKPGYDAILTREALELVAALHR
                     TFEPRRQQLLQARVERTKRLDAGERPDFLAETKNVREGDWKIAPLPQDLRCRRVEITG
                     PVERKMIINALNSGADSYMTDFEDSNAPSWDNLITGHINLKDAVRRTISLAQNGKSYQ
                     LNDKIATLIVRPRGWHL"
BASE COUNT          517 a          853 c          764 g          437 t
ORIGIN      
        1 gtgatcagga tggccgtacg tatgcttgag aaagtccaga aacaaccgaa cccgcagcgg
       61 caaatgccgg cgctgcggaa aaaccgcatg aatgccgatc ggcggcgcag caaattcatc
      121 gagcacgctg accagccgcc cagccgcgat atccgtgccg acctcccacc acgagcgcca
      181 tgcaagccca tagccctcga ggcaccactc gtgcagcacc gcgccgtccg agcattccat
      241 cgtgccggat accttgatcg acacgacctt gtcgccctgc tggaacatcc agccgcgctg
      301 ctgattggcg ctcgcgccga gcgcaaggca gttgtgatgc gcaagatcgg ccagcgtgtg
      361 cggcgagccg cgccggctca gatacgacgg cgaagccacg cacacgcggc ggttctctcc
      421 gagcttgagc gacaccagcg acgaatcggg caactcgccc agacgcacgg cacagtcgaa
      481 tccctcgttg acgagatcga ccagccggtc ggacaggtcg agcgtaatcg acacatccgg
      541 atgcgcgacg gtaaaggccg gcaccagcgg cgcaacatgc ctgcgcccga acccggccgg
      601 cgccgacagc cgcaaatggc cgctcgcctt cacgccgccc gccgatacgc tcgcctcggc
      661 gttctgcatg tcgtggatga ggcgctggca atcctcgaga aacgccgacc cctcgaaggt
      721 caacgtgatc ttgcgcgtgg tgcgcaccag caatttgacg ccgaggcgct cttcgagcgc
      781 gtcgatgcgg cggccgatga tcgcgggagc gacgccttcg gcgagcgccg cggcggacag
      841 gctgcccttg gccgcaacgg taacgaaagt ctcgatctgc ttgaaacggt ccatgggaag
      901 gagccgcaac gcccggaaga aaggaagccc cttgggagcg cgctggaagt ggtcggtgcg
      961 cgtcagatta agttcataaa agtaaaagat caagtgatct ttagcctatt ttttagcctg
     1021 gagtgtcact aatacaatcc gtcctgacct ctgataggag tgcacggcaa tgtcggccat
     1081 aacgacaccc ttccctaaag cagtgatttt cgacgcgtac ggcacgcttt tcgacgtgca
     1141 ttcggtgatc gctgccgcgg agcagatgtt cccgggccaa ggcgatgcgc tctcgcagtt
     1201 gtggcgtctg aagcagatcg aatacacgca gttgcgcacg ctcgccgcac cgccagacgc
     1261 gcctggcgct cattaccggc cgttctggga catcacgctc gatgccttgc gcttcgccac
     1321 caaaaaactg catctcacgc caggccgcgc ggcagagaag cgtctgatgg atgaatatgc
     1381 gtgcctgtcc gcttttcccg atgccgtgcc cacgctgcgt caactgcgcg acggggcttc
     1441 gtctgcttcg gctccggttg cttcggctcc ggctgcttcg actttggctg cttcggcttc
     1501 gactgcttcc acgggcggca aagccggcac acggcccggt ctcgcgatcc tctcgaacgg
     1561 caatccgcga atgctcgaca tcgccgtgaa gagcgccggc atgacgggcc tgttcgacca
     1621 cgtgctatcc gtcgacacgg tccgcgccta caaaccctcg ccggcggcct atgcgctcgg
     1681 cacgcaagcc ttcggcgcag agccgcacga gatcgtcttc gtgtcgtcga acggctggga
     1741 cgtggcaggc gcgagctggt tcggtttcac cactttctgg cttaaccggc aaaacgcacc
     1801 agccgaagaa ctgggcgtca cgccgcatgg cgcgggcgca ggcatgaacg atctgcccgc
     1861 ttttctgaag tccctcgcgt cgcccgacca ccatagcggc acgggcagca ccggtcatcc
     1921 cggaggccgc cgcacgcgcc acagccctcg cgcatgacgg ctaccgcctg aatcccacca
     1981 ggcatttcaa ccgcatctca acatttgcac cttcgcaatc tgaccgacca aggagaaatc
     2041 atggcgaatc cgctgtcatc gcaatcatcg ctgcagttgc cgcagggcat ggacaccacg
     2101 gctgagatca agccgggcta tgacgcgatc ctcacccgcg aagcgctcga actcgtcgcc
     2161 gcactgcatc gcacgttcga accgcgccgt cagcagttgt tgcaggcgcg tgtggagcgt
     2221 acgaagcggc tcgacgccgg cgagcgtccc gacttcctcg ccgaaacgaa aaacgtgcgc
     2281 gaaggcgact ggaagatcgc tccgctgccg caagatctgc gatgccgtcg cgtggaaatc
     2341 accggaccgg tcgagcgcaa gatgatcatc aacgcgctga attcaggcgc ggattcgtac
     2401 atgaccgact tcgaagattc gaatgcgccg agctgggata acctgatcac tggccacatc
     2461 aatctgaagg acgcggtgcg ccgcacgatt tcgctcgcac agaacggcaa gtcgtaccag
     2521 ttgaacgaca agatcgccac gttgatcgtg cgtccgcgcg gctggcatct g
//
LOCUS       DATASH010000352         6836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1634261,
            whole genome shotgun sequence.
ACCESSION   DATASH010000352 DATASH010000000
VERSION     DATASH010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6836
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1634261"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /locus_tag="VHV99_09890"
     CDS             complement(<1..951)
                     /locus_tag="VHV99_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007584182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX3635784.1"
                     /translation="MSETPQLRNGGRILVDALAHNGADTVYCVPGESYLAVLDALHDA
                     HGIRTITTRHEGAASNMADAYGKLTGRPGICFVTRGPGATHASNGVHTAREDSTPMIL
                     FIGQVERGFIGREAFQEVDYRQMFGGIAKWVTQIDSIERIPEIIAKAYSVAMSGRPGP
                     VVVALPEDVLFNSAEVNDALPVRIAQAAPEPQAMDALHALLAQAERPLVIVGGTGWNA
                     DACAALKRFVHANRLPVAASFRRQDLFDNRDPHYAGQLGLGVSPKLAQRARDADLLIV
                     IGSRLAEITSSGYTVFESPVPKQTMIHVHADADELGRVYQA"
     gene            complement(944..2185)
                     /locus_tag="VHV99_09895"
     CDS             complement(944..2185)
                     /locus_tag="VHV99_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007732285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3635785.1"
                     /translation="MQPWYSTMAPAQRRTFWACFLGWVLDAMDVQFFAFVIPTLLTTW
                     GMTKAQAGVIGTSALVSSAIGGVVAGVLADRIGRVRVLKLAILWFSLFTGLSAFTNSF
                     HELLLTRSLQGLGFGGEWAAGAILIGEVVDKRIRGRAVGSVQSGWPVGYALAALAYWV
                     LFHLLPEHLAWRVLFVIGLLPGLLVLWMRRNIDESQAFEAAAAYRDKRSSFETFANIF
                     ARDVISRTVLASLMAAGALGGNYTILTWLVTYLRETQSLTVNLTTLYLAVNIFGSFCG
                     YIGMAYLSDGIGRRWTFALSAAGATLTVLAYTLLHLPLSTLLVLGFPLGLFQSGIVSG
                     MGACFTELFPAHIRGSAGGFSYNFGRGIGALVPAAVGMTSASVGLGESIGVWAAVSYV
                     LVFVVAVFLPETRNKELEVHV"
     gene            2778..4325
                     /locus_tag="VHV99_09900"
     CDS             2778..4325
                     /locus_tag="VHV99_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017777904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX3635786.1"
                     /translation="MIKGLSIRRRLALAMGFLGVLLIAGGTMGVSGVAMSNSDLQRLY
                     SNQLASSVALSETSIALARARLWIFRIALNPAGSGVSKFAQNAQDQLDRSKNAWAAYR
                     ALPLSDADEERGAGEVDQRLKDLVTAGYEPVFQAIASADAGKITSAVSAMSAAQYGDV
                     TSRIDALEQTQAKLAQSSYARAEARFDWFVGIAIAGVAFALVAAALAWRSLQRAIGRP
                     IEEALHHFRAIAEGDLTARVEVRSSDEMGQMMNGLQAMQSKLVETISAVRDGARAIDT
                     AAREIAVGNMDLSSRTEEQAASLEQTSASMGELTTAVKHNTDNARQGNLLAVNASTTA
                     ERGGEMVRRVVETMHRISGSSKQVEQIIGVIEGIAFQTNILALNAAVEAARAGELGRG
                     FAVVASEVRSLAQRSASAAKEIKELIGQSAAHVNEGSKLVDETGGTIDEVVASAKRVA
                     SLMNEIAAASEEQHAGIEQVNRAVAQMDDVTQQNAALVEEASAAAQSLAAQSNGMREA
                     VTVFKLD"
     gene            complement(4977..5198)
                     /locus_tag="VHV99_09905"
     CDS             complement(4977..5198)
                     /locus_tag="VHV99_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEX3635787.1"
                     /translation="MAKKKTESVYRVTEIIGTSPISWEDAAKTAVETAAKTLRDLRVG
                     EVSKLDMKVESGKVVEYRARVSLSFKYEA"
     gene            5773..6210
                     /locus_tag="VHV99_09910"
     CDS             5773..6210
                     /locus_tag="VHV99_09910"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX3635788.1"
                     /translation="MRFIITAQASADNEEPNAGGGLDETLLTAYMRFNEEMHKAGVLV
                     ASEGLNPEAPGARIAVSNGKRCIVDGPFAESKELVGGFYIIEVGSLEEAISWALRAPS
                     GLGADDVLEVRQLTGAGDLPPNVLDIIRAAAPTWSESVWQAKN"
BASE COUNT         1418 a         2023 c         2153 g         1242 t
ORIGIN      
        1 cgcctgatag acgcgaccca gctcgtcggc atcggcatgc acgtgaatca tcgtctgctt
       61 cggcacgggg ctttcgaaca cggtgtaacc cgacgaggtg atctccgcga gccgcgagcc
      121 gatcacgatc agcaagtcgg cgtcgcgcgc acgctgtgcg agtttgggcg acacgccgag
      181 tccgagttgg cctgcgtagt gcggatcgcg gttatcgaac aggtcctgac gacggaacga
      241 cgccgccacc ggcaagcggt tggcgtggac gaaacgcttg agcgcggcgc aggcgtcggc
      301 attccagcca gtgccgccga caatcacgag cggacgctcg gcctgggcga gcaaggcgtg
      361 cagcgcgtcc atcgcctgcg gctcgggcgc cgcctgcgcg atgcgcacgg gtagcgcgtc
      421 attcacctcg gcggaattga acagcacgtc ctcgggcaaa gcgaccacga ccggcccggg
      481 acggcccgac atcgccacgc tgtacgcctt cgcgatgatt tcggggatgc gctcgatgct
      541 gtcgatctgc gtgacccact tcgcgatgcc gccgaacatc tgccggtaat cgacttcctg
      601 aaacgcctcg cgtccgataa agccgcgctc cacttgcccg atgaacagaa tcatcggcgt
      661 cgagtcttcg cgggcggtgt gcacgccgtt gctcgcgtgc gtcgcaccgg ggccgcgcgt
      721 cacgaagcag atgccggggc ggcccgtgag cttgccgtac gcatccgcca tattcgaagc
      781 ggcgccttca tgacgggtcg tgatggtgcg gatgccgtgc gcgtcgtgca acgcatcgag
      841 cacggccaga taactttcgc cgggcacgca gtagacggta tcggcgccgt tgtgcgcaag
      901 cgcgtcgacg aggatgcggc cgccgttgcg cagttgtggg gtttcagaca tggacttcaa
      961 gctccttgtt acgggtttct ggcaggaaca cggcgacgac gaagacaagc acgtaggaga
     1021 cggcagccca cacgccgatc gactccccga gtccgaccga tgcactcgtc atgccgacgg
     1081 ccgcgggcac gagcgctccg atgccccggc cgaagttgta cgaaaagccg cccgccgatc
     1141 cacgaatgtg cgcgggaaac agctcggtga agcaggcacc catgcccgaa acgatgcccg
     1201 actggaataa tccgagcggg aagccgagca cgagcagtgt ggagagcggc agatgcagaa
     1261 gggtataggc cagcactgtc agcgtcgcgc cggctgcgga cagtgcgaaa gtccaacgtc
     1321 ggccgatgcc gtcgctcaga tacgccatgc cgatatagcc gcagaacgag ccgaagatgt
     1381 tgacggccag atagagcgtc gtgaggttga ccgtcaggct ttgcgtctca cgcagatacg
     1441 tgacgagcca cgtgaggatc gtgtaattgc cgccgagcgc gcccgccgcc atcaatgaag
     1501 caagcacggt gcgcgagatc acgtcccgcg cgaagatgtt cgcaaaggtt tcgaaagaac
     1561 tgcgcttgtc gcggtaagcg gcggcggctt cgaatgcttg cgattcgtcg atgttacggc
     1621 gcatccagag cacgagcagg ccgggcaaga ggccgatcac gaagagcacg cgccacgcga
     1681 gatgttcggg caacaggtgg aacagtaccc agtaagcaag cgccgccaaa gcgtagccga
     1741 ccggccagcc gctttgcacc gagccgaccg cgcggccgcg aatgcgcttg tcgacgacct
     1801 cgccgatcag gatcgcgccc gccgcccatt cgccgccgaa gccgagacct tgcaggctgc
     1861 gcgtgagaag cagctcatga aaactgttcg taaaggctga aagcccggtg aagagcgaga
     1921 accacaggat cgcaagcttc agtacgcgca cgcggccgat gcggtccgcg agcacgccgg
     1981 cgacgacgcc gccgatcgcc gacgaaacca gcgccgaggt gccgatcacg ccggcttgcg
     2041 cttttgtcat gccccacgtg gtcaacaagg tcggaatgac gaatgcgaag aactgcacgt
     2101 ccatcgcatc gagaacccag ccgagaaagc acgcccagaa agtccggcgc tgggcgggcg
     2161 ccatggtgct gtaccaaggt tgcatgaggt gtatctccat caggcagagg gcgcctacca
     2221 tttttgtcgt cgtgcgcggc ggatgcgtcg aaagaagccc gcaccgagtt gcgcgtcaga
     2281 ttatgccgag gggtgttcga attgacctgt gcgcgagacg catagcagct atgcgcgaga
     2341 gcgtcggggc gccatcggct tgcgtccgtt caaccggttg atgatggggc gaacccctgc
     2401 tgacgggcct gagcggggcc agggcggcgg ccgcagtccc gcccacagga tcagtaagcg
     2461 tcacacgccg atcttaagtt tctagcaggc gggccgtaaa tcacgcatac gaagggatcg
     2521 tctattcgtg tcaataccgt ggttggagaa tagccaaatg ccgcaagtgc cggctactcc
     2581 atatgcaata gatagaggtg aaaatatgga tacggaggtt catatgcttc tcgaaagcaa
     2641 cctgtcatct ggaaggtcag aggcaatgta catcgccgct gagctggcat acgtggttgc
     2701 cgtttgcgcg acagccgttt ccgacatacc gttcccatgt caacgcaata aagacgtgac
     2761 ggccactttc ttccatcatg attaaaggac tttcaatccg ccgtcgcctg gcattggcga
     2821 tgggcttcct gggcgtcctg ctgattgccg gcgggacaat gggcgtcagc ggtgtcgcga
     2881 tgagcaacag cgacctgcag cggctctatt cgaatcagct tgcgtcgtcg gtcgcactca
     2941 gcgaaacaag catcgcactc gcgagagcgc gcctctggat cttccgcatc gcgctcaacc
     3001 ccgccgggtc cggcgtgtcg aaattcgcgc agaacgcaca agaccaactc gatcggtcaa
     3061 agaacgcgtg ggccgcatat cgcgcgttgc cgttgtcaga tgcggacgaa gagcgaggcg
     3121 ccggcgaagt cgatcaacga ttgaaagatc tcgtgacggc cggttacgag ccggtttttc
     3181 aggctattgc gtccgcggac gcgggcaaaa tcacatctgc cgtgtcggct atgtcagcgg
     3241 cgcaatacgg cgatgtcacc agtagaatcg atgcgctcga gcagacgcag gcgaagctcg
     3301 cgcagtcgtc ttatgctcgg gccgaggcgc gctttgactg gtttgtcggt attgcgatag
     3361 cgggagtcgc attcgcgctg gtggccgcag cgcttgcatg gcgatcgctg caacgcgcga
     3421 tcggccgccc gatagaggag gcgctccatc atttcagagc gattgccgag ggcgatctga
     3481 ccgcacgcgt cgaggttcgc tcatccgacg aaatgggtca gatgatgaac ggcctccagg
     3541 caatgcaaag caaactggtc gaaacgatca gtgcggtgag agacggtgcg cgcgccatcg
     3601 ataccgcggc cagggaaatt gcagtcggca acatggatct ttcctcgcgc accgaagagc
     3661 aggccgcatc gctcgagcag acgtccgcca gcatgggcga actcacgacg gccgtgaagc
     3721 acaacaccga taacgcacgt caggggaacc tgcttgcggt gaacgcttca acgaccgccg
     3781 agcgcggcgg cgaaatggtc cggcgcgtgg tcgagacaat gcacaggatt tccgggtcgt
     3841 cgaagcaggt cgagcagatc atcggcgtga tcgaagggat tgcgttccaa accaatattc
     3901 ttgcgctaaa cgctgcggta gaagcggcgc gtgctggcga actggggcgt ggattcgcgg
     3961 tggtcgcttc cgaggtgcgc agcctcgcgc agcgcagcgc aagtgccgcg aaggaaatca
     4021 aggagttgat cggccagtct gctgcccatg tcaacgaagg ctcaaagctg gtggacgaga
     4081 ctggcggcac gatcgacgag gtagtcgcct cagcaaaacg cgtggccagc ctcatgaacg
     4141 agatcgccgc agcgtcggag gagcagcatg ctggcatcga acaggtgaac cgggcagtag
     4201 cgcaaatgga cgatgtcaca cagcagaatg ccgcgctggt tgaggaggca tcggcggcag
     4261 cgcagtcgct ggcggcgcag tccaatggga tgcgcgaagc cgtgacggtc ttcaagctgg
     4321 attaagcgct ttccagcagg actttttgac gttcacgcgg ccaactgcgg aatagatgat
     4381 ccagctattc cagcatccag cttcatccgg tgcctgcccc aaaatcggcg tcggttggcc
     4441 gaaattgctg tccagttggc agacaggcaa gtattcgcat gattgccgta aatactcgat
     4501 ctcgctacgc tttgttccgg ctgacgcgcc tgacgcaggt cttaccgtgt caaaaaggtg
     4561 gcttcatttg ccggagcatg gacaccgaca gagccggttg ataacgatta ccggaaggat
     4621 cagtcctcct tcacctgaag tcagtaactt tggccggcga cgggcagcgc gtgagttcag
     4681 cgaccggatt ggcaggttac gtaacctgcc ttggcggctg ctcctcacgc acacttcttg
     4741 ccaggttcgt ccagaccgcg ctgagcacgt cgatcaggaa gatcgcacag agcggattgg
     4801 gcaacgtatg gcgtctgaac gagagtgcgg ctgggtgcac cgcttgcagg cgcaaggcag
     4861 gcgttggaca aaaacagtta caaaaggcgg gaagcagatg ccctttgcgt ttgtcggtac
     4921 gctcaagcag ttcggccgcg atgtcctgtc gtgctttgcc acagtgagtc gccggttcag
     4981 gcttcgtact tgaacgacaa agacactcgc gcccggtatt caacgacctt gccgctttcg
     5041 accttcatgt cgagcttgct gacttcgccg acgcgcaaat cacgcagcgt cttggcggcc
     5101 gtttccacag ccgtttttgc cgcgtcctcc cacgaaatcg ggctcgtgcc gatgatttcc
     5161 gtgacacggt aaacggattc ggtcttcttc ttggccatgt tctcgctcct gataggaggc
     5221 gcaagtgacg cccctcgatg atttgatgat aggtcgtctg cgtggtaagt gtcgcttgaa
     5281 ttcgccgtag acgttggttt tcgcctgcgc ttcgtcaatc cggagatgga agctggctta
     5341 accgcaaggc cccaccgctg tttatcgaat tgcgggcgta cgggccgcct tctgcacggt
     5401 ttcgtcatgc ggcgacgggc gaggaccgtt aagggttatt cgacgcctgc caacacgcgc
     5461 agaaacaccg tcacattcct gtggccgatg gcaggcgtcg aataagcctt gacgaccggc
     5521 gccagatgcg gatcaatgac aggcaggcca caactccggg ttgacgttcg ctcgcggttg
     5581 cccactggct gcccttcgtg aacgtcttct ttcgggtacg ctcggatccc tgcgatttca
     5641 tgtctcacag aggcgctcgt catcggcctg atgggctgcg atacgaatca tcggaattgg
     5701 ccgcaacgtg tcgattcgac agcccttcgt tcgtcgtggt cctgaagcac cattcaactt
     5761 aggagaacga cgatgcgatt catcatcaca gcccaagcaa gcgcagacaa tgaggagccg
     5821 aatgccggcg gcggtttgga cgagaccctt ctcacggcgt acatgaggtt caacgaagag
     5881 atgcacaagg ccggtgtcct ggtggcttcc gaaggcttga accctgaagc gcccggagcg
     5941 cgaattgcgg ttagcaacgg gaaacgctgc atcgtcgatg gcccgtttgc cgagtccaag
     6001 gaactggtcg gcggttttta catcatcgaa gttggatccc ttgaggaggc gatttcctgg
     6061 gcattgcgcg cgccatccgg tctcggcgcg gacgatgtac tagaggtgcg ccagctcacc
     6121 ggagcgggcg acctcccgcc aaatgtcctc gacatcatcc gggcagcggc accgacctgg
     6181 agcgagtcgg tttggcaggc gaagaactga agaccacata cggaaccgcc gggttgccat
     6241 ccagcccggc gctcgagccg acgtacgcag cgaacctccg tctacggctc agcatcagcg
     6301 tagagcgagc gcttttcggc ggccataccc gcggggaacg accgtcggct tcagatcggc
     6361 ggcgttacgt tcggcctggc cgatttcagg ctggactcgg cccatgaacg gccattcgcc
     6421 cgcgtgccgg gacggatact cgaacggctg ttccgcccat aaagcgaccg ctccgcaaca
     6481 agaccaggcc gatcgatgaa ggtcgcacca actccggaca agtagtagtg gcaagaagat
     6541 gtggaacaat caacagacta atcaagtcaa ggtccacaga ttcccggcgc ttcccagact
     6601 cgatggattt gaggactgat ttcttgatgt acttcgaaac gacggtcgcg tacagcatca
     6661 ccttgcgccg accggaatcg atattaatcg acatgcgacc tggacattct ccgacgctat
     6721 tcaccgggcc ccgttggcga tagcggcggc aaggcagtct cgctgcgact gcagatgtga
     6781 ccaggcggtc ataagcgcgt cgagggacgc tagctccttg tcacgagatc ccaaac
//
LOCUS       DATASH010000353         7456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_445771,
            whole genome shotgun sequence.
ACCESSION   DATASH010000353 DATASH010000000
VERSION     DATASH010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7456
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445771"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VHV99_09915"
     CDS             <1..218
                     /locus_tag="VHV99_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635789.1"
                     /translation="GKRVKGMGGHTVERLQAEGLAVGPAGAAANTQAAASAQSKGAAA
                     AGNGANAANSTNAANAQKSNATVAVKK"
     gene            610..1512
                     /gene="cysM"
                     /locus_tag="VHV99_09920"
     CDS             610..1512
                     /gene="cysM"
                     /locus_tag="VHV99_09920"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006476564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HEX3635790.1"
                     /translation="MAYKTIEDTIGNTPLVQLVRLPDDEIRSRNNVILAKLEGNNPAG
                     SVKDRPALSMIKKAEARGRIKPGDTLIESTSGNTGIALAMAAAIRGYKMVLIMPEDLS
                     VERRQSMAAYGAQIVLTPVTGGMEYARDLAEQMQRDGKGIILDQFANPDNPAAHVEGT
                     GPEIWRDTEGRITHFVSSMGTTGTIMGVSTYLKEQNQAIEIIGAQPEEGSRIPGIRKW
                     PEAYLPKIFDRSRVDHVENVSQAAAEAMARRMASVEGIFAGISSGGACEVALRVARQV
                     ENATIVFIVCDRGDRYLSTGVFPA"
     gene            complement(1873..3021)
                     /gene="mltB"
                     /locus_tag="VHV99_09925"
     CDS             complement(1873..3021)
                     /gene="mltB"
                     /locus_tag="VHV99_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HEX3635791.1"
                     /translation="MFMATSPALAQSTAKKPPVLLAQSQPQPAVPQGQTFEEEIIPQR
                     YANNPNVDAFINDMSARYDFDEAALHALFARVSYSATAVKLVTPSPSPSIKNWRVYQS
                     RFLDQVRINAGVRFWRANQATLQRAYEEFGVPPEVIVGIIGVETIYGRFMGNFRVLDA
                     LTTLSFDYPNTANRADRQATFRKNLEDYLVWTRDSQIDPTSVLGSYTGAIGIPQFLPS
                     SIVQYAVSYDGNRQIDLRTNQADAIGSVANYLRQNGWENGRPVVWKIGTDAGSLGVAQ
                     AAADGQPEPHWPLNQLLRAGLLLNEPALDIASEASTPVTVVDLPSPGRGTEYMLGLKN
                     FYVLTRYNRSFFYALAVYQLGQRVKAQMEASDAANASSNAAAPDAASQ"
     gene            3305..4228
                     /locus_tag="VHV99_09930"
     CDS             3305..4228
                     /locus_tag="VHV99_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEX3635792.1"
                     /translation="MATGFYSHADCLLHDMGQWHPECPARLQAIEDQLIASRIDSLIE
                     RESPPLADEAALLRVHTQAHVDYIRSRSPAEGLAEIDPDTSMNPYTLQAALRAAGAAV
                     AATDAVIEGRYDNAFCSVRPPGHHAEPARAMGFCFFNNVAIAARHALEVHGMQRVAII
                     DFDVHHGNGTEAAFSGDSRVLMCSIFQHPFYPFTGADNQAPNMCNVPMPARSKGMAVR
                     EAVDLLWLPRLHDFKPEMIFISAGFDAHREDDLGNMGLVEDDYAWITDQVREIAKRYA
                     DGRIVSCLEGGYNLSALGRSVVAHVRSLAGI"
     gene            4302..5102
                     /locus_tag="VHV99_09935"
     CDS             4302..5102
                     /locus_tag="VHV99_09935"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX3635793.1"
                     /translation="MNAEVRSASLVETEHDAYGVPGVVRLTMNRPDAFNSLSEALLDE
                     LQATLTALAQSSARVVVIAGAGRAFCAGHDLKEMRAAPSLAYYQALFARCTKLMLTIQ
                     RLPQPVIARVHGIATAAGCQLVAMCDLAVAADTARFAVSGVNLGLFCATPSVPLSRNL
                     SRKAALEMLLTGDFIDAVQAKHQGLVNRVVPADALDTELARLAKSICAKPVEAVRAGK
                     GLFYRQLEMGIEAAYQLAGQTMACNMMDESALEGVQAFIDKRPPDWKR"
     gene            complement(5257..6171)
                     /locus_tag="VHV99_09940"
     CDS             complement(5257..6171)
                     /locus_tag="VHV99_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3635794.1"
                     /translation="METTRPGNVRPAAGAPAGAPKGGAALRSTITTSDGIELPLYRWP
                     ATTQLRATVALVHGLAEHAGRYAALADRLNPAGIELVAIDLRGHGHAPGRRVWVNRFD
                     DYLLDAQALLDEAAISCAPLFLMGHSMGGAVAALYAIERLEASGRRLSGLILSSPALA
                     PGRDVPRWMLKASQIISRIWPTFPAMKIDAGLLSRLQPVVNANRNDPLVHHSAIPART
                     GAELLRAMARIERGRAGLRVPLLVYHGTADKLTEPEGSRDFGAHAGSPDKTLTLHEGS
                     YHETMNDLDRDRVIGALTDWIVKRLPRA"
     gene            6423..>7456
                     /locus_tag="VHV99_09945"
     CDS             6423..>7456
                     /locus_tag="VHV99_09945"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEX3635795.1"
                     /translation="MIEIRNISQRFAGPRGWVEALHNVNLSIPAGEVFGIIGRSGAGK
                     STLVRTINLLTRPSEGNIVVNGRDLTTLPAAQLREARREIGMIFQHFNLLSSRTVYEN
                     VALPLELAGMKRDEIEANVLPLLELVGLSAQKDRYPAQISGGQKQRVGIARALASKPK
                     VLLSDEATSALDPETTRSILDLLKRINRELNLTIVLITHQMDVIKQVCDRVAVLDAGR
                     VVEEGKVIDVFLQPHHEVTRALIGDVIAQELPPAMKARVAERLKTGSGHLLRLAFTGS
                     GVDQPILSETIRRYELDFNILHGQIDEIQGQAFGSLAVLAGGEPANVAQALTYLREQG
                     VVVEELSYVE"
BASE COUNT         1337 a         2320 c         2426 g         1373 t
ORIGIN      
        1 tcggcaagcg cgtcaaaggc atgggcgggc ataccgtcga gcgcctgcag gcggagggtc
       61 tcgctgtcgg tccggctggc gcggctgcta acacgcaggc tgcggcgtct gcacagtcca
      121 agggcgcggc cgctgcgggc aatggcgcca acgcggccaa ctcgactaac gcggccaatg
      181 cccaaaagag caacgccact gtggcggtga agaaatagcc tgacgcggac cgtgcgcgcg
      241 aggccggtca cctccgcgcg cacggtgcgc catcgtagtt cccttcatgg tcggagtggt
      301 aagaacactc cttcagctgt catgcagatg actggctccc gcggttctca gccgcgggct
      361 ttttgcgtgg tttctcggtg tggcttgtgc gcggtttgtg cgctgctctt tgcacgggct
      421 tgtgcacgga cttagatgcg gacttatgcg cggccctcgc gatttttttg cgcggcgacg
      481 ggtcgccatg cacgctcgcc agtcagcgcc tgcatccatg cggccagcag ggcctcgcgg
      541 cagccacccg cacggtggtt tagaatcgat ggattgaata cttagcgcat cgacagcact
      601 cgacccgata tggcatacaa aacgattgaa gacacgattg gcaatacgcc gctcgtgcaa
      661 ctcgtccggc tccctgacga cgagatccgc agccgcaaca acgtgattct tgcaaagctc
      721 gaaggcaaca atcccgcggg ttcggtgaag gaccgcccgg cgctgtcgat gatcaagaaa
      781 gcggaagcac gcggccgtat caagccgggc gacacgctga tcgaatcgac cagcggcaac
      841 acgggtatcg ccttggcgat ggctgcggcg atccgcggct acaagatggt gctgatcatg
      901 ccggaagatc tctcggtgga gcgccgtcag agcatggccg cttatggcgc gcagatcgtg
      961 ctgacgccgg tcacgggcgg catggaatac gcgcgcgatc tcgctgaaca gatgcagcgc
     1021 gacggcaagg gcatcatcct cgatcagttt gcgaatccgg acaatccggc cgcgcacgtc
     1081 gagggcaccg ggccggaaat ctggcgcgac acggaagggc gcatcacgca cttcgtttcg
     1141 tcgatgggca caaccggtac gatcatgggc gtgtccacct atctgaagga acagaatcag
     1201 gcgatcgaga tcatcggcgc gcagccggaa gagggttcgc gcattccggg catccgcaaa
     1261 tggccggaag cctatctgcc gaagattttc gatcgcagcc gcgttgacca cgtcgagaac
     1321 gtgagccagg ccgcagcgga agcgatggcg cgccgcatgg cctcggtcga agggatattt
     1381 gccggcattt cgtcgggtgg ggcctgtgag gtggcgctgc gcgtcgcgcg ccaggtcgag
     1441 aacgcgacga tcgtgtttat cgtctgcgat cggggtgacc ggtatctgtc gacgggtgtg
     1501 ttcccggctt gattgcgctt gggcggatgg agtggccctc aagctgagtt agccgataaa
     1561 aaagcgccgg ttcaaccggc gcttcagact gctgaccaac ccccacgttt ttcggagcgt
     1621 ggggttttgt ctttcagggg aggcgaatcg ctgcggggcg tgggagtttg tgagtaagcc
     1681 gggccagacg tgcggtgatg agtcggtggt tgctgccatc attttgtgtc gctcgcgaac
     1741 ccgctcttaa agcgtttgcg acccgttcgc ggcgcgcttg caaccccgtt cgtgaaagcc
     1801 gcctgcaacc cactcgtgac ccgcctgcaa cccgttcgca acgcggcgag cgtccgggcg
     1861 ccggccctca gcttattgcg acgccgcgtc cggcgccgcc gcattgctgc tggcgttcgc
     1921 cgcgtcgctc gcttccatct gcgctttgac gcgctggccc aattgataaa ccgccagtgc
     1981 gtagaagaaa ctccggttat agcgcgtcag cacatagaag ttcttcaagc ccagcatgta
     2041 ttccgtacca cgccccggcg acggcagatc gaccaccgtc accggcgtcg aggcctccga
     2101 cgcgatatcg agcgccggtt cgttcagcaa caacccagcg cgcaacaatt gattcagcgg
     2161 ccaatgcggt tctggctgac cgtcggcggc cgcttgcgcg acgcccaggc taccggcgtc
     2221 cgtgccgatc ttccacacca ccggtcggcc attttcccag ccgttctggc gcagataatt
     2281 tgccacgctg ccaatcgcat ccgcctggtt cgtgcgcaga tcgatctgcc tgttgccgtc
     2341 gtagctcact gcgtattgca cgatgctgct cggcaggaac tgcggaatcc cgatcgcgcc
     2401 ggtgtaggag ccgagcacgc tggtcggatc gatttgcgaa tcgcgcgtcc acaccagata
     2461 gtcttcgaga ttcttgcgaa aggtcgcctg gcgatccgcg cgattggcgg tattcggata
     2521 gtcgaagctg agcgtggtca gcgcgtcgag cacgcggaag ttgcccatga agcgcccgta
     2581 gatcgtctcc acgccgataa tgccgacgat cacttccggc ggcacgccga actcttcata
     2641 ggcgcgttgc agcgtggcct ggttggcgcg ccagaagcgc acgccggcat tgatgcgcac
     2701 ctggtcgaga aagcgcgact gatagacgcg ccagttcttg atcgagggcg acggcgacgg
     2761 tgtaacgagc ttgaccgcgg tggccgaata gctcacgcga gcgaacaacg catgcaatgc
     2821 ggcttcgtcg aagtcgtaac gcgcggacat gtcgttgatg aacgcgtcga cgttcgggtt
     2881 attcgcatag cgctgcggaa tgatctcttc ctcgaaggtc tgcccttgcg gcaccgccgg
     2941 ttgcggctgg ctttgcgcga gcagcacggg cggcttcttc gcggtgcttt gcgcgagcgc
     3001 cggactagtt gccatgaaca tgcacgatgc gacggacagt gcggtggcca cggtcttcag
     3061 gtgtagccgg tttcgtgctg acagagcaag cttgacggtc atagtgagct gggggcgcag
     3121 tgcgaaagag acagggggaa aacggttcgg ggcagtatac ccgacgcttt ctgcaaaacc
     3181 gtgcctggta cgtcacagtc gcatcgtgtg gtaacttggc aaatgttggc gcgcccagtg
     3241 agcgcgccct tggacggaga cacgccgcgc gcgcctcgaa ggtgccgcgc cgcgcaagag
     3301 gatcatggca acaggctttt attcccacgc cgactgtttg ctgcacgata tggggcaatg
     3361 gcatcccgaa tgcccggcgc gcctgcaggc aatcgaagat caattgatcg ctagccgcat
     3421 cgattcgctg atcgagcgcg agtcgccgcc gctcgccgac gaagccgcgc tgttgcgcgt
     3481 gcatacgcag gcccacgtcg attacattcg cagccgttcg cccgcggagg gtctcgccga
     3541 aatcgatccc gacacgtcga tgaacccgta caccttgcag gccgcgttgc gcgcggccgg
     3601 cgccgcggtg gccgcgaccg acgccgtgat cgaaggccgc tacgacaacg cgttctgcag
     3661 cgtgcgcccg ccaggccacc atgcggagcc ggcgcgtgca atgggcttct gcttcttcaa
     3721 caacgtcgcg atcgccgccc gtcatgcgct ggaagtgcac ggcatgcagc gcgtggcgat
     3781 catcgacttc gacgtccatc acggcaacgg caccgaagcg gccttttcgg gcgactcgcg
     3841 cgtgctgatg tgcagcatct tccagcatcc gttctatccg ttcaccggcg ccgacaatca
     3901 ggcgccgaac atgtgcaacg tgccgatgcc ggcgcgctcg aaaggcatgg cggtgcgcga
     3961 ggcggtcgac ctgttgtggc tgccgcgcct gcacgacttc aagcccgaga tgatcttcat
     4021 ttcggctggc ttcgacgcgc atcgcgagga cgatctcggc aacatgggtc tcgtcgaaga
     4081 cgactacgca tggatcaccg accaggtgcg cgagatcgcg aagcgttatg cggatgggcg
     4141 gatcgtcagt tgtctcgaag gcgggtacaa cctgtcggcg ctcgggcgca gcgtggtcgc
     4201 gcacgtgcgc tcactggccg gaatctgagc ggccgcgccg ccgcaagcta caggaactgc
     4261 gcgaacgtcg cgaaccgcat ccactcaacg aggaacgagc catgaatgcc gaggtgcgca
     4321 gtgcttcgct ggtcgaaacc gaacacgacg cctatggcgt gccgggcgtc gtccgcttga
     4381 cgatgaaccg gcctgatgcc ttcaactcac tctccgaagc cctgctggac gagttgcaag
     4441 cgacgctgac ggcgctggcg cagtctagtg cgcgggtggt ggtgatcgcc ggcgcgggcc
     4501 gcgcgttttg cgcgggacac gatctgaagg agatgcgcgc ggcgccgtcg ctggcttact
     4561 accaggcgtt gttcgcgcgc tgcacgaaac tgatgctgac gatccagcgc ttgccgcaac
     4621 cggtgatcgc gcgggtgcac ggcatcgcca cagccgccgg ctgtcagctg gtcgcgatgt
     4681 gcgatctggc agtcgccgcc gataccgcgc gctttgccgt gtcgggcgtc aacctgggtc
     4741 ttttctgcgc gacgccttcg gtgccgctgt cgcgcaatct gtcgcgtaag gcagcgctcg
     4801 aaatgctgct cactggcgat ttcatcgacg ccgtgcaagc gaaacaccaa ggcctcgtga
     4861 atcgcgtggt tccggccgac gcgctcgaca ccgagctcgc gcggctcgcg aaaagcatct
     4921 gcgcgaaacc cgtcgaagcg gtgcgcgccg gcaagggcct gttctatcgg cagttggaaa
     4981 tgggcattga agcggcgtat cagctggccg gccagaccat ggcctgcaac atgatggacg
     5041 agtcggcgct cgaaggcgtg caggccttca tcgacaaacg cccgcccgac tggaagcgct
     5101 agcgccgggt tgagccgttc ttgcagggag gcgcccccgc tctcagtcat ccggatgcct
     5161 gaacttctag atgcttcccg ctgctgaaat ctggcgggca tccgcgcgcc gacctgccca
     5221 gacggtttgt ttatgtcgaa acgagccgtc caggcctcaa gctctgggca gccgtttcac
     5281 gatccagtcg gtcagcgcgc caatcacccg atcccggtcc agatcgttca tcgtctcgtg
     5341 atagctgcct tcgtgcaggg tgagcgtctt gtccggcgag ccggcatgcg cgccgaaatc
     5401 gcggctgcct tcgggttcgg tgagcttgtc ggcggtgccg tgatacacca gcagcggcac
     5461 acgtagtccc gcacggccgc gctcgatgcg cgccatcgcc cgcaacaatt ccgcaccggt
     5521 acgcgccgga atcgcactgt gatgaaccag cggatcgttg cgattggcgt tcaccacggg
     5581 ctgaagacgc gacagcaagc cggcgtcgat tttcatcgcc gggaaggtcg gccagatgcg
     5641 gctgattatc tggctcgcct tgagcatcca gcgcggcaca tcgcggcccg gcgcgagcgc
     5701 ggggctcgac aggatcaggc cgctcagccg acgcccgctt gcctcgagcc gttcgattgc
     5761 ataaagcgcc gcgaccgcgc cacccatgct gtggcccatc agaaacagcg gcgcgcaact
     5821 gatcgcggct tcatcgagca aggcctgggc atcgagcaga tagtcgtcga agcggttgac
     5881 ccatacgcgc ctacccggcg catgcccatg tccgcgcaaa tcgatcgcga cgagttcaat
     5941 gccggccggg ttcagccggt ccgccagcgc cgcgtaacgc ccggcatgct cagcgagccc
     6001 gtgtaccagc gcgaccgtgg cgcgcagttg agtggttgcg ggccaccgat acaacggcag
     6061 ttcgatgccg tcgctcgtcg tgatcgtcga gcgcagcgcg gcgccgcctt tcggggcgcc
     6121 tgcaggggcg cccgctgcgg gacgcacatt gccgggtcga gtcgtttcca tagctgctgt
     6181 ttatcctgaa tgggtcctga ttctggttcg ccgatcatag tcgcagcgga cgcgccacgc
     6241 gcgagcctct cgcacctggc aggcctctaa tgccctttgg ttatgaaaag atcgtaattg
     6301 attattaaag ggaatgacga cgctgcggta gaattgcggc ctcaaatcct aataaaagcc
     6361 acggcggccg cttgtcggga gcgcacaacg ctcgttggca gactccgccg tttcgttttt
     6421 ccatgatcga aatacgcaat atctctcagc ggttcgccgg gccgcggggc tgggtcgagg
     6481 cgctgcacaa cgtcaatctg tcgattccgg ccggtgaggt gttcggcatt atcggccgca
     6541 gcggcgcggg caagagtacg ctcgtgcgca ccatcaacct gctgacgaga ccgagcgagg
     6601 gcaacatcgt tgtcaacggc cgcgatctga cgacgctgcc tgccgcgcaa ttgcgcgagg
     6661 cgcgtcgcga aatcggcatg atcttccagc acttcaacct gctgtcgtcg cgtacggtgt
     6721 acgagaacgt ggcgttgccg ctcgaactcg ccgggatgaa gcgcgacgag atcgaggcga
     6781 atgtgttgcc gctgctcgaa ctggtgggcc tgtcggcgca gaaggatcgt tatccggcgc
     6841 agatcagcgg cggacagaag cagcgcgtcg gcattgcacg tgcgctggcg agcaagccga
     6901 aagtgttgct ctccgacgaa gcgacttccg cgctcgatcc cgaaaccacc cgctcgatcc
     6961 tcgacctcct caagcgcatc aatcgtgaac tgaacctgac gatcgtgctg atcacgcacc
     7021 agatggacgt gatcaagcag gtctgcgacc gcgtcgcggt gctggatgcc ggtcgtgtgg
     7081 ttgaagaggg caaggtcatc gacgtgttcc tgcaaccgca tcatgaagtg acgcgcgcgc
     7141 tgattggcga cgtgatcgcg caggaactgc cgcccgccat gaaggcgcgc gtggctgaac
     7201 gcctgaagac cggcagcggc cacttgctgc gcctggcgtt cacgggctcg ggtgtcgatc
     7261 agccgatcct gtcggaaacg attcgccgct atgaactcga cttcaacatc ctgcacggcc
     7321 agatcgacga gatccagggg caggcgttcg gctcgctcgc ggtgctcgcg ggcggcgaac
     7381 cggcgaacgt ggcgcaggcg ctgacgtatc tgcgcgaaca gggtgtggtg gtggaggagc
     7441 tgtcgtatgt tgagtg
//
LOCUS       DATASH010000354         1672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2029242,
            whole genome shotgun sequence.
ACCESSION   DATASH010000354 DATASH010000000
VERSION     DATASH010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2029242"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /gene="dgt"
                     /locus_tag="VHV99_09950"
     CDS             complement(<1..525)
                     /gene="dgt"
                     /locus_tag="VHV99_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006052247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HEX3635796.1"
                     /translation="MSGVPAQEVLEAHLAPYAAHSAQSRGRRYPEAAPSARTEFQRDR
                     DRIVHSTAFRRLEYKTQVFVNHEGDLFRTRLTHSLEVAQIARSVARNLRVNEDLVEAI
                     SLAHDLGHTPFGHAGQDALNECMREHGGFEHNLQSLAVVDDLEEHYGAFNGLNLCFET
                     REGILKHCSRENARR"
     gene            complement(658..>1672)
                     /gene="aroB"
                     /locus_tag="VHV99_09955"
     CDS             complement(658..>1672)
                     /gene="aroB"
                     /locus_tag="VHV99_09955"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEX3635797.1"
                     /translation="IGQTALFAPHIAGSSVTIVTNTTVEPLYGDQLRAALAPLGKQVS
                     TVVLPDGEAYKNLETLNLIFDALLGSRADRKTTLIALGGGVIGDMTGFAAACYMRGVP
                     FIQVPTTLLSQVDSSVGGKTGINHPLGKNMIGAFYQPQAVIADIGALRTLPARELAAG
                     VAEVIKTGAIADAGFFNWIEANIEALNRCEPQALAEAVKRSCEIKASVVAQDEREGGL
                     RAILNFGHTFGHAIEAGLGYGEWLHGEAVGCGMVMAADLSVRMGYLDEAARKRLVDVI
                     VAAHLPTRAPALGDSRYVELMRVDKKAEAGAIKFILLKRFGETLITQAPDAEVHATLA
                     AAV"
BASE COUNT          279 a          533 c          548 g          312 t
ORIGIN      
        1 ccggcgcgcg ttctcgcgcg agcagtgctt gagaatgccc tcgcgcgttt caaagcaaag
       61 gttcagcccg ttgaacgcgc cgtagtgctc ttccagatcg tcgaccaccg cgaggctttg
      121 caggttgtgc tcgaagccgc catgttcgcg catgcattcg ttcagcgcgt cctggcccgc
      181 gtgaccgaac ggcgtatggc cgagatcgtg cgccagcgaa atggcttcga cgaggtcttc
      241 gttgacgcgc agattgcggg ccaccgaacg ggcaatctgc gcgacttcaa ggctatgggt
      301 gagccgcgtg cgaaacagat cgccctcgtg attgacgaat acctgcgttt tgtattcaag
      361 ccggcggaac gctgtggaat ggacgatacg gtcgcgatcg cgctggaatt cggtgcgcgc
      421 gctgggcgcg gcttcgggat agcggcgccc gcgtgattgg gcggaatgtg ccgcgtaggg
      481 cgcgagatgc gcttcgagta cttcctgcgc gggcacgccg ctcacgggcg tgccgggcgt
      541 gtctgcgggg ccatgtttta catcgtcgct gcgcctgtca gtcaccggat tctccgaaac
      601 atgggcggcg cctcgcgaca ctggtgcgtg acgctttgcc gcaatgccct gccgtttcta
      661 gacggcagcg gccagcgtgg catgcacctc ggcgtcgggc gcctgggtga tcagcgtttc
      721 accgaaacgc ttcagcagaa tgaatttgat cgcgccggct tccgccttct tgtcgacgcg
      781 catgagttcg acgtaccgcg agtcaccgag tgccggcgca cgcgtaggca gatgggcggc
      841 gacgatcacg tcgaccagac gcttgcgcgc ggcttcgtcg agataaccca tgcgcaccga
      901 caggtccgcc gccattacca ttccgcagcc gaccgcctcg ccgtgcagcc actcgccgta
      961 gccgagtccg gcttcgatcg cgtggccgaa ggtgtggccg aaattgagaa tcgcgcgcag
     1021 cccgccttcg cgctcgtcct gcgccacgac cgacgccttg atctcgcagg agcgcttgac
     1081 cgcctcggcc agcgcttgcg gctcgcagcg attgagcgcc tcgatgttcg cttcgatcca
     1141 gttgaagaaa ccggcgtcgg caatggcgcc ggtcttgatc acttcggcaa cgcctgctgc
     1201 cagttcgcgc gccggcagcg tacgcagcgc gccgatgtcg gcgatcacgg cctgtggctg
     1261 atagaacgcg ccgatcatgt tcttaccgag cggatggttg ataccggtct tgccgcccac
     1321 tgacgaatcg acttgcgaca gcaaggtggt cggcacctgg atgaacggca cgccgcgcat
     1381 gtagcaagcc gcggcaaaac ccgtcatgtc gccgatcacg ccgccgccga gagcgatcag
     1441 cgtagtcttg cgatcggcac gcgagcccag cagcgcgtcg aaaatcagat tcagcgtttc
     1501 gaggttcttg tacgcttcgc cgtccggcaa gacgaccgtg gagacctgct tgccgagcgg
     1561 cgcaagcgcc gcgcgcagtt ggtcgccgta aagcggctcg acggtggtgt tcgtgacgat
     1621 cgtgaccgaa ctgccggcga tatgcggcgc gaacaacgcg gtctggccga tc
//
LOCUS       DATASH010000355        17986 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5985064,
            whole genome shotgun sequence.
ACCESSION   DATASH010000355 DATASH010000000
VERSION     DATASH010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 17986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17986
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5985064"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..112
                     /locus_tag="VHV99_09960"
     CDS             <1..112
                     /locus_tag="VHV99_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018432023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="squalene synthase HpnD"
                     /protein_id="HEX3635798.1"
                     /translation="LALALLDEIERDGYHVLHQRIALTPIRKLWIAWRAR"
     gene            complement(166..1422)
                     /locus_tag="VHV99_09965"
     CDS             complement(166..1422)
                     /locus_tag="VHV99_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX3635799.1"
                     /translation="MNRRSFLSMGAAAGSTLWLPRVFGAQAAEATPAAELAQAAAALG
                     SPTNRGYGNLLILVELKGGNDGLNTVIPFADPTYYQLRKNIGIKREQAIQLDERTALH
                     PSLQPLMPLWQSRQLAIVQGVSYVQPNLSHFRSIEIWDTASRSDKYLREGWLTRAFAQ
                     APVPAGFAADGVVIGSAEMGPLANGARAIALVNPAQFVKASRLVTPVSLHERNPELAH
                     ILDVENDIVKAADRLRPTQGQAQLKTVFPGGAFGGSIKTAMQVLAAGNTPQGQPKSGQ
                     GVAVIRLTLNGFDTHQNQPGQQAALLKQLADGFASMKSALVELGRWNETLVMTYAEFG
                     RRPRENQSNGTDHGTVAPHFVMGGRVRGGLYGVPPVLARLDGNGNLPVGVDFQQLYAT
                     VLGPWWGLDASAILQQRFEPLPLLRV"
     gene            complement(1542..2183)
                     /locus_tag="VHV99_09970"
                     /pseudo
     CDS             complement(1542..2183)
                     /locus_tag="VHV99_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009980659.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
     gene            complement(2463..2789)
                     /locus_tag="VHV99_09975"
     CDS             complement(2463..2789)
                     /locus_tag="VHV99_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635800.1"
                     /translation="MKGGVWPNVRLSVIALAVAGTLAGTLGPTLAHAQPAAGRAASEH
                     ALRSANPKANRRSPNNDSPASDIMLRTAVPPDLDQRRRDGHMTPDERRLLRQHIEDAV
                     RELYKR"
     gene            3028..3753
                     /gene="ompR"
                     /locus_tag="VHV99_09980"
     CDS             3028..3753
                     /gene="ompR"
                     /locus_tag="VHV99_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HEX3635801.1"
                     /translation="METKNPSKILVVDDDPRLRDLLRRYLGEQGFNVYVAENAPSMNK
                     LWVRERFDLLVLDLMLPGEDGLSICRRLRGSNDRTPIIMLTAKGEDVDRIVGLEMGAD
                     DYLPKPFNPRELVARIHAVLRRQSPSELPGAPSETTEVFEFGEFALNLATRTLTKAGQ
                     EIPLTTGEFSVLKVFARHPRSPLSREKLMELARGREYEVFDRSLDVQISRLRKLIEPD
                     PGSPRFIQTVWGLGYVFIPDGAA"
     gene            3971..5320
                     /locus_tag="VHV99_09985"
     CDS             3971..5320
                     /locus_tag="VHV99_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3635802.1"
                     /translation="MRIDRRLLTLAFGGLFWRTFLLIALLIAVSLAAWFQSFRVIERE
                     PRAQRVALQLVAIVKLTRTALLYSDPDLRRALLQDLESNEGVRVYPRETTDKYKLQPD
                     ESLNRLIEHDIRGRLGDDTVIAQSVNDIPGVWISFKIDDDDYWVALDRDQLDNATGLQ
                     WAGWGVFALALSLFGAAFITSLVNRPFARLAMAARKVGSGQSPEPLPERGMGVAAETN
                     RSFNQMVQDLEQLEADRALMLAGISHDLRTPLARLRLETEMSPSDQATKDAMVDDIEQ
                     MDMIIGRFLDYARPVQRVPEPVDLSVIAGELAARMQSEDSMRLITRLAPSAVIEADET
                     DMRRVVGNLLENARKYGLSDGDGIPHVILETRVSHSRVELSVVDEGPGIPEDQLALVT
                     RPFYRVNSARTQANGTGLGMAIVLRLVGRYRGALRLRNRTPGPGLEVTIEFPLAKGA"
     gene            5510..6058
                     /locus_tag="VHV99_09990"
     CDS             5510..6058
                     /locus_tag="VHV99_09990"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX3635803.1"
                     /translation="MKTVGDKVEAFTVTAAKPGFNNHEENGVSAFEEITEQSFPGKWK
                     IIYFYPKDFTFVCPTEIVEFGKLAKAFEERDAVLMGGSVDNEFVKLAWRREHKDLSKL
                     NHYSFGDVKGELIDQLGVRDAEEGVALRATFIVDPDNTIQHVSVNNLNVGRNPEEVLR
                     ILDGLQTDEECPCNRAVGGSTL"
     gene            complement(6114..6290)
                     /locus_tag="VHV99_09995"
     CDS             complement(6114..6290)
                     /locus_tag="VHV99_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635804.1"
                     /translation="MRKQIIRIAQATTSRKHQQAKPEKTKKIKTPKTLTNTGAQQKTS
                     IRLSRETTTSPQHQ"
     gene            complement(6363..6842)
                     /gene="ispF"
                     /locus_tag="VHV99_10000"
     CDS             complement(6363..6842)
                     /gene="ispF"
                     /locus_tag="VHV99_10000"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEX3635805.1"
                     /translation="MDFRIGQGYDVHALVPGRPLIIGGVTIPYERGLLGHSDADVLLH
                     AITDAIFGAAAMGDIGRHFSDTDAAFAGADSRVLLRECLARVKAAGFSIANVDSSVVA
                     QAPKLAPHIEGMRANIAADLDLPVERVNVKAKTNEKLGYLGRGEGIEAQAVVLLIKS"
     gene            complement(6899..7615)
                     /gene="ispD"
                     /locus_tag="VHV99_10005"
     CDS             complement(6899..7615)
                     /gene="ispD"
                     /locus_tag="VHV99_10005"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEX3635806.1"
                     /translation="MTSRLFALIPCAGTGSRSGAAMPKQYRTVAGRDMLHYSLAAFDA
                     CSEFAQTLVVIAPDDQHFDARRFSGLRFAVRRSGGASRQASVLNGLHALAEFGAHDDD
                     WVLVHDAARPGITPSLIRTLTGALKDDAVGGIMALPVADTLKRIDAGSSDGRIARTEA
                     RDGLWQAQTPQMFRIGMLRTAILRAQADGHDLTDEASAIEWMGHAPKLVQGSLRNFKV
                     TYPEDFDLVEAILSRPSSPS"
     gene            7737..11219
                     /gene="mfd"
                     /locus_tag="VHV99_10010"
     CDS             7737..11219
                     /gene="mfd"
                     /locus_tag="VHV99_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX3635807.1"
                     /translation="MPDIAATSQYSPPVALVKAGQRFAFDGTHGSSDALLIARYHLAY
                     REKVPLLAVVCESAVDAQRLAQEIGFFAPEARVRLLPDWETLPYDTFSPHQDLVSERL
                     ATLHDLGEGRCDILLVPATTALYRMPPASFMAGYTFSFSQGERLNEAKLKAQLTLAGY
                     EHVSQVVRPGEYCVRGSLLDLYPMGSALPYRIDLFDDQVDSIRAFDPDTQRSLYPVKD
                     VRLLPGREFPFDEAARTAFRSRWRETFEGDPSRASIYKDIGNGVPSAGIEYYLPLFFD
                     ETATLFHYLPEGAQLAFVGDLDAAIKRFTNDTKQRYNFLSHDRDRPILEPQRLFLSDE
                     DFFTFAKPFARLVLPANAGGGWSVPLPNLAIDRHAEDPVSALRAYLDTTPNRVLFAAE
                     SAGRRETLLQLLADNRLRPASSDSFQDWLVGDARFSLGVAPLANGFAVPADGFAIITE
                     TEIYGPLARRTGRRRQEQASNVDSMVRDLSELKVGDPVVHSQHGIGRYMGLVTMDLGE
                     GETEFLHLEYASNSKLYVPVAQLHVISRYSGADPESAPLHSLGSGQWEKAKRRAAQQI
                     RDTAAELLNLYARRALREGHAFALEPKDYVKFAESFGFEETPDQAAAIAAVIGDMTSG
                     KPMDRLVCGDVGFGKTEVALRAAFIAVMGGKQVALLSPTTLLAEQHTQTFTDRFSDWP
                     VRIAELSRFKSTRAVNAAIQQINEGTVDIVIGTHKLLSSDVQFKRLGLVIIDEEHRFG
                     VRQKEALKALRAEVDVLTLTATPIPRTLGMALEGLRDFSVIATAPQKRLAIKTFVRRE
                     EDSVIREAMLRELKRGGQVYFLHNEVETIENRRQMLEALVPEARIAVAHGQMHERELE
                     RVMRDFVAQRANVLLCTTIIETGIDVPSANTILIHRADKFGLAQLHQLRGRVGRSHHQ
                     AYSYLLVHDPQGLTKQAQRRLEAIQQMEELGSGFYLAMHDLEIRGTGEVLGDKQSGEI
                     HEIGFQLYTDMLNDAVKALKEGKEPDLTAPLAATTEINLHAPAILPADYCGDVQERLS
                     LYKRLANCEHNDSIDSIQEELIDRFGKLPPQAHALVETHRLRLAAKPLGISKIDAGET
                     VIGLQFIPNPPIDAMRIIEMVQKHKHIKLAGQDKLRIETRSPDLAVRVATVKETLRAL
                     GTPSRGTAAAAAVR"
     gene            11417..12667
                     /gene="argE"
                     /locus_tag="VHV99_10015"
     CDS             11417..12667
                     /gene="argE"
                     /locus_tag="VHV99_10015"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008777 - acetylornithine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HEX3635808.1"
                     /translation="MSHVAESAQSSPTPASESNASSPATLPASLPWVTRLVSMDTVSR
                     NPNLGLIETVRDELRAVGIEATLTHDQSGKWANLFATIPAHDGETNGGVVLSGHTDVV
                     PVDGQQWDSDPFKPEIRGDKLYGRGTCDMKGFIGAALSLVPDMQRTKLARPIHFALSF
                     DEEVGCAGAPLLITDLMKRGVKPDGCIVGEPTSMRPIVAHKGINAYQCCVRGQAAHSS
                     LTPKGLNAIEYAARLICYIRDMADQFRAQGPFDELYDVPFTTAQTSTIVGGNAINTVP
                     AECKFQFEFRNLPTLDPEPIFARIDQYARETLLPKMLREHPSAAIEITKIAAAPGLDS
                     SEQAAITQLVRALTADQDKRKVAYGTEAGLFSLAGIPSIVCGPGDIQQAHKANEFVAL
                     DQLVACERFLQKFIHSMSVEAHVH"
     gene            12724..13758
                     /locus_tag="VHV99_10020"
     CDS             12724..13758
                     /locus_tag="VHV99_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX3635809.1"
                     /translation="MSTATPQHTDHTIDGEPIPTLDAIATQHFALTPWVARTPVFDRL
                     DFPSLEGTVVNFKFELLQAGGSFKARGAFTNLLALDEAQRSAGVTCVSGGNHAVAVAY
                     AAMRLGISAKVVLFRAANPARVALCRQYHADIVFAEDIAEAFELVRGIEAEEGRYFVH
                     PFNGYRTVLGSATLGYEWATQTPDLEAVIVPIGGGGLAAGVATAMRLANPRVHVYGVE
                     PEGADAMGKSFAANHTVKMGHMHGIADSLMSPHTEEYSYELCRRHIDQLVTVSDDQLR
                     AAMLTLFGQLKLAVEPACAAATAALLGPLRESLQGKRVGVLLCGTNTDPVSFAAHIER
                     ARHSQSQFPE"
     gene            13858..14307
                     /gene="mscL"
                     /locus_tag="VHV99_10025"
     CDS             13858..14307
                     /gene="mscL"
                     /locus_tag="VHV99_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEX3635810.1"
                     /translation="MSMIKEFKEFALKGNVMDLAVGVIIGGAFSTIVNSIVKDLIMPV
                     VGVATGGLDFSNKFVRLGDIPPSFKGSPESYKDLQTAGVAVFGYGSFITVAINFLILA
                     FIIFLMVKFINNLRKPLEAAPAEPPPPPEDVLLLREIRDSLKNAPPR"
     gene            complement(14476..16449)
                     /locus_tag="VHV99_10030"
     CDS             complement(14476..16449)
                     /locus_tag="VHV99_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018432035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3635811.1"
                     /translation="MTQEVPTQSAAYVLVVDDDEGILRLARKSLERAGCRVAICPGVE
                     AARERLATGAPDLLVLDYQLSGAESGLDFFRRLRAEGVRIPAILVTGFTDESRVIEAL
                     RAGVSDVVPKSGDYLDYLPEAVERVLSQVRLQKASDEALLLRDREQHYRTLSEALPHL
                     VLTCNAAGDCDFLSKQWFDYTGLTENRSYGLAWLEAVHPDDREEIRRTWLKAVTSRAG
                     DYRHELRIRRHDGEYRWFDARAISTRDAEGNVSKWFGSCTDIHPQREAIEERERLLAS
                     EQAARQIAEEANRAKDRFLAMLSHELRTPLTPVLAGASVLEMIPGLPDQARASVRMIR
                     RNVELEARLIDDLLDLTRVANGKLRLSLETVDVHEVMDSVLELFRSEIQVKQQDVHVC
                     KNAHNHYVLADRARLQQMLWNLIRNAAKFTPDGGHIYVRTRDERMHVQISVEDTGIGI
                     EPEQIGKLFNAFEQGNQNMTRQFGGLGLGLAITKALTDVHGGTVIAQSPGAHCGATFT
                     ITLPTAAAPVESTPVATPDEVHPPGLLTILLIEDHVDTAEVMEQLIRSLGHDVTTVGR
                     VDDALAATQSQTFDLVISDVGLPDGTGLDFVKAFREHSDAPAVALTGFGTDEDVRRCL
                     SAGFTSHLTKPVNFGQLETMIESAVSLRVEKNA"
     gene            complement(16527..17006)
                     /locus_tag="VHV99_10035"
     CDS             complement(16527..17006)
                     /locus_tag="VHV99_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3635812.1"
                     /translation="MSHGETVSIVLIEDDDGHATLVERNLRRAGISNGFVRFRDGQQA
                     LDYFFGPVPADPAASPYPPREDLTNFVVLLDLKMPRVDGFEVLRRLKDSPQTAAVPVI
                     VLTTTDDPREIARCYELGCNVYITKPVEYEAFIEAVRRLGFFLQVVKLPPGHRLTAP"
     gene            complement(17135..>17986)
                     /locus_tag="VHV99_10040"
     CDS             complement(17135..>17986)
                     /locus_tag="VHV99_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3635813.1"
                     /translation="QAMLKTRLEARARELAGVNEELRQETQDNEMFIYSVSHDLRSPL
                     VNLQGFSRELQVSCDELDSIVAAAKLPEAEHRRMVHILDGDVRESLQYLRSAVTQAAS
                     IIDALLRISRAGRLEYQWQRVSVGRVVGRVVDALQSAIGQRRAVITVRELPPAWGDPG
                     AIEQIFSNLIGNALNYLDPARHGRIEIGALEPELEAVDEREPRALRTRTYYVRDNGLG
                     IPAAYMSKVFRAFQRLHGDVADGDGIGLAVVRRMVERHGGRVWVESAEGAGSTFFVVL
                     PEQPARH"
BASE COUNT         3477 a         5746 c         5318 g         3445 t
ORIGIN      
        1 gctggcgttg gcgctgctcg atgaaatcga acgcgacggc tatcacgtgc tgcatcagcg
       61 cattgcgttg acgccgatcc gcaaactctg gatcgcgtgg cgcgcgaggt aggcgagcga
      121 cacaacggcc gttggctcat gctgatgagg caacgctgtc catcgtcaga cgcgtagcaa
      181 cggcagcggt tcgaaccgct gctgcaggat cgcggacgcg tcgagtcccc accacggccc
      241 gagcacggtc gcgtagagtt gctggaaatc gacgccgacc ggcaagttgc cattgccgtc
      301 gagccgcgcg agcaccggcg gcacgccgta caaaccgccc cgcactcgcc cgcccatgac
      361 gaaatgcggc gccacggtgc cgtggtctgt gccgttgctc tgattctcac gcggccgcct
      421 gccaaactcg gcataggtca tcaccagcgt ctcgttccag cggccgagtt ccaccagcgc
      481 cgatttcatc gacgcaaacc catcggccag ttgtttgagc agcgccgcct gttgacccgg
      541 ctgattctgg tgcgtatcga aaccgttcag cgtcaaacgg atcaccgcga caccctgtcc
      601 gctcttcggc tgtccttgcg gcgtattccc agccgcgagc acctgcatcg ccgtcttgat
      661 cgaaccgccg aatgcgccgc cgggaaacac cgtcttcaat tgcgcctgac cttgcgtggg
      721 acgcagacga tcggcggctt tcacgatatc gttctcgacg tcgagaatgt gcgccaattc
      781 cggattgcgc tcgtgcagcg acaccggcgt gacgaggcgc gaagccttga cgaactgtgc
      841 cggattgacc agcgcgatcg cgcgtgcgcc gttggcgagc ggtcccattt cggcgctgcc
      901 gatcaccacg ccgtcggcgg caaaaccagc gggcaccggc gcctgcgcga atgcccgcgt
      961 gagccagccc tcgcgcagat atttgtccga ccgcgacgcg gtatcccaga tctcgatcga
     1021 gcggaaatgc gacaggttcg gttgcacata gctgacgccc tgcacgatcg ccagttgccg
     1081 gctttgccaa agcggcatca gcggctgcaa cgacggatgc agcgcggtgc gctcgtcgag
     1141 ttgaatcgcc tgctcgcgtt tgatgccgat gtttttgcgc agctgatagt aagtgggatc
     1201 ggcaaacgga atcacggtat tcaaaccatc gttgccgccc ttcagttcga cgagaatcag
     1261 caggttgcca tagccgcgat tcgttgggct gcccagggct gccgccgctt gtgccaattc
     1321 tgctgctggt gtcgcttctg ccgcttgcgc gccgaacacg cgtggcagcc agagtgtcga
     1381 gccggcggct gcgcccatcg agagaaagct gcgtcggttc atcctgcacc tcgttcgatt
     1441 gatatggcgc atgtcctgtt gacgtcgacg gaccgttcat ttccgctgct catcgtcgcc
     1501 agacagttga gcggttcaat tccgcccccg gccccggtag ttcacttcaa ctgataagcc
     1561 ggatccatca acaacgcatc cagataagcg ctggcggtcg aaccggtgtc gatcgcgtcg
     1621 accggcatga gcggcaagac cgcgtgctgc aactggaact cggccgaaac ccgggcttcg
     1681 tcgtcggcgc ggtgttgtac tgcgcaagcc atgtgccgat gtcaaagcgt acgccgcctt
     1741 ggcctgcctg gccgatgcgc gccgttgcgc gttgcaacgg agatttcggc tgcctgttat
     1801 ctgctacgtc gctcgccgcg atctttgcat tcgtcggagc gatcatgcgt tgcggaccgg
     1861 cggcctcagt cgcgcgaaac agttgctcga cgaattgctt gcgcgctagc agcgtcgaac
     1921 tattgatcca tatcgtaccg cccggccacc cttttgacgt tcggcgggta gagcaggttc
     1981 tcgccgagcg tgcggatccg cgccgcgaac ggcgccgggt tgtcgtagcc gatatcgaag
     2041 gcacgcaatg tcccgacaac gaactcaacc ggcgatttca caaggacgcc gcgatcgtct
     2101 tcgctccaga acgcgtcgct catgaagagg ccgcgcagcg ccaccttgat atcgtagtga
     2161 ctcgcgcgga actgcgcggc gatcggtacg ttgacgccgg aacacaccag ccagtcacga
     2221 ccgcgagcgc ggcggcggtg actgcaagcc gtcggctgct accgtgaccg cctgtcagcg
     2281 atttcatcat cgatcatccg gttgcaaggc gtcggcaaaa ggcatttcgc gcatgcaatc
     2341 gcgctgcgct ctggcgactc aacgggcaga tggaacggca aagtcgacag ggaacccgca
     2401 acgaaaaatc tttcacgcac gcaaaagcgc gcttggcgtg cccggcccag gcacgaaaat
     2461 agctaccgct tatagagttc ccggacagcg tcttcgatgt gctgccgtaa cagacgccgt
     2521 tcgtcgggcg tcatatgacc atcacgacga cgttgatcaa gatcgggagg gactgcggtg
     2581 cgcaacatga tgtctgacgc ggggctgtcg ttgttggggc tgcggcgatt cgccttggga
     2641 ttggcggacc tgagtgcgtg ctcgctcgcc gctctcccgg ccgcaggctg ggcgtgggcg
     2701 agcgttggtc ctagtgtgcc tgcaagcgtc ccggctaccg ccagtgcaat tacgcttagg
     2761 cgcacattcg gccaaacccc tcccttcatg ttgttccctt cgtggcgcgg caaccggcta
     2821 cctcaaatgc gtatacagac cccggtagcg agccgggtgc gagagtttta gtcgtgtgaa
     2881 gtatccaccg aacagccgca ttcagtaaag gagtctacct gtccctgctt tacgtcgtgc
     2941 cacagtgcgg gtaaattttg taactgactg taacccgcta aatgatgcaa ccgcgcgccc
     3001 ctttctaatc gcgctaagat gccgaccatg gaaaccaaaa acccttcgaa aatcctcgtc
     3061 gtcgacgacg atccgcgcct gcgcgatctg ctgcgccgct accttggtga acagggcttc
     3121 aatgtctacg ttgccgagaa cgcgccgtcc atgaacaagc tctgggtgcg tgagcgcttc
     3181 gatctgctcg tgctcgatct gatgctgcct ggtgaagacg gcctgtcgat ctgccgccgc
     3241 ttgcgcggca gcaacgaccg tacgccgatc atcatgctga cagccaaggg tgaagacgtt
     3301 gatcgtatcg tcgggctcga aatgggcgcc gacgactatc tgcccaaacc gttcaatccg
     3361 cgcgaactgg tcgcgcggat tcatgcggtg ctgcgccgcc agtcgccctc ggagttgccg
     3421 ggcgcgccgt ccgaaaccac cgaagtattc gagttcggcg agttcgcgct gaatctcgcc
     3481 acgcgcacgc tcaccaaggc cggccaggaa attcctctga ctacgggtga gttctcggtg
     3541 ttgaaggtat ttgcgcgcca cccgcgttcg ccgctgtcgc gtgaaaagct gatggagctt
     3601 gcgcgcggtc gtgaatacga agtgttcgac cgcagtctcg acgtgcagat ttcgcgtctg
     3661 cgcaagctga tcgaacctga tccaggcagc ccacgtttca ttcagaccgt ttggggtctg
     3721 ggctacgtgt tcattcctga cggcgcagcc tgagattgcc cccatttact gtgtcgacca
     3781 gagttggcgc tttagcgcct tacctctggt cccatgcaaa gcggtcgacc agagttggtg
     3841 ctttagcgcc ttatctctgg tcccatgcaa agcagtcgac cagagttggc gctttagctg
     3901 tcgaccagag ttcgcgcttc agcgcaatga ctctggtccc atgcaggata gtttccatta
     3961 agaagggccc atgcggatcg accggcgcct cctgacgctc gcgttcggcg gccttttctg
     4021 gcggaccttt ctgttgatcg cgctgctgat cgcagtcagt ctcgccgcgt ggttccagag
     4081 cttccgggtg atcgaacgcg agccgcgcgc acagcgcgtg gctttgcaac tggttgccat
     4141 cgtcaagctc acacgcactg cgctcctcta ttccgatccc gacctgcggc gcgccctgct
     4201 gcaggatctg gagagcaatg aaggggtgcg cgtgtaccca cgcgaaacca ccgacaagta
     4261 caaactccag cccgacgagt cgctgaaccg gctgatcgaa cacgacattc gcggccgcct
     4321 cggtgacgac acggtcattg cacagagcgt caacgacatc cccggcgtct ggatcagctt
     4381 caagatcgac gacgacgact actgggtagc gctcgaccgc gatcaactgg acaacgcgac
     4441 aggcttgcaa tgggccggct ggggcgtgtt cgcgctggcg ctatccctgt ttggcgcggc
     4501 gttcatcacg agtctcgtga accgcccgtt cgcccggctc gccatggccg cgcgcaaggt
     4561 gggctcggga caatcgcctg agcccttgcc tgaacgcggc atgggtgtcg ccgccgagac
     4621 caatcgcagt ttcaaccaga tggtgcagga cctggaacaa ctcgaggctg accgcgcgct
     4681 gatgctcgcg ggtatttcgc acgatctacg cacaccgctt gcacgcctgc ggctcgaaac
     4741 cgaaatgagc ccgtccgatc aggctaccaa agacgcgatg gtcgacgaca tcgagcagat
     4801 ggacatgatc atcggccggt tcctcgatta cgctcgtccg gtacagcgtg tgcctgagcc
     4861 tgtcgatctg tcggtgatcg ccggcgaatt ggccgcgcgg atgcagagcg aggacagcat
     4921 gcggctgatc acgcgtcttg cgccgtcggc ggtgatcgag gcagatgaaa ccgacatgcg
     4981 tcgcgtggtc ggcaatctgc tggagaacgc acgcaaatac ggcttgagcg atggcgacgg
     5041 tataccgcat gtgattctcg aaacgcgggt gtcgcattca cgggtcgagt tatccgttgt
     5101 cgacgaagga cccggaattc ctgaagacca actggccctt gtcacacgac cgttctaccg
     5161 tgtgaactcg gcgcgcaccc aggcgaacgg cacggggctg ggcatggcca ttgtgctgcg
     5221 actggtcggc cgttatcgcg gcgcgctgcg actgcgcaac cgcacgccgg gacctgggct
     5281 cgaagtgacg atcgagtttc cgctggcaaa aggcgcctga ttttccgcgc ctctgcacgg
     5341 tttctgcgac caccggcctc gtcgaatctc agcacgcgac ctgctgctat caaaccaaac
     5401 tggtggacca acgatctaat aggccaatat cgggaacgac taacgtgctc gttatggcga
     5461 gccggtagcc tcaacatgca atttttccat cagacgaagg agtattcgca tgaaaaccgt
     5521 tggcgataaa gtcgaagcgt tcaccgtcac tgctgccaag ccgggcttca ataaccacga
     5581 agagaacggc gtttccgcat tcgaagagat cacggaacag tcatttccgg gcaagtggaa
     5641 gattatctat ttctacccta aggatttcac gttcgtctgt ccgacggaaa tcgtcgaatt
     5701 cggcaaactg gccaaggctt tcgaagagcg cgatgcggtc ttgatgggcg gcagcgtgga
     5761 caacgaattc gtcaagctgg catggcgccg tgagcacaag gacctgagca agctgaacca
     5821 ttattcattc ggcgacgtga agggcgagct gatcgaccag ctgggcgtgc gcgacgcgga
     5881 agaaggcgtg gcgctacgtg cgacgttcat cgtcgatccg gacaacacga tccagcacgt
     5941 ttcggtgaac aacctgaacg tcggccgtaa tccggaagaa gtgctgcgta ttctggacgg
     6001 tttgcagacg gacgaagagt gcccttgcaa tcgcgcagtc ggcggctcaa cgctgtagga
     6061 agcgctgtcg gaagcccgta aagcttgaat gatggttgat gctgctgcgc aggctattga
     6121 tgctgagggg aagtagttgt ttcgcgcgat aggcggattg aggttttttg ctgcgcgcca
     6181 gtgtttgtga gtgtttttgg tgtttttatt tttttggtct tttctggttt ggcttgttgg
     6241 tgttttcttg atgtggttgc ttgtgcgatt cttattatct gttttctcat ggttttggcc
     6301 tttccttgaa ttgatattgg tctattagcg ttgcccctgc acggggcaac accaatagac
     6361 cactaactct tgatcaacaa aaccacagcc tgcgcctcaa tcccctcccc gcgcccgagg
     6421 taacccagct tctcattagt cttggcctta acattcaccc gctcgaccgg caggtcaaga
     6481 tccgcagcga tattagcccg catcccttca atatgcggcg ccagcttggg cgcctgcgcc
     6541 accacactgc tatccacatt agcaatagaa aacccagcag ccttaacccg agcaaggcac
     6601 tcccgcaaca aaacccggct atccgcgcca gcaaacgcag catcggtatc ggaaaaatgc
     6661 cgcccaatat cccccatcgc ggcagcgcca aaaatcgcat cggtaatcgc atgcaacagc
     6721 acatccgcat ccgaatgccc aagcagtccg cgctcataag gaatcgtcac gccgccaata
     6781 atcaacggac gtcccggcac cagcgcatgc acgtcataac cctgtccaat tctaaaatcc
     6841 atatgcgtca aaccctcgat cccattaact aaaatttgat tgatcccgat tggtccattc
     6901 aggaaggcga agatggccgg ctcagaatcg cctcgaccag atcgaagtct tccggatacg
     6961 taaccttgaa gttgcgcaaa ctgccctgaa ccaacttcgg cgcgtgtccc atccattcga
     7021 ttgcgcttgc ttcgtcagtc agatcgtgac cgtcagcctg cgcgcgcaaa atcgccgtgc
     7081 gcaacatgcc aatgcggaac atctgcggcg tctgcgcctg ccacagccca tcgcgcgctt
     7141 cggtgcgggc gattcgcccg tcggacgacc ccgcgtcgat ccgcttcaaa gtgtccgcca
     7201 ccggcaacgc catgatgccg cccaccgcgt cgtccttcag cgcaccggtc agcgtgcgga
     7261 tcaacgaagg cgtaatgccc ggccgcgccg cgtcatgcac cagcacccaa tcgtcgtcgt
     7321 gcgcgccgaa ttcggccagc gcgtgcagcc cgttcagcac cgacgcctgg cgcgacgccc
     7381 cgcccgagcg gcgcacggca aaacgcaagc cgctgaaacg gcgggcgtcg aagtgctggt
     7441 cgtcgggggc aatcacgaca agggtttgcg caaactcgct gcacgcatca aaagccgcca
     7501 gcgagtaatg caacatgtcg cgaccggcga cagtgcgata ttgtttgggc atcgcggcgc
     7561 cggaacggct gccggtgcct gcgcacggaa tcagggcaaa tagacgggaa gtcacgagtg
     7621 cggatgccgc gaggtcaaag taaaggacgg attttataat aggtccttcg catccacgcc
     7681 ccttcacgcg taaacttgcc gatcacgtgt gagcctgagg ccgctttttc tcttttatgc
     7741 cagacatcgc cgcaacatcg cagtactccc cgcccgtcgc gctcgtcaag gccggtcagc
     7801 gttttgcctt cgacggcacg cacggttcgt ccgacgcgct gctgatcgcc cgctaccatc
     7861 tcgcctatcg cgagaaggtg ccgctgctgg cggtcgtctg cgaaagcgcc gtcgacgcgc
     7921 agcgcctcgc gcaggaaatc ggctttttcg cgcccgaggc acgcgtgcgc ttgctgccgg
     7981 actgggaaac gctgccttac gatactttct cgccgcacca ggatctcgtc tccgagcggc
     8041 tcgccacgct gcacgacctc ggcgaaggcc gctgcgacat cctgctggtg cccgccacca
     8101 ccgcgctgta ccgcatgccg cccgcctcgt tcatggcggg ctacacgttc tccttctcgc
     8161 aaggcgagcg cctcaatgaa gccaaactga aagcgcagtt gacgctcgcc ggctacgaac
     8221 acgtaagcca ggtggtgcgt cccggcgaat actgcgtgcg cggctcgctg ctcgacctgt
     8281 atccaatggg ctcggcgctg ccgtaccgga tcgacctgtt cgacgaccag gtcgactcga
     8341 tccgcgcgtt cgaccctgac acgcagcgca gcctctatcc ggtgaaggac gtgcgtttgc
     8401 tgcccggccg cgaattcccc ttcgacgaag ccgcccgcac cgccttccgc agccgctggc
     8461 gcgagacctt cgagggtgat ccgagccgtg cgtcgattta caaggatatc ggcaacggcg
     8521 tgccatcggc gggcatcgaa tactatctac cgctcttctt cgacgaaacc gccacgctgt
     8581 tccactatct gcccgagggc gcgcaactgg cgttcgtcgg cgatctggac gcggcgatca
     8641 agcgcttcac caacgacacg aagcagcgct acaacttcct gtcgcacgac cgggaccggc
     8701 cgattctgga accgcagcgg ctgtttctct cggacgagga tttcttcacg ttcgccaagc
     8761 cgttcgcgcg gctcgtcttg cccgccaacg cgggcggcgg ctggtccgtg ccgctgccca
     8821 atctcgcgat cgaccgccat gcggaagatc cggtttcggc actgcgcgcc tatctcgaca
     8881 ccacgccgaa ccgcgtgctg ttcgccgctg aatcggccgg ccggcgcgaa accctgctgc
     8941 aattgctcgc cgacaatcgt ctgcggccgg cgtcgagcga cagcttccag gactggctcg
     9001 tcggcgacgc gcgtttttcg ctaggcgtcg cgccgttggc caacggcttt gccgtgccgg
     9061 ccgacggctt cgcgatcatc accgagaccg agatatacgg cccgctcgcg cgccgcacgg
     9121 ggcggcgccg ccaggaacag gccagcaacg tcgattcaat ggtgcgggat ctgtccgagc
     9181 tcaaggtcgg cgatccggtc gtgcattcgc agcatggcat cggccgctat atgggcctcg
     9241 tcacaatgga cctcggcgaa ggcgagaccg agttcctgca cctcgaatac gcgagcaaca
     9301 gcaagctcta cgtgccggtc gcgcagttgc atgtgatctc gcgctacagc ggcgcggatc
     9361 cggaaagcgc gccgttgcat tcgctcggct cgggccagtg ggaaaaggcc aaacgcagag
     9421 ccgctcaaca gatccgcgac accgctgcgg aactgctgaa cctgtacgcc cggcgcgccc
     9481 tgcgcgaagg ccatgcattc gcgctcgaac cgaaagacta tgtgaagttc gccgagagct
     9541 tcggcttcga ggaaactccg gaccaggccg ccgcgattgc cgcggtgatc ggcgacatga
     9601 caagcggcaa accgatggat cgcctcgtgt gcggcgacgt gggcttcggc aagaccgagg
     9661 tggccctgcg cgcggcattc attgcggtga tgggcggcaa gcaggttgcg ctgctctcgc
     9721 ccaccacgct gctcgccgaa cagcacacgc aaacgtttac cgaccgtttc tccgattggc
     9781 cggtgcgcat cgccgaactg tcgcgcttca agtcgaccag ggcagtcaac gcggcgatcc
     9841 agcagatcaa cgaaggcacg gtcgatatcg tgatcggcac gcacaagctg ttgtcctcgg
     9901 atgtgcagtt caagcggctg gggctcgtga tcatcgacga ggaacaccgc ttcggcgtgc
     9961 gccagaaaga ggcgttgaaa gcgttgcgcg ctgaagtcga tgtgctcacg ctcaccgcca
    10021 cgccgatccc gcgtacgctc ggcatggcgc tcgaaggcct gcgcgatttc tcggtgatcg
    10081 ccaccgcgcc gcaaaagcgg ctcgcgatca aaaccttcgt gcgccgcgaa gaagacagcg
    10141 tgattcgcga agccatgttg cgcgagttga agcgcggcgg ccaggtgtac tttctgcaca
    10201 acgaagtcga gacaatcgag aatcgcaggc agatgctcga agcgctcgta cctgaagcac
    10261 gtatcgcggt cgcgcacgga cagatgcatg agcgcgaact cgaacgcgtg atgcgcgatt
    10321 tcgttgcaca gcgcgcgaac gttctgctgt gcacgacgat catcgaaacc ggtatcgacg
    10381 tgccgagtgc caacacgatc ctgattcacc gcgccgacaa gttcggtctc gcgcaattgc
    10441 accagttgcg tggccgcgtc ggccgctcgc atcaccaggc gtactcctat ctgctggtgc
    10501 atgacccgca aggcctgacg aagcaggcgc agcggcgtct cgaagccatc cagcagatgg
    10561 aggaactcgg ttcgggcttc tatctggcga tgcacgacct cgagattcgc ggcacgggcg
    10621 aagtgctcgg cgacaagcaa tcgggcgaga ttcacgagat cggtttccag ctctataccg
    10681 acatgctgaa cgacgccgtg aaggcgctca aggaaggcaa ggagccggac ctcaccgcgc
    10741 cgctcgccgc gaccaccgag atcaatctgc acgcacccgc gattctgccg gcggactatt
    10801 gcggcgacgt gcaggaacgt ttgtcgctct acaagcgcct cgctaattgc gaacacaacg
    10861 attcgatcga cagcattcag gaagagttga tcgaccgctt cggcaagctg ccaccgcagg
    10921 cgcatgcgct cgtcgagacg catcgtctgc gcctcgcggc caagcccttg ggcatttcga
    10981 aaatcgatgc gggcgaaacc gtaatcggct tgcagttcat ccctaatccg cccattgacg
    11041 cgatgcggat catcgagatg gtgcagaaac acaagcacat caagctcgcg ggccaggaca
    11101 agctgcgtat cgaaacacgc agccccgacc tggccgtgcg ggtggcgacg gtgaaggaaa
    11161 ccttgcgcgc actcggaacg cccagccgcg gcacggcggc cgcggctgcc gtgcgctaac
    11221 gtatcgcgtc gtcgcgctgc tggcgcgccg gtattgcgtc aacgccggcg caactaccgg
    11281 cactcacgcc ggcacgataa aaacggcgta cgtctagccg tttttcattc atgctgcggt
    11341 gcgaaaacgc gtgccgcagc atacgggcga gctttttttg ggtatgatcg aaagactcaa
    11401 atgccggagt cttgtcatgt ctcatgtcgc tgaatcagcg cagtcctcgc caacgcccgc
    11461 ttctgaatcg aatgcttcct cgcccgccac gctccctgcg tcgcttccct gggtgacccg
    11521 tctcgtgtcg atggacacgg tgagccgcaa tccgaatctc ggtctgatcg agaccgtgcg
    11581 cgacgagctg cgcgcagtcg gcattgaagc cactctcact cacgaccaaa gcggcaaatg
    11641 ggccaatctg tttgcgacga ttcccgcgca cgacggcgag accaacggcg gcgtggtgct
    11701 gtcgggccat acggatgtcg tgcccgtaga cggtcagcaa tgggacagcg atccattcaa
    11761 gccggaaatc cgcggcgata aactgtatgg ccgtggcact tgcgatatga aaggcttcat
    11821 cggcgcggcg ctttcgctcg tgcccgacat gcaacgtacc aaactcgcca ggccgattca
    11881 cttcgcgttg tcgttcgacg aagaagtggg ctgcgccggc gcaccgctgc tgattaccga
    11941 tctgatgaag cgcggcgtga agcccgacgg ttgcattgtc ggcgagccaa ccagcatgcg
    12001 cccgatcgtg gcgcacaagg gcatcaacgc gtatcagtgc tgcgtgcgcg gccaggcggc
    12061 gcattcgtcg ctgacaccga agggtttgaa cgccatcgaa tacgccgcgc gcctgatctg
    12121 ctacatccgc gatatggccg atcaattccg cgcgcaaggc ccgttcgacg agctgtatga
    12181 cgtgcccttc accacggcgc aaaccagcac gatcgtgggc ggcaatgcga tcaataccgt
    12241 gccggccgaa tgcaaattcc agttcgaatt ccgcaatctg cccacgctcg atcctgaacc
    12301 gatcttcgcg cgcatcgatc aatacgcacg cgaaacgctg ctgccgaaaa tgttgcgcga
    12361 gcatccatct gccgccatcg agatcacgaa aatcgcggcc gcgcccggac tcgattcgtc
    12421 tgaacaagcc gcgatcacgc aactcgtgcg cgcgctgacc gccgaccagg acaagcgcaa
    12481 ggtcgcgtac ggaaccgaag ccggcctgtt ctcgctggcg ggcattccaa gcattgtgtg
    12541 cggccccggc gacatccagc aggcccacaa ggccaacgaa ttcgtcgcat tggatcagct
    12601 cgtggcatgc gagcgcttcc tgcaaaaatt catccacagc atgtcggttg aggcacacgt
    12661 acactgaaac cgaccgacgc aacaagagtc ttagacatac cgaggaaaaa ccacgcccac
    12721 gccatgtcaa ccgccacgcc gcagcacacc gaccatacga tcgacggcga gccgatcccc
    12781 acgctcgacg ccatcgccac acagcacttt gcgttgacgc cgtgggtggc gcgaacgccg
    12841 gtgttcgacc ggctcgactt tccgtcgctg gaaggcacgg tggtgaactt caagttcgaa
    12901 ttgctgcaag caggcggcag cttcaaggcg cgcggcgcgt tcaccaacct gctcgccctc
    12961 gacgaagcgc aacgcagcgc aggcgtcacc tgtgtatcgg gcggcaatca tgcggtagcg
    13021 gtggcgtatg cggccatgcg tcttggcatc agcgcgaagg tcgtgctgtt tcgcgcggcc
    13081 aatccggcgc gcgtcgcctt gtgccggcaa tatcacgccg acatcgtgtt cgcggaagac
    13141 attgccgaag cgttcgaact cgtgcgcggt atcgaggcag aagaaggccg ctatttcgtg
    13201 cacccgttca acggttatcg cacggtgctg ggttcggcca cgctcggcta tgaatgggcc
    13261 acgcaaacac cggatctcga agcggtgatc gtgccgatcg gcggcggcgg tctcgcagcc
    13321 ggcgtggcca ctgccatgcg gctcgccaat ccgcgcgtgc acgtgtacgg cgtcgaaccg
    13381 gaaggcgcgg atgcaatggg caaaagcttc gcggccaatc acacggtcaa gatgggccac
    13441 atgcacggca ttgccgattc gctgatgtcg ccgcataccg aggaatacag ctacgagttg
    13501 tgccgccgtc atatcgacca gctcgtgacc gtatccgacg accagttgcg cgcggccatg
    13561 ctgaccttgt tcggacaact taagctggcc gtcgaaccgg cctgcgccgc cgcaacggct
    13621 gcgctgctcg gaccgctgcg cgaatccctg caaggcaagc gcgtaggcgt gctgctgtgc
    13681 ggcaccaata ccgacccggt cagttttgcc gcgcatatcg aacgggcccg ccatagccag
    13741 tcacagtttc cagaataaat ggccttgagt tctttccgtg cgaaagcctg gccgaaagac
    13801 tagcctatct ttctcagatt ggtatgattc aattctcgac ttctgggaga aacgcatatg
    13861 agcatgatca aggagttcaa ggaatttgcc ctcaaaggca acgtaatgga tctcgccgtc
    13921 ggggtgatta tcggcggcgc attttccacc atcgtcaatt caattgttaa agacctgatc
    13981 atgccggttg tcggcgtcgc caccggcggc ctcgattttt ccaacaagtt cgtgcgcctc
    14041 ggcgatattc ccccgagctt caaaggcagt cccgagtctt ataaagactt gcaaacggcc
    14101 ggtgtcgcgg tgttcggtta tggctcgttc atcactgtgg ccattaattt cctgatcctc
    14161 gcgttcatca ttttcctgat ggtcaagttc atcaacaatc tgcgcaagcc gcttgaagcc
    14221 gcaccggcag agccgccgcc tccgccggaa gacgtcctgc tgctgcgtga aatccgcgat
    14281 tcgctgaaaa acgcgccgcc gcgctaggca accgaagcca cgctgagtca cattaatctg
    14341 ggttgacaat tcgcgccggc acattacccg gcgcgaataa aaaaggcctg ttgcgaagaa
    14401 aatcgccaca ggcctttttc atgctccgcg cacattacgt cgatccaatc gacgcagcga
    14461 accttttaag cgtcgttaag cgttcttttc caccctcaga ctcacggcgc tttcgatcat
    14521 cgtttcgagc tgtccgaaat tgacaggctt ggtcagatgc gaagtaaagc cggcgctcag
    14581 acaacggcgc acatcttcat ccgtaccgaa gccggttaat gcgaccgccg gcgcatccga
    14641 atgctcgcga aacgctttga cgaaatcgag gccggtgcca tcgggcaaac cgacgtcgct
    14701 aatcaccaga tcgaacgtct gcgactgagt ggcggccagt gcgtcgtcca cacggcccac
    14761 tgtcgtcacg tcgtgaccca ggctgcgaat caactgctcc atcacttcgg cggtatccac
    14821 gtggtcttcg atcaacagaa tggtcagcag gcctggagga tgcacctcat cgggcgttgc
    14881 aacgggtgtt gattcgaccg gcgcggcagc ggtgggcagc gtaatcgtga acgtcgcgcc
    14941 gcagtgcgcg cccgggctct gggcgatcac cgtgccgccg tgcacatcgg tcagtgcctt
    15001 ggtgatcgca agcccaaggc ccaggccacc gaactgccgc gtcatgttct ggttgccctg
    15061 ttcgaacgca ttgaacagtt tgccgatctg ctcgggctca atgccgatgc ccgtgtcttc
    15121 aaccgagatc tgcacgtgca tgcgttcatc gcgcgtgcgc acatagatat ggccgccgtc
    15181 gggcgtgaac ttggcggcgt tgcgaatcag gttccacagc atctgttgca agcgcgcgcg
    15241 gtcggcgagc acgtagtggt tgtgagcgtt cttgcagacg tgcacgtcct gctgcttgac
    15301 ctgaatctcg ctgcgaaaga gttcgagcac actgtccatc acttcgtgca cgtcgacggt
    15361 ttcgagcgac agacgcagct tgccgttcgc cacgcgcgtg agatcgagca gatcgtcgat
    15421 caggcgcgcc tcgagttcga cgttacggcg gatcatacgc acgctcgcgc gcgcctgatc
    15481 cggtagaccc ggaatcatct ccagcacgct ggcgcccgcc aataccggcg taagcggcgt
    15541 gcgcaattca tgcgacagca tggcgagaaa gcggtccttc gcacgattgg cttcttcggc
    15601 gatctgccgg gcggcctgct ccgaggcgag cagccgttcg cgttcctcga ttgcctcgcg
    15661 ctggggatga atgtccgtgc agctgccgaa ccatttgctg acgttgcctt cggcgtcgcg
    15721 cgtggagatc gcgcgggcgt cgaaccagcg atattcgccg tcatggcggc gaatccgcag
    15781 ttcgtgccga taatcgccgg cacggctcgt cactgccttg agccaggtac gacgaatctc
    15841 ttcgcgatca tcgggatgca ccgcttcgag ccacgcgaga ccgtacgaac ggttttccgt
    15901 caagcccgtg tagtcgaacc actgcttcga aaggaaatcg caatcgcccg cggcattgca
    15961 ggtcagcacg aggtgtggca acgcctctga caaggtacgg tagtgctgtt cgcggtcgcg
    16021 cagcagcaac gcttcgtccg acgccttctg caagcgcacc tgcgagagca cccgctccac
    16081 cgcctcgggc aggtaatcga ggtagtcgcc ggatttcggc accacatcgg atacgccggc
    16141 gcgcaatgcc tcgatcacgc gagattcgtc agtgaaaccg gtcacgagga tggcgggaat
    16201 ccgcacacct tccgcgcgca agcggcgaaa gaagtcgagg ccgctctccg caccgctcaa
    16261 ctggtagtcg agcacgagca ggtcgggcgc gcccgtggcg agccgctcgc gggcggcctc
    16321 gacacccggg cagatggcga cacggcagcc ggcacgttcg agcgacttgc gcgcgagccg
    16381 caagatgcct tcgtcgtcgt cgacgaccag cacatacgcg gcggactgcg tgggcacttc
    16441 ctgggtcatg cggagtcttc tttggctagg tctggtgtgg ttatcttgtc acgggctgct
    16501 ttcggagcgc cttcagggtt cttctatcac ggtgccgtca agcggtgacc cggcggcagc
    16561 ttgaccacct gcaggaagaa gccgagacgc cgcaccgctt cgatgaacgc ctcgtactcg
    16621 accggcttgg tgatatagac gttgcagccg agctcgtagc agcgcgcgat ttcacgtgga
    16681 tcgtcggtcg tggtcagcac gatcacgggc accgcggcgg tctgtggcga atccttcagc
    16741 cgccgcaata cctcgaagcc gtccacacgc ggcattttca gatcgagcag cacgacgaaa
    16801 ttcgtcagat cctcgcgcgg cgggtatggg ctcgcagcgg ggtccgccgg cacggggccg
    16861 aagaaataat cgagtgcctg ctggccatcg cgaaagcgca cgaaaccgtt cgaaatacca
    16921 gcacggcgca gattgcgctc gaccagcgtg gcgtggccgt cgtcatcttc gatcagcacg
    16981 atgctgaccg tttccccgtg actcatatgc cctccgtgct gacgatccat tcttgcataa
    17041 tgcttttagc cggccacagt gtcccctgcg gcggattacg gctcaatccc ggcttaacct
    17101 ggcttaacct ggcttaaccc ggctcaattc tggctcaatg gcgcgcgggc tgttccggca
    17161 gcacgacgaa aaaagtcgac cccgcgcctt ccgccgactc gacccacacg cggccgccat
    17221 gccgttccac catccgccgc accaccgcga ggccgatgcc gtcgccatcc gcgacgtcgc
    17281 cgtgcagacg ctgaaacgcc cggaacacct tcgacatata cgccgccgga atgccgaggc
    17341 cgttgtcgcg cacatagtag gtgcgcgtgc gcagcgcgcg cggctccctc tcgtcgaccg
    17401 cttccagctc cggctccagc gcgccgatct cgatgcgccc atggcgcgcc gggtcgagat
    17461 aattgagcgc gttgccgatc agattgctga aaatctgctc gatcgcgccc ggatcgcccc
    17521 atgcgggcgg caactcgcgc acggtgatca ccgctctgcg ctgcccgatc gcgctctgca
    17581 gcgcatcgac cacgcggccc actacccgcc cgacactcac ccgctgccac tggtattcga
    17641 gccggccggc acgcgaaatc cgcaataacg cgtcgatgat cgacgccgct tgggtcaccg
    17701 cgctgcgcaa atattgcagc gattcgcgca cgtcgccgtc gaggatatgc accatgcgcc
    17761 ggtgctcggc ctccggcagt ttcgccgccg cgacgatgct gtccagttcg tcgcacgaca
    17821 cctgcaattc tctcgaaaag ccctgcagat tcaccaacgg cgagcgcaga tcgtgcgaca
    17881 cgctgtagat gaacatttca ttatcttgcg tctcctgacg cagttcctcg ttgacgcccg
    17941 ccaactcccg cgcgcgcgcc tcgagccgcg ttttcagcat ggcctg
//
LOCUS       DATASH010000356        14077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4997482,
            whole genome shotgun sequence.
ACCESSION   DATASH010000356 DATASH010000000
VERSION     DATASH010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 14077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14077
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4997482"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..154
                     /locus_tag="VHV99_10045"
     CDS             <1..154
                     /locus_tag="VHV99_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006399767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX3635814.1"
                     /translation="RRLAERIAGKGAKQTLPIFTSPLPGHPFAPFRRLGQRMLYQLYF
                     RRDEKP"
     gene            387..1817
                     /locus_tag="VHV99_10050"
     CDS             387..1817
                     /locus_tag="VHV99_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX3635815.1"
                     /translation="MQVSANKSELKTGLKERHMTMIALGGVIGAGLFVGSGVVVQQAG
                     PAAVLSFLITGGLIVLVMRMLGEMACAMPAVGSFYEYARLAFAGKRGPGKLAGFLTGW
                     MYWYFWVIVVAVEAVAGAKLVQFWLPDTPAWAISLVLLVVLTATNLISVGSYGEFEFW
                     FASIKVAAIVVFLFLGGLYVLGLWPASMHTTSVMPTLLSHGGLMPKGIGPVLSGAVAA
                     TGFYFGAEIVTIAAAEAHEPVKAVAKATNSVITRVLIFYVGSILLVVALVPWDSPKMA
                     TPYVSALDAMGIPAAANVMNAIVLTAVLSALNSGLYAASRMMFALTRHGDAPAALARV
                     NRRGVPVRAILVGTLFGYVSVVMSYVSPDKVFAFLVNSYGTVAIFVYVLIAVSQLKLR
                     ARLEREAPEMLRVRMWCYPYLTCFAILGMIGILVAMAFIPDQRTPLWLGVVSLGVLLV
                     AYAWRARKRGAASSEPEFVEYRPHTH"
     gene            complement(1993..2835)
                     /locus_tag="VHV99_10055"
     CDS             complement(1993..2835)
                     /locus_tag="VHV99_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006399762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX3635816.1"
                     /translation="MRDTTRSPSPAHEPTDDSRVLRALNVLEQLASAGQPSSLSQLSA
                     RLQIPKATLMRLIESLEARGYVTHMPDSRGADRAIALGPRAAQLALTTLANSTFTRAC
                     RSLLRTLVDALGETCNLTVLDGDTVLYVERVETSEPLRLHMQPGTRVPLHCTASGKLF
                     LSQMTPAERGLVIRRLPLKAMTYRTLTDIDLLEAELDRLAVRGIGVDNEEFVRGMVAV
                     AVPVRNVEDGRVLAALAVHSPTARASLEDLLKAVPKLKEIAVRIGPLLQPATMATAAV
                     AASK"
     gene            3059..4720
                     /locus_tag="VHV99_10060"
     CDS             3059..4720
                     /locus_tag="VHV99_10060"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012217226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HEX3635817.1"
                     /translation="MVRSFDYVVVGAGSAGCVLANRLSENGRYSVCLLEAGPPDRFLW
                     IHIPIGYGKTMFHPVYNWGFYTDPDPNMHDRKLYWPRGRTLGGCSSINGLIYVRGQKE
                     DYDHWAALGNRGWSWKECLPYFRRLEHNELGESPTRGVGGPLWASTIKQRHELVDAFI
                     AASNRLGVPTVDDFNQGDQEGVGYYQLTTRHGWRCSTAVAYLKPARSRPNLQIETDAL
                     ASKILFEGTRATGIRYLQHGKEHEVRAHREVVLTAGALQSPQLLQVSGVGPAKLLNEF
                     GIPVVANRAGVGENLQDHLQVRLTYEVTRPITTNDLLHSWTGRAKMGLQWALFRDGPL
                     AVGINQGGMFCRALPDEAKTPDIQFHFSTLSADTAGGDVHAFPGCTYSICQLRPESRG
                     SLRIRTNDMRDAPSIQPNYLATDLDRRTTVAGVRFARRVAATEPMAALMKREVRPGRE
                     AQSDDELLDFCREYGQTIFHPSGTAKMGVASDPAAVVDERLRVYGTQGLRVVDCSIMP
                     TLVSGNTNVPIVMVAEKASSMILEDARAGDRPAGQVQSRSMEAHY"
     gene            4831..6135
                     /locus_tag="VHV99_10065"
     CDS             4831..6135
                     /locus_tag="VHV99_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3635818.1"
                     /translation="MAIDQRVIRRVAFASVIGATVEWYDFFLYGVVAGIVFNKLYFPA
                     HDPLVSTMLAYGTFAVGFVTRPLGGVIFGHFGDKVGRKSALICTLVIMGISTAGVAFV
                     PTFAQIGIWAPILLLTLRVLQGIGLGGEWGGAVLMAYEYAPPNRRGLYASLPQIGLSI
                     GLCLASGIVAGISRLLPEEAFLSWGWRVAFAASLLLVLLGLYIRVKVMETPEFLALKR
                     NRRAVKIPFVDMITRYRSSVLLGMGARWIDGVFFNIFAVFMIGYLTQRLSMSRTEALT
                     GVMIAAMVMCFFIPFFGRMSDLMGRARVYRWGSLICGVSVLPAFWLMETQAGNTLAVW
                     LGVVIPFGIFYAMVYGPEAALFAELFDPEVRYTGISFVYQFSGIFASGLTPIIATALL
                     RLDGGKPWYVCVYVILSALLSAWAARAMERRAKPFDEMATAR"
     gene            6284..7279
                     /locus_tag="VHV99_10070"
     CDS             6284..7279
                     /locus_tag="VHV99_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3635819.1"
                     /translation="MDIATGKPDRMVSVRTVFGIDSSLMVPAFSERDDHVPEIDEVYR
                     FNPEVTLAILAGFSRDRRVMVQGLHGTGKSTHVEQVAARLNWPCVRVNLDGHISRLDL
                     VGKDAIVVRDDLQVTEFQEGIVPWALQRPVALIFDEYDAGRPDVMFVIQRILERDGKF
                     TLLDQNRVIHPHPYFRMFATTNTVGLGNLNGLYHGTQVLNHAQMDRWNVVATLNYLPR
                     AEEAGIVLARVPELADEAGRALIESMVSVAELTRKGFASGDLSTLMSPRTVINWAENC
                     QIFGDPAMAFRLTFLNKCDEAERSIVAEYFQRCFGTELEPAGVGGDAQHTAESSR"
     gene            7534..9213
                     /locus_tag="VHV99_10075"
     CDS             7534..9213
                     /locus_tag="VHV99_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012405412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt chelatase"
                     /protein_id="HEX3635820.1"
                     /translation="MRALTGDASLHYREGRLCRDLRPLPLHAPHLRTDPQQDDFASLR
                     GAADGAAMRLMHSDAALHSQLCPTDPVERLVFELLEQLRCETQTPPGMPGLVRNLRER
                     FEAWSRAFYHAGLAEDHLGILLFTVAQIAWSRLTGWPVLEQIEGPIEATRAAIVPVLG
                     GSLAGLRRHRQEQDAFAVHALEMARLVAGMIRAARADALGETQDDVEESDDKAPRTAF
                     SLWFDVEDDEYKDMAVALSGRSRVLEASEQGYRAYTTRHDRELVAGTLVRKALLDQFR
                     EQLDERVAVQGINVARLAHALKTALAIPQRDGWSFGEEHGRIDGRRLAQLVSSPAERR
                     LFRLERNTLRADCVVGFLIDCSGSMKAHIEPVAIMVDILTRALDQAGVTTEVLGFTTG
                     AWNGGRARLDWLAQGRPPHPGRLNELSHLVFKDAGRSWRRARADIAALFKADLFREGV
                     DGEAVDWACGRLVARSEARRILIVISDGSPMDSATGQANDAFYLDNHLKAVVARHDAL
                     RDVEVLGLGVGLDLSPYYRRCLAVDLSVPPDMKLFAEIVGWIGARGRGGKD"
     gene            complement(9395..10435)
                     /gene="pta"
                     /locus_tag="VHV99_10080"
     CDS             complement(9395..10435)
                     /gene="pta"
                     /locus_tag="VHV99_10080"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEX3635821.1"
                     /translation="MKAINRIIERARTSPRRIVLCEAEDPRMLQAAHRATREGIARIV
                     LVGPAARIRAAAAGQNIELAGMELVDPATSLFTPSFSEALFALRSKKGMTLEEAKQEV
                     LKPLCFANLMVRLGHADGSVAGCVHTTADVVRTAIQIIGIHPSFKLVSSFFLMMLCEP
                     FHTLKGGLIFSDCGLVVDPNAGELAQIAMAAADSAQSLLMEAPRVAMLSFSTSGSARH
                     AAVDKVVEATRQVRAARPHLAIDGDVQLDAALVSEIALRKIDHSQVQGHANVLIFPSL
                     EAGNIGYKLAERIGDAKAIGPLLQGLNKPANDLSRGCSADDVYYVIAVTAVQAQAADE
                     PVSAHRMPHHAS"
     gene            complement(10620..12422)
                     /gene="xsc"
                     /locus_tag="VHV99_10085"
     CDS             complement(10620..12422)
                     /gene="xsc"
                     /locus_tag="VHV99_10085"
                     /EC_number="2.3.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006403857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050487 - sulfoacetaldehyde
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0019529 - taurine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoacetaldehyde acetyltransferase"
                     /protein_id="HEX3635822.1"
                     /translation="MSDHTPVSGPQAMTPSEAFVETLAANGVTDMFGIMGSAFMDAMD
                     IFAPAGIRLIPVVHEQGAGHMADGYARVSGRHGVVIGQNGPGISNCVTAIAAAYWAHS
                     PVVMITPEAGTMGIGLGGFQEAKQLPMFQEFTKYQGHVTHPARMAEFTGRCFDRALAE
                     MGPTQLNIPRDYFYGQIKAEIPQPQRLDRGPGGDQRLNEAAELLAQAKFPVIISGGGV
                     VMADAIEECKALAERLGAPVVNSYLHNDSFPADHPLWCGPLGYQGSKAAMKLIQRADV
                     VVALGSRLGPFGTLPQHGMDYWPQNAKIIQIDADHKMLGLVKKISVGICGDAKAAAVA
                     LTQRLEGRTLACDASREDRAGHIAAEKAAWEKELDGWTHERDPYSMDMIEEQKNERTP
                     GGGHYLHPRQVLRELEKAMPDDVMVSTDIGNINSVANSYLRFTKPRSFFAAMSWGNCG
                     YAFPTIIGAKVAAPHRPAVSYAGDGAWGMSLMETMTCVRHNIPVTAVVFHNRQWGAEK
                     KNQVDFYNRRFVAGELDNQSFAGIARAMGAEGVTVDRLEDVGPALKRAIDAQMNHGKT
                     TIIEIMCTRELGDPFRRDALAKPVRLLDKYKDYV"
     gene            complement(12628..14076)
                     /gene="phnY"
                     /locus_tag="VHV99_10090"
     CDS             complement(12628..14076)
                     /gene="phnY"
                     /locus_tag="VHV99_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006162122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde dehydrogenase"
                     /protein_id="HEX3635823.1"
                     /translation="MAKVWPAPQVRHEALRIGGELIRRAEIIEVFNPYTNELVGTVPK
                     ATLADVRRAFAWAHQYRARLTRYERSQILLRAADGVRRRTEEIAGLITAESGLCKKDS
                     LYEAGRVADVMTFGANEALKDDGQVFSCDLTPHGKKRRVYTQRDPLLGVISAITPFNH
                     PMNQVAHKVVPSIATNNRIVVKPSEKVPLSTYLLADILYAAGLPQEMLQVITGDPKDI
                     ADELITNEHVDLITFTGGVQIGKSIASKMGYRRAVLELGGNDPIIVMEDADLEEASTL
                     AVSGSYKNSGQRCTAIKRILVHEAVADRFVELLVEKSRAIRYGDPADPATDMGTVIDE
                     AAAKFFEAQVNDAVSRGATLLLGNRREGALYSPTVLDHVSPDMPLVKYETFGPVSPVI
                     RFRDIDDAIRISNSTDYGLSSSVCTNRLDYITRFIAELEVGSVNVREVPGYRLELTPF
                     GGIKDSGLGYKEGVQEAMKSFTNVKTYSLPWD"
BASE COUNT         2443 a         4260 c         4640 g         2734 t
ORIGIN      
        1 ccgccggctc gccgagcgga tcgccggtaa gggggcgaag cagacgctgc cgatctttac
       61 gtcaccgttg cccggccatc cgttcgcacc gttccgtcgc ctcggacaac ggatgctcta
      121 tcagctctat ttcaggcgcg acgaaaagcc gtgaaggcgg cctacgtacc gcgtcgtccg
      181 cggtacgtag cgcaccattt accgtgcctc gcgcgcacgg caccgcgcag gcagccaggt
      241 ctgcgcattc agtcataaga aaaaaacaat cagcagcgag gcgcgcaagc gtcctcgctc
      301 gatccggatt gacggcgccc cggtctggcg gctgcatccg cagcacggca cgacaggcgt
      361 ctgaataagc aggatatgga ggtgacatgc aagtatcagc gaataaaagc gaattgaaga
      421 ccggcctcaa ggagcgccac atgaccatga tcgcgctagg cggcgtgatc ggcgcgggcc
      481 tgtttgtcgg cagcggcgtg gtggtccagc aggccggccc ggccgcggtt ctgtcgtttc
      541 tgattaccgg cggcctgatc gtgctggtca tgcgcatgct cggcgaaatg gcctgcgcga
      601 tgccggcggt gggttcgttc tacgaatacg cgcgtctcgc cttcgccggc aagcgcgggc
      661 ccggcaaact ggccggcttt ctgaccgggt ggatgtattg gtatttctgg gtcatcgtcg
      721 tcgcggtcga agcggtggcc ggcgcgaaac tcgttcagtt ctggttaccc gatacgccgg
      781 cgtgggcgat cagtctcgtg ctgctggtgg tgttgacggc gaccaacctg atctcggtgg
      841 gcagctacgg cgaattcgag ttctggttcg cgtccatcaa ggtcgcggcg atcgtggtgt
      901 ttctgttcct cggcggtctg tatgtgctgg ggctgtggcc tgcgtcgatg cacaccacct
      961 ccgtgatgcc cacgcttctt tcgcacggcg ggctgatgcc gaaggggatt gggccggtat
     1021 tgagcggcgc ggtcgcggcg acaggttttt acttcggcgc ggagatcgtc acgattgcgg
     1081 cggccgaagc gcatgagccg gtcaaggcgg tcgccaaagc caccaactcc gtgatcacgc
     1141 gcgtgctgat tttctatgtc ggctcgattc tgctggtggt ggcgctggtg ccctgggatt
     1201 cgccgaaaat ggcgacgccg tatgtgagcg cgctcgacgc gatgggtatt ccggccgccg
     1261 cgaacgtcat gaatgcgatc gtgttgacgg cggtactgtc cgcactcaat tccggcctct
     1321 acgcagcctc acggatgatg ttcgcgctga cccgtcatgg cgacgcgccg gcggcgctcg
     1381 ccagggtcaa tcgtcgcggt gtgccggttc gcgcgattct ggtcggcacc ttgttcggct
     1441 atgtgtcggt ggtgatgtcc tatgtatcgc ccgataaggt gttcgccttc ctcgtcaatt
     1501 catacgggac ggtggccatt tttgtctacg tgctgatcgc ggtgtcgcaa ctgaaactgc
     1561 gcgcgcggct cgagcgcgag gcgccggaga tgctgcgcgt gaggatgtgg tgctacccgt
     1621 atctgacctg tttcgcgatt ctcggcatga tcggcattct ggtggccatg gcgttcatac
     1681 ccgatcagcg gacgccactg tggctgggcg tcgtgagtct gggtgtgctg ctcgtggcct
     1741 atgcatggcg cgcccgcaag agaggggcag cttcttctga gccggaattt gtggagtatc
     1801 ggccgcacac gcattgaggg ctgcttctgc ggttcgcatc tcagcaattc agcagtgcag
     1861 cagtgcagca gtgcagcagg cgtcgctcgc gaccaaccgc tgcactgcgg cgacacgtta
     1921 ccggctcgtg ccgatgcggt cccgggaggc acacgcactc cgtctccgtc tccgcgaagg
     1981 agcgaaagag cactacttcg acgcagccac cgcggccgta gccattgtcg caggctgcaa
     2041 cagcggcccg atgcgcacgg cgatttcttt cagcttcggt accgctttca gcaagtcctc
     2101 cagactggca cgcgccgtgg gcgaatgaac ggccaacgcg gcaagcacgc ggccgtcctc
     2161 gacgttgcgc accggcacgg caaccgccac catgccgcgc acgaactcct cgttgtcgac
     2221 accgatgccc cgcacggcga ggcggtcgag ttccgcttcc agcagatcga tatcggtcag
     2281 cgtgcgatac gtcatcgctt tgagcggcaa gcgccggatg acgagcccgc gctcggcggg
     2341 tgtcatctgc gaaagaaaca gcttgccgct cgccgtgcag tgcagcggca cgcgcgtgcc
     2401 cggttgcata tgcagacgca agggttcgct cgtctcgacg cgctcgacgt acaacaccgt
     2461 atcgccgtcg agcacggtga gattgcaggt ctcgccgagc gcatccacga gcgtgcgcag
     2521 caacgagcgg caagcgcgcg taaacgtgct gttcgcgagc gtggtgagcg cgagctgcgc
     2581 cgcgcgtgga ccgagtgcga tcgcgcgatc ggcgccgcgc gagtccggca tatgcgtgac
     2641 gtaaccccgt gcttcgagcg attcgatcag acgcatcaag gtcgccttcg gaatctgcag
     2701 acgtgcggac agttgagaga gcgaagacgg ttgtcccgct gaagcgagct gttccagcac
     2761 attgagggcg cgcaggacgc ggctgtcgtc ggtgggctcg tgagcgggtg agggcgaacg
     2821 cgtggtgtct ctcattggcc ttcccgaggc ggatgaagat gaatgcagcc cgaacggatc
     2881 agtgacaacc cggatgatcg cgcgtgggcg gatcgaatag tgaaacgaaa aagtcggttt
     2941 tcgtaccgga ttactgaatc ctaaccggat ttgcttgaag tcgaaacagc catccaataa
     3001 attgggacga aatggaccgt tttttaaata caagacaagc atcagcacgg agacatcaat
     3061 ggtacggagt ttcgactacg tggtcgtcgg cgcgggatcg gccggctgtg tactggccaa
     3121 ccgcctctct gaaaatggcc gctactcggt gtgtctgctc gaagccggtc cgccggatcg
     3181 ttttctgtgg atccatatcc ccatcggtta tggcaagacc atgttccatc cggtctacaa
     3241 ctggggcttc tacaccgacc ccgatccgaa catgcacgat cgcaagctct actggccgcg
     3301 cgggcgtacc ctgggaggct gcagttccat caacggactg atttacgttc gcggccagaa
     3361 ggaggactac gaccactggg ccgcgctcgg caatcgtggc tggagctgga aggagtgcct
     3421 gccttacttc cggcggctcg aacacaatga gcttggcgaa tcgcccacgc gtggcgtggg
     3481 cggcccgttg tgggcctcca cgatcaagca gcgccacgaa ctcgtggatg cgttcatcgc
     3541 cgcgtcgaat cgcctggggg tcccaaccgt cgacgacttc aatcaaggcg atcaggaagg
     3601 cgtcggctat taccagttga ccacgcggca tggctggcgg tgttcgactg ccgtcgctta
     3661 tctcaagccc gcgcgcagcc gtccgaatct gcagatcgag accgacgcgc tggcgtcgaa
     3721 gatccttttc gagggcacgc gcgccacggg tattcgctat ctacagcacg gcaaggaaca
     3781 cgaggtgcgc gcacaccgcg aggtagtgct gacggcgggc gcgctgcagt cgccacaatt
     3841 gctgcaagtg tcgggcgtcg ggccggcgaa attgctcaat gagtttggaa ttcccgtggt
     3901 cgcgaaccgg gccggcgtcg gcgagaatct gcaggaccat ttgcaagtac ggctgactta
     3961 cgaagtgacg cggccgatca cgacgaacga cttgctgcac tcctggacgg gccgcgcgaa
     4021 gatgggactg caatgggcgc tgtttcgcga cgggccgctt gcggtcggca tcaatcaggg
     4081 cggcatgttc tgccgcgcat tgccggatga ggcgaaaacg cccgacatcc agttccattt
     4141 ctccacgctc tcagccgata ccgcgggtgg cgacgtccac gcgtttccag gctgcaccta
     4201 ttcgatttgc cagctgcggc ctgagtcgcg cggcagcttg cggattcgca cgaatgacat
     4261 gcgcgacgcc ccgtcgattc agccgaatta tctggcgacc gatctcgacc gccgcaccac
     4321 ggtcgcgggt gtgcgtttcg cgcgccgggt ggccgctacc gagcccatgg cagcgctgat
     4381 gaaacgtgaa gtgcgtcccg gccgggaggc gcaaagcgat gatgaattgc tggacttttg
     4441 ccgcgaatac ggccagacga tttttcatcc gtccggcacc gcgaagatgg gcgtcgcgag
     4501 cgatccggcc gcggtggtcg acgaacggct gcgcgtgtat ggcacgcaag gcttgcgggt
     4561 ggtcgattgc tcgatcatgc cgacgctggt ctccggcaat accaatgtgc cgatcgtgat
     4621 ggtcgctgaa aaagcgtcca gcatgatcct cgaggatgcc cgtgccggtg accggccggc
     4681 ggggcaagtg cagtcgcgga gtatggaagc gcattactga cgcagaaagc aggtaacgat
     4741 attcgcaccc ccgaggagga gacagacagg gggctggaaa atgtgctgcg atgccgggac
     4801 ggcacgcggc catccattgg aggagacgct atggcaatcg atcagcgggt tatccgccgc
     4861 gttgcattcg cgagcgtgat tggggcgacg gtcgaatggt atgacttttt tctgtacggt
     4921 gtcgtcgccg gtatcgtgtt caacaaactg tattttcccg ctcacgatcc actcgtctcg
     4981 acgatgctcg cgtatggcac gttcgccgtc ggcttcgtga cgcggccgct cggcggtgtg
     5041 atcttcggcc acttcggcga caaggtgggg cgcaagtccg ccttgatctg cacgctcgtc
     5101 atcatgggga tttcgacggc cggcgtcgcc tttgtgccga ccttcgctca gatcggcatc
     5161 tgggcgccga ttctgttgct caccctgcgt gtgttgcagg gcatcgggct cggcggcgaa
     5221 tggggcggcg cggtgttgat ggcctatgaa tatgcgccgc cgaaccggcg cggtctgtac
     5281 gcgagcttgc cgcagatcgg cttgtcgatc ggcttgtgtc tcgcctcggg cattgtcgcg
     5341 ggcatctccc gcttgctgcc ggaagaggcg tttctctcgt ggggctggcg agtcgcgttt
     5401 gccgcttcgt tgttgcttgt gctgctcggc ctctacatcc gcgtgaaggt gatggagacg
     5461 cccgagtttc tggcgctcaa gcgcaaccgc cgcgccgtga aaatcccgtt cgtcgacatg
     5521 atcacacgct atcgcagttc ggtcctgctc ggcatgggcg cgcgctggat cgacggggtg
     5581 tttttcaaca tcttcgcagt gttcatgatc ggctacctta cgcaacggct ttcgatgagc
     5641 cgcacggaag cgctcaccgg cgtgatgatc gcggcaatgg tgatgtgctt cttcattccg
     5701 ttcttcggcc ggatgtcgga cctgatgggg cgcgcgcgcg tgtatcgctg gggttcgctg
     5761 atctgcggcg tctcggtgct gccggcgttc tggctgatgg agacgcaggc gggcaatacg
     5821 ctggccgtgt ggctcggcgt agtgatcccg ttcggcatct tctacgccat ggtctacggt
     5881 ccggaagcgg cgctctttgc cgagctgttc gatccggagg tgcgttatac gggcatctcg
     5941 ttcgtgtatc agttctcggg catcttcgcg agcggcctca cgccgatcat cgcgacggcg
     6001 ctgctgcgcc tcgacggcgg caagccctgg tacgtgtgcg tctatgtgat cctgtcggcg
     6061 ctgctttcgg catgggcggc gcgcgcgatg gaacggcgcg cgaaaccctt cgacgagatg
     6121 gcgaccgcgc gctgagggcg cgcccgccgg tgcacctgga cgaaatgccg ctcaccgcga
     6181 gcgattctgt ttaaagtaaa aacggaacaa gtcgcaccga aaactgaaaa tagcgtcggc
     6241 ggcgccgaac gaatgtcccc gcagcgggag ggagaatgaa gtgatagata tcgcgactgg
     6301 caaacccgac cgaatggtct cggtacggac cgttttcggc atcgattcca gcctgatggt
     6361 gcccgctttc agcgagcgcg acgaccacgt gccggagatc gacgaggtct accggttcaa
     6421 tccggaggtg accctggcga tcctggccgg ctttagccgt gaccgtcggg tgatggtgca
     6481 gggcttgcac ggcaccggga agtcgactca cgtcgaacag gtcgccgcgc ggctcaactg
     6541 gccatgcgtg cgcgtcaatc tcgatggcca tatcagccgc ctcgatctgg tgggtaaaga
     6601 cgcgatcgtg gtgcgcgacg acctgcaggt gaccgagttt caggagggca tcgtgccgtg
     6661 ggcattgcag cggccggtcg cgctgatctt cgacgaatac gacgcgggcc gtccggacgt
     6721 gatgttcgtg atccagcgca ttctcgagcg tgacggcaaa ttcaccttgc tcgaccagaa
     6781 tcgcgtgatt catccccatc cgtatttccg catgttcgct accacgaaca cagtggggct
     6841 cggcaatctg aacggcctgt atcacggcac ccaggtgctg aatcacgcgc agatggaccg
     6901 ctggaacgtg gtggccacgc tcaactatct gccgcgcgcg gaggaggccg gcatcgtact
     6961 cgcgcgtgtg ccggaacttg ccgacgaggc gggacgcgcg ctgatcgaat cgatggtgag
     7021 cgtggcggaa ctcacccgca agggcttcgc cagcggcgat ctgtcgacgc tgatgtcgcc
     7081 gcgcacggtg atcaactggg cggagaactg ccagattttc ggcgaccccg cgatggcgtt
     7141 tcgcctgacc ttcctgaaca agtgcgacga agccgagcgg tcgatcgtgg ccgagtattt
     7201 ccaacgctgc tttggcacgg aactcgagcc cgcgggcgtg ggcggtgacg cgcaacatac
     7261 agcggaatcg agtcgatgac gtctgaacgg caggtggatt tgcgtcgagt ggacccgcgg
     7321 cgggcggact tgtgccaggc ggatccgcgc caggcagacc tgcgtcaagt ggacccgcgc
     7381 caggcggact tgtgccaagt ggacccgcgt caggcggact tgcgtcagcc aaccccgcgt
     7441 caggcggcgc caagggagtc caccctgaaa gagccaaccc ggcgccaggc gacgcttcgc
     7501 gcagaacagc gcgaggctct atgcgcggcg gccgtgcgcg ccctcacggg tgatgcctcc
     7561 ttgcactacc gcgagggccg tttgtgccgc gacctgcggc ctttgccgtt gcacgcgccg
     7621 catctgcgca ccgacccgca acaggacgac tttgcatccc tgcgcggcgc cgccgatggc
     7681 gccgccatgc gactcatgca ctccgatgcg gctttgcaca gtcaactttg cccgaccgat
     7741 cccgtcgaac gactcgtgtt cgaattgctc gaacagctgc gttgcgaaac gcaaacaccg
     7801 cctggcatgc cgggcctggt gcgaaatctg cgcgagcgtt tcgaagcctg gtcgcgagcc
     7861 ttctaccacg ccgggcttgc cgaggaccat ctcggcattc tgttattcac ggtggcgcag
     7921 atcgcgtggt cgcgcctgac cggctggccc gtgctggaac agatcgaagg tccgatcgaa
     7981 gccacgcgcg cggccatcgt gccggtgctg ggtgggtcgc tggcgggtct gcgccggcat
     8041 cggcaggagc aggacgcgtt cgctgtccat gcactggaaa tggcgcggct ggtcgccggg
     8101 atgatccgtg ccgcccgcgc cgacgcgctc ggcgagacgc aggacgacgt ggaggagagc
     8161 gacgacaaag ccccgcgcac ggcattctcg ctctggttcg atgtcgagga cgacgagtac
     8221 aaggacatgg ccgttgcgct cagcggcagg agccgcgtgc tcgaagcatc ggagcagggc
     8281 tatcgcgcct atacgacgcg ccatgaccgc gagcttgttg ccggcacgct ggtccgcaaa
     8341 gcattgctcg atcagtttcg cgagcagctc gacgagcgcg tcgccgtcca ggggatcaac
     8401 gtggcgcggc ttgcgcacgc actgaaaacg gcgctggcga taccgcaacg cgatggatgg
     8461 tcgttcggtg aggaacatgg ccggatcgac ggtcggcgcc tcgcgcaact ggttagttct
     8521 cccgctgaac ggcgcctgtt ccggctggag cggaacacgc tcagggcgga ctgcgtggtc
     8581 ggcttcctga tcgactgctc gggctcgatg aaagcgcata tcgaaccggt ggcgatcatg
     8641 gtggatatcc tcacccgcgc gctcgaccag gccggcgtga ccaccgaggt actcggcttc
     8701 accaccggcg cctggaacgg tggccgagcc cggctcgact ggctggcgca gggccggccg
     8761 cctcatccgg ggcggctcaa cgaactgtct catctggtgt tcaaggacgc cgggcgcagc
     8821 tggcgacgcg cccgcgccga tatcgcggcg ctcttcaagg cagacctgtt tcgcgagggc
     8881 gtggatggcg aggcggtcga ctgggcttgt gggcgcctcg tcgcgcgttc tgaagcgcgg
     8941 cgcattctga tcgtgatctc ggatggcagc ccgatggaca gcgcgacggg gcaggccaac
     9001 gacgccttct atctcgacaa ccatctgaag gccgtcgtgg cgcgtcacga tgccttgcgc
     9061 gacgtcgaag tgctgggttt gggcgtcggg ttggatctca gcccgtatta ccgccgatgt
     9121 ctcgccgtgg atctctccgt gccgccggat atgaagctgt tcgcggagat cgtcgggtgg
     9181 attggtgcgc gtgggcgggg cggaaaagat tagcggggga gtggaaaagg gtagcgggtt
     9241 ggagcggctt gctgctttat attcgcggct tcacgtccgc ggcttcacat tcactactta
     9301 ggcccacggc ttgacattca ccgctcaagt ccacggcttc acgtgtatct tgcggcgtct
     9361 gtcaatgcga gctgacgccg catcttccca atgctcatga agcatggtgc ggcatgcgat
     9421 gcgcgcttac cggctcatct gcggcttgtg cctgcacagc cgtcaccgcg atcacatagt
     9481 agacatcgtc cgcactgcag ccgcgcgaaa gatcgttggc cggtttgttc agaccttgca
     9541 acaaggggcc gatggccttc gcatcaccga tccgctcagc cagcttgtag ccgatattgc
     9601 cggcctccag gctcgggaaa atcagcacgt tggcatgccc ctgcacctgc gaatgatcga
     9661 ttttgcgcag ggcgatttcg gagacgaggg ccgcgtccag ttgcacgtcg ccgtcgatcg
     9721 ccagatgcgg gcgcgcggct ctcacctgac gcgttgcttc caccaccttg tcgaccgccg
     9781 cgtgccgggc gctgccgctg gtcgagaacg acagcatggc cacgcgcggc gcctccatca
     9841 gcaggctttg cgcgctgtcg gccgccgcca ttgcgatctg ggcgagttca cccgcgttcg
     9901 ggtccaccac cagtccgcaa tcggaaaaga tcaggccgcc tttgagggtg tggaagggct
     9961 cgcacagcat catcagaaag aagctcgaca ccagcttgaa cgatggatgg ataccgatga
    10021 tctggatcgc cgtgcgaacc acgtcggcag tcgtgtggac gcagccggcc accgaaccgt
    10081 cggcatggcc gagacgcacc atcaggttcg cgaagcagag gggcttgagg acttcctgtt
    10141 tcgcttcttc cagcgtcatg ccttttttgc tgcgcagggc gaagagtgcc tcggaaaacg
    10201 atggggtgaa gagcgacgtc gcggggtcca ccagctccat gccggccagc tcgatgttct
    10261 gcccggcggc cgctgcacga atgcgcgcgg ccggacccac cagcacgatg cgggcgatgc
    10321 cttcacgcgt ggcgcggtgc gctgcctgca acatgcgggg atcttccgct tcgcagagca
    10381 cgatgcgtct gggcgaggtg cgggcgcgtt caatgatgcg gttgatggct ttcatgggct
    10441 agggcggcgc tcacggttga taaaacggaa acaacgaacg gcaaacaacg aacggcaaac
    10501 acaaacggca aacagcaaaa caacgaaaca ggccggaaac ggcaggcgac actccgcttc
    10561 cggcccgccg ggcgcgagga gatgtcctcc cgcgcccgga ccctcagaca acggaccgct
    10621 cagacataat ccttatactt gtccagcaag cgcacgggct tggccagcgc gtcgcgacgg
    10681 aacggatcgc cgagctcgcg ggtacacatg atctcgatga tggtggtttt gccgtggttc
    10741 atctgtgcat cgatcgcacg ctttaacgcc gggccgacgt cctcgagccg atccaccgtg
    10801 acaccttcgg cacccatcgc gcgcgcgata ccggcgaagc tctgattgtc cagttcaccc
    10861 gccacgaagc ggcggttata gaagtccacc tggttcttct tctccgcgcc ccactgccgg
    10921 ttatggaaca ccaccgcggt gaccggaatg ttgtgacgca cgcaggtcat ggtttccatc
    10981 aggctcatgc cccacgcacc gtcaccggcg taggagacag cgggacgatg gggcgccgca
    11041 accttggcgc cgatgatggt ggggaacgcg tagccgcagt tgccccagct catcgcggcg
    11101 aagaagctgc gcggcttggt aaagcgcaag tagctgttcg cgacggagtt gatgttgccg
    11161 atgtcggtgg agaccatcac gtcgtccggc atggcctttt cgagctcgcg caatacctgg
    11221 cgcggatgca gatagtggcc gccgccgggc gtgcgctcgt ttttctgctc ttcgatcatg
    11281 tccatgctgt aggggtcgcg ctcgtgggtc caaccgtcga gctccttctc ccatgcagcc
    11341 ttctccgcag cgatgtggcc ggcgcggtct tcacgcgagg cgtcgcaagc gagcgtgcgg
    11401 ccttcgagac gctgtgtgag cgcaaccgcg gcggccttgg cgtcgccgca gatgcccacc
    11461 gagatcttct tcaccagacc gagcatcttg tggtcggcat cgatctggat gatctttgcg
    11521 ttctgcggcc agtagtccat gccgtgctgc ggcagcgtgc cgaacggtcc cagacgcgaa
    11581 ccgagcgcga ccaccacgtc ggcgcgctgg atcagcttca tcgccgcctt ggagccctga
    11641 tagccgagcg ggccgcacca cagcggatgg tcggcgggga aggagtcgtt atgcaggtag
    11701 ctgttgacga ccggcgcacc gagacgctca gccagtgcct tgcattcctc gatcgcatcg
    11761 gccatgacca cgccgccgcc cgagatgatg accgggaatt tggcctgggc gagcagttcg
    11821 gccgcttcat tcaaacgttg gtcgccgccg gggccgcggt cgaggcgctg cggctgcgga
    11881 atctcggcct tgatctggcc atagaaataa tcgcgcggaa tgttgagctg ggtcgggccc
    11941 atttcggcca gcgcgcggtc gaagcagcgg ccggtgaatt cagccatgcg cgcggggtgg
    12001 gtgacgtggc cctgatactt ggtgaactcc tggaacatgg gcagttgctt cgcttcctgg
    12061 aagccgccca gaccgatgcc catggtgccc gcctcgggcg tgatcatgac caccgggctg
    12121 tgcgcccaat aggccgccgc gatggcggtc acgcagttgc tgatgccggg gccgttctgg
    12181 ccgatcacca cgccatggcg gcccgataca cgtgcatagc cgtcggccat gtggccggcg
    12241 ccctgttcat gcaccacggg gatgaggcgg atgccggcgg gcgcgaagat gtccatggcg
    12301 tccatgaagg ccgaacccat gatgccgaac atgtcggtca cgccgttcgc ggccagggtt
    12361 tcaacgaacg cttccgaagg ggtcatggcc tgcgggccgc tgacaggggt gtgatcgctc
    12421 atgagggctg tctccgatgt ttaatgaaac cggaacaaat cgttccgaaa agtggttatc
    12481 taaaatgtag accatgcatc tggcacggtc aataggtgat gaaagataat cggtacaatt
    12541 tgtctcgaaa attaatcatt gcgatgctgt ggctgtggct gtggctgtgg ctgtggctgt
    12601 ggctgtggct gtggctgcgc gcgcggctca atcccacggc agcgaatagg tcttcacatt
    12661 ggtgaagctt ttcatcgctt cctggacgcc ttccttgtag ccaagacccg aatccttgat
    12721 gccgccgaac ggcgtgagtt cgagccggta acccggcact tcgcgcacgt tcacgctgcc
    12781 cacctccagt tcggcgataa agcgcgtgat gtagtcgagg cgattagtgc agaccgaaga
    12841 cgaaagcccg tagtcggtcg aattggaaat ccggatcgcg tcgtcgatgt cgcgaaagcg
    12901 gatgaccggc gacacgggcc cgaaggtttc gtatttgacg agcggcatgt ccgggctcac
    12961 gtgatcgagc accgtcggcg aatacagcgc gccctcgcgg cggttgccga gcagcaaggt
    13021 cgcgccacgg ctgaccgcat cgttgacctg tgcttcgaag aacttcgctg cggcttcgtc
    13081 gatcacggtg cccatgtcgg ttgccggatc ggcgggatcg ccataacgta tggcccggct
    13141 cttctccacg agcagttcga cgaagcggtc ggccaccgcc tcatgcacga gaattcgctt
    13201 gatcgccgta caacgctggc cggagttctt gtaggagccg gagacggcga gcgtgctggc
    13261 ttcttccagg tcggcgtctt ccatcacgat gattggatcg ttgccgccga gttcgagcac
    13321 cgcgcgccga tagcccatct tgctggcgat gctcttgcca atctgcacac cgccggtgaa
    13381 ggtgatcaga tcgacgtgct cgttcgtgat cagttcgtcg gcgatgtctt tcgggtcgcc
    13441 ggtaatgact tgcagcattt cctgcggcaa gccggccgcg taaaggatgt ccgcgagcag
    13501 atacgtggac agcggcacct tttcagacgg tttgacgacg atccggttgt tggtcgcgat
    13561 ggacggcacg actttgtgcg caacctgatt catcggatgg ttaaacggcg tgatcgcgga
    13621 gatgacgccg agcaacggat cgcgctgcgt gtacacgcgg cgcttcttgc cgtgcggcgt
    13681 gagatcgcag gaaaacacct ggccgtcgtc cttcagcgct tcgttggcgc cgaatgtcat
    13741 gacgtcggcg acacggcctg cttcgtagag cgagtctttc ttgcacaggc ctgattcggc
    13801 ggtgatcaga ccggcgattt cctcagtacg ccgacgcact ccatctgctg cgcgcagtag
    13861 gatctgtgag cgctcgtagc gtgtgaggcg agcgcgatac tggtgtgccc aggcgaaggc
    13921 gcgtcgcacg tcggcgagcg tcgccttggg caccgtgccg accagttcgt tggtataggg
    13981 attgaagact tcgatgatct ccgcgcgccg gatgagttct ccgccgatgc gcagcgcttc
    14041 atgacgaacc tgcggcgcgg gccagacttt cgcgatt
//
LOCUS       DATASH010000357         4103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2819796,
            whole genome shotgun sequence.
ACCESSION   DATASH010000357 DATASH010000000
VERSION     DATASH010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2819796"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1091)
                     /locus_tag="VHV99_10095"
     CDS             complement(<1..1091)
                     /locus_tag="VHV99_10095"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017423898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HEX3635824.1"
                     /translation="MTPLTPLTIRGRTLLPVVQGGMGVGVSAHRLAGAVAREGAIGTI
                     SSIDLRHHHPDLLEMCKQNPGQATLEAANQTALRREIIAAKALGEGRGMIAVNVMRAV
                     SSYAAAVQTACESGADAIVMGAGLPLDLPDLTQGVDIALIPILSDSRGIALVLKKWLK
                     KNRLPDAIVIEHPGHAGGHLGVASVADIPSERFDFARVIEETMLTFDTLGIGRTQIPL
                     IVAGGIDGHAAVRRWLAAGASGVQLGTAFAVTEEGDAHPEFKRILADATPEDIVEFVS
                     VTGLPARAVKTPWLDRYMRNEQRIRDKIGARAQKCPTGLNCLVACGLRDGIEKFGHFC
                     IDTRLAAALRGDRANGLFFRGREALPFGSA"
     gene            complement(1314..2906)
                     /locus_tag="VHV99_10100"
     CDS             complement(1314..2906)
                     /locus_tag="VHV99_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HEX3635825.1"
                     /translation="MLSTANITMQFGPKPLFENISVKFGEGNRYGLIGANGCGKSTFM
                     KILGSDLEPSSGNVMLEPNIRLGKLSQDQFAYEEKRVIDVVMIGHTEMWRAMSERDAI
                     YANPDATDDDYMHAAELEAKFAEYDGYTAEARAGELLLGIGIAIEDHNGPMSNVAPGW
                     KLRVLLAQALFSKPDVLLLDEPTNNLDITSIRWLEDILNQYNSTMVIISHDRHFLNQV
                     CTHMADMDFGTLKVYPGNYDDYMLASAQARERQASANQKAKDKVADLQDFVRRFSANK
                     SKARQATSRAKQIEKIKIEEFKPSSRQNPFIRFDFEKKLHNIAVVATNITKKYDRTIF
                     NHFSLSVQPGERIAIIGENGAGKTTLLRALKGAIELDGGTVKWAENALVGYMPQDTYE
                     EFPKDETLTDWVDQYRQDGDDDQMVRGTLGRLLFNADDIKKSVKVLSGGEKGRMIWGK
                     LMLGRPNVLLMDEPTNHMDMESIESLQIALEKYDGTLIFVSHDREFVNGLANRIIEVK
                     NDGTLRDFGGTYEDFLTSQGIH"
     gene            complement(3161..>4103)
                     /locus_tag="VHV99_10105"
     CDS             complement(3161..>4103)
                     /locus_tag="VHV99_10105"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007749928.1"
                     /note="catalyzes the formation of L-threonine from
                     O-phospho-L-homoserine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX3635826.1"
                     /translation="KQGVRVFMLSPHQKMSAFQTAQMFSLQDPNIFNLAVIGNFDNCQ
                     DIVNAVSNDHAFKAANKIGTVNSINWARVVAQVVYYFKGYFAATKSNAERVSFTVPSG
                     NFGNVCAGHIARMMGLPIEQLVVATNENDVLDEFFRTGVYRVRTAAETYHTTSPSMDI
                     SKASNFERFVFDLLGRDPSRVLQLFRDVEEKGGFDLAAGGDFVRVKEFGFVSGRSSHA
                     DRVDTIRDAFKRYGTMIDTHTADGLKVAREHLRPGVPMIVLETAQPIKFGETISEALE
                     REPERPAAFIGIEELPQKFDIVAAEAGLVKAYIAERI"
BASE COUNT          784 a         1291 c         1156 g          872 t
ORIGIN      
        1 gctgatccga acggcagcgc ctcacgtccc cggaagaaca atccgttcgc gcgatctcca
       61 cgcagggccg cggccagacg ggtatcaata caaaagtgcc cgaacttttc aataccatcg
      121 cgcaaaccac acgccaccag gcaattcaga cccgtggggc acttttgcgc acgcgcgccg
      181 atcttgtcgc gaatccgttg ctcattgcgc atgtaacgat cgagccacgg tgttttcacc
      241 gcgcgagccg gcaggccggt cacactcacg aattcgacaa tgtcctccgg cgttgcgtcg
      301 gcgagaatgc gcttgaactc aggatgcgca tcaccttctt ccgtcaccgc gaacgccgtg
      361 ccgagttgca cgcccgacgc acctgccgca agccagcgcc gcaccgccgc atgcccgtcg
      421 ataccacccg ccacgatcag cggaatctgc gtacggccga tgccgagggt atcgaaggtc
      481 agcatcgttt cctcgatcac acgggcgaag tcaaagcgct cgctcggaat atccgcgacg
      541 ctggccacgc caagatggcc acccgcatgc cccggatgct cgatcacgat ggcatcgggc
      601 agacgattct tcttgagcca cttcttcagt accagcgcga tgccacggct atccgacaag
      661 atcgggatca gcgcgatatc aacgccttgc gtcaggtcgg gcaggtccag aggcagtcct
      721 gcgcccatga cgatagcgtc ggcaccgctc tcgcacgccg tttgcaccgc tgccgcgtag
      781 gacgacacag cgcgcatgac attcacggca atcatgccgc ggccctcgcc aagcgccttc
      841 gctgcaatga tctcgcggcg caacgccgtc tggttggcgg cttccagcgt cgcctggccc
      901 ggattctgtt tgcacatttc aagcaggtcg ggatggtgat gccgcaagtc gatgctcgag
      961 atcgttccga ttgcaccctc ccgggcgacg gcgccggcca gccggtgcgc ggacacaccc
     1021 acgcccatgc ctccctgcac aacaggaagc aaggtgcggc cgcgaatggt caggggggta
     1081 agaggggtca tgaaagggtc atctccagag tcaaagtttt gagcttgcgg gaggttggac
     1141 agattgatgt tgctttttat caagcaagtt cgactgtgct gtatttcagt ttccgaacgt
     1201 gttccgttcc ccgaaaacgc aaacggcccg cttcgtgacg aagcgggccg tttgcgtggc
     1261 tacctgtttc taatcgctga ctgcggtgtt acatgggatg taacgcggcc aggttaatga
     1321 ataccctggc tcgtcaggaa gtcctcgtac gtaccgccga aatcgcgcaa cgtgccgtcg
     1381 ttcttcactt cgatgatccg gttcgccagt ccattgacga attcccggtc atgcgaaacg
     1441 aagatcagcg tgccgtcgta tttctccaac gcgatctgca gggactcgat tgattccatg
     1501 tccatgtggt tggtcggctc gtccatcaac agcacattcg ggcgcccgag catcagcttg
     1561 ccccagatca tgcggccttt ctcgccaccc gagagcacct tgaccgactt cttgatgtcg
     1621 tcggcgttga acagcaaacg gcccaacgtg ccgcggacca tctggtcgtc gtcgccgtcc
     1681 tggcgatact ggtcgaccca gtcggtcagc gtctcgtcct tcggaaactc ttcgtacgtg
     1741 tcctgcggca tatagccaac cagcgcgttt tccgcccact tgacggtgcc gccgtccagc
     1801 tcgattgcgc ccttcagcgc gcgcagcaac gtggtcttgc ccgcgccgtt ctcgccgatg
     1861 atcgcaatcc gctcacccgg ctgaacgctc aggctgaagt gattgaagat ggtgcggtcg
     1921 tatttcttgg tgatgttggt cgccaccacc gcgatgttgt gcagcttttt ctcgaagtcg
     1981 aagcgaataa acggattttg ccgcgacgac ggcttgaatt cctcgatctt gatcttttcg
     2041 atctgcttcg cacggctggt cgcctggcgc gccttcgact tgttggccga gaaccggcgc
     2101 acgaagtcct gaaggtcggc tactttgtct ttcgccttct ggttcgcact cgcctgacgc
     2161 tcgcgcgcct gggccgaagc cagcatgtag tcgtcgtagt tacccgggta caccttcaac
     2221 gtgccgaaat ccatgtccgc catatgcgtg cagacctggt tcagaaagtg tcgatcatgc
     2281 gaaatgatca ccatggtcga gttgtactga ttcagtatgt cctcgagcca gcgaatggag
     2341 gtgatgtcca ggttgttggt cggttcgtcg agcagcaaca catccggctt cgaaaacagt
     2401 gcctgcgcga gcaagacgcg cagtttccag cccggggcaa cgttgctcat gggaccgtta
     2461 tggtcctcaa tcgcaatgcc aatgcccagc aacaactcgc ccgcgcgcgc ttcggccgta
     2521 taaccgtcgt actcggcgaa cttcgcttcg agttcggcgg cgtgcatgta gtcgtcgtcg
     2581 gtcgcgtcgg gattcgcgta gatggcatcg cgctcggaca ttgcgcgcca catttccgtg
     2641 tggccaatca tcacgacgtc gatcacgcgc ttttcttcgt aggcgaactg atcctggctc
     2701 aatttgccca accggatgtt cggctccagc atgacgttgc ctgaactcgg ctcaaggtcg
     2761 ctgccgagga ttttcatgaa cgttgatttg ccgcagccgt tcgccccgat caggccgtag
     2821 cgatttcctt ccccgaactt gaccgaaatg ttctcgaaga ggggcttcgg cccgaattgc
     2881 atggtgatgt tggcagtaga cagcacagcg cgtccttttg aatggtgtac ggcgaaaaac
     2941 ctgcaatttt agcagttttt gcgacgaatt gcggcattgc agcgaaaagc cgcacgcgcg
     3001 agtgcgtaga ccgcacttcc ggcggtcgat aatcgtgccg caaacggcgc cgcgagtgac
     3061 gccgcacatg acgccgcgca tgccgccgcg catgccgccg cgccgggcgt tccgggctga
     3121 ctgaatctgc ccgaatccgc tggcgccgcc ggtcccgagc ctagatgcgc tccgcgatat
     3181 acgccttcac gagacccgcc tcggccgcca cgatatcgaa tttctgtggc aattcttcga
     3241 tacctataaa cgccgccggc cgctccggtt cgcgctcaag cgcttcgctg atggtttcgc
     3301 cgaatttgat cggctgagca gtttccagca cgatcatcgg cacgcccggt ctcaggtgct
     3361 cgcgcgcaac cttcagaccg tcggcagtgt gggtatcgat catggtgccg taacgcttga
     3421 acgcgtcgcg aatggtgtcc acccggtccg catggctgct gcgtcccgac acgaaaccaa
     3481 actccttcac gcgcacaaag tcgccgccgg ccgcgaggtc aaatccacct ttttcttcca
     3541 cgtcccgaaa gagctgcaat acgcgcgagg gatctcggcc gagcagatcg aacacgaaac
     3601 gctcgaaatt cgatgctttc gagatatcca tgctcgggct ggtcgtgtgg tacgtctcgg
     3661 ccgccgtgcg cacgcgataa accccggtgc ggaaaaattc gtcgagcacg tcgttttcat
     3721 tcgtggcgac aaccagttgc tcgatcggca accccatcat gcgcgcgata tgtcccgcgc
     3781 agacattgcc gaagttgccc gatggcaccg tgaacgacac ccgctcggcg ttcgacttcg
     3841 tcgcggcgaa gtagccttta aaatagtaga cgacctgagc gaccacacgt gcccagttga
     3901 tcgaattgac cgtgccgatc ttgttggccg ctttgaacgc gtgatcgttc gacaccgcat
     3961 tgacgatgtc ctggcaattg tcgaagttgc cgatcaccgc cagattgaag atgttcgggt
     4021 cctgcagcga aaacatctgc gccgtctgga acgcgctcat tttctgatgc ggcgacagca
     4081 tgaaaacgcg cacgccctgc ttg
//
LOCUS       DATASH010000358         2773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4205636,
            whole genome shotgun sequence.
ACCESSION   DATASH010000358 DATASH010000000
VERSION     DATASH010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4205636"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VHV99_10110"
     CDS             complement(<1..279)
                     /locus_tag="VHV99_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635827.1"
                     /translation="MERVIGAVLLRIFLWLADSALADTPGQEESIGPQACGAFQRDTV
                     PHSIRSAEFIDEHIESVPPQEQVYLQTESSVIEFGPDTGSHFVFLMRRP"
     gene            complement(477..596)
                     /locus_tag="VHV99_10115"
                     /pseudo
     CDS             complement(477..596)
                     /locus_tag="VHV99_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007738055.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            955..2445
                     /locus_tag="VHV99_10120"
     CDS             955..2445
                     /locus_tag="VHV99_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3635828.1"
                     /translation="MSTTYDCAWLLADPGTQEAQANMRLVVEAGRIAAIAHAPELSPG
                     PRRLVLPALTNAHDHGRYFRSATVDAFEKPLESWLPFMGLIPGADPYLSAATAFARSV
                     RNGIANLMVHYTRVQGGMPYVVEARAVARAARDVGNRIGFAIAMRDCHGIAYCDDATA
                     LVALRPEIRDAVAARLSTKPLLPAQQLALVEEVARMVEEESYAEHVTVQYGPTGVQWC
                     STRLLEAIATASADHDRPIHMHFLETRYQREWADQTHPEGIVRYLDDLGMLSPRLTLA
                     HCVWARPEELSLLAERGVTIAVNTSSNLYLKSGIAPVPDMLRHGCRIALGLDCTGIDE
                     DNDALREMRLAYQLHRGWGFEVNMNRAQLWTFAAQNGRHSVNGAACDASLGGRLAVGA
                     PADLVLLNWDALDDDGLLPVDPLDLLLARGSGMHIDEVVIGGRTVVAQGRACGVDEHA
                     LRGELIAQMRERIAAQPAVGAWHGTLRALAEDLGPFYLKSAWRGCC"
     gene            complement(2417..>2773)
                     /locus_tag="VHV99_10125"
     CDS             complement(2417..>2773)
                     /locus_tag="VHV99_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3635829.1"
                     /translation="SPDEPIRNLSGGNVQRAILSRELSGDVKLLIVANPCFGLDFASA
                     AEIRAQIIEQRNRGAAVLLISEDLDEITELADRIAVMSEGRIVYDAPAQEADQQSIAR
                     AMAGEAQISSSPSTRS"
BASE COUNT          531 a          853 c          843 g          546 t
ORIGIN      
        1 aggccgccgc atcaaaaaca cgaagtgact cccggtatca ggcccgaact caatgactga
       61 gctttccgtt tgcagataca cctgttcctg tggcggcact gactcgatgt gctcatcgat
      121 gaattcggca ctgcgaatac tgtgcgggac ggtatcacgc tggaacgcgc cgcaagcctg
      181 tggtccaatg ctttcctctt gtccaggcgt gtcagccaga gcgctgtcag ctagccacag
      241 aaatattctc agcagcactg ccccgattac cctctccata cacccctcct gtgctgtttg
      301 ttgctccgcg cacctttgcg taggcaaacg agcgtctcgc tgacgcgcaa tcaattcgct
      361 agagccggtg cataagtctc atggcgaggc tatcgcttat ttagccaggt tcggttcaac
      421 tcggcattgc ttatgataag gtgggcaagg tgaagaaatt tatatgataa ggattcctac
      481 atcatcagac aatagaactg ttcagcggct gttcgcgaga cgccatcgtc cttcatctga
      541 gctttgcgga tcatgtgcat cgtctcagtg cctgagagga tgatgcgagc acaccgctgt
      601 accgggtagc cttgggtgac cgtcgtagag cgattcgatt tcttcatcgc gtcatcttac
      661 gcgaccgctc ccgcaacttg gcactgcccc gttacgtcca cgtccagtcg agccgcgtag
      721 tctcaccgtc atcgaaggcg agatcttccg caagccgatg ccgtaaaatg accaaaacgt
      781 ggttgcgaac tccagcaaaa gatcgcattc ttgatgccct ccgaagccgc gtttcggggc
      841 tattgcgagt gattttttta cgccgcttat ccagggccac tcacggcgat cggtaacacg
      901 cgtcactagt agttcgcgat aagcgccaac aaatccatcg aagaagattt tcacatgagc
      961 acaacctatg actgcgcatg gctcctggcc gaccccggaa cccaggaagc gcaagccaac
     1021 atgcgcttgg tagtcgaggc aggccgcatc gcagctattg cacacgcgcc cgaactgtcg
     1081 cccggcccac gccgcctggt cttgccggca ttgacgaacg cgcacgatca cggtcggtac
     1141 ttccggagcg cgaccgtcga tgcattcgaa aaaccgctgg agagttggct gcctttcatg
     1201 ggactcatcc ccggtgctga tccttacctc agtgcagcga cggctttcgc acgttcagtg
     1261 cgcaacggca tcgcaaacct gatggttcac tacacgcgcg tgcaaggtgg catgccctac
     1321 gtcgtcgagg ctcgagctgt ggcccgggct gcgcgcgacg tcggtaaccg catcggcttc
     1381 gccatcgcga tgcgcgactg ccacggcatc gcttactgcg acgacgcgac agcactggtt
     1441 gcgcttcgcc cggagatccg ggacgcggtc gcggcgcgcc tgagcaccaa accgctgctg
     1501 ccggcgcagc agctggcact cgtcgaagag gtcgcccgca tggtcgagga ggaaagctac
     1561 gccgaacacg tgaccgtgca atacggtccc acgggcgtgc aatggtgctc cacgcggctg
     1621 ctcgaagcga tcgcgacggc atcggccgac cacgaccggc cgatccacat gcatttcctc
     1681 gagacgcgct accagcggga atgggctgat cagacgcatc cggaaggaat cgtcagatat
     1741 ctcgacgacc tcggcatgct cagtccgcgg ctgactttgg cgcactgcgt ttgggcccga
     1801 cccgaggagc tttctctgct ggccgagcgc ggtgtgacga ttgccgtgaa cacgagctcg
     1861 aatctgtacc tgaagtccgg gatcgcaccg gtgccggaca tgctgcggca cggttgccgc
     1921 atcgcgctag gcctggattg caccggtatc gacgaggaca acgacgcgct acgggagatg
     1981 cgcctggctt accagctgca ccgcggttgg ggcttcgaag tgaatatgaa ccgtgcacag
     2041 ctttggacct tcgcggcgca gaacggccgc cacagcgtga atggggcggc ctgtgacgcg
     2101 tctttgggcg gtcgtctggc cgtaggagcg ccggccgacc tggttctgct gaactgggac
     2161 gcgctcgacg acgatggcct gctgccggtc gatccgctcg acctgttgct ggcgcgcggt
     2221 agtggcatgc acatcgacga ggtcgtcatt ggaggcagga ctgtggttgc acagggccgg
     2281 gcatgcggcg tcgacgaaca cgcgctgcgc ggcgaactga tcgcacagat gcgcgagcgc
     2341 atcgccgcgc agccggcagt gggcgcttgg catggcacgc tgcgcgcgct ggccgaggat
     2401 ctgggaccgt tctacctcaa gagcgcgtgg aggggctgct gctgatttgc gcctccccag
     2461 ccatcgctcg tgcgatcgat tgctggtcgg cctcttgcgc cggcgcgtca tacacgatac
     2521 gtccttcgct catcaccgcg atgcggtcgg cgagctcggt gatctcatcg aggtcttcgg
     2581 aaatcagcag cacggcggca ccgcgattgc gctgctcgat gatctgcgcg cgaatctcgg
     2641 cggccgatgc aaagtcaagg ccgaaacaag ggttcgcgac gatcaagagc ttgacgtcac
     2701 cggacagctc acgagaaagt atcgcgcgct gcacgttgcc gccggagagg ttcctgatgg
     2761 gttcgtcggg cga
//
LOCUS       DATASH010000359         5020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5788416,
            whole genome shotgun sequence.
ACCESSION   DATASH010000359 DATASH010000000
VERSION     DATASH010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5020
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5788416"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(206..1543)
                     /gene="der"
                     /locus_tag="VHV99_10130"
     CDS             complement(206..1543)
                     /gene="der"
                     /locus_tag="VHV99_10130"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEX3635830.1"
                     /translation="MKPVIALVGRPNVGKSTLFNRLTRTRDALVADLPGLTRDRHYGE
                     GRAGDRPYLVVDTGGFEPVAKDGILHEMARQTRQAVEESDVVVFIVDGRNGLAPQDKT
                     IADYLRKVGRPIFLVVNKAEGMKYSTVAADFYELGLGDPRAISAAHGDGVTEMINEAL
                     GVAYAGQPEEEEGEKEARGVKIAIVGRPNVGKSTLINALVGEERVIAFDMPGTTRDSI
                     YVDFERQGKPYTLIDTAGLRRRGKVFEAIEKFSVVKTLQSISDANVVILLLDARQDIS
                     EQDAHIAGFVVEQGRALVVGVNKWDGLDPHVRERTKADLERKLKFLDFAKFHFISAAE
                     KTGIGPLMRSVDDAYAAAMAKLPTPKLTRALIDAVEFQQPRRRGPVRPKLRYAHQGGQ
                     NPPIIVIHGNALDAITETYKRYLENRFRETFKLTGTPLRIEFRSSTNPYADKG"
     gene            complement(1952..3097)
                     /gene="bamB"
                     /locus_tag="VHV99_10135"
     CDS             complement(1952..3097)
                     /gene="bamB"
                     /locus_tag="VHV99_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HEX3635831.1"
                     /translation="MNLLKRYAVPVACAMTVLTMAACSSTKDERRVPTLLTEFKPVLD
                     VQQAWKASVGKAGRYLFSPVAVGNAVYAAGANGSVAKIDAQTGQDIWRVKLHDDLSAG
                     VGSDGTLTAVGGLKGDVYVLGADGKQLWTAKAPGEIISPPLVGNGLVVVRTVDGQIVA
                     FNAQTGEQKWNYRNRAVPLNLRVSSGMTFAGDAAVLAGFPGGSFAAINLQTGDNYWQT
                     PVSYPKGVTEVERINDVTGPPTLVGSETCAVTFQGQIGCFDANSGRAVWEKAFSSTSG
                     LAQDDRAVVAADDWSVVSAFDVSNGAPLWKNDKLKNRELSVPFVLGHAAVLGDYQGYV
                     HFLSRDDGTLVARVKTDGSAITAAPVLAGETLVVQTHDGDLYGYRPR"
     gene            complement(3173..3802)
                     /locus_tag="VHV99_10140"
     CDS             complement(3173..3802)
                     /locus_tag="VHV99_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3635832.1"
                     /translation="MSYHDEQESIESLKAWWTQWGNATTWIVLVALVAAAGWNGWNFW
                     QRRQSTEAAVLYDQVQQAVASGDKAKITRVATDMEDKFSRTAYAQMTALGAAKALYAA
                     GDEAAAKAQLQWTIDHAKDDEFKQIAKLRLASLLLDDKAYDQGLALLAEPQSDAFKGI
                     VANGRGDLLAAQGKRDDARAAYKLALDTLSKNDSSARQLIQFKLDALGG"
     gene            complement(3869..>5020)
                     /gene="hisS"
                     /locus_tag="VHV99_10145"
     CDS             complement(3869..>5020)
                     /gene="hisS"
                     /locus_tag="VHV99_10145"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006764216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEX3635833.1"
                     /translation="EVTDIVEKEMYSFTDALNGENLTMRPENTAAVVRATIEHNMLYD
                     GPKRLWYIGPMFRHERPQRGRYRQFHQVGVEALGFAGPDTDAEIIMMCQRLWDDLGLM
                     GIKLELNSLGLVEERAAHRVELIAYLEKHMDVLDEEAKRRLYANPLRVLDTKNPAMQD
                     VAQNAPKLIDFLGEESRAHFEGLQRILKANNIPFTINPRLVRGLDYYNLTVFEWVTDK
                     LGAQGTVAAGGRYDPLIEQLGGKPTGACGWAMGVERILELLKEEQLVPEDEGCDVYVV
                     HQGDAAREQAFIIAERLRDTGLDVILHCSADGQTASFKSQMKRADASGAAFAVILGED
                     EIANGTVGVKPLRDTGANGGKNEQHNVPAEDLTEFLINAMVATADDGDD"
BASE COUNT          885 a         1632 c         1483 g         1020 t
ORIGIN      
        1 tacgcagtgc gttcaaaaac gggtcttgta acaattgccc tttgttgctc atagcaaact
       61 ccgtattttt ttgcaggttg actgaattga cgacgaagaa aaagagatcc gccgtcaacc
      121 gctacactat agccgatttt catttctgcg cgcgggcccg gcctggcggc caaacccagc
      181 acacacgcgg gttttcagcc tgggtttagc ctttgtccgc gtaagggttc gtcgacgatc
      241 tgaactctat gcgcaatgga gtcccagtca gcttgaaagt ttccctgaag cggttttcga
      301 ggtagcgttt gtacgtttcg gtgatcgcat cgagcgcgtt accgtgaatc acgatgatcg
      361 gcggattctg tccgccctgg tgcgcgtaac gcagtttcgg acgcaccggg ccacgtcggc
      421 gcggctgctg aaattcgact gcatcgatta gcgcgcgcgt cagcttcggc gtcggcagct
      481 tggccatggc cgccgcgtaa gcgtcgtcga ccgaacgcat cagcgggcca attccggttt
      541 tttccgcagc ggaaatgaaa tggaatttgg cgaagtcgag aaattttagt ttgcgctcca
      601 ggtccgcctt cgtgcgctcg cgcacatgcg gatcgagacc gtcccacttg ttcacgccca
      661 cgaccagcgc acggccctgc tccaccacga agccggcgat gtgcgcgtcc tgctccgaga
      721 tatcctggcg tgcgtcgagc agcaggatca cgacgttcgc gtcagaaatc gactgcagcg
      781 ttttcaccac cgagaatttc tcgatcgctt cgaacacctt gccgcggcga cgcaaacccg
      841 ctgtgtcgat cagcgtgtac ggcttgccct gacgttcgaa atcgacgtag atcgaatcgc
      901 gcgtggtgcc tggcatgtcg aacgcgatca cgcgctcttc gccgaccaac gcgttgatca
      961 aggtcgactt gccaacgttc ggccggccga caatggcaat cttcacgccg cgcgcttctt
     1021 tctcgccttc ttcctcttcc ggttgccccg cgtacgcaac gccgagcgcc tcgttgatca
     1081 tttcggtgac gccgtcgccg tgcgccgccg agatcgcgcg cgggtcgccg agaccgagtt
     1141 cgtagaagtc ggcggccacc gtgctgtact tcatcccctc cgccttgttg acgacgagga
     1201 aaatcggccg accgaccttg cgcaagtagt cagcgatcgt cttgtcttgc ggcgcgagac
     1261 cattgcggcc gtcgacgatg aacacgacga cgtccgactc ctccaccgcc tggcgggttt
     1321 gacgcgccat ctcgtgcagg atgccgtcct tggcgaccgg ctcgaagccg ccggtatcga
     1381 cgaccagata cggccggtcg ccggcgcgcc cttcgccgta atggcgatcg cgggtgagac
     1441 cgggcaggtc ggcaaccagc gcatcacgcg tgcgcgtgag acggttgaat agcgtggatt
     1501 tccccacatt ggggcgccca accagggcaa taacgggttt catcagatgt tgttcacagt
     1561 gaaacgcggg atcgaaatcg accgcccgcg tagcgacgcc atgcggccgc tacacggcag
     1621 cgtttgacga aaattatcac gaattcggcc agccccggat gcgctgtcaa tatgagcacc
     1681 cgggcggtcg gggggttgac tgtatggggc ggcgccagtg ggccgccagg ttcttcagtc
     1741 tttcttgctc aacgactcgc acgccagttc aatgcgattg ccgtggaagg tggcgccgca
     1801 agcagccacc tatggccaca tgggccgttt ctggtccgac ttgccggacc agccagtccg
     1861 gcggaagtct tctagttgcc ctgctgtaca gcaaaaacgc gcgcggccgg cgaagccggc
     1921 ctgcacgctc gactacccaa ggcctctgcg attagcgcgg gcggtagccg tacaggtcgc
     1981 cgtcgtgcgt ctgcacgacc agtgtctcgc cagccagcac cggtgctgcg gtaatcgcgc
     2041 tgccgtcggt cttgacgcgg gccacgagcg tgccgtcgtc gcgcgacaga aagtgcacgt
     2101 aaccctgata atcgcccagc acggcggcat gacccagcac gaacgggacg ctgagctcgc
     2161 ggttcttcag cttgtcgttc ttccacagcg gggcgccgtt gctgacgtcg aacgccgaca
     2221 ctaccgacca gtcgtcggcg gccaccacgg cgcggtcatc ttgcgcgagg ccgctcgtgc
     2281 tcgagaacgc cttttcccac accgcccggc ccgaattcgc gtcgaagcag ccaatctggc
     2341 cctggaacgt caccgcgcag gtttccgaac cgaccagcgt gggcggaccg gtcacgtcat
     2401 tgatgcgctc cacttccgtc acgcccttcg gatacgacac cggcgtttgc cagtagttgt
     2461 caccggtctg caggttgatc gcggcgaacg agccacccgg gaagcccgcc agcacagccg
     2521 cgtcgcccgc gaacgtcatg cccgacgaga cgcgcagatt gagcggcacc gcgcggttgc
     2581 gatagttcca cttctgctcg ccggtttgcg cattgaacgc gacgatctga ccgtcgaccg
     2641 tgcgcaccac cacgaggccg ttgccgacca gcggcggcga gatgatttca cccggcgcct
     2701 tggcggtcca caattgcttg ccgtccgcac caagcacgta gacgtcgccc ttcaggccgc
     2761 cgaccgccgt gagcgtgccg tcactgccga cgcctgccga cagatcgtca tgcagcttga
     2821 cgcgccagat gtcctgaccc gtttgcgcgt cgatcttcgc aaccgagcca ttcgcgccgg
     2881 ccgcgtacac cgcgttgccg accgcgactg gcgagaacag gtaacgaccc gccttgccca
     2941 cgctggcctt ccacgcctgc tgcacgtcga gcacgggttt gaactcggtg agcagcgtcg
     3001 gcacgcggcg ctcgtctttc gtggatgagc aagccgccat ggtgagcacg gtcatcgcac
     3061 aggcaacggg cacagcgtaa cgtttcagca gattcatcgg tggacgaagc attcaggaaa
     3121 ttaattaaag gagaccggtt ggtcgttaca tgcggcgcgc tgcatgcggc ggtcagccgc
     3181 ccagcgcgtc cagcttgaac tgaatcaact ggcgagccga gctatcgttt ttcgacagcg
     3241 tgtcgagcgc gagcttgtag gccgcgcgcg cgtcgtcgcg cttgccttgt gcggcgagca
     3301 gatcaccgcg gccgttcgcc acaatgccct tgaacgcatc ggactgcggt tcggcgagca
     3361 gtgcgaggcc ctggtcgtaa gccttgtcgt cgagcagcag ggaagcgagg cgcagcttgg
     3421 cgatctgttt gaactcgtcg tctttggcgt gatcgatggt ccattgcaac tgggccttcg
     3481 cggcggcttc atcacccgcg gcgtacagcg ccttggcagc gcccagcgca gtcatctgcg
     3541 catacgcggt gcggctgaac ttgtcttcca tgtcggtggc gacgcgcgtg atcttcgcct
     3601 tgtcgcccga tgccacggct tgctgcacct ggtcatacag caccgcggcc tccgttgact
     3661 gacgccgttg ccagaaattc cagccgttcc agccggctgc ggcaaccaat gccaccagca
     3721 cgatccacgt ggttgcattg ccccactgcg tccaccatgc cttcagactt tcaatcgatt
     3781 cttgttcgtc gtggtaactc atcgcccggc gatttcctct ttctcgatac gtgtgcatgt
     3841 cgagccacgg ggctcgacga catcgcgatc agtcgtcgcc gtcatcggcg gttgcaacca
     3901 tcgcattgat tagaaattcg gtcaagtctt cggcgggcac gttgtgttgc tcgttcttac
     3961 cgccgttagc gcccgtatcg cgcagcggct tcacgccgac cgtgccgttg gcgatctcgt
     4021 cttcgccgag gatcaccgcg aacgccgcgc cgctcgcatc agcgcgcttc atttgcgact
     4081 tgaagctcgc cgtttgaccg tcggcgctgc aatgcaggat cacgtcgagg cccgtatcgc
     4141 gcaaacgctc ggcgatgata aaggcctgtt cgcgtgccgc gtcgccctga tggaccacgt
     4201 acacgtcgca accttcgtct tccggcacga gttgctcttc tttcagcaac tcgagaatac
     4261 gttcgacacc catcgcccag ccgcacgcac ccgtcggctt gccgccgagc tgttcgatca
     4321 gcggatcgta acggccgccg gccgcgaccg tgccttgcgc gccaagcttg tcggtcaccc
     4381 attcgaacac ggtcagattg tagtaatcga gaccgcgcac cagacgcgga ttgatcgtga
     4441 acggaatatt gttcgccttc agaatgcgct gcagaccttc gaagtgcgca cgcgattctt
     4501 cgccgagaaa atcgatcagc ttgggcgcgt tctgcgcgac atcctgcata gccggattct
     4561 tcgtgtcgag cacgcgcagc gggttcgcgt acagacgacg ctttgcctct tcgtcgagca
     4621 cgtccatgtg cttttcgagg taagcgatca actcgacacg atgtgccgcg cgctcttcca
     4681 cgagacccag cgaattgagt tcgagcttga tgcccatcaa gccgaggtcg tcccacagac
     4741 gctggcacat catgatgatt tcagcgtcgg tgtccggtcc ggcgaagccc aatgcttcca
     4801 cgccgacctg atggaactgc cgataacggc cgcgctgcgg acgctcgtga cggaacatcg
     4861 gcccgatgta ccacagacgc ttcggaccgt cgtacagcat gttgtgctcg atcgtggcgc
     4921 gcacgactgc cgccgtgttt tccgggcgca tcgtcagatt ttcgccgttc aacgcgtcag
     4981 tgaagctgta catctctttc tcgacgatgt cggtcacttc
//
LOCUS       DATASH010000360         3264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_249230,
            whole genome shotgun sequence.
ACCESSION   DATASH010000360 DATASH010000000
VERSION     DATASH010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249230"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..1312)
                     /locus_tag="VHV99_10150"
     CDS             complement(149..1312)
                     /locus_tag="VHV99_10150"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX3635834.1"
                     /translation="MHDANPADLKAPPAEAPAAVASTPLSVITGFLGSGKTTTLSHLL
                     ADPRMNKAAVIINEFGEMGLDHLLVARPREDVILLANGCLCCTVRGELVDTLSELFVA
                     RARGEIMDFDRIVVETTGLADPVPVLRTAHFDADLGSLLHLENVITTVDAVHGLEQIA
                     SHPECVKQICTADTVLITKTDLADRAQIERLRAVIDELNPGVEQIEVLNGKVLPERLF
                     DTLNPQGLQTARLPKWLAASGEKHGHAHAPDAHDPHEHHHGHAAHHREGITSFPVYID
                     RPVTATGLQIWTQMLSDFNGADLLRIKGIVNIEGRPYVLQAVQHLIHPLAELPEWPSD
                     DRRSQLIFITRNISRADIEDTLHALQFDLPKKAAAGFDAEGFKRFAELAGTFR"
     gene            1640..2422
                     /locus_tag="VHV99_10155"
     CDS             1640..2422
                     /locus_tag="VHV99_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635835.1"
                     /translation="MRKIAVSLFLGVLLFVVAVAPCHAQATRTWVSGVGSDANPCSRT
                     APCITLAGAISKTAAGGEISVLDPGGIGAVTITKSISIVSEGSEGSILGCSTSGITIN
                     AAATDVVSIHGVFFEGCGNGLNGIKVISAKEVHIRKCLIRNFKSASGTGILVAPTSAG
                     VNVNVFVSECTIASNARGIATAPTAGTATTFLDRVVVEDSGLAGILSAGANATTYLSN
                     SVVGKNLLGLKVTGGGSIISFGNNTIGNNTTDGAPTSTLTLK"
     gene            2437..3153
                     /locus_tag="VHV99_10160"
     CDS             2437..3153
                     /locus_tag="VHV99_10160"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HEX3635836.1"
                     /translation="MESTRSGQNELPLKIITGFKPDNPIRSLYTESLQRAQDGKYDNL
                     LKQMRYYVLHQLATHAVEKFPALNFAECGCWWGHSTHILSRILEAHSGFSGRLHVFDS
                     FEGLSEFKPQDYSEYRSTPAMQERARRNFRSDLERVASGLSGFPFVTLHPGWIPAKFF
                     EVEDQEFSLVTVDVDLYEPIRDAMQFFYPRLRQGGVMYFDDYGYETFPGAKLAVDEFR
                     KTVTPQFFLEMPMGSAFLIK"
BASE COUNT          612 a          933 c         1056 g          663 t
ORIGIN      
        1 cgacgccggc gagcgtggcg tccggatgca tggacgatgc catggcgcgc cgtccccaca
       61 cttcggtgat cgggcgcgcc agagtctgtt gcgcgaaagt ggccgatgtg gcggcgctgc
      121 cggatgcggg agccatcgtc acaccggact agcgaaacgt gccggcgagt tccgcgaagc
      181 gcttgaagcc ttcggcgtcg aagccggcgg cggccttctt cggcagatcg aactgcagcg
      241 catgcagcgt atcttcgata tcggcgcgcg agatattgcg ggtgatgaag atgagctgcg
      301 aacgccggtc gtccgacggc cattcgggca attcggcgag gggatggatc aggtgctgca
      361 cggcctgcaa tacgtaaggc cgcccctcga tgttgacgat gcccttgatg cgcagcagat
      421 cggcgccgtt gaagtcgctc agcatctgcg tccagatctg caggccggtg gccgtcaccg
      481 gccggtcgat atagaccgga aacgacgtta tgccctcgcg gtggtgcgcc gcatgaccgt
      541 ggtgatgttc gtgcgggtca tgcgcgtccg gcgcatgcgc gtggccgtgc ttttcgccgg
      601 acgccgcgag ccacttcggc aatcgggccg tctgcagccc ctgcgggttc aaggtgtcga
      661 acaggcgctc cggcaacacc ttgccgttca gcacctcgat ctgctcgacc cccgggttca
      721 actcgtcgat gactgcgcgc agccgctcga tctgcgcgcg gtccgccaga tcggtcttgg
      781 tgatgagcac ggtatcggcg gtgcagatct gcttcacgca ttccggatgc gaggcgatct
      841 gctccaggcc gtgcacggca tcgacggtgg tgatcacgtt ttccaggtgc agcaggctgc
      901 cgaggtcggc gtcgaaatgg gcggtgcgca gaacgggcac cggatcggcc aagccggtcg
      961 tctccaccac gatgcgatcg aaatccatga tctcgccgcg cgctcgcgcg acgaagagct
     1021 cgctgagcgt gtcgaccagc tcgccgcgga cggtgcagca gaggcagccg ttcgccagca
     1081 ggatgacgtc ctcgcgcggc cgcgccacca gcagatggtc gagccccatc tcgccgaact
     1141 cgttgatgat gacggcggct ttgttcatgc gcggatcggc gagcaggtgc gagagcgtgg
     1201 tcgtcttgcc gctgccgaga aagccggtga tgacgctgag cggcgtcgac gccacggcag
     1261 cgggggcctc cgcgggcggg gctttcaggt cggcggggtt ggcatcgtgc atggtgcagg
     1321 ctatccggtc gtgttttact agtgaaacat catttttaat atgatacacg gtggccatgg
     1381 ggtgggaaag gcctcgtcaa cgatctgcat gccgccggcc ggtgcgcgga caaccgttgg
     1441 ttctcaaatt gggctatggc gccggttctc ctccgggatt gccagttgcg gtggccaata
     1501 tgcgcggccg ccgcggcgcc gaaacgccga atccgaactt ccggcaggcg tggaatgggt
     1561 ctattattga tgatgaattg gcgctgaaaa aatgttgctc gaacgtatag atcaatcgtg
     1621 aattaaacgg gggccgacga tgcgaaaaat cgcagtttcg cttttcctgg gcgttctctt
     1681 gtttgtcgtt gcggttgcgc catgtcatgc gcaagcgacg cgaacctggg tttccggcgt
     1741 cggcagcgac gccaatccgt gctcacgaac agcgccgtgc atcaccttgg ccggggcgat
     1801 ttcaaaaact gcggctggcg gtgagattag cgtgctcgat ccgggcggca tcggcgctgt
     1861 gacgatcacc aaatcgatca gcattgtgtc ggaaggttcc gagggcagca ttcttggttg
     1921 cagtacgagt ggtatcacca tcaatgcggc ggccacggat gtcgttagta tccatggtgt
     1981 cttctttgaa ggctgcggaa atggtttgaa cggcatcaaa gtcatctcgg caaaggaagt
     2041 tcatattcgc aaatgtctta tccgtaattt caaatcggcg tcgggcacgg ggattctcgt
     2101 tgcgccgacg agcgcgggcg tgaacgtcaa cgtctttgtg tccgaatgca cgattgccag
     2161 caacgcacgg ggtattgcca cggccccgac ggctggaacg gcgacgacct ttctcgatcg
     2221 cgtcgtggta gaagacagcg ggctcgcagg cattctgtcc gccggcgcaa atgccacgac
     2281 ctatttaagt aattcagtcg taggcaagaa cttgctaggc ctgaaagtga cgggcggagg
     2341 aagcatcatt tcattcggca acaataccat cggaaataat accactgacg gggctccgac
     2401 cagtacgctg acactgaaat agcgaaggca ggcagagtgg aatccacgag atcaggccag
     2461 aatgaacttc cgctgaagat catcacgggc ttcaagccgg acaatccgat caggagcctc
     2521 tacaccgaga gtcttcagcg cgcccaggac ggcaagtacg ataatctgct caagcagatg
     2581 cgctactacg tgctgcacca gctcgcgacc catgcggtgg agaaattccc cgcgctgaat
     2641 tttgccgaat gcggatgctg gtggggtcat tcgacgcata ttctgtcgcg gatactggaa
     2701 gcgcattctg gtttctccgg ccgccttcac gtcttcgatt cattcgaggg cttgtcggag
     2761 ttcaagccgc aggattattc cgaatatcgt tccacgccgg cgatgcagga gagggcgcgg
     2821 cggaacttcc gttccgacct ggagcgcgtc gcgtcgggtc tttccggctt tccgtttgtg
     2881 acgctgcatc cgggatggat accggccaag tttttcgagg ttgaggatca ggaattctcg
     2941 ctggtcacgg tcgacgtcga cctgtacgag ccgatccgcg acgcgatgca gttcttctac
     3001 ccgcggcttc gtcagggcgg ggtgatgtat ttcgacgatt acggctacga gacgttcccc
     3061 ggcgcgaagc ttgccgtcga tgaattccgc aagacggtga cgccgcaatt cttcctggaa
     3121 atgccgatgg gatcggcctt cctgatcaaa tagcaggttc gcgcgtcagc gccgcgcgtt
     3181 cggcgagcca gaggtcgcca tagtcgacat tctgccaggc gtcgaaccat gggccgccgc
     3241 gcgtgaaatg gaccaccttc ggcg
//
LOCUS       DATASH010000361         2754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4404851,
            whole genome shotgun sequence.
ACCESSION   DATASH010000361 DATASH010000000
VERSION     DATASH010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4404851"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="VHV99_10165"
     CDS             <1..823
                     /locus_tag="VHV99_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3635837.1"
                     /translation="AGVLGGPLSGWIMQAFNGVHGLANWQWLFVLEGIPSIVLGVLTL
                     KYLPNGIRSAKWLTEDEKTLLEQRVAEDGTGKLHASLSSVLSNGRVWFMAGIYFCCMM
                     GLYGVSFYLPTLIKTAGVKDALNVGMLTAIPYGCAIVAMLLIARSADRLRERRWHFAA
                     AGLMASLGLLAATVFSSNVVLAMIALSVGTAGVLATMPVFWAWPGAMLGGTAAAAGIG
                     LINSVGNLAGFVSPTVVGWMKDVTNSTNAGMYVIAAALFAGACLALFQPREKVGK"
     gene            complement(1511..2191)
                     /locus_tag="VHV99_10170"
     CDS             complement(1511..2191)
                     /locus_tag="VHV99_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014971963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX3635838.1"
                     /translation="MATLYDTLGVHAHATDEEIKRAYRKAAMKWHPDRNSGAEEVARA
                     TFQEIKDAYAILSDAAQRKVYDAVYSEQMRGWVAQRTRQQKAQAEREAAARAADEAAY
                     AEMVSLAMRFADDGHNRDVLFGVLLGRRCEARRAAQIADSVSALQASRREAEKAEAAA
                     EAAAKTAHVPDVSASTEAATVNDGAANHGTPGNHGNPGKREENASDAHPAGVLSGLWF
                     QFLNGLRF"
     gene            2660..>2754
                     /locus_tag="VHV99_10175"
     CDS             2660..>2754
                     /locus_tag="VHV99_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN reductase"
                     /protein_id="HEX3635839.1"
                     /translation="MTSFDHLRRPLVIGIGGTTRAASSTERALGFA"
BASE COUNT          487 a          880 c          832 g          555 t
ORIGIN      
        1 cgccggagtg ctcggtggtc cgttgtcagg ctggatcatg caggcattca atggcgtgca
       61 tggtctcgcg aactggcagt ggcttttcgt gctggaaggc attccttcga tcgtgctcgg
      121 tgtcctcacg ctgaagtacc tgcctaacgg aatccgttcg gcaaaatggc ttaccgaaga
      181 tgaaaagaca ttgctcgaac aacgcgtcgc ggaagatggc accggcaagc tgcacgcatc
      241 gttgtcgagc gtgttgagca acggccgggt gtggttcatg gccgggattt acttctgctg
      301 catgatgggt ctctacggcg tgagctttta tctgccaacg ctcatcaaga ccgcgggcgt
      361 taaggatgcc ttgaacgtcg gcatgctcac cgcgatccct tatgggtgtg cgatcgtcgc
      421 catgctattg atcgcccgca gcgccgaccg cttgcgcgag cgacgctggc atttcgctgc
      481 cgcgggtctg atggcgagcc tggggctgct ggccgctact gttttcagtt cgaacgtcgt
      541 ccttgcgatg atcgcgcttt cagtcggcac ggcgggcgtg ctcgccacga tgcccgtttt
      601 ctgggcgtgg cctggcgcga tgctcggtgg caccgcggcc gccgccggca tcggtctgat
      661 caactcggtt ggcaacctgg cggggttcgt cagcccgacc gtcgtcgggt ggatgaagga
      721 cgtcacgaac agcaccaatg cgggcatgta tgtgattgca gccgcgctct tcgccggcgc
      781 atgtctcgcc ttattccaac cgcgcgagaa ggtcggcaag taagcgcctg aaggcggcct
      841 cagtgccgcc tggatgtgtc gcctggatgc cgcgcgccgg agtgccgttt gccgtgccgc
      901 ctgagtggcc gcctgtgagt ggcgcctctg agtggccgcc tgtgtgcccg cctgcgtgcc
      961 gcgtcaatac ctctgtaatg ccgccgcgtt ctcgcggggc ggcatctctt cagacagaag
     1021 ccccactttc gcgccggtcg gacacgaccc gattcgcagc gtcacttgcg ccaccagacg
     1081 ccaccggtga agtttcgaac cgcatcgccg cgggctgcgc gctgatcgcc ttgaccccgt
     1141 tcacgatgcc aggcgccgcg acgtagggga taaacgccgc cactgcaacc acaaaaataa
     1201 aggtaatcat ggccgtcgtg cctctcggaa tctgcaacat ggaggtcaag cgaagcgggt
     1261 ctttcgcgaa ggaagtcgag ccagcgacag gcagatgagc agcgggcacg cgtgccacct
     1321 tgtacccggc ataccgacga gtccgccacc cgcatgcgtg gcaatgagga accgccgttc
     1381 ttcatccggc aatagaacga tacatgtcac ggacatgtct ttgattctag aagtggatat
     1441 tcggacgcta aaggccgcgc tctatatcaa ttcggtctac gtctcgggtc taacggccaa
     1501 aaccatctca tcaaaaccgc aagccattca ggaactgaaa ccacaggcca ctcaatacgc
     1561 cggcaggatg agcatcactg gcattttctt cgcgcttgcc tgggttgccg tggttacctg
     1621 gtgtgccatg gttggcggcg ccgtcgttga ccgtagctgc ctccgtgctg gctgaaacgt
     1681 caggcacgtg cgccgttttc gccgccgcct cggcggcagc ctccgccttc tcggcctcgc
     1741 gccgcgacgc ctgcaatgcc gacacactgt cggcgatctg cgcggcccgc cgcgcctcgc
     1801 atcgacgtcc cagcagcacg ccgaataaca catcgcgatt gtggccgtca tcggcgaaac
     1861 gcatggcgag cgacaccatc tcggcatagg ccgcttcgtc cgcggcacgc gccgccgcct
     1921 cgcgttcggc ttgcgctttt tgctgacgcg tccgctgcgc tacccagccg cgcatctgct
     1981 cgctatagac cgcgtcgtac actttgcgct gcgcggcgtc ggagaggatc gcgtacgcgt
     2041 ccttgatttc ctggaaggtg gcgcgcgcca cttcttcggc gccgctgtta cggtccggat
     2101 gccatttcat cgcggccttg cgataggccc gcttgatttc ttcgtccgtg gcgtgcgcgt
     2161 gcacaccgag cgtgtcatat agggttgcca tcgcttgctc gacctcgccg ggaatcactg
     2221 gcggaccatc gtagcatgca aaaaacggct ttatcgtgaa gcagcaagcg ctgacgcgac
     2281 tgaatgaccg gacaaagacc ccaaaaaaaa gcctcaccac tggcgtagcg aggccgagtc
     2341 ccatgtcaag gagatgtcat ggaggagaca gctcaatcat agccgcgcgt catcgcgccc
     2401 ccaaacaatg gttccccata tgcttatccc gcgtggcttc aactgcattg cacagggccg
     2461 gagatctgcg tcgaaactgc catcagacag cccatcgggg cgccgataac cccgcgcggt
     2521 tatatcgtta gaacaaaatg tcgcttcgcc ggggtcgcgg cgatcattaa gatgacactt
     2581 caaagtccgg ttcgccccgg tacgtctgcg cgcagccccg cgccagcgcc ccgtgagccg
     2641 ctaccgaagg agcatttcgt tgaccagttt cgaccatctc cgccggccgc tcgttatcgg
     2701 cattggcggc acgacacgcg cggcgtcgtc gaccgagcgc gcgctcggct ttgc
//
LOCUS       DATASH010000362         2199 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_446867,
            whole genome shotgun sequence.
ACCESSION   DATASH010000362 DATASH010000000
VERSION     DATASH010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446867"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(488..1132)
                     /locus_tag="VHV99_10180"
     CDS             complement(488..1132)
                     /locus_tag="VHV99_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009908400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3635840.1"
                     /translation="MNVHTKKYLIDGPVGKIEVALDLPDEMRESGAAPRGIALVAHPH
                     PLFGGTMDNKVAQTLARTLVQLNYVTYRSNFRGVGETQGEHDAGIGERDDLRAVLDHM
                     RAQPGQGNLPLVLAGFSFGTFVLSHVAAKLREEGRAIERIVFVGTAASRWEVAPVPEN
                     TLVIHGETDDTVPIQSVYDWARPQELPVVVIPGAEHFLHRKLHVLKRIIVDAWR"
     gene            complement(1141..1464)
                     /locus_tag="VHV99_10185"
     CDS             complement(1141..1464)
                     /locus_tag="VHV99_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEX3635841.1"
                     /translation="MDAFYKYHVFFCLNQRDPGAERPSCANCNAQEMQEHAKKRVKKL
                     GLAGPGQVRINKAGCLDRCELGPALVVYPEGVWYTYVDESDIDEIVDSHLVNGKIVER
                     LKIDQ"
     gene            complement(1617..>2199)
                     /locus_tag="VHV99_10190"
     CDS             complement(1617..>2199)
                     /locus_tag="VHV99_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="teicoplanin resistance protein VanZ"
                     /protein_id="HEX3635842.1"
                     /translation="FDSTTQDALLAWTPPAWHVGAWPALVGAWLPDDIWEALITTLNL
                     FAALALASLPVRRHAPLVRLLVVFIVTTLCVKAGATFLQSQSGLAFDWATPGALAGLV
                     CGTVAALLALRLRPRSRAALAACALTVALVFVNLLPVNPYFDAVLADWRQGRYLHFNG
                     LAHWIAWIWPYAALVWLAYLFEQAWLKRRARRA"
BASE COUNT          418 a          694 c          724 g          363 t
ORIGIN      
        1 ccgtgctggc cacgagcgtt gccggcagca cgatgccgag ggtcacggca cggctaagcg
       61 tatggggaac gaaagaagaa actgacggga atgacgtgcg gccggaggtg gagaaacgca
      121 tagggtcgat tcgtgcggaa agatacatcg tgcgcagggc gcatagtttg gaggagccgg
      181 tcggcaagcg tcgggccgta aaaacggcgc ggcgcccatt ggacttccgg ccacgcgatc
      241 ggttcgtgca gcacgcctcg catccggcac cgctgcgcgc gagaaagaag ctacgcgtcg
      301 cgcgaactgc cgggtgggcg atgctgcgcc caaaaaagag cgcccattat acgcgcgcta
      361 tcctggtatc ctgacccgca gggcagcgat taatttgcgt ttgcacgtac ctgtaccccg
      421 caaaacacgc gcggcgcgcc ccgcgtgttt tgcggcgtgt gccccgcgta tgccccgcgt
      481 gtgcccatca gcgccacgcg tcgacgatga tccgcttcag gacgtgcagc ttgcgatgca
      541 gaaaatgctc tgcccccgga atcacgacga ccggcagttc ctgcggccgc gcccaatcgt
      601 aaaccgactg aatgggaacg gtgtcatcgg tttcgccgtg aatcacaagc gtgttttccg
      661 gcacgggtgc gacttcccag cggcttgccg ccgtgccgac gaacacgatc cgctcgatcg
      721 cccggccttc ttcgcgcagc ttcgccgcca cgtgcgacaa cacaaaggtg ccgaacgaaa
      781 aacccgccag caccaggggc agattgcctt gcccgggttg cgcgcgcatg tgatcgagca
      841 cggcacgcag atcgtcgcgc tcgccgatgc cggcgtcatg ctcgccttgc gtttcgccga
      901 caccccgaaa attcgagcgg taggtcacgt agttcaactg cacgagcgtg cgcgcgagcg
      961 tctgcgcgac cttgttgtcc atcgtgccgc cgaacagcgg gtgcggatgc gcgacgagcg
     1021 caatgccgcg cggcgcggcg ccgctctcgc gcatttcgtc gggcaaatcc agcgcgactt
     1081 cgatcttgcc gaccgggccg tcgatcagat acttctttgt atgtacgttc atggcgaggc
     1141 ttattgatcg atttttagac gctcgacgat tttgccgttc acaaggtgcg aatcgacaat
     1201 ctcgtcgatg tcgctttcgt cgacgtacgt gtaccagacg ccttcgggat aaacgaccag
     1261 cgcggggccg agttcgcagc gatccaggca gccggccttg ttgatacgca cctgcccggg
     1321 accggcgagg cccagtttct tcacgcgctt tttcgcgtgc tcctgcatct cctgcgcatt
     1381 gcaattcgcg caactcgggc gttccgcgcc ggggtcgcgc tgattgaggc agaagaagac
     1441 gtggtacttg tagaaggcgt ccatgatgtc cgggggcgag actgtgagag taggaaatgg
     1501 cgctgggagc gttgcggcgc gcctgttgtg cggacgatta tagcgagcgg gccaggcagt
     1561 cgctgaacgg cggacgggcg gtggtatgcg gtcgggctgg gcgtgcagct cctgcgtcat
     1621 gcgcgccttg cgcggcgctt gagccaagcc tgttcgaaga gataggcgag ccacaccagc
     1681 gccgcatacg gccagatcca cgcaatccag tgcgcgagac cgttgaagtg cagataccgg
     1741 ccctggcgcc agtcggccag cacggcgtcg aaatacggat tgaccggcag cagattgacg
     1801 aacaccagcg cgaccgtgag cgcgcaggcc gcaagcgccg cacgcgacct gggccgcagc
     1861 cgcagcgcca gcagtgccgc gacagtgccg cacacgagcc ccgccagcgc gccgggcgtc
     1921 gcccaatcga acgcaagacc ggattgcgat tgcagaaacg ttgcgcccgc cttgacgcac
     1981 aaggtcgtga cgatgaacac gaccaacaga cgcacgagcg gcgcatggcg gcgcaccggc
     2041 agcgaagcca gcgcgagtgc ggcgaacagg ttgagcgtgg taatgagggc ttcccagatg
     2101 tcatccggca gccaggcgcc aaccagcgcc ggccacgcgc cgacgtgcca ggctggcggc
     2161 gtccatgcga gcaacgcgtc ttgcgtcgtc gaatcgaaa
//
LOCUS       DATASH010000363         2932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2427216,
            whole genome shotgun sequence.
ACCESSION   DATASH010000363 DATASH010000000
VERSION     DATASH010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2427216"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2136)
                     /locus_tag="VHV99_10195"
     CDS             complement(<1..2136)
                     /locus_tag="VHV99_10195"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX3635843.1"
                     /translation="MNRSTPMTAYIGTATSRVDGRAKVTGAAKYAAEFNTPGLAHASV
                     VASAIAKGRIVRIDAREALAVDGVIDVLTHQNRPPMAGADSAYKDEVAPEGSPFRPLY
                     DDRIWFSGQPVALVVAEEPEVARFAASLVRVEYDKVAHVTDLYRQRDAAFALKAPADP
                     AENPFAPPKPRGTAEPALAAAAVRHRGEYYVPIEHHNPMELFASTVIFEADGKLAVYD
                     KTQGVQNVQRYLCSVLEMESHEVRVISPFVGGAFGSALRPQFQVVLAALAARALQRSV
                     RLVLTRQQMYGLGYRPAMIQQIELGAKSGGTLDVITHDAITVTSQYEDFYRQETGWSG
                     LLYEAPNAKYAHKLARLDLATPCDMRAPSAATAVFALECAMDELAVALKLDPLELRLR
                     CYSERDQHSDRPYSSKSLRDCYRQGAETFSWDKRNTEPRSMRDGTELVGWGMATGVWE
                     ALQVPIAVRIVLTANGHAEVSCATSDIGTGTYTIMAQVAADMLGLPLDNVTIKLGDSS
                     LPQSPVEGGSFIAASVSNGIVTTADAIRRELLRLAKQMPNSPLADATPDEVALADGRL
                     VNKRDASRAVSIADAMRHGAVDRIEQEKSTTFEDDGSHAQNTHSAVFAEVKVDEELGV
                     IRVTRVVSAVAAGRILNPKTARSQIVGGVVWGIGMALHEGTLVDHNFGRIMNANIAEY
                     HVPVNADVHDISVIFVDEPDDIVNPLGIKG"
     gene            complement(2133..>2932)
                     /locus_tag="VHV99_10200"
     CDS             complement(2133..>2932)
                     /locus_tag="VHV99_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014750202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX3635844.1"
                     /translation="PNSDLAYQPLIAQRYPMLASAILAGASAQLRNMASTGGNLLQRT
                     RCCYFYDTATPCNKREPGSGCSAAMGLNRMHAILGTSEDCIATHPSDMCVALAALEAK
                     VHVAGPAGARTIAFADFHRLPGNTPQRDTNLPPDEIVTAVELPPRGFAANYTYLKIRD
                     RLSYAFALVSVAAALELEGNTIKEARLALGGVAHKPWRDSSAEAALRGQTANQSTFSR
                     AADVLLRDAKGFQHNAFKIDLARRAIVRALTQAAAGTPQSQSNKKIR"
BASE COUNT          489 a          932 c          961 g          550 t
ORIGIN      
        1 tcccttgatg ccgagtgggt tgacgatgtc gtctggctcg tcgacgaaga taacactgat
       61 gtcgtgcacg tcggcgttca ccgggacatg atactcggca atgttcgcat tcatgatgcg
      121 accgaaattg tggtcgacga gcgttccctc gtgcaacgcc atgccgatgc cccacaccac
      181 gccgccgacg atctggctgc gcgcggtctt ggggttgagg atgcggccgg ccgcgactgc
      241 gctgacgacg cgggtgacgc ggatgacgcc gagctcctcg tccaccttca cttcggcgaa
      301 gaccgccgag tgcgtgtttt gcgcatgcga gccgtcatct tcaaaggtgg tggatttctc
      361 ctgctcgatc cggtcgacag cgccatgccg catcgcatct gcaatggaca ccgcgcgcga
      421 cgcatcgcgc ttgttgacga gcctgccgtc tgcaagcgcg acctcgtcag gcgttgcatc
      481 cgcgagcggc gaattcggca tctgctttgc taggcgcaac agttccctgc ggatcgcgtc
      541 ggctgtcgtc acgatgccat tcgacaccga ggccgcaata aatgatccgc cttcgaccgg
      601 cgattgcggc aagctcgagt caccgagctt gatcgtgaca ttgtcgagag gcagccccag
      661 catgtcggcc gcgacctgcg ccatgatcgt gtaggtgccg gtgccgatgt cggaggtcgc
      721 acacgaaacc tccgcatggc cgtttgcagt aagcacgatg cgcaccgcaa tcggcacctg
      781 cagcgcttcc cacacgccgg tcgccatgcc ccagcctacc agctcggtgc cgtcgcgcat
      841 cgagcgtggc tcggtgttgc gcttgtccca gctgaaagtt tccgcgccct ggcggtagca
      901 atcgcgcaag cttttgctgc tgtacggccg gtcgctgtgc tggtcgcgtt ccgaatagca
      961 ccgcaggcgc agctcgagcg gatcgagttt gagcgcgacc gcgagttcgt ccattgcgca
     1021 ttcgagtgcg aagacggcag tcgcggcact gggggcgcgc atgtcgcacg gggtcgcaag
     1081 atcgagccgc gcgagtttgt gcgcgtattt cgcgttgggg gcttcgtaga ggagcccgga
     1141 ccagccggtc tcttgccggt agaaatcctc gtattgcgat gtcaccgtga tcgcgtcgtg
     1201 agtgatcacg tcgagagttc caccggattt tgcgcccagc tcgatctgct ggatcatcgc
     1261 ggggcggtag ccgagcccat acatctgctg ccgggtcagc acgagacgca ccgagcgctg
     1321 cagcgcacgc gccgccagcg cggccagcac cacctgaaac tgcggacgca gcgcggaacc
     1381 gaacgcgccg cccacaaatg gcgatatgac gcgcacctcg tgcgattcca tttcgagcac
     1441 gctgcaaaga tagcgctgaa cgttctgcac gccctgggtc ttgtcgtaga ccgccagctt
     1501 cccgtctgcc tcaaagatca ccgtcgaagc aaagagctcc atcgggttgt gatgctcgat
     1561 cgggacataa tattcgccgc ggtggcgcac tgccgctgcg gccaacgccg gctcggccgt
     1621 gccacgaggc tttggcggag cgaatgggtt ctcggccggg tctgcggggg ctttcaacgc
     1681 gaaggccgca tcacgctggc gatacaaatc cgtgacgtgc gctactttgt cgtattccac
     1741 ccggaccagc gacgccgcga agcgggcgac ttccggctct tccgcgacca ccagtgcaac
     1801 cggctggccg ctgaaccaaa tcctgtcgtc gtagagtggc cggaacggcg aaccctccgg
     1861 agccacctcg tccttgtacg cgctgtcggc gcccgccata ggcggccggt tctgatgcgt
     1921 tagcacatcg attacgccgt cgacagcaag cgcctcgcgc gcgtcaatgc gcacgatgcg
     1981 gcccttggcg atcgcagacg caacgacgct cgcatgcgcg aggccgggcg tgttgaactc
     2041 ggcggcgtat ttcgccgcgc cggtgacttt ggcgcggcca tcgacgcggg atgtggcagt
     2101 gccgatgtaa gccgtcatgg gcgtgctcct gttcatcgga ttttcttatt ggactgcgac
     2161 tggggggtgc cggccgccgc ctgcgtcaac gcacggacga tggcgcggcg agcgagatcg
     2221 atcttgaagg cgttgtgctg gaagcctttg gcgtcgcgca gcaagacgtc cgcagcgcgg
     2281 ctgaaggtcg actggttggc ggtctgcccg cgcagcgccg cttcggctga cgagtcgcgc
     2341 caaggcttgt gcgcaacgcc accgagcgcc agacgggcct ctttaattgt gtttccttcg
     2401 agttccagcg ccgccgcgac agacacgagc gcgaacgcat aggagaggcg atcacggatc
     2461 ttcaggtacg tgtagtttgc agcaaagccc cttggcggga gctcgaccgc cgtgacgatc
     2521 tcgtctggtg gcagattcgt gtcgcgctgc ggcgtgttcc ccggcagccg atggaaatcc
     2581 gcaaacgcaa tcgtgcgcgc ccctgccggt ccggccacat ggactttggc ctcgagcgcg
     2641 gccagcgcca cgcacatatc ggaggggtgc gtcgcaatgc agtcctcgct cgtgccgagg
     2701 atcgcgtgca tgcggttgag gcccatggca gcggagcagc cgctgccggg ctcgcgtttg
     2761 ttgcacgggg tcgcggtgtc gtagaagtaa cagcagcggg tgcgctgcag cagattgccg
     2821 ccggtcgacg ccatattgcg caattgcgcc gacgcgcccg ccaggatcgc gctcgcgagc
     2881 atcggatagc gctgcgcgat cagcggttga taggcgaggt cggaattcgg ca
//
LOCUS       DATASH010000364         2443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4008751,
            whole genome shotgun sequence.
ACCESSION   DATASH010000364 DATASH010000000
VERSION     DATASH010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4008751"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            259..1005
                     /gene="aqpZ"
                     /locus_tag="VHV99_10205"
     CDS             259..1005
                     /gene="aqpZ"
                     /locus_tag="VHV99_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HEX3635845.1"
                     /translation="MVALGKRLLVEGVGTAWVVFIGCGSIVLNTGAAQQGYGVLEVSL
                     AFGLALVTASYAFGAISGAHFNPAVTIGFTVAQRFPVRDLLPYMAAQILGAIAAAALL
                     AYVASGRPSFELGASEFAANGFGDHSPADYQLHSALVVELALSFAFVMASLMMTRQRR
                     LRPIAPLVAGACLAIAYMVSIPVTNGSINPARSTGQALFVGDWALDQLWLFWAAPLLG
                     GVVAGALFSFLCGKSGPHATSFAHEQGEVA"
     gene            1026..>2443
                     /locus_tag="VHV99_10210"
     CDS             1026..>2443
                     /locus_tag="VHV99_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX3635846.1"
                     /translation="MMLSSKLRRSVVCSVMMKQVLRRVSQCLLAVFMFAVTQMQPAVA
                     QPFTGQSHPSVGGAFPATLTVGVLANGWMPFDGLQDGQLTGLSGDYLRALVGPNVVIE
                     SKAYPDMPQLLAAACAGNVDLVMSLARTTERERCLSFTSPYFRASASVAVRRAGDVYA
                     SAAQLVGARLAVEKGFALERALRERFPRTQLETFASTHAALDAVARGDADAYLGFTPA
                     VQYALATEEFRGLRVAFEESGRIADLRFAVPRNRVALRDQLDRALASMNPADDTAIRV
                     RWLSGSFDAQAASAARRLVLTAEEQAWLRSQPPLQVGFDTGWPPFSYVDDSGHPAGIA
                     AEYLDYLSRALGVTFDRAHTADWPATLEAFQRGDLAILATSSGNDPRLKNALHTRAYE
                     SYPLVIVGRENEPAARALNDFASRRIVVSSHIAGSIPFAIDKIPTANITVAPSLDDAL
                     KMVENGQADVLVGNVAAVDILL"
BASE COUNT          382 a          754 c          783 g          524 t
ORIGIN      
        1 tagggttggc ctgacagtcg tgtgagagaa ccaggcctcg acagccgaca gttcttagga
       61 ctttaggagt cttctgattg gttgagggac attcccgttt catagttctg aattagctga
      121 ggctcgactt ccaacacccg catgaagaaa gcagccattt cgccatgcag tgcactgccg
      181 agcctgggaa attcaggcag atgtgtcgcg gctggaatgc ggcgccgtct gccatttgca
      241 caagtactgg gaggcgggat ggtagcgctg ggcaagcggt tattggtcga gggggtcggg
      301 acggcgtggg tggtgttcat cggctgcggc agcattgtat tgaacaccgg cgcggcgcag
      361 cagggctacg gcgtgctcga ggtatcactc gcgttcggtc tcgcgctcgt cacggcgagc
      421 tacgcattcg gcgccatctc cggcgcgcac ttcaatccgg ctgtgacgat cggctttacg
      481 gtcgcgcaac gtttcccagt tcgggatctg ctgccctaca tggccgccca gatcctcggg
      541 gccatcgcag ccgcggcgct gctcgcttac gtggcgagcg gccgcccgag cttcgagctg
      601 ggcgcgagcg aatttgcggc gaacggcttt ggcgaccatt ctccggccga ctatcaactt
      661 cattctgcgc tggtcgtcga acttgcgctg tcgttcgctt tcgtcatggc gagtctcatg
      721 atgacccgcc agcggaggtt gcggccgatc gcgccgctag tcgcgggcgc gtgtctggcg
      781 atcgcgtaca tggtgtcgat tccggtgacc aacggctcga tcaatcccgc tcgctccacc
      841 ggtcaggcat tgttcgtcgg cgactgggcg ctcgatcagc tctggttgtt ctgggcggcg
      901 cctttgctgg gtggcgtcgt cgccggtgcg ctgttttcat ttctgtgcgg caagtccggt
      961 ccgcatgcca cgtcgttcgc tcacgagcaa ggcgaggtcg cgtgagcgcc gcccggtgtc
     1021 atatcatgat gctctctagc aaactccggc gcagtgtcgt gtgcagcgtc atgatgaagc
     1081 aggtgctgcg acgcgtatcg cagtgcctgc ttgcagtgtt catgtttgcc gtcacgcaga
     1141 tgcagccggc agtcgcccag ccgttcaccg gtcaatcgca tccgtcggtc ggcggcgcct
     1201 ttcctgccac gttgactgtt ggcgttctgg ctaacggctg gatgcctttt gacgggcttc
     1261 aggacggtca gctaaccggc ttgagcggag actatctgcg tgcactggtt gggccgaacg
     1321 tcgtgatcga gtcgaaggcg tatcccgaca tgccgcaact gttggccgcc gcgtgcgccg
     1381 gcaatgtcga tctggtcatg agcctcgcgc gtacgacaga gcgggaacgc tgcttgagct
     1441 tcacctcgcc gtattttcgg gcgtcggcgt cggtggcggt tcgccgcgcc ggcgatgtct
     1501 acgccagcgc cgctcagctt gtcggcgcgc gcctcgcggt ggagaaaggg tttgcgctgg
     1561 aacgcgcgtt gcgcgagcgc tttcctcgca ctcagctcga aaccttcgca agtacgcatg
     1621 cggcgctcga tgcggttgcg cgaggcgatg ccgacgccta tctcggcttt actcctgccg
     1681 ttcaatacgc gctggccacc gaggaattcc gcggactgcg agtcgctttc gaggaaagcg
     1741 gcaggatcgc tgacttgcgt tttgccgtac cgcgcaacag ggtggcgttg cgcgaccagc
     1801 tcgatcgcgc gctcgcctcg atgaatccgg ccgacgacac ggcgatccgc gtgcgctggc
     1861 tatccggcag cttcgacgca caagctgcgt ccgccgcacg acgcctcgtg ctgactgcgg
     1921 aagagcaggc ctggcttcgt tcgcaaccgc cgttgcaggt gggtttcgat accggctggc
     1981 cgcctttcag ttatgtcgac gattccggcc atcctgcggg cattgccgcc gaatatctgg
     2041 actatttgag tcgtgcgctg ggcgtcacat tcgaccgggc ccacaccgca gactggcccg
     2101 cgactcttga agctttccag cggggcgatc tggcgattct ggccacctcc tccggtaacg
     2161 acccgcgtct gaagaacgcg ctgcacaccc gtgcatacga aagctatccg cttgtgatcg
     2221 tcggacgcga aaacgaacct gccgcgcgcg cgctcaacga tttcgcctcg cgtcgcattg
     2281 tggtctcgtc gcatatcgcc ggctcgattc cgtttgcgat cgacaagatc ccgactgcca
     2341 acatcactgt cgcgcccagt ctggacgacg cgctcaagat ggtcgagaac ggccaggcag
     2401 acgtactggt tggcaacgta gccgccgtcg atatcctgct gaa
//
LOCUS       DATASH010000365         4171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2427842,
            whole genome shotgun sequence.
ACCESSION   DATASH010000365 DATASH010000000
VERSION     DATASH010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4171
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2427842"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..137
                     /locus_tag="VHV99_10215"
     CDS             <1..137
                     /locus_tag="VHV99_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018417470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HEX3635847.1"
                     /translation="DPQPHPMKPGDHVLIEAHCRHRVAWTSGTEPSVWLAVYYPGVRR
                     "
     gene            218..604
                     /locus_tag="VHV99_10220"
     CDS             218..604
                     /locus_tag="VHV99_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011852989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEX3635848.1"
                     /translation="MSISIVQLGSPRTPDEGLRIGTVRRPPRGVPRTEFSTRDYYDVW
                     LPNLSPDAELMKEGKTAASDADWAVFAKKFRAEMNGSDTGKVLDLLAALSKTTHFAIG
                     CYCEDESRCHRGILRQLLSERGALIR"
     gene            838..1413
                     /locus_tag="VHV99_10225"
     CDS             838..1413
                     /locus_tag="VHV99_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018417471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEX3635849.1"
                     /translation="MGKGRVEAFSDGVIAIIITIMVLELKVPEGYDFAALRPVAPVFC
                     AYLLSFIYVGIYWNNHHHMFHAVQKVNGAVLWANLHLLFWLSLLPAVTHWVGENHLAS
                     WPTAMYGVVLFMSAVAYFILTRMLIREHGKDSTLAKAVGNDFKGKVSVIIYLSGIALA
                     FVQPWLSAALYTLAAAWWLVPDRRIEHVLEA"
     gene            1416..2225
                     /locus_tag="VHV99_10230"
     CDS             1416..2225
                     /locus_tag="VHV99_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635850.1"
                     /translation="MLLALKLALVPAFLAALTVAGRVWGPSVAGWLAGLPVIAGPIVL
                     LLALERGPAFAAQASAASIAAIAASEAFNFAYTWTCRRSAWPFSLLAGMLGWVGVAVL
                     LTHLPGGLPWAVAVGCVAVTVSQSGLPRVTGHVPAARLGLGDLALWMLAGALLTVAVT
                     TLSASMGATWSGLLSVFPLLGIVLAVSAQRAHGADFVALLMRGMVIGRGSFAAFFAVT
                     AMMLPHYGVWPSFACAALVSVIVQGVTKRMLAAKRPVQALTREFAEQQSAD"
     gene            2529..3017
                     /locus_tag="VHV99_10235"
     CDS             2529..3017
                     /locus_tag="VHV99_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX3635851.1"
                     /translation="MTSRTQRSSATRIGLISDTHNLVRPEALQYLSGCHAIIHAGDIC
                     NQAVLDALARIAPVTAVRGNNDIDEPVAALPTHVKLTVQQVTILVVHDIADVGADPRS
                     EGIDVVVTGHSHKPAISERDGVLFVNPGSAGPRRFKLPISAGMLIIEGGHASATVDFL
                     VT"
     gene            complement(3198..>4171)
                     /locus_tag="VHV99_10240"
     CDS             complement(3198..>4171)
                     /locus_tag="VHV99_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3635852.1"
                     /translation="KRRGFMGSFLEFGTLIGYVLGAGTVAVLTATLSNDALLSWGWRV
                     PFLIAGPLGLAGLYIRMKLEETPAFRKQAEQREAEDKALPKQSFRELLMQQWKPLLLC
                     VGLVLIFNVTDYMALSYLPSYLSATLHFNETHGLFLVLLVMVLMMPMTLAAGRLSDTI
                     GRKPVMLFGCVGLFALSIPALLLIRMGTVLPVFGGLMILGVLLSCFTGVMPSALPALF
                     PTKIRYGALAIGFNISVSLFGGTTPLVTAWLVDSTGNLMMPAYYLMGASLIGIVSVLA
                     LRETARKPLLGSGPCVATRAEAHAVLRDEREAAEMDEGYAAAATARA"
BASE COUNT          748 a         1278 c         1370 g          775 t
ORIGIN      
        1 gcgatccgca gcctcatccg atgaagccgg gcgaccacgt gctgatcgaa gcgcattgcc
       61 ggcatcgggt ggcatggacg agcggcacgg agccgagcgt gtggctagcg gtttactatc
      121 ctggcgttcg ccgctgattg agcgccggag cgcgccaggc ggaaggtacg gttgcttcga
      181 tcgacgaggc gatggattcg attgaaaagg ggaaaacatg agcattagca tcgtccagct
      241 ggggtctccg aggacgcccg acgaagggct gcgtatcggt accgttcggc gaccgccgcg
      301 cggagtaccc aggacggaat tcagcacgcg cgactattac gatgtatggc tgcctaatct
      361 gtcgcctgat gccgagttga tgaaagaagg aaagacggcc gccagcgacg ctgattgggc
      421 cgtgtttgcg aaaaaattcc gggcggagat gaatggtagc gacaccggca aggtgttgga
      481 cctgcttgcg gcattgtcaa aaacgacgca ctttgccatc ggctgctact gcgaggacga
      541 aagccggtgc catcgcggca ttcttcgcca gttgttaagt gagcggggtg cgctgataag
      601 gtgatcaagc tgtggatcgg tgtgaacgtt ccgctcgacg ggattgcgaa agcaccggcg
      661 aaatcaaggc ggggtgcccg catcgacaaa tcgcgcggtc atcgccgata attgccctcg
      721 ggcttgccag cgtcagcatg ccgacctgct gcgcgcggtg ctcgcgcgtc aggatcagat
      781 tcccggcaca cccgcattcc cctcacaacc gccgttgcac gaccaggaaa ccgaccgatg
      841 ggcaagggac gcgtcgaagc cttcagcgat ggcgtgatcg ccatcatcat cacgatcatg
      901 gtgctcgaat tgaaggtgcc ggaaggctac gatttcgccg cgttgcgccc ggtggcgccg
      961 gtgttttgcg cctacctgct gagtttcatc tacgtcggca tctactggaa caatcaccac
     1021 cacatgttcc atgccgtgca gaaggtcaat ggcgcggtgt tgtgggccaa tctccatctg
     1081 ttgttctggc tctcgctgct gccggcggtg acgcattggg tcggcgaaaa tcatctggct
     1141 tcgtggccga ccgcgatgta cggcgtcgtg ctgttcatgt cggcggtggc ctatttcatt
     1201 ctgacccgca tgctgatccg cgaacacggc aaggactcga cactggccaa agccgtgggc
     1261 aacgacttca agggcaaggt ctcggtcatc atctatctgt cgggcattgc gctggcattc
     1321 gttcagccgt ggctctcggc tgcgctctat acgctcgccg cggcgtggtg gctggtaccc
     1381 gaccggcgca tcgaacacgt gctggaagcg taagcatgct gcttgcgctc aagctcgcgc
     1441 tggtgcccgc gtttctcgcc gcgctgaccg tggcggggcg ggtgtggggg ccgtcggtgg
     1501 cgggatggct ggcggggctg ccggtgatcg ccgggccgat cgtgttgctg ctcgcgctgg
     1561 agcgtgggcc ggcgttcgcg gcgcaggctt cggcggcatc gatcgcggcg atcgccgcct
     1621 ccgaggcgtt caattttgcg tatacgtgga cctgccgacg ttcagcatgg ccgttttcgc
     1681 tcttggccgg catgctcggg tgggtcggcg tggcggtgct gctcacgcac ttgcccggcg
     1741 gattgccatg ggccgtggcg gtgggctgcg tggcggtgac cgtgtcgcaa agcggtttgc
     1801 cgcgcgtcac agggcatgtg ccggcggcgc gcctcgggct cggcgatctt gcgctgtgga
     1861 tgctcgccgg ggcactgctg acggtggcgg tgaccacgct gtcggcgtcg atgggtgcga
     1921 cgtggagcgg gctgctgtcg gtgtttccct tgctcggcat cgtgctcgcg gtgtcggctc
     1981 agcgcgcgca tggcgcggac ttcgtcgcgt tgttgatgcg aggcatggtg atcgggcgcg
     2041 gttcgtttgc ggcgttcttc gccgtgaccg cgatgatgct gccgcactat ggtgtgtggc
     2101 cgagctttgc gtgtgcggcg ctggtgtcgg tgattgtgca gggcgtgact aaacggatgc
     2161 ttgcggcgaa gcggccggtg caggctttga cgcgtgagtt tgcagaacaa cagtcggcgg
     2221 actgagaatt gagtgggtga acgcgcacac ctgctgaacg tctgcctccg actgcacgtg
     2281 taattctgaa gcagtcggcg gattgagaat cgagcgggtg aacgcgcgca cctgctgaac
     2341 gtctgcttcc ggacgtgtgg atttgaattt accgagcagg ccaagcgctt tgagcgtgcg
     2401 aactcgctaa cccagtgcag atggattggt cgtttgaact cgttgtgcaa acccaggtat
     2461 tttgagcgcg cgagttcgcc ggcccgccgc aaacagcaga ccaacgccgt ttggccgata
     2521 ctcgccctat gacttcgcgc acccaacgtt cctctgccac ccgaatcggc ctgatctccg
     2581 acacgcacaa cctcgtgcgt cccgaagcgc tgcagtatct ctcgggctgc cacgcgatca
     2641 ttcacgcggg cgacatctgc aatcaggcag tgctggatgc gctcgcgcgg atcgcgccgg
     2701 tcaccgccgt gcgcggcaac aacgacatcg acgagccggt cgccgcgctg ccgacacacg
     2761 tcaaactcac cgttcagcag gtgacgatcc tcgtcgtaca tgacatcgcc gacgtgggcg
     2821 ccgatccgcg cagcgaaggc atcgacgtag ttgtgaccgg gcactcgcat aagcccgcga
     2881 tcagcgagcg cgacggcgtg ttgttcgtga atccgggcag cgccgggccc cggcggttca
     2941 agttgccgat ttccgccggc atgctgatca ttgaaggcgg ccacgcaagt gcgacggtcg
     3001 attttctggt gacataaaaa agcgggccgg caaatttttt gccggcccgc tttcggaagc
     3061 gctgacgtga attgctgtgg ttgcctgccg ctaaccgcta accgccaacc gccaaccgcc
     3121 aaccagccaa ccgccaaccg cgactgcgga cggcacgcgt acaacgccgg cggctagcta
     3181 ccatgcaacc caggcgatca ggctcgcgcc gtagccgcgg cagcataacc ctcatccatc
     3241 tccgctgcct cacgctcatc gcgcagcacc gcatgcgctt ccgcacgcgt tgcgacacac
     3301 ggacccgagc cgagcaacgg cttgcgggcc gtttcgcgca gcgcgagtac cgacacgatg
     3361 ccgatcaacg acgcgcccat caggtagtac gcgggcatca tcagattgcc ggtgctgtcg
     3421 accagccacg ccgtgacgag cggcgtcgtc ccgccgaaca gcgacaccga aatgttgaag
     3481 ccgatcgcga gcgcaccgta gcggatcttg gtggggaaca acgccggcag cgccgacggc
     3541 atcacgccgg taaaacacga cagcagcaca ccgagaatca tcaggccgcc gaacaccggc
     3601 agcaccgtgc ccatgcggat cagcagcagc gccggaatcg acagcgcgaa cagacccacg
     3661 cagccgaaca gcatgacggg cttgcggccg atcgtgtcgg acaggcgacc ggctgcaagc
     3721 gtcatcggca tcatcagcac catcacgagc agtacgagga acaggccgtg cgtttcgttg
     3781 aagtgcagcg tggccgacag atagctcggc agatacgaga gcgccatgta atcggtgacg
     3841 ttgaagatca gcacgaggcc gacgcacagc agaagcggct tccattgctg catcagcaac
     3901 tcgcggaacg attgcttcgg gagcgctttg tcttcggctt cgcgctgctc ggcctgcttc
     3961 ctgaacgcag gcgtttcttc gagcttcatc cggatgtaca ggcccgccag tcccagcggg
     4021 ccggcgatca ggaacggcac acgccagccc cacgagagca gcgcgtcgtt cgacagcgtc
     4081 gcggtcagca ccgccaccgt gcctgcgcca agcacatagc cgatcagcgt gccgaactcg
     4141 aggaagctgc ccatgaagcc gcggcgcttg t
//
LOCUS       DATASH010000366         3529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1438030,
            whole genome shotgun sequence.
ACCESSION   DATASH010000366 DATASH010000000
VERSION     DATASH010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1438030"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2955
                     /locus_tag="VHV99_10245"
     CDS             <1..2955
                     /locus_tag="VHV99_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HEX3635853.1"
                     /translation="TKAPGLAPVDVERSITAPVELALQGLPGMQSLRSISRYGLSVIY
                     VRFADSTNLYLDRNLIGERLQTIQLPPGTSAPQMWPLSDGLSEIYQFQVRGHGQSLMT
                     LRTLLDWQIAPKLRQVEGVVDVNVNGGLLKTYEVQADDNALTRYGLSVGDIFNAVQMN
                     NGATGGATIEHNGGESIIRGEGLLRNTTDIGNIVLRTAAGGAPLYVHDVAHVDEAPMP
                     RLGAVTRDGKGEAVVGVVLMGIGQNTRVVAQRVQGAIKEINQTLPAGVEVVPYYNRAD
                     LMDRVLHTVAHNMVEGAVLVIAVLLLLLGNARAGLIVATAIPLSMLVAVMAMYGTGMS
                     GNLMSLGAIDFGLLVDGAVVMIENVVRRRSEAPNQPLRPLVLEAAREVSRPVTFAVLI
                     ITAVYLPILSLQGIEGKMFRPMAMTVIFALLASLVLTLTVMPVLASLLLRGKVAERES
                     RILHWVRLRYEPILARAAQQPALTMGLAVALLAVAAFAATRLGAEFLPRLDEGALTVT
                     TTKLPGISVPSAVEAQTMVERTLMRFPEVQSAVTLGGSSEIPTDPMGVEQSDTFIMLK
                     PQSEWKTAQTQDGLVTEYSAALDRAVPGLTLDWAQPIQMRMDDMIAGVQADVAVQIHG
                     NDLQTLHDTAERVAAVISRVPGAADVEAQQTIGQPYLRIIVKRDAVARYGLNASQVLD
                     LVQSLGGRNVGTIVDGEGQFDIRVRMRPEDREDVTRIGALRISNGDGRAVPLSELADI
                     RMEPGPSTIEREEGRRVILVQANVRGRDLASFVLAAQHAVQTQVRLPPGYTVSWGGSF
                     KNLQDAMARLYVVVPISLVTIFALLYLMFGSASLAALIFCNVPFAAIGGIFALWIRGL
                     PFSISAAVGFIALFGIAVLNGVVLVSSIVEMRARGLSAVEAAKQAAATRLRPVLMTAT
                     VASLGFLPMAFSMSSGAEVQRPLATVVIGGLVSATLLTLLVLPTVYPWFDRRKGAARL
                     AQVHEHETQFAE"
     gene            2972..>3529
                     /locus_tag="VHV99_10250"
     CDS             2972..>3529
                     /locus_tag="VHV99_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX3635854.1"
                     /translation="MTALKRLPVLLIPLLLAACAVGPDFRQPVAPSGADYTHEPLPSK
                     TAAAPGAAGGAQQFAVGGDIPGQWWTLFQSPELNAMIDRAFKASPTIAAAQSALAVAR
                     ENMLSQKGTLFPSIGVDASGSRNETSTASLAPVAANNQRIYSLYTGELTVSYSLDAFG
                     LNRRMIESAVAQADNQRFELEAAYLT"
BASE COUNT          613 a         1096 c         1152 g          668 t
ORIGIN      
        1 accaaggcgc ctgggctcgc gccagtcgat gtcgagcgct cgatcacggc gcctgtcgaa
       61 ctcgcgctgc agggcttgcc aggcatgcag agcctgcgct caatctcgcg ttacggtctt
      121 tccgtgattt acgtgcgatt cgccgactct acgaacctgt acctcgaccg caatctgatc
      181 ggtgagcggt tgcagacgat acagcttcca ccaggcacca gcgcgccgca gatgtggccg
      241 ctatcggatg gcctgagcga gatctatcag tttcaggtac gtggccacgg tcagtcgctg
      301 atgacgttgc gcacactgct cgactggcag atcgcgccga agctgcgcca ggtggaaggc
      361 gtggtcgacg tgaatgtgaa cggcggcctc ctgaagacgt acgaagtgca ggccgacgat
      421 aatgcgctca cgcgttacgg cctgtcggtc ggcgacatct tcaatgccgt gcagatgaac
      481 aatggcgcaa caggcggcgc gaccatcgag cacaacggcg gagaatccat catccgcggc
      541 gagggcttgc tgcgtaatac gaccgacatc ggcaacatcg tgctgcgtac cgctgcgggc
      601 ggcgcgccgc tttacgttca tgacgtagcg catgtcgacg aagcgcccat gccacggctc
      661 ggcgcggtca cgcgcgatgg caaaggcgaa gctgtcgtcg gtgtcgtgct gatgggcatc
      721 gggcagaaca cccgcgttgt cgcacagcgc gtgcagggcg cgatcaagga aatcaaccag
      781 acgctgccgg ccggcgtcga ggttgtgccc tactataacc gcgcggacct gatggaccgg
      841 gtgctgcata cggtcgctca caacatggtt gaaggtgccg tgctcgttat cgcggtgctg
      901 ctattgctgc ttgggaatgc acgcgcaggg ctgatcgtcg ccacggcgat cccgctgtcg
      961 atgctcgtcg ccgtcatggc gatgtacggg acagggatgt cagggaatct gatgagcctc
     1021 ggtgcgatcg atttcggcct gctggtcgac ggcgcggtgg tgatgatcga aaacgtcgtg
     1081 cgacggcgtt ccgaagcgcc caatcagccg ttgcgcccgc tcgtactgga ggcagccagg
     1141 gaagtgtccc ggcctgtaac gttcgcggtt ctcatcatca cggccgtcta ccttccgata
     1201 ctgagccttc agggtatcga aggcaaaatg ttccggccga tggcgatgac cgtgatcttc
     1261 gcactactcg cgtcgctcgt cctcacgctg acggtcatgc cggtgctcgc tagcctgttg
     1321 ctgcgcggca aggtcgcaga gcgtgaaagt cgcatccttc actgggtacg cctgcgctat
     1381 gaaccgattc tcgcacgtgc cgcgcaacag cctgcgctga cgatggggct cgccgtggcg
     1441 ttgctcgcgg tggcggcctt cgccgccacg aggctgggtg ccgaattctt gccacgcctc
     1501 gacgaggggg cgctaaccgt cacgacgacg aagctgcccg gcatatccgt gccgtccgcg
     1561 gtagaggcgc aaaccatggt agaacgcacg ttgatgcgct ttcccgaagt gcagtccgct
     1621 gtcacgctcg gcggcagctc ggagattccg accgacccga tgggcgtgga gcaaagcgac
     1681 accttcatca tgctgaagcc gcagtccgaa tggaagacgg cgcaaaccca ggacggcctc
     1741 gtcaccgagt actccgcggc gctggatcgc gcagtcccag gcctgacgct cgattgggcg
     1801 caaccgatcc agatgcgcat ggacgacatg atcgcgggcg ttcaggccga tgttgccgtg
     1861 cagattcatg gcaacgatct gcaaacgctg cacgataccg ccgagcgggt tgcggccgtg
     1921 atctcacggg tgccgggtgc ggcagacgtc gaggcgcagc agacgattgg ccagccttac
     1981 ctgcggatca tcgtcaagcg cgacgcggtg gcgcggtacg ggctgaatgc gagccaggtg
     2041 ctcgatctcg ttcagtcgct gggcggccgc aatgtcggga cgattgtcga cggtgaaggc
     2101 cagttcgata tccgggtgcg catgcggccg gaagaccgcg aggatgtgac gcgcatcggc
     2161 gcgctacgca tcagcaatgg cgatggccgg gccgtccccc tgtcggagct ggcagacatc
     2221 cggatggagc cgggaccgtc gaccattgag cgtgaggaag gcaggcgcgt catcctcgtt
     2281 caggccaacg tgcgcggacg cgacctcgcc agcttcgtcc tcgctgcgca acacgcggtt
     2341 cagacgcaag tcaggttgcc gcctggctac accgtgtcgt ggggcggcag cttcaagaac
     2401 ctgcaggacg cgatggcgcg cctttacgtc gtggtgccca tctcgcttgt cacgatcttt
     2461 gcgttgctct acctgatgtt cggcagtgcc agtcttgcag cgctcatctt ctgcaacgtg
     2521 ccgtttgcag cgatcggcgg gattttcgcc ctctggattc gcgggctgcc gttcagtatc
     2581 tccgctgcgg tcggctttat cgcgctcttc ggtattgccg tgctcaacgg cgtcgtgctg
     2641 gtcagcagca ttgtcgagat gcgggctcgc ggcctgagtg ccgtcgaggc ggcgaaacag
     2701 gccgccgcga cccggctgcg gcccgtgctg atgacggcaa ccgtagccag ccttggcttt
     2761 ctgcccatgg ccttctcgat gagctcgggt gcggaagtcc agcgtccgct tgcgacggtc
     2821 gttatcggtg gccttgtgtc ggcaaccttg ctaacgctgc tggttctgcc gaccgtttat
     2881 ccgtggttcg atcgccgcaa aggcgcggcg cgtcttgcgc aggtgcacga gcacgaaacg
     2941 caattcgcag aatgatgagg gatgctcaac cgtgactgcc ctaaaacgtt tacccgtcct
     3001 gttgataccg ctactgctgg cggcttgcgc ggtaggtcct gacttcaggc agccggtcgc
     3061 cccgagcggc gcggattaca cgcatgagcc gctgccgtcg aagaccgcag cggcccccgg
     3121 cgcagcggga ggggcgcagc aattcgctgt tggcggcgat attccgggcc aatggtggac
     3181 gctgttccag tcgccggagc tcaacgcgat gatcgaccgc gcgttcaagg ccagcccaac
     3241 catcgctgcc gcgcagtctg cgctcgccgt ggcccgcgag aacatgctga gccagaaggg
     3301 gacgctcttt ccgagcatcg gcgtcgacgc gtcaggcagc agaaacgaaa cgtccacggc
     3361 gagccttgct cccgtcgccg ctaacaatca gcgcatttac tcgctctaca cgggcgagct
     3421 gaccgtgtcg tattcgctcg acgcattcgg cttgaatcgc cgcatgatcg aatcggcggt
     3481 cgcgcaagcc gacaatcagc gtttcgaact ggaagcggcc tatctgacc
//
LOCUS       DATASH010000367         5904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_52498,
            whole genome shotgun sequence.
ACCESSION   DATASH010000367 DATASH010000000
VERSION     DATASH010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5904
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52498"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(745..1872)
                     /locus_tag="VHV99_10255"
     CDS             complement(745..1872)
                     /locus_tag="VHV99_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEX3635855.1"
                     /translation="MTNSPVNILIVDDIVHNITALEALLARPDLAVLVADSGTAALDL
                     LLKHEVALAILDVNMPGMNGFELAALMRGSPRTSHVPIIFLTATAQDASRTFRGYEAG
                     AVDFLYKPFDPRILQSKVDVFVQLEQQKRQLAAQLVTTRQMLEANEMLMAVLSHDLRT
                     PLGAVLASAEYLMRTATDDQSMTVATRVKNSSLRMARMVDQLLNLARLQGGRLPLQPR
                     SIELATLCRSVIDEFASRENGKRIVFASRGNTAGAWDTDLVWQAVSNLVSNALHHGTQ
                     GGDIGVEVDGDSVESVRLKVANRGTIPPEVFPHLFKAFGPNSSGARSREGLGLGLHIV
                     QEIARMHGGSVSVSSDEAVGTVFVIELPRMVTLVRGSFTGR"
     gene            complement(1882..2508)
                     /locus_tag="VHV99_10260"
     CDS             complement(1882..2508)
                     /locus_tag="VHV99_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEX3635856.1"
                     /translation="MSRSTATPAIESQPGAAEARGFELVVIGGSAGGIEVLNVLLGAL
                     PASFAPAVMIVTHLPPDSPSYLVHAFAHRCALPVLEPDAGERIMPGRVHVAPPGYHML
                     VEVDRTVALSTDAAVRFSRPSIDVLFDSAASVYGERLLAILLSGANDDGARGMKRVRA
                     LGGTAWVQAPDSASSPEMPRAAIERGAVDHIYSPKTMARRLAALPASL"
     gene            complement(2519..3364)
                     /locus_tag="VHV99_10265"
     CDS             complement(2519..3364)
                     /locus_tag="VHV99_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEX3635857.1"
                     /translation="MSQSEFDVPQRISDFDIELKLLLEAIYLKYQHDFRHYAMSSLRR
                     RLSQALEEFGLRTLSQLQDRIMRNGDEFSRLFQYLTVQVSDMFRDPSYFLALREHVLP
                     RLRTYPSIKVWVAGCSTGEELWSLKILFDEEGLTERTLFYATDINPDALARAESGIYA
                     LDRIQGFTQNYLAAGGKRSLSDYYHAAYGGARFAGSLKDRVVFADHSLSTDEVFLEAH
                     LVSCRNVLIYFDRGLQDRALGLFENALVRRGFLGLGSKESLRFSNHSEAFDEFRPSER
                     IYQKR"
     gene            complement(3383..>5904)
                     /locus_tag="VHV99_10270"
     CDS             complement(3383..>5904)
                     /locus_tag="VHV99_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3635858.1"
                     /translation="LIMPIENDGQVNGVVELGFIRQLTARDQEFMNLISNSMGDFVEA
                     ALYRERLQDALVETQQLNEELQVQQEELRVSNEGLEERGRALMESQTRLEAQQAELEQ
                     TNVQLEEYAQRLERQKSDLLRAQDDLAANAARLEQSSRYKSEFLANMSHELRTPLNSS
                     LILAKLLQQNRTGNLNEEQVRYAETIHASNSDLLVLINDILDLSKVEAGQVTVELETV
                     SVDATLQSLQEMFSPMAAAKHLSLAFDRLPGTPDTFITDGQRVSQILRNLLSNAVKFT
                     EHGEVAVSAAPAEGNMLRIDVRDSGIGIPADKLEMIFEAFQQADGSTSRQYGGSGLGL
                     SISREFSRLLGGRITVASEVGKGSVFSLWLPLDAEAALAGSRNVQMPAPAFAQNHAPA
                     TAASPVRAAPEPLLTRTPSNGAQPAVITAANGLSEGNAPEATSGPIADDRDNRTRAGR
                     LIVAIEDDPAFAEILRELTHELDFDFVHASSAATGLALVRDMCPAAVLLDVGLPDRSG
                     LTVLEWLKNDPLTRHIPIHIVSATDHADKALHLGAIGYTLKPTARSTMEAAIRRLEAR
                     LQQRLKRVLVIEDDAPMRESIRALLQNESTEIVAVATLAEALEYLAKFTFDCVVTDLA
                     LPDGTGYDLLERLAANTAHAALPVIVYTGRMLSDEEEHRLRRYSKSIIIKGAKSPERL
                     LDEVTLFLHSVESSLAPEQQRMLRTVRQRDNAFEGRTILLAEDDVRNIFALSHVLEPL
                     GAKLEIARNGREALEALENGPEVHLILMDIMMPEMDGLTAMREIRRNPRFAHLPIIAL
                     TAKAMANDRTRCLEAGADDYVSKPIDVDKLVSLCRVWLRQR"
BASE COUNT         1173 a         1896 c         1772 g         1063 t
ORIGIN      
        1 ggctcacacc gccagcccat aagcgggtcc cccgcgcagt cgcggtagtg gtgcatctgg
       61 aatccgtgcc atcgcacatt cggcgtgagt gaccaggccg tcatcagcgc ccactccgca
      121 ctgcgtgcgt cgcggatggg tctgcatggg aaatcagggg cttcgttcac acgcggtgga
      181 gccgtcggct tcgcctcggc caagcgccca gaatccgaga aatgcgctcc gcaaccaaca
      241 agaagcgcga atacactgcc caaatgaaac gctgcggatt gtgtttgtag tggcaatcgt
      301 agctgcggtt gctgtgccct tgctgtgtca gtgacgatcc aactgcgcgc cgtggtgcca
      361 agcagaacgc gagcgcgagc gcgcagcgat cggtgttgtg aaatgcgctt cggcgcgcgc
      421 agcgccgccg gaagtatgac gcctttgtca ctcacgcgga gtgcgcgaga acaccgattc
      481 cagatgcacc actaccgcga ctgcgcgagg gacccgctta tgagctggcg ggttgagccg
      541 ctttctttgc ttatctttct ttgcggcggc aaagaaagta agtgccgccc cgcacagggg
      601 caacgctaat agaccactat caattcaagg aaaggccaaa aagccagatc aaggaaaggc
      661 cacgccgtag gcacacaaac cccaagcgcc gcgcaggcaa aaaaatcagg aaaaccccaa
      721 cgccgcaggc aaacaaacac ccctttatct cccagtaaaa gaccccctca ccaacgtcac
      781 catcctcggc aattcaataa caaaaacagt accaacagcc tcatcagagc taacactgac
      841 actcccgccg tgcatgcggg caatctcctg cacgatatgc aacccaagcc ccaacccctc
      901 ccgcgaccgc gctccactgc tattcggccc gaaggccttg aaaagatgcg gaaacacttc
      961 aggcggaatc gtcccacgat tggcaacctt caaccgcact gactcgacgg agtccccgtc
     1021 cacctcgaca ccgatatccc cgccctgcgt gccatgatgc aacgcattac tcaccagatt
     1081 cgacaccgcc tgccaaacca gatccgtatc ccacgcaccc gcagtgttcc cccggctcgc
     1141 aaacacgatc cgcttgccgt tctcccgcga agcgaactca tcgatcaccg atctgcacaa
     1201 cgtcgccagt tcgatcgagc gcggctgcaa cggcaagcgg ccaccctgaa ggcgcgcaag
     1261 attgagcaac tgatccacca tccgcgccat gcgcaacgaa ctgttcttca cgcgcgtcgc
     1321 caccgtcatc gattgatcgt cggtcgcggt gcgcatcaga tattccgccg acgccagcac
     1381 cgcgcccagc ggcgtgcgca aatcgtgact cagcaccgcc atcaacatct cgttggcttc
     1441 aagcatctgc cgcgtcgtga cgagctgcgc cgcaagctgg cgcttctgct gctcgagttg
     1501 cacgaacaca tcgaccttcg attgcagaat ccgcggatcg aacggcttat aaaggaaatc
     1561 gaccgcgccg gcctcgtagc cgcgaaacgt gcgcgatgca tcctgcgcgg tcgcggtcag
     1621 aaagatgatc ggcacatgcg aagtgcgcgg gctgccgcgc atcaaggcag ccagttcgaa
     1681 gccgttcatg cccggcatgt tgacatcgag aatcgccagc gccacctcgt gcttgagcag
     1741 cagatcgagc gccgcagtgc ccgaatccgc caccagcacg gcaaggtccg gccgcgccag
     1801 caaagcttcg agcgcggtaa tgttgtggac gatgtcgtcg acgatcagga tgttcacagg
     1861 tgagttcgtc atggcatcgg gtcagagaga ggccggcaat gctgcaagcc gccgggccat
     1921 ggttttggga gaatagatgt gatccacggc accgcgctca atcgcggcgc gcggcatttc
     1981 gggcgaactg gcgctgtcgg gcgcctgcac ccaggccgtg ccgcctaacg cccgcacgcg
     2041 cttcatgccg cgcgcgccgt cgtcgttggc gccggacagc agaatcgcca gcaaacgctc
     2101 gccatatacc gaggcggcgg aatcgaacaa cacatcgatc gacggccgcg aaaaacgtac
     2161 ggcggcatcg gtcgaaagcg cgacggtgcg gtccacttcc accagcatgt gatagccggg
     2221 cggcgcgaca tgcacgcggc ccggcatgat ccgctcgccc gcgtcgggtt cgagcacggg
     2281 caacgcgcag cgatgcgcga acgcgtgcac caggtagctc ggcgaatccg gcggcaggtg
     2341 ggtgacgatc atcaccgccg gcgcaaagct cgcgggcagc gcgccaagca gcacgttcaa
     2401 cacttcaatg ccgccggctg agccgccgat caccacgagc tcgaagccgc gtgcttcggc
     2461 agcacccggc tgggattcga tggctggcgt ggcggttgag cgggacatga tggccgtgct
     2521 agcgcttctg ataaatgcgt tcgctcggac ggaactcgtc gaacgcttcg gaatggttcg
     2581 aaaaacgcaa actttccttg ctgcccaagc cgagaaaacc gcgccgcacc aacgcgtttt
     2641 caaacagacc cagcgcgcga tcctgcaagc cgcgatcgaa atagatcagc acgttgcggc
     2701 acgataccag gtgcgcctcg agaaagactt cgtcagtgga caggctatgg tcggcgaaaa
     2761 caacgcgatc cttcagcgaa cccgcgaagc gcgcgccacc atacgccgca tgatagtaat
     2821 ccgataacga ccgcttgccg ccggccgcca ggtaattctg cgtgaagccc tgaatccgat
     2881 cgagcgcata gatgccggac tcggcgcgcg ccaatgcgtc agggttgatg tcggtcgcgt
     2941 aaaacagcgt gcgttccgtc aacccctctt cgtcgaagag gattttcagc gaccataatt
     3001 cctcgcccgt gctgcacccc gctacccaca ctttgatgga cggataggtg cgcagacgcg
     3061 gcagcacatg ttcgcgcagc gcgaggaaat acgacggatc gcggaacatg tcgctgacct
     3121 gcaccgtcag gtactggaac agccgcgaaa actcgtcgcc gttgcgcatg atgcggtcct
     3181 gcaactgcga cagcgtcctc agcccgaact cctcgagcgc ctgtgacagc cggcggcgca
     3241 gcgacgacat cgcgtaatgc cggaaatcgt gctgatattt gaggtaaatc gcctccagca
     3301 gcagtttcaa ctcgatgtcg aaatcgctga tgcgctgcgg cacgtcaaac tcggactggc
     3361 tcatcgtgtt cccgtttccc cactaccgtt gccgcagcca cacacggcac agcgaaacca
     3421 gcttgtccac gtcgatcggc ttggacacgt agtcgtcggc accggcttcg aggcagcggg
     3481 tacggtcgtt cgccatcgcc ttggcggtca gcgcaatgat cggcagatgc gcaaagcgtg
     3541 gattgcggcg gatctcgcgc atggccgtca atccgtccat ctccggcatc atgatatcca
     3601 tcaggatcag atgcacttca ggaccgtttt ccagtgcctc gagcgcttcg cgtccattgc
     3661 gcgcaatctc cagtttcgca ccgagcggct ccaatacgtg tgagagcgca aaaatattgc
     3721 gcacgtcgtc ttcggccagc aggatggtgc gcccctcgaa ggcattgtcg cgctgccgca
     3781 cggtgcgcag catgcgctgc tgctcgggtg ccagcgacga ctccacgctg tgcaggaaca
     3841 gcgtgacttc atcgagcaga cgctcgggtg acttcgcccc cttgatgatg atcgacttcg
     3901 aatagcggcg caggcggtgc tcttcctcgt cggacagcat gcggcccgtg tagacgatca
     3961 ccggcagcgc ggcgtgcgcg gtgttcgcgg ctagccgttc gagcagatcg tagccggtgc
     4021 cgtcgggcag cgcgaggtcg gtcaccacgc aatcgaatgt gaacttcgcc agatattcga
     4081 gcgcctccgc caaggttgca accgcaacga tctcggtgct ttcgttttgc agcaacgcgc
     4141 ggatgctttc gcgcatcggc gcgtcgtcct cgatcaccag cacgcgcttg aggcgctgtt
     4201 gcaggcgggc ttcgagacgg cggatcgcgg cttccatcgt gctgcgcgcg gtcggcttga
     4261 gcgtatagcc aatggcgccg aggtgcagcg ccttgtcggc gtggtcggtg gccgaaacga
     4321 tatggatcgg gatgtggcgc gtgagcggat cgttcttcag ccactccagc acggtcaggc
     4381 cggagcggtc gggcaagccg acatcgagca gcaccgcggc cgggcacatg tcgcgcacca
     4441 gcgcgaggcc ggtggcggcg ctgctagcgt ggacgaaatc gaaatcgagc tcgtgcgtca
     4501 gctcgcgcag gatttcggca aaggcgggat cgtcttcaat ggcgacgatc agccggccgg
     4561 cgcgcgtgcg gttgtcgcgg tcgtcggcaa tcgggccgct ggtggcttcg ggtgcattgc
     4621 cctccgacaa gccgttggca gccgtgatga cggccggttg ggcgccgttc gagggcgtgc
     4681 gcgtcagcag cggctccggc gccgcccgga ccgggctcgc tgcggttgcg ggcgcgtgat
     4741 tctgtgcaaa cgccggcgcc ggcatttgca cattgcggct gcccgccaat gccgcctcgg
     4801 cgtcgagcgg caaccagagt gagaacacac tgcccttgcc cacttcgctg gccactgtaa
     4861 tccggccacc gagcagacgg gagaactcgc gcgaaatgga caggcccaaa ccactgccgc
     4921 cgtattggcg gctggtggat ccgtcggctt gctggaatgc ttcaaagatc atttccagct
     4981 tgtccgcggg aatgccgatg ccggaatcgc gcacgtcgat acgcagcata ttgccttcag
     5041 ccggcgcggc cgacacagcc acttcgccgt gttcggtgaa cttcacggcg ttcgagagca
     5101 ggttacgcag aatctgcgag acccgctggc cgtcggtaat gaacgtgtcg ggcgtgccgg
     5161 gcagacggtc gaaggcgagg ctcaagtgct tcgcggcggc catcgggctg aacatttcct
     5221 gtaacgactg cagggttgca tcgaccgata cggtttcgag ttcgaccgtc acctgtcccg
     5281 cctcgacctt cgacaaatcg agaatgtcgt tgatcagcac cagcaaatcg ctgttcgacg
     5341 cgtgaatggt ttccgcgtag cgcacctgct cttcgttcag attgccggtg cggttctgct
     5401 gcaacagctt cgcgagaatc aacgaactgt tcagcggagt acgcaattca tgcgacatgt
     5461 tcgcgaggaa ctcggacttg tagcggctcg actgctcgag gcgcgctgcg ttggcggcga
     5521 gatcgtcttg cgcgcgcagc aggtccgact tctgccgctc gagacgctgc gcatattctt
     5581 cgagctgtac gttggtctgc tcgagttcgg cctgctgcgc ctcgaggcgc gtttgcgact
     5641 ccatcagcgc gcggccgcgt tcttccaggc cctcattgga gacgcgcagt tcttcctgct
     5701 gaacctgaag ctcttcgttc aactgttgcg tctcgacaag tgcatcttgc agacgttcgc
     5761 gatacagggc cgcttcgacg aaatcgccca tgctattgct gatcagattc atgaactcct
     5821 gatcgcgcgc cgtcagctgg cggataaagc cgagttcgac gacaccgttg acctggccgt
     5881 cgttttcgat cggcatgatc agca
//
LOCUS       DATASH010000368        12326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4999881,
            whole genome shotgun sequence.
ACCESSION   DATASH010000368 DATASH010000000
VERSION     DATASH010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 12326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12326
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4999881"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VHV99_10275"
     CDS             complement(<1..263)
                     /locus_tag="VHV99_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX3635859.1"
                     /translation="MRTDQTFTTAREELVLAVSLELASVKWKVALHDGRREKPAVHTV
                     AQPQAAARLQAVLSLIEAHKGKWSLPEGVRVVVIYEAGQDAFW"
     gene            complement(484..1218)
                     /locus_tag="VHV99_10280"
     CDS             complement(484..1218)
                     /locus_tag="VHV99_10280"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX3635860.1"
                     /translation="MTRFPYRDKTADDAFCGNSVRNDWQEERTALLNRTRGLLAEFGV
                     VIARSADRFLGALPALIEDPRLPDPVREQLAALNLRLARCDQQIASHVRSSDVERRAR
                     ELPGIGPVTSSAREAMVPDAKVFRNGRQFGAWLGLTPRQHSSGGRTRLGHFSLRGDVY
                     PRTLPVQGARSTLQSALHADHTHASRLQRWIGQLHELKGYHKTLIAIANKHARMLWAM
                     LAKNERYDADAWQRHPMYPPLAPAAA"
     gene            complement(1276..1416)
                     /locus_tag="VHV99_10285"
                     /pseudo
     CDS             complement(1276..1416)
                     /locus_tag="VHV99_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012606398.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            complement(2351..2791)
                     /locus_tag="VHV99_10290"
     CDS             complement(2351..2791)
                     /locus_tag="VHV99_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HEX3635861.1"
                     /translation="MRRVIVRRSPVHGKGVFAMHALAAGERVLEYRGEITSWRNAVRR
                     HQREGVEGHTFLFGLSDGRVIDGSRGGNSARWLNHACAPNCETIEDGGRIFILTLRPI
                     GAGEELFIEYLLATEDPLDEDVRAQYACRCAAADCRHSMLADVA"
     gene            complement(2975..3181)
                     /locus_tag="VHV99_10295"
     CDS             complement(2975..3181)
                     /locus_tag="VHV99_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635862.1"
                     /translation="MEKKRSRTGREISCPTIGALLRYRTRIVRVIAEARGQRAMIESL
                     DTTGRTFVSTVKWVSLRELGNQLF"
     gene            complement(3387..3665)
                     /locus_tag="VHV99_10300"
     CDS             complement(3387..3665)
                     /locus_tag="VHV99_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX3635863.1"
                     /translation="MNKQELVDAVAAKTGDSKVATGEMIDAVIAAITGELTKGKTVQL
                     IGFGSFSTGARAARVGRNPATGAEIQIPAAKTVKFTAGKAFKDAVNGA"
     gene            complement(5111..5893)
                     /locus_tag="VHV99_10305"
     CDS             complement(5111..5893)
                     /locus_tag="VHV99_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HEX3635864.1"
                     /translation="MSHDATIERLYVLDAGIAQVDDASIYSPGFNVGKPMTLSCNAYL
                     IRHRGQWMLWDTGTQDDLIGEPEGRIIAHGIRGTISKTIASQLQEIGVEPDEIGTLAL
                     SHAHYDHVGNCRLFTKAEWIVQKAEYEAMFGADPDAFGFRPELYGTLRNNRLRLIEGD
                     HDVYGDGAVRIIFTPGHTPGHCSLLLNLPEAGRVLLSGDVAHNRRNFQCRCVPSFNVD
                     AVQSIASMNRVEELLETEGATLFVNHDVVQNATLPHAPDWLA"
     gene            complement(5908..6834)
                     /locus_tag="VHV99_10310"
     CDS             complement(5908..6834)
                     /locus_tag="VHV99_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX3635865.1"
                     /translation="MKNVNPNLEVGELGLKLANLRMDGALALGAELDGRIVNITQAGA
                     KFEMPAPLDMDDLLQNGRAADVSAIVNRLRQDGGIDSVEVREAAFAPLVTRPQKIICV
                     GFNYQAHAAETGTPIPKAPPIFSKFANALNHHEGDIELPAMVDKEFDYETELVIVFGR
                     KCKNVSKENAQSVVAGYAVGNDFSARGLQNITTQFTAGKMSDGFAPLGPWLATRDRVP
                     EPNNLRLQTWVNGEIRQDSNTGEMIFDCREIIRYVASIMTIEPGDILFTGTPPGVIWG
                     QKVPRDERQWLKAGDKVVSAIEGLGELAIHLR"
     gene            complement(7015..7614)
                     /locus_tag="VHV99_10315"
     CDS             complement(7015..7614)
                     /locus_tag="VHV99_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEX3635866.1"
                     /translation="MELPPEAAGDHDELNQEFGRRMRALRERGELTLEELATRSGVSR
                     AMLSKVERGEKSPTIGVAKRIAHALETTLSFLTGGVEDRQAVVVVRREQRHIFRDSET
                     GFERHILSPTMVGSQVELLQHWLPAGVSTGMMPAYRTGTEKHVTVTEGELIVSLPDQE
                     IRLSEGDTLFFEARVEHAFSNRSRKHCSYYLVISRRDDK"
     gene            7865..9259
                     /locus_tag="VHV99_10320"
     CDS             7865..9259
                     /locus_tag="VHV99_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012218442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX3635867.1"
                     /translation="MTQAIDALFEQDRAHFMHPSTHAHDHQSGALPGRIVRGGSGIHI
                     EDHEGRRYIDAFAGLYCVNIGYGRTEVADAMYEQAKQLAYYHTYVGHSSDVIIELSSR
                     LMEWAPPGMKKVYYGMSGSDANETQIKIVWYYNNVLGRPNKKKIISRERGYHGSGIVT
                     GSLTGLPSFHQHFDLPIERVKHTVCPHWYRNAPVGMNEAAFVAYCVDELEKLIAREGA
                     DTIAAFIGEPVMGTGGIVPPPAGYWPAIQQVLKKHDILLICDEVVCGFGRLGSKMGAQ
                     HFGIQPDLITVAKGLTSAYAPLSGVIVGEKVWDVIARGSQEHGPMGHGWTYSGHPVCA
                     AAALANLTIIEREKLVENAAQVGSYFGAQLREAFGSHPLVGEVRSTGMLAALEFMADK
                     EARKPFDPALKIGPRVAAAALQRGLIARAMPHGDILGFAPPLVMRRDEADEIVGIVKQ
                     AVDEVAERALADAG"
     gene            9316..10419
                     /locus_tag="VHV99_10325"
     CDS             9316..10419
                     /locus_tag="VHV99_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004190757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3635868.1"
                     /translation="MPKTQEVIVSFRGVRKTYDGESLVVKSLDLDIYRGEFLTMLGPS
                     GSGKTTCLMMLAGFEFPTGGEIHLDGKLLNNVPPHKRDIGMVFQNYALFPHLTVGQNV
                     AYPLSVRKVPATERAARVNEALRMVRMESFEKRYPAQLSGGQQQRIALARALVSKPKL
                     VLMDEPLGALDKQLREHMQYELKSLHAELGLTFVYVTHDQDEALTMSDRVAVFDKGVV
                     QQLDTVDRMYESPCNEFVANFIGDSNRLRGTIASVDGDFCDFRLDDGTRLVGRNIGKA
                     AAGASGIACIRPERMNVVYGSDRANDAAANALAGEARSLIYFGDHVRMRCAFKGQDEC
                     FVKVPIGTGALERFAPGAPVTLAFAPEHLRVFA"
     gene            10489..11538
                     /locus_tag="VHV99_10330"
     CDS             10489..11538
                     /locus_tag="VHV99_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006753702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3635869.1"
                     /translation="MITRAKLAAPCTVLALTLVMSGAPASAAELTVVNFGGSWGQVQN
                     IAFNQPFEKATGNKVTVVEYNGEQARVKAMVEAKHVNWDVVEVESGELNRGCDEGLYE
                     QLDWSKIAKKSDLVPEAPQTCGVGFVVWSMALAYNADKLKTAPTSWADFWDVKKFPGK
                     RAMRKGARYNLEFALMADGVAPKEVYKVLATKTGQDRAFKKLGELKPYIQWWEAGAQP
                     PQFLVAGDVVMSTSFNGRIDAAQREGQNLKVMWNGSIYDLDYWVIPKGTPNKALAEQY
                     IAYTLTPKPQVAFTQHIAYGPADVVALKSLDAKTLANLPNSPTNAKDATLQNIGFWTD
                     HGDELEQRFAAWASK"
     gene            11631..>12326
                     /locus_tag="VHV99_10335"
     CDS             11631..>12326
                     /locus_tag="VHV99_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006404050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3635870.1"
                     /translation="MNTMTIAPSSSTLSTAALKRELRSAGARKRAMALLLIAPLAIFV
                     LLIFVVPIGALLTRAVQNPEIATALPNTVATLSGWDRHAPPPDAAYAALAADMTKLAG
                     SDEMGTLASRLNLEIPGFRSFVAKTVRAMPLKGSSDQPLTPAQTREKLIEFDARWGDA
                     TYWQAIAKNGSQISPFYLLAALDHRQDGFSHIVHADPEQSIYLTIFGRTFLIGMAVTL
                     FALLLGYPLAYWVS"
BASE COUNT         2448 a         3863 c         3596 g         2419 t
ORIGIN      
        1 atccagaacg cgtcctggcc ggcctcgtag atcacgacaa cgcgcacacc ctccggcaac
       61 gaccacttcc ccttgtgcgc ctcgatcagg ctcagcacgg cctgcaggcg cgctgcggcc
      121 tgcggttggg ccaccgtgtg caccgccggc ttctcccgcc ggccgtcgtg aagcgcgacc
      181 ttccacttca ccgaagccag ctcgagtgac accgccagca ccaattcctc acgagccgtc
      241 gtgaacgtct ggtctgtgcg catgatgctc tccttgtagc agggttcgac cgactgattt
      301 tatcgatcag cgtcggcaga tttcctgatg cataggatct atttgaaaac cggcgaacag
      361 acactcagcg atgcaccgcg tgcggtgatg actcgccaac tgaatcggcg ggtttttctc
      421 ccgttcggtc cgaccttgtc ttgctatcgc gtcgagcagt agttgagttt caagacaatc
      481 gggtcatgct gctgcgggtg ctagcggcgg atacatggga tggcgttgcc acgcatcggc
      541 gtcgtagcgt tcgttctttg caagcatcgc ccacagcatt cgggcatgct tgttggcaat
      601 tgcaatcagc gtcttgtgat agcccttgag ttcgtgaagc tgcccgatcc accgttgcag
      661 ccggcttgcg tgcgtgtggt cggcatgcaa cgccgactgc aatgtgcttc gggcgccctg
      721 aacgggcagt gtgcgcggat agacgtctcc acgcaggctg aaatgcccca gccgggtcct
      781 gccgcccgag ctgtgctgtc gcggcgtcag gcccagccag gcgccaaact gtctgccgtt
      841 tctgaagacc ttcgcgtcgg gcaccatggc ctcgcgcgcg cttgaggtca ccgggccgat
      901 accgggcaat tcgcgggcgc gtctttcaac gtcgctggat cggacgtgag aggcgatctg
      961 ctggtcgcag cgagcgagcc tcaggtttag cgcggccaac tgctcgcgca ccggatcagg
     1021 cagtctgggg tcttcgatca gggcgggcaa tgcgccaaga aaccggtctg cagaacgagc
     1081 aattaccacg ccgaattctg ctagcagccc gcgcgtgcgg ttcagcaatg ccgtgcgttc
     1141 ctcctgccag tcattgcgca cgctattgcc acaaaacgca tcgtcggcgg ttttgtcgcg
     1201 gtaaggaaag cgggtcaagc aaatgtgata tgcgaaggat agcttacgaa atcaacgagt
     1261 aaaattgctg ggcggctttt cgaacagtgg caccaccttg atgacccagc gatgcaccgt
     1321 cgagtgatcg acacaaatgt cgcgctcggc catcatctcc tcgagattgc gaagactcag
     1381 cgagtaggca acgtaccatc gcacgcacaa caggattact tcaagcggat agcggggcga
     1441 ataaggcgct caagcacgcc ctgttgactg gctgtcgcgc aagaagaagg cagagaagcg
     1501 tcgaataccg gcagcacccg aatcgattga tgtgctttcg gtgacgcaag gttcaattgc
     1561 ggctgcttcg tcggactgca ggcatggaac aatcgatacc tccaccggct ttttgcgtgc
     1621 acaaaactgc agaagcgggc cgcgagctac ggcttgcgcc ttagaaaggc agatcccagc
     1681 taccttaacg cgctcagttg caacgactgt ccggttcgag gcattaacgg gaatcggcaa
     1741 gtgattgcta gtgaccgaac tctgtccgat tcgaagcgct cgcgagaatt atcgaaggtt
     1801 cggcatctgc acctagaaat gccatcgaaa aatcatggtt ccaaccgcgg tcaagaaaac
     1861 agtggcaagg tttcgacggc ggtttggcgc cgacgccgcg gcgacgcaga tcgactggcc
     1921 cgtgcagcag ttttcccggt agctgaaccg agccgccggt tcagatccga cagcgcccga
     1981 cagaccgacg tgcgagcggg cgttcccgct aatgccagtt caagctggtc cagcagttcc
     2041 ggcagaccca ttctccctga tctctttcgt gcgaacgtcc gcttctgctt taggccgctc
     2101 ccctatagct gtcgacatac gcgctggcat cgacgttacc accaatcgtc aagctccagc
     2161 gacatctgtt tcttcccgtt tgcgccggga gcaccgtcgg atacatttct ctttggcaac
     2221 ggtggcttca gcacatcgag atggaaaacc acatccacga cgagccggac aaatccatca
     2281 ttctcccgct tcggactccc ggcaactttc gggatcgtga tgccgccaac tggctctccc
     2341 gccccactgt tcacgctacg tcagccagca tcgagtgacg acagtccgcc gcggcgcacc
     2401 gacacgcgta ctgcgcgcgg acgtcttcat ccagcggatc ctctgtggcg agcagatatt
     2461 caatgaacag ttcttcaccg gccccgatcg gccggagcgt gaggataaaa atgcgaccgc
     2521 cgtcttcgat agtctcgcag ttcggcgcac aggcgtgatt gagccagcgc gcgctattgc
     2581 caccacggct gccgtcgatc acccgcccat ccgacagccc gaaaagaaac gtatgtcctt
     2641 cgacgccttc acgttggtga cggcgcaccg catttcgcca tgaggtgatt tcacctctgt
     2701 actcaagcac gcgctcgccc gccgcgagcg catgcatggc gaacacgcct ttgccgtgaa
     2761 ctggagaccg ccgaacaatc acgcgccgca tactctttcc tttcccgatg tcagcgccag
     2821 tatacgagca gcaaatttcc gcatccagtg tgatgggatg agcacgcact tgccacgttc
     2881 gggcaccggc gggcatatcg aagctggaga gtcgaggtat ctgatccgca ggcatcccga
     2941 aacgaccgac gatcgcccgc gaagagatcc agcttcagaa aagctgatta cccagctcac
     3001 gcaggctgac ccacttcacc gtgctcacga aggtcctgcc tgtggtatcg agcgattcga
     3061 tcatcgcgcg ttgcccgcgg gcttccgcaa tgacgcgcac gatgcgcgtc cggtaacgca
     3121 gtaacgcgcc tatcgttggg cacgagattt cgcgcccggt ccgcgatcgt ttcttttcca
     3181 tacgggtatc tatagagaca tgtagcgaat cgcaggggcc gcagtacgtc gcggcatctc
     3241 gcactgatgc taccaggccg actggccggg gtccaggcct tgcccgatca gtccgtgccg
     3301 ttgctgccgc cagcacctgt agtagccgct accctgccaa ctgccgtgcc atcggcagaa
     3361 atccggccga cggcttgcgt ctggtctcag gcgccgttca cggcgtcctt gaacgccttg
     3421 cccgccgtga acttcaccgt cttcgcagca gggatctgga tctcggcgcc ggtcgccgga
     3481 ttgcggccga cccgcgcggc gcgcgcgccc gtcgaaaacg agccgaaacc gatcagctgt
     3541 accgttttgc ccttcgtcag ttcacccgtg atggcggcga tcaccgcgtc gatcatctcg
     3601 cccgtggcga ccttgctgtc gccggtcttc gcggcgaccg catccaccag ctcctgcttg
     3661 ttcattgtga ttcctcgttg tggttgattg atgttgccac cctacccgat cggtgccgct
     3721 ttgggcaatc ggcaccccgg agcacgggtt catccgctcc agatgagtca cccgatgctg
     3781 gccgatcatc ggtgtcgcgt ccactgtccg gccgttgata ccggctgggc gaccttggat
     3841 gtgcaaagac tgaagcacct gcaaagagat ccatggttct gtaccgaagc cccttcgatc
     3901 ctccgccgtc tcggaccgaa gcaggccatc gacattccga cacacgtggt gggccttccg
     3961 cgtctgctgc tgcgcggccc cgccgtgccg cgtgacctcc ggtcaaatca cggcagtccg
     4021 gtcgcgcgcc ttcaggctcc ttcgcctaag gccgggtccg gttatgcgcc atcgcctgtc
     4081 agtcatcagg gggagagacg cccgtcgccc tcctcccggc tgaaaccgac caaaacccct
     4141 cctgcacgcc agtgtccgca aatgaagggg tagatagccg caactatcca ccccttcagt
     4201 tttttcacga aaacaggtac atttttccct gagcctgtcc gaaggcccgc cgcgcaaggc
     4261 gctcgggtcc gtcaccacaa aaagcgggtg cacgggccac ccgtccgtcg acatgcagcc
     4321 ggcgcagtcg cccagcgtgc gaggaaggga aagttgcttc gttccggaag taaaactagc
     4381 agcagtgcag gcttaaactt ggtgacggaa cgggaacgct ccgcgcccca atagggcttg
     4441 ttgctcgatc gaccagcgcg ggctgttaag aatgctttta atacacggaa tgccgcgctt
     4501 cggctggcat acaaaagtct tgtcagcagt gtcggtcgcc cccataatac tgagcgagtg
     4561 gactgggtgt tcccggtcgg cgtcaaagcc tcggtgagtc tcgccggggc ttttcgcttt
     4621 ctcgcgacgg gaaaatcttc agtccccggt cctcgtagcc ttctcccaga ccttcgcggc
     4681 cgccgctgca atatcctgtg cagagcatta gcgcttaccg tgttcatacc cttagtcgag
     4741 catcacccgt ttctaaggac agttactatc ctcactggtt tgcaaagctt gtcaaagcga
     4801 tgacgaaata ggttgaacct gcatcctgaa gcctgttttt cgtgccggct gccacttgtg
     4861 gcgagccgcg aatttgccaa ggacggggtg cgcggatctc atcgaggctg cgggcgtgct
     4921 tcggcaactg cccacctggc aagccggata catgtatgca gaccgtttcg acatcacgaa
     4981 caggaagctt gcaaaaggcg tcgggtccat acaagcacga aaaggtcgaa taccccgtaa
     5041 ccgaacattg caccgcgcgg ccgcgaacct tcactataaa gacgcagccg ctcacccacg
     5101 cacaccgtga tcaagcgagc cagtcaggtg catgaggcaa ggttgcattc tggacaacgt
     5161 cgtgattgac aaacaaggtc gcgccctcag tctccaaaag ctcttcgacc ctgttcatcg
     5221 acgcaattga ctgcactgcg tcgacattga aagaaggaac gcatctgcac tgaaagttgc
     5281 gccgattgtg agccacatcg ccacttaaca gcacgcgccc agcctcggga agattgagca
     5341 acagcgagca atggccaggg gtatggccgg gcgtgaaaat gatacggacg gcgccatctc
     5401 cataaacgtc gtggtcgccc tcgatcagcc tgagcctgtt gttacgcagc gtgccgtaca
     5461 gttcaggcct gaatccgaac gcgtcaggat ccgccccaaa catcgcctcg tattcagcct
     5521 tctgcacgat ccactcggcc ttcgtgaaca gccggcaatt gcccacatgg tcgtaatgcg
     5581 catgcgaaag cgccagcgta ccgatttcat caggctcaac cccgatttcc tgaagttgcg
     5641 acgcgatcgt cttactgatc gttccgcgga taccgtgggc gatgatccgg ccttccggct
     5701 cgccgatcag atcgtcctgg gtgccggtgt cccatagcat ccattgtccg cgatggcgga
     5761 tgagatacgc gttgcagctc aacgtcatcg gtttgccgac attgaatccg ggcgagtaaa
     5821 tcgatgcatc atcgacctgc gctattccag cgtcgagaac gtagagtcgc tcgattgtcg
     5881 cgtcatggct catggctgcc cccgttctca gcgaagatga atggcgagct caccgagccc
     5941 ttcgatcgca gacaccactt tgtcgcccgc tttaagccat tgacgctcgt cacgcggtac
     6001 cttttgcccc cagatcaccc ccggcggcgt cccagtaaaa aggatgtcgc ccggctcgat
     6061 cgtcatgatg ctggccacgt agcggatgat ctcgcggcag tcgaagatca tttcgccggt
     6121 gttgctgtcc tgcctgattt cgccgttcac ccaggtttgc agcctcaggt tgttcggctc
     6181 gggcacacga tcgcgggtag cgagccaggg acccaatgga gcaaaaccat ccgacatctt
     6241 ccccgccgtg aattgcgtcg tgatgttctg caaacctcgc gcgctgaagt cattgccgac
     6301 cgcgtagcct gcaacaacgg attgcgcatt ctccttcgac acgttcttgc atttccgccc
     6361 gaaaacgatc acgagttccg tttcgtagtc gaattccttg tctaccatag caggcagctc
     6421 aatgtcgccc tcatggtggt tgagtgcgtt cgcaaacttg ctgaagatcg gcggcgcctt
     6481 gggaataggc gttcccgtct ccgccgcatg cgcctggtag ttgaacccca cgcagatgat
     6541 cttttgcggc cgggtaacaa gtggggcgaa agcggcctca cgtacctcga ccgaatcgat
     6601 accgccgtcc tgccgcaggc ggttgacgat ggccgacacg tcggcagcac gaccgttctg
     6661 cagcaaatcg tccatatcca gcggcgcagg catttcgaac ttggcgccag cctgcgtaat
     6721 attcacgatc cggccgtcga gctcggcgcc gagtgcgagt gcaccatcca tgcggagatt
     6781 ggccaacttc aagccgagct cccctacttc aagattcggg tttacgtttt tcaatttgca
     6841 ctccttatgg aaattgcgaa tacggaatga agaaatgggg cagcctcggc gatcgcagga
     6901 acgagacgcc gcgaggttga cctccgaagc gtccgccaaa gtctgctggt agaatatatc
     6961 tacgatagga gaatgtcaac ggccgccctt tttggcggca acagcttgcc cggcttactt
     7021 atcgtcgcgc cgggagataa ccaggtaata ggagcaatgc tttctggagc gattactgaa
     7081 agcgtgctcg accctggcct cgaagaacag ggtgtcgcct tccgacaagc ggatttcctg
     7141 gtccggcagc gaaacgatca actccccttc tgtcacggtc acgtgctttt ctgttccggt
     7201 tcggtaggcc ggcatcatgc cagtcgacac gccagccggc agccagtgct ggagcaactc
     7261 aacctggctg cccaccatgg tcggggaaag aatgtggcgc tcgaagccgg tttcgctgtc
     7321 gcgaaaaata tgccgctgct cgcggcgaac caccactacg gcttgccggt cctcgacgcc
     7381 acctgtaaga aagctcagcg tcgtctcgag agcgtgcgcg atccgttttg ctactccgat
     7441 ggtcggactc ttttcgcctc gctcgacctt cgaaagcatc gcccggctca cgccggacct
     7501 cgtcgcgagt tcttcgagcg tcagctcgcc gcgctcccgc aacgcgcgca tgcggcgccc
     7561 aaattcctga ttcaactcgt cgtggtcgcc tgcagcttca ggtggaagtt ccataagact
     7621 gttcatctat gactgttcat aaggggtcgg cgaaagtcta gcgcatcgtc acacgctttt
     7681 ttgaaaacgc ttgaaggctc gctttctgtc tgctagtgta tcttccatag tagattgtgt
     7741 ctaccatgta agacgattca atcggcttct gaagaaatgc acagcgcagt gcaataaaac
     7801 ccttgttatc cgggctagcg cctcgccgcc ttggccatgg ctcaaacccg aatcaggaga
     7861 caacatgact caagcaatcg acgcactgtt cgagcaggac cgtgcgcatt tcatgcatcc
     7921 gtccacgcac gctcacgatc accagagcgg cgcactgcca ggcaggatcg tgcgcggcgg
     7981 cagcggcatc cacatcgaag atcatgaagg ccggcggtac atcgacgcct ttgcgggact
     8041 ttactgcgtg aacattggct atggccgtac cgaagtcgcc gacgccatgt atgagcaggc
     8101 aaagcagctt gcctattacc acacctacgt cggccattcg tcggacgtca tcatcgaact
     8161 gtcttcgcgc ctcatggaat gggcgccgcc tggcatgaag aaggtgtact acggcatgtc
     8221 cggctcggac gcgaacgaaa cgcagatcaa gatcgtctgg tactacaaca acgtgctggg
     8281 ccgtccgaac aaaaagaaga tcatctcgcg cgaacgcggc tatcacggct cgggcatcgt
     8341 gaccggcagt ctcaccggcc tgccgagctt ccatcagcat ttcgacctgc cgatcgagcg
     8401 cgtgaagcac acggtctgcc cgcactggta tcgcaacgcg ccagttggaa tgaatgaggc
     8461 cgcgttcgta gcgtattgcg tggacgagct cgaaaagctg attgcacgtg aaggcgccga
     8521 cacgatcgcg gccttcatcg gcgagccggt gatgggcacc ggcgggatcg tgccgcctcc
     8581 agccggctac tggcccgcga tccagcaggt gctgaaaaag cacgacattc tgctgatctg
     8641 cgacgaagtc gtatgcggct tcggccggct cggttcgaag atgggtgcgc agcactttgg
     8701 catccagccc gatctcatta ccgtcgcgaa gggactcacg agcgcttatg cgccgctctc
     8761 cggtgtgatc gtgggcgaaa aggtctggga cgtgattgcg cggggctcgc aggagcacgg
     8821 accgatggga catggctgga cctattccgg ccacccggtg tgcgcggccg ccgcgctcgc
     8881 caatctcacg atcatcgagc gcgaaaagct ggtggaaaac gccgcgcaag tgggctcgta
     8941 cttcggcgcg caactgcgtg aagcgttcgg ctcacacccg ctggtaggcg aggtgcgcag
     9001 cacgggcatg ctggctgccc tggaattcat ggctgacaag gaggcgcgca agcccttcga
     9061 cccggccctg aagatcgggc cgcgggttgc cgccgccgcg ctgcagcgag gtctgatcgc
     9121 gcgcgccatg ccgcacggcg acatcctcgg cttcgcaccg ccgctcgtga tgcgccgcga
     9181 cgaggcagac gagatcgttg gcatcgtgaa gcaggccgtg gacgaggttg cggagcgggc
     9241 tctcgcagac gcgggctgac cttgccgctt cgctccgcat cctgttggta attgtcgcgt
     9301 tgaacggagg cacctgtgcc gaagactcaa gaagtgattg tcagctttcg aggcgttagg
     9361 aaaacttacg atggcgagtc gctcgtcgtc aaatcgctcg atctcgatat ctaccgcgga
     9421 gaatttctca ccatgctcgg accatcaggc tccggcaaga cgacctgcct gatgatgctt
     9481 gcaggcttcg aatttcctac cggtggcgag atccacctcg acggcaagct gctcaacaac
     9541 gtcccccccc acaagcggga cattggcatg gtgtttcaga actatgcgtt gtttccgcac
     9601 ctgacggtcg gacagaacgt ggcgtatccc ctcagcgtgc gcaaggtgcc tgctaccgaa
     9661 cgtgccgcgc gtgtgaacga agccctgagg atggtacgca tggagagttt cgagaagcgc
     9721 tatccggcac aactgtcggg tggccagcag cagcgcatcg cgctcgcgcg cgcactcgtg
     9781 tccaaaccca agctcgtgct catggacgag ccgcttggcg cgctcgacaa gcaactgcgc
     9841 gaacacatgc agtacgagct gaagtccctg cacgcagaac tcgggctgac gttcgtctac
     9901 gtcacgcacg atcaggacga ggcgctcacc atgtcggacc gggtcgcggt gttcgacaag
     9961 ggcgtcgtgc aacagctcga cacagtcgac cgtatgtacg agtcgccatg caatgagttc
    10021 gtggcgaact tcatcggcga cagcaaccgc ttgcgcggca cgattgcgtc agtggacggc
    10081 gatttctgcg acttccggct cgacgacggg acgcggctcg tcggccgcaa tatcggcaag
    10141 gcggcggcgg gcgcctccgg catcgcatgc atccgccccg agcgcatgaa cgtggtgtac
    10201 ggcagcgatc gagcgaacga cgcagcggca aacgcgctgg cgggagaggc gcgcagcctg
    10261 atctattttg gcgaccacgt gcggatgcgc tgtgcgttca aggggcagga cgaatgtttc
    10321 gtcaaggtgc cgatcggcac cggtgcgctt gaacgattcg ctcccggcgc acccgtgacc
    10381 ctcgcattcg cgcctgaaca tttgcgcgtt ttcgcgtagc tgtctacgtg atttccacgc
    10441 tggccagtcc gacacaacga ttcgttttcg tcatgaggag aaggaaccat gatcactcga
    10501 gcaaagcttg ccgcgccctg cacggtgctc gcgctcacgc tggtgatgtc cggagcaccc
    10561 gcatccgctg cggaactgac ggttgtcaac ttcggcggta gctgggggca agtgcagaac
    10621 atcgccttca accagccgtt cgagaaagcg accggcaaca aggtcaccgt cgtcgagtac
    10681 aacggcgagc aggccagggt aaaagccatg gtcgaggcaa agcacgttaa ctgggacgtc
    10741 gtcgaggtcg agtcgggcga gctgaaccgc ggctgcgatg agggtttgta tgaacagctc
    10801 gactggtcga agatcgcgaa gaagtcggat ctggtccctg aagcgccgca aacctgcggg
    10861 gtcggcttcg ttgtgtggtc gatggcgctt gcctataacg ccgacaagct caagacggcg
    10921 ccgacgagct gggccgattt ctgggacgtc aagaaattcc ccggcaaacg cgcgatgcgc
    10981 aaaggtgcgc gctacaacct cgagttcgcg ctgatggccg acggcgttgc gccgaaggag
    11041 gtgtacaagg tccttgccac aaagaccggc caggatcgcg cgttcaaaaa gctcggcgag
    11101 ctgaaaccct acatccagtg gtgggaggcg ggtgcacagc cgccgcagtt tctcgtcgcc
    11161 ggtgatgtcg tgatgtcgac ctcgttcaac ggccgcatcg acgctgcaca aagagaaggc
    11221 cagaatctga aggtcatgtg gaacggcagc atatacgacc tcgactactg ggtgattccg
    11281 aagggcacgc cgaacaaggc gctcgccgag cagtacatcg cgtacacgct cacgccaaaa
    11341 ccgcaggtgg cgttcacgca gcatatcgcg tacggcccgg cagatgtcgt ggcgctcaag
    11401 tcgctcgacg ccaagaccct cgcgaatctg cccaactcgc cgacgaacgc caaagacgcg
    11461 accctgcaga acatcggctt ctggaccgat cacggcgacg aactcgagca gcgcttcgcc
    11521 gcatgggcct cgaagtgagc gtacggctgt ttttctgacc caaccggccg cgcggcgcgc
    11581 atcgcgttgt gcggcattcc gccggatcgc cgtccggccg gagaaaaacg ttgaatacga
    11641 tgacgatcgc cccctcctcg tcgacactgt cgacggccgc gctcaaacgc gaactccgct
    11701 ccgccggggc tcgcaaacgg gcgatggcgc tcctcctgat cgcgccgctc gcgattttcg
    11761 tcctgctcat cttcgtcgtg ccgatcggcg cgctgctcac acgtgcggtg cagaaccccg
    11821 agatcgcgac ggcgctgccg aacacggtcg cgacgttgtc gggctgggac cgtcacgcgc
    11881 caccgccgga cgccgcctac gcggcgctcg cagccgacat gacgaaactc gccggcagcg
    11941 acgaaatggg cacgctcgcg tcgcgtctga acctggaaat tcccggcttt cgttcattcg
    12001 tggcgaaaac cgtgcgcgcg atgccgctca agggcagcag cgaccagccg ctgacgcccg
    12061 cgcagacgcg cgagaagctg atcgagttcg atgcgcgctg gggcgacgcc acctactggc
    12121 aggcgatcgc aaaaaacggc agccagatct cgccgttcta tctgctcgcc gcgctcgacc
    12181 acaggcagga cggcttcagc catatcgtgc acgccgatcc cgagcagtcg atctacctca
    12241 cgatcttcgg ccgcacgttc ctgatcggta tggcggtcac cctgttcgcg ctgctgctag
    12301 gttatccgct cgcgtactgg gtctcg
//
LOCUS       DATASH010000369         2775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3613670,
            whole genome shotgun sequence.
ACCESSION   DATASH010000369 DATASH010000000
VERSION     DATASH010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3613670"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..1081)
                     /locus_tag="VHV99_10340"
     CDS             complement(77..1081)
                     /locus_tag="VHV99_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014252693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX3635871.1"
                     /translation="MELFQLSRANDVQDAIKAGAASHTAQQGADVRFLAGGTTLLDLM
                     KLNVERPGRVIDISRLPLNGVEALPDGGVKIGATVRNADLAHHALIRDAYPVLSQALL
                     AGASAQLRNMATTGGNLLQRTRCVYFRDTAMACNKREPGSGCAAISGFNRTLAILGVS
                     DACIASNPSDMNVALTALEAVVHIQGTRGERSVAIDDFFLLPGTTPERETVLEPGDLI
                     THVTLPPPAEGSRSLYLKLRDRASYEFALASAAVVVTVTDGRVSHARIALGGVGTKPW
                     RSREAEAALASATPDAASFRRAAADALAGARPQSDNGFKVELAQRCLVHALTLATSAA
                     "
     gene            complement(1093..1656)
                     /locus_tag="VHV99_10345"
     CDS             complement(1093..1656)
                     /locus_tag="VHV99_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX3635872.1"
                     /translation="MHDQSSPTRFIDETVGPKAGAPAPHARTQSTEPVTIAVELSING
                     RNYALMLDPRTTLLDALREHLHLTGTKKGCDHGQCGACTVHVNGRRVNACLCLAANHA
                     GDEITTIEGIGQSSALHPMQASFVECDGYQCGYCTSGQIMSALALLEEPVGPSDADVR
                     EAMSGNLCRCGAYQNIVAAIQVVRGKA"
     gene            complement(1874..2065)
                     /locus_tag="VHV99_10350"
     CDS             complement(1874..2065)
                     /locus_tag="VHV99_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HEX3635873.1"
                     /translation="MRLLLAIFLPWLQFFTIGRPLAGIICLLLQITVIGWIPAAIWSV
                     YALSEYKTDRKIARAVAGR"
     gene            complement(2271..>2775)
                     /locus_tag="VHV99_10355"
     CDS             complement(2271..>2775)
                     /locus_tag="VHV99_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011694769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium-independent anion transporter"
                     /protein_id="HEX3635874.1"
                     /translation="DGILLAVALSLLRHVRHSYHPHTMMLAPTPATGGRWAPVPAAPG
                     TETAPGLIVYRFGADLFFANDHFFVDDVRRLLDRAPTPVDWFIVDAGAITDLDYSAAR
                     TLAEFCEGLKQRGVNVLFARANQYLRADMDRHGITAVLGESHIFETLHQALVAAGFDP
                     SAASSTH"
BASE COUNT          530 a          848 c          883 g          514 t
ORIGIN      
        1 cgcgagtgcg gccggccgat gaccgcatgc agggaatcga gcacagtaga catggggtgg
       61 gttcctttcg acgagatcag gcggcgctgg ttgcgagcgt cagtgcgtgg accaggcagc
      121 gttgcgccag ttcgaccttg aagccgttgt cgctttgcgg ccgggcgccg gcgagtgcgt
      181 ccgccgcggc acgcctgaag cttgccgcat ccggtgtcgc ggaggcgagc gcggcctcgg
      241 cctcacgtga gcgccacggc ttcgtgccca cgccgcctaa cgcgatgcgt gcatggctga
      301 cgcggccatc ggtcaccgtg acaaccaccg cagccgacgc cagcgcgaat tcgtacgagg
      361 cccggtcgcg cagcttcaga tagagggagc ggctgccttc tgccggcggt ggcaacgtga
      421 catgcgtgat gagatcgccg ggttcaagca ccgtttcccg ttcaggcgtg gtgccgggca
      481 gcagaaaaaa atcgtcgata gcgacgctgc gctcgccccg cgttccctgg atatgaacca
      541 ccgcttcgag cgcggtgagc gccacattca tgtccgatgg attactcgcg atgcacgcat
      601 cgcttacacc gaggatcgcc agggtccggt tgaaaccgct gatcgccgcg caccccgagc
      661 cgggttcccg cttgttgcag gccatggcgg tatcgcggaa atagacgcac cttgtgcgtt
      721 gcaacagatt gccgcccgtc gtcgccatgt tgcgcagttg cgccgacgct ccggcaagta
      781 acgcctgcga cagcaccgga taagcgtcac gaatcaacgc atggtgcgcg agatcggcat
      841 tgcgtacggt ggcgccaatc ttcacgccac cgtccggcag cgcctcgacg ccattcagcg
      901 gcaggcggct gatatcgatc acgcggcccg gccgctcgac gttcagtttc atcaggtcga
      961 gcagcgtcgt gccgccggcc aggaagcgta cgtcggcgcc ctgctgtgcg gtatgagacg
     1021 ccgcaccggc cttgatcgcg tcctgcacgt cgttcgcgcg ggaaagctgg aacagttcca
     1081 tgtcggcgct cctcaggctt tgccgcgcac gacctggatc gcggcaacaa tgttctgata
     1141 ggcgccgcag cggcacaggt tgccgctcat ggcttcgcga acgtcggcgt ccgacggtcc
     1201 aaccggctct tcgagcaacg cgagcgcgga catgatctgc ccggacgtac agtagccgca
     1261 ttgataacca tcgcattcca caaacgacgc ctgcatggga tgcaacgcgc tggattggcc
     1321 gatgccttcg attgtcgtga tctcgtcgcc cgcatgattg gcggcgagac acaggcaggc
     1381 attcacgcgg cgtccgttca catggacggt gcaggcgccg cactggccgt ggtcgcagcc
     1441 tttcttcgtg ccggttaaat gcaggtgctc acgcagcgcg tccagcagcg tggtgcgagg
     1501 gtccagcatg agcgcgtaat ttctgccgtt gatcgacagt tcgacggcaa tagtgacagg
     1561 ctcggtcgac tgcgtgcgcg catggggggc tggcgcgccg gctttcgggc cgacggtttc
     1621 gtcgatgaaa cgagtggggg atgactgatc gtgcacggcg gaaatctcct gactgtgggg
     1681 acagggagaa gtcggtcacg tcgcagatac gctcttcagg atgccgatac gtctggcggg
     1741 tgtcgcgtcg ctgaccaaca acgtggctag taacaacgcg actagtatag gcagtcattg
     1801 cggagtttgc tgaacgccgg tttcgaccac gcgtgcagct tatgacgatc agtccgtcac
     1861 gccccttctc cgcctaccgt ccggcgaccg ctcgtgcgat tttccggtcg gtcttgtatt
     1921 cgctaagcgc gtagaccgac cagattgccg ccgggatcca gccaataacc gtgatctgca
     1981 gcagcaggca aatgatcccg gctaacggcc ggccgatcgt gaagaactgg agccagggca
     2041 agaaaatggc gagcaggagg cgcatggagt tttccgtcgt gttagaagaa ggccgtccga
     2101 aatcggcgac ccgttatttc gtcgtgcaag gacgtgataa cggtccgcta gtccaaaaac
     2161 tttagccctt tcttcgtgac atcgaaaaca cgctgctctt ggaacttatg ccgcatcccg
     2221 attcggatca acgactgcct gcgactccat gatgcatctt caatgcatat tcagtgcgta
     2281 ctcgacgctg cagatggatc gaatccagcc gctaccagcg cctgatgcag cgtctcgaag
     2341 atgtgcgatt ctcccagcac cgcggtgatt ccgtgacggt ccatatcggc tcgcagatac
     2401 tggttggcgc gcgcaaacag cacgttgacg ccgcgttgct tcagaccttc gcagaattca
     2461 gcgagggtcc gggccgccga atagtcgagg tccgtgatag cgccggcgtc aacgatgaac
     2521 cagtccacag gcgtgggcgc gcggtcgagc agacggcgta cgtcatcgac gaaaaaatga
     2581 tcgttcgcga aaaacagatc agcgccaaag cgataaacaa tcagcccggg agcggtttcg
     2641 gtacctgggg cggcaggcac gggcgcccag cgtcctccag ttgccggagt gggcgcaagc
     2701 atcatggtgt gcggatgata gctatggcgc acatgacgca gcaacgacaa cgcgaccgcc
     2761 agcagtatcc cgtcc
//
LOCUS       DATASH010000370         5591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4009969,
            whole genome shotgun sequence.
ACCESSION   DATASH010000370 DATASH010000000
VERSION     DATASH010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5591
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4009969"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(239..940)
                     /locus_tag="VHV99_10360"
     CDS             complement(239..940)
                     /locus_tag="VHV99_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX3635875.1"
                     /translation="MPDLPAHLWYSITSLGGAGMTLPLAFAIALWLAVGYTWRMAAGW
                     LLLLGAAIGVVTVTKLAFLGWGVGVRELDFTGVSGHAMLSTAVYPVAMFLMLLPARPA
                     IRLLGVLLGIAAGIAVGLSRVVLSAHSPSEAVTGCLVGALAALVFVRMAWNAEPGRLS
                     ALPVTASLMILAVLMHGVHVPTQRWVTHIALKVSGHDKPFIRAKWKAVRDVRPATAPL
                     SQTLNTLAPPHSSDI"
     gene            1320..2516
                     /locus_tag="VHV99_10365"
     CDS             1320..2516
                     /locus_tag="VHV99_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEX3635876.1"
                     /translation="MAKSLSKIWLSGLKRLLAIQTEHTHATTKRASKRPVRAATSKPS
                     AKARPVKPAAALRASAKREAPRTAPRESRVRPRASAWASGSWTRSFHSAPAAPGRLVN
                     HLQYGLYIPSGHALEAMPLVVMLHGCTQSIDEFAEGTRMNVLADRFGFAVVYPEQSKH
                     VHSHRCWHWYDASDSAGGAEARAVVSLVDSLVAQHGFDSERVYVAGISAGAGLTALLA
                     VNYPEHFAAVALHSGPAFGEARSGIAAMDVMRRGARREPAELVDELVNVAEYPGMPAI
                     ILHGDADHVVAPVNADQLAVEFLRLNRIVDEKGARKSGEVREERKSGVMMRDYIRGGR
                     RVVRLCRVQGLAHAWSGGDDAVPFHSAKGPDASALVWEFFRHQRRINAGRIAESAVTE
                     ASAGAR"
     gene            2624..2800
                     /locus_tag="VHV99_10370"
     CDS             2624..2800
                     /locus_tag="VHV99_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3563 family protein"
                     /protein_id="HEX3635877.1"
                     /translation="MYLLSRLFLFLTKSPDQLAKERADAYLAEATDLYDLEFRMRKLD
                     REASTRRPSWMSQH"
     gene            complement(3108..4607)
                     /locus_tag="VHV99_10375"
     CDS             complement(3108..4607)
                     /locus_tag="VHV99_10375"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEX3635878.1"
                     /translation="MTRARRLNDTFDTGLVWFRRDLRNTDNAALYYALKHCERVWCLF
                     VFDTTILQPLIGAWQARHPDTQPQDRRIEFILAALSELDAALRAKGGGLIVLYGDPAD
                     LVPRLAGELGVDAVFGNHDYEPVAIERDETVRERLAGAGRQLLTFKDQVIFEREEVLT
                     GQNKPFTVFTPYKNAWLKQLTAFDLKPYPVQTYAKHLVTLPASLDHPLPTLTQLGFAP
                     SNLAELDLPTGMSGAQRLLDDFLTRIDSYKDRRDFPAAKGPSYLSVHLRFGTVSIRTL
                     ARLAHEMSLQPDGEGSATWLSELVWRDFYFMILAHHPRLAGGASFKEEYDQLRWAQGP
                     EAGEAFAAWCDGRTGYPLVDAAMLQLNRTGYMHNRLRMVTASFLVKDLGVDWRLGERY
                     FAELLNDFDFSANNGGWQWAASTGCDAQPYFRIFNPITQSEKFDAEGRFIKRYLPQLA
                     KLPPKWIHAPWLAGAERLAEFGVILGKDYPAPIVDHAEARQRTLARFGK"
     gene            complement(4894..5313)
                     /locus_tag="VHV99_10380"
     CDS             complement(4894..5313)
                     /locus_tag="VHV99_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEX3635879.1"
                     /translation="MNILYKASATSTGGRDGRAVSSDNALDVKLAAPRELGGTGAAGT
                     NPEQLFAAGYSACFLSAMKFVAGQRKQTLPADTQVTAEVGIGPNDNGGFALDIDLRIS
                     LPGLAADAAKELVDAAHQVCPYSNATRNNVAVRLQVA"
     gene            complement(5488..>5591)
                     /locus_tag="VHV99_10385"
     CDS             complement(5488..>5591)
                     /locus_tag="VHV99_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX3635880.1"
                     /translation="EIFCATQQTPEFLLRLRDDLTRLRATLNDYMDH"
BASE COUNT         1073 a         1676 c         1780 g         1062 t
ORIGIN      
        1 cgctctgagg tgccagaggc cctgaggacg ctcaggttgt catgcgacgg gacctgtgag
       61 ggcaccggcg gctctgatga ttgtgcgggc ccggatggct ccgaaccgtc cgacggcgcg
      121 cgcttattat caaggtgatc gagctagccg ccgatcacgc cacgtcggcc caacaagacg
      181 agcaggtagt agccgagcac ggtgggcgat ccgatgagtg tggcagcacg agcgtgtttc
      241 agatatccga cgagtgtggc ggcgcaagcg tgttgagcgt ttgcgacagc ggcgctgtgg
      301 cgggacgtac gtcacgtacg gctttccact tggcgcgaat gaacggcttg tcatggccgg
      361 agaccttcaa cgcgatatgc gtgacccaac gttgcgtcgg cacgtgcacg ccgtgcatca
      421 gcaccgcgag gatcatcaga ctcgcagtga cgggcaacgc cgacaaccgg cccggttcgg
      481 cattccaggc catgcgcacg aagaccagcg ctgccagcgc gccgaccagg cagccggtga
      541 cggcttcaga cggcgagtga gcgctcagca cgacgcgcga caagccaacc gcgatgcctg
      601 cggcgatacc cagtaagacg ccaagcagac gaatcgccgg acgcgccggc agcaacatca
      661 ggaacatcgc gaccggatag acggccgtag acagcatggc gtgaccgctg acgccggtga
      721 aatccaactc gcgcacgccc acgccccaac cgaggaaggc cagcttggtc acggtcacaa
      781 cgccgatggc cgcgccaagc agcagcagcc agccggcagc catgcgccac gtgtagccga
      841 ccgccagcca aagtgcgatg gcgaacgcga gcggcagcgt cataccggca ccgccgaggc
      901 tggtgatcga gtaccacaga tgggcaggca aatcgggcat ggcaaaagcg cgaccaaggc
      961 cgcgtagaat cgggttggta atggtttaac gggtctaacg cgggtttgac ctgcaagcgg
     1021 catgaatcgc cgatgcagca aagaggcgtg taccgaaatg atttaccccg ccagtataga
     1081 cgggtgccaa gggccggact cgccacgcac caggcttttt gtgaccggaa gtgggctggc
     1141 gaacaagctt tgcaaaggtt tgacgttgat gttactgcaa ggttcaccga cggaaaattc
     1201 ggtgctcggt agcggctttg gccctcagca tcgcatgagg attggtgtta ttgtgcattg
     1261 cacaacaaag cgtcgttcga tagaacggca cctgtccctt tttgcgaggt tccccgccga
     1321 tggcaaaaag tctatcgaaa atctggctga gcggtctcaa gcggctgctc gccattcaaa
     1381 ccgagcacac tcacgcaacg accaagcgcg cctccaaacg gccggtccgc gctgccacca
     1441 gcaagccttc ggccaaggcc cgtccggtca agccggcggc agcgttgcgc gcatccgcga
     1501 aacgcgaggc gccgcgcact gccccccggg aatcgcgagt tcgaccacgt gcctcggcat
     1561 gggcgagcgg ttcgtggacg cgctcgttcc attcggcgcc ggccgcccca gggcggctcg
     1621 tcaatcatct gcaatacggt ttgtacattc cgtccggtca tgcgctcgag gcgatgccgc
     1681 tggtggtgat gctgcacggc tgcacccagt cgatcgacga attcgcggaa ggcacccgga
     1741 tgaacgtgct cgccgaccgg ttcggctttg ccgtggtcta tccggagcag tctaagcatg
     1801 tgcattcgca tcgctgctgg cattggtacg acgccagtga cagcgccggc ggcgcggagg
     1861 cgcgtgcggt cgtctcactg gtggattcgc tggtcgctca gcacggcttc gacagcgagc
     1921 gtgtgtatgt cgctggcatc tccgcgggcg ccggcctgac ggcgcttctg gccgtcaact
     1981 atccggagca tttcgcggcg gtcgcgctgc attccggccc cgcttttggc gaggctcgct
     2041 ccggcattgc ggcgatggat gtgatgcggc gcggcgcgcg ccgcgagccg gccgagctgg
     2101 tcgacgaact cgtcaatgtg gctgaatacc caggcatgcc cgccatcatc cttcacggcg
     2161 acgccgacca tgtggtcgcg ccggtcaatg cggaccagtt ggcggtggag tttctccggc
     2221 tgaaccgcat cgtcgacgag aaaggcgcgc gcaaatccgg cgaagttcgg gaagagcgta
     2281 agagcggtgt gatgatgcgc gactacatcc gcggcgggcg gcgcgtggtg cggctgtgcc
     2341 gtgtgcaagg gctcgcgcac gcctggagtg gtggcgacga tgccgtgccg tttcattccg
     2401 ccaaaggtcc ggacgcaagc gccctggtgt gggaattctt caggcatcaa cgccgtatca
     2461 atgcgggccg tatcgctgaa agtgccgtca cggaagcctc ggcgggcgct cggtgacgga
     2521 ggaacaacat ctcgaaatga acactggcgc gatctgaggg ttttccctat aatacgggtt
     2581 atcccttagg agttaacgcc taaattcatt gtcgaggttg accatgtatc tgcttagccg
     2641 cctgtttctc ttcctgacca aatcgcccga tcaactcgcg aaagaacgtg ctgacgctta
     2701 cctcgcagag gcaaccgatc tgtacgatct ggaattccgt atgcgcaagc ttgaccgtga
     2761 ggcgagcacc cgccggccgt cgtggatgag ccaacactaa gctgcccaag ttgaagcatc
     2821 ggcgcgtatt tgaagagcgt cggtgtgtgg gcgagttgcc caggttgtta caaattgcct
     2881 gaagcgctta ggcgcgctca gacaggctta tatagcggaa ttcaatcgcc accacgcgcg
     2941 attcaacacc gcggcgaacg ccatcaaagc catatggggc gcagtagttc gcccgcgcaa
     3001 agggtcccgt cgaccgaatg cacacgtcag cgcgacgaag caacgacgcc gccggcgcat
     3061 tttctagact ctttaacgtc ctcgatcaaa tttgcgtgag caggcgctca cttgccgaaa
     3121 cgggcaagcg tacgctgccg ggcttcggca tggtcgacga tcggcgcagg ataatcctta
     3181 cccaatatca cgccgaattc cgcgagccgc tccgcgcccg ccagccatgg cgcgtggatc
     3241 catttgggcg gcagcttcgc aagctggggc agatagcgct tgataaagcg cccttcagcg
     3301 tcgaattttt ctgattgcgt gatcgggttg aaaatccgga aatagggttg cgcatcgcat
     3361 cccgtcgacg ccgcccattg ccagccgccg ttattcgccg agaaatcgaa gtcgttcagt
     3421 aattcagcga agtaacgctc gccgagtcgc cagtccacgc ccagatcctt caccagaaag
     3481 ctcgccgtca ccattcgcag ccggttgtgc atgtagccgg tgcggttcag ttgcagcatg
     3541 gcggcgtcga ccaacggata gccggtgcgg ccgtcgcacc atgcggcgaa cgcttcgccg
     3601 gcttccgggc cttgcgccca gcgcagttgg tcgtactctt ccttgaacga cgcgccgccc
     3661 gccagcctcg gatgatgcgc gaggatcatg aagtagaaat cccgccagac cagctctgaa
     3721 agccatgtgg ccgagccttc cccgtctggc tgcagcgaca tctcgtgtgc gagccgcgcg
     3781 agtgtgcgga tcgaaacggt gccgaagcgc aaatgcaccg acagatagct cggacctttg
     3841 gcggcgggga agtcgcgccg gtccttatag ctgtcgatgc gagtcaggaa gtcgtcgagc
     3901 agacgctgcg cgccgctcat gccagtgggc aggtcgagct ccgcaagatt gctcggtgcg
     3961 aagccgagtt gagtgagcgt cggtaacggg tggtccaggc tggccggcaa ggtgaccaga
     4021 tgtttcgcgt aggtttggac gggatacggc ttcagatcga aggccgtcag ctgcttgagc
     4081 catgcgttct tgtagggcgt gaacaccgtg aacggtttgt tctgacccgt cagcacctcc
     4141 tcgcgctcga aaatgacctg gtctttgaac gtcagcaact ggcgtccggc accggccagc
     4201 cgctcccgca ccgtctcgtc acgctcgatc gcgaccggtt cgtaatcgtg attcccgaat
     4261 accgcgtcca cgccgagttc gccggcaagc ctcggcacga gatcagccgg atcgccgtac
     4321 agcacgatga gaccgccgcc tttggcgcgc aaggccgcgt ccaactcgct caacgcagca
     4381 agaatgaact cgatgcggcg gtcctgcggt tgcgtatccg gatggcgagc ttgccaggcg
     4441 ccgatcagcg gttgcaggat cgtggtgtcg aatacgaaga ggcaccagac gcgttcgcaa
     4501 tgtttgagcg cgtagtaaag cgcggcgttg tccgtgttgc gcaggtcgcg gcggaaccag
     4561 accagaccgg tatcgaaggt gtcgttcagg cgtcgggcgc gtgtcattgg aatcgtcggt
     4621 ctggtgaatg gttgtgcggc cgggcatcga gcggcgcatc gggttctgtc acgcgaaacg
     4681 tattgctgcg ctgcacggtt attcaccgcg tttagcgatg gtaagtgatc gcaccgtccg
     4741 gtattgcaca ttgcatgccg cgttcgccaa acggtacggc gcccgtacct gttttgcgag
     4801 tacgggcgcg ctttaaggtg acgcggtttc gccgcgccgc gccggttcaa ctactcacca
     4861 gcgattcaac accgatttca tcgaagccgc gacttacgcg acttgcaaac gcaccgcgac
     4921 gttgttgcgc gtggcgttcg agtacggaca gacctgatgc gcggcgtcga ccagttcttt
     4981 cgctgcgtca gcggcgaggc ccggcaacga aatgcgcaga tcgatgtcga gcgcgaagcc
     5041 gccgttgtcg ttcgggccga tacccacttc tgccgtgact tgcgtgtcag ccggcaaagt
     5101 ctgcttgcgc tggccggcga cgaacttcat cgcgctcagg aaacaggccg agtagccggc
     5161 tgcaaagagt tgctccggat tcgtgcccgc tgcgccggtg ccgcccagtt cacgaggtgc
     5221 tgccagcttc acatccaacg cattgtccga cgacactgca cggccgtcac ggccaccggt
     5281 gctggttgcg cttgctttgt agagaatgtt catggtgttg ctcctttgtt tgggttgtga
     5341 ggatttgcat gcctgccgct gtttgctcgg ccaccgttcc ggcggcgctc catcgaagcg
     5401 gcatgtgata aagatagtgc acaaatcatt tgtgtgcaaa tgaatttttg catcataccg
     5461 atagcgtgct tttttgaagc aggctggcta gtgatccatg taatcgttaa gggtggcgcg
     5521 cagacgcgtc agatcgtcgc gcagacggag caggaattcg ggtgtctgct gggttgcgca
     5581 gaagatttcc g
//
LOCUS       DATASH010000371         5644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2231221,
            whole genome shotgun sequence.
ACCESSION   DATASH010000371 DATASH010000000
VERSION     DATASH010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5644
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2231221"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VHV99_10390"
     CDS             <1..184
                     /locus_tag="VHV99_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3635881.1"
                     /translation="LGGFFAPAAFGYLQQHTGSITGGLYGLGVASLIAAAAGFLTRNR
                     RVTREAQPESLHREAH"
     gene            310..1659
                     /gene="gudD"
                     /locus_tag="VHV99_10395"
     CDS             310..1659
                     /gene="gudD"
                     /locus_tag="VHV99_10395"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HEX3635882.1"
                     /translation="MSTNTSQSNATPIVTELRVVPVAGRDSMLMNLSGAHGPFFTRNI
                     VILRDSAGHTGIGEVPGGESIRKTIDDARGLVVGQSIGNLQAILNKARTQFADRDAGG
                     RGLQTFDLRTTIHAVTALEAALLDLLGQHLGVPVAALLGEGQQRDEVEMLGYLFYVGD
                     RNKTDLPYASGAEGRDDWERVRTEEAMTPEAVVRLAEAAQARYGFNDFKLKGGVLPGD
                     AEIEAVTALAERFPNARVTLDPNGAWSLAEAVRLCRDQHDVLAYAEDPCGAENGYSGR
                     EVMAEFRRATGLPTATNMIATDWRQMGHAIQLQSVDIPLADPHFWTMQGSVRVAQMCH
                     EWGLTWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQDGQRLTREPLQIVGGK
                     VKVPQAAGLGIELDMDEIEKAHALYQQHGLGARDDGVAMQYLIPNWKFDNKRPCLVR"
     gene            1728..2645
                     /gene="kdgD"
                     /locus_tag="VHV99_10400"
     CDS             1728..2645
                     /gene="kdgD"
                     /locus_tag="VHV99_10400"
                     /EC_number="4.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010094246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047448 - 5-dehydro-4-deoxyglucarate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxyglucarate dehydratase"
                     /protein_id="HEX3635883.1"
                     /translation="MTSPQELKAIVSEGLLSFPVTDFDAQGDFRADTYAERLEWLAPY
                     GATALFAAGGTGEFFSLTKADYTNVIRTATETCKGKVPILAGAGGPTRVAIDYAQEAE
                     RLGASGVLLMPHYLTEASQEGIAAHVEQVCNAVKNIGVIVYNRANSKLNADMLERLAD
                     RCPNLIGFKDGVGEIEAMVTIRRRLGDRFSYLGGLPTAEIYAAAYKALGVPVYSSAVF
                     NFIPKTAMAFYRAIAADDHATTGKLIDEFFLPYLAIRNRRQGYAVSIVKAGAKLVGRD
                     AGPVRAPLTDLTEEETAQLDALIRKLGAQ"
     gene            complement(2662..4170)
                     /locus_tag="VHV99_10405"
     CDS             complement(2662..4170)
                     /locus_tag="VHV99_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3635884.1"
                     /translation="MLGIGLVNMLVALDQTVVSTALPSIVAELHDFEYYAWIASAYLL
                     ASVVTVPVFGRLGDYFGRKRFVIAAVITFTVASVLCGVANNMLFLVIARGLQGVGGGM
                     MVGTAFASIPDLFPDPRARVRWQVVMAAAYGIGTAAGPSLGGWMSEHWGWRSTFLINL
                     PVGAAALYFIWAHLPSFQRPHEGEVKIDWLGAALVAAVLGCLQAFIEAVPKNGLTAGN
                     LALAAFVIAGTIALLVCEKRATHPIIPLDLFKDAQLVTLFILGFLSGFVMFSLIFFAP
                     LLLQGGFGLSPQQAGLLATPIAACIALGSLLNTRIVLHMKKPTRILSIGFALLLFASI
                     ALAFANPETPHLWIELPMAAVGIGLGFILNNLNVFGQEIAGRERFGITTALLQSTRMV
                     GGMLGTSIVATVVQHHYRNVITRTMSVLGEPAISQWQPRFAELRILIDEASRLKLIAD
                     MKTSGLNTAALIDTARDALVQSIHIGVWLTAVAALAAALLVQRISHVVFRRS"
     gene            complement(4405..>5644)
                     /locus_tag="VHV99_10410"
     CDS             complement(4405..>5644)
                     /locus_tag="VHV99_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008342062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3635885.1"
                     /translation="SATLAFGLFVLASRGTARHQAFAARAASVDGELVDVIGNMGLVR
                     TFSAMDIEHQRLDGHLETEGGARTRSLLYLEKLRLLHSLATTVLSAGLLGWTLWLWTQ
                     DRATTGDVVLVGSLGFAILHGTRDLAVALVDLTQHVARLGEATQTLLIPHEMAERGNT
                     TPLQIQEATVDFDNVTFAYSGRRPVLSGFNLHIRAGERVGLIGPSGAGKSTIFALIQH
                     FYEPQSGCVRISGQDISQVTLQSLQAAISIVPQDVSLFHRSLLDNIRYGKPTATDEEV
                     RRACEDANCLDFLAAMPGGLKTIAGDRGTKLSGGQRQRIAIARAILRDAPILLLDEAT
                     SALDTASELAIQSALERLMKNRTVIAIAHRLSTLQTFDRIVVINRGRVVQDGAPAELA
                     GVPGIYRETLERQRKRVPTG"
BASE COUNT         1086 a         1782 c         1811 g          965 t
ORIGIN      
        1 cctcggggga ttcttcgcgc cggccgcgtt cggttatctg cagcaacaca cgggctcgat
       61 taccggcgga ctatacggct tgggcgtggc ttcgctgatc gcggcagcgg ccggcttcct
      121 gactcgcaac cgtcgcgtga ctcgcgaggc gcagccggag tctttgcatc gcgaagccca
      181 ctgacgggcg aaaaccgtcg accatgaatc acacctttcc cactttctgt ccggcatggg
      241 tctcgagcga gtttgtccaa cgcgcggatt cccacttgct ggctcactaa ctcaaccggg
      301 cctactctca tgtccacgaa cacatcccaa tcgaacgcga cgccgatcgt caccgaactg
      361 cgcgtcgtcc ccgttgctgg ccgtgacagc atgctgatga acctgagcgg cgcgcacggg
      421 ccgttcttca cccgcaacat cgtgattctg cgcgacagtg ccggccatac cggcatcggc
      481 gaagtgccgg gcggcgagag catccgcaag acgatcgacg acgcgcgtgg gctggttgtc
      541 ggtcaatcga tcggcaatct gcaagctatc ctgaacaaag cgcgtaccca gttcgccgac
      601 cgcgacgccg gtgggcgcgg cctgcagact ttcgatctgc gcaccacgat tcacgcggtg
      661 accgcgctcg aagccgcgct gctcgatctg ctcggccagc atctgggcgt gcccgtcgcg
      721 gcactgctcg gcgaaggcca gcagcgcgac gaagtggaaa tgctcggcta cctgttctat
      781 gtcggcgacc gcaacaaaac cgatctgccc tatgccagcg gcgccgaagg gcgcgacgac
      841 tgggagcgcg tgcgcacgga agaagcgatg acgcccgagg cggtggtgcg gctcgccgag
      901 gccgcgcaag cgcgttacgg cttcaacgat ttcaagctca agggcggcgt gttgccgggc
      961 gatgcggaaa tcgaagcagt gaccgcactc gccgaacgtt tccccaacgc ccgcgtcacg
     1021 ctggacccga acggcgcgtg gtcgcttgcc gaagcggttc gcctgtgccg cgaccagcac
     1081 gacgtgctgg cgtacgcgga agacccgtgc ggtgcggaga acggctattc gggccgcgag
     1141 gtgatggccg aattccgccg cgccaccggc ttgccgacgg cgaccaacat gatcgctacc
     1201 gactggcgcc agatgggtca tgcgattcaa ttgcaatccg tggatattcc gctggccgat
     1261 ccgcacttct ggaccatgca gggctcggtg cgtgtcgcgc agatgtgcca cgaatggggt
     1321 ctcacctggg gttcgcactc gaacaaccac ttcgatattt cgctcgcgat gttcacgcat
     1381 gtcgccgccg cggcgcccgg caagatcacg gcgatcgata cccactggat ctggcaggac
     1441 ggtcagcgcc tgacgcgcga accgctgcag atcgtcggcg gcaaggtcaa ggtgccgcag
     1501 gccgcgggtc tcggcatcga gctggatatg gatgagatcg agaaggcgca cgcgctgtat
     1561 cagcaacacg gcctcggtgc gcgcgatgac ggtgtggcca tgcagtacct gattccgaac
     1621 tggaagttcg ataacaagcg tccgtgtctc gtgcgctaag tctttaacga tctgaatggc
     1681 ggtccctgcg gccgcccgct tctacccaca attacgctat tcacgctatg acctcacccc
     1741 aagaactcaa ggcgattgtt tccgaaggcc tgctgtcctt tcccgtaacg gacttcgacg
     1801 cacaaggcga tttccgcgcc gacacgtacg ccgagcgcct cgaatggctc gcgccgtacg
     1861 gcgccaccgc gctgtttgcc gccggcggta cgggcgaatt tttctcgctg accaaagccg
     1921 actacacgaa cgtgattcgc acggccaccg agacctgcaa gggcaaggtg ccgattctcg
     1981 ccggtgcggg tggcccgacg cgcgtcgcga tcgactatgc gcaggaagcc gagcgtctcg
     2041 gcgcaagcgg cgtgctgctg atgccgcact atctgaccga ggcgtcgcag gaaggcattg
     2101 ccgcgcacgt cgagcaggtg tgcaacgcgg tgaagaacat tggggtgatc gtctataacc
     2161 gcgcgaactc gaaattgaac gcggacatgc tcgaacgtct cgcggaccgt tgcccgaacc
     2221 tgatcggctt caaggacggc gtgggtgaaa tcgaggcgat ggtgacgatt cgccggcgcc
     2281 tgggcgaccg tttctcctat ctcggcggct tgccgaccgc ggaaatctac gccgcggcgt
     2341 ataaggcact gggcgtaccg gtgtattcgt cggcggtgtt caatttcatt ccgaagacgg
     2401 cgatggcgtt ttaccgcgcg atcgccgccg atgatcacgc caccacgggc aagctcatcg
     2461 acgaattctt cctgccgtat ctggcgattc gcaaccgccg ccagggctac gcggtgagta
     2521 tcgtgaaggc gggcgcgaag ctggtaggcc gcgacgcggg tccggtgcgt gcgccgctga
     2581 ccgatctgac cgaggaagag acggcgcagc tcgacgcgct gatcaggaag ctcggcgcgc
     2641 agtaggcgac gcagaagggg ctcaactccg gcgaaacacc acatgcgaga tgcgctgcac
     2701 gagcaaggcg gcggcaagcg ccgccaccgc cgtcagccag acgccgatat gaatcgactg
     2761 caccagcgca tcgcgtgcgg tgtcgattag cgcagccgta ttgagccccg acgtcttcat
     2821 atccgcgatc agtttcagac gcgatgcttc gtcgatcaga atgcgcagtt cggcgaagcg
     2881 cggctgccat tgcgatatgg ccggttcgcc gagtacgctc atcgtgcggg tgatcacatt
     2941 gcggtaatgg tgctgcacca cggttgccac aatgctggtg ccgagcatcc cgcccaccat
     3001 tcgcgtggac tgcagcagcg ccgtcgtgat gccgaagcgc tcgcgcccgg caatctcctg
     3061 gccgaacaca ttcagattgt tcaggatgaa ccccaggccg atgccgactg ccgccatcgg
     3121 caattcgatc cacaggtgcg gtgtctccgg attggcgaac gccagggcga tcgaggcgaa
     3181 caacagcagc gcgaaaccga tcgagagaat ccgcgtgggc ttcttcatat gaaggacgat
     3241 gcgcgtattc agcaggctgc cgagcgcgat gcaggcggca atcggcgtgg cgagcagacc
     3301 ggcttgctgg ggcgacagcc cgaagccgcc ttgcaacaaa agcggcgcaa agaagatcag
     3361 cgagaacatc acgaagccgg acaggaagcc gagaatgaaa agcgtgacga gttgcgcgtc
     3421 tttgaacagg tcgagtggaa tgatcggatg cgtggcgcgt ttctcgcaca ccagcaacgc
     3481 gatggtgccc gcgatcacga aggccgccag cgccaggttg ccggccgtca gaccgttttt
     3541 cggcacggct tcgatgaaag cctgcaggca gcccagcacg gctgcaacca gcgcggcgcc
     3601 gagccagtcg atcttgacct cgccctcgtg cggacgctgg aagctcggca gatgcgccca
     3661 gatgaaatag agcgccgcgg cgccgaccgg caggttgatc aggaaggtgg agcgccagcc
     3721 ccagtgctcg ctcatccagc cgcccagcga gggacccgcc gcggtgccga tcccgtacgc
     3781 ggccgccatg accacttgcc accgcacccg cgcgcgcgga tcgggaaaga ggtcgggtat
     3841 cgacgcgaat gccgtgccca ccatcatccc gccgccgacc ccttgcaagc ctcgcgcgat
     3901 cacgaggaac agcatgttgt tcgcgacgcc gcaaagcact gacgcgaccg tgaacgtgat
     3961 gaccgcggcg atcacaaaac gcttgcggcc gaaatagtcg ccgagacggc cgaacacggg
     4021 gacggtcacc accgacgcca gcaggtacgc gctggcaatc catgcgtagt actcgaagtc
     4081 atgcagttcg gcgacgatcg aaggcagtgc ggtgctgacg acggtctggt cgagtgcgac
     4141 cagcatattg acgaggccga tgcccagcat agctagcaag gcgtttcgga aaggcagggc
     4201 agtcgcggtc gggttcacgt aaatggaagc aaagggcttt tcagggttgg cgctgcgcgg
     4261 acggggcgcg ggccagggtc ttgacgtcta ttgtcttaca gttgtctgac ctcttgccgc
     4321 cgattatacg ggttttccct taataaaaac tattcgaatc gggcaggaac ccgtactgcc
     4381 cgcttactgc gtcacatgct tttctcagcc agtaggtaca cgtttgcgct gccgctcgag
     4441 cgtctcgcga tagatgccgg gcacaccggc caactccgcc ggcgcgccgt cctgaaccac
     4501 gcggccccga ttgatgacga cgatgcgatc gaaggtctgc agggtggaaa gccggtgcgc
     4561 aatcgcgatg acggtgcgat ttttcatcaa ccgttcaagg gctgactgga tggcgagttc
     4621 ggacgcggtg tcgagcgccg aggttgcttc gtcgagcaac aggatcggcg catcccggag
     4681 aatggcgcgc gcaatggcga tgcgctgtcg ctgaccgccc gagagcttcg tgccgcggtc
     4741 gcccgctatc gtcttgaggc cgcccggcat ggcggcaagg aaatccaggc agtttgcgtc
     4801 ttcgcacgcg cggcgcactt cctcgtcggt cgctgtgggc ttgccatagc ggatgttatc
     4861 cagcaacgag cgatgaaaca gggagacgtc ctgaggcacg atcgaaatcg cggcctgcag
     4921 actttgcagc gtgacctgag agatgtcctg gccggaaatg cgcacgcagc ctgactgcgg
     4981 ttcgtagaag tgctggatca aggcgaaaat ggtcgacttg ccggcaccgg atggaccgat
     5041 caggccgacc cgctcgcccg cgcgaatgtg caggttgaat ccgctaagca ccggccggcg
     5101 tccagaatag gcgaacgtga cgttatcgaa gtccactgtc gcttcctgaa tttgcagcgg
     5161 cgtcgtgttg ccgcgctcgg ccatctcgtg cggtatcagc agcgtttgcg tggcctcgcc
     5221 gagacgggcg acgtgctggg tcagatcgac cagcgccacg gccagatcac gggtgccatg
     5281 cagaatcgca aagccgagcg agccgaccag caccacgtcg ccggtggtgg cgcgatcctg
     5341 agtccatagc cacagcgtcc agccaagcag cccggccgag agcaccgtcg tggcgagcga
     5401 gtgcagcagc cgtagttttt cgaggtacag caggctcctg gtgcgcgcgc cgccctccgt
     5461 ttccagatgc ccgtcgagcc gttgatgctc gatgtccatc gcggaaaacg tgcgcaccag
     5521 gcccatgttg ccaatgacat ccaccagttc gccgtccacc gatgcggcgc gcgcggcgaa
     5581 cgcctgatgc cgcgccgttc ccctgctcgc aagcacaaac agcccgaagg ccagcgtggc
     5641 ggaa
//
LOCUS       DATASH010000372         4037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_251870,
            whole genome shotgun sequence.
ACCESSION   DATASH010000372 DATASH010000000
VERSION     DATASH010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251870"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..1285)
                     /locus_tag="VHV99_10415"
     CDS             complement(167..1285)
                     /locus_tag="VHV99_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX3635886.1"
                     /translation="MVKSDQLIERLAAVFALIILVGGSLLVLAPFTTALLWGAILSYS
                     SWGLYRRLTAAVGGRRKLAATLIVLIILVVVLGPFVYAGFAFGAHVHDIVALVQKLFE
                     AGLPDLPPWVGRIPLVGSNIEAFWTNVTSSNSELIAQTRALAAPAGKWILAAAIAVTH
                     GLGLLALSIVLAFFFYTGGEGAAAWLRAGMRRIAGDRADYLLALAGSTVKGVVYGILG
                     TALVQGILAGFGCWIAGVPAPALLGLATFFLSVIPGGPVVVWLPAAIWLYHGGATGWA
                     IFLVVWGVLVVGMSDNVIKPILIGKSSDMPLILVMLGILGGAFAFGFLGVFIGPTLLA
                     VAYTVLHDWTIGAPAARALTPEVKVPAAEESGRIEKAP"
     gene            1552..2766
                     /locus_tag="VHV99_10420"
     CDS             1552..2766
                     /locus_tag="VHV99_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017331593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2863 family protein"
                     /protein_id="HEX3635887.1"
                     /translation="MRSRIAKRLPPDADKLVGLSLALFASGSRTEDRFWEAKLDALLA
                     KIVRNANQTTLDAALDHLQQNHPDAYGALADMAETHSESFILEHEGAPYEALLIAAPV
                     LAWTRYMIPSGPLKTDAADALRAHLQAHVLAANTRVAMVPFLYSIDQLPRHHVETWRI
                     AQQLAQAAMAGGNVKLNFGELPETSPILADPRFLLAVVAAPVGEPAFRWQEEENGSRI
                     ERGQCLEQWATQGGANLSLVLPGCEFECLLPDAYYSACRDADERVRPHTVRTAVRYLF
                     DTIGAAPQELRAVVAGFGERRIDEYRVGFTRRGSNDVIYGVVWPLYGRENGEPGIDEE
                     PQETAASEDPIEEIVALLKETGVTDVRRHSGRFEPEYCDDCGVPLYADPLGEIVHAEM
                     PEDAEPAQPHFH"
     gene            2979..3854
                     /locus_tag="VHV99_10425"
     CDS             2979..3854
                     /locus_tag="VHV99_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3635888.1"
                     /translation="MRFSILNSDAERRDGLKALLRQIDRLARFNEAQDWRQVERTLKR
                     LRPDLLVIDWQDWMSVDDARHLLSHYPDLRIAVLTDEVSPTVVRNLMDEGVLGVVPRE
                     TDPCLIVRAFEMVLLGGHYVPPGALALKPPLPPDAAIRPFDEKSPPPRRTKLSNGLSP
                     RQEQIMRCVHMGSTNKMIARTLGISEGTVKIHLTSIFQQLGAPNRAAAVALYNGWLSA
                     HLQVLRNGDAGPVRPVMGQAGVVPLRRRTLRRFQYPLPANDTADPLPMAAEPSTPYGG
                     VPAGPAAEQVTQGPA"
BASE COUNT          856 a         1352 c         1137 g          692 t
ORIGIN      
        1 tcgctacgtc gtcaacaggt ttttccaact ttctcaaccc gcccacttcg ctgaaagcct
       61 tgccactgct ggctctccag cctcccgtgc cccgttgtcc gaagcacgaa agagcgagat
      121 tctagcgagc cgaaccgcgc cttgcaagcg ttatctggaa tttatctcag ggcgcctttt
      181 ctatgcgccc tgactcctcg gcagccggca ccttcacctc gggcgtcaga gcacgcgctg
      241 ccggagcacc gatcgtccaa tcgtgcagca cggtataagc aactgccagc aaggttgggc
      301 cgatgaaaac gccaaggaag ccgaaagcaa acgcgccgcc caggataccc agcatcacca
      361 gtatcagtgg catgtcacta cttttgccaa tcaggatcgg cttgatgaca ttgtcggaca
      421 tgccaacaac caacacgccc cacacgacca gaaagatcgc ccaacccgtt gcgccgccgt
      481 gatacagcca gatcgccgcg ggcaaccaga cgacaactgg gccgcccggt atcaccgaca
      541 gaaagaacgt cgccaaccca agcagcgccg gagccgggac gcccgcaatc cagcaaccga
      601 agccggcaag aatgccttgc acaagcgccg tgccgagaat tccatagacc acgcccttca
      661 ccgtgctgcc cgccaacgcc agcaagtaat cggcgcgatc gccggcgatg cgccgcatgc
      721 ccgccctcaa ccacgccgca gcgccctccc ctccggtata aaagaagaat gcaagcacaa
      781 tactcagcgc caacaaaccc agaccatgcg taacggcaat cgcagccgcc aggatccact
      841 tgccggccgg cgcggcaagc gcacgcgttt gcgcgatcag ttcagaatta ctgctcgtca
      901 cattcgtcca gaacgcctca atgttcgagc cgaccagcgg gattcgcccc acccacggcg
      961 gcaaatccgg caagccagcc tcgaagagct tttgcaccaa cgcgacgatg tcgtgaacgt
     1021 gcgcaccgaa agcaaagccc gcatacacga acgggccaag caccacaacc agaatgatga
     1081 gtacgattaa cgtagcagcc agcttccgcc ggccgccgac ggccgcagtc aggcgccggt
     1141 acaagcccca tgagctataa ctgagaatgg cgccccatag caacgcggtc gtaaaaggtg
     1201 ccagcaccag cagggacccg cccaccaata tgatcaacgc gaacactgcg gcgagacgtt
     1261 caatcagttg gtcggatttc accatgaatc tcctgttatg ccagaccaca gcgaagaata
     1321 gacacgcttg ccagacgccc actgaagaca gtcagtatag gagcgagtcg cgcccttggg
     1381 cacgtcttat atcattcggc caaggcgcca tgcaacgcat ccggagccac tcgcaacagg
     1441 cttattttcg atacggaaac gtcccaatcc attcggcata ttcagccaaa taatatgtcg
     1501 gcatagaaaa gactagaata aaaggttctg tgcaccccaa cttccggatt tatgcgctcg
     1561 cgaatcgcca aacgtctccc ccccgatgcc gacaagcttg tcggtctctc gctcgcgctt
     1621 ttcgcgtcgg gcagccgcac cgaagaccgc ttctgggaag cgaagctcga tgcgctgctg
     1681 gccaagatcg tccgcaacgc caaccagact acgctggacg ccgcgctcga tcacctgcag
     1741 caaaatcatc ccgatgctta cggcgcactc gccgacatgg cggagacgca cagcgagtcg
     1801 ttcatcctcg aacatgaagg cgctccttat gaagcgctgc tgattgccgc gcccgtgctg
     1861 gcctggactc gctacatgat tccttccggc ccgctcaaaa cggacgcagc cgacgcgctg
     1921 cgcgcgcacc tgcaggcgca cgtgctcgca gccaatacgc gtgtcgccat ggtgccgttc
     1981 ctgtacagca tcgatcaact gccgcgccac cacgtcgaaa cctggcgcat cgcccagcaa
     2041 ctcgctcaag cagcgatggc cggcggcaac gtcaaactca attttggcga actgccggaa
     2101 acttcgccga tccttgccga cccgcgcttt ctgctggcgg tggtcgcggc gccggtaggc
     2161 gagccggcgt ttcgttggca ggaagaagaa aacggcagcc ggatcgagcg tggccaatgc
     2221 ctcgaacaat gggcaacgca aggcggcgcc aatctttcgc tggtactgcc cggatgtgaa
     2281 ttcgagtgtc tgctgccgga cgcctattat tcagcttgcc gcgatgccga cgaacgcgtg
     2341 cgccctcata cggtgcgtac agccgtgcgc tatctgttcg acacaatcgg tgcggcgccg
     2401 caggagttgc gcgcggttgt cgcgggcttc ggcgagcgcc gtatcgacga ataccgcgtt
     2461 ggcttcacgc gccgcggcag caacgatgtg atctacggcg tcgtatggcc actttacggc
     2521 cgcgagaacg gcgaacccgg tattgatgaa gaaccgcaag agaccgctgc gtcagaagac
     2581 ccgatcgaag aaatcgtcgc gctgctcaag gaaaccggtg tgacggacgt gcgccgccac
     2641 tcgggccgct tcgagccgga atattgtgat gactgcggtg tcccgctcta cgcagacccg
     2701 cttggcgaaa tcgtccacgc cgaaatgccg gaggacgcgg agcccgcgca accgcatttc
     2761 cactgagcca gcggcggcct tgagccgcgc cgtcgacacc aggaggcccc gccgcgcgcg
     2821 gggctttttt tacgtcattc ctcctatgcc ttttggacag gccgtcaaaa cgaagggaat
     2881 ttgtcatcat agtgccggtg gcgcatcgta gtgcgcccgg caccctcgcc ggcccgcacg
     2941 catcgcgcca cctcgtccga ttcatctgga gacatggcat gcgattctca atactcaact
     3001 cagacgcgga acgccgcgat ggactcaaag cactgctgcg gcaaatcgac cggctagcgc
     3061 gcttcaacga agcacaggat tggcgtcagg ttgaacgcac gctcaagcgc ctgcgtcccg
     3121 acttgctggt gatcgattgg caggactgga tgtcggtcga cgacgcccgt catctgctca
     3181 gccactaccc ggacctgcgc attgctgtgc tgaccgacga ggtctcgcca accgtcgtgc
     3241 ggaacctcat ggacgaaggt gtgctcggcg tggttccgcg cgaaaccgac ccatgcctga
     3301 tcgtgcgcgc tttcgagatg gttctgctag gcggtcacta tgtgccgccc ggcgcgttgg
     3361 cgctcaagcc gccgctaccg ccggacgccg ccatccggcc gttcgacgag aaaagtccgc
     3421 caccgcgtag aaccaaactt tccaacggcc tgtcgcctcg ccaggagcag atcatgcgct
     3481 gcgtccacat gggcagcacc aacaagatga ttgcgcgcac gctcggcatc agtgagggca
     3541 ctgtcaagat ccatctgacc agcatatttc agcaactcgg cgcgcccaat cgcgctgcgg
     3601 cggtcgcgct ctacaacggc tggctgtcag ctcatttgca ggtactgcgc aacggcgacg
     3661 caggtccggt ccgtccggta atggggcaag ccggcgtggt gcccttgcgg cgccgcacgc
     3721 tgcgacgctt ccagtatccg ttgcccgcga acgacacggc ggacccgctg ccaatggcag
     3781 ccgaaccgag cacgccttac ggcggtgtgc ccgccgggcc ggcggcggag caggtcacgc
     3841 aaggtccggc ataacctcag ccggcgcgcg cgccagaatc gggaggcgca ctttctggca
     3901 gcgtttgcgc cggctggcat ggaatccgct cacacctttt ctcgtccaac gagtaatatg
     3961 agatacatgg gtttcctcta cacaccgctt ccgttctggg tcgctgtcgg tggctggatc
     4021 gccactgcga tagtggt
//
LOCUS       DATASH010000373         1572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1835188,
            whole genome shotgun sequence.
ACCESSION   DATASH010000373 DATASH010000000
VERSION     DATASH010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1835188"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /gene="rfaD"
                     /locus_tag="VHV99_10430"
     CDS             complement(<1..689)
                     /gene="rfaD"
                     /locus_tag="VHV99_10430"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HEX3635889.1"
                     /translation="MIIVTGGAGFIGSNLIQQLNRAGERDILLVDNFAPASNLSGPKF
                     LNLAGAAYADYMDKREFRAGLKSGEFDTRRISTILHQGACSNTLEDDGQYMMDNNFTY
                     SKELLHFALNRRIPLVYASTAAVYGDSAAFTEVPANERPLNVYGYSKLVFDNYVRRLM
                     PEFKSTVVGLRYFNVYGPREQHKGRMASVIHHFMRQLKDTGTIKMFEGSGGYADGEQR
                     RDFVFVKDLAR"
     gene            complement(686..>1572)
                     /gene="rfaE2"
                     /locus_tag="VHV99_10435"
     CDS             complement(686..>1572)
                     /gene="rfaE2"
                     /locus_tag="VHV99_10435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HEX3635890.1"
                     /translation="TGISPHQLDELLGAGQAMIAEYGFEFLTVTMSEKGITVLRRKSE
                     GIPAVYHSPARAREVFDVSGAGDTVIATLTAGLAAGLQIESAVELANLAAGIVVGKVG
                     TVPIASHELVAALTPSSGLRAGEKILDLERIKLRVAEWRASGETIVFTNGCFDLLHVG
                     HIALLEDCRRFGSKLVLGLNADSSVCRLKGPTRPIVGERERARVMAALAAVDAVVLFE
                     QDTPLELIRALKPNVLVKGGDYTVETVVGHEDVIAAGGRVEIVPTVEGFSTSIIVKKL
                     TRQAAEEGLVIQGEEIKN"
BASE COUNT          310 a          520 c          439 g          303 t
ORIGIN      
        1 atccgcgcca ggtccttcac aaagacgaag tcgcgcctct gctctccatc ggcatagccg
       61 cctgagccct cgaacatctt aatcgtgccg gtatccttca actgccgcat gaagtggtga
      121 atgacgctcg ccatgcgccc cttgtgctgc tcccgcgggc catacacgtt gaagtagcga
      181 agccccacca ccgtgctctt gaactccggc atcaggcggc gaacatagtt atcgaagacc
      241 agcttcgaat acccatacac gttcagcgga cgctcgttcg caggcacctc cgtaaacgcc
      301 gcgctgtctc cgtagaccgc cgccgtcgac gcatacacca gcggaatcct gcgattcagc
      361 gcgaagtgca acagctcctt ggagtaagtg aagttgttgt ccatcatgta ctgtccatcg
      421 tcttcaagcg tgttcgagca cgcgccctga tgcagaatcg tgctgattct cctggtgtcg
      481 aactcgcccg acttcaggcc tgcgcggaac tcgcgcttgt ccatgtaatc ggcatacgcg
      541 gcgcccgcaa ggttcagaaa cttagggccg ctcaggttcg acgccggagc gaagttatcg
      601 accagcagaa tatcgcgctc gcccgcgcgg ttgagctgct ggatgaggtt gctgccgatg
      661 aaccctgcgc cgcctgtgac aataatcaat tcttaatctc ctcaccctgt atgaccagtc
      721 cctcttccgc tgcctgtcgg gtaagcttct tcacgatgat gctcgtggaa aagccttcga
      781 cggtgggaac gatctccacg cgcccacccg cggcgataac gtcctcatga ccgaccactg
      841 tttcgaccgt gtaatcgccg cccttcacca gaacattggg tttgagcgcg cggatcagct
      901 ccagcggcgt atcttgctcg aagagcacaa cagcatcgac ggcggccagg gcagccatca
      961 cgcgggcacg ttcgcgctct ccaacgatag gccgcgtcgg ccccttcagc cgacagacgg
     1021 aggagtccgc gttcagtccc agcaccagct tcgagccgaa gcggcggcaa tcttcgagta
     1081 gcgcaatgtg gccgacatgc agcagatcga agcagccgtt ggtgaagaca atcgtctccc
     1141 ctgaggctcg ccactcggcc acgcgcagct taatgcgctc caggtcgagg atcttctcgc
     1201 ccgctctcaa tccggagcta ggcgtcagcg ccgctaccag ttcatggctg gcgataggaa
     1261 cggtgccgac cttgcccacc acgattcccg cagccaggtt cgcgagttcg acggcgctct
     1321 caatctgaag cccggcggcg aggccggcgg tcagtgtagc aatcacggtg tcgcccgctc
     1381 ccgacacgtc gaagacctcg cgcgcacgag ccggcgaatg gtagacggcc gggataccct
     1441 ccgacttacg tcgaaggacg gtgattccct tctcgctcat cgtgaccgtg aggaactcga
     1501 agccgtactc ggcaatcatc gcctgcccgg cgccgagcag ctcatcgagt tgatgcgggg
     1561 aaatgccagt gg
//
LOCUS       DATASH010000374         4889 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3813654,
            whole genome shotgun sequence.
ACCESSION   DATASH010000374 DATASH010000000
VERSION     DATASH010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4889
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3813654"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="VHV99_10440"
     CDS             complement(<1..887)
                     /locus_tag="VHV99_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEX3635891.1"
                     /translation="MVYPSIRDWLFSVKTFAAAMIALYIGLALELPRPYWAMATVYIV
                     SNPFVGATRSKALYRTLGTALGASAAVLLVPPFVESPYLFSVIVALWTGTLLYLAVAD
                     RTARSYVFMLAAYTMPIIALPSVTNPAGVFDLAISRTEEITLGIVCASIVGSALFPSR
                     LAPTIIERTDAWFRDAAFYATETLSGRITGAAISGVRQRIAVTINGLELLLSQLAYDH
                     TRPDILARTHELRGRMQLLLPMTSALADPLIALYNSGRQTWPEGLEALLNDVIKWFEA
                     PLPAASRGIHPDLAADALRA"
     gene            complement(894..1355)
                     /locus_tag="VHV99_10445"
     CDS             complement(894..1355)
                     /locus_tag="VHV99_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX3635892.1"
                     /translation="MSNLHALRRTVSSTLVVAARKWRRTSHGVLAAFNVSEACATPLL
                     TASRLGEAVRQVTLAEYIGIEGPSLVRLLDQLCAAGLMRRDEDPEDRRAKTVVLTDEG
                     RAVTAKMEEELVTLRAKALKGISRSDLEATLRVLNAFTAEAAPPHDGGDFE"
     gene            complement(1514..3172)
                     /locus_tag="VHV99_10450"
     CDS             complement(1514..3172)
                     /locus_tag="VHV99_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3635893.1"
                     /translation="MEQRVLILAPFGRDADVIAEVLQKDARTSIACRDANVLAEALDA
                     GAGSALIAEEALADGHASRLFDWLEHQPAWSDFPFIMLAANRVGRRSARSLEVLERLG
                     NVVVLERPLNSETLRRAVGSALRARSRQYESRRHLAELIEAQEALVQLNDSLESRIAE
                     RTHELASANNRLMMEIHERAKVQAVLVQSQKMEALGQLTGGIAHDFNNLLNVIMVNSE
                     LIARVGSDDRIRAMAATVKRATERGAKLTGQLLTFSRNSNLDLKAVDVVTLLQGMRDI
                     ITVSLGSSIRYTTEFDCEEMWTHADANQLELAILNLAINARDAMPNGGQLAIHVKERT
                     APDQTLEEGRYIVLEVADTGSGIAPDVVSRVFDPFFTTKPIGKGTGLGLSQVYGIARQ
                     AGGTARLFSEERVGTTVEIWLPLRERVASQTEAVSDVEENAVGVKRVLVVEDDGEVRA
                     MLVECLRMLGYTVTEAADGQAGLNRLQADNPDLLMVDFAMPGMNGIDVIAAARKMRED
                     LPVILATGYADVDISGLAVKRCTILRKPFQLDDLARTVRLGLAA"
     gene            complement(3177..4694)
                     /locus_tag="VHV99_10455"
     CDS             complement(3177..4694)
                     /locus_tag="VHV99_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEX3635894.1"
                     /translation="MPNTPAAASPARLSSGIEGIDDILGGGFTPHRMYLVEGAPGTGK
                     TTLALQFLLKGVAEGQVGLYITLSETRDELISVADSHGWDLSRFTILELLSDEGLDPR
                     YEQTVLHPAEVELGETVRNVIEQVDELKPARIVLDSLSELSLLSQNPLRYRRQILALK
                     RYFATRECTVLLLDDNSSEPGDVQLHSIAHGVIALDNLVHDYGGNRRRMRVAKMRGIK
                     FREGYHDFTLDTGGIHVYPRLVAAEHHAAFKTDVLSTGTDGLDALLGGGLIPGTSALM
                     IGPSGVGKTTTVVSCLIAALERGERCVYYVFDETLTTLMIRCATLGMYLAPHVESGLL
                     TLRQIDPAEISPGEFASDLRNSVEKKGVKYVAIDSLNAYLQAMPGERYLLLQMHELLG
                     YLNQQGVVTMLVLGQHGIIGEVQNDLDISYLSDVVVLFRYFEHHGEVLTAVTAVKSRA
                     NAHERSIRQFRLGRGGVEVGEALRDFEGVLSGLPAYRGSTALLGAQDRLIDTSRQ"
BASE COUNT          871 a         1508 c         1604 g          906 t
ORIGIN      
        1 gcgcgcagcg cgtcggcggc gaggtcgggg tggatgccgc ggctggctgc gggcagcggc
       61 gcctcgaacc acttgatgac gtcgttgagc aatgcttcga ggccctcggg ccatgtctgc
      121 cggccgctgt tgtagagcgc gatcagcgga tcggccagcg ccgacgtcat gggcagcagc
      181 aactgcatgc gcccgcgcag ctcatgagta cgggccagaa tgtccggccg cgtgtggtcg
      241 taagcgagct ggctcagcag cagttccagc ccgttgatcg tgaccgcgat tcgctggcgg
      301 acccccgaaa tcgccgcgcc ggtaatgcgc ccggagagtg tttcggtcgc atagaacgcg
      361 gcgtcgcgaa accacgcgtc ggtccgctcg atgatggtgg gcgcgagccg gctgggaaac
      421 agcgcactgc cgacgatgct cgcgcacacg atgccgagtg tgatctcttc ggtgcggctg
      481 attgccagat cgaacacgcc cgcaggattg gtcaccgaag gcaaggcgat gatcggcatg
      541 gtgtaggccg ccagcataaa gacgtagctg cgcgcggtac ggtcggccac cgccagatac
      601 agcagcgtgc cggtccataa cgcgacgatc acgctgaaca gatacggcga ttcgacgaat
      661 ggcggcacca gcagcaccgc ggccgaggcg ccgagcgccg tgccgagcgt acgatagagc
      721 gccttggagc gggtcgcgcc gacaaacgga ttcgacacaa tgtagacggt ggccatcgcc
      781 cagtacgggc gcggcaactc gagcgcgaga ccgatgtaga gcgcgatcat cgccgccgca
      841 aaagtcttga cggagaacag ccagtctcga atggagggat agaccataaa ccgttactcg
      901 aaatccccgc catcgtgcgg tggcgctgcc tcggccgtga aagcgttcag cacgcgcaag
      961 gttgcttcga gatcgctgcg tgagatgcct ttcaaagctt tcgcccgcag cgtaacgagc
     1021 tcttcttcca tttttgccgt gacggcgcgg ccttcatcgg tgagcaccac ggtcttggcg
     1081 cgccggtcct ccggatcttc gtcacggcgc atcaggccgg ccgcgcacaa ctgatcgagc
     1141 agccgcacga gcgatggtcc ttcaataccg atgtattcgg ccagcgtgac ctgccgcact
     1201 gcttcgccga gacggctggc ggtgagcagc ggcgtggcgc acgcttcgga gacgttgaag
     1261 gccgcgagca cgccgtggct cgtgcgccgc cattttctgg cggcgacgac caacgtgctg
     1321 ctgacggtgc ggcgcaaggc atggagattc gacatggccg gtattgtatt tccaaaatat
     1381 tcgttagcaa actattgaat tgcgaatatt tgtgaagacg cgctatgtgt agtggctgta
     1441 gcggggggcg cccgctacag cccaaggcga gcttcagact gcgtgcactg aagggcgccc
     1501 ccgcggcttc ttttcaggct gcgaggccga gcctgacggt acgcgccagg tcatcgagct
     1561 ggaagggttt gcgcaagatc gtgcaacgct tgacggcgag gcccgaaatg tccacgtccg
     1621 cataaccggt cgcgaggatc accggcaggt cctcgcgcat cttgcgcgcc gcggcgatca
     1681 cgtcgatgcc gttcatgccg ggcatcgcga agtcgaccat cagcaggtcg gggttgtcgg
     1741 cttgcaatcg gttcagcccg gcctgtccgt cagcggcttc ggtcacggtg tagccgagca
     1801 tgcgcaaaca ctcgaccagc atcgctcgca cttcgccgtc gtcttcgacg accagcacgc
     1861 gcttcacgcc gaccgcattc tcctcgacgt cggataccgc ttcggtctgc gaggcgacgc
     1921 gctcgcgcaa cggcagccag atctccaccg tggtgcccac acgctcttcg ctaaagaggc
     1981 gtgcggtacc gccggcttgc cgcgcgatgc cgtagacctg gctcaagccg aggcctgtgc
     2041 ccttgccgat cggcttggtc gtgaagaatg gatcgaacac gcgcgagacc acatcggggg
     2101 cgatccccga gccggtgtcg gcgacttcga gcacgatata ccggccctcc tcgagcgttt
     2161 gatcgggggc cgtgcgctct ttcacatgaa tggcgagctg gccgccattg ggcatcgcat
     2221 cgcgtgcatt gatcgccaga ttgaggatgg ccagttcgag ctgattggcg tcggcatggg
     2281 tccacatttc ctcgcaatcg aattcggtgg tgtaacgaat gctcgagccg agcgacacgg
     2341 tgatgatgtc gcgcatgcct tgcagcaagg tcaccacgtc caccgctttc agatcgagat
     2401 tgctgttgcg cgagaaggtc aacaactgtc ccgtcagttt cgcgcctcgc tcggttgcac
     2461 gtttgacggt ggcggccatg gcgcgaatgc gatcgtcgct gcccacccgc gcgatcaact
     2521 ccgagttgac catgatgacg ttcaacagat tgttgaagtc gtgcgcgatg ccgccggtga
     2581 gctgcccgag cgcttccatt ttctgcgatt gcaccagcac ggcctgcact ttggcgcgct
     2641 cgtgaatctc catcatcagc cggttgttgg cggacgcgag ctcgtgcgtg cgctcggcga
     2701 tgcggctctc cagcgaatcg ttgagttgca ctagcgcctc ttgcgcctcg atcagctcgg
     2761 ccagatggcg gcgcgactcg tactgccgcg agcgcgcccg cagcgccgag cctaccgccc
     2821 gccgcagcgt ttccgagttc agcgggcgct cgaggaccac gacgttgccg agccgctcga
     2881 gcacttcgag gctgcgagcc gaacggcgtc cgacgcgatt ggcggcgagc ataatgaagg
     2941 ggaagtcgga ccacgccggc tgatgttcga gccagtcgaa cagacgcgat gcatgaccgt
     3001 ccgcgagggc ctcctcggcg atcagcgcgc tgccggcgcc ggcgtcgagc gcttcggcga
     3061 ggacattggc gtcgcggcat gcaatggagg tacgtgcgtc cttctgcagg acttcggcaa
     3121 tcacatcggc gtcgcggcca aacggcgcca ggataaggac gcgttgctcc atatcgttac
     3181 tgccgggacg tgtcgatgag acgatcctgc gcgcctagca gggcggtgct gccgcgatag
     3241 gctggcaggc cggagagcac gccttcgaag tcgcgcaatg cctcgccgac ttccacgccg
     3301 ccgcggccga gccggaactg gcggatcgag cgttcgtgcg cgttggcgcg gctcttcacg
     3361 gcggtcacgg ccgtcagcac ctcgccatga tgttcgaaat agcggaacag cacgaccacg
     3421 tcactcaaat agctgatgtc gagatcgttc tgcacctcgc ctatgatgcc gtgctgaccg
     3481 agcaccagca tcgtgacgac gccttgctgg ttcagatagc cgagcaattc atgcatctgc
     3541 agcagcagat agcgctcacc gggcatggcc tggagatagg cattcagact gtcgatggcg
     3601 acgtacttca cgcctttctt ttcgaccgag ttgcgcaagt cgctcgcgaa ttcgccgggc
     3661 gagatttctg ccggatcgat ctggcgcagc gtcagcaagc cgctttcgac gtgcggcgcg
     3721 aggtacatgc cgagcgtcgc gcagcggatc atcagcgtgg tgagggtttc gtcgaacacg
     3781 tagtagacgc agcgctcacc acgttcgagc gccgcgatca ggcacgacac caccgtggtg
     3841 gttttgccga cacccgatgg gccgatcatc agcgcgctgg tgccgggaat caggccgccg
     3901 ccgagcagcg cgtcgaggcc atccgtgccg gtgctgagca cgtcggtctt gaacgccgcg
     3961 tgatgctcgg ccgccaccag ccggggataa acgtggatgc cgccggtgtc gagcgtgaag
     4021 tcgtgatagc cctcgcggaa cttgatgccg cgcatcttgg cgacgcgcat gcgccgccgg
     4081 ttgccgccgt agtcgtgcac gagattgtcg agggcgatca cgccatgcgc gatgctgtgc
     4141 aactggacat cgccgggctc cgacgaattg tcgtcgagta gcagcaccgt gcattcgcgg
     4201 gtggcgaaat agcgtttgag cgcgagaatc tgccggcggt aacgcaacgg gttctgcgac
     4261 aacagactta actcggagag actgtcgagc acgatgcgcg cgggcttgag ttcgtcgacc
     4321 tgctcgatga cgttgcgcac ggtctcgccg agctcgactt cggccggatg caacaccgtt
     4381 tgctcgtagc gcggatcgag gccttcgtcg gacagcagct caagaatcgt gaaccggctg
     4441 aggtcccagc cgtggctgtc ggcgaccgag atcaactcgt cgcgtgtctc ggacagggtg
     4501 atgtacaagc ccacctggcc ttctgccacg cccttgagta agaattgcaa cgccagggtc
     4561 gttttacctg tgccgggcgc gccttcgaca aggtacatgc ggtggggggt gaagccgcct
     4621 ccgagaatgt cgtcgatacc ttcgattccg gaggagaggc gggccggcga agcggcagca
     4681 ggtgtgttgg gcatggcgta gcgtttgcgt cgatgaaggt gttgtcttcc gagccggtcc
     4741 ggttcgcgaa cgcaatttgt atgcctactg gcgttgcctc gctggtccaa cctggtttca
     4801 ccggtttcag gcctttgcgg cttcgttgca aaagtctcgg agcagttagc cgcgaacgcg
     4861 ctccttcacc agttcgataa agcagggac
//
LOCUS       DATASH010000375         2096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4408144,
            whole genome shotgun sequence.
ACCESSION   DATASH010000375 DATASH010000000
VERSION     DATASH010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4408144"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="VHV99_10460"
     CDS             <1..640
                     /locus_tag="VHV99_10460"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase domain-containing
                     protein"
                     /protein_id="HEX3635895.1"
                     /translation="RDFLTPHAAYEDREGDFELVAKINGNLWRADIGHSPLDVVAWHG
                     NYAPYKYDLRRFNTIGSISFDHPDPSIFLVLQSQSDTPGVDTIDFVIFPPRWLAAEDT
                     FRPPLFHRNVASEFMGLVHGVYDAKAEGFVPGGASLHNCMSGHGPDADTFEMASHSDT
                     STPKKVGDTMAFMFETRTLIKPTRFALETAQLQAHYYECWQGLKKHFNPEQR"
     gene            637..1965
                     /gene="fahA"
                     /locus_tag="VHV99_10465"
     CDS             637..1965
                     /gene="fahA"
                     /locus_tag="VHV99_10465"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HEX3635896.1"
                     /translation="MSALSDLQATLDPSRKSWVGSANDSTNDFSIQNLPFGIFSDRTN
                     PAQRVGVAIGDEIVDLHVLKTAGLLKFPITPAGQFDARHDRVFEQRTLNDFISLGRDA
                     WRSVRIQLSNLLARDTATLRDDTALRSKALVRQSDAQLHLPVQIPGYTDFYSSKEHAT
                     NVGSMFRDPKNALLPNWSEMPIGYNGRASSVVVSGTPVRRPNGQLKLPDQERPVFGAC
                     RKLDIELETGFVIGAGNVLGEPIACADAEAHIFGMVLLNDWSARDIQQWEYVPLGPFN
                     AKTFATTISPWIVTLDALEPFRVDQPTQEPQPLPYLRHDGKHAFDISLEVTLRPQAAK
                     QATTISRTNFKHMYWTMAQQLAHHTVSGCNTRVGDLMGSGTISGLTADSCGSLLESTW
                     NGKNPLELKEGGTRAFIEDGDELTLAGWCQGDGYRVGFGTCVGAILPARG"
BASE COUNT          424 a          625 c          663 g          384 t
ORIGIN      
        1 gcgcgacttc ctcacgccgc atgccgccta tgaagaccgc gaaggcgact tcgaactcgt
       61 cgccaagata aacggcaatc tgtggcgcgc ggacatcggt cattcgccgc tcgacgtcgt
      121 ggcatggcac ggtaactacg cgccgtacaa atacgatctg cgccgcttca acacgattgg
      181 ctcgatcagc ttcgaccatc cggacccgtc gattttcctg gtgctgcaat cgcaaagcga
      241 tacgccgggc gtcgatacga tcgacttcgt gattttcccg ccgcgctggc tcgccgccga
      301 ggatacgttc cgtccgccct tgttccatcg caacgtcgcg agcgaattca tgggcctcgt
      361 gcatggtgtg tacgacgcga aggccgaggg cttcgtgccg ggcggcgcga gtctgcacaa
      421 ctgcatgtcg ggtcatgggc cggacgcgga caccttcgag atggcctcgc acagcgatac
      481 gtcgacgccg aagaaagtcg gcgacacgat ggccttcatg ttcgaaacgc gcacgctgat
      541 caagccgacc cgtttcgcgc tcgaaaccgc gcaactgcag gcgcattact acgagtgctg
      601 gcaaggtctc aagaaacatt tcaacccgga gcaacgatga gcgcattgag cgatcttcag
      661 gcgacgctcg atccgtcgcg caagagctgg gtcgggtcgg cgaacgattc gactaacgat
      721 ttttcgatcc agaacctgcc gttcggcatt ttcagcgaca ggaccaaccc cgcgcagcgc
      781 gtgggcgtcg cgattggcga cgagatcgtc gacttgcatg ttctcaagac cgccggcttg
      841 ctcaagttcc cgatcacacc ggccgggcaa ttcgacgcga ggcatgaccg ggtattcgag
      901 cagcgcaccc tcaacgattt catttccctc ggccgcgatg catggcgcag cgtgcgcatc
      961 cagctgagca acttgctggc gcgcgacacc gcgaccttgc gcgatgacac cgcgttgcgt
     1021 tcgaaggcac tcgtgcgtca gtccgatgcg caactgcatt tgcctgtgca gattccgggc
     1081 tacaccgatt tctattcgtc aaaggagcac gcgacgaatg tcggctcgat gttccgcgat
     1141 ccgaagaatg cgctgttgcc gaactggtcg gagatgccga tcggttacaa cggacgcgcg
     1201 tcgtcggtgg tggtgagcgg gacgccggta cggcggccga atggccaact gaaactgccg
     1261 gatcaggagc gtccagtatt cggcgcatgt cgcaagctgg atatcgaact ggagacgggc
     1321 ttcgtgatcg gtgccggcaa tgtactcggc gagccgattg cctgtgccga tgccgaagcg
     1381 catatcttcg gcatggtatt gctgaacgac tggagcgcgc gcgatatcca gcaatgggaa
     1441 tacgtgccac tcggcccgtt caacgcgaag acatttgcga cgacgatctc gccgtggatc
     1501 gtgacgctcg acgcgctcga acctttccgc gtcgatcagc cgacacagga accgcagccc
     1561 ctgccgtacc tgcgccacga cggcaagcat gcgttcgaca tctcgctgga agtgacgctg
     1621 cggccgcagg cggcgaagca ggcaaccacg atctcgcgaa ccaacttcaa gcacatgtac
     1681 tggacgatgg cgcaacaact cgcacatcac acggtatcgg gctgcaatac gcgggttggg
     1741 gatctgatgg gctcgggcac gatcagcggc ctgacggcag actcgtgcgg cagcctgctg
     1801 gagtcgacgt ggaacggcaa gaacccgctg gagttgaagg aaggcggcac acgggcgttc
     1861 atcgaggacg gcgacgaact gacattggcc ggctggtgcc agggtgatgg ctaccgcgtg
     1921 ggattcggta cctgtgtggg ggcgatcctg ccggcccgcg gttaaagcga agagggtgga
     1981 tgaaaggccg gcagaatgcc ggcttttttt attgcgcgcg gtgctcggcg cgccgccagt
     2041 gaagcaaata ggtggagaag ctcgctgaaa gagaatcacg ctgccgagta aggtgg
//
LOCUS       DATASH010000376         2213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3615277,
            whole genome shotgun sequence.
ACCESSION   DATASH010000376 DATASH010000000
VERSION     DATASH010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3615277"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="VHV99_10470"
     CDS             complement(<1..723)
                     /locus_tag="VHV99_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3635897.1"
                     /translation="MSTLFSSRFYKRIERGIVPVLLLAGWEAFSRSGILPAALLPAPS
                     QVALAWADWIFSTDGNTQTYSGHWLFDIAASSGRVFAGFAIATVLGVAIGMAIGWSRS
                     IESLLEPTLQTLRPVPPVSWIPLAIIWFGIANKPAIFLVFMGSFFPVLLSTIHGVKTC
                     DRNLLRAGAMTGGTSSMLLRHIVFPAALPSIFSWLRIAIGSAWMLTVTAEMVAVKSGV
                     GYVLWDSYYFLRYDIVIASMVSI"
     gene            complement(737..1777)
                     /locus_tag="VHV99_10475"
     CDS             complement(737..1777)
                     /locus_tag="VHV99_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3635898.1"
                     /translation="MRFIRPSLAALQRAARVLLAPLVVATALMPTVGHAKDADPINLG
                     YAKCAHCTPLSLTPQYATGVKLTATSFNTGNDVLTALLSKSIDVAQVTYLHYATALDK
                     GFKVVGISGQINGGSQVLIANNLPVQPGDWDGLKKLIMQYKKDGKPFRVAASRGNAQD
                     IHMRGAFLTHGIDINKDVQFVNIPNPADHVQALRRGEVELITSVEPFATQIREVKAAK
                     FFAFPYDQAAGKLTNLIVTRPDVIESNPQGVLETVRAIVKVDDMMAANKPLFVNTIQK
                     VTGLDKTIATGAADNLYPDYKMYRASAVSIAKMMHDLKYVNSDVSDAVQKNLDYSFLE
                     KVTGKPKTELGY"
BASE COUNT          437 a          656 c          708 g          412 t
ORIGIN      
        1 gatgctcacc atcgacgcga tcacgatgtc gtagcgcagg aagtaatacg aatcccacag
       61 cacgtagccc acgccgctct tcaccgcgac catctcggcc gtcaccgtca gcatccatgc
      121 ggagccgatc gcgatgcgca gccacgagaa gatgcttggc aacgcagcgg gaaatacgat
      181 gtgccgcagc aacatcgagg acgtgccgcc cgtcatcgcg ccggcgcgca gcaggttgcg
      241 gtcgcaggtc ttcacgccgt gaatcgtgct gagcagcacc gggaagaacg accccatgaa
      301 gacgagaaag atcgccggct tgttggcgat gccgaaccag atgatcgcga gcggaatcca
      361 cgagacgggc ggcaccgggc gcagcgtctg cagagtcggt tcgagcagcg attcgatcga
      421 gcgcgaccag ccgatcgcca tgccaatggc gacgcccagc accgtggcga tcgcaaagcc
      481 cgcgaacacg cggccggaac tggccgcgat atcgaagagc cagtggccgg agtaggtctg
      541 cgtgttgccg tcggtgctga aaatccagtc ggcccaggcg agcgcgacct gcgacggcgc
      601 cggcagcaac gcggccggca ggatgcccga gcgggagaac gcctcccagc cggccagcag
      661 cagcaccggc acgatgccgc gctcgatccg cttgtagaaa cgtgaggaaa acagggttga
      721 catggcgcgg tccggatcag taaccgagtt cggtcttggg tttgccggtc actttctcga
      781 ggaagctgta gtcgaggttc ttctgcacgg catcgctgac atccgagttc acgtacttca
      841 ggtcgtgcat catttttgcg atcgatacag cggaggcgcg atacatcttg tagtccggat
      901 agaggttgtc ggccgcgccg gtggcgatgg tcttgtcgag gcccgtcact ttctggatcg
      961 tattgacgaa gagcggcttg ttggcggcca tcatgtcgtc caccttgacg atcgcgcgca
     1021 cggtctccag cacgccttgc ggattgctct cgatcacatc cgggcgcgtg acgatcaggt
     1081 tggtcagctt gccggccgcc tggtcatacg gaaacgcgaa gaacttggcc gccttgacct
     1141 cgcggatctg cgtggcgaac ggctcgaccg aagtgatcag ctcgacttcg ccgcggcgca
     1201 acgcctgtac gtggtccgcc ggattcggaa tgttgacgaa ctggacatcc ttgttgatgt
     1261 cgatgccgtg cgtgaggaac gcgccgcgca tgtgaatgtc ctgcgcgttg ccgcgcgagg
     1321 ccgccacgcg gaacggcttg ccgtccttct tgtactgcat gatgagtttc ttcaggccgt
     1381 cccagtcgcc cggctgcacc ggcaggttgt tggcgatcag cacctgcgaa ccgccgttga
     1441 tctggccgga gatgccgacc accttgaagc ccttgtcgag cgccgtcgca tagtgcagat
     1501 aggtgacctg cgcgacgtcg atgctcttcg agagcaaggc ggtcagcacg tcgttgcccg
     1561 tgttgaacga ggtcgccgtg agtttgacgc cggtggcgta ctgcggcgtc agcgacaatg
     1621 gcgtgcagtg cgcgcacttg gcgtagccga ggttgatcgg atcggcatcc ttggcgtggc
     1681 cgaccgtcgg catgagcgcg gtggcaacga cgaggggcgc gagcagcacg cgtgcggcgc
     1741 gctgcagggc ggcgagagac gggcggatga atcgcatcag aggagctccg ttattgtcac
     1801 aaaatgaaat acatttgtga caaataaagc aatagtcgtg ccactcgatt gtcaggccgt
     1861 ctccggcgac ttcgagcgat attcctgtac ctggctcaag tccgcatggc cgcgcgcgtc
     1921 cgcgctataa gtacggtgag gcaaaacgac gcgattcata tcgccagccg gccggcgaaa
     1981 ccggattctc gctgcgcgca caaacctggc accgttccgg tgcctcttgg gatacgtaag
     2041 cggtgccggt gtgcgctgat ggtgcaaatt cgaacaatta caattatttt tgtcacaata
     2101 tataattgta ttgacacaaa atgaccgttg gtcagtcgag gaaacctagc gtgttaaccg
     2161 acaaagcatc cctccgcccg cgtaaggcgc ggccggccgc ccagatcctg gat
//
LOCUS       DATASH010000377         8616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3813981,
            whole genome shotgun sequence.
ACCESSION   DATASH010000377 DATASH010000000
VERSION     DATASH010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 8616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8616
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3813981"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(226..753)
                     /locus_tag="VHV99_10480"
     CDS             complement(226..753)
                     /locus_tag="VHV99_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HEX3635899.1"
                     /translation="MSTRKMSRVLAVAATALAIGTGAAYAAQPAAHGGPGGWHGHFMK
                     ELTQLHDQLKLNADQEKQWQAALDTMKQNHEAMRANHKQIKDQFKAAQQQPILDLNAM
                     AAAHQQVEQKDAQLRQQTSDAWLKFYNGLNDQQKTTVSTALKQRFAKMEARHEKMHDR
                     WEHRKGSASAPAANQ"
     gene            973..1698
                     /locus_tag="VHV99_10485"
     CDS             973..1698
                     /locus_tag="VHV99_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013699030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3635900.1"
                     /translation="MATQILVVDDDVELRDLLRDYLARQGIEVSVLHDAGTLERRLER
                     ERPDLIVLDLMMPGVDGLTALRKLRASGDDIPVIMLTARADDVDRIVGLELGADDYLG
                     KPFNPRELLARVQAVLRRRRTLPSAAAPEQREPFNFGRFTLDFQSRTLHLEDKPLTLS
                     GSEFALLKIFVNHPMRTLTRERLLELLHGPEYDGTDRGIDVQVWRLRRILETDPSTPR
                     FIQTVRGRGYVFVPDGEQHAPAH"
     gene            1682..2998
                     /locus_tag="VHV99_10490"
     CDS             1682..2998
                     /locus_tag="VHV99_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3635901.1"
                     /translation="MRRPIDSLFGRLALLVIAVLLLSHFAWYALMRLERSQLQTRYAV
                     EEAIFLVDSVRQHVARTPDLPLPSRVRLVDPTSPDVAPENADLPPPLERFVEDVRDRM
                     PSGTLVRVGSPGRPPTLWVRATTDRSWIVVPLQTLRPPRSLDRTVLWLAIIFAFAVMA
                     ALFAAWALQQPLRSLAQAVARFGRGLPVPPVPERGPRELRQLTHGFNQMVQEVARTEN
                     DRAVMLAGVAHDLKTPLARLRLRAEMMEEVKMRDGVVRDVDSMTHIVEQFLVFAHDGA
                     DRSEAVEVDGQCERVVRSYRAVAAGASAVRTELNAGPGFLLPAATLDRILSNLLDNAH
                     AYGAPPIVVATARTAQGFTLSVSDNGSGIAAQDLINASRPFVRLDPARGGNGHSGLGL
                     AIVERLVRRAGGEWEIGNHGGRGLRVLMSFPFEAVPRVMVASESVW"
     gene            complement(3019..3285)
                     /locus_tag="VHV99_10495"
     CDS             complement(3019..3285)
                     /locus_tag="VHV99_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635902.1"
                     /translation="MKGNLVIVCRDQDAHAFDHLLAEYGAFQTRLSSTAWYLKLAVAP
                     ELIQEEIIARLGKYTTHYIFEAETVTWNTVDSDAANALNTLFSD"
     gene            complement(3413..5104)
                     /locus_tag="VHV99_10500"
     CDS             complement(3413..5104)
                     /locus_tag="VHV99_10500"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX3635903.1"
                     /translation="MNRKTLRQATGPISFVLIVLVCWFVAGMVADRMVQQELDAALRA
                     QRQMSTSIVDNMAEVIASDLAMSRAIPATMAEMDVVQHALVQSQNYAANSAAAEPALR
                     TALLKDPRMVAVSNFLHDAQGFSGLDQIWLVNANGICVASSNAIDNPRTRESSFVGID
                     MRVRGYLTNALLGAFSEAYGVGRNSGEPGIFIAAPAYADGLLVGVVVAKVGIARLRHW
                     VAHAGTFVADDNGVIIMAHNSELEGHALPDSRVTKMSAAERMTIYRREILPDIQIQVD
                     TQVREQAPWVPTAVAGQLFGMSAQPAPSLYQARSGLNSGLSAHLVDPLAAWPELLRNH
                     KRDHLLVFLTLGGTVALAWVITVSYVRERRHHRATRDLAEQLQSANTLLSAEARHDAL
                     TGALSRRYFLDLLRHEIERAHAGNEPLCMAIADLDHFKQINDRFGHAAGDRALEHFVD
                     TCRAELRGADAIGRLGGEEFGLLLPATDLAGGREVVERLRLRLKAMPSPKLPPSVGLS
                     VSIGITELSPDDLPERIMSRADTALYAAKTGGRDRTEALPPDDTAPPARTVASVW"
     gene            5663..5839
                     /locus_tag="VHV99_10505"
     CDS             5663..5839
                     /locus_tag="VHV99_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635904.1"
                     /translation="MSVIGSRVSNANDLPRTRVFAMPDFRFPDRSSDRRAEAAAPFVF
                     LNARMTSIKKGESV"
     gene            5839..7590
                     /locus_tag="VHV99_10510"
     CDS             5839..7590
                     /locus_tag="VHV99_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX3635905.1"
                     /translation="MDFSFNLKRTANASAWRVLPNRWDFVAFPLIICIIAMAAIGFHE
                     TLAPMSTLKTQTISLDPANLPEYAMRTTLRMLAAMVASLTFTLVYGTLAAKSRRAGLV
                     LVPILDILQSVPVLGYISFTVTFFLALFPGRVLGAELAAIFAIFTSQAWNMTFSFYQS
                     LRTVPRDLDEVSRGFHLTSWQRFWKLEVPFSMPGLIWNMMMSMSGGWFFVVASEAITV
                     GNRTITLPGIGAYLAQAISDKNLHAIGWVILAMTVVILAYDQFLFRPLVAWADKFRME
                     NTSSGDAPESWLLDLIRRTRLIHRLLVPLGWMFARAARVPFRLPAFQGPRFQIPQSQK
                     SSRVGDILWAALVLIVTVYVVYRVFTYVRTGVSLDEVWHVLVLGLITLLRVVVLIAIA
                     SVIWVPAGVLIGLRPALAEKIQPLAQFLAAFPANLLFPVFVIAIVRFHLNPDIWLSPL
                     IVLGTQWYILFNVIAGASSYPNDYREAAKNFHIRGWQWWRQAMLPGIFPYYVTGAITA
                     SGGAWNASIVAEFVQWGDTKVAAHGLGAYIAQSTAAGDYPKIIVGIAVMSLFVTLFNR
                     LLWRPMYAYAEAKLRLD"
     gene            7687..>8616
                     /locus_tag="VHV99_10515"
     CDS             7687..>8616
                     /locus_tag="VHV99_10515"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3635906.1"
                     /translation="MQNPKAAMTATPIQTPPKPPRLGEEILRVKDVCRGFNKSQGELL
                     VLDDANLSLREGEIVGLLGRSGSGKSTLLRIIAGLIEPTDGEVTYMGKPLDGPAKGVA
                     MVFQTFALFPWLTVLQNVEAGLEAQGVGASERRTRALAAIDLIGLDGFENAYPRELSG
                     GMRQRVGFARALVVDPTLLLMDEPFSALDVLTAETLRTDLLDLLTQGRMPIKAVLIVT
                     HNIEEAVFMCDRILVLSSNPGRVIAEIKVPFKHPRNRLDPAFRRLVDEIYAKMTARQT
                     DEKTKKGLELHSWLPHVSTNLMAGLIETLAAAPY"
BASE COUNT         1394 a         2724 c         2806 g         1692 t
ORIGIN      
        1 tcattgctca ttgcccgcag cccgcagccc gcagcccgca gcccgcagcc cgcagcccgc
       61 agcccgcagc ccgcagcccg cagcccgcag cccgctgcga gcgcaacgcg caactcgagt
      121 cgaagagcgg gaagcgcgca aacaaaaacg ccacggacaa cctccgtggc gtttttgcat
      181 ccggacgacg gccgcgcgag ccgccttccg cctgcgacgt gccgcttact gattggcagc
      241 cggcgccgac gccgagccct tgcgatgttc ccagcgatcg tgcatcttct cgtgacgcgc
      301 ttccatcttc gcaaaacgct gtttcaacgc cgtgctgacg gtggtcttct gctggtcgtt
      361 caagccgtta tagaacttga gccacgcatc cgaggtttgc tgacgcagtt gcgcgtcttt
      421 ctgctcgact tgctggtgcg cggcggccat cgcgttcaga tccagaatcg gctgctgctg
      481 cgcggccttg aactgatcct tgatctgttt atggttggcg cgcatcgctt cgtggttctg
      541 cttcatggtg tcgagcgcgg cttgccattg cttttcctga tccgcgttca gcttcaactg
      601 gtcatgcagt tgcgtcagtt ctttcatgaa gtggccgtgc cagccgccgg ggccgccgtg
      661 agcggcgggt tgtgcggcat aggcagcgcc cgtgccgata gcgagggcgg tggcggcaac
      721 ggcgagaacg cgcgacattt ttctggtaga catgtaagac tccctgtaat ggaatagccg
      781 acgatgcaac cggtgattga ggacggcctg cattcggtaa gacacagcgt agacaactgc
      841 gtccggcgcc gtgttacgtg agttggtgcg gctattacgg gtcattacgt acgcctttca
      901 ccgtaacacc cggtaaccct tgatggccgt ctgaaatcga aaacccttct gctgcgcgtt
      961 aaacttcatc ccatggctac tcaaatactt gttgtcgacg acgacgtcga attgcgcgac
     1021 ctgctgcgcg actatcttgc ccgtcagggc atcgaagtct cggtgctgca cgacgcgggc
     1081 acgctcgagc gcaggctcga acgcgagcgt ccggatctga tcgtgctcga tctgatgatg
     1141 ccgggcgtag acgggctgac cgcgctgcgc aagctgcgcg cgtcgggcga cgacatccct
     1201 gtgatcatgc tcaccgcacg tgctgacgac gtcgatcgta tcgtcggcct cgagctcggc
     1261 gcggacgact acctcggcaa gccgttcaat ccgcgcgagc tattggcgcg cgtgcaggcg
     1321 gtgctgcggc gccgccgcac gctgccctcg gcggcggcgc cggagcaacg cgagccgttc
     1381 aacttcggcc gcttcacgct ggatttccag tcgcgcacgc tgcatctgga agacaagccg
     1441 ctcacgctgt cgggcagcga attcgcgctc ctgaagattt tcgtcaatca ccccatgcgc
     1501 acgctcacac gtgagcgcct gctcgaattg ctgcatggcc cggagtacga cggcactgac
     1561 cgcggcatcg acgtccaggt gtggcgcctg cgtcgcattc ttgaaaccga cccgtccacg
     1621 ccgcgcttca ttcagacggt gcgcggacgc ggctacgtgt tcgtgccgga tggcgaacag
     1681 catgcgccgg cccattgatt cgctgttcgg gcgcctggca ctgctagtca ttgcggtgct
     1741 gctgctgtcc catttcgcgt ggtacgcgct catgcggctc gagcgcagcc agctgcaaac
     1801 gcgctacgca gtcgaggaag ccatcttcct cgtcgactcg gtgcgccagc acgtcgcccg
     1861 cacgccggac ctgcccttgc cgtcacgcgt caggctggtc gatccgacaa gccccgacgt
     1921 ggcgccggag aacgccgacc tgcctccgcc gctcgaacgt ttcgtcgagg acgtgcgcga
     1981 tcgcatgccc tccggcaccc tggtgcgggt tgggtcgccg ggccgtccac ccacgctgtg
     2041 ggtgcgcgcc accacggatc gcagctggat cgtggtgccg ctgcagacat tgcgcccgcc
     2101 gcgttcgctc gaccgcacgg tgctgtggct ggcgatcatt ttcgcgttcg cggtgatggc
     2161 ggcgctgttt gccgcgtggg cgctgcagca gccgttgcgc tcgctcgccc aggcggtggc
     2221 gcgcttcggt cgcggcctgc cggtgccgcc ggtgcccgag cgcgggccgc gcgagttgcg
     2281 gcagttgacg catggcttca accagatggt tcaggaagtg gcgcgtactg aaaacgaccg
     2341 ggccgtgatg ctggccggcg tcgcgcacga cctgaaaacg ccgctggcgc gcttgcggtt
     2401 gcgcgccgaa atgatggaag aagtgaagat gcgtgacggc gtcgtgcgtg acgtcgattc
     2461 gatgacgcat atcgtcgaac agtttctggt gttcgcgcac gacggcgccg atcgcagcga
     2521 ggcggtcgaa gtagacgggc agtgcgagcg cgtggtgcgc agctatcgcg cggtggcggc
     2581 gggtgcgtcc gcggtgcgga ccgagctcaa cgcagggccc ggtttcctgc tgcccgcggc
     2641 aacgctcgac cggatcctgt cgaacctgtt ggacaacgca cacgcctacg gcgccccgcc
     2701 gatcgtcgtc gccacggcgc gcacggcgca gggtttcacg ctgtcggtga gcgacaacgg
     2761 cagcggtatc gccgcgcagg acctgatcaa tgcgagccgg ccgttcgtgc ggctcgaccc
     2821 ggcacgcggc ggtaatggtc atagcgggct agggctcgcg atcgtcgagc gtctggtgcg
     2881 ccgtgcgggc ggcgagtggg agatcggcaa ccacggcggc cgtggcttgc gggtcttgat
     2941 gagctttccg ttcgaagcgg tgccgcgtgt catggtggcc tcggaaagcg tctggtaaag
     3001 ctcgggtagg accctccact agtctgaaaa cagtgtgttc aacgcattcg ccgcgtcgct
     3061 atccaccgta ttccacgtga ccgtttcggc ttcgaaaata tagtgcgtgg tgtacttgcc
     3121 aagacgggcg attatttctt cctgaatcaa ttccggcgcg acagccagtt tcagatacca
     3181 cgcagtcgac gacaagcgcg tctggaacgc gccatattcg gccagcagat ggtcgaatgc
     3241 gtgagcatcc tgatcgcgac agacgatgac caaatttccc ttcatgcgaa tctcccgtta
     3301 gagcggtagc ggcaacctcg atatcagggt tgatgatacc tgcttcgtta cgggacgccc
     3361 gaggccaaga cttgctttgc atcagctacc gcacttatcc ggggcactgg cctcaccaga
     3421 cgctggccac tgtccgcgcg ggcggcgcgg tgtcgtcggg cggcagggct tcggtgcggt
     3481 cgcggccgcc cgtcttcgcc gcgtagagcg ccgtgtcggc gcggctcatg atgcgctcgg
     3541 gcagatcgtc gggcgagagt tcggtgatgc cgatgctcac gctcaagccc accgagggcg
     3601 gcaacttcgg cgaaggcatt gccttcaggc gcaggcgcaa gcgctcgacg acttcgcggc
     3661 ctcccgccag atccgtggcc ggcagcaaca agccgaattc ttcaccgccc agccggccga
     3721 tcgcatcggc gccgcgcaac tcagcccggc aggtgtcgac gaaatgttcg agggcgcggt
     3781 cgccggcggc gtggccgaag cggtcgttga tctgcttgaa gtggtcgagg tcggcgattg
     3841 ccatgcacag cggctcgttg cccgcatgcg cgcgttcgat ctcgtggcgc agcaggtcga
     3901 ggaagtaacg ccgcgacagt gcgccggtca gcgcatcgtg gcgcgcctcg gccgagagca
     3961 gtgtgttggc cgattgcaat tgctcagcaa ggtcgcgcgt ggcgcgatgg tgacgccgct
     4021 cacgcacata cgacacggtg atgacccacg caagcgcgac ggtgccgccc agcgttagaa
     4081 acaccagcag atgatcgcgc ttgtgattac gcaatagctc gggccacgcc gcgagcggat
     4141 cgaccagatg cgccgacaat cccgagttca agccgctgcg tgcctgatag agcgacggcg
     4201 ccggctgcgc gctcatgccg aacagctgtc cggcgacagc ggtgggcacc cagggcgcct
     4261 gctcgcgcac ctgcgtgtcg acctggatct gtatatcggg gaggatttcg cggcgataga
     4321 tggtcatgcg ctcggcggcg ctcatcttcg tgacgcgcga atccggcagc gcatggcctt
     4381 cgagttcgct gttgtgcgcc atgatgatga cgccgttatc gtcggcgacg aaggtgccgg
     4441 catgagcgac ccagtggcgc aaccgcgcga tgcccacctt ggccaccacg accccgacca
     4501 gcaggccgtc ggcatacgcc ggcgcagcga tgaagatgcc cggctcgccg ctattgcggc
     4561 ccacaccata ggcttccgaa aacgcgccga gcagggcatt cgtcaggtag ccgcgaaccc
     4621 gcatgtcgat gccgacaaag gagctttccc gggtacgcgg gttgtcgatg gcgttgctcg
     4681 atgcgacgca gatgccgttg gcgttgacga gccagatctg atcgagcccg gagaagcctt
     4741 gcgcgtcgtg caggaagtta ctcaccgcga ccatccgcgg gtcttttaac aacgcggtgc
     4801 gcagggcggg ttccgccgct gcgctgttcg cggcataatt ctgggattgc accagcgcgt
     4861 gctggaccac gtccatttcg gccatggttg cagggatggc gcgagacatc gcgaggtcgc
     4921 tggcgatcac ctccgccatg ttgtcgacaa tcgacgtgga catttgccgc tgtgcgcgaa
     4981 gggctgcgtc cagctcttgc tgtaccatcc ggtccgcgac catgccggcc acgaaccagc
     5041 aaacgagcac gatcagcacg aagctgatcg gtccggttgc ctggcgcaag gttttcctgt
     5101 tcatcgaccc tctgatccgt ctggctgtga catgcaactg gctcattcgt ggcagcgcgc
     5161 tggcgctatt tcgcttctgt gcaaacggct ggcaacacgc ccctggtgtg agcctcgctg
     5221 tggccccggc gtaggcgcaa gcggcgtgcc gtctcctgat tttaaccgtc atggggcctg
     5281 acgccaccgt tattgcggga atgcgaagtg tgtcagtttc gcccatcaga ttcttaacgg
     5341 cagcgcatca gctttctgaa tggctgctcg ggttttccag ggtttgaaac gcggctccgg
     5401 ccgctattgc aacggcgggg acggggctgg cgcgggacag tggttgccac gctgcagcgt
     5461 gcgtcggcgc cggccggcgg ccgttgctgg tgcgtgcaca gcgatttgcc gctatttgcc
     5521 gcgaaaggtt gtgcctgaaa cataggatgg tgtagtgtcg tgccgtccaa aacaaaggaa
     5581 agctcgccag ccttcgggcg gcgcctgcgg cgacaggagc ccggcttgac ggtttgcggt
     5641 cccgggcggc tcgcccacgg acatgtccgt catcggttca cgtgtctcca acgctaacga
     5701 tttaccgcgc acacgcgttt ttgccatgcc tgatttccgc tttccggacc gatcttcaga
     5761 ccgccgcgcc gaagccgcag cccccttcgt gtttttgaat gcccgtatga ccagtattaa
     5821 aaaaggggag agcgtgtgat ggatttcagc ttcaatctga agcgcaccgc caatgcgtcg
     5881 gcatggcggg tgctgcccaa tcgctgggat ttcgtcgcct tcccgctgat catctgcatc
     5941 atcgcgatgg ccgcgatcgg ctttcacgag accttggcgc ccatgtcgac gctcaagacc
     6001 cagacgattt cgctcgaccc ggcgaatctg cccgagtacg ccatgcgcac cacgctgcgc
     6061 atgctggcgg caatggtcgc ctcgctgacc ttcacgctgg tgtacggcac gctggcggcc
     6121 aagagccgcc gcgccggcct cgtgctggtg ccgattctcg acatcctgca gtcggtgccg
     6181 gtgctcggct acatttcgtt tacggtcacg ttcttcctcg cgctgttccc gggccgtgtg
     6241 ctcggcgccg agctggcggc gatcttcgcg atcttcacga gccaggcgtg gaatatgacc
     6301 ttcagcttct atcagtcgtt gcgcacggtg ccgcgcgatc tggacgaagt ctcgcgcggt
     6361 tttcatctga cctcgtggca gcgcttctgg aagctcgaag tgccgttctc gatgccgggc
     6421 ctcatctgga acatgatgat gtcgatgtcg ggcggctggt tcttcgtggt tgcgtcggaa
     6481 gccatcacgg tcggcaacag gacgatcacg ctgccgggta ttggcgctta tctcgcgcag
     6541 gcgatctcgg acaagaatct gcatgcgatc ggctgggtga tcctcgccat gaccgtcgtg
     6601 attctcgcct acgaccagtt cctgttccgc ccgctcgtcg catgggccga caaattccgc
     6661 atggagaaca ccagttcggg cgacgcgccg gaatcgtggc tgctcgacct gatccgccgc
     6721 acgcggctga ttcaccgtct gctggtgccg ctcggctgga tgttcgcgag ggccgcgcgc
     6781 gtgccgttcc gtctgccggc gttccagggg ccgcggttcc agattccgca gagccagaag
     6841 agttcgcgcg tcggcgacat tctgtgggcg gcgctggtgc tgatcgtcac tgtgtacgtg
     6901 gtgtatcgcg tgttcacgta tgtgcgcacc ggcgtctcgc tcgacgaggt ctggcatgtg
     6961 ttggtgcttg ggctcattac gctgctgcgg gtggtggtgc tgatcgcgat cgcttccgtg
     7021 atctgggtgc cggccggcgt gttgatcggc ttgcggccgg cgctcgcgga gaagatccag
     7081 ccgctcgcgc aatttctcgc cgcgttcccg gcgaacctgc tgttcccggt gttcgtgatc
     7141 gcgatcgtac gcttccatct gaacccggac atctggctgt cgccgctgat cgtgctcggc
     7201 acgcagtggt atatcctgtt caacgtgatt gccggcgcga gctcgtatcc gaacgactac
     7261 cgcgaagcag ccaagaactt ccatatccgc ggctggcagt ggtggcgcca agccatgctg
     7321 ccgggcattt tcccgtacta cgtgaccggc gcgatcaccg cgtcgggcgg cgcgtggaac
     7381 gcgagcatcg tcgcggaatt cgttcagtgg ggcgacacga aggtggccgc gcacggcctc
     7441 ggcgcctata tcgcgcaatc caccgcggcg ggcgactatc cgaagatcat tgtcggcatt
     7501 gccgtgatgt ctctgttcgt gaccctgttc aaccgtctgc tgtggcgccc gatgtacgcc
     7561 tacgccgaag ccaagctccg gcttgattga gcgccccgaa cctgtcgctt cgcgctgggc
     7621 ccccgaaggg ggtaatgact tgggggcgac ccggcgtttt gttgagagaa atgaaggcta
     7681 aacgcgatgc aaaatcctaa agctgctatg accgccacgc cgatccagac accgccgaag
     7741 ccgccgcgcc tcggcgagga gatcctgcgc gtcaaggacg tgtgccgcgg gttcaacaag
     7801 tcgcagggcg agctgctcgt cctcgacgac gcgaatctgt cgctgcgcga gggcgagatt
     7861 gtcggtttgc tgggccgttc gggttcgggc aagtcgacac tgctgcgcat catcgccggt
     7921 ctgatcgagc cgaccgacgg cgaagtcacc tatatgggca agccgctcga cggcccggcc
     7981 aagggtgtgg cgatggtgtt ccagaccttc gcgctgtttc cgtggctgac cgtgctgcaa
     8041 aacgtggaag cgggactgga agcgcaaggc gtcggcgcga gcgagcggcg cacgcgcgcg
     8101 cttgcggcaa tcgacctgat cggtctggac ggtttcgaaa acgcttatcc gcgtgagttg
     8161 tcgggcggca tgcgccagcg cgtcggcttt gcgcgcgcgc tggtggtcga tcccacgctg
     8221 ctgctgatgg acgagccgtt ttccgcgctc gacgtgctga ccgccgaaac gttgcgtacc
     8281 gacctgctcg atctgttgac gcaaggccgc atgccgatca aggccgtgct gatcgtcacg
     8341 cacaatatcg aggaagcggt gttcatgtgt gaccgcattc tggtgctgtc gtcgaatccg
     8401 ggccgcgtga tcgccgaaat caaggtgccg ttcaagcatc cgcgcaatcg tctggacccg
     8461 gcgttccgcc ggctggtgga cgaaatctac gcgaagatga ccgcgcgcca gaccgacgaa
     8521 aagaccaaga agggtctcga gctgcatagc tggctgccgc acgtgtcgac caacctgatg
     8581 gccggtctga tcgaaacgct ggctgcggcg ccgtac
//
LOCUS       DATASH010000378         7287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2627579,
            whole genome shotgun sequence.
ACCESSION   DATASH010000378 DATASH010000000
VERSION     DATASH010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7287
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2627579"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="VHV99_10520"
     CDS             complement(<1..529)
                     /locus_tag="VHV99_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3635907.1"
                     /translation="MANVAEARGARAAGMSFRVTMSLIGGVGVLLLMLPTLIVLVTSF
                     TSGYSLKFPPPGFSLRWYQALVFDSPEIIDALTLSLKLAALATAIATVLAVGAAVALA
                     RRRSLAARVLDSFFMAPLTVPSLALGLGILVLFNLESAGLSFYSLLAGHIALCAPYIL
                     YTPSASYAQLDPALLD"
     gene            complement(522..1397)
                     /locus_tag="VHV99_10525"
     CDS             complement(522..1397)
                     /locus_tag="VHV99_10525"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3635908.1"
                     /translation="MSSVVRTRARGSRRIAGDWLLTLPLSVFFLAFVAVPLALLAAIS
                     LHNDAAMTHAGLHQYARFFSDSYNRGILWSTLGLGVWTTVSCIVLGYPVAWLYVSSTA
                     RWRRPLLLCILLPLLTSSVVRTFAWVVILGNEGLVNQVLAALGLIGSPIKLLYTPTAV
                     VLSLAQIELPLMVLPLATALSAIDPRFSEASHALGASPWRTWWRVTLPLSVPGLLSGT
                     LLVFTGAVSAFVVQTLVGGGQLMYMPFYIYQQAIQSQDYPFAAAIAMILLVSILVIVT
                     LLNYGGRKTKGFIHG"
     gene            complement(1397..2458)
                     /locus_tag="VHV99_10530"
     CDS             complement(1397..2458)
                     /locus_tag="VHV99_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX3635909.1"
                     /translation="MSKIDLDRRRIVQALGALGAVGLPLSGVIGKAYAAESLAATTFP
                     GAWEESHRKILLPAFRKATGQSATLTASQAVDTLTKLTGARANPPFDVVMMDEGPYLI
                     GVSQGLFEPMPASKMPNLADLPAKFTDPKGLGAYPSGQVYGIAYNTEKIKQAPTSWND
                     LLKPQFKGRVGLVGLDSTLGLVWMVALAKMLGGDEAHMDPAFDFIRRLMPNVGAVAAN
                     PGALGTLFQQGQIDISCHYINNVESLKAKGVPVALARPASNWGLVRSTMNIVKNTKMA
                     DLAAAYINTALSADVQQQMTAAPYFVAPTNKKVPFGGALAQIAQSSSELESFVQVDWA
                     KLNPLRAGYIDRFNREVKV"
     gene            complement(2490..3614)
                     /locus_tag="VHV99_10535"
     CDS             complement(2490..3614)
                     /locus_tag="VHV99_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3635910.1"
                     /translation="MNQYESVDVRFERIEHRFGDTSVIKDLDLKIDGGELVALLGPSG
                     CGKSTLLRILAGLQVQSAGRVLIGGDCVDGLAPRQRGVGIVFQNYALFPHMSVAANVA
                     YGLEANGATRRDALREAGAMLEMVRMDAFAARYPRELSGGQQQRVALARTLAVKPRIL
                     LLDEPFAALDKSLRLDMQIEIRRLQRELHITTIMVTHDQEEAMSMADRVAVLNRGCLE
                     QFAPATDIYDRPATPFVAGFVGTANLIAAQLSQQGDVMHAKVGDGVLVLERPDCDGMS
                     GARSEPALLAVRPEQWDWFPEADARPSSAFATIQLVLPLGAMLMVEAVLDGGIAVKLT
                     MPRSGPVALSPGTRVALRIKPHASVRVFGSALPDTALAAH"
     gene            complement(3611..4957)
                     /locus_tag="VHV99_10540"
     CDS             complement(3611..4957)
                     /locus_tag="VHV99_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3635911.1"
                     /translation="MHPVTYDLLITGATVLTADPAQPVLRDAAVAISGNRLAFVGRAA
                     DLPAGTMAHETRRLDGRVIAPGYVNVHTHAILSMVRGVAEDLGFAPAYTPGIPHGHDV
                     RPDEAIALARLGAMEAMLFGSTLINDSYVHADLTLGAMAELGLRVYACGRLHDVDFSG
                     VADGRWEHNTSIGERTLDDALRLADRWHGTPDGRTGVQLAVHAPDTCSDAFLELIARA
                     AHERDLGVTTHLAQSRTEVAQVLRRSGKTPAQLLDSVGLLDSRLVAAHCLFLDPADIA
                     RVGRAGITVAHIPKGNATGGTMAPTPALRASGARIALGTDNMHADMTEVMRWALAIAR
                     IQLGEVASDWQPADALAMATCNGAAAMGLGDRLGCLVEGWLADLVVFDFRRAHLTPHP
                     NPLGTLVHTGMGRDVEMVIVDGRIVVDDGLPTLCDAQQVIEAGNRAAQQLWARATA"
     gene            complement(4962..5756)
                     /locus_tag="VHV99_10545"
     CDS             complement(4962..5756)
                     /locus_tag="VHV99_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX3635912.1"
                     /translation="MPLSHANAPASVDPSVLLAVDRLHKKFGDKTILDAVSLDIRRGE
                     TAVLIGPSGSGKTTFLRCLNFLETPDGGTIHLGGERIGSNAGEPAVSEPTLARQRRRF
                     GFVFQRFNLFAHLSALDNVSCGPHRVLGLSRDAARQRAAAALEQVHLAAHANKRPSQL
                     SGGQQQRVAIARALAMQPEVLLFDEPTSALDPELVREVLDVMRDLASARMTMLVVTHE
                     MSFARQVAHRVVFMDGGNIVETGSPDQIFGAPREQRTRRFLQHLQA"
     gene            complement(5773..6420)
                     /locus_tag="VHV99_10550"
     CDS             complement(5773..6420)
                     /locus_tag="VHV99_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX3635913.1"
                     /translation="MDWTLIGMSMPVLAVGLLATLRICAAAIVLGIALGAVLGFGAMS
                     RHAPVRFFVRAYVDFIRGTPLLIQIFLVYFALPVIGVNFNEYWAGVTALTLNAGGFIC
                     EIMRGGLQSIDAGQTEAAKSIGMRHRQIVLHVLFPQAWRRILPPLTNELISLVKGSAM
                     LSAISVYELTHAGQEIIATYFSPFEIFLLIALYYYALISALAWLSRYLERRLPQY"
     gene            complement(6433..>7287)
                     /locus_tag="VHV99_10555"
     CDS             complement(6433..>7287)
                     /locus_tag="VHV99_10555"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX3635914.1"
                     /translation="MTSRMTRRQFVGRLSAASLLLSAALPRLASAAGLSLDDIKKAGV
                     LRIGCEATYPPFTFREGGVLTGYDVDLANAFCKALGVKPEFVDTVWAGVIPALYAKKF
                     DLVMSGLSYTAEREKKVAFSIPYVEASQAMLIRAADQASITGMREMNGKILGLKTGSP
                     GQTLEPKLDAALKAAGGAGFAQVKDFDDHPAAYMALSQGRVDGVLNTLPTLGLAMKTA
                     PGRYAIVKGIGADNWAGLAARPDDTALIAYLDQQLRTFRHDGTLDALQKKWFGLSMAL
                     PDAVPALS"
BASE COUNT         1444 a         2327 c         2340 g         1176 t
ORIGIN      
        1 tgtcgagcaa cgcaggatcc agttgcgcgt agctcgccga cggtgtgtaa aggatataag
       61 gcgcgcatag ggcgatgtgt cccgcgagca ggctgtagaa cgacaacccc gcgctctcca
      121 gattgaaaag tacgagaata cccagcccga gcgccagtga cgggacggtc agcggcgcca
      181 tgaaaaacga atccagtacg cgcgcggcca gactccggcg acgcgcgagc gcaacggcgg
      241 cgcctactgc aaggaccgtc gctatcgcgg ttgcaagcgc agcgagtttc agactgagcg
      301 tcagcgcatc gataatttcc ggcgaatcga acacgagcgc ctgataccag cgcagtgaga
      361 atcccggggg tggaaacttg agcgaatagc ccgatgtgaa cgaggtaacg agcacgatca
      421 gagtcggcag catgagcagc agtacgccca ccccgccgat cagcgacatg gtcacgcgaa
      481 acgacatgcc tgccgcgcga gcgccccgtg cctcggcaac gttagccatg aataaacccc
      541 ttggtcttgc gaccaccata gttaagcagc gtgactatca cgagaatgct gacaagcagg
      601 atcattgcaa tagcggctgc aaacggataa tcctgcgact ggatcgcctg ctgatagata
      661 tagaacggca tgtacatcaa ctgcccgcca ccgaccagcg tctggacgac gaacgcgctg
      721 accgctccgg tgaagaccag gagcgtgccg ctgagaagac cgggtacgga aaggggcaac
      781 gtcacccgcc accaggttcg ccacggactc gcacccagtg cgtgcgacgc ttcggaaaag
      841 cgcggatcaa tcgcgctcag cgcagttgcc aaaggcaaca ccatcagggg cagttcgatc
      901 tgcgcgagcg acagcaccac ggccgtcggc gtgtaaagca gcttgatcgg cgagccgatc
      961 aggccaagcg cggcgagcac ctgattcacg agcccctcgt tgccgagaat cacgacccac
     1021 gcgaaggtgc ggacgaccga gctcgtgagg agcggcaaca ggatgcaaag cagcaacggg
     1081 cgccgccagc gcgcagtgct gctcacatac agccacgcga ccggataccc cagcacgatg
     1141 cacgacaccg tcgtccagac gccgagtccc agcgtggacc agaggatgcc gcggttgtag
     1201 ctgtcgctga aaaaacgcgc gtattgatgc aggccggcat gcgtcattgc ggcgtcgttg
     1261 tgcaggctga tcgcggcgag caacgcgagc ggcaccgcga cgaatgccag gaagaacacg
     1321 ctgagcggca gtgtcagcag ccagtcgccg gcaatgcgcc tgctgccccg cgcccgcgtc
     1381 ctcacgacgg aactcatcag accttgacct cccgattaaa ccggtcgata tagccggcac
     1441 gcaacggatt aagttttgcc cagtcgacct ggacaaaact ttcgagctcg ctcgacgatt
     1501 gcgcgatctg ggcgagcgcc ccgccaaacg gcactttctt gttggtcggt gcaacgaaat
     1561 acggtgcggc ggtcatctgt tgttgaacgt cggcgcttag cgcggtgttg atatacgcgg
     1621 cggcaagatc ggccatcttc gtgtttttaa cgatgttcat ggtactgcgc acgaggcccc
     1681 agttgctggc gggtctggcc agcgcgacgg gcacgccctt cgccttcaac gattcgacgt
     1741 tgttgatgta gtggcacgat atatcgatct gcccttgctg gaacaaggtc ccgagcgcgc
     1801 ccggattggc cgcgacggcc ccgacgttcg gcatcagccg gcgaatgaaa tcgaacgccg
     1861 gatccatgtg cgcttcgtct ccgccgagca ttttcgcgag tgcgaccatc cagaccagac
     1921 cgagcgtgga atcgagtccg accagcccca cgcgcccctt gaattgcggc ttcaggaggt
     1981 cgttccacga cgtcggcgcc tgcttgatct tctccgtgtt gtacgcaatg ccgtagacct
     2041 gtcccgacgg atacgcgccc agacccttgg gatcggtgaa cttcgccggc aagtcggcga
     2101 gattgggcat cttcgatgca ggcatgggtt cgaacaaacc ttgcgaaacg ccgatgagat
     2161 agggaccctc gtccatcatc accacgtcaa acggcggatt cgcgcgcgcg cccgtcagtt
     2221 tcgtgagcgt gtcgacggcc tgggaggccg tgagcgtcgc gctctggccg gtcgccttgc
     2281 gaaaagcggg cagcaggatc ttgcgatgcg attcttccca agcgccggga aaggtggtcg
     2341 ccgccagcga ctccgcggca tacgctttgc cgataacccc gctaagcgga aggccgacag
     2401 cacctaaagc gcccagcgcc tggacaatcc tgcgtcggtc gagatcgatc ttggacatca
     2461 gaaactccgt gaaaggaaaa gaaatcgggt caatgggcgg ctaacgccgt gtcgggcaaa
     2521 gcactgccga agacacgcac cgacgcatgc ggtttgatcc gcaaggcgac gcgcgtgccg
     2581 gggctgagcg cgaccgggcc gctacgtggc atggtcagct tcacagcgat accgccatcc
     2641 agcacggctt ccaccatgag cattgcgcca agcggcagca cgagctggat ggtggcgaac
     2701 gcagaggaag gccgcgcatc ggcctcggga aaccagtccc actgttcagg acgcacggcc
     2761 agcagcgccg gctcggagcg cgcgccggac atgccgtcgc aatcgggccg ctcaagcacc
     2821 agcacaccgt cgcctacttt tgcgtgcatc acgtcgccct gctgtgaaag ctgggcggca
     2881 atcagattgg cggtgccgac gaagccggcc acgaacggcg tggccgggcg atcgtagatg
     2941 tcggtcgcgg gcgcgaactg ctcgagacat ccccggttga gcacggcgac gcggtcggcc
     3001 atgctcatcg cttcttcctg gtcatgcgtc accatgatgg tggtgatatg cagttcccgc
     3061 tgcaggcgcc ggatctcgat ctgcatgtcc agacgcaggc ttttgtcgag tgcggcgaag
     3121 ggttcatcga gtaacaggat gcgcggcttg accgccagcg tgcgcgccag cgccacccgc
     3181 tgctgctggc cgcccgacaa ctcgcgggga taacgcgcgg cgaaggcgtc catgcgcacc
     3241 atctcgagca tggcgccggc ttcgcgcagc gcatcgcgcc gcgttgcgcc attggcctcg
     3301 agcccgtagg ccacgttggc cgcaaccgac atgtgcggaa acagtgcgta gttttgaaac
     3361 acaatgccga ccccgcgctg ccggggcgca agcccgtcga cgcaatcgcc gccaatcagc
     3421 acgcgcccgg cgctctgcac ctgcagcccg gcaagaatgc gcagcagcgt gctcttgccg
     3481 cagccggacg gaccgaggag cgcaaccagt tcgccgccgt cgatcttcag gtcgaggtcc
     3541 ttgatgacgg atgtatcgcc gaaacgatgc tcgatccgct cgaaacgcac gtctacgctt
     3601 tcgtattgat tcatgccgtg gccctggccc agagttgctg tgccgcgcgg ttgccggctt
     3661 ctatgacttg ctgcgcatcg cagagcgtgg gaaggccgtc atccacgacg atacggccgt
     3721 ccacgatcac catctcgaca tcgcggccca tgccggtgtg cacgagcgtg ccgagcggat
     3781 tcgggtgcgg cgtcagatgc gcgcgacgaa agtcgaacac caccaggtcc gccagccagc
     3841 cttcaaccag gcaccccaga cgatcgccga gtcccattgc ggcggcgccg ttgcaggttg
     3901 ccatcgcgag tgcgtcggcg ggttgccagt cgctagcgac ttcgccgagc tggatgcgcg
     3961 caatggccag cgcccagcgc atgacttcag tcatgtcggc atgcatgttg tccgtgccaa
     4021 gcgcgatcct cgcaccgctc gcgcgcagag ccggtgtggg cgccatggtc ccgccggtcg
     4081 cgttgccctt ggggatatga gccaccgtga tgcccgcccg gccgacacgc gcgatatcgg
     4141 caggatcgag gaacaggcaa tgcgccgcga caaggcgcga gtccagcagc ccgacggaat
     4201 cgagcaattg cgcgggcgtc ttgcccgagc ggcgcaagac ctgcgcgact tccgtacggc
     4261 tttgtgcaag atgcgtggtg acgccaaggt cgcgctcatg cgctgccctc gcgatcagct
     4321 caagaaacgc gtctgaacag gtatccggcg catggaccgc cagttgcacg ccggtgcgtc
     4381 cgtcaggggt gccgtgccag cggtccgcaa ggcgcaaagc gtcgtcgagg gtgcgctctc
     4441 cgatcgacgt gttgtgctcc cagcggccat cggcgacgcc cgaaaagtcg acgtcgtgca
     4501 ggcggccgca ggcgtacacg cgcaagccca gctcggccat cgcgccgagc gtcaggtcag
     4561 cgtggacata actgtcgttg atcagggtcg agccgaacag catggcctcc atcgcaccga
     4621 ggcgcgcgag cgcaatcgct tcatcggggc gcacgtcgtg accgtgggga atgcccggcg
     4681 tgtaggcggg agcaaaaccg aggtcttccg cgacgcctct caccatgctg aggatcgcgt
     4741 gcgtgtgaac attgacataa cccggcgcga tgacacgtcc atcgagacgc cgggtctcgt
     4801 gcgccatggt gccagcgggc agatcggctg cccgtccgac gaacgcgagc cggttgcccg
     4861 agatagcaac ggcggcatcg cgcaggactg gctgcgccgg gtctgccgtc aggacggtcg
     4921 caccggtgat gagtaaatcg taagtaacag ggtgcatgag ttcaggcctg caaatgctgg
     4981 agaaagcggc gggtgcgctg ctcgcgcggc gcgccgaaaa tctggtccgg cgaaccggtt
     5041 tcaacgatgt tgccgccgtc catgaacaca acgcggtgcg cgacctgccg cgcgaacgac
     5101 atctcgtgcg tgacgaccag catcgtcatg cgagcgctgg cgaggtcgcg catgacatcg
     5161 agcacctcgc gcacgagttc ggggtcgagc gcggacgtgg gttcgtcgaa gagcagcacc
     5221 tcgggctgca tggcaagcgc gcgggcgatc gccacgcgtt gctgctgccc gcccgataac
     5281 tggctcgggc gcttattcgc gtgagccgcc agatgcacct gctcgagcgc ggcggcggcg
     5341 cgttgccggg ctgcgtcgcg tgacagtccg agcacccgat gcggtccgca agacacgttg
     5401 tcgagcgcgc tcagatgcgc gaacaggtta aaccgctgga acacgaagcc gaaccggcgg
     5461 cgttgccgcg cgagcgttgg ctcggacacg gccggctcgc ccgcattgct gccgatccgt
     5521 tcgccgccca gatggatcgt gccgccgtcc ggtgtttcga ggaaattgag gcagcgcaaa
     5581 aacgtggtct tgcccgaccc tgacggcccg atcaggaccg cggtctcgcc gcgccggatg
     5641 tcgagggata ccgcgtcgag aatcgtcttg tcgccgaatt tcttgtgcag ccggtccacc
     5701 gccagcaaca cggacgggtc aacggaagca ggcgcattag cgtgagataa aggcatgggg
     5761 tcttgaacca gatcagtact gcggcaagcg ccgctcgaga taacgcgaca gccacgcaag
     5821 cgccgagatc agcgcgtagt aataaagcgc gatcagcagg aatatctcaa agggagaaaa
     5881 gtacgtcgcg ataatctcct gacccgcatg cgtcaactcg tacacggaga tcgcggaaag
     5941 catcgccgag cccttgacca gcgaaatcag ctcgttggtg agcggcggca ggatccgtcg
     6001 ccaggcttgc ggaaacaaga catgcagcac gatctgccga tgccgcatgc cgatcgactt
     6061 cgccgcttcg gtctggccgg catcgatcga ctgcagaccg ccccgcatga tttcgcaaat
     6121 gaagccgccc gcattcagtg tcagtgcggt gacgccggcc cagtactcgt tgaagttcac
     6181 gccgatcacg ggcaacgcga agtacacgag gaagatctgg atcaacagcg gcgtgccgcg
     6241 gatgaagtcg acatacgccc gcacgaagaa tcgcaccggc gcatgacggc tcatcgcgcc
     6301 gaagccgagc acggcgccca gcgcgatgcc cagcacgata gcggccgcgc aaatcctgag
     6361 cgtcgccagc aggccgactg ccagcaccgg catcgacatg ccgatcagag tccagtccat
     6421 gtgcgccccg tgtcaggaaa gagccggcac ggcgtcgggc agcgccatcg acaggccgaa
     6481 ccacttcttc tgcagcgcgt cgagcgtgcc gtcgtgcctg aacgtgcgca gttgctggtc
     6541 gagataggcg atcagcgccg tgtcgtcggg gcgtgcggcg aggcccgccc agttgtccgc
     6601 gccgattccc ttgacgattg cgtacctgcc gggcgcggtc ttcatcgcga ggcccagcgt
     6661 tggaagcgtg ttgagcacgc catccacgcg accctgggag agcgccatgt aagcggccgg
     6721 atgatcgtcg aaatccttga cctgggcgaa gcccgcgcct cctgcggcct tcagggctgc
     6781 atcgagcttg ggctcgagcg tctggccggg cgagccggtc ttgaggccga ggatcttgcc
     6841 gttcatctcg cgcatgccgg tgatgctggc ctgatcggca gcacgtatca gcatggcctg
     6901 cgacgcctcc acgtagggaa tcgagaacgc gactttcttt tcccgctccg ccgtatagct
     6961 caggccgctc atcaccagat cgaatttctt cgcgtagagc gcagggatga cgccggccca
     7021 cacggtatcg acgaattcgg gcttgacacc cagcgccttg cagaaagcgt tggcaagatc
     7081 gacgtcataa ccggtcagca cgccgccttc ccgaaacgtg aaaggcggat acgtggcttc
     7141 gcagccgatc ctgagtacgc cggctttctt gatgtcgtcg agcgataaac ccgcagccga
     7201 ggcaaggcgc ggcaaggccg ccgacaacag cagcgaagcg gcgctcagcc ggccaacgaa
     7261 ctggcggcgc gtcatacgtg aagtcat
//
LOCUS       DATASH010000379         3453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6978454,
            whole genome shotgun sequence.
ACCESSION   DATASH010000379 DATASH010000000
VERSION     DATASH010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6978454"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1455
                     /locus_tag="VHV99_10560"
     CDS             <1..1455
                     /locus_tag="VHV99_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HEX3635915.1"
                     /translation="VVALVNIVGGLAVGIAQRGMSFADALHAYTILTVGDGLVSQIPS
                     LIVSVAAGILVTRVASADSADAHLGGDIYRQLAMHPKAIGMAGIACLSLAAIPNFPHI
                     QFLVAGGLLLALAVTLMRNAVLATNSQRALMPEMTRDGGNYVPRILDDVELGTSATLR
                     LRLGLDAFDALRPEALNRQLGQLRRNLMVDLGVPFPGLALLRDSRLAPERYLVDIEDV
                     PFASGTLVAAHTLVSGDENRIGFDGVEGYRPRAEKSVWVPNAKAAAIDDPEIRKASVD
                     EALCDHLAEICERSAADFLGTQETRFLLDQLAIEFRELVAQAGQAASTVQLAGVLRQL
                     LQQQVPVRNLRAILEAVVRVPAGERTLDRMVREARIQLGRQIARAYADLQSWTISAAV
                     LDPAWEASLEAQIRSGVDGEPQCVLSAEELTRAQQFLAADLSGIRLIVTNAVLRPHLV
                     RLLRDFGLRIEVLAIEEIPLDVFRIQTVATLGAP"
     gene            1466..2794
                     /locus_tag="VHV99_10565"
     CDS             1466..2794
                     /locus_tag="VHV99_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0046034 - ATP metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HEX3635916.1"
                     /translation="MRATESSAPMFMSAPSLRGRVVEARGVIVRVVGVSLRVGEKVRL
                     VRPDTLEAQYGEVVGFAHDGALVMPLAGLAGLSDITEVQGCGSAWGSVAATGLLGRVV
                     DGLGNPLDGGPPPPASATAAQDLPGDAAATLNPLERPVISVPFATGVRAIDGLLTCGV
                     GQRTGIFAPAGGGKSTIMGMIANGASTDAIVVALIGERGREVAEFIHDHLEHRRASTI
                     VIAATSDRPAAERIKAAQLASQVAVGLRATGRNVLLLFDSLTRYARALRELGLAVGEP
                     PLRGGFPPSVFAQLPRLIESAGVTAQGSITAFYTVLADEADLSDPVAEEARSLLDGHI
                     QLSSKLGASGHYPAIDILRSRSRLMNRVADTTQRADANRVRDWLARYEEVEMLLQIGE
                     YERGHDPETDRAIDQRPAIQRFLRQPYDRCSPWIETRAQLHGLCAGDRPA"
     gene            2791..3231
                     /locus_tag="VHV99_10570"
     CDS             2791..3231
                     /locus_tag="VHV99_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635917.1"
                     /translation="MMRGAAQEAQLWQLLARVRGLRVRRRLRALADARRRERQAAAAV
                     AEQVAVLERHAEERLHVLGFCRRDQRAGRQWHATLRAHDARLPDLQRQLSDAQHAHAA
                     ARHEAAEALRDWSVERVRHDDAKHRWRVALARVACAADGGRDGG"
     gene            complement(3274..>3453)
                     /locus_tag="VHV99_10575"
     CDS             complement(3274..>3453)
                     /locus_tag="VHV99_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635918.1"
                     /translation="DPNLPMKDRAAAAFGMVLNGVLAPAYMTGFDLNNYHFGRDQSAD
                     LRRAMQMFVQFDPHA"
BASE COUNT          525 a         1084 c         1251 g          593 t
ORIGIN      
        1 gtcgtcgcgc tggtgaatat cgtcggcggc ctcgcggtgg ggatcgcgca gcgcggcatg
       61 tcgttcgccg acgcgttgca tgcctacacg attctcacgg tcggcgatgg tctggtctcg
      121 cagatcccgt cgctgatcgt ctcggtggcg gccggtattc tcgtcacccg cgtggcctcg
      181 gccgacagtg ccgatgccca tctcggcggc gacatttacc ggcaactggc catgcatccc
      241 aaagcgatcg gcatggccgg gattgcctgt ctgtcgctgg cggcgatccc caattttccg
      301 cacatccagt ttctggtggc cggtggtctg ctgctcgcgc tcgcggtcac gctgatgcgc
      361 aacgccgtgc tcgcgacgaa ctcgcagcgc gcgctgatgc ccgagatgac gcgcgacggc
      421 ggcaactacg tgccgcgcat tctcgacgac gtcgagctcg gcaccagtgc gacgttgcgg
      481 ttgcggctcg gcctcgatgc cttcgatgcg ttgcggcccg aagcgctgaa ccggcagctc
      541 ggccaactgc gccgcaatct gatggtcgac ctgggcgtgc cgtttcctgg cctcgcgctg
      601 ctgcgtgact cgcggctcgc gccggagcgc tacctcgtgg atatcgagga cgtgccgttt
      661 gcgagcggca cgctggtggc cgcccacacg ctggtgagcg gcgacgaaaa ccgcatcggc
      721 ttcgacggtg tcgagggcta ccggccacgt gccgaaaaat cggtctgggt gccgaacgcg
      781 aaggcggccg cgatcgacga tccggagatc cgcaaggcga gcgtcgacga ggcgttatgc
      841 gatcacctgg cggaaatctg cgagcgttcg gcggccgatt ttctcggcac tcaggagacg
      901 cgcttcctgc tcgatcagct ggcgatcgaa tttcgcgagc tggtcgcgca ggcggggcag
      961 gccgcgagca cggtgcagct cgccggtgtg ttgcggcaat tgcttcagca gcaggtgccg
     1021 gttcgcaacc tgcgcgcgat cctcgaagcc gtggtgcgcg tgccggcggg cgagcgcacg
     1081 ctcgaccgga tggtgcgcga agcgcgtatc caattggggc ggcagatcgc acgcgcttac
     1141 gccgacctgc aaagctggac gatctcagcg gccgtgctgg acccggcatg ggaggccagc
     1201 ctcgaggcgc aaattcgcag cggcgtggac ggcgagccgc agtgcgtgct gtcggcggaa
     1261 gagctgactc gcgcacagca gtttctggcc gcggatctgt cggggatccg cctgatcgtc
     1321 acgaacgcgg tgctgcgtcc gcatctggtg cggttactgc gcgacttcgg attgcgcatc
     1381 gaagtgctgg cgatcgaaga gattccactc gacgtcttcc gtatccagac cgtcgcgacg
     1441 cttggcgcgc cataaggagg aactcatgcg agcgacggaa agcagtgcgc ccatgttcat
     1501 gtccgcgccg agcttgcgcg ggcgggtggt cgaagcgcgc ggtgtgatcg tgcgggtggt
     1561 gggtgtttcg ttgcgggtcg gcgagaaagt gcgcctcgtg cggccggata cgctcgaagc
     1621 gcagtacggt gaagtggtcg gctttgccca cgacggtgcg ctggtgatgc cgctggcggg
     1681 tctggccggc ctgtcggata tcacggaggt gcagggctgc ggctcggcct ggggcagcgt
     1741 cgcagcgact gggttgctgg gacgcgtggt ggacgggctc ggcaatccac tcgacggcgg
     1801 tccgccgccg ccggcctctg caactgctgc gcaagacctg ccgggcgatg ccgcggcgac
     1861 gctcaatccg ctcgaacggc ccgtcatcag tgtgccgttt gccacgggtg tgcgtgcgat
     1921 cgacggcctg ctgacctgtg gcgtcggtca gcgcacaggc atcttcgcgc cggcgggcgg
     1981 cggcaaaagc acgatcatgg gcatgatcgc gaacggcgcg tcgaccgatg cgatcgtggt
     2041 tgcactgatc ggtgaacgcg gccgcgaggt ggccgaattc attcacgatc atctggaaca
     2101 ccggcgcgcg tcgaccatcg tgatcgccgc gacgtccgac cgtcccgcgg cggagcgcat
     2161 caaggcagcg caactggcgt cgcaggtagc ggtgggtttg cgcgcgaccg gacgcaacgt
     2221 cctgctgttg ttcgactcgc tgacgcgcta tgcgcgcgcg ctgcgcgagc tcggcctcgc
     2281 agtcggcgag ccgccgctgc gcggtggctt tccgccgagt gtgtttgcgc aactgccgcg
     2341 cctgatcgaa tccgccggtg tgaccgcgca gggcagcatc accgcgtttt ataccgtgct
     2401 tgcggatgag gccgatttgt cggatccggt ggcggaagaa gcgcgctcgt tgctcgatgg
     2461 gcatatccag ttgtcatcga aactcggcgc gtcgggccac tatccggcga tcgacatctt
     2521 gcgcagtcgc agccgcttga tgaatcgcgt cgcggatacc acgcaacgcg ccgacgcgaa
     2581 ccgtgtgcgc gactggctgg cgcgttatga ggaggtcgag atgctgttgc agatcggcga
     2641 atacgagcgg ggtcacgatc ccgagacgga ccgggcgatc gatcagcgcc cggcgatcca
     2701 gcgctttcta cgtcaaccgt acgaccggtg cagcccgtgg atcgaaacgc gcgcgcagct
     2761 acatggcttg tgcgccggcg atcgcccggc atgatgcgcg gcgccgcgca ggaggcgcag
     2821 ctctggcagc tgctcgcgcg agtgcgcggt ttgcgggtgc gccgccggct gcgtgcactg
     2881 gctgacgcgc gccggcgcga acggcaggcg gcggccgcgg tggccgaaca ggttgccgtg
     2941 ctggaacggc acgccgaaga acggctgcat gtgctcgggt tctgccgtcg cgaccagcgc
     3001 gccggcagac agtggcacgc gacgctgcgc gcgcacgacg cacgccttcc cgatttgcag
     3061 cggcaactga gcgatgcgca gcacgcgcac gcggcggcgc gccacgaagc ggctgaggcg
     3121 ctgcgggact ggagcgtcga gcgggttcgg catgatgacg caaaacatcg ctggcgggtg
     3181 gcgctcgcgc gcgtggcatg cgcggcggat ggtggacggg acgggggtta gcgccgccta
     3241 aatgggggcg ctgggccgcc cagtcaacgc tagtcaggca tgaggatcaa actgcacgaa
     3301 catctgcatc gcccggcgca gatcggccga ttgatcgcga ccgaagtggt agttgttcag
     3361 gtcgaacccg gtcatgtaag ccggcgcaag cacgccgttc aagaccatgc cgaacgctgc
     3421 ggcagcgcgg tctttcatcg gcaggttcgg gtc
//
LOCUS       DATASH010000380         2850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_55206,
            whole genome shotgun sequence.
ACCESSION   DATASH010000380 DATASH010000000
VERSION     DATASH010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55206"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            540..1048
                     /locus_tag="VHV99_10580"
                     /pseudo
     CDS             540..1048
                     /locus_tag="VHV99_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006052.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase"
     gene            1045..2646
                     /locus_tag="VHV99_10585"
     CDS             1045..2646
                     /locus_tag="VHV99_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEX3635919.1"
                     /translation="MSFAKEFRTRSFDDVRRTLLDREEIALVDVREEDPHARSHPLFA
                     ANLSLSRLELEAPVRLPRRDVPIVVFDNGEGLAERAALRLSELGYTDVALLEGGLQGW
                     REAGGELFQDVNVPSKAFGELVESVRHTPSLAAPQVLSMLDHEADVVVLDARRFDEYQ
                     TMNIPGSVSVPGAELVLRARQLAPNPATRIIVNCAGRTRSIIGAQSLINAGVPNPVAA
                     LRNGTIGWTLAGQALAHGASSRFEPLVDDALRLAAADSARGVADRARVGRTSRDEARR
                     WADEAVRTVYRFDVRTPEEYEAGHVPGFRNAPGGQLVQETDMFAPVRGARVILADNDG
                     VRANMTASWLAQMNVEVYVVDGLGNADFGEKGPAPAARHAPAPPDVDEIAPAGLVTLL
                     QSRGTVVLDFTTSANYVKRHIPGAWFVIRGQLDEALRKLPDAQRYVLTCGSSVQTRFA
                     APEVAALTGKPVQVLSGGTNAWIEAGLAVESGETRLASPRIDRYRRPYEGTDNAREAM
                     NAYLEWEYGLVAQLGRDGTHGFRVL"
BASE COUNT          572 a          837 c          929 g          512 t
ORIGIN      
        1 gggcgcatcg ccgagacaag gccgatggct cccacctgct cccaaacgaa gccgctggtt
       61 tgcatgatga acccttctgg cgagcacgca gggcgaggcg ggaagtatga cagccttgtc
      121 actcgcgccg aatgtgcgag aacacggatt ccagatgcac cactaccgcg actgtgcggc
      181 ggacccgctt aagaatttgc ggtttgagcc gctttctttt gcctactttt ctttgcggca
      241 ggcaaagaaa agtaggtgcc tgccccgcac aggggcgacg ctaatagacc aatatcaatt
      301 caaggaaagg ccaacgccgt aagcaaacag acaaccacgc gcgagcagac aaaaaaatca
      361 aggaaaggcc accgccgcag gcaaacagac aaccacgcgc gaacagacaa acaaaacagg
      421 aaaaggccaa cgccgcaggc atacaaccaa gcgctgcgca gcgaaaaccc ctcctcatta
      481 catgcaaacc cgacatagat atgtccgatc aatatttaac aaccgcatgt ctggaagccg
      541 gcggtgccgc gccacgtgaa ctgatcgccc acgacgactg attgcccgac gccttcacgc
      601 aacccgatcc cgagcgctat cagcaattcc tgccgcacgc cgattcgcga cagcgctttt
      661 caatcgtcag ttttgtatgg ggaccggggc agtcgacgcc ggtgcacgat cccaccgtat
      721 ggggtttgat cggcgtgttg cgtggtgcgg aaattgctca cggatatcac atcacgtcag
      781 acggaatact ggaggaagag gggcggcaaa acgcctcggt gccggcacag tggacgccgt
      841 gtcgccgcgc aaaggcgata tccatcgcgt gagcaatgct tttggtgatc gtacgtcgat
      901 cagcattcac gtgtacggtg gaaatatcgg tgcggtgacg cgttccgtct atcccgtcag
      961 cggaggccgc aaaactttta tttccggtta ctcgaacgac gtgttgccga atatctggaa
     1021 tgtttcaaag gagtccccga tctcatgagt ttcgctaaag aattccgtac ccgatcattc
     1081 gacgatgtgc gccgcacctt gctggatcgc gaggaaatcg cgctcgtcga cgtgcgcgag
     1141 gaggatccgc acgcgcgcag tcacccgctg ttcgctgcga atctgtcgtt gtcgcggctc
     1201 gaactggaag cgccggtgcg cttgccgcgt cgcgacgtgc cgattgtcgt gttcgacaac
     1261 ggcgaagggc ttgccgaacg cgccgcgctc cggttgagcg agttgggtta taccgatgtc
     1321 gcgctgctgg agggcggttt gcaaggctgg cgcgaggcgg gcggcgagct gttccaggac
     1381 gtcaacgtgc cgagcaaggc attcggcgaa ctggtcgaaa gcgtgcgcca tacgccctcg
     1441 ctggcagcgc cgcaggtgct gtcgatgctc gatcatgaag ccgacgtggt cgtgctcgat
     1501 gcacgccgct ttgacgaata ccagaccatg aatattcccg gcagcgtgag cgtgcccggc
     1561 gcggagcttg tgttgcgcgc gcggcagctc gcgccgaatc ctgccacacg gatcatcgtc
     1621 aattgcgcgg ggcgtacgcg cagcatcatc ggcgcgcagt cgctgatcaa tgcgggcgtg
     1681 ccgaatccgg ttgcggcgct gcgcaatggc acgatcggct ggacgcttgc gggccaggcg
     1741 ctcgcgcacg gcgcctccag ccgcttcgag ccgcttgtcg atgacgcact tcgcctcgcc
     1801 gcggccgatt ccgcgcgcgg cgtggcggat cgtgcgaggg tgggccgcac ctcgcgcgac
     1861 gaagcgcgcc gctgggccga cgaagcggta cgcaccgtgt atcgcttcga cgtgcgcacg
     1921 cccgaagaat acgaagcggg gcacgtgccc ggctttcgca acgcgcctgg cggtcaactg
     1981 gtgcaggaga ccgacatgtt cgcgccagtg cgcggcgctc gcgtgatcct tgccgacaat
     2041 gacggcgtgc gcgcgaacat gactgcgtca tggctcgcgc agatgaatgt cgaggtgtat
     2101 gtcgtcgatg gcttgggcaa cgcggacttc ggcgaaaaag gccccgcgcc cgccgcgcgc
     2161 catgcgcccg caccgcccga tgtcgatgag atcgcgccgg caggactggt tacgctgttg
     2221 caatcacgtg gcaccgtcgt gctggatttc acgaccagtg cgaactatgt gaagcgccat
     2281 attcccggcg cgtggttcgt gattcgcgga cagctcgacg aggcattgcg caagctgccg
     2341 gatgcgcaac gttatgtgct cacctgtggc agcagtgttc agacgcgttt cgccgcgcct
     2401 gaggtggcgg cgttgacggg taagccggtg caggtgctaa gcggcggcac gaacgcgtgg
     2461 atcgaagcgg gtctcgccgt ggaaagcggc gagacgcgtc tggcgtcgcc gcgcattgat
     2521 cgttatcgtc gtccgtatga gggaaccgat aacgcgcgcg aagcgatgaa tgcgtatctg
     2581 gagtgggagt atggcctcgt tgcgcaactt gggcgcgatg gcacgcatgg atttcgcgtg
     2641 ctctgacggg ccttcctgag tgaagcagac gaggttggtg aatcaggtca ggctgatgaa
     2701 atcaggtaag gccggtgagt cggccgacgc aggtgaagcg aaatcacgcg aggccggtga
     2761 gtcggtcgaa gcagctggag cgaaagcagg tgaaggcgct tgcagatcag ggtaggccac
     2821 cttgaggccg cgcatcgcat ccgtgagcgc
//
LOCUS       DATASH010000381         4198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3022374,
            whole genome shotgun sequence.
ACCESSION   DATASH010000381 DATASH010000000
VERSION     DATASH010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3022374"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="VHV99_10590"
     CDS             complement(<1..469)
                     /locus_tag="VHV99_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEX3635920.1"
                     /translation="MTRPLLQRALPKTLLARNIALLIVLVMLSQICSLGVLLHYVQRP
                     RVERAATVFATYVTTLDSLLQATPADARAGLIARLNGRAELPREATLEAPRSVLRAYR
                     SYQRNVFVESLRAHLPADMPARWQTEGGERLWIRLHAPADAPQAPYWIALPIPE"
     gene            complement(466..1179)
                     /locus_tag="VHV99_10595"
     CDS             complement(466..1179)
                     /locus_tag="VHV99_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3635921.1"
                     /translation="MDRPAKIIVLDDEAELRNMLQRFLRSHGFEVRIAEDGKRLDRYL
                     EREPFDLLVLDLMIGEEDGLEICARLRAQGQTLPILMLTAKGDPLDKVAGLETGADDY
                     LAKPFLPRELVARINALLRRQKIASGDVTVTSQSVRFGDFSLDVGKQQLSRAGELLEL
                     HSAQMLLLVALASSPNRPVSRDNLLARARGREHDALDRSIDVQVLRLRQIVEDDPSKP
                     RFIKTVWGVGYMLIADVDS"
     gene            complement(1315..1608)
                     /locus_tag="VHV99_10600"
     CDS             complement(1315..1608)
                     /locus_tag="VHV99_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HEX3635922.1"
                     /translation="MKKLIAVVLLCCASTVALAQTAAPQGGNPQRMEHMAQQLQTRFA
                     NANSTHDGKLTREQAAAGMPMVAKHFDEIDTQKAGYITLPQIAAFMQERGAAH"
     gene            2106..2351
                     /locus_tag="VHV99_10605"
     CDS             2106..2351
                     /locus_tag="VHV99_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635923.1"
                     /translation="MSYQDIDKEIAHLELVFGQISVNDRIPLSYWRNRLHKLPNTSLM
                     PTQRARLARLEAALRSLEQSAQKVASEPPMRSTGTRS"
     gene            complement(2379..3629)
                     /locus_tag="VHV99_10610"
     CDS             complement(2379..3629)
                     /locus_tag="VHV99_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic transporter"
                     /protein_id="HEX3635924.1"
                     /translation="MNSVFLSWGIAIAATAGVITRPFKWPEAVWAVAGALLLVSLGLL
                     PVDQAIEAVGKGTDVYLFLFGMMVLSEVGRREGLFDWVAVFAVNHAKGSPGKLFLLVY
                     LVGVVITAFLSNDATAVVLTPAVFAAAKKAKTHPLPLLFVCAFIANAASFVLPISNPA
                     NIVLYGNHTPALGAWLMRFTLPSLLSIVATYVLLRWTQREALAGTCESDLEPLELSSS
                     GRVALAGIAVTAVVLLTVSAFDVPLGLPTAILGALTAAVVLIQERKSPLPMIQEISWS
                     VLPLVAALFVLVEMLDHTGVIKTLVDLVQGATQHSELATSGWAGAMIAIGSNLMNNLP
                     AGLIASSTVMQAHSPERVIDALLIGVDLGPNLSITGSLATILWLNAIRREGEDVSFMK
                     FLKVGAVVMLPALALALAARILIG"
     gene            4013..>4198
                     /locus_tag="VHV99_10615"
     CDS             4013..>4198
                     /locus_tag="VHV99_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEX3635925.1"
                     /translation="MKPRPSDIKHYSGPAIALHWLIALLIVCGFYLGWIMTDIPGFTP
                     TKLKYFSWHKWIGVTVFV"
BASE COUNT          854 a         1308 c         1255 g          781 t
ORIGIN      
        1 cctcgggaat cggcaacgcg atccagtacg gcgcctgcgg tgcgtcggcc ggcgcgtgca
       61 ggcgaatcca cagacgctcg ccgccttcgg tttgccagcg cgccggcatg tcggcgggca
      121 gatgcgcgcg caagctctcg acaaacacgt tgcgctgata gctgcgatag gcgcgtaaca
      181 cactgcgtgg cgcttcgagg gtggcctcgc gaggcagctc cgcgcgtccg ttcagacgtg
      241 cgatcaagcc ggcgcgcgca tcagccgggg tggcctgaag caggctgtcg agcgtcgtca
      301 cgtaggtggc gaacaccgtg gccgcccgtt cgactcgagg ccgctggaca tagtgcagca
      361 acacgccgag cgagcatatc tggctcagca tcaccagcac gatcagcaga gcgatattgc
      421 gcgccaacag cgttttcggc agcgcccttt gcagcagcgg gcgcgtcatg agtcgacgtc
      481 cgcgatcagc atgtagccga cgccccacac ggtcttgatg aagcgcggct tcgacgggtc
      541 gtcctcgacg atctgcctga gccgcaacac ctgcacgtcg atgctgcgat cgagcgcatc
      601 gtgctcacgg ccacgggcgc gcgccagcag attgtcccgg ctcacaggcc ggttcggcga
      661 cgaagccagt gcgaccagca acagcatctg cgccgaatga agctcgagca attctccggc
      721 acgcgacaac tgctgcttgc cgacgtcgag actgaaatcg ccgaagcgca cgctctgcga
      781 ggtcacggtg acatcgcccg aggcgatctt ctggcgacgc agcagcgcat tgatgcgcgc
      841 caccagctca cgcggcagga acggtttggc gaggtaatcg tcagcgccgg tttcgaggcc
      901 ggccaccttg tcgagcggat cgcccttcgc ggtcagcatc aggatcggca gcgtctgccc
      961 ctgggcacgc aagcgggcgc agatttcgag gccgtcctct tcgccgatca tcagatcgag
     1021 caccagcaga tcgaacggct cgcgctccag ataacggtcg agccgcttgc cgtcctccgc
     1081 gatccgcacc tcgaatccat ggctgcgcag gaaacgctgc agcatgttgc gcagctcggc
     1141 ttcgtcgtcg agcacgatga ttttggcggg gcgatccata cgaaagctcc tcggtttaag
     1201 cgtcgggcgg tgcatgccgc gccaggtgcc gatcggcctg gcgctgcgac ggggcaggtt
     1261 tcatcgcgcg ttgggacgcc aaagaactta cgatcccgcc gatgtccgcg ctactcagtg
     1321 cgctgccccg cgttcctgca tgaatgccgc gatctgcggc aacgtgatgt agcccgcttt
     1381 ttgcgtatcg atctcgtcaa aatgcttagc gaccatcggc atcccggcgg ccgcctgttc
     1441 ccgggtgagc ttgccgtcgt gcgtgctgtt ggcattcgca aagcgcgttt gtaattgttg
     1501 cgccatgtgt tccatgcgct ggggattgcc gccctgtggt gcggctgttt gagcgagcgc
     1561 gacggtggat gcacagcaca gcagcacaac ggcgatcaac ttcttcatga ctgactcctt
     1621 cgattgagcc atcgtgcgtc gcctgctaaa ccgccgcgat ccgttcgatg acaggccgaa
     1681 ttcttgggca agtgccggcg gaaggcaatt tcagaatcat ttcgccatat gtcatcggtt
     1741 tttggtggcc ttggcaaggc ggtcacgtac gttctcgtgc cggtatcctc gaaaataatc
     1801 accgctgtac acaacgcgcg acaggctcgc ttttatttag ggttccacga gatattgcgg
     1861 gaattaaaac aggtggaaga cgggtctcct cgacaggcca ccaggagtgc acggcgccac
     1921 acgtcagaca aatgaatgta gcttgaaagc tctgcgttag attaacatcg cggagggccg
     1981 gtacggcgct tgctacattc tgcatcacca tggggcgcgt ccattagtta gacatatctg
     2041 ttcaactggc ggcattccgc ttgcactgtt tcattgccga tccatctctt cttctggaat
     2101 gtcagatgag ctaccaagat atagacaaag aaattgccca tctcgaactg gtattcgggc
     2161 aaatttcggt caacgatcgc attccgctgt cgtattggcg caatcgtctg cacaagctcc
     2221 cgaacacctc actgatgccg acgcagcggg cacgcctcgc gcggctcgaa gccgcgttgc
     2281 gctcgctcga acagtccgcg caaaaagtgg cgtccgaacc gcccatgcgt tctaccggca
     2341 cccggtcatg acgcagggcg gatggagaaa ccatccgctc aaccgatcaa aatccgcgcg
     2401 gcgagcgcca gcgcgagggc cggcagcatg acgaccgccc ccactttcag aaacttcatg
     2461 aagctaacgt cttcgccttc gcggcgaatc gcgttgagcc aaaggatcgt tgcgagcgag
     2521 ccggtaatcg acagattcgg cccgaggtcg acgccgatca gcagtgcatc gatcacacgc
     2581 tcaggactat gcgcctgcat cacggtcgaa ctggcgatca gacccgccgg cagattgttc
     2641 atcagattgc tgccgatcgc gatcatggcg ccggcccagc cagatgtcgc gagttcactg
     2701 tgctgcgtcg cgccttgcac aagatcgacg agcgtcttga tgacgccggt gtgatcgagc
     2761 atttcgacca gcacgaacaa cgcggcgacg agcggcaata cgctccacga tatttcctgg
     2821 atcatgggca gcggcgattt gcgctcctgg atcagcacga ccgcggcggt cagcgcaccg
     2881 agtatcgcgg tgggcaggcc gagcgggaca tcgaaggcgg agaccgtgag caacaccacg
     2941 gccgtcaccg caatgcccgc gagcgcgacg cgcccgctcg acgacaattc gagcggctca
     3001 agatcggact cgcaggtgcc ggcgagcgct tcgcgctggg tccagcgcaa cagcacatag
     3061 gtcgccacga tcgacagcag cgaaggcagc gtgaagcgca tcagccaggc gccgagcgcg
     3121 ggcgtgtgat tgccgtacaa cacgatgttg gccggattcg agatcggcag cacgaagctc
     3181 gcggcattcg cgatgaacgc gcagacgaac agcaagggca acggatgcgt cttcgctttt
     3241 ttggcggccg caaaaacagc cggcgtaagc acaactgcgg tggcgtcatt ggaaaggaag
     3301 gcggtgatca cgacgccgac cagatagacc agcaaaaaga gctttcccgg cgagcctttg
     3361 gcgtgattga ccgcgaatac ggcgacccag tcgaataggc cttcgcggcg cccgacttcc
     3421 gatagcacca tcatcccgaa cagaaacaga tagacatccg tgcctttgcc gactgcctcg
     3481 attgcctgat cgacgggaag caggcccagc gacacgagta atagcgcgcc tgcaacggcc
     3541 cacacggcct cgggccattt gaacgggcgg gtgatgacgc cggccgtggc ggcgatggcg
     3601 ataccccagg agaggaagac ggaattcaac aagtaagtcc ctggattctg attgcgcgcg
     3661 aacgcgggat tgggcgcagc ataacccatg accggcatgc cgcaggcggt cgggacgcaa
     3721 attgtgtgcc gcgcgatacc gccttccctg cccggctacc gcacccggtt gattgcagca
     3781 atgcaatccc gcccattatc gttgacgtga tacgactgcg tatccaccag gttaatgaag
     3841 tccaatggga tcgccggcct caggcgtctt tattccatcc ccattagcgt cacggctaga
     3901 acgatctgtt caattatcct cggctagtca attttcatcg gtctccctac tatccgcatc
     3961 tacacgcgac ataccgcatg cggcaagcac cacgaccagt gagcctgaat caatgaaacc
     4021 ccgtccttca gacatcaagc attacagcgg ccccgcaatc gccctgcatt ggctaatcgc
     4081 attgctgatc gtctgcgggt tctatctcgg ctggatcatg accgatatcc ccggcttcac
     4141 gcccaccaag ctgaagtact tctcgtggca caaatggatc ggcgtgacgg tctttgtg
//
LOCUS       DATASH010000382         2656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1243996,
            whole genome shotgun sequence.
ACCESSION   DATASH010000382 DATASH010000000
VERSION     DATASH010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243996"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..1628)
                     /locus_tag="VHV99_10620"
     CDS             complement(72..1628)
                     /locus_tag="VHV99_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015600610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3635926.1"
                     /translation="MSATSSRVPLFGVEKLRGDFLPWVLAIVTGLDYFDTSIFSFFTS
                     HIAGGVNASPDELVWASSAYAVAAVLGILQQQWWVERFGYRRYVAGCMLLYSAGAIAA
                     TLCESSIELAFARGFQGYFIGPMMGTCRILIQMSFTPQQRPAATRAFLVLIVLSSALA
                     PLIGAQLVAHFDWRALFACTAPAGVLFAILALLALPETGNRLPEERGAAHFWPYLIFA
                     FAQGALQIVMQQVRFQLFSASPGLILLTVAGIVALGWFAYQQWHHPAPLVRLQALREK
                     VFQVGLVLYMFYYYISTVFSYLTSRFLEGGLGYPVENTGQLVGVTSLISASALFIYLR
                     YAKLLPRKKWIIVPGFAVAAFAAAWMSRMSPGVGEAALIAPMLLRGLLLLFIVLPVAN
                     LTFRIFAIEEFTHGYRLKNIVRQLTISFATASVIIVEQHRQALHQARLAEFANPYNPM
                     FQNSLAALTSGFSTAGRSPSEAHSLAIVEISRTVAQQASFLTSLDGFTFLLGVAICGG
                     IFAAWQKQID"
     gene            complement(1625..2146)
                     /locus_tag="VHV99_10625"
     CDS             complement(1625..2146)
                     /locus_tag="VHV99_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX3635927.1"
                     /translation="MSKDSPALLTVRDAAERLGVTPRTLKYYEERGLVSPTRSEGRYR
                     LYDEEDLKRFGRILRLRSLGFSLQGITEMLKRPLEPAEGGHRYSTESLQQIRDAIAAQ
                     VEALDARIETMRRELKEAQKLRAELSPDLDYLERRLAGENADALLEQRRNALAKAEST
                     PFAKRAKKPGEPT"
     gene            complement(2339..>2656)
                     /gene="rho"
                     /locus_tag="VHV99_10630"
     CDS             complement(2339..>2656)
                     /gene="rho"
                     /locus_tag="VHV99_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921615.1"
                     /note="An RNA-DNA helicase that actively releases nascent
                     mRNAs from paused transcription complexes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX3635928.1"
                     /translation="TALIETGSRMDDVIYEEFKGTGNMEVHLERRLAEKRVYPSINLN
                     KSGTRREELLIKPEVLQKIWVLRKFIHDMDEVESMEFLLDKIRQTKSNSEFFDMMRRG
                     GGS"
BASE COUNT          528 a          782 c          851 g          495 t
ORIGIN      
        1 cgcagcgcgc ggtcgcgtcg gcgcgtgccg aaccattgag tgagtttcga gagcatggcc
       61 tgatgaaacc cttagtcgat ctgtttttgc cacgcggcga agatgccgcc gcatattgca
      121 acgccgagca ggaaggtgaa accatccagc gaggtcagga agcttgcctg ctgcgcgacc
      181 gtgcggctga tttccactat cgcaagcgaa tgcgcctcgg aaggcgagcg gccggcggtg
      241 ctgaagccgc tcgtcagcgc cgcgagcgaa ttctggaaca tcggattgta cggattcgcg
      301 aactccgcga ggcgtgcttg atgcagcgcc tgccgatgct gttccacgat gatcaccgag
      361 gccgtcgcga acgagatcgt cagttgccgg acgatgtttt tcagccggta gccgtgcgtg
      421 aattcctcga tggcgaagat tctaaacgtc agattggcga ccggcagcac gatgaacagc
      481 aacagcaagc cgcgcagcag catcggcgcg atcagcgcgg cttcgcccac acccggcgac
      541 atgcgcgaca tccacgccgc ggcgaacgcg gccaccgcga agcctggcac gatgatccac
      601 ttcttgcgcg gcaaaagctt tgcgtaacgc aggtagatga acagcgcgct cgccgaaatc
      661 agtgacgtca caccgaccag ttgcccggta ttctcgaccg gatagccgag gccgccctcg
      721 agaaagcgcg acgtcagata gctgaatacg gtcgagatgt agtagtagaa catgtacagc
      781 acgagtccga cctgaaagac tttttcacgc agcgcctgca ggcgcaccag cggcgccgga
      841 tgatgccact gctggtacgc gaaccagccg agcgcgacga tgccggcgac cgtcagcaga
      901 atcagccctg gcgatgcgct gaacagctga aaccgcactt gttgcatcac gatctgcagc
      961 gcgccttgcg cgaacgcaaa gatcagataa ggccagaagt gcgccgcgcc gcgttcttcc
     1021 ggcaggcggt tgcctgtctc gggcagcgca agcagcgcaa ggatcgcaaa cagcacaccg
     1081 gcgggcgcgg tgcaggcgaa cagcgcacgc cagtcgaaat gcgcgaccag ttgagcgccg
     1141 atcagcggcg caagcgcact gcttagaacg atcagcacca ggaaagcgcg cgttgccgcg
     1201 ggccgttgct gcggcgtgaa gctcatctgg atcaggatgc ggcaggtgcc catcatgggg
     1261 ccgatgaagt agccctgaaa gccgcgcgcg aatgcgagtt cgatcgatga ctcgcacaat
     1321 gtggcggcga tcgcccctgc tgaatagagc agcatgcagc cggccacgta gcgccggtaa
     1381 ccgaagcgct cgacccacca ttgctgctgc aggatgccga gtacggccgc gaccgcatag
     1441 gcgctcgacg cccacaccag ttcgtcgggc gacgcgttga cgccgcccgc gatatggctg
     1501 gtgaaaaacg agaagatcga ggtgtcgaaa tagtcgaggc cggtgacgat ggccagcacc
     1561 cagggcaaaa aatcgccgcg caatttctcg acgccgaaca atggaacgcg cgatgaggtt
     1621 gcgctcatgt gggctcgccc ggctttttcg cgcgtttcgc gaacggtgtg ctctcagctt
     1681 tggcgagtgc gttgcggcgc tgctcgagca gtgcgtccgc attctcgccg gcgaggcgcc
     1741 gttcgagata gtcgagatcg ggactgagtt cggctcgcag tttctgcgct tccttgagtt
     1801 cgcgacgcat cgtttcgatg cgggcatcga gtgcttcgac ctgggctgcg atagcgtcgc
     1861 gaatctgctg cagcgattcg gtggagtagc gatgcccgcc ttcggccggt tcgagcggac
     1921 gcttgagcat ttcggtgatg ccttgcagcg agaagccgag cgaacgcagg cgcagaatgc
     1981 ggccgaagcg cttcaggtct tcctcgtcgt aaaggcggta gcggccttcg ctgcgggtag
     2041 gcgagacgag gccgcgctcc tcgtaatact tcagtgtgcg cggcgtgacg ccgaggcgtt
     2101 cggcggcgtc gcgtacggtg agcagggcgg gcgagtcctt cgacataagg tgtgtgtaga
     2161 catggcggac caagcccatt gtactgcaac gtgtacgttc acgtacatgt tatcgggata
     2221 gtcgtctcct tccgacgcaa aacctcacgg acgaaaaaaa accgctcgtt tctggcgagc
     2281 ggctttctta tgtgatgcgg acggtgcaga tagggtgctg cggtttcggc gcgaggtctt
     2341 aactgccgcc gccacggcgc atcatgtcga agaactcgga gttgctcttc gtctggcgaa
     2401 tcttgtcgag caggaattcc atcgactcga cttcgtccat gtcgtgaatg aacttgcgca
     2461 gcacccagat cttttgcagg acttcgggct tgatcagcag ttcttcgcgg cgcgtgcccg
     2521 acttgttcag gttgatcgac ggatagacgc gcttttcagc aaggcggcgt tccaggtgca
     2581 cttccatgtt gccggtaccc ttgaactctt cgtagatcac gtcgtccatg cggctgccgg
     2641 tttcgatcag cgcagt
//
LOCUS       DATASH010000383         3498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_848623,
            whole genome shotgun sequence.
ACCESSION   DATASH010000383 DATASH010000000
VERSION     DATASH010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848623"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VHV99_10635"
     CDS             <1..206
                     /locus_tag="VHV99_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635929.1"
                     /translation="ALKRCCKSGPTCNFSFNRLSDMRARPGAFRAELSFDCADFISFV
                     REKPQAPTWIKPDTHSHIQHVSA"
     gene            234..1154
                     /locus_tag="VHV99_10640"
     CDS             234..1154
                     /locus_tag="VHV99_10640"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011547925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX3635930.1"
                     /translation="MSRNAIQWSGVFPAVSTQFKPDFSLDVDATHRVVSNLVKDGVSG
                     LVVCGTVGENTSLSTAEKMAVIEAARDAAGGKVPVIAGVAEFTTDFARQTVREAARVG
                     VDGVMVMPALVYSARPHETAAHFRAVATGTDLPVMIYNNPPIYRNDVTPDVLIALQDC
                     ENIVCFKDSSGDTRRFIDLRNAVGDRFVLFAGLDDVVVESIAVGAEGWVSGMSNAFPK
                     EGETLFRLAKQKRFDEALALYRWFMPLLHLDARPDLVQCIKLCEALLGRGSAVTRPPR
                     LALQGETLAEVERIVEKALATRPALPDVGV"
     gene            complement(1219..2874)
                     /locus_tag="VHV99_10645"
     CDS             complement(1219..2874)
                     /locus_tag="VHV99_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HEX3635931.1"
                     /translation="MLSLSSCGDNNINSPAATPLSCAQLAGKTVPASAIGLPTTGATV
                     TAATTMPAAGSGAQATGEYCLVSGAINPVNSAAPQIKFQIAMPTVWNNKIMMFGGGGY
                     DGTIPAPTGNVPAGPTTQLTPLGRGYATFASDSGHQANALGSEDGSFGVNDEAVQNFS
                     GDALKKTRDAAVYLIDARYAVKAPKRAYFAGGSSGGREALAVVQRWPQDWDGSIVLYP
                     AWAAASLDLQFGRITRALAAPGAYLDQAKRKVLLNAVLATCDTLDGVADGLISNVAAC
                     NATFNPATATVNGTALRCAGGTDTGDTCLSDAQIAALNVYNTPITFGYSLASGETQYP
                     GFNVYGADLGVANASALQPTVTTLALGMSQPASPMPTTAPYLSVFWDQWIRYFVTRNP
                     NANSLSVDPQNPGSLQARISQLTGLQDVNKTDLSAFNAKGGKILMAHGMADALVSTRS
                     SEQYYQRLQATMGASAVANFVRFYEIPGYGHAVSTVYNAAWDSLTTLENWAEKGVTPP
                     AQTVADTAGVPGRTRPLCEFPSFPRYNGSGDMNVASSFTCATR"
     gene            complement(3160..>3498)
                     /locus_tag="VHV99_10650"
     CDS             complement(3160..>3498)
                     /locus_tag="VHV99_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX3635932.1"
                     /translation="AAPRDELKAHLQWIARDIAAPKYRGCPFINVATEFPAPDHPARA
                     VAVRHKAELRRRLGALAKQIGVARPDSLASQIALLIDGAYVCGQLARETAQALVPAAL
                     ALISVADKPG"
BASE COUNT          609 a         1055 c         1140 g          694 t
ORIGIN      
        1 gcgcattgaa acgctgctgc aaatcgggtc cgacgtgtaa tttttccttc aatcgactct
       61 ctgacatgcg tgcccgacct ggtgctttcc gagccgagct gagtttcgac tgtgcagatt
      121 tcatctcttt cgtgcgcgaa aagccccaag cccccacctg gataaaacct gatactcatt
      181 cacatattca acatgtgtcg gcatgaccga cctttacgaa ggagatgtgt accgtgagcc
      241 gcaacgctat ccaatggagc ggggtgtttc ccgccgtcag cacccagttc aagccggact
      301 tttcgctcga tgtcgatgcg acgcatcgtg tggtcagcaa cctggtcaag gacggcgtgt
      361 cgggcctcgt ggtctgcggc acggtcggtg agaatacgtc gctcagcacc gctgagaaga
      421 tggcggtgat cgaagccgcg cgcgacgcgg ccggcggcaa ggtaccggtg attgccggcg
      481 tcgccgagtt caccactgac ttcgcgcgcc agacggtgcg cgaagccgca cgcgtcggtg
      541 tcgacggcgt gatggtcatg ccggctctcg tctattcggc caggccgcac gagaccgccg
      601 cccatttccg cgcggtcgcc accggcaccg atctgccggt gatgatttac aacaacccgc
      661 cgatctacag gaacgacgtc acgccggacg tgctgatcgc cttgcaggac tgcgagaaca
      721 tcgtgtgctt caaggactcg tcgggcgata cgcggcgttt catcgatctg cgcaatgcgg
      781 tcggcgaccg cttcgtgctg ttcgcgggac tcgacgatgt ggtcgtggaa agtattgcgg
      841 tgggtgcgga gggttgggtg tcgggtatgt ccaatgcctt cccgaaggaa ggggaaacgt
      901 tgttccgcct cgcgaagcag aagcgctttg atgaagcgct ggcgctgtat cgctggttca
      961 tgccgctgtt gcatctcgat gcgcgtcccg acctcgtgca gtgcatcaag ctctgcgagg
     1021 cgctgctcgg gcgcggtagc gcggtcacgc ggccgcctcg cctcgcgctg caaggcgaga
     1081 cgctggccga ggtcgagcgc atcgtcgaaa aggcgctcgc aacgcggccg gcgctgccgg
     1141 atgtcggcgt ttaagcacaa cggcagagcg tttgcctcgc gtgatgggtc gcgaggcaaa
     1201 cctggattgc gcgctagatc accgcgttgc gcaggtgaaa ctcgacgcaa cgttcatatc
     1261 gccggaaccg ttataccgcg ggaagctcgg aaattcgcaa agcggccggg tacgtcccgg
     1321 cacacccgcc gtatccgcga cggtctgcgc gggcggtgtc acgccttttt cggcccagtt
     1381 ttccaaggtc gtgagcgagt cccatgctgc gttgtacacc gtgctgaccg catgcccata
     1441 gccgggaatc tcgtagaagc gcacgaagtt ggccaccgct gacgcgccca tggtcgcttg
     1501 caaacgctga tagtactgtt cactggagcg cgtgctaacg agcgcgtcgg ccatgccgtg
     1561 cgccatcagg atcttgccgc ctttcgcgtt gaacgccgac agatcggtct tgttgacgtc
     1621 ctgcaacccc gtcaattgac tgatacgcgc ttgcagactg cccggattct gcgggtcgac
     1681 gctcagcgag ttggcgttgg ggttacgcgt cacaaagtag cggatccact gatcccagaa
     1741 cacgctgaga tatggggcgg tcgtgggcat cggcgaggcc ggttgcgaca tgcccagcgc
     1801 gagcgtcgtg accgtgggtt gcaaggctga tgcattcgcg acgccaaggt ccgcgccgta
     1861 aacgttgaag ccgggatact gcgtttcacc gctggctagc gagtaaccga aggtgatcgg
     1921 cgtgttgtac acgttgagcg cggcgatctg cgcgtccgac agacaggtgt cgcccgtgtc
     1981 cgtgccgccg gcgcaacgta acgcagtgcc gttgaccgtc gccgtagcgg gattgaacgt
     2041 ggcgttgcag gctgcgacgt tgctgatcag gccgtcggcg acaccatcga gcgtgtcgca
     2101 ggtagccagc accgcgttga gcaggacttt gcgcttggcc tgatccagat aggcgccggg
     2161 tgccgcgagc gcgcgcgtga tgcggccgaa ctggaggtcg agactggcgg cggcccatgc
     2221 cggatagagg acgatcgagc cgtcccagtc ttgcggccag cgttgcacca cagcaagcgc
     2281 ttcgcggccg ccggacgagc cgcccgcgaa gtaggcgcgc ttcggcgcct tgaccgcata
     2341 gcgggcgtcg atcagataga cggcggcgtc gcgggtcttt ttcagcgcgt cgccggagaa
     2401 gttctgcacg gcttcgtcgt tgacgccgaa cgacccgtct tcgctgccca gcgcgttggc
     2461 ctgatgcccc gaatcgctcg cgaaggtcgc gtaaccgcgt ccgagcgggg tcagttgcgt
     2521 cgtagggccg gccggcacgt taccggtcgg cgcaggaatc gtaccgtcat aaccgccgcc
     2581 accgaacatc atgattttgt tgttccagac ggtgggcatg gcgatctgga atttgatttg
     2641 gggcgccgcc gaattcacag gattgatcgc gccgctcacc aggcaatatt ctcccgtcgc
     2701 ctgcgcgccg ctgcccgccg cgggcatggt ggttgctgcg gtcacggtcg cgccggtagt
     2761 gggcaagccg atggcagacg ccggcacggt cttgcctgca agctgggcgc aactgagggg
     2821 cgttgccgcg ggtgagttga tattgttgtc gccgcaacta cttagcgaca gcatgataag
     2881 tactggtgca acgaccaacg agaaaccgat gccgccgagt tttcggacgg tgtgcttcgt
     2941 ttgcatggcc tgtctccgtt atgggtaggc gcccgtctac ggttcgtctg cttcgcggat
     3001 gcaaatacgc agatgaggac gccttgcaaa gttggcgcgg cgcggccttt atgtcaatcg
     3061 tcttgtcact caacgagttt tatttcccac ctgaggcgtg cttgggcgag ccggttctga
     3121 ccaaaaccgg ctgatccaaa gtatggcgat tctcccggat cagccaggtt tgtctgcaac
     3181 cgaaatcagg gctagcgccg ccggcacgag cgcttgcgcc gtctccctgg caagctgtcc
     3241 gcaaacgtag gcgccgtcaa taagaagtgc gatctgactg gccaggctat ccggcctggc
     3301 aacgccgatt tgcttcgcta gcgcaccgag acggcggcgc aactcggcct tatgcctgac
     3361 ggccaccgcc cgcgccggat gatccggcgc cggaaactcg gttgccacgt tgataaatgg
     3421 gcagccgcgg tatttcggtg cagcgatgtc acgagcgatc cactgcaggt gagccttgag
     3481 ttcatctctg ggcgcggc
//
LOCUS       DATASH010000384         2780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1640511,
            whole genome shotgun sequence.
ACCESSION   DATASH010000384 DATASH010000000
VERSION     DATASH010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1640511"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..727
                     /locus_tag="VHV99_10655"
     CDS             77..727
                     /locus_tag="VHV99_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX3635933.1"
                     /translation="MNKLSKLAFIAATAVMAASAMAQSVPASRQATNDNWVNGTGEYV
                     WMNGTNELCWRDAFWTPATANAKCDGALVAQAPTPPAPVAPPAPAITSQKITYQADAL
                     FDFDKAILKPAGKEKLDDLASKIGALNLEVVVATGYTDRIGSDKYNDRLSLRRAQAVK
                     AYLVSKGIESNRIYTEGKGKRNPVTTGCNQKNHKQLIACLAPDRRVEVEVVGTSKQ"
     gene            945..1643
                     /gene="ubiG"
                     /locus_tag="VHV99_10660"
     CDS             945..1643
                     /gene="ubiG"
                     /locus_tag="VHV99_10660"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEX3635934.1"
                     /translation="MTNADPHELQKFSDLAHRWWDPNAEFKPLHELNPIRLNWINAHA
                     PLTGKTVLDIGCGGGILSESMAGLGAHVKGIDLSSQALGVADLHSLESGVTVNYEEIA
                     AEALAAREPGTYDAVTCMEMLEHVPEPGAIVEACKTLVKPGGWVFFSTLNRNVKSYLF
                     AVIGAEYIARMLPKGTHDYARFIRPSELASFVRAAGLRTADIKGIVYNPLSKHFTLSA
                     DTSVNYMLACRRDV"
     gene            1675..2388
                     /locus_tag="VHV99_10665"
     CDS             1675..2388
                     /locus_tag="VHV99_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX3635935.1"
                     /translation="MSDPTPLPQREDNDDALGLCQAVLFDLDGTLADTAPDLAAAVNK
                     MRHDRSLEMVPLEDLRPLASAGARGLIGGAFGIGPEDHEFASMREEFLANYEADLCIE
                     TTLFPGIAELLDDLDARGVRWGIVTNKAARLTEPLIAQLGLEERAGCVVSGDTTPHSK
                     PHPAPLLHAARELDVVPERTVYVGDDLRDVQAGFAAGMKTIAAAYGYCGNDIPPTRWH
                     AQHVVHSPAELQKLLRDIG"
     gene            2646..>2780
                     /gene="ssrA"
                     /locus_tag="VHV99_10670"
     tmRNA           2646..>2780
                     /gene="ssrA"
                     /locus_tag="VHV99_10670"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          568 a          848 c          842 g          522 t
ORIGIN      
        1 tcgagcaatg tatgacttgt cttcgtcaac aggctcgcag taaacctgcg gtaatctcaa
       61 tttcgagagg agaaatatga ataaactttc aaagctcgcg ttcattgcag ctaccgcagt
      121 tatggctgca tccgccatgg cacagtcggt gccggcgtcg cgacaagcta cgaacgataa
      181 ctgggtgaat ggtaccggcg aatacgtgtg gatgaacggc acgaacgaac tttgctggcg
      241 cgatgcattc tggacgccgg caacggccaa cgcaaagtgc gatggcgccc tggttgctca
      301 ggctccgacc ccgccggctc cggttgcacc gcctgcaccg gctatcacga gccaaaagat
      361 tacgtatcaa gctgacgctc tgtttgactt cgacaaggct atcctgaagc ccgctggcaa
      421 ggaaaagctg gacgatctgg catcgaagat cggcgcgctg aacctggaag tcgtggtcgc
      481 aacgggttac acggaccgca tcggttcgga caagtacaac gatcgtctgt cgctgcgccg
      541 tgctcaagct gtcaaggcat atctggtcag caagggcatc gagtccaacc gtatctatac
      601 ggaaggcaag ggcaagcgca acccggtcac gaccggttgc aaccagaaga accacaagca
      661 actcatcgcc tgcctcgcac cggatcgtcg cgtggaagtc gaagttgtcg gtacttcgaa
      721 gcagtaagcc acaagttacc gcagtatcaa agccccgctt cagcggggct ttttttatgc
      781 ccatgctcga gcctggttct cgcgccctcc tccgaccgcg ccccaatccg cagcaacaag
      841 acgtcgggcg tcgcgcagca ttttgccgcc cattcccttg ccctatatac tcagggttta
      901 tcctcgagcg cccgctcaac gctctcatcg aggcagcttc cgctatgacc aacgccgatc
      961 cccacgaact gcagaaattc agcgatctcg cgcaccggtg gtgggacccg aatgccgaat
     1021 tcaaaccgct gcatgaactc aatccgattc gcctgaactg gattaacgcg cacgcgcctc
     1081 ttaccggcaa gacggtgctg gatattggct gcggcggcgg gattctgtcg gagtcgatgg
     1141 caggattggg cgcgcacgtg aagggaatcg acctatcgag ccaggcactc ggcgtggcgg
     1201 atcttcacag ccttgaaagt ggtgtaaccg ttaattacga agaaatcgcg gctgaagcac
     1261 tcgctgcccg cgaacccggc acttacgacg ccgtcacctg catggaaatg ctcgagcacg
     1321 tgcccgaacc gggcgccatt gtcgaagcat gcaagacgct ggtgaaaccg ggcggctggg
     1381 tgttcttctc cacactgaat cgcaatgtga agtcgtatct gttcgcggtg atcggcgccg
     1441 aatatatcgc gcggatgctg cccaagggga cgcacgacta cgcacgcttc atccgtccgt
     1501 cggaattggc gagcttcgtg cgcgccgccg gcctgcgcac ggccgacatc aaaggtattg
     1561 tttacaaccc gctcagcaag catttcacgc tgtccgccga cacgagtgtg aactacatgc
     1621 tcgcctgccg ccgcgacgtc tgatccgccc gaccagccca gatcaattca atcaatgagc
     1681 gatcccacgc ccctgcccca acgcgaagac aatgacgatg cgctcggtct ttgccaggct
     1741 gtcctgttcg acctcgacgg cacgctggcc gacaccgcgc ccgacctggc ggctgccgtc
     1801 aataagatgc gccacgaccg gagcctcgag atggtgccgc tcgaagacct gcggccgctt
     1861 gcatcggcgg gcgcgcgcgg gctgatcggc ggcgccttcg gcatcggccc cgaggatcac
     1921 gagttcgcgt cgatgcgcga ggagtttctc gccaactacg aagcagattt gtgcatcgag
     1981 accacgctgt ttcccggcat cgccgaattg ctcgacgatc tcgacgcaag gggcgtgcgc
     2041 tggggcatcg tgacgaacaa ggcggcaagg ctcaccgaac cgctgatcgc gcaactcggc
     2101 ctcgaagagc gcgcgggctg cgtggtgagc ggcgacacga ccccgcattc gaagccacac
     2161 cccgcgccgt tgctgcacgc cgcgcgtgaa ctggatgtgg tgccggagcg caccgtctat
     2221 gttggcgacg acctgcgcga tgtgcaggcg ggtttcgcgg ccggcatgaa gaccattgcg
     2281 gccgcctacg gttattgcgg gaacgacatt ccgccaacgc gatggcacgc gcagcatgtc
     2341 gtgcactcgc ccgctgaatt gcagaagttg ctgcgcgata tcggctgagg gcagacgacc
     2401 acacggcggc ccgcgcgcag gcaacgtatt gcggcgcgtt gccgtgttag cgccatgggt
     2461 gttggcgtgt tggcgtgttg gcgtgttggc gtgttggcgt gtcggcgtgt cggcgtgtcg
     2521 gtgcggctgt gcggctgtgc ggctgtgcgg ctgtgcggca agataacaag ccaatcagtc
     2581 gggacgcggc cgtcaaacta ttgtaaaatc tacgcttggc ccataagtat tgggcaacaa
     2641 accgcggggg cgacctggtt tcgacagggg ttgcgaagcg gctagggcat gtcgaggacc
     2701 cgtcacctcg ttaatcaatg ggaaaaacgt aactgcaaac gacgatacgt tcgcactggc
     2761 agcctaaggg ccgccgtcct
//
LOCUS       DATASH010000385        17230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_56912,
            whole genome shotgun sequence.
ACCESSION   DATASH010000385 DATASH010000000
VERSION     DATASH010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 17230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17230
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56912"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /locus_tag="VHV99_10675"
     CDS             <1..991
                     /locus_tag="VHV99_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HEX3635936.1"
                     /translation="RLARRWSAAALFGAGCVATAALMAVSGLLADTHSLLVLRVMTGA
                     SSAAIFISGGVLAARLASARPHDAGLVLGLYYGGTGWGIVASSVLVPLTIFNVAHGWQ
                     FAWFALAAACAAFAAVAIGAARKIESVHAIGSSSTHATDATDAPRWPRFGPALAGYGL
                     FGVGYIGYMTFIVALLRNAGMSAVVVTGFYLLLGVATVVSARLWCDLLDRMRGGHALA
                     ILNALLAVATLLPALFTQPWVAFVSGVLFGATFLSAVASTTAFVRHNLPVSHWARGIS
                     AFTIVFAFGQIVGPMVIGWVSDGAGLARGLVYSALLLAAGAVLAACQKPLRGV"
     gene            1111..2256
                     /locus_tag="VHV99_10680"
     CDS             1111..2256
                     /locus_tag="VHV99_10680"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012218155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEX3635937.1"
                     /translation="MPAITCIEDLRVLAQQRVPRMFYDYADSGSWTESTYRANESDFH
                     RLKLRQRVAVNMAGRSTRTEMIGQEAAMPVALAPTGLTGMQHADGEILAARAAEKFGV
                     PFTLSTMSICSIEDVAAATSKPFWFQLYMMRDRDFIERLIERARAARCSALMLTLDLQ
                     ILGQRHKDIKNGLSAPPKLTPANLINLATKPRWCLGMLGTRRRTFGNIVGHAKGVGDL
                     SSLSSWTAEQFDPGLSWADVEWVKKLWGGKLILKGIMDVEDARLAADSGADALIVSNH
                     GGRQLDGAPSSISALPEIAREVGSRIEVWMDGGIRSGQDVLKAVALGAKGTLIGRSFL
                     YGLGAMGEAGVTQCLQIIQKELDITMAFCGHTDIRQVDERILLPDGR"
     gene            2422..2580
                     /locus_tag="VHV99_10685"
     CDS             2422..2580
                     /locus_tag="VHV99_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007584499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635938.1"
                     /translation="MTDSMTSFVVGIVVVLCIVVFTVARYRREHKDLPVARWLDTHPI
                     KDWLHHKH"
     gene            complement(2750..3223)
                     /locus_tag="VHV99_10690"
     CDS             complement(2750..3223)
                     /locus_tag="VHV99_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX3635939.1"
                     /translation="MTARNRPLDNQDRKILRELQKNARLSNAELAELVGMSTTACWNR
                     TRQLETEGYIRGYVALLDQQKLGFADVVLLEVTLDRHDDDALARFGAELATLTEVLEA
                     YLVSGEYDYLVKVAVDGTAGYERFLREKLYKIAGIRHSRSMFALRCMKSIPSVQI"
     gene            complement(3544..3915)
                     /locus_tag="VHV99_10695"
     CDS             complement(3544..3915)
                     /locus_tag="VHV99_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635940.1"
                     /translation="MANNEYMLSHQDMVKAVIIHQNIHEGFWTLNISFNVAATHGPQV
                     PDPCLALTIKGIGIMRASPADPLAINAALVNPPLSARVEGAVQITATVNGAHEGGVQF
                     CRDVYRHVALQSAAKAPNKAS"
     gene            4018..4266
                     /locus_tag="VHV99_10700"
     CDS             4018..4266
                     /locus_tag="VHV99_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635941.1"
                     /translation="MADASAPAREGSGRKKTPIARVALGRAVAGTGGAARNDLQVNCL
                     CRTGLMENEVHDDQYDQRDAKKPAEKIRHDVISFRIEV"
     gene            complement(4352..5407)
                     /locus_tag="VHV99_10705"
     CDS             complement(4352..5407)
                     /locus_tag="VHV99_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012429007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX3635942.1"
                     /translation="MSEPVSLSAAARARIPDIARVAGVSTATVDRVLNGREGVRAVTA
                     QRVLQAAAQLGYLLTPEAPATSASKPMRIAFLLPAGTNRYLHMLGDYIDFAHDQWNAL
                     DMKCRVHYVESFNPKELADRLLHYGQRADGVVFMALEHPVVRDAVNTLADQGVPAITL
                     ISDLSNARRLAYVGIDNRAAGRTAALLLGRFMGPRPSGKIAMLAGSLNYRGHEEREIG
                     FLHLIESTFPSVRVIGLREGHDDSERNYAQTRKLLEQHPDLAGIYNSGGGSDGVARAI
                     VEARSEQKILFVGHGLTPDTRALLIDGTMDALITQTPQAMVGNCLRIFRNVLEKRDAM
                     SDVKPVQFSIVLRENLP"
     gene            5606..6631
                     /locus_tag="VHV99_10710"
     CDS             5606..6631
                     /locus_tag="VHV99_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012429008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX3635943.1"
                     /translation="MTNLARRTLLRAAAAVPVAGALGFPAIVRAASGPEFVFKYGNNL
                     PLTHPLNVRSQEAANQIKEASKGRMEIRIFPNNQLGGDTDMLAQVRSGGIEMFTPSAL
                     VVSTLAPSVAINAVGFAFSDYNQVWSAMDGQLGAYVRAAMQKAGLESFERMWDNGFRQ
                     TTTSTGPITSAQNMRGLKIRVPVSPLSIDMFKGLGASPTSLQFSEVYSSLQTHIVDAQ
                     ENPLPIVQVAKLYEVQKYCSLTNHMWDGYWFVLNPRAWQRLPKDLQGIASDAFNQSAL
                     KQRDDVRKLNEAAVADLQSKGLAVNRPAPDTFRAALRQAGFYAEWKSKFGTQAWELLE
                     QSVGKLA"
     gene            6822..8651
                     /locus_tag="VHV99_10715"
     CDS             6822..8651
                     /locus_tag="VHV99_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX3635944.1"
                     /translation="MPRRWLRVFDRGLIALVEVCAAALLAVEIVVMLAGVICRYVLHQ
                     PLVWSDELAGILFLWLAMLGAVLALRRGEHMRMTAFVSRLSPQRRAFVDTLAIAASVA
                     LLALLIGPAYDYASGEAAIVTPALEISNAWRALALPIGAALMLIVGLIRLAQVSRMRD
                     VLIVLGIAALLGVVAVFGGPWFQTLGKANLVIFFVGVVAIGIFSGVPIGFSFALATFG
                     YLGLSTFTPLEVMVGRMDEGMSHLVLLAVPLFVFLGLLIEMTGMARAMIEFLASLVGH
                     VHGGLSFVLIGAMYLVSGISGSKVADMAAIAPVLFPEMKKRGASEGDLVALLATTGAQ
                     SETIPPSIVLITIGSVTGLSISALFTAGMLPGAVLALILCVVVWWRYRKEDLSGAQRF
                     SKRQIGRLFIVSLPALALPFVIRMAVVEGVATATEVSTIGIAYAMVIGLLVYRRFEWK
                     RLGRMLVDAATLSGAILFIIGCATAMAWALTQSGFSQDLAQLMGSMPGGAYGFLAVSI
                     VVFVMLGSVLEGIPAIVLFGPLLFPIARLAGVNEVHYAIVVILSMGVGLFSPPFGVGY
                     YSACAISKVNPDAGIRPIAGYMGALIVGLILVAAIPWISIGFL"
     gene            8755..9294
                     /locus_tag="VHV99_10720"
     CDS             8755..9294
                     /locus_tag="VHV99_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012429010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3635945.1"
                     /translation="MSRFFGEIRQAGYVVRDIEAAMDYWSRVLGVGPWFYNERVPIEN
                     YTYRGQSYDVHNSVALANSGPLQVELIQTRNDAPSMYRDFLAAGHTGLQHNAYWTTSF
                     DADLARLQAQGFKVAMSGEVGRNGRFVYFDTENHPGTVIELSEIAGPKGKLFDMIHAA
                     SLDWDGRDPVRRFPDLSAL"
     gene            9291..10589
                     /locus_tag="VHV99_10725"
     CDS             9291..10589
                     /locus_tag="VHV99_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-bisphosphate carboxylase large subunit
                     family protein"
                     /protein_id="HEX3635946.1"
                     /translation="MNAPLQRQAVNDDCLEADYLIETPLDPARVADVMAGEQSSGTFV
                     RVANESDALRARSRASVLRIEELEPAAQPSLPSAWLARQGTPGPWRRARVTLSFPLAN
                     IDANLPTLAATVAGNLYDLGEVTGMRLLSLRLPASYRARFELPRHGVAGTRALTQVAG
                     RPMIGTIIKPNVGLSAEETAALVRELCEAGVDFIKDDEVCANPMHAPLAERVRAVMRE
                     VRDYRERSGRPVMVAFNITDDLDAMRRHAELVEREGGSCVMASINWCGFSALQTLRRA
                     TPLVLHAHRNGYGMMSREPALGMSFHAYQTLWRLTGVDHMHVHGLAGKFAQSDTEVIE
                     SARDCATPLAPGCDDIVLPAFSSGQWAGTVQATFDAVRSTDLLFMSGGGILAHPDGPA
                     AGVTSVRQAWEAVQACTPLAVYAEHMPELRRALEFFGGRT"
     gene            10586..12001
                     /locus_tag="VHV99_10730"
     CDS             10586..12001
                     /locus_tag="VHV99_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HEX3635947.1"
                     /translation="MSDTREASDMRKASGDGPVYGFYGDDFTGATDTLAHLARAGLRT
                     MLFFAPPDAVRLSMLGRLDAVGIAGAARTMPPQVQHQELARAGAAFAALGVRVMHYKV
                     CSTFDSAPQTGSIGVAIRTLREYCANPLIAVVGGQPNLRRYCVFGELFAAAGADDRPQ
                     SIYRIDRHPTMSRHPVTPMNEADLRVHLQRQGLENVQSIDWRCYAGDDAALQTALDCR
                     LDSKPDAVLFDVLDDSHLRAIGRLIARHARTQAPLLAVGASSVAQAYASALNDAGERG
                     EVNVVPPSLARARGPVFVLAGSLSPLTEVQIAAAQSYLRVELDPLQMTGDAAADYLAT
                     RVAAIVGPLRDGRNVLAFTTRRAARATNATDAAEARGALPQLAHACASLLQQVLDKVS
                     LQRIGIAGGDTSSFAVRALDAWGLSYLAPLSAGVTVCRLHAERSDLDGMEIMLKGGQM
                     GDADLFEQLLNGYAGVEADKD"
     gene            complement(11998..12798)
                     /locus_tag="VHV99_10735"
     CDS             complement(11998..12798)
                     /locus_tag="VHV99_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3635948.1"
                     /translation="MEKLLDRQVALITGASSGIGSGVAQALANAGAAVVLNYHSHGEA
                     AEALADEIGKAGGTAIAVQADVADPDQVEKMFDAGRKQFESVDIVIANSGVQKDSAFA
                     DMTLDDWKSVLATNLTGQFLTTQAAVKEFRRRGPRPVSKALGKIICMSSVHEVIPWGG
                     HVNYAASKGGIQMFMKSLAQEVAPERIRVNSIAPGAIRTPINKDAWDTDTALGKLLKL
                     IPYGRVGETEDIGKAAVWLASDESDYVVGTTLFVDGGMTLYPGFVDNG"
     gene            complement(12952..13107)
                     /locus_tag="VHV99_10740"
     CDS             complement(12952..13107)
                     /locus_tag="VHV99_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018423731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HEX3635949.1"
                     /translation="MLGTILLIVLILLLIGAFPSWPHSRSWGYAPTGGIGIVVIVVIV
                     LLVAGVI"
     gene            complement(13835..14161)
                     /locus_tag="VHV99_10745"
     CDS             complement(13835..14161)
                     /locus_tag="VHV99_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635950.1"
                     /translation="MNMDEDIAHVARVMAPSLRAFRGEPVLPPAYWRKRLQRIQDEFP
                     LTQLQTFQIKHLLGELDRFEAERGPQHVPQAHYDYWRLALDGQEIRPKLLALRHSIEH
                     VESDPK"
     gene            complement(14564..16165)
                     /locus_tag="VHV99_10750"
     CDS             complement(14564..16165)
                     /locus_tag="VHV99_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012429017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3635951.1"
                     /translation="MTPDPDAPLPSGQLLPFRESLLAMLGVAFVSMLVALDQTIVGTA
                     LPRIVADLKGFDLYAWVATAYMLASVITIPIFGRLGDLFGRKPFLIAAILLFTGASVL
                     CGLATSMLLLVISRGLQGIGGGILVGTVFATVADLFPNPKLRLRWLVFVTSAFGVANM
                     IGPTLGGMLTQYGGWRLVFFVNVPVGLVSLLFVQRFLPPLHHLRRKGPVRLDWLGAFV
                     IAVTFGSFQLLIELFPKYGVNTITVVLAVVAASFAMTLWLWERRIAYPIIPVDMLLNR
                     KLSALFAMSVLGGFALFSLVFYVPLLFQGGYAMSAHDSGMLITPLLLGTTVGSVLNNR
                     IVTRIRRANGIMYAGFALSALACLSVVVLRGTEPHLVWMSCMGLSGLGLGLVATSLTV
                     CSQQIVPRDQLGAATALLQSLRTFGGMLGTVMTGALLGHLYTQGVHRSLASYQATQWF
                     KSFASPELLVDRAEQAALINRLVSAGHAGDAMMNSARDSLVESIHIGILVAGAAALVG
                     LCLAWFVPPVRVGYPEAEVVSEGNA"
     gene            complement(16197..16481)
                     /locus_tag="VHV99_10755"
     CDS             complement(16197..16481)
                     /locus_tag="VHV99_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007585480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 family protein"
                     /protein_id="HEX3635952.1"
                     /translation="MKFIVQWDGLPTAENSVIERFMKTGGQPPEGVKLLGRWHAIGGL
                     HGFAIVEADDTRGLSAMALDWGDLLTVSIFPALTDEDLGAALGAYQAARR"
     gene            complement(16985..17212)
                     /locus_tag="VHV99_10760"
     CDS             complement(16985..17212)
                     /locus_tag="VHV99_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013342347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635953.1"
                     /translation="MAKLVIKDLTDSVELDRQAMAAIVGGARIGAHVSVAVQVAPGSA
                     RVVEYPPGFPAAHQAIAGTPATAVPRILPRS"
BASE COUNT         3275 a         5170 c         5361 g         3424 t
ORIGIN      
        1 gcgactcgcg cgccgctggt cggctgctgc gttgttcggc gccggctgcg tcgccactgc
       61 ggctttgatg gccgtcagcg gtctgcttgc cgacacccat tcgctactcg tgttgcgcgt
      121 gatgaccggc gcgagcagtg cggcgatttt tatcagcggt ggcgtgcttg cggcgcggct
      181 tgcgtcggcg agaccccacg acgcggggct cgtgctcggc ctgtattacg gcgggaccgg
      241 ttggggcatc gtcgcttcgt cggtgctggt gccgctgacg attttcaacg tcgcgcacgg
      301 ctggcagttt gcgtggttcg ccctggcggc tgcatgcgcc gcgttcgccg cagtcgcgat
      361 cggcgcagcg aggaaaatcg aaagcgtgca cgcgattggg tcttcgtcca cgcacgcaac
      421 cgacgcaacc gacgcgccgc gttggccgcg tttcggtccc gcgctggcag gctatggatt
      481 gttcggtgtc ggctatatcg gctatatgac cttcatcgtc gcgctgctgc gcaacgcggg
      541 catgagcgcc gtggtggtaa cgggcttcta cctgttgctt ggcgtagcga ccgttgtatc
      601 ggcacgtctg tggtgcgatt tgctcgaccg catgcgcggc ggccacgcgc tcgcgatact
      661 caacgccctg ctcgcggtgg cgacgctgct gcccgctttg ttcacgcaac cgtgggtggc
      721 gtttgtttcc ggcgtgctgt tcggcgcgac gtttctctcg gccgtcgcgt cgaccacggc
      781 ttttgtacgc cacaatttgc cggtgagtca ctgggccagg ggcatcagcg cattcacgat
      841 cgtgtttgcg ttcggacaga tcgtcgggcc gatggtgatc ggctgggtct cggatggcgc
      901 cgggctcgca cgtggactgg tctattccgc cttgctgctt gcggccggtg cggtgttggc
      961 ggcttgccag aagcctttgc gcggagtctg agccggctcg gcgaggcacc cgcgaagccg
     1021 ctttgaattg tccccaggaa ttctccggaa tatcgggcga ctgtattaag aacgtacact
     1081 catggcttct tccctacgct gagagaagcc gtgcctgcca tcacctgcat cgaagattta
     1141 cgcgtacttg cccaacagcg tgtgccgcgc atgttctacg actacgccga cagcggctcg
     1201 tggaccgaaa gtacctaccg cgccaatgaa agcgacttcc atcgcctgaa gctacgccaa
     1261 cgcgtggcgg tcaacatggc cggccgcagc acgcgcacgg aaatgatcgg tcaggaggcc
     1321 gccatgccgg tggcgctcgc gccgacggga ttgaccggca tgcaacatgc cgacggcgaa
     1381 atcctcgctg cgcgcgcggc ggagaagttc ggcgtgccgt tcaccttgtc cacgatgagc
     1441 atctgctcga tcgaagacgt tgccgccgca accagtaagc cgttctggtt ccagctttat
     1501 atgatgcgcg accgcgattt cattgagcga ttgatcgagc gcgctcgcgc ggcacggtgt
     1561 agcgccttga tgctcacgct ggacctgcag atcctcggtc aacgccacaa ggacatcaag
     1621 aacggattgt cggcgccgcc caaattgacc cctgcgaacc tcatcaacct cgcgaccaag
     1681 ccacgctggt gcctcggcat gctcggcacc cggcggcgca ccttcggcaa catcgtcggt
     1741 catgcgaagg gcgtgggcga tctgtcgtcc ctgagttcgt ggaccgccga gcagttcgat
     1801 ccggggctga gctgggccga cgtcgaatgg gtcaagaagc tatggggcgg caagctcatt
     1861 ctgaaaggca tcatggatgt cgaagacgcg cgccttgccg cggacagcgg cgccgacgcg
     1921 ttgatcgtct ccaaccatgg cggccgtcaa ctcgacggtg cgccctcttc gatctcggcg
     1981 ctccccgaga tcgcacgcga ggtcggctcg cgcatcgaag tgtggatgga tggcggcatc
     2041 cgcagcggtc aggacgtgct gaaagctgtt gcgctcggcg ccaagggcac gctgatcggc
     2101 cgcagcttcc tctatggcct gggtgcaatg ggagaggcgg gagtgacaca gtgcctgcag
     2161 atcatccaga aggaactgga catcaccatg gccttttgcg gccacacgga tattcgtcag
     2221 gtggacgagc gcatcctgct gcctgacggg cgttgaaggc agatacccgg cgccgcattc
     2281 tgggtgccgg gacagaccag cgccgcggtc tccgggtgtg gtcgccgggt ttggggttgg
     2341 cgggatatcg atgagacggt cgttgctatt tctctggtcg cctaaaatat aggcatggtt
     2401 ctgacctcaa aggaggttgc catgaccgac tcgatgacca gttttgtagt aggaatcgtg
     2461 gtggtgctat gcatcgtggt atttacagtg gcgcgctaca ggcgggaaca caaagacctg
     2521 ccggttgctc gctggctgga cacgcatccg atcaaggact ggctgcacca taagcactga
     2581 atggcgcgac gtgatttctg gaagcgctga ggcgcgcgac tgctgtggag gcagcgatcg
     2641 cgtgtcggtc cacatcggac cgagcggcgc ttctacgcta cccggggggc gctcgtagat
     2701 ttcgaactag tcattcgctt gtccacacga tgcgaaaaac tccgcggcac taaatctgca
     2761 ccgacggaat actcttcata cagcgcagcg caaacatcga gcggctgtga cggatgccgg
     2821 cgatcttgta gagcttctcg cgcagaaacc gctcgtagcc cgccgtaccg tcgacggcca
     2881 ctttgaccag gtagtcgtat tcgcctgaca ccagatacgc ttccagcact tcagtcagcg
     2941 ttgccagttc ggcgccgaag cgggcgaggg catcgtcgtc gtgccggtcg agcgtaacct
     3001 cgagcagcac cacgtcggcg aagccgagct tctgctgatc gagcaacgcg acgtagccgc
     3061 gaatgtaccc ctcggtttcc aattggcgcg tgcgattcca gcacgcggtg gtcgacatgc
     3121 cgaccagttc ggcgagctcg gcattactca ggcgcgcgtt cttctgcaat tcgcgcagga
     3181 tcttccgatc ctggttgtcg agtgggcgat ttcgggcggt catggcattt tcggaagaat
     3241 gttcggataa ataccattct agcggaagat cattccgaaa ttggtgagca ggtggattgc
     3301 tttaagaagc atatccagcc tgcacccggg tatattttca gggtgcattc atgcccggga
     3361 caaccgtccg ggccgccggc ctcgggcctg cggtcttgcg cggccacaag ctgccatccg
     3421 gggtctcgct gccttcacag gaaggccgca cgcgagcgaa ttccggcgtt accgcgccgg
     3481 gagacgcgct cgccgggcat ggagcatttt ccctaaccga atgagcagat ggacagatag
     3541 cgtttagcta gctttgttcg gcgctttggc cgcgctctgt aacgcgacat gacgatagac
     3601 gtcgcgacag aattgcacgc cgccttcatg cgcgccattc acggtggccg tgatttgcac
     3661 agcaccttca acacgcgcgg acaagggcgg attgacgagg gcggcattga tggcgagcgg
     3721 atcggcaggc gaggctcgca tgatgccaat gcccttgatc gtcagggcga ggcacggatc
     3781 cggcacttgc ggaccatgcg tggcagcgac gttgaaactg atgttcagcg tccagaagcc
     3841 ctcatgaata ttctgatgaa taatcaccgc cttcaccatg tcctgatgcg acaacatgta
     3901 ttcattgttt gccatgggtc cctccttgct cgtacgcagc atgtgcgtgc gtaggcagat
     3961 atggcatttt gcgcgggcgg gtgagtctgg ttttacgcac agttttgcac agcgcaagtg
     4021 gccgacgcat ctgcgccggc gcgcgaagga agtgggcgca agaagacacc catcgcgcgg
     4081 gtggcgttgg gacgcgcggt cgccggaaca ggcggcgccg cgcgcaatga tcttcaggtg
     4141 aactgtctat gtcgcacagg attaatggaa aatgaggtac atgatgacca gtacgatcag
     4201 cgggacgcca agaagccagc cgagaagata aggcatgatg tgatctcctt tcgtatcgag
     4261 gtttgaatac gatggtgatt cagcatccgg cgtgcctgac ggtattcgct cacagccacg
     4321 cgcttgctga aaaggccgaa acccccgcta atcaaggcaa attctccctc agcacaatac
     4381 tgaactgcac cggcttcacg tcactcatcg catcgcgttt ttccagtacg ttgcggaaaa
     4441 tccgcaaaca gttccccacc atggcctgcg gcgtttgcgt gatcaacgcg tccatggttc
     4501 cgtcgatcag caacgccctc gtgtcaggcg tcaaaccgtg tccgacaaac aggatcttct
     4561 gctcggaacg cgcttcgaca atcgcgcgcg cgacgccgtc cgatccgccg ccactgttgt
     4621 aaatccccgc gagatcggga tgctgttcca gcagcttgcg cgtctgcgca taattgcgct
     4681 cgctgtcgtc gtgtccttca cgtagaccaa tcaccctgac cgaagggaac gtcgattcga
     4741 tcagatgtaa aaagccgatc tcacgttcct catgcccgcg atagttgagg ctgcctgcca
     4801 gcatcgcgat cttcccgctc ggccgcggcc ccatgaagcg ccccagcagc aacgccgccg
     4861 tgcgccccgc tgcgcgattg tcgatgccga cataggcgag gcgccgcgca ttcgacaaat
     4921 cggaaatcag cgtaatcgcc ggcacgccct gatcggccag cgtattcacc gcgtcgcgca
     4981 cgacgggatg ttcgagtgcc atgaacacca cgccatccgc gcgttggccg taatgcagca
     5041 gacggtcggc gagttctttc ggattgaaac tctcgacgta atggacgcgg catttcatgt
     5101 ccaacgcgtt ccactggtcg tgcgcgaagt cgatgtagtc gccgagcatg tgcaaatagc
     5161 gattggtgcc ggcaggcagg aggaacgcga tgcgcatcgg ctttgaagcg gacgtggcgg
     5221 gcgcctccgg cgtcagcaaa tagcccagtt gcgcggctgc ttgcagcaca cgttgcgcgg
     5281 tgacggcgcg taccccttcg cgaccattga gtacgcgatc caccgtggcg gtcgagacgc
     5341 cggcgacgcg cgcaatgtca ggaatgcgcg cccgtgcggc ggcgctgagg gaaaccggct
     5401 cggacatgac agtcccttcg cagagtgaat tccatcaaaa accatcaggt tcgcggtttg
     5461 acagcagtta ccgcggacgc ctagtcttgc agctatcgca cgacgactga tacgcagtga
     5521 ccggaaatta cagcagtgtc agcaggtgat gatggttttt gatggattcg acagtcacat
     5581 caattataag caaggagaca cccaaatgac caacctcgcg agacgcacat tgctgcgtgc
     5641 cgctgccgct gtgccggtag ccggcgcgct gggctttccg gcgatcgtgc gcgcggcgtc
     5701 cggcccggag ttcgtgttca agtacggcaa taacctgccg ctcacgcatc cgttgaacgt
     5761 gcgctcgcag gaagcggcga atcagatcaa agaggcgtcg aaggggcgca tggagatccg
     5821 gatattccct aacaaccagc ttggcggcga taccgacatg ctcgcgcaag tgcgcagcgg
     5881 cggcatcgag atgttcacgc cgtcagcgct ggtggtgtcg acgctcgcgc cgtcggtggc
     5941 gatcaacgcg gtcggctttg ccttcagcga ttacaaccag gtgtggagcg cgatggacgg
     6001 ccagctcggc gcctatgtgc gcgcggcgat gcagaaggcc gggctcgagt cgtttgaaag
     6061 gatgtgggac aacggttttc gtcagaccac taccagcact ggcccgatca cgagcgcgca
     6121 gaacatgcgc ggtctgaaga tccgcgtgcc ggtgagtccg ctcagcatcg acatgttcaa
     6181 aggcctcggt gcttcaccga cgagtctgca attcagcgaa gtctattcat cgctgcaaac
     6241 gcatatcgtc gatgctcagg agaatccgct gccgatcgtg caggtcgcga aactctacga
     6301 agtacagaag tattgttcgt tgaccaacca catgtgggac ggctactggt tcgtcctgaa
     6361 cccgcgcgcg tggcaacgct tgccgaagga tctgcagggc attgcaagcg atgcgttcaa
     6421 tcaaagcgcg ttgaaacaac gcgatgacgt tcgcaagctc aacgaggctg ccgtcgcgga
     6481 cctgcagagc aagggcctcg cggtcaatcg tccggcgccg gacacgtttc gcgcggcact
     6541 gcgtcaagca ggcttctacg ccgaatggaa gagcaagttc ggcacgcagg cgtgggagct
     6601 gctcgaacaa tcggtcggca agctggctta agcgtgagcc tggaacccgc tgtgaaccgt
     6661 gtgctgtgag cctcgcgctg tgaccatgaa tacggagccc gaccgtgcgg cacatcggtg
     6721 agcatgagac gaagcgcggc gagtggccgc gaggaggcac cagcatgtcg aatcacgtat
     6781 tgaatcctgc cgaggcggcc gtcgcgttag acgccgcgag catgccgcgc cgctggctga
     6841 gagtcttcga tcgtggcctg atcgcgctcg tcgaagtctg tgcggcggcc ttgctggcgg
     6901 tggagatcgt cgtgatgctg gcgggcgtga tctgccgcta cgtgctgcac cagccgctgg
     6961 tgtggtcgga cgaactcgcg gggattctgt tcctgtggct cgccatgctc ggcgccgtgc
     7021 tcgccttgcg gcgcggcgag catatgcgga tgacggcttt tgtcagccgt ctttcgccgc
     7081 agcgccgcgc gttcgtcgac acgctggcga ttgctgcgtc ggttgcattg ctcgcgctgt
     7141 tgatcggacc cgcatacgac tacgcgtccg gcgaggcggc gatcgtcacg cccgcgctgg
     7201 agatcagcaa cgcatggcgc gcgctcgcgt tgccgatcgg tgccgcactc atgctgatcg
     7261 tcggcctgat tcgtctcgcg caggtgagcc ggatgcgcga tgtgctgatc gtgctcggga
     7321 ttgccgcact gttgggcgtg gtagcggtgt tcggcggtcc gtggttccag acgctcggca
     7381 aagccaatct ggtgatcttc ttcgttggcg tggtcgcgat cggtattttt tcgggcgtac
     7441 ccatcggctt ttctttcgcg ctcgccacgt tcggctatct cgggctctcg actttcacgc
     7501 cgctcgaagt gatggtcggc cgcatggacg aggggatgtc gcatctggtg ttgctggcgg
     7561 tgccgctgtt cgtctttctc ggcttgctga tcgaaatgac cggcatggcg cgcgcgatga
     7621 tcgagtttct cgcgagtctg gtcggtcacg tgcacggcgg tttgtcgttc gtgctgatcg
     7681 gcgcgatgta tctggtgtcg ggcatctccg gttcgaaagt cgcggacatg gcggcgatcg
     7741 cgccggtgct gttccccgaa atgaaaaaac gcggcgcctc ggaaggcgat ctggtcgccc
     7801 tgcttgccac caccggcgcg caaagcgaaa cgatcccgcc gagcatcgtg ttgattacga
     7861 tcgggtcggt gaccggtctg tcgatctccg cgctcttcac cgccgggatg ctgccgggtg
     7921 ccgtgctcgc gttgattctg tgcgtggtgg tgtggtggcg ttatcgcaag gaggacctga
     7981 gcggcgcgca acggttcagc aagcggcaga tcgggcgtct gttcatcgtg tcgctgcccg
     8041 cgctggcgtt gccgttcgtg atcagaatgg cggtggtcga aggcgtggcc acggcgaccg
     8101 aagtgtcgac catcggcatt gcgtatgcga tggtgatcgg gctcctggtg taccggcgct
     8161 tcgaatggaa gcgccttggc cgcatgctgg tggatgccgc gaccttgtcg ggcgcgatcc
     8221 tgttcatcat cggatgcgcg acggccatgg cgtgggcgct gacgcaatcc ggcttctcgc
     8281 aggacctcgc gcaattgatg ggctcgatgc caggcggcgc gtatggcttt ctggcggtgt
     8341 cgatcgtcgt gttcgtgatg ctcggcagtg tgctcgaagg catccccgcc atcgtgctgt
     8401 tcggaccgct gctgtttccg atcgcgcggc tcgccggcgt caatgaagtg cactacgcga
     8461 tcgtcgtgat cctctcgatg ggcgtgggtc tgttctcgcc gccgttcggc gttggctact
     8521 actcggcatg cgcgatcagc aaggtgaatc ccgatgcggg cattcgtccc attgccgggt
     8581 atatgggcgc tttgattgtc ggcctgattc tggtcgcggc gattccctgg atttctatcg
     8641 gttttctcta aggtctcttt taagtctctt aacggggtcg acgttcgggc gctgcgttgc
     8701 cgctgcgccg gggcgtcgct cgtcttttaa aactggcagc atcgggagag tttcatgagt
     8761 cgtttctttg gcgagatccg ccaggccggg tacgtagtgc gcgatatcga ggccgccatg
     8821 gactattgga gccgcgtgct cggcgtgggt ccgtggttct acaacgaacg cgtgccgatc
     8881 gagaattaca cgtatcgcgg gcaatcgtac gacgtgcata actcggtggc gctggcgaac
     8941 tcgggaccct tgcaggttga actgatccag acgcgcaacg atgcaccgtc catgtatcgc
     9001 gactttctcg ccgcgggtca taccggcttg cagcacaacg cgtactggac cacttcgttc
     9061 gacgccgacc ttgcccgttt gcaggcgcaa ggtttcaaag tcgcgatgag cggcgaggtg
     9121 gggcgcaacg ggcgcttcgt atacttcgac acggaaaatc atccgggcac ggtgatcgag
     9181 ttgtcggaaa tcgccgggcc gaaaggcaaa ctgttcgaca tgatccatgc ggcctcgctc
     9241 gattgggacg gtcgtgatcc ggtgcgccgg tttcctgatc tgagcgcgct atgaacgcgc
     9301 cgctgcaacg gcaggccgtc aacgacgatt gcctcgaagc cgactacctg atcgagacgc
     9361 cgctcgatcc ggcccgcgtc gccgacgtga tggccggcga gcagtcgagc ggcacgtttg
     9421 tgcgggtcgc caacgagtcc gacgcgctgc gcgcgcgcag ccgcgcgagc gtgctgcgca
     9481 tcgaagaact cgagccggcc gcacagccca gcttgccgag cgcatggctc gcacgtcagg
     9541 gtacgccagg accgtggcgg cgcgcgcgcg tcacgctgtc gttcccgctt gccaatatcg
     9601 atgcgaatct gccgacgctt gccgcgaccg tcgccggcaa tctgtatgac ctcggcgaag
     9661 tgacaggcat gcggctgttg tcgttgcgct tgccggcgtc ttaccgggct cgctttgagt
     9721 taccgcgcca tggtgtggcc ggtacgcggg cgctgacgca agtcgcgggc agaccgatga
     9781 tcggcaccat catcaagccg aacgtcggtc tgagcgcgga agaaactgcc gcactcgtgc
     9841 gcgagttatg cgaggcgggc gtcgatttca tcaaggacga tgaagtgtgc gcgaatccca
     9901 tgcacgcccc gctcgcggaa cgcgtgcgtg ccgtgatgag ggaagtgcgc gactatcgcg
     9961 agcgtagcgg ccgtccggtc atggtcgcct tcaatatcac cgacgacctc gacgccatgc
    10021 gccgtcacgc ggaactcgtc gagcgggaag gcggcagttg cgtgatggcc agcatcaact
    10081 ggtgcggctt ttccgcgctc cagacactgc gtcgcgcgac accgctcgta ctgcacgcgc
    10141 atcgcaacgg ctacggcatg atgtcgcgcg agcccgcgct cgggatgtcg tttcacgcgt
    10201 atcagacgct gtggcgtttg acgggtgtcg atcatatgca cgtgcacggc ctcgccggaa
    10261 aattcgccca gagcgacact gaggtgatcg agtccgcgcg cgattgcgct acgccgctcg
    10321 cgccaggttg cgacgatatc gtgctgcccg cgttttcgtc aggacaatgg gccggcaccg
    10381 tgcaagcgac attcgacgcc gtgcgctcca ccgatttgct gttcatgtcg ggcggcggca
    10441 ttctcgcgca tccggacggt cccgcggcag gcgtcacgag cgttcgacag gcttgggaag
    10501 ccgtgcaggc gtgcacgccg ctggcggttt acgctgaaca catgccggag ctgcggcgtg
    10561 cgttggaatt tttcggtggc cgcacatgag cgacacacgg gaggcgagcg acatgcgtaa
    10621 ggccagcggc gatggccctg tctacggctt ctacggtgac gactttaccg gcgcgaccga
    10681 cacgctcgct catctcgcgc gcgccggttt gcgcaccatg ctgttcttcg cgccgccgga
    10741 tgccgtgcgg ctttcgatgc tgggccgcct cgacgcggta ggtatcgcgg gcgccgcgcg
    10801 gaccatgccg ccgcaggtgc agcatcagga actggcgcgc gcgggggccg cgttcgccgc
    10861 gcttggtgta cgcgtgatgc actacaaggt gtgctcgact ttcgatagcg cgccgcagac
    10921 cggcagcatc ggtgtggcga ttcgcacgtt gcgcgagtac tgcgcgaatc cgttgatcgc
    10981 ggtggtgggc ggtcaaccga atctgcgtcg ctattgcgtg ttcggcgaac tgttcgcggc
    11041 agccggcgcg gacgatcgtc cacagtcgat ttacagaatc gaccgtcatc cgaccatgag
    11101 ccgtcatccc gttacgccga tgaatgaagc ggacttgcga gttcatttgc agcgccaagg
    11161 gctggagaat gtgcaatcga tcgactggcg ttgctatgca ggcgacgacg cggcgctgca
    11221 aacggcgttg gattgccggc tcgactcgaa gccggacgcc gtgctgttcg acgtgctcga
    11281 cgactcgcat ttgcgggcga ttggacggtt gattgctcgt catgccagga cgcaggcgcc
    11341 attgctcgcg gttggggcga gcagcgtggc gcaggcttat gcgagtgcgc tcaacgacgc
    11401 gggtgaacgt ggggaagtaa acgttgtgcc cccgtcgctg gcgcgcgcgc gcggaccggt
    11461 gttcgtgctg gccggcagtc tctcgccgtt gaccgaagtg cagatcgccg cggcgcagtc
    11521 ttatctacgt gtggaacttg accccttgca aatgactggc gatgccgcag cggattatct
    11581 ggctacgcgt gttgccgcca tcgttgggcc gttgcgtgac ggccgcaatg tgctcgcttt
    11641 cacgacgcgt cgtgcggcgc gcgcaacgaa tgcgactgac gcggctgaag ctcgcggggc
    11701 gcttcctcaa cttgcccacg catgcgcctc gttgctgcaa caggtactcg acaaggtgag
    11761 cttgcaacgc atcgggatcg cgggcggcga tacatcgagc tttgccgtcc gcgcactcga
    11821 cgcctggggg ctgtcgtatc tcgcgccctt atcggcgggc gtcacggtgt gccgcctgca
    11881 cgccgagcgg agcgacctcg acggcatgga aatcatgctc aagggcggcc aaatgggcga
    11941 tgccgatctg ttcgagcaac tgttgaacgg ctacgccggc gttgaggcag acaaggacta
    12001 accgttgtcg acaaagccgg ggtacaacgt catgccgccg tccacgaata gtgtcgtgcc
    12061 caccacgtaa tcgctttcgt ccgacgcgag ccagacagcg gctttgccga tatcctccgt
    12121 ttcgcccacg cgtccgtagg gaatcagctt caacaatttg ccgagcgcgg tgtccgtatc
    12181 ccacgcatcc ttgttaatcg gtgtacgaat cgcgccaggc gcaatggaat tcacgcgaat
    12241 gcgctcgggc gcgacctctt gtgccagtga tttcataaac atctgaatgc cgcctttgga
    12301 cgccgcgtaa ttgacgtggc cgccccaagg gatcacttcg tgcactgagc tcatacagat
    12361 gatttttcca agtgctttgg aaaccggccg cggcccgcgt cgccgaaact ctttcacggc
    12421 tgcctgggtg gtcaggaact ggccggtgag attggtcgcc agaacgcttt tccaatcatc
    12481 gagcgtcata tcggcgaacg cactgtcttt ttgtacaccg gaattggcga tgacgatatc
    12541 gacgctctcg aactgtttgc ggcccgcgtc gaacatcttt tcgacctgat caggatcggc
    12601 cacgtcggct tgaacggcga tcgccgtacc accagccttg ccaatctcat cggcaagcgc
    12661 ttccgccgct tcgccatgag aatgataatt cagcacgacg gccgcgcccg catttgcgag
    12721 cgcttgcgca acgcccgacc cgatgcccga actggcgcct gtgatcaaag cgacctgacg
    12781 gtcgagtaac ttttccattg cactctccgg cgtaacaagg tgtgtcgatc gactaccgat
    12841 ggatacccaa cgaatactga cataacgagg ccgccggcta ttgatgcagc gaactgcatc
    12901 gatagccggc ggccggctcg gttcataccc gaagcgagtg cttcaattcg gctatatcac
    12961 gcccgcgacg agtagcacga tcaccacgat caccacgatg ccaataccgc cggtcggcgc
    13021 ataaccccac gaacggctat gcggccacga cggaaacgca ccgatgagaa gaaggatcag
    13081 cacaatcaga aggatagttc ccaacatata aataacctcc gcttgggttg ataagacctg
    13141 cctgtcacga cagccatgca gtgcgcgccg aggtgagctt gcgaaaaggc agtatcgatg
    13201 tcttgggagt gagcaatcca tgtgcccacg ggccggcggc tgttacgggc ggtattacgt
    13261 aacgagcgcg ttactgaaca cgattccgca catttttacc ggcttcctga agcgtggcgt
    13321 ccgtagaaga acgtatatcc gacaagggtt tgcacgatgc tctgaggaca attgcgaaag
    13381 aaacctgcag attggcatgc ttgctgcttt agagattgcg cggtgcacaa cacagacaag
    13441 acaggcagta agtgctggtg gagttcgagt caacgaccgg cctgatcggg ggttatcagg
    13501 tcggtctcct caaccaggtc tacaagtttg atcccgaaac acttgcggtt atcggcgttg
    13561 ggccacctgc ggcgattgga cagtcggcta agctgccagg ttttgcacca cactatggat
    13621 cgtatcaact gccgattctc gcgtcgcctg gcgggaggcc aaccaggtac aagctggtgt
    13681 ggggagctct gcctcctggc aatcacgacg agccgagaag ttgcgatact ccatgcgcct
    13741 taccagattg cgagcttgtt tcagagcttt atcttgtggt tttgactacc gcgatgttag
    13801 tgatgcgcta gctggcttac cggtgacata tcgatcattt cggatcggat tcaacatgtt
    13861 ctattgaatg acgaagcgct aaaagctttg ggcggatttc ctgcccgtcc aaagcgaggc
    13921 gccaataatc ataatgagcc tgcggaacat gttgcggccc ccgctccgcc tcaaaccgat
    13981 ccaactcccc cagcaaatgc ttgatctgaa acgtctgcaa ctgagtcagc ggaaactcat
    14041 cctgtatgcg ttgtagccgc tttcgccaat aagcgggtgg cagcacaggt tcaccacgaa
    14101 aggcgcgcag cgacggcgcc attacacgcg cgacgtgcgc gatgtcttca tccatattca
    14161 tgcgagagcc ccgaaaaacg tcgatgtcta gttttgtttt ttctgtgtgc gctttgggtg
    14221 acaacgagtt tagtggctgc gcaaacgtcg acgctcatag taagaaatca gaatcgccag
    14281 gtcaaggttt tcgaccgaat ttagcatgga caacgcttgg aatttgcctt gatttcaggg
    14341 ctatttcgat cacgccaagg tctctatgag tagttatccg ggccttcaac gggcgtactt
    14401 cttctcgcga gattgctccg gcctgacacg cgtcaagcgg cgcaagtgag tgcctccaaa
    14461 cctcgagacg caggcagaaa aacagaggat gagtgaaaac tggggaaccc ggcaaagctg
    14521 gtgccgcttg aagcgcatta ccccgcggcg gcgccgcgtc ctcttacgcg tttccttcgc
    14581 tcaccacttc cgcttccgga tagccaaccc gcaccggcgg cacaaaccac gcgaggcaca
    14641 acccaaccaa cgcagccgcg cccgccacca gaatgccgat atgaatcgac tccacgagcg
    14701 aatcgcgcgc ggaattcatc atcgcatcgc ccgcatgtcc agcgcttacc aggcgattga
    14761 tcaacgccgc ctgctccgcg cggtccacca gcaactcggg actcgcaaac gatttgaacc
    14821 actgcgtcgc ctgatacgaa gcgagagagc gatgcacgcc ttgggtgtag agatgcccaa
    14881 gcaacgcacc cgtcatcacc gtccctagca tgccaccaaa agtccgcagc gattgcagca
    14941 acgcagttgc cgcgccgagc tggtcgcgcg ggacgatctg ttgcgaacag accgtcaagc
    15001 tggtcgccac cagaccaagt ccgagcccgc tgagacccat gcacgacatc cacaccaggt
    15061 gcggctcggt ccctctgagt acgacgacgc tcaaacacgc cagcgcggac agcgcaaaac
    15121 cggcgtacat gatgccgttc gcgcgccgaa tccgtgtgac gatacgattg ttcaacacgc
    15181 tgccaaccgt cgtgcccagc agcagcggcg taatcagcat ccccgagtcg tgggcggaca
    15241 tcgcgtagcc gccttgaaac aacagcggca cgtaaaacac cagcgaaaac agcgcaaaac
    15301 cgccgagcac tgacatcgca aagagggcgg agagcttgcg gttcaacagc atatccactg
    15361 gaatgatcgg ataggcgatc ctccgctccc acaaccatag cgtcattgca aaactcgccg
    15421 caacgacggc cagcacgacc gttatcgtat tgacgccgta cttcggaaac aattcgatca
    15481 gtaattggaa tgagccgaac gtgaccgcga tcacgaacgc gccgagccaa tcgagccgta
    15541 cggggccttt gcgccgcaga tgatgcagcg gcggcaagaa acgctgcacg aacagcagcg
    15601 acacgaggcc aaccggcaca ttgacaaaaa acaccagccg ccagccgccg tattgcgtga
    15661 gcatgccccc gagcgtcggt ccgatcatat tggcgacgcc gaatgctgac gtaacgaaca
    15721 cgagccagcg caaccgcagc ttcggattgg ggaacagatc ggcgacggtg gcgaacaccg
    15781 taccgaccag aatgccgccg ccaatcccct gcaggccgcg cgaaatcacc agtaacagca
    15841 tactggtggc gaggccgcac aataccgacg cgccggtaaa caacagaatc gcggcgatca
    15901 gaaacggctt gcgcccgaac agatcaccca gtcgcccgaa aatcggaatc gtgatcaccg
    15961 aagctagcat gtacgccgtc gcgacccatg cgtacagatc gaagcccttc agatcggcga
    16021 cgatgcgcgg tagcgcggta ccgacgatag tttgatcgag cgctaccagc atcgagacga
    16081 aggcgacacc gagcatcgcc agcaacgact cgcgaaacgg cagcaattgt ccgctgggca
    16141 gaggcgcgtc cggatcggga gtcatgacag tccgatttgc gcgctcatgc gtccacttac
    16201 ctgcgggcgg cctgatacgc gcccaatgca gcgccgagat cctcgtcagt caatgccggg
    16261 aagatgctca ctgtcagtaa atcgccccag tcgagtgcca tcgccgacag ccctcgcgta
    16321 tcgtccgcct cgacgatcgc aaagccatgc agtccgccga tcgcgtgcca gcgcccgagc
    16381 agtttgacac cttcaggcgg ttggccgccg gttttcataa aacgctcgat cacggagttt
    16441 tccgcggtcg gcaggccatc ccattgcacg atgaatttca ttgcatgtct ccagtttgga
    16501 ttggcgatgc cggctcgtgg cgcgctgcaa tcgtcctcag tgctgtgaga cgcatttgca
    16561 tgcctcgcgc cttgaacact ttcgcgccag gatgctgtcc ggttagcacg tgcgttatcg
    16621 gcggacggca taaaaccacg tggcgtcata aacagcatag gtgagcgaac ggccatcaac
    16681 gtcgccccat gcggacattc accaggccaa ttgaacaatg cgcgttgccg ggaaagcgtg
    16741 acgggcccgt ttttttccaa ttgtggccat ctcgcgcgtc ctataagaag gagcttcttt
    16801 tatacggctg tcgagaatgt ggatgtcgat ctgtcttgaa gcgcgccatg caatgcgccg
    16861 gcacgactgg cacacatgcg gatggcaaat ggaacaagct aaaacgcgtc agatacatca
    16921 aacagtaacg gcacgcgcgt caagcgcgtg ccgttactgt tgctgcgagt atccgaaccc
    16981 ggttttagct gcgcggcaaa atgcgcggca ctgcggtagc gggtgtaccg gcaatagctt
    17041 gatgcgctgc gggaaaaccc ggtgggtatt cgacaacgcg tgcggaaccc ggcgccacct
    17101 gcacagcgac actcacatgc gcaccgatcc gcgcgccgcc aacaatggcc gccatcgcct
    17161 ggcggtcgag ttcgacgctg tcagtaaggt ccttgatgac aagctttgcc atggcggctc
    17221 caggaaagag
//
LOCUS       DATASH010000386         9268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2235022,
            whole genome shotgun sequence.
ACCESSION   DATASH010000386 DATASH010000000
VERSION     DATASH010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 9268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9268
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2235022"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..69)
                     /locus_tag="VHV99_10765"
     CDS             complement(<1..69)
                     /locus_tag="VHV99_10765"
                     /inference="COORDINATES: protein motif:HMM:NF012951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HEX3635954.1"
                     /translation="MTTSVRTRFAPSPTGFIHLGNIR"
     gene            618..1568
                     /locus_tag="VHV99_10770"
     CDS             618..1568
                     /locus_tag="VHV99_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX3635955.1"
                     /translation="MKPSPPRLARRNFSLAAASAVLAACTTTGGGKTDSTPVAATPTT
                     NTPPLDKPQRPIRVGLALGGGAARGFAHIGVIKALEARNIQVDLVAGTSAGSVVGALY
                     ASGMNGFALNKLALTMDEASISDWAMPFRTRGFLQGIALQNYLNTTLNNRPIEKMVKP
                     LGVVATDLKTGQPILFQRGNTGIAVRASCSVPSIFEPVKIGGHEYVDGGLVSPVPASF
                     ARKMGADFVIAVDISQRPESGLTASSFDVLMQTFTIMGQTIKAYELDKYADIVIRPNL
                     AAMSGSDFSQRNAAILAGEEAVAKMMSELQRKLATSRAAV"
     gene            complement(1772..2437)
                     /locus_tag="VHV99_10775"
     CDS             complement(1772..2437)
                     /locus_tag="VHV99_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEX3635956.1"
                     /translation="MQHRNLTQACTRVVAGMFIGVLMAAAPGAFADEVSSFNQNASYS
                     TPNGSDSSSPLNSQSAAPSSDRGAKSFLSGMAGKAGDVVVGALNMIGVRYRWGGNTPD
                     SGLDCSGFVRYVFQDTLGMALPRRAEEMSRVGEKVRVSDLKPGDLVFFNTMRRTFSHV
                     GIYIGDNKFVHSPSTGSTIRVDDLDSGYWEKRFTGARRIETSYQDGEDLRKRVSATIG
                     GNQ"
     gene            complement(2575..4173)
                     /locus_tag="VHV99_10780"
     CDS             complement(2575..4173)
                     /locus_tag="VHV99_10780"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEX3635957.1"
                     /translation="MTPPLLELDHLHVTFGDTVAVNDVTLAIQRGERVALVGESGSGK
                     SVTALSILRLLSDAQVSGAIRFDGQDLLAKSEREMRGMRGSDIAMIFQEPMTALNPLY
                     TVGDQIAETIVIHDGVTANEARKRAVALLGRTGIAEPGKRVNSYPHQLSGGQRQRAMI
                     AMALACRPRLLLADEPTTALDVTIRAQIVDLLLELQRDEAEKRGMAVLLITHDLNLVR
                     HFAQRIAVMEMGILVESGPVEQVFESPQHPYTQRLLASRPQRTVVPVLPISPVLLEAR
                     DVSVDFKTKLPGVSGWFRSGRFRAVADANVSVRQGETLGIVGESGSGKSTLAMALLGL
                     QRTVHGEIEFQGRALGSYRGAEQTALRSNMQVVFQDPFSSLSPRQTIERIVGEGLALH
                     RPQMSEAARRDKVVSVLREVGLDRTVLYRYPHEFSGGQRQRIAIARALVLEPRILILD
                     EPTSALDVSIQQQVLKLLAGLQQKYNLGFVFISHDLAVIGAMAHRVAVMQNGSIVESG
                     NVEQIFATPVHAYTRKLLKAALDR"
     gene            complement(4206..5312)
                     /locus_tag="VHV99_10785"
     CDS             complement(4206..5312)
                     /locus_tag="VHV99_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3635958.1"
                     /translation="MNRARLSADASARTEPARAFVSPTPARRVWQRFRQQRLGYWSLI
                     IFVVAFAASLVGPLWSNDKPLVVRYEGQLYFPMFKTYAETTFGGDFPTPADYLDPYIK
                     HRFDAPGNFAVYPPNRYYYDTLNYFSKAPNPAPPSRDNWFGTDDRGRDLFARLLYGFR
                     VSVEFALVLTLIGTVLGIAAGAVQGYFGGRTDIIGQRLIEIWSAMPELYLLIIFSSIF
                     EPGFILLIVLLSLFGWIGLSDYVRAEFLRNRHQDYVRAARAMGLSNWQIMWRHVLPNS
                     LTPVITFLPFRMSGAILALTSLDFLGLGVPSPTPSLGELLAQGKANLDAWWISLSTFG
                     VLVAMLLLLTFMGDALRNALDTRISDAMRAGGNQ"
     gene            complement(5312..6349)
                     /locus_tag="VHV99_10790"
     CDS             complement(5312..6349)
                     /locus_tag="VHV99_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX3635959.1"
                     /translation="MWSYILKRLLLMIPTLLGVLTLTFAVIQFVPGGPVEQMQHELRK
                     GAENGAPFGLRAHTGVDAQQIAQLKALYGFDKPPLERYVLMLKRFSTFDLGQSYFRHQ
                     SVWSLIVSKLPVSISIGLWTFFLTYLISVPLGIAKAVRNGSRFDIATSLVVLIGYAIP
                     GFVLGVLLLVLFGGGSFLQLFPLRNLTSDNWAQLSLGGKIVDYLWHITLPITASVVGS
                     FAVVTMLTKNAFLDEIRKQYVLTARAKGLSEKRVLWKHVFRNALLPLIVGFPAAFIGA
                     FFTGSLLIETLFSLDGLGLLSYESVVRRDYPVVLGTLYLFTLIGLATKLISDLCYVWV
                     DPRIQFEQLER"
     gene            complement(6393..8360)
                     /locus_tag="VHV99_10795"
     CDS             complement(6393..8360)
                     /locus_tag="VHV99_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX3635960.1"
                     /translation="MTTGSRWVQTPRAPGCSVLHRRSRVGVPVVLRLVPRSWRSACLA
                     ALCGVSALAGLLAAPEAHAVYAIAQYGEPKYPADFKHFDYVNPDAPKGGTLVMANPSR
                     LTSFDKFNPFTLRGNTAPGVDLLFESLTTGSSDEVASAYGLLADDISIAPDGLSTTFH
                     INPRARFSNGDPVTADDVKFSLDTLKSPQAAPQFASIFGEITRAVVVDPHTIRFEFHQ
                     PNRELPLLAGGMPVFSRKWGMKPDGSRIPFDQLAFEKPIASGPYLIDQYDNGRTITYR
                     RDPHYWGATLPVRVGMNNFDRIVYKLYSDSTARLEAFKAGEYDALVEYVARNWVRRDV
                     GKKFDSGELIKREFAQHNGTGMQGFVLNMRRPLFQDVRVRKALDLALDFQWLNRQLFF
                     NQYTRLDSFFANTDLQAKGLPSPGELALLEPWRARLDPAVFGVPPKQPDTDPPGSLRA
                     NLLQARALLQQAGWTYRDGALRNAKGEPFQFEILDDSGSSAQFEPIVATLIRNLQKLG
                     ITATFRVSDFAVYQKRLDAFDFDTTTIRMADVQVPGSEQIDRFGSKAADTPGSDNVIG
                     LKSPAVDAILSALVHAQTREQLIDATHALDRVLMHGYYVIPQWYSASHRVAFKRGLAW
                     PKTLPLYYGAEGWITSMWWYAQPQQPQAQPH"
     gene            8563..>9268
                     /gene="fabI"
                     /locus_tag="VHV99_10800"
     CDS             8563..>9268
                     /gene="fabI"
                     /locus_tag="VHV99_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006026016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEX3635961.1"
                     /translation="MGFLAGKRILLTGLLSNRSIAYGIAQACKREGAELAFTYVGERF
                     KERITEFATEFGSDQVFPCDVADDAQIDALFASLKTRWEGLDGLVHSIGFAPREAIAG
                     DFLDGMTRENFRIAHDISAYSFPALAKAAQSMLTPDASLLTLSYLGAERAIPNYNTMG
                     LAKASLEASVRYLAVSLGAKGVRVNGISAGPIRTLAASGIKGFGKILEFVEKNAPLKR
                     NVTIEQVGNVAAFLLSD"
BASE COUNT         1890 a         2907 c         2827 g         1644 t
ORIGIN      
        1 gcgaatgttg ccgaggtgga tgaagccggt gggactcggt gcgaaacggg tacggacgga
       61 ggtggtcatt agcggttact cggaagacag gcgccggcgc cgtggctggc atcgccagtg
      121 aagcgcggca gcggagcgcg cccgtgcagc gccaagcgcc ttgcacgaac acgaaggaaa
      181 tagatgcgag agccgaatta taccttccga acccgggtta cctgacctcg tccgaacggc
      241 caatggcagt gcacaaaacg cgcaagaagc atgcgccaga gcgcccgagg gcttcctctg
      301 caacgtttca ttaccgcgcc gccgcgcgcc cggcgctttg atttatgatg tgcgcgctac
      361 gggtagggat ggaaccacag cgcatggaat cgccatcgac ggccctggcc gtttgactgt
      421 ccacctgatc gcggcacagc ttgcggtcgc ctgcgctggt tgcgctggtt gcgctggttg
      481 cgctgactgc actgcttgcg ctgcttgcgc tgactgcact gcttgcactg cttgcactga
      541 ctgccccgct tgcactgctt gcactgactg ccccgcttgc accgattaca tcgcctctgc
      601 cgttgctgga gaacccgttg aaaccgtcac ccccccgcct tgcccgtcgc aacttctcat
      661 tggcggccgc ctccgcggtg ctggctgcct gcaccacgac cggcggcggc aaaaccgata
      721 gcacaccggt tgccgctacg cccaccacca acacgccgcc gctcgacaag ccgcagcgcc
      781 cgatccgcgt cgggctcgcg ctaggcggcg gcgccgcgcg cggcttcgcg cacatcggcg
      841 tgatcaaggc actggaggcg cgcaatatcc aggtcgacct ggtggccggc acgagcgcag
      901 gttcggtggt cggcgcgctg tacgcgtcgg ggatgaacgg ctttgcgctg aacaagctcg
      961 cgctgaccat ggacgaagcg tcgatcagcg actgggccat gccgtttcgc acgcgcggct
     1021 ttctgcaagg catcgcgttg cagaactacc tgaacacgac gctcaacaac cgtccgatcg
     1081 agaagatggt caagccgctc ggcgtggtcg ccaccgattt gaagaccggc cagccgatcc
     1141 tgttccagcg cggcaacacc ggcattgcgg tgcgcgcgtc atgcagcgtg ccgtcgattt
     1201 tcgagccggt gaagatcggc ggccacgaat atgtggatgg cggtctggtg agtccggtgc
     1261 ccgcctcgtt cgcacgcaaa atgggcgcgg atttcgtgat cgccgtggac atctcgcagc
     1321 gaccggaaag cggcctcacc gccagttcgt tcgatgtgct gatgcagacc ttcacgatca
     1381 tgggccagac gatcaaggcc tatgaactcg acaagtacgc cgacatcgtg atccggccga
     1441 atctggcggc catgagcggc agcgatttct cgcagcgcaa tgcagcgatc ctggcgggcg
     1501 aggaagccgt ggcgaagatg atgtcggagt tgcaacgcaa gctggccaca agccgtgccg
     1561 cggtataacc cgcctgggtc gtgccgatta caccgacccg cgtgcttcct tcagccactg
     1621 tcgtatggag cacgcggggc ggacacagac cgggggcgca gcgcgaaaga ttcgccggca
     1681 catcttcgcc aagcgcacaa acgcaaaaag cctgcttcga acgaagcagg cttttttttc
     1741 gcaccgaaac cacaggtgcg ggccgaatca gttactgatt gccgccgatc gtagcactca
     1801 cgcgcttgcg cagatcttca ccgtcctgat aggacgtctc gatccggcgt gcacccgtga
     1861 aacgcttttc ccaatagccg ctatccaggt cgtcgacgcg gatcgtgctg cccgtggaag
     1921 gcgaatgcac gaacttgttg tcgccgatgt agatcccaac gtgcgagaag gtacggcgca
     1981 tggtgttgaa gaacaccaga tcgcccggct tcaggtcgct cacgcgaact ttttcgccga
     2041 cgcggctcat ttcctcggcg cggcgcggca gcgccatacc gagcgtgtcc tggaacacgt
     2101 agcgaacgaa gccgctgcaa tcgaggcccg aatcgggcgt attaccgccc caccggtagc
     2161 gaacgccgat catgttcagc gcgccgacca ccacgtcgcc cgctttaccg gccatgccgg
     2221 aaaggaacga tttggcgccg cggtcgctgc tgggagcggc cgattgcgaa ttgagggggg
     2281 acgaggaatc cgacccgtta ggggtcgaat atgaggcatt ctgattaaaa ctgcttactt
     2341 cgtcggcgaa agcgccggga gctgctgcca tcaagacgcc aatgaacatc ccggcgacga
     2401 cgcgcgtgca agcctgggtt aggtttctgt gctgcattgg tcggtatttt ttgcctaaaa
     2461 atcagtggac tacggaaaaa agtttggtcg atactagcca gtaagtattc acatgtcaaa
     2521 acaaatagaa aaatacaatc gagacacgca ctcaattggt gaaagagcgc ggaatcaacg
     2581 atccagcgcg gctttcagca gctttcgagt gtaagcgtgc accggtgtcg caaaaatctg
     2641 ctcaacattt ccgctttcga cgatcgaacc gttttgcatc accgccaccc ggtgcgccat
     2701 cgctccgatc acggccagat cgtggctgat gaaaacgaag cccaagttgt acttctgctg
     2761 caaaccggcg agcagtttca gcacctgctg ctggatcgag acgtcaagcg cactggtcgg
     2821 ctcgtcgagg atcaggatgc gcggctcgag caccagcgcc cgcgcaatcg caatccgttg
     2881 acgctgaccg ccggagaact catgcggata gcggtacaac accgtgcggt cgagaccgac
     2941 ttcgcgcagc accgaaacca ccttgtcgcg ccgcgccgcc tcgctcattt gcggccgatg
     3001 cagcgcgagc ccttcgccga cgatccgctc gatagtctga cgtggagaaa gtgaactgaa
     3061 cggatcctga aagacgactt gcatgttcga gcgtaaggcg gtctgttcgg cgccacgata
     3121 gctgccgagc gccctgccct gaaactcgat ctcgccgtgc acggtgcgtt gcaaaccgag
     3181 cagggccatc gcgagcgtcg atttgcccga acccgattcg ccgacgatcc ccagcgtctc
     3241 gccctggcgc accgacacgt tcgcatcggc aaccgcgcgg aagcgccccg aacgaaacca
     3301 gccgctcacg cccggcagtt tcgttttgaa atcgaccgag acatcgcgcg cttcgagcag
     3361 caccggagaa atgggcagca ccggcaccac cgtgcgctgc ggacggctcg ccagcagccg
     3421 ctgcgtgtac ggatgttgcg gtgactcgaa cacctgctcg accgggccgc tttccacgag
     3481 tatgcccatc tccatgaccg cgatacgttg cgcaaagtgc cgcaccagat tcagatcgtg
     3541 ggtgatcagc agcacggcca tgccgcgttt ctcggcttcg tcgcgttgca gctccagcag
     3601 caaatcgacg atctgcgcgc ggatcgtcac gtcgagcgct gtggtcggct cgtcggccag
     3661 caacaggcgt ggacggcacg caagcgccat ggcgatcatc gcgcgctgcc gttgaccgcc
     3721 cgagagttga tgcggatagc tgttcacacg cttgccaggc tcggcgatgc ccgtgcgccc
     3781 cagcaatgcc acggcgcgct tacgcgcttc gttcgccgtg acgccatcgt gtatgacgat
     3841 cgtctccgca atctggtcgc cgaccgtata gagcgggttg agtgccgtca tcggctcctg
     3901 gaagatcatc gcgatgtccg agccgcgcat gccacgcatc tcgcgctcgc ttttggcgag
     3961 caaatcctgg ccgtcgaaac ggatcgcccc gctgacctgc gcgtcgctca acagacgcag
     4021 gattgacagc gccgtcacgc tcttgcccga gcccgattcg ccgactaatg cgacccgctc
     4081 gccgcgttgg atcgcgagcg tcacatcgtt cactgcgacg gtgtcaccga aagtgacatg
     4141 caagtggtcg agttccagaa gcggcggcgt catggcttgt tttacattgc tctccactgc
     4201 cgcgctcact ggttgccccc ggcacgcatc gcatcggaga tgcgggtatc gagcgcattg
     4261 cgcagcgcat cgcccatgaa cgtcagcagc aacaacatcg cgaccagcac gccgaaggtg
     4321 gacaacgaga tccaccacgc gtcgagattc gctttgcctt gcgcaagcag ttcgcccagg
     4381 ctcggcgtcg gcgacggcac gccgaggccg agaaagtcga ggctggtgag cgcgaggatc
     4441 gcaccgctca tgcgaaacgg cagaaacgtg atcacaggcg tcaaactgtt gggcaagaca
     4501 tgccgccaca tgatctgcca gttcgagagc cccatcgccc gtgccgcccg cacatagtcc
     4561 tggtgacggt tgcgcagaaa ctccgcgcgc acgtaatccg acaaaccgat ccagccgaac
     4621 aacgagagca gcacgatcaa cagaatgaac cccggctcga agatcgagga gaaaatgatc
     4681 aacaggtaaa gctccggcat ggcactccag atttcgatca gccgctgccc gatgatatcc
     4741 gtacgtcctc cgaagtaacc ctgcacagcg ccggcggcaa tcccgagtac ggtgccgatc
     4801 aaggtcagca cgagcgcgaa ttccaccgac acgcgaaaac cgtacagaag acgtgcgaaa
     4861 agatcgcgac cgcggtcgtc ggtgccgaac cagttatcgc gcgacggcgg cgccggattc
     4921 ggcgccttcg agaagtaatt cagcgtgtcg tagtaatagc gattcggcgg atataccgcg
     4981 aagttacccg gcgcgtcgaa acgatgcttg atatacgggt caagatagtc ggcgggggtc
     5041 gggaagtcac cgccgaacgt agtctccgca taggtcttga acatcggaaa atacaactgc
     5101 ccttcgtaac gcaccaccag cggcttgtca ttcgaccaga gcgggccgac gaggctcgcc
     5161 gcgaacgcga cgacgaagat gatcagactc caatagccca ggcgctgctg acgaaaacgc
     5221 tgccagacgc gacgcgcggg cgttggcgat acgaacgcgc gagccggttc ggtgcgcgcc
     5281 gatgcatcgg cggaaagacg ggctcgattc atcagcgctc cagttgttcg aattggatgc
     5341 ggggatcgac ccacacatag caaagatcgg aaatcagctt ggtcgcgaga ccgatcagcg
     5401 tgaacagata cagtgtgccg agcacgaccg gatagtcgcg ccgcaccacc gattcgtacg
     5461 acagcaggcc aagtccgtcg agcgaaaaca gcgtctcgat cagcaggctg ccggtgaaga
     5521 acgcaccgat aaacgccgcc ggaaaaccga cgatcaacgg cagtaacgcg ttgcggaaca
     5581 cgtgcttcca caacacgcgc ttctcggaca aacctttggc gcgtgcggtc agcacgtatt
     5641 gcttgcggat ttcatcaagg aaggcgtttt tcgtcagcat cgtgacgacc gcgaaactgc
     5701 ccaccaccga ggccgtgatc ggcaacgtga tatgccacag gtagtcgacg atcttgccgc
     5761 cgagactcaa ctgcgcccag ttgtccgagg tgagattgcg cagcgggaac agttgcagga
     5821 agctgccgcc gccgaacagc accagcaaca gcacgcccag cacgaagccc ggaatcgcgt
     5881 agccgatcag cacgacgagg ctcgtggcaa tgtcgaagcg cgacccgttg cgcactgctt
     5941 tggcgatacc cagtggcacc gatatcagat acgtcagaaa gaacgtccac aagccgatac
     6001 tgatcgacac cggcaacttc gaaacgatca gcgaccacac gctttgatgg cggaaatagc
     6061 tctgcccgag atcgaacgtc gagaagcgct ttagcatcag gacgtagcgt tcgagcggtg
     6121 gtttgtcgaa gccatagagc gctttcagtt gcgcgatttg ctgcgcgtcg acgccggtat
     6181 gcgcacgcag accgaacggc gcgccgttct ccgccccttt gcgcagctcg tgctgcatct
     6241 gttcgaccgg accacccggg acgaactgga tcacggcgaa agtcagcgtc agcacgccga
     6301 gcaaggtcgg gatcatcagc agcaagcgtt tgaggatgta gctccacatg gcgatgtttc
     6361 cggtgcgaag gcagtgaatg aatgggcgtt tttcagtgcg gctgtgcttg cggctgttgc
     6421 ggctgtgcat accaccacat cgacgtgatc cagccttccg cgccatagta cagaggcagc
     6481 gtcttcggcc acgcgagccc gcgcttgaac gccacccgat gcgaagcgct gtaccactgc
     6541 ggaatcacat agtagccatg catcagcacg cggtcgagcg catgcgtggc gtcgatcagt
     6601 tgctcgcgcg tctgcgcatg cacgagcgcg ctcaggatcg cgtcgacggc cggcgacttc
     6661 agcccgatca cgttgtccga gccgggcgta tcagcagcct tgctgccgaa ccggtcgatc
     6721 tgctccgaac cgggcacctg cacgtccgcc atacgaatcg tggtcgtgtc gaaatcgaat
     6781 gcgtcgagcc gcttctgata gacggcgaag tcggacacgc gaaacgtcgc ggtgatgccg
     6841 agcttttgca gattgcggat gagggtcgcg acgatcggct cgaactgagc ggacgagccg
     6901 gaatcgtcga gaatctcgaa ctggaacggc tcgcctttgg cgttacgcaa tgcgccgtcg
     6961 cgataggtcc accctgcctg ctgcaataac gcgcgcgcct gcaacaaatt ggcgcgcagc
     7021 gagccgggcg gatcggtgtc cggctgcttc ggcggcacac cgaacacggc cggatcgagc
     7081 ctggcgcgcc atggctcgag cagcgccagt tcgcccggcg atggcaggcc cttcgcctgc
     7141 aaatccgtat tggcgaagaa gctgtcgaga cgcgtgtact gattgaagaa caactggcgg
     7201 ttgagccact ggaaatcgag tgcgagatcg agcgccttgc gcacacgcac atcctggaac
     7261 aatggccgcc gcatgttgag cacgaagcct tgcatgccgg tgccgttatg ttgcgcaaac
     7321 tcgcgcttga tcagctcgcc gctatcgaac ttcttgccga cgtcgcgccg cacccaattg
     7381 cgcgcgacat attcgaccag tgcgtcgtat tcgcccgcct tgaacgcctc gagccgcgcc
     7441 gtgctatccg aatacagctt gtagacgatc cggtcgaaat tgttcatgcc gacccgcacc
     7501 ggcagcgtcg caccccagta gtgcggatca cgccgatacg taatcgtgcg gccattgtcg
     7561 tactgatcga tcagatacgg cccgctggcg atcggcttct cgaacgccaa ttggtcgaac
     7621 ggaatccggc tgccgtcggg cttcatcccc cacttgcgtg agaacaccgg cataccgccc
     7681 gccagcagcg gcaattcgcg attgggttga tgaaactcga agcggatcgt atgcggatcg
     7741 accaccaccg cgcgcgtgat ctcgccgaag atcgatgcga attgcggcgc ggcttgcggg
     7801 cttttcagcg tgtcgagcga aaacttgacg tcgtccgcgg tgaccggatc gccattcgaa
     7861 aagcgcgcgc gcggattgat gtggaaggtg gtcgacaggc cgtcgggcgc gatactgatg
     7921 tcgtcggcaa gcaggccgta ggcggacgcc acttcgtcgc tgctgccggt ggtcaggctt
     7981 tcgaacagca gatccacacc tggggcggta ttgccgcgca acgtgaacgg attgaattta
     8041 tcgaagctgg tgagccggct cggattggcc atcaccagcg tgccgccctt cggcgcatcc
     8101 ggattgacgt agtcgaagtg cttgaagtcg gccggatatt tcggttcgcc gtattgcgcg
     8161 atcgcgtaga ccgcgtgggc ctcgggcgcg gcgagcaaac cggcgagagc cgacacgccg
     8221 cacaacgcgg ccaggcaggc cgagcgccag gaacgcggca caagccgcag tacgaccggt
     8281 acaccgacac gcgatcgcct gtgaagcacg ctgcacccgg gtgcacgagg cgtttgaacc
     8341 catcgcgagc cagttgtcat aggggccttg ttggtcagtg ggcaattgca atgtgcgaga
     8401 attctaccca ataaactcgc gccgtccgca catctcatcc gcatcaaccc tagcgcgtct
     8461 atcccgcgaa gcgagtgccg atgcgatggc tttcgcgcgg ccgcgttccc gggcaacgtc
     8521 acgacgtccg tgcgtgatgc gcagacctca tggggagaat tcatgggctt cctcgctggc
     8581 aaacgaatcc tgctcaccgg cctgttgtcg aaccgttcga ttgcgtacgg catcgcgcaa
     8641 gcctgcaagc gtgaaggcgc cgaactggcg ttcacctatg tcggcgagcg tttcaaggag
     8701 cgcatcaccg agttcgccac cgagttcggc agtgaccagg tgttcccatg cgacgttgcg
     8761 gacgatgcgc agatcgacgc cctcttcgcc tcgctaaaga cgcgctggga agggctcgac
     8821 gggctcgtcc attcgatcgg ttttgcgccg cgcgaagcca ttgccggcga cttcctcgac
     8881 ggcatgacgc gcgagaactt ccgcattgcg cacgacattt ccgcgtacag ctttccggca
     8941 ctcgccaaag ccgcgcaatc gatgctcacg ccggacgcgt cgctgctcac gctgagctac
     9001 ctcggcgccg aacgcgcgat tccgaactac aacaccatgg gtctcgccaa ggcctcgctc
     9061 gaagcgagcg tgcgctatct ggcggtgtcg ctcggtgcga agggcgtgcg cgtgaacggc
     9121 atttcggcgg gtccgatcag gacgctggct gcgagcggca tcaaaggctt cggcaagatt
     9181 ctcgaattcg tcgaaaagaa cgcgccgctc aagcgcaacg tgacgatcga acaggtcggc
     9241 aatgtcgcgg cgttcctgct gtcggatc
//
LOCUS       DATASH010000387         2321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1245421,
            whole genome shotgun sequence.
ACCESSION   DATASH010000387 DATASH010000000
VERSION     DATASH010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1245421"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="VHV99_10805"
     CDS             complement(<1..675)
                     /locus_tag="VHV99_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018418508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEX3635962.1"
                     /translation="MVKAAVLDRLWTRMSERGDFPMLSQSLRTSMAAVNNDDLDFTGL
                     VQVVLSDFALTQKVLRLANSAMYMAFGGNITTVSRALMVLGMDAVGHLVVGLKIVDHF
                     HQSAPRRIDAKLELNRTLLSGCVARKLTERGDLRAGEEAVVCTLMRQIGKLLVVFYLD
                     AEWDQIRRHIEDGTIEADACVLVLGVTFDEIGAEAAVRWRLPDMIRAGMGEFDPLDVE
                     QPRQVQW"
     gene            complement(767..>2321)
                     /locus_tag="VHV99_10810"
     CDS             complement(767..>2321)
                     /locus_tag="VHV99_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HEX3635963.1"
                     /translation="VAPADVARLDALLKRATASGNVEKATLGLIKSLTYPITVELRVV
                     RSSHDGIDSYAVAGFDVSAWRATEERLTQALHLDRLTNLANQPALIPALLQAQQQADA
                     HGTPAALLLLDLDDYQRVNRALGYDAGDEMLRDTARRLLNMTSPSETVARVASDEFAI
                     LIKPAASRTDAAAAAEALARRLLTAIQQPYVFNGQQVHLSASIGIALYPDVRHANDNA
                     AHDTHLLRWADHALLQAKAAGGNTLAFYVPDDNPADAERLKLEADLYDGVRNGEFSLH
                     FQPITSSRTHGVVGVEALIRWSHPVHGLVPPSMFIPLAESIGLINFLGNWVLKVACMQ
                     LIQWDARGISLQYVAVNVSPQQFRDPRFKDSVREAIELTGIDPRRLVFEITESLLMHD
                     PAHAKVLLEDLTAMGIRFAVDDFGTGYSSLAYLQRFPLAKLKIDRSFVENLLTSRNDQ
                     AIVSAVVGLAQTLDLELVAEGVETEAQRTLLTEMGCDHIQGWLVCKALPSDELAQRFE
                     ARTLHLHTA"
BASE COUNT          399 a          729 c          774 g          419 t
ORIGIN      
        1 ccactgcacc tggcgcggct gctcgacatc cagcggatcg aactcgccca tgccggcacg
       61 gatcatgtcg ggcaagcgcc agcgcaccgc ggcttcggcg ccgatctcgt cgaacgtgac
      121 gccgagcacc agcacgcagg catcggcctc gatggtgccg tcttcgatat gccggcggat
      181 ctgatcccac tcggcgtcga gataaaacac caccagcagc ttgccgatct gccgcatcag
      241 cgtgcagacc acggcttctt cgccggcacg cagatcgccg cgctcggtca gcttgcgcgc
      301 gacgcagccg gacagcagcg tgcggttcaa ttcaagtttc gcgtcgatgc ggcgcggcgc
      361 gctctgatga aagtgatcga cgattttcag gccgaccacc agatggccga ccgcgtccat
      421 gccgagtacc atcagcgcgc gcgacaccgt agtgatattg ccgccgaacg ccatgtacat
      481 ggccgagttg gcgagccgca acaccttttg cgtcaaggcg aaatccgaca gcacgacctg
      541 cacgaggccg gtgaagtcga ggtcgtcgtt gttcactgcc gccatggagg tgcgcagcga
      601 ttgcgacagc atggggaaat cgccgcgctc gctcatacgc gtccaaagtc ggtcgagcac
      661 cgccgccttt accatgtgag tcccgcaaaa accatacatg ttccattgaa tgccacgccg
      721 gcatcgagcc ggtatcttcc gctgcgcacg aggtttgcct gcatcgctat gcggtgtgca
      781 ggtgaagcgt gcgtgcttcg aaccgctgcg cgagttcgtc ggagggcagc gccttgcaga
      841 cgagccaccc ctgaatatgg tcgcatccca tctcggtgag caaggtgcgt tgtgcttctg
      901 tttcgacgcc ctcggcaacc agttcgagat cgagtgtttg cgccagcccg acgactgcgc
      961 taacgattgc ctgatcgttc cgggaggtta gcagattctc gacaaaactc ctgtcgatct
     1021 tgagcttggc caaggggaat cgttgcaggt aagccagact cgaataaccg gtgccgaaat
     1081 cgtcgacggc aaagcggatg cccatcgccg tcaggtcttc gagcagcacc ttggcgtgtg
     1141 ccgggtcatg catcagcagg ctttcggtga tctcgaacac gaggcggcgc gggtcgatgc
     1201 ccgtcaactc gatcgcttcg cgcacgctgt ccttgaagcg cggatcgcga aactgctgcg
     1261 gcgagacgtt gacggcgacg tattgcaatg agatgccccg cgcgtcccac tggatcagtt
     1321 gcatgcaggc gaccttcaac acccagttgc cgaggaagtt gatcaggccg atcgattccg
     1381 cgagcggaat gaacatcgaa gggggaacga ggccgtgcac gggatgcgac cagcggatca
     1441 acgcttcgac accgaccacg ccgtgcgtgc ggctgctcgt gatcggctgg aagtgcagcg
     1501 agaactcgcc gttgcgcacg ccgtcgtaca ggtccgcctc gagcttcagg cgctcggcgt
     1561 cggcggggtt gtcgtccggt acgtagaagg cgagcgtgtt gccgccggcc gccttggcct
     1621 gcagcaacgc atgatcggcc cagcgcagca ggtgggtatc gtgggcggcg ttgtcgttgg
     1681 cgtggcgcac gtcgggatac agcgcgatgc cgatgctcgc cgacagatgc acctgctggc
     1741 cgttgaatac atacggttgc tggatcgcgg tcaacaggcg gcgggccagc gcttcggccg
     1801 cggcggctgc gtcggtgcgg ctcgcggctg gcttgatcag gatcgcgaac tcgtcgctgg
     1861 cgacccgcgc caccgtctcg ctcgggctcg tcatgttcag gaggcgccgc gcggtgtcgc
     1921 gcaacatctc gtcgccggca tcgtagccga gcgcgcggtt cacgcgctga tagtcgtcga
     1981 ggtcgagcag cagcagcgcg gccggggtgc cgtgggcgtc ggcttgctgt tgcgcctgga
     2041 gcaacgcggg gatcagcgcc ggctgattgg ccagattggt caggcggtcc agatgcagcg
     2101 cctgggtcag gcgttcctcg gtggcgcgcc aggccgatac gtcgaagccg gcgaccgcat
     2161 agctgtcgat gccgtcgtgg ctgcttcgca ccacgcgcaa ctcgactgtg atcgggtagg
     2221 tcagcgactt gatgaggccg agcgtggcct tttcgacgtt gcccgaggcg gtggcgcgct
     2281 tcagcagcgc gtcgaggcgc gccacgtcgg cgggcgccac g
//
LOCUS       DATASH010000388        11491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4411945,
            whole genome shotgun sequence.
ACCESSION   DATASH010000388 DATASH010000000
VERSION     DATASH010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 11491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11491
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4411945"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..1092)
                     /gene="egtD"
                     /locus_tag="VHV99_10815"
     CDS             complement(109..1092)
                     /gene="egtD"
                     /locus_tag="VHV99_10815"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEX3635964.1"
                     /translation="MTQPALSHDASPDLLRATFAADVRAGLTHTPQKELPSKYLYDEV
                     GSALFEVITVLPEYGVTRAEEHLLTKHAADIVAHLPHDVTVAELGSGSGRKTRRILEA
                     LCKKRPTSYCPIEISRSALQLCRRELGDIERISIVGYERDYLAGLAEVSKNRAADERL
                     LVLFLGSTIGNFGRLAATRFLREIRNMLAPGDALLLGTDLIKPTPVLVAAYDDSIGVT
                     ASFNLNLLARINRELDGDFPLDAFEHVARFNPDARSIEMHLRAKRDLTAHVGAAQLKV
                     SLKAGETIWTESSHKYRADEMPAIADDAGFVCSHQWIEDQWGFAESLLVAR"
     gene            1708..2604
                     /gene="rarD"
                     /locus_tag="VHV99_10820"
     CDS             1708..2604
                     /gene="rarD"
                     /locus_tag="VHV99_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018438375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HEX3635965.1"
                     /translation="MNQYDPKRGIALSVGASAMFALLSAYAKLLAPLSGLDIFAWRVV
                     WTVPGALLLILLRKRLPILRQLVYRMVTQPKLGVAMIVSAALLGVQLWVFLWAPLHGR
                     MLEVSLGYFLLPLVMVLVGRFYYHESLDGLQWLAVVCAALGVAHELWMTGAFSWPTLL
                     VALGYPPYFVLRRKINQDSLAMFTVEMALLLPAAAVFIVIGGSLTLIGGRIDMWCLLL
                     PGLGALSTVALASYLKASRLLPVALFGILGYVEPVLLVFVSITLLGEALSAEQLATYI
                     PIWIAVSLTALHSVRLVRFAAR"
     gene            complement(2745..3284)
                     /locus_tag="VHV99_10825"
     CDS             complement(2745..3284)
                     /locus_tag="VHV99_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mpo1-like protein"
                     /protein_id="HEX3635966.1"
                     /translation="MRTLTQQLTQYAAYHHDRRNIATHFIGIPMIVLALAVLLSRPAF
                     SVGGLSLILSPAWVLFAAATVYYLVLDVPLGVMMAVVSALCVACGQWLAAQATLTWLG
                     SGIGLFVIGWVFQFIGHVAYEHRKPAFVDDVIGLLIGPLFVLAEALFGFGWRPALREA
                     IEAQVGPTRINADSAAAHR"
     gene            3398..3544
                     /locus_tag="VHV99_10830"
     CDS             3398..3544
                     /locus_tag="VHV99_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635967.1"
                     /translation="MSLPRCWSAAYGFARRPLQGALKLGYAEIEIADVQKLSALADLN
                     PAAH"
     gene            3963..4331
                     /locus_tag="VHV99_10835"
     CDS             3963..4331
                     /locus_tag="VHV99_10835"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX3635968.1"
                     /translation="MKIARAVLIEADRQAISPILLLAVMSVESSFDRYAVSIAGARGL
                     MQILPAAHPQLIAGTTDLSDPAINVRIGSTILRDYLDESGGDLDAALLRYSGGGRGYA
                     RRVVLRMQRLDASLRHEMMN"
     gene            complement(4376..5260)
                     /locus_tag="VHV99_10840"
     CDS             complement(4376..5260)
                     /locus_tag="VHV99_10840"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEX3635969.1"
                     /translation="MFSGIWLPIVTPLRNGEVDVDALQRLADYYLRTEISGFVALSTT
                     GEAALLQESERLTVLQALTDVIGSRLPMLIGVGGSNTRDVLHDIQRYERWDCAGYLVS
                     PPSYVCPDQAGVQWHFEQAALATERPIVLYNVPHRIGVTIEPDTVARLVENSNIVAIK
                     ECVKEHFEKLRGLPVNVLCGTDEALDDCLNHGGTGGILASAHVCADLLVAVQELIRHA
                     HDMDARNLFASLLPVLRLLFAAPNPSAIKAMLAFDHSMTDETRMPITRASAQLVERLS
                     TARDRLQDLRAEFAGAMS"
     gene            complement(5263..5604)
                     /locus_tag="VHV99_10845"
     CDS             complement(5263..5604)
                     /locus_tag="VHV99_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635970.1"
                     /translation="MALTQSIDSFETQPLSAPPVQRNNVIPFPAARARLQVVLPHPPH
                     DLPGTPLRTLLHSPLGVYVVSTEADREEVRVQLDIALDDLDFTLHTLISTLPAALIGP
                     LKRRQAVAKAR"
     gene            complement(5902..6273)
                     /locus_tag="VHV99_10850"
     CDS             complement(5902..6273)
                     /locus_tag="VHV99_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635971.1"
                     /translation="MKTLFAALASALVVSTAFAQTAAPSAAQPAQTQPAGQANLKAST
                     AVQTGAADNGAATTKAPAKATAKAHVKKTSAAHTAKMHKATKKTAVEASAGKTKTESA
                     KEASNAPVKADGAKADTTKTQ"
     gene            complement(6520..6780)
                     /locus_tag="VHV99_10855"
     CDS             complement(6520..6780)
                     /locus_tag="VHV99_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635972.1"
                     /translation="MFKPYSNDDDVLNIQGDALTVSNGTTRVVLNGTLELTQDQRGLK
                     AALALKEAIDAVVAALQAQTNLPAKLKDEPDAKPGVVQNPFQ"
     gene            complement(6773..7522)
                     /locus_tag="VHV99_10860"
     CDS             complement(6773..7522)
                     /locus_tag="VHV99_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEX3635973.1"
                     /translation="MKKWLACLLFAVAAHASAAPSCTQFTPNAQWPVLTNQKMEPKTR
                     MLCYSDFAVLHSGITHGPLWSAEHLTRDHIEAAKDMVRTNKFFEDARLPDGEGATLAD
                     YKRSGFDRGHMSPAGNRWNQEAMAQSFSLANVVPQNRENNQRLWARIETSVRRIAMSY
                     DDTYVVTGPIFSGQQLQTIGPTHVFVPTQLFKVVYVPSRQMAFAVMVDNVSTNRYDIR
                     TIHELEAASGIRFPGIPENLKDQRPGGLKGV"
     gene            complement(7906..8265)
                     /locus_tag="VHV99_10865"
     CDS             complement(7906..8265)
                     /locus_tag="VHV99_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3564 domain-containing protein"
                     /protein_id="HEX3635974.1"
                     /translation="MRLTILINGSDPTVSHDYAVLWLDTEEHRWSREAHQGIDLPPLG
                     ELHDDNGVTTLCAPSTDAPLCTLRGLHVDRKQRVSAAQGAAAWTALPTRAPTSGFWRL
                     QAVDRQNVRAENSVFGN"
     gene            8425..9096
                     /locus_tag="VHV99_10870"
     CDS             8425..9096
                     /locus_tag="VHV99_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4410 domain-containing protein"
                     /protein_id="HEX3635975.1"
                     /translation="MLRRFLQLTMLAGCVGAATLSAAADSVPFATPKPIVYVSDFDLD
                     VANVTPDSGPGERARRLRGLLPGGPGPLGHDSNPQDHAKDIVNEMAEALTADLQKGGV
                     DARRSAPGQPLAATGWQVRGVFLSVDDGNRLRRAMVGFGTGQNDIQVAVSCDSLGAAD
                     LPPLYQAVEEADSKAKPGAVIKLNPYVIAAKFVMASGDEKKTIKKTAQEISDSVIAKL
                     HGVTQ"
     gene            complement(9184..10869)
                     /locus_tag="VHV99_10875"
     CDS             complement(9184..10869)
                     /locus_tag="VHV99_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase"
                     /protein_id="HEX3635976.1"
                     /translation="MTDNDSTLPPNPDNAPEDPERRRVLTGLAAVGLGLALAGCKTEQ
                     SAAGDTAQRSAADLRLDAALHDQIKHIVVIYAENRSFANLYGNFPGVQHPLDAVSGER
                     YLQLDRDGKTPLPRLPAIWGGLVPQAQEVDGKRYMIGEKDIRHLRNAPFHITDAQGAP
                     LPNGVITRDLVHRFYQNQMQINAGRNNQFAAWGDSGGLVMGHYRNSADSLRLWNLAQQ
                     YTLCDNFFMAAFGGSWLNHIFLISAQAPFYPDIHNSPAKKMVSVVEGDDPTGTRLKQA
                     TDSPASALGGPPKFVNDGAFTADGYAVNTMAPPYQPSNVRPAEGGNPAFADLSNPRVL
                     PPQNYATIGDRLTDKGVDWAWYSGAWQYALEHQDTGAVPDFQYHHQPFNYFTNYAPGT
                     AARRKYLRDAGLGDDASTNRLIADIDAGRLPAVTFYKPQGNLNMHAGYADVASGDRHI
                     ANVIEHIQRSPQWANTVVVVTVDENGGWWDPVSPPKGDRWGPGSRIPALVVSPLAKKG
                     YVDHTVYDTNSILRLISRVHGLAPLDGVVARDRAFAQNGLAPLGDLTGSLDLA"
     gene            complement(10974..11261)
                     /locus_tag="VHV99_10880"
     CDS             complement(10974..11261)
                     /locus_tag="VHV99_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635977.1"
                     /translation="MSESIQIEVADSHLYPGCAVHIAHLPEPAHAVAAVVEFADGSGA
                     HATCHRRTFDELELTVDGYATQRRHPVDARHWLLLAVDATHNSWRVKRRLP"
BASE COUNT         2173 a         3350 c         3647 g         2321 t
ORIGIN      
        1 gccgttttgt cgattgtgtg tctcgcgtgt ccagcgcgcg gcccgataat gcttaccgct
       61 taccgcttac cgcttaccgc ttaccgctta ccgcttaccg cttaccgctt accgcgccac
      121 cagcaagctc tccgcgaagc cccattgatc ctcgatccac tgatggctgc acacgaagcc
      181 ggcgtcgtcg gcgatcgcgg gcatctcgtc ggcgcgatat ttgtggctgc tctcggtcca
      241 gatcgtttcg cctgccttga gcgagacctt caattgcgcg gcgcccacgt gcgcggtgag
      301 atcgcgcttc gcacgcagat gcatttcgat gctacgcgcg tccggattga agcgggcgac
      361 gtgttcgaac gcatcgagcg gaaagtcgcc gtcgagttcg cgattgatgc gcgccagcaa
      421 attcagattg aacgatgccg tgacaccgat tgaatcgtcg taggcggcca cgagcaccgg
      481 cgtcggcttg atcagatccg tgccgagcaa cagcgcgtcg ccgggcgcga gcatattgcg
      541 gatctcgcgc agaaatcgcg tcgccgcgag cctgccgaaa ttaccgatcg tactgccaag
      601 aaacaggacg agcagccgtt cgtccgccgc gcggttcttg ctcacttcgg ccagtccggc
      661 caggtaatcg cgctcgtaac ccacaatcga gatccgctcg atgtcgccga gttcgcgcct
      721 gcacaactgc aaggcgctgc gcgaaatttc aatcggacag taggaagtgg ggcgcttttt
      781 acacagcgct tccagaatgc gccgcgtttt gcggccgctg ccgctgccca gttcggcgac
      841 ggtcacgtca tgcggcagat gcgcgacgat gtccgctgcg tgcttcgtaa gcagatgttc
      901 ctccgcgcgt gtcacgccat attcgggcag cacggtgatc acttcgaaca gcgcggagcc
      961 gacttcgtca tacagatact tcgagggcag ctctttctgc ggcgtgtggg tgagtccggc
     1021 gcgaacgtcg gcggcgaagg tggcgcgtag tagatcgggc gatgcgtcgt gcgagagggc
     1081 tggctgggtc atgctggctc cagtagtcac attcgatgag caggattgct ggccgggtgg
     1141 cggcaggcgc actgaagcgc aggtgatgca tgccgcggga aggaaaggcg ggctcggtcc
     1201 gcatggactg ccttgccggc ccggctgatt gatgttgccg gtgacgcttg caaatgacgc
     1261 tgcaatggcg gttgcgacgg atgtccaggg tgtcctgaat gacgttgcaa acggtgtcgc
     1321 cagttttcgt agcaagattc gcgcttgaac atctgcgcgg gaagtggcgg gcgttgccgc
     1381 tgcggcttcg cccgtcgcgc gtgaagttat gttctagtgc atcggcgcgc gatgcgcacg
     1441 gaataaattg ctgctcgctg taacgaactc gcgacgaacc cgccggcaca tttgcgccgg
     1501 aacccgtcta gaatgcccgc ttgcatcacc tgtcgaagat aaaaaccgca gcgcttgatc
     1561 tgcgctgcct gcttgtgaga cttcacttct aatgacaaca actcaaggcg cgcagccgca
     1621 tgttgcgccg cacacacttg catcaacatc agtactggct cacccgtctg cgccgcgtgc
     1681 cgaaacgact gcgtcagggg ccgcgtaatg aaccagtatg acccaaagcg cggtatcgcg
     1741 ttgtccgtcg gcgcgtcggc gatgtttgca ttactgtccg cctacgccaa gctgcttgcg
     1801 ccgctgagcg ggctcgacat tttcgcgtgg cgcgtcgtgt ggaccgtgcc gggcgcactc
     1861 ttgctgatcc tgttgcgcaa acgcctgccg attctccggc aacttgttta ccgcatggtg
     1921 acgcagccga agctcggcgt ggcgatgatt gtcagcgcgg ctttgcttgg cgtgcaactg
     1981 tgggtgtttc tatgggcgcc gctgcatggg cgcatgcttg aagtctcgct cggctatttt
     2041 ctgctgccgc tggtgatggt gctggtggga cgtttctact atcacgagag cctcgacggt
     2101 ctgcagtggc tagccgttgt ctgcgctgcg ttgggcgtcg cgcatgaact gtggatgacg
     2161 ggtgcctttt catggcccac gttgctcgtc gcgctcggtt atcccccgta cttcgtgctg
     2221 cggcgcaaga tcaaccagga ctcgctggcg atgttcacgg tggaaatggc gctgctgctg
     2281 ccggcggcgg ccgtgtttat cgtcatcggc ggttcgctga ctttgatcgg cggccgtatc
     2341 gacatgtggt gtttgctgtt gccggggctc ggcgcgctca gcaccgttgc gctggcttcg
     2401 tatctgaagg cgagccgctt attgccggtc gccttgttcg gcattctcgg ctatgtcgag
     2461 ccggtgctgc tggtgtttgt ctccatcacg ttgctcggtg aggcgctgag cgccgagcag
     2521 ttagccacgt atattcctat ctggattgcg gtctcgctga cggcgttgca tagcgtgcgg
     2581 ctcgttcggt tcgcggcgcg ttgagccgcg gtggtcgaga cacggcggcc ggtgagttga
     2641 gacggttgat tgagtcggga tggccggttg aattgggacg gtcgatcgag tgtggatgac
     2701 ggcctgagtt tagcataggc tgccaaatcg ggcgccccta tttgtcaccg atgcgccgcc
     2761 gcgctgtccg cattgatacg ggtcgggccg acctgtgcct cgatcgcttc ccgcagtgcg
     2821 ggccgccagc caaaaccaaa caacgcctca gcgaggacga acagcggccc gatcagcaga
     2881 ccgatcacat cgtctacaaa ggcaggcttg cgatgttcgt atgccacgtg gccgatgaat
     2941 tgaaagaccc agccgatcac aaagaggcca atacccgagc cgagccaggt gagcgtagct
     3001 tgcgcggcga gccactgccc gcaagcgacg cacaacgccg acaccacggc catcatcacg
     3061 ccgagcggca catcgagcac gagatagtag acggtcgccg cggcgaacaa tacccatgcg
     3121 ggcgataaga tcaacgacaa gccacccacg ctgaaagcgg gccggctcaa caacacagcc
     3181 agcgccagca cgatcatcgg aatgccgatg aaatgcgtgg cgatattgcg ccggtcgtga
     3241 tggtaggccg cgtattgcgt cagctgttgt gtcagcgttc tcatggatgc cgctcctcct
     3301 tcaaagtcag cataatcgcg ggcagcggaa tttgaaacca tcgggtagcc gacaatccga
     3361 cacggatgcc gtcctatgac tgcgagtatt tcatcgaatg agcttaccgc gttgctggag
     3421 cgcagcgtat ggtttcgctc ggcgcccgct gcagggcgcg ttgaagctgg gctatgcgga
     3481 aatagaaatc gccgacgtgc aaaagctcag cgcactagcc gatctgaatc cagcggcgca
     3541 ttgatcgaac tggattcccg gcaagctacg caaaagatgc ctgtacgtcc aagtcgcgtg
     3601 ccgcgcgatg cgatatttta taagcatttt acagcgcacc cctctttctt tggcgattcg
     3661 ctacaccaag cccggtaagg tcgagacacc cccggtcagg aagcgaacat atgcatggaa
     3721 atagcgctct ggatggtgcg caaccacaac gaaactttcg cctgagagtc ggcctgaagg
     3781 tgatgctggc agcgatcgtc ctttcgctgt ccgcggcatc gcaagccgtt gaggcccagt
     3841 cggttttgac cgcacaaacg ccgattgccg cgagcgacgc aacatctgac agcggcttgc
     3901 cggcgctcag cgatatcact gccttactgc gcgcacagtt tcgcgttact ccgacggaat
     3961 cgatgaagat cgcacgcgcg gtgctgatcg aagcagaccg ccaagccatc tcgccgatct
     4021 tgctgcttgc cgtgatgtcg gtcgagtcga gcttcgatcg ctacgcggtc agcattgcgg
     4081 gtgcgcgagg actcatgcag atcttgcctg ctgcgcaccc gcaattgatt gcgggcacaa
     4141 ccgacttgtc cgatccggcg atcaatgtgc gaattggctc gaccattttg cgcgactacc
     4201 tcgatgaaag cggcggcgat ctcgacgccg cgctgcttcg ttacagcggc ggcggccgcg
     4261 gttacgcgcg gcgtgtcgtg ctgcggatgc agcggctgga cgcaagcttg cgccacgaaa
     4321 tgatgaactg attcgtggcg cattcgtctg caaacctgct ccgctgctcc tcgcgttagc
     4381 tcatcgctcc agcaaattcg gcgcgcaggt cctgcaacct gtcgcgcgcg gtgctcaggc
     4441 gctcgacgag ttgtgccgac gcgcgcgtga tcggcatacg cgtttcgtca gtcatcgagt
     4501 gatcgaacgc cagcatcgcc ttgatcgcgg acgggttggg cgccgcgaac agcaaacgca
     4561 gaacgggcag caaactggcg aacagattgc gggcgtccat atcgtgtgcg tgccggatca
     4621 attcctgtac ggcaaccagc agatccgcgc agacatgtgc gcttgccaga atgccgccag
     4681 tgccgccgtg gttcaggcag tcgtccagag cttcatcggt gccgcacagc acgttgaccg
     4741 gcaggccacg cagcttctcg aagtgttcct tcacgcattc cttgatcgcg acgatgttgc
     4801 tgttttcgac cagacgcgcg accgtgtccg gctcgatggt gacgccgatc cgatgcggca
     4861 cgttgtacaa aacgataggc cgctcggtgg caagtgcggc ttgttcgaaa tgccactgca
     4921 cgccagcctg atccggacat acgtacgacg gaggagacac gagataaccc gcgcagtccc
     4981 agcgttcata gcgctgaata tcgtgcagga cgtcgcgcgt attcgagccg ccgacgccga
     5041 tcagcatcgg caggcgggag ccgatcacat cggtcagcgc ctgcaacacg gtaagacgct
     5101 cggattcctg taataacgcg gcttcgcccg tcgtgctcaa ggcgacaaaa ccgctgatct
     5161 ccgtgcgcag atagtagtcc gcgaggcgct gcaacgcgtc gacgtcgact tcgccattgc
     5221 gcaacggtgt gacgatgggc aaccagatgc ctgagaacat gattagcgcg cctttgcgac
     5281 ggcttggcgg cgcttcagag gtccgatcag tgctgcgggg agtgtggaga tgagcgtatg
     5341 gagcgtgaaa tcgaggtcgt cgagcgcgat gtcgagttgc acgcgcactt cttcacgatc
     5401 cgcttccgtg ctgacgacat acacaccgag cggcgaatga agcagtgtgc gtaacggggt
     5461 gcctggaaga tcgtgcggcg ggtggggcaa gactacctgg aggcgggcgc gggccgcggg
     5521 gaagggaatg acattgttgc gctggaccgg cggcgcgcta agcggctgcg tttcgaaact
     5581 gtcgattgat tgcgtgagag ccatttgatg cggcgttcgt tgacgccatg gttgatcaag
     5641 acacttcgag attagacgtc tacgggtaaa cgcagtgcaa aaatttcggt gtctgctgaa
     5701 aaaatcatgg ggactcgacg aaccgttcga attgaatcag gcagccacgc tggcaaataa
     5761 actttgcgtg cgcttgagcc agggtccatg cggcgcgcct ataaaaaaag gcgagtcgtg
     5821 agactcgccc tccctgaacg gcagttcggc ttcgattaac tacgcatgcg tggccggggc
     5881 ttgccgaaaa ccgatcacgt gttattgcgt tttcgtcgtg tcggcctttg cgccgtcggc
     5941 ttttaccggg gcattgctgg cctctttcgc gctctccgtt ttggtcttgc ctgcgcttgc
     6001 ttcgacagcg gtctttttcg tggctttatg catctttgcc gtgtgagcgg cggacgtctt
     6061 tttcacatgc gccttcgcgg tggctttggc cggcgctttt gtcgttgccg cgccgttgtc
     6121 cgcggcgccg gtttgcacgg cggtgctagc cttcagattc gcttgaccgg cgggttgcgt
     6181 ttgcgccggt tgcgcagccg acggcgctgc ggtttgtgca aaagcggtcg acacgacgag
     6241 agcggaagcg agagcggcga aaagcgtctt catggtgtaa ctcctggttt gaccggttat
     6301 ttcttgtgtc gcggctgaaa tgtttcagcg ctgccggttt aacgttggcc ggaagacagc
     6361 agttgacgat gctctggtga caaaacgtaa cggcggatac atatcggggt tctgaataaa
     6421 tcgcgagcga cggcaagata cgtacggcgg cgtcagcggc gttgaacagc tgactcgttg
     6481 cgatgcaatg ttggcacgcc tgaccgaata cgtctggcgc tattgaaacg gattctgtac
     6541 gacgccgggc ttcgcgtccg gctcgtcctt cagcttcgcc ggcaaattgg tttgcgcttg
     6601 caatgcggcc accaccgcgt cgatcgcttc cttcagcgcc agtgccgctt tcagtccccg
     6661 ctggtcctga gtcaattcca gcgttccgtt cagcaccacg cgcgtggtgc cattgctgac
     6721 ggtgagtgcg tcgccctgga tgttgagcac gtcgtcatcg tttgaatagg gtttaaacac
     6781 ctttcagtcc tccgggtcgc tgatctttga gattctccgg aatacccgga aaacggatac
     6841 cggaggccgc ctcgagttcg tgaatcgtcc tgatgtcata gcggttggtc gagacgttgt
     6901 ccaccatcac cgcgaacgcc atttgccggg acggcacgta gaccaccttg aacaattgtg
     6961 tcggcacgaa gacatgcgtc ggtccgatgg tctgcaattg ttggccgctg aagatcggtc
     7021 cggtgacgac ataggtatcg tcgtacgaca tcgcgatccg gcgcaccgac gtttcgatgc
     7081 gcgcccacaa gcgctgattg ttctcgcggt tctggggcac gacgttcgcc agcgaaaacg
     7141 attgtgccat cgcttcctga ttccagcgat tgcccgccgg gctcatatgc ccgcgatcga
     7201 agccgctgcg tttgtagtcg gcgagcgttg cgccttcacc gtcgggcaaa cgcgcatctt
     7261 cgaagaattt gttggtgcgc accatgtctt tggcggcttc gatatggtcg cgcgtcagat
     7321 gctcagcgga ccacagcgga ccatgcgtaa tacctgaatg cagcaccgcg aaatcgctat
     7381 agcaaagcat gcgcgttttc ggctccatct tctgattggt cagaaccggc cattgcgcgt
     7441 tcggggtgaa ctgggtgcag gaaggcgccg cgcttgcgtg agcggcaaca gcgaagagca
     7501 gacaggcaag ccacttcttc atgtgtcagt cagtcgaggg atcgggggcg caagtatagc
     7561 gtcgcgagcg cctatcgagc gacgcggaac gcaggaaaac acagaggaag aaagtggatt
     7621 ttttgcgcgg ggcgcggtga tgaaaatgat gcgggcgcag gaagcgcagg aagcgcaggg
     7681 ccgcaaagaa gaaatccaaa agcgccacac caaaaaaaac gggccgcttt gcagcgaccc
     7741 taatgcgtgg aattcagact ctactcatgc aaacttaaga ccggcttaaa tgcgcaacgc
     7801 cgaagctaga gggcggtaac gatcaaacac gcgcgcgaac acacccgcac gtgggccgtg
     7861 cggctcggtg agtaaaaatt tccccggcga aaaaagcgca ggaatctaat tgccgaaaac
     7921 gctgttctca gcgcgcacat tctgccggtc gacggcttgc aagcgccaga agccgctggt
     7981 cggtgcgcgc gtgggcaaag ctgtccacgc tgcggcgcct tgcgccgcgc tgacgcgttg
     8041 tttgcgatcg acgtgcaagc cgcgcagcgt gcacagcggc gcatcggtgc tcggcgcgca
     8101 cagcgtggtc acgccattgt catcgtgcaa ttcgcccaac gggggcagat cgatcccttg
     8161 atgcgcttcc ctcgaccacc ggtgttcttc ggtgtcgagc catagcacag cgtaatcgtg
     8221 gctgacggtg ggatccgaac cgttgatgag gatagttagg cgcatggcag ttccgtacaa
     8281 attgcgaagt ctagctttat cagtctatgc cgcaacgcgt gttatgcaag gcaaaggtac
     8341 gacgatcagg cacgaaaaaa acttacgcac cgtccatacg gcggtacgat agccgcgtct
     8401 atcagccatt cgcggaggcg cgccatgctg cgtagatttc tgcaactcac gatgctggcg
     8461 ggatgcgtcg gcgcagccac gctgagtgcc gcggcagaca gcgtcccttt cgccacgccc
     8521 aaaccgattg tctatgtatc cgacttcgat ctcgatgtgg ccaacgtgac acctgacagc
     8581 ggacccggcg aacgcgcgcg ccgtctgcgc ggtttgctgc cgggcggtcc aggaccgttg
     8641 gggcacgatt cgaatccgca ggaccacgcc aaagacatcg tcaacgagat ggcggaggca
     8701 ctgacggcgg atctgcagaa aggcggggtc gacgcacgcc gcagcgcgcc cgggcaaccg
     8761 ttggcggcaa cgggctggca agtgcgcggt gtgtttctga gtgtcgacga cggcaatcgt
     8821 ctaaggcgtg ccatggtggg ttttggcacg ggccagaacg atattcaggt ggcggtgtcg
     8881 tgcgacagcc ttggcgcggc ggatttgccg ccgctgtatc aggcggtcga agaggcggac
     8941 agcaaggcta agccgggggc ggtgatcaaa ctcaatccgt atgtgatcgc ggccaagttc
     9001 gtcatggcga gtggcgacga aaaaaagacg atcaagaaaa ccgctcagga gatttcggat
     9061 tcggtgattg cgaagctgca cggggtgacg cagtgattcg ctgcatcgtg cgaggtgaga
     9121 tgcctgcggt gccaccgcag gcatacagac acgccgccgc aaacctacgg caaagcgaac
     9181 cgcttatgcc agatccagcg aacccgtcaa atcgccgagc ggcgccaacc cgttctgcgc
     9241 aaacgcccga tctcgtgcca ccacgccatc caggggcgcc agcccatgca cgcggctgat
     9301 caagcgaaga atcgaattcg tgtcatacac cgtatgatcg acgtatcctt tcttcgccaa
     9361 cggcgaaacc accagggccg gaatccgcga ccctggcccc cagcgatcac cctttggcgg
     9421 tgagaccgga tcccaccagc cgccgttctc gtccacggtc acgaccacga cagtattggc
     9481 ccattgcggg ctgcgctgga tgtgctcgat cacattcgca atgtgccggt cgccggatgc
     9541 gacgtccgcg tatcccgcgt gcatgttcag attgccttgc ggcttgtaga aggtgacagc
     9601 cggcaaacgc cccgcgtcaa tatcggcaat caaacgattg gtcgacgcgt cgtcacccaa
     9661 acccgcgtcg cgcagatact tgcgccgcgc cgcggtgccc ggcgcgtagt tggtgaagta
     9721 gttgaacggc tgatggtggt attggaaatc gggcaccgcg cccgtgtcct gatgctccaa
     9781 cgcgtactgc catgccccgc tgtaccacgc ccagtcgaca cccttgtccg tgagacggtc
     9841 gccgatcgtc gcgtaatttt gcggcggcag cacgcgcgga ttcgacaggt cggcgaacgc
     9901 cggattgccg ccttcggccg ggcgcacatt gcttggctga tacggcggcg ccatcgtgtt
     9961 gacggcgtag ccgtcggcgg taaatgcgcc gtcgttgacg aatttcggcg gaccgccgag
    10021 cgcggatgcc ggcgagtcgg tcgcctgttt caagcgcgtg ccggtcggat cgtcaccttc
    10081 gacgaccgag accatcttct tcgccggact gttgtggatg tcgggataga acggcgcttg
    10141 cgccgagatc aggaagatgt ggttcagcca ggaaccgccg aaggccgcca tgaagaagtt
    10201 gtcgcacagc gtgtattgct gcgcgagatt ccacagacgc agcgaatcgg cggaattccg
    10261 ataatgtccc atcacgagac cgccggaatc gccccatgcg gcgaactggt tgttgcgccc
    10321 ggcgttgatt tgcatctggt tctgatagaa gcgatgcacg agatcgcgcg tgatcacgcc
    10381 attcggcagc ggcgcgcctt gcgcgtcggt gatatggaag ggcgcgttgc gcaggtggcg
    10441 gatatctttt tcaccaatca tgtagcgctt gccgtccacc tcctgcgctt gcggtacgag
    10501 gccgccccag attgccggca ggcgcggcaa cggcgtcttg ccgtcgcggt cgagttgtag
    10561 ataacgctcg ccgctcaccg catcgagtgg atgctggacg cccggaaagt tgccgtacag
    10621 gttcgcgaag ctgcggttct cggcgtagat caccacgatg tgtttgattt gatcgtgcaa
    10681 cgcggcgtcg aggcgtaagt cggcggcgct gcgctgcgcc gtgtcgcctg cggcactttg
    10741 ctcggtcttg caaccggcca gcgcgagtcc gaggccgacg gcagcgagcc cggtcaggac
    10801 gcggcggcgc tcggggtctt cgggggcgtt gtcggggttt ggcggcagag tggagtcgtt
    10861 gtctgtcatc ggtaggtttc gaggttagcg tgacgggcag tctggttgcc gctcggggcg
    10921 gcgagtgcgc ggcaccgggt tcggatacga agtgatcaga aagcttggtg aagctaaggt
    10981 agtcgtcgct tcacgcgcca gctgttgtgc gttgcgtcga cggcaagcag aagccaatgc
    11041 ctggcgtcga cgggatggcg ccgctgggtt gcgtaaccgt cgacggttaa ttcgagttcg
    11101 tcgaacgtgc gccgatggca agtggcatgc gcaccggagc cgtccgcgaa ttcgactacc
    11161 gccgccaccg cgtgagccgg ctcgggcaga tgcgcgatat gcactgcgca gcccggataa
    11221 agatgcgagt cggcaacctc gatctggatg gattcggaca tgggtgtggc ctccggacgc
    11281 atggcggcgg ccaaagcgcg gcaatgagac cttgcgcgat ggaccggatc aaaggatagc
    11341 accgcgggaa acgaaaatga gcgctgcgtg ctttgcaccc cacaccacgc aaggcgcggc
    11401 cgactcgcga gccaatcgcg ccactcccga tgcgtaaaag cggaggagag tcgaacgtct
    11461 gctatgcgag catacctggc gagatcacaa a
//
LOCUS       DATASH010000389         6614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2630592,
            whole genome shotgun sequence.
ACCESSION   DATASH010000389 DATASH010000000
VERSION     DATASH010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6614
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2630592"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VHV99_10885"
     CDS             complement(<1..330)
                     /locus_tag="VHV99_10885"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX3635978.1"
                     /translation="MSTTFERPVRIRFQHCDPAGIVFFPQYLVMTNALVEDWFNEGLQ
                     IDYAQMIAQRRVGLPIVKLDCEFSRPSQMGETITLALSVVALGRRSISIEIVGYCGDE
                     TRFRAKQV"
     gene            complement(327..1517)
                     /locus_tag="VHV99_10890"
     CDS             complement(327..1517)
                     /locus_tag="VHV99_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX3635979.1"
                     /translation="MDLHSALAWPFFEPRHRELAAGIEAWCRATLTHVEHDDVDSTCR
                     HLVRELGEAGWLKYGVGGVAYGGHGDTIDTRAVCLLRETLAKHSGLADFALAMQGLGS
                     GAISLGGTHEQKTRYLPRVANGTSLAAFALSEPEAGSDVAAMALSAREDGDAYVLDGD
                     KTWISNGGIADFYVVFARTGEAPGARGITAFIVDADTPGLEIAERIDVIAPHPLARLH
                     FAGARVPRSQMLGAPGEGFKLAMRTLDIFRTSVAAASLGFARHAMAEGLSRASSRKMF
                     GQTLGDFQLTQAKLAQMALTIDSSALLVYRAAWLRDQGENVTREAAMAKWHASEGAQQ
                     VIDAAVQLYGGMGVQSGTAVEMLYREIRALRIYEGATEVQQLIVGRDLLKAHAAQAIK
                     DAAQ"
     gene            complement(1546..2397)
                     /locus_tag="VHV99_10895"
     CDS             complement(1546..2397)
                     /locus_tag="VHV99_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HEX3635980.1"
                     /translation="MTRSNADALLAGNRLTLAGYEARHFGWSVADNVATITLNRPERK
                     NPLTFESYAELRDLFRQLAYATDVKAVVIHGAGENFCSGGDVHDIIAPLIDLPMPELL
                     LFTRMTGDLVKAMRHCPQPIIAAVDGVCAGAGAILAMSSDMRLGTARSKLAFLFSRVG
                     LAGCDMGACAILPRIIGQGRAAELLFTGRSASGDEGHAWGFYNRLCEPAALLADARKL
                     AADLVAGPTFAHGITKKMLHQEWSMSIDEAIESEAQAQAICMSTRDFERAYSAFAAKS
                     RPVFEGD"
     gene            complement(2432..2989)
                     /locus_tag="VHV99_10900"
     CDS             complement(2432..2989)
                     /locus_tag="VHV99_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX3635981.1"
                     /translation="MSKSSNINKPTAAESKPPRKGVAKPAENVVDLEMSTGADSHMGL
                     RLWLRMLTTTNLVQAELRKRLRNEFDTTLPRFDLMAQLERHPEGLKMTELSRRLMVTG
                     GNVTGITDQLEKEGLVSRDTDPNDRRSISVCLTPAGRKAFDKMAVAHEQWVVELFGGL
                     SLDEKSRTHQRLGKLKQHLLDSLKD"
     gene            complement(2986..3759)
                     /locus_tag="VHV99_10905"
     CDS             complement(2986..3759)
                     /locus_tag="VHV99_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3635982.1"
                     /translation="MNTLSRTLAGQHAVVTGGGSGIGAAVAETLLRAGARVTLMGRNA
                     QRLDAQREKFHALGDVACISVDVTQEDSVANAFAQAGAIDVLINNAGQAQAAPFTHTD
                     MLLWQRMLDVNLTGVFLGTRAVLPGMLERGHGRIVNVASTAGQIGYAYVAAYCAAKHG
                     VIGLTRSLALEVATKGVTVNAVCPGYTETELLHASLEQITSKTARTEQQARETLLRSN
                     PQHRFVSPEQVAHAVLWLCQPGSDAITGQSISISGGEVM"
     gene            complement(3756..6128)
                     /locus_tag="VHV99_10910"
     CDS             complement(3756..6128)
                     /locus_tag="VHV99_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013342286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003959 - NADPH dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="HEX3635983.1"
                     /translation="MRIVCIGGGPAGLYFGLLMKGRNPAYDVTVVERNRPYDTFGWGV
                     VFSDQTLGNLRAADAPSADAILDAFNHWDDIEINFRGRQIRSSGHGFCGIGRKRLLNI
                     LQARCEELGVKLMFETQVTDDTTYEADLIVACDGANSAIRQKHAANYKPAIDMRDCRF
                     VWLGTKKLFDAFTFAFEETEFGWFQAHAYRFDDQTSTFIVETPEHVWRAAGLDEMSKE
                     DSIAFCEKLFARYLDGNALLSNAAHLRGSSQWIRFPRVVNEEWVHWRNNADGSQTPIV
                     LMGDAAHTAHFSIGSGTKLALEDSIELANSIGAHPGDLPAALKHYTDVRSIDVLRIQN
                     AARNSTEWFEHVDRYTSFEPEQFAYSLLTRSQRISHENLRERDARYLSGFEDWLAHRS
                     GIDRAPEKHSVPPMFTPFTLRGVTLKNRVVVSPMAQYSAVDGIAGDYHLMHLGARAMG
                     GAALVMTEMTCVSPEARITPACPGMYAPEHLTAWKRIVDLVHGQSDAKIGIQLGHSGA
                     KGSTRVAWEGIDQPLDEGNWPLVSASPQQYLSGISQHSREATKDELNEIEAQFVRATQ
                     MSAQAGFDWLELHCAHGYFLSSFLSPLTNHRTDEYGGSLENRLRYPLQIFKAIRAVWP
                     QDKPISVRISANDWVEGGTTPDDAVQIARAFKTAGADMIDVSSGQVSKQEKPVFGRMF
                     QTPFADRVRNEAGIATIAVGAISEADHVNSIIAAGRADLCAIARPHLANPSWTLNEAA
                     KIGYFDVMWPKPYTAAKSQLERNLERERAQVAANAGLSPQERAQRAEGTT"
     gene            6368..>6614
                     /locus_tag="VHV99_10915"
     CDS             6368..>6614
                     /locus_tag="VHV99_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012365284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3635984.1"
                     /translation="MFHLKTTLVHAKTACALVLMAMLTQVGAQANAVTVNHAADVETS
                     ASGKSLVAGPFVADDPVAIRDTLANRNINAAPPSLSDR"
BASE COUNT         1173 a         1999 c         2110 g         1332 t
ORIGIN      
        1 cacctgtttt gcgcgaaagc gggtttcgtc gccgcaatag ccgacgatct cgatgctgat
       61 cgagcggcgg ccgagcgcca cgacgctgag tgccagcgtg atcgtttcac ccatctggct
      121 cggccgcgaa aactcgcaat caagtttgac gatcggcaag ccgacgcggc gttgcgcgat
      181 catctgcgca taatcgatct gcaggccttc gttgaaccag tcttcgacca acgcgttggt
      241 catcaccaga tattgcggaa agaacacgat gccggccgga tcgcagtgct ggaagcggat
      301 gcggaccggt ctttcgaagg tcgtgctcat tgcgctgcgt ccttgatggc ttgcgcggcg
      361 tgggccttca aaaggtcgcg gccgacgatc aattgctgca cttcagtggc gccttcgtaa
      421 atccgcaagg cacgaatctc tcgatacagc atctcgacgg cggtgccgct ttgcacgccc
      481 atgccgccat aaagttgcac ggcggcgtcg atcacctgtt gcgcgccttc gctcgcgtgc
      541 catttagcca tcgcggcttc acgcgtgacg ttttcgcctt gatcgcgcaa ccaggccgcg
      601 cgataaacca gcagtgcgct gctgtcgatg gtcaatgcca tctgcgccag cttggcttgc
      661 gtcagctgaa aatcgccgag cgtctgtccg aacatcttgc gcgacgaggc gcgcgagagg
      721 ccttctgcca tcgcatgccg cgcgaagccc agcgacgcgg ccgccaccga ggtgcggaag
      781 atatcgagcg tacgcatcgc aagtttgaaa ccttcgccgg gtgcgccgag catctggctg
      841 cgcggcacgc gtgcaccggc aaaatgcaag cgagcaagcg gatgcggcgc gatgacgtcg
      901 atgcgttcgg caatttccag gcccggcgtg tcggcatcga cgatgaaggc ggtgatgccg
      961 cgcgcgcctg gcgcttcgcc ggttctggcg aacaccacat agaaatcagc gatgccaccg
     1021 ttcgagatcc atgtcttgtc gccgtcgagc acgtaggcgt cgccgtcttc acgcgcgctt
     1081 agtgccatcg cggcgacgtc cgatcccgct tccggttccg acaaagcgaa cgcggcaaga
     1141 gaggtgccgt tcgccacgcg cggcaaatag cgcgtttttt gctcgtgcgt gccgccgagc
     1201 gagatcgcgc ctgagccgag cccttgcatt gccaaggcga aatccgcgag gccggaatgt
     1261 ttggcgagcg tctcgcgcag caggcacacg gcgcgcgtgt cgatggtgtc gccgtggcca
     1321 ccataggcca cgccgccgac gccgtatttc agccagcctg cctcgccgag ttcacggacc
     1381 agatgacggc aggttgaatc gacatcgtcg tgctcgacat gcgtgagagt cgcgcggcac
     1441 catgcttcga tacccgcggc cagttcccgg tggcgcggtt cgaagaacgg ccaggccagt
     1501 gcgctgtgca gatccatatc gcccttatcg ctttgagtgc tcaattcaat ccccttcgaa
     1561 cacagggcgc gacttcgcgg caaacgcgct ataggcccgt tcgaaatcgc gtgtgctcat
     1621 gcagatggcc tgggcttgcg cttccgattc gatcgcttcg tcgatgctca tgctccattc
     1681 ctggtgcagc atcttcttcg tgatgccgtg cgcgaaggtc gggccggcga cgagatcggc
     1741 ggcgagcttc cgggcgtccg cgaggagcgc cgccggttcg cacaggcggt tatagaagcc
     1801 ccatgcgtgg ccttcgtcgc cactcgccga gcggccggtg aacagcaatt cggcggcgcg
     1861 tccctggccg atgatgcgcg gcaggatcgc gcatgcgccc atatcgcaac ccgcgaggcc
     1921 gacgcgtgag aacaggaacg cgagcttgct gcgcgcggtg cccaggcgca tatcggacga
     1981 catcgccaga atcgcaccgg cgccggcgca gacgccgtca accgcggcaa tgatgggctg
     2041 cgggcaatgg cgcatcgctt tgacgaggtc gcctgtcatg cgcgtgaaca gcagcagttc
     2101 cggcatcggc agatcgatca gcggcgcgat gatgtcgtgc acatcgccgc cggagcagaa
     2161 gttttctccc gcgccgtgaa tgacgactgc cttgacgtcg gtggcgtagg ccagttgccg
     2221 gaacagatcg cgcaattcgg catacgactc gaacgtcagc gggttcttgc gctcggggcg
     2281 gttcagcgtg atcgtcgcga cattgtcggc gaccgaccag ccgaagtgcc gcgcctcgta
     2341 accggcgagc gtcaggcggt tgccggccag cagggcgtcg gcgttggatc gtgtcatgtg
     2401 tgtctccatg caagctcgtt ttcggtgcgg gtcaatcttt caaactgtcg agcagatgtt
     2461 gtttgagttt gccaagacgc tgatgagtac gcgatttttc gtcgaggctc aagccgccga
     2521 acaactcgac gacccattgc tcatgcgcca ccgccatctt gtcgaacgcc ttgcggcctg
     2581 ccggcgtcag gcacacgctg atcgagcggc gatcgttcgg atcggtatcg cgcgacacca
     2641 ggccttcttt ttcgagctga tcggtaatgc cggtgacatt gccgcccgtc accatcaaac
     2701 ggcgcgacaa ctcggtcatc ttcaggcctt ccggatgacg ttcgagctgc gccatcaaat
     2761 cgaatcgcgg cagcgtagtg tcgaattcgt tgcgcaagcg tttgcgcagt tcggcttgca
     2821 cgagattggt cgtggtcagc atgcgcagcc acaaacgcaa gcccatgtga ctatccgcgc
     2881 cagtgctcat ttccagatcc acgacgttct ccgctggttt ggcgacgcct ttgcgcggtg
     2941 gtttgctttc cgccgccgtg ggcttgttga tgttcgatga cttgctcaca ttacttctcc
     3001 accggagatg gaaatggatt ggcccgtgat tgcgtccgag ccgggttggc agagccatag
     3061 cacggcatgg gcgacctgct ccggactcac gaagcgatgt tgcggattcg aacgcagcaa
     3121 ggtttcgcgc gcctgctgtt cggtgcgcgc ggtcttgctg gtgatctgtt cgagcgatgc
     3181 gtgcagcagt tcggtttccg tataaccggg acagactgcg ttgacggtga cgcctttcgt
     3241 cgcgacttcc agcgcgagtg aacgggtcaa accgattacg ccatgtttcg cggcgcagta
     3301 agcggcgacg taggcgtagc cgatttgccc cgccgtgctc gcgacgttga cgatgcggcc
     3361 gtggccgcgt tcgagcatgc cgggcaacac ggcgcgcgta ccgaggaaca caccggtgag
     3421 gtttacgtcg agcatgcgct gccagagcag catgtcggta tgcgtgaacg gcgcggcttg
     3481 cgcctggccc gcgttgttga tgaggacgtc gattgcgccg gcttgcgcga atgcattggc
     3541 tacggagtct tcctgcgtga catcgacact gatgcaagca acatcgccca gcgcgtggaa
     3601 tttttcccgc tgcgcatcga ggcgttgcgc gttacggccc atcaaggtga cgcgcgcgcc
     3661 ggcacgcagc agcgtttcgg ccaccgccgc gccaatgccg ctgccgccgc ctgtgacgac
     3721 ggcatgttgt ccggcgagcg ttctgctgag cgtattcacg tggttccctc cgcgcgttgc
     3781 gcgcgttctt gtggcgacag ccccgcattc gcggcgacct gggcacgctc acgttcgaga
     3841 ttgcgttcga gttgcgactt cgcggcggtg taaggcttcg gccacatcac gtcgaaatag
     3901 ccgatttttg cggcctcatt gagtgtccac gacggattcg ccagatgcgg ccgcgcaatc
     3961 gcgcagagat cggcgcggcc cgccgcgatg atgctgttca cgtggtcggc ttcagagatc
     4021 gcgccaacgg cgatcgtcgc aatgccggcc tcgttgcgca cgcggtcggc gaatggcgtc
     4081 tggaacatgc ggccaaagac gggcttttcc tgcttgctga cctggcccga cgatacgtcg
     4141 atcatgtcgg cgcccgccgt tttgaaggca cgggcgattt gcacggcgtc gtcgggcgtc
     4201 gtgccgcctt cgacccaatc attcgcggaa atccgcacgg aaatcggctt gtcttgcggc
     4261 cagacggcgc ggatcgcctt gaagatctgc aacgggtagc gcaggcggtt ttcgagcgag
     4321 ccgccgtatt cgtccgtacg atggttggtc aacggcgaca ggaagctcga gaggaaatag
     4381 ccgtgtgcgc aatgcagttc gagccaatcg aagcctgcct gggcggacat ttgcgtggcg
     4441 cgtacgaact gcgcttcgat ttcgttcaac tcgtctttgg tggcttcgcg cgagtgctgg
     4501 ctgatgccgc tcaaatactg ttgcggtgaa gcggagacga gcggccagtt gccttcgtca
     4561 agcggctggt cgatgccttc ccatgccacg cgcgtcgagc cttttgcgcc ggagtggccc
     4621 agctgaatgc cgatcttcgc gtcggactgt ccatgcacga ggtcaacaat gcgcttccat
     4681 gccgtgagat gttccggcgc atacatgccg ggacaagcgg gcgtgatgcg cgcttcaggc
     4741 gacacgcagg tcatttccgt catcaccagc gcggcgccgc ccattgcacg tgcgccgaga
     4801 tgcatcaggt gatagtcgcc ggcgataccg tcgacggctg agtactgtgc catcggcgag
     4861 acgaccacgc gatttttcag cgtgacgccg cgcaacgtga agggcgtgaa catcggcggg
     4921 acggagtgct tttcaggcgc gcggtcaatg cctgagcgat gtgcgagcca gtcttcgaag
     4981 ccggacaggt agcgcgcatc gcgctcgcga agattctcgt gcgaaatgcg ctgcgagcgc
     5041 gtaagcagcg aatacgcgaa ctgttcaggc tcgaatgacg tatagcggtc gacatgctcg
     5101 aaccactccg tcgagttgcg cgcggcgttc tgaatgcgca gtacgtcgat gctgcgcacg
     5161 tccgtgtaat gtttcagtgc ggccggcaaa tcgcccggat gcgcgccgat actgttggcc
     5221 agttcgatcg aatcttcaag cgcgagcttg gtgcccgagc cgatcgagaa atgcgcggtg
     5281 tgcgccgcgt cgcccatcag cacgatcggt gtctggctgc cgtcggcgtt attgcgccaa
     5341 tgtacccatt cctcgttgac gacgcgcggg aagcgaatcc actgcgacga accacgcaaa
     5401 tgcgcggcgt tcgatagcag cgcattgccg tccagatacc tggcgaacag cttctcgcag
     5461 aacgcgatgc tgtcttcctt gctcatctcg tcgagaccgg cggcgcgcca cacatgttcc
     5521 ggtgtttcga caatgaaggt ggaggtctgg tcgtcgaaac gataggcgtg cgcctggaac
     5581 cagccgaatt cggtttcttc gaacgcgaag gtgaaggcgt cgaaaagttt tttggtgccg
     5641 agccagacga agcggcagtc gcgcatgtcg atggcgggct tgtagttggc cgcgtgtttc
     5701 tggcggatcg cgctgttggc gccgtcgcag gcgacgatca ggtcggcttc gtaggtcgtg
     5761 tcgtccgtga cctgggtttc gaacatcagc ttgacgccca gctcttcgca gcgcgcctgc
     5821 aggatattca gcagacgttt gcggccaatg ccgcagaagc catggcccga cgagcggatc
     5881 tgcctgccgc ggaaattgat ctcgatgtcg tcccagtggt tgaaggcgtc gaggatcgcg
     5941 tcggcgctcg gagcgtcggc ggcgcgcagg ttgccgagcg tctggtcgga gaatacgacg
     6001 ccccagccga aggtgtcgta agggcggttg cgttcgacca ccgtgacgtc ataggccggg
     6061 ttccggccct tcattaacag gccgaagtac aacccggccg ggccgccgcc gatacagacg
     6121 atgcgcatgc tgactccccc acgtggactt tgctctggag ggtaatttag gtctatatag
     6181 tttagatgtc aagtaaatgg tggggcgggg gcagcacgag tattttgcag gtcacgtagg
     6241 aaacgataca gtgagggcgt ccgcgatcga agtgccaacg ttgcgcgaat cgtgaatctc
     6301 gatctgccac gccaaccgta taaccatggc aatcagcagg ccccgccgga ataccaggag
     6361 ttttgtcgtg tttcatctga aaaccacgct ggttcatgcg aagaccgcgt gtgccctcgt
     6421 tctcatggcg atgctgacgc aagtcggcgc acaagcgaac gcagtcactg tcaaccatgc
     6481 ggcagacgtc gagacctctg cttctggcaa atctttggtg gcaggtccat ttgtcgccga
     6541 cgaccccgtt gccattcggg acacgttggc gaaccggaac attaatgccg ccccgccttc
     6601 cttaagcgat cgcg
//
LOCUS       DATASH010000390         1621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1642893,
            whole genome shotgun sequence.
ACCESSION   DATASH010000390 DATASH010000000
VERSION     DATASH010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1642893"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="VHV99_10920"
     CDS             <1..427
                     /locus_tag="VHV99_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEX3635985.1"
                     /translation="VDSSVERLEVRALGLNGNRHVLTVNGVPVPLQPTGRVSEYVAGV
                     RFRAWSQPSALHPTIGVDAPLTFDLVDTWTGRSVGGCQYHVAHPGGRNYKTFPVNAYE
                     AESRRRARFFASGHTPGPLTVAMPQRSLEFPFTLDLRYW"
     gene            475..>1621
                     /locus_tag="VHV99_10925"
     CDS             475..>1621
                     /locus_tag="VHV99_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEX3635986.1"
                     /translation="MAFQSTFPFETSAARADASSLLRLLPAYEGHWDELRDASGALRE
                     PWRQFFERLGEDGIARLEDHYASIAQQIRDNDISYNVYADNGEPRPWALDLLPFLISE
                     AEWAHIERGVTQRAHLLNAIVADIYGSQTLLERGQLPPALVFGHPGYLRSVKGFKPPG
                     GQYLQVVAVDLARTPAGSWTVMAHRTEAPSGLGYALENRLIVSTLFAEPFRALHVSRL
                     APTYSQLIATLAQAAQATMQHDGTGTDTSPHIALLTPGPFSETYFEHVFLARYLGVTL
                     VEGKDLTVRDDMLYLKTLAGLERVHVVLRRLDDAFCDPVELRADSTIGVPGLLQVMRA
                     GNVIVSNVPGSGFVESPALHGFLPGICHDLLNEELVLPGVPTWWCGEE"
BASE COUNT          265 a          507 c          528 g          321 t
ORIGIN      
        1 tgtggattcg tcggtggagc ggctcgaagt gcgcgcgctc ggtttgaacg gcaaccgtca
       61 tgtgctgacc gtcaacggcg tgccggtacc gttgcaaccg accggccgcg tcagcgaata
      121 tgtggccggc gtgcgctttc gtgcgtggtc gcaaccgtcg gcgttgcatc cgaccatcgg
      181 cgtggatgcg ccgctcactt tcgatctggt cgatacatgg acaggccgct cggtcggcgg
      241 atgccagtat catgtcgctc atccgggcgg gcgcaactac aagaccttcc cggtcaacgc
      301 atacgaggcg gaaagccggc ggcgcgcgcg cttcttcgcg tccggtcata cgccggggcc
      361 gctcacggtc gcgatgccgc agcgcagcct cgagtttccg ttcacgctcg acctgcggta
      421 ctggtaacct ggtagactga aacgcggcaa cacactttta aaaacgacac gatcttggcc
      481 tttcaatcga cttttccctt cgagacgtcc gcagcgcgcg cggacgcttc gtccttattg
      541 cggctgttgc cggcctatga aggacactgg gacgagttac gcgacgcctc aggcgcattg
      601 cgcgaaccgt ggcggcaatt cttcgagcgg cttggcgaag acggcatcgc gcgactggaa
      661 gaccactacg cgtcgatcgc gcagcagatc cgcgacaacg acatcagcta caacgtctac
      721 gcggataacg gcgagccgcg gccgtgggcg ctcgacctgc tgccgttcct gatcagcgag
      781 gccgagtggg cgcatatcga gcgcggcgtg acgcagcgcg cgcatctgct caatgcaatc
      841 gtcgccgata tctatgggtc gcagaccttg ctcgaacgtg ggcaattgcc gcccgcgctg
      901 gtattcgggc atcccggcta tctgcgttcc gtgaagggct ttaagccgcc tggcgggcaa
      961 tatctgcagg tggtcgccgt cgatctggcg cgtacgcccg ccggtagctg gaccgtcatg
     1021 gcgcatcgca ccgaggcgcc gtccggcctc ggttatgcgc tggaaaaccg tctgatcgtc
     1081 tcgacgctgt tcgccgaacc gttccgcgcg ctgcatgtga gccgcctcgc gccgacctat
     1141 tcgcaactga tcgccacgct cgcgcaggcc gcgcaagcca cgatgcagca cgacggtacg
     1201 ggcaccgata cttcgccgca tatcgcgttg ctcacgccgg ggccatttag cgaaacctat
     1261 ttcgagcacg tctttctcgc gcgctatctc ggcgtcacgc tagtggaggg caaggacctg
     1321 accgtgcgcg acgacatgct gtatctgaag acgctcgctg gtctggagcg ggtgcatgtg
     1381 gtgctgcggc ggctggatga cgcgttctgc gatccggtcg aactgcgcgc ggattcgacc
     1441 atcggcgtac ccggtctgtt gcaggtgatg cgggcgggca atgtgatcgt gtcgaacgtg
     1501 ccgggttccg gcttcgtcga atcgcccgcc ttgcatggct ttctgccggg catttgtcat
     1561 gatttgctga acgaggaact ggtgctgccg ggcgtgccca cgtggtggtg cggcgaagaa
     1621 g
//
LOCUS       DATASH010000391        14218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_58491,
            whole genome shotgun sequence.
ACCESSION   DATASH010000391 DATASH010000000
VERSION     DATASH010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 14218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14218
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58491"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..570)
                     /gene="rlmH"
                     /locus_tag="VHV99_10930"
     CDS             complement(100..570)
                     /gene="rlmH"
                     /locus_tag="VHV99_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEX3635987.1"
                     /translation="MKLHILAVGHKMPDWIATGFDEYAKRMPPELRIELREIKPEQRS
                     SGRPAESVMAAERQKIETALPKNARIVALDERGKDWTTMQLAGALPGWQQDGRDVAFL
                     IGGADGLDPDLKARADMLLRVSSLTLPHAMVRVLLAEQLYRAWTITQNHPYHRV"
     gene            complement(728..1183)
                     /gene="rsfS"
                     /locus_tag="VHV99_10935"
     CDS             complement(728..1183)
                     /gene="rsfS"
                     /locus_tag="VHV99_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEX3635988.1"
                     /translation="MDIRKLQRVIVDALEDVKAQDIKVFNTSHLTALFDRVIVASGTS
                     NRQTKALASSVRESVKENGGDIISTEGEDIGEWVLVDCGDAIVHILQPALRQYYNLEE
                     IWGDKPVRIKLSTPNPFGGARSSEPDDEDEEEDVPAVKKPARKAPARRK"
     gene            complement(1188..1937)
                     /locus_tag="VHV99_10940"
     CDS             complement(1188..1937)
                     /locus_tag="VHV99_10940"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEX3635989.1"
                     /translation="MHVTTTKDLHLKPNAHPVALPRRIGLLGGTFDPIHDGHLALARR
                     FADVLNLTELVLLPAGQPWQKTDVSAAVHRLAMTRAAAGALVLPGVTVRVATDEIEHD
                     GPTYTVNTLQLWREREGPDASIALLIGADQLVHLDSWRDWRRLFDFAHICAATRPGFD
                     LASIPPVVAKEIDARRARADVLQATPCGHLLIDTTLAFNVSATDIRAHLREQVNQRLA
                     LVGEEQQDQAAASHVPTAVWDYILQHHLYHR"
     gene            complement(1966..2889)
                     /gene="hemF"
                     /locus_tag="VHV99_10945"
     CDS             complement(1966..2889)
                     /gene="hemF"
                     /locus_tag="VHV99_10945"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006405601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HEX3635990.1"
                     /translation="MNDSTYDAQAVRSWLQGLQTHIADTLGAFDGKPFATDAWQRGPG
                     EKLRGGGCTRILEGGNVFERAGIGFSDVAGDTLPGSASAARPQLAGRGFEAMGVSLVL
                     HPHNPHCPTVHMNVRLLIATKAGEEPVFWFGGGMDLTPYYGYEEDAQHFHGVCRDALQ
                     PYGADLYPSFKRWCDEYFFLKHRDEPRGIGGIFFDDFSALGFDQSFAMLKSVGEGFLK
                     AYLPIIEKRRNIPYGEAERDFQAYRRGRSVEFNLVFDRGTLFGLQSGGRTESILMSMP
                     PVVNWRYNWQPEPGTPEARLYSDFLVPRDWV"
     gene            complement(3125..4423)
                     /gene="purD"
                     /locus_tag="VHV99_10950"
     CDS             complement(3125..4423)
                     /gene="purD"
                     /locus_tag="VHV99_10950"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEX3635991.1"
                     /translation="MKLLVVGSGGREHALAWKLAQSPRVQLVYVAPGNGGTAQDERLR
                     NIDITDPAALADFVEKEQVAFTLVGPEGPLAAGIVNLFRSRGLKIFGPSKEAAQLESS
                     KDFAKAFMKRHAIPTAEYETFADVAAAHAYLDAKGAPIVIKADGLAAGKGVVVAQTLE
                     EAHAAVDMMLSDNKLGDAGARVVIEEFLAGEEASFIVMVDGKHVLALASSQDHKRLLD
                     ADQGPNTGGMGAYSPAPIVTPQLHARVMREIIQPTVRGMEKEGIRFTGFLYAGLMIDA
                     QGNPKTLEFNCRMGDPETQPIMARLKGDFSKVVEQAIAGTLNTIELEWDRRTALGVVL
                     AAHNYPDTPRKGDRISEIPAETDDSVTFHAGTTLADGKLTTSGGRVLCVVGLADSVRS
                     AQSAAYETINQISFDGMQYRRDIGYRALNRKHDAKTEGKH"
     gene            complement(4672..5400)
                     /locus_tag="VHV99_10955"
     CDS             complement(4672..5400)
                     /locus_tag="VHV99_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006763795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX3635992.1"
                     /translation="MAGHSKWANIKHKKAAADAKRGKVWTRLIKEIQVAARMGGGDID
                     SNPRLRLAVEKAYDANMPKDNVNRAIQRGVGGVDGANYEEIRYEGYGIGGAAVIVDTM
                     TDNRTRTVADVRHAFSKNGGNMGTDGSVSFMFDHVGQFLFAPGTPEDKLMEAALEAGA
                     DDVVTNEDGSIEVLCPPNDFPKVKTALEAAGFKAELAEVTMKPQTEVEFTGDEAVKMQ
                     KLLDALESLDDVQEVYTNAAIADE"
     gene            5868..6062
                     /locus_tag="VHV99_10960"
     CDS             5868..6062
                     /locus_tag="VHV99_10960"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEX3635993.1"
                     /translation="MNTGGPVKQSEFRRWLADQGATFEQGSKHLKVYLNGRQTTLPRH
                     PAHEISEPLRKAILKQLKCK"
     gene            6132..6554
                     /locus_tag="VHV99_10965"
     CDS             6132..6554
                     /locus_tag="VHV99_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008162700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX3635994.1"
                     /translation="MFRYPARIEPDEAGFTVSFRDIPEALTSGATLEEAREMAADALA
                     TAMDFYFEDKRQVPLPSKARRGEELIELPASLAAKVLLLNEMLAQRVTAAELARRLHT
                     RAQDIQRVIDLGHVTKIDTIASALAALGKRLELSIVPA"
     gene            complement(6637..7287)
                     /gene="upp"
                     /locus_tag="VHV99_10970"
     CDS             complement(6637..7287)
                     /gene="upp"
                     /locus_tag="VHV99_10970"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010091140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEX3635995.1"
                     /translation="MTQDSRFPNLFILDHPLIQHKLSHMRDRDTSTRTFRELLREITL
                     LMGYEITRNLPMTTRRLTTPLVEIDAPVIAGKKLAIVPVLRAGIGMSDGLLELVPSAR
                     VGHIGVYRAEDHRPVEYLVRLPDLEDRVFILCDPMVATGYSAVHAVDVLKRRNVAGEN
                     IMFLALVAAPEGVQVFQDAHPDVKLYVASLDSHLNEHAYIVPGLGDAGDRLFGTKN"
     gene            7667..8449
                     /locus_tag="VHV99_10975"
     CDS             7667..8449
                     /locus_tag="VHV99_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3635996.1"
                     /translation="MDMGIAGRTALVCAASKGLGRGCAEALAAEGVNLTIVARTAETL
                     EATAAEIRKQSGVEVKTVACDITTPEGRAAALAACPQPDILVNNAGGPPPGDFRNFTH
                     EDWIRALESNMLTPIELIRATIDGMSERGFGRIVNITSSAVKAPIDVLGLSNGARSGL
                     TGFIAGLARSKVAGSGVTINSLLPGIFDTDRIATTFAAQAKAQNISVDDARKQRMKTI
                     PAGRFGNRDEFGRACAFLCSVHAGYITGQNWLIDGGTYPGTF"
     gene            8526..8915
                     /locus_tag="VHV99_10980"
     CDS             8526..8915
                     /locus_tag="VHV99_10980"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HEX3635997.1"
                     /translation="MSTRIALIAHDHKKDDIVKLAGEYVDTLRRCEIVATGTTGTRIG
                     DAHGLTVERMLSGPHGGDLQIGAQLAEGRVDMVIFLRDPMTPQPHEPDINALVRACDV
                     HNIPCATNISTARMILDVLTLRLTQQV"
     gene            8965..9939
                     /locus_tag="VHV99_10985"
     CDS             8965..9939
                     /locus_tag="VHV99_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006405597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEX3635998.1"
                     /translation="MAKAIRFDKTGGPEVMKWVDVEVGEPGAGEIRIKQNAVGLNYID
                     VYFRTGLYPLPLPGGLGMEAAGEVTAVGTGVSGFKAGDRVAYVARPPGAYAQERVLSA
                     AQVVKVPDALSDEQAASVMLQGLTAQYLLRRTYPVKAGDTILIQAAAGGVGLLVCQWA
                     KALGATVIGTVGSDEKAEIAKAHGCDHAIVYTREDFTRRVREITNGAGVPVVYDSIGK
                     DTFTGSLDCLAPLGMFVSFGNASGPLPPIDSSEFAGRGSLFFTRPTLFTYIAKRSDYE
                     AMSAELFDVLVSGKVKTSINQRHALADVGRAHADLEGRRTTGSTVLLP"
     gene            10198..10311
                     /locus_tag="VHV99_10990"
     CDS             10198..10311
                     /locus_tag="VHV99_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007583456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium ABC transporter ATPase"
                     /protein_id="HEX3635999.1"
                     /translation="MDVLYVGGLVLFSALTFALIAGCEKLMQFRRGQGVRS"
     gene            10308..10400
                     /gene="kdpF"
                     /locus_tag="VHV99_10995"
     CDS             10308..10400
                     /gene="kdpF"
                     /locus_tag="VHV99_10995"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017331558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HEX3636000.1"
                     /translation="MNWILWLSGAATALLFAYLVYALLRAEDIE"
     gene            10397..12202
                     /gene="kdpA"
                     /locus_tag="VHV99_11000"
     CDS             10397..12202
                     /gene="kdpA"
                     /locus_tag="VHV99_11000"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEX3636001.1"
                     /translation="MNSNNVLQAGLFIVVLLLAAVPMSRYMTNVMDGTSRVVRFGGPL
                     ERLLYRIAGVDPSTEMSWKHYAIATIAFNVLGVLFLYVLLRVQGWLPGNPQQFGPMTV
                     DGAFNTAVSFVTNTNWQDYTPEQTVGYLTQMLGLTVQNFFSAATGIVVVIALIRGFAR
                     HTAQTIGNFWVDLTRVTLYVLLPTSVIVAAVLMSQGVIQNFKAYEDVPTLQTTTYAAP
                     KLDAQGNPVKDAKGNAVTVDTPVKTQTLAMGPVASQEAIKMLGTNGGGFFNGNSAHPY
                     ENPTPFSNFLQIFSMLIIPASLALVFGRMIMDRKQGVAVLATMAIAFGVCVFGEIGAE
                     QSGNPALTALHVDQSATALQSGGNAEGKETRFGIAQSGIFTVATTAASCGAVANTHDS
                     LTPIGGLYPMLLIQLGEVIFGGVGSGMYGMLVFAMLAVFVAGLMIGRTPEYVGKKIEA
                     YEMKMVSIVVLLTPLLVLVGTSIAVLTDAGKAGIANPGAHGFSEILYAFSSAANNNGS
                     AFAGLTVSTPFYNWLTAIAMWFGRFGTVIPVLAIAGSLAAKKRIGVTGGTLPTHGPLF
                     VVLLLGTVLLVGALTYVPALALGPGVEHLIMMGAH"
     gene            12279..>14218
                     /gene="kdpB"
                     /locus_tag="VHV99_11005"
     CDS             12279..>14218
                     /gene="kdpB"
                     /locus_tag="VHV99_11005"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011352735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HEX3636002.1"
                     /translation="MTEHNATRSMFDPALLRPAIVDSFKKLTPRTQFRNPVMFCVYVG
                     SILTTILWIAALGGQAEAPAGFILAVTLWLWFTVLFANFAEALAEGRSKAQAASLRSA
                     KKDVMAKKLNEPHPKSPIRILTASDLRKGDVVLVETGDVIPADGEVIDGVASVDESAI
                     TGESAPVIRESGGDFSSVTGGTRVLSDWIVVRVTANPGEAFLDRMIAMVEGAKRQKTP
                     NEIALTILLVALTIVMLLATATLLPFSMFSVEAAKAGHVVTITALVALLVCLIPTTIG
                     GLLSAIGVAGMSRMMQANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASQFVPAP
                     GLTEEALADAAQLSSLADETPEGRSIVVLAKQRFNIRQRDMASLHAVFLAFTAQTRMS
                     GVDLPGREIRKGASDAVKHYVEANGGRFPNEVSAAVAEVARRGSTPLVVAEKGEPGAR
                     VLGVIELKDVVNGGIKERFAELRKMGIKTIMVTGDNRLTAAAIAAEAGVDDFLAEATP
                     EAKLATIRAHQAEGRLVAMTGDGTNDAPALAQADVAVAMNTGTQAAKEAGNMVDLDSN
                     PTKLIEIVEIGKQMLMTRGSLTTFSIANDVAKYFAIIPAAFATTYPALNALNVMHLAT
                     PASAIMSAVIFNALIIVLLIPLA"
BASE COUNT         2531 a         4397 c         4652 g         2638 t
ORIGIN      
        1 atgagcaggc cgaaaaacgc gtcgaagaac ttgcctataa gcccgtcaat ccgcgcaggc
       61 gacgagaccg gaaagcgcgc cgtcagcagg ctcgttcgct cacacgcggt gataggggtg
      121 attttgcgtg atggtccacg cgcggtaaag ctgttcggcg agcagcacgc gcaccatcgc
      181 gtgcggcagc gtcaggctgg agacgcgcag cagcatatcg gcgcgcgctt tcagatcggg
      241 atcgagcccg tcggcgccgc cgatcagaaa cgccacgtcg cggccgtcct gctgccagcc
      301 gggcagggcg ccggcgagct gcatcgtggt ccagtccttg ccgcgttcat cgagcgcgac
      361 gatgcgcgcg ttcttcggca acgcggtttc gattttctgc cgctcggcag ccatcacgct
      421 ttcggctgga cgccccgacg aacgctgctc gggcttgatc tcgcgcagct cgatacgcag
      481 ctcgggtggc atgcgcttcg cgtattcgtc gaagccggtg gcgatccagt ccggcatctt
      541 gtggccgaca gcgaggatat gcagtttcat ggcggatgta atcgcggatg caatcacggg
      601 tgaaatagag ggcgcaagcg ctgcaatcga gggcgcaaag ggctgcaatt acggacgcaa
      661 gcgctgcaat cgcggacgca agcgccgact acgcgtcgcg gacttcagcg ttttgccagc
      721 ccggcgctta cttgcggcgt gccggcgcct tgcgtgcggg ctttttgaca gccggcacgt
      781 cttcttcttc gtcctcgtcg tccggctcgc tagagcgcgc gccgccgaac ggattcggcg
      841 tcgacagctt gatgcgcacc ggcttgtcac cccagatttc ctcaaggttg tagtactggc
      901 gcagcgccgg ttgcaggatg tgcacgatcg cgtcgccgca atcgaccagc acccattcgc
      961 cgatgtcctc gccctcggtg ctgatgatgt cgccgccgtt ttccttgacg ctctcgcgca
     1021 cgctcgaagc gagcgccttg gtctggcggt tcgaggtgcc gctcgcgacg atcacgcgat
     1081 cgaacaacgc cgtcaggtgg ctggtgttga acaccttgat gtcttgcgct ttgacgtctt
     1141 cgagagcgtc gacgatcacg cgttgcagtt tgcgaatatc catggggtta ccggtggtac
     1201 agatgatgtt gaagaatata gtcccacacc gcggtgggga catggctcgc ggcggcctgg
     1261 tcttgctgtt cctcgcccac gagggcgagc cgctggttca cctgttcgcg cagatgcgcg
     1321 cgaatgtcgg tggccgagac gttgaacgca agcgtcgtat cgatcagcaa gtggccgcag
     1381 ggggtggctt gcagaacgtc ggcgcgggca cggcgcgcgt cgatttcttt agcgaccacg
     1441 ggcggaatcg acgcgagatc gaaaccgggg cgcgtggccg cgcagatgtg agcaaagtcg
     1501 aacaggcgcc gccagtcgcg ccagctgtcg agatgcacca gttgatccgc gccgatcaga
     1561 agcgcaatcg acgcatccgg accttcacgt tcacgccaca attgaagcgt gttgacggtg
     1621 taggtcggac cgtcgtgttc aatttcgtcg gtcgccaccc gcacggtcac gcccggcagc
     1681 accaacgcgc cggcggcggc gcgggtcatg gccagcctgt gaacagccgc tgacacgtcg
     1741 gttttctgcc agggctggcc ggccggcagc agcaccagtt cggtcaggtt cagcacgtcg
     1801 gcgaaacgcc gcgcgagcgc gagatggccg tcgtggatcg gatcgaaagt gccgccgagc
     1861 agtccgatcc ggcgaggcaa ggcgacagga tgagcgttcg gcttcaggtg aagatccttt
     1921 gtcgtggtca cgtgcagtgt ggtgcgggcc tgacgggcct aagcctcaca cccaatcgcg
     1981 cggcacgaga aaatcgctgt aaagacgcgc ttccggcgtg cccggttccg gctgccagtt
     2041 gtagcgccag ttcaccacag gcggcatgga catcagaatc gattcggtgc gtccgccgct
     2101 ctgcagcccg aacagtgtgc cacggtcgaa gaccagattg aactcgacgg aacggccgcg
     2161 ccggtatgcc tggaagtccc gctcggcttc gccgtacgga atgttgcggc gcttttcgat
     2221 gatcggcagg tacgctttga ggaatccctc gccgacgctt ttcagcatcg cgaacgactg
     2281 atcgaagccc agcgccgaga agtcgtcgaa gaaaatcccg ccaatgccac gcggttcgtc
     2341 gcggtgcttg aggaagaaat actcgtcgca ccagcgcttg aaactcgggt agaggtccgc
     2401 gccataaggc tgcagcgcgt cgcggcagac accatggaaa tgctgcgcgt cttcctcgta
     2461 gccgtagtac ggcgtcagat ccatgccgcc gccgaaccag aataccggct cttcgccggc
     2521 tttggttgca atcagcagac gcacgttcat atgcacggtc gggcagtgcg gattgtgcgg
     2581 gtgcagcacg agcgagacgc ccatggcttc gaaaccacgg ccggccagtt gcgggcgcgc
     2641 ggcgctcgcc gaaccgggca gcgtgtcgcc cgctacgtcc gagaagccga tgccggcgcg
     2701 ttcgaacacg ttgccgcctt ccagaatccg cgtgcagccg ccgccgcgca gcttttcgcc
     2761 ggggccgcgc tgccacgcgt cggtcgcgaa cggcttaccg tcgaacgcgc cgagcgtgtc
     2821 cgcgatatgc gtttgcaggc cttgcagcca gctgcgcacg gcctgtgcgt cataggtcga
     2881 atcgttcatg aatgcagatg ctgagggcgg cacgctcgac gtgccgcggg ttcttcgtga
     2941 agggtgacgg ccgggccttc gtcaggatgt tcctgctcct gcgaaggcgc ggcggcacat
     3001 ttgttcgcgc cgggtgaatg ccgctcgacc cggggcgccc acatggtgcg gtgggcgcgg
     3061 ctacagccgc gaaatccgca gtgtagcgcc gttggcacca cacgcgggcg agtgggggtg
     3121 cgccttagtg cttgccttct gtcttggcgt cgtgcttgcg gttcaatgca cggtagccga
     3181 tgtcgcggcg atattgcatg ccgtcgaacg agatctggtt gatcgtctcg tacgcggccg
     3241 actgcgcgct gcgcaccgaa tccgccagac cgaccacgca caacacgcgg ccgcccgagg
     3301 tggtcagctt gccgtccgcg agcgtggtgc cggcgtggaa cgtgaccgaa tcatcggtct
     3361 cggccggaat ctcactgatc cggtcgccct tcctcggcgt gtccggatag ttgtgcgccg
     3421 cgagcacgac gcccagcgcg gtgcggcggt cccattccag ttcgatcgtg ttcagcgtgc
     3481 cggcaatcgc ctgctcgacc accttcgaga agtcgccttt caggcgcgcc atgattggct
     3541 gcgtttccgg gtcgcccatc cggcagttga attcgagcgt cttcgggttg ccttgtgcgt
     3601 cgatcatcag accggcgtac aggaagccgg tgaagcggat gccttctttc tccatgccac
     3661 gcacggtcgg ctggatgatc tcgcgcatca cgcgtgcatg cagttgcggc gtgacgatcg
     3721 gcgcgggcga gtaggcgccc atgccacccg tgttcgggcc ctgatcggcg tcgagcagac
     3781 gcttgtggtc ctggctcgag gcgagcgcca gcacgtgctt gccatccacc atcacaatga
     3841 aactcgcttc ctcgccggcg aggaattcct cgatcacgac acgcgcgccg gcatcgccca
     3901 gcttgttgtc cgacagcatc atgtcgaccg cggcgtgcgc ttcctccagc gtttgcgcga
     3961 ccaccacgcc cttgccggct gcgaggccgt cggccttgat cacgatcggc gcgcccttgg
     4021 cgtccagata cgcgtgcgcg gcggccacgt cggcgaaggt ttcgtattcg gcggtcggga
     4081 tcgcatggcg cttcatgaac gccttggcaa aatccttcga gctttcgagc tgcgcggctt
     4141 ccttgctcgg tccgaaaatc ttcagaccgc gcgagcggaa caggttgacg atacccgcgg
     4201 cgagcggacc ttccggcccg accagcgtga aggcgacctg ttctttctcg acgaaatcgg
     4261 cgagcgcggc aggatcggtg atgtcgatgt tgcgcagacg ctcgtcctgg gccgtgccgc
     4321 cgttgccggg cgcaacgtag acgagctgga cccgcggcga ttgcgcgagc ttccatgcga
     4381 gcgcatgttc gcgaccgccg gaaccgacga cgagtaactt catgtgaatc cccgagactt
     4441 aaaaaccata aacggctgaa gcatcacgct cagccgtgga ataacagcga ggtttgcaat
     4501 gggcccgcag ccagagagcg gcgcagggca catagcgaac ccgggtggca acggtgcgga
     4561 agggcataaa ccaatgcccg ctcagtagcc gcgaggccta ccggaggcgc gtgcgtgatt
     4621 caatgccgat ccacgcgcaa agcgtcagat acagcgcgcc gccgggcacg ctcattcgtc
     4681 ggcgatcgcg gcgtttgtat agacttcctg cacgtcgtcc agactctcca gcgcgtcgag
     4741 cagcttctgc attttcaccg cttcgtcgcc ggtgaattcg acttccgtct gaggtttcat
     4801 cgtcacctcg gccagttcgg ccttgaagcc cgcggcttcg agcgcggtct tcaccttcgg
     4861 gaaatcgttc ggcgggcaca gcacttcgat actgccgtct tcattcgtga cgacgtcgtc
     4921 agcgccggct tcgagcgcgg cttccatcag cttgtcttcc ggcgtgccgg gcgcgaacag
     4981 gaactggccg acgtgatcga acatgaacga caccgagccg tccgtgccca tgttgccgcc
     5041 gttcttcgag aacgcatgac gcacgtccgc caccgtacgg gtgcggttgt cggtcatggt
     5101 gtccacgatc accgccgcgc cgccgatgcc gtagccttcg tagcggattt cttcgtagtt
     5161 tgcgccgtcc acgccgccca caccgcgctg gatcgcgcgg ttgacgttat ctttcggcat
     5221 gttggcgtcg tacgcctttt cgacggcgag ccgcagacgc ggattcgagt cgatgtcgcc
     5281 gccacccatg cgggccgcga cctggatttc cttgatgagc cgcgtccaga ccttgccgcg
     5341 cttggcgtcg gccgctgctt tcttatgctt gatgttggcc catttcgaat gacccgccat
     5401 acctttctcc atcgcgcgcg ccagcggggc gcacgcatag tgcaattgtc gttgcgatgt
     5461 cgggcgcctg cgactgcgat cctgattgcg atcttgcgcc tcgattcaga cagtttcagc
     5521 ttgataacgc ccggctgtgt ccggttgcgc cgcggctagc gggaccggtt gcagcgcggt
     5581 tggggccagg cggtgcggct agcgggctga aagcgccgcg ccagttccgg caagccccgc
     5641 gtaaaggcgc gatgcgccag gcagttgtgc aagttgagcc gctctttgag cggattcgaa
     5701 ttttatcacg gcgcgggggt gcggcagagg ctggatgagg cgaaagtagg cggcgcgcgc
     5761 gtcttttgga ccggccggac gcccggctcc gtgcccgaat gtcatcgccg ggcacggggc
     5821 cggacttgtt gaaataaaca tttttgttta tattagatcc tgtgttcatg aacacaggag
     5881 ggccggtgaa gcaaagcgag ttcagacgtt ggctcgctga ccaaggggcg actttcgaac
     5941 aaggcagcaa acatttgaag gtgtatctga acggtcggca gaccacactg ccgaggcatc
     6001 ccgctcacga aatttcggag ccgcttcgca aagcaattct caaacaactg aaatgcaaat
     6061 agaggcaagc cccgtaaggg gcttcatcgc cgcccaggct tttcccgtca agggaccacg
     6121 gagagttacc tatgtttcgc tatcccgccc gcatcgaacc agacgaagcc ggctttaccg
     6181 tttcctttcg cgacattcct gaggccctga cctcgggcgc gacgctggaa gaggcacgcg
     6241 aaatggcggc tgacgccctg gcgacggcga tggattttta ctttgaagac aaacgtcagg
     6301 tgccgttgcc atcaaaggcg cggcgtggcg aagaactgat cgaattgcct gccagcctcg
     6361 cggcgaaggt gctgctgctc aacgaaatgc tggcgcaaag agtgacggcc gcggaactgg
     6421 cgaggcgtct tcatacccgt gcacaagata tccagcgcgt gatcgatctc ggtcacgtaa
     6481 cgaagatcga cacgattgcg tccgcgctcg ctgctctcgg caaacgcctc gagttgtcga
     6541 ttgtgcccgc ataggcctct gcccgcgtag cgggcgcagg cacggcggcg ttgctcggtg
     6601 tcgtccgcgt ccgtgcgccg tctttccggg ctgcggtcag tttttcgtgc cgaacaggcg
     6661 gtcgccggcg tcgccgagac ccggcacgat gtacgcgtgc tcattcaggt gcgagtcgag
     6721 cgaggccacg tacaacttga cgtccggatg cgcgtcctgg aacacctgca cgccttccgg
     6781 cgcggccacc agcgcgagga acatgatgtt ctcgcccgcc acgttgcggc gcttgagcac
     6841 gtctaccgcg tgcacagccg agtaaccggt cgcgaccatc ggatcgcaca gaatgaacac
     6901 gcggtcttcc agatccggca ggcgcaccag atactcgacc gggcgatggt cctcggcgcg
     6961 atacacgccg atgtggccca cgcgcgccga cggcaccagc tccagcaggc cgtccgacat
     7021 gccgatgccg gcccgcagca ccggcacgat cgccagtttc ttgccggcga tcaccggcgc
     7081 gtcgatttcg accagcggcg tggtgaggcg gcgcgtggtc atcggcaggt tgcgggtgat
     7141 ctcgtagccc atcagcagcg tgatctcgcg cagcagttcg cggaacgtgc gagtcgaggt
     7201 gtcccggtcg cgcatatgcg acagcttgtg ctggatcagc gggtgatcga ggatgaaaag
     7261 attgggaaaa cggctgtcct gagtcatggc gggggcgcaa gcggcggaaa aagggatcgg
     7321 ctccggtatc ggatcggcgg ctggcgccgg ctgaaaatac ggccggcggg gcacgccacg
     7381 ccgcaagttt atcgaaagag tgccgccgga agataccgga gattgcagcg gcccggggca
     7441 gtccggcacc gattcttcta gaatcgggaa atgtggctgc gatcgggcag ccttcgtcgg
     7501 cgacacggcg acacggcgac acgggggcac gaccgtggcc acgaccaggg cacaaatggc
     7561 ccgcagccgg actacgagcg aatcacgaaa ggcgcgaccg ggccacgacc gggtcgcaat
     7621 cgaatcacaa caacgacgcg cacattcacg tcacgaggat acaaacatgg acatgggaat
     7681 cgcaggacgc accgcgctgg tttgcgcggc cagcaagggt ttggggcgcg gctgcgcgga
     7741 agcgctggca gccgaaggcg tcaacctgac gatcgtcgcg cgcacggcgg agacgctgga
     7801 ggcgaccgcc gccgaaatcc gcaagcaaag cggcgtggaa gtgaaaacag tggcatgcga
     7861 catcaccact ccggaaggac gcgccgcggc gctcgccgcg tgtccgcagc cggacattct
     7921 ggtcaacaac gcgggcggcc cgccgccggg cgacttccgc aacttcacgc acgaagactg
     7981 gatccgcgcg ctggagagca acatgctcac gccgatcgag ctgatccgcg cgaccatcga
     8041 cgggatgagc gagcgcggct tcgggcgcat cgtcaatatc acgagttccg ccgtgaaggc
     8101 gccgatcgac gtgctcggct tgtcgaacgg tgcgcgctcg ggtctgaccg gcttcatcgc
     8161 gggtctcgcg cgcagcaagg tggccggctc gggcgtcacg atcaacagcc tgttgccggg
     8221 catcttcgac accgaccgga tcgccaccac cttcgcggcg caagccaagg cgcaaaacat
     8281 ttccgtcgac gacgcgcgca agcagcgcat gaagacgatc cccgccggcc gtttcggcaa
     8341 tcgcgacgaa ttcggccgtg cctgtgcatt cctgtgcagc gtgcatgccg gctacatcac
     8401 cggccagaac tggctgatcg acggcggcac ttaccccggt acgttctgaa cggtaacgtt
     8461 tcgaggcaac ggcatcgctt tcactacaac aacgacaatc acaacggttt caggagtctc
     8521 acgtcatgag cacccgcatc gcgctaatcg cgcacgatca caagaaagac gacatcgtca
     8581 aactggccgg cgaatacgtc gatacgctca ggcgttgcga gatcgtcgcg accggcacga
     8641 ccggcacgcg catcggtgac gcgcacggtc tcacggtcga acgcatgctg tccggcccgc
     8701 atggcggcga cttgcagatc ggcgcgcagc tcgcggaagg gcgggtggac atggtgattt
     8761 tcctgcgcga cccgatgacc ccgcagccgc acgaaccgga tatcaacgcg ctggtgcgtg
     8821 cctgcgacgt tcacaacatt ccatgcgcca cgaacatttc gaccgcgcga atgattctcg
     8881 acgtgctgac cttgcgcctc acgcagcagg tttgaacgcg cgcatgttgg gcgatcaatc
     8941 gacgtcacac accagggaga cgtgatggca aaagcaatcc gattcgataa aaccggcggc
     9001 ccggaggtga tgaaatgggt cgacgtggag gtaggcgagc caggcgcggg cgagatccgc
     9061 atcaagcaga acgcggtcgg gctgaactat atcgacgtgt atttccgcac cggcctgtat
     9121 ccgctgccgc tgccgggtgg cctcggtatg gaggccgcgg gtgaagtcac cgcggtcggc
     9181 accggcgtga gcggtttcaa agcgggcgat cgcgtggcat acgtggcgcg tccgcctggc
     9241 gcctatgccc aggagcgcgt gctgtcggcg gcgcaagtcg tcaaggtgcc tgacgcgttg
     9301 agcgacgagc aggccgcttc tgtcatgctg caagggctga cggcgcagta tctgctgcgc
     9361 cgcacgtatc cggtgaaggc cggcgacacg atcctgatcc aggccgcggc cggcggcgtc
     9421 ggcctgctgg tgtgccaatg ggcgaaggca ttgggtgcga cagtgatcgg cacggtcggc
     9481 tccgatgaaa aggccgagat cgccaaagcg cacggctgcg accacgcgat cgtctacacg
     9541 cgcgaggact tcaccaggcg cgtgcgtgag atcaccaacg gcgcgggtgt gccggtggtg
     9601 tacgactcga tcggtaagga cacgtttacc ggctcgctcg attgcctcgc gccgctcggc
     9661 atgttcgtga gcttcggcaa tgcgtcggga ccgttgccgc cgatcgattc gtcggaattc
     9721 gccggacgcg gttcgctgtt ctttacgcgc ccgacgctct tcacctatat cgccaaacgc
     9781 agcgactacg aggcgatgtc cgccgagctg ttcgacgtgc tggtgtcggg caaggtgaag
     9841 acgagcatca accagcgtca tgcgctggcg gatgtcgggc gcgcacacgc ggatctcgaa
     9901 ggccgccgca ctacgggctc gacggttctg ctgccgtaag cgctcatgcc atgaacacac
     9961 ggcggcgcat cgcgcgggtg taaaaactgc cgcgccgcgg cgccgctgtg tgtggcatcc
    10021 aaggccggcg cgaaatgccg caagcccata ccccgtcgcc ataaggccgt tccgtctccg
    10081 atggaacggc cttttccgtt tacgcgattt ttacattgca tctcaaatct ttgacctgcc
    10141 ctttacgcgc ttcggcataa cgttgcacct atccaaagtc cgtgagtgga gaacaagatg
    10201 gatgtgctgt atgtcggggg gctggtgctg ttttccgcgc tgacctttgc attgattgcc
    10261 ggctgcgaga agttgatgca gttccggcgc ggtcagggag tgcggtcatg aactggattc
    10321 tctggttgtc gggcgcggct accgcgctgt tgtttgcgta tctggtctat gcgctgttgc
    10381 gcgcggagga cattgaatga actcgaacaa tgtcctgcaa gcgggcctct tcatcgtcgt
    10441 gctgctgtta gccgcggtgc cgatgtcccg ctatatgacc aatgtgatgg acggcacgtc
    10501 gcgcgtggtc cgctttggcg gcccgctcga acgcttgctg tatcgcattg cgggcgtcga
    10561 tccgtcgacg gaaatgagct ggaagcacta cgcgatcgcc acgatcgcgt tcaacgtgct
    10621 cggcgtactg ttcctttacg tactgttgcg cgtgcagggc tggttgccgg gcaatccgca
    10681 gcagttcggc ccgatgaccg tggacggcgc gttcaatacc gcggtcagct tcgtgaccaa
    10741 cacgaactgg caggattaca cgcctgaaca aaccgttggc tatctgacgc agatgctcgg
    10801 cctgaccgtg cagaacttct tctccgcggc cactggcatt gtggtggtga ttgcgctgat
    10861 tcgcggcttc gcgcgtcata ccgcgcagac catcggcaac ttctgggtcg acctcacgcg
    10921 tgtgacgctg tatgtgttgc tgccgacgtc ggtgatcgtc gctgcggtgt tgatgagcca
    10981 gggcgtgatc cagaacttca aggcgtatga agacgtgccg acgctgcaga ccacgaccta
    11041 cgccgcaccg aagctcgatg cacaaggcaa tcccgtgaag gatgccaagg gcaatgcagt
    11101 gacggtcgac acgcccgtga agacgcagac cctcgcgatg ggcccggttg cctcgcagga
    11161 agcgatcaag atgctcggca ccaacggtgg cggtttcttt aacggcaact cggcgcatcc
    11221 gtacgaaaac ccgacgccgt tctcgaactt cctgcagatc ttctcgatgc tgatcatccc
    11281 cgcgtcgctc gcgctggtgt tcggccggat gatcatggac cgcaagcagg gcgtggccgt
    11341 gctcgcgacg atggcgatcg ccttcggcgt gtgcgtgttc ggcgagatcg gcgccgagca
    11401 atcgggtaat ccggcgttga cggcgttgca tgtcgatcaa tcggcgactg cgttgcagtc
    11461 gggcggtaat gcggaaggca aggaaacgcg cttcggtatc gcgcagtcgg gcatcttcac
    11521 ggtggcgacc acggcggctt cgtgcggcgc ggtggccaac acgcacgact cgctgacgcc
    11581 gattggcggc ctctatccga tgctgttgat ccaactcggc gaagtgatct tcggcggcgt
    11641 gggttccggt atgtacggga tgctcgtgtt cgcgatgctg gcggtgttcg tggcgggcct
    11701 gatgatcggc cgcacgccgg aatacgtcgg caagaagatc gaagcgtatg aaatgaagat
    11761 ggtgtcgatc gtggtgctgc tcacgccgct gctggtgctg gtgggtacgt cgatcgcggt
    11821 gctgaccgat gccggcaagg cgggtatcgc caaccccggc gcgcacggtt tctccgaaat
    11881 cctctacgcg ttcagctcgg ctgcgaacaa caacggtagt gcgtttgctg gtctgacggt
    11941 gagcacgccg ttctacaact ggctgacggc gatcgcgatg tggttcggcc gcttcggcac
    12001 ggtcatcccg gtgctggcga ttgcgggctc gctcgcggcc aagaagcgca ttggtgtgac
    12061 cggcggcacg ctgccgactc atggcccgct gttcgtcgtg ctgctgctcg gcacggtgct
    12121 gctggtcggc gcgctgactt atgtgcctgc gctcgcgctc ggtcctggtg tcgaacatct
    12181 gatcatgatg ggcgcccatt gaggcggccc gtcgagatga cgcaagacca gtgagtgcac
    12241 aggcataagt gacgcgtaac gggaaattcg aggattcaat gactgaacat aacgcaacca
    12301 ggtccatgtt cgacccggcg ctgctgcgcc cggcgatcgt ggactccttc aaaaagctca
    12361 cgccgcgcac gcagttccgc aatccggtga tgttctgcgt gtacgtcggc agcattctga
    12421 cgaccattct gtggatcgcc gcgcttggcg gccaggccga ggcgcccgcg ggtttcattc
    12481 tcgcggtcac cttgtggctg tggttcacgg tgctgttcgc gaacttcgcg gaggcgcttg
    12541 ccgaaggccg ttccaaggcc caggccgcgt cgctgcgcag cgcgaagaaa gacgtgatgg
    12601 ccaagaagct caacgagccg catccgaagt cgccgatccg tattctgacg gcgtccgatc
    12661 tgcgtaaagg cgacgtcgtg ctggtcgaaa cgggcgacgt gattccggcc gacggtgaag
    12721 tgatcgacgg tgtcgcgtcg gtcgacgagt cggcgatcac gggtgaatcc gcaccggtga
    12781 tccgcgaatc gggcggcgac ttttcgtcgg tgacgggcgg cacgcgcgtg ctgtcggact
    12841 ggatcgtcgt gcgggtgacg gccaatccgg gcgaagcctt cctcgaccgc atgatcgcca
    12901 tggtcgaagg cgcgaagcgt cagaagacgc cgaacgaaat cgcgctgacg attctgctgg
    12961 tcgcgctgac catcgtgatg ctgctcgcca cggcgacgct gctgccgttc tcgatgtttt
    13021 cggtcgaagc cgcaaaagcg ggccacgtgg tgacgattac cgcgctggtc gcgctgctgg
    13081 tgtgtctgat tccgaccacc atcggcggac tgttgtcggc gatcggtgtg gccggtatga
    13141 gccgcatgat gcaagcgaac gtgatcgcca cctcgggccg cgcggtggaa gccgctggcg
    13201 acgtcgacgt gctgttactc gacaagaccg gcacgatcac gctcggcaac cgccaggcat
    13261 cgcagttcgt gccggcaccg ggcctgaccg aagaagcgct ggcggacgcc gcccagcttt
    13321 cgtcgctcgc cgacgaaacg ccggaaggcc gcagcatcgt cgtgctggcc aagcagcgtt
    13381 tcaacatccg tcaacgcgat atggcttcgc tgcacgcggt gttcctcgcg ttcacggcgc
    13441 aaacgcggat gagcggtgtc gatttgccgg gccgtgaaat ccgcaagggc gcctccgatg
    13501 cagtgaagca ctacgtcgaa gcgaatggcg gccgtttccc gaacgaagtc agcgcggcag
    13561 tggccgaagt cgcacgtcgc ggtagcacgc cgctggtggt cgccgagaaa ggcgagccgg
    13621 gcgcacgcgt gcttggcgtg atcgaactga aggacgtggt gaatggcggc atcaaggaac
    13681 gctttgccga actgcgcaag atgggtatca agaccatcat ggtgaccggc gacaaccggc
    13741 tcaccgccgc ggcgatcgca gcagaagccg gcgtcgacga tttcctcgcc gaagccacgc
    13801 cggaagcgaa gctcgcgacg attcgcgcgc accaggcgga aggtcgtctg gtcgcgatga
    13861 ccggcgacgg caccaacgac gccccggcgc tcgcccaggc cgacgtcgcg gtggcgatga
    13921 ataccggcac gcaggccgcg aaagaagctg gcaacatggt cgacctcgat tcgaatccga
    13981 ccaagctgat cgagatcgtc gagatcggca agcagatgct gatgacgcgc ggctcgctca
    14041 ccacgttctc gattgccaac gacgtcgcca agtacttcgc gattattccg gctgcgttcg
    14101 cgaccaccta tccggcgctg aacgcgctga acgtgatgca tctggccacg ccggcctcgg
    14161 cgatcatgtc ggcggtgatt ttcaatgcgc tgatcatcgt gctgctgatt ccgctggc
//
LOCUS       DATASH010000392         2237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4413261,
            whole genome shotgun sequence.
ACCESSION   DATASH010000392 DATASH010000000
VERSION     DATASH010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4413261"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="VHV99_11010"
     CDS             <1..741
                     /locus_tag="VHV99_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012404609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3636003.1"
                     /translation="KLHIEPLLDRRPGELSGGQQQRCALARALVKRSSLVLLDEPLVN
                     LDYKLREELRVELSTLFADGKTTVVYATTEPLEALLLGGYTAIVDKGRVLQYGPTLDV
                     YNAPVNVDAAAVFNDPPMNMLTSELTGNGSARLPIGIDVPISRAAAETGTCRIGIRPG
                     HLRLAPRTPKSIAVPCRLELAELSGSETYLHLRTRDGGIDLVAQLQGVHQIGLGTQLD
                     VFIDPDELFVFGADTKLVSSPEAAYGAH"
     gene            728..1840
                     /locus_tag="VHV99_11015"
     CDS             728..1840
                     /locus_tag="VHV99_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016347370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3636004.1"
                     /translation="MARIEFQNLAHAYRPNPATLNDYALQPMSMVWEDGGAYALLGPS
                     GCGKTTLLNIVSGLVTPSEGKVLFNGHDMTAHGARERNIAQVFQFPVIYDTMTVFDNL
                     AFALRNRKVAEGEVKKRVHEVAEILDMTRELPRKASNLAADAKQKISLGRGLVRKDVA
                     AILFDEPLTVIDPNMKWMLRRQLKKIHQQLKLTLIYVTHDQVEALTFADEVVVMTNGR
                     IVQKGGPEALFLRPDHAFVGYFIGSPGMNLCPVELDTDGVTVGSQRVALDVDTLATLR
                     HANGALTLGIRPEFVRLAAVGEAGAVKAQLLRAQQLGNYQLVTAQCDGHVFKAKIEPH
                     LRVSDGPVWLRLAAPETVFFSNDERIAISITERTSR"
     gene            1837..>2237
                     /locus_tag="VHV99_11020"
     CDS             1837..>2237
                     /locus_tag="VHV99_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX3636005.1"
                     /translation="MNKPVNQKAWLLVVPVFICVAFSAILPLMTVVNYSVQDIISPTQ
                     HVFVGTEWFRNIMTDPDLRSALGRQIIFSTCVLLFEIPLGVGLALAMPASGWRASMSL
                     VVLAMPLLIPWNVVGTIWQIFGRPDIGLLGYV"
BASE COUNT          439 a          683 c          683 g          432 t
ORIGIN      
        1 aaactgcata tcgaacctct gttggaccgg cgtccaggcg agttatcggg cggccagcaa
       61 cagcgctgcg cactcgcgcg tgcgctcgtg aagcgctcgt cactggtgct gctcgacgag
      121 ccgctggtga acctcgacta caagctgcgc gaggaattgc gtgtcgagtt gagcacgcta
      181 ttcgcagacg gcaagaccac ggtcgtctac gcgaccaccg aaccgctcga agcgttgctg
      241 ctcggcggct acacggcgat cgtcgacaaa ggccgcgtgc tgcaatacgg tccgacgctc
      301 gatgtctaca acgcaccggt gaacgtcgat gcagcggctg tcttcaacga cccgccgatg
      361 aacatgctga ctagcgagct gacaggaaac ggcagcgccc gcctgccgat cggcatcgac
      421 gtaccgatca gtcgcgcggc ggcagaaacc ggcacatgcc gcatcggcat tcgccccggg
      481 catctgcgcc tggcgccacg cacgccgaaa tcgatcgccg ttccatgccg gctcgaactg
      541 gccgaactga gcggctcaga aacctatctg catctgcgta cgcgggatgg cggcatcgat
      601 ctcgtcgcgc agttgcaagg cgtacatcag attgggctcg gcacgcaact cgatgtattc
      661 atcgaccctg atgaactgtt tgtattcggc gcggacacga aactggtatc cagcccggag
      721 gcggcttatg gcgcgcattg agtttcagaa tctcgcacac gcgtatcgtc cgaatccggc
      781 gaccctcaac gactacgcac tgcaacccat gagcatggtc tgggaggacg gaggcgccta
      841 tgcgttgctc ggtccgtccg gttgcggcaa gacgacattg ctgaatatcg tctcgggtct
      901 cgtcacgccg tccgaaggca aggtgctgtt caacggacac gacatgaccg cgcacggcgc
      961 gcgagaacgc aatatcgcac aggtgttcca gttccctgtg atctacgaca cgatgaccgt
     1021 gttcgataac cttgcgttcg cgctgcgcaa ccggaaggtc gctgagggcg aagtgaaaaa
     1081 gcgcgtgcac gaagttgcgg aaatcctcga catgacgcgc gagctgccgc gcaaggcaag
     1141 caatctagcg gcggatgcga agcagaagat ttcgctaggg cgcggcctcg tgcgcaagga
     1201 tgtcgcagcg atcctgttcg acgaaccgct gaccgtgatc gatccgaaca tgaagtggat
     1261 gctgcgccgc caactgaaga agattcacca gcaactcaag ctgacgctca tctatgtcac
     1321 gcacgatcag gtcgaagcgc tgacctttgc cgacgaagtc gtcgtcatga cgaacggccg
     1381 catcgtgcag aagggcggac cggaagcgct gtttttgcgt ccggatcatg cgttcgtcgg
     1441 ctatttcatc ggcagtcccg gaatgaatct ctgcccggtc gaactcgata cggacggcgt
     1501 cacggtcggc tctcagcgcg tggcgctcga tgtcgacacg ctcgcgacgc tgaggcacgc
     1561 gaacggtgcg ctgaccctgg gcattcgccc cgagttcgtg cggctcgcgg ccgttggcga
     1621 agcgggcgcc gtcaaggcgc aactgttgcg cgcgcaacag ctcggcaact atcagctcgt
     1681 caccgctcaa tgcgacggcc atgtgttcaa ggcgaagatc gagccgcatc tgcgggtgag
     1741 cgatggaccc gtctggctac gacttgccgc gcccgagacg gtgttcttca gcaatgacga
     1801 aagaatcgcc ataagcatca ccgagaggac ttcgcgatga acaagcccgt caatcagaaa
     1861 gcgtggctgc tggtcgtgcc ggttttcatt tgcgtcgcgt tctccgcgat cctgccgctt
     1921 atgacggtgg tcaactattc ggtgcaggac atcatcagcc cgacgcagca tgtctttgtc
     1981 ggcaccgagt ggtttcgcaa catcatgacc gatccggatc ttcgcagcgc gcttggcaga
     2041 cagatcattt tctccacgtg cgtgttgttg ttcgagatac cgctcggcgt cggcctcgcg
     2101 cttgcaatgc ctgcgtccgg atggcgcgcg tcgatgtctc tggtcgtgct cgcgatgccg
     2161 ctgctgattc catggaacgt ggtcggcacc atctggcaga tttttggccg gcccgacatc
     2221 ggcctgctcg gctatgt
//
LOCUS       DATASH010000393         2261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2236971,
            whole genome shotgun sequence.
ACCESSION   DATASH010000393 DATASH010000000
VERSION     DATASH010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2236971"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1376)
                     /locus_tag="VHV99_11025"
     CDS             complement(<1..1376)
                     /locus_tag="VHV99_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3636006.1"
                     /translation="MRNVEMADASCGPLSIRLLGELELRRDGVGVPLPASRRARALLG
                     YLVLSGGAIPRSALCDLLWDGPDDPRASLRWSLSKLRPLVDDASHKRIVASRTHVAFD
                     RAGAFIDATRILALLAPGTERTPLAALEEAAQLLRGDFLAGIELPNCYRFHNWCVGQR
                     ERFGRIRGTVLRTLVESLAPDVPLALQYGRELVDADPLDESGHATLIGLLEAAGRHPD
                     AQEHHRYARELLQRELGARACAMLDEAVRRMRQNACLAQTNDCSYRAHGDSNRPPEDG
                     PHGAVQSEPARTGNDETVWRLPLIGRDAERAKLDVLLASPSAAKQLTLLTGEPGIGKT
                     RLLEYFSQCASNAAYRVLRGRCYEAETVRPYGIWIDALGDFALDALRPVDETLAPLVR
                     ATVSSTSEIQREERGRERFFEAIVALLGRLSVEHRLAFLFDDLQWLDESSAALLHFVL
                     RRLDRQLPV"
     gene            1450..1863
                     /locus_tag="VHV99_11030"
     CDS             1450..1863
                     /locus_tag="VHV99_11030"
                     /inference="COORDINATES: protein motif:HMM:NF044766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636007.1"
                     /translation="MNADYYQSGVLCYLKLQEANVAFYQSKLSDYLGVLVHRRGALRA
                     GTTLAEADAVRLYETLAHHPTCAQRLAKKTGTDPAAVREWLGSQVTRGHLQYDAALQR
                     YWMTEQQAFVIGREPGLCFVPDAFRVWRRSGPFAR"
BASE COUNT          446 a          700 c          717 g          398 t
ORIGIN      
        1 accggcagtt ggcggtccag ccgtcgaagg acgaagtgca gcagcgccgc ggatgattcg
       61 tcgagccact gcagatcgtc aaagaggaag gccaatcgat gttcgacaga cagacggcca
      121 agcagcgcga caatcgcttc gaaaaaccgc tcgcggcctc gctcttcgcg ctggatttca
      181 gacgtgctgc tgacagtcgc acggacgagc ggcgcgagag tttcgtcgac gggccgtaat
      241 gcgtcgagcg cgaagtcgcc cagtgcgtct atccagatgc cgtaagggcg gacagtctcg
      301 gcttcatagc agcgaccgcg aagcacgcgg tacgccgcat tcgacgcgca ttggctgaag
      361 tattctagaa gccgcgtctt gccgataccc ggctcacccg tcagcaaagt cagttgtttg
      421 gctgcggacg gagaagccaa caggacatcg agcttcgcgc gttccgcgtc gcgcccgatc
      481 agaggcaaac gccagaccgt ttcgtcattg cccgttcgcg ctggctcgct ttgcacggct
      541 ccatgcgggc cgtcttcagg cgggcggttc gaatcgccat gtgcgcgata ggaacagtcg
      601 ttcgtctgag ccaagcaagc attttggcgc atgcgccgaa cagcctcatc gagcattgca
      661 catgcgcgtg cgcccagctc gcgttgaaga agctcgcgcg cgtagcggtg atgctcctgc
      721 gcatccggat gacggccagc ggcttccagc agcccgatca gcgttgcgtg gcccgattca
      781 tccagcgggt cggcgtccac cagttcccgg ccgtactgca aggcaagcgg cacatcaggc
      841 gccaggcttt cgacgagcgt gcgcagaacc gttccgcgaa tgcgtccgaa tcgttcgcgc
      901 tggcccacac accagttgtg gaaacggtag caattaggaa gctcgatccc ggcaagaaag
      961 tcaccccgca gcaactgcgc tgcttcctcc agcgctgcaa gcggcgtccg ctcagttcct
     1021 ggtgcgagca gcgccagaat cctcgttgca tcgatgaatg cgccggcgcg atcgaacgca
     1081 acgtgcgtcc ggctcgccac gatgcgcttg tgcgatgcgt catcgaccag aggccgcagc
     1141 ttggacaggc tccagcgaag cgaggcgcgc ggatcgtcgg gaccatccca tagcaggtcg
     1201 cacagggccg atctcggtat tgcgccgccg gacaggacga gatagcccag cagcgcgcgc
     1261 gcgcggcgcg aagcgggcaa gggcactccc acgccatcgc gcctcagttc gagttcgcca
     1321 agcaggcgaa tgctcaacgg tccgcacgaa gcgtcagcca tctcgacgtt tctcacgctg
     1381 gctccaaccg tcaatacaac gtttcaggcg cacgctcatt tcgtcaaacg gcactgatcg
     1441 gatggtcgtt tgaacgcaga ttactaccag agcggagtgc tttgctacct gaaattacag
     1501 gaggccaacg tggctttcta ccaaagcaag cttagtgact acctcggcgt actggtgcat
     1561 cgtcgcgggg cattgcgggc gggcacgacg ttagccgaag cggacgcggt ccggctatat
     1621 gaaacattgg ctcaccatcc cacctgtgcc cagcgacttg cgaagaagac ggggacggat
     1681 ccggcggcag tgcgtgagtg gttggggtcg caggtgacgc gcggtcacct ccagtatgac
     1741 gcggcgcttc agcgatactg gatgaccgaa caacaggcgt tcgtcatcgg ccgcgagccc
     1801 gggctctgtt tcgtccccga cgcttttcgg gtctggcgcc gcagcgggcc gttcgcgcgg
     1861 tgatgcaggc atacggcggc aggacgttca cccgccgctg aacaccgttt cgggcgaaag
     1921 gcgaaagacg cacagccgtg tgggtgaggt aattaaacgg cctgatgatt cgccacgcgc
     1981 cagtgggtga cgcgatctcg tgtgccagcg aaaatggaca gcattccgcg cttgcgcaca
     2041 cgtcgacaga ttgtcgcaaa gcgcgcctag agcgcctgac cattgggtgt ttgagaacgg
     2101 tcattgccgg catagcgatg cgaaagacgg caacgcttaa cggtttcggg cgactgcgtc
     2161 gggcagtgac cggccaatgc gcgttgttcg acggtccgcc agtctggttc cggctgcatc
     2221 cctaagcggt cactcacgga tcggcgccgg ccaaatcaaa g
//
LOCUS       DATASH010000394         6533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2434714,
            whole genome shotgun sequence.
ACCESSION   DATASH010000394 DATASH010000000
VERSION     DATASH010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6533
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2434714"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..946)
                     /gene="nadC"
                     /locus_tag="VHV99_11035"
     CDS             complement(47..946)
                     /gene="nadC"
                     /locus_tag="VHV99_11035"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEX3636008.1"
                     /translation="MGVVERNQLEAVSPLFAEIHAQYGVALDAALARNVADTLAEDVG
                     AGDQTGRLVPADEVRDARIIVREDAVLCGVPWFNAVMHQIDPRIELQWRYREGDRMTA
                     NTPVCELRGPVRALLTAERNALNFLQLLSGVASATRRYVDAIAHTQTRILDTRKTLPG
                     LRLAQKYAVRVGGGANQRLALYDGILIKENHIAAAGGVGAAMDAALALNAGVSIQIEV
                     ETLAQLETALAHRAQSILLDNFSFDAMRDAVRITAGRAVLEVYGGVNFETVRTIAETG
                     VDRVSIGALTKDVRATDYSMRIV"
     gene            complement(946..2085)
                     /gene="nadA"
                     /locus_tag="VHV99_11040"
     CDS             complement(946..2085)
                     /gene="nadA"
                     /locus_tag="VHV99_11040"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEX3636009.1"
                     /translation="MDQQAIRTVEYDRPQAQGTTCGIGQAWAKVPDAPSADQRVALKE
                     RIRALLKREKAVLVAHYYVDAELQELADETGGCVADSLEMARFGRDHEAQTLVVAGVR
                     FMGETAKILSPNKRILMPDLDATCSLDLGCPVDEFSAFCDAHPDRTVVVYANTSAAVK
                     ARADWMVTSSIGLEIVADLHARGEKIIWAPDRHLGSYIRSKTGADMLLWQGSCLVHDE
                     FKGIELDLLRAEYPGAKVLVHPESPANVVAQADVVGSTTQLIDAAQKLDATHFIVATD
                     LGILHKMQLAAPGKTLIAAPTAGNSATCKSCAHCPWMAMNGLANLADVLERGHNEIFV
                     DQAIGERARLPIDRMLDFAARHKKRVQASGDFARDAQLYSNVGAA"
     gene            complement(2229..3425)
                     /locus_tag="VHV99_11045"
     CDS             complement(2229..3425)
                     /locus_tag="VHV99_11045"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX3636010.1"
                     /translation="MLNSLLDFLAHGVLHFSWWQLVLYTLVATHITIIGVTVYLHRCQ
                     AHRALELHPAVSHFFRFWLWMTTGMLTGQWAAIHRKHHAKCETEEDPHSPQTRGIWKV
                     LLEGAELYRSEAKNEETMRKFSHGTPNDWMERNVYTKYPILGVSLMMVLNVALFGVVG
                     LTIWAVQMVWIPFWAAGVVNGLAHWWGYRNFNSSDASTNIFPWGILIGGEELHNNHHT
                     YATSAKLSNKWYEFDIGWMYIRIMSAFRLAKVKKIAPTPRLTTGKLVLDQDTLQAVLA
                     NRYEVMARYGKALKRAYRQELAHLKEVGAREKYQVMRGARSWFHKEEAGLDEPQKRQL
                     PQIFANSQKLKTYIDMRNELAAMWERSNASRDQLLIQLQDWCHRAEQSGIKALQEFAL
                     RLRRYA"
     gene            complement(3629..4984)
                     /locus_tag="VHV99_11050"
     CDS             complement(3629..4984)
                     /locus_tag="VHV99_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEX3636011.1"
                     /translation="MQNRIFPHMLGDLARDFGQPVMLWQVGVLVGTLIIAWLLARLLR
                     RALDLRRQTRYQTLRFGAESLNRAFFPLIGAALVWLARAITGQFMHTALLDLALVPLF
                     GIGLIYIVFFFARRVFSQGGETHPWLFLVEKIVSLVVWIGMVLTVMGIQDDVIAWMGS
                     VHFRVANAHMTLLSLITGLLWVCVTMIVAMWLGSAFEDRLIRSTTLDANLKVVVARVG
                     RAVFVLAAVLISLSLVGIDITVLGVFGGALGVGLGFGLQKIASNYVSGFIILIDRSLR
                     IGDTINVSGLQGMVTQIRTRYTVVRGLDGIETLIPNEKLITDVVQNQSSYLTRGYAKL
                     AVQIAYTSDVEQAMALLAQAAQGVARVLQEPAPTAYLASFGPDGINLELGFWIEDAAT
                     GTSSVRSTVNRNIWRLFSEHDISIPFAQREVRIVGNMSDAMPQAVAMTDSRANPADSA
                     R"
     gene            complement(5057..6316)
                     /locus_tag="VHV99_11055"
     CDS             complement(5057..6316)
                     /locus_tag="VHV99_11055"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEX3636012.1"
                     /translation="MRLHGFLIGQTETLLAEVLKLNGPADATTSRFFRAHPKLGHGER
                     GVIAEAVFAVLRRRMEFAHLAEGGAGSPARRMALLGLMQTAGRTALKPFVSDTESHWL
                     EHVAKIDPESLPLRTRLNLPDWIYQALSRRFEAEELAQLAAALNYPAPLDLRANPIKA
                     SRDDVLNALSKAGIEGGATPFAPFGVRVVGKPPLTKLDAFQHGWVEVQDEGSQLLCSL
                     VAPKRGEMIVDFCAGAGGKTLALGAAMRSTGRLYAFDISERRLAKLKPRLARSGLSNV
                     NPVLIDSEHDAKIKRLAGKIDRVLVDAPCSGLGTLRRNPDLKWRQSPESVAELAPKQA
                     SILASASRLVKKGGRLVYATCSILEAENEAVVQQFLADHPDFALVPARDVLAEQRIEL
                     EMGDYLSLWPHRHATDGFFAAVLERQS"
     gene            complement(6313..>6533)
                     /locus_tag="VHV99_11060"
     CDS             complement(6313..>6533)
                     /locus_tag="VHV99_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX3636013.1"
                     /translation="HGPIVAQAAVPVEAGDTPAMLAERVLATEHIIYPRAVRWFVDGR
                     LALDGLRVPLTPPEPQWLFAGHTAGEGA"
BASE COUNT         1297 a         2112 c         1917 g         1207 t
ORIGIN      
        1 ccggcgatgg cctttgtcat tgatcgctac cgctaaagcg taagcgtcaa acgatccgca
       61 tcgagtagtc tgttgcgcgc acgtctttgg tcagcgcccc gatcgacacg cgatcgacgc
      121 ccgtttccgc gatggtccgc accgtctcga agttcacacc gccatacact tccagcaccg
      181 cccgccccgc cgtgatacgc actgcgtcgc gcatcgcgtc gaacgaaaag ttgtcgagca
      241 gaatggattg cgcgcgatgc gccaatgcgg tttctaattg ggccagcgtt tcgacttcga
      301 tctggatcga cacccccgca ttcagcgcaa gcgccgcatc cattgccgcg ccaacgccgc
      361 cggctgcggc gatgtggttt tccttgatca gaatgccgtc gtacaacgcg agccgctgat
      421 ttgcgccgcc gcccacgcgc accgcgtatt tctgcgcgag acgcaaaccc ggcaaagtct
      481 tacgcgtatc gaggatacgc gtttgcgtgt gagcaattgc atcgacgtag cgacgcgttg
      541 cgctcgccac gcccgacagc aactggagga agttcagcgc attgcgttca gcagtcaaca
      601 gcgcgcggac aggaccgcgc agctcgcaga ccggcgtatt cgctgtcatc cgatcaccct
      661 cgcgataccg ccactgcagc tcgatacgcg gatcgatctg atgcatcacc gcgttgaacc
      721 aaggcacacc gcacagcaca gcgtcttcac gcacgatgat gcgtgcatcg cgcacctcgt
      781 ctgcgggaac aagacggccg gtctgatcgc ccgcgcccac gtcttcggcc aacgtatcgg
      841 cgacattgcg cgccaacgca gcatcgagtg ccacgccata ctgcgcatga atctccgcga
      901 acagcggcga caccgcctcg agctgattac gttccaccac ccccattacg ctgctcccac
      961 gtttgaatac agttgcgcat cgcgcgcgaa atcgccactt gcctgcacgc gcttcttatg
     1021 acgcgccgcg aagtccagca tccggtcgat cggcaggcgc gcccgctcgc cgatcgcttg
     1081 atcaacgaag atttcgttgt ggccgcgttc gagcacgtcg gccagattgg cgaggccatt
     1141 catcgccatc cacgggcaat gcgcgcagct cttgcaggtc gcgctgttgc ccgccgtcgg
     1201 cgcggcgatc aacgtcttgc cgggtgcggc gagctgcatc ttgtgcagaa tgccgagatc
     1261 ggtcgctaca atgaaatggg tcgcgtcgag tttttgcgcg gcgtcgatca gctgggttgt
     1321 cgaaccaacc acgtccgctt gcgcaaccac attagccggt gactccggat gcaccagcac
     1381 cttcgcgccc ggatattcgg cgcgcagcag atcgagttca atgcccttga attcgtcgtg
     1441 cacgagacac gagccctgcc acagcagcat gtccgcaccg gtcttgctcc ggatatagct
     1501 gccgagatga cgatccggcg cccagataat cttttcgcca cgtgcgtgca aatcggcaac
     1561 gatctccagt ccgatcgacg acgtcaccat ccagtctgcc cgcgccttga cggccgcgct
     1621 ggtgttcgcg tacacgacca ccgtgcgatc cgggtgagcg tcgcagaaag ctgagaattc
     1681 atcgaccgga cagcccaggt cgagcgaaca ggtcgcatcg agatcaggca tcagaatgcg
     1741 cttgttcgga ctcaggattt tcgcggtctc gcccatgaag cgcacgcccg ctaccaccag
     1801 cgtctgcgcc tcgtgatcgc ggccgaaacg cgccatctcc agcgagtcgg cgacgcagcc
     1861 accggtttcg tcggcgagct cctgaagctc cgcgtcgacg taatagtgcg caacgagcac
     1921 cgctttctcg cgcttaagca atgcgcggat gcgctccttc agcgccaccc tctggtccgc
     1981 cgacggcgcg tcgggcacct tcgcccaagc ctgcccgatc ccgcaggttg tgccttgtgc
     2041 ctgtggccgg tcgtattcga cggtcctgat cgcctgctga tccatgatct cctcttccct
     2101 tcgctctgcc gccgcggaca cttaatcgcg tgccgttgca atttccgcgg cccaaaaagc
     2161 aaaaccccgc caacgcgggg ttttgtgacg taacgaaatt ttaatagatt tcgcgctcag
     2221 gcatacgatc aagcataacg gcgcagacgc aacgcgaatt cctggagcgc tttgataccg
     2281 ctctgttcag cgcgatgaca ccagtcttgc aattgaatca gcaattgatc gcgcgacgca
     2341 ttcgaccgct cccacatcgc cgccagttca ttgcgcatgt cgatgtacgt tttgagcttc
     2401 tggctattcg cgaagatttg cggcaactgg cgcttctgcg gctcgtcgag acccgcctct
     2461 tccttgtgga accagctacg cgcaccgcgc attacctgat acttctcgcg cgcgccaact
     2521 tccttcagat gcgccaactc ctggcggtaa gcgcgcttga gcgccttgcc gtagcgcgcc
     2581 atcacttcgt agcggttggc cagcacggct tgcaacgtgt cctgatcgag caccagcttg
     2641 ccggtggtca aacgcggcgt aggtgcaatc ttcttcacct tggccaggcg gaacgccgac
     2701 atgatgcgga tgtacatcca gccgatgtcg aactcatacc atttgttcga cagcttggcc
     2761 gacgtagcat acgtgtggtg attgttatgc aactcttcgc cgccgatgag gatgccccat
     2821 ggaaaaatgt tggtgctggc atccgacgag ttgaaattgc ggtagcccca ccagtgcgcg
     2881 agaccattca ccacgccggc tgcccagaac gggatccaca ccatctgcac ggcccagatc
     2941 gtcaggccaa ccacaccgaa caacgcgacg ttgagcacca tcatcaggct cacgccgagg
     3001 atcgggtact tggtgtagac attgcgttcc atccagtcat tcggcgtacc gtgactgaac
     3061 ttgcgcatgg tttcttcatt cttggcttcg gaacggtaca gttccgcgcc ttccagcagc
     3121 accttccaga tgccgcgcgt ttgcgggctg tgcggatctt cttcggtctc gcacttggcg
     3181 tggtgcttac ggtgaatcgc ggcccattgg ccggtcagca tgccggtggt catccacagc
     3241 cagaaacgga aaaaatgact gacggccgga tgcaactcca gcgcgcggtg cgcctggcag
     3301 cgatgcaaat agaccgtcac gccgatgatc gtgatgtgtg tcgcgacgag cgtgtacagc
     3361 acaagttgcc accacgagaa gtgcagcacg ccatgggcaa ggaaatcgag caaggaattc
     3421 aacaaggcaa tttacctgtg gaacaagaga cgccagacac acgcaaaatg cgcgtgtcaa
     3481 caaagtgaaa gcatacagcg ggattggaag atattctact tgaagcattc caagtgtttg
     3541 taacaaatgg agattctttg tggggtatca accggaagga cctcaccgca gaccttttta
     3601 gaccttctgc gggcgttcga agtgcccgtc agcgggcgct atcggctgga ttagcccgcg
     3661 aatcggtcat tgctaccgct tgcggcattg cgtcgctcat gttgccgacg attcgcactt
     3721 cgcgttgcgc gaaaggaatc gagatgtcgt gttcggagaa caaacgccag atgttgcgat
     3781 taacggtcga gcgcacgctc gacgtgcccg tcgccgcgtc ctcgatccag aaacccagtt
     3841 ccagattgat accgtctgga ccaaagcttg ccagataagc ggtaggcgcc ggctcctgca
     3901 acacccgcgc gacgccctga gctgcctggg caagcaacgc catcgcctgc tcgacgtcgg
     3961 acgtgtaggc aatctgcacg gccaatttgg catagccgcg cgtcagatag gaagactggt
     4021 tctgcaccac gtccgtaatc agcttttcgt tcggaatcag cgtttcgatg ccatccaggc
     4081 cacgcaccac cgtatagcgt gtgcggatct gcgtgaccat accctgcaag ccgctgacat
     4141 tgatcgtgtc gccaatgcgc agcgagcgat cgatcagaat gatgaagccg gagacgtagt
     4201 tgctcgcgat cttctgcagc ccgaacccaa gcccgacacc cagcgcgccg ccgaacacgc
     4261 ccagcaccgt gatatcgatg cctaccagcg agaggctgat cagcaccgcc gccagcacga
     4321 acaccgcacg gccgacgcgc gccacgacca ctttgagatt ggcatcgagc gtggtcgagc
     4381 gaatcaggcg gtcttcgaac gccgagccca gccacatcgc gacaatcatc gtcacgcaga
     4441 cccacagaag acccgtgatc agcgacagca gcgtcatatg ggcattggcg acgcggaaat
     4501 gcacgctgcc catccacgcg atcacgtcgt cctgaatgcc catcacggtg agcaccatgc
     4561 cgatccacac gaccagcgag acgattttct cgacgaggaa tagccacgga tgggtctcgc
     4621 cgccctggct aaagacccgt cgagcgaaga aaaacacgat gtaaatcagg ccgataccga
     4681 acaagggcac cagcgccaga tcgagcagcg cggtgtgcat gaactggccg gtgatcgctc
     4741 gcgcaagcca gacgagtgcc gcgccgatca ggggaaagaa tgcacggttc aggctttccg
     4801 caccaaaacg cagggtttgg taacgcgtct gacgccgcag atcgagtgcc cggcgcaaca
     4861 gccgcgcgag cagccacgca atgatgagcg tgccaaccag cacgccgacc tgccacaaca
     4921 tcaccggctg gccgaagtcg cgcgcaaggt cgccgagcat gtgcgggaaa atgcggtttt
     4981 gcatgatgtc gctgaaatcg ccggcgcgcc gtgttccgga gggaacctgg ccgcgccggc
     5041 agatagcctg ttgcggttag ctctggcgtt ccagcacggc ggcgaagaag ccgtcagtgg
     5101 cgtggcggtg cggccacagc gacagataat cgcccatttc cagctcgatg cgctgttcgg
     5161 ccagcacgtc gcgcgcgggt accagggcga agtccggatg gtcggcgagg aattgctgca
     5221 cgacggcttc gttttccgct tccagaatcg agcaggttgc gtagacgagg cggccgccct
     5281 tcttcacgag gcgcgaggcg ctggcgagaa tcgacgcctg cttcggcgcc agttcggcga
     5341 ccgattcagg cgactggcgc cacttcaggt ccgggttacg gcgcagcgta cccaggccgc
     5401 tgcacggcgc atcgaccagc acgcggtcga tcttgccggc cagacgcttg atcttcgcgt
     5461 cgtgttcgct gtcgatcagc accggattca cgttcgacag gccgctgcgt gcaaggcgcg
     5521 gcttgagctt ggccaaacga cgctcggaga tgtcgaatgc atacagacgc cctgttgagc
     5581 gcattgccgc gcccagcgcc agcgtcttgc cgcccgcgcc cgcgcagaaa tcgacgatca
     5641 tctcgccgcg cttgggcgcg accagcgaac acagcaactg gctgccttcg tcctggactt
     5701 cgacccagcc gtgctggaat gcgtcaagct tggtgagcgg cggcttgccg accacccgca
     5761 cgccgaacgg cgcgaacggc gtcgcgccac cttcgatgcc agccttggag agcgcgttca
     5821 gcacgtcgtc gcggcttgcc ttgatcgggt tggcgcgcag gtccagcggc gccggatagt
     5881 tcagcgcggc ggccagttgc gccagttcct cagcctcgaa acgcctggag agcgcctgat
     5941 agatccagtc gggcagattc aggcgagtcc gcagcggcag gctttccggg tcgatcttcg
     6001 cgacatgttc gagccagtgc gactcggtgt ccgacacaaa cggcttgagg gccgtgcggc
     6061 cggccgtctg catcagaccc agcaacgcca tgcggcgggc cggactgccc gcgccgccct
     6121 cagccagatg ggcgaattcc atgcgacggc gcagcactgc gaagaccgcc tcggcgatca
     6181 cgccgcgctc gccgtggccg agcttcggat gcgcgcggaa aaagcggctc gtcgtggcgt
     6241 cggccgggcc gttcagtttc aggacttcag ccagcaaagt ctcggtctgt ccaatcaaaa
     6301 aaccatgtaa tctcatgcgc cctctccggc ggtgtgaccg gcaaagagcc attgcggctc
     6361 cggcggcgta agcgggacac gcaagccgtc gagagcaaga cgcccgtcga cgaaccagcg
     6421 caccgcgcgt ggataaataa tgtgttcggt tgccagcacg cgttcggcga gcatggcggg
     6481 ggtgtcgcca gcctcaacgg ggacggccgc ctgcgcgacg atcggcccgt gat
//
LOCUS       DATASH010000395         2020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3226684,
            whole genome shotgun sequence.
ACCESSION   DATASH010000395 DATASH010000000
VERSION     DATASH010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3226684"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /gene="clpX"
                     /locus_tag="VHV99_11065"
     CDS             complement(<1..869)
                     /gene="clpX"
                     /locus_tag="VHV99_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004521258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEX3636014.1"
                     /translation="MADKKGSNSEKLLYCSFCGKSQHEVKKLIAGPSVFICDECIDLC
                     NEIIRDEAAGAGIEAGLSKSDLPSPQEIREILDQYVIGQERAKKILAVAVYNHYKRLK
                     HLDKKDEIELSKSNILLIGPTGSGKTLLAQTLARLLNVPFVIADATTLTEAGYVGEDV
                     ENIIQKLLQNCNYEVDKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLVE
                     GTMASVPPQGGRKHPNQDFIQVDTTNILFICGGAFDGLEKVIVDRTEKTGIGFGASVK
                     SKQDRDAGEVLREV"
     gene            complement(1053..1706)
                     /gene="clpP"
                     /locus_tag="VHV99_11070"
     CDS             complement(1053..1706)
                     /gene="clpP"
                     /locus_tag="VHV99_11070"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006026149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEX3636015.1"
                     /translation="MTFRAQMLDTLTSQSSRDLEAQALGLVPIVVETSGRGERSYDIY
                     SRLLKERIVFLVGEVNDQTANLVVAQMLFLESENPDKDISFYINSPGGSVSAGMAIYD
                     TMQFIKPDVSTLCMGLAASMGAFLLAAGAKGKRFALPNSRVMIHQPLGGARGQASDIE
                     IQAREILYLKERLNHLLAHHTGQPVERIARDTDRDNFMSGDDAQAYGLVDQVAHKRP"
BASE COUNT          378 a          608 c          572 g          462 t
ORIGIN      
        1 acttcgcgca gcacttcgcc ggcgtcgcgg tcctgcttgc tcttcacgct tgcgccgaag
       61 ccgataccgg ttttctcggt acggtcgacg atgactttct cgaggccgtc gaatgcgcca
      121 ccgcaaatga acagaatatt ggtggtgtcg acctggatga agtcctgatt cggatgctta
      181 cgaccacctt gcggcggcac cgacgccatc gtgccttcaa ccagtttcag caaggcctgc
      241 tggacgccct cacctgatac atcacgggtg atcgacgggt tgtcggactt gcggctgatc
      301 ttgtcgattt cgtcgatgta gacaatgcct cgctgggcct tatccacttc gtaattgcag
      361 ttctgcagca gcttctgaat gatgttctcg acgtcttcgc cgacgtagcc ggcctccgtc
      421 agcgtggttg cgtcggcaat gacgaacggc acgttgagaa ggcgcgcaag cgtctgcgcg
      481 agcaaggtct tgccggaacc ggtcgggccg atcagcagga tgttgctttt ggacagctcg
      541 atctcatcct tcttgtcgag gtgcttaagg cgcttgtagt ggttgtacac cgcgaccgcg
      601 agaatcttct tcgcgcgttc ctgaccgatc acatactggt cgagaatttc acggatttcc
      661 tgcggactcg gcagatcgga cttggacaag cccgcctcga tgcccgcacc tgcagcctca
      721 tcgcggatga tttcgttgca caggtcgata cattcatcac agatgaacac cgacgggccg
      781 gcaatcagtt ttttgacttc atgctggctc ttgccgcaga acgagcaata caacagcttc
      841 tcgctgttag aacctttctt gtccgccata gatgtgtgag cctccgggca ctcgattaca
      901 tgatacgccg tttgccctgg cctcgcagtt tgggcgcggc agggcttgag ccacgaagac
      961 ggcgtttgcc gcaggcgctc ggacgagtct tgattatttc acaaccgatc cgacgacggc
     1021 ctcgcccctt tttggggcaa gaccgcacca gatcaaggac gcttgtgcgc cacctggtca
     1081 accagaccgt aagcttgcgc gtcgtcgccg gacatgaaat tgtcccggtc ggtgtcgcgt
     1141 gcgatacgtt cgactggttg gccggtgtgg tgtgcgagca gatgattcag ccgttccttc
     1201 aggtacagga tttcgcgcgc ctggatttcg atgtctgacg cctggccacg cgcgccgccc
     1261 agcggctgat gaatcatcac gcgcgaattc ggtaacgcaa agcgcttgcc cttcgcgccg
     1321 gctgccagca ggaacgcgcc catgctggcg gcgaggccca tgcacagcgt cgacacatcc
     1381 ggcttgatga actgcatcgt atcgtagatc gccatcccgg ccgaaaccga accgcccggg
     1441 ctattgatgt agaagctgat gtccttgtcc gggttttcac tttcaaggaa cagcatctgc
     1501 gcgaccacga ggttagccgt ctggtcgttc acttcgccga ccaggaacac gatacgttcc
     1561 ttcagcagac gcgaatagat gtcatacgag cgctcgcccc ggccgctcgt ttccacgacg
     1621 atcggcacca gtccgagcgc ctgcgcttcg agatcccgcg aagactggga ggtcaacgtg
     1681 tccagcattt gggcgcgaaa ggtcatgcaa tggatccttg tctggaaata ttctgaatat
     1741 tagctgctgc acacaggttg gtgcggcgag cccgtattca agtgccggtt tctgtgttcg
     1801 ccgcacgacc ttatccggta cgtgacacat tttaacggcg caaaaaaacg gcgtgcgggc
     1861 cgtcgctccg acagccggca cgccgttgca gcgacgctta cgcttgcgcc gttgcgcttg
     1921 ccagttcttc gaagctcact tccttgtccg tcaccttcgc tttgccgagg acgaagtcga
     1981 cgacgttggc ctcaacgacg tacgcttcca tttcagcaag
//
LOCUS       DATASH010000396         2428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3818615,
            whole genome shotgun sequence.
ACCESSION   DATASH010000396 DATASH010000000
VERSION     DATASH010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3818615"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="VHV99_11075"
     CDS             complement(<1..665)
                     /locus_tag="VHV99_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3636016.1"
                     /translation="MQTFDTVIRNARVVTAADIFTSDIGIRDGRIVALGLHLEAGARE
                     IDAAGRHVTPGGVDSHVHFDQPTGDGSIMADDFLSGTTSAACGGTTTVIPFACQQKGH
                     TLREAIDDYHRRAGRKPLIDYAFHLIVTDPTPEILRDELPALIAEGYTSFKIYMTYDA
                     LKLSDREMLDTLSVARSEGAMVMIHAENADCIAWLTERLLAAGHTAPRYHATSRPMLV
                     EREA"
     gene            complement(667..1392)
                     /locus_tag="VHV99_11080"
     CDS             complement(667..1392)
                     /locus_tag="VHV99_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp/Glu/hydantoin racemase"
                     /protein_id="HEX3636017.1"
                     /translation="MSRRIRLGILTPSSNTSLEPLTQALVAGLPEVSVHFARFPVTQI
                     ALSPDALGQFQRERILDAARLLADAHVDMIGWSGTSAGWLGFDQDEALCAAITETTGI
                     PASTSVLALNRALEAFGIRRLGLVSPYLADVQQKIVANYAALGIDASRESHLDLQENF
                     AFALVDEATLDRQVAEVVAGGAQAVTTFCTNLYAAHRVAHWEAQHGVPVFDTVTTVVW
                     DMLRRCGVDTRRITGWGRLVQEI"
     gene            complement(1389..>2428)
                     /locus_tag="VHV99_11085"
     CDS             complement(1389..>2428)
                     /locus_tag="VHV99_11085"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3636018.1"
                     /translation="EALRAGCTTLADSFVHAEATVEGIAQTGVRVFASQRLADVDFAS
                     VLAGERRFDRARGERQLERADAFVARWAGQANGRIQAHLTAHAPDTCSVALLEDIATL
                     ARRHDLPISTHLAQSTDEVDWVRQQHGRSPVELLDEIGLLDGRLLAGHCIHIDDADIA
                     RLGRSGAHVVHIPLGNAMSGRIAPTRRLLDAGARLCLATDTMHGDMIDVMRWALAMGR
                     LQAGAVDNDWQPSNVFAMATRHGAVALGWGARLGTLEVGRLADLAIFDFRQLHLTPSI
                     DPLGNLVHNATGSDVESVIVDGRLVIEQGRALCVDEHQIRADAQRAADAVWRRCPNPL
                     RHYPSVSTIRS"
BASE COUNT          415 a          764 c          843 g          406 t
ORIGIN      
        1 gcctcgcgtt ccaccagcat gggccgtgac gtcgcgtgat agcgtggcgc ggtatggccg
       61 gcggcgagca gccgctccgt cagccacgcg atgcagtctg cgttttccgc atggatcatc
      121 accatcgcgc cctcgctgcg cgccaccgag agcgtgtcga gcatttcgcg atcactcagc
      181 ttgagcgcgt cataggtcat gtagatcttg aacgacgtgt agccttcggc gatcaacgcg
      241 ggcaactcgt cgcgcagaat ctccggcgtc gggtcggtga cgatcaggtg gaacgcgtag
      301 tcgatcaagg gcttcctgcc cgcgcggcga tggtagtcgt cgattgcttc gcgcagcgtg
      361 tggcctttct gctggcacgc gaacggaatc acggtcgtgg tgccgccgca ggccgcggac
      421 gtggtgcccg agaggaagtc gtcggccatg atcgagccgt cgccggtggg ctgatcgaag
      481 tgaacgtggc tgtcgactcc accgggtgtc acgtgccgtc cagccgcgtc gatttcgcgc
      541 gcgccggctt cgagatgcag gccgagcgcg acgatgcggc catcgcgaat gccgatgtcg
      601 ctggtgaaga tgtccgcggc cgtgaccacg cgggcgttgc gaatgacggt atcgaaggtt
      661 tgcatgtcaa atttcctgaa cgagacggcc ccagccggtg atgcggcgtg tatcgacgcc
      721 gcagcggcgc agcatgtccc atacgacggt ggtgacggtg tcgaacaccg gcacgccgtg
      781 ttgcgcttcc cagtgcgcaa cgcggtgcgc ggcgtaaaga ttggtgcaga aagtcgtaac
      841 cgcttgcgcg ccgccggcca ccacctcggc cacttgacgg tcgagcgtcg cttcgtcgac
      901 cagcgcaaag gcgaagtttt cctgcagatc cagatggctt tcgcggctcg catcgatgcc
      961 gagcgcggcg tagttcgcga caatcttttg ctgcacgtcc gcgagatacg gcgagacgag
     1021 gccgagccgg cgaatgccga acgcctccag cgcgcgattg agcgcgagca cggacgtgct
     1081 ggcgggaatg cccgtcgtct ccgtgatcgc ggcgcacagc gcttcgtcct gatcgaagcc
     1141 aagccagccg gcggacgtgc cgctccagcc gatcatgtcg acatgcgcat cggcgagcag
     1201 gcgcgcggca tcgagaatgc gttcgcgctg gaactggccg agcgcgtcgg gactgagcgc
     1261 gatctgcgtg acgggaaaac gcgcgaaatg cacgctgact tcaggcagcc cggctaccag
     1321 cgcctgcgtc agcggttcga gagaagtatt ggatgagggc gtcaaaatgc ccagacggat
     1381 gcgtcggctc atgaacggat agtggaaaca gagggataat ggcgaagcgg attcgggcag
     1441 cgccgccaga cggcatcggc ggcgcgttga gcgtcggcgc ggatctggtg ttcgtcgaca
     1501 catagcgcgc ggccttgctc gatcacgagc cggccgtcga cgatgacact ttccacgtcc
     1561 gagccggtgg cattgtgcac gagattgccg agcggatcga tgctcggcgt gagatgcagt
     1621 tgacgaaaat cgaagattgc cagatcggcg agccggccga cctcgagtgt gccgagacgc
     1681 gcgccccaac cgagcgcgac ggcgccatgg cgcgtggcca tcgcgaacac gtttgacggt
     1741 tgccagtcgt tgtccaccgc gccggcttgc agacggccca tcgcgagcgc ccagcgcatc
     1801 acgtcgatca tgtcgccgtg catggtgtcg gtggcgaggc acaaccgcgc gccggcgtcg
     1861 agcaggcggc gcgtcggcgc gatacgccct gacatcgcgt tgccgagcgg aatgtgcacc
     1921 acgtgcgcgc cgcttcggcc cagacgcgcg atgtcggcgt cgtcgatgtg aatacagtgg
     1981 cctgcgagca gccggccgtc gagcaggccg atttcgtcga gcaattccac cggcgagcga
     2041 ccatgctgct gacggaccca gtcgacttca tccgtgcttt gcgcgaggtg cgtgctgatc
     2101 ggcagatcgt gacggcgggc tagcgtggcg atgtcttcga gcagcgcgac agaacaggtg
     2161 tccggcgcat gcgcggtgag gtgcgcctga atgcggccgt tcgcctgacc ggcccaacgc
     2221 gcgacgaacg cgtcggcacg ttccagctgg cgctcgccgc gggcgcggtc aaagcgccgt
     2281 tcgccagcca gcaccgaggc gaaatccacg tccgcgagcc gctggctggc gaacactcgc
     2341 acgccggtct gcgcgatacc ttcgacggtt gcctcggcat gcacgaagct gtccgcaagc
     2401 gtcgtgcagc cggcgcgcag cgcttcga
//
LOCUS       DATASH010000397         2066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1247682,
            whole genome shotgun sequence.
ACCESSION   DATASH010000397 DATASH010000000
VERSION     DATASH010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247682"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VHV99_11090"
     CDS             complement(<1..196)
                     /locus_tag="VHV99_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636019.1"
                     /translation="MNAVDEGRAIGEAGEVNAVDAVGKVGNVGMVDRSSSRGASSINH
                     NRKRSDAGSAARLARSVMTWV"
     gene            137..2066
                     /locus_tag="VHV99_11095"
                     /pseudo
     CDS             137..2066
                     /locus_tag="VHV99_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191952.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
BASE COUNT          310 a          671 c          717 g          368 t
ORIGIN      
        1 tcacccatgt catcaccgaa cgcgccagcc tggccgcgct gccggcgtcg gatcgtttgc
       61 ggttgtggtt gatcgacgag gcgccgcggc ttgacgatct gtcgaccatg ccgacgttgc
      121 cgaccttgcc gacagcgtcg accgcgttga cctcgccggc ctcgccgatc gcgcggccct
      181 cgtcgaccgc gttcacctgg cccaactcgc cgaccgcgtc gcccgcgccg acgatgttac
      241 ctaccgtctc gccacaccag gcggcctacg tgatctacac atcgggctcg accggcaagc
      301 cgaagggcgt cgtggtcgat cacgagtcgt tcgcgcggca ctgtgcggca attgccgaac
      361 gttacggcgc aggtgaaagc gacgtcttcc tgctgtttca gtcggtcaat ttcgacggcg
      421 cccacgaagg ctggttctcg cagtacatgt ccggcgccgc ggtggccatc accgccgaca
      481 cgttatggcc gcccgcgcaa acgtgccgga tgatggtgcg cgaaggcgtg acgatgacct
      541 acgtgccgcc gggttgcgcg acgcagcttg ccgaatgggc gctcgcccac ggcgcgccgc
      601 cgaccttgcg atcgctgacg gtgggcggtg aagcgacgtc gcgcgaagct ttcgcgctga
      661 tgcgtcgcgc gtttccagag gcgcgcatcg tcaacggtta tgggccgacc gagacggtca
      721 tcacgccgat gctgtggatg ttctatcccg ccgacgatgt cgcgaagctc gccgacagcg
      781 cttatctgcc gatcggcaca ttggtcggcg cgcgctccgc ccatgtgctc gacgctcgcc
      841 tgaatccgct gccggtcggt gtgatcggcg agctgtatct gggcggcgag ggcgtgggcg
      901 tcgcacgcgg ttatctcgac cagcccgcgc tcaccgccga tcgcttcgtg ccggatccgt
      961 tcggtgcacg aggcgcacgg ctctatcgca cgggcgacct cgtcaagcgg cgcgccgatg
     1021 gtgtgttcga ctttatcggc cgcgttgatc atcaggtgaa gctgcgcggc ttgcgtatcg
     1081 agctgggcga aatcgaggcg cagctcgccg cgcatgacga tgtgcgcgag gccgtggcga
     1141 tcgtgcacgg cacgggagcg caagcggccc tggtggcgta tgtcgagttg agcgtcgaag
     1201 cacgcgagcg ccacacgcgt gcggacgccg ccgaactcga cgcgcatttg cgtcatacgt
     1261 tgcccgatta catggtgccc gcgcatatcg tcgtgctcga tgcgttgccg cgcaacgcga
     1321 acagcaaggt ggaccgcgct gcgttgccgg agccgcagcg tgtcgcgcgg gtctatcagg
     1381 cgcccgcggc aggcctcgaa acggcgctcg ccgcgatttt ccgggaagtg ctgggcgtgg
     1441 agcgcatcgg tcgtggcgac catttcttcg aactcggcgg gcattcgctc gcggccgtgc
     1501 gggtggcgac gcgcgtagcc gagcggctcg gacgggatgt gcccgtgcgt tcgctcttcg
     1561 aagcgccgat cctcgctcag tacgcgcagc gcgtgatggc ggcggcgcgc agtgaggcac
     1621 tggtcgaagc ggatgtcgag tctgggttgc gggctgggtt cgcgagtgcg gcggcgaatc
     1681 cacgtgtgct tgccgatgcg caaggcgtgt tgcctttgtc cgcggcgcaa ttgagcctgt
     1741 ggttcttgtg gcgcacacag ccagacagcg cggcctacaa cattcctgtc gcgctgcgct
     1801 tgcatggacc gctcgatgtc gatgcattgc gtgaagcgtt tgccgtggcc gccgcgcggc
     1861 atccggcgtt gcgcacgcgg ctcgtcgaac gcgaagggga attgccggga caacggattg
     1921 ccgcgatagc gcctttcgaa ctgccggtga tcgaaatgtt tccgctcgaa gggagcggcg
     1981 cagcaaactc agcagacgca gcagacgcag cagacgcagc agacgcagca gacgcagccc
     2041 gcgaggctgc ggccgctacg ctgacc
//
LOCUS       DATASH010000398         2611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3026376,
            whole genome shotgun sequence.
ACCESSION   DATASH010000398 DATASH010000000
VERSION     DATASH010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3026376"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VHV99_11100"
     CDS             complement(<1..311)
                     /locus_tag="VHV99_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HEX3636020.1"
                     /translation="MNPTRTQLDAARWHWQNGPIDLILSADGEPSAVQAAYEACWMRF
                     VDVLPELVGELKLLRQPVPSSAAHGDCALKGPVARRMWDACHAHRARYITPMAAVAGS
                     VA"
     gene            complement(406..2013)
                     /locus_tag="VHV99_11105"
     CDS             complement(406..2013)
                     /locus_tag="VHV99_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxynicotinate reductase"
                     /protein_id="HEX3636021.1"
                     /translation="MTEHTKVDFGAESVDGETASTPGPTVLEKAAPRSERMASNKIEC
                     NACPVLCQISEGRTGACDRYANANGRLIRVDPVVFLSRAAALPGASLGKEDVTVEFGA
                     SSEVHDPAAEQALFVTGIGASSTYPDYKPAPFIVASKRDDVDMITVVTEGIFSYCSFK
                     VKIDTDRFLGPEQSNVRCRGEIVGHVTTAEYGSQMLSLGGVHHLTGGSKKEGRVTCDM
                     MLALGNKEAVELTIDGGASLVIRAGAAPIVDGVEEQRMRVGCGSATIGIFAKQWFGHA
                     DEVVVVDDHITGVLTEHQAGRCLGMTRSGLKIRGRKSTPGRYFQVANPGTGWGGTDIQ
                     DPLAIVEGFDPAAAKPGMRLLMVSTTGEHAQWYELDQDLVPRIAAMPDAVRRTVERIG
                     ENCEPSLATVLFLGGAGGSLRAGATENPVLLTRAIKQKLVNVTCGGAPAYVWPGGGIT
                     VMVDVSRMPDRSFGTVPTPAIVAPIEFTMTYDAYRDLGGHLDAVRSLESVLANGPEHT
                     EGAPLARRTLARNPDNPWPPAMPPMLG"
     gene            2407..>2611
                     /locus_tag="VHV99_11110"
     CDS             2407..>2611
                     /locus_tag="VHV99_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3636022.1"
                     /translation="MSSTAARKPGAIGIRARQAQDTRAKILKAAIKVFAKHGYASGRV
                     ESISKAARSHDRMIYYYFGSKEQL"
BASE COUNT          481 a          852 c          789 g          489 t
ORIGIN      
        1 gcgacgctgc cggcaacggc cgccatcggc gtgatgtagc gcgcgcggtg agcgtgacat
       61 gcgtcccaca tgcggcgggc tacggggcct ttcaacgcgc aatcgccatg agccgcactg
      121 ctcggcacag gttgccgaag cagtttcaac tcgccgacca actcaggcaa cacgtcgaca
      181 aagcgcatcc agcacgcctc gtaggcggcc tgcacggccg agggttcgcc gtccgcgctg
      241 aggatcagat cgatgggacc attctgccaa tgccagcggg ccgcgtcgag ttgggtgcgg
      301 gttgggttca ttgtgttcgg cgtgttcgtt gcgttcgctg cgttcggcgt gttcggcgtg
      361 ttcggcgtgt tcggcgcgtg ggtcgtgtcg gtcacgttgt cgccattagc cgagcatcgg
      421 cggcatggcg ggcggccacg ggttgtcggg attgcgcgcc agagtcctgc gtgcgagcgg
      481 cgccccttcc gtatgttccg gcccgttcgc caatacgctt tccagtgaac gcaccgcgtc
      541 gagatggccg ccgagatcgc gatacgcatc atatgtcatg gtgaattcga tcggcgcgac
      601 gatcgcgggc gtcggcacgg tgccgaacga tcgatcgggc atgcgcgaga catcgaccat
      661 caccgtgatg ccaccacccg gccacacata ggccggcgca ccgccgcatg tcacgttcac
      721 cagcttctgt ttgatggcgc gtgtgagcag caccggattc tccgttgccc ctgcccgcag
      781 actgccgccc gcgccgccga gaaacagcac cgtcgcaagc gacggctcgc aattctcgcc
      841 gattcgttcg accgtgcggc gcacggcatc cggcatggcc gcgatgcgtg gcacgaggtc
      901 ctgatcgagt tcataccact gggcgtgctc gccggtggtc gacaccatca gcaagcgcat
      961 gcccggcttc gccgcggcgg gatcgaagcc ttcgacaatc gccaacgggt cctgaatgtc
     1021 cgtgccgccc cagccggtgc cgggattcgc aacctgaaaa tagcggcctg gcgtcgattt
     1081 gcgtccgcga atcttcagac ccgagcgcgt catgcccaga caacgcccgg cctggtgttc
     1141 ggtgagcacg ccggtgatgt gatcgtcgac caccaccact tcgtcggcat gcccgaacca
     1201 ttgcttcgcg aagatgccga tggtggccga cccgcagccc acccgcatac gctgctcttc
     1261 gacgccgtcg acaatcggcg ccgcgcccgc gcgaatcacc agactcgcac cgccgtcgat
     1321 ggtcaactcc accgcctcct tgttgccgag ggcgagcatc atgtcgcacg tcacgcggcc
     1381 ttctttcttg ctgccgccgg tcagatgatg gacaccgcca aggctcagca tctgcgagcc
     1441 gtactccgcc gtggtcacat ggccgacgat ctcgccacgg caacgcacgt tcgattgctc
     1501 cggcccaaga aagcggtcgg tatcgatctt caccttgaag ctgcaatagc tgaaaatgcc
     1561 ctcggtgacg acagtgatca tgtcgacgtc gtcgcgcttc gaggcaacga tgaaaggtgc
     1621 gggcttatag tcgggatagg ttgacgatgc accaatgccg gtgacgaaca acgcctgttc
     1681 cgccgcggga tcgtgcactt cggacgatgc accaaattcg accgtgacat cctctttgcc
     1741 caggctcgca ccaggcaaag cagcagcgcg cgacagaaac accaccgggt cgacgcgaat
     1801 cagccgcccg ttcgcattgg cgtaacgatc gcacgcaccc gtgcggccct ccgatatctg
     1861 gcacaacacc gggcacgcat tgcattcgat cttgttcgac gccatccgct cgctgcgcgg
     1921 cgccgctttc tccagcacgg tgggaccggg cgtcgaggca gtttcgccgt cgacgctctc
     1981 cgcgccgaaa tcgaccttcg tatgttctgt catggcgggg ttcccatacg acgacattac
     2041 aatcagtctt gccctgctga cgaaaagccc ctgtttcagg ggactttcgc gtgatccgct
     2101 cgttttgatg ttgcggccac tcaccgttcg tgtagtatcc gctacacgaa acctgtttga
     2161 aatctacccg atcaaaatag actgcgcaac gagtttcccg gcgcgtgcct gaatgctgcg
     2221 gcgcggcatc ggcgcgcgaa tgacgcttca atctacgccg aagcgcatga tcgtgcaggg
     2281 cattccgtcg cgcgtgagat gggctgtatg atgaaggcgc ggcatgccaa agcgtacctg
     2341 cgtggtcgta agcgactgcg gcgcatgccg ttcaaccaga cgctaacaaa gagttccgag
     2401 accgccatga gttccactgc cgcccgcaag ccgggcgcta tcggcatccg agcgagacag
     2461 gcgcaagaca cgcgcgccaa gattctcaaa gcggcaatca aggtctttgc aaaacacggc
     2521 tatgcaagcg gccgggtcga aagtatttcg aaggccgcgc gttcgcacga ccgcatgatc
     2581 tattactact tcggcagcaa ggaacagttg t
//
LOCUS       DATASH010000399         8417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_852287,
            whole genome shotgun sequence.
ACCESSION   DATASH010000399 DATASH010000000
VERSION     DATASH010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 8417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8417
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852287"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VHV99_11115"
     CDS             <1..426
                     /locus_tag="VHV99_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEX3636023.1"
                     /translation="EEDTPLRRAKKLHGERLMTLMTTRSHDQYNTTIYALDDRYRGVF
                     GQRRVVFVNPSDLEMLGFKAGDWVDMTTVWDDGIERRADSFLLVEYDIPCGCIGSYYP
                     ETNPLVPLSSVGDVCNTPTSKSVPVLLHRSAPPAVSAAA"
     gene            503..1900
                     /locus_tag="VHV99_11120"
     CDS             503..1900
                     /locus_tag="VHV99_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007740758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEX3636024.1"
                     /translation="MPLEALHLARGQFAMTAIFHILWPILTISLSAFLLVVEILWVRT
                     SEVVWYRHARFWSKLLVLNFAVGVVTGIPMEFQFGTNWGPFSQFTGQFVGNILGFEGA
                     MAFMLEAGFVGIMMLGWGRVPKGVHLFATSMVALGSSISAFWIMVTNSWMQTPAGIAI
                     VDGKVVVTDYVKAIFNPDMVWGVSHMWFAALETGLVVVAGLSAYYLLKRRNPEFFVRS
                     FKLALLLLVFVAPVQIWLGDSSGVDVFATQPAKGAAIEGHWQTNAPGTGASWSLLAWP
                     DKAKQRNDWSIEVPGLLSVLGTHSLHGQVKGLADIPVADQPPLLPLLYYTFRAMAGIG
                     FLFMVLAFWTAFALRKARGRLDALLAQRKLLLAWVLAIPLPYVAVECGWIVREVGRQP
                     WVVYGLLRTEHAASAISPIAITGSMVMFAVFYVVLLSSFFYFARRWLKNGPDVTLAPP
                     AEPSQHSAPRTTVEY"
     gene            1946..2932
                     /gene="cydB"
                     /locus_tag="VHV99_11125"
     CDS             1946..2932
                     /gene="cydB"
                     /locus_tag="VHV99_11125"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007740757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEX3636025.1"
                     /translation="MNPVTAESLLSQAWFCLIGLMLVLYVITDGFDLGVGILSLFRSK
                     ESERDLMIQSIGHVWDANETWLVVLGGALFGAFPAAYATLLSDLYVPVMVLIASLIMR
                     GAAIEFRHAATGARPIWDRVFGIGSLLAGACQGVILGRVITGLLPGSLHLGFVAMTAI
                     GVVSGYALLGATWLVKKTDGRLQAAARRYSTISVVTTVAAAIVISIGTLVVSPIGAAR
                     WAQAGVFHVLIALAIVAALAFCHVLYSVYMGGEHGPFNASIVLFVASFAGLAVSLFPD
                     IVPGQLSIASAASNSTTLIFMLIGIGTVLPIMIGYNLYQYHLFRGKVIPVHH"
     gene            2969..3889
                     /locus_tag="VHV99_11130"
     CDS             2969..3889
                     /locus_tag="VHV99_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005254 - chloride channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family ion channel"
                     /protein_id="HEX3636026.1"
                     /translation="MIVRPPHNWFRMLFVWNGSVIQSIIPQLAVLALVGVLAVIAHDY
                     RFGASIPLNTTPFTLCGVALTIFLAFRNNASYDRFWEARRLWGNILISTRNLTSQALC
                     YVPDDAAKFDRTEFVRELIGFVHVLKHQLRNTDPTPDLLRCLGAERARVLHAKHYRAT
                     ALLNEVRHTVSRLHRRGAISDTQEWMFDNQLNELGTMVGGCERIASTPIPYPYGVLLH
                     RTVYAYCLLLPFGLVDSIGTATPLISVFVSYTLIALEAIASEVSEPFGLAPNNLALDA
                     MTRNIERSLLELCDEALPAEAKPDRRYQLT"
     gene            4045..4860
                     /gene="fdhD"
                     /locus_tag="VHV99_11135"
     CDS             4045..4860
                     /gene="fdhD"
                     /locus_tag="VHV99_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017332107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEX3636027.1"
                     /translation="MSCDLPTEHTGSVSRPIVRRRRGVSTVVTDRVVEECPVAIVFNG
                     ISHAVMMATPLDLEAFAVGFALTEGIVERAADIYDFEVFMHADSAQVQLTIAQPAFLQ
                     LKGKRRALAGRTGCGVCGIESIELLDLRPQKIARRKGAADVSPSAIESAARELPDRQI
                     LMRETGGIHAAAWCKPDGAIVEVFEDVGRHNALDKLIGGLALRQEDMQDGFVFLSSRA
                     SYELVRKTARMNVALLATISAPTSLAIDIAQRARMRLLSFCRQDGFVEYTSLG"
     gene            5261..5821
                     /locus_tag="VHV99_11140"
     CDS             5261..5821
                     /locus_tag="VHV99_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013689736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HEX3636028.1"
                     /translation="MSHEHAPHDEAIEHAARAGDPLSRWVAVFTAILAAIAGFLHYEE
                     GVLQTQALLFKNEAVLLQSKTSDEWNLYQAESSKGHMMEIAAGLSGPEKQGYYRDQIS
                     KYENRKKDIAAKAHEMEQQVAEANLQSGHKEHPQHMFGQALALINVAISLAAVTSLTG
                     SRKLFVVAACTGVVGIGVSIGAFLHI"
     gene            6210..7523
                     /locus_tag="VHV99_11145"
     CDS             6210..7523
                     /locus_tag="VHV99_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEX3636029.1"
                     /translation="MVSNGQVPLRLSDRTVSTINDVLAGRKAGGFRKLIFAGPAVVAS
                     VAYMDPGNFATNLQAGAQYGYSLLWVVVAANVIAMLFQALSARLGIVTNRNLAEMCRE
                     HFPMPVVYAMWGASEIAAMATDLAEFLGGAIGLSLLFHLPLIDGMVVTGVTIYAILLT
                     EKQGFRPIEVIIGGLVAAIATCYLVELFMVHVTWAAAARGTFVPTLHSSQAVTVAVGI
                     FGATVMPHAIYLHSGLTQNRAPARTEDARRKLVRFSNFEVIIALTLAGLVNMAMVVMA
                     SAAFHVGHPDIAEIETAYHTLTPLLGNGAAAVFLVSLLASGVSSSVVGTMAGQMIMQG
                     FVGFHIPVLLRRLVTMAPAFVVIALGVNATNALVCSQVVLSFALPMPMIALVIFTRRR
                     DIMGVFASSRLVSAAAILGTSVILVLNIILILQTFGVNLPGFHGA"
     gene            7775..7957
                     /gene="tatA"
                     /locus_tag="VHV99_11150"
     CDS             7775..7957
                     /gene="tatA"
                     /locus_tag="VHV99_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HEX3636030.1"
                     /translation="MGSLSIWHWLIVLVLVALVFGTKKLRTIGGDLGGAVKGFKQGIK
                     EAETATNAEHAELPRN"
     gene            8044..>8417
                     /gene="tatB"
                     /locus_tag="VHV99_11155"
     CDS             8044..>8417
                     /gene="tatB"
                     /locus_tag="VHV99_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HEX3636031.1"
                     /translation="MLDLGLTKMAVIGVVSLIVLGPERLPRVARTAGALLGRAQRYIS
                     DVKDEVTREIELSELQQVKSEFVAAAANIESAIHEDLREQAADLKVAWNDGYKASPDA
                     EEAAAQLPLQAPDAVPWRSGIAT"
BASE COUNT         1556 a         2537 c         2529 g         1795 t
ORIGIN      
        1 gaggaggata ccccgctccg ccgcgctaag aagctgcacg gcgaacgcct gatgacgctg
       61 atgacgaccc gttcgcacga ccagtacaac accacgatct acgcgcttga cgaccgctac
      121 cgcggcgtgt tcgggcaacg ccgtgtcgtg ttcgtgaacc ccagcgacct ggaaatgctc
      181 ggcttcaaag caggcgattg ggtcgacatg acgaccgtgt gggacgacgg catcgaacgg
      241 cgggccgact cgttcctgct cgtcgagtac gacattccgt gtggctgcat cgggtcgtac
      301 tacccggaga cgaacccgct cgtcccgctc tcgagcgtcg gcgacgtgtg caacacgccg
      361 acctcgaagt cggtcccggt gctgctgcat cggtccgcgc cgccggccgt aagcgccgcg
      421 gcgtgaggcc agcgcctcac gaattgtgca gcgcgccggg cggcgcgctc agctgatttt
      481 cactttgccc ttggtgcctg atatgccact cgaagcctta catctcgcgc gcggccagtt
      541 tgcaatgacc gctatcttcc atatcctgtg gccgattctg acgatcagtc tgtcggcctt
      601 tctgctcgtc gtcgaaattc tgtgggtacg cacgagtgaa gtcgtgtggt atcgccacgc
      661 gcgcttctgg agcaagctgc tcgttttgaa ttttgccgtc ggcgtcgtga ccggcattcc
      721 gatggagttt cagttcggca ccaactgggg gccgttttcg caattcaccg gccagttcgt
      781 cggcaacatc ctcggcttcg aaggcgcgat ggccttcatg ctcgaggcgg gcttcgtcgg
      841 catcatgatg ctcggctggg ggcgtgtgcc gaagggcgtg catctgttcg cgacctcgat
      901 ggtcgccctc ggatcatcga tctctgcgtt ctggatcatg gtcaccaact cgtggatgca
      961 aacacctgcc ggcatcgcaa tcgtcgacgg caaagtcgtc gtgaccgatt atgtcaaggc
     1021 catcttcaac ccggacatgg tctggggcgt gtcgcacatg tggttcgccg cgctcgaaac
     1081 cggcctcgta gtggtcgccg gtctttccgc ctactatctg ttgaagcggc gcaatcccga
     1141 gttcttcgtg cgttcgttca agctcgcgct gctgttgctc gtgttcgtcg ccccggtgca
     1201 gatctggctc ggggactcga gcggcgtcga cgtgttcgcc acgcagcccg ccaagggcgc
     1261 ggcaatcgaa ggccactggc aaaccaacgc tcccggcact ggcgcttcct ggtcgctgct
     1321 cgcatggccg gacaaggcga aacaacgcaa cgactggtcg atcgaagtcc ccggcctgct
     1381 gagcgtcctc ggcacgcaca gcctgcacgg ccaggtgaag ggcctcgcgg acattccggt
     1441 ggccgaccag ccgccactgc tgccgctcct ctactacacg ttccgcgcaa tggcgggcat
     1501 cggcttcctc ttcatggtcc ttgctttctg gacggcattc gcgttgcgca aggcgcgcgg
     1561 ccggctcgat gcgctactcg cgcaacgcaa gttgctgctc gcatgggtgc tggccattcc
     1621 gctgccctat gtcgcggtcg aatgcggctg gatcgtgcgc gaagtcggtc gccagccatg
     1681 ggttgtgtac ggcttgctcc gcaccgaaca cgcggcctcc gccatctctc ccattgcgat
     1741 taccggcagc atggtgatgt ttgccgtctt ctatgtggtc ttgctgtcgt cgttcttcta
     1801 cttcgcacgc cgctggctga agaacggccc cgacgtcacg ctcgcacccc cggccgagcc
     1861 gtcacagcat tccgccccgc gcaccaccgt cgagtattga tccggccacc gctccgttca
     1921 agataatcga aacaggtcac acaaaatgaa tccagtcacc gctgaaagtt tgttgagtca
     1981 ggcatggttt tgcctgatcg ggctgatgct cgtgctttac gtgatcaccg acggcttcga
     2041 cctcggtgtg ggcatcctga gtctcttccg ttcgaaggaa tccgaacgag acctgatgat
     2101 ccagtcaatc gggcacgtct gggacgccaa cgaaacgtgg ctcgtcgtgc tcggcggcgc
     2161 cctcttcggg gcattcccgg cagcgtatgc gactttgctg tcggacctgt acgtgcctgt
     2221 gatggtcctg atcgcgagcc tgatcatgcg tggcgccgcg atcgaattcc gtcacgccgc
     2281 aacaggcgca cggccgatat gggaccgcgt gttcggcatc ggcagcctgt tagcgggggc
     2341 gtgccagggg gtgattctcg gcagagtcat caccggcctg ttgccgggca gccttcacct
     2401 gggattcgtc gcgatgaccg caatcggcgt cgtgtcgggc tatgcgctgc tcggtgcaac
     2461 gtggctcgtg aagaagaccg atggcaggct gcaagcggcc gcacggcgct actcgacgat
     2521 ctcggtcgtg acgaccgtcg ccgccgccat cgtgatctcg atcggcacgc tggtagtcag
     2581 cccgatcggc gccgcgcgct gggcgcaagc cggtgtgttc cacgtgctga tcgctctcgc
     2641 gatcgtcgcg gcgctggcgt tctgccacgt cctctattcg gtgtatatgg gcggcgagca
     2701 cgggccgttc aatgcgtcga tagtcctgtt cgtcgcatcg ttcgccggcc tcgctgtcag
     2761 cctcttcccg gatatcgtgc cgggacagtt gagcatcgcg tctgccgcgt cgaacagcac
     2821 gacgctgatc ttcatgctga tcggcatcgg caccgtgctg ccgatcatga tcggctacaa
     2881 cctgtaccag taccacctgt tccgcggcaa ggtcattccg gtccaccatt gatcgatacc
     2941 tttacaccgc caaacactgg agtgcaaagt gatcgtcagg cccccgcata actggttccg
     3001 catgctgttc gtgtggaacg gatcggtaat tcaatcgatc attccgcaac tggccgtcct
     3061 cgcgctggtg ggggtactgg cggtcatcgc ccacgactac aggttcggtg cgagtattcc
     3121 gctcaacact acgccgttca cgctgtgcgg tgtggcgctc acgatcttcc tcgcatttcg
     3181 gaacaacgcg agctatgacc gtttctggga ggcccggcgc ctgtggggga atatcctcat
     3241 ctcgacgcgc aatctgacgt cgcaagcgct ttgctacgtg ccggacgatg cggctaagtt
     3301 cgaccggacc gagttcgtgc gcgagctcat cggcttcgtc catgtactca agcatcaatt
     3361 gcgcaacacc gatccgacgc ccgatctcct gcgctgcctc ggcgccgagc gagcccgtgt
     3421 gctgcatgca aaacactatc gggccaccgc gctcctgaat gaggtccggc atacggtgag
     3481 caggttgcac cggcgcgggg cgatctccga cacgcaggag tggatgttcg acaaccagct
     3541 aaacgaactg ggcacgatgg tcggcggctg cgagcgaatc gcgtcgacgc cgatccccta
     3601 cccctacggc gtgctgcttc atcgcaccgt gtacgcctac tgcttgctgc tgccgtttgg
     3661 tctggtcgat tcgatcggca ccgccacccc gctcatttcc gtgttcgtgt cgtacacgct
     3721 gattgcgctc gaggcgatcg caagcgaagt atccgaaccc ttcggccttg cgccgaacaa
     3781 cctggcgctc gacgcgatga cgcgcaatat cgagcgctcg ctgctcgaac tgtgcgacga
     3841 agcgcttcct gccgaagcga aaccggaccg gcgctaccaa ctgacgtaag tacggcctgc
     3901 atcgcgcggc tgccattctg tagcttgccg atgcgatcca cttcgtaccc tccggccgtt
     3961 cgccgcctgc gtacgattct ccatacggaa tgcgggccgg ctcgagcgcg acgccccgca
     4021 ctccactgcc ccgaaggaga aaagatgtcc tgcgatctac ccaccgaaca tacaggaagc
     4081 gtttcgcgcc cgatcgttcg ccgccggcga ggcgtgtcga cggttgtcac ggatcgagtc
     4141 gtcgaggaat gcccggttgc gattgtcttc aacggcattt cgcacgctgt catgatggcg
     4201 accccgctcg atctcgaagc attcgcagtg ggctttgcgt tgaccgaagg gatcgtcgag
     4261 cgcgccgccg acatctacga tttcgaagtc ttcatgcacg ccgacagcgc gcaggtgcag
     4321 ctgacgattg cgcaaccggc gttcctgcaa ttgaaaggca agcggcgcgc cctcgccggc
     4381 cgtaccggct gcggcgtgtg cggaatagaa agcatcgaat tgctggatct gcgaccgcag
     4441 aagatcgcgc gtcgaaaggg tgccgccgac gtatcgcctt cggcgatcga atcagccgcg
     4501 cgcgaactac cggaccggca gatcctcatg cgcgagacag gaggaatcca cgctgcggca
     4561 tggtgcaagc ccgacggcgc catcgtcgaa gtgttcgagg acgtgggtcg ccacaacgcg
     4621 ctcgacaagc tgatcggcgg tctcgctctc cgccaggaag acatgcagga cggcttcgtc
     4681 tttctgtcga gccgggcgag ctacgagctg gtgcgcaaga cggcacgaat gaacgttgcg
     4741 ttgctcgcca cgatatcggc gccgacctcg ctcgcgatcg acatcgctca acgtgcccgc
     4801 atgcgtctct tgagtttctg ccggcaagac ggctttgtcg agtacacaag cctcggctga
     4861 agccgaactt ccgacatggc cgacgagggg acgggtcaac gtgccgaatg aagggctgcg
     4921 tcgtcggccg atgttcgaat gcgtgtccaa ttgctcgagc gaacggccca gcccggccgt
     4981 gaattgcccg acgcatcgca cccacttcgc cccctgaagt gacgttcgtc agtgtcgcgt
     5041 cgaccggcag aggatcggcc ataccttggc gttggactcg acaccggtcg atggccaacc
     5101 ggggcaacgg gcaacaggtc aggtgagacg ttgcgccgca gtgcgtacgg ggcggccagg
     5161 atctatgctt atcccgtctc ctccatgtac ttcctcagca tatggattcg gcccgccagc
     5221 tcaggcgggc ctttttttat ttcaaaacgg agagtaaaac gtgtcgcacg aacatgcccc
     5281 ccatgacgaa gccatcgaac acgcagcgcg tgcaggcgat cctctatcgc gttgggtagc
     5341 cgttttcacc gcgatccttg ccgccatcgc gggtttttta cattatgaag aaggtgtgct
     5401 gcagacgcag gccttattgt tcaagaacga ggccgtgctg ctgcaaagca agaccagcga
     5461 cgaatggaat ctttaccagg ccgaatcaag taaaggccac atgatggaga tcgctgccgg
     5521 gttatccggg cccgaaaagc agggctacta tcgcgatcag atcagcaagt acgagaaccg
     5581 aaagaaagat atcgcagcga aggcgcatga gatggaacag caggtagccg aagcaaacct
     5641 gcaatcgggg cacaaggagc atccgcagca catgtttggt caggcgctcg cgctaatcaa
     5701 tgtggctatt tcgctagcgg cggttacgtc gttgacgggc agtcgaaagc tcttcgtcgt
     5761 ggcggcctgc acaggggttg tgggcatcgg cgtgtctatc ggtgcctttt tgcacatatg
     5821 agtcggcttt ccaggtctgt cgccccgaaa gagacagggc ggggtgagga gaaacgcccc
     5881 gctcggatcg cgaatcgacg atctgctcca tcagtcgcac aattaacatt ttttcatatt
     5941 cgcgccattc ccgttcagtt ggctcgcaca tatcctcacc tttagctgaa tccatgcgcg
     6001 gtcgtcgcgt ttcgaaccaa ccaggccgtg aaggtcggtt gagcatccga cgcccactac
     6061 tgaagttgag ttagaggcat ttttgattgt ctgaacggca acctcgcctc gcgaaaatct
     6121 gccagacatg caccggatgt ggcctgcagt ctctttgctt agccttgctc tggcgtcgag
     6181 cggtttagcc cattttccaa gaggattcga tagtgagtaa tggacaagtc ccgctaaggt
     6241 tgtcggatcg gacggtaagc acaatcaatg atgtgctggc tggcagaaag gctggtggtt
     6301 tcagaaaatt gatctttgcc ggtccagctg tcgtggcgtc agtcgcctac atggatcccg
     6361 gcaacttcgc cacgaacctt caggcgggcg cccagtacgg ctattccttg ctctgggttg
     6421 tcgtggcagc gaacgtcatt gccatgctgt tccaggcgct gtctgccaga ctcggtatcg
     6481 tgaccaaccg gaatctcgct gagatgtgcc gcgagcattt cccgatgcca gtggtttatg
     6541 caatgtgggg cgccagtgaa attgccgcga tggcaacgga cctggcggag ttcctaggcg
     6601 gggcaatcgg tttgtcgctc ctgtttcacc tgccgttgat cgacggcatg gtggtaacag
     6661 gggtgaccat ctacgcgata ctactgacgg aaaaacaggg tttccgacca atcgaggtga
     6721 tcatcggagg gctcgttgcg gcaattgcca cgtgctatct cgttgaactg ttcatggttc
     6781 atgtgacgtg ggccgcagcc gcccgcggaa ccttcgtgcc aacgttgcac tcgagccagg
     6841 ctgtcacagt cgcagtcgga attttcggcg ccaccgtgat gccgcacgcc atctatctgc
     6901 actctggcct gacccagaac cgcgcgcctg cccgtaccga ggatgcgcgc cgtaagctgg
     6961 tgcgtttctc caattttgag gtgattattg cgctcacgct tgcaggcctc gtcaatatgg
     7021 cgatggtcgt gatggcgtcc gcggcatttc acgttgggca ccccgacata gcagaaattg
     7081 aaacggctta ccacacgctg acccctttgc tcggcaacgg tgctgcagct gttttcctcg
     7141 tttcgctgct tgcctccggc gtttccagct cggtcgtggg aacgatggcg gggcaaatga
     7201 tcatgcaggg ttttgtcggc tttcatattc ctgtgttgct tcgccgccta gtgacgatgg
     7261 cgcccgcatt tgtggtgatc gcgttgggcg taaatgccac caatgcactg gtctgtagtc
     7321 aggtggtctt gagttttgcg ctccccatgc cgatgattgc gctggtgatc tttacccgca
     7381 ggcgcgacat catgggggtg tttgcctcca gccggctggt tagcgcggcg gcgattcttg
     7441 gaacgtcggt aattcttgtg ctcaacatca ttctgattct gcaaacattt ggcgtgaacc
     7501 tccccggctt tcatggggcc tgacaggcta tgtcaaacag aaagtacgca atacgtaaca
     7561 tgtagtatgc agggcaatgc gacaacgctt cttgctgctg cagcggcggc gggcatggct
     7621 ctttctcttg cccggaacga tctaaaaagc actgggctcg gcgccctcaa cattcgctgt
     7681 tccattgaag cgcacaaacg cttcgcgttc gaccaactgg tcgaagcaaa ttctcgccgc
     7741 gcaaatcaat gcgacgggat caaggagagt tttcatgggt tcactaagta tctggcattg
     7801 gctgattgtt ctggtgcttg tcgcgctggt tttcggcacg aaaaaacttc ggaccattgg
     7861 cggcgatttg ggcggcgcgg taaaaggctt caagcaagga ataaaggaag ccgagacagc
     7921 cactaatgct gagcacgcag agttgccgcg caactagaca gttgaaggtc gagaccaaaa
     7981 aaacctttgg gtccggcgaa attcgttgaa cgacaatcag tcatgtgctg acggaacacc
     8041 tccatgcttg atctaggtct taccaagatg gcagtcatcg gtgtggtctc gctgatcgta
     8101 ctggggccgg agcgactacc acgcgtagca cgcacggccg gagcgctcct tggccgcgcg
     8161 caacgctata tcagcgacgt gaaagacgaa gtcacgcgcg agatcgaact cagtgaactg
     8221 cagcaagtga agagcgagtt tgtggcggct gcggcgaaca tcgagagtgc cattcacgaa
     8281 gatctgcgtg agcaggcggc ggatctcaag gttgcgtgga atgacggtta taaggcgtcc
     8341 ccggatgccg aggaagctgc ggcgcagctt ccgttgcaag cgccggatgc cgttccgtgg
     8401 cgcagtggta ttgccac
//
LOCUS       DATASH010000400         1504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4413849,
            whole genome shotgun sequence.
ACCESSION   DATASH010000400 DATASH010000000
VERSION     DATASH010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4413849"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1425)
                     /locus_tag="VHV99_11160"
     CDS             complement(<1..1425)
                     /locus_tag="VHV99_11160"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEX3636032.1"
                     /translation="MAKRYGGVVALDNLSIELNAGEVLGLLGANGSGKSTLSSIIAGD
                     NRADEGVIRLNGVELPTGSPGAARAAGIAIAHQHPGLAPDLPVWENVYLGAEICGAGS
                     FLKRATARRKAGELLSSLRAGWDLDAPAGTLTAADQQLVEIAKALALEPRVLIFDEPT
                     AALAAAEVESLMLAIRTLAARGTAIVFISHRMAEVEALCDRLVVLCNGRHAGELAVNG
                     QIDEARVLAWMGGAAGGAAAAKLHEAGTGESAQTPPQRLQMAYTDQPQVALNARGLRA
                     GTRLHGVDFDVNRGEILGIAGLQGHGQEELLDALAGYRKLASGEITLDGAPLAPRSPR
                     QMIEAGVCLVPNDRHRQGLWLDHSVEFNLAQVGVNFERRAWRLERAPIKRFVEDVVAR
                     LRIKTSNVRQPVRALSGGNQQKVVIGRWLDARLKLKVLLLSDPTKGVDVIARKEIYEA
                     VGALAGTGAAVIVYASDTDELLAVC"
BASE COUNT          271 a          500 c          455 g          278 t
ORIGIN      
        1 gcatacggcc agtagttcgt ccgtatccga tgcgtagacg atcaccgctg cgccggtccc
       61 agcgagcgca cccacggctt cataaatctc cttgcgggcg atgacatcga ctcctttagt
      121 cggatcgctg agcaacaaca ctttgagctt caggcgcgcg tcgagccagc ggccgatcac
      181 gactttctgc tggttgccgc ccgacagcgc gcgcaccggc tggcggacat tggacgtctt
      241 gatgcgaagg cgtgcgacca cgtcttcgac gaagcgcttt atgggcgcac gttccagccg
      301 ccacgcgcgc cgttcgaaat tcacgccaac ttgcgcgaga ttgaattcga cggagtgatc
      361 gagccagagg ccctggcgat gacgatcgtt aggcacgaga cacacgcccg cttcaatcat
      421 ctgccgcggt gagcgtggcg caagtggcgc accgtcaagc gtaatctcac cgctcgcgag
      481 cttccgatac ccggcgagcg catcgagcag ttcttcctgg ccgtgcccct gcaaccctgc
      541 gataccgagt atctcgccgc ggttgacatc gaaatcaacg ccgtgcagac gggtgcccgc
      601 ccgcaggccg cgcgcgttca gcgcgacttg gggctggtcg gtgtatgcca tttgcaaccg
      661 ttgaggagga gtttgtgcgg actcacctgt acctgcctcg tgcagctttg ccgcagcggc
      721 gccgccggcc gcgccgccca tccacgccag cacgcgcgct tcatcgatct gaccgttcac
      781 ggccagttcg cctgcgtgac gtccgttgca caagacgacc agacggtcac acaacgcttc
      841 gacttcagcc atgcgatggg aaataaagac aatagccgtg ccgcgcgcgg ccagtgtgcg
      901 gatcgcgagc atcaggctct cgacttcggc agcggccagc gcggctgtcg gttcgtcgaa
      961 gatcagcaca cgcggctcca acgcgagcgc ttttgcgatc tccaccaatt gctgatcagc
     1021 ggccgtcagc gtgccagcag gcgcatcgag gtcccagccg gcgcgcagcg agctcagcaa
     1081 ttcgcccgcc ttcctgcgtg cagtcgcgcg cttcaggaaa cttcccgcgc cgcaaatctc
     1141 ggcgccgagg tacacgttct cccataccgg caagtcgggc gcgagaccgg gatgctgatg
     1201 cgcgatcgca atgcctgcag cgcgtgccgc gcccggagaa ccggtgggaa gttcgacgcc
     1261 attcaggcgg attacgcctt cgtctgcacg gttgtcgccc gcgatgatgc tggagagcgt
     1321 cgacttgccc gagccgttcg cgccgagcaa tcccagcact tctcccgcgt tcagctcgat
     1381 gctgagattg tcgagtgcaa ccacgccgcc gtaacgtttc gccatcccgt gcgctgtgag
     1441 aagtgggcta gcctgcatcg tgaatccgct tgaatttact gcgtgaaaag ggcatcgagt
     1501 tcag
//
LOCUS       DATASH010000401         4579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5798020,
            whole genome shotgun sequence.
ACCESSION   DATASH010000401 DATASH010000000
VERSION     DATASH010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4579
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5798020"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3511
                     /gene="cobN"
                     /locus_tag="VHV99_11165"
     CDS             <1..3511
                     /gene="cobN"
                     /locus_tag="VHV99_11165"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HEX3636033.1"
                     /translation="IEQVVALAERNQQTLAMFSGDLQEDPNLLARSTADAELCHQLWR
                     YLREGGPQNAEAFLRFIAYRSFGWGREPAPPRALPAATLYHPERDTPTISDWQARWRE
                     DAPVAAILFYKAHLQAANTAVFDALIEALEAQGLNALPIAITSLKDAMSRDVVQQLCA
                     QHDVALVLNTTAFAASAIDDPEPLALAGDAPVMQVILSGGNREDWVKDNHGLNSRDIA
                     MHIALPEVDGRIITRAISFKGLAYRCPRTQVDVVRYQPDRERVGFLAELSRRWCRLHT
                     LGNADKKLALILANYPMSEGRIGNGVGLDTPASVVGILSMLRDEGYRLAELPADGDTL
                     LKKLTEGVTNDPVVRDLRPALQSLALDDYLVYFNALPVEARDALNARWGSPEQDPTVR
                     RGRFMIAGWRCGHAFVGIQPSRSRQQGDYASYHDAELVPPHAYLAFYFWLRHQFCIDA
                     IVHVGKHGNLEWLPGKSVALSDACWPDLILGPMPHLYPFIVNDPGEGSQAKRRTQAVI
                     IDHLMPPLTRAESYGPLQDLERQVDEYYEALMVDPRRSKLLRSTILATIVEHKLHEEL
                     SLAEPTGQDDEDVLLTRVDAWLCELKEAQIRDGLHTFGQSPEGVQRRDTLLALGRFPV
                     GDGHGAKAGLIDALARDLQMDHLFDPLSVDWAAVWDGPRPQVLQRVSDAPWRHCGDAR
                     ERLERLAAEMLRGVCGDETSDAMPASGTLPQAERVIERLRNDVLPRLDACGPQEMLHL
                     KRGLEGRFVPPGPSGSPSRGRPDVLPTGRNFYSVDTRAVPTQAAWSLGLKSAQQLIER
                     HLQEKGDYPRAIGLSVWGTATMRTGGDDIAQALALLGVRPKWAPGSHRVTDFEIMPIE
                     AFDRPRIDVTLRVSGFFRDAFANVMHLFDAAVQAVAELDEPEDLNPIRARVLRERDAL
                     IARGMDATEARKRAGWRVFSARPGAYGAGLQDMIDSQQWDTDADLANAYQAWGGYAYT
                     QKSAGEEARHAFGTRLAAMDVVLQNQDNREHDLLDSNDYYQFQGGMVAAVRHLAGNQP
                     QVYHADHSNPAAPRVRTLHEEIARVIRSRVVNPKWIDGVKRHGYKGAAEIAATVDYLY
                     GYDATTRVIADHQYALVTDAYLNDADTREFLQRHNPHAMHSICERLLEAMQRGLWQAP
                     GDYRAQIEQHLLASEQQIEGMQT"
     gene            3508..4572
                     /locus_tag="VHV99_11170"
     CDS             3508..4572
                     /locus_tag="VHV99_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3636034.1"
                     /translation="MNAATQRPVFPFAALIGQTPLQQALLLAAIDPGLGGVLVSGPRG
                     TAKSTAARALAELLPEGQWVNLPLGASEDRLIGTLDIETVLRDGSVRFSPGLLAKAHR
                     GVLYVDEVNLLPDALVDALLDAAASGVNTVERDGVSHSHDASFVLIGTMNPEEGELRP
                     QLIDRFGLMVELQNCFEPRVRQQIVKARLAFDLDPQGFRAGYVQQQETYVQRIRAARE
                     ALPQLDFDDDVHAHVSALCIDAAVDGLRADLVMLRAARALAALEQAAVVTTGHVERVA
                     EAALMHRRHDRETRADGDARESEAPRQTTGDADATAWRASQDAAPGAAAGDSDWGYLP
                     PQPAGVTQVKGVIALNAKKR"
BASE COUNT          825 a         1363 c         1526 g          865 t
ORIGIN      
        1 catcgaacag gtggtcgcgc ttgccgaacg taaccagcag acactcgcga tgttctccgg
       61 cgatttgcag gaagatccga acctgctcgc acgcagcacc gccgacgccg aactgtgtca
      121 tcagctatgg cgctacctgc gtgaaggcgg cccgcagaat gccgaggcgt tcctgcggtt
      181 tatcgcatac cgctcgtttg gttgggggcg cgaacctgcg ccgccgcgcg cgctgcctgc
      241 cgcgacgctc taccatcccg aacgcgatac gccgacgatt tccgactggc aggcgcgctg
      301 gcgtgaggat gcgcccgttg ctgcgattct gttctacaag gcgcatctgc aagcagccaa
      361 tacagcagtg ttcgacgcgc tgatcgaggc gctcgaagca caaggcttaa atgcgctgcc
      421 gatcgcgatt acctcgctga aagatgcgat gagccgcgac gtcgtgcagc agttgtgcgc
      481 gcaacatgat gtcgccctgg ttttgaacac gacggcattc gccgcctcgg ccatcgacga
      541 ccccgagccg cttgcgctcg ccggcgacgc gccggtgatg caggtaatcc tgagcggcgg
      601 caatcgcgaa gattgggtca aggacaatca cggcctcaat tcgcgcgata tcgccatgca
      661 tatcgcgctg ccggaagtgg acggccgcat catcacgcgg gcgatcagct tcaagggcct
      721 cgcctatcgt tgtccgcgca cccaggtcga tgtagtccgc tatcaaccgg accgcgagcg
      781 cgtcggcttt ctggccgaac tgagccgccg ctggtgccgt ctgcacacgc tcggcaacgc
      841 ggacaagaag ctcgcgctga ttctcgccaa ctatccgatg agcgaagggc gcatcggcaa
      901 tggtgtgggg ctcgatacgc cggcctcggt cgtcggcatt ctgtcgatgt tgcgcgatga
      961 gggatatcgt ctcgccgagt tgcccgccga cggcgacacg ctgttgaaga agctgaccga
     1021 aggcgtgacc aacgatccgg tcgtgcgcga cttgcggccc gcgttgcaaa gcctcgcgct
     1081 cgacgactat ctcgtgtact tcaacgcatt gcccgtggaa gcgcgcgatg cgctgaacgc
     1141 acgctggggt tcccccgaac aggatccgac ggtgcggcgc ggccgtttca tgattgcggg
     1201 ctggcgctgt gggcatgcgt tcgtcgggat tcagccgtcg cgttcacggc agcagggcga
     1261 ttatgcgagc tatcacgatg ccgaactcgt gccgccgcat gcgtatctgg cgttctactt
     1321 ctggctgcgt catcagttct gtattgacgc gatcgtgcat gtcggcaagc acggcaatct
     1381 ggaatggctg ccgggcaaga gcgtcgcgct gagcgatgcc tgctggcccg atctgattct
     1441 tgggccgatg ccgcatctgt atccgttcat cgtcaacgat ccgggtgaag gcagtcaggc
     1501 gaagcgtcga acgcaggccg tcatcatcga tcatctgatg ccgccgctca cgcgcgccga
     1561 gagctatggg ccattgcagg acctcgaacg ccaggtcgac gaatactatg aagcgctgat
     1621 ggtcgatccg cgccgttcga agctgttgag gagcacaatt ctcgcgacca tcgtcgagca
     1681 taagctgcat gaagaactga gtctcgcgga accgaccggt caggacgatg aggacgtgct
     1741 gctgacgcgc gtcgatgcct ggctgtgtga attgaaggaa gcgcagattc gcgatggttt
     1801 gcacacgttc gggcaatcgc ccgagggtgt gcagcgacgc gatacgttgc ttgcgctggg
     1861 ccgttttccg gttggcgatg gtcacggcgc caaggccggt ttgatcgatg cattggcgcg
     1921 cgatctgcag atggatcatc tgttcgatcc gctgtcggtg gattgggccg ctgtttggga
     1981 cggtcctcgt ccgcaggtat tgcagcgtgt gagcgatgcg ccctggcgac actgtggcga
     2041 tgcacgcgag cgtctggaac ggttggcggc cgagatgttg cgcggtgtgt gtggtgatga
     2101 aacgtccgat gcgatgcccg cgagcgggac cttgccgcag gccgaacgcg tgatcgaacg
     2161 cctgcgcaac gacgtgctgc cgcggctcga cgcctgcggt ccgcaggaaa tgctgcatct
     2221 gaagcgcggt ctcgaagggc gtttcgtgcc gccggggccg agcggttcgc cgtcgcgtgg
     2281 acgtcccgac gtgctgccca ccggccgcaa cttctattcg gtcgatacgc gcgcggttcc
     2341 gactcaagcg gcgtggtcgc tcgggttgaa atccgcgcag cagttgatcg aacggcactt
     2401 gcaggaaaaa ggcgactacc cgcgtgcgat tggcctctcg gtatggggca cggcgaccat
     2461 gcgcaccggc ggcgacgaca tcgcccaggc actcgctcta ctcggcgtgc ggccgaagtg
     2521 ggcgccgggc agtcaccgcg tgaccgactt cgagatcatg ccgatcgagg cattcgaccg
     2581 gccacgcatc gacgtcacct tgcgcgtgtc cggctttttc cgcgatgcct tcgccaacgt
     2641 gatgcatttg ttcgatgccg ccgtgcaggc cgtggccgaa ctcgacgagc ccgaggacct
     2701 gaacccgatc cgcgcgcgcg tgctgcgcga acgcgacgcg ctgatagcac gcggcatgga
     2761 cgccaccgaa gcgcgcaagc gcgccggctg gcgcgtgttc agcgcacggc ccggcgcgta
     2821 tggcgcgggt ctgcaggaca tgatcgactc gcagcaatgg gacaccgacg ccgatctcgc
     2881 gaatgcctat caggcgtggg gtggttacgc ctacacgcag aaaagtgccg gtgaagaagc
     2941 gcgtcacgcc ttcggcacgc gtctcgccgc gatggatgtg gtactgcaga accaggacaa
     3001 ccgcgagcac gacctgctcg attcgaacga ttactatcag tttcaggggg ggatggtcgc
     3061 ggcggtgcgg catctggcgg gcaatcagcc gcaggtctat cacgcggacc acagtaatcc
     3121 ggcggcaccg cgcgtgcgca cattgcatga agaaatcgcg cgtgtgatcc gctcgcgcgt
     3181 ggtcaatccg aaatggatcg atggtgtgaa gcgacacggc tataaaggcg ccgctgaaat
     3241 cgccgcgacg gtcgactatc tgtacggcta cgacgcgacc acgcgcgtga tcgccgatca
     3301 tcagtacgcg ctcgtcaccg atgcttatct gaacgacgcc gatacacgtg aattcttgca
     3361 acggcacaat ccacacgcca tgcattcgat ttgcgagcgt ctgctcgagg cgatgcagcg
     3421 cggcttgtgg caagcgcccg gcgactatcg cgcgcaaatc gagcagcatc tgctcgcgag
     3481 tgaacagcag atcgaaggaa tgcaaacatg aacgctgcga ctcaacggcc cgtttttccc
     3541 tttgccgcgc tgatcgggca aacgcctttg cagcaggcgc tattgctggc tgcgatcgat
     3601 ccgggtctgg gtggcgtgct ggtcagtggg ccgcgcggca cggcgaaatc gaccgcggcg
     3661 cgcgcgctcg ctgagttgtt gccggaaggg caatgggtga atttgccgct cggcgcgagc
     3721 gaagatcgct tgatcggcac gctcgatatc gaaaccgtgt tgcgtgatgg ctccgtgcgg
     3781 ttttcaccgg gcttgctcgc gaaagcgcat cgcggcgtgc tgtatgtcga tgaagtgaat
     3841 ctgctgcccg atgcgctcgt cgatgcgtta ctcgatgcgg ctgccagtgg cgtgaatact
     3901 gtcgagcgcg atggggtctc gcatagtcac gacgcgagtt tcgtgctgat cggcacgatg
     3961 aatccggaag agggcgaatt gcggccgcaa ctgatcgatc gctttggttt gatggtcgag
     4021 ttgcaaaact gttttgagcc gcgggtgcgt cagcagatcg tcaaggcacg gctggcgttc
     4081 gatctcgatc cgcagggttt tcgtgcggga tatgtgcagc aacaggaaac gtatgtgcag
     4141 cggattcgtg ccgcgcgtga agcactgccg caactcgact tcgacgatga cgtgcatgcg
     4201 catgtcagcg cgctgtgtat tgacgcggcg gtcgatgggt tgcgcgccga tcttgtgatg
     4261 ttgcgtgcgg cgcgcgcatt ggcggcgctg gagcaggcgg ctgtggttac gaccgggcac
     4321 gttgagcgag tcgctgaggc ggcgctgatg catcggcgtc acgatcgcga aacgcgtgca
     4381 gatggcgacg cacgtgagag cgaagcgccg cggcagacaa cgggcgacgc ggatgcaacc
     4441 gcttggcgag cttctcagga tgcagcgcca ggcgccgctg ccggcgatag cgattggggt
     4501 tatttgccac cgcagcccgc gggcgtcacg caggtcaaag gcgtgattgc gctcaacgca
     4561 aaaaaacgct gagccatcg
//
LOCUS       DATASH010000402        18353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4807439,
            whole genome shotgun sequence.
ACCESSION   DATASH010000402 DATASH010000000
VERSION     DATASH010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 18353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18353
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4807439"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(763..1185)
                     /locus_tag="VHV99_11175"
     CDS             complement(763..1185)
                     /locus_tag="VHV99_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636035.1"
                     /translation="MAQQKTNPKLEQALTRGDLAIRQANSARATALLRALGKMIIDAS
                     ATIGVEAFTLIPDGDKIYDPTDGLWPQELQVSLDGPVEEADPDEVRTVRLIADDPGTV
                     FRVEWQRADGKIGRQDGGPFATVAFISDVDIPWTDEED"
     gene            complement(1399..1765)
                     /locus_tag="VHV99_11180"
                     /pseudo
     CDS             complement(1399..1765)
                     /locus_tag="VHV99_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432782.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            1844..2008
                     /locus_tag="VHV99_11185"
                     /pseudo
     CDS             1844..2008
                     /locus_tag="VHV99_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432780.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(2346..3398)
                     /locus_tag="VHV99_11190"
     CDS             complement(2346..3398)
                     /locus_tag="VHV99_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015601897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HEX3636036.1"
                     /translation="MEYRRLGNSNVQVSLIGLGTMTWGEQNTEQEAHAQIDYALDHGV
                     NLIDTAEMYPVPPRAETQGSTERYIGTWIAQHRSAREKIVLATKIAGPARQPHNPRHI
                     RGEGNQFDRKNLTEALNDSLKRLQTDYVDLYQLHWPDRSTMTFGRPAYPWVDDAYTVP
                     IEETLSVLAEFVKEGKIRHIGVSNETPWGVAQFLRAAEKLGLPRIVSIQNPYSLLNRT
                     YEVGLSEYAHHDNIGLLAYSPLAFGWLSGKYEGGARPAGARISLFERFQRYSKPQAVQ
                     ATTRYVELAKRHGLSPAQFALAFVNSRPFLTSNLIGATSLEQLKENIASAEVKLSPEV
                     LAEIDKLHELQPNPAP"
     gene            3543..3755
                     /locus_tag="VHV99_11195"
     CDS             3543..3755
                     /locus_tag="VHV99_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HEX3636037.1"
                     /translation="MNPEHLELLVTRVMPYGKYKGRLIADLPGHYLNWFASQGFPPGE
                     IGRLLALMHEIDHNGLSSLIEPLRKR"
     gene            complement(3885..4457)
                     /gene="cysC"
                     /locus_tag="VHV99_11200"
     CDS             complement(3885..4457)
                     /gene="cysC"
                     /locus_tag="VHV99_11200"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEX3636038.1"
                     /translation="MSATRGAVIWMTGLSGAGKSTLAGALHLRLKEAGHASIVLDGDV
                     LRRGLTAGLGFTPEDRSENLRRIAHVAALFMQQGFIAIAAVISPERQHRRMAREIVGA
                     GFVEVFVNAPLAVCEARDVKGLYARARRGEIPHFTGVSGPFDVPLEPDVTIETDRIPI
                     EQAVSRLVAHLTAMGCLPSESGRDGPRHVE"
     gene            complement(4454..6109)
                     /locus_tag="VHV99_11205"
     CDS             complement(4454..6109)
                     /locus_tag="VHV99_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX3636039.1"
                     /translation="MKHDTQSAIASEWRGQGDARAASGELQAALHCFETARSLDPANA
                     INLERVAATFAALNRFPEAVIRYHEAIAFDPRNADLHHGLGWAFEQMHRLEQAVEAYR
                     EAVRLKPGADGSHNNMANCLQALGRFDESHEAYRRAIEAAPQVPLYYRNFVQSKRLGA
                     DDPVFAGLEQLVGVAASFAPANQAEIHFAYAQALSDVGRNDASFDHFLKANALHRPGV
                     HYDEAASLGLFACLPEFLSAEVLAAKRGLGDPSAAPIFIIGMPRSGSTLIEQILASHP
                     QVFGAGERSEFGEALVNCIGRDAGDPLRIDIEALQNLSATQLRPLGADYLRRMHTALP
                     ELRAATPGYARFTDKYPFNFVNVGLIHLALPNARFVHSSRSPLQTCLSIFSRIFHDVP
                     FGYDLGELGRYYRAYDALMAHWQQVLPEGTMIEIKYEELVEDFEVNVRRLLAHCGLEW
                     DERCLSFHQTVRRVSTASAAQVRRPLYKTSVQRWQPQPALLRPLLDGLGPELAPADSF
                     GAHEQAPLIDADAGNAGNAGNATNTADTADTADTAATGTERKS"
     gene            6459..6797
                     /locus_tag="VHV99_11210"
     CDS             6459..6797
                     /locus_tag="VHV99_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636040.1"
                     /translation="MSRRIASIVLVAVAASLTGCVVAPGYDYGYAQPYYPGYGYGYGP
                     AYGPAYGTVNIWGGWGGSCCYYHGGHGYWHGGHGGGWHGGGGWHGGGGGHGGGSSWSG
                     GGGGHGGHGH"
     gene            6679..6999
                     /locus_tag="VHV99_11215"
     CDS             6679..6999
                     /locus_tag="VHV99_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636041.1"
                     /translation="MEGMAEGGTEAVAGTAVVAATAAAVRGAVAVAAMEDMGIDGVLT
                     PCSFGAIALRWCSGIGPMLSGMGRAAKAHDEAFGEHECDAPHVRSRAVRQLDNADVNP
                     HWIQ"
     gene            complement(7082..7285)
                     /locus_tag="VHV99_11220"
                     /pseudo
     CDS             complement(7082..7285)
                     /locus_tag="VHV99_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006025271.1"
                     /note="with GltJLPS and GadC for glutamate and GltJLP,
                     DctA and DcuAB for aspartate is involved in the transport
                     of glutamate and aspartate; incomplete; partial in the
                     middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            7806..9251
                     /locus_tag="VHV99_11225"
     CDS             7806..9251
                     /locus_tag="VHV99_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3636042.1"
                     /translation="MQAPNLSRAADHNPGHGSLIVMLVAAAFFMENLDGTIIATALPQ
                     MAHSFGVHPVDLSLGITSYLLTLAVFIPISGWAADRFGLRTVFGSAVAVFTAASVLCG
                     MTTSLPEFTAARVLQGIGGAMMVPVGRLAVLRATPKDGLMRAIAFITWPGLAAPVIGP
                     PLGGFITTYSSWRWIFYLNLPLGCIGLLLSIMYLDNAKENAGRRFDTLGFVLTGVAGT
                     TLMYAMELLGRSNTQWSEVTGLLAIGVASGGAAWFHVRRAREPVIDLSALRVKTFAVA
                     MGGGSLFRVSISAVPFLLPLLFQVGFGLNAFASGLLTLSVFAGNLSMKVVTTPVMRRF
                     GFRRVLIVNGLFAAISLAAMSFLAPATPRTLIVIVLFLSGLSRSLQFTALNTLSFADV
                     PKTQMSGASALSSTLFQMTMGVGVALGAIALRVAEWLHGHDSHSIAPSDFSVAFLIVA
                     AVGLLGIVDVFGLARDAGAEVSGQAPARRPG"
     gene            complement(9357..10373)
                     /locus_tag="VHV99_11230"
     CDS             complement(9357..10373)
                     /locus_tag="VHV99_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX3636043.1"
                     /translation="MSHTESKPNGARDGRRSLLKMAAGLGAGVALRGLGVAGVTLAAS
                     SAARAQGSKVLSIGYQKYGNFIVLKARGTLEKRLAPLGFSVQWLEFPAGPQLLEGVNA
                     NAVDIGVVGETPPVFAQAGGVNFVYIAAEPDAPRGEALIVPKDSPIRSIAELRGKKVA
                     VTKGSNAHYLLVKALEHGKVGYNEIQQAYLVPADARAAFANGSVDAWSIWDPYLAAAE
                     RQLGARALTNGEGLVRNVEYYVARRSFATEHADVVHAVLAELQQLDGWAEHNVAAVAA
                     QLAPLTGLDTGTLELALGRTGYGVLPVDAGRFAYQQQIADALYEIKLIPKKLAVTDAR
                     WQNA"
     gene            complement(11287..11362)
                     /locus_tag="VHV99_11235"
     tRNA            complement(11287..11362)
                     /locus_tag="VHV99_11235"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11327..11329),aa:His,seq:gtg)
     gene            complement(11467..12468)
                     /gene="dusA"
                     /locus_tag="VHV99_11240"
     CDS             complement(11467..12468)
                     /gene="dusA"
                     /locus_tag="VHV99_11240"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007588016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HEX3636044.1"
                     /translation="MSSAQPSSPRRVSVAPMMDWTDRHCRSLHRVISRHAWLYTEMVT
                     TGALLHGDVPRHLAFTPDEAPVALQLGGSEPDDLARSAKLGEQWGYDEINLNCGCPSE
                     RVQRGAFGACLMNEPQLVADCVKAMRDAVAIPVTVKHRIGVDAVEDYAFVRDFVGTIA
                     EAGCNVFVVHARNAILKGLSPKENREIPPLKYDYAYQLKRDFPHLEIIINGGIKTLDE
                     VETHLQHVDGVMLGREAYHNPYVLADVDARFYGSTDAPLTREQVEAKLIDYCAAEMAR
                     GTYLGSITRHALGLYRGEAGARGWRRVLSDSKRLAARDLTIFDEARQHLREPVEIFE"
     gene            12821..13090
                     /locus_tag="VHV99_11245"
     CDS             12821..13090
                     /locus_tag="VHV99_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636045.1"
                     /translation="MKTKLIAALLVAVSASIAAPAFASGYGPAPYYRPSVGAPASQQG
                     LNAQTVAAERNSAEANSYGGVNNVSSQSGTRAQPSSGPQSVFFGH"
     gene            complement(13221..13601)
                     /locus_tag="VHV99_11250"
     CDS             complement(13221..13601)
                     /locus_tag="VHV99_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase superfamily protein"
                     /protein_id="HEX3636046.1"
                     /translation="MHLARAIPIIRIFSEEKAREFYVDFLGFTIDWEHRFEANFPLYM
                     QVRRSELILHLTEHHGDATPGSTIFVPAQDIGALHAELQRKDYKYGKPGVEVLPWGNV
                     LETIDPFGNRIRFCEAPQEDAREA"
     gene            complement(13776..13973)
                     /locus_tag="VHV99_11255"
     CDS             complement(13776..13973)
                     /locus_tag="VHV99_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636047.1"
                     /translation="MLKSLSPEAVEALRRLNDAGVGQPVPKFAQSVAAELLTNGLAAK
                     ASGGVVEITCNGRQYLSGDCD"
     gene            14243..14518
                     /locus_tag="VHV99_11260"
     CDS             14243..14518
                     /locus_tag="VHV99_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636048.1"
                     /translation="MERYSMTALTAGVALAIVGACGTVAFAQTSNPALKTQAAPQNGG
                     ASMVKPPEAASGSGGASNPDNMPIKRPRKPTNDKMMHAPPASAANAK"
     gene            complement(14522..14737)
                     /locus_tag="VHV99_11265"
     CDS             complement(14522..14737)
                     /locus_tag="VHV99_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007588023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEX3636049.1"
                     /translation="MSISAINVRNQFKGKVKEIIRGPVVSEVDVDTPFGIVTSVITTR
                     SVDELDLKVGSEVVALVKSTEVSIARL"
     gene            complement(14792..15793)
                     /locus_tag="VHV99_11270"
     CDS             complement(14792..15793)
                     /locus_tag="VHV99_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018432126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3636050.1"
                     /translation="MNATTLSASFGGVAGSDLEAGRAQPHAVDHDAHEAAGFEHAGTA
                     ARDSRRAGSVPAAGAPSRNDACRSADYSVELRGVGKQYGEREVLADFDLSIERGSFVA
                     IVGRSGCGKSTLLRLVAGLEKATSGVLEKRAEDGRPLDTRIMFQDARLLPWKSVLQNV
                     MLGLGRSAREDARAVLAEVGLLERANDWPAQLSGGQRQRVALARALVHRPQLLLLDEP
                     LGALDALTRIEMHALIERLWREHQFTALLVTHDVHEAVALGERILLIEDGRIALDQPV
                     PLARPRARASAGFAALEEHVLKRVLKTAPNDDALSDRAYDARDESRLTRPTDVRWAV"
     gene            complement(15790..16656)
                     /gene="ssuC"
                     /locus_tag="VHV99_11275"
     CDS             complement(15790..16656)
                     /gene="ssuC"
                     /locus_tag="VHV99_11275"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HEX3636051.1"
                     /translation="MAQSAVSAQRRTQAGAQAGSSARPEALVGLLRRIGAHLAPWLAP
                     LAILLAWEFAARSGLLSTRVLPEPLAVVKAAWSLIQSGEMWADVKVSTWRAVSGFAIG
                     GSIGLVLGLATGLFKPVEIALDSTVQMIRNIPALAMIPLVILWFGIEEEAKLFLVALG
                     VFFPIYVNTFHGIRSVDANLIEMARSYGVKGFALYRHVILPGALPSILVGVRFALGLM
                     WVTLIVAETISAQSGIGYMTMNAREFLQTDVVVVGILLYAVLGKLADVLAKGLERVTL
                     RWHPAYQRGAKA"
     gene            complement(16712..17872)
                     /gene="ssuD"
                     /locus_tag="VHV99_11280"
     CDS             complement(16712..17872)
                     /gene="ssuD"
                     /locus_tag="VHV99_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HEX3636052.1"
                     /translation="MNVFWFIPTHGDSRYLGTSHGARAADFDYFQQVAVAADTLGYEG
                     VLLPTGRSCEDAWVVASSLIAATKRLKFLVAIRPGLSSPGLAARMAATFDRLSNGRLL
                     INVVTGGGPAELEGDGIFVDHDTRYEITDEFLHIWRKLLSAAHTNDAIEFNGKHLTSK
                     GGKSLYPPVQNPHPPLWFGGSSAAAHNIAADHIDTYLTWGEPPAAVAKKIADIRARAE
                     ARGRKIKFGIRFHVIVRETEEEAWAAADKLISKLDDETVARAQASFSKMDSEGQRRMS
                     ALHGGKRGGRAELEVYPHLWAGVGLVRGGAGTALVGNPEQVAALMKQYAELGIDTFIL
                     SGYPHLEESYRFAELVFPLLPGHQTKKASGPLSGPFGEIVGNDYLPKAASSS"
BASE COUNT         3566 a         5818 c         5518 g         3451 t
ORIGIN      
        1 gttggccttt ccttgatctg actctttggc ctttccttga attgttattg gtctattagc
       61 gttgcccctg tgcggggcgg cacttacttt ctttgccgcc gcaaagaaag ataagcaaag
      121 aaagcggctc accccgccag ctcataagcg ggcccctcgc acagttccgg tagtggtgca
      181 tctggaatcc gttccctcgc acattccgcc tcggtgacca ggcagtcata cttccggcgg
      241 cgctacgcgc gccgaagcgc atttcataaa accaaccacc acgctttcgc gcttgcgctt
      301 cattcggcgc ggtggagcgg cgcgcattgc ctcttcgccg ttctctcgcc gctgtcgttt
      361 gggcgcctcg ccgacgcgaa gccgatgccc caccacgcac aaacgaatcc ctggtttcct
      421 gtgcataccc atccgcgacg cacgtagagc ggagtgggcg ctgatgagcc ctttgtcact
      481 caggccaaat gtgcgagaac acggattcca gatgcaccac taccgcaact gtgcggggga
      541 cccgcttatg agctggcggg ttgagccgct ttctttgctt agctttcttt gcggcggcaa
      601 agaaagtaag tgccgccccg cacaggggca acgctaatag accaacatca cttcaaggaa
      661 aggccaaaaa tccagttcag acaaagtcca agaagtcaga tcaatcaaag tccaacgccg
      721 taggcaacca cccaaacccc agcgccccgc gcaacttcaa cttcaatcct cttcatccgt
      781 ccacggaata tccacatcgg aaatgaaagc caccgttgca aacggccccc catcctgacg
      841 cccgatcttc ccatcagccc gctgccattc caccctgaac accgtgccgg gatcatcggc
      901 aatcaaccgc acagtccgaa cctcgtccgg atccgcttcc tcgacaggac cgtccagcga
      961 aacctgcaat tcctgcggcc acagcccgtc ggtgggatcg taaatcttgt cgccgtccgg
     1021 aatcagcgtg aatgcctcca cgccgatcgt cgccgacgcg tcgatgatca tcttgcccaa
     1081 cgcgcgcagc aacgcagtcg cccgcgccga gttggcctga cgaattgcaa gatccccgcg
     1141 cgtcagcgcc tgctcaagtt ttgggtttgt tttttgttgc gccattcgat gtcctgagtc
     1201 ctatcgctct gttgtggacg gcacgcccat tcatcgcgca ccgctctcca tttttcgggc
     1261 tgaatcctac cactggtgtg agccacagcg gcacaagccg cgcagcagag gccgaccagc
     1321 ccgccccgtc cccacagttt aggtcgtgat tcacgctccg gcagggagat ttgcctttca
     1381 atgcgtctcg ccacgccttc aggcagcctt cggcgccgca gcatccgcgg actgcgcgcc
     1441 gccgatctcc ccgatcagcg ggtcgcggaa agccttcacc gacggcggca gttcccctcc
     1501 ggccatcgtc tgctcctgaa tgctgcgctg acgcatttgc tgatacgttg gcggtatcgc
     1561 gacgaagccg tcgtcaagct aacggtcgcg taccgacagc agccccgtaa aacatgaccg
     1621 cccccgaatt tctgagcata gcggtacatc gcgccgctat cgttgcagct gagatcgggc
     1681 tcgaccagca cgcctcgagc gcgcaggtaa tgtcgatcag ctgacgaatc gtcgtgccaa
     1741 ggtccggccg cgccagcgga tggcgccgcc gacgatctgc gcgattctgc cggctgaccg
     1801 ccgaaacggc cacatggagc ggcgcgaccg gtctcgcggt caacacaaca tcggcgtgac
     1861 ggtgttcggc ggctttgcgc ccttcgcgat caccgggctg atcgaggcaa gcggcaataa
     1921 actcgcaccc ggtttctatc cgatattctg cgtggtggcg agcttactgg cgctctttgc
     1981 ggtgtgctcg aagcggaaga atcgctgagc aaatttgaga ttggcgatcc tcgcgaaacg
     2041 cgatcagcgc aagctggtgt tgatgagcct cgcgaagcgg atcagcctag tttggtgttg
     2101 acgagcctcg cgaagcgcga tcaatgcagt ttggtgttga cgagcctcgc ggagcggatc
     2161 agcccagttt ggtgttgaca agcctcgcga agcagatcgg cgcagtttgg tgttgacgag
     2221 cctcgcgaag cgcgatcaac ccagtttggt attgatgaac ctcgcgaagc ggatcacctc
     2281 atgctcgtgc cccggcaacg aagccgggac accacccggt caaactcgtg ccgactcaat
     2341 acgcctcaag gcgccggatt cggctgcaat tcgtgcagct tgtcgatctc cgcgagcact
     2401 tccggcgaga gtttcacctc ggcgctggcg atgttttcct tcagttgctc gagcgacgtg
     2461 gcgccgatca gattgctcgt caagaacggc cggctattga cgaaggccag cgcgaattgc
     2521 gcgggcgaca aaccgtgacg cttcgccagt tccacataac gcgtggttgc ctgaaccgct
     2581 tgcggcttgc tgtagcgctg aaagcgctcg aacagcgaga tgcgggcgcc tgccgggcgc
     2641 gcgccgcctt catacttgcc cgacagccag ccgaaggcca gcggcgaata cgcaagcagg
     2701 ccgatattgt cgtgatgcgc gtactcggac aggcccactt cgtaggtgcg gttcaacagg
     2761 ctatacggat tctggatact gacgatacgc ggcaagccga gtttttccgc cgcgcgcaga
     2821 aactgcgcga caccccaagg cgtttcgttc gacacgccaa tatgccgaat tttgccttcc
     2881 ttgacgaatt cggcgagcac cgacagcgtt tcttcgatcg gcacggtgta cgcgtcgtcg
     2941 acccacggat aggcgggacg gccgaacgtc atcgtgctgc gatccggcca gtgcaactga
     3001 tacaggtcga cgtagtccgt ttgcaggcgc ttgaggctat cgttcagggc ttcggtcagg
     3061 ttcttgcggt cgaactggtt gccctcgccg cgaatatggc gcggattgtg cggttgacgc
     3121 gccgggccgg cgatcttggt ggcaagcacg attttctcgc gcgcgctgcg gtgctgcgcg
     3181 atccacgtgc cgatgtagcg ctcggtcgag ccttgcgttt cggctcgcgg cggcaccggg
     3241 tacatttccg cggtgtcgat cagattgacg ccgtggtcga gcgcgtagtc gatctgcgcg
     3301 tgcgcttcct gctcggtatt ctgctcgccc cacgtcatgg tgccgagacc gatcaggcta
     3361 acctgcacat tcgagttgcc gagtctgcgg tattccattg aacttgtcct gttgaatatg
     3421 tgaacggaaa gcgcgtgacg ttaccatgcc cgcgcaatgc ctgcagcgca gctaaaatgt
     3481 tgttcccgca ggcgtggcgc acttcgccgc ccttaccgat tttctccgtc gacccatctg
     3541 cgatgaatcc cgaacacctc gaactactgg tcacacgcgt gatgccttac ggaaaataca
     3601 aaggccggct gatcgcggac ctgccgggtc actacctgaa ctggtttgcg agccagggct
     3661 ttccgccggg cgaaatcggc cgtctgctgg cgttgatgca cgagatcgat cacaacggtt
     3721 tgtcgtcgct gatcgaaccc ttacgcaaac gctgaggcaa gcgccgagcg cggcaccttc
     3781 ggcgcacgcc agccgaggat gacggcgagc acgcccagaa tgaaacccgc cgcagcgccc
     3841 aacggagatt ttgcgcggac aaaaaagcac ccgcttggcg ccggttattc cacgtgacga
     3901 ggcccgtcgc gcccgctttc gctgggcagg cagcccatcg cggtgagatg ggcaacgagg
     3961 cggctgaccg cctgctcaat cggtatccga tcggtttcga tcgtgacatc cggctcaagc
     4021 ggcacgtcga agggcccgga aacaccggtg aaatggggaa tctcaccgcg ccgcgcccgc
     4081 gcgtacaggc ctttgacatc gcgcgcttcg cacaccgcca gcggcgcatt cacgaacact
     4141 tcgacgaacc cagcgcccac gatctcgcgc gccatgcgcc gatgctgacg ctcgggcgag
     4201 atcaccgcgg caatcgcgat gaagccctgt tgcatgaaca gcgccgcgac atgcgcgatc
     4261 cgccgcaaat tctcgctgcg gtcctcgggc gtgaagccga gaccggcggt caggccgcgc
     4321 cgcaggacgt cgccgtcgag cacgatcgag gcatgccccg cttctttcag acgcaggtgc
     4381 aacgcgccgg ccaaggtcga tttaccggcg ccggacaagc cggtcatcca gatgacagct
     4441 ccacgggtcg cgctcatgac ttgcgctccg tgccggtagc tgccgtatcg gctgtatcgg
     4501 ccgtatcggc tgtgttggtc gcattgcccg cattgcccgc attgcccgca tcagcatcaa
     4561 taagcggcgc ctgctcgtgc gcaccgaagc tgtcagcggg cgccagctcc ggtccgaggc
     4621 catcgagcaa cggtcgcagc agcgcgggct gcggctgcca gcgctgcacc gacgtcttgt
     4681 agagcggacg gcgcacctgc gccgcgcttg ccgtactcac ccgccgcacg gtctgatgaa
     4741 acgacagaca gcgctcgtcc cactcgagtc cgcaatgcgc gagcaagcgc cggacattca
     4801 cttcgaagtc ttcgaccagt tcctcgtatt tgatttcgat catcgtgccc tcgggtaaca
     4861 cctgttgcca atgtgccatc aacgcgtcgt acgcgcgata gtagcggccc agttcaccca
     4921 gatcgtagcc gaacggcacg tcgtgaaaaa ttcgcgaaaa aatcgacagg caggtttgca
     4981 gcggcgagcg gctgctgtga acgaagcgcg cattcggcaa cgccagatga atcagcccga
     5041 cattgacgaa gttgaacggg tatttgtcgg tgaaacgcgc atagcccggt gtggcggctc
     5101 gcagttccgg cagcgccgtg tgcatccggc gcaaatagtc ggcgccgagc gggcgcaatt
     5161 gcgtggcgct caagttctgc aacgcttcga tatcgatgcg caacggatcg cccgcatcgc
     5221 gtccgatgca attcaccaac gcctcgccga actccgagcg ttcaccggcg ccgaaaacct
     5281 gcggatgact cgcgaggatt tgctcgatca gagtcgaacc ggagcgcggc attccgatga
     5341 taaaaatcgg cgccgccgac ggatcgccca gaccgcgctt cgctgccagc acctccgcac
     5401 tcaggaattc cggcaaacac gcgaacaggc cgagcgacgc ggcttcgtca tagtgcacgc
     5461 cggggcggtg caacgcattg gctttcagga aatgatcgaa cgacgcatcg ttgcgcccca
     5521 catcggagag tgcctgagca tacgcgaagt gaatctccgc ctgattggcg ggtgcgaacg
     5581 aagcggcaac gcccacgagc tgctcgagcc cggcgaaaac cggatcgtcg gcgccaaggc
     5641 gtttggactg cacgaaattg cggtaataca acggcacctg cggggcggcc tcgatcgcgc
     5701 ggcgataggc ctcgtgcgac tcgtcgaacc ggccgagcgc ctgcaaacag ttggccatgt
     5761 tgttgtgcga gccgtcggcg cccggcttca gccggactgc ttcgcgatag gcctctaccg
     5821 cctgctcgag ccggtgcatc tgctcgaacg cccagccgag gccgtgatgc aaatcggcgt
     5881 tgcgcggatc gaatgcgatc gcttcgtgat agcggatcac ggcttcggga aagcggttca
     5941 gcgcggcgaa cgtcgcggcg acacgctcaa ggttgatggc attggcagga tcgagcgagc
     6001 gtgctgtttc gaaacaatgg agtgcagctt gcagttcgcc gctagcggcg cgggcgtcgc
     6061 cttggccacg ccattctgat gcgattgccg actgtgtatc gtgcttcatt gacggccaga
     6121 tttttgcagg cttggcatgg agcggcccgg caaggggaag tgcgttcttc ttcgtccagc
     6181 cgccgcgcaa gcgtgcgact ggcctcaaag gcaaattgtc tgacgggaaa gtatcaggcg
     6241 caccctacag ttacgattcg aaaatttaac caactgttac acaaggatgc atccgtcagg
     6301 gttcgaaaga ctgccgcagc gcgcggcccc atggcattcg attgccgcgc gcatgtcagc
     6361 gaagcgcgcg ggcgcagccg cgtcgtaatg tgcggacaca aatgctgctg aactccggga
     6421 cgcgccgcat ttctcactca catacgcggg agaaaatcgt gagcaggcgc attgcatcaa
     6481 tcgtattggt ggctgtggcg gcaagtctga cggggtgcgt agtcgcgccg ggatacgact
     6541 atggctacgc gcaaccctat tatccgggct acggttatgg gtacgggccg gcgtatggac
     6601 cggcttacgg cacggtgaac atttggggcg gctggggcgg ctcgtgctgt tattaccacg
     6661 ggggccacgg gtactggcat ggagggcatg gcggagggtg gcacggaggc ggtggctggc
     6721 acggcggtgg tggcggccac ggcggcggca gttcgtggag cggtggcggt ggcggccatg
     6781 gaggacatgg gcattgacgg ggtgctgacg ccatgttcgt ttggcgcgat cgcgcttcga
     6841 tggtgttcag gaatcggacc gatgctcagc ggcatgggcc gcgctgccaa ggcgcacgac
     6901 gaagcctttg gcgaacacga atgcgatgcg ccccatgttc gttcgcgtgc cgttcgacaa
     6961 cttgacaatg ccgatgtgaa tccgcattgg attcaataat gaggcgaagc ccccctcctc
     7021 gcgcgaagac ataccgcctg aatggcctcc gtgcccgctg cgccccccca cgccggaccg
     7081 gcaatcgacg cactacggcg gacattgccg tgcacacaca gaagtagcat gcgccggcaa
     7141 acaagaccaa ctcgactgtc gtgccatcgc gtgccccaac gttcgaggca atcgtgaaga
     7201 agtcgccgag gccaatcaca tagacgagag acgtgtcctg aaacaggatg atggcctgcg
     7261 tcagcagcaa cgggacgatc gcccgcacct gctcgcatac ccgcgccgac gtgaacaacc
     7321 caaggcagat tatcgacgtc agcacgccgg atttgctccg gcggcatatg cctgagcgta
     7381 ctgccgagcg ggccaggagc agctcgggaa cggcccagaa ccacaggaag aactgaacga
     7441 tcaacggaat gttgtgaaac agcgcgacat agcaatcgaa gcgtgttctt ccgaatcatt
     7501 gaaaatggtc tgcaagagta cagttcgcgt tagttgcgaa tgcaagcaac ttgcaagtag
     7561 atctcaatca cgctggcgac ggcaagtatt tacagtggat ttgctattcg gtctttctgc
     7621 taagcctcag accagccatg cgcctcgacg agtcgaatca tgtgtaattt tcgctaaccg
     7681 aatcgttgat tgctatttgc agcaaatccc ttttgattct attgattgtg cggtgcggct
     7741 cacgtacgct gtggcccgaa tcccggccaa tccgccagtg aataacgttc aggagatcct
     7801 cgccgatgca ggcccccaat ctatcccgcg cggcggacca caacccagga catggctcgc
     7861 tgattgtgat gctggttgcg gccgcttttt tcatggagaa cctcgacggc accatcatcg
     7921 ccacggcgct gccgcagatg gcgcactcgt tcggcgtgca tccggtcgat ctgagtctcg
     7981 gcatcacctc gtatttgctg acgctcgccg tatttatccc gatcagtggc tgggccgccg
     8041 accgctttgg cctgcggacc gtgttcggca gcgcggtcgc cgtgttcacc gcggcgtcgg
     8101 tgctgtgcgg catgaccacg agcctgcccg aattcaccgc tgctcgcgtg ctgcaaggca
     8161 tcggtggcgc catgatggtg ccggtcggcc ggctcgccgt gttgcgcgct acgccgaagg
     8221 acggcctcat gcgcgctatc gccttcatta cctggccggg actcgccgcg ccggtgatcg
     8281 ggccgccgct tggcggcttc atcacgacct actcgtcgtg gcgctggatc ttctacctga
     8341 acctgccgct cggctgcatc ggtttgctgt tgtcgatcat gtatctcgac aacgcaaagg
     8401 agaatgccgg ccgccgcttc gatacgctcg gttttgtact gacgggcgtc gccgggacga
     8461 cgctgatgta cgcgatggaa ttgcttggac gcagcaatac gcagtggtcc gaggtgacgg
     8521 gcctgctcgc catcggcgtg gcctcgggcg gcgccgcatg gtttcatgtg cggcgcgcgc
     8581 gcgagccggt catcgatctg tcggccttgc gtgtgaagac ctttgcggtg gccatgggcg
     8641 gcggctcgct ctttcgcgtg tcgatcagtg cagtgccgtt tctgctgcca ctgctgtttc
     8701 aggtgggctt cggactgaat gcgttcgcgt cggggctgct cacgctctcg gtgtttgccg
     8761 gcaacctgtc aatgaaggtg gtgacgacgc ccgtcatgcg gcgcttcggc tttcgccgcg
     8821 tgctgatcgt caacgggctg ttcgccgcga tttcgctcgc ggcaatgagt ttccttgcgc
     8881 ctgccacgcc caggacgctg atcgtgatcg tgctcttcct gagcgggctg tcgcgttcat
     8941 tgcagttcac ggcgttgaat acactcagct tcgccgacgt cccgaagacg cagatgagcg
     9001 gcgcatccgc gttgtcgagc accttgttcc agatgacgat gggtgtcggc gtggcccttg
     9061 gcgcgatcgc gctacgcgtc gccgaatggc tgcatggtca cgattcgcat tcgatcgcgc
     9121 cgtcggattt cagcgtcgcg tttctgatcg ttgcggccgt gggcttgctc ggcatcgtcg
     9181 atgtgttcgg gcttgcgcgc gatgcagggg ccgaggtcag cgggcaagcg ccagcgcgcc
     9241 gccctggata gcaaacggta ttcgcacaat caggccggca ggcccgctcc ccggcgcggc
     9301 acgaggccgc gcagcgtgac gtgcgagccc ggacccgcac gcgctacgcg cctgcatcat
     9361 gcgttttgcc agcgcgcgtc ggtgacggcc agtttcttcg ggatcagctt gatctcgtag
     9421 agcgcgtcag cgatctgctg ctgatacgcg aagcggcccg cgtcaaccgg cagcacgccg
     9481 taacccgtgc ggcccaacgc gagttcgagc gtgcccgtgt cgagcccggt aagcggcgcg
     9541 agctgggcgg cgacggccgc cacgttgtgc tcggcccagc cgtcgagctg ctgcagttcg
     9601 gccaacacgg cgtgcaccac atcggcgtgc tccgtcgcga acgaacggcg cgcgacataa
     9661 tattcgacgt tgcgtacgag gccctcgcca ttcgtgagcg cgcgcgcccc cagctgacgc
     9721 tcggcggcgg caagatacgg atcccagatc gaccaggcgt cgacactgcc gttggcaaac
     9781 gcggcgcgcg catcggcggg aacgaggtag gcctgctgaa tctcgttata gccgaccttg
     9841 ccgtgctcca gcgccttgac caacaggtag tgggcattcg agcccttggt gacggccacc
     9901 ttcttgccgc gcagctcggc gatcgagcgg atcggcgagt ccttcggcac aatcagcgct
     9961 tcgccgcgcg gtgcgtccgg ctcggcggcg atgtacacga agttcacgcc gcccgcctga
    10021 gcaaacacgg gcggcgtctc ccccaccacg ccgatatcga cggcgttcgc attcacgcct
    10081 tccagcagct gcgggcccgc cggaaactcg agccactgaa cggaaaagcc gagcggcgcg
    10141 agacgctttt caagcgtgcc gcgcgccttg agaacgatga aattgccgta tttctgataa
    10201 ccgatgctca gcaccttgct gccctgcgcg cgtgccgcgc tactcgcggc aagcgtcaca
    10261 ccagcgacgc cgagtccacg cagggccacg cccgcgccaa ggcctgcggc catcttcagt
    10321 aacgaacggc ggccgtcgcg cgcgccgttg ggcttcgatt cggtatgaga cataaggcgg
    10381 aggtcctgag aagatcagta agccatgggc gcagaaaagc ggtgccgtta tagccacagc
    10441 ttcgacgtat gaatgagggc gaataatgcg caatggtcaa tgcgccgtca aagaatcgtt
    10501 tctgctatag atcgaggata tgcgcatatg tgccgtcgcc agcatttgcg ctggcgtgag
    10561 ttgggatctc ctcaccgtcc tgtcgagccg gcgatcgccg cgcagtgagc tgtttcgcgg
    10621 aattcatctg ttcgccgttg atcaattcta tgaaccggca acataagcag tactgcgcat
    10681 atcgatcaac tttccttcgc gagccttact gccacaaaac ctgtgtatgg ctatctcgac
    10741 aaacgcgctc aactcggcgc ggcgacgaag gcaaacaagg aaatagcgct tcagtcagcg
    10801 gtgctcgagt aggggcgtcg gcgcggtgag atcgaggtca acccgtgccg cggaattgaa
    10861 gacaatccaa cgcggccacg tacacgctat gtgcatccgc acgagatcgc gctggccatt
    10921 gaggtcgcgc gttcgcgaaa gagcgtcgtg acaagcaccc gaactcgacc tgtctgatcc
    10981 tggcgttgtg cgttcaaacg gcctatctgg cggtcagtcg cctgaccgag atacgtgagt
    11041 ggcgccggca gtcgagagac gctgacggca tcaccgcgcc gatcgccaag cgaaaggctg
    11101 gcaagccaat atttggcctg ccgagtcaag acctaccgct gcatcgtcct cgacgttcaa
    11161 ttagacgaaa aaaagcccgc tataagcggg cttaattttc aagctcatct tccaaaaatg
    11221 gaatttcatc ttccaacagt tggaacaagc cagtaactac tttcgcggta atccgcatga
    11281 attctttggg gtggctgatg gggctcgaac ccacgacaac aggaatcaca atcctggact
    11341 ctaccaactg agctacagcc accactgaca tctgcttaac cttgctaagc aactcattgc
    11401 tgagaaactt ggctcgccga tgaagaaacg agattatacg aacaagaatt ccgtttgcct
    11461 agtcctttat tcaaaaattt cgacgggctc acgcagatgc tgtcttgcct cgtcgaagat
    11521 ggtcagatca cgcgccgcca gacgtttgct gtcggacaac acgcgacgcc atccacgcgc
    11581 acccgcttca ccgcggtaaa gaccgagcgc atgacgcgtg atcgaaccaa ggtacgtgcc
    11641 gcgcgccatt tcagccgcgc aatagtcgat cagcttcgcc tcgacctgct cgcgcgtgag
    11701 cggcgcatcc gtcgagccgt agaaacgtgc atcgacatcg gccagcacat aagggttgtg
    11761 ataagcctcg cgcccgagca tcaccccgtc gacgtgctgg agatgcgtct ctacttcatc
    11821 cagcgtcttg atgccgccat tgatgatgat ctccagatgc gggaaatcgc gcttcaactg
    11881 atacgcgtag tcgtatttga gcggcggaat ctcgcggttt tctttcgggc tcaagccctt
    11941 gagaatcgcg ttgcgcgcgt gcacgacaaa cacgttgcag ccggcctccg cgatcgtgcc
    12001 gacgaagtcg cgcacaaagg catagtcctc caccgcatcg acgccgatcc ggtgcttgac
    12061 cgttaccggt attgccacgg catcgcgcat ggccttcacg caatcggcaa cgagttgcgg
    12121 ttcgttcatc agacacgcgc cgaacgcacc gcgctgcaca cgctcagatg ggcagccgca
    12181 gttcagattg atctcgtcat agccccactg ctcaccgagc tttgccgagc gagcgaggtc
    12241 gtcaggttca ctgccgccga gttgcaaggc gaccggcgct tcatcgggcg taaacgcgag
    12301 atggcgcggc acatcgccgt gcagcagcgc gccagtggtg accatttcgg tgtagagcca
    12361 cgcatgccgc gatatcacgc gatgtaaaga gcggcagtgg cgatcggtcc agtccatcat
    12421 cggcgcgacg gaaacacggc gcggactgga aggttgagct gaagacatgg ggcggccagg
    12481 cggcttgaat caaaccgcga ttttaccgca agtcgtcaaa tgccccggaa acgcccccag
    12541 gacagggcag aaacttccac caacaccgcg ataacccacc ctgccaggcg ggtctttgaa
    12601 cgccaaaaac gtttcgccga cggggtcaga acacggcaaa ggcttgttgc atatacgtct
    12661 gaccgattat ttcagtaacg agaatagtaa tttcagatta tcagcataaa ttttcgatag
    12721 taggggtata cactgcattc catcgacgtt gcagacaact cctcacaggc ccctgcaacg
    12781 ctttcctgaa atgaaaccga aacgttctcg gagttacacc atgaagacca agctcatcgc
    12841 tgccctgctg gttgctgttt ctgcctcgat cgctgctccc gcattcgcta gcggctacgg
    12901 cccggcaccg tactatcgtc cgtcagttgg cgcaccggct tcgcaacaag gcttgaacgc
    12961 gcaaactgtt gcagcagaac gcaatagcgc cgaagcgaac tcgtacggcg gcgtgaacaa
    13021 cgtgtcgtcg caatcgggta cgcgtgcaca accttcgtcg ggcccgcaat cggttttctt
    13081 cggccactaa tctgtcgccg ttgaggggtg ccgttcacaa gacagcagcc ccgaactggg
    13141 cgccggcatg cagcaatgca tgccggcgcc cattcccgtt tacagggcgg ccgcgacaac
    13201 gagccccgcg ccctccttcg tcacgcctcg cgcgcatcct cttgcggcgc ttcgcagaaa
    13261 cgaatccggt taccgaacgg atcgatcgtt tccagcacgt tcccccacgg caacacttcc
    13321 acacccggct tcccatactt gtagtccttg cgttgaagtt cagcatgcaa cgcgccaatg
    13381 tcctgcgccg gcacgaaaat cgtcgagccg ggcgtcgcgt cgccatgatg ctcggtcaag
    13441 tgcaggatca gctcggagcg gcgcacctgc atgtagagcg ggaaattggc ctcgaagcgg
    13501 tgttcccagt cgatggtgaa gccaagaaag tccacgtaaa actctctggc tttctcttca
    13561 gagaaaatac ggataatcgg aatggcacgt gcgagatgca aagcggcctc cagcgagtcg
    13621 acgagaatcc gtcagcttag cgaatacgct ggccctttgg cgccacgttc gcctcctggg
    13681 tgacttccac gccgttggtg aaattccgtg ggcatgtttg tccgccgggc cgcccgcacg
    13741 gctcggaacg cgcactgggc cgcctcatcc gacgatcaat cgcaatcgcc ggaaaggtac
    13801 tgccggccgt tgcaggtgat ctcgactacc ccgccgctcg ctttggcagc gagtccattc
    13861 gtcagcaatt ccgcggcgac ggattgcgcg aacttcggca cgggctgtcc gacaccggca
    13921 tcgttcaggc gccgcaatgc ttcaaccgct tcaggactca atgacttcaa catggccgtc
    13981 tccgtcgtga tgctcaattc atcctagcaa gtttcgcggc cgcatgcagt ggcgcgtcga
    14041 ggcataggcg ttgcgtgcgg aacgttgcgc ggtggcgctc cgtgcgcgcc accacgtgcc
    14101 cggcatcgta ttgattggat gtggactgga ttcgcccggg gcggaacaca gcgcaactcg
    14161 cgaattttcc gcgcgctccc gggtctaccc cacaagcgtg gacgcagaaa atcccataag
    14221 ccgcaccgga aaggagagcg agatggagag atacagcatg acagcgctga cggcgggcgt
    14281 cgcgctcgcg atagtcggcg cgtgcggcac cgtggcgttc gcgcaaacgt caaacccggc
    14341 gctcaagacg caagcggcac cgcaaaatgg cggcgcttcg atggtcaagc cgcccgaggc
    14401 ggcctccggc tcaggcggtg cgagcaatcc ggacaacatg ccgatcaagc gtccgcgcaa
    14461 accgaccaac gacaagatga tgcacgcgcc gcccgccagt gccgccaacg cgaaatagcg
    14521 cttacagacg cgcgatcgac acctccgtcg acttcaccag cgcgaccact tccgagccga
    14581 ccttcaggtc gagttcgtcg accgaacgcg tggtgatcac cgaggtaacg ataccgaacg
    14641 gcgtatccac atcgacctcg gacaccaccg gcccgcggat gatttccttg accttgcctt
    14701 tgaactgatt acgtacgttg attgcggaaa tgctcatgtc gagtggctcc agaaagcgat
    14761 tgaaaacgtg aaaacggtca gtcaagggat atcagacggc ccagcggacg tctgtcggcc
    14821 gcgtgaggcg gctctcgtca cgtgcgtcgt aagcgcgatc ggacagcgcg tcgtcgttcg
    14881 gcgcggtctt caggacgcgt ttcaacacat gttcttccag cgcggcaaaa ccggccgacg
    14941 cacgcgcccg cgggcgcgcc agcggcaccg gctgatcgag cgcgatgcgc ccgtcttcga
    15001 tcagcagaat ccgctcgccc agcgcgacgg cttcatgcac gtcgtgcgtc accagcagcg
    15061 cggtaaattg atgttcgcgc cacaagcgtt cgatcagcgc gtgcatttcg atgcgggtca
    15121 acgcgtcgag cgcgccgagc ggttcgtcca gcagcagcaa ttgcggccga tgcaccagcg
    15181 cccgcgccag cgccacccgc tgacgctgac cgccggacaa ctgcgcgggc cagtcgttgg
    15241 cgcgttcgag caagccgact tcggcgagga ccgcacgcgc atcttcacgg gcgctgcggc
    15301 cgaggccgag catcacgttt tgcagcacgc tcttccaggg caagagccgc gcgtcctgga
    15361 acatgatgcg ggtgtcgagc ggccggccgt cttcggcgcg cttttcgagc acgcccgagg
    15421 tggccttttc cagacccgcg accaaccgca acaaggtcga tttcccgcag ccgctgcgcc
    15481 caacaatcgc cacgaaactg ccgcgctcga tcgacagatc gaaatcggcc agcacctcgc
    15541 gctcgccgta ctgcttgccc acaccgcgca actcgaccga atagtcggcg gatcggcatg
    15601 cgtcgttgcg gcttggcgcg cccgccgccg gcaccgagcc ggcgcgtcgg ctatcgcgcg
    15661 ccgccgtacc ggcgtgctcg aaacccgcgg cttcgtgcgc gtcgtgatcg acggcgtgag
    15721 gttgcgcccg tccggcttcg agatcgctgc ccgcgacgcc gccgaacgac gccgacaagg
    15781 tcgttgcgtt catgctttgg ctcctcgttg ataagccgga tgccaacgca gcgtgacgcg
    15841 ctcgaggccc ttggccagca cgtcggcgag tttgccgagc accgcgtaca gcagaatgcc
    15901 gaccaccacg acatcggttt gcaggaattc gcgtgcattc atcgtcatgt agccgatgcc
    15961 cgactgcgcg gaaatggttt cagcgacgat cagcgtgacc cacatcagtc ccaacgcgaa
    16021 acgcacgccg accagaatcg acggcaacgc gcccggcaaa atcacgtgcc ggtacagtgc
    16081 gaaacccttc acgccgtaac tgcgcgccat ctcgatcaga ttggcatcga ccgagcggat
    16141 gccgtgaaac gtgttcacgt agatcggaaa aaacacgccc agcgcgacga gaaacaactt
    16201 cgcctcttcc tcgataccga accacaggat cacgagcggg atcatcgcca gcgcgggaat
    16261 attgcggatc atctgcacgg tcgaatcgag tgcgatttcg acgggtttga agagacccgt
    16321 tgcaaggccg agcaccagcc cgatactgcc gccgatcgcg aagcccgaca ccgcgcgcca
    16381 cgtgctgacc ttcacgtcgg cccacatttc gccggactgg atcagcgacc acgcggcctt
    16441 cacgaccgca agcggctcgg gcagcacgcg cgtcgagaga aggccgctac gcgccgcgaa
    16501 ttcccacgcc agcagaatcg cgagcggcgc gagccacggc gcaagatgcg cgccgatgcg
    16561 gcgcaaaagt cccacaagcg cctcaggacg cgcgctggaa cccgcctgcg cgcctgcctg
    16621 tgtgcgtcgt tgcgcactca cagcggattg agccatgatg atttcctcat tgcctatcga
    16681 cggacgtcgt taatgagccg gccgcagatg ctcagctcga gctcgccgcc ttcggcagat
    16741 agtcgttgcc gacgatctcg ccgaacggac ccgacagcgg accgctggcc ttcttggttt
    16801 gatggcccgg cagcagcgga aacaccagtt cggcgaagcg atacgactct tcaagatgcg
    16861 ggtagccgga caggatgaac gtgtcgatac cgagctcggc gtattgcttc atcagcgcgg
    16921 ccacttgctc gggattaccg accagcgccg tgcccgcgcc tccgcgcacg agacccacgc
    16981 ccgcccacag atgcggatag acctcgagtt ccgcgcggcc gccgcgcttg ccgccatgca
    17041 aggcagacat gcgccgctgc ccttccgaat ccatcttcga gaatgacgct tgcgcacggg
    17101 caacggtttc gtcgtcgagc ttgctgatca gcttgtcggc ggcggcccat gcttcctctt
    17161 cggtttcgcg cacgatgacg tgaaagcgaa tgccgaactt gatcttgcgg ccccgcgctt
    17221 cagcacgggc gcgaatgtcg gcgatcttct tcgcgacggc tgcgggcggc tcgccccacg
    17281 tcagatacgt gtcgatgtga tcggccgcaa tattgtgtgc cgccgccgac gaaccgccga
    17341 accacagcgg cggatgcggg ttctgcaccg gcggatacag cgacttgccg cccttcgagg
    17401 tcagatgctt gccgttgaat tcgattgcat cgttggtgtg cgcggcgctc agcagcttgc
    17461 gccagatgtg caggaattca tcagtgattt cataacgcgt gtcgtggtcg acgaatatac
    17521 cgtcgccttc cagttcggcc ggaccaccac cggtcaccac attgatcagc agacgcccgt
    17581 tcgacagacg atcgaacgtg gccgccatgc gcgccgccag acccggcgac gaaaggcccg
    17641 gacggattgc caccagaaac ttcaggcgtt tggtcgcggc gatcaggctc gatgcgacga
    17701 cccaggcgtc ttcgcacgaa cggccggtcg gcagcaacac gccctcgtag ccgagcgtat
    17761 cggcggcgac ggcgacctgc tggaagtagt cgaaatcggc tgcgcgtgcg ccgtgggacg
    17821 taccgagata gcggctgtcg ccgtgagtcg gaatgaacca gaacacattc atttgctgct
    17881 cctgcttgtt agaaactgga ttgaatggag acgtgtgtaa cgacggtgcg tcgagtgaga
    17941 gcgcacacgc tggccgcacg ccgctggtcg cgcactgcta accgcgcgtc gctagtcgcg
    18001 caccgctagt cgcgcacatc catccggcac gcagccggag atgcaagcgc gaaaaagacg
    18061 gaaatacggc gatgaggcgt ggactagttc cgacagcgca tccgcgaacg cgggtgcgcg
    18121 atggagcggc tgaagatcgg catcaggcag gacatcgcga cggcttccct cgaccaaacg
    18181 tgcgcgtgat gcgctcgatt aaatgggcga cggcggcaaa cgccgtctgc atggagaaac
    18241 agtctatgga tcggggcacg gcttttgaac gatttttttt agcttaggtt ttccactttc
    18301 gtgattagcc cgacgctaaa gcatacgggt gttgcacctt cctcacgctg gaa
//
LOCUS       DATASH010000403         2040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1051095,
            whole genome shotgun sequence.
ACCESSION   DATASH010000403 DATASH010000000
VERSION     DATASH010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1051095"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            386..1360
                     /locus_tag="VHV99_11285"
     CDS             386..1360
                     /locus_tag="VHV99_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEX3636053.1"
                     /translation="MSDTRERVVVTEVGMRDGLQSIARTMPTDFKRRWIDAAYAAGVR
                     HMEVASFVPAKLLPQMADADEVIAHALTYDDLIVTALVPNLKGAQRALESGVHRIVAP
                     ISVSTAHSLANVRKTPVEMIESFAAMRELIDSAAGANARRVELIAGLSTVFGCTLQGA
                     VPYADIVQIVRAALQAGADVIALGDTTGEATPRQVGEIIERVREVAGSKLRSLHFHDT
                     RGLGLANTLIALQHGIREFDASLAGLGGCPHAPGATGNVNTEDLVFMLDSMGYDTGID
                     LTSLIASRDVLADALPGEPLYGYLARAGLPKQFIAPARRFEPSESRVL"
     gene            1360..>2040
                     /locus_tag="VHV99_11290"
     CDS             1360..>2040
                     /locus_tag="VHV99_11290"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX3636054.1"
                     /translation="MTQASSAFASSVSSAPASPVAQPGALPLAGIRVVELSHMVMGPT
                     CGMILGDLGAEVIKVEPPRGDGTRRLLGTGAGFFRTFNRNKKSVALDLDSEAGLAALH
                     KLVDTADVFVENFKPGRMTSLGLDYASLRERNPRLIYVSHKGFLNGPYEHRLALDEVV
                     QMMAGLAYMTGPVGRPLRAGSSVNDIMGGMFGAIGVLAALHERHTSGRGKEVQSALFE
                     NCVLLSAQH"
BASE COUNT          353 a          642 c          635 g          410 t
ORIGIN      
        1 gcgaccgcca tgcgctgacg ctgacggcgt cccatctgct cgatcatctg ttgcgatcgg
       61 tttgatctgt cgccgcggtt tgcctttgat gcgcaattca aacgccgttt gaaagcctgc
      121 tcaacggccg tccagcgttt cccacggtcc ccgactattt ccgaccgctc tcacaaacgt
      181 ccttcgatct ccctgtgaaa agcccttaag ggtttcccct ttgcgacgcc gaacgcccgt
      241 cccgcatgcc tctgccgcca tcgcgagcgg agatggagtg cgtcgtcaac gcagactttg
      301 ccgtgcggtc gcgggcgggc acgctgtcgc tcagctgctt cattccttgc actccacgca
      361 agattcaaga ccgggattca agaccatgag tgacacccga gagcgcgtcg tcgtgaccga
      421 ggtcggcatg cgagacggcc tgcaaagcat cgctcgcacg atgcccaccg acttcaagcg
      481 gcgctggatc gacgctgcgt atgccgccgg cgtgcggcat atggaagtcg cgtcgttcgt
      541 gccggcgaag ttgctgccgc aaatggccga cgccgatgaa gtgatcgccc acgcactcac
      601 ctatgacgat ctgatcgtga cggcgttggt gccgaacctg aagggcgcgc aacgtgcgct
      661 cgaatccggt gtgcatcgga tcgtcgcacc gatttcggtg agcacggcgc atagccttgc
      721 caatgttcgc aagacgccgg tcgagatgat cgagtcgttt gccgcgatgc gcgaactgat
      781 cgatagcgcg gctggcgcga atgcgcgacg cgtggaattg atcgccggat tgtcgacggt
      841 gttcggctgc acgctgcaag gcgcggtgcc ttacgccgat atcgtccaga tcgtgcgcgc
      901 cgcgttgcag gcgggcgccg acgtcatcgc gctcggcgac accaccggtg aagcgacccc
      961 gcgccaggtc ggcgaaatca tcgaacgggt gcgagaggtc gcgggaagca agctgcgttc
     1021 gctgcatttc cacgacacgc gcggcctcgg tctcgcgaac acgctgatag cgctgcagca
     1081 tggcatccgc gaattcgacg cgtcgctggc gggcctcggc ggctgtccgc atgcgccggg
     1141 tgcgactggc aacgtgaaca ccgaagacct cgtgttcatg ctcgacagca tggggtacga
     1201 caccggcatc gacctcacca gcctcatcgc gtcgcgcgac gtgctcgccg acgcgctgcc
     1261 gggcgaaccg ctgtacggtt atttggcgcg agccggtttg ccgaagcagt ttattgcccc
     1321 tgctcgacgt tttgaacctt ctgaatcacg ggtgctgtaa tgactcaagc ttcctctgcg
     1381 tttgcctctt ctgtttcttc tgcacctgct tctccggtgg cacaaccggg cgcgttgccg
     1441 cttgccggca tccgcgtggt cgagttgtcg catatggtga tggggccgac ctgcgggatg
     1501 attcttggcg acctcggcgc cgaagtcatc aaggtcgagc cgccgcgcgg cgacggtacg
     1561 cgccgtttac tcggcaccgg tgcgggtttt tttcgcacct tcaatcgcaa caagaagagc
     1621 gtcgcactcg atctcgatag cgaagcggga ctggccgcgc tgcacaagct ggtcgatacc
     1681 gccgatgtat tcgtcgagaa cttcaagccg ggccgcatga cgagcctggg tctcgattac
     1741 gcttcgctgc gcgaacgcaa tcccaggctg atctacgtct cgcataaggg ctttctgaac
     1801 ggtccgtatg aacaccgtct cgctctggac gaagtcgtgc agatgatggc cggcctcgcc
     1861 tacatgaccg gaccggtagg ccgtccgttg cgtgcgggaa gttcggtcaa cgacatcatg
     1921 ggtggcatgt tcggcgcgat cggcgtgctt gccgcattgc acgaacggca tacgagcgga
     1981 cgcggcaagg aagtgcaaag cgcgctgttc gaaaactgcg tgctgctttc cgcgcaacac
//
LOCUS       DATASH010000404         4800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2633446,
            whole genome shotgun sequence.
ACCESSION   DATASH010000404 DATASH010000000
VERSION     DATASH010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4800
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2633446"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="VHV99_11295"
     CDS             <1..408
                     /locus_tag="VHV99_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008924072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HEX3636055.1"
                     /translation="SVDSLLDIPGYTPEMVARLRPFVTVLPTATAVNMNTASAEVIAA
                     IVPGMNQSSAQAFVARRQTVFFHNVGDVQLALTGAGVQSISIDPNEMDVNTNYFLIHG
                     HVQHERAEVDRTTLVYRDALTHTTRIVRVQDQL"
     gene            437..1858
                     /gene="gspL"
                     /locus_tag="VHV99_11300"
     CDS             437..1858
                     /gene="gspL"
                     /locus_tag="VHV99_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HEX3636056.1"
                     /translation="MSTLIVLLPPRDPAVPSQEWQLPELPFVLLDKSGRTQRAGRSAL
                     ALLPRASSTVLMVAARDLLMMPAKLPPLRGPRLRQALPNIVEDQLIQDPQTCHIAVDP
                     QPVAGGRQLLAIIDRGWFRFFCEAFAAAGHRSVRAVPVTRCLPQAVALDTPAEVAEMV
                     NAGEPLTAGSASVATSLPGVAPVVAPGVASVVPMVAAVLGAVVQTAPALLLEGAIESA
                     VVNGAPRVELAIARGVQGEGLAVPANAVNATLSALAGAAPISLYMLTEVPGSEPGLSA
                     TSPAKLAAHVHGASPLPFEQLARRALECRFDLCQFEFASQPWRLDRSTLRRLRLPVML
                     AVAALLIAIVGANVQWLMLSRQRDAISAQMTELLLNTFPKTTVVLDAPDQMSRQLQQL
                     RVAAGELSPDDFLSLADGLARSLAPVPVNGIAALDYHDRRLDVTFKPEVKLDADFAKR
                     LARNGLSGAIDSNTGKWTIRNGQ"
     gene            1855..2358
                     /locus_tag="VHV99_11305"
     CDS             1855..2358
                     /locus_tag="VHV99_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein M"
                     /protein_id="HEX3636057.1"
                     /translation="MKAELAQSWAGFWDQRTEREKTLLAWGGGVLAVVIAWSVLWAPA
                     QDGRAHLRESLPSLQRQLAQMTAQSNEARQLAAAAQGVAPTGAALKDALTASLSDHGL
                     AATQVQIIGNAVQVQMKNASFPVWTTWVDDARKQFKVQVAEAHVTALKEDGQVDLTAS
                     LQPSTAK"
     gene            2381..3160
                     /locus_tag="VHV99_11310"
     CDS             2381..3160
                     /locus_tag="VHV99_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HEX3636058.1"
                     /translation="MTYWMRRLRAALPWVAVAALASAAVMLTLLPAAWITPQFAKQTR
                     GHVNLVDPAGSLWHGSATLMLAAGSDMSAATLLPGRIEWRTAFWPLFTGRVRMVMRHS
                     EAMPDPITVDASLRGATVTPGAMAVPASLLSGLGAPFNTLDLQGDVRLAWSDWRSFKQ
                     EAFGQMTVTLNDVSSRISLVKPLGSYRLLFQAQGTSSTLDLTTLKGPLMLTGNGNVSS
                     ASTSFHGTASAAPEARDNLAGLLNLLGRPSGPDTVTLTFVH"
     gene            complement(3317..>4800)
                     /locus_tag="VHV99_11315"
     CDS             complement(3317..>4800)
                     /locus_tag="VHV99_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEX3636059.1"
                     /translation="LLAGCAVGPDYKRPAAEIPASYKEAAPGWKVAQPADQQDRGDWW
                     TLYEDPQLNTLEDKLNASNQTIAQFAAAYRQARALVGEARAAYFPTVGASIGATRSGN
                     GSSSNGISTTASSRSGINNSFNVQLDASWEPDLWGSVTRSVNAQKAGQQGAAADLANA
                     RLSAQATLAQTYFSLRALDSTQKLLDDTVAAYQRSLQLTQNQYAAGVAARSDVIQAQT
                     QLQSAQAAAIDNGVQRAQDEHAIAVLVGEPASTFSIPPMPLTATPPAVPAQMPSALLE
                     RRPDIASAERKAAAANEQIGVAIAAFFPSLTLSATGGFENSVFSQLLTAPSRFWTLGP
                     ELAATIFDAGLRQAQTEAARAVYDQNVATYRQTILAAFQDVEDNLASQRILAQEIVVQ
                     QQAVDSARQALAIVTNEYKAGTVGFVNVLTAQTTAFTAEQKLESIAGERMVSSVGLVK
                     ALGGGWDAAQMNRETGDVAAPAPLPAATPVAQSGATPRPAQSN"
BASE COUNT          749 a         1555 c         1638 g          858 t
ORIGIN      
        1 agcgtcgatt cgctgctcga catccccggc tatacgccgg aaatggtcgc gcgcttgcga
       61 ccgttcgtca ccgtgctgcc gacagcgacg gcggtcaaca tgaatacggc ctccgccgaa
      121 gtgatcgcgg cgatcgtgcc ggggatgaat cagtcttcgg cgcaggcctt cgtcgcgcgc
      181 cggcagacgg tgtttttcca taacgtcggc gacgtgcagc tcgccttgac cggcgccggc
      241 gtacagtcga tctcgatcga cccgaatgaa atggacgtca acacgaacta ctttctgatc
      301 cacggccatg tacagcacga acgtgcggaa gtagatcgca cgaccctcgt ctatcgcgat
      361 gcattgactc acactacgcg tatcgtgcgg gtacaagacc aactatgaat aacgcctttc
      421 ccagagagag tggcctttga gcacgctgat cgttctattg ccgccgcgtg atccggcggt
      481 gccatcgcag gaatggcaac tgccggagct gccgttcgtg ctgctcgaca agtcgggccg
      541 cacccaacgc gcgggccgct cggcgctcgc gctgctgccg cgcgcttcgt cgacggtgct
      601 gatggtcgcc gcccgcgacc tgctgatgat gcccgccaag ctgccgccct tgcgcggccc
      661 gcggttgcgt caggcgttgc cgaatatcgt tgaggatcag ctgatccagg acccgcaaac
      721 ctgccacatc gccgtcgatc cgcaaccggt cgcgggcggc cggcagttgc tcgcgatcat
      781 tgaccgcggc tggttccgct tcttctgcga agcctttgcc gccgccggcc accgcagcgt
      841 gcgcgcggtg ccggtcacac gctgtctgcc gcaagccgtc gcactcgaca cacccgccga
      901 agtcgcggaa atggtcaacg caggcgagcc gctgacggcg ggctcggcga gcgtggcgac
      961 gtcgctgccc ggcgtggcgc cagtggtggc gccaggcgtg gcgtcggtgg tgccaatggt
     1021 ggcggccgtg ctcggcgcgg tcgtccagac cgcgcccgcg ttgctgctcg aaggcgcgat
     1081 cgaaagcgcg gtggtgaacg gtgcgccgcg tgtcgaactg gcgatcgcgc gtggcgtaca
     1141 aggcgaaggg ctggcggtgc cggccaacgc cgtgaacgcc acgctcagcg cgctcgccgg
     1201 cgccgcgccg atctcgctgt acatgttgac tgaagtgccc ggcagcgaac cgggcctgtc
     1261 cgcgaccagc ccggcgaagc tcgcggccca cgtgcacggg gccagtccgt tgccgttcga
     1321 gcagctcgcg cgtcgcgcgt tggagtgccg cttcgatttg tgccagttcg agttcgcctc
     1381 gcagccatgg cggctcgacc gttcgacgct gcggcggctg cgcctgccgg tcatgctggc
     1441 ggtcgccgcg ctcctgatcg cgatcgtcgg cgcgaacgtg cagtggctga tgctttcgcg
     1501 ccaacgtgac gcgatcagcg cgcagatgac cgaattgctc ctcaacacct tccccaagac
     1561 aacggtcgtg ctcgacgcgc ccgatcagat gtcgcgccaa ttgcagcagt tgcgggtggc
     1621 ggcgggtgag ctgtcgccgg acgattttct gtcgctggcc gatggcttgg cccgttcgtt
     1681 ggcgccggtg ccggtcaatg gcatcgccgc gctcgattat cacgaccggc ggctcgacgt
     1741 gaccttcaag cccgaagtga aactggatgc cgatttcgcc aagcgcctcg cgcgcaacgg
     1801 cctgagcggc gcgatcgaca gcaataccgg caagtggacc atcaggaacg gacaatgaaa
     1861 gctgaactcg cccaaagctg ggctggattc tgggaccagc gcaccgagcg cgaaaaaacg
     1921 ctgctggcat ggggcggcgg ggtgctggcc gtggtgatcg cgtggtcggt gctgtgggcg
     1981 ccggcgcagg atggccgcgc gcatctgcgt gaatcgctgc cctccttgca gcgtcagttg
     2041 gcgcagatga ccgcgcagtc gaatgaagcg cgccagctcg ccgcggcggc gcaaggtgtc
     2101 gcaccgaccg gcgccgcgct gaaggacgcg ctcaccgcct cgctcagcga tcacggcctt
     2161 gccgcgaccc aggtgcagat aatcggcaac gcggtccagg tccagatgaa gaatgcgtcg
     2221 ttccccgtgt ggacgacctg ggtcgacgac gcgcgcaagc agttcaaggt gcaggttgcc
     2281 gaggcgcacg tgaccgcgct caaggaagac ggccaggtgg atctgacggc gtcgttgcag
     2341 ccgtctaccg cgaaatgacc gcgccgttac gggatctcgc atgacttact ggatgcggcg
     2401 cctgcgcgcc gcgctaccct gggtggcggt cgcggcgctg gcgagcgcgg ccgtgatgct
     2461 cacgttgttg ccggccgcct ggatcacgcc gcagttcgcc aaacagactc gtggacacgt
     2521 caatctggtc gatccggctg gttcgctgtg gcacggttcg gccacgctga tgctcgccgc
     2581 cggttccgac atgagtgctg cgaccctgct gccggggcgg atcgaatggc gcacggcgtt
     2641 ctggccgctc ttcaccggcc gtgtgcgcat ggtcatgcgg catagcgaag cgatgcccga
     2701 cccgatcacg gtggatgcca gcctgcgcgg cgctaccgtc acgccgggtg cgatggcggt
     2761 accggcctcg ttgctgagcg gcctcggcgc gccgttcaac acgctcgatc tgcaaggcga
     2821 cgtgcgcctc gcatggtccg attggcgcag cttcaagcag gaagcgttcg gccagatgac
     2881 cgtgaccctg aacgatgtca gctcgcgcat ctcgctggtc aagccgctgg gttcgtaccg
     2941 gttgctgttc caggcgcagg gtacgtcgtc cacgctggat ctgacgaccc tcaaggggcc
     3001 gttgatgctg accggcaatg gcaatgtgtc gtcggcctcg acatcgtttc atggaacggc
     3061 cagcgccgcc ccggaagcac gcgacaacct cgcgggtttg ctgaacctgc tggggcggcc
     3121 aagcggaccg gacacggtca cgctgacgtt cgtgcactga ggcgcgggtc ggccatgcgt
     3181 ctcacgccgt tgaagtaggg atgcacgttc gcggcgtgcc gtacggcacg aatcagggcg
     3241 atgtgatgag ataaagaaag gcatgaccac atttcgcggc catgcctttt ttcttgtgct
     3301 ttaacgcgtc gcgcgcctag ttgctttgcg ccggtcgcgg cgtcgctccg ctttgtgcca
     3361 cgggcgtcgc cgccggcagg ggcgccggcg ccgcgacatc gcccgtttcg cggttcattt
     3421 gcgctgcatc ccagccgccg ccgagcgcct tcacgagccc caccgacgac accatccgct
     3481 ccccggcgat gctttccagc ttctgctccg ccgtgaacgc ggtggtctgc gcggtcagca
     3541 cgttgacgaa gccgaccgtg cccgccttgt actcgttcgt gacgatggcc agcgcctgac
     3601 gcgcggaatc caccgcttgc tgttgcacga cgatttcctg cgcaaggatg cgctgcgaag
     3661 cgagattgtc ctcgacgtcc tggaacgcag ccagaatcgt ttgacggtat gtcgccacat
     3721 tctgatcgta gacggcgcga gcggcttcgg tctgtgcctg acgcaagccg gcgtcgaaga
     3781 tcgtggcggc aagttccggc ccgagcgtcc agaagcgcga cggcgccgtc agcaattgcg
     3841 agaacaccga attttcgaag ccgccggtcg ccgacagcgt cagcgacggg aagaacgcgg
     3901 cgatcgcaac cccgatctgc tcgttcgccg ccgcggcctt gcgctcagcc gaagcaatat
     3961 ccggacgccg ctcgagcagc gccgacggca tctgcgcggg cacggcgggc ggcgtggcgg
     4021 tgagcggcat gggcggaatc gagaaggtcg aggcgggttc gccgaccagt acggcgattg
     4081 cgtgttcgtc ctgcgcgcgc tgcacaccat tgtcgatcgc ggccgcttgt gccgattgca
     4141 actgcgtttg cgcctgaatc acatcggagc gggccgcgac acccgctgcg tactggtttt
     4201 gcgtgagttg cagcgagcgc tgataggccg cgacggtgtc gtcgagcagc ttctgggtgg
     4261 agtcgagcgc gcgcagcgag aaataggttt gcgcgagcgt ggcctgagcg gacagccgtg
     4321 cattcgcgag atcggcggcg gcgccttgct ggccggcttt ctgcgcgttc accgagcgtg
     4381 tcaccgagcc ccacagatcc ggctcccaac tggcgtcgag ttgcacgttg aagctgttgt
     4441 tgatgccgga acggctcgac gccgtggtgg agatgccgtt ggacgatgag ccgttgccgg
     4501 agcgcgtggc gcctatcgac gcaccgacgg tcgggaaata cgccgcgcgc gcttcgccga
     4561 ccagcgcacg tgcctgacga taagctgcgg caaactgggc gatggtctgg ttcgacgcgt
     4621 tcagcttgtc ttcgagcgtg ttgagttgcg ggtcttcgta gagggtccac caatcaccgc
     4681 ggtcttgctg gtcggccggc tgggcgactt tccagccggg ggcggcttcc ttgtaggaag
     4741 cggggatttc ggctgcgggc cgcttgtagt cagggccgac tgcgcatccg gccagcaggg
//
LOCUS       DATASH010000405         3227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1842367,
            whole genome shotgun sequence.
ACCESSION   DATASH010000405 DATASH010000000
VERSION     DATASH010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1842367"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(946..2862)
                     /locus_tag="VHV99_11320"
     CDS             complement(946..2862)
                     /locus_tag="VHV99_11320"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004541285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transcriptional
                     regulator/glucokinase"
                     /protein_id="HEX3636060.1"
                     /translation="MSTGVQSKAVPGAGQHADGPRLLADIGGTNARFALEYSPGEIAS
                     VQVYPCADYPGVAEVIKKYLKDTRIGRVNHAAIAIANPVDGDQVSMTNHDWSFSIEAT
                     RRALGFDTLLVVNDFTALAMALPGLTDAQRVQVGGGARRPNSVIGLLGPGTGMGVSGL
                     IPADDRWIALGSEGGHATFAPSDEREDIVLQYARKKWSHVSFERVAAGPGIEVIYRAL
                     AGRDKKRVAANVDTIEIVKRAMDGEPLAAESVDVFCGILGTFAGNIAVTLGALGGIYI
                     GGGVVPRLGEFFARSSFRKRFEAKGRFEAYLQTVPTYVITAEYPAFLGVSAILAEQLS
                     NRAGGSSSAVFERIRQMRDALTPAERRVADLALNHPRSIINDPIVDIARKADVSQPTV
                     IRFCRSLGCQGLSDFKLKLATGLTGTIPVSHSQVHLGDTATDFGAKVLDNTVSAILQL
                     REHLNFDQVERAIDLLNGARRIEFYGLGNSNIVAQDAHYKFFRFCIPTIAYGDLYMQA
                     ASAALLGKGDVIVAVSKSGRAPELLRVLDVAMQAGAKVIAITSSNTPLAKRATVALET
                     DHIEIRESQLSMISRILHLVMIDILAVGVAIRRAVPSADVAETVAKARQGADDDATAV
                     LDWLSHGAASSARD"
     gene            complement(2840..>3227)
                     /locus_tag="VHV99_11325"
     CDS             complement(2840..>3227)
                     /locus_tag="VHV99_11325"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEX3636061.1"
                     /translation="PLVDTTQELNAHVAALNADPRFSVVPDVAILGMGEDGHTASIFA
                     DAPEWDHATTTAERFVAVHPGSAPHARVSWSLSALKEVKQLYLLIAGPRKMDVLNAAA
                     ASLQKNAISQLANDKGVRLDVYWCAK"
BASE COUNT          678 a         1070 c          897 g          582 t
ORIGIN      
        1 tccttgatgt gggtatttga ctctccttga tctggctttt tggcctttcc ttgaattgat
       61 agtggtctat tagcgtcgcc cctgtgcggg gcggcaccta cttttctttg ccggccgcaa
      121 agaaaagata ggcaaaagaa agcggctcaa accgcagcgc ataagcgggt cccccgcaca
      181 gctacggtag tggtgcatct ggaatctgtg ccctcgcaca ttcagcccta gtgaccaggc
      241 agtcatgctt cccgcctcgc actgcgtgct cgccggaagg gttcatcatc caaacgagtg
      301 gcttcgttgg gcttggcggg gccgtcggct tcgcctcgac gagatggcgc tacataggac
      361 cacgggtctt gtttgcgcgc tgcgcgcact atcggcttag cctcgccaag gcgcccagga
      421 cccaacggtt tttatgaagc gcccttcgcc gacgtgccca acactcacgg attttgaaag
      481 tgccccgcgg caatgcgccc aaacacccga ttggctttat gaagtgccct gcggcaatgc
      541 gcccaaacac cccattggct ttatgaagtg ctctgcagcg tcctcccaga acccgttggt
      601 tttatgaagt acccttcggc gcgcgcagcg ccgccggaag tatgacggct gtgtcactcg
      661 cgttgaatgt gcgagaacac ggattccaga tgcaccacta ccgtagctgc gcgaggggcc
      721 cgcttatggg ctggcggtgt gggccgcttt ctttgcttat ctttctttgc tgctgcaaag
      781 aaagtaagtg ccgccccgca caggggcaac actaatagac cactatcaat tcaaggaaag
      841 gccaaagacc cagctcaagg aaaaggccaa agacccacat caagaaaagg ccaccaccgc
      901 aggcacccag aaaaaaacaa cccgccgcga aggcccaacc cccccttaat cccgtgccga
      961 cgaagccgcc ccatgactca accagtcaag caccgcagta gcatcatcat cagccccctg
     1021 ccgcgcctta gcgaccgtct cggccacatc cgcgctaggc acagcgcgcc gaatcgccac
     1081 cccaacagca agaatatcaa tcatgaccag atgcagaatc cgcgaaatca tcgacaactg
     1141 cgactcgcga atctcaatgt gatcggtctc caaagccacc gtagcgcgct tagccaaagg
     1201 cgtattgctc gacgtaatag caatcacctt agcccccgcc tgcatggcga catccagcac
     1261 ccgcagtaac tcaggcgcac gccccgactt ggacacggca acgatcacat cacccttgcc
     1321 aagcaacgcg gcggaagccg cctgcatata cagatcgccg taggcaatag tcggaataca
     1381 aaaccggaaa aacttgtaat gcgcatcctg cgcgacaata ttcgaattgc cgagcccata
     1441 aaactcgatc cgccgcgcgc cgttcaacag atcgatcgcc cgctcgacct ggtcgaaatt
     1501 caaatgctcg cgcaactgca aaatcgccga cacggtgttg tccagcacct tcgcgccgaa
     1561 gtccgtcgcg gtatcgccca gatgcacctg gctatgactc accggaatcg tgccggtcaa
     1621 acccgtcgcc agcttcaact tgaaatccga caagccctgg cagccgagcg aacggcaaaa
     1681 gcggatcacc gtcggctgac tcacgtcggc cttgcgcgca atatccacaa tcggatcgtt
     1741 gatgatcgag cgcggatgat tcaacgcgag atcggccacg cggcgctcgg ccggcgtcag
     1801 cgcatcgcgc atctggcgaa tccgctcgaa cacggccgat gaactgccgc ccgcgcggtt
     1861 cgacaactgc tcggcgagaa tcgcggaaac ccccagaaac gccgggtatt ccgcggtaat
     1921 cacgtaggtc ggcacggtct gcaaatacgc ctcgaagcgg cccttcgcct cgaaccgttt
     1981 gcgaaacgac gaacgcgcga agaattcacc aagccgcggc acgacgccgc cgccaatata
     2041 gatgccgccc aatgcgccga gcgtcaccgc gatattgccg gcgaaggtgc cgagaatccc
     2101 gcagaacaca tcgacggatt cggccgcgag cggctcgcca tccatggcgc gcttgacgat
     2161 ctcgatcgtg tcgacgttcg ccgccactcg cttcttgtcg cggcccgcca gcgcgcggta
     2221 aatcacttcg atgccaggac ccgcggccac gcgttcgaac gagacgtgcg accacttctt
     2281 gcgcgcgtat tgcaacacga tatcttcgcg ttcatcagac ggcgcgaacg tggcgtggcc
     2341 gccttcgctg ccgagcgcga tccagcggtc gtcggccggg atcaagccgg atacgcccat
     2401 gccggtgccc gggccgagca ggccgatcac gctgttcgga cgacgcgcgc cgccgcccac
     2461 ctgcacgcgc tgggcatcgg tcaggccagg cagcgccatg gccagcgcgg taaagtcgtt
     2521 cacaacgagc agcgtgtcga agccgagcgc gcggcgcgtg gcttcgatcg aaaagctcca
     2581 gtcgtgattg gtcatgctca cctgatcgcc gtcgaccgga ttggcaatcg caatcgccgc
     2641 atgattcacg cggccgatcc tggtgtcctt caggtacttc ttgatgactt cggcaacgcc
     2701 cggatagtcg gcgcacggat agacctgcac cgaggcgatc tcgcccgggc tgtattcgag
     2761 cgcgaagcgc gcattggtgc cgccgatgtc ggcgagcagc ctcggtccgt cggcgtgctg
     2821 gcccgcgccc ggaacagcct tactttgcac accagtagac atcgagtctt actcccttgt
     2881 cgtttgccaa ctgcgagatg gcattttttt gtagcgaagc ggcggcggca ttcagcacgt
     2941 ccatctttcg cggtcccgcg atcagcaaat acaactgctt cacttctttc aaggcggaca
     3001 gcgaccagct cacgcgcgca tgcggcgcgc tgcctggatg cacggccacg aagcgctcag
     3061 ccgtggtggt cgcgtggtcc cactcaggcg catccgcgaa aatcgacgcg gtgtggccgt
     3121 cctcgcccat gccgagaatg gcgacgtccg gcaccacgct gaagcgcgga tccgcattca
     3181 gcgcggcaac gtgcgcgttc aactcttgcg tggtgtcgac cagtggc
//
LOCUS       DATASH010000406         4461 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1051220,
            whole genome shotgun sequence.
ACCESSION   DATASH010000406 DATASH010000000
VERSION     DATASH010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1051220"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..1263)
                     /locus_tag="VHV99_11330"
     CDS             complement(169..1263)
                     /locus_tag="VHV99_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX3636062.1"
                     /translation="MQEIKRGRRQAIKTIGAALAASTLPMPFINLRAQEHNFSGKTLR
                     LLTWSDDTGAAALRNIAATFTAKTGAKVIADRADGTSGMVAKVKAAGDRPTYDVITLA
                     GVGAAGLGDAGLLMKPDLDKLPNLKEVAPQYRTGANGFGVGYLLWSDGLIYNTSTVKT
                     APSSYEALWDPKYAGRLFLPPPEWAEAVDLAIIAAKMNGGSQQNIEPGFKKLMQLKDH
                     VMTLGENPNQVADLFRTGSLDIGGIYSPAFFPDQIRKPEYKMGVTYGMKEGFATQLMF
                     TVIPKSHPADSDLIHAFINHSLDAGVQGRMAADVLNGPVNSKAVIPAESRAFVPSPQQ
                     IAEKAILHDDKALAVVQPAWIKRYTEIFAA"
     gene            1742..2635
                     /locus_tag="VHV99_11335"
     CDS             1742..2635
                     /locus_tag="VHV99_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX3636063.1"
                     /translation="MRRLPPLNALQIFETVARHRSFTRAADHLCLTQGAVSRQIIALE
                     DYYKFPLFKRHAKGLTLTAEGELLLPAVKESFARIEEISLRLTRQRTDLALKVPTCVM
                     RWMLPKIMRFQAEYPDLHVQITTTWQHDVDFQLEPFDAAIIYASSPGVDVQAVPLFEE
                     RLTPVCAPDLLKDKPLDEIADLARHTLLHPTRDHRDWKMWLAKVTEVDAANAQAIDAE
                     HGPSFDTLDMATNAALQGFGVAISDLALIDEDVTAKRLVRPFDTVLKTGWRYYFVYPD
                     SVAHQQKLNLFRDWIAEHWEE"
     gene            complement(2742..3824)
                     /locus_tag="VHV99_11340"
     CDS             complement(2742..3824)
                     /locus_tag="VHV99_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ionic transporter y4hA"
                     /protein_id="HEX3636064.1"
                     /translation="MTTTATVLPRWTIAAPFVAWIVLGVAYALPGNALLLALIGVALC
                     AAVFTAVHHAEVVAHRVGEPFGTLVLAVAVTVIEVALIVSVMLTSGPEKADLARDTVF
                     AAVMIVCNGIVGVCLLVGGIRHREQDFQSRGAAAALAVLASLSVLTLVMPNYTTTSVG
                     PILSSSQLAFAGVSSLVLYGVFVFVQTVRHRDYFLADVPDEDVHAAPPTSGVALAAGA
                     LLLVCLVAVVLLAKVLSPVVETAVKNAGAPPAVVGIIIAALVLLPEGLAAVRAARADR
                     LQNSMNLALGSALASIGLTIPTVAGVFLWTGQPLVLGIDGKETVLLMLTLVVATLTLS
                     SGRTTILQGAVHLSLFAAYLFLSFAP"
     gene            complement(4248..>4461)
                     /locus_tag="VHV99_11345"
     CDS             complement(4248..>4461)
                     /locus_tag="VHV99_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX3636065.1"
                     /translation="SALGRKVRTALSKTKGLSVANITVRARGGAVTLAGTVPEQPQID
                     LAPQAAQGVAGVTSVKNALSIRPVGQ"
BASE COUNT          853 a         1387 c         1411 g          810 t
ORIGIN      
        1 cggcgtcgga aggagaaccg cgcacgccgg acggggcgcc ggacgctagt cccgatgccg
       61 catctgccgc cgcgtcggtc gacgttttag cagccgtgtc ctgagcagtt tccgacgcca
      121 cctccgacgc cgctgaggca cgccgcgaaa agcgcgcaag caccgcggtc acgccgcgaa
      181 aatttcggtg tagcgcttga tccatgccgg ctgcacgacg gcgagcgcct tgtcgtcatg
      241 caggatcgct ttctcggcga tctgctgcgg gctcggcacg aacgcgcggc tctcggccgg
      301 aatcaccgct ttcgaattga ccgggccgtt cagcacgtcg gcagccatgc ggccttgcac
      361 gccggcatcg agcgaatggt tgatgaaggc atggatcaga tcgctgtcgg ccgggtgcga
      421 tttcgggatc accgtgaaca tcagttgcgt ggcgaagcct tccttcatgc cgtaggtcac
      481 gcccattttg tattcgggct tgcggatctg gtccgggaaa aaggccggcg aatagatgcc
      541 gccaatatcg agcgaacccg tgcggaacag atcggccacc tggttcggat tctcgccgag
      601 cgtcatcacg tggtctttca actgcatcag cttcttgaaa ccgggttcga tgttctgttg
      661 cgagccgccg ttcattttcg cggcaatgat cgcgaggtcc accgcttcag cccattcggg
      721 cggcggcagg aacaggcgtc cggcgtattt cgggtcccat agcgcttcgt acgaagacgg
      781 cgccgtcttg accgtcgagg tgttgtagat caaaccgtcc gaccacagca gatagccgac
      841 gccgaagcca ttcgcgcccg tgcggtattg cggcgcgacc tccttcaggt tcggcagctt
      901 gtcgagatcg ggcttcatca gcagacccgc gtcgccgaga cccgcggcgc cgacgccggc
      961 cagcgtgatg acgtcgtagg taggccgatc gcccgcggcc ttgaccttgg cgaccatgcc
     1021 ggaagtgccg tcggcgcggt cggcgatcac cttcgcgccg gtcttcgccg tgaaggtggc
     1081 ggcgatgttg cgcaaggcgg cagcgccggt gtcgtcggac caggtcagga ggcgcagtgt
     1141 cttgccggaa aagttgtgct cctgcgcgcg cagattgatg aacggcatcg gcaacgtgga
     1201 cgccgcgagt gccgcgccga tcgtcttgat ggcctgcctt ctaccccgtt tgatctcttg
     1261 catggcgctc tccagtgttg aacccaacct aacgctacgc ccgaagcgtt gcgtgttgat
     1321 gcactctagg tttgtcaaaa tcctccgcca aacgaaatat gcgcatggag cgcatgaagg
     1381 aatctcatgg gctggctgtc gcgggatgct agcggcggct ttgctgcaat ccgtgggaac
     1441 gccgaggtgg tggcgtatcg gggttaacgt ccatgatcat tttgctcggg gcggcgtggt
     1501 ggaggtatga ggaaagctcg gttctggtgc ggtgtccggc gggtcctgcc cacagtcggt
     1561 gcaaatgaac gcactcaggc gcaatcgatg gcgagggcat gagcgaaatg catgcgtatg
     1621 gcttcgcacg caccgcgtgc aggtatttct gatcgcgtat gctgtggact cagtgggcgt
     1681 gatcgcgcgc aacatcgcac gcccacgcaa tgacttttcc tgatcccaga ccggacactt
     1741 catgcgacgt ctcccgccgc tcaatgcgct acagattttc gaaaccgtcg cgcggcatcg
     1801 cagcttcacg cgcgccgccg accacctatg cctgacgcaa ggcgcggtga gccggcaaat
     1861 catcgcgctc gaagactatt acaagtttcc gctctttaaa cgccacgcga aaggtctgac
     1921 gctgaccgcc gaaggcgaat tgctgctgcc ggccgtgaag gagagtttcg cgcgcatcga
     1981 agagatatcg ctgcgcctca cgcgtcaacg caccgatctc gcactgaagg tgccgacctg
     2041 cgtgatgcgc tggatgttgc cgaagatcat gcgcttccag gccgagtatc ccgatctgca
     2101 cgtgcagatc accacgacct ggcagcacga cgtcgatttc cagctcgagc cgttcgatgc
     2161 ggcgatcatc tacgcctcgt cgccgggtgt ggacgtgcag gcggtgccgt tattcgagga
     2221 gcgtctgacg ccggtgtgcg cgcccgatct gctgaaagac aaaccgctgg acgagattgc
     2281 ggatctggcg cgtcacacgc tgctgcatcc cacacgcgat catcgcgact ggaagatgtg
     2341 gctcgccaag gtgaccgagg tggatgccgc gaatgcacag gcgatcgacg ctgaacacgg
     2401 accgagcttc gacacactcg acatggccac caatgccgcg ttgcagggct tcggtgtcgc
     2461 catcagcgat cttgccttga tcgacgaaga tgtcaccgcc aagcggctcg tgcggccgtt
     2521 cgatacggtg ctcaagaccg gttggcgcta ttacttcgtg tatccggatt cagtcgcgca
     2581 tcagcagaag ctgaacctgt ttcgcgactg gatcgcggaa cattgggaag agtaggcgag
     2641 gcgcacgtta ttcctgcctc gccttagggg ccgctcactc aggccgatga acgctgccta
     2701 ggaaaccttg agcttacggc gaaggcacgt ctcccatctc gtcagggcgc gaaagacaga
     2761 aacagatacg ccgcgaacag cgacagatgc acagcgcctt gcagaattgt ggtgcgcccg
     2821 ctgctgagcg taagcgttgc gaccaccagc gtgagcatga gcagcacggt ttccttgccg
     2881 tcgataccga gcaccagcgg ctggcccgtc cacaaaaaca ccccggcgac ggttggaatc
     2941 gtcagcccga tactggcgag cgccgagccg agtgcgagat tcatactgtt ttgcaagcga
     3001 tcggcgcgcg ccgcgcgcac ggcggcgagt ccttcgggca gcagcaccag tgcggcgatg
     3061 ataatgccga ccacggccgg cggcgcgccc gcgttcttga ccgcggtttc caccaccggc
     3121 gacagcacct tcgccagcaa cacgacggcg acaaggcaca ccagcagcaa ggcgccggcc
     3181 gccagcgcga cgccgctggt gggcggcgcg gcatgcacgt cttcatcggg cacgtcggcg
     3241 agaaaataat cgcgatgccg cacggtctgc acgaacacaa acacgccata cagcaccagc
     3301 gacgagaccc ccgcgaacgc caattgcgac gaggacagaa tcggaccgac gctagtggtg
     3361 gtgtaattcg gcatgacgag cgtcagcacg gataaggagg cgagcaccgc tagcgcggcg
     3421 gccgcgccac ggctctgaaa gtcctgctcg cgatgccgga tgccgcccac cagcaggcaa
     3481 acgcccacga tgccgttgca gacgatcatc accgcggcga acaccgtgtc gcgcgcgaga
     3541 tcggctttct cggggccgga cgtcaacatc accgagacga tcagcgcgac ctcgatcacg
     3601 gttacggcca ctgccaggac gagcgtgccg aacggctcgc cgacgcgatg cgcgaccact
     3661 tccgcatggt gcaccgccgt gaacacggct gcgcacagtg cgacaccgat cagtgcgagc
     3721 agcagcgcgt tgcccggcag ggcatacgcc acccccagca cgatccaggc gacgaagggc
     3781 gcagcgatgg tccagcgggg caaaacggtc gcggttgtgg tcattcaggg cttcctttta
     3841 ttgcttgttc aggttgcttg catggactgg cgcgacgcgc ggggacgaac gcaagcgaag
     3901 cttcgagcga gagcgaatgc taatccatcc tatcaacaat gcgtcctaac gcattaaatt
     3961 ataaaggata gttcactgcg gacccacgtt tggtcagtat gcggcacagt gtgtgccgga
     4021 tgcagtggtc gagccgacaa ggcaagcgcc tgttgttttc tgcgtctgag ggaccgagtc
     4081 gcgcggatta gcgtagacga tgcggcgcgt ccaggcgtga ctcaccatcg gtcgagaatg
     4141 ctggggctgg ccactttaga gaaatcaacc ggaatgttcc agtcaggacg catcgcctgt
     4201 tagccaggcg gtgcgtccac cgtaaagcgc taccggctaa acgcggttta ctgtccgacc
     4261 ggacggatcg acagtgcgtt cttgacggat gtcacgcccg ccacgccctg cgccgcctga
     4321 ggtgccaggt cgatttgcgg ctgctcgggc accgtgccgg ccagtgtcac tgcgccgcca
     4381 cgcgcacgca cggtgatgtt ggccacgctc aggcccttgg tctttgacaa tgcggtgcgc
     4441 accttgcgtc ccaacgcact g
//
LOCUS       DATASH010000407         3539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4415184,
            whole genome shotgun sequence.
ACCESSION   DATASH010000407 DATASH010000000
VERSION     DATASH010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4415184"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..1394
                     /locus_tag="VHV99_11350"
     CDS             90..1394
                     /locus_tag="VHV99_11350"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX3636066.1"
                     /translation="MCIIGGGIAGLSTALGLAERGVADVAVLEAEQVGFGASGRNGGF
                     VFGGYSLDCADLLKTLGPVRARELYTLTTDAVDLMRKRIRRYRIDCDVTDAGVILANW
                     FDEPARLDTQRRLMRDSFGVDWEPLSATALASQLKTSRYHGGLFERNAFHFHPLKYVL
                     GVAGAAAHAGVQIHEKSPVVRLEREGARRNGARRDCAQRDSTPREGAQRDGARREGAG
                     FVVHTPHGVLEARHVVMAGGGYARNVYARVERAVLPIATYAMATEPLGARLKEALDTR
                     AAVYDTRFAFDYYRPLPDTRILWGGRISVRDRAPEVIARLLRRDLLKVYPQLHDVRIE
                     HAWGGLMSYARHKMPQIGRSTDGVWYAVGFGGHGMAPTTVSGELLAAAISGERPVPDA
                     FAAFGLTPAYGALGLAAAQLTYTAMQTRDALAARRPSTRPAP"
     gene            1514..2209
                     /locus_tag="VHV99_11355"
     CDS             1514..2209
                     /locus_tag="VHV99_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX3636067.1"
                     /translation="MKKGSKAAEPDTNGIASEAPRTEVRRKYDPEQTKRNILDVATQE
                     FSAMGLTGARVDAIAERTNTTKRMLYYYFGSKEGLYQAVLEKVYGDIRALEQDLHISE
                     LDPIEGMRALVEFTFDYHDRQRDFVRLVTIENIHGAKYVEQVKTFKGRNVTVIHTIED
                     LLSRGIASGQFRSDVDAIDLHLMISSLCFHRVGNRHTFGTAFGRDPSHPRLRARHRAM
                     IVDAVLRFVRKED"
     gene            complement(2325..>3539)
                     /locus_tag="VHV99_11360"
     CDS             complement(2325..>3539)
                     /locus_tag="VHV99_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3636068.1"
                     /translation="MGLLFSVFSWSYVVAQIPGGLFLDRFGSKLTYFLSMTFWSLCTL
                     AQGLVHGIGALFVFRLGLGVSEAPCFPTNSRVVATWFPQSERAMATGTYTVGEYIGLA
                     FFSPFMFMLMGAFGWRSLFYVVGGVGLAFGVIWWMFYREPQDHPTVNQAELDYIEAGG
                     GLTHRKKDADSAAAPAAKVATAAPAAPAAPAAKARSGFEWRTIGRLLKHRQLTGICLG
                     QFAGNSTLVFFLTWFPTYLATERHMAWLKIGFFAIMPFIAASIGVMFGGIFSDWLLRR
                     GKSPNVARKLPIIAGLLLASTIILANYVESNVVVIAILSVAFFAQGMAALGWTLVSDI
                     APDGLLGVTGGIFNFAANLAGIVTPLVVGFIVAATGSFVGALVFIGAIALIGALSYIF
                     IVGDIKRIVLVD"
BASE COUNT          680 a         1193 c         1110 g          556 t
ORIGIN      
        1 tttacgaagc caccgtcgcg cgctccgccg gacactcgtc cgcttatgcc tcaccttacg
       61 caccgctcac cggccggcgc agcgtcaacg tgtgcattat cggcggcggc atcgcgggtt
      121 tgtccacggc gttgggactc gccgagcgcg gcgtcgccga cgtcgccgtg ctcgaagccg
      181 agcaggtagg cttcggcgcc tccggccgca acggcggctt cgtgttcggc ggctacagcc
      241 tcgattgcgc ggatctgctg aaaaccctcg gcccggttcg cgcgcgcgaa ctctatacgc
      301 tgacaaccga cgccgtcgat ctgatgcgta agcgtatccg tcgctaccgt atcgattgcg
      361 acgtgaccga cgccggcgtg atcctcgcca actggttcga cgaaccggcg aggctcgaca
      421 cgcagcggcg cttgatgcgc gattcgttcg gcgtcgattg ggaaccgctg tcggccacgg
      481 cgctcgcttc acaactgaag acgagccgtt atcacggcgg tctgttcgag cgcaacgcgt
      541 tccactttca tccgctgaaa tatgtactcg gtgtcgccgg cgctgcggcc catgcggggg
      601 tgcagattca cgagaagtcg ccggttgtgc gcctggagcg cgagggtgca cggcgcaacg
      661 gcgcacggcg cgattgcgcc caacgcgata gcacgccgcg cgagggtgcc cagcgtgacg
      721 gtgcccggcg cgagggtgcc ggtttcgtgg tgcatacgcc gcacggcgtg ctcgaagcgc
      781 gccacgtggt gatggccggc ggcggctacg cgcgcaacgt ctacgcaagg gtcgagcgcg
      841 cggtgctgcc gatcgccacc tacgcgatgg ccaccgaacc gctcggcgca cgcctgaaag
      901 aggcgctgga cacccgcgcc gccgtctacg acacccgctt cgccttcgac tactatcgcc
      961 cgctgcccga cacccggatc ctgtggggcg gccgcatctc ggtgcgcgat cgcgcccccg
     1021 aggtgatcgc gcggctgttg cggcgcgatc tgctgaaggt ctatccgcag ttgcacgacg
     1081 tgcggatcga acacgcctgg ggcggcctga tgagctacgc gcggcacaag atgccgcaaa
     1141 tcggccgcag cacggacggc gtctggtacg cggtcggctt cggcggacac ggcatggcgc
     1201 ccactaccgt ctcgggcgaa ttgctggcgg cggccatctc cggcgagcgt ccggtgcccg
     1261 atgcattcgc ggccttcggt ctgacgcccg cctatggcgc gctcggtctc gcggccgcgc
     1321 aactcaccta caccgccatg cagacgcgcg acgcactcgc cgcccggcgc ccctcgaccc
     1381 ggccagcgcc gtgactcacg ccgtgacgct acgcgatacg cgacgcaatg cccggccgct
     1441 gatgcccgaa acccgcgcca gaggccgggt ttcgccgtgc tagaatgcgc cgtcactgcc
     1501 gctcgaatac acaatgaaaa agggaagcaa ggccgccgag cctgatacca acggcatcgc
     1561 gtccgaggct ccgcgtaccg aggtccggcg caagtacgat cccgaacaga ccaagcgcaa
     1621 catcctcgac gtcgcgaccc aggagttctc cgcgatggga ttgaccggtg cgcgcgtcga
     1681 cgcgatcgcg gaacgcacga acaccaccaa gcgcatgctg tactactact tcggcagtaa
     1741 agaagggttg taccaggccg tgctggaaaa agtgtacggc gacattcgcg cgcttgaaca
     1801 ggatctgcac atcagcgagc tcgatccgat cgagggcatg cgcgcgctgg tcgaattcac
     1861 gttcgactat cacgaccgcc agcgcgactt cgtgcggctc gtgacgatcg agaatattca
     1921 cggcgccaag tacgtcgagc aggtgaaaac cttcaaaggc cgcaacgtca ccgtcattca
     1981 tacgatcgaa gatctgctct ctcgcggtat tgcctcggga cagtttcgca gcgacgtcga
     2041 tgcgatcgac ctgcacctga tgatcagttc actgtgcttt caccgcgtcg gcaatcgtca
     2101 tacgttcggt acggcgttcg gccgcgatcc gtcgcacccg cgtctgcgcg cgcggcatcg
     2161 cgccatgatc gtcgacgccg tgctgcgttt cgtgcgcaag gaagactgaa gcgaggcggg
     2221 ctcaaggcaa gcttgagtcc gccgggtctg aagcaaaaaa gcgggacgtg gcttgggcca
     2281 tgtcccgctt ttcatttgct caagccattc gcgcgctgag gcgctcaatc caccagcacg
     2341 atccgcttga tatcgccgac gataaagatg tacgacagcg caccgatcaa cgcgatcgcg
     2401 ccgatgaaca ccagcgcacc gacaaacgag ccggttgccg ccacgatgaa acccaccacc
     2461 agcggcgtga cgatgccggc cagattggcg gcaaagttga agatgccgcc ggtcacgccg
     2521 agcaggccgt cgggcgcgat gtccgacacc agcgtccagc ccagcgccgc catcccctgg
     2581 gcgaaaaacg ccaccgagag aatcgcgatc accaccacgt tgctctcgac atagttggcg
     2641 agaatgatcg tcgacgcgag caggaggccg gcgatgatcg gcagcttgcg cgccacgttc
     2701 ggcgacttgc cgcgacgcag cagccaatcg gagaagatgc cgccgaacat cacgccgatc
     2761 gacgcggcaa tgaacggcat gatcgcgaag aagccgatct tcagccatgc catatggcgt
     2821 tcggtggcga gataggtggg gaaccaggtg aggaagaaca ccagcgtcga attgccggcg
     2881 aactggccga ggcagatccc ggtcaactga cgatgcttga gcaggcgtcc aatcgtgcgc
     2941 cattcgaagc cgctcctggc ctttgcagcc ggcgcggccg gtgcggccgg cgccgccgtt
     3001 gccacctttg cagccggcgc ggcggcagaa tcagcgtcct tcttccgatg tgtcaggcca
     3061 ccgcctgcct cgatatagtc gagctccgcc tgattgacgg tcggatgatc ctgcggctcg
     3121 cgatagaaca tccaccagat cacaccgaac gcgaggccca cgccgcccac cacgtagaac
     3181 agcgagcgcc agccgaacgc gcccatcagc atgaacatga acgggctgaa aaacgcgagg
     3241 ccgatatatt cgccgaccgt atacgtgccg gtcgccatcg cgcgttcgct ctgcggaaac
     3301 caggtggcca ccacgcggct attggtcgga aagcacggcg cttccgacac gccgaggccg
     3361 agccggaaca cgaacaatgc gccgatgcca tgcacgagcc cctgcgccag cgtgcacagc
     3421 gaccagaacg tcatcgacag gaaatacgtg agtttgctgc cgaagcggtc gagaaacagg
     3481 ccgcccggaa tctgcgccac cacgtagctc cacgagaaca cggagaacag cagccccat
//
LOCUS       DATASH010000408         6914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6391105,
            whole genome shotgun sequence.
ACCESSION   DATASH010000408 DATASH010000000
VERSION     DATASH010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6914
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6391105"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VHV99_11365"
     CDS             <1..184
                     /locus_tag="VHV99_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX3636069.1"
                     /translation="LTGFHGFHVFLGGTMLAVVLVRMIRGHFTAEHHFAFEGAAWYWH
                     FVDVVWLGLYVVVYWL"
     gene            complement(363..572)
                     /locus_tag="VHV99_11370"
     CDS             complement(363..572)
                     /locus_tag="VHV99_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017334311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HEX3636070.1"
                     /translation="MHILVPIAFVLIIASMVSALYFMMHDKGKTKRMVWSLATRVGLS
                     ISLFLFILFAHWMGWIQSTGIPYGR"
     gene            687..1403
                     /locus_tag="VHV99_11375"
     CDS             687..1403
                     /locus_tag="VHV99_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013337925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HEX3636071.1"
                     /translation="MKFRLIPALLILLVIAVTVRLGFWQRDRAHQKEALEAQITQFED
                     APAQPVSATPVQLKDIEFHRVKARGSFVADKVVYLDNRPYNDQPGFYVVMPFKLADGG
                     YVLVNRGWLPRNMNNRETIAPYATPQGEIEIEGIARADASRAFELGQGGSAAHQKIRQ
                     NLDTAAYAAETGLPLQSFVIQQLSDDGDKLVRDWPAPTSGVERNYGYMFQWWGMAAAA
                     LVFGLYAARRAAKKEHAPSV"
     gene            1533..2138
                     /locus_tag="VHV99_11380"
     CDS             1533..2138
                     /locus_tag="VHV99_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015001526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit I"
                     /protein_id="HEX3636072.1"
                     /translation="MPGQPNGQGSWQRGRWMLLLLAVICAAPIVVSYFVYYVVKPTGG
                     STSYGTLVQPQRPIPESLVVTGEDGKPLKLASLHGRWLMISVDNSACDKACVTKLYFM
                     RQIRAAQGPERERVVEVWLRTDAGNVSDVIQKAYADTNMLIADPAQVSAWLPTDTGTQ
                     LSDHIYMVDPNGNLMMRFPKNPNPSKIKGDVTKLLKWSSIG"
     gene            2161..3270
                     /locus_tag="VHV99_11385"
     CDS             2161..3270
                     /locus_tag="VHV99_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004543853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEX3636073.1"
                     /translation="MFVLELALIGLCFALLPLSYVWVKADDNKFRKLVWLTTFLTLDL
                     VMFGGFTRLTDSGLGCPDWPGCYGTSSPFIAHAAITAAHQLMPTGPVSMTKAWIEMIH
                     RYFAMAIGVLIIAQTLIAWTARIKRRPLHVSPWWPTSLLLLILVQGAFGAWTVTMKLQ
                     PIIVTTHLLLGLALLGTLGWLAARQTPLPAYEPEAARWRAAAIAGLVLLVAQIALGGW
                     VSTNYAVLACTDFPTCNGQWVPPMDFAHGFHLWRALGMTGDGEMITQDALVAIHWTHR
                     TFAIVVVAYLVWFALKLRRFESLRRPANGVLLVVLIQFITGLSNIVLQWPLPIAVAHN
                     GGAAILLLLLVMLNFRIAYSRPGRAVLPARDAAPA"
     gene            3277..4182
                     /gene="cyoE"
                     /locus_tag="VHV99_11390"
     CDS             3277..4182
                     /gene="cyoE"
                     /locus_tag="VHV99_11390"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEX3636074.1"
                     /translation="MDSTTLSQTPGSRVSQYIALTKPRVTQLAVFCAVIGMFLSTPGM
                     VPWTVLIGGTVGIWLLAGAAFAINCLMEQKIDAKMRRTSWRPSARGEITTPQILLFSA
                     VLGGLGMWTLYTFANPLTMWLTFATFVGYAVIYTLLLKPATPQNIVIGGASGAMPPAL
                     GWAAVTGHVPGDAWILVLIIFVWTPPHFWALALYRRKDYENAGLPMLPNTHGEKYTRL
                     HILLYTVILFVVTMMPFISGMSGVVYLAAAVLLGAVFLAYAWKIYREYSDDLARKTFR
                     YSIVYLSLLFAALLIDHYARVVIGA"
     gene            4188..4817
                     /locus_tag="VHV99_11395"
     CDS             4188..4817
                     /locus_tag="VHV99_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006760736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX3636075.1"
                     /translation="MLTQRFVRTARAAARVTVIACALGGALLVASCGKQPPAFTNLDI
                     TGNTQFGSDFSLPDTSGKIRTMADYKGKVVVLFFGYTHCPDVCPTTMAELSQALQQLG
                     PEDAKRVQVLFVTVDPDRDTPALLAQYAPAFNPTFVGLRPADQAQLTKITKDFRVYYA
                     KVPGKTPDSYTMDHTAASYVFDTDGKLRLFARDGQGATPWVHDFKLLLD"
     gene            5113..5925
                     /locus_tag="VHV99_11400"
     CDS             5113..5925
                     /locus_tag="VHV99_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX3636076.1"
                     /translation="MQRRNILKAFSAMVAGAAIVTSFGAHADDKIIKVGTIGGPDAQI
                     WEVVAKVAKREGLNVKVVEFNDYVQPNAALDAGDLDANSFQHQPYLDSQVKQRGYKIV
                     NAGLTYISPLGIYSKKLKSLKDLAQGAKIAVPNDPSNENRALLLLQAQGVIKLKAGAG
                     TNGNSATPLDVAGNPKKIKLVELDAAQLPRSLSDVDAAAINTNFALAAGLRPTKDAIA
                     LEDIHSPYANLIAVRTQDNDKPWVKKLVAAYQSEDVRQFIKTQFKGSVVPSF"
     gene            complement(6013..>6914)
                     /locus_tag="VHV99_11405"
     CDS             complement(6013..>6914)
                     /locus_tag="VHV99_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional helix-turn-helix transcriptional
                     regulator/GNAT family N-acetyltransferase"
                     /protein_id="HEX3636077.1"
                     /translation="QAVRHFNRFYTQHIGALHEHLAKSAFSLTEVRVLHELSLGRAQT
                     ASGLGRNLGLDSGYLSRLLTSFERRNLITRHPSDSDARQSLIAFTDEGHAAYAPLDTA
                     AIDEVSALLKGLTVLSQDQLITAMKVIERLLDGKPQHELVILRQPRAGECGWLVHRQA
                     QWFAAQHGWDHSFEGLLARIVADYSQRSDPVREMCWVADQDDTVVGSVCVVGVSTTVA
                     GVRLLWVEPDVRRLGIGTQLLSECVRFARRAGYTTLTLTTASTLEEPRRLCERAGFRL
                     ACATAARRFGKDLTIERWELELV"
BASE COUNT         1282 a         2163 c         2130 g         1339 t
ORIGIN      
        1 gctgacgggc ttccacgggt tccacgtgtt cctcggcggc acgatgctgg ctgtggtgct
       61 ggtgcgaatg atccgcggcc acttcacggc ggagcatcac tttgcattcg aaggcgctgc
      121 ctggtactgg cacttcgtgg atgtcgtgtg gcttgggctg tacgtcgtcg tgtactggct
      181 gtaacgcagt ctggcctgcg cccaccgccg gtttccggaa tgtgtgcagg ccgttggcgc
      241 atcgcccgtg atgggcgcgg cgcaccagcc gtacgcaagt tgtgcagcgc cgccctcgac
      301 cctgtcaggg cggcgttttg tttggcgaaa tgatcgccga gatgaagttt tttccgccag
      361 gttcaacgcc cgtaaggaat accggtcgac tgaatccaac ccatccagtg agcgaacagg
      421 atgaacagga acagcgagat cgacaagccc acccgcgtgg cgagcgacca gaccatccgc
      481 ttggttttgc ccttgtcatg catcatgaag tacagcgccg acaccatgct ggcgatgatc
      541 aggacgaagg cgatgggaac gagaatgtgc atggaaccaa ctgaaacccg aaagcgcaga
      601 ggcaagatgt ttattatcgc accgcgcgcc ggcgtctgcg gtgggttccg ggcgccgtcg
      661 caagtggtgc gggcggagcg tgttcgatga agttccgcct cattcccgcg ctgctgattt
      721 tgctggtgat tgcggtgacc gtgcggctcg gtttctggca gcgcgaccgc gcacatcaga
      781 aggaagcgct cgaagcgcaa attacacagt tcgaagacgc gcccgcgcag cctgtgagcg
      841 ccacgccggt ccaactgaaa gacatcgaat tccatcgcgt gaaggcgcgc ggcagtttcg
      901 tcgcggacaa ggttgtctat ctggataatc gcccgtataa cgaccagccg ggcttttacg
      961 tcgtgatgcc ttttaaactg gccgatggtg gttatgtgct agtcaatcgc ggctggttgc
     1021 cgcgcaacat gaacaatcgc gagacgatcg cgccctacgc gacgccgcaa ggcgagatcg
     1081 aaatcgaagg catcgcgcgt gccgatgcct cgcgtgcttt tgagttgggg cagggcggat
     1141 cggccgcgca tcagaagatt cgccagaacc tggacacggc ggcttacgcc gcggaaaccg
     1201 ggctgccgtt gcaatcgttc gtgatccagc aattgagcga tgacggcgac aagctggtgc
     1261 gtgactggcc ggcgccgacg agcggtgtcg aacgcaacta tggctacatg ttccagtggt
     1321 ggggcatggc ggccgcggcc ctcgtcttcg gcctgtacgc cgcgcgccgc gctgcaaaga
     1381 aagagcacgc gccgagcgtg taatgcatcg cccgcggttt ttcgtacccc gaacaggggc
     1441 ctcgaagccg cgcaattctt gaaagaggtc ctgtagtgtc gacgcaatct ccccgttcgc
     1501 cgcaagccgg caaacccgca acgccgaaag tgatgccagg gcaacccaat ggccagggat
     1561 cgtggcagcg cggccgctgg atgctgctgt tgctggcggt gatctgcgct gcgccgatcg
     1621 tcgtgtcgta tttcgtctat tacgtggtca agccgaccgg cggcagcacg agctacggca
     1681 cgctggtcca accccagcgg ccgattcccg aatcgctcgt cgtcacgggt gaagatggca
     1741 agccgctcaa gctcgcatcg ctgcacggcc gctggctgat gatctccgtc gacaacagcg
     1801 cgtgcgacaa agcctgtgtc accaagctgt acttcatgcg gcaaatccgc gcagctcagg
     1861 gcccggaacg cgaacgcgtc gtagaagtat ggctgcgcac cgatgcaggc aacgtgtccg
     1921 acgtgataca aaaggcctac gccgacacca acatgctgat cgccgacccg gcgcaggtgt
     1981 ccgcatggct gccgaccgac accggcactc agcttagcga ccacatctac atggtcgatc
     2041 ccaacggcaa tctgatgatg cgtttcccaa aaaatccaaa cccgagcaag atcaagggcg
     2101 acgtgaccaa gctgctcaaa tggtcgagca tcggctgatg tcgcaacaag ggtaagaaag
     2161 atgtttgtac tggaactcgc cctgatcggc ttgtgtttcg cgttgttgcc gttgtcctat
     2221 gtgtgggtca aggctgacga caacaaattc cgcaagctgg tgtggctcac caccttcctg
     2281 acgctggacc tggtgatgtt cggcggcttt acgcgtctca ccgattccgg tctcggctgt
     2341 cccgactggc ccggttgcta tggcacgtcg tcgccgttca tcgcgcacgc cgcgattacc
     2401 gccgcgcatc aattgatgcc caccggcccg gtcagcatga ccaaagcctg gatcgagatg
     2461 atccaccgtt acttcgcgat ggcgatcggc gtgctgatca ttgcgcagac cttgattgcg
     2521 tggacggctc gtatcaagcg ccgtccgctg catgtctcgc cgtggtggcc gacttcgctg
     2581 ctgctgctga ttctcgtgca gggcgcgttc ggcgcctgga ccgtgacgat gaagctgcag
     2641 ccgatcatcg tgacgacgca tttgctgctc ggcctcgcat tgctcggcac gctcggctgg
     2701 ctggccgcgc gccaaacgcc gctgccggct tatgagcccg aagccgcgcg ctggcgggcg
     2761 gcggcgatcg ccgggttggt gctgctggtc gcgcagatcg ccttgggcgg ttgggtcagc
     2821 accaattacg cggtgctcgc ctgcacggac ttcccgacct gcaacggcca atgggtcccg
     2881 ccgatggact tcgcccacgg cttccacctg tggcgcgccc tgggcatgac cggtgacggc
     2941 gaaatgatta cacaagacgc gctggtcgcg atccactgga cccaccgcac gttcgcgatc
     3001 gtggtggtcg cctatctggt atggttcgcg ctgaaactgc gccgtttcga gtcgctgcgg
     3061 cgtccggcaa acggcgtatt gctggtggtg ctgatccagt tcatcaccgg cctgtcgaac
     3121 atcgtcctgc aatggccgtt gcccattgcg gtcgcgcata acggcggggc cgcaatcctg
     3181 ctgcttttgc tcgttatgtt aaactttcgg atcgcttata gccgtcccgg ccgcgccgtg
     3241 ttgcctgcgc gcgatgccgc gccagcgtga ctccacatgg acagcacaac tctctcccaa
     3301 acacccggta gccgggtctc ccagtacatc gctctgacga agccgcgcgt cacgcagctc
     3361 gctgtgttct gcgcagtcat cggcatgttt ctgtcgacgc ccggcatggt gccgtggacc
     3421 gtcctgatcg gcggcaccgt cggcatctgg ctgctggccg gcgcggcgtt cgccatcaat
     3481 tgcctgatgg aacagaaaat cgacgcgaag atgcgtcgca cctcgtggcg gccctccgcg
     3541 cgcggcgaaa tcactacccc gcaaatcctg ctgttttcgg ctgtgctcgg cggccttggc
     3601 atgtggacgc tctacacgtt cgccaatccg ctgaccatgt ggctcacgtt cgccaccttc
     3661 gtcggctatg cggtcatcta cacgctgctg ctcaaaccgg ccacgccgca gaacatcgtg
     3721 atcggcgggg cgtcgggcgc gatgccaccg gcgctcggtt gggccgccgt caccggtcac
     3781 gtgccgggcg acgcgtggat tctcgtgctg attattttcg tgtggacgcc gccgcatttc
     3841 tgggcgctcg cactgtatcg ccggaaagac tacgaaaacg ccggtctgcc aatgctgcca
     3901 aatacgcacg gcgaaaagta cacgcgcctg catattctgc tgtacaccgt gatcctgttc
     3961 gtggtcacca tgatgccgtt catctccggt atgagcggtg tggtgtacct cgcggcggct
     4021 gtgttgctcg gtgcggtgtt tctcgcgtat gcgtggaaga tctatcgcga atattccgac
     4081 gacctggcgc gtaagacctt ccgttactcg atcgtttatc tgtcgctgct gttcgccgcg
     4141 ctgttgatcg accactatgc gcgcgtcgtg atcggcgcgt aacacccatg cttacacaac
     4201 gctttgtgcg cacggcgcgt gccgccgcgc gtgtcaccgt gattgcctgc gcacttgggg
     4261 gcgcgttgct ggtggccagc tgcggcaagc agccgccggc gttcacgaac ctcgacatca
     4321 ccggcaacac gcaattcggg agcgacttct cgctgcccga cacgtccggc aagattcgca
     4381 cgatggcgga ttacaagggc aaggtggtgg tgctgttctt cggctacaca cactgcccgg
     4441 acgtgtgccc gaccacgatg gccgagcttt cgcaagcgct ccagcaactc ggcccggaag
     4501 atgcgaagcg tgtgcaggtg ctgttcgtca ccgtcgatcc cgaccgcgac acgccggctt
     4561 tgctcgcgca atacgcgccg gcgttcaacc cgacattcgt cggcttgcgt ccggccgatc
     4621 aggcgcaact cacgaaaatc acgaaagact tccgcgtcta ttacgcaaaa gtgccaggca
     4681 agacgcccga tagctacacg atggaccaca cggcggcgag ctatgtgttc gacacagacg
     4741 gcaagctgcg tttgttcgcg cgcgacggcc agggcgccac gccgtgggtg cacgatttca
     4801 agctgctgct cgattgatgt attgacgttg ctgcattgat gcattgacgt tgatgcattg
     4861 acgcagccgg gcgcgcgcat ctgccgcgtg cccgtattca atttccctcg ctgtattccc
     4921 caagccggca tcggcaaatt cattcccggc gccagctgcg tcgccttggg ggcataccca
     4981 tatgcgatcg tgatatttcc catctcgccg ggcatatgag accatttgcg gtccagttgg
     5041 cggcccattc acaagatgcg cgccgccggc ttccacccga atctgacatc gatcagagaa
     5101 agcgagaatt acatgcagcg cagaaacatc ctcaaagcct tctcggcaat ggtcgccggc
     5161 gcggcgatcg tcaccagctt cggcgcccac gccgacgaca agatcatcaa ggtcggcacc
     5221 atcggcggtc cggacgcgca aatctgggaa gtcgtcgcga aagtggcgaa gcgtgaaggc
     5281 ctgaatgtga aggtggtcga attcaacgac tacgtgcagc cgaatgccgc gctcgacgca
     5341 ggcgatctgg acgccaacag cttccagcac cagccgtacc tcgacagcca ggtcaagcaa
     5401 cgtggctaca agatcgtcaa tgcaggcctg acgtacatct cgccgctcgg catctattcg
     5461 aagaaactga agtcgttgaa ggatctggcg caaggcgcga agatcgcggt gccgaacgat
     5521 ccgtcgaacg agaaccgcgc gctgctgctg ttgcaggcgc aaggcgtgat caagctgaag
     5581 gctggtgcgg gcacgaacgg caacagcgct acgccgctcg acgtcgccgg gaatccgaag
     5641 aagatcaaac tggtcgagct cgacgcagcg caactgccgc gctcgctgtc ggatgtcgac
     5701 gctgccgcga tcaacacgaa cttcgcgctg gccgctggtc tgcggccgac caaagacgcg
     5761 atcgcgctcg aggacatcca cagcccgtac gcgaacctga tcgccgtgcg cacgcaggac
     5821 aatgacaagc cgtgggtcaa gaagctggtt gcggcttatc agtcggaaga cgtgcgccag
     5881 ttcatcaaga cgcagttcaa gggttcggtg gtgccgtcgt tctaagcggc cacgtctggg
     5941 ccggccgcaa aacaaaaaac gccacggcga caaaccgtgg cgtttttttc atgcatcgcg
     6001 gcttcgctat cgctaaacca actccaactc ccatcgctcg atcgtcagat ccttgccgaa
     6061 gcgccgcgct gccgtcgcgc aggcgagcct gaagcctgct cgttcgcata gccgtcgcgg
     6121 ctcttcaagc gtgctggcag tggtgagtgt gagcgtggtg taccccgcgc gtctggcgaa
     6181 gcgcacgcat tcgctcaaca attgcgtgcc gataccaaga cgccgcacgt ccggctcgac
     6241 ccacaacagc cgtacgcccg ccaccgtcgt cgataccccg acgacgcaca ccgagccgac
     6301 caccgtgtca tcctgatcgg cgacccagca catctcgcgt accggatcgc tgcgttgcga
     6361 gtaatcggcg acgatgcgcg cgagcaagcc ctcgaacgaa tgatcccagc cgtgttgcgc
     6421 ggcgaaccac tgcgcctgtc gatgcacgag ccagccgcat tcgccggcac gtggttgccg
     6481 aaggatcacg agttcgtgct gcggcttgcc gtcgagcaac cgctcgatca ccttcattgc
     6541 ggtgatcaac tggtcctggg agagcaccgt gagacccttc agcagcgccg atacttcgtc
     6601 gatcgcggcc gtatcgagcg gcgcgtaggc cgcgtgaccc tcgtcggtga aggcgatcag
     6661 cgactggcgc gcgtccgagt cggacgggtg tcgcgtaatc aggttgcgtc gttcgaagct
     6721 ggtcagaagg cggctgagat agccactgtc gaggccgagg ttgcgcccga gccccgaggc
     6781 ggtctgcgcg cgtccgaggg acagttcgtg cagtacgcgc acttccgtca gcgaaaaggc
     6841 gctctttgcc aaatgctcgt gcagtgcgcc gatgtgctgg gtatagaagc ggttgaaatg
     6901 gcggacggcc tgag
//
LOCUS       DATASH010000409         3621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5204083,
            whole genome shotgun sequence.
ACCESSION   DATASH010000409 DATASH010000000
VERSION     DATASH010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5204083"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="VHV99_11410"
     CDS             <1..972
                     /locus_tag="VHV99_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014722396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEX3636078.1"
                     /translation="IMDRLKMPEGEAIEAGIVSRSIESAQRKVEARNFDVRKQLLEYD
                     DVSNDQRKVIYQQRNELLEANDITETIGAMRQGVIADIVHPFVPAGSIEEQWDVPELE
                     EVLRNEWQLDLAIQEMINESNSISSDEILEAVEAAADEAYESKVEQVGRESFSAFERS
                     IMLQTLDRSWREHLAALDHLRQGIHLRGYAQKNPKQEYKREAFELFAAMLDAVKLEVT
                     RVVMNVQIQSPEQLEQAAEQYEEQGNHLENVEFRHAEFAEAAAAAAAPAAAEAATAAM
                     IGDAMSHASSHGVASDVSVDSVPKVGRNDPCPCGSGKKYKQCHGKIA"
     gene            1122..2363
                     /gene="argJ"
                     /locus_tag="VHV99_11415"
     CDS             1122..2363
                     /gene="argJ"
                     /locus_tag="VHV99_11415"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017333474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEX3636079.1"
                     /translation="MAVNFPSIDPAQLHPVAGVTLGWAEANIRKPNRKDVLVISVGVG
                     ATVGGVFTQNRFCAAPVTVCRENLERVRAGGAPIRALVINTGNANAGTGEPGLVAARE
                     TCAELARLVDITPEQVLPFSTGVILEPLPVDRLKAGLPAALANRKEANWYDAAQAIMT
                     TDTLPKATSRQITIDGHTVTLTGISKGAGMIKPNMATMLGFLAFDAAVAQPVLDALVK
                     HVADRSFNCITIDGDTSTNDSFILIASGQSSLPAITSTDSPAYAALRDAVTEVAQTLA
                     QLIVRDGEGATKFMTVHVEGGSSVGECRQIAYAIGHSPLVKTAFYASDPNLGRILAAI
                     GYAGVDDLDVGKIDLYLDDVLVATAGGRNPAYREEDGQRVMKKSEIGIRVVLGRGNAQ
                     ATIWTCDLSHDYVSINADYRS"
     gene            2419..3288
                     /locus_tag="VHV99_11420"
     CDS             2419..3288
                     /locus_tag="VHV99_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3636080.1"
                     /translation="MDKLEQFLTRAEAVLVRLEAMLPPAAPQIDWSAAVAFRWRKRQG
                     RGYLQPVPAISSITLDDLQNIDRQKGLIEQNTRQFVHKQPANNVLLTGARGTGKSSLI
                     KACLNAYAKDGLRLIEVDKDDLHDLGDIVDLIAQRPERFVVFCDDLSFEDGESGYKAL
                     KVALDGSIAAQSDNVLIYATSNRRHLLPEYMSDNETYKHTSDGEIHPGELVEEKISLS
                     ERFGLWVSFYPFKQDDYLSIIGHWLRHFGCDDAEVEAARGDALVWALERGSRSGRVAW
                     QFARDWSGRKSEA"
     gene            3285..>3621
                     /locus_tag="VHV99_11425"
     CDS             3285..>3621
                     /locus_tag="VHV99_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX3636081.1"
                     /translation="MSEAQKNAAGRPVTEVAVGVLVQPDGRYLLAQRPAGKPYEGYWE
                     FPGGKLEAGESVEAALARELHEELGIDVKASHRWHTLEHDYPHAYVRLYFCKVTEWSG
                     EPHGRKGQAF"
BASE COUNT          720 a         1106 c         1151 g          644 t
ORIGIN      
        1 atcatggacc gcctgaagat gccggaaggc gaggcgatcg aggcgggcat cgtatcgcgt
       61 tcaatcgaat ccgcgcagcg caaggttgaa gcgcgcaact tcgacgttcg caagcaattg
      121 ctcgaatacg acgacgtgtc gaacgatcag cgcaaggtga tctaccagca gcgcaacgaa
      181 ttgctcgaag cgaacgacat caccgaaacc atcggcgcga tgcgccaagg cgtgatcgcc
      241 gatattgttc acccattcgt gccggccggc agcatcgaag agcagtggga cgtgcctgaa
      301 ctggaggaag tgctgcgcaa cgaatggcag ctcgacctcg cgattcagga aatgatcaac
      361 gagtcgaatt cgatcagctc ggacgaaatt ctcgaagccg ttgaagccgc tgcggacgaa
      421 gcctatgaat cgaaggtcga acaggtcggc cgcgaatcgt tcagcgcgtt cgaacgttca
      481 atcatgctgc aaacgctgga tcgcagctgg cgcgaacatc tggccgcgct cgatcatctg
      541 cgccagggta tccatctgcg cggttacgcg cagaagaatc cgaagcagga atacaagcgc
      601 gaggcattcg aactgttcgc cgcgatgctc gatgccgtaa agctcgaagt cacccgggtg
      661 gtgatgaacg tgcagatcca gtcgccggaa cagctggaac aggctgccga gcagtacgaa
      721 gagcagggca accacctcga aaacgtcgag ttccgtcacg ccgaattcgc cgaagcggcc
      781 gccgccgctg cagcgcctgc tgcggcggaa gcagccactg cggcgatgat cggcgacgca
      841 atgagccatg cctcgtcgca tggcgtcgcc tcggacgtga gcgtggacag cgtgccgaag
      901 gtcggccgta atgacccgtg cccgtgcggc agcggcaaga agtacaagca gtgccacggc
      961 aagattgcgt aatcccgaat gcggcgcgct tttgagcgcg ccgcttcgtt ttgcgtgatg
     1021 cttcgctagt gatttcgtgt tggttcggcc tacgtccgag ccatcagttt ccatttcctc
     1081 gatgccggcg cccgccggca tttcttcgac aggtcgcgac catggctgtc aatttccctt
     1141 cgatcgatcc cgctcaactc caccctgtcg ccggcgtcac gctgggctgg gcggaggcga
     1201 atatccgcaa gccgaaccgc aaggacgtgc tggtcatttc cgttggcgtt ggcgcgacgg
     1261 taggcggcgt gttcacgcag aaccgttttt gcgctgcgcc cgtcacggtg tgccgtgaga
     1321 atctggagcg cgtgcgcgcg ggaggcgcgc cgatccgtgc gctggtgatc aacaccggca
     1381 acgcgaacgc cggcacgggc gaaccgggcc tcgtcgcagc gcgcgaaacc tgcgccgaac
     1441 tcgcgcgtct cgtggacatc acgccggaac aggtgttacc gttttcgacg ggcgtgattc
     1501 tcgaaccgct gccggtcgat cggctgaagg ccggtctgcc ggccgcgctg gcgaaccgca
     1561 aggaagccaa ctggtacgac gccgctcagg cgatcatgac cacggacacg ttgccgaaag
     1621 ccacctcgcg ccagatcacg atcgacggcc acacggttac gctcaccggc atcagtaagg
     1681 gcgccggcat gatcaagccg aatatggcga ccatgctcgg cttcctcgcg ttcgacgccg
     1741 ctgttgcgca accggtgctc gacgcgctgg tcaagcacgt ggcggatcgc tcgtttaact
     1801 gcatcacgat cgacggcgat acctcgacca acgattcctt catcctgatc gcctcgggcc
     1861 aatcgagcct gccggcgatc acgtccaccg attcgcccgc ttatgctgcg ctgcgcgacg
     1921 cggtgactga agtcgcccag accctcgcac aactgatcgt gcgcgacggc gaaggcgcga
     1981 ccaaattcat gaccgtgcat gtcgaaggcg gctcgagcgt cggcgaatgc cgccagatcg
     2041 cgtatgcgat cggccattcg ccgctcgtga agacggcctt ctatgcatcg gatccgaatc
     2101 tcggccgcat tctcgcggcc atcggctatg ccggcgtgga cgatctcgac gtcggcaaga
     2161 tcgatctgta tctggacgac gtgctggttg caaccgccgg cggccgcaat ccggcctacc
     2221 gtgaggaaga cggccagcgc gtcatgaaaa agagcgagat cggcattcgc gttgtactcg
     2281 gccgcggcaa tgcgcaagcc acgatctgga cttgcgatct gtcgcacgac tacgtgagca
     2341 tcaacgccga ctatcgttcg taaccaggtt cctcattagg gttttttacg cggctttctg
     2401 gccgctcaac tcactgccat ggacaaactc gaacagtttc tgacccgggc cgaagccgtg
     2461 ctcgtccgcc tcgaagcgat gcttccgccc gctgcaccgc agatcgactg gtcggcggcg
     2521 gtcgcttttc gctggcgcaa gcgccagggg cgcggctatc tgcaaccggt tcccgctatc
     2581 tcgtcgatca cgctcgacga cctgcaaaac attgatcgcc agaaagggtt gatcgagcaa
     2641 aacacgcgtc aattcgtgca caagcagccg gccaacaacg tgctgctgac gggtgcgcgc
     2701 ggcacgggca agtcgtcgct gatcaaggcg tgcctgaatg cgtatgcgaa agacggcttg
     2761 cgcctgatcg aagtggataa agacgatttg cacgacctcg gcgacatcgt cgatctgatc
     2821 gcgcagcggc cggagcgctt cgtggtgttc tgcgacgacc tttcgttcga agacggcgag
     2881 tcgggctaca aggcgctgaa ggttgcgctg gacggctcga tcgctgccca gtcggacaat
     2941 gtgctgatct acgcaacgtc gaatcgccgc catctgctgc ctgagtacat gagcgacaac
     3001 gagacctaca agcacacgtc cgacggcgag atccatccag gcgaactggt cgaggaaaag
     3061 atctcgttgt ctgagcgctt cgggctctgg gtcagcttct atccgttcaa gcaggacgac
     3121 tacctgtcga tcatcggcca ctggctgcgg catttcggtt gcgacgacgc agaagtcgaa
     3181 gcagcacgtg gcgacgcgct ggtgtgggcg ctggaacgtg gttcgcgctc ggggcgcgtc
     3241 gcgtggcaat ttgcacgcga ctggtccggc cggaaatccg aggcatgagc gaggcacaga
     3301 agaacgcggc gggccgcccg gtgacggaag tcgccgtcgg cgtgctggtt cagccggacg
     3361 ggcgctatct gctcgcgcaa cgtccggcgg gcaagccgta tgagggctac tgggagtttc
     3421 cgggcggcaa gctggaagcg ggcgaatcgg tcgaggcggc gctcgcgcgc gagttgcacg
     3481 aggaactggg gatcgacgtc aaggcaagcc accggtggca cacgctcgaa cacgactacc
     3541 cgcatgcgta cgtgcggctt tatttttgca aggtgaccga atggagcggc gagccgcacg
     3601 gtcgcaaggg ccaggcattc g
//
LOCUS       DATASH010000410         2687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1250012,
            whole genome shotgun sequence.
ACCESSION   DATASH010000410 DATASH010000000
VERSION     DATASH010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1250012"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VHV99_11430"
     CDS             complement(<1..267)
                     /locus_tag="VHV99_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1840 domain-containing protein"
                     /protein_id="HEX3636082.1"
                     /translation="MLITFKCRACPDVMMLKNLAQYLVGIVGKRLGERGVITHDELAV
                     AISKLEAAISFDKQERAEHDGHFHEGEDGHEHHEIPPGLAQRAYP"
     gene            715..2505
                     /gene="argS"
                     /locus_tag="VHV99_11435"
     CDS             715..2505
                     /gene="argS"
                     /locus_tag="VHV99_11435"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004522947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX3636083.1"
                     /translation="MLPAHKHTLETLLADTVKQVALASQGASEAAFVSPTITLERPKV
                     AAHGDVACNVAMQLAKPLRANPRQLAQQIVDVLLAQPQALGLVEAAEVAGPGFINLRL
                     AAAAKQAVIAAVFAEKDAFGRSQRDAGKHVLIEFVSANPTGPLHVGHGRQAALGDALS
                     NVLASQGYDVHREFYYNDAGVQIHTLAVSTQARARGIAPGDQGWPASAYNGEYIADIA
                     KDYLNGATVAASDGEPVTGAGDVEDLEAIRRFAVAYLRREQDMDLQAFGVKFDQYYLE
                     SSLYKEGRVEKTVAALIAAGKTYEQEGALWLRTTDDGDDKDRVMRKSDGTYTYFVPDV
                     AYHVAKWERGFTKVINVQGSDHHGTIARVRAGLQGLGIGIPKGYPDYILHKMVTVMRN
                     GEEVKISKRAGSYVTVRDLIEWSGGATPGSEAAVDLIDEETIRRGRDAVRFFLISRKA
                     DTEFVFDIDLALKQNDENPVHYVQYAHARICSVIAESRARYNTDESTLAEVDVSPLTS
                     ERAMALLNKLAEFPDMLQHAADELAPHAVAFYLRDLAGEFHSFYNDRAERVLVDDAAE
                     RNARVALLAATRQVLANGLATIGVSAPVKM"
     gene            2567..>2687
                     /locus_tag="VHV99_11440"
     CDS             2567..>2687
                     /locus_tag="VHV99_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HEX3636084.1"
                     /translation="MQVIHTMAKPRRTTKQSQSKQTGGTFLGIVLGLIVGLAIA"
BASE COUNT          505 a          901 c          818 g          463 t
ORIGIN      
        1 cggatatgcg cgttgcgcga gtcccggcgg aatttcatga tgctcgtggc cgtcttcgcc
       61 ttcgtggaaa tggccgtcgt gctcggcacg ttcctgtttg tcgaagctga tcgccgcctc
      121 gagcttgctg atcgcaacgg caagttcgtc gtgggtaatc acgccacgtt cacccagccg
      181 cttgccgacg atgccgacga ggtactgggc aaggttcttc agcatcatca cgtccgggca
      241 ggcccgacat ttgaaagtaa tcaacatgac tgttcccttt ttcgttatgt tcgatccacg
      301 ctcgggcagc ccgaaatcgg cggccggcgc gcgttacgtt gacacgccga tcagacacgg
      361 cggccacggc ccttgttggg catctcattg aacatattag cacctgctaa aatccgtctg
      421 gacggaaaag cgcgccgcaa tgcactgcgc gcggcgtaac gcaggcggca tggccggccc
      481 ggttggccgc aactcacatg ccgccgcgcc aggccgcagc agcaagcgtc tcgtcatgtt
      541 gccgcaccgc gctaaagcgt tgcacgcaac gtgcctgatg acggcccaat gtgccccggc
      601 gcgtcccggc gggtgtatcg ccgctttcca cgcttctgac gaatcggttg cgccttgcgc
      661 gcggccggcg cctcttccgc atcaccggat tgcctcactg gactcgcaac aagcatgctg
      721 cccgcacata aacataccct cgaaacactg ctcgccgaca cggtgaaaca agtggcgctt
      781 gcgtcccagg gcgcaagtga agccgccttc gtctcgccca ccatcacgct cgagcgtccc
      841 aaggtcgccg cgcacggcga cgtcgcctgc aatgtggcca tgcaactcgc caagccgctg
      901 cgcgccaacc cgcgccagct ggcgcaacag atcgtcgacg tgctgcttgc gcagccgcaa
      961 gccttgggtc tcgtggaggc cgcagaagtg gccggccccg gcttcatcaa cctgcgcctc
     1021 gcggcggccg ccaagcaggc ggtgatcgcc gccgtcttcg ctgaaaagga tgctttcggc
     1081 cgctcgcagc gcgacgccgg caagcacgtc ctgatcgaat tcgtctcggc caaccccacc
     1141 ggtccgctgc acgtcggcca cggccgccag gccgcgctcg gcgacgcgct ctcgaacgtg
     1201 ctggcctcgc aaggctatga cgtccaccgc gagttctatt acaacgacgc cggcgtgcag
     1261 attcacacgc tcgccgtgtc cacccaggcg cgtgcccgcg gcatcgcccc gggcgaccaa
     1321 ggctggcccg cctcggcgta caacggcgag tacatcgccg atatcgcgaa agactatctg
     1381 aatggcgcca cggttgccgc gagcgacggc gagcccgtca cgggtgcggg cgacgtcgaa
     1441 gatctcgaag caatccgccg cttcgcggtg gcctacctgc gccgcgagca ggacatggac
     1501 ttgcaggcgt tcggcgtgaa gttcgaccag tactacctgg agtcgtcgtt gtacaaggaa
     1561 ggccgcgtcg agaaaaccgt cgcggcgctg atcgccgccg gcaagaccta cgaacaggaa
     1621 ggcgcgctgt ggctgcgcac caccgacgac ggcgacgaca aagaccgcgt gatgcgcaag
     1681 agcgacggca cctacacgta cttcgtgccg gacgtcgcct atcacgtcgc caagtgggag
     1741 cgcggcttca ccaaggtcat caacgttcag ggctcggacc accacggcac gatcgcgcgc
     1801 gtgcgcgccg gcctgcaagg tctcggcatc ggtattccga agggctatcc cgactacatc
     1861 ctgcacaaga tggtcacggt gatgcgcaac ggcgaggagg tgaagatttc gaagcgcgcc
     1921 ggcagttatg tgacagtgcg cgacctgatc gaatggtcgg gcggtgcgac gccgggctcg
     1981 gaagccgccg tcgatctgat cgacgaagag accattcgcc gcggccgcga tgccgtgcgc
     2041 ttcttcctga tttcgcgcaa ggccgatacg gaattcgtgt tcgacatcga cctggcgttg
     2101 aaacagaacg acgaaaatcc agtgcactac gtgcagtatg cgcatgcgcg gatctgctcg
     2161 gtcatcgccg aatccagggc gcgctacaac acggacgaaa gcacgctggc ggaggtggac
     2221 gtctcgccgc tcaccagcga acgcgccatg gccctgttga acaagctcgc cgagttcccg
     2281 gacatgctgc aacacgccgc cgacgaactc gcgccacacg cggtcgcatt ctatttacgc
     2341 gacctcgccg gggaattcca ctcgttctac aatgacagag ccgaacgcgt gctggtcgac
     2401 gacgccgccg agcgcaatgc gcgcgtcgcg ctgcttgcgg ccacgcgcca ggtgctggcc
     2461 aacggtctcg cgacgatcgg cgtctctgct cccgtcaaga tgtaagtcct ccggcgcaac
     2521 atgcatgcgg ccgtcgttat aatcgacggc cgttccagga ttctttttgc aggtgattca
     2581 tacgatggca aaaccacgcc gcacaacaaa gcaatcgcaa tcgaaacaaa ccggggggac
     2641 ttttctcggc atcgtgctgg gcctgatcgt cggtctcgcg atcgcgg
//
LOCUS       DATASH010000411         6244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5996088,
            whole genome shotgun sequence.
ACCESSION   DATASH010000411 DATASH010000000
VERSION     DATASH010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6244
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5996088"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..799
                     /locus_tag="VHV99_11445"
     CDS             2..799
                     /locus_tag="VHV99_11445"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HEX3636085.1"
                     /translation="MRFDAADAHWRVAPLPRFSAAQKDWLTRGGSLTAHLRTLGEVAV
                     RVTREAVALPWADEHAPLGLVPRAPVWVREVVLSVDGVPFVAAHSIAPLAASVGVWQA
                     TRRLRTRPLAELLYSDSSVARSSLVSRRLTTRHPLYRLAVQEIDATPPHALVARRSVF
                     ERHGAPLMVTECMLPALWAHLASSARTPSAHLASAQVPQRTHVREHGRPLEHTVSRAH
                     GAPRAEPEHQSERGARDSGHAPASNHHDEQAARESRHTPASDSKVKR"
     gene            799..1272
                     /locus_tag="VHV99_11450"
     CDS             799..1272
                     /locus_tag="VHV99_11450"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HEX3636086.1"
                     /translation="MLRGFPPVVAADTHTLILGSFPGEASLVATQYYAHPRNQFWRLL
                     GAVLDEPLPELAYEARLRRVLAHGIGVWDVLAACEREGSLDAAIRNASPNDFASLRAH
                     APRLAKVCFNGKTAGRFAPVISEAGYVTLVLPSSSPANAMLSFDQKLRLWRDILT"
     gene            1269..2192
                     /locus_tag="VHV99_11455"
     CDS             1269..2192
                     /locus_tag="VHV99_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HEX3636087.1"
                     /translation="MTKLIKRASAEAGAFRNKDADAGDNALNKQKAARNKKRALRDDD
                     LHDAPQIEAPRKPRFAPVTFSEEGGVRFLHFGTEWVQGAMRLRKPDHIELEYAQQMMA
                     WLLFIETPKRIVQLGLGAAALTKFAYRFLKRAKVEAVELNPAVVVAARTMFELPHDDA
                     RLTVHEMDAWDFVNDRANHGTIGALQIDVYDATARGPVLDSVGFYRAVRACLTDAGVV
                     TVNLFGDHPSFVRNMKRLNEAFDGRVVALPEVHDGNRIAIAFSGPALDVPFAALQARA
                     KLIEAELGLPARKWIKGLQESTGQNGNAFSL"
     gene            2628..2876
                     /locus_tag="VHV99_11460"
     CDS             2628..2876
                     /locus_tag="VHV99_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3311 domain-containing protein"
                     /protein_id="HEX3636088.1"
                     /translation="MAHDADANKASKHWLWLLLLPWIAMIWVPSYNKVEPQLFDFPFF
                     YWYQLLWVLISAVITALVYFKTKTRSTGNSGSSQGGAH"
     gene            2876..4426
                     /locus_tag="VHV99_11465"
     CDS             2876..4426
                     /locus_tag="VHV99_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEX3636089.1"
                     /translation="MNATATFVFVLFFIGVTILGFVAAHWRKGDLAHLEEWGLGGRRF
                     GTIVTWFLLGGDLYTAYTFIAVPALVFGAGATGFFALPYTILIYPFAFVVFPKLWSIA
                     KRQGYVTSADFVSARYGSRMLALAIAVTGIVATMPYIALQLVGIEVVIGALGFDTKGF
                     VGDLPLIIAFAILAAYTYTSGLRAPAMIAVVKDVLIYIVIFAAIIVIPPQLGGFGHIF
                     SVVPPAKLLLKAPDVSNLNGYSAYATLAVGSALALFLYPHSITAVLSSKSGNTIRRNM
                     AMLPAYSLVLGLLALLGFMALAAGVKDMPEFAPYFKAFGPNFAVPALFLHFFPSWFVG
                     VAFAAIGIGALVPAAIMSIAAANLYTRNIHKEFVNRNMSHEQETNVAKLVSLIVKVGA
                     VAFILGLPLTYAIQLQLLGGIWIIQTLPAIVLGLYTRVLDYRGLLIGWAVGIATGTWM
                     AISLKLAGSIFTIHLFGLAIPGYAAVWSLIVNLVVSVVVSLLVRVFGMQRAEDRTRPE
                     DYLDVVEG"
     gene            4552..5676
                     /locus_tag="VHV99_11470"
     CDS             4552..5676
                     /locus_tag="VHV99_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEX3636090.1"
                     /translation="MASADSRTVIARRSAISRMVRTFTSPYYRYRHAKVLHSLRVGLA
                     MIVSILATTGIDIPHGIWASVTLLVVIGGLQHHGNIRKKAAERAAGTLLGATIGLLLI
                     LQQNLLGSLPLTYVLMSIVAAICAWFAIGSSGYIGLLTAITMCIVAGHGDNLIDVGLW
                     RTLNVLIGIVIALAFSFALPLHATYSWRYGLAANLRECARIYTHLLEGEMISDDAQVK
                     RFLAINKRLVQLRSLMPSVAKEIDVPLAKLDEIQRLHRSVLSSLELLATGSLMRADAK
                     ARAAYARECGVEVHAVRGILLAMARGLRFGRATHFGIPAASPLVQACSDAALELPPDL
                     QGPYWLGQRFAEQVDRLRALLLETEPNWNIERHSRTLLQA"
     gene            complement(5730..6149)
                     /locus_tag="VHV99_11475"
     CDS             complement(5730..6149)
                     /locus_tag="VHV99_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3636091.1"
                     /translation="MYVQPYVFFNGRCEEALKYYTEKLGAEVLLMMRYKDAPPNPDPQ
                     NQPRPGLEEKIMHATVKLGSTNWMASDGHCDPAASFSGFSLSLTADDAESGEKYFNAL
                     ADGGSVTMPWQATFWSKGFGMVVDRFGLAWMVTLPDA"
BASE COUNT         1025 a         1987 c         2020 g         1212 t
ORIGIN      
        1 tatccgtttc gatgccgccg acgcccactg gcgcgtcgcg cccttacctc gcttcagcgc
       61 cgcccagaaa gactggttga cccgcggtgg atcgctgacc gcgcatctgc gcacgctggg
      121 cgaagtcgct gtgcgtgtga cgcgcgaagc tgtcgcgctg ccgtgggccg acgagcacgc
      181 tccgctcggc ctcgtgccgc gcgcgccggt gtgggttcgg gaagtggtgt tgtcggtgga
      241 cggtgtgccc tttgtcgcgg cgcatagcat cgcgccgctc gccgcaagcg tcggcgtgtg
      301 gcaggccacg cggcggttgc gtacgcggcc gctcgccgag ttgctgtata gcgatagcag
      361 cgtcgcgcgg tcatcgctgg tgagccgtag gttgaccacg cgtcatccgt tgtaccgatt
      421 ggcggtgcag gagatcgacg caacgccgcc gcatgcgctg gtcgcgcggc gctcggtgtt
      481 cgagcgccac ggcgcgccgt tgatggttac cgagtgcatg ctgccggcgc tgtgggcgca
      541 tttggcgtct tcggcgcgga caccgtctgc gcacctggcg tccgcgcaag tgccgcagcg
      601 tactcatgta cgggaacatg gccggccgct cgagcatacg gtgtcgcgcg cccatggtgc
      661 gccgcgtgcg gagcccgaac atcaatccga gcgaggcgcg cgtgactccg ggcacgcacc
      721 ggcatcgaac catcatgacg agcaggctgc gcgtgaatcc cggcacacgc cagcatcgga
      781 cagcaaggtg aagcgctgat gctgcgcggc tttcctcccg tcgtcgcggc ggatacgcat
      841 acgctgattc tcggcagctt tccgggcgag gcgtcgctcg tggcgacgca gtattacgcg
      901 catccgcgca atcagttctg gcggctgctc ggcgccgtgc tcgacgaacc gcttccggaa
      961 ctcgcctacg aagcgcgttt gcgccgcgtg ctcgcgcacg gcatcggcgt gtgggacgtg
     1021 ctcgccgcct gcgagcgcga aggcagtctc gacgccgcga tccgcaacgc cagccccaac
     1081 gatttcgcat cgctgcgcgc gcacgccccg cgactcgcca aagtctgttt caacggcaag
     1141 acggcgggcc gcttcgcgcc ggtgatcagc gaggccggct acgtcaccct cgtattgcct
     1201 tcgtcgagtc cggcgaatgc tatgctctcg ttcgaccaaa aattgcgtct ctggcgcgac
     1261 atccttacat gacgaaattg atcaagcgcg cctcggctga agcaggggca ttccgcaaca
     1321 aagacgctga cgcgggcgac aacgcgctga ataaacagaa agccgcacgc aacaaaaagc
     1381 gcgcgctgcg cgacgacgat ctgcacgacg cgccgcaaat cgaagcgccg cgcaagccac
     1441 gtttcgcacc ggtcacgttc tcggaagaag gcggcgtacg gttcctgcat ttcggcaccg
     1501 aatgggtgca gggcgcgatg cgtctgcgca aaccggatca catcgagctc gaatacgcgc
     1561 agcagatgat ggcctggctg ctgttcatcg aaacgcccaa acgtatcgtt cagctcggtc
     1621 tcggcgcagc cgcgctgacc aaattcgcct atcgcttcct gaagcgcgca aaggtcgagg
     1681 cggtcgagtt gaatccggct gtggtggttg cggcacgcac gatgttcgaa ctgccgcacg
     1741 acgacgcacg gctgaccgtg cacgaaatgg acgcatggga cttcgtcaac gaccgcgcca
     1801 accatggcac gatcggcgcg ttgcagatcg acgtctacga tgccaccgcg cgtggcccgg
     1861 tgctcgacag cgtcggcttc taccgtgcgg tgcgcgcatg tttgaccgac gcgggcgtgg
     1921 tgacggtgaa cctgttcggc gatcatccga gcttcgtgcg taacatgaag cgtctgaacg
     1981 aagcgttcga cggccgcgtg gtcgcgctgc cggaagtgca cgacggcaac cgcatcgcga
     2041 tcgccttctc gggcccggcg ctcgacgtgc cgttcgcggc gctgcaagcc cgcgcaaagc
     2101 tgatcgaagc ggaactcggt ctgccggcgc gcaagtggat caaggggctg caggagtcga
     2161 ccggccagaa cggcaacgcg ttctcgctct gatgcgtgac gagtgacgcg gtcgggccgg
     2221 gcaagccggc tcgactgtgg cgagcccagg actgtcgcga gatcgcgagg gccgccgccg
     2281 ttgcgcccac gctacggtcc tcgacgccac gacccagggt gcatcacaca gcctcgcgac
     2341 attgcacacg agggctcagt ggcgagcgac acataagtgg cccggcctga cacctgcggc
     2401 cacaccagca ttccaccttg cggcgacaca ccgtcccgtt cacctcaaac acccgcccgt
     2461 gggcctcaaa accacgtccc gcctcgggtc gtccctgctt gaccgcacgt tcgcggctcc
     2521 tatactcttt cggagctttc ggggcgcccg tggcctatct gcgacgggaa gatccaccac
     2581 ccgtcaaaca cgggccaaca tttcaataat agggaagagg agacgtcatg gctcacgacg
     2641 ccgacgcgaa caaagccagc aagcactggc tctggctgct gctgttgccc tggatcgcca
     2701 tgatctgggt gccgtcatat aacaaggtcg aaccgcaact gttcgatttt ccgttcttct
     2761 actggtacca gctcctgtgg gtgctgatca gcgcggtcat cacggcgctc gtgtacttca
     2821 agaccaagac ccgctcaacg ggcaactcgg gtagctcgca agggggcgca cactaatgaa
     2881 cgccaccgca acttttgtct tcgtcctgtt tttcatcggc gtcaccattc tcggttttgt
     2941 cgcggcccac tggcgcaagg gcgacctggc ccatctggaa gagtggggtc tcggcggccg
     3001 gcgcttcggc accatcgtca cgtggttcct gttgggcggc gacctgtaca ccgcctacac
     3061 tttcatcgcc gtgccggcgc tggtgttcgg tgcgggcgca accggtttct tcgcgctgcc
     3121 gtacacgatc ctgatctatc cgttcgcatt cgtcgtgttc ccgaaactgt ggagcatcgc
     3181 caaacgccag ggttacgtga cctcggccga cttcgtctcc gctcgctacg gcagccgcat
     3241 gctggcgctg gcgatcgccg tgaccggcat cgtcgcgacg atgccttaca tcgcgctgca
     3301 actggtcgga atcgaagtgg tgatcggcgc gctgggtttc gacaccaagg gcttcgtcgg
     3361 cgatctgccg ctgatcattg cgttcgcgat tctcgcggcc tacacgtaca cctcgggcct
     3421 gcgcgcaccg gccatgatcg cggtcgtcaa ggacgtgctg atctatatcg tgatctttgc
     3481 cgcgatcatc gtgattccgc cgcaactggg cggcttcggt cacatcttca gtgtggtgcc
     3541 gccggccaag ctgctgctga aggcaccgga cgtgtcgaac ctgaacggct acagcgcgta
     3601 tgcgacgctt gcggtgggtt cggcgctcgc gctgttcctg tacccgcact cgatcaccgc
     3661 ggtgctgtcg tcgaaatcgg gtaacaccat ccgccgcaac atggcgatgc tgccggctta
     3721 ctcgctggtg ctcggcctgc tcgcattgct cggcttcatg gcactggctg cgggcgtgaa
     3781 ggacatgccg gaattcgcac cgtacttcaa ggcgtttggc ccgaacttcg cggtgccggc
     3841 gttgttcctg cacttcttcc cgtcgtggtt cgtcggcgtg gcgttcgcgg cgatcggcat
     3901 cggcgcgttg gtgccggcgg ccatcatgtc gattgcggcg gcgaacctct acacgcgcaa
     3961 catccacaag gagttcgtga accgcaacat gtcgcatgag caggagacca atgtcgctaa
     4021 gctggtgtcg ctgatcgtca aggtcggcgc ggtcgcgttc attctcggcc tgccgttgac
     4081 gtacgcgatt cagctgcaac tgctgggcgg catctggatc atccagacgc tgccggccat
     4141 cgtgctgggt ctgtacacgc gcgtgctcga ctatcgcggc ctgctgatcg gctgggcggt
     4201 cggcatcgcc accgggacct ggatggcgat ttcgctgaag ctggcgggct cgatcttcac
     4261 gattcacctg ttcggtctcg cgatccccgg ctacgcggcg gtgtggtcgc tgatcgtcaa
     4321 cctggtggtg tcggtggtgg tgagcctgct ggtgcgggtg ttcggcatgc agcgcgccga
     4381 ggaccgtacc cgtccggaag actatctgga cgtggtcgaa ggctaagtca gccggtccat
     4441 ctcgcctgaa aagcaacgcc cgcggacaaa agtttgcggg cgtttgtctt tgtggggcac
     4501 tccagggcag gatagcggaa ctccctccaa aaaagcgtca ctcgtgccgc catggcttct
     4561 gccgattccc gcaccgtgat tgcccgccgt tcggctatca gccgcatggt gcgcaccttc
     4621 acgtcgccgt actaccgcta tcgccacgcg aaggtgctgc atagtctgcg tgtgggcctc
     4681 gcgatgatcg tgtcgattct cgcgaccacc ggcatcgata ttccgcacgg catctgggcg
     4741 tcggtgacgc tgctggtggt gatcggcggc ttgcagcacc acggcaatat ccgcaagaag
     4801 gccgccgagc gggcggcggg cacgctgctg ggcgcaacga tcgggcttct gctgattttg
     4861 cagcagaatc tgcttggctc gctgccgctc acctatgtgc tgatgtcgat cgtcgcggcg
     4921 atttgcgcat ggttcgcaat cggttcgtcc ggctacatcg gtctgctgac ggcgatcacg
     4981 atgtgcatcg tcgcggggca tggcgacaat ctgatcgacg tcggcctatg gcgcacgctg
     5041 aatgtgctga tcggcattgt gatcgcactg gcgttttcgt tcgcattgcc gctgcacgcc
     5101 acgtattcgt ggcgctacgg cctcgccgcc aatctgcgtg aatgcgcgcg aatttatacc
     5161 catttgctgg aaggcgagat gatcagcgac gacgcgcagg tcaaacgctt tctcgcgatc
     5221 aacaagcggc tggttcagtt gcgctcgctg atgccttcgg tggccaaaga gatcgatgtg
     5281 ccgctggcca agctcgatga gatccagcgg ctgcatcgct cggtgctcag ttcgctggaa
     5341 ttgctggcaa ccggctcgct gatgcgcgcc gatgccaagg cgcgcgctgc gtatgcgcgc
     5401 gagtgcggtg tcgaggtgca cgccgtgcgc ggcattctgc tggcgatggc gcgcggcctg
     5461 cgctttggcc gcgcgacgca cttcggcata ccggccgcgt cgccgctcgt ccaggcgtgt
     5521 agcgacgcgg cgcttgaatt gccgcccgac ttgcaagggc cgtattggct cgggcagcga
     5581 tttgccgaac aggtggatcg gctacgcgcg ctattgttgg aaactgaacc gaactggaat
     5641 atcgagcggc attcgcggac tttgctgcag gcttagggcg cggggcaagg tggcggaggc
     5701 caaggccaca gccgacgccc gcgaggcctt caggcgtccg gcagcgtcac catccacgcg
     5761 agaccgaagc ggtccaccac catcccgaag cccttgctcc agaaggtggc ttgccacggc
     5821 atcgtcaccg aaccgccgtc ggccagcgcg ttgaaatatt tctcgcctga ctcggcgtcg
     5881 tcagccgtca gcgagaggct gaagccgctg aaggaggctg cgggatcgca gtggccgtcg
     5941 gaggccatcc agttggtcga gccgagcttg accgtggcgt gcatgatctt ttcttccagg
     6001 ccggggcgcg gctggttctg cgggtcgggg ttgggcggcg catccttgta gcgcatcatc
     6061 agcaacactt cggcgccgag cttctcggtg tagtacttga gcgcttcttc acagcggccg
     6121 ttgaaaaata cgtaaggctg aacgtacatg atcgtctccc ggaatagggt tagcggcgcc
     6181 gcgttccttc ggcaaacggc aacgagggag caggcgtgca gacgattgta gtcgtccgtt
     6241 gggg
//
LOCUS       DATASH010000412         8807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4216943,
            whole genome shotgun sequence.
ACCESSION   DATASH010000412 DATASH010000000
VERSION     DATASH010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 8807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8807
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4216943"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VHV99_11480"
     CDS             complement(<1..320)
                     /locus_tag="VHV99_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEX3636092.1"
                     /translation="MTRVSTAVDGWRGGKGTGWRLGLILALAAWSMQAEAQAASLPLS
                     LDVTGKISKTNDAAHLSYHFTEAQLLALPAHSITTATTWTARSTFTGPLLADILKTVG
                     ASGSA"
     gene            619..1566
                     /locus_tag="VHV99_11485"
     CDS             619..1566
                     /locus_tag="VHV99_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEX3636093.1"
                     /translation="MNKPVLPLTYEQLDRWIESLQPALLAERFTMAIGILRGGAPLAL
                     MVSHAAGTPVAFVRYDRQTRTAAWDSTLPIPPAGSKVLLCEDIAGRGFTLTDCIAFLQ
                     QHGLDVRTLTAAVDDLSRMQPDYAIDARGYFALFPWERQAYTERYREDWQRVESGAAP
                     AMADDHEYATYAIDLDGILLPDVPLARYDEDLAAALAERDALLPFEMLPGIDMQRVRT
                     IITGRPELDRPRTEAWLARHGFGHMRLVMRTPGTHDESPAGAAAHKAAAALRGGVTHF
                     VESDPVQALLIAQQAPLLRVIWWDALTQTGMLVGARAWT"
     gene            complement(1579..3273)
                     /locus_tag="VHV99_11490"
     CDS             complement(1579..3273)
                     /locus_tag="VHV99_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013343157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HEX3636094.1"
                     /translation="MARIVPDDWKSLAATGAAARERETLALLEEALPDGYAVYHGVHW
                     TRLNQNFSVFGEADFVIVSPAGRVMIVEQKTGFLRETPKGLVKVYLQTERNVAIALAH
                     TVETLHRRFTAAFGAGTYFIEELLYCPDHIVKDAAIAGVDPARIVDATRRGRLAAVIR
                     EALPADEPRLACAPKIHHFLADELALTPDASALVGQAGTLVTRLAGGLASWARRLEFS
                     PFRLRVIGTAGSGKTQLAVQVMKDAVARGQRPLYVCFNRPLADHIARVAPPEAKVANY
                     HQLCDWIARDAGREPDFQSGDVFDQLETIFAQTPIDARWQFDVLIVDEGQDFQQPWVA
                     ALERLLRPGAAWWWLEDPLQNLYMREPVELPGWTTLKETTNYRSPRDILDYVRDVVGA
                     SVPVAAALASGSPFDGSDISLSVYDETNAVESSVEATKRAITQALSLGFRKQDIAVLS
                     FRGREGSAMTAQDHLGPHRLRSFTGKYDLFGNPEYREGDVLFESIYRFKGQAAPCVIL
                     TEVDFDTFDERIARKLFVGATRATMKLIIVASQRAAEHLHLRDGKEVRETREFKEA"
     gene            3540..4166
                     /locus_tag="VHV99_11495"
     CDS             3540..4166
                     /locus_tag="VHV99_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX3636095.1"
                     /translation="MITIWGRANSVNVQKVLWCCDELVLPYERIDAGLQFGRNNEPDY
                     LAMNPTGKIPTLVDDDFVLWESNSILRYLVLQYGESSLLYPSEPKLRASIDRWLDWSL
                     TTLQPVERPVFWTLVRTPAAERDAAKLAADFSAVTLLWRLLDHHLQGRFFLEGEKFTL
                     ADIVIGAYAKRWFGLDGVERPSLPNLERWYSRIATRSGFKKFIDFPLT"
     gene            4347..4928
                     /locus_tag="VHV99_11500"
     CDS             4347..4928
                     /locus_tag="VHV99_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX3636096.1"
                     /translation="MKTAKPAAKAATAADKATPAASAHHHEPGHVHGESQEAALTLAE
                     EYCRERGEKLTPIRRKVLELLLNSGRATKAYSLLDDMRQIHPGSAPPTVYRALDFLLS
                     AGLVHRIESINAFTVCHDLTQCQHGILVVCQQCGNVTELHQPKLRQALVAQIEDAGYR
                     LASDEIELKGLCAACQAAEAASASAATLVAATK"
     gene            complement(5037..5549)
                     /locus_tag="VHV99_11505"
     CDS             complement(5037..5549)
                     /locus_tag="VHV99_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3636097.1"
                     /translation="MDSNIAAAQIGGKQQDDTLAASDGLTGFFEQQQNMNGALRLDQA
                     TIVLVEDDRDSRESLALLLEAEGAHVVAVEDAEEGVEAALRLLPDAVVCDLDLPAMDG
                     FYLIQRLRDHEIRGDHVPSVAVALTGHTDEAYRLRSIGEGFQHFMTKPAVPEDLVTLL
                     HDAIDARAAG"
     gene            complement(5972..6748)
                     /locus_tag="VHV99_11510"
     CDS             complement(5972..6748)
                     /locus_tag="VHV99_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX3636098.1"
                     /translation="MARFPSDEKKVKSAPRHPVAVKARKLSKPASVAAGDSAHAAMPA
                     PTRRPGRPSGSARGPQQRGRLLDAALALFARQGIVDTTLGEIAREAGFTPAMMHYYFK
                     TRDQLLDVLIDERFAPLRAALGVPFQENPDDPVAAITQLAQRLVQVASDHPWFPSLWV
                     REVISDGGLLRQRMHERFGDTHQKASLASIARWQKEGRLNAGLEPSLVFVTLLGLTIL
                     PLATSKLWRNDPMRRNIGGEEIARHAVALLVQGIGPSKAG"
     gene            6824..8383
                     /locus_tag="VHV99_11515"
     CDS             6824..8383
                     /locus_tag="VHV99_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEX3636099.1"
                     /translation="MDQSTTTQPAELTKPDLRPNAQPGPQPGRQGADHPPIRLLFPAL
                     LLVMLLAALDQTIVSTALPTIVGELGGLNNLSWVVTAYLLSSTIVVPLYGKFGDLFGR
                     KIVLQTAIVVFLVGSALCGVAQNMTQLIVLRALQGLGGGGLLVVTMAAIADVIPPAER
                     GRFQGVFGGVFGLATVIGPLLGGFLVEHLSWRWIFYINLPLGIISLVVIGAVFKPHVR
                     HVKHTIDYMGAAFLAGALTCIILFTSQGGTILPWSSPQLWFTLGMGLVAIWGFIHEER
                     SAVEPIMPLELFKQRTFLLSSLIGFIIGVSLFGSVTFLPLYLQVVKGSTPTQAGMQML
                     PMMGGLFVMSMVTGRVISKIGKYRMFPIMGTCLVAIAMLLLSRLQIDTPIHVMYLYMG
                     LLGCGLGMVMQVLILAVQNTVEFKHMGVATSGATLFRSIGGSIGVAGFGAVFSNGLAA
                     RLEKLIPPDTELPQALGPAAIHQLPDALRNDYLHAFAGALHTVYLAAACLVLLAFALA
                     WLLKDHPLRKR"
BASE COUNT         1543 a         2865 c         2715 g         1684 t
ORIGIN      
        1 gcgctgccgg atgcgccgac tgtcttcagg atatccgcga gcagcggtcc tgtgaaggtg
       61 gaacgcgcgg tccaggtggt cgcggtcgta atcgaatggg ccggcagcgc gagtaattgt
      121 gcctcggtga agtgatatga caggtgcgcc gcgtcgttgg tcttgctgat cttgcccgtc
      181 acatcgagcg ataacggcaa cgacgccgcc tgcgcctcag cctgcatgct ccacgcggcg
      241 agcgcgagga tcagacccag ccgccaccct gtgcccttcc cgcccctcca gccatccacc
      301 gctgtgctca ccctggtcat ctgcctggct tgtcccatcg tttgttattt tgcgggcggc
      361 tccgccgcac tgacccaatc tgtcgattca accctgccag caaatctgcc aggtccgttt
      421 ccggccgcgg catcgttctt acggaacgcc cgctccagtg acgcgcgtca ctccgacgcg
      481 cgatctttga accgcatgca taatatcgcc aaaattcttt gtctcaaaaa cgggggcccg
      541 cggctgcact acgccgtgcg cggcgctccc tccaagcgac accctgctcc acgtgcgcaa
      601 cgcaacgcca taagagtcat gaacaagccg gttttgcccc tgacttacga acaactcgat
      661 cgctggatcg aatcgctgca gcccgcgttg ctggcggagc gtttcacgat ggcgatcggc
      721 attctgcgtg gcggcgcacc gcttgcgctg atggtgtcgc acgcggccgg cacgccggtc
      781 gccttcgtgc gttatgaccg ccagacgcgc acggccgcgt gggattcgac gctgccgatt
      841 ccgcccgcgg gctcgaaggt gctgctgtgc gaggacattg ccgggcgcgg ctttacgctg
      901 accgattgca tcgcgttcct tcagcagcat ggcctcgacg tcaggacgct gaccgcggcg
      961 gtcgacgatc tgagccgcat gcaacccgat tacgcgatcg acgcccgggg ttacttcgcg
     1021 ctgtttccgt gggagcgcca ggcttatacc gagcgctatc gtgaagactg gcaacgcgtc
     1081 gagtcgggcg cggcgccggc gatggccgac gaccacgaat acgccaccta cgcgatcgac
     1141 ctagacggca ttctgctacc cgacgtgccg ctcgcgcgct acgacgagga tctggccgcg
     1201 gcgctcgccg aacgcgatgc gctgctgccg ttcgagatgc tgcccggcat cgacatgcaa
     1261 cgcgtccgca cgatcatcac cggccggcct gaactggatc gcccgcgcac cgaagcgtgg
     1321 ctcgcacggc acggcttcgg tcatatgcgc ctcgtgatgc gcacgcccgg cacgcacgat
     1381 gaaagtcccg ccggcgccgc cgctcacaag gccgcggcgg cgttgcgcgg cggcgtcacg
     1441 cattttgtcg agagcgatcc ggtgcaggcg ctgctgatcg cgcagcaggc gccgctcctg
     1501 cgcgtgatct ggtgggacgc gctcacgcaa acggggatgc tggtgggcgc gcgggcgtgg
     1561 acgtgaggac cgggcttctt acgcctcttt gaactctctg gtttccctga cctctttacc
     1621 gtcgcgcaga tgcaaatgct ccgcggcgcg ctgggaagcg acgatgatca gcttcatggt
     1681 cgcccgcgtg gcgccgacga acagcttgcg cgcgatacgc tcgtcgaacg tatcgaagtc
     1741 gacttcggtg agaatcacgc acggcgccgc ctgccccttg aagcgataga tcgactcgaa
     1801 cagcacgtcg ccttcgcgat actccgggtt gccgaacaga tcgtacttac ccgtaaaact
     1861 gcgcaagcga tgcgggccga gatgatcctg tgccgtcatc gccgaacctt cgcggccgcg
     1921 aaacgacagc accgcaatat cctgtttgcg aaagccgagc gacaacgcct gcgtgatcgc
     1981 ccgcttggtc gcctcgacgc tgctctcgac ggcattcgtt tcgtcataga cggagagcga
     2041 aatatcggag ccgtcaaacg gactgcccga cgcaagggcc gccgcaaccg gcaccgacgc
     2101 gccaaccacg tcgcgcacat agtcgagaat gtcgcgcggg ctgcgatagt tggtggtctc
     2161 tttcagcgtg gtccagccgg gcagttcgac cggctcgcgc atatacagat tctgcagtgg
     2221 gtcttcgagc caccaccacg cggcgcccgg ccgcaacagg cgctcgaggg cggccaccca
     2281 gggctgctgg aaatcctgcc cttcgtcgac gatcagcacg tcgaattgcc agcgcgcgtc
     2341 gatcggcgtt tgcgcaaaaa tcgtttcaag ctgatcgaag acgtcgccgg actggaaatc
     2401 cggctcgcgg cccgcgtcgc gcgcgatcca gtcgcagagc tggtgatagt tcgccacctt
     2461 cgcctcgggc ggcgcgaccc gtgcgatgtg atccgcgagc ggccggttga aacacacgta
     2521 caaaggccgt tgcccgcgcg ccaccgcgtc tttcatcacc tgcaccgcca gttgcgtctt
     2581 gccggagccg gctgtgccga tcacgcgcag ccggaacggc gaaaactcga gccgccgcgc
     2641 ccagctcgcg aggccgcccg ccagacgcgt gaccagcgtg ccggcctgac cgaccagcgc
     2701 gctggcgtcg ggcgtgagcg cgagttcgtc ggcgagaaag tggtggattt tcggtgcgca
     2761 cgcaaggcgc ggttcatcgg ccggcaaggc ttcgcggatg accgcggcaa gccggccccg
     2821 acgcgtcgcg tcgacgatcc gcgcgggatc cacgccggca atcgccgcgt ccttgacgat
     2881 atggtccggg caatacagca gttcttcaat gaaataagtg cccgcgccga atgccgcggt
     2941 gaagcgccgg tgcagcgttt cgacggtgtg cgcgagcgcg atcgccacat tgcgttcagt
     3001 ctgaagatag accttgacga ggcccttcgg cgtttcgcgc aggaagccgg ttttctgttc
     3061 gacgatcatt acgcgcccgg ccgggctgac gatcacgaag tcggcctcgc cgaacacgga
     3121 aaaattctgg ttcaggcggg tccagtgcac gccgtgatag acggcgtagc cgtcgggaag
     3181 cgcttcttcc agcaacgcga gggtttcgcg ttcgcgtgcc gccgcgccgg tggcggcgag
     3241 gctcttccag tcgtcgggga caatgcgggc catgcggtgc ctcgtcgtat tcgggtgcgc
     3301 ccattgtacg caacgcccgc gcgttgccgc atgctttggc cggtgctttg gccggtgctt
     3361 tggccggccc tcgcggcgtg aatcgcaagg cgctcgggcc atgcgtcggc ccaccctgca
     3421 cgcggacgat tgcacaccgg cggcgcgcaa cccaggcgca tcgtgtatcg ttaccggctg
     3481 aacgccgcgc cgccgcgcgc ggcttgccaa ctgtcaatgt caagaaagtg atttgcagga
     3541 tgattacgat ctggggacgc gccaactcgg tcaacgtaca gaaagtcttg tggtgttgtg
     3601 acgaactggt gctgccgtac gagcgcatcg acgcgggcct gcaattcggc cgcaacaacg
     3661 agccggacta tctggccatg aatccgaccg gaaaaatccc gacgctggtc gacgacgact
     3721 tcgtgctgtg ggaatcgaac tcgatcctgc gctatctggt gctgcaatac ggcgagtcga
     3781 gcctcttgta tccgtcggag ccgaaactgc gcgccagcat cgaccgttgg ctcgactggt
     3841 cgctcactac gctgcaaccc gtcgagcgtc cggtgttctg gaccctcgtg cgcacgcccg
     3901 ccgccgagcg cgacgccgcg aagctcgccg ccgatttcag cgcggtcacg ttgctgtggc
     3961 gcctgctgga ccaccatctg caaggccgct tcttcctcga aggcgagaag ttcacgctcg
     4021 ccgatatcgt gatcggcgcg tacgcgaaac gctggttcgg gctggacggc gttgaacgtc
     4081 cgtcgctgcc gaatctggaa cgctggtatt cgcggattgc gacgcgctcg ggcttcaaga
     4141 aattcatcga tttcccgctg acgtaagtca tccggcagcc tcttgcctgg ccgcggcgca
     4201 cggcggcttg tcgggcaggc aggaacgaag ggggaggagg cagcaggcag gtaggtggcg
     4261 agcggcaagc ggcaagcggc aagcggcaag cggcaagcgg gcagcatgcc ggcccagcga
     4321 agcacgattg cctttataat cgacccatga agaccgccaa gcccgccgcg aaggccgcaa
     4381 ccgccgccga taaagccacc cccgctgcct ccgctcatca tcatgagcca gggcacgtgc
     4441 acggtgaatc gcaggaagcc gcactgactc tcgcggaaga gtattgccgc gaacgcggtg
     4501 aaaaactcac accgatccgc cgcaaagtac tcgagttgtt gctgaattcc gggcgcgcca
     4561 ccaaagccta ttcgttgctc gacgacatgc gccagatcca tcccggctcg gcgccgccca
     4621 cggtttaccg ggcgctggac tttctgttgt cggctgggct cgtgcaccgc atcgaatcga
     4681 tcaatgcgtt caccgtctgc catgacctga cgcaatgcca gcacggcatt ctggtggtct
     4741 gccagcaatg cggcaacgtc accgaactgc accagcccaa gctgcgccag gcactcgtcg
     4801 cacaaattga agacgcgggc taccgccttg ccagcgacga aatcgaattg aagggcttat
     4861 gtgccgcctg tcaggcagcg gaggcggcca gcgcgagtgc cgcaacattg gtcgccgcca
     4921 ccaaatgacc gtggcgcctg cgatcagcca ttaagctgat tgcggccggt ttcggccgcg
     4981 caagcttaac ggcccagact gcggcaaaaa gaaaccgcca ttgcccgcaa agcggctcaa
     5041 cccgccgcgc gggcgtcgat tgcgtcgtgc aacagcgtca cgaggtcttc cggcaccgcc
     5101 ggcttggtca tgaaatgctg gaagccttcg ccgatgctgc gcaagcggta ggcctcgtcg
     5161 gtatggcccg tgagcgccac cgccaccgaa ggtacgtggt caccgcgaat ctcgtgatcg
     5221 cgcagccgct ggatcagata aaagccatcc atggccggca aatcgaggtc acacaccacg
     5281 gcatcaggta acagccgcag cgccgcttcg accccctctt ccgcatcttc cactgcgacg
     5341 acatgcgccc cttccgcctc gagtaacaat gccagcgatt cccggctgtc ccggtcgtct
     5401 tcgacgagca cgatcgtggc ctgatcgagt ctcaatgctc cgttcatgtt ctgttgttgc
     5461 tcaaaaaaac cggttaggcc gtcagacgcg gccaacgtat cgtcctgctg tttgccgccg
     5521 atttgcgcgg ctgcgatgtt gctgtccatg gttccgactc gatttcatgc gtcgagttgc
     5581 atacaggccc gtgattgtaa atcatggcct gggcgcaacg cgtgcgcaca gtaacgcgcc
     5641 aaaattgcac gccgatgaaa aaccggccct cggcgccagt gtcctggcag accgatgccg
     5701 acacccgatt cgccggggcg gccggtgcaa tcccgtgcgc accgctttgt ccgcccggca
     5761 tcttctctga cattcatgtt cttttgcgac gccggcgctg tcgtgtttca cgcgcggcgt
     5821 tcgtgtccac ccctgtggta gcacgatgcg tgccgcgccg gtgaaggcgc cgcgctgccc
     5881 ggcacttatc cgccgaacgg aaagacgaag cctgagtacg agagatggcg acagaagaat
     5941 aatcgtgtgg gtcagccggt gtgacggtgt gtcagcccgc tttgctgggg ccgatgccct
     6001 gcaccagcaa agccactgca tgccgggcga tctcctcgcc gccgatattg cgccgcatgg
     6061 gatcgttgcg ccacagcttc gaagtggcca gcggcagaat cgtcagaccc agcagcgtga
     6121 cgaacaccaa agacggctcc agccccgcat tcagtcgccc ttccttctgc caacgcgcaa
     6181 tggaggccaa cgacgctttc tgatgcgtgt cgccgaaccg ttcatgcatg cgctggcgca
     6241 gcagaccgcc gtcgctgatc acttcgcgca cccacagcga tggaaaccac gggtggtcgc
     6301 tcgccacctg gaccagacgc tgcgcgagct gcgtgattgc cgccaccggg tcgtcgggat
     6361 tttcctgaaa cggcacgccg agcgccgcgc gcagcggcgc gaaacgctcg tcgatcagca
     6421 cgtcgagtag ttgatcgcgc gtcttgaagt agtaatgcat catcgccggc gtgaagcccg
     6481 cttcgcgggc aatctcgccg agcgtcgtat ccacgatacc ctgccgtgcg aataaggcga
     6541 gcgcggcgtc cagcaggcgg ccgcgttgct gcgggccgcg cgccgagccc gaaggcctgc
     6601 cgggacgccg ggtgggtgcg ggcattgcag cgtgcgcaga atcacccgcc gcgaccgacg
     6661 ccggcttcga aagcttgcgc gccttcacgg cgactgggtg gcgcggcgcg ctcttcactt
     6721 tcttttcatc tgacggaaac ctggccattg cattcgattt gacgagtgat gcgatgtcac
     6781 atatattaat ctccaagtta attaatttca acgccgtggt gccatggacc aatcgacaac
     6841 gactcaaccc gccgaactga ccaagcccga cctgcggccc aatgctcagc caggcccgca
     6901 acccggcagg cagggcgccg accatccgcc gatccgtctg ctgtttccgg cgctgctgct
     6961 ggtcatgctg ctcgccgcgc tcgatcagac cattgtctcg accgctctgc cgaccatcgt
     7021 cggcgaactg ggcgggctca acaatctgtc gtgggtggtg accgcttacc tgctcagctc
     7081 gactatcgtc gtgccgctgt acggtaaatt cggcgatctg ttcggccgca agatcgtgct
     7141 gcagacggcg atcgttgtgt tcctcgtcgg ctccgcgctg tgcggcgttg cgcagaacat
     7201 gacccagctg atcgtgctgc gcgcgctgca agggctcggc ggcggagggt tgctggtggt
     7261 gacgatggcc gcgatcgccg acgtgattcc gccggctgaa cgcggccgct ttcagggcgt
     7321 gttcggcggc gtgttcggtc tcgccacggt gatcggaccg ctgctcggcg gctttctcgt
     7381 cgagcatctg tcatggcgct ggattttcta tatcaacctg ccgctcggca tcatttcgct
     7441 ggtggtgatc ggcgcggtgt tcaaaccgca cgtgcggcat gtaaagcaca ccatcgatta
     7501 catgggcgcc gcatttctcg ccggcgcact cacctgcatc atcctgttta cgagccaggg
     7561 cggcacgatc ctgccgtggt cctcgccgca gttgtggttc acgctgggca tggggctcgt
     7621 agcgatctgg ggtttcattc acgaagaacg cagcgcggtc gaaccgatca tgccgctcga
     7681 actcttcaag cagcgcacgt ttttgctgag cagtctgatc ggcttcatca tcggcgtgtc
     7741 gctgttcggc tcggttactt tcctgccttt gtatttgcag gtggtcaaag gctccacgcc
     7801 cacgcaagcc ggcatgcaga tgttgccgat gatgggtggc ctgttcgtga tgtcgatggt
     7861 aaccggccgt gtcatcagca agatcggcaa gtaccgcatg ttcccgatca tggggacctg
     7921 cctcgtggcg atcgcgatgc tgttgctttc gcgtctgcag atcgacacgc caattcatgt
     7981 gatgtatctc tacatgggcc tgctcggctg cggtcttggc atggtgatgc aggtgctgat
     8041 tctcgccgtg cagaacaccg tcgaattcaa gcatatgggc gtggccactt cgggcgcgac
     8101 cttgttccgc tcgatcggcg gctcgatcgg cgtggcaggt tttggtgcgg tgttctcgaa
     8161 cggactcgcc gcccgcctcg aaaagctgat cccgcctgac accgaattgc cacaagcgct
     8221 gggacccgcc gcgatccatc aattgcctga cgcgttgcgc aacgattatc tgcatgcgtt
     8281 cgccggtgcg ctgcacacgg tgtatctcgc ggccgcttgc ttggtgttgc tggccttcgc
     8341 gctcgcgtgg ttgctgaaag atcatccgct tcgcaagcgc tgatcaccgt tgtgttttct
     8401 tccggcgctt gatgcaagcg tttaatgacg aggttgacga ggtcgaacgc cacgcgcttc
     8461 gacctcgttc tattttcacc gtcacgctac gcacgttgtg caaccgccgc aattcatcgc
     8521 gcatggcgcc gccccgaggc gactcgaaaa ggcgtgaaaa aagccttgcg tccgctcgtt
     8581 ctgctggcat aggcactatt tcgtcgtacc taccaatgtg cggtagcgca catagaatag
     8641 gtgcgaacgt cgattgacgt tccgcgcgaa acgccacata ttggaaactc cagcgcaagc
     8701 ttgggcttgc gtctccaaga ctctccggcg catgtagatc gtgattccgc ggacaaggct
     8761 ccagcttcaa cacgatccgg aacatgccgt aatggcatcg ttttcca
//
LOCUS       DATASH010000413         3066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5405218,
            whole genome shotgun sequence.
ACCESSION   DATASH010000413 DATASH010000000
VERSION     DATASH010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5405218"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1330)
                     /locus_tag="VHV99_11520"
     CDS             complement(<1..1330)
                     /locus_tag="VHV99_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX3636100.1"
                     /translation="MNVRATDAEAAHAGAQREVERAVASIVERARAAQRGFEAAGQDV
                     LDAAAHAAAWAIMEPARNRQLADLAVRDTGLGNADDKFRKNFRKTLGLLRDLRGQKTT
                     GVIARHDATGIVEIARAVGVVAAITPSTNPAATPVNKIVNALKCGNAVIVAPSPKGYS
                     SCALLIDFIHAQFARVGLAPGLVQMLPAPISKAATAELMRQCDLVVATGSQANVRMAY
                     ASGTPAFGVGAGNVASIVTASADLHDAAHKIARSKTFDNATSCSSENSVVIESAVYPD
                     MLAELSACGGVMLDTAQKAQLQAALWSNGKLSAHCTARSAASIARAAGLNDIAAREPA
                     FLMVEESGFGADYPFSGEKLAPVLTVYRADTFDAAADTVRGIYAYMGAGHSVGLHSSD
                     PGEAVRLGSTLPVARVIVNQAHCLATGGNFDNGLPFSLSMGCGTWGRNNFS"
     gene            1528..2292
                     /locus_tag="VHV99_11525"
     CDS             1528..2292
                     /locus_tag="VHV99_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX3636101.1"
                     /translation="MSTSNQSNAAAVRAFRVLETLAEASGPLSMTDLVHALELPKQTV
                     HRILLQLMDAWLVTRSAGDRLYECSPRVRMLAVNVLMHAGPAAARHVLLEQLVAKIGE
                     TCNLTMLAGNDVVYVDRVETEWPLRMHLQPGSHVPLHCSASGKLLLSFLPKDRRERVI
                     ETLPLRAYSERTITDRGALRKELAETRRRQLAINNQEHLQGLIAIAVPVMLNRNRACA
                     AIAVQAPVGRVVLDDLLAFLPDLRLAAEETAKTFRE"
     gene            2454..>3066
                     /locus_tag="VHV99_11530"
     CDS             2454..>3066
                     /locus_tag="VHV99_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006403862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX3636102.1"
                     /translation="MTKADTPTLRAFALLEHLVRAEGPVSLADIAQDVDLPKASLHRM
                     LASLEAGGLVIREPGQKNAYVIGPRLAQLGLGVMMHSGARRLRHAILSHLVADLGETC
                     NLTMLHETEVLYLDRIEAPWPLRLDLKPGSHVPAHCSASGKLLLAMLPREQRSALIRT
                     LKLDRFTPNTITDPELLEAELDRTAHKRIAIDNEEFVAGIACVA"
BASE COUNT          559 a          998 c          956 g          553 t
ORIGIN      
        1 tcgagaaatt gttgcggccc caggttccgc atcccatcga cagcgaaaag ggcaagccgt
       61 tatcgaagtt gccgccggtc gcgaggcaat gcgcctgatt cacgatcacg cgcgccaccg
      121 gcagcgttga accgagccgc accgcctccc ccgggtcgga ggaatgcagc ccgaccgagt
      181 gccccgctcc catgtaggcg tagatgccgc gcacggtatc ggcggcggcg tcgaaggtgt
      241 ccgcgcgata aaccgtcagc accggtgcga gcttttcgcc cgagaacggg tagtcggcgc
      301 cgaagccgct ttcctccacc atcagaaatg cgggttcgcg cgcggcaatg tcgttaagtc
      361 ccgcagcgcg tgcaatcgac gcggccgagc gcgcggtgca gtgtgccgat agcttgccgt
      421 tcgaccatag ggccgcctgc agctgcgcct tttgcgcggt gtcgagcatc acgccaccgc
      481 aagcgctcag ttcggccagc atgtctgggt acaccgcgct ctcgatgacg acgctgtttt
      541 cagatgaaca actcgtggcg ttgtcgaacg ttttcgagcg tgcaatcttg tgcgccgcat
      601 cgtgcagatc ggccgacgcg gtaacgatcg aagccacatt gcccgcgccc acgccgaacg
      661 cgggcgttcc gcttgcgtag gccatccgca cattcgcctg cgagccggtc gccacgacca
      721 gatcgcactg ccgcatcagt tcagcggtgg ccgccttgct gatcggcgcg ggcaacatct
      781 ggacgagacc cggcgcgagc ccaactctcg cgaattgcgc gtgaatgaaa tcgatcagca
      841 gcgcgcacga cgaatagccc ttgggagaag gcgccacgat gacggcgttg ccgcatttga
      901 gggcgttgac gatcttgttg accggggtgg ccgccggatt ggtggagggc gtgatcgccg
      961 ccaccacgcc gacggcccgc gcgatctcga caatcccagt ggcgtcatga cgcgcgatca
     1021 cacccgtggt cttctgcccg cgcagatcgc gcaacaggcc gagcgtcttg cgaaagttct
     1081 tgcggaactt gtcgtccgcg ttgccgagac cggtgtcgcg caccgcgagg tcggcaagct
     1141 gccggttgcg cgcgggctcc atgatcgccc acgcggcggc gtgagcggcg gcatcgagca
     1201 cgtcctgtcc cgcggcctcg aagccgcgct gcgcggcgcg cgcgcgttcg acgattgacg
     1261 caacggcgcg ttcgacttcg cgctgtgcgc ccgcgtgtgc tgcttcggcg tctgtggccc
     1321 tgacattcat acgttcactc cggactgaaa agattggcgt tgataccaat ctagcgagcc
     1381 gcatctgctt cggagtcccg ttttttgatc tattccctgc gtggacgtag cgagaatcgg
     1441 aaagtgcaaa attcgggact ttccatagtc ccgaaaacta aacagtccga tgcacaatcc
     1501 aacttataaa tacgatgagg agacagcgtg tcgacatcga accaatcgaa tgcggcggcc
     1561 gtacgcgcgt ttcgggtact cgaaacgttg gccgaggcca gcggcccgct ttccatgacg
     1621 gatctcgtcc atgcgctcga actgcccaag cagaccgtgc atcggattct gctgcagttg
     1681 atggatgcgt ggcttgtcac gcgcagcgcc ggtgaccggc tgtatgagtg ttcgccgcgc
     1741 gtgcggatgc tggcggtcaa tgtgctgatg catgccgggc cggcagccgc ccgtcatgta
     1801 ctgctcgagc aactggtggc caaaatcggc gagacctgca atctgacgat gctggccggc
     1861 aacgacgtgg tctatgtgga ccgggtggaa acggagtggc cgctgcgcat gcatcttcaa
     1921 ccgggctcgc atgtgccgct gcactgttcg gcaagcggca agctgttgct cagcttcctg
     1981 ccgaaggaca gacgcgaacg cgtcatcgaa accctgccgc tgcgcgccta ttccgagcgg
     2041 accatcacgg atcgcggcgc gttacgcaag gagttggccg agacgcggcg gcgccagctc
     2101 gcgatcaaca atcaggaaca tttgcagggg ctgatcgcga tcgcggtgcc ggtcatgctg
     2161 aatcgcaatc gcgcctgcgc ggccattgcg gtgcaggcgc cagttgggcg ggtggtgctc
     2221 gacgacctgc tggctttcct gcccgatctg cgtctcgctg cagaggaaac cgctaaaacg
     2281 ttccgggaat agtgcggacg caagcacgtc gcattgcgcc gcgcgccgca ttccgaaaaa
     2341 cgagatgccc caggcggaat ttccgccctt taagcgctgc aaacttcgcg acaaccactg
     2401 ccaacaaagg cgcacgttac aatgaagccg atccattttc cggaacggcc cacatgacta
     2461 aagcggatac ccctaccctg cgcgcgtttg ccctgctcga gcatctcgta cgcgccgaag
     2521 gtccggtgtc gcttgccgat attgcccagg acgtcgacct gccgaaggct tcactgcatc
     2581 gcatgctggc ttcgctcgag gccggtggcc tcgtgatccg cgaaccgggc cagaaaaacg
     2641 cctacgtgat cgggccgcgt ctcgcgcagc tcggtcttgg cgtcatgatg cactcgggtg
     2701 cgcgccggct gcggcacgca atcctgtcgc acctggtcgc cgatctcggc gagacctgca
     2761 atctcaccat gttgcacgaa accgaagtgc tgtacctcga ccgcatcgaa gcgccctggc
     2821 cgttgcggct cgatctcaag cccggctcgc acgtgccggc gcattgcagc gcgagcggca
     2881 agctgctgct ggccatgctg ccgcgcgaac agcgcagcgc cctcatccgc acgctgaagc
     2941 tcgaccgctt cacgccgaat accatcaccg atcccgaact gctggaagcg gaactcgacc
     3001 gcaccgcgca caagcgcatc gccatcgaca atgaggaatt tgtcgccggc atcgcgtgcg
     3061 tggccg
//
LOCUS       DATASH010000414         4483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5009426,
            whole genome shotgun sequence.
ACCESSION   DATASH010000414 DATASH010000000
VERSION     DATASH010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5009426"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(795..1070)
                     /locus_tag="VHV99_11535"
     CDS             complement(795..1070)
                     /locus_tag="VHV99_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011547500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4148 domain-containing protein"
                     /protein_id="HEX3636103.1"
                     /translation="MKSLTIAIAFIGATATVSAFAQSTTAPAQQNAMTTMQVAQNTAA
                     AVNTAGSWVPPYGQATGEKTRAEVYQDLVHSEKDGQRAYLDSTLYSR"
     gene            complement(1230..1798)
                     /locus_tag="VHV99_11540"
                     /pseudo
     CDS             complement(1230..1798)
                     /locus_tag="VHV99_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902363.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1863..2537)
                     /locus_tag="VHV99_11545"
     CDS             complement(1863..2537)
                     /locus_tag="VHV99_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006478810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3636104.1"
                     /translation="MPRILTIEDDELIAHDIVRTLSAQGCSVDVAHTGREGMAKVMAT
                     DYDVVTLDRMLPDLDGLTIVATMRGVGMETPVLVMSAMSDVDQRIQGLRAGGDDYLTK
                     PFSPEEMFARVEVLLRRRPRHAKAETLLRAGALELDLVRRKVTHGQRELDLQPTEFRV
                     LEFMMRHSGQVLTRTMIFEGVWGCRFDPGTNLIDVHIGRLRKKVERPGERPLIRTIRG
                     SGYLLD"
     gene            complement(2553..3476)
                     /locus_tag="VHV99_11550"
     CDS             complement(2553..3476)
                     /locus_tag="VHV99_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017423798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3636105.1"
                     /translation="MNQLQAMRVFTRVVDLASFNLAAKQLGMSAAAVTRSVGTLEAHL
                     NMRLLNRTTRGLSLTDVGREYLDGCRTIIEKLDEMESNLLETTRDPQGTLRIATPMTF
                     AAAGLGALLAAYRTLHPRVDFDVTTFDTPIDLVGGGFDVCFSDDRRLASSSLVSRTLT
                     SINEMTVASPAYLARHGTPRDPAALNRHGLLSVSDGVSRTWEFTDVDGIYRVSTGSAL
                     SATSSAMVRVAALNHMGIALLPQPIIADDLARGSLVPVLEQFEINGGPRQTSILYSGR
                     NYLSMRVRSFIDFAVSQYRAPDRPVALRAVA"
     gene            complement(3666..4040)
                     /locus_tag="VHV99_11555"
     CDS             complement(3666..4040)
                     /locus_tag="VHV99_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636106.1"
                     /translation="MSRPMSIEKVNEYGQNAVRIAIEDSAVLLEAADLDAVIEHLCVL
                     RATMRPEVPKEPLRTHQYVIEIDPCWHTEKHPLHDGAVLFLRHSGLGWAGFALPTESL
                     ARLHHALTQQLEASLEVHGMLN"
BASE COUNT          924 a         1301 c         1336 g          922 t
ORIGIN      
        1 actgtgcggg ggacccgctt aagagttggc ggtgtgagcc gctttctttt gcctactttt
       61 ctttgcggca ggcaaagaaa agtaggtgcc tgccccgcac aggggcgacg ctaatagacc
      121 aataacaaat caaggaaagg ccaacgccat aaaagcgcag acacccaagc acaacaccag
      181 cagaaaaaac caaaaaaatt ccacctaccg caggtacacg cacaaattcg tcatctgacg
      241 tcaatcagcc agtcgaccgg gcggccattc gacccccatc ctagcccaac cgcatcaagc
      301 caaagagcgc gctgccaaaa gcgcccccca ctcctgttca cgaacaaaaa tagatattgc
      361 aaaaactcac gggtccgaca cagttgtcgg cggtggtggt tgtcgttgtc gtcgttgcgc
      421 agccggatag cagcagtcca gctacaacag cggtagcggc gaacgaggcc attcgcttga
      481 tcgtagtgcg aaaattcatt gaagtcagca tgagaaatgt tgcatattac gtttaacgat
      541 agagccagtt cagatagttc acctgcccat cgtctcggat ttgatcagtt gccggcaaat
      601 cagggtccgt gttttgccat ggccattcgt tgcgttcggc gatggccgtt cgccaacgga
      661 tgccagcccg gtcgagcgct cagtttccgg ccaccggcgg cgcggcgacg gccgacgtgg
      721 ccgcgtttgc gagaccgcag gtgacaagca ccgcctatgc ggcgtgcacc cgatggctgt
      781 tctttacgcg catctcaacg tgagtaaagg gtcgagtcca gataggcgcg ctgaccgtcc
      841 ttctccgaat gcaccagatc ctgatagacc tccgcgcgcg tcttctctcc agtcgcctga
      901 ccgtaaggcg gcacccagga ccccgcagta ttgaccgcag cggcggtgtt ctgcgcgact
      961 tgcatcgtcg tcatcgcgtt ctgttgcgcc ggcgctgtgg tcgattgagc gaaagcggaa
     1021 accgttgcgg ttgcaccgat gaaagcgatg gcgatcgtca gagatttcat gttggtaatc
     1081 ctcacaaatc cgattggatg gaaccgatgc gcgaaaccgc aatgcattcg tggatcggtt
     1141 gataaggagg atagtcacct ggctttagcg gatcagtgat ctacgcatga aaagatattc
     1201 atccgttgga gcgtcggcga ccctttggtt cagggggccg tcgggcatgt gtcatgaaca
     1261 taaaaacgtt tcatgaatcg gccggttttg atcatgcata ggtcaccgcg caaatctaca
     1321 tttgcgcgca cccggacggt gtgcgggatc ggttcgctca tgcgcgggct ggcgctttca
     1381 gccgagcccg cagcgccgtc cggcgaccaa ccgcggccca acggcctcga acggtcccat
     1441 cgaggccagc aggcggcgcg tatcgaggtt caatgcatcg atttgcgtgg tgagttcggt
     1501 ggtctgtgcg gtcaccactt ccagatagct gactacgcca tcacgatatt gcgacagcga
     1561 cagcgacagc gacagcgaca gcgacagcga cagcgacagc gacagcgaca gcgacaacat
     1621 gcgttgcggc aacgagcgcc ttgtcctgct gcgtggcttc gtcgccgaga tgatgcaatt
     1681 gggcaaggtt gtcttcgacc tgctggaagg cgagcagcac ggcggcccgg tagttcgcgc
     1741 cgttctcggc gagcttggcg cgcgcctgct gcgtcattgc ttcacggcgg ccgccgttgt
     1801 gacaacgcta acgctgcatg cagcgctaac gctccgtcag aaaatctgcg cgtaaagcgg
     1861 cgttagtcca acagatagcc tgagccgcga atcgtgcgaa ttaatggacg ttcgccgggc
     1921 ctctcgactt tcttgcgcaa acggcctatg tgtacgtcga tcaggttggt gccgggatcg
     1981 aagcggcagc cccacacgcc ttcgaaaatc atcgtgcgcg tcaacacctg gccgctgtgg
     2041 cgcatcatga attcgagcac gcggaattcg gtgggttgca gatcgagttc gcgctgcccg
     2101 tgggtgacct tgcggcgcac cagatcgagt tccagcgcgc cggcgcgcag cagcgtttca
     2161 gctttggcgt gacgtggccg acggcgcaac agcacctcga cgcgcgcgaa catttcctcg
     2221 ggtgagaacg gcttggtcag atagtcgtcg ccgccggcgc gcaggccttg aatgcgctga
     2281 tcgacgtcgg acatcgcgct catcaccagc accggcgttt ccatgccgac accacgcatg
     2341 gtcgccacga tggtcaggcc gtcgagatcg ggcagcatgc ggtcgagtgt gacgacgtcg
     2401 tagtcggtcg ccatcacttt cgccatgcct tcgcggccgg tgtgcgccac gtcgacggag
     2461 caaccctgcg cgctgagcgt gcgcacgatg tcgtgtgcga tcaactcatc atcttcgatc
     2521 gtcaatattc tgggcatgat aagcgagcca gttcaggcca ccgccctcaa tgcaaccggg
     2581 cggtccggcg cgcgatactg gctgaccgcg aagtcgatga aactgcgcac gcgcatcgac
     2641 aggtaattgc gtccggaata gagaatcgac gtctggcgcg ggccgccgtt gatttcgaac
     2701 tgttccagca ccggcacgag cgagcctcgc gcgagatcgt cggcaatgat cggctgcggc
     2761 agcaaggcga tacccatgtg gttgagcgcg gccacgcgca ccatcgcgct gctcgtcgcg
     2821 ctcagcgcgc tgccggtgct gacgcggtag atgccgtcga cgtccgtgaa ctcccaggtg
     2881 cgcgacacac cgtcggaaac gctcagcagg ccatgacggt tcaacgccgc cggatcgcga
     2941 ggggtgccgt gacgcgccag ataggcaggc gaggcgaccg tcatttcatt gatgctggtc
     3001 agtgtgcggc ttaccagcga ggaactggcc aggcgccggt cgtctgagaa acacacgtcg
     3061 aagccgcctc ccaccagatc gatcggcgta tcgaacgttg tcacgtcgaa gtccacacgt
     3121 ggatgcagcg tgcgataggc ggccagcaat gcacctagcc cggcggccgc gaacgtcatg
     3181 ggcgtggcga tccgcagcgt accttgggga tcgcgcgtcg tctcaagcag gttcgattcc
     3241 atctcgtcga gcttctcgat gatcgtgcgg caaccgtcga gatattcgcg gcccacatcg
     3301 gtcagcgaaa gaccgcgcgt ggtgcggttc agcaagcgca tattcagatg cgcttccagt
     3361 gtgccgacgc tgcgcgtgac cgccgcggcc gacataccta actgtttcgc cgcaagattg
     3421 aagctggcga gatcgaccac tcgtgtgaag acacgcatcg cttgtagctg gttcatgttg
     3481 gaacagtaga gaaggacgat gggtccatca tcggcgatcg cggataaaac ggcgccgcca
     3541 ggcgcattaa ctctttttta agtttgtgca atcttcatga tccatgaggc gagcctgcaa
     3601 tccgacccgt gctgcgcgaa gcgcaacacg ccgcgtgcgt ttgcaattca gctgatccgg
     3661 ctgcttcagt tcagcatgcc gtgtacttcg agcgatgctt ccagttgctg tgtcaacgca
     3721 tgatgcagcc tggcgaggct ctcggtgggg agcgcgaagc ccgcccagcc cagaccggaa
     3781 tgcctcagga acagcacggc gccgtcatgc agcggatgct tttcggtgtg ccagcacgga
     3841 tcgatttcaa tcacatactg atgcgtgcgc agcggctcct tgggcacttc cgggcgcatt
     3901 gtggcgcgca acacgcagag gtgttcgatc acggcgtcca gatcggctgc ttcgagcagg
     3961 accgcactgt cctcgatggc gatgcgaacc gcgttctgtc cgtactcgtt gactttctcg
     4021 atgctcatgg ggcgcgacat ttctttgctc ctttccagct cttacggttc acaaattatg
     4081 gtgccgcctc tcttaaccca gtcttaaaga gcacgatcca gggatgaaac tttgtttaag
     4141 aaagccggca ggcatgattt atggtgcagc gcgaaatcgt ctgatgtttg ctcgcgaggg
     4201 gctagcggcc gtcgcattaa gtcatatgta aaaggagccc gatgtttcaa ttcagaaacg
     4261 tttttagtcg attaagtatc tggtggtgaa atgcttaata ggcaatattt tgataagtgt
     4321 gcggttcgcc acatcggcac tgcctgtaaa ttcccggttt gcgaataaaa gccggattga
     4381 acgttcatta tttgcgaggt ttgaagttca ggtttgaaac actttcgtct gttgccgcgg
     4441 ctaatcgatg cttcgaatca gcgtgatccg tgacgatccg aca
//
LOCUS       DATASH010000415         3901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3623271,
            whole genome shotgun sequence.
ACCESSION   DATASH010000415 DATASH010000000
VERSION     DATASH010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3623271"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VHV99_11560"
     CDS             complement(<1..405)
                     /locus_tag="VHV99_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013342604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX3636107.1"
                     /translation="MRKVLLLVLIFMTPACFAAHGIGTPLTADEIAAWNIDVAPDGRG
                     LPAGSGDVTTGAHVFAEKCAACHGSLGQGLIGDALVGGRGTLASAKPKRTVGSYWPYA
                     TTLFDYIRRAMPYNAPESLSADEVYALSAFLLN"
     gene            complement(398..1645)
                     /gene="soxC"
                     /locus_tag="VHV99_11565"
     CDS             complement(398..1645)
                     /gene="soxC"
                     /locus_tag="VHV99_11565"
                     /EC_number="1.8.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite dehydrogenase"
                     /protein_id="HEX3636108.1"
                     /translation="MTSRKSSPSPGRRRLLGSLAAGGFAASQAGAAELLNPLEIAPWS
                     KTPGAPILEHPYGLPSRYESDVVRRAAKAWPLPGAASSMTPLADLHGSITPNGLVYER
                     HHAGVPDIDPALHRLAIHGFVRNPKLFTMDDILRLPSESRIHFIECSGNTGNEWSGPS
                     GMPVQLTHGLLSCCEWTGVRLSTLLEEVGGTTGGSGWVLAEGADAAAMTRSLPLQRIA
                     DRALVAYAQNGERLRPENGYPLRLIVPGFEGNTHVKWLRRLKVVNAPLQTREETSKYT
                     NLLADGTARQFVFEMDAKSVITRPSPGHRLTTHGFYPISGLAWSGRGTIRQVDVSTDG
                     GATWRTARLETPVRDRALTRFEADWNWTGEPASILSRATDSTGYVQPTREALVAARGL
                     NSQYHYNGIQQWRIDAAGEVRNA"
     gene            complement(1653..2606)
                     /locus_tag="VHV99_11570"
     CDS             complement(1653..2606)
                     /locus_tag="VHV99_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042918 - alkanesulfonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX3636109.1"
                     /translation="MAQTPSGDLVKLRIGFQKSSTLIAILKARGTLERAVAPLNVRIE
                     WGEFASGLPLTEALNAGAVDFSADVADTVPVFAQAANANFVYVAQETPSPSAQAVIVH
                     RDAPIHTLADLKGKRIAVTKAAGSHYLLLAALARAKLLPADVKISYLAPADARAAFER
                     NDVDAWFAWDPYVASVEQDPGARILIRGDGLASYQRYYLASTPFAQAHPQVIDIVFKQ
                     LVEAGVWTKAHPQEAAKILAPVWGLDQATVERANTRRTYAVRAVVAKNFSEQQAIADA
                     FYQAKLLPKPVDTASALVWDFAAHKAVTANVATASLTAAQP"
     gene            2874..>3901
                     /locus_tag="VHV99_11575"
     CDS             2874..>3901
                     /locus_tag="VHV99_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX3636110.1"
                     /translation="MGSRPQFIDDLSRGPAGAALASPLGDEALLDTYSQTVISALERV
                     QQAVAFISVERRLPGDKNGRGAQGGSGSGFIFTPDGYLLTNSHVVHGATHIRVTLADG
                     ATFDADLVGDDPDSDLAVLRIGSAEALPHVELGESGKLRVGQIAIAVGNPLGLVQTVT
                     TGVVSALGRSLRSNSGRMIYDVIQTDAALNPGNSGGPLINSAGQVIGVNTAIIAGAQS
                     LSFATAIDTAKWVIMQLFAYGRVRRAYIGVAGTTMPISRRVQRYFELPSASGVHVMEV
                     VKGSPAALGGLRTDDRIVTIDTLPVDSVDSLQRSLDASRIDRAVKVTVLRGTQKLELD
                     VTPVEQAG"
BASE COUNT          732 a         1263 c         1221 g          685 t
ORIGIN      
        1 attcaacagg aacgcgctga gcgcatacac ctcgtcagcc gacagggact ccggcgcgtt
       61 gtacggcatc gcgcggcgaa tgtagtcgaa cagcgtggtg gcgtatggcc agtaactgcc
      121 aaccgtgcgc ttgggttttg cgctcgccag cgtgccccgg ccgcccacca gcgcatcgcc
      181 gatcaaaccc tgacccaacg aaccgtgaca ggctgcacac ttctccgcaa acacatgcgc
      241 acccgttgtt acgtcaccgc tacccgccgg aagaccgcgg ccgtctggcg cgacatcaat
      301 attccatgcc gcgatctcgt ccgccgtcag cggcgtgcca atgccgtgcg ccgcgaagca
      361 cgcgggcgtc atgaaaatca gcacgagtag cagcacctta cgcattgcgc acctcgcctg
      421 ctgcatcaat acgccattgc tggattccgt tgtagtgata ctgcgagttg agaccgcgcg
      481 cggcgaccag cgcttcgcgc gtgggttgaa cgtagccagt tgaatcggtg gcgcgcgaca
      541 ggatgcttgc cggttcaccg gtccagttcc agtccgcctc aaaacgcgtc agcgcacggt
      601 cacggaccgg cgtttccagc cgcgccgttc gccaggtcgc accgccgtcc gtcgagacgt
      661 ccacctggcg gatcgtccct cgtcccgacc acgccagtcc ggagatcgga taaaacccgt
      721 gcgtggtcag ccgatgtccc ggcgacggcc gcgtgatcac cgacttcgcg tccatctcga
      781 acacgaactg gcgcgcggtg ccatcggcga ggagattcgt gtacttcgat gtttcctcgc
      841 gcgtctgcaa aggggcgttg accaccttca ggcggcgcag ccacttgacg tgcgtattgc
      901 cctcgaagcc gggcacgatc agccgcaacg gatacccgtt ctcgggacgc aggcgttcac
      961 cgttttgcgc gtaagccacg agcgcgcgat ccgcaatgcg ctgcaacggc aggctgcgag
     1021 tcatcgcggc ggcatcggcg ccttcggcca gcacccaacc cgacccgccg gtcgtaccgc
     1081 caacttcctc cagcagcgtg gacaaacgca ctcccgtcca ttcgcaacac gacagaaggc
     1141 catgcgtgag ctgcacgggc atgccgctcg gaccgctcca ttcgttaccg gtattgcccg
     1201 aacattcgat gaaatgaatc cgcgactctg acggcaaccg caggatatcg tccatcgtga
     1261 aaagcttcgg attccgtacg aatccgtgaa tggccaaacg atgcagcgct ggatcgatat
     1321 ccggaacgcc cgcgtgatga cgctcgtaga cgagaccgtt cggtgtgatc gaaccatgca
     1381 ggtcggcaag cggcgtcatg gaggacgccg cgccgggcaa aggccaggct ttggcagcgc
     1441 ggcgcaccac atccgactcg tatcgtgacg gcagaccgta gggatgttct aggattggcg
     1501 cgccgggcgt cttcgaccat ggcgcaatct caagcggatt cagcaactcg gcagcgccgg
     1561 cttgcgacgc ggcaaacccg ccagcagcca gactgccgag cagacgtctg cggcccggtg
     1621 aaggtgagga ctttcgggag gtcatcgatg aactaaggct gcgcggcagt cagcgatgcc
     1681 gtcgccacat tcgcggtgac cgccttgtgc gcggcgaaat cccaaacgag cgcgctggcc
     1741 gtatcgacgg gcttgggcag cagcttcgcc tgatagaagg cgtcggcaat cgcttgttgc
     1801 tcgctgaagt tcttcgccac gaccgcacgc actgcatacg tccgccgcgt attcgcgcgc
     1861 tcgaccgttg cctgatcgag gccccacacc ggcgcgagga tcttcgccgc ttcctgcgga
     1921 tgcgccttgg tccagacgcc cgcttcaacc agttgcttga agacgatatc gatgacctgc
     1981 gggtgcgctt gtgcaaacgg cgtggacgca aggtaatacc gctgatacga cgcaaggcca
     2041 tccccgcgga tcagaatgcg cgcacccgga tcttgttcga cggaagcgac gtacgggtcc
     2101 cacgcgaacc acgcgtcgac gtcgttgcgc tcgaacgctg cgcgcgcgtc ggcgggtgcg
     2161 aggtagctga tcttgacatc ggccggcaag agcttggcgc gagccagcgc ggcgagcagc
     2221 aggtagtgac tgccggctgc cttggttacc gcaatgcgct tgcccttcag gtcggcgagc
     2281 gtgtggatgg gtgcgtcgcg atgcacgatc acggcttgag ccgagggcga cggcgtttcc
     2341 tgcgcgacgt agacgaagtt ggcgttagcc gcttgcgcga agacggggac ggtatccgcc
     2401 acgtcagcgc tgaagtccac cgcgcccgcg ttcagcgctt cggtgagcgg cagtccgctc
     2461 gcgaattcac cccattcaat acgtacgttg agcggcgcca cggcgcgttc cagcgtgccg
     2521 cgcgccttca ggatcgcgat cagcgtggac gatttttgaa agccgatacg cagtttgacg
     2581 aggtcgcccg aaggcgtttg agccatggcg ctcgccgcgc tcagggcagt cacggccagc
     2641 gcaaggcagg cgaccagacg ggtgaagatt ttcatggggc atcgcaggca gattaggcag
     2701 ccgaccatgc taacaacgcg cgcgtccagc actaaacgat caatcgtgat atcgattgca
     2761 gcggaaatgc tgagccggca atgcacccgg ctgtgtccct gggttgcatc cgccgggctt
     2821 gaaatacccg caagaatccc gcatctcatg atccatttat ccggagcacg atcatgggaa
     2881 gccgtcccca attcatcgat gatctctcac gcggccccgc gggcgccgcc ctcgcaagtc
     2941 cgctcggcga cgaagccttg ctggacactt actcccagac cgtgatcagc gcgctcgaac
     3001 gcgtgcagca agcggtcgcc ttcatttcag tcgaacggcg tttgcccggc gacaaaaacg
     3061 gccgcggtgc gcagggaggc tcgggctcgg gattcatctt cacgcccgac ggttatctgc
     3121 tgacaaatag ccacgtcgtg cacggtgcaa cgcatatccg cgtgacgctc gcggacggcg
     3181 ccacgttcga cgccgatctc gtcggcgacg atccggacag cgacctcgcg gtgctgcgca
     3241 ttggctcagc cgaggcgtta ccgcacgtcg aactgggtga gtccggcaag ctgcgagtcg
     3301 ggcagatcgc gatcgccgtg ggcaaccctc tcgggctcgt tcagacggtc acgaccggcg
     3361 tggtctcggc gctcggacgg tccttgcgat cgaactccgg acgcatgatc tacgacgtga
     3421 tccagaccga cgcagcgctc aatcccggca actcgggcgg cccgctgatc aactcggcgg
     3481 gtcaggtgat cggcgtgaat acggcgatca ttgccggcgc tcaatcgctc tcgttcgcga
     3541 ctgcaatcga taccgccaag tgggtcatca tgcagctctt cgcatacggg cgcgtgcggc
     3601 gtgcctacat tggcgtggcc ggcacaacca tgcccatctc gcgccgcgtg cagcgttatt
     3661 tcgaactgcc gtcggcgagc ggcgtgcatg tgatggaagt cgtcaaagga agtcccgccg
     3721 cgctcggcgg attgcgtacc gatgaccgga tcgtaactat cgacacactg ccggtagata
     3781 gcgtagatag cttgcagcgc tcgctggatg catcgcgtat cgaccgggcg gtgaaggtaa
     3841 ccgtcttgcg cggcacgcaa aagctggaac tggacgtgac gccggtcgaa caggcgggat
     3901 a
//
LOCUS       DATASH010000416         2218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2041796,
            whole genome shotgun sequence.
ACCESSION   DATASH010000416 DATASH010000000
VERSION     DATASH010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2041796"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /gene="tyrS"
                     /locus_tag="VHV99_11580"
     CDS             complement(<1..654)
                     /gene="tyrS"
                     /locus_tag="VHV99_11580"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX3636111.1"
                     /translation="MDLLEKGAAEIIRVPELRERLEKSRQTGVPLRVKAGFDPTAPDL
                     HLGHTVLMRKLKHFQDLGHQVIFLVGDYTSLIGDPTGRNVTRKPLTREQIDENAKTYT
                     AQVFRILDREKTEVRFNSEWLGKLSFEDIIRLAAKFTVSQMLERAEFHKRFQEEQPIS
                     LHEMMYPLAQGYDSVALKADVELGGTDQKFNLIAGRELQRDYGQPSQIVLMTPIIEGL
                     "
     gene            complement(730..2124)
                     /locus_tag="VHV99_11585"
     CDS             complement(730..2124)
                     /locus_tag="VHV99_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636112.1"
                     /translation="MLVEGSVYHGVWLECAPQEGDVYDAAGLEAARRLARNNHLIFFA
                     LQKEDGQLPCSVKDGGPAFGQIQMVVPIAATAWELAQRTGDHELVEKAYAACTRWDEW
                     LRRYRDTRATGLCEGFCTYDTGQDNSPRWKGLANRCPDSDARKCPEGAGMPRLCPDLS
                     ATVYGGRVALAAMAKALGKPGEADRWTEDAETIRQLIIGKLYDPVDGAFYDLDTDNKF
                     VRVRSSTIMTVLGEHVAEPGVFEDVWNRQAHNPKAFWTPYPFPSVAIDDPQFVRPIPR
                     NSWGGAAQALTALRAPRWMEHYGKSAELGELMHRWIEALYRANGEFRQQMDPVTGEFT
                     VADPGGYSPAALVCLDFVWRLHGVRQQGELLEWNVRPPSERGRSHFSRKIGTATAELN
                     YEGRHAELRIGGERIARVSGVVRLLTKKNGDVDSAFGIAGNEEQVTVDVAGSLKRAFR
                     IAPNDRVVLRASTS"
BASE COUNT          389 a          815 c          608 g          406 t
ORIGIN      
        1 cagtccctcg ataatcggcg tcatcagcac gatctgcgat ggctggccgt agtcgcgctg
       61 caactcgcgc ccggcaatca ggttgaactt ctggtccgtc ccgcccagct ccacatccgc
      121 cttcagcgcc accgagtcgt atccctgcgc cagcggatac atcatttcgt gcagcgagat
      181 cggctgctcc tcctgaaacc gcttgtgaaa ctcggcccgc tccagcatct gtgaaacggt
      241 gaacttcgcc gcgagccgga tgatgtcctc aaagctcagc tttcccagcc actccgagtt
      301 gaagcgcacc tcggtcttct cgcggtcgag aatgcgaaac acctgcgcgg tataggtctt
      361 cgcgttctca tcaatttgct cgcgcgtgag cggcttgcgc gtgacgttgc gccccgtcgg
      421 atcgccgatc agcgacgtgt agtcgccgac cagaaagatc acctggtgcc cgaggtcctg
      481 aaagtgcttc agcttgcgca tcagcaccgt atggcccagg tgcagatcgg gcgcggtggg
      541 atcgaacccc gccttgacgc gcagcggcac gcccgtttgc cgcgacttct cgaggcgctc
      601 tcgcagctcc ggcacgcgaa taatctccgc cgcgcccttt tcgagcagat ccatctgctc
      661 atcgacagga agaaaggtgg acatagtctg gtttcaggta tcccggtcag tataaatttc
      721 gcggcgctct cacgaagtgg aagcgcgcaa aaccacgcga tcgttcggcg caatgcggaa
      781 cgcgcgcttc agcgatccgg caacgtcgac cgtcacctgt tcctcgttgc cggcgatgcc
      841 gaacgccgaa tcgacgtcgc cattcttctt cgtcagcagc cgcaccacgc cggagacgcg
      901 cgcgatgcgt tcgcccccga tccgcagctc cgcatgcctg ccttcgtaat tcagctccgc
      961 cgtcgccgtg ccaatcttcc gagaaaaatg cgaacgcccg cgctccgatg gcggccgcac
     1021 gttccactcc agcagttctc cctgctgtcg cacgccgtgc agccgccaca caaaatcgag
     1081 gcacaccagc gccgccggtg aatacccacc aggatcggcc acggtgaact cgcccgtcac
     1141 cggatccatc tgctgccgga actccccgtt cgcgcgatag agcgcctcga tccagcgatg
     1201 cattagctca cccaactccg cgctcttgcc gtagtgctcc atccagcgcg gcgcgcgcag
     1261 cgccgtcagc gcctgcgctg ccccgcccca cgagttgcgc ggaatgggcc gcacaaactg
     1321 cggatcgtcg atcgccaccg atggaaacgg atacggcgtc cagaacgcct tcgggttgtg
     1381 agcctgccgg ttccacacat cttcaaagac gccaggctcc gccacatgct cgcccagcac
     1441 cgtcataatc gtcgagctgc gcactcgcac aaacttgttg tcggtatcga gatcgtagaa
     1501 cgcgccgtcg accggatcgt agagctttcc aataatcagc tgccggatcg tctccgcatc
     1561 ctcagtccac cggtccgcct cgcccggttt gcccagcgcc ttcgccatcg ccgccagcgc
     1621 cacgcgtccg ccataaaccg tcgccgaaag gtccggacac aagcgcggca tccccgctcc
     1681 ctccggacac ttccgcgcat cgctgtccgg gcagcggttc gcgaggccct tccagcgcgg
     1741 gctgttgtcc tgccccgtgt cgtacgtgca gaagccctcg cacaacccgg tcgcgcgcgt
     1801 gtcgcgatac cgccgcagcc actcatccca gcgcgtgcat gccgcgtagg ccttctccac
     1861 cagctcgtgg tcgcccgtcc gttgcgccag ctcccacgcg gtggccgcaa tcggcaccac
     1921 catctggatc tgcccaaacg ccggcccgcc atctttcacg ctgcagggca gctgcccatc
     1981 ttccttttgc agcgcgaaga agatcagatg attgttgcgc gcaagccgcc gtgccgcctc
     2041 cagcccggcg gcgtcataaa cgtcgccttc ctgcggcgcg cactccagcc agactccgtg
     2101 atacacgctg ccctcaacca gcaccggtcc cggatacgac gggaacgtca cggcatttcc
     2161 cgtgagcgtc gcgagcgcgg cgtcgtagat atgctgcaat ccggaatctt ccgtccgg
//
LOCUS       DATASH010000417         4192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5205664,
            whole genome shotgun sequence.
ACCESSION   DATASH010000417 DATASH010000000
VERSION     DATASH010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5205664"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..744
                     /locus_tag="VHV99_11590"
     CDS             187..744
                     /locus_tag="VHV99_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HEX3636113.1"
                     /translation="MKAIFPALAIATTGLLGLASFGAHAADGTITITGTVSDTTCSIN
                     GTQTGASADKSITLPEVTAGTLSATGKTAGTSSPTDLALTLSGCSGAATKAIAHFENG
                     STVDQNSGNLLNAGTATNVQVQLLNSKMQPINVTTNSNNQLATDGVTITGGAASPKYF
                     AQYYATGKATAGTVSSQVQYTMQYQ"
     gene            823..1566
                     /locus_tag="VHV99_11595"
     CDS             823..1566
                     /locus_tag="VHV99_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HEX3636114.1"
                     /translation="MKLLGKMTMGIGFACALLAGSAHASVTIGGTRVVYPLEQREVTV
                     KLDNDSRNPSLVQVWMDDGNADAKPGEIKVPFVITPPIFRMDPKKAQTLRMMYSGEAL
                     PQDRESVYWLNVLDIPPKASGATDANVLQLAYRTRIKVFVRPAKLPGKPEDAPAQLSW
                     KVTAAPSGKGQAVSVSNPTPYYVSFSEVDVESSGHTYKNEEGGMVAPHATTVLPVPKM
                     DSVPAGAKVHYMAISDYGGALNGDATLGQ"
     gene            1700..>4192
                     /locus_tag="VHV99_11600"
     CDS             1700..>4192
                     /locus_tag="VHV99_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HEX3636115.1"
                     /translation="MKLSPRHQSHARASEFSGAFGLSPVAAVVMSAFAVLGGVQSASV
                     NAAEASDAGASVQFDTTFLRTDQSQSVDVSRFTSGNLVQPGVYSVDLFVNDVRVARQD
                     VRFVATRDGKSARACFSREMLEKLGVDFAKVGADTGAKVPGHTSAEAAGAKDTHSAAD
                     ASANADECMDLGATVPEATVDFDFSEQKLTLSVPQKYMRNAARGYVPPDMWQNGVNAG
                     FVSYNANTYHTAGSGTQSTQSYLGLNAGVNIGAWHFRHQSSLSQGTGQGTHFDNIATY
                     VQHDVAALKSQVTVGDGSTTGDVFDSVQFRGAQIATDDRMLPESLRGYAPVVRGTAES
                     NARVTVRQNGQVIYETSVSPGPFEINDLYATGYGGNLDVMVTEADGRTKTFTVPFASV
                     AQSLRPGTTRFSVTAGQLRNSTLESQPNFAQFTLQRGLTNLVTAYGGAVAANGYVAAD
                     LGAALNTKFGAFSADVTGAQTQVPNQSTMRGTSLRIGYSKFIDPTNTNIAVAAYRYST
                     AGFLNLSDASSVRDLAMRGGDTNSVYRQRNRFQLTVNQNIKDHGSVFLSASSQQYWNH
                     AGSDTFYQAGYTNSFKYGTYSVTAGRTRNADGSMSNQYMVSTTLPLGHSQHTPLLSTN
                     LSSSGGNTNLQASVSGSLGDASQYSYNAYGTYGNGSGSSTGNAGVSGAWRAPYAQMTA
                     SASAGSGSRQASAGISGSVVAHPGGVTFSQTVGDTFGIVEAPGAAGASVTSAPGVKLN
                     GRGYAVVPYLTPYGMNVVDIDPNGTSTDVEFESTSEQAVPRLGSVVMIKYKTVSGRAA
                     LIRAQQLGDKALPFGADVVDGNGRNVGVVAQDS"
BASE COUNT          891 a         1225 c         1305 g          771 t
ORIGIN      
        1 caaataagaa tcgtcctagg tacataggaa tcttgaaacc ctaacattgg tttgtgcctg
       61 aacgagaggc caatagttcg aagccgatac cgagaacatt ggagcgccca ggaacaaatg
      121 gcgggtctca cccaatatcg agtcgccaag gattcacaaa tttcttaact cactggagca
      181 gcaaacatga aagcaatttt ccccgcactc gcaatcgcca ccaccggtct gttgggtctt
      241 gcatcgttcg gcgcccacgc agcggacggc acgatcacca tcaccggcac ggtgtcggat
      301 accacgtgct cgatcaacgg cactcaaacc ggcgcctccg ctgacaagtc gatcacgctg
      361 cctgaagtga ccgcaggcac gctgtcggcg accggcaaga cggcaggtac gtcgagcccg
      421 accgatctgg cgctgaccct gagcggctgc tcgggtgcgg caaccaaggc gatcgcgcac
      481 ttcgaaaacg gttcgacggt agaccagaac agcggcaacc tgctcaacgc cggtacggcg
      541 acaaacgtgc aagtccaatt gctgaactcg aagatgcagc cgatcaacgt cacgaccaac
      601 tcgaacaacc agttggcaac ggacggcgtg acgatcaccg gcggtgcggc aagcccgaag
      661 tacttcgcac agtactacgc gacgggtaag gcaacggccg gtacggtcag ctcgcaagtg
      721 caatacacga tgcaatatca gtaagcgcgg gccgaggcgt tcgctaaggc gaacgccttt
      781 tttacggcct ttttttaaga ttattttttg acaggtgatg tgatgaagtt gctcggaaaa
      841 atgacgatgg gaatcggctt tgcatgtgcg ttgctcgcgg gcagcgcaca tgcaagcgtg
      901 acgatcggcg gtacgcgagt ggtgtatccg ctcgagcagc gcgaggtcac cgtcaagctg
      961 gataacgaca gccgcaaccc gtcgctcgta caagtatgga tggatgacgg taacgcagac
     1021 gcgaaacccg gcgagatcaa ggtgccgttc gtcattactc cgccgatctt ccgcatggac
     1081 cccaagaagg cgcagacgct gcgcatgatg tatagcggcg aagcattgcc gcaagaccgt
     1141 gagtccgtgt attggctcaa cgtgctcgat attccgccga aggcaagcgg tgcaacggat
     1201 gccaacgtgc tgcagttggc ctatcgcacg cgcatcaagg tatttgtgcg cccggcaaag
     1261 ctgccgggca agccggaaga cgcgccggct caattgagct ggaaggtgac ggccgcgccg
     1321 agcggcaagg gccaggccgt cagcgtatcg aatcctacgc cgtactacgt gtcgtttagc
     1381 gaagtcgatg tggaaagcag tggacacacc tacaaaaacg aagaaggcgg catggttgcc
     1441 cctcatgcga cgacggtgct gccggtgcca aagatggact ccgttccggc cggcgcgaag
     1501 gttcactaca tggcgatcag cgactacggt ggcgccctca acggcgacgc gacactcggc
     1561 caatagaccg cggcagtttg ccggtcgggc ggcagcatcc cgcccttagt caaacccatt
     1621 tttgagcacg gcgtgcgttg cacaggcaac gcacaaggac gacgcacgcc tgtttcaaac
     1681 attagaagtt actcagatca tgaaactttc acctcgtcat caatcgcatg ctcgtgccag
     1741 cgaattttct ggcgcgttcg gcctgagccc tgtggccgca gtggtcatgt cggctttcgc
     1801 ggtgctgggc ggcgtccagt cggcgagcgt gaatgcagcg gaagccagcg acgcgggcgc
     1861 ttcggttcag ttcgatacga cgtttctgcg taccgaccag agccaatccg tggacgtctc
     1921 gcgtttcacg agcggcaacc tggtacagcc gggcgtgtat tccgtcgacc ttttcgtcaa
     1981 cgacgttcgc gttgcacgcc aggacgtgcg ctttgtggcg actcgcgatg gaaagagcgc
     2041 gcgtgcttgc ttcagccgcg agatgctgga aaaactcggt gtcgacttcg ccaaggttgg
     2101 tgccgatacg ggcgcaaagg taccggggca cacgtctgcc gaagcggccg gcgccaagga
     2161 cacgcattca gcggccgatg caagcgccaa tgctgacgaa tgcatggatt tgggcgcaac
     2221 ggtgcccgag gcgaccgtcg acttcgactt ctctgaacag aagcttacgc tgagcgtgcc
     2281 gcagaaatac atgcgcaacg ccgcacgcgg ctatgtgcct ccggacatgt ggcaaaacgg
     2341 cgtgaacgcg ggcttcgtca gctacaacgc gaatacctat cacaccgccg gctcgggcac
     2401 gcagtcgaca caaagctatt tgggcctgaa tgccggcgtg aacatcggcg cgtggcactt
     2461 ccgtcaccaa tcttcgttgt cgcaggggac gggccaaggc acgcatttcg acaacatcgc
     2521 gacctatgtg cagcacgacg ttgccgcgct gaaatcgcaa gtgacggtgg gtgacggttc
     2581 cacgacgggc gacgtgttcg acagcgtgca gttccgtggc gcgcagattg ccaccgatga
     2641 ccgcatgttg ccggaatcgc tgcgtggcta tgcgccagtg gtgcgtggca cggcggagtc
     2701 gaatgcacga gtgacggtcc gtcagaatgg ccaggtgatc tatgaaacca gcgtgtcgcc
     2761 gggtccgttc gaaatcaacg acctgtacgc aactggctac ggcggaaatc tcgatgtgat
     2821 ggtgaccgaa gcggacggcc gcacgaagac ctttaccgtg ccgttcgctt cggttgcaca
     2881 gtcattgcgg ccgggcacca cgcggttctc ggtgaccgcc ggacaattgc gcaacagcac
     2941 gctcgagtcg cagccgaatt ttgcccagtt cacgttgcag cgtggcctca cgaacctggt
     3001 gaccgcgtac ggcggcgccg tcgccgcaaa cggttatgtc gcggctgacc tcggcgccgc
     3061 gctgaacacg aagttcggcg ctttctccgc cgatgtcact ggcgcacaaa cccaggtgcc
     3121 gaaccagagc acgatgcgtg gtaccagctt gcgcattggc tacagcaaat tcatcgatcc
     3181 gaccaacacg aacattgccg tggctgcgta ccgttattcg accgcgggct tcctgaatct
     3241 gtcggacgcg tcatcggtgc gtgatctggc catgcgcggc ggcgacacga attcggtcta
     3301 tcgccaacgc aatcgtttcc agcttacggt caaccagaat atcaaggatc acgggtcggt
     3361 gttcctgagt gcgtcgtcgc agcagtactg gaatcacgcc ggtagcgata cgttttatca
     3421 ggcagggtat acgaacagct ttaagtacgg cacctatagc gttaccgcgg gacggacccg
     3481 caacgcagac gggtcaatgt ccaaccagta catggtcagc acgacgctgc cgcttggcca
     3541 cagccagcat acgccgttgc tgtcgaccaa cctgtcgagc tcgggcggta acaccaatct
     3601 gcaagccagc gtgagcggct cgcttggcga tgccagccag tattcgtaca acgcgtacgg
     3661 gacgtatggc aacggcagtg gcagcagtac cggcaacgct ggtgtgagtg gtgcatggcg
     3721 cgcgccgtat gcgcagatga cggcatcggc aagcgcgggc tcgggttcga ggcaggcatc
     3781 ggccggtatc agcgggtcgg ttgtcgcgca tccgggcggc gtgacgtttt cgcagacggt
     3841 tggcgatacg ttcggcatcg ttgaagcacc gggcgctgcg ggcgcaagcg tgaccagcgc
     3901 gccgggcgtc aagctcaatg gccgcggcta tgcagtggtg ccttacctga cgccttatgg
     3961 catgaacgtc gttgacatcg acccgaatgg cacctcgacc gacgtcgaat tcgaatcgac
     4021 ctcggagcag gctgtgccgc gtctcggctc ggtggtgatg atcaagtaca agacggtgag
     4081 cggacgtgcg gcgctgattc gcgcgcagca actcggcgac aaggcgctgc cgtttggtgc
     4141 ggatgtggtt gatgggaatg gtcgcaacgt gggcgtcgtg gcgcaggaca gc
//
LOCUS       DATASH010000418         4464 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2241258,
            whole genome shotgun sequence.
ACCESSION   DATASH010000418 DATASH010000000
VERSION     DATASH010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2241258"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..1478)
                     /gene="pmbA"
                     /locus_tag="VHV99_11605"
     CDS             complement(108..1478)
                     /gene="pmbA"
                     /locus_tag="VHV99_11605"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HEX3636116.1"
                     /translation="MAADMDVKQRFFPHTQDELKEIASDILRHAKSLGGTDAATEISE
                     GDGLSVSVRRGEVETIEHNRDKMVGVTVFIGNKRGNASTSDFSSQALKDTVAAAYNIA
                     RFTAEDDCAGLAEAELLETAPRDLDLYHPWNLSADEAVEIARRSEDAAFATDPQIKNS
                     EGASVSAQHSQFVLATSRGFLAGYPYSRHYIACAPIAGSGRNMQRDDWYTSTRSAGDL
                     ADPEAVGRYAAQRALSRIGARGLDTRKVPVLFEAPLAAGILGAFVQATSGGALYRKTS
                     FLVDSLGKPVFAPHVQVVEDPHVARAMGSAPFDEEGVRTKQRSVVKDGVVEGYFLSTY
                     SARKLGMQTTGNAGGSHNLSLRSSNTRPEDDFEEMLRKLGTGLLLTELMGQGVNYVTG
                     DYSRGASGFWVENGKIQYPVEEITVASTLQEMFRHIVAIGADTITRGTKETGSVLIER
                     MTIAGQ"
     gene            1897..2508
                     /gene="yjgA"
                     /locus_tag="VHV99_11610"
     CDS             1897..2508
                     /gene="yjgA"
                     /locus_tag="VHV99_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HEX3636117.1"
                     /translation="MTRKTRIQPIESAEPEVDENGYDRPSKSQLKREMHELQELGSAL
                     IALPKDALKRMPMPEKLDDAVREARRITDHEGKRRQVQYVGRVMRSLLDGETAALRTA
                     LDTYNGVNKAETAKLHWIERTREKLLADDAALTDFIRQHPNADPQQGRTLIRNARKEA
                     QQSKPPRYFRELFQWIKNADGPSAGADSDAEEGLEDDDDDRDA"
     gene            2489..3145
                     /gene="mog"
                     /locus_tag="VHV99_11615"
     CDS             2489..3145
                     /gene="mog"
                     /locus_tag="VHV99_11615"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HEX3636118.1"
                     /translation="MTTATHRPVPKVARRHPDEIVIGLVSISDRASTGVYEDKGIPAL
                     QEWLGAALTSPFQVVTRLIQDDAPTISTTLIELVDEVGCDLVLTTGGTGPSRRDVTPE
                     ATLAVATKEMPGFGEQMRQISLNFVPTAILSRQVAVIRETADRAALIINLPGQPKSIK
                     ETLEGLRDAEAGGAVKVPGIFAAVPYCIDLIGGPYVETNADVVKAFRPKNAVRAPRQD
                     "
     gene            complement(3205..3813)
                     /gene="orn"
                     /locus_tag="VHV99_11620"
     CDS             complement(3205..3813)
                     /gene="orn"
                     /locus_tag="VHV99_11620"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEX3636119.1"
                     /translation="MTDILASSEQPLLVRSDMNLIWLDMEMTGLEPDSDRIIEIAVVV
                     TNSTLDRMVEGPVLAIHQSDETLAKMDQWNQNTHGRSGLIDRVKASTVSEADATEQIR
                     DFLGAYVPPGKSPMCGNSICQDRRFMARWMPDLERFFHYRNLDVSTLKELCRRWQPGI
                     YKGFQKRALHTALADIHESIDELKYYREHFLIPAASESPAAE"
     gene            3995..>4464
                     /locus_tag="VHV99_11625"
     CDS             3995..>4464
                     /locus_tag="VHV99_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family peptidase"
                     /protein_id="HEX3636120.1"
                     /translation="MPTLYFTVLFAVAVVAMVGTKLWLASRQIRFVAGHREQVPSQFA
                     GTIALTAHQRAADYTVERTRLTMIEIVVSAAVLIGLTLLGGVQALDLGISDWLGRGYA
                     GQIALVAAVIAITSVIDLPFDYYRQFVVEQRFGFNRMSKGIFFVDRIKGVLLGAA"
BASE COUNT          786 a         1476 c         1400 g          802 t
ORIGIN      
        1 caactgcttg cgtggcgttt ctgtttgctg cttcttgccg cgggcagccc aatccggcgg
       61 ctaccggtcc acgagcagag cagtccgcag ccggctggcc aaaccgctca ctgcccggca
      121 atcgtcatcc gttcgatcaa caccgagccc gtttccttcg tgccgcgcgt gatcgtatcc
      181 gcaccgatcg cgacgatatg gcggaacatc tcctgcagcg tgctcgccac ggtgatttcc
      241 tcgaccggat actggatctt gccgttctcg acccagaaac ccgacgcgcc gcgtgaataa
      301 tcgcccgtca cgtagttcac cccctgcccc atcagttcgg tcagcaacag ccccgtgccg
      361 agcttcctca gcatttcctc gaagtcgtct tccgggcgcg tgttcgagct gcgcagcgac
      421 agattgtgcg agccgccggc attgccggtg gtctgcatgc cgagcttgcg cgccgaatag
      481 gtcgagagga aataaccctc caccacgccg tccttcacca ccgagcgctg cttcgtacgc
      541 acgccttctt cgtcgaacgg cgcactgccc atggcacgcg cgacgtgcgg atcttcaacc
      601 acctgcacgt gcggcgcgaa caccggcttg ccgaggctat cgacgaggaa cgaggtcttg
      661 cggtacagcg cgccgccact cgttgcctga acgaacgcgc cgagaatgcc ggcggcgagc
      721 ggtgcttcga acagcaccgg caccttgcgc gtgtcgagac cgcgcgcgcc gatgcgcgac
      781 agcgcccgtt gcgccgcgta gcggcccacg gcttccggat cggcgaggtc gcccgcgctg
      841 cgggtcgacg tgtaccagtc gtcgcgttgc atattgcggc cgctgccggc gatcggcgcg
      901 cacgcgatgt agtggcgcga gtacggatag ccggcgagga agccgcgcga cgtagccagc
      961 acgaattgcg agtgctgcgc cgagacgctc gcgccttccg agttcttgat ctgcggatcg
     1021 gttgcgaacg cggcgtcttc cgagcggcgg gcgatttcca ccgcttcgtc cgcggacaga
     1081 ttccacgggt gatacagatc gagatcgcgc ggcgcggttt cgagcaattc tgcttcggcg
     1141 aggcccgcgc agtcgtcttc agccgtgaag cgcgcgatgt tgtacgccgc cgccaccgtg
     1201 tccttcaatg cctgcgacga aaaatccgag gtgctcgcgt tgccgcgctt gttgccgatg
     1261 aacaccgtca cgccgaccat cttgtcgcgg ttgtgctcga tcgtctcgac ttcgccgcgc
     1321 cgcacggaga cggacaggcc gtcgccttcg gaaatctcgg tcgccgcgtc ggtaccgccg
     1381 agcgacttcg cgtgacgaag gatgtccgag gcgatttcct tcagttcatc ctgggtgtgc
     1441 ggaaaaaagc gctgcttgac gtccatgtct gctgccattg tcgttgccgt cctgttcggg
     1501 gccgagcggc cctgtgtgtt cgcctatgcc gcgcacgtgc gtacgcatct gcaagtgcca
     1561 tttacccgtg catttgcgtg cgcgtttgcg gggttacgcc tcccgcgatc atagcaaggt
     1621 acgcgaagct gtacctggca ttcgaggggt tcgctcgatg agcctgctgg ccgaacacat
     1681 ttgcccgcct cggcgacacg tgcctgcgct gccagcccgc tcagtatcgc cgcagccgct
     1741 ggatgcgcgc attgaacgaa ggcatcgccc cacctcgacg ccgtgcccgc gcgacccgac
     1801 cggccgtcag cgccgcgccc gcaaaaacct cgcgcgcgag cccttgtgca cggcagcacg
     1861 gcaggttcgg cgtgcgcggc acgctacaat accggtatga cacgcaaaac ccgcattcaa
     1921 cccatcgaat ccgccgagcc ggaagtcgac gaaaacggct acgaccgtcc cagcaagtcc
     1981 cagctcaagc gcgaaatgca cgagctgcag gaactgggct cggcactgat cgccctgccc
     2041 aaagacgcgc tcaagcgcat gccgatgccc gaaaagctcg acgatgccgt gcgtgaagcc
     2101 cgccgcatca ccgatcacga aggcaagcgc cgccaggtgc agtacgttgg gcgcgtgatg
     2161 cgctcgctgc tggacgggga aaccgccgcc ctgcgcaccg cgctcgacac ctataacggc
     2221 gtcaacaagg cggaaacggc caagctgcac tggatcgagc gcacccgcga gaaactgctt
     2281 gccgacgacg ccgcgctgac tgacttcatc cgccagcacc cgaatgccga cccacagcag
     2341 ggccgcacac tgatccgcaa cgcccgcaag gaagcgcagc aaagcaagcc gccgcgctac
     2401 ttccgcgagc tgttccagtg gatcaagaac gcggatgggc cgtctgccgg cgcagattcc
     2461 gacgctgaag aaggcctgga agacgacgat gacgaccgcg acgcataggc ccgtgcctaa
     2521 ggtggcacgt cggcatcccg acgaaatcgt gatcggcctc gtgtcgatca gcgaccgcgc
     2581 gtccaccggc gtgtacgagg acaaaggcat tccggcgctg caggaatggc tcggcgctgc
     2641 cctgacttcg ccttttcagg tggtgacacg cctgattcag gacgacgcac ccaccatttc
     2701 caccacgctg atcgaactgg tggacgaagt gggctgcgat ctggtgttga caacgggtgg
     2761 caccggcccg tcacgccgcg atgtgacgcc ggaagcgact ttggcagtgg ccacgaagga
     2821 aatgccgggt ttcggcgagc agatgcgcca gatcagcctg aatttcgtgc cgacggcgat
     2881 tctgtcgcgc caggtggcag tgatccgcga aaccgcggac cgcgcggcgc tgatcatcaa
     2941 tctgccgggt caaccgaaat cgatcaagga aacgctggaa ggcttgcgcg acgccgaagc
     3001 aggcggcgcg gtcaaggtgc cgggcatttt cgccgcggtg ccctattgca tcgacctgat
     3061 cggcggcccg tatgtggaaa cgaacgccga cgtggtgaaa gcgttccggc cgaagaacgc
     3121 ggtgcgcgca cctcggcagg attgagcggg aacacgcgtc agcggcgtga ctccgctgtc
     3181 agcgccaccg ggtccgcggc tgcgttactc cgctgccggc gattcagaag ctgccggaat
     3241 caggaagtgc tcgcggtagt acttgagttc gtcgatcgat tcatggatat ccgccagcgc
     3301 cgtatgcaag gcgcgtttct ggaaaccctt gtagatcccc ggctgccagc ggcggcacag
     3361 ttccttcagc gtgctgacgt cgaggttgcg gtaatggaaa aagcgctcca gatccggcat
     3421 ccagcgcgcc atgaagcgac ggtcctggca gatcgagttg ccgcacatcg gcgatttgcc
     3481 gggcggcacg taagcgccga ggaaatcgcg gatctgctcg gtggcatcgg cttcgctgac
     3541 cgtggacgcc ttcacccggt cgatcaggcc cgaacggcca tgcgtgttct gattccactg
     3601 atccattttg gcgagcgtct cgtcgctctg atgaatcgct agcaccggac cttcgaccat
     3661 cctgtcgagc gtcgaattcg tcaccaccac cgcgatttcg atgatgcggt cggagtcggg
     3721 ctcgagcccg gtcatttcca tgtccagcca gatgagattc atgtcgctgc gtaccagcag
     3781 cggctgttcg gaggatgcga ggatgtcggt catgaaggca atcctgatgg ccaggccggc
     3841 gacttcgctg gaagcctgcc ggcgcgcggc gcctgttcgt ttgagatggg tttaagcggt
     3901 cgatttgtgc cggtttaagc cggtttgacc gtatcccccg ccgcgaggcg ggaagaaaca
     3961 tataattctc gcatagatac cacggaatcc ccggatgcct accttgtact tcaccgttct
     4021 gttcgccgtc gccgtcgtgg cgatggtcgg caccaaactg tggctcgcgt cgcggcagat
     4081 ccgcttcgtg gccggccatc gcgagcaggt gccgagccag ttcgccggca ccatcgcgct
     4141 caccgcgcac cagcgcgccg ccgactacac ggtcgagcgc acccgcctca cgatgatcga
     4201 gatcgttgtc agcgcggccg tgctgatcgg cctcacgctg ctcggcggcg tgcaggcgct
     4261 cgatctgggc atctccgact ggctcggccg cggctacgcc ggccagatcg cgctggtcgc
     4321 ggcggtgatc gcgattacca gcgtgatcga cctgccgttc gactactacc ggcagttcgt
     4381 ggtcgagcag cgcttcggct tcaaccgcat gagcaagggc attttcttcg tcgaccggat
     4441 caagggcgtg ctgctcggcg ccgc
//
LOCUS       DATASH010000419         8087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6195410,
            whole genome shotgun sequence.
ACCESSION   DATASH010000419 DATASH010000000
VERSION     DATASH010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 8087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8087
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6195410"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            337..2127
                     /locus_tag="VHV99_11630"
     CDS             337..2127
                     /locus_tag="VHV99_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3636121.1"
                     /translation="MRLTTKGLLLIAIPALFELALLSGLVKAQSDAAQAEQWAIHSED
                     VLRQTTAILDPVLGESVALRGAVLANDTRFTTPVTFWMDVDRRIDQLAELVADNPAQV
                     ERVVQVRQEVQGYRQWSDRVQDMLHSGRRRDLLERFRELAPADVLDSFRQQVAAFQAE
                     ERHLDTLRSNAADAARERQQMLIVAAVFGSLLFVALAVWLFTRGVRGRLAVLSDNAGR
                     LAGNEPLAPLSPGRDEIAQLDLTLHETSRRLLEAERIQARFQADLARRTGELARINET
                     LRQQTQENEMFIYSVSHDLRAPLVNLQGFSKELIRACDELRAMLRQSSLAAQPRQRIE
                     RVVDEDIGEALHFLQTAVLRASHIIDALLRLSRVGRVEYRQQKVEVRDFVPRVVDAMQ
                     VSIRARRARVIVEDLPAVWGDPTALEQVFANLIGNAVNYLDPEREGRIEIGTTPAPPG
                     VHSLRIFYVRDNGLGIPAVALPRLFNAFQRLHGNVAAGEGIGLALVRRVVERHGGRVW
                     AESKEGMGTTFYLSLPEAEARMAQLSVETHAASADGVHAVRGAREGRAGLADRDGAGL
                     SGGAATHVANTANTASAANTADAAPGISTR"
     gene            complement(2114..2440)
                     /locus_tag="VHV99_11635"
     CDS             complement(2114..2440)
                     /locus_tag="VHV99_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636122.1"
                     /translation="MELTDWIVILAVTLMAIAFFCARTPANWSRENRARRLAGMRMLV
                     QAACSLWAALLIAARGLFALDGVAGLHPAPLEALTGVAILIACGCYWSIRGSKLLKPR
                     RLFTAY"
     gene            complement(2576..3157)
                     /locus_tag="VHV99_11640"
     CDS             complement(2576..3157)
                     /locus_tag="VHV99_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006024552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEX3636123.1"
                     /translation="MAAKKILFLTGDFAEDYETMVPFQALQAVGHVVDAVCPNKKAGE
                     KIKTAIHDFEGDQTYTEKPGHQFTLNATFDEADPRQYDALAIAGGRAPEYLRLNHKVI
                     ELVRQFAEAGKPIAAICHAAQLLAAADVIRGKRISAYPACAPEVKLAGGEYADIPVDA
                     AITDANFVTAPAWPAHPEWLRQFLVLLGTRIEL"
     gene            3336..3812
                     /locus_tag="VHV99_11645"
     CDS             3336..3812
                     /locus_tag="VHV99_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636124.1"
                     /translation="MTLSIRSPLLRRAAIVAIVFGGLAACQKNDASAGQVGGKLDDAA
                     QAAGQKLDQAASYVGQQVDATKAAAQQNLESASAPSITIDPASLASTAQANLQGAASA
                     TNAQLGKAAALTGQSLESAGRKLQEWSAQSSASSAGVSASPSASSDSGDAQKQMDK"
     gene            3936..4394
                     /locus_tag="VHV99_11650"
     CDS             3936..4394
                     /locus_tag="VHV99_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006052942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX3636125.1"
                     /translation="MNTSSRFAFAVHVLALLSLQEGMPLSSEMIAGSVNTNPALIRRL
                     LSMLADAGLTTSQLGAGGGALLARPPDDISLLEVYRAVDDAQLFAMHREEPNPACMVG
                     RNIQVVLRGIIDEAQQAMEASLGARTLADATSDLVRAERARERKRRPQTT"
     gene            4536..5183
                     /locus_tag="VHV99_11655"
     CDS             4536..5183
                     /locus_tag="VHV99_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008924328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX3636126.1"
                     /translation="MSKQLKIALFGATGMIGSRIAAEAARRGHQVTALVRNPARVPAD
                     VANLQAAQADLLDAASVGAAVRGHDVVTSAYAPPQDDPGVVGKATHALVEGVRAAGLK
                     RLVVVGGAGSLEVAPGKQLVEADGFPDAYKPIALAHRDAFDYYRGVTDLDWTFFAPAA
                     LIAPGERTGQFRTGANALLADAEGNSRISAEDYAIAFVDELEQGRFIRQIATVAY"
     gene            5374..7980
                     /locus_tag="VHV99_11660"
     CDS             5374..7980
                     /locus_tag="VHV99_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX3636127.1"
                     /translation="MQSCPSRRESGAAPSNGGQPATHAAFRLSFTHAGCRVRRGVRAH
                     VAACFGVWAATVCLALVLGIGWMPATVHAAGTTTPVIPALQSLINSATATPASGASAA
                     EAAEAASAPSPASQAELARSLDSVIATLDNDRQRTALVAQLKKLREVSQNVAPPASAQ
                     PSPGLLGAIAAGIASFESDVHQGRTPVRYWGGRFNAAGNELYTIISGQGQESLGRIVF
                     SMFAMLAGWGACAGALIYVQHRLYRRFGILMGLKPNPTTRELLIFALRRVGPWIVAFL
                     AALLFVRAMPDALGRTLGMVVAYAIVAGAVFSAICLIMFSLFGSGHRRVAVHLLIEHA
                     RRVLFVVGICAALGDAAVNYDVEHQLGSNLAALISTAANMTAAVLTGYFALAFRRPVA
                     HLIRNRPYEQRHHHKAATDAFDVLAALWHVPVLVLATASVVATLGGSGSSENVLQISV
                     VTALLLVFAFFLSAVVLRMTRPRNPRARRRSPYLTRLLRFCGTLLTLFIWLFFFELAA
                     RLWGVSLAEVVEENVAARGIAHAVTAIIATVFIAWLLWILVDTAITEALNPGAPRNKS
                     RNPSMRARTMLPLLRNVLLVSIMTIAGIVTAANLGINVTPLLAGAGVIGLAIGFGAQS
                     LVTDLITGLFIIIEDTISVGDWIDVDGGHAGTVEHLSIRTVRLRDGQGAIHAIPFSQI
                     KIVKNLSRDFAYAVFEVRMSFSTDVDQITQLIREVGADLMADFRYRREMLGPIEVWGL
                     DRFDPNWMVVKGQIKTRPLQQWSVARAFNLRLKRKMDEAGIEIPVPQMRVYTSSKDSE
                     GQPLQDDELPGAGPHGHGHAHVEAVASTRVAHAAVARDVSHEPRPAPAPTGQTAPIPP
                     QIPTAGDAGGKN"
BASE COUNT         1338 a         2510 c         2709 g         1530 t
ORIGIN      
        1 gcctcgtcac ggtgcatcac cgcaccggga tgggtttcat tgcgattctt gttgatcgaa
       61 tgacgtgtcc ttctttgcac ctcaaaagca tcttaagcac aaatgaggtg catatcgctc
      121 cgaataggtg aacgtgatcc aaatatgggc aaaatattcg atttgcttca cgtgtaattc
      181 aaattgccga cgtttaaccc gcattcgcgg cccgcggggc ggatctggcg gtgaaagcct
      241 gcttcggggc gcgccgtctt tgtcttcctg tacaaacgcc cacataatgt gccgttcccg
      301 catgttctgc cggccctcga cttccaatgc gtacgcatga gactgaccac taaaggcctg
      361 ttgctgatcg cgattccggc cctcttcgaa ctggcgctgc tgtccggcct ggtcaaggcc
      421 caatcagatg cggcgcaagc cgaacagtgg gccatccata gcgaagacgt gctgcgtcag
      481 accaccgcga ttctcgatcc ggtgctaggt gagtccgtcg cgctacgcgg tgcggtgctc
      541 gccaacgata cccgcttcac gacgccggtt accttctgga tggacgtcga ccgtcgcatc
      601 gatcagctgg ccgaactggt tgccgataac ccggcgcaag tcgagcgcgt ggtgcaggtg
      661 cgtcaggagg tgcaggggta tcgtcaatgg tcggaccgcg ttcaggacat gctgcattcc
      721 gggcggcggc gcgatctgct cgagcgcttc cgcgagctgg cgccggccga cgtgctggat
      781 agcttccgtc agcaggtggc agccttccag gccgaagagc ggcatctcga cacgttgcgc
      841 tcgaacgccg ccgacgccgc gcgtgaacgg cagcagatgc tgatcgtcgc ggccgtgttc
      901 ggctccttgc tgtttgtcgc gctggccgtc tggctgttca cgcgcggcgt gcgcggccgg
      961 ctcgcggtgt tgtccgataa cgccgggcgc ctcgcgggca acgagccgct ggccccgctc
     1021 agtccgggcc gtgacgaaat tgcccagctc gatctgacct tgcatgaaac cagccggcgt
     1081 ctgctcgagg ccgagcggat tcaggcgcgt ttccaggccg atctcgcgcg ccgtaccggc
     1141 gaactggcgc gcatcaacga gaccttgcgg caacagaccc aggaaaacga aatgttcatt
     1201 tacagcgtgt cgcacgacct gcgtgcgccg ctggtgaatc tgcaaggctt ttcgaaagaa
     1261 ctgattcggg cgtgcgacga actgcgcgcg atgctgcgcc agtcgtcgct cgcggcccag
     1321 ccgcggcagc gcatcgaacg ggtggtggac gaggatatcg gcgaggcgct gcatttcctg
     1381 cagacggccg tgctgcgtgc ctcgcacatc attgacgcgc tgttgcggct gtcgcgtgtc
     1441 ggccgcgtcg agtatcggca gcaaaaggtc gaagtgcgcg atttcgtgcc gcgcgtggtc
     1501 gacgccatgc aggtgtcgat ccgggcgcgc cgggcgcgtg tgatcgtcga ggacttgccg
     1561 gcggtgtggg gcgacccgac cgcgctcgaa caggtcttcg cgaatctgat cggcaatgcg
     1621 gtcaattatc tggatcccga gcgcgaaggc cggatcgaaa tcggtacgac gccggcgccc
     1681 cccggcgtgc attcgctacg gattttctac gtgcgggaca atggtctggg cattccggcg
     1741 gtcgcgctgc cgcggctttt caacgccttt caacggctgc acggcaatgt ggcggcgggt
     1801 gagggcatcg gccttgcgct cgtgcggcgc gtggtggagc ggcacggcgg gcgcgtatgg
     1861 gcggagtcga aagaaggtat gggcacgacg ttttatctgt cgctgcccga agccgaggcg
     1921 cggatggcgc agctgtctgt tgaaacgcat gccgcgtcgg ccgacggtgt tcatgcggtt
     1981 cgtggtgcgc gcgaagggcg cgccggcctc gctgatcgcg atggggcagg cctgagcggt
     2041 ggtgcggcga cacatgttgc caataccgcc aataccgcta gtgccgccaa taccgctgat
     2101 gccgcgcccg gtatcagtac gcggtaaaga gtcggcgcgg tttgagcagt ttgctgccgc
     2161 gaatcgacca gtagcagcca caggcgatca ggatcgccac gccggtcagc gcctcgagcg
     2221 gcgccgggtg cagcccggcc acgccgtcga gagcgaacag accgcgcgcg gcgatcagga
     2281 gcgccgccca caacgaacag gctgcttgca ccagcatgcg catgcctgcc aaacgtcgcg
     2341 cgcggttttc tcgcgaccag ttggccggtg tgcgtgcgca gaaaaaagca atcgccatca
     2401 acgtgacggc gagaatgacg atccaatcgg tcagttccat tgaaaaggct cccaggtcaa
     2461 gcggcgacta taggaaagcc tttcgctgcg aactatcgga ttagtctcaa aatgcggatg
     2521 cgtttggtgg gtttggcgag caccttgccg caagggcgag gcgcccgccg caggatcaga
     2581 gttcgatccg ggtaccgagc agcacgagga attgacgcag ccactcagga tgagcgggcc
     2641 acgcgggagc ggtgacgaaa ttggcgtcgg tgatggcggc gtcgaccgga atgtcggcgt
     2701 actcgccgcc agccagcttc acttcgggcg cgcaggccgg gtaggcggaa atgcgcttgc
     2761 cgcgaatcac gtcggcggcg gccagcagtt gcgcggcgtg gcagatcgcg gcgatcggct
     2821 tgcccgcttc ggcgaattgc cgcaccagtt cgatcacctt gtgattgagc cgcaggtact
     2881 ccggcgcgcg gccgcctgcg attgccagcg cgtcatactg acggggatcg gcctcgtcga
     2941 atgtggcatt gagggtgaac tgatggccag gcttttcggt gtaggtctgg tcgccttcga
     3001 aatcatggat cgcggtcttg atcttctcgc ccgctttctt gttcggacaa actgcgtcga
     3061 ccacgtggcc gaccgcctgc agtgcctgga acggcaccat cgtttcgtaa tcctcggcga
     3121 agtcgccggt caggaacaga atcttctttg ctgccatcgc atgcctccta ttgatcaacg
     3181 gaacacatcg aacgcttggg aagtctactc caacgcgttg acagaaccgc gacgaggcat
     3241 gtaatgaggc tcgccgcgga gaccacgcgg cgcaacggcg tatcattggc gcgttttcgc
     3301 atgcggaagc cgctcgacgc ggctcacact cagccatgac tttatctatt cgttcaccgt
     3361 tgttgcgccg cgccgccatt gtcgccatcg tgttcggcgg cctcgcggct tgtcagaaga
     3421 acgatgcgtc ggccggacag gtcggcggca agctcgacga tgcggcgcaa gccgccggtc
     3481 agaagctcga tcaggcggcc agctacgtcg gccagcaggt cgacgcgacc aaagccgccg
     3541 cgcagcagaa cctcgaatcg gcctccgcgc cgtcgatcac gatcgatccc gcgtcgctgg
     3601 cgtcgaccgc gcaagccaac ctgcaaggcg cggcaagcgc aaccaatgcg caactcggca
     3661 aggcggctgc gctgaccggc cagagcctcg agtcggcggg gcgcaagctg caggagtggt
     3721 cggcgcaaag ttcggcctcg tcggcgggcg tgtcggcttc cccgtcggcg tcgagcgatt
     3781 caggcgatgc gcaaaagcag atggacaagt gacaggacgt ccgttgtgtc catccgtttg
     3841 agagccggac tggcagccgg tttgacagcc ggtttgacag ccggattaaa tgtaattact
     3901 atggttacac attgtcgttg ccgggcaggg tcgctttgaa tacgagtagc cggtttgcgt
     3961 tcgcggtgca tgtgcttgca ctgctgtcgt tgcaggaggg catgccgctc tcgtcggaga
     4021 tgatcgcggg cagcgtgaat acgaatcccg cgttgatccg gcgtttgttg agcatgctgg
     4081 ccgacgcggg gctcaccacc tcgcagctcg gcgcgggcgg cggcgcgctg cttgccaggc
     4141 cgccagacga catcagcttg ctcgaggttt atcgcgcggt ggacgacgcg caattgttcg
     4201 ccatgcatcg tgaggagccg aatccggcgt gcatggtggg gcggaatatc caggttgtgc
     4261 tgcgcggcat catcgacgaa gcgcagcagg ccatggaggc atcgcttggc gcacgcacgc
     4321 tggccgatgc cacttccgac ctggtgcgcg ccgagcgggc gcgtgagcgc aagcggcgtc
     4381 cgcagacgac gtagcaaaga agcagtaccg aagggcgcgt gtcctgtagt cgagatgcac
     4441 gcgcctgaga acggacgggg gcccacagcc cccctttttt cgacgttata tgtaatcgat
     4501 gcagttacac ataagctaat gacaggagta tcgaaatgag caagcaattg aaaatcgcat
     4561 tgttcggcgc caccggcatg attggttcgc ggatcgccgc ggaagcagcc cgccgcgggc
     4621 accaggtaac cgcgctggta cgtaatccgg cgcgcgtgcc ggccgacgtg gcgaatctgc
     4681 aagccgcgca ggccgatctg ctcgacgccg cgagcgtcgg cgcggcggta cgcggccacg
     4741 acgtggtgac gagcgcatat gcgccgccgc aggacgatcc cggcgtagtg ggcaaggcga
     4801 cccatgcgct cgtcgaaggt gtgcgcgcag cgggcctgaa gcgcctcgtg gtggtgggcg
     4861 gcgcgggttc gctcgaagtg gcgccgggca agcaactggt cgaagcggac gggttcccgg
     4921 acgcctacaa gccgatcgcc ctggcccacc gcgatgcatt cgactactac cgcggcgtca
     4981 ccgatctcga ctggactttc tttgccccgg ccgcgctgat cgcgccgggc gagcgcaccg
     5041 gccagttccg cacgggcgcg aacgcgctcc tcgccgacgc cgagggcaat agccgcattt
     5101 ccgcggaaga ctatgcaatc gcctttgtcg acgaactgga gcaaggccgt ttcatccggc
     5161 agatcgcgac ggtggcttat tgagtttcaa cggcccgtgt gcggtcgtgt cgcgcgtggt
     5221 ccgttgcatg cgtctcagcg gccgttcagc ggataaaggt cgccggtaat tcctcgaaca
     5281 tgcgcaagcg tcctaacaat cggttacgca tgttcggccg ccgccggtaa aactgcggct
     5341 acactggcgt ctcccgtttt ctcacggatc gccatgcagt catgcccgtc gcggcgtgag
     5401 tccggggccg cgccgtccaa cggcgggcaa ccggcgactc acgccgcctt tcgtttatct
     5461 ttcactcatg ccggatgccg cgtccggcgc ggtgttcgcg ctcacgtcgc ggcgtgcttc
     5521 ggcgtgtggg cggccacggt ctgcctcgcg ctggttctcg gcatcggctg gatgcccgcg
     5581 actgttcatg ctgctggcac caccacgccg gtcattccag cattgcagag tctgatcaat
     5641 agcgcgacgg cgacgcctgc ctcgggcgcc tcggcggcgg aagcggcgga agcggcctcc
     5701 gcgccgtcgc ccgcgagcca ggcggaactg gcgcgctcgc tcgacagcgt gatcgccacg
     5761 ctcgacaacg accggcagcg taccgcgctc gtcgcccagc tcaagaaact gcgcgaggtc
     5821 tcgcaaaacg tcgcaccgcc cgcgtccgcg caacctagcc cgggcttgct cggcgcgatc
     5881 gccgcgggca tcgcgtcgtt cgaatccgat gtgcatcagg gccgcacgcc ggtgcgctat
     5941 tggggcggac gcttcaacgc ggccggcaat gagctctaca cgatcatttc gggtcaaggg
     6001 caggagagcc tcggccgcat tgtcttttcg atgtttgcca tgctggccgg ctggggggcg
     6061 tgcgccggcg cgctgattta cgtgcaacac cggctctacc ggcgcttcgg catcctgatg
     6121 gggctcaagc cgaatcccac cacgcgcgag ttgctgatct tcgcgctgcg ccgcgtcggg
     6181 ccgtggatcg tcgcgtttct cgctgcgttg ctgttcgtgc gggcgatgcc cgacgcgctc
     6241 ggccgcacgc tcggcatggt ggtcgcctac gcgattgtcg cgggcgcggt gttttcggcg
     6301 atctgtctga tcatgttttc gctgttcggc tcggggcatc ggcgggttgc ggtgcacctc
     6361 ctgatcgagc acgcgcggcg cgtgctgttc gtggtgggca tatgcgcggc tctcggcgat
     6421 gcggccgtca actacgacgt cgagcatcag ctcggatcga acctcgccgc gctgatttcg
     6481 acggccgcca atatgacggc cgccgtgctc accggctatt tcgcgctggc cttccgccgg
     6541 ccggtcgcgc atctgattcg caaccggcct tacgagcaac gccaccatca caaggccgcg
     6601 accgatgcgt tcgacgtgct cgccgcgctc tggcacgtgc cggtgctggt gctcgccacc
     6661 gcgtcggtgg tcgccacgct cggcggctcg gggtctagcg agaacgtgct gcagatttcg
     6721 gtggtgaccg cgctgttgct ggtgttcgcc ttcttcctct cggccgtcgt gttgcgcatg
     6781 acgcgcccgc gcaacccgcg cgcgcggcgc cgctcgccgt atctgacgcg cctgctgcgc
     6841 ttttgcggca cgctgctgac gctttttatc tggctgttct tcttcgaact ggcagcgcgt
     6901 ttgtgggggg tgtcgctcgc ggaagtcgtg gaggaaaacg ttgcggcgcg cggcatcgcg
     6961 cacgcggtga ccgcgattat cgccacggtg ttcattgcgt ggctgctgtg gattctggtc
     7021 gacaccgcga ttaccgaggc tctcaacccg ggtgctccgc gcaacaagtc gcgcaatccg
     7081 agcatgcgcg cgcgcacgat gctgccgctg ctgcgcaacg tgctgctggt gtcgatcatg
     7141 acgattgccg gcatcgtcac cgcggcgaac ctgggcatca atgtgacgcc gctcctggcc
     7201 ggcgccggcg tgatcggtct cgcgatcggt ttcggcgcgc agtcgctcgt gaccgacctg
     7261 atcacgggcc ttttcatcat catcgaggat acgatttcgg tcggcgactg gatcgacgtg
     7321 gacggcggcc atgcgggcac ggtcgagcat ctgtcgattc gtaccgtgcg cttgcgcgat
     7381 gggcaaggcg cgattcatgc gattccgttc tcgcagatca agatcgtcaa gaacctgtcg
     7441 cgcgacttcg cctatgcggt gttcgaagtg cggatgtcgt tctcaacgga tgtcgatcag
     7501 atcacgcaac tgattcgcga agtgggcgcc gatctgatgg ccgacttccg ctaccggcgc
     7561 gaaatgctcg ggccgatcga ggtgtggggg ctcgaccggt tcgatccgaa ctggatggtc
     7621 gtgaaagggc agatcaagac gcggccgttg cagcaatgga gcgtggcgcg cgccttcaat
     7681 ttgcggctca aacgcaaaat ggacgaagct gggatagaaa ttccggtgcc gcagatgcgt
     7741 gtgtacacgt cgtcgaagga tagcgaagga cagcctttgc aggacgatga gctgcccggg
     7801 gccggtccgc atgggcatgg gcacgcgcat gttgaagcgg tggcatcgac acgggtcgcg
     7861 catgctgcag tggcgcgcga tgtatcgcac gaaccgcgtc cggcgccggc gccgactggg
     7921 caaaccgcgc cgattccccc gcagattccg acggcgggtg acgcgggcgg caaaaactga
     7981 tcgggctggg tggtggttgc tgattgctga ttgcgtcgtt gctggatgct agatgttgat
     8041 tgccagccac cggccaccag ccagcagcta ccgattgcca gtcaccg
//
LOCUS       DATASH010000420         3644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5997662,
            whole genome shotgun sequence.
ACCESSION   DATASH010000420 DATASH010000000
VERSION     DATASH010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5997662"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            339..1343
                     /locus_tag="VHV99_11665"
     CDS             339..1343
                     /locus_tag="VHV99_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX3636128.1"
                     /translation="MTSKLRRLTLSAIAAAILAAPFAAQFSAHADTPLKVGFLVKMPE
                     QAWFINEQKAASALGQKDGFSVVNIGTPDGEKVLAAIDNLGAQGAKGFVICAPDVRLG
                     PAIQARAKRYNMKFVTVDDQLVDSTGKPLPNVPHLGMSAFKIGNQVGTAISEEMKRRG
                     WKPEEVGALRITNYELPTAKLRTDGATESLLAGGFRKENIFDAPQKTTDDEGGFNASS
                     PVLAQHPNIKKWVIFALNEESVLGGVRATEQLHIPAADVIGVGINGAGEAFAEFQKKE
                     PTGFYGTIAVSSTMHGKESTENLVEWIKAGKQPPADTQTTGKLMVRGNWQDVRKELGI
                     "
     gene            1473..3008
                     /gene="araG"
                     /locus_tag="VHV99_11670"
     CDS             1473..3008
                     /gene="araG"
                     /locus_tag="VHV99_11670"
                     /EC_number="7.5.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015612 - ABC-type L-arabinose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042882 - L-arabinose transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HEX3636129.1"
                     /translation="MGQAALPAQAPSLALASADSYLQLDGITVRFPGVLALDQVSLDV
                     RRGEVHGLMGENGAGKSTLLKVLSGVNQPAAGSLALDGVEQQFTTTRAAIAAGVAIIY
                     QELHLVPELTVAENLMLGALPNRFGVLDEKALVARAVRELERLGEKIDPSQQVKNLSI
                     GQRQMIEIGKALMRDARVIAFDEPTSSLSSRETTQLFRIIRALRAEGRAIIYVTHRMD
                     EVYELCDRVTVFRDGRRIKTFEAGEGLDRDRLISCMVGRSIADVYGYRSRELGDVQLD
                     VKELMGRGLREPASFKARKGEILGFFGLVGAGRSELMKLIYGAVKPAAGEITLKGKRV
                     RFATPRDAVRAGLALCPEDRKQEGIVSIASVSDNLNISCRRHFSRFNVLNGRKEAQTA
                     REFIGKLSIKTRNGDTPIGTLSGGNQQKVILSRWLAEDIDVFLMDEPTRGIDVGARSE
                     IYGLLYGLAEAGRTVIVVFSDLAEVIGVADRVIVMKEGRIVGDLPKAQATPDALIKLA
                     LPR"
     gene            3096..>3644
                     /gene="araH"
                     /locus_tag="VHV99_11675"
     CDS             3096..>3644
                     /gene="araH"
                     /locus_tag="VHV99_11675"
                     /EC_number="7.5.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921729.1"
                     /note="Part of the ABC transporter complex AraFGH involved
                     in the high affinity transport of arabinose; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose ABC transporter permease AraH"
                     /protein_id="HEX3636130.1"
                     /translation="MHTPSTDSPAPAIASSGLSARAARTWDLINKSGIVMVFIILFAA
                     LSFTVPDFLSSRNIQGLLLSVTLIGSISVTMMFVLALGEVDLSVASIVAFAGVVASTL
                     ITATHSVTLGIAAGVLAGGAVGLVNGVLVARYKINSLIVTLAMMEVVRGLAYITSNGD
                     AVMISEERFFDLGGGAFLGISYP"
BASE COUNT          699 a         1110 c         1160 g          675 t
ORIGIN      
        1 cacacgtcca gcggcggtcg caaggcgtcc gcctacggcg ttaccgatgc agccttctgg
       61 acggccgtga aaacggctta cgttgcgagt tgatggccgt gggacgcgct caattcgcta
      121 cttcccatac ggctcagtgc gctacgcatg gctgcggcca agccgtcatg tacctcgcaa
      181 gtcactccgc cgcgagcctc gccgccctgc gtagcgcgca ttgcgtggcg cccgctgtcg
      241 cttcgtcgaa cgctcatcca cgtcccggct gacgccgccg cgcattccct gctgcaaata
      301 agcgaaaccc gtgcagcttt tcaactgagg agacaagtat gaccagcaag cttcgccgtt
      361 tgacgctatc cgcgatcgcc gccgcgatcc tcgccgcgcc ctttgccgca caattttccg
      421 cgcacgcgga tacgccgttg aaagtcggct tccttgtgaa gatgccggaa caggcatggt
      481 tcatcaacga acagaaagcc gccagcgcgc tcggccagaa ggacggcttc tcggtggtca
      541 acatcggcac gccggacggt gaaaaagtgc tggccgcaat cgataacctc ggcgcgcaag
      601 gcgccaaagg cttcgtgatc tgcgcgcccg acgtgcggct cggaccggca atccaggcgc
      661 gggcgaagcg ctacaacatg aagttcgtca ccgtcgacga tcagctggtc gactccaccg
      721 gcaagccgct gccgaacgtg ccgcatctgg gcatgtccgc attcaagatc ggcaatcagg
      781 tcggcaccgc gatttctgaa gagatgaagc gccgcggctg gaagccggaa gaagtcggcg
      841 cgctgcgcat cacgaactac gaactgccga ccgccaaact gcgcaccgac ggcgcgacgg
      901 aatcgctgtt ggccggcggc ttcaggaagg aaaacatctt cgacgccccg caaaagacta
      961 ccgatgacga aggcggtttc aatgcatcgt cgccggtact tgcgcagcat ccgaacatca
     1021 agaagtgggt gatcttcgcg ctcaacgagg aaagcgtgct gggcggcgtt cgcgcgaccg
     1081 aacagctgca cattccggcg gcggacgtga tcggcgtcgg catcaacggc gcgggcgaag
     1141 cgttcgccga gttccagaag aaggaaccga cgggcttcta cggcacgatc gccgtgagtt
     1201 cgacgatgca tggcaaggag agcacggaga acctcgtcga gtggatcaag gccggcaagc
     1261 agccgcccgc cgacacgcaa accaccggca agctgatggt gcgcggcaac tggcaggacg
     1321 tgcgtaaaga gctcggcatt taagcggtag tcgagtctgc atgctgcggg ctcttgtcac
     1381 gttcacgttc aagctcctgc cgacgtgaga tcggcccgca caggttgtcc acccgctaga
     1441 ggctttcgat gacggattct cacactggcg aaatgggcca ggcggcgctg cccgcgcaag
     1501 cgccctcgct tgcgctcgcg tcggctgatt cgtatctgca actcgacggc atcaccgtgc
     1561 gctttcccgg cgtgctcgcg ctcgatcagg tgtcgctcga tgtgcggcgc ggcgaagtac
     1621 acggtttgat gggcgaaaac ggcgccggca aatcgacgct gctcaaagtg ctgtccggcg
     1681 tgaatcagcc ggcggcgggc agcttggcgc tcgatggtgt cgagcagcag ttcaccacca
     1741 ccagggcggc gatcgcggca ggcgtcgcga tcatctatca ggaactgcat ctggtgcctg
     1801 agctgacggt tgccgaaaac ctgatgcttg gcgccctgcc caatcgcttt ggcgtgctcg
     1861 atgagaaggc gctggtcgca cgcgcggtgc gcgagctcga aagactcggc gaaaaaatcg
     1921 atccctcgca gcaggtgaag aatctgtcga tcggtcagcg gcaaatgatc gaaatcggca
     1981 aggcattgat gcgcgatgcg cgtgtgatcg cattcgacga accgaccagt tcgttgtcgt
     2041 cgcgcgagac gacgcagctg ttccggatca ttcgggcgtt gagggccgag ggtcgcgcga
     2101 tcatctacgt cacgcatcgg atggatgaag tctatgaact ctgcgaccgc gtgacggtgt
     2161 ttcgcgacgg tcgccgcatc aagacgttcg aggccggtga aggactcgat cgtgatcgtc
     2221 tgatcagctg catggtgggc cgttcgattg ctgacgtgta cggctatcgc tcacgcgaac
     2281 tcggcgacgt gcagctcgac gtcaaggagc tgatgggacg cggcttgcgc gaaccggctt
     2341 cgttcaaagc gcgtaaagga gaaattctcg gcttctttgg cctggttggc gcgggacggt
     2401 ccgagttgat gaaactcatc tacggcgcgg tcaaaccggc tgccggcgag atcacgctca
     2461 aaggcaagcg cgtgcgcttc gctacgccgc gcgatgccgt gcgcgcaggt cttgcactct
     2521 gtcctgaaga tcgcaagcag gaaggcatcg tttcgatcgc ctcggtatcc gacaacctca
     2581 acatcagttg ccgccgccac ttcagccgct tcaacgtgct caacggccgc aaggaagcgc
     2641 agaccgcgag agagttcatt ggcaagctct cgatcaagac gcgcaacggc gacacgccga
     2701 tcggcacgct ctcgggcggc aaccagcaaa aggtgattct gtcgcgctgg ctcgccgaag
     2761 acatcgacgt gttcctgatg gacgaaccca cgcgcggcat cgacgtcggc gcgcgcagcg
     2821 agatttacgg cctgctctat gggttggctg aagcgggccg cacggtcatc gtggtgttta
     2881 gcgatctggc cgaggtgatc ggcgtggcgg atcgggtgat cgtgatgaag gaaggccgca
     2941 ttgtcggcga tctgccgaag gcgcaggcca cgccggatgc gctgatcaag ctcgcgctgc
     3001 ctcgctagga acgccgtgcc cgatcggcca cgtgaaggca agtgaaggta actgaaggca
     3061 attgcatcgc gccattgaaa acggaaatag acatcatgca cacaccctca accgactccc
     3121 ctgcgccagc catcgcgtcc tcgggcttga gcgcgcgcgc cgctcgcacc tgggacctga
     3181 tcaacaagtc cggcatcgtg atggtgttca tcatcctgtt cgcggccttg tcgttcaccg
     3241 tgccggattt tttgagctcg cgaaacatcc agggcttgct gctctccgtc acgctgattg
     3301 gttcgatatc cgtaacgatg atgttcgtgc tcgcactggg cgaggtggat ttgtcggtgg
     3361 cgtcgatcgt tgcgtttgcg ggcgtggttg cttccacctt gatcaccgcg acgcatagcg
     3421 tgacgctcgg catcgccgcc ggcgtgctcg cgggcggcgc agtcgggctg gtcaacggcg
     3481 tgctggtcgc gcgctacaag atcaactcgc tgatcgtcac gctggcaatg atggaagtcg
     3541 tgcgcggcct cgcctacatc acgtcgaatg gcgacgcggt gatgatctcg gaagagcgct
     3601 tcttcgacct tggcgggggc gcgttcctcg gcatttcgta tccg
//
LOCUS       DATASH010000421         3358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1450328,
            whole genome shotgun sequence.
ACCESSION   DATASH010000421 DATASH010000000
VERSION     DATASH010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1450328"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VHV99_11680"
     CDS             <1..406
                     /locus_tag="VHV99_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007740406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3636131.1"
                     /translation="LAGAYVSDTIGRKRAFIAFALASCAIALIYTQLPLDNLTMLVLG
                     FPLGFCASGIFSGMGAFLTELFPTRIRGSGQGFCYNFGRAVGATFPFLIGYVSQTMTL
                     GHAIGGFAAAAYGVVILAALTLPETKGRELNA"
     gene            complement(429..1529)
                     /gene="yjfF"
                     /locus_tag="VHV99_11685"
     CDS             complement(429..1529)
                     /gene="yjfF"
                     /locus_tag="VHV99_11685"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HEX3636132.1"
                     /translation="MRRLLEAWAHIVDPRTLPIAVTILLFCALFGFGSVMYTGFFSWQ
                     VLLDLLVDNAFLLIVAIGMTFVIVSGGIDLSVGSVVALTTIVEAVLSEHLHWPVWLIV
                     PIVLLMGTVFGAVQGALIHFFRLQAFIVTLAGMFFARGLCFLITTQSITINDPTFQKI
                     SAFRLNLGVGSVTANVLIALVTLVAAIYVAHFTRFGRNVYAVGGNPRSALLMGLPVAR
                     TRIGVYALSGFCSALGGAVFTFYVLSGYGLQGQGMELDAIAATVIGGTLLTGGVGYVI
                     GSLFGVGILGTIQALITFDGTLSSWWTRIVIGALLCAFCLLQRLIERHAKSGRRPGGT
                     GAVVTPGREHGRGDTAAAPDSHVIGRAPEQAL"
     gene            complement(1526..2557)
                     /locus_tag="VHV99_11690"
     CDS             complement(1526..2557)
                     /locus_tag="VHV99_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3636133.1"
                     /translation="MKLSNWFSRDGAERPLIWPCVTLVLLCVLNLWVNPHFLSLRMLE
                     GHLFGAPIDVLNRAAPLVLVATGMTLVIATRGIDISVGAVVAIAGAAAATILATQPVP
                     GGGLIAQALVAALIVGVLSGMWNGLLVSFVGMQPIIATLILMVAGRGIAQLLTAGQII
                     PIGAPGYLFVGGGYWLGVPCSVWIASVAVLATAALVEGTALGLFIRAIGVNPVATRLV
                     GLRSKALVFAVYGFSGLTAAMAGILISSNVRSADGNNAGLLLELDAILAVTLGGTSLL
                     GGRFSLTGTVLGALIIQTLTYTTYSIGVPPEATLVVKAAVVLAVSVIQSPSARALAMS
                     LFTQRGVAR"
     gene            complement(2554..>3358)
                     /locus_tag="VHV99_11695"
     CDS             complement(2554..>3358)
                     /locus_tag="VHV99_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3636134.1"
                     /translation="MSARLREAAHDGHTAHEGPQSKQSVQPFVELRGVGRRGTLQPID
                     LDVQPGQILGLAGLLGSGRTETARLLFGADRADSGTILVEGRPVRVRSPRDAVRHGIG
                     YCAEDRKKEGIVAELSIRENILLALQARRGWWRKIKRHRAHEIADMWIERLGIKATDA
                     EQPIGLLSGGNQQKALLARWLATDPRLLILDEPTRGIDVAAKFDIMDRLLALCANGLS
                     ILFISSEISEVLRVSHRVAVLRDRRKIAEVAGKASNEDNIYRLIAGSGE"
BASE COUNT          659 a         1159 c         1027 g          513 t
ORIGIN      
        1 tctcgcgggc gcttacgtca gcgacacaat cggccgcaag cgcgccttca tcgcgttcgc
       61 gctggcctcg tgtgcgatcg cgttgatcta cacgcaactg ccgctcgata acctcaccat
      121 gctcgtgctc ggtttcccgc ttggcttctg cgcgtccggc attttctcgg ggatgggcgc
      181 gtttctcacc gagctgtttc caacccgcat tcgcggctcc ggccagggct tctgctacaa
      241 cttcgggcgg gcggtcggcg cgacgtttcc gttcctgatc ggctacgtct cgcagaccat
      301 gacgctcggc cacgcgatcg gtggattcgc tgccgcggca tacggtgtcg tgatccttgc
      361 cgcgctgacg ttgccggaga ccaaaggacg tgaactcaat gcttgagccg cgtcaccgcc
      421 tgttcgcgct acagcgcctg ctccggtgcg cgtccgatca catgtgaatc gggcgccgct
      481 gcggtgtcgc cacgcccgtg ctcgcggccc ggcgtcacga cagcacccgt gccgccggga
      541 cgccgccccg attttgcatg gcgctcgatc agccgttgca gcaggcagaa cgcgcacagc
      601 aacgcgccga tcacgatgcg ggtccaccac gaactgagcg tgccgtcgaa agtaatcagc
      661 gcctggatcg tgccgagaat gccgacgccg aacagcgaac cgatcacata gccgacaccg
      721 cccgtcagca aggtgccgcc gatcaccgtc gccgcgatcg catcgagttc catgccctgc
      781 ccttgcaggc catagcccga tagcacatag aacgtgaaca ccgcgccacc gagggccgag
      841 cagaacccac tcagcgcata cacgccgatt cgcgtacgcg cgaccggcaa gcccatcagc
      901 aacgccgagc gcggattgcc gccaaccgca tagacattgc ggccaaagcg ggtgaagtgc
      961 gcgacgtaga tcgccgcgac aagcgtgacg agggcgatca acacattggc cgtcaccgaa
     1021 ccgacgccaa ggttgaggcg aaacgcggaa atcttctgga aggtcggatc gttgatggtg
     1081 attgattgcg tcgtgatcag aaagcacaag ccacgcgcga agaacatgcc ggccagcgtg
     1141 acgatgaacg cctgcaaacg gaagaagtga atcagcgcgc cctgtaccgc gccgaacacc
     1201 gtgcccatca gcaacacgat cggcacgatc agccagaccg gccaatgcag atgttcggac
     1261 agcaccgctt cgacgatggt ggtcagcgcc accaccgagc ccaccgacag atcgatcccg
     1321 ccggacacga tcacgaacgt cataccgatc gcgacgatca gcaggaaggc gttgtccacc
     1381 agcagatcga gcagcacttg ccacgagaaa aaaccggtgt acatcaccga gccgaaaccg
     1441 aacagcgcgc aaaacaacag aatggtgacg gcgatcggca gcgtgcgcgg atcgacgata
     1501 tgggcccagg cttcgaggag tcgtctcatc gcgccacccc gcgctgcgtg aagagcgaca
     1561 tggcgagtgc gcgcgccgag ggcgactgga tcacgctcac cgcgagcacc acggccgcct
     1621 tgacgacaag cgtcgcttcc ggcggtacgc cgatcgaata ggtcgtgtag gtgagcgtct
     1681 ggatgatcag cgcgcccagc acggtgcccg tcaggctgaa acggccgccg agcagcgacg
     1741 tgccgccgag cgtcaccgcc agaatcgcat cgagttcgag cagcaggccc gcgttattgc
     1801 catccgcgct gcgcacgttc gagctgatga ggatgcccgc catcgcggcc gtcaatcccg
     1861 agaagccgta caccgcgaat accagcgctt tcgagcgcag gccgacgagg cgcgtcgcca
     1921 ccggattgac gccgatcgcg cggatgaaga gcccgagcgc cgtgccttcg acgagcgccg
     1981 cggtggccag cacggccacg ctcgcgatcc acacggagca cggcacgcca agccaatagc
     2041 cgccgccgac gaacagatag cccggtgcgc cgattggaat gatctgcccc gccgtcaaca
     2101 attgcgcgat accacggccg gcgaccatca ggatcaacgt tgcaatgatc ggctgcatgc
     2161 cgacgaacga caccagcagc ccattccaca tgccgctcaa cacgccgacg atcaacgccg
     2221 caaccagggc ctgcgcgatc aatccgccgc ccggcacggg ttgcgtcgcg agtatcgtgg
     2281 ccgcggcggc cccggcaatc gccacgaccg cgccaaccga aatatcgatg ccgcgtgtcg
     2341 cgatcaccag tgtcatgccg gtggcgacca gcaccagcgg ggccgcgcga ttcaacacgt
     2401 cgatcggcgc gccgaacaga tggccctcga gcatccttaa agaaagaaaa tgcggattca
     2461 cccacaggtt caggacgcac aacagcacca gcgtgacgca cggccagatc agcggacgtt
     2521 ccgcgccatc gcgcgagaac cagttcgata gcttcattcg ccgctccccg cgatgagtcg
     2581 ataaatgttg tcttcgttcg aagccttgcc ggccacttca gcgatcttgc ggcggtcgcg
     2641 cagcaccgcc acgcgatgac tgacgcgcag cacctcgctg atctccgacg agatgaacag
     2701 gatgctcaaa ccgttcgcgc acagcgccag caagcggtcc atgatgtcga acttggcggc
     2761 gacgtcgatg ccgcgggtgg gttcgtcgag aatcagcagc ctgggatcgg tggcaagcca
     2821 gcgcgccagc aacgcctttt gctgattgcc gccggagagc agcccgatcg gctgttcggc
     2881 gtccgtcgcc ttgatgccga ggcgctcgat ccacatgtcc gcgatctcat gcgcgcgatg
     2941 ccgtttgatc ttgcgccacc agccgcgccg cgcctgcaac gccagcagaa tgttctcgcg
     3001 gatcgacagt tcggcgacga tgccctcctt cttgcggtcc tccgcgcagt agccgatgcc
     3061 atgacgcacg gcgtcccgcg gtgaacgcac ccgcacaggc cggccttcga ccaggatggt
     3121 gccgctgtcg gcgcggtccg cgccgaacag caaccgcgcg gtctccgtgc ggcccgagcc
     3181 tagcaggccg gcaaggccca ggatctggcc cggctgcacg tcgaggtcga tcggctgcag
     3241 cgtgccgcgc cgtccgacgc cgcgtagttc gacgaacggc tgaacggact gcttgctctg
     3301 cggtccttcg tgcgcggtgt gcccgtcatg cgccgcttcg cgcaagcgcg cgctcatg
//
LOCUS       DATASH010000422         5811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3031408,
            whole genome shotgun sequence.
ACCESSION   DATASH010000422 DATASH010000000
VERSION     DATASH010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5811
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3031408"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1295
                     /locus_tag="VHV99_11700"
     CDS             <1..1295
                     /locus_tag="VHV99_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012365366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HEX3636135.1"
                     /translation="TDYGGKTQLNLGYLVDSQRQPRGEGFELYTPLWGVLRAGKGLFL
                     SADAQPAANSHALDMSVALEQLQAAQVRMQSLSEAVSKAQAVVAACDAQKALLETQLK
                     DLQQAVLLACAPHGVALTSGEHLQLSAGGHFFTTTGGNADAAIGGNYTVAAGQAVSLF
                     ANTQGMKIYAAAGKVDVQAQGDALSLSALKGVTIASTSDSITLNADKSLTLMCGGAYI
                     KLSDGQVEIGSAGDIKLKGPLRVGPSGTLREALPLMPAQEATGMQLWHAYPNGQPVPN
                     AKYKVTFPDGSWRDGTLDANGRGTLSNAPRGGGTVEYFEEAHDLQDKARRWTDPKGTP
                     RVAGSGSAIGNAADGALPSLAGPAMAAVASVSPIASTVASAASEVASGGVAPLEKAAL
                     GALTHQAMSAANRAAPPSSDVPALPQSLLKPPHRLTAE"
     gene            1495..>5811
                     /locus_tag="VHV99_11705"
     CDS             1495..>5811
                     /locus_tag="VHV99_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012365367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HEX3636136.1"
                     /translation="MSNHSHEIFIQCLYMLPVVGNAMSLYDVGTDIYRLCSVTGAAKN
                     LFNWGILAVDAIGVVPAAGNASRPARAVVKEVLLAFAKGAAVGVLVDLFWASAGGDVI
                     AFMSKLDEKLKGFKQDIVKGVRDASRTVRRFVENPVSAAEQMGAVKKNTGFLSWVPTA
                     EEIALHGIDELLKVSGKRDAILTWLDEFDRNTGPMLDKAFGDAATAGTLIFMAAQIVE
                     EIKARRAHRPSHVAQAAPGTMKEPHTKPGEHRDTTQLGAKPSNLPAKKGCGCPSTASA
                     KPVNYAMGDENLEQTDFALDGVVPIVWTRRYRSSLAAYDDSPLGARWSSPYHLSLELK
                     DGALVFFDPDNRAVPLPLVEVGASVAVPTEQLHVSRADARRVQLAYLDGSREDYERHG
                     TATRERYRLTARISRDGLGLTFDYNDAGELVTISDRAENTIRLEYADGRIASIHRVSA
                     GSRRTETLASYRYSPEGDLIEHQDMLGHQRQFAYQHHLLVRYTDFNGRAANLEWGWPG
                     KRSGQPAPADAKCVRTWLAHNEREVYEDTRFEYHREHWYTKVTDAAGNATFHRYDAHN
                     RIVLVEHGEGTSERFDWDENNNLIGIRNALGQMQRFEYDASGRVIAVTDALGNTTRTE
                     YSAQGLPVTVTSANGDVTRTAYDMLGRPVAVTDAAGRTTQYAWNSAGRVLSFTDPKGG
                     VKQFRYDRAGRLTEAVDCSGYATAYSYDERGYLARRTDAEGNITAYRHDARGQLVQLT
                     RANGVTEQFSWDDEGNLKSYRDGAGQLTEYSFNMRRQPTLRKDAAGHQIVYFHDSHGR
                     LERLRNENDEVTEFRYDALGQLVEQKDVDGRTTRYAYDEAGRLTGSHEGDVKTEFVRD
                     PLGRLTKRDIRAPGMGTYSERFYYDARGRLVTAQSESSLVRLHYDDAGNLIAEEQHQL
                     PTMEAEYVTVTRHEYDALGNRTKTILPNTRTIDWLRYGSGHVHGMLVDGQPLVDFARD
                     KLHRETGRTHSAFSQQREYDPAGRLARFVVRPASAASPHERIAERRLQYSAAGHLTRI
                     EDRTRGVTDYTYDPVGRLLKAVTPDLTEIFAFDPAGNPVDPEKVTPPPVVETPQEKAQ
                     RFARESAEDAAWLRAHPGEKYPPLRHNKRGTEDWAKLYEWRRSLPKCIGNVLAELDRV
                     RYDYDERGNLTRRVERDGRTWLYRYDVANRLSEASRYARTPGADELDRIEPLANGGVS
                     YLNGTARAEQVVRFRYDVFGRRTLKEVERAGGGFDRTVFTWDGDVLLMEERFHEPLRQ
                     TRDVRPYRYRSARLVREDPKDPYSLPVAQRMHTLAEEHQWQAASIYLYEAGSFVPLAR
                     LDETIVEHAHLATGTDGRFVQMPAVTRHATLFYQNDHLGTPLELTDESGKVVWLARYK
                     AWGGWKDTWREKPDPVGGGNALRFAGQYHDDETGLHYNRHRYYDPQTGRFISKDPIGL
                     AGV"
BASE COUNT         1177 a         1754 c         1925 g          955 t
ORIGIN      
        1 cgaccgacta cggcggcaag acacagctca acctgggtta tctggtcgat tcgcagcggc
       61 agccgcgcgg cgaggggttt gaactgtaca cgccgctatg gggcgtgctg cgcgcgggca
      121 agggtctgtt cctcagcgcc gacgcgcagc ctgcggccaa cagccacgca ctcgacatga
      181 gcgtcgcgct ggaacagttg caggcggcgc aggtgcgcat gcagagcttg tccgaagcag
      241 tcagcaaggc ccaagcggtg gtcgctgcgt gcgacgcgca aaaggcgctg ctcgaaaccc
      301 aactcaagga cttgcagcaa gccgtgctgc tcgcctgcgc gccacatggc gtggccctca
      361 ccagcggcga acatctgcaa ctctctgcag gtgggcactt cttcacgaca acgggcggca
      421 atgccgatgc ggctatcggc ggcaactata cggtggcggc gggtcaagcg gtgtcgctgt
      481 tcgctaacac gcagggcatg aagatttacg ccgctgcggg caaggtggat gtgcaggcgc
      541 agggcgatgc gctgtcattg tcggcactga aaggcgtcac gatcgcgagc accagcgatt
      601 caatcacgct gaacgcggac aagtctctca cgctgatgtg tggtggcgcc tatatcaagc
      661 tgtcagacgg acaggtggag attggcagcg cgggcgatat caaactgaaa ggcccgctgc
      721 gggtcgggcc gtccggcacg ctacgcgaag ccctgccgct gatgcccgca caggaggcga
      781 ccggcatgca gttgtggcat gcatacccga acggccagcc ggtgcccaac gcgaaataca
      841 aggtgacgtt tcccgacggc agctggcgcg acggcacgct ggacgcgaac ggtcgcggca
      901 cgctgtccaa tgcgccgcgc ggcggcggca cggtcgaata cttcgaggaa gcgcacgacc
      961 tgcaggacaa ggcgcgcaga tggacggacc ccaagggtac gccgcgggtc gcgggttccg
     1021 gcagtgctat cggcaatgcc gctgacggcg ccctgccgag tctggcgggt cctgcgatgg
     1081 cggctgttgc gtctgtgagc ccgattgcct ccacggttgc cagtgcggca agcgaggtcg
     1141 cttcaggagg cgttgcgccg cttgaaaagg ctgcgttggg cgcgctcacc catcaagcaa
     1201 tgtctgcggc caaccgtgcc gctccaccct cctccgacgt tcccgcgctc ccgcagagcc
     1261 tgctgaagcc gccccatcgg ctgacggctg agtgagcgtt gccctcctat tcaccggatc
     1321 acccaccaga gcatgcccag cgcgaacacc ccgaactcac cgaactcacc gaacacagtt
     1381 gtccgccaga ttccgcagga aatcagtaac aacgccgagg cccggcgcgt catcgccagc
     1441 cccgttccgg cacctgtcac gagacccaac ctcgtgcagg ccggtgttga ctcagtgtcg
     1501 aaccacagcc acgaaatctt tatccagtgc ctgtacatgc tgcccgtggt cggcaatgcc
     1561 atgtcgctgt atgacgtggg caccgacatt taccgcctat gcagcgtgac cggcgcggca
     1621 aagaacctgt tcaactgggg catcctcgcg gtcgatgcga tcggcgtcgt acctgctgcg
     1681 ggcaatgcga gccgtccggc gcgcgcggtg gtcaaggagg tgctgctcgc gtttgccaag
     1741 ggtgctgctg tcggggtgct ggttgacctg ttctgggcga gcgccggcgg cgacgtgatc
     1801 gccttcatgt caaagctcga tgaaaagctg aaggggttta aacaggacat cgtgaaaggc
     1861 gtacgcgacg catcgcgcac cgtacggcgc tttgtggaga atccggtttc cgcagccgaa
     1921 cagatgggcg cggtgaagaa gaacaccgga tttctgtcct gggtgccgac cgctgaggaa
     1981 atcgccctgc acgggatcga cgaactgttg aaggtatcgg gcaagcgcga cgcgatcctg
     2041 acctggcttg acgagttcga ccgcaatacc gggccgatgc tcgacaaggc gttcggcgat
     2101 gccgccacag cgggcacgct gatattcatg gccgcgcaga tcgtcgaaga aatcaaggcg
     2161 cggcgcgcgc acaggccatc gcacgtcgcg caggcggcgc caggcacgat gaaagagcct
     2221 cacacgaaac ccggcgaaca tcgcgacacc acgcagctcg gtgcgaagcc cagcaacctg
     2281 ccggcaaaaa agggctgcgg ctgcccaagt acggccagcg ccaagcccgt gaactacgcg
     2341 atgggcgacg agaatctgga acagacggat ttcgcactcg atggcgtggt gccgatcgtc
     2401 tggacacgcc gctatcgctc gagcctcgcg gcatacgacg acagcccgct gggcgcgcgc
     2461 tggagcagcc cgtatcacct gtcgctggaa ttgaaggacg gcgcgctcgt attcttcgat
     2521 cccgataacc gtgccgtgcc gctgccactc gtcgaagtgg gcgcatccgt tgcagtgcca
     2581 accgagcaat tgcacgtcag ccgcgccgac gcgcggcgcg tgcaactcgc ctatctggac
     2641 ggctcgcgtg aagactatga acggcatggc acggcaacgc gggagcgtta ccgcctgacg
     2701 gcgcgcatct cacgcgacgg tctcgggctt acgttcgact acaacgacgc aggcgaactg
     2761 gtcacgatca gcgaccgcgc ggaaaacacg atccgcctgg agtatgcgga cggccggatc
     2821 gcgtcgatcc atcgcgtgag cgccgggagc cgtcgcacgg aaacgctggc gtcgtacagg
     2881 tacagccccg aaggcgatct gatcgaacat caggacatgc tgggccacca acgccagttt
     2941 gcataccagc atcatttgct ggtgcgctac acggatttca acgggcgcgc cgcgaatctc
     3001 gaatggggct ggccgggcaa acgctcgggc cagccggctc cggccgacgc gaaatgcgta
     3061 cgcacctggc ttgctcacaa cgagcgcgaa gtgtacgagg acacgcgctt cgagtatcac
     3121 cgcgaacact ggtacacaaa ggtcacggac gccgcgggca atgccacgtt ccaccgctac
     3181 gatgcgcaca accgcatcgt gctggtcgaa catggcgagg gcaccagtga gcgtttcgac
     3241 tgggacgaga acaacaatct cattggcatc cgcaatgcgc tggggcagat gcagcgcttc
     3301 gaatatgacg ccagcgggcg cgtgatcgcg gtcactgacg ccctcggcaa tacgacgcgc
     3361 accgaataca gtgcgcaagg attgccggtc acggtcacgt ccgctaacgg cgatgtcacg
     3421 cggaccgcgt atgacatgct cggacggccg gtggcggtga cagatgcggc aggacgcacg
     3481 acgcaatacg cctggaacag tgcgggccgc gtgctgtcgt tcaccgaccc gaaaggcggc
     3541 gtgaagcagt tccggtacga ccgcgccggg cggctcaccg aagccgtcga ttgctctggc
     3601 tatgccacgg cttatagcta cgacgagcgc ggctatctgg cgaggcgcac cgacgccgaa
     3661 ggcaatatca ccgcatacag gcacgacgca cgcggccaac tcgtgcagct cacgcgcgcg
     3721 aatggcgtta cggaacagtt cagttgggac gacgaaggca acctgaagtc gtaccgcgat
     3781 ggcgcggggc aattgaccga atacagcttc aacatgcgcc gccagccgac attgcgcaag
     3841 gatgccgccg gtcaccagat tgtctatttc catgattcgc atgggcggct cgagcggttg
     3901 cgtaacgaaa acgacgaagt cacggaattc cgttacgacg cgctcggcca actggtcgaa
     3961 cagaaggatg tcgacggccg cacgacccgc tatgcctacg atgaggctgg acggcttacc
     4021 ggctcgcacg aaggagacgt gaaaaccgag ttcgtgcgtg acccgctcgg gcggctcacg
     4081 aagcgcgaca tacgcgcacc tgggatggga acctatagcg aacgttttta ctacgacgca
     4141 cgcgggcggc tcgtgaccgc gcagagcgaa tccagcctcg tacgactgca ctacgatgac
     4201 gcgggcaacc tgatcgctga agaacagcat cagcttccca ctatggaagc tgaatacgtg
     4261 actgtcacgc gtcacgaata cgatgcgtta ggcaaccgca ccaaaaccat cctgccgaac
     4321 acacgcacca tcgactggct gcgttacggc tcgggacatg tgcatggcat gctggtcgac
     4381 ggccagccgc tggtcgattt cgcgcgtgac aaactgcacc gcgaaaccgg acgcacgcat
     4441 agcgcattca gccagcagcg cgaatacgat cctgccgggc ggctggcgcg gttcgtcgtc
     4501 aggcctgcga gcgcggccag cccgcacgaa cgaatcgcgg aacgccgttt gcagtacagc
     4561 gcagcgggcc acctgacgcg catcgaggac cgcacgcgcg gcgtgaccga ctacacctat
     4621 gatccggtgg ggcggctgct gaaggccgtc accccggacc tgacggaaat cttcgcgttc
     4681 gatccggcgg gcaatccggt ggacccggag aaagtgacgc cgccgccggt cgtggaaaca
     4741 ccgcaggaaa aagcgcaacg gtttgcgcgc gagtcagcgg aagatgccgc ttggctgcgt
     4801 gcgcatccgg gcgagaaata tccgccactg cggcacaaca agcgggggac cgaggactgg
     4861 gcgaagctgt atgagtggag gcggtcgttg ccgaagtgca tcggcaatgt gctggccgaa
     4921 ctggaccgtg tccgttacga ctacgacgag cggggcaatc tgacacggcg ggttgaacgc
     4981 gacgggcgga cgtggttgta tcggtacgac gtggccaacc gtttatcgga agcgagccgg
     5041 tatgcgagaa caccgggcgc cgacgaactg gatcgcattg aacctcttgc gaatggcggc
     5101 gttagttacc ttaacggcac cgcccgcgcg gaacaggtgg tgcgttttcg ctatgacgtg
     5161 ttcgggcggc gcacgttgaa ggaagttgaa cgggccggtg gcggatttga tcgcacggtg
     5221 tttacgtggg acggcgacgt gctgctgatg gaggaacgtt tccacgagcc gttgcgacaa
     5281 acgcgcgacg ttcgtccgta tcgctatcgc tcggcgcggc tggtgcgtga ggatccgaaa
     5341 gacccgtatt cgttgcctgt cgcgcagcgc atgcatacgc tggctgaaga gcatcaatgg
     5401 caggccgcat cgatttatct gtatgaagcg ggttcgttcg tgccgcttgc gcggcttgat
     5461 gagacgatag ttgaacatgc gcatctggcg actgggaccg acgggcgctt tgtgcagatg
     5521 ccggcggtga cgcgacatgc cacgttgttc tatcagaatg accatttggg cacgccgctg
     5581 gagcttacgg atgagtcggg gaaggtggtg tggcttgcgc ggtataaggc gtggggggga
     5641 tggaaggata cgtggaggga gaagccggac ccggtggggg gtggtaatgc gctgcgcttt
     5701 gcggggcagt atcatgatga tgagacgggg ctgcactaca atcggcatcg gtattatgat
     5761 ccgcagacgg ggcggttcat tagtaaggat ccgattgggt tggcgggtgt g
//
LOCUS       DATASH010000423         6029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4419459,
            whole genome shotgun sequence.
ACCESSION   DATASH010000423 DATASH010000000
VERSION     DATASH010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4419459"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="VHV99_11710"
     CDS             <1..990
                     /locus_tag="VHV99_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636137.1"
                     /translation="LVRNNVNLFPCTLVYVDVDASPGPNSTNRDPLSYVSQAICLNNS
                     LRQVGMPTLTIMTNAPTLVAQRLESVTPERRPALMTLNARIELPKNTPFYAAHFKLDL
                     MDQMADSLPADTMLLLLDADMVALLPLNDDLIERCAEAGLGAFDISDQVFPPYGSERV
                     VADLEIVADRRLRNPRWYGGEFLLATPAALRRLVPCARASYARYITEIERLRHHGDEA
                     FISAALNVLADEGQGLIEVGAYQAVGRHWPGNNYRDVRWFRCCSFVHLPGGKALLEKE
                     ARFVDFVPDRFWRRMRVAHLRGRVRHALKRIVRMLSVERLAALSACATRSVKS"
     gene            complement(1091..2176)
                     /locus_tag="VHV99_11715"
     CDS             complement(1091..2176)
                     /locus_tag="VHV99_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03118 family protein"
                     /protein_id="HEX3636138.1"
                     /translation="MKSVLKVLGGVVSGATLTALVACGGGSGSITTQSFTPTTLVSDG
                     AVSAAHIDANLKNAWGIAFNPKGFVWVADNATSLATLYDGNGVPQPLVVSIPNGINGS
                     ANPTGIVFNGTTDFTVTQGGKTGVGAFIFSGEGGTITAWAPAVAPTTAIVMFDGGSGG
                     AVYKGLALASNGTANFLYATDFHNNRIDVFDKNFAKVAMPGKFQDASLPAGFAPFGIQ
                     AIGAKLFVTYAKQDADAHDNLDGAGLGFVDVFDTSGNLMQHFASAGPLNAPWGIAQAP
                     GNFGRFSGDVLIGNFGDGTINAFDPASGQSLGAVNLPNGTSFVQRGLWGIAFGNGLDN
                     QPVNTLFFAAGPNDEADGVYGRIDVKM"
     gene            complement(2298..3701)
                     /locus_tag="VHV99_11720"
     CDS             complement(2298..3701)
                     /locus_tag="VHV99_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX3636139.1"
                     /translation="MASGKDTAREANGTPAQTKRSTYVEVSSSIESEIRSGVYPPGSR
                     LPPQRQLATELGINVSTVSRAYKELQLRGLVIGSKRRGSLVTGGAMPSVEPAQATTNA
                     AIDLTVNRPATGEFLNCLARTLADLPRDPRYPQLQEYQPPQGPLWARAAGARWMAAPG
                     FTPPPDHVVVTSGAQHGLYAVLNSLIGTDGVILADQLTYYGLKALAPVFQFEIVGIPS
                     DRDGLLTDEVERACRRMPVKAIFTVPNLQNPTVTTMTLARRMALVDIARRHGVAIIED
                     DVYGPLVSQRLPTIASLCPELTFHLAATSKILAPGLRLGYLLSPPDSSALCAEAVRTT
                     AWMPAPMSMLIASIWIEDGTERHIMDAQLAEIRARQDLARELLPQELLQTDPACMFVW
                     LKLPPPWRADDFAANAKARGVVVMPSSAFAVDRSEIEHGVRINLACATSRDQLVSALR
                     LLTGTLKDRPRALFGTI"
     gene            4052..5281
                     /locus_tag="VHV99_11725"
     CDS             4052..5281
                     /locus_tag="VHV99_11725"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006761812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3636140.1"
                     /translation="MPEIAIIGAGFIGLASAAALMRDGHRVTLFDPAGVGQGASFGNA
                     GTFAHYACIPVNNPSVFRDLPRFLLSNQSPFRLRWGYLPHLAPWLARFMMSSLPRRYE
                     ASAGALAALLDRAQEGYAPLLANADLARFVRPRECLYLYSNAASFDAARPALDLRQKL
                     GVAFEVLNGGDIRALEPSLAPIFERGVLFSNSWHFTDPQGFLQTLYQQLAVQGLKLER
                     LAVDAVQPCANGASVTIEGVTRDFSHVVVATGARSAQFAKQCGDLVPLDTERGYHVRF
                     PGAQQLVSRPVGWAERGFYMTPMSDGLRVAGTVELAGFGDARNRSLIDLLTFSSKRAL
                     PALATPDSDWLGFRPTLPDGVPVLGRSRVSERVIYAFGHQHLGLTLAGVSGCIVADLI
                     AQRAPPLDLAPYAATRF"
     gene            5413..>6029
                     /locus_tag="VHV99_11730"
     CDS             5413..>6029
                     /locus_tag="VHV99_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011545635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX3636141.1"
                     /translation="MNRRYWLLSVTVCALVTYIPSSWAVDPEVVKIGFVGPLTGPVAR
                     VGKDLQYGAQLALDEENAKHPVIGGKPVKFVLDVQDDQADPRVAIQVAQKLVDDGVVG
                     VIGHYNSGCSIPASTVYHQANVAMITPGSTNPQLTKQGYKNVFRTMGHDGIGGVVAGH
                     FVVEQMKAKRIGIIDDRTAFGQGLADAFEKGAKEANGNVVDREFT"
BASE COUNT         1074 a         1830 c         1921 g         1204 t
ORIGIN      
        1 cttgtgagga acaacgtgaa tctttttccc tgcacgctgg tttatgtgga tgtcgatgcg
       61 agtccgggtc ccaactcgac gaatcgcgat ccgctctcgt acgtgagtca ggcaatctgc
      121 ctgaacaaca gtttgcggca ggtgggcatg ccgacgctga ccatcatgac caacgcgccg
      181 acgctagtcg cacaacggct cgaaagcgtg acgccggaac gccggcccgc gctgatgacg
      241 ttgaatgcgc ggatcgagtt gccgaagaat acgccgttct acgccgcgca tttcaagctg
      301 gatctgatgg accagatggc tgattcgctg cccgccgaca cgatgctcct gctgctcgac
      361 gccgacatgg tcgcgctgct tccgctcaac gatgatctga tcgagcgctg tgctgaagca
      421 gggctcggtg cgttcgatat ttcggatcag gtatttccgc cgtacggcag cgaacgtgtc
      481 gtggcggacc tcgaaatcgt ggcggaccgg cggctcagaa atccgcgctg gtacggtggc
      541 gagtttcttc tggcgacgcc tgctgcattg cgccggctgg tgccgtgcgc gcgcgccagt
      601 tacgcgcgct atatcaccga aatcgagcgc ttgagacatc atggcgatga agcgttcatt
      661 tcggcggcac tcaatgtact ggccgacgaa gggcagggtc tgatcgaggt gggcgcgtat
      721 caggcggtcg gtcgccattg gcccggcaat aattatcgcg acgtgcgctg gtttcgctgc
      781 tgttcgttcg tccatttgcc aggcggcaag gcgctgctgg aaaaggaggc gcgtttcgtc
      841 gacttcgtgc cggaccggtt ctggcgccgt atgcgcgtgg cgcatttgcg tggccgcgta
      901 cgccatgcgt tgaaacgcat cgtgcggatg ctgagtgtgg agcgtcttgc ggcgctgtct
      961 gcttgcgcta cgcgcagcgt gaagagttag gcgctgatgg cgcggctcgt ggcgagtgcg
     1021 aagccgcgcc gcgtggcggc gcagcgtgag cgcgcgctcg acgctgcacc acgaacgcgc
     1081 gcggcgccgc tcacattttc acgtcgatgc gcccatagac cccgtccgct tcgtcattag
     1141 gccccgcagc aaaaaacagc gtgttgaccg gctgattatc gagcccgttg ccgaacgcga
     1201 taccccaaag cccccgttgt acgaacgagg ttccattcgg cagattgacg gcgccgaggg
     1261 attgcccgct cgccggatcg aaggcgttga tcgtgccgtc gccgaaattg ccgatcagca
     1321 cgtcgccgct gaagcggccg aagttgcccg gcgcctgtgc gatgccccac ggtgcgttca
     1381 gcggaccggc cgacgcgaag tgctgcatca gattgccgga cgtatcgaat acgtcgacga
     1441 agccgagccc ggcaccgtcg agattgtcat gcgcgtctgc gtcctgtttg gcgtaggtca
     1501 cgaaaagttt tgcgccgatc gcctgaatgc cgaacggcgc aaagccggcc ggcagcgaag
     1561 cgtcctgaaa cttgccgggc atcgcgacct tcgcgaagtt cttgtcgaat acgtcgatcc
     1621 tgttgttgtg gaaatcggtg gcatagagaa agttcgccgt gccattgctg gcgagtgcga
     1681 gccccttata gaccgcaccg ccactgccgc cgtcgaacat gacgatcgcg gtcgtgggag
     1741 cgaccgccgg cgcccacgcg gtgatcgtgc cgccttcgcc ggaaaagatg aaggcaccca
     1801 cgccggtctt gccaccctga gtgaccgtga aatccgtcgt gccgttgaac acgatgccgg
     1861 tcggattcgc cgaaccgttg attccattcg gaatgctgac gaccagcggt tgcggtacgc
     1921 cgttgccgtc gtagagtgtc gcgagggagg tcgcgttatc ggcaacccag acgaagccct
     1981 ttggattgaa tgcgatgccc cacgcgtttt tcagattcgc gtcgatgtgt gctgctgaga
     2041 ccgcgccgtc cgagaccaag gtggtcgggg tgaacgactg cgtggtaatt gacccggacc
     2101 cgccgccaca cgcgacgaga gccgtcaacg ttgcgccact cacgacgccg cccaatactt
     2161 taagcacaga cttcatgacc gcacctctct ctgaccgatg gtcgttgcgg ttattgaacg
     2221 tgcggggtgg cgggtttatt ccgttggcga ttgaacaatc tgatttgatc tgcggcgacg
     2281 cccctggtgg gcgtcgatca gatcgtcccg aacaatgccc gaggacgatc cttcagcgtg
     2341 cccgtcagca gtcttaatgc actcacgagt tgatcgcggc tcgtcgcgca cgcgaggttg
     2401 atccgcacgc cgtgctcgat ctccgagcgg tccacggcaa acgccgacga cggcatcacc
     2461 accacgccgc gcgccttcgc attggccgca aagtcgtcgg cacgccacgg cggcggcagc
     2521 ttgagccaca cgaacatgca cgccgggtcg gtttgcagca attcctgcgg caacaactcg
     2581 cgcgccagat cctgccgcgc gcgaatttcg gcgagttgcg cgtccatgat gtggcgctcg
     2641 gtcccgtctt cgatccagat ggacgcgatc agcatcgaca tgggcgcggg catccacgcc
     2701 gtggtgcgca ccgcctccgc acaaagcgcg gaactgtccg gcggactcag cagatagccg
     2761 agccgcaagc cgggggcgag gatcttcgaa gtcgccgcga ggtggaacgt caattccggg
     2821 cacagactgg cgatagtcgg cagccgctgc gaaaccagcg gtccatagac gtcgtcttcg
     2881 atgatcgcca caccatgccg gcgtgcaata tcgacgagcg ccatgcggcg cgccagagtc
     2941 atcgtcgtca cagtcgggtt ctgcagattc ggtaccgtga aaatcgcctt caccggcatg
     3001 cgtcggcagg cacgctccac ctcgtcggtc agcaggccgt cgcggtcgct cggaatgccg
     3061 acgatctcga actggaaaac cggtgcaagg gccttcaggc cgtagtaggt gagctgatcg
     3121 gcgaggatca caccgtccgt gccgatcaga ctgttcagca ccgcatacaa gccatgctgc
     3181 gcaccgctcg tgacaaccac gtgatcgggc ggcggcgtaa agccaggcgc tgccatccag
     3241 cgggcgccgg cggcgcgcgc ccataacggc ccttgcggag gctggtattc ctgcaattgc
     3301 ggataacgcg ggtcgcgcgg caaatccgcc agcgtgcggg cgaggcagtt caggaattcg
     3361 cctgtggccg gccgattgac ggtcaggtcg atcgcggcat tggtcgtggc ttgcgcaggt
     3421 tcgacgctcg gcatcgcgcc gccggttacg agggaaccgc gccgcttgct gccgatcacc
     3481 agcccacgca actgcaattc cttgtaggcg cgggacacgg tcgagacatt gatgccgagt
     3541 tcggtggcaa gttgccgctg cggcggcaag cggctgcccg gtggatagac gccgctgcga
     3601 atctcgctct cgatcgaact ggacacctcg acatacgtcg agcgcttcgt ctgcgccggc
     3661 gtcccgttgg cctcacgcgc cgtgtctttg ccagaagcca taagaaccaa tgtctccata
     3721 caaaatcgac tttgtccgcg attgtgcgga tttttgccga ttctatacca gacatgcgcc
     3781 ggtgcaacgc ttaatcgaga ccgttcggtt tgcttgtgct ctgcggctgc cgggttggca
     3841 aggaccgcgc catggcccat ccagggaaag tgccagggcc acacaatgtg cgttttaatt
     3901 gcacacaaaa aaatattgtg tgattctgat tgcaagcgtt tgtctggtgg cgtgcgtatt
     3961 gaggcgccac tgggccatgt ccagattctg tcaggaggag ttttccgatg agctttccgc
     4021 aaccctggat tccggtggaa gggcgcgagt gatgccggaa atcgccatca tcggtgcggg
     4081 attcatcggc ctcgcgagcg ctgcggcatt gatgcgcgac ggccatcgcg tcacgctgtt
     4141 cgatccggcc ggggtggggc aaggtgcgtc gttcggcaac gccgggacct tcgcgcacta
     4201 tgcctgtatt ccggtcaaca acccgtccgt gttccgcgat ttgccgcgct ttctgctctc
     4261 gaaccagagc ccgttcagat tgcgctgggg gtatctgccc catcttgcac catggcttgc
     4321 gcgtttcatg atgagttcgc tgccgcgccg ttatgaagcg agcgccggcg cactcgccgc
     4381 gttgctcgat cgcgcgcagg aaggttatgc gccattgctt gccaatgcgg acctggcgcg
     4441 tttcgtccgg ccgcgtgagt gcctgtatct gtattcgaac gcggcttcgt tcgatgccgc
     4501 gcgtcctgcg ctcgatctgc gtcaaaagct cggcgtcgcc ttcgaggtgc tcaacggtgg
     4561 cgatatccgc gcgctcgaac cgtcgctggc gccgatcttc gagcgaggcg tgttgttcag
     4621 caacagttgg cacttcaccg atccacaagg ttttctgcag acgctttatc agcagcttgc
     4681 cgtgcaaggt ttaaagctgg aacgtctcgc tgtcgacgcg gtgcagcctt gcgcgaatgg
     4741 cgcgagtgtg acgattgaag gcgttacgcg cgatttcagt cacgtcgtgg tcgctaccgg
     4801 cgcgcgctcc gcccagttcg ccaaacaatg cggggatctc gtcccgctcg atacggagcg
     4861 gggttatcac gtccgctttc cgggcgcgca gcagctggtg tcgcgaccgg tcgggtgggc
     4921 cgagcgcggc ttctacatga cgccgatgag cgatggcttg cgcgtcgccg gcacggtcga
     4981 actggctggc tttggtgacg cgcgcaatcg ttcgctgatc gatctgctga cgttttcttc
     5041 gaaacgcgcc ttacccgcac ttgctacgcc ggatagcgac tggctcggct tccgtccgac
     5101 gctgcccgac ggcgtgccgg tactcggacg atcgcgcgtc agtgagcggg tgatctacgc
     5161 attcggccac cagcatctcg ggttgacgct cgccggggtg agcgggtgca tcgtcgccga
     5221 tctgatcgcg cagcgtgcac cgccgctcga tctggcgcct tacgcggcaa cgcgtttttg
     5281 aatttcgtag aggtttgggc tgcacaggtt gtctcgcttc acgacaaaca tcgcaggacg
     5341 gtttcgacgc gtcgcgcatc ggatcggcca atttatacgt cgcgcgacgt tacatctcgc
     5401 aaggagcgca tcatgaatcg ccgctattgg ctgttgagtg tcaccgtttg cgcactcgtc
     5461 acgtatatac cgtcgtcgtg ggcggtcgat cctgaagtcg tcaagatcgg tttcgtcggg
     5521 ccgctgaccg ggccggttgc acgagtcggc aaagacctgc agtacggcgc ccaactggcg
     5581 ctcgatgagg agaacgcgaa gcatcccgtt atcggcggaa aaccggtcaa attcgtgctc
     5641 gatgtacagg acgatcaggc ggacccccgc gtggcgattc aggtcgcgca gaaactggtc
     5701 gacgacggcg tagtcggtgt gattgggcat tacaactcgg ggtgcagcat tccggcgtcg
     5761 acggtctatc accaggcgaa cgttgcgatg atcacgccgg gctccaccaa tccccaactc
     5821 accaaacaag gctacaaaaa cgtgtttcgc accatggggc acgacggcat tggcggtgtc
     5881 gtagccggcc atttcgtggt ggagcagatg aaggcgaagc gcatcggcat catcgacgac
     5941 cgtaccgcat tcgggcaggg gctcgcggat gcgttcgaaa agggcgcgaa agaagcgaac
     6001 ggcaatgtgg tcgatcgcga attcaccaa
//
LOCUS       DATASH010000424        17907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6395152,
            whole genome shotgun sequence.
ACCESSION   DATASH010000424 DATASH010000000
VERSION     DATASH010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 17907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17907
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6395152"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(264..1514)
                     /locus_tag="VHV99_11735"
     CDS             complement(264..1514)
                     /locus_tag="VHV99_11735"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX3636142.1"
                     /translation="MPKRPTDHPADTASARNSSPASKATRPILPPSPDTPTPLLGNLT
                     PSQFMRRYWQKKPLLIRQAIPNVEAPLSRDEFFELADQDEVEARLITHFRNKWQLEHG
                     PFAPDELPPLKQRAWTLLVQGVDLYDDRARALLDSFRFVPDARLDDLMISYASDGGGV
                     GPHFDSYDVFLLQVKGKRRWRISAQKDLTLEPGLPLKVLQHFEPEEEWLLEPGDMLYL
                     PPHIAHDGVAEGECMTCSIGFRAPSAGELTSQFLYHLAERGESAVRPGALYRDPQQAA
                     VERPAELPAALVERVGAILAQVKWNEQDIASFLGTYLSEPKPSVVFDPPQRPLNEARF
                     ITQASKSGIRIDRKTNLLYNRRFFFVNGEEMPFEGAKKWLFDLADHRCMSAKRFVTLS
                     HDSSVTALLHEWYCAGWIQVGELA"
     gene            1547..2086
                     /locus_tag="VHV99_11740"
     CDS             1547..2086
                     /locus_tag="VHV99_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006025990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX3636143.1"
                     /translation="MKIAKNTVVSVAYKLSDAQGNLIEESDEPMVYLHGGYDGTFPKI
                     EEELNGHEAGFETQIQLEPQDAFGEYDPDLVKIEPRDRFPEPLEVGMQFEGTPEEGDD
                     DLDSLVYVVTDVAEDKVVLDGNHPLAGMALRFALTVKDVRPATEDEIQHEHAHGADGL
                     EVLDEDDEADDDKSGPTLH"
     gene            complement(2115..3521)
                     /locus_tag="VHV99_11745"
     CDS             complement(2115..3521)
                     /locus_tag="VHV99_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636144.1"
                     /translation="MRIIDAHRPSQRSRTLRAALTIALMRTGGFASLVALGLLGLLGL
                     LGSRRFLALRALAALGSFAALAPLAHAETPRYTFAVIANTMQGPADEAPTQRLIEAIS
                     REREVSFIVYDGNLKGAKEACRDALYDRRHALLETSRPALFFIPGQHDWADCGTAEAG
                     GFDPVERLDQLRQTLFTDATSMGQNPIALTRESEVSRFRPFRENVRWQVGDTVFVGLN
                     APSPNNHYLTAGGRNGEFEDRVIANAFWLEHAAEYAKRRDARAIVVFIQGDPDPERYE
                     RPDRFAWLRFTRNTRRDGFLEFKRSLVKLAQIFRGPVLVIHCDDERASAGFVIDQPLR
                     NDKGMLVTNLTRVALAPHDRLNQWIQIEADLGKKPPFRVSVRDVPRQMPMPMAQPVPQ
                     REEPAASMPAVPALGPASELPPLLQTPGQPQPDQQQHRPADDGSEEYGPAMSGPPASG
                     VSAGSAASAVLPRTPGAP"
     gene            complement(3508..6195)
                     /gene="mutS"
                     /locus_tag="VHV99_11750"
     CDS             complement(3508..6195)
                     /gene="mutS"
                     /locus_tag="VHV99_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEX3636145.1"
                     /translation="MGIQTAAANDVAQHTPMMQQYLRIKAEHPGTLVFYRMGDFYELF
                     FEDAEKAARLLDLTLTQRGASAGNPIRMAGVPHHAVEQYLAKLVKLGESVAICEQIGD
                     PATSKGPVERKVVRVVTPGTLTDAALLSDKSDVYLMALCVAHNRRGVATSVGLAWLNL
                     ASGALRLAEAAPDQVAAALERIRPAEILVADTPADSASWAPPVNAGALTRVPVWHFDI
                     TSGTQRLCDQLEVAGLDGFGAHSLTAACGAAGALLLYAAATQGQQLRHVRSLKVEYES
                     EYIGLDPATRRNLELTETLRGTESPTLCSLLDTCCTTMGSRLLRHWLHHPPRAAAVAQ
                     ARQQAIGALLDAPPGAGVETLRGALRQISDIERITGRLALLSARPRDLSSLRDTFIAL
                     PELRTQLAGVSSNADSLARIDASLEPPQACVELLKRAVAQEPSAMVRDGGVIARGHDA
                     ELDELRDISENCGQFLIDLETRERARTGIGNLRVEYNKVHGFYIEVTRGQTDKVPDDY
                     RRRQTLKNAERYITPELKTFEDKALSAQERALARERSLYDALLQALLPFIPDCQRVAA
                     ALAELDLLVAFAERARALDWVAPTFSPDTGIEIEQGRHPVVEAQVEQFIANDCTLTPE
                     RKLLLITGPNMGGKSTFMRQTALIALLAYVGSYVPARRAAFGPIDRIFTRIGAADDLA
                     GGRSTFMVEMTEAAAILNDATPQSLVLMDEIGRGTSTFDGLALAWAIARHLLSHNGCH
                     TLFATHYFELTQLPGEFPHAANVHLSAVEHGHGIVFLHAVSEGPANQSYGLQVAQLAG
                     VPNAVIRAARKHLAHLEQQSAAQPAPQLDLFATPLPMLVEDADDERDARPETAAASPA
                     MQALVERLRAIDPNDLRPREALDLLYELHELAAAPDADH"
     gene            complement(6362..7660)
                     /gene="lplT"
                     /locus_tag="VHV99_11755"
     CDS             complement(6362..7660)
                     /gene="lplT"
                     /locus_tag="VHV99_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HEX3636146.1"
                     /translation="MKKGFFTIIAAQFISSLADNALLIAAIALLSVIRSAAWVTPLLQ
                     IFFTVSYVLLAPFVGAFADALQKRHVMFISNALKASGCLMMICGVHPMIAYGVVGFGA
                     AAYSPAKYGILTELLPAEKLVKANAWLESATVLSTIVGTMVGGALISTLADKFVAHAH
                     LPLIRSSADLAMFAVMVTYAIAAGINIFIPDTGARYPNRLSEPKKLVGDFYHCFNVLW
                     ADKLAQIALWVTTLMWGGAVTLQLLVLKWANVNLGLSLSKAAVMQGVTGLGIAVGASA
                     AAALIPLRSSLRVLPIGILTGAVAIAVAFYNKDLFPAGAGIRIGSYVLPIYILLAYPL
                     MILLGALSGFFIVPMNAILQHRGATLLSAGHSIAVQNFNQNLAVLLMLGAYALLLTAK
                     MPAQWIIVVFGSFITFMMWLAKRRSAVNERTVDMRALVEE"
     gene            complement(7924..8727)
                     /locus_tag="VHV99_11760"
     CDS             complement(7924..8727)
                     /locus_tag="VHV99_11760"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX3636147.1"
                     /translation="MHPMLNIAVKAARRAAQIINRASLDLDLLQVSKKQHNDFVTEVD
                     KASEAAIIDTLKTAYPDHAILAEESGKSANESEYRWIIDPLDGTTNFIHGFPYYCVSI
                     ALAHKGIVTQAVVYDPTRNDLFTASRGRGAYLNDRRIRVAKRDRLADGLIGTGFPFRE
                     TDGLEAYGRQFAEMTEACAGLRRPGAAALDLANVAAGRMDGFFEQGLNPWDVAAGSLL
                     ITEAGGLVGNYTGDSDFLHVGEIVAGNAKVYAQMIPILSRYSRTRQQAA"
     gene            9091..9927
                     /locus_tag="VHV99_11765"
     CDS             9091..9927
                     /locus_tag="VHV99_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX3636148.1"
                     /translation="MDHTHHSSDPAVADLRGGFTSTRFVLVEPSHPGNVGATARALKT
                     MGFSRLVLVSPRMPHVQSDPEAIAMASGADDVLASAHVVPTLADALSGVHWSIALTAR
                     LREYGPPQWTPRAAAGVAREQAAHGEIALVFGNERTGLSNEDVERCSALAHIPANPAY
                     SSLNLAQAVQVLAYELRTAYLSDGEATPAPPAAARAYAAQGEPVGARAASDEIESMYA
                     HLESALVALEFLDPANPKKLMSRLRRLFARSGLEREEVNIVRGIAKHILLKTKPPGGD
                     GS"
     gene            10113..10961
                     /gene="cysE"
                     /locus_tag="VHV99_11770"
     CDS             10113..10961
                     /gene="cysE"
                     /locus_tag="VHV99_11770"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEX3636149.1"
                     /translation="MFTRLREDIATIRERDPAARSAWEVLTCYPGLHALVLHRLAHTC
                     WRAKRRWLARFISQMARFMTGIEIHPGATVGRRVFIDHGMGVVIGETAQIGDDCTIYQ
                     GVTLGGTSLTRGAKRHPTLERGVIVGAGAKVLGGFTIGADAKIGSNAVVTKPVPARGT
                     AVGNPARIIVPAAAAVASATASTALPNATPGGAARDAKRATETSTFCAYGITPNADDP
                     VSLAIHGLIDHAATQSRRIDEIVDALDRLGTSLEGLQGADAALLDLRRLSAAIAGKVE
                     GSAAEH"
     gene            complement(11015..11812)
                     /locus_tag="VHV99_11775"
     CDS             complement(11015..11812)
                     /locus_tag="VHV99_11775"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006763970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEX3636150.1"
                     /translation="MLQETPLRSVAAGVPGEGKRPHAARPFFFLSDIHLSEAIPRTVA
                     AFEHFIRVTAEQADSVFILGDLFEYWIGDDMLVEPFVARMAALLHTLSERGIALYIMH
                     GNRDFLLGKRFMKAAGAIWLPDPFVITAFGTRIVLAHGDGLCTADRGYQIFRRFARSR
                     FAQMLFLAWPFRWRQALAENMRSKSEEGRSRPVSPKYDVTPKGVAALFKTSKTATIIH
                     GHTHRPARHREPGGTRWVLADWDLDHGKRRGGYLRIDAEGIRALPLD"
     gene            complement(11860..12354)
                     /locus_tag="VHV99_11780"
     CDS             complement(11860..12354)
                     /locus_tag="VHV99_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013338826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX3636151.1"
                     /translation="MVELHTNHGVIKLELDAEKAPKSVENFLNYVKAGHYDNTVFHRV
                     IDGFMIQGGGFEPGMKQKPTAEPITNEANNGLKNVNGSVAMARTNDPHSATAQFFINV
                     NDNDFLNHSSPTPQGWGYAVFGKVVEGMDIVEKIKKVKTGSKGFHQDVPADDVVIEKA
                     VIVD"
     gene            complement(12536..13117)
                     /locus_tag="VHV99_11785"
     CDS             complement(12536..13117)
                     /locus_tag="VHV99_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX3636152.1"
                     /translation="MKWLMLALGSAALIANAPAFAQSGSQTAHPSVLFKTTEGDIRVE
                     LYPEKAPKTVANFLDYVKSGQYSGTIFHRVIRGFMIQGGGYTQSFAEKPTRAPIPLES
                     RNGLKNTAGTLAMARTSDPDSATAQFFINTVDNAGLDYPNPDGNGYAVFGKVTSGMDV
                     VKKIEGTPTTTRGPMSDVPQKPIVIESATVVGK"
     gene            complement(13187..13921)
                     /locus_tag="VHV99_11790"
     CDS             complement(13187..13921)
                     /locus_tag="VHV99_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3636153.1"
                     /translation="MKPSSGRARSAATLAATAFCAVALTVLPGAGAYAQKTPTMPQGP
                     AVRDNTPEIDASIAQKNWQTALTQLDARIASNPRDAQARFKRGTVLAHLNRDDEAITA
                     FTELTQMYPELPEPYNNLAALYAKQGRYAEARAALETATKVNPGYGLAYENLGDLYLR
                     MANEAYRHAQSLGKTSATTSQRLIDIQNVISPPKNRTPVKQAAAAPSEASAARASSAD
                     MSQTPSFQFGGANGSFAMPPYMAPSK"
     gene            14177..15574
                     /gene="cysS"
                     /locus_tag="VHV99_11795"
     CDS             14177..15574
                     /gene="cysS"
                     /locus_tag="VHV99_11795"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX3636154.1"
                     /translation="MESLRIYNTLARDKQTFVPLQEGVVRMYVCGMTVYDYCHVGHAR
                     VMVVFDIVQRWLRTLGYDVTYVQNITDIDDKIIRRAVENGETIKSLTDRFITALHEDA
                     DALGIERPDLEPRATDFIPQMLGMIETLEANGYAYQAADGDVNYAVRKFQGYGKLSGK
                     SLEDLRAGERVAANDAKQDPLDFVLWKQAKPDEPADTGWASKYGRGRPGWHIECSAMG
                     CTLLGEHFDIHGGGQDLQFPHHENEIAQSEAATGQTFVNYWMHNGYVQIDNEKMSKSL
                     NNFFTIREVLAQYDAEVVRFFIARAHYRSPLNYSDVHIDDARNALARLYTSLTDVTPE
                     GTALDWNEAHAQRFQAAMNDDFNTPLAVSVLFELATEVNRTRDPALARQLRLLGQVLG
                     LLGREPRAYLQQAAGTAAQGALEPAAIEAKIAARVAAKQAKDYAAADRIRAELLEAGV
                     ALEDKPGGLTEWRRV"
     gene            15622..16698
                     /locus_tag="VHV99_11800"
     CDS             15622..16698
                     /locus_tag="VHV99_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX3636155.1"
                     /translation="MATATKTPAKRATSQTNAAAAAKSTRTSARAGSSAIKNASSKTA
                     GVAAKTGARAAAGATTTAVAGAAKKAGVKRALNGASAHAPVVARAKASRAKSNGVLPA
                     ELAGEVQELARDTHEGEVVRKTRASSSAGGEAASAAELAVPVQVGGLTPEVTRPAYWD
                     KACADLVKRDRILKKLIPKFGPVHLLSRGDPFVTLARSVVGQQISVASAQAVWAKVEA
                     ACPKLVPQQFIKLGQDKLTACGLSRRKAEYVLDLAQHFVSGALHVGKWTSMDDEAVIA
                     ELTQIRGIGRWTAEMFLIFNLSRPDVLPLDDLGLIRAISVNYFSGEPVTRSEAREVAA
                     NWEPWRTVATWYMWRSLDPLPVEY"
     gene            16951..>17907
                     /locus_tag="VHV99_11805"
     CDS             16951..>17907
                     /locus_tag="VHV99_11805"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEX3636156.1"
                     /translation="MKTTFLDFEQPIAELEAKIEELRFVQDDSAVDISEEIERLSKKS
                     QQLTKDLYANLTPWQVSQIARHPQRPYTFDYVNELFTDFHELHGDRSYADDLSIVGGL
                     ARFNGQACMVIGHQKGRDTKERALRNFGMPRPEGYRKAERLMRLAEKFSLPIFTFIDT
                     PGAYPGIGAEERGQSEAIGHNLYVMAELKTPLIATIIGEGGSGGALAIAVGDSVLMLQ
                     FSTYSVISPEGCASILWKSAAKAPEAAEALGLTAHRLKALGWIDKIVNEPLGGAHRDP
                     KGMAAMLRRALADSLRQFQGMSVPDLRQRRFERLMAYGKFKET"
BASE COUNT         3389 a         5408 c         5803 g         3307 t
ORIGIN      
        1 acagggatgc tacgaggagg gatttcttca tgatcacgtc cttttatggt ttaaaggtaa
       61 gcaacagcgc aaaataaaac cggtaatgtg ctccaacacc gacctgggcc gctggtggag
      121 acgctctttt gacgagctgg aatcttccct acggcttggg cggaaattat atgcactttc
      181 gtactgacaa tccataaatc cggggtcaat acgttgtctt tctcatacaa aatttcacag
      241 tacggtataa cacgcgggca gagttacgca agctcgccca cttgtatcca gcccgcacaa
      301 taccactcgt gaagcagtgc tgtcaccgac gaatcgtgtg agagtgttac aaagcgtttc
      361 gcactcatgc accggtgatc ggcgaggtca aacagccatt tttttgcgcc ttcaaatggc
      421 atttcttctc catttacgaa gaaaaaacgg cggttgtaca acaaattagt cttgcggtcg
      481 atgcgaatgc cagacttcga tgcctgagtg atgaaacgcg cttcattgag cggtcgttgc
      541 ggcggatcaa agacgacgct cggcttaggt tcgctcaggt acgtgccaag gaacgacgca
      601 atatcctgct cattccactt gacttgagca aggatcgcgc caacccgttc gactaacgcg
      661 gccggcaact cggctggacg ctcgacggcg gcttgttgtg gatcgcgata gagcgcaccc
      721 ggtcgcacag ccgattcgcc gcgttcggcc agatgataga ggaactgcga ggtcagctcg
      781 cctgcggacg gggcgcgaaa accgatcgag caggtcatgc actcgccttc ggcgacgccg
      841 tcatgggcaa tatgcggcgg caggtacagc atgtcacccg gctcgagcag ccattcctct
      901 tccggttcga agtgctgtaa aactttcaaa ggcaaacccg gttcgagtgt cagatctttc
      961 tgtgcactga tccgccaccg gcgcttgcct tttacttgca aaagaaacac atcgtaggaa
     1021 tcaaagtggg ggccgacgcc accgccatcg cttgcatacg aaatcatcag gtcatccaga
     1081 cgcgcgtctg gcacgaagcg gaagctgtcg agcaaggcgc gcgcgcggtc gtcgtaaagg
     1141 tccacgcctt gcacgagcaa ggtccaggcg cgctgcttga gcggcggcag ttcatcgggc
     1201 gcgaaagggc cgtgctccag ttgccatttg ttgcgaaagt gcgtgatcag acgcgcttcg
     1261 acttcgtcct gatcggcgag ctcgaaaaat tcgtcgcgcg acagaggcgc ctctacgttc
     1321 gggattgcct gccggatcag cagcggcttt ttctgccagt accggcgcat gaattgcgac
     1381 ggtgtcagat tgccgagcaa gggcgtaggc gtgtcgggcg agggcggaag aatagggcga
     1441 gtggcctttg aagccggcga agaattccgt gccgaagctg tatcggccgg gtggtcagtg
     1501 ggccgcttgg gcatcgtata atgtgagttg tattctggag aatcgaatga aaatcgcaaa
     1561 gaacaccgtc gtgtcggtcg cttacaagct atcggatgcg cagggcaatc tgattgaaga
     1621 aagcgacgag ccgatggtct atctgcacgg cggctatgat ggcacgttcc ccaagatcga
     1681 ggaagagctc aacggccacg aggccggctt cgagacccag atccagctcg agccgcaaga
     1741 cgcgttcggc gagtacgacc ccgatctcgt gaagatcgag ccgcgcgatc gcttcccgga
     1801 accgctcgaa gtcggcatgc aattcgaagg cacgccggaa gaaggcgatg acgatctcga
     1861 ttcgctcgtc tatgtggtga ccgacgtggc agaagacaaa gtcgtgctcg acggcaacca
     1921 tccgcttgcc ggcatggcgc tgcgtttcgc gttgaccgtc aaggacgtgc gtcccgctac
     1981 tgaagacgaa atccagcacg aacacgcgca tggcgccgac ggcctcgaag tcctcgatga
     2041 agacgacgaa gccgacgacg acaagtcggg gcccacgctg cactgagctt gcgggcgtgc
     2101 cgggtgtccg tgctttaagg cgcacccggg gtgcgtggca ggacggcgga agcggctgag
     2161 ccggccgaca cacccgaggc aggcggcccg gacatggcgg gtccatattc ctcgctgccg
     2221 tcgtccgccg gcctgtgttg ctgctggtct ggctgcggct gtccgggcgt ctgcaacagc
     2281 ggcggcaatt cggaagcggg acctaaggcg ggcacggccg gcatcgaagc cgccggctct
     2341 tcgcgctgcg gcacaggctg cgccatgggc attggcattt gcctcggcac atcgcgcacg
     2401 ctcacgcgga acggcggttt cttgccgaga tccgcttcaa tctgaatcca ttggttaagg
     2461 cgatcatgcg gcgcaagcgc gacgcgcgtg agattcgtta cgagcatccc cttgtcattg
     2521 cgtagcggtt gatcgatcac aaagccggcg ctcgcgcgtt cgtcgtcgca atgaatcacc
     2581 agcacggggc cgcgaaaaat ctgcgcgagc ttgacgaggc tgcgtttgaa ctccagaaaa
     2641 ccgtcgcggc gcgtgttgcg cgtaaagcgc agccacgcga aacggtcggg tcgttcgtaa
     2701 cgctccggat cggggtcgcc ctgaatgaag acgacaatcg cgcgcgcgtc gcggcgcttc
     2761 gcgtattcgg cggcgtgttc gagccagaac gcgttggcga tcacgcggtc ctcgaactcg
     2821 ccgttgcggc cgcccgcggt cagatagtga ttattcggac tcggagcgtt cagaccgaca
     2881 aacaccgtat cgccaacttg ccagcgcacg ttctcccgga acggacgaaa gcgcgacact
     2941 tcactctcgc gcgtcaacgc gatcggattc tgccccatcg acgtggcgtc ggtgaacagg
     3001 gtctggcgga gttgatcgag ccgctcgacc gggtcgaagc cgccggcctc agccgtgccg
     3061 cagtctgccc agtcgtgctg gcccggaatg aagaaaaggg caggccggga cgtttcgagc
     3121 agcgcgtggc gccgatcgta taacgcatcg cgacaggctt ctttcgcgcc tttcagatta
     3181 ccgtcgtaga cgatgaacga tacttcgcgc tcacggctga tcgcttcgat caggcgttgc
     3241 gtcggcgctt cgtcggcggg gccctgcatc gtgttggcga tgacggcgaa ggtatagcgc
     3301 ggtgtttccg cgtgcgcgag cggggccaac gccgcaaacg acccgagcgc cgcaagcgcc
     3361 cggagcgcca aaaatctgcg cgatccgagt aacccgagta acccgagtaa cccgagcgcg
     3421 acaagcgacg caaagccgcc cgtgcgcatg agggcgatgg tcagcgccgc gcgaagtgtg
     3481 cgcgagcgct gcgagggcct gtgtgcgtca atgatccgca tccggcgcgg cggccagttc
     3541 gtgcagttcg tacagcagat cgagcgcttc gcgcggacgc agatcgttcg gatcgattgc
     3601 gcgcagacgc tcgacaagcg cctgcatggc cggcgacgcc gccgccgttt ccggccgggc
     3661 gtcgcgttcg tcgtcggcat cttcgaccag catgggcagc ggcgtggcga agagatcgag
     3721 ttgcggtgcg ggttgcgccg ccgattgttg ctcgagatgc gcaagatgct tgcgcgccgc
     3781 gcgaatcacc gcattcggca caccggccag ttgcgcgacc tgcaaaccgt agctctgatt
     3841 cgccggacct tcactcaccg catgcaggaa cacgatgcca tgcccatgct cgaccgccga
     3901 cagatgcaca ttggccgcgt ggggaaactc gccgggcaac tgcgtcaatt cgaagtagtg
     3961 cgtggcgaac agcgtatgac agccgttgtg cgacagaaga tgccgcgcga tggcccaagc
     4021 cagcgcgagc ccgtcgaacg tcgacgtgcc ccgcccgatc tcgtccatca gcacgagact
     4081 ttgcggtgtc gcgtcgttca ggatcgccgc cgcctccgtc atctccacca tgaaggtcga
     4141 acggccgccg gccagatcgt cggctgcacc gattcgcgtg aagatgcgat cgatcggacc
     4201 gaatgccgcg cgacgtgccg gtacatagct gccgacatac gccagcaggg cgatcagcgc
     4261 ggtctgccgc atgaaggtcg atttaccgcc catgttcggg ccggtgatca gcagcaactt
     4321 gcgctccggc gtgagcgtgc agtcgttagc gatgaattgc tcgacttgcg cctcgacgac
     4381 cggatgccgc ccctgttcga tttcgatgcc ggtatccgga gaaaacgtcg gcgcgaccca
     4441 atcgagcgcg cgggcgcgtt cggcgaaagc aaccagcaga tcgagttcgg cgagcgccgc
     4501 ggcgactcgc tggcagtccg ggataaaagg caacagcgct tgcagcaacg cgtcataaag
     4561 cgagcgctcg cgggccagcg cgcgttcctg ggcggacagc gccttgtctt cgaaagtctt
     4621 cagttccggc gtgatgtaac gctcggcgtt tttcagcgtc tgacggcggc ggtaatcgtc
     4681 cggcaccttg tcggtctggc cgcgcgtgac ttcgatatag aagccatgca ccttgttgta
     4741 ttcgacacgc agattgccga tgccggtgcg cgcccgttcg cgcgtttcga ggtcgatcag
     4801 gaattgcccg cagttctccg aaatatctcg taactcgtcg agttccgcat catggccacg
     4861 agcgatcaca ccgccgtcgc gcaccatggc ggagggttcc tgcgcaaccg cgcgcttgag
     4921 cagttcgacg cacgcttgcg gcggttccag tgaggcgtcg atgcgcgcca gcgagtccgc
     4981 attggacgaa acgcccgcga gttgcgtgcg caactccggc aacgcgatga acgtatcgcg
     5041 caggctcgac agatcgcgag gccgcgctga cagcagcgcg agacgcccgg taatccgttc
     5101 gatgtcggag atctgccgca atgcgccgcg cagcgtttcg acgcctgcgc ccggcggcgc
     5161 gtcgagcaag gcgccgatgg cctgctgacg tgcttgcgcc acagcggctg cgcgcggagg
     5221 atgatgcagc cagtgacgca gcagacggct gcccattgtc gtgcagcaag tgtcgagcag
     5281 cgagcacagc gtgggcgact cggtgccgcg cagcgtttcc gtgagttcaa gattgcggcg
     5341 cgtggcgggg tcgagcccga tatattcgga ttcgtactcg accttgaggc tgcgcacgtg
     5401 acgcaattgc tggccttggg tggccgcagc atacaggagc agcgcgcccg ccgcgccgca
     5461 tgccgctgtt agcgaatgcg caccgaagcc gtcgagaccg gcgacttcca gctgatcgca
     5521 caaacgctgc gtgcccgagg tgatatcgaa gtgccagacc ggcacacggg tgagggcgcc
     5581 ggcgttgacg gggggcgccc agctcgcgga atcggcgggc gtatcggcca cgagaatttc
     5641 cgcgggacga atccgttcga gcgcggcggc tacctgatcg ggcgcagctt cggcgaggcg
     5701 caacgcgccg ctcgccagat tcagccacgc gaggccgacg ctcgttgcga cgccgcggcg
     5761 gttatgcgca acgcacaggg ccatcaggta cacgtcgctc ttgtcggaca ggagcgctgc
     5821 atccgtcaag gtgcccggcg taaccacgcg caccaccttg cgttcgacgg gccccttcga
     5881 cgtagccgga tcgccgatct gttcgcaaat cgcgaccgat tcgccgagct tcaccagttt
     5941 ggccagatat tgttcaaccg cgtgatgcgg cacgcccgcc atcctgatcg gattacccgc
     6001 cgacgcaccg cgctgcgtaa gggttaaatc gagcaggcgc gcggcttttt ccgcatcttc
     6061 gaaaaagagt tcgtagaagt cgcccatccg atagaacacc agcgtgcccg gatgttccgc
     6121 tttgatgcgc aggtactgct gcatcattgg cgtgtgttgt gcgacgtcat tggccgctgc
     6181 ggtttgaatg cccatccttg gtgtcttttt gcgtttgctg ttcaggccgt gagtttaacc
     6241 tgccggcgtg gcgagcggac cctggccgga cggccaatcc atggcagccc aagctcatct
     6301 cacgcggctt acctgttgcc acgcaaactc ccgcaccagt caggcgacgc tcgccatgcc
     6361 ctcattcttc caccagcgcc cgcatatcca ccgtgcgctc attcacggca ctgcgccgtt
     6421 ttgcgagcca catcatgaac gtgatgaaac tgccgaacac gacgatgatc cattgcgccg
     6481 gcatctttgc cgtcaacagt agcgcatagg cgccgagcat cagcagcacg gccagattct
     6541 ggttgaaatt ctgcaccgcg attgaatgtc ccgcggagag cagtgtcgcg ccacgatgct
     6601 ggagaatcgc gttcatcggc acgatgaaaa agccggacag cgcgccgagc agaatcatca
     6661 gcggataggc gagcaggatg tagatcggca gaacgtatga gccgatccga atgcctgcgc
     6721 ccgctggaaa caggtccttg ttgtagaacg ccaccgcgat cgccaccgcg ccggtgagga
     6781 tgccgatcgg cagcacgcgc agcgaactgc gcaacggaat cagggccgcc gcggcagatg
     6841 cgcccaccgc gatgcctagg cccgtgacgc cttgcatcac cgccgctttc gacagtgaca
     6901 agccgaggtt cacgttcgcc catttgagca ccagcaattg cagcgtgacc gcgccgcccc
     6961 acatcagcgt cgtgacccac agcgcgatct gtgcgagctt gtcggcccac agcacgttga
     7021 agcagtgata gaaatcgccg accagcttct tgggttcgct caaacggttc ggatagcgcg
     7081 caccggtgtc ggggatgaag atgttgatcc ccgccgcgat ggcatacgtg accatcaccg
     7141 cgaacatggc cagatcggcg gacgagcgga tcagcggcaa atgcgcgtgt gcgacgaatt
     7201 tatcggcgag cgtgctgatc agcgcgccac ccaccatcgt gccgacaatc gtcgacagca
     7261 cagtagccga ttcgagccac gcgtttgcct tgacgagttt ttccgccggc aggagctcgg
     7321 tgagaatgcc gtatttggcc ggcgaatacg cggccgcgcc aaagccgacc acgccgtacg
     7381 cgatcatcgg gtggacgccg cagatcatca tcaggcaacc gctcgccttc agtgcgttgg
     7441 agatgaacat cacgtgccgc ttttgcaacg cgtcggcgaa tgcaccgacg aaaggcgcga
     7501 gtaacacgta ggagacggtg aagaaaatct gcagcagcgg cgtgacccag gcggccgagc
     7561 ggatcaccga cagcaatgcg atggcggcga tcagcagtgc attgtccgcg agcgacgata
     7621 tgaactgcgc cgcgatgatt gtgaagaagc cttttttcat gaagcggatc ggaagctgcg
     7681 cggaaatctg gcgcgaagca gggcgctgcg aaaaacgcgc gaaacgcagc cggcgatcgt
     7741 gcgtgccggc gcggcttccc gctttgtagc acgaacgcct gccggatgca ggcgcgccaa
     7801 cgaaaaagcg gccgaagccg cttttcatgt ttctgatgcc ggcgcagagc cggccggagt
     7861 tttgggcctg gacttcagac ctgaggcctg agccccgaga ccgaagccct cagtcccgag
     7921 cccttaagcc gcttgttggc gcgtacggct gtagcgtgac aggatcggga tcatctgcgc
     7981 gtagaccttc gcattgcccg cgacgatttc accgacgtgc aggaagtcgg aatcccccgt
     8041 gtaattgccg accagaccgc cggcctccgt gatcagcagg ctccccgcgg cgacgtccca
     8101 cgggttcagc ccctgctcga agaagccgtc catacggccc gcggcgacgt tggccagatc
     8161 gagcgcggcg gcgcccgggc ggcgcagacc ggcgcaggct tcggtcattt cagcgaactg
     8221 gcggccgtac gcttcgaggc cgtccgtttc gcggaacggg aaaccggtgc cgatcaggcc
     8281 gtcagcgagg cggtcgcgct tggcgacacg gatacgccgg tcgttcaggt acgcgccgcg
     8341 gccgcgcgac gcggtgaaca ggtcgttgcg ggtcggatcg tagaccacgg cctgcgtcac
     8401 gatgcccttg tgggcgagcg cgatcgacac gcagtagtac ggaaaaccgt ggatgaaatt
     8461 ggtggtgcca tcgagcggat cgatgatcca ccgatattcg gactcgttgg ccgacttgcc
     8521 ggattcttcg gcgaggatgg cgtgatcggg gtaggcggtc ttgagcgtat cgatgatcgc
     8581 cgcttcagac gctttatcga cctccgtgac gaaatcgttg tgctgtttct tgctgacctg
     8641 gagaagatcg aggtcgagcg acgcgcggtt gatgatctgg gctgcacggc gcgcggcctt
     8701 cacagcgata ttgagcatgg gatgcatgag actggatcct tgtgccgggg cagcacggct
     8761 gcttaccggt aaatgagcgc gggatgagcg ctaattgggc gccatcaagg ggcgcggaaa
     8821 cagcacggaa cgcacgtccg atcaaagacc ggtgtgcatt ccgccgacgg agacgaattg
     8881 aataagaacg aggcgccgga cttttcgcag gcgaaagcca ggcacgaata tcggtatttt
     8941 acctgagtcc gggcgccgcg acagcgatgg ctgcgcggca ggcgggatga ccgggcgagc
     9001 ggggcgcact gtatgcggtc atcacgcgca attcggagcc ggcgccggtt ggcgctacca
     9061 tacgcgttct ttagcttgcc gaaatccatc gtggaccaca cccaccattc ttccgatccc
     9121 gccgtggcgg acctgcgcgg cggttttacg tcgacgcgct tcgtgctcgt cgagcccagc
     9181 catcccggca atgtcggtgc cacggcgcgc gcgctgaaaa ccatgggttt ttcgcgtctc
     9241 gtgctggtgt cgccacgcat gccgcatgtc cagagcgatc cggaagccat cgccatggcg
     9301 agcggcgccg acgacgtgct ggcgtccgcg cacgtcgtgc cgacgcttgc cgatgcgttg
     9361 agcggggtcc attggtcgat cgcgctgacc gcgcggctgc gcgaatacgg gccgccgcaa
     9421 tggacgccgc gcgccgcggc cggcgtcgcg cgtgagcagg ccgcgcatgg cgagatcgcg
     9481 ctggtgttcg gcaacgagcg cacgggtttg tcgaatgagg acgtcgagcg ctgcagcgcg
     9541 ctcgcgcata ttccggccaa tccggcctac agttcgctca atcttgccca ggcggtgcag
     9601 gtgttggcgt atgaattgcg cacggcgtat ttgtcggatg gcgaggcaac gcccgcgcca
     9661 cctgcagcgg ctcgagctta cgcggcgcag ggcgagccgg tcggcgcgcg ggcggcgagc
     9721 gacgagatcg agagcatgta tgcgcatctg gaaagcgcgc tggttgcgct cgagtttctc
     9781 gatccggcca atccaaaaaa gctgatgtcg cgcctgcggc gtctgttcgc gcgttccggt
     9841 ctggagcgcg aagaggtcaa tatcgtgcgc ggcatcgcca agcatattct gctgaagacc
     9901 aaaccgcctg gcggcgacgg ttcttaagga gcgcctcaac cacgatcttg cgacgcccct
     9961 gccggcagcg tgggcttccg gcaggtttcg cgcaatcgcc ctacaatcgc tcgaaacgtt
    10021 ctaagcaaag ctgccgatct gctatgcggc aagcatccgc catgcgtggt gtttcacgcc
    10081 cgcggtcttt tccccacgac acagttcctg ccatgttcac gagacttcgc gaagacattg
    10141 ccacgatccg cgagcgcgat cccgccgccc gcagcgcctg ggaagtcctc acgtgttatc
    10201 cgggtctgca cgcgctcgtg ctgcaccggc tcgcgcacac atgctggcgg gctaaacgcc
    10261 gttggctcgc gcggttcatc tcgcagatgg cgcgtttcat gaccggcatc gaaatccatc
    10321 cgggcgcgac ggtgggccgc cgcgtattca tcgaccacgg catgggtgtg gtgattggcg
    10381 aaacggcgca aatcggcgac gactgcacga tctaccaggg cgtgacgctc ggcggtacgt
    10441 cgctcacgcg cggtgcgaaa cggcatccca cgcttgagcg cggagtgatt gtcggggcgg
    10501 gagcgaaggt gctcggcggc tttacgatcg gcgcggacgc gaagatcggc tcgaatgcgg
    10561 tggtcacgaa accggtgcca gcacgtggga cagcggtagg taatccggca cggatcatcg
    10621 tcccggcagc ggccgccgtg gcttcagcta ctgcgtccac cgccttgccg aatgcgacgc
    10681 ccggcggcgc cgcgcgcgat gccaaacgcg cgacagaaac cagcacgttc tgcgcttacg
    10741 gcatcacgcc gaacgcggac gatccggtct cgctggcaat tcatggtctg atcgatcacg
    10801 ccgccacgca gtcgaggcgt atcgatgaga tcgtcgacgc actggatcgg ctgggcacaa
    10861 gcctcgaagg actgcaaggc gccgatgcgg cgctgctcga tctgcggcgc ctgtcggccg
    10921 cgattgcagg gaaggtggaa gggtcggcgg cggagcatta atgcaatgcg ccgcgaaagc
    10981 gttgcgatga agcggtaagg cgagcgtcag ccgatcaatc cagcggcaac gcccgaatcc
    11041 cttcggcatc gatccgcaga taaccgccgc gccgcttgcc gtggtcgaga tcccaatccg
    11101 ccagcaccca gcgcgtgccg cctggctcac gatgtcgcgc cgggcgatgc gtgtgcccat
    11161 gaatgatcgt ggccgttttc gacgtcttga atagcgcggc gacgcccttc ggcgtcacgt
    11221 catacttcgg cgaaacggga cgcgagcggc cttcctcgct tttcgaacgc atgttttccg
    11281 ctagcgcctg acgccagcga aacggccagg cgaggaacag catttgcgcg aaacggctgc
    11341 gcgcaaagcg ccggaaaatc tgataaccgc gatccgcggt gcaaagaccg tcgccatgcg
    11401 cgagcacgat acgggtgccg aatgcggtga tcacgaacgg atcgggcaac cagatcgcac
    11461 cggccgcttt catgaagcgc ttgccgagca gaaaatcgcg attaccgtgc atgatgtaga
    11521 gcgcgatacc gcgttccgac aacgtatgca gcaacgccgc catgcgcgcc acgaacggtt
    11581 cgacgagcat gtcgtcgccg atccagtact cgaacagatc gccgagaatg aaaaccgaat
    11641 ccgcctgctc agccgtgacg cgaatgaagt gctcgaacgc ggcgaccgtg cgcgggatcg
    11701 cctcgctcag atgaatatcg gagaggaaaa aaaacgggcg cgcggcgtgc gggcgtttgc
    11761 cctcgccagg cacgcccgcg gcgacgcttc gcagcggcgt ttcttgcagc attgaaggtg
    11821 cctgatgtca gttcgaacgg gtcgacgtcg attggacgct tagtcgacga tcacagcttt
    11881 ttcgatcacg acgtcgtcgg ccggcacgtc ctgatggaag cccttcgaac ccgtcttgac
    11941 cttcttgatc ttctcgacaa tgtccatgcc ttcgacgacc ttgccgaaca cggcgtaacc
    12001 ccagccctgc ggcgtcggcg acgagtggtt caggaagtcg ttgtcgttca cgttgatgaa
    12061 gaattgcgcg gtcgccgaat gcgggtcgtt cgtgcgcgcc atggcgaccg agccgttgac
    12121 gttcttcaga ccgttgttcg cttcgttggt gatcggctcg gcggtcggct tctgcttcat
    12181 gccgggttcg aaaccgccgc cctggatcat gaagccgtcg atcacgcgat ggaacaccgt
    12241 gttgtcgtag tgaccggcct tcacgtagtt gaggaaattc tcaaccgact tcggcgcctt
    12301 ttcagcgtcc agttcgagtt tgatgacgcc gtggttcgta tgcagttcaa ccatgatgga
    12361 atcctttgat gaacgggggt gggtaaaagg cgcggccgct cgtcacttga ggtgcgatga
    12421 ggacgggccg cgccgggggc gtggattgac gcgcttacgt attcctgagc agcttgtttg
    12481 cgtgctgcct ttcgtgtgct ttggtgctga ctttcctgcg cttactttcc ttcgcttact
    12541 tgccgacaac cgttgccgac tcgatcacga ttggcttttg cggcacgtcg ctcatcgggc
    12601 cgcgcgtggt ggtgggcgtg ccttcgatct tcttcacgac gtccatgccg ctcgtgacct
    12661 tgccgaacac cgcgtagccg ttgccgtccg gattcggata gtcgaggccg gcgttgtcca
    12721 ccgtgttgat gaagaactgc gcggtggccg aatccggatc gctggtacgc gccatggcga
    12781 gcgtgcccgc ggtgttcttc agaccgttac ggctttcaag tggaattggc gcgcgcgtgg
    12841 gcttctcagc gaagctctgc gtataaccgc cgccctgaat catgaagccg cgaatcacgc
    12901 gatgaaagat cgtgccgctg tactgcccgg acttcacgta gtcgaggaag ttggcgaccg
    12961 tcttcggcgc cttctcagga tacaactcga cgcggatgtc gccttccgtg gtcttgaaga
    13021 ggacggacgg atgcgcggtc tgcgaaccgg actgggcaaa ggcgggggcg tttgcgatca
    13081 gggcggcgct gccgagcgcc aacatcaacc atttcatgaa gatccctcag ggttggaaaa
    13141 acgtgttgcc gaaaacggtg atggtgttgc ctggtgttac gtggcgttac ttcgacggcg
    13201 ccatgtaggg cggcatggcg aacgagccat ttgcgccgcc gaactggaag ctcggcgttt
    13261 gcgacatgtc ggcggagctg gcgcgtgcgg cgctggcctc gcttggcgcg gcggcggctt
    13321 gcttcactgg cgttctgttc ttgggcggcg agatgacatt ctggatgtca atgaggcgct
    13381 gcgaggtcgt ggcgcttgtc ttgccgaggc tctgcgcgtg gcggtacgct tcgttcgcca
    13441 tgcgcagata cagatcgccg agattctcgt acgcgaggcc gtagcccgga ttcaccttgg
    13501 tcgccgtttc gagcgcggcg cgtgcttcgg cataacggcc ctgcttcgca tagagcgccg
    13561 ccaggttgtt atacggctcg ggcagctcgg ggtacatttg tgtgagttcg gtgaacgcgg
    13621 tgatcgcttc atcgtcgcga ttcagatggg ccagcaccgt gccgcgtttg aacctggctt
    13681 gcgcgtcgcg cggattcgag gcgatgcgcg cgtcgagttg ggtgagcgcg gtttgccagt
    13741 ttttctgcgc aatcgacgca tcgatctccg gcgtgttgtc gcgcacggcg gggccctgcg
    13801 gcatcgtggg ggtcttctgc gcgtacgcgc cagcacccgg caagaccgtg agggcgacgg
    13861 cgcagaatgc cgtcgcggcg agggtcgcgg cgctgcgcgc gcggccgctg gaaggtttca
    13921 taggctcagg tctggatgtt atactccgac ccattctaac aaaaggtctg cgcgttccgt
    13981 cgaaccgcaa actgtctttt cgccactcgt ggtcgtctcc gccatgatcg cagcctgacc
    14041 gccgcaccag gtgcgccggt ttgctgctgt tcaggttgca gtacaggtcg ctcccgctgt
    14101 cagttcgcgg ggcgcctcgt gcatgaaaca gcacgaagca cattgcggat ttctttcgac
    14161 ccacgcaccg ttctctatgg aatcactgcg catctacaac acgctcgcgc gtgacaagca
    14221 aactttcgtg ccgctgcaag agggcgtcgt gcggatgtac gtctgcggga tgaccgtata
    14281 cgactattgt cacgtcggcc atgcgcgcgt gatggtcgtg ttcgacatcg tgcagcggtg
    14341 gttgcgcacg ctcggttacg acgtgaccta cgtgcaaaat atcacggaca ttgatgacaa
    14401 gatcatccgg cgtgccgtgg agaacggcga aacgatcaag tcgctgaccg atcgcttcat
    14461 caccgccttg catgaagatg cggacgcgct cggcatcgag cggcccgatc tggagccgcg
    14521 cgccacggat ttcattccgc agatgctcgg catgatcgag acgttggagg cgaacggcta
    14581 cgcgtatcag gcggccgacg gcgacgtgaa ctacgcggtg cgcaagttcc agggctacgg
    14641 caagctgtcg ggcaagtcgc tggaagatct gcgcgcgggc gaacgcgtcg ccgcgaacga
    14701 cgcgaagcag gacccgctcg acttcgtgtt gtggaagcag gcgaagccgg acgaaccggc
    14761 cgacaccggc tgggcgtcga aatacggccg tggccgtccc ggctggcata tcgaatgctc
    14821 ggccatgggc tgcacgttgc taggcgaaca tttcgacatt catggcggcg gtcaggacct
    14881 gcagtttccg caccatgaga acgagattgc gcaaagcgaa gctgccaccg gacaaacctt
    14941 cgtcaattac tggatgcaca acggctacgt gcagatcgac aatgagaaga tgtcgaagtc
    15001 gttgaacaac ttctttacga tccgcgaagt cctggcgcag tacgatgccg aggtcgtgcg
    15061 tttttttatc gcacgcgcgc attaccgttc gccgttgaat tacagcgacg tgcatatcga
    15121 cgacgcccgc aacgcgctcg cgcgtttgta cacgtcgttg acggatgtca cgccggaggg
    15181 cacggcgctc gactggaacg aagcgcatgc gcagcgtttc caggcagcaa tgaacgacga
    15241 cttcaacacg ccgcttgcag tgtcggtgtt gttcgagttg gcaactgaag tgaaccgcac
    15301 gcgcgacccc gcgctggccc gtcaattgcg cttgctcggg caagtgctcg ggttgcttgg
    15361 ccgcgagccg cgtgcatacc tgcagcaggc ggcgggcacc gccgcgcaag gggcgctcga
    15421 gcccgccgcg atcgaagcga agatcgccgc gcgcgtggcc gccaagcagg cgaaagacta
    15481 tgcggcagct gaccggatcc gggccgaatt gctcgaggcc ggcgttgcac ttgaagacaa
    15541 acccggcggg ttgaccgagt ggcggcgcgt gtgagcgcag ctcgaccatc cctttgatcg
    15601 actcgccagg caggaggcag gatggcaacg gccacgaaga cgccggctaa acgagccacg
    15661 tctcaaacaa acgcggcagc cgcggcaaag tcgacacgaa cgtcggctcg cgcgggtagc
    15721 agcgcgataa agaatgcatc gtcgaaaacc gccggggtgg cggcgaagac tggcgcgcgc
    15781 gcagcggccg gtgcaaccac gacggcagtc gcgggcgcag cgaagaaagc cggggtcaag
    15841 cgcgcgctga acggcgcgtc ggcgcatgcg ccggtggtcg cgcgcgccaa agcatcgcgc
    15901 gcgaagagca atggcgtttt gcctgccgag ctcgccggcg aggtgcagga actcgcgcgc
    15961 gatacgcatg aaggcgaggt cgtacgcaaa acgcgagcgt catcgagtgc gggcggcgaa
    16021 gcagcgagtg ctgctgaact cgcggtgccc gtgcaggtcg gcggcctcac gcctgaagtc
    16081 actcgtccgg cgtattggga caaggcgtgc gcggacctcg tcaagcgcga ccgtattctg
    16141 aagaagctga tcccgaagtt tggcccggtg catctgttaa gccgtggcga tccgttcgtc
    16201 acgcttgcac ggtcggtggt cgggcagcag atttcggtag cgtccgcgca agccgtctgg
    16261 gcgaaagtcg aggcggcgtg tccgaagctc gtgccgcagc agttcatcaa gctcggtcag
    16321 gacaagctga ccgcgtgcgg gctgtccagg cgcaaggccg aatacgtgct cgatctcgcg
    16381 cagcatttcg tgtcgggcgc gttgcacgtc ggcaagtgga cttcgatgga cgacgaggcg
    16441 gtgatcgccg aactcacgca gatccgcggc atcggccgct ggactgcgga aatgttcctg
    16501 atcttcaatc tgtcgcgccc cgacgtgctg ccgctcgacg acctcggcct gatccgcgcg
    16561 atcagcgtca actactttag cggtgagccg gttacgcgca gtgaagcgcg tgaggttgct
    16621 gcaaactggg aaccgtggcg taccgtcgcg acctggtata tgtggcgtag tctcgatccg
    16681 ttgccggtcg agtactgagt gtcccaattg gcgcgtcgcg ccaggccctg agggagcaat
    16741 cccccaattg cccgagagga cttcctccgg ggcggagact tccagcgggg cgaaacactg
    16801 ggatgcgggc cggcgttgtc ttgagaagaa caggcagcca tgttgtttca aaagctatca
    16861 atggctaaaa attgatagtt gcgagatggt tttgacagcg gcgagcgcgg ttagaatacg
    16921 cgctgccggt aagtccaagg attacaacca atgaagacca cctttctgga tttcgaacag
    16981 ccgatcgctg aactcgaagc gaagatcgaa gaattgcgct tcgtgcagga cgattcggcc
    17041 gtcgatattt cggaagagat cgagcggctg tccaagaaga gtcaacagct caccaaagat
    17101 ctgtacgcaa acctgacgcc gtggcaggtt tcgcaaattg cccgccatcc gcagcgcccg
    17161 tacacgttcg actacgtgaa cgagctgttc accgatttcc acgaactgca cggcgaccgc
    17221 agctatgcgg acgacctgtc gatcgtcggt ggcctcgcgc gtttcaatgg ccaggcctgc
    17281 atggtgatcg gtcatcagaa gggccgcgac acgaaggagc gcgcgctgcg caacttcggc
    17341 atgccacgtc cggaaggcta tcgcaaggcc gagcgtctga tgcgtctcgc cgaaaaattc
    17401 agcctgccga tcttcacgtt catcgacacg ccgggcgcgt atccgggcat cggcgcggaa
    17461 gagcgcggcc agtccgaagc gatcggccac aatctgtacg tgatggccga actgaagacg
    17521 ccgctgatcg caacgatcat cggtgagggc ggttcgggcg gtgcgctcgc gatcgctgtc
    17581 ggcgatagcg tgctgatgct gcagttctcc acctactcgg tgatttcgcc ggaaggctgc
    17641 gcgtcgatcc tgtggaagag cgcggcgaaa gcgccggaag ccgcggaagc gctcggcctg
    17701 accgcgcatc gtctgaaggc gctcggctgg atcgacaaga tcgtcaacga gccacttggc
    17761 ggcgcgcatc gcgatccgaa gggcatggcc gcgatgttgc gccgtgcgct cgccgattcg
    17821 ctgcgccagt tccagggtat gagcgtgcct gacttgcgcc aacgtcgttt cgaacgtctg
    17881 atggcctacg gcaagttcaa ggaaacg
//
LOCUS       DATASH010000425         5327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5803135,
            whole genome shotgun sequence.
ACCESSION   DATASH010000425 DATASH010000000
VERSION     DATASH010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5803135"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..1409
                     /locus_tag="VHV99_11810"
     CDS             168..1409
                     /locus_tag="VHV99_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3636157.1"
                     /translation="MSSTQLRVFLLFSAGYFVSYVFRGVNLGFAPFITHDLGLSATDL
                     GVLTSLYFLGFAGAQIPAGVLLDHFGARRVTAATLLFAALGIWVFGTAHSVAGMMVGR
                     LLIGVGVSVCLGGAFKALAQHFASARLPLMNGLVMAIGGFGGVMVGSPLTWVLGFTSW
                     RTVCIGLGLLTVLVAVAQWTLAPDIKETRHQASLGAQFKGTLHILSSAAFWKIASFSV
                     VTQGVFYAMQSLWVGAWLRDVQGFEPREAAALVSILGFAMMAGCVGFGAAARSLERRG
                     ISLYVFCGIGMALFVVTQLLIMFSVPLPASLLWTAYGVFGGTGILSYAVMARHFPAQM
                     IGRVNTTLTLIIFLLIFGFQIGVGAVLSHWTASGGKYPAAAHLTAWGILVTLQVLSAI
                     WYVLPGRTLVRPAQVHAEQEA"
     gene            1889..3043
                     /gene="carA"
                     /locus_tag="VHV99_11815"
     CDS             1889..3043
                     /gene="carA"
                     /locus_tag="VHV99_11815"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEX3636158.1"
                     /translation="MLPSFSPALLALADGTVFRGYSIGAPGHTIGEVVFNTAITGYQE
                     ILTYPSYARQIVTLTYPHIGNVGVNAEDVEATKVHAAGLIIRDLPVLASNFRMERTLP
                     QYLKDEGVVAIAGLDTRMLTRVLRDKGAQNGAILAGSDDEAKAIELARSFPGLSGMDL
                     AKVVSTKESYEWTQTEWRLGTGYGTQNAPKYRVVAFDYGVKYNILRMLAERGCHVTVL
                     PAEASAADALALNPDGVFLSNGPGDPEPCDYAIRATKELIERGIPTFGICLGHQIMGL
                     ALGAKTLKMKTGHHGANHPVKDLEDGRVVITSQNHGFAVDADTLPANAKVTHISLFDG
                     TLQGFALTDKPAFCFQGHPEASPGPHDIAYLFDRFTALMDTKKAGKTAAA"
     gene            3215..5327
                     /gene="carB"
                     /locus_tag="VHV99_11820"
                     /pseudo
     CDS             3215..5327
                     /gene="carB"
                     /locus_tag="VHV99_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298333.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
BASE COUNT         1010 a         1624 c         1672 g         1021 t
ORIGIN      
        1 gttgcggggg cagcgcatga attgcgctcc gatcacgcac cgtgttattc caacgccgtc
       61 acccatggtg acggcgtttt tcattctgca gatcaattcg ggcgaagttc gcccgtttca
      121 tttatctttc gtgcttgggc caaatccggc cgctcatctc aaagattatg tcgtcaaccc
      181 agttgcgcgt ttttctcctg ttttccgccg gttactttgt ttcttacgta tttcgcggcg
      241 tcaatctcgg cttcgcacct ttcattacgc acgatctcgg cttgtcggcc acggatctcg
      301 gcgtgctgac cagtctgtat tttctcggct tcgcgggcgc gcagatcccg gccggcgtgt
      361 tgctcgacca cttcggcgca cgtcgggtga cggcggccac cttgctattc gccgcgctgg
      421 gaatctgggt gttcggcacc gcgcacagtg tcgccggcat gatggtcggg cgtctcctga
      481 tcggcgttgg tgtgtccgta tgtctcggcg gcgcgttcaa ggcgctggcg cagcatttcg
      541 cgtccgcacg gctgccgttg atgaacgggc tggtgatggc gatcggcggt ttcggcggcg
      601 tgatggtcgg ctcgccgctc acctgggtgc tgggctttac cagctggcgc acggtctgta
      661 tcggtctggg tctgctgacg gttctggtgg cggtggcgca gtggactctc gccccggata
      721 tcaaggaaac ccgtcaccag gcaagtcttg gcgcccagtt caaaggcact ctgcacattc
      781 tgagcagcgc ggcattctgg aagatcgcgt cgttctcggt cgtcacccaa ggcgtgttct
      841 acgcgatgca atcgttgtgg gtgggtgcct ggttgcgcga cgtacaaggc ttcgagccgc
      901 gtgaagcggc cgcactggtg tcgattctgg gcttcgccat gatggccggc tgcgtgggtt
      961 tcggcgcggc ggcacgcagc ctcgagcggc gcggcatctc gctgtacgtg ttttgcggca
     1021 ttggcatggc gctgttcgtt gtcacccagc tgctgatcat gttcagtgtg ccgctgcccg
     1081 cgagcctgct gtggacggcg tacggcgttt tcggcggcac cggcattctc agctacgcgg
     1141 tgatggcgcg gcacttccca gcgcagatga tcgggcgggt gaataccacg ctcacgctga
     1201 tcatttttct gctgattttc ggtttccaga tcggtgtcgg cgcggtgctg tcgcactgga
     1261 cggccagcgg cggaaaatat ccggcggccg cgcatctgac cgcctggggc attctggtga
     1321 cgctgcaggt attgagcgca atctggtatg tgttgcccgg ccgcacgttg gttcggccgg
     1381 cgcaggtgca cgctgagcag gaggcctgag gcggtgatgc tgtttcggcc gtcgcaggta
     1441 gacgctgaat tgcagacctg aggcggtcgt gcgcggagct tggggcggac tgtcgtattt
     1501 accgacccta tcccgattct ggtccgtgtc gatattgtcc gatggcttcg tgccaggcgc
     1561 ggcaaggctt tgaacgggtt cgggtcgcgc ttcgccgggt cgccgcgcca tctcggattt
     1621 ggcgggaagg gtgatttcac gctataattt caaggtttga gcttatcaac ccgcacccta
     1681 gcgcctacct ctcccaaacc gttcatccgc cgcgcgcttt tgacgccccg cacccagctg
     1741 attcgtccac acaatggacc tatcgcgccc ctgcgacgtc accagcagta cgcgagtgag
     1801 tctgcgcgcg catcctgtcg atgcgtttcg ctgcggctcg cagtcggata gacaggtcgg
     1861 caacagggtg ttccccaagg agtctcccgt gttgccgtca ttttctcccg ctttgctcgc
     1921 actcgccgac ggcacggtct ttcgtggtta ctcgatcggc gcccccgggc atacgatcgg
     1981 cgaagtcgtg tttaacaccg ctatcaccgg ctatcaggaa atcctgacct acccgagcta
     2041 cgcgcgtcag atcgtgaccc tcacgtatcc gcacatcggt aacgtcggcg tgaatgccga
     2101 agacgtcgaa gccacgaaag tccatgccgc cggcctgatc atccgtgatc tgccggttct
     2161 cgcgtcgaac ttccgcatgg agcgcacgct cccgcaatac ctcaaggacg aaggcgtggt
     2221 cgccatcgcc ggtctcgaca cccgtatgct gacccgtgtg ctgcgcgaca agggtgcgca
     2281 gaacggcgcc attctggccg gctcggatga cgaagcgaag gcaatcgaac tcgcgcgctc
     2341 gttcccgggt ttgtcgggta tggacctcgc gaaagtcgtg tcgacgaaag aaagctacga
     2401 atggacccag accgaatggc gtctgggcac cggctacggc acgcagaacg cgccgaagta
     2461 ccgcgtggtc gcgtttgatt acggcgtgaa gtacaacatc ctgcgcatgc tggctgaacg
     2521 cggctgccac gttaccgtgc tgccggcaga agcttcagcg gctgacgcgc tggcgctcaa
     2581 cccggacggt gttttcctgt cgaacggccc gggcgatccg gaaccgtgcg actacgcgat
     2641 tcgcgccacc aaagagctga ttgaacgcgg cattccgacc ttcggcatct gcctcggcca
     2701 ccagatcatg ggccttgcgc tcggcgccaa gaccctcaag atgaagaccg gccaccacgg
     2761 cgcgaaccat ccggtgaagg atctcgaaga cggccgcgtg gtcatcacgt cgcagaatca
     2821 cggcttcgcg gtcgacgccg acacgctgcc ggccaatgcc aaggtcacgc acatctcgct
     2881 gttcgacggc acgctgcaag gcttcgcgtt gaccgacaag ccggcgttct gcttccaggg
     2941 ccacccggaa gcgtcgcctg gcccgcacga catcgcctat ctgttcgacc gcttcactgc
     3001 gttgatggac acaaagaagg ccggcaagac cgcagcggca taagggcgtt tgaagccata
     3061 gcttgtgaag cgccctggcg gcgcgaggct cgccccgcaa tccgcgcact cagcgcgaag
     3121 cgcggcgagc cgcacaacac agagcagagc gcgcgcaacg taacaacgca gcgcgccgac
     3181 ggaaagaatt caggaataca ttagcgagag cgttatgccg aagcggacag acatcaagag
     3241 catcctcatc atcggcgcgg gtccgatcat catcggccag gcgtgcgagt tcgactactc
     3301 gggcgcgcag gcatgcaagg cgttgcgtga ggaaggctac aaggtcattc tcgtcaacag
     3361 caatccggcg acgatcatga ccgacccgaa cacggccgac gtgacctaca tcgagccgat
     3421 cacgtgggaa gtggttgaac gcatcatcgc caaggagcgc ccggacgcga tcctgccgac
     3481 gatgggcggc cagaccgcgc tgaactgcgc gctggatctc cacgcacacg gcgtgctgga
     3541 caagtacaag gtcgagctga tcggcgcgtc gccggaagcg atcgacaagg cggaagaccg
     3601 ccagaagttc aaagacgcga tgacgaagat cggcctcggt tcggccaagt cgggcaccgc
     3661 gcattcgatg gaagaagcac tgcaggtgca ggccgacatt gctgtgcaaa caggcagcgg
     3721 cggttatccg gtcgtgatcc gtccgtcgtt cacgttgggc ggctccggtg gcggcatcgc
     3781 gtacaaccgc gacgaattcg aagagatctg caagcgcggt ctggacctgt cgcccacgcg
     3841 cgaactgctg atcgaagagt cgctgctcgg ctggaaatag tacgagatgg aagtggtccg
     3901 cgacaaacag gacaactgca tcattgtctg ctcgatcgaa aacctggacc cgatgggcat
     3961 ccacaccggc gactcgatca ccgtcgcgcc ggcgcaaacg ctcacggaca aggaatacca
     4021 gatcctgcgt aacgcatcgc tcgccgtgct gcgcgagatc ggcgtggaca cgggcggttc
     4081 gaacgtgcag ttctcgatca atccgaaaga cggccggatg atcgtgatcg aaatgaatcc
     4141 gcgtgtgtca cgttcgtcgg cgttggcgtc gaaggctacc ggcttcccga tcgcgaaggt
     4201 cgcggcaaag ctggcagtcg gctacacgct ggacgaactg aagaacgaaa tcaccggcgg
     4261 tcagaccccg gcctcgttcg aaccgactat cgactacgtc gtcaccaaga tcccgcgctt
     4321 cgcgttcgag aaattccgcg aagccgattc gcgcctgacc acgcagatga agtcggtcgg
     4381 tgaagtgatg gcgattggcc gcaccttcca ggaatcgttc cagaaagcgc tgcgcggtct
     4441 ggaagtgggc gtcgacggtc tggacgaaaa gaccgacaac cgcgacgaga tcatccgcga
     4501 gatcggcgaa gccggcccgg atcgcatctg gtacgtgggc gacgcattcc gtgtcggcat
     4561 gacggccgaa gagattttcg aagagacgtc gatcgacccg tggttcctcg cgcagatcga
     4621 acagatcatc ctgaaggaaa aggcgttggc cggccgtacg ctggcaagcc tgtcgaagga
     4681 agagctcaag tatctgaagc aaagcggctt ctcggatcgt cgtttggcga agctgctcgg
     4741 cgcgaagccg gcggaagtgc gtcaacgccg tatcgagttg aatgtgcgcc cggtctacaa
     4801 gcgcgtcgac acgtgcgcgg ccgagttcgc cacgaaaacc gcctacatgt actcgaccta
     4861 cgaggaagag tgcgaagcga acccgaccaa caacaagaag atcatggtgc tgggcggcgg
     4921 cccgaaccgg atcggccagg gtatcgagtt cgactactgc tgcgtgcatg ccgcgctcgc
     4981 catgcgcgaa gacggctacg aaacgatcat ggtcaactgc aaccctgaaa ccgtttcgac
     5041 cgactacgac acgtccgatc gtctgtactt cgaatcgctg acgctcgaag atgttctgga
     5101 aatcgtcgac aaggaaaagc cggtcggcgt gattgtgcaa tacggcggtc aaacgccgct
     5161 gaagctcgcg ctcgatctcg aagcgaacgg tgtgccgatc atcggcacgt cgccggacat
     5221 gatcgacgcc gccgaagacc gcgagcgttt ccagaagctg ctgcaagacc tcgggctgcg
     5281 tcagccgccg aaccgcaccg cgcgtgccga agacgaagcg ctcaggc
//
LOCUS       DATASH010000426         5088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6790779,
            whole genome shotgun sequence.
ACCESSION   DATASH010000426 DATASH010000000
VERSION     DATASH010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5088
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6790779"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="VHV99_11825"
     CDS             complement(<1..724)
                     /locus_tag="VHV99_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX3636159.1"
                     /translation="MTRTIRKVFVANRGEIAVRIIRAARELGLQTVLGCSEADTDSLA
                     ARMADSIEVLGPAPAAKSYLDTRAVVEAARRSGADAVHPGYGFLSENAAFAQVLKDAG
                     LIFVGPSPETISLMGDKAAARACARRAGVPTVPGSDGVVEHVEEAQREALRVGYPVML
                     KAAAGGGGRGIRVAQDADELAAEFDKATREAESAFGYGGLYLERFVGRAHHVEVQIMG
                     DGVRAIHLYDRECSLQRRRQKLM"
     gene            complement(726..977)
                     /locus_tag="VHV99_11830"
     CDS             complement(726..977)
                     /locus_tag="VHV99_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HEX3636160.1"
                     /translation="MADFEVKSEIPGTFYRSSAPGKPPLVEEGQSVSAETIIGIVELM
                     KQFNEVLAGVAGVVRSFAVADGDAVDADAVLVVIDTGSV"
     gene            complement(1008..2012)
                     /locus_tag="VHV99_11835"
     CDS             complement(1008..2012)
                     /locus_tag="VHV99_11835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HEX3636161.1"
                     /translation="MLDIVKPGLETTVQELPGRIGHWEQGFPPSGPADAWSFRLANLL
                     VGNDRDTAALEWQFVGPTVRFHRDGAIALTGADMAAKLDDTPVPMWETLNVRAGQVLT
                     CGAARTGARGYIAVSGGITTEPVLGSRATFHMAAIGGTGNGALKKDDRVPLGEPTTPF
                     KRLRVAESARPPIDPALRTSATSKCVAAMLGPNDAWLDPHAINMFFESEWTVQARSNR
                     TGLRLTGPEFTFSERAYSKRPEHGQEPSNILDHGYPVGGISVAGQTAIVLIHDAPSAG
                     GFIIPFTVPRCDLWRLGQARPNEILHFRRVTMGEANAERRRMDALCTATALIETTTQA
                     "
     gene            complement(2016..2876)
                     /locus_tag="VHV99_11840"
     CDS             complement(2016..2876)
                     /locus_tag="VHV99_11840"
                     /inference="COORDINATES: protein motif:HMM:NF014712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyltransferase domain-containing protein"
                     /protein_id="HEX3636162.1"
                     /translation="MLYPEPRFVPGGDCFIEVELGDEMSFDLNVRVHALAAAIRAAQI
                     AGIVELIPELASLLVSYDPDRISYADTIREIQALSDQLSGGAMQEMTSRVFHVPLLYY
                     DPWTAECVDEYRKTYPDKTPDPELMCEHNGLADRATLQRVHSASEYWVAALGFWPGLC
                     SLMPLDPRSRLNAPKYNPPRGWTPKGTIGLGGGLTCIYPDRTPGGYQIFARTPMPTWD
                     RHQRLSAFRESPALFRPGDRVRFVPIQRDEYDFIEEEVNEGRYEHPVIEYQTFSVPKY
                     QAWLQTLGKE"
     gene            complement(2911..3765)
                     /locus_tag="VHV99_11845"
     CDS             complement(2911..3765)
                     /locus_tag="VHV99_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636163.1"
                     /translation="MSHPVLQLSVREPAAQPKGGEWSYWRGWPACAKWITFGVLGLIY
                     FPLLWLALMSVSARPLSGIPFPLSGANYVSLFTNDSWLAPFGISVMIAVGVAVVTAVT
                     ATLVGRALPHSPRAGTLVLLAVLPLFIPGMSMGAAQFIFLRPMLGLTLGFWSIFLGHV
                     VWAFPFSLLIVLVLTTRFDSRLVEAAADLGASGWRTFWDIEFPILRPGIVGAGTFGFL
                     ISFNEVQRSIFLRGTATTMPIWNWEMASSQQSQVPVIFSLETIVLAVVLPLLAALFWV
                     LFVRMDKN"
     gene            complement(3762..4676)
                     /locus_tag="VHV99_11850"
     CDS             complement(3762..4676)
                     /locus_tag="VHV99_11850"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3636164.1"
                     /translation="MSDASIGIPRRGTMRTPLERSPWLLPLFLSVWLTLVIAPLLVLF
                     AYSFFESRNFAMIYKPSLATWESLFTSGRFEVTVRTLRIALTVTVIELALAFPSALWL
                     AKGGCSKTTKALVLALLTIPFFLDLSSRIIVWRGILDENGLINYVLMAAHVIASPIRG
                     MLYTEQAVHFGMIVSYFPMMVLPIYMAISVIDDTLIAAAADLGASPWRILADIVLPLS
                     MPGILAGVVFTLGPALASWIEPNMLGGGFVNMLSNSVDSAYSALRYPVVAALSSFVIL
                     MVGLMLAVLMFLTRRFADLSTSFRNLQS"
     gene            complement(4714..>5088)
                     /locus_tag="VHV99_11855"
     CDS             complement(4714..>5088)
                     /locus_tag="VHV99_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636165.1"
                     /translation="NLKLGDGKTRYAYTTEGYYKWGHGAYIASQAKSRGNMDNIYKML
                     NYFLDGEYRALQARDRGYAGPNMDLAVTYATANGWKADDIANLKATQQKVDRKFTKPF
                     VSTTTPSNSDAIEEEWQRFLNA"
BASE COUNT         1063 a         1612 c         1510 g          903 t
ORIGIN      
        1 ccatcagttt ctgcctgcgc ctttgcagtg agcattcgcg gtcgtagaga tggatcgcgc
       61 gtacgccatc acccatgatc tgcacttcca catgatgtgc acgcccaaca aagcgctcca
      121 gataaaggcc gccgtagccg aaggcgcttt cggcttcgcg ggtggctttg tcgaattcgg
      181 cggcgagttc gtccgcgtct tgcgcgacac gaatgccacg tccaccgccg cccgcggcag
      241 ctttcaacat gaccggatag ccgacccgaa gcgcctcacg ctgcgcctct tcaacatgtt
      301 cgactacgcc atcgctgccg ggtacggtcg gaacgccggc ccgccgcgca caggcgcgcg
      361 cggcagcctt gtcacccatc aatgaaatgg tctcgggcga aggtcccacg aagatcagcc
      421 ctgcgtcctt gagcacctgc gcaaaagccg cattctccga taaaaacccg taccccggat
      481 gcacagcgtc agcgccgctg cgacgggccg cctcaacgac cgcacgcgtg tcgagatagc
      541 tcttcgcagc gggcgccgga cccagcacct cgatgctgtc cgccatgcgt gcagcaagac
      601 tgtcggtatc ggcctcgctg caacccagca cggtttgcaa cccaagctcc cgcgccgcgc
      661 gaatgatgcg caccgcaatc tcaccccggt tagcgacaaa caccttcctg atcgtacgcg
      721 tcatttcaaa cacttcccgt atcgatcacg acgagcacag cgtcagcgtc gacggcatcg
      781 ccatcggcga ccgcgaaact gcgcaccaca cccgctacac ccgcaagtac ctcgttgaac
      841 tgcttcatca actccacaat cccgataatc gtttcggccg agacgctttg cccttcctca
      901 acgagcggcg gcttgccggg tgcggagctg cgataaaacg tccccgggat ttcgcttttg
      961 acttcgaagt cggccatctg gttgtcgccc ttggtttgcg ttacacgtca tgcttgagta
     1021 gtcgtttcga tcagcgctgt cgccgtgcac aacgcgtcca tacgccggcg ctcggcgtta
     1081 gcctcgccca tcgttacgcg gcgaaagtgc aggatttcgt tggggcgtgc ttgtcccaac
     1141 cgccatagat cgcagcgcgg aaccgtgaac ggaatgatga aaccgccggc gctcggcgca
     1201 tcatgaatca gcacgatggc cgtctgcccc gccacgctga tgccgccgac cggatagccg
     1261 tgatcaagaa tgttcgatgg ttcctgtccg tgttccggcc gctttgaata ggcgcgctcc
     1321 gagaaggtga actcgggtcc ggtcagcctc agccccgtgc ggttgcttcg cgcctgaacc
     1381 gtccattcgc tttcgaagaa catgttgatg gcgtgcggat cgagccatgc atcgttgggg
     1441 ccgagcatgg cagcaacgca cttcgaagtt gcgcttgtgc gaagggccgg gtctatcggc
     1501 gggcgggcgc tctctgccac acgaagccgc ttgaatggcg tcgtcggctc gccgagcggt
     1561 acgcgatcgt ctttcttcaa cgcgccattc cccgtcccgc cgatggcggc catatggaac
     1621 gtcgcgcgtg aacccagtac cggctcggtc gttatgccgc ccgataccgc gatataccct
     1681 cgagctcccg tgcgcgccgc gccgcacgtc agcacctgcc ctgccctcac attgagcgtt
     1741 tcccacatcg gcacgggggt gtcatcgagc ttcgcggcca tgtcggcgcc ggtcagcgca
     1801 atcgcgccgt cgcgatgaaa gcgcaccgtc ggcccgacga actgccattc gagcgcggcg
     1861 gtatcgcggt cgttgccgac caacagatta gcaagacgaa acgaccacgc gtctgccggc
     1921 ccggatggcg gaaagccctg ctcccaatga ccaatgcggc caggcaattc ctgcaccgtg
     1981 gtctcgagac ccggtttgac gatatcgagc atccgtcact ccttcccgag ggtttgcagc
     2041 caggcctgat acttcggcac cgagaacgtc tggtattcga tcaccggatg ctcgtagcgc
     2101 ccctcgttca cttcttcttc gatgaagtcg tactcgtcgc gctggatcgg tacgaaacgt
     2161 acgcggtcac ccggccgaaa caaggccggg ctttcgcgga atgcgctcag ccgctgatgc
     2221 cggtcccacg tcggcatggg cgtgcgggcg aatatctgat agccgccggg cgtgcggtcc
     2281 ggataaatgc acgtgaggcc gccgccaagc ccgatcgtgc ccttgggcgt ccagcctcgc
     2341 ggcgggttgt atttcggcgc attgagccgc gagcggggat cgagcggcat cagtgagcag
     2401 agtcccggcc agaagccgag tgctgcaacc cagtattcgc tggcggagtg gacccgctgc
     2461 agtgttgccc ggtcggccag gccgttgtgc tcgcacatca gttcggggtc aggtgttttg
     2521 tcgggataag tcttgcgata ttcatcgacg cactcggccg tccacgggtc gtagtaaagc
     2581 agcggcacgt gaaagacccg gctggtcatt tcctgcatcg cgccacccga aagctgatcc
     2641 gataacgcct ggatttcacg gatggtgtcg gcgtacgaga tgcggtccgg atcataggac
     2701 acgagcaacg acgccagttc gggaatcaac tcgacaatgc ccgcaatctg cgccgcgcga
     2761 atggcggcgg caagcgcatg aacgcgcaca ttcaggtcga aactcatttc gtcgccaagt
     2821 tcgacttcaa tgaagcaatc gccgcccggc acgaagcgcg gctcaggata aagcatcgca
     2881 aggcccttga gagttaagga gagacgtcga tcaattcttg tccattcgca cgaacaagac
     2941 ccagaaaagc gcagccagaa gcggcaggac gactgcaagg acgatcgtct ccaacgagaa
     3001 gatcacaggc acctgcgact gctgcgacga agccatttcc cagttccaga tcggcatggt
     3061 cgtcgccgtg ccgcgcagga aaatggaccg ctgcacttcg ttgaacgaga tgagaaaacc
     3121 gaacgtgccg gcgcctacta tgccggggcg cagtattgga aattcgatgt cccagaaggt
     3181 gcgccagccc gacgcgccca gatcggctgc ggcctcgacg aggcggctat cgaaacgcgt
     3241 ggtcagcacc agcacgatca gcagcgaaaa cggaaacgcc cagaccacgt gaccgaggaa
     3301 gatggaccag aaccccagcg tgagaccgag catgggacgc aggaagatga actgggcagc
     3361 gcccatcgac atgccgggta tgaagagcgg caacaccgcg agcaacacca gcgtgccggc
     3421 gcgcggcgaa tgcggcaacg cccggccaac cagcgtggcc gtcaccgccg tcacaacggc
     3481 aacgccgacc gcgatcatca cgctgatgcc gaagggcgcg agccagctgt cgttcgtaaa
     3541 gagcgacacg tagttggcgc ccgacagcgg aaacggaata cccgaaagcg gacgcgcgct
     3601 caccgacatc aacgcaagcc aaagcagcgg aaagtagatc aggcccagca cgccgaacgt
     3661 gatccatttt gcgcacgccg gccagccgcg ccagtagctc cactcgccgc ccttgggctg
     3721 cgcggcaggt tcgcgaacgg atagctggag gacgggatgg ctcaactctg aagattccgg
     3781 aaagaggtgg aaaggtcagc aaagcggcgc gtgaggaaca tcagcacggc aagcatcagg
     3841 ccgaccatca ggatgacaaa gctcgacaac gcggcaacca ccggatagcg caacgcgcta
     3901 taagcgctat ccaccgaatt gctcagcatg ttgacgaagc caccgcccag catgttcggt
     3961 tcgatccacg atgcaagggc cggtccgagc gtgaagacca cgccggccag aatgccgggc
     4021 atggaaagcg gcaggacgat atcggcgaga atacgccacg gtgacgctcc aagatcggcg
     4081 gccgccgcga tcagcgtgtc gtctatcacg cttatcgcca tgtagattgg caggaccatc
     4141 atggggaagt acgaaacgat catgccgaaa tgcaccgcct gctccgtgta gagcatcccg
     4201 cgtatgggcg acgcaatgac gtgcgccgcc atcagcacat aattgatgag accgttttca
     4261 tcgaggatgc cgcgccacac tatgatgcgc gacgataaat cgaggaagaa cggaatcgtc
     4321 agcaacgcaa gcaccagcgc cttcgttgtc ttgctgcagc cgcccttcgc gagccataac
     4381 gcgcttggaa aagcgagcgc cagttcgatt accgtcaccg tcaacgcaat gcgcagcgtg
     4441 cgcacggtga cctcaaagcg ccctgacgtg aagagacttt cccacgtcgc gagcgacggc
     4501 ttgtagatca tcgcgaagtt gcggctctcg aagaagctgt aggcgaacag caccagcaac
     4561 ggtgcgatca cgagcgtcag ccataccgac agaaacagtg gcaacagcca gggagaacgc
     4621 tcgagcggcg ttcgcatggt gccgcgccgt gggatgccaa tgcttgcatc gctcatggtc
     4681 agatccgaaa gagtaagagg ctcatgcaat gcactaagcg ttcagaaagc gctgccattc
     4741 ttcttcgata gcatccgaat tcgacggcgt cgtggtgctc acaaagggct tggtgaactt
     4801 gcgatcgacc ttctgctgcg tcgccttcag gttcgcgatg tcgtcggcct tccatccgtt
     4861 tgcggttgca tacgtcacgg cgagatccat gttcgggccg gcataaccgc gatccctcgc
     4921 ctgcagcgcg cggtactcgc catcgaggaa gtaattcagc atcttgtaga tgttgtccat
     4981 gtttccacgg ctctttgcct gcgatgcgat ataggcgccg tggccccact tgtaatagcc
     5041 ttcggtggtg taggcgtagc gggtttttcc atcgccgagc ttgaggtt
//
LOCUS       DATASH010000427         4076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3233161,
            whole genome shotgun sequence.
ACCESSION   DATASH010000427 DATASH010000000
VERSION     DATASH010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3233161"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VHV99_11860"
     CDS             complement(<1..232)
                     /locus_tag="VHV99_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636166.1"
                     /translation="MEIIKKYESFHDWYLQGVAADLDAGEVELRLMFDNRKDHARVIF
                     KGASRCLMNDFLIQNIIYSMDVLKDFESDEYKR"
     gene            complement(297..764)
                     /locus_tag="VHV99_11865"
     CDS             complement(297..764)
                     /locus_tag="VHV99_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014252734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm50 family immunity protein"
                     /protein_id="HEX3636167.1"
                     /translation="MSIDNKTWGWELARNSGLLIKIFGDYPSFHDSAVTTFSLQRKRR
                     SFDGVDSKPLPAGRVRYLLDLQLEVLHNRYAAPRSDGDPDYLVVLDIQDIRSSEIDVN
                     AMLEEASIMEISLSDTPDDLIRFDLEPNIGLDIRLTCKEVVVSAIRPYSRSEF"
     gene            complement(1020..2582)
                     /locus_tag="VHV99_11870"
     CDS             complement(1020..2582)
                     /locus_tag="VHV99_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OprD family outer membrane porin"
                     /protein_id="HEX3636168.1"
                     /translation="MKDCRTKNRSGKTSAATFFYCAFTLPALTLGASAYADDIVLEPT
                     ADTRKTQTLAQTFSSPTPQSQANPAGKRRADQGGTVRNDQPDTTNAIVNAEANQTVTP
                     PTPPSSQAASKGFVGDSHLNLQLRNYADYFQAPTSIYRHAWVQGAQVNYESGFTQGPV
                     GFGVDASLFAALKLDGGNGAGNMVHVGKDAGGSQQLAWAYPGMYDIKGRISETVVKYG
                     LQTVTNPFLEPHDNRALPPTFLGVSMVSNDLVHTTLEAGSFTKVDARGHTNLTNLTTS
                     YGGTRIDRLTYVGGTWHYAKNGEMALYVDQADDVWRQYYGSVAQSFGDPTTIKWSGLA
                     NIYSTHDTGASRQGHINSNAYSLSVSAQHGPHALLLGYQQVLGDQFFDYVNETNGIYL
                     TNSMDVDYNAPHEQSLQLRYTFDGKYAGVPGLKAMFWGEYGWGADASAGAADNASASA
                     PLHDLYWKNGEPVHGHHHEFGFIPSYTLQSGRFKDTKITFIAMWHNASRYYSDGNNME
                     YRLVVNVPVKVF"
     gene            complement(2734..2919)
                     /locus_tag="VHV99_11875"
     CDS             complement(2734..2919)
                     /locus_tag="VHV99_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636169.1"
                     /translation="MSLSLLFVARDLAVVKAISQPARFRSPSRVACIRVNDVPKEAPL
                     MEAPLMDARLEAPVGHR"
     gene            complement(3155..3727)
                     /locus_tag="VHV99_11880"
     CDS             complement(3155..3727)
                     /locus_tag="VHV99_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX3636170.1"
                     /translation="MVTTIQQNNPALELGSKIRSLRQRLKRTLDDTAPAAGISKPFLS
                     QVERGLASPSLTSLAGIAQALGVTVQYFVDTPSEERSVCRGDQLKFFGFADSANLFAR
                     LTNLTGGRQLEAILVRMPPGQKRSEVTTHAGEEFVYVIDGEVSLTLEGKTFVLHAGDS
                     AHYESTVPHSWVNTARKESVVVWVGTPRLL"
BASE COUNT          811 a         1163 c         1202 g          900 t
ORIGIN      
        1 ctcgcttgta ctcgtccgac tcaaaatctt tcagtacgtc catcgagtaa atgatgttct
       61 gtattagaaa atcgttcatg agacaccggg acgctccctt gaaaatcacg cgtgcatgat
      121 ctttcctgtt gtcgaacatc aaacgtagct ctacctcgcc ggcgtccaga tcggcggcaa
      181 caccctgcaa ataccagtcg tggaacgatt catatttttt gattatttcc attgcatacg
      241 tttcttcgcc atcggccggc gctcactctt tggcgtgaga aacacggctg tgaggctcaa
      301 aattcgcttc ggctgtaagg tcgaattgca ctgacgacga cctctttgca agttaagcgg
      361 atatcgagtc cgatattggg ttcaaggtcg aagcggatca gatcatcggg cgtatcggat
      421 aaagaaatct ccataatcga ggcttcctcg agcatggcgt ttacatcgat ctccgaggac
      481 cgaatatcct ggatatccaa cacgactaga tagtcgggat ctccatcgga gcgaggagcc
      541 gcgtagcgat tgtgcagcac ctcaagctgg agatcgagta aataacgcac gcggccggcc
      601 ggcagcggct tcgagtctac gccatcgaac gagcgtcgct ttctctgcaa agagaacgtc
      661 gtcacggccg agtcgtgaaa agatgggtag tcgccaaaaa tcttaatgag caagccactg
      721 ttcctcgcca gctcccagcc ccacgtctta ttgtcgatgc tcattggctt ttccctcgca
      781 atatgctgct acctgcaatt gatcggtgac gttggaagat gaatgtcatc tctcactccg
      841 gcatcagaat ccggaagtca aagacaacta tttccatcgg tggtagatgc tccgcaaaag
      901 tgaactcagg acgcaaagaa agctcaccac ccggtgcgtg caggtgttcc ccagccacac
      961 ctgcacgaaa gagaacggct tttatggccc tatacacagg ccccgtacgc ccgctcccct
     1021 cagaacacct tcaccggcac attcaccacc aacctgtact ccatattgtt gccgtcggag
     1081 taatagcgcg aggcgttgtg ccacatcgcg atgaacgtga tcttcgtgtc tttgaagcgg
     1141 ccgctttgca gcgtatagct cggaatgaaa ccgaactcgt ggtgatggcc gtgcaccggt
     1201 tcaccattct tccaatagag gtcgtgcagc ggcgccgacg ccgaagcatt atccgcggca
     1261 cccgccgatg catccgcgcc ccagccatac tcgccccaga acatcgcctt gagcccgggc
     1321 acgcccgcat atttaccgtc gaacgtgtag cgcaattgca gcgactgttc atgcggcgcg
     1381 ttatagtcga cgtccatcga gttggtcagg tagatgccgt tggtctcgtt gacgtagtcg
     1441 aagaactgat cgccgagcac ctgctggtag ccgagcagca gcgcatgcgg gccgtgctgc
     1501 gcggagaccg acaggctgta cgcgttactg ttgatatgcc cctggcggga tgcgccggta
     1561 tcgtgcgtcg agtagatatt cgcaagaccg ctccacttga tggtggtcgg gtcgccaaac
     1621 gattgcgcca ccgagccgta gtattgacgc cagacgtcgt cggcctggtc gacatacagc
     1681 gccatttcgc cgttcttcgc gtagtgccac gtgccgccca cgtaagtcag gcggtcgatg
     1741 cgcgtgccgc cgtacgaggt agtcaggttg gtcagattcg tgtggccgcg tgcatcgact
     1801 ttggtgaaac tgccggcctc cagcgtcgta tgaaccaggt cgttgctgac catcgacaca
     1861 ccaaggaaag tcggcggcaa cgcgcggtta tcgtggggct cgaggaacgg gttggttacg
     1921 gtctgcagac cgtacttcac gaccgtttcc gaaatgcgcc ccttgatgtc atacatgccg
     1981 gggtaagccc aagcgagttg ttgcgagccg ccggcgtcct tgccgacatg caccatattg
     2041 ccggcgccgt tgccgccgtc gagtttgagt gcggcgaaca gcgacgcgtc gacgccgaag
     2101 ccgaccgggc cttgcgtgaa ccccgattca tagttgactt gcgcgccttg aacccacgcg
     2161 tggcgataga tcgaggtggg ggcctgaaag tagtcggcgt agttgcgcaa ctgcaggttc
     2221 agatggctat cgccgacaaa acccttgctc gctgcctggc tcgacggcgg ggtgggcggc
     2281 gtgacggtct ggttagcttc cgcgttgacg atggcgttgg tggtgtccgg ttgatcgtta
     2341 cgcaccgtgc cgccctggtc cgccctgcgc ttgccggcgg ggttcgcttg cgactgtggc
     2401 gtaggagacg aaaacgtttg cgcgagtgtt tgagttttgc gcgtatcggc ggtgggctcc
     2461 aatacgatgt catcggcgta ggcactcgcg ccgagagtca gcgcagggag cgtgaatgcg
     2521 cagtaaaaga aggtagctgc actggttttt cctgaccggt tttttgttcg gcaatctttc
     2581 atgtggaacg tctctgttat tttggtgaat cattggctgg cggttagcca gtccacgttt
     2641 agcgttcgtt ctgatgatga caggacgaac gaatccagcg gcgcgacgga ctgccgcgct
     2701 aagaacaggg tttagcccgt taggctcctg gctttagcga tgccccacgg gcgcttccag
     2761 tcgcgcgtcc atcaaggggg cttccatcaa gggggcttcc ttagggacat cattcacgcg
     2821 aatgcacgcg acgcgtgacg ggctgcggaa tctcgctggc tggctgatcg ccttgacgac
     2881 cgcgagatcg cgcgcgacga acagcagcga cagcgacaac tcgcgttgcc gaacgtaagg
     2941 acgagcgcgg tcagcgccgt cgcgtggacg aacggggttt tcatcggttg agatcgcagg
     3001 aaaggtcgag aagaacgcgc cggcgccagg gcgcctggtt atccgcggtg ctcgggccgg
     3061 ggaggccctt gttgtttgct cggcatgcag gattccttaa atttggtcat gcaggcaatc
     3121 cctggccggc tcgaccggaa aaggtgcctg ctttttagag cagtcttggt gtgcccaccc
     3181 agacgaccac cgactccttg cgtgcggtgt tcacccagct gtgcggcacc gtcgattcgt
     3241 aatgcgcgct gtctcccgcg tgcaacacga acgtcttgcc ttccaatgtc aacgacactt
     3301 cgccgtcgat cacataaaca aattcttcgc cggcgtgcgt ggtcacttcg gagcgtttct
     3361 gcccgggcgg catcctcacg aggatcgctt cgagctgacg gccgcccgtc aggttggtca
     3421 gccgcgcaaa cagattggct gagtccgcaa aaccgaaaaa cttcaactga tcgccacggc
     3481 acaccgagcg ttcttcgctt ggcgtgtcga cgaaatactg cacggtcacc ccgagtgctt
     3541 gcgcgatccc cgccaacgac gtgagggacg gtgacgccag gccgcgttcg acttgtgaca
     3601 aaaacggctt ggaaatacct gccgcggggg ccgtgtcgtc cagggtgcgc ttgagccgtt
     3661 gccgaagtga gcggatcttg ctgccgagtt cgagtgccgg attgttctgt tgaattgtcg
     3721 tgaccatagc aggaggaaat tagcccgtca aaatttgttt gatataagta aatatggttt
     3781 gatagcggtt taggccgggg ctgcggccat tcagcgcccg gttatgcgag cgaccgcttt
     3841 tcgagacaac attctatgtt gccagaaccg gaatgcgcgc cggtcgtctt gaccggtgtt
     3901 gatccgcggg ccgttttcca tcaatcagtt tttcgcggcg cgtggcccct ccacgcgccg
     3961 ctgcaaccgt aagagcaaga ggcacatgat gaattcatcc aagcccgcgg gcgcaccggt
     4021 ctcgcagacc cgttcgttct cgacggtctt cctgatcgag atgtgggagc gctttg
//
LOCUS       DATASH010000428         1769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5208505,
            whole genome shotgun sequence.
ACCESSION   DATASH010000428 DATASH010000000
VERSION     DATASH010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5208505"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(377..>1769)
                     /locus_tag="VHV99_11885"
     CDS             complement(377..>1769)
                     /locus_tag="VHV99_11885"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX3636171.1"
                     /translation="IAMLTGADAPAIDAVVLDLVMPGLDGMGVLAKIRDAGLSVPVIV
                     QTAHGGIDNVISAMRAGAQDFVVKPIGMERLQVSLRNALNTSALKGELQRIRHSREGR
                     LTFSDIITRSDAMASVLRTAQKAAGSQIPVLIEGESGVGKELFARAIHGSGERKAKPF
                     VAVNCGAIPDNLVESILFGHEKGAFTGATERHTGKFVEASGGTLFLDEVSELPLTAQV
                     KLLRALQEGAVEAVGGRKPVKVDVRIISATNRKLLDRVKSGHFREDLFYRLHVLPLTV
                     PPLRARREDIPHLLRHFLARFGAEENRSITGISGEAMAHLSGLDWPGNIRQLENAVYR
                     AVVMSDGDQLGPADFPSMPSHATVAPQAPSEPLVVSPAILHSAPAMVSGSEIPIAPLP
                     AHGTLAMLTSAGEMRPLEDMENEIIRFAIAHYRGQMSEVARRLKIGRSTLYRKLDEAN
                     GQEPSPDDTKETN"
BASE COUNT          309 a          564 c          565 g          331 t
ORIGIN      
        1 gtcaaatccg ccacggttgt tcgaagagtc tcgcatcgtc acaccccttt gggtgaaccg
       61 ccccggcaag gaacagttcc ggtttccgtc ggtcggtatc ctcaagccag cccgtctggt
      121 ctgtacggct gcacgcgaca agtcactacg cacaccacag acgatacaga caacacccct
      181 ttcccagcag accacgctag acggggcaac tcacgacaaa ctgaccacaa ctcctttgca
      241 acggatgatg cgcttttgcc acgcacaccc ccctttgtct gtcaccggca agccacggtt
      301 cgaaagttct gcgatgaccg aaggttgtgt cgattttcgg ttccgatgcc cagccgcaac
      361 agtcaactgt ttcgcctcaa ttagtctctt tcgtgtcgtc gggcgaaggt tcttgtccgt
      421 tggcctcgtc gagcttgcga tagagcgtgg agcggccgat tttcaggcgc cgcgccacct
      481 cggacatctg gccgcggtag tgtgcgatag cgaaacggat gatctcgttc tccatgtctt
      541 ccagcggccg catctcgccc gcgctggtca gcatcgccag cgtgccatgg gcgggtaagg
      601 gcgcaatagg tatttcacta ccggaaacca ttgcaggcgc gctgtgcagg atcgccggcg
      661 agaccaccaa cggctcgctg ggcgcttggg gggcgacggt tgcgtgagag ggcatggagg
      721 ggaagtcagc aggccccagc tggtcgccgt cgctcatgac caccgcgcga tacaccgcgt
      781 tctcgagctg gcggatgttg ccgggccagt cgagccccga gagatgtgcc atcgcctcgc
      841 cgctgatgcc ggtgatgctg cgattttctt ccgcgccgaa gcgcgcgagg aaatgccgca
      901 agagatgcgg gatgtcttcg cgccgcgcgc gcaacggggg gaccgtcagg ggcagcacat
      961 gcaggcgata gaacaggtct tcgcggaagt ggccgctttt gacgcgatcg agcagcttgc
     1021 gattggtcgc ggagatgatg cggacatcga ccttgacggg cttgcgtccg ccgaccgctt
     1081 cgaccgcgcc ttcctgcagc gcgcgcagca gcttcacctg cgcggtcagc ggcagttcgg
     1141 agacctcgtc caggaacagc gtgccgccac tggcttcgac gaacttgccg gtgtggcgtt
     1201 cagtcgcgcc ggtgaaggcg cccttctcat ggccgaacag gatcgattcc acgaggttgt
     1261 cggggatcgc gccgcagttc actgcgacga acggctttgc cttgcgctcg ccgctgccat
     1321 ggatggcgcg agcgaacaat tccttgccga cgccggattc gccctcgatc agcacgggga
     1381 tctgcgagcc tgccgccttc tgcgcggtgc gcagcacaga ggccatcgca tcgctgcggg
     1441 tgatgatgtc agagaatgtc agccggcctt cgcggctgtg gcggatgcgc tgcaattcgc
     1501 ccttcagcgc gctggtgttg agcgcgttgc gcaacgacac ctgcagccgc tccatgccga
     1561 tcggcttgac cacgaaatcc tgggcgccgg cgcgcatcgc ggagatcaca ttgtcgatac
     1621 cgccgtgagc ggtctgcacg atcacgggaa cggaaaggcc ggcgtcgcgg attttggcca
     1681 gcacgcccat gccgtcgagg ccgggcatca cgaggtcgag cacgacggcg tcgatcgcgg
     1741 gcgcatcggc gcccgtcagc atggcgatc
//
LOCUS       DATASH010000429         3455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5606325,
            whole genome shotgun sequence.
ACCESSION   DATASH010000429 DATASH010000000
VERSION     DATASH010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5606325"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VHV99_11890"
     CDS             complement(<1..224)
                     /locus_tag="VHV99_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid hydroxylase"
                     /protein_id="HEX3636172.1"
                     /translation="MFHLIFSSLDSFVSAVQTLLYVDVVQPLLFRFDLMGYDEDTYDS
                     LYWVIVGVFEVLAMYAVLRPLEALRPVEQW"
     gene            complement(256..1275)
                     /locus_tag="VHV99_11895"
     CDS             complement(256..1275)
                     /locus_tag="VHV99_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEX3636173.1"
                     /translation="MRKFFLRGLTATFAAATALIATAGVFSPAAHANNVIVLNSGEAT
                     LSLIDETTRQVVGTVPTGKEPHHLMATPDNSSLIVANSVSNNLMFVDPKSGQVQRWVE
                     NIEDPYQIGFSPDRKWLVTTGLRLDRLDIYHYDGLKNQMTLASRLPLAVMPSHMAFTN
                     DSKTVFVTLQVSGELAAIDLATQTVKWKMKVGKVPAGLWMTPGEKYLLIGMTGADYVA
                     VVDWRNQKIVKTIQTGKGAHNFRSLADGKHVAVSNRMASTISIIDEDTLTNVGDITGL
                     MPGPDDMELSADKRYLWVTFRFAKHVGIIDLNTRKLIQTIAVGRSPHGIYFFNRAPVT
                     APNGA"
     gene            1988..>3455
                     /locus_tag="VHV99_11900"
     CDS             1988..>3455
                     /locus_tag="VHV99_11900"
                     /inference="COORDINATES: protein motif:HMM:NF017476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636174.1"
                     /translation="MAQAMLAGAALMVAASAAHAQSTAATYYFAADPSNPGLTQNAQA
                     TAAGTVAAGGGALASGLSSAAIGDGAKAIDTGAVALGTTALASQKGATAIGFSTVASG
                     ISSTANGSAAWATGDYSTALGGLSNALAQGATALGAYSTAMGTDSVAVGYNATAAADN
                     SVALGANSTTTADLKKAAVVPTGAGATTVEATTASAEVSVGSAGNERRVTNVAAGAAA
                     TDAVNVSQLSAVDGKVNTLAQSETLHYFKANGAGVGSDDAVANGTYAVAAGANASATQ
                     ANSVAVGYNAASTGGSSMALGANSVASTSVATAVGAGAEATGAAATAIGANSSATALG
                     SVALGNMAEASADGSVALGQGSSAAADGSVALGQGASAAAAGSVALGQGSTADRANTV
                     SVGSAGSERQITNVAAGTEATDAVNVSQLAAATASASRYFKATGIAGSGDDASATGDH
                     SVAIGSGADASGAKSFAAGTTAWASATDSTAVGTLATAT"
BASE COUNT          732 a         1063 c         1033 g          627 t
ORIGIN      
        1 tcccactgct ccaccgggcg cagcgcttcg agcggacgca ggaccgcgta cattgccagc
       61 acctcgaaga cgccgacgat cacccaatac aggctgtcgt aggtatcttc gtcgtagccc
      121 atcagatcga agcggaacag cagcggctgc accacgtcga cgtacaacag cgtctgcacc
      181 gccgagacga agctgtcgag agaagaaaaa atcagatgga acatggtgcg ggcccgttac
      241 ttccttgtta cagcatcaag cgccgttcgg cgccgtaacc ggcgcgcgat tgaagaaata
      301 gataccgtgc ggcgaccggc cgacggcaat ggtctggatc agcttacgcg tgttcaggtc
      361 gatgatgccg acgtgctttg cgaagcggaa ggtaacccaa agatagcgct tgtccgcgga
      421 aagttccatg tcgtccggcc ccggcatcag accggtgatg tcgccgacat tggtgagcgt
      481 gtcttcgtcg atgatgctga tcgtgctcgc catacggttc gacaccgcca catgcttgcc
      541 gtcagccagc gagcggaagt tgtgcgcgcc cttgcctgtc tggatcgtct tgacgatttt
      601 ctggttgcgc cagtcgacca ccgcgacgta atcggccccg gtcatgccga tcagcaggta
      661 tttctcgccc ggtgtcatcc acaggccggc cggcaccttg ccgaccttca tcttccactt
      721 gaccgtttgc gtggccagat cgatcgcggc cagctcgccc gacacctgca gcgtgacgaa
      781 aaccgtcttg ctgtcattgg tgaacgccat gtggctcggc atcaccgcga gcggcagacg
      841 cgaggccagc gtcatctggt tcttcaggcc gtcgtaatga tagatgtcga gacggtccag
      901 gcgcagcccg gtggtgacaa gccacttgcg gtcgggcgaa aagccgatct ggtaaggatc
      961 ctcgatgttc tcgacccagc gctgcacctg gcccgacttc ggatcgacga acatcaggtt
     1021 gttggacacc gaattggcga cgatcagcga cgaattgtcc ggcgtggcca tcaggtgatg
     1081 cggttccttg cccgtgggca ccgtgccgac gacctggcgg gtggtctcgt cgatcaggct
     1141 cagcgtggct tccccggaat tgagcacgat cacgttgttg gcgtgggccg ccggagaaaa
     1201 aacgcctgcg gtggcgatca gtgccgttgc tgcggcgaac gtggcagtca agccgcgaag
     1261 aaaaaattta cgcatgaaaa ctcacttcgg agaacaaacg tcattgtaga acgtttgcca
     1321 gggcccgcgg ttgatgtgga taccggttaa agctacgaaa aaacaggggc gcgacagaac
     1381 cggcagcgcg ctctgtcagc ggtttccgtt gagttgcgtg actcgcgtgg gttttgcgta
     1441 agaaagtcgg cagccgattt gacttcaagc caataaattc taacgcgata aattattaca
     1501 ttcttaaatt ttagattttt acataagaca aaacaggatg attctttatt ttcaacaaat
     1561 aaaaaacact tattcaaact taataacaac gaattatttt atttcaacca tttaggaatt
     1621 ggcttattcc gcctctgccc caccaggtct gacaatcccg ccgatcatcg cccatgcact
     1681 taatccagtg gactcaatgg cgtgacgcag gcgattcgga cgcgcgaata attcagcagg
     1741 acacggaaaa cgaacgacgg aattaccgcg atcgacatgt tctatttgtg cgcgacaaat
     1801 ctgggtgatg acgcctttat ccgaatcagc atgctttcac gcgccgtcca cactcgtaat
     1861 tctgcggagt ttcagaggta tgaataagat ttacaaatcg gtttggtgcg aagcgacggg
     1921 aacgtgggta gccacgtcgg aaacggcgcg ggcgaagaca aagcgcggga cggcgcggaa
     1981 actggctgtg gcgcaagcga tgttggccgg cgcggcattg atggttgcag cgtctgcagc
     2041 acatgctcaa agcaccgctg cgacctatta ttttgccgcc gatccgtcga accccggctt
     2101 gacccagaac gcacaagcca cggcagccgg aaccgttgcc gccggcggcg gcgcccttgc
     2161 atcgggactg agctcggcag caatcggcga cggcgcaaaa gccatcgaca ccggagctgt
     2221 cgcattaggt acaaccgcgc tcgcttccca aaaaggtgct accgcaatcg gctttagcac
     2281 ggtcgccagt ggaatcagct ccacggcgaa cgggtcagca gcctgggcaa caggcgatta
     2341 cagcacggcc ctcggcggcc tctccaatgc cttggcgcag ggcgcgaccg cactgggcgc
     2401 gtatagcacc gccatgggaa ccgactcggt agcggtcggc tacaacgcga ccgccgcagc
     2461 cgacaacagc gtcgccctgg gcgcaaattc gactactacc gccgatctca agaaggcagc
     2521 tgtcgtgccg accggcgcag gcgcgaccac cgtcgaagcc accaccgcaa gcgccgaagt
     2581 ctcggttggc tccgccggca atgaacgccg cgtgacgaac gtcgcggccg gcgccgccgc
     2641 gaccgatgcc gtcaacgtca gccagttgag cgccgtcgac ggcaaggtca atactctcgc
     2701 ccaaagcgaa accctgcact acttcaaggc gaatggcgcc ggcgtcggtt ccgacgatgc
     2761 agtcgcaaac ggcacgtacg cagttgcggc cggtgcaaac gccagcgcta cgcaggccaa
     2821 ttccgtcgca gtcggctaca acgccgcctc gactggtgga agctcgatgg cgctcggcgc
     2881 caattccgtt gccagcacgt cggtggccac cgcagtaggt gccggtgcgg aagcgacggg
     2941 cgcggccgcc actgcaattg gcgccaattc atccgcaacg gcattgggca gcgtcgcact
     3001 cggcaatatg gccgaggcca gcgcggacgg ttcggtcgca ttgggtcaag gttcgagcgc
     3061 tgctgcggac ggttccgtcg cattgggtca aggtgcgagc gccgctgcgg ccggttctgt
     3121 cgccctgggc caaggctcca ccgccgatcg cgccaacacc gtttcggttg gctcggcagg
     3181 cagcgaacgc cagatcacca acgtcgcagc cggcacggaa gccaccgacg cggtcaacgt
     3241 cagtcaactg gcggccgcca cggcgagcgc ttcgcgctat ttcaaggcga ccggcatcgc
     3301 cggcagcggt gacgatgcct ccgccaccgg tgaccattca gtcgcaatcg gcagcggcgc
     3361 cgacgcgtca ggcgcaaaat ccttcgccgc cggtacaacc gcatgggcga gcgctacgga
     3421 cagcaccgcc gtcggcacgc tcgccaccgc caccg
//
LOCUS       DATASH010000430         7299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_659234,
            whole genome shotgun sequence.
ACCESSION   DATASH010000430 DATASH010000000
VERSION     DATASH010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7299
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659234"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1530)
                     /locus_tag="VHV99_11905"
     CDS             complement(<1..1530)
                     /locus_tag="VHV99_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014898342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX3636175.1"
                     /translation="MQSTSPTSPPPSPQKRKRPLWIKLIIGFVGLIVAGVLCVLLVLG
                     YALVVATPNLPSLDALTDYRPKVPLRIYTADHVLIGEFGEERRDIVHIQDVPDSLKKA
                     VLAIEDARFYDHGGVDLTGIARAGFVALTNGHATQGASTITMQVARNFFLSSEKTYTR
                     KIYEMLLAYKIESKLTKDQILEVYMNQIYLGQRAYGFASAARVYFGKDLKDLTLAESA
                     MLAGLPKAPSAYNPVVNPKRAKIRQEYILQRMHELHYITQEQYDEASKQPLVVKGAGK
                     EFSVHAEYVAEMVRQMIYAQYREEAYTRGLNVVTTIDAADQDVAYRALRKGLMDYERR
                     HGYRGPEAFIDLPSDADDREQAIDDALLEHPDNGEIIAAVVTGASPKQVQATFIDGNV
                     ATIQGDGLRFAQFALGARAQPNQRVRPGAIIRLVKNDDGNWSITQLPQVEGAFISVVP
                     QDGAIRALVGGFDFNKNKFNHVTQAWRQPGSSFKPFIYSASLEKGLGPATVINDAPLF
                     FS"
     gene            1784..2776
                     /gene="pilM"
                     /locus_tag="VHV99_11910"
     CDS             1784..2776
                     /gene="pilM"
                     /locus_tag="VHV99_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEX3636176.1"
                     /translation="MTFKNSWLQGVQLGAQRFAAGIDVGSQAVRLVVISQHARAPAAL
                     HIEYVSTVPLGAGAMAGTEIADRHAVARALRDAFAGLPHACAAHALRCAMALPAAATL
                     TTTVPLTRLAALSGCAEDGGHELAELEPAVMSEVERIAGLERHALAVDWFVDETRSPI
                     RSVTIAATARQHLEARIECAATAGISLSAIDGEPHAALRAMRYAASYELDPHEPYVAL
                     WVGTDGVYGWRIVDDCLVGEMRYPAPEHADLADALRDLVHGPVLDCALLSGEVDLLDG
                     VGFSIADIADVLGCTVLPFECAALGGLVRELHDPLLHEPTAAVAFGLALRGVLE"
     gene            2827..3498
                     /locus_tag="VHV99_11915"
     CDS             2827..3498
                     /locus_tag="VHV99_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial assembly protein"
                     /protein_id="HEX3636177.1"
                     /translation="MRGARGVSLRLGGFNLLPYRQRNAQLARRRRLLEFVAAALAGFA
                     AVLALVGWHALETARLDAQRASIEQSLTQLAMPLAEHARLLRAQDEQRKGAARATKLS
                     ESLTHLRDLLDALSFEPGDGVVLQQLRQREHETELLATSRGPIASAEWLKRLSAIRGV
                     KGAEVSDLHRSTPRGGALAVASATGPIEFGAHLRWDDPPRKTALLPRPAAQRPMKSEQ
                     SGGSK"
     gene            3495..4418
                     /locus_tag="VHV99_11920"
     CDS             3495..4418
                     /locus_tag="VHV99_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636178.1"
                     /translation="MSTTFVEFGGAANARLSFSQWVKQVRLPLVAWSRRRRWAVALLI
                     AALVFGLGAHGWVLADLGGLEASRTALHAATRHLADARRALTQLPALRREVAAKPGGP
                     LSAPWTSADDVRIVSELAAQNSVSLLSVDPGAATGSGAESMRPIQLTAHTDFVHLMAF
                     LRGLSDLPVLIVPVDVTVNREAASLAVRATLQVFSALRPVPSTPTADAFADESLDQDD
                     EEDIVFFDPFSIPQMLASGEQQPGASQLRLVGLLHDRTRGLALLDTPDGTTTVVSGQQ
                     LGTERVTRLDALSITLANGGAMRTLALTEAS"
     gene            4763..6430
                     /gene="pilQ"
                     /locus_tag="VHV99_11925"
     CDS             4763..6430
                     /gene="pilQ"
                     /locus_tag="VHV99_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HEX3636179.1"
                     /translation="MVISAGIAHASTPPLPPLPAYLPLDEAIALPDARYDVPPLPRGS
                     AAEVPNPFRDDSAVEPDADPGAVSGISRAAATSVAGRADYLADDLANDADGSVPLARR
                     STTSARHDVAAAAARDELTGATALEGPPVPLPPLARLSGAPKTPADAKLAADDKPISL
                     NFQRAELGAVLNAFAQFTGLNIVASERVRGAVSLRLDKVPWRTAFDTLLDVNGLAMER
                     HGNVIWVAPSADLAARERLRFEAHARAADLEPLASRTFELHYARAEDVRRLLTGAGNQ
                     RVLSKRGAATADPRTNLLFVTDLEARLAQITALLASVDRPTRQVLIEARIVEGEHGFS
                     RNLGVRLSMAAASADGTARGLMGGKDGTVYDLSARPISGFDAATAGLTLFAAGATRLL
                     NIELSALEAEGRGEIVSSPRVVTADRMKAVVEQGTELPYQAKVGQGVSGVQFRRATLK
                     LEVEPQIMPDGRVVLDLDVAKDSVGEQTDAGPAINTKHVQTRVEVEDGGTVSIGGIYA
                     TDDRDDVTRVPVLGKIPVLGALFRHRAHRDQRSELAVFITPRVVQKN"
     gene            6537..7088
                     /locus_tag="VHV99_11930"
     CDS             6537..7088
                     /locus_tag="VHV99_11930"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012402163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEX3636180.1"
                     /translation="MQARDAHANVFFVGLMGAGKTTVGRAIARRLDRPFFDSDHEIEA
                     RTGARIPVIFELEGEAGFRDREAHVISDLTGRENIVLATGGGAVLRPENREALQNRGV
                     VIYLRANPHDLWLRTRRDKNRPLLQTEDPKARLEALYEVRDPLYRECAHFVIETGRPS
                     VNGLVNMVLMQLEMAGVAKHPAS"
     gene            7097..>7299
                     /locus_tag="VHV99_11935"
     CDS             7097..>7299
                     /locus_tag="VHV99_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004545609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEX3636181.1"
                     /translation="MITVNVELGERAYPIHIGADLIGQTALFAPHIAGSSVTIVTNTT
                     VEPLYGDQLRAALAPLGKQVSTVV"
BASE COUNT         1226 a         2316 c         2374 g         1383 t
ORIGIN      
        1 gctgaaaaag agcggcgcgt cattgatcac cgtggccggt ccgagtccct tttcaagcga
       61 ggctgagtag atgaacggct tgaagctcga acccggctga cgccacgcct gggtcacgtg
      121 gttgaacttg ttcttgttga agtcgaatcc gccgaccagc gcgcgaatcg cgccatcttg
      181 cggcaccacc gaaatgaacg cgccttccac ttgcggcaac tgcgtgatcg accagttgcc
      241 gtcgtcattc ttcacgaggc gaatgatcgc accgggccgc acgcgttgat tcggctgcgc
      301 acgcgcgcct agcgcgaact gcgcgaagcg caggccgtcg ccctgaatgg tcgcgacgtt
      361 gccgtcgatg aaagtggcct gcacctgctt cgggctcgcc cccgtcacca cggcggcaat
      421 gatttcgccg ttgtcagggt gctcgagcaa cgcgtcgtcg atggcctgtt cgcggtcgtc
      481 cgcatcggat ggcaaatcga tgaacgcttc ggggccgcga tagccgtggc gccgttcgta
      541 gtccatcaga cctttgcgca gcgcccggta agcgacgtcc tgatcggccg cgtcgatggt
      601 ggtcacgacg ttcaggccgc gcgtgtacgc ctcttcgcga tactgtgcgt atatcatctg
      661 ccgcaccatt tccgcgacgt actccgcgtg cacgctgaac tccttgcccg cgcccttgac
      721 gacgagtggc tgcttgctcg cctcgtcgta ttgttcctga gtgatgtagt gcagttcgtg
      781 catgcgctgc agaatgtact cctgacgaat ctttgcgcgt ttcggattga ccaccgggtt
      841 ataggcggac ggcgctttcg gcaggcccgc cagcatggcc gattccgcca gcgtcagatc
      901 cttcagatct ttaccgaagt acacccgcgc cgcactcgca aagccatacg cgcgctgacc
      961 gagatagatc tgattcatat acacctcgag aatctgatct ttcgtcagtt tcgactcgat
     1021 cttgtacgcg agcagcatct cgtagatctt gcgcgtgtag gtcttctcac tcgaaaggaa
     1081 gaagttgcgc gccacctgca tggtgatcgt gctggcgcct tgcgtggcgt gaccattggt
     1141 cagtgcgacg aaaccggctc tcgcgatgcc cgtgaggtcg acgccgccgt gatcgtagaa
     1201 gcgcgcgtct tcgattgcca gcacggcttt cttcaggcta tccggcacgt cctggatatg
     1261 gacgatgtcg cgccgctctt ccccgaattc gccgatcagc acgtggtcgg ccgtatagat
     1321 gcgcaatggg accttcggcc ggtagtcggt cagcgcgtcg agcgacggca ggttcggcgt
     1381 ggccaccacc agcgcatagc cgagcaccag cagcacacac aggacgcccg cgacgatcag
     1441 acccacaaag ccgatgatga gcttgatcca caacgggcgt ttgcgctttt gcggcgaggg
     1501 cggcggggac gtaggagacg tggattgcat atggacacca aaaagcagtc ccgcgattat
     1561 agccgccccg cttttcagct ttcgtaatgc ttaccgacag tttttgacaa ggctgcgcgc
     1621 catgcgggaa tgacttcact gtagacgctt tgatggcgtg ccgggcgcgc gtcgcgcaac
     1681 gttagccatt tggctgagtg tcgcgcggcg gcgcggctgc gcacaattct ccctcgtaac
     1741 tcgcgttcga atggttcgtc gcgtgagtct gagggagggc gtgatgacgt ttaaaaattc
     1801 gtggcttcaa ggtgtgcaac tcggtgcgca gcgtttcgct gcgggaatcg atgtgggatc
     1861 gcaggcggtg cgcctcgtcg tgataagtca gcatgcgcgc gcacctgccg cgctgcatat
     1921 cgaatacgtg agcacggtgc cgctcggcgc cggcgcgatg gccggcacgg agatcgccga
     1981 ccggcatgcg gtggcgcgtg cattgcgcga cgcattcgcc ggtctgccgc atgcgtgtgc
     2041 cgcgcatgca ttgcgttgcg cgatggcgtt gcccgccgcg gcaacgttga ccaccaccgt
     2101 gccgctcaca cggctcgccg cgctaagcgg ttgtgcggaa gacggcggcc atgaactcgc
     2161 cgaactcgag ccggcggtga tgagcgaagt cgagcgtatc gcagggctcg agcggcatgc
     2221 attggctgtc gactggttcg tcgacgaaac gcgttcgccg attcgttcgg tgacgatcgc
     2281 cgcaaccgcc cgccagcatc tcgaagcgcg tatcgaatgc gcggcgacgg ccggcatttc
     2341 gctcagtgcg atcgatggcg aaccgcatgc cgctttgcgc gcaatgcgct atgcagccag
     2401 ctacgaactc gatccgcacg aaccgtatgt cgcgctctgg gtgggcacgg acggcgtgta
     2461 cggctggcgc atcgtcgacg actgcctggt cggcgaaatg cgctacccgg cgcctgaaca
     2521 tgctgatctg gccgacgcgt tgcgtgatct cgtccacggt cctgtactcg actgtgcgtt
     2581 gttgagcggc gaggtcgatc tgctcgacgg cgtgggtttc tcgatcgcgg atatcgccga
     2641 cgtgttgggc tgcacggtgc tgccgttcga atgtgcggcg ctgggcggcc tcgtacgtga
     2701 attgcacgat ccgttattgc atgagcccac cgctgcggtc gcatttgggc tggcgttgcg
     2761 cggggtgctc gaatgatgcg cgcgttctcc ggtctggatg cggcggccct ggccacgcgg
     2821 acttctatgc gtggcgcgcg cggcgtatcg ctgcggctcg gcggcttcaa tctgctgccg
     2881 tatcggcaac gcaacgcgca actcgcgcgc cggcggcgtc tgctcgaatt tgttgccgcg
     2941 gcgctcgctg gttttgcggc cgtgctcgcg ttagttgggt ggcacgcatt ggaaacagcg
     3001 cggctcgatg cacaacgcgc gtcgatcgaa caatcgctca cgcaactggc catgcccctc
     3061 gctgagcacg ccaggttgct gagagcgcaa gacgaacaac gcaaaggcgc ggcacgtgca
     3121 acgaagttgt ccgagtcgct cacgcatttg cgcgatctgc tggatgcatt gagtttcgaa
     3181 cccggtgacg gtgtggtgct acagcagttg cgtcagcgcg agcatgagac ggagctgctc
     3241 gcgacctcgc gcggacctat cgcgtcggcc gagtggctta aacgcttgag cgcgatccgc
     3301 ggcgtgaagg gcgcggaggt aagcgacctg catcgctcga caccacgcgg cggtgcgctt
     3361 gcggtggcaa gcgcgactgg cccgatcgaa ttcggcgcgc atttgcgctg ggacgaccca
     3421 ccgcggaaaa cggcgcttct gccacgcccc gctgcgcaac gtcccatgaa atctgaacaa
     3481 tcaggaggtt cgaaatgagt acgacttttg ttgagttcgg cggcgcggcg aacgcgcggc
     3541 tttctttttc tcaatgggtg aagcaggtgc gcttgccact cgttgcctgg agtcgtcggc
     3601 gccgttgggc ggtcgccttg ctgatcgctg cgctggtgtt cggacttggt gcgcatggct
     3661 gggttctggc cgatctcggc gggctcgagg cgagccggac ggcattgcat gcagccacgc
     3721 ggcatctcgc ggatgcgcgc cgcgcgttga cgcagttgcc cgcgttacgc cgggaagttg
     3781 ccgccaagcc cggtggaccg ttgtctgcgc cgtggacctc ggcggacgat gtgcgcatcg
     3841 tgtccgaact ggcggcgcag aatagtgtct ccttgctctc ggtagacccg ggcgccgcca
     3901 ccggttcagg tgcagaaagc atgcgtccga tacaactcac cgcgcacacc gatttcgtcc
     3961 acctgatggc atttctgcgc gggctgtcgg acctgccggt gctgatcgtt cccgtcgacg
     4021 tgacggtcaa ccgcgaggcc gcttcgttag cggtgagggc gacgctgcag gtattcagcg
     4081 cgttgaggcc ggtgccttca acccctactg cggacgcgtt tgccgacgag agcctcgatc
     4141 aggatgacga agaagatatc gtcttcttcg acccattctc gattccgcag atgctcgcgt
     4201 ccggcgagca gcagcctggc gcttcgcaac ttcggctggt cggcctgctg cacgatcgaa
     4261 cccgcgggct ggccttgctg gatacgccgg atggcacgac cacggtcgtg tccgggcagc
     4321 aactcggtac tgagcgcgtt acgcggctcg acgcgctcag catcacgctc gccaatggcg
     4381 gcgcgatgcg cacgctggca ctgacggagg cgtcctgatg aggctaatga agccgttcgg
     4441 tgccgcggcg gcgtgcgccg gcgttcgtgt gtggtctggc tttggcgtgg gtcgcgggat
     4501 gccgggaaac atgcgcggga gcgcggtttc caacttcggt cgtagctccg ttcgcagggc
     4561 ggttcacaat ccggttcgca gcctggctag gagtgcggtt cgcagtccgg ttcgcagcct
     4621 ggcacacggt gcggttcgca atccgattta cagcccgcct cgcagtttgg cccagagtcc
     4681 ggttcgtagc gcggttcaca ggtcggttca ccacccgctt cagagcccgc ttcgcaaccc
     4741 gcttcgcagc ctggtcgcgg gtatggtgat aagcgccggc atcgcgcacg cttcgacccc
     4801 accgcttccg ccgctacccg catacctgcc gctcgacgaa gccatcgcgc tgcccgatgc
     4861 tcgatacgac gtaccgccgc tgccgcgcgg cagcgcggcc gaggtcccga acccgttccg
     4921 cgacgattcc gctgttgagc ccgacgccga ccccggcgct gtgtctggca tatcgcgggc
     4981 ggcggcaacc tccgtggctg gccgcgccga ctacctggcc gacgacctgg ccaacgacgc
     5041 agacggctca gtgccgctag cgaggcgctc cacaacctcg gcccgccacg acgtcgcagc
     5101 agcagcggcc cgcgatgagc tcactggcgc cacggcgctc gaaggaccgc cggtgccgct
     5161 gcctccgctc gcccgtctga gcggtgcacc gaaaacgcct gcagacgcca aactcgccgc
     5221 cgacgacaaa ccgatctcgc tcaacttcca gcgcgccgaa ctcggtgcgg tgctcaacgc
     5281 gttcgcccag ttcaccggac tgaacatcgt tgcgagcgag agagtgcgcg gcgccgtttc
     5341 attgcgcctc gacaaggtgc cgtggcgcac cgcgttcgac accctgctcg acgtcaacgg
     5401 actcgcaatg gagcgccacg gcaatgtgat ctgggtcgcg ccgagcgcgg atctggccgc
     5461 gcgcgaacga ctgcgtttcg aagcccacgc cagagccgcc gatcttgagc cgctcgcaag
     5521 ccgcacgttc gagctgcact acgcgcgcgc cgaggacgtg cggcgtctgc tgacgggcgc
     5581 cggcaaccag cgcgtgctgt ccaaacgcgg tgctgcgacg gccgatccgc gcaccaatct
     5641 gctgttcgtc accgatctcg aagcacggct cgcacaaatc acagccttgc ttgcctccgt
     5701 cgaccggccg acccgtcagg tattgatcga agcgcgtatt gtcgagggcg agcacgggtt
     5761 ttcgcgcaat ctcggcgtgc gcctttccat ggcagccgcc agcgctgacg gcaccgcccg
     5821 gggcttgatg ggcggcaagg acggcaccgt gtacgatctg tcggcccggc caatttccgg
     5881 ttttgacgcc gccactgccg ggctgacctt attcgcagcc ggggcgaccc ggctcctgaa
     5941 catcgaactg agcgcgctgg aggcggaagg gcgcggtgaa atcgtctcga gccctcgcgt
     6001 cgtgacagcg gaccggatga aggcggttgt cgagcagggt acggagctgc cgtaccaggc
     6061 gaaagtcggg cagggcgtgt cgggcgtgca gtttcgccgc gccacgctca aactggaagt
     6121 cgagccgcaa atcatgccgg acggcagggt ggtgctggat ctcgacgtcg ccaaagacag
     6181 cgtcggcgaa cagaccgacg ccgggcccgc gatcaacacg aaacacgtgc aaacgcgtgt
     6241 agaagtcgag gatggtggta cggtgtcgat cggcggaatt tacgcaaccg atgaccggga
     6301 cgatgtgacg cgggtgccag tcctgggcaa aatacccgtt ttaggcgcgc tttttcgcca
     6361 tcgggcccat cgggaccagc gcagtgaact ggctgttttc atcacgccgc gcgtcgttca
     6421 gaaaaattag gggcacgctg ccgccgctgt tcagcggcgg tctgcaggct cgacaaggca
     6481 gccgctttgc cagtaagctg cggcacgaac cataccggat tagccagagg acaccgttgc
     6541 aagcgcggga cgcacacgcc aatgtatttt ttgtagggct catgggggca ggcaaaacca
     6601 ccgtgggtcg ggccattgcg cgccgtctcg atcgcccgtt cttcgattcc gatcatgaaa
     6661 tcgaggcgcg tacgggcgcg cgcatcccgg tgatcttcga gctggaaggc gaagcgggct
     6721 ttcgcgatcg cgaggcgcac gtgatttccg acctgaccgg gcgcgagaat atcgtgctgg
     6781 ccacgggcgg cggcgcggtg ctgcggccgg aaaaccgcga agcgctgcaa aatcggggcg
     6841 tggtgatcta cttgcgcgcc aatccacatg atctgtggct gcgcacccgg cgcgacaaga
     6901 accgcccgct tttgcagacc gaagacccca aagcgcgcct cgaagcgctc tacgaagtac
     6961 gcgatcccct gtatcgggaa tgcgcgcact ttgtgattga aaccggccgg ccttcggtca
     7021 acggactcgt caacatggtt ctgatgcagc tcgagatggc cggcgtcgcc aaacatcctg
     7081 cgtcataatg gaccgtatga ttaccgtcaa tgtcgaactg ggcgagcgcg cctaccccat
     7141 ccatatcggt gccgatctga tcggccagac cgcgttgttc gcgccgcata tcgccggcag
     7201 ttcggtcacg atcgtcacga acaccaccgt cgagccgctt tacggcgacc aactgcgcgc
     7261 ggcgcttgcg ccgctcggca agcaggtctc cacggtcgt
//
LOCUS       DATASH010000431         4343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6594192,
            whole genome shotgun sequence.
ACCESSION   DATASH010000431 DATASH010000000
VERSION     DATASH010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6594192"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..281
                     /gene="fliQ"
                     /locus_tag="VHV99_11940"
     CDS             12..281
                     /gene="fliQ"
                     /locus_tag="VHV99_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HEX3636182.1"
                     /translation="MNQESVMTLAHQAMYVGLLLSAPLLLVALVVGLVVSLFQAATQI
                     NETTLSFIPKLLAIAITMVIAGPWMLTIMLDYLRQTLTNIPTLVN"
     gene            328..1110
                     /gene="fliR"
                     /locus_tag="VHV99_11945"
     CDS             328..1110
                     /gene="fliR"
                     /locus_tag="VHV99_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HEX3636183.1"
                     /translation="MFSVTYAQLNAWLTAFLWPFVRILALVASAPVLGNTSLPTRVKV
                     GLAAFITIIVAPTLGAMPQVTVFSAEGVWIIVNQFLIGIALGVTMQIVFQAISATGDF
                     VGLGMGLGFATFFDAQADSSSAVLSTYMNTIAMLVFLVIDGHLQMISALLATFQSVPV
                     AANILGAPGWRTLANFGSTVFSAGLLLSLPVVVALLITNLSLGILNRAAPQIGVFQIG
                     FPLTMLIGMLLLQLMIPNMIPFFMRLFDVGIDQMGRVAAGFR"
     gene            1337..2398
                     /locus_tag="VHV99_11950"
     CDS             1337..2398
                     /locus_tag="VHV99_11950"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HEX3636184.1"
                     /translation="MHRLSSPLRASACTSAPVKNPAWKRWLSAVLIPAALSACTKTDE
                     PVAVVPLDRGGAPPLLAVNVGERPYPLLFDTGATSIIVDPRTAAQHLKQLSDAEIAAW
                     ERYGEPLARTTTLLGGKEAVLQYYRAPSSRYGRWALPFTEVIQATRVPALDALSRKAG
                     ERVDGVLGMKQISTLNWMWDPTAGRLTGYRFASKAFAAQRDQMTCTAIRLQSDGTPTI
                     AILLDGWTMWFTLDTGFVGDASGSLSIADVAPLRHVNALSGEVTVHRAKSADGKAGES
                     VTVVQVKTAQLENLQLDGLVFHQIALSPARLGVSFINRFKRVAFDFETNRFCFVPPKQ
                     SMPDKLPTKAELAEYTVDN"
     gene            2528..3547
                     /locus_tag="VHV99_11955"
     CDS             2528..3547
                     /locus_tag="VHV99_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636185.1"
                     /translation="MMSKYKLTARCGRAKAGAGVLVMMIFSVAFSACTPNDDARVVIP
                     TEMKDVPLSLKLTSGPHSYRFLLSTVTLNTVVDQAMAKRDLKPLTPAECAVRECPDIV
                     NGESETPFKYYWLPQLQAGQWTIPSDFALAPFDYAEHAEEPGGRVDGVLGVTTIGTLN
                     WLWNRSAGTMSGYRADSRFFAAERSKMVCTAMEMLGGRAPAIQLELGGQPQWFEIDTA
                     MTGKLSGNLNRKHLAALRAGNGIAAEVMFTPKGRPAESGRHALVQPKSISLAGVPLDG
                     LAFDEIVAYGDSTLGVSFLQKLDNAAFDFGDHRFCIPASTRVKPDTLPTQAELERLAT
                     PTPHD"
     gene            complement(3647..>4343)
                     /gene="flgL"
                     /locus_tag="VHV99_11960"
     CDS             complement(3647..>4343)
                     /gene="flgL"
                     /locus_tag="VHV99_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490199.1"
                     /note="with FlgK acts as a hook filament junction protein
                     to join the flagellar filament to the hook; Yersinia,
                     Vibrio parahaemolyticus, Bradyrhizobium and other
                     organisms have 2 copies of this and other flagellar genes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HEX3636186.1"
                     /translation="VLAIFGSVASIGTSSVPAAANGNGGTGVISTVSLNNPTDPTNAD
                     TYTINFLTPTTYTVSQTSPATPGTPGTPGPVQTFTAGSTITLGGQSVNITGAPSSGDS
                     FTVTPATQGNMDVFANLNQLITTLQSPVAGAASGANLQNALTSSATQLENTINNVVTA
                     QANVGGREKEVQALQTVTQANTLQTSTNLTGLTQTNMVQTIAQYTMEQTTLQAAQLAF
                     SKIQNLSLFQYLN"
BASE COUNT          771 a         1392 c         1270 g          910 t
ORIGIN      
        1 gtcgactcgc catgaatcaa gaatccgtca tgacactcgc gcaccaggcc atgtatgtcg
       61 gcctgctgct ctccgcgccc ttgctgctgg tcgcgctcgt ggtcggtctg gtggtgagcc
      121 tgttccaggc cgcgacgcag atcaacgaaa ccacgctgtc gttcattccg aagctgctcg
      181 caatcgccat cacgatggtg atcgccggcc catggatgct gacgatcatg ctcgactacc
      241 tgcgccagac gctcaccaac atccccacgc tcgtcaactg agcgtggccc cggcctcggc
      301 tcggcgctcg ccccctcccc caccgtcatg ttttccgtca cctacgcaca actgaacgcc
      361 tggctcacgg cattcctgtg gccgttcgtg cggattctcg cgctggtcgc gagcgcgccg
      421 gtgctcggca acacgtcgct gccgacacgc gtgaaggtcg gcctggcggc tttcatcacg
      481 atcattgtcg cgcccacgct cggcgccatg ccgcaagtaa ccgtgttttc cgccgaaggc
      541 gtgtggatca tcgtcaatca gtttctgatc ggcatcgcgc ttggcgtgac gatgcagatc
      601 gtgtttcagg cgatcagcgc gaccggcgac tttgtcggcc tgggcatggg cctcggcttc
      661 gcgacattct tcgatgctca ggcggacagt tcgagcgcgg tgctgtcgac ctacatgaac
      721 acgatcgcga tgctggtgtt tctggtgatc gacggccatc tgcaaatgat cagcgcgctg
      781 ctcgccacct tccagtcggt cccggtggca gcgaacatcc tcggcgcgcc gggctggcgc
      841 accttggcga atttcggcag tacggtgttt tcggcgggac ttctgctctc gctgccggtg
      901 gtcgtcgcgc ttctcatcac caacctctcg ctgggcattc tcaaccgcgc cgcgccgcaa
      961 atcggcgtat tccagatcgg ctttcccctg acgatgctga tcggcatgct gctcctgcag
     1021 ctgatgatcc cgaacatgat tccattcttc atgcgtctgt tcgacgtcgg catcgatcag
     1081 atgggacgcg tggcggccgg cttcaggtaa cgcgacggcg gcgcggcact tcgccacctg
     1141 cacccatccc acctttttca gcattatctg aagcccgtca tcgtgctgct gccggtacat
     1201 tgacttttcc gctgcctgcc cgtggaacac gcttccggta cacccctgaa caccccaccg
     1261 atgcgatagg cagatgctgc ggctcaccgt ccgctctacg agcgggcttc gatcatgccg
     1321 ctttcaggga gcgcccatgc accgtctctc ttcccctctt cgcgcgtccg cctgtacatc
     1381 ggcgcctgtc aaaaatcccg cctggaagcg ctggctgagt gctgttctga tcccagccgc
     1441 gctcagcgcc tgtacaaaaa ccgacgagcc ggtagccgtc gtgccgcttg atcgcggtgg
     1501 tgcgccgccg ctgcttgctg tgaacgtcgg tgaacgcccg tacccgcttt tgttcgacac
     1561 cggcgcgaca tccatcattg tcgatccacg caccgcggca cagcatctca aacaactgag
     1621 cgacgcagaa attgccgcgt gggaacgata cggcgaacca ttggcgagga ccaccacgct
     1681 gctgggcgga aaggaagccg tgctccagta ctatcgggct ccatcctcgc gttatggacg
     1741 ctgggcctta ccgttcaccg aagtcatcca ggctacccgg gtacccgcgc tggacgccct
     1801 ctcgcgaaaa gctggagagc gcgttgatgg cgtcctcggg atgaaacaga tctcaacgct
     1861 caactggatg tgggatccga ccgcgggccg gctcacgggc taccgcttcg cttcgaaagc
     1921 gtttgccgca cagcgcgacc aaatgacctg cactgcaatc cgcttgcagt cagacggcac
     1981 cccaactatc gcgattctgt tagatggttg gaccatgtgg ttcacgctcg acacaggctt
     2041 cgtcggcgat gcgagcggtt cactgtccat tgccgacgtg gcgcccttgc gtcacgtcaa
     2101 cgcgttgagc ggcgaagtga cggtccatcg cgctaaaagc gccgatggca aggcgggcga
     2161 atcggtcact gtggtgcagg tcaagacggc acagctcgag aatctgcaac ttgacggtct
     2221 cgtctttcat cagatcgcgc tctcccccgc gcgcctcgga gtgagtttca tcaatcggtt
     2281 caaacgcgtc gcgttcgatt tcgaaacgaa ccgtttctgt tttgttccgc cgaaacagtc
     2341 catgcccgac aagcttccca ctaaagccga actggccgag tacacagtcg acaactgagt
     2401 cgaccggcgt tttcgctatg gccggtgtcc gatgcaccgc gcgatccgtc gcaacagacg
     2461 tgtccttata cgctgccgaa accgctcgcg tccgattggt tattcgcaat tgaagatgag
     2521 ggagtccatg atgtcgaaat acaaactcac cgcgcgttgt ggccgcgcaa aagctggagc
     2581 gggtgtactc gtcatgatga ttttcagcgt cgcattcagc gcgtgcacgc cgaatgacga
     2641 tgcgcgcgtc gtcattccaa ccgagatgaa ggacgtgcct ttgtcactca aactgacgtc
     2701 cggcccgcac agctaccgct tcctgctttc caccgtcacg ttgaacacgg tcgtcgacca
     2761 ggccatggcc aagcgcgacc tgaaaccgtt gacgcccgcc gaatgtgcgg tgcgtgagtg
     2821 ccccgacatt gtcaatggag agtccgaaac accgtttaaa tattactggc tgccgcaact
     2881 ccaagccggt cagtggacga ttccgtcgga cttcgccctc gccccgttcg actatgcgga
     2941 gcacgctgaa gaacctggcg gccgggtaga cggcgtactc ggcgtcacga caatcggcac
     3001 gctgaactgg ttgtggaatc gcagcgcggg cacgatgagc ggctaccgtg ccgattcgcg
     3061 attcttcgca gcggagcgca gcaagatggt gtgtacggca atggaaatgc tcggagggcg
     3121 agcacccgcc atccagctcg aacttggtgg tcagccgcag tggttcgaga tcgatactgc
     3181 catgacgggc aagctcagcg gcaatctgaa ccgcaaacac cttgccgcgc tgcgagcggg
     3241 caacggcatc gcggcggaag tcatgttcac gccgaaaggg cgcccggcgg agtcggggcg
     3301 ccacgcgctg gtgcagccga aatcgatatc ccttgcaggc gttccgctcg acggtctggc
     3361 gttcgacgaa atcgtagcct acggcgactc gaccctcggc gtcagtttcc tgcagaaact
     3421 cgacaacgcc gcgttcgatt tcggcgatca tcgcttctgc attcctgctt ccactcgcgt
     3481 gaagcctgac acgttgccga cgcaagccga actcgagcgt ctggccacgc caacgcctca
     3541 tgactgaaga atgaatgcaa ccccactgca cgagccgcat tagcaagaag ggcggcgccg
     3601 gttcgcaccg gcttccgccc ttcttgcgtt cagcatctct gtccgcttag ttcaggtact
     3661 ggaacagcga caggttctga atcttcgaaa acgccagttg cgccgcttgc agtgtggttt
     3721 gctccatcgt gtactgggca atggtctgga ccatgttggt ttgcgtcaga cccgtcaggt
     3781 tcgtcgacgt ctgcaacgtg ttggcctgcg tgaccgtctg caacgcctgc acttccttct
     3841 cgcggccacc tacgttcgcc tgcgcggtga ccacgttgtt gatcgtgttc tcgagctggg
     3901 tcgcactact ggtcagcgca ttctggagat tggcccccga agccgcgccc gcgaccggcg
     3961 attgcagtgt ggtaatcaac tggttcagat tcgcgaacac gtccatgttg ccttgagtgg
     4021 ccggcgttac cgtgaagctg tcgcctgagc tcggtgcgcc ggtgatgttc accgactggc
     4081 cgccgagcgt gatggtcgaa cctgccgtga acgtctgcac cgggcctggt gtgcctggcg
     4141 tgcccggtgt tgccggactg gtctggctca ccgtgtaggt ggtcggtgtc aagaaattga
     4201 tggtgtaggt gtcggcattg gtcggatcgg tcggattgtt cagactcacc gtgctgatca
     4261 cgcccgtgcc gccgttaccg ttcgctgcgg ccggcacgga actggtgccg atcgacgcca
     4321 cgctgccgaa aatcgcaagg acg
//
LOCUS       DATASH010000432         3673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1650613,
            whole genome shotgun sequence.
ACCESSION   DATASH010000432 DATASH010000000
VERSION     DATASH010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1650613"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..1014)
                     /locus_tag="VHV99_11965"
     CDS             complement(127..1014)
                     /locus_tag="VHV99_11965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HEX3636187.1"
                     /translation="MRLGAAPVLASPGGGAAARAAQAIERLAQARGFIVDLDGTLVAS
                     GQALPGAARLLAYGGERCVIVSNNSRDTARSLSRKLKRIGLRITPEHIVLAGEQAVLM
                     VARRYPGARIRLAASATLAAFARRQGCVLVEHAPDVVILGRDVHFNYAKLAAIVNDVR
                     GGARLIVTNPDLSHPTNGGGLVPETGSLMRAVIGCAGVAPEQIVGKPEQPLFIEALQR
                     LGTPAASTLVIGDNPATDAAGAARLGMPLVLVGASECADVASLAALFPSEGGEQAQCR
                     RSSMALQEQGVECIGDRCD"
     gene            1351..2226
                     /gene="phnC"
                     /locus_tag="VHV99_11970"
     CDS             1351..2226
                     /gene="phnC"
                     /locus_tag="VHV99_11970"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HEX3636188.1"
                     /translation="MDGVSMEAIRVERLSKTFRNGRKDLDEIDLRVAPGEMVALIGAS
                     GSGKSTLLRHIAGFTVSDAQPSQISILGRPIQQNGRLVREVRRIRRDIGFVFQQFNLV
                     NRLSVESNVLIGALARLPWWRRVTGRFPSSERALSAAALGEVGIAEHAQERAANLSGG
                     QQQRAALARALVQRARIVLADEPIASLDPESSRRVMDMLRTLNRDHGLTVLVSLHQVD
                     IAMQYCPRTVALRRGKIVYDGASSALTPALLQQLYGDDARELLEEIHHGAAQQHADAP
                     QSQRYSFALDPAHSA"
     gene            2277..3242
                     /gene="phnD"
                     /locus_tag="VHV99_11975"
     CDS             2277..3242
                     /gene="phnD"
                     /locus_tag="VHV99_11975"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018434192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX3636189.1"
                     /translation="MKFLRSLIAGAAAFACVAAHAEDINLGIISTDSSAVLSQRWQPL
                     VDDMSKQTGLHVKAFFATDYAGIIEGMRFNKVQVGYFGNASAMEAVDRADGEVFAKIT
                     YANGDSGYKSVLITNVNSRFKTLDDVFKNTKDVTLGFGDPNSTSGTLVPGYYLFAQHN
                     APVNTSFKAVLPSNHEANLLAVVNNKIDIATNNTEMLDTLKKDHPDKFAQVRVLWTSP
                     LIASDPLVWRKDLSQGTKDKIRTFFLNYGKTDPHEKAVMAAITGYSGFSASSDAQLLP
                     TRQVSLFQQKQKIQSDTHLSDDDRKTQLAAIDAKLSALNATLSKQ"
     gene            3239..>3673
                     /locus_tag="VHV99_11980"
     CDS             3239..>3673
                     /locus_tag="VHV99_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HEX3636190.1"
                     /translation="MSASDLTTARPTPPVGALAGATVGKRSWLSLLGWVGVIAVLGGS
                     WHSADMRPLDLLTDSGNMGQFAKDFFPPDFSEWRNYVHEMVVTLSVAVWGTALSLVCA
                     VPCGLMCARNMAPQWVVQPVRRLMDACRAINEMVFAMLFIVAV"
BASE COUNT          677 a         1178 c         1132 g          686 t
ORIGIN      
        1 atggggttga actcggcgag ctgaggaaaa aaggcgtcgt ttgacacacg cccgatgcat
       61 gaagctcgat caggaatcga tcgtcgattc atgcgctttg tgtcgtgcgc atccggctac
      121 gggagattaa tcgcaacggt ccccgataca ttcgacgccc tgttcctgaa gcgccattga
      181 agagcgcctg cattgcgcct gctccccgcc ttcggacggg aacagcgccg ccagcgatgc
      241 gacatccgcg cactcgctgg cgccgaccag caccagcggc ataccgagcc gggccgctcc
      301 ggccgcgtcg gttgccgggt tgtcgccgat gaccagcgta ctggccgctg gcgtaccgag
      361 ccgctgcagc gcctcgataa acagcggctg ttcgggctta ccgacgatct gctcaggtgc
      421 cacgccggcg cagccgatca ccgcgcgcat caggctgcca gtctcgggca ccaggccgcc
      481 gccgttcgtc ggatggctca ggtcagggtt ggtcacaatc aggcgcgcgc cgccgcgcac
      541 gtcgttgacg atcgccgcga gtttcgcata gttgaagtgc acgtcgcggc cgaggatgac
      601 gacgtccggg gcatgctcga ccaggacgca cccttgacgc ctcgcgaagg ctgcgagcgt
      661 cgcgctcgcg gcgagtcgaa tccgcgcgcc cggataccgc cgggcgacca tcagcaccgc
      721 ctgctcgccc gccagtacga tgtgctccgg tgttatccgc aggccgatcc gcttgagctt
      781 gcgcgacaga ctgcgcgcgg tgtcgcgcga attgttcgat acgatcacgc agcgctcgcc
      841 gccataggcg agcagccgcg ccgcgcccgg tagcgcctgc ccgctcgcga ccagcgtgcc
      901 gtccagatcg acgatgaaac cgcgtgcctg cgccagccgc tcgatcgcct gcgcggcacg
      961 cgcggccgca ccgccgcctg gtgaggcaag gaccggagcg gcgccgaggc gcactgagtc
     1021 gtcggcggac ggcacggacg gaaaactcat catgcgtatc cctttgagcc gaaagaaaag
     1081 ctcgcaagcc taatattgaa tttatatcaa tgcaattctt ttatgcattg agagatagtc
     1141 atttcattga cacttgggag gactatgctt gcgccacaat gcagcggctt gagagcctcg
     1201 ccaacttttg cacgctctcc tatccgaagc cacgcgttgc ggcactgata cggattgaaa
     1261 gacatagtct tctagacgtt gagatagctt cacttaactg tcacggtggc ttcctagaat
     1321 gtggcacacg ttccggcact tgccggatgc attgacgggg taagcatgga agcaattcgc
     1381 gtcgaacggc taagcaagac attcagaaac ggccgcaagg atctcgatga aatcgatctg
     1441 cgcgtcgccc cgggcgaaat ggtggcgctg atcggtgcct cgggctcggg caaatcgacg
     1501 ctgctgcggc acatcgcggg tttcaccgtc tccgacgcac agccgtcgca gatttcgatt
     1561 ctcggccgcc caatccagca gaacggccgc ctcgtgcgtg aagtgcggcg gattcgccgc
     1621 gatatcggct tcgtgttcca gcagttcaac cttgtcaacc ggctgtcggt cgagtccaac
     1681 gtgcttatcg gtgcgcttgc gcggctgccg tggtggcgcc gcgttacggg ccgtttccca
     1741 agcagcgaac gggcgctgtc ggctgccgcg ctcggcgagg ttgggattgc tgaacatgcg
     1801 caggaacgcg cggcgaacct ctccggcggc cagcagcaac gcgcggcgct tgcacgtgcg
     1861 ctggtgcagc gggcccgcat cgtcctcgcg gacgaaccga ttgcgtcgct cgatcctgaa
     1921 tcgtcgcgcc gcgtgatgga catgctgcgt acgctgaacc gcgatcacgg gttgaccgtg
     1981 ctggtgtcgc tgcatcaggt cgacatcgcg atgcagtact gcccgcgtac cgtcgccctg
     2041 cgccgcggca agatcgtcta cgacggagcc tcttccgcgc tcacgcccgc tttgttgcag
     2101 cagctttacg gcgacgacgc tcgcgaactg ctcgaagaaa ttcatcacgg cgctgcgcag
     2161 cagcatgccg atgcacccca gtcgcagcgc tactcctttg cactcgatcc tgcgcactct
     2221 gcctgaaata cagcgtcgct cccattcctt ctgttctgcc aactggatta aaaccgatga
     2281 aattcctgcg ctcactgatc gccggcgccg ccgcatttgc ttgcgtcgcc gcccatgccg
     2341 aagacatcaa tcttggcatc atctcgaccg attcgtccgc ggtgctgtcg cagcgctggc
     2401 agccgctggt cgacgacatg agtaagcaga ccggtttgca cgtgaaggcg ttcttcgcca
     2461 ccgactacgc cggcatcatc gagggcatgc gctttaacaa ggtgcaggtc ggctacttcg
     2521 gcaacgcctc ggcgatggaa gctgtggatc gcgccgacgg cgaggtgttt gccaagatca
     2581 cctatgcgaa cggcgactcc ggctacaagt ccgtgctgat caccaacgtg aacagccgct
     2641 tcaagacgct cgacgacgtc ttcaagaata ccaaggatgt gacgctcggc ttcggcgatc
     2701 cgaattccac gtcgggtacg ctggttcccg gctactacct gttcgcccag cacaatgcgc
     2761 cggtgaatac ctcattcaag gcggtgctac cgtctaacca tgaagcgaat ctgctggctg
     2821 tagtgaacaa caagatcgac attgcgacca acaacaccga gatgctcgac acactgaaaa
     2881 aggatcatcc ggacaagttc gcccaggtgc gcgtactgtg gacctcgccg ctgatcgcat
     2941 cggacccgct ggtctggcgc aaggatctgt cgcagggcac caaggacaag atccgcacct
     3001 tcttcctgaa ctacggcaag accgacccgc acgaaaaggc ggtgatggcc gcgattaccg
     3061 gctatagcgg cttctcggcg tcgtccgatg cgcaactgct gccgacccgc caggtctcgc
     3121 tgtttcagca gaagcagaag attcagagcg acacgcatct gtccgacgac gaccggaaga
     3181 cccagctcgc cgcgatcgac gccaagctga gcgcgctgaa cgcgacttta tcgaagcaat
     3241 gagcgcgtcc gacctgacta ccgcccgccc gacgcccccg gtcggcgcgc tggcgggcgc
     3301 tactgtgggc aagcgcagct ggctgtctct gctaggctgg gtcggcgtga tcgccgtgct
     3361 ggggggaagc tggcacagcg cggatatgcg gccgctcgat ctgctgacgg actccggcaa
     3421 catgggccag ttcgccaagg acttcttccc gccggacttc agcgagtggc gcaactacgt
     3481 gcacgaaatg gttgtgacgt tgtcggtcgc ggtgtggggc acggctctct cgctggtatg
     3541 cgcggtgccg tgcggtctga tgtgtgcgcg caacatggcg ccgcagtggg tcgtgcagcc
     3601 ggtgcgccgc ctgatggatg cgtgccgcgc gatcaatgaa atggtcttcg cgatgctgtt
     3661 catcgtcgcg gtg
//
LOCUS       DATASH010000433         7328 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_858713,
            whole genome shotgun sequence.
ACCESSION   DATASH010000433 DATASH010000000
VERSION     DATASH010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7328
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858713"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VHV99_11985"
     CDS             <1..232
                     /locus_tag="VHV99_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004527638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX3636191.1"
                     /translation="GTAAVEITSIMKRNSCGKALDIARLARDMLGGNGISDEFGIARH
                     LVNLEVVNTYEGTHDIHALILGRAQTGIQAFF"
     gene            complement(401..1039)
                     /locus_tag="VHV99_11990"
     CDS             complement(401..1039)
                     /locus_tag="VHV99_11990"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003265121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX3636192.1"
                     /translation="MSLRLGDIAPDFEQESSVGRIKFHEWLGDSWGVLFSHPADFTPV
                     CTTELGLTAKLAGEFEKRNVKTIALSVDSAESHKEWIKDINETQAANVGFPILADSDR
                     KVAELYDMIHPNANETLTVRSLFVIDPKKKVRLIITYPASTGRNFDEVLRVIDSLQLT
                     DSHSVATPGNWKQGDDVVIVPSLKDEEVIKQKFPKGYKALRPYLRMTPQPNK"
     gene            1397..1699
                     /locus_tag="VHV99_11995"
     CDS             1397..1699
                     /locus_tag="VHV99_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HEX3636193.1"
                     /translation="MSEVNKERLMSDIKTVLADAEDLLKQAASATGERASELRETALT
                     RLKQAKEKAADVQVVVVEKGKKAARATDDYVHEHPWASIGIAAGAGVLLGLLINRK"
     gene            1859..2245
                     /locus_tag="VHV99_12000"
     CDS             1859..2245
                     /locus_tag="VHV99_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX3636194.1"
                     /translation="MTIETQSQHGEHSPLRRIFGSVFALLQTRLELVGIELAEEKDRL
                     LGVLFLGLAAMMLATMALIALTALIAIAFWDTYRWQALASITAVYAIAGIACALKARS
                     GLRNAPMVFEATIAEFEKDRDVFRKP"
     gene            2371..2850
                     /locus_tag="VHV99_12005"
     CDS             2371..2850
                     /locus_tag="VHV99_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3318 domain-containing protein"
                     /protein_id="HEX3636195.1"
                     /translation="MSSTHSDTAFRNKRHQAKDLSTPHLRALRKELLLVRADVERMEL
                     AQATIELRQAVTHFSWLKFILPGFGGMRVGRGSKTSFINAGSIGALLKQYPLISSIAS
                     LVLAKPLRATIAAGAKPALKWGSLALAGWEAYRIWQQMKNESAASAGKKDEAVDSGY"
     gene            complement(2922..3353)
                     /locus_tag="VHV99_12010"
     CDS             complement(2922..3353)
                     /locus_tag="VHV99_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEX3636196.1"
                     /translation="MTRLHTSAFTLLELMIALAIAATLFGFAVPSYRSQVARTHRVDA
                     ASALYRAAQFVEGAASDGTAALPPGLDQAPQFGTPVYRLHLLPVDDTNGGYSLEAAPI
                     ETGPMRDDACGVFTLDATGLRGNRSSANGVAPANGECWNTS"
     gene            complement(3364..3810)
                     /locus_tag="VHV99_12015"
     CDS             complement(3364..3810)
                     /locus_tag="VHV99_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636197.1"
                     /translation="MMLTTSAAWFESSLAAARGANNVRDHLQAFHAADSALTLCARSV
                     VAAATVEPEPVASLPSGEPTQWKHEAAFEAGAVAPIAQWPGSLRPPQCLIEAWRLVTR
                     ADARAYLLTSRGFGRTKESQVWLQMELVITGEQIERHWRHIAARPF"
     gene            complement(3939..4748)
                     /locus_tag="VHV99_12020"
     CDS             complement(3939..4748)
                     /locus_tag="VHV99_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HEX3636198.1"
                     /translation="MTQRIYIARGHTLVEFVIAIALGLVVTAGAVSLYTTQRHVFERS
                     NDAMRIREAGLTALTLMGQQLQMAGFVPADVVRFTNPPPLFGCSGSRPTGADDYLACE
                     ALPSHSDGIAVRYVGDNVSTWPSATGQSTDCLGQAVTNNDAALGGQGVLVVNRYFARV
                     SGSTGEPELYCEGNGKAGSAQPLVEGVERVRLRYWLAGGLSAIDASAIAAAQWDRVVA
                     VDLCVLVRGAPQGRSTRYADCDGVSAMATDMRARQAFSRRVAIRNHAEDSL"
     gene            complement(4745..5203)
                     /locus_tag="VHV99_12025"
     CDS             complement(4745..5203)
                     /locus_tag="VHV99_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636199.1"
                     /translation="MSRYSSNPWARRCPARQSTTHRGSSLIEVMLAVALMAVTALGLL
                     AGQLWTAREARAMAMREHAAWIADSVAEAMRESSAGDAAIRQWSARATALLPHGEASV
                     GERSGVSTASVTWRALRDMPRADDVIDKPESCGGADVPTGTSCVALAFAK"
     gene            complement(5200..5760)
                     /locus_tag="VHV99_12030"
     CDS             complement(5200..5760)
                     /locus_tag="VHV99_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HEX3636200.1"
                     /translation="MKWDAVCLRPRRGFTLVETLAVVALLAIIAVMATPSFVAWHLRD
                     QIDARAKALASTLAYARSEAMRRGARVTVCRIDAARHCLAAGQACGNGVTDWSCGWAV
                     LAERGGTLSLLRAHPLLAAVSIIGTQTNLTFTPPAGQLIGTFRSFDIAPRVPSKATQG
                     DKWRRCIRIAAGGRARIVEGACGAVS"
     gene            complement(5965..6447)
                     /gene="nrdR"
                     /locus_tag="VHV99_12035"
     CDS             complement(5965..6447)
                     /gene="nrdR"
                     /locus_tag="VHV99_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006476792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEX3636201.1"
                     /translation="MHCPFCRHADTQVVDSRVSEDGATIRRRRRCPACDKRFTTYERV
                     ELALPSVVKKDGSRTEFDRRKIVASMQLALRKRPVAADAIDAAVARIEYQLLGSGERE
                     VRSERLGELVMNELRALDTIAYVRFASVYRRFEDVSEFEDVIEEFRRASSPPKPPRKR
                     "
     gene            complement(6454..>7328)
                     /locus_tag="VHV99_12040"
     CDS             complement(6454..>7328)
                     /locus_tag="VHV99_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011356345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636202.1"
                     /translation="VPLADFVTTTTHKSLRGPRGGIIICKERFAKGIDGQVFPGIQGG
                     PLEHVIAAKAVCFYEALQPSFKGYAQQIVSNARALAAGLIAAGVYPNPVPHADFVTTT
                     THKSLRGPRGGVILMKAEFEKQINSAIFPGIQGGPLMHVIAAKAVAFKEALSPEFKAY
                     QQQVVENARVLAETLVKRGLRIVSGRTESHVMLVDLRAKKITGKAAEAALGAAHITVN
                     KNAIPNDPEKPFVTSGIRLGSPAMTTRGFGVKEAEQVGNLIADVLDNPEDAATIERVR
                     AQVAELTQRFPVYG"
BASE COUNT         1396 a         2316 c         2225 g         1391 t
ORIGIN      
        1 aggcacggcc gccgtcgaga tcacgtcgat catgaaacgc aattcgtgcg gcaaggcgct
       61 ggacattgcg cggctcgcgc gcgacatgct cggcggcaac ggcatctcgg acgagttcgg
      121 gattgcgcgc catctggtca atctggaagt cgtgaacacg tacgaaggga cgcacgacat
      181 tcacgcactg attctcgggc gcgcgcaaac gggcattcag gcgtttttct gatgcgctga
      241 ttgcctggtt gcggcgtttc gtctcagccg ctgaatagca aatcgaaagt agacgttgaa
      301 aggccggttc aatctttgaa ccggcctttt ttcgttgcgg cgttgcggcg tcacggcgtc
      361 gcattgcagc taacggcgcc tttcttgacc catccgccgc ttacttgttc ggctgcggcg
      421 tcatccgcag atacgggcgc agcgccttat agcccttcgg gaatttctgc ttgatgactt
      481 cttcgtcctt caacgacggc acgatcacca cgtcatcgcc ctgcttccag ttgccgggcg
      541 tggccaccga gtggctgtcg gtgagttgaa gcgagtcgat cacgcgcagc acttcgtcga
      601 aattgcgccc ggtgctggcc ggataggtaa tgatcaaacg caccttcttc ttcggatcga
      661 tcacgaacag cgaccggacc gtgagggttt cattggcgtt cgggtggatc atgtcgtaca
      721 gctcagccac tttgcgatca ctgtcagcga gaatcggaaa gccgacgttg gcggcctggg
      781 tctcgttgat gtccttgatc cattccttgt gcgactcggc gctgtcgacc gacaacgcga
      841 tcgtcttcac attgcgcttt tcgaattcgc cggccagctt cgccgtcaaa ccgagttcgg
      901 tcgtgcaaac cggcgtgaag tcagcgggat gcgagaacag cacgccccaa ctatcgccca
      961 gccattcgtg gaatttgatg cggccgacac tcgactcctg ttcgaaatcc ggtgcaatgt
     1021 cgccaagacg taaactcata atgcagctcc ttgttgtatt cggacattct gcgcgagcat
     1081 tcgcgcgcga cgctctgctc aagcgcgctt cgccaaatta cagcatatgg gagcgacggt
     1141 acatatcgaa ccaacttcgc gtcactactt tatgcgattt tgtaattttc accgatttag
     1201 tggaaacttt gcgggacaga tcaactccat cttaggcaaa ctctgctgta gggttagcag
     1261 atgaagagat ttcaaagcct tacttcaacc gggaacggtt cgtgcggttg ttttcaacga
     1321 accgagcact gcgctcgcca gcggccgttt ctttggttac gattcacgcg tggcgtgaga
     1381 cgaaggggag ctgtcaatgt cggaagtcaa caaggagaga ttgatgtcgg atatcaaaac
     1441 cgtcctcgcg gacgctgaag atttgctgaa acaggccgcg agcgccaccg gcgagcgcgc
     1501 ttcggaactg cgtgaaacgg cgctcacgcg cctgaagcag gctaaggaaa aagcggctga
     1561 cgtgcaggtc gtggtggtcg aaaagggcaa gaaggccgca cgcgctaccg acgactacgt
     1621 gcatgagcat ccatgggcat ccatcggcat cgccgctggc gccggcgtgc tgttggggct
     1681 gttgatcaac cgcaagtaac actctggtga cgctgccggg cgaagcgtgc ccggcagacc
     1741 agcgaatcga gttgaccggc gcacgcctgg ccggtccgct tctcttggcg cgctcggctt
     1801 ttcttggcgc gctcgttttc acgcgccggt taggccttca cgcgcaacgc tcaaagccat
     1861 gacgatcgaa acacaatcgc agcacggcga acacagtccg ttgcgccgta tttttggatc
     1921 cgtgtttgct cttctgcaaa cgcggcttga actggtcggc atcgaactcg ccgaagaaaa
     1981 agaccgtctg ctcggcgtgc tgtttctcgg cctcgcggcc atgatgctcg cgacgatggc
     2041 cttgatcgca ttgacggcgc tgatcgccat cgcgttctgg gacacctacc gatggcaagc
     2101 gctcgccagc atcacggcgg tgtacgccat cgcgggaata gcctgtgcgc taaaagcgcg
     2161 cagcggtttg cgcaacgcgc caatggtgtt cgaagccacg attgccgagt tcgagaaaga
     2221 tcgcgacgtg ttccgcaaac cgtgaagccg cgtgtataac cggctcatgg gcgtgcttca
     2281 accgcccggc gcctttgtaa atgctcgcag acacttcgac cgatttgcag catagtttta
     2341 ctgccccacc tccactgccg ccgacacgcc atgagctcaa cccattccga taccgcattc
     2401 cgcaacaagc gccaccaggc caaagatctg agtacgcctc acctgcgagc gctgcgcaag
     2461 gaattgctgc tggtgcgcgc ggatgtcgag cgtatggaac tcgctcaggc gacgatcgaa
     2521 ttgcggcagg cggtcacgca cttcagctgg ctcaaattca ttctgccggg cttcggcggc
     2581 atgcgcgtgg gcaggggctc gaagacgagt tttatcaacg caggcagcat cggcgcgctc
     2641 ctcaaacagt atccgctcat cagttcgatc gcttcgctgg tgttggccaa gccactgcgc
     2701 gcgactattg cagcgggtgc gaagcctgcg ctcaaatggg gcagccttgc gcttgccggc
     2761 tgggaagcgt atcgaatctg gcaacagatg aagaatgaat ccgcggcgtc cgccggcaaa
     2821 aaagacgagg cggttgatag cgggtactga agcccgggta gtacgcgccc acgtatcagg
     2881 ttcatcgtct ggcattgccg cgccatgtcc aacagcgctc cctagcttgt gttccagcac
     2941 tcgccatttg ccggcgccac gccgtttgcg ctgcttctat tccctcgcag ccctgtcgca
     3001 tccagggtga aaacgccgca agcgtcgtca cgcatggggc cggtttcgat cggcgctgcc
     3061 tctaacgaat agccgccgtt tgtgtcgtcc accggcagca ggtgcaaccg gtacaccggc
     3121 gtgccgaatt gcggcgcctg atccagaccc ggcggcaatg ccgcggtgcc atcgcttgcc
     3181 gccccctcga cgaattgcgc tgcccgataa agggcggacg ccgcgtcgac cctgtgtgtt
     3241 cgcgcaactt gactccggta cgaaggcact gcaaatccga acagcgtggc agctatcgcc
     3301 agcgcgatca tcagttcgag tagcgtaaat gcggatgtgt gcaatctcgt cataaatctc
     3361 ccgtcaaaat gggcgtgcgg cgatatgacg ccaatgacgc tcgatctgtt cacctgtgat
     3421 cacaagttcc atctgcaacc acacctgtga ctctttggtt cggccgaagc cacgggaagt
     3481 caacaaatag gctcgggcat cggcgcgcgt gacaagacgc cacgcttcga tcaaacattg
     3541 cggcgggcgc aacgatccgg gccattgcgc gataggcgcg acagcgccgg cctcaaaagc
     3601 ggcttcgtgc ttccactgcg tcggctcgcc ggatgggagc gatgccacgg gctccggctc
     3661 gacagttgcc gccgcgacca cgctccgggc gcacagggtc agcgccgaat ccgcggcatg
     3721 aaatgcctgc aaatggtcgc gcacgttatt ggcgccccgc gccgcggcaa gcgagctttc
     3781 aaaccaggcc gcggaggtgg tcaacatcat cgcggaaatc agcaggacga tcggcaggac
     3841 ggtgccgctg ttgtgaacgc gcaatctccg aacggcatga ccatcgggat ttcgccgagc
     3901 cgatgccgca cggatcgttg tactgcaaag tccgtctgtc acagagaatc ctccgcgtga
     3961 ttgcgtatcg ccactcgccg cgagaaggcc tgccgcgccc gcatgtcggt agccatagcg
     4021 ctcacgccgt cgcaatcggc atagcgcgtg ctccgtcctt gcggggcccc gcgaacgagg
     4081 acacaaagat cgacggcgac gaccctatcc cattgagcag ccgctatcgc cgacgcgtct
     4141 atcgcgctca gcccgccggc tagccagtac ctgagtcgta cgcgttcgac gccttcgacc
     4201 agcggttgcg cggaccccgc cttgccattg ccctcacagt aaagttccgg ctcgcccgtc
     4261 gacccgctga ccctcgcgaa gtatcggttg accaccaaca cgccctgccc gccgagtgcc
     4321 gcatcgttgt tcgttacggc ctggccgagg cagtcggttg attggccggt cgctgacggc
     4381 cacgtcgaaa cgttgtcacc gacataacga accgcgatac cgtccgagtg gctcggcaac
     4441 gcctcgcagg cgaggtagtc gtccgcaccg gtcgggcgac tgcccgaaca accgaacaaa
     4501 ggcggtggat tagtgaacct cacgacgtcc gcgggtacaa agccggccat ttgaagttgc
     4561 tggcccatca gcgtcagtgc ggtcaggccg gcttcgcgga tccgcattgc gtcgttcgag
     4621 cgctcgaaca catgacgctg cgttgtataa agcgagaccg cgccggcagt cactaccagc
     4681 cccaacgcaa ttgcgatcac gaactcgacg agcgtgtggc cacgcgcgat atagatgcgt
     4741 tgcgtcattt tgcgaacgcc agcgcaacgc atgaggtgcc agtgggaaca tccgcaccac
     4801 cgcacgactc gggcttgtcg atgacatcgt cggcgcgcgg catgtcgcgc agagccctcc
     4861 acgtgacact tgcggtggac acgccgctgc gttcccctac cgaagcctcg ccatgaggca
     4921 gcagggccgt tgcacgcgcg ctccattgtc tgatcgcagc gtcgccggcc gatgactcgc
     4981 gcatcgcttc ggcgacggaa tcagctatcc atgcggcgtg ctcgcgcatc gccatcgcac
     5041 gggcttcgcg cgcggtccac aattggccgg cgagcagccc aagcgccgtc actgccatca
     5101 gggcgacggc cagcatcact tcgatcagcg aactgcctct atgcgttgtt gactgccttg
     5161 ctgggcaacg gcgtgcccac gggttcgacg aatatcggct cacgataccg ctccgcacgc
     5221 gccctcgaca atccgcgcac gcccgcccgc cgcgatgcgg atacaccgcc gccacttgtc
     5281 gccctgggtc gctttcgacg gcacgcgtgg ggcgatatca aagctgcgaa aggtgccgat
     5341 caactgcccc gcgggcggcg tgaacgtgag attcgtctgc gttccgatga tactgaccgc
     5401 cgccagtaaa ggatgcgcgc gcagtagcga gagcgtgcca cctcgttcgg ccaatacggc
     5461 ccagccgcac gaccagtcgg tgaccccgtt gccgcacgct tgcccggcgg caagacaatg
     5521 ccgcgccgcg tcgattcggc acacggtgac gcgagcgccg cggcgcattg cttcactgcg
     5581 cgcataggcc agcgttgagg caagcgcttt cgcgcgtgcg tcaatctggt cccgcaagtg
     5641 ccatgcgacg aacgacggcg tcgccatcac cgcgattatc gccagcagcg cgacaacagc
     5701 cagtgtttcg acaagcgtaa agccgcggcg cggcctcaaa cagactgcat cccatttcat
     5761 ctgcatccct gatcgtttca aagaatgcag ccaatctagc gattcgcaaa cgggtcgaca
     5821 atcggccgaa tggccaggtg gcactcagac gctatcccgc gcaaaaatgc acgcgtgacg
     5881 aacataacgg gagcggagat gcggatgcgc gaacttacgc ggaagaaaac agagcctgga
     5941 gcgcaacttc aggcgcgaca gcaatcagcg cttgcgtggc ggcttgggag gagaagaggc
     6001 gcgccggaat tcttcgatca cgtcctcgaa ttcggagacg tcttcaaagc gccggtagac
     6061 agaggcgaaa cgaacataag caatggtatc gagcgcacgc aactcgttca tcacgagttc
     6121 gccgaggcgc tcgctgcgca cctcgcgctc gccgctgccg agcagttgat attcgatgcg
     6181 cgcgactgcc gcgtcgatcg cgtccgctgc taccgggcgc ttgcgcagcg ccagttgcat
     6241 gctcgcgacg atcttgcggc gatcgaactc cgtgcggctg ccgtccttct tgacgaccga
     6301 cggcaacgcc agctcgaccc gctcatacgt cgtgaaacgc ttgtcgcagg ccgggcagcg
     6361 gcggcgccgg cgaatcgtcg cgccgtcttc ggatacgcgc gagtcgacga cctgcgtatc
     6421 ggcgtggcgg cagaaggggc agtgcatgac ggcttagccg taaaccggga agcgctgggt
     6481 cagctcggcg acctgcgcgc gcacccgctc gatcgtcgcg gcgtcttccg ggttgtccag
     6541 cacgtcggcg atcaggttgc cgacctgctc ggcttccttc acgccgaagc cgcgcgtggt
     6601 catggccggc gaaccgaggc ggatgcccga ggtcacgaac ggcttttccg gatcgttcgg
     6661 gatcgcgttc ttgttgaccg tgatgtgcgc cgcaccgagt gccgcttccg cagccttgcc
     6721 ggtgatcttc ttcgcgcgca ggtcgaccag catcacgtgg ctttccgtgc ggcccgacac
     6781 gatgcgcaga ccccgtttga ccagcgtttc agcgagcacg cgcgcgttct cgacgacctg
     6841 ctgctgatac gccttgaatt ccggcgacaa cgcttccttg aacgcgacgg ccttcgcggc
     6901 gatcacgtgc atcagcggac cgccctggat gcccgggaaa atcgccgagt tgatctgctt
     6961 ttcgaactcg gccttcatca ggatcacgcc gccgcgcggg ccacgcaggc tcttgtgcgt
     7021 ggtggtggtg acgaaatcgg cgtgcggcac cgggttcgga taaacgcccg cggcgatgag
     7081 gccggcggcc agggcgcgcg cgttgctcac gatctgctgg gcgtagccct tgaaggaggg
     7141 ctgcaacgct tcgtaaaagc acacggcttt ggcggcgatg acgtgttcga gcgggccgcc
     7201 ctggatgccg gggaagacct gcccatcgat gcccttggcg aatcgttcct tgcaaatgat
     7261 gatgccgccc cgcggcccac gcaggctctt gtgcgtggtg gtggtgacga aatcggcgag
     7321 cggcacgg
//
LOCUS       DATASH010000434         5881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3825772,
            whole genome shotgun sequence.
ACCESSION   DATASH010000434 DATASH010000000
VERSION     DATASH010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5881
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3825772"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="VHV99_12045"
     CDS             complement(<1..723)
                     /locus_tag="VHV99_12045"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX3636203.1"
                     /translation="MAKQAGRRSFLASSGTLGLGACLAVCAPAHAADATEDTLDKIRR
                     TGVFNIGLREAAPPYSFKNTSGQYVGFSTEIANAIYQAISHELGGNIKVNYVPLTSAT
                     RIVLLQNGTIDIEAGATVMTLSRAKVVDFSIPHFVTATGLLVRADSPIKSVADLAGRR
                     VGIPQGGLESIMYQDAVTQGKIKGAVRTLAFPDHAQGTTALQTGMIDGYSTDNPILYQ
                     IASSTTGLRVVPLNLNAASQTML"
     gene            complement(843..1571)
                     /locus_tag="VHV99_12050"
     CDS             complement(843..1571)
                     /locus_tag="VHV99_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX3636204.1"
                     /translation="MISLQSVSKWYGQHKVLSDCTLNVEKGEVVVVCGPSGSGKSTLI
                     RCVNGLEEIQQGVITVDGCKVTPRQKDLLKLRRKISMVFQSFELYPHKKVIDNLTLAP
                     VVALGKDRSDAEHYARELLERVGMAAFAERWPGNLSGGQQQRVAIARALAGDPIAVLF
                     DEPTSALDPEMINEVLEVMANLAKTNMTMIVVTHEMGFARRVADRIVFMESGVIIEDT
                     PADTFFRQPKSERAARFIEKILSH"
     gene            complement(1623..2894)
                     /locus_tag="VHV99_12055"
     CDS             complement(1623..2894)
                     /locus_tag="VHV99_12055"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX3636205.1"
                     /translation="MLRDGLRGVPPGQSGVRSDEVAQFDWHPFDGKMSLPLLSLDLDA
                     YSGNRSAMIKICQHFGISIAPHAKTPMSPALAADLVENGAWAISVADIRQAAIMLDSG
                     LTRIVIANEIGGRNAIKRLAHLLSRFPNAEVHVFVDSEDFVEALDSVWRQDANLPALG
                     LLLEVGCGRGGVQTQAQVDGIVRAVERCDNERLSFSGVATYEGTANTPDAAQLKINLD
                     LLFQRVEAALTAVRKSVAPNKRLILTAGGSSLFDHVITHSLPIIAADRDCVLVLRCGA
                     VFFCDHGPISARLQAMAQRGLLGDAVTDVIAHAFKPTLRIWAEVLSVHDDVAICGLGL
                     RDVAHDQGLPIPLRVWTDGKLRLTFPTSSSVPKLNDQHAFVPCAGTHLRVGDVIEFGI
                     CHPCTTLDKHSVIYGLDGEARVASVFPTFFG"
     gene            3368..5437
                     /locus_tag="VHV99_12060"
     CDS             3368..5437
                     /locus_tag="VHV99_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX3636206.1"
                     /translation="MQSSYNFWLVAISFVVAALASYTALDLTGRIFLLASARRRHAWR
                     LGGAAALGVGIWSMHFIAMLAFSLPIPLGYDFATTACSLGLAIGASYLALVVTTHARL
                     TPLRLVAGGVLMGFGIAAMHYTGMAALQMKPGIHYQPVWLAGSLAIAIGASTAALWLA
                     RALSNGDERHVIRKRFIAALVMGVAISGMHYAGMAAAEFPSGAVCGAARGVNAAWLAT
                     SVILFTFAILIVTLLLSRFDARTSVLVGAVSKLNRQVVRLATLDTLTGLPNRSTLTER
                     IERAILDARRQRSLFAILFMDLDGFKTINDSLGHSAGDEVLSAFAQRLQLCVRASDTV
                     ARLGGDEFVVLSENLASREAAGTMAEGVLDRMRQGVWTDSQPLQVMPSIGIALYPHDG
                     DTVDTLLKHADVAMYEAKRGGRSAYRFFEQSMNEAATRTLQIQSALHEALTAGHFSLH
                     FQPKFQGNGDALAGAEALIRLHHPQLGMLAPLEFIPIAERSGQIVQIGYWVLRETCRQ
                     IRRWIEQGLPSIKVAINLSPRQLSQPGLVATMLEIVKGAGVRCEQIMFEITETVAMQD
                     APKTIEMIREFQASGFEIAIDDFGTGYSSLAYLQRFRVKQLKIDRFFTHGLDAHGPEG
                     SAIVSAIIALAHSLEMDVVAEGVETESQRDMLKSMRCDEMQGFLLGKPLTADDFGELL
                     RDRIVAA"
     gene            5551..5748
                     /locus_tag="VHV99_12065"
     CDS             5551..5748
                     /locus_tag="VHV99_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636207.1"
                     /translation="MPLVRLLLDRANMTLLYGHPRTTSALYAVVFAGDLLLTLGLIVL
                     ALHHLIVERDMLATLDRRLAT"
BASE COUNT         1153 a         1843 c         1693 g         1192 t
ORIGIN      
        1 cagcatggtc tgactcgccg cattcaggtt cagcgggaca acccgtaagc ccgtggtgct
       61 gctggctatc tgatagagga taggattgtc cgtgctatag ccgtcgatca tgcccgtctg
      121 cagtgcggtt gtcccttgcg catgatcagg gaaggccaac gttcgaaccg cgcctttgat
      181 cttgccctgc gtcaccgcat cctgatacat gatggactcg aggccgcctt gcggaatccc
      241 gactcgcctg ccggcgaggt cggccacact tttgatcggg ctgtccgcgc gtaccaggag
      301 accggtcgcc gttacgaaat gcgggatgct gaaatcgacg actttcgctc ggctgagtgt
      361 catcactgtt gcaccggctt cgatgtctat cgtgccgttc tgcagcagca cgattcgggt
      421 cgcgctcgtc agcggcacgt aattgacctt gatattgccg cccaactcgt ggcttatcgc
      481 ctggtaaatc gcattagcga tctcggtgga aaacccgaca tactgaccgc tcgtgttctt
      541 gaaactatag ggaggagcgg cttctcgcaa gccgatgtta aagacaccgg tccgcctgat
      601 tttgtcgagc gtgtcttcgg tggcgtcagc ggcatgcgcg ggcgcacaga ctgccagaca
      661 tgcacccaga ccgagcgtcc ccgacgacgc aaggaaagac cgtctgccgg cctgcttcgc
      721 caaacccaga ttcattttcg ccacgattgt cagctcctca tttgatgtag ccataccaac
      781 gcttcgccac gtcgcagtcc gatcgattca ctgacgacga cgccggcgtg acttgacgcc
      841 cactaatgcg agagaatctt ttcgatgaac cttgccgcac gctcgctttt cggctgacgg
      901 aagaacgtgt ccgccggcgt gtcttcgata atcacgccgc tttccatgaa cacgatgcgg
      961 tcggcaacgc gccgcgcaaa tcccatttcg tgcgtgacga caatcattgt catgttggtt
     1021 ttcgcgaggt tggccatcac ttccaggact tcgttgatca tttctggatc gagtgcggac
     1081 gtcggctcgt cgaacagcac cgcgataggg tctccagcca aagcccgcgc gatcgccacg
     1141 cgctgttgct gcccgccgga caagttgccg ggccagcgtt cggcgaacgc tgccatgcca
     1201 acccgctcaa gcagctctcg cgcgtagtgc tcggcatcgc ttctgtcttt tccaagcgca
     1261 acgacgggag caagcgtcag attgtcgatc acctttttat gcggatacag ctcgaagctt
     1321 tgaaacacca tcgaaatctt tctgcgcagc ttcaacaaat ctttctgcct tggcgtgacc
     1381 ttgcagccat cgacggtaat gacgccttgc tggatttcct cgagtccgtt gacacaccga
     1441 atcagcgtgc tctttccgga tccggacggt ccgcacacca ccaccacctc gcctttttcc
     1501 acattcagcg tacagtcgct gaggaccttg tgctgcccgt accatttgct gacagattga
     1561 agtgaaatca tcagatccag actcccagat aatttggcgc gcggcaggaa agcagggccc
     1621 cttcagccga agaacgtcgg gaatacgctt gcgaccctgg cctcaccgtc gagcccgtag
     1681 atcacgctgt gcttgtccag cgtcgtgcag ggatgacaaa tgccgaattc gatcacgtcg
     1741 ccaacgcgaa gatgggtacc ggcgcaagga acgaatgcat gctgatcgtt gagtttcggt
     1801 acgcttgacg acgtcgggaa tgtcaatcga agcttgccgt cagtccatac tcgcaatgga
     1861 atcggcagcc cctgatcgtg cgccacatca cgcaagccca gcccgcagat cgcgacgtcg
     1921 tcgtgaacgg acagcacttc cgcccagatg cgcagggtcg gcttgaatgc gtgggcaatg
     1981 acgtccgtca ccgcgtctcc cagcaaaccc cgttgcgcca tcgcctgcag gcgagctgaa
     2041 atcggcccgt ggtcgcagaa aaatacggcg ccgcaccgca agacaagtac gcaatcacga
     2101 tccgccgcga tgatcggcag agaatgggta atcacatggt cgaacaggct ggagccgcca
     2161 gcggtcaaaa tcaggcgttt gttcggcgct actgatttcc gcacggccgt cagagcggct
     2221 tcgactcgct ggaagagcaa gtccagattg atcttcagtt gggctgcatc gggggtgttc
     2281 gcggtgcctt cataagttgc gacgccggag aaactgagcc gctcattgtc acaacgctcc
     2341 accgcgcgga cgatgccatc gacttgcgcc tgggtttgca ccccccctct accgcaaccg
     2401 acttccagca gcaaaccaag cgccggaaga ttcgcgtctt ggcgccatac ggaatccaac
     2461 gcctcgacga agtcttccga gtcgacaaac acatggactt ccgcgttcgg gaatcgagaa
     2521 agaagatgcg caaggcgttt gattgcgttg cggccaccaa tttcattcgc aatcactatt
     2581 ctggtcaggc cgctatcgag catgatcgca gcctgcctga tgtccgcgac cgatatcgcc
     2641 cacgcgccgt tttcgaccag atcggctgcc agcgccgggg acatcggggt cttcgcatgc
     2701 ggcgcaatcg atatcccaaa gtgctggcaa atttttatca tcgcggaccg gttgcccgag
     2761 tacgcatcga gatcgagtga aagcagcggc agactcatct ttccgtcgaa cgggtgccaa
     2821 tcaaactggg caacctcatc agaccgcact cccgattgac ccggcggcac gccgcgcaaa
     2881 ccgtcgcgga gcatgaacgc tgcgccgcca accccattgc tccccaacat atcgttgtcc
     2941 ccgcagcatg ttgtcataac atatcctagc tagagatatt atctcaagtc aaccattcaa
     3001 aattaaataa gccataccgc atgaaggcga catacattgg gtgccgggac gcttgtgatt
     3061 cgaagcctgt gacgggaaaa gacagcgagc cagtcgagat gatggtcttg agctcctgaa
     3121 gtgtcgacat ggtcggtagc cgaatccagt ggtcacaatg atcatcgcga caggtgaagc
     3181 gtcagtggta gcaccacttg ccggttaatg tcatacctat tctagattga gatatgatca
     3241 ctggtcaact gcggacacac agccggtcga gccgatgtgt gaccgacata tcgcaaatca
     3301 attaaataaa cgccgccaac tgtcgataac aagatcagcc gcatcccctc tcgcggttgg
     3361 acttaacatg cagagctcat acaatttttg gctggtcgca atctcgtttg tcgtcgcggc
     3421 gctggcgtcg tacacggcgc tcgatttgac cggccgcatc ttcctgctgg cctcggcacg
     3481 gcggcggcac gcgtggcggc tgggcggcgc ggcggcgctc ggggtcggca tctggtcgat
     3541 gcacttcatc gcgatgctcg ccttctccct gccgattccg ctcggctacg atttcgcaac
     3601 gaccgcttgt tcccttggac tcgcaatcgg cgcctcgtat ctggcgctgg tcgtgacgac
     3661 tcacgcgcgc ctcacgccgc ttcggctcgt ggcgggcggc gtgctgatgg gtttcggcat
     3721 cgcagccatg cactacaccg gcatggctgc gctgcaaatg aagccgggca tccactatca
     3781 gccggtctgg ctggcgggct cgctggcgat cgcgataggc gcgtcgaccg ccgcgctgtg
     3841 gctggcgcgc gcactcagta acggcgacga acgccacgtg atacgcaaac gtttcatcgc
     3901 ggcgctggtg atgggcgttg cgatcagcgg catgcattac gccggcatgg cggccgccga
     3961 gtttccgtcc ggcgccgtgt gcggcgcggc gagaggcgtg aatgcagcgt ggctcgccac
     4021 ctcggtgatc ctgtttacct tcgcgattct gatcgtcacg ctgctgctgt cgcgtttcga
     4081 tgcgcgcacc agtgttctgg tcggggcggt gtcgaaattg aatcgccagg tcgtgcggct
     4141 cgccacactc gatacgctga cgggcctgcc caatcgcagc acgctcaccg aacgcattga
     4201 acgcgcgatc cttgacgcgc gccggcagcg ctcgctgttc gcgattctgt tcatggatct
     4261 cgacggcttc aagaccatca atgattcact cggccactcg gccggcgacg aagtgctgtc
     4321 cgcattcgcc cagcgccttc agctatgcgt gcgcgcgagc gacaccgtcg cgcgtctggg
     4381 cggcgacgaa ttcgtcgtgt tgtcggagaa cctggcttca cgcgaagccg ccggcacgat
     4441 ggccgaaggc gtgctcgacc ggatgcgcca gggcgtatgg accgattcgc aaccgctgca
     4501 ggtgatgccg agcatcggca tcgcgctcta tccgcacgac ggcgataccg tcgacacgct
     4561 gctcaaacac gccgacgtgg ccatgtacga agccaagcgc ggcggccgca gcgcctatcg
     4621 cttcttcgaa caaagcatga acgaagccgc gacgcgcacg ctgcaaattc aaagtgcgct
     4681 gcacgaagcg ctcaccgccg gccatttctc gctgcatttc cagccgaagt tccagggcaa
     4741 tggcgacgcg ctggcgggcg ccgaagcgct gatccgcctg catcatccgc aactcggcat
     4801 gctggcgccg ctcgagttca ttccgattgc cgagcgctca ggccagatcg tgcagatcgg
     4861 ctactgggtg ttgcgcgaaa cttgccggca gattcgccgc tggatcgagc aaggcctccc
     4921 gtcgattaaa gtggcgatca atctgtcgcc gcgccagttg tcgcaacccg gcctcgtcgc
     4981 gacgatgctc gagatcgtca aaggggcagg cgtgcgatgc gagcagatca tgttcgagat
     5041 caccgagacc gtcgcgatgc aggacgcgcc gaaaaccatc gaaatgatcc gcgaattcca
     5101 ggcaagcggc tttgaaatcg ccatcgacga tttcggcacc ggctattcca gcctcgccta
     5161 tctgcaacgc tttcgcgtca agcagttgaa gatcgaccgc ttcttcaccc acgggctcga
     5221 cgcacacgga ccggaaggca gcgcgatcgt ctctgcgatc attgcgctcg cgcactcgct
     5281 ggaaatggac gtagtggccg aaggcgtcga aaccgagtcg cagcgcgaca tgctcaagtc
     5341 gatgaggtgc gacgagatgc aaggctttct gctcggcaag ccactcaccg ccgacgattt
     5401 cggcgagttg ctccgtgaca gaatagtggc cgcatgagcc tgcggaaccg tttgaaaccc
     5461 gtatggctgc gcgcgtcagt agcggggcgc gtcatgaagg acacgcgctc tggcggcggt
     5521 gggtagcggt atcgcgcgct caatgccgtc gtgccgctcg tgcgcctgct gctcgatcgc
     5581 gccaacatga cgctgctgta tggccacccg cgcaccacct cggcgcttta cgcagtggta
     5641 tttgctggtg atctgctgct gaccctcggc ctgatcgtgc tggccttgca ccatctgatc
     5701 gtcgaacgcg acatgctcgc cacgctcgac cgccgcctag ccacttaact ggccttgcgc
     5761 tccccatgtg ccgtatcggc caccgacacg agaatcgacg tcacagcctg cgcggcttca
     5821 cgcttggtga gcttgccgtc gaccgaggcg ccgcaatcca tccgcaactg ctggtagtag
     5881 a
//
LOCUS       DATASH010000435         4546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2045999,
            whole genome shotgun sequence.
ACCESSION   DATASH010000435 DATASH010000000
VERSION     DATASH010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4546
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2045999"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..1103
                     /locus_tag="VHV99_12070"
     CDS             78..1103
                     /locus_tag="VHV99_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX3636208.1"
                     /translation="MKELGKISQKSVASDQVSMIEIDDSAAGQRIDNFLLRVCKGVPK
                     SHIYRILRSGEVRVNKGRIDAQYRLELGDLVRVPPIRVAQPNEAIAQAPVPSADFKII
                     FEDEHMLVIDKPAGVAVHGGSGVAFGVIEQMREARPQAKFLELVHRLDRETSGILMLA
                     KKRAALVNLHEQIRENKMDKRYYACVHGEWASDWGRRRAVKEPLHKYLTADGERRVRV
                     QADGLASHTVFNLVDKWAEYALLEAELKTGRTHQIRVHLQHLELPIVGDAKYGDFALN
                     KALARANAKPGLKRMFLHAYRLKLTHPATGAPVQFEAPLPAECRSFVAQLNELRNGSN
                     PETTPHG"
     gene            1096..1755
                     /locus_tag="VHV99_12075"
     CDS             1096..1755
                     /locus_tag="VHV99_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX3636209.1"
                     /translation="MAREQFDLIVFDWDGTLMDSTAHITRSIQSACRDLGLPVPADEA
                     ASYVIGLGLRDALQIAAPTLDPVDYPRLAERYRFHYLVKDQTTELFVGVREMLQDLRD
                     QGYLLAVATGKSRVGLNRALDQSRLTSLFDGTRCADETFSKPHPAMLHELTRELGQDP
                     VRTVMVGDTTHDLQMAINAGVAGIGVTYGAHPAGSLTALSPKFVASSVGALSGWLREN
                     A"
     gene            1752..2156
                     /locus_tag="VHV99_12080"
     CDS             1752..2156
                     /locus_tag="VHV99_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX3636210.1"
                     /translation="MSGGTSEAATEAVRICASDELVDGGAGVRRAATLGGGDVVVFFV
                     RYDGHAYGYLNRCAHVPMELDWAEGQFFESSGLYLMCATHGAIYAPDTGKCVGGPCRG
                     GRLRPVQVDERDTPEGRAVFWLPDGELRPATP"
     gene            2191..3195
                     /locus_tag="VHV99_12085"
     CDS             2191..3195
                     /locus_tag="VHV99_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEX3636211.1"
                     /translation="MSDNLTPEPKEPSLTGRARTPADEPGWERAALERIALAAITEQR
                     AARRWKIFFRFVFLIVLLLAIWGALDFSGDKVSATGRHTAMVTIDGEISADTNANAED
                     INTALESAFDDSGTAGVILRCNSPGGSPVQAGIIYNEIHRLRAKYPSIPLYVVVGDMC
                     ASGGYYAAAAADKIYVDKASIVGSIGVLMDSFGFTGLMDKLGIQRRLHTSGENKGFFD
                     PFSPETPKMDEHAQDMLDQIHAQFIDAVRQGRGKRLHETPDMFSGLFWTGQKSVELGL
                     ADGFGDADYVARELFKAPDIVDYTVKESITDRVARKFGAAVGSGAVHAMALGGKMSLR
                     "
     gene            complement(3360..4079)
                     /locus_tag="VHV99_12090"
     CDS             complement(3360..4079)
                     /locus_tag="VHV99_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX3636212.1"
                     /translation="MSGTLYLIPNTLGEGDADALDAVLPAPVRARAASLHYYIGENAK
                     TTRAFLKKVGTEKPIQEIEIRELNVNTPAGEIDKLLAPLLAGTDAGLVSEAGCPAVAD
                     PGALLVRRAHERGVKVVPFVGPSSILLALMASGLNGQSFAFHGYLPVDAIERAKRLRE
                     LEQQSRKGKQTQIFIETPYRNRALLDTLLATCAPSTLVCVAVDLTLETETIASRTVAD
                     WKKKPAIDLHKRPAIFLILAV"
     gene            complement(4076..>4546)
                     /locus_tag="VHV99_12095"
     CDS             complement(4076..>4546)
                     /locus_tag="VHV99_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEX3636213.1"
                     /translation="VTALRLAEAKARAVAARLGAGEAALVIGSDQVATYDGLQIGKPG
                     THDKALAQLQAMRGREVLFHSALCLFDTRSSTAQAVDIITRVQFRDLPDAALDAYLRA
                     ETPYDVAGSAKSEGLGIALLEAIHSDDPTALVGLPLIALSRMLLAVGYPLLGAA"
BASE COUNT          848 a         1347 c         1484 g          867 t
ORIGIN      
        1 gcttgcacct gcgcaatccg cccgaattcc ggacactgcg ccggccgccg cagactgctg
       61 ggcaaattat attcataatg aaagagttag gcaaaatatc ccagaaatcg gtcgcaagcg
      121 accaggtctc gatgatcgag atcgacgaca gtgcggccgg acagcgcatt gataatttcc
      181 tgttgcgcgt ctgtaaaggc gtgccgaaaa gccatattta ccgcatcctg cgcagcgggg
      241 aagtgcgggt aaacaagggc cggatagacg cgcagtaccg cctcgaattg ggcgatctgg
      301 tgcgtgtgcc gcccattcgc gttgctcaac ccaatgaagc gattgcccag gcaccggtgc
      361 cgagcgcgga tttcaaaatt atttttgaag acgagcacat gctcgtgatc gacaaaccag
      421 ctggcgtggc ggtccacggc ggcagcggcg tcgcgttcgg cgtcatcgaa cagatgcgcg
      481 aggcgcggcc gcaggcgaaa ttcctcgaat tggtgcatcg gctggatcgc gagacgtccg
      541 gcatcctgat gcttgccaag aaacgcgccg cgctggtcaa tctgcacgag cagattcgcg
      601 agaacaagat ggataagcgc tactatgcgt gcgtccatgg cgagtgggcg agcgactggg
      661 gtcgccgccg cgcggtcaag gagccgctcc acaagtattt gaccgcggac ggcgaacggc
      721 gtgtgcgtgt ccaggccgac gggctcgcgt cgcacacggt gttcaatctg gtcgacaaat
      781 gggcggagta cgcgctgctc gaagcggagc tgaagacggg ccgtacgcat cagattcgcg
      841 tccatttgca gcatctggaa ctgccgatcg tcggcgatgc aaaatacggc gacttcgcgc
      901 tgaacaaggc gctggcgcgc gccaatgcga agccgggcct gaaacgcatg ttcctgcacg
      961 cataccgtct taagctgacg cacccggcaa ccggtgcgcc cgtgcagttc gaggcgccgc
     1021 tgccggccga gtgccgcagt ttcgttgcgc agctcaatga attacgaaat gggtcaaacc
     1081 cggagacgac accgcatggc tagagagcaa tttgatctga tcgtcttcga ctgggacggg
     1141 acgctgatgg attcgaccgc gcacatcacg cgcagcatcc agtcggcgtg ccgcgatctc
     1201 ggcttgccgg ttcccgcaga cgaggccgcc agctacgtga tcggcctcgg cttgcgtgac
     1261 gcgctgcaga tcgccgcgcc cacgctcgat ccggtcgatt acccgcgtct ggcggagcgc
     1321 taccgcttcc actatctagt aaaggaccag actaccgagt tgttcgtcgg cgtgcgcgag
     1381 atgttgcagg atttgcgcga tcagggctat ctgctggcgg tggcgaccgg caagagccgc
     1441 gtcgggctga accgcgcgct cgatcaatcg cgtctgacga gcctgttcga cggcacgcgc
     1501 tgcgccgacg aaaccttctc caaaccccat ccggccatgc tgcatgagct cacgcgtgaa
     1561 ctggggcagg atccggtgcg cactgtgatg gtcggcgata cgacgcacga tctgcagatg
     1621 gcgatcaacg cgggcgtggc gggcatcggc gtgacatacg gggcgcatcc ggccggctcg
     1681 ctgaccgcgt tgtcgccgaa attcgtcgcg tcgagcgtgg gcgcgctgtc cggctggctg
     1741 cgagagaacg catgagcggc ggcacgagcg aagcggccac ggaagcggtt cgcatttgcg
     1801 cgtctgacga actggtcgac ggaggtgcgg gcgtacggcg cgcggccacg ctgggcggtg
     1861 gcgacgtagt ggtatttttt gttcgctatg atggccatgc ctacggctat ctgaatcgct
     1921 gcgcccacgt gcccatggaa ctcgactggg ccgaagggca gttcttcgaa tcctccggtt
     1981 tatacttgat gtgcgccaca catggcgcga tttacgcgcc tgatacgggc aaatgcgtgg
     2041 gtggcccgtg ccgcggcggg cggttgcgtc cggtgcaggt tgatgagcgg gatacacctg
     2101 aaggccgcgc cgtattctgg ctgccggatg gcgagctgcg ccccgccacg ccctgatttt
     2161 ttctcctctt cgacctttcc actgcaccgc atgtccgaca atttgacgcc tgaaccgaaa
     2221 gaaccgtccc tgacaggccg cgcccgcacg cctgccgatg agccgggctg ggagcgcgcc
     2281 gcgctcgagc gcatcgcgct tgcggcaatc actgagcagc gggcggcacg ccgctggaag
     2341 atctttttcc gctttgtgtt cctgatcgtc ttgctgctgg cgatatgggg tgccctcgat
     2401 ttctctggcg acaaggtttc cgctaccggc cggcacacgg caatggtgac aattgacggg
     2461 gaaatctccg ccgatacgaa cgcgaacgca gaagatatca acacagcgct ggaaagtgca
     2521 ttcgacgatt cgggcacggc aggcgtgatt ctgcgctgca atagtcccgg tggcagcccg
     2581 gtgcaggcgg gcatcatcta caacgagatt caccggctgc gcgccaagta tccgtcgatc
     2641 ccgttgtacg ttgtggtcgg cgatatgtgc gcctcaggcg gctattacgc agccgcggcg
     2701 gccgacaaga tttacgtcga caaggcgagc atcgtcggct cgattggcgt gctgatggat
     2761 agcttcggct ttacgggtct gatggataag ctggggattc agcgtcgtct gcacacttca
     2821 ggtgagaaca aaggcttttt cgacccgttc tcgccggaaa cgccgaagat ggacgaacat
     2881 gcgcaagata tgctcgatca gatccacgct cagttcatcg acgccgtccg tcagggccgt
     2941 ggcaagcggc ttcacgaaac gccggatatg ttttcgggcc tcttctggac gggtcagaag
     3001 agtgtcgagc tggggctcgc agacggtttt ggcgacgcgg attacgtcgc gcgtgagctg
     3061 ttcaaggccc cggatatcgt cgactacacg gttaaagaga gcattacgga ccgcgtagcg
     3121 cgcaaattcg gcgcggcggt tggcagtggc gccgttcacg cgatggcgct tggcgggaag
     3181 atgagcttgc ggtgattatt tgcctgtgag aagccccgcg cggccaagtg atggccgcgc
     3241 ggggtttttt tgtttcttcg cattgaacgg cgatcggcga gttgagcgat gggcgccctc
     3301 agtcgatctg gaacgccggc acgaccctgt tcgcgggcac ggttaagttg cagcaacgat
     3361 cagacggcga ggattaaaaa aattgcgggc cgcttgtgca gatcgatagc cggtttcttt
     3421 ttccagtcgg ccacagtgcg gctggcgatg gtttccgtct ccagcgtcag atcgactgca
     3481 acgcaaacga gagtcgatgg cgcgcaggtc gccagcaacg tgtcgagcaa ggcgcgattg
     3541 cgatacggcg tctcgataaa gatttgtgtc tgcttgcctt tgcgcgactg ttgctccagt
     3601 tcgcgcagac gcttggcccg ctcgatggcg tcgaccggca ggtaaccgtg gaacgcaaaa
     3661 ctctggccgt tcaggccgga agccatcagc gccagcaaaa tcgagctcgg tccaacaaac
     3721 ggcacgacct tcacaccgcg ctcgtgcgcg cggcgcacca ggagggcgcc cgggtcggcg
     3781 actgccgggc agccggcttc ggacaccaaa cccgcatccg tgccggccag caacggcgcg
     3841 agcagcttgt cgatttcgcc cgccggcgtg ttgacgttca attcgcgaat ctcgatttcc
     3901 tgaatcggct tttcagtgcc gaccttcttc agaaaagcac gggtggtttt cgcgttttcg
     3961 ccgatgtaat agtgcagcga tgccgcgcgc gcccggaccg gcgcgggcag gacggcatcg
     4021 agcgcgtcgg cgtcgccttc gccgagcgtg ttcgggatca gatataaggt gccgctcatg
     4081 ctgcccccag gagtggatag cccactgcga gcagcatgcg cgacagtgcg atcaatggca
     4141 ggccgaccag cgcggtcggg tcgtccgagt gaatggcttc gagcaacgcg atgccgagac
     4201 cttcggattt ggcgctgccc gcgacgtcgt agggtgtttc agcgcgcaga tacgcgtcta
     4261 gcgccgcgtc cggcaggtcg cggaattgca cgcgcgtgat gatgtcgacg gcttgcgccg
     4321 tgctggagcg ggtatcgaac agacacaacg cactatgaaa cagcacttcg cggccgcgca
     4381 ttgcttgcaa ctgggcgagc gccttgtcat gtgtaccggg cttgccgatc tgcagaccgt
     4441 cgtaagtagc gacctgatcc gagccgatca cgagtgcggc ttcaccggcg ccgagcctgg
     4501 ctgccaccgc gcgggctttt gcctcggcga gccgcagggc cgtcac
//
LOCUS       DATASH010000436         1746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6199024,
            whole genome shotgun sequence.
ACCESSION   DATASH010000436 DATASH010000000
VERSION     DATASH010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6199024"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1016
                     /locus_tag="VHV99_12100"
     CDS             <1..1016
                     /locus_tag="VHV99_12100"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX3636214.1"
                     /translation="ESRYRVSQEALRPYFPLPKVLDGLFVLTQRLYGISVRERTDVKV
                     WHPSVRFYDLLDKDNRVVAGFFLDPYSRAEKRSGAWMDECVVAKALPSGTALPVAQLV
                     CNFTAPVGPQPSQLTHDEVITLFHEFGHRLHHMLTRVAYPSIAGINGVAWDAVELPSQ
                     FMENFVWRAEVLPLISAHIDSGEPLPVDMLHRLLGTRTFNAALDTLRQIELATFDFEL
                     HANFDPQVGANVAATLAAVRQRVAVVPAAAFNRMPASFAHIFAGGYAAGYYSYKWAEV
                     LAADAFEAFEEAGVFDADTAARFRESILSRGGSVDAMEAFVRFRGRQPDVRPLLKQTG
                     IAA"
     gene            1013..1606
                     /locus_tag="VHV99_12105"
     CDS             1013..1606
                     /locus_tag="VHV99_12105"
                     /inference="COORDINATES: protein motif:HMM:TIGR04211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HEX3636215.1"
                     /translation="MKRLAALLLALLSIKGVAATAYVSDELVLGVYAEENNQGQRLAT
                     LHSGTGVETLAQNGDYTQVRLSDGTTGWVKSAFLTAKEPAVVRVKRLEEELDRSRATT
                     PALAEAAARSEVEQLKLELAAKQSELDAVRGGSPPAMSVSPPTTGALAALDAAAAPRW
                     PVITAIAAALGLACGFWLGFATLARRVRDKFGGIKVY"
BASE COUNT          315 a          530 c          567 g          334 t
ORIGIN      
        1 aagagagccg ctatcgggtc tcgcaggagg cgttgcgccc ttacttcccg ctgccgaagg
       61 tcctcgatgg cctgttcgtt ctgacgcagc gcctgtacgg gatcagcgtt cgcgagcgca
      121 ctgatgtcaa ggtgtggcat ccgagcgtgc gcttctacga tttgctcgac aaggacaacc
      181 gcgtcgtagc gggttttttt ctcgatccct attcgcgtgc cgaaaagcgc agcggcgctt
      241 ggatggacga atgcgtggtc gcgaaagcgc tgccgtcagg cacggcgctt cccgtcgcgc
      301 agctggtgtg caatttcacc gctcccgtag gtccgcaacc ctcgcagctg acgcacgatg
      361 aagtgatcac gctgtttcat gagttcggcc accggctgca tcacatgctg acgcgcgtgg
      421 catatcccag catcgccggt atcaacgggg tcgcctggga tgcggtcgag ctgccgagtc
      481 aattcatgga aaacttcgtc tggcgcgcgg aagtgctgcc gctgatttca gcacacatcg
      541 acagcggcga accgctgccg gtcgacatgc tgcaccggct gctgggtacg cgcaccttca
      601 acgccgcgct cgatacattg cggcagatcg aactcgctac cttcgacttt gagttgcacg
      661 ccaatttcga tccgcaggtg ggcgccaatg tcgcagcgac tctggcggca gtgcgccagc
      721 gagtggcggt cgtgcccgcg gcagccttca atcggatgcc ggcgagcttc gcgcacatct
      781 tcgcgggcgg ctacgccgca ggctattaca gttacaagtg ggccgaggta ctggccgcgg
      841 atgccttcga ggctttcgag gaagccgggg ttttcgacgc cgacacggcg gcgcgctttc
      901 gtgagtccat cctcagcaga ggcggcagcg tggatgcgat ggaggcgttc gtccgctttc
      961 gtgggcgcca accggacgtg cgtcccctgc tcaaacaaac gggaatcgcc gcgtgaagcg
     1021 gctcgccgcg ctgctgttgg cgctgctgtc gatcaaaggt gtggccgcta ccgcttacgt
     1081 gagcgacgag ctggtgctcg gcgtgtacgc cgaagaaaac aatcagggac aacgactggc
     1141 gacgctgcat tcgggcaccg gcgtggaaac gctggcgcaa aacggcgatt acacccaggt
     1201 tagattgagc gacggcacga caggctgggt caagtcggct tttttgactg ccaaggaacc
     1261 tgccgtcgtt cgcgtcaaac ggctcgagga ggagttggat cgcagccgag ccaccacgcc
     1321 ggcgctggcc gaggcggccg cgcgcagcga ggtcgaacaa ttgaagctgg aacttgccgc
     1381 aaagcaaagc gagttggatg ccgtgcgggg cggatctccg ccggcgatga gcgtctcgcc
     1441 gccgaccacc ggagcgctgg cggcgcttga tgcggcggcg gcgccccgct ggcccgtgat
     1501 caccgctatc gcggccgccc tcggcctggc ctgcggtttt tggctggggt ttgccaccct
     1561 ggcgcggcgt gtacgcgaca aattcggggg tatcaaagtt tactaaatgc tgaattttcg
     1621 ccacccagga ttgacttaat attgtgagct acgtccagaa ggagaagtag tcacgtagcg
     1681 ccaccggccc tgagagccta aactcccctt gatgtacgac ctcagacgta acgattacga
     1741 cgtcgc
//
LOCUS       DATASH010000437         4371 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4222541,
            whole genome shotgun sequence.
ACCESSION   DATASH010000437 DATASH010000000
VERSION     DATASH010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4371
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4222541"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1250
                     /locus_tag="VHV99_12110"
     CDS             <1..1250
                     /locus_tag="VHV99_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese homology domain-containing protein"
                     /protein_id="HEX3636216.1"
                     /translation="KSFGEFVEAKRHTPALSAEEVDALLKSGEDVVVLDARRYDEYQT
                     MNIPGSISVPGAELVLRARALAPDPNTRVIVNCAGRTRSIIGTQSLVNAGIPNPVAAL
                     RNGTIGWLLAGQSLAHGSARQFGTDDGRADAASRESARAVADRAGVKRTTLAEAQRWT
                     DDANRTTYRFDVRTPAEYEHAHAAGFQGAPGGQLVQETEMFAPVRGARVVLFDDDGVR
                     ANMTASWLAQMNIDVYVIDDATSADLTEHGPWRAAKPLPAATPTITANELAALLDEPA
                     SQTIVLDFTASANHVKGHIPGAWWTVRSRLALDLPALPDAKQYVVTCGSSALAAFAGP
                     EVAALTSKPVLLLEGGTAAWTAAGLPLAKGETHLASPRIDRYRRPYEGTDAPHEAMNA
                     YLEWEYGLVAQLERDGTHGFFVI"
     gene            1377..2312
                     /locus_tag="VHV99_12115"
     CDS             1377..2312
                     /locus_tag="VHV99_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3636217.1"
                     /translation="MKRRSLLLIAALTAFTALRPLASVAEPVLKVGDQQLQVRGILEA
                     SGQLKEVPYKIEWFNFPAAQPLGEALNAGAIDIGGLGDAPLIFAYAAGARVRAVAATR
                     SQPVDLAILVPAGSSIKSAADLKGKRIATTRGSIGHFLAIATLERAGIKLSDVTLEFM
                     QPADAKSALESGNVDAWSTWDPYVALGELRDNNRSIANGVGLSSGLSFQAATDESIKT
                     KRAQIDDFVRRVAAGQQWALSHPDEVAAIQSRVTHLPADVLKVVYQRAQLKPITIDSK
                     VIAEQQRTADLYVRAGVIKTTLDVTPSFDARFTAH"
     gene            2337..3218
                     /locus_tag="VHV99_12120"
     CDS             2337..3218
                     /locus_tag="VHV99_12120"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX3636218.1"
                     /translation="MTVRHPPTALQDPIALDALADLDSTAADQWLAALTQDFAASAGA
                     LDREAIFPHDNLARLRSAGLLALTVPQALGGRQATLPQALKVVRAVARGEPSTALILV
                     MQCLYHLRLQANPNWPQALKEQVARDAVENGALINSLRVEPELGSPSRGGLPGTTGSL
                     TDNGDAWILNGSKIYSTGSPGLTWFAVWGRTDESSPRVGTFLVHRDTIGIRLGEPWNH
                     LGMRATGSHEVIFENVRVPAAHAVDFNPPEIAASGMDPLTTLWMNVLLSSIYDALARC
                     ARDWFASWAADRKPANL"
     gene            3273..3569
                     /locus_tag="VHV99_12125"
     CDS             3273..3569
                     /locus_tag="VHV99_12125"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX3636219.1"
                     /translation="MLLNNRVLLDAGSHGSIALHEAPAIKYLVTQNAITAVELAVEAS
                     GNPGLSRNNPLERHHRDVLCSRIHTPQNDALLGAFGRTGFATLQARQSLKGAPL"
     gene            3566..>4371
                     /locus_tag="VHV99_12130"
     CDS             3566..>4371
                     /locus_tag="VHV99_12130"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX3636220.1"
                     /translation="MSVEFIGYVSHQESSEIHLPQGPAVNPPYIAAVAQAHEYGGFDR
                     VLIAHSSASPDGFQIASFVAQQTRHLGILLAHRPGFVAPTLAARQLATLDHFSAGRTA
                     VHIISGGDDTEQQRDGDFLEHDDRYKRTDEYLDVVKRSWTSETPFDHEGTFYRVKGHR
                     SLVRPVQKPHLPVYFGGSSEAAIQVAAKHADVFALWGESLAQVAETIARVRAAAKPYG
                     RENAIRFSLSLRPIIASTEDAAWARAESILARATEVVKSNPNFTRRPTEP"
BASE COUNT          846 a         1411 c         1336 g          778 t
ORIGIN      
        1 gcaaatcgtt tggtgaattc gtggaagcga aacggcatac accggcgtta tcggcggaag
       61 aagtcgatgc gttgctgaaa agcggcgagg acgtggtcgt gctcgacgcg cgccgctacg
      121 acgaatacca gacgatgaac attcccggca gcatcagcgt gccgggcgcc gaactggtgt
      181 tgcgcgcccg cgcgcttgcg cccgatccga atacacgcgt gatcgtgaat tgcgcggggc
      241 gtacgcgcag catcatcggc acgcagtcgc tggtgaacgc cgggattccg aacccggtgg
      301 cggcgttgcg caacggcacg attggatggc tgctcgcagg ccagtcgctc gcgcatggca
      361 gcgctcggca gtttggcacc gacgacggcc gtgccgacgc cgcttcgcgt gaatcggccc
      421 gggccgtagc cgatcgcgcg ggcgtgaaac gcacgacgct cgcagaagcg caacgctgga
      481 cagacgatgc aaaccgcacg acgtatcgct tcgatgtccg cacgccggcc gaatacgaac
      541 acgcgcatgc agcgggtttt caaggcgcgc cgggcggcca gctcgtgcag gaaacggaga
      601 tgttcgcgcc ggtccggggc gctcgcgtgg tcctcttcga cgacgacggc gtgcgcgcca
      661 acatgacggc gtcatggctc gcgcagatga acatcgacgt gtatgtgatc gacgacgcga
      721 cgagcgccga cctgaccgaa cacggcccgt ggcgcgccgc gaaaccgctg cccgcagcca
      781 cacccacgat caccgcgaac gaactcgccg ccctgcttga cgagcccgca agccagacca
      841 tcgtgctcga cttcacggcg agcgccaacc atgtgaaggg acacatcccc ggcgcatggt
      901 ggaccgtgcg ctcgcggctt gcgctcgacc tgcccgcgtt gccggacgcg aaacaatacg
      961 tggtcacgtg cggatcgagc gcgcttgccg cgttcgccgg gccggaagtg gcggcgctta
     1021 catcgaagcc cgtgctgttg ctcgaaggcg gcaccgctgc atggaccgcg gcaggcttgc
     1081 cgcttgccaa aggcgagacg catctggcgt cgccgcgcat cgaccggtat cggcgtccgt
     1141 atgaaggcac agacgccccg cacgaagcaa tgaacgcgta ccttgaatgg gagtacggtc
     1201 tcgtcgcgca actcgaacgt gacggcacgc atggcttctt cgtgatttga agcagcaggt
     1261 ctgacgcggc tcgatccgcg tcattccaat tccggcgatt agcaaagcag aacaatgcgt
     1321 ttgtctaatg ccgtggcgga ccgtatcgtg aatcctttct cctgcatgga cgattcatga
     1381 aacgacgcag tctcctcctg atcgccgcgc tgactgcatt caccgcgctg cgccctcttg
     1441 cttcggtagc cgaacctgtt ctgaaagtcg gcgaccagca actacaggta cgcgggattc
     1501 tcgaagcatc cggccagttg aaagaggtgc cgtacaagat cgagtggttc aactttcccg
     1561 ccgcgcaacc gctcggtgag gccttgaacg caggcgcaat cgatataggc ggtctgggcg
     1621 atgccccgct gatcttcgct tacgccgccg gtgcgcgcgt gcgagcggta gcggcaaccc
     1681 gctcgcaacc ggtcgacctc gcgattctcg tgccggccgg ctcctccata aaaagcgcgg
     1741 ctgatctgaa gggcaaacgc atcgcgacca cgcggggttc catcggccat tttctggcca
     1801 tcgcgacgct cgaacgcgcg ggcatcaagc tctctgacgt gacgcttgag ttcatgcaac
     1861 ctgccgatgc caagtcggcg ctggagtcgg gtaacgtcga tgcgtggtcc acatgggacc
     1921 cctatgtcgc gctcggtgaa ttgcgcgata acaatcgcag catcgcgaat ggcgtgggac
     1981 tttcatcggg tctgagcttt caggcagcta ccgatgaatc gatcaagacc aaacgtgcac
     2041 aaatagatga cttcgtgcgg cgcgtagcgg ccggtcaaca atgggcgttg tcgcatcctg
     2101 acgaagtcgc ggctatccag tcgcgcgtta cgcacctgcc cgccgatgtg ctgaaggtgg
     2161 tgtatcaacg ggcgcagttg aagccgatca cgattgacag caaggtgatc gccgagcagc
     2221 agcgcaccgc cgatctctac gttcgcgcgg gcgtgatcaa gacgacgctc gacgtgacgc
     2281 caagtttcga cgcccggttc acggcgcatt aacactcgtc aataacggac tttccgatga
     2341 ctgtacggca tccgcccaca gcgttgcagg acccgattgc gctcgacgcc ctcgccgatc
     2401 tcgacagcac cgccgccgat caatggctcg ccgcgctcac tcaggatttc gctgcgagcg
     2461 ccggcgcgct ggaccgtgaa gccatctttc cccacgacaa cctcgcgcgt ttgcgaagcg
     2521 ccggcttgct cgcgctgacg gtgccacaag cgcttggcgg ccgccaggct acgctgccgc
     2581 aagcactcaa agtagtgcgc gccgttgcac gcggtgagcc gtcgacggcg ctgatcctcg
     2641 tgatgcaatg tctttatcat ctgcgcctgc aggccaatcc aaactggccg caagcgctga
     2701 aggaacaagt cgctcgtgat gcagtggaaa acggcgcact catcaactcg ctgcgcgtag
     2761 agcccgagtt aggctcgccc tcgcgcggcg gtttgccagg cacgaccggc agcctgaccg
     2821 ataacggcga cgcgtggatt ctcaacggca gcaagatcta ttcgacgggc agtccgggcc
     2881 tgacgtggtt cgctgtttgg ggccgcacgg acgaaagctc gccgcgcgtg gggacattcc
     2941 tggtgcatag ggatacgatt ggcattcggc tcggcgagcc gtggaatcac ctcggcatgc
     3001 gtgcaactgg cagtcacgaa gtgatcttcg agaacgtgcg tgttcctgct gctcacgcag
     3061 tcgacttcaa tcctcccgag atcgccgcga gcggcatgga cccgctcacc acgctgtgga
     3121 tgaacgtgct gctttcatcg atctacgacg cgctcgcccg gtgtgctcgc gactggtttg
     3181 catcgtgggc cgccgatcgc aaacccgcca acctgtgagc accgctgtcg agcctgggta
     3241 gtttccagca gacgctcggc cgtatcgacg cattgctgct caacaaccgc gtgctgctgg
     3301 acgcgggctc acacggcagt attgcgctgc atgaagcgcc cgccatcaag tacctcgtga
     3361 cgcagaacgc cattaccgcc gttgaactcg cggtcgaggc cagcggcaat ccagggctga
     3421 gccgcaacaa cccacttgaa cgccatcatc gcgacgtgct gtgctcgcgc attcatacgc
     3481 cgcaaaacga cgcgctgctc ggcgcattcg ggcgcaccgg tttcgccacg cttcaagcca
     3541 gacaatctct taagggagca ccactatgag cgttgaattc atcggctacg tgagccatca
     3601 ggaatcaagc gaaatccact tgccgcaagg acccgcggtc aatccgccat acattgccgc
     3661 agtcgcacaa gcgcatgaat acggcggctt cgaccgcgtg ctgatcgcgc attcaagtgc
     3721 gtcgccggac ggcttccaga tcgcgtcgtt cgtcgcgcaa cagaccaggc atttgggcat
     3781 cctgcttgcg catcgcccgg gctttgtcgc gccgacgctc gcggcccgtc aactcgccac
     3841 gctcgatcac ttcagtgcag gccgcaccgc cgtccacatc atctccggcg gcgacgatac
     3901 cgagcaacaa cgcgatggcg acttcctcga acacgacgac cgctacaagc gcaccgatga
     3961 atatctcgat gtcgttaaac gctcatggac cagcgaaacg ccgtttgatc atgagggcac
     4021 gttctatcgg gtgaagggtc accggtcgct ggttcgtccc gtgcagaagc cgcatctgcc
     4081 ggtgtatttc ggcggttcgt cggaagcagc aatccaggtc gcggccaagc atgccgacgt
     4141 ttttgcgctc tggggcgagt cgctggcgca ggtcgccgag accatcgccc gggtgcgcgc
     4201 ggccgccaaa ccgtatggcc gtgagaacgc tattcgcttc agcctctcgc tgcgtccaat
     4261 catcgcttcg acggaagatg ccgcatgggc acgtgcggaa tcgatccttg cacgtgcgac
     4321 agaggtagtg aaaagcaatc ccaactttac gcgccgacca acagaaccca g
//
LOCUS       DATASH010000438         2501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3827011,
            whole genome shotgun sequence.
ACCESSION   DATASH010000438 DATASH010000000
VERSION     DATASH010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3827011"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..831)
                     /locus_tag="VHV99_12135"
     CDS             complement(25..831)
                     /locus_tag="VHV99_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX3636221.1"
                     /translation="MKIRVLSDLHLENDEPELIPHAHADLIVLAGDIHNHAAGPRWAA
                     QTFDGAVPVVYVPGNHEYYDGEFGALDTALYDAAAQVDNVHVLNNAALVDPQGRWRVL
                     GTTLWTDFALYGADPDTLAECIDASRRAMLDFRGLIQMNWPHDTQDSPRDFTPGDSLA
                     LHRQARAWLENELARPFDGKTIVVTHHAPHRLSLAERYAHDRVSAGFVNHLPELVHAP
                     VALWIHGHTHTPFDYTVNGTRVVCNPRGYFDRRTGQWENPQFTWDKVVEI"
     gene            complement(983..2401)
                     /locus_tag="VHV99_12140"
     CDS             complement(983..2401)
                     /locus_tag="VHV99_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX3636222.1"
                     /translation="MTQAAFLTACRDVIGAAQVLTDPHDTAPYLTDWRRRYTGAACAV
                     LCPATPDEVAALVKLAIEHRIALVPQGGNTGLAGGATPDASGAQAVISLRRLNRVRDI
                     DPHNNTITVEAGVILADVQRHAGQAGRLFPLSLAAEGSCTIGGNLATNAGGTGVLRYG
                     NTRELCLGLEVVTPQGELWDGLRGLRKDNTGYDLRDLFIGAEGTLGIITAAVLKLHPQ
                     PAARVTALAALASPHAALDFLSLTQRVAGPLLTGFELMSDFCLRLVGRHFEQMRYPFA
                     EPHAQVVLLELSDSESEEHARGLFERLMETALEEGLVEDAVVAENLTQSRAFWNLREH
                     IPLAQAEEGLNIKHDIAVPISRIGHFIEETDAAIAQAVPGVRMVTFGHLGDGNLHYNV
                     QAPEGVDAKAFLKTWQSPINQLVYDSVHRHRGSISAEHGLGQLKIDEEMHYKQDVEVQ
                     LMRAVKRALDPLNLMNPGKVLR"
BASE COUNT          429 a          773 c          834 g          465 t
ORIGIN      
        1 acccagatcg gcccgaagcc gatgctatat ctccacgacc ttgtcccacg tgaactgcgg
       61 attctcccac tgcccggtgc gccggtcgaa atagccgcgt ggattgcaga ccacgcgcgt
      121 gccgttcacg gtgtaatcga acggggtatg cgtatgccca tgaatccaca gcgcgaccgg
      181 cgcgtgcacc agttccggca aatgattgac gaaaccggcc gacacgcgat cgtgcgcgta
      241 gcgctcggcc agactcagcc gatgtggcgc gtgatgcgtg acgacgatcg tcttgccgtc
      301 gaacggtctg gcgagctcgt tttcgagcca ggcgcgcgcc tggcggtgta gcgcgagaga
      361 atcgccaggg gtgaagtcgc gtggcgagtc ctgggtgtcg tgcggccagt tcatctggat
      421 caggccgcgg aagtcgagca tcgcccggcg cgaagcgtcg atgcactcgg cgagcgtgtc
      481 aggatccgcg ccgtataacg cgaagtcggt ccacaaggtg gtgccgagca cgcgccagcg
      541 gccttgcggg tcgaccagcg cggcattgtt cagcacatgc acgttgtcga cttgcgcggc
      601 tgcgtcgtag agggccgtgt cgagcgcgcc gaattcgccg tcgtagtatt cgtgattgcc
      661 gggcacgtag acgaccggca cggcgccgtc gaaggtttgc gcggcccagc gcgggccggc
      721 cgcatgattg tggatgtcgc cggccagcac gatcagatcc gcgtgcgcat gcggaatcag
      781 ctcgggctcg tcgttctcca gatgcagatc ggacagcacg cgaatcttca cgacgacaac
      841 tccttcaatg ggcctcgcac cagatggcga aggctttgga ccaagggctt taaaccgaag
      901 ggctttaaac cgaagggctc taaaccgaag gccttaaacc aaaggcctta aaccaaaggc
      961 cttaaaccaa aggctctaaa ccctagcgta gcaccttgcc tggattcatc aggttcagcg
     1021 ggtcgagcgc gcgtttgacg gcgcgcatca gctgtacctc gacgtcctgc ttgtaatgca
     1081 tctcctcgtc gattttcaac tggccaagac cgtgctccgc gctgatgctg ccgcgatgcc
     1141 ggtgcacgct gtcgtagacg agctggttaa tcggactttg ccaggtcttg aggaacgcct
     1201 tcgcgtcgac gccctctggc gcctgcacgt tgtagtgcag gttgccgtcg ccgagatggc
     1261 cgaacgtgac catgcgcacg cccggcacgg cttgcgcgat cgccgcgtcg gtttcctcga
     1321 tgaagtggcc aatgcgcgaa atcggcaccg cgatatcgtg cttgatgttc aggccttcct
     1381 cagcctgcgc gagcgggatg tgttcgcgca gattccagaa tgcgcgcgac tgcgtgaggt
     1441 tttccgcgac caccgcgtct tccaccagac cttcttcgag cgcggtttcc atcaggcgct
     1501 cgaacagtcc gcgcgcgtgc tcctcgctct cgctgtctga cagttccagc agcacgactt
     1561 gcgcgtgcgg ttcggcgaag ggatagcgca tctgctcgaa atgccggccc accagccgca
     1621 gacagaaatc cgacatcagt tcgaagccgg tcagaagcgg cccggcgacg cgttgcgtga
     1681 gcgacaggaa atcgagtgcc gcatgcggcg acgcgagtgc ggcgagcgcc gtgacacgcg
     1741 cggcaggttg cggatgcagc ttgagcaccg cagcagtgat gatgccgagc gtgccttccg
     1801 cgccgatgaa cagatcgcgc aggtcgtagc cggtgttgtc cttgcgtagt ccgcgcaggc
     1861 cgtcccacag ttcgccttgc ggcgtaacca cttccaggcc cagacagagt tcgcgagtgt
     1921 tgccgtaacg cagtacgccg gtgccgcccg cgttggtggc gagattgccg ccgatcgtgc
     1981 aactgccttc cgcagcgagg ctcaagggga acaggcggcc ggcttgcccg gcgtgccttt
     2041 gcacgtcggc aaggataacg ccggcttcga ccgtgatcgt attgttgtgc ggatcgatat
     2101 cgcgcacacg gttcagacgc cgcagactga tgaccgcctg cgcgccgctt gcatccggcg
     2161 tggcgccgcc ggccagaccg gtgttgccgc cctgcggcac cagcgcgatg cgatgctcga
     2221 tggcgagttt gacgagcgcc gcgacctcgt caggcgtggc cgggcagagc accgcgcagg
     2281 ccgcgccggt gtagcggcgg cgccagtcgg tgaggtaggg ggcggtgtcg tgcggatcgg
     2341 tcagcacctg ggcggcgccg atgacgtcgc ggcaagcagt gaggaaagcg gcttgggtca
     2401 tgttggtcgg tcaggatgtt cggcggatag gttacgcggg aagcgctgtt ttcatcgcgt
     2461 gcctccctcg gcctgctcgg cgttcggctt ggtgttgtca g
//
LOCUS       DATASH010000439         5573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_660918,
            whole genome shotgun sequence.
ACCESSION   DATASH010000439 DATASH010000000
VERSION     DATASH010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5573
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660918"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..1366)
                     /gene="hmgA"
                     /locus_tag="VHV99_12145"
     CDS             complement(20..1366)
                     /gene="hmgA"
                     /locus_tag="VHV99_12145"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEX3636223.1"
                     /translation="MDTPTRTPNTASSRLEIEPGYQSGFANEFATEALPGALPEGRNS
                     PQRAAYGLYAEQLSGTAFTAPRAHNRRSWLYRIRPAAVHKPFTQLPSDRLVANFAEVP
                     PTPPNQLRWDALPMPTEPTDFVDGWVTMAGNGAAEAMSGCAIHLYAANRSMQDRFFYN
                     ADGELLIVPQEGRLHIATEMGQLDIGPFEIAVIPRGLRFAVSLPDGNARGYICENFGA
                     LLRLPDLGPIGSNGLANPRDFLTPHAAYEDREGDFELVAKMNGNLWRADIGHSPLDVV
                     AWHGNYAPYKYDLRRFNTIGSISFDHPDPSIFLVLQSQSDTPGVDSIDFVIFPPRWLA
                     AEDTFRPPWFHRNVASEFMGLVHGVYDAKAEGFVPGGASLHNCMSGHGPDADTFEMAS
                     HSDTSTPKKVGDTMAFMFETRTLIKPTRFALETAQLQAHYYECWQGLKKHFNPEQR"
     gene            complement(1415..2779)
                     /locus_tag="VHV99_12150"
     CDS             complement(1415..2779)
                     /locus_tag="VHV99_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3636224.1"
                     /translation="MSTKPAAAATNVIEVERVLGETHHPAFQLMLLALCGLCLVVDGF
                     DAQAMGYVAPSVIGEWHVSKAALGPVFSASLFGMLLGALGLSVLADRVGRRPVLIGTT
                     LFFALSMLATPFVTTIPALIALRFITGLGLGCIMPNAMALVGEFSTPAHRVKRMMLVS
                     CGFTLGAALGGFISAALIPAYGWRAVFWVGGAVPLLLAFAMLAALPESLQFLVLKGRS
                     DRALRWLARFNPALPIDAATRVVVREKGNGGAPVAELFRAGRGPVTLILWAISFMNLI
                     DLYFLSNWLPTVMRDAGYSPSTAVIVGTVLQTGGVIGTLLLGWFIERFGFVRVLFVCF
                     AGAALAVGTIGTVAHALPWLLIVVFAGGFCVVGGQPAVNALAGHFYPTTLRSTGIGWS
                     LGIGRIGSVIGPLVGGQLIALNWSNESLFHAAAVPVLCSAVLVIALAAATRQGERPSE
                     PQTA"
     gene            3132..3965
                     /locus_tag="VHV99_12155"
     CDS             3132..3965
                     /locus_tag="VHV99_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate phosphodiesterase"
                     /protein_id="HEX3636225.1"
                     /translation="MIPPTVSNLIARAADHPFLGEHLAMGTGVHSEIAVAQFDGVELA
                     SAYEPIFDISVHALAQSLSSGAEGVDRFGDELGFQAVTQRLDGAPFDVFDSFDRVADD
                     QDLVALDRMSRALHAINFFGAQRHGLLFLRVHERLLKSVKYDHGLHFSTVLVSFGLNP
                     SRVVIELPAAAVAHKTFLGYLTTSYQRYGFKVAGNLSNAGQILSVSETARLDFIKMDA
                     ATALRDGMVKPLVGYASRLRIPLIFNRVMDEPQFDALQQYDVRFVQGPLFNAHYHDRA
                     V"
     gene            complement(4044..4829)
                     /locus_tag="VHV99_12160"
     CDS             complement(4044..4829)
                     /locus_tag="VHV99_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX3636226.1"
                     /translation="MTPTSTLSDPLDERKFVVALARGLELLRAFKPGETMLGNRDFVE
                     RTGLPKATVNRLAYTLTVLGYLRLDETLGKYALDAGVLSLGFALLSGTDTLELARPHM
                     RTFAREVGAAVSLGCRDGLDMIYLETIRSETALTLGLASGSKLSMLTSSMGRAYLAVQ
                     PLDVRAALLAELKRSAGKEGAGLLADAEKEIESFAQERCCYSFRAWHDDVNAVAVPFR
                     EPREGRWLVLSCSGPASSMGEDVFRENVAPRLKALARRLGDTG"
     gene            4933..>5573
                     /locus_tag="VHV99_12165"
     CDS             4933..>5573
                     /locus_tag="VHV99_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX3636227.1"
                     /translation="MAEAAQFHWEDPLLLDQQLTEDERMVRDAAAAYSQDKLQPRVLE
                     AFRHEKTDIEIFREMGELGLLGPTIPEQYGGPGLNYVAYGLIAREVERVDSGYRSMMS
                     VQSSLVMVPIYEFGSESQKQKYLPKLATGEWIGCFGLTEPNHGSDPGSMVTRAKKVDG
                     GYSLSGSKMWITNSPIADVFVVWAKLEENGKDSIRGFILEKGWKGLSAPTIHS"
BASE COUNT         1086 a         1762 c         1745 g          980 t
ORIGIN      
        1 tggagatcgt tcatcgcgct catcgttgct ccggattgaa gtgtttctta agaccttgcc
       61 agcactcgta gtaatgcgcc tgcagttgcg cggtttcgag cgcgaagcgc gtcggcttga
      121 tcagcgtgcg cgtttcgaac atgaacgcca tcgtgtcgcc gactttcttc ggcgtcgacg
      181 tatcgctgtg cgaggccatc tcgaaggtgt ccgcgtccgg cccatgaccc gacatgcagt
      241 tgtgcagact cgcgccgccc ggcacgaaac cctcggcctt cgcgtcgtac acaccatgca
      301 cgaggcccat gaattcgctc gcgacgttgc gatggaacca gggcggccgg aacgtatctt
      361 cggcggcgag ccagcgcggc gggaagatca cgaagtcgat cgaatcgacg cccggcgtgt
      421 cgctttgcga ttgcagcacc agaaagatcg acgggtccgg atggtcgaag ctgatcgagc
      481 cgatcgtgtt gaagcggcgc aagtcgtact tgtacggcgc gtagttaccg tgccatgcca
      541 ccacgtcgag cggcgaatgg ccgatgtccg cgcgccacag attgccgttc atcttggcga
      601 cgagttcgaa gtcgccttcg cggtcttcat aggcggcatg cggcgtgagg aagtcgcgcg
      661 gattggcgag gccgttcgag ccgatcgggc cgaggtccgg cagacgcagc aacgcgccga
      721 agttctcgca gatatagccg cgcgcgttgc cgtccggcag gctcaccgcg aagcgtaggc
      781 cgcgcgggat caccgcgatc tcgaacggtc cgatgtcaag ctgacccatt tcggtggcga
      841 tatgcaggcg gccttcctgc ggcacgatca gcaattcacc gtcggcgtta tagaagaaac
      901 gatcctgcat cgagcggttc gctgcgtaca gatgaatcgc gcagccgctc atcgcttccg
      961 ccgcaccgtt gcccgccatc gttacccagc cgtcgacgaa atcagtcggc tcggtgggca
     1021 tcggcaaggc gtcccagcgc agctggttgg gcggcgtcgg cggcacctcg gcaaagttgg
     1081 cgacgagccg gtccgagggc aactgcgtga acggcttgtg aactgccgcc gggcgaatcc
     1141 ggtacagcca cgaacggcgg ttatgggcgc gcggcgcagt gaatgcggtg cccgacaact
     1201 gctcggcgta caagccataa gccgcgcgtt gcggcgaatt gcggccttcg ggcagcgcgc
     1261 ccggcaaggc ctccgtggcg aattcgttcg cgaatcccga ctgatagccc ggctcgatct
     1321 cgagccgcga gctggcggta ttcggcgtgc gggttggggt atccatacaa agttctccgt
     1381 tgatacttga atcggtcgac gtgagctttc tgcctcaggc tgtctgcggc tcggatggcc
     1441 gttcgccttg ccgggtcgcc gcggccagcg cgatgaccag caccgcggag cacagcaccg
     1501 gcaccgcggc ggcatgaaac agcgattcgt tcgaccagtt cagcgcgatc aattgtccgc
     1561 caaccagcgg tccgatcacc gaaccgatgc gcccgatacc caggctccag ccaatgccgg
     1621 tcgagcgcag tgtggtcgga taaaaatggc cggccagcgc gttgaccgcc ggctgtccgc
     1681 cgaccacgca aaagccgccc gcgaatacga cgatcaggag ccacggcagc gcatgcgcca
     1741 ctgtgccgat ggtgccgacg gccagcgccg cgcccgcgaa gcacacgaac agtacgcgca
     1801 caaaaccaaa gcgctcgatg aaccagccga gcagcaaggt gccgatcacc ccacccgtct
     1861 gcaacaccgt gccgacgatt accgccgtgc tcggcgagta gcctgcgtcg cgcatcacag
     1921 tgggcagcca gttcgacagg aagtaaagat cgatcaggtt catgaagctg atcgcccaca
     1981 gaatcaacgt gaccgggcca cggcccgcac ggaacagttc ggcgaccggc gcaccgccgt
     2041 tgcccttctc acgcacgaca acacgcgtgg ccgcatcgat cggcagcgcc ggattgaacc
     2101 tggcgagcca acgcaacgcg cgatcgctgc gccccttgag aacgaggaat tgcagcgact
     2161 ccggcaatgc cgccagcatc gcgaacgcga gcagcaaggg caccgcgccg cccacccaga
     2221 acaccgcgcg ccagccgtac gcaggaatca gcgccgcgct gatgaaacca cctaaagccg
     2281 cgcccagcgt gaagccgcac gacaccagca tcatgcgctt gacgcgatgc gcgggcgttg
     2341 agaattcgcc gaccagcgcc attgcgttcg gcatgatgca accaagcccg aggccggtaa
     2401 tgaagcgcag cgcgatcaat gcagggatcg tcgtgacgaa gggcgtggcc agcatcgaca
     2461 gggcgaagaa aagcgtcgtg ccgatcagga ccggacgccg gccaacacgg tcagccagca
     2521 ccgacaagcc caacgcgccc agcagcatgc cgaacagact ggcgctgaac acggggccga
     2581 gcgccgcctt cgagacatgc cattcaccga tcacgctcgg cgcgacgtag cccatcgctt
     2641 gcgcgtcgaa gccgtcgacc acgaggcata gcccgcatag cgcgagcaac atcaactgga
     2701 atgccgggtg atgcgtctcg ccgagcacgc gctcgacttc gatcacattg gtggcggccg
     2761 cggccggttt ggtgctcatt gagtgccctt catcctgagt ggattgcctg gtaggttgct
     2821 agataacggc gcgcgcccgg ttggcgatcg gaaaatgcac cctttcccgc cagggaaacg
     2881 gccacgcgat cgccatcgca gccgcgctgc aatttacgta tggtaaaact aattacgaat
     2941 agtgaaacta acgtataccc tgaaaacgcg cgtcagaagc cctcgcagcc ggttgcgaca
     3001 agcgtttgcc gataacgtta tgagcatttt gttcaagtgc gcgcggcgca gactgctacc
     3061 atcaaagaat gcggcaaccg agtcgcgctg ccgagcgccg agcgcgcacc tatcgccatc
     3121 cattcgccta catgattccg ccgaccgtct ccaacctgat tgcacgcgcc gccgaccacc
     3181 catttctcgg cgaacatctg gcgatgggaa ccggcgtgca tagcgagatc gcggtggctc
     3241 agttcgacgg cgtcgaactt gccagcgcct atgagccgat cttcgatatc agcgtgcatg
     3301 cgttggcgca gtcgttgtcg tcgggcgccg agggcgtgga tcgtttcggc gacgaactcg
     3361 ggtttcaggc ggtcacgcag cgtctggacg gcgcgccctt tgatgtcttc gattcattcg
     3421 accgtgtcgc cgacgatcag gatctggtcg cgctcgaccg catgtcacgc gcgctgcacg
     3481 cgatcaattt cttcggcgcg cagcgacacg ggctgctgtt tctgcgggtg cacgagcggc
     3541 tgctcaagag cgtcaagtac gaccatggcc tgcatttctc aaccgtgctg gtgtcgttcg
     3601 gactgaaccc gtcgcgcgtg gtgatcgagt tgccggcggc ggcggttgcg cataaaacct
     3661 tcctcggcta tctgaccacg agctatcagc gctacggctt caaggtggcg ggcaatcttt
     3721 cgaacgcggg gcagattctg tcagtgtcgg aaacggcgcg gctcgacttc atcaagatgg
     3781 atgcggcgac cgcgctgcgc gatgggatgg tgaagccgct agtgggctat gcaagccgct
     3841 tgcggattcc gctgatcttc aatcgcgtga tggatgagcc gcagttcgac gcgttgcaac
     3901 agtacgatgt gcgcttcgtt caagggccgt tgttcaacgc gcattatcat gatcgggcgg
     3961 tgtgaacccg cgttcgagca gactcgccgc ttactccggc tatcaactcc gcttcgtatc
     4021 gtagcgcggc gccgccctgc atctcagccg gtatcgccca gccgccgtgc caacgccttc
     4081 aggcgcggcg cgacgttttc cctaaacaca tcctctccca tcgacgacgc cggcccgctg
     4141 caactcagca ccagccagcg gccttcgcgc ggttcgcgaa acggcaccgc gaccgcattc
     4201 acgtcgtcgt gccacgcgcg aaacgaatag cagcagcgct cctgcgcgaa gctttcgatt
     4261 tccttttcgg catctgccag cagcccagcg ccctctttgc cggcgctcct cttaagttcg
     4321 gcgagcaacg cggcgcgtac gtccaatggc tgcaccgcca gatacgcgcg tcccatcgaa
     4381 ctggtcagca tcgacagttt cgagccggat gcgagtccga gcgtcaacgc ggtttcgctg
     4441 cgaatcgtct ccagataaat catgtcgagg ccgtcgcggc atccgagcga taccgccgcg
     4501 cccacttcgc gtgcgaaggt gcgcatgtgc ggacgcgcga gctcgagggt gtcggtgccc
     4561 gagagcagcg cgaaaccgag cgacagcacg ccggcgtcca gcgcatattt acccagggtt
     4621 tcgtcgagcc gcagatagcc gagcacggtc agcgtgtagg ccagacggtt gaccgtcgcc
     4681 ttcggcaacc ctgtgcgttc gacgaaatcc cgattgccga gcattgtttc gccgggtttg
     4741 aacgcacgta gcaactccag cccgcgcgcg agggcgacga cgaatttgcg ctcgtcgagc
     4801 gggtcggaga gagtagatgt gggcgtcatg gggtggtaca ctcggacgtg gtttgcaaaa
     4861 cattgtttcg catagcggaa cttaagtcaa gctcggacac gtttagtcaa gcaaggaatc
     4921 aggagatacg tcatggcaga ggccgcgcag tttcactggg aagacccgtt gctgctggat
     4981 cagcagctca ccgaagacga acgcatggtg cgcgacgccg ccgccgctta ctcgcaggac
     5041 aagctgcagc cgcgcgtgct cgaagcgttc cgtcatgaga agaccgacat tgagatcttc
     5101 cgcgagatgg gcgaactcgg cctgctcggc ccgacgattc ccgagcaata cggcggtccc
     5161 ggtctgaact acgtggcgta cggtctgatc gcgcgtgaag tggagcgcgt cgattccggc
     5221 taccggtcaa tgatgtcggt gcaatcgtcg ctcgtcatgg tgccgatcta cgaattcggc
     5281 tcggaatcgc aaaagcagaa gtacctgccg aagctcgcca cgggtgaatg gatcggctgc
     5341 ttcgggctga ccgaaccgaa ccacggctcc gatccgggca gcatggtcac gcgggcgaaa
     5401 aaggtcgacg gcggctattc gctgtcgggc tcgaagatgt ggatcaccaa ttcgccgatc
     5461 gccgacgtgt tcgtcgtgtg ggcgaagctc gaggagaacg gcaaggattc gatccgcggc
     5521 ttcattctcg agaagggctg gaaggggctg tcagcgccga ccatccatag caa
//
LOCUS       DATASH010000440         3457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2046969,
            whole genome shotgun sequence.
ACCESSION   DATASH010000440 DATASH010000000
VERSION     DATASH010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2046969"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..477)
                     /locus_tag="VHV99_12170"
     CDS             complement(136..477)
                     /locus_tag="VHV99_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat domain-containing protein"
                     /protein_id="HEX3636228.1"
                     /translation="MSVLVESSSSTAIRGAPVAALLVGAALFSPFCHADDITYGYDAL
                     GRLVSVTVAGNAAYYDYDAAGNTTAIRRQGALSSAVPVSGAGSSPASQDGELVSSPAS
                     TQASVRQAHSA"
     gene            complement(818..3265)
                     /locus_tag="VHV99_12175"
     CDS             complement(818..3265)
                     /locus_tag="VHV99_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018431959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX3636229.1"
                     /translation="MNPQVLEEAVLQPATRSALAELTTVPGVQAPPRRTALRSDKTVR
                     IAIVHDWLVTYAGAEKVLEQIVACFPDADLFSLVDFLDDRSFLRGKPVTTSFIQKLPL
                     ARTRYRAYLPLMPLAIEQLDVSAYDVVISSSHAVAKGILTGPDQVHISYVHSPIRYAW
                     DLQHQYLQQSKLTAGPKSAMARLILHYIRNWDIRTSNSVDGFVANSDFIARRIRKVYQ
                     REAQVIFPPVDVEAFSLCTDKDDFYLTASRMVPYKKIDLIVEAFARMPERKLVVIGDG
                     PDMQKIRAKAAPNVEIMGYQPFKVLKEKMSRAKAFVFAAEEDFGISVVEAQACGTPVI
                     AYGKGGALETVRDLSEPRPTGMFFDEQNAESIIAAVERFDDHVKRFSPLDCRANAEQF
                     SAANFRERFFAHVRASVPALRSATLPPYVPYKAEPGANAPRILAVDQSGVLGGAELSL
                     LEIVKALRSRIEVVLFDDGPFRTALAQVGVPVNVLDAGALRNVRKQGGSLPKGQALKG
                     LMSLVRATAKRARNAEVIYANTQRAMVVGAIAGKLARRPVVWHLRDIVSPEHFGGKQL
                     AIIKWCARLGLAHVIANSAASARAFAELTHFDEKRIDVVFNGISSAPFDALRTVSQAT
                     LRRRLNLPQDAFLVGSFSRLARWKGQHVLLEAMVLNPQMHAVLVGAPLFGEDAYEVEL
                     HAFVAAYNLGDRVHFLGFQHDIPACMCAVDAVVHTSITPEPFGRVIVEGMLAQRPVVA
                     ARAGGVLEIIDDYENGVLCTPADAHGLADTLAELRSNDDLRNRLVRNGYQTALRRFGT
                     ATYVEGVERILKRVAGR"
     gene            complement(3262..>3457)
                     /locus_tag="VHV99_12180"
     CDS             complement(3262..>3457)
                     /locus_tag="VHV99_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007745367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HEX3636230.1"
                     /translation="DMLAHLAFLLASLWLASFLPSLAVAIVAGLLMVGANVAWSITMT
                     PALRDLARSLLLRLNPRKSA"
BASE COUNT          710 a         1133 c         1036 g          578 t
ORIGIN      
        1 tcggcgcgcg catgcggcgc gacaaaccac gacatcagca cgaagaaaag cagcacgaac
       61 ggacgcaaca tcgcgcgacg cgccgcaagg gcatcgattg attgcataag aatctggctg
      121 gaacctggaa aaggatcagg cgctatgcgc ctggcgcacg ctggcctgag tcgaggctgg
      181 cgacgatacc aattcgccgt cttgcgatgc aggcgaagaa cccgcgccgc tcaccggcac
      241 cgctgaagac agggcaccct gccgccggat cgccgtcgta ttgccggccg cgtcgtagtc
      301 gtaatacgcc gcattgcccg cgacggtgac cgacaccagc cggcccaatg cgtcgtagcc
      361 gtaggtgata tcgtcggcat gacagaaggg agaaaacagc gccgcaccga caagcagcgc
      421 tgcgacgggc gcgcctcgga tggctgtcga cgaactactc tctacaagaa cgctcaccgc
      481 atctcctcgc aatcaatacg tattacaaac tgtttgcgaa caatacggca ctgacccttc
      541 ttgaagaatc agctggttca gtttgatacg taggaatatc ctgccctccg agtcggacag
      601 acctctcatt gacccgcgcc agaaacccaa agccgcgtcg aaacaggttt gaacgcaact
      661 cgataaaaag cgcgctaaaa aaaacggagc gaaggtccac aggactttcg ctccgctgcg
      721 ccaacggcag gaaccccggc cgagtccccg cctacccccg cgttaaatat tcgactcaac
      781 catcccaacc ctctgctcaa gcgcccgcgc acgctcccta ccgccccgcc acgcgcttca
      841 gaatccgctc gacaccttcc acatacgtcg cagtgccgaa ccgcctcagc gccgtctgat
      901 agccgttcct gaccagccgg ttacgcagat cgtcgttcga acgcaattcg gcgagcgtgt
      961 cggcgagacc gtgcgcgtcg gcgggcgtgc acagcacgcc gttctcgtaa tcgtcgatga
     1021 tttccagcac gccgcccgcg cgggccgcca ccaccggccg ctgcgccagc attccctcga
     1081 cgatcacgcg gccgaatggc tccggcgtga tcgacgtatg gaccaccgcg tcgaccgcgc
     1141 acatgcaagc cggaatgtcg tgttgaaaac cgaggaaatg gacccggtcg ccaaggttgt
     1201 acgcggcgac gaaggcatgc aattcgacct cgtacgcgtc ctcgccgaac agcggcgcac
     1261 ccaccagcac cgcatgcatc tgtgggttga ggaccatcgc ctcgagcagc acatgttgcc
     1321 ccttccagcg cgcgaggcgg ctgaacgaac caaccagaaa cgcgtcttgc ggcaagttca
     1381 ggcgccgccg cagcgtcgct tgcgaaaccg tgcgcaatgc gtcgaacggt gcagaagaaa
     1441 tgccgttgaa caccacgtcg atacgctttt cgtcgaaatg cgtgagttcc gcgaaggcac
     1501 gcgcggaagc cgccgaattg gcgatcacat gtgcgaggcc gagacgcgcg caccacttga
     1561 tgatcgccag ctgtttgccg ccgaagtgct cgggactcac gatgtcgcgc agatgccaga
     1621 ccaccggccg ccttgccagc ttgccggcaa tcgcgccgac caccattgcg cgctgtgtgt
     1681 tcgcgtagat cacctcggcg ttgcgcgcgc gtttcgcggt ggcgcgcacg agcgacatca
     1741 agcctttaag cgcctgcccc ttcggcaacg acccgccctg cttgcgcacg ttgcgcaatg
     1801 cgccggcatc cagcacgttc acaggcacgc caacctgggc gagtgccgtg cggaacggac
     1861 cgtcgtcgaa cagcacgact tcgatgcgcg agcgcaacgc cttaacgatt tccagcagcg
     1921 ataactcagc gccgccgagc acaccgctct ggtcgacggc aagaatgcgc ggcgcgttgg
     1981 cgcccggttc tgccttgtaa gggacgtacg gcggcagcgt cgccgagcgc aatgccggca
     2041 cggaagcccg cacatgcgcg aagaaacgtt cgcggaaatt cgcggcggag aattgctcgg
     2101 cgttcgcgcg gcaatccagc ggcgaaaagc gcttcacgtg atcgtcgaaa cgctcgaccg
     2161 cggcgatgat cgattcggca ttctgctcgt cgaagaacat gccggtgggc cgcggctccg
     2221 acagatcgcg cacggtttcc agcgccccgc ccttgccgta ggcaatcacc ggcgtgccgc
     2281 acgcctgcgc ttcgaccact gagatgccga agtcctcttc agccgcgaac acgaacgctt
     2341 tcgcgcggct catcttttcc ttgagcacct tgaacggctg gtagcccatg atctcgacat
     2401 tcggcgccgc cttcgcgcgg atcttctgca tatcggggcc gtcgccgatc accaccagct
     2461 tgcgctccgg catccgcgcg aacgcctcga cgatcaggtc gatctttttg tacggcacca
     2521 tccgcgaggc ggtcagatag aaatcgtcct tgtcggtgca gagcgaaaat gcttcgacat
     2581 ccacgggcgg aaagattacc tgcgcctcgc gctgatagac cttcctgatg cgccgcgcga
     2641 tgaagtcgga gttcgcgaca aagccgtcca ccgaattcga cgtgcggatg tcccagttgc
     2701 ggatgtagtg cagaatcagc cgcgccattg cggacttcgg cccggcggtc agcttcgact
     2761 gctgcaagta ctggtgctgt agatcccacg catagcggat cggcgaatgc acgtagctga
     2821 tatgcacctg atcggggccg gtcagaatcc cttttgcaac cgcgtgactg ctcgagatca
     2881 ccacgtcgta ggccgataca tcgagctgct cgatcgcgag cggcatcaag ggcagatacg
     2941 cgcggtacct ggtgcgcgca agcggcaatt tctgaatgaa cgaggtcgtc accggcttgc
     3001 cgcgcagaaa actgcggtcg tcgagaaaat cgacgaggct gaacaggtcg gcgtccggaa
     3061 agcacgcgac gatctgctcg agcaccttct cggcgcccgc ataggtgacg agccagtcgt
     3121 gcacgatcgc gatgcgcacc gtcttgtcgc tgcgcagcgc cgttcggcgc ggcggcgcct
     3181 gaacgccggg tacagtggtg agttcagcca gggcactgcg cgtcgcgggt tgcagcacgg
     3241 cttcttcaag gacttggggg ttcatgcgct tttcctcgga ttcagtcgca gcaggagtga
     3301 acgcgcaaga tcgcgtaagg cgggtgtcat cgtgatcgac caggcgacgt tcgcgcccac
     3361 catcaacagg ccggcgacga tcgccacagc gagactcggc aggaagctgg caagccagag
     3421 gctggccagc agaaaggcaa ggtgggcgag catgtcg
//
LOCUS       DATASH010000441         2328 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6596509,
            whole genome shotgun sequence.
ACCESSION   DATASH010000441 DATASH010000000
VERSION     DATASH010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6596509"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..548
                     /locus_tag="VHV99_12185"
                     /pseudo
     CDS             1..548
                     /locus_tag="VHV99_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015004177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            complement(590..2166)
                     /locus_tag="VHV99_12190"
                     /pseudo
     CDS             complement(590..2166)
                     /locus_tag="VHV99_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612036.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          400 a          737 c          738 g          453 t
ORIGIN      
        1 tccccaaaac ccgcgagctc tatcgcgagg tctgcgcgct gctgttcttc cgccacggca
       61 tcacgccgac cgccaaccgg ctgtatcagc tcgtgaagcg gggcagcatg gggacgccga
      121 ccgccgtgct cggcgagttc tggacggaac tgcgcgagaa gagccgcgtg cgcatcgagc
      181 atccggacct gcccgctgat cttggtgcgg cggccggcga actggtcgcg accctctgga
      241 cgcgcgccac tgcgtcggcc gacaccgcgc tcgacgcgct ccgtggcgaa gtcgaggcgc
      301 agcgggccga ggcgcagcag tcggtggccg ccgcccgcga cgaactgggt cgcgtcgaga
      361 cggcgctcga acagcgcacc gcggcgctgc tggccgcgca ggtcgaggtc cgggagctgg
      421 acagggcgca ggccgaacgg catgcgcagc gacaggcgct cgagggggaa ctggcgcgca
      481 tcagtgcggc gctggctgcg cgcgatcgcg aacttcaggc ggtgagggaa gggttttcgc
      541 gcgacctggc ggccgttgtc aaagtagttg tcgcctcgac gattgggatt caggctgctt
      601 tgagctcctg gttgtggggt tgtgagatga cgctgtccgg gtcagcacga tcgggattca
      661 ggtgcacgac ctgaatgggc tgccagttgc gcgtgggacc tttccagcgc agcggattac
      721 gctggcgtgc gctctcgtag agcgccgctc gtcgattcag gatgtcctga tcaaggttcg
      781 catggcgctg agccggcgtc acgaaacgga tcgcactgtg acgatgttcg tggttgtacc
      841 agcgcaccag tccggccacc caggtacgcg cggcgaacag ggtatcgaac acctccagcg
      901 gataggcggg ccggtatttc agcgttttga acagcgattc cgaaaacggg ttgtcgttgc
      961 tcactgccgg tcgactcaat gacggcatga cgcccagggc ctgcaaggtg gccagcatcg
     1021 tggtgccctt catcggcccg ccgttgtcgg aatgcaagat cacctgaccg ggccgtattg
     1081 cttcacgcgc gcagatatcc ttgaggactt cgctagcctg ggcgctactc tcctcggcat
     1141 agacttgcca gccgacgatc ttgcggctga acacgtccat gaacaaatag agatagaaat
     1201 attgtccccg gatcgttgct ggcaggtaag tgatgtccca gctgtacagc tggttcggcg
     1261 catcggcaca gaccgcgcgg ggcttgctgc gcgtgttggc cggccgctca ctgcgccggt
     1321 gtgcgagctg cttctcctcg cgcagcaccc ggtagaaagt cgattccgag gcgatgtagc
     1381 gttgctggtc ggcaaggcgt ggaacgatct ggctcggtgg cagatgaccg aattcggccg
     1441 aattcgccac cgccagcagc tcggcacgct cggcggcgct cagcttgtgg ggtggctcat
     1501 agcaccgtag cgaacgccca tccactgcat caggctcgcc gccctgccag cgctgaaccg
     1561 tacgggcgct cagccccagg acctcacacg cgcgtgcctg gcgagcccca gccgtggtcg
     1621 cctcgctgat cagaccgagc aatgtcttgc gctcttcgag ggccgtcatt cggcctcgtc
     1681 cccgaacagc gcacgatact ttttttgcag caccagcagc gccgccgcct ccgccagcgc
     1741 tttctcctta cggttcagtt cacgctgaag ctgtacgttg gcgtgcttca gttcgcgtac
     1801 ttcctgggcg ttctcacgcc ggctgccggc tgcgccaacg gcgcaaaagt ccacgcgcca
     1861 ctgcgacaga tgatgagcaa acaggccccg ttcacgacac cacgcgttca gcgcttcgtc
     1921 gagcagtcca tggctctgct gcaaggccag cagacgctct tccagtgacc agtcctccgg
     1981 tcgttttgcg cgcccagagt cttcgctccg agctgatgcc gcggcacctt tcatccagtt
     2041 cttcaaggtc attacgttca tgttcaattc atctgccacg gatccaatgg tgcgacttcc
     2101 gcggtgtaag accttcaaca gcgcctgctc tttgaactcg acagaatacg tttgtttcac
     2161 ctgcaacttg cacctctgat ctctttaaat taagaaaatt cagaggcgac aactattgtg
     2221 acgcccgggg gattcaggcc gccaagtgcg gattgggagt cgcagcagtg ccgagaatgc
     2281 tcgtccggga cgaactggac tcagctggtc tcgtcgcgcc gtttggct
//
LOCUS       DATASH010000442         2515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1850219,
            whole genome shotgun sequence.
ACCESSION   DATASH010000442 DATASH010000000
VERSION     DATASH010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1850219"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="VHV99_12195"
     CDS             complement(<1..837)
                     /locus_tag="VHV99_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008354264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEX3636231.1"
                     /translation="MTPQREETGADHWQDEPPFPVVPERNFAAQRMTLRVLLIAAIVL
                     PCLYVAAMAWSDLKSREADAVEVVDRNAHVAEEHTLKVFDMNETLNARVIDLIHGVDD
                     DTLRGEELDIHNKLVAMGGGYPQVASVSIIGADGRLIATSRIYPVPDVSIENRDDFIG
                     IRDHKMFENVSRVMFGRVAGETVFNTAMPRKNSDGRFAGIVTIALRPSYFSAFYRELL
                     GEHTTVSLGLTRADGAVLAWYPTRPPNRMELAASSPLREAYTLEQHNGVVRMRSGLDN
                     EMK"
     gene            1443..2288
                     /gene="hpnD"
                     /locus_tag="VHV99_12200"
     CDS             1443..2288
                     /gene="hpnD"
                     /locus_tag="VHV99_12200"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HEX3636232.1"
                     /translation="MAVSNLIADDPAPDVAKVTSGSSFYLAMRILPAAQRDAMYQVYA
                     FCRAVDDIADGTLPCGERAAALEQWRADIDDCYAGKPRRSLAVLTQHIEAFHLSRDDF
                     IAVIDGMAMDAAEDIVAPDEATLDLYCDRVASAVGRLSVRIFGMHEDAGRRLAHHLGR
                     ALQLTNILRDIDEDAGIGRVYLPRELLSREGISTTDIASIVNDARLPRVCLTLADRAR
                     SHYAQSDAIMATAPRAQVRAPRIMSGAYRCVLEANLKRGFDLPRNHVRTPRSRLLWIV
                     ARHIF"
     gene            2288..>2515
                     /locus_tag="VHV99_12205"
     CDS             2288..>2515
                     /locus_tag="VHV99_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3636233.1"
                     /translation="MSRLVHVIGAGLSGLAAAVQLQRRGARVMLHEAAPHAGGRCRSY
                     FDIKLNATIDNGNHLVLSGNHATLSYAKAIGA"
BASE COUNT          464 a          753 c          783 g          515 t
ORIGIN      
        1 cttcatttca ttgtcgagcc ctgagcgcat tcggaccacg ccgttgtgct gttccaaggt
       61 atacgcctcg cgcaatggag aactggcggc gagttccatg cggttgggcg gcctggtcgg
      121 ataccaggcg agcacggcgc cgtctgcgcg tgtgaggcca agcgatacgg tggtgtgttc
      181 gcccagcaac tcgcgataga acgcgctgaa gtaactcgga cgcagcgcaa tggtcacaat
      241 gccggcgaag cggccatccg agttcttgcg cggcatggcc gtgttgaaca cggtttcccc
      301 cgccaccctg ccgaacatca cgcgggagac gttttcgaac atcttgtggt cgcggatgcc
      361 gatgaagtcg tcgcggtttt cgatcgatac atccggcacg gggtaaatgc ggctcgtggc
      421 aatcaaacgg ccatcggcgc caataatgct caccgacgcc acttgcggat aaccgccgcc
      481 cattgccacg agtttgttgt gaatgtcgag ttcttcgccg cgcaaggtgt cgtcgtccac
      541 gccgtggatc aggtcgatca cgcgggcgtt gagcgtctcg ttcatgtcga agactttcag
      601 cgtgtgttct tcggccacgt gcgcgttgcg atcgaccacc tcgacggcat cggcttcgcg
      661 cgatttcagg tcgctccacg ccatggccgc aacatacagg cagggcagca cgatggcggc
      721 gatcagcagg acccggaggg tcatgcgctg ggcggcgaag ttacgctccg gcaccacggg
      781 aaacggcggc tcgtcctgcc aatggtcagc gcccgtttcc tcgcgttgcg gcgtcatgat
      841 gactcgtcgt cccgatgtga attggctgag cgtggcatct ttatgcaacg atgaaggcag
      901 cgaatgcggc acgcggaatt tcgcgcattt tagccgcttg ttggtttgcc gccggcgctc
      961 gggaaagaga aggcgtgcat aaatcgtgcc tttatcgtcc gtttaaccga tgaatcgata
     1021 ttctgccaaa ccgacggcat cgacgtttaa agcgcaggga ttgaggaaag atgaactctc
     1081 gggaggctgc gggcaggagg taaaaaagcg gcgaaccggg taaagttggc gggaaatatc
     1141 gcactgatcc agagcgagcg taaagaagga atcgaaggaa tccggtgaca gccctcgttt
     1201 tgtacgaaga ttctggctgc atcgcaggcg tgttgaaagc acgcggcagg ccgccttagg
     1261 gcaacagttc acaaacttga tatacttttc gccgcttccg gcatggcttg cacgccgcct
     1321 gaagccggtg tccaagccat gccgggcttg cactcagttt tacagcatgt tccatgaagc
     1381 cggatacccg gcgcccgatc taaacatttg ctgcccttgg gggcgccaca gcggagtccg
     1441 tcttggccgt ttccaatctt atcgccgacg atcctgctcc cgacgttgcc aaggtaacgt
     1501 cgggtagttc gttctatctc gccatgcgca tcctgccggc tgcgcagcgt gacgccatgt
     1561 accaggtcta cgcgttctgc cgggcggtgg acgatatcgc cgatggcacg ttgccgtgcg
     1621 gcgaacgcgc tgccgcgctc gaacaatggc gtgcggacat agacgattgc tatgccggca
     1681 agccgcgccg gtcgctggcc gtgttgacgc aacatatcga ggcgtttcat ctttcccgcg
     1741 atgacttcat tgccgtgatc gacggcatgg ccatggacgc ggccgaagac atcgtcgcgc
     1801 ccgacgaagc caccctcgac ctgtattgcg atcgcgtggc gagcgcggtc gggcggttat
     1861 cggtgaggat attcgggatg cacgaagatg ccggacggcg tcttgcgcac cacctcggcc
     1921 gtgcgcttca actcacgaac attcttcgcg acatcgatga agacgccggc atcgggcgtg
     1981 tttatctgcc gcgagaactg ctgtcccgcg aaggtatttc cacgaccgac atcgcgtcaa
     2041 tagtcaacga tgcccgcttg ccgcgcgtct gcctgacgct cgcggatcgc gcgcgcagcc
     2101 actacgcgca atccgacgcg atcatggcga ccgcgccgcg cgctcaggtg cgggcgccac
     2161 gcatcatgtc gggtgcgtac cggtgcgtgc ttgaggcgaa tttgaagcgt ggtttcgatc
     2221 tcccgcgcaa ccacgtccgc acgccgcgct cgcggctgct gtggatcgtc gcgcgacata
     2281 ttttctgatg tcgcgtctcg ttcatgtgat tggcgccggg ttgtcgggac tcgccgccgc
     2341 agtccagttg cagcgtcgtg gcgcgcgcgt gatgctgcac gaagccgcgc cacacgcagg
     2401 tggccgctgc cgttcttact tcgatatcaa gctcaacgcc acgattgata acggcaacca
     2461 tctggtgctg tcgggcaacc acgccacgct gagctatgcc aaggcgattg gcgcg
//
LOCUS       DATASH010000443         3782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5016119,
            whole genome shotgun sequence.
ACCESSION   DATASH010000443 DATASH010000000
VERSION     DATASH010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5016119"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VHV99_12210"
     CDS             complement(<1..315)
                     /locus_tag="VHV99_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015001901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HEX3636234.1"
                     /translation="MTSATSSAHVGATGGGVAVGRDTQSGFALGLPPRPALLSRRAWL
                     ALIALIIVFGLGVPFATLVVPETSALHLSAYATTITGKFMCYAIAALALDLVWGYCGI
                     LSL"
     gene            complement(317..1942)
                     /gene="urtB"
                     /locus_tag="VHV99_12215"
     CDS             complement(317..1942)
                     /gene="urtB"
                     /locus_tag="VHV99_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HEX3636235.1"
                     /translation="MAFSFPKFARSCIGGLTLALILSAPLAAFALTPADVAPLAGDDF
                     DAKSAAIDKLIANHDKESLAILKALSEDSALATDSGGILLQDGDTARDAVTGKTVAAG
                     DAQPVTLNNLLRSKVAGALSGLQLDSPDSATRDAAITALLQNPDPSIKPLVDAARARE
                     TDPALKKRLDTLWAMTALHDADPAKRLEAVQLVAARHDLDMNELLRPLVAKKPDGSFN
                     EADERVRAAAQSGIDELDSIQRRSQIAGTLFAGLSLGSVLLLAALGLAITYGLIGVIN
                     MAHGEFLMIGAYATYVVQNLFQRFAPGAFDWYPLLAVPASFAAAGLVGIVLERLVLKH
                     LYGRPLETLLTTFGVSLILIQATRMLFGAQNVQVINPAWMSGGVTVLPNLILPYNRLA
                     ILAFSLIVVGIAWAVLTKTRLGLFVRAVTQNRRMAACVGVKTARVDSYAFAFGAGIAG
                     LGGCALSQIGNVGPDLGQSYIIDSFMAVVLGGVGQLAGTVIGGFGLGLVSKAIEPFWG
                     AVLAKIAVLVLIVLFIQKRPQGMFALKGRSAEA"
     gene            complement(2054..3352)
                     /gene="urtA"
                     /locus_tag="VHV99_12220"
     CDS             complement(2054..3352)
                     /gene="urtA"
                     /locus_tag="VHV99_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033219 - urea binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HEX3636236.1"
                     /translation="MKRRSLLKFGSMSGALALAGQVPFAQAQSGSGPIKVGVLHSLSG
                     TMAISETSLKDTALMTIADINKNGGVMGRQLQPVVVDPASNWPLFAEKARQLITQEKC
                     AVVFGCWTSVSRKSVLPVFEELNGLLFYPVQYEGEEMSRNVFYTAAAPNQQAIPATEY
                     LMSTEGGGAKRFFLLGTDYVYPRTTNKILRAFLKSKGVQEADIQEVYTPFGHSDYQTI
                     VANIKTFSQGGKTAVISTVNGDSNVPFYKELGNQGLKASDVPVVAFSVGEEELRGIDT
                     KPLVGNLAAWNYFMSLRNPTNEKFKKQWAAWVAQNNLPGGTKRVTNDPMEATFVGIHM
                     WKQAVEKAKSTDVDKVRVAMIGQTFAAPSGFTLEMDGNHHLHKPVMIGEVRADGQFNV
                     VWRTKTAIRAQPWSPFIAGNSSKPDVVSSIPAFLKRSRVA"
BASE COUNT          690 a         1184 c         1202 g          706 t
ORIGIN      
        1 caggctgaga atgccgcagt agccccacac cagatcgagt gccagcgcgg cgatcgcgta
       61 gcacatgaac ttgccggtga tcgtcgtcgc ataagcggac agatgcagcg cgctcgtctc
      121 gggtaccacc agcgtcgcga acggcacgcc gagaccgaac acgatgatca gggcgatcag
      181 tgcgagccac gcacggcgcg acagcagcgc ggggcgcggc ggcaagccga gtgcgaagcc
      241 ggactgcgtg tcgcgtccga cagcgacgcc gccgcccgtt gcgccgacgt gagccgatga
      301 ggttgccgat gtcatctcat gcctccgcgc ttcggccctt gagggcgaac atgccctgcg
      361 gacgcttctg gatgaacagc acgatcagca cgagcaccgc gatcttcgcg agcaccgcgc
      421 cccagaacgg ttcgatcgct ttgctgacga gcccgagacc gaagccgccg atcactgtgc
      481 cggccagttg gccgacaccg ccgagcacca cggccatgaa cgaatcgatg atgtagctct
      541 ggccgaggtc gggtccgaca ttgccgatct gcgacaacgc acagccgccc aaacctgcaa
      601 tgcccgcgcc aaacgcgaac gcatacgaat cgacgcgcgc ggtcttcaca ccgacgcacg
      661 cggccatccg ccgattctgc gtgacggcgc gcacgaacag ccccaggcgc gttttagtca
      721 gaacagccca cgcgatcccc acgacgatca acgagaacgc gagaatcgcc agccggttgt
      781 acggcaggat cagattgggc aacacggtca cgccgccgct catccacgcc gggttgatca
      841 cctgcacgtt ttgcgcgccg aacagcatgc gtgttgcctg aatcaggatc aggctgacgc
      901 cgaaggtagt cagcagcgtt tcgagcggac ggccgtacag atgcttcaac accagccgtt
      961 cgagcacgat gccgactaac ccggctgccg cgaatgatgc cggcacggct agcagcggat
     1021 accagtcgaa cgcgcccggc gcaaaacgct ggaacaggtt ctgcacgacg taggtggcgt
     1081 aggcgccgat catcaggaat tcaccgtgcg ccatgttgat gacgccgatc agcccatagg
     1141 tgatcgcgag gccgagggcg gcgagcagca gcacgctgcc gagcgacaag cccgcgaaca
     1201 gcgtgccggc aatctggctg cgacgctgga tcgaatcgag ttcgtcgatg ccgctttgcg
     1261 cggcggcgcg cacgcgttca tccgcttcgt tgaagctgcc gtccggcttc ttcgcgacca
     1321 gcgggcggag cagttcgttc atgtcgagat cgtggcgcgc ggcgaccagt tgcaccgcct
     1381 cgagccgctt ggcggggtcg gcgtcgtgca acgccgtcat ggcccatagc gtgtcgagac
     1441 gcttcttcaa tgccgggtcg gtttccctgg cgcgagccgc atcgacgagc ggtttgatcg
     1501 acggatcggg gttttgcaag agcgcggtga tggcggcgtc gcgggtggcg ctgtcgggcg
     1561 aatcgagttg caggcctgac agtgcgcctg ctactttcga gcgcagcagg ttgttcagcg
     1621 tcacgggttg cgcgtcgccc gcggcgaccg ttttgccggt gacggcatcg cgtgcggtgt
     1681 cgccgtcctg cagcaggatg ccgccggaat cggtagcgag cgcgctgtcc tcggagaggg
     1741 ctttgaggat tgcgagcgat tctttgtcgt ggttggcaat cagcttgtcg atggccgcgg
     1801 attttgcgtc gaagtcgtcg ccggcgagcg gcgcgacatc ggccggggtg agcgcgaagg
     1861 cggcaagcgg cgcgctaagg ataagcgcga gcgtgagacc gccgatgcag cttcgtgcga
     1921 atttcggaaa tgaaaacgcc atggtggcct ttgaagaaaa ccggcagggc ggcgcggcat
     1981 cgtgctaacg agccgcttgc cgcaccgtcg ggatactgcg cgcggaggcg cgccgtcgtc
     2041 gcgatccgtc ctgtcaggca acgcgcgaac gcttcaggaa tgcgggaatc gaactgacca
     2101 catccggctt gctcgaattg ccggcgatga acggactcca cggttgcgca cgaatcgcgg
     2161 ttttggtccg ccacaccaca ttgaactggc cgtcggcgcg cacttcgccg atcatcaccg
     2221 gcttgtgcag atggtgattg ccgtccattt cgagcgtgaa gcccgacggt gccgcaaacg
     2281 tctggccgat catcgccacg cgcaccttgt ccacatccgt gctcttggct ttctcgaccg
     2341 cctgcttcca catatggatg ccgacgaacg tcgcttccat tgggtcattg gtcacgcgct
     2401 tggtgccgcc cggcaggttg ttctgcgcga cccatgccgc ccattgcttc ttgaacttct
     2461 cgttggtcgg gttgcgcagc gacatgaagt agttccatgc cgcgagattg ccgaccagcg
     2521 gcttcgtatc gatgccgcgc agttcttctt cgccgaccga gaacgcgacg accggcacgt
     2581 cggacgcctt cagcccctgg ttgcccagtt ccttgtagaa cggcacgttc gagtcgccgt
     2641 tgacggtcga gatcaccgcg gtcttgccgc cctgcgagaa ggtcttgatg ttcgcgacga
     2701 tggtctgata gtcgctgtgg ccgaacggcg tgtagacctc ctggatatcg gcttcctgca
     2761 cgcctttcga cttgaggaac gcgcgcagga tcttgttggt cgtgcggggg tacacataat
     2821 cggtgcccag caggaagaag cgcttggcgc cgccgccttc cgtgctcatc agatattcgg
     2881 tggccggaat cgcctgttga ttcggcgccg cggccgtgta gaacacgttg cgcgacattt
     2941 cttcgccttc gtattgcacc ggatagaaca gcaggccgtt cagctcttcg aacaccggca
     3001 gcaccgactt gcgcgacacg gaagtccagc aaccgaacac gacggcgcac ttttcctgag
     3061 tgatcagctg gcgcgctttt tcggcgaaca gcggccagtt cgacgccggg tccacgacca
     3121 ccggctgcaa ctggcggccc atgacgccgc cgttcttgtt gatgtccgcg atggtcatca
     3181 acgcagtgtc tttgagcgat gtctccgaga tcgccatcgt gccagacagg gaatgtagaa
     3241 cgcctacttt gatcggaccg ctacccgatt gcgcctgagc aaacggaacc tgacctgcaa
     3301 gcgccaatgc gcccgacatg gagccgaact tcaacagact acgacgtttc atgtatttcc
     3361 ccttgccaat gatggtgact gtttttgggc gtcgatttca gccagcaggg cttgtcccgc
     3421 atgacttgcc gcttgtgtac tgcaaggcac atgccatgta gtaaattcga cttgaagtcc
     3481 gcgtcttgcg tgctgcgacg tggcgatgcg gtgcctcgct tatgcggaac gcagcgcgaa
     3541 attggtgcag cagcggggta tgcgcctcgt tcaggtgcgt gtcgcgccgc aacgaagcgt
     3601 gtccatcggc cagcgggcct gagcggtgca tgaccgccgc gcctttgtgc atagggcggc
     3661 ctctgcctac gctgaaagag cactcacacg aggagacgcg atatgaatga gcgcgccagc
     3721 gtcaacccac ccgatatgtc cgctttctgg atgcccttta ccgcgaaccg tcggttcgag
     3781 ag
//
LOCUS       DATASH010000444        13240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5807251,
            whole genome shotgun sequence.
ACCESSION   DATASH010000444 DATASH010000000
VERSION     DATASH010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 13240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13240
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5807251"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="VHV99_12225"
     CDS             complement(<1..863)
                     /locus_tag="VHV99_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEX3636237.1"
                     /translation="MSIRVALNHVTHYRYDRLVALSPQVVRLRPAPHCRTPILSYSMR
                     VEPAGHFINWQQDAFANYQARLVFPEKTREFKVTVDLVAEMAVYNPFDFFLEPSAEQF
                     PFEYAPGLALELAPYRVKRPMTPRFAGFVASIDRTPMGTADFLVGLNQRLQREIRYLI
                     RMEPGVQTPEETLMSAAGSCRDSGWLLVETLRQLGLAARFVSGYLLQLAPDVKSIDGP
                     SGTEVDFTDLHAWCEVYLPGAGWLGLDPTSGLLAGEGHIPVACTPEPGSAAPISGAVD
                     ESEVEFVHTMS"
     gene            1264..2328
                     /locus_tag="VHV99_12230"
                     /pseudo
     CDS             1264..2328
                     /locus_tag="VHV99_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403773.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
     gene            complement(2897..4174)
                     /locus_tag="VHV99_12235"
     CDS             complement(2897..4174)
                     /locus_tag="VHV99_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3443 domain-containing protein"
                     /protein_id="HEX3636238.1"
                     /translation="MRDSASFINLLCALLLALLVSACGGGGGGSSSSGSAANSGNTTS
                     VSPGPTVTNTAPSPQVLAANAVRVTVDSGVSNVPNMPFVSITICAPGATQCQTIDHIL
                     VDTGSWGVRVFASQLPASMTLPQQKDGSGNLVAECMQFFDGYTWGSVKLADLQIAGEK
                     AASLPIQVIDPNYASLPSDCATYGASRNTPGTLQANGILGIGVFKHDCGANCVQQAVA
                     GTYYGCNGAACTSIPLAEALQVANPVPYFTTDNNGSMLSLPVVSGGAQSVTGQLVFGI
                     GTQGNNGLGSAQVVGVSPSNGTFTTVQNGTSYSNSILDSGSTGLFFQTSALPVCASPN
                     SAYYCPVSIESLSAMIEGVNGMTSTVNFAVGNATTISQTYSGDSALPLLAGPAFVTSS
                     IFDWGLPFFYGRNVYAAVEQQPTPGGTGPYVAY"
     gene            complement(4186..4635)
                     /locus_tag="VHV99_12240"
     CDS             complement(4186..4635)
                     /locus_tag="VHV99_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2844 domain-containing protein"
                     /protein_id="HEX3636239.1"
                     /translation="MLFTSIARAMLGASCALSFAAHATLGQNVNTVDSDQTRLRAVAR
                     TATTQNAYSVHLMTMPSGTLVREYVAPNGMVFGVAWQGPTLPNLKAVLGASFDQYVAA
                     IATRRGTPLAVSSRDLVVYSGGHLRAFAGYAYLPQAVPAGADVGVIQ"
     gene            complement(5262..5690)
                     /locus_tag="VHV99_12245"
     CDS             complement(5262..5690)
                     /locus_tag="VHV99_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEX3636240.1"
                     /translation="MSQPDAANPFGDFTKMLEQFKLPGFDVPAIMEARRKDMEALVQA
                     NQTAFQGMQSLAQKQTEMLRATLAELQSLTGQLSAGGAAPPAKAAELIQQSLHKSLAD
                     MQQLAQAAYQTQAESYAVIAKRVEENVQELKSLLQQQQKK"
     gene            6107..6550
                     /locus_tag="VHV99_12250"
     CDS             6107..6550
                     /locus_tag="VHV99_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636241.1"
                     /translation="MKKGLVSAALAAVLTVAATGAWAQEAAPSPASAHARGEPRRGDE
                     AHHPHGPMGPRFAVIHDLKQLRRLYAMSGREGEIVAVYHEVLNKTQDPMLRHYVYDAL
                     AREQLKPANTDQAIATLRTGLSEDLVAATKPRGMPAGMQPPAPPQ"
     gene            complement(6882..7568)
                     /locus_tag="VHV99_12255"
     CDS             complement(6882..7568)
                     /locus_tag="VHV99_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX3636242.1"
                     /translation="MKPFIVVHMMSSLDGRSLTDGWHLDFASDLYENTAATFKADGWI
                     CGRVTMQEISHGTDYPKGLAKGAVPRTDHFVERNAQQYAISIDPRGRVAWKSNTALKS
                     HIVEVLTEQVADDYLAYLQSIGVSYVFGGKSEIDLNKVVQTLARELGVKKLIVEGGSH
                     VSGAFVNAGLADEVSVLILPLVDGRSEHPSSFEVAMDKWQAPAYLKLESVEKTGNDAV
                     WLRYTKSVRA"
     gene            complement(7646..8743)
                     /locus_tag="VHV99_12260"
     CDS             complement(7646..8743)
                     /locus_tag="VHV99_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010108506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HEX3636243.1"
                     /translation="MSALFQPYKLKDVSLRNRIAIPPMCQYTAEDGLINDWHRVHLAG
                     LARGGAGLVIVEATAVSPEGRITPGCAGLWTDEQAQAFAPVVASIKAAGAVPGIQIGH
                     AGRKASANRPWEGDDHIADTDPRGWQTLAPSAIAFGGGLPKVPKAMTLDDIARVREDF
                     VAAARRARDAGFEWLELHFAHGYLGQSFFSAHSNQRDDAYGGSLENRSRFLLETLAAV
                     RKVWPEHLPLTARFGVIEYDGRDEETLAESIELAKNFKREGLDMLSVSVGFSTPEAAI
                     PWAPAFLAPIAERVRREAGIAVSSAWGIDTPELAERSVKNEQLDLVMVGRAHLADPHW
                     PYHAARKLGIERPSWTLPAPYAHWLERYAVA"
     gene            8831..9160
                     /locus_tag="VHV99_12265"
     CDS             8831..9160
                     /locus_tag="VHV99_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3636244.1"
                     /translation="MRTYTHPAAEDYTLGRLFHALSDPVRLEIVRRLSTIDEATCGDL
                     DGGRPKSSVSHHFRILREAGLVRTRVAGTVHQNSLRRAELDARFPGLMEAIVKQIVNE
                     PALTEPA"
     gene            complement(9229..9660)
                     /locus_tag="VHV99_12270"
     CDS             complement(9229..9660)
                     /locus_tag="VHV99_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3636245.1"
                     /translation="MSRTPSISQRWSAREVRILHQVPRVLVVDDNANAAEALATYLSY
                     EGVEARTANGCSEALRCVKDWVPDIVVLDIMMPERDGYETASALRSYLPTQSLGIVAF
                     TSLDEDHVKETGKARHNFDGYCQKGTAPTALLALMRILWRE"
     gene            10430..11497
                     /locus_tag="VHV99_12275"
     CDS             10430..11497
                     /locus_tag="VHV99_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007730163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3636246.1"
                     /translation="MENRALIIGASGIVGNNLADRLLATGWSVSGLSRGRTAVSPAIE
                     SITADLTSPESVSHALAGRAFSHLFFTAWARQATEKDNIRVNGGMVRHVLDTLGPAGG
                     VSHVALVTGLKHYLGPFEAYATGAVPVTPFREEQGRQPVDNFYYEQEDRLFEAAQRYG
                     FSWSVHRPHTIIGFAVGNAMNMGVTLAVYATLCKQEGKPFLFPGSAAQWDSLTDMTDA
                     RLLARHLEWAATSANARNEDFNVVNGDVFRWKWMWSRIADYFGIEAIPFDGVVRPLES
                     RMQDAQQQWSGIAARFGLIEADIGKLVSWWHTDADLGRPMEVLTDMTKSRKAGFLDYQ
                     STPDAFFALFDRLKAERIIPA"
     gene            complement(11600..12913)
                     /locus_tag="VHV99_12280"
     CDS             complement(11600..12913)
                     /locus_tag="VHV99_12280"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3636247.1"
                     /translation="MSTSGLKTIAAHDVERSAIRKTAVRIVPIVVLLYIVSYVDRINI
                     GFAALTMNGDLGIASNVFGLAAGIFFIGYFLFELPSNLLMVRFGARRWIARILLTWGL
                     LSCATAFVAGEKSFLVLRFLLGAAEAGFFPGIIFYLNDWFPARYRGRILAAFMFALPL
                     SLAVGAPLSTALLGLDGVFSLRGWQWMFLIEGLPPVLLTVVALKLLKNSPDEAHWLSP
                     DERRWLAAELARERAAIHMPNVSPLRIALVSPIVLVLAFVYFCATSANLGLSFFLPQM
                     IREQGYSIVESGWITSLTYVAGAVGMIGFGYLSDRLRGHYGCLVAPLLIAVAGLVGTA
                     WLDDSVWGIVAMSVAAIGILGFKSPFWGVPSGFLSGPAAAVAIASINSIGNLGGFAGP
                     FLLGAVRQTSGDYRVGLYVLAVGLLVSAFAVRGPARRPRQSFLAR"
BASE COUNT         2574 a         4190 c         4029 g         2447 t
ORIGIN      
        1 atcgacatgg tgtgcacgaa ctcgacttcg gattcgtcga ccgcgccgga gatcggcgcc
       61 gcgctgccgg gttcgggcgt gcaggcgacc ggaatgtgcc cttccccggc gagcaagccg
      121 gaggtcggat cgaggccgag ccagcccgcg cccggcaggt agacctcgca ccatgcgtgc
      181 aggtcggtga agtcgacttc ggtgccgctc gggccgtcga tggatttgac gtcgggcgcc
      241 agttgcaaca gataaccgga gacaaagcgc gccgccagcc ccagttgccg gagcgtctcg
      301 accagtagcc agcccgaatc gcggcacgag ccggccgcgc tcatcagcgt ttcctcgggc
      361 gtctgcacgc cgggctccat gcggatcaga tagcggatct cgcgttgcag ccgctgattc
      421 agcccgacca gaaaatcggc ggtgcccatc ggcgtgcggt cgatactcgc gacgaacccg
      481 gcaaagcgcg gcgtcattgg gcgcttgacg cgatacggcg cgagttccag tgcaaggccg
      541 ggcgcatact cgaacggaaa ctgttccgcc gacggttcga gaaagaagtc gaatgggtta
      601 tagacggcca tctcggcgac cagatccacg gtcaccttga attcgcgcgt cttctcggga
      661 aacaccagac gtgcctgata attggcgaac gcgtcctgct gccagttgat gaagtgcccg
      721 gccggttcga cccgcatcga ataagacaga atcggagtcc ggcaatgcgg cgcaggcctg
      781 agacgcacga cctggggcga caacgcaacc agcctgtcgt agcggtaatg tgtgacatga
      841 ttcaacgcga cgcgtatgga cacaccgaac tcctggacaa aggatggcag cccgcccaaa
      901 aaagcaagct tcatgccgtt agagaacgaa cgcgcaaagc cgcgtgaact gcctgtaccg
      961 cgcggattag cgcctgctgc caggtttgcc gcatctgccg gccgccggcc cgatcgatcc
     1021 gtgcgccgtt tcgccgccgt ttcggcggcc gcgcaccaaa gcggcgcagc agcgatgcac
     1081 gatcatgcac gtactgtgcg ttaaaccagt ggcgtctggc aatcgcggca tgaagattgc
     1141 gcgtcgtctt cgctcttgca ggcgcttgag gatgctcgaa atgcccggtg gccagcatcc
     1201 ggccgctgac aacgcacctc gcctgctgct ccacgactgc tacccattac tcgggctcga
     1261 tcgatgaaaa ccttccactg caatcgctgc tcgcacctcg tgttctacga aaatgttcgc
     1321 tgtgaacgct gtgagtcgtt gctcggatat gtgcctgaac tcgccgaaat cagcgccttc
     1381 gaggaagccg atgaaggacg ctggcgcagt ctgcatccgc gtgccgaggg cgcgctgttc
     1441 cggcaatgcc acaactacgc ggtcgaaaat gtctgcaact ggatgatccc cgccgattcg
     1501 cccgacacgt tgtgccgcgc ctgccagctc acgcgcacga ttccaaatct gagcgcgccg
     1561 gacaaccggc tctactggta ccggctggag atggccaagc ggcgcctgct gtacacgctg
     1621 gccgcgctcg gcctcgacgt gccgtcgcgc cagaccgatc ccgagcacgg cctcgctttc
     1681 gagttcctcg aagacggtgg cgatggttcg cgggtcatga ccggccacga caatggcctc
     1741 atcacgctga acatcgccga agccgacgac gtctaccgcg agaaagtgcg cagcgcgatg
     1801 ggcgaaccct atcgtacgct gctcggtcac tttcgccatg agacgggtca ctactacctc
     1861 agtcaactgg tcgaaaacaa tccgcgctgg atcgagccct accgcaagct gttcggcgac
     1921 gagcgcgcct cctatggcga ggcgctaacg gcctactatc gcgacggcgc accggcggac
     1981 tggcaggcct cgtatgtcag cgcctacgcg acgatgcatc cctgggaaga ttgggctgag
     2041 acgtgggctc actacatgct cattgtcgac gtgctggata cctcgacctc gtacggcctc
     2101 gccttgttgc ctgacgaccc gaacgagccg acgctcaccg accgcacgcc ggtggaagat
     2161 gcgagcttcg agaatctgat gaagcgctgg tttccgctca cgtatgcgtt gaacagtctg
     2221 aaccgcagtc tcggcatgcc cgatggctat ccgttcacgc tggcctcgcc ggtggtggac
     2281 aagttgcgct tcgtgcatcg ggtgattgcg gcggcgggcg acaaaaccca gccgcgggaa
     2341 gcggcgaagc cggcgaatgc tgcggggaca gtgcagacga cggcagccgg cacatcgccg
     2401 acggctgggg cgccggcaac cactcaacct gccgcgcagc cggtggcgca gcagcgggtg
     2461 acgcaagccg tgaggtcgca agaaccgtcg caaacggcgg atcagacagc gccgccacaa
     2521 ccgacacagc ctgtcgctca agcagcagtg ccgcaagatt gcgcgcaggc gccagccatg
     2581 caagcggtcg agccatccgc gccgccgttg caatccgaat ccagcgccgt gaaaacagca
     2641 ccgcccaagc ggaccgcgcg gcgacgttag tccggttgct gtgttcggtt gctgtgttcg
     2701 gttgctgtgt ctggtcgctg tgtctggttg ctgtgcctgg tcgctgtgcc tggtcgctgt
     2761 gtctggttgc tgggtctggt tgctgggtct ggttacggtg cccgtaagtg acacgcttcg
     2821 cgaacccaca aagaaaaccc gctgccgact aaaccggcag cgggttttct ccacctgagc
     2881 caagcctgac tatcgctcaa tacgcgacgt aaggccccgt accacccggc gtcggctgct
     2941 gctcgacggc ggcgtacacg ttacggccgt agaagaacgg cagaccccag tcgaaaatcg
     3001 acgacgtcac aaaagccggt cccgccagca acggcagcgc cgaatcgccg ctgtacgtct
     3061 gcgagatcgt cgtcgcgttg ccaaccgcga agttcaccgt actcgtcatg ccgttcaccc
     3121 cttcgatcat cgcactcaac gattcaatag ataccggaca gtagtacgcg ctattagggc
     3181 tcgcgcatac cggcaacgca ctggtctgaa agaacaggcc ggtcgagccg ctgtccagaa
     3241 tactgttcga atacgacgta ccgttctgca ccgtcgtaaa ggtgccgttc gacggactga
     3301 cacccaccac ctgcgcgctg ccgagaccat tgttgccctg cgtgccgatg ccgaacacca
     3361 gttgcccggt gaccgactgc gcgccgcccg acaccaccgg caggctcagc atggagccgt
     3421 tgttgtcggt ggtgaagtac ggaaccggat tggcgacctg gagcgcctcg gcgagcggaa
     3481 tcgaggtgca tgccgcgcca ttgcaaccgt agtacgtgcc ggccaccgcc tgctgcacac
     3541 agttcgcgcc gcagtcgtgc ttgaacacgc cgatgcccag aatgccattg gcctgcaacg
     3601 tccccggcgt gttgcgcgac gcgccgtagg tcgcgcaatc ggagggcagc gaagcatagt
     3661 tcggatcgat tacctggatc ggcagcgagg ccgctttttc accggcgatc tgcaggtcgg
     3721 cgagtttcac cgaaccccac gtgtagccgt cgaagaactg catgcattcg gcaaccagat
     3781 tgcccgagcc gtccttctgt tgcggcagcg tcatcgacgc gggcaattgc gaggcgaaca
     3841 cgcgcacgcc ccacgagccg gtatccacga ggatgtgatc gatggtttgg cactgcgtgg
     3901 cgcccggcgc gcaaatcgtg atgctgacga acggcatgtt cggcacattg ctcacgccgg
     3961 agtcgactgt cacgcgcact gcattggccg ccaacacttg cggcgaaggc gcggtattgg
     4021 tgacagtggg accgggcgaa acgctcgtcg tgttccccga attcgccgcg gagccggagc
     4081 tgctggagcc gccgccgccg ccaccgcagg cactgacgag cagcgcgagc agtagcgcgc
     4141 acaacaggtt aatgaacgaa gcgctatcac gcatgatccg ctccattatt gaatgacgcc
     4201 gacgtcggcg cccgcgggca ccgcttgcgg cagatacgca tagccggcaa acgcgcgcag
     4261 gtgaccgccg gaataaacga ccagatcgcg gctcgacacg gcgagcggcg tgccgcggcg
     4321 cgtcgcgatg gcggcgacat attggtcgaa cgacgcgccc agcacggctt tcagattcgg
     4381 cagcgtcggg ccttgccacg cgacaccgaa caccatgccg ttgggcgcga cgtactcgcg
     4441 caccagcgtg ccggacggca tggtcattag atgtaccgaa tacgcgtttt gcgtagtggc
     4501 cgtacgagcg actgcgcgaa ggcgcgtctg atcgctgtcg acagtgttga cgttctggcc
     4561 gagcgtggcg tgtgcggcaa acgacagtgc gcagcttgcc ccgagcattg cgcgagcaat
     4621 gctggtaaag agcatcggtt tcaagagatt tccttcgagt tgaaacgctc ctgcccggcg
     4681 ggcgagccga gcacgaacgg gaatccaggg gcgcacgcct gaccggcggt gccggattgc
     4741 attcacaccg tccgggttgc gtgaatgcgt ctacggagtg gcggagcgaa actttaggca
     4801 cggcgcaacg ccatggcaat cgtttgcggg atgatttaaa ccttgtgcga atgccgcgcc
     4861 gttttatgcc gtttgatatt tgaggacaga atcaccaagg ggcggcgttt cgccgcatat
     4921 tttcccgatc agagatgttt aaagcgaacc cacgcaaaaa gtaacgacgc cacgccagaa
     4981 atttatcgag gcgtgccggc atccggcgag tggtgtaagc gtgcgctgaa taatccgcgt
     5041 ctgccggcct atttcatctg ataattgcgg cgcatacaga ctgcttttgt atgaagagaa
     5101 ttcgcgatct caatcgtgat tcgaggcaaa aaaaatgccg cggataacgt aatcgtcgtc
     5161 cgcggcaccg gatacttcct ggcgagcttc tcgctgtttc atagcaactt ccgcaagtcg
     5221 cgccgaattc gcatcaagcg attcgacaag gcccacagtc cttacttttt ctgttgctgt
     5281 tgcaagagcg atttgagctc ctgcacattt tcttctacgc gttttgcgat caccgcatac
     5341 gactcggcct gtgtctgata cgcggcttgc gcgagttgtt gcatatcggc gagcgacttg
     5401 tgcagactct gctggatcag ttcggcggct ttcgccggag gcgctgcgcc gcccgccgac
     5461 aattgcccgg tgagcgactg caattcggcc agcgtggcac gcaacatctc ggtctgcttc
     5521 tgagcgagcg actgcatgcc ctggaatgcg gtctgattcg cctgtaccag tgcctccatg
     5581 tccttgcgtc gcgcttccat gatggcgggt acgtcgaagc cggggagctt gaactgctcc
     5641 agcattttgg tgaaatcgcc aaacggattg gcggcgtcag gttggctcat gcgaaatcct
     5701 ccttaacggt tgaatactcg ccggcggtgc atcgccgcga tgggctgctt cgacctctgc
     5761 accagtggcg tcgcgtgcgc cgcgcgcctc ccgcatagac ttgctgcttc tggggtcgga
     5821 cggcacatcg tttccatcat gcgccatccg ggcacgttgc gccagcgggc acaccctggc
     5881 gtcgagcgtc ttcttgccgg atcgcttacg gcttcggcca cccctgtgtc gcgccgtctt
     5941 ctccgcagtt tttctgcaac ggtgctcctt ctagcggcgc cgtgacgcat gcaaggtttg
     6001 gtaagttaca aggcgacttc agtaacccca ccaaacggcg tgtcgactat cgttgcccat
     6061 gtgccggcgc actgtcgcgc cggcgctgtc cgatgagagc gagatcatga aaaaaggatt
     6121 agtgagcgcg gcactggccg ctgttttgac cgtggcggcg actggcgcgt gggcgcagga
     6181 agctgcaccg tcgcctgctt cagcccacgc gcgcggcgag ccgcggcgcg gcgacgaagc
     6241 gcatcaccca cacgggccga tgggcccccg tttcgcggtg atccacgacc tcaaacagtt
     6301 acgccgcctg tatgcaatga gcggacgcga aggcgaaatc gtcgccgtgt atcacgaggt
     6361 gttgaacaag acgcaagatc cgatgctgcg ccactacgtg tacgacgcgt tggcgcgcga
     6421 gcagttgaag ccggccaata ccgaccaggc catcgccacg ctgcgcaccg gtttgtcgga
     6481 agaccttgtc gcggccacca agccgcgcgg catgccggcg gggatgcaac cgcctgcgcc
     6541 gccgcagtag gactttgccg tacggggttg gggcgaagcc accctgcgaa ggcacgccgt
     6601 gaaggtctgc ggtcgcaaag tcagttggaa gcgctcccgc cgaaacgcag cgcggccgtt
     6661 cggccgcgct gcgttagctt tcgcacatag ccgcctcact gcccgctttt catcttctgt
     6721 caggtgatta ggataagagc accgaaacgc ttgtgcctgg tgaaccatgc gctacccgct
     6781 gaacatggcc gtcgccgccg cccttctcgt gttcgttctt tgctgcatca tcaagctcgc
     6841 gttgaattga tgcctcgaat tcacatccag tcgcacgttt ttcatgctct caccgacttc
     6901 gtgtagcgca gccagaccgc gtcgttcccg gtcttttcaa cggactcaag cttcagatat
     6961 gccggcgctt gccatttgtc catagctact tcgaacgaag acggatgctc gctgcggcca
     7021 tccaccagcg gcaagatcaa cacgctgact tcgtccgcga gtcccgcgtt cacaaacgcg
     7081 ccgctcacat gcgaaccacc ttccacaatg agctttttca cgccgagttc gcgcgcgagc
     7141 gtctgcacta ctttgttgag atcgatttca gattttccgc cgaacacata ggagacgccg
     7201 atcgattgaa gataggccag atagtcgtcc gcaacctgct cggtcagcac ttcgacgatg
     7261 tgcgacttga gcgcggtgtt gctcttccac gcaacccggc ctcgggggtc gatcgaaatc
     7321 gcgtactgct gcgcgttccg ctcgacgaaa tgatcggtgc gcggcacggc gcctttcgcg
     7381 agccctttcg ggtaatccgt gccgtgcgaa atttcctgca tggtgacgcg accgcaaatc
     7441 cagccgtcgg ccttgaaagt cgccgcggtg ttttcgtaca gatcgctggc aaagtcgaga
     7501 tgccagccgt ccgtcagact gcggccgtct agcgaggaca tcatgtgaac gacgatgaac
     7561 ggcttcatgc gttaaggctc cgtgtaagtg aatacacgga gccttcagca aggtctgttc
     7621 cgcaatctac ggattggcgg agcgcttaag cgacagcgta acgttcgagc caatgcgcgt
     7681 acggcgccgg cagcgtccac gacggacgtt cgataccgag cttgcgcgcc gcgtgatacg
     7741 gccagtgcgg atcggccaga tgcgcgcggc cgaccatcac caggtccaat tgctcgttct
     7801 tgacggagcg ctcggccaat tccggggtat cgatgcccca tgccgacgag acggcgatgc
     7861 cggcctcgcg gcgcacgcgc tcggcgatcg gtgcgaggaa tgccggcgcc cacggaatag
     7921 ccgcctccgg cgtggagaag cccacgctga cgctcagcat gtccaggccc tcgcgcttga
     7981 agttcttcgc cagttcgatc gattcggcga gcgtttcctc atcgcgcccg tcgtattcga
     8041 tcacgccgaa acgcgccgtt aacggcagat gctcaggcca caccttgcgc accgcagcca
     8101 aggtttccag caggaagcgg ctgcgatttt cgaggctgcc accgtatgcg tcgtcgcgtt
     8161 gattggagtg cgccgagaag aagctttgac cgaggtagcc gtgtgcgaag tgcagttcga
     8221 gccattcgaa acccgcgtca cgggcacgcc tggcggcggc gacgaaatct tcgcgcacac
     8281 gcgcgatgtc gtcgagcgtc attgctttcg gcactttcgg cagcccgcca ccgaaggcga
     8341 tcgccgacgg cgcaagggtc tgccagccgc gcggatcggt gtcggcgatg tggtcgtcgc
     8401 cttcccacgg gcggttggcg ctcgccttgc ggcccgcgtg gccgatctga atgcccggca
     8461 cggcgcccgc cgccttgatc gacgcgacca ccggtgcgaa ggcctgcgcc tgctcgtcgg
     8521 tccagaggcc cgcgcagccc ggcgtaatac ggccctcggg cgacacagcc gtcgcctcga
     8581 cgatcaccag ccccgcgccg ccgcgcgcca atccggcgag gtgcacgcga tgccagtcgt
     8641 tgatcaggcc gtcttcagcg gtgtactggc acatcggcgg aatcgcgatg cggttgcgta
     8701 aggaaacatc cttaagcttg tagggctgaa atagtgcgga catcaggcta aactccaatg
     8761 gataggcttg tacggtagtt cgataatgat cgaactaccg agtatggcag aaacccttat
     8821 cacctgcaca atgcgcacct atacccatcc tgccgctgag gactacacgc ttggacggct
     8881 tttccatgcg cttagcgacc ccgtgcgcct cgagatcgtc cgtcgccttt caacgatcga
     8941 cgaggccacc tgcggcgacc tcgacggcgg ccgccccaag tcgagcgtct cccatcactt
     9001 ccggatcctg cgtgaagcgg gattggtgcg tacgcgcgtc gccggcaccg tgcatcagaa
     9061 ttcgctacgg cgcgctgaac tggatgcccg gtttcccggc ttgatggagg cgatcgtcaa
     9121 gcagatcgtc aatgagccgg cgttgaccga accggcttga tggtcgattg aagatcgacc
     9181 gaggatcggt ggcgcggcgc tacgcacatc acaactcaac tcaccgttct actcgcgcca
     9241 gagtatgcgc atcagcgcga gcaacgcagt cggcgccgtg cctttctgac aatagccgtc
     9301 gaaattatga cgcgccttgc cggtttcttt tacgtgatct tcatcgagcg aagtaaaggc
     9361 gacgatgccc aggctctgcg tcggaagata gctgcgaagc gcgctcgcgg tttcgtagcc
     9421 gtcgcgctcc ggcatcatga tgtcgagcac gacgatgtcc ggcacccaat ccttcacgca
     9481 gcgcagcgcc tcgctgcaac cgttggcggt acgggcttcc acgccttcat acgagagata
     9541 ggtggcgagc gcttcggcgg cgttggcgtt atcgtcgacg acaagtacgc gcggcacttg
     9601 gtgcaatatc cggacttcgc gggcgctcca cctttgggaa atagagggtg ttcgggacat
     9661 gtgtggcagc ttgcgataaa aacacgagtc gtcagcaaac tgcgtaccac ggtgatctga
     9721 aagtgcccga catccgtgcc atgctcgaca cccacgccgg gtcgcactta tcgggcgtcg
     9781 ccagtggaac tgaagcgccg ttggcggcgg ctggatgtaa tctgtccgtc acccgccggc
     9841 aatatggcgg tgtaatgatg atgcttcgta cccgcccctg tggctcgaga cattgcagcc
     9901 ggatgcgctg caatccgaca ccgcccgggc gggcacgact cccgccagat cttgcacccg
     9961 atgatgatgc ccgcttgatc cttcacggga cgtctcgtct tcaccgtctc gagcggaaac
    10021 acacgcggta gttctaccgt atgcaatggt gttcaatccg ccggcagcgg cacaggctgc
    10081 tgagcggctc tgcgctgcgt tagacacccg tgtccggtaa catctccaca tgaacctgga
    10141 cggcggcctt gagccaccct cgtcgccagc ggcgctcggt ctcagcgaaa ccgatctcgc
    10201 ttacgcggcc gaagtcgcca cacgctcgcc aaatgtcaat ccgaggccgc tgtcttgcgg
    10261 cgcggttcgc cgcttgctgc aaatcgcttt cgacggcgca ccgccgagtc tgcattcatt
    10321 cgacgctgtc taacgaattt cgctggagac gcgttccatc cacggggaat gatcggtcgc
    10381 agaaccctcc cctgtgtcaa caggcattca ctttttgaat ttctcactca tggaaaatcg
    10441 ggcacttatc atcggggcta gcggcattgt cggcaacaat ctggccgatc gtctgcttgc
    10501 cactggctgg agcgtttcag ggctctcgcg cggccgcact gcggtgtcgc ctgcgatcga
    10561 atcgatcacc gccgatctga cctcacctga atcagtcagc cacgcgctcg ccggtagagc
    10621 cttcagtcac ctgttcttca ccgcatgggc ccgccaggcc acggagaagg acaacatccg
    10681 cgtcaacggc ggcatggtgc gtcatgtgct ggacacactt ggaccggcag gcggcgtgtc
    10741 acacgtcgcg ctcgtcaccg gcctgaagca ctatctcggt cctttcgagg cgtacgcgac
    10801 gggcgcggtg ccggttacac cgtttcggga agaacagggc cgtcagccgg tcgacaattt
    10861 ttattacgaa caggaagacc gcctgttcga agcggcgcaa cgatacggct ttagctggag
    10921 cgtgcatcgt ccgcatacga tcatcggctt tgctgtcggc aacgcgatga acatgggggt
    10981 gacgctcgcc gtctacgcaa cgctttgcaa gcaggagggc aagccattcc tgtttcccgg
    11041 ttccgccgcg cagtgggaca gtctcaccga catgacggat gcccggttgc tcgcgcgaca
    11101 tctggaatgg gccgcgactt ccgcgaatgc ccgtaacgag gatttcaacg tggtcaacgg
    11161 cgatgtgttt cgctggaaat ggatgtggtc gcgcattgcg gattacttcg gcattgaagc
    11221 gattccattc gatggcgtgg tgcgtccgct cgagagccgg atgcaggacg cccaacagca
    11281 atggtccggg attgcggcgc ggttcggtct gatcgaagcg gacatcggca agcttgtgtc
    11341 gtggtggcat accgatgccg atctcggacg cccgatggaa gtgctcaccg acatgacgaa
    11401 gagccgcaag gcaggctttc ttgactatca gagcacgcca gacgcattct ttgcattgtt
    11461 tgaccggctg aaggcggagc ggatcattcc tgcatgatgc acccgaaacg aagggcgctc
    11521 attaccttct gcaaacgcgg gtagagctac ttgacgggcg actgcttgcg ctcgagtgaa
    11581 acccgcaacc agttgccctt tagcgagcta aaaaagattg ccgcggtcga cgcgcgggcc
    11641 ccctcaccgc aaaggcggag accagcagcc cgactgccag tacatacagg ccgacacgat
    11701 agtcgccgga cgtctggcgc accgcgccca acaggaacgg cccggcgaag ccgccgagat
    11761 tgccgatcga gttgatcgat gcaatcgcca cggcagcagc cggtccgctc agaaagccgg
    11821 acggcacgcc ccagaacggt gacttgaagc cgagaatgcc gatggcagcg acgctcatcg
    11881 ccacgatacc ccacacggaa tcgtcgagcc atgccgtacc gaccaacccg gcaacagcga
    11941 tcaacagcgg cgcgacgagg cacccgtagt ggccgcgcaa ccggtccgaa agatagccga
    12001 agccgatcat gccgaccgcc ccggccacat acgttagcga cgtgatccag cccgactcca
    12061 cgatcgaata gccctgctcg cggatcatct gcggtagaaa gaacgacagc ccgagattgg
    12121 ccgacgtcgc acagaaataa acgaacgcga gcaccagcac gatcggactc accaacgcga
    12181 tccgcagcgg actcacgttc ggcatgtgga tcgcagcccg ttcgcgcgcg agttcggcgg
    12241 cgagccacct gcgctcatcg ggtgacagcc agtgcgcctc gtcgggagaa tttttcagca
    12301 gcttcaacgc aacgacggtg agcagcaccg gcggcaaccc ttcgatcaga aacatccact
    12361 gccagccacg caacgaaaag acaccatcga gtcccagcag cgcagtcgac agtggcgcgc
    12421 cgaccgcgag ggataacggc agcgcaaaca tgaacgcggc gagaatgcgc ccgcgataac
    12481 gtgccgggaa ccagtcgttg aggtaaaaaa tgatgccggg aaagaaaccc gcttcagccg
    12541 cgccaagcag aaagcgcagc accagaaaac tcttctcgcc ggcgacgaac gccgtcgcac
    12601 aggacagcag tccccacgtc agcaggatgc gcgcaatcca gcggcgtgcg ccgaagcgca
    12661 ccatcaacag gttgctcggc agctcgaaca gaaaatagcc gatgaagaaa atgcccgccg
    12721 cgagaccgaa cacgttgctc gcgatcccga gatcgccgtt catcgtcagc gcggcgaaac
    12781 cgatgttgat gcggtccacg taggacacga tatacagcag cacgacgatt ggaacgatac
    12841 gcaccgccgt cttgcggatg gcgctgcgtt cgacatcatg tgcggcgatc gttttcaatc
    12901 cggacgtgct catggtggtc tcctcgcagc ccgctttctc atgcacggcg aacccgcgcg
    12961 accagagagc gtgtagttgt tggcgttggc gttgggctag cggctatcgg ttgccgggaa
    13021 gcgcgatacc gggcgtcgtc cagtcgaacg acagcaatca ggcgcctgcg ctgcgacagc
    13081 atgatctgtc tcctgcgcga ctcttcgatc gcgaagatag ttatccgcgg gccggcgcct
    13141 gcccacttca aaatgggcgc cggtcagcga ctgcatgcct cgtcatcctg ttcgttgcgc
    13201 cggccagagc cggtttcacg cggcgctcgc cgtgacgacc
//
LOCUS       DATASH010000445         1533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2247144,
            whole genome shotgun sequence.
ACCESSION   DATASH010000445 DATASH010000000
VERSION     DATASH010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2247144"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1533)
                     /locus_tag="VHV99_12285"
     CDS             complement(<1..>1533)
                     /locus_tag="VHV99_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636248.1"
                     /translation="NINGVDTDPAFWGPPALGFNSGLYGLSDGPNSNTRNLTQSINVE
                     VQWNRSRHNVAVGGDFRRQEFNYRDQSNARGTFTFNASATNGGVTTAGSDLADMLLGI
                     PDTSKIAFGNPDKYLRQSVYDLYARDDFRVNPEFSINAGVRWEYGAPITETKGRLANL
                     NVGSGFSSVSPVVGSNPTAGFPSSLVRPDKSGIAPNVGIAWRPISGSSLLIRAGYQIS
                     HDTSVYQSSAYSMATQAFATPLTTSASLNNATCLLSLTQGFPSCPSITSDTFAIDPNF
                     RVGYVQIWQLSAQRDLPASLQAVVTYRGTKGTRGVQEFLPNTCPYSSVSTACTSAPTG
                     YVFRTSNGNSTREEGIATLRRRLRSGFTANLTYTFSKSIDDDYSFGGGTGTGVNPQVA
                     QDWLHPERQRGLSTFDQRHLLAAQVQYTTGMGIGGRSLMSGWKGALYKEWTLLTSITA
                     GSGLPETPLDPVAVPGTGYSGIIRAHYTGAPVYVTSPGRFLNPAAFTAPADGQWGDAR
                     RNS"
BASE COUNT          266 a          501 c          485 g          281 t
ORIGIN      
        1 cgagttgcgc cgcgcatcgc cccactgccc atccgcaggc gccgtgaacg cagcaggatt
       61 caggaagcgt cccggcgagg ttacgtagac cggtgccccg gtgtaatgcg cgcggatgat
      121 gcccgaatat ccggtgcccg gcaccgcgac cggatccaac ggcgtctccg gcagcccgct
      181 gcccgcggtg atgctggtca gaagtgtcca ctccttatat agcgcgccct tccagccgct
      241 catcagcgag cgtccgccga tgcccatgcc ggtggtgtac tgcacctgcg ccgccagcag
      301 atgccgctgg tcgaacgtcg acagcccgcg ctgcctctcc ggatgcagcc agtcctgcgc
      361 cacctgcgga ttgacgcccg tgcccgtacc gccgccgaag gagtaatcat cgtcgatcga
      421 cttcgagaag gtatacgtca ggttcgcggt gaagccgctg cgcagcctgc gccgcaaggt
      481 cgcgattcct tcctcgcgcg tcgagttgcc attcgacgtg cggaagacat atcccgtcgg
      541 cgccgatgtg cacgccgtgc tcaccgagga gtatgggcag gtattcggca gaaactcctg
      601 caccccgcgc gttcccttcg taccgcgata ggtcaccacc gcctgcagcg aggccggaag
      661 atcgcgctgc gcggagagtt gccagatctg cacgtagccc acgcggaagt tgggatcgat
      721 tgcaaacgtg tccgatgtga tcgacgggca gctcgggaag ccctgcgtca gcgatagcag
      781 gcaagtcgca ttgttgaggc tcgcgctggt cgtaagcggt gtagcgaagg cctgcgtcgc
      841 catggaatac gcggagctct gatacaccga ggtgtcgtgg ctgatctggt agcccgcacg
      901 aatcagcagc gacgatcccg agatcggccg ccacgcgatg ccgacgttcg gtgcgatgcc
      961 gctcttgtcc gggcgcacca gcgaactggg gaagccggcc gtcggattgc ttcccaccac
     1021 tggcgacacg ctcgagaatc cggatccgac gttgagattc gccagccgcc ccttggtctc
     1081 cgtgatcggc gcgccgtact cccagcgcac gcccgcgttg atgctgaact cggggtttac
     1141 ccggaagtca tcgcgcgcgt acaggtcata caccgattgc cgcagatact tgtccggatt
     1201 gccaaaggcg atcttgctgg tatcgggaat tcccagcagc atgtccgcca ggtccgagcc
     1261 cgccgtggtc acgccgccat tggtcgcgga ggcgttgaag gtgaacgtcc cacgcgcatt
     1321 cgactggtcg cggtagttga attcctgtcg gcggaaatcg ccgcccaccg caacgttgtg
     1381 ccgcgaacgg ttccactgca cctcaacgtt gatcgattgc gtcaggttgc gcgtgttgga
     1441 gttaggcccg tcggatagac cgtagaggcc gctgttgaag cctagcgccg gaggccccca
     1501 gaacgccggg tctgtatcca caccgttgat gtt
//
LOCUS       DATASH010000446         3574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2048530,
            whole genome shotgun sequence.
ACCESSION   DATASH010000446 DATASH010000000
VERSION     DATASH010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2048530"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..360)
                     /locus_tag="VHV99_12290"
     CDS             complement(172..360)
                     /locus_tag="VHV99_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636249.1"
                     /translation="MTKQKHQAPQDKQAAQAAQSAESRLDEALEESFPASDPIAVDMT
                     EPSHSPPEAEPSEAKKPH"
     gene            724..951
                     /locus_tag="VHV99_12295"
     CDS             724..951
                     /locus_tag="VHV99_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636250.1"
                     /translation="MDQKAPARAQRFTASHVVEAELDHVDWATRQPALRMLDAVYWRR
                     RVLAVKCGFELRDLQVMRLEKILQRLGHPSE"
     gene            complement(972..1487)
                     /gene="hfq"
                     /locus_tag="VHV99_12300"
     CDS             complement(972..1487)
                     /gene="hfq"
                     /locus_tag="VHV99_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEX3636251.1"
                     /translation="MASAESHPQNDFMNAARKERKRVEIYLVNGIRLTGCIESFDQYL
                     VMLRTPVGLQGIYKRAISTIQLDTGTRPAPRAGRPSHGEHTTRGPHGSREPRDHRDPR
                     EPRESYGAPASDRPASDRSGSTSDGPVVVTRRRRLFGTGGGDGGNHGGGNHGGNGGTS
                     GGNGGTSGGNE"
     gene            1841..1984
                     /locus_tag="VHV99_12305"
     CDS             1841..1984
                     /locus_tag="VHV99_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636252.1"
                     /translation="MKVSRILSAAFVLLALALGSFAMSGCTSGTDSGASSTSGGSSGG
                     GGY"
     gene            2000..2542
                     /locus_tag="VHV99_12310"
     CDS             2000..2542
                     /locus_tag="VHV99_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3636253.1"
                     /translation="MNFEAEVISWLPQLRRYARALTGDRAWADDLVQDTAERALARWA
                     AFRPNSNLRAWLLTILRHLYIDQLRGRREIAVDEESAPWRNLEAPHGEVDGLMLRDLQ
                     RALYCLPVEQREVLLLICVEELSYQEASKALGVPIGTVMSRLSRAREHMRVLMTEGPV
                     EGGAGGLARKVPPLKVVRNP"
     gene            2542..3387
                     /locus_tag="VHV99_12315"
     CDS             2542..3387
                     /locus_tag="VHV99_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX3636254.1"
                     /translation="MNNDHDTSLPEGLDLRVLSAFVDDELTPAERAAIETQLAQQPQA
                     AARVAAWRAQKAGLQALCGAPLRNERGGGAGQSEADEPAFIVVRRPAPSWQRMGLAAC
                     WLALGAGLALALGPLAPRLTGGAWNGLGGQPPSFAERADIAYAVYTPEQRHPVEVAAS
                     EEEHLINWLSKRLNRPLSVPSLQEYGYSLVGGRLLPGEAGPAAQFMYENRSGARLTLY
                     ITGISRDETAFRLFRDGNRRTFYWVSDRMGYALSGPIAEGKLRSIAVDVCSALGGKPE
                     TWQER"
     gene            3372..>3574
                     /locus_tag="VHV99_12320"
     CDS             3372..>3574
                     /locus_tag="VHV99_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636255.1"
                     /translation="MAGALTARRVGQARITLEALEALEAAEAAEAAEAAEAAEAAEAA
                     EAAEAAEAAEAAEAAEAAEAAEAL"
BASE COUNT          711 a         1093 c         1183 g          587 t
ORIGIN      
        1 tgagccgctt tcttttgcct acttttcttt gcggccggca aagaaaagta ggtgccgccc
       61 cgcacagggg caacgctaat agaccacaaa gaaaacaagt aaaggccaac gccacgggca
      121 aacagcccaa aaaaaaatca ccacacgacg caaaaccccc agccccaaaa cttaatgcgg
      181 cttcttcgcc tctgaaggtt ccgcttctgg tggcgagtgg gagggttcag tcatatcgac
      241 ggcgatggga tcactggcgg gaaagctttc ttcgagagcc tcatcgagcc ggctttctgc
      301 ggactgggcg gcctgcgccg cttgtttatc ctgaggtgcc tgatgctttt gcttggtcat
      361 gacatgctcc gttagaagac agctgacagt ccagcatagc gcaatggggg ccgacctgag
      421 catgggccga acggcttggg ctctcacgcc gtaagcattt ccttcagagc caaaacccaa
      481 actgcaaaac cccaaccccc aaccaaaaat gcagaccaga aacccatcaa caaaaaacaa
      541 aaattaaaac caccccgcat gaaatcgtct tcggagcgtc gccagaaaaa aataaacagc
      601 atgcgcagat ctgcagcccc actggcaaag cccacaggaa gccgtgaacc ggcaacgtcc
      661 agccctggag aggagaggac cgaacaaaac aaaaccaccg aagaacaaca aggcaccacc
      721 gacatggacc aaaaagcccc tgcccgcgcg cagcgcttca ccgcctctca cgtagtggaa
      781 gccgaactgg atcatgtgga ttgggcgaca cggcaaccag cgctgcgcat gctggacgcc
      841 gtgtactggc ggcgccgcgt gctggcggtc aaatgtggat tcgagctgag ggacttgcag
      901 gtgatgcgac tggagaagat tttgcagcga ctcggccacc cgtcggagta gccggccgct
      961 ggggcggcag tttattcgtt gccgccgctg gtgccaccgt tcccaccgct ggtgccacca
     1021 ttgccaccat gattgccgcc accatggttg ccaccgtcgc caccacccgt gccgaacaga
     1081 cgccgacggc gcgtgacgac caccggaccg tcggacgtgc tgccgctacg atcggacgca
     1141 gggcgatccg aggccggcgc gccatacgac tcgcgcggtt cgcgcgggtc acggtggtcg
     1201 cgcggttcac gcgaaccgtg cgggccacgc gtggtgtgct caccgtgcga aggccgcccg
     1261 gcgcgcggcg ccggacgtgt gccggtgtcg agctggatcg tcgagatcgc gcgcttgtag
     1321 atgccttgca gcccgacagg cgtgcgcagc atcaccagat actgatcgaa cgactcgatg
     1381 caccctgtca ggcgaatgcc gttgacaagg tagatctcga cgcgctttcg ttctttgcgc
     1441 gcagcgttca tgaagtcgtt ttgcggatgc gattctgcgg aagccatgga caattcaggt
     1501 taggaagacg gtggttcgcc gcgattctac cctgtgtgga ggtggaacgc gtcagcggac
     1561 aaactgcgtc cactataacg gcttgcgcgg acataagcat ctgataactt ttgactgtaa
     1621 cgttgagtta cgaatctgcg gacgaattgc gctttcttcg gccacgttcg ggcgcgcccg
     1681 gtgggaatgg cggaaaaaag atagcgcata ccggcgcgtg cgtggaataa atcccggcct
     1741 gcccacgtta agtcagacat ggcagcgctg catatcgcac gtggccggtc ggccctcccg
     1801 gtccgcatga acgggccggg cgctcatcag gagactcacc atgaaagtat cccgcattct
     1861 ttccgctgcg ttcgtgctgc tggcgctcgc gctgggctcg ttcgcaatga gcggctgcac
     1921 ctccgggacc gacagcggcg cgtcgagcac cagcggtggc agcagcggcg gcggcggcta
     1981 ctgagcgttg ccacgccggg tgaatttcga agcggaagtc atttcgtggc tcccgcagtt
     2041 gcgccgctat gcgcgcgcac tgacgggcga ccgcgcctgg gccgacgatc tcgtgcagga
     2101 tacggcggag cgcgcgctcg cgcgttgggc ggcgtttcgt ccgaacagca acctgcgtgc
     2161 gtggctcctc acgatcctgc ggcatcttta catcgatcag ttgcgcggcc gccgcgagat
     2221 cgccgtcgac gaggaaagcg cgccgtggcg caatctcgaa gcgccgcacg gcgaggtcga
     2281 cggtctgatg ctgcgcgatc ttcagcgcgc gctgtattgt ctgccggtcg agcaacggga
     2341 agtactgctg ctcatttgcg tggaggagtt gtcttatcag gaggcatcga aagcgctcgg
     2401 cgtgccgatc ggcacggtga tgtcgcggct gtcgcgcgcg cgcgaacaca tgcgggtgct
     2461 gatgaccgaa gggccggttg aagggggcgc cgggggactg gcgcgtaaag tcccgccgct
     2521 gaaagtagtg agaaatccgt gatgaataac gaccacgata ccagcttgcc tgaggggctc
     2581 gatctgcgag tgctgtcggc gttcgtcgac gacgagttga cgccggcgga gcgcgcggcg
     2641 atcgagacgc aactggcaca gcagccgcag gctgccgcga gagtggccgc atggcgcgcg
     2701 cagaaagcag ggctgcaggc gctgtgtggc gcgccactgc gcaacgagcg cggcgggggc
     2761 gccgggcaga gcgaggcgga tgagccggcc ttcatcgtgg tgcggcggcc ggcgccgtcg
     2821 tggcaacgca tgggtctcgc ggcgtgctgg cttgcgctcg gcgcgggcct cgcgcttgcg
     2881 ttggggccgc tcgcaccgcg cctgacgggc ggcgcgtgga acggtctggg cggccagccg
     2941 ccgagctttg ccgagcgcgc cgacatcgcc tacgcggtgt acacgcccga acagcgtcat
     3001 cccgtggaag tcgccgcgag cgaggaagag catctgatca actggctgtc caagcgcttg
     3061 aaccggccgt tgtcggtgcc gtcgttgcag gaatacgggt actcgctggt gggggggcgt
     3121 ttgttgcccg gcgaggcggg gccggccgcg cagttcatgt acgagaatcg gagcggcgcg
     3181 cgcctcacgc tgtatatcac cggcatttcg cgggacgaaa ccgcgttccg cctgttccgt
     3241 gacggcaacc gccgcacctt ctactgggtt agcgaccgca tgggttacgc gttgtccggg
     3301 ccgattgcgg aaggcaagct gcgctcgatc gctgtcgacg tgtgcagcgc gctcggcggc
     3361 aaaccggaga cgtggcagga gcgctgacgg cacggcgagt agggcaggcg cggataaccc
     3421 tggaagccct ggaagccctg gaagccgcag aagccgcaga agccgcagaa gccgcagaag
     3481 ccgcagaagc cgcagaagcc gcagaagccg cagaagccgc agaagccgca gaagccgcag
     3541 aagccgcaga agccgcagaa gccgcagaag cact
//
LOCUS       DATASH010000447        11020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5212245,
            whole genome shotgun sequence.
ACCESSION   DATASH010000447 DATASH010000000
VERSION     DATASH010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 11020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11020
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5212245"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="VHV99_12325"
     CDS             <1..420
                     /locus_tag="VHV99_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX3636256.1"
                     /translation="KLGNIPPQDIEAAFLGVFDGIVPESTRFLDGETIGALFDRVLPA
                     VAALRADTSWDTVLLVLHGGVNRAILSHALTAGGRTFFGHLAQATGCINALDVGTAPR
                     DWVLRTINYSPPSPLHRDVRNTTMEMLYAQFIQYKRS"
     gene            440..1546
                     /locus_tag="VHV99_12330"
     CDS             440..1546
                     /locus_tag="VHV99_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX3636257.1"
                     /translation="MVQATQTGEPERKPDYSAFEGTRPVNERQRFDSDALAAWLSQHV
                     DGFAGPLTLEQFAGGQSNPTFKLVTPSRAYVMRAKPGPAAKLLPSAHAVEREYRVMHA
                     LAGTGVPVANMLALCEDESVIGRAFYVMEFVEGRVLWDQSLPGMTPAERAAIYDEMNR
                     VIAALHSVDVAAIGLADYGKPGNYFARQIGRWSKQYIASETEPIDSMQRLIEWLPQHM
                     PAETSERASVVHGDYRLDNLIFHPTEPRVLAVLDWELSTLGDPLADFAYHCMAWHVDP
                     AQFRGIAGLDWAALGIPDEAQYVERYCKRTGFEIHGDWNFYLAYNMFRIAAILQGIMK
                     RVVDGTAASAQALDAGRRAKPMAELAWRYAQKVR"
     gene            1614..2873
                     /locus_tag="VHV99_12335"
     CDS             1614..2873
                     /locus_tag="VHV99_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX3636258.1"
                     /translation="MNFDYTPKVQALREKLLAFFDEHIYPNEQAFYAEIARNRQNGNA
                     WLPTELIEQLKQKARDAGLWNLFLPESVRGAGLTNLEYAPLCEIMGRVPWAPEVFNCN
                     APDTGNMETIERYGSDDNKREWLEPLLQGQIRSAFLMTEPDVASSDATNIQTSIVRDG
                     DYYVINGHKWWSSGAGDPRCKVYIVMGKTDPEAPRHAQQSMILVPAEATGITVHRPLT
                     VFGYDDAPHGHMEITLENVRVPATNMLLGEGRGFEIAQGRLGPGRIHHCMRLIGLAER
                     AIELMAKRSLQRVAFGKPVAAQGVTQERLAEARCMIEQARLLTLKTAYMMDTVGNKGA
                     RGEIAMIKVVAPNMACQVIDWAIQAHGGGGVSDDFPLAYAYASARTLRFADGPDEVHR
                     NAIAKLELARYMDAGAAARVETPITRV"
     gene            3130..3831
                     /locus_tag="VHV99_12340"
     CDS             3130..3831
                     /locus_tag="VHV99_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011545808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HEX3636259.1"
                     /translation="MIDVYSWATPNGHKVHIMLEETGLAYTAHAVNIGAGDQFTPEFL
                     AISPNNKIPAIVDPEGPKGADGKPFALFESGAILIYLAEKTGKFLPTDPAARYATLQW
                     LMFQMGGLGPMLGQTHHFRIYAPEPVEYAINRYTNETRRLYGVMDTQLGKTQYLAGND
                     YTIADIAAFPWTRSWQNQGVELDALPNVKRWHEEIAARPAVVRGVDVLASARKPLMDD
                     KAKEILFGATQYAKR"
     gene            complement(3980..4456)
                     /locus_tag="VHV99_12345"
     CDS             complement(3980..4456)
                     /locus_tag="VHV99_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX3636260.1"
                     /translation="MTGSAMPAATFGDAAALRALIGAEPLVSDWLRVDQASVDRFAEA
                     TGDHQWIHVDPERARRESPFGGPVAHGFMTLSLIPALLGKTVALKQRMGVNYGLNRVR
                     FTSPVLVGSQLRARFAVESVTDVDNAGVQVEWNVTLERQGSERPVCVAAFITRHYF"
     gene            complement(4463..4909)
                     /locus_tag="VHV99_12350"
                     /pseudo
     CDS             complement(4463..4909)
                     /locus_tag="VHV99_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002173.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
     gene            complement(4944..6071)
                     /locus_tag="VHV99_12355"
     CDS             complement(4944..6071)
                     /locus_tag="VHV99_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX3636261.1"
                     /translation="MDFTFNDEQQQFADALRRYLDKSYGFEARQAIVQSEAGVSDAHW
                     AAFTELGLTALPVPEAQGGFNGSPIDMLVVMQELGRALVIEPYWATAVGIEALRLAGT
                     QQGDDASLLERAAQGEFKLAVAFHEPHARYDLFEVETVASGQRDQQTLTGTKSVVLHG
                     AQADYWIVPARLNGEIALFVVARDAAGVAVTDYRTIDGQRAATLKFNGTPARQLTGKH
                     AGAAALEHIADYGTVLLCAEAVGALDALNHATVEYTRTRQQFGQPIARFQALQHRMVE
                     MLIHAEQARSVTYLAAMRYTSAVADERRRAVSAAKARVGQAARFVGQQAVQLHGGMGV
                     TNEVAAAHLFKRLAIIETTLGDVDHHLARFSALPGFVIAEA"
     gene            complement(6082..7278)
                     /locus_tag="VHV99_12360"
     CDS             complement(6082..7278)
                     /locus_tag="VHV99_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006763850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX3636262.1"
                     /translation="MNLDYSPADGAFRADIRAWLEANLPRELSDKVLNHKRLNREDFA
                     GWHKLLGTRGWSVVAWPKEYGGPGWDATQRHIWDEECARIGAPSVLPFGVSMVAPVLM
                     KYGNEAQKRHYLPRILDGTDWWCQGYSEPGSGSDLASLRTRAERVGDHYVVNGQKTWT
                     TLGQYADMMFCLVRTDSGAKKQEGISFLLIDMKTPGITVRPIITLDEDHEVNEVFFED
                     VKVPVENLVGEENRGWTYAKYLLGHERTGIARVGQSKRELVFLKRVALDQKKNGKPLL
                     QDPVFAAKVASLEIELMALEVTVQRVVANEAGGRGPGPEASMLKIKGTEVQQALTELM
                     FEAIGPLAAPFDVPFLEGEREHSLAGDDDAAPLAAYYFNFRKTSIYGGSNEIQKNIIA
                     QMILGL"
     gene            complement(7327..7752)
                     /locus_tag="VHV99_12365"
     CDS             complement(7327..7752)
                     /locus_tag="VHV99_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HEX3636263.1"
                     /translation="MKVLDLQCPHGHRFEGWFASADDFESQQSRKLIECPICGANEVS
                     RLPSAPRLNLSGATETKVPAGAAQMQARVMRALREVLEKTENVGDRFAEEARRIHYNE
                     APARNIRGVTTPEDAKALVEEGIEVMPLPVPAALKEPLQ"
     gene            complement(8005..8529)
                     /locus_tag="VHV99_12370"
     CDS             complement(8005..8529)
                     /locus_tag="VHV99_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEX3636264.1"
                     /translation="MEYVFTLKYRLAAEDRDLDEIVERLGEAGCDDATIGVGQPGRIA
                     LVFAREGASAFEALVSALKDVKQVVPSARLVEAGPDFVGLTDVAEVAGVSRQNMRKLM
                     LSHAIDFPPPVHEGSASVWHLSDILGWLTARGGYDIKADVFDVAKSAKQINLAKEALE
                     LEPRLNRRLESLVA"
     gene            complement(8580..8828)
                     /locus_tag="VHV99_12375"
     CDS             complement(8580..8828)
                     /locus_tag="VHV99_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636265.1"
                     /translation="MVRGIHSRKEVEAALAYAERNGWRVQGGGKGHAWGKMYCPYTDA
                     ECRCGEFCISSVWSTPKNPGNHARHLRRIVDNCTTRRQ"
     gene            complement(9165..9749)
                     /locus_tag="VHV99_12380"
     CDS             complement(9165..9749)
                     /locus_tag="VHV99_12380"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX3636266.1"
                     /translation="MAELPDHDAALTETCIESKTIHQGPFLTLKCDTVRLPDGKQAPR
                     EYVQHPGAVMVIPLFDDGRVLLESQYRYPMGKVMVEYPAGKLDPNEGALACAKRELRE
                     ETGYTAREYVYLTRIHPIISYSTEFIDIYLARGLTAGERKLDDGEFLELFTATVADVS
                     EWVRTGKITDVKTVIGTFWLEKVLSGTWPMAEAQ"
     gene            complement(9792..10088)
                     /locus_tag="VHV99_12385"
     CDS             complement(9792..10088)
                     /locus_tag="VHV99_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015601582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HEX3636267.1"
                     /translation="MSAAGWFIVLLALVGANLPLLNQRLFAAVPLKAVKKSAWIRIAE
                     LIVLYFVVGALGFLLEARAGNRFEQGWQFYAVTFALFVVFAFPGFTFQYLVKRR"
     gene            complement(10088..>11020)
                     /locus_tag="VHV99_12390"
     CDS             complement(10088..>11020)
                     /locus_tag="VHV99_12390"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEX3636268.1"
                     /translation="GATGSLDLNEVFKAIGTSHYDPSVLLFGVIFIVAGVAFKMGAVP
                     FHMWVPDVYQGAPTAMTLMVGGGPKVAAFAWGLRFLVMGLLPLAVEWQEMLVILAALS
                     LIVGNITGIVQRNVKRMLAYSAISNMGFVLLGLLAGVVDHKTTGAANAYGSAMYYSIV
                     YLITTMGTFGIIMLLARRDFEADTLEDFKGLNQRSPVFAFVMMVMMFSLAGIPPAVGF
                     YAKLAVLQATMNAGLTWLTVLAVITSLFGAFYYLRIVKLMYFDEPQDKSPILADTSTR
                     ALLALNGVAVLVLGIVPDPLLKACLQAIQHTLLL"
BASE COUNT         2230 a         3509 c         3240 g         2041 t
ORIGIN      
        1 aagcttggca atattccgcc gcaggatatc gaagcggcat ttctcggcgt gttcgacggc
       61 atcgtgccgg aaagcacgcg ctttctcgac ggcgaaacca tcggcgcgtt gttcgaccgc
      121 gtgttgcctg cggtagcggc gttgcgcgcg gacacgtcgt gggacaccgt gctgctggtg
      181 ctgcacggcg gggtgaatcg cgcgattctg tcgcacgcgc tcactgcggg cggccgcacg
      241 ttctttggcc atctggcgca ggccaccggt tgcatcaatg cgctcgacgt cggcaccgcg
      301 ccgcgcgact gggtgctgcg cacgatcaac tattcaccgc cgtcgccgct gcatcgcgac
      361 gttcgcaata ccacgatgga aatgctctac gcacaattca ttcaatacaa gcgtagctga
      421 accgaactgg aggagacata tggtgcaagc cacacagacc ggcgaaccag aacgcaaacc
      481 ggattactcc gcctttgagg gcacgcgtcc ggtcaacgag cggcaacgct tcgacagcga
      541 cgcgctcgcc gcgtggctaa gccagcatgt cgacggattc gccggaccac tcacgctgga
      601 acaattcgcg ggcggccagt ccaatcccac cttcaaactc gtcacgccgt cgcgtgcgta
      661 cgtgatgcgc gccaagcccg ggccggccgc gaagttgctg ccctcagcgc acgccgtcga
      721 acgggaatac cgggtgatgc atgcgcttgc cggcaccggc gtgccggtcg cgaacatgct
      781 cgccttgtgc gaggacgaaa gcgtgattgg ccgggcgttc tacgtcatgg agttcgtcga
      841 gggccgcgtg ctgtgggacc agtctttgcc cggcatgacg cctgcggagc gcgcggcaat
      901 ttacgatgaa atgaatcgcg tgattgccgc gttgcatagc gtcgacgtcg cagcgatcgg
      961 ccttgcggac tacggcaaac cgggcaacta cttcgcgcgc cagattggcc gctggagcaa
     1021 acagtacatt gcatccgaaa ccgaaccgat cgactcgatg cagcgcctga tcgaatggct
     1081 tccgcaacat atgccggccg aaacgagcga gcgtgcctcg gtcgtgcatg gcgactaccg
     1141 gctcgacaat ctgatcttcc atcccaccga gccacgggta ctcgccgtgc tcgactggga
     1201 actgtccacg ctcggcgacc cgctcgccga cttcgcctat cactgcatgg catggcacgt
     1261 cgatccggcg caattccgcg gcattgcggg tctcgattgg gcggcccttg gcattcccga
     1321 cgaagcgcaa tacgtcgagc gctattgcaa gcgcaccggc ttcgagatcc acggcgactg
     1381 gaatttctat ctggcgtaca acatgttccg catcgccgcg atcctgcaag ggatcatgaa
     1441 acgtgtggtt gacggcaccg cggccagcgc tcaggcgctc gatgccggcc gtcgtgcaaa
     1501 gccgatggcc gaactcgcct ggcgctatgc gcagaaggtc cgctaaccga atcgaaccgg
     1561 cgcaccgggc tgcgctcccg aacaaacgtc attcgtcatc cgcgaggtca tacatgaatt
     1621 tcgattacac cccgaaggtt caggcgctgc gcgagaaact gctcgccttc ttcgacgagc
     1681 acatctatcc gaacgagcag gcgttctacg ccgagatcgc gcgcaatcgc cagaacggca
     1741 acgcgtggct gccaactgaa ctgatcgagc aactgaaaca gaaggcacgc gacgccggct
     1801 tgtggaacct gttcctgcct gaatcggtac gcggcgccgg tctgacgaat cttgaatacg
     1861 cgccgctgtg cgaaatcatg ggccgtgtgc cttgggcgcc ggaagtgttc aactgcaacg
     1921 cgcccgacac cggcaacatg gaaacgatcg agcgttacgg cagcgacgac aacaagcgcg
     1981 aatggctcga accgctgctg caagggcaga tccgttcggc gtttctgatg accgagccgg
     2041 acgtggcgtc atcggacgcg accaatatcc agacgagcat cgtgcgcgac ggcgactact
     2101 acgtgatcaa cggccacaag tggtggtcgt ccggcgcagg cgatccgcgc tgcaaggtct
     2161 acatcgtgat gggcaagacc gaccctgagg cgccgcgcca tgcgcagcaa tcgatgattc
     2221 tggtccccgc cgaggcgacc ggtatcaccg tgcaccgccc tctcaccgta tttggctacg
     2281 acgacgcgcc gcacggccac atggaaatca cgctcgaaaa cgtgcgcgtg ccggccacca
     2341 acatgctgct cggcgaagga cgcggtttcg aaatcgccca aggccgcctc ggaccgggac
     2401 gcatccacca ttgcatgcgg ttgatcgggc tcgccgagcg cgcgattgaa ttgatggcga
     2461 agcgctcgct gcaacgggtg gcgttcggca agccggttgc ggcgcaaggc gtcacgcagg
     2521 agcgtctcgc cgaagcacgc tgcatgatcg agcaggcgcg tctgttgacg ttaaagaccg
     2581 catacatgat ggacaccgtc ggcaacaagg gcgcgcgcgg cgagattgca atgatcaagg
     2641 tggtcgcgcc gaatatggcg tgtcaggtca tcgactgggc gatccaggcg cacggcggcg
     2701 gcggcgtcag cgacgatttt ccgctggcgt atgcgtatgc atccgcacgg accttacgct
     2761 tcgccgatgg ccctgacgaa gtgcatcgca acgcgatcgc aaagctcgag ttggcgcgct
     2821 atatggatgc gggtgccgcg gctcgcgtgg agacgccgat tacgcgggtt tgaggtcggg
     2881 gtcgaggcgg tagccggcgg cgcgaaatcg agactgcgca gagacacgca gcgctggatt
     2941 gcgaccgacc gggcggagcg tgacaacgcc acgcataggg ttgcggcgcg cgcaggcgta
     3001 gtgacgtgga ccgcggcgcc gcgcggatcg cgcgagttca cggccagcag catcgtccga
     3061 tctgtgctct aattttcgcc aactgcggcg ccctttgtgg cgccgcttcc acgaaacgaa
     3121 ggagccagga tgatcgacgt ctatagctgg gcaaccccga acggccacaa ggttcacatc
     3181 atgctcgagg aaacgggcct cgcatacacc gcacatgccg tgaatatcgg cgcgggcgac
     3241 cagttcacac ccgaattcct cgccattagc ccgaacaaca agattccggc gatcgtcgat
     3301 cccgagggtc ccaagggcgc cgacggcaag ccgttcgcgc tattcgaatc gggcgcgatt
     3361 ctgatttatc tcgccgaaaa aaccggcaag ttcctgccga ccgatccggc cgcacgctat
     3421 gcaacacttc aatggctgat gttccagatg ggtggattag gtcccatgct cgggcaaacg
     3481 catcacttcc gcatctacgc gccggagccg gtcgaatacg cgatcaatcg ctacacgaac
     3541 gaaacgcgac gcctctatgg cgtaatggat acacagctcg gcaagacgca gtatctggct
     3601 ggcaacgact acacgatcgc ggatatcgcc gcgtttccgt ggacccgctc gtggcaaaac
     3661 cagggtgtcg aactcgacgc gcttccgaat gtgaagcgct ggcatgaaga aattgccgca
     3721 cgcccggcgg tagtgcgcgg tgtcgacgtg ctggcgtcgg cccgcaagcc gctgatggac
     3781 gataaggcca aggaaatatt attcggcgcg acgcagtacg cgaagcggta gtcgtaccgt
     3841 cgcgccgtgg catcggcgct cgttcgcgtt gtcaatgacc gcagcgtcat tggtatccat
     3901 acgtgcgtcg acataaaaag cgcgagaggg catcactgcc actcgcgttt ttctcaaccg
     3961 ggcggttcgg tcatgaacgc tagaaatagt gtcgcgtgat gaacgccgcg acacataccg
     4021 gtcgctcact accctgccgc tccagcgtga cattccactc gacctgtaca ccggcgttgt
     4081 cgacgtcagt gacggattca actgcaaacc gtgcacgcag ttgcgaaccg accagcaccg
     4141 gcgacgtgaa ccgcacgcga ttcaagccgt aattgacgcc catgcgctgc ttgagtgcca
     4201 ccgtcttgcc gagcagcgcg ggaatcagcg acaacgtcat gaacccatgc gcgaccggac
     4261 cgccgaacgg cgattcacgc cgcgcccgct cggggtcgac gtgaatccat tgatgatcgc
     4321 cggtggcctc agcgaaacga tcgacgctcg cctgatcgac cctcagccag tcgctgacga
     4381 gcggttcggc gccaatcagc gcgcgcaacg ccgccgcatc gccaaacgtg gcggcgggca
     4441 tcgcgctgcc cgtcacgacg cgtcccggat tgcgcagtcc gaacaaagcc ttggagcgta
     4501 cggtgatcac cgtttcgccg tgctggttga tgccttccca ctgcgtcgaa acgatgccgc
     4561 gatccggctt actctccgag acgcgtttgt cgagtacggc gttcatcatg gtgatcgtgt
     4621 cgccgacgcg caccggcttt aaccagcgaa tttcgtcgat gccgggcgag cccatcgacg
     4681 tggaacccgc gatcgcgttg cgcaccagca tgcccatcat cactgaacag gtgtgccagc
     4741 cgcttgcgac cagtccgcga aacggcgacg ccgcagctgc agcctcgtcc aggtgaaacg
     4801 gctgcggatc gaacttctcc gcgaactcga cgatctcttc gcgcgtgaaa gtatgcttgc
     4861 cgatttcggt agtcgtgccg actaccatgt cttcataact cagacccatc gtcacacctc
     4921 tttcgtccga ttcgagcggc tgatcatgct tcggcgataa caaagccggg cagcgcggaa
     4981 aaccgcgcga gatggtgatc aacatcgccc aacgtggttt cgatgatagc aagacgcttg
     5041 aacagatggg ccgccgcgac ttcgttcgtg acgcccatgc cgccgtgcaa ctggaccgcc
     5101 tgctgaccga cgaaacgcgc ggcctggcca acccgcgcct tcgcggcgga gaccgcgcgg
     5161 cgacgttcgt cggcgactgc gctcgtgtaa cgcatggccg cgagataagt gaccgaacgc
     5221 gcctgctcgg cgtgaatcag catctcgacc atgcgatgct gcaatgcctg aaaccgtgcg
     5281 atcggctggc cgaactgctg tcgcgtcctg gtgtattcga ccgttgcatg gttcaacgcg
     5341 tcgagcgcgc cgaccgcttc cgcacacagc aataccgtgc cgtagtccgc aatgtgttcc
     5401 agggccgcgg caccagcgtg tttgccggtg agttggcgag cgggcgtgcc gttgaacttg
     5461 agtgtcgcgg cgcgctggcc gtcgattgtg cggtagtcgg tcaccgcaac gccggccgca
     5521 tcgcgggcga cgacaaagag cgcgatctcg ccattcagcc tcgccggcac gatccagtaa
     5581 tccgcttgcg cgccgtgcaa cacaaccgac ttcgtgccgg tcaaggtctg ttgatcacgc
     5641 tgcccgcttg caaccgtttc gacctcgaac aggtcgtagc gcgcatgcgg ctcgtgaaat
     5701 gccactgcca gcttgaactc gccctgagcc gcgcgttcga gcagcgatgc gtcgtcgccc
     5761 tgctgcgtgc ccgctagccg tagcgcctcg atgcccaccg cggtcgccca atacggctca
     5821 atgaccaaag cacgcccgag ctcctgcatg acgaccagca tatcgatcgg gctgccgttg
     5881 aagccaccct gagcttccgg caccggcaaa gcggtcaggc cgagttcggt aaaggctgcc
     5941 cagtgcgcat ccgacacgcc ggcttccgat tgaacgatcg cctggcgcgc ttcgaacccg
     6001 tagctcttgt ccagatagcg gcgcagcgcg tcagcaaatt gctgttgttc gtcgttgaaa
     6061 gtgaagtcca tgcgccgctc ctcaaagtcc cagaatcatc tgcgcgatga tgttcttttg
     6121 aatttcgttc gagccgccgt agatcgacgt cttgcggaag ttgaagtagt acgcggccaa
     6181 cggcgcggcg tcgtcgtcac cggcgaggct atgttcgcgt tcgccttcga ggaatggcac
     6241 gtcgaaaggt gcggcaagcg gcccgattgc ctcgaacatc agttcagtca gtgcttgctg
     6301 cacttccgtg cccttgatct tgagcatgga cgcctcgggt cccggcccac gtccgcccgc
     6361 ttcattggcc accacgcgtt gcacggtgac ttcgagcgcc atcagttcga tctcgagact
     6421 cgcgactttg gcggcaaaca caggatcctg cagcaacggt ttgccgttct tcttctgatc
     6481 cagcgcaacg cgcttcagaa atacgagttc gcgcttcgat tgaccgacgc gagcgatacc
     6541 cgtgcgctcg tgcccgagca gatacttcgc gtaagtccag ccacgattct cctcgccgac
     6601 cagattttcg accggcacct tcacgtcttc aaagaagact tcgttgactt cgtggtcttc
     6661 gtcgagtgtg atgattgggc gcacggtaat accgggcgtt ttcatatcga tcagcaggaa
     6721 agagatgccc tcctgctttt tcgcgccgct gtcggtacgc acgaggcaga acatcatgtc
     6781 cgcgtactgg cccagcgtgg tccaggtttt ctgaccgttg accacgtagt gatcgccaac
     6841 gcgctcggca cgcgtgcgca gcgaagccag gtcggaaccg gagcccggtt cggagtagcc
     6901 ctgacaccac cagtccgtac cgtcgagaat gcgcggcaga tagtggcgtt tctgcgcctc
     6961 attgccgtac ttcatcagca cgggcgccac catcgaaacg ccgaacggca gcacggacgg
     7021 cgcgccgata cgcgcgcact cttcgtccca gatatgccgt tgggtcgcgt cccagcctgg
     7081 gccaccgtat tcctttggcc atgcgacgac cgaccagccg cgcgtgccga gcagcttgtg
     7141 ccagccagcg aagtcttcgc ggttcagacg cttgtggttg agtaccttgt cgctcagctc
     7201 gcgaggcaaa ttcgcttcga gccaggcacg gatgtcggcg cggaacgcgc cgtcagcggg
     7261 ggaatagtcc agattcatgc gtagtgtctc ctggccgcga ccgcccgccg ccagtgagcc
     7321 actgcgctat tgcagcggtt ccttcaaagc agccgggacc ggcagcggca tcacttcgat
     7381 gccttcttcg accaaagctt tcgcgtcttc cggtgtcgtg acaccgcgaa tattgcgtgc
     7441 aggcgcttcg ttgtagtgaa tgcgccgtgc ttcctcggcg aagcggtcac caacgttctc
     7501 agtcttttcc aatacctcgc gcaatgcgcg catcacacgc gcctgcatct gcgccgcgcc
     7561 cgcagggact ttcgtttcag tcgcccccga cagattcagc cggggcgccg acggcaaacg
     7621 gctcacttcg ttcgcgccgc agatcggaca ttcgatgagc ttgcgggact gctgcgactc
     7681 gaaatcatcc gccgaagcga accagccttc gaaccgatgg ccatgcggac actgtaaatc
     7741 gaggaccttc atgtggaatc agggcttgcg tgccagttta gcgcaaaatg gaaatttgtg
     7801 aacggtcgtt cgtaaatttt tacggacagc acgttgacgc caggcgtcgc cgacaaatca
     7861 aacgcggtgc gcgattttaa cgctttgtgc aaccggctgc cgaaggtggc tcaagcagcg
     7921 aacggcagat cgaaaacccg tagatgaaag agcgcgctga acgcatggcg ctcatgagtt
     7981 cgcgcgctta ccgccgcgca ccttttaagc cacaagggat tcgagtcggc ggttaagccg
     8041 cggctcgagt tcgagcgcct ctttggcgag gttgatctgc ttggccgatt tcgcgacatc
     8101 gaacacgtcc gccttgatgt catacccgcc acgcgcggtc agccaaccaa ggatgtccga
     8161 cagatgccat accgacgcgc tgccctcgtg aaccggcggt ggaaaatcaa tggcatggct
     8221 cagcatcagc ttacgcatgt tttgccgtga tacgcccgcg acctcagcga catccgtaag
     8281 gccgacgaaa tccggccccg cttcgacaag ccgtgcggac ggcacgacct gtttgacatc
     8341 tttgagcgcg ctgacaagcg cctcgaacgc tgacgcgccc tcgcgtgcaa aaaccagcgc
     8401 aatgcgccct ggctgcccta cgccgatcgt agcatcgtcg catcccgctt cgcccagccg
     8461 ctcgacgatc tcgtcaagat cgcgatcttc agcggcaagc cggtacttca aggtgaacac
     8521 atattccata agctactcct gccctcattc agcttcgggc ttttgccctt cgcacacgct
     8581 tactgccggc gcgtcgtgca gttgtcgacg atgcgcctaa ggtgccttgc gtgattgccg
     8641 gggttcttcg gcgtactcca cacactcgaa atgcagaact cgccgcagcg acactccgcg
     8701 tcggtgtaag ggcagtacat ctttccccac gcgtgacctt taccgccgcc ctgcacacgc
     8761 cagccgtttc tttccgcgta agccaacgcg gcctcgacct ctttcctgga atggattcca
     8821 cgaaccatct atgccctcca atttaatgcg tgcatgtcag gttgtcaagt gacaacttat
     8881 ctgtatgacc gcagtcgaaa accatctcac gtccttcgtt atccaaaata agtgcgataa
     8941 caaactccgt tgccaggcat cgatagtaga acggcagcgg acagggggag agactgtcag
     9001 atggcatagg aggaaagccc acttttcaga cacggcgtaa cgcatggagt cgatatcgtg
     9061 cccacaatgg caaagcccct ggtggccata gaccaacagg ggcttttgcg gaatttccag
     9121 ccggcgcgcg actcgcgccc gttctgcgtt ctgaacagtg cgcgctattg cgcctcggcc
     9181 attggccagg tacccgacaa caccttctcc agccagaacg tgccaatgac ggttttcaca
     9241 tcggtgatct tgccggtgcg cacccactca gacacgtcgg ccacagtcgc ggtgaacagt
     9301 tcaaggaatt ccccgtcgtc gagtttgcgc tcgccggctg tcaacccgcg cgccaggtaa
     9361 atatcgatga attcggtcga gtaggaaatg atgggatgaa tgcgcgtcag ataaacatac
     9421 tcgcgcgcgg tatagccggt ctcttcccgc aactcacgtt tcgcgcaagc cagcgcacct
     9481 tcgttcggat cgagcttgcc cgccggatat tcgaccatca ccttgcccat cggataacgg
     9541 tactgacttt ccagcaacac acggccgtcg tcgaatagcg ggatcaccat cacggcgccc
     9601 gggtgctgaa catactcgcg aggagcctgc ttaccgtccg gcaagcggac ggtatcgcac
     9661 ttgagcgtga ggaacggccc ttgatggatc gttttgctct cgatacaggt ctcggtaagc
     9721 gcggcgtcgt gatcgggaag ttcagccata tgcgacctca gaacagcttg gtgcgcgacg
     9781 gcgagacgcc gtcagcggcg tttgacaaga tactggaacg taaagccggg gaatgcgaac
     9841 acgacgaaca atgcgaatgt gaccgcgtag aactgccacc cctgttcgaa acggttaccc
     9901 gcgcgtgctt ccagcagaaa accgagcgcg ccgaccacaa agtacagcac gatcaattcg
     9961 gcaatccgga tccaggcgct cttcttcacc gctttcaacg gcacggcggc gaagaggcgc
    10021 tgattcagta acggcaggtt ggcgccgacc agcgccaaca acacgataaa ccaacccgca
    10081 gccgacatca gagcagcagc gtgtgctgga tagcctgcag gcaggccttc aacagcggat
    10141 ccggcacgat accaagcacc agcaccgcaa caccattgag tgccagcaac gcacgcgtgc
    10201 tcgtgtcggc gaggatcggc gacttgtctt gcggttcgtc gaagtacatc agcttgacga
    10261 tacgcaggta gtagaacgcg ccgaacagcg acgtgatcac ggccagcacg gtcaaccacg
    10321 tcaacccggc attcatggtt gcctgcaaca ccgcgagctt ggcgtagaaa ccgaccgcag
    10381 gcggaatgcc ggcgagcgag aacatcatca ccatcatgac gaaagcgaac accgggctac
    10441 gttgattcag gcccttgaag tcttcgagcg tgtccgcttc gaaatcgcgg cgtgccagca
    10501 gcatgatgat gccgaaggtg cccatcgtcg taatcaggta gacgatgctg tagtacatcg
    10561 ccgagccgta cgcgttggca gcacccgtgg tcttgtggtc cacgacgccc gccagcaggc
    10621 ccagcaacac gaagcccatg tttgaaattg ccgaatacgc aagcatccgc ttgacgttgc
    10681 gttgcacgat accggtaatg ttgccgacga tcagcgacag cgcagcgagg atgacaagca
    10741 tttcctgcca ttcgaccgcc agcggcagca aacccatcac gaggaagcgc aggccccacg
    10801 cgaatgccgc cactttcgga ccaccgccga ccatcagcgt cattgccgtc ggcgcaccct
    10861 gatacacatc gggcacccac atgtggaacg gcaccgcacc catcttgaac gccacgcccg
    10921 ccacgatgaa gatcacgccg aacagcagca cgctcggatc gtagtggctc gtgccgatcg
    10981 ccttgaacac ttcgttcagg tcgagcgagc cggtcgcgcc
//
LOCUS       DATASH010000448         9611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3432249,
            whole genome shotgun sequence.
ACCESSION   DATASH010000448 DATASH010000000
VERSION     DATASH010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 9611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9611
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3432249"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="VHV99_12395"
     CDS             complement(<1..785)
                     /locus_tag="VHV99_12395"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX3636269.1"
                     /translation="MWRTFLLVCMAGAIQAQSGSVHAAMQRDAAEHGAGAARTAAASI
                     APLARQRKDTQPARIALVIGNGAYGDHAAYKDPLRENAPRDAEAMRDTLHARGFDVIM
                     RTDATPQQMREAINEVRQRLRDGGIGLFYFAGHGLQVGRQTLLVPAGLDARAPARLVS
                     EGVDLDSVIQAMRAPRAGQLNVVILDACLNNPFSADVTFDTAALPANTVVAYATTPGG
                     FAADGLRHGVYTNTLLRALNDASSSQDLAGLFQRVATTVSALT"
     gene            1197..1700
                     /locus_tag="VHV99_12400"
     CDS             1197..1700
                     /locus_tag="VHV99_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4399 domain-containing protein"
                     /protein_id="HEX3636270.1"
                     /translation="MRRVLTPGVLESRYGANAWLAALRPALRVTLLILLFGASLCEAA
                     LAGQTPSPPGAEEYIIWPSDGTVIHGGKLWVRMGLRNMGVCPKGVAVPKTGHHHLLID
                     TDLPPLDQEIPSDRNHLHFGAGETDARIELPPGKHTLQLILGDQNHVPHVPPVYSKKI
                     TITVLKD"
     gene            1747..2181
                     /locus_tag="VHV99_12405"
     CDS             1747..2181
                     /locus_tag="VHV99_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4399 domain-containing protein"
                     /protein_id="HEX3636271.1"
                     /translation="MYKIIALAACLALASLAAPNLATSGTTPAPAGAHAYIGYPNDGQ
                     VVPANKPFRVWFGLRYMGVAPKGVKYPNTGHHHLLIDTDLPPMDQEIPSDRNHLHFGA
                     GETETMIQLPPGKHTLQLLMGDDMHIPHNPPVYSKKITVIAR"
     gene            2465..2743
                     /locus_tag="VHV99_12410"
     CDS             2465..2743
                     /locus_tag="VHV99_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636272.1"
                     /translation="MNTLMIKDLSITEQLDSKAMSAVRGGFGYPSSSYFNFTPVYAPN
                     NSKTVNATQLISNELNIQNANGNNAAFVAGITTTIDPHVSSSNNINVH"
     gene            2991..4475
                     /locus_tag="VHV99_12415"
     CDS             2991..4475
                     /locus_tag="VHV99_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEX3636273.1"
                     /translation="MGGQRFFNASSPGCPERRAARVNSAAAALRGLCAATVCALSLCA
                     CIDVSMPDYKRPDTPAKASWSDQKGSPVSAAATIDPDWWKGFHDPYLNTLIAKAIANN
                     FDIKVLAARIDVAGTQIGEAKAGALPTMDLGVGADFEKTTGQTFSKQYNVATQVSWDI
                     DIWGKVEKGVQAQKAEFHASEADWRAGYLELVANVSSTYFQILQFDDQIEQQQQTLVT
                     NRQILTIYDGQRRNGLVPQTQVLRQQAEINRLTNQLLELRRSRAIANNALCTLLGVPA
                     GEFQLPKGHLQQRVQLPAVPDGLPAQLLARRPDVVAAEFRVLEAYDLVGQAKLAQLPT
                     INLTGHAGTASFALTDLLKSFTYGFMPSINIPLLDPGVRAHVKVTQAQSTVAEQQYRV
                     AVMGAFEEVENALVNLSSHKEQRLELQQEVSRLQIVADQIQSQLRLGVVSQLEVFETE
                     RTLLEAQQELLANHQLILSDTVLLYKALGGGWPSVDVQTEVKEH"
     gene            4705..7302
                     /locus_tag="VHV99_12420"
     CDS             4705..7302
                     /locus_tag="VHV99_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX3636274.1"
                     /translation="MTAGEASIAEMRATQDATFDVVLSPVASAQRPALDAIRIVDSLF
                     AIGRSEVPFTDYPAERITRLSRRHARIFTEHGAVYVADLGSKNGTTLNGVPVRQAPAR
                     VRGGDELCFGGELCYRVGIEPRARIVAAASAPPAPGLLLEPQRGDLDLQPIDVQAFPF
                     LISKADEVFSRYRDRYPHQVNYISRRHAHVFLKGGELYLEDLGSTNGTFVGGKRLDET
                     AVALVEGDLLAFGGDHFVYRVTLQKPPEVEPTVTQLFLNPAVDDADTDKTTFVGAANS
                     FLDIFCVDPGLQREDEVNEAAQAVSAQAKRDTLTGAANGTKGKNGTDGAHGTGSTHAS
                     HGTKVAKGRPQRWRLLASELSKAFAGSDHTMARRAAWWGGAAVVILVAMALTLYLRGS
                     SERELRNLLASGDYTDAVTAANGYLATHPTDTKVSALASEALLKAKIPGWLNALHKAQ
                     FDQADSLLKEMRSLSANNADGSALVGELQWVGDLERFVAGRGGVDAPIRMYTDEATIN
                     GLLERWDDDARSHQRALDRIASYVPVFAEPYAQALSHLRKLESDDSVYLAAIDRLNGT
                     IRTELARDKPDALPAVFDDYAQRYPRLAGLDRVRADLRQYTDVLNAALGRQLTALLAL
                     LKTVRFSTPPFQAQFQQLAASRLPSPDVIRRHDAATSAWQHGDAQQALADLQAMPAGP
                     WSDVIAAELAHKKALLDQFADVQKSRGGKDYDQRLLSFYASLDPTADAWFVQSIQKDV
                     AALHDKALARAQDLLLRAQSLWKQYRASGAIGGTQRLEAGISPAFRSEARLLSDAQSA
                     AQQGMRIYTQLKADHPADFDRLLADIEAEADLQRRSLTELRMVLDPGLLKAKLALIGG
                     EQREARQSP"
     gene            7280..8653
                     /locus_tag="VHV99_12425"
     CDS             7280..8653
                     /locus_tag="VHV99_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX3636275.1"
                     /translation="MKRDSHPRPLSEALEDHSVEGIAILTAEPVRLARALIWAMVELV
                     VAGLLWSFVGRADVIVSAQGTLSPESEVRRIYAPIDGELADLYIAEGQPVQKGDVLAR
                     LNARGAIEAASNALQAQLKLEDAERDWKQFPEKKVLMERKAAALKAQMEVEAHLHENR
                     VSEGTSKLAEGQKAELDEARSVLDNARRTREAARQELDRYSRLFAQPGGGGIAELQVE
                     QKRTAAMEADNAYRVAQSKLAELDFRLSHEYAQANAQLETSGQQTTDLQLQYDSAVRD
                     ITDAEDKVRLQLQTARLVAEAAARIRFENIDKDNFLLILAPVSGVITDVTSTQRGDKI
                     QANAPLGGIAPKDAKPVLKIEIAEHDRAFLHEGQSVKLKFNAFPYQRYGLISGTLAYI
                     SPATKPSLQDKQPVYEGRVTLDKNYYQIADTRYPLRYGMTASAEIVVRERRLIDLGLD
                     PFRQVAG"
     gene            8691..9443
                     /locus_tag="VHV99_12430"
     CDS             8691..9443
                     /locus_tag="VHV99_12430"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX3636276.1"
                     /translation="MTAIVRIDDEVVDVSEFIRLLKLTGQFESLIEQIVRDKLTVHAA
                     KKHGVAVTADEIQQRADQFRRVRGLHRATDMNQYLDALNVSLDEFEAFITDGLYQEKM
                     LDVVGNEAAIKDYFALNSPKFDAIEVSHIVLDSEGKAKEMISYLHDDPDSFADMAREH
                     SIADTREAGGVIGKVLRGSLKPDIEAKIFNAAVGDLLGPFPSADRSCFEIFAVTAKYP
                     ATLDADVAAEVRRLLRESWLIARAQEHVIEAR"
BASE COUNT         1966 a         2979 c         2954 g         1712 t
ORIGIN      
        1 ctggtgagcg cgctaacggt cgtcgcgacg cgttgaaaca accccgcaag atcctgtgat
       61 gaagaagcgt cgttgagcgc gcgcagcaac gtatttgtat agacgccgtg acgcaggccg
      121 tccgccgcaa atccgccggg cgttgtcgcg tacgcgacca cggtattggc gggcaaagcg
      181 gccgtatcga acgtgacgtc cgctgagaaa ggattgttca ggcaagcatc gagaatcacg
      241 acattcagct gtccggcacg cggcgcgcgc attgcctgta ttacgctatc cagatcgacg
      301 ccctcgctaa ccagccgggc aggcgcgcgt gcgtcgaggc cggcgggcac gagcagcgtc
      361 tgccggccca cttgcaggcc gtgtcccgcg aaatagaaga gtccgatgcc gccgtcccgc
      421 agacgttgac ggacttcgtt gatggcttcc cgcatttgct gcggcgtcgc atcggtgcgc
      481 atgatgacgt cgaagccgcg agcatgcagc gtgtcgcgca tggcttcagc gtcgcgaggc
      541 gcattctcgc gaagcgggtc tttataagcg gcgtgatcgc cgtacgcgcc gttgccgatc
      601 accagtgcaa tgcgcgcagg ctgcgtatcc ttcctttgcc gtgcgagcgg cgcgatggac
      661 gccgccgccg tgcgtgcagc ccccgcgcca tgctccgcgg cgtcacgctg catcgcggca
      721 tggacactgc ccgactgcgc ctgaattgcc ccggccatgc acaccagcag gaatgttcgc
      781 cacatattcg tatcctgttt gttccggaca gaacataggg tttcgacgac cgcgacgccc
      841 gcgtcagccg ttgaagcgga tcgcacacgg catcgatgcc gcgcgcgact ctccatcgac
      901 aacctatgaa taacgatagc acgcgctttg cgcgtcacaa ctaactgtcc gcgcatcgtg
      961 aatgcgttat acggttaacg agtgtggcgt atctttttgg caagttacga cctaagctga
     1021 ttcacaaagt agggcactac ggccgtttta atcctgtggc cgctcatcta cgtcaatccg
     1081 ctacgtgaat cgacgcgtat ccacgtgaag cacgcgagcg cccgcgacat ccagtcggat
     1141 cgtccgcctg cgctttatgc agaggcggcc acgacccatc ttcgggagag tgcgcaatgc
     1201 gaagagtcct cacacctggc gtactcgaaa gtcgttatgg agcgaatgca tggttggcgg
     1261 cgcttcgccc ggctttgcgc gtcacgctcc tcatcctgct cttcggcgcg tcactgtgcg
     1321 aagccgcgct tgccgggcaa accccttcgc cgcctggcgc cgaggaatac atcatctggc
     1381 cgtcggatgg cacggtgatt cacggcggca agctgtgggt ccgcatgggg ttgcgcaaca
     1441 tgggcgtgtg cccgaagggt gtcgcggttc cgaagaccgg tcatcaccat ctgttgatcg
     1501 acaccgactt gccgccgctc gaccaggaaa ttccgtcgga ccgcaatcat ctgcatttcg
     1561 gtgcgggtga aaccgacgcg cggatcgagt tgccaccggg caagcacacg ttgcaactca
     1621 ttctcggcga ccagaatcac gtgccgcacg tgccgccggt gtattcgaag aagatcacca
     1681 tcaccgtact aaaagactag ttgtcccgtt cattcaaagc gggtccgtca ggacggagag
     1741 atcaacatgt ataagatcat cgcgctcgcg gcgtgtctgg cattggcgtc actggccgcg
     1801 ccgaatctcg ccacgtccgg cacgacgccg gcgccggctg gcgcgcatgc ctatattggc
     1861 tatccgaacg acgggcaggt ggtgccggcc aataagccgt ttcgggtgtg gttcggtttg
     1921 cgctatatgg gcgtggcgcc gaaaggcgtc aagtatccga acacggggca ccatcatctg
     1981 ctgatcgata ctgacctccc gccgatggac caggaaattc catcggaccg caaccatctg
     2041 catttcggcg cgggtgaaac ggaaacgatg attcaactgc cgcccggcaa gcacacgctg
     2101 caactgctga tgggcgatga catgcacata ccgcacaatc cgccggtgta ctcgaagaag
     2161 atcacggtca ttgcacgttg atgcgggagc ggcgcgatgc ggttttcgct tcagtgtcac
     2221 gtcgcagcca acaccgtagc gcacattgtt ggctgcgctc caccaactcc aatctgtcgc
     2281 tccgtagccg tcgcactgca cgtgacgatc tgcgatcccc gcagcccttt attcacggcg
     2341 gttcggaaca tttcttagga tcgaaaagca aaactggcac agcccatgcg ttagtgacac
     2401 tcgagcagac aagatgctcg aacccaaccg caagactgtg atgttccctt ccaggagatt
     2461 taccatgaac acgctgatga tcaaagacct gtccatcacc gaacaactcg acagcaaggc
     2521 catgagcgcc gtgcgcggcg gcttcggcta tccctcgtcg tcgtacttca acttcacgcc
     2581 ggtgtacgcg ccgaacaaca gcaaaaccgt caatgccacc caactgatca gcaacgaatt
     2641 gaacatccag aacgccaacg gcaacaacgc agcgttcgtc gccggcatca ccaccacgat
     2701 cgacccgcac gtgagctcga gcaacaacat caacgtgcac taaacggcgt aagccgccgc
     2761 ggaggcgctc atgcctccgc gggacgcaca agcttgacgc atcaaaaaga cttcgcatcc
     2821 aatcgttctt tccacacgac cccagcgtca ccgcccgatc cccgcgccga ccttcccgta
     2881 ccgcttcgat ggtgaagccg ggcaggacat gctttactta aatagcgcgt tctcctcgtg
     2941 caacatggcc ggcgtcatcg gagaaaatcg tgtcagcgtt gtttgattca atgggcggtc
     3001 agcgcttttt caatgcttcg tcgcccggct gtccggagcg tcgagcggct cgtgtgaaca
     3061 gcgcggccgc ggcgctgcgt ggactctgcg ccgcaacggt ctgcgcgctc tcgctctgcg
     3121 cgtgtatcga tgtcagcatg cccgactaca agcggcccga cacgcccgcc aaggcgtcgt
     3181 ggtcggatca gaagggctcg ccggtatcgg cggccgcaac catcgatcca gattggtgga
     3241 aaggcttcca cgatccttac ctgaacacgc tgatcgcaaa agcgatcgcg aacaacttcg
     3301 atatcaaggt gctggccgca cgtatcgacg tggcaggcac gcagatcggc gaggccaaag
     3361 caggcgcgtt gccgaccatg gatctcggcg tcggtgccga tttcgaaaag accacgggtc
     3421 agacgttttc caaacagtac aacgtcgcga cgcaggtgag ctgggacatc gatatctggg
     3481 gcaaagtgga gaagggcgtg caggcgcaaa aggccgaatt ccacgccagc gaggcggact
     3541 ggcgcgccgg ctatcttgag ctggtggcga acgtctcgag cacctatttc cagattctcc
     3601 aattcgacga ccagatcgag cagcagcaac aaacgctcgt gacgaaccgc cagattctca
     3661 cgatctacga cggccaacgc cgcaacgggc tcgtgccgca aacgcaggtg ttgcgccagc
     3721 aggccgaaat caaccgcctg acgaatcagt tgctcgaact gcgccgttcg cgcgctatcg
     3781 cgaacaacgc gttgtgcacg ctgctcggcg tgccggcggg cgagttccag ttgcccaaag
     3841 ggcatttgca gcaacgcgtg caactgcctg ccgtgcccga cggcttgccc gcgcaacttc
     3901 tcgcgcggcg tcccgacgtg gtggccgcgg agttcagagt gctggaagcc tacgacctgg
     3961 tcggtcaggc caagctcgcg caattgccca ccatcaacct caccggtcac gccggcacag
     4021 cgagtttcgc gctgaccgat ctgttgaagt cgttcaccta cggattcatg ccgagcatca
     4081 acattccact actcgatccc ggcgtgcggg cgcatgtgaa ggtcacgcaa gcgcaatcga
     4141 cggtcgccga acagcaatac cgcgtggctg tcatgggtgc attcgaggaa gtggaaaacg
     4201 cactggtcaa tctgagttcg cacaaggagc agcgattgga attgcagcag gaagtgtcgc
     4261 gtctgcaaat cgtcgccgat cagattcagt cgcaattgcg cctcggcgtg gtgtcgcagc
     4321 tggaggtttt cgaaaccgaa cggaccttgc tggaggcaca acaggaattg ctcgccaacc
     4381 atcaactgat tctgtccgac acggtgttgc tttacaaggc gctgggcggc ggctggccga
     4441 gcgtggacgt gcaaacggaa gtaaaggaac actgagctaa tccggggcat gacacgcacg
     4501 cggcgcattt cggctgcatc tgacgcagga atggccctta ccaccgacga tccgaaaatt
     4561 gacacttgcc atgtgtgact taatattcgt aacaggccgg attctgcgcg gctcactgaa
     4621 aaaatccgcc attcgccgta gttcttcttt ccgttcctgt tgcgttctcg ccgcagcggg
     4681 taaacgatta gtgatccaga taaaatgact gctggcgaag cttcgattgc tgagatgcgc
     4741 gcgacacagg acgccacgtt cgacgtggtg ctgtcgccgg ttgcgtccgc gcagcgtccc
     4801 gcactcgacg cgatccggat cgtcgacagc ctgttcgcga tcggccgcag cgaggtgccg
     4861 tttaccgact accccgccga gcggattacc cgcctgtcac gccgccatgc gcggattttc
     4921 acggagcacg gcgccgtcta tgtggccgat ctcggcagca agaacggcac gacgctcaac
     4981 ggtgtcccgg tgcggcaagc gccggcacgg gtgcgtggcg gcgacgaatt gtgcttcggc
     5041 ggcgagctct gctatcgcgt gggtatcgaa ccgcgcgcgc ggatcgtcgc cgccgcgagt
     5101 gcgccgcccg cacccggcct gctgctggag ccgcagcgcg gcgacctcga tctccaaccc
     5161 atcgacgtgc aggcgttccc ctttctcatc agcaaggccg acgaagtctt ctcgcgctac
     5221 agggaccgct atccgcatca ggtcaactac atctcgcgcc gtcatgcgca tgtctttctg
     5281 aagggcggcg agctttacct tgaagatctc ggcagcacca atgggacatt cgtcggcgga
     5341 aagcggctcg acgaaaccgc cgtggcgctt gtagagggag acctccttgc atttggcggc
     5401 gaccacttcg tctacagggt gacgctgcaa aaaccccctg aagtcgagcc gaccgtgacc
     5461 cagttattcc tcaaccccgc cgtcgacgac gccgataccg acaagaccac gttcgtcggc
     5521 gccgcgaatt cgttcctcga tatcttctgc gtcgacccgg ggctacaacg cgaagacgaa
     5581 gtcaacgagg cggcgcaggc cgtttctgcg caggcgaagc gcgacaccct gacgggcgct
     5641 gcaaacggca cgaaaggcaa gaacggaact gacggcgcgc atggcacagg cagtactcat
     5701 gcgagccacg gcacgaaggt agcgaagggc cgcccgcagc gttggcgtct gctcgccagc
     5761 gaactcagca aggcctttgc gggtagcgat cacacgatgg cgcgccgcgc tgcatggtgg
     5821 ggtggcgcgg ccgtcgtcat tctggtggcc atggcgctga cgctctacct gcgtggctca
     5881 tcggagcgcg agttgagaaa cctgctggca agcggcgact acaccgacgc ggtgacggcg
     5941 gcaaatggct atctggcgac tcatcccacc gacacgaaag tcagcgcatt agcgagcgaa
     6001 gcgctgctga aggcaaaaat ccccggctgg ctgaacgcgc tgcacaaggc gcagttcgac
     6061 caggcggact cgctgctcaa ggagatgcgg tcattgagtg cgaataacgc ggacggttcc
     6121 gcgctggtcg gcgaactgca atgggtgggc gacctggagc gcttcgtggc gggacgcggc
     6181 ggcgtagacg cgccgatccg catgtacacc gacgaggcca ccatcaacgg cctgctggaa
     6241 cgctgggacg acgacgcgcg aagccatcag cgcgcactgg accgcatcgc ctcgtacgtg
     6301 cctgtttttg cggagccgta tgcgcaggcg ctgagccatc tgcgcaaact ggaaagtgac
     6361 gactcggtgt acctcgctgc catcgatcgc ctgaacggca cgattcgcac cgaactggcg
     6421 cgtgacaagc ccgatgcgct gcccgccgtc ttcgacgatt acgcgcagcg ctatccgcgt
     6481 ctcgccggcc tcgaccgtgt acgggcagac ctgcggcagt acaccgatgt actgaatgca
     6541 gccctgggcc ggcaactgac ggcattgctc gccttgctga agacggtgcg cttcagcacc
     6601 ccgccattcc aggcgcagtt tcagcaactg gccgcgagcc gtctgccgtc gccagacgtc
     6661 attcggcgcc atgacgcggc gacctcggcg tggcaacacg gcgacgcgca acaggcgctg
     6721 gccgatttgc aggccatgcc ggccgggcca tggtccgatg tgatcgcagc ggaactggcg
     6781 cataagaaag cgctgctaga ccaattcgcc gatgtgcaga aatcgcgcgg cggcaaagac
     6841 tacgaccaac gcttgctgtc gttctacgcc agtctcgatc cgacggcaga cgcgtggttc
     6901 gtgcaatcga tccagaaaga cgtcgccgcg ttgcacgaca aagcgctggc gcgcgcgcaa
     6961 gacctgctgc tgcgcgcgca gagcctctgg aaacagtatc gggcgagcgg tgcgatcggc
     7021 ggcacgcaac ggctggaagc ggggatttcg cccgcctttc gcagcgaggc gcgtctgtta
     7081 tccgatgctc aatctgccgc gcaacaaggc atgcgcatct atacgcagct gaaagcggat
     7141 catcccgccg acttcgaccg cctgctcgcg gatatcgaag ccgaagcgga tttgcagcgc
     7201 cgctcgctga ccgagttgcg catggtgctg gaccccggct tgttgaaggc gaagctggca
     7261 ttgatcggag gcgagcagcg tgaagcgcga cagtcaccct agaccgttgt cggaggcgct
     7321 ggaggaccat agcgtcgagg gcatcgcgat tctgaccgcg gagccggtac ggctcgcgcg
     7381 cgcgctgatc tgggcgatgg tcgagctggt ggtggccggt ttgctgtggt cattcgtggg
     7441 ccgtgccgat gtgatcgtca gcgcgcaggg cacgctctcg ccggaatcgg aggtgcgccg
     7501 catctatgcg ccgatcgacg gcgaactcgc cgatctgtac atcgccgagg ggcagccggt
     7561 gcaaaagggg gatgtgctgg cgcggctgaa tgcgcgcggt gcgatcgagg cagcgagcaa
     7621 cgcgctgcag gcgcaactca aactcgaaga tgccgaacgc gactggaaac agttccccga
     7681 gaagaaggtg ctgatggagc gcaaggcagc tgcgctgaag gcgcagatgg aagtggaagc
     7741 ccatctgcac gaaaaccgcg tctcggaggg caccagcaag cttgccgaag gccagaaggc
     7801 cgaactcgac gaagcgcgca gcgtgctcga caatgcgcgc cgcacgcgtg aagcagcgcg
     7861 gcaggaactg gaccgctact cgcggctttt cgcgcagcca ggcggcggtg gtatcgccga
     7921 gttgcaggtg gagcagaaac gcaccgccgc gatggaagcg gataacgctt accgtgttgc
     7981 acaatcgaaa ctcgcggaac tggatttccg cttgagccac gaatacgcgc aggccaatgc
     8041 gcaactcgaa accagcggcc agcagaccac cgacctgcaa ctccagtacg actcggccgt
     8101 ccgcgacatc accgacgcgg aagacaaggt gcgtctgcaa ctgcagaccg cacgcctcgt
     8161 ggccgaagct gccgcgcgaa tccgcttcga gaacatcgac aaagacaatt tcctgctgat
     8221 cctcgcgccg gtctccggcg tcattaccga cgtgacctcc acgcagcggg gcgacaagat
     8281 tcaggcgaac gcgccgctcg gcggcatcgc gccgaaagac gcgaagcccg tcctgaagat
     8341 cgagatcgcc gaacacgacc gtgcttttct gcacgaaggc cagtccgtga agctgaaatt
     8401 caacgcgttc ccgtatcagc gctatgggct gatcagcggc acgcttgcct atatttcgcc
     8461 ggccaccaaa ccgtccttgc aggacaaaca accggtgtac gagggccgcg tgacgctcga
     8521 caagaactac taccagattg ccgacacccg ctatccgctg cgctatggca tgacggcgag
     8581 cgcggaaatc gtggtgcgcg aacggcgcct gatcgatctg ggtctcgatc cgttcaggca
     8641 ggtggcgggt tgagcgcgcc gactacacgt caattaaagg agcggatgac atgaccgcga
     8701 tagtacgaat cgatgacgaa gtggtggacg tttccgagtt cattcgtctt ctgaaattga
     8761 ctggccagtt cgagagcctg atcgagcaga tcgtgcgcga caagctcacg gtgcatgcgg
     8821 cgaaaaagca cggcgttgcc gtcacggcgg acgaaatcca gcagcgcgcc gatcagttcc
     8881 gccgcgtgcg cggcttgcat cgcgcgacgg atatgaatca atacctcgat gcgctcaacg
     8941 tgagtctcga cgagttcgag gcattcatta ccgacgggct gtatcaggag aagatgctcg
     9001 acgtggtcgg caacgaggcc gcgatcaagg attacttcgc gctgaattcg ccgaagttcg
     9061 acgcgatcga ggtgagccat atcgtgctcg acagcgaagg caaggcgaag gaaatgattt
     9121 cgtacctgca tgacgatccc gacagcttcg ccgacatggc gcgcgagcat tcaatcgcgg
     9181 atacgcgcga agccggcggc gtgatcggca aggtgttgcg gggatcgttg aagccggaca
     9241 tcgaggcgaa aattttcaat gcggcggtgg gcgatctgct cgggccgttt ccgtcggcgg
     9301 atcgctcgtg cttcgagatt ttcgccgtga ccgcgaaata tccggcgacg ctcgatgcag
     9361 atgtggccgc cgaagtcagg cgtctgttgc gcgagagctg gctgattgcg cgcgctcagg
     9421 agcatgtgat cgaagcgcgc tagccgctgc gcgcggttcg cttcatagcg aaacgcgtca
     9481 cgagattcaa cgcaatcgaa gtcggccgaa cggagatccg caagcacatg gacgtacccc
     9541 agaccgcgtc ttctgccgcc gaatttctgg cctcggtaga aatcttctcg ccgttttcgc
     9601 gcgatgagat c
//
LOCUS       DATASH010000449         4942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3829417,
            whole genome shotgun sequence.
ACCESSION   DATASH010000449 DATASH010000000
VERSION     DATASH010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4942
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3829417"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VHV99_12435"
     CDS             <1..600
                     /locus_tag="VHV99_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013700139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HEX3636277.1"
                     /translation="ARSMLERALDAGVPCGWVTGDEVYGGDRHLRLWLESRGQSFVLA
                     VAKNEPLWWQGPDYVRADRIAEALPARAWRRLSAGAGAKGERLYDWALTPLWRLQITT
                     EERRFGHYLLVRRSLDEQREHAYYVVYAPRSKATRQTLVNVAGRRWEIETGFEATKGE
                     CGLDQYEVRQWQGWYRHITLALLAHAALVTLRVRAKKNT"
     gene            complement(955..1030)
                     /locus_tag="VHV99_12440"
     tRNA            complement(955..1030)
                     /locus_tag="VHV99_12440"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(995..997),aa:Thr,seq:cgt)
     gene            complement(1086..2102)
                     /locus_tag="VHV99_12445"
     CDS             complement(1086..2102)
                     /locus_tag="VHV99_12445"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEX3636278.1"
                     /translation="MALQRRTTLTKYLIEQQRETNNLPADLRLLIEVVARACKAISYH
                     VSKGALGDALGTAGSENVQGEVQKKLDILSNEILLEANEWGGNLAGMASEEMEQFFPI
                     PANYPKGEYLLVFDPLDGSSNIDVNVSIGTIFSVLRCPDGQQPTEQSFLQPGTQQVAA
                     GYAVYGPQTVLVLTTGNGVNCFTLDRELGSWVLTQSEMRIPVETREYAINASNERHWY
                     PPVEKYIGELKAGKDGSRQSDFNMRWIASMVADVHRILNRGGIFMYPADKRTPDKPGK
                     LRLMYEANPMAFIVEQAGGAATNGEKRILDIQPKSLHERVAVFLGSKNEVDRVTRYHL
                     ETKN"
     gene            complement(2223..2678)
                     /locus_tag="VHV99_12450"
     CDS             complement(2223..2678)
                     /locus_tag="VHV99_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HEX3636279.1"
                     /translation="MVIKRSDLDNIDFSDIGTGESIPEIHPGGILRSEFLEPLGMSVN
                     ALALALRVPAPRINDIVRGKRAISADTALRLERYFGASAQFWLNLQIGYDLRVATAAA
                     GAQIEREIEPMPKADRPKMPKVSPEHARAAALATSLTGNVTAIKARRKA"
     gene            complement(2695..2976)
                     /locus_tag="VHV99_12455"
     CDS             complement(2695..2976)
                     /locus_tag="VHV99_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX3636280.1"
                     /translation="MITTFGDEATAAIFEGKFVHSLPLYMQALARRKLLLIDAAESIR
                     SLHAPPGNRLEALQGQRSGQWSIRINAQWRICFHFVDGEALNVEIVDYH"
     gene            complement(3173..>4942)
                     /gene="pepN"
                     /locus_tag="VHV99_12460"
     CDS             complement(3173..>4942)
                     /gene="pepN"
                     /locus_tag="VHV99_12460"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011656238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEX3636281.1"
                     /translation="NRVTCRDWFQLSLKEGLTVFRDQEFSADMAGGATDEAARATKRI
                     EDVRVLRQMQFAEDAGPMAHPVRPESYVEINNFYTMTVYEKGSEVVRMYQTLFGRDGF
                     RKGMDLYFNRHDGQAVTCDDFRHALADANGRDLAQFERWYSQAGTPRVSVRTRYDAAQ
                     KRYSMTLTQGYGEGTATARETQKGPLLIPFAIGLIGKNGTDLPLQLEGEAQAGKATTR
                     VLEFTQSEQTFTFVNVAQEPLPSLLRNFSAPVIVEYDYSADQLAFLLAHDSDPFNRWE
                     AGQRLATRELLTLAGRAATGVPLQLEDSVVAAFARVLTDESLSPSFRELALMLPSEAY
                     LAEQMAESNPAAVHAARQFVRKRLANALRKDWLAVYEKHRTPGTYEATPEASGHRALK
                     NLALSYLAELDDPAEAVRLASAQYDAANNMTDRSAALSALLNAAAANGGSVQARQALD
                     DFYRRFEKEPLVIDKWFALQATQRGSAQRPVIEIVRKLMAHPAFNLKNPNRARSLIFS
                     FCAANPSQFHAEDGSGYAFWAEQVIALDAINPQVAARLARSLELWRRFTPTLRDGMRA
                     ALEKVASQVKSRDVREIVEKALA"
BASE COUNT          892 a         1517 c         1579 g          954 t
ORIGIN      
        1 gcgcgcagca tgcttgagcg ggcgctggac gcaggtgtgc cgtgcggctg ggtcacgggt
       61 gatgaagtct acggcggcga ccgtcacctg cggctgtggc tcgagtcgcg cggccagtct
      121 ttcgtactgg ccgtcgcgaa gaacgaaccc ctgtggtggc agggtcccga ctacgtacgg
      181 gccgaccgga tcgccgaagc cttgccggca cgggcatggc gacgcctgtc agcaggtgcc
      241 ggcgccaaag gcgagcgcct gtatgactgg gcgttgactc cgctgtggcg tctgcagatc
      301 accacagaag agcgccgctt cgggcattac ctgctggtgc ggcgcagcct ggacgagcaa
      361 cgcgagcatg cgtattacgt cgtatacgcc ccgcgcagca aagccacacg gcaaaccctg
      421 gtcaacgtcg cgggccgcag gtgggaaatc gaaaccggct tcgaggcaac caaaggcgaa
      481 tgcgggctcg atcaatacga ggtacgccaa tggcagggct ggtaccgtca catcacgctg
      541 gcgctgctgg cccatgccgc gctcgtcacc ctgcgggtgc gcgccaaaaa aaacacctga
      601 agattcggtg ccgctcagtg tgcaggagat ccgccggcta ctgtgccgcc tcctatggcg
      661 cggagctcac tccatcgagt acgtcctggc ctggtcggca tggcggcgca ggcaccagta
      721 ccgcgcccag cagtgtcact accggcgccg cggctgcgta cctcctgctt tgttttatct
      781 acggctgtag tactaagttg agccactgct aatttcagaa aagcagcagg ggacgcgcag
      841 agcaggaaga aaagcagaag acgcgaaaaa tacagaagac agaaaaaatg tagaaagcag
      901 caacggaaag caaaaagccc agtcacgagg actgggcttt ttgcttgaat cttgtggtgc
      961 cggaaagagg aatcgaaccc ccgaccttcg cattacgaat gcgctgctct accgtctgag
     1021 ctattccggc atcaggagaa acgagattat agggacttct ttaggaactt ggcaagcccc
     1081 gagggtcaat tttttgtttc gagatggtag cgggtgacgc ggtccacttc gttcttcgaa
     1141 ccgagaaaca ccgccacacg ctcgtgcagg cttttcggct ggatgtccag aatccgcttc
     1201 tcgccgttgg tcgcggcgcc gcctgcctgc tcgacgataa aggccatcgg gttcgcctcg
     1261 tacatcagac gcagcttgcc cggcttgtcc ggcgtgcgtt tgtccgccgg atacatgaag
     1321 atgccgccgc ggttcaggat acggtgcacg tcggcgacca tcgatgcgat ccagcgcatg
     1381 ttgaagtcgc tctggcgcga gccgtccttg ccggccttca attcgccgat gtatttctcg
     1441 accggcggat accaatgacg ctcgttcgaa gcgttgatcg cgtattcacg cgtttcaaca
     1501 ggaatgcgca tctcgctttg cgtgagcacc cacgacccca gttcgcggtc gagggtgaag
     1561 cagttcacgc cgttaccggt ggtcagcacc agcacggttt gcgggccgta caccgcataa
     1621 cccgccgcga cctgctgcgt gcccggttgc aagaacgact gttcggtggg ctgctggccg
     1681 tccgggcagc gcagcaccga gaagatcgtg ccgatcgaca cgttcacgtc gatgttcgac
     1741 gagccgtcga gcggatcgaa caccagcagg tattcgccct tcgggtagtt ggccgggatc
     1801 gggaagaact gctccatttc ctcggaagcc atgcccgcca ggttgccgcc ccattcgttc
     1861 gcttcgagca ggatttcgtt cgacaggatg tcgagcttct tctgcacctc accctggaca
     1921 ttctcgctgc ccgccgtgcc gagcgcatcg cccagcgcgc ccttgctgac gtggtagcta
     1981 atcgccttgc acgcgcgcgc gacgacttcg atcaaaaggc gcaaatcggc cggcaggttg
     2041 ttggtctcgc gctgctgctc gatcaggtac ttcgtgagag tggtacgacg ttgcaaagcc
     2101 attgctgtac tccgggggct agaggaatat ctcgatttta accgctcgcc gtgtcggttc
     2161 cgtgcttttg agacggaagg gtgtggggcc gcgctgcgcg gcgccgcctc aggtggcgca
     2221 attcaggctt tgcggcgcgc cttgatggcc gtgacgttac cggtgaggct ggtggccagc
     2281 gctgcggcgc gggcgtgttc gggcgagact ttcggcattt tcggccggtc cgccttaggc
     2341 attggctcga tctcgcgttc gatctgcgcg ccggctgccg cagtggcgac gcgcaggtcg
     2401 tagccgatct gcaggttcag ccagaactgt gcgctcgcac cgaagtaacg ctcgagccgc
     2461 aacgcggtgt cagccgagat ggcgcgtttg ccgcgcacga tgtcgttgat gcgcggcgcc
     2521 ggtacgcgca gcgcaagcgc cagcgcattg acggacatgc cgagcggttc gaggaactcg
     2581 ctgcgcagaa tcccccccgg gtggatctcc gggatgcttt cgccggtgcc gatgtcggag
     2641 aaatcgatgt tgtccagatc ggagcgtttg atgaccatgg cggtctccca ataatcagtg
     2701 atagtcgaca atttcgacat tcagcgcctc cccgtcgaca aaatgaaaac agatgcgcca
     2761 ctgcgcgttg atacgaatgc tccactgccc gctgcgctgc ccttgcaagg cttcgagccg
     2821 attgcccggt ggcgcgtgca ggctgcggat ggactcagcg gcgtcgatca gcagcagctt
     2881 gcggcgtgcg agcgcctgca tgtaaagcgg cagtgaatgc acgaattttc cctcaaagat
     2941 tgccgccgta gcttcgtcgc cgaatgtcgt gatcaaggga tgatataacg tggtgcgtta
     3001 aacgtaaacc gttaaatgaa cgcgtttact ttcacccagc cgagccgata cgaacagtcg
     3061 tccagatggc taacgtcggg gtccgcacgc tgcctcacgg cgaccttcag gacgaaaaaa
     3121 aaccggcaac gcgtgccggc ttttcgatgc gggaagcgct ttcaggtgcg cgtcacgcca
     3181 acgccttctc cacgatctcg cgcacgtcgc gcgatttcac ctgcgaggcc actttctcca
     3241 gcgccgcgcg catgccgtca cgcagcgtcg gcgtaaaccg gcgccatagt tccagcgagc
     3301 gggcgaggcg ggcggccact tgcggattga tggcgtcgag cgcgatcacc tgctcggccc
     3361 agaaggcgta acccgagccg tcttcggcat ggaactgcga gggattcgcc gcacagaagc
     3421 tgaaaatcag cgagcgcgcg cggttcgggt tcttcaggtt gaacgccggg tgcgccatca
     3481 gcttgcgcac gatctcgatc accggacgct gcgcgctgcc gcgttgcgtg gcttgcaggg
     3541 caaaccactt gtcgatgacg agcggctctt tttcgaagcg ccgataaaaa tcgtccagcg
     3601 cctgccgcgc ctgaacgctg ccgccattcg ccgccgctgc gttgagcaag gccgagagcg
     3661 ccgccgagcg gtcggtcatg ttgttggcgg cgtcatactg cgcggacgcg aggcgtacgg
     3721 cctcagccgg atcgtccagc tccgcgagat acgacaacgc gagattcttc agcgcacgat
     3781 gaccggaggc ctccggtgtc gcttcgtaag tacccggggt gcggtgcttt tcgtacacgg
     3841 cgagccagtc cttcctgagc gcattcgcga ggcgtttgcg cacgaactgc cgtgcggcat
     3901 gaacggccgc cggattcgat tcggccatct gttcggccag atacgcttcc gacggcagca
     3961 tcagcgccag ttcgcggaac gagggcgaga gcgattcgtc ggtcagcacg cgggcgaacg
     4021 cggcgaccac cgagtcctcg agttgcagcg gcacgccggt ggcggcgcgc ccggcaagcg
     4081 tcagcagctc gcgcgtggcg agccgttggc cggcttccca gcggttgaac gggtcgctgt
     4141 cgtgcgcgag caggaacgcc agttgatcgg ccgaatagtc gtattcgacg atcaccggcg
     4201 ccgagaaatt acgcagcagc gaaggcagtg gttcctgtgc gacgttgacg aacgtgaagg
     4261 tctgttcgct ctgcgtgaat tcgagcacgc gcgtggtcgc cttgccggcc tgcgcttcgc
     4321 cttccagttg cagcggcaga tccgtgccgt tcttgccgat caagccgatc gcgaacggaa
     4381 tcagcagcgg ccccttttgc gtttcgcgcg cggtggctgt gccttcgcca taaccttgcg
     4441 tgagcgtcat gctgtagcgt ttttgcgcgg cgtcgtacct ggtgcgtacc gagacgcgcg
     4501 gcgtgccggc ctggctatac cagcgctcga attgcgcgag atcgcggccg ttcgcatccg
     4561 cgagcgcgtg acggaaatcg tcgcaggtca cggcctgccc gtcatggcga ttgaagtaca
     4621 ggtccatgcc cttgcgaaag ccgtcgcggc cgaacagcgt ctgatacatc cgcacgactt
     4681 cggagccctt ctcatagacc gtcatcgtgt agaagttgtt gatctcgacg tagctttccg
     4741 gacgcaccgg gtgcgccatc ggacccgcgt cttcggcgaa ctgcatctgg cgcagcacgc
     4801 gcacgtcttc gatacgcttg gtggcgcggg cggcttcgtc ggtggcgccg ccggccatgt
     4861 cggccgagaa ttcctggtcg cggaacaccg tcagaccttc tttcaggctc agctggaacc
     4921 agtcgcggca ggtcacgcgg tt
//
LOCUS       DATASH010000450         8887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5610526,
            whole genome shotgun sequence.
ACCESSION   DATASH010000450 DATASH010000000
VERSION     DATASH010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 8887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8887
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5610526"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2780)
                     /locus_tag="VHV99_12465"
     CDS             complement(<1..2780)
                     /locus_tag="VHV99_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013342846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HEX3636282.1"
                     /translation="MHSIDAVAARRALSRPFVLFFFALLSWFVTPHAQAGACDSRYAA
                     AGAYPADPQCPLKAATADFGGMGGHVCGDPTVIAAYCSGPSVTDTTEPASPEGSMPDS
                     GQNCSPDASAGCGNSTSGADPVNLYTGQFYFFAHDLTLADTITLDLTRVYRSGAYNSS
                     GRPLTGAFGAGAGLTFDTILAMSADRQRFELRLATGIRVPFTPRAGSGGKGWDDLTSP
                     GEYYSARIDAAGSAGMTLSLRDGRIQQFTMINGIYRLARLQDRNGNTVMIARDSKTGA
                     ITGITSPNGRVLTFTSITGSRGTPLVSRVSDSLNRQVSYRYDSQDRLTEVTDAGGGVC
                     KYGWDSKSRLVTVTDPEGNVQVTNTYNDNDRVVSQQLADNSSFGFAYGLSDGKVTQTE
                     VTDRRGSIRSLEFDVNGRVVRNTYPAGQPAQQVQTYTYDATGRVTNFTSTDRLYAYTY
                     DGNGNRTSEADQYGTLGTRTFDSYSQLLTEAQPGDAQRGVATVYTYDPKGNLLTITDR
                     MGNRTTQTNDSQGRPLTVTDALKGVTKFTWTGADLTSVTDALNRTTQYTTDAAGRVTA
                     VQDPLGNKTTRTLDALDRTTDVTDALGGITHFTWDRARHLLSQADPKGVTTRYTYNTI
                     GRPVSKTDPQGRSEIYTWTPAGQLQSVTDRKGQVTGYTYDEAGRLKRIAFQAAAGAPS
                     TRRWNYVWDNTMDRLQGLDDYALVEQDGIETRTGTTIFYYDSVTGKFVRTFEWPAQSG
                     TWSYRYNAVTRELDGINMFFATVTYSRDAEHRVTQIQSVENDEAPRQFGYGYDALGRL
                     AQATLANGITANYAWDAASQLTGITYKRSDGGVLGDLTYGYDLAGRRTKMGGSLARAD
                     LPQPVSDTQYNGANQLTRWSGKTFTYDTNGNVIDDGLNQYSWNLQGLLGGIMGKTTAA
                     FSYDASGHRRNMT"
     gene            complement(2825..3088)
                     /locus_tag="VHV99_12470"
     CDS             complement(2825..3088)
                     /locus_tag="VHV99_12470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat domain-containing protein"
                     /protein_id="HEX3636283.1"
                     /translation="MASLITLCTLARADEITYGYDALGRLTSVSVAGATAYYDYDAAG
                     NITAIRRHGALSSTLPMNIDGPSRASEGAILPASPALAQTRMR"
     gene            complement(3571..4185)
                     /locus_tag="VHV99_12475"
     CDS             complement(3571..4185)
                     /locus_tag="VHV99_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX3636284.1"
                     /translation="MMKLIGSLGSPFVRKARIVLADKKIEYQLVPENVWAPDTTIHTF
                     NPLGKVPCLVMEDGEAVFDSRVICEYVDTLSPVGKLIPPSGRERVEVRCWEALADGIL
                     DAAVLIRLEGTQRTPEQRVDTWVARQQRKIDEGLVAMSQGLGSKAWCTGNHYTLADIA
                     VGCALGYLDFRMPELNWRETYPNLDKHFQKLSLRQSFVDTVPAD"
     gene            4526..5932
                     /locus_tag="VHV99_12480"
     CDS             4526..5932
                     /locus_tag="VHV99_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HEX3636285.1"
                     /translation="MNQPTEPTIAIDVYRTRRERVLAALRAAGGGVAIVPTAPEKLRN
                     RDADYPYRHDSYFYYLTGFTEPEALLVLDASAATGEPASILFCREKNVERETWEGFRF
                     GPEGAREAFGFDAAFAVSEVDTQLPRILADKPSLHYALGTSAQLDNQVRGWLDAVRAQ
                     GRSGVAAPNLARDLIPLLDDMRLIKDHHELAIMRRAGQISAQAHRRAMAACHPGVREY
                     ELEAELLYTFRKFGAQAPAYTSIVAAGANACVLHYPAGNAIAQDGDLILIDAACELDG
                     YASDITRTFPASGRFTPAQRELYDIVLAAQQAAVDATRVGATFDDPHQAAVRVLSQGL
                     LDTGIVERTKLASVDDVIAERAYAPFYMHRTGHWLGMDVHDVGDYRERGAPRDEAGAL
                     PWRTLQPGMTLTIEPGLYIRPAEGVPERYWNIGIRIEDDAIVTPAGCELITRDVPVAA
                     DEIEALMQQARAAQEAKQ"
     gene            5977..7224
                     /locus_tag="VHV99_12485"
     CDS             5977..7224
                     /locus_tag="VHV99_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiH/UbiF/VisC/COQ6 family ubiquinone
                     biosynthesis hydroxylase"
                     /protein_id="HEX3636286.1"
                     /translation="MNDVPQSTVILRPAVSDHPFDFDVTIVGAGPVGLALAGWLARRS
                     ATQALKIALIDAREPEDSIADPRAIAVSHGSRMILEPLRWPADSTAIQRIHVSQRGHF
                     GRTLIDHSEHGLPALGYVLRYGSIVHGLAEAVHATSVHWFRSTSASAPTQERDGVTLP
                     IETAGVTRHLRTRILVNAEGGLFGDQRSGKRAGSKPGSRTEGGTRDYWQTALVGTVSV
                     SAPQPHVAWERFTSQGPIALLPMGGVRGADYALVWCCAPDEAARRSQLSNDEFLRELG
                     AAFGSRMGRFTQIKGRASFPLGLNAVDTLVNGHVVAIGNAAQTLHPVAGQGLNLGLRD
                     AHALADALSAEGPTPLALATFAQRRALDRRLTIGATDTLARLFTVDFPPLAALRGLAL
                     TALEFVPPVKTALARQMMFGQRR"
     gene            7476..8537
                     /gene="dusB"
                     /locus_tag="VHV99_12490"
     CDS             7476..8537
                     /gene="dusB"
                     /locus_tag="VHV99_12490"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006401674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEX3636287.1"
                     /translation="MPTLGSHNLRNNLFVAPMAGVTDRPFRQLCKRLGAGYAVSEMVA
                     SNAQLWKSEKTMRRANHTGEVEPIAVQIAGADPAMMAEAARYNVANGAQIIDINMGCP
                     AKKVCNVAAGSALLQNEPLVQRIVEAVVGAVGVGPDAVPVTLKIRTGWDRDNKNALSV
                     ARLAEAAGISMLTVHGRTRADLYHGDAEYETIAAVKAAVRIPVVANGDITSPQKAREV
                     LAATGADAIMIGRAAQGRPWLFREIEHFLQTGELLPPPRIDEIQQVMNEHLEDHYAFY
                     GEFTGVRTARKHIGWYTRGLSGASVFRHRMNTLDTTREQLLAVNEFFDAQKALSDRLV
                     YVDETPENQGEDPTDRLAA"
     gene            8534..8767
                     /locus_tag="VHV99_12495"
     CDS             8534..8767
                     /locus_tag="VHV99_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013436270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HEX3636288.1"
                     /translation="MSKKNIEQSVRDSLGMYFQDLDGSNPHDVYDMVISCVEKPLLEV
                     VLEQAGGNQSLAAEYLGINRNTLRKKLQQHGLL"
BASE COUNT         1674 a         2895 c         2677 g         1641 t
ORIGIN      
        1 gtcatgttcc tgcgatgacc cgatgcgtcg tagctgaacg ccgccgtggt cttgcccatg
       61 atcccgccaa gcaagccctg caggttccag ctgtactggt tcagtccatc gtcgatgacg
      121 ttgccgttcg tgtcataagt gaacgttttt cctgaccagc gcgtgagctg attcgctccg
      181 ttgtactggg tatcgcttac cggctgcggc aggtccgccc tcgccagact gccgcccatc
      241 ttcgtccgcc ggcccgccag gtcataaccg taggtcaggt cacccagcac gccaccgtcg
      301 gaccttttgt atgtgatgcc cgtcagctgg ctcgccgcat cccacgcata gttcgccgtg
      361 atgccgttcg cgagcgtcgc ctgtgcaaga cggcccaacg catcatagcc gtagccgaac
      421 tggcgcggcg cctcatcgtt ctccaccgac tggatttgcg tgacccggtg ttccgcgtca
      481 cggctatagg tcaccgtcgc gaagaacatg ttgatgccgt ccagttcgcg cgttacagcg
      541 ttgtaccggt acgaccaggt tccagactgc gcgggccact caaacgtgcg cacgaacttt
      601 cccgtcacgc tgtcgtagta gaagattgtg gtcccagtgc gcgtttcaat accgtcctgc
      661 tcgaccaggg cgtaatcgtc cagaccctgg agacggtcca tcgtgttgtc ccacacgtag
      721 ttccaccgac gcgtggacgg agcgcccgcc gcagcctgga aggcaattct cttcagcctg
      781 ccagcttcgt cgtacgtgta acccgtcacc tggcccttac gatcggtcac gctctgcagt
      841 tgccccgccg gcgtccacgt atagatctcg ctgcggccct gcgggtccgt tttactcact
      901 ggacggccaa tcgtgttgta cgtgtagcgc gtcgtgacgc ccttcggatc ggcctgactc
      961 agcaggtggc gggcgcggtc ccacgtgaag tgcgttattc cgcctaatgc gtcggtaacg
     1021 tcggtcgttc ggtcaagcgc gtcaagtgtg cgcgtagttt tgttgccgag tggatcctgc
     1081 accgccgtca ctcgacccgc tgcgtcggtg gtgtactgcg tggtgcgatt cagcgcatcg
     1141 gtcacgcttg tcagatccgc accggtccag gtgaacttcg tcacgccctt cagcgcgtcg
     1201 gtcaccgtca gcgggcggcc ctggctgtcg ttcgtctgcg tggtgcggtt gcccatccgg
     1261 tcagtaatcg tcagcaggtt gcccttcgga tcgtacgtgt agacagtcgc gacgccgcgc
     1321 tgcgcatccc ctggctgcgc ctcggtcagc aactggctgt agctgtcgaa cgtacgggtg
     1381 cccagcgtgc cgtactgatc ggcctcgctc gtgcggttgc cgttcccgtc gtaggtatag
     1441 gcgtacagac gatcagtcga cgtgaagttc gtcacccggc ccgtcgcatc gtatgtgtaa
     1501 gtctgtacct gctgcgccgg ctggccggcg ggataggtgt tacgcaccac ccgcccgttc
     1561 acatcgaact cgaggctccg tatgctgccg cgccggtcgg tcacttccgt ctgtgtgacc
     1621 ttgccgtcgg acagcccgta cgcaaagccg aagctgctgt tgtcggccag ctgctgcgag
     1681 acgacgcggt cgttgtcgtt gtaagtgttg gtgacttgaa cgttgccttc ggggtccgtg
     1741 accgtcacca accgtgattt gctatcccag ccgtatttgc atacgccgcc gcccgcgtcc
     1801 gtcacctcgg tgaggcggtc ctggctgtcg taccggtaac tcacctggcg gttcagcgaa
     1861 tcactcacgc gtgagaccaa cggcgtgcca cggcttccag tgatcgacgt gaacgtcaac
     1921 acgcggccat tcgggctggt gatgccggtg atcgcgccgg tcttgctatc gcgggcgatc
     1981 atgacggtgt tgccgttgcg gtcctgcagg cgagcgagcc ggtagatgcc gttgatcatc
     2041 gtgaactgct gtatccggcc atcacgcaag ctcaacgtca tgccggcgct gcccgcagca
     2101 tcgatacgcg cactgtaata ctcgccaggg ctcgtcaggt catcccagcc tttgccaccg
     2161 ctgcccgcgc gtggtgtgaa cggcacacga ataccggtcg cgagccgtag ctcgaagcgc
     2221 tgacggtccg cactcatcgc gaggatcgta tcgaaagtca ggcccgcccc tgcgccgaat
     2281 gcgccagtca aaggccggcc tgaactgttg taggctccgc tgcgatagac ccgcgtcaga
     2341 tccagagtga tcgtgtcggc aagcgtgaga tcgtgtgcaa agaaatagaa ctgaccggtg
     2401 tacagattca ccgggtccgc gcccgacgtg ctgttgccgc acccagcgct tgcatcgggc
     2461 gaacaattct gaccggagtc gggcatgctg ccctcgggcg aagcgggctc ggtggtgtcg
     2521 gtaaccgacg gtccgctgca ataggccgcg atcacggttg gatcgccgca tacgtgacct
     2581 cccatgccac caaagtctgc cgtcgcggct ttgagtgggc attgtggatc agccgggtaa
     2641 gcccccgcag ccgcataacg gctgtcgcat gcaccggctt gcgcgtgcgg cgtgacgaac
     2701 cacgagagca gcgcgaagaa aaacagaacg aacgggcgcg atagcgcgcg ccgcgcggca
     2761 acggcatcga tagagtgcat gaatgtttgg atgagactgg aaaacggatc gaccgcggcc
     2821 gcatttagcg catgcgagtc tgggccaagg caggcgatgc tggcaatatc gcgccttcag
     2881 aagcgcgaga aggaccatcg atattcatcg gcaaagtcga agacaacgcc ccgtgccggc
     2941 ggatcgccgt gatattgcct gccgcgtcat agtcgtaata agcggtggcg cccgcaacag
     3001 agactgatgt gagacggccc agcgcgtcgt agccgtaagt gatctcatct gcacgggcga
     3061 gtgtgcagag tgtgataagc gaagccacgg cgccgcacct gacataccgt cgaataccgc
     3121 ttgtatatcc cgcaactgtg cctgtgggtc taatcatcga cactgcctcc acagacgttt
     3181 atgtattaaa aaatatttga gcgagaatac aggatttcag aatgaataaa aagtcagata
     3241 aatttgactt cacataggaa ttcggagcct ccagtgtagg aatagttttc tcatgattgg
     3301 cgacctatga gaagaccatg aagaagcgcg caccgccgct tcgcgatggc cacacatcac
     3361 cggataaaga ctcatatacg tgcgttgcac aaccattacg acgcgacaga caaaaaatac
     3421 gcctgacaaa caggcgcatt ttctataaaa cataatcttt aatttaatat tgcggaattc
     3481 agaattacat cattttcaat ccagcgaaaa tgacaagcaa agttattcca gacagacaac
     3541 cgaacgacta aacactcacg agtacctcac tcaatccgcc ggcaccgtat caacaaacga
     3601 ctgacgcaac gaaagcttct ggaaatgttt atccagattc ggatacgtct cgcgccagtt
     3661 caactcaggc atgcggaaat cgaggtagcc caatgcgcaa cccacggcga tatccgcgag
     3721 cgtgtagtga ttgcccgtgc accacgcctt gctgcccaag ccctgcgaca ttgcgaccag
     3781 gccttcgtcg atcttgcgtt gctgccgcgc gacccacgtg tccacgcgct gctcgggcgt
     3841 gcgctgggtg ccttcgaggc gaatcagcac cgcggcatca aggatgccgt cggcaagcgc
     3901 ctcccagcaa cgcacttcga cgcgctcgcg gcccgacggc ggaatcagct tgccgaccgg
     3961 cgacagcgta tccacgtatt cgcagatcac ccgcgagtcg aacacagctt cgccgtcttc
     4021 catcacgagg cacggcacct tgccgagcgg attgaaggtg tgaatcgtgg tatccggcgc
     4081 ccagacgttc tcgggcacga gttgatattc gatcttcttg tcggccagaa cgatccgcgc
     4141 tttacgcacg aacggactgc cgagcgaacc gatgagtttc atcatgacca tctgcctttt
     4201 cttgaattcg gcgaaagtat aagtggtcgg gggctttccc gagcgcagcg aactcgcttc
     4261 gcaagccgca tgaatgctcg cagtgaatgg attgcaacat tgccgcaacg ctcacgcgcc
     4321 gtctacccgg ccgaacggct aacgccacca acgcgggcgg cacgcctcat tgcacgcacc
     4381 cctcaaatgg ccggccggca tacggcgccg gacgcccacg gcggccaaac ggtcttgcgc
     4441 gcggcgcgaa ccgatcagcg cggcggccct tgtcccacag tatccgcgca aaataagtcg
     4501 ccgcattggg cgctacaatc gcaacatgaa ccagccgacc gaacccacca tcgccatcga
     4561 cgtctaccgc acgcgccgcg agcgcgtact cgccgcgctg cgcgcagcgg gcggcggcgt
     4621 cgccatcgtc cccaccgctc cggaaaagct gcgcaaccgg gacgccgact acccctaccg
     4681 gcacgatagc tatttctact atctgacggg cttcaccgaa cccgaggcgt tgctggtgct
     4741 cgacgcaagc gccgcgacgg gcgagccggc ttcgatcctg ttctgccgtg agaagaatgt
     4801 cgagcgcgag acgtgggaag gtttccgttt cgggccagag ggcgcacgcg aggcgttcgg
     4861 cttcgacgct gccttcgccg tgagcgaagt cgatacgcaa ctgccacgca ttctggccga
     4921 caagccgtcg ctgcattacg cgctcggcac ctcggcgcaa ctggacaacc aggtgcgcgg
     4981 ctggctcgac gccgtgcgtg cgcagggccg cagcggcgtc gccgctccca acctcgcgcg
     5041 cgatctgatc ccgctgctcg acgacatgcg gctcatcaag gaccaccacg aactcgccat
     5101 catgcgccgc gccggacaga tttccgcgca agcgcaccgg cgtgcaatgg ctgcctgcca
     5161 cccaggcgtg cgcgagtacg agctcgaagc cgagctgctc tacacgttcc gcaaattcgg
     5221 cgcgcaggcg ccggcttata cctcgatcgt cgccgcgggc gcaaatgcct gcgtgctgca
     5281 ctacccggcc ggcaacgcga ttgcccagga cggcgacctg atcctgatcg acgccgcctg
     5341 cgaactcgac ggctacgcgt ccgacatcac ccgcaccttc ccggctagcg gccgcttcac
     5401 accggcgcag cgcgagctgt acgacatcgt gctggccgcg cagcaggctg ccgtcgacgc
     5461 cacccgcgtc ggcgccacct tcgacgaccc gcaccaggcc gccgtgcgcg tgctgtcgca
     5521 aggtctgctc gacaccggca tcgtcgagcg cacgaagttg gcctcggttg acgacgtaat
     5581 cgccgagcgc gcctacgcgc cgttctatat gcaccgtacc ggccactggc tcggcatgga
     5641 cgtgcacgat gtcggcgact accgcgaacg cggcgcaccg cgcgacgaag cgggcgcgct
     5701 gccgtggcgc acgctgcagc cagggatgac gctgaccatc gagcccggtc tgtatatccg
     5761 cccggccgaa ggcgtgcccg agcgctactg gaacatcggc atccgcatcg aggacgacgc
     5821 gatcgtcacg ccggccggct gcgagctgat cacacgcgac gtgccggtgg cggccgacga
     5881 gatcgaggcg ctgatgcagc aagcccgcgc ggcgcaggaa gcaaagcagt aagccaggca
     5941 gtcacgcaag gcagtaaccc aaggaagcaa cccgcaatga acgacgttcc ccagtcgacg
     6001 gtaattctga gaccggctgt atccgatcat cccttcgatt tcgatgtgac gatcgtcggc
     6061 gccgggccgg tcgggctggc ccttgccggc tggctggcgc gccgcagcgc gacgcaggcg
     6121 ctgaagatcg cgctgatcga cgcgcgcgag ccggaagatt cgattgccga tccacgcgca
     6181 atcgcggtgt cgcacggcag ccggatgatt ctcgaaccgc tgcgctggcc cgcggactcg
     6241 accgcgattc agcgcattca cgtatcgcag cgcggtcatt tcggccgcac gctgatcgac
     6301 cacagcgagc acggactgcc ggcgctcggc tacgtgctgc gttatggttc gatcgtccat
     6361 ggcctcgccg aagcggttca cgcaacgtct gtgcactggt tccgctcgac ctccgcgtcg
     6421 gcgccgactc aggaacgcga cggcgtcacg ctgccgatcg aaaccgcggg cgtcacgcgt
     6481 catttgcgta cgcgaattct ggtgaatgcc gaaggcggtc tgttcggcga ccagaggtca
     6541 ggcaagcgcg ccggtagcaa gccgggcagc cgcaccgaag gcggcacgcg cgattactgg
     6601 caaaccgcat tggtcggcac ggtcagcgtg tcggcgccgc aaccgcacgt ggcgtgggag
     6661 cgcttcacgt cgcaagggcc gatcgcgctg ctgccgatgg gcggtgtgcg cggcgccgat
     6721 tacgcgctgg tctggtgctg cgcgccggac gaagccgcac gccgctcaca actctccaac
     6781 gacgaattcc tgcgtgaact cggcgccgca ttcggcagcc gcatgggccg cttcacgcag
     6841 atcaaaggac gcgcgtcttt tccgttggga ctgaacgcgg tcgataccct cgtgaacggc
     6901 catgtggttg cgatcgggaa cgcagcgcag acactgcatc cggtggcagg tcagggtctg
     6961 aatctcggtc tgcgcgacgc gcatgcgctc gccgatgcct tgtcggcgga aggtcccact
     7021 ccgctcgcgc tggctacgtt cgcgcaacgc cgcgcgctgg accggcgcct gacgatcggc
     7081 gccaccgaca cgctcgcgcg ccttttcacc gtcgacttcc cgccgctcgc cgccttgcgc
     7141 ggtctcgccc tcaccgcgct ggaattcgtg ccgccggtga aaaccgcgct ggcgcgacaa
     7201 atgatgttcg gtcagcgccg ctgaacgcgc cgcccgctcg cgtcccacag ccgttgcagc
     7261 ttttttggac agcgcacgcc gagccgcgcg agcgctgccg gttttatcaa tccggccact
     7321 ttcatagaag atttaatgag ttacgactga cgcaagtcgg gtgtcggcta tctgttaacg
     7381 ctaaaatagc ggttttcccc ttgcatttcg cgaatgctcc gattgacaga atctgaaaag
     7441 aaactgcagc aatcggtcgc aggcgcgtcc acgtcatgcc tactctcggc tcccacaatc
     7501 tgcgtaataa cctgttcgtc gcccccatgg ccggcgtgac cgaccggccg ttccggcaat
     7561 tgtgcaaacg gctgggcgcg ggctatgcgg tgtcggaaat ggtcgcctcg aacgcgcagt
     7621 tgtggaaaag cgaaaagacc atgcgccgtg ccaaccacac gggcgaggtc gagccgatcg
     7681 ccgtgcagat cgccggcgcc gatccggcca tgatggccga agccgctcgc tacaatgtgg
     7741 cgaacggtgc gcagattatc gacatcaaca tgggctgccc cgccaagaag gtgtgcaacg
     7801 tggccgcggg ttctgccttg ctgcagaacg aaccgctggt gcagcgcatc gtcgaggcgg
     7861 tggtaggcgc ggtgggcgtg gggcccgacg cggtgcccgt cacgctgaag atccgtaccg
     7921 gctgggatcg cgacaacaag aatgcgttgt ctgtcgcgcg gctggctgaa gcggccggca
     7981 tttccatgct gaccgtgcac ggccgcaccc gcgccgacct gtatcacggc gacgccgaat
     8041 acgagactat cgctgccgtc aaagcggcgg tgcgcattcc ggtggtcgcc aatggcgaca
     8101 ttacgtcgcc gcaaaaagcc cgcgaggtgc tcgccgccac cggcgccgac gccatcatga
     8161 tcggccgcgc cgcgcagggg cgcccgtggc tgttccgcga gatagagcat ttcctgcaaa
     8221 cgggcgagtt gctgccgccg ccgcgcatcg acgaaatcca gcaggtgatg aacgagcatc
     8281 tcgaagatca ctacgcgttc tacggcgaat ttacaggtgt gcgcactgcg cgcaagcaca
     8341 tcggctggta cactcgcggc ctttccggcg ccagcgtgtt ccggcatcgc atgaatacgc
     8401 tggacaccac gcgcgaacaa ctcctcgccg tcaacgagtt cttcgacgca caaaaggcgc
     8461 tctccgaccg cctggtctac gtcgacgaaa cgcccgagaa ccagggcgag gaccctaccg
     8521 accgactggc agcatgagca agaagaatat cgaacaatcc gtgcgcgaca gcctgggcat
     8581 gtacttccag gatctcgacg gctccaatcc gcacgatgtc tatgacatgg tgatttcgtg
     8641 cgtggaaaaa cccctgctcg aggtggtgct cgagcaggcc ggcggcaatc agtcgctggc
     8701 cgccgaatac ctcggcatca accgcaacac gttgcgcaag aagctgcaac aacacggttt
     8761 gttgtagcga ttggtcgtat cttcgcgccc cgccacgttt ctcttcggct tttcgtcttc
     8821 atcatgatca agcaagcgct catctccgtt tccgacaagt ccggcatcgt cgacttcgcg
     8881 aagtcgc
//
LOCUS       DATASH010000451         3246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4026497,
            whole genome shotgun sequence.
ACCESSION   DATASH010000451 DATASH010000000
VERSION     DATASH010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4026497"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VHV99_12500"
     CDS             <1..241
                     /locus_tag="VHV99_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX3636289.1"
                     /translation="QYCLRVAHHHQAKTLVITDSQLSPLARYATTQLYVKEGSAFAFR
                     SLTSTICLCQALFIALAYKLELNVEESKDTGGYDD"
     gene            234..1250
                     /gene="iolG"
                     /locus_tag="VHV99_12505"
     CDS             234..1250
                     /gene="iolG"
                     /locus_tag="VHV99_12505"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HEX3636290.1"
                     /translation="MTEAVKTIDVAVFGAGRIGKIHAANLARQPGVRLKYVVDVNREA
                     AAALAAEHGAQVADIDGAMGDASIGATVICSSTDTHADLITKSAAQKKHVFCEKPVDL
                     TLERARACAEAVGHAGVVCMIGFQRRFDPTFSALKARIDAGEIGTPEMLVVTSRDPGA
                     PPVEYIKHSGGIFKDMLIHDFDIFRWILDDEADTLHATGSCLSDPAIAEAGDIDSTAV
                     TIRTKHGRLCQINTARRAAYGYDQRFEVLGSTGMLQAGNVRPTEVTAYSKTEVSSDVP
                     EAFFLERYRAAYALEIAHFFDAVTRGKPVRTTVADGLKALELAEAATRSWREGRAIKL
                     GEAL"
     gene            1250..2293
                     /locus_tag="VHV99_12510"
     CDS             1250..2293
                     /locus_tag="VHV99_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3636291.1"
                     /translation="MKASAGPLRMGVIGLGRLGKRHAENLAYRVPGASLVAACSPLEE
                     ERAWARDMLPEPRLYDDYADLLADPEVDAVWLVTPSSLHAQQIIDALRAGKHVFCEKP
                     LSLDLAECERVLAEAARCPHLQATIGFMRRFDPSYQDAFDKIQAGAIGRPFLVRSQTT
                     DKNDDDGFFVRFAASSGGIFLDCTVHDIDVARWLLGRPRAKRVFAAGAVALHEGLREF
                     GDVDNGVAICEFEDGKLAMFYASRTQAHGNDTHSEVIGTAGAVAIGRNPRANRVEIYD
                     AAGIRNECTSSFFDRFEEAFLFEARGFVAAVQAGAAGAGRYAGASLADALEATRIGVA
                     LRQSLLRGEAVVL"
BASE COUNT          545 a          883 c         1092 g          726 t
ORIGIN      
        1 gcagtattgc ctgcgcgtcg cgcatcatca tcaggcgaag accttggtta ttacggatag
       61 ccaactttct ccgctagcgc gttatgccac cactcaactg tatgtgaaag agggcagcgc
      121 attcgcgttc cgctcgctga ccagcacgat ctgtttgtgc caggcgttgt tcatcgcgct
      181 cgcgtacaag ctcgaactga acgttgaaga atccaaagac acgggaggat acgatgactg
      241 aggctgtaaa gacgatcgac gtggcggtat tcggcgcagg gcgtatcggc aagattcatg
      301 cggccaatct ggcgcgccag cccggcgtgc ggctcaaata tgtggtcgat gtgaatcgcg
      361 aggcggcagc ggcactcgct gccgagcacg gcgcgcaggt tgcggatatc gacggcgcga
      421 tgggcgatgc gtcgatcggc gcgacggtga tctgttcgag cacggacacg cacgcggatc
      481 tgatcacgaa atcggccgcg cagaagaagc atgtgttctg tgagaaaccg gtggacctga
      541 ctttggagcg ggcgcgtgcc tgtgcagagg ctgtcgggca tgctggcgtg gtctgcatga
      601 tcggctttca gcgccgtttc gacccgacgt tttccgcctt gaaagcgcgc atcgatgcgg
      661 gcgaaatcgg cacgccggaa atgctggtgg tgacgagccg cgatccgggc gcgccgcccg
      721 tcgagtacat caagcactcg ggcggcattt tcaaggacat gctgattcac gacttcgaca
      781 tcttccgctg gatactcgac gacgaagccg acaccttgca tgctaccggc agttgtcttt
      841 cggacccggc gattgccgaa gccggcgata tcgattcgac cgcggtgacg atccgcacga
      901 agcacggccg tctatgtcag atcaacaccg cgcggcgcgc ggcttatggt tacgaccagc
      961 gtttcgaagt gctcggcagc accggtatgt tgcaggcggg taatgtgcgt cccaccgagg
     1021 tcaccgcgta ttcgaaaacc gaagtctcga gcgacgtgcc cgaggcgttt ttcctcgaac
     1081 ggtatcgcgc ggcttatgca ctggaaatcg cgcatttctt cgacgcggtc acgcgcggca
     1141 agccggtgcg tactactgtc gcggacggcc tgaaagcgct cgaacttgcc gaagcggcca
     1201 cgcgctcgtg gcgcgagggt cgcgcgataa agctcggcga ggcattgtga tgaaggcgtc
     1261 ggcggggccg cttcggatgg gcgtgatcgg cttggggcgg ttgggcaagc ggcacgcgga
     1321 aaatctggcg taccgcgtgc cgggggcatc gctcgtggcc gcttgcagtc ccttggaaga
     1381 ggagcgcgcc tgggcccgag atatgttgcc tgaaccgcgt ctatatgacg actatgcgga
     1441 cctgctggcc gatcccgaag tggacgcggt gtggcttgtc acgccgtcgt cgttgcacgc
     1501 gcaacagatt atcgatgcgt tgcgcgccgg caaacatgtg ttctgtgaga agccgctatc
     1561 actggatctt gccgagtgcg agcgcgtatt ggccgaagcc gcgcgctgtc cgcatctgca
     1621 ggcgaccatc ggcttcatgc ggcgtttcga tccgagctat caggatgcgt ttgacaagat
     1681 ccaggctggc gcgattgggc ggccttttct tgtgcgttcg cagaccactg ataagaacga
     1741 tgacgatggc tttttcgtgc gtttcgcggc gagctccggc gggatttttc tggactgcac
     1801 cgtgcatgac atcgatgtcg cgcgctggct gcttggcagg ccgcgggcca aacgcgtgtt
     1861 tgccgcgggg gcggttgcgt tgcacgaggg tttgcgggaa tttggcgatg ttgataacgg
     1921 tgtggcgatt tgtgagttcg aggatgggaa gctcgcgatg ttctatgcgt cgcgcacgca
     1981 ggcgcatggg aatgatacgc atagcgaggt aattggtacg gcgggtgcgg ttgctattgg
     2041 gcgtaatccg cgtgcgaatc gcgttgagat ttatgatgcc gccggcattc gtaatgaatg
     2101 tacgtcgagt ttttttgatc ggttcgagga agcgtttttg tttgaggcgc gggggtttgt
     2161 tgccgctgtg caagcgggtg ccgctggggc tggcaggtat gccggggcct cgctggccga
     2221 tgcgctcgag gccacgcgga ttggcgtggc tttgcggcag tcgttgttga ggggggaggc
     2281 tgttgttttg tagttgacgg cctgcggcgc tggggggagg cggggaggtc ggtctttgct
     2341 ggagtttttt tgcctgcgcg gcgctttttg tctgggcttt tatggtgttg ggctttcctt
     2401 gatctgggtt tttggccttt ccttgaattg atagtggtct attagcgtcg cccctgtgcg
     2461 gggcgggcac ctacttttct ttgcctgccg caaagaaaag taggcaaaag aaagcggctc
     2521 acaccgctag ctcataagcg ggtcccccgc acagtgacgg tagtggtgca tctggaatct
     2581 gtgctcccgc acatttagcg tgagtgacaa ggccgtcata cttcccgcct cgcactgcgt
     2641 gctcgccgga aggggacatc gggaagaccg gcggcttcgc ttgggactcg tgtcagcaag
     2701 cgggtcgctc gcaagtttcg gcagtggtgc atctggaatc tgtgccctcg cgcattcggc
     2761 gtgagtgaca aggccgtcat attttccgcc tcgcactgcc tgctcgccgg aacaccccac
     2821 cggaagaccg gcggcttcgc tcgggactcg tgcagccatt ggcttctctt cgtaaggacg
     2881 ccatgcttcc ggcggctctg cgcgcgccga agcgcacttc ctaagaccga tcactgcgct
     2941 ttcgcactca cgtttcgttt gccacggcgg tgtggcgtgt atcgtgttta gctccgctgt
     3001 tgctgttgct gttgctgttg ctgttgctgt tgcctctttc gccgctgtcg cggggacgta
     3061 ttctgaggcg gagccgatgg cgcccaccgc gccgaaccaa agctcatggt ttcccacgca
     3121 gacccatccg cgacgcacgt agtgcggagt gggagctgat gagtgcgttg tcactagcgc
     3181 cgaatgtgcg agggcacgga ttccagatgc accactaccg tggctgcgcg ggggacccgc
     3241 ttaaaa
//
LOCUS       DATASH010000452         5717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4225507,
            whole genome shotgun sequence.
ACCESSION   DATASH010000452 DATASH010000000
VERSION     DATASH010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5717
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4225507"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VHV99_12515"
     CDS             complement(<1..217)
                     /locus_tag="VHV99_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010399462.1"
                     /note="cytochrome b556(FDO) component; heme containing;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HEX3636292.1"
                     /translation="MKHAEHDEHGVLKDVKGNGLIQRYTPNERTNHWITAITFVLLAL
                     SGLAMFHPAMSWLYAIFGGGQWTRILHP"
     gene            complement(204..1136)
                     /gene="fdxH"
                     /locus_tag="VHV99_12520"
     CDS             complement(204..1136)
                     /gene="fdxH"
                     /locus_tag="VHV99_12520"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0045333 - cellular respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HEX3636293.1"
                     /translation="MALQSLDIKRLSATTLPEPQVREPVTGTVAKLIDVSKCIGCKAC
                     QTACMEWNDLRDEIGTTTGTYDNPRDLSEHSWTVMRFSEYENTEGDLEWLIRKDGCMH
                     CEDPGCLKACPSPGAIVQYTNGIVDFHEENCIGCGYCITGCPFNVPRISKKDNRAYKC
                     TLCSDRVAVGQEPACVKTCPTGAIMFGTKEDMKQQAADRIVDLKERGFENAGLYDPAG
                     VGGTHVMYVLHHADKPTLYHGLPQNPKISVMVSIWKGLAKPLALAGIAFAAVAGFFHY
                     TRVGPNEVTEAEEAEAQHEVDEARRSREASDEAR"
     gene            complement(1146..4214)
                     /gene="fdnG"
                     /locus_tag="VHV99_12525"
     CDS             complement(1146..4214)
                     /gene="fdnG"
                     /locus_tag="VHV99_12525"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(3624..3626),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HEX3636294.1"
                     /translation="MTQMSRRQFLKVSATTLAGSSLALMGFSPAPALAEVRQYKLSRT
                     TETRNTCPYCLVGCGILMYGLGDNAKNAKSSIIHIEGDPDHPVNRGTLCPKGASLIDF
                     IHSESRLKYPEYRAAGSNEWQRISWEDALDRIARLMKEDRDANFVETTEDGKKVNRWL
                     TTGVLAASAGSNEVGYLTHKTVRSLGMLAFDNQARVUHGPTVAGLAPTFGRGAMTNHW
                     VDIKNADVILVIGGNAAEAHPCGFKWVTEAKAHRKARLIVVDPRFTRTASVADYYAQI
                     RTGSDIVFLGGVINYLLTNDKIQHEYVKNYTDMPFIVREDFSFTDGLYSGYDADKRKY
                     DQSSWDYERGDDGFAKVDPTLQHPRCVYQLMKQHYSRYTPEQVEKTCGTPKDKFLKVC
                     EMLASTAVPGRAGTILYALGWTHHSIGAQIIRTGAMVQLLLGNIGIVGGGMNALRGHS
                     NIQGLTDLGLMSNLLPGYMTLPMEAEQDYDAFITKRASQPLRPNQLSYWRNYRAFHVS
                     FMKSWWGDNATAENNFGYDYLPKLDKSYDMLQVFELMNQGKMNGYIAQGFNPLAAAPN
                     KAKIGAGLAKLKWLVVMDPLATETSEFWKNFGEFNDVDPSKIQTEVFRLPTTCFAEER
                     GSLVSSSRVLQWHWQGADGPGEAKSDLEIMSGLWLRIRKAYQENGGKYPDPILKMSWP
                     YSDPESPTPEELAMEFNGKALADVTDPTDKTKVLAKKGEQLASFAQLRDDGSTASGCW
                     IFCGAWTQAGNQMGRRDNSDPTGIGNTLNWAWAWPANRRILYNRASCDVSGKPFDPTR
                     KLIAWNGKAWTGADVPDFKVDEPPENGMNPFIMNPEGVARFFSRDGLVEGPFPEHYEP
                     FETPLGYNPLHPNNKAVVSNPAARVFPDDRAAFGTHENFPHTATTYRLTEHFHYWTKH
                     ARLNAIVQPQQFVEIGEDLAKEVGVVAGDRVKVSSNRGHIIAVALVTKRIKPLMIEGK
                     KVQTVGLPLHWGFKGLTKPGYLVNTLTPSVGDGNSQTPEFKSFLVKVEKA"
     gene            4464..4766
                     /locus_tag="VHV99_12530"
     CDS             4464..4766
                     /locus_tag="VHV99_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007581159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-xylosidase"
                     /protein_id="HEX3636295.1"
                     /translation="MGASAGIVSLALGSALSAISPCSFAQITQGNGSSQQENRTSSMG
                     AAPEASATAVHESQKPQTKDGASGNARGKTAGQGHKTQGAGGFDNGLYGTGAGSNK"
     gene            4939..>5717
                     /gene="selD"
                     /locus_tag="VHV99_12535"
     CDS             4939..>5717
                     /gene="selD"
                     /locus_tag="VHV99_12535"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEX3636296.1"
                     /translation="MTDTRPAANPSPRLTSLSHGGGCGCKIAPGLLADLLKRSAPLPF
                     FPDLLVGNDTADDAAVYKLNDEQAIVATTDFFMPIVDDPFDFGRIAATNALSDVYAMG
                     GKPVMALAIVGMPINVLPHDVIAAVLKGGESVCAEAGIPLAGGHSIDSVEPIYGLVAI
                     GVVDPRRVKRNADARAGDVLILGKPLGVGVLSAALKKDRLDANGYAAMIAATTKLNRP
                     GAALSMLDGVHALTDITGFGLLGHTLELARGSNLTARVRYA"
BASE COUNT         1065 a         1818 c         1660 g         1174 t
ORIGIN      
        1 agggatgcag aatccgcgtc cactgcccgc cgccgaagat cgcgtagagc cacgacatcg
       61 ccggatgaaa catggcgagg cctgataacg ccagcagcac gaacgtgatc gccgtgatcc
      121 agtgattcgt ccgttcattc ggcgtgtagc gctgaatcag accgttgccc ttcacatctt
      181 tgagcacacc gtgctcatca tgctcagcgt gcttcatcgg atgcctcccg ggaacgacgt
      241 gcttcatcga cctcgtgctg tgcttcggct tcttccgcct ccgtcacctc gttcggaccc
      301 acgcgcgtgt agtggaagaa acccgccaca gccgcgaacg cgatgccggc cagcgccagc
      361 ggtttggcca gccctttcca gatggaaacc atcacgctga tcttcggatt ctgcggcaag
      421 ccgtggtaca gggtcggctt atcggcgtga tgcagcacgt acatcacgtg cgtgccaccc
      481 acgccggcag gatcgtacaa accggcgttc tcgaaacccc gctccttcag atcgacgatc
      541 cgatccgccg cctgctgctt catgtcctcc ttggtgccga acatgatcgc gccggtgggg
      601 caggtcttca cgcatgccgg ttcctgaccg acagccacgc ggtccgaaca cagtgtgcac
      661 ttgtacgcgc ggttgtcctt cttcgaaata cgcggcacgt tgaacgggca accggtgatg
      721 caataaccac agccgatgca gttttcctcg tggaaatcca cgataccgtt cgtgtactgc
      781 acgatcgcgc ccggcgaagg acacgccttc agacagcccg gatcctcgca gtgcatgcag
      841 ccgtccttgc ggatcagcca ctcgagatcg ccttcggtat tctcgtactc agagaaacgc
      901 atgacggtcc acgaatgctc cgacagatcg cgcgggttgt cataggtgcc ggtcgtcgtg
      961 ccgatctcgt cgcgcagatc gttccattcc atgcacgccg tctgacatgc cttgcagcca
     1021 atgcacttcg acacatcgat gagcttggcg accgtgcccg tgaccggctc acgcacttgc
     1081 ggttcgggca aggtcgtggc cgagaggcgt ttgatatcca gtgattgcag tgccatctct
     1141 tttccctatg ccttttcgac cttgacgagg aacgacttga attccggtgt ctgcgaattc
     1201 ccgtcgccca cggaaggcgt cagcgtattg acgagatagc ccggcttcgt gagtcccttg
     1261 aagccccaat gcaacggcaa cccgactgtc tgaacctttt tcccttcgat catcagcggt
     1321 ttgatccgct tggtgacgag cgccacggcg atgatgtgcc cgcgattgga cgacaccttc
     1381 acacgatcgc ccgcgaccac gcccacttcc ttcgccaggt cctcgccgat ctccacgaac
     1441 tgttgcggct gcacgatcgc attcaaacgc gcgtgcttgg tccagtaatg gaaatgctca
     1501 gtcagacgat acgtggtggc cgtatgcggg aagttctcgt gcgtgccgaa tgcagcccgg
     1561 tcgtccggga acacgcgcgc cgccggattg ctgacgaccg ccttgttgtt cggatgcagc
     1621 gggttataac cgagcggcgt ttcgaacggt tcatagtgct cggggaacgg cccctccacc
     1681 agaccatcgc gcgagaagaa acgcgccacg ccttccggat tcatgatgaa cggattcatg
     1741 ccgttttcag gcggctcatc caccttgaag tcgggcacat ccgcgccggt ccacgctttg
     1801 ccgttccatg cgatcagctt gcgcgtgggg tcgaacggct tgccgctcac atcgcacgaa
     1861 gcacggttgt acagaatgcg ccggttcgcc ggccacgccc acgcccagtt cagcgtgttg
     1921 ccaatgccgg tcggatcgga gttgtcgcgg cggcccatct ggttgcccgc ctgagtccac
     1981 gcaccgcaga aaatccagca gccgcttgcc gtgctgccgt cgtcccgaag ctgcgcgaag
     2041 ctcgccagtt gctcgccctt tttggcgagg accttggttt tgtcggtggg atcggtcacg
     2101 tcggcgagcg ccttgccatt gaactccatg gcgagttcct caggcgtcgg gctttccgga
     2161 tccgaatacg gccagctcat cttgagaatc ggatcgggat acttgccgcc gttctcctga
     2221 taagccttgc ggatgcgcag ccacagcccc gacatgatct ccagatcgct cttcgcttcg
     2281 cccgggccgt ccgcgccctg ccagtgccac tgcaacacgc ggctggagct gaccagtgaa
     2341 ccgcgttctt ccgcgaaaca ggtggtcggc agacggaaga cttccgtttg aatcttcgac
     2401 ggatcgacgt cgttgaactc gccgaaattc ttccagaact cggaggtttc agtggcgagc
     2461 ggatccatga cgacgagcca cttcagcttg gccagacccg cgccgatctt cgccttgttc
     2521 ggcgccgcgg cgagcgggtt gaaaccctgc gcgatgtagc cgttcatctt gccctgattc
     2581 atcagttcga agacttgcag catgtcgtat gacttatcga gcttgggcag atagtcgtag
     2641 ccgaagttgt tctcggcggt cgcgttatcg ccccaccacg acttcatgaa gctgacgtgg
     2701 aacgcgcgat agttgcgcca gtagctgagc tggttcggcc gcagcggctg gctcgcgcgc
     2761 ttcgtgatga aggcgtcgta atcctgctcg gcttccatcg gcaaagtcat gtaacccggc
     2821 agcaggttcg acatcagccc caggtcagtc aagccctgaa tgttcgagtg gccgcgcagc
     2881 gcattcatgc cgccgcccac gatgccgatg ttgcccagca gcagttgcac cattgcgccg
     2941 gtgcggatga tctgcgcgcc gatcgagtga tgcgtccagc cgagcgcgta aaggatcgtg
     3001 ccggcgcgtc caggaacagc cgtcgatgcc agcatctcgc acaccttaag gaatttgtcc
     3061 ttcggcgtgc cacaggtttt ctcgacctgc tccggcgtat agcgcgagta atgctgtttc
     3121 atcagttgat agacgcaacg cggatgctgc agcgtcggat cgaccttcgc gaagccatcg
     3181 tcaccgcgtt cgtaatccca cgagctctgg tcgtacttgc gcttgtcggc gtcgtagccg
     3241 gaatacaaac cgtcggtaaa agagaagtcc tcacgaacga tgaacggcat gtccgtgtag
     3301 ttctttacat actcgtgctg gatcttgtcg ttggtgagca aatagttgat caccccgccc
     3361 aggaacacaa tgtccgagcc ggtccgaatc tgcgcgtaat agtccgcgac cgatgccgtg
     3421 cgcgtaaagc gcggatcgac cacgatcagg cgtgccttgc gatgcgcctt cgcttccgtg
     3481 acccacttga agccgcacgg gtgcgcctcg gccgcattgc cgccaattac cagaatcacg
     3541 tccgcgttct tgatgtcgac ccaatggttc gtcatcgctc cacggccaaa cgtcggggca
     3601 agacctgcca ccgtcgggcc atgtcagaca cgtgcctgat tatcgaatgc gagcatgcca
     3661 agactgcgga cagtcttgtg cgtcaaatac cctacttcat tgctaccggc cgatgctgcg
     3721 agcacgccgg tggtcagcca gcggttgacc ttcttgccgt cttccgtcgt ctcgacgaag
     3781 ttggcatcgc ggtcttcctt catcagtctg gcgatgcgat cgagcgcgtc ttcccaggaa
     3841 atccgctgcc attcattcga tcctgccgcg cggtactcgg gatacttcag ccggctctca
     3901 ctatgaatga agtcgatcag gctcgcgcct ttcgggcaca acgtgccgcg attcaccgga
     3961 tgatcggggt cgccttcaat gtggatgatg ctggacttcg cgttcttggc gttgtcgcca
     4021 agtccataca tcaggatgcc gcagcccacc aaacagtacg ggcaggtatt gcgggtttcg
     4081 gttgtacgcg acagtttgta ctgacggacc tcagcaagcg cgggtgccgg agagaagccc
     4141 atgagggcca aacttgatcc ggcgagcgtg gtggcggaga ccttgagaaa ctgtcgccgg
     4201 gacatctgag tcatgtaatg gcctcggctc tttatcggga tactaataac gattatagag
     4261 gcccacatga actcttgcag cgagactgca gatcttcaaa acgtcattaa atatgaagtt
     4321 ccgcagccgg tttcaacaga gtcgggcacc gttcatgctg catggttagg cggaagatca
     4381 acccttacct tggatcattc actgccatgg ctcgtcaaaa cctcgctcac tctttggcat
     4441 gcagtttcta tagcgcagcg aaattgggcg cttctgcagg catcgtttcg ctcgcgcttg
     4501 gtagcgcgct gtcggcgatc agcccgtgca gctttgctca gatcacgcaa ggcaacggtt
     4561 cctcgcagca ggaaaatcgc acctcctcca tgggcgccgc gccggaagcg tccgcaacgg
     4621 cggtccacga atcgcagaag ccgcaaacca aagacggtgc ctcgggcaac gcgcgcggca
     4681 agacggccgg ccaggggcac aagacccaag gtgccggcgg cttcgataac gggctctacg
     4741 gcactggggc gggcagcaac aaataacggg gcatgtgccg cggcttgact ggtcgcgccg
     4801 cgtgtgagta ttgcgagtgc gattgcatcg ctgccgttga ttggcctttg caacgggtaa
     4861 cgcctggtag cgactgcctg cgcggcgcgc gaacaaccgc cataatgcgg tcattcgaag
     4921 cctgagattc acgccacaat gaccgatacc cgccccgccg caaacccttc gccgcgcctg
     4981 accagcctgt cacacggcgg aggctgcggc tgcaagatcg ctcccggcct gctcgccgat
     5041 ctgctcaaac gcagcgcccc gctgccgttc tttcccgacc tgctggtcgg caacgatacc
     5101 gccgacgacg ccgccgtcta taaactcaac gacgagcagg cgatcgtcgc caccaccgac
     5161 ttcttcatgc cgatcgtcga cgacccgttc gacttcggcc gcatcgccgc aaccaatgcg
     5221 ctgtcggacg tctacgccat gggtggcaaa ccggtcatgg cgctggcgat cgtcggcatg
     5281 ccgatcaatg tgttgccgca cgacgtgatc gcggccgtgc tgaaaggcgg cgagtccgtc
     5341 tgcgccgaag ccggcattcc ccttgccggc ggccattcga tcgattcggt ggaacccatt
     5401 tacggcctcg ttgcgatcgg cgtggtcgat ccgcggcgcg tcaaacgcaa tgcagatgcg
     5461 cgggcgggcg acgtgctgat cctcggcaag ccgctcggcg tgggcgtgct gtcggcggcg
     5521 ttgaaaaaag accggctcga tgcaaacggc tatgccgcga tgatcgcggc caccaccaag
     5581 ctcaaccggc cgggcgcggc gctctccatg ctcgacggcg tgcatgcgct cacggacatc
     5641 accggcttcg gcctgctcgg tcacacgctg gagctggcgc gcggctcgaa cctcaccgct
     5701 cgcgtgcgtt atgccga
//
LOCUS       DATASH010000453         7502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3037874,
            whole genome shotgun sequence.
ACCESSION   DATASH010000453 DATASH010000000
VERSION     DATASH010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7502
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3037874"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2463)
                     /locus_tag="VHV99_12540"
     CDS             complement(<1..2463)
                     /locus_tag="VHV99_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX3636297.1"
                     /translation="MNLSRPFISRPVATTLLAIGIALAGIFAFTKLPVAPLPQVDFPT
                     ISVQATLPGASPDTVATSVASPLERHLGSIADVTEMTSMSSVGSTRITMQFGLNRDID
                     GAARDVQAAINAARADLPASLRSNPTYHKVNPADAPILILALTSKTLTAGQLYDSAAT
                     VLQQSLSQVDGVGEVDVSGSANPAVRVELEPNALSHYGIGLEDVRAALASANANSPKG
                     SIEFGPNRVQIYTNDQASKAAQYKDLIIAYRNGSAVKLSDVAEVVDSVEDLRNLGLFN
                     GKRSVLVILYRQPGANIIDTIDRVMAMLPQLHASLPADVDIAPAADRSTTIRASLKDT
                     ERTLIIAVALVTMVVFLFLRNWRATLIPTVAVPISIIGTFAAMYLLGFSIDNLSLMAL
                     TIATGFVVDDAIVVLENISRHIENGVPRMQAAILGAREVGFTVLSISISLVAVFLPIL
                     LMGGIVGRLFREFALTLSLAIGVSLIVSLTLTPMMCSRFLREAHEKKEEGRFGLWLER
                     GFTSMQRGYERTLGWALLHPRLIVTILLLTIGLNIWLYIIVPKGFFPQQDTGRLVGGI
                     QADQSTSFQAMKGKFAEMMEIVGKNPAVESVAGFTGGRQTNSGFMFVSLKPKSERKLS
                     ADQVIQQLRAPLGDVAGARTFLQAVQDIRVGGRQSNAQYQFTLLADSTPDLYLWGPRL
                     TEALQARPELADVNSDQQQGGLEAMVTIDRASAARLGITPSQIDNTLYDAFGQRQVST
                     IYNPLNQYHVVMEVAQKYWQSPDMLKQIYISTSGGSASGAQTTNAPAGTVTSKETEKT
                     TATTSAATGGAAGTTASSAASIA"
     gene            complement(2467..5601)
                     /locus_tag="VHV99_12545"
     CDS             complement(2467..5601)
                     /locus_tag="VHV99_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MdtB/MuxB family multidrug efflux RND
                     transporter permease subunit"
                     /protein_id="HEX3636298.1"
                     /translation="MNPSRAFILRPVGTALLMAAIMLVGLVALRFLPLSALPEVDYPT
                     IQVQTFYPGASPDVMTSSVTAPLERQFGQMPSLNQMSSQSSAGSSIITLQFSLDLPLD
                     IAEQEVQAAINAAGNLLPSDLPAPPIYAKVNPADAPVITLAITSKTLPLTQVQDLADT
                     RLAQKISQVAGVGLVSLSGGQRPAVRIQANPRALAAYGLNLDDLRTTISNLNVNTPKG
                     NFDGPTRNYTINANDQLTDAEAYKSAVVAYKNGRPVMLTDVATIVQGAENTKLGAWVD
                     NTPAIILNVQRQPGANVIQVVDSIKALLPQLQQSLPAALDVQIVTDRTTTIRASVRDV
                     QFELLMSVVLVVLVMYLFLANVYATIIPSLSVPLSLIGTLAVMYLCGFSLDNLSLMAL
                     TIATGFVVDDAIVMIENIARYVEDGESPLEAALKGSKQIGFTIISLTVSLIAVLIPLL
                     FMGDVVGRLFHEFAITLAVTIVISAIVSLTLVPMMCAKLLRHSPPHESHRFEAKAHNA
                     IDWVIARYAVALTWVLNRQRSTLFVALLTLVLTGVLYVYIPKGFFPVQDTGVIQAITQ
                     APQSVSYASMAERQQELAAQILKNPDVESLTSFIGVDGSNITLNSGRMLINLKPRDDR
                     SNTASEVIRQLQQDVAHIAGASLYMQPVQDLTIDATVSPTQYQFMLTDPNISEFTTWV
                     PKLVDRLKQSPELADVATDLQDNGQSVYIEIDRATASRYGITPATVDSALYDAFGQRI
                     ISTIFTQSNQYRVILEAQPQMQHYTESLNSIYLPSSTSSGGQVPLSSIARFVERPAPL
                     LVSHLSQFPATTVSFNLAPGSSLGAAVKAIDQAEKDIGLPASFQTRFQGAALAFQASL
                     SNELFLILAAIITMYIVLGVLYESFIHPITILSTLPSAGVGALLALLITGHDLDIIGI
                     IGIVLLIGIVKKNAIMMIDFALYAEREEGKSPREAIYQACLLRFRPILMTTLAALLGA
                     LPLMLGTGAGSELRRPLGIAIAGGLIVSQLLTLFTTPVIYLGFDALARRARARFHRGD
                     ASKGAPPAATDAGN"
     gene            complement(5620..7047)
                     /locus_tag="VHV99_12550"
     CDS             complement(5620..7047)
                     /locus_tag="VHV99_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MdtA/MuxA family multidrug efflux RND
                     transporter periplasmic adaptor subunit"
                     /protein_id="HEX3636299.1"
                     /translation="MDEQQKHSETSRPAPPAAQPGDSQPAASGKPAGGSGYPPLEPKS
                     HRGRNIALLVAALVILGIVLWRWHPWGGPGGGASGAAGAGGASGGHAGRGGRGGGGFA
                     NMAQPVHVASAVQGEMPVVLTALGTVTPLANVTVLPQLSGMLQDVYFKEGQIVRKGDL
                     LAQIDPRPYQISLENAQGTLARDQALLQTARLDLKRYQTLLSQDSIASQQVDTQASLV
                     RQYEGTVKSDQANIDTFKLDLIYARITAPVSGRVGLRQVDPGNYVTPSLTNGLVVITQ
                     LQPISVIFTTSEDNLQQILQQTQTGAKLSVTAYDRSNTTSLEGGSLETLDNQIDTTTG
                     TLKLRATFPNDKNVLFPNQFVNTRLLVDTIKDAVIVPTPAVLNGSMGQFVYVVKPDNT
                     VTVRPVKIGPVDGERTSIKSGLQVGERVVIDGSDRLKEGAKITIPADKPRGASGARGA
                     SGAAAASGASGAHGHHRRHAAQASE"
BASE COUNT         1322 a         2327 c         2494 g         1359 t
ORIGIN      
        1 ggcgatgctc gccgcgctcg acgcggtggt gcccgcagca ccgccggtgg ccgcgctggt
       61 ggtggcggtg gtcttctcgg tttccttcga cgtgaccgtg cccgccggcg cattggtggt
      121 ctgcgcgccg ctcgcgctgc cgcccgacgt gctgatgtaa atctgcttca gcatgtccgg
      181 actctgccag tacttctgcg cgacttccat cacgacgtgg tactggttca gcgggttgta
      241 gatcgtcgag acctggcgct ggccgaacgc gtcgtacaag gtgttgtcga tctgcgacgg
      301 cgtgatgccg aggcgcgccg cgctcgcgcg gtcgatcgtc accatcgcct cgaggccgcc
      361 ctgctgctgg tcggaattca cgtcggcgag ttcggggcgc gcttgcagcg cttcggtgag
      421 cctcggtccc cagagataca agtcaggcgt ggaatcggcc agcagcgtga actgatactg
      481 ggcgttcgat tgccggccac ccacgcgaat gtcctgcacc gcttgcagga aagtccgcgc
      541 accggccaca tcgccgagcg gggcgcgcag ttgctggatc acctggtcgg cggagagctt
      601 gcgctcgctc ttcggtttca gcgacacgaa catgaagccc gagttggtct gccggccgcc
      661 ggtgaaacca gccacgctct ctaccgcggg attcttgccg actatctcca tcatctccgc
      721 gaacttgccc ttcatcgcct ggaacgacgt gctctggtcg gcctgaatgc cgccgaccag
      781 ccggcccgtg tcctgttggg gaaagaagcc cttcggcaca atgatgtaca gccagatgtt
      841 cagtccgatc gtcaacagca gaatcgtcac gatcaggcgc ggatgcagca gcgcccagcc
      901 gagggtgcgc tcataaccgc gctgcatcga cgtgaagccg cgttcgagcc agaggccgaa
      961 acgtccttct tcctttttct cgtgcgcctc gcgcaggaaa cgcgaacaca tcatcggcgt
     1021 caaggtcagc gagacgatca gcgacacgcc gatcgccagc gacagcgtca gcgcgaactc
     1081 gcggaacagc cgcccgacaa tgccgcccat cagcagaatg ggcaggaaca cggccaccag
     1141 cgaaatactg atggacagca ccgtgaagcc gacttcgcgc gcgccgagga tcgcggcctg
     1201 catgcgcggc acgccgtttt ctatatgccg tgagatgttc tccagcacca cgatcgcatc
     1261 gtcgaccacg aagccggtcg cgatcgtcaa cgccatcagc gacaggttgt cgatcgagaa
     1321 gccgagcaga tacatcgcgg cgaacgtgcc gatgatcgag atcggcacgg cgacggtcgg
     1381 aatcaacgtt gcgcgccagt tgcgcaggaa caggaacacc accatcgtga ccagcgcgac
     1441 ggcaatgatc aacgtgcgct cggtgtcttt cagcgacgcg cggatcgtcg tggagcggtc
     1501 ggcggccggc gcgatgtcga cgtcggccgg cagcgacgcg tgcaattgcg gcaacatcgc
     1561 catcacgcgg tcgatcgtgt cgatgatgtt cgcgccgggc tggcggtaca gaatcaccag
     1621 caccgaacgc ttgccgttga agaggccgag gttgcgcaga tcctcgaccg aatcgaccac
     1681 ttccgccacg tcggacagtt tcaccgccga gccattccgg tacgcgatga tcagatcttt
     1741 atactgcgcg gccttgctcg cctggtcgtt ggtatagatc tgcacgcggt tggggccgaa
     1801 ttcgatcgag cctttcgggc tgttcgcgtt ggccgaggcg agcgccgcgc gcacgtcttc
     1861 aaggccgatg ccgtagtggg acagggcgtt cggttccagt tcgacacgca ccgccggatt
     1921 ggccgagccg ctcacgtcca cttcgccgac gccatccact tgcgagagcg actgctgcaa
     1981 cacggtggcc gccgagtcgt acaactggcc ggcagtcagc gtcttcgacg tgagcgcgag
     2041 gatcaggatc ggcgcgtcgg ccggattcac cttgtggtat gtcggattgc tgcgcaagct
     2101 cgccggcaga tcggcacggg cggcgttgat ggccgcttgc acgtcgcgcg cggcgccgtc
     2161 gatgtcgcgg ttcaggccga actgcatcgt gatgcgcgtt gagccgaccg agctcatcga
     2221 ggtcatttcg gtgacgtcgg cgatcgagcc gagatgccgt tcgagcggac tggctacgct
     2281 cgtcgccacc gtgtccggac tcgcgcccgg caatgtggcc tgcacggaaa tggtcgggaa
     2341 gtcgacctgc ggcagcggcg cgaccggcag tttggtgaac gcgaaaatac cggccagcgc
     2401 gatgccgatc gccaacagcg tggtggcgac cgggcgggag ataaacggac gcgacaggtt
     2461 catcgcttag ttccctgcat cggtggcggc cggcggcgcg cctttcgacg catcgccgcg
     2521 atggaaccgc gcgcgcgcgc ggcgggccag cgcatcgaag ccgaggtaaa tcaccggcgt
     2581 ggtgaacagc gtcagcagct ggctgacgat cagcccgccg gcaatcgcga taccgagcgg
     2641 gcggcgcagt tccgaaccgg cgccggtgcc gagcatcagc ggcagggcgc cgagcagcgc
     2701 ggcaagcgtg gtcatcagaa tcggccggaa gcgcagcaga cacgcctgat aaatcgcctc
     2761 gcgcggcgac ttgccttcct cacgctcggc gtagagcgcg aagtcgatca tcatgatggc
     2821 gttcttcttc acgataccga tcagcagcac gataccgata atgccgatga tgtccagatc
     2881 gtgcccggtg atcaggagcg ccagcaatgc gccgacgccg gccgacggca gcgtcgacag
     2941 aatcgtgatc gggtgaatga agctttcgta cagcacgccg agcacgatat acatcgtgat
     3001 gatcgccgcc aggatcagga acagttcgtt cgacaacgac gcctggaacg ccagcgccgc
     3061 gccctgaaag cgtgtctgga acgaggccgg cagcccgata tccttctccg cctgatcgat
     3121 cgctttcacc gccgcgccaa gcgatgagcc gggtgcgagg ttgaacgaga cggtggtggc
     3181 cgggaactgg ctcaaatgcg agaccagcag cggcgcgggc ctctcgacaa agcgcgcgat
     3241 cgacgacagc ggcacctgcc cgcctgacga cgtggaggag ggcagataga tcgagttcag
     3301 cgactcggtg taatgctgca tctgcggctg cgcttcgaga atcacgcggt actggttcga
     3361 ctgcgtgaag atggtcgaaa tgatgcgctg gccgaacgcg tcgtagagcg cgctgtcgac
     3421 ggtagccggt gtgatgccgt agcgcgacgc cgtggcgcgg tcgatctcga tgtagaccga
     3481 ctggccgtta tcctgcaggt cggtcgccac gtcggccagt tcaggcgact gcttcaaccg
     3541 gtcgaccagc ttcggcaccc acgtggtgaa ttcgctgatg ttcgggtcgg tcagcatgaa
     3601 ctggtactgc gtcgggctga ctgtggcatc gatggtcaga tcctgcaccg gctgcatgta
     3661 cagcgacgcg cccgcaatgt gcgccacgtc ctgctgcaac tggcggatca cctcgctcgc
     3721 ggtgttgctg cggtcgtcgc gcggcttgag gttgatcagc atgcggccgc tattcaacgt
     3781 aatgttgctg ccgtccactc cgatgaacga ggtgaggctt tccacgtccg gattcttcag
     3841 gatctgcgcg gcgagttcct gctgacgctc ggccatcgac gcatacgaca ccgactgcgg
     3901 cgcctgcgtg atcgcctgga tcacgccggt gtcctgcacc gggaagaagc ccttcgggat
     3961 gtacacgtac agcacgccgg tcagcaccag tgtgagcagc gcgacgaaca gcgtggagcg
     4021 ttgccggttc agcacccagg tgagcgcgac cgcatagcgt gcgatgaccc agtcgatcgc
     4081 gttgtgagct ttggcttcga aacggtggct ttcatgtggc ggcgagtgac gcaggagctt
     4141 cgcgcacatc atcggcacga gcgtgagcga gacgatggcc gagatcacga tcgtcacggc
     4201 cagcgtgatc gcgaattcgt ggaacaggcg cccgaccacg tcgcccatga agagcagcgg
     4261 gatcagcacg gcgatcagcg aaaccgtcag cgaaatgatc gtgaagccaa tctgtttcga
     4321 gcctttcagc gcggcttcca atggcgattc gccgtcttcg acatagcgcg cgatgttctc
     4381 gatcatcacg atggcatcgt ccaccacgaa gccggtggcg atggtcagcg ccatcagcga
     4441 caggttatcc agcgagaacc cgcacaggta catcacggca agcgtgccga tcagcgaaag
     4501 cggcaccgac aggctcggga tgatggtggc gtagacgttg gcgaggaaca ggtacatcac
     4561 cagcaccacc agtacgaccg acatcagcag ttcgaactgc acgtcgcgca cggaagcgcg
     4621 gatcgtggtg gtgcggtcgg tgacgatctg cacgtcgagc gcggcgggca gcgactgctg
     4681 cagttgcggc agcagtgcct tgatgctgtc gaccacctgg atcacgttgg cgcccggctg
     4741 gcgctgcacg ttcaggatga tggccggggt gttgtcgacc cacgcgccga gtttggtgtt
     4801 ctccgcgcct tgcacgatgg tggcgacgtc cgtcagcatg accgggcggc cgttcttgta
     4861 cgcgaccacg gcgctcttgt aagcctcggc gtcggtcagc tggtcgttcg cgttgatcgt
     4921 gtagttgcgc gtcgggccgt cgaagttacc cttcggcgta ttgacgttca ggttcgaaat
     4981 cgtggtgcgc agatcgtcca gattcagccc gtacgcggcg agcgcgcgcg gattcgcctg
     5041 aatccgcacg gccgggcgtt gaccgcctga caggctcacg agacccacac ccgccacctg
     5101 cgagatcttc tgcgcgagac gcgtgtcggc cagatcctgc acctgcgtga gcggcagcgt
     5161 tttcgaggtg atcgcgagcg tgatgaccgg tgcgtcggcg ggattgacct tggcgtaaat
     5221 cggcggcgcg ggcaggtcgg acggcagcag gttgcccgcg gcgttgatgg cggcctggac
     5281 ttcctgttcg gcgatatcga gcggcagatc gaggctgaac tgcagcgtga tgatcgacga
     5341 gccggccgag ctttgcgacg acatctggtt caacgacggc atctgcccga actggcgttc
     5401 gagcggcgcg gtgacggaag acgtcatcac gtccgggctc gcgcccggat agaaggtctg
     5461 cacctggatg gtcggatagt cgacttcggg cagcgcggaa agcggcaaaa agcgcagcgc
     5521 gacgagaccg accagcatga tcgccgccat cagcagcgcg gtgccgacgg gacgcagaat
     5581 aaaggcgcgg gatggattca tgcggtagtg gccgtgcctt tattccgagg cttgcgccgc
     5641 gtgtctgcga tgatgtccat gtgcgccgga agcgcccgac gccgccgccg cgccgcttgc
     5701 tccacgcgcg cccgaggcgc cgcgtggctt gtcggccgga atcgtgattt tcgcgccttc
     5761 cttcaggcgg tccgacccgt cgatcacgac gcgttcgccg acctgcaggc cggatttgat
     5821 actggtgcgc tcgccgtcca ccggaccgat cttgaccgga cgcacggtca ccgtgttgtc
     5881 cggcttcacg acatacacga actgacccat cgagccgttc agcacggccg gcgtcggcac
     5941 gatcaccgcg tccttgatcg tgtccacgag aaggcgtgta ttcacgaact gattcgggaa
     6001 cagcacgttc ttgtcgttcg ggaaagtggc gcgcagcttg agcgtgccgg tggtggtatc
     6061 gatctggtta tccagtgttt cgagcgagcc gccttcgagc gaggtggtgt tgctgcggtc
     6121 ataggcggtg accgacagct tcgcgcccgt ttgggtctgc tggaggatct gctgcaggtt
     6181 gtcttcggag gtcgtgaaga tcacgctgat cggctgcaac tgcgtaatca cgacgaggcc
     6241 gttggtcagg ctcggcgtca cgtagttgcc cggatcgacc tggcgcaaac cgacgcgccc
     6301 cgataccggc gcggtgatgc gtgcatagat gaggtcgagc ttgaaggtat cgatgttcgc
     6361 ctggtcggat ttcaccgtac cttcatattg ccgcacgagc gaggcctggg tatcgacctg
     6421 ctggctcgcg atcgagtctt gcgagagcag cgtctgataa cgcttcaggt cgaggcgggc
     6481 ggtttgcagc agcgcctggt cgcgcgcgag ggtgccttgc gcgttttcca gcgagatctg
     6541 ataggggcgc gggtcgatct gggcgagcag gtcgcccttc ctgacaatct ggccttcctt
     6601 gaaatagacg tcctgcagca tgccgctcaa ctgcggcaac acggtgacgt tggcgagcgg
     6661 tgtgaccgtg ccgagcgcgg tcagcaccac cggcatttca ccctgcacgg cgctcgccac
     6721 atgaaccggt tgcgccatgt tggcgaagcc gccaccaccg cggccgccac gccccgcgtg
     6781 gcccccgctt gcaccgcctg cgccggcggc tccgctcgcg ccgccgccgg ggccgcccca
     6841 cggatgccag cgccacagca cgatcccgag gatgacgagc gccgccacga gcagcgcgat
     6901 gttgcggccg cgatggcttt tcggttcgag cggcgggtat cccgaaccgc cagcgggctt
     6961 gccgctagcg gctggttgcg aatcaccggg ttgggcggcc ggcggggcag ggcgtgaggt
     7021 ttccgaatgc ttttgttgtt cgtccatcga tcggtcaggt ggctggcttt gatgtgagcc
     7081 gctcgtgcga ttctgccgct tcgacaattc agcggaccgg cacgcaggcg gcaggacagg
     7141 cggtttcgac agcgggcgcg ccgcgcatgt tcaacgcgat gctacccgag gcgcgggaca
     7201 aagatgttaa agcaatgcgg catcatgcag gtcggccgga ccgttatcaa aggatgagga
     7261 cgccaagccc ggcgaacgcc ccgcgacagg aaggcaagat cctacgtcgc aggctctgta
     7321 acagtccagc aatgtatttt tctcttgatt acgaaggtta cagaatcaca acgcgcctgg
     7381 aatgccgttt ccgcccgccg ccgggtgtct gcgcctttca ggtgtccagc cggcgcccag
     7441 gggttccgcc gatctcgagc acgatgttac tgatacattc gagcagggcc ggcgcggcgc
     7501 gc
//
LOCUS       DATASH010000454         5167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4817840,
            whole genome shotgun sequence.
ACCESSION   DATASH010000454 DATASH010000000
VERSION     DATASH010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4817840"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /gene="tatB"
                     /locus_tag="VHV99_12555"
     CDS             complement(<1..262)
                     /gene="tatB"
                     /locus_tag="VHV99_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HEX3636300.1"
                     /translation="MLDLGLTKMALIGVVALVVLGPERLPRVARTAGALFGRAQRYIN
                     DVKAEVTREIELDELRRMKSEFEAAATNVETSVQDNLRKHETE"
     gene            complement(302..541)
                     /gene="tatA"
                     /locus_tag="VHV99_12560"
     CDS             complement(302..541)
                     /gene="tatA"
                     /locus_tag="VHV99_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HEX3636301.1"
                     /translation="MGSLSIWHWLIVLLIVALVFGTKKLRNIGGDLGGAVKGFKEGMK
                     EAETPAGEAQQRELPRNGAVDVEAKEKTPRSGDYR"
     gene            complement(783..1148)
                     /locus_tag="VHV99_12565"
     CDS             complement(783..1148)
                     /locus_tag="VHV99_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEX3636302.1"
                     /translation="MSHDSNCLFCKIAAGEIPSTKVHEDEEFFAFRDIRPAAETHVLV
                     IPRKHIATLSNCTESDAPLLGRMLVLVARLADQLGVAYTGGETGFRTVINTGPGGGQE
                     VYHLHAHILAGPRPWLRMG"
     gene            complement(1230..1625)
                     /locus_tag="VHV99_12570"
     CDS             complement(1230..1625)
                     /locus_tag="VHV99_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636303.1"
                     /translation="MEQSQERYPPPVYRNALEPERERSLRTLTHVLYALYAVHWLTGG
                     LTILIAIIINYVKRADVVGTPYEAHFEWQIRSFWMALLGYAIGGVLLFVVIGIPILWA
                     VSIWMLYRIIKGWLYLYDNKPLANPRGWF"
     gene            complement(1720..2112)
                     /locus_tag="VHV99_12575"
     CDS             complement(1720..2112)
                     /locus_tag="VHV99_12575"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEX3636304.1"
                     /translation="MTQFTQSTESASSSSDTLLRLAAIIDSRKGGDPDVSYVSRLFHK
                     GDDAILKKIGEEATEVVLAAKDARHGGAPKALVGEVADLWFHCLVMLSHFDLSPADVL
                     AELERREGLSGIEEKALRKSREREQNGD"
     gene            complement(2109..2519)
                     /gene="hisI"
                     /locus_tag="VHV99_12580"
     CDS             complement(2109..2519)
                     /gene="hisI"
                     /locus_tag="VHV99_12580"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEX3636305.1"
                     /translation="MVNPSAVDWLDKVRWDANGLVPVIAQEASTNDVLMFAWMNREAL
                     AKTVELGRAVYFSRSRQRLWFKGEESGHVQHVHEVRLDCDEDVVLLKVEQVSGIACHT
                     GRHSCFFQKFEGTVEDGGWVAVDPVLKDPEHIYK"
     gene            complement(2521..3294)
                     /gene="hisF"
                     /locus_tag="VHV99_12585"
     CDS             complement(2521..3294)
                     /gene="hisF"
                     /locus_tag="VHV99_12585"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012362933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEX3636306.1"
                     /translation="MALAKRIIPCLDVTAGRVVKGVNFVELRDAGDPVEIARRYDDQG
                     ADELTFLDITATSDQRDLILPIIEAVASQVFIPLTVGGGVRAVEDVRRLLNAGADKIS
                     MNSSAVANPQLVRDATDKYGSQCIVVAIDAKRVSADGEAPRWEVFTHGGRKATGLEAV
                     EWARKMAELGAGEILLTSMDRDGTKSGFDLALTRAVSDAVSIPVIASGGVGSLQHLAD
                     GIKSGHADAVLAASIFHYGEHTVGEAKRFMADQGISVRL"
     gene            complement(3559..4311)
                     /gene="hisA"
                     /locus_tag="VHV99_12590"
     CDS             complement(3559..4311)
                     /gene="hisA"
                     /locus_tag="VHV99_12590"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007731840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEX3636307.1"
                     /translation="MLLIPAIDLKDGHCVRLKQGDMDQATIFSEEPAAMARHWVDRGA
                     RRLHLVDLNGAFAGKPKNEDAIRAIIEEVGGEIPVQLGGGIRDLNTIERYLDDGLSYV
                     IIGTAAVKNPGFLQDACTAFGGHIIVGLDAKDGKVATDGWSKLTGHEVADLARKFEDY
                     GCESIIYTDIGRDGMLQGINIEATVRLARAVKIPVIASGGLSNLADIESLCEVEDEGI
                     EGVICGRAIYSGDLDFAAAQTLADRLRESDDA"
     gene            complement(4526..>5167)
                     /gene="hisH"
                     /locus_tag="VHV99_12595"
     CDS             complement(4526..>5167)
                     /gene="hisH"
                     /locus_tag="VHV99_12595"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEX3636308.1"
                     /translation="MKTSIAIVDYGMGNLRSVAQALRKAAPEAEVAIVDQPEAIRSAD
                     RVVLPGQGAMPDCMRSLAESGLQEAVIEASRSKPLMGVCVGEQMLFDWSAEGGTPGLG
                     LLPGKVLRFDLEGQVQDDGSRFKVPQMGWNRVRQAQPHPLWDGVADNAFFYFVHSYYV
                     VPDNAAHTSGETVYGVPFTSAVARDNIFATQFHPEKSAEAGLRVYRNFVHWNP"
BASE COUNT          949 a         1705 c         1534 g          979 t
ORIGIN      
        1 gttcggtctc atgcttgcgc agattgtcct gaacggaggt ctcgacgttg gtcgcggccg
       61 cttcgaactc gcttttcata cgacgcagct cgtcgagttc gatttcgcgc gtgacttcgg
      121 ccttcacgtc gttgatatac cgctgcgcgc ggccgaacaa cgcgccggcc gtgcgggcaa
      181 cgcgtggcag gcgctcaggg ccgagtacga ccagcgcgac gacgccgatc agcgccatct
      241 tggttagacc gaggtccagc atgaagtgaa gtgtccgtca gtaacgcgga tgggccgcgg
      301 cttagcggta atcgccggaa cgcggggtct tttccttcgc ttccacatcc acggcgccgt
      361 tgcgcggcag ttcgcgctgc tgcgcttcgc ctgcgggcgt ttcggcttcc ttcatgcctt
      421 ccttgaagcc cttcaccgcg ccacccagat cgccgccgat attgcgcagc tttttcgtgc
      481 cgaacacgag cgccacgatc aacaacacga tcagccaatg ccaaatgctc aacgaaccca
      541 tgactactct ccttgacccc gacaccgcaa gtgtgcggca aaacttgtac gccctgcggc
      601 gtgaatcgaa gcatgtacgc ttcatctaaa cgttagcgcg ggctgttccg actccaccca
      661 cgatacgctg tgtcattgtg atcgccgatc acgtcggcaa cattacaatg cagtaaaaaa
      721 taaccgccgc gctattgtcg acgaccaaaa ctcgccgcgt gcttccggcg cacgattttc
      781 tgtcagccca tccgcaacca cgggcgcggc cccgcgagga tgtgcgcgtg caagtgatac
      841 acctcttgcc cgccgcccgg cccggtattg attaccgtgc gaaaaccggt ttcgccaccg
      901 gtgtacgcca cgccaagctg gtcggccaga cgcgccacca agacaagcat tctaccaagc
      961 agcggcgcat cactttcggt gcaattcgac agcgtcgcga tgtgtttgcg aggaatcacc
     1021 agcacatgcg tttcagccgc tggacgaatg tcgcggaagg caaaaaactc ttcgtcttcg
     1081 tggactttgg tcgacggaat ctcaccggcg gcaatcttgc agaaaaggca gttcgaatcg
     1141 tggctcatcg tattcctgac gcagtgtggc tagcgtgcga aacgcgttgg ggcaagtgca
     1201 tgtcgagccc ggtcacaacc ggactcaact cagaaccagc cacgcgggtt cgcaagcggc
     1261 ttgttatcgt acagatatag ccagcctttg ataatacggt acaacatcca gatactgacg
     1321 gcccacagga tcggaatacc gatcaccacg aacagcagca cgccgccgat cgcataaccc
     1381 agcaaggcca tccagaacga gcggatctgc cactcgaaat gcgcctcgta aggcgtgccc
     1441 accacgtcgg cccgcttcac gtagttgatg atgatcgcga tcagaatcgt cagaccaccc
     1501 gtcagccaat ggaccgcata cagcgcgtac agcacatgtg tgagcgtgcg cagactgcgt
     1561 tcgcgctccg gctcgagcgc gttgcggtag accggcggcg gatacctctc ctgcgactgt
     1621 tccatgcttg cgtcctcccg gggatgtgat cccatgcgtt cagtgcaatg tgggtgccga
     1681 cgttcatcac ttcaagacag ggcggacaaa cccggacaat cagtcgccgt tctgctcgcg
     1741 ctcgcggctc ttgcgcagcg ccttttcctc gatgcccgac aggccttcac ggcgctcgag
     1801 ttcggcgagc acgtcggccg ggctcaggtc gaaatgcgac agcatcacga ggcagtgaaa
     1861 ccacaggtcc gccacttcgc cgaccagtgc tttcggcgcg ccgccgtggc gcgcgtcttt
     1921 ggcggccagc acgacttcgg tggcttcttc gccgatcttc ttgagaatcg cgtcatcgcc
     1981 cttgtgaaac aggcgcgaga cgtacgagac gtccggatcg ccgcccttgc ggctgtcgat
     2041 gatggccgcg agacgcagca gcgtgtcgct gctggacgaa gcggattcgg tggattgcgt
     2101 gaattgcgtc atttgtagat gtgttcgggg tctttcagca cgggatcgac ggcgacccag
     2161 ccgccgtctt caaccgtgcc ttcgaatttc tggaaaaagc acgagtggcg gccggtgtga
     2221 caggcgatgc ccgacacctg ctcgaccttc agcagcacga cgtcttcgtc gcaatcgagc
     2281 cgcacttcat gcacatgctg cacgtggccg gactcttcgc ccttgaacca aaggcgctgg
     2341 cgcgaacgcg agaaatacac cgcgcggccc aactccaccg tcttcgccaa cgcttcgcgg
     2401 ttcatccacg cgaacatcaa cacgtcgttg gtcgacgctt cctgcgcgat cacgggcacg
     2461 aggccgttcg cgtcccaccg gaccttgtcg agccaatcta ccgctgaagg attcaccacg
     2521 tcacaacctc accgaaatgc cttgatcggc catgaagcgc ttggcctcgc ccacggtgtg
     2581 ttcgccgtag tggaagatgc tggcggccag caccgcgtcc gcgtgaccgc tcttgatgcc
     2641 gtcagccaga tgctgcagcg aaccgacgcc gcccgaggcg atcaccggaa tcgacaccgc
     2701 atcggacacc gcgcgcgtga gcgcgagatc gaagccgctc ttggtgccgt cgcggtccat
     2761 gctggtcagc aggatttcgc ccgcgccgag ttcggccatc ttgcgcgccc attcgaccgc
     2821 ttcgaggccg gtggccttgc gcccgccatg cgtgaagact tcccagcgcg gcgcttcgcc
     2881 gtccgcggag acgcgcttcg cgtcaattgc gacgacgatg cattgcgagc cgtatttgtc
     2941 cgtcgcgtcg cgcacgagtt gcggattcgc gaccgccgac gagttcatgc tgatcttgtc
     3001 cgcgccggcg ttgagcaagc gccgcacgtc ttcgacagcg cgcacgccgc cgccgaccgt
     3061 cagcggaatg aacacctgcg aggcgaccgc ttcgatgatc ggcaggatca gatcgcgttg
     3121 atcggaggtc gcggtgatgt cgaggaaagt gagttcgtcg gcgccctggt cgtcgtaacg
     3181 gcgcgcgatt tcgaccgggt cgcccgcgtc gcgcagttcg acgaagttga cgcccttgac
     3241 cacgcgtcca gccgtgacgt cgagacaggg gatgatgcgt ttagctagag ccatgatctt
     3301 gcaattctgc caataccgct gatgaggccg ctcgcctgac acttcacgag cggcacagtg
     3361 tcggcgcggc gagccgcgtc ggaccgaata tgtgccaaga cacatgcgtg cggcttgacg
     3421 cgcttgcgat gcgccgttgc gtccgcttcc cgtccgcttt tgcgcctgct tgagcccgcc
     3481 tgatgcccgc ccgatgcccg cgttacgccc ggcaatgcgc cgatgccgcg gcgaatgcga
     3541 cgcagcaggc gccgaacctc aagcgtcgtc cgattcgcgc agccggtccg cgagcgtttg
     3601 agccgccgcg aagtccagat cgcccgagta gatcgcccgg ccgcagatca cgccttcgat
     3661 gccctcgtcc tcgacttcgc acagcgactc gatgtccgcg agattcgaca atccgccgct
     3721 cgcgatcacc ggaattttca ccgcgcgggc aaggcgcacc gtcgcttcga tgttgatacc
     3781 ctgcagcatg ccgtcgcggc caatgtcggt gtagatgatc gattcgcagc cgtaatcctc
     3841 gaacttgcgc gccagatcgg ccacttcgtg gccggtcagc ttgctccagc cgtcggtggc
     3901 gaccttgcca tctttcgcat ccagaccgac gatgatatgg ccgccgaagg ccgtgcacgc
     3961 atcctgcagg aaacccggat tcttcaccgc agccgtgccg atgatgacgt acgacaaacc
     4021 gtcgtccaga tagcgctcga tcgtgttcag gtcacggata ccgccgccca gctggacggg
     4081 aatctcgccc cctacttcct cgatgatcgc gcggatcgcg tcttcgtttt tcggcttgcc
     4141 ggcgaacgcg ccattcaggt cgacgaggtg cagacggcgg gcaccgcggt cgacccaatg
     4201 ccgggccatg gccgccggtt cctcggaaaa tatcgtcgcc tggtccatat cgccctgttt
     4261 gaggcgtaca cagtgaccat ctttcaggtc gatggcggga atcagcagca tagcaatcgg
     4321 gtgttgtctg ggaggtggtt gaaggtcggc ggcagcggct gacaggccag cccgccacaa
     4381 cgccgtttcg ctagtttagt acaactcttt cgacgccctt gcggagcgcc atattgaagc
     4441 gaaattgccg cactgccgtg gtggcgcacg ggcggcgctc cgctcgtggc ggaacggcgc
     4501 tcgacagcag gatgaagaaa agcgctcacg ggttccagtg cacgaagttg cgatacacgc
     4561 gcagccccgc ctcagcgctc ttttccgggt ggaattgggt cgcgaagatg ttatcccgcg
     4621 ccaccgccga ggtaaagggc acaccgtaca ccgtttcgcc cgaggtatga gcggcgttgt
     4681 ccggcacgac gtaatagcta tgcacgaagt agaagaacgc gttgtccgca acgccgtccc
     4741 acaacggatg cggctgcgcc tggcgcacgc ggttccagcc catttgcggg accttgaagc
     4801 gcgagccgtc gtcctgcacc tggccttcca ggtcgaagcg cagcaccttg ccgggcaaca
     4861 gacccaggcc gggcgtaccg ccctcggcgc tccagtcgaa cagcatctgc tcgccgacgc
     4921 acacgcccat cagcggcttg ctgcgcgagg cttcaatgac cgcctcctgc aggccggatt
     4981 cggccagact gcgcatgcag tcgggcatgg cgccctggcc gggcagcacc acgcgatcgg
     5041 ccgagcggat tgcctcgggc tgatcgacga tcgccacctc cgcttccggc gcggctttgc
     5101 gcagcgcctg ggccaccgag cgcaggttgc ccattccgta atccacaatc gctatcgaag
     5161 ttttcat
//
LOCUS       DATASH010000455        16130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1852740,
            whole genome shotgun sequence.
ACCESSION   DATASH010000455 DATASH010000000
VERSION     DATASH010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 16130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16130
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1852740"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1955
                     /locus_tag="VHV99_12600"
     CDS             <1..1955
                     /locus_tag="VHV99_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007740126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEX3636309.1"
                     /translation="LLLSRDRVALPAYDYCSMAVSAAERGDFRDSVEAAARALVVAQR
                     TDNADLAALSKRDLAIAYSYAGDLDDAQRYATEALASSPKSPEQVFAPSNKVLGDVAL
                     RRGHPLEALASYNRALETASARYKPLVLLSITNAQIAAGDTAGARKTFEAAGAPATAD
                     LAPEYRRIEGNLLLAEGKPEEARNAFSALLKEDNSGDANYDRLWAHEGIGRADLALGD
                     QGRAREAYLLAVDDSEKIRARFRSDEFKTGLFGDTQSVFETAIALTVGVGDYASAWNL
                     SERSRARALLDVVRNRVDAGVDDRQLNGDVPGLDVVRNALKPNEAIVEFHSLEKSTIV
                     WVIRNDGLKGYTLPIARTDMDSAVTDIRNAIMRRRQSAITYGDKLHALLIAPLGLRAN
                     ERLIIVPHGALHYLPFQALHSADGFLIQQHAIALEPSASVAVQLQQREQPVASNLVAF
                     GNPSISPPYALPGAEAEVRSIAPLFVRNEVFLQTSATRVSFRENAPAGRVLHVATHAE
                     ADTIDPLHSRILLAPATQPADGPDSLLAKDIYNLKLNNVSLVTLSACETGLGRIARGD
                     EILGFTRAFFYAGASSLIVSMWPVADESSALTMRTFYSQLADGKEAIDAMRAAQLAVL
                     QNPRFAHPFFWAPFDLMGGWRLTLAR"
     gene            1964..3640
                     /locus_tag="VHV99_12605"
     CDS             1964..3640
                     /locus_tag="VHV99_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007580051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HEX3636310.1"
                     /translation="MNKLRLIAAAVTCSSAFAWGQQLPNSGSLLNQTAPRDTRPPPPG
                     GANALPPVPQGTTPPVTAPGPTFMLKGITLKGNDTIPTDVLLAPVRDKIGTQIGFADL
                     TAIASRITQVYRERGYVLAQVLVPAQDVSSGNVELSVLEGRVGHVRLDIAAGTPIREN
                     LLRARVAQIPVGTPLQQHDLERTMLLLSDLPGIVVTSSIEAGEEPGTVDLTINVAPTK
                     RLTFAVDLDNYGATSSGTWRLGTLARLNSPFMLGDNLDIRLLAAERLDTFYGRVGYEA
                     PVNAQGTRVGVAVSRLNYALGKEFASLDAQGEATVVDFTVTHPLLRTRSQNLLARVNF
                     EYRDLIDNIGVVDSHNPRSMVEGSVGLSYESRDSFLGGGFNSADVEMLIGSLHFRNAA
                     AQELDASNFGRNTQGNEMRATFFANRLNAVTERVSVFAGVSGQWAATTLDNSSRFLLG
                     GPHAVRAYSPSEALVDEGFVATVEARYAVSSKATVFGFFDFGTGWYNANSRPNQGPNM
                     ITRSGVGVGGFWAAPGGFSLQGTVAWRTTGSDTTGDDKVPRLYVQLTKTF"
     gene            3771..9362
                     /locus_tag="VHV99_12610"
     CDS             3771..9362
                     /locus_tag="VHV99_12610"
                     /inference="COORDINATES: protein motif:HMM:NF017658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HEX3636311.1"
                     /translation="MNKSCRMRPSHAGTIAGAVAALFAASYAPAPLANPTGGQVVAGT
                     VTIASPTAGQMNITQTSPKGIVNWNTFSIGSSEAVNIVQPSSSAALLNRVMGNDPSVI
                     AGRLQANGKVFLVNPAGVVFARGASVNVGSMVASTLTISNDDFLAGNYHFIGTSAASV
                     TNAGTLTAGSGGTIALLGGSVSNSGTVSARMGTVAMGAGSDITLDFAGDGLTTLKIDQ
                     GAANALIGNTGTLAADGGMVVMSAQTADALAGTVINQQGIVRAQSIAERDGHILLDGG
                     SSGVTMAGGTLDATGGAGSTGGRIDVTGYNVALTDAARVDASGAAGGGVVRFGGGSAG
                     QDPTIRNANAIWMSPTAQIHADALASGNGGNVVAFSNTASRLYGTLTAKGGTQGGDGG
                     LIETSGHYLDMAEAKIDASAPMGKGGTWLIDPADIDICHATADGTCGVGAVIEVATFV
                     GSNDGAIAAPAIAALAVAGPVFGPATGAALTMVTDQQISTALNANTTVQVKTTDGNIT
                     MDGTETATATATGAAAIQKTQGAPSTLTLSASGSIIFNAGASITSTAAGGALNLVFDA
                     NVGNQTSANVIQSLGTLATPVVFSTVGGNLSMGVMAAQPGSFDGAAVQLAFTNIDTRI
                     NQSDTVPGGSVTIHGQAPGALPLTQTNVVGGTIPDLYAVGIFSSNIFTSTGAIELEGN
                     IGTGTQKPVGGVAVGAATPFADGVLPPANLQSTLSAGTGAIRIFGIGTDATGVSAYAV
                     AGARIFGGSNLLSNGGPIDIRGDVSGVAQGAFHDVGVVLGYSTINAAGANGSISLTGS
                     TNTSGTGVLLSSTLDNVESGGATMSTGQGGTITFRALNNGASDSFQFVGSASATSPGG
                     TLVFVPGGVNTQDFSLTRSDATPINVNSDAAGLSVDAAALAALVGEESGFNSIIFGST
                     TQTGLVTVQILTDCTSGDCISTRPTFSTNLTLASGGAGSQGIALPYGVSMPNLTLTVQ
                     SAGPVTQNGGIVANRILLDGPGRFTLSNAQNQVNSLAVVGTGDVTFVNASGLGIGITN
                     GSEAGPITGLTFDSATNQTTPISGANSTISGNLTVETTSGNITVWNPITKNAGSDATL
                     TLSAAGSIFDYANIVSTHGALNLVFDANVGNTISGNSISIQGLSPGDGLQAVPVDLFT
                     NGGNLSIGAKAGQTTSFDGAAAQLGYVNIDTRVSSPNGPTGNLSNGSVTIHGQAAGAV
                     NNYFNELYSVEILGSTINTATGNIDIDGSIGNGRPGSVGGVLLATAYLPALGSLPPAS
                     DISSLSTDSGSIHIYGAASDQGVSGTVDVDGVRAYAGTSIHSGSGNIDIRGDVVGASS
                     AKSNVFTGDFGVELGYSKITTTAGGSSISITGSTDTLDPGLLLSSTLNGVESGGPTIS
                     AGPNGVVALQAKNAGAADSIHFVGNAISVSSQNGTLSMAPGGVDPGSFSLTRSDTTPI
                     NIFSNGAGFSVSASALGFFSGFQTISFGSPTQTGLITVNGIAACSTCAPTRPNFSPNL
                     TLLSPGPGSQGISLPYGISMPRNALTLVSAGPVTDPGGIQAASLLLSGPGTFTLNDPQ
                     NAVGVLAMVNAGNVNFLNSGNLVIGPVTSQTYNPGTNTMQPLDGTNSSVTGNLVAQSA
                     TGNVQLNTNLSAGGSADLVAKNGVFTAAPSSTLTAGSGGWRIWTNTWVGETRHNVQPG
                     NVQPNFYGCTFGSGCSWGGTVPSTGNHYVYVQRPTLTVTADGKTRLAGAPNPTFTYSF
                     NGIVNGDSAADALNGTVTSPATKNSPAGTYPIDPNFASSVGYIVNDVPATLNVMPTTL
                     SPVAQSGLQTFFTSQEQSFVYENNLQGTNICVGVNQPLFTETPPGENQDLLAVEWKRV
                     RSQPNLNSCMTTNGQHGCSEF"
     gene            complement(9418..10218)
                     /locus_tag="VHV99_12615"
     CDS             complement(9418..10218)
                     /locus_tag="VHV99_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018423000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEX3636312.1"
                     /translation="MQVATASLTDQGGRPRNEDSFGDWSSERLYYCVVADGAGGHGGG
                     DTASRIVVDSVLADLRYLTVAELPPTGERLVSALAHANENIVEEQLRGGGKEKDMRST
                     AVLLAIDRECGTAAWAHCGDTRLYCFRHGEISARTRDHSMVQEMVDAQLLTAEAMRHH
                     PRRNVLYAALGTRDQLDIAGVDGTFALRDGDAFLICTDGFWEYADDACMGDALVEAAT
                     PGAWLDALAEKVRASASANHDNFTALAVWVSDDPEATVLQLAPGSSQQ"
     gene            complement(10253..10933)
                     /locus_tag="VHV99_12620"
     CDS             complement(10253..10933)
                     /locus_tag="VHV99_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636313.1"
                     /translation="MESIYTIVRHALTGYHPSELQALGEDKEELISQFLYLKVFRLET
                     AQTAVPNFPLHSAPSTSHAVCAYFRRYLIDCLRSASHQRNVSLDEGDVMLEIDRQTAF
                     HPDPIGSVLLQYGLNEAQVRESASHFIASLDDADRMLLAGSLGWLSNEKGGLSQVAAR
                     YGIASYHYRARKLGVTLKKGSIPADFARTEIGQWIEQRLGVDITPGNRIAILLVLGIL
                     SAQSHAQE"
     gene            11810..12844
                     /gene="tssA"
                     /locus_tag="VHV99_12625"
     CDS             11810..12844
                     /gene="tssA"
                     /locus_tag="VHV99_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HEX3636314.1"
                     /translation="MATLATGVMLAEISAASPCGDDLEYDPGFLELEQVVHGKPDVQY
                     GETMVAATPPDWKIAEALSLDLLGKSRDLRVAVHLSRALLNRKGFGGFAEGLALVEGL
                     LDQRWEHVYPQLDPDDDNDPTARVNALAALTEQSGMLVDVRDALLASSRVHGVVTLRD
                     IEYATGEVPPGNGAEAPSLNSIEAVIADAHEEVAATHAALLEALHSAMRIETVLTERV
                     GAARSIDLSPLARLLRRAADFLGERIASTGDTGGPVETADPSSGGGAAAGERTGAPLS
                     GDIADRQDVIRMIDKICAYYERHEPSSPVPLLLMRARRLVDKSFMEILQDLAPEGMGQ
                     ARQVGGIENE"
     gene            12937..13437
                     /gene="tssB"
                     /locus_tag="VHV99_12630"
     CDS             12937..13437
                     /gene="tssB"
                     /locus_tag="VHV99_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007740143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HEX3636315.1"
                     /translation="MAKESSQKFIARNRAPRVQIEYDVEVYGSEKKVNLPFVMGVLAD
                     LSGQPADPLAPVADRKFLEIDVDNFDERLKSMKPRVAVQVPNMLTGEGNLSVDMTFES
                     MDDFSPAAVARKVDALSKLLEARGQLQNLLTYMDGKTGAEDLIAKLLNDPALLQSLAS
                     APKPQE"
     gene            13463..14959
                     /gene="tssC"
                     /locus_tag="VHV99_12635"
     CDS             13463..14959
                     /gene="tssC"
                     /locus_tag="VHV99_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HEX3636316.1"
                     /translation="MVEPTTQGQAQPGEAALEVSDFANLLQKEFKPKSDKAKEAVETA
                     VRTLAEQALRDTNVISGDVLATIESLIAQIDAKLTAQINQIIHSEEFQKVESAWRGLH
                     YLVNNTETDESLKIRVMNVSKGDLHKTLKKYKGTAWDQSPFFKKLYEEEYGQFGGEPY
                     GALVADYHFDNSGPDVDLLTQMAKISAAAHAPFIAGADPAVMLMDSWQELANPRDLTK
                     IFQTPEHAAWRSLRESEDSRYVGLAMPRFLARAPYGAKTNPVEEFDFEEDTAGADSKK
                     YSWANAAYAMATNINRSFKRYGWCSRIRGIESGGAVENLPVHAFPTDDGGVDMKCPTE
                     IAISDRREAELAKNGFMPLVHKKNSDFAAFIGAQSLHKPAEYEDPDATANANLAARLP
                     YLFACSRFAHYLKCIVRDKIGSFKEKDDMQRWLQNWILQYVDGDPAHSTEESKARRPL
                     AAAEVVIEEVEGNPGYYTSKFFLRPHYQLEGLTVSLRLVSKLPSAKAA"
     gene            15004..15492
                     /locus_tag="VHV99_12640"
     CDS             15004..15492
                     /locus_tag="VHV99_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HEX3636317.1"
                     /translation="MAIDMFMKLGDIKGESQDKSHKEEIEVLAWSWGLSQSGTMHMGT
                     GGGAGKVSVQDLSFTKYSDKASPPIMAACTKGTHIETALLTVRKAGGDNPLEYYKITL
                     EGVLISSYSTGGSGGEERFTENVTLNFEKFHVEYQPQNAKGAKEGGVVEVKWNIPANA
                     DT"
     gene            15588..>16130
                     /locus_tag="VHV99_12645"
     CDS             15588..>16130
                     /locus_tag="VHV99_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system accessory protein TagJ"
                     /protein_id="HEX3636318.1"
                     /translation="MNAEEYLREGDLDGCLRELQNQVRKDPSKAAYRVFLFQLLAVTG
                     AWDRALTQLNVVGELDAAALPMVQTYREALQCEALREAVFKGERAPLIFGEPTGWLAL
                     LLEALRLDAAGDGDAAAAARAQALDEAPATAGALNGEPFAWLADADNRFGPTLETVVN
                     GRYYWIPFSRIKRIEIEPPSD"
BASE COUNT         3202 a         4876 c         5036 g         3016 t
ORIGIN      
        1 gtttgctgct ctcgcgcgac cgcgtcgcat tgcctgccta cgactactgc agcatggccg
       61 tctccgctgc tgagcgcggc gacttccggg atagcgtcga agcggcagca cgcgcgctcg
      121 ttgtcgcgca gcggaccgac aacgccgatc tcgccgcatt gtccaagcga gatctcgcga
      181 tcgcctacag ctacgcaggc gacctcgacg acgcccagcg ttacgcgacc gaagcgctgg
      241 catcgtcgcc gaaatcgccg gagcaggtct tcgctccctc caacaaggta ctcggtgacg
      301 tcgccttgcg gcgcggtcat ccgctggaag ccctcgcgtc atacaaccgg gcgctggaga
      361 cggcttcggc gcgctataaa ccgctcgtgc tgctctccat cacgaacgcg caaatcgcgg
      421 cgggcgatac ggctggcgcg cgcaagacgt tcgaagcggc cggtgcaccc gccacggctg
      481 accttgcgcc cgaatatcgg cgcatcgaag gcaacctgct gctggccgag ggcaagccgg
      541 aagaggcacg caatgcgttc agcgcgctgc tgaaggaaga caactcgggc gatgcgaact
      601 acgaccgcct gtgggcgcac gaaggcattg gtcgcgccga cctcgcgctg ggcgaccagg
      661 gacgcgcccg cgaggcctat ctgcttgccg ttgacgattc ggagaagatc cgcgcgcgtt
      721 ttcgcagcga cgagttcaag accgggctgt tcggtgacac gcagtcggtg tttgaaacgg
      781 cgatcgcgct tacggtcggg gtgggcgact acgcatccgc gtggaacctg tccgaacgca
      841 gccgggcacg cgcactgctg gatgtcgtgc gcaaccgcgt cgacgccggc gtagacgacc
      901 ggcaactgaa cggcgacgtg ccgggcctcg acgtggtccg taatgcgctg aagccgaacg
      961 aggcgattgt cgagtttcac agcctcgaga agtcgaccat tgtgtgggtg attcgcaacg
     1021 atgggctaaa gggttatacg ctgccgatcg cgcgcaccga catggattcg gccgtcaccg
     1081 atatccgcaa tgcgatcatg cgtcgccggc aaagcgcgat cacttatggc gacaaactcc
     1141 acgcgttgct gatcgcaccg ctcggtttgc gcgccaacga gcgactgatc atcgttccac
     1201 acggcgcgct gcactatttg ccgtttcagg cgttgcacag tgcggacggc tttctcatcc
     1261 agcaacatgc aattgcgctg gagccgtcgg cgagcgtggc cgttcagctc cagcaacgcg
     1321 agcagccggt ggcgagcaat ctggtcgcgt tcggcaatcc gtcgatctcg ccgccgtatg
     1381 cattgccggg cgcggaagcc gaagtgcgca gcatcgcacc gctgtttgtg cgcaatgagg
     1441 tgtttctgca gaccagcgcg acccgcgtga gctttcgcga gaacgcgccg gctggccgcg
     1501 tgctgcacgt cgcgacgcat gccgaagcgg acacgatcga cccgctgcat tcgcgcattc
     1561 tgctcgcgcc ggccacgcag cccgccgacg gccccgattc attgctcgca aaagatatct
     1621 acaacctcaa gctgaacaat gtgtcgctcg tcacgttgtc ggcctgcgaa acaggactcg
     1681 gccggattgc gcgcggcgat gagattctcg gcttcacgcg cgcgtttttc tacgcaggcg
     1741 cgtcgagcct gatcgtgtcg atgtggcctg tcgcggacga atcgagcgcg ttgacgatgc
     1801 gcacgttcta ttcgcaactg gccgacggaa aagaagcaat cgacgcgatg cgtgctgcgc
     1861 aactggccgt tttgcagaat ccacgctttg cacatccgtt cttctgggcg ccgttcgatc
     1921 tgatgggcgg gtggcgcttg acccttgcgc gctaacgaca agaatgaaca aactcagact
     1981 gatcgccgcg gccgtcactt gcagcagcgc gtttgcgtgg ggccagcagt tgccgaattc
     2041 aggcagcctc ctcaatcaga ccgcgccgcg cgacacgcgg ccgccgccgc cgggcggcgc
     2101 aaacgcgttg ccccccgtcc ctcagggcac cacgccaccg gtcaccgcgc cggggccgac
     2161 cttcatgctc aaggggatta ccctcaaggg caacgacacg attccgaccg acgtcctgct
     2221 tgcgcccgtg cgcgacaaga ttggcacgca aataggattt gccgatctca cggcgattgc
     2281 ttcgcgcatc acccaggtgt accgcgagcg cggttacgtg ctcgcgcagg tgctcgttcc
     2341 ggcgcaggac gtgagctccg gcaatgtcga actgagcgtg ctcgaaggac gtgtcggtca
     2401 cgtgcgtctc gacatcgccg cgggtacgcc gatccgtgaa aacctgctgc gggcgcgcgt
     2461 cgcgcagatt ccggtcggca cgcctttgca gcagcacgat ctcgagcgca cgatgctgct
     2521 tctatccgac ttgccgggca tcgtggtcac gtcgtcgatc gaggcaggcg aggaacccgg
     2581 caccgtcgat ctgaccatca acgtcgcccc caccaagcgc ttgacgttcg cggtcgatct
     2641 cgacaactac ggcgccactt cgagcggtac gtggcgtctg ggcacccttg cgcgcctgaa
     2701 ttcgccgttc atgctcggcg ataacctgga tatccggctt ctggcggccg aacgcctcga
     2761 taccttctat ggccgtgtcg gctatgaagc gcctgtcaac gcgcaaggca cgcgtgtcgg
     2821 agtcgccgtg tcgcggctca actatgcgct cggcaaagag ttcgcttcac tcgatgcgca
     2881 gggcgaggcg acggtcgtcg atttcacggt gacccatccg ctcttgcgca cccgcagcca
     2941 gaatctgctg gcgcgcgtga atttcgagta tcgcgatctg atcgacaaca tcggcgtggt
     3001 cgactcgcac aatccgcgct cgatggtgga aggcagcgtc ggactgagct acgaaagccg
     3061 cgactcattt ctcggcggcg gcttcaatag cgcggatgtc gaaatgctga tcggcagcct
     3121 tcatttcaga aacgcggcgg cgcaggaact cgacgcctcc aacttcgggc gcaatacgca
     3181 gggcaacgag atgcgcgcga cctttttcgc caaccggctc aatgcggtga ccgagcgggt
     3241 gagcgtgttt gccggcgtgt caggtcaatg ggcggccacg acgctggaca actcgtcgcg
     3301 cttcctgctg ggcggaccac atgccgtgcg tgcttactcg ccgtcggaag cccttgtcga
     3361 cgagggcttt gtcgcgacgg tcgaggcgcg ctatgcggtc tcgtcgaaag cgaccgtatt
     3421 tggtttcttc gatttcggca ccggctggta caacgcgaat tcccgcccga atcaaggacc
     3481 gaacatgatc acgcgcagcg gtgtcggcgt gggcgggttc tgggcggcgc cggggggatt
     3541 ctcgctacag ggcaccgttg catggcgcac gacgggatcc gatacgacgg gcgacgacaa
     3601 ggtgccgcgc ctgtatgtgc agttgacgaa gacattctag tgccgcaaaa cacgcaggac
     3661 gcagcggccc gcggttttga gtgcacatgc agttcaggcg cgtgaagaga tggcgccggg
     3721 actgttgaat cgtgtagcga gccggcatgt gcctggcatt gaggtgtgcg atgaacaagt
     3781 cctgccgtat gcgacccagc catgccggca ccatcgcggg cgcggtggcc gcgttattcg
     3841 cggcgtcgta cgcgcccgcg cccctggcga acccgacggg cgggcaggtg gtggcgggga
     3901 cggtgaccat cgcttcgccc actgcggggc agatgaacat cacacaaacc tcgccgaagg
     3961 ggatcgtcaa ctggaacacc ttctcgatcg gctcgagcga agcggtcaac atcgtccagc
     4021 cttcgtcatc ggcggcgctg ctcaaccgcg tgatgggcaa cgatccgagt gtcatcgcgg
     4081 gacgcttgca ggcgaacggc aaggtgtttc tcgtcaatcc ggccggcgtt gttttcgcgc
     4141 ggggggcgtc ggtgaatgtc ggctcgatgg tggcgtcgac gctcactatc tccaacgacg
     4201 attttctcgc cggcaactat cactttatcg gcacgtcggc ggcatccgtc acgaacgctg
     4261 ggacgttgac ggctggctcg ggtggaacga ttgcgctgct gggtggcagc gtgagcaatt
     4321 cggggaccgt gagcgcgcgc atgggcactg ttgcgatggg cgccggcagc gacatcacgc
     4381 tggacttcgc gggtgatggg ttgacgaccc tgaagatcga ccagggcgcg gcgaacgcgc
     4441 tgatcggcaa tacgggcacg ctggcggccg atggcggcat ggtggtcatg agcgcgcaga
     4501 cggccgatgc gttggccggc accgtcatca accagcaggg aatcgtgcgg gcgcagagca
     4561 ttgccgagcg cgacggacac attctgctcg atggcggcag cagcggtgtg acgatggcgg
     4621 gcggcacgct cgatgcgacg ggtggcgcgg gatcgacggg cggccggatc gatgtgaccg
     4681 gctataacgt cgccttgacg gatgctgcgc gagtcgacgc gagcggcgcg gcgggcggtg
     4741 gcgtcgtgcg gtttggcggc ggttcggcgg gacaggatcc gacgattcga aatgccaatg
     4801 cgatctggat gtcgccgact gcgcagatcc atgcggatgc gctggcgagc ggaaatggcg
     4861 gcaacgtggt cgcttttagt aatacggcta gccgcctata tgggacgctc acggccaaag
     4921 gcgggacgca agggggggat ggtgggttga tcgagacgtc ggggcactac ctcgatatgg
     4981 cggaagcgaa gatcgatgcg tcggcgccga tgggcaaggg tggcacgtgg ctgatcgatc
     5041 ccgcggacat cgacatttgt catgcgaccg ctgatggcac gtgtggcgtc ggggcagtga
     5101 tagaagtagc gacatttgtc ggcagtaacg acggcgccat tgcggcgccc gccattgcgg
     5161 ctctcgcggt tgcgggtccc gtgttcggcc cggcgaccgg ggcagcgctg acgatggtaa
     5221 cggatcagca aatcagcact gcactcaacg cgaatacgac cgtgcaggtc aagacaacgg
     5281 acggcaacat tacgatggat gggaccgaga ccgcgaccgc taccgctacc ggcgccgcgg
     5341 ccatccagaa gacgcagggc gccccgtcga cgctcacgct gagcgcgtca ggatcgatca
     5401 tcttcaatgc cggcgcaagc atcacgtcga cagcggccgg cggcgcgctc aatctggtat
     5461 tcgacgccaa tgtcggcaat cagaccagtg caaacgtgat tcaaagcctg ggcaccctgg
     5521 caacgccggt tgtcttttcc acggtgggtg gaaacctcag tatgggcgtg atggcggcgc
     5581 agccaggttc gttcgatggc gcggccgtac agcttgcatt cacgaacatc gatacccgga
     5641 tcaaccagtc ggacaccgtg ccgggcggtt cggtgacgat tcatgggcag gccccaggag
     5701 ccttgccact cacccaaacg aatgtggtag ggggaacgat tcctgatctc tacgccgtcg
     5761 gcattttcag ctcgaacatt tttacctcga cgggcgcgat cgaactggaa ggcaatatcg
     5821 gcactgggac acaaaaacca gtgggcggag tcgcagtggg tgcggcaaca cccttcgcgg
     5881 acggcgtact gccgccagcg aatcttcagt cgacgctgtc ggctggcacg ggcgccatca
     5941 ggatattcgg tatcggtaca gacgctacag gggtatctgc ttacgctgtt gccggcgcgc
     6001 ggattttcgg aggctcgaac cttctttcga acggaggccc catcgatatc cggggagatg
     6061 tgtcgggtgt tgcgcaaggc gcatttcacg acgtcggtgt cgtgctaggt tactcaacaa
     6121 tcaacgccgc tggagcaaac ggatcgattt cgttgactgg ctcgacaaat acgagtggga
     6181 cgggtgtgct cctcagcagc acgctggaca acgtcgaaag cggcggtgca acgatgtcga
     6241 cggggcaggg agggaccatc acgtttcgcg cgctcaacaa tggagccagc gacagcttcc
     6301 agttcgtcgg gagcgcaagc gcgacctcgc caggaggcac acttgtgttc gtcccgggcg
     6361 gggtgaacac tcaggatttc tccctcaccc gatcagacgc caccccgatc aacgtcaata
     6421 gcgatgccgc aggccttagc gtggatgcag ccgcactcgc agcgctggtt ggcgaggagt
     6481 caggtttcaa cagcattatt ttcggcagca cgacgcagac cggactcgtg acggtgcaga
     6541 ttctcaccga ctgtacgagc ggtgactgca tctccacgcg tcccacattc tccacgaatc
     6601 tgaccttggc ctccggcggc gcaggcagtc aaggcattgc gctgccctac ggcgtgtcga
     6661 tgcccaacct cacgctcacc gtgcagtcgg ccggccctgt gacccagaac ggcggtattg
     6721 tggcgaaccg gattctgctc gatggaccgg gcaggttcac cttgagcaat gcacagaatc
     6781 aggtgaacag tctcgcggta gtcggcaccg gcgacgtgac cttcgtgaac gcatccgggc
     6841 ttggaatcgg tataaccaat ggatccgaag ccggtccgat cacaggtctg acgttcgatt
     6901 cggccacgaa tcagacgacg ccgatctcag gtgccaactc cacgatcagc ggcaatctga
     6961 ccgttgagac gacgagcggc aacatcacgg tgtggaatcc gatcaccaag aatgccggta
     7021 gtgacgcgac gttgacgctg agcgcggcag gttcgatatt cgactatgcg aacatcgtat
     7081 cgacccatgg cgctcttaac ctcgtgttcg atgcaaatgt aggtaacacc atcagcggca
     7141 attccatttc gatccagggg ctctcgccgg gggacggcct ccaggccgtt ccagtcgacc
     7201 ttttcaccaa tggcggcaac ctgtccatcg gggccaaggc cgggcagaca acctcgttcg
     7261 atggcgcggc cgcgcagctc gggtatgtca acatcgatac ccgggtgagc tcgccaaacg
     7321 ggcccaccgg caatctctcg aatggctctg tcacgatcca tggtcaagcc gcgggagcgg
     7381 taaacaacta ctttaacgaa ctctattcgg tcgaaattct cggttcaaca atcaacacgg
     7441 cgacgggcaa catcgacatc gatgggagta ttggcaatgg ccgtcctgga tccgtcggcg
     7501 gtgtattgct ggcaacggcg tacctgccgg cgctcgggtc actgcctccc gccagtgaca
     7561 tttcgagcct gtcgacggac agcggctcga ttcacatcta cggcgcggct tcggaccaag
     7621 gcgtgtcggg cacggtggac gttgatggcg tgagagctta cgcaggaact tcaattcata
     7681 gtggaagcgg gaatatcgat attcgcggtg acgttgtcgg cgcttcttcg gcaaaatcga
     7741 atgttttcac gggcgatttc ggtgtcgaat tgggctattc gaagatcacc acaacggctg
     7801 gcggaagttc gatctcgatc acggggtcga ccgacacact ggatcctggg ctgttgcttt
     7861 cgagcacatt gaatggcgtt gaaagcggtg gaccgactat cagcgcgggc ccgaacggcg
     7921 tagttgcact gcaagccaaa aacgcaggcg cagcggatag cattcatttc gtcggaaacg
     7981 ctatttccgt ctcgtcgcaa aacggtacct tgagcatggc gccgggcgga gtcgaccctg
     8041 ggtcgtttag cctgacccgc tcggacacta cgccaatcaa tattttcagc aacggtgcgg
     8101 gcttcagtgt cagcgcgagc gcgctcgggt ttttctccgg ttttcaaacg ataagttttg
     8161 gaagcccaac gcagactggt ttgatcacgg tgaacggcat cgctgcatgt agtacctgcg
     8221 ctccgaccag gccaaacttt tctcccaatc tgacactatt gagccctgga ccaggaagtc
     8281 agggaatcag cttgccgtac ggcatctcga tgccacgcaa tgctctgacc ttggtgtccg
     8341 ccggtcccgt caccgacccg ggtggcatcc aggcggccag tctgctgctg tccggcccag
     8401 gcacgttcac cttgaacgat ccgcaaaacg cagtcggcgt actcgcgatg gtcaatgcgg
     8461 gcaatgtgaa tttcctcaac tctggaaatc tggtcattgg cccggtcacc agtcagacct
     8521 ataacccggg cacgaacaca atgcagccac tcgacggcac aaattcgagt gtaaccggca
     8581 acctcgtcgc ccagtcggcc accggcaacg tccaactcaa caccaacctg agcgctggcg
     8641 gcagcgccga tctggtcgcg aagaacggcg tgttcactgc cgcgccttcc agtacgttga
     8701 cggccggaag cggcggctgg cgaatctgga cgaacacatg ggtaggagaa acccgtcaca
     8761 acgtccaacc gggcaacgta cagcccaatt tctatggctg tacgttcggt tcaggctgta
     8821 gttggggcgg cacggtaccg tcgacaggga accactacgt gtacgtccaa cggcctacgc
     8881 tgacagtcac cgccgacggc aaaacacggc tcgccggcgc gcccaatccg accttcacct
     8941 atagcttcaa tgggatcgtg aacggcgatt ccgccgcgga tgcattgaac ggaaccgtta
     9001 cctcgcccgc tacgaagaat tctcccgcgg gtacgtatcc gatcgatccg aatttcgcat
     9061 cgagtgtggg ctatatcgtg aatgacgttc ctgcgacgct gaatgtcatg ccgaccacgc
     9121 tttctcctgt tgcgcagagt ggcttgcaaa ccttctttac cagccaggaa cagtccttcg
     9181 tctacgagaa caacttgcaa ggcaccaaca tttgcgtcgg ggtgaatcag cctctcttca
     9241 ccgaaacgcc tccgggcgag aaccaggatc ttctcgccgt ggaatggaag cgggtgcgct
     9301 cgcaaccgaa tctgaacagc tgcatgacca cgaacggtca acacggttgc agtgaattct
     9361 agcccgcacc cactaacgcg tgcttcgccg gctgcctgaa tccgccgtga cggtgcgcta
     9421 ttgctggctg gaacccggcg cgagctgcaa caccgtcgcc tccggatcat cgctcaccca
     9481 caccgcgagc gccgtgaagt tgtcatgatt cgcgctggcg ctcgcgcgta ccttctccgc
     9541 cagcgcgtca agccacgcgc cgggtgtcgc ggcctcgacc agcgcgtcgc ccatgcaggc
     9601 gtcgtcggca tattcccaga agccatcggt gcagatcaga aaggcgtcgc cgtcacgcag
     9661 cgcgaacgta ccgtccacgc ccgcaatgtc cagttgatcc cgggtgccca gcgccgcata
     9721 cagcacatta cggcgcgggt gatggcgcat cgcctcggcg gtgagcaact gcgcgtcgac
     9781 catctcctga accatgctgt ggtcgcgcgt ccgggcgctg atttcaccat ggcggaaaca
     9841 gtagagccgg gtgtcgccac aatgagccca ggcggccgtc ccgcattcgc gatcgatggc
     9901 gagcagcacg gctgtcgaac gcatgtcttt ttccttgccg ccgccgcgca actgttcttc
     9961 gacgatgttc tcgttggcat gcgccagcgc gctcaccagc cgctcgcccg tcggcggcaa
    10021 ttcggcaaca gtcaggtagc gcaaatccgc cagcacggaa tccacgacga tgcgcgaagc
    10081 ggtgtcgccg ccgccgtgtc cgccggcgcc gtccgcgacg acgcaatagt agagccgctc
    10141 gctcgaccag tcgccgaacg aatcttcatt gcgtggccgg ccgccctggt cagtcaggct
    10201 tgcggtggca acttgcaccg ttgacctctc atgtgctcgt gatctttcga cctcattcct
    10261 gcgcgtgcga ctgcgcggac agaatgccca gaaccagcag gatggcgatg cgattgcccg
    10321 gtgtgatgtc gacgcccagg cgctgctcga tccattggcc gatttccgtg cgggcgaagt
    10381 cggcgggtat cgagcctttt ttcaaggtga cgccgagctt gcgcgcccgg tagtgatatg
    10441 acgcgatgcc atagcgcgcc gccacctgcg acaaaccgcc tttttcgttt gacagccagc
    10501 cgagactgcc cgcgagcagc atgcggtcgg cgtcgtcgag cgaggcgatg aagtgcgacg
    10561 cggattcgcg cacctgggcc tcgttcaatc cgtattgcag cagtaccgag ccgatcggat
    10621 caggatggaa tgccgtttgc cggtcgattt cgagcatcac gtcgccttcg tcgagcgaca
    10681 cgttgcgttg atggctcgca ctgcgcagac agtcgatcag atagcggcga aagtacgcgc
    10741 acacggcatg actggtcgaa ggcgcactgt gcaacgggaa gttcggcact gccgtctgcg
    10801 cggtctcgag acggaatacc ttcaaataga ggaactgcga gatcagctct tccttatcct
    10861 cgccaagcgc ctgcagttcc gacggatggt agcccgtcag cgcgtggcgc acgatcgtat
    10921 agatcgattc catctcgtca tggctcaaac aggtgcgccg ttgccacaga tcgggcaatg
    10981 cgctttcccc agcatggtct atcgctgctg cgccttggat atgtgccact tgtcaccccg
    11041 aagtggtgag ccccgcgtcc ggggcgaatc cctgctgctc acactgccat ttttaccgct
    11101 tctgtggtgc agcggattcc agtcgccaat gaatcctgaa cccgcccccg cctccccgat
    11161 aagaaggcgc cgacgccaga cgcgcgcaat acccggaaat ccgccatgca tccggaagag
    11221 acatcaaaaa caaagttagt ctattggcct gttttattta ttacaagata aaacttaata
    11281 tgtgatacgg atcaattcat atgcgcgttc cattgatatc ggtattaacc cttaaattta
    11341 tgtcagggtt tacctgcttg gtcgaggtgt gagatttttt aaaaatggag cgcctgattc
    11401 gtttacttat atgttcgacg attcgctgta tcgtccatcg gacaggcttg tcagagtagt
    11461 ccgaagatga caggaatcat cggacaatta agaattattg agcgacttac tagtcttaac
    11521 gttttaaata attagctaat tttttggtaa tacatatagt gtttcgctta attaaggaaa
    11581 ctccattttt attgaggtcc ggctttaatt ttccgggctg cgtgctattc tcatatcgga
    11641 ttgccggtaa gaaagtccga cgtgcgctaa aacatcgaaa tacccgcgca taggcaatct
    11701 aattagagtt gacacgcatt tccggggagc gctgattcat tgctggcaag gcaacccgta
    11761 ccggtcattt acggtactca agattactca tccgagaccg attcaagaca tggccacgct
    11821 tgctacgggt gtaatgcttg ccgaaatatc cgctgccagt ccgtgcgggg acgacctcga
    11881 gtacgaccct ggctttcttg aactggagca ggtcgtacac ggcaagccgg acgtgcagta
    11941 tggcgaaacc atggtcgccg ccacgccgcc cgactggaaa atcgcggaag cgttgtcgct
    12001 cgatttgctg gggaaaagcc gcgacctgcg tgtcgcggta catctgtcgc gcgcgctgct
    12061 gaaccgcaag ggtttcggcg gtttcgccga aggcctcgcg ctcgttgaag gattgctgga
    12121 ccagcgttgg gagcacgtct atccgcaact cgaccccgac gacgacaacg atccgaccgc
    12181 ccgcgtcaac gcgctcgccg cgctcaccga acagtccggc atgctggtcg acgtgcgcga
    12241 tgcactgctc gcgtcctcgc gcgtgcatgg tgtcgtcaca ctgcgcgaca tcgaatatgc
    12301 gaccggagaa gtcccgcctg gcaacggcgc cgaggcgccg tcgctgaact cgatcgaggc
    12361 ggtgatcgcc gacgcgcacg aagaggtcgc cgcgacgcac gcggcattgc tcgaagcgct
    12421 gcatagcgcg atgcgcatcg agacggtgtt gaccgaacgc gtcggtgcag cgcgttcgat
    12481 cgacctgtcg ccgctcgcgc ggctgctgcg gcgggcagcc gattttctcg gcgagcgtat
    12541 cgcgagtacg ggcgacaccg gcggcccagt cgaaactgcg gacccgtcat caggcggcgg
    12601 cgctgccgct ggcgaacgca cgggcgcacc gctgtccggc gacattgcgg accgtcagga
    12661 cgtgatccgc atgatcgaca agatctgcgc gtactacgag cgtcacgaac cctccagccc
    12721 ggtaccgctg ttgctgatgc gcgcgcgccg cctggtcgac aagagtttca tggaaattct
    12781 gcaggacctt gcgcccgaag gaatgggcca ggcacggcag gtcggcggta tcgagaacga
    12841 gtaagtagtc aggcggggat gtgctttttg cttttagcaa cgccagtcac gcgccacgct
    12901 gctcgcggcc gtgcggcgtc aaccagggga agggtcgtgg ccaaggaaag cagccagaaa
    12961 ttcatcgcgc gcaatcgagc gccgcgcgtt cagatcgagt acgacgtcga ggtctacggt
    13021 tcggagaaaa aggtcaatct gccctttgtc atgggcgtgc tggccgacct ctccggtcag
    13081 ccggcggatc cgctggcgcc ggtggcggat cgaaagtttc tcgagatcga cgtcgacaat
    13141 ttcgacgagc gtctgaaatc gatgaaaccg cgggtcgccg tgcaggtgcc gaacatgttg
    13201 accggtgagg gcaatctgtc ggtcgacatg accttcgaga gcatggacga cttttctccg
    13261 gcggcggtcg cgcgcaaggt ggacgcgttg tcgaagctgc tggaggcgcg cggccagttg
    13321 caaaacctgc tgacctatat ggacggcaag accggcgccg aggacctgat cgcgaaactg
    13381 ctgaacgacc ctgcgttgct gcagtcgttg gcatccgcac caaagccgca ggaatgagcg
    13441 tgatttacag gggagtctga ccatggtcga acctaccacc caagggcaag cgcaaccagg
    13501 cgaagcggcg cttgaagtga gcgatttcgc gaacctgctg caaaaggaat tcaaaccgaa
    13561 gagcgacaag gcgaaggaag ccgtcgagac ggcggtgcgc acgctcgccg aacaggcgct
    13621 gcgcgacacc aacgtgattt ccggcgatgt gctcgccacc atcgaatcgc tgatcgcgca
    13681 gatcgacgcg aaactcaccg cgcagatcaa ccagatcatc cactcggaag agttccagaa
    13741 ggtcgagagc gcgtggcgcg gcttgcacta tctggtgaac aacaccgaga cggacgagtc
    13801 gttgaaaatc cgcgtgatga atgtctcgaa gggcgatctg cacaagacgc tcaagaaata
    13861 caagggcacc gcgtgggatc agagtccctt cttcaagaag ctctacgaag aggaatacgg
    13921 ccagttcggc ggtgagcctt atggcgcgct ggtggccgat taccacttcg acaacagcgg
    13981 tcccgacgtc gatttgctga cgcagatggc gaagatttcc gccgcggcgc atgcgccgtt
    14041 catcgctggc gccgaccccg cggtgatgct gatggattca tggcaggaac tggccaatcc
    14101 gcgcgacctg acgaagattt ttcagacgcc tgaacacgcg gcgtggcgct cgctgcgcga
    14161 atcggaggat tcgcgctacg tcggtctcgc catgccgcgc ttcctcgcgc gcgcgcccta
    14221 tggcgccaaa accaatccgg tcgaagaatt cgatttcgag gaagacaccg ccggcgcgga
    14281 cagcaagaag tactcatggg ccaatgcggc gtatgcgatg gcgaccaata tcaaccgctc
    14341 gttcaagcgg tatggctggt gctcgcggat tcgcggcatc gagtcgggcg gcgcggtcga
    14401 gaacctgcct gtgcatgcgt tcccgaccga cgacggcggc gtcgacatga aatgcccgac
    14461 cgaaatcgcc atcagcgatc gccgcgaagc ggaactggcg aagaacggct tcatgccgct
    14521 cgtgcacaag aagaactccg acttcgctgc ctttatcggc gcgcagtcgc tgcataagcc
    14581 ggccgagtac gaagacccgg acgccactgc gaacgcgaat ctggccgccc gtttgcctta
    14641 cctgttcgcc tgcagccgct ttgcgcatta cctgaagtgc atcgtgcgcg acaagatcgg
    14701 cagtttcaag gagaaggacg atatgcagcg ctggctgcag aactggatcc tgcaatacgt
    14761 cgatggcgat ccggcgcact cgacggaaga gtcgaaagcg cgccggccgc ttgccgccgc
    14821 ggaagtggtc atcgaggagg tcgaaggcaa tccgggctat tacacgtcga agtttttcct
    14881 gcgtccgcac taccagttgg aaggcctgac cgtatcgcta cggctggttt cgaagcttcc
    14941 gtccgccaag gcggcatgac ggaaatcccc attcagtgac gattgttttg agcggagaaa
    15001 gacatggcca tcgatatgtt catgaagctg ggcgatatca agggcgagtc gcaggacaag
    15061 tcccataagg aagaaatcga agtgctggcg tggagctggg gcctgagcca gagcggcacc
    15121 atgcacatgg gcacgggcgg cggcgccggc aaggtgtcgg tgcaagacct gtcgtttacc
    15181 aaatacagcg acaaagcgag tccaccgatc atggcggcct gcaccaaagg cacccacatt
    15241 gaaacggcct tgctgaccgt gcgcaaggcc ggcggcgaca atccgcttga gtactacaag
    15301 atcaccctgg agggtgtgct gatcagttcc tattcgacgg gcggttcggg cggcgaagag
    15361 cgtttcaccg agaacgtcac actcaacttc gagaaattcc atgtcgaata ccagccgcaa
    15421 aacgccaagg gcgcgaagga gggcggggtg gtcgaagtga aatggaacat ccccgccaac
    15481 gcggatactt gatcggctga gcgcaaatgg cgactgcact gcggcggcgc tgccgtcgca
    15541 gtgcggcggc gggtgggacg actcaatcca gaccggagcg aactggtatg aacgcagaag
    15601 aatatttgcg cgaaggcgat ctcgacggat gtctgcgcga attgcaaaat caggttcgca
    15661 aggatccgtc gaaggccgcg tatcgcgtct ttctgtttca gttgctcgcg gtgacgggcg
    15721 cctgggatcg cgcactgacg caactgaacg tcgtcggcga actcgacgcc gccgcgttgc
    15781 cgatggtgca aacctatcgt gaagcattgc aatgcgaggc gctgcgtgaa gcggtgttca
    15841 aaggcgagcg cgcgccgctg attttcggcg agccgaccgg ctggctcgcg ttactgctcg
    15901 aagcattacg gctcgatgcc gccggcgatg gcgacgcggc ggcggccgcg cgcgcccagg
    15961 cgctcgacga ggcgcccgcc accgccggcg cgttgaacgg cgagccgttc gcgtggctcg
    16021 ccgacgccga caacaggttc ggcccgaccc tcgaaaccgt ggtcaacggc cgctattact
    16081 ggattccttt ttcgcgcatc aaacggatcg aaatcgagcc gccatccgac
//
LOCUS       DATASH010000456         2860 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1061758,
            whole genome shotgun sequence.
ACCESSION   DATASH010000456 DATASH010000000
VERSION     DATASH010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1061758"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VHV99_12650"
     CDS             <1..210
                     /locus_tag="VHV99_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636319.1"
                     /translation="QEGDTLIIPVYGYVPVVTMQLTLKEEVRVTTTGSVEQSLQNIRL
                     HNEEVVIERRKGPDGEWQREDPPQA"
     gene            334..1176
                     /locus_tag="VHV99_12655"
     CDS             334..1176
                     /locus_tag="VHV99_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008346666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsnF/AvaK domain-containing protein"
                     /protein_id="HEX3636320.1"
                     /translation="MSDAQRASDRLAQEGIARTDLHVHAQDASRESEVPDDVSGSGGT
                     PTRTVAADAEQGAPHEGGIAHFFKRLFGNDDAPEEAGHYRESVRRGHALLSVDVVDES
                     QIDAVRAAMNDAGAMDIEERVTQWKAAGYSGYDSSAPAYTADEIAEDRRTFPVVREDL
                     TIGRREVSTGGVRVYSRVTETPVSESVNLREEHATIERRTVDRPATEADRKEGAVEIR
                     ETAEEPVVAKTARVVEEVVVGKESSQRTETVNDTVRGTQVEVERDAGNAATGGADPVN
                     RKPL"
     gene            1763..2614
                     /locus_tag="VHV99_12660"
     CDS             1763..2614
                     /locus_tag="VHV99_12660"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX3636321.1"
                     /translation="MQQTFSPVRRFKKNTSGRDFVVGDLHGCFTQLRKELEAGQFDPS
                     RDRIFAVGDLVDRGFESPAVLDVVRRYGIQSVRGNHEDSIVRWRLYGASDGFVRRNGG
                     RWLLDMTGDEQSIDAIVAYMAALPYAIEIETDFGLVGIVHANVPLKSWPRTVEALLKE
                     GIDGKIRRKVIWDRSRWKLPEPGGFGSMRRVAARLFRRLRSARLDTSGWVEGVAAVIV
                     GHTPSSGPIVSHNVINVDTGVVYGGNLTLLRLDEIPTLLNQGTGAAPAFSRITQCSPA
                     RKSEQLQ"
BASE COUNT          608 a          859 c          889 g          504 t
ORIGIN      
        1 caggaaggcg acacgctcat catcccggtg tacgggtacg tgccggttgt cacgatgcag
       61 ctgacgctga aggaagaggt tcgcgtgacg accaccggga gcgtcgagca gtcattacag
      121 aacatccgcc tgcacaacga ggaggtagtg atcgagcgtc gcaaaggccc tgacggggaa
      181 tggcagcgcg aggacccccc tcaggcgtga gccggcggga acggccgttg cgcaaacaga
      241 aagccatctc aggccccttc ggcgcatagc gccttcatcc tggtattctg gagaaatgtt
      301 atgactcaga ctctcgttgg gctgttcgat tccatgtccg acgcgcaacg cgcatcggac
      361 cgtctggcgc aggaaggcat cgcccgcacc gacttacacg ttcacgcgca ggatgcaagc
      421 cgtgaaagcg aagtgccaga cgacgtttca ggcagcggcg gcacgcccac ccgcaccgtc
      481 gcagccgacg ccgaacaagg ggcgccccac gaaggcggca ttgcccattt tttcaaaagg
      541 ctgttcggta atgatgacgc acccgaagag gcaggccact atcgcgaatc cgttcgccgc
      601 gggcacgcgc tgttgagcgt ggatgtggtc gacgaatcgc agatcgacgc cgtgcgggca
      661 gcgatgaacg acgccggtgc gatggatatc gaagagcgcg tgacccagtg gaaagccgca
      721 ggatatagcg gctacgacag cagtgccccg gcatacaccg cagacgaaat tgccgaagac
      781 cgcaggacgt ttcctgtcgt ccgggaagac ctgaccatcg gcaggcgcga ggtgtcgacg
      841 ggcggggtac gcgtctattc gcgcgttacc gagacgcctg tctccgaaag cgtcaatctg
      901 cgcgaagagc atgcgacgat cgagcgccgg accgtcgatc gccctgccac agaagcggac
      961 cggaaggaag gcgccgtgga aatccgcgag acggccgaag agccagttgt tgcaaaaact
     1021 gcgcgggtgg tcgaggaagt ggtcgtcggc aaggagtcca gccagcggac ggagaccgtg
     1081 aatgataccg ttcgcggcac gcaagtggag gtcgagcgcg atgcgggcaa cgcggccacc
     1141 ggcggcgccg atccggtgaa caggaagcca ctgtgagacc gggcccgcca gatcacggaa
     1201 cggcggtcag ctgactgccg ttttcccgtc ggccgcagcg cagacgccgc gagatgcaat
     1261 ttacttcgtt gctcaactgt caagcaggcc acgccctggg cggcagttgg accgcgcttg
     1321 taactgacag taaatatgga aaccgagacc tacaggtcgg gctggcgacc agacctcgcg
     1381 cgacgcgcct ttacgggcag aaacgacgcc ccttgatcga tcggacaaat accgccgttt
     1441 cctcaggcac acatgttgtt acaaacggat acgcctgcgg gcaatggaca ttcgacacta
     1501 cttctatggc ggccttacgc gccacgctgg caaacgcgca acatcagcat cggattgacg
     1561 ctaccgtctg ccggccggcg aagcaacgct cttgcaggcg cgcacgtggt gcgccgtgcg
     1621 cccgtgagta acccgctctg cactggcgaa gaacagcggg caaaactgcg ggccgtcgcg
     1681 ctgaaaattc gcgttggaca ctgcgatcca cttcgtccct tccacgaggg tacaacgtgg
     1741 acatccctac ggcggcaaaa aaatgcagca gaccttctca ccggttcggc gctttaaaaa
     1801 aaatacgtca ggtcgtgatt ttgttgtagg cgatctgcac ggatgtttca cccagctgcg
     1861 aaaagaactc gaagctggcc agttcgaccc gtcccgggac cggatctttg cggtcggcga
     1921 cctggtagac agagggttcg aatcgcccgc tgtcctcgac gtggtgcgtc ggtacggcat
     1981 ccagtcggtg cgcggcaatc atgaggactc gatcgtacgc tggcgtcttt acggcgccag
     2041 cgacggcttc gttcggcgca atggcggcag gtggctgctg gatatgacag gagacgaaca
     2101 gtccattgat gctatcgtcg cgtacatggc agccctcccc tacgcaatcg aaatcgagac
     2161 ggacttcggc ctggtcggga tcgtgcatgc caacgtgcct ctgaaatcgt ggcccagaac
     2221 ggttgaagcg ctactgaaag aagggattga cgggaaaata cggcgcaagg tcatctggga
     2281 tcggtcacga tggaagctgc cggaaccggg tggcttcggg tcgatgcgcc gcgtcgccgc
     2341 gcgactgttc aggcggctca ggtctgctcg gcttgatacc agcggctggg tcgaaggggt
     2401 ggcggctgtc atcgtaggac atacgccttc atctgggccg atcgtgagcc acaatgtgat
     2461 caatgtcgac acgggtgttg tgtacggcgg caaccttaca ctgctgcgcc tcgatgaaat
     2521 ccccacacta ctgaaccagg gcaccggcgc ggcacctgca tttagccgga taacgcaatg
     2581 ctcaccggcc cgcaaatctg agcaactaca atgaccacaa acgtatccgg gttcatgtgt
     2641 caccatgact gacgggcgcc ggcaaacccg ggcgcccaat tcaccggtgc gcgtcattcg
     2701 cccgatctga catttttcgc gggaacgcca aagcgcccgg ctgactcgtc tcattttctg
     2761 gctggtgtgc ctatccatct ggcttcgtca ccaagcctgc cgccttcttc aacgtctttt
     2821 ttatcccgat ctgtttcttc agaagcccgc ggacgccaca
//
LOCUS       DATASH010000457         2825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_467079,
            whole genome shotgun sequence.
ACCESSION   DATASH010000457 DATASH010000000
VERSION     DATASH010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467079"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="VHV99_12665"
     CDS             <1..443
                     /locus_tag="VHV99_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEX3636322.1"
                     /translation="AGGGVLLTDGSDAFFKAVEAKMSAPDLPLFAKGLLCNWLVCLAI
                     WMAARTTNDGAKLGLIFWPIFAFVASGFEHSVANMFAFALALMADHPASITLAGAIHN
                     EFFVTAGNLAGGAIFMALGYWLQDGSVGHASQARVPASERARTN"
     gene            complement(789..1787)
                     /locus_tag="VHV99_12670"
     CDS             complement(789..1787)
                     /locus_tag="VHV99_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX3636323.1"
                     /translation="MTIETIAIEGLTTPVSRIGLGTWAIGGWMWGGTDEKDSIAVIRR
                     ALDSGVNLIDTAPVYGFGASEEIVGKALADGYRHKAVIATKVALEWHDGAVFRNSSPE
                     RIRREVEDTLRRLRTDYIDLYQVHWPDPLVPIEETAAVLDDLRKEGKVRALGVSNFSP
                     AQMDAFRSVAPLSATQPPYNLFERDIDRDVLPYAKQHGLVALAYGALCRGLLAGRIGV
                     ETRFEGDDLRQRDPKFCEPRREQYVAAVKALNVFARDNFDKSVLALAVRWVLDRGPTI
                     ALWGARRPAQLDGIEDVFGWRLDEKAFKQIDAIVAQFVKDPVGPEFMAPPERVLAA"
     gene            2124..2276
                     /locus_tag="VHV99_12675"
     CDS             2124..2276
                     /locus_tag="VHV99_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636324.1"
                     /translation="MIGFGIAVQLADIRRATLLFVGLTTVLVLIALLLLMRGQAKREP
                     ERQSDR"
     gene            2292..2447
                     /locus_tag="VHV99_12680"
     CDS             2292..2447
                     /locus_tag="VHV99_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636325.1"
                     /translation="MLDAYRWHYIVSGVTQPRFSKLLYRMITPAQAEHIGDALAPIVQ
                     RPSDIDA"
     gene            complement(2505..>2825)
                     /locus_tag="VHV99_12685"
     CDS             complement(2505..>2825)
                     /locus_tag="VHV99_12685"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX3636326.1"
                     /translation="LMKRLNGEMFWSHVSGTTVDRRAPYARAIWTFLDLSVEPQMVSA
                     IRASLTPREREIAMLLLEGCSSKEIARRLEISPRTVHVHRASLLRKYSVSNTADLCTN
                     LLTL"
BASE COUNT          528 a          904 c          833 g          560 t
ORIGIN      
        1 cagcaggcgg cggggtgcta ttgactgacg gctcagacgc gttcttcaag gccgtggaag
       61 caaagatgtc tgccccagat ctccctctgt tcgccaaggg tcttctttgc aactggctgg
      121 tttgcctcgc gatctggatg gccgcacgta cgacgaacga cggagcgaag ctcgggctga
      181 tcttctggcc tattttcgca ttcgttgcaa gcggcttcga gcacagcgtc gcaaacatgt
      241 ttgcgtttgc gcttgcattg atggctgatc atcccgcctc aatcacgctt gcaggggcta
      301 tccataacga gttcttcgtg accgcgggga atctggccgg tggtgctatc ttcatggccc
      361 ttggctactg gctgcaggac ggcagtgtcg gccatgcatc tcaggcccgc gtgccggcat
      421 cggagcgggc tcgaaccaat tgaccgggca acgcctgccg catcattgat gacggatgct
      481 tttgtgcggt ggcggcgctt gcgccccgcc ccgctcaaac cccaatccgg aacagtaccg
      541 acttctcctg cgtctattca tccagccggt ttgcgccgaa ttcgcgccca agcccggatc
      601 gcttatctcc gccgaacggc gcgtccatca atgaatgcag gccgccgttg atcaccacgc
      661 ctggttcgtc gaggctgaca aaatcaagcg catggtgacg ctgcggaagg agacgaccgc
      721 tagcggtagc cccgcatatc gatgccgacg acatgagtcg ccggttgcgc atgttcgacg
      781 gcgccgcgtc acgcagcgag gacgcgttcc ggcggcgcca tgaactccgg ccccacggga
      841 tccttgacga actgcgcgac aattgcgtcg atctgcttga acgccttctc gtcgaggcgc
      901 cagccgaaca catcttcaat cccgtccagc tgtgccggac gacgtgcgcc ccacagcgcg
      961 atcgtcgggc cgcgatcgag cacccagcgc accgccagcg cgagcaccga cttgtcgaag
     1021 ttatcgcgcg cgaacacgtt gagtgctttc acggccgcga catactgctc gcgacgcggc
     1081 tcgcaaaact tcgggtcgcg ctgacgcagg tcgtcaccct cgaagcgtgt ctcgacgccg
     1141 atgcgccccg ccagcagccc gcggcacaac gcaccgtagg caagcgcgac gagcccatgc
     1201 tgttttgcgt acggcagcac gtcacggtcg atatcgcgtt cgaacaggtt gtacggcggc
     1261 tgcgtggccg acagcggcgc gacgctccgg aacgcatcca tttgcgcggg ggagaagttg
     1321 ctgacaccca gcgcccgcac cttgccttcc ttgcgcagat cgtcgagcac cgcggcggtc
     1381 tcttcgatcg gcaccagcgg atcgggccag tggacctgat acaggtcgat gtaatctgtg
     1441 cgcaagcgcc gcagcgtatc ctcgacttcc cggcgaatgc gctccggcga cgaattgcga
     1501 aacaccgcgc cgtcgtgcca ttccaatgcg accttggtcg cgatgactgc cttgtgccga
     1561 tacccatcgg ccagcgcctt gccgacgatc tcctccgaag cgccgaagcc gtacaccggc
     1621 gctgtatcga tcagattgac accgctatcg agcgcccggc ggatcaccgc gatcgagtct
     1681 ttctcgtcgg tgccgcccca catccatccg ccgatagccc aggtgcccag gccgatacgt
     1741 gagacggggg tcgttaaacc ttcaattgcg attgtttcaa tagtcatgtg aagctcttta
     1801 atcaaagtac tgcgtaaagc accgcgtccg ggtcgaatgc gatctgacca acggcgacgt
     1861 gcccctcgga cgggcaggcg atccgattat ttcagcggca tccggcgatc acaagaaggc
     1921 acctgacggt ttcatacttt ccccatggaa actaattgct acgacggcgc ttgcgcgcac
     1981 gacaacccgc aaaaaaaccg acgacctggc gcggaacatc atcgggcaga tggcggacag
     2041 gaccagtggg cgatccttgt catcgatgcg catgaaccac gtccagcttt ttcgtcctgc
     2101 tttacgtggc gctctcactg cctgtgatcg gcttcggcat tgcggtgcaa ctcgccgaca
     2161 ttcgacgcgc gacgctgctc ttcgtcggct tgacgaccgt tcttgtgctt atcgcgctgc
     2221 tcctgctgat gcgcgggcaa gcgaagcggg agccagaacg ccaaagcgat cggtgactga
     2281 aaagtgcggc attgctcgac gcatatcgct ggcattacat cgtgtcgggc gtcacgcagc
     2341 cgcggttttc gaaactgctg tacagaatga tcacgcccgc gcaagcggag catatcggcg
     2401 acgcgttggc gccgatagtg cagcggccgt ccgatatcga cgcgtaggtg ccgggctgat
     2461 ctgaccgtgg tggtcgaggc atctcttcgg ttcgcggcga agcttcaaag cgtcaacagg
     2521 ttcgtgcaca ggtcggccgt gttgctgacc gagtatttgc gcagcaagct cgcacgatga
     2581 acatgcacag ttcgtggact gatctccagc cgtcgtgcga tttccttgct gctgcaaccc
     2641 tcgagcagca gcatggcgat ttcccgctcg cgcggcgtga gcgaagcgcg tatggccgac
     2701 accatttgcg gttcaaccga caaatctaaa aatgtccaga ttgcgcgggc atacggggcg
     2761 cgcctgtcga cggtcgtccc gcttacatgg ctccagaaca tctctccatt cagccgcttc
     2821 atcag
//
LOCUS       DATASH010000458         4153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3433635,
            whole genome shotgun sequence.
ACCESSION   DATASH010000458 DATASH010000000
VERSION     DATASH010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3433635"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="VHV99_12690"
     CDS             <1..742
                     /locus_tag="VHV99_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006024571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEX3636327.1"
                     /translation="AWIKVNEVAKGRDGLQSPIVKNLHDEAVKAIIERTGAQDGDIIF
                     FAADRAKVVNDSLGALRLKIGHSEFGKANGLVETGWKPLWVIDFPMFEYDEEDNRYVA
                     AHHPFTSPKDEHLEYLETDPARCLAKAYDMVLNGWEIGGGSVRIHREEVQSKVFRALK
                     INAEEAQAKFGFLLDALQYGAPPHGGIAFGLDRIITMMAGADSIRDVIAFPKTQRAQC
                     LLTQAPSEVDERQLRELHIRLRQPEPKA"
     gene            944..1447
                     /gene="nudB"
                     /locus_tag="VHV99_12695"
     CDS             944..1447
                     /gene="nudB"
                     /locus_tag="VHV99_12695"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HEX3636328.1"
                     /translation="MPKPPKIPQSVLVVIHTPALDVLLIERADHAGFWQSVTGSKDLL
                     DEPFMETAAREVVEETGIVVGSARVPHSALFDWQYSIEYEIYPQFRHRYAEGVIHNTE
                     HWFSLEVPEQLEVTLAPREHTAFLWLPYEEAASRCFSSSNADAILQLPKRLAGRPDGG
                     QSGERGR"
     gene            1444..2703
                     /gene="clsB"
                     /locus_tag="VHV99_12700"
     CDS             1444..2703
                     /gene="clsB"
                     /locus_tag="VHV99_12700"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013338020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HEX3636329.1"
                     /translation="MSVGGARRFARLRQSLLGRRYWQRYRFTSGNDVKLLRSGDEFFA
                     ALIERIDAAQSDVVLETYIFCNDSAGQAVGAALMRAASRGVKVRVITDGIGTARLQMF
                     DEWPLAGIDHRIYNPHLFGRFGFSRTHRKIAVVDDQFAYCGGINIVDDYENNGEKLPY
                     RRWDFAVELHGPVVADVRQAFEVQWRRIRLGHRPPEALEPDLGPKVIASLGTLRRRRR
                     NRNEALWAGGQPCVAFVARDNLINRRAIEKAYLTAIGQARSEVLLANPYFMPGRKLRR
                     ALVFAARRGVEVKLIIGRKEFKALDYAVPFLYRALLKAGVQIAEYEKTLLHGKVAVVD
                     SNWGTVGSSNLDALSLMLNNEANVVLLNDPSIDALREAILVAFKDSRRIDEARYDARP
                     AGERALNWLAYTTYRTVMKLLTVGGYD"
     gene            2930..3529
                     /locus_tag="VHV99_12705"
     CDS             2930..3529
                     /locus_tag="VHV99_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX3636330.1"
                     /translation="MRKGEQTRAAILDAALDLASRDGLEGLTIGLLAERMQMSKSGVF
                     AHFGSREDLQVEVVREYHRRFEDEVFFPSLREPRGLPRLRAMISRWIEKRIQEVTTGC
                     IYISGAVEYDDRADNPVREQLVSSVTIWRAALTRAISQAMEEGHLRAETDPQLMLFEL
                     YSFTLGLHHDARFLHLPDAVRLTWAALEKLIISYQSESR"
     gene            3629..>4153
                     /locus_tag="VHV99_12710"
     CDS             3629..>4153
                     /locus_tag="VHV99_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase N-terminal
                     domain-containing protein"
                     /protein_id="HEX3636331.1"
                     /translation="MGQYAAPLRDMQFVLHELLNVEAEIKQMPKHADLDADTINAVLE
                     EAGKFCSEVLFPLNHSGDQEGCTYVGDGVVTTPKGFKEAYKQYVEAGWPALGCDPDYG
                     GQGLPAFVNNALYEMLNSANQAWTMYPGLSHGAYECLHAHGTPELQQRYLPKLVAGVW
                     TGTMCLTEPHCGTDL"
BASE COUNT          831 a         1259 c         1328 g          735 t
ORIGIN      
        1 ggcatggatc aaggtcaacg aagtggcgaa gggccgtgac ggtctgcaaa gcccgatcgt
       61 caagaacctg cacgacgaag cggtcaaggc gatcatcgag cgcaccggcg cgcaagacgg
      121 cgacatcatt ttcttcgcgg cagatcgcgc gaaggtggtg aacgacagcc tgggcgcact
      181 gcgtctgaag atcggtcatt cggaattcgg caaggccaac ggtctggtcg aaaccggctg
      241 gaagccgctg tgggtgatcg acttcccgat gttcgaatac gacgaggaag acaaccgcta
      301 cgttgccgcg catcacccgt tcacgagccc gaaggacgag catctcgagt atctggaaac
      361 ggatccggcg cgctgcctcg cgaaggccta cgacatggtg ctgaacggtt gggaaatcgg
      421 cggcggttcg gtgcgtattc accgtgaaga agtgcagagc aaggtgttcc gcgcgctgaa
      481 gatcaacgcg gaagaagcgc aagccaagtt cggcttcctg ctcgacgcgc tgcaatacgg
      541 cgcgccgccg cacggcggta tcgccttcgg tctggaccgc atcatcacga tgatggccgg
      601 cgcagattcg atccgcgacg tgatcgcatt cccgaagaca caacgcgcgc agtgtctgct
      661 cacgcaggcg ccgagcgaag tggacgagcg ccagctgcgc gagttgcaca tccgcctgcg
      721 gcagccggaa ccgaaggcct aaggtaccaa cctacggtgc cggccgggta agaagggtcg
      781 agcaggtcaa agctgccgga acatcggcag tttcaagcaa acgaaaaagg cgcgatgcgc
      841 ctttttcgtt ttttgcgtta cagtcgcagc aaccggtctc gaccgctcat tggaccactc
      901 gccgacatgt agcgcgccaa gcgttgcgcc cagttttcaa gccatgccga aaccgccgaa
      961 gatcccgcaa tccgtactcg tcgtcatcca tacgcccgcg ctggacgtgc tgttgatcga
     1021 gcgtgccgac catgcaggtt tctggcagtc ggtgacgggc tcgaaagacc tgctcgacga
     1081 acccttcatg gagacggccg cacgcgaggt ggtcgaggaa accggcatcg tggtcggcag
     1141 tgcgcgggtg ccgcacagcg cgctgttcga ttggcagtac tcgatcgaat atgagatcta
     1201 cccgcagttc cggcatcgct atgccgaagg cgtgatccac aacaccgagc attggttcag
     1261 cctggaagtg cccgaacagc tcgaagtgac gctcgcgccg cgcgaacaca ccgcgttcct
     1321 gtggttgccg tatgaagagg cggcgtcgcg ctgcttttca tcgtcgaatg cggatgcgat
     1381 cctgcaattg cccaagcggc tcgctgggcg cccggatggc gggcagtccg gggagcgtgg
     1441 ccggtgagcg tcggtggcgc gcgccgtttc gcgcgactcc ggcaatcgct gctcggccgc
     1501 cgctactggc agcgctaccg cttcaccagc ggcaacgacg tcaagctgct gcgttccggc
     1561 gacgagttct tcgccgcgct catcgaacgc atcgacgcgg cgcagagcga cgtggttctc
     1621 gaaacctata tcttctgtaa cgatagtgcc ggccaggccg tcggcgccgc gctgatgcgc
     1681 gccgcgtcgc gcggcgtgaa ggtgcgcgtg atcaccgacg gcatcggcac ggctcgcctc
     1741 cagatgttcg acgaatggcc gcttgcgggc atcgatcatc gcatctacaa cccgcacctg
     1801 ttcggccgct tcggcttttc gcggacgcac cgcaagatcg cggtggtgga cgatcagttc
     1861 gcgtattgcg gcggtatcaa catcgtcgac gattacgaga acaacggcga gaagctgccg
     1921 taccggcgct gggatttcgc ggtggagctg catgggccgg tggtcgccga tgtccgccag
     1981 gcgttcgagg tgcaatggcg gcgcatccgc ctcgggcatc ggccgcccga ggccctcgag
     2041 cccgatctcg ggccgaaggt catcgcgtcg ctcggcacct tgcgccgccg gcgccgcaac
     2101 cgcaacgagg cgctgtgggc tggcgggcag ccgtgcgtgg ctttcgtcgc acgcgacaac
     2161 ctgatcaacc gccgcgccat cgaaaaagcg tatctgaccg cgatcggcca ggcccgcagc
     2221 gaagtgctgc tggccaaccc atacttcatg ccgggccgca agctgcgacg ggcgctggtg
     2281 ttcgcggcgc ggcgcggtgt cgaggtgaag ctgatcatcg ggcgcaagga attcaaggcg
     2341 ctcgattacg cggtgccgtt tctgtatcgc gcccttctca aagccggcgt gcagatcgcc
     2401 gagtacgaga aaaccctgct ccacggcaag gtcgcggtgg tggattcgaa ctgggggacc
     2461 gtcggctcgt cgaacctcga tgcgttgagc ctgatgctca acaacgaagc caacgtcgtg
     2521 ctgctgaacg acccgtcgat cgacgcgctg cgtgaagcga tcctggtcgc attcaaggat
     2581 tcgcgccgga tcgatgaggc gcgctacgac gcccgtccgg cgggcgagcg cgccctcaac
     2641 tggctcgcct acacgaccta ccggaccgtg atgaagctgt tgacggtggg tggctatgac
     2701 tagcgctcgc cccactctca cataaacgca aaggtgtggc gtccatgaaa ggaagcaaaa
     2761 gaaggcaaac gcctctaaaa aatctccttc ctctgattga cggatgcagc agaattgcaa
     2821 ccgggttaga aaccggtttc taataaagtc cttgtttcgc ggacggcggc actcaataat
     2881 agtacggccg ttcgattttc tttcggtcac attagaacgg taaaaaccta tgcgaaaagg
     2941 cgaacaaaca cgagccgcga ttctcgacgc agcactcgat ctggcaagcc gcgacggact
     3001 ggaaggtctg acgattgggc tgctggccga acgcatgcaa atgagcaaaa gcggtgtgtt
     3061 cgcgcatttc gggtcgcgcg aggatctgca ggtcgaggtc gtgcgcgaat accaccgtcg
     3121 ttttgaagac gaggtgtttt tcccgagctt gcgcgagcct cgtggattgc cgcgcttgcg
     3181 cgcgatgatt tcccgctgga tcgagaaacg cattcaggaa gtcaccactg ggtgcatcta
     3241 catcagcggc gccgtcgagt acgacgaccg tgcggacaac ccggtgcgtg agcaactggt
     3301 gtcgagcgtg acgatctggc gcgcggcgct cacccgggcg atttcgcagg caatggaaga
     3361 agggcatctg cgtgccgaga ccgatccgca gctgatgctg ttcgaactgt acagcttcac
     3421 gctcggcctg catcacgacg cgcgcttcct gcacttgccg gatgcagtgc gtctgacgtg
     3481 ggccgcgctg gaaaaactga ttatttcgta tcagagcgaa agccgttagc agggccgcct
     3541 aacctgaaca gattgagcgg cgccgatcga tccgatccac ggactcggct aacagcctgg
     3601 ctccttgtca aacttgggag atagagtcat gggacagtac gccgcgccgc tgcgcgacat
     3661 gcaattcgtg ttgcacgagc tgcttaacgt cgaagccgaa atcaagcaga tgccgaagca
     3721 cgcggatctc gacgcggaca cgatcaacgc cgtgctcgaa gaggctggca agttctgctc
     3781 cgaagtgctg ttcccgctca atcacagcgg tgaccaggaa ggctgcacgt acgttggcga
     3841 cggcgtcgtg accacgccga agggcttcaa ggaagcctat aagcagtacg tggaagccgg
     3901 ctggccggca ctcggctgcg acccggacta cggcggccag ggcctgcccg cgttcgtcaa
     3961 caacgcgctg tatgaaatgc tgaactcggc gaaccaggcg tggacgatgt acccgggcct
     4021 gtcgcacggc gcgtatgaat gcctgcacgc gcacggcacg ccggagctgc aacagcgtta
     4081 tctgccgaag ctggtcgcgg gcgtctggac cggcacgatg tgcctgaccg aaccgcattg
     4141 cggcaccgac ctc
//
LOCUS       DATASH010000459         1751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_664507,
            whole genome shotgun sequence.
ACCESSION   DATASH010000459 DATASH010000000
VERSION     DATASH010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664507"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..1043
                     /locus_tag="VHV99_12715"
     CDS             138..1043
                     /locus_tag="VHV99_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX3636332.1"
                     /translation="MRKRFDRTILAGLAGAWVALAVSGCANVGQPNAQNGVVAGAAAA
                     SGVPVANAAGTASGDPAAAAPLIKSPPLVYRVRRGDTLARVAQHHHISVKQLQAWNGL
                     KASARLKPGQVLHVASPETVRAVKQANAAAAAQPAAQTSSQATAQTSPQASAQSAAPA
                     PDPTEVREVTQETRRHASSVTLAWPAEGGVVEAFQPGETRGIEIGGKAGDPVRAAADG
                     KVMYAGTGLNSYGSLIIVQHNKDFLTAYSHNRKLLVKMGDIVRKGQQIAEMGDESNSR
                     VSVGFELRRDGKPVDPLPYLPQGRG"
     gene            complement(1302..1700)
                     /locus_tag="VHV99_12720"
     CDS             complement(1302..1700)
                     /locus_tag="VHV99_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636333.1"
                     /translation="MNDPQCTQFLTDIRRPLLALHKAILDHERAAYEKEFGPVTPAAF
                     LQVLINGSGFRWLTPLSTVIASVDEVLDDAEADAGDRLAIAEAVVGLFSSDQPNNSFL
                     PRYLPILQADPAILHYHGEVSRLLRGAQTM"
BASE COUNT          343 a          523 c          580 g          305 t
ORIGIN      
        1 gccgacttca cgcggaatgc ggctcgtaac gtctgtatca gagtgtgtgg ggcggggcgt
       61 ttttattaaa tgtgacttta caattccgcc agatttttga tcggcaagca gcgcatagac
      121 agcaggagtc gaacagtatg agaaagcgtt tcgacaggac gatattggcc ggtctcgccg
      181 gcgcatgggt tgcgctggcc gtcagcggtt gcgcgaacgt cggccagccg aatgcgcaga
      241 acggcgtggt tgccggcgcc gcagcggcgt ccggcgtgcc ggtggcgaat gccgcgggaa
      301 ccgcgtctgg cgacccggca gcagcggcgc cgctgataaa gtcgccgccg ctcgtgtatc
      361 gcgtaaggcg tggcgacacc ctcgcgcggg tcgcgcagca tcatcacatc agcgtcaagc
      421 agttgcaggc gtggaatggg ttgaaggcgt cggcgcggct gaagccgggg caggtgctac
      481 atgtcgcgtc gccggagacg gtgcgcgcgg tgaagcaggc caatgcggcg gccgcggcgc
      541 aacccgcggc gcagacttcc tcacaagcta cagcgcaaac gtccccgcag gcttcggctc
      601 aatccgccgc accggctccg gatcccaccg aagtccgcga agtcacccag gaaacccgcc
      661 ggcatgcgag tagcgtgacg ctcgcgtggc ctgccgaagg cggcgtcgtc gaggcgttcc
      721 agccgggcga aacgcgcggc attgaaattg gcggcaaagc gggcgatccg gtgcgagccg
      781 ccgccgacgg caaggtgatg tacgcgggca ccggactgaa cagctatggc agtttgatca
      841 tcgtgcagca caacaaggat tttctgactg cgtattcgca taaccgtaag ctgctggtca
      901 agatgggcga catcgttcgc aagggacagc agatcgcgga gatgggcgac gaaagcaact
      961 cgcgtgtgtc ggtcggcttc gaactgcggc gcgacggcaa gccggtcgat ccgctgcctt
     1021 atctgccgca ggggcgcggc tgagcttcgg ccttcccgca aaagctcacc ttgaatttat
     1081 tcagcaaaac caggtaattt catcgaccgc gttgctcgac cgcacattct gcggtcgtcg
     1141 aatcgttgcg aatgtaccgt cacgcgccgg tattcgggtc aatcgcgacg aagtagctgc
     1201 aaataactcc gcggcatcga cccgaaaccg ttgttgcaac ggtgaggaaa tacaggagcc
     1261 ggcaggcagc gcacacccgc cgtgctccgg ctgaatcgcc gctacatcgt ttgcgccccg
     1321 cgcagcaatc gcgatacctc gccgtggtaa tgcaggatcg cgggatcagc ctgcaatatc
     1381 ggcagataac gcggcaggaa actgttgttc ggctgatcgg acgagaacag gccgaccacg
     1441 gcttcggcga tcgcgagacg gtcgccggca tcggcttccg cgtcgtcgag aacttcgtcg
     1501 acgctcgcaa tcaccgtcga tagcggcgtc agccagcgaa accccgaacc gttgatcagc
     1561 acctgcagga acgccgcggg cgtcaccggg ccgaactcct tttcgtacgc ggcgcgttcg
     1621 tgatcgagaa tcgccttgtg cagcgcaagc aacggtcggc gaatgtccgt gaggaattga
     1681 gtgcattgcg ggtcgttcat cggcggctcc tgcggctggg gtgactcgac ggattgatcc
     1741 gtcgatggta a
//
LOCUS       DATASH010000460         5739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4226811,
            whole genome shotgun sequence.
ACCESSION   DATASH010000460 DATASH010000000
VERSION     DATASH010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5739
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4226811"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..646)
                     /gene="orn"
                     /locus_tag="VHV99_12725"
     CDS             complement(35..646)
                     /gene="orn"
                     /locus_tag="VHV99_12725"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEX3636334.1"
                     /translation="MTDILASTDQPPLVRSDMNLVWLDMEMTGLEPDTDRIIEIAVVV
                     TNSTLDRMVEGPVLAIHQSDETLAKMDQWNQNTHGRSGLIDRVKASTVSEADATEQIR
                     EFLAQYVPPGKSPMCGNSICQDRRFMARWMPDLERFFHYRNLDVSTLKELCRRWQPVI
                     YKGFQKRAMHTALADIHESIDELKYYREHFLIPSAPEAGASAE"
     gene            856..2115
                     /locus_tag="VHV99_12730"
     CDS             856..2115
                     /locus_tag="VHV99_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX3636335.1"
                     /translation="MPTLYFTVLFVVAVVAMVGTKLWLASRQIRFVAGHREQVPSQFA
                     GTIALSAHQRAADYTVERTRLTMIEIIVGAAVLIGLTLLGGVQALDLAISDWLGRGYA
                     GQIALVAAVIAITSVIDLPFEYYRQFVVEQRFGFNRMSKGIFFLDRLKGVLLGAAFGL
                     PLLFVVLWLMNQAGSLWWLWTWIVWVAFQMLVLVLYPSFIAPLFNKFEPLKDEALKSR
                     IEALMKRCGFAAKGLFVMDGSRRSAHGNAYFTGFGAAKRIVFFDTLLARLSGNEIEAV
                     LAHELGHFKRRHVIKRMVVMFAISLVMLALLGWLTQCVWFYEGLGVRPSLIGGNSGLA
                     LVLFFLALPVFLFFVTPLGSLSSRKHEFEADAFAATQTDAQDLVNALVKLYEDNASTL
                     TPDPLYTAFYYSHPPASQRIDRLLRHA"
     gene            2112..3050
                     /gene="rsgA"
                     /locus_tag="VHV99_12735"
     CDS             2112..3050
                     /gene="rsgA"
                     /locus_tag="VHV99_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEX3636336.1"
                     /translation="MSRSPKAPRAPSGTRVGGRVIAAHGRHYLVAPEDGSPMLQCFPR
                     GKRSEVAVGDRVIYEPTSVDQGVIVEIGERRNLLYRSDQYKSKLFAANLDQLLIVLAT
                     EPHFSEDLLGRALVAAEANGLKPLIVLNKIDVTGELEGARRRLESYRALGYTVVEVSI
                     TTQPDAARAALTEHLQGHSTLLLGQSGMGKSTLVNLLIPNAEVATREISTALNSGRHT
                     TTFTRLYPLPGNDGGALIDSPGFQEFGLHHLTEGRLERAFPEFRPLLPNCRFYNCHHL
                     QEPGCAILEAVADGHVRRERHALYAQLVHEASQIVR"
     gene            3081..3401
                     /locus_tag="VHV99_12740"
     CDS             3081..3401
                     /locus_tag="VHV99_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006052833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEX3636337.1"
                     /translation="MKLMRAPNLIIGQHWVNVLATAGIACELHNRYLNGALGDIPADQ
                     CAPELWLVDERDEMMARRLIDAASQGPAAGSPSWQCRQCGETLEAQFTVCWQCGTVRD
                     PLDG"
     gene            complement(3620..4558)
                     /locus_tag="VHV99_12745"
     CDS             complement(3620..4558)
                     /locus_tag="VHV99_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012213825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CobD/CbiB family protein"
                     /protein_id="HEX3636338.1"
                     /translation="MTFFSVLLALIIEQVRALSLNNPVAALLQYHAESAAHGFDAGKP
                     KHGLLAWLVVVVPWTLAVALVYYVLYHIHFALAFLWNVAVLYFTLGFRQFSHYFTDIH
                     LALNNDDVPRAREILNEWTGLDTVDMPVSEIVRHTLIHAVVASHRHVFGVFFWFLIPV
                     GPAGAVLYRIAEYLARAWARPVDDRTAAFSSFAQRAFFVIDWVPARLTSLGFAIVGNF
                     EDAIYAWRNHARQWPDANDGVLLAAGSGALGARLSGPLAEPSSLDALATGDGGPMQVG
                     DDCTPRTLQSAVGLVWRAVVLWMILLLMLTIAVWLA"
     gene            complement(4681..5397)
                     /locus_tag="VHV99_12750"
     CDS             complement(4681..5397)
                     /locus_tag="VHV99_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEX3636339.1"
                     /translation="MIRPVFEPETLPVEATGADLPPVAAGRLTPDGLRGRFEQRLSWT
                     PEPIVEAPWRDTHVDPRVAAVLVPLVLREQGLTVLLTQRADHLNDHAGQVSFPGGRHE
                     PFDADATATALREAQEEVGLDPSRVEILGALPDYLTGTGFRVTPVIGLVHPPFTLKAD
                     ALEVAEVFEVPLAFLMNPAHHEERVFRYEGGERRFFAMPYPRAASVQVEGRGSQPGGH
                     YFIWGATAAMLRNFYRFLAA"
     gene            complement(5490..>5739)
                     /gene="rplS"
                     /locus_tag="VHV99_12755"
     CDS             complement(5490..>5739)
                     /gene="rplS"
                     /locus_tag="VHV99_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEX3636340.1"
                     /translation="YEGVVIAKRNRGLNSSFIVRKISSGEGVERTFQTYSPLLASIVV
                     KRRGDVRRAKLYYLRDRSGKSARIKEKLVSKDRAASQA"
BASE COUNT         1072 a         1770 c         1814 g         1083 t
ORIGIN      
        1 gcccgggagt gaacacccag gcgtgcccgg gcgtttattc cgcgctcgcc cccgcttccg
       61 gcgccgacgg aatcaggaag tgctcgcggt agtacttcag ttcgtcgatc gactcgtgga
      121 tgtcggcgag cgccgtgtgc atcgcgcgct tctggaagcc cttgtagatg accggctgcc
      181 agcggcggca cagttccttc agcgtgctga cgtcgagatt gcggtaatgg aagaatcgct
      241 ccagatccgg catccagcgc gccatgaaac ggcggtcctg gcagatcgag ttgccgcaca
      301 tgggcgattt gccgggcggc acgtactgcg ccagaaactc gcgaatctgt tcggtggcgt
      361 cggcttcgct gaccgtcgaa gccttcacgc ggtcgatcag ccccgagcgg ccatgcgtgt
      421 tctgattcca ctgatccatt ttggccagcg tctcatcgct ctgatggatc gccagcaccg
      481 gcccttcgac catcctgtcg agcgtcgaat tcgtcaccac caccgcgatt tcgatgatgc
      541 ggtcggtgtc cggctcgagg ccggtcattt ccatgtccag ccagacgaga ttcatgtcgc
      601 tgcgcacgag cggcggctgg tcggtggagg cgaggatgtc agtcatgaag gcaaccctga
      661 tggccgcgca aaaggcttcg ctggaagcac gcttgccgcg gcgattgttc gtatgagatt
      721 ggtttgcgcc ggttcgagcc ggttcgagcc ggttcgagcc ggggtttggc cgcgtttcgg
      781 ccgggtttcg gcttaccccc gccgcgaggc gggaagaaac atataattct cgcatagata
      841 ccacggaatc cccggatgcc taccctgtac ttcactgttc tgttcgtcgt cgccgtcgtg
      901 gcgatggtcg gcaccaaact gtggctcgcg tcgcggcaga tccgcttcgt cgccggccat
      961 cgcgagcagg tgccgagtca gtttgccggc accatagcgc tcagcgcgca ccagcgcgcc
     1021 gcggactaca ccgtcgaacg cacgcgtcta acgatgatcg agatcatagt cggcgccgcc
     1081 gtgctgatcg ggctgacgct gctgggcggc gtgcaggcgc tcgatctggc gatctccgac
     1141 tggctcggcc gcggctacgc cggccagatc gcgctggtcg cggcggtgat cgcgattacc
     1201 agcgtgatcg acctgccgtt cgagtactac cgtcagttcg tggtcgagca acgcttcggc
     1261 ttcaaccgga tgagcaaggg catcttcttc ctcgaccgtc tcaagggcgt gctgctcggc
     1321 gccgcattcg gcctgccgct gctgttcgtc gtgctgtggc tgatgaacca ggccggcagc
     1381 ttgtggtggc tgtggacctg gatcgtctgg gtcgcgttcc agatgctcgt gctggtgctg
     1441 tatccgtcgt ttatcgcgcc gctcttcaac aagttcgagc cgctcaagga cgaagcgttg
     1501 aaaagccgca tcgaagcgct gatgaagcgc tgcgggttcg ccgccaaggg cctgttcgtg
     1561 atggacggca gccgccgttc ggcgcacggc aacgcgtatt tcaccggctt cggcgcggcc
     1621 aagcggatcg tgttcttcga cacgctgctc gcgcgcctgt cgggcaacga gatcgaagcg
     1681 gtgctcgcgc acgaactcgg tcacttcaag cgtcgtcacg tgatcaagcg gatggtcgtc
     1741 atgttcgcga tcagcctcgt gatgcttgcc ctgctcggat ggctgacgca atgcgtgtgg
     1801 ttctatgaag gactaggcgt gcgcccgtcg ctgatcggcg gcaatagcgg cttggcgctg
     1861 gtgctgttct tcctcgcgct gccggtgttc ctgttcttcg tgacgccgct cggcagcctc
     1921 agttcgcgca aacacgagtt cgaagccgat gcgttcgccg ccacgcaaac cgacgcgcaa
     1981 gacctggtca atgcgctcgt caagctgtat gaggacaacg cgtcgacact gacgcccgat
     2041 ccgctctaca ccgcgttcta ctattcgcat ccgccggcgt cgcagcggat cgaccgcctg
     2101 ctgcggcacg catgagccgc tccccgaaag cgccgcgcgc gccgtccggc acgcgcgtag
     2161 gcggccgtgt gattgccgct catgggcggc attacctggt cgcaccggaa gacggcagcc
     2221 cgatgctcca gtgcttcccg cgcggcaagc gcagcgaagt ggccgtgggc gatcgcgtga
     2281 tttacgagcc gacttcggtg gatcagggcg tgatcgtcga gattggcgaa cgacgcaatc
     2341 tgctatatcg ctcggatcag tacaagtcga agctgtttgc cgccaatctc gatcagttgc
     2401 tgatcgtgct ggccacggaa ccgcacttca gtgaagacct gcttggccgc gcgctggtcg
     2461 ccgccgaggc gaacgggttg aagccgctga tcgtgctgaa caagatcgac gtgacgggcg
     2521 agctggaagg cgcgcgcagg cggctcgaat cgtatcgcgc gctgggttat accgtggtcg
     2581 aagtctcgat tacgacgcag ccggacgccg cacgtgccgc gttgaccgaa catctgcaag
     2641 gtcactcgac gctcttgctc ggccagtcgg gcatgggcaa atcgacgttg gtgaatctgc
     2701 tgattccgaa tgccgaagtc gccacgcgcg aaatctcgac cgcgttgaac agcggccggc
     2761 ataccacgac cttcacgcgt ctctaccctc tgcccggaaa cgacggcggt gcgttgatcg
     2821 attcgccggg cttccaggaa ttcggtctgc atcatctgac cgaaggccgg ctcgaacgcg
     2881 cgtttcccga gttccggccg ttgctgccga attgccgctt ctacaactgc catcacttgc
     2941 aggagccggg ctgcgcgatt ctcgaagcgg tcgcggatgg acatgtccgg cgcgaacgcc
     3001 atgccctgta tgcgcaactg gtgcacgaag ccagccagat cgtgcgctga cgcgcgccgc
     3061 tgtcaaacta tggattcacg atgaaattga tgcgcgcgcc gaatctgatc atcggacagc
     3121 attgggtcaa tgtgctggcg acggcgggga tcgcttgcga gttgcacaac cggtatctga
     3181 acggcgcgct tggggatatt ccggcggatc agtgcgcgcc tgagttgtgg cttgtcgatg
     3241 agcgggatga aatgatggcg cggcgcctga tcgatgcggc ttcgcagggt cccgccgcag
     3301 gttcgccttc gtggcaatgc cgccagtgcg gagagacgct tgaagcgcag ttcacagtgt
     3361 gctggcagtg tgggacagtg cgcgatccgt tggatgggtg acgtgcctgg tggcgtcgtg
     3421 ggtgggcagt tgctggagtg agccggaagt gagtggcgtg gttgccgtgg ttgccgtggt
     3481 tgccgtggtt gccgtggcct cgttgagcaa ggatgaagcg cttcgggtgc aggcaacgtg
     3541 aacggtacga ccgttgcgcc gcaggcaata aaaaaaccgg acatttgtcc ggcttcttct
     3601 gcactgcatt agcatcgctt caagcgagcc agactgcaat cgtcagcatc aacaacaaaa
     3661 tcatccacag caccaccgcg cgccatacca gacccactgc cgattgcagc gtgcgcggcg
     3721 tgcagtcgtc accgacctgc attggaccgc cgtcgccggt agccagcgcg tcgaggctcg
     3781 acggctcggc aagcggccca ctcagacgcg cacccagcgc cccactgccc gcggccagca
     3841 acacgccgtc gttcgcatcc ggccactgac gcgcgtggtt gcgccacgca taaatcgcgt
     3901 cttcgaaatt accgacgatc gcaaagccaa gcgacgtaag ccgtgccggc acccagtcga
     3961 tcacgaagaa agcacgctgc gcgaagctcg aaaacgccgc cgtgcgatcg tcgaccgggc
     4021 gagcccacgc gcgcgccaga tactcggcaa tccgatacag caccgcacca gcgggcccga
     4081 ccggaatcag gaaccagaaa aacacaccga acacgtgccg gtgcgaggcg accaccgcat
     4141 gaatcagcgt atgacgcacg atctcgctga ccggcatgtc gacggtgtcg agtccggtcc
     4201 attcattcag gatttcacgt gcgcgcggca catcgtcgtt gttcaacgcc agatgaatgt
     4261 cggtgaagta gtgactgaac tggcggaagc cgagcgtgaa gtacagcacc gcgacgttcc
     4321 acaggaaggc cagcgcaaag tgaatgtgat agagcacgta gtagacgagc gcgaccgcca
     4381 gcgtccacgg caccaccacc accagccacg cgagcaggcc atgcttgggc ttgccggcat
     4441 cgaacccgtg cgccgccgac tccgcatggt attgaagcaa tgccgccacc ggattgttca
     4501 gcgacagcgc gcggacctgt tcaataatca gagccagcaa tacggagaaa aaagtcatgc
     4561 ggtgcgccgt gcttttcgag ttttacgatt gttttataac gatagcacag ggtcggatgc
     4621 gactgagcgc agacgttgac aaacacccgg caactgcaca gtccgatatg tcgcgctggc
     4681 tcacgccgct aaaaaacgat agaagttgcg cagcatcgcg gccgttgcgc cccagatgaa
     4741 atagtgaccg cctggctggc tcccgcgacc ttcaacttgc accgatgccg ctcgcggata
     4801 gggcatggcg aagaagcggc gctcgccgcc ttcgtaacga aatacgcgct cttcgtgatg
     4861 cgcggggttc atcagaaaag cgagcggcac ttcgaaaact tcggcgactt cgagtgcgtc
     4921 ggctttcaac gtgaatggcg gatgcacgag accgatcaca ggtgtcacgc gaaaaccggt
     4981 gcccgtcaga taatcgggca gcgcaccgag aatttcaacg cgcgacggat cgagaccgac
     5041 ttcttcctgc gcctcgcgca atgccgtggc agtcgcgtcc gcgtcgaacg gttcatgtcg
     5101 cccgccggga aagctcacct ggccggcgtg gtcgttcaga tggtcggcac gttgggtcag
     5161 cagcacggtg aggccttgct cacgcagcac cagtggcacc agcaccgcgg caacgcgcgg
     5221 gtcgacgtgc gtgtcacgcc aaggcgcttc gacaatcggt tctggcgtcc agcttaagcg
     5281 ctgctcgaaa cgcccacgca gaccatcggg cgtcagacgc cctgcggcca cggggggtag
     5341 atcggcgccg gtcgcttcga cgggcagggt ttcgggctca aagacagggc ggatcaacgg
     5401 gtctctcaga atgagcggat aaggacatgc cgctattttg acctggcaaa caaaaaagca
     5461 cccgcgaggg gtgctttttc ttacagcatt tacgcctggg aagcagcgcg gtctttcgag
     5521 accagctttt ccttgatccg agccgacttg cccgaacggt cacgcaggta gtacagcttg
     5581 gcacgacgca cgtcgccgcg acgcttcacg acgatgcttg ccagcagcgg cgagtacgtc
     5641 tggaacgtac gctcgacgcc ttcgcccgac gaaatcttgc ggacgatgaa cgacgaattc
     5701 agaccacggt tacgcttggc gatcacgacg ccttcgtaa
//
LOCUS       DATASH010000461         5336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6995573,
            whole genome shotgun sequence.
ACCESSION   DATASH010000461 DATASH010000000
VERSION     DATASH010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5336
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6995573"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1162
                     /locus_tag="VHV99_12760"
     CDS             <1..1162
                     /locus_tag="VHV99_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019254711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX3636341.1"
                     /translation="ETDKLASELIARHPEFKLQKIVVSEAGASVYSASELAAKEFPDM
                     DVSLRGAVSIARRLQDPLAELVKIEPKAIGVGQYQHDVNQRDLARSLDAVVEDCVNAV
                     GVDANTASVALLARVSGLNATLARNIVDYRDANGPFPSREHLRKVPRLGDKTFEQAAG
                     FLRINNGENPLDRSSVHPEAYPVVERMLAKISKHVGEVLGNRDALRGLSPAEFVDERF
                     GLPTVRDILLELEKPGRDPRPEFKTATFREGVEKVSDLTPGMVLEGVVTNVAAFGAFI
                     DIGVHQDGLVHVSAMSTKFIKDPHEVVKAGQIVKVKVLEVDVKRQRIALTMRLDDEFG
                     ASAAPRSGGGAQQDRGGFNRPGGGGRGGQQPRSREPEAGGAMAAAFAKLKQK"
     gene            complement(1402..3288)
                     /locus_tag="VHV99_12765"
     CDS             complement(1402..3288)
                     /locus_tag="VHV99_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HEX3636342.1"
                     /translation="MTHNNHVHKQPLPSLAVAAIGVVFGDIGTSPLYSLKEAFSPAHG
                     IPLTDQSILGVISLLFWAIVIVVGVKYVLFVMRADNNGEGGVLALMALALRSLDDKSK
                     MAGLLMMLGIFGACMFYGDAVITPAISVISAVEGLEIAAPHLSHLVLPLTMVILVLLF
                     WIQRHGTATVGRLFGPIMVLWFLVLAALGLWHILQSPNVIRALNPYYAYTFMGAHVLQ
                     AYVVLGSVVLVLTGAEALYADMGHFGAKPIRMAWYFLVMPSLVLNYFGQGALLMRDPK
                     AIENPFFLLAPDWALLPLVVLSTVATVIASQAVISGAYSLTSQAIQLGYVPRMKILHT
                     SELAIGQIYVPVVNWMLLFIILCIVIAFKSSDNLAAAYGIAVTATMVITTILACVVMV
                     NVWNWNKFLVGLIIGAFMAVDLGFFGANLLKVAEGGWLPLGIGALLFFLLMTWFKGRM
                     IVKERTAADGIPLMPFLQGLLAHPPHRVSGTAIYLTGSDSLVPVSLLHNLKHNKVLHE
                     RTIFLTFVTRDIPYVNDADRVTVKDFGGGLFLVKAVYGFNETPDVKAVLLEVGRTHDM
                     TFEMMDTSFFLARETVVPTQLPGMSVWRERVFAWMHQNAAKPTDFFSIPANRVVELGT
                     KIEI"
     gene            complement(3603..4193)
                     /locus_tag="VHV99_12770"
     CDS             complement(3603..4193)
                     /locus_tag="VHV99_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEX3636343.1"
                     /translation="MVQGVPMIAMKDPRNDDKNLWVGWDEYHRLIELLALAVHESGWK
                     FDKILCLARGGLRVGDQLSRICDLPLAILATSSYREAAGTEQGELDIAQYITMTRGEL
                     HGNVLLVDDLVDSGVTLARVQQHLKERYPAITAVRSAVLWYKGCSKVKPDYHVQYLPT
                     NPWIHQPFEEWDTVRPHNLGAWIKRGTQQEQESSGT"
     gene            complement(4197..>5336)
                     /locus_tag="VHV99_12775"
     CDS             complement(4197..>5336)
                     /locus_tag="VHV99_12775"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEX3636344.1"
                     /translation="MHPGVACYIGNGVVLSPEALFKEIGELEAAGVDVQNRLFISEAT
                     TLILPYHIAIDQGREARRGASKIGTTGRGIGPAYEDKVARRGLRVQDLFEPESFAERL
                     RENLDYHNFVLTQYLGVAAVDFQQTLDTMLSYADRLKPMVTDVSRRLYDENAAGNNLL
                     FEGAQGTLLDIDHGTYPFVTSSNCVAGAATAGAGVGPQKLNYILGITKAYCTRVGSGP
                     FPSELYDADNAARQEPIGLELAKVGKEFGSVTGRPRRTGWLDAAALRRSIQINGVSGL
                     CMTKLDVLDGLDEVKLCIGYTVDGKTVDLLPRGASEVARCVPVYETFAGWKESTVGIT
                     EWDKLPASARAYLTRVQEVAGIPIDMVSTGPDRDETILLRHPFKV"
BASE COUNT         1103 a         1678 c         1618 g          937 t
ORIGIN      
        1 tgaaacggac aagctggcga gcgaactgat cgcgcgtcat ccggagttca agctgcagaa
       61 gatcgtcgtc tcggaagccg gcgcatcagt gtactcggcc tccgaactcg cggcgaagga
      121 attcccggat atggacgtgt cgctgcgcgg cgccgtgtcg attgcgcgcc gtctgcagga
      181 tccgctcgcg gaactcgtga agatcgaacc caaggcgatc ggcgtcggcc aataccagca
      241 cgacgtgaat cagcgcgacc tggctcgctc gctcgacgcg gtggtcgagg attgtgtgaa
      301 cgcggtcggc gtggatgcga atacggcatc ggtcgctttg ctggcgcgcg tgtcaggtct
      361 gaacgcaacg ctggctcgca acattgtcga ctatcgcgat gcaaacggcc cgttcccgtc
      421 gcgcgaacat ctgcgcaagg tgccacgtct cggtgacaag acgttcgagc aggcggcggg
      481 cttcctgcgc atcaacaacg gcgagaatcc gctcgaccgc tcgtcggttc acccggaagc
      541 gtatccggtc gtggaacgca tgctcgcgaa gatcagcaag catgtcggcg aagtgctcgg
      601 caatcgcgac gcgctgcgcg gcctgtcgcc ggcggaattc gtcgacgaac gcttcggcct
      661 gccgaccgtg cgcgacattc tgctcgaact cgaaaaaccg ggtcgcgatc cgcgccccga
      721 gttcaagacc gcgaccttcc gcgaaggcgt cgagaaggtc agcgatctga cgcccggcat
      781 ggtgctcgaa ggcgtcgtga cgaacgtcgc ggcgttcggc gcgttcatcg acatcggtgt
      841 gcatcaggac ggtctggtgc acgtatcggc catgtccacg aagttcatca aagacccgca
      901 cgaagtcgtc aaggccggcc agatcgtcaa ggtgaaggtg ctggaagtcg acgtgaagcg
      961 tcagcgtatc gcgctgacca tgcgcctcga tgacgagttc ggcgcgtcag cagcgccgcg
     1021 cagcggcggt ggcgcccagc aggatcgcgg cgggttcaat cgcccgggcg gcggcggacg
     1081 aggcggccag caaccgcgct cgcgcgagcc ggaagcaggc ggtgcaatgg ccgccgcatt
     1141 tgccaagctg aaacagaaat aaacctgcgt gctcggtgcg tgattcactt cacgcgccgg
     1201 cgttcacgaa aaatcatccc gcagcgcgcc ggcgttcgtg cctccaacgg cctggcgact
     1261 ccgccaagtc ccagcatcac cggaatactt ggggcgtgcg cgtgcgagag agagcgagac
     1321 gcacccaaac aaaaagccca cgcaaaacgc gtgggctttt ttatcgcgtc tcagtgttga
     1381 agacgcattc aaccgtgctg ctcagatctc gatcttcgtt cccagctcca ccacgcgatt
     1441 cgctggaata ctgaagaagt cggtcggctt cgccgcgttc tgatgcatcc acgcgaacac
     1501 gcgttcacgc cacacggaca tgcctggcaa ctgcgtcggc accaccgttt cgcgcgcaag
     1561 gaagaacgag gtgtccatca tctcgaaggt catgtcatgc gtgcggccca cctcgagcaa
     1621 cacggccttc acgtcgggcg tttcattgaa accgtacacc gccttgacga ggaacaaacc
     1681 accgccgaaa tccttcaccg tcacgcggtc agcatcgttc acgtacggaa tgtcgcgcgt
     1741 cacgaaggtc aggaagatgg tgcgctcatg caacaccttg ttgtgcttca ggttgtgcag
     1801 caggctcacc ggcacgagcg aatcgctgcc ggtcagatag atcgccgtac cggacacgcg
     1861 gtgcggcgga tgcgcgagca aaccctgcag gaacggcatc aacggaatac cgtccgcggc
     1921 cgtgcgttcc ttgacgatca ttctgccctt gaaccaggtc atcagcagga agaacagcag
     1981 cgcgccgatg ccgagcggca gccagccacc ttcggcaacc ttgagcaggt tggcgccgaa
     2041 aaagcccagg tcgaccgcca tgaacgcgcc gatgatcaac cccacgagga atttgttcca
     2101 gttccacacg tttaccatca cgacacacgc gaggatcgta gtgatcacca tcgttgcagt
     2161 caccgcgata ccgtacgccg cagcgagatt atccgaactt ttgaacgcga tcacgatgca
     2221 taggatgatg aacaacagca tccagttcac gaccggcaca taaatctggc caatcgccag
     2281 ctccgacgta tgcaggatct tcatgcgcgg cacgtagccg agctggatgg cctggctcgt
     2341 cagcgaatac gcaccggaaa tcactgcttg cgatgcgatc acggttgcca ctgtcgacag
     2401 aacgacgagc ggcagaagcg cccaatccgg tgcgagcagg aagaacgggt tttcgatcgc
     2461 tttagggtcg cgcatcagca gcgcgccctg gccaaaatag ttcagcacta gcgacggcat
     2521 cacgaggaag taccaggcca tacggatcgg cttggcgccg aagtggccca tgtcggcata
     2581 cagcgcttcg gcgccggtca gcaccagcac gaccgagccg agcaccacgt aggcctgcag
     2641 cacgtgtgcc cccatgaacg tgtaagcgta atacgggttc agtgcgcgga tcacgttcgg
     2701 cgactgcagg atgtgccata ggcccagcgc ggcgagcacc aggaaccaca gcaccatgat
     2761 cggaccaaac aggcggccga cggtggccgt gccgtgccgc tgaatccaga acagcagcac
     2821 gaggatcacc atcgtgagcg gcaacaccag atgggacaga tggggagccg cgatctcaag
     2881 accttcgacc gccgagatca ccgaaatagc gggtgtaatc accgcgtcac catagaacat
     2941 gcaggcgcca aagatgccga gcatcatcag caagccggcc attttcgatt tgtcgtcgag
     3001 agagcgcagc gccagcgcca tgagtgcgag gacgccgcct tcgccgttgt tgtcggcgcg
     3061 catcacgaac agcacgtact tgacgccgac gacgatcacg atcgcccaga acagcagcga
     3121 tatcacgccc agaatcgatt gatcggtcag cggaatgcca tgcgccggac tgaaagcttc
     3181 tttcagcgag tacaacgggc tcgtgccgat atcgccgaac accaccccga tcgcggcaac
     3241 cgcaagggaa ggcaaaggct gtttgtgcac gtgattgtta tgtgtcatag acgcgaggaa
     3301 atccgttccc tgagcggaaa aaacgaccgc tattctaaac tgcccgccta gcgccgcccg
     3361 gcttgttgtg gatacaccac gtggatggtc cgcgcagtgt agcactgcga tttgggcgcg
     3421 tctgcggcgg caggtgattc gccggcgcag gcttgcgcgt tttgcgaagc accgttcaca
     3481 ggacgcatca cgaccctaca cgtcgcgcat aaaaaaacgg agccgaaccg ggctccgttt
     3541 tgcaggcact gcactggcga caacgcaata agtacggcgt cgcagcgcat ctcgtgctga
     3601 cctcacgtgc ctgacgactc ctgctcctgc tgcgtaccgc gcttgatcca cgcgccgagg
     3661 ttgtgcggac gaaccgtgtc ccattcctcg aacggctggt gaatccacgg attagtcggc
     3721 agatactgca cgtggtaatc gggcttcact ttcgagcagc ccttgtacca gagcaccgcc
     3781 gaacgcaccg cggtgatcgc cggataacgt tccttcagat gctgctgtac acgtgccagc
     3841 gttacgcccg aatcaaccag atcgtcgacc agcaacacgt tgccgtgcag ctcgccgcgc
     3901 gtcatcgtga tgtattgcgc gatgtcgagt tcgccctgct ccgtgccggc cgcttcgcgg
     3961 tacgaactgg tggcgagaat cgccagcggc agatcgcaaa tgcgcgagag ctggtcgccg
     4021 acgcgcaaac cgccgcgcgc gagacacagg atcttgtcga acttccaacc cgattcatgg
     4081 acggccaacg ccagcagttc gatcagccgg tgatactcgt cccagccgac ccacaggttc
     4141 ttgtcatcgt tgcgcggatc tttcatcgca atcatgggta caccttgaac catggcttaa
     4201 accttgaacg gatgacgcag cagaatcgtt tcgtcgcgat ccggaccggt cgacaccatg
     4261 tcgatcggaa tacccgcgac ttcctgcaca cgcgtcagat acgcacgcgc gctggccggc
     4321 agcttgtccc attccgtgat gccgacggtg ctttccttcc agcccgcgaa ggtttcgtac
     4381 accggcacgc aacgcgcgac ttccgatgcg ccacgcggga gcaggtcgac agtcttgccg
     4441 tcgaccgtgt agccgatgca cagcttcact tcgtccagac cgtcgagcac gtcgagcttg
     4501 gtcatgcaca gacccgacac gccgttgatc tggatcgagc ggcgcagcgc ggcggcgtcg
     4561 agccaacccg tgcggcgcgg acggccggtc accgagccga attccttgcc gaccttggcg
     4621 agttccaggc cgatcggttc ctggcgcgcg gcgttatccg cgtcgtacag ttcgctcggg
     4681 aacgggcccg aaccgacacg cgtgcaatac gccttggtga tgccgagaat gtaattcagc
     4741 ttttgcggac cgacgccggc gcccgcggta gccgcaccgg ccacgcagtt gctcgacgtg
     4801 acgaacggat aggtgccgtg gtcgatgtcg agcagcgtgc cttgcgcgcc ttcgaacagc
     4861 agattgttgc cggccgcgtt ttcgtcgtac agacggcgcg acacgtcggt caccatcggc
     4921 ttcagacgat cggcatagct cagcatcgtg tcgagcgttt gctggaaatc gaccgcggcg
     4981 acgcccaggt attgcgtcag cacgaagttg tggtaatcga ggttctcacg cagacgttcg
     5041 gcgaagcttt ccggctcgaa caggtcttgc acacgcaagc cgcggcgcgc caccttgtct
     5101 tcgtaggccg ggccgatgcc gcggccggtg gtgccgatct tgctcgcgcc acggcgcgct
     5161 tcgcggccct ggtcgatggc aatgtggtac ggcagaatca gggtggtggc ttcggaaatg
     5221 aaaaggcgat tctgaacgtc gacgccggcg gcttcgagct cgccaatttc cttgaacagc
     5281 gcttccggcg acaacacgac gccattgccg atgtagcacg cgacgccggg atgcat
//
LOCUS       DATASH010000462         4595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5414944,
            whole genome shotgun sequence.
ACCESSION   DATASH010000462 DATASH010000000
VERSION     DATASH010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4595
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5414944"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(718..1473)
                     /gene="cobA"
                     /locus_tag="VHV99_12780"
     CDS             complement(718..1473)
                     /gene="cobA"
                     /locus_tag="VHV99_12780"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018431718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEX3636345.1"
                     /translation="MGKVYLIGAGPGAADLITVRGARLLGLADVVLHDALVEPAMLDY
                     APSHARRIAVGKRCGQLSTAQQFINKQIVDAALEHDVVVRLKGGDPMLFGRADEEMRA
                     LEAAGIEYEVVPGITAALASAASLKRSLTLRGVSRSVALATFSRAPGSEEIREQVNAD
                     SLVFYMGRDSGVEIAQQLIDAGRSVSTPVAIVEACSTERERMLTLTLEALAAGEAQKW
                     VDASQPSLLMIGEAFAARQQVGRSAGEVLVAAA"
     gene            complement(1549..2862)
                     /locus_tag="VHV99_12785"
     CDS             complement(1549..2862)
                     /locus_tag="VHV99_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007582454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX3636346.1"
                     /translation="MSIHQPEDLGVLRFITAGSVDDGKSTLIGRLLYDSKAVLSDQLS
                     ALSRAKNKRTVGDEIDLSLLTDGLEAEREQGITIDVAYRYFATAKRKFIIADTPGHEQ
                     YTRNMVTGASTAHAAIILVDATRVTFVDGVAQLLPQTKRHSALVKLLGLQHAIVAINK
                     MDLVDYSETRFNEIRDAYVELARHLGLNDVRFVPVSALKGDNIVTASESMPWYAGEPL
                     LDLLEALPVEIPTGQALRFPVQWVARQDGSQADDFRGYMGRVEAGEVKVGDTIVVLPA
                     NREATVAEIIAPVIGGTAAVDRAFAGQTVTIRLAEDVDVSRGDTFVLREEAPEPARKL
                     EADLCWFDDTPLSTQRKYLLKQTTNTVFARIGAIKEVLDVHTLSQATDRKELAMNDIG
                     RVALTLQKPLVCDVYDSHQGTGAFVLIDEATHHTVAAGMIRAFSA"
     gene            complement(3030..3992)
                     /gene="cysD"
                     /locus_tag="VHV99_12790"
     CDS             complement(3030..3992)
                     /gene="cysD"
                     /locus_tag="VHV99_12790"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008351121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEX3636347.1"
                     /translation="MSTTLDSTINAPLTNTANRMDHLDWLEAESIHILRELVAECSKP
                     ALLFSGGKDSVVVLHLALKAFGIGANRKTVLPFPLVHIDTGHNYEEVIDFRDRRAKEI
                     GAELVVGHVEDSIKRGTVRLRRELDSRNAAQAVTLLETIAEHEYTALIGGARRDEEKA
                     RAKERIFSFRDEFGQWDPKAQRPELWSLYNARLHNGEHLRVFPISNWTELDVWQYIAR
                     EQLELPSIYYAHQREIVRRNGLLVPVTPLTPMREGETSETALVRFRTVGDISCTCPVE
                     SDADDLEKIIAETAVTEITERGATRMDDQVSEAAMEQRKKQGYF"
     gene            complement(4105..>4595)
                     /locus_tag="VHV99_12795"
     CDS             complement(4105..>4595)
                     /locus_tag="VHV99_12795"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013338449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEX3636348.1"
                     /translation="QFHPQAAAVEEYIGSHGLNAFYESVDLRKRCCEIRKVEPLNRAL
                     SDVSAWVTGQRREQSVTRAQLHEEEHDGARNIAKFNPLTDWTEAEVWDYLKAFDVPVN
                     PLHARGYPSIGCEPCTRAIRPGEDSRAGRWWWESRDTKECGLHITTITPIPVSSGENA
                     SA"
BASE COUNT          961 a         1503 c         1250 g          881 t
ORIGIN      
        1 tgttctcgcg catttcgcgt gagtgacaca gcagtcatac ttccggcggc gctgcgcgcg
       61 ccgaaggggg cttcgaaaaa acattgggtt tttggacatg tcgcctcaaa aacacttagt
      121 aaaaccaatg gtttcgcgcg atctccgtag aggatttcag aaagccaatc gctgtccgga
      181 cgcattgccg tggagcatat tcgcgccgag gcgaagccga cacccccaaa acaaagccac
      241 tcttcgtccg atgaaccctt ccggcgagca cgtagtgcga ggcgggaagg atgagtgctt
      301 tgtcactcac gccgaatgtg cgagaacaca gattccagat gcaccactgc cgtggctgtg
      361 cgagggaccc gcttatgagc tggcggtgtg agccgctttc ttttgcctac ttttctttgc
      421 ggcaggcaaa gaaaagtagg tgcctgcccc gcacaggggc gacgctaata aaccaataac
      481 aaatcaagga aaggccaacg ccgcaggcgt acaacccaaa agcgctgcgt aggcaaccag
      541 ctcaagaaaa gcccaacgcc gtaggcagac agataagcaa gcgcgagcag gcaaaaaaat
      601 caagcaaagc ccaacgccct aagcaaacag aaaataagcg ccgcgcaggc aaccaattca
      661 ataaagccca atgccgcagg caaacagaca atcaagcgcc gcgcaagaaa aaacccatca
      721 ggcagcagca accaaaacct cccccgcgga gcgccccacc tgctgccggg cagcaaaagc
      781 ctccccgatc atcaaaagac taggctgcga agcatcaacc catttctgcg cctcaccagc
      841 agcgagcgct tcaagcgtga gtgtgagcat ccgctcacgt tccgtactac aagcctcaac
      901 aatcgccacc ggcgtagaaa cactccgccc ggcatcaatc aattgctgag caatctcaac
      961 cccactatcg cggcccatat aaaacacgag ggaatccgca ttaacctgct cgcgaatttc
     1021 ttcagaccct ggagcacgac taaaagtagc cagcgcaaca ctacgagaca cgccgcgcaa
     1081 cgtaagagac cgcttcaacg aagccgcact agccagcgca gccgtaatcc ccggtaccac
     1141 ctcatactca atcccggcag cctccagcgc ccgcatctcc tcatcagcac gcccgaacaa
     1201 catcggatcg ccgcccttca gccgcaccac gacatcgtgc tccaacgcag catcaacaat
     1261 ctgcttgttg atgaactgct gcgcggtaga aagctgccca cagcgcttac ccacagcaat
     1321 ccgccgagcg tgcgaaggtg catagtcgag catcgcaggc tcaaccagcg cgtcgtgcag
     1381 cacgacatcc gccaagccaa gcagccgcgc gccgcgcacc gtgatgagat ccgcagctcc
     1441 cggccctgca ccgatcagat acaccttacc catttgcctc aaccccattc atccggttcg
     1501 tgcaaaaacc cgaagcagcg ggcgcgcgtc agtcacccag tcaacgactc aagcagaaaa
     1561 cgcccgaatc atcccagccg cgaccgtatg atgcgtcgcc tcgtcgatca gcacgaatgc
     1621 cccagtgccc tgatgcgagt cgtacacgtc gcacaccagc ggcttctgca acgtcagcgc
     1681 cacgcggccg atgtcgttca tcgccagttc cttgcgatcg gtcgcctgcg acagcgtatg
     1741 cacgtcgagc acttccttga tcgcaccgat ccgcgcgaac accgtgttgg tggtctgctt
     1801 caacagatac ttgcgttgcg tcgacagcgg cgtgtcgtcg aaccagcaca ggtcggcttc
     1861 cagtttcctg gccggttccg gcgcttcctc acgcagcacg aaagtatcac cacgcgacac
     1921 atcgacgtct tccgccagac gaatcgtcac cgtctgaccc gcgaacgcgc ggtccaccgc
     1981 agccgtgccg ccgatcaccg gtgcgatgat ctcggccacc gtggcttcac ggttcgccgg
     2041 cagcacaacg atcgtgtcgc cgacttttac ttcgcccgcc tcgacacggc ccatgtagcc
     2101 gcggaagtcg tcggcctggc tgccgtcctg gcgcgccacc cattgcaccg ggaaacgcag
     2161 cgcctggccc gtcggaatct cgaccggcaa tgcttcgagc aaatcgagca gcggctcgcc
     2221 tgcgtaccac ggcatgcttt cgctagccgt cacgatgttg tcgcccttca gtgccgacac
     2281 cggcacgaag cgcacatcgt tcagacccag atggcgcgcg agttcgacat acgcatcgcg
     2341 gatctcgttg aagcgcgttt cgctatagtc gaccagatcc atcttgttga tcgcgacgat
     2401 cgcgtgctgc aagccgagca gcttcacgag cgcgctgtga cgcttggttt gcggcagcaa
     2461 ttgcgccacg ccgtcgacga acgtcacacg cgttgcgtcg accaggatga tcgccgcgtg
     2521 cgcggtcgat gcgcccgtga ccatgttgcg cgtgtactgc tcgtgacccg gcgtgtcggc
     2581 gatgatgaac ttgcgtttgg ccgtcgcaaa gtagcgatac gcaacatcga tcgtgatgcc
     2641 ttgctcgcgc tcggcctcga ggccgtccgt cagcagcgac agatcgattt cgtcgccaac
     2701 ggtacgctta ttcttcgcgc gcgacagagc cgagagctgg tcgctcaaca ccgccttgct
     2761 gtcgtacagc aggcggccga tcaacgtgct cttgccgtcg tcgacgctgc ccgcggtaat
     2821 gaatcgcagc acgccgaggt cttctggttg atgaatactc atgatgttcc ttgtccgtct
     2881 gtcgaccaga gttagcgtgt cgaccggagt tggcgcttta gcgcttactc cggtcccata
     2941 gcgcttactc tggtcccatg caaagctgtc gactggagtt agcgtgtcga ccggagtagg
     3001 cgctctcgcg cctactccag tcccatgcat tagaaataac cctgcttctt ccgctgttcc
     3061 atcgcggcct cggacacctg atcgtccatc cgcgtcgcgc cgcgttccgt gatctcagtc
     3121 acggccgttt cggcgatgat cttctcgaga tcgtccgcat cgctttccac cgggcacgtg
     3181 cagctgatgt cgcccacggt acggaaacgc acgagcgcgg tttcgctggt ctcaccttca
     3241 cgcatcggcg tgagcggcgt aaccggcacg agcaggccat tgcggcgcac gatctcgcgt
     3301 tgatgcgcgt agtagatcga cggcaattca agctgttcgc gagcgatgta ttgccacacg
     3361 tcgagttcgg tccagttcga gatcgggaac acgcgcaggt gttcgccatt gtgcagacgc
     3421 gcgttgtaca ggctccacag ttccggacgc tgtgccttcg ggtcccattg gccgaactcg
     3481 tcgcggaacg agaaaatccg ttcttttgca cgggcctttt cttcgtcgcg gcgtgcgcca
     3541 ccgatcagcg cggtgtactc atgttccgcg atcgtttcga gcagcgtcac ggcttgcgcg
     3601 gcattgcgcg aatccagttc gcggcgcaga cgcaccgtgc cgcgcttgat cgaatcttcg
     3661 acgtgaccca ccaccagttc agcaccgatc tctttcgcgc gacggtcgcg gaagtcgatc
     3721 acttcctcgt aattgtggcc ggtgtcgata tgcacgagcg ggaacggcag cacggtcttg
     3781 cgattcgcgc caatgccaaa ggccttcagc gcaaggtgca gtaccacgac cgaatccttg
     3841 ccgcccgaaa acagcagagc gggcttgctg cattccgcga ccagttcgcg caggatgtga
     3901 atcgactcgg cttcgagcca atcgaggtga tccatccggt tcgccgtatt ggtaagcggt
     3961 gcgttgatag tggaatcgag cgtggtgctc atgtttgggt ccttcattgg ttcgcttgac
     4021 gagcggttcg ccccaagcag aagtattcaa atcggtatgt acagcgttca agcccaaaag
     4081 ccaaggcctg aactgccaaa tgctttatgc cgaagcgttt tcgccactgc tgacggggat
     4141 cggcgtaatc gtcgtgatat gcagcccgca ttccttcgta tcgcgcgact cccaccacca
     4201 gcgccctgcc cggctgtctt caccggggcg gatcgcacgc gtacaaggct cgcagccgat
     4261 gctcgggtaa ccgcgcgcat gcagcggatt gaccggcacg tcgaacgcct tcagataatc
     4321 ccacacttcg gcttcggtcc agtccgtcag cggattgaac ttggcaatgt tacgcgcgcc
     4381 gtcgtgttct tcctcgtgca gctgggcacg tgtcaccgac tgctcgcggc gttggcccgt
     4441 cacccacgcg ctgacatccg acagcgcgcg attcaacggc tcgaccttgc ggatctcgca
     4501 gcaacgcttg cgcagatcga cgctttcgta gaacgcgttc agaccgtgcg agccaatgta
     4561 ttcctcgact gccgcagctt gcggatggaa ctgtt
//
LOCUS       DATASH010000463         4359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5809997,
            whole genome shotgun sequence.
ACCESSION   DATASH010000463 DATASH010000000
VERSION     DATASH010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4359
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5809997"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VHV99_12800"
     CDS             <1..380
                     /locus_tag="VHV99_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013338897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HEX3636349.1"
                     /translation="KGLRLIMMCEVPSNAILAEEFLQFFDGFSIGSNDLTQLTLGLDR
                     DSGMELLAVDFDERDPAVKFMLKRAIETCLRLNKYVGICGQGPSDHPDFAQWLTDEGI
                     ASISLNPDSIIETWQQLAKSLAK"
     gene            547..1002
                     /locus_tag="VHV99_12805"
     CDS             547..1002
                     /locus_tag="VHV99_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEX3636350.1"
                     /translation="MAPGGLFWWIGAGVLVVLELISGTFYLLMIALGCVAAAIAHMAG
                     AAADLQFAIAAVVALVAVLLLRRSRFGRRTRKEASKNPDVNLDIGQTLSVPAWHERRA
                     RVNYRGAAWDVELAAGEPEDAQSYEIVEMRGSCLVVAASRQAKAKATAA"
     gene            1077..2009
                     /locus_tag="VHV99_12810"
     CDS             1077..2009
                     /locus_tag="VHV99_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX3636351.1"
                     /translation="MDSTIVAAVLLIIVIVLAAQTLKIVPQQHAWVLERLGRYHRTLT
                     PGLSFAFPFVDRIAYKHILKEIPLEVPSQVCITRDNTQLQVDGVLYFQVTDPMKASYG
                     SSNFVFAITQLSQTTLRSVIGKLELDKTFEERDFINHSIVSSLDEAAQNWGVKVLRYE
                     IKDLTPPKEILHAMQAQITAEREKRALIAASEGRKQEQINIASGGREAAIQKSEGERQ
                     AAINQAQGQASAILAVAEANSQAIQKIAAAIQSNGGMEAVNLKVAEQYVNAFGNLAKQ
                     GTTLIVPGNLADMSSMIASALTIVNKGKGASDVR"
     gene            complement(2086..2532)
                     /gene="smpB"
                     /locus_tag="VHV99_12815"
     CDS             complement(2086..2532)
                     /gene="smpB"
                     /locus_tag="VHV99_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004193190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEX3636352.1"
                     /translation="MSIIDNRKAFYDYSVEERYEAGLVLEGWEVKALRAGRGQIKEGY
                     VVIKNNELFLIGTHISPLPEASTHIHPDPVRTRKLLLHSEEISKLIGKVEQRGYTLVP
                     LNFHYKGGRVKCEIGLAKGKKQHDKRETEKKRDWEREKARLMRSPS"
     gene            2645..3082
                     /locus_tag="VHV99_12820"
     CDS             2645..3082
                     /locus_tag="VHV99_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEX3636353.1"
                     /translation="MADVQKTVLIRHSAEQMFDLVTDVADYPNFLPWCGGVEIRRQDE
                     TGMEAKIDINFKGIKQHFATRNSMERPTRIDMEFADGPFRKFTGFWRFTPLRADACKI
                     EFALHYEFTNIILEKIIGPVFSHIANTFVESFVKRADQRYGKA"
     gene            3079..3411
                     /locus_tag="VHV99_12825"
     CDS             3079..3411
                     /locus_tag="VHV99_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HEX3636354.1"
                     /translation="MSARLSIEVCYALAGEQTLIAVELPEGATLQQALEASGILQRYP
                     RIDLGTQKVGVFGKLKPLDTVLADHDRVEIYRPLLVDPKLSRQRRVEKTRKAGSIEGR
                     RWQNKDSR"
     gene            complement(3434..4357)
                     /locus_tag="VHV99_12830"
     CDS             complement(3434..4357)
                     /locus_tag="VHV99_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007588236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX3636355.1"
                     /translation="MVPRVLPDFSPLLMSAGRYTMYGIVSLAAALPMARSLIKRLTRE
                     DLGALVKLALAGNLLYYLLLASAVHMIGIAPASLIVGVLPVTVTLLGRRDHGAVPLAR
                     LAWPLLLVVAGIACINVDVFTVADSTQASIATKLLGLACALGALACWTWFAVENARYL
                     QRQTHFSGNEWSVLWGVVTGALGAGLWLVVAVMPSGSLQGPLGDARWHTFWMLNLGLA
                     VGASWLGNGLWNAASKRLPLTLSGQMIVFETLFALLYAFIYDQRLPRPLETAAILLLV
                     AGVCWSVRQHAGDDDTSGAASIEEKAQPSVH"
BASE COUNT          863 a         1288 c         1404 g          804 t
ORIGIN      
        1 agaagggcct gcgcctgatc atgatgtgcg aagtgccgtc gaacgcgatt ctcgccgaag
       61 agttcctgca attcttcgac ggtttctcga tcggttcgaa cgatctgacg cagctcacgc
      121 tcggcctcga ccgcgattcc ggcatggaac tgctcgcagt cgatttcgac gaacgcgacc
      181 cggcagtcaa gttcatgctc aagcgagcga ttgaaacctg cttgcgtttg aacaagtatg
      241 tcggtatctg cggccagggt ccttcggatc atccggactt cgcgcaatgg ctgaccgatg
      301 aaggcatcgc gtcgatctcg ctgaacccgg attcgatcat cgagacgtgg cagcagctgg
      361 cgaaatcgtt ggcgaaataa gcgatgtgaa taagcgcgat gcaggtgcct gataagccgc
      421 ttgcccaaca agcggtgcgg tatgtcaaaa gttcgtaaaa gcgtactgca ccgcccaatc
      481 aaggcacccg cttcatgcta taaacacccc ggtagatccg gggtgttttg ctttgtggag
      541 gtcgacgtgg cgccaggtgg attgttctgg tggatcgggg cgggtgtgct tgtcgtgctg
      601 gaactgatca gcggcacctt ctatttgcta atgatcgcgt tgggctgcgt cgcggcggct
      661 atcgcgcaca tggcgggcgc cgccgcggat cttcaattcg cgatcgcagc cgtggtggcg
      721 ctggtggccg ttctgttgct caggcgttcg cgctttggcc gcagaacgcg taaagaagcg
      781 tcgaagaacc cggacgtcaa tctggacatc ggtcagacgc tgagcgtgcc ggcgtggcac
      841 gagcgccggg cgcgcgtcaa ctaccgcggg gcggcgtggg acgtcgagct ggccgccggc
      901 gagcctgaag atgcgcagag ctacgaaatc gtcgagatgc gcggcagttg cctcgtcgtc
      961 gcggcgagcc ggcaggcaaa ggccaaagcg acggcggcct gagcgcgcac taacacgtgc
     1021 acggcgcgtg ctaagcatca cgcacaacta ttcgaactac ggaccggagg gcagtaatgg
     1081 attcaaccat cgtcgcggcg gtgcttctga tcatcgtgat cgtgctggcg gcacagacgc
     1141 tgaagatcgt gccgcagcaa catgcgtggg tgctggagcg gctcggccgc taccaccgta
     1201 cactgacacc gggcttgagc ttcgccttcc cgttcgtcga ccggatcgcg tacaagcaca
     1261 tcctcaagga aatcccgctc gaagtgccga gccaggtgtg tattacgcgt gacaacacgc
     1321 agttgcaggt ggacggcgtg ctgtattttc aggtcaccga tccgatgaaa gcgtcgtacg
     1381 ggtcgagtaa tttcgtgttc gcgatcacgc agctgtcgca gaccacgctg cgctcggtga
     1441 tcggcaagct cgagctcgac aagaccttcg aggagcgcga cttcatcaac cacagcatcg
     1501 tatcttcgct cgatgaggcg gcgcagaact ggggtgtgaa ggtgctgcgc tatgagatca
     1561 aggacctgac gccgcccaag gaaattcttc acgcaatgca ggcgcagatt actgctgagc
     1621 gcgagaagcg tgcactgatc gcggcatccg aagggcgcaa gcaggagcag atcaatatcg
     1681 cttcgggtgg gcgcgaagcg gcgattcaga agtccgaggg cgagcgccag gcggcgatca
     1741 atcaggcgca ggggcaggcg tcggcgattc tggcggtggc cgaagcgaac tcccaggcga
     1801 ttcagaagat cgccgcggcg atccagtcga atggcgggat ggaggcggtg aatctgaagg
     1861 tggccgaaca atacgtcaat gcgttcggca atctggcgaa acagggcacc acgctgatcg
     1921 tgccgggcaa cctggcggat atgagttcga tgatcgcgtc ggcgctgacc atcgtgaaca
     1981 aggggaaggg cgcgagcgac gtgcgctgag gccggttttt gctgccccaa ggaaaatggc
     2041 ccgctgactg agcgggccat ttttgtttga ggctcaaacg caggatcacg acggcgagcg
     2101 catcaggcgc gccttctcgc gttcccagtc gcgcttcttt tcggtttcgc gcttgtcgtg
     2161 ctgcttcttg cccttggcca gcccgatctc gcatttgacg cggccgccct tgtaatggaa
     2221 gttcagcggc acgagcgtgt agccgcgctg ctcgactttg ccgatcagtt tgctgatttc
     2281 ctcgctgtgc aagagcagct tgcgcgtgcg taccgggtca gggtggatgt gggtcgaggc
     2341 ttcgggcagc gggctgatgt gcgtgccgat caggaacagt tcgttattct tgataacgac
     2401 gtaaccttcc ttgatctggc cgcgcccggc gcgcagcgcc ttgacctccc atccttcgag
     2461 cacgagccct gcttcatagc gttcttcgac cgagtagtcg tagaaggctt ttctgttgtc
     2521 gatgatgctc atgaatggaa agtgcccaac tcgtttaaaa tcacgatttt agcaaagcgg
     2581 ggcaaggaaa gcccgcggcg cttttcaacc cgcttgtcca cccttgccac gcgctgttca
     2641 atttatggca gatgtccaga aaaccgtgtt gatccgccat tcggcggaac agatgttcga
     2701 cctcgtcacc gacgtcgccg attaccccaa cttcctgcct tggtgcgggg gagtcgaaat
     2761 ccgccgccag gacgaaaccg gcatggaggc gaagatcgat atcaacttca agggcatcaa
     2821 gcagcatttc gccacgcgca acagcatgga gcggcccacg cgcatcgata tggaattcgc
     2881 ggacggcccg tttcgcaaat tcaccggctt ctggcgtttc acgccgttgc gcgcggacgc
     2941 ctgcaagatc gaattcgcgc tgcactacga attcaccaac atcattctcg agaagatcat
     3001 tgggccggtg ttcagccaca ttgccaacac cttcgtcgaa tcgttcgtga agcgcgccga
     3061 tcagcgctac ggtaaggcat gagcgcgcgc ttgtcgattg aagtctgcta cgcgctcgcg
     3121 ggtgagcaga cgctgatcgc cgtggaattg ccggaaggcg ccacgctgca gcaggcgctc
     3181 gaggcgagcg gcatcttgca gcgctatccg cggatcgatc tgggcacgca aaaggtcggc
     3241 gtgttcggca agctcaagcc gctcgatacg gtgcttgccg atcacgatcg cgttgagatt
     3301 taccggccgc tgctggtgga cccgaagctg tcgcggcaac ggcgcgtcga gaagacccgc
     3361 aaggcgggct cgattgaagg gcgccgctgg cagaacaagg attcgcgcta ggcgagtctt
     3421 gtggcggcgc ctgtcaatgc accgaaggct gcgccttttc ttcaatcgat gccgcaccgg
     3481 acgtatcgtc gtcgcccgca tgctggcgca ccgaccagca cacccccgcc accagcagca
     3541 ggatcgccgc cgtttccagc ggacgcggca ggcgctggtc gtagataaac gcatagagca
     3601 gcgcgaatag cgtctcgaac acgatcatct ggccggacag ggtgagcggc agccgtttcg
     3661 acgcggcgtt ccacagcccg ttgccgagcc acgatgcgcc gactgcgagg cccagattca
     3721 acatccagaa ggtatgccag cgcgcgtcgc cgagcggccc ctgcaggctg ccggagggca
     3781 tgacggcgac caccagccac aagcccgcgc cgagcgcgcc ggtcacgaca ccccatagca
     3841 ccgaccactc gttgccgctg aaatgcgtct gacgttgcag gtagcgggcg ttttcgaccg
     3901 cgaaccaggt ccagcaggcg agtgcgccga gcgcgcaggc gaggccgagg agtttcgtcg
     3961 cgatgctcgc ttgcgtgctg tccgccaccg tgaagacgtc gacgttgatg caggcgatgc
     4021 ccgcgacaac cagcaggagc ggccatgcga gacgagcgag cggcacagcg ccgtgatcgc
     4081 gccggccgag cagcgttacc gtgacaggca gcacgccgac gatcagcgag gctggcgcga
     4141 tgccgatcat atggaccgcc gaggccagca gcaggtagta gagcaggttg ccggccaatg
     4201 ccagcttgac gagcgccccg agatcctcgc gggtcaggcg tttgatgagc gagcgcgcca
     4261 tcggcagcgc ggcggccagc gagacgatgc cgtacatcgt gtagcggccg gcgctcatca
     4321 gcagcggcga aaaatcgggc agcacgcgcg gcaccagaa
//
LOCUS       DATASH010000464         9293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5810006,
            whole genome shotgun sequence.
ACCESSION   DATASH010000464 DATASH010000000
VERSION     DATASH010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 9293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9293
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5810006"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            901..1467
                     /locus_tag="VHV99_12835"
     CDS             901..1467
                     /locus_tag="VHV99_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007744767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEX3636356.1"
                     /translation="MIRLPLAAITHACMASLLVVATAASAQTAPAAPSAAAPDAGRLH
                     AADQAFIADAARNVATQRDAARIATSRSTDRDVKAFAERVSSDNAKISDALRAASPRG
                     VDVPKNDPDAAVLASINNLRGADFDKTYIEQVALAGEQKALSAFQAEIASGRDEQLKE
                     AAKKALPTIQEHYAMAQDLAKRKHLTVQ"
     gene            1845..3572
                     /locus_tag="VHV99_12840"
     CDS             1845..3572
                     /locus_tag="VHV99_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing
                     glycosyltransferase family protein"
                     /protein_id="HEX3636357.1"
                     /translation="MSKPANFPQQLDHMLRQAIALQHNGAFAEAEELYREILELKPRH
                     FDALQLLGSLALQGGRLQEGIELIRRALAINARQAPLHSNLAYALNTLQRFDEALACA
                     DRALVLQPKFPDALNNRGNAQAGLNLPLDALASFDRAIVLTPDFAQAWNNRACVLRDL
                     DRPADALASCDHALALQPNYPDAWSNRGNALSDLNQPQEAERSYRRALELAPAFADAW
                     NNLGLTQIDLNQHAQALSSYARALAVNPAAAETHWNESLCLLQTGQLETGWQKYEWRW
                     ERPRIKASKRAFAQPLWRGDFSIDGKTILLHAEQGLGDTLQFCRYAALVSKLGAKVVL
                     EVPSELMRLMSTLHGVDQLIEAGQALPPFDCHCPLLSLPLAFNTDLASIPAKTPYLFA
                     DPHATREWDARIAAQAQGRLKVGLVWAGGHRPHVAELRKNDARRSITFERLAPVLDVP
                     NVQFFSLQKGPAALQLARNDPHARVVDYTEELDDFADTAALVANLDLVISVDTSTAHL
                     AGALDKPVWILNRFDSCWRWMLKRTDSPWYAQARLFRQPALGDWDSVIRSARDALAAL
                     CAAQKPTAQ"
     gene            3756..4781
                     /locus_tag="VHV99_12845"
     CDS             3756..4781
                     /locus_tag="VHV99_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX3636358.1"
                     /translation="MEYRHLGASGFKVPVLSFGTGTFGGKGEFFQAWGATDVAEARRL
                     IDICFDSGVTMFDSADIYSSGASESVLGEALKGKRDKAIISTKATFRFDDGPNNVGSS
                     RFHLIQAVDAALKRLQTDYIDLFQLHGFDAKTPVAEVMSTLDDLVRAGKIRYTGVSNF
                     SGWHLMKSQDTADRYGYPRYVANQTYYSLIGRDYEWELMPLGADQGVGAVVWSPLGWG
                     RLTGKIKRGQPLPETSRLHKTSDMGPPVPDEYLFRVLDAIDEVAAETGKTVPQIALNW
                     LLQRPTVSTVLIGARNEEQLRQNLGAVGWNLTPEQVAKLDAASAVRPAYPYWHQEGFA
                     ERNPKAV"
     gene            complement(5031..5774)
                     /locus_tag="VHV99_12850"
     CDS             complement(5031..5774)
                     /locus_tag="VHV99_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3636359.1"
                     /translation="MNTIKRAQVAIVTGASRGIGSAVAQRLAKDGFAVAVNYASSSTE
                     ADALVAGLTAEGTRAIAVKADVSKADDVRRMFETTEQQLGKVDVLVNNAGVLKTVPLA
                     DTSDALYDQTFDINVRGTFNTLREAAARMNDGGRIVNFSSTTLALNMPGYAIYNGTKA
                     AVEAFTHVFAKELRGRNITVNAVAPGPIATSLFLDGKTEEQIQTFAKMPPLQRLGQPD
                     DIASVVSFLAGPDAAWVNGQILRANGGVA"
     gene            5914..6828
                     /locus_tag="VHV99_12855"
     CDS             5914..6828
                     /locus_tag="VHV99_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3636360.1"
                     /translation="MDRFEEMRVFVRIAERQSFTRASDDLQIPRATVTNLMKRMEQRL
                     GARLLERTTRSVRLTHDGEAYYRRCVRLIADMEEAEGSFSNLAPKGLLRVNLQGTLAR
                     HFIVPALPAFLARFPDIELTIGEDDRLVDLVREGIDCVLRAGNLQDSSMVGRRVAQLP
                     QVTVASPAYLQAYGEPGDPTDLSTHRAVNYLSSATGKPVPLEFRVDGRDTATYLPWAV
                     SVTGADLYTGAAVAGLGIVQVPQYRVAGELASGRLKIILADFPPPPMPVSVLYPQNRQ
                     LSSRVRVFAQWLRDIFEAASVQAEARGS"
     gene            7039..7344
                     /locus_tag="VHV99_12860"
     CDS             7039..7344
                     /locus_tag="VHV99_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX3636361.1"
                     /translation="MIDGLVGGRLYGEAQIRTGQNGKRFVTCKVRATTNDGDTVFVNV
                     IAFDDDVQTALLALTDSDSVALSGALTPKVWTDKNGLIKAAIDMVAHKVLVGYRGEG"
     gene            8743..>9293
                     /locus_tag="VHV99_12865"
     CDS             8743..>9293
                     /locus_tag="VHV99_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEX3636362.1"
                     /translation="MYRHRSLGVVTGASPLAGVDVLSRMTAAWRRPGAARPFDFVLEQ
                     QSWQGPASLSGASAPFKIHVFDTIRAFEKRGVDAIVLPCFLSHTFIDELSANVTLPIA
                     NLMTALVAHLRQAFPSLRRIGVLTSDTIRSNGLFERYFDSAGFDVLHPRNEAGVDRVT
                     SAVYGDEGIKGGHLYGRPVELLRA"
BASE COUNT         1899 a         2696 c         2901 g         1797 t
ORIGIN      
        1 ctgtcatact tcccgcctcg ccctgcgtgc tcgccagaag ggttcatcat gcaaaccagc
       61 ggcttcgttt gggagcaggt gggagccatc ggccttgtct cggcgatgcg ccctggtgac
      121 ggcggcgaga gacgagaaga cagaagaggc gaaggggcgc gtgcacgccg cgccaagtga
      181 agcgtgagcg cgaaagcgcg gtgagcggtt ttaagaagtg cccttcggcg cgcgcagcgc
      241 cgccggaagt atgacggctg tgtcactgac gccgaatgtg cgagggcacg gattccagat
      301 gcaccactac cgcgactgtg cgggggaccc gcttatgggc tggcggtgtg agccgctttc
      361 ttttgcctac ttttctttgc ggcaggcaaa gaaaagtagg tgcctgcccc gcacaggggc
      421 gacgctaata gaccactatc aattcaagga aaggccaaag agccagatca aggaagacca
      481 aagagccaga tcaaggaaag gccgacgcca taggcagaca gacactaagc gccgagcagg
      541 caaccagatc aagcaaagcc caacgccgca ggcatccttg ttatcggcgc aatagcgaga
      601 aagttcagtg aattcaggcg ctctaccgat gttttccaat taacgcgtaa acgcttccat
      661 tcgggggtgg gaaggagaaa gtcagcccac gccagcaaat tcatatgaag cttacctaac
      721 gttacagaag cgacagcata gtttcacata ctcgataacc cgccccctag aatgcaaagc
      781 aataagagcg cggcaccgtt agccaaccgc aagcgaactg caagatcaca atacattcgc
      841 ccgcaatggc caaggtgcac ccgcatcgtc gtcgtttcgc ttccattcca aggagaacgt
      901 atgatccgct tgcctcttgc cgctattacc catgcctgta tggccagcct tctggtcgtc
      961 gctaccgcgg ccagcgcgca aaccgcgccc gccgctccgt cagcagcggc gccagacgca
     1021 ggtcgcctgc atgcggccga tcaggccttc atcgccgatg ccgccaggaa cgtcgccacg
     1081 caacgcgatg ccgcacgcat cgccacgtcg cgctccaccg accgtgacgt caaggcgttc
     1141 gccgaacgcg tgtcgagcga caacgcgaaa atctcggacg cgctgcgcgc cgcgagcccg
     1201 cgtggtgtcg acgtgccgaa gaacgatccg gacgccgcag tgctcgcgag catcaacaac
     1261 ctgcgcggcg ccgacttcga taagacctac atcgaacagg tcgcgctcgc cggcgaacag
     1321 aaggcgctgt cggcattcca ggcggaaatc gcctcaggcc gcgacgaaca gttgaaggag
     1381 gcggcaaaga aggcgctgcc gaccatccag gaacactacg cgatggcgca ggatctcgcc
     1441 aaacgcaaac atctgactgt ccagtaagct gccgactcga ggcacggtaa ggcaccagcg
     1501 cgcgtcacgt tgttgcgtga cgcgcgtgac cgcgagatca aaagcggctc gaaaggccgc
     1561 tcgaatagcc gccgaatagc cgcccaaatg tcgcccaaaa ttcactcgag aaaccgctcg
     1621 agaaaccact cgaaaagtgg cccgaaaagg cgcccccaaa tcccgccaca atgttttcgc
     1681 tcgcggtcaa cgctgcccgc atgcgcacag caaaccgaat gcattctttt cagcgataat
     1741 cggcttcgtt ctttcgagcc cggtctgacc tgcgccgccc agcgcgactc aaacagctcg
     1801 aaagccgttc ccgattccac gcgcattcat tcaaagctcg cctcatgagc aagcctgcca
     1861 atttcccgca acagttggac cacatgcttc ggcaagccat cgcgcttcag cacaacggcg
     1921 cgttcgccga ggccgaagag ctctaccgag agattctcga actcaaacca cgccatttcg
     1981 acgcgctgca attgttgggc tcgctcgcgc tgcaaggggg tcgtctgcaa gagggcatcg
     2041 agttgatcag aagggcgttg gccatcaacg cgagacaggc gccacttcat tcgaatctcg
     2101 cgtatgcgct caataccctg caacgtttcg acgaagccct tgcgtgcgcc gatcgcgcgc
     2161 tcgtattgca gcccaaattc ccggatgcac tgaacaatcg cggcaacgcg caggccggtc
     2221 tgaatttacc gctcgacgcg cttgccagct ttgatcgcgc gatcgtcttg acgccggatt
     2281 tcgcgcaggc ctggaacaat cgcgcctgcg tgctgcgcga tctggaccgt cccgccgatg
     2341 cattggcaag ttgcgaccac gcgctcgcac tgcagccgaa ctatcccgat gcatggagca
     2401 atcgcggcaa tgcgctcagc gatctcaatc agccgcaaga agcggagcgt agctatcggc
     2461 gcgcacttga actggcgccg gccttcgccg atgcctggaa taatctcggc ctgacccaga
     2521 tcgatctcaa ccagcacgct caagcgttgt cgagctacgc gcgcgcgctg gcggtgaatc
     2581 ccgcagcagc ggaaacgcat tggaacgaat cgctttgcct gttgcaaacg gggcagcttg
     2641 agactgggtg gcagaaatac gaatggcgtt gggaacgccc acgcatcaag gcgagcaagc
     2701 gtgccttcgc acaacccctt tggcgggggg atttttcaat cgacggcaaa acgattctgc
     2761 tgcacgccga gcagggcctc ggcgacacgc tgcagttttg ccgctatgcc gcgctcgtgt
     2821 cgaagctcgg cgccaaagtc gtattagaag taccgtccga attgatgcgg ctgatgtcca
     2881 cgctccacgg cgtggatcaa ttgatcgaag cggggcaggc actgccgccg ttcgattgtc
     2941 actgccctct actgagcctg ccgcttgcgt tcaacaccga tctcgcgagt attcccgcaa
     3001 agacaccgta tctgttcgcc gacccgcacg caacgcgtga atgggacgcg cgtatcgcag
     3061 cacaagcgca agggcgcttg aaagttggac tcgtctgggc cggcggacat cggccgcatg
     3121 tcgccgagct tcgcaagaac gacgcgcgcc gttcgattac gttcgagcgg ctcgcgccgg
     3181 ttctcgatgt gccgaatgtg cagttcttca gcctgcagaa aggcccggcc gcgctgcaat
     3241 tagcgcgcaa cgatccgcac gcgcgcgtcg tcgattacac agaagaactc gatgactttg
     3301 ccgacaccgc cgcgttggtg gcgaacctcg acctggtgat atcggtggac acgtccaccg
     3361 cgcatctggc cggtgcgctg gacaagccgg tgtggattct gaaccgcttc gacagctgct
     3421 ggcgctggat gctgaaacgc accgattcgc cctggtatgc gcaagcgagg ttgttccggc
     3481 agccggcatt gggcgactgg gacagcgtaa ttcgaagtgc gcgtgatgcg ctggccgcat
     3541 tgtgcgccgc gcaaaagcca acggcgcaat gaagataagg cgctgtctgt gcgttagccg
     3601 gacaggtcgg aactggacgc ttcactcgct gccatgacag cgctctttaa tccgcacgac
     3661 gcctgtcaca ccggcgcgag cccgccggca cgtttgccca gacgcgcgta gactgaacgt
     3721 ccgtttatcg atggctacgc cacggagatt gatcaatgga atacagacat ctgggtgcat
     3781 ccggtttcaa ggtgcccgta ctgagtttcg gcacgggcac attcggcggc aagggcgaat
     3841 tttttcaagc gtggggcgcc accgacgtcg ccgaagcacg tcgcctgatc gacatctgtt
     3901 tcgattcagg cgtcacgatg ttcgacagcg cggacatcta ttcgagcggc gcttccgagt
     3961 cggtgctcgg cgaagcgctc aagggcaagc gcgataaagc gatcatttcg accaaggcga
     4021 cctttcgttt cgacgacggt ccgaacaacg tcggctcgtc acgttttcat ctgatccagg
     4081 cggtggacgc ggcgctcaaa cgtttgcaaa ccgattacat cgacctgttc cagttgcatg
     4141 gtttcgacgc aaagacgccg gtcgccgaag tcatgtcgac gctcgacgat ctcgtgcgcg
     4201 cgggcaagat ccgctacacg ggcgtgtcga atttttccgg ctggcacctg atgaaatcgc
     4261 aggacaccgc ggatcgttac ggctatccgc gctacgtcgc gaatcagact tactattcgc
     4321 tgatcggccg cgattacgag tgggagttga tgccgctcgg cgccgatcaa ggcgtgggtg
     4381 cggtggtgtg gagtccgctc ggctgggggc gtctcaccgg caagatcaag cgcggtcagc
     4441 cgttgccgga aacgagccgt ctgcataaaa cctccgacat ggggccgccg gtgccggacg
     4501 agtatctgtt ccgcgtgctc gatgcgatcg acgaagtcgc ggccgaaacc ggcaagaccg
     4561 tgccgcaaat cgcgctgaac tggctgctgc agcgtcctac cgtttcgacg gtgctgatcg
     4621 gcgcgcgtaa cgaagaacag ttgcggcaga acctcggtgc agtgggctgg aatctgacgc
     4681 ccgaacaggt ggcgaagctc gatgctgcca gcgctgtacg tcccgcgtat ccgtactggc
     4741 atcaggaagg atttgcggag cgtaatccga aggcggttta agggcgctgc ctggcgtatc
     4801 caccgagtaa caactcgctc cggctcgact tgctacttga cgagttcggc cggagcggcg
     4861 agcagttcga tgtagtaccc atttccccgc gcttgcatgg gccgccggac acgactgtcg
     4921 gccgtattcg atttgccagt ccttcgcgtt ggcgagcgcc cgcattcctc ggcgcgtggc
     4981 ttgttgcgtc cttcgatatc gcgcatcggc gcgtagacga tatggccggc ttaagccaca
     5041 ccgccgttgg cgcgcaggat ctggccattg acccaggctg catccgggcc ggctaggaag
     5101 gacaccaccg aggcgatgtc gtcgggctgg ccgaggcgtt gcagcggcgg catcttcgca
     5161 aaggtctgaa tctgttcttc agtcttgccg tcgaggaaca gcgaggtcgc gatcgggccc
     5221 ggcgcgactg cgttgacggt gatgttgcga ccgcgcagtt ccttggcgaa cacgtgcgtg
     5281 aatgcctcga cagccgcctt ggtgccgttg tagatcgcat agcccggcat gttcagcgcc
     5341 agcgtggtgc tcgagaagtt gacgatgcgt ccgccgtcgt tcatccgtgc ggccgcttcg
     5401 cgcagagtgt tgaaggtgcc gcgtacgttg atgtcgaaag tctggtcgta cagtgcgtcg
     5461 ctggtgtcgg cgagcggcac cgtcttcagc acgccggcat tgttgaccag cacgtccact
     5521 ttgccgagct gctgttcggt agtctcgaac atgcgacgca cgtcgtcggc tttcgatacg
     5581 tcggccttca ccgcgatggc cctggtgcct tctgccgtga gtccggcgac gagcgcatca
     5641 gcctccgtcg aactcgacgc atagttgacg gcaaccgcaa aaccgtcctt ggcgaggcgt
     5701 tgcgcaacgg ctgagccgat gccgcgggat gcgccggtaa caatggcgac ttgagcacgc
     5761 ttgatcgtgt tcatgattcg tttccttttc cgtggtgggg tggtgagatg aatcatggcc
     5821 gctttccccg ctgagataat cggcttagac ttgccataat aattccattt actttaacaa
     5881 tcggccggca ggcctctcat agaggcgttg aacatggatc gtttcgagga aatgcgggtg
     5941 ttcgtgcgga ttgctgaacg gcagagcttc acgcgcgctt cggacgatct gcagattccg
     6001 cgcgccaccg tcaccaattt gatgaagcgc atggagcagc ggctcggcgc gcggctgctc
     6061 gagcgcacaa cgcgctcggt gcggcttacg catgacggag aggcgtacta ccggcgctgc
     6121 gtgcggttga tcgccgatat ggaggaggcg gagggctcgt tttccaacct cgcgccgaag
     6181 ggcctgttgc gcgtgaatct gcagggcacc ttggcgcggc acttcatcgt gccggcgctg
     6241 ccggcgtttc tcgcgcggtt tcccgacatc gagctgacga tcggcgagga tgaccggcta
     6301 gtcgatctgg tgcgcgaagg gatcgattgc gtgttgcggg cgggcaatct gcaggattcg
     6361 tcgatggtgg ggcggcgcgt ggcgcagctg ccgcaagtga cggtggcgag tccggcttat
     6421 ctgcaggcgt atggcgagcc aggcgatccg acggatttgt ccacgcatcg ggcggtcaat
     6481 tatctgtcca gtgcaaccgg caagccggtg ccgctcgaat tcagagtcga cggtcgcgat
     6541 acggcgacct acttgccgtg ggcggtgtcg gtgaccggcg ccgatctcta cacaggcgcg
     6601 gcggtagcgg ggctcggcat cgtgcaggtg ccgcagtacc gtgtggcggg agagctggca
     6661 tcggggcggt tgaagatcat cctcgcggac tttccgccgc cgccgatgcc ggtttcggtg
     6721 ctgtatccgc agaatcgtca gttgtcgtcg cgggtgcgag tgtttgcgca gtggttgcgc
     6781 gatatttttg aggccgcgag tgtgcaagcg gaggcgcggg gatcatgaag gacgcgtgct
     6841 gcgatgatga agtgagtagt caaggcaact acggtttgcc aggcgactga tgcgcggtgt
     6901 cgatatgatc cgcatgatgc gcgcgggcgg tgcgttgcct ggtgcgaggg ggtggcttat
     6961 cgtgggatca tgtgcgtcgt gctgggcgat attgtttttt gcacggtacg gacagtttgg
     7021 aataacagga gtagagcgat gatcgatgga ctggtgggcg ggcgattgta cggcgaggcg
     7081 cagattcgca caggacagaa cgggaagcgg ttcgtgacct gcaaggtgcg ggcgaccacc
     7141 aacgatggcg atacggtttt cgtcaatgtg attgcgtttg atgatgatgt gcagacggcg
     7201 ctacttgcgc tgactgattc agatagtgtc gcgctgagtg gggcgctgac gcccaaggtt
     7261 tggactgaca agaatgggtt gattaaggca gctatcgata tggttgcaca taaagtgctt
     7321 gtggggtatc gaggggaggg gtgatgtttt tttttgcctt tggcggcggt gttgattgtt
     7381 gtactgtggg tgtgattttt tttggttttt ttgcctgcgc ggcggttggt tgtctgtctg
     7441 cctgtggggt tggcctttcc ttgaactggc tctttggcct ttccttgaat tgatattggt
     7501 ctattagcgt tgcccctgtg cggggcggca cctacttttc tttgcctgcc gcaaagaaaa
     7561 gtaggcaaaa gaaagcggct caccccgcca gcgcataagc gggtcccccg cacagtcacg
     7621 gtagtggtgc atctggaatc tgtgccctcg cgcattcggc gtgagtgacc acgcgctcat
     7681 cagctcccac tccgcactgc gtgcgtcgcg gatgggtatg cataggaaac cagctgcttc
     7741 gtttgtgcgc ggtgggatcc atcggctttg catccgcgaa gcacccccgc aatcatgatt
     7801 caaccccctc tatttgaaat gtcggtgtcg cacttgcacc tgcattcgcc tcacagccac
     7861 ggccacggcc gagaagccga gaagccgaga atccgagaat ccgagaatcc gagaagccga
     7921 gaagccgaga agccgagaag ccgagaagcc gagaagccga gaagccaaga agccgagaag
     7981 ccaagaagcc aagaagccaa gaagccaaga agccaagaag ccaaaaccag agaagccaaa
     8041 aaaccaaaaa gcagagaagc agagaaacgg agaagcggag aagccgcaat cgctgctgca
     8101 ttgactatgc gatgacgagc catggtacgg ggtaaaacgc gagagcgaag acgtggaggt
     8161 tggtgttatg aaatactcta cggcgcgcgc agcgccgccg gaagaatgac ggctttgtca
     8221 ctcaggccga atgtgcgagg gcacggattc cagatgcacc gctaccgtca ctgtgcgggg
     8281 gacccgctta agagctggcg gggtgagccg ctttcttttg cctacttttc tttgcggccg
     8341 gcaaagaaaa gtaggtgcct gccccgcaca ggggcgacgc taatagacca ataagaaaac
     8401 aaggaaaggc caacgccata aaaagcacag acaaaaagca cagacaaaag gcgcctggtg
     8461 tgtatcggcg cgttggagcg ccgcaagcga ggcgtggcgc aaggcgttgc cgaagtggcc
     8521 caggatcggt tgcctgacca tcgggtgcct gttgttcgta agccccgaac gagggcaacg
     8581 ccaatcaacc aatagcgaaa cggggatagg caccgccaaa gcaaacattt agtcagcacg
     8641 cagccaggca taaacccgct gtttgaaggt taagaacatc ctgaatcgcc caaagcatcc
     8701 taatatcgag ccgaaccgaa aaaaacgacg ggggaagcca tcatgtatcg tcatcgttcg
     8761 ctaggtgtcg tgacaggcgc gtcgccgctc gccggcgtcg acgtgttgag caggatgacc
     8821 gcggcgtggc gccggcccgg cgccgccagg ccattcgatt tcgtcctcga gcagcagtct
     8881 tggcagggac cggcttcgct aagcggggcg agcgcaccat ttaaaattca cgtgttcgac
     8941 acgattcgcg ccttcgagaa acgcggggtc gacgcgatcg tcttaccgtg ctttctcagt
     9001 cacaccttca tcgacgaact ctcggcgaac gtgacgctgc cgatcgccaa tctcatgacc
     9061 gcgctcgtgg cgcatctgcg gcaggctttt ccatcgcttc gccgcatcgg cgtgctgact
     9121 tccgacacga tccgcagtaa tggtcttttc gaacgctatt tcgatagcgc ggggttcgac
     9181 gtgctgcatc cgcgtaatga ggcgggtgtc gatcgcgtaa cgagcgccgt gtacggcgat
     9241 gaggggatca aagggggcca tctatacggc cggccagtcg agttgctgcg tgc
//
LOCUS       DATASH010000465         1764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6203961,
            whole genome shotgun sequence.
ACCESSION   DATASH010000465 DATASH010000000
VERSION     DATASH010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6203961"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /gene="panB"
                     /locus_tag="VHV99_12870"
     CDS             <1..727
                     /gene="panB"
                     /locus_tag="VHV99_12870"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX3636363.1"
                     /translation="TCYDASFAALLDRAGVDVLLIGDSLGNVLQGQTTTLPVSLTEIA
                     YHTASVARARPSALVVADLPFGTYGTPEDAFRSSVELMRAGAQMVKLEGGEWLADTVR
                     FLVERSIPVCAHVGLTPQSVHAFGGFKVQGKTDAGASQVLRDSLAVQNAGAQLIVLEA
                     IPTLLGTEVTKQLQIPTIGIGAGLDCSGQVLVLHDMLGIFPGKRPRFVKDFMQGQPNI
                     QAAVEAYVRAVKDGSFPGPEHTF"
     gene            complement(887..>1764)
                     /locus_tag="VHV99_12875"
     CDS             complement(887..>1764)
                     /locus_tag="VHV99_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEX3636364.1"
                     /translation="TATATATAAPAASHTTCGDFCLSVAIRTLTLAPAVQPGGEWQGK
                     MGVGAGIVLDSVAADEYAECQLKASFLTGAEPGFQLFETMYATREEGVRHLSRHLARL
                     SASAATLGFKLDGETEIRAQIAEKCAALPAQSPHRMRLALSKNGTAQITAAVLTPLVD
                     STVGVLLGPDHGFRATDANDPLMRHKTTRRAEYDRGWREAEAKSAFDTLFFNERGELT
                     EGGRSNVFVKLEGRWWTPPLASGVLPGVMRGALLDDTDLHAAERVLTRADVQKAEALL
                     VCNALRGAVQARVMD"
BASE COUNT          291 a          554 c          584 g          335 t
ORIGIN      
        1 gacctgttac gacgcgagct tcgccgcgct actggatcgc gcgggtgtcg acgtgttgct
       61 gatcggcgac tcgctcggca acgtgctgca aggccagacg accacgctgc cggtgtcgct
      121 cacggagatc gcgtatcaca ccgcgagcgt cgcgcgggcg cggccttcgg cgctggtggt
      181 ggccgacctg ccgttcggca cgtacggcac gccggaggac gccttcagga gctcggtgga
      241 actcatgcgt gccggcgcac agatggtgaa gctcgagggt ggtgagtggc tcgctgacac
      301 ggtgcgcttt ctggtcgagc ggtcgattcc ggtgtgcgcg cacgtcggtc tgacgccgca
      361 atcggtgcat gcgtttggcg gcttcaaggt gcagggcaag accgacgccg gcgcgagtca
      421 ggtattgcgc gactcgctcg ccgtgcagaa tgcgggcgcc caactgatcg tgctcgaagc
      481 gattccaacg ctgctcggga cggaagtgac gaagcaattg cagattccga cgatcggtat
      541 cggcgcgggc ctcgactgct cgggccaggt gctggtgctg cacgatatgc tggggatttt
      601 ccccggcaag cggccgcgct tcgtcaagga tttcatgcaa gggcagccga acatccaggc
      661 tgccgttgaa gcgtatgtac gagcggtgaa ggacggctcg ttccctgggc cggagcatac
      721 gttctgaagt tctgaacaca cgatctgata gccgacagca tgccatgcgg gattgcacat
      781 ggcatgcagg cctgacgggg tggatcgccc gcttactgca cgttcggcat cgttgagccg
      841 tcgcagggca cgtgcggcgc aaaggctaca gcgcgtacgc tcgacctcaa tccatcaccc
      901 gtgcctgcac cgcgccgcgt agtgcattgc acaccaggag cgcttcggcc ttctgcacat
      961 cggctcgcgt cagcacgcgc tcggcagcgt gaaggtcagt gtcatcgagc agcgcacccc
     1021 gcatgacccc gggcagcacg cccgacgcta gaggcggcgt ccaccatcgg ccctcgagct
     1081 tcacaaacac attcgaccga ccgccctcgg tcaactcgcc gcgctcgtta aagaacagcg
     1141 tgtcgaaagc actcttcgct tccgcttcgc gccagccgcg atcgtattcg gcgcggcgcg
     1201 tggttttgtg ccgcatcaat ggatcattgg catccgtcgc gcggaaaccg tggtccggtc
     1261 cgagcaggac accgaccgtt gagtcaacca gcggcgtcag cactgccgcg gttatttgcg
     1321 ccgtgccgtt tttgctcaac gcgaggcgca tacgatgcgg gctttgcgct ggcaacgccg
     1381 cacatttctc tgcgatttgc gcgcgaattt cagtttcgcc gtcgagtttg aaaccgagcg
     1441 tcgcggcgct ggctgagagt cgtgcgagat ggcgcgacag atgccgcacg ccctcttccc
     1501 gcgtcgcata catcgtttcg aagagctgga aacccggctc ggcgccggtc agaaagctgg
     1561 ccttcagttg gcactccgca tattcgtcag cggcgacgct gtcgagcacg atgcccgcgc
     1621 cgacgcccat cttgccttgc cattcgccgc ctggctgcac ggctggcgct aaggtcaatg
     1681 tgcggatcgc gaccgacagg cagaagtcgc cgcaggttgt gtggcttgct gcgggtgcgg
     1741 ctgtggctgt ggctgtggct gtgg
//
LOCUS       DATASH010000466         2251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1656750,
            whole genome shotgun sequence.
ACCESSION   DATASH010000466 DATASH010000000
VERSION     DATASH010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1656750"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..107
                     /locus_tag="VHV99_12880"
     CDS             <1..107
                     /locus_tag="VHV99_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HEX3636365.1"
                     /translation="EGLIDGVWKVLDPTSIDLPPVDLAIQGRRGPLRP"
     gene            394..1455
                     /locus_tag="VHV99_12885"
     CDS             394..1455
                     /locus_tag="VHV99_12885"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEX3636366.1"
                     /translation="MAGSHFPAARLRPVFSSLYPLVRAQLFRMDAEDAHHLTLRMLGA
                     AGRAGLAGALGPRVPDAPRTVMGLTFRNPVGLAAGLDKDGAAIDGLAALGFGFIEVGT
                     VTPRAQPGNPRPRMFRLPQANAVINRMGFNNAGVDQFVKNVQAARYRGILGLNIGKNA
                     DTPIERAAEDYLYCLERVYPFASYVTVNISSPNTKNLRQLQGAGELDALLAALKDKQQ
                     HLADMHGKLVPLALKIAPDLDDEQIKSIADTLLRHHFEGVIATNTTLSRTAVTGMQYG
                     DEAGGLSGKPVFDASNEVIRKLRAEVGEAVPIIGVGGIFSGEDARAKLAAGASLVQLY
                     TGFIYRGPALVAECAQALR"
     gene            complement(1588..>2251)
                     /gene="lepB"
                     /locus_tag="VHV99_12890"
     CDS             complement(1588..>2251)
                     /gene="lepB"
                     /locus_tag="VHV99_12890"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX3636367.1"
                     /translation="SLVAFLFLMVLFRSAVADWNVVPSGSMLPTIREGDRIFVDKMAY
                     DLRVPLTHITIAHLHDPQRGDIVTIDSSAAHELIVKRLIGLPGDSVAMRDNVLYVNGV
                     RADYQPLHFKPLPGDATSPGDYLTERFDGVVHTVRLAPEAPSPRNSFGPVVVPPGEYL
                     MLGDNRDDSADSRYFGFFPRSELMGRTRHVAYSLDPDHHYKPRFERFGAPLDAASLAA
                     SR"
BASE COUNT          398 a          751 c          729 g          373 t
ORIGIN      
        1 tcgaaggttt gatcgatggt gtctggaagg tgctcgatcc aaccagcatc gatctgccgc
       61 cggtcgatct ggcgattcag ggcaggcgcg ggccgctgcg gccgtaaggt ccgcgggctt
      121 taccggccag gacggtacgg ccgacgcacc ccaacgccga cagcgtctgg atctcagcga
      181 ctgcttcggt cgaagtgcgg cgccctcgga catctttttg ggttgcgctg ccagcgctgc
      241 gccggcacac gaggccacgt cttcgtgttg cgctgccacc gccgcgccgg cacacgaggc
      301 cacctcacgc accctcaccg gcagcactca ctccgacacc cgcgccttcg cttccctttc
      361 agttagtctc attaaagcct caaagccggt aaaatggcgg gttcccattt tccggccgcc
      421 cggcttcgtc ctgtgttcag ctccctctac ccgctcgtac gcgcccaact ctttcgcatg
      481 gatgcggaag acgctcacca cctgacattg cgcatgctcg gcgccgccgg ccgcgccggt
      541 ctcgccggcg cgctcgggcc acgcgtgccg gatgcgccgc gcaccgtgat ggggctgacg
      601 ttccgcaatc cggtcgggct cgccgcaggc ctcgacaagg acggcgccgc gatcgacggc
      661 ctggccgcgc tgggcttcgg cttcatcgaa gtgggtacgg tcacgccgcg cgcgcagccg
      721 ggcaacccgc gaccacgcat gttccgcctg ccgcaggcga atgccgtgat caaccggatg
      781 ggcttcaaca acgcgggcgt cgaccagttc gtcaagaacg tgcaagccgc gcgctatcgc
      841 ggcattctcg ggctgaatat cggcaagaac gccgacacac ccatcgagcg cgccgccgaa
      901 gactatctgt actgcctcga acgcgtgtac ccgttcgcga gctatgtgac ggtcaacatc
      961 tcgtcgccga atacgaagaa tctgcgccag ttgcaaggcg ccggtgaact cgacgcgctg
     1021 ctcgccgcgc tgaaggacaa gcagcagcat ctggccgaca tgcacggcaa gctggtgccg
     1081 ctcgcgctga agatcgcgcc ggacctcgac gacgaacaaa tcaaatcgat cgccgatacg
     1141 ctgttgcgcc accacttcga aggcgtgatc gccaccaata ccacactgtc gcgcaccgcc
     1201 gtcaccggca tgcagtacgg cgacgaggcc ggcggcctgt cgggcaagcc ggtgttcgac
     1261 gcgtcgaacg aagtgatccg caaactgcgc gcggaagtcg gcgaggccgt gccgattatc
     1321 ggcgtgggcg ggattttctc gggcgaggat gcgcgcgcca agctcgcggc gggcgcgtca
     1381 ctggtgcagc tctataccgg cttcatctac cgcggaccgg cactggtggc cgaatgcgcg
     1441 caggcgctgc gctgaagctg acgccaggta gtgggccttc gggctcactg ggccttcggg
     1501 cgtagtgggc cttgcgggcg tagtgtgtct tcgggcgtag tgggccttgc cggtacagtg
     1561 cgtcttccgg cgcagaagct tcgccgccta acggctggcg gctagcgacg ccgcatccag
     1621 cggcgcgccg aagcgctcga agcgcggttt gtagtggtgg tccgggtcga gcgaatacgc
     1681 gacgtgacgc gtacggccca tcaattcgct gcgcggaaag aagccaaagt aacgcgagtc
     1741 ggcgctatcg tcgcggttgt caccgagcat cagatactcg ccgggcggca ccaccaccgg
     1801 tccgaacgaa ttgcgcggac tcggcgcctc gggcgcaagg cgcaccgtat gcacgacgcc
     1861 gtcaaaacgc tcggtcagat agtcgccggg ggacgtagca tcgcccggca acggcttgaa
     1921 gtgcagcggc tgataatccg cgcgcacgcc gttcacgtac agcacgttgt cgcgcatggc
     1981 gacactgtcg ccgggcaagc cgatcagacg cttgacgatc aactcatggg ccgccgaaga
     2041 atcgatcgtg acgatatcgc cgcgctgcgg atcgtgcaga tgcgcgatcg tgatgtgggt
     2101 gagcggcacg cgcagatcgt aggccatctt gtcgacgaag atccggtcgc cttcgcggat
     2161 cgtcggcaac atcgagccgc tcggcacgac gttccagtcg gccacggcgc tgcgaaacag
     2221 caccatcagg aacagaaagg cgacaaggct c
//
LOCUS       DATASH010000467         5978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3435220,
            whole genome shotgun sequence.
ACCESSION   DATASH010000467 DATASH010000000
VERSION     DATASH010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5978
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3435220"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="VHV99_12895"
     CDS             complement(<1..581)
                     /locus_tag="VHV99_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008346927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEX3636368.1"
                     /translation="MNYELLFRAGAHAAMRAGKGISAAATPEVRYALTGAKGGFARTL
                     LAQTRLMSRLAPTLLCDLDVAGLRALCEELGYAAADLVECSTPDEVAALPQNAIGLIA
                     DVALLDPARYNILVEATGNPSIGYRCARRALQHKRHVAMVSKEVDAVAGVSLAQLAAR
                     EGVVYTTADGDQPSNLIGWVSWARTLGLEVVAAG"
     gene            791..2128
                     /gene="otnK"
                     /locus_tag="VHV99_12900"
     CDS             791..2128
                     /gene="otnK"
                     /locus_tag="VHV99_12900"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HEX3636369.1"
                     /translation="MTSTSRPLLGCIADDFTGATDLANMLVRGGMRTVQTIGVPAAAT
                     LIEADALVVALKSRTISAADAVAQSLAALAWLRAQGCRQFFFKYCSTFDSTEAGNIGP
                     VTDALLDTLSAAPNSAPASHNFTIACPAFPENHRTVYRGHLFVGDALLSESGMENHPL
                     TPMTDANLVRVLQRQTRSKVGLLRYDTIAQGAQAVRNAIEQMRADGTRIAIADAVSDA
                     DLHTLGAACAELALITGGSGVALGLPANFQASGSLRASHAADALPAVAGPSVVLAGSA
                     SKATNAQVAAWLATRPGFRIDPLALARGEPVVEQAVAFAQDRATSGGSQPVLIYATTT
                     PDEVKTVQRELGVARAGQLVEDALAAIARRLREAGVRKFVVAGGETSGAVVQALEVRM
                     LRIGAQIDPGVPATASVPSHPDEAPLALALKSGNFGAVDFFDKALARLNGGLQ"
     gene            2125..2787
                     /locus_tag="VHV99_12905"
     CDS             2125..2787
                     /locus_tag="VHV99_12905"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014251447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HEX3636370.1"
                     /translation="MTGTMLNVSSTETRLREEICVVGASLYERGYTVGSAGNISARLD
                     DGWLITPTDACLGRLDPAGIAKVDLSGQPVSGGQPSKTLALHRGIYANNTETRGIVHT
                     HSTHLVALTLAGVWRATDVLPPITPYYVMKVGHVPLIGYRRPGDPQVAEQIAALAAQV
                     RAVLLERLGPVAWERSVSHASYVLEELEETARLWLMSAPRPEPLDDTAIEELKAVFRA
                     RW"
     gene            complement(2853..3734)
                     /locus_tag="VHV99_12910"
     CDS             complement(2853..3734)
                     /locus_tag="VHV99_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX3636371.1"
                     /translation="MNPANPERKTPPNELGALLRHWRDTRGVSQLDLSFNAGVSQRHI
                     SFIESGRSVPSRQMLMDIAQTLDIPLRERNTLLLAAGYAPMYADSAWNAQEMQSVTKA
                     LGRMLRQHEPFPALVMDRYWNVLMTNESAPRFFNSFIDMAARKGPRNMLHLIFDPDGM
                     RPFVADWQTVANSLIQRVYRESVGRVVDARTHELLDALRAYPDVHADLKSSNSTSEEA
                     GSATAAMPVIPVGFVKDGQVLNYFSMVATVGTPQSVAAQELRIECMFPADDETEALHL
                     QMIEQATEQTDSGRHHA"
     gene            complement(3880..4428)
                     /locus_tag="VHV99_12915"
     CDS             complement(3880..4428)
                     /locus_tag="VHV99_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX3636372.1"
                     /translation="MSTFHLHTIESAPEQSRPVLRQLQQTFGLIPNIAGAMAESPVLI
                     GAFIGLFQKVHAGTFTEAQIQTLLLTNAVTNACSWAVAFHTALALNEGLSKTDVDAIR
                     NGRAPDDRKHAALSSLTKTAIEKRGHLDDKDISQFLEAGFRHDQVLEVLAVLAASTIT
                     NYAGNITRPAVEAPFQAHLWNA"
     gene            complement(4542..5312)
                     /locus_tag="VHV99_12920"
     CDS             complement(4542..5312)
                     /locus_tag="VHV99_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3636373.1"
                     /translation="MAEVVEALRGEDIVKRFGAVTALDGVSLTLRQGEILGVLGDNGA
                     GKSTLIKILTGFHQQTSGKLFVGGEETLLRSVDHARSLGIECVYQDLALANSLSIYHN
                     MFLNREIIRRGPFRLLNNRAMRTRAAECLEEIGVHVPSVDLAVEQLSGGQRQAIAVAR
                     AVNSNARILLLDEPLAAMGAREAALIIDLILRLKEKGGLSIVMIMHNYAQTLDIADRV
                     MLMQRGRVTYQREAASTSVEELMDIVRREYRAMRTAPH"
     gene            complement(5312..>5978)
                     /locus_tag="VHV99_12925"
     CDS             complement(5312..>5978)
                     /locus_tag="VHV99_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3636374.1"
                     /translation="GVLAASLVGLINGAVTVYLRIPSFVTTLGTLFFVNGLTLTISRG
                     TPVSPPENSAFAAFMGEWGYSEIIWTLAIAAFMHVLLRHTRWGLHTIASGANPLGASE
                     AGIQVRRLKLGNFILAALLAGLTGILEGFRITSIDPQAGGNQIMFLAVAAAVIGGTPL
                     AGGSGTIIGGLIGAAVLGILNDGFTLIGINAFTFNMILGAAILGAMIFNIHVVRLARK
                     GEL"
BASE COUNT         1115 a         1907 c         1874 g         1082 t
ORIGIN      
        1 ccggccgcga cgacctcgag gccaagcgtg cgcgcccagc tcacccaacc gatcagattc
       61 gacggctggt cgccgtcggc ggtcgtgtag accacgcctt cacgcgccgc gagttgcgcg
      121 agcgagacgc cggccactgc atccacttcc ttgctgacca tcgccacgtg acgcttgtgc
      181 tgcaacgcgc ggcgtgcgca gcgatagccg atcgacggat tgccggtggc ttccacgaga
      241 atgttgtagc gcgccgggtc gagcaacgcg acgtccgcga tcagcccgat ggcgttctgc
      301 ggcagtgcgg cgacctcgtc cggcgtgctg cattcgacca gatccgcagc cgcgtaacca
      361 agctcctcgc acaatgcccg caggcccgcg acgtccagat cgcacagcag cgtcggcgca
      421 agccgtgaca tcagacgcgt ttgcgcgagc agcgtgcgag cgaagccgcc cttcgcgccg
      481 gtcagcgcgt agcgaacctc cggcgttgcg gcggccgata tgccctttcc ggcgcgcatg
      541 gcggcgtggg cgccggcgcg aaacagcaac tcgtagttca tttcaagtcc cctgcaatga
      601 ctgcgcgccg atgcgcgccg cgtccgtatc gcggcgattc gcctggcact gaataggcag
      661 atagggagca tcgacgctgc cactccccag cagcggccac cttaatgaga tttaccattt
      721 tagtcaaatt ctttaacacc gattcacatg caacaggttg atggctacaa tcgaggcaaa
      781 ggagatatcc atgacttcga cctcacgccc gttgctgggc tgcatcgccg acgacttcac
      841 cggcgcgacg gatctcgcga acatgctggt tcgcggcggc atgcgaaccg tccagaccat
      901 cggcgtgccc gccgccgcca cgctcatcga ggccgatgcg ctggtggtcg cgctcaaatc
      961 ccgcacgatt agcgccgccg acgccgtggc gcaatcgctt gccgcgctgg catggttgcg
     1021 cgcgcaaggg tgccggcaat tcttcttcaa atactgctcg accttcgact ccaccgaggc
     1081 cggcaacatc ggcccggtga ccgatgcgct gctcgacaca ctgagcgccg cgccgaactc
     1141 agcccccgct tcacacaact tcacaatcgc ctgccccgcg tttcccgaga atcaccgcac
     1201 cgtgtatcgc ggccacctgt tcgtcggcga cgcgctgttg agcgagtcgg gcatggaaaa
     1261 tcatccgctt acgccaatga ccgacgcgaa cctcgtgcgg gtgttgcagc gccagacccg
     1321 ttcgaaggtc ggcctgttgc gctacgacac gattgcgcaa ggtgcgcagg cggtgcgcaa
     1381 cgccatcgaa cagatgcgcg ccgacggcac gcggatcgcg atcgcggatg ccgtgagcga
     1441 tgccgatctg catacgctcg gcgcggcgtg cgccgagctt gcgttgatta ccggcggctc
     1501 cggtgtcgcg ttgggtttgc cggccaactt ccaggcgtcc ggctcactgc gcgcgagcca
     1561 cgcggccgat gcgttaccgg ccgtcgcggg cccgtcggtc gtgctggccg gcagtgcctc
     1621 gaaggccacc aacgctcagg tggcggcatg gttggccacg cgccccggtt tcaggatcga
     1681 tccgctcgcg ctggcacgcg gtgagccggt agtcgagcag gcggtggctt ttgcccagga
     1741 ccgcgcaaca tccggcggct cccagcccgt gctgatctac gcgaccacca cgccggacga
     1801 agtgaagacc gtgcaacgcg agctgggtgt ggcgcgcgcc gggcagctcg tggaagacgc
     1861 gctggcggca atcgccaggc gattgcgcga agcgggcgtg cgcaaattcg tggtggcggg
     1921 gggtgagaca tcgggcgcgg tggtccaggc gctcgaggtg cggatgctgc gcattggcgc
     1981 gcaaatcgat ccgggcgtgc cggccacggc gagcgttccc tcccatccgg acgaagcacc
     2041 actggccctc gccctgaagt cgggcaattt cggcgcggtc gacttcttcg acaaagccct
     2101 tgcgcgactg aatggaggcc tccaatgacg ggcacgatgc tcaatgtgag cagtacggag
     2161 acacgtctgc gagaagaaat ctgtgtggtc ggcgcgagtc tgtacgagcg tggctacacg
     2221 gtaggcagcg cgggcaacat cagcgcccgc cttgatgacg gctggctgat cacgcccacc
     2281 gacgcgtgtc tcggccggct cgatccggcc ggcatcgcca aggtggatct gagcggccag
     2341 ccggtgtcag gcggccaacc ctccaagaca ctcgcacttc atcgcggcat ctacgccaac
     2401 aataccgaaa cgcgcggcat cgttcacacc cactccacgc acctggtggc gttgacgctg
     2461 gccggtgtct ggcgcgcaac cgacgtactg ccgccgatca cgccttacta cgtgatgaaa
     2521 gtgggtcacg taccgctgat cggctatcgg cgcccgggcg atcctcaggt ggccgaacag
     2581 atcgccgcgc tggcggcgca agtgcgcgcc gtgctgctcg aacgacttgg accggtggca
     2641 tgggagcgtt ctgtctcgca cgcgtcgtat gtgcttgaag aactggaaga gacggcgcgt
     2701 ctgtggttga tgtcggcgcc acggccggaa cctctggacg atacggcgat tgaagagttg
     2761 aaggccgtgt ttcgggcgcg ctggtgacgc tcgaagcaga gcgcgggggc ggagcggcga
     2821 gcacggcggc aagcgcagcg ccgggtccgg attcaagcgt ggtgacgccc ggaatcggtc
     2881 tgctcagtcg cttgctcgat catctgtaga tgaagtgcct cggtttcatc gtccgcgggg
     2941 aacatgcatt cgatccgcag ttcctgtgcg gcgaccgatt gcggcgtgcc cacggtagcg
     3001 accattgaaa agtagttcag gacctggccg tccttgacga agccgacggg tatcaccggc
     3061 atagctgctg tagcggaccc ggcttcctcc gaagtactgt tggaagactt caaatcagcg
     3121 tgcacatccg gataagcacg cagcgcatcg agcaactcgt gcgtccgagc gtcgaccacg
     3181 cgaccgaccg actcccgata aacacgctga atcaggctat tcgccaccgt ttgccaatcg
     3241 gcaacgaacg gacgcatccc gtccggatcg aaaatcagat gcagcatgtt gcgcggtccc
     3301 ttgcgcgccg ccatatcgat gaagctgttg aaaaaacgcg gcgcggattc gttggtcatc
     3361 agcacgttcc agtagcggtc catcacgagc gctggaaacg gttcgtgctg acgcaacatt
     3421 cgcccgagtg ctttcgtgac gctctgcatt tcctgggcgt tccaggcgct gtcggcatac
     3481 atcggcgcgt accccgctgc cagtagcagc gtattgcgtt cgcgcagagg aatgtcgagc
     3541 gtctgggcga tatccatcag catctgacgg ctcggcacgc tgcgtccgct ttcgataaaa
     3601 ctgatgtgcc gctgcgagac acccgcgttg aacgacaagt ccaattggct gacgccgcgc
     3661 gtgtctcgcc agtgacgcaa caacgcgccg agttcattcg gcggcgtctt tctttcgggg
     3721 ttcgcgggat tcatcggtct cctgtccgca ggttgaatat agatagaagg cttgatagcc
     3781 ggtggcgcgg aaagtcgtgg acgtccagcg ccgaccgctc gctcgaaccg gaccggtcag
     3841 catactcctt gctgccgggc ggcccgtttc aacggatcgc tacgcgttcc agagatgcgc
     3901 ctgaaacggc gcttccaccg caggccgggt gatgttgccg gcgtagttcg tgatcgtcga
     3961 ggcggccagc accgcgagaa cctcgagcac ctggtcgtga cggaacccgg cttcgagaaa
     4021 ctggctgatg tccttgtcat ccaggtgtcc gcgtttttcg atcgctgttt tcgtcaagct
     4081 ggagagcgcc gcatgcttgc gatcgtccgg cgcgcggccg ttgcgaatgg cgtcgacgtc
     4141 ggtcttcgaa aggccttcgt tcaacgcaag ggcggtgtga aacgcaaccg cccaggaaca
     4201 cgcgttcgtc actgcgttag tcagcagcag agtctgtatc tgtgcttccg tgaaagtgcc
     4261 tgcatgaact ttctggaaca ggccaataaa ggcgccgatc aacacgggcg attcagccat
     4321 ggctcccgca atgttaggaa tcaggccgaa ggtttgctgc aattgccgga gcaccggcct
     4381 ggattgttca ggcgcggatt caatcgtatg aaggtgaaac gtcgacatga gctgtcctcg
     4441 atcgtgagcg ccactcggga tgagttgcgc gatgaccgaa gcttaggcgt gagcgtgcgg
     4501 ctcgccaatt acctctcatg taataggatt tttaccgcca atcagtgcgg cgccgtccgc
     4561 attgcccgat attcgcgtcg cacgatatcc atcaactcct caaccgacgt gctggcggcc
     4621 tcccgctgat aagtcacccg tccacgctgc atcagcatca cacgatcggc gatgtcgagc
     4681 gtctgggcgt agttgtgcat gatcatcacg atagacagtc cgcctttctc cttgagccga
     4741 aggatcaggt cgatgatcag cgcggcctca cgggcgccca tggcggcgag cggctcgtcg
     4801 agcaggagga tccgtgcatt cgaattgacg gcacgagcga ccgcgatagc ctgccgctgc
     4861 ccgccggaaa gctgctcgac cgccaggtct accgaaggca cgtgcacgcc gatttcctcg
     4921 agacactccg cggcgcgagt acgcatcgcg cggttgttca acaaccggaa cggcccccgg
     4981 cggatgattt cccggttcag gaacatgttg tgataaatac tcagcgaatt ggccagcgcc
     5041 agatcctggt agacgcattc aatgccgagc gagcgggcgt gatcgacgga gcgaagcagc
     5101 gtttcctcgc cgccgacaaa aagcttgccg ctagtctgct gatggaaccc ggtgagaatc
     5161 ttgatcagcg tcgacttgcc cgcgccattg tcgcccagca cgccgagaat ttcgccctgc
     5221 ctgagcgtca gcgacacgcc atcgagcgcg gtcacggcgc cgaaacgctt gacaatatct
     5281 tcgccgcgca gcgcctccac cacctcggcc atcacagctc tcccttgcgc gcgagacgaa
     5341 ccacgtggat gttgaagatc atggccccga gaatggccgc gccgagaatc atgttgaagg
     5401 tgaaggcgtt gatgccgatc agcgtgaagc cgtcgttgag aatgccaagc accgccgctc
     5461 cgatcagccc gcctatgatc gtgccggacc caccggcgag gggcgtgccg ccaatcacgg
     5521 ctgcggcaac tgcaagaaac atgatctggt tgccgcctgc ttgcggatcg atcgacgtga
     5581 tgcggaaacc ctccaggatc ccggtcaagc cggcgagcag cgcggcgaga atgaagttgc
     5641 cgagcttcag gcgtctgacc tgtatccccg cctcgctcgc gcccagcgga ttcgctcctg
     5701 acgcgatcgt atgcaagccc cagcgcgtgt ggcgtagcag cacgtgcatg aatgccgcaa
     5761 tggcaagcgt ccagatgatt tcgctgtaac cccattcccc catgaatgcc gcaaacgctg
     5821 aattttccgg tggtgaaacc ggcgtacctc gtgagatcgt cagcgtgagg ccgtttacga
     5881 agaacaacgt gccgagcgtc gtcacgaacg atggaatgcg aaggtacacc gtcaccgcgc
     5941 cgttgatcag cccgacgagg ctcgcggcaa ggacgccg
//
LOCUS       DATASH010000468         3077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6601437,
            whole genome shotgun sequence.
ACCESSION   DATASH010000468 DATASH010000000
VERSION     DATASH010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6601437"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VHV99_12930"
     CDS             complement(<1..192)
                     /locus_tag="VHV99_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636375.1"
                     /translation="MRLCIKKPPGRQLGLSARWLDKARSGERFLHQSDRACLATRPSA
                     NIKNSKRLAELIENRGVIER"
     gene            191..598
                     /locus_tag="VHV99_12935"
     CDS             191..598
                     /locus_tag="VHV99_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4212 domain-containing protein"
                     /protein_id="HEX3636376.1"
                     /translation="MAAPHHTSHATLNPAPEPPAVSAAMARAHQKYWRYNLALIATLM
                     TVGFVVSFVLPLVAPALEQVRFGGFRLPFYFGAQGAILIYVALILVYIVLMQRADRRL
                     QSTFEADASAGRAGAGASASASADSTAPTARGN"
     gene            603..2621
                     /locus_tag="VHV99_12940"
     CDS             603..2621
                     /locus_tag="VHV99_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEX3636377.1"
                     /translation="MTLTNRLIRSYALYMLGFLLFIYVMWRIERTTGPGVWIGYVFLF
                     VPIAVYAVIGLLSRTSDLVEYYVAGRRVPSAFNGMATAADWLSAASFIGLAGSIYATG
                     YDGLAYLMGWTGGYCLVAFLLAPYVRKLARYTIPDFLGTRFSSNAVRGLAALAAILCS
                     FVYLVAQIQGVGLIATRFIGVDFAVGIFCGLAGILVCSFLGGMRAVTWTQVAQYVILI
                     AAILIPVSMIAHKDGLGWVPQFNYGRLMERVEVLEKQVRDAPLEQTVRDDYRRRAALM
                     QMRLDTLPQSFVDEKLRLTQEVADLRRHNGPLREIKERERALEQFPRDAAAAQIVWTQ
                     RRDEMLMRAAAPVPMHEPFPATSDEDRRTHERNFLSLLLCLSLGTASLPHILTRYNTT
                     TSVASARRSVGWTLFFVALFYLTVPVLAVLIKYEMLTNLVGHHFADLPQWLTQWRKVE
                     PSLISLADTNGDGIVRWSEIQMQPDMVVLAAPEIAGLPYVMSGLIAAGALAAALSTAD
                     GLLLTIANALSHDVYYHMVDPTASSQRRVTISKILLLGVALFASYVASLNTGNILFLV
                     GAAFSLAASSLFPVLVLGVFWKRTTRLGAVAGMMAGLMVCIYYIVSTYPFFTQMTGFA
                     GARWFGIEPISSGVFGVPAGFLVAIGVSLVDRKPDAYTRALVDYIRHP"
     gene            2695..2785
                     /locus_tag="VHV99_12945"
     tRNA            2695..2785
                     /locus_tag="VHV99_12945"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2729..2731,aa:Ser,seq:gga)
     gene            2888..>3077
                     /locus_tag="VHV99_12950"
     CDS             2888..>3077
                     /locus_tag="VHV99_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636378.1"
                     /translation="MPSYTVVTRVVLHEDDAHTKEHKPDGAEYDVLHEEMWKRGYRRF
                     FEGASGKLRKLPPGEYKIY"
BASE COUNT          498 a          894 c          994 g          691 t
ORIGIN      
        1 acgctcgata accccgcgat tctcgatcag ctcggcgagt cgctttgagt ttttgatgtt
       61 tgctgagggg cgtgttgcaa gacacgcccg atctgactga tgcaaaaacc gttcacctga
      121 acgagcctta tccagccacc gcgcggacaa tcccaattgc cggcccggtg gctttttgat
      181 acataggcgc atggccgcgc cgcaccacac ctctcacgct acgctcaacc ccgcgcccga
      241 accacctgcg gtctcggcag cgatggcgcg cgcgcatcaa aagtattggc gctacaatct
      301 cgcgttgatc gcgacgttga tgacggtggg cttcgtggtg tcgttcgttt tgccgctggt
      361 ggcgccggcg ttggagcagg tgcgctttgg cggcttcaga ttgccgttct acttcggtgc
      421 gcaaggggcg attctgatct atgtcgcgtt gatccttgtt tatatcgtgc tgatgcagcg
      481 cgccgatcgc cgtctgcaaa gtacttttga ggcggatgct agcgctggcc gcgccggtgc
      541 cggtgccagt gccagtgcca gtgccgattc caccgcaccc accgcgcgtg gaaactgagc
      601 cgatgacgct cacgaaccgg ctgatccgct catacgcgct ttatatgctc ggcttcctgc
      661 tattcatcta tgtgatgtgg cgcatcgagc ggaccaccgg ccccggcgtg tggatcggct
      721 atgtgttcct gttcgtgccg attgcggtgt atgcggtgat cggcttgctg tcgcgcacct
      781 ccgacctcgt cgagtattac gtagccgggc ggcgcgtgcc gtctgccttc aacggcatgg
      841 cgaccgcggc cgactggttg tccgcggcat cgttcatcgg tctcgccggg tcgatctatg
      901 cgaccggata tgacggtctc gcttacctga tgggttggac cggtggctac tgcctcgtcg
      961 cctttctgct ggcgccgtac gtgcgcaagc tcgcgcgcta cacgattccc gactttctcg
     1021 gcacgcgctt ttcgagcaac gccgtgcgtg ggcttgccgc attagccgca atcctgtgct
     1081 cgttcgtcta tctggtcgcg cagatccagg gcgtcggctt gattgcgacg cgttttatcg
     1141 gcgtggattt cgcggtcggc attttctgcg gactggcggg cattctcgtg tgctcgtttc
     1201 tgggcggcat gcgtgctgtg acgtggaccc aggtcgcgca atacgtcatc ctgatcgcgg
     1261 cgattctgat tcccgtgtcg atgatcgcgc ataaggatgg cctcggctgg gtgccgcaat
     1321 tcaactacgg ccgtttgatg gagcgcgtgg aggtgctcga aaagcaggtg cgcgacgcgc
     1381 cactcgagca gaccgtacgc gatgattacc gccggcgcgc cgcgctgatg cagatgcggc
     1441 tcgatacgtt gccgcaatcg ttcgtcgacg agaagctgcg cctcacccag gaagtggcgg
     1501 acttgcggcg tcataacggc ccgctgcgcg agatcaagga gcgggagagg gcgctggagc
     1561 agtttccacg cgatgccgcc gccgcccaga tcgtctggac ccaacgccgc gacgagatgc
     1621 tgatgcgcgc ggcggcaccg gtgccgatgc acgagccgtt tcccgccacg agtgatgaag
     1681 accggcgcac gcatgagcgc aatttcctgt cgttgctgct gtgcctttcg cttggcaccg
     1741 cgagcctgcc gcacatcctg acgcgttaca acacgacgac ctcggtggcg tccgcgcgac
     1801 gttcggtcgg ctggacgttg ttcttcgtcg cgctgttcta tctgacggtg ccggtgctgg
     1861 ccgtgctgat caagtatgag atgctgacga atctcgtcgg tcatcacttc gctgatttgc
     1921 cgcaatggct cacacagtgg cgcaaggtcg agccgagtct gatcagcctc gccgatacga
     1981 acggcgacgg aatcgtgcgc tggagcgaga ttcagatgca gccggatatg gtcgtgctcg
     2041 ccgcgcccga gatcgcgggg ctgccgtatg tgatgtccgg gctgattgcg gcgggcgcgc
     2101 tggccgccgc gctttcgact gcggatggct tgctgctgac catcgccaat gccttgtccc
     2161 acgatgtcta ctatcacatg gtcgatccga cggcctcgag ccagcggcgc gtgacgatct
     2221 cgaagattct gttgctgggg gtcgcgttgt tcgcctcgta tgtggcgtcg ctgaacaccg
     2281 ggaatattct gtttctggtg ggcgcggcgt tttcgctggc ggcttcgagc ctgtttccgg
     2341 tgttggtgct gggtgtcttc tggaagcgca cgacgcggct tggcgcggtg gcgggcatga
     2401 tggccggact gatggtgtgc atttactaca tcgtgtcgac gtatccgttc ttcacgcaga
     2461 tgacgggctt cgcgggcgcg cggtggttcg ggatcgagcc gatcagctcc ggcgtgttcg
     2521 gcgtgccggc ggggtttctg gtggcgatcg gggtgagtct ggtggaccgg aaaccggacg
     2581 cttatacaag ggcgctggtg gactacatcc ggcatccttg agtgggcatg tttcgcgggt
     2641 ttggagaggg gtgtggcgca gggcgcgaag tttgctataa ttcggctccc cgccggagag
     2701 atggatgagc ggtttaagtc gcacgcctgg aaagcgtgta taggttaata acctatccgg
     2761 ggttcgaatc cccgtctctc cgccaggaca ccaatgaata caggcctcta cggcctgttt
     2821 tcgtttctac ccgccaaaga acccgcctta agatagaacg tagtatttct gccacgggag
     2881 gcacgaaatg ccgagctaca cagtcgtcac ccgcgtggtg cttcacgaag atgacgcaca
     2941 tacgaaagag cacaagcctg acggcgcgga gtatgacgtt ctgcacgaag agatgtggaa
     3001 acgtggttac cgtcgtttct tcgaaggagc gagcggcaag ttgagaaagc ttccacccgg
     3061 cgagtacaag atctatc
//
LOCUS       DATASH010000469         9284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6996977,
            whole genome shotgun sequence.
ACCESSION   DATASH010000469 DATASH010000000
VERSION     DATASH010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 9284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9284
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6996977"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..800)
                     /locus_tag="VHV99_12955"
     CDS             complement(81..800)
                     /locus_tag="VHV99_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006407193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HEX3636379.1"
                     /translation="MRTPTSRRRRPHIAGGSPVWLFDLDNTLHHASHAIFPAINRGMT
                     QYIIDALQVDIDEANRLRTGYTQRYGAALLGLARHHPLDPNDFLKVVHTFPDLGSMVR
                     HERGVARLVAALPGRKIVLTNAPEAYARAVLAELRIERLFEQVIAIEHMRDRRHWRAK
                     PDHSMLRRAMRDARVSLKDVILVEDTRSHLKNYRRLGIRTVWITGHLPRKPHADGVPT
                     RLPCRGQTEMSGLGLFRNVRF"
     gene            complement(1055..1954)
                     /gene="argB"
                     /locus_tag="VHV99_12960"
     CDS             complement(1055..1954)
                     /gene="argB"
                     /locus_tag="VHV99_12960"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX3636380.1"
                     /translation="MSELPDLSQIAPTLKAEILAEALPYIRQYHGKTVVIKYGGNAMT
                     EERLKQGFARDVILLKLVGINPVIVHGGGPQIDQALKKIGKQGTFIQGMRVTDEETME
                     VVEWVLGGEVQQDIVTLINHFGGHAVGLTGKDGGLIHARKMQMPDRDNPGQYLDIGQV
                     GEVEAINPAVVKALQDDAFIPVISPIGFGEDGLSYNINADLVAGKLAVVLNAEKLVIM
                     TNIPGVMDKEGNLLTDLSAREIDGLFADGTISGGMLPKISSALDAAKSGVRSVHIIDG
                     RIEHSVLLEILTEQPFGTMIRSH"
     gene            2221..2445
                     /locus_tag="VHV99_12965"
     CDS             2221..2445
                     /locus_tag="VHV99_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006407190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich CWC family protein"
                     /protein_id="HEX3636381.1"
                     /translation="MKSSASRSQSSALCPRCGHAFDCAMHGVPFDCWCREMPAMPADR
                     LDPSGRCLCPECLAAEIARAVQPASGVGSL"
     gene            complement(2534..4171)
                     /locus_tag="VHV99_12970"
     CDS             complement(2534..4171)
                     /locus_tag="VHV99_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing
                     glycosyltransferase family protein"
                     /protein_id="HEX3636382.1"
                     /translation="MLPTSDSATPASSAFLTFLRHAAAARQNGSAEAEASWLDAAAQF
                     HALEDASIESLIVALLEQHRHNDAIELAAIIAQLDPHRALAHFRFGYALQMANRHGEA
                     IAAYRRALAIDPTLPQLRNNLAGALTLTGGDPNEQLALLESAVHDDPDHGDAWTNLTQ
                     ASRANLNLSRALEAGARAVQCTPHSPLAHNNYALALREAQRWAEAEHAERTACALAPN
                     DATMRSNLGMLLLMRGNYAHGWQAHEARWDGSLELGGNRPAMPAPTWQGEPLAGKTLL
                     VWGEQGMGDVLQFSRYIPLLAERVRREGGRLVWNSFPQMGALLARSLGAHVNDYSAGG
                     GAESLPPFDYEIPLLSLPLLFDTREETIPAATPYLRPDAAASASWQQRLAGEARLKVG
                     LTWTGSPGHQRNPFRRVGWERYAAHFGGMQDVAFYSLQPGAEADVAAAQAAGLPMSDF
                     TAEFATFDDTAAFISALDLVITVCTSVAHLSGALGQRTWVLLDVNPHWVWLLDRPDSP
                     WYPSATLYRQPRFREWNPALEAVARDLSALADRQRTA"
     gene            4300..5610
                     /locus_tag="VHV99_12975"
     CDS             4300..5610
                     /locus_tag="VHV99_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3636383.1"
                     /translation="MHRITNTGRVNLSNLFWLRSLAIIGQLMTIAFVQIFIGVHLPLP
                     AMLLVIALEVIFNGLTWWRVSQQRLESNLELFGQIWVDLGALSALLFLSGGTNNPFVS
                     LYLPSLAIAAAVLPWHLMAWLAAFAVACYAVLGFDSVPLNLDNPANLFDYYRAGMWVN
                     FMVSVGLIAWFVARMSRALRLRDAALGDAQQRLLHDERAVALGVQAATVAHEIGTPLS
                     TIAMLSEELRDAARTDKGLAPYSADLELLEQQMTLCTSALARLRSRASTTTTQQTVGE
                     WLESFGEQWRLRHPHVKFERIGVPPAGVSLDDTVAVSQILTILLDNAARASRDHVTLS
                     CRLSARGDQVVFEVCDAGPGIPAALRGSLGTMPVDSTQGGHGVGLYLAFSAAARLKGS
                     IELNDVSEIKPRGTRAVLRLPLAGRKLSGAGRQGTSPSNTEKQA"
     gene            5607..6149
                     /locus_tag="VHV99_12980"
     CDS             5607..6149
                     /locus_tag="VHV99_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017334600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3636384.1"
                     /translation="MSDMNFLVIDDDEVFSGILARGLTRRGYTVAEAHNAAEAIKLAN
                     QQKFGQITVDLHLGNDSGLTLVAPLRDLQPDARMLVLTGYASIATAVQAVKDGADNYL
                     AKPANVETILSALQSEASALQADEAIEHPTPLSVARLEWEHIQRVLAEHGGNISATAR
                     ALNMHRRTLQRKLAKRPVRQ"
     gene            complement(6363..7709)
                     /gene="hslU"
                     /locus_tag="VHV99_12985"
     CDS             complement(6363..7709)
                     /gene="hslU"
                     /locus_tag="VHV99_12985"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEX3636385.1"
                     /translation="MSTMTPAEIVSELDKHIIGQGRAKKAVAVALRNRWRRQQVEDPL
                     RQEITPKNILMIGPTGVGKTEIARRLAKLADAPFIKIEATKFTEVGYVGRDVDSIVRD
                     LIEISVKQTRETEMRKVRTKAEDQAEDRVLDILLPSARPVGFGASSSAADTVDEGSTT
                     RQTFRKRLREGLLDDKEIELDVEQPQVGMDIMGPPGMEDMTEQIRSMFANIGGGKKTR
                     RKLKVKEALKVLTDEEAGKMLNDEEVKAKAVQNVEQNGIVFLDEIDKIASRNEAGGGE
                     VSRQGVQRDLLPLVEGTTINTKYGMVKTDHILFIASGAFHLAKPSDLIPELQGRFPIR
                     VELDSLSVGDFESILVSTDASLVKQYQALLATEDVHLEFADDGIRRMAEIAYSVNEKT
                     ENIGARRLYTVIEKLLEDVSFAAGNHSGKTVQIDAAYVDRALNEVAEDEDLSRYVL"
     gene            complement(7719..8255)
                     /gene="hslV"
                     /locus_tag="VHV99_12990"
     CDS             complement(7719..8255)
                     /gene="hslV"
                     /locus_tag="VHV99_12990"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006760749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEX3636386.1"
                     /translation="MEQFHGTTIVSVRRGDKVALGGDGQVTLGNIVMKGGAKKVRRIY
                     NGKVLVGFAGGTADAFSLLDRFEAKLEKHQGNLTRAAVELAKDWRTDRMLRRLEAMLI
                     AADATTTLVITGNGDVLDPEGGICAIGSGGAYAQAAAKALTDNTEMSPREIVEKSLEI
                     AGDMCIYTNHNRVIETIE"
     gene            complement(8565..8984)
                     /gene="dksA"
                     /locus_tag="VHV99_12995"
     CDS             complement(8565..8984)
                     /gene="dksA"
                     /locus_tag="VHV99_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEX3636387.1"
                     /translation="MTTKRLLTEAEILKMSDKDYMNEDQLAFFKNKLEQLQADILRNA
                     GQTTENLRETVIVPDPADRATIEEEHALELRTRDRERKLLKKVQQSIARIDSGDYGWC
                     EETGEPIGIPRLLARPTATLSLEAQERRELRQKLFGD"
BASE COUNT         1662 a         2781 c         2965 g         1876 t
ORIGIN      
        1 gaatgcagcg atcttgagaa ctgccggaac gcgggcaggc tcggtgttta aaaccccttc
       61 cagatagaga tttctgaacc tcagaaccgg acatttctaa aaagtcccaa cccggacatt
      121 tcagtctggc ctcgacaagg cagacgcgtc ggcacgccgt ccgcatgcgg cttgcgcggc
      181 agatggccgg tgatccacac ggtacggatg ccgagccgcc ggtagttctt cagatgcgag
      241 cgcgtgtctt ccacgaggat cacgtccttg agggaaacgc gcgcgtcgcg catcgcccgg
      301 cgcaacatcg aatggtcggg tttggcgcgc cagtggcggc gatcgcgcat atgttcgatc
      361 gcaatcacct gttcgaacag ccgctcgatg cgcagctccg caagcaccgc gcgcgcatag
      421 gcctcgggcg cattggtcag cacgatcttg cgaccgggca gcgcggcaac cagacgcgcg
      481 acgccgcgtt cgtgacggac catcgaaccg agatcgggga acgtatggac gactttcagg
      541 aagtcgttcg ggtcgagcgg atggtggcgt gccagaccga gcagcgccgc gccgtagcgt
      601 tgcgtgtagc cggtgcgcag gcggttggct tcgtcgatat cgacttgcaa tgcgtcgatg
      661 atgtattgcg tcatcccccg attgatcgcc gggaagatcg cgtgcgacgc gtggtgcaac
      721 gtgttgtcga gatcgaacag ccagaccgga ctgccgcctg cgatgtgcgg acgacggcgc
      781 cgggaagtgg gagtgcgcat gatgaaagac gccgttcagg cgcgagcagt cgcatcacgc
      841 agcggacgcg gagggttcgc ggtgcggatg gcggtgaaag aggggagagc ttgctggcgc
      901 gagagggcgg cgcgatgtgg gtcaccgcgc tgtgggtcgc cgtggcgtga gcccgcgccg
      961 ggcatgcagg aacccgcgga gacagcggtg gtactgcacg gcacacatgg cacgccgggc
     1021 aagcgtgccg tatgtgccgg gtttgaggca ggaatcaatg cgagcggatc atcgtgccga
     1081 acggctgttc ggtgagaatt tccagcagca ccgagtgctc gatgcggccg tcgatgatgt
     1141 ggacggaacg cacgccgctc ttggctgcat ccagcgccga cgagattttc ggcagcatgc
     1201 cgccggagat cgtgccgtcc gcgaacaggc cgtcgatttc gcgcgctgac agatcggtca
     1261 gcagattgcc ttccttatcc atcacgcccg ggatgttggt catgatcacg agcttttcgg
     1321 cgttcagcac gaccgccagt ttgcccgcga ccagatccgc attgatgttg tacgacaggc
     1381 cgtcttcgcc gaagccgatc ggggagatca ccggaatgaa cgcgtcgtct tgcagcgcct
     1441 tcacgaccgc cggattgatc gcctcgactt cgcccacctg gccgatatcg aggtactggc
     1501 ccggattgtc gcgatccggc atctgcattt tgcgcgcgtg gatcaggccg ccgtctttgc
     1561 cggtcagacc gaccgcgtgg ccgccgaaat ggttgatcag tgtgacgata tcttgctgaa
     1621 cttcgccgcc gagcacccat tcgacgactt ccatcgtctc ttcgtcggtg acgcgcatgc
     1681 cctgaatgaa cgtgccctgt ttgccgattt tcttcagcgc ctggtcgatt tgcggaccgc
     1741 cgccgtgcac gatgaccgga ttgataccga ccagcttaag caaaatcacg tcgcgcgcga
     1801 agccttgctt cagccgctct tcagtcatgg cattgccgcc gtatttgatg acgacggtct
     1861 taccgtgata ctggcgaata tagggcagcg cctcggccag gatttcagcc ttcagggtgg
     1921 gcgcgatctg cgaaaggtca ggaagctcgg acatggcggc aggctcaggc acggaacagt
     1981 taaaacagcg cgaattgtac aggactggcg cgctgcaaca gggttttcga tcccggcgcc
     2041 gatggcggcc ggagcgggct tttgcgcact gcgcaatgga cgggcgcaag cgccggccga
     2101 caatcgagcc taccgccgat cggacaggcc ggtaaatcgg ccgaatggcc gactcacggc
     2161 ggcccgccag tcgtggcatc atttccgggt tcaggctgaa gcgagaccac ccggcccgtt
     2221 atgaaatcgt cagcttcccg atctcaaagt agcgcgcttt gtccgcgttg cggtcacgca
     2281 ttcgattgcg ccatgcatgg ggtgccgttt gactgctggt gccgtgagat gccggctatg
     2341 ccggcagacc ggctcgatcc gtccggccgt tgcctatgtc ctgaatgtct cgcggcggag
     2401 atcgcgcgag ccgtgcagcc ggcgtcggga gtcggttcgc tgtgaagtgt agaaacgcgg
     2461 aaacgtagaa acccggaaac ccggaaaccc aagcgccctg aacaccgtgc ggcttcaaca
     2521 atgcggtggc gatctatgcc gtgcgttgcc ggtccgcgag cgcgctcaaa tcacgtgcga
     2581 ctgcttccag cgccgggttc cattccctga accgtggctg ccggtagagc gtcgcgcttg
     2641 gataccacgg gctgtcgggg cggtcgagca gccagaccca atgcggattg acgtcgagca
     2701 gcacccaggt gcgctggccg agcgcgccgc tcaaatgcgc cacggatgtg caaacggtaa
     2761 tgacgagatc gagcgcgctg ataaacgcgg cggtgtcgtc gaaggtggcg aattcggcgg
     2821 tgaagtccga catcggcaga ccggccgcct gggccgccgc gacgtccgcc tcggcacctg
     2881 gttgcaacga atagaacgcc acgtcctgca tgccgccgaa atgtgccgcg tagcgttccc
     2941 aacccacgcg ccggaatgga ttgcgctgat ggccggggct gccggtccat gtgagtccga
     3001 ctttgagcct cgcttcgcca gcaagcctct gctgccatga agcactggct gccgcatccg
     3061 gacgcaaata cggcgtcgcg gcggggatgg tctcttcgcg cgtgtcgaat agcaagggca
     3121 ggctgagtaa tggaatttcg tagtcgaacg gcggcagaga ttcggcgccg ccgccggccg
     3181 aatagtcatt cacatgagcg ccgaggctgc gggcgagcag cgcccccatt tgcggaaatg
     3241 agttccacac aagacggccg ccttcgcgac gaacacgttc ggccaacaga gggatatagc
     3301 ggctgaactg cagcacatcg cccatgccct gttcacccca caccaacagc gttttgccgg
     3361 cgagcggctc gccttgccag gtcggcgccg gcatcgctgg acggttgccg cccaactcaa
     3421 gcgaaccatc ccaacgtgcc tcgtgagctt gccagccgtg cgcgtaattg ccgcgcatca
     3481 ggagcagcat gccgagattc gagcgcatgg tcgcgtcgtt gggcgctaat gcacaggcgg
     3541 ttctttcggc gtgctccgct tcggcccagc gttgcgcttc acgcagcgcc agcgcatagt
     3601 tgttatgggc cagcgggcta tgcggcgtgc attgcacggc tcttgcgccg gcttcgagcg
     3661 cgcgcgagag attcaaattg gcgcggcttg cctgagtgag attagtccac gcgtcgccat
     3721 ggtcggggtc gtcatgaacc gcactttcca gtagagcgag ctgctcgttc gggtcgccac
     3781 cagtgagggt aagggcgccg gcgagattgt tgcgcaactg cggcagtgtc ggatcgatgg
     3841 cgagcgcgcg acgataggcc gcgattgctt cgccatgccg gtttgccatc tgcagcgcgt
     3901 agccgaaacg gaagtgcgca agagcgcgat gcggatcgag ttgggcaata atggcggcga
     3961 gttcgatcgc gtcgttgtga cgatgttgct cgagcagcgc aacaatgagc gattcgattg
     4021 acgcgtcttc gagtgcgtgg aattgcgcgg cggcatcgag ccaactggcc tccgcttccg
     4081 cgctgccgtt ttgccgggct gcggcggcat gtcgcagaaa agtcaggaaa gctgacgagg
     4141 cgggtgtcgc gctatcagag gtggggagca tggatgggtt gggcggcgca agaaccggtg
     4201 ataagcgatc ggcctacgca acgctgtcgc aaaaatgcca tgttaccggc gcgaagccgc
     4261 gaggtaagtc ggactagtcc gaacaggtaa actaaagtca tgcatcgtat aaccaacacc
     4321 ggccgggtca acctcagcaa tctgttctgg ctacgtagcc tcgccatcat cggccagctg
     4381 atgacgatcg cattcgtgca gatcttcatc ggcgtgcatc tgccgttgcc tgcgatgttg
     4441 ctcgtgatcg ccctcgaggt gatcttcaat ggacttacgt ggtggcgtgt ttcgcagcag
     4501 cggctcgagt ccaatctcga actgttcggc cagatctggg tcgacctcgg cgcgttgtcg
     4561 gcgctgctgt tcctctccgg tgggaccaac aacccattcg tttcgctgta cctgccctcg
     4621 ctcgcgattg ccgcagccgt gttgccatgg catctgatgg cgtggctcgc cgcattcgcc
     4681 gtggcctgct atgccgtgct tggtttcgat tccgtgccgc tgaatctcga caacccggcg
     4741 aacctgttcg actactatcg tgccggcatg tgggtgaact tcatggtcag cgtcgggctg
     4801 attgcgtggt tcgtcgcacg catgtcacgc gcactgcggc tacgtgacgc agcgctcgga
     4861 gacgcgcagc agcgcttgct tcacgacgaa cgcgctgtcg cgctcggcgt gcaggccgcc
     4921 actgtcgcgc acgaaatcgg cacgccgctg tctacaattg ccatgttatc cgaagaattg
     4981 cgcgacgccg cgcgaaccga taagggactg gcgccgtaca gcgccgatct ggagttgctc
     5041 gaacagcaaa tgacgctgtg tacgtcggcg cttgcgcgac tgcgcagccg tgcatcgaca
     5101 acgaccaccc agcaaacggt cggcgaatgg ctcgagtcgt tcggcgagca atggcgctta
     5161 cgtcatccgc atgtgaagtt cgagcggatc ggtgtgccgc ccgcgggtgt cagccttgac
     5221 gatacggttg ccgtcagtca gattctcacg attctgctcg acaacgccgc gcgcgcgagt
     5281 cgcgatcacg tgacgctgtc gtgcaggctc tcggcgcgtg gcgaccaggt tgtattcgag
     5341 gtctgtgacg cgggccctgg tattccggct gcgttgcgcg ggtcgctcgg cacgatgccg
     5401 gtggacagca cacagggtgg gcacggcgtg ggcctatatc tggcattttc agcggcggca
     5461 cgtctgaagg gatcgatcga gctgaacgat gtcagcgaga ttaaaccgcg tggcacgcgt
     5521 gcagtgttga gactgccgct cgccgggcga aaattatcag gtgcgggccg tcaaggcact
     5581 tcgccatcca acacggagaa acaggcatga gcgatatgaa ttttctggtg atcgacgacg
     5641 acgaggtgtt ctccggtatc ctcgcccgcg gtttgacgcg gcgcgggtat acggtggccg
     5701 aagcgcacaa cgcggctgag gcgatcaagt tggcgaacca gcagaagttc ggccagatca
     5761 cggtagacct gcatctcggc aacgactccg gtcttacgct ggttgcccct ttgcgtgatt
     5821 tgcagcctga cgcgcgcatg ctggtgttga ccggttatgc cagcatcgcc acggcggtgc
     5881 aggcggtgaa agacggtgcc gataactacc tggcaaagcc tgccaatgtc gagacgattt
     5941 tgtcggcgct gcaaagcgag gcgagtgcgt tgcaagctga cgaagcgatt gagcatccca
     6001 cgccgttgtc ggttgcgcgc ctggaatggg agcatattca gcgcgtgctc gcggagcacg
     6061 gtggcaatat ttcggccact gcgcgcgcgt tgaacatgca tcgtcgaacg ctgcagcgga
     6121 agttggcgaa gcggccggtc aggcagtaag caatcggtct cgcttgggcg ctgcttgcac
     6181 ttgcgggcag gaaagcaaaa cgggctgtct ttcacaagac agcccgtttt gttacgcctt
     6241 aggcatggtc ggccacgtgc gaggtatttg gctttaacgc ggcattggta tcaacgttga
     6301 cattagcgtc aacgcccgca ttgatacgaa cgccgatgtc gatatcgata tcgacatcgg
     6361 catcacagca cgtagcgcga caggtcctcg tcttccgcca cttcgttcag cgcgcgatcg
     6421 acatacgccg cgtcgatctg caccgtcttg cccgagtgat tgccggccgc gaacgagaca
     6481 tcttcgagca gtttttcgat cactgtgtac aggcggcgtg cgccgatgtt ttcggtcttc
     6541 tcgttgaccg agtacgcgat ttcggccata cgtcgaatgc cgtcgtcggc gaattcgagg
     6601 tgcacgtctt ccgtggccag cagtgcctgg tattgcttga cgaggctcgc gtcggtcgac
     6661 accaggatcg attcgaaatc gcccaccgac agcgaatcca gttcgacccg aatcgggaag
     6721 cggccttgca actccggaat cagatcgctc ggcttggcca gatgaaacgc gccgctcgcg
     6781 atgaacagaa tgtggtcagt cttcaccatg ccgtacttgg tgttgatcgt ggtgccttcg
     6841 acgagcggca gcagatcgcg ctgcacgccc tggcgcgaca cttcgccacc gccggcttca
     6901 ttgcgcgacg cgatcttgtc gatttcgtcg agaaacacga taccgttctg ttcgacgttc
     6961 tgcaccgcct tggctttcac ctcttcgtcg ttgagcatct tgccggcttc ttcgtcggtg
     7021 aggaccttaa gcgcttcctt caccttcagc ttgcggcgcg ttttcttgcc gccgccgatg
     7081 tttgcgaaca tcgagcgaat ctgctcggtc atgtcttcca tgcccggcgg ccccatgatg
     7141 tccatgccga cttgcggctg ttcgacgtcg agttcgattt ccttatcgtc gagcaggcct
     7201 tcgcgcagac gcttgcggaa agtctgacgc gtggtgctgc cttcgtcgac ggtatccgcc
     7261 gcgctcgagc tcgcgccgaa accaaccggc cgcgcgctcg gcaacaggat gtccagaacg
     7321 cggtcttcgg cctgatcttc agccttggtt cgcactttgc gcatttccgt ttcgcgcgtc
     7381 tgcttgaccg agatttcgat cagatcgcgc acgatgctgt cgacgtcgcg gccgacataa
     7441 ccgacttcag tgaacttggt ggcttcgatc ttgatgaacg gcgcgtcggc cagtttcgcc
     7501 agacgccgcg cgatttcggt tttgccgacg ccggtcggtc cgatcatcag aatgttcttc
     7561 ggcgtgattt cctgacgcag cggatcttca acctgctgac ggcgccagcg gttacgcagg
     7621 gccacggcaa ccgctttctt cgcgcggcct tggccgatga tgtgtttgtc gagttccgag
     7681 acgatctcgg caggggtcat ggtgctcatc gtgggtcctt actcgatcgt ctcgatgacg
     7741 cggttgtggt tcgtatagat gcacatgtcg ccggcaatct ccagcgactt ctccacgatt
     7801 tcgcgcggcg acatctcggt gttgtcagtg agcgcctttg cagcagcctg tgcgtaagcg
     7861 ccgcccgaac cgatcgcgca gatgccgcct tccggatcga gcacgtcgcc gttaccggtg
     7921 atgacgaggg tggtagtggc atccgcggcg atcagcattg cttcgaggcg gcgcagcatg
     7981 cggtcggtgc gccagtcttt ggccagctcg acggcggcgc gagtcagatt gccctgatgt
     8041 ttttccagct tcgcttcgaa gcggtcgagc agcgaaaagg cgtcggccgt gccgccggca
     8101 aagccgacca gcaccttgcc gttatagatg cgccggactt tcttcgcacc gcctttcatg
     8161 acgatgttgc ccagcgtcac ctggccgtcg ccgccaagcg cgaccttgtc gccgcggcgc
     8221 acggaaacga tcgtcgtgcc gtgaaattgc tccatatgcg ttcctctttg caaaacgggt
     8281 aggacgcggt ggcgcagcac caccgcctga atcctggaag cggacggcgc gcaaagctga
     8341 aacgatcaac tcgccggcgc gcgctcgtgc aacgcatgtc cggcttattt tagggcgtgc
     8401 gcggtcatat caagagccgg caaatggcat aagacaaaaa cacgggattc ggcgcgggga
     8461 actgtgcgca agggcgcaca aaagaaaaaa ggcgcacaga tcaccgtgcg cctctcgacg
     8521 aagcagctta aaagggcgcg gagccgaagc cgcgccgcgg gcgttcagtc gccgaacagc
     8581 ttctggcgca gttcgcggcg ttcctgtgct tcgagcgaca gggtggccgt gggccgtgcg
     8641 agcagacgcg ggatgccaat cggctcgccg gtttcttcgc accagccgta gtcgcccgaa
     8701 tcgatgcgcg cgatcgattg ctgcaccttc ttcagcagct tgcgctcgcg gtcgcgcgtg
     8761 cgcagttcga gcgcatgctc ttcctcgatc gttgcgcggt cggccgggtc cggcacgatg
     8821 actgtttcgc gcaggttctc ggtcgtctgg ccggcattgc ggagaatgtc cgcttgcagc
     8881 tgttcgagct tgttcttgaa gaaggcgagc tgatcctcat tcatgtaatc cttgtcgctc
     8941 atcttcagga tttcggcttc ggtcaagagt cgtttcgtcg tcatctggct tgcttcttca
     9001 atgtgaggca aaactcttac atagtgcccg cactttcgta ttgcccgcaa aggcgcctcg
     9061 aggacgcgga gcgccagggc gggcacggac ggtggactgt cgtgacgccg aagcgcctgg
     9121 cgcccacacg ccgcggcgtc gctacgccaa tgcggggccg aactcctctg gaaaccgatt
     9181 ctcaaatgct cctgaggcat tgcttgccgg cagcgcgcct tatcagctgt gccagccggc
     9241 attgtcggcg tgatgtccgg acaagatttt ccggcgccgt ttca
//
LOCUS       DATASH010000470         6099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6403098,
            whole genome shotgun sequence.
ACCESSION   DATASH010000470 DATASH010000000
VERSION     DATASH010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6099
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6403098"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1102)
                     /locus_tag="VHV99_13000"
                     /pseudo
     CDS             complement(1..1102)
                     /locus_tag="VHV99_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182103.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
     gene            complement(1308..3182)
                     /locus_tag="VHV99_13005"
     CDS             complement(1308..3182)
                     /locus_tag="VHV99_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX3636388.1"
                     /translation="MPLPTPPSKLGNLLPPDEYKAKAAAPARSAAKKQATEGESAESA
                     DYGRANVATPMAHAANAATTLRPVPASSASPAASATAEQAAPARTRRSKQTAALLQPV
                     PASRPQAEPAAQAAPAAKSQPVVARAPSAKGPAASTPAAAAPSARGTSKKRGASTDPL
                     EVQKLFVLDTNVLMHDPSCLFRFEEHDVYLPMMTLEELDNHKKGMSEVARNARQVSRT
                     LDALVANAGNMSDGISLARLGSREASGRLYFQTKLTAIEPVEGLPEGKADNQILGVVR
                     ALQRDRMDRQVVLVSKDINMRIKAHALGLPAEDYFNDQVLEDSDLLYSGIRALPQDFW
                     TKHAKGMESWQDTKTGTTYYRVTGPLCASMLVNEFVYLEPQNGEPAFHALVRELNGKT
                     ALLQTLRDYGHHKNNVWGITARNREQNFALNLLMNPEIDFVTLLGQAGTGKTLVALAA
                     GLAQVLDDKRYNEIIVTRATVPVGEDIGFLPGTEEEKMQPWMGAFDDNLEVLQKTDDA
                     AGEWGRAATQELIRSRLKVKSMNFMRGRTFVDKYLIIDEAQNLTPKQMKTLVTRAGPG
                     TKIICLGNIAQIDTPYLTEGSSGLTYVVDRFKGWAHSGHVTLARGERSRLADYASEIL
                     "
     gene            complement(3529..3990)
                     /locus_tag="VHV99_13010"
     CDS             complement(3529..3990)
                     /locus_tag="VHV99_13010"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011549652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX3636389.1"
                     /translation="MPIAVDQPIPDFTAPATGGDITLSRLRGKKVVLYFYPKDNTPGC
                     TTEGLQFRDLYPKFRKAGAEILGVSRDSLRSHDNFKAKLELPFPLISDPEETLCALFG
                     VMKLKKMYGKEVRGIERSTFLIDAEGVLRQEWRGVKVPGHVDDILEAVQAL"
     gene            complement(4144..5037)
                     /locus_tag="VHV99_13015"
     CDS             complement(4144..5037)
                     /locus_tag="VHV99_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX3636390.1"
                     /translation="MARIVLKIDVDTLRGTREGVPNLARIFDRFKARATFLFSLGPDH
                     TGWAMRRVLRPGFLKKVSRTSVVEHYGIKQLMYGVLLPGPDIGVKTAAEMRAIHEAGF
                     ECGIHTWDHVYWQDNVRSQDREWTEKQMQRSFNRFVAIFGAPPVTHGAAGWQMNGHAF
                     EQIDAWGMHYASDGRGHSPYLPVVGGRQLTHVQMPTTLPTLDEVLGVDGVDEHNVAAW
                     MLKHTENNPHDQVFTLHAELEGQKLAPIFEQLLAGWRAQGHTFATMGDYYATLDRSTL
                     PSYPVTWGEIPGRSGELIVQP"
     gene            complement(5048..6094)
                     /locus_tag="VHV99_13020"
     CDS             complement(5048..6094)
                     /locus_tag="VHV99_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-keto-pentose/UDP-xylose
                     synthase"
                     /protein_id="HEX3636391.1"
                     /translation="MKKVLILGVNGFIGHHLSKRILETTDWEVFGMDMQTERLGDLVN
                     HERMHFFEGDITINKEWVEYHIKKCDVILPLVAIATPATYVKQPLRVFELDFEANLPI
                     VRSAVKYGKHLVFPSTSEVYGMCTDEQFDPEESQLSYGPINKPRWIYACSKQLMDRVI
                     WGYGMEGLNFTLFRPFNWIGPGLDSIYTPKEGSSRVVTQFLGHIVRGENISLVDGGAQ
                     KRAFTDIDDGIGALMKIIENKDGVATGKIYNIGNPTNNFSVRELAHKMLTLAAEFPEY
                     ADLAKKVQLVETSSGAYYGAGYQDVQNRVPKIDNTMQELGWAPQSTFDEALRKIFEAY
                     RGHVGEARALVEQQ"
BASE COUNT         1056 a         1877 c         1937 g         1229 t
ORIGIN      
        1 cgactgaagc aggcggaggc ggctcgaatg cgtcggctgc ggcgtcgatc ggatccggcg
       61 cgcgggcggc agccgttgac ggttcggcgc gtgcggccgc cgtctgctga ttcgtcaatc
      121 tcgcagcggt gtcatacgta ctcgcccggc cggtctgaac gggcagcgga gacaccgcac
      181 ccgccgcgcg cgcttgcatt tgcgcggtgc tcatcccggg cggcggcggt tcgaacggat
      241 ccgcttgcgg tgccggtgcc gcagctgtgg cgaccgtcgg ctgagcagtt gcagtcagcg
      301 gtgcacgcgg ggttgccgcg acacgagccg tttgctgcgg agccatcggg ttcgcggcat
      361 agcctgaagc ttgcgacgca acaggcgctg ccgcatagcc cgccgagcgc gtcgccgcgg
      421 cgttcgccac gtagcccggc gaagctcgca tcgcctctgc ggcgcccgca taagccggcg
      481 ctgtctgagc caccggcgcg acgcgctcgg gttgtggcgc ggcagcctga tagttcggcg
      541 tatcgaatgg cgctggcgtc gcggccggcg ccgccacacg acgaataccg tcaaagcgct
      601 tggcccaata cggattggtc agatagtcga gccgcaccgt gccgcctgtc gacggtgcgt
      661 tgacgaagcg cagcttgccc acgtaaatgc ccacatgcga atgcgcgcgc ccggtggtgt
      721 tgaagaaaat cagatcgccc ggggcgattt cgtccggctc aatcgattcg ccgcgcccgc
      781 tcatatccgc ggtagtccgc ggcagattga cggaagcggc gcgcgccacc acatagcgaa
      841 ccaatccgct gcaatcgaag ccgctatcgg gcgtattacc gccccaacgg tacggaatgc
      901 cgaccagact catcgcctga atcgaaattt cttcacggcc gacactgtga tcgacaaagt
      961 tcgggaagcc cggcggcggc gcgtgataag cgccattcgc gacgacgacc gattaccccg
     1021 gttcgcgcga cgtcttttgt ggtgcgccgg cgcaagcggc gagcagcaaa acggtcagca
     1081 gcgaaagggc aagtcggcac atgaagacga cgcgagcggt aaacgctcgt ttaatggcgg
     1141 atgatttcgg gatggtagcc cgtggaacct ggctcaggca agaattcttg acaacttact
     1201 tgaagtttgt gcgctaagtg ttgcccatgc ggaacgattc aaccagaaaa aagccgcgcc
     1261 cggaaccaac cggacgcggc ttccgctcga agctaagaca ttgaaactta aagaatttcc
     1321 gacgcgtagt ccgccagacg cgagcgctcg ccgcgggcca gcgtcacgtg cccgctgtgc
     1381 gcccagccct tgaagcggtc gaccacgtag gtcagacccg agctgccttc cgtcaggtaa
     1441 ggcgtgtcga tctgcgcgat attgcccaga cagatgatct tcgtgcccgg acccgcgcgc
     1501 gtgaccagtg tcttcatctg cttcggcgtc aggttttgcg cttcgtcgat gatcagatat
     1561 ttgtccacga acgtacggcc gcgcatgaaa ttcatgctct tgaccttcag gcgcgagcgg
     1621 atcagctcct gagtggcggc gcggccccat tcgccggcgg cgtcgtcggt tttctgcagg
     1681 acttcgaggt tgtcgtcgaa tgcacccatc cacggctgca ttttttcctc ttccgtaccc
     1741 ggcagaaagc cgatgtcttc accgaccggc actgttgcgc gcgtcacgat gatctcgttg
     1801 tagcgcttgt cgtcgagcac ctgagcgaga cctgccgcca gcgcgaccag cgtcttgccg
     1861 gtaccggcct gacccagcag cgtgacgaag tcgatctccg gattcatcag caggttcagc
     1921 gcgaagttct gctcgcgatt gcgtgccgtg atgccccaca cgttgttctt gtggtgcccg
     1981 tagtcgcgca gggtttgcag cagcgcggtc ttgccgttca gctcgcgcac cagcgcatga
     2041 aacgccggct cgccgttctg cggctccaga tagacgaact cgttgaccag catcgaggcg
     2101 catagcggac ccgtcacccg gtaatacgtg gtaccggttt tggtgtcctg ccagctctcc
     2161 atgcccttcg cgtgcttggt ccagaaatcc tgcggcagcg cgcggatgcc cgaatacagc
     2221 agatcgctgt cttcgagcac ctggtcgttg aagtaatctt ccgcgggcag gccgagcgca
     2281 tgcgccttga tgcgcatgtt gatgtctttc gacaccagta cgacctggcg atccatccgg
     2341 tcgcgctgca gcgcgcgcac cacgccgaga atctggttgt cggccttgcc ttccggcaga
     2401 ccttcgaccg gctcgatggc ggtgagcttg gtctggaagt acaaacgccc ggacgcctcg
     2461 cggctgccga gacgcgcaag cgaaataccg tcggacatat tgccggcgtt cgccaccaac
     2521 gcgtccagcg tgcggctcac ctgacgagcg ttacgcgcga cttccgacat gcccttttta
     2581 tgattgtcga gttcttccaa cgtcatcatc ggcagataga cgtcgtgttc ctcgaaacgg
     2641 aacaggcagc ttggatcgtg catcagcacg ttcgtgtcca gcacgaagag cttctggact
     2701 tcaagcggat cggtggatgc gccgcgcttc ttgctggtgc cgcgcgcgct gggtgcggcg
     2761 gctgcgggcg tgctcgcggc cgggcctttc gcgcttggcg cgcgcgcgac cactggctgc
     2821 gatttggcag caggtgcggc ttgagccgct ggttcggctt gcggacggga agcgggaacc
     2881 ggttgcagca atgcggcggt ctgcttcgac ctgcgtgtgc gcgccggtgc agcttgctcg
     2941 gcggttgcag acgcagcagg ggaagccgac gatgccggaa cgggccgcaa ggtggtcgcg
     3001 gcattggcgg cgtgtgccat tggcgtcgcg acattcgcgc gaccgtagtc ggccgactcg
     3061 gcggattcgc cttcggttgc ctgtttcttc gcggcggagc gcgccggcgc ggcggcttta
     3121 gccttgtatt cgtcaggcgg caggagattg ccgagcttgc tggggggggt aggcaaaggc
     3181 atggtttccc tcgaataatc gggtcacatt ttcgtgcgcc gcctgtcgca ttctgccggt
     3241 gccagtgatt gcgcacgcag tttgcgcccg gaggcgcgca ttcggtctag agatgccggt
     3301 tcgcctggtc ttcgatcggc tccttaacgg aagcgcgtgg ccgagtgaac gaacggctgc
     3361 gcgcaattaa aaaagccgcc gccccgcgta cgggacaagc ggctcgcctt cggttgtcgc
     3421 gttgcacctc atgacttcga cggaaccgat aagacggccc tcaagtctgg cgcagctgcg
     3481 aaaagtgtgg ggtggacagg cactcattgg aacccaatat aacgcgcctc aaagcgcttg
     3541 tacagcttcc agaatgtcat cgacatgtcc tggcactttc acgccgcgcc actcctggcg
     3601 cagcacgccc tcggcatcga tcaggaacgt ggagcgttcg atcccacgta cttctttgcc
     3661 atacattttc ttcaatttca tgacgccgaa gagcgcacac aaggtttcct cggggtccga
     3721 gatcaacgga aacggcaatt cgagctttgc cttgaagtta tcatgggagc gcaggctgtc
     3781 gcgcgaaacg ccgagaattt ccgcgcctgc cttcctgaac ttcggatata agtcgcggaa
     3841 ttgcaggccc tcggtcgtac agcccggcgt gttgtccttc ggatagaaat acagcaccac
     3901 cttcttgccc cgcagcctgg acagcgtgat gtcgccaccg gtagcggggg cggtaaagtc
     3961 ggggatgggt tggtcgactg cgatgggcac gaggttctcc ggttgttatg gctggcgccg
     4021 cgcttgcaac ggctgcggcg tcggcctggc atcgaggctt gggggaggtc gactgccgcg
     4081 cggtgtacgc tcgcgcggcg gtcgggtcat caggtttgtc ggcaccggct atcgcctggc
     4141 cagtcagggc tggacaatca actcgccgga gcggcctgga atttcgcccc acgtaacagg
     4201 gtacgatggc agcgtgctgc ggtctagcgt ggcgtagtaa tcacccatgg tcgcgaaggt
     4261 atggccttgc gcgcgccagc ccgcgaggag ctgctcgaaa atcggggcga gcttttgtcc
     4321 ttcgagctcc gcgtgcagcg tgaacacctg atcgtgcgga ttgttttcag tgtgcttcaa
     4381 catccatgcg gcgacgttgt gctcatctac gccatccacg ccgagcactt cgtcgagcgt
     4441 gggcagcgtg gtgggcatct gcacatgcgt tagttgcctg ccgccgacca ccggcagata
     4501 gggcgagtgg ccacgcccgt cggacgcgta gtgcatgccc cacgcgtcga tctgctcaaa
     4561 cgcatggccg ttcatctgcc agcccgccgc gccgtgcgta acgggcggcg cgccgaagat
     4621 cgcgacaaaa cggttgaagc tcctctgcat ctgcttctcg gtccactcgc ggtcttgcga
     4681 acgtacgttg tcctgccaat acacgtgatc ccacgtgtgg atgccacatt cgaaaccggc
     4741 ttcatgaatc gcgcgcattt ccgcggcggt cttcacgccg atgtccggcc ccggcagcag
     4801 cacgccgtac atcaattgct tgatgccgta atgttcgacc accgaggtgc gcgacacctt
     4861 cttcagaaag cccggccgca atacgcggcg catcgcccaa cccgtgtggt cgggcccgag
     4921 gctaaagagg aacgtggcgc gtgccttgaa gcggtcgaaa atgcgcgcga gattcggcac
     4981 gccttcgcgg gtgccgcgca gcgtgtcgac gtcgatcttc aggacgatac gagccaagga
     5041 tcaggcctta ttgttgttcg acgagcgcgc gggcttcgcc cacatggccg cgatacgctt
     5101 cgaaaatctt gcgcaacgct tcgtcgaagg tcgactgcgg cgcccagccg agctcctgca
     5161 tggtgttgtc gatcttcggt acgcggttct gcacgtcctg gtagcccgcg ccgtagtacg
     5221 cgcctgagga cgtttcgacc agttgcacct tctttgcgag gtcggcgtat tccgggaatt
     5281 cggcggcgag cgtcagcatc ttgtgcgcga gctcgcgcac cgagaagttg ttggtcgggt
     5341 tgccgatgtt gtagatcttg cccgtggcga cgccatcctt gttttcgatg atcttcatca
     5401 gcgcgccgat gccgtcgtcg atatccgtga aagcgcgctt ttgtgcgccg ccgtccacga
     5461 ggctgatgtt ctcgccgcgc acgatatggc cgaggaactg cgtgaccacg cgcgagctgc
     5521 cttccttcgg tgtgtagatc gaatcgaggc ccgggccgat ccagttgaac ggacggaaca
     5581 gcgtgaagtt caggccttcc atgccgtaac cccagatcac gcggtccatc agctgcttgg
     5641 agcacgcgta gatccagcgc ggcttgttga tcgggccgta cgacagttgc gactcttccg
     5701 gatcgaactg ctcgtccgtg cacatgccgt agacctcgga ggtggacggg aacacgaggt
     5761 gcttgccgta cttgacggcc gaacgcacga tcggcaggtt cgcctcgaag tcgagttcga
     5821 acacgcgcag cggttgctta acgtacgtgg cgggcgtcgc gatcgcgacc agcggcagga
     5881 tcacatcgca cttcttgatg tgatactcga cccactcctt gttgatcgtg atgtcgcctt
     5941 cgaagaagtg catccgctca tgattgacga ggtcgcccag acgttcggtc tgcatgtcca
     6001 tcccgaagac ttcccaatcg gtcgtttcga gaatgcgttt ggacaggtga tggccgatga
     6061 agccgttcac acccagaatc aggacttttt tcatgaatg
//
LOCUS       DATASH010000471         6204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3832597,
            whole genome shotgun sequence.
ACCESSION   DATASH010000471 DATASH010000000
VERSION     DATASH010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6204
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3832597"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(406..1119)
                     /locus_tag="VHV99_13025"
     CDS             complement(406..1119)
                     /locus_tag="VHV99_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3636392.1"
                     /translation="MTALLDIQQLNAWYGASQALRGVSLQIGAGEVVALVGRNGSGRS
                     TLARAIMGLVRSEGKLCFAGHALSGLRTFEIARLGLGYVPEHRDVFPTLSVHENLQLG
                     VAPRARGRAPHFAFDDAYQLFPVLRERKRTRAGALSGGEQQMLTLARALLGDPDLLVI
                     DEPGEGLASQVIAQVAQCLRMLRDRGVAMLLIEQRLVIAQDIANRVAVMGHGEIVFDD
                     TLALFVKRPDVMREWLGVG"
     gene            complement(1116..1868)
                     /locus_tag="VHV99_13030"
     CDS             complement(1116..1868)
                     /locus_tag="VHV99_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3636393.1"
                     /translation="MIPALALYGIEKRFGATQILRGVDLAIEPGERHALIGPNGAGKS
                     TLFNLIAGGARPNAGRIDLFGAEITRLAPAAITRRGLARSFQTTSVFARLSVFDNLRC
                     AAMLAERDRTRWWQRFSGSRTVDARADQVLDVVGLSTRRHSQAGTLSYAEQRALDLGI
                     ALAGGAHTLLLDEPTAGMNRAEAARAIELIRSTTAGRTLLMVEHDMDAVFALADRISV
                     LVQGRIIATGTPAAIRADAAVRAAYLGDDFRP"
     gene            complement(1865..3127)
                     /locus_tag="VHV99_13035"
     CDS             complement(1865..3127)
                     /locus_tag="VHV99_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX3636394.1"
                     /translation="MAAATSRSMPRIVAPWLALVLFLAVPPLVWPQSWLLAYLAQTAT
                     MIVFALSYNLLLGETGLLSFGHAAYSGLGALIAAQVFNRIGVPLVLLPLIGGLGGALC
                     GVLFGFVSTRRAGTAFAMITLGIGELVAAAAWTLPDWFGGEAGVPIDRASGPLLASWS
                     FGPAREAYALIALWCVLASVAMFALSRTPFMRLANAVRDNPARAAAIGCYPRRVRYGV
                     VVMSAFFAGIAGTLGLINVELVSTESVGMMRSGAVLIATVIGGTAAFFGPVAGAIVLT
                     FFSVAVASVTRAWPFYLGLFFIVIVMASPDGLAGFAQRHTARLSGYGWRVCSKAYLWG
                     VASAVLWTLAVVLAVQWAYAVQFGADDGAAFNVIGVPLATASPQFWLGGAVFMLAIAG
                     ALAARYALRAQARLAVRASAAPSTGDAR"
     gene            complement(3147..4085)
                     /locus_tag="VHV99_13040"
     CDS             complement(3147..4085)
                     /locus_tag="VHV99_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX3636395.1"
                     /translation="MQSFVINLLNGVSYGLLLFMLSAGLTLIFSMLGVLNFAHASFYM
                     LGAYVGFSVAAHAGFWSALVIAPLVVGLIGAALERWLLRRVRVHGHLPELLLTFGAAY
                     LLGELVKLGWGLSPLNATVPAALDGPLFTVYGAAFARYRAFMMAVSLAMLAVLYAVLR
                     VSKAGLIVRAALTHASAVEALGHNVPRVFTGVFAAGTALAALAGVIGAPLFVIEPAMA
                     ESLGSIVFVVVVIGGLGSLSGALAASLIVGCVQTFAVASDVSLGDLTWAFGGALPAEW
                     DALTLAQFAPLIPYLLLVAMLALRPRGLFGRRDDHA"
     gene            complement(4090..4539)
                     /locus_tag="VHV99_13045"
     CDS             complement(4090..4539)
                     /locus_tag="VHV99_13045"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX3636396.1"
                     /translation="MNKPTPATRGAYPHFLPITTRWMDNDVYGHVNNVVYYSYFDTVV
                     NEYLIRAGVLDFEHGATIGLVVETHCNYFAPLVFPERVEAGLRVVRLGSSSVRYEVGL
                     FKDGDDQPAAQGHFVHVYVDRVTRRPVTLPAELRAALEPLTVAMQAD"
     gene            complement(4581..5111)
                     /locus_tag="VHV99_13050"
     CDS             complement(4581..5111)
                     /locus_tag="VHV99_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX3636397.1"
                     /translation="MTEYQFCPRCATPLIERADAEHEGGRIRQICPDNECGYVHWNNP
                     LPVVAAIVEYEGKILLARNAAWPEGMFALITGFLENGETPEEGIAREVLEETSLRAET
                     VELIGVYEFIRKNELIIAYHVKAYGTVALSPELLEYRLVEPAKLRPWRAGTGQALGEW
                     MRRRGLPFEFVERPGQ"
     gene            complement(5239..>6204)
                     /locus_tag="VHV99_13055"
     CDS             complement(5239..>6204)
                     /locus_tag="VHV99_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HEX3636398.1"
                     /translation="ANNSGHEPAYGDDSWTNQVCDRLRDLFQTDCEVFFVFNGTAANS
                     LALASLCQSYHSVICHELAHIETDECGGPEFFSGGSKLLTAPGIGGKLTPNAIEAVVT
                     RRADIHYPKPKVVTLTQSTEVGTVYTVEEVRAIAAIAKRRQLKVHMDGARFANAVAAL
                     DVHPSEITWRAGVDVLCFGGTKNGLPVGEAVVFFDRSLADDFAYRLKQAGQLASKMRF
                     ISAPWLGLLDNDVWLRNARHANAMADLLQTRLQEIPGVSIMFPRESNAVFAQLPPQAA
                     KAMRARGWKFYEFIGAGGCRLMCAWDTQSETVERFAAEVRELCAA"
BASE COUNT         1257 a         2110 c         1910 g          927 t
ORIGIN      
        1 gtcaccaggg ctgaatgtgc gagggcacag attccagatg caccactacc gcgactgcgc
       61 gggggacccg cttatgcgct ggcggggtga gccgctttct ttgcttatct ttctttgcgg
      121 cggcaaagaa agtaagtgcc gccccgcaca ggggcaacgc taatagacca ccaacaaatc
      181 aaggaaaggc caacaccaca aaagcccaga caaaaaagcg ccacgtaggc aaccacatca
      241 aaaaaagccc gccaccttaa ccacgcgtga cgacgcgcat caggacaacc aaacagccac
      301 aaattcgaat gcaacgctgc aatacaacca cagggagaca cccgcaaaaa acgctgctgt
      361 tcggctttaa cagacccacc ttgcgcaccg tggaaccgca actacctacc ccacgccgag
      421 ccattcccgc atcacgtccg gtcgcttcac aaacaacgca agcgtgtcat cgaacacaat
      481 ttcgccatgt cccatcaccg ccacgcgatt agcgatgtct tgcgcaatca caagccgttg
      541 ttcgatcagc agcatcgcca caccccggtc acgcagcatc cgcagacatt gcgcgacctg
      601 cgcgatcacc tggctggcca gtccctcgcc gggttcgtcg atgaccagca ggtcgggatc
      661 gccgagcaat gcccgggcaa gcgtcagcat ctgctgttca ccgccggata gcgcgccggc
      721 gcgcgtgcgc tttctttcgc gcaggacagg aaacagttgg taggcgtcat caaaggcaaa
      781 gtgcggggcg cggccgcgag cccgcggcgc cacaccgagt tgcagatttt cgtggacgct
      841 cagcgtcgga aagacgtcgc gatgttccgg cacgtaaccg agtcccaggc gggcgatctc
      901 gaaggtgcgc agcccgctga gtgcatggcc ggcaaaacac aacttgccct cgctgcgcac
      961 gaggcccatg atcgcgcgcg ccagcgtcga gcgtccggag ccgttgcgcc cgaccagcgc
     1021 gacgacctct cccgcgccga tctgcaacga cacgccacgc agggcctggc tcgcgccgta
     1081 ccacgcattc aattgctgaa tgtcgagcag ggcggtcatg ggcggaagtc gtcgccgaga
     1141 taagcggcgc gcactgccgc gtccgcgcga atcgccgccg gcgtaccagt cgcgatgatg
     1201 cggccttgca ccagcaccga aatgcgatcg gcgagcgcga acacggcgtc catgtcgtgc
     1261 tcgaccatca gcagcgtgcg gcccgcggtg gtcgagcgaa tcagctcgat ggcgcgtgcc
     1321 gcttcggcgc ggttcatgcc ggcggtaggt tcgtcgagca gcaaggtatg cgcgccaccc
     1381 gcgagggcaa tgccaaggtc gagcgcgcgc tgctcggcat agctcagtgt gccggcctga
     1441 ctatgccggc gcgtactcag tcccactaca tcgagcacct gatcggcgcg tgcatcgacc
     1501 gtgcgtgaac cgctgaaacg ctgccaccag cgcgtgcggt cgcgctcggc cagcatggcc
     1561 gcacagcgca gattgtcgaa cacgctgagg cgagcgaaca cactggtggt ctggaaactg
     1621 cgcgcgaggc cacggcgcgt gatcgcggcc ggcgcgagac gcgtgatctc cgcaccgaac
     1681 agatcgatgc gcccggcgtt cggacgcgcg ccgccggcga tcagattgaa gagcgtcgat
     1741 ttgccggcgc cgttcgggcc gatcaacgca tggcgctcac ctggctcgat agcaagatcg
     1801 acgccgcgca gaatctgcgt ggcgccaaag cgcttttcga tgccgtagag cgcgagggcc
     1861 gggatcatcg tgcgtctccg gtgctcggcg cagcactggc gcgaacggca agacgtgctt
     1921 gcgcgcgaag tgcgtagcgc gccgcgagtg cccccgcaat ggccagcatg aaaaccgcgc
     1981 cgcctagcca aaactgcggc gaggccgtcg ccagcggcac gccgatcacg ttgaaagccg
     2041 cgccatcgtc agcaccgaat tgcacggcgt atgcccactg caccgcgagc acgacggcca
     2101 gcgtccacag caccgccgag gccacgcccc atagataagc cttgctgcac acgcgccagc
     2161 cgtagccaga caggcgtgcc gtgtgccgtt gcgcgaagcc cgccagtcca tccggcgagg
     2221 ccatcacgat cacgatgaag aacagcccca gatagaacgg ccaggcccgc gtcacactcg
     2281 ccaccgcgac gctgaaaaac gtcaggacga tcgcgccggc gaccggcccg aaaaacgccg
     2341 ccgtaccgcc gatcaccgtc gcgatcagca cggcgcccga gcgcatcatg ccgacgcttt
     2401 ccgtcgacac caattcgaca ttgatcagcc ccaaggtgcc tgcaatgccc gcaaaaaacg
     2461 ccgacatcac caccacgcca tagcgcacgc gccgcgggta gcaaccgatc gccgccgcgc
     2521 gcgccggatt gtcgcgcacg gcgttggcca gccgcatgaa cggtgtgcgc gacaacgcga
     2581 acatcgccac gctcgccagc acgcaccaca gcgcgatcag cgcataggct tcgcgtgccg
     2641 ggccgaaact ccagcttgcg agcagcggcc cgctcgcgcg atcgatcggc acgccagcct
     2701 cgccgccgaa ccagtcaggc agcgtccatg cggccgccgc gacgagttcg ccgatgccga
     2761 gcgtgatcat tgcgaacgcc gttccggcgc gccgcgtcga cacgaaaccg aacagcaccc
     2821 cgcacaacgc gccgccgagg ccgccgatca acggcagcag cacgagcggc acgccgatgc
     2881 ggttgaacac ttgcgccgcg atgagcgcgc cgaggccgga ataagcggca tgaccgaacg
     2941 acagcaagcc cgtttcgccg agcagcaggt tgtacgagag cgcaaagacg atcatcgtcg
     3001 cggtctgcgc gagataggcg agcagccagc tttgcggcca caccaagggc ggcacggcga
     3061 ggaacagaac gagcgcgagc cagggtgcga cgattcgcgg catcgagcga gacgtcgcgg
     3121 cggccatcgc gaacgtgtcg ggcggctcac gcatgatcgt ctcgccggcc gaacagtcca
     3181 cgcggacgca gcgccagcat cgcgacaagc agcagatacg gaatcagcgg cgcgaattgc
     3241 gcgagcgtca aggcgtccca ttcggccggc agcgcgcctc cgaatgccca ggtgagatcg
     3301 cccaacgaca cgtcgctcgc caccgcaaag gtctgcacgc agccgacgat caatgacgcc
     3361 gccagcgccc cgctcaacga gcccaagccg ccgatcacga ccaccacgaa cacgatcgac
     3421 cccagcgact ccgccatcgc cggctcgatc acgaagagcg gcgcgccaat cacgccggcc
     3481 agagcggcga gcgccgtgcc cgccgcgaac acgccggtaa acacgcgcgg cacgttgtgg
     3541 ccgagcgcct cgacagcgct cgcatgcgtg agcgcggcgc gcacgatcag ccctgccttc
     3601 gacacgcgca gcaccgcgta gagcaccgcc agcatcgcca gcgacaccgc catcatgaag
     3661 gcccgatagc gtgcgaaggc cgcgccgtag accgtgaaca acggtccgtc gagcgcggcc
     3721 ggaacagtgg cgttaagcgg actcagcccc cagccgagct tgaccagctc gccgagcaga
     3781 tacgcggcgc cgaacgtcag caataattcg ggcagatgac cgtgcacccg cacgcggcgc
     3841 agcagccagc gctccagcgc cgcgccgatc aagccgacga cgagcggcgc gatcaccagc
     3901 gcactccaga aacccgcatg cgccgccacc gaaaagccga catacgcgcc gagcatatag
     3961 aagctcgcgt gcgcgaaatt cagcacgccg agcatgctga aaatcagcgt caaacctgcc
     4021 gacagcatga acaacagcag gccgtaactg acgccgttga gcagattgat gacgaacgac
     4081 tgcacgcgcc tagtccgcct gcatcgcgac ggttagcggc tcgagcgccg cgcgcagctc
     4141 agcgggcagc gtcaccggac gacgcgtgac gcgatccacg tacacgtgca cgaaatgacc
     4201 ttgcgccgcg ggttgatcgt cgccatcctt gaatagaccg acttcgtagc gcacgctcga
     4261 gctaccgagc cgcaccactc gcaagcccgc ttcgacccgc tcgggaaaca ccagcggcgc
     4321 gaagtaattg caatgtgtct cgaccacgag gccgatcgtc gcgccgtgct cgaagtcgag
     4381 cacgccggcg cgaatcagat attcgttcac caccgtgtcg aagtagctgt aatagacgac
     4441 gttgttgacg tggccataga cgtcgttgtc catccagcgt gtggtgatcg gcaaaaaatg
     4501 cgggtaggcg ccgcgcgtgg cgggtgtggg tttgttcatc gtgatgtggt gtagtggatg
     4561 gccaaacgca ttggcgtaac ttactgcccc ggtctttcga caaattcgaa cggcagtccg
     4621 cgccgccgca tccattcgcc gagcgcctgc ccggtgccag cgcgccatgg acgcagtttg
     4681 gccggctcga ccagccggta ttcgagcaat tcgggcgaca gcgccaccgt gccgtacgcc
     4741 ttcacgtgat acgcaatgat cagctcgttc ttgcggatga attcgtacac gccgatcaat
     4801 tcgacggtct ccgcacgcag cgaggtttct tcgagcacct cgcgggcaat gccctcttcc
     4861 ggcgtctcgc cgttctcgag gaagccggtg atcagcgcga acatgccctc aggccatgcc
     4921 gcattgcgcg cgagtaggat cttgccctcg tactcgacga tcgcggccac gaccggcagc
     4981 ggattgttcc agtggacgta gccgcattcg ttgtcgggac agatctggcg gatgcggccg
     5041 ccttcgtgtt cggcatcggc gcgctcgatc aaaggcgtag cgcagcgcgg acaaaattgg
     5101 tattcggtca tggcgagtgg cgcgtctctt atgttttggg tgcggtttta ttcagcggtg
     5161 agggtcgtgc tgtggctggt tcagcatcgg ctgcgcgctg cgcgattcca cttgcccagt
     5221 cgcgcagttg atgcggattc acgccgcgca cagctcgcgc acttccgcgg cgaaacgctc
     5281 gacggtttcc gactgcgtgt cccaggcgca catcagccgg caaccgcccg cgccaatgaa
     5341 ctcgtagaac ttccagccgc gcgcgcgcat cgccttggcg gcctgcggag gcaattgcgc
     5401 aaagaccgcg ttcgattctc gcggaaacat gatgctcacg ccgggaattt cctgcaaccg
     5461 cgtttgcagc aagtcggcca tggcattcgc atgacgcgcg ttgcgcagcc agacgtcgtt
     5521 gtccagcaga ccgagccacg gcgcggagat gaagcgcatt ttcgaggcga gttggccggc
     5581 ctgcttcaaa cggtaagcaa aatcgtcggc gagcgagcgg tcgaaaaaca ccacggcttc
     5641 gccgaccggc aagccgttct tggtgccgcc aaagcacaac acgtcgacgc ccgcgcgcca
     5701 tgtgatctcg gacggatgca cgtcgagcgc cgcgaccgcg ttcgcaaacc gcgcgccgtc
     5761 catgtgcacc ttcagttgac gccgtttggc gatcgccgcg atcgcgcgca cttcttcaac
     5821 ggtgtagacg gtgcccactt ccgtcgattg ggtcagcgtg acaactttcg gcttcgggta
     5881 gtgaatatcg gcgcggcgcg tgacgactgc ttcgattgca ttcggcgtga gcttgccgcc
     5941 gatgcctggc gccgtcagca gcttcgagcc gccggagaaa aattccgggc cgccgcattc
     6001 gtccgtttcg atgtgcgcga gttcatggca gatcaccgag tgatacgact ggcacagcga
     6061 cgccagcgcc agcgaattgg cggccgtgcc gttgaacaca aaaaacactt cgcagtcggt
     6121 ctggaacagg tcccgcagac ggtcgcagac ctggttggtc caggaatcgt cgccgtaggc
     6181 cggttcgtgg ccgctgttat tggc
//
LOCUS       DATASH010000472         9404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2448928,
            whole genome shotgun sequence.
ACCESSION   DATASH010000472 DATASH010000000
VERSION     DATASH010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 9404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9404
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2448928"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="VHV99_13060"
     CDS             complement(<1..1008)
                     /locus_tag="VHV99_13060"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HEX3636399.1"
                     /translation="MRLDFASARFQLSIKEHNMSSDSPAADLESVASTNNGENFSLSE
                     FFRTEATTLLGRVKPFSEYYRETRAKGLAHYGREIVSAVSNRVLVRGIDGTTREMVMF
                     GSNNYLGLTTHPHVLGRIQQALEEFGAGVGGPPLLGGMTQLHKELERRLSIWKGTEDS
                     LLYGSGYQANLGWVTTLLRDNDVLLYDEYNHASLYDGIALTAATTNLKAIRFAHNDTE
                     HLERLLIRVRKGAAQPNRQIFVVVEGVYSMDGDLAPLPRISELCGEYGATLVVDDAHG
                     TGVMGKTGRGTTEHFGLKDSVDICMGSFSKAFGMTGGFLSADREVIDYLRFCSRSYMF
                     SA"
     gene            complement(1250..1381)
                     /locus_tag="VHV99_13065"
     CDS             complement(1250..1381)
                     /locus_tag="VHV99_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636400.1"
                     /translation="MDELTDKLDRQVLRYKERAMTAHSAPTHVLASFDADDAGDYAA"
     gene            complement(1399..2379)
                     /locus_tag="VHV99_13070"
     CDS             complement(1399..2379)
                     /locus_tag="VHV99_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636401.1"
                     /translation="MLSTSVLAAPVAQPLRLLSATPLPAVTGGDFDHFAADLTRNRLY
                     VSAEDFGSIEVFDLPLGGHLASEKNVATQPHKILLSNDGKELFIADAGSASLKIVDTR
                     SFQIEKTIPMKAQPDSGVADDRTGIFYVGNGGVKSHEDHAEVSLISMVDGTLRGNIDV
                     PAGQVKAMAIDRAKDRLFVNFRDRNEIGIIDLKTRKMSGIWKVPGPSRNSSMAFDPAT
                     NRLFVGSRNPGKLYVLDTNDGAVVQALDIVETSDEIIFDTRHRRLYIAGSNGLDVVRQ
                     IDKNHYSVEQHVDTFGGKTAILIPSLERLYVVHTKGPSANEAGLQVFSVQ"
     gene            2369..2710
                     /locus_tag="VHV99_13075"
     CDS             2369..2710
                     /locus_tag="VHV99_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636402.1"
                     /translation="MDSTSEAARLIESNRIFIVGAGGNAANVSPRTLGYHAAGRQKRF
                     DFRKKRVIYNRDLIALLEDVDNVIKMIGYGNFNEAARSAPLVMSELTCNAAQEAGEEG
                     GVIGSAVYCLP"
     gene            3203..3862
                     /locus_tag="VHV99_13080"
     CDS             3203..3862
                     /locus_tag="VHV99_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013690708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3636403.1"
                     /translation="MRILLVESVEQIAWPLASVLRHQGYTVDRVKDDREAQLALASAR
                     YALVVADVLLPRRSGFDFLVHLRRLHRDVPVLTFKEGESAADCVQALDAGADDHLSAP
                     FDTRELIARCRALIRRSQGRSTNQIRHRKLYIDTASRTVTRAGIPVTISDREWAILLW
                     LISYRGIPQSAATLEDCIYGWKEGVESNAIQVHISNLRKKLGADMIETVRRVGYVIRD
                     A"
     gene            complement(4125..5861)
                     /locus_tag="VHV99_13085"
     CDS             complement(4125..5861)
                     /locus_tag="VHV99_13085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636404.1"
                     /translation="MNNTIFRLLPVAAVASMLCACGGSNPGTPTTTSTPPAPTGNKLL
                     VSRSAYDPGFTVAGQLPYNGNPAGTGTAASAVSAVTPGGFPNVFNNDANDANFGVTSE
                     IYLDQWAPGATSASQTTDITTLAKQNGFDISTSFPSKSELALNVSVDKSSITFMGYNA
                     PIDQVDISNSNTPGVIDPTNTDVAAPTYRTVAQLNLAKNALTFTRTNAYSGNNGRAAV
                     LANGTYYMVGNAGNSGSNPNPTYGQLDTLTMNSGLQSIAPGSACAFSQVVGAFQSGNG
                     STYTPVPAGSACSLASMGTISDSLAGQIFSKKTNAGGNQFGFTLASVAGQTFDKTGKD
                     ANLRGLFVGPDGTMYVSKGSGSNGVNTVYQMGAVGALANGGTLPQNAAMAIVRGFPTL
                     NAANLIPSSSTVNPPAWTLSEGVGYPFGMWVPTNNTSLMFVADEGDLGTSAYAPSGSG
                     GLWVYQQSNGTWTPIAHITQGLNLGTAYTVTDTGGTYGASGASYTTTPDGLRNITGQL
                     NSDGSYTIYAVTSTIGNSLGANFDEGADSNQLVTIKLTVNGSTVSTSGFTTLQTAPYG
                     QVLRGVALVPNS"
     gene            complement(6028..7086)
                     /locus_tag="VHV99_13090"
     CDS             complement(6028..7086)
                     /locus_tag="VHV99_13090"
                     /inference="COORDINATES: protein motif:HMM:NF044870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636405.1"
                     /translation="MKKNNILRTLLASGLLSAGLCGAAHAVTTKADAQFEVTKHYTLD
                     GAQRWDYVAYDPVRHHLFVSRDNRVQVLDTHTGKLFGEIENTDGVHGFAFVQDRKLGF
                     VTNGRANTISVVNLDTLKVVDTIPAAGADPDGILYVPSLQRVFVSNGHDKSVSAIDVA
                     TRKVVATTQVGGKPEALAADRQGHVFVNVEDRSEIAELDGRSAAVLAHWPLAPCEKPS
                     GLAIDSVNERLFTVCANHHMMVMDAASGHPVADLPIGGHPDAAAFDSALKLAFSSNGE
                     NGTMTVVHEDDADHYSVVQNLKTLEGAKTMALDPQMHRVYLVSSKFGPAEAATAQNPH
                     PRQTVIPGTFEVLAVQRK"
     gene            complement(7153..7869)
                     /locus_tag="VHV99_13095"
     CDS             complement(7153..7869)
                     /locus_tag="VHV99_13095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX3636406.1"
                     /translation="MATVPASTMQQERTRLYPAAAIALVTEALLMGGAFLLLTHKAPT
                     PVEPPPTLLTLAAPPAPPTTPAPKPAVQTPPKPVVPPPVRPVVPVHHVVTHTPPRQTV
                     PNQPQQAPAPAPVMPSASPSEPAPEPPRPAPPPPPVPAATASPSFEGALRAAIQAALH
                     YPESARMAGMTGRTRVAFKYRDGQVSDVTVVVSSGIGLLDRAAVAAVRDAAYPKPEPA
                     FVGKTLSEQLWVTFNLDDHD"
     gene            complement(7871..8269)
                     /locus_tag="VHV99_13100"
     CDS             complement(7871..8269)
                     /locus_tag="VHV99_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX3636407.1"
                     /translation="MKYFEARKARIEIIPMIDIMFFLLVFFIMITLHMIPNAGLRTQL
                     PSSATTQSLPPPKVTVTLSTDGSLSVDGQALSTSQLTAMLAARPDVAHTSVTIAGSKQ
                     AQIQKLVAVMDACRAAGVTQIALAAQPVAN"
     gene            complement(8272..8961)
                     /locus_tag="VHV99_13105"
     CDS             complement(8272..8961)
                     /locus_tag="VHV99_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX3636408.1"
                     /translation="MNIAHFTDLANDSGGVLYVIAAMLFVALTIIVERFWYLHRVAGS
                     GQVALEQLDHLTALNATTLASCAGQFGSLPTSRLFETAARLAGKASREELGACLDEVI
                     MREAPRIDRFLWVLDTIVTLAPLLGLFGTIMGMFNAFQVLSNPANAPTQVTGGVAEAL
                     IATASGLFVAMIGLVFFNGLHNRVRSIVHQLETVKVALVNRLGVTSHEGSKAVREALL
                     TTVGKLASQGV"
     gene            complement(8984..>9404)
                     /locus_tag="VHV99_13110"
     CDS             complement(8984..>9404)
                     /locus_tag="VHV99_13110"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX3636409.1"
                     /translation="TYYAGHGFSLYGNFTLNSAKQKGTNDWMPNAPRDTAAFGLMYER
                     GPVTGSLITKFIGRQYGLTGNQIPIGGYAVTNLAAGYTFKSPAPWFRDVRLGFQIDNL
                     FNRTSIDWLAGQTVADSTPLYYTVPGRAFIGTMQLDF"
BASE COUNT         1874 a         2750 c         2822 g         1958 t
ORIGIN      
        1 cgccgaaaac atgtatgagc gcgagcagaa cctgaggtaa tcgatcacct cacggtccgc
       61 gctcagaaag cctcctgtca tgccaaaggc tttgctgaag gagcccatgc agatatcgac
      121 gctgtctttc aatccgaaat gttccgtcgt gccacgtcca gttttgccca tgacgccggt
      181 cccgtgcgca tcgtcgacca ccagcgttgc gccgtattcg ccacaaagct cgctgatccg
      241 cggcaacgga gccaggtcgc cgtccatcga gtacacgcct tcgaccacca caaatatctg
      301 acggttgggt tgcgctgcgc ctttccggac gcgaatcagg agccgttcga gatgctcggt
      361 atcgttatgc gcgaagcgga ttgccttgag gttggtcgtc gccgccgtga gagcaatgcc
      421 gtcatagagg ctggcgtggt tgtactcgtc gtaaagcagc acgtcgttgt ccctgagcag
      481 ggtcgtgacc catccgagat tggcctggta gccagagccg tacaacagcg aatcctcagt
      541 gcccttccag atcgacaggc gacgttccaa ctccttatga agttgggtca tcccgccgag
      601 caagggcgga ccgcccacgc ccgcaccaaa ctcctccagc gcctgctgga tgcgcccaag
      661 cacgtgcgga tgcgtagtca ggccgagata attgttcgag ccgaacatga ccatctccct
      721 cgtcgtgccg tcgatccccc tcaccagcac gcggttcgat accgccgaca caatctctct
      781 gccatagtgc gcaagcccct tggcgcgtgt ctcccgatag tactccgaga acggtttgac
      841 tcgccctagc agcgtagtgg cttcagtcct gaagaattca gacagtgaaa aattctcacc
      901 gttattcgta gacgccacag attccagatc tgcagcgggc gaatcagacg acatgttgtg
      961 ttccttgata gaaagctgaa agcgggcaga ggcgaagtct aagcgcaatt cagtttccgg
     1021 gtggtgagtg ccggagtcag aacgggttgg ccgggcagtc gcgcgtcgat atggccaaac
     1081 tcgattttaa atggagttga tcccgcgcgt tcgatggcca agatcgagtc aagttcaaca
     1141 gggactgttc gtgggtgggc acggcaatgt ctggcgtgcg cctgttcgcc gcagtggcaa
     1201 gaaatgcggt cacaccataa ggcaagcact gccgcggcac atcggcatgc taggcggcgt
     1261 aatctccggc atcgtcggca tcgaaacttg cgagcacgtg cgtcggggcg ctatgcgcgg
     1321 tcatcgcacg ctctttatag cgcaggactt gtcgatcgag tttgtccgtg agttcgtcca
     1381 cggtgcctcg ttggaaattc actgaacaga aaacacctga agtcccgcct cgtttgccga
     1441 aggccctttg gtatgaacga catatagtct ttcgagcgat ggaatgagga tggccgtctt
     1501 gccgccgaag gtgtcgacgt gctgttcgac tgaatagtgg tttttgtcga tctgacgcac
     1561 gacgtcgagc ccgttcgacc cggcgatgta gaggcggcga tgccgtgtgt caaaaataat
     1621 ctcgtctgat gtttcgacga tgtccaatgc ctgcacaacc gcgccgtcgt tcgtatctag
     1681 aacgtacagc ttgcccggat tgcgggagcc gacaaacagc cggttcgtgg cgggatcgaa
     1741 cgccattgac gaattgcgac tcggccctgg taccttccag atgccggaca tcttgcgggt
     1801 cttaagatcg ataatcccaa tctcgttcct gtcgcggaaa ttgacgaaca ggcgatcctt
     1861 cgcccggtcg atcgccattg ctttgacctg tcccgccggc acatcgatgt ttcctcgaag
     1921 cgtcccatcg accattgaaa tcaggctgac ctcagcgtga tcctcatgtg atttaactcc
     1981 gccatttccg acataaaaaa tgcccgtcct gtcatcggcg acaccgctat cgggttgtgc
     2041 cttcatggga atcgttttct ctatctgaaa gcttcgagta tcaacgatct tgagactggc
     2101 agacccggca tcggcgatga atagttcctt gccgtcattg ctcagcagga tcttgtgcgg
     2161 ctgagtagcc acgttcttct cactggcgag gtggccacct aaaggaagat cgaagacttc
     2221 aatcgacccg aaatcctcag cagaaacata gagacggtta cgtgtgaggt cagcggcgaa
     2281 gtggtcgaag tcgcctcctg tcactgccgg caagggcgtg gcggatagaa gtcgaagagg
     2341 ttgagcgacg ggcgcggcga gaaccgatgt ggacagcaca agcgaagccg caagactgat
     2401 agaaagtaac cgcattttca tcgtaggtgc cggtggtaat gccgcgaatg taagtccacg
     2461 cacgctcgga tatcacgcgg cggggcgaca gaaacgtttt gactttcgta agaaacgggt
     2521 gatttataac cgggatttaa tcgccttgct ggaagatgtc gataatgtaa taaagatgat
     2581 cggctacggt aatttcaacg aagctgcgcg atctgcgcca ctcgtgatga gcgagctgac
     2641 atgcaatgcc gcgcaagagg cgggagaaga aggaggagtg attggctccg ccgtttattg
     2701 cctgccttaa ggttgcgttc atgcatgtcc cgtagactta cccgcaatct gatgcgcctc
     2761 gagctatcca ctatcagagg aaagccgtgc gtcgcgatgg tcatggggcg gccgcgcttc
     2821 accagttcag aattgctggc atttttgtgc ttgcggctgc gacgcagata acgtaatgga
     2881 tacgtgcaca acgaagatcg ttcgacggca atacagacgt cttcgccacg acctgtcccc
     2941 ctttgcatgt gtcgagcaag acgtgttgtt cccgcatccc cgaggtacct cgatctattc
     3001 gttttcacgc gtttagcgac agcgcctttg ccggtgtgca gataacgaaa aagtaatctt
     3061 gccgtcagcg tatgcatgcg cattggttgc gcacaatctt tacctaacac aggaaacggt
     3121 cgaagactag gagcatccat ctcgttcact tgtactcccg ccgctttgtc tttccctgat
     3181 tcgctcatgc aggtggccgg tcatgcggat tcttctcgtt gaatcagtcg agcagattgc
     3241 gtggccgttg gcgtcggttc tgcgtcatca aggttatacc gtcgatcgag tgaaggacga
     3301 tcgtgaggca cagcttgcgt tagcgtccgc acgctacgca cttgtcgtgg ccgacgttct
     3361 gttgccgcgc cgatctggtt tcgatttcct cgtccactta cgtcgcttac atcgcgacgt
     3421 gccggtcctg accttcaagg aaggcgaaag cgccgcagat tgcgtacaag ctctggatgc
     3481 cggcgcggac gatcatttga gcgccccttt cgacacacgt gaactgattg cgcggtgtcg
     3541 tgcgttgata cgtcgctcgc agggacgaag caccaatcag atccggcatc gaaagctgta
     3601 tatcgacacc gcgtcgcgaa ctgtgacgcg cgcaggcatt ccagtcacga tatccgaccg
     3661 tgaatgggcc atcctgcttt ggctcatttc atatcgcggc attcctcagt cggcggccac
     3721 gctcgaagat tgcatttacg ggtggaagga aggagtggaa agtaacgcaa ttcaggttca
     3781 catctcgaac cttcgcaaga agctcggcgc cgatatgatc gaaacggtgc gtcgggtggg
     3841 atatgtgatc cgcgacgcgt agtttgaaga gaacggtgac ttttccgtga ataaccacgc
     3901 gccacaggcg acggattgac ggaccgctac cggatagcgt ggcgtcttca gcttgccata
     3961 agctaaggtc actggaatgg cgcctggcgc gagaatcagt tttccttcag gttttgtaag
     4021 tggctctaag ccacgtcgca cgtgcaatcc ggcctcgcaa ataatttgac cccgtccgaa
     4081 ggacggggcc gctgtaccta tgccagctta gaggtatccg aagcttacga gttaggcacg
     4141 agcgcgacgc cgcgcagcac ctgaccgtac ggtgcagtct gcagcgtggt gaagccgctc
     4201 gtcgagactg tcgagccatt caccgtcagc ttgatcgtga ccagctggtt cgaatcagca
     4261 ccttcgtcga agttcgcacc gagactattg ccgatcgtcg atgtcactgc gtagatcgtg
     4321 tacgaaccgt cgctattcag ctggcccgtg atgttgcgca ggccgtccgg cgtagtcgtg
     4381 tagcttgcgc ctgaagcgcc gtaggtgccg cccgtgtcgg tgaccgtgta ggccgtgccg
     4441 aggttcaggc cttgcgtgat atgcgcgatc ggcgtccacg taccgttcga ttgctgatag
     4501 acccacagac cgccgctgcc gctcggcgcg tatgcgctgg tacccagatc gccctcatcg
     4561 gccacgaaca tcagcgaggt gttgttggtc ggaacccaca tgccgaacgg atagccaacg
     4621 ccctcggaga gcgtccacgc cggcgggttg accgtggacg aggacggaat cagattcgcc
     4681 gcgttcaaag tcggaaagcc ccgcacgatc gccatcgccg cgttctgcgg gagtgtgccg
     4741 ccgttcgcga gcgcgccgac agcgcccatc tgatagaccg tgttcacgcc gttgctgccg
     4801 ctgcccttcg acacgtacat cgtaccgtcc gggccgacga acaggccacg caggttggcg
     4861 tccttaccgg tcttgtcgaa cgtctggccc gccaccgaag cgagcgtgaa accgaactga
     4921 ttaccgcctg cgttggtctt cttactgaag atctggccgg ccagcgagtc cgagatcgtg
     4981 cccatgctcg cgagcgaaca tgcggatcca gccggcaccg gcgtataggt gctgccattg
     5041 cccgactgga acgcgccgac cacctgcgag aacgcgcagg cgctgcccgg cgcgatgctc
     5101 tgcaaaccgc tgttcatcgt cagcgtatcg agctggccgt aggtcgggtt cggattcgag
     5161 cccgagttgc cggcgttgcc aaccatgtag tacgtgccgt tggcgagcac agccgcgcgg
     5221 ccgttgttgc ctgaatatgc gttggttcgc gtgaacgtca gcgcgttttt cgcgaggttg
     5281 agctgggcaa ccgtgcgata ggtcggtgca gctacgtcgg tattggtcgg atcgattacg
     5341 cctggcgtat tcgagttcga gatgtcgacc tggtcgatcg gtgcgttgta acccatgaac
     5401 gtgatcgagc tcttgtcgac cgacacgttc agcgcgagtt cggacttcga cggaaagctg
     5461 gtcgaaatat cgaagccgtt ctgtttcgcc agcgtggtga tgtccgtggt ctgcgacgcg
     5521 ctggtcgcgc ccggcgccca ctggtcgaga tagatctcgg acgtcacgcc gaagttggca
     5581 tcgttggcat cattgttaaa gacattcggg aaaccgcccg gcgtcactgc gctgacggcc
     5641 gatgcagccg tgcccgtgcc cgccgggttg ccgttgtacg gcaactggcc cgcgactgtg
     5701 aaacccggat cgtaggccga acgcgacaca agcagcttat tgccggtagg ggcggggggc
     5761 gtgctcgtcg tggtaggcgt cccgggattg gaaccgccgc acgcgcatag catcgatgcg
     5821 acagcagcga ccggcagcaa tcggaaaatc gtgttgttca tgctatttcc tttgtttgtt
     5881 atttagataa acccatgatg tgttgcttta acagatactg cgaccaaccc aaaaccatcg
     5941 agcgttgcga aaacgcgcac ttcttttgaa atcgggagcg gctgacgcgc gaaagcagcg
     6001 cgcgtacaag ccacctatgg accgcgctta cttacgttgc acggcgagca cttcgaacgt
     6061 gccgggaatg accgtctgac gcggatgcgg attttgcgcc gtcgccgctt cagcaggacc
     6121 gaacttcgaa gacaccagat acacgcggtg catttgcgga tcgagcgcca tcgtcttcgc
     6181 gccttccagc gtcttcaggt tttgtacgac gctgtagtgg tccgcgtcgt cttcgtgcac
     6241 caccgtcatc gtgccgttct cgccattcga actgaatgcc agtttcaagg ctgaatcgaa
     6301 cgcagcggca tctggatgac cgccaatcgg taagtccgct accggatggc ccgacgcagc
     6361 atccatcacc atcatgtgat ggttggcaca tacggtgaaa aggcgctcgt tcacgctgtc
     6421 gatggcgagc ccgctcggct tctcgcacgg cgcgagcggc caatgcgcca gcacggctgc
     6481 ggacctgcca tcgagttccg caatctcgct ccggtcttcc acgttgacga agacgtggcc
     6541 ctgacggtcg gccgcgagcg cttccggctt gcccccaacc tgggtggtag caaccacctt
     6601 gcgtgtcgca acatcaatcg cgctcacgct cttgtcgtgc ccattgctga caaaaacacg
     6661 ctgcagggag ggcacataca aaatgccgtc cggatccgcg ccggcggcag gaatggtgtc
     6721 gacgaccttc agcgtatcga ggttgacgac actaatcgta ttcgcgcggc cgttcgtcac
     6781 gaagccgagc ttgcgatcct gcacgaacgc gaaaccgtgt acgccgtcgg tgttttcgat
     6841 ttcaccgaac agttttcccg tgtgcgtatc gagcacctga acacggttat cgcggctcac
     6901 gaatagatga tggcgcaccg ggtcgtacgc cacgtagtcc catcgttgcg cgccgtccag
     6961 cgtatagtgc ttcgtcactt cgaactgcgc gtcggccttc gtggttaccg catgcgccgc
     7021 gccacacaac ccggcggaaa ggagtccgct ggcgagtaac gttcgcagaa tgttgttctt
     7081 tttcacgttg aaataactcc ggttgattca gtcaatgctg ttgatccgat cgttaggcga
     7141 ggtcgttgac gatcaatcgt gatcgtcgag attgaacgtg acccacaact gttcagaaag
     7201 cgttttgccc acaaaggccg gctccggctt tgggtaagct gcgtcgcgca ccgccgcgac
     7261 agcggcgcga tccagcaatc caattccgct cgagaccacc accgtcacat ccgacacctg
     7321 accgtcgcga tacttgaagg ccacccgcgt gcggccggtc atgccagcca tgcgtgccga
     7381 ctccgggtaa tgcagtgccg cctgaattgc ggcacgcaat gcgccttcga aactcggcga
     7441 cgccgttgcg gccggaaccg gcgggggcgg cggggcaggg cgcggcggtt cgggcgcagg
     7501 ctcggacggc gaggccgaag gcatcaccgg tgccggcgca ggagcctgct gaggttgatt
     7561 cggaaccgtt tgcctgggcg gcgtatgtgt caccacgtga tgcaccggca cgactggccg
     7621 aaccggcggc ggcaccacgg gcttgggcgg tgtttgcacc gctggctttg gcgcgggcgt
     7681 cgtcggcggt gcggggggcg ccgcgagcgt caacagcgtc ggtggcggtt ccaccggcgt
     7741 gggcgccttg tgcgtcaaca gcaaaaacgc accgcccatc aggagcgctt ccgtaactag
     7801 cgcgattgct gcggccggat acaggcgcgt tcgttcttgc tgcatcgtgg aagcgggaac
     7861 agttgccatg tcagttcgcc acgggttgcg cagccagcgc gatctgcgtg acgcccgcgg
     7921 cccggcatgc gtccatgacc gcgacgagct tctggatctg cgcctgcttc gagcccgcaa
     7981 ttgtcaccga cgtatgggcc acgtcggggc gcgcagcgag catcgccgtc aactgcgagg
     8041 tgctgagcgc ctggccgtcg accgacagcg agccgtcggt cgacagcgtg accgtcactt
     8101 tgggcggcgg tagcgactgc gtggtggcgc tcgatggcaa ttgcgtgcgc agaccggcat
     8161 tcgggatcat gtgcagcgta atcatgatga agaacaccag tagaaaaaac atgatgtcga
     8221 tcatcggaat gatctcgatg cgagccttgc gcgcttcgaa gtacttcatc gttacactcc
     8281 ctgcgacgcg agcttgccga ccgtcgtgag caacgcctca cgtacggctt tcgagccttc
     8341 atgggaagtg acccccagcc ggttgacaag tgcgaccttc accgtctcca gttgatgcac
     8401 gatcgagcgc acgcgattgt gcaagccgtt gaagaacaca agaccgatca tcgccacgaa
     8461 cagacccgac gccgtcgcga tcagcgcttc ggcgacgccg cccgtcacct gggtcggcgc
     8521 attggcggga ttcgacagca cctgaaacgc gttgaacatg cccatgatcg tgccgaacaa
     8581 ccccagcaac ggggcgagcg tcacgatcgt gtcaagtacc cacagaaagc ggtcgatgcg
     8641 cggtgcttcg cgcatgatca cttcgtcgag acatgcgccg agttcctcac gggatgcctt
     8701 gccggccaac cttgccgcgg tctcaaacag cctgctggtg ggcagactgc cgaactgccc
     8761 ggcgcacgac gcgagcgtcg tcgcgttcaa cgcagtcagg tggtcgagtt gttccagcgc
     8821 aacctgaccg ctgcccgcca cgcggtgcaa ataccagaag cgttcgacga tgattgtcaa
     8881 cgcaacgaac agcatcgcgg cgatgacata gagcacgccg cccgaatcgt ttgcaaggtc
     8941 ggtgaagtgg gcaatgttca tgataaaaat ttcctggaaa agatcagaag tccagctgca
     9001 tcgtgccgat gaacgcacgg cccgggactg tgtagtagag cggcgtgctg tcagcaacgg
     9061 tttggccggc gagccaatcg atgctggtcc ggttaaagag attgtcgatc tggaagccaa
     9121 ggcgtacgtc gcggaaccac ggagcggggc tcttgaacgt atagccggcg gcgaggttcg
     9181 tcacggcata gccgccaatc gggatctggt tgccggtgag gccgtattgc cggccaatga
     9241 acttggtgat cagcgaacct gtcaccggtc cgcgttcata catcagaccg aatgcggccg
     9301 tgtcgcgcgg cgcgttcggc atccagtcgt tggtgccctt ctgcttggcc gagttgagcg
     9361 tgaagttgcc gtacaggctg aagccgtgcc ccgcgtaata cgtc
//
LOCUS       DATASH010000473         2169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2052377,
            whole genome shotgun sequence.
ACCESSION   DATASH010000473 DATASH010000000
VERSION     DATASH010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2052377"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            859..1414
                     /locus_tag="VHV99_13115"
                     /pseudo
     CDS             859..1414
                     /locus_tag="VHV99_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006046732.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            1473..2169
                     /locus_tag="VHV99_13120"
                     /pseudo
     CDS             1473..2169
                     /locus_tag="VHV99_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884056.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
BASE COUNT          403 a          647 c          645 g          474 t
ORIGIN      
        1 cggcgactga ttgcgccctt catccttacg ctccggcagt ttgcttttct cattgtcact
       61 gtgggcggcg ggctggggat gttcgatatc ggcaggcttg gtcgattcgg cttccccggt
      121 ttctttcgtt tcgatcatgg cgtactcctg gtttgagaac atccatgacg cagcaaggca
      181 cggacctgat gaggctattt ccacgcttcg gcctgcggct ggtaggcgcc aaaagcctac
      241 gggttgcact cgcgcggtgg cgcgccgctt cgcgcgcgtt caagatccgg ggcgcctgcg
      301 ctgagtgacg gcgtatcaaa aggcttgaat agccttgtca tggccgcttt accgcaactg
      361 gcacaacatt cgcttaacac gagaccagag tagaagcggc tagcgaagcc ggcatgcata
      421 ggctggctat cgtggggcat tgctcttggg gcgcatcgtg cgcccacgtc gaagcaattc
      481 ggcgtttgaa cggaaccgtc cggttccgcc gacaaggagc gtgcgatgca gttgccccaa
      541 tcattcacac gactgttgtt gttgttgttg ttgtgcgccc tcgattgcgc ctttggtttc
      601 gcgacttttg ctgtctcgcg aggccgacct tgtgcgtccg taggtcacgg atgtcgcata
      661 cgttacgtgc ttggcatggg tcgcgtcgac gctgcctgtc gttttgcctg tccaccacgc
      721 cccaagttcc cacggagttc agtcacgcgc ggtctgcgtc atcttttccg tattgcccga
      781 gttgcttttt tcggctgctc gcttcgctcc tcatcgggtt catcgcttgc gcgcgaggcc
      841 ggcgtcgttg cactcgccat tgtcacgttc gctgcgccgt cggcgcacgc cgacgatcac
      901 gtgaccttgc tgacgagctg gtatgcgcaa gccgaacatg gtggcttcta tcaggccgtt
      961 gcgacgggta tctacaagaa gtacgggctg gatgccacga tcagaatggg tggtccgcaa
     1021 gtcaacggta tgcaactgct cgctggaggc caggcgcatt tcctgatggg ccacgacttc
     1081 caggtgctct ccagcgtcga ggccggcatt cccgtgacaa cggtcgccgc ggctttccag
     1141 ttcgacccga tccggcgccg gctaacgcgc cgatcaaaaa agacaagccg cagatgagcg
     1201 acgcgcaact cgcgtatggc gtcgcccaac tcaggaagct caagctcgtg acgggcggcg
     1261 atgcagccac gcaggacatt ggcacgatga ccgacgcgcg cgctggaaga agaccttcga
     1321 ttacatggtc ggtgcgacgc tgctcaaggc ctcgtccgac tatcgcgcgg cctacacgct
     1381 gcaatacatc cagaacgcga aggtcatgcc ctaagggttc cttaacgcat aagccgctta
     1441 catgcctgaa cgcttaaaga aggagtttcg ccatgctggt cacccgtcaa aaagttttgc
     1501 gccgcttctg gtacgcaatc atgccgatgg cgcaactcga tgccggtccg cagtcgttca
     1561 ccttgctcgg tgagccgatc gtgttatgga agggcgcagg cggaatgccg catgcgttgc
     1621 gcgatcgttg ttgccatcgc acggcaagac tgtcgaaagg ttttgtcgac ggcgacggca
     1681 atttcgcctg cggttatcac ggctggacct acgactgcac gggtcaatgc gtgaagattc
     1741 cgccgaacaa cggcggcgcg atcccgtcgc gcgcgatcgt gccttcgtat tgccgcgagg
     1801 aacgctacgg ctacggatgg gttgcgctcg acgatccgct gcaatcgatt cctgagtttc
     1861 cagaagaaag gcgcacccgg ctgccgccgc atcctgcagt tctacgagcg ctgggaaacg
     1921 agcccactcc gcatgatgga aaactcgttc gacaactcgc atttcagctt cgtgcacaag
     1981 ggcaatttcg gcttgttcga caacccgaaa ccgtcgaaat atgaatttcg tccgaacgag
     2041 tggggcttcg aagcagagac gcatgtgccg gtacgcaatc ctgaagcaag ctatcggatc
     2101 accggtacga ccgaggaagt caccgagcgt catctgatca accgctggta catgccgttc
     2161 gcgcgccgc
//
LOCUS       DATASH010000474         4689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6798973,
            whole genome shotgun sequence.
ACCESSION   DATASH010000474 DATASH010000000
VERSION     DATASH010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4689
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6798973"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VHV99_13125"
     CDS             <1..556
                     /locus_tag="VHV99_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3636410.1"
                     /translation="PCFGQTIAMRRTTLEKIGGFEPFVRHLAEDHAIGEAVRLAGEKV
                     SIPPFTVTHACVESSARQLIAHELRWSRTIRSVDPLGHLGSVLAHPLAFALLAVMLSG
                     AAAWSWQLVLATMGARLALELLSDRVLRQAHRDLWLLPLWDIVSFAVFVASFWSSRVT
                     WRGFGFEVDSDGLLSAVQDERVID"
     gene            618..1613
                     /locus_tag="VHV99_13130"
     CDS             618..1613
                     /locus_tag="VHV99_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018417791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HEX3636411.1"
                     /translation="MMKHFGRVAALVGLIGSLWLVWHDNPVTVLHALRTAGSGLLLAA
                     LAHVLPMLANACDWRTLIRRGHRPSMLTMLHLVWVRESVNCMLPVARVGGEMVSYRML
                     RLRGVRASTAAASLIVDMQLTVISHLLFTMVGIGFLLAHAQSGAVRFAGQLAWGVAVL
                     APLLVLFALVQHASPFERVTRALNRITSGKLAALIGRSAQIDQTIKLIWRRRGVVLRY
                     LFFWQPLQCVLTSLEIWLALYFLGARISLLEAVVIESLIQAVSSAAFFVPGGLGVQEG
                     GFVLIGGALGLDPSTCLALAGARRIRDLLIFIPGLLAWQRAESSNWLAARPTRPG"
     gene            complement(1643..1855)
                     /locus_tag="VHV99_13135"
     CDS             complement(1643..1855)
                     /locus_tag="VHV99_13135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636412.1"
                     /translation="MTSETGTADGSVIYAVDDDQAIRFTLDALPRSHFRCFVTASLSK
                     DLLSPDDRVSMELQPTVDIMRQNACG"
     gene            1912..2178
                     /gene="hfq"
                     /locus_tag="VHV99_13140"
     CDS             1912..2178
                     /gene="hfq"
                     /locus_tag="VHV99_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018574547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEX3636413.1"
                     /translation="MSNQASVQDEFLQSLLEEKTSASVYLINGIRLSGQVASFDSYVV
                     LLKSVSGHQMIFKHAISTVMPNTGGGMRRPISNAEDNQAETSRA"
     gene            2625..2912
                     /locus_tag="VHV99_13145"
     CDS             2625..2912
                     /locus_tag="VHV99_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007580626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636414.1"
                     /translation="MRVVYLGWFLQRAGYGNCPAEQFKVVEYAVEATLAHAHECGEWR
                     LPDEMIVDFEALLALYDSQLASAPLHEVLAAEQKLRAFLAGTSSSPIPVNA"
     gene            complement(3006..3344)
                     /locus_tag="VHV99_13150"
                     /pseudo
     CDS             complement(3006..3344)
                     /locus_tag="VHV99_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007580625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            3645..>4689
                     /locus_tag="VHV99_13155"
     CDS             3645..>4689
                     /locus_tag="VHV99_13155"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX3636415.1"
                     /translation="MTGIIGVLSVNIAESVHLAEIFGDEAARQAMADFSEAADGLLTR
                     LLAQHAVIDRYGSAADGRWAACFRVLSDGLQRDAREVAEAIEHAGRNLIRESLLAVFG
                     GATGARITANLSVFLMSPETGERDAKTCWTQWTEDRLRSQPQRQVVEQIGPSTDVTAI
                     LTHRCIQTFLQPIVRVSDRAVVGFEALSRGPKGSPLERPDKLFDAAYAAGRTVDAELL
                     CAELALERTKDKLPPGAFLTINLGPDALALAADKLALAGRPEVMIELTEHLPLNEAEG
                     LVEVVDRLRTLGIRLALDDTGCGFANFDTVRLLRPDIVKLCITVIRNADKGSHFMAAI
                     RESAERLCKLGCLV"
BASE COUNT          842 a         1339 c         1508 g         1000 t
ORIGIN      
        1 gccatgcttc gggcagacca tcgcgatgcg acgcactacg ctcgaaaaga ttggcggctt
       61 tgagccattc gtcaggcatc tggccgagga tcatgcgatc ggtgaggcgg tgcggctcgc
      121 cggcgaaaag gtgtcgattc cgcccttcac ggtcacgcat gcctgcgttg aaagcagtgc
      181 gcgccagctg atcgcgcacg agctgcgctg gagtcgcacg atccgcagtg tcgatccgct
      241 cggccatcta gggtcggtgc tcgctcaccc gctggcattc gcactgcttg ccgtgatgtt
      301 atccggcgcc gccgcgtggt cgtggcaact cgtgcttgcg acaatgggcg caaggctcgc
      361 gcttgagctg ttgtcggacc gcgttttgcg ccaggcacat cgcgacctat ggctgttgcc
      421 gctgtgggat atcgtttcct ttgcggtatt cgtcgcgagt ttctggtcgt cgcgtgtgac
      481 gtggcgcggc ttcggttttg aagtcgacag tgacggcctg ttgtcggccg ttcaggacga
      541 acgggtaatc gactgatgaa gcaaaagcga ttcgcgcgca cgagcggcgc tgcgcggcac
      601 cactccacgg cactggcatg atgaagcatt ttggccgcgt cgcggcactc gtcggcctga
      661 ttgggtcgct gtggctcgtc tggcacgaca atcccgtcac cgtgctccac gcgctgcgaa
      721 ccgccggctc cggcttgctg ctggcagcac tggcgcacgt gctgccgatg ctcgcgaacg
      781 cgtgcgactg gcgcacactg atccgccgcg ggcatcgtcc ctccatgctg acgatgctgc
      841 atctggtgtg ggtgcgcgaa tcggtgaatt gcatgctgcc ggtcgcgcgc gtcggcggcg
      901 agatggtgtc gtaccggatg ctgcggctcc ggggcgtgcg ggcgtcgacg gccgcagcca
      961 gtctgatcgt cgatatgcaa ctgacggtga tcagccattt gctattcacg atggtcggga
     1021 tcggttttct gctcgcgcat gcgcaatccg gcgcggtacg gtttgccggc caactggcgt
     1081 ggggcgtggc ggtcctcgcg ccgctgctgg tgctgttcgc gctggttcaa catgcgagtc
     1141 cgttcgaacg ggtcacgcgc gcgctcaacc ggatcacgag tggcaagctc gctgcgctca
     1201 tcgggcggtc ggcgcagatc gatcagacga tcaagctgat ctggcgtcgg cgcggtgtcg
     1261 tgttgcgata cctgttcttc tggcagccgc tccaatgtgt gctgacttcg ctggaaatct
     1321 ggctcgcgct ctacttcctc ggcgcgcgaa tcagcctgct cgaagcggtg gtgatcgaat
     1381 cgctgattca ggcggtgagt agcgcggcgt ttttcgtgcc cggcggcctt ggtgtgcagg
     1441 agggcggctt cgtcctgatt ggcggcgcgc ttggcctcga tccgtcgaca tgtctcgcgc
     1501 ttgccggcgc gagacggatt cgcgatctgc tgattttcat tcctggactt cttgcgtggc
     1561 agcgtgccga atcatcgaac tggcttgccg cacgcccaac tcgtccaggc tgagccggcc
     1621 cttggaccag tattgataag tgttatccgc atgcgttctg gcgcatgata tcaaccgtcg
     1681 gttgcagttc catcgatacc cggtcgtctg gcgaaagaag gtctttcgag aggctggcgg
     1741 tgacgaagca acgaaaatgg gagcgcggaa gcgcgtcgag cgtaaaccga attgcctggt
     1801 cgtcatcgac ggcgtaaatg acggaaccgt cggctgttcc ggtttcagaa gtcattcact
     1861 gaaggtgtaa gcgtacaatg acccggcgtg cgaccgacgg cggagaacaa catgtcaaat
     1921 caggcctcag ttcaagatga attcctgcag tcactactcg aggaaaagac ttccgcaagc
     1981 gtttacctga ttaacggaat cagattgagt ggtcaggtcg cgtcgttcga ctcgtacgta
     2041 gttctcctga agtctgtttc cggtcaccag atgatattca agcacgcaat ctctactgtg
     2101 atgccaaaca cagggggtgg gatgcgaagg cctatttcca atgcggaaga caatcaggcg
     2161 gaaacttcgc gtgcgtagtg cacgagccct gcgtacaatt gcagccgcat cgtgcggtgc
     2221 accagggcag gcgtgaaagc cgccggtttc cgtatccggc tggatgcgcc cggcgacagg
     2281 gtcgaaggtg tcgcccccgg acgggcgtgc tatccacgca cgtgacgttg ccccgaccac
     2341 aacccctgcg cctggatagg tgcaaaggga ttgaaatgcg aagtcaagtc ccggcttgac
     2401 gcaattgagc tgaggacgcg ggtttcaccg gtacaggtcc ccgttgtcga ccgcggctac
     2461 aagggtgaaa taagtcgcgc agtacccgcg tccataaacc gctgaccagg gcgcttctgt
     2521 tgccgatgga tcgggcgtcg gcgaatgcac agtctctcgc cagtcatctt gcgctggtcg
     2581 catgccggag tggtcacgga aacggacacc atgtcaatga actcatgcga gtggtgtatc
     2641 tgggctggtt tctgcagcga gccggatacg gcaactgtcc ggccgagcaa ttcaaggttg
     2701 tcgagtatgc cgttgaagcg accctggccc atgctcacga atgtggcgag tggcggctac
     2761 cagacgaaat gatcgtcgat ttcgaggcac ttctcgctct ttacgattcg caactcgcca
     2821 gtgcgccact gcacgaagta ctcgcggcgg agcagaagct cagggcgttt ctcgccggga
     2881 cgtccagttc cccaataccc gtaaatgcct gactctggcg tggacagtgc gcggccgtcc
     2941 ggcgttatgc ggcggtcggt ctgtggctga acgcagtcgc cgggcctggt cgttcttgtc
     3001 cgggttcatg cgtcgtcctc cgcaatgccg ggttccgggg taggccgcgc ttcccagttc
     3061 atgcggtggc gtttcaccgt ttcgcgatgg cgtttgtcct cttcctcgtg caggtaaagg
     3121 ctggtggtgt tcagcgacac gtgaccgaga ttgtcgcgca cggtgcgcag gtcggcaccc
     3181 ccgtcggcca ggtgcgagcc cgatgtatgt cgcagcccgt gcgccgatgc acggtccagc
     3241 tcgtcggcgc gatccgtgaa ctcaggtccg cgggcgcgca gccacgcggc ggcgttgccg
     3301 aatacgcttt tgatcgcgtc atgctttgac ggtcggggca gacatgacaa agatgcaatc
     3361 ttgacgaccg gaccatgtca tgtcggtgaa ggcacactga tcaagtatcg cgcagcagca
     3421 ggcttcaaaa cagaaatggg atgctgacta tccctgggtc ggcttaggct ctctgccgtt
     3481 tgcccgaaat tgtcgtctga tgcaacacac aacagcttta ccgcattcgg atcatacagg
     3541 ccatgcaggc aacggatcgg atactcgccc aatctaggaa tagtgtggta gatgcaaatg
     3601 aagtcgctga agcgtacgtg ccgcggcggt cagcgttgtc gcaaatgaca gggatcattg
     3661 gcgtcctgag cgtgaacatt gcggagagcg tgcacctggc tgaaatcttc ggtgatgaag
     3721 cagcccgcca ggcgatggcc gattttagcg aagcagcgga tggcttgctc acgcgtttgc
     3781 tggctcaaca tgcggtgatt gaccgatatg gttctgctgc cgatggaagg tgggcggcgt
     3841 gctttcgtgt cctttcggac ggcctacagc gagatgcgag ggaagtggcg gaggccatag
     3901 agcacgcggg gcgcaacctg atccgggagt cactgctcgc tgtattcggc ggcgcgacgg
     3961 gcgctcgcat aaccgccaac ttgtccgtat ttctcatgtc ccccgaaaca ggtgagcggg
     4021 atgcaaagac atgctggact caatggaccg aagaccggtt gaggtctcaa ccccaacgtc
     4081 aggttgtgga gcagatagga ccttctaccg acgtgacagc gattctgacg caccgctgca
     4141 tacagacatt tctgcagccg atcgtgcgcg tcagcgatcg tgcggtggtt ggcttcgaag
     4201 ccctttcccg aggtccgaag gggtcacctc ttgaacgccc ggataagttg tttgatgcgg
     4261 cctacgccgc cgggcgcacc gtggatgcgg aattgttgtg tgccgaattg gctctcgagc
     4321 gcactaaaga caaattaccg ccaggcgcct tcctgaccat caatttgggc cctgatgcct
     4381 tggcccttgc ggcggacaag ctggcgttag ccggcagacc ggaagtgatg atcgaactca
     4441 ctgagcacct gccgctcaac gaagcagagg gtttggtgga agtggtcgat cgtttgcgca
     4501 ccctggggat tcggttggct ctggatgaca caggatgcgg gttcgccaac ttcgacaccg
     4561 tccgcctgtt gcggccggac atcgtcaaac tttgcatcac ggtgatacgc aacgccgaca
     4621 agggctcgca ctttatggcc gcgattcgtg agtcagccga gcggctctgc aagctgggtt
     4681 gcctggtgc
//
LOCUS       DATASH010000475         3535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3241025,
            whole genome shotgun sequence.
ACCESSION   DATASH010000475 DATASH010000000
VERSION     DATASH010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3241025"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="VHV99_13160"
     CDS             <1..567
                     /locus_tag="VHV99_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX3636416.1"
                     /translation="CLAALYESWSIPFSVIMVVPLGVIGALLAATLRGLENDVFFQVG
                     LLTTVGLSAKNAILIVEFARELQQGEGMGPIEAALEAARLRLRPILMTSLAFILGVMP
                     LAISNGAGSASQHAIGTGVIGGMLTATFLAIFMIPMFFVVIRAKFGGEKEDPDEALEH
                     YNQHHPHDPQGGGSAGGSTDQGSGKDGH"
     gene            569..2116
                     /locus_tag="VHV99_13165"
     CDS             569..2116
                     /locus_tag="VHV99_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEX3636417.1"
                     /translation="MQKHSLIAVAVALFAAGCTMAPKYQRPAEPVTSTFPTGGVYETQ
                     PGATQPGPGETGMSTAARSAGGQAAVDIGWRDFFVDPRMQRIIEIALKNNRDLRVSVL
                     NMQASAAQFRIVRAGLFPTLDAAVSQSNQRTPKDLSFFNQTITNSYSVGLNASWEIDF
                     FGRIQSLKDQALAQYLATAQARKAAEISLVSQVANQYLTVLELDDLLKVTQNTLKTSE
                     ESYRIAKLQFDNGTGSELDLRQSQTVVETASANLQSQDRLRAQAENALVLLVGEPLPA
                     DLPPGLTLNDQNLLTDIPAGLPSDLLTRRPDIMEAEENLLAANANIGAARAAFFPKVS
                     LTGSFGTLSPSLGGLFKPGSAAWSFAPSITLPIFEGGQNLANLDLANIQKKVQIATYE
                     KAIQTAFREVADGLAARGTYDQQIQALERDTFAEQRRLDLSNLRYTNGVDSYLSVLTA
                     QTDLYAAQLLLTTARMERLQNLVTLYQALGGGWIERNGEQPRPADAPVDYGAASAPVA
                     ASAATAG"
     gene            complement(2256..2426)
                     /locus_tag="VHV99_13170"
     CDS             complement(2256..2426)
                     /locus_tag="VHV99_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636418.1"
                     /translation="MLEVLEVLEVLEVLEVLEVLEVLEVREVLEVLEVREVLECLRSA
                     RVAEVTRIQRTK"
     gene            complement(2465..>3535)
                     /locus_tag="VHV99_13175"
     CDS             complement(2465..>3535)
                     /locus_tag="VHV99_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HEX3636419.1"
                     /translation="AAGVIGILLAPAVGKLLRFFPPVVIGVVISVIGLSLMEVGINWA
                     AGGVGNPDYGNPIYLGLSLVVLMLILLINKFAKGFIANISVLLGIVAGFVIALALGRV
                     NMEGVTHAPWVGFVMPFHFGLPHFDPLAIATMVTVMFVTFIESTGMFLAVGDMVDRPV
                     DQKTLVRGLRVDGLGTLIGGIFNSFPHTSFSQNVGLIGVTGVKSRFVCAMGGVILVLL
                     GLFPKMAQVVASVPAFVLGGAGIVMFGMVAANGIKVLAKVDFVKNHHNLFIVAVSIGM
                     GLVPVVSPQFFSKLPPALSPLLHSGILLASVSAVVLNLIFNGVKSEKKAQCEIRKAGH
                     DFDGRGGNEPPRDEMLRAIDLQ"
BASE COUNT          710 a         1121 c         1092 g          612 t
ORIGIN      
        1 tgtctcgcgg cgctgtatga aagctggtcg atcccgttct cggtgatcat ggtggtgccg
       61 ctcggcgtga tcggcgcact gctggccgct acgctgcgcg ggctcgagaa cgacgtgttc
      121 ttccaggtgg gtctgttgac cacggtgggg ctgtcggcga agaacgcgat tctgatcgtg
      181 gaattcgccc gcgagttgca gcagggtgaa ggaatggggc cgatcgaggc cgcgctcgaa
      241 gcagcgcgtc tgcggttgcg tccgattctg atgacctcgc tcgcgttcat tctcggcgtg
      301 atgccgcttg cgatcagtaa cggcgccggc tcggcgagcc agcacgcgat cggtaccggc
      361 gtgatcggcg ggatgttgac ggcgaccttc ctcgcgattt tcatgatccc gatgttcttc
      421 gtcgtgatcc gcgcgaagtt cggcggcgag aaggaagacc cggatgaggc gctcgaacac
      481 tataaccagc accatccgca tgatccgcaa ggtggcgggt cggccggcgg gtcgactgat
      541 caaggctcgg gcaaggacgg acattgagat gcaaaaacac tctttgattg cagtggcggt
      601 tgcgctgttc gccgcgggtt gcacgatggc gccgaagtac cagcgcccgg ccgaacccgt
      661 gacgagcacc ttcccgaccg gcggcgtgta tgaaacgcag ccgggcgcga cccaaccggg
      721 cccgggggag acgggcatgt cgaccgcggc gcgcagcgcc ggcggtcagg cggcggtcga
      781 tatcggctgg cgtgatttct tcgtcgatcc gcgcatgcag cggatcatcg agatcgcgct
      841 gaagaacaac cgcgatctgc gtgtgtcggt gttgaacatg caggcgtcgg cggcgcagtt
      901 ccgcatcgtg cgtgcggggc tgttcccgac gctcgacgcc gcggtttcgc aaagcaacca
      961 gcgcacgccg aaagacctgt cgttctttaa ccagacgatt accaactcgt attcggtcgg
     1021 cctgaacgcc tcgtgggaaa tcgacttctt cgggcggatt caaagcctga aggaccaggc
     1081 gttggcgcag tacctggcaa cggctcaggc gcgcaaggcg gcggaaatct cgctggtctc
     1141 gcaggtggcg aatcagtatc tgacggtgct ggagctggac gatcttctga aggtcacgca
     1201 gaacacgttg aagacctcgg aggaatcgta tcggatcgcc aagttgcagt tcgacaacgg
     1261 cactggctcc gagctcgatc tgcgccagtc gcaaacagtg gtcgagacgg ccagcgcgaa
     1321 tctgcagtcg caggatcgtc tgcgggcgca ggctgaaaac gcgctggtgc tgctggtcgg
     1381 cgaaccgctg cccgccgatt tgccgccggg tctaacgttg aacgaccaga atctcctgac
     1441 ggatattccg gctggtttgc cgtccgatct gctgacgcgt cgtccggaca tcatggaggc
     1501 cgaggagaat ctgctcgcgg ccaacgcgaa tatcggcgcg gcgcgcgcgg cgttcttccc
     1561 gaaggtctcg ctgaccggca gtttcggaac actgagcccg tcgctcggcg gtctgttcaa
     1621 accgggttcg gcggcatgga gcttcgcgcc gtcgatcacg ctgccgattt tcgagggcgg
     1681 tcagaacctg gccaatctcg atctcgccaa catccagaag aaagtccaga ttgccacgta
     1741 cgaaaaggcg atccagacgg ccttccgcga agtggcggac gggctggcgg cacgcggcac
     1801 gtacgatcag caaatccagg cgctggagcg cgataccttt gcggaacaac gccggctgga
     1861 tctgtcgaat ctgcgttata cgaacggtgt cgacagctac ctgtcggtgc tgaccgcgca
     1921 gaccgatctg tatgcggcgc aactgttgtt gactaccgcg cgcatggagc gtctgcagaa
     1981 cctggtcacg ctgtatcagg cgctcggcgg tggctggatc gagcggaacg gcgaacagcc
     2041 gcgtccggcc gacgcgccgg tcgattacgg cgcggccagt gcgccggttg cagcttcggc
     2101 ggcgacagcg ggctgagtct cgcggggcgc aaggcgcgtt tcgtttcctc ggaagctgcg
     2161 ctatgcagcg ggtgggacag gcagattttc acggtttcag aaaggctgct ctgtccaacc
     2221 ggtaacaaaa aacgccacgg catgccgtgg cgtttttatt tggtgcgctg aatgcgcgtg
     2281 acttcagcaa ccctcgcgga cctcaagcac tcaagcacct cgcgcacctc aagcacctca
     2341 agcacctcgc gcacctcaag cacctcaagc acctcaagca cctcaagcac ctcaagcacc
     2401 tcaagcacct caagcacctc aagcacctca ccgacgccag tcgcgtggcc ccggttcccc
     2461 aaattcactg caaatcgatc gcccgcagca tttcgtcgcg tggcggctca ttcccgccgc
     2521 gtccgtcgaa atcatgcccg gccttgcgaa tttcgcactg cgccttcttc tcgctcttca
     2581 caccgttgaa gatcagattc agcaccaccg ccgataccga agccagcagg atcccgctat
     2641 gcaacagcgg cgacagcgcc ggcggcagct tcgagaaaaa ttgtggcgac accaccggca
     2701 ccagacccat gccgatactc accgcgacga tgaacaggtt gtgatggttc ttcacgaagt
     2761 cgaccttggc cagcaccttg ataccgttcg ccgcgaccat gccgaacatc acgatgcccg
     2821 cgccgcccag cacgaaggcc ggcaccgaag ccaccacctg cgccatcttc ggaaacagac
     2881 ccagcagcac caggatcaca ccgcccatcg cgcagacaaa gcggctcttc acccccgtca
     2941 cgccgatcag gccgacgttc tgcgagaacg aggtatgcgg aaacgagttg aagatgccac
     3001 cgatcaacgt gcccagaccg tcgacgcgca ggccgcgcac cagcgtcttc tgatccaccg
     3061 gacgatcgac catgtcgcca accgcgagga acatcccggt cgattcgatg aaggtaacga
     3121 acatcacggt gaccatcgtt gcgatcgcga gcggatcgaa atgcggcagg ccgaagtgga
     3181 acggcatcac gaagccgacc catggcgcgt gcgtgacgcc ttccatgttg acgcggccaa
     3241 gggccagcgc gatcacgaaa cccgccacga tgccgagcag caccgagata ttggcgatga
     3301 agcccttggc gaacttgttg atgagcagaa tcagcatcaa cacgaccagc gacagcccga
     3361 ggtagatcgg gttgccgtaa tcgggattgc ccacaccgcc ggccgcccag ttgatgccca
     3421 cttccatcag cgacaggccg atcaccgaga tcaccacgcc gatcaccacc ggcgggaaaa
     3481 agcgcagtaa cttgccgacc gctggcgcga gaagaatgcc gatcaccccg gccgc
//
LOCUS       DATASH010000476         3388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1261951,
            whole genome shotgun sequence.
ACCESSION   DATASH010000476 DATASH010000000
VERSION     DATASH010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1261951"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VHV99_13180"
     CDS             <1..184
                     /locus_tag="VHV99_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636420.1"
                     /translation="MISLYVADGDLKAQCINAKVRTPGVSGPEASPRCPLKSHSLARA
                     SHTDAVLPSERHFTDR"
     gene            644..1045
                     /locus_tag="VHV99_13185"
     CDS             644..1045
                     /locus_tag="VHV99_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007741984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEX3636421.1"
                     /translation="MADQTNPFGDLTKTLEQFKVPGVDMAALVASRKKDIEAIVEANR
                     SANDAMQAFARKQTEMLTQAMQDIQAATSAGAADPGKHAEVARNAWLKAVEDVKDLAE
                     IARKSQLAATASLTKRANEHAAEIKQMLQRK"
     gene            1132..2862
                     /gene="phaC"
                     /locus_tag="VHV99_13190"
     CDS             1132..2862
                     /gene="phaC"
                     /locus_tag="VHV99_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HEX3636422.1"
                     /translation="MAAPFNMPDEFVQGFFKSGQTLLHAYTGAAGKIGEVPSTPARMP
                     ALALAEAQAHYWQQQMALYMGMVASAMGQKPTPAVEPERGDRRFSAEAWRENPWYSVL
                     KQTYLLNSRLLNDMIEAAGVGEKDKHKLRFYARQFIDSMSPANFAATNPEAMKTAIET
                     QGESMRVGFANLLGDLKRGRISITDENAFEVGKNVAVSKGSVVFENELFQLIQYAPLT
                     GKVAERPLVIVPPCINKFYILDLQPENSFVRYAAEQGLTVFLVSWRNPDAELGHITWD
                     DYVENGAIKAIEVGRAISGADKVNAVGWCVGGTILSSALAVLRARGDESVASVTLLTT
                     MLDFSDPGDLGVFIDEQGVSQREQTIGKGGIYSGGELGFVFQTLRANDLIWPYVVNNY
                     LKGGAPQAFDLLYWNADGTNLPGPMYAWYLRNMYLENNLCKAGRLSMCGTQVELGRID
                     MPSYIFGTQEDHIVPWKGAYRSTRLVGGDTQFVLGASGHIAGVINPASKNKRSYWSDG
                     TLGDDPQAWLESAKSQPGSWWTHWNNWLQAHAGKQVAAPKKPGNAKYKPIEPAPGRYV
                     KKHPPEVMGA"
BASE COUNT          716 a         1015 c         1065 g          592 t
ORIGIN      
        1 catgatcagc ctctatgttg ccgatggcga tctgaaggcg cagtgtatta acgcgaaagt
       61 ccgtacgccc ggcgtgtcgg ggcccgaggc gtccccgcgc tgtcccctca agagccattc
      121 tctcgccagg gcttcgcata cggacgcagt gctgccttcc gagcgccatt tcaccgaccg
      181 gtaacgccca atgccgcgtc tccagccttc tccgtagttc gcgatctggt ttgtttatac
      241 tgatcggacc gcaaccgtac agacttaccg caaagcaggt gagaccctac ttcttcctcc
      301 tgcgcttatc gacgattttt tgcggcgcct ccacgagagc aggcattggc gaacggctgc
      361 ttaccagtgt tgtccacggg gcattgcagg ccctgttgca taacgaggcg aaaagacacg
      421 cagtgcaggg caacatacgg atggctgatg ttacgcatac tccgtggtag tgactgacgc
      481 ggaagaagcg gacggcgagt cgacgaccgt atgcacatta caaaaaagat ggtaatcgat
      541 gacgatgcgc tcgtgatccc gacgtcaggg cgtgcccgcc cggagtgggt gtaggtagct
      601 aactccatac gtagttagcc gaacaatcca ggagaagaca gtcatggctg atcaaacgaa
      661 tccctttggc gatctgacga agacgctcga acagttcaag gtgccgggcg tcgatatggc
      721 cgcgttggtc gcgtcgcgca agaaggacat tgaggcgatc gtcgaagcga acaggtccgc
      781 taacgacgcg atgcaggcgt ttgcccgcaa gcagaccgag atgctgaccc aggcgatgca
      841 ggacattcag gcagccacca gtgcgggtgc ggcggacccg gggaaacacg ctgaggtggc
      901 gcgcaatgcg tggctgaagg cagtcgaaga tgtaaaggac ctggcagaaa tcgcgcgcaa
      961 gtcgcagctg gcggcaacgg cgagcctcac caaacgcgcg aatgagcacg cggcagagat
     1021 caagcagatg ctgcagcgca aatagctagc gcgccttggc ccgtcgcgcg actggtgcgt
     1081 tgtcatcgcg ggacgggcag caacgtttaa tggtcactct caggaggcag catggccgca
     1141 ccgttcaaca tgccggacga gttcgtccaa ggcttcttta aatcggggca gacgctgctg
     1201 cacgcctaca ccggcgcagc tggcaagatc ggcgaagtgc cttcgacccc cgccaggatg
     1261 ccggcgctcg cactggcaga agcacaggcg cattactggc agcagcagat ggcgctgtac
     1321 atgggcatgg tggcgagcgc aatggggcaa aagcccacgc ccgccgtcga acctgagcgc
     1381 ggcgaccgcc gtttcagtgc cgaggcctgg cgcgaaaatc cgtggtacag cgtgctcaag
     1441 cagacttacc tgctcaactc gcgtctgctc aacgacatga tcgaggctgc gggcgtcggc
     1501 gaaaaggaca agcacaagct gcgtttctac gcgcgccagt tcatcgattc gatgagcccc
     1561 gccaacttcg ccgcgaccaa tcccgaagcg atgaagaccg ccatcgagac gcagggcgaa
     1621 agcatgcgcg tgggctttgc gaacctgctc ggagacctga agcgcggacg catctccata
     1681 actgatgaaa acgcgttcga agtggggaag aacgtcgcag tatcgaaggg atcggtggtg
     1741 ttcgagaatg aactgttcca actgatccag tacgcgccgc tcaccgggaa ggtcgccgag
     1801 cggccgctcg tgattgtgcc gccctgcatc aacaagttct acatcctcga tctgcagccc
     1861 gagaactcgt tcgtgcgcta cgcggccgag caggggctga ccgtgttcct cgtgtcgtgg
     1921 cgcaaccccg acgcagaact cggccacatc acctgggacg attacgtcga aaacggcgcg
     1981 atcaaggcga tcgaagtcgg ccgcgcgatc agcggcgccg acaaggtcaa tgcagtcggc
     2041 tggtgtgtcg gcgggacgat cctgtcgtcg gcgctcgcgg tgctgcgcgc gcgcggcgac
     2101 gagtcggtcg cgagcgtgac gctcctcacg acgatgctcg acttcagcga tcccggcgac
     2161 ctcggcgtgt tcatcgacga gcagggcgtg tcgcagcgcg agcagacgat cggcaagggc
     2221 ggcatctatt ccggcggtga actcggcttc gtgttccaga cgctgcgcgc gaacgacctg
     2281 atctggccgt atgtcgtgaa caactacctt aagggcggcg cgccgcaggc gttcgacctg
     2341 ctctactgga acgccgacgg caccaatctg cccggcccga tgtacgcctg gtacttgcgc
     2401 aacatgtatc tcgaaaacaa tctgtgcaag gcgggccgcc tctcgatgtg cggcacgcag
     2461 gtggagctcg gccgcatcga catgccaagc tatatcttcg gcacgcagga agatcacatc
     2521 gtgccgtgga agggcgcgta tcgttcgaca cgactcgtcg gaggtgacac gcagttcgtg
     2581 ctcggcgcga gcgggcatat cgcgggcgtg attaatccgg cgtcaaagaa caagcgcagc
     2641 tactggagcg acggcacgct cggtgacgat ccgcaggcgt ggctggagtc ggcgaaatcg
     2701 cagcccggaa gctggtggac gcactggaac aactggctgc aggcgcatgc gggcaagcag
     2761 gtcgcagcgc cgaagaagcc gggcaatgcg aagtacaagc cgatcgagcc ggcgcctggg
     2821 cgttatgtga aaaaacatcc cccggaagtc atgggcgcgt gagcggcgct gggcgcttca
     2881 aacccgggcg acggacgcct agaagtgcaa ctcatcgcgc actgctgccg acggccagtg
     2941 tgcaagcccg tgatcgcggc catcacagcc tccagcgccg gggtaccggc cgctggtcag
     3001 cttacgcgcc gcgcaggcgg acatttgaac ttggctataa gcggacatta ctacttgggt
     3061 cttacacaca caatgttgat aatttatgtt atgtcaaata aaagctcggg atggcactaa
     3121 gctacttcga cccctgctca gcctatgcgc gaaagttcac gaggctgaac cgcgcgcgtg
     3181 ggcgggccct gcacgcggac tttattgccg cgctgaacgc acgcatcgag cacgacggcc
     3241 tgccgctcga cgaacacagg atgttctgat gacgcgcttc gatctgtacg acaacccttc
     3301 acctaggtcc cgagcgaagt cgccgtacct cctcgatgtg cagagcgatc atctgggcag
     3361 tctgatctcg cgcatcgtta ttccgctc
//
LOCUS       DATASH010000477         6766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6403795,
            whole genome shotgun sequence.
ACCESSION   DATASH010000477 DATASH010000000
VERSION     DATASH010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6766
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6403795"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..1957
                     /gene="dxs"
                     /locus_tag="VHV99_13195"
     CDS             50..1957
                     /gene="dxs"
                     /locus_tag="VHV99_13195"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012338492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEX3636423.1"
                     /translation="MYDLLKTIDDPAALRRLDRRQLQPLADELRAFVLDSVSQTGGHL
                     SSNLGTVELTIALHYVFDTPRDRIVWDVGHQTYPHKILTGRRDQMHTLRQLGGISGFP
                     KRDESEYDTFGTAHSSTSISAALGMAVASKLQGENRMGIAVIGDGAMTAGMAFEAMNN
                     AGVEEDVPLLVILNDNDMSISPPVGALNRHLARLMSGRFYAAARAGVERVLRVAPPML
                     DLARKLEEHAKGMIVPATLFEEFGFNYIGPIDGHDLDSLIPTLQNIKELRGPQFLHVV
                     TKKGQGYKLAEADPVLYHGPGKFNPAEGIKPAATPSKKTYTQVFGEWLCDAAELDARV
                     IGITPAMREGSGMVEFEKRFPDRYFDVGIAEQHAVTFAGGLAADGMKPVVAIYSTFLQ
                     RAYDQLIHDVALQNLPVVFAIDRAGLVGADGATHAGAYDLAFLRCIPNMTVMAASDEN
                     ECRQMLYTALQQPNPTAVRYPRGAGTGVATVKQMTALPLGKGEIRRETSQPAGRRIAI
                     LAFGTMVAPSLAAAEQLDATVANMRFVKPLDAELVRQLAETHDAIVTVEEGCVMGGAG
                     SACVEALLESGVIRPVLQLGLPDRFIDHGDPAKLLAACGLDAAGIAKSIRERFLSGGT
                     VAGQSSVKRVA"
     gene            2323..3129
                     /gene="folE2"
                     /locus_tag="VHV99_13200"
     CDS             2323..3129
                     /gene="folE2"
                     /locus_tag="VHV99_13200"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HEX3636424.1"
                     /translation="MNQMNPAFVMPDVQSTLDTRQIPIQRVGVKAVRHPLTVRTQGGE
                     VQPTVGTWNLDVHLPADQKGTHMSRFVALLEENKAPLESATFRTMLAAMLEKLEAQAG
                     RIEVSFPYFVNKTAPVSGVQSLLDYEVTLTGEARHGATRLFLKVLVPVTSLCPCSKKI
                     SQYGAHNQRSHVTINAELESDVAVEELVRIAEEEASCELWGLLKRPDEKFVTERAYEN
                     PKFVEDLVRDVAQRLNADERIVAYVLEAENFESIHNHSAYAVIERDKRAA"
     gene            complement(3204..4232)
                     /gene="tsaD"
                     /locus_tag="VHV99_13205"
     CDS             complement(3204..4232)
                     /gene="tsaD"
                     /locus_tag="VHV99_13205"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX3636425.1"
                     /translation="MLVLGIESSCDETGLALYDTERGLLAHALHSQIAMHREYGGVVP
                     ELASRDHIRRALPLLEEVMERAGTVRGDIDAIAYTQGPGLAGALLVGASVANSLAMAW
                     DKPTIGIHHLEGHLLSPLLVDEPPPFPFVALLVSGGHTQLMRVTDVGVYETLGETLDD
                     AAGEAFDKTAKLLGLGYPGGPEVSRMAEFGTPGAVVLPRPMLHSGDLDFSFSGLKTAV
                     LTHARKLGGANLCEQAKADLARGFVDAAVDVLAAKSLAALKRTQLNRLVVAGGVGANR
                     QLREALSAAAKKRNFFVHYPDLSLCTDNGAMIALAGALRLQRWPDQSAKDYAFTVKPR
                     WDLTSLAR"
     gene            4355..5572
                     /locus_tag="VHV99_13210"
     CDS             4355..5572
                     /locus_tag="VHV99_13210"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX3636426.1"
                     /translation="MESFDIAVIGAGAAGMMCAAVAGQLGRRVVLIDHSQRLAEKIRI
                     SGGGRCNFTNLYAGPANYLSANPHFCRSALARYTPRDFMALLKRHHVTWHEKHKGQLF
                     CDQSSDAVINVLKSECDAGRIAWRTPLAVEQVRHDVEGRFTLDTHAGPITARALVVAT
                     GGLSIPKIGATDFAYRLARQFGHKLIDTRPALVPLTFTPADWEPFSALSGVSLEVQLA
                     TGNKKTGAEFNEDLLLTHRGLSGPGVLQISSYWHPGEPIHINLLPEQDATTALLEAKT
                     GTRRQIANLLSEWVPQRLAHVWLETHQISAEARVADLPDKTLRRVGEALSRWTLTPNG
                     TEGYRKAEVTRGGIDTRDLSSATMMSARTPGLYFIGEAVDVTGWLGGYNFQWAWASGV
                     AAGQAAAEYVLGA"
     gene            5641..5853
                     /gene="rpsU"
                     /locus_tag="VHV99_13215"
     CDS             5641..5853
                     /gene="rpsU"
                     /locus_tag="VHV99_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEX3636427.1"
                     /translation="MTIIRVKDNEPFEVAMRRFKRTMEKNGLLTELRDREFYEKPTAE
                     RKRKKAAAVKRHFKRLRSQMLPRKFY"
     gene            6114..6560
                     /locus_tag="VHV99_13220"
     CDS             6114..6560
                     /locus_tag="VHV99_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014900012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEX3636428.1"
                     /translation="MSLKDQINDDMKAAMRARETERLGTIRLLLAAVKQREVDERVTL
                     DDAAITAVIDKMIKQRKDSISQFEGAGRTDLADKEKAELAILSAYMPAQMSEAEIVAE
                     VQDAVVQTGAAGPQDMGKVMGVLKPKLAGRADMTAVSAQVKAALAK"
     gene            6639..>6766
                     /locus_tag="VHV99_13225"
     CDS             6639..>6766
                     /locus_tag="VHV99_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HEX3636429.1"
                     /translation="MIPPSFLQDLLNRVDIVDVVGRYVQLKKGGANFMGLCPFHNE"
BASE COUNT         1281 a         2088 c         2156 g         1241 t
ORIGIN      
        1 gtgcatgtat gaagtgcggg cgcgtaagtt ttcctacaat ggaacgacga tgtacgactt
       61 gctgaaaacc atcgacgacc cggcagcctt gcgccgcctc gatcgccgcc aattgcaacc
      121 gcttgccgac gagttgcgtg cctttgtgct cgacagcgta tcgcagacgg gcggccatct
      181 ttcgtccaac ctcggcacgg tcgagttgac cattgctttg cactatgtgt ttgacacgcc
      241 acgtgaccgg atcgtgtggg acgtcggcca tcaaacctat ccgcacaaga tactgacggg
      301 ccgccgcgac cagatgcaca cgttgcgtca gctcggcggc atctcgggct ttccgaagcg
      361 cgacgagtcg gaatacgaca ccttcggcac cgcgcactcc agcacctcga tctcggccgc
      421 gctcggcatg gcggtcgcca gcaagctgca gggcgagaac cgtatgggca tcgcggtgat
      481 cggcgacggc gcgatgacgg ccggcatggc tttcgaggcg atgaacaacg cgggcgtcga
      541 agaggacgtg ccgctgctgg tcatcctgaa cgacaacgac atgtcgattt cgccgccggt
      601 cggcgcgctg aatcgtcacc tggcgcgcct gatgtcgggc cgcttctacg ctgccgcgcg
      661 tgcgggcgtc gagcgcgtgt tgcgcgttgc accgcctatg ctcgatctgg cgcgcaagct
      721 cgaggagcac gccaagggca tgatcgtgcc ggccacgctg ttcgaagagt tcggtttcaa
      781 ctacatcggc ccgatcgacg gtcacgatct ggattcgctg atcccgacac tgcagaacat
      841 caaggaactg cgtggtccgc aattcctgca cgtcgtgacg aagaagggcc agggctacaa
      901 gctggccgaa gccgacccgg tgctgtacca cggcccgggc aagttcaacc cggctgaagg
      961 catcaagccc gccgccacgc cgtcgaagaa aacctacacg caagtgttcg gcgaatggtt
     1021 gtgcgacgcg gcagaactgg atgcgcgcgt gatcggtatc acgccggcca tgcgtgaagg
     1081 ttcgggcatg gtcgagttcg agaagcgctt cccggatcgc tacttcgacg tcggcattgc
     1141 cgagcagcac gcggtgacgt ttgccggtgg tctggctgcg gacggcatga agccggtggt
     1201 tgcgatttat tcgaccttcc tgcaacgcgc ttatgaccag ctgatccacg acgtcgcgct
     1261 gcaaaacctg ccggtcgtgt tcgcaatcga ccgcgcgggt ctggtcggcg cggacggcgc
     1321 cacgcacgcg ggcgcctatg atctggcgtt cctgcgctgc atcccgaaca tgacggtgat
     1381 ggcggcatcg gacgaaaacg aatgccgtca gatgctttac accgcattgc agcagccgaa
     1441 cccgacggcg gtgcgctatc cgcgcggcgc cggcacgggt gtcgcaactg tcaagcagat
     1501 gacggcgctg ccgctcggca agggcgagat tcgtcgcgag acgtcgcaac cggccggcag
     1561 gcggattgcg atcctggcgt tcggcacgat ggtcgcaccg tcgctggcgg ctgctgagca
     1621 actggacgcc acggtggcga acatgcgctt cgtgaagccg ttggacgccg aactggtccg
     1681 ccaactggcc gaaacgcacg acgccatcgt cacagtcgaa gaaggttgcg tgatgggcgg
     1741 tgcgggttcg gcttgcgtcg aagccctgct tgagagtggg gttatacgac ccgtactaca
     1801 attgggcctt ccggatcgct tcatcgatca tggggatccg gccaagctgc tcgccgcctg
     1861 cggtctggac gcagcgggca tcgccaagtc gattcgtgag cgctttcttt ccggcggcac
     1921 ggtcgccggt caatcctcgg tgaagcgcgt cgcgtagttg agtcgcttgt ttgtggcgct
     1981 tgaatagctt cgcttacttg cgtcacttga ctccgcgccg attgctgagt cgtccagctg
     2041 ctctgccgtg cgcttcgccg cggtgatagc ggccatagca atctccaact tgaatcgatg
     2101 gaccctcggg tccatcatcc agcgccggca ggggccggcg tttgccgttg cgcagtctga
     2161 tccgcgcggt cgccgagcac ccctaaccct gttgcttcgc gacggccccc cacggggcaa
     2221 tcccccgggg gatggcctgc ggggcgcgag atttccttcg gaaggacttc ccgcgaagcg
     2281 aaaacctggg gccgcccggc ggtttcctga aaggacaaga atatgaacca gatgaatccc
     2341 gcctttgtga tgcccgacgt gcagagcacg ctcgatacgc gtcagattcc gatccaacgc
     2401 gtcggcgtca aggccgtgcg ccatccgttg acagtgcgca cgcaaggcgg cgaagtgcag
     2461 cccacggtcg gcacatggaa tctcgatgtg catctgcccg ccgatcagaa gggcacgcat
     2521 atgtcgcgct tcgtcgcgtt gcttgaagag aacaaggcgc cgctcgagtc ggccaccttc
     2581 cgcaccatgc tcgccgcgat gctcgaaaag ctcgaggctc aggcgggtcg catcgaggta
     2641 tcgttcccgt acttcgtgaa caagacggcg ccggtgtcgg gcgtgcaaag tctgctcgac
     2701 tatgaagtga cgttgacggg cgaggctcgc catggcgcga cgcgtctgtt tctgaaggta
     2761 ctggtgccgg tcacgagcct gtgcccgtgc tcgaagaaga tttcgcagta cggcgcgcac
     2821 aaccagcgtt cgcacgtcac gatcaacgcc gaactggaga gcgacgtggc agtggaagaa
     2881 ctggttcgta tcgctgaaga agaagcctcg tgcgaactct ggggcttgct caagcgtccg
     2941 gacgagaagt tcgtcaccga acgtgcttac gagaatccaa agttcgtcga agatctggtg
     3001 cgcgatgtcg cacagcgtct gaacgcggac gagcgcatcg tcgcttatgt gctcgaagct
     3061 gagaacttcg aatcgattca caatcacagc gcgtacgcag tgatcgagcg ggataagcgg
     3121 gcggcctgag tggcgcgttt cgttgcttga atgagaaagc cgctcttccg agcggctttt
     3181 ttacgccctc acgtcgctga aattcagcgt gcgagggaag tcagatccca gcgcggcttc
     3241 accgtgaacg cataatcttt ggccgattga tcgggccagc gttgcagtcg caatgcgccg
     3301 gccagcgcaa tcatcgcgcc gttgtccgta cacagcgaca ggtccgggta gtgcacgaag
     3361 aaattgcgtt tcttagctgc agctgaaagc gcctcgcgca gttgccggtt cgcgccgaca
     3421 ccgcccgcga cgaccagccg gttgagctgg gtccgtttga gcgcggccag cgattttgcc
     3481 gccagcacgt ccacggccgc atccacgaac ccgcgtgcca gatcggcttt cgcctgctcg
     3541 cagagattcg cgccaccgag tttcctggca tgcgtgagga ccgcggtctt caggccgctg
     3601 aagctgaaat cgaggtcgcc ggaatgcagc atcggacgcg gcagaaccac tgcgccaggc
     3661 gtgccgaatt cggccatccg cgatacttcc gggccgcccg gatagccgag gcccagcagc
     3721 ttggcggttt tgtcgaaggc ttcgccggcg gcgtcgtcca gcgtttcgcc gagcgtctcg
     3781 tagacgccca cgtccgtcac gcgcatcaac tgcgtatggc cgcccgacac cagtagcgcc
     3841 acgaacggaa acggcggcgg ctcgtccacc agcagcggcg acagcaagtg cccttcgaga
     3901 tggtgaatgc cgatggtcgg cttgtcccac gccatggcca gcgaattggc gacgctcgcg
     3961 ccgaccagca gcgcgcccgc gaggccaggg ccttgcgtat aggcgattgc gtcgatatct
     4021 ccgcgcaccg tgccggcgcg ctccatcacc tcttcgagca gcggcagcgc ccggcggatg
     4081 tgatcgcgcg acgccagttc cggcaccacg ccgccatact cccgatgcat cgcaatttgc
     4141 gaatgcagcg cgtgcgcgag caggccgcgc tctgtgtcgt agagcgcgag accggtttcg
     4201 tcgcaggagc tttcgatgcc gagaacgagc atgatgggtc gcttgaaaag gcgcgcgagt
     4261 gaagacactg gggcgcccaa aaagtgaaaa ggcaagcctg aaagtatagc agcgcgggca
     4321 gggggccgcg ggcgcacggg tacaatgcgg ccccatggaa tccttcgata tcgcagtgat
     4381 cggcgcaggc gcggccggca tgatgtgcgc ggcagtggct gggcaactcg gccgtcgcgt
     4441 ggtgctgatc gaccactcgc agcgcctcgc ggagaaaatc cgcatttccg gtggcggccg
     4501 ctgcaacttc acgaatctgt atgccgggcc ggccaactat ctgtcggcca acccccactt
     4561 ttgccgctcg gcgctggcac gctatacgcc gcgtgacttc atggcgctgc tcaagcgcca
     4621 tcacgtgacg tggcacgaga agcacaaggg gcagctgttt tgcgaccaat cgagcgacgc
     4681 ggtcatcaat gtgctgaaga gcgagtgtga cgcgggccgc attgcgtggc gcacgccgct
     4741 tgcggtcgag caggtgcggc atgacgtcga aggacgattt acgctggaca cccacgcggg
     4801 cccgatcacg gcccgcgcgc tcgtggtggc gaccggcggc ctgtcgattc ccaagattgg
     4861 cgctaccgat ttcgcctacc gcctcgccag gcaattcggc cacaagctga tcgacacgcg
     4921 ccccgcgctc gttccgctga ccttcacccc ggctgactgg gagccgtttt cggcgctttc
     4981 cggcgtgtcg ctcgaagtgc aactggcgac cggcaataaa aagaccggtg ccgaattcaa
     5041 cgaagacctg ctgctcacgc atcgcggcct gtcgggcccc ggcgtgctgc agatttcgag
     5101 ctactggcat ccgggcgagc cgattcacat caatttgctg cccgagcagg acgccacgac
     5161 ggccttgctg gaggccaaaa ccggcaccag gcgccagatc gccaatctgc tttcggaatg
     5221 ggtgccgcaa cggctcgctc atgtctggct ggaaacccac cagatttccg ccgaagcccg
     5281 cgtcgccgat ctgcccgaca aaacgctgcg ccgcgtcgga gaggctttgt cgcgctggac
     5341 gctcaccccg aacggtaccg aaggctatcg caaggccgaa gtcacgcgcg gtggtatcga
     5401 cacgcgcgac ctctcgtccg cgaccatgat gagcgcccgc acgccgggct tgtacttcat
     5461 cggcgaagcg gtcgacgtga ccggctggct cggcggctac aatttccaat gggcgtgggc
     5521 ttcgggcgtg gcggccggcc aggcggccgc ggagtacgtt ttgggggctt gacggcgcgt
     5581 tagctttgtg agaaagctct gctatactcc tgaaccttta caaagttccg ttattgaaaa
     5641 atgacgatca tccgcgtaaa agacaacgag ccgtttgaag tcgccatgcg ccgcttcaag
     5701 cgcacgatgg agaaaaacgg gttgctgacg gaactccgcg accgagagtt ttacgaaaag
     5761 cctacggccg agcgcaagcg caagaaggcg gctgcggtga agcgtcattt caagcgtctg
     5821 cgtagccaga tgctgccaag gaagttctac tgattctggc gttgccgcgg tttccgtcga
     5881 agggagcggc gacatccaac cggtgagggc acagcaggtg ccgtcacgga aaatacggcc
     5941 cttcgggcca gccggcaggg tcgcatccac aacgcaaaat tgcgccaacc cgcttgagga
     6001 agcagtccca agcgggcgtt tgtgttggat gccgcgtgcg aaccttgcgt attggttcgg
     6061 catgtcggtt cattagcggc cggtttttaa atttcaacgt attcaggtga gtgatgagtc
     6121 tcaaggacca gatcaacgac gatatgaaag cagccatgcg cgcgcgtgag accgagcgtc
     6181 tcggcacgat ccgcttgctg cttgccgcgg tcaagcaacg cgaagtcgac gaacgcgtca
     6241 cccttgacga cgcggcgatc accgccgtca tcgacaagat gatcaagcag cgcaaagact
     6301 cgatcagcca gtttgaaggc gccggccgca cggatctcgc cgacaaggaa aaggccgaac
     6361 tggccattct gtccgcctat atgccggcgc agatgtccga agccgagatc gttgccgaag
     6421 ttcaggacgc ggtcgtgcaa accggcgctg ccggcccgca ggacatgggc aaggtgatgg
     6481 gcgtgctcaa gccgaagctg gctggccgtg ccgacatgac cgccgtttcg gcgcaagtca
     6541 aagccgccct cgcgaagtaa ttcctgtaat tccggtctgc gctcgctgtt gtccgatagc
     6601 gagcgcagtc ttgagctttt taggtagcgt cattcactgt gattccaccg tcattcctgc
     6661 aggacctgct caaccgcgtc gatatcgtcg acgtggtcgg ccggtatgtg cagttgaaaa
     6721 agggcggcgc gaacttcatg gggctgtgcc cgtttcacaa cgagaa
//
LOCUS       DATASH010000478         1531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2842959,
            whole genome shotgun sequence.
ACCESSION   DATASH010000478 DATASH010000000
VERSION     DATASH010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2842959"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1521)
                     /locus_tag="VHV99_13230"
     CDS             complement(<1..1521)
                     /locus_tag="VHV99_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019254711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HEX3636430.1"
                     /translation="MTETVALKIVQRIAAELSVQPRQVAAAVQLLDEGATVPFIARYR
                     KEVTDNLDDTQLRNLEERLLYLRELEDRRAAIISSIDEQGKLTDELRTAIEGADSKQV
                     LEDLYLPYKPKRRTRAQIAREAGLQPLADALLANPLLDPQTEAAAYVDAEKGVADVKA
                     ALDGARDILSEQFGETADLLGKLRDYLFNQGVVSSKVVEGKENAEEEKFRDYYDYAET
                     IRTVPSHRALALFRGRNAGVLMVKLGLGEELDAQVPHPGEALIARHFGIANQNRPADK
                     WLSDVCRWCWRVKVQPHIENELLTNLRDEAEHEAIRVFARNLKDLLLAAPAGPKAVVG
                     LDPGMRTGVKVAVVDRTGKVLATDVIYPHEPRRDWDGSIAKLARICAQTQAELISIGN
                     GTASRETDKLASELIARHPEFKLQKIVVSEAGASVYSASELAAKEFPDMDVSLRGAVS
                     IARRLQDPLAELVKIEPKAIGVGQYQHDVNQRDLAGSLDAVVEDCVNAVGVDANTAS"
BASE COUNT          234 a          487 c          493 g          317 t
ORIGIN      
        1 cgatgccgta ttcgcatcca cgccgaccgc gttcacacaa tcctcgacca ccgcgtcgag
       61 cgagccagcc aggtcgcgct gattcacgtc gtgctggtat tggccgacgc cgatcgcctt
      121 gggttcgatc ttcacgagtt ccgcgagcgg atcctgcaga cgacgcgcaa tcgacacggc
      181 gccgcgcagc gacacgtcca tgtccgggaa ttcctttgcc gcgagttcgg aggccgagta
      241 caccgatgcg ccggcttccg agacgacgat cttctgcagc ttgaactccg gatgacgtgc
      301 gatcagttcg ctcgccagct tgtccgtttc acgcgacgcc gtgccgttgc cgatactgat
      361 caactcggcc tgcgtttgcg cgcagatgcg ggccagtttc gcgatcgagc cgtcccagtc
      421 gcggcgcggc tcgtgcggat aaatcacgtc ggtggcgagc accttgccag tccgatcgac
      481 caccgcgact ttcaccccgg tgcgcatacc cggatcgagg ccgaccacgg ccttcgggcc
      541 cgcgggcgcg gccagcagca ggtccttcag attgcgtgcg aacacgcgaa tggcttcgtg
      601 ctcagcttcg tcgcgcagat tggtcagcag ttcgttttcg atgtgcggct gtaccttcac
      661 gcgccagcac cagcggcaca catcggagag ccacttgtcg gccgggcggt tctgattggc
      721 aatgccgaag tggcgtgcga tcagtgcttc gcccggatgc ggcacctgtg cgtcgagttc
      781 ttcgccaagc cccagcttga ccatcagcac gcccgcattg cggccgcgga acagggccag
      841 tgcgcgatgc gacggcacgg tccggatggt ttcggcatag tcgtagtaat cgcggaattt
      901 ctcttcttcc gcattttcct tgccctcgac cactttcgac gacaccacgc cctggttgaa
      961 caggtaatcg cgcagcttgc cgagcagatc cgcggtttcg ccgaactgtt cggagaggat
     1021 gtcgcgcgcg ccgtcgagcg cggccttgac gtcggcgacg cctttctcgg cgtccacgta
     1081 ggcggcggct tcggtttgcg ggtcgagcag gggattggcc agcagtgcgt ctgcaagcgg
     1141 ctggaggccg gcttcgcggg cgatctgcgc gcgtgtgcgg cgcttgggct tatacgggag
     1201 atacaggtct tccagcacct gcttgctgtc ggcgccttcg atcgcggtgc gcagctcgtc
     1261 ggtgagcttg ccttgttcgt cgatgctcga aatgatggcg gcgcgccggt cttccagctc
     1321 gcgcaggtac agcaggcgct cctcgagatt acgcagttgc gtgtcgtcga gattgtcggt
     1381 cacttccttc cggtagcggg cgatgaacgg aacagtcgct ccttcgtcga gaagttgcac
     1441 cgcggccgcg acctggcgcg gctggacgga cagttcggcg gcgatgcgct gtacgatctt
     1501 gagtgctacg gtttccgtca taaggtcttg g
//
LOCUS       DATASH010000479         1850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5614983,
            whole genome shotgun sequence.
ACCESSION   DATASH010000479 DATASH010000000
VERSION     DATASH010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5614983"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="VHV99_13235"
     CDS             complement(<1..365)
                     /locus_tag="VHV99_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEX3636431.1"
                     /translation="MRDEFDEPQPALETPSVAEVTAAEAPAPLLAQVPAGIRLLNRDF
                     LTDVANIPDGSIDLILCDPPYGLGKDYGNDSDMRSGEDFLAWTRGWLELAIPKLKPSG
                     SLYIFCTWQYAPEIFSFLKT"
     gene            complement(377..1570)
                     /gene="trpB"
                     /locus_tag="VHV99_13240"
     CDS             complement(377..1570)
                     /gene="trpB"
                     /locus_tag="VHV99_13240"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX3636432.1"
                     /translation="MYNLPDERGHFGQYGGVFVAETLVHALDELRVAYEKYQKDPEFV
                     AEYERELKYFVGRPSPIYHAQRWSEMLGGAQVFFKREDLNHTGAHKINNVIGQALLAK
                     RMGKPRVIAETGAGQHGVATATIAARFGMECVVYMGEEDVRRQAANVYRMKLLGATVV
                     PVSSGSRTLKDALNEAMRDWVTNVENTFYIIGTVAGPHPYPMMVRDFQRVIGDECKVQ
                     MPELVGRQPDAVIACVGGGSNAMGIFYPYIDDTSVQLIGVEAAGDGIESGRHAASLIG
                     GSPGVLHGNRTYLLQDDNGQIIETHSVSAGLDYPGVGPEHAWLKDSNRAQYVGITDEE
                     ALKAFHDCCRIEGIIPALESSHALAYAAKLAPTLPKDKVLLVNLSGRGDKDMHTVAER
                     SGIQF"
     gene            complement(1631..>1850)
                     /locus_tag="VHV99_13245"
     CDS             complement(1631..>1850)
                     /locus_tag="VHV99_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-(5'-phosphoribosyl)anthranilate isomerase"
                     /protein_id="HEX3636433.1"
                     /translation="KVFDWSLIPAELARRAVLSGGLNAQNVSDAIHRVRPYAVDVSSG
                     IEVPGAKGVKDHARMAAFVRAVRAADAE"
BASE COUNT          360 a          582 c          542 g          366 t
ORIGIN      
        1 gtcttcagga agctgaagat ttccggggcg tactgccacg tgcagaagat atacagcgaa
       61 cccgacggct tgagcttcgg aatagccagt tcgagccagc cacgcgtcca ggcgaggaag
      121 tcttcgccgg agcgcatgtc ggagtcgttg ccataatcct tgccgagccc ataaggcgga
      181 tcgcaaagga tcaggtcgat cgacccgtcg gggatgttcg ccacatcggt caaaaaatcg
      241 cggttcaaca gccggatgcc ggcgggcacc tgggctaaca aaggtgcagg cgcctcggcc
      301 gccgttactt cggcgaccga aggggtttca agcgccggtt gcggctcgtc gaactcgtca
      361 cgcatcgtcg cggcgctcag aactgaatgc ccgatcgctc agcgaccgtg tgcatgtcct
      421 tgtcgccgcg gccagacagg ttgaccagaa ggaccttgtc cttcggcagt gtcggcgcaa
      481 gcttcgcggc gtaggccagc gcatggctgg attcgagcgc cggaataatg ccttcgatgc
      541 gacagcaatc gtggaacgcc ttgagcgcct cttcgtcggt aatgccgaca tattgcgcgc
      601 gattgctgtc ttttagccac gcatgctccg gaccgacgcc cgggtagtcc agaccggccg
      661 agaccgaatg cgtctcgatg atctgaccgt tgtcgtcttg caggagatag gtgcggttgc
      721 cgtgcagcac gcccggactg ccgccgatca gcgaggccgc gtgacgaccg gattcgattc
      781 cgtcgcctgc tgcctcgacc ccgatcaatt gcaccgaagt atcgtcaatg tacgggtaaa
      841 aaatgcccat tgcatttgaa ccaccgccaa cgcatgcaat gacggcatcc ggctgacggc
      901 cgaccagttc gggcatctgc accttgcact cgtcaccgat cacccgctgg aagtcgcgca
      961 ccatcatcgg atacggatgg ggtcccgcga cagtgccgat gatgtagaag gtattttcga
     1021 cgttggtgac ccaatcgcgc atcgcttcat tgagggcgtc tttcagcgtg cgcgagccgg
     1081 acgacaccgg cacgacggtc gccccgagca gcttcatccg gtacacgttg gcagcctggc
     1141 ggcgcacatc ttcctcgccc atgtagacca cgcattccat gccgaagcgg gccgcaatcg
     1201 ttgcggtcgc cacgccgtgc tgacccgcac cggtttccgc gatcacgcgc ggcttaccca
     1261 tgcgcttggc gagtagcgcc tggccgatca cgttattgat cttgtgggcg ccggtgtgat
     1321 tcagatcttc acgcttgaag aaaacctgcg cgccgccgag catttcgctc cagcgctgcg
     1381 catgataaat gggggacgga cgacccacga aatacttcag ttcgcgctcg tattcggcga
     1441 cgaattccgg gtctttctga tatttctcgt acgcgacacg caactcgtcg agcgcatgaa
     1501 ccagcgtttc agcgacgaac acgccgccat attggccgaa atggcctctt tcgtcaggca
     1561 agttatacat ggtgatcact cttctcagtg tggcgcacgg ttttcgtgtg gagaaaccgc
     1621 gccgcctgaa tcattcggcg tctgccgcgc gcactgcgcg tacgaacgcc gccatccggg
     1681 cgtgatcctt cacgcccttg gcgcccggca cttcgatgcc gctcgagaca tcgaccgcgt
     1741 acgggcgcac gcgatggatc gcatcactga cgttttgcgc gttcaaccca ccactcaaaa
     1801 cggcccgacg cgcgagctct gctggaataa gtgaccaatc gaaaaccttc
//
LOCUS       DATASH010000480         4660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4229900,
            whole genome shotgun sequence.
ACCESSION   DATASH010000480 DATASH010000000
VERSION     DATASH010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4660
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4229900"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..315
                     /locus_tag="VHV99_13250"
     CDS             193..315
                     /locus_tag="VHV99_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636434.1"
                     /translation="MSFRVASQKLVRGALPSCRGSRLHGVTLEPGVLICGDGVG"
     gene            complement(459..694)
                     /locus_tag="VHV99_13255"
                     /pseudo
     CDS             complement(459..694)
                     /locus_tag="VHV99_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489436.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
     gene            complement(753..1907)
                     /locus_tag="VHV99_13260"
     CDS             complement(753..1907)
                     /locus_tag="VHV99_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018438112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HEX3636435.1"
                     /translation="MQIEPFQIAVPDADIDDLRRRIRATRWAPATPSPPWQQGADSAW
                     LRELAQYWADRFDWREAERRLNLQPQFLSEVEGQRVHFVHRRGVGPASYPLVVTHGWP
                     GSFFEFHALLERLCDPSAFGCDPADAFDVVVPSLPGFAFSQAPVEPGTSAFQVADLWA
                     SLMRGLGYEHFGAQGGDLGAGVSVALAARHHDVVDGIHLNFLPSSYEPANDATQTPLT
                     PAEESYLREKNEWAALEGGYAHMHATKPQTVAASLNDSPVGLAAWIGEKFRAWSDCDG
                     DIERVFSKDELLTNISLYWHTQSAGTAMQMYWENRLQPMRFTDGQRIVPPVGFARFPK
                     EIHHPPRTWLERTFNVVQWTDMPSGGHFAAMEKPDLLAAEIRKFFRKLRR"
     gene            2256..3035
                     /gene="surE"
                     /locus_tag="VHV99_13265"
     CDS             2256..3035
                     /gene="surE"
                     /locus_tag="VHV99_13265"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEX3636436.1"
                     /translation="MSAYESKVPRVLLTNDDGIDAPGLAVLEAVAAELAHEVWVVAPE
                     HDQSGTSHSISLHSPLRVSRQGERRFGVVGTPGDCVVMGVRHLMRDAPPTLILSGINR
                     GGNLGVETMFSGTVGAAMTGLLLGLPSFALSQTFSDRDKVRWDTARALAPGVIRQLLA
                     IGHDAPTCLNINFPDVDAAAAGPLTPSKQGVGLVEDIEVLPQVDPRGIAYHWLRFQRG
                     TRANDPDSETAVVASGRVSVTPLAFDRTDEPTFARLAASLR"
     gene            complement(3054..4043)
                     /locus_tag="VHV99_13270"
     CDS             complement(3054..4043)
                     /locus_tag="VHV99_13270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636437.1"
                     /translation="MVSESRKVVLMGLLVGAVAIAAYISQAHKSWLSSDEFGLERDSG
                     AAHQTRSDTMTGSVSSGPVAGRSGSAAAIAGDLQAARDSLRRNDLASAQAQLDAVRAV
                     HRDDDQILALQREVAARAELVQHAPVPAQGEQGEKVARQASKSARSSLASSGKSGRSH
                     ESYVARHERANRGSVEAEFRRGTETAGAAVGAVRSSQGRAGGAGAPVVASSSSSGLAD
                     VKVVSKVTSMAAASQATQAEQPVQPARPTSALPPGSQAELTAQTAAAPSSSSPQIVSS
                     AGTQLKSEGGPKTRAQVRAEIARARADGSLPAFGNPDPAGPGGAPSLTTAPRP"
     gene            complement(4413..>4660)
                     /locus_tag="VHV99_13275"
     CDS             complement(4413..>4660)
                     /locus_tag="VHV99_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016355532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX3636438.1"
                     /translation="ARGNGTPQSDSNLATAVRQALDKAPGFNVSGVFVRAQDGAVILS
                     GTVHSADQIRQAEQIARAVPGVLVVVNKLTLWHGGNG"
BASE COUNT          839 a         1509 c         1435 g          877 t
ORIGIN      
        1 aaactgttgt tcaggcagat tgcctgactc acgtacgaga gcggatcgcg attcgtcgag
       61 ttgggacccg gactcgcatc gacatccaca taaaccagcg tgcagggaaa aagattcacg
      121 ttgttcctca caaggcgtga gagacggttc gctttttcca gcgctgccgt tatcgttcgc
      181 ggatcgatcc gcatgtcctt tcgtgtggca agccagaagc tcgtgcgagg ggcgctgccg
      241 tcatgccgcg gctcgcgcct tcatggcgtc acgctcgagc cgggcgtgct gatctgcggc
      301 gatggagtcg gatgaaccgg ggcgcaggta tcaggtatga gctatcgttt cacgtccttg
      361 cgttgccttt ccgccaaaag ccagcgtgac gcaacccggc tgccgtcatt cggctttcgg
      421 cctgtcggaa gtacgcgaca aaatgagtgt tttagagggt acagctccgc tcgaaaacgc
      481 ccgtcttcga cctcggccct catgtcgccg cgagggccga gatgatgcgc acgtcgactt
      541 cgatcgaacc cgtgccatcc aggcggtcga cggtgctttc ctgcagaaaa cgcagcaggc
      601 tcgcctgacg ctcgaacggc agatcgccga tttcgtcaag aaacagcgtg ccgccgtgag
      661 cggcttcgac gcgccctatt ccgcgttgac tcgcgcggcc cggcggcgtc gatatcggat
      721 cggcgccgtg tgccccggcg cagttgcgtc gtctagcgcc gcaactttct gaaaaacttg
      781 cgaatctcgg cggcgagtaa gtcgggcttt tccatcgcgg cgaaatgacc gccgctcggc
      841 atgtcggtcc attgcacgac gttgaaggtc cgctcgagcc acgtgcgcgg cggatgatgg
      901 atttccttgg gaaaacgggc gaaccccacc ggcggcacga tgcgttgccc gtcggtgaag
      961 cgcattggct gcaagcggtt ttcccaatac atctgcattg ccgtgccggc actctgcgtg
     1021 tgccagtaga gcgaaatgtt ggtaagcaat tcgtctttcg aaaatacccg ctcgatgtcg
     1081 ccgtcgcagt cgctccacgc gcgaaacttc tcgccgatcc aggcggcgag tcccacgggc
     1141 gaatcgttca gcgaagccgc cacggtctgc ggttttgtgg cgtgcatgtg ggcatagccg
     1201 ccctcgagcg cagcccattc gttcttctcc cgcagatagc tttcctctgc gggcgtgagc
     1261 ggcgtctgcg tggcgtcatt cgcgggctcg tatgaactcg gcaagaagtt gagatgaatg
     1321 ccgtcgacca cgtcgtgatg tcgagccgcg agcgcgacgg agacgccggc gcccagatcg
     1381 ccgccttggg caccgaaatg ctcgtagcca aggccgcgca ttaacgaagc ccacagatcc
     1441 gccacctgaa acgcggacgt accgggctcg acgggcgcct gcgagaacgc gaaaccgggc
     1501 aacgaaggaa cgacgacgtc gaacgcatcg gccggatcgc aaccgaacgc agacgggtcg
     1561 catagccgct cgagcaaggc gtggaactcg aaaaacgagc ccggccagcc atgcgtgacc
     1621 accagtggat aagacgcggg acccacgccg cgacgatgca cgaagtgcac gcgctgacct
     1681 tcgacttcgg agagaaattg cggttgcaga ttcagcctgc gctcagcctc gcgccaatcg
     1741 aaacgatcgg cccagtactg cgcgagttcg cgcagccagg cggaatcggc gccttgctgc
     1801 cagggcggcg acggcgttgc cggtgcccaa cgtgttgcac ggatacgtcg gcgaagatcg
     1861 tcgatgtcag cgtcgggcac agcaatctga aacggctcta tctgcatgtc atttcatccg
     1921 gcacagggtg tgagagagcg ggcgcttcgg attcgatgac acgcgtcacg gagtccggcg
     1981 ggaaggaagg cccgacgcgg ccatgaagta cgcccggagc gggcaccagg cgcaccaagc
     2041 gcacgaagca atctgcatgg tacacaatga gcgcgtgtcc cgttttgcgt tccggcgacc
     2101 tccatgcttg cagcgcgggc ggcgccactc gcatctcaat gcctcagtga aggcatgtct
     2161 catgtgttcc ccctgccgca gcccgtttcg ctacacgccc tgctcaatag ccggataata
     2221 agacttccct taccgatcga gaggagcgtg ttgcgatgtc tgcctatgaa tccaaagtgc
     2281 cgcgggtact gttgaccaac gacgacggta tcgacgcacc cggcctcgcc gtgctcgaag
     2341 cggttgccgc cgaactcgcc cacgaagtct gggtggtcgc gcctgagcac gatcagagcg
     2401 gcacatcgca ttccatcagt ctgcattcgc cgctgcgcgt gagccgccag ggcgagcggc
     2461 gttttggcgt ggtgggtacg ccgggcgatt gcgtcgtgat gggtgtgcgg catctgatgc
     2521 gcgacgcgcc gcctacgctg attctgtccg gcatcaatcg cggcggcaat ctcggggtcg
     2581 agacgatgtt ctcgggcacg gtcggcgccg cgatgacggg tctgttgctt ggattgccgt
     2641 cgtttgcgtt gagccagact ttcagcgacc gcgacaaggt acgctgggac accgcgcgcg
     2701 cactcgcgcc tggcgtgatt cgccagttgc tcgcgatcgg gcacgatgcg cccacctgcc
     2761 tgaacatcaa ttttcccgac gtcgacgccg ccgccgcggg tccgcttacg ccgagcaaac
     2821 agggcgtcgg actcgtcgag gatatcgagg tgctgccgca agtcgatcca cgcggtatcg
     2881 cttatcactg gctgcggttt caacgcggta cgcgcgcgaa tgaccccgat agcgaaaccg
     2941 ccgtggtggc ctctggacgt gtgtcggtta cgccccttgc gttcgatcgc acggatgagc
     3001 ccacttttgc gcggcttgct gcgtcgttgc gctagccgct gatctgcgcc ggttcacgga
     3061 cgcggcgccg tagtcaggct cggcgcaccg ccgggtcctg cggggtccgg attgccgaac
     3121 gcgggaaggc tgccatcggc gcgagcacgg gcgatttccg cgcgtacctg tgcgcgtgtt
     3181 ttcgggccgc cctccgattt caactgcgta ccggcggagg acacgatctg aggcgacgat
     3241 gatgatggcg ccgctgcggt ttgtgcggtc agctcggctt gtgagcctgg cggcagcgct
     3301 gatgtcggcc gtgccggctg caccggttgt tctgcctgcg tcgcctgtga cgcagcagcc
     3361 atgctggtga ccttcgagac gaccttcacg tccgccagcc cagaagatga cgagctggcg
     3421 acaacaggtg cgccggcgcc gcccgctctc ccctgcgaag acctgacggc gcccacagcg
     3481 gctcctgccg tttcagtacc acgccggaat tccgcctcaa cagatccacg attcgcgcgt
     3541 tcgtgcctcg cgacataact ttcatgcgag cggccgcttt tcccggaaga cgccaacgac
     3601 gagcgtgccg atttcgatgc ttgccgcgcc accttctccc cttgctcccc ttgcgcgggt
     3661 actggcgcgt gctgcaccaa ctccgcgcgc gccgccactt ctcgttgcag cgcgagaatc
     3721 tgatcgtcgt cccggtgcac cgcacgcacc gcgtccagtt gcgcttgggc ggaggcaaga
     3781 tcattgcgcc gcaaactatc gcgcgccgcc tggagatcac cggcgattgc cgccgccgaa
     3841 ccgctacgtc cagcaaccgg tccggaactc accgagcccg tcatagtgtc gctgcgtgtc
     3901 tggtgagccg ccccactgtc gcgctccagg ccgaattcgt ccgacgacaa ccagcttttg
     3961 tgcgcctgcg aaatataagc cgcaatcgca accgcgccga ccaacaggcc catgagaact
     4021 accttcctgg attcgctaac catgtcgtct ccctcgcaat tcgtgaccct gcgcgctcgc
     4081 gaaatcgcaa aacgtgcccg aattcatcaa agggttcacg gcgaatccat ccatcgatgc
     4141 cgccgtaacg accgcgagtt cgggccggta cagtcacgta cgaataagag tgcaaggcgg
     4201 atgcctgcta ttggcgattt ttagactcga aagttcgatt cgtccgctcg cgcggggcgt
     4261 gttgtccgaa aaagccagtt tgccgacccg ccattcctac gacgattttt ctaaactccg
     4321 aagcaatgtc ggacgcaaag accgcggcca ccaattcagc atgacgcacg cagagcgtgt
     4381 ccacgctctc tgtgtaaccg caaaacacga ggtcagccgt tgccgccgtg ccacagcgta
     4441 agcttgttca ccacgacaag cacacccggc acggctcggg cgatctgctc cgcttgccgg
     4501 atctgatcgg cgctgtgcac cgtgccgctc aagatcaccg cgccgtcctg cgcgcgcacg
     4561 aacacgcccg acacattgaa gcccggggcc ttatcgagcg cttgccggac tgccgttgcc
     4621 agattgctgt ccgactgcgg cgtgccattg ccacgtgcca
//
LOCUS       DATASH010000481         6476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4626479,
            whole genome shotgun sequence.
ACCESSION   DATASH010000481 DATASH010000000
VERSION     DATASH010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6476
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4626479"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..665)
                     /locus_tag="VHV99_13280"
     CDS             complement(42..665)
                     /locus_tag="VHV99_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX3636439.1"
                     /translation="MLQILGKASSINVRKVLWACAELNLPFEREDWGSGFKATDAPEF
                     VALNPNAMVPVIRDGELVLWESNSIIRYLAGRYDGEFLYPGDPCERAKCDQWVDWQAS
                     EMNRAWSYAFLALVRRSPAHQDRQQIALSCANWAKHIAIVDRQLETTGAFIAGPKFSL
                     ADMPIALSINRWLETPLRHADFPALTAYMERLAARPGYQEYCRNGTP"
     gene            complement(825..1856)
                     /locus_tag="VHV99_13285"
     CDS             complement(825..1856)
                     /locus_tag="VHV99_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEX3636440.1"
                     /translation="MLTVYSDRHRLHHGHAELIDGEMKPCFEMPSRADFILERVRETR
                     LGEVIAPASFGLDPVHRIHDKRFIAFLGSAWEEWSALGRKHDALPLVWPVRGMRQDRV
                     PEDIDGKLGYYAMDAGVPITSGTWTAVREAVNVALTGARHVSEGARGAFALCRPPGHH
                     AAADYMGGYCYINNAAVAAQSLLDAGAKRVAILDVDYHHGNGTQSIFYHRPDVLFASL
                     HGDPRTEYPFFLGHADETGTAAGLGYNANFPLKPGTAWADYAVALDAACARVADYAPD
                     ALVVSLGVDTFEEDPISQFKLRSDDYPRIGEAIGRLGKPTLFVMEGGYAVAAIGVNAV
                     NVLTGFESV"
     gene            2190..2966
                     /gene="tam"
                     /locus_tag="VHV99_13290"
     CDS             2190..2966
                     /gene="tam"
                     /locus_tag="VHV99_13290"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HEX3636441.1"
                     /translation="MTSSTNWNAKQYVLFESERTRPVRDLLAAVPATEVRTAVDLGCG
                     PGNSTEALAARLPGATVSGLDSSADMIEAARKRLPQFQFDVSDITTWAPTASYDLILA
                     NAVLQWVPDHEQVFASLVNKLAPGGNLAVQMPDNLDEPAHRLLREIASDGPWADKLKG
                     VERTMRHGADWYYTRLVPLCARVDVWRTIYYHPLAGGADAVVEWFKGSALRPFLDALD
                     DSEKAPFLQRYRDAVARAYPALADGTVLLPFPRLFIVATR"
     gene            3130..3495
                     /locus_tag="VHV99_13295"
     CDS             3130..3495
                     /locus_tag="VHV99_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636442.1"
                     /translation="MSDVRHHLSPRDRLELLCWLTCGCLGAYYLNEDWPDAAFHVQAA
                     HKWLDRRSREADWLSIARLSATALEIARRHAKFVDADWARDAVEEILDTDELDPQARL
                     VKQVLADCQSALADKRLAD"
     gene            3660..4595
                     /locus_tag="VHV99_13300"
     CDS             3660..4595
                     /locus_tag="VHV99_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX3636443.1"
                     /translation="MPALNALKAFEVAGRTGSFTRAAELLNVTQSAVSRQVRQLEAQL
                     GETLLQRHHHHLELSAAGRVILQALQQSFDRIELTVRSLQEKTHRNRLRVNAPPTFTS
                     RWLMPRLGRLRDAHPHLELSLSTRVDDKLAESGVLDCAIRFGNGEWEGFDNRLLMNER
                     HIAVCAPALLARQAGRADIDLNQFTLLHVLANADQRYLTWQHWLKAAGIKNVDTRGGY
                     EFDLLDHAIRAAIDGLGVTIADRHMIGRELAEGQLVQVLNVHVDGHQSYWLVTRAEQD
                     ELPHVALFRDWLHQEIWLTERAVDSSKSAQLGEAA"
     gene            complement(4763..6424)
                     /locus_tag="VHV99_13305"
     CDS             complement(4763..6424)
                     /locus_tag="VHV99_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX3636444.1"
                     /translation="MNYDYIIVGAGSAGCILANRLSASGQHSVLLLEAGGKDSSFWFK
                     IPVGFTRTYYNETYNWMYYSEPEKELGNRPIYCPRGKVQGGSGSINAMIYVRGQPHDF
                     DDWARAGNKGWTFRDVLPYFRKLESHPLGNTEYHGADGPIRISPMKDAVHPICRLFLK
                     GCDQAGYPLSDDFNGAQFEGAGIYDVNTRNGQRSSSSFEYLHPVLTRKNLTVERDVLA
                     SRVLFDGNQRAIGVSVTQNGAARQFMANREVILSAGAVDSPKLLQLSGVGDCALLTKH
                     RIAMVKELPAVGQNLQDHLCVSFYYRANVKTLNDEMRPLLGKLKLGLQYLLTRKGPLA
                     MSVNQSGGFFRGHDREVQPNLQLYFNPLSYRIPKSNKANLEPEPYSGFLLAFNPCRPT
                     SRGSIEIASNRAEDAAKIRINALTTEKDIDEVIQGCELVRKVMASPALKEITLEEISP
                     GPQVNTREGFLAYFREQSGSIYHLCGSCAMGDDPRTSVVDARLRVHGIAGLRVVDASI
                     FPNITSGNINAPTMMVAEKGAEMILEDAVADTAFEQEVVALADVV"
BASE COUNT         1259 a         2043 c         1936 g         1238 t
ORIGIN      
        1 tacgatctta tagtgtgacg aatagagcac gaatgcgctg ttcacggcgt gccgttgcgg
       61 cagtactcct ggtagcccgg gcgcgcggca agacgctcca tgtaagccgt cagtgctgga
      121 aaatcggcgt gtcgcaaagg cgtctcgagc caacggttga tcgacagcgc gatcggcatg
      181 tccgcgagcg aaaactttgg tccagcgatg aaggcaccgg ttgtctccag ttgcctgtcg
      241 acgatcgcaa tatgctttgc ccagttcgcg cacgacagcg cgatctgctg acggtcctga
      301 tgagccggcg agcgccggac cagcgcaaga aacgcgtaac tccatgcccg gttcatctcg
      361 cttgcctgcc agtcgaccca ttgatcgcac ttcgcgcgct cacaaggatc gcccggatag
      421 agaaactcgc cgtcatagcg gccagccaga taacggatga tcgaattgga ttcccacagc
      481 acgagctcac cgtccctgat caccggaacc atcgcgttgg gattcaacgc gacgaactca
      541 ggcgcgtcag tcgccttgaa tccggaaccc caatcttccc gttcaaatgg caggttgagt
      601 tcggcacatg cccacagtac tttgcggacg ttgatggatg aggctttacc tagtatctgg
      661 agcatgggat ggtggccgct cgttcagtcg ctgtagataa tttcaccagg aatccggcta
      721 cgaatcgcag ggtatcgatg gttcgatcaa gcggacagat acagttttag tccttcgaat
      781 tgggccaact cgccgaactg gagagagcgt tcagcgacgg cgcctcacac cgattcaaac
      841 cccgtcagca cattcaccgc gttgacaccg atcgccgcga ctgcatagcc gccctccatc
      901 acgaacagcg tcggttttcc caatcgcccg atcgcctcgc cgatgcgcgg atagtcgtca
      961 ctgcgcagct tgaactgcga aatcgggtct tcctcgaaag tatcgacgcc gagcgacacc
     1021 accagcgcat ccggcgcgta gtcagcaacg cgcgcgcaag ccgcgtcgag cgccaccgca
     1081 tagtccgccc acgccgtacc cggtttcaag gggaagttcg cgttatagcc gaggcccgca
     1141 gccgtgccgg tttcgtcggc gtggccgaga aagaacggat attcggtgcg cgggtcgcca
     1201 tgcagcgacg cgaacaggac gtccggacga tgatagaaaa tgctctgcgt gccgttgccg
     1261 tggtgatagt cgacatcgag aatcgccacg cgtttcgcgc ccgcatcgag caatgactgc
     1321 gccgcgaccg ccgcgttgtt gatatagcag tagccgccca tatagtccgc cgccgcgtgg
     1381 tgcccaggcg gccggcacaa cgcgaatgca ccgcgcgcgc cttcgctcac gtggcgtgcg
     1441 ccggtcaacg cgacgttcac cgcttcgcgc acggccgtcc aggtgcccga ggtgatcggc
     1501 acccccgcat ccatcgcgta gtagcccagc ttgccgtcga tatcttccgg cacgcggtcc
     1561 tggcgcatgc cgcgtaccgg ccacacgagc ggcagcgcgt cgtgcttgcg gccgagtgcg
     1621 ctccattcct cccaggcgct gccaagaaac gcgatgaagc gcttgtcgtg gatccggtgc
     1681 accggatcga gtccgaatga ggcgggcgcg atcacttcgc cgagccgcgt ttcccgcacc
     1741 cgttccagga tgaaatcggc gcggctcggc atctcgaagc aaggcttcat ttcgccatcg
     1801 atcaattcag cgtggccatg gtgcagccgg tgccgatcgc tatacacagt caacatggat
     1861 tgtctcctcg ggatgctgca gctcgaaacc gctgcgatgc tcaagttcgc cccgtggggg
     1921 cggagcacgc catcgcttgt cgcgaggatg ccatagctcg gcataaattt gatcaaattt
     1981 ccccaaaggc cgattccgtg cgaggatttg atcaaatctc gtcgaacctg ctggcgatga
     2041 gcgcgatccg cgcaacgctc gccgagatat tgaccgaggc cgcctacgat cccatgttcg
     2101 cgcgtgccgg gcgtatcgaa gcaggtctga aaagtgcgag cgcgcgtcat gcggacagcg
     2161 ctaaggcagt caagacaaag gaacgtgaca tgacttcatc aacgaattgg aatgcaaaac
     2221 agtacgtgct gttcgaaagc gagcgcacgc gtccggtcag ggatctgctg gcggcggttc
     2281 cggcaaccga agtgcgtacg gcggtcgacc tcggctgcgg tcccggcaat tccaccgaag
     2341 cgctggcggc gcgcctgccg ggggcaacgg tcagcggtct ggacagttcc gccgacatga
     2401 tcgaggcggc ccgtaagcgg ctgccgcaat tccagttcga cgtgagcgac attacgacgt
     2461 gggcccccac ggcgtcttac gacctgatcc tcgccaacgc cgtgttgcag tgggtgcctg
     2521 atcacgagca ggtgtttgcg tcgcttgtga acaaacttgc gccgggcggc aatctcgccg
     2581 tgcaaatgcc ggacaacctc gatgaacccg cgcaccgtct gctgcgcgaa atcgcgtcgg
     2641 acggaccgtg ggcggacaag ctgaagggcg tggaacgcac catgcgccac ggcgccgact
     2701 ggtactacac gcggctcgtg ccgctgtgcg cgcgggtcga cgtgtggcgc accatctact
     2761 atcacccgct ggcgggcggc gcggacgcgg tggtcgaatg gttcaagggc agcgcgctga
     2821 gacccttcct ggacgcgctc gacgacagtg agaaagcgcc gttcctgcaa cgctaccggg
     2881 atgcggtcgc gcgagcttat ccggctctcg ccgacggcac cgtgctgctg ccgttcccgc
     2941 ggctgtttat cgtcgcgacg cgctagcgga accgcgccgc tgcggcttgc tgcgtggcat
     3001 gcgtggtgcg gagcgcatag ggtgagtcag caattgccgc tcgcgaattc tgcgtgatat
     3061 tgtgtaacgg aagcgggggg gcggcctgcc gtcctcattg gaacgcaaat tacagcagcg
     3121 agcgtcgcca tgagtgatgt acgccatcat ctgagtccac gggaccgttt ggagctgttg
     3181 tgctggctca catgcggctg ccttggcgct tattatctga acgaggactg gcctgacgcg
     3241 gcgtttcacg tgcaggccgc ccataaatgg ctcgaccgcc ggtcgcggga ggcagactgg
     3301 ttgagtatcg ccagattgtc ggcaaccgcg ctggagatcg cgcggcggca cgcgaagttt
     3361 gtcgatgccg attgggcgcg cgacgccgtc gaggaaattc tcgatacgga cgaacttgac
     3421 cctcaggcgc gactggtgaa gcaggtgctg gcggattgcc agagcgcgct ggccgacaag
     3481 cggttggcgg attagcctct tttgcgcgcc gcacgttgag ggttcgccgg gcggcgccgg
     3541 ctgcggcgcg accacgacca cgtcgttcag tccatacgcg cacaccccag gtaaaatacg
     3601 cgggcccccg cgacaccgcg cgtgagccta cctttgggtg atcaatcttg aaacgcaaaa
     3661 tgcccgcgct gaacgcgcta aaagccttcg aagtcgctgg ccgcacgggc agcttcaccc
     3721 gcgccgcgga actgctgaat gtcacgcaaa gcgcggtcag ccggcaagtc cggcaactcg
     3781 aagcgcaact cggtgagacg ctgctgcagc gtcatcatca ccacctcgaa ctctcggcag
     3841 caggccgcgt catcctgcaa gcgctgcaac agtcattcga ccgcatcgaa ctcacggtcc
     3901 gcagcctgca ggaaaaaacc caccgcaatc gcctgcgcgt caacgcccca cccactttca
     3961 cgagccgctg gctgatgccg cgtctagggc gtctgcgtga cgcgcatcca cacctcgagc
     4021 tgagtctgtc cacgcgcgtc gacgacaagc tcgccgaatc cggcgtgctc gattgcgcga
     4081 tccgtttcgg caacggtgag tgggaaggtt tcgacaaccg tctgttaatg aatgagcggc
     4141 atattgccgt ttgcgcaccc gcgttgctcg cgcgccaggc cggccgcgcg gacatcgatc
     4201 tgaatcagtt cacgctgctg cacgtcctcg cgaacgccga tcagcgctat ctgacctggc
     4261 agcactggct gaaggcggcc ggcatcaaaa acgtcgatac tcgcggcggc tatgaattcg
     4321 atctgctcga tcatgcgatt cgcgccgcta ttgacggcct tggcgtgacc atcgccgatc
     4381 gtcacatgat cgggcgtgag ctggcggagg gtcagttggt gcaagtcctg aatgtgcatg
     4441 tcgacggcca tcagtcgtac tggctcgtca cgcgtgcgga acaggacgag ttgccacacg
     4501 tcgcgctgtt tcgcgactgg ctgcaccagg aaatctggct gaccgaacgt gcagtcgatt
     4561 cgtcgaagtc cgcacagttg ggcgaagccg cttgattcgg acgattcgga cgaaattcgc
     4621 ctgaattgca ggtgcctgag tgacccatgg ttccatttga atcacgtcag agccgtcctg
     4681 cgtgacgcaa aaaaaacgcg ccgattgcat cgcaccagcg cgttttttct gcctatacct
     4741 tctacctgca actccccacc cgtcagacaa cgtcggccag cgccaccact tcctgttcaa
     4801 aggcagtgtc ggcaacggcg tcctcgagaa tcatctcggc gcccttctcc gccaccatca
     4861 tcgtcggcgc attgatgttg ccggaggtga tgttcgggaa gatcgacgca tcgaccacgc
     4921 gcaagccggc aatgccgtgc actcgcaaac gcgcatccac caccgaggtg cgcggatcgt
     4981 cacccatcgc gcaagaaccg cacaagtgat agatcgaacc cgattgctcg cggaaatacg
     5041 ccaggaagcc ttcgcgcgta ttcacctgcg gccccggcga aatctcctcg agcgtaatct
     5101 ctttcaatgc cggcgatgcc atcaccttgc gcaccagttc acaaccctgg atcacttcgt
     5161 cgatatcctt ctccgtagtc aacgcgttga tgcggatttt cgcggcgtct tccgcacggt
     5221 tcgacgcgat ctcgatcgat ccgcggctcg tcggccggca cggattgaat gcgagcagaa
     5281 agcccgaata cggttcaggt tcgagattcg ccttgttgct tttcggaatc cggtatgaca
     5341 gcggattgaa gtacaactga agattcggct gcacttcccg atcatggccc ctgaagaacc
     5401 cgccggactg attcacgctc atcgcgagcg gccccttgcg cgtcaataga tactgcaggc
     5461 cgagcttgag cttgccgagc aacggtcgca tctcgtcgtt cagcgtcttc acgttcgcgc
     5521 gatagtagaa actcacgcac agatgatcct gcagattctg ccccacagcg ggcagttcct
     5581 tgaccatcgc gatgcgatgc ttcgtcagca acgcgcaatc gcccacgccc gagagttgca
     5641 gcagtttcgg cgaatcgacc gcgcccgccg acagaatcac ctcccggttt gccatgaact
     5701 gccgtgccgc gccgttctgc gtcacgctca cgccaattgc ccgttggttg ccgtcgaata
     5761 gaacgcggct tgccagcacg tcgcgctcca ccgtgaggtt cttgcgcgtg agcaccggat
     5821 gcagatactc gaagctgctc gacgaacgct gtccgttgcg tgtattcaca tcgtagatgc
     5881 ccgcgccttc aaattgcgcg ccattgaagt cgtcgctaag cgggtagccg gcctggtcgc
     5941 agcccttcag aaataggcgg cagatcggat gcactgcgtc cttcatcggc gagatacgga
     6001 tcgggccgtc cgccccgtga tattccgtgt tgccgagcgg atgcgactcg agcttgcgga
     6061 aatacggcaa cacgtcgcga aacgtccagc ccttgttgcc cgctctggcc cagtcgtcga
     6121 aatcatgcgg ctggccgcgt acgtagatca tcgcgttgat cgaaccggag ccgccctgca
     6181 ccttgccgcg cggacaatag atcggccggt tgccgagttc cttttcgggc tcgctgtaat
     6241 acatccagtt gtaggtttcg ttgtagtacg tcctggtgaa accgaccgga atcttgaacc
     6301 agaacgagct gtccttgccg cctgcttcga gcagcagcac cgaatgttgg cccgacgccg
     6361 aaaggcgatt ggcgagaatg cagcccgccg aacccgcgcc gacgatgatg taatcgtagt
     6421 tcatgctcta tgtcgccaga cggcgctcaa tccattgaaa cgtagtggtg aaaccc
//
LOCUS       DATASH010000482         4827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3834206,
            whole genome shotgun sequence.
ACCESSION   DATASH010000482 DATASH010000000
VERSION     DATASH010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4827
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3834206"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /gene="rplI"
                     /locus_tag="VHV99_13310"
     CDS             complement(<1..314)
                     /gene="rplI"
                     /locus_tag="VHV99_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX3636445.1"
                     /translation="MQIILLEKVVNLGNLGDIVKVKDGYARNFLIPNKQARRATKEAL
                     AEFEVRRAELEKVAAEKLAAAQAQGEKLAGSTIQIGQKAGVDGRLFGSVTNADIADAL
                     VK"
     gene            complement(342..617)
                     /gene="rpsR"
                     /locus_tag="VHV99_13315"
     CDS             complement(342..617)
                     /gene="rpsR"
                     /locus_tag="VHV99_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEX3636446.1"
                     /translation="MPRPTGKKFDKRRQQQNPLFKRKKFCRFTAAGVDHIDYKDLETL
                     KDFIGENGKITPARLTGTKSHYQRQLDTAIKRARFLALVPYTDQHKA"
     gene            complement(620..919)
                     /gene="priB"
                     /locus_tag="VHV99_13320"
     CDS             complement(620..919)
                     /gene="priB"
                     /locus_tag="VHV99_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012328667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HEX3636447.1"
                     /translation="MNRLQLMASVVEREPVRYTPAGVPIAGCTLHHRTEVVEAGIARQ
                     VELTMQAVAAGEASGKLEGCQMGVETLFTGFLAKKHRNARTLVFHITELQDIGKD"
     gene            complement(970..1343)
                     /gene="rpsF"
                     /locus_tag="VHV99_13325"
                     /pseudo
     CDS             complement(970..1343)
                     /gene="rpsF"
                     /locus_tag="VHV99_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103105.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
     gene            complement(1531..2493)
                     /locus_tag="VHV99_13330"
     CDS             complement(1531..2493)
                     /locus_tag="VHV99_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX3636448.1"
                     /translation="MDCNEARPLLDASADRELPAPDALRVQQHIERCEACRRESEGLQ
                     ALSRSLTAAPYHRAPDALRARIVAALPPTEEAVPALEPRKTAPGQRRWFAWLDSWRGL
                     QGGSRGGTGGMGALGGRGGLGGGLGTAGGGSSRLITQGWLAALVIALGAAAAGVTLSL
                     HRAPEISPFTDELVESHVRAQVSGRDIDVISTDKHTVKPWFNGRLDYSPPVEDLAASG
                     FALEGGRLDYLAHQRVAVLVYRYRKHVIDVYVFPQAGADGTGRGPDGAAPAVLAREGY
                     SIAHWNAEGMTWWAITDAAPDALSGLETALKARLQSGSERTEGG"
     gene            complement(2483..3337)
                     /locus_tag="VHV99_13335"
     CDS             complement(2483..3337)
                     /locus_tag="VHV99_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018432102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX3636449.1"
                     /translation="MVQTQATAARAQPTAETEAARSCRFQQLALPHLDAAYNLARWLC
                     GNSNDADDVVQEAFMRAFRFFDTFRGDSARPWLLAIVRRTWYTEWRRRASSHEVVEFD
                     DSMDDATFDGWSAGGDDPQALLIRGEDAKRVHEALAQLPVEYREVLILRELEEMGYRE
                     IALVADVPIGTVMSRLARGRRKLAALLTEPADGQSGSGSGAGRPGSGRPARDVPQQSH
                     HGPADPRDAPRAAGEGRSESGGAARSPTCPGPTASVTPLRAVSNARSPNTPGGAAAGF
                     AQETPDGL"
     gene            complement(3602..4516)
                     /locus_tag="VHV99_13340"
     CDS             complement(3602..4516)
                     /locus_tag="VHV99_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3636450.1"
                     /translation="MDRFKQIETFVRVADAGSLAAAALEEGVSPVILGRRIDALEKRL
                     GVKLMYRSTRRLVVSEDGAAFLERCRGLLTEWDQAENELSAGRRAVNGHLIVSAPAAF
                     GRKHVAPLAPVFLADKPELQVSFNLTDRVVDLVREGYDLSIRIGGAVDPNFVAVKLAS
                     NRRVVCGTPAYFRQYGKPKTLEDLPQHNCLAFNLQGGQNRGWYFRRNGKLATVRVGGT
                     LDCNDGELLHRWVSEGLGLGWRSTWEIQQQLARGELETVLDEFALPDYDILAVYPQQR
                     YVPAKVRYFIDYLKEVYASEDYWNRPTY"
BASE COUNT          802 a         1616 c         1459 g          950 t
ORIGIN      
        1 tgcttgacca gtgcgtcagc gatgtcggcg ttcgtcaccg agccgaacag acggccgtcg
       61 acgccagcct tctggccgat ctgaatcgtc gagcctgcca gcttttcgcc ttgagcttga
      121 gcggcagcca gcttttcagc ggcgaccttt tcgagttctg cgcggcgcac ttcgaattca
      181 gccagggctt ccttcgttgc acggcgagct tgcttgttcg ggatcaggaa gttacgtgcg
      241 taaccgtcct tgaccttcac gatatcgccc aggttgccca gattgacgac tttttccaga
      301 agaatgattt gcattcagat gctccttatc gcgtcgtcgg gttaggcctt gtgctggtcg
      361 gtgtacggca ccagcgcgag gaaacgtgcg cgcttgattg ccgtatccag ctggcgttga
      421 tagtgcgact tcgtacccgt gagacgcgcc ggcgtgatct tgccgttttc gccgatgaag
      481 tccttcagcg tttcgaggtc cttgtagtcg atgtgatcga cgccagcggc cgtgaaacgg
      541 cagaacttct tgcgcttgaa gagcggattt tgttgctgac gacgcttgtc gaatttctta
      601 ccagtcgggc ggggcatgtt cagtcctttc caatgtcctg caattctgtg atgtgaaata
      661 ccagagttct tgcgttgcgg tgctttttcg ccagaaagcc tgtgaagagc gtttccacgc
      721 ccatctgaca gccttccagc ttaccgctcg cctcgcctgc ggctaccgcc tgcatggtca
      781 gttcgacttg ccgggcgatg ccggcttcga cgacttccgt gcggtggtgc aacgtgcagc
      841 ctgcaatcgg aacgccggcg ggggtatacc gcaccggttc gcgttcgacg acgctggcca
      901 taagttgcag ccggttcatg tagccttcga cgaaaacgac gcttgatctg gtagcttaaa
      961 tagtgtgtct taagcctgcg cttcggacgg ttgcgtagca gccgacttct tggcttcttc
     1021 gcgctgcact tccttcatca tcggcgacgg gccggtttcg gccttctcat cttgacgata
     1081 aggtgacgca gaacagcgtc gttgaacttg aatgcgtgtt ccagctcgtc gagcgtggtt
     1141 tggtcgcatt cgatgttcat gcagacgtag tgagccttcg cgagtttctc gatcatgtag
     1201 gccagttggc gacggcccca gtcttcgata cggtggacct ggccaccgtg cgaggtgatc
     1261 gtgctcttgt aacgctcgat cattgcgggc acctgctcgc tttgatccgg atgcacgata
     1321 aatacgattt cgtaatgacg catattcact ccttgtggat taaagccacc cgggcgtcgg
     1381 aaccggtgtg gcaagtgaga agccaaagag tgtaacccga atggggccgg gttgcaagat
     1441 atcccgttaa tcgggcgcgc gattcaggag tgttttcaag ggtttaggtc gtggcggggc
     1501 gaggcagccg gcggccctca ccccaatccg tcagccgcct tccgtgcgct cgctgccgct
     1561 ttgcaaacgc gccttcaacg ccgtttccag cccgctcaac gcatccggcg cggcgtccgt
     1621 gatcgcccac caggtcatgc cctcagcgtt ccagtgcgcg atcgaatagc cttcgcgggc
     1681 aagcacggcg ggcgccgcgc cgtccgggcc gcgccccgta ccgtcggcgc cggcctgcgg
     1741 aaatacatac acgtcgatca catgcttgcg atagcggtac accagcaccg ccactcgctg
     1801 atgcgcgagg taatccagcc gcccgccctc cagcgcgaag ccgctggcgg ccagatcttc
     1861 gaccggtggc gaatagtcga gccggccgtt gaaccacggc ttgacggtat gtttgtccgt
     1921 cgaaatcacg tcgatgtcgc ggccggacac ctgcgcccgc acatggcttt ccaccagttc
     1981 gtcggtgaac ggcgaaatct ccggtgcccg gtgcaggctc agcgtgacgc ccgcagcagc
     2041 cgcgccgaga gcaatcacca gcgccgccag ccagccttgc gtaatcaacc ggctcgaccc
     2101 gccaccggcg gtgcccagcc ctcctcccag gccgcctcgg ccgccgagag cgcccatacc
     2161 gcccgtaccg ccccgcgagc caccctgcag gccgcgccag ctgtccagcc aggcgaacca
     2221 gcgccgctgg cccggtgccg tcttgcgagg ttcgagcgcc gggacggcct cttccgttgg
     2281 cggcagcgcc gcgacgatcc gggcccgcag cgcgtccggc gcgcggtgat aaggcgcggc
     2341 ggtcagcgac cggctcagcg cctgcaagcc ctcgctttcc cggcggcaag cctcgcaacg
     2401 ctcgatgtgc tgctgcaccc gcagcgcatc cggcgcgggt aattcacgat cggcgctcgc
     2461 gtcgaggagc ggccgcgctt cgttacagtc catctggcgt ctcctgagcg aagccggcgg
     2521 cagcgccgcc aggcgtgttg ggtgaccggg cgttcgagac cgcccggagc ggtgtaaccg
     2581 aggccgttgg ccccgggcat gttggcgagc gtgcggcgcc gccggactcc gacctgccct
     2641 cgcccgcggc gcggggagcg tcgcgtggat cggccggacc atgatgcgat tgctgtggca
     2701 cgtcgcgcgc cggacgccca gaccccgggc gccctgcccc tgatccagac ccagactgcc
     2761 cgtctgccgg ctctgtcagg agcgccgcca acttgcggcg cccgcgtgcg agcctcgaca
     2821 tgactgttcc gatcggcacg tcggcaacca gtgcgatctc gcgatagccc atctcttcga
     2881 gttcgcgcag gatcaggacc tcgcggtact ccaccggcag ctgcgcgagc gcctcatgca
     2941 cccgcttcgc gtcttcgccg cgaatcagca gcgcctgcgg atcgtcgccg ccggcgctcc
     3001 agccgtcgaa agtcgcgtcg tccatgctgt cgtcgaactc caccacctcg tgcgacgacg
     3061 cgcgtcgccg ccattccgtg taccaggtgc ggcgcacgat cgcgagcagc cacggccgcg
     3121 ccgagtcgcc acggaaagtg tcgaagaaac ggaacgcacg catgaacgct tcctgcacga
     3181 cgtcgtccgc atcgttcgag ttgccgcata gccagcgcgc gaggttgtac gccgcgtcga
     3241 ggtgcggcag tgccaactgc tgaaaacggc agctcctcgc cgcctcggtt tcggccgttg
     3301 gctgcgcgcg ggctgcggtt gcttgggtct ggaccacctc ggccctccag ggtattgcgg
     3361 ctaggctgtg tgacgcgttt tcactcgctc accacgcata accgctcaca tgccgagttt
     3421 attcccgtct gcgtcgtcaa tccgaacata taagagggaa tacgctggcg cgccgcgccg
     3481 atgagcgggc ggacacgcac aatttgaaga cgaggccgtc gaacatttcc atcgccatcg
     3541 cgggttgcca cgcgccgccg ggcgcatcca gccccagcgc agtagccgcc cacgccagac
     3601 actaataagt cgggcgattc caataatcct cgctggcata cacctctttc agataatcga
     3661 tgaagtagcg caccttggcc ggcacataac gctgctgcgg gtagaccgcc agaatgtcgt
     3721 agtcgggcag cgcgaattca tccagcacgg tttccagttc accacgcgcc agttgctgct
     3781 ggatttccca cgtggagcgc cagccgagcc cgagcccttc ggagacccaa cgatgcagca
     3841 actcgccgtc gttgcaatcg agcgtgccgc ccacccgcac cgtcgccagt ttgccgttgc
     3901 ggcggaagta ccagccgcgg ttctgtccgc cctgcaggtt gaacgccagg cagttgtgct
     3961 gcggcagatc ctcgagcgtt ttcggtttgc cgtattggcg gaaatatgcc ggcgtgccgc
     4021 acaccacgcg ccggttcgac gccagcttca ccgcgacgaa attcggatcg accgcgccgc
     4081 cgatccggat cgacaggtca taaccctcgc gcaccagatc cacgacccgg tcggtcaggt
     4141 tgaacgacac ctgcaattca ggcttgtcgg caagaaaaac cggcgcaagc ggtgcgacgt
     4201 gcttgcgccc gaacgcggcc ggcgccgaca cgatcagatg gccgttcacc gcgcgccgtc
     4261 cggcgctcag ttcgttttcc gcctggtccc actcggtcag gaggccgcgg cagcgctcga
     4321 gaaatgccgc gccgtcttcg ctgactacga ggcgccgcgt cgagcggtac atcagcttca
     4381 cgccgaggcg cttttccagc gcgtcgatgc ggcgtccgag aatcacgggc gacacgccct
     4441 cttcgagcgc cgctgcggcg aggctgccgg cgtcggcgac gcgcacgaag gtttcgatct
     4501 gtttgaagcg gtccattttt gtctcctact cgtcgcgcct tgagatgaat ggcttgaggg
     4561 ccactgctgg ctcgagggtc actgcctggc ttctggctcg cagcctggaa cccggatctc
     4621 gccccgcttc accagccccg gcgctcaggc ctcagacacc gaatccactg gtccgctctt
     4681 cgcggccacc cgccattcca tactttttgt ttcgaaataa gcgacccgga ctgatcttat
     4741 caaaccttta ggtgaagcct acagtttgat caacacctct ggtttgcata tcccgtccgc
     4801 gaattcgtca gccaacccat tcgccca
//
LOCUS       DATASH010000483         4466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6009290,
            whole genome shotgun sequence.
ACCESSION   DATASH010000483 DATASH010000000
VERSION     DATASH010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6009290"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1375)
                     /locus_tag="VHV99_13345"
     CDS             complement(<1..1375)
                     /locus_tag="VHV99_13345"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HEX3636451.1"
                     /translation="MNHPSFEGKPTIEAATGAPAWVKHQKLIDWVQRIAAMTKPDRIV
                     WCDGSQEEYDRLCAQMVEAGTLKKLDSAKRPNSYLAWSDPSDVARVEDRTYICSQRRE
                     DAGPTNNWIEPVEMRSTLDGLFDGAMRGRTLYVVPFSMGPLGSPIAHIGVELSDSPYV
                     VTNMRIMTRMGRKVYEVLGEDGEFVPCVHSVGAPLAAGAKDVPWPCNETKYIVHFPES
                     REIWSYGSGYGGNALLGKKCFALRIASTMGRDEGWLAEHMLILGVTSPEGRKHHVAAA
                     FPSACGKTNFAMLIPPEGLNGWKISTIGDDIAWIKPGKDGRLYAINPEAGYFGVAPGT
                     SEKTNFNAMATLKENVIFTNVALTDAGDVWWEGMTDEPPAHLIDWQGKDWTPAGAKES
                     GRKAAHPNARFTAPASQCPSVDPDWENPAGVAIEAFIFGGRRSTTVPLVTEARNWVEG
                     VYMAATMG"
     gene            1753..2766
                     /locus_tag="VHV99_13350"
     CDS             1753..2766
                     /locus_tag="VHV99_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004188904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HEX3636452.1"
                     /translation="MKIAILDDYQDAVRKLDCFHLLADHEVKVFNNTVRGLGQLASRL
                     SEVDALVLIRERTRISSQLLDKLPRLRMISQTGKVSSHIDLAACTERGIAVLEGSGSP
                     IAPAELTWALIMAAQRRIPQYVANLKQGAWQQSGLKTSALPPNFGLGQVLRGQTLGIW
                     GYGKIGRLLAGYGKAFGMNVLIWGREHSREAARADGYSVAESREALFEQSDVLSLHLR
                     LHDDTRGIVTQEDLMRMKPTALLVNTSRAELLEENALVTALSHNRPGMVAIDVYESEP
                     ILQGYSLLRMENVICTPHIGYVERESYELYFTAAFQNILAFDAGDTASVANPEALQGG
                     RRR"
     gene            complement(2878..3753)
                     /locus_tag="VHV99_13355"
     CDS             complement(2878..3753)
                     /locus_tag="VHV99_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX3636453.1"
                     /translation="MFDQVVFAGGGNRCWWQAGFWDVVQPELAIRPRVITGISAGAAT
                     ACMLYTRDSDWVMRYYEDALRHNTRNAYWGNLLRGESVFPHYRIYRQALLDIYGARFA
                     TLAQAPEIRIGVSHLPRWLGARSAVAAGLIAYNIEKYVRKTLHPTLGQTLGFHPEFVR
                     AQDCATVEDLADLILQSSSTPPFTPVLRRNGRQVLDGGMVDNVPVGALDADAPGSVLV
                     MVTRLYPRPQMFVVPHGVQQRLYVQPSRKVPISSWDYTSPSQMVHAYNLGRADGEVFL
                     ERVPDLLEAGARETR"
     gene            complement(3806..4210)
                     /locus_tag="VHV99_13360"
     CDS             complement(3806..4210)
                     /locus_tag="VHV99_13360"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX3636454.1"
                     /translation="MDDKEIIALLDRILAPWVRALTLTPVKVDEESATLRLPFSGGLR
                     HAGGVICGQVFMAAADTAMIVAISAALGGFKPMSTVSLNISFMRAVRKGDVLITARVL
                     RMGRNLVFGEVELFDEAGNMAVHATTTYALLD"
BASE COUNT          836 a         1394 c         1387 g          849 t
ORIGIN      
        1 agcccatggt cgccgccatg tagacgcctt ccacccagtt gcgcgcttcg gtgacgagcg
       61 gcacggtggt ggagcggcgt ccgccgaaga taaacgcctc gatcgcgacg cccgccggat
      121 tttcccagtc ggggtcaacg gacgggcatt gcgaggccgg cgccgtaaag cgcgcgttcg
      181 ggtgcgccgc cttgcggccg ctttccttcg cgccggccgg cgtccagtcc ttgccttgcc
      241 agtcgatcag gtgcgcgggc ggttcgtccg tcatgccttc ccaccagacg tcgccggcgt
      301 cggtgagcgc gacgttcgtg aaaatcacgt tttccttcaa cgtggccatc gcgttgaagt
      361 tggtcttttc gctcgtgcct ggcgcgacgc cgaaatagcc cgcttccggg ttgatcgcgt
      421 acagacggcc gtccttgcca ggcttgatcc acgcaatatc gtcgccgatc gtggagattt
      481 tccagccgtt caggccttcc ggtggaatca gcatcgcgaa gttggtcttg ccgcacgccg
      541 acggaaacgc cgccgcgacg tgatgcttgc gcccttccgg cgacgtcacg ccgagaatca
      601 gcatgtgttc agcgagccag ccttcgtcgc ggcccatcgt cgaggcgatg cgcagcgcga
      661 agcacttctt gccgagcagc gcattgccgc cgtagcccga gccgtagctc cagatctcgc
      721 gcgattcagg gaaatgaaca atgtatttgg tctcgttgca cggccacggt acgtctttcg
      781 cgcccgccgc gagcggcgcg ccgaccgaat gcacgcacgg cacgaactca ccgtcttcgc
      841 ccagcacctc gtacactttg cggcccatgc gcgtcatgat gcgcatgttg gtcaccacgt
      901 acgggctgtc gctcaactcg acgccgatat gcgcaatcgg cgaacccagc ggccccatcg
      961 aaaacggcac cacgtacagg gtgcggccgc gcatggcgcc gtcgaacaga ccgtcgagcg
     1021 tcgagcgcat ctcaaccggt tcgatccagt tattagtggg cccggcgtct tcacgacgct
     1081 gcgagcagat ataggtgcgg tcttcgacac gcgccacgtc cgacggatcg gaccatgcca
     1141 gataggaatt gggacgtttg gcggaatcga gcttcttgag ggtgccggct tcgaccatct
     1201 gggcgcatag gcggtcgtac tcttcctgtg aaccatcgca ccagacgatt cgatcaggct
     1261 tggtcatggc ggcaatgcgc tggacccagt cgatcagctt ttggtgtttg acccacgcgg
     1321 gcgcccctgt ggccgcttca atggtgggct ttccttcgaa tgagggatga ttcatcgacc
     1381 tgtctccgtt ttgaaagcaa tagaaaaacg gtacaagccc aaggacgctg catgcgagag
     1441 gcgtttaatt catcacgtcg acattcctgc aagccgacgc gcaaaatgtg caggtcatca
     1501 tgggctacac agcacgttgc gcggaggttt ttgccgaggg ctgtctgcaa ccgtctgtaa
     1561 agcgacttgc ctcaacacgc aacaaactgt taaaaacgat gtcaatcggc cttgccgtgg
     1621 cgctgccgtt ctaagcggca gcttctacag taaggcattc agccagaccg gcatgtgacc
     1681 tgggtcacat ggtgggacag tcagcataac actccgttcc gcaccgctat ggtgcgccgc
     1741 aagacacata ccatgaagat tgccatcctc gacgattacc aggacgccgt ccgcaagctc
     1801 gactgctttc atctgctggc cgaccatgag gtcaaggttt tcaacaatac cgttcgcggt
     1861 ctcggccaat tggcaagccg tctttccgaa gtcgacgcgc tcgtcctgat tcgcgaacgc
     1921 acccgcatca gctcgcaact gctcgacaag ttgccgcgtt tgcgcatgat cagtcagacg
     1981 ggcaaggttt cgagccatat cgatctggcg gcctgtactg agcgcggcat cgccgtgctc
     2041 gaaggcagcg gctcaccgat cgcaccggcc gaactgacgt gggctctcat catggcggct
     2101 cagcggcgga ttccgcaata cgtagcaaac cttaagcaag gagcctggca gcagtccggt
     2161 ttgaagacgt cggcgttgcc gccgaacttc ggcttgggcc aggtattgcg cggtcagacg
     2221 ctgggaattt ggggttacgg caagatcggc cggctgcttg ccggatacgg caaggccttt
     2281 gggatgaacg tgctgatctg gggccgcgag cattcgcgcg aagcggcgcg cgcggatgga
     2341 tatagcgtgg ccgaaagccg tgaggcactg ttcgagcaga gcgacgtact gtcgttgcac
     2401 ctgcgtctgc atgacgatac gcgcggcatc gtcacgcagg aagacctgat gcggatgaag
     2461 ccaaccgcgc tgctcgtcaa caccagccgg gctgaattgc tcgaagaaaa cgcgctggtg
     2521 accgcgttgt cgcacaaccg tcccggaatg gtcgcgatcg atgtctacga gagcgagccg
     2581 attctgcagg gctacagcct gctgcgtatg gagaatgtga tctgcacgcc gcacattggc
     2641 tacgtggagc gcgagagcta cgaattgtat ttcaccgcgg cattccagaa cattctggct
     2701 ttcgacgccg gcgatacggc cagcgtggcg aatccggaag cgttgcaggg cgggcggcgt
     2761 cggtagcacg cggtaggctg cgcggttcga cgcgagcggt ttatccgtcc gcgcagcagt
     2821 gcgttgtcta gcgaagccgc gccggtggcc gccactgcgg cccccgcctg cctcacctca
     2881 gcgcgtttcg cgcgcgccgg cttccagcag atcgggcacg cgctccagaa agacttcgcc
     2941 gtcggcacgg ccgaggttgt acgcgtgcac catctgcgat ggactcgtgt aatcccaact
     3001 cgaaatcggc accttgcgcg acggttgcac gtagagccgt tgctgcacgc catgcggcac
     3061 cacgaacatc tgcggccgcg gataaagacg cgtcaccatc accagcacac tgcccggcgc
     3121 gtcggcatcg agcgcgccga ccggcacgtt gtcgaccatc ccgccgtcca gaacctgccg
     3181 gccgttgcgc cgcagaaccg gcgtgaacgg cggcgtactc gacgattgca gaatcagatc
     3241 ggcgaggtcc tcgaccgtcg cgcaatcctg cgcgcgcacg aattccggat gaaaaccgag
     3301 cgtctgaccg agcgtgggat gcagcgtctt acgcacgtat ttctcgatgt tgtacgcaat
     3361 caggccggcg gccaccgcgc tgcgcgcgcc gagccaacgc ggcagatgcg acacgccgat
     3421 acggatctcg ggcgcttgcg ccaaggtagc gaacctggcg ccgtagatgt cgagcagcgc
     3481 ctgccggtag atccggtaat gcggaaacac cgattcgccg cgcagcaaat tgccccagta
     3541 cgcgttgcgc gtgttgtggc gcagcgcgtc ttcgtaatag cgcatgaccc agtcggaatc
     3601 gcgcgtgtac agcatgcacg cggtggcggc gccggccgaa atgccggtga tgacgcgcgg
     3661 acggatagcc agctctggct gcaccacgtc ccagaatccc gcctgccacc agcagcgatt
     3721 gccgccgccg gcgaaaacga cctgatcgaa catcttggct ttccctctga tgcggcgtgt
     3781 gagcccgctt tttaggtttc gcgtttcagt cgagcagcgc gtacgtagtg gtggcgtgaa
     3841 cggccatgtt gcccgcttcg tcgaataact cgacttcgcc gaacacgaga ttgcggccca
     3901 tgcgcagcac gcgcgcggtg atcagcacat cgcccttgcg aaccgcgcgc atgaaactga
     3961 tgttgagcga caccgtgctc atgggtttga acccgccgag ggccgcggaa atggcgacga
     4021 tcatggccgt gtcggcggcc gccatgaaga cctggccgca gatcacgccg cctgcatggc
     4081 gtagcccgcc ggaaaacggc aggcgcagcg tggcactttc ttcgtcgacc ttgaccgggg
     4141 tcagcgtgag cgcacggacc cacggcgcga ggatgcgatc gagcaacgcg ataatctctt
     4201 tgtcatccat ggcggtcccc ggtcgaaagc cgcacgaggc ttcgggcggc aagtgttcgc
     4261 gacataatac cgggacggcg aaccgcgcgc ccttccggcg cttgactgct ctcagcgcgg
     4321 atgcgaggcc gctgcacttc ccagattttt tcagaaatct gctaaagggg gttggcaatc
     4381 tcggaaagat tcggcataat ttcgcttctg tttggggcgt tagctcagtt ggtagagcag
     4441 cggactctta atccgtaggt cgagtg
//
LOCUS       DATASH010000484         7073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2647569,
            whole genome shotgun sequence.
ACCESSION   DATASH010000484 DATASH010000000
VERSION     DATASH010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7073
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2647569"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="VHV99_13365"
     CDS             <1..579
                     /locus_tag="VHV99_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010098071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate binding domain-containing protein"
                     /protein_id="HEX3636455.1"
                     /translation="LVPLIATFRRRYPLIGLDVTLTERPVDLVADGYDLGVVLPFMLT
                     SDTTVTRLLERIPLVIVVTPGYLEGKQRPTHPSELAELEFVAMHPSIRKPMLTFRLDG
                     GEQIAVPLQADVTSNNAVFNRELVLEGFGLGVLPAALVRDELASGKLVSLLDDFEIVD
                     GAIEIRLAYSSRTLLPAKVRAFIEHAAEFFEA"
     gene            704..1126
                     /locus_tag="VHV99_13370"
     CDS             704..1126
                     /locus_tag="VHV99_13370"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX3636456.1"
                     /translation="MHTRPPRHDDCFAVRQAARHISQLYERHLSAVQITPTQFSIVSA
                     LERMPDMTMAELAQAMVMERTTLVRVLQPLLRNGLVMSTMGGPCQRRLQLTLTEGGRT
                     KLADALRHWQAAQEEFEAKFGRRQAARLRRELFRLTSE"
     gene            1185..2285
                     /locus_tag="VHV99_13375"
     CDS             1185..2285
                     /locus_tag="VHV99_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEX3636457.1"
                     /translation="MTTPSTPLASTAAPSAERGARRPFRLGWMPLAIIAVVVVIVAAA
                     TYWFTVLRFVQSTDDAYVGGNVTVMAPKVNGFVTELLVSDNQRVKANQVLIRLDSRDY
                     DARLAQANADVQGARAAVAELEAKQQLQAAVINQSQAEVRASAAEVTRSASDQVRYRQ
                     LVKDEAVSNQVVERADADYLKARATLDNSNAAALASQRQLAVLAAQIDDARARVATAE
                     ALRRIAELNVEYTTIRSPVDGYVGNRTARVGMLANTGASLLTIVPTDGLWIDANFKED
                     QLRKMHVGDRVEVELDASSGTFQGHVESLAPATGATFSVLPPENATGNFTKIVQRVPV
                     RIRLDVPKDMQNVLRAGLSATVKVHLDSASRS"
     gene            2282..3856
                     /locus_tag="VHV99_13380"
     CDS             2282..3856
                     /locus_tag="VHV99_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014251128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX3636458.1"
                     /translation="MSPAVPLNPADQPTRQRVFAFALMCLGFFMATLDIQIVASSLKD
                     IGGGLSASQDELSWVQTSYLIAEILVIPMSGWLSKVFSTRWLFVASAVGFTITSMLCG
                     LAWDINSMIIFRGLQGAAGAAMIPTVFTTAFVLFPGKQRLIASTTIGALASLAPAIGP
                     VIGGWITSQWSWHWLFYLNLAPGLVVAFLVPKFVRIDEPDLSLLKKGDYLGIALMSGF
                     LGCLEYVLEEGPRKNWFGDDVIITCTWISAICGFLFLVHAFTAKDPVVDLRALAVRNF
                     AIGCLLSFVTGIGIFVAVFLTPLFLSRVRELDSLQIGIALLSVGVFQLLALGAYAFAS
                     RLVDMRWCLLFGLVCFGLGCYFYVPLTNDWGWQQLLLPQALRGIGQQFAVPPIVTMAL
                     GSLPQSRLKSASGLFNLMRNLGGAIGIAVSATMLNDRLNFHYLRISESVTTGSPETEA
                     FLSRQFAHWGSVAGDALNATNAGLANLHAMVFREALVMTFSDTFYVLAMCFAVGIVSV
                     LFVRPIGAAPPSPDAH"
     gene            3865..5268
                     /locus_tag="VHV99_13385"
     CDS             3865..5268
                     /locus_tag="VHV99_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016354522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEX3636459.1"
                     /translation="MYKILLALAASMTLAACAVQPASHADLPSTVQSIAPANWSVDAP
                     QDNVDAAAWWEQFGDPVLHELVSSVLSQNLDVQAALERVKQAQAVTTQRRAALLPELD
                     ASATASDARQNTPPPLGYVRQAGFGLTLGWTPDVFGGERLELLAAQAQLTGRQHDVDA
                     LRLALAANTASAYVDLRWAQSEQKILRDNIAIRERALRLTQERMQYGLSPRLDVTRAQ
                     NQLSELQARLPRADATVQHQLSLIAVYSGRTPESVDTLVLATPGTIPTPSNAVPQMLP
                     SEALLRRPDVRSAYAELTQRAAEVGVAQADRYPKFSLQLTDGLLASSYLGLPTLTDNL
                     FSAALNATSPIFNAGRITAGIEQSQSRMRESDLRLRQTMLQALKDVEDTRTDLVSTSE
                     QAGRLKDALQSSDQSLRLSTQLYKGGASGFLDVLAAQEAYLQDSDALNQARREHALAA
                     VALYRSLGGGWSEAKDE"
     gene            5330..6259
                     /locus_tag="VHV99_13390"
     CDS             5330..6259
                     /locus_tag="VHV99_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3636460.1"
                     /translation="MALKNLLRRLDLTTLQLFLAVYEEGTLTRAAEREAIAVSAASKR
                     LLELEQAVGATLFVRRARGMELTPAGETLLHHARRVLRDVENIGIELAEHATGVRGYV
                     RMMANLSAIVEFLPEDLRAFFSMNERIKLDLEERPSSGVVQGIADSLADVGICSGEAD
                     TRGLDVSHYRHDSLVVVMREDHALAAREQVTFVETLDSDYVGLHAASSINLRTQTAAR
                     QAGKPLRLRIQVPGFDGVCRMVQTGLGISVLPLKVFNTVGRPLGLTAVTLDETWSQRD
                     LIVVVRDVAGLPPLSRMLFDHLRAVEHQEQNKA"
     gene            6364..>7073
                     /locus_tag="VHV99_13395"
     CDS             6364..>7073
                     /locus_tag="VHV99_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX3636461.1"
                     /translation="MAGPLAGIRVIEIGTLIAAPFAARMLGEFGADVIKIEAPDNGDP
                     LRKWRKLHEGTSLWWYLQSRNKKSVCVNLKSPDGVDVVKRLAADADVVIENLRPGALE
                     KLGLGWDVLHAINPKLTMVRISGYGQSGPYRDRPGFGAIGEAMGGIRYTTGEVAGAPA
                     RVGVSLGDSLASLHGVIGALMSILRVKTGQGEGQVVDVSLVESVFNLMESLIPEYDLL
                     GHVRERSGGALPGIAPSNT"
BASE COUNT         1271 a         2151 c         2241 g         1410 t
ORIGIN      
        1 ctggtgcctt tgatcgcgac cttccgccgc cgataccccc tgatcggact cgatgtgacc
       61 ctgaccgagc gtcccgtgga cctggtggcg gacggctacg acctcggtgt ggtgttgccc
      121 ttcatgctga ccagcgacac caccgtcacg cgcctgctcg agcgcattcc gctcgtcatt
      181 gtggtgacgc cggggtatct ggaaggcaag cagcggccga cgcatccgtc cgagctcgcg
      241 gagcttgaat ttgtcgccat gcacccgtca atccgcaagc ccatgctgac gttccggctg
      301 gacggcggcg aacaaatcgc ggtgccgctg caagccgacg tgacatcgaa caacgcggtg
      361 ttcaaccgtg aactggtgct ggagggcttc ggcctgggcg tgctgccggc cgcgctggtt
      421 cgtgacgaac tggcgtcggg caagctggtg tcgctgctgg acgacttcga gatcgtcgac
      481 ggtgcgatcg aaatccgcct ggcttacagc agccgcacct tgctgccggc caaggtaaga
      541 gcgttcatcg aacacgctgc ggaattcttc gaagcctgag cggggcgaac aagctttcat
      601 ttttatttca catcgatttc gtttcgattg ttaacggatt cgacattaat tgatgcgcgt
      661 aatagcggtt gactacgtgc atatgcacac ataatctgtg cacatgcaca cacgcccccc
      721 acgacacgac gactgttttg cggttcgcca ggctgcgcgg cacatctcgc agttatacga
      781 gcggcattta tcggcagtac agatcacgcc aacgcaattc agcattgtct ccgcgcttga
      841 gcgcatgccg gacatgacga tggcggaact cgcgcaggcc atggtgatgg agcgcacgac
      901 gctggtccgg gtgctgcagc cattgctgcg caacgggctt gtcatgtcga cgatgggcgg
      961 accctgccag cggcgtttgc agttgacgct gaccgaaggc ggccggacca agctcgccga
     1021 cgcgctgcgg cactggcagg cagcgcaaga agaattcgag gcgaagttcg ggcgccggca
     1081 agcggcacgt ctgcgccgcg aactgtttcg cctgaccagc gagtgaccag gcaaagcgcc
     1141 acgcaccagc ataacccatt cgatgcaacc aacaggactc accgatgacc acaccttcca
     1201 cgccgcttgc ctccaccgcc gcgccatcgg ctgagcgcgg agcgcggcgg ccttttcgac
     1261 tcggctggat gccgctcgcg atcattgcgg tggtggtcgt gatcgttgct gcggccacat
     1321 actggttcac cgttttgcgt ttcgttcaaa gcacggacga cgcgtatgtc ggcggcaacg
     1381 tgacggtaat ggcgccgaaa gtgaacggtt tcgtcaccga actgctcgtc tccgataacc
     1441 agcgcgtgaa ggcgaaccag gtgctgatcc ggctcgactc acgcgattac gacgcaaggc
     1501 tcgcacaggc aaatgccgac gtgcagggtg cgcgcgccgc tgtcgccgag cttgaagcga
     1561 agcagcagtt gcaggccgcc gtgatcaacc agagccaggc ggaggtgcgg gcatcggcgg
     1621 cggaagtcac gcgcagtgcg tctgaccagg tgcgttaccg gcaactcgtc aaggacgaag
     1681 cggtatcgaa ccaggtggtc gaacgtgccg acgcggatta cctgaaggcc cgcgccacgc
     1741 tcgataacag caacgcagca gcgctcgctt cgcagcgcca actcgccgtg ctggccgcac
     1801 aaatagacga cgcccgtgcg cgtgttgcga ccgctgaagc gttgcggcgc atagccgaac
     1861 tgaacgtgga gtacacgact atccgctcgc ctgtggacgg ctacgtcggc aaccggacgg
     1921 cgcgcgtggg catgctcgcg aacacggggg cgtcgctgct gacaatcgtg cctaccgacg
     1981 gcttgtggat cgacgcaaac ttcaaggaag accagctcag gaagatgcat gtcggcgatc
     2041 gtgtcgaggt cgaactcgac gcctccagcg ggaccttcca gggtcatgtg gaaagcctgg
     2101 cgcccgcgac cggtgcgacc ttcagcgtat tgccaccaga gaacgcaacc ggcaacttca
     2161 cgaagatcgt gcagcgcgtg ccggtgcgga ttcgcctcga cgtaccgaag gacatgcaga
     2221 acgtgctgcg cgccggcctc tcggcgaccg tgaaggtaca tctcgacagc gcgagccgtt
     2281 catgagcccc gccgttccac tgaaccccgc cgatcagccc acaagacagc gtgtatttgc
     2341 gttcgcgctg atgtgcctgg gcttcttcat ggcgacgctg gacatccaga tcgtggcgtc
     2401 gtcgctcaag gatattggcg gcggtttgtc ggctagccag gacgaactct cgtgggtgca
     2461 gacgtcatac ctgattgcgg aaatcctggt gatccccatg tccggctggc tctccaaagt
     2521 gttctccacg cgctggctgt tcgtggcttc cgcggtcggt ttcaccatca cgagcatgct
     2581 gtgcggcctc gcgtgggaca tcaattcgat gatcattttc cgcggcctgc aaggtgcggc
     2641 cggcgctgcg atgatcccga ccgtcttcac tacggcgttc gtgctgtttc ccggcaagca
     2701 gcggctgatt gcatcgacga cgatcggcgc gctcgcttct ctcgcacccg cgatcgggcc
     2761 tgtcatcggc ggctggatca cgtcgcaatg gtcgtggcac tggttgttct acctgaacct
     2821 tgcgccgggg ctcgttgttg cgttcctggt gccgaagttc gtgcgtatcg acgaaccgga
     2881 cctgtcgtta ctgaagaagg gtgactatct tggcatcgcg ttgatgtcgg ggttcctcgg
     2941 ctgccttgaa tatgtgttgg aggagggacc gcgcaagaac tggttcggcg acgatgtgat
     3001 catcacctgc acgtggattt ccgctatctg tggcttcctg tttctcgtgc acgcgttcac
     3061 tgcgaaagat cctgttgtcg atctgcgcgc gctcgcggtc aggaactttg cgatcggttg
     3121 tttgctgtcg ttcgtgacgg gtatcggcat cttcgtcgcc gtgtttctca cgccgctgtt
     3181 tttgagccgc gtgcgtgaac tggattcgct gcagatcggg attgcgctgt tgtcggtggg
     3241 cgtattccag cttcttgcgc tgggcgccta tgcattcgcc tcacgtctcg tcgacatgcg
     3301 ctggtgtctg ctctttggcc tcgtgtgttt cggcctgggc tgctatttct acgtgccgct
     3361 cacgaacgat tggggctggc agcaactgtt gctgccgcag gcgctgcgag ggattggcca
     3421 gcagttcgcg gtgccgccta tagtaaccat ggcgctgggc tcgttgccgc aatcgcgcct
     3481 gaagtcggcc agcggcctgt tcaacctgat gcgtaacctc ggtggcgcaa tcggcattgc
     3541 ggtcagcgca accatgctca acgaccggct gaattttcac tatctgcgca tcagcgaaag
     3601 cgtcacgacc ggcagtccag agacggaagc gttcttgtcg aggcaattcg cgcactgggg
     3661 atcggtggcc ggagatgcgc tgaacgcgac caacgcgggc ctcgccaacc tgcatgcgat
     3721 ggtgtttcgc gaagcgctgg tgatgacctt ctccgacacc ttctatgtac tcgcgatgtg
     3781 ctttgccgtc ggcatagtca gcgtgctttt cgtgcggcct atcggcgctg cgccgccttc
     3841 tcctgacgct cattgagatc gaccatgtat aagattttat tggcattggc agcaagcatg
     3901 acgctcgcgg cgtgcgcggt gcaacccgcg agccatgcgg atttaccatc gaccgtacag
     3961 tcgatagcgc ccgcaaactg gagcgtcgac gccccgcagg acaacgtgga tgccgccgcg
     4021 tggtgggagc agttcggcga cccggtgctg catgaactcg tgagctcggt gctgagccag
     4081 aacctcgacg tgcaagctgc actggagcgc gtaaagcagg cgcaggccgt gaccacgcaa
     4141 cgtcgcgcgg cgcttctgcc ggaactcgat gcgagcgcca ccgcctccga tgcacgccag
     4201 aacacgccgc ccccgctggg ctatgttcgt caggcaggtt ttggcttgac gcttggctgg
     4261 acgcctgacg tattcggcgg cgagcgcctt gaactgttgg cggcacaggc gcagttgaca
     4321 ggccgtcaac atgatgtcga tgcgctgcgt cttgcgctcg cggccaatac ggcatcggcg
     4381 tatgtggatc tgcgctgggc gcaaagtgaa cagaagatct tgcgggacaa tatcgcgatc
     4441 cgggaacgtg cactgcgcct gacgcaggaa cgcatgcaat acgggctgtc gccgcgcctt
     4501 gacgtcactc gcgcacagaa ccagttgagc gaattgcaag cgcgtttgcc gcgtgccgac
     4561 gccaccgttc aacatcagct aagcctgatc gcggtgtatt cggggcgcac gcctgaatcc
     4621 gtcgataccc tcgtgctcgc cacgcccggg actattccga cgccgtcaaa cgccgtgccg
     4681 cagatgctgc cttcggaggc gttgctgcgc cgtcccgatg tccgcagcgc ctacgccgag
     4741 ctcacgcaac gcgcagcgga agtgggcgtc gcgcaagcgg accgctatcc gaagttctcg
     4801 ctgcaattga ccgacggttt gctggcgtcg tcgtatcttg gcttgcctac gcttaccgac
     4861 aacctcttca gcgctgcgct caatgcgacc agtcctatct tcaacgccgg gcgcattacg
     4921 gcgggcatcg agcagagcca aagccgtatg cgcgagtccg atctgcgctt gcgccagacc
     4981 atgctgcagg cgttaaaaga tgtggaggac acgcgtaccg atcttgtcag cacgtcggag
     5041 caggccggcc gcctgaagga cgcgttgcaa tcctctgatc agtcgttgcg cctctctacg
     5101 caactttata agggcggtgc gagcggtttc ctcgatgtac tcgcggcgca ggaagcgtat
     5161 ctgcaggact cggatgcgtt gaaccaggcg agacgtgaac acgcgctggc agcggtcgcg
     5221 ctgtaccgtt cgcttggtgg aggctggagc gaagcaaagg acgagtaatg cttggtgcgg
     5281 gcctcgggta acatcgggca tctttttgtt caagttgttt aaccacccga tggcactcaa
     5341 aaacctgttg agacggcttg acctgaccac gctgcaactc ttcctcgcgg tctacgagga
     5401 gggcacgctc acgcgggcgg ccgagcgcga ggcgatcgct gtatcggctg cgagcaagcg
     5461 cttgctcgaa ctcgaacagg ccgtgggcgc aacgctgttc gtgcgccggg cacgcggcat
     5521 ggagctcacg cccgcaggcg aaacccttct gcatcatgcg cgccgcgtgt tgcgcgatgt
     5581 cgagaacatc gggatcgaat tggccgagca cgcgaccggc gtgcgcggtt atgtccgcat
     5641 gatggcgaac ctgtcggcca tcgtggaatt ccttcccgaa gacctgcgcg cattcttctc
     5701 gatgaacgag cgcatcaagc tcgacctgga agagcgcccg agcagcggcg tggtgcaagg
     5761 catagcagac agcctcgccg acgtcggcat ctgctcaggc gaagccgata cgcgcggcct
     5821 cgatgtctcg cactaccggc atgactcgct ggtcgtggtg atgcgtgagg atcatgcgct
     5881 cgcggcccgc gagcaggtca cgttcgttga aacgctggat agcgactatg tcggtctgca
     5941 tgccgccagc tcgatcaacc tgcgtacgca aacggccgcg cggcaggcgg gtaaaccatt
     6001 gcggctgcgc attcaggtgc caggtttcga tggcgtctgc cgcatggtgc agacgggtct
     6061 gggcatcagc gtgctgccgc tgaaggtctt caatacagta ggccgtccac tcgggctgac
     6121 agccgttacg ctcgacgaaa cctggagcca gcgcgacctg atcgtcgtgg tacgcgatgt
     6181 tgcggggctc ccgccgctta gccggatgct gttcgatcat ctgcgtgccg tggagcacca
     6241 ggaacagaat aaagcctgag cgttcgccgt tcgcgaacgg tcattggcga aatgcggttg
     6301 gacgcggtgc gttgcccgct cctaagctgt cttccagatt agaattttcg ggagacaagc
     6361 atcatggctg gaccgctcgc aggcattcgc gtcatagaaa tcggcaccct gatcgcggcg
     6421 ccgttcgctg cccgcatgct cggggagttc ggcgccgacg tcatcaagat tgaggcgccg
     6481 gataacggcg acccgcttcg caaatggcga aaactgcacg agggcacgtc gctctggtgg
     6541 tatttgcagt cgcgcaacaa aaagtccgtg tgcgtcaacc tgaagtcccc ggacggtgtc
     6601 gatgtcgtca agcgtctcgc cgcggacgcc gacgtcgtca ttgaaaacct ccgtcccggt
     6661 gcgctcgaaa aactcggcct tggctgggac gtactgcacg caatcaatcc caaactcacg
     6721 atggtccgca tctccggata tgggcagtcg ggaccgtatc gcgatcgtcc gggcttcggc
     6781 gcgatcggcg aggccatggg tggcattcgc tatacaaccg gcgaagtcgc tggcgcaccg
     6841 gcacgcgtgg gcgtgtctct cggcgactcg ctggcttcgc tgcacggggt gatcggcgcg
     6901 ttgatgtcga tcctgcgcgt gaagaccggg cagggcgagg ggcaagtcgt ggatgtatcg
     6961 ctcgttgaaa gcgtcttcaa cctgatggaa agcctgatcc ccgaatacga tctgctcggg
     7021 cacgtgcgcg agcgcagcgg cggtgcgttg cctggcatcg cgccgtcgaa tac
//
LOCUS       DATASH010000485         3014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2647608,
            whole genome shotgun sequence.
ACCESSION   DATASH010000485 DATASH010000000
VERSION     DATASH010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2647608"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      200..336
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            370..1791
                     /gene="ahcY"
                     /locus_tag="VHV99_13400"
     CDS             370..1791
                     /gene="ahcY"
                     /locus_tag="VHV99_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX3636462.1"
                     /translation="MNAAVLDSNSKQESVVADMSLAAWGRKELNIAETEMPGLMQTRE
                     EYKTSQPLKGARIAGSLHMTIQTGVLIETLTALGADVRWASCNIFSTQDHAAAAIAEG
                     GVPVFAFKGESLDEYWEFSHRIFEWPNGEFANMILDDGGDATLLLILGSKAEKDRSVI
                     SKPENEEEVALYKSIASHLDADPVWYSTRLAQIKGVTEETTTGVHRLYQMEKEGRLPF
                     PAINVNDSVTKSKFDNLYGCRESLVDGIKRATDVMIAGKIAVVAGYGDVGKGCAQSLR
                     GLGATVWVTEIDPICALQAAMEGYRVVTMEYAADKADIFVTATGNFHVIGHDHMAAMR
                     NNAIVCNIGHFDSEIDVASTRKYTWDNIKPQVDHIIFPDGKRIILLAEGRLVNLGCAT
                     GHPSFVMSASFTNQTIAQIELFVHGDKYENKVYVLPKHLDEKVARLHLGRIGAELTVL
                     SDYQANYISVDKNGPFKPNHYRY"
     gene            1963..2316
                     /locus_tag="VHV99_13405"
     CDS             1963..2316
                     /locus_tag="VHV99_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018432546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX3636463.1"
                     /translation="MTVLLTWLINALALLIITYLVPSIHIRSFGTALIVAVVLGLINT
                     ILRPVLILLTLPVTILTLGLFILVVNALCFWLCASLLKGFEVSGFWSAFFGSILYSIV
                     SWLLSALIFGNRSLG"
     gene            2338..>3014
                     /locus_tag="VHV99_13410"
     CDS             2338..>3014
                     /locus_tag="VHV99_13410"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006052550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEX3636464.1"
                     /translation="MNPIELSFEFFPPKTQEGVDKLRATRAQLASLKPKFVSVTFGAG
                     GSTQQGTLDTVVDMAKEGLEAAPHVSCIGSSKESLRAILNEYRAHGIRHIVALRGDLP
                     SGMGEVGELRYASELVSFIRAEFGDWFWIEVAGYPEYHPQSRSPRHDLENFAHKVKAG
                     ANSAITQYFFNADAYFRFVDDARKLGVEVPIVPGIMPITNFSQLMRFSEMCGAEVPRW
                     VARRLES"
BASE COUNT          613 a          923 c          869 g          609 t
ORIGIN      
        1 aaagccccaa agccccaaag ccccaaagcc ccaaagcccc aaagccccaa agccctaaag
       61 ccctaaagcg ttctggcacc aagccgaacc gcgccagtcc accatcgcat tcggctttat
      121 ataagggcgc cgccaaaagc gccccccttc aacccgttcc acgccccgaa gccatctcga
      181 tttgctacaa tcgccgttcg tcttcgagga gcgttgcgac gggtaccgcg aatcagcata
      241 ttcgccggcc gcccgccagg ctcgaaggct tcaatcggag ggatcggatg cgcatagcgc
      301 tttcccatcc accgcatcga accgcgctca cgtcaaattt ctagtcaatt ttttagaaag
      361 gagggcgtga tgaacgccgc tgttctcgat tcaaattcca aacaggaatc cgttgttgcc
      421 gatatgtcgc ttgccgcctg gggccgcaag gaactgaata tcgctgaaac cgaaatgccc
      481 ggcctgatgc aaacgcgcga ggaatacaaa acctcgcagc cgttgaaggg cgcgcgtatc
      541 gcgggttcgt tgcatatgac gatccaaacc ggcgtgctga tcgaaacctt gacggcactc
      601 ggcgcggacg tgcgctgggc ctcgtgcaat attttctcga cgcaggatca tgcagccgcc
      661 gcgattgcgg aaggcggcgt gccggtgttc gcattcaagg gcgaatcgct cgacgaatac
      721 tgggaattct cgcaccgcat tttcgaatgg ccgaacggcg aattcgccaa catgatcctc
      781 gacgacggcg gcgacgccac gttgctgctg atcctcggct cgaaggctga gaaagaccgc
      841 tcggtcattt cgaagccgga aaacgaagaa gaagtcgcgc tgtacaagtc gatcgccagc
      901 cacctcgacg ccgatccggt gtggtactcc acgcgtctcg cgcagatcaa gggcgtgacc
      961 gaagaaacca cgaccggcgt gcatcgtctg taccagatgg aaaaggaagg ccgcctgccg
     1021 tttccggcca tcaacgtcaa cgactcggtc accaagtcga agttcgacaa cctgtatggt
     1081 tgccgcgagt cgctggtcga cggtatcaag cgcgcaaccg acgtgatgat cgccggcaag
     1141 attgcggtcg tggcgggtta cggcgacgtg ggcaagggct gcgcgcaatc gctgcgcggt
     1201 ctgggcgcca cggtgtgggt cacggaaatc gatccgatct gcgcactgca ggcagccatg
     1261 gaaggctacc gcgtcgtgac gatggagtac gccgccgaca aggccgacat tttcgtcacg
     1321 gcaaccggca acttccatgt gatcggccac gatcacatgg ctgccatgcg caacaacgcg
     1381 attgtctgca atatcggtca cttcgattcg gaaatcgacg ttgcttccac tcgcaagtac
     1441 acgtgggaca acatcaagcc gcaagtcgat cacatcattt tcccggacgg caagcgcatc
     1501 atcctgctgg ctgaagggcg tctggtgaat ctgggttgcg cgacgggcca cccgtcgttc
     1561 gtgatgtcgg catcgtttac gaaccagacg atcgcgcaga tcgaactgtt cgttcacggc
     1621 gacaagtacg aaaacaaggt ctacgtgttg ccgaagcacc ttgatgaaaa ggtcgcgcgc
     1681 ctgcatctgg gccgtatcgg cgcggagctg acggtgttgt cggactatca ggcgaattac
     1741 atcagcgtcg acaagaacgg tccgttcaag ccgaaccact accgttacta agtggccgct
     1801 gttacgtcgc cgcagttaaa tagccgctgt tacgtagcat caggcacgcg ggcggtttaa
     1861 aagcatgaca gtttgacgac acggcggcgc gcaatgcgcc gctttgtttg ccatgcccgc
     1921 ctctttccgt tcaaccggac atcctgccaa aaggagctct acatgaccgt gctgctgacc
     1981 tggctgatca acgcgcttgc gctcctgatc atcacctacc tcgtcccgtc gattcatatc
     2041 cgcagtttcg gtaccgcact gatcgtcgcg gtcgtgctcg gattgatcaa tacgatcctg
     2101 cgaccggtgc tgatcctgtt gacgctgccc gtgacgatcc tcacactcgg actcttcatt
     2161 ctggtggtca atgcgctgtg cttctggctg tgcgcgtcgc tgctcaaggg cttcgaggtg
     2221 tcgggtttct ggtcggcgtt cttcggctcg attctgtaca gcatcgtttc gtggctgctg
     2281 tccgcgctta ttttcggcaa ccgcagtctt ggttgaccca cggttgacta ctgaatcatg
     2341 aacccgattg aactttcatt cgaattcttc ccgcctaaaa cgcaggaagg cgtcgacaag
     2401 ctgcgcgcca cccgcgcaca gctcgcctcg ctcaagccca agttcgtctc cgtgacgttt
     2461 ggcgccggcg gctcgacgca gcagggcacg ctcgataccg tcgtcgatat ggcgaaggaa
     2521 gggctcgaag cggcgccgca cgtgtcgtgc atcggctcgt cgaaggagag cctgcgcgcg
     2581 atcctgaacg agtaccgcgc gcatggcatc cgccatatcg tcgcgctgcg cggcgatctg
     2641 ccgtccggca tgggcgaagt cggcgagctg cgctacgcgt cggaactggt gagctttatc
     2701 cgcgctgagt tcggcgactg gttctggatc gaggtggccg gctatccgga gtaccacccg
     2761 cagtcgcgct cgccgcgtca cgatctggaa aacttcgccc ataaggtgaa ggccggcgcc
     2821 aattccgcaa tcacgcagta tttcttcaac gccgacgcgt acttccgttt cgtcgacgac
     2881 gcgaggaagc tcggcgtcga ggtgccgatc gtgccgggca tcatgccgat cacgaacttc
     2941 tcccaactga tgcgtttctc ggagatgtgc ggtgcggaag tgcctcgctg ggtcgcgcgc
     3001 cgtctggaaa gctt
//
LOCUS       DATASH010000486         3514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1461049,
            whole genome shotgun sequence.
ACCESSION   DATASH010000486 DATASH010000000
VERSION     DATASH010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1461049"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..138
                     /locus_tag="VHV99_13415"
     CDS             <1..138
                     /locus_tag="VHV99_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEX3636465.1"
                     /translation="ADQPHSLRNPGDTEARAFRVTLKAETPPRWDVPAPHDLTRQAAP
                     V"
     regulatory      260..488
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            635..1354
                     /gene="ribB"
                     /locus_tag="VHV99_13420"
     CDS             635..1354
                     /gene="ribB"
                     /locus_tag="VHV99_13420"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEX3636466.1"
                     /translation="MSFATFPAPSVIADSASLDLPLLATEPVPPRIAAALQAMRDGRA
                     VVLQDDHDRENEADLIVSAERLSVETMALLIRECSGIVCLCLPDDKIRALELPPMAVN
                     NESRHGTAFTVSIEARDGVTTGVSALDRVTTIRAAISDTAKPADIVRPGHVFPLRAQP
                     GGVLARRGHTEGTVDLAILAGLKPAGVLCELMNADGTMTRGADVERFAAQHNLPMLTI
                     AELVEFRQALATARECVADEA"
     gene            complement(1479..1859)
                     /locus_tag="VHV99_13425"
     CDS             complement(1479..1859)
                     /locus_tag="VHV99_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636467.1"
                     /translation="MDTYSFHDWFVTGVSVDIAKKRVVLTLQSDNQRERATLTFSGAP
                     RCVVEGFAAQNIIYELKSIEDFQAPSYKSALKALEQAHPWGNDWPLRKIVTISSSLGA
                     EIIVEYDELTIEYSETQPSGLAPD"
     gene            complement(1940..2356)
                     /locus_tag="VHV99_13430"
     CDS             complement(1940..2356)
                     /locus_tag="VHV99_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012493440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4279 domain-containing protein"
                     /protein_id="HEX3636468.1"
                     /translation="MKSHQLAHASFTISGHQVVPEFWTDYFGVVPDIAIKEGDAINDP
                     TGQGRILTRRTGVWGMRSQTAVRSDSLTPHLRYLLDQLRLPRDDLRRLIKAIDGQMRF
                     FCYWDNRTGDRVPDVPEDIRAMMEFLGGVIEIDEYR"
     gene            complement(2410..3096)
                     /locus_tag="VHV99_13435"
     CDS             complement(2410..3096)
                     /locus_tag="VHV99_13435"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX3636469.1"
                     /translation="MIDHLICDCDGVLVDSEIIADRVMLETLSATFPGLDFEAVVKTA
                     FGQQTSRFLEGIEKSFDITLPAGFFNTIEHNVELALAASLSPINGVRDALQRVTLPAA
                     VVSNSRLARVNASVRRAGLQQIFGERIFSAEQVARPKPYPDVYLFVAKTLGVEPARCI
                     VVEDSVAGLNAARAAGMKTIAFVGASHIPDGYADALRKMGMTRIMKHMDELPGLVEAG
                     VRGEFGDVQS"
     gene            complement(3327..>3514)
                     /locus_tag="VHV99_13440"
     CDS             complement(3327..>3514)
                     /locus_tag="VHV99_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEX3636470.1"
                     /translation="LGGPTGYAMSPARDLSPRLMHALLPIPGKRDSDWRYSWIPVCGP
                     LLGGAVAAALYLYLHAH"
BASE COUNT          688 a         1055 c         1059 g          712 t
ORIGIN      
        1 gccgaccagc cgcacagcct gcgcaatccc ggcgacacgg aggcgcgcgc gtttcgtgtg
       61 acgctgaagg cggaaacgcc gccgcgctgg gacgtgcccg caccgcacga tttgacgcgc
      121 caggccgcgc cggtttgacg gatgaggttt atcaggcgga tgtgctgtga agcagagatg
      181 tccgcgtgaa cagtaggggt tgcgctgcag cgtccgcgcg actgttgtat gcttcgccag
      241 ccggtcgatc cggcaatcag tgcgtcttca gggcggggtg aaattcccca ccggcggtat
      301 gccggcaacg cgaaagcgtg agccggtgag cccgcgagcg cctgtgtcgt caaggtgttt
      361 ggatctgtgc aactacgttg catgggacca gagtaagcgc tgaagcgcta actctggtcg
      421 acacggggtc agcagatctg gtgagaagcc agagccgacg gttagagtcc ggatggaaga
      481 agatgtgcag atggtcatgt tcgtttccgt ggcgccgcca gaggtaagag cggtgcgcga
      541 gcgtcgcctt gcgcggcggt ccacggcgcg tctttgtctg tttgcaatgc cctgaaacgt
      601 ttttcgccca acttttgcga tgagcgtttc aactatgtct tttgctactt ttcctgctcc
      661 gtcggtgatt gcggatagcg catcactcga tctcccgctg ctcgccaccg aacccgttcc
      721 gccgcgtatc gccgccgcct tgcaagccat gcgcgatggc cgtgccgtcg tcctgcaaga
      781 cgatcacgac cgcgagaacg aagccgatct gatcgtttcc gccgagcgtc tttccgtcga
      841 aaccatggcc ttgctgatcc gcgaatgcag cggcatcgtc tgcctgtgcc tgcccgacga
      901 caaaatccgc gcgcttgaat tgccgccgat ggccgtcaac aacgaaagcc gtcatggcac
      961 cgcgttcacg gtctcgatcg aggcacgcga tggcgtgact accggtgtgt cggcactcga
     1021 tcgcgtgacg acgattcgcg ctgccatcag cgatacggcg aagcctgccg atatcgtgcg
     1081 cccgggccat gtgttcccgt tgcgcgcgca gcccggcggt gtgctcgcgc gccgcggcca
     1141 cactgaaggc acggtcgacc tggcgattct cgcgggtttg aagccggccg gcgtgctgtg
     1201 cgaactgatg aacgcggatg gcacgatgac gcgcggcgcg gacgtggagc gcttcgccgc
     1261 gcaacacaat ctgccgatgc tgacgatcgc cgagctggtg gaattccgtc aggcgttggc
     1321 gacggcgcgc gaatgtgtgg ccgacgaggc ttgatgcgga ccgacgcagc ttgttggatg
     1381 gaagaggccg acggcatgtc ggtctctttt atggcgtaag cacgcgacac gatgccgtgt
     1441 gatgccagaa tgctttttcg acgtccgggc gcgtgagttc aatcaggggc caacccggat
     1501 ggttgagtct cgctatattc gatagttagc tcgtcatact caacgataat ctctgcgccg
     1561 agagaactgc ttatcgtgac aattttccgc aacggccagt catttcccca agggtgcgcc
     1621 tgctccaggg ctttcaatgc cgatttgtaa gaaggtgcct ggaaatcttc tatcgatttc
     1681 agttcgtaga tgatgttttg agcggcaaaa ccttcaacta cacagcgcgg agcgcccgaa
     1741 aaagtaagtg tcgcacgctc cctttggttg tcgctttgca gcgtgagcac tacgcgtttt
     1801 ttggcaatgt ctacggacac gccggtcacg aaccaatcgt ggaatgaata cgtatccaac
     1861 tgactttcct caacgagccc acgcgctgaa atcgctccag gcccgacgtc gttgccttgt
     1921 tgcttgagcg agcaatagac tatcgatatt cgtcaatctc tatcacgcct cccagaaact
     1981 ccatcattgc gcggatgtcc tcgggtacat cgggcacgcg gtcgccagtc ctgttgtccc
     2041 aatagcagaa gaatcgcatc tgcccgtcaa ttgcctttat aaggcggcgc aagtcgtctc
     2101 gaggcagtcg aagctgatct aacaggtaac gaagatgcgg agtcagtgag tcgcttctta
     2161 ctgcggtttg actgcgcata ccccaaacac ccgtgcgtct ggtcaaaatc cgaccttggc
     2221 cggtgggatc gttaatcgcg tccccctctt taatcgcgat atccgggacg acgccgaaat
     2281 agtcggtcca aaactcgggc acgacctggt ggcccgatat tgtgaatgat gcgtgcgcaa
     2341 gctgatggga tttcatatgc taacgcctgc cgcgatgcaa aacgaagacc gccttgttat
     2401 tgcgaacgac tacgactgca cgtcgccaaa ctcaccacgc acaccagcct cgaccagccc
     2461 cggcagttca tccatatgct tcatgatgcg tgtcatcccc atcttgcgca gtgcatccgc
     2521 ataaccatcg gggatatggc tcgcacccac gaaggcgatc gtcttcatgc ccgccgcccg
     2581 cgccgcattc aaaccggcca cgctgtcttc cacgacgatg catcgcgccg gctcgacacc
     2641 cagcgtcttc gcaacgaaca gatacacgtc cggatagggc ttcggtcgcg cgacctgttc
     2701 ggcgctgaaa atccgttcgc cgaaaatctg ctgcaagccc gcgcgccgca ccgacgcatt
     2761 cacgcgcgct agccggctgt tcgacacgac cgcggccggc agtgtcacgc gttgcaatgc
     2821 gtcacgcacg ccgttgatcg ggctgagcga tgctgcaagc gcgagctcga cgttgtgttc
     2881 gatcgtattg aagaaaccgg caggcagcgt gatatcgaac gacttctcga taccttcgag
     2941 aaagcgcgag gtctgctggc cgaacgccgt tttgacgacg gcttcgaagt cgaggccggg
     3001 aaaggtggcg gacagcgttt cgagcatcac gcggtcagcg atgatttcgc tgtcgacgag
     3061 cacgccgtcg cagtcacaga tgaggtggtc gatcatgctt taaaacggga agcgagaggc
     3121 acgcgagcag gacagttgca agctcatgcg cgaagattga aagcgtcatg atacgtgctt
     3181 tggcgccgca tgcgcggcgc caaagctttc cgtcggcgct aattttcgcc tcgagcgtgc
     3241 cgttagtgcg cgttgtgcgc gccatggatg cgcgttaggt gccccctgaa tggtccgcct
     3301 aagtgcgtca taaagtcacc gcggaatcag tgcgcgtgca ggtacagata cagcgccgct
     3361 gccaccgcgc cgcccagcag tgggccgcac actggaatcc acgaatagcg ccagtcactg
     3421 tcgcgcttgc ccggaatcgg cagcaacgcg tgcatcagac gcggcgagag gtcgcgcgcc
     3481 gggctcatcg cataaccggt cgggccgcca agcg
//
LOCUS       DATASH010000487         5250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_868204,
            whole genome shotgun sequence.
ACCESSION   DATASH010000487 DATASH010000000
VERSION     DATASH010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5250
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868204"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="VHV99_13445"
     CDS             <1..521
                     /locus_tag="VHV99_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX3636471.1"
                     /translation="LILEKRLERLFHDLRIVAIALIVNGVLLWFGDRLQRSRVHRAPE
                     KLTFRQAFFVGLAQIGALIPGFSRSGLTMIAGNAAGLTTEKAAEFSFLLGTPIILAAG
                     VLELPKLFHAPGQLADALLGGVLTAIAAYLSVRFLMRYFEGRGRLASFGVYCVIAGIV
                     FLGWFMLHPQPV"
     gene            835..908
                     /locus_tag="VHV99_13450"
     tRNA            835..908
                     /locus_tag="VHV99_13450"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:869..871,aa:Arg,seq:tct)
     gene            complement(1003..1386)
                     /locus_tag="VHV99_13455"
     CDS             complement(1003..1386)
                     /locus_tag="VHV99_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEX3636472.1"
                     /translation="MTEKTIKVPGPDHPITVEPDTSHVVVTVAGRVIADTRKALVLRE
                     ASYPPVYYIPRNDVEMTLLERTDHATYCPYKGDCAYYSIPLGGERTVNAVWTYESPYA
                     AVKEIAQHLAFYPNRVDSIEVQPAG"
     gene            complement(1597..1836)
                     /locus_tag="VHV99_13460"
     CDS             complement(1597..1836)
                     /locus_tag="VHV99_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636473.1"
                     /translation="MDISFALESFAVALALFCVALVPFAIGRDPGLARRIVQMDNLPR
                     ARWPNLLTRVGIACVLMSFALLLAGCYYILAGAGN"
     gene            2120..2509
                     /locus_tag="VHV99_13465"
     CDS             2120..2509
                     /locus_tag="VHV99_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RT0821/Lpp0805 family surface protein"
                     /protein_id="HEX3636474.1"
                     /translation="MSTRTRALFHLTVGGLLLGGAMGAQAANLGFLNDTPISYMKQRD
                     IDSVKKAVVAALNDKQDGESTSWVNEGTGNSVRIDATITVASTAKDGERTCRDVGVVL
                     NAKGQSMNLRPQFCKQASGNWQLQKKR"
     gene            2592..3041
                     /locus_tag="VHV99_13470"
     CDS             2592..3041
                     /locus_tag="VHV99_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX3636475.1"
                     /translation="MIARVISCGIVLLDPDGRLLLAHATETSHWDVPKGHGEEGEAPH
                     VTALREMIEETGIAIEAGRLKDLGLFVYRRDKDLHLFAARATADELDLSSCTCTSLFP
                     RRSDGRMIPEMDAYRWTAPDEVERYASRSLTRLFQTTLSLVELHRTL"
     gene            complement(3132..4025)
                     /locus_tag="VHV99_13475"
     CDS             complement(3132..4025)
                     /locus_tag="VHV99_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007744029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX3636476.1"
                     /translation="MRGALAMLVAFGTASLACPAHADELTGTLKKIHDDGVVVLGVRE
                     ASIPFSYFDGTRTVGYSQTIALQIVDEIKKTLGLPQLKVHEITVTSSNRTPMLLNNQI
                     DLECGSTTHTVERENVAAFSNSFFQYAVRMIARKSSGVTDFPDLAGKTVVTTAGTSDE
                     RLLRRLNTEKHLNMRITSARDHSDAFSALKDDRAVAFVMDEPILYGFKATDPHPEDFV
                     VTGTPLDYEAYACMFRKGDEPFRTLVNGVIARGQTSGDAERLYKQWFTQPIPPHGINL
                     NFPLSEQNRALFAHPNDRALD"
     gene            complement(4294..>5250)
                     /locus_tag="VHV99_13480"
     CDS             complement(4294..>5250)
                     /locus_tag="VHV99_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006764572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HEX3636477.1"
                     /translation="ILNELAEAGIFKRPLPQQIEFSRLNLTYAITSKRKLLQLVTEGH
                     VDGWDDPRMPTIVGLRRRGFTPESIQLFCERIGVTKVDSWIDMSVFEGALRDDLDAKA
                     PRTVAVLDPVKLIIDNFPEGVTEPCNAPVHPHHPEMGVREFPISRELLIERDDYNETP
                     PKGYFRLFPGNKVRLRYGYVIECIGADKDENGNIVAVHCNYFPDSKSGTEGANAYKVK
                     GNIHWVSAAGACPAEVRIYDRLFKEPQPDAGGRDFLDALNPDSKRVVNAYLEPGARDA
                     LPEQRYQFERHGYFVADRVDSKPGKPVFNRIVSLRDSWGKPA"
BASE COUNT          916 a         1584 c         1699 g         1051 t
ORIGIN      
        1 gactaatcct cgagaagcgc ctcgagcggc tcttccacga tctgcgcatc gtcgccatcg
       61 cgctgatcgt caacggtgtg ctgctgtggt tcggcgatcg tctgcaacgt tcgcgcgtgc
      121 atcgggcgcc ggagaagctg acgttccgcc aggcgttttt tgtcggtctt gcgcaaatcg
      181 gcgcattgat tccgggcttc tcgcgtagcg gtctgacgat gatcgccggc aatgcggctg
      241 gcctgacgac cgagaaggcc gccgagttct cgttcctgct gggcacgccg atcatcctcg
      301 cggccggcgt gctcgaattg ccgaagttgt tccatgcacc gggccaactc gccgatgcat
      361 tgctcggcgg cgtgctgacc gcaatcgccg cgtatctgag cgtgcgtttt ctgatgcgct
      421 atttcgaagg ccgcggacgt ctggcttcgt tcggcgtgta ctgcgtgatc gcggggatcg
      481 tgttcctcgg ctggttcatg ctgcacccgc agccggtctg attgcgttcg atatgtccgc
      541 tgctgcgcgc cttgcgctgg cgtgcggttc gcgggcgaga ggcgatcggt tataatccgg
      601 gcccgatgca acctgccggg cccgttttgt ttggtggcga ggcgtgggtg gctgccggtc
      661 agcgttgccg cgcggcggat tggatttcca agccttggct cggtcagtta gaatttcggt
      721 atcccacgaa ccctgtggca gtgatagtgc acaggttcca atgccctgta cgggttctca
      781 ttgcgggcga tgcatggtgt acacggttgt cgtgctgcat cgcctcacgt caatacggca
      841 gtagctcagc tggatagagc atcggccttc taagccgagg gtggggggtt cgagtccctc
      901 ctgccgtact tcaaatgtct ggcgccgcgc atcgaagcct caccgctgcg atgcgcgctt
      961 cgccatgcgt cttgccgtgc attccggcgc gcattccgcc gtttaccctg ccggctgcac
     1021 ttcgatggaa tcgacccggt tcggatagaa cgccagatgc tgagcgatct ctttcacggc
     1081 cgcgtagggc gactcgtagg tccacaccgc attcaccgtc cgctcgccgc caagcggaat
     1141 gctgtaatac gcgcaatcgc ccttgtatgg gcaataggtc gcgtggtcgg tgcgttccag
     1201 cagcgtcatc tcgacgtcgt tgcgcggaat gtaatacacc ggcggatacg acgcctcgcg
     1261 cagcaccagc gccttgcgtg tatccgcgat cacgcggccg gccaccgtga ctaccacatg
     1321 ggaggtgtcc ggctcgaccg tgatcggatg atccgggcct gggaccttga tggttttttc
     1381 agtcatgttg tcgaactcct gaatgagtgg gcgagcctgc aacacgtcgc gaagggtttc
     1441 gatgcgcaat cggggcggga atgatcgcgc tctgcgtcga agcatacgcc ggtcatgaaa
     1501 acgcgtcgtg tcgccgtcgg ggtcgtgctg ccttgcggct tgtagagcgg cacttgaaga
     1561 gcggcacttg atgcgacatg cgccgccgcg ggttggtcag ttgcccgcgc cggcaaggat
     1621 gtaatagcag ccggctagca gcaatgcgaa cgacatcagg acgcatgcaa tgccgacgcg
     1681 tgtcagcaga tttggccagc gtgcgcgtgg gaggttgtcc atttgcacga tgcgccgcgc
     1741 aagaccggga tcgcggccga tggcgaacgg caccagcgca acgcaaaaga gcgcgagcgc
     1801 gacggcgaag ctttctagag caaacgagat gtccatgagg cgacgttgga agctgggtag
     1861 gcgctgcagc gcaacggacc catcttaaga agacggttca taacaagata tgagacgcgt
     1921 cctaaaattc cgcaaagccc ctttcgtgcc tagtcgttcc ggcggggcgg ccgttggtgg
     1981 ctacagactg cggcaagcac tgcaaggcgc acatccagac ggccgcgccg agggcgacat
     2041 gctatgcttt tggggtccag atcatgagcc gcgtgagtgc gctcgagacc gccgaactac
     2101 tcttcgagga gaacccttca tgtcgacgcg aacccgtgcc ctgtttcacc tgaccgttgg
     2161 tggcctgctg cttggcggcg cgatgggcgc acaagcggcc aacctcggtt ttctgaacga
     2221 cacgccgatc agctacatga agcaacgcga catcgattcg gtcaagaaag ccgtcgtcgc
     2281 cgccctgaat gacaagcagg acggcgaaag caccagctgg gtcaatgaag gcaccggcaa
     2341 cagcgtcagg atcgatgcga cgatcaccgt cgccagtacc gcgaaggacg gcgagcgcac
     2401 ctgccgcgac gtcggtgtcg tcctgaatgc gaaagggcag tcgatgaacc tgcgcccgca
     2461 gttttgcaaa caggccagcg gtaactggca attgcagaaa aagcgctgag cgtgcaggcc
     2521 ggtatgacga cgcgcgtcgg cttgctgacg gcgcagcgcg aagccttgcg tctttgcggc
     2581 gcgttgcggc gatgatcgcg cgggttattt cgtgcggcat cgtactgctc gacccggacg
     2641 gtcggctgct gctcgcgcat gctaccgaga cgtctcactg ggacgttccg aaagggcacg
     2701 gcgaagaggg cgaggcgccg cacgtcacgg cgctgcgcga gatgatcgag gagaccggta
     2761 tcgcgatcga ggccgggcgt ctgaaggatc tcggcctgtt cgtctaccgg cgcgacaagg
     2821 atctgcatct gttcgcggcg cgcgctacgg ccgacgaact cgatttgagt tcgtgcacgt
     2881 gcacatcgct gtttccgcgc cgctctgacg gcagaatgat tcccgaaatg gacgcctatc
     2941 gttggaccgc gccggacgaa gtcgagcggt acgcgagccg cagtctgaca cggctttttc
     3001 agaccacgct ttcgctggtg gaactgcatc gaacgctata gcggcgggct tgtcgttgtg
     3061 gttgtggtgt ggttgtggcc gcaaccgcgc ggcgcaccgc cctcccgcct ccgcgtgggc
     3121 tggctgcgcg cttagtcaag cgcgcggtcg ttcggatgcg cgaacagcgc acggttttgc
     3181 tccgacagcg gaaaattcag attgatgcca tgcggcggga tcggctgcgt gaaccactgc
     3241 ttatatagac gctccgcgtc gccggaagtc tggccgcgcg cgatcacccc gttcacgagc
     3301 gtgcgaaacg gctcgtcgcc tttgcggaac atgcaggcgt aggcctcata gtcgagcggc
     3361 gtaccggtca ccacgaagtc ttcggggtgc gggtcggtcg ccttgaagcc gtacaggatc
     3421 ggctcgtcca tcacgaaggc caccgcgcga tcgtccttga gcgcgctgaa tgcatcagag
     3481 tgatcgcgcg cgctcgtgat gcgcatgttc aggtgcttct cggtgttcag gcggcgcagc
     3541 aggcgctcgt cggaggtgcc ggcggtggtc acgactgtct tgccggccag gtccggaaaa
     3601 tccgtgacac cgctactctt gcgcgcgatc atgcgcaccg catactggaa gaagctgttc
     3661 gagaaggcgg ccacgttttc gcgttccacc gtgtgcgtgg tcgagccgca ttccagatcg
     3721 atctggttgt tcagcaacat cggcgtacgg ttcgacgagg tcacggtgat ttcgtgcacc
     3781 ttcagttgcg ggaggccgag cgttttcttg atttcgtcga caatctgcag cgcgatggtc
     3841 tgcgagtagc ccaccgtgcg cgtgccgtcg aagtacgaaa agggaatcga cgcttcacgc
     3901 acgccgagca cgaccacgcc gtcgtcgtgg attttcttca gcgtgccggt gagttcatcc
     3961 gcatgagcgg gacaggcaag cgacgccgtg ccgaacgcga ccaacatcgc gagtgcgcca
     4021 cgcatgcttt gtgcgacgcg tgcggcgcga atcgtgcgcc gggcaggagc cgtacgcgcg
     4081 tgacgcacac cccgtgccgt gccccataca ctcgccccgc ggcgggctgc aactttcatc
     4141 aggcctcccg gactggcgcg tgcacgcatg ccgcgctgtc actatcgatg gaacatgatg
     4201 caacaaaacg gcgtgcatcc cgctcacgaa atgcacgccg tattcaactg caacctcaac
     4261 ttcagatgcg ctttgcgctg gaaccgcttc agcttacgcc ggcttgcccc aactgtcgcg
     4321 caaactgacg atgcgattga atacgggctt accgggtttc gaatcgaccc ggtcggcgac
     4381 gaaatagcca tggcgctcga actgatagcg ctgttccggc agcgcgtcgc gcgcgcccgg
     4441 ttccaggtag gcgttgacca cgcgcttcga atccggattc agcgcgtcga ggaagtcgcg
     4501 gccaccggcg tccggttgcg gttccttgaa cagacggtcg tagatgcgca cttcggccgg
     4561 gcacgcgcct gccgcgctga cccagtgaat gttgcccttg accttgtagg cgttcgcgcc
     4621 ttcggtgccc gatttgctgt ccgggaagta gttgcaatgg actgcgacga tgttgccgtt
     4681 ctcgtccttg tccgcgccga tgcactcgat cacatagccg tagcgcaggc gcaccttgtt
     4741 gcccgggaac aggcggaagt agcccttcgg cggcgtttcg ttgtagtcgt cgcgttcgat
     4801 caacagttcg cgcgaaattg gaaactcccg aacgcccatc tccggatgat gcggatggac
     4861 cggcgcattg catggctcgg tcacgccttc ggggaagttg tcgatgatca gcttgaccgg
     4921 atcgagcacg gcaacggtac gcggcgcctt cgcgtcgaga tcgtcgcgca gcgccccttc
     4981 gaacacgctc atgtcgatcc acgagtcgac cttggtgacg ccgatgcgct cgcagaacag
     5041 ctggatcgac tccggcgtga agccgcgccg gcgcaacccg acgatggtcg gcatgcgcgg
     5101 gtcgtcccag ccgtctacat ggccttcggt gacgagttgc agcagcttgc gcttgctggt
     5161 gatcgcatag gtcaggttca gacgcgaaaa ttcgatttgc tgcggcagcg gacgcttgaa
     5221 gataccggct tcggcgagtt cgttcaggat
//
LOCUS       DATASH010000488         8435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6999669,
            whole genome shotgun sequence.
ACCESSION   DATASH010000488 DATASH010000000
VERSION     DATASH010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 8435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8435
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6999669"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..500)
                     /gene="dut"
                     /locus_tag="VHV99_13485"
     CDS             complement(54..500)
                     /gene="dut"
                     /locus_tag="VHV99_13485"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015602380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEX3636478.1"
                     /translation="MKLDLKILDARMRDQLPAYATTGSAGLDLRACLDEPLTLKPGET
                     ALVPTGLAIHVGDPGYAALVLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISTWNRGE
                     TTFVLNPMERLAQLVIVPVVQAEFNIVDDFAESERGAGGFGSTGKH"
     gene            complement(515..1534)
                     /locus_tag="VHV99_13490"
     CDS             complement(515..1534)
                     /locus_tag="VHV99_13490"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX3636479.1"
                     /translation="MTLLSVLDQTPVIGGHSVADAIAATVELAQLADDLGYTRYWCAE
                     HHGLRGVSNPCPEVMLARLGSVTQRIRLGSGGIMLPYYSPFKVAEQFMMLEALFPNRI
                     DLGVGRAPGGDMHTAQAVAAGAYNRGDIFPQQVADLVGLMHGTLPEGHLAHGVLLQPQ
                     VDTRPQLWMLGSSEFGGLLAAQLGIRFAFAHFINAHFGHQVAHAYRERFKASQEAQQP
                     YLAAAVFVICADTEQEAADLEKAVDLRRVQMAYGLNEPIPTVEQGIAQEYGERERLVI
                     DREKPRSIIGTPETVTERLHALQEQFQADELIVLTVSGSYRARLRSYELLADAFQLGR
                     PVSTP"
     gene            complement(1610..2824)
                     /gene="coaBC"
                     /locus_tag="VHV99_13495"
     CDS             complement(1610..2824)
                     /gene="coaBC"
                     /locus_tag="VHV99_13495"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEX3636480.1"
                     /translation="MATAELAGKHLVLGMTGGIACYKIAELTRLLTKAGATVQVVMTE
                     AATQFITPVTMQALSGRPVYTSQWDGRVPNNMAHIDLSREADAIVIAPASTDFLAKLA
                     HGMADDLLSTLCVARDCPLLVVPAMNRQMWQNPATQRNVTLLRGDGVEVLGPDSGPQA
                     CGETGDGRMLEAAATFEAIASFFSPKILAGRRVLLTAGPTFEPLDPVRGITNRSSGKM
                     GFALARAAQQAGAEVHLVAGPVALETPWGVFRQDVQTAQQMHDAVMHAVADTDIFIGV
                     AAVADWRVDHASEHKIKKTADRALPTFTFVENPDILAAVAKLPNPPFAVGFAAESGDL
                     EVHGEEKRLRKNVPLLIGNLGPLTFGLDDNEVILFEAGGATKLPRADKQTLARALIVE
                     IARRLPDPSLIR"
     gene            complement(2935..3441)
                     /gene="lspA"
                     /locus_tag="VHV99_13500"
     CDS             complement(2935..3441)
                     /gene="lspA"
                     /locus_tag="VHV99_13500"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012399987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX3636481.1"
                     /translation="MAKTMSKTSAGNSSLAPWLGVALIVILFDQLTKIAVQKVFAYGV
                     PHEVAPFFNLILVYNRGAAFSFLAMAGGWQRWAFTALGVIAALVICYLLKRHGGQKMF
                     CTALSLILGGALGNVIDRLAYGHVIDFLDFHVRTWHWPAFNLADSAITIGAILLVLDE
                     LRRVRGSR"
     gene            complement(3441..6278)
                     /gene="ileS"
                     /locus_tag="VHV99_13505"
     CDS             complement(3441..6278)
                     /gene="ileS"
                     /locus_tag="VHV99_13505"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011352960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEX3636482.1"
                     /translation="MSNKKAESKPQSRYPVNLLDTPFPMRGDLPKREPQWVKEWQERK
                     VYETIRAASKGRKKFILHDGPPYANGDIHLGHAVNKILKDMIVKARNLAGFDAVYVPG
                     WDCHGMPIEIQIEKQFGKSLPAAEVMQKARAYATEQIERQKVGFRRLGVLGDWDNPYR
                     TMNFTNEAGEIRALAKILEKGYVFRGLKPVNWCFDCGSALAEAEVEYKDKTDPTIDVL
                     FSFAEPEKTAQAFGLGALPRNEGGIVIWTTTPWTIPANQALNLHPEIVYALVDTPRGL
                     LILAEERVEACLKQYGLEGTVIATTPGAKLVNLRFNHPLAAAHPSYKRTAPVYLGDYV
                     TTETGTGVVHSSPAYGVEDFVSCKAHGMSDSDIINPVMGDGRYIESLALFGGLSIWAA
                     NPQIVEALQNAGSLMRTEKYTHSYMHCWRHKTPIIYRATSQWFAGMDVKPNDTDKTLR
                     ETALEGIENTAFYPSWGKQRLFSMIANRPDWTLSRQRQWGVPMAFFVHKETGELHPRT
                     LALLEEVAQRVEKAGIEAWQTLDPRELLGDDANMYEKNRDTLDVWFDSGTTHWHVLRG
                     SHKDELQFPADLYLEGSDQHRGWFHSSLLTASMLDGRPPYNALLTHGFTVDGEGRKMS
                     KSLGNGIDPHEVANRLGAEIIRLWIASTDYSGELAISEEILKRVTEGYRRIRNTLRFL
                     LANLSDFDFAQHARPVEDWLEIDRYAVALSANLQNDILSHYDKYEFHPVVAKLQTFCS
                     EDLGGFYLDVLKDRLYTTAADSVARRSAQTVLYHIAHGLLRQMAPFLSFTAEEAWKIF
                     QPNSETIFTETYHAFPAVPDAGDLLDKWTLLRAARSDVTKALEEARVANLIGSSLQAE
                     VEIRASGARYEALASLGDDLKFVLITSAASVVKVDSEADEGVEVIASKYLKCERCWHY
                     RADVGANAEHPTLCGRCFSNLFGSGETRSAA"
     gene            complement(6448..7443)
                     /locus_tag="VHV99_13510"
     CDS             complement(6448..7443)
                     /locus_tag="VHV99_13510"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEX3636483.1"
                     /translation="MRVFRGLPNAESRAPCALTIGNFDGVHRGHQALLAHVRAAADAR
                     GLPVCVMTFEPHPREFFNPAGAPPRIAMLRDKLEALRTNGVDRVVVEHFNHTFASQSP
                     DAFVERIIVNGLHARWVMIGDDFRYGAKRAGDFASLKAAGQQHGFEVEQMATVADPSG
                     ARISSSGVRAALVAGDLDSARAALGRDYLISGHVVHGMKLGRDLGFPTLNLPIAHKRP
                     ALAGIFVVRVHGVADVPLPGVASLGLRPTVDDSGRVLLEVHLLDWHGDAYGKLVRVEF
                     LKKLRDEEKYVDLETLTAAISRDVVNARAWFAAVGAGTPGSRSTGFATSATDRIR"
     gene            7562..8215
                     /gene="purN"
                     /locus_tag="VHV99_13515"
     CDS             7562..8215
                     /gene="purN"
                     /locus_tag="VHV99_13515"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX3636484.1"
                     /translation="MKKLVILISGRGSNMEAIIRACADEGWAAQVAAVIANRPDAAGL
                     AFAASHGIATAVVDHRQFPDRDSFDAALAQTIDSFAPDLLVLAGFMRVLTAGFVDHYA
                     GRMLNVHPSLLPSFPGLKTHQQALDAGVRLHGASVHFVTSQLDHGPIVAQAAVPVEAG
                     DTPAMLAERVLATEHIIYPRAVRWFVEGRLALDGLRVTLTPPEPQWLFAGHTAGEGA"
     gene            8212..>8435
                     /locus_tag="VHV99_13520"
     CDS             8212..>8435
                     /locus_tag="VHV99_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004554551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX3636485.1"
                     /translation="MRLHGFLIGQTETLLAEVLKLNGPADATTSRFFRAHPKLGHGER
                     GVIAEAVFAVLRRRMEFAHLAEGGAGSPARR"
BASE COUNT         1467 a         2658 c         2746 g         1564 t
ORIGIN      
        1 gtggtcaatg ggcgcatttc ccacggggac tttataaacg ccaatgccgc atctcaatgc
       61 ttgcccgtgc tgccaaaacc gcccgcgcca cgctcgcttt ccgcaaaatc atccacgata
      121 ttgaactcgg cctggaccac cggcacgatc acgagttgcg caagccgctc catcggattc
      181 agcacgaagg tggtctcgcc gcggttccac gtcgagatca tcagctgacc ctggtaatcc
      241 gaatcgatca ggccgaccag gttgcccagc acgatgccgt gcttatgacc cagacccgag
      301 cgcggcagaa ccaacgccgc ataacccgga tcgccgacgt ggattgccag ccccgtcggc
      361 accaacgcgg tttcgccggg tttcagcgtc aacggttcgt cgaggcaggc acgcaggtcg
      421 aggcccgcgc tgcccgtggt tgcataggcc gggagttggt cgcgcatgcg cgcatcgaga
      481 atcttcaggt cgagtttcat gcaggttcga aggtttaagg ggtagagacc gggcgtccga
      541 gctggaacgc atcggcgaga agttcatagg agcgcagccg cgcgcggtag ctgcccgaga
      601 cggtgagtac gatcaattcg tcggcctgga actgctcctg caatgcgtgg agccgctcag
      661 tgaccgtttc cggcgtgccg ataatgctcc gcggtttctc gcggtcgatc acgaggcgct
      721 cgcgttcgcc gtattcctgc gcgatgcctt gttcaacggt cgggatcggt tcattcaggc
      781 cataagccat ttgcacgcga cgcaggtcga cggccttttc caggtcggct gcttcctgtt
      841 cggtgtccgc gcagatcacg aatacggctg cggccagata gggttgttgc gcttcctggc
      901 tggctttgaa gcgctcgcga taggcatgcg ccacctggtg cccgaaatgc gcgttgatga
      961 aatgcgcgaa cgcaaagcgg ataccgagct gtgccgccag caggccgccg aattcgctcg
     1021 aaccgagcat ccagagctgt gggcgcgtgt cgacttgcgg ttgcaggagc acaccgtgcg
     1081 cgagatgacc ttcgggcagc gtgccgtgca tcaggccgac tagatccgcg acctgctgcg
     1141 gaaaaatgtc gccgcgattg taggcgccgg ccgccaccgc ttgcgccgta tgcatatcgc
     1201 cgcccggcgc gcgtcccacg ccaaggtcaa tgcgattcgg aaacagcgcc tcgagcatca
     1261 tgaattgctc ggccaccttg aacgggctgt agtagggcag catgatgccg cccgagccga
     1321 ggcgaatgcg ttgggtcaca ctgccgaggc gcgccagcat cacttcgggg cacggattgg
     1381 acacgccgcg caagccgtgg tgttcagcgc accagtagcg cgtgtagccg agatcatcgg
     1441 caagttgcgc gagttcgacg gtggccgcga tcgcgtccgc caccgagtgc ccgccgatca
     1501 cgggcgtttg atcgagcacg gaaagtagcg tcatggcagt actgctccag atgattcggt
     1561 gcgccggagg cgctggtggc gaacgcggca ggcgccgcca cgcgcggttt tagcggatca
     1621 gacttgggtc gggcagacgt ctggcaatct caacgatcaa ggcgcgcgcg agcgtctgct
     1681 tgtcggcgcg cggcagcttc gtggcgcccc ctgcttcgaa caggatcacc tcgttgtcgt
     1741 cgagaccgaa agtcagcggg ccgagattgc cgatcagaag cggcacgttc ttgcgcaggc
     1801 gcttttcttc gccatgcact tcgaggtcgc cgctctccgc agcaaagccc acggcaaacg
     1861 gcggattcgg cagcttggcg accgctgcca gaatgtccgg attctcgacg aaggtaaagg
     1921 tgggcagtgc gcgatcggcg gtcttcttga tcttgtgctc gctggcgtga tcgacgcgcc
     1981 agtcggccac cgcggccaca ccgatgaaga tgtcggtgtc ggcgaccgca tgcatcaccg
     2041 cgtcgtgcat ctgctgcgcg gtttgcacgt cttggcggaa cacgccccac ggcgtttcca
     2101 gcgcgaccgg gcccgcgacc agatgcacct cggcgccggc ctgctgcgcg gcgcgcgcga
     2161 gcgcaaagcc catcttgccg ctcgaacggt tggtgatgcc gcgcaccgga tcgagtggtt
     2221 cgaaggtcgg ccccgcagtc agcaacacgc gacgccccgc caggatcttg ggcgagaaga
     2281 acgacgcgat cgcctcaaac gtggcggcgg cttccaacat gcgtccgtcg ccggtttcgc
     2341 cgcacgcttg cggacccgag tccgggccga gcacttcgac gccgtcgccg cgcagtagcg
     2401 tgacgttgcg ctgggtggcc gggttctgcc acatctgccg gttcatcgcc ggcacgacga
     2461 gcagcggaca atcacgcgcg acgcacaatg tcgagagcag atcgtcggcc atgccgtgcg
     2521 cgagtttggc gaggaagtcg gtcgaggcgg gcgcaatgac gatcgcgtcg gcttcacgcg
     2581 agaggtcgat gtgcgccatg ttgttcggca cgcgtccgtc ccattggctc gtgtagaccg
     2641 gacggccgga caacgcctgc atggtgacgg gggtgatgaa ctgcgtggcc gcttcggtca
     2701 tcacgacctg tacggtcgcg ccagccttgg tcagcaggcg cgtgagttcg gcgatcttgt
     2761 agcaggcaat gccgcccgtc atgccgagga cgaggtgttt tcctgcgagt tctgcggttg
     2821 ccaacgaaag cctccgacaa agcgtggtgg cgggcggcgt tactgtgcat ggccgcccgc
     2881 cgtcaaaacc ggaatccggg cggccgtgct tccggcgcct gtgcaggcac ctcgttagcg
     2941 cgagccgcgt acccggcgca actcatcgag cacgagcaga atcgcaccga tcgtgatcgc
     3001 gctgtcggcg agattgaacg ccggccagtg ccacgtgcgg acgtggaagt cgagaaagtc
     3061 gatcacatgg ccgtacgcga gccggtcgat cacgttgccg agggcgccgc cgagaatcag
     3121 cgaaagcgcc gtgcaaaaca ttttctgccc gccgtgacgc ttgagcaggt agcagatcac
     3181 cagtgccgct atcacgccga gcgcggtaaa cgcccagcgc tgccagccgc ccgccatcgc
     3241 gaggaagctg aaggcggcgc cacggttgta cacgaggatc aggttgaaaa acggcgcgac
     3301 ctcatgcgga acaccgtagg caaacacctt ctggaccgcg atcttggtga gctgatcgaa
     3361 caggatcacg atcagcgcga cgccaagcca cggcgccagc gaactgttgc cggcggacgt
     3421 tttcgacatg gtttttgcca ttatgccgcg ctcctcgttt caccgctgcc gaacaggttg
     3481 ctgaagcagc ggccgcacag cgtggggtgc tcggcgttgg caccgacgtc cgcgcggtag
     3541 tgccagcagc gttcgcactt caggtacttc gaggcgatca cttcgacgcc ttcgtccgct
     3601 tcgctatcga ccttgacgac gctcgccgcc gacgtgatca gcacgaactt cagatcgtcg
     3661 ccgaggctcg cgagcgcctc gtaacgcgcg ccgctcgcgc ggatttcgac ttccgcctgc
     3721 agcgacgagc cgatcaggtt cgcgacgcgt gcttcttcca gcgccttggt cacgtcgcta
     3781 cgcgcggcac gcagcagcgt ccatttgtcg agcagatcgc cggcgtccgg cacagccgga
     3841 aacgcatgat aggtctcggt gaagatcgtt tcgctgttcg gctggaagat cttccacgct
     3901 tcttctgcgg tgaacgacag gaacggcgcc atctgacgca gcagaccatg cgcgatgtga
     3961 tacagcacgg tctgagccga acggcgcgcg acggagtcgg ccgctgtggt gtacagacgg
     4021 tctttcagca cgtccagata gaaaccgccc aggtcttccg agcagaacgt ctgcagcttc
     4081 gcgaccaccg ggtggaactc gtacttgtcg tagtgcgaca ggatgtcgtt ttgcagattc
     4141 gccgacaacg ccaccgcata gcgatcgatc tcgagccagt cttcgaccgg acgcgcgtgt
     4201 tgcgcgaagt cgaaatccga caggttcgcg agcaggaagc gcagcgtgtt gcggatacgg
     4261 cgatagcctt cggtcacgcg cttcaggatt tcttccgaga tcgccagttc acccgagtaa
     4321 tcggtcgacg cgatccacaa ccggatgatt tccgcgccga ggcgattcgc cacttcatgc
     4381 ggatcgatac cgttgccgag cgacttgctc atcttgcggc cttcgccgtc gacggtgaag
     4441 ccatgggtga gcaacgcgtt gtagggcggg cggccgtcga gcatcgaggc ggtcagcagc
     4501 gacgagtgga accagccgcg gtgctggtcc gagccttcca gatacaggtc ggccgggaat
     4561 tgcagttcat ctttatgcga gccgcgcagc acgtgccagt gcgtcgtgcc tgaatcgaac
     4621 cagacgtcga gcgtgtcgcg gttcttctcg tacatgttgg cgtcgtcgcc gagcagttcg
     4681 cgcgggtcga gcgtttgcca cgcttcgatg ccggccttct cgacgcgttg cgcgacttct
     4741 tcgagcaacg ccagcgtgcg cggatgcagt tcgccggttt ccttgtgcac gaagaacgcc
     4801 atcggcacgc cccattgacg ctgacgcgac agcgtccagt ccgggcggtt ggcgatcatg
     4861 ctgaacaggc gctgcttgcc ccacgacgga tagaaggcgg tgttctcgat gccttcgagc
     4921 gcggtttcgc gcagcgtctt gtcggtgtcg ttcggcttca cgtccatacc ggcgaaccat
     4981 tgcgaggtcg cgcggtagat gatcggcgtc ttgtggcgcc agcagtgcat gtagctgtgc
     5041 gtgtacttct cggtgcgcat cagcgaaccg gcgttctgca gcgcctcgac gatctgtggg
     5101 ttggccgccc agatcgacag gccgccgaac agcgccagcg attcgatata gcggccgtca
     5161 cccatcaccg ggttgatgat gtccgaatcc gacatgccat gcgccttgca cgacacgaag
     5221 tcttctacgc catacgccgg cgacgagtgc acgacacccg tacccgtttc ggtcgtcacg
     5281 tagtcgccga gataaaccgg cgccgtgcgc ttgtagctcg gatgcgccgc ggcgagcggg
     5341 tgattgaagc gcaggttgac gagtttcgcg cccggcgtgg tcgcgatgac cgtgccttcg
     5401 aggccgtatt gcttcaggca ggcttcgacg cgttcttccg caagaatcag caggccgcgc
     5461 ggcgtatcga ccagcgcgta gacgatttcc ggatgcagat tcagcgcctg gttggcgggg
     5521 atggtccacg gcgtggtggt ccagatcacg atgccgcctt cattgcgcgg cagcgcaccc
     5581 aggccgaacg cctgcgcggt cttttccggc tcggcgaagc tgaatagcac gtcgatggtc
     5641 ggatcggtct tgtccttgta ctcgacttcc gcctcggcca acgccgagcc gcagtcgaaa
     5701 caccagttga ccggcttcag gccgcggaac acgtagccct tttccaagat cttcgcgagc
     5761 gcgcggattt caccggcttc gttcgtgaag ttcatcgtcc tgtacggatt gtcccagtcg
     5821 ccgagcacgc ccagacggcg gaagccgacc ttctgccttt caatctgttc ggtcgcgtag
     5881 gcgcgcgcct tctgcatcac ttcagcggcc ggcagcgatt tgccgaactg cttttcgatc
     5941 tggatttcga tcggcatgcc gtggcaatcc cagcccggca cgtagaccgc gtcgaagccc
     6001 gccagattgc gcgccttgac gatcatgtcc ttgaggatct tgttcaccgc gtggccgagg
     6061 tgaatgtcgc cgttcgcata cggcggcccg tcgtgcagga tgaacttctt gcggcctttg
     6121 ctggccgcac ggatcgtctc gtagaccttg cgttcctgcc actccttgac ccattgcggc
     6181 tcgcgtttgg gcaggtcgcc acgcatcggg aacggcgtgt cgagcaggtt gaccgggtag
     6241 cgtgactgcg gtttcgattc ggctttcttg ttgctcatga tgaggtcgcg gtgaattctt
     6301 tctaagcgca gcaaggctgc ggtaagcgca agatgcgcag ggcgtggggc gcgcccggca
     6361 ggcatgtcgc gtgatccacg gacgcccttc gatgcgcgca gcggtgggta catgcagcac
     6421 tcggtgcgct ggcatgtacc gcggcggcta tctaattcgg tcggtggccg aggtggcgaa
     6481 accggtggaa cggctgcccg gcgtgccggc gccgacggcc gcgaaccacg cgcgcgcgtt
     6541 gacgacgtcg cgcgagatgg cggcagtcag cgtttcgagg tcgacgtact tttcctcgtc
     6601 gcgcagcttc ttcaggaatt cgacgcgcac gagtttgcca tacgcgtcgc cgtgccagtc
     6661 gagcaagtgc acttcgagca gcacgcggcc cgaatcgtcg acggtggggc gcaggccgag
     6721 gcttgccaca cccggcaacg gaacgtccgc cacgccatgc acgcggacca cgaaaatacc
     6781 ggcgagcgcc ggacgcttgt gggcgatcgg cagattgagc gtggggaagc cgagatcgcg
     6841 gcccagcttc atgccgtgca cgacgtgccc gctgatcaga taatcgcggc ccagcgcggc
     6901 acgcgccgag tccagatcgc ccgccaccaa cgcggcgcgt acgcccgagc tggaaatgcg
     6961 cgcgccggac ggatcggcaa ccgtggccat ctgctcgact tcgaaaccgt gctgctgacc
     7021 cgcggccttg agcgacgcga aatcgcccgc gcgcttcgcg ccgtaacgga aatcgtcgcc
     7081 gatcatcacc cagcgcgcat gcagcccgtt gacgatgatc cgttcgacaa acgcgtccgg
     7141 cgactgactg gcgaacgtgt gattgaaatg ctcgaccacc acacgatcga cgccgttggt
     7201 gcgcagcgcc tcgagcttgt cgcgcagcat cgcgatccgc ggcggcgcac cggccgggtt
     7261 gaagaattcg cgtgggtgcg gctcgaaggt catcacacag acgggcaggc cgcgcgcgtc
     7321 ggctgcggcc cggacgtggg cgagcaaagc ctgatggccg cggtggacac cgtcgaagtt
     7381 gccgatggtc agtgcgcagg gcgcacggct ttcagcattg ggaagaccgc ggaagactct
     7441 cacgatagcg ggtttaaggt agggcgggcg aggtgccgca aaacaaacga ttataaacgc
     7501 tcagcgcacg agacggcggc gcgtcgccgt ttcagtgccc ctcgaatgat aaaatccgcg
     7561 gatgaaaaaa ctcgtcatcc tgatttccgg acggggaagc aatatggaag ctatcattcg
     7621 cgcctgcgcg gacgagggct gggcggcgca agtcgccgcc gtgattgcga accgtcctga
     7681 cgccgcgggc cttgcgttcg cggcgtcgca cggtattgcc acggcggtgg tcgaccaccg
     7741 ccagtttccg gatcgcgaca gtttcgacgc cgcgctcgcg caaaccatcg acagctttgc
     7801 gcccgatctg ctggtgctcg cgggcttcat gcgagtgctc acggccggtt tcgtcgatca
     7861 ttacgctggg cgcatgctga atgtgcatcc gtcgctgctg ccgagttttc cgggactgaa
     7921 aacccatcaa caggcattgg acgccggcgt gcggctgcac ggcgcgtcgg tgcattttgt
     7981 cacgtcgcag ctggatcacg ggccgatcgt cgcgcaggcg gccgtccccg ttgaggctgg
     8041 cgacaccccc gccatgctcg ccgaacgcgt gctggcaacc gaacacatta tttatccacg
     8101 cgcggtgcgc tggttcgtcg aagggcgtct tgctctcgac ggcttgcgtg tcacgcttac
     8161 gccgccggag ccgcaatggc tctttgccgg tcacaccgcc ggagagggcg catgagatta
     8221 catggttttt tgattggaca aaccgagact ttgctggctg aagtcctgaa actgaacggc
     8281 ccggccgacg ccacgacgag ccgttttttc cgcgcgcatc cgaagctcgg ccacggcgag
     8341 cgcggcgtga tcgccgaggc ggtcttcgca gtgctgcgcc gtcgcatgga attcgcccat
     8401 ctggctgagg gcggagcggg cagtccggcc cgccg
//
LOCUS       DATASH010000489         6723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2844732,
            whole genome shotgun sequence.
ACCESSION   DATASH010000489 DATASH010000000
VERSION     DATASH010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6723
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2844732"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(255..2501)
                     /locus_tag="VHV99_13525"
     CDS             complement(255..2501)
                     /locus_tag="VHV99_13525"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX3636486.1"
                     /translation="MSDVFVRVALDHPLPTLFDYRCDTPERVAPGMMVSVPFGKRHVV
                     GLVCEVTTHSDVPAERLRTVSNVCTACPPVSAEWLALTAFAADYYQRGLGEVALPALP
                     QALRDASRWSRLFAPAERYSLTAEGRAALPETLPARASALRKLAQALAEAEFLIAADA
                     RALHPKAIATLDAWQAAGWVALEVIEAAEVLANPASLNTPAPTLPTLTDEQASAVEAI
                     RDANGFAPFLLHGVTGSGKTEVYLRALAEILAAKPDAQALVLVPEINLTPQFEYAFRA
                     RFAALEGASIVTLHSGLAEGERARNWFAAHTGRARIVLGTRLAVLASLPKLAIIVVDE
                     EHDPAYKQQEGLRYSARDLAIYRAKQLGLPVVLGSATPSLESWWQADQGRYKRLTLSR
                     RAVAEAVLPTVKLIDLEEERRRGRASVEGLSGPLIAALKTRLERGEQSLVFLNRRGYA
                     PQLACDACGWVAGCPRCSAYVVLHKPERALRCHHCGWESRIPRSCPDCGNVDIAPMGR
                     GTQRVEETLASAVPGARVLRIDADSTRRKGSAQALFSDVHAGEVDILVGTQMIAKGHD
                     FQRVSLVGVLNADTALFSHDFRASERLFAQLMQVSGRAGRAGLPGEVLVQTRYPRHAL
                     YHALGRHDYVGFANSTLAERRDAHLPPFVYQALLRAEGRTLEAALAFLQQAAGALTEI
                     PAAERVTVYDAVPLTIVKVMHVHRAQLLIESASRGALQATLRAWQPMLRGLKGVLRWN
                     LEVDPLDI"
     gene            complement(2966..4066)
                     /gene="hemE"
                     /locus_tag="VHV99_13530"
     CDS             complement(2966..4066)
                     /gene="hemE"
                     /locus_tag="VHV99_13530"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEX3636487.1"
                     /translation="MAHKLLNDTFLRALLRQPTDYTPIWLMRQAGRYLPEYNATRGRA
                     GSFLGLAKNPAYATEVTLQPLERYPLDAAILFSDILTVPDAMGLGLEFVAGEGPKFAR
                     PVRTEDDVARLAVPDIDATLRYVTDAVREIRTALTDAQGRQRVPLIGFSGSPWTLACY
                     MVEGGGSADFRTVKSMLYARPDLMHRILDVNAKSVAAYLNAQIEAGAQAVMIFDTWGG
                     SLADGVYQRFSLQYIQQVVSQLKRDHDGEKVPVITFTKGGGLWLDEIAEIGVDAVGLD
                     WTVNLGKARERVGSKVALQGNIDPSVLFAPPASIRMEARAVLDSFGNHPGHVFNLGHG
                     ISQFTPPENVAELVDEVHRHSRAIRSGAHAPT"
     gene            4280..5077
                     /locus_tag="VHV99_13535"
     CDS             4280..5077
                     /locus_tag="VHV99_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX3636488.1"
                     /translation="MNKTALALAGVLAGILMQTGVAQAQTSATAPAPSRLDEILARGT
                     LRACTTGDYKPYSFYKADGQFEGIDIDMAESLAKSLGVKAEFVKTSWSNLMNDFVAKC
                     DVGVGGVSPTLERQKRAFFTQAYMVDGKTPIVRCDDVNKYQTVAQIDQPSTRVIVNPG
                     GTNERFAKQYFPHANLTVYPDNVTVFKQILAGKADVMVTDASETMLQQKLNPGLCSVH
                     PDKPFQYGEKAWLLPRGDMAFQQYVDQWLHLAQATGEYQTISDKWLK"
     gene            complement(5963..>6723)
                     /locus_tag="VHV99_13540"
     CDS             complement(5963..>6723)
                     /locus_tag="VHV99_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015004080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX3636489.1"
                     /translation="NFATYDFSRLRTGIMAGSPCPIETMKKVVSTMHLSEITIAYGMT
                     ETSPVSFQSSTADPLDKRTTTVGRIQPHLEVKIVDPLGEIVPVGETGELCTKGYSVMQ
                     GYWGDEAKTRESIVDGWMHTGDLATLDADGYCNIVGRLKYMLIRGGENIYPREIEEFL
                     FRHPKIQSVQVFGVPDAKYGEEVCAWIVLRAGEQATAEEIQQFCQGQIAHYKIPKYIR
                     FVDELPMTVTGKVQKFVMRERMISELKVQEDKTA"
BASE COUNT         1277 a         2066 c         2098 g         1282 t
ORIGIN      
        1 agaccgccgc cgtgcgcgct atggccgctg cgcggttggc tgggcgcggc gccgctcggc
       61 agtgtctggt tggtatcgct catcgcacat gccccgggaa aattaaggtg catcggcgcg
      121 tgatgcttga gttttgcccg aatatgcgcc gattcggcga aatataggcg agcggcggcg
      181 caaagcgaaa cgaccattcg ctctatgaat atgactgaaa ggagtaacgc tctgcgctga
      241 cgcggcgagc ggcgtcagat gtcgagcggg tcgacttcga gattccagcg cagcacgcct
      301 ttcaggccgc gcagcatggg ttgccacgcg cgcaaagtgg cctgcaatgc gccacgcgac
      361 gcgctctcga tcagcaattg cgcgcgatgc acgtgcatga ccttgacgat ggtgagtggc
      421 acagcgtcat agaccgtcac gcgctcggcg gccggaatct cagtcagcgc gccggccgcc
      481 tgctgcagga aggcgagcgc ggcttcgagc gtgcggcctt cggcgcgcag caaagcctga
      541 tagacgaacg gcggcagatg cgcgtcgcgc cgctcggcca gcgtcgaatt ggcaaagccg
      601 acgtagtcgt gacgccccag cgcgtgatac agcgcgtggc gcggatagcg tgtctgcacc
      661 agcacttcgc ccggcaaacc ggcgcggccc gcgcgcccgc tgacttgcat caattgcgcg
      721 aagaggcgtt cgctcgcgcg gaagtcgtgt gaaaacaagg cggtgtcggc gttcagcacg
      781 ccgaccagcg acacgcgctg gaagtcgtga cccttggcga tcatctgcgt gccgacgagg
      841 atgtcgacct cgccggcgtg gacgtcggaa aacagcgcct gcgcgctgcc tttgcggcgc
      901 gtgctgtcgg cgtcgatgcg cagcacgcgg gcgccgggta ccgcgctggc cagggtttcc
      961 tcgacgcgtt gcgtgccgcg tcccatgggc gcgatgtcga cattgccgca gtccggacac
     1021 gagcgcggaa tgcgcgattc ccagccgcag tggtggcagc gcagcgcgcg ttcgggtttg
     1081 tgcaggacga cataggcact gcagcgggga cagccggcga cccagccgca ggcgtcgcag
     1141 gcgagctgcg gcgcgtagcc gcgacggttc aagaacacga gactttgctc gccacgctcg
     1201 agccgtgtct tcaacgcagc gatcagcggt ccggacaacc cctccaccga ggcccgcccg
     1261 cgccgccgct cctcttccag atcgatcagc ttgacggtcg gcagcacggc ctcggcgacc
     1321 gctcggcgcg acagggtgag acgcttgtag cgtccctgat ccgcctgcca ccagctttcc
     1381 agcgacggcg tggccgaacc gagcacgact ggcaaaccga gttgcttggc gcgatagatc
     1441 gccagatcgc gcgccgaata gcgcagacct tcctgctgct tgtaggccgg atcgtgttcc
     1501 tcgtcgacga cgatgatcgc gagcttgggc agcgaggcaa gcaccgccag ccgcgtgccg
     1561 agcacgatgc gggcacgacc ggtgtgcgcg gcgaaccagt tgcgggcgcg ttcgccttcc
     1621 gcgaggccgc tgtgcagcgt gacaatcgac gcgccttcga gcgccgcaaa gcgcgcacgg
     1681 aaggcgtact cgaactgcgg cgtcagattg atttcaggga cgaggacgag tgcctgcgcg
     1741 tcgggcttcg ccgcgaggat ctcggcgagc gctcgcagat acacctccgt cttgccactg
     1801 cccgtcacgc cgtgcagcag gaacggcgcg aaaccgtttg cgtcgcggat cgcttcgact
     1861 gcgctcgcct gttcgtcggt gagggtgggt agcgtgggtg cgggcgtatt cagcgatgct
     1921 ggattggcaa gcacctcggc ggcttcgatc acttccagcg cgacccagcc tgcggcttgc
     1981 caggcgtcca gtgtcgcgat ggctttggga tggagcgcgc gagcgtccgc ggcgatcaga
     2041 aattcggcct cggccagagc ctgggcaagc ttgcgcaacg cgctggctcg cgccggcaac
     2101 gtttcgggca atgcggcgcg accctcggcc gtcaagctgt aacgttccgc cggcgcgaat
     2161 aggcgcgacc accgcgaagc atcacgcagg gcttgcggca aagcgggcag cgccacttcc
     2221 ccgaggccgc gctgatagta gtcggcggcg aaggccgtga gcgccagcca ttcggccgat
     2281 acgggcgggc aggctgtgca gacattgctg accgtccgca agcgctcagc aggcacgtca
     2341 ctgtgagtgg tcacttcgca gacgaggccc accacgtggc gtttgccgaa cggcacgctc
     2401 accatcatgc ccggtgcgac acgctcgggc gtgtcgcacc ggtagtcgaa caaagtcggc
     2461 agcggatgat ccagcgcgac gcgaacgaat acgtcactca cgcggcggca cggactgggt
     2521 aaatggatgc agcacgacgc cacgtcatgg tgcacattga acaggcggtg aagccatcga
     2581 agttaaagta aaacttcaat ttcggcgcta agttttggat tcggctgaac aattgcgatc
     2641 ggtccgcttg cctgtggata actttgttga gaacttcgtc cagaagggct ggaaaccgcg
     2701 ccgcagcgcc cttctgtggg attccgcaca ttttgtcgta accccttgaa acccttgcca
     2761 gacaaggctt agatcaaatg cgcctgcaat gtgcaagggc tggaacacga cgaaagcttc
     2821 taccgcgccg cagcgtgacc aatgtgcata agtcaagtct tgactttcgc gggaccgcca
     2881 tctgacggcc cattcactgc aatgtttccc ttagggcgag agcccagctc gctgcgccgc
     2941 agcaaaaatg gtaacgatta cggcatcagg ttggtgcgtg cgcgccgcta cgaatcgcgc
     3001 ggctatgccg atggacttcg tccacgagtt cagcaacatt ttccggcggc gtgaactgcg
     3061 aaatcccatg gccgagatta aaaacatggc caggatgatt gccgaagctg tcgagcacag
     3121 cgcgcgcttc catgcggatc gaagcgggcg gcgcaaacag caccgaagga tcgatattgc
     3181 cttgcagcgc caccttgcta cccacacgct cacgcgcctt gcccaggttc acggtccagt
     3241 cgagaccgac cgcatccacg ccgatctcgg cgatctcgtc gagccacaac ccgccgccct
     3301 tggtgaaggt gatcaccggt actttttcgc cgtcgtggtc gcgtttcagc tggctcacga
     3361 cctgctggat gtactgcagc gagaagcgct ggtagacgcc gtccgcgagt gacccgcccc
     3421 acgtgtcgaa gatcatcacg gcttgcgcgc cggcttcgat ctgcgcattc aggtacgccg
     3481 caaccgactt tgcgttgacg tcgagaatac ggtgcatcag atccggtcgc gcgtacaaca
     3541 tcgatttgac ggtgcggaag tctgccgagc cgccgccttc gaccatgtag caggccagcg
     3601 tccaggggct gccggaaaaa ccgatcagcg gcacacgctg acggccttgc gcgtcggtaa
     3661 gcgcggtgcg gatttcgcgc acggcgtcgg tcacatagcg cagcgtggcg tcgatatccg
     3721 gcaccgcgag gcgcgccaca tcgtcttccg tgcgcaccgg ccgggcgaat ttcgggccct
     3781 cgccggcaac gaactcgaga cccaggccca tcgcgtccgg caccgtcagg atgtcggaga
     3841 acaggatcgc ggcgtcgagc ggatagcgct cgagcggctg cagcgtgact tccgttgcgt
     3901 acgccggatt ctttgccaga ccgaggaaac tgcccgcgcg cccccgtgtg gcgttgtatt
     3961 ccggcagata gcggccagcc tgccgcatca gccagatcgg cgtgtagtca gtcggctggc
     4021 gcagcagcgc gcgcaggaaa gtgtcgttca ggagtttatg ggccacggtg cgtacgacag
     4081 aaggcaaaag ggcattttac cggacgcggc ttgtcatcgc gcttgatgcg ggtaataact
     4141 gtcgcgcacc ttgctcgcgt ggcctgttgc ggctccttcc ggcgccgtgc ctcagctgaa
     4201 cggccaatgt tcaagcgcgc gttggcgggc tatcatccga cggctttgcc aaacaacaac
     4261 gaacaactaa ggagactcaa tgaacaagac agcactcgca ttagccggcg ttctggccgg
     4321 cattttgatg cagacgggcg tcgcccaggc acagaccagc gcaaccgccc cggcgccttc
     4381 gcggctcgac gaaatacttg cgcgcggcac cctgcgcgcc tgtacgaccg gtgactacaa
     4441 gccgtactcg ttctataaag cggacggcca gttcgaaggc atcgatatcg acatggccga
     4501 gtcgctcgcc aagtcgctcg gcgtaaaggc ggaattcgtc aagacctcgt ggtcgaacct
     4561 gatgaacgac ttcgtcgcga aatgcgacgt cggcgtaggc ggtgtttcgc cgacgctcga
     4621 gcgccagaag cgcgcgtttt tcacgcaggc gtatatggtc gacggcaaga cgccgatcgt
     4681 tcggtgcgac gacgtcaaca aatatcagac cgtggcgcag atcgatcaac cgtcgacccg
     4741 cgtgatcgtc aatccgggcg gtactaacga gcgtttcgcc aaacagtatt tcccgcatgc
     4801 aaacctgacc gtctatccgg acaacgtgac ggtcttcaag cagattcttg ccggcaaggc
     4861 cgatgtgatg gtgacagacg cgtcggagac catgttgcag caaaaactga atccgggtct
     4921 ttgctcagtg catccggaca agcccttcca gtacggtgag aaggcctggc tgctgccgcg
     4981 tggcgacatg gcctttcagc aatacgtcga ccagtggttg caccttgcgc aagcgaccgg
     5041 cgagtatcag acgatctcgg ataagtggct gaagtgaagc gggagttgca gggttcggcg
     5101 cgcgtcgcgc tcgatcggga aaagcaggtg gggctgatcg cttataggtt cccgtggtta
     5161 gcgcaatcgt gtaaccggac ctcaaggtat taagtaaaac tcgaacaatg cggtttgaat
     5221 gcggggtcaa cgaggcggcc cctttctcat ctttcttccg catgctccaa ttgttgcaac
     5281 gcagcgcaac cgctcgacag caaaccgaag gtttcgtggt ggtcgaaaag cgcttgtaac
     5341 acggtcgatt cgtcgcaaaa aagatgagca tgcaactatt tcatgcggaa tcgactgaac
     5401 ggattacgcc gcaatatgcg tcgcgaatag catcaatcag gcgtatgaaa ttgcgcgcca
     5461 gtcatcggta aaaattgcgt ttgaaacgct tgcaattggc aataaatccc ggggcgcctt
     5521 atctggcggg cgttacaacc tgaaacactt tgttaccgcg ctcgtaggtt aattcgccca
     5581 caatgccttc aacggtttca acacggatgt ggctaggtgc gtggtgtgag cggatacgat
     5641 gcacttgccg gtcggcgtat gccgaagccc tgtgaagggg catccctgct ggggccgtag
     5701 tcggcgcagg gtcgtcgtct cagtcggttc caactttggt ctcctcgcgc taaccccgta
     5761 gcgtgtggtt tttagcgggc tccaggcccg cttttttttc gcccggtcaa acgtttgcga
     5821 gctgcttgtt ggcggatcga ttttgcaggt tttcaaccgc gcaatttccg ccctcgcacg
     5881 ccggtttcgc tgcaaacggt tttccccaaa atgccggccg gcgccgtgcg cattttcgtg
     5941 cggcgccgaa tcagcatggc cgtcaggccg tcttgtcttc ctgcaccttc aattcgctaa
     6001 tcatccgttc gcgcatgacg aatttctgga ccttgccggt caccgtcatc ggcaattcgt
     6061 caacgaagcg gatgtacttc ggtatcttgt agtgtgcgat ctggccctgg caaaattgct
     6121 gaatctcctc tgccgtggcc tgctcccccg cgcgtaaaac gatccacgca cacacttcct
     6181 ctccgtactt cgcgtcgggc acaccgaaca cctgcacgct ctggattttc ggatgccgga
     6241 acagaaactc ctcgatctcc cgcgggtaga tgttttctcc gccgcgaatc agcatgtact
     6301 tcagtcggcc aacgatgttg cagtagccgt cggcatccag cgtcgccaga tcgccggtgt
     6361 gcatccagcc gtcgacgatg ctttcccgcg tcttcgcttc gtcgccccaa tagccttgca
     6421 tcaccgaata gcctttggtg cacagttcgc ctgtttcgcc caccggcacg atctcaccga
     6481 gcggatcgac gatcttcact tcgagatgcg gctggatgcg gccgaccgtc gtggtgcgct
     6541 tgtcgagcgg atcggcggtc gaactctgaa acgacaccgg gctagtttcg gtcatgccgt
     6601 acgcgatggt gatttcgctc aaatgcatcg tggaaaccac tttcttcatg gtctcgatcg
     6661 ggcacggtga gccggccatg attccggtgc gtaggcgcga aaagtcgtag gtggcgaagt
     6721 tcg
//
LOCUS       DATASH010000490         2218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6999822,
            whole genome shotgun sequence.
ACCESSION   DATASH010000490 DATASH010000000
VERSION     DATASH010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6999822"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(319..1062)
                     /locus_tag="VHV99_13545"
     CDS             complement(319..1062)
                     /locus_tag="VHV99_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEX3636490.1"
                     /translation="MKLLIVNPNISSSVTHLIEAEARRVALADTQIEMATAASGVAYI
                     ETRFEALLGAHAVANIAGERKGEYDALLVAAFGDPGLLELREVLDVPVVGMTEAALMT
                     AAQLGQRIGIIAISRRITAWYRECVDRYGMLGRLAGIRHLDRPLRDPGTVQEDHGAHL
                     IELSHRAVEEDGADVLILAGAPLAGLARSVADRLPVPVVDGVSSGVCQAQALTRLATA
                     RAQAGSFAKLPAKPNAGLSGGLATLLLRE"
     gene            complement(1124..1852)
                     /locus_tag="VHV99_13550"
     CDS             complement(1124..1852)
                     /locus_tag="VHV99_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX3636491.1"
                     /translation="MLTDKASLRPRKARPAAQILDAEDNSDTIYERILTAVMEHRLIP
                     GTQLVEEKLAAVFKVNRTRIRKVLARLTHEGIVTTIPNRGAFIASPSVQDARHIFQAR
                     RILEPALVRSLVERSSAEGFRRLHAHVAKERAAREKGDKHAVIRLSGEFHLLIAEMVG
                     SPPLTKLMRELASLTCLIILLFDSPNVPACPNHEHTEIVGLMEKGELEAAIAAAIGHL
                     DHIEATLDLSLPGDTEVDLNAIFG"
BASE COUNT          427 a          705 c          650 g          436 t
ORIGIN      
        1 ggttcgcacc gaaagcaaat gccccgagga ccgagcgtct gcataccgac aattgcgcgt
       61 atgaaatgaa ctgctcgccc aacacagcgt acctgccaac gacgctatct cctatgccgg
      121 ctaccgattt cctttaatcc ggccactttc gaggatcgct tcgagaccat cgagcgggtc
      181 ttcgcctggg aggagaagcc ccggcccctg cgcgctcacg ccacatcacc tctccacccc
      241 gattgcgcta gcgcgaaacc cgcgaccagt taagaagcta tcgcataagt tcaactggaa
      301 ttcgcgcccc agcgtctctc attcacgcaa tagcaatgtg gccaggccac ctgatagccc
      361 ggcattgggc tttgccggca acttcgcgaa actgccagcc tgcgcgcgcg cggtggcgag
      421 ccgggtcaac gcctgcgcct gacacactcc gctcgatacg ccatcgacca ccggcaccgg
      481 cagccggtcc gccacgctgc gcgccaatcc cgctagcggc gcgccggcta gaatcagcac
      541 gtcggcgcca tcttcctcca ccgcacgatg gctcagttcg atcaggtgtg cgccatgatc
      601 ctcctgaacc gtaccgggat cgcgaagcgg ccggtcgaga tgacggatgc ccgccagccg
      661 gccgagcatg ccataacggt ccacgcattc gcgataccag gccgtgatgc gtcgcgaaat
      721 cgcaatgatg ccgatccgct gaccgagttg ggcggcggtc atcagcgccg cttcggtcat
      781 gccgacaacc ggcacgtcga gcacttcgcg caactcaagc aggcctgggt cgccgaacgc
      841 cgcgaccagc agcgcatcat attcgccctt gcgctcgccg gcgatgttcg ccaccgcgtg
      901 cgcgcccagc agcgcctcga aacgggtttc aatgtaggcg acgccgctcg ccgccgtcgc
      961 catctcgatc tgtgtatcgg ctaaagcgac gcggcgcgct tcggcttcaa tcaaatgggt
     1021 gacgctcgac gagatgttcg ggttgacgat cagtagtttc atcacggttc cagtcaggtg
     1081 caatggcgca attgccatgt ttaagagcga aggcgaggcg cgttcatccg aagatggcgt
     1141 tcagatcgac ctcggtgtct ccaggcaacg acagatcaag cgtcgcctcg atgtgatcga
     1201 ggtgtccaat cgctgccgcg atcgccgcct ccagttcgcc cttctccatc agcccgacga
     1261 tttccgtgtg ctcatggttc gggcaggccg gcacgttcgg cgaatcgaac agcaagatga
     1321 tgaggcaggt cagcgacgcg agttcgcgca tcagtttggt gagcggcggg ctccccacca
     1381 tctcggcgat tagcagatga aattcaccgg agaggcgaat caccgcatgc ttgtcgccct
     1441 tttcgcgcgc ggcgcgttcc ttggcgacgt gcgcatggag tcgccggaaa ccttcggcgc
     1501 tgctacgttc gaccaggctg cggactagcg ccggctcgag aatgcgccgc gcctggaaga
     1561 tatggcgggc gtcctgcacc gacgggctag cgatgaacgc cccgcgattc gggatggtgg
     1621 tgacgatgcc ttcgtgcgtg agccgcgcca gcaccttgcg gattcgcgtg cggttcacct
     1681 tgaacacggc ggcgagcttt tcctcgacga gttgggtgcc cggaatcagc cggtgctcca
     1741 tcacggcggt caggatgcgc tcgtagatcg tgtcggaatt gtcttccgca tccaggatct
     1801 gggcggccgg ccgcgcctta cgcgggcgga gggatgcttt gtcggttaac acgctaggtt
     1861 tcctcgactg accaacggtc attttgtgtc aatacgatta tatattgtga caaaaataat
     1921 tgtaattgtt cgaatttgca ccatcagcgc acaccggcac cgcttacgta tcccaaaggc
     1981 accggaacgg tgccaggttc gtgcgcgcgg cgggaatccg gtttcgccgg ccggctggcg
     2041 atatggatcg cgtcgttttg cctcacagta cctgtaacgc ggatacacgc ggccacgcgg
     2101 acttaagcca tgtacaagca tatcgctcga agtcgctgga gacggcctgc caatcgattg
     2161 gcacgactat tgctttattt gtcacaaaca tatttcattt tgtgacaata acggagct
//
LOCUS       DATASH010000491         2858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5813542,
            whole genome shotgun sequence.
ACCESSION   DATASH010000491 DATASH010000000
VERSION     DATASH010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5813542"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VHV99_13555"
     CDS             <1..237
                     /locus_tag="VHV99_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctA"
                     /protein_id="HEX3636492.1"
                     /translation="PVLGLVLILPVDWFVGIARALTNLIGNCVATVVVAVWENDIDRV
                     RAHRVLNRDEALRYVPAGEDASAEPTAGAHVPAV"
     gene            580..1590
                     /gene="alc"
                     /locus_tag="VHV99_13560"
     CDS             580..1590
                     /gene="alc"
                     /locus_tag="VHV99_13560"
                     /EC_number="3.5.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006025245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004037 - allantoicase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoicase"
                     /protein_id="HEX3636493.1"
                     /translation="MANPILDPNAPAFTRRYMNLADPRLGAQALYASDEFFAPKERML
                     EPQPAVFIPGKYDDHGKWMDGWETRRKRTTGHDYCVIRLARPGVVHGVDLDTSHFTGN
                     FPPAASIDACYVDGDVPPDNADWQTLVQATTLQGNHHHYVEVNDARAFTHLRVNLYPD
                     GGLARLRVYGQPKRDWERVERGTLLDLAAIENGAYLVAANNQHFGPASQMLMPGRGVD
                     MGDGWETRRRREPGNDWAIVALARPGVIRKIDVDTAHLKGNYPDRCSLQAASVTGGTD
                     DSLITQAMFWPVLLGEQKLQMDHVHSFADDIAPLGPVTHVRFNIFPDGGVSRLRLWGE
                     IV"
     gene            1604..2113
                     /locus_tag="VHV99_13565"
     CDS             1604..2113
                     /locus_tag="VHV99_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HEX3636494.1"
                     /translation="MKTLQMERLTRAAFAPFGELIELDGARHFPINGGTTERYHDLAN
                     VDVTENGGRALINLFRAQPRALPIEITMMERHSLGGQAFIPLTARRYLVVVAPPGECD
                     PARMRAFWSDGWQGVNYAKGVWHHPLLALDQVSDFVVVDRGGEQPNCDEIPLTEPWRL
                     EFKASVELA"
     gene            complement(2363..2791)
                     /gene="fur"
                     /locus_tag="VHV99_13570"
     CDS             complement(2363..2791)
                     /gene="fur"
                     /locus_tag="VHV99_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HEX3636495.1"
                     /translation="MTNPTDLKNIGLKATLPRLKILEIFQHSPVRHLTAEDVYRNLLH
                     EELDIGLATVYRVLTQFEQAGLLSRSNFESGKAVFELNEGSHHDHLVCLDCGLVEEFF
                     DSEIESRQQSIAKERGFKLQEHALALYGVCTKENCPHRKH"
BASE COUNT          527 a          924 c          892 g          515 t
ORIGIN      
        1 cccgtgctcg gcctcgtgct gattctgccg gtcgactggt tcgtcggcat tgcccgcgcg
       61 ctgactaacc tgatcggcaa ttgcgtggcg accgtggtgg ttgccgtgtg ggaaaacgat
      121 atcgaccggg tgcgcgcgca tcgcgtgctg aaccgcgacg aagcattgcg ctatgtgccg
      181 gcaggcgaag acgcgagcgc cgagcccacc gccggcgcgc acgtgcccgc cgtttgataa
      241 gagcagtctg ataagagcag tccgagaccc cgctgcctga cgcctgcgct tcggaaccgg
      301 ctccccggga ccagcaccct gatgttggtg cttcggaacc ggcgtctcag acccagcacc
      361 tcagacccag cacctcagaa ccagcacctc agaaccagca cctcagaccc agcactccga
      421 tgccggcgct tcacaacagg cgcccaagaa ccagcagtcc gatgccgctg cttcagagcc
      481 ggcagtccaa tccagcgcgc cgaagcggcg cgcgccttgc cccggcagat gcgttgactc
      541 cgccggctct tcctcacttt tcgttgcctg accaagacta tggctaatcc gatccttgac
      601 cccaacgctc ccgcattcac ccgtcgctac atgaacctcg ccgacccgcg tctgggcgcg
      661 caggcgctct acgcgagcga tgaattcttc gcaccgaaag agcgcatgct cgaaccgcag
      721 ccggcggtgt ttatccccgg caagtacgac gaccacggca aatggatgga cggctgggag
      781 acgcgccgca agcgcaccac cggccacgac tattgcgtga tacggctcgc gcgaccgggc
      841 gtcgtgcatg gtgtcgatct ggacacgagc cacttcaccg gcaatttccc gccggccgcg
      901 tcgatcgacg cgtgctacgt agacggcgac gttccccccg acaatgccga ctggcaaacg
      961 ctcgtgcagg ccaccacgct gcagggcaac caccaccatt acgtcgaagt gaacgacgcc
     1021 cgcgctttta cccatctgcg cgtgaatctg tatccggacg gcggtctcgc gcgcctgcgc
     1081 gtgtacggcc agccgaagcg cgattgggaa cgcgtcgagc gcggcacgct gctggatctc
     1141 gccgcgatcg aaaacggcgc gtacctcgtc gctgcgaaca accagcattt cgggccggcc
     1201 tcgcagatgc tgatgccggg ccgcggcgtc gatatgggcg acggctggga aacgcggcgc
     1261 cgtcgcgagc cgggcaacga ctgggcgatc gtcgcgctcg cacggccggg cgtgattcgc
     1321 aagatcgacg tggatacggc gcacctcaag ggcaactacc ccgaccgctg ttcgttgcag
     1381 gcggcctcgg taacgggcgg cacggacgat tcgctcatca cgcaggcgat gttctggccg
     1441 gtgctgctcg gtgaacagaa gctgcagatg gaccacgtgc acagcttcgc ggacgacatc
     1501 gcgccgctcg gaccggtgac gcacgtgcgt ttcaatatct tcccggacgg tggcgtgtcg
     1561 cgcctgcgtt tgtggggcga gatcgtataa cggagtcacg tcgatgaaga cattgcagat
     1621 ggaacgtctg acgcgcgcgg ctttcgcgcc gttcggcgag ctcatcgaac tggacggcgc
     1681 gcgtcatttt ccgatcaacg gtggcacgac cgagcgctat cacgacctcg cgaacgtcga
     1741 cgtgacggag aacggcggcc gggcgctgat caatctgttt cgggcgcagc cgcgcgcgct
     1801 gccgatcgaa atcacgatga tggaacggca ttcgctgggt ggccaggctt tcattccctt
     1861 gacggcgcgc cgctatctgg tggtcgtggc cccgccgggc gagtgtgatc cggcgcggat
     1921 gcgagcgttc tggagcgacg gctggcaggg cgtcaactac gcgaaaggcg tttggcacca
     1981 tccgctgctc gcgctcgatc aggtgagcga ttttgtcgtg gtggatcgcg gtggcgagca
     2041 gcctaactgc gacgaaatac cgctgaccga gccgtggcgg ctcgaattca aagcgagcgt
     2101 cgagttggcg taaaacgccg aagtcgccac gcagcggcaa tacgccacat gcaagtctgt
     2161 tcggacaact cgatcagctt gcccgcgctc gcgatgcccg gcaccgatcg gcccgctcgc
     2221 ggaccgacgc atcggcctcg aggctagcgt ccccgcgcgg cagcgtcgcg cataaaaaaa
     2281 cccggccgat tttcgtcgac cgggtttctg tagcccaccg aaccgcgcac cccgacgggg
     2341 ccggtagcgc accaattccg cattaatgct tgcgatgcgg acaattctcc ttggtacaga
     2401 cgccgtacag cgccaacgca tgttcctgca gcttgaagcc gcgttccttc gcgatggatt
     2461 gctggcggct ctcgatctcg gagtcgaaaa actcttcgac gagcccgcaa tcgagacaga
     2521 cgaggtggtc gtgatgcgac ccttcgttca gttcgaacac cgccttgccc gattcgaaat
     2581 tgcttcgcga gaggaggccg gcctgctcga actgcgtcag cacccgatac acggttgcga
     2641 gaccgatatc gagttcctcg tgcaacaggt tgcggtagac gtcttctgcc gtcagatggc
     2701 gcactgggct atgctggaaa atctcaagga ttttgaggcg cggaagggtc gccttgagcc
     2761 cgatattctt gagatcggtt ggattggtca tgacaaggga tccctagagt acaatgctgg
     2821 gctctcatag taatgtgttt ttgccgttct ggtcatct
//
LOCUS       DATASH010000492         5145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5218418,
            whole genome shotgun sequence.
ACCESSION   DATASH010000492 DATASH010000000
VERSION     DATASH010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5145
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5218418"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="VHV99_13575"
     CDS             <1..900
                     /locus_tag="VHV99_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEX3636496.1"
                     /translation="RLDHPHDVWLAATQARDGVRFVFGLAGCPPVMANANTGALAAVL
                     PSQVAALVRALLHTFLDLAAADATRMRHVLTAHSVDAVLQHAQTYVDFPLSRDTSLAN
                     WQRGAPADAMLRLGAHAQRVVGMLHVGGQPPLGRLDAATLRSLATLSQQQGNGTLHMT
                     PWQSVLLPDIAAHAAPAVLAEMNMLGLACDPAQSMTHLIACAGSTGCVKGLADTKADA
                     QLLADRLPNGVDVHLSGCPRSCAAAHCAPYTLLAAAPGIYDLYRRDGQPGFGSCVARQ
                     LTIEQAADLLGPLAHPTRSPLDA"
     gene            893..1519
                     /locus_tag="VHV99_13580"
     CDS             893..1519
                     /locus_tag="VHV99_13580"
                     /EC_number="5.4.99.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017778279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016993 - precorrin-8X methylmutase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HEX3636497.1"
                     /translation="MLDYIRDGQEIYRQSFATIRAEADLSRIPADLEKLAVRVIHACG
                     MVDVIEDLRFSEAAGAAGRTALAQGAPILCDAGMVAQGITRARLPANNEVICTLTHQN
                     VPALARELGNTRSAAALELWRPYLAGSVVVIGNAPTALFHLLDMLDAGAPKPALILGF
                     PVGFVGAAESKALLAEDSRGVPYVVVHGRRGGSAMAAAAVNALATEVE"
     gene            1520..2251
                     /locus_tag="VHV99_13585"
     CDS             1520..2251
                     /locus_tag="VHV99_13585"
                     /EC_number="2.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015601722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030788 - precorrin-2
                     C20-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-2 C(20)-methyltransferase"
                     /protein_id="HEX3636498.1"
                     /translation="MTVQGRLFGLGVGPGDPELITLKALRLLKAAPVVAYFVAKGKKG
                     NAFSIIEAHLHDAQQHLPLVYPVTTEALEPPLSYEAIIADFYDTAAQIVADHLDAGRD
                     VAVICEGDPFFYGSYMYLHDRLAPRFEAEVVPGVCSMLGGAAVLGAPLVYRNQSLSVL
                     SGVLPEDELRRRLADADAAVIMKLGRNFDKVRRVLGELGLAHRALYVERATMGNQRIV
                     PLAEVDPMASPYFSLLVVPGEKWQG"
     gene            2248..4086
                     /gene="cobJ"
                     /locus_tag="VHV99_13590"
     CDS             2248..4086
                     /gene="cobJ"
                     /locus_tag="VHV99_13590"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030789 - precorrin-3B
                     C17-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HEX3636499.1"
                     /translation="MMPPAIVVLGAGALATARRIQALYAGCEVHALRGRVEADVAYTE
                     LGAHLRELYARGTPIVALCAAGIVIRCVGPLLSNKGVEPPVLAVAEDGSAVVPLLGGL
                     AGVNVMAREIAAALGVSPAITTSGELRFGTCVLNPPDGYALADIVQGKRFVSDLLAGE
                     STRIEGEAPWLDDAQLPRSDSARLAIRVTPRASRGRDDELVIHPRSVVAAVTVGGAGT
                     DAGASASASAGAGASAGGGTIFATAAGPTNSSPEAQSHSGEQIAARVREALDAHGLAA
                     LSLAALLASSEQMANPAFADAAASLNVPLRFTQIRPEDGEPPGNLLHAALRIPYETLH
                     KDTNTGVALALAPLAIDADTIGRPRGRLTVIGLGPGSADLMVPAARTALNEATDILGY
                     ETYVKMAGPFRADQRVDGTDNREEMQRARHAFELASTGRSVVMVSSGDPGVFAMAAAV
                     LEALEEGSSTNADWRAVQLAIVPGISAAMATAAQAGAPLGHDFCMLSLSDNLKPWTII
                     EKRLRHAAEADLVMAFYNPISRARPWQLDKALDIVREYRAAATKVVLGRDIGRPGSTL
                     RTTTLGELRSSDVDMRTMVIVGSSTTRGFANGIDGGEWVYTPRWYE"
     gene            4432..5121
                     /locus_tag="VHV99_13595"
     CDS             4432..5121
                     /locus_tag="VHV99_13595"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEX3636500.1"
                     /translation="MMELRRAAADYQSRQQSDAAELIRSRQKQTDLELKIATLRNDYV
                     KSADSQLKDTTAKILQITEQLRPAQDVDTRTRIVAPVAGEVVGLRVHTIGASIGPREP
                     ILDLVPTGVPLIVEAKIKPDNVREIVPNSKADVRLTAYNPRTSPVLEGTVTYLSADAL
                     NDKDTRQPFYLAHIEVSAKAIAKANRLAKEPIVLGPGLRAEVFIKTRSRSAFDYLFEP
                     VWDGIQRSMRD"
BASE COUNT          928 a         1574 c         1631 g         1012 t
ORIGIN      
        1 aggctcgatc atccgcatga tgtctggctg gcggcaacgc aagccagaga cggtgtgcga
       61 tttgtgtttg gtttggcggg ttgtccgcct gtgatggcga atgcaaatac cggcgccctc
      121 gcagccgtgc tgccatcgca agtcgccgcc cttgtgcgcg cactgctgca tacgttcctc
      181 gatctcgccg ccgccgatgc cacgcgcatg cgtcacgtgc ttaccgcaca ttccgtcgac
      241 gccgtgttgc agcacgctca aacgtacgtc gatttcccgc tgtcacgcga cacgtcactt
      301 gcaaactggc aacgcggcgc gcctgccgat gccatgctgc gactcggcgc gcacgcgcag
      361 cgagtcgtgg gaatgctgca tgtaggcggc caacccccgc tcggacgcct cgacgcagcg
      421 accctgcgca gcctcgccac gctctcgcaa cagcaaggca acggcacgct ccacatgacg
      481 ccctggcaaa gcgtgttgct gcccgatatc gctgcccatg ccgcgcccgc cgtgctcgct
      541 gaaatgaaca tgctaggcct tgcctgcgac cccgcccagt caatgacgca cttgatcgcc
      601 tgcgccggtt ccacaggctg cgtcaaaggc ctcgccgata ccaaagccga cgcgcagctt
      661 ctagcggatc gcttgcccaa cggcgtcgac gtccatctca gcggctgtcc gcgctcgtgc
      721 gccgccgccc actgcgcgcc atacacgctg ctggccgccg cgcccggcat ctacgacctt
      781 tatcgacgcg acggccagcc aggtttcggt tcgtgcgtcg cgcgccaact cacgatcgaa
      841 caggccgccg acctgctcgg cccattggca cacccgaccc ggagcccttt agatgcttga
      901 ttacattcgc gacggtcagg agatctatcg ccaatccttc gcgacgatac gtgcggaggc
      961 cgatctctcg cgcattcccg ccgacctcga gaaactcgca gtgcgcgtga tccacgcttg
     1021 cggcatggtc gacgttattg aagacctgcg gttttccgaa gccgcaggcg cggcgggccg
     1081 cacggcactc gcacaaggtg cgccgattct gtgcgacgcc ggcatggtgg cgcaaggcat
     1141 cacgcgtgca cgactgcccg ccaacaacga ggtgatctgc acgctcacgc atcagaacgt
     1201 gcccgcgctc gcgcgcgaac tcggcaatac ccgctcggcc gccgcgctcg aattgtggcg
     1261 gccgtatctg gccggcagcg tggtcgtgat cggcaacgca ccgaccgcgc tctttcattt
     1321 gctcgacatg ctcgatgccg gcgcgccgaa gcctgcgttg atccttggct ttccagtcgg
     1381 ttttgtgggc gcggccgaat cgaaagcgct gcttgccgaa gacagccgcg gcgtgcccta
     1441 cgtcgtggtt cacggtcgac gcggcggcag cgcgatggcg gccgccgctg tgaatgcgct
     1501 ggcgacggag gtcgagtaaa tgaccgtgca aggacgttta ttcggactgg gcgtcggccc
     1561 tggcgacccc gaactcatca cgctgaaagc gctgcgtttg ctgaaggcgg cacccgtggt
     1621 cgcgtatttc gttgcgaagg gtaagaaggg caacgcgttc agcatcatcg aagcgcattt
     1681 gcatgacgcc cagcagcatc tgccgctcgt ttatccggtc acgaccgaag cgctcgagcc
     1741 gccgctttcg tatgaagcga tcatcgccga cttctacgac accgccgcgc agatcgtcgc
     1801 ggatcacctg gacgcgggcc gcgacgtcgc ggtgatttgc gaaggcgacc cgtttttcta
     1861 cggctcgtat atgtatttgc acgaccggct cgcgccccgc ttcgaggccg aagtcgtgcc
     1921 cggcgtgtgc tcgatgctcg gtggcgcggc ggtactcggt gcgccgctgg tgtatcgcaa
     1981 tcagagtttg tcggtgctct ccggcgtctt gcccgaagac gaattgcgcc ggcgtcttgc
     2041 cgatgcggat gctgcggtca tcatgaaact cggccgcaat ttcgataaag tgcggcgcgt
     2101 gctcggcgaa ctgggactcg cgcatcgtgc gctttacgtt gagcgtgcaa cgatgggcaa
     2161 tcaacgcatt gtgccgctcg ccgaggtgga tccgatggct tcgccgtatt tttcgctgct
     2221 cgtcgtgccg ggggaaaaat ggcaaggatg atgccgcctg cgatcgtggt tctcggtgcg
     2281 ggcgcgctgg cgactgcgcg acgtattcag gcgctgtatg caggttgcga ggtgcatgcg
     2341 ttgcgggggc gagtcgaggc tgacgtcgcg tatacggagc tcggcgcgca tttgcgtgaa
     2401 ctgtatgcgc gcggcacgcc gatcgtcgcg ctgtgcgccg ccgggattgt gattcggtgc
     2461 gttgggccgt tgctgtcgaa caagggcgtt gagccgcccg ttctggcggt tgccgaagat
     2521 ggcagcgcgg tcgtgccgtt gctcggcggc cttgcaggcg tcaacgtgat ggcgcgtgag
     2581 attgcggccg cattgggcgt gtcgcctgcg attacgacga gcggagaact gcgcttcggc
     2641 acctgtgtgc tcaatccgcc tgacggctat gcgctcgccg atatcgtcca aggtaagcgc
     2701 ttcgtgtcgg atttgctggc cggagaaagc acgcggattg aaggcgaagc gccgtggctc
     2761 gacgatgcgc aactgccccg ctctgattcg gcacgtctcg cgattcgcgt gacgccgcgc
     2821 gcctcgcgcg gacgtgatga tgagttggtg attcatccgc gaagtgtggt ggcggcggtg
     2881 acggtgggcg gtgctgggac tgatgctggt gctagtgcta gtgctagtgc tggtgctggt
     2941 gctagtgccg ggggcgggac gattttcgct actgccgccg gcccaaccaa ttccagtccc
     3001 gaagcgcaat cgcattcagg cgaacagata gctgctcgcg tgcgtgaggc attggacgca
     3061 cacggtctcg ccgcgctttc tctcgccgca ttactggcat cttctgagca aatggccaac
     3121 ccagcgttcg ctgacgcggc ggcgagcctg aacgtgccgc tgcgttttac tcaaatacgc
     3181 ccggaggacg gtgagccgcc gggcaacctg ctgcatgccg cgctgcgcat cccctacgaa
     3241 acgctgcaca aggacacaaa tacaggcgtt gcgctggcat tagcgccctt ggccatcgac
     3301 gcggacacga ttggccgacc acgcggccgc ttgacggtga tcggcctcgg ccccggcagc
     3361 gccgatctga tggtgcccgc cgctcgaaca gcgttgaacg aagccaccga tatcctcggc
     3421 tacgaaacct acgtgaaaat ggccggcccg tttcgcgcgg atcaacgcgt cgacggcacg
     3481 gacaatcgtg aggaaatgca gcgcgcgcgt catgcgttcg aactcgctag cacaggacga
     3541 tccgtggtga tggtgtcatc gggcgatcct ggcgtgtttg cgatggcggc tgcggtgctc
     3601 gaggcgctcg aagaaggcag ttcgacgaac gctgactggc gcgcggtgca actcgccatc
     3661 gtgccgggca tatccgcggc catggcgacc gcagcccaag ccggcgcacc gttgggccat
     3721 gacttctgca tgctgtcgtt atccgacaat ctgaaaccgt ggaccatcat cgagaagcgt
     3781 ctcaggcatg cggccgaagc agatctggtg atggctttct acaacccgat ctctcgcgca
     3841 cggccatggc aactggacaa ggcactggac attgtgcggg aataccgcgc cgcagcgacg
     3901 aaggtggtgc tcggccgcga catcggcaga ccgggcagca cactgcgaac gaccacgctc
     3961 ggcgaactgc gttcgtcgga tgtggatatg cggacgatgg tgatcgtggg ctcgtcgacg
     4021 acgcgggggt ttgctaatgg catcgacggt ggcgagtggg tttatacgcc gcggtggtat
     4081 gagtgagcac cttgctcgcc cgactcctca ttctcaaccg gtccctacga cggcggaagc
     4141 ctttcaattt gccttctata aattataaaa gtccttcaaa atgggctgaa atcttaattc
     4201 gacgtcgcat tttttagact gagtaagaat tttcaaagcc tggctttctg agttgtaata
     4261 gtaagcatcc caaatatttc gccattcaca cgttttcgga tttctccgat ttcgtcgccc
     4321 ctctgcttcg ccaaaactca gcgactactt acttccgacg cagcgttcgg cgcctctctc
     4381 acaaggtgtg gaccgcagct tcgcagcgaa ggctacgtta ccgaaacgaa gatgatggaa
     4441 ctgaggcgcg ccgctgcgga ttatcagtcg cgtcagcaat cggatgccgc ggagctgatc
     4501 cgctcgcggc agaagcaaac ggatctcgaa ctgaagatcg ccacgctgcg taacgactat
     4561 gtgaaatcgg ctgatagcca gttaaaggac acgacagcaa agattctgca gatcacggaa
     4621 cagttgcgac cggcacagga cgtcgacacg cgcacgcgca tcgtcgcgcc agttgcgggt
     4681 gaagttgtgg gtctgagggt gcacacgatc ggtgcgagca tcgggccgcg tgagccgatt
     4741 ctcgatctgg tgcccactgg ggtgccgtta atcgtcgaag cgaagatcaa gccggacaac
     4801 gtgcgcgaga tcgtgccgaa cagcaaggcc gatgtgcggc tgaccgccta taacccgcgg
     4861 acttctcctg tgctcgaagg cacggtgact tatctgtctg cggacgcact gaacgataag
     4921 gacacgcggc aaccgtttta tctggcgcat atcgaggtct cagccaaggc gatcgcgaag
     4981 gcgaaccggc tggcgaaaga gccgatcgtg ctcgggccgg gattgcgcgc tgaggttttt
     5041 atcaagacgc gatcgagaag cgcgttcgac tatctgtttg agccggtttg ggatgggatt
     5101 cagaggtcta tgcgggactg aaaatggcac gctatatgaa acaca
//
LOCUS       DATASH010000493         2868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3438487,
            whole genome shotgun sequence.
ACCESSION   DATASH010000493 DATASH010000000
VERSION     DATASH010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3438487"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VHV99_13600"
     CDS             <1..292
                     /locus_tag="VHV99_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016346713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636501.1"
                     /translation="TPSNEGTATIGFAAHCDNMLGCEPNPWKAGADFKQFVRGRPSQM
                     GTPGGATAAGNALPPVSPVPPSRPMPQNLPVQPMPPTLQNQPGQSGQDLTPQ"
     gene            1020..1211
                     /locus_tag="VHV99_13605"
     CDS             1020..1211
                     /locus_tag="VHV99_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636502.1"
                     /translation="MHVLPPNLSSWKLALYVIVFLLPGGSFVVLGAAWFENRRTRNTA
                     HPAPAAKTALLCRPKHCDA"
     gene            1486..>2868
                     /locus_tag="VHV99_13610"
     CDS             1486..>2868
                     /locus_tag="VHV99_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008352382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEX3636503.1"
                     /translation="MPFTRIPVFPAKSAIVLALVLTMAGCAVGPNYKRPDTAIPASYK
                     EAPEGWKVAQPSDRVDRGNWWAIYDDPQLNDLETRLNASNQTIAQFAAAYRQSRALVS
                     EARAAYFPTLGLSATGSRAGSSGRSTSTSSSSRGITNNYDLSLDASWEPDLWGTVSRT
                     VGAQRAGEQSAAADLANARLSAQGTLAQTYFQLRSLDAQQKLLDDTVAAYQRTLQLTQ
                     NQYAQGTAARSDVIQAQTQLLSAQASAIDNGVARAQFEHAIAVLVGEPASTFSLASSP
                     LDVTPPDVPLQLPSAILERRPDIASAERKAAQANEQIGVAIAAFFPTLTLSASGGFQS
                     SIFSQLLTAPSRFWTLGPDLAATIFDGGLRAAQTEAARASYDTAVATYRQAVLTAFQD
                     VEDNLASQRILANEVVIQQQAVASAQQALAIVTNQYKSGTVGFINVLTAQTTAFIAEQ
                     KLANIAGERMV"
BASE COUNT          576 a          920 c          823 g          549 t
ORIGIN      
        1 gacgccgagc aacgaaggga cagcgacgat cggtttcgcc gcccattgcg acaacatgct
       61 cggttgcgag ccgaacccgt ggaaggcggg cgcggacttc aagcagttcg tgcgcggcag
      121 gccatcgcag atgggcacgc cgggcggtgc gacggcggcc ggcaacgcat tgcctccggt
      181 ctctcccgtc ccgccatcgc ggccgatgcc gcaaaacctt ccagtacaac cgatgccgcc
      241 tacgctgcag aatcagccag gacaatccgg ccaggatctg acgccgcaat aggtttcatc
      301 gattgttgat tgctgaaaac cgcgctctcg tcagaggcgc ggctttttaa ttatgcggtc
      361 aagcaaaggc ccaattcact ggaatacggc catcaacgac gagaatgtgg cggctacata
      421 ctaattagtg catcgacaga ctggttacta tcgactcgat agctaaaacc cttgcatagg
      481 cattgtttta atccgcgagt gcaatgcggt gtgtcttcgc ctaattgatg tgctgacgga
      541 gactccggca gccgcaaatg cggatcgatg tgtacgacta aaagtgtccg gatgggcgtt
      601 aaacacattc atcgcttcat catccccgcg gccctatgtc gcaccgctgg ctccgggtgg
      661 cacgtggtcc ctccaatgcg cagcggtcct ctccattcag agttgatttt ttccagaagt
      721 ccctgctctg acgggctaaa agccgtgatc aggagccgct atcgctgcaa gcccgacgct
      781 cgcgactgaa aaacaacacc gcaaaaattt gctaaagaac gcatcttgaa aatcgattgc
      841 atagtcaata ttattctaag caacgagtta cgcgctacgg cgccaccccg agaccatgtt
      901 ctgaccgact gaaagcatcg aaccattcga tgcgtaaatg gatttacccg atgaaccgcg
      961 ctgcccaaac caccctcctc ctcggccgac gcttcggcca ctcgatatcc gccgccttga
     1021 tgcacgtcct gccgcccaac ttgagctcat ggaagctggc gctgtacgta atcgtcttct
     1081 tgctgccggg cggatcattt gtcgtactgg gtgccgcatg gttcgaaaac cgccggacgc
     1141 gcaatacagc gcacccggct cccgccgcca agactgcgct gctctgtcgt ccgaaacact
     1201 gcgacgcctg ataccttcgc cgccgttcct ggcgcgtcgt tatgcaactt acgcagcgcc
     1261 tgagcgttgc gtgaattgca caaccgttgc gtaactgctg cgtaatcacc cgtaaccgat
     1321 tccgcacggc gcgtaacacc cggcttcctt tcaaaccgtt accctaacgc ctgcccgacg
     1381 cccgccctac acgtgcgttc agttaccatt tcggaccggt tgcacgaagg cttcgcgccg
     1441 cattgcgttg aagcacgcgc cacctccgac aggaatccag agcagatgcc gttcacccga
     1501 atacccgtct ttcccgccaa gtccgctatc gtcctcgcac tggtactcac catggccggt
     1561 tgcgccgtcg gtccgaacta caaacgtccc gataccgcca ttcccgcatc gtataaagag
     1621 gcgccggagg gctggaaggt tgcgcagcct tcggaccggg tggatcgtgg aaactggtgg
     1681 gcgatttatg acgacccgca gttgaacgac ctcgaaacgc ggctgaacgc gtcgaaccag
     1741 actattgcgc aattcgcggc ggcttatcgg caatcgcggg cactcgtctc ggaagcgcgc
     1801 gcggcgtatt tcccgacctt ggggttatcg gcgacgggct cgcgggcagg ttcctcgggc
     1861 cgctcgacca gcacgtcgtc cagtagtcgt gggatcacca ataactacga tctttccctc
     1921 gatgcatcgt gggaaccgga tttgtggggc accgtcagcc ggacggtcgg cgcgcagcgc
     1981 gcgggtgagc aaagcgccgc agccgatctc gccaacgcac gtctctcggc gcaaggcacg
     2041 ctcgcgcaaa cgtacttcca gttgcgttcg ctggacgcac agcaaaagct cctcgacgac
     2101 accgtcgccg cctatcagcg cacgctgcaa ctcacgcaga accagtacgc gcaaggcacg
     2161 gccgcccgct cggacgtgat ccaggcgcag acgcagttgc tgtcggcaca ggcgtcggct
     2221 attgataacg gcgtcgcccg cgcgcaattc gaacacgcga tcgccgtgct ggtcggcgaa
     2281 ccggcgtcaa cgttctctct cgcgtcatcg ccgctggacg tcacaccgcc ggacgtgccg
     2341 ctgcaactgc cgtctgccat cctcgaacgg cggcccgata ttgcgtcggc cgagcgcaag
     2401 gcggcgcagg cgaacgaaca aatcggcgtg gccattgcgg cgtttttccc cacgctgacg
     2461 ttgtcggcaa gcggtggttt tcaaagctcg atattctcgc aattgctgac cgcgccatcc
     2521 cggttctgga cactcgggcc ggatctcgca gccactatct tcgatggcgg cttgcgggcc
     2581 gcccagaccg aagcggcgcg cgcgtcgtat gacacggccg tggcgacata tcggcaggct
     2641 gtgctgaccg cgttccagga cgtcgaggac aacctggcgt cgcagagaat tctggcgaac
     2701 gaggtcgtga tccagcagca agcggtggcg tcggcgcagc aggcgctggc tattgtgacc
     2761 aatcagtaca agtcgggcac ggtcgggttt atcaacgtgc tcaccgcgca gactacggcg
     2821 ttcatcgccg agcagaagct ggcgaatatc gccggggagc ggatggtg
//
LOCUS       DATASH010000494         1959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2254182,
            whole genome shotgun sequence.
ACCESSION   DATASH010000494 DATASH010000000
VERSION     DATASH010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2254182"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..>1959
                     /gene="secA"
                     /locus_tag="VHV99_13615"
     CDS             47..>1959
                     /gene="secA"
                     /locus_tag="VHV99_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011544506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEX3636504.1"
                     /translation="MTTGFLQKIFGSRNQRLVKQYQKTVTAINALEPQIEQLTDDQLR
                     AKTGEFRQRVASGESLDKLLPEAFAVCREASKRVLKMRHFDVQLIGGMVLHYGKIGEM
                     RTGEGKTLVATLPVYLNALSGRGVHVVTVNDYLAQRDAEWMARLYNFLGLSVGINLSQ
                     MDHGAKQEAYAADITYGTNNEFGFDYLRDNMVYETDARVQRALNFAVVDEVDSILIDE
                     ARTPLIISGQAEDHTELYVRMNALPPLLERQIGEEKADGTGVEKPGDYTLDEKGRQVF
                     LTESGHEKAERLLAEWGLIGEGESLYAPQNITLMHHVYAALRAHTLFFKDQHYVVQNG
                     EVVIVDEFTGRLMSGRRWSDGLHQAVEAKEHVKIQSENQTLASITFQNYFRMYAKLSG
                     MTGTADTEAYEFNEIYGLETVVIPTNRPPKRIDKQDQIYKTAKERYDAVIRDIRDCYE
                     RGQPVLVGTTSIENSELLSHLLKQAGLPHEVLNAKQHAREAEIVAEAGRPKRVTIATN
                     MAGRGTDIVLGGNAEKQASFLEQDETLSDEEKQRRIQKLHDEWQALHDQVKAAGGLHI
                     IGTERHESRRIDNQLRGRAGRQGDQGSSRFYLSLEDPLLRIFAGDRVRAIMDRLKMPE
                     GEAIEAGIVSRSI"
BASE COUNT          439 a          597 c          606 g          317 t
ORIGIN      
        1 ccgcacgccg cgaaccagcc gaagccgcga cgcagacacc gatccgatga ccaccggttt
       61 tcttcagaag atttttggca gccgcaacca gcggctagtc aagcaatatc aaaagaccgt
      121 cacggcgatc aatgcgctcg aaccgcagat cgagcaattg acggacgatc aactgcgcgc
      181 caaaacgggt gaattccgcc agcgcgtcgc gagtggcgag tcgctcgaca agctcctgcc
      241 ggaagccttc gcggtgtgtc gcgaggctag caagcgggtg ctgaaaatgc gccacttcga
      301 cgtgcagctg atcggcggca tggtcctgca ttacggcaag atcggcgaaa tgcgcaccgg
      361 cgaaggcaag acgctcgtcg ccacgctgcc ggtgtacctg aatgcgttgt cgggccgcgg
      421 cgtgcacgtc gtcacggtca acgactacct ggcacaacgc gacgccgaat ggatggcgcg
      481 tctgtacaac ttcctcggtt tgtcggtcgg catcaatctg tcgcagatgg atcatggcgc
      541 gaagcaggaa gcgtacgccg ctgacatcac gtacggcacc aacaacgaat tcggcttcga
      601 ctacttgcgc gacaacatgg tctacgagac cgacgcgcgc gtgcagcgag ccctgaattt
      661 cgcggtggtc gacgaggtcg actcgatcct gatcgacgaa gcccgtacgc cgctgatcat
      721 ttccggccag gccgaagatc acaccgaact ctacgtgcgc atgaatgcgc tgccgccgct
      781 gctcgaacgt cagatcggcg aagagaaagc ggatggcacc ggcgtcgaaa aaccaggcga
      841 ctacacgctg gacgagaaag gccgccaggt cttcctgacg gaatcgggtc acgaaaaggc
      901 cgagcgcctg ctcgccgaat ggggcctgat cggcgagggc gaaagcctgt atgcgccgca
      961 gaacatcaca ctgatgcacc acgtgtacgc cgcactgcgc gcgcacacgc tgttcttcaa
     1021 ggatcagcat tacgtggtgc agaacggcga agtcgtgatc gtcgacgaat tcaccggccg
     1081 gctgatgtcg ggccgccgct ggtcggatgg cttgcaccag gccgtcgagg ccaaggaaca
     1141 cgtcaagatc cagagcgaga accagacgct cgcgtcgatc acgttccaga actacttccg
     1201 catgtacgcc aagctgtccg gcatgaccgg cacggccgat accgaagcgt acgaattcaa
     1261 tgagatctac ggtctcgaaa cggtcgtgat cccgaccaac cgtccgccga agcggatcga
     1321 caagcaggat cagatctaca agaccgccaa ggaacgctat gacgcggtga tccgcgacat
     1381 ccgcgattgc tacgagcgcg gtcagccggt gctggtcggc accacgtcga tcgaaaactc
     1441 ggaactgctg tcgcatctgc tgaagcaggc cggtttgccg cacgaagtgc tcaacgccaa
     1501 gcagcacgca cgtgaagctg agatcgtcgc cgaagcgggc cgtccgaagc gcgtcacgat
     1561 cgctaccaac atggcgggtc gcggtactga catcgtgctc ggcggcaacg cggaaaagca
     1621 ggcttcgttc ctcgaacagg atgaaacgct ttctgacgaa gagaagcaac gccgtattca
     1681 gaagctgcac gacgaatggc aagcgttgca cgatcaggtg aaggccgcgg gcggtctgca
     1741 catcatcggc accgaacgtc acgaatcgcg ccggatcgac aaccagttgc gcggccgtgc
     1801 gggccgtcag ggcgaccagg gttcgtcgcg cttctatctg tcgctggaag atccgctgct
     1861 gcgcattttc gcgggcgatc gcgtgcgcgc gatcatggac cgcctgaaga tgccggaagg
     1921 cgaggcgatc gaggcgggca tcgtatcgcg ttcaatcga
//
LOCUS       DATASH010000495        10058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4430795,
            whole genome shotgun sequence.
ACCESSION   DATASH010000495 DATASH010000000
VERSION     DATASH010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 10058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10058
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4430795"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            695..1687
                     /locus_tag="VHV99_13620"
     CDS             695..1687
                     /locus_tag="VHV99_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX3636505.1"
                     /translation="MNKKFASSRVSMIVAASVLAFSTVSAHARVFRVADVHSDSFPTN
                     MAVKYMGDEISKATGGKDSVKVFGNSALGSENDTIDQVRIGALDMARANGAAFNEIVP
                     ESMIPSLPFLFRDIDQFRKVMYGPEGQKILDAFTAKGMIALTFYESGARSIYAKKAIH
                     SPADMKGVKVRVQPSDLMVDEIKAMGGTPTPMPFAEVYTGLKTGLVDAAENNIPSYEE
                     TKHFEVAQVYSETDHSMTPEVLVFSKKVWDTLTPQEQTIIKKAAADSVPYYQKLWTAR
                     EADAAKTVTKGGATIIPASQIDRPAFVKAMQPVWAKYEKTPAMKQIVDEIQAVK"
     gene            1826..2305
                     /locus_tag="VHV99_13625"
     CDS             1826..2305
                     /locus_tag="VHV99_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX3636506.1"
                     /translation="MRFMKRPNDFLFRALVVVAAVSLAVLCVLVIYSVVMRYVFSDAP
                     DFVEPIALLLVIVIAMFGAALKVREGGHIGLDSFVKQLSPKAQVVAHAFQHLCLIVLA
                     GAIFFGCLQMAETTMNDKIPILGLPEALRYLIPIIASVCIALFSFENLLGLFARTQK"
     gene            2313..3665
                     /locus_tag="VHV99_13630"
     CDS             2313..3665
                     /locus_tag="VHV99_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX3636507.1"
                     /translation="MELAILSVSFLVFLVLGVPVSFALGLSCVLTYLHEGLPAATAMQ
                     SMISGMNAFSFLAVPFFIFSGELMLHGGIADRILRFAQATVGHFRGGLGMANVVACTL
                     FGGVSGSPTADTSAMGGVVIPMMKREGYSAAYAVNVTTHSSLAGALMPTSTNMIIYAF
                     AAQGITGTLNGQQLSGVSIGDLLFSGLLPVLWVMGFVLIAAYWQAVKYGYPRSADGST
                     ELQRFPGWLAVARTFLGALPGLMVIAIILVCVSRGIATATEAAAIAVAYSLVLTIVVY
                     RTMTMKKLAHALGKAAKTTGVVLLLIGVSNMLRFQMAYLEIPDAIEHMLDGATSLPWL
                     MLLYINVIQIFLGTFVDMAAHILITTPLFLPMAMHAGVGPVQFGIMILLNCSLGLVHP
                     PIGSVQFIGCAIGNVSIGETTKVAWPYYLAIFSAINIVTYVPMFSTWLPSLINGHPVF
                     "
     gene            3793..4998
                     /locus_tag="VHV99_13635"
     CDS             3793..4998
                     /locus_tag="VHV99_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015603285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX3636508.1"
                     /translation="MKRYKEAAVAVAGLAAMFATGGAFAQSSVTLYGVLDTGVGYLSS
                     QAPTTGATSGGKSVTKLIEGVWGGERFGFKGSEDLGGGTKAIFQLEEGYNVDNGAQSK
                     SGLMFSRASWVGLTNNTYGTFTAGRQYTPYYNMLAQYGPTPWLTGAFGAHPGDLDALD
                     TDYRVNNALVYTSPSFGGLKVSGMYALGGVAGAFNSGASWSVGAQYLAGAAGIGVGFA
                     RFNNATSGGGAWSANSTAYSGTGEQGVSSITNGYQNAAAQQRFAVTGGYQFNSQWDVS
                     ASYSNVQYIAGPNSGFATTAIFNTGGAVLHYKPTVAWDLAAGYSYTRATQANGVQDAA
                     SYQQFNLTQLYNLSKRTRIYVLEAYQRANGQTINGGKVVTATAAIGEQSAASSRSQFG
                     ATVGINHQF"
     gene            complement(5327..5444)
                     /locus_tag="VHV99_13640"
                     /pseudo
     CDS             complement(5327..5444)
                     /locus_tag="VHV99_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433112.1"
                     /note="4-OT; member of subfamily 5; forms a dimer; the
                     function in the Escherichia coli cell is unknown;
                     frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
     gene            5466..6408
                     /locus_tag="VHV99_13645"
                     /pseudo
     CDS             5466..6408
                     /locus_tag="VHV99_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491517.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
     gene            6463..7662
                     /locus_tag="VHV99_13650"
     CDS             6463..7662
                     /locus_tag="VHV99_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015603285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX3636509.1"
                     /translation="MKKAIATSALGLVVLGAHAQSSVTLYGIVDTGIGYQSSSTSLGS
                     TTGGRSVVKMINGIWAGSRFGLKGSEDLGGGTKAIFQLEEGFNSANGAQSVSGLMFSR
                     QAYVGVANSTFGTLTAGRQYTSYYTLLSPYSPTTWLTGAYGAHPGDIDSLDTLYRANN
                     SLVYTSPNMYGLTVSGSYSLGGVPGSTNAGSTWSAALQYLNGPFGIAAGFQRINNSTP
                     GGGAWGADSTTSNAGAQPGVSGINNGYQTAQAQQRIAVTGGYAFSSQWDVSFAYSNVQ
                     YIPGVNSKFFSQATFNTAGAVLHFKPLTALDLGAGYSYTRATESNGISSAATYQQFNL
                     SQYYSLSKRTGLYALEAYQRAGGQTIGTNGRSIIEATADIGDGQNTAPSSSRSQVAVG
                     VGIIHRF"
     gene            complement(7946..8137)
                     /locus_tag="VHV99_13655"
                     /pseudo
     CDS             complement(7946..8137)
                     /locus_tag="VHV99_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006052330.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
     gene            complement(8194..8970)
                     /locus_tag="VHV99_13660"
     CDS             complement(8194..8970)
                     /locus_tag="VHV99_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008347659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX3636510.1"
                     /translation="MSFTHAPSGRVAEAGPRSKAEQQTRVDLAAAYRLAALNDWDDLI
                     YTHISASVPGEPGHFLINPFGLAFDEVCASNLVKIDIEGNIVGTSEHPVNATGFALHA
                     AVHAARTDAVCVMHLHNTAGVAVSAQPAGLLPASQHALRFYGHLAYHEYEGLAFTPTE
                     GERLVAHLADKPAMLLRNHGTLTTGRTVAEAYVLMATLIKACEIQLQAQACGSALVVP
                     NDAVATRTADQLYDGGAVEGALEWPALLRKLDRLDRTYRD"
     gene            complement(9011..9958)
                     /locus_tag="VHV99_13665"
     CDS             complement(9011..9958)
                     /locus_tag="VHV99_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015204 - urea transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071918 - urea transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HEX3636511.1"
                     /translation="MHAAATEAPSAALRTLLRSLGQIVLQPNAFAGACLLAAWLLYDP
                     RLACAALMGAVAANVSAVLAGYREDDTRAGLHGFNGALAGLAAFSFIADNATAAAVAI
                     LAATGTAWLLEPWSRRLRAHGLGFFSSPSLIVTWLWLPLVTSGTHAASLPAVHTVSAA
                     QLSDGLLAGFAQTGFASGALPGLLVLTGIAAASRRHALWALIGAGLASAAQLLLGASA
                     SSFDAGLLGFNGALTAIALADFGIAATLGGIALSVVLQMFATYYAWPAMTAPFVLATW
                     SMQWLTGRCMHGAAVAEPAERADEAERPVASPGSVGRVG"
BASE COUNT         1890 a         2907 c         3184 g         2077 t
ORIGIN      
        1 cctggcgtga gcggcctgcc cgagcccgaa tacgcgggcg aactctaatc gataagcaca
       61 tcaggcgcgc tggggtgggt catacccggc gcgattcact ccatggccgc ttgattcatc
      121 actcgtcgta caacacttct ctgcgttgtg gtgtagggac cacatttcga gacgctcgta
      181 tgcaggaaaa acagcgcgac cggacacctg ctcgaccact cggcgacgtt gcttgccgct
      241 gtggtaagcc tttgcgccat gccacgccag tcactcgtac cgatacatcg agcctgcggg
      301 taaatactgg ctgtacaaca tagaacgtgt tttatagact tcatatgtcg tcgtacaacg
      361 tataagtcaa caacgaattc ctttccgtga atttcgagat tgctggactt aaggcgaggt
      421 tcttgatgcg catattccct gcgcatcaag actgatcccg atgtagtcga agtagagaag
      481 ttgagtcgtc ccagaggagt gctgaggatc tgtgccgggg acattgcatg ggaccagcac
      541 gttagtactc tcgccgcccg aaagggcggt aatcgtacgg cagagaaaaa aggcgcggca
      601 tttttttgaa gccgggagac gcattaaaaa accatgtcgc agcatccgtc agcggccctc
      661 cgcaatcggt tgctactttt caaggaatgt cacgatgaac aagaagtttg cctcttcccg
      721 tgtttcgatg attgtcgcgg cctccgtgct ggcgttcagc accgtgtcgg cacacgcccg
      781 cgtgtttcgt gtggcggacg tgcatagcga cagctttccg accaacatgg ccgtgaagta
      841 catgggcgac gaaatcagca aggcaaccgg tggcaaggat tccgtaaagg tcttcggcaa
      901 cagcgcgctg ggttccgaaa acgacacgat cgatcaggtg cgtatcggcg cactggacat
      961 ggcgcgcgcc aacggtgctg cgttcaacga gatcgttcct gaatcgatga tcccgtcgct
     1021 gccgttcctg tttcgcgaca tcgatcaatt ccgcaaggtc atgtacggtc cggaaggcca
     1081 gaagattctc gacgccttta cggcgaaggg catgatcgcg ctcacgttct atgagagcgg
     1141 cgcgcgctcg atctatgcga agaaggcgat ccattcgccg gccgacatga agggcgtcaa
     1201 ggtgcgcgtg cagccttcgg atctgatggt cgacgaaatc aaggcgatgg gcggcacgcc
     1261 gacgccgatg ccgttcgccg aagtctatac gggcctgaag acgggtctgg tcgatgccgc
     1321 ggaaaacaac ataccgtcgt acgaagagac caagcacttc gaagtcgccc aggtctattc
     1381 cgaaaccgat cattcaatga cgccggaagt cctggtgttc tccaagaagg tgtgggacac
     1441 gctgacgccg caagagcaga ccattatcaa aaaggctgcg gctgactcgg tgccttacta
     1501 ccagaagctg tggaccgcgc gcgaagccga cgcggcaaag acggttacca agggcggcgc
     1561 gacgatcatc ccggcctcgc agatcgaccg tccggctttc gtgaaggcga tgcaaccggt
     1621 ctgggcgaag tacgagaaga cgccggcaat gaagcagatc gtcgacgaaa tccaggcagt
     1681 caagtaattc ggcggtagag cggtaaggaa aggccttgca gcaggcgctg cgggtctttc
     1741 gcgccgtgcg gtctcttgaa ccggcgatag tcagcaggat tcctgaagat taaaaataag
     1801 ttcgttgtat gcaaggatgg gttcaatgcg attcatgaag cgccccaatg attttctctt
     1861 ccgcgcgctg gtggtcgtgg cggcggtgag tcttgccgtg ctatgcgtgt tggtgattta
     1921 cagcgtcgtg atgcgctatg tcttcagcga tgcaccggat ttcgtcgagc ccattgccct
     1981 tttgctggtg attgtgattg ccatgttcgg cgccgcgctc aaggttcgtg agggcggcca
     2041 tatcgggctc gattcattcg tcaagcaatt gtcgccgaaa gctcaagtgg tcgcgcatgc
     2101 gttccagcat ctctgcctga ttgtgctggc tggggcgatc ttcttcggct gcctgcagat
     2161 ggcggagacg accatgaacg acaagattcc gattctgggt ctgccggaag cacttcgtta
     2221 cctcattccg atcattgcca gtgtctgcat cgctctgttc tcattcgaga acttgctggg
     2281 gcttttcgca cggacacaaa aatagaagaa tcatggaact tgccatcctc tcagttagct
     2341 tcctcgtttt cctcgtactc ggggttcctg tttcgttcgc gctggggctc tcgtgcgtcc
     2401 tgacctatct gcatgaagga ttgccggcgg ccaccgccat gcagtcgatg atttcgggta
     2461 tgaacgcgtt ttcgttcctc gcggtcccgt tcttcatatt ctccggcgag ttgatgttgc
     2521 atggcggtat cgccgaccgc atcctgcgtt tcgcgcaggc caccgtgggt cattttcgcg
     2581 gtgggctcgg catggccaac gtggtcgcgt gcaccttgtt cggcggcgtg tccggttcgc
     2641 cgacggcaga cacctccgcc atgggtggcg tcgtgatccc gatgatgaag cgcgagggct
     2701 atagcgcggc ctacgcggtc aacgtgacga ctcattcgtc attggccggt gcgctgatgc
     2761 ctacgtcgac gaacatgatc atttacgcct tcgcggccca aggcatcacc gggacgttga
     2821 acggtcagca gctgagcggt gtgtcgatcg gcgatctgtt gttttctggt ctgttgccgg
     2881 ttctgtgggt gatgggtttc gtgctcattg ctgcgtactg gcaagcggtc aagtacggtt
     2941 atccgcgcag tgctgacggt tcgaccgaat tgcagcgctt tcccggctgg ctcgcggtgg
     3001 cccgcacgtt cctcggcgca ttgcccggcc tgatggtgat tgcgatcatc ctggtctgcg
     3061 tgtcgagagg gattgccaca gcgacggaag cagcagccat tgccgtggcc tattcgctgg
     3121 tgttgaccat cgtcgtctat cgcacgatga cgatgaagaa gctcgctcat gcgcttggga
     3181 aggcggctaa gaccaccggc gtcgtactgc tgctgatcgg cgtgtcgaac atgctgcgtt
     3241 tccagatggc gtatctggag attcccgacg cgatcgaaca catgcttgac ggcgcgacga
     3301 gcctgccctg gctgatgctg ctgtacatca acgtcattca gattttcctc ggcacgttcg
     3361 tcgacatggc cgcgcacatc ctgatcacca cgccattgtt cctgcccatg gcgatgcacg
     3421 ccggcgttgg cccggtgcag ttcgggatca tgattctgct gaattgttca ttgggcctcg
     3481 tgcatccgcc gatcggttcg gtgcagttca ttggttgcgc gatcggcaac gtttcaatcg
     3541 gcgaaaccac caaggtggcg tggccgtact acctggcgat cttcagcgcg atcaatattg
     3601 tgacgtacgt gccgatgttt tcgacgtggt tgccgagtct gatcaatggc cacccggtgt
     3661 tttgaacgtt tgatcaagcg ttagcgctgg tgaggcactg ccaggcatga agaacgggtc
     3721 agcgtgatca gcatggtgac ccgcgtgtca ctgtttcaaa gctaattatt aacaacaaga
     3781 agggacgatg tgatgaaacg ttataaagaa gccgccgtag ccgtcgccgg cctggcagcc
     3841 atgtttgcta cgggcggggc ctttgcgcag agcagcgtga cgctgtatgg ggtgctggat
     3901 accggggtgg gttatctgag cagccaggcg cccacgactg gcgcgacctc gggcgggaaa
     3961 tcggtgacca agttgatcga aggggtgtgg ggcggcgagc gcttcgggtt caaggggagt
     4021 gaggacctgg gcggcggcac caaggcgatc ttccagcttg aagagggtta caacgtcgac
     4081 aacggcgcac agagcaagtc cggtctgatg ttcagccgtg cttcctgggt cggcctgacc
     4141 aataacacct acggtacgtt cacggccggt cgccagtaca cgccgtacta caacatgctc
     4201 gcgcagtatg gcccgacccc gtggctgacc ggcgccttcg gtgcccatcc gggcgacctt
     4261 gacgcgcttg atacggacta ccgcgtcaac aatgcactcg tctacacctc gccttcgttc
     4321 ggcggtctga aggtgagcgg catgtacgcg ctgggcggcg tggctggtgc cttcaactcc
     4381 ggtgcgtcgt ggagcgtcgg tgcgcagtat ctggcgggtg ccgccggtat tggcgtcggc
     4441 ttcgcgcgtt ttaacaacgc gacgagcggc ggtggcgcct ggagcgccaa ctcgacggcc
     4501 tattccggca ccggcgagca gggtgtgtcg agcatcacga acggctatca gaacgcagcg
     4561 gcgcagcagc gctttgctgt gacgggcggc tatcagttca actcgcagtg ggacgtctcg
     4621 gcgtcgtatt cgaacgtgca atacatcgcc ggccccaact cgggcttcgc gacgaccgcg
     4681 atcttcaaca cgggcggcgc ggtactgcat tacaagccga ctgtggcgtg ggacctggct
     4741 gccggctata gctacacgcg tgcgacgcag gccaacggcg tgcaggacgc ggcgagctat
     4801 cagcagttca acctgaccca gctctataac ctctccaagc gcaccaggat ctatgtgctg
     4861 gaagcgtatc agcgagccaa cggccagacg atcaacggcg gcaaggtcgt cactgcgacc
     4921 gcggctatcg gcgagcaatc tgcggcgtcg agccgcagcc agttcggcgc gaccgtgggt
     4981 atcaatcacc agttctgatg cagtagcagc gtgagggggc gccgggtaag gcccgacgcc
     5041 gccaccgatg cagtaattgt cgctgtctga ttgttttgcg gtgaaggctg gcgctgcgta
     5101 gcggcgccag cctttatcgc gtccgcaccg ccgggtatcc tcgttaccca ttctctccca
     5161 cggtttgagc gccatccctt catgcggtag cggcgcccgg ccttggtgcg tcgcgcacgt
     5221 cgtccggctg aagatgaagc cgacggcggc gttttcagcg cccaggatcc aaatcccacc
     5281 agataactct cgaacgccgc ccgacgctca agcggccgga tcggaccaaa cgtccagccg
     5341 tcgcccattt tttcgctttt gacgccagtg agaacgatgt ccaccgagtc cggttccacg
     5401 ccgagtgatt cacaggtcgc tttggtgatg gcttcgacga accggcacgt ctgccatggg
     5461 gggcggtcat tccgctgatg aaacgcgaag gttacagcgc ggcctaatgc ggtcaatgtg
     5521 acgacccatt cgtcgctggc gggcgcgctg atgcccacgt cgacgaacat gatcatctac
     5581 gcttcgcagt ccacggcatc accggcacgc tgaacggtca tcagatgagc gtcgtgtcgg
     5641 tcggcgatct gttgttttct ggtctgttgc cggttctgtg ggtgatgggt ttcgtgctca
     5701 ttgctgcgca ctggcaggtc aagaatggct atccgcgccg tgctgacggc ttgagcgaat
     5761 ggcagcgctt tgccggctgg ttcgcggtgg cccgcacgtt cttcggcgca ttgcccgatt
     5821 cgatggtgat cgcgatcatc ctggtctgcg gggccaaggg cattgccagc gcgacggaag
     5881 cggcggccat ttgccgtggc ctgatcgctg gtgttgacca tcgtcgtcca tcgcgcgatg
     5941 acgatgaaga agctctctca tgtgttgtcc agggcggcca ggaccgccgg cgtggtgctg
     6001 ctgctgatcg gcgtgtcgaa catgcagctt tttcagatgg cgtatctgga gatccccgac
     6061 gctatcgaac acatgctcga cggtgcaaca aggctgccgt ggttgatgct gctttacatc
     6121 gacatcatcc agatcttcct cggtacgttt gtcgacatag cggcgcacat cctgatcacc
     6181 acgacgttgt ccctgcccgt ggcgatgcat gccggcgttg gtcccgcgca gttcatcggc
     6241 tgcgcaatcg gcaatgtgtc gataggtgaa accaccaagg tggcgtggcc gtattacctg
     6301 gctatcttca gcgccattgg tatcgtgaca tacgtaccta tgttttcgac gcgattgccg
     6361 agtctatcaa tggcccccaa atgttttgaa caccttatta aaagttgacg ccggtgtggc
     6421 actgcctggc ataaaaaata aattttttac agaggagcac aaatgaaaaa ggcaatcgca
     6481 acttcggccc ttgggttggt cgtcctcggc gcgcacgcgc aatccagcgt gacgctgtac
     6541 ggtatcgtcg atacggggat cggctatcaa agcagctcga cgtcgctcgg ttcgacgacc
     6601 ggcgggcgtt cggtcgtcaa gatgatcaac ggcatctggg cgggtagccg cttcggcctg
     6661 aagggcagtg aggacctggg cggcggcaca aaggcgatct tccagttgga agaaggcttt
     6721 aacagtgcaa acggcgcgca gagcgtgagc ggtctgatgt tcagccgtca ggcttacgtc
     6781 ggtgtggcca attccacctt cggtacgttg acggccggcc gccagtacac gtcgtactac
     6841 acgctgctgt cgccctatag cccgaccacg tggctgaccg gtgcttacgg cgcgcacccg
     6901 ggcgatatcg attcgctgga tacgctctat cgtgccaaca actcgctggt gtacacgtcg
     6961 ccgaacatgt acggcctgac ggtgagcggt tcgtattcgc tgggtggcgt accgggcagc
     7021 accaatgcgg gttcgacgtg gagcgccgcg cttcagtacc tgaacggtcc gttcggtatt
     7081 gcggcaggct tccagcgcat caacaactcg acgcctggtg gcggtgcctg gggcgctgac
     7141 tccacgacgt cgaatgcggg cgcgcagcca ggtgtgtcgg gcatcaataa cggctaccag
     7201 acggcgcagg cgcagcagcg cattgcggtg acgggcggct acgcattcag ttcgcaatgg
     7261 gatgtctcgt tcgcgtactc gaacgtgcaa tacattccgg gtgtgaactc gaagttcttc
     7321 agccaggcga ccttcaacac ggccggcgcg gtcctgcact tcaagccgct gacggcgctg
     7381 gatctgggcg cgggttatag ctacacgcgt gccacggaat cgaacggtat ttcgagtgcg
     7441 gcgacctatc agcagttcaa cctctcgcag tactacagcc tgtccaagcg cacgggtctg
     7501 tacgcgctgg aagcctatca gcgtgcgggt ggccagacga tcggaacgaa cggccggagc
     7561 atcatcgaag caacggccga tatcggcgat ggtcagaaca ccgcgccgtc ttcgtcgcgc
     7621 agccaggttg ctgtcggcgt gggcatcatc cacaggttct aatcgtgtag tgaagtggcg
     7681 gcgtcaggag acaccgccgc ggcgacgcgt ttcagatgcg cgtcgctttg cctggaagta
     7741 ttcgctgcgc ccgcaacgcg cgcggcattg tcgcaacaca cggacttcga tccggtcgtg
     7801 ccgcgatccg tcccgcgcta ctgcccgccg atggacctgg tggcggtttt ttcagcgcac
     7861 aggatgcaaa tctgtggtga caatcggaca gggatccgtg gtgacatcgg acaaggttta
     7921 gttgttggcc gatatctaca cgctctcacg catccgcttc ggaccacagg cgccctcccg
     7981 ttgcccagtt ttcgcgtttg acgtcggtga gaatgatgtc cactgagttc ggctcgacgc
     8041 cgagcgattc gcaggtcgct ttggtgatgg cttcgacgaa ctggcgtttc tgttacggcg
     8101 agcggccttc gaagagttcg atatgaaacg tgggcattgc gtgactccgt gatgaacggc
     8161 gttgaaaaaa gacgtgaaaa agcgaattca aatttaatct cggtacgtgc gatcgagccg
     8221 gtcgagcttg cgtagcaaag cgggccattc gagcgcgcct tcgacggcac caccgtcgta
     8281 cagctggtcg gcggtgcggg tggctaccgc gtcattgggt acgacgagcg cgctgccgca
     8341 tgcttgcgcc tgcaactgga tttcgcaggc cttgatcagc gtcgccatca atacataggc
     8401 ttcagcgacg gtgcggccgg tggtcagcgt gccgtggttg cgcagcagca tggcgggttt
     8461 gtcggcgaga tgcgcgacga ggcgttcgcc ctcggttggc gtaaacgcaa ggccttcgta
     8521 ttcgtgatac gcgaggtgcc cgtaaaagcg cagtgcgtgt tgcgacgccg gcagtaatcc
     8581 ggccggttga gccgaaaccg ccacgcccgc cgtattgtgc aagtgcatca cgcagacggc
     8641 gtcggtgcgg gccgcatgca cggcggcgtg cagcgcgaaa ccggtggcgt tgaccggatg
     8701 ctcgctcgtg ccgacgatgt tgccttcgat atcgatcttc acgaggttcg acgcgcacac
     8761 ttcgtcgaac gcgaggccga acggattgat cagaaagtgg ccgggttcgc cgggaacact
     8821 ggctgagata tgcgtgtaga tcagatcgtc ccagtcgttg agcgccgcga ggcgataggc
     8881 ggcggccaga tcgacgcggg tctgctgttc ggctttggat ctcggccccg cctcggcgac
     8941 gcggccgctg ggcgcgtggg tgaatgacat gtctgtctcc tgagattgct gggttggctg
     9001 acgctgcggc ttaacccacg cgtccgaccg accccggcga cgcgacgggc cgttcggctt
     9061 cgtccgcgcg ctccgccggt tcggcgactg ccgcgccgtg catacagcgg ccagtgagcc
     9121 actgcatgct ccacgtcgcg aggacgaacg gcgcagtcat ggccggccac gcataataag
     9181 tagcgaacat ttgcaacacg accgaaagcg caataccgcc gagcgttgcc gcgatgccga
     9241 aatcggccag cgcgatcgcg gtcagcgcgc cgttgaatcc taacaggccc gcgtcgaacg
     9301 agcttgcact cgcgccgagc agcagctgcg ctgcgctcgc gaggccggcg ccgatcaagg
     9361 cccaaagggc gtgccggcgc gatgccgcgg caatgccggt caacacgagc aaacccggca
     9421 acgcgccgga cgcaaagccg gtttgcgcga aaccggcgag taagccgtcg ctcaattgcg
     9481 ccgcgctgac cgtatgcacg gcgggcaaac tcgctgcgtg agttccgctg gtcacaagtg
     9541 gaagccaaag ccacgtgacg atcagagaag ggctcgaaaa aaagccgagc ccgtgtgcgc
     9601 gcaggcggcg cgaccatggc tcaagcagcc atgccgtgcc tgtcgcggcc agaatcgcca
     9661 cggcagcggc cgtcgcattg tcggcgatga agctgaacgc ggcgagaccg gcgagcgcgc
     9721 cgttaaaacc gtgcagccca gcgcgcgtgt cgtcttcgcg atagccggcc aataccgcgc
     9781 tcacgttggc cgcgaccgcg cccatcagcg ccgcgcaggc gaggcgtgga tcgtatagca
     9841 gccacgcggc gagcaggcac gcgccggcaa acgcgttcgg ctgcagcacg atctggccga
     9901 gactgcgcag caaggtgcgc aaggcggcgg atggtgcttc ggttgcggcg gcgtgcatgg
     9961 ctttgaaggc ggtgaattcg aggtgacgcg aacttgccgc gtgcgaatca ggcgcacgcg
    10021 gcgggtcagg tttcaggttg agtgtgaacc gcgagcat
//
LOCUS       DATASH010000496         7604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3244147,
            whole genome shotgun sequence.
ACCESSION   DATASH010000496 DATASH010000000
VERSION     DATASH010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7604
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3244147"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VHV99_13670"
     CDS             <1..487
                     /locus_tag="VHV99_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1439 domain-containing protein"
                     /protein_id="HEX3636512.1"
                     /translation="FPFIPSHYTFSQQQVQDAVQRKFPYQRTVSQVFDVALTHPVVGL
                     LPDANRVSVKLDARLVSPFMPQPVDGVFTLSSELAYDAASKSVVLKSPNVDNVSVSGQ
                     AQAYTQQINAAAALLATQLLTNYPIYTFKPEQLQFAGVNYEPGTITILTNGIRVQIVE
                     K"
     gene            571..1407
                     /locus_tag="VHV99_13675"
     CDS             571..1407
                     /locus_tag="VHV99_13675"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018434504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX3636513.1"
                     /translation="MDWLLACKALILGVVEGLTEFLPVSSTGHLIVAGSLLNFTDEHA
                     KTFDVVIQLGAILAVCWEFRRRIGSVVVGLPSRPDARRFTLNVIIATIPAIVLGLLFE
                     KAIKAALFSPVPVAFALVAGGVVILWAEARQRARGDAAARIQNVDDLGPLDALKVGLA
                     QCFALIPGMSRSGSTIIGGMLFGLERRVATEFSFFLAIPIIFGATAYELYKDWHLLSA
                     DALGSFALGFVAAFISAFACVRWLLRYIAAHDFTVFAWYRIGFGLLILLVGYSGALSW
                     AD"
     gene            complement(1505..2299)
                     /gene="trmB"
                     /locus_tag="VHV99_13680"
     CDS             complement(1505..2299)
                     /gene="trmB"
                     /locus_tag="VHV99_13680"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEX3636514.1"
                     /translation="MIHDPNDAGLPDGLPPTSPDTEGQPANANDDNDALQEAGLHRRR
                     IRSFVTRAGRVSTGQRRAMDELGPRFVVPFTPQQPDWNSVFGRATPRVLEIGFGMGAT
                     TAEIASHRPDDDFLGVEVHEPGVGALLKLMGEQGLSNIRIIQHDAVEVLEQMIAPDSL
                     DGVHIFFPDPWHKARHHKRRLIQPKFVALLVSRLKPGAYLHCATDWQNYAEQMLEVLG
                     ADPALENTADGYAPRPEYRPVTKFERRGLRLGHGVWDLVFRKRGAA"
     gene            complement(2793..2866)
                     /locus_tag="VHV99_13685"
     tRNA            complement(2793..2866)
                     /locus_tag="VHV99_13685"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2832..2834),aa:Gly,seq:ccc)
     gene            complement(2951..3514)
                     /locus_tag="VHV99_13690"
     CDS             complement(2951..3514)
                     /locus_tag="VHV99_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010116661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEX3636515.1"
                     /translation="MFGDIARFLLNTVFTLFGAALLLRAWLQVVRLPPYNPVSNAVLQ
                     VTNWIVLPLRHILHSTRNIDWASLVAALLAAIVYVVLMVALTGADPLTLAPTLLIVAV
                     LTLIKWALNLIIWMTILMALLSWLNPRSSAMPILYQLTAPFLNPLRRIVPQLGGIDLS
                     PILLFVIVQVLLMVVTRAAVQLTYFVI"
     gene            complement(3733..4053)
                     /locus_tag="VHV99_13695"
     CDS             complement(3733..4053)
                     /locus_tag="VHV99_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HEX3636516.1"
                     /translation="MIKVSILYPYRENGHFDVEYYCATHMPLAAKLFGPSLKGWSVDV
                     GINAGPPGTPPPYVAAGHFLFDSADDFYKVFKPASEQLLADIPNYTDGGNGAILISEI
                     KVSV"
     gene            complement(4131..5069)
                     /locus_tag="VHV99_13700"
     CDS             complement(4131..5069)
                     /locus_tag="VHV99_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HEX3636517.1"
                     /translation="MSHPSLPRLGFIGAGRLARCLALSFSRAGYPVTAVASRTPASAS
                     RLASQIEHCAALDNQQQVVDAADIVFLAVPDDSIGTTAHTLRFAADAVSGPHGKALVH
                     CSGASPVELLAPARAQGAAIGGFHPLYLFGGDLADLERIAGCSVTIEADGTLKDALTA
                     LAVALRCHPLSIPAGGRMLYHAAAHYAASFALCSLAECVALWRTLGFAEDDALRALLP
                     MLSGTIETARDKGLPNALAGPVSRGDTGVVEKQLALLESLGGDHAALYGLLSRRAVEL
                     AERRAKPPVAIEAIAEAVEESLGRSLNQAAAGASVK"
     gene            5838..6128
                     /locus_tag="VHV99_13705"
     CDS             5838..6128
                     /locus_tag="VHV99_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636518.1"
                     /translation="MKKIVPFVVAAALGVGFATAAEAHVSVGIGIGIPVAPAYPVYAA
                     PPPVYYAPPPPVYYAPPPAVVVGGYYGGYGRPYYHHGYYRGYYGHGHGYYRH"
     gene            6278..7243
                     /locus_tag="VHV99_13710"
     CDS             6278..7243
                     /locus_tag="VHV99_13710"
                     /EC_number="4.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threo-3-hydroxy-L-aspartate ammonia-lyase"
                     /protein_id="HEX3636519.1"
                     /translation="MQALPVPTFDDVLDAAARLEGVAHRTPVLTSSTFNERTGASVFF
                     KCENFQRMGAFKFRGAYNAISHFDAEQRKAGVLTYSSGNHAQAIALSARLAGIRATII
                     MPHDAPAAKVAATKGYGGEVISYDRYTENREEIGRRLAEERGMTLIPPYDHPHVIAGQ
                     GTAVKELIEETGPLDMLFVCLGGGGLIGGSALSAAALSPACTVIGVEPEAGNDGQQSL
                     ARGEIVHIDAPRTIADGAASTHLGDYTFAIIRQRVAQIETVTDAQLIETLRFFAQRMK
                     IVVEPTGCLGAAAVLNGIVPVKGKRVGVVLSGGNVDLAKLAQYLA"
     gene            complement(7326..>7604)
                     /locus_tag="VHV99_13715"
     CDS             complement(7326..>7604)
                     /locus_tag="VHV99_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007589507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEX3636520.1"
                     /translation="ILWFAAVGGALIAKAGATSVTTAPVFLGGFFLGGLCWTIFICGL
                     GSHGRKRAGTGLLRACHVLSALLFAYFSYSVIVNGYRDLIVQTAQMAS"
BASE COUNT         1406 a         2292 c         2446 g         1460 t
ORIGIN      
        1 ttttccgttc atcccttcgc actacacgtt ctcgcagcag caggtgcagg acgcggtgca
       61 gcgcaaattc ccctatcagc gcacggtctc gcaagtgttc gatgtcgcgc tgacccaccc
      121 cgtggtcggt ttgctgccgg acgcgaatcg cgtctcggtg aagctcgacg cacgcctcgt
      181 cagtcccttc atgccgcagc cggtcgacgg cgtgttcacg ctgtcgagcg aactggctta
      241 tgacgccgcc agcaaatcgg ttgtgctcaa gtcgccgaac gtcgacaacg tcagcgtgag
      301 cgggcaggcg caggcctaca cgcagcagat caacgccgcg gcggcgctgc tcgccacgca
      361 gttgctgacc aactatccga tctacacctt caaacccgaa caacttcaat ttgccggagt
      421 gaattacgaa cccggtacaa tcaccatcct tacaaacggc atacgcgtgc aaatcgtcga
      481 aaaatgacgc tgtgcgcggc ctgcagggtc gcgcgcgcac tggccgactc tgttcgtcgt
      541 ttcgactatc tacagcgatg agggtcacgg atggactggc tattggcttg caaggcgctg
      601 attctgggcg tcgtcgaagg gttgacggaa tttttgccgg tctcgagcac cggccatctg
      661 atcgtcgcgg gcagtctgct taatttcacg gacgaacacg cgaaaacctt cgatgtcgtg
      721 attcagctcg gcgcgattct ggccgtctgc tgggagtttc gccggcgcat cggcagtgtg
      781 gtggtcggct tgcccagccg tcctgacgcg cgccgcttca cgctcaacgt gatcatcgcc
      841 acgattccgg caatcgtgct cggcctgttg ttcgaaaagg ccatcaaggc agcgctgttt
      901 tcgccggtgc cggtcgcgtt cgcgctggtg gcgggcggcg tggtgatcct atgggcggaa
      961 gcgcgccaac gcgcgcgcgg agacgcggcg gcgcgcattc agaacgtgga cgatctaggc
     1021 ccgctggatg ccttgaaagt cggcctcgct caatgttttg cgctgattcc tggcatgtcg
     1081 cgctcgggtt cgacgatcat cggcgggatg ctgttcggcc tcgagcggcg tgtcgccacg
     1141 gaattttctt tcttcctcgc gattccgatc atcttcggcg cgacggctta tgaactctat
     1201 aaagactggc atctgctgtc cgccgacgcg ctcggcagct tcgcgctcgg cttcgtggcg
     1261 gccttcatca gcgccttcgc gtgcgtgcgc tggctgctgc gctacatcgc cgcgcacgat
     1321 ttcacggtat tcgcgtggta ccggatcggc ttcggattgt tgattctgct ggtgggctat
     1381 agcggcgcat taagctgggc ggactgagtt tccgcgtggc ctttagccat gcaaaaaggt
     1441 ccgcggtggc ggaccttttt gcatgcgttg cttgctgccg gcgagttgcg aatgcgaagc
     1501 cgctttatgc tgcgccgcgc tttctgaaca ccaggtccca cacgccatgt ccgagtcgca
     1561 gcccgcggcg ctcgaacttc gtcacaggac gatactcggg acgcggcgcg tacccgtccg
     1621 ccgtattttc cagcgcgggg tcggcgccca gcacctcgag catctgctcg gcgtagtttt
     1681 gccagtcggt cgcgcaatgc agataggcgc ccggcttcag acgcgagacc agcaacgcga
     1741 cgaacttcgg ctgaatgagc cggcgcttgt gatggcgagc cttatgccac gggtccggaa
     1801 agaagatatg cacgccatcg aggctgtctg gcgcgatcat ctgttcgagt acctcgacgg
     1861 catcgtgttg aatgatgcgg atgttcgaca gcccttgctc ccccatcagc ttcagcagcg
     1921 cgcctacgcc tggctcgtgc acttccacgc cgaggaaatc gtcgtcggga cggtgcgagg
     1981 cgatttccgc ggtggtcgcg cccatcccga agccaatttc cagcacgcgc ggcgttgcac
     2041 ggccaaacac gctattccag tcgggttgct gcggtgtgaa gggcacgacg aaacgcggac
     2101 cgagttcgtc catcgcgcgg cgctggccgg tcgatacgcg gcccgcacgc gtgacaaagc
     2161 tgcggatgcg gcgtcgatgc aagcctgctt cttgcagggc gtcgttgtcg tcgttggcgt
     2221 tggccggttg gccttcggtg tcgggggaag tcggcgggag gccgtccggc aggccggcgt
     2281 cgtttggatc gtggatcatg gtcgatgctg ctacaagggg tcagtgcgcg gcggtttgca
     2341 tggcgttgcg aggcgccttc tgcgctcgcg cggcaagggc tgcggctcac aggcgcggtt
     2401 cgctcgaaac gccaggctgg ccgctgccat tacaaaaaag ccgccttcat gggaggcggc
     2461 tcgtgctgga ttctgctgaa ggctcagcag gaagatggag cggacgttgc aagtgcgcgt
     2521 aaggtagcac acccgaaaag ggtccgtcca gacggcggcc ggcctgaaag acggcgacgc
     2581 tattctcggg cggtatcaca cggctgcatc tgcccgtttg gtttgggaag caaatcgctg
     2641 gtgttgaaca cagcgagggc gcttcgcggg cttgtgcgcg tttgtgcgac ttctcggaag
     2701 acaggctgaa tgtaaagctg gcgggaattg ctgcagaatc agatcctttc gcgaagccgc
     2761 ctgggcaatc gccaggcggg ctgaaaagaa tctggagcgg gcgatgggaa tcgaacccac
     2821 ggctctagct tgggaagcta gggtattacc attatacgac gcccgcagag cgcacgattc
     2881 tactcgggaa ggggcggtta gcgcaaatcg cgctgccgcc cgcatgtcca cgcggtgcac
     2941 aaagcgccat tcagatcacg aagtaggtca actgaacggc cgcacgcgtg accaccatca
     3001 gcagcacctg cacgatcacg aacagcagaa tcggcgagag gtcaatgccg ccgagttgcg
     3061 gcacgatacg gcgcagtgga ttcaaaaacg gcgcggtcag ctggtacagg atcggcatgg
     3121 ccgacgagcg cggattgagc catgacagca gcgccatcag gatcgtcatc cagatgatca
     3181 gattcagcgc ccacttgatg agcgtcagca cagccacgat cagcaaggtc ggcgcgagcg
     3241 tgagcggatc ggcgcctgtg agcgccacca tcagcaccac ataaacgatt gccgccagca
     3301 gagccgcgac caggctggcc cagtcgatat tgcgcgtgct gtgcaggatg tgccgcaagg
     3361 gcagcacgat ccagttggtg acttgcagca cggcgttcga gacagggttg tacggcggca
     3421 ggcgcacgac ttgcagccat gcgcgcagca gcaacgcggc gccgaacagc gtaaagacgg
     3481 tattgagcag aaaacgggcg atatcgccaa acatctcgtg tccttatcct tcgaatgacg
     3541 gccgtcgatc ggccgcaagg tgccggcgct gaccgatcgg tgcgccggca gtttatttgg
     3601 cttgcccgcg ccgttcctgc aggagggcgc gtgacgcgtc ataaataggg acgcggtgga
     3661 tcaatttcca gatgttcgag tcaggccagc cggcgaaccg catgccgcca agccacacct
     3721 ggccgcggga catcacacag aaaccttgat ttcgctgatc agaatcgcgc cgttgccacc
     3781 gtcggtgtag ttcgggatgt cggcaagcaa ctgctccgag gccggcttga agactttgta
     3841 gaagtcgtcc gcgctatcga acaggaagtg acctgccgcg acatacggcg gcggcgttcc
     3901 cggcggcccg gcgttgatgc cgacgtcgac cgaccagcct ttcagcgacg gcccgaacag
     3961 cttcgccgcg agcggcatgt gcgtggcgca gtaatactcc acgtcgaagt ggccgttttc
     4021 ccgatatgga tagaggatgc tgaccttgat cattgtgcgt tctcgtctgt tggtcccgaa
     4081 gcgcggcgct ggcagattgc gcgcggcgcc ggacgtcaca ttatcacgcc ttacttgacg
     4141 cttgcacctg ctgcggcctg attcagtgac cggccgagcg attcttccac cgcctcggca
     4201 atcgcttcga tggcgacggg cggtttggcg cggcgttcgg ccagttcgac cgcgcgccgc
     4261 gacagcaagc cgtacaacgc ggcatggtcg ccgccgaggc tttccagcaa ggccaactgc
     4321 ttctccacga cgcccgtatc gccgcgcgac acggggccgg caagcgcatt gggcaggccc
     4381 ttgtcgcggg cggtttcgat ggtgccggac agcatcggca ggagcgcccg cagcgcgtcg
     4441 tcttcggcaa aacccagcgt gcgccacagg gcaacgcatt cagcgaggct gcacagcgcg
     4501 aaactcgcgg cgtaatgcgc ggccgcgtga tacagcatgc ggccgcccgc tgggatcgac
     4561 agcgggtggc agcgcagcgc gacggccagc gccgtcaggg cgtctttgag cgtgccgtca
     4621 gcctcgatcg ttaccgagca gccggcgatc cgttcgaggt cggcgagatc gccgccaaag
     4681 aggtacagtg ggtgaaaacc gccgatcgcg gcgccctggg cgcgcgccgg ggccagcaat
     4741 tcgaccggcg aggcgccgct gcaatgcacg agcgctttgc cgtgcgggcc gcttaccgca
     4801 tcggccgcga agcgcagtgt gtgtgctgtc gtaccgatgc tgtcgtcagg aacggctaaa
     4861 aagacgatat cggcggcgtc gacgacctgt tgttgattgt ccaatgccgc gcaatgctcg
     4921 atctgactgg cgagccggct ggccgaggcc ggcgtgcggc tcgccaccgc cgtgacggga
     4981 tagccggcgc gtgagaagct cagcgcgaga cagcgcgcga gccgccccgc accgataaag
     5041 ccgaggcgcg gcagagaggg gtgggacatg gttgcgtgct ccagacggga cacttgaacg
     5101 gggaaagcag aagtatcgcg catccgcccg gctgtggtgt gtcccggcgc aggcattcct
     5161 ctgatcagtg cgcgtgcgat atagcgcatc cggtcggtca tccgcccgcg cgcgccccgc
     5221 aggtccgaaa actgctcaca atttcttctt gagccggttt ggcagttgct gttctgggtt
     5281 ttttcgtctc gcagtcagat tcgggaggta acgatgaata ttttttctta ccgaaagcac
     5341 ttgcggttct tgacgcattc gcagcgccgt cgctggtggg aacagaaaaa gcgcttgacg
     5401 ccacgcgtgc gcatcagcgg cgcgtatgtc ccacccaacg tctccagaga attcgcctat
     5461 gtgaaggtag gctgaaaata ccgtctgctc acgagtaagg gatgcccgac gcgattgcgc
     5521 cgggcatttt tcatttgagc cgtcaggatt gaagcgaatt tgtaatcaaa gattactgac
     5581 aaacgggcgc ctgaagccgc aagcgccgca aggcagtaca ttcgcgtgcg ctccactgct
     5641 ggcaaacact tggttctcag tccgttacag cgcttgtccg cgcggggcag cgccgatgca
     5701 aattccgacc tattgccgtc gattgcaatt tgcaacaaat gcgaacatcc atgcgcagtc
     5761 tttttcgata cattgatttc gtagcatgca aggcatgcga agttggcgac cttgaccggc
     5821 cgcgccagga gaacaaagtg aaaaagatcg ttccgtttgt tgttgccgcc gcactcggcg
     5881 tggggttcgc tactgctgct gaagcgcacg tttcggtggg tattgggatt ggtattccgg
     5941 tcgcgccggc atatccggtt tatgccgcgc cgccgccggt ctattacgcg ccgccgccgc
     6001 cggtgtatta cgcaccgccg ccggcggtcg tggtgggcgg gtattacggt ggctatggcc
     6061 gtccttatta ccatcacggc tattaccgcg gttattacgg ccacggtcac ggctactatc
     6121 gccactgatc gaaaaagggg gcgtggagac aggttgacgg cgtaacgccg gcgtgatcgc
     6181 cgggttacgc cgtttgtgtt tttactgcga cgtaccaaag catcggcgaa gctgggagaa
     6241 tggctgtttt cgccaccgct cctggagaat ttcgccgatg caagcgcttc ccgttcccac
     6301 ttttgacgac gtactcgacg ccgctgcgcg tctcgaaggc gtcgcccacc gtacccccgt
     6361 tctaacttcc agcacgttca atgagcgcac gggcgcgtcg gtgtttttca agtgcgaaaa
     6421 ttttcaacgc atgggcgcct tcaagtttcg cggcgcctac aacgcgattt cgcatttcga
     6481 cgcggagcag cgcaaagcag gcgtgctgac gtactcgtcg ggtaaccacg cgcaagcgat
     6541 tgcgctatcg gcgcgccttg caggcattcg ggcgacgatc atcatgccgc acgacgcgcc
     6601 cgccgccaaa gtcgcggcga ccaagggtta cggcggagaa gtgatcagct acgaccgcta
     6661 caccgagaac cgagaagaga tcggccggcg gcttgccgaa gagcgcggca tgacgctgat
     6721 tccgccgtac gatcacccgc acgtgatcgc cgggcagggc acggccgtaa aggagttgat
     6781 cgaagaaacc ggcccgctcg acatgctgtt cgtttgcctt ggcggcggcg ggctgatcgg
     6841 cggcagtgca ttgtcggcgg cggcattgag cccggcgtgc accgtgatcg gcgtcgaacc
     6901 cgaagccggc aacgacggcc agcaatcgct cgcacgcggc gagatcgtgc atatcgacgc
     6961 accgcgcacg attgccgacg gtgcagcctc cacgcatctg ggcgactaca cgttcgccat
     7021 catccggcaa cgggtcgcgc agatcgaaac ggtcaccgac gcgcagttga tcgagaccct
     7081 gcgtttcttc gcgcagcgca tgaagatcgt ggtcgaaccg acgggttgcc ttggcgcggc
     7141 cgccgtgctg aatggcatcg tgccggtgaa gggtaagcgc gtaggtgtgg tgttgagcgg
     7201 cggcaacgtg gatctggcga agctcgcaca gtatctcgcc tgatagcggg cattgggctt
     7261 gacgccgccg cactgcgcac ggtggattga cggaagacgg cttgccaggg cgagccgttt
     7321 ttttattagc ttgccatctg tgcggtctgc acgatcagat cgcgatatcc gttcacgatc
     7381 acgctgtacg agaaatacgc gaacagcagc gctgacaaca catggcaggc gcgcagcaaa
     7441 ccggtgccgg cgcgtttgcg tccgtggctg ccgagtccgc agataaagat cgtccagcac
     7501 aagccaccca ggaagaagcc gccgaggaat accggtgcag tcgtcacgct ggtggcgccg
     7561 gctttcgcga tcaacgcgcc gccaactgcg gcgaaccaca gaat
//
LOCUS       DATASH010000497         6247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5814819,
            whole genome shotgun sequence.
ACCESSION   DATASH010000497 DATASH010000000
VERSION     DATASH010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6247
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5814819"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1524)
                     /locus_tag="VHV99_13720"
     CDS             complement(<1..1524)
                     /locus_tag="VHV99_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007733432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HEX3636521.1"
                     /translation="MIFDAENATAESSATVEPIVQLHPDDDVVIARHQLIAGMRIRSS
                     LVVRGLIPAGHKLAVRGIEAGAPVRRYGQIIGFATRVIEAGEHVHTHNLAMGDFDRDY
                     AFGADAKPASRAAAAARFMGIVRADGRVATRNYIGILTSVNCSATAARAIADYFRRDI
                     HPQALADYPNVDGVIALTHGQGCAIDSEGEALGLIRRTLGGYARHPNFAGVLIVGLGC
                     ETNQIDGLVQSQGLTPGAALKTMTIQATGGTARTVVAGIEQVKAMLVEANNVRREPVD
                     AKHPVIGLQCGGSDGYSGITANPALGAAVDRLVLHGGTAILSETPEVYGAEHLLTRRA
                     VSREVGEKLIERIHWWEAYCARMNADLNNNPSAGNKAGGLTTILEKSLGAVAKGGTTN
                     LVDVYRYAELVRAHGLVFMDTPGYDPVSATGQVAGGANLICFTTGRGSAYGCAPSPSL
                     KLGTNTALWQRQEEDIDLNCGSILDGERTVDELGEEIFQLMLATASGQASKSEIHGYG
                     "
     gene            complement(1695..3032)
                     /locus_tag="VHV99_13725"
     CDS             complement(1695..3032)
                     /locus_tag="VHV99_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3636522.1"
                     /translation="MQTQHVAQGPLESAVRKARLRLAPFLALMFALSMLDRSNVGFVK
                     QALQVDSNIGNAAFALGAGIFFIGYAVFEIPSNLMLHRVGAKLWLSRIMVTWGLASAA
                     MMFVHDETSFYVLRFILGVAEAGFSPGVILYSTYWFPARERGKALGIYYFGLPVALVL
                     GSPLSGYLMEVMGGRLGLHNWQWMFLIEGLAASTVGIVAFFYLVSKPRHAKWLTSAEK
                     DALENAIAVEDRDKVAHGPASALTALRNWNVLRFVAIYFAIQVSVYGVIFYLPTRISE
                     LTGTAIGSKVGLLTAIPWLCALIALRFITGYADAKGKHRQLAVVMLAMAAAGIALSTL
                     GSHLAPVLVAFCIATVGFVVVQPLFWTLPTAYLSGTAAASGIAMIGALGNLGGFIAPT
                     LKTAVETLFQSPRAGMFMLALAGVVGVLLLLSIGLHARRVTEAPIGSPKATLR"
     gene            complement(3100..4320)
                     /locus_tag="VHV99_13730"
     CDS             complement(3100..4320)
                     /locus_tag="VHV99_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX3636523.1"
                     /translation="MTSPLSLSVADTLPSDGLSGTLVGRAWIPATQSMPAGPAVVVLR
                     AEGVFDISDVAPTISSLVEREDPVATVRAASGRWIGTLDTIIANTAAATAGVARNPEH
                     GHLLAPCDLQVIKAAGVTFAASLLERVIEEQAKGDPQGAVRVREQIRALVGDSLTSIR
                     PDSAEARELKALLIEQGLWSQYLEVGIGPDAEVFTKAPVLAALGTGTEIGLHPNSVWN
                     NPEPEIVLSIDSRGRVLGATLGNDVNLRDFEGRSALLLGKAKDNNGSCAIGPFLRLFD
                     ETFSIDDVRRAPVDLRVEGSDGYVLRGTSSMDQITRDPLDLVSQTLSSTHQYPDGALL
                     FLGTLFAPIEDRDAAGAGFTHKLGDIVKISSRHLGTLVNRVNHSDRIEPWTFGVRALM
                     ANLAARGLLGTASL"
     gene            4479..5468
                     /locus_tag="VHV99_13735"
     CDS             4479..5468
                     /locus_tag="VHV99_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3636524.1"
                     /translation="MSRQFAVRTSSVLARLRFRHLQLLDALGRTHNLRIAAEQMLITQ
                     PAATKILSDIESMFGAQLFERLPREMRPTDLGVLSVRYATRAVADLGKFVSEFSTLSE
                     GGYGHLTVGAISASAAQVVITGIREIRQQRPQLVIKLVEQSSDQLAVWLEERKLDVMV
                     GRLTEPRHQVLFDFEVLSGEPVWVVVGQHHPLLQRRRLELADLGAWPWILYPQATAIR
                     QLFDETFAGAGIPAPVGMVETPSIFSTLELLQATDMISLQPRAAVEKYVSKGLLGHLP
                     VPINRIMTSYGIVTRKYELPSQPMQEFIAILRSVAGRVGDPAPPARGGSCEPT"
     gene            complement(5504..>6247)
                     /locus_tag="VHV99_13740"
     CDS             complement(5504..>6247)
                     /locus_tag="VHV99_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006758559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEX3636525.1"
                     /translation="KFGVPFSESDMVYFGLGFEQNRLDIDANTPQSYKDYVTQFGRVS
                     NNVPLTMGWSRDNRDSALVPSRGYYTQANAEYGTPLGDTPYYKADVQAQYYYSFARGF
                     VLGLNFQGGYGNGLDGKPYPIFKNYYAGGIGSVRGYEPSSLGPRDATTNDPIGGSRMV
                     VGNIELTVPMPGSGYDRTLRVFTFLDGGNVWGSEGNSIGANGLRYGYGVGLAWISPIG
                     PLKLSFGLPVVKHTGDQYQKFQFQIGTSF"
BASE COUNT         1227 a         1971 c         1858 g         1191 t
ORIGIN      
        1 gccgtacccg tgaatctcgc ttttcgatgc ctggcccgac gcggtcgcaa gcatgagctg
       61 gaagatctct tcaccgagtt cgtcgaccgt tcgttcgccg tcgagaatcg acccgcaatt
      121 caggtcgatg tcctcttcct ggcgttgcca caaagcggta ttggttccca gcttcaacga
      181 tggcgacggc gcacagccat aggcggagcc gcgaccggtt gtaaagcaga tcaggtttgc
      241 cccacccgcg acctgcccgg tcgccgaaac cggatcgtag cccggcgtat ccatgaaaac
      301 gagaccgtgt gcgcggacta attcggcgta tcggtacacg tcgacgagat tggtcgtgcc
      361 gcctttcgct acggcgccaa gcgacttttc gagaatcgtt gtcagcccac cggccttgtt
      421 cccggccgat ggattgttgt tcagatcggc attcatgcgc gcgcagtaag cttcccacca
      481 gtgaatccgt tcgatgagct tttcgccgac ttcacgcgac acggcccggc gcgtcaatag
      541 atgttcggcg ccgtagacct cgggggtttc ggagagaatc gccgttccgc catgcaacac
      601 cagacgatcg accgccgcgc cgagtgccgg attggcggta atgccggagt agccatctga
      661 accgccgcat tgcaagccga tcaccggatg ctttgcatcg acgggctcgc gccgtacgtt
      721 gttcgcttcc acgagcatcg ccttgacctg ttcgattccc gccaccaccg tgcgcgccgt
      781 gccaccggtt gcctgaatgg tcatcgtctt cagcgccgcg ccgggcgtaa gaccctggct
      841 ttgcaccagc ccgtcgatct gattggtttc gcagcccagc ccgacgatca gtacaccagc
      901 gaaattagga tgccgtgcat aacccccgag cgtgcgccgg atcagtccca gcgcttcgcc
      961 ttcggaatcg attgcgcagc cttgaccgtg cgtgagcgcg atcacgccat caacatttgg
     1021 atagtccgca agcgcttgcg gatgaatatc gcgacgaaag taatccgcga tggcgcgcgc
     1081 cgccgttgcg gagcaattga ccgatgtcag aatgccgatg tagttgcgcg tcgcgacacg
     1141 tccgtcagcg cgtacgatgc ccatgaaccg tgccgccgct gcggcccttg acgcgggctt
     1201 cgcatctgcg ccgaaagcgt agtcccgatc gaagtcgccc atcgccaggt tgtgcgtatg
     1261 gacgtgctcc ccggcttcga tgacacgtgt cgcaaagccg atgatctgcc catagcgccg
     1321 caccggcgcg cccgcttcga taccgcgcac ggcgagcttg tgacccgccg ggatcaaccc
     1381 acgtacgaca aggctactgc gtatgcgcat gcccgcgata agctgatgcc gcgcgatgac
     1441 gacatcgtca tccggatgca gttgcacgat tggctctacg gttgccgaag actcggccgt
     1501 tgcgttttcc gcgtcgaata tcatgacgta gcctcctcga tccgcgctgc gcgcactgcc
     1561 tcgcccgtta cggcatcaag gctgaagagc gcactattcg gtgcgcaagt gcagcgtcgt
     1621 ccgccgatgt aatgatgtcc gagaatgctc atgtgaattt ccggttaaat cgtgtgcagc
     1681 gtgcctgcat gtgtctagcg aagcgtcgct ttgggcgagc ctatcggcgc ttcggttacg
     1741 cggcgtgcat gcaagccgat gctcagaagc agaagcacgc cgacgacacc tgccaatgcc
     1801 agcatgaaca tgcccgcacg tgggctttgg aagagtgttt ccactgccgt ctttaaagtc
     1861 ggcgcgatga agccgccgag attgccgagc gcgccgatca ttgcaatacc gctcgctgcc
     1921 gcggtaccgc tcaggtaggc cgtcggcaag gtccagaaga gcggctgaac gacaacgaag
     1981 ccgaccgtcg cgatacagaa tgcgaccagc acgggtgcga gatgactgcc gagggtcgac
     2041 agtgctatcc ctgccgcggc catcgccagc atgacgacgg caagctgccg atgcttccct
     2101 ttcgcgtccg cgtagccggt gataaagcgc agtgcgatga gcgcgcacaa ccagggaatg
     2161 gcggtcagca agccgacctt cgagccgatg gcggtaccgg tcagctccga aatccgcgtc
     2221 ggcaggtaga agataacgcc gtatacgctg acctgaatgg cgaaataaat cgctacgaag
     2281 cgaagcacgt tccagttgcg cagcgcggtc agcgcactag ccggcccatg cgcgaccttg
     2341 tcccggtctt caactgcaat ggcgttctcc agggcgtcct tttcggccga cgtcagccac
     2401 ttcgcgtggc gcggtttgct gaccagatag aagaacgcga caatgccgac cgttgacgcc
     2461 gcgagacctt cgatcaggaa catccattgc cagttgtgca gcccgaggcg gccacccatg
     2521 acttccatca aataccctga cagcgggctg cccagcacga gcgccaccgg caaaccgaag
     2581 tagtagatgc ccagtgcctt gccgcgttca cgcgcgggga accagtacgt ggaatagagg
     2641 atgacgccgg gcgaaaagcc cgcttcggcc acgccaagaa tgaaacgcag cacatagaac
     2701 gaggtctcgt catgcacaaa catcatggcc gccgacgcaa gcccccaggt gaccatgatc
     2761 cggctcaacc agagctttgc acccacccga tgcagcatca gattgctcgg gatctcgaac
     2821 accgcatagc cgatgaaaaa gatcccggcc ccgagcgcaa acgccgcgtt gccgatgttc
     2881 gaatcgacct gaagagcctg tttgacaaac ccgacattcg aacggtcaag catcgatagc
     2941 gcgaacatca gcgcgaggaa cggcgcgagc cgcaggcgcg ccttgcgcac cgcgctttcc
     3001 aacgggcctt gcgcaacgtg ttgagtctgc atctcgtctg tctccttgaa gccgggtatc
     3061 ccggtctttt atggcgggtt agagcgggtt catacgcgat cagagcgagg ctgtgccgag
     3121 cagtcctcgc gcagcaaggt tcgccatcag cgcgcgcacg ccgaaggtcc acggctcgat
     3181 gcggtcgctg tggttgaccc ggttgaccag cgtgccgaga tgccggctcg aaatcttcac
     3241 gatatcgccc agcttgtgtg tgaaaccagc accagcggcg tcgcggtctt cgattggcgc
     3301 gaaaagcgtc ccgaggaaca gcagcgcgcc atcggggtac tggtgggtcg aactgagcgt
     3361 ctgcgaaacg agatcgagcg ggtcgcgcgt aatctggtcc atcgaactcg tgcctcgcaa
     3421 gacataaccg tcgctgccct ccacacgcaa atcgaccggc gcgcggcgca catcatcgat
     3481 cgagaaagtc tcgtcgaaaa ggcgcaggaa agggccgatc gcacacgagc cattgttgtc
     3541 cttcgccttg ccaagcagga gcgcactgcg tccttcaaag tcacgcagat tgacgtcgtt
     3601 gcccagggtc gcgccgagca cgcggccgcg actgtcgatc gacagcacga tctccggctc
     3661 cgggttgttc cagaccgaat tcggatgcag cccgatctcc gtgcccgtgc ctaacgccgc
     3721 caacaccggc gctttggtga agacttccgc gtcagggccg attccgactt ccaggtactg
     3781 ggaccatagc ccctgctcga tcagcagcgc cttcaattcg cgcgcctcag ccgagtccgg
     3841 ccggatgctg gtgaggctat cgccgacgag agccctgatc tgctcacgca cgcgcaccgc
     3901 gccttgcgga tcgccctttg cctgctcctc gatcactcgt tcaagcaggc tcgccgcaaa
     3961 cgtcaccccg gctgccttga tgacctgcag atcgcaaggc gcaagcagat ggccatgctc
     4021 cgggttgcgc gccacgccag ccgtcgcggc ggccgtattg gcgatgatcg tgtccagcgt
     4081 gccaatccag cggccactcg cggcgcgtac tgtcgcaacc ggatcttccc gctccaccag
     4141 gctgctgatg gtcggcgcca cgtcggagat gtcgaacacg ccttcggcgc gcaagaccac
     4201 gacggccgga cccgcaggca ttgattgcgt ggcgggaatc caggcacggc cgacaagtgt
     4261 gccggacaac ccgtccgacg gaagcgtgtc cgctacgctg agtgaaagcg gggatgtcat
     4321 gtctgtctcc acagatgttt gtctggcgcg cgcggctggc tgcccgttcg cgcgttgatt
     4381 cgcccagctg ccactttagg cagccacgcg ataaacatcc aatctcttat tcagtacaat
     4441 cgattccatc cggttatgag ttcttgcgtg tttaccccat gtccagacaa tttgcggtgc
     4501 gtacgtcgag tgttctggca aggttgcgct tcagacatct tcaactgctc gatgcccttg
     4561 gacgcacgca caacttgcgc atcgcagccg agcagatgct cattacccag cccgcggcga
     4621 ctaaaatcct gagtgacatc gaatcgatgt tcggcgccca gttgttcgaa cggttaccac
     4681 gcgagatgcg tccaaccgac ctcggcgtat tgtccgtgcg ctacgcaacc agggcggtcg
     4741 cggatctggg caagttcgtg agcgagttca gcaccttgag tgagggtggc tacggtcatc
     4801 tcaccgtggg cgccatctcg gcttcggccg ctcaggtggt gattacgggc atcagggaga
     4861 tccgccagca gcggccgcaa ctggtcatca aactggtcga gcaaagcagc gatcaactgg
     4921 cggtctggct cgaagaaagg aagctggacg tgatggtcgg ccgtctgacg gagccgcgcc
     4981 atcaagtcct gttcgacttc gaggtgctgt ccggggaacc ggtctgggtg gtcgtcgggc
     5041 agcatcatcc gctgctccag cggcggcggc tggagctggc cgatcttggc gcgtggccat
     5101 ggattctcta tccacaagcc acggctatca ggcagctatt cgatgaaacg ttcgccggcg
     5161 cgggcattcc cgcgccagtg ggcatggtgg aaacgccttc aatcttttcg acgctggaac
     5221 tgttgcaggc gaccgacatg atctcgctgc aaccgcgtgc cgccgtcgaa aaatatgtga
     5281 gcaagggtct gctcgggcac ctgcccgttc ccatcaaccg gatcatgacg agctacggta
     5341 tcgtcacgcg caagtacgag ttgccgtccc agcccatgca ggagttcatc gccattttgc
     5401 gttcggtcgc cggacgtgtc ggcgatccgg cgcctccagc gcggggcggc tcgtgtgagc
     5461 cgacctgacg atacgaatcg gccaaagcgt attgacgata gcctcagaac gatgtgccta
     5521 tctggaactg gaatttctgg tactggtcgc ccgtgtgctt gacgaccggc aagccgaagc
     5581 tcagcttgag cggaccgatc ggcgagatcc aggcgagacc caccccatag ccgtagcgca
     5641 atccgttcgc gccgatgcta ttgccttcgc tgccccaaac attgccgccg tcgaggaagg
     5701 taaagacgcg caacgtgcgg tcataaccgg atcccggcat tggaacggtc agctcgatat
     5761 tgccgacgac catcctcgag ccgccgatgg ggtcgttggt ggtcgcgtcg cgcggcccga
     5821 gcgaacttgg ctcatagcca cgcactgaac cgataccgcc cgcgtagtag ttcttgaaga
     5881 tcggataggg tttgccgtcc aatccattac cgtagccacc ctgaaaattc aggccaagca
     5941 cgaagccgcg cgcgaatgaa taatagtatt gcgcctggac gtctgccttg tagtacggcg
     6001 tatcaccgag cggcgtgccg tattccgcat tggcttgcgt atagtagccc cggctcggga
     6061 caagcgcgct atcacggttg tcacgcgacc atcccatcgt caacggcaca ttgttggaga
     6121 cacgcccgaa ttgcgtgacg taatccttat agctctgggg tgtattggca tcgatatcca
     6181 ggcggttctg ctcgaagccc aggccgaagt acaccatgtc ggactcggag aaaggcacgc
     6241 cgaattt
//
LOCUS       DATASH010000498         2053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1068684,
            whole genome shotgun sequence.
ACCESSION   DATASH010000498 DATASH010000000
VERSION     DATASH010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1068684"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            357..1319
                     /locus_tag="VHV99_13745"
     CDS             357..1319
                     /locus_tag="VHV99_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3636526.1"
                     /translation="MELKWLEDFVSLAETRSFSRSAESRHVTQPAFSRRIQALEAWLG
                     TELIDRSVYPTRLTAAGQVFYEQALAMLSQFHEARALLRGHTATPQATIEFAVPHTLS
                     LTYFPRWLQRIEAQMGPIHTRLRALNVHDAALALVEGGCDLMMGYHHPSHPVALDPAR
                     YDMLTLGNEPISPFSAPARAGRPRHTLPGSADAPAPYLTYTPNAYLGRMTEVILTNAP
                     ERLYLDRLYETDMAEGLKAMALAGHGIAFLPHSAVEDAVADGKLIRLDRATRGTPEGQ
                     LTLTMEIRLYRDKLAAKSDDARQILVRQLWDVVSEELAQGAGAA"
     gene            1458..>2053
                     /locus_tag="VHV99_13750"
     CDS             1458..>2053
                     /locus_tag="VHV99_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEX3636527.1"
                     /translation="MSSQQQVAQSASSLQSVPSYLNSEDLGPWGNYLRQVDRVAPYLG
                     SLSRWLETLKRPKRILIVDVPIELDNGTVAHFEGYRVQHNVSRGPGKGGVRYHQDVTL
                     SEVMALSAWMSVKNAAVNVPYGGAKGGIRVDPRTLSRGELERVTRRYTSEIGIIIGPN
                     TDIPAPDVNTNEQIMAWMMDTYSMNQGQTATGVVTGKP"
BASE COUNT          382 a          635 c          676 g          360 t
ORIGIN      
        1 caaccgtcac gcgattgcgc atgaaagcgg cttccgagag ccagtcgcgc agggcttcgg
       61 ttttcgaggc gtagcggccg tcgagcgggg agagcgcgtt cagcgcgaac agggtgtcgg
      121 ggcgggtgtc ggacatgatg ggggcgggtt ggggcgttga acgcccggtg agcgctcaat
      181 gaccagttct ggaacggagg gaagcgcgaa ttttaccatc gcgcggcttt tcgcccgaag
      241 ttggccgcga accgtctatc tgcatcgcac atcagcccgc gcgtttggaa tcgcggcacg
      301 ccagccgcgc cgctagaatg ctgactcctt ccctcccgcc ggcagcaggc tttcgcatgg
      361 aactgaaatg gctcgaagac ttcgtttcgc tcgcggaaac gcgtagtttc agccgctcgg
      421 ccgaatcgcg tcacgtcacg caaccggctt tttcgcggcg gattcaggca ctggaagcgt
      481 ggctcggcac ggaattgatc gaccgttcgg tttacccgac gcggctgacg gcggcaggcc
      541 aggtgttcta cgagcaggcg ctcgcgatgc tgtcgcagtt tcacgaggcg cgcgcactac
      601 tgcgtgggca cacggcgacg ccgcaggcga ccatcgagtt cgcggtgccg cacacgctgt
      661 cgctcaccta ttttccgcgc tggctgcaac gcatcgaggc tcagatgggc ccgatccata
      721 cccggctgcg ggcgttgaac gtgcacgacg cggcgttggc gctggtggaa ggcggctgcg
      781 atctgatgat gggctatcac catccgagtc atccggtcgc gctcgatccg gcgcgctacg
      841 acatgctcac gctcggcaac gagccgatca gtccgttctc ggcgccggcg cgggccggca
      901 ggccgcgcca cacgctgccc ggcagtgccg acgcgccggc gccgtatctg acgtacacgc
      961 cgaacgccta cctcggccgc atgaccgaag taatcctgac caacgcgccg gagcgcctgt
     1021 acctcgaccg gctgtacgaa accgacatgg ccgaggggct caaggcgatg gcgttggctg
     1081 ggcatggcat cgcgttcctg ccgcatagcg cggtggaaga cgcggttgcc gacggcaagc
     1141 tgatccgcct cgaccgcgcc acgcgcggca cgcccgaagg acagctcacc ttgacgatgg
     1201 agatccgcct gtatcgcgac aagctcgcgg cgaagagcga cgatgcgcgg cagatactcg
     1261 tgcggcagtt gtgggacgtc gtgtcggagg agttggcgca aggcgcgggc gctgcctgaa
     1321 aacctgcttg cctacgacgt gaatttgcag ctttttgacg gttatgcaaa aaaaacataa
     1381 tcggataagg aaacggcatt ggatttcaaa accgcgtttt tcgacaatgg cgctattcga
     1441 tcaacgccgg agcgttcatg tcttcacagc agcaagtcgc acaatccgca tcatcgttgc
     1501 agtccgttcc ctcctacctc aatagcgaag acctcggccc gtggggcaac tacctgcgtc
     1561 aggtcgaccg cgtcgcgccg tacctcggct cgctgtcccg ctggctcgaa accctgaagc
     1621 gcccgaagcg cattctgatc gtcgacgtgc ctatcgaact cgataacggc acggttgcgc
     1681 acttcgaagg ctatcgcgtg cagcacaacg tgtcgcgtgg tccgggcaag ggcggcgtgc
     1741 gttatcacca ggacgtgacg ctgtcggaag tgatggcgct gtcggcgtgg atgtcggtga
     1801 agaacgcggc ggtgaacgtg ccgtacggcg gcgcgaaggg tggtattcgt gtcgatccgc
     1861 gcaccttgtc gcggggcgag ctggagcgcg tgacgcgccg ctacacgagc gaaatcggca
     1921 tcatcatcgg accgaatacc gacatccccg cgccggacgt gaacacgaac gagcagatca
     1981 tggcgtggat gatggacacg tactcgatga accagggcca aacggccacc ggcgtcgtga
     2041 ccggcaagcc gat
//
LOCUS       DATASH010000499         5841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5618334,
            whole genome shotgun sequence.
ACCESSION   DATASH010000499 DATASH010000000
VERSION     DATASH010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5841
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5618334"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(431..604)
                     /locus_tag="VHV99_13755"
     CDS             complement(431..604)
                     /locus_tag="VHV99_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636528.1"
                     /translation="MDAESIAGLIGLAIGLLVLLALSIFESRTYRREHDGEGMLHHWL
                     THQHMPHWMRRRH"
     gene            760..924
                     /locus_tag="VHV99_13760"
     CDS             760..924
                     /locus_tag="VHV99_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636529.1"
                     /translation="MNRVLLIALMLAAGLGGCANDPSGHHGGTPPKDPADYHGVPTDD
                     RPPAMVPVAP"
     gene            complement(988..2340)
                     /locus_tag="VHV99_13765"
     CDS             complement(988..2340)
                     /locus_tag="VHV99_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX3636530.1"
                     /translation="MGSGAAATAPAVPVRWHRRVLTLAFPIVLANLTQPILGAVDTAV
                     AGHLDGASYLGGVALGGLFFNFVFWGFGFLRMGTTGLVAQAHGAGDHAELRNNVVRAL
                     LLAAAIGAAVLLLQGPLIGYALRAIGGSDAVQRHAQVYCRARIWAAPLALGNYVVLGW
                     LLGTQQVRLALLSQVFINSVNIAAVLSYVYVFDWGVAGIGAATATADALGFILGAALL
                     WHGRPRGLPALNRTALFDASALKRLVVLNRDIFVRTLCLLSSFGWFAHLGARQGDATL
                     AANALLLNFQTFMAYGLDGFAHAAEALVGAAIGARDRHAFAQAIKVTALWSALGALGF
                     SLVYWGAGSWIIERLTDQAAVRATAETYLPWAALSPVISVWGFLLDGVFIGATRTREL
                     MMSMVVSFAVFVAASWALLTLYGNHGLWAAMLIFMAARGVTLARFLPGVVRGMARAGA
                     "
     gene            complement(2373..2681)
                     /locus_tag="VHV99_13770"
     CDS             complement(2373..2681)
                     /locus_tag="VHV99_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2288 domain-containing protein"
                     /protein_id="HEX3636531.1"
                     /translation="MTSNDATHSPLYAKLLAETAKIGWSELERFFARGMLLRVARDID
                     LVSVAEAIASDDTTQVTQWLSAGLVERVQAETAADFAARDPDLWAVVVSPWVCVQERN
                     "
     gene            2790..3518
                     /locus_tag="VHV99_13775"
     CDS             2790..3518
                     /locus_tag="VHV99_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEX3636532.1"
                     /translation="MSLDSTAPDSGAQAHVVTGAALTRTDVERLIAANLAQSKEPLHF
                     TDCDFEGADLSRLDLRGAEFHRCTIVETSFFGATLSHTRWVRCRGREADFASADLVDA
                     AFQSSDLNNTSWRRAKLASASFHSCKLTGANFEHVAALGLSFVETLLVGAHLRGLSFR
                     KATLQQLDFSDADLAGVDFREAIFDGGSLKDAHLKGARFDGADLREADLSGVRLVDAA
                     LFKGATISHQQAAVLVTELGLRVM"
     gene            complement(4376..4702)
                     /locus_tag="VHV99_13780"
     CDS             complement(4376..4702)
                     /locus_tag="VHV99_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636533.1"
                     /translation="MKSVFASMATATAIGLGLLTLGGLGGCSSTTTMTYLPSGDTGFA
                     INCSGSDASSSWAECYKRAGEVCGNYGYDVVSKDVDNGATSGGTVGGIFGANVKNRSM
                     VVRCKQ"
     gene            complement(4830..5555)
                     /locus_tag="VHV99_13785"
     CDS             complement(4830..5555)
                     /locus_tag="VHV99_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX3636534.1"
                     /translation="MLNRRRMMKRLACASLLAVLAACGPAPVAPGFYRVERGDTLSKI
                     ARSNRQSVQSIVRWNSLTNPDSIEVGQVLRVAPPGGGASGGTSGGAIRSSGAGSASAS
                     AAPRTAPSDNAPASAPASTISLVWPANGTVIRRFDGGNSKGIDISAAAGTPVIAAAPG
                     TVVYAGNGLRGYGNLLIIKHNAEYLTAYAHNRALLVKEGQSVTRGEKIAEMGDTDTDR
                     VMLHFELRYQGRSIDPSRALPPR"
BASE COUNT         1085 a         1804 c         1872 g         1080 t
ORIGIN      
        1 tcgggccgcg tggtctgttg gttcggcgcc tgcgcgccgg gacgagggac gcgcatcgct
       61 acgcctcctt gcaggatgct cgtcagcaag gcatgtgcct gcgaggattc cagcgggtga
      121 ggtagagtgg aagtactgca gggtggaagt aatgcaggga gggagcgtgc aaggcaggga
      181 gtatgcaagg cagggagtat gcaaggggag gaagcacgca agaaaggaag cctgcaaggc
      241 aggaatcgcg cagggaggat acacgcaaga aacgaagcgc agatgacgaa gtacggcagg
      301 tgggcgagtg gcgtgcggca ggccgtgtac cacaggcgcg tgagcagcgg acacgaatgc
      361 gttgcgcgtc agcggacgct cgcgcttgcc tgtcgtacgc aagcgcgcga gcagcagccg
      421 ggtgattccc tcaatgccgg cgacgcatcc agtgcggcat atgctggtgt gtgagccagt
      481 gatgcagcat gccttcgccg tcgtgttcgc gccgataagt tctcgattcg aaaatcgaca
      541 gcgcgagcag cacgagcaga cctattgcga gtccgatcag gcctgcaatc gattcagcgt
      601 ccatggcagt ctcctatgac acgtagctgt gtctttatag tagatcacgc gtaacaaaat
      661 ggcagcaggg agagtgtgag gcacgagttc gtcgaaggcg aattgcgggt agactttcgg
      721 cgcttcgtcg ccgttattgt cattctgtct cgagagtcta tgaaccgcgt tttgttgatt
      781 gcattgatgc tggccgccgg actcggcggc tgtgccaacg acccttccgg gcatcatggc
      841 ggcacgccgc ccaaagatcc cgccgactac cacggcgtcc ccaccgacga ccggccgccc
      901 gccatggtgc ctgtcgcgcc ttgagggcag acacgggcaa atgaaagcga agttccggcg
      961 cgggcacgcc gggcgcctaa gcaaagccta agcgcccgcg cgcgccatac cgcgcacgac
     1021 gcccggcaaa aagcgcgcaa gcgtgacgcc gcgggcggcc atgaagatca gcatcgcggc
     1081 ccacaagccg tgattgccat acagcgtcag caaggcccac gaggccgcca cgaacaccgc
     1141 aaacgacacc accatcgaca tcatcagctc acgcgtgcga gtcgcgccga tgaacacgcc
     1201 gtcgagcaga aagccccaca cggagatcac cggcgaaagc gcggcccagg gcagataggt
     1261 ctcggccgtg gcgcgcaccg cggcctgatc ggtcagacgt tcgatgatcc atgagcccgc
     1321 gccccagtac accagcgaga aacccagcgc gccgagcgcc gaccacagcg ccgtcacctt
     1381 gatggcctgc gcgaaagcgt gccggtcgcg cgcgccgatc gccgcgccga ccagcgcttc
     1441 ggcagcgtgc gcgaagccgt cgagtccgta ggccatgaag gtctgaaaat tgagcagcag
     1501 cgcgttggcg gcgagcgtgg cgtcaccttg tctcgcccca agatgcgcga accagccgaa
     1561 cgacgacagc agacagagcg tgcgcacgaa gatatcgcga ttcagcacca cgagccgctt
     1621 gagcgcggac gcgtcgaaga gggcggtgcg gttcagcgca ggcaagccgc gtggccggcc
     1681 gtgccacaac agcgcggcgc cgagtatgaa gccgagcgcg tcggccgtgg ccgtggccgc
     1741 gccgataccc gccacgcccc aatcgaaaac gtacacatac gacagcaccg ccgcgatgtt
     1801 cacgctgttg atgaacactt gcgaaagcag ggccagccgc acctgctgcg tgccgagcag
     1861 ccagccgagc accacgtaat tgccgagcgc gagcggcgcg gcccagatac gcgcgcggca
     1921 gtagacctgc gcgtggcgct gcaccgcgtc gctgccgccg atcgcgcgca acgcgtaacc
     1981 gatcagcggc ccttgcagca gaagcaccgc tgcgccgatc gccgccgcga gcaagagcgc
     2041 gcgtacgacg ttgttgcgca actcggcatg gtcgcccgcg ccgtgcgctt gcgcgacgag
     2101 gcccgtggtg cccatgcgca ggaagccgaa gccccagaac acgaagttga agaacagccc
     2161 gccgagcgca acgccgccga gataggaggc gccgtccaga tggccggcga cggcggtgtc
     2221 gaccgcgccg agaatcggct gtgtgagatt ggcgaggacg attggaaacg ccagcgtcaa
     2281 cacccggcgg tgccagcgca ccgggacggc gggcgccgtc gcggccgcgc cgcttcccac
     2341 aaagcgctcc ttcggggcgg gcgcacggtc gctcaattgc gttcctgcac gcacacccat
     2401 ggcgagacga ccactgccca caggtccgga tcgcgcgcgg cgaaatcggc ggcggtttcc
     2461 gcctgcacac gttcgacgag gccagcagac agccattgcg tgacctgcgt ggtgtcgtcg
     2521 ctggcgatgg cttcggcgac gctcaccagg tcgatgtcgc gcgcgacgcg cagcaacatg
     2581 ccgcgcgcga aaaagcgttc gagttcgctc cagccgattt tggcggtttc ggcgagcagc
     2641 ttggcgtaca gcgggctatg agtggcgtcg ttggaagtca tcgtaggcgg gttctgacgg
     2701 ggccgttact ataaaccatc ggcgtggagc gccagggcgg agcgcgttcc ggtacgctta
     2761 ggatctttcg aaatggattc tgaaccttca tgtcactgga ttcaacggcg ccggattcgg
     2821 gcgcgcaagc tcacgtggtg accggtgcgg cactcacgcg taccgacgtc gagcgtctca
     2881 tcgccgcaaa cctcgcccag agcaaagagc cgctccattt caccgactgc gatttcgaag
     2941 gcgccgatct gtcgcgactc gatttgcgcg gcgctgagtt tcatcgttgc acgatcgtcg
     3001 agacctcgtt tttcggcgca acgctgtcgc acacccgctg ggtccgttgc cgtggccgcg
     3061 aggcggattt cgcgtcggcc gatctggtcg acgccgcgtt ccagtcgagc gatctgaaca
     3121 acaccagctg gcggcgcgcc aagcttgcct cggcgtcgtt tcacagctgc aagctgaccg
     3181 gcgcgaactt cgagcatgtc gctgcgctgg gcctgagttt cgttgagacg ttgctggtgg
     3241 gcgcgcattt gcgcggcttg tcgtttcgca aggccacgct gcagcaactc gatttctccg
     3301 acgccgatct ggccggggtc gatttccgcg aggcgatctt cgacggcggc agtctgaagg
     3361 atgcgcatct gaagggcgcg cgcttcgatg gagcggactt gcgggaagca gacctgagcg
     3421 gcgtgcggct tgtcgatgcg gcgctgttca aaggcgcgac gatctcgcat cagcaggcgg
     3481 cggtgctcgt gactgaactc ggtctgcgag tcatgtgatc gatgtcttta gccggccgat
     3541 ttttagccat tcggccagct tgaacggctc gatatatgtg cttatcatct gatggttgaa
     3601 tcagttaata acggtcgatt ttagacttag ccatttgtcg gttgatctga agatcgttaa
     3661 tatatccgcc attcgcgaat ttgttgtgaa gaaatgcgat ttctattcgc gtcgctcaat
     3721 atccgctaat acttcagtat ggtgcgttgg cgcactaaag attcgcaatc tataaagcgt
     3781 ccgaatcata ctgggcgtgg ctagcgcaaa acggccaagg aactttcgtc agatttttcc
     3841 tatcgcgtat aggcagatcc ttgattaata aggtcctgcg agcagatcgc ggcattctga
     3901 tccgcccttt aaatggcgtt tcatacggag tggacaatgg tatttattcg cttatttccg
     3961 acatttttcg ttcacgtaat gcggactccg tacgcagtgt cacactgcac gcgaaattaa
     4021 tagattgcgc ttgtaaaggc gggttgttag atttcgttct ggcgcatggt gaaccggtcc
     4081 tttgccgata tgcgccgaac ccccgttagc ccggcctcgt gccgggcttt ttttcgtccc
     4141 ggtcattgct gattcactgt acggtcactg cggattcaca cgagacgcgg attatggaaa
     4201 gttacatttt cttaccgccg tcatgacttg gttgatcatt ttcctcggca tctggccgat
     4261 cgcgcttagt gtgctgttcg ccttcgccgc ccgcatcgat ccgcgctccg gccgctggcg
     4321 gcgccgctag cgcgagccgc gctcggcctt gggtgcgcgc tgttccatgt ggccttcact
     4381 gcttgcagcg aactaccatc gaccggttct tgacgttcgc gccaaagatt ccgccgaccg
     4441 tgccgcccga ggtggcgccg ttgtccacgt ccttcgaaac gacgtcgtag ccatagttgc
     4501 cgcagacttc gcccgcgcgc ttgtagcact cggcccaact ggagctcgcg tcgctgccgc
     4561 tgcagttgat cgcgaagccg gtatcgccgc tcggcaggta ggtcatggtg gtggtgctgg
     4621 agcagccgcc gaggccgccg agcgtcagca ggccgaggcc gatcgccgtg gccgttgcca
     4681 tggacgcaaa tacgctcttc attgcagatt ccttacagaa aacgggggaa gacgtggcat
     4741 caagccacgt catgcatagg attgagcgtt ttatagcacg cgcggttccc tcggctgcgg
     4801 ttgcagggtg cgtgcctgac gctggatggc tagcgaggcg gcagtgcccg cgaggggtcg
     4861 atcgagcggc cttgatagcg cagttcgaag tgcagcatga cgcgatcggt gtcggtgtcg
     4921 cccatttcgg cgatcttctc gccgcgcgtg accgattgac cttccttcac cagcaacgcg
     4981 cggttgtgcg cgtaggccgt caggtactcg gcattgtgct tgatgatcag caggttgccg
     5041 taaccgcgca agccgttgcc tgcgtagacc accgtgccgg gcgccgcagc tatcaccggc
     5101 gtgcccgctg ccgccgaaat atcgatgccc ttcgagttgc cgccgtcgaa gcgccggatc
     5161 accgtgccgt ttgccggcca taccagtgaa atcgtcgaag ccggcgcgga cgccggcgca
     5221 ttgtcgctcg gagccgtgcg aggcgcagcc gacgcgctcg cgctgcccgc accgctgctg
     5281 cggattgcgc cacctgaggt gccgcctgaa gcgccgcccc ctggcggcgc gacccgcaac
     5341 acttgtccca cctcgatgct gtccgggttg gtcagactgt tccagcgcac gatgctctgc
     5401 accgattgcc ggttgctgcg cgcgatcttc gatagcgtat cgccccgttc gacgcgataa
     5461 aagcctggcg ccaccggagc gggcccgcag gcggcgagca ccgccagcaa cgacgcgcag
     5521 gcgagccgtt tcatcattct tcttctgttg agcattggac ctcgcaaagc tctgccgcgc
     5581 gccgatcccg atcacgcggc aggtagatgc aaagcgtcag attctaacgg ttttgcgctg
     5641 cagcatccga aaaacgggcg gggaggcggg cgcacacagc aggaacgtgg caatacgcca
     5701 cgaggagccg cgcaatcgat cacgtcgcga tgctcgcaaa tcctgcggtg tgacccttgc
     5761 tattcgggca gcttgctgac gctgatgcgc agtgtggccg tctcggtttg tcccggttca
     5821 agccagcgca atcccatgcc g
//
LOCUS       DATASH010000500         4072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_7002079,
            whole genome shotgun sequence.
ACCESSION   DATASH010000500 DATASH010000000
VERSION     DATASH010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7002079"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..142)
                     /locus_tag="VHV99_13790"
                     /pseudo
     CDS             complement(1..142)
                     /locus_tag="VHV99_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004525489.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            complement(319..1452)
                     /locus_tag="VHV99_13795"
                     /pseudo
     CDS             complement(319..1452)
                     /locus_tag="VHV99_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007743037.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
     gene            complement(1525..2973)
                     /locus_tag="VHV99_13800"
     CDS             complement(1525..2973)
                     /locus_tag="VHV99_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX3636535.1"
                     /translation="MSYRTEEVAYVRPAAQSSTAASVQTTQQQRSTAEYRALDAAHHI
                     HPFSDMGSLNRAGSRVIVKAQGVYLWDSEGNKVIDGMAGLWCVNVGYGRKELADAAYK
                     QMQELPYYNTFFKTTHPPVIELSALLAELAPEPFNHFFYCNSGSEGNDTVLRIVHQYW
                     ATQNRHAKKFVISRKNGYHGSTIAGGTLGGMGYMHEQMPSKVENIVHIDQPYFFGEAE
                     GNQTPEEFALARAQQLEAKILEIGADNVAAFIGEPFQGAGGVIFPASTYWPEIQRICR
                     KYDILLVADEVIGGFGRTGEWFAHQHFGFEPDLITLAKGLTSGYVPMGAVGLHDRVAT
                     AIIENGDFNHGLTYSGHPVAAAVALANLKLLRDEKIVERVKTDTGPYFQKKLRDTFAN
                     HPIIGEIAGAGLVAGLQLAEDPKTRKRFANGGDVGTICRDFCFNGNLIMRATGDRMLL
                     SPPLVITKLEIDEIVSKARKAIDATAQQLGIS"
     gene            complement(3035..>4072)
                     /locus_tag="VHV99_13805"
     CDS             complement(3035..>4072)
                     /locus_tag="VHV99_13805"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011658120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEX3636536.1"
                     /translation="HFDGTPVAISPRRVLRQVLELYKAKGWKPVIAPELEFYLVDMNK
                     DPDLPLQPPIGRTGRAETGRQAYSIEAVNEFDPLFEDIYEYCEVQELEVDTLIHEVGA
                     AQMEINFMHGDPLKLADSVFLFKRTVREAALRHKMYATFMAKPMEGEPGSAMHMHQSL
                     VDEESGHNVFTSADGKPTSLFTSYIAGLQKYTPALMPIFAPYINSYRRLSRFMAAPIN
                     VAWGYDNRTVGFRIPHSAPAARRIENRIPGVDCNPYLAIAATLAAGYLGMTQQLDATE
                     PLLSNGYALPYQLPRNLEEGMTLMASCEPIAEVLGDKFVKAYLALKETEYEAFFRVIS
                     SWERRHLLLHV"
BASE COUNT          743 a         1174 c         1265 g          890 t
ORIGIN      
        1 gcaggtcctt cattcgatac caggccatcg cgaggacgag ggcaggggtg cgcagtgtag
       61 cgccgcccgg gaagtcgcgg tgacgaatct tgccgaacag atcgaaacgc gacgcctggc
      121 cgtcgatcgc ttcggcgatc aaagtgtctc cctgcgaggc gcgggaagcg gctcgcaaga
      181 tgcgctgcga ttggaaaaca ggggactgcg caggtgggac ctaaaaacgc gcaggctgac
      241 taccgttact gcttttcgta cgctcagggt tcaagcgatc cctgagcgcg gaaaacgaaa
      301 cgctttaagc tggcttaatc aatggccggt tttaagctgc gcccaaagac ggttttccag
      361 acgcagaata tcggtgggca tcggcttcat gagggtcatc ttgctcaata cgtcgtcgcc
      421 cggataaacg gttttgtcct gggcgactac cggcgtgacg aactggcgcg cggccttgtt
      481 cgcagtcggg tagaacactt cattggtgat cgcggcgttg accttcgggt ccgagatgta
      541 attgatccac ttcagcgcgg cttcagggtg cggcgcgtct ttcgcgatca ccatcacgtc
      601 gaaccacagc aggccgcctt ccttcacgtt cgaaaacttg atctgatacg aacgcttggc
      661 ctcgtcggtg cggcggctcg caatgccgac gtcgcctgac cagcccaccg cgacgcagac
      721 gtcgttgttc gcgaggtcgt tgatgtagcc ggacgaattg aattgggtga tatacgggcg
      781 gacttttttc agcacttcga acgcggcctg atagtcggcg gggttggtgc tgttcggatc
      841 cttatgcatg tattgcagcg ttgcggcgaa tacgtcgacg gcctggtcga ggaaggacac
      901 gccgcagctt ttcagcttcg acagattggc cgggtcgaat acgggtgccc agctatccac
      961 cggcgcattc gcgccgaggg ctttctgcac ggcctgcacg ttgtagccga tgccatccgt
     1021 accgtatgcc cagggcacac cgtactggtt gcccggatcc gcgtcggcga tcatcttcat
     1081 caacacgggg tcgaggttca cgaggttcgg cagcttcgat ttgtcgagct tcaggtacac
     1141 gccggcctga atctgcttgg tcatgtagtt cgaggtcggc accacgatat cgtagccgga
     1201 gctgcccgcg agcagtttgg cttgcaacgt atcgtcgcta tcgtagttgt cgtacttgac
     1261 gtgaatgccg tcctgcttct cgaagttcgg aatcgtgtcc ttcgcgatgt agtccgatca
     1321 gttgtagaca ttcagttccg tgtcggccgc aagagccggc gtgactgaca acgccgcaaa
     1381 acccgtgaag gcgagaagcg cggcccccgc gaccgcatga cgaagatgac gtccagaatg
     1441 acacacgctc atggtttttc ccttgatgtg aagagccggc atgagcgcgt atcgtgggtg
     1501 tgctcatgcc ggaacgaact gccgttacga aattccgagt tgttgtgcgg tcgcgtcgat
     1561 ggctttcctg gccttcgaaa cgatttcgtc gatctccagc ttcgtgatca cgagcggcgg
     1621 cgagagcagc atccggtcgc cggtggcgcg catgatcagg ttgccgttga aacagaagtc
     1681 gcggcagatc gtgccgacgt caccaccgtt ggcgaaacgc ttgcgcgttt tcgggtcttc
     1741 tgcgagttgc agaccggcca ccaggcccgc gccggcgatt tcgccgatga tcgggtgatt
     1801 ggcgaaggtg tcgcgcagct tcttctggaa gtacggaccc gtgtcggtct tgacgcgctc
     1861 gacgatcttc tcgtcgcgca gcagcttcag attggcgagc gccacagccg cggctaccgg
     1921 gtggccggag taggtgaggc cgtgattgaa gtcgccattt tcgatgatcg ccgtggccac
     1981 gcggtcatgc agtccgaccg cgcccatcgg cacatagccg ctcgtcaggc ccttggccag
     2041 cgtgatcaga tcgggctcga agccgaagtg ctgatgcgcg aaccattcgc cagtccggcc
     2101 gaagccgccg atcacttcgt cggcgaccag caggatgtcg tatttgcggc agatgcgctg
     2161 gatttccggc cagtacgtcg aggccgggaa gatcacgccg ccggcgccct ggaaaggctc
     2221 accgataaac gcggcgacat tgtccgcgcc gatttcgagg atcttcgctt cgagttgttg
     2281 cgcacgggcc agcgcgaatt cttccggcgt ctgattgcct tctgcttcgc cgaagaaata
     2341 cggctgatcg atatgaacga tgttctcgac cttcgagggc atctgttcat gcatgtagcc
     2401 catgccaccc agcgtgccgc cggcgatcgt cgaaccgtga tagccgttct tgcgcgagat
     2461 gacgaatttc ttcgcgtgcc tgttttgcgt cgcccagtat tgatggacga tgcgcagcac
     2521 ggtgtcgttg ccttccgagc cgctattgca atagaagaag tggttgaacg gttcgggcgc
     2581 gagctccgcg agcaaggccg acagttcgat caccggcgga tgtgtcgtct tgaagaaagt
     2641 gttgtagtag ggcagttcct gcatctgctt gtacgcggcg tcggccagtt ccttgcggcc
     2701 ataaccgacg ttcacgcacc acaatccagc cataccgtcg ataaccttgt tgccttccga
     2761 atcccacagg tacacgcctt gcgctttgac gatcacgcgg ctgccggcgc gattgagcga
     2821 acccatgtca gaaaaaggat ggatgtgatg tgcggcgtcg agtgcccggt attcggcggt
     2881 gctgcgttgc tgctgtgtgg tttgtacgct tgcggccgtg ctggattgcg cggccggtcg
     2941 cacgtaagcg acttcttctg ttctatagct catactgcct ccagttttta tgcgtttgcg
     3001 ggcgacgtca tgaataaaga ccttggctct ggtcttagac gtgcagcagc aaatgccggc
     3061 gttcccacga actgatcacg cggaaaaatg cctcgtattc ggtttctttc aaagcaaggt
     3121 aggctttgac gaacttgtcg ccgagaactt ctgcaatcgg ctcgcatgag gccatcagcg
     3181 tcatgccttc ttcgagattg cgcggcaact gatacggcaa tgcgtagccg ttgctgagca
     3241 gcggctcggt cgcgtcgagt tgttgcgtca tgccgaggta gcccgcggcc agcgtcgcgg
     3301 cgatcgccag atacggattg cagtccacgc ccggaatgcg gttttcgatg cggcgcgcgg
     3361 ccggcgccga atgcggaatg cgaaagccca ccgttcggtt gtcgtaaccc catgcgacgt
     3421 tgatcggcgc ggccatgaaa cgcgacaggc ggcgatacga gttgatgtac ggcgcgaaga
     3481 tcggcatcag cgccggcgtg tacttctgca agccggcgat gtagctcgtg aacagcgaag
     3541 tcggcttgcc gtcggcgctg gtgaacacgt tgtggccgct ttcctcgtcg acgagacttt
     3601 gatgcatgtg catggccgag cccggttcgc cttccatcgg cttggccatg aacgtcgcgt
     3661 acatcttgtg gcgcagcgcc gcttcacgca ccgtgcgctt gaacaggaac acgctgtcgg
     3721 cgagcttcaa cggatcgccg tgcatgaagt tgatttccat ctgcgcggcg ccgacctcgt
     3781 gaatcagcgt gtcgacttcc agctcctgca cctcgcagta ctcgtagatg tcctcgaaga
     3841 gcggatcgaa ttcgttcacg gcttcaatcg aatacgcctg acgtccggtc tccgctcggc
     3901 ccgtgcggcc gatcggcggt tgcagcggca ggtcagggtc cttgttcatg tcgaccagat
     3961 agaactccag ctccggcgca atgacgggct tccagccctt ggccttgtag agttccagca
     4021 cctgacggag cacccggcgc ggcgaaatgg cgaccggtgt gccgtcgaag tg
//
LOCUS       DATASH010000501         2590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_870587,
            whole genome shotgun sequence.
ACCESSION   DATASH010000501 DATASH010000000
VERSION     DATASH010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_870587"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1501
                     /locus_tag="VHV99_13810"
     CDS             <1..1501
                     /locus_tag="VHV99_13810"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEX3636537.1"
                     /translation="SNGFLTGKSDCIVIGVFESQTLAGAALEIDAATKGLLTRIIKAG
                     DMDGKAGTTLFLHEVSGIGASRVLLVGLGKQDAFSQKAYGEAVRAAWRALLGTKIVQV
                     TFTLAQLPILERSADWGVRAAILALRELTYKFTQMKSKPDNAARALKRIVFSVNTGDE
                     KATKLAAKQGAALANGMDLTRDLGNLPSNVCTPTYLANTAKKLAKDWKLKVEVLGEKQ
                     CEALKMGSFLSVTAGSVEPAQFIVLQYHGGAAKAAPVVLVGKGVTFDTGGISLKPGEG
                     MDEMKYDMCGAGSVLGTLRAVAEMGLKINVVGIIPAVENMPSATATKPGDIVTTMKGL
                     TVEVLNTDAEGRLILCDALTYAERFKPAAVIDIATLTGACIIALGHHNSGLFSKNDAL
                     AGELLDASREASDPAWRLPLDDEYQDQLKSNFADLANIGGRPAGSVTAACFLSRFTEA
                     YPWVHLDIAGTAWKSGAAKGATGRPVPLLAQFLIDRAADGRAADGRAAQ"
     gene            1554..1970
                     /locus_tag="VHV99_13815"
     CDS             1554..1970
                     /locus_tag="VHV99_13815"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006750016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEX3636538.1"
                     /translation="MTRIDFHSNVGDSLLYACRLIRKAYQAGQPTIVLAEPERLRAFD
                     EQLWTFSPLDFVPHCMAGTPLAAQTPIVLASTLDDVPHHQVLLNLGVTVPAQFARFER
                     LLEVVGNAHEELAAGRERYRFYRDRGYALNNYKQGS"
     gene            2064..>2590
                     /locus_tag="VHV99_13820"
     CDS             2064..>2590
                     /locus_tag="VHV99_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2486 family protein"
                     /protein_id="HEX3636539.1"
                     /translation="MSDPNDSSIPVLHEILVPGNPAQARNASSGAAAPATQPVSDPET
                     GKEPVFAPEPSLEPVPVLAPESLHVREEPTLAPDPAHVPGQAHPADPAHPRKRSRAHH
                     GADHRHEHERALHEPAVVPSAGVFDRVEPHAPLEAGAIVPPDIAHEASAQSQSNLDAD
                     VIAERLRGRFAGFLTG"
BASE COUNT          470 a          832 c          851 g          437 t
ORIGIN      
        1 gtcaaacggt ttcctgaccg gaaaatccga ttgcatcgta atcggcgtgt tcgagtcgca
       61 aacgcttgcg ggcgccgcgc tggagatcga cgcggccacc aagggcctgc tgacccgcat
      121 catcaaggcc ggcgacatgg acggcaaggc gggcactacg ctgttcctgc acgaagtctc
      181 aggcatcggc gcttcgcgcg tgctgctggt cggcctcggc aagcaggatg ctttcagcca
      241 gaaagcctac ggtgaagccg ttcgcgctgc gtggcgcgca ctgctcggca ccaagatcgt
      301 ccaggtcacc ttcacgctcg cccagctacc gattctcgag cgctcggccg attggggcgt
      361 gcgcgccgcg atcctcgcac tgcgcgaact gacctacaag ttcacgcaga tgaagagcaa
      421 gccggacaac gcggcgcgcg cgctgaagcg catcgtcttc agcgtcaaca ccggcgacga
      481 gaaagccacc aagctcgcgg ccaagcaggg cgccgcgctc gccaacggta tggatctgac
      541 gcgcgatctc ggcaacctgc cgagcaacgt ctgcacgccg acttacctcg ccaataccgc
      601 gaagaagctc gccaaagact ggaagctgaa ggtcgaagtg ctcggcgaaa agcagtgcga
      661 agcgctgaag atgggctcgt tcctgtcggt cacggccggc tcggtcgaac cggctcagtt
      721 catcgtattg cagtaccacg gcggcgccgc gaaagccgcg ccggtggtgc tggtcggcaa
      781 gggcgtgacg ttcgacaccg gcggcatttc gctcaagccg ggcgaaggca tggacgagat
      841 gaagtacgac atgtgcggcg ccggttcggt gctgggcacg ttgcgcgcgg tcgccgaaat
      901 gggcctgaag atcaacgtgg tcggcattat tccggcggtt gagaacatgc cgtcggctac
      961 cgccaccaag ccgggcgaca tcgtcaccac catgaagggc ctgacggtcg aagtgctgaa
     1021 cacggacgcc gaaggccggc tgatcctgtg cgacgcgctg acctacgccg agcgcttcaa
     1081 gccggcagcg gtgatcgaca tcgccacgct gacgggtgcg tgcatcatcg cgctgggtca
     1141 tcacaatagc ggtttgttct cgaaaaacga cgcgctcgcg ggcgaactgc tcgacgcctc
     1201 gcgtgaagcg tccgaccccg cctggcgcct gccgctcgac gacgagtatc aggatcagct
     1261 caagtcgaac ttcgcggacc tcgccaacat cggcggccgt ccggcgggca gcgtgaccgc
     1321 ggcgtgcttc ctgtcgcgtt tcactgaagc gtatccgtgg gtgcatctgg acattgccgg
     1381 cactgcatgg aagagcggcg cggcgaaggg cgccaccggc cgcccggtgc cgttgctcgc
     1441 gcagttcctg atcgacaggg ccgctgacgg tcgggccgcg gacggtcgcg ctgcacaatg
     1501 acgcattgca gtaaggttgc ggccgtagcg gtagacgtaa agcggagccg ccgatgacga
     1561 gaatcgattt tcactcgaac gtcggcgatt cgctgctgta tgcatgccgt ctcattcgca
     1621 aggcgtatca ggccggccag ccgaccatcg tgctggccga gcccgagcgc ctgcgggctt
     1681 tcgacgaaca gttgtggacg ttctcaccgc tcgacttcgt gccgcactgc atggcgggca
     1741 cgccgctcgc cgcgcaaaca ccgatcgtgc tggcttcgac ccttgatgat gtgccgcacc
     1801 atcaggtact gctcaatctc ggcgtcacgg tgccggccca attcgcgcgc ttcgaaagat
     1861 tgctggaagt ggtgggtaac gcacacgagg agctcgcggc gggccgagaa cgctatcgtt
     1921 tctatcgcga tcgcggctat gctttgaaca actacaagca aggcagctag gtctcattcc
     1981 gcccgcgtcg cgtggtgttt tgtcggatgg attcgacgcg accgcggcct gcttcaggct
     2041 ggactcaaac acggagagtg cacgtgtccg atcctaacga tagttcgatc ccggttctgc
     2101 acgagattct cgtaccgggc aatcctgccc aggcgcgcaa tgcgtcgagc ggcgcggcgg
     2161 cgccggctac gcagccggtg tcggatcccg agaccgggaa agagccggtc ttcgcgccgg
     2221 aacccagtct cgagccggtg ccggtgcttg cgccggaatc cttgcacgtg cgggaggagc
     2281 cgacgctcgc gccagacccc gcgcatgtgc ccgggcaggc gcatccggct gaccccgcgc
     2341 atccgcgaaa acgctcgcgg gcacaccacg gcgccgacca ccggcatgaa catgagcggg
     2401 ctttgcatga accggccgtg gtgccgtcgg cgggcgtctt cgatcgggtg gagccgcatg
     2461 cgccgctcga agcaggcgcg atcgtgccgc cggatatcgc tcacgaagca tccgcacagt
     2521 cgcagtcgaa tctcgatgcg gacgtcatcg ccgaacgtct gcgtggacgg ttcgcgggtt
     2581 ttctgacggg
//
LOCUS       DATASH010000502         1630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_78328,
            whole genome shotgun sequence.
ACCESSION   DATASH010000502 DATASH010000000
VERSION     DATASH010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78328"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..1318
                     /gene="ftsY"
                     /locus_tag="VHV99_13825"
     CDS             134..1318
                     /gene="ftsY"
                     /locus_tag="VHV99_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006998949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEX3636540.1"
                     /translation="MPEAPASGTGPAVENGPTGAPAESIDRSARPANPTPAEAPDVAA
                     AGTPAPFTQPASPASATPELKPYWQGRTASQWKAPAETVEIVPPPEPEPNAKRSWLTR
                     LKTGLSKTSSGLTGIFVGAKIDEDLYEELETALLMSDAGVDATEYLLEALREKVRSER
                     LTDSMQVKAALRSLLADLLRPLEKSLMLGRAKPLVVMIAGVNGAGKTTSIGKLAKHLQ
                     RFDQSVLLAAGDTFRAAAREQLTVWGQRNNVTVVSQESGDAAAVIFDAVGAARARKID
                     VMMADTAGRLPTQLHLMEELRKVKRVIAKAQPDAPHEVLLVIDANTGQNALAQVKSFD
                     DALGLTGLIVTKLDGTAKGGILAAIARQRPIPVYFIGVGEKVEDLQPFSADEFADALL
                     GS"
     gene            complement(1380..>1630)
                     /locus_tag="VHV99_13830"
     CDS             complement(1380..>1630)
                     /locus_tag="VHV99_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEX3636541.1"
                     /translation="LNPHVYLDTVVLLGGIGARYGWPGNAWFAAGAMCSSVIWFTTLG
                     FGARLLQPLFEKDISWRVLDVIVGIVMWWIAAALILSR"
BASE COUNT          336 a          504 c          536 g          254 t
ORIGIN      
        1 gcacgccagt acgccggtga caccggctca gccggtggaa accgcgcgga aaagcgttgc
       61 ggcgtcggat cgggctttgg aatcgccatt gcgcgggacg gggtcgactg ctggaactgc
      121 tggtacgccg aggatgccgg aggcaccggc gagtgggacg gggcccgcgg ttgaaaacgg
      181 gccaaccggc gcgcccgcgg aatcgattga ccggtcggcg cgcccggcta acccgacgcc
      241 ggctgaagct cccgatgtag cggctgcagg aacgcccgcg cccttcacgc aaccggcctc
      301 gccagcgagc gcaacaccgg aactcaaacc ctattggcaa ggccgtacgg catcgcagtg
      361 gaaggcgccg gcagaaaccg tcgagatcgt gccgccgccg gagccggaac caaacgcaaa
      421 gcgttcatgg ctcacgcgcc tgaagacggg cttatcgaag acgagttcgg gcctgacggg
      481 gattttcgtc ggcgcgaaga tcgatgaaga tttgtacgag gaactcgaaa cggcgttgct
      541 gatgtcagat gccggtgtcg acgccaccga atacctgctt gaagcgctgc gtgaaaaggt
      601 gcgcagtgaa cggcttaccg attcgatgca ggtcaaggcc gcattgcggt cgctgctcgc
      661 ggatctgctg cggccgctcg agaagtcgct catgctcggt cgcgccaagc cgctcgtggt
      721 gatgattgcc ggcgtgaacg gcgcgggcaa gaccacgagc attggcaagc tggccaagca
      781 tttgcagcgc ttcgaccagt ccgtgttact ggccgctgga gacaccttcc gtgctgccgc
      841 gcgcgaacag ctcacggttt gggggcaacg caacaacgtg acggtggtgt cgcaggaaag
      901 cggcgatgca gccgccgtga tcttcgatgc agtcggcgcc gcgcgtgccc gcaagatcga
      961 cgtgatgatg gccgatacag ccggccgcct gcccacgcag cttcatctga tggaagaact
     1021 gcgcaaggtg aagcgcgtga ttgcgaaggc gcaaccggat gcaccgcacg aagtgctgct
     1081 ggtcatcgat gcaaacacgg gccagaacgc actcgcccaa gtgaagtctt tcgacgatgc
     1141 gctgggcttg acgggtttga tcgtgaccaa gctggacggc acggcgaagg gcggcattct
     1201 cgcggccatc gcccggcagc ggccgattcc cgtgtacttc attggcgtgg gcgagaaggt
     1261 ggaggacttg cagcccttca gcgccgacga gttcgcggac gcattgcttg gaagttagct
     1321 tggaagtcaa gccgatgccg cggcttgggc gttcagccag accgcggccg cgcagcgcac
     1381 taccgggaca gaattagcgc tgccgcaatc caccacatca caatccccac gatcacatcg
     1441 agcactctcc acgagatgtc tttctcgaac agaggctgga gcaagcgcgc accgaacccg
     1501 agcgtggtga accagataac cgacgaacac atcgccccgg ctgcgaacca cgcgttcccc
     1561 ggccagccgt atcgcgcgcc aatcccaccc agcaacacaa cggtgtcgag ataaacgtgc
     1621 ggattgagca
//
LOCUS       DATASH010000503         5640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6210182,
            whole genome shotgun sequence.
ACCESSION   DATASH010000503 DATASH010000000
VERSION     DATASH010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6210182"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(344..1297)
                     /gene="tal"
                     /locus_tag="VHV99_13835"
     CDS             complement(344..1297)
                     /gene="tal"
                     /locus_tag="VHV99_13835"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEX3636542.1"
                     /translation="MTTALDQLKQYTTVVADTGDFQQLAQYKPRDATTNPSLILKAVQ
                     KDDYKPLLEKTVKAHASKPVAAIIDQLLIAFGTEILKIIPGRVSTEVDARLSFDTEAS
                     IAKGRELIALYKEHGIGRERVLIKLASTWEGVRAAEVLQKEGIHCNMTLLFSLAQAAA
                     CAEAGAQLISPFVGRIYDWYKKNAGSAWDEAKDGGANDPGVKSVRRIYAYYKKFGYKT
                     EVMGASFRTPGQILELAGCDLLTISPDLLQKLQESTEKVERKLSPEIATGADIERVPV
                     DESSFRFLVNDEAMATEKLAEGIRAFAADAVKLEKLIEALR"
     gene            complement(1775..2995)
                     /locus_tag="VHV99_13840"
     CDS             complement(1775..2995)
                     /locus_tag="VHV99_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HEX3636543.1"
                     /translation="MSQSSAPDLSPADTPPGRFNPIADTSLSAIVAGFVAMMTGYTSS
                     LVLMFQAGQAAHLTDAQISSWIWALSIGMAVCTLGLSLRFRAPIVIAWSTPGAALLVW
                     SLPHVSYPEAIGAFVVCAVLLTVVGLTGWFDTLMKKIPAGIASALLAGILFEIGIEIF
                     RAAQFQTALVLTMFFTYLVVKRLAPRYAIVTTLIVGTAAAGALGLLDFSRFHVALAHP
                     VFTVPAFSVAASISIGIPLFVVAMASQNVPGIAVLRADGYSTPSAPLISTTGIASLLL
                     APFGSHGINLAAITAAICTGPEAHENRDKRYTAAVWCGIFYLIAGIFGATIAALFAAL
                     PKALVVSVAALALFGSIMSGLANAMQDLKQREAALVTFMVTASGLTLLSIGSAFWGLV
                     AGVLTQVVLNARRA"
     gene            complement(3036..4211)
                     /locus_tag="VHV99_13845"
     CDS             complement(3036..4211)
                     /locus_tag="VHV99_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX3636544.1"
                     /translation="MLIDFFYSLRAAKLPVSVKEYLTLLEALKANVIAPSLDEFYYLA
                     RLTLIKDEQYFDKFDQAFGAYFNGVAKASELAFDVPLEWLKKKLQRDLSPEEKAQIEA
                     MGGLDKLMERLKELFDEQKERHEGGNRWIGTGGTSPFGNGGYNPEGVRIGGDAAGNRT
                     AVKVWEARAYRDYDDQVEIGTRNIKVALRRLRRFAREGAAEELDLPDTIRSTAANAGW
                     LDLKMVPERHNKVKVLMLLDVGGSMDDHIKRTEELFSAAKAEFKHLEFYYFHNCVYDF
                     LWKNNRRRHAERMPTWDVLHTFTPDYKLIFVGDATMSPYEVLQPGGSVEYNNAEAGAV
                     WLRRLADHFPHHAWLNPEPEGLWAYRQSVSAIREVLGHRMYPLTLAGLETAMRMLSK"
     gene            complement(4290..5132)
                     /locus_tag="VHV99_13850"
     CDS             complement(4290..5132)
                     /locus_tag="VHV99_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX3636545.1"
                     /translation="MRFEGSSQYVATDDLKLAVNAAMTLKRPLLIKGEPGTGKTMLAE
                     EVAAALGMPLLQWHIKSTTKAQQGLYEYDAVSRLRDSQLGDERVKDIRNYIVKGVLWQ
                     AFESDEQTVLLIDEIDKADIEFPNDLLRELDRMEFYVYETHELIRAKQRPLVIITSNN
                     EKELPDAFLRRCFFHYIKFPDPVTMQQIVEVHYPGIKKELLAAAMQSFFELRNVAGLK
                     KKPSTSELLDWLKLLLAEDIPPEALRSSDQKQIIPPLHGALLKNEQDVSLFERLIFMN
                     RNNR"
     gene            5379..>5640
                     /locus_tag="VHV99_13855"
     CDS             5379..>5640
                     /locus_tag="VHV99_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636546.1"
                     /translation="MALAVALRCPDAPRPRTVDVRRGVAVQLARFFCLRGYPYKKNGA
                     GRCLLGARSPHARLHAMNKFVGKHVVIAALSVLAGYTASVQAA"
BASE COUNT         1000 a         1729 c         1810 g         1101 t
ORIGIN      
        1 cttcttcaca gcggccgttg aaaaatacgt aaggctgaac gtacatgatc gtctcccgga
       61 atagggttag cggcgccgcg ttccttcggc aaacggcaac gagggagcag gcgtgcagac
      121 gattgtagtc gtccgttggg gcagcgattg tggcgttatt tcgtaggagg cgaggcgtgg
      181 gcgcctatcg gacgtattgg atatagcgga cgtgcgcctg taaacggcac gcggcccaga
      241 accggagggc tcggggccgc gtattcagca cctgcgcaac cacatgtgca atcaaatccg
      301 cgatcacacg tgcgatcgcg tgcgtaagcg ccgaaggcga agcttaacgc agtgcttcga
      361 tcagcttttc cagtttgacg gcgtccgcgg caaacgcgcg gataccttca gcgagctttt
      421 cagtcgccat tgcttcgtcg ttgacgagga aacggaacga ggattcgtcg accggcacgc
      481 gctcgatgtc cgcacccgtg gcgatctcgg gcgacagctt gcgctcgacc ttctcggtgc
      541 tctcctgcag cttttgcagc agatccgggc tgatggtcag cagatcgcag cccgccagtt
      601 ccagaatctg gcccggcgtg cggaagcttg cgcccatcac ctcggtcttg tagccgaact
      661 ttttgtagta cgcgtaaatg cggcgcaccg atttgacgcc cggatcgttg gcgccgccgt
      721 ctttcgcttc gtcccaggcg ctgccggcgt tcttcttgta ccagtcgtag atacggccga
      781 cgaacggtga gatcagttgc gcgcccgctt cggcgcaggc agcggcttgc gcgagcgaga
      841 acagcagcgt catattgcag tggatgcctt ccttctgcag cacttcggcg gcgcggacgc
      901 cttcccacgt ggaggccagc ttgatcagca cgcgttcgcg gccgatgcca tgctctttgt
      961 acagggcgat cagctcgcgg cccttggcga tcgacgcctc ggtgtcgaac gacagacgcg
     1021 cgtccacctc ggtcgacaca cggcccggga tgatcttgag aatttcggtg ccgaaggcga
     1081 tcagcaactg atcgatgatc gcggcgaccg gcttcgacgc gtgtgctttc acggtctttt
     1141 cgagcagcgg cttgtagtcg tctttctgaa cggccttcag aatcagcgac ggattggtgg
     1201 tcgcgtcccg cggcttgtac tgggcaagct gctggaagtc gccggtatcg gccacgacgg
     1261 tggtgtattg cttgagttga tcgagtgcgg ttgtcatgtt cgagccttca tggcgcgcgt
     1321 gcgccgtggt tcaggagaaa tgagattgcc accggacaga acagacaaaa tcgttccggc
     1381 ggccgcgggt cacgctgcag cgtctgctcg ccatgttgcc ggggaaatct ggcggaaggc
     1441 gctgccggat gcttctattc tatggcggat cgcctgatgc agtattgaca cttgccccgg
     1501 gcgatccgtt cacggggatt cggggggacg gttttctgtg ctgaatccgg cccccttccg
     1561 atgtcgattt tcggcgccga tttgtcgcct ggccaacttc cgatggattt tccacgccga
     1621 tctaccgcct gcccatcctc cgatggttga ttctctgcgc cgatctaccg cctgcccgtc
     1681 ttccgacgct gattctcggc gccgatctgc cgcctggcta tcttccgacg ctgattctcc
     1741 gcgccgaact gccaccagcc cactgtctcc ggcttcaggc gcgccgggcg ttcagcacaa
     1801 cttgcgtcag cacgcccgcc accaggcccc agaacgccga gccgatcgac agcagcgtca
     1861 gacccgaggc cgtcaccatg aacgtcacca gcgccgcttc gcgttgcttc aggtcctgca
     1921 tggcgttggc gaggccgctc atgatcgagc cgaacagcgc cagcgcggcg accgagacca
     1981 ccagcgcctt cggcaaggcg gcaaacagcg cggcgatggt cgcgccgaaa atcccggcaa
     2041 tcaggtagaa aatgccgcac cacaccgctg ccgtgtagcg cttgtcgcgg ttttcgtgcg
     2101 cttcggggcc ggtacagatc gccgccgtaa tcgccgcaag attgatgccg tgcgagccga
     2161 acggtgcgag cagcaacgag gcgatgccgg tggtcgaaat cagcggcgcg gagggcgtgc
     2221 tgtagccgtc cgcgcgcagc acggcgatgc ccggcacgtt ctgcgaggcc atcgcgacca
     2281 cgaacagcgg aatgccgatg ctgatgctcg ccgcaaccga aaacgccggc accgtgaaca
     2341 ccggatgggc gagcgcgacg tgaaagcggc tgaagtcgag cagtccgagc gcgccggcgg
     2401 cggccgtgcc gacgatcagc gtcgtcacga tcgcatagcg cggcgcgagg cgtttgacga
     2461 ccaggtaggt gaagaacatc gttagcacga gcgccgtctg gaactgcgcg gcgcggaaaa
     2521 tctcgatgcc gatctcgaac agaatgcccg ccagcaaagc cgaggcgatg cccgccggga
     2581 ttttcttcat cagcgtgtcg aaccagccgg tcaggccgac caccgtcaac agcaccgcgc
     2641 agacgacaaa cgcgccgatc gcttcggggt aggacacatg cggcagcgac cacaccagca
     2701 gcgcggcgcc aggcgtcgac catgcaatca cgatcggcgc gcgaaagcgc agcgagaggc
     2761 cgagcgtgca cacggccata ccgatcgaga gcgcccagat ccacgaagaa atctgcgcat
     2821 cggtgagatg cgcggcctgg ccggcctgga acatcagcac gagcgagctg gtatagcccg
     2881 tcatcatcgc gacgaagccg gcgacgatcg ccgacagcga ggtgtcggca atcgggttga
     2941 aacgcccggg cggcgtgtcg gcaggggaca aatcgggcgc tgaagactga ctcatacaga
     3001 tgctttctta tagttggatg aaaaacgcga gcggattatt tgctcaacat gcgcatcgcg
     3061 gtttcgagtc cggcgagcgt aagcggatac atgcgatggc cgagcacttc gcggatcgcg
     3121 ctgaccgact gccggtacgc ccataaacct tccggctccg gattgagcca cgcgtgatgc
     3181 gggaaatggt cggccaggcg gcgcagccac acggcgccgg cttctgcgtt gttgtattcg
     3241 accgagccgc ccggttgcag cacttcgtac gggctcatgg tcgcgtcgcc gacgaagatc
     3301 agcttgtagt cgggcgtgaa cgtgtgcagc acgtcccacg tcggcatgcg ctcggcgtga
     3361 cggcggcggt tgttcttcca cagaaagtcg tacacgcagt tgtggaagta ataaaactcc
     3421 agatgcttga actctgcctt ggcggcggaa aagagctctt cggtgcgctt gatgtgatcg
     3481 tccatcgagc cgcccacgtc cagcagcatc agcactttca ccttgttgtg gcgctccggc
     3541 accatcttca gatcgagcca gccggcgttc gccgcggtgc tgcggatcgt gtcgggcaga
     3601 tcgagctctt cggcggctcc ttcgcgagcg aaacggcgca agcggcgcag cgcgaccttg
     3661 atattgcgcg tgccgatttc gacctggtcg tcgtaatcgc gataggctcg cgcctcccac
     3721 accttcaccg ccgtgcgatt gcccgccgcg tcgccgccga tgcgcacgcc ctccgggtta
     3781 tagccgccgt tgccgaacgg cgaggtgccg cccgtgccga tccatctgtt gccgccttcg
     3841 tggcgctctt tctgttcgtc gaacagttcc ttcaggcgct ccatcagctt gtcgaggccg
     3901 cccatggctt cgatctgcgc tttttcttcc ggtgacagat cgcgttgcag ttttttcttc
     3961 agccactcga gcggcacatc gaaggcaagc tccgaggctt tcgcgacgcc gttgaaatac
     4021 gcgccgaatg cctggtcgaa cttgtcgaaa tactgctcgt ccttgatcag cgtgaggcgc
     4081 gcgagatagt agaactcgtc gagcgacggc gcgatcacat tggctttcag cgcctcgagc
     4141 agcgtcaggt attccttcac cgacaccggc agtttggcgg cgcgcagcga gtagaagaag
     4201 tcgatcagca tgctgtgtcc tcggcggggt ccggcgtgcg gcgcggcgcc ttcatcaacg
     4261 ggtattcgcg gcgagtcatc cgcggtgaat caacggttgt tgcggttcat gaagatcaac
     4321 cgctcgaaca ggctcacgtc ctgctcgttc ttcaggagcg cgccgtgcag cggcgggatg
     4381 atctgcttct ggtcggacga gcgcagcgct tcgggcggaa tatcttccgc gagcagcagc
     4441 ttcagccagt cgagcagttc cgacgtggac ggtttcttct tcagaccggc aacgttgcgc
     4501 aactcgaaga agctctgcat cgccgcggcg agcagttcct tcttgatgcc ggggtagtgc
     4561 acctcgacga tctgctgcat cgtcaccgga tcggggaatt tgatgtaatg gaagaagcag
     4621 cggcgcagga acgcgtcggg cagttccttc tcattgttcg acgtgatgat cacgagcgga
     4681 cgctgtttcg cgcggatcaa ctcgtgggtc tcatagacgt agaactccat gcggtcgagt
     4741 tcgcgcagca ggtcgttcgg gaattcgatg tcggccttgt cgatctcgtc gatcagcagc
     4801 acggtttgct cgtccgattc gaacgcctgc cacagcacgc ccttgacgat gtaattgcgg
     4861 atgtccttga cgcgttcgtc gccgagctgc gaatcgcgca ggcgcgacac cgcgtcgtac
     4921 tcgtacaggc cttgctgggc cttggtggtg gacttgatat gccactgcag cagcggcatg
     4981 ccgagcgcgg cggcgacctc ttcggccagc atggttttgc ccgtgccggg ttcgcccttg
     5041 atcagcaacg ggcgcttcag cgtcatcgcg gcgttgaccg cgagcttgag gtcgtcggtg
     5101 gcgacgtact gcgatgagcc ttcgaaacgc atggcgagat gctctttttt gggaaaaaac
     5161 ccagtataag tcagaaggcc tgttggcccg aaacgggctc gcgtaaagtg tcacccgcgg
     5221 tgtcggacgc tgcagcgggg cgcacacagg gcgttcgcga gcggcccgta cgcttgccca
     5281 gcgccgcccg gtgggcgggc gcgccgggtg gcgcgggccc aagcggccgg attgcgcagt
     5341 gagcgccagg cgacgtgggc gaagcgcggc ggagcagaat ggcgctagcc gtggctttgc
     5401 ggtgcccgga tgcaccgcgg ccccgcacgg tggacgttcg gcgcggcgtc gcggtacaat
     5461 tagcccgatt tttttgcctg cgtggctacc cttataagaa gaacggcgcg ggccgttgcc
     5521 ttttgggcgc ccgttcccct catgccaggt tacatgctat gaataaattc gtcggcaaac
     5581 acgtcgtgat cgcagcgctg tcggtgctcg cgggctatac ggccagtgtg caggcagcgg
//
LOCUS       DATASH010000504        12179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4825813,
            whole genome shotgun sequence.
ACCESSION   DATASH010000504 DATASH010000000
VERSION     DATASH010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 12179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12179
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4825813"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..1034)
                     /locus_tag="VHV99_13860"
     CDS             complement(111..1034)
                     /locus_tag="VHV99_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HEX3636547.1"
                     /translation="MWFKNLQLHRLPAPWSVTPEQMEKWLAPHAFAPGNSVEMQSHGW
                     ASPRDNDSLVYSLNGQMLLVFRTEKKLLPASVVTQFTKARALELEEQQGFKPGRKQMR
                     EIKEQVTDELLPRAFSIRRDTRVWIDTRNGWLVIDAGSQAVADEVRGLLVKSIDQLPL
                     GTVRVTQSPVAAMTGWLLSGEGPAGFTLDQDTELRSPTEGNATVRYVGHTLDAEDMRR
                     HIEAGKQCMRLAMTWNDRVSFVLTPSLTIKRIAPLDVLKEASDPTAQNDDERFDSDVT
                     LMTAELDRMLNDLLDALGGEHGEAMPQAAAA"
     gene            1441..1761
                     /locus_tag="VHV99_13865"
     CDS             1441..1761
                     /locus_tag="VHV99_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636548.1"
                     /translation="MADETAPWQMVEYEGFEIHVAPFPKNPPDETKPSGVTDNRYTYV
                     GYVCHPGADPTIPGHAVPFHADGEESFTGKDEALYEGVHVGRSIVDGTHPDLTVLPLV
                     TGGV"
     gene            complement(1800..2645)
                     /locus_tag="VHV99_13870"
     CDS             complement(1800..2645)
                     /locus_tag="VHV99_13870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006409051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEX3636549.1"
                     /translation="MFSIIIPTWNNLPYLRLVIESLRRHSTHAHQVIVHVNDGSDGTL
                     AWVRDEGIEHTASPGNIGICHAVNLAAARATQDYIVYMNDDMYCCPGWDDALVKRLAQ
                     MPADNLFMLSGTMIEPVDSGNPCVVVQNFGRDVENFRADELVAAAPGLVRADWRGATW
                     PPTLVHRDWWYKVGGYSSELSPGMSSDNDFSMKLWDAGCRVFLGAGDSLVYHFQQKST
                     GKVVKNDGRRQFLNKWGMTQATFDRYFLRRGTAVQGSQAVSEPERTGRLRRALLKSRI
                     KRAFS"
     gene            3130..4428
                     /locus_tag="VHV99_13875"
     CDS             3130..4428
                     /locus_tag="VHV99_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX3636550.1"
                     /translation="MIFAPSLVWLSTALLFFAPAVNLVWRGGTGYCFFAILALALGAA
                     VANRRMPGYFSGLRTYRWFTIGMLAFVVTIAAQQALFGYWLPRQFDALSRFVLAVPVF
                     LLLRQLPSRHLRVIGWGCAAGALAVGAWALMDQPAGGWTDANRLNNSYTNAIPFGDTA
                     LLLGFLSVFTLGWDKPRDWLVFGLRLLALVGGGYASYLSGSRGGWLAVPVFVVLLGMQ
                     YQWYAHKKRLLITTLAIVIGAGALLSTERVQKRLGEVTNDVSMMHQGEDYTSVGLRFQ
                     LWNASGLIFMHHPVYGVGKGRLVDELGLLAKRGEVKAEIVNERAHSDFFSTLAEMGAI
                     GVLCLLLFYYGISVYFWRHRLSSDPAIRAASYAGLAVATSTVIFGLTIDVLVPIMVTV
                     LLALLVATFLAVIDSRKRELATAQPAAARAARQTTTGVKA"
     gene            complement(4592..5671)
                     /locus_tag="VHV99_13880"
     CDS             complement(4592..5671)
                     /locus_tag="VHV99_13880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX3636551.1"
                     /translation="MSDPTRRPVTDIVLTAQALKNSGGAERYTRDVIAGLHRMGLRPT
                     LFAREIDRTLPEAAWIDAQPLNVRWAPRKLRNLAFDWRLKQRLKRHRPACVFSINHST
                     HADVALCGGTHPGSLEAAGRTARRSDEWQIDMERRVYSQARSIVAHSQLMSRELQRFY
                     GVPAERIDVLYPPVDTARFQPLNDAERAAVRRQFGLPEDRVIFVFSSTSHERKGYPLL
                     EAFFAQTTLPVCLAVAGRPVPKTSDTIRYVGYCKEIEKLFAAADFTVVASAYEPFGLV
                     GVESVMCGTPLVIADNVGSAETVTGAAKIEFSRQSAGSFERAIQAAVERVRDGDARIA
                     EPLRSLVYDPGVDAHVAALYELFTR"
     gene            5826..6851
                     /locus_tag="VHV99_13885"
     CDS             5826..6851
                     /locus_tag="VHV99_13885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018431562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3636552.1"
                     /translation="MTDSPSTSPPSATPDTDRPLVSILLIAYNQEKQIADAVQSALAQ
                     TYTPLEIIISDDASSDATFAAIEAAIAGYRGPHKVIARRNASNRGISAHLSQIAQLAE
                     GELLFVAAGDDMSAPNRCERVVDFWLAHDRRPDLIATDLADMDEGGNVHERMSPTELD
                     TYRSFDDWLAGRPWLIGAAHTWSRRLFERFGPMLPGAAAEDQIMVLRAILSGGAASLR
                     EPLVRYRRGGLSRKRRYGSVDELIARMRQSNRHGLAELAQLQRDAEIGGVSERMRAAL
                     ASKLAREQFIHAMLEAGGLAERIGLLTRSPNVKLGLRIRMFLYATCPAVYAPGIWLKR
                     IVRKNGR"
     gene            complement(6873..7916)
                     /locus_tag="VHV99_13890"
     CDS             complement(6873..7916)
                     /locus_tag="VHV99_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX3636553.1"
                     /translation="MHHDDPTSKPLVSIALATYNGRTYLPELLASLEVQSWPNLEVVV
                     SDDASTDGTPELLAAYSGRVQVRLIASGERVGIVRNFERALAGCRGEYVALADQDDVW
                     APEKLADLMHELQAVEKARGLATPALAFCDIELVDATLCRLSKSLFDITAKSRRAERL
                     RDFLLSNHIPGCAMLVNRAALQRALPFPAGIVMHDWWLAMVVASFGEIRHVAAPHLKY
                     RQHESNAVGASATNQRSAAGARDECKLAAAERRGASKRRQIDGIADQIQLFGQRFGAE
                     LPLDARDDMNAFSRGIENWRGALEFVVFSHTGETFLRAMKMMRRLRKSLLSFGVEAQQ
                     PFGRRLRGRRSAS"
     gene            complement(8172..9965)
                     /gene="msbA"
                     /locus_tag="VHV99_13895"
     CDS             complement(8172..9965)
                     /gene="msbA"
                     /locus_tag="VHV99_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HEX3636554.1"
                     /translation="MSAKPTLSKPIGSGEASSPAVVFRRLWPYIKPLMWVVVGAVVAM
                     AVSSATDAAIPALLKPLLDKGFGAHASDNAKWFVPVAVIGLALIRGVSQYASGYLLAY
                     VSNKILLELRLKMFNRMIHTSVAFFQRETASTVINAIVFEVNQILNVLLSVMVTLVRD
                     SLTVVFLLGYLFYLNWRLTLIVAVLLPGIGWLVGKINRRLRRLNREHQMLTNELSYIV
                     EETVGGYKVVKVHNGEQYETDRFTAMSKRLRGYAMRMTVSGGLAQPLTQFLSSIALAV
                     VITIAVVQSSSDQTTVGGFVAFVTSMLLIISPLKHLMDVNQPLQRGMTACELIFGLID
                     EPSEPEGGGKPLDRARGEVEFRDVSFTYGSNATHNRHTLDRVSFKVAPGEMVALAGPS
                     GSGKTTLVNLLPRFFDPTGGEILVDGVALPEYDLHALRSQIAMVSQDVVLFNDTVANN
                     VAYGQTADPEKVKAALRAANLWDTVEAMPNSIDTLVGDNGMMLSGGQRQRLAIARAIY
                     KDAPILILDEATSALDSESERHVQAALETLMKGRTTLVIAHRLSTIERADRILVLEAG
                     RIVERGSHRELLTQDGLYAHLHRIQFQQDAA"
     gene            10188..10955
                     /locus_tag="VHV99_13900"
     CDS             10188..10955
                     /locus_tag="VHV99_13900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX3636555.1"
                     /translation="MQETTLGVAIITKNAAARLAECLQAVAFADQIVVIDGGSTDGTA
                     EIARAHGARVLQQTDWPGFGPQKNRAVDALTTSWILSIDADEIVSPELASAIRAALTA
                     PTADVYAVDRLSSFCGHWIHHSGWYPDWIPRLFKRGAARFSDDLVHERLVFDTPSQRL
                     TGKLMHYSYEDFEGVLRKLDAYSTAGAQQRHAAGKRGSFGKALGRGAWAFVRTYVLRR
                     GFLDGRAGFMIALFNAEPVYYRFVKLARLGEQARRQD"
     gene            complement(11142..>12179)
                     /locus_tag="VHV99_13905"
     CDS             complement(11142..>12179)
                     /locus_tag="VHV99_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEX3636556.1"
                     /translation="NAIEEIIRAREEGPFIDIFDFCNRVDRRIVNRRTVEALIRAGAF
                     DTLHANRAQLIASVSLAMEAAEQASANAMQAGLFDMGDAPSQGHELVDEPEWPEKKKL
                     QEEKAALGFYLSGHLFDAYKDEVRRFVRQKIGELKEGRDKLVAGVIASLRTQMTQRGK
                     MLIALLDDGTGQCEVTVFNETFEAHKQLFKEDELLVVQGQARNDAFTGGIRFTVDTVM
                     DLGRARCRYAEAVKVQMNGNADALALRRVLEAHSAGKDEPQAAPAPAASSRGNGGGAG
                     AGRNGGGYGGGGQRQAVQIPNGLAVQVVYRSENAQGEMRLGDAWRVKPTDELLAALRG
                     EFAGSSIEIVY"
BASE COUNT         2209 a         3914 c         3801 g         2255 t
ORIGIN      
        1 cgtgtccgcg catgtcgacg cgttcatgga gagtcggcgc gtcctgggag gtagcggccc
       61 cgtgcatggc cgcatcgtcg cggccacata tcgaggcgtg tgcgttggac tcaggcagcc
      121 gcggcttgcg gcatcgcctc gccgtgctcg ccgcccaacg cgtcgagcag atcgttcagc
      181 atgcgatcga gttcggcggt catcagcgtg acgtcggaat cgaaacgctc atcgtcgttc
      241 tgcgcggtcg ggtcgcttgc ttccttgagg acgtcgagcg gtgcgatgcg cttgatcgtg
      301 agcgagggcg tcagcacgaa cgacacgcga tcgttccacg tcatcgccag acgcatgcat
      361 tgcttgccgg cttcgatatg acggcgcata tcttcggcat ccaaagtatg tccgacatag
      421 cgcaccgtcg cgttgccttc ggtcggcgag cgcaactcgg tgtcctggtc gagcgtgaag
      481 cccgcggggc cttcgccgga aagcagccag ccggtcatcg ccgcaaccgg cgattgcgtg
      541 acgcgcacgg tgccgagcgg caattgatcg atcgacttca ccagcagacc gcgcacttcg
      601 tcggcgaccg cttgcgagcc cgcatcgatc acgagccagc cgttcctggt atcgatccac
      661 acacgcgtat cgcggcgaat gctgaaggcg cgcggcagca gttcgtcggt gacctgctcc
      721 ttgatctcgc gcatctgctt gcggccgggt ttgaagccct gttgctcttc gagttcgagc
      781 gcgcgtgcct tggtgaattg cgtgacgacc gaagccggca gcagcttctt ctcggtgcgg
      841 aacaccagca gcatctgacc attgagcgaa tacaccagcg agtcgttatc gcgcggcgac
      901 gcccaaccat ggctttgcat ctcgacgctg ttgccgggcg cgaacgcgtg cggcgcgagc
      961 catttctcca tttgttcagg ggtgacggac cacggagcgg gaaggcggtg caactggagg
     1021 tttttgaacc acatgggcgg ggtcttgtac gggcaaagcg cgtcattcta tatgacgcgg
     1081 cgaagatggc gccgtcagaa gcctaagcgc tcgctgcggc gaagcgctgc cttcgcaaca
     1141 gccattttca aatgaaagcc gagatccttt tggcgacaga ccgggttcca cgcgcacttc
     1201 accggacagt tccagaactt gaaaggcaat ggccaacacg tcgatttcca gcctttcgac
     1261 ctttatcgcg tgaaggacgg cacatcgtcg aaaactggcg catctaggac aagctgacgc
     1321 tgctcgaccc gctcgggtgc gatcaagccg tagtggttta gccgcatcag tcctggggcg
     1381 cgctttatgt tcaaatcacc taaacttttt aagaaacgcg cgacttggac ggaggcggcc
     1441 atggccgatg aaaccgcacc atggcagatg gtggagtacg agggcttcga aattcatgtg
     1501 gcgcccttcc cgaaaaaccc gcccgacgaa acgaagccgt caggcgtgac ggataaccgc
     1561 tacacgtatg tcggctatgt gtgtcatcca ggcgccgacc cgaccatccc cgggcatgcg
     1621 gtgccgttcc atgcggacgg cgaggagagc ttcaccggca aggatgaagc actctacgaa
     1681 ggggtgcacg tggggcgtag catcgtcgac ggcacgcacc cggatctgac ggtgctgccg
     1741 ctggtgacgg gcggcgtgtg acgtacgcaa aagcgcgcgc cgcgcacgtg cccgtagctt
     1801 tagctgaacg cccgcttgat gcgcgacttg agcagcgcgc ggcgcaagcg gccggtccgt
     1861 tcaggctcgc tgactgcctg gctgccttgc accgccgtgc cgcgccgcag aaaataacga
     1921 tcgaaagtgg cttgcgtcat gccccacttg ttcaggaact ggcggcgccc atcgttcttg
     1981 acgaccttgc cggtgctctt ctgctggaag tgataaacga gactgtcgcc ggcgccgaga
     2041 aacacccggc aaccggcgtc ccacagcttc atcgagaagt cgttgtcgct gctcatgccg
     2101 gggctcaatt cgctgctata gcccccaacc ttgtaccacc agtcgcgatg caccagcgtg
     2161 ggcggccacg tggcgccgcg ccagtcggcg cgcacgagtc ctggcgcggc ggccaccagt
     2221 tcgtcggcgc ggaaattttc cacatcgcgg ccgaagttct gcacgacgac gcacggattg
     2281 ccggagtcga ccggctcgat catcgtgccg gacagcatga acagattgtc ggccggcatc
     2341 tgcgcgaggc gcttgacgag cgcgtcgtcc cagccagggc agcagtacat gtcgtcgttc
     2401 atgtagacga tgtagtcctg cgtggcgcgc gccgccgcga gattgaccgc atgacagatg
     2461 ccgatattgc cgggcgacgc ggtatgttcg ataccctcgt cgcgcaccca cgccagggtg
     2521 ccgtccgagc cgtcgttcac gtgcacgatg acctgatgcg cgtgcgtgga atgacgccgc
     2581 aagctctcga tcacgagccg caggtaaggc agattgttcc aggtcgggat gatgattgag
     2641 aacatcgatt cggtccgttg agcttgccgt cggcgcgagc gcggttcaac tccgcattgg
     2701 caatccggcg tggggcagtg caatgcaatt aaatggccac cgtgccggcg acatcaccgg
     2761 acgcggcgtt gaagaatcgc tgtcggcgcg agtgtctgcg gacgccgtgg gaacctgacg
     2821 ccgcacaggc agccagacag catcgcccat cagtttcgcc ggggattgta ccgccgccgc
     2881 ccgctcatac gacaaatacc atggtccgca aaccttgatg gccgtctttg agcaagacct
     2941 tccgcacaag tcggcaccgg ttgcggcagc ttcgtcgaag cacaccttac agtgtgctcc
     3001 agcaagcatt ttgcaagcga acgcacggtt ataatcgcga tcgctctacc gtcttaaaaa
     3061 atgcacccaa gggtccggcc atagcaattc caccaccgga acggtagcgt tcttacatat
     3121 tgttgcccta tgatttttgc tcccagtctg gtctggctca gcacagccct gctgtttttc
     3181 gcgcccgcgg tcaatctcgt gtggcgcggc ggcaccggct actgcttctt cgcaattctc
     3241 gcgctggcgc ttggcgccgc cgtggccaac cggcgcatgc cgggctattt ctccggcctg
     3301 cgcacctacc gttggttcac gatcggcatg ctggccttcg tcgtaaccat cgccgcccaa
     3361 caggcgctgt tcggctactg gctgccgcgc caattcgacg cgttgtcgcg cttcgtgctt
     3421 gccgtgccgg tctttctgct gctgcggcag ttgccttcca ggcatctgcg cgtgatcggc
     3481 tggggctgtg ccgccggcgc gttggcggtg ggcgcctggg cgcttatgga tcaacccgcc
     3541 ggcggctgga cggatgcaaa tcgtctgaat aattcctata cgaatgcgat tccattcggc
     3601 gacactgcgc tgctgcttgg ttttctgtcg gttttcactt tgggctggga caagccacgt
     3661 gactggctcg tgttcgggct gcgcttgctg gcgctggtgg gcggcggcta cgcatcctat
     3721 ctgtcgggca gccgaggcgg ctggctggcc gtgcccgtgt tcgtcgtgct gctcggcatg
     3781 cagtatcagt ggtacgcgca caagaagcgt ctgctgatca ccacgctggc gatcgtgatc
     3841 ggcgcgggtg cgctgctgtc gaccgagcgg gttcagaagc gcctcggcga agtgacgaac
     3901 gacgtttcga tgatgcatca gggcgaagac tacacgtcgg tcggcctgcg cttccagttg
     3961 tggaatgcgt ccggcctgat ctttatgcat catccggtct acggcgtcgg caaggggcgt
     4021 ctggtggacg agctgggact gctcgcgaag cgtggcgaag tgaaggcgga gatcgtcaac
     4081 gagcgcgccc acagcgactt tttctcgaca ctcgccgaga tgggcgcaat cggcgtgctg
     4141 tgcctgttgc tgttctacta tggcatctcg gtgtacttct ggcgtcacag gctgtcgagc
     4201 gatccggcga ttcgcgcggc gtcctatgct gggttggcgg ttgccaccag cacggtgatt
     4261 ttcggactga ccatcgacgt tctcgtgccg atcatggtca ccgtgctgct cgcgctgctg
     4321 gtcgctacgt ttctcgcggt gatcgattcg cgcaaacgcg aactggctac ggcgcaaccg
     4381 gcagcggcac gcgccgcccg tcaaaccact accggcgtca aagcctgatt cgagcgcggc
     4441 gtggcgtggg gctgacgtcg ctcctacttc ggttcaccgt gggcggctca aacccggcgt
     4501 cgcgctgact tcggcccgat ccaatcccgg cttcggcccg gcttcacacc actttcagcc
     4561 gcgcccggca cctgtgcgcg gcgcggcggc ctcaacgcgt gaacagttcg tagagcgcgg
     4621 cgacatgagc atcgacgccc gggtcgtaga cgaggctccg cagcggctcg gcgatacgcg
     4681 cgtcgccgtc ccgcacacgc tccaccgcgg cctgaatggc gcgctcgaag ctccccgccg
     4741 actgccgcga gaactcgatc ttcgccgcac cggtgacggt ctccgccgat ccgacgttgt
     4801 cggcgatcac aagcggcgtg ccgcacatca ccgactccac acccaccaga ccgaacggct
     4861 cgtatgccga cgccaccacc gtaaagtcgg ctgcggcgaa caatttttcg atctccttgc
     4921 agtacccgac atagcgaatc gtgtcgctgg ttttcggcac gggccggccg gccaccgcaa
     4981 ggcaaaccgg cagcgtggtt tgtgcaaaaa acgcttccag caaaggatag cctttgcgtt
     5041 cgtgactggt cgacgaaaac acgaaaatca cgcggtcctc gggcagaccg aactggcggc
     5101 gcaccgcggc tcgctctgca tcgttcagcg gctgaaagcg cgcggtgtcc accggtgggt
     5161 agagtacgtc gatgcgctcg gccgggacgc cgtaaaaacg ttgcaactcg cggctcatca
     5221 attgcgaatg cgcgacgatc gaacgcgcct gcgaatagac gcgccgttcc atatcgatct
     5281 gccattcgtc gctgcggcgc gcggtgcggc cggcggcttc gagcgaaccc ggatgcgtgc
     5341 cgccgcacaa cgcgacgtcg gcatgcgtgg agtggttgat cgagaaaacg cacgccggac
     5401 ggtgccgttt cagccgctgc ttgagccgcc agtcgaacgc gagattgcga agcttgcgcg
     5461 gcgcccagcg gacattgagc ggctgagcgt cgatccacgc cgcctcaggc agcgtgcggt
     5521 cgatctcgcg ggcgaacaac gtgggacgca ggcccattcg atgcaagccg gcaatcacgt
     5581 cgcgcgtgta gcgttcggcg ccgccactgt ttttcagcgc ctgggcggtc aggacgatgt
     5641 cggtaacagg acgccgggtg ggatcggaca aagatcggtt ctctgtgagg cgcccgcgcg
     5701 gtggaggacc gtgcggactg gttttcagga cagcggcaat tgtaaaggat gggcttcgcg
     5761 ggctacatga cgcagccctc ccgtcaccga ttagaatcta gcgcaaccgc accccaccct
     5821 atttcatgac cgatagcccg tcgaccagcc caccatccgc aacgccggac actgaccgcc
     5881 cgctcgtatc gatcctgctg atcgcctaca accaggaaaa gcagatcgcc gacgcggtgc
     5941 agagcgccct tgcgcaaact tacacgccgc tcgaaatcat catctcggac gacgcctcca
     6001 gcgacgccac gttcgccgcg atcgaggccg cgatcgccgg ttaccgcgga ccacacaaag
     6061 tgattgcgcg ccgcaacgcg tccaaccggg gcatcagcgc gcatctgtcg caaatcgcgc
     6121 aattggctga gggagaactg ctgttcgtag ccgcaggcga cgacatgtcc gcgccgaacc
     6181 gctgtgagcg ggtggtcgac ttctggctcg cccatgatcg ccgccccgat ctgatcgcca
     6241 ccgacctcgc ggacatggac gaaggcggca acgtccacga acggatgtcg ccgaccgaac
     6301 tggacaccta tcgcagtttc gacgactggc tcgccggccg cccgtggctc atcggcgccg
     6361 cgcacacctg gtcgcgccgg ctgttcgagc gcttcggacc catgctgcca ggcgccgccg
     6421 cggaagatca gatcatggtg ttgcgcgcga tcctgtcagg cggggccgcg agtctgcgcg
     6481 agccgctggt gcgctaccgg cgcggcggac tgtctcgcaa acgccgctat ggatcggtgg
     6541 atgagctgat tgcgcgaatg cgccaaagca atcgccatgg actggcggaa ctcgcacagt
     6601 tgcagcgcga cgccgagatc ggcggtgtga gcgaacgcat gcgcgcggcg ctggcgtcca
     6661 agctcgcgcg cgaacaattc attcacgcga tgctcgaagc gggcggtctc gcagaacgta
     6721 tcgggctgct tacgcgcagt cccaacgtga agctcgggct gcgcatccgc atgtttctgt
     6781 atgcgacatg tccggcggtc tacgcgccgg gaatctggct caagcgcatc gtgcggaaaa
     6841 acggccgctg aaaccgctcg ccgcgaggcg cttcaggagg cgctgcgccg cccgcgcaag
     6901 cgacggccga acggctgctg cgcctcgacg ccgaagctca gcagactctt gcgcaagcgc
     6961 cgcatcatct tcattgcccg caggaaggtc tcgccggtgt ggctgaacac cacgaattcg
     7021 agcgcgccgc gccagttttc gatgccgcgc gaaaacgcgt tcatatcgtc gcgcgcgtcg
     7081 agcggcagtt cagccccgaa gcgctgcccg aacaattgaa tctgatccgc gatgccgtcg
     7141 atctgacgcc gcttgctggc gccccgccgc tcggccgccg cgagcttgca ttcgtcgcgc
     7201 gcaccggccg ccgaccgctg attggtcgca ctcgcgccaa ccgcattgct ctcgtgctgg
     7261 cgatatttca gatgcggcgc cgccacgtga cggatctcgc cgaacgatgc caccaccatc
     7321 gccagccacc agtcgtgcat cacgatgccg gcggggaacg gcagcgcgcg ctggagcgcg
     7381 gcgcggttga ccagcatcgc gcagcccggg atatggttgc tcagcagaaa atcccgcaga
     7441 cgctcggcgc ggcgcgactt cgccgtgata tcgaagagcg acttcgacag gcggcacagc
     7501 gtcgcatcca ccaattcgat gtcgcagaat gccagggccg gcgtggcgag gcctcgggcc
     7561 ttttccacgg cttgcagttc gtgcatcagg tcggcgagtt tttccggcgc ccacacgtcg
     7621 tcctgatccg ccagtgcgac gtactcgccg cggcagcccg ccagcgcacg ctcgaaattg
     7681 cgtacgatgc ccacccgttc gccgctcgcg atcaggcgga cctgcacgcg gccggagtac
     7741 gcggccaata attcgggcgt gccgtcggtg gaagcgtcgt cggagaccac cacctcgaga
     7801 ttcggccagc tttgcacttc cagtgaagcc agaagctcgg gcagataagt gcggccgtta
     7861 taggtcgcaa gcgcaatcga aacgagcggc ttgctggtgg gatcgtcgtg atgcatcgct
     7921 agtgggtttt cctctgcgga attcgggcgt cggtcatcag catgcgtttg gtgtgaaaaa
     7981 agcgcaataa agtgagacaa aaacacaaca tcatctcgaa aaatggacga aaaggccggc
     8041 tgacaagcat gtgtattcgg gaagcgaaat gcgatgctac agggaatgcc aagggtgggt
     8101 gccaacggca gccgggcagc gcctgaaagt ggcgctgtcc ggcggtgacg gcatgtgcaa
     8161 aatttggctg atcaggctgc gtcttgctgg aactggatgc ggtgcagatg cgcatacagc
     8221 ccatcttgcg tcagcagctc gcgatggctg ccgcgctcga cgatcctccc cgcttccaac
     8281 accagaatcc ggtcggcgcg ttcgatggtc gacaaacggt gcgcgatcac aagcgtggta
     8341 cggcccttca tcaaggtttc cagtgcggcc tgcacgtggc gttcggattc cgaatccagc
     8401 gccgaagtgg cctcgtcgag aatcaggatc ggcgcatctt tgtagatcgc gcgcgcgatc
     8461 gccagacgct ggcgttgacc gcccgaaagc atcatgccgt tgtcgccgac caacgtatcg
     8521 atgctgttgg gcatcgcctc gacggtgtcc cacaggttag cagcgcgcag cgccgccttg
     8581 accttctccg gatcggccgt ctggccataa gcgacgttgt tggccaccgt gtcgttgaac
     8641 agcacgacgt cctggctcac catcgcgatc tggctgcgca gcgcgtgcag gtcatattcc
     8701 ggcagcgcga cgccgtcgac cagaatctcg ccgccagtcg gatcgaaaaa gcgcggcagc
     8761 aaattgacga gcgtggtctt gccgctgccc gacgggcccg cgagcgcgac catctcaccc
     8821 ggcgccacct tgaacgacac ccggtcaagc gtgtggcggt tatgcgtggc gttgctgcca
     8881 taggtgaacg agacgtcgcg gaattccact tcgccgcggg cgcgatcgag cggcttgccg
     8941 ccgccctccg gttcggacgg ctcgtcgatc aggccgaaaa tcagttcgca cgccgtcatg
     9001 ccgcgctgca gtggctggtt cacgtccatc agatgcttca gcggcgagat gatcagcagc
     9061 atcgacgtga cgaaggcgac aaacccgccg acggtggtct gatccgacga cgactgcacc
     9121 accgcgatgg taatcaccac cgcaagcgcg atcgacgaca ggaactgcgt caacggctgc
     9181 gcaaggccgc cggagaccgt catgcgcatg gcgtagccgc gcagacgctt gctcatcgcc
     9241 gtgaagcggt cggtctcgta ctgttcaccg ttgtgcacct tgacgacctt gtagccaccc
     9301 accgtctctt cgacgatgta cgacagttcg ttggtcagca tttgatgctc gcgattcagg
     9361 cgccgcagac gacggttgat cttgccgacc agccagccga ttcccggcag cagcaccgcg
     9421 acgatcagcg tcaggcgcca gttcagatag aacagataac cgagcaggaa gacgactgtc
     9481 agcgaatcgc gcaccagcgt gaccatcacg ctcaacagca cgttgagaat ctgattcacc
     9541 tcgaagacga tcgcgttgat caccgtgctc gcggtttcgc gctggaagaa cgccacgctc
     9601 gtgtggatca tccggttgaa catcttcagc cgcaattcga gcagaatctt gttcgagacg
     9661 taggcgagca gatagccgga cgcgtattgc gacacgccgc gaatcagcgc aaggccgatg
     9721 accgcaaccg gcacgaacca cttggcgttg tcgctggcat gtgcgccgaa gcccttatcc
     9781 agcagcggct tgagcaacgc gggaatcgct gcgtcggtgg cggagctcac ggccattgcg
     9841 acgactgcgc cgaccaccac ccacatcagc ggcttgatat acggccacaa gcgtcgaaag
     9901 acgacggcgg gcgacgatgc ttcacctgaa ccgatgggtt tgcttaacgt tggcttcgcg
     9961 ctcaaggaat cctctgagaa tgcggaacag catccacgca gcggggcgga tgcccgccgc
    10021 gcggaatatc gaaaaagagc attgtaaatg gttggagacg atgcccgacg gttaaggatg
    10081 gttcggattt gcccggttgc ggcggcgctg aagcgtggtc cgtcggctgc gctgtgtata
    10141 cttgcgcgac ccatttcgca caccgcaccc tcaacgcatt ccagcgcatg caagaaacca
    10201 ctctcggcgt cgccatcatc accaagaacg cggcggcgcg actggccgaa tgcctgcagg
    10261 ccgtggcctt cgccgatcag atcgtcgtga tcgacggcgg cagcaccgac ggtaccgctg
    10321 agatcgcccg ggcacacggc gcgcgcgtgc tgcagcaaac cgactggccg ggcttcggac
    10381 cgcaaaaaaa ccgtgcggtg gacgcgctca ccaccagctg gattctctcg atcgacgccg
    10441 acgaaatcgt ctcgccggaa ctggcctcgg cgatccgcgc ggcgctcacc gcgccgaccg
    10501 ccgatgtcta cgcggtggac aggttgtcga gcttttgcgg ccactggatt catcacagcg
    10561 gctggtatcc cgactggatt ccgcgcctgt tcaaacgtgg cgccgcgcgt ttctccgacg
    10621 acctcgtgca tgaacgcctg gtgttcgaca ccccgtcgca gcgactgacc ggcaagctga
    10681 tgcactactc ctacgaggac ttcgagggcg tcttgcgcaa gctcgacgcc tattcgacgg
    10741 cgggtgcgca gcaacgtcat gcagcaggca agcgtggcag tttcggcaag gcgctcgggc
    10801 gtggcgcatg ggccttcgta cggacctatg tgctgcggcg cggatttctc gacggccgcg
    10861 cgggcttcat gatcgcgttg ttcaacgcgg aaccggtgta ctaccgattt gtgaagctgg
    10921 cgcggctggg ggagcaggcg cggcgccagg actagccggg gctcgtcttc agcgcgggcg
    10981 ccaggcctcg ttcctgagcc agtttcaacg gaacgactgg tcacggccga agcacagtat
    11041 tcgatagcac ggtcaggcgg cagcaacccc agcgtcagtg ccacgaaaat tgatcgactg
    11101 tgcccgcgcg agctatgcaa tcggcgcggg caaaccggtc atcaataaac gatttcgatc
    11161 gagcttccgg caaactcgcc gcgcaatgcc gcgagcagtt cgtcggtcgg cttcacgcgc
    11221 cacgcgtcgc ccagacgcat ttcgccttgc gcattctcgc tgcgataaac cacctgcacg
    11281 gcgaggccgt tcggaatctg caccgcctgc cgctgcccac cgccgccata accaccgccg
    11341 ttgcgcccag cgcctgcgcc acctccattg ccgcgcgatg acgctgccgg cgccggcgca
    11401 gcttgtggct cgtccttacc cgcgctatgc gcttcgagca cgcgccgcaa cgccaatgcg
    11461 tctgcattac cgttcatctg caccttcacg gcttccgcat aacggcaacg cgcgcggccg
    11521 agatccatca ccgtatcgac tgtgaaacgg atgccgcccg taaatgcgtc gttacgcgcc
    11581 tggccttgca ccaccagcaa ttcgtcttcc ttgaacaact gcttgtgcgc ttcaaaggtc
    11641 tcgttgaaga cggtcacttc gcattggccg gtgccgtcgt cgagcagcgc gatcagcatc
    11701 ttgccgcgct gggtcatctg cgtgcgcagc gacgcgatca cgcccgcgac cagcttgtcg
    11761 cgcccttcct tcagttcgcc gattttctga cgcacaaagc ggcgcacttc gtccttgtag
    11821 gcatcgaaca ggtggcccga caggtagaag ccgagcgcgg ccttttcttc ctgcagcttc
    11881 tttttttccg gccattccgg ctcgtcgacc agctcgtgac cttgcgacgg cgcatcgccc
    11941 atgtcgaaca ggcccgcctg catcgcattg gcacttgcct gctcggcggc ttccatggcc
    12001 agcgagacgg aggcgatcag ttgcgcgcga ttcgcgtgca gcgtgtcgaa cgcgccggcg
    12061 cggatcagtg cttccaccgt gcggcgattg acgatccggc gatcgacccg gttgcagaaa
    12121 tcgaagatgt cgatgaacgg accttcttca cgtgcgcgga tgatttcttc gatcgcatt
//
LOCUS       DATASH010000505        18447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2057820,
            whole genome shotgun sequence.
ACCESSION   DATASH010000505 DATASH010000000
VERSION     DATASH010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 18447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18447
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2057820"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(301..1308)
                     /locus_tag="VHV99_13910"
                     /pseudo
     CDS             complement(301..1308)
                     /locus_tag="VHV99_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004556944.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
     gene            complement(1363..1620)
                     /locus_tag="VHV99_13915"
     CDS             complement(1363..1620)
                     /locus_tag="VHV99_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011851594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family NRPS accessory protein"
                     /protein_id="HEX3636557.1"
                     /translation="MTQAQQTPSAAADESDDVVYRVVINDEAQYSIWPTFRAVPAGWR
                     EVGVSGPKAACLAHIDTVWTDMRPASLRRHMDEAASKASTA"
     gene            complement(1751..2422)
                     /locus_tag="VHV99_13920"
     CDS             complement(1751..2422)
                     /locus_tag="VHV99_13920"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-70"
                     /protein_id="HEX3636558.1"
                     /translation="MAEVLIRREPSTAIPMAVVNAGVVNPPGQRRARSHSRTVDLEHD
                     TLLDVLIANRPMLVNLARGFVGCASLAEDVVHDVFIKLVDFPDQDAVRQPLAYVTRMV
                     RNASIDAFRRQSLENTYHADEDDGLHVPSPEPSPEAALLVRDTLRHVYNALEQLPPRS
                     RAAFEMVRLREETLQSTARALDVSQTLVHFMVRDAEKHCADCLDANNRGVAGPVFCSG
                     RARRR"
     gene            2574..2813
                     /locus_tag="VHV99_13925"
                     /pseudo
     CDS             2574..2813
                     /locus_tag="VHV99_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158206.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2821..3129
                     /locus_tag="VHV99_13930"
     CDS             2821..3129
                     /locus_tag="VHV99_13930"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEX3636559.1"
                     /translation="MANAYLQVIAHYFAKPGNGDAIVKPLTELAAATRAEPKNLYYEF
                     FRSPVDPDHFVILEQYSEADGLAVHRDTPHFQRLGFGTIIPLLDRREVTSHMVPGDAA
                     "
     gene            3129..4289
                     /locus_tag="VHV99_13935"
     CDS             3129..4289
                     /locus_tag="VHV99_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014899781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX3636560.1"
                     /translation="MNAFRFQTVPTMVVEFGAARRLGALLRAQFPSLTRLCVVTDGFL
                     HKSGLLNPALADLAAHGWNATVIDDVIADPPEHIVLEASSRARQAGAEIVLGLGGGSS
                     MDVAKLIAVLAPQQQQALGAMYGVNQVEVSRLPLVQMPTTAGTGSEVTAVSIVTVGEA
                     KKMGVVAPQLVADLAILDAELTLGLPVAATAATGVDAMVHAIEAYTSAHLKNPISDML
                     AVKALELLSRNLLPACENGQSREAREAMLLGAAFAGQAFANSPVAAVHALAYPIGGIY
                     HVPHGLSNALVLPHVLRFNADAAAHLYAELADVVVPGLTGSAEAKTLALIERLEQMIA
                     ATAIPARLRDVGIERSGLERMASDAMLQTRLLVNNRRTVTEADALAIYTAAF"
     gene            complement(4286..4483)
                     /locus_tag="VHV99_13940"
     CDS             complement(4286..4483)
                     /locus_tag="VHV99_13940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636561.1"
                     /translation="MLALAYEKPASLATTSFGARTTCVEQTDWDNSCEDSMALSELER
                     RVREGLRAGEFHLVFPAPIAD"
     gene            4914..5192
                     /locus_tag="VHV99_13945"
     CDS             4914..5192
                     /locus_tag="VHV99_13945"
                     /inference="COORDINATES: protein motif:HMM:NF024736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX3636562.1"
                     /translation="MLQIPLTDADWARVQSLFPVAPSGRGRPRRSDREILDAVLWVLQ
                     TKEKWHRLPATFPPQQTCYLRYITWKRTGILDLVNERLPLCGSELSTC"
     gene            complement(5362..6756)
                     /locus_tag="VHV99_13950"
     CDS             complement(5362..6756)
                     /locus_tag="VHV99_13950"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX3636563.1"
                     /translation="MTNSLRMRLLWWLLLPLALYVFVTGKAEYDNARRTADLVQDNQL
                     ISSARMIAGEVEWVDGFLRVDVPPAALEVFVSPYHDQVFYSVSVDDGRLLAGIPDFPQ
                     HAPLSPDKADHYDAELNGQPVRAAGLARLMYDNGATHRVRVTVGKTVRSRDAMAQQLW
                     QPQLVRQIEMIVLAVALVCIGLTFELRPLMKVKEDVVDRDPMQLEPLRVERLHTELRP
                     IVEAINQCIARLGVQVAAQRRFIADAAHQLRTPLTLLGTQLQFARQQDGLNAALDEAL
                     AAMHRSNRSMVGLTNKLLLLAQAEAADNTQLAVETVDLVSLAMEVAEDLALLAQARDI
                     DLGAELNGPALVAGHRGLLQALIANLVENAIRYTGSGGHVTVAVSGAADTVTVSVLDD
                     GPGIPAESRSRVFEPFFRASADTEGTGLGLAIVREIADAHHGEIVLKPGDGGKGVHVS
                     VSFPRAAAQGPRPE"
     gene            complement(6746..7423)
                     /locus_tag="VHV99_13955"
     CDS             complement(6746..7423)
                     /locus_tag="VHV99_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3636564.1"
                     /translation="MKLLLIEDNPTLAHWLAKMLEQEAFALDAVQDGDAADRLLRTNH
                     YDVILLDLNLPKLSGKNVLRRLRQRGDATPVLILTASGSIDEKVELLGAGADDYLVKP
                     FEVRELIARIKVAIRRQSPSKSSEVVCGDLAFDIDTRQFTLKGAPLNVTPRERSVLET
                     LILRLGKTVTKPALVDAIFTLADEPSEDAVEIYISRLRKKLDGSSAAIVTLRGLGYLL
                     RKKEDDQ"
     gene            7720..8739
                     /locus_tag="VHV99_13960"
     CDS             7720..8739
                     /locus_tag="VHV99_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3636565.1"
                     /translation="MRTLRQIAAVSGVAFTLAAASAAAHAEKLTIMVGGATKIIYLPA
                     KLTEQLGYFKDEGLDVDILSQPAGVDAENELLAGAVQGVVGFYDHTIDLQSKGKEIQA
                     LVIFGQVPGEVEMVSTKAADTFKSMADSKGKTLGVTGLGSSTSFLTQYLAQRAGVPST
                     QYTLLPVGADNSFIAAIKQNRIEAGMTTEPTVSQLLKTGDAKVLVDMRTLEGTRAALG
                     GTYPASSFYVQRAWAESHKDDAAKLSHAFARTLNFIATHSAEEIAAKMPKDYYGNNKD
                     LYVGALKASLPMFTKDGKMPADGPDTVLKVLAAFNPSVKGKHIDLAKTYTNDYVSAAV
                     KTAAK"
     gene            8807..9601
                     /locus_tag="VHV99_13965"
     CDS             8807..9601
                     /locus_tag="VHV99_13965"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3636566.1"
                     /translation="MNQPMSRDTPAIELRNVSCRFISPDGKATIALRDFSMSVARGEF
                     VAVVGPTGCGKSTTLSMITGLLKPTTGEVRVMGAPVDGIDPRIGFVFQADAVFPWRSV
                     IDNVAAGPLYRGRSKSAAYDEANEWLRRVGLDKFGKHYPHQLSGGMRKRVALAQTFIN
                     KPEILLMDEPFSALDMQTRTLMQDELLQLWGGAGSVVFVTHDLEEAIALADRVFVLTA
                     RPATLKKVYEIDLPRPRVTSEVRYDPRFIEISRDIWHDLREEVQIG"
     gene            9618..10481
                     /locus_tag="VHV99_13970"
     CDS             9618..10481
                     /locus_tag="VHV99_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3636567.1"
                     /translation="MSTTPQHMLPSGIDATSLAQVERIAQKRIRQRHALVITLRIVVL
                     VVVLGGWELSARLKWIDPFFFSMPSAIFDQIIDWFVNGTSQGPLLTQVWVTLEETGLG
                     FIIGSIGGVFCGIVLGRNKLLSDVFSLYIKIANSIPRVVLGSVFVIALGLGMASKVAL
                     AVVMVFFVVFANAFQGVREADRYMIANAQILGASRRQVTTSVVIPSALSWILASLHVS
                     FGFALVGAVVGEFLGSKQGIGLLISTAQGAFNASGVFAAMIVLAVVALAADYLLTAVE
                     QRLLKWRPAAV"
     gene            10752..11027
                     /locus_tag="VHV99_13975"
     CDS             10752..11027
                     /locus_tag="VHV99_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4148 domain-containing protein"
                     /protein_id="HEX3636568.1"
                     /translation="MKSLIQAIVIAAVLAAPVASFAQSNQPVTRAQVRAELIQLEQAG
                     YRPGLSDPYYPTDLHAAQARVAAQNGTAQGAESGYGGGVSGASQSSH"
     gene            complement(11357..11605)
                     /locus_tag="VHV99_13980"
     CDS             complement(11357..11605)
                     /locus_tag="VHV99_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636569.1"
                     /translation="MRQAFNIAVVLLLGYLMADRALMRAQAGETGTITCHQGAELVKS
                     NALKKGFGDAGASSQGENFLSSCLVTGRGQVGDLIARE"
     gene            complement(11630..12376)
                     /locus_tag="VHV99_13985"
     CDS             complement(11630..12376)
                     /locus_tag="VHV99_13985"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEX3636570.1"
                     /translation="MAPTGKQHCDTLDLIRALAAGAVCVSHLRNLMFVDYRASVGIGL
                     AGKAFYFVSNYGHTAVIVFFLLSGYFVGGSVLRQVEVGTWSWQRYLTERMSRLWMLIP
                     ALLLTLFWDRMGIALAGGPFYLGTEGTFDQQINVASHLGQSTLLCNLVFLQTLTCGTY
                     GSNGPPRRAGVRDHAGCLLWVRVRGVPAVRTEYETCATWYPAVPFPAGQAGSSHAAEL
                     ICVVLPLDLICIIECPSIGKPLASCIDGSR"
     gene            complement(12578..13624)
                     /locus_tag="VHV99_13990"
     CDS             complement(12578..13624)
                     /locus_tag="VHV99_13990"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HEX3636571.1"
                     /translation="MPNPTRAFLLGPLLKGVSRSFYLTLRVLPAGMRDPIGLAYLLAR
                     AADTIADTSLISPEQRLALLLSLRDQVNGAPDDGALFRRMAAEVAAQQVQSDEKVLLE
                     SLGPALEVLAQLSESDRKAVREIVSTLTEGMEFDLRTFPDERSGQIAALREYDELDRY
                     TYLVAGCVGEFWTSMTYAHMPGTLKERPETMARRGVRFGKALQMTNVLRDCSKDLRIG
                     RCYLPQTMLDPYALTAQDLLQPANSLRARPLMIELVRKTLAHFREALDYTLAIPAFSV
                     RLRLACLWPILIGLETLLLLVDNDAWLDPAKVSKVRRNQVYRIIASSLLLVASNGLVR
                     KSVETRIEQIEARL"
     gene            14148..15623
                     /locus_tag="VHV99_13995"
     CDS             14148..15623
                     /locus_tag="VHV99_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEX3636572.1"
                     /translation="MTIRDSQTSSRSNVETGVPGLDEILGGGLVNGGVYLLEGMAGAG
                     KTILSSQIGFHRVSQGEKVLYMTLIAESHDKLLAHLRGLSFFDEAAVAQQMLFVSGYH
                     ELMQGGLDGFLKLIASSIYDYRPSLMIIDGFRSAREFSETELSLSKFIHELNALAAAM
                     NCTTLLLAPLSGNEQHPEHTLVDGLIELNRHGDGMRRAREIEVHKMRARNHLMGKHFF
                     RIAESGLLMFPRLESLDAVQPGPVDLKTRLGFGLPHLDNLLGGGFTQGSTTTLIGQSG
                     VGKTLLCLQFLAAGLVRGEPCLYLGFYEGPQRLIGKAEAVSIELADAYHDGRLMIQWQ
                     PAIELAVDELATIALATVKQTGVTRIVIDGVEGFRDSALRPERFGLLLNALLHQLREA
                     CVTTILTEELPLYAGPGHAKSVRVSALTENLVLLRYAETETGLRRMISVVKQRESAHD
                     TSLRELVISSQGLDVIDDPADTPSVYPAPGLAAALTPRRDT"
     gene            15630..15980
                     /locus_tag="VHV99_14000"
     CDS             15630..15980
                     /locus_tag="VHV99_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3636573.1"
                     /translation="MKTILVVDDEFDILTVWRLLLERHGYTVLTASNGAAALEQIRKT
                     RPDIIVSDCMMPIMSGLQLCAALNDDPDLRAIPIILCSAAADIPAQPNPTIEYARKPL
                     SFDSLLAMLERMTV"
     gene            16106..17539
                     /locus_tag="VHV99_14005"
     CDS             16106..17539
                     /locus_tag="VHV99_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3636574.1"
                     /translation="MASLPLDAPGASPDSSAGAQGLTTGINARIDRLPATRTVWMLVF
                     LLSIGGWFEFYDLFFTAYVGPGLVKSGLYATTTASFFGVSGLGAFVAASFAGLFIGTF
                     FFAGMADRYGRKTVFTVSLLWYSVATLIMALQTTAPVINLWRLIAGIGVGVELVTIDT
                     YVSELVPKHLRGRAFAFVHLVQYTAVPSVAFLAWWLVPQSPFGLDGWRWVVIIGALGA
                     VVVWAIRRRVPESPRWLAQQGRAAEAEKVLQALEAKIASQYGKPLPPAVPTVEPATTK
                     AAFREIWQPPYRKRAITMLVFNLFQSIGFYGFASWVPTLLVSKGVTITHSLLYSFVIA
                     ISNPFGPLIGMAIADRIERKTLIVLSALGIAVFGSLFATQTSPAMLMTLGVLITLCGT
                     LLSVGYHAYQTELFPTRLRARAVGFVYSMSRLSAMFSGFMIAFALRHFQVPGVFALIT
                     VSMVMVMGAIGIFGPRTNNRNLDEISH"
     gene            17556..>18447
                     /locus_tag="VHV99_14010"
     CDS             17556..>18447
                     /locus_tag="VHV99_14010"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX3636575.1"
                     /translation="MTLPLAGVRVLDLSNVLAGPFCAYQLALLGAEVIKVEHPEGGDL
                     ARRLGADKEASMRNMGASFVAVNAGKQSITLNLKDPRGKTILRELVKTADVLVENYRP
                     GVMTRLGLDFDALREVNPKLVYCAISGFGMDGEFSKRPAYDQIIQGISGVMSVTGDAD
                     SAPLRVGYPVSDTVGGLTAAFGICAALVDARASGHGRMLDVSMLEATLATMGWVVSNY
                     LNAGVTPEPMGNENFTAAPSGTFKTGDGPLNIAANENKQFASLCHLIGRADLLDDARF
                     SERNARKVNRAALKAEIEAAL"
BASE COUNT         3489 a         5754 c         5653 g         3551 t
ORIGIN      
        1 gccgcctctg cggcgcgccg cgtggccgcc tcggcctcca ggcgggcgga ttcttccgcc
       61 gcgagccggg cctgttcctc ggcgtcgcgt cgtgcaaggg cctcggcttg ccgacgggcg
      121 agcgcctcga cgtcgcgacg ggcctgtgcc cgtgcttccg cttcagcttc tgcttgcgcc
      181 tgtgcctgtg cctgtgcctg cgcctgtgcc tgtgcctgtg cctgtgcctg tgcctgtgcc
      241 tgtgcctgtg cctgcgcctg cgcctgcgcc tgcgcctgcg cctgcgcctg cgcctgcgcc
      301 tgcgccttta ttgtgacctt cgctatagct ctgcgccatc gcgacgacaa ccttgcgcgg
      361 cccttcgaac ggatcgcgcg cgtgcgccgt cagcatgttg tcgagcatca gcacatcccc
      421 cgtcaaccat ggaaacacaa tgcgctgctg atcgagcaca ccgcggattt ccgccagcgc
      481 ctccgcttcg agcggctcgc cgtcaccgta atagacgttg cgcggcacgt tttcaagtcc
      541 gaccgaatcg attaacgctc cctgcatgtc gtcatcgagc gaagagaggt ggaacagatt
      601 cgcctgattg aaccagacca tctcgccggt gcgcggatgg cgcgcgaccg cttgacaacg
      661 ttcgcgcgta cgcaacagca gttcgccatc ctcactgtcg cgccattcgc aatcgatgcc
      721 acgtgtgcgg caaatccgct cgaccacgct cgggtcgtcc gtaccgaacg cttgttccca
      781 cggcagatcg agcccttgcc caaagttgcg tatgtacagc agctcacgct tcgcaaaacg
      841 tgcgacgagc gccgggtcga gcgcccgata aatcgcgcgg ctgtcggcaa tcggtgtcgc
      901 gccgcccgag cgcgcggcaa gcgcgcagtg gaaccagatg cgcagcggcc attcgcgcgt
      961 gtatgactgc tcgttgtgca agggaatcga gcgatgcggc ggatattccg tcgatgtgta
     1021 caccgcgcct gccacctgac tgcgcggcgt ggaagcgaat tcgtagccga tcaacggatg
     1081 cccgaacgaa ccggcaaagt tttggaaggc ttcgatcgag gccacgcgaa agccggtgaa
     1141 cagcacgccg ccggcccgct cgagcgtgtc gtcggcgatc gcatgcaaca gcgtcacggc
     1201 ctcttccaat gaaatctccg cgcccgagcg cggcgcgacg acagtcggca agcccggctc
     1261 gatcgacagg tcggcgagcg tgggtagcgg catcgaaagc atggtcatgc gaaatcctct
     1321 tgcagaagcg acgtggtccg gggcttgtcc ggatcggtcc gatcaggctg tgctcgcctt
     1381 cgaggcggcc tcgtccatgt gacggcgcaa gctggccgga cgcatatcgg tccataccgt
     1441 gtcgatatgc gcgaggcagg cggccttcgg tccactcacg cccacttcgc gccaacccgc
     1501 cggcacggcg cggaacgtcg gccagatcga gtactgcgct tcgtcattga taacgaccct
     1561 gtagacgaca tcgtccgact cgtctgctgc ggcggaaggg gtttgctgtg cttgcgtcat
     1621 gatgaatggc ccagtcgatg aatgatcagg atgcctcagc agattgccga tggactactc
     1681 caaagctgcg gtggcagcat cgcgctcgcc caccgcctct actcaattga cgtttgatgc
     1741 gcgcgctttt ttaccggcgc cgggcgcggc cgctgcagaa gaccgggccg gccacgccgc
     1801 gattgttggc gtcgaggcag tcggcgcaat gcttctcggc atcgcgcacc atgaaatgca
     1861 caagggtttg cgaaacgtcg agcgcgcgcg ccgtgctttg caacgtttcc tcgcgcaagc
     1921 gcaccatctc gaacgccgcg cggctgcgcg gcggcagttg ctcgagcgcg ttgtagacat
     1981 ggcgcagcgt gtcccgcacg agcagcgccg cttcaggcga aggctccggc gaaggcacgt
     2041 gcaggccgtc gtcttcgtca gcgtgataag tgttttcgag gctttggcgg cggaatgcat
     2101 cgatcgacgc attgcgcacc atgcgggtta cgtaggccag cggttgccgc acagcatcct
     2161 gatccgggaa gtcgaccagt ttgatgaaga cgtcgtgcac cacgtcttcc gcgagactcg
     2221 cacaacccac aaagccgcgc gccagattga caagcatggg ccggttggcg atcagcacgt
     2281 caagcagcgt gtcgtgttcc agatcgacag tgcgggagtg gctgcgcgcc cgccgctgcc
     2341 ccggaggatt gacgacgccg gcgttgacaa cagccatcgg tatcgctgtt gaaggctccc
     2401 gccggatgag cacctcagcc atggattcgc tccgtatttc tgaaggaagg ctgtcaatct
     2461 atcgtaaacg cgaatcattc ccaatcaaga ataggaataa ccttacattt ctgatagttt
     2521 tgtctgatga aatagggata cctacggcct ggtgttgagg atggatactc aagccgaatc
     2581 tggtacaact gatgcctgcg ccgatgctct cgcatcggtc cgcgcaagac cgcaaagctt
     2641 tgccgatcgt cacctcgggt ttcgtcttca ataagggcgg cgggttcgat gcgttgaaga
     2701 acggcgcgct cgaagatcga ccagcgacag cgacagcgac agcgacagcg acagcgacag
     2761 cgaattgtgg aatctggacc ttgaacgttg aaggtcccgt cttgttggag taattgattg
     2821 atggccaatg cttatctgca ggtgatcgcg cattatttcg ccaagcctgg caacggcgac
     2881 gcgattgtga agcccctcac ggaactcgcg gcggcaaccc gcgcggagcc caaaaatctc
     2941 tactacgaat tcttccgttc gccggtcgat cccgatcact tcgtgattct cgaacagtac
     3001 agcgaggccg atggccttgc cgtgcatcgc gatacgccgc acttccagcg gctcggcttc
     3061 ggcaccatca ttccgctgct cgatcggcgt gaagtcacga gccacatggt gcctggagac
     3121 gcggcatgat gaacgcattc cggtttcaaa ccgtcccgac catggtcgtc gagttcggcg
     3181 cggcgcgccg cctcggcgct ttgttgcgcg cgcaatttcc gtcgctcacg cgtttgtgcg
     3241 tggtgacgga tggtttcctt cacaagagcg ggttgctgaa tccggcgctc gccgatctgg
     3301 cggcacatgg ctggaacgcc accgtgatcg acgacgtgat cgccgatccg cccgagcaca
     3361 tcgtgctcga ggcaagctcg cgcgcgcgtc aggctggcgc ggagatcgtg cttggacttg
     3421 gcggcggttc gtcgatggat gtggcgaagc tgatcgccgt gctggctccg caacaacagc
     3481 aggcgctcgg cgcgatgtac ggtgtcaatc aggtcgaggt gtcgcgcttg ccgcttgtgc
     3541 aaatgccgac gaccgcgggc accggctccg aagtcacggc cgtatcgatc gtgacagtgg
     3601 gcgaagcgaa gaagatgggc gtggtcgcgc cgcaactggt cgccgatctg gcgattctcg
     3661 acgcggaact cacgctcggg ctgcccgttg cggcaaccgc ggcaaccggc gtcgacgcaa
     3721 tggttcacgc gatcgaggcg tacacctccg cgcatttgaa gaatccgatt tccgacatgc
     3781 tggcggtgaa ggcgcttgag ctcctgtcgc gcaatctgtt gcctgcgtgc gagaacggcc
     3841 agagccgcga ggcgcgcgaa gcgatgctgc tcggcgcggc gttcgcgggc caggcgttcg
     3901 ccaattcgcc agtggccgcg gtgcatgcgc tcgcgtatcc gatcggcggc atttatcacg
     3961 tgccgcacgg tctttcgaat gcgcttgtgt tgccgcatgt gttgcgcttc aacgccgacg
     4021 cggcagctca tttatatgcc gaactcgcgg atgtggtcgt gccgggcctg acaggtagcg
     4081 ccgaagccaa gacgctcgcg ttgatcgaac ggctcgaaca gatgattgcc gcgaccgcga
     4141 ttccggcgcg gttgcgcgat gtcggcatcg aacgtagcgg actcgagcgg atggcgagcg
     4201 acgcgatgtt gcaaacgcgg cttctggtga ataaccggcg cactgttacc gaggccgacg
     4261 cgttggcgat ctataccgct gcgttttaat cggcgattgg cgcaggaaac acaagatgaa
     4321 actcgcccgc gcgcagacct tcacggaccc ggcgttcaag ttcgctcagt gccatggaat
     4381 cttcgcacga gttgtcccaa tcggtctgtt caacgcaagt ggtccgtgcg ccgaacgaag
     4441 tagttgcgag cgatgcaggc ttttcatagg cgagcgcgag catggtggaa acgcaaattt
     4501 ttaacctcgc aaacgtatgc acatctcgtg ccagaaaatg cgttcatttt gcaactaaat
     4561 ggcgacacgc gtcgataagc cttattggtc aacgactttg cggcaatacg atcaaacctg
     4621 ttgatacgta atgtcgatcg gcaaacgttt gaacgaaggc gaatgctata gcgttctgca
     4681 tcggcgctac ggctgcattg cgcagtgagt tgaaagtcgc cgttatgaca cgttttcacg
     4741 tgccaagaaa cagtgtgaaa catcacgaat aagattgcca tgcaacaagc ggtgtaataa
     4801 ggtatcgcgc tgcaacaaat taatttgacc ggaaaaaaag atttactagt atcgccaaga
     4861 acgcagcatg caatgacatg actgcgatcc cacacagtca cacaaggcag cagatgctcc
     4921 agattcctct cactgacgcc gattgggcgc gcgtccagag tctttttcca gtggctccat
     4981 ccgggcgcgg gcgacctcgt cgcagcgatc gtgagattct cgacgcagta ctttgggtgc
     5041 tgcaaacaaa agaaaaatgg cacagattgc cggccacctt tccgccgcaa cagacttgtt
     5101 atcttcgata cataacctgg aagcgaacgg gcatactaga cctggtgaat gagcgcctgc
     5161 ccctgtgcgg ctcggagctt tcgacctgct gatcaagcgg caagcattgc ttcgcatccc
     5221 gtatcgattc agaagcgaac cgctggttcg cggatggtct tcgggcggcc tcttctctat
     5281 tgcgcgcgct tggcaaccat gagattcgcc gtgctttgaa gcggaagcgc tgatttgaaa
     5341 cggcgaattc aatcggctgg tttattcagg ccgaggtcct tgcgctgccg cacgcggaaa
     5401 tgaaacgctc acgtgcacgc ctttgccgcc gtcgcccggc ttgagcacga tctctccgtg
     5461 atgcgcgtcc gcgatttcgc gcacgatggc gaggcccaag cccgttccct ccgtgtccgc
     5521 cgacgcccgg aaaaacggtt cgaacacgcg gctgcgcgac tcggccggaa tgccggggcc
     5581 gtcgtcaagc accgatacgg tgacggtgtc agccgccccg ctgaccgcca cggtgacgtg
     5641 gccaccgctg ccggtatagc ggattgcgtt ctccacgagg ttggcgatca gcgcctgaag
     5701 caggccgcga tgcccggcta ccagcgcggg accattcagc tccgcgccca gatcgatgtc
     5761 ccgggcctgc gccagcagcg ccagatcttc cgccacttcc atcgccagcg acaccagatc
     5821 gacagtttcc accgcgagtt gtgtgttatc ggccgcttcg gcttgcgcga gcaagagcag
     5881 cttattggtg agtccgacca tcgagcgatt actgcggtgc atggcggcga gcgcttcgtc
     5941 gagcgcggcg ttcaggccgt cctgctgacg tgcgaactgc aactgcgtgc cgagcaacgt
     6001 gagcggtgta cgcaactggt gcgcggcgtc ggcgataaaa cgccgctgtg ccgccacctg
     6061 cacgccgagc cgcgcgatac attgattgat cgcttcgacg atgggccgca actcggtatg
     6121 caggcgctcg acgcgaagcg gctcgagttg catcggatcg cggtcgacga cgtcttcctt
     6181 caccttcatc agcggccgca gttcgaaggt gaggccgatg cagaccagtg cgaccgccag
     6241 cacgatcatc tcgatctgcc gcacgagttg cggctgccac aactgctgcg ccatcgcatc
     6301 gcgcgagcgc acggtcttgc ctactgtgac tcgcacgcgg tgcgtcgcgc cgttgtcgta
     6361 catcaggcgt gccaggccgg ccgcgcgcac aggctggccg ttaagttcgg cgtcgtagtg
     6421 atcggccttg tcgggcgaga gcggcgcgtg ctgcggaaaa tccggtatgc ccgccagcag
     6481 ccggccgtcg tcgacactca cgctgtagaa cacctggtcg tgatacggcg acacgaacac
     6541 ttctagcgcg gcgggcggca catccacacg caaaaagccg tctacccatt ccacttcgcc
     6601 agcgatcatc cgcgccgacg aaatgagctg attgtcctgt acgagatccg ccgtgcgccg
     6661 cgcgttgtcg tactccgcct tgccggtgac gaacacgtac aacgcgagtg gcagcagcag
     6721 ccaccacagc agacgcatgc gcaggctatt ggtcatcttc tttcttgcgc agcaggtaac
     6781 ccagcccacg caacgtgacg attgcagccg aactgccgtc gagtttcttg cgcagccgtg
     6841 aaatgtagat ttcgaccgcg tcctcgctcg gctcgtcggc gagcgtgaag atcgcatcga
     6901 ccagtgccgg cttcgtcacc gtcttgccca gacgcaggat cagcgtttcg agcaccgagc
     6961 gctcgcgcgg cgtgacgttc agcggcgcgc ccttgagcgt gaactgacgc gtgtcgatgt
     7021 cgaacgcgag atcgccgcac acgacctcgc tcgacttcga cggcgactga cgccggatcg
     7081 ccaccttgat ccgcgcaatc agttcgcgca cttcgaaagg cttgacgagg taatcgtccg
     7141 cgccggcgcc gagcagctcc actttttcat cgatcgaacc gctcgccgtc aggatcagca
     7201 ccggcgtagc gtcaccgcgc tggcgcaaac ggcgcagcac attcttgccc gacagcttcg
     7261 gcagattcag gtcgagcagg atcacatcgt agtgattggt gcgcagcaag cggtcggcgg
     7321 cgtcgccgtc ctgcacggcg tcgagcgcga acgcctcctg ctccagcatc ttcgcgagcc
     7381 agtgcgcaag cgtggggttg tcttcgatga gcagcagctt catggcaggg gacggcgaat
     7441 tggaaggcga acaggcggcg aatgagcagc aaaagtcagg acgccgattg tgccgtaaaa
     7501 caagggcttt gcgcgcggcc tgggcggttt gcatcgcacg tcatggggtt ttccagaggg
     7561 gcgccacgag gttgtggtga attctcgggt taacacccat gatttgccca ctgccccgaa
     7621 agctgccaga aggtttgcga tttttataat ccacgtcatt catccagaaa gcgccgcatt
     7681 ttcgcacccc gcggcgaaca accctaagga gactgcttca tgcgtacctt gcgccagatt
     7741 gctgccgtgt caggtgttgc gttcaccctt gccgccgcct cggccgcggc ccacgccgaa
     7801 aagttgacga tcatggtcgg cggcgccacc aagatcatct atctgccggc caagctcacg
     7861 gaacagctcg gctacttcaa ggacgaaggc ctcgacgtcg acatcctctc gcaaccggcc
     7921 ggcgtggatg cggaaaacga gttgctcgcg ggcgcggtgc aaggcgtggt gggcttctac
     7981 gatcacacca ttgacctcca aagcaagggc aaggaaatcc aggcgctggt gattttcggc
     8041 caggtgccgg gtgaagtgga aatggtctcc accaaggccg cggacacttt caagagcatg
     8101 gccgactcga agggcaagac gctcggcgtg accggcctcg gctcgtcgac cagcttcctc
     8161 acgcaatacc tcgcgcaacg cgcgggcgtg ccgtcgacgc aatacacgct gctgccggtg
     8221 ggcgcggaca acagctttat cgccgccatc aagcagaacc gtatcgaggc cggcatgacc
     8281 accgagccga ccgtctcgca gctgctgaag accggcgacg ccaaggtgct ggtcgacatg
     8341 cgcacgctgg aaggcacgcg tgccgcgctc ggcggcacct acccggcttc gagcttctac
     8401 gtgcagcgcg cctgggccga gtcgcacaag gacgacgcgg cgaagctctc gcacgccttc
     8461 gcgaggacgc tgaacttcat cgccacgcac agcgcggaag agatcgccgc gaagatgccg
     8521 aaggactact acggcaacaa caaggacctg tacgtcggcg cgctgaaggc gtcgctgccg
     8581 atgttcacga aggacggcaa aatgcccgcg gacggcccgg acaccgtcct gaaggtgctg
     8641 gcggccttca atccttcggt gaagggtaag catatcgacc tcgccaagac ctacaccaac
     8701 gactacgtgt cggcggcggt caagaccgcg gcgaagtaac gccctcgaag tcacgccctc
     8761 gaagtaacgc cctatgacca tccagagaac cgcgaggccc cagccgatga atcaacctat
     8821 gtcacgcgat acgcccgcca tcgagctgcg caacgtatcg tgccgtttca tttctccgga
     8881 tggcaaggcg acgatcgcgt tgcgcgactt cagcatgtcg gtggcgcgcg gcgagttcgt
     8941 cgcggttgtc gggccgacgg gttgcggcaa gtcgaccacg ctcagcatga tcaccggttt
     9001 gctgaaaccg accaccggcg aagtgcgcgt gatgggcgcg ccggtggacg gcatcgatcc
     9061 gcgtatcggc tttgtgtttc aggccgacgc cgtgtttccg tggcgctcgg tgatcgacaa
     9121 cgtagcggcc ggcccgctgt atcgcggccg ttcgaaatcc gccgcctacg acgaggccaa
     9181 cgaatggttg cgccgtgtcg gtctcgacaa gttcggcaag cactatccgc atcaactgtc
     9241 gggcggcatg agaaagcgcg tggcgctggc gcaaaccttc atcaacaagc cggaaatcct
     9301 gctgatggac gagcccttct cggcgctcga catgcaaacc cgcacgctga tgcaggatga
     9361 actgctgcaa ctgtggggcg gggcgggctc ggtggtgttc gtcacgcacg atctcgaaga
     9421 agcgattgcg ctcgcggatc gcgtgttcgt gctgaccgcc cgcccggcga cgctgaagaa
     9481 agtgtacgaa atcgatctgc cgcgtccgcg cgtcacgtcg gaggttcgct acgacccacg
     9541 ttttatcgaa atctcgcgcg acatctggca tgacctgcgc gaagaagtac agatcggtta
     9601 atcaaggaac ggcaaacatg tctacaaccc ctcaacacat gctgccttcg ggtatcgatg
     9661 cgacgtcgct cgcacaagtc gagcgcatcg cgcaaaaacg catccggcaa cgccatgcgc
     9721 tcgtgatcac gctgcggatt gtcgtgctgg tggtggtgct cggcggctgg gagttgtccg
     9781 cgcgcctgaa gtggatcgac ccattcttct tctcgatgcc gagtgcgatc ttcgatcaga
     9841 tcatcgactg gttcgtgaac ggcacgtcgc aaggaccgct cctgacacag gtgtgggtga
     9901 cgctcgagga aaccggcctc ggcttcatta tcggttcgat cgggggcgtg ttctgcggca
     9961 tcgtgctcgg ccgcaacaag ctgctctccg acgtgttcag cctctacatc aagatcgcca
    10021 actcgattcc acgcgtggtg ctcggctcgg tgttcgtgat tgcgctcggt cttggcatgg
    10081 cttcgaaagt ggcgctggcg gtggtgatgg tgttcttcgt ggtgttcgcc aatgcgtttc
    10141 agggcgtgcg ggaggcggac cgctacatga tcgcgaatgc gcagattctg ggtgcgtcgc
    10201 gccgtcaggt gacgacctca gtggtgattc cgtcggcgtt gagctggatt cttgccagct
    10261 tgcacgtgag cttcggcttt gcgctggtcg gtgcggtggt gggcgagttc ctcggctcga
    10321 agcaaggtat cggtctgctg atctccaccg cgcaaggcgc gttcaatgca agcggcgtgt
    10381 tcgcggcgat gattgtgttg gcggtggtcg cgctggccgc ggactatctg ttgaccgcgg
    10441 ttgaacagcg attgttgaag tggcggcctg cggcggtttg agtcgaccgt cagatcgtgt
    10501 agagagaagg ggcgccagtg gcgccctttt ttcatttcgc gctgtcccat tcaggatggg
    10561 tggtcgatac ttgaattgct ccgcagcaag atactcccct ctctcaagcc tcttcatttg
    10621 tctcaaattt agcaataaac cttgcctgaa tgagtgatta ctcccgccag atcgcaaaac
    10681 tacactgtaa tcaccgactg cgaacaagat cgctcgtagc acgataaaga ccgaactgga
    10741 ggtagttcat catgaaatcg ctcatacaag ccattgttat cgccgctgtg ctggctgctc
    10801 cggttgcctc gtttgcgcag tccaaccagc ccgtgacccg cgcccaagtg cgcgcagaac
    10861 tgattcaact cgaacaggcc ggttaccggc cgggcctttc cgacccgtac tacccgactg
    10921 acctccatgc cgcccaagcc cgggtggccg cgcaaaacgg caccgcgcaa ggcgcagaaa
    10981 gcggctacgg cggtggggtg agtggcgcgt cgcagtcgag ccactgagca ggcaaccagg
    11041 cgggtagccg agttcggtta accacgccgg caaccaggct ggtggcgaag ctgacccagc
    11101 ctgtccagcg gaacgaaccg cagaggccac gcggattgcc ctgatctgat ccgccgctaa
    11161 gcgcgcaaac agccgcgcgc tctgattcaa acgaactggc ccgcgatcga gtgtccccga
    11221 tcgcgggcca gttctgtatt cgatatctct gtcatcgggc tcccgatgac ttcgccggga
    11281 ccctcgcggt ctgggcactt ttcgcgtatg taccgccgcg cttcgtcgaa gcctcagcga
    11341 tgccgggctg cttgcatcat tcgcgggcga tcaggtcgcc cacctggcca cgccccgtga
    11401 cgaggcagct cgacaggaaa ttttcaccct gactgctggc gccggcgtcg ccgaaacctt
    11461 ttttcaacgc attcgactta accagctccg cgccttggtg gcacgttatt gtccccgttt
    11521 ctcccgcttg ggcgcgcatc agcgcccgat ccgccatcag ataaccaagc aacaacacca
    11581 cggctatatt gaacgcttgt ctcatggatc gtctcctctt ttgaagagac taacgcgaac
    11641 catcgatgca ggatgctagg ggtttcccga ttgatggaca ttcaatgatg cagattaaat
    11701 cgagtggcaa tactacgcag atcagctctg cggcgtggct ggatccggct tggcccgcag
    11761 gaaacggaac tgccggatac cacgttgcac acgtttcgta ttctgttcga acagcaggta
    11821 caccgcgaac gcgtacccat agcagacaac cagcatggtc acgaacacca gcgcgccgcg
    11881 gcggtccatt tgagccgtac gtgccgcacg tgagcgtctg caagaacacg agattgcaca
    11941 gcagtgtcga ttggccgaga tgcgaggcga cgttgatctg ctgatcgaat gtgccttcgg
    12001 tgccgagata aaaaggcccg cctgccagcg cgatgcccat gcggtcccag aacagcgtca
    12061 gcagcaaggc cggaatcagc atccatagcc gcgacatgcg ctcggtcaga tagcgctgcc
    12121 agctccatgt ccccacctcg acctgccgca gcaccgagcc accgacgaaa taaccgctca
    12181 gcaggaagaa cacgatcacc gcagtgtggc cgtagttgct gacgaaatag aaggccttgc
    12241 ccgcgagtcc gatgccgacg ctcgcgcggt agtcgacaaa catcagattg cgcaaatgac
    12301 tgacgcacac cgcgcctgcc gccagcgcgc ggatcaggtc caacgtgtcg caatgttgtt
    12361 tgccggtggg tgccatggaa gtctggaatc ggacaaggtg gtttgtccgc caaactacgg
    12421 ccggtggggg ttttattcag tacgcttgcc cacactgcac gcgcacggca catacgcggc
    12481 acatgcgtcg cacgcaaaca cgcgattcat tgaggtattg ctggggaccc ggtccgcgcg
    12541 caacgttgcg aactggttcg tatagcgaag cagccgctca gagtcgagct tcgatctgct
    12601 cgatccgcgt ttcaaccgac ttgcgcacca ggccattcga ggccaccagc agcagtgacg
    12661 atgcaatgat ccgatacacc tgattgcgcc ggactttaga gaccttggcc gggtcgagcc
    12721 aggcgtcgtt atccaccagc aacagcagcg tttcgagacc aatcagaatc ggccacaaac
    12781 aggcaaggcg caagcgcacc gaaaacgcgg gaatcgccag cgtgtagtcc agggcctcgc
    12841 ggaaatgagc cagcgtcttg cgcacgagtt cgatcatcaa aggccgggcg cgaagagaat
    12901 tcgccggctg aaggagatcc tgcgcagtga gcgcgtatgg atcgagcatc gtctgcggaa
    12961 gataacagcg gccgatccgc aagtccttgc tgcaatcgcg cagcacgttg gtcatctgca
    13021 gcgccttgcc gaaacgcacg ccgcggcgcg ccatcgtctc gggccgctct ttcagcgtgc
    13081 cgggcatgtg cgcgtaggtc atgctggtcc agaattcgcc gacgcagccc gcgacgagat
    13141 aggtatagcg gtcgagttcg tcgtattccc gcagcgcggc aatctggccg gaacgttcgt
    13201 cggggaacgt gcgaaggtcg aattccatgc cctcggtcag ggtcgacaca atctcgcgta
    13261 ccgccttgcg atcagactcg ctcagttgcg caagcacctc tagcgcggga ccaagcgatt
    13321 ccagcaagac cttttcatcc gactggacct gttgggccgc cacttcggcc gccatgcgcc
    13381 ggaacagcgc gccgtcgtcc ggcgcgccgt tgacctggtc gcgaagcgac aacagcaacg
    13441 caaggcgctg ctcgggcgag atcagcgagg tatccgcgat cgtgtcggcc gcgcgcgcca
    13501 gcaggtatgc gaggccgatc ggatcgcgca tgccggcggg cagcacgcgc agggtgagat
    13561 agaaggagcg tgaaacgcct ttcaggagcg ggccgagaag aaaggcccgg gtcggattcg
    13621 gcatggatgg gatcaggtca ggattgggcg gaaatcaggc gctgccgaat tgtatacggc
    13681 gaagcgcctt tgtcgtgcag cggcggtcct gtcgtggctg cgcgctgacc cggcggttca
    13741 cacctgcgaa cccgttgctg gtgaaccctc tcgccccgag ttacccgcgg cacgccatcg
    13801 cctgcccgac tacgctatct gcagtttgcc cgcgcggtct cttcgatctg gctgacgttc
    13861 cgcgggtccg caattttcgt tgcaccgttt cgcatcgctt tgaaaaaccc gcttccgcac
    13921 tccgccgata cttgccggta agcagcaccc ctcgcgcacg cattgaaccg cctgcccatg
    13981 cctcgtcccg cacgtgggca gaggccaggt aaggccccct gcacttactg ccgacacgcg
    14041 tcctgcagcc cgcctgcggg aatgccaatt gctgacacgg ttagtttgag cgatgcgcag
    14101 tctcgttgcg cgctgtcgct cctccgcact ttgagcgaca tacgacaatg actatccgcg
    14161 actctcagac atcctcccgg tccaacgtgg aaacaggtgt ccccggtctc gacgaaattc
    14221 tgggtggagg tctggtcaac ggcggcgtct atctgctgga aggcatggcc ggcgcgggca
    14281 aaacgattct gtcgagccag atcggttttc atcgtgtgag ccagggcgaa aaggtgctgt
    14341 acatgacctt gatcgccgag tcgcacgaca aactgctggc gcacctcaga ggcctgagct
    14401 tcttcgacga agccgccgtc gcccagcaaa tgctgttcgt ctccggctat cacgaactga
    14461 tgcagggcgg tctcgatggc ttcctcaagc tgattgcgtc gagcatttac gactatcgcc
    14521 cgagcctcat gatcatcgac ggctttcgca gtgcgcgcga attcagcgaa accgagttgt
    14581 cgctgtcgaa gttcatccac gaactgaatg cgctggccgc ggccatgaat tgcaccacgc
    14641 tgttgctcgc gccgctctcg ggcaacgaac agcatccgga gcacacgcta gtcgacgggc
    14701 tgatcgagct gaaccgccat ggcgacggca tgcgccgcgc acgcgaaatc gaagtgcaca
    14761 agatgcgggc gcgcaaccat ctgatgggca agcatttctt tcgcatcgcc gaaagcgggc
    14821 tgttgatgtt cccgcgtctg gaatcgctgg acgccgtgca accgggtccg gtcgatctga
    14881 aaacgcggct cggcttcggc ctcccgcatc tcgacaacct gcttggcggc ggtttcaccc
    14941 aaggctccac gaccacgctg atcgggcagt caggtgtcgg caaaaccttg ctctgtctgc
    15001 agtttctcgc agccggcctg gtgcgcggcg aaccctgcct gtatctgggg ttttatgaag
    15061 gcccgcagcg gctgatcggc aaggccgagg ccgtctcgat cgaactcgcc gacgcgtacc
    15121 acgacggccg cctcatgatt caatggcaac cggccataga actagcggtt gacgaactcg
    15181 cgacgatcgc gcttgccaca gtcaagcaga ccggcgtgac gcggatcgtg atcgacggcg
    15241 tggaaggctt tcgcgactcg gccctgcggc ccgaacgctt cggcctgtta ctgaacgcgc
    15301 tgcttcatca actgcgggaa gcgtgcgtca ccacgatcct gacggaagaa ctgccgcttt
    15361 acgccggccc cggtcacgcg aagagcgtgc gggtctccgc gttgaccgag aacctcgtgc
    15421 tcttgcgcta cgccgaaacg gaaacgggtt tgcgacgcat gatctccgtg gtcaagcagc
    15481 gcgaaagcgc gcacgacacg tcgctgcgcg aactcgtgat ttcgtcgcag ggcctggacg
    15541 taatcgacga tccggccgac acccccagcg tgtatccggc gccaggcctc gcggccgcgc
    15601 tgacaccacg acgagatacg tagccgctca tgaaaaccat cctggtcgtg gacgacgagt
    15661 tcgacatcct cacggtctgg cgactgctgc tggagcggca cggctatacc gtgctgacgg
    15721 cgtccaatgg ggccgccgca ctcgaacaga tccgcaagac ccggcccgac atcatcgtgt
    15781 cggactgcat gatgccgatc atgtcggggc tgcaactgtg cgccgcattg aacgacgacc
    15841 ccgatctgcg cgccattccg atcatcctgt gcagcgcggc cgcggatatc cccgcccagc
    15901 cgaatccgac catcgagtac gcacgcaagc cgctttcatt cgacagcctt ctcgcgatgc
    15961 tggagcgcat gacggtttga ctgactaggt aaaaccccga attcctggca agcgccacac
    16021 agactaatct cggcacattc attaaataga acatcattct gtccaacaga acgacgtaag
    16081 cgagagaccc tcgaaggaga attccatggc aagcctgccg ctcgacgcac ctggcgcgtc
    16141 gcccgattca agcgccggag cgcagggact cacgaccggc atcaacgcgc gcatcgaccg
    16201 tctgcccgcg acgcgcacag tatggatgct ggtgttcctg ctgtcgatcg gcggctggtt
    16261 cgagttctac gatctctttt ttacggcgta tgtcggtccc ggcctggtca agagcgggct
    16321 ctatgcgacg acgaccgcgt cgttcttcgg cgtctccggg ctgggcgcct tcgtggcggc
    16381 gtcgttcgcc gggctgttca tcggtacgtt ctttttcgcg ggcatggcgg atcgctatgg
    16441 ccgcaagacc gtgtttaccg tttcgctgct gtggtactcc gtggcgacgc tgatcatggc
    16501 gttgcaaacc accgcgccgg tcatcaacct gtggcgcctg atcgcgggca tcggcgtggg
    16561 cgtcgaactg gtgacgatcg acacttacgt cagcgaactc gtaccgaaac atctgcgcgg
    16621 ccgtgctttc gctttcgttc atctggtgca atacaccgcc gtgccgtcgg tcgccttcct
    16681 ggcgtggtgg ctggtgccgc aaagcccatt cggcctcgat ggctggcgct gggtcgtgat
    16741 catcggcgca ctgggcgcgg tcgtggtgtg ggccatccgg cgacgcgtgc cggaaagtcc
    16801 gcgttggctc gcgcagcaag gccgcgccgc cgaagcggaa aaagtcctgc aggcactcga
    16861 agcgaaaatc gcgagccaat acggcaagcc gctgcccccc gccgtgccga ctgtcgagcc
    16921 ggccaccacc aaggccgcct tccgcgaaat ctggcagccg ccgtatcgca aacgcgcgat
    16981 cacgatgctc gtgttcaacc tgttccagtc gatcggcttt tacggtttcg cgtcatgggt
    17041 gccgaccctg ctggtctcca aaggcgtgac gatcacgcac agcttgctgt actcgttcgt
    17101 gatcgcgatc tcgaatccgt tcggaccgct gatcggtatg gcgattgcag accgcatcga
    17161 acgcaagacg ctgatcgtgt tgtcggcgct cggtatcgcc gtgttcggca gcctgttcgc
    17221 aacgcaaacc tcgcccgcga tgctgatgac cctcggcgtg ctgatcacgc tctgcggcac
    17281 gctgctctcg gtcggctatc acgcgtacca gaccgaactg ttccccacgc gcttgcgggc
    17341 acgcgccgtg ggtttcgtgt actcgatgtc gcgtttgtcc gccatgtttt ccggcttcat
    17401 gatcgccttc gcgttgcgcc atttccaggt gccaggcgtg ttcgcgttga tcaccgtgtc
    17461 gatggtgatg gtgatgggcg cgatcggcat cttcggcccg cgtaccaaca accgcaatct
    17521 cgacgaaatt tcgcattgaa caagaggctt caaagatgac gctgccgttg gctggagtgc
    17581 gggtgctgga tctgtcgaat gtgttggcgg gaccgttttg cgcatatcaa ctcgcgctgc
    17641 tcggcgcgga ggtgatcaag gtcgagcacc ccgaaggcgg cgatcttgca cggcggttag
    17701 gcgccgacaa ggaagcctcg atgcgcaata tgggggcctc gttcgtcgcg gtgaacgcgg
    17761 gcaagcagtc catcacgctc aatctgaaag acccgcgcgg caagaccatt ttgcgggagc
    17821 tcgtcaaaac cgcggacgtg ctggtcgaaa attaccggcc tggcgtcatg acgcggctcg
    17881 gtctcgattt cgacgcgttg cgcgaggtca atccgaagct cgtctattgc gcgatctccg
    17941 gcttcggcat ggacggcgag ttttcgaaac gccccgccta cgatcagatc atccagggca
    18001 tctcgggtgt gatgagcgtg accggcgacg ccgatagcgc gccgttgcgc gtcggctatc
    18061 cggtatcgga cacggtgggc ggtctcacgg ctgcgttcgg catctgcgcc gcgctggtcg
    18121 atgcccgcgc gagcggacac ggccgcatgc tcgacgtttc gatgctggag gccacgctgg
    18181 cgacgatggg ctgggtggtg tcgaactatc tgaacgccgg cgtcacgccg gagccgatgg
    18241 gcaacgagaa cttcaccgcc gcgccttcgg gcacgttcaa aacgggcgac gggccgctca
    18301 atatcgccgc gaacgagaac aagcagttcg cgagtctctg ccatctgatc ggccgcgccg
    18361 atctgctcga cgacgcgcgt ttttccgagc gcaatgcacg caaagtaaat cgcgcggcat
    18421 tgaaggccga aatcgaagca gcgctgg
//
LOCUS       DATASH010000506         2639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1069980,
            whole genome shotgun sequence.
ACCESSION   DATASH010000506 DATASH010000000
VERSION     DATASH010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069980"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1287
                     /locus_tag="VHV99_14015"
     CDS             <1..1287
                     /locus_tag="VHV99_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEX3636576.1"
                     /translation="LADAADALAIAGEMPSEEDGDGDAAVEADALDDVLDADPDTDTD
                     TDADPDTDTDADPDADADADSKVEPSAQRKGRATAGGIESEVLEAFIAQHYIGNRVPP
                     VLVVSHAPATRELVDVLIEQAGHKVTVLRQPQGQRRAWLAMAEQNARLALARLLSEQG
                     SQQARTRALTDTLGMECDDLAHLRIECFDISHTMGEATQASCVVYHHHKMQSSEYRRY
                     NITGITPGDDYAAMRQVLTRRYEKMVAQAAANAADEAAELQTDASADPSIAPDAAEPV
                     AAGGILPTIVLIDGGKGQVEIARQVFTELGLDTGMLVGVAKGEGRKVGLETLIFADGR
                     APLELGKESAALMLVAQIRDEAHRFAITGMRAKRGKTRQTSRLEELEGVGAKRRQRLL
                     ARFGGLRGVVAASVEDLASVEGISQALAEQIYRQLH"
     gene            1396..1989
                     /gene="pgsA"
                     /locus_tag="VHV99_14020"
     CDS             1396..1989
                     /gene="pgsA"
                     /locus_tag="VHV99_14020"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEX3636577.1"
                     /translation="MPFNFPIFLTWLRIVLIPLVVGVFYLPDMMMSPAHRNLAAATIF
                     ILAALTDWFDGFLARKWNQTSAFGAFLDPVADKLMVTAALLVLVQLARIDSAIALVIV
                     GREIAISALREWMAQIGASKSVAVNSLGKFKTVCQMVAIPMLLFYGPLPFGSGVSVDT
                     RVWGLWLIYLAAFLTIWSMLYYMKLAWPQIRERSGVA"
     gene            2225..2300
                     /locus_tag="VHV99_14025"
     tRNA            2225..2300
                     /locus_tag="VHV99_14025"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2258..2260,aa:Gly,seq:gcc)
     gene            2405..2478
                     /locus_tag="VHV99_14030"
     tRNA            2405..2478
                     /locus_tag="VHV99_14030"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2437..2439,aa:Cys,seq:gca)
BASE COUNT          486 a          802 c          854 g          497 t
ORIGIN      
        1 ttggccgatg cggctgacgc gctcgccatt gccggggaga tgccctcaga agaagacggc
       61 gatggagacg ctgcggtcga ggcggatgcg ctggatgatg tgttggatgc cgaccccgac
      121 accgacaccg acaccgacgc cgaccccgac accgacaccg acgccgaccc cgacgccgac
      181 gccgacgccg actccaaagt cgaaccctcg gcgcaacgca aaggccgtgc gaccgccggc
      241 ggcatcgaat ccgaagtgct ggaagcgttc atcgcgcagc actacatcgg caatcgcgtg
      301 ccgccggtgc tcgtggtcag ccacgcgccg gctacgcgcg agctggtcga tgtgctgatc
      361 gagcaggccg gccacaaggt gaccgtgctg cgccagccgc aaggtcaacg gcgtgcatgg
      421 ctcgcgatgg ccgagcagaa cgcgcggctc gcgctcgcac gcctgctctc ggagcaaggc
      481 tcgcagcagg cccgcacacg cgcgctcacc gacacgctcg gcatggagtg cgacgacctc
      541 gcgcatctgc gtatcgagtg cttcgacatc agccacacga tgggcgaggc gacgcaggcg
      601 tcgtgcgtgg tgtatcacca tcacaagatg cagtcgtccg agtaccgccg ctacaacatc
      661 accggcatca cgccaggcga cgattacgcg gcgatgcgcc aggtgctcac gcgccgctac
      721 gagaagatgg tcgcgcaagc ggctgcgaac gccgcggacg aagccgccga attgcaaacc
      781 gatgcgtccg cggacccgtc catcgcaccg gatgcagcgg agccggtagc ggctggcggc
      841 atcctgccga ccatcgtgtt gatcgacggc ggtaagggcc aggtcgaaat cgcacgccag
      901 gtgttcaccg aactgggtct cgataccggc atgctggtcg gtgtcgcgaa gggcgagggc
      961 cgcaaggtcg gtctcgagac attgattttc gccgacggcc gcgcaccgct cgagctcggc
     1021 aaggaaagcg cggcgctgat gctggtcgcg caaatccgcg acgaggcgca ccgttttgcc
     1081 atcaccggca tgcgcgcgaa gcgcggcaag acacgccaga cctcgcgcct cgaagagctc
     1141 gaaggcgtag gagcgaagcg gcgccagcgg ctgttggcgc gctttggcgg cctgcgcggc
     1201 gtcgtcgcgg cgagcgtcga ggatctggcg agcgtcgaag ggatttcgca ggcgctggcc
     1261 gagcagatct accggcagtt gcactgatcc ggcgtccagg cggcgcccgt gcgaggcgcc
     1321 gcgcgccgct ttgcttgtgg caggccttgt gacacggcac aattgcatct cccttgtcag
     1381 acgctgcgcc tgcccatgcc gtttaatttc ccgattttcc tgacttggct gcggatcgtc
     1441 ctgattccgc tcgtcgtcgg cgtgttttat ttgccggaca tgatgatgag tccggcacac
     1501 cgcaatctgg cagctgcgac gattttcatt ctggcggcgc tgaccgactg gttcgacggc
     1561 tttctggccc gcaaatggaa tcagacctcg gcgttcggcg cgtttctcga tccagtcgcc
     1621 gacaagctga tggtgacggc tgcgttgctg gtgctcgtgc aactcgcgcg gatcgattcg
     1681 gcgattgcgc tggtgatcgt cggccgtgag atcgctatct cggcgctgcg cgaatggatg
     1741 gcgcagatcg gcgcatcgaa gagcgtcgcg gtgaattcgc tcggcaagtt caagaccgtg
     1801 tgccagatgg tggcgatccc gatgctgctg ttctatgggc cgttgccgtt cggtagcggt
     1861 gtctctgtcg acacgcgtgt gtggggtctg tggctgatct atctggccgc attcctgacg
     1921 atctggtcga tgctctatta catgaagctc gcgtggccac agatccgcga gcgaagtggc
     1981 gttgcctgat cgaagcatca tcgaggcgca aggtcagggc ggttgaagca tgccgaacct
     2041 gtccgaccgt ccgcgaagca tgagtgaaat cttttcagtt aaagacattc gcaaaagggg
     2101 ttgacacgtt tcagtcgttt ctccataatc tcgtttctcc gatgcgagca gtttgaagcg
     2161 cggtgcagta aagagagcag ttgcagtaac aaggcagcag ttagcagggc aaaagcagca
     2221 caatgcggga gtagctcagt tggtagagcg caaccttgcc aaggttgagg tcgcgagttc
     2281 gagcctcgtc tcccgctcca tacataagtg gaagcaacgc ttcccttttt gtttggtggg
     2341 ccggtccgta cggttaccca ccaaacaaat ctgagaatct gcagctaggt tccagtccgc
     2401 ttgcggcgcg atagcaaagc ggttatgcag cggcctgcaa agccgtttag gccggttcga
     2461 ctccggctcg cgcctccagt agttgatgta aagaaaagcc cagacttgtc ggggcttttt
     2521 gtttctccgc agcgcggcac tgaagcgtgt ccggctgggt gtcgttgcat ggccttcacg
     2581 cctgcatcgc ggcataatca ccgaactgag ccatttcgta tgcgcctatc gcgcaacgc
//
LOCUS       DATASH010000507         3805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1465310,
            whole genome shotgun sequence.
ACCESSION   DATASH010000507 DATASH010000000
VERSION     DATASH010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1465310"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /gene="prfB"
                     /locus_tag="VHV99_14035"
     CDS             <1..968
                     /gene="prfB"
                     /locus_tag="VHV99_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEX3636578.1"
                     /translation="DVWSDSKHAQALGREKKLLDDTVGKLTSLDNDLRDAQDLFDMAR
                     EEDDEETLLACESDAQGIEQRVADMEFRRMFANPADPNNAFLDIQAGAGGTEACDWAS
                     MLLRQYLRYCERKCFKTEVLEQTDGDVAGIKNATIKIEGEYAYGFLRTETGVHRLVRK
                     SPFDSSGGRHTSFSSVFVYPEIDDSIEVDINPADLRIDTYRASGAGGQHINKTDSAVR
                     ITHMPSGIVVQCQNDRSQHRNRAEAMAMLKSRLYEAEIRKRQAEQDKLEAGKTDVGWG
                     HQIRSYVLDNSRIKDLRTNVEISNTKSVLDGDLDPFISASLKQGV"
     gene            1091..2647
                     /gene="lysS"
                     /locus_tag="VHV99_14040"
     CDS             1091..2647
                     /gene="lysS"
                     /locus_tag="VHV99_14040"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010091959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEX3636579.1"
                     /translation="MTEPTQPDTAQPNAAQPHVAPEVDDNKIMAERREKLRELREQGV
                     AYPNDFRPTHHAADLQTQYAGSDKEALEANPLEVAIAGRMMLKRVMGKASFATVRDGS
                     GQIQFFITPADVGQETYDAFKKWDMGDIVAARGVLFRTNKGELSVRCTELRLLSKSLR
                     PLPDKFHGLADQEMKYRQRYVDLIVTPETRKTFVARTKAISSVRKFMADADFMEVETP
                     MLHPIPGGAAAKPFTTHHNALDMQMFLRIAPELYLKRLVVGGFERVFEINRNFRNEGV
                     SVRHNPEFTMIEFYAAYTDYKWVMDFTEQLIRQAAIDALGSATISYQGRELDLAKPFH
                     RLTISQAIQKYAPQYTDAQLADDAFLRTELKKFGVDASQPQFLNAGVGALQLALFEET
                     AESQLWEPTYIIDYPIEVSPLARASDKVAGITERFELFITGREIANGFSELNDPEDQA
                     ARFKKQVDQKDAGDEEAMFYDADYIRALEYGMPPAGGCGIGIDRLVMMLTDSPSIRDV
                     ILFPHLRRED"
     gene            2981..3697
                     /locus_tag="VHV99_14045"
     CDS             2981..3697
                     /locus_tag="VHV99_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEX3636580.1"
                     /translation="MDNPDTKSTQQRRIHPLVATAAGAVIVASLAATAAVTGLFPHAS
                     SSGAQTDQTQAAQVSTQPNTQPGVVDSAAPASPSAQQAAQQQAQPQAQQPQPAPAPAP
                     QQPQYAQQQQAQPAPQYAQQPSQPAYCSTCGTVVGISAVRQEGHGTGIGAVGGAVAGG
                     LVGNQFGGGGGRTAMTILGAVGGGVAGNSVEKHIRSTTSYSVRVRMENGKTRYFTYHE
                     APPFQEGQRVRVENGTLVAS"
BASE COUNT          811 a         1236 c         1131 g          627 t
ORIGIN      
        1 cggacgtctg gagcgactcg aagcacgccc aggcgctcgg ccgggaaaag aaactgctcg
       61 acgataccgt cggcaaactc acctcgctcg ataacgacct gcgcgatgct caagacctgt
      121 tcgacatggc ccgcgaagaa gacgacgagg aaaccctcct cgcctgcgaa tcagacgcgc
      181 aaggcatcga acaacgcgtc gccgacatgg aattccgccg gatgttcgcg aatccggccg
      241 acccgaacaa cgccttcctc gacattcaag ccggcgccgg cggcaccgaa gcgtgcgact
      301 gggcgtccat gctgctgcgc cagtatctgc gctactgcga gcgcaagtgc ttcaagaccg
      361 aagtgctgga acagaccgac ggcgacgtcg cgggcatcaa gaacgccacg atcaagatcg
      421 aaggcgaata cgcatatggc ttcttgcgca ccgaaaccgg cgtgcaccgc ctcgtgcgca
      481 agtcgccgtt cgattcgtcg ggcggccgcc atacgtcgtt ttcgtcggtg ttcgtgtacc
      541 cggaaatcga cgattcgatc gaagtcgaca tcaatccggc cgatctgcgc atcgacacgt
      601 accgcgcgtc cggcgcgggc ggtcagcaca tcaacaagac cgattcggcc gtgcggatca
      661 cgcacatgcc gtcgggcatc gtcgtgcagt gccagaacga ccgctcgcag caccgcaacc
      721 gtgccgaagc catggccatg ctgaaatcgc gcctgtacga agcggagatc cgcaaacgtc
      781 aggccgagca ggacaagctc gaagccggca agaccgatgt gggctggggt catcagatcc
      841 gctcgtacgt gctggacaac agccgtatca aggatctgcg caccaacgtc gaaatcagca
      901 ataccaagag cgtgctcgac ggcgacctcg atccgttcat cagcgccagc ctgaagcagg
      961 gcgtctaagc gcaaccggcg cggcatgcat cgcagcgctg cgccgcctct ttttaccgcc
     1021 tgaggttttt cactgactgg ccacccgcat gtggggcacc ccgatgcggg ccaccgaata
     1081 ccaaatcatc atgaccgaac cgacccagcc tgatacggcc cagccgaatg ccgcccagcc
     1141 ccatgtcgcg cccgaggtag acgacaacaa gatcatggcc gagcgccgtg aaaagctgcg
     1201 cgaactgcgt gagcaaggcg tcgcctaccc gaacgatttc cgtcccacgc accatgccgc
     1261 ggatctgcaa acgcagtacg ccgggagcga caaggaagca ctcgaagcca atccgctcga
     1321 agtcgcgatt gccggccgca tgatgctcaa gcgcgtgatg ggcaaggcca gctttgcgac
     1381 cgtgcgcgac ggttcgggtc agatccagtt cttcatcacg cccgccgacg tcggccagga
     1441 aacgtacgac gccttcaaga agtgggacat gggcgacatc gtcgcggcgc gcggcgtatt
     1501 gttccgcacc aacaagggcg aactctcggt gcgttgtacc gaactgcgtt tgctatcgaa
     1561 gtcgctgcgt ccgctgccgg acaagttcca tggtctcgcc gatcaggaga tgaagtatcg
     1621 ccagcgctat gtcgatctga tcgtcacgcc ggaaacgcgc aagactttcg tcgcacgtac
     1681 caaggcgatt tcgtcggtcc gcaagttcat ggcggatgcc gacttcatgg aagtggaaac
     1741 gccgatgctg cacccgattc cgggcggcgc cgcggccaag ccgttcacca cgcaccacaa
     1801 cgcgctcgac atgcaaatgt tcctgcgcat cgcgccggaa ctgtatctga agcggcttgt
     1861 ggtcggcggc ttcgaacgcg tgttcgagat caaccggaat ttccgcaatg agggcgtgtc
     1921 cgtgcgccac aatccggaat tcacgatgat cgagttctac gcggcgtaca ccgattacaa
     1981 gtgggtaatg gacttcaccg aacagttgat ccggcaggcc gcgatcgacg cactcggcag
     2041 cgccacgatc agctaccagg gtcgcgagct ggatctggcc aagccattcc atcgcctgac
     2101 gatcagccag gcgatccaga agtacgcgcc gcagtacacc gacgcgcaac tcgccgacga
     2161 cgcattcctg cgcaccgaac tgaagaagtt cggcgtggac gcgtcgcagc cgcagttcct
     2221 gaacgcaggc gtgggcgcgc tgcaactggc gctgttcgaa gagaccgccg agtcgcagct
     2281 gtgggagccg acctacatca tcgactaccc gatcgaagtg tcgccgctgg cgcgcgcgtc
     2341 ggacaaggtc gccggcatca ccgagcgttt cgaactgttc atcacgggcc gtgaaatcgc
     2401 caacggtttt tcggagctga acgatccgga agaccaggcc gcgcgcttca agaagcaggt
     2461 cgaccagaaa gacgctggcg acgaagaagc catgttttac gacgccgact acatccgtgc
     2521 gctggaatac ggcatgccgc cggccggcgg ttgcggtatc ggcatcgacc gtctggtgat
     2581 gatgctgacc gacagcccga gcatccgcga tgtgattctg ttcccgcatc tgcgccgcga
     2641 agactgatcg ccgcgtcgct cgagtccacg gctcgcaaag ggaaaacgcc cggctcaaga
     2701 tgccgggcgt tttttcttgc aagccctgaa aagcaccgtc tgctgtcacc tgacggtttg
     2761 taaccatcgg taaaaggtgc gtatcggcca cccggcgacc gattgcctgg cttgccgcgt
     2821 gcccctcgga aaatcagccg ccgcgccgcg cttcacgcgc tttgacgcga cctgcccgcg
     2881 atgattgccc ggaaatggtt acaaattgaa accctgctgg gatcgatttg cctgacactg
     2941 ggtctcaata aaactgactc tgctccagac ggaggccaat atggacaatc ccgataccaa
     3001 atccacgcaa caacgtcgta ttcacccgct cgtagccacg gctgcgggcg cagttattgt
     3061 tgcgagtctc gcggctacgg ctgccgtcac cggcctgttt ccccatgcgt cgagtagcgg
     3121 cgcgcaaacg gatcagacgc aggccgcgca agtgtccacg caaccgaaca cccagccggg
     3181 cgtggtcgac tccgcagccc cggccagccc ttcggcgcag caggccgcgc aacagcaagc
     3241 ccaaccacag gctcagcagc cgcaaccagc ccctgccccg gcaccgcaac aaccacagta
     3301 tgcgcagcaa caacaagccc aacccgcgcc gcaatacgcc cagcagcctt cgcagccggc
     3361 ctactgttcg acatgcggca cggttgtggg catctcggcg gtccgtcagg aaggccatgg
     3421 caccggtatc ggcgcagtcg gcggcgcagt ggccggcggc ctggtcggca atcagttcgg
     3481 cggcggaggc ggccgcactg caatgacgat ccttggcgca gtgggtggcg gtgttgcggg
     3541 taactcggtc gaaaaacaca tccgcagcac gacgtcgtat tcggtgcggg tacggatgga
     3601 aaacggcaag acgcggtact tcacgtacca cgaagcaccg ccgttccagg aaggtcagcg
     3661 cgtgcgggtc gagaacggca cgctggtggc gagctgatcc agacggaccc actgaagcca
     3721 aacgcagcct gaacattttg caggcgcaaa aaaggctatt ccaatggact cgcctttttc
     3781 tttgcctgcg gttcttcgcg ctacg
//
LOCUS       DATASH010000508         6393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_7003801,
            whole genome shotgun sequence.
ACCESSION   DATASH010000508 DATASH010000000
VERSION     DATASH010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6393
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7003801"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            307..768
                     /locus_tag="VHV99_14050"
     CDS             307..768
                     /locus_tag="VHV99_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3636581.1"
                     /translation="MNEPYQRPSLGASVYYKDPFAALDWLEKAFGFERQMVITDSDGQ
                     LGHSEMRFGDSYVMVCREWSEDAASPASIGGKNTQSVHVQLREGIDEHCARARAAGAV
                     ITREPADQFYGDRVYAARDPEGHVWSFGQTVREVSREEAERVSGLKIEGWA"
     gene            772..1110
                     /locus_tag="VHV99_14055"
     CDS             772..1110
                     /locus_tag="VHV99_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX3636582.1"
                     /translation="MGLPASVSLDRTLAALADPNRRQVVDLLSQQPMRAGELAQATGL
                     SPQAMSRHLRVLRSSELIEESHDGVDARVRLYVLRSAPMSELKAWLEQTEALWSEQLL
                     SFKAHLEQQA"
     gene            1107..1607
                     /locus_tag="VHV99_14060"
     CDS             1107..1607
                     /locus_tag="VHV99_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX3636583.1"
                     /translation="MSSRVQVSLRVAATPRRAFDVFTREIGRWWRPNGLFQFTPQGAG
                     VLSFASSSASGELGRLVETQPDGSVFEIGRVTAWEPGERLAFGWRQASFTDAQHTHVE
                     VRFEAVGDETRVTVEHRGWDTVPQEHVARHHFPERVFLLRHGEWWQALLASLRIEVQA
                     AVVPPG"
     gene            complement(1738..2349)
                     /locus_tag="VHV99_14065"
     CDS             complement(1738..2349)
                     /locus_tag="VHV99_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEX3636584.1"
                     /translation="MSLSALLVFALALIIAAGTPGPSVAALVARVLTNGFRDVLPFLA
                     AMWLGEALWLTCAVAGLAVIARSFGMVFIALKFVGVAYLLFLAWKMWRAPADVEGRDL
                     PSGQSPWRMFVAGLLVTLGNPKIMMFYLALLPTIIDLSRIGTVAWFELTLTMLIVLMA
                     VDFGWALLATRARKLLSTRRAVRITNRASATVMAGVAAAIATR"
     gene            complement(2550..3197)
                     /locus_tag="VHV99_14070"
     CDS             complement(2550..3197)
                     /locus_tag="VHV99_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX3636585.1"
                     /translation="MDLMHVLQVALHFDQHLSGLIVQYGTAVYAMLFLVVFVEIGFLP
                     LFFLPGDPLIFICGGLAATGALNVWLVIPVLFAATVAGSIVDYAIGRAVGEKVYTADY
                     RWLDKHALRKAHAFYEARGGLTFLLSPFIAVVRTFAPFVAGVSRMTFARFVSFVTAGA
                     ALWIVSLVAAGYLFGNVPLVRDHMSSIVLLGVSLGVGSLLMSAVWRFVNRRLRAH"
     gene            3334..3861
                     /locus_tag="VHV99_14075"
     CDS             3334..3861
                     /locus_tag="VHV99_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3636586.1"
                     /translation="MQHASSLTFRLAALHDADAIRAIEFEAGQRFVSVGLTGIADAPP
                     MELELVNRKIGAREIVVAVDADGTCVGFVMFEPQPARFYVQELDVLTSHAGQRIGAAL
                     IEQVAQLARAQQIMQLILSTFREVPWNAPYYRRLGFRDMEQADLDAVLIARRDAHIAR
                     GLDESKRVFMRRDLA"
     gene            complement(4062..5123)
                     /locus_tag="VHV99_14080"
     CDS             complement(4062..5123)
                     /locus_tag="VHV99_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HEX3636587.1"
                     /translation="MPTLFDPLQIGDITLSNRIIMAPLTRQRAEEIRVPNALMAKYYA
                     ERATAGLIISEATSVTPQGVGYADTPGIWSQEQVEGWKLVTNAVHAAGGKIFLQLWHV
                     GRISDPLFLNGELPVAPSAIAAQGHVSLVRPERPYVTPRALELDEIAGVVDAFRKGAE
                     NAKAAGFDGVEVHGANGYLLDQFLQDSTNTRTDAYGGPIENRARLLLEITDACIGVWG
                     ANRVGVHLAPRRDAHAMGDSDPAGTFGYVARELGKRKIAFIAAREALGDDRLGPQLKK
                     AFGGPYIANEKFTRETAQQVLDAGEADAVAWGQLFIANPDLVRRFETNAPLNKPNPAT
                     YYARGEVGYVDYPTLETVE"
     gene            complement(5226..5528)
                     /locus_tag="VHV99_14085"
     CDS             complement(5226..5528)
                     /locus_tag="VHV99_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015877514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX3636588.1"
                     /translation="MTLDIDAIHKALANPVRREILGWLREPYAHFSDQDLPLDHGVCA
                     GKIDARCGLSQSTVSAHLAALQRAGLVTSKRVGQWVFFKRNEAVIQAFLEHMNTGL"
BASE COUNT         1277 a         1863 c         2035 g         1218 t
ORIGIN      
        1 cactgtttga agaaactcag gctcatgcga ggaaacacga tgctggacgg tctatgaatc
       61 tatccgtgtc gtgaagtaat tgcggagacc gcgttgagga tatctggcag tagttaaccc
      121 gaaacggtca gggacattac aaatttgcca tgcggcatct ttttaatccg aaaccggccg
      181 tccgcttatt gcttttcttt ttcaactttt tgtcggcgaa cacagggtgg actccgtcct
      241 tgccttttcg acttgtttaa ctatagtgtt aaatatcaac cggatggttt atagaaggag
      301 gcaaccgtga acgaaccgta tcagcgtcct tcgctgggtg cgtcggtgta ctacaaggac
      361 ccgtttgccg cgctcgattg gctggagaaa gcgttcggct tcgagcgtca gatggtcatc
      421 acggatagcg acggccagtt gggccactcg gaaatgcgtt tcggcgacag ctacgtgatg
      481 gtctgccggg aatggtcgga agacgccgcg agcccggctt cgatcggcgg caagaatacg
      541 caatcggtgc acgtgcaact gcgcgagggc atcgacgaac attgcgcccg tgcgcgcgcc
      601 gcgggcgccg tgattacgcg cgagccggcg gatcagtttt acggcgaccg cgtttacgcg
      661 gcgcgcgatc cggaagggca cgtctggagc ttcggtcaga cggtgcgcga agtgtcgcgc
      721 gaggaagccg aacgcgtgag cggcctgaaa atcgaaggat gggcgtaagc gatgggactg
      781 ccagccagcg tctcgctcga ccgcacgttg gccgcgctgg ccgatccgaa ccggcgtcag
      841 gtggtcgatc tgctcagcca gcagccgatg cgcgcgggcg aactcgcgca agccaccggc
      901 ctctcgccgc aggcaatgag ccggcatttg cgggtgctgc gctcgagcga actgatcgaa
      961 gaatcgcacg acggggtcga cgcgcgcgtg cgtctctacg tgctgagatc ggcgccgatg
     1021 agcgaactga aggcgtggct cgaacagacc gaagccttgt ggtccgagca attgctgtcg
     1081 ttcaaggcgc atctggagca gcaggcatga gttcacgcgt gcaggtgtcg ctacgcgtcg
     1141 cggccacgcc gcggcgcgcg ttcgacgtgt tcacgcgcga gatcggcaga tggtggcggc
     1201 caaacgggct gtttcagttc acgccgcaag gcgcgggcgt actgtcgttc gcgtcttcgt
     1261 cagcgtccgg agaactcgga cgcctcgtcg aaacacagcc ggacggcagc gtgttcgaga
     1321 tcggcagagt cacggcctgg gagccgggcg agcgtctggc attcggctgg cgtcaggcca
     1381 gtttcaccga tgcgcagcac acgcatgtcg aggtgcgttt cgaagcggtg ggcgacgaaa
     1441 cgcgcgtgac agttgagcat cgcggctggg atacggtgcc gcaagaacat gtggcgcgcc
     1501 accatttccc cgagcgtgta tttctgctgc ggcacggtga atggtggcag gctttgctgg
     1561 cgtctttacg tattgaagtg caagccgcgg ttgtgccgcc aggctgaaaa acgggcgagc
     1621 ttacagtcag cgtgtggtga tagtcgctgc gccgccaggc tgaaaagcaa gcgaatttac
     1681 ggtcagttgt ggcgatagcc actacgccgc cagaccgcca ggctgacgag tcggcgctca
     1741 gcgtgtcgca atagcggcgg ccacaccggc catgaccgta gcgctcgcgc gattggtgat
     1801 cctgacggct cggcgggtgc tcaacagctt gcgcgcacgt gtggcgagca gggcccagcc
     1861 gaagtcgacg gccatcagca cgatcagcat ggtgagcgtc agttcgaacc acgcgacggt
     1921 gccgatgcgc gacagatcga tgatggtcgg cagcaacgcg aggtagaaca tcatgatctt
     1981 cgggttgccg agtgtgacca gcaggccggc gacgaacatg cgccacggcg actggccgct
     2041 cggcaagtcg cggccttcta cgtcggccgg cgcgcgccac attttccatg caaggaacag
     2101 cagataggcg acgccgacga atttcagcgc gatgaacacc atgccgaagc tgcgcgcgat
     2161 caccgcgagg ccggccaccg cgcaggtgag ccacaatgct tcgccgagcc acatcgcggc
     2221 gagaaacggc agtacgtcac gaaagccgtt ggtcagcacc cgcgcgacca gcgcggcgac
     2281 gctggggccg ggcgtaccgg cggcgatgat cagcgccagc gcgaagacga gcaaagcaga
     2341 tagcgacata acaatctcca cgaagttcgg tgtgcgcgca aaccttcggt ttagtgcgta
     2401 cgcaaccacc gctcagcgcg ctcgcaacct gcgactcagc gcgctcgcaa caggcgactc
     2461 agcgagtccg caaccggcgg ttcagcgcgc ccgcaaccgg cgactcagcg agtccgcaac
     2521 cggcggttca gcgcgcccgc aactaccgct cagtgagccc gcaacctccg gttgacgaag
     2581 cgccacacgg cgctcatcaa caacgatccg acaccgagcg agacacccag cagcacaatc
     2641 gaactcatat gatcgcgtac cagcggcacg ttgccaaaca gatagccggc cgccaccagc
     2701 gacacgatcc acagcgccgc cccggccgtg acgaacgaca cgaaacgcgc gaacgtcatg
     2761 cgcgaaacgc cggcgacgaa cggcgcgaaa gtgcgcacga cggcaatgaa cggcgacagc
     2821 aaaaaggtca ggccgccgcg tgcctcgtaa aacgcatgcg ctttacgcag cgcatgcttg
     2881 tcgagccagc ggtagtcggc cgtgtagact ttttcgccga ccgcgcggcc gattgcatag
     2941 tcgacgatgc tgcccgccac cgtggcggcg aacagcacgg gaatcacgag ccagacattc
     3001 agcgcgccgg tcgcggcaag accgccgcag atgaagatca ggggatcgcc aggcaaaaag
     3061 aagagcggca gaaagcctat ctccacgaac acgacgagaa acagcatcgc gtagacggcg
     3121 gtgccgtatt gcacgatcag gccgctcagg tgctggtcga agtgcagggc gacctgcagc
     3181 acatgcatga ggtccatctt ggttcgaaag ggattggggt gttcgggagt ttagtcccac
     3241 ggcggcgcgg gcacttgttt atttcgtggc gttaatgtca acttcgggct gtgtcctccg
     3301 aaatcgcgtt caaaagaacg ctacgaaacg cccatgcaac atgcttccag tctcacgttc
     3361 cgccttgccg cgctgcacga tgcggacgct atccgtgcga tcgaattcga ggcgggccag
     3421 cgcttcgtca gcgtcggctt gacaggtatc gccgatgcgc cgccgatgga gttggaactc
     3481 gtcaaccgca agatcggggc gcgggagatt gtcgtcgccg tggatgccga tgggacctgc
     3541 gtggggtttg tgatgttcga gccgcagccc gcgcgttttt atgtgcagga gctcgatgta
     3601 ctcacgtcgc atgccgggca acgtatcggc gcggcactga tcgagcaggt cgcgcaactc
     3661 gcgcgtgcgc aacagatcat gcaacttatc ttgtcgacgt ttcgcgaggt gccgtggaac
     3721 gcaccgtact atcgacggct cggctttcgc gatatggagc aggccgatct cgacgccgtg
     3781 ctgatcgccc ggcgcgatgc gcatatcgcg cgaggcctcg acgaatcgaa gcgcgtgttc
     3841 atgcggcgtg atctggcatg acggcagaca gcagacgtga gcgcctggat cgctcgtagt
     3901 gcgggcagtg cacgcgctgt acgcaacggc taacgcatga aaaaacgcgg cgggcgtagc
     3961 cggacatcgt tccggcccta cccgccgcgt ggtcttgcag cctactacga gcaatcagcc
     4021 gctttaacga agcggcttaa gcccgggctg acttcaactg cttattccac cgtttccagc
     4081 gtcggatagt ccacgtagcc gacttcgccg cgtgcatagt acgtggccgg attcggcttg
     4141 ttcaacggcg cattcgtctc gaagcggcgc accagatccg gattcgcgat gaacagctga
     4201 ccccacgcca ccgcgtccgc ttcaccggcg tcgagtactt gctgcgcggt ttccctggtg
     4261 aacttttcgt tcgcgatgta ggggccgccg aatgccttct tcagttgcgg gccgaggcgg
     4321 tcgtcgccga gcgcttcgcg ggcggcgatg aacgcaatct tgcgcttgcc gagttcgcgt
     4381 gccacataac caaaagtacc cgccggatcc gaatcgccca tggcgtgtgc gtcgcggcgc
     4441 ggtgcgaggt gaacgccgac acggttcgcg ccccagacgc cgatacacgc gtcggtgatt
     4501 tcgagcagca ggcgcgcgcg gttttcgatc gggccgccgt aagcgtcggt acgcgtgttg
     4561 gtgctgtcct gcaggaactg gtcgagcaga taaccgttcg cgccgtgcac ttccacgccg
     4621 tcaaaacccg cggccttggc gttttcggcg cctttgcgga atgcgtcgac cacaccggcg
     4681 atttcgtcga gttcgagcgc gcgcggcgtc acgtacggac gctctggacg caccaggctc
     4741 acgtggcctt gtgccgcgat ggcgctcggc gcgaccggca gttcgccgtt caggaacagc
     4801 gggtccgaaa tacggccgac gtgccacagt tgcaggaaga tcttgccgcc ggctgcatgc
     4861 acggcgttcg tgacgagctt ccagccttca acctgttctt gcgaccagat gcccggcgtg
     4921 tcggcgtaac cgacaccttg cggcgtgacc gaagtggctt cgctgatgat caggcccgcc
     4981 gtggcgcgtt cggcgtagta cttcgccatc agcgcgttcg gcacgcggat ttcttcggcg
     5041 cgttgacgcg tgagcggcgc catgatgatg cggttggaca acgtgatgtc gccaatctgg
     5101 agcgggtcaa aaagagtcgg catatgagtt cacctcaggt ggcgggcgcg tggcccgagc
     5161 cttaaaaaac tgcgcaatga aataagcgaa ataagcaaaa aaacgcggcg atgcggatga
     5221 agcgctcaga gcccggtatt catgtgctcg agaaaggcct gaatgacggc ctcgttgcgt
     5281 ttgaaaaaca cccattgacc aacccgcttc gacgtgacga gacccgcgcg ctgcaaggcg
     5341 gcaaggtgcg ccgacacggt ggactgcgac aggccgcagc gcgcgtcgat cttgccggcg
     5401 cacacgccgt gatcgagcgg caggtcctgg tcggagaaat gcgcgtacgg ctcgcgcaac
     5461 cagccaagaa tctccctgcg cacagggttg gcaagcgctt tgtgaatcgc gtcgatatcg
     5521 agcgtcatgt ggatcggttt ttgggtcatc aacaacaggc gggcgtgccg ccggctagcc
     5581 gcccggcagc gccccgcatc tgcatcgcta caagacgaat catatatcgt aatttaacga
     5641 tatgtgtgaa ggcactactg gcgatggggt cgataggtgt ccgcgcacaa tatagtttgt
     5701 acttttttgt ttggtacagg aacgcacaaa agagattgtg tttcttgttt ttcatcatgg
     5761 aaaattggat ggaacttacg gatttttaac ggattttgtt gtcgatctaa tatctcttgc
     5821 aaggcgaggt ttgtggccgc atatgctggc taggtttgtt tataaacttc ggattgctga
     5881 atgtatgttt gcacttcgcc ggcgctgcaa cgggttccgt taatctttcg atttgctttc
     5941 gagatgaggg gcaatgctgc atgcgttggc cgtctcgtca acttctcggt cgaactggaa
     6001 agacggcgtt ctttcatgag ccgctaaaac ggaggcgaac aggggtatgt gcgatgagcg
     6061 tcgatcaatc ggtgcctgcg gatattgcat tgttcaatcg aaggggctcg caaatcgggc
     6121 attgccgaca ctggggcaac gaagtctgca aggcggatgt aagtcaatct cctgccgttc
     6181 gcacatcgac gaccgccaat ctcaagtttg gacgtcggcg aacgcttgct cccgaccgat
     6241 actgtttgca aggacttccc ctgaaagtca aggtcggggt tcgatttttt tcgaccctcg
     6301 cagatattgg cgcgcacttg caggcggccc tgttggtgtg gtcatagcat ttttcctcaa
     6361 gtaggctgcc gctgtcgtgt cgaagcaata cca
//
LOCUS       DATASH010000509         3652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6805365,
            whole genome shotgun sequence.
ACCESSION   DATASH010000509 DATASH010000000
VERSION     DATASH010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6805365"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VHV99_14090"
     CDS             <1..553
                     /locus_tag="VHV99_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfoacetaldehyde dehydrogenase"
                     /protein_id="HEX3636589.1"
                     /translation="AALWSNGKLSAHCTARSAASIARAAGLNDIAARGPAFLMVEESG
                     FGADYPFSGEKLAPVLTVYRADSFTAAADIVRGIYAYMGAGHSVGLHSSDPEAAVQLG
                     STLPVARVIVNQAHCLATGGNFDNGLPFSLSMGCGTWGRNNFSSNLNFHHYLNITRVA
                     YPIEERVPELDDVLGDFFARYGK"
     gene            550..2208
                     /locus_tag="VHV99_14095"
     CDS             550..2208
                     /locus_tag="VHV99_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX3636590.1"
                     /translation="MKMPHSIRALIDARAAQYPDKPFLLTASESATDGDAAAHADVLT
                     FAALRDDCRALETVFHEAGLQAGDVVSVFMGNGIHTARLLLAAMYSGLVANPLNLLCQ
                     PSQLRYIVEHSDTRMVFVSGDTHAAMASALAELREQGVTRTIALIRTEPDAAVVRLPA
                     CVEPALVETAGVAAAHRVSANDALHAPSLSAWDSQVSSSAEPDASDVALLMYTSGTTG
                     APKGVLLDHRNLLANARNISQEHRLGSGDRVFAALPLYHINGLVVTLLAPLFHGGSVV
                     MSPRFAARTYWRDVARHGCTWINVVPTIVAYLLNNDEPCSFDLSALRFCRSASAALPA
                     DHHRAFEARFGVGIIETMGMTETAAPVFSNPYDPERRRIGSIGLPSGGEAKVIDRDGR
                     ECAANECGELVLRGEQVMRGYYKRAEETHAAFTADGWLRTGDLGYRDSDGYFYINGRA
                     KELIIKGGENIAPREIDEALLKHPGVLDAAVVGVPDSAYGQEIVAFIVPRIGAGQGAL
                     DVADLRAYCLRELGRYKTPKEFRFVAELPRGPSGKVQRLKLVPT"
     gene            2351..>3652
                     /locus_tag="VHV99_14100"
     CDS             2351..>3652
                     /locus_tag="VHV99_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012405420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3636591.1"
                     /translation="MQTHKRRVKTRHIILAVMCLMYFISYIDRVNIAVAGPIIRHEMG
                     LTTVQLGLVFSAFAYPYAFMQVVGGWLSDKYGPKLVLTVLSLIWGAATLATGFAGSVA
                     MLVLLRFALGIGEGGAFPTATRAFTYWMPVAERGFAQGITHSFARLGGAVTPPLVLAV
                     VVAGGWRDAFILLGVASLAWTALYLCVFTNSPEQNRRVTPEETAEIGYRAGDCERAKR
                     AATPWRKLVRRMWLVTFVDFCYGWLLWVYLTWLPSYLKEARGFDLKQLALFTALPLLA
                     GVIGDTLGGVVSDRLYKRTGRLRFARCAVLVVGMGGSLVFLLPMVSATNPLMAVVFLS
                     ASFFFLEITNPVLWTLPLDIGGRYAGTAGGMMNTGFGVAGMISPVVFGYLIETTGSYN
                     VPFTISAGLLAVGIVGALFIDPNRTVEADEERERLEGRQLDD"
BASE COUNT          585 a         1148 c         1212 g          707 t
ORIGIN      
        1 ggcggcccta tggtcgaacg gcaagctctc ggcgcactgc accgcgcgct cggcggcgtc
       61 gattgcacgg gcggcaggac tcaacgacat tgccgcgcgc ggacccgcct ttctgatggt
      121 ggaggaaagc ggcttcggcg ctgactaccc gttctcgggc gaaaagctcg caccggtgct
      181 gacggtttat cgcgcggaca gcttcacagc cgccgccgat atcgtgcgcg gcatctacgc
      241 ctacatgggc gccgggcact cggtcggcct gcattcctcc gaccccgagg cggcggtgca
      301 gctcggttca acgctgccgg tggcgcgcgt gatcgtgaat caggcgcatt gcctcgcgac
      361 cggcggcaac ttcgataacg gcttgccctt ttcgctgtcg atgggatgcg gaacctgggg
      421 ccgcaacaat ttctcgagca atctcaactt tcaccattac ctgaacatca cgcgcgtggc
      481 ttatccgatc gaggaacgcg tccccgaact ggatgacgta ctcggggatt tcttcgcgag
      541 gtatggcaaa tgaagatgcc tcactccatc cgcgcgctga tcgacgcgcg cgccgcgcag
      601 tatcccgaca agccgttcct gctgacagct tctgaaagcg caacggacgg cgacgctgcc
      661 gcgcatgccg acgtgttgac ctttgcggcg ctgcgcgacg actgccgcgc gctcgagaca
      721 gtctttcacg aagcaggctt gcaggccggc gatgtggttt cggttttcat gggcaacggc
      781 attcatacag cgaggctgct gctcgcggcg atgtacagcg ggctcgtcgc caatccgctc
      841 aatctgttgt gccagccgtc gcagctacgc tacatcgtgg agcattcgga cacgcgcatg
      901 gtctttgttt ccggcgatac gcatgcggcg atggcgagcg cgctcgcgga gctgcgcgag
      961 caaggtgtca ctcgcaccat tgcgctgatt cgcaccgagc ccgatgccgc cgttgtgcgt
     1021 ttgccggctt gcgtcgaacc cgcgctggtg gaaacggcag gcgtcgctgc cgcgcatagg
     1081 gtatcggcaa atgatgcgct ccatgcgcca tctttatccg cctgggattc ccaggtgtcg
     1141 tcaagcgccg agccggacgc aagcgatgtg gcgctgctga tgtacacgtc cggcaccacg
     1201 ggcgccccga agggcgttct gctggatcat cgaaatctgc tggccaatgc gcgcaacatt
     1261 tcgcaagagc accgcctcgg cagcggcgac cgcgtgttcg ccgccctgcc gctttaccac
     1321 atcaatggtc tcgtcgtgac gctgcttgcg ccgctctttc atggcggatc ggtggtcatg
     1381 tcaccgcggt ttgccgcgcg cacctactgg cgcgatgtcg cgcgccacgg ttgcacatgg
     1441 atcaacgtgg tgccgacgat cgtcgcctat ctgctcaaca acgacgaacc gtgctcgttc
     1501 gatctgtccg cgctgaggtt ctgccgcagt gcgtcggcgg cgttgcccgc cgatcaccat
     1561 cgcgcgttcg aggcgcgctt cggggtcggc atcatcgaaa cgatgggcat gaccgaaacc
     1621 gccgcgccgg tgttcagcaa tccctacgat cccgagcggc gacgaatcgg cagcatcggc
     1681 ctgccctccg gcggtgaagc gaaggtgatc gaccgtgacg gccgcgaatg cgcggccaac
     1741 gagtgcggcg aactggtgct gcggggcgaa caggtcatgc gcggctatta caagcgcgct
     1801 gaggaaacgc atgcggcctt caccgccgat ggctggctgc gcaccggcga cctcggctat
     1861 cgggacagcg acggttactt ctacatcaat ggccgcgcga aggaattgat catcaagggc
     1921 ggcgaaaaca ttgcgccgcg cgagatcgat gaagcgttgc tcaaacaccc cggcgtgctg
     1981 gatgcggcgg tggtgggcgt gccggacagc gcctacggac aggagatcgt cgccttcatc
     2041 gtgcctcgta ttggcgcggg gcagggtgca ctcgacgtcg cggatctgcg tgcgtactgt
     2101 ctgcgcgaac tgggccgcta caagacgccg aaggagtttc gcttcgtcgc ggaattgccg
     2161 cgtggacctt cgggcaaggt gcagcgcctg aagcttgtgc ccacctgacg gtatgcgcat
     2221 cggcgcgccc ggatcgcgtg gcacgcctag gaggaaggcg cgcaatgccc gcaatgcgcc
     2281 gcagtaacgg acgtacggat cgaacgacca cagacgccat cggcgtcaca taaaagacac
     2341 cggagacagt atgcaaacgc acaagcggcg tgtgaagacg cgccatatca tcctggccgt
     2401 catgtgcctg atgtatttca tctcgtacat cgatcgtgtg aatattgccg tggccggtcc
     2461 gatcatccgg cacgaaatgg ggctgacgac ggttcagctc ggcctcgtgt tctcggcctt
     2521 tgcttatccg tatgcgttca tgcaggtcgt cggcggctgg ctgtcggaca agtacgggcc
     2581 gaagctggtg ctgacggtgt tgtcgctgat ctggggcgcg gcgacgctcg ccaccggctt
     2641 cgccggcagc gtggccatgc tcgtcctgct gcggttcgcg ctgggcatcg gcgagggcgg
     2701 cgccttcccg accgcgacgc gtgcctttac ctattggatg ccggtggcag agcgtggttt
     2761 cgcgcaaggc atcacccata gcttcgcccg actcggcggc gcggtcacgc cgccgctcgt
     2821 gctcgcggtg gtggtggccg gcggctggcg cgacgcgttc attctgctcg gcgtggcgag
     2881 tctcgcgtgg acggcgctgt acctctgcgt gtttacgaac tcgccggaac agaaccgccg
     2941 cgtgacgccc gaggaaaccg ctgagatcgg ctatcgcgcc ggcgactgcg aacgcgccaa
     3001 gcgcgcggcc acgccgtggc gcaaactggt ccgccggatg tggctcgtca cgttcgtcga
     3061 cttctgttat ggctggctgc tgtgggtcta tctgacctgg ctgccttcgt atctgaagga
     3121 ggcgcgcggc ttcgatctca agcaactggc gctgttcacg gcgctgccgc tgctcgcggg
     3181 cgtgatcggc gataccctcg gcggcgtcgt ctcggaccgg ctctacaagc gcaccggccg
     3241 gctgcgtttt gcgcgctgcg cggtgctggt ggtgggcatg ggcggctcgc tcgtattcct
     3301 gctgccgatg gtgtccgcaa ccaatccgct gatggcggtg gtgttcctgt cggcgtcgtt
     3361 cttctttctc gagatcacca atcctgtgtt atggaccttg ccgctcgaca tcggcgggcg
     3421 ttatgcgggc acggcgggcg gcatgatgaa tacgggcttc ggcgtcgccg gcatgatctc
     3481 gccggtggtg ttcggctatc tgatcgaaac aacgggcagc tacaacgtgc cgttcacgat
     3541 ttccgccggt ttgctggcgg tggggatcgt gggcgcgttg ttcatcgatc cgaaccgcac
     3601 ggtcgaagcc gacgaggagc gcgaacggct cgaaggccgg caactggacg ac
//
LOCUS       DATASH010000510         2692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5026773,
            whole genome shotgun sequence.
ACCESSION   DATASH010000510 DATASH010000000
VERSION     DATASH010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5026773"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="VHV99_14105"
     CDS             <1..710
                     /locus_tag="VHV99_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3636592.1"
                     /translation="ASRGIGRASALALAKAGAQVLVHYSSGEKAADGVVAEIRAAGGN
                     AQIIAADLRDADGPHTLAKRVRAVVGHRLDILVANAGISKAASIEETTVGDFDELFAV
                     NVRAPYFLVQQLLPVMCKGSSVVLLSSLAAHAAVGKLPAYAATKGAVDTLVKHFASAL
                     GEREIRVNAVAPGVVETDMSNFTRTDAGRELALGMQALKRVAQPDDIGEAVAFLASDA
                     ARWITGETLRVDGGSKL"
     gene            946..1686
                     /locus_tag="VHV99_14110"
     CDS             946..1686
                     /locus_tag="VHV99_14110"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HEX3636593.1"
                     /translation="MSKLAQKVALITGGSRGIGAAIAKRLAADGARVAFTYAKDASAA
                     SAVVKEIELNGGKALAIQADATDVKAVEAAVEKTFATFGRLDVLVNNAGTAIPKPFED
                     TTLEEMDRVIDINIRGVFATTKAALKHLGDGGRIIMIGSAVGERAIAPGLVPYAATKA
                     AVRMFTQALSRELGGRGITVNNVQPGPIDTDLNPASGDWAVPQKAATVLGRYGRVEEI
                     AAMVAFVAGPEASYITGANLTVDGGMNA"
     gene            complement(1833..2615)
                     /locus_tag="VHV99_14115"
     CDS             complement(1833..2615)
                     /locus_tag="VHV99_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3636594.1"
                     /translation="MTASIYDNIHSTKHAGKVALVTGGSTGIGLATAMRLAREGAKVF
                     VTGRRQAELDAAVSQIAHGALAIRGDISSVADLERIVQTVTAAHPTIDILFANAGGGE
                     FTSLGDISEAQFDKYFGINVKGTLLTVQSALPFMRAGSAIVITGSIASIQGVPAFGVY
                     AATKAALRSFARTWASDLKGRDIRVNVVAPGVVVTPAYKTELKMSDEQIAAYCKEVAG
                     TTPLGRVGTADEIAKAVSFLASDDASYITGIEMFVDGGRAQV"
BASE COUNT          558 a          788 c          827 g          519 t
ORIGIN      
        1 gcgcctcgcg cggcatcggc cgcgccagtg cgctcgcact cgcgaaagcg ggcgcacagg
       61 tgctcgttca ctacagcagc ggggaaaagg ctgcggatgg tgtcgtcgcc gaaatccgcg
      121 cggccggcgg caacgcccag ataatcgccg cggatctgcg cgacgcagac ggtccgcata
      181 cactcgcaaa acgcgtacgc gctgtcgtcg gccaccgtct ggacattctg gtggcgaacg
      241 ccggcatttc aaaggccgca agcatcgagg aaacgaccgt cggagatttc gacgagctgt
      301 tcgccgtgaa tgtgcgtgcg ccttatttcc tcgtgcagca gttactgccc gtgatgtgca
      361 aaggcagcag cgttgtcctg ctctcgtcgc tcgcggcgca tgccgcggtc ggcaagctgc
      421 ccgcatacgc ggctaccaaa ggggcggtcg atacactcgt caagcatttc gcttcggcac
      481 ttggtgaacg cgagattcgc gtgaatgcag tcgcgcccgg cgtcgtcgaa actgacatgt
      541 cgaacttcac caggaccgac gcgggacgcg agctcgcgct cggcatgcag gcgttaaagc
      601 gcgtcgcgca acctgatgac atcggcgaag cggttgcgtt tctcgcctcg gacgcagcgc
      661 gctggattac cggtgaaaca ctgcgtgtcg acggcggctc gaaactctag agcaacccta
      721 ctcacgcccg tttccaaggt cttcgagaag cctgattaat caggcatccg tatctattct
      781 aaaaatcagg gaggacaatt caaataacag ccgccgcacc catacggtac gaatcgaacg
      841 gatctccgaa cgcacgccgg gtgcgcatct tcatcgcgga gaagggaatc gacgtcccga
      901 cgaacgacaa cagtcaatca ttaactggaa accttaggaa atcacatgtc taagttagct
      961 caaaaagtgg cgctcattac cggcggttca cgcggcatcg gtgcggcaat cgcgaagcgt
     1021 ctcgcagcgg atggagcgcg ggtggccttt acctatgcga aggacgccag cgcggcttcg
     1081 gccgtagtca aagagattga actcaatggc ggaaaggctc tcgcgatcca ggcggacgct
     1141 actgacgtca aagctgtcga ggctgcggtc gaaaaaactt ttgcgacctt cggccggctc
     1201 gatgtgctgg tgaacaatgc cggcaccgcc attccgaaac cgttcgaaga cacgacactg
     1261 gaggagatgg atcgagtgat cgacatcaac atccgcggcg tgttcgctac gaccaaggct
     1321 gcgctgaagc acctgggtga tggtggccgc atcatcatga tcggttcggc ggtgggcgag
     1381 cgagccattg ctcccgggct ggtgccctac gcagccacga aagcagccgt caggatgttc
     1441 acccaggcgc tgtccagaga gttgggaggc cggggcatca cggtcaacaa cgtccagccg
     1501 ggaccgatcg ataccgacct gaatcccgcc tcaggcgatt gggcggtgcc gcagaaggcc
     1561 gctacagtac ttggtcgcta tgggcgcgtt gaagagatcg ccgcaatggt agctttcgtc
     1621 gccggcccgg aggcctcgta cattaccgga gcgaatctta ccgtggatgg cgggatgaat
     1681 gcctgagcct gagcacatat tcgacctacg gctttgaagc agagcgacgc atcttttcaa
     1741 ggaactgctg ggcaagacga agattttccc gcgaacccat ggtgttttcc cgtttatgac
     1801 gcgccacgtt cggatgagcg cggcgttgaa aatcagacct gcgcgcgtcc gccgtcgacg
     1861 aacatctcga taccggtgat atagctggcg tcgtctgacg cgaggaagga aacggccttc
     1921 gcaatctcgt cggcggtgcc gacccgacca agcggggttg tcccggcaac ttccttgcag
     1981 tacgccgcga tctgttcgtc gctcatcttc agttcggtct tgtaagccgg cgtcacgacg
     2041 actccgggcg ctacaacgtt gacgcggatg tcgcggcctt tgagatcgga tgcccaggtg
     2101 cgtgcaaacg aacgcaaggc tgccttggtg gcggcataga caccgaatgc aggcacgcct
     2161 tgaatcgatg cgatcgatcc tgtgatcacg attgcgctgc ccgcgcgcat gaatggcaaa
     2221 gcgctttgca cggtgagcag tgtgcctttc acattgatgc cgaagtattt gtcgaattgc
     2281 gcttcgctga tgtccccgag actcgtgaat tcgccgccgc cggcgttggc gaacaggatg
     2341 tcgattgtcg ggtgagccgc cgttaccgtt tgcacgatgc gctcgaggtc ggccacagaa
     2401 gagatgtcgc cgcgaatcgc gagcgcaccg tgggcgattt gcgataccgc ggcatccagc
     2461 tccgcctggc gccggccggt gacgaacacc ttggcgcctt cgcgagcgag gcgcattgcg
     2521 gtggcgaggc cgatcccggt actaccgccg gtcacgagcg ctacctttcc agcatgcttt
     2581 gtcgaatgga tgttgtcgta aatagaggct gtcatctcaa ggctccggtt catctgcaag
     2641 ggtaatggcc tcatactaga ttaagcacat gtacagataa atatgtacat cg
//
LOCUS       DATASH010000511         5225 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4632127,
            whole genome shotgun sequence.
ACCESSION   DATASH010000511 DATASH010000000
VERSION     DATASH010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5225
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4632127"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VHV99_14120"
     CDS             <1..500
                     /locus_tag="VHV99_14120"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000428 - DNA-directed RNA polymerase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEX3636595.1"
                     /translation="AKIIGRIVLDASFSPVRRVSYAVESARVEQRTDLDKLVMNIETN
                     GVISPEEAIRQSARILVDQLSVFAALEGTEATAEAPSRAPQIDPILLRPVDDLELTVR
                     SANCLKAENIYYIGDLIQRTENELLKTPNLGRKSLNEIKEVLASRGLTLGMKLENWPP
                     AGLDK"
     gene            671..1063
                     /gene="rplQ"
                     /locus_tag="VHV99_14125"
     CDS             671..1063
                     /gene="rplQ"
                     /locus_tag="VHV99_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEX3636596.1"
                     /translation="MRHRHGLRKLNRTSSHRRAMLRNMSNSLIEHEVIKTTLPKAKEL
                     RKVVEPLITLGKKPSLANRRLAFNRLRDRDSVTKLFDVLGPRYATRPGGYLRVLKFGF
                     RVGDNAPMALVELLDRPEVEEVEVQEAE"
     gene            1286..1612
                     /gene="cutA"
                     /locus_tag="VHV99_14130"
     CDS             1286..1612
                     /gene="cutA"
                     /locus_tag="VHV99_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004199838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HEX3636597.1"
                     /translation="MNVILILTTVPDATVAQKLAADALAARLCACVTLLGTVQSSYHW
                     QGTIETAQETQLLFKTSCARALELERYIQAHHPYDTPEILSWQATASAAYGQWITAET
                     QRPLHV"
     gene            1605..3479
                     /gene="dsbD"
                     /locus_tag="VHV99_14135"
     CDS             1605..3479
                     /gene="dsbD"
                     /locus_tag="VHV99_14135"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HEX3636598.1"
                     /translation="MFNGPNRRARNALRTVLFLLGCLIFSPFGTLAHAADDFLDPAVA
                     FKFSATEKPGEVDVTYKIANGYYMYRERFAFAVRNGVTTIGEPQLPAGHVKFDQTFNK
                     NVETYRNELTIRIPVKQAQGPFDLAVTSQGCADAGICYPPMERVYHVSGEALQPAGSA
                     AATAAARQPAAAGTSWYERATSADYAQSLLQGGGFFAIIGLYFVAGAVLSLLPCSYPM
                     IPILSAIIIGEGARVTRARGFALSLAYVIGMALVYTALGVAAALVGQSLGAWLQNPWV
                     LGTFGVLLTVFALTLIAGFDIALPQRWQDGVSRASTDRSGGKFAAVGVMGALSALVVG
                     ACMTAPLFAVLAFIAHTGDALLGGAALFSMGLGLGVPLLIIGLGAGSLLPRAGAWMNG
                     VKVFFGVVLLAAALWIVWPVLGATATMLLSALWLLTAAAGLGLFAGPTAGASVWRRLG
                     RGIGAALAIWAAVLLVGLAAGSSDPLRPLAVLAARGGSEATVVSTSNEPDAASQNDLT
                     FASVRSSTELDEAVKTAAQPAMLDFYADWCVSCKEMEKFTFSDPRVQAKLKQMNLLRA
                     DVTANNADDQMLLKRFSLFGPPGIIFFDQGGKEVLRVVGYESADKFLRSLDRASAPGA
                     "
     gene            complement(3600..4598)
                     /gene="hemB"
                     /locus_tag="VHV99_14140"
     CDS             complement(3600..4598)
                     /gene="hemB"
                     /locus_tag="VHV99_14140"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009913842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX3636599.1"
                     /translation="MSFYPHYRPRRMRRDDFSRRLMRENILTTNDLIYPVFIVEGANV
                     RQAVPSMPGVERVSVDLLMGVAEQCVELGVPVLSLFPAIEPSLKTPDGREATNPAGLI
                     PRAVRELKKNFPDLGVLTDVALDPYTSHGQDGVLDEAGYVINDETVEILVEQARAQAE
                     AGVDIVAPSDMMDGRIGAIREMLESEDYIHTRIMAYAAKFASAFYGPFRDAVGSATNL
                     GKGNKMTYQMDPANSNEALREVRADIEEGADMVMVKPGMPYLDIVRRVKDEFKFPTYV
                     YQVSGEYAMLKAAAQNGWLDHDKAMMESLLAFKRAGADGVLTYFALDAARILRSQK"
     gene            complement(4859..>5225)
                     /locus_tag="VHV99_14145"
     CDS             complement(4859..>5225)
                     /locus_tag="VHV99_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEX3636600.1"
                     /translation="HWEALLSTYLQSRSQLRGMILMMDSRRPLTDLDRRMIEWFAPTG
                     KPIHTLLTKCDKLTRQESVNALRATQKGLAEYRAAGYQGELTAQLFSALKRVGIDEAH
                     ALIESWLIPDAKGETDAPQ"
BASE COUNT          954 a         1601 c         1595 g         1075 t
ORIGIN      
        1 cggccaagat catcggtcgt atcgtgctgg atgcgtcgtt ctcgccggtt cgccgcgtga
       61 gctacgccgt tgaaagcgct cgcgtcgaac agcgtaccga cctcgacaag ctcgtgatga
      121 acatcgaaac caacggtgtg atctcgccgg aagaagcgat ccgtcaatcg gcgcgtattc
      181 tggttgatca actgtcggtg ttcgctgcac tggaaggtac cgaagcaacg gctgaagcgc
      241 cgtcgcgtgc gccgcagatc gatccgatcc tgctgcgtcc ggtcgatgat ctcgaactga
      301 cggttcgctc cgcgaactgc ctgaaggccg agaacatcta ctacatcggc gacctgatcc
      361 agcgcaccga aaacgagctg ctcaagacgc cgaatctggg ccgcaagtcg ctgaacgaaa
      421 tcaaggaagt actggcgtcg cgtggtttga cgctcggcat gaaactcgaa aactggccgc
      481 cggcagggtt ggacaagtaa gtcgacaagt agtctcggga cgagccacgc cgctgcactg
      541 gcaaagacgc ggattttcct ttaaaatccg cgtcttgtct tttttaacta ccggcccgtg
      601 ccctcatatc cttcgggtaa ttgagcgcga tagaagagct ggaccaaaac tttgaattga
      661 aggaattaac atgcgtcacc gtcatggtct gcggaaactg aaccgcacga gcagccaccg
      721 tcgggcaatg ctccgtaata tgtccaactc gctgatcgag cacgaagtca tcaagacgac
      781 gctgccgaag gcgaaagaac tccgtaaagt cgtcgagccg ttgatcacgc tcggcaagaa
      841 gccgtcgctg gcaaatcgtc gtctggcgtt caaccgcctg cgcgatcgtg attcggtcac
      901 gaagctgttc gacgtcctcg gcccgcgtta cgctacccgt ccgggcggct acctgcgcgt
      961 cctgaagttc ggcttccgcg tcggcgacaa cgcaccgatg gcactggtcg aattgctgga
     1021 ccgtccggaa gttgaagaag ttgaagtgca agaagctgaa taagctttta gcattgaaaa
     1081 aaagccaggc aaccagcctg gcttttttgt ttgcggcggt gaaattcact gctgcatccc
     1141 gatcgcggcg tccaactgcc gcatgctcgc cgctgaggca cctgaggcac ctgaggcacc
     1201 tgagacacca gcggcacccg ccgcaccgca aggccaggtg ataatatcag cacctgaatc
     1261 gtgcctgccc ggagcctttg tgagcatgaa tgtaatcttg attctgacga cggtaccgga
     1321 tgccaccgtt gctcagaagc tcgccgcgga tgcactggct gcgcggctat gtgcctgcgt
     1381 cacgctgctg ggcacggtgc agtccagcta tcactggcag ggtacgatcg aaacggcgca
     1441 ggagactcag ttactgttca agacgagctg cgcacgcgcg ctggaactcg agcggtacat
     1501 tcaggcgcac catccctacg acacgccgga aatcctctcg tggcaagcca cggcatcggc
     1561 cgcgtacggc cagtggatca ctgctgaaac ccaacgtcct cttcatgttt aacggtccaa
     1621 atcgacgtgc acgcaatgcg ctacgcacgg tccttttcct gctcggctgc ctgatcttct
     1681 cgccgttcgg cacgcttgcc cacgccgcgg acgacttcct cgatcccgcc gtcgcgttca
     1741 aattcagcgc gaccgaaaag cccggtgaag tcgacgtcac ttacaagatc gcgaacggtt
     1801 actacatgta ccgcgagcgc ttcgcattcg cggtccgtaa cggcgtgaca acgatcggcg
     1861 agccgcaatt gcccgctggc cacgtgaagt tcgatcagac cttcaacaag aatgtcgaaa
     1921 cctatcgcaa tgaattgacg atccgcattc ctgtgaaaca ggcccagggg ccgttcgatc
     1981 tcgctgtcac gtctcaaggc tgtgccgatg ccggaatctg ctatccgccg atggagcgcg
     2041 tgtaccacgt cagcggcgag gcgttgcagc ccgccggcag tgcagccgct actgccgccg
     2101 cacggcaacc cgcagcagcc ggcacctcat ggtacgaacg cgccaccagc gccgactacg
     2161 cgcagtccct tctgcaaggc ggcggcttct tcgcgatcat tgggctctat ttcgttgctg
     2221 gcgccgtgct cagtttgttg ccgtgctcgt atccgatgat cccgatccta tcggcgatca
     2281 tcattggcga aggcgcgcgg gtgacgcgcg cccgcggctt cgcgttgtcg ctggcgtacg
     2341 tgatcggcat ggcgcttgtg tacacggcgc ttggcgtcgc ggcggcgctg gttggccaga
     2401 gcctcggcgc gtggctgcag aacccatggg tgcttggaac gtttggcgta ttgctgacag
     2461 ttttcgcatt gaccctgatc gcgggtttcg acatcgcgtt gccacagcgc tggcaagacg
     2521 gtgtgtcgcg agcgtcgacc gaccgttccg gcggcaagtt tgccgcggtt ggcgtgatgg
     2581 gcgcgctgtc cgcgctagtg gtgggcgcct gcatgacggc gccactcttt gccgtgctgg
     2641 cattcatcgc gcataccggc gatgcattgc tcggcggcgc ggcgctgttc tcgatgggac
     2701 tcggtctcgg tgtgccgctc ctgatcatcg gtcttggcgc cggtagtttg ttgccgcgcg
     2761 ccggcgcatg gatgaacggc gtcaaggtgt ttttcggcgt ggtgctgctt gcagcggccc
     2821 tgtggatcgt ctggccggtg ctcggtgcga cagcaacaat gctgttgagc gcgctgtggc
     2881 tgctgaccgc cgccgccgga ctcggtcttt tcgcgggccc aaccgcaggc gcctcggtgt
     2941 ggcgccggct cggccgtggt atcggcgcgg cactggcaat ctgggcggcg gtgctgctgg
     3001 tcggcctggc tgcgggctcg tctgatccgc tgcgtccgct ggctgttctg gccgcgcgcg
     3061 gcggtagtga ggctactgtc gtaagcacct ccaatgagcc tgatgcagct tcccagaacg
     3121 atctgacgtt cgcctcggtg cgttcctcga ccgagctcga cgaggccgtc aaaacggctg
     3181 cgcaaccggc tatgctcgat ttttacgccg actggtgcgt gagctgcaag gagatggaga
     3241 aattcacgtt cagcgatccg cgtgtccagg cgaagttgaa gcagatgaac ctcctgcgcg
     3301 ccgacgtcac tgcgaataac gctgatgatc aaatgctgct caagcgtttc agcttgttcg
     3361 ggccgcctgg catcatcttc ttcgatcaag gcggcaagga agtattgcgc gtcgtcggat
     3421 acgaatccgc agacaagttc ctgcgcagcc tcgatcgcgc gagtgcgccc ggcgcctagc
     3481 ggtggtgcgg ttttccgcaa gggtgtgcaa aaaaaggagg cgtgactgaa aaagtccgcc
     3541 tccgtttctt ttgagccatc agaccgccgc cgaccgcctc ggcgaagcgt cgaatcaaat
     3601 cacttctgcg agcgcaaaat tcgcgcggca tccagcgcga agtaggtcag cacgccatcg
     3661 gcgcctgccc gcttgaacgc cagcagcgat tccatcattg ccttgtcgtg gtccagccag
     3721 ccgttctgcg cggccgcctt caacatcgcg tattcgccgc tcacctggta cacgtaagtc
     3781 gggaatttga actcgtcctt cacgcgacgc acgatgtcca gatacggcat gcctggcttg
     3841 accatcacca tgtccgcgcc ttcctcgatg tccgctcgca cttcgcgcag tgcttcattc
     3901 gaattggccg ggtccatctg gtaggtcatc ttgttgccct tgccgaggtt cgtggcggaa
     3961 ccgacggcat cgcggaacgg gccgtaaaac gccgacgcga acttggccgc gtaggccatg
     4021 atccgcgtgt ggatatagtc ctcgctctcg agcatttcgc ggatcgcgcc gattcggccg
     4081 tccatcatgt ccgagggcgc gacgatatcg acccctgctt cagcctgcgc acgcgcctgt
     4141 tcaacgagaa tctcgacggt ctcgtcgttg atcacgtagc cggcctcatc gagtacgccg
     4201 tcctggccgt ggctcgtgta cggatccagc gcgacgtcgg tgagcacgcc gaggtcgggg
     4261 aaattctttt tcagctcgcg aaccgcgcgc gggatcaaac cggcgggatt ggtcgcttcg
     4321 cggccatcag gcgtcttcaa ggaaggctcg atcgcgggga acagcgacag cacgggtacg
     4381 cccaactcaa cgcattgttc ggccacaccc atcagcaggt cgaccgacac acgctcgacg
     4441 ccgggcatcg acggtacggc ttgccgcacg ttggcgcctt cgacgatgaa cacggggtag
     4501 atcagatcgt tggtggtgag gatgttttcc cgcatcagac ggcgcgagaa gtcgtcgcgg
     4561 cgcatgcggc gcggacgata gtgcgggtag aagctcataa acgcatcgaa ggaacgtgga
     4621 tggatggagt ggacaagtaa cgcctgcctc cgctcccgga aacttttcga gacaatggta
     4681 tatcataccg atcgagcaag gcgcctagcg ctgacctccc cgcttctcct ccctgagcgg
     4741 gtgcctgtcg ttttttcgat taggctgttt gacccgccgt taaagcggcg ggttttttta
     4801 tgggcggccg aaatcggcgc aaaaggcgcc cttgcccgcg ctcccacgag ccgtgcggtt
     4861 actgtggggc gtctgtttcg cccttcgcat cggggatcag ccagctttcg atcagcgcgt
     4921 gcgcttcgtc gatcccgacg cgtttgagcg ccgagaacag ttgcgcggtc agttcgcctt
     4981 ggtagccggc ggctcgatat tccgccagac ctttctgcgt cgcgcgcaac gcgttcacgc
     5041 tttcctggcg cgtcaatttg tcgcatttgg tcagcagcgt gtggatcggc ttgccggtcg
     5101 gcgcgaacca ctcgatcatg cggcggtcca gatcggtcag cgggcggcgc gagtccatca
     5161 tcaggatcat gccgcgcaat tgcgagcgcg attgcagata cgtggacagc agcgcttccc
     5221 agtga
//
LOCUS       DATASH010000512         3371 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5424410,
            whole genome shotgun sequence.
ACCESSION   DATASH010000512 DATASH010000000
VERSION     DATASH010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5424410"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            287..1345
                     /gene="queA"
                     /locus_tag="VHV99_14150"
     CDS             287..1345
                     /gene="queA"
                     /locus_tag="VHV99_14150"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEX3636601.1"
                     /translation="MLTLSDFDFDLPPELIAQVALPERSASRFLEVANTADATDTARL
                     IDRRFAELPECIETGDLLVFNDTKVLKARFLGQKASGGKIEVLVERLIGERTALAQIR
                     ASKSPQPGTAIRLADAFDVTVGERVEPFFTLHFPDDCLTLIEQFGRLPLPPYIEHDPD
                     AIDETRYQTVFAQNPGAVAAPTAGLHFDDALLARLDAPGVERATLTLHVGAGTFQPVR
                     VENLAEHKMHSEWYHLPQALADKIAATRARGNRVIAVGTTSMRALEAAARDAEAAGRP
                     LGAASTETDIFITPGYQFRVVDRLVTNFHLPKSTLLMLVSAFAGVGTIREAYRHAIEQ
                     RYRFFSYGDAMLLTRRDA"
     gene            1597..2814
                     /gene="tgt"
                     /locus_tag="VHV99_14155"
     CDS             1597..2814
                     /gene="tgt"
                     /locus_tag="VHV99_14155"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006416749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEX3636602.1"
                     /translation="MTEGHTHDISGTCNTCASHDKGAPSRPDNGLKFELLGTDGQARR
                     GRVTLNHGVVETPIFMPVGTYGTVKAVQPRELEEMHAQIILGNTFHLWLRPGLETIEA
                     HGGLHGFMGWNKPILTDSGGFQVFSLGDLRKITEDGVTFASPINGDKLFLSPEVSMQI
                     QKVLNSDIVMQFDECTPYATNNVPTSHQEAADSMRMSMRWARRSIDEFNRLGNPNALF
                     GIVQGGMFEDLRDESLAGLAEMDFHGLAIGGLSVGEPKEDMMRVLNHIGPKLPADKPH
                     YLMGVGTPEDLVAGVAAGVDMFDCVMPTRNARNGWLFTRFGDIKIRNAAHKNSLRPLD
                     EQCGCYTCRNFTRGYLHHLHRVGEILVAQLNTIHNLHYYLELMQEMRDAIDAKLFEPF
                     RKRFHENRARGID"
     gene            3021..3350
                     /gene="yajC"
                     /locus_tag="VHV99_14160"
     CDS             3021..3350
                     /gene="yajC"
                     /locus_tag="VHV99_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEX3636603.1"
                     /translation="MSFISNAFAQGTATGGIESNLMSFLPLILMFGVLYFIMIRPQMK
                     RQKEHRNMLAAMAKGDEVVTNGGIVGKVTKVGEAYVGVEISEGTEITVQKASVTTILP
                     KGTIKSL"
BASE COUNT          645 a         1101 c         1004 g          621 t
ORIGIN      
        1 cttcgcgttc tccaccgtga cactggcgtc gccctcgctt tcctgcgccg caaccgcctt
       61 gccccgcgcg acgcggcgct tcggttcggc gctcacttca tcggtagagg cgggcaaacg
      121 gcggtcggac aaaggcatgg gctgcttcgc aagtacaata tcggctgtca aagggttcgg
      181 cgccatacag cgcgcgggct cgcgggccgc tctggcgtcc cgcaatcata gccgcccggc
      241 tccggcttcg gctcaattca gtcccaattc gcttccagtc gtccgcatgc ttacgctttc
      301 cgatttcgat ttcgatctgc cacccgaact gatcgcgcaa gtcgcgctgc ccgagcgcag
      361 cgccagccgt ttcctcgaag tggccaacac agctgatgcc accgacaccg cgcgcctgat
      421 cgaccgccgc tttgctgagc tgcctgagtg catcgagacc ggcgatctgc tggtcttcaa
      481 cgataccaaa gtcctgaagg cgcgcttcct cggccagaag gccagtggcg gcaagatcga
      541 agtgctggta gagcgtctga tcggcgaacg caccgcgctc gcgcagatcc gcgcaagcaa
      601 gagcccgcag ccgggcaccg cgatccgtct cgccgacgca ttcgacgtga cggtcggcga
      661 acgcgtcgag ccgttcttca cgctgcattt ccccgacgac tgcctgacgc tgatcgaaca
      721 attcggccgc ctgccgctgc cgccatacat cgagcacgat cccgacgcga tcgacgaaac
      781 ccgctaccag accgtgttcg cgcagaaccc gggcgcggtg gccgcgccca ccgccggcct
      841 gcacttcgac gacgccctgc tcgcgcggct cgacgccccc ggcgtagagc gcgcgacgct
      901 gacgctgcac gtcggcgcgg gcacgttcca gccggtgcgg gtcgaaaatc tcgccgagca
      961 caagatgcac agcgagtggt atcacctgcc acaagcgctc gccgacaaga tcgcggcgac
     1021 gcgcgcgcgc ggcaaccgcg tgatcgcggt gggcacgacg tcgatgcgcg cgctcgaagc
     1081 cgccgcgcgc gatgccgaag ccgccggacg cccgctcggt gcggccagca cggaaaccga
     1141 cattttcatc acgccgggct atcaattccg cgtggtcgac cggctggtga ccaatttcca
     1201 tttgccgaag tcgacgttgc tgatgctggt gtcggcgttc gcgggtgtcg ggacgattcg
     1261 cgaagcgtac cggcatgcga tcgaacagcg ttaccggttc ttcagttatg gcgacgcgat
     1321 gctgctgacg cggcgcgatg cctgatacgt tccgcgtcgt gcggcgaggt tccgtgcgat
     1381 agcgttgcgc gcaggcggcg cgcacggtag tctgccgctc cttgctgctt tgatgcagcg
     1441 gcggtcgttg ccgcaccgct tgtgcgtgct tgtacccgcg tgcgcagcgg cagacttgcg
     1501 ctgcatcttg agttttcctt acccggccgc cgcggcttac ccggcggcgt tgatcatttg
     1561 cgccggactg ttttccggtg gcacaggagt cccccaatga ccgaaggtca tacccacgat
     1621 atcagcggta cctgcaacac ttgcgcgagc cacgacaagg gcgccccttc gcgccctgac
     1681 aacggcctca aattcgaact gctcggcacc gacggccagg cccggcgcgg tcgcgtcacg
     1741 ctcaaccacg gcgtggtcga aacacccatc ttcatgcccg tcggcacgta cggcacggtg
     1801 aaagcggttc agccgcgcga gctggaagaa atgcacgcgc agatcatcct cggcaacacc
     1861 tttcacttgt ggctgcgccc gggtctggaa acgatcgaag cgcatggcgg cctgcacggc
     1921 ttcatgggct ggaacaagcc gatcctgacg gattccggtg gctttcaggt cttttcgctt
     1981 ggcgatctgc gcaagataac cgaagatggc gtcacattcg cgtcgcccat caacggcgac
     2041 aaactcttcc tgtcgccgga agtgtcgatg cagatccaga aggtcctgaa ctcggacatc
     2101 gtcatgcagt tcgacgaatg cacgccgtac gcaaccaaca acgtgccgac ttcgcatcag
     2161 gaagccgctg attcgatgcg catgtcgatg cgctgggcca ggcgctcgat cgacgaattc
     2221 aaccgcctcg gcaatccgaa tgccctcttc ggcatcgtcc agggcggcat gttcgaagac
     2281 ctgcgcgacg agtcgctcgc gggcctcgcg gagatggatt tccacggtct cgcgatcggc
     2341 ggcctctcgg tcggcgagcc gaaagaagac atgatgcgcg tgctgaacca catcggcccg
     2401 aagctcccag ccgacaagcc gcactacctg atgggcgtcg gcacgccgga agacctggtg
     2461 gcgggcgtgg ccgccggcgt cgacatgttc gactgcgtga tgccgacccg caacgcgcgc
     2521 aacggctggc tcttcacgcg tttcggcgac atcaagatcc gcaacgccgc gcacaagaac
     2581 tcgctgcgcc cgctcgacga gcaatgcggt tgctacacct gccgaaattt cactcgcggc
     2641 tacctacatc acctgcatcg tgtaggagaa atcctcgtgg ctcagttgaa cacgattcac
     2701 aaccttcact actacctcga attgatgcag gaaatgcgcg acgcgatcga cgcaaaacta
     2761 ttcgagcctt tccgcaagcg ttttcacgag aatcgcgcgc gcggcataga ctagtcggcg
     2821 atcggcaatc agccgacgaa tttgccggag gaatccatcg gcttcacgcg gaaaacctta
     2881 caataaactt tcgcgaacgg cgaaaaaggc tttaaacggt tgatcgcaaa gccgattcgc
     2941 cgcgctgacc cctgtctggc cggtggtaga ataaccggct gatttttttg atcgttttga
     3001 cttataacgg agagaccaac gtgtcgttca tttccaatgc cttcgcccaa ggcacagcaa
     3061 caggtggcat tgaatcgaac ctgatgagct tcctgccgct gatcctgatg ttcggcgtgc
     3121 tgtacttcat catgattcgc ccgcaaatga agcgccagaa ggaacatcgc aacatgctcg
     3181 ccgccatggc caagggcgat gaagtggtga cgaatggcgg catcgtcggt aaggtgacca
     3241 aggtgggtga agcttacgtc ggcgtcgaaa tctcggaagg cacggaaatc accgtgcaaa
     3301 aagcgtcggt cacgacgatt ctcccgaagg gcacgatcaa gtcgctgtaa ggcctgctct
     3361 ctcgcgtccg g
//
LOCUS       DATASH010000513         8920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_81465,
            whole genome shotgun sequence.
ACCESSION   DATASH010000513 DATASH010000000
VERSION     DATASH010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 8920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8920
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81465"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..266)
                     /locus_tag="VHV99_14165"
                     /pseudo
     CDS             complement(12..266)
                     /locus_tag="VHV99_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182694.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            complement(339..1652)
                     /locus_tag="VHV99_14170"
     CDS             complement(339..1652)
                     /locus_tag="VHV99_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX3636604.1"
                     /translation="MNRDPAPHHPLVQRLIDARQTTDALFSIVKPEFLYERPIRERHR
                     IVFYVGHLEAFDRNLFDQRLCELPAFDPHLDQLFAFGIDPVDGGFPTDQPSDWPSLDA
                     VRDYALRAREQIDRELDALSDAARVGRENAQAQASGQLLNVAIEHRLMHAETLAYMLH
                     QLPLAQKVTELREPVLTRSEPRAALSSMVSVPAGATVLGMSRESGHFGWDNEFGEMRV
                     DVPAFEIDRHMVTNGAFREFIEAGGYREPKWWTEKDWAWKEAEHIEHPACWSRHTGSA
                     QNSAWTLRTMFDEIPLPLDWPAYVSHAEASAYARWAGKALPSEAQWQRAAHGAPHAMS
                     GNFDFRSWDPLPVDAHPDNVSAFGVEGQFGNGWEWTSTVFDALPGFEAFPFYPGYSAN
                     FFDGQHYVIKGGSPRTAQCMLRPAFRNWFQPHYQYVYAGFRCVKA"
     gene            complement(1784..2527)
                     /gene="fnr"
                     /locus_tag="VHV99_14175"
     CDS             complement(1784..2527)
                     /gene="fnr"
                     /locus_tag="VHV99_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HEX3636605.1"
                     /translation="MISPPARGGRRAAPVRATARTAARCSSCAMRALCMPQGLSPDEL
                     TKLEALICTARSVQRGEALYRSGDRFDSIYAVRSGSMKTVMAHRDGREQVTGLRLAGE
                     ALGLDGISEDMHACSAVALEDSTVCIVPYPALKSLCREISSMQDRLHRLLGEQIVREA
                     GQMMVLGSLSADERVAAFLLDVSERNAQRGYSSAEFNLRMTREDMGSYLGMTLETVSR
                     TLSRFQKRGLIDTQGKHIRIVDLEGLQKV"
     gene            complement(2820..3287)
                     /locus_tag="VHV99_14180"
     CDS             complement(2820..3287)
                     /locus_tag="VHV99_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX3636606.1"
                     /translation="MYTRIIVAVDGSHTSRRAFEAALALAKSTGAVMQPFYVVENTPM
                     YFEAPGYDPSVLRNRLIEEGKELGAEFAQAMAAQGVKGELAVGEASSLDDVSVVVLKA
                     AAEFNADLLVMGTHGRRGFQRLILGSVAERCVRQASLPVLLIPSAAGKESEAI"
     gene            3580..4269
                     /locus_tag="VHV99_14185"
     CDS             3580..4269
                     /locus_tag="VHV99_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEX3636607.1"
                     /translation="MNERKTTGLPDKIYSDILNRILEGEYKQGERLPTEHALAERFAT
                     SRPTVREALARLRADGIIMTRHGSGTTVARRPDPDVRRFAPLETLSDIRRCYEFRIVT
                     EAGAAEQAALKADADDIAAIQHAWDQLERIIETHGIGVQDDFIFHLAVARASKNPFFI
                     TVMSFIEEQILFSMNLSRNLSLVKTLERRRLVQAEHLAVLEAIRRKDGPAAGRAMRAH
                     LENALERMFGS"
     gene            complement(4546..5139)
                     /locus_tag="VHV99_14190"
     CDS             complement(4546..5139)
                     /locus_tag="VHV99_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-homoserine-lactone synthase"
                     /protein_id="HEX3636608.1"
                     /translation="MQTAIRIGMRQEFDNADINEMYRLRARVFHGRLGWDIPTIAGME
                     IDGYDALGPHYMLIQGDDGQVRGCWRLMPTEGPNMLKDTFPQLLHGADAPVGRHIWEL
                     SRFAIETSGEEQSFGFADLTMQAIHELVTFADRMGITRYVTVTTTPIERLLRKTGIDI
                     SRLGSPLQIGVERAVALDIAVSPKTRTALFGPMAAAA"
     gene            5293..5610
                     /locus_tag="VHV99_14195"
     CDS             5293..5610
                     /locus_tag="VHV99_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEX3636609.1"
                     /translation="MELLENHWQAQPSVQTRPVEPSSAVDRVLIIAYRKKKKESQRRF
                     WARFGVTQSRGSRFESGAEIPAPVSILLGLYFTKTVSDADLGRAERVLYSRDAAALLN
                     PGQ"
     gene            complement(5594..6298)
                     /locus_tag="VHV99_14200"
     CDS             complement(5594..6298)
                     /locus_tag="VHV99_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HEX3636610.1"
                     /translation="MAPLLNAADEAEWFSAIAGLADTWGFDRLLIAMLPRPTIRLEDA
                     YVRSTYSPTWRRTYDEQGLVHIDPTVAHCATRAAPLIWSPDIFTTAPQQSMYEEARAH
                     GLRAGVTLPIHGPNQEAGMMCFVNDSNPTDAFWRHVNVALPNLVLLRDLVIDTSQRHL
                     NTHAQTLLPKLTPRERECLKWTARGKSTWEISHILSCSEAVVNFHMKNIRTKFGVNSR
                     RAAAVIAAQLGLIDPG"
     gene            6911..7063
                     /locus_tag="VHV99_14205"
                     /pseudo
     CDS             6911..7063
                     /locus_tag="VHV99_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092372.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
     gene            7206..8150
                     /locus_tag="VHV99_14210"
     CDS             7206..8150
                     /locus_tag="VHV99_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX3636611.1"
                     /translation="MTDNNRRYPDPSIRVLDPRFKSLILASASVECLYQGARWSEGPV
                     WFGDGRYLLWSDIPNDRILRWDETSGAVSTFRQSSNNANGHTRDRQGRLVSCEHLTRR
                     VTRTEYDGSITVLADRYRGKRFNSPNDVVVKSDGSIWFSDPTFGIDGFYEGERQESEL
                     PACVYRIDGQSGEVTVVADDILGPNGLAFSPDESVLYIVESRGAPRKIRAFDVDAGGD
                     SAALSNNRVLIDAGPGTPDGFRVDVHGNLWCGWGMGTDELDGVRVFTPQGEPIGHIAL
                     PERCANVCFGGRHRNRLFMAASHGLYSLNVNTQGVQGG"
     gene            8438..>8920
                     /locus_tag="VHV99_14215"
     CDS             8438..>8920
                     /locus_tag="VHV99_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3636612.1"
                     /translation="MTVSGRLALRLVSVCLVASAACASAAHAADPVTLNIVDVAGDLQ
                     LTQKGFEAFKAKYPNLVDNLTFTNAPAPQLPGKIKAMQGAGRSDIDLVLTGTDALAAG
                     IEQNLWIKLLPDNAAAFPGVLDKYAPGPRRMQDLAQGFGLEVAYMPAGPLLEYNPAKV
                     S"
BASE COUNT         1760 a         2755 c         2640 g         1765 t
ORIGIN      
        1 gatgaggatg cttacatcga ccaagaatag aactgttcgg cgggagtttg gccgctacca
       61 ctgtctttca cctgcccctt tgcaatcatg tgcatgggct cgataccgcc cagcaaagct
      121 cgggcacaac gaaaagtctt gaacccgagc atcggtcggg tacggcgctt gatagcccgg
      181 tggtcctgtt cgatgatgtt gttcagatac ttgttctggc ggatcctgat cggcgtgtcg
      241 cgctccgcgt tgagggcctc gagcgctgta aaagcaataa ttggttgtta ttgcagttaa
      301 gcgaaagatg attggcgcgt catttttcgc ttaacttgtc acgccttcac acatcgaaat
      361 cccgcataca cgtactgata gtgcggctga aaccagttcc ggaaagccgg ccgcaacatg
      421 cattgcgcgg tgcgcggcga accacctttg atcacgtaat gctgcccgtc gaaaaagttg
      481 gcggagtagc cgggatagaa cggaaacgct tcaaatcccg gcagtgcgtc gaacaccgtc
      541 gaggtccact cccagccatt gccgaactga ccttccacgc cgaacgcgct gacgttgtcg
      601 ggatgcgcgt cgaccggtag cgggtcccag ctgcggaaat cgaaattgcc cgacatcgcg
      661 tgcggtgcgc cgtgcgcggc ccgttgccat tgtgcttcgc tgggcagtgc cttgccggcc
      721 cagcgtgcgt aggcgctggc ttcggcatga ctcacgtagg cgggccagtc gagcggcagc
      781 ggtatttcgt cgaacatcgt gcgcagcgtc catgcgctgt tctgagcgct tccagtgtgc
      841 ctggaccagc acgccggatg ctcgatatgc tcggcttcct tccaggccca atccttctcc
      901 gtccaccact ttggttcgcg atagccacct gcctcgatga actcgcgaaa cgcgccgttc
      961 gtcaccatgt ggcggtcgat ctcgaacgcc ggcacgtcga cgcgcatttc gccgaactcg
     1021 ttgtcccagc caaaatgccc gctctcgcgc gacatgccga gcaccgttgc gccggccggc
     1081 acgctgacca tcgacgatag cgccgcgcgc ggttcagaac gcgtgagcac cggctcgcgc
     1141 aattcggtga ccttctgcgc aagcggcaac tgatgcagca tgtacgcgag cgtttcagca
     1201 tgcatcagcc gatgctcgat cgcgacattc agcaactgcc cggacgcctg agcctgtgcg
     1261 ttctcccggc cgacacgcgc cgcgtcgctc aacgcatcga gctcgcggtc gatctgctcg
     1321 cgggcgcgca gcgcgtagtc acgcacggcg tcgagagacg gccagtcgct gggttgatcg
     1381 gtgggaaagc cgccgtcgac cggatcgatc ccgaaagcga acagctggtc gaggtgcgga
     1441 tcgaacgccg gcagttcgca caggcgttga tcgaaaagat tacgatcgaa agcttccaga
     1501 tggccgacgt aaaacacgat ccggtggcgc tcgcggatcg gccgttcata tagaaactcg
     1561 ggtttgacga tggaaaacag tgcgtccgtg gtttgacgcg cgtcgatcag acgctggacg
     1621 agcgggtgat gcggggcggg gtcgcgattc atttcgccag gtctccttgc gggggacgga
     1681 ctaaagactg tacccgctcg cagaagccat gccaagacga atgcgcgcta aaaagcagtt
     1741 cgacgaagac ctggccgcgc gcgccaggtc ctgcgtgtaa ggcttatacc ttctgcaggc
     1801 cttccagatc gacgatccgg atgtgtttgc cttgtgtgtc gatcaggccg cgcttttgaa
     1861 atcttgacag ggtgcggctg acggtttcga gcgtcatgcc gaggtagctg cccatgtcct
     1921 cgcgcgtcat gcgcaagttg aattccgccg atgaatagcc gcgctgcgcg ttgcgttccg
     1981 acacgtcgag cagaaatgcc gccacgcgtt cgtccgcgga aagcgagccg agcaccatca
     2041 tctgtccagc ttcgcggacg atctgctctc ccagcagtct gtgcaggcgg tcctgcatcg
     2101 aactgatttc acggcacaag gacttgaggg cagggtacgg gacaatacaa acggtgctgt
     2161 cttccagggc gacggcgctg catgcatgca tgtcttcact gataccgtcg aggccgagcg
     2221 cttcgcccgc gaggcgcagg ccggtgacct gttcgcggcc gtcacggtgc gccatgacgg
     2281 ttttcatcga accggaacgc acggcgtaga tactgtcgaa gcggtcgccg ctgcgataca
     2341 gcgcttcgcc acgctgcacc gagcgcgcgg tgcaaatgag cgcttcgagt ttggttagtt
     2401 cgtcgggcga taagccttgt ggcatgcaga gcgcccgcat cgcacaactc gaacagcgcg
     2461 cggcggtgcg cgctgttgct ctcaccgggg cggcacggcg gccgccgcgg gccggtggag
     2521 aaatcagact ggaatcggcg gtaccagagg agcgagtcac agcggtagga gtaagcatgt
     2581 tctgcaacca ttatcagggt aacgatccat tgtcccaccg cccatggccc ccgtttagcg
     2641 acaaaatgac gcgtttggtc agttaggcat cttgagcaat gtcaccctcg tgttgctgtt
     2701 tgtggttatg gactgctatg cattcgggca agcatgttcc gctctgattt tggagatagg
     2761 agcggcgcgt gccgccccat gcggcaagca cgagctttgc aatgtgtgtc accacgcgtt
     2821 cagatggctt cgctttcctt gcctgccgcg gacggaatca acaggacggg caggctcgcc
     2881 tggcgcacgc agcgctcggc gacgctgccg agaatcagtc gctgaaaacc gcgccggcca
     2941 tgcgtgccca tcacgagcag atcggcgttg aactcggcgg cagcttttag cacgaccacc
     3001 gaaacatcgt cgagcgagga ggcctcgccc acggcgagtt cacccttgac gccttgggcc
     3061 gccatcgcct gggcgaattc cgcgcccagt tccttgccct cttcgatcag gcggttacgc
     3121 aaaacggacg ggtcgtaacc gggcgcttcg aaatacatcg gcgtgttttc gaccacgtag
     3181 aacggttgca tgacggcacc ggtggatttc gcgagcgcaa gcgctgcttc gaatgcacgg
     3241 cgcgaggtgt ggctgccgtc gaccgctaca atgatgcgtg tgtacatatc gactccgcgt
     3301 ctaaaggatt cggatgccat ggccgtttgc ccggcccggc tcgttgacga tcgatttatt
     3361 atcgatgcga gcttaaatgt tcgctgaaaa agatgcatcc agtcagaatg taaatcaaat
     3421 acttatcaaa tttcaagatt gcagcgttct acgcggcaaa caccgtgtag cgggcgtttg
     3481 cggtattcct gcgggaccag ggcgcatata tcgatctaga tagcgccagg tttgccttac
     3541 aatcaaaacc tattcagaca agctgctttc gcaccgctga tgaacgaacg taaaacgacg
     3601 ggtttgcccg acaaaatcta cagcgacata ctcaatcgca ttcttgaagg cgagtataag
     3661 caaggcgagc ggctgcccac cgagcatgca ttggccgaac gctttgcaac ctcacggccg
     3721 accgtgcgcg aagcgctcgc gcgcttgcgc gcggacggga tcatcatgac gcggcacggg
     3781 tcgggcacca cggttgcgcg gcggccggac ccggatgtgc gccgctttgc gccgctcgaa
     3841 acgctgtcgg acatccgccg ctgctatgaa tttcgcatcg tgaccgaagc gggcgcggcc
     3901 gagcaggcgg cgctcaaggc cgacgccgac gacatcgccg cgatccagca tgcatgggac
     3961 caactggaac gtatcatcga aacgcatggc atcggcgtac aggacgattt catctttcac
     4021 ctggcggtgg cgcgcgcgtc caagaatccg tttttcatca cggtcatgtc gttcatcgaa
     4081 gaacagattc tgttcagcat gaacctgtcg cgcaatctgt cgctcgtgaa gacgttggag
     4141 cggcggcggc tcgtgcaggc cgaacacctg gccgtgctcg aggcgatccg ccgtaaggac
     4201 ggccccgctg cggggagggc gatgcgcgcg cacctggaga atgcgctcga gcggatgttc
     4261 ggttcctgat attccgcgcg tcgcctcccg cgggccacaa ctgcgggata ggggccagtt
     4321 tgctgccgcc gggcaagctc aacattgcaa cgggcagccg gcagccgcag ccgcacccgc
     4381 accggggcaa tcccaacctt gcaacggcac tccaggcagc gggcaacccc accccgcgtg
     4441 cctgcgggca ggagagccgc accccgggca gcccaccccg cgtatctgcg gccaggcgac
     4501 ccgcaccccg ggcaaccaca cttcggagcc gactcgcccc agacgctaag cggcggcagc
     4561 catcggtccg aacagggctg tgcgggtttt cggactgacc gcgatatcca gtgccaccgc
     4621 ccgttccacg ccgatctgca gcggcgagcc gagccggctg atgtcgatcc cggtcttgcg
     4681 caacagccgt tcgatcggcg tggtggtcac cgtgacgtag cgcgtgatgc ccatccggtc
     4741 ggcgaacgtc acgagttcgt gaattgcctg catcgtcagg tcggcaaagc cgaacgattg
     4801 ctcctcgcca ctggtttcga tggcgaaccg gctcaattcc cagatatgcc gcccgaccgg
     4861 cgcatccgcg ccatgcagca gttgcgggaa ggtgtccttc agcatgttcg gcccctccgt
     4921 cggcatcagg cgccaacagc cccgcacctg gccatcatcg ccctgaatca gcatgtagtg
     4981 cggcccgagc gcgtcgtagc cgtcgatctc catacccgca atggtcggga tgtcccaccc
     5041 gagccgcccg tgaaagaccc gagcccgcag gcggtacatc tcgttgatgt ccgcgttgtc
     5101 aaattcttgc cgcataccaa tccggatcgc tgtttgcatg acgttctcct gaacgtgttg
     5161 gaatacccaa tacgcaagga aacttatgca ttttgaatcg ttctgccacc tacctacctg
     5221 gataggtgtg catgcttatc ggataacgca gaattcgaga taagcctcag ccctcaaccg
     5281 acgggaccca aaatggaact tctcgaaaat cactggcaag cacaaccctc tgtccaaacc
     5341 cgtccggtgg aaccttcatc ggcggtggat cgcgttctga tcattgccta ccggaagaag
     5401 aaaaaagaga gccagcgccg cttctgggca cgcttcggcg tgacgcagtc acgaggtagc
     5461 cggttcgaat cgggcgccga gattccggca ccggtctcga tcctgctggg tctctacttc
     5521 accaaaactg tttccgacgc cgatctgggc cgcgccgagc gggtgctgta cagccgcgac
     5581 gcagccgcct tgctcaaccc gggtcaataa gccccaactg cgcggcgatc acggccgccg
     5641 cgcgtcgcga attcacaccg aacttggtcc tgatgttctt catgtggaag ttcaccacgg
     5701 cttcagaaca gctcaggata tgggagattt cccatgtgga tttgccgcgc gcggtccatt
     5761 tcagacattc gcgttcgcgc ggcgtgagct tgggcaataa ggtctgcgcg tgcgtattca
     5821 aatggcgctg actggtatcg atcaccagat cgcgcagcaa caccagattt ggcaatgcaa
     5881 cgttgacatg gcgccagaag gcgtcggttg ggttgctgtc gttgacaaag cacatcatgc
     5941 cggcttcctg gttcggccca tgaatcggca atgtcacacc cgcgcgcagg ccgtgcgccc
     6001 gggcttcctc gtacatggac tgctgcggcg cggtcgtaaa aatgtccggc gaccagatca
     6061 gcggcgcggc gcgcgtcgcg caatgcgcaa cagtcggatc gatgtggacg agcccctgct
     6121 cgtcgtaggt gcgccgccac gtcggcgagt aggtgctgcg cacataggcg tcttcaaggc
     6181 gaatggtcgg acgcggcagc atggcgatca aaagcctgtc gaaaccccac gtgtcggcaa
     6241 ggcccgcgat tgcactgaac cattccgcct catccgcggc gttcagtaaa ggcgccattt
     6301 cctcgataaa atgtagcgac aatttagatc tcttggactc gcaaacatcc cgttgcgttt
     6361 aattgcggta cggcattccg tccggctctt tttatcttca caatttaaca ctaaaaatca
     6421 tttttcaagc cgagggcgat ttctaaaaac agtaccagcg cgggtccaac gaaccagcga
     6481 acgactctta taacggattt tttgatccga ttaacattca cgggtcgata aagtgggtaa
     6541 ttttctcgta gtagcggttt tcatgatttt ctgattggca atgttattgt caaaccaatt
     6601 ttaatgtggt gtatgtcgaa aatttcgggc gaaactatgc taattgccga attggcaaac
     6661 tccgcttaac cctccgttat tcacaaacat cccctgttgc gcgctattgt catataaaag
     6721 caacgcccct gcaggttgcc aacgtcgctt tcccgccttg acgccgaccc agataacgcg
     6781 atggcagcag tgaccaagtc gtttgacaac atttgcgacg ctgcgacaat cccgcggacg
     6841 gtcctcatcc gccccgcctg ccgcaatccg tgactacggc gcaagggctc gaaaccttgc
     6901 gcgacgaaat tcgctgatcc gcgtgctgca cgacgcggcc acgttctcca aggttgccgg
     6961 catggggccg atcctgccgc tacgcagttc gacgccgccc gaacatcatg cgaagattga
     7021 ccctgcgacg caggtcctgc ttgcattgga gcatcagttc gcgcagaaac ccgcgttgca
     7081 gcgatgtcgt accgacgtcc cgttagcgtc gatcacgctt gcctgcgccg cagcatatcg
     7141 gcatgccgga cgcaacgcgc tgccgcctgc tttccccatc cactttcatt acccaaagcc
     7201 tacgtatgac cgacaacaac cgccgctatc ccgatccctc catccgtgtc ctcgatccgc
     7261 gtttcaagtc gctgatcctc gcgtccgctt ccgtcgagtg tctgtatcag ggcgcgcgct
     7321 ggtcggaagg cccggtctgg ttcggcgacg gccgctatct gctgtggagc gacatcccca
     7381 acgaccgcat cctgcgctgg gacgaaacca gcggtgccgt gagcacgttt cgccaatcgt
     7441 cgaacaatgc gaacggccat acgcgcgacc gccagggccg cctcgtcagt tgcgagcacc
     7501 tgacgcgccg tgtcacgcgc acggagtacg acggctcgat caccgtgctc gccgaccgtt
     7561 atcgcggcaa gcgtttcaat tcgccgaatg acgttgtcgt gaagtcggac ggttcgatct
     7621 ggttcagcga tccgaccttc ggcatcgacg gcttttatga gggcgagcgg caggaatcgg
     7681 aactgcccgc gtgtgtgtat cgcatcgacg gccaatccgg cgaagtgacg gtagtcgccg
     7741 acgacatact gggcccgaac ggcctcgctt tttcaccgga tgaatcggtg ctgtacatcg
     7801 tcgaatcgcg tggcgcgccg cgcaagatcc gtgcgttcga cgtcgacgca ggcggcgata
     7861 gcgccgcgct gtcgaacaac cgtgtgctga tcgacgccgg cccgggcacg ccggatggct
     7921 ttcgcgtcga cgttcacggc aatctctggt gcggctgggg catgggcacc gacgaactcg
     7981 acggcgtgcg tgtcttcacg ccgcagggcg agccgatcgg ccatatcgcg ttgccggaac
     8041 gctgcgcgaa cgtgtgcttc ggcggacgcc atcgcaaccg gttgttcatg gcggcaagcc
     8101 acgggttgta ttcgctgaat gtgaatacgc agggcgttca gggtggatga gtgcgtgggt
     8161 gagtgcgggc ggcctgcacg gccagcgtaa agtgtgactt gcccgacaac ccgctgcgcc
     8221 actcgcctgc cgactccggg tttgcccttg tataaaacgc gcaaaccatt gaaccacaag
     8281 gattgcgtaa caaaatggcc gatttcgtgc accgcacacc gcggcgagta tttgcttgac
     8341 atcaacagtt ccgcttcgct atgttccatt ggtagataga caccgtcggc agcgcatctg
     8401 accggcggac ttccatacca agaagcgagg agacgcaatg actgtttcag gcaggttggc
     8461 gctcagactg gtgtccgtat gtctcgtggc aagcgccgca tgtgcatccg cggcgcacgc
     8521 cgcggatccg gtaacgctca atatcgtcga tgtggccggc gatcttcagc tcacgcaaaa
     8581 gggtttcgag gccttcaagg cgaagtatcc gaatctcgtc gacaatctca cgttcaccaa
     8641 cgcgcccgct ccgcagctgc ccggcaagat caaggcgatg cagggcgccg gccgctccga
     8701 tatcgatctc gtcctgaccg gcaccgacgc gctggcggcc ggcatcgaac agaatctctg
     8761 gataaagcta ttgccggaca atgcagcggc attcccgggc gtgctcgaca aatacgcgcc
     8821 cggtccgcgc aggatgcagg acctcgcaca aggcttcggc ctcgaagtcg catatatgcc
     8881 ggcaggcccg ctgctcgaat acaaccccgc caaggtcagc
//
LOCUS       DATASH010000514         3645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4633327,
            whole genome shotgun sequence.
ACCESSION   DATASH010000514 DATASH010000000
VERSION     DATASH010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4633327"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VHV99_14220"
     CDS             <1..211
                     /locus_tag="VHV99_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012492561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3636613.1"
                     /translation="IRQMGAAGFSQSQAYGLFNTMATQQAAQLGVNDLFFVSAGIFVA
                     LIALIWITRPERAGGDAGAAAAAAH"
     gene            complement(329..1264)
                     /gene="truB"
                     /locus_tag="VHV99_14225"
     CDS             complement(329..1264)
                     /gene="truB"
                     /locus_tag="VHV99_14225"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX3636614.1"
                     /translation="MTAPQRPRVPRRALEGVLLLDKPLGLSSNDALIRAKRLYLAKKA
                     GHTGTLDPLATGLLPLCFGEATKFSQDLLEADKTYEATMRLGIRTTTGDAEGEAIDTR
                     DVTCDQAAVQAAMQKFLGDIVQVPPMYSALKRDGKPLYEYARAGQTVEREGRQVTIHA
                     LDMLACAWPDVTFRVTCSKGTYVRTLAEDIGEALGCGAHLVALRRTGVGALTLANSVT
                     LDALSDATESERDTWLQPVDALLSTFPLVKLDEDATRRFLHGQRLKLSELTIAADAVN
                     APRVRVYAADGRLLGVAKQGEGVLAPERLVVTATN"
     gene            complement(1371..1736)
                     /gene="rbfA"
                     /locus_tag="VHV99_14230"
     CDS             complement(1371..1736)
                     /gene="rbfA"
                     /locus_tag="VHV99_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX3636615.1"
                     /translation="MPKKRSSPNRNVQIADQIQRDLSELLREVKDPRIGLVTIQSVEL
                     TPDYAHAKVYFTTLTGDPQQTLEALQHAAGHLHNQLFKRLHIHTVPTLHFHYDQTIER
                     AVEMSRLIDEANANRAKED"
     gene            complement(1963..>3645)
                     /gene="infB"
                     /locus_tag="VHV99_14235"
     CDS             complement(1963..>3645)
                     /gene="infB"
                     /locus_tag="VHV99_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX3636616.1"
                     /translation="SEVIKSMMKLGQMVTINQMLDQETAMIIVEELGHHAVAAKLDDP
                     EAMLVEGEITDAEALPRPPVVTVMGHVDHGKTSLLDYIRRAKVAAGEAGGITQHIGAY
                     HVETPRGVITFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTKEAIAHAKAG
                     GVPLVVAINKIDKPDANPDRVKQELVAEGVVPEEYGGDSPFVSVSAKTGAGIDELLEN
                     VLLQAEVLELKAPVEAPAKGLVIEAKLDKGKGPVATILVQSGTLNRGDVVLAGSAYGR
                     VRAMLDETGKPTKSAGPSIPVEIQGLSEVPQAGEEVIVMPDDRKAREVALFRQGKFRD
                     VKLAKQQAAKLENMLEQMGEGEVAYMPLIVKADVQGSQEALVQSLLKLSTDEVRVQIV
                     HGAVGGISESDVNLATASKAVIIGFNTRADAQARKLAESNGVDIRYYNIIYDAVDEVK
                     AAMSGMLAPEKREVVTGTVEVRQVFKVPKIGAVAGCMVTDGFVKRSSSVRVLRNNVVI
                     FTGELDSLKRFKDDVKEVRQGFECGMSIKNFNDIVEGDQFEVFEVTEVARSL"
BASE COUNT          652 a         1164 c         1094 g          735 t
ORIGIN      
        1 gatccggcag atgggcgcag cgggcttcag tcagtcgcaa gcttatggcc tcttcaacac
       61 gatggccacg cagcaggccg cgcagttggg tgtgaacgat ctgttctttg tctcggccgg
      121 gattttcgtc gcgctgattg cgctgatctg gattaccagg ccggaacgtg ccggcggtga
      181 tgcgggcgcg gcggccgcgg cggcgcattg agattttgcg caccgatgcc catccggctt
      241 gatgccaccg cgatactaac gacctcgtag cggccacgat gccgattttt ggcccaactc
      301 gctgccgtga cggcgctgaa tttacgcgct agttcgtggc tgtaaccacg agtcgttccg
      361 gcgccaatac accctcacct tgcttcgcga cgccaagcag acgcccgtcc gctgcgtaca
      421 cgcgcacgcg cggcgcattc accgcgtcag cggcgatggt caactcggag agcttcagac
      481 gttgtccgtg cagaaagcgg cgcgtcgcat cttcatccag cttgacgagc gggaacgtgg
      541 acaacaatgc atcgaccggc tgcagccacg tatcgcgctc gctttccgtc gcgtcggaca
      601 acgcatcgag cgtcaccgaa ttcgcaagcg tcagcgcgcc gacaccggta cgccgcagcg
      661 cgaccagatg cgcgccacac ccgagcgctt ccccaatatc ctcggcgagc gtgcgcacat
      721 aggtgccctt gctgcatgtc acgcgaaatg tcacgtcggg ccatgcacag gccaacatgt
      781 cgagcgcgtg aatcgtcacc tgacgccctt cccgctcgac ggtctggcca gcgcgcgcgt
      841 attcgtacag cggcttgccg tcgcgcttga gcgccgaata catcggcggg acctgaacga
      901 tgtcgccgag aaatttctgc atggccgcct gaaccgccgc ctgatcgcac gtcacgtcgc
      961 gcgtgtcgat cgcttcgcct tcggcgtcgc cggtcgtcgt gcggatgccg aggcgcatgg
     1021 tcgcctcata ggtcttgtcg gcttcgagca gatcttgcga aaacttggtg gcttcgccga
     1081 aacacagcgg caaaagaccc gtggcgaggg gatcaagcgt gccggtgtgg cccgcctttt
     1141 tcgccagata gagacgcttc gcgcgaatta gcgcgtcgtt gctcgacagg ccgagcggct
     1201 tgtcgagcag cagcacgccc tcgagcgcgc gacgcggcac gcgggggcgt tgaggtgcgg
     1261 tcatgtcaga aagacagagt cttcgaaggg tacggaaaca gcgctaaacg agtggcaaca
     1321 gaacaattgc gactgagaca ttgtggcgag gaacgcggat agcggcgcgc tcagtcttct
     1381 ttcgcgcgat tggcgttcgc ctcgtcgatc agacgggaca tttcaacggc ccgttcgatc
     1441 gtttggtcgt agtggaaatg cagcgtcggc acggtatgga tgtgcagtcg cttgaacagc
     1501 tggttgtgca gatggccggc cgcgtgctga agcgcttcga gtgtctgttg cgggtcgccg
     1561 gtcagcgtcg tgaagtaaac cttcgcgtgc gcgtagtcgg gcgtcagctc gacgctttga
     1621 atcgtgacga ggccgatgcg cggatccttg acctcgcgca gcagctcgga caggtcgcgc
     1681 tggatctgat cggcgatctg cacgttgcga ttgggagaag aacgtttttt aggcatggtg
     1741 attgtgtgat ccgttcaaac ggatgcaaaa agttgttcgc gcgacagccg cggcggttgc
     1801 tatgtgagcg gcacgcgagc ggcatgcaag cgacccgata gcaacgctaa accaccgcgg
     1861 gggcgctctg cgcgacaaat gcaacaacgc gcgccggccc aaaaacaacg ggcggagcgg
     1921 gcgttaaagc ccgctccgcc cgttttagcc gtgccgcgtg aattacagcg aacgcgcgac
     1981 ttcggtaacc tcgaagactt cgaactgatc gccttcgacg atatcgttga agttcttgat
     2041 cgacataccg cactcgaagc cctgacggac ttccttgacg tcgtccttga agcgcttgag
     2101 cgaatcgagc tcgcccgtga agatgacgac gttgttgcgc aacacgcgta ccgacgacga
     2161 gcgcttgacg aaaccgtccg tgaccataca gccggccacc gcgccgatct tcggcacctt
     2221 gaagacctga cgcacttcaa ccgtacccgt cacgacttcg cgcttctccg gtgccagcat
     2281 gcccgacatc gcggccttca cttcatccac tgcgtcatag atgatgttgt agtagcgaat
     2341 atcaacaccg ttgctttccg ccagcttgcg cgcctgtgca tccgcacggg tgttgaagcc
     2401 gatgatgacc gccttcgaag ccgtagccag gttgacgtcc gactcgctga tgccacccac
     2461 ggcgccgtgc acgatctgca cgcgaacttc gtcggtcgac agcttgagca gcgactgcac
     2521 caaagcttcc tgcgaacctt gcacgtcggc cttgacgatg agcggcatgt acgcgacttc
     2581 gccttcgccc atctgttcca gcatgttctc gagcttcgcg gcctgttgct tggccagctt
     2641 gacgtcgcgg aacttgcctt gacggaacag cgcaacttca cgcgccttgc ggtcgtccgg
     2701 catgacgatg acttcttcgc ctgcttgcgg cacttccgac agaccttgaa tctcaaccgg
     2761 gatcgacggg cctgccgact tggtcggctt gccggtttcg tcgagcatgg ctcgcacacg
     2821 accgtaagcg ctaccagcca gcaccacgtc gccgcggtta agcgtacccg actggaccag
     2881 gatcgttgca accggaccct tacccttatc gagcttcgct tcaatgacga gacccttggc
     2941 cggcgcttcg accggcgcct tcagttccag cacttcggct tgcagcagca cgttttccag
     3001 cagctcgtcg atacccgcgc cggtcttcgc cgacactgac acgaacggcg aatcgccacc
     3061 gtactcttcc ggcacgacgc cttccgcgac gagttcctgc ttcacgcggt ccggattcgc
     3121 atccggcttg tcgatcttgt tgatcgcgac gacgagcggc acgccgcctg ccttcgcgtg
     3181 ggcaatcgct tccttcgttt gcggcatcac gccgtcgtcg gctgcgacca ccagaatcac
     3241 gatgtcggtt gccttcgcac cacgagcacg catggccgta aatgcttcgt ggcccggcgt
     3301 atcgaggaac gtgatgaccc cgcgcggcgt ttcgacgtga taggcgccga tgtgctgcgt
     3361 gatcccgccc gcttcacccg ctgccacctt ggcgcggcga atgtagtcga gcagcgacgt
     3421 cttgccgtgg tcgacgtgac ccatgaccgt gacgaccggc ggacgcggca gtgcttctgc
     3481 gtccgtgatt tcgccttcga ccagcatggc ttccggatcg tccagcttgg ccgcaaccgc
     3541 gtggtggccc agttcctcga cgatgatcat cgccgtttcc tggtccagca tctggttgat
     3601 cgtgaccatc tggccgagct tcatcatcga cttgatgact tccga
//
LOCUS       DATASH010000515         4570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_7005791,
            whole genome shotgun sequence.
ACCESSION   DATASH010000515 DATASH010000000
VERSION     DATASH010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4570
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7005791"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            289..1002
                     /locus_tag="VHV99_14240"
     CDS             289..1002
                     /locus_tag="VHV99_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009769518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HEX3636617.1"
                     /translation="MDLERILFTQGFGSRRQCRALIADGRVSIAGSPCTNAALEIASD
                     ELASFEFQVDGATWRYREKAYLALNKPPGYECSRDPQHHLSVFHLLPPQLTERGVQCV
                     GRLDQDTTGLLLLSDDGAFVHAFTSPKRKVPKVYLATTRYPLDDAQLSALRNGVLLHG
                     ESQPIPAVSAEQRDERQLELSVLEGKYHQVKRMVAAAGNRVEKLHRERIGGFALPDEL
                     AEGQWRWLDEADLALLRHC"
     gene            1147..2013
                     /locus_tag="VHV99_14245"
     CDS             1147..2013
                     /locus_tag="VHV99_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016344519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3636618.1"
                     /translation="MPAQQKRRIVTFGLASLGAAALAACSPVKLLNATIPSSGYRRTS
                     GIAYGSDPRQVLDVYVPAAASTQPRPVVVFFYGGSWQTGDRANYLFVAQALTSRGYVA
                     VLPDYRTYPETAFPGFVNDAAAAARWTRDHAHEFGADPLRLFVMGHSAGAHLAALIAT
                     DPQYLASQSMSKADLRGVIGLAGPYDFLPLTDRTLFDVFPAGKRAESQPINYVMGHEP
                     PMFLAAGTDDTVVDPGNTDRLAAVLRKHGDSVEVKHYDGFGHIRIVSALALPLRGSST
                     VLADLSSFINSR"
     gene            2147..2323
                     /locus_tag="VHV99_14250"
     CDS             2147..2323
                     /locus_tag="VHV99_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636619.1"
                     /translation="MAAHMTIAMIAFVAISVLFIVGLLVTMHLRHRYQPNLIGALIGA
                     LLCFILLETLPVLM"
     gene            complement(2289..3401)
                     /locus_tag="VHV99_14255"
     CDS             complement(2289..3401)
                     /locus_tag="VHV99_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX3636620.1"
                     /translation="MIATRKLRRARVLIAGCGDVGLRCVPLLAHRAAAPRVIALSSQP
                     ARAAELRAAGTTPLIGNLDERRSLRRLAGLASTVLHLAPPQKTGDDDSRTRALIAALS
                     APRRSVAREATSAVARQRAWRGWFRATFKSGTPAAVKTHAKAIKRASIVPDRVSSAAA
                     RRPQIRLIYASTSGVYGDCGGAWIDETRPVRPDNARAKRRVSAETQLRGATRRGVLSA
                     SIVRIPGIYAGNRLPLARLHKGTPALAPADDVYTNHIHADDLAAILVLAIRRARPQRV
                     VHASDDSDLRMGDYFDLVADAFGLPRAPRVSREQAEAQLEPMMLSFMRESRRLQNTRL
                     RDEMRYALRYPSIDDFLRQANVDPAVTSEPVASPAG"
     gene            3461..4492
                     /locus_tag="VHV99_14260"
     CDS             3461..4492
                     /locus_tag="VHV99_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006413312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HEX3636621.1"
                     /translation="MAFNVTLRQSGRQFQVEPDEAVLLAALRQGIGLPYGCKNGACGS
                     CKGTVVSGEIEQAPHSSSALSNDEKTRGMALLCCATAKGDLTVDIREVAGVGDIQVKK
                     LPCRVNALDRKAADVIELKLQLPANERLQYLAGQYIEFILKDGKRRSYSMASPPHHEG
                     PIELHVRHMPGGTFTDHVFGPMKERDILRFEGPLGTFFLREDSEKPIVLLASGTGFAP
                     IKAIIEHAVFKNLNRPMTLYWGGRRKQDLYMMDMAEQWAKDVPDFSFVPVLSEPDATD
                     AWTGRTGFVHRAVIEDLPDLSAYQVYACGAPVMVESAQRDFTSHHRLPEDEFYADSFT
                     SAADLANPV"
BASE COUNT          868 a         1412 c         1389 g          901 t
ORIGIN      
        1 gtcctttcgt catacgcgtg gaacgggtca aaacggctcg aagcgaaaag cataaacgcc
       61 atgcaggcga ccaactatga aaagacctta atcggcctgc tttacgaatg gccgacgaca
      121 gcggctgagg gccgatatgc cggtgtgcac cgtgttcata cggccctcaa ttcatgacgg
      181 gatcaacata aagctcagcg tggttcaggc agccgccgtg cgggatcaaa gcacgccggt
      241 tgctagaatg tccgtccctt ctttctgcct gagccaagaa ccgccctgat ggatctcgaa
      301 cgcattcttt tcacgcaggg tttcggctcg cgccgccaat gccgcgcgct tatagccgat
      361 ggccgcgtct ccatcgcggg ctcgccgtgt actaatgctg cgctcgagat cgcgtccgac
      421 gagctcgctt cgttcgagtt tcaagtcgat ggcgcgacat ggcggtatcg ggaaaaagcg
      481 tatctggcgc tgaacaaacc gccgggctac gagtgctcac gcgatccgca gcaccatctg
      541 agcgtctttc accttttacc cccgcaattg accgagcgtg gcgtgcaatg cgtgggacgt
      601 ctggaccagg acacgaccgg ccttctgctc ctctccgatg acggcgcgtt cgtgcatgcg
      661 ttcacatcgc cgaaacgcaa ggtgccgaag gtgtatctcg cgaccacgcg atatccgcta
      721 gacgatgctc aactctcggc acttcgcaac ggcgtcttgc tgcatggcga gtcgcagcca
      781 atcccggccg ttagcgctga acaacgcgac gagcgacagc tcgagctgtc ggtgctcgaa
      841 ggcaagtatc accaggtgaa gcggatggtc gcagcagcgg ggaaccgcgt ggagaaattg
      901 catcgggagc gtattggcgg gttcgctctg cccgatgagc tggccgaagg ccaatggcgc
      961 tggctggacg aggcagacct cgccctgctc aggcattgct gaggcgcttc gtgtgtcata
     1021 tccgtgcgac agcgcgctga gtcgcggtca cgtcgggttt aacgtctcaa tcaggccaac
     1081 gcggcgcgct gcatccatcc gggcgcttcg agcgtagaag tggcatccgc ctgcttttgg
     1141 acatttatgc ccgcacaaca aaaacgccgc atcgtgacat tcggtctcgc cagtcttggc
     1201 gctgcggcgc tcgccgcgtg cagtcccgtc aaacttttga acgcgaccat tccgtcgagt
     1261 ggctacagga gaacatcagg tattgcgtac ggaagcgatc cgcgccaggt actcgatgtc
     1321 tacgtacccg ccgccgcaag tactcaaccg cgtccggtcg tcgtcttttt ctacggcgga
     1381 agctggcaga ccggcgatcg tgccaactat ctgtttgtcg cacaggcctt gacgtcgcgc
     1441 ggctacgtgg cggtgctgcc ggactaccgc acgtatccgg aaacagcgtt tcctggtttt
     1501 gtgaacgatg cggctgccgc cgcgcgctgg acgcgcgacc acgcgcacga attcggcgca
     1561 gatccgttac ggctttttgt aatgggacat tcagcaggcg ctcatcttgc cgcactgatc
     1621 gcgaccgatc cgcaatatct cgcttcacaa tcgatgtcga aagccgactt gcgcggggtg
     1681 atcggcctgg ctggacctta cgatttcctg ccgctcacgg atcgcacgct attcgatgtc
     1741 ttcccggcag gcaagcgcgc cgagagccag ccgatcaact acgtcatggg gcacgagccg
     1801 ccgatgttcc tcgcggccgg cacggacgac accgtcgtcg atcccggcaa caccgaccgt
     1861 ctggcagccg tgttgcgcaa acatggcgac tcggtggaag tgaaacatta cgacggtttc
     1921 ggccatattc gtattgtttc cgcactggcg ctgccgttgc gcggcagctc gacggtgctc
     1981 gcggatcttt cgtcgttcat caactcgcga taagcgccgc gagacccata ccgtggcgat
     2041 tcaataggtg ttaacacagc acgctggcgc cgcaccgcaa catgcctttc ttacgacgaa
     2101 aatctatact cctttcacga ctattaaaag aagcaggagg gccaccatgg ccgctcacat
     2161 gaccattgca atgattgcgt tcgtggcgat cagcgtgttg tttatcgtcg gattgctggt
     2221 gacgatgcat ctgcgccatc gctatcaacc caacctgatt ggtgcgctga tcggggcctt
     2281 gctgtgcttc atcctgctgg agacgctacc ggttctgatg tgacggctgg atcaacatta
     2341 gcctggcgca ggaagtcatc aatgctcgga taacgcagcg cgtaacgcat ttcgtctcgc
     2401 aaccgggtgt tctgcagacg tcgcgattcg cgcatgaacg agagcatcat cggctcgagt
     2461 tgcgcctctg cctgctcgcg tgaaacgcgg ggggcgcgcg gcaagccgaa cgcatcggca
     2521 accaggtcga aataatcgcc catgcgcaag tcgctatcgt ccgatgcatg caccacgcgc
     2581 tgcggccgcg cccgccggat cgcgagcacg agaatggcgg ccaggtcgtc ggcgtggata
     2641 tggttcgtgt agacatcgtc ggctggcgcg agtgccggcg ttcccttgtg cagccgtgcc
     2701 aacggcaggc ggttgccggc gtaaatcccc ggaatccgca caatcgatgc actcagcacg
     2761 ccgcgccgtg tagcgcctcg caattgcgtt tccgccgata cccttcgttt cgcgcgtgcg
     2821 ttgtccggcc tgaccgggcg cgtctcgtcg atccacgccc cgccacaatc gccataaacc
     2881 ccggacgtgc tcgcatagat caggcggatt tgcggccggc gcgcggcggc gctcgaaacc
     2941 cggtcgggta caatagacgc tctttttatg gctttggcgt gtgttttgac ggccgctggc
     3001 gtgccgcttt tgaaggtcgc gcgaaaccat ccgcgccacg ctcgctgacg ggccacagcg
     3061 ctggtcgctt cgcgcgcaac ggaccgtcgc ggcgcactca acgcggcaat cagcgcgcgt
     3121 gtcctgctgt cgtcgtcgcc ggttttctgg ggtggcgcga gatgcagcac cgtggacgca
     3181 agaccggcca gccgtcgcag gctgcggcgc tcgtccaggt tgccaatcaa cggtgttgtg
     3241 ccggctgcgc gcagttcggc ggcgcgtgcg ggttgacttg aaagtgcgat cacgcgaggc
     3301 gcggcggcac ggtgtgcgag aagcggcacg caacgcaggc cgacatcgcc gcaaccggca
     3361 atcagcacgc gtgcgcggcg tagttttcga gtggcgatca tgctgtcgat tgtagccgcg
     3421 acagcttttg catttgtttc atccaacgca ttcaggatct atggctttca acgttacgct
     3481 ccggcaaagc ggccggcagt ttcaggtaga acccgacgaa gcggtgctgc tcgccgccct
     3541 gcgtcagggc atcggtcttc cctacggctg caagaacgga gcctgtggtt catgcaaggg
     3601 cacggtcgtc tccggcgaaa tcgagcaggc gccacattcg tcgtcggcct tgtccaacga
     3661 cgaaaaaacc cgcggcatgg cactgctgtg ctgcgcgacc gccaagggcg acctgacggt
     3721 cgacatacgc gaagtggcag gggtaggcga cattcaggtc aagaagctgc cttgccgcgt
     3781 gaacgcgctg gaccgcaagg cggctgacgt cattgaactg aagctgcaat tgccggccaa
     3841 cgaacggctg caatacctgg ccggccagta catcgaattc atcctgaagg acggcaagcg
     3901 ccgcagctat tcgatggcga gcccgccgca ccacgaaggc ccgatcgaac tgcacgttcg
     3961 ccatatgccc ggcggcacct tcacggacca cgtgttcggc cccatgaaag agcgcgacat
     4021 cctgcgcttc gaaggcccgc tcggcacatt ctttctgcgt gaagattcag agaagcctat
     4081 tgtgctgctc gcgtccggca cgggcttcgc gccaatcaag gcgatcattg aacatgctgt
     4141 gttcaagaac ctgaaccggc ccatgacgct ctattggggc ggccgccgca agcaagacct
     4201 gtacatgatg gacatggccg aacaatgggc gaaggacgtg ccggacttct cgttcgtgcc
     4261 ggtactgtcg gaacccgatg caaccgacgc ctggaccggc cgtacgggct ttgttcatcg
     4321 tgctgtcatt gaagatctgc cagacttgag cgcgtaccag gtgtacgcct gtggcgcgcc
     4381 ggtgatggtg gaatccgcgc agcgtgattt cacgagtcac caccgtttgc cggaggacga
     4441 gttctacgcc gattcgttca ccagcgcggc ggatcttgcg aatccggtct agcctcacgc
     4501 aagccgtact caagccgccg agtgtggcgg ctcgacaaaa aatcgagcgg ccatggttta
     4561 cacgcaggaa
//
LOCUS       DATASH010000516         2956 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3840963,
            whole genome shotgun sequence.
ACCESSION   DATASH010000516 DATASH010000000
VERSION     DATASH010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3840963"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="VHV99_14265"
     CDS             <1..643
                     /locus_tag="VHV99_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007584299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX3636622.1"
                     /translation="VRRRPDIRRAEAQLHAATAEIGVAQADFYPQISLTGSLGFQSLQ
                     LSSLGDWASHQFVFGPSISLPIFQGGRLKGTLELRKAQQQEAAIIFQRTVLQAWHEVD
                     DALSAYDAEQRRRDQLKEVVQQDQVALAVAQQRYREGAIDFLNVLTVQRDLLNAESDL
                     AQSRAIASVNLVILYKALGGGWETAFPEVPPAEERARSAPLKTAHTAANGSGS"
     gene            complement(652..900)
                     /locus_tag="VHV99_14270"
                     /pseudo
     CDS             complement(652..900)
                     /locus_tag="VHV99_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008344483.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1071..2939)
                     /locus_tag="VHV99_14275"
     CDS             complement(1071..2939)
                     /locus_tag="VHV99_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007749145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3636623.1"
                     /translation="MTLGVTVAAFAGLVTFLHWRHEQHNARVSPALNGVASQMRDDAS
                     AWTHQEKDASEMLRDIREQDVTAIGVSPNAILVSTRDGAKYFVTDHNATFSNALLLGE
                     IKADVRSPYQLAWLPDANIRTGTSRWIDAFDKTHDAISLLLPLLLIGGLIWLMRREMK
                     AGAKLLDRAPALCFDDVIGAGEAKAALSDIQAFLTNPKQFTDMGVRAPCGILMVGGPG
                     VGKTRLAQALAGECGANFISITGSYFSAKYYGVGIQKVKHLFDLARKNAPTVIFIDEA
                     DGLAKRTDTGSGPVEAESNRIINQLLAEMDGFASNEGVIIVAATNHPDNLDEALRRPG
                     RFDRTVHVRLPDLEDRAKIFRFYAGKLTSKSDDIDFNQLARLTTGLSPATLAMVVNQA
                     GLVARKVGDDEVASKHFVEAIKIARIGDVSGAERALNEDERTRIAVHEAGHGLVAAVL
                     GTGVLEEVTILPRGGALGVALITKTQDKHLYRETEMRDEIQVLLGGRNAELLAFSEAS
                     SGAAQDLQEASRISLDMVSKFGFNVDGNLFSLSALPAQYAGLQMKTAIEHANILLKHL
                     NEACYALLRDNEPVLRAIAEQLLESETVPGATVYRLIEEHKATKDGLHLAQKPAAA"
BASE COUNT          569 a          927 c          876 g          584 t
ORIGIN      
        1 tgtacgacgg cggccagata tccggcgggc ggaagcccaa ctgcacgccg cgacggcaga
       61 gataggcgtc gcgcaggcgg atttctatcc gcagatctcg ctcacgggca gcctcggttt
      121 tcagtcgctc cagctttcca gcctgggcga ctgggcctca catcagttcg tcttcggtcc
      181 ttcgatctcg ctgcccattt tccagggcgg aaggctcaag ggcacactcg agctgcgcaa
      241 ggctcagcaa caggaagcgg cgatcatttt ccagcgtacg gtgttgcagg cctggcatga
      301 agtggatgac gcactgtcgg cctacgatgc cgagcagcgc cgtcgtgacc agttgaagga
      361 agtggtgcag caggaccagg tcgcgcttgc ggtcgcgcag cagcgctatc gcgaaggtgc
      421 cattgatttc ctcaacgtgc tcacggtgca gcgggacctg ctgaacgccg aaagcgatct
      481 ggcgcagagc cgtgcaatcg cttcggtcaa tctcgtcatc ctctataagg cccttggcgg
      541 tgggtgggag acggccttcc cggaagttcc gccggctgag gagcgcgcta gatccgcgcc
      601 actgaaaacc gcccataccg ctgctaatgg atccggttcg tagattgata atcatcgaaa
      661 agccttctgg ccgcccacaa aagagggcgg ccagggtccg agttgagagg caacgattaa
      721 cccgctaagg agaacgccgg acatagttcc gcgtgggaga tagtcaagct tcgtatagcc
      781 gagcaacagc tgaaccaccc tgaggtgctt tgtgcgacgg tagagcaacg atggcttggt
      841 gcggcgcatg gtatgggttc cgtattccag accgagcgac gcaatgcacc aatgcaccaa
      901 tgcacccact gagcgtactc cgagtcgccc agccgacggt catgcaacag aagacgcaac
      961 ggccggtgca gttcgagatt acagagggac ccgcgacagc gttgaggcgt cgaccaggga
     1021 ggcggggtgt ccggctttcg ttcgaagccg gttcagttca tgggcaacgt tcaggccgcc
     1081 gccggctttt gcgcaagatg caacccgtcc ttcgttgcct tgtgctcttc gatcagccga
     1141 tagacggtcg cgcccggcac cgtctccgat tcgagcagtt gttcggcaat cgcacgcagc
     1201 accggctcgt tgtcgcgcag cagcgcgtag catgcttcgt tcaagtgctt cagcagaatg
     1261 ttcgcgtgct cgatcgcggt tttcatctgc aaaccggcgt actgcgccgg caatgccgaa
     1321 aggctgaaca gattgccatc cacattgaag ccgaacttcg acaccatatc aaggctgatc
     1381 cgcgacgctt cctgcaagtc ctgcgctgcg ccgctcgacg cttccgagaa cgccagcaat
     1441 tccgcattcc gtccgcctag cagcacctga atctcgtcgc gcatttcggt ttcccggtac
     1501 aggtgcttgt cctgcgtctt cgtgatcaga gcgacgccca atgcgccgcc acgcggcaga
     1561 atcgtcacct cctcgagcac gccggtgccg agcaccgcgg cgacgagtcc gtggcccgct
     1621 tcgtgaaccg cgatccgcgt gcgttcgtct tcgttgagcg cgcgctcggc gccgcttacg
     1681 tctccgatgc gcgcaatctt gatcgcctcg acgaaatgct tcgacgccac ctcgtcatcg
     1741 cccactttgc gcgcgaccag tccagcctga ttgacgacca tcgcgagtgt ggccggcgac
     1801 agccccgtcg tcaaacgcgc cagttgattg aagtcgatgt catcggattt cgacgtcagc
     1861 ttcccggcgt agaaacggaa tattttcgcg cggtcttcga gatcgggcag acgcacgtgc
     1921 acggtgcgat cgaaacggcc cggacggcgc aacgcttcat cgagattgtc cggatgattg
     1981 gtagccgcaa cgatgatcac gccttcgttc gacgcaaagc catccatttc cgcaagcaac
     2041 tgattgatga tccggttgct ctccgcttcg acaggaccgc tgcccgtatc cgtgcgtttc
     2101 gcgaggccgt cggcttcgtc gatgaaaatc acggtcggcg cattcttgcg cgcgagatcg
     2161 aatagatgct tgaccttctg aatgccgacg ccgtagtact tcgcgctgaa atagctgccg
     2221 gtaatcgaaa tgaagttggc gccgcattcg cctgcgagcg cctgcgccag gcgcgtcttg
     2281 ccgacgcccg ggccgccgac catcaagata ccgcacggtg cgcgcacgcc catgtcggtg
     2341 aattgcttcg ggttggtcag aaacgcctga atgtcggaga gcgcggcctt cgcttctccc
     2401 gcgccgatca cgtcgtcgaa gcaaagcgcc ggcgcacggt cgagcagctt cgcaccggcc
     2461 ttcatttcac ggcgcatgag ccagatcaat ccaccgatca gcaacagtgg cagcaacagg
     2521 ctaatcgcat cgtgcgtctt gtcgaacgcg tcgatccagc gtgacgtgcc cgtgcgaatg
     2581 ttcgcatccg gcagccacgc gagctgatac ggcgagcgca cgtcggcttt tatttcgcca
     2641 agcagcagtg cattagagaa agtcgcgttg tgatcagtaa cgaagtactt cgcgccgtcg
     2701 cgtgtcgaca ccagaatcgc attcgggctc acgccgatcg ccgtcacgtc ctgctcgcga
     2761 atatcgcgca gcatttccga agcgtctttc tcctgatgcg tccacgccga tgcgtcgtcg
     2821 cgcatctggc tcgcgactcc attgagcgcc ggcgaaaccc gtgcgttatg ttgctcgtgg
     2881 cgccaatgca gaaacgtgac gagtccggcg aaggccgcaa cagtcactcc tagcgtcacg
     2941 taacgtccgt atcggg
//
LOCUS       DATASH010000517         2605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_675164,
            whole genome shotgun sequence.
ACCESSION   DATASH010000517 DATASH010000000
VERSION     DATASH010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675164"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(348..1025)
                     /locus_tag="VHV99_14280"
     CDS             complement(348..1025)
                     /locus_tag="VHV99_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX3636624.1"
                     /translation="MKTTVRSERRGRSGTRGFTLIELLVAIAIMAVIAVLSWRGLDQI
                     IRGRQTITNAMEDERVFAQLFGQMRIDVRQAASDDESGQAAVTVSGNVLQIVRETLLP
                     GTAPRLQVVRYRISEGRVIRYASPPLANVGDLRRALRGGEGDGWTAVPLMGGVGAISA
                     RVYVPKVGWTTQMKDVQSAITENDNNLKVPQLGNAPLPRSVTGLEVSVGAKSLARPIT
                     RVFLVGE"
     gene            complement(1003..1407)
                     /gene="gspI"
                     /locus_tag="VHV99_14285"
     CDS             complement(1003..1407)
                     /gene="gspI"
                     /locus_tag="VHV99_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HEX3636625.1"
                     /translation="MRRCRDMGARVSQRGFTMIEVLVALAIIAIALAASLRAVGSLAS
                     GEADLHQRLLAGWSADNTLAQLHLTHGWPNVGSTSFDCSQGNLQLICTEHVTATPNPV
                     FRRVEVMVTSPGRSGNLAQMVTVVANENNRSL"
     gene            complement(1409..2014)
                     /locus_tag="VHV99_14290"
     CDS             complement(1409..2014)
                     /locus_tag="VHV99_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HEX3636626.1"
                     /translation="MRLSACKSRVDIPCTAQPSDMACAGARMRGSRGSRSSCGSRSSR
                     DSCAGGRKRAAGFTLLEMMVVLVIAGILVSLTALTMTRNPRTDLNEEAQRLALLFESA
                     GDEAQVRARPIAWQPFEGGFRFDVRTEDGWRPLRDDLLGPRSWEGGVTGATIDYPGSD
                     SHPSRVIFGTEAIDVPVEITLFSAVGRATIVGTGNGRYEVH"
     gene            complement(2174..>2605)
                     /gene="gspG"
                     /locus_tag="VHV99_14295"
     CDS             complement(2174..>2605)
                     /gene="gspG"
                     /locus_tag="VHV99_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HEX3636627.1"
                     /translation="AAPRSRRQRGFTLIEIMVVIAILGILAALIVPKIMSRPDEARRV
                     AAKQDIGTVMQALKLYRLDNGRYPTQEQGLRALIEKPSTDPVPNNWKDGGYLERLPND
                     PWGNGYQYLNPGVHGEIDVFSYGADGKAGGEGNDADVGSWQ"
BASE COUNT          567 a          919 c          691 g          428 t
ORIGIN      
        1 actgctttgt cacgagaggt gaatgtgcga gggcacggat tccagatgca ccactgcggt
       61 ttgctgcgtg ggggacccgc ttagcattag cggtttgagc cgctttcttt tgcctacttt
      121 tctttgccgc ggcaaagaaa agtaggtgcc tgccccgcac aggggcgacg ctaatagacc
      181 gataagaaaa caaggaaagg ccaacaccgc gagagcgcag acaaagacgc caacccgcca
      241 gaaagccaaa gaaagccccg agccgcagac aaacagaccc agcgcatcgc gaacacaaca
      301 ccaacgaaga acccgagcgt cgcggagaca aaaaagaacc cctcatttca ttctccgacg
      361 agaaaaaccc gcgtaatagg ccgcgccaac gacttagccc ccacactcac ttccagccca
      421 gtcaccgacc gcggcagcgg cgcattcccc aactggggca ccttcaaatt attatcgttc
      481 tccgtgatcg cgctctgcac atccttcatc tgcgtggtcc agccgacctt cggcacatac
      541 acacgcgccg atatggcacc cacgccgccc atcaagggca ccgccgtcca cccatccccc
      601 tcgccaccgc gcagcgcacg ccgcaagtcc cccacattcg ccaacggtgg cgacgcatac
      661 cgaatcacgc gtccttcgga tatgcgatac ctcaccacct gcaaacgcgg cgccgtcccc
      721 ggcaacaacg tctcccgcac gatctgcagc acgttgccgc tgaccgtcac ggctgcctgt
      781 ccagactcat cgtccgacgc ggcctggcgc acgtcgatcc gcatctggcc gaacaactgc
      841 gcaaagacgc gctcgtcctc catcgcgttc gtaatcgtct gccggccacg aatgatctgg
      901 tccagcccgc gccacgacaa cacggcaatc accgccatga tcgcaatcgc gaccagcaac
      961 tcgatcagcg tgaaaccacg cgtgcccgat cggccgcggc gctcagagcg aacggttgtt
     1021 ttcattcgcg accaccgtga ccatctgggc gagattgccg gaacgccccg gcgacgtcac
     1081 catcacttcc actcgacgaa acaccggatt cggcgtcgcc gttacatgct cggtacagat
     1141 caattgcaaa tttccctgcg agcagtcaaa actcgtcgag ccgacgttcg gccagccatg
     1201 cgtcaaatgc aattgcgcca gcgtattgtc ggcgctccat cccgccagca gccgttgatg
     1261 cagatcggct tcgccgctcg ccagactccc caccgcgcgc aacgacgccg ccaatgcaat
     1321 cgcgatgatc gccagcgcta ccagtacttc gatcatcgta aaaccgcgct gcgatacgcg
     1381 agcgcccatg tccctgcaac gacgcatctc agtgcacctc atagcggcca ttgcccgtgc
     1441 cgacgatcgt cgcgcgcccc accgccgaaa acagcgtgat ctccacgggc acgtcgatgg
     1501 cttctgtgcc gaatatgacg cggctcgggt gcgaatccga cccgggatag tcgatcgtgg
     1561 cacccgtcac gccgccttcc cagctacgcg gcccgagcag gtcgtcacgc aacggacgcc
     1621 agccgtcttc ggtacgcacg tcgaaacgaa aaccgccctc gaacggctgc caggcgatcg
     1681 gccgtgcgcg cacctgggct tcgtcgccgg ccgattcgaa tagcagcgcc agacgctgcg
     1741 cctcctcatt cagatcggtg cgcgggttgc gggtcatcgt cagcgcggtc agcgatacca
     1801 gaatgcccgc gatgaccagc accaccatca tttccagcag cgtgaacccc gccgcacgct
     1861 tgcggccacc tgcgcacgaa tcacgtgagc tacgtgagcc acatgaacta cgcgacccac
     1921 gtgagccgcg catgcgtgcg ccagcgcacg ccatgtccga cggctgagcc gtacacggaa
     1981 tgtccacacg ggacttgcaa gcggacagtc gcataatgcc ggccacgaac gatcaaccaa
     2041 aaatcagaat aacaatgagt cgataagacg cgcggcggat cgcaaaagag gagggcgatc
     2101 cggcgcgcct gcaaaccaac ggcgcttaat agacgactga gacgaagacg actgagacga
     2161 ctggcctacc ggactactgc cacgacccga catccgcgtc gttgccttcg ccgcccgctt
     2221 taccgtccgc gccatagctg aacacatcga tttcgccatg cacgcccggg ttcagatact
     2281 gatagccatt gccccacgga tcgttcggca gacgttcgag gtaaccgccg tccttccagt
     2341 tgttcggcac cgggtccgtg ctcggctttt cgatcagcgc gcgcagacct tgctcctgag
     2401 tcggataacg gccgttgtcg agccgataga gtttcagagc ctgcatcacc gtgccgatgt
     2461 cctgcttggc cgcgacgcgc cgcgcttcgt ctggacggct catgattttc ggcacgatca
     2521 acgccgccag aatgcccaga atcgcgatca cgaccatgat ttcgatcagc gtgaaaccgc
     2581 gttgacgacg gctgcgcgga gccgc
//
LOCUS       DATASH010000518         3151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4436555,
            whole genome shotgun sequence.
ACCESSION   DATASH010000518 DATASH010000000
VERSION     DATASH010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4436555"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..1543)
                     /locus_tag="VHV99_14300"
     CDS             complement(50..1543)
                     /locus_tag="VHV99_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009913510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX3636628.1"
                     /translation="MNAPVELTAEVLAQRQREVVQALMAVLPTHCLLYREEDTVAYEC
                     DGLAAYRRLPLAVALPETESQVQRIVQICHRLDVPIVPRGAGTGLSGGAMPIRHGVVV
                     SLARFRKIVEVDSYARTATVQPGVRNLSISEAAAPYGLYYAPDPSSQIACTIGGNVAE
                     NSGGVHCLKYGLTVHNVLRVRAVTMEGEVVEFGSLAPDAPGLDLLAVLIGSEGMFAIV
                     TEVTVKLIPKPQTAQVIMASFDDVVKGGDAVAGIIAAGIIPAGLEMMDKPATRAVEEF
                     VNAGYDLDAAAILLCESDGTSEEVADEIVRMTAVLREQGATRIQISRSENERLRFWSG
                     RKNAFPAAGRISPDYYCMDGTVPRRSIGPLLTRIEVMEKKYGLRCINVFHAGDGNMHP
                     LILFNGNDMDEWHRAEAFGCDILEACVELGGTVTGEHGVGIEKINSMCVQFSPEERDT
                     FHAVKRAFDAPGLLNPDKGIPTRARCAEYGKMHVRGGLLPHPELPRF"
     gene            complement(1673..3088)
                     /locus_tag="VHV99_14305"
     CDS             complement(1673..3088)
                     /locus_tag="VHV99_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX3636629.1"
                     /translation="MNHPVPPAPLRRPFPAELLTALKAAFGERVSTAEAVRAHHGRDE
                     SPFDPQLPDAVVFARNTEDVQTIVKLCGQYNVPIIPYGNGSSLEGHLLAVQGGVSIDL
                     SEMNRVLSINAEDLTVTVEPGISRKQLNEALRDTGLFFPIDPGADASIGGMSATRASG
                     TNAVRYGTMRENVLGLTVVLADGRMIKTGTRARKSSAGYDLTRLFVGSEGTLGVITEI
                     TVRLYPQPEAVSAAVCTFPSMGDAVRAVIETIQMGVPIARVEFVDSLAIRSINRHSNL
                     TLREAPTLFFEFHGTEAGVKEQAELVQELAAQNAGEGFEWATRPEDRSRLWNARHNAY
                     FAMLQLKPGCRAVTTDVCVPISRLAECVVETEQDLNASPLPCPIVGHVGDGNFHVAIL
                     IDPNKPEELAEAERLNHRIVQRALRMDGTCTGEHGVGLHKMNFLLEEHGEVAVDTMRS
                     IKHALDPRNLMNPGKIFSWAA"
BASE COUNT          545 a          979 c         1023 g          604 t
ORIGIN      
        1 tgcggctcat ggattgttta tgtgccgcct gcgtgtcgct caagtgcccc taaaaacgcg
       61 gcaattccgg gtgaggcaac aaaccgccgc gcacgtgcat cttgccgtac tcggcgcagc
      121 gggcgcgggt ggggatgccc ttgtcgggat tgagcaggcc cggcgcgtca aaggcgcgtt
      181 tgaccgcgtg gaacgtgtcg cgttcttcgg gtgagaactg cacacacatc gaattgattt
      241 tttcgatgcc cacgccatgc tcgccggtca ccgtgccgcc cagttcgacg cacgcttcga
      301 gaatgtcgca accgaacgcc tcagcgcgat gccactcgtc catatcgttg ccgttgaaca
      361 gaattagcgg atgcatgttg ccgtcgccgg cgtggaacac gttgatgcag cgcagcccgt
      421 atttcttctc catcacttcg atgcgcgtca gcagcggccc gatactacgg cgcggcacgg
      481 tgccgtccat gcagtaatag tcgggtgaaa tgcggccggc ggccggaaac gcgttcttgc
      541 gccccgacca gaagcgcagc cgttcatttt ccgagcgcga aatctggatg cgggttgcgc
      601 cctgttcgcg caacacggcg gtcatgcgca cgatttcgtc ggcgacttct tcagacgtgc
      661 cgtccgattc gcacagcagg atcgccgccg cgtcgaggtc gtagcccgca ttgacgaact
      721 cctcgacggc gcgcgtggcc ggtttgtcca tcatctccag accggccgga atgatgcccg
      781 ctgcaatgat gccggcgacc gcgtcgccgc ctttcacgac gtcgtcgaaa ctggccatga
      841 tgacctgcgc cgtttgcggc ttcgggatca gcttgacggt gacttccgtg acgatcgcga
      901 acatgccttc gctgccgatc agcacagcga gcagatcgag ccccggcgcg tcgggcgcga
      961 gcgaaccgaa ttcgacgact tcgccttcca tggtcacggc gcgcacgcgc agcacgttgt
     1021 ggacggtgag gccgtatttg aggcagtgca cgccgcccga attttcggcg acgttgccgc
     1081 cgatcgtgca ggcgatttgc gatgagggat cgggggcgta gtaaaggccg tatggcgcgg
     1141 cggcttcgga aatcgacaga ttgcgcacgc ccggctgcac agtcgcggta cgcgcatacg
     1201 aatcgacttc gacgatcttg cgaaagcgcg cgagcgacac caccacgccg tgacgaatcg
     1261 gcatcgctcc accggacaag ccggtgcccg cgccgcgcgg cacgatcggc acgtcgagcc
     1321 ggtgacagat ctgcacgatg cgctgtacct gcgattccgt ttccggcaat gcgaccgcga
     1381 gcggcagacg ccggtaagcg gcgagaccgt cgcactcgta ggcaacggtg tcttcttcgc
     1441 gatacagcag acagtgggtg ggcagcacgg ccatcagcgc ttgcacgact tcgcgctggc
     1501 gctgggcgag aacttcggcc gtcagttcga cgggtgcgtt catatgactt gtctcctcgt
     1561 gtctttcgcg ctgcgtgccg ggtcaagccc tgcttgtgtg gcagcaggcg ttgccgctgc
     1621 ccgctgcctg ctgctgtaag tgagctgagc cgaactgggg ttcgacgacg ccctatgccg
     1681 cccaggaaaa gatcttgccc gggttcatca gattgcgcgg atcgagcgcg tgtttgatgg
     1741 agcgcatcgt atcgacggcg acttcgccgt gttcctcgag caggaagttc atcttatgca
     1801 gaccgacgcc gtgctcgccc gtgcaggtgc cgtccatgcg cagcgcgcgt tgcacgatgc
     1861 gatggttcag gcgctcggct tcggcgagct cctccggctt gttcggatcg atcaggatcg
     1921 cgacgtggaa gttgccgtcg ccgacatggc cgacgatcgg gcagggcagc ggcgacgcgt
     1981 tcagatcctg ctccgtttcc acgacgcatt cggcgaggcg cgagatcggc acgcagacat
     2041 cggtcgtcac ggcgcggcag ccgggcttca gttgcagcat cgcgaaatag gcgttgtggc
     2101 gcgcgttcca caggcggctg cggtcttccg ggcgggtcgc ccattcgaag ccttcgccgg
     2161 cattctgcgc ggcgagctcc tgcaccagtt cggcttgctc tttgacaccg gcttcggtgc
     2221 catggaattc gaaaaagagc gtgggcgcct cacgcaacgt cagattcgaa tgacgattga
     2281 tcgagcggat cgcgagcgag tcgacgaact cgacacgcgc gatcggcacg cccatctgga
     2341 tcgtttcgat gaccgcgcgc acggcgtcgc ccatcgacgg gaaggtgcag accgcagccg
     2401 agaccgcttc gggctgcgga tacagacgca cggtgatctc ggtaatcacg ccgagcgtgc
     2461 cctccgagcc gacgaacaga cgcgtgaggt cgtaacccgc cgacgacttg cgcgcccgcg
     2521 tgcccgtttt gatcatgcgg ccatcggcga gaaccaccgt cagcccgagg acgttttcgc
     2581 gcattgtgcc gtaacgcacg gcattggtgc ccgaggcgcg cgtagccgac atgccgccga
     2641 tgctcgcgtc agcacccggg tcgatcggga aaaacaggcc ggtgtcgcgc agcgcttcgt
     2701 tcaactgctt gcgcgagatg ccgggttcga cggtgacggt caggtcttct gcattgatcg
     2761 acaacacgcg gttcatttcc gacagatcga tcgacacacc accttgcacc gccagcagat
     2821 gcccttcgag cgacgagccg ttgccatagg ggatgatcgg cacgttgtat tggccgcata
     2881 gtttgacgat ggtttgcacg tcttccgtat tgcgtgcgaa tacgacggcg tcgggaagtt
     2941 gggggtcgaa cggcgattcg tcgcggccat gatgggcacg cacggcttct gccgtggaca
     3001 cgcgttcgcc aaaagcggcc ttgagcgcag tcagcaactc ggcgggaaac ggtcggcgca
     3061 gcggggccgg cggcacggga tggttcacgc atgtctcctt gtaatggggc ggaactgttt
     3121 tacagcatgt gcttcatttt acgccgatcg c
//
LOCUS       DATASH010000519         8341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3643609,
            whole genome shotgun sequence.
ACCESSION   DATASH010000519 DATASH010000000
VERSION     DATASH010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 8341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8341
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3643609"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1014
                     /locus_tag="VHV99_14310"
     CDS             <1..1014
                     /locus_tag="VHV99_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX3636630.1"
                     /translation="RTDVLIVGAGPVGLFAAFEAGVIGLSCQIVDALGKVGGQCTELY
                     PDKPIYDIPALPSCTARELVERLMAQCKPFDVPIHLEQRVESVEQRDDGRWTVRTDRG
                     LVFDVAALLLAAGNGAFVPQKLTLAEAVPLESRHVHYSVQRLADFAGKTVVVAGGGDS
                     ALDWALALRQVARRVTLVHRRNGFSAADSSVELMRRAVAAGEMDFVVGAIAGLNVEGD
                     ALKSIALRHIEGETQLDTEQLVVLYGLVADLGPIARWNLSIHGGRVDVDTSNYESSRP
                     GIFAVGDIANYPNKQKLILSGFHEASLALRKAYAYAFPEKKRVHVHSSYDAKLAEKVG
                     AAG"
     gene            complement(1113..1349)
                     /locus_tag="VHV99_14315"
     CDS             complement(1113..1349)
                     /locus_tag="VHV99_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636631.1"
                     /translation="MKRALTTLALTAALALTHTAAQAEGCTKGAAVGGVTGHVAGDHA
                     VAGAAAGCAIGHHEAKKKDKAASAAAAASQPAGK"
     gene            1730..3703
                     /locus_tag="VHV99_14320"
     CDS             1730..3703
                     /locus_tag="VHV99_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX3636632.1"
                     /translation="MNELDVHSARENGEAASVPPSLSADAPVKIDYWALVQSIQDYAI
                     FLLDPSGRIVSWNGGARNLTGYAAHEIIGEHFSRFYTAEAVARDWPAYELQQAALTGR
                     FEDEGWRVRNDGTTFWSNVVITAVRDEAGVLTGFAKITRDLTAQRDYVEALRQSEERF
                     RLLVDSVKDYAIFMLDPDGYVVSWNAGAARIKGYTRDEIVGRHFSSFYTREEAAAGKP
                     ARELAIARQIGRVEDEGWRVRKDGTTFWANVKITAVYDETNRLRGFAKVTRDLTERRE
                     REELERSGERMRKFLATLAHELRNPLAPVRNAIGVMQLEAGMSPTLARSRDLIDRQVT
                     HLTRLVDDLLDVGRIMSDKVELRLARVDLAEVVARAIEAARPFTDSREQSVAPHMPAE
                     PLIVEGDTTRLVQVLQNLLHNASKFSPSGGRIEIFGRIDHHMAVLEVRDPGCGIPVRA
                     LDSIFELFAQEKHGQSLGEGGLGIGLTLCKSLVELHGGSIIATSEGEGCGSAFTLSLP
                     FAAALPDEQTEQDGAAGSTVAPLRILLVDDNRDSADSLAMLLELKGHEVRIAYDGPQA
                     LDVAPRLVPDLAVIDLAMPKMDGYATLAALRAMPALAHTLFAAMTGFGHASDRERTQA
                     AGFHAHLVKPVELALFEALLERAEARRRERAYR"
     gene            3888..5072
                     /locus_tag="VHV99_14325"
                     /pseudo
     CDS             3888..5072
                     /locus_tag="VHV99_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433237.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
     gene            5097..5702
                     /locus_tag="VHV99_14330"
     CDS             5097..5702
                     /locus_tag="VHV99_14330"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018417767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3636633.1"
                     /translation="MSSPARYFDELYKSSDDPWNLREGWYERRKRSLTLALLPRPRYR
                     NAFEPGCANGELTAELAARCDALLAADLHETAVRLARERVAGLPQARVEQRTVPHQWP
                     VDAGPFDLIVISEFAYYLDSADLETLAARIAASLTTDGTLLACHWRRPFAEAIESADT
                     AHALFDARCGLSRLAHHDEADLLIDVWSRDTRSVAQREGLL"
     gene            5699..6352
                     /locus_tag="VHV99_14335"
     CDS             5699..6352
                     /locus_tag="VHV99_14335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3636634.1"
                     /translation="MIGVIVPAHNEEALLAPCLAALIEASRHADLAAETVRIVVVLDA
                     CSDFSGAIARAYGVETLTLKARNVGIARATGADLLLADGARWLAFTDADSRVSPGWLV
                     AQLSLDADAVCGSIAVDDWTAHPHSVREYFRKTYVDADGHRHVHGANLGVSAEAYRLA
                     GGFPPLTCSEDVALVDRLIATGARIAWSAAPRVITSARTATRARGGFGDTLVTWATG"
     gene            complement(6398..7795)
                     /locus_tag="VHV99_14340"
     CDS             complement(6398..7795)
                     /locus_tag="VHV99_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3636635.1"
                     /translation="MTVVQKALIAPLIVACAMFMESVDANVIVTALPAMARDFGRDPV
                     TLKIAVTSYVLGLGVFIPVCGWLADRFGARTVFRTAIGIFVTGSLLCAASNSLATFTL
                     ARFVQGVGGAMMVPVGRIIIFRVVEKADFIRAMNYLSVPAMLGPAAGPLLGGFITTYL
                     HWRLIFFINVPIGILGIYLTNRHIANTREPDPGPLDWIGFFLSAAGAVLLLLGLSLVG
                     GELISNQDAFGMCASGAVLLAGYVAYAQRVELPLLDLRFFKVPTFQASVLGGSLFRIG
                     LGALPFLLPLMLQEGHGMSAFESGLITCGSAFGGMFMRTVAARVLHRFGFRSVLVVNA
                     ALSGISIAACGLFFPGTPTWIIWFVVLLGGFFPALQFTSLNSLTYAEIANRDVGRATS
                     LGSVVQQMSLGLGVTVGGIVLQISRLLQGHSGIMWSDFWPAFLVVGLCSFASIPVTLR
                     MPHRAGEEISRGGRG"
     gene            8032..8187
                     /locus_tag="VHV99_14345"
     CDS             8032..8187
                     /locus_tag="VHV99_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006053307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636636.1"
                     /translation="MNTASNAATARTAAPAANNTWLGKLRALALHALNLHLQNCAVLA
                     EAHRRPQ"
     gene            complement(8211..>8341)
                     /locus_tag="VHV99_14350"
     CDS             complement(8211..>8341)
                     /locus_tag="VHV99_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aromatic compound catabolic protein"
                     /protein_id="HEX3636637.1"
                     /translation="AEGKVIHCGRQLATAEARLVGPDGTLYAHATTTCLVFEIPGR"
BASE COUNT         1496 a         2621 c         2767 g         1457 t
ORIGIN      
        1 cgcaccgatg tcctgatcgt cggcgcgggc cccgtgggcc tctttgccgc tttcgaggcc
       61 ggggtgatcg gtctgtcgtg ccagattgtc gatgcactcg gcaaggtagg cgggcagtgc
      121 acggagctct atccggacaa gccgatctac gacattcccg cgcttccgtc gtgtaccgcg
      181 cgcgagttag tcgagcgttt gatggcgcaa tgcaagccgt tcgacgtgcc gatccatctc
      241 gagcagcggg tcgagtcggt cgagcagcgc gacgatggac gctggaccgt gcgtaccgac
      301 cgtgggctcg tattcgatgt ggcggcgctt ctgcttgccg ccggtaatgg cgcattcgtg
      361 ccgcaaaaac tgacgcttgc cgaagccgtg ccgctggaat cgcggcacgt acactacagc
      421 gtgcaacgtc tcgccgattt cgccggcaag acggtggtgg tggccggcgg cggcgattcc
      481 gcgctcgact gggcgctggc cttgcgccag gtcgcgcggc gcgtgacgct ggtgcatcgt
      541 cgcaacgggt tcagtgccgc ggattcgagc gtcgagttga tgcggcgcgc ggtcgcggcg
      601 ggggagatgg actttgtcgt cggcgcgatt gcgggactca acgtcgaggg tgatgcattg
      661 aagtcgattg cgttgcggca tatcgaaggc gaaacgcagc tcgacacgga gcagcttgtc
      721 gtgctgtatg gactggtcgc cgatctggga ccgatcgcgc ggtggaatct gtcgattcac
      781 ggcggtcgcg tggacgtgga tacgtccaac tacgagagct cacggcccgg catttttgcg
      841 gtcggcgata ttgccaacta tcccaacaag cagaagctga ttctgtccgg ttttcatgaa
      901 gcgtcgttgg cgctgcgtaa agcgtacgcg tacgcctttc ccgagaagaa gcgggtgcac
      961 gtccactcca gctacgacgc gaagctggca gaaaaggttg gggcggcggg ctgaggggtg
     1021 aggggctgac gcgggcatcc gccgtgcgcg ttgcgaattg atcgagcccg gcggtgatcg
     1081 atagatcacc gccgggctct ttttacatgc gcttatttgc ctgcgggttg gctggcggct
     1141 gcggccgcgc tcgctgcttt gtctttcttt tttgcctcgt gatggccgat tgcgcagccc
     1201 gcagccgcgc cggccacggc atgatcgccg gcgacgtgcc cggtgacacc gcctaccgcg
     1261 gcgcctttag tgcagccttc ggcttgcgcg gcggtgtgcg tcagagcgag tgccgcggtg
     1321 agggctaagg tggttaatgc tcgtttcaag tcaggctcct tggtttcaga tagaggtgct
     1381 ttgacgagac agcaagcgag aggcccgatc gaggtagcgc cccgctcata cacaaaggtc
     1441 tccaagggat ccagacggag agcgccctcc cgccaactat ctcctgacag gcctgttttt
     1501 tgctgcgccc caaaattggc aagccccggc gcgaggtcga agccccgcgg cggcgcaaac
     1561 aaaaacgaag ggagcaatcg gagcatgcaa cgagcggcgc gcacaaatag cgccgcgcgc
     1621 ctggcggcat gggcgtctgt ttccatcgcc gaagcggtaa tgcagggcac tggcgaatcg
     1681 tgaagcgagc cggtgtatgc tttgaacaga cccatcgata cttcttacca tgaacgagct
     1741 tgacgtccat tcagcccgcg aaaatggcga ggccgccagc gtgccgccct cgctcagtgc
     1801 cgacgcgcct gtcaaaattg attactgggc gctggtccag tcgatccagg actacgcgat
     1861 ttttctgctg gatccaagcg ggcgtatcgt cagctggaat ggcggtgcgc ggaacctgac
     1921 gggctacgcg gcgcacgaaa tcatcggcga gcacttctcg cgtttctaca cggccgaagc
     1981 cgtggcgcgc gattggcccg cttacgagtt gcagcaagcc gcgctcaccg gccgcttcga
     2041 ggatgaaggc tggcgggttc gcaatgacgg cacgaccttc tggtcgaatg tggtcatcac
     2101 ggcagtgcgc gacgaggcgg gtgtgttgac gggctttgcc aagatcactc gcgatctgac
     2161 cgcgcagcgc gattatgtgg aagcgttgcg gcaaagcgag gagcgtttcc ggttgctggt
     2221 cgacagcgtg aaagactacg cgattttcat gctcgatccg gacggttatg tggtgagctg
     2281 gaatgcgggt gctgcgcgca tcaaaggcta tacgcgcgac gaaatcgtgg gccggcattt
     2341 ttcgagtttc tatacgcgcg aagaagcggc cgccggcaag cccgcgcgcg aactggcgat
     2401 cgccaggcag atcggccggg tcgaagacga aggctggcgc gtgcgcaagg atggcacgac
     2461 cttctgggcc aacgtcaaaa tcactgccgt gtacgacgaa acgaaccgct tacgtggctt
     2521 cgccaaggtc acgcgcgatc tgaccgagcg ccgtgagcgc gaagagctgg agcgctccgg
     2581 cgagcgtatg cgcaagtttc tcgcgaccct tgcccacgag ttgcgaaatc cgcttgcgcc
     2641 ggtgcgcaac gcgatcggcg tgatgcaact cgaagcgggc atgagcccca cgttggcgcg
     2701 ctcgcgcgat ctgatcgacc gccaggtcac gcacctcacg cgtctcgtcg acgatctgct
     2761 cgacgtcggc cgcatcatgt cggacaaggt cgaactgcgc ctggcgcgcg tcgatctggc
     2821 cgaagtggtg gcgcgcgcga tcgaagcggc gcggccgttt accgattcgc gagagcagag
     2881 cgtggcgccg catatgccgg ccgagccgct gatcgtcgag ggcgacacga cgcggctcgt
     2941 gcaggtgctg caaaacctgt tgcataacgc gtcgaagttt tcgccgtccg gcggccgcat
     3001 cgagatcttc ggccgcatcg atcatcacat ggctgtgctg gaggtgcgcg accccggctg
     3061 cggcattccc gtacgtgcgc tcgattcgat cttcgagttg ttcgcgcagg aaaaacatgg
     3121 gcaaagcctc ggcgaaggcg gtctcggtat cggcctcacg ttgtgcaaat cgctggtcga
     3181 attgcatggc ggcagcatta tcgcgaccag cgagggagag gggtgcggga gtgcgttcac
     3241 gctcagtttg ccgtttgccg cggcgctccc cgacgagcag actgaacagg acggtgcggc
     3301 gggaagcacg gtggcgccgc tgcgtattct gctggtggac gacaaccgcg actccgccga
     3361 cagcctcgcc atgctgctcg aactcaaagg ccacgaagtc cgcattgcct acgacggccc
     3421 gcaggcgctc gatgtcgcgc cccgcctcgt gccggacctt gcagtgatcg acctcgcgat
     3481 gcccaaaatg gacggctacg cgacgctcgc cgcattgcga gcgatgcccg cgctggctca
     3541 cacgctgttc gccgcgatga ccggcttcgg tcacgccagc gatcgcgaac gcacgcaagc
     3601 ggccggtttc cacgcccatc tcgtcaagcc ggtggaactc gcgctgttcg aagcgttgct
     3661 ggagcgggcg gaagcgcggc gccgggagcg ggcttaccgc tgatgtccga gtcgcgcttc
     3721 cttcatacag aagcgctcca agccgtcttc aataggccat aacccctgaa atgttgcggc
     3781 gtgtcccagg tattcgagac ctgcggcacg ccgcttgctg ttggtctgca agcgctgcgg
     3841 ctatcaaaag tccgcgcgta tagatcggac agcttcaggg aggcacgatg cacgatacgc
     3901 gcgatcgtgc ggccggcgtg agtgcgcggc cgccggaagg aacgggcagt acccgcactc
     3961 gcgatccatc cgcggcactc gccgcgcagc aggccagggt ttctgccggg tcgcttgaag
     4021 attttctgcg cggcacgcat ttcgatgcaa gtgaccccgc cgcgctgggc gatgtgttgg
     4081 gcgcactgat tgaacgcggc tacgaccgtt tgcccttcgt gccattgccc gggcacgggc
     4141 agacgctggt gcgctggcgt gccctggccg cggttgcggc gtgcgatctc ggtctcgtca
     4201 aactgttcga aggacatacc gatgcgctgg cgattctcgc cgagctgcgc ggccccacgc
     4261 cgcccgtggg gagccgctgg ggtgtgtggg ccgcggagcc gcctgatgcg cgcgtgcagg
     4321 ccagcaaacg cgcgactcgt accgacgcga cgcccacgga cgatcccgag cacctctatc
     4381 tgagcggcac aaaagcctgg tgttcaggcg cggccgtcgt cagtcacgcg ctcgtcaccg
     4441 catggctcga cgacgaaccc gtgcttgccg ccgtggcgat ggatcacgcg tcgatttcgc
     4501 tcgacgcgtc gaagtggcaa gcagtcggca tgcaggcatc cgccagcgcc gacgtcacct
     4561 tcgatcagac gccggccaca cgcgtgggcg cggctcatgc ctaggtgcgc cggccgggct
     4621 tctggcatgg cggcgccggc attgccgcgt gctggtacgg cgccgccgcg caaatcggcc
     4681 ggacgttgcg tgatgcatcc atgcaacgtg ccgatgcgca tcggttcgcg cacctcggcg
     4741 cggtcgaggt cgctttagcg ggcgccgccg cggtgctgcg cgaaaccgcc gctcatatcg
     4801 acgcgaatcc acacgccgac gcacagcgtg aagccatgcg cgcccgtctc atcgttgaag
     4861 aagccgcaac ggcggtgatg aatcacgcga cgcgcacact cggcgccggc ccgctctgcc
     4921 gcgatgcgcg ctttgcccgg gcgctggcgg atctgccggt gttcttgcgg caaagccatg
     4981 ccgaacgcga tcttgccgcg ctcggcgaat tgaccgtcaa ggcaaaggac gaccgcaacg
     5041 ccgctgccgg aggtgcgcct tggacgctct gacacgattc atggagggcc gctgacatgt
     5101 cctccccagc gaggtacttc gatgaactct acaaaagcag cgacgatccg tggaatctgc
     5161 gtgaaggctg gtacgaacgc cgcaagcgtt cgctcacgct tgcgctgctg ccgcgtccgc
     5221 gctatcgcaa cgcgttcgag ccaggctgcg cgaacggcga actgacggcc gaactggcgg
     5281 cccgctgtga cgcgctgctc gccgccgatc tgcacgaaac agcggtacgt cttgcgcgcg
     5341 aacgggttgc cgggctgccg caggctcgcg tcgaacagcg cacggtgccg caccaatggc
     5401 cggttgacgc gggaccgttc gatctgatcg tgatcagcga attcgcctat tacctcgatt
     5461 cagccgacct cgaaacgctg gccgcgcgaa tcgccgcctc gctcacgacg gacggcacgc
     5521 tgctcgcctg ccactggcgt cgtccgtttg ccgaagcgat cgaatccgcc gacaccgccc
     5581 acgccttgtt cgacgcgcgc tgcggtctgt cgcgtctcgc gcatcacgac gaagccgatc
     5641 tgctgatcga cgtatggtcg cgcgatacgc ggtcggtggc acaacgcgag gggctcctat
     5701 gatcggcgtg atcgttccgg cgcataacga agaggcgttg ctggcgcctt gtctggccgc
     5761 gttgatcgaa gcctcgcggc atgctgatct agccgctgaa acggtgcgga tcgtcgtggt
     5821 gctcgacgcc tgcagcgatt tttccggggc gatcgcgcgt gcttacggtg tcgagacatt
     5881 gacgctgaag gcgcgcaatg tcggcatcgc gcgtgcgacg ggtgccgatt tgctgctggc
     5941 ggacggcgcc cgctggcttg ccttcaccga tgccgacagc cgcgtgtcgc cgggctggct
     6001 ggtcgcccag ttgtcgctgg acgcggacgc ggtgtgcggc tcgattgccg tcgacgattg
     6061 gaccgctcat ccgcacagcg tgcgcgaata ttttcgcaag acctatgtgg acgcggatgg
     6121 ccaccgtcat gtgcacggcg cgaatctcgg cgtctccgcc gaggcgtatc gactagccgg
     6181 cggctttcca ccgctcacgt gcagcgaaga cgttgcgctc gtcgaccgcc tgatcgcgac
     6241 cggtgcccgc attgcatgga gtgccgcgcc gcgcgtgatc acaagcgcgc gcacggcgac
     6301 ccgcgctcgc ggcggtttcg gcgacacgct tgtcacatgg gcgacgggct gaggcgggcg
     6361 gtggtggaag cccgctcgag gttgacaagg cgagccttta tccccgaccg cctcgcgaga
     6421 tttcttcacc ggcgcgatgc ggcatacgca acgtgaccgg aatcgacgca aacgaacaca
     6481 agcccaccac cagaaacgcg ggccagaaat ccgaccacat gatgcccgaa tggccttgca
     6541 gcaggcgcga aatctgcagc acgataccgc ccaccgtcac gcccaggccg agcgacatct
     6601 gttgcacgac gctgccgaga ctcgttgcgc gccccacgtc gcggttggcg atttccgcgt
     6661 aagtcagcga gttgagactc gtgaattgca gcgcggggaa gaagccaccg agcaacacga
     6721 cgaaccagat gatccaggtc ggcgtgccag ggaaaaacag tccgcacgcc gcgatcgaga
     6781 tccccgacag cgccgcgttc accaccagca ccgaacggaa gccgaagcga tgcagcacgc
     6841 gcgcggcgac cgtgcgcatg aacatgccgc cgaacgcgga cccgcacgtg atcaggccgg
     6901 actcgaacgc gctcatgccg tgtccctcct gcaacatcag cggcagcaga aacggcaacg
     6961 cgccgagacc gatacgaaac agcgagccgc cgagcacgct cgcctgaaac gtcggtactt
     7021 tgaagaagcg cagatcgagc agcgggagtt cgacgcgttg ggcgtacgcg acatagcccg
     7081 cgagcagcac ggcgccgctc gcgcacatgc cgaacgcgtc ctgattcgaa atgagctcac
     7141 cgccgaccag cgacaatccg agcagcagca acacggcgcc ggccgccgac agaaaaaaac
     7201 cgatccagtc gagcggcccc gggtcgggct cgcgtgtgtt ggcgatgtgc cggttggtca
     7261 gatagatacc gagaatgcca atcggcacgt tgatgaaaaa gatcagacgc caatgcagat
     7321 aggtggtgat gaagccgccg agcagcggtc cggcggccgg tccgagcatc gccgggacgc
     7381 tcaggtagtt catcgcgcgg atgaagtccg ctttttcgac cacccggaag atgatgatcc
     7441 gcccgaccgg caccatcatc gcgccgccca cgccttgcac gaaacgggcg agggtgaagg
     7501 tagccagcga attcgacgcg gcgcacaaga gcgagccggt cacgaagatg ccgatcgccg
     7561 tgcggaaaac cgtgcgtgcg ccgaagcggt ccgcgagcca gccgcataca gggataaaga
     7621 cgccgagacc cagcacgtag ctcgtcacgg cgattttcag cgtgaccgga tcgcgcccga
     7681 aatcgcgcgc catggcgggc aaggcggtga cgatcacgtt ggcgtcgacg ctttccatga
     7741 acatcgcgca ggcgacgatg aggggtgcaa tcagggcttt ctggacgacg gtcatgaggg
     7801 gcggggcagt ccgcggcgaa tggtcgcagg taaagggggc attattgcac ctgtacggtg
     7861 cggatggtta atagtgcagc gcgcgatgcc gccccttttt gagattttgt aggttgcgcc
     7921 tgtggccgtg gcacaacatt tcggtgggtt attgatattt gttgcagtgc ggcgtaggag
     7981 ggctataatc gggttattgc ctaggtataa accctataaa aggaatccac catgaacacc
     8041 gcttccaacg ccgctaccgc ccgcaccgca gcacccgccg ctaacaacac gtggctgggc
     8101 aagctccgtg cgctggcttt gcacgcgctg aacctgcatc tgcaaaactg cgcggtgctt
     8161 gctgaagcgc accgccgccc gcagtaagcg ttccgtatcc accgtatcct ttagcggccc
     8221 ggaatctcga acaccagaca ggtcgtcgtg gcatgcgcgt agagcgtgcc gtcggggccg
     8281 acaaggcgag cttccgccgt cgccagctgc cgtccgcagt ggatcacctt gccttcggcg
     8341 c
//
LOCUS       DATASH010000520         5653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4634855,
            whole genome shotgun sequence.
ACCESSION   DATASH010000520 DATASH010000000
VERSION     DATASH010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5653
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4634855"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4341
                     /locus_tag="VHV99_14355"
     CDS             <1..4341
                     /locus_tag="VHV99_14355"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HEX3636638.1"
                     /translation="GDAAKLDALRDEIAKVLRDVRAAVEDWPKIVELARLTIKGMKTG
                     EAGPEGVEARAFLEWMVADHFTFLGQRDYELVQHDTGFGLRALPGSGLGILRDALRPE
                     GAAEVTPLPPAAADIISGSSPIFLTKANSRATVHRPGYLDYVGIKLTGADGKVTGERR
                     FIGLYTSTAYLVSAAEIPIARRKCANIVRRAGFLPKGHLAKSLVTVLETYPRDELFQA
                     DESQLYDIALGVLRLQEHQRTRLFVRRDRFDRFVSCLVFVPRDKYNTDLRQRIANLLA
                     DAFNGESVEFTPLLSESTLARIHFVVHAKPGGMPTVDTRELEARLVHVTRRWQDDLAD
                     ALLDAFGEEQGNRLLQHYADSFPPGYRDDYPARTAVRDIELIERVQGSERLAMNLYRP
                     IEAGPRAFRFKVYRAGSPIALSRSLPMLEHLGVRVDEERPYLIEAPDATPAWIHDFGL
                     VLADDAEFDIERVKDLFEEAFEQVWTGGIESDDFNRLVLRAQLNAREVTILRAYAKYL
                     RQVGSTFSDAYIERAVTGNPAIARMLVELFIARFDPVLGETREARVDGWLKTIDSALD
                     QVPNLDEDRILRQFLGVIKATQRTNYYRFDADGHPKSYLSFKFNPAQVPGLPEPKPMF
                     EIWVYSPRVEGVHLRGGRVARGGLRWSDRREDFRTEVLGLMKAQMVKNVVIVPVGSKG
                     GFVVKNPPPQTERDAWMREGVACYQTFLRGLLDLTDNLAGTTVVPPPDVVRHDPDDPY
                     LVVAADKGTATFSDYANAISQEYGFWLDDAFASGGSVGYDHKKMAITARGAWESVKRH
                     FREMGVDTQTTDFTVVGVGDMSGDVFGNGMLLSPHIKLVAAFDHRHIFLDPNPDPAGS
                     LAERGRLFVLDRSSWADYDPSLISAGGGVFPRSAKTIPLSPAVQSVLGISAPALAPAE
                     LMRAILQAPVDLLYNGGIGTYVKASRETHLQVGDRANDAIRVNGADLHCKVVAEGGNL
                     GLTQLGRIEFAQRGGRINTDAIDNSAGVDCSDHEVNIKILLGLVVADGEMTGKQRNVL
                     LAEMTDEVGLLVLQDNYYQTQALSIAGRYGVELLDAEARLMRYLERAGRLNRVIEFLP
                     TDEEVAERQAAKQGLTTPERAVLLAYSKMWLYDALLESSMPEDPLVGDLLIEYFPKPL
                     RQRFSEPMQRHPLRREILATHLTNALVNRVGCEFVHRLMEETDAKPGDIVRACIMARD
                     VFDLDDVWRSIDALDNRVADDVQARMFVEVARLVERSALWFLRHLQSGAISDGDVAGL
                     LARCRDAAQRLAAQWPALLPAADLEALSERQRVLVDAGVDSELAVRIASGEISAALLD
                     IAEVASTCGRSLELAAGVYFALGTLLNYGWISERAAALPAPTHWDMLARATALAELAR
                     LKRALTTSALAGADDASTPDALVQSWREKRAAQLERYARLLADLRATGGASLSMLLVI
                     VREMAALERA"
     gene            4486..5391
                     /locus_tag="VHV99_14360"
     CDS             4486..5391
                     /locus_tag="VHV99_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX3636639.1"
                     /translation="MKEESPKAIFYALAANLGIAICKFAAATFTGSGSMFAEAIHSSA
                     DCGNQLLLLFGLREARKPASPLHPMGGGREINFYSLLVALLLFFVGGAFSVYEGVHRL
                     FAREPLQYAYVALIVLGVSVVLEALSLWGAVKEIRKTHPDKSMWRWFRETRESELLVV
                     AGEDIAALAGLAMAFVAVLLTMVTGNPVYDALGSIGVGVLLMVIAWLVAREVKSMIVG
                     ESASPEVRRAIEAHLRARTEIRSIINLITLQWGRHVVVAVQAEMVDYGSGRAMVDAIN
                     MIEADLQAAFPQVRWVFFEPDVPRV"
BASE COUNT          981 a         1717 c         1850 g         1105 t
ORIGIN      
        1 ggcgacgcgg cgaagctcga cgccttgcgt gacgagatcg ccaaggtgct gcgcgacgtg
       61 cgcgccgcgg tggaagactg gccgaagatc gtcgaactcg cgcgccttac catcaaaggc
      121 atgaagacgg gcgaggcggg ccccgaaggt gtcgaagccc gcgcgtttct cgaatggatg
      181 gtggccgatc atttcacctt tctaggtcag cgcgactatg aactggtgca gcacgacacc
      241 ggctttggct tgcgcgcgct gcctggctcc ggcctcggca ttctgcgtga cgcgctgcgt
      301 cccgaaggcg ccgccgaagt cacgccgttg ccgccggcgg ccgccgatat catctccggc
      361 tcgtcaccga tcttcctgac caaggccaac tcgcgcgcga cggtgcatcg gccgggctat
      421 ctggattacg tcggcatcaa gctgaccggt gcggacggca aggtgaccgg cgagcggcgt
      481 ttcatcggcc tgtacacgtc gaccgcatac ctggtctcgg ccgcggagat cccgattgcg
      541 cggcgcaaat gcgcgaacat cgtgcggcgg gccggtttcc tgccgaaggg ccatctcgcc
      601 aaatcactgg tgacggtgct cgaaacctat ccgcgcgacg aactctttca ggccgacgaa
      661 agccagctct acgatatcgc gctcggtgtg ctgcgtttgc aggagcacca gcgcacgcgc
      721 ctgttcgtgc gacgtgatcg tttcgaccgt ttcgtgtcgt gtctcgtgtt cgtcccgcgc
      781 gacaagtaca acaccgatct gcggcagcgt attgccaacc tgctggccga tgcattcaac
      841 ggggaaagcg tcgagttcac gccgctgttg tcggaatcga ccctcgcgcg tattcacttc
      901 gtcgtgcatg cgaagccggg tggcatgccc actgtcgata cacgtgaact cgaagcgcgc
      961 ctcgtgcatg tcacgcgccg ctggcaggac gatcttgccg acgcattact cgacgcattc
     1021 ggcgaggagc agggcaaccg tctcctgcaa cactatgcgg attcgtttcc cccgggctat
     1081 cgcgacgatt atccggctcg cacggcagtg cgcgatatcg agctgatcga gcgcgtgcag
     1141 ggcagcgagc ggctcgccat gaacctgtac cgcccgatcg aagccgggcc gcgcgcgttt
     1201 cgcttcaagg tttatcgcgc ggggtcgcca attgcgttgt cgcgcagctt gccgatgctc
     1261 gaacatctcg gcgtgcgtgt cgacgaggag cggccttatc tgatcgaagc gcccgacgcc
     1321 acgcctgcgt ggattcacga cttcggtctc gtactcgcgg acgacgcgga gttcgatatc
     1381 gagcgtgtga aggatctatt cgaagaagcg ttcgaacagg tgtggaccgg cggaatcgaa
     1441 agcgacgatt tcaaccgcct tgtgttgcgc gcgcaactga atgcgcgcga ggtgacgatt
     1501 ctgcgtgctt atgccaagta cttgcggcaa gtaggctcga ccttcagcga cgcgtatatc
     1561 gaacgtgcgg tgaccggcaa tccggcgatt gcccgcatgc tcgtggaact cttcatcgcg
     1621 cgctttgatc cggtgctcgg cgagacccgc gaggcgcgcg tcgacggctg gttgaagacg
     1681 atcgacagcg cgctcgatca ggtgccgaat ctcgatgaag accggatact ccggcaattc
     1741 ctcggtgtca tcaaggccac gcagcgcacc aactactatc gtttcgacgc tgacggtcat
     1801 ccgaaatcgt atctgtcatt caagttcaat cccgcgcaag tgcccggttt gcctgaaccg
     1861 aaaccgatgt tcgaaatctg ggtctattcg ccgcgtgtgg aaggcgtgca tttgcgcggc
     1921 ggtcgtgtcg cgcgcggcgg actgcgctgg tcggatcgcc gggaggactt ccgcacggaa
     1981 gtgctcgggc tgatgaaggc gcagatggtg aagaacgtgg tgatcgtgcc ggtcggctcc
     2041 aagggcggct tcgtcgtgaa gaatccgccg ccgcagacgg aacgcgacgc gtggatgcgc
     2101 gaaggcgtcg cctgctatca gacgttcctg cgcggcctgc tcgatctgac cgacaatctc
     2161 gcgggcacga cggtggtgcc gccgccggat gtggtgcggc atgaccccga cgatccctat
     2221 ctggtcgtcg ccgccgacaa gggcaccgcc accttctccg actacgcgaa cgcgatctcg
     2281 caggaatatg gtttctggct cgacgacgcc ttcgcgtcgg gcggctcggt cggttatgac
     2341 cacaagaaaa tggcgatcac ggcgcgcggc gcgtgggagt cggtcaagcg gcatttccgt
     2401 gaaatgggcg tcgatacgca aacaaccgat ttcacggtgg tgggcgtcgg cgatatgtcg
     2461 ggcgatgtat ttggcaacgg catgctgcta tcgccgcata tcaaactggt cgccgcgttc
     2521 gatcatcggc atatctttct cgatccgaat cccgatccag cgggaagcct tgccgaacgc
     2581 gggcgtctgt tcgtactgga tcggtcgagt tgggccgact atgatccttc gctgatctcg
     2641 gcgggcggcg gcgtgtttcc gcgctcggcc aagacgattc cgctgtcgcc ggcggtgcag
     2701 tcggtgcttg gcatcagcgc gccggcgctc gcgccggctg aattgatgcg cgcgattctg
     2761 caggcgcccg tcgatctgct ctacaacggc ggcatcggca cctatgtgaa agcgagccgc
     2821 gaaacccatc tgcaagtcgg cgatcgcgcc aacgatgcga tccgtgtcaa tggcgccgat
     2881 ctgcactgca aggtcgtggc ggaaggcggc aatctcggtc tcacgcaact tgggcgcatt
     2941 gaattcgcgc agcgcggcgg ccgcatcaat accgatgcga tcgacaactc cgccggtgtc
     3001 gactgttcgg accacgaggt caacatcaag attctgctgg gcctggttgt cgccgacggt
     3061 gaaatgaccg ggaagcagcg caatgtattg ctcgcggaaa tgaccgatga agtcggcttg
     3121 ctcgtgttgc aggacaacta ttaccagact caggcgctct cgatcgcggg ccgctacggc
     3181 gtcgaattgc tcgatgccga agcgcgtctg atgcgttacc tcgaacgcgc gggccggctg
     3241 aaccgcgtga tcgagttctt gccgaccgat gaggaagtcg ccgagcgcca ggccgcgaaa
     3301 caagggctga ccacgcccga gcgcgcggtg ctgcttgcct acagcaaaat gtggctgtac
     3361 gacgcgctgc tcgaatcgtc gatgccggaa gacccgctcg tcggcgacct gctgatcgaa
     3421 tactttccga agccgttgcg gcagcgcttt agcgaaccga tgcaacgcca tccgctgcgg
     3481 cgcgagattc ttgccacgca cttgaccaat gcgctggtga atcgcgtggg ctgcgagttc
     3541 gtgcatcgcc tgatggaaga gaccgatgcg aagcccggcg acatcgtgcg ggcctgcatc
     3601 atggcgcgcg acgtgttcga tctcgacgac gtgtggcgca gtatcgacgc gctcgacaac
     3661 cgtgtcgccg acgatgtgca ggcgcgtatg tttgtcgaag tggcgcggct cgtcgaacgc
     3721 tcggcgctgt ggttcctgcg gcacttgcaa tcgggcgcga tcagcgacgg tgatgtcgcc
     3781 ggcttgctcg cacgttgccg tgacgcggcg caacgcctcg ctgcgcaatg gcccgcgctg
     3841 ctgcccgccg ccgatctcga ggcgctgtcc gagcggcaac gggtgctggt ggacgcgggc
     3901 gtcgacagcg aactggcggt gcggatcgcc agcggcgaaa tttcggcggc cttgctcgac
     3961 atcgccgagg tggcatcgac ctgcgggcgc agtctcgaac tcgcggcggg ggtctacttc
     4021 gcgctcggta cgctgctgaa ctacggctgg atcagcgagc gcgcggccgc actgccggcg
     4081 cccacgcact gggacatgct ggcgcgcgcc acggcgctgg ccgaacttgc ccgcctgaag
     4141 cgcgcgttga ccacgagcgc gctggccggc gccgatgatg cgtcgacgcc ggatgcattg
     4201 gtccagtcgt ggcgtgagaa gcgcgccgcg caactggagc gatatgcgcg cctgctcgcc
     4261 gacctgcggg ccacgggcgg tgcgagcctg tcgatgctgc tggtgatcgt gcgggagatg
     4321 gctgcgctgg agcgcgcata acacgacgcg aggccggcga gggggaccgt gcagaatacg
     4381 gtgcaaaaca gcgccggggt tggacgcatg ccgtccggcg tgtccataat acgaactatt
     4441 gccaaccgaa agcgttctgc aaactcacgg atcccctcca cgacgatgaa agaagaatcg
     4501 ccgaaagcca ttttctatgc gctcgccgcc aacctcggca tcgccatctg caagttcgct
     4561 gctgccacct tcaccggctc cggctcgatg ttcgccgaag ccattcactc gagcgccgac
     4621 tgcggcaacc aactgctgct gctattcggc ctgcgcgaag cgcgcaaacc ggccagcccg
     4681 ttgcatccca tgggcggtgg ccgcgagatc aatttctatt cgttgctggt ggcgttgctg
     4741 ctgttctttg tcggcggtgc gttttcggtg tacgagggcg tacatcgtct gtttgcgcgc
     4801 gagccgttgc agtatgcgta tgtggcgctg attgtgcttg gcgtttcagt ggtgctcgaa
     4861 gccctttcgt tgtggggtgc ggtcaaggag atccgcaaga cgcatcccga caaaagcatg
     4921 tggcgttggt ttcgggaaac gcgcgagtcc gaattgctgg tggtggcagg cgaggacatc
     4981 gccgcgttag caggccttgc gatggccttc gtcgccgtcc tgctgaccat ggtgaccggc
     5041 aatcctgttt acgacgcctt gggttccatc ggtgtgggtg tgttgctgat ggtgattgcg
     5101 tggctggtgg cgcgcgaggt gaagtcgatg atcgtcggcg aatcggcgag cccggaagtg
     5161 cgccgcgcga tcgaggcgca tttgcgcgct cgaaccgaaa tccgcagcat catcaatctg
     5221 attacgctgc aatggggacg ccatgtggtc gtggccgtgc aggccgaaat ggtcgattac
     5281 gggagcgggc gggcgatggt cgatgcgatc aacatgatcg aggcggattt gcaggctgcg
     5341 tttccgcagg tgcgctgggt gttcttcgaa ccggatgtgc cgcgcgtcta gaagtacttc
     5401 tccttggccg cccgctctcg cgagcttcgt gtgagtgagc ggcatgccgt gatacgcagt
     5461 gaaaataaaa tcttcgcatc tatacttctt tcggcttgag cgcggccttt tcccaatctg
     5521 catcacaagc aaaaggaaga tcacgatgct gactcgcaca ttcggcgctc tttccgtcat
     5581 cgcactcgcg gcatgcgcag ccttcgcgtc cggacccgcc agcgcggaca acaacgacgc
     5641 ttcctcgcac gag
//
LOCUS       DATASH010000521        11652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_83022,
            whole genome shotgun sequence.
ACCESSION   DATASH010000521 DATASH010000000
VERSION     DATASH010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 11652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11652
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83022"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VHV99_14365"
     CDS             <1..483
                     /locus_tag="VHV99_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEX3636640.1"
                     /translation="LMAVDLRGRFEEPYPFWNASVGAPAARLRDDLELLPQLAENGLC
                     DAAKDISMAGTLGTALMLLECSQVGARIDLDAIPKPADVPLARWVTAFPSFGYLLSVR
                     ESDVERVQARFEARSLACAEIGTVDATRQAVVHRGDDVAVLWDFSGESFIVPADQVPR
                     "
     gene            480..1658
                     /locus_tag="VHV99_14370"
     CDS             480..1658
                     /locus_tag="VHV99_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_0565 family glycosyltransferase"
                     /protein_id="HEX3636641.1"
                     /translation="MSMDALRIALLTHSVNPRGGVVHTLELASALSAQGHDVTVFAPA
                     TNGETLFRTPPCRVVYAPVTDSQAGTVALVDARIRALKRSVRAHGPTAFDVLHAEDSI
                     SGNALAELKEEGVIRGFVRTVHHLDHFDDPRLAAWQARAYRDADQVFCVSDTWTREMS
                     GRYGIAAVTVSNGVDTARYTAQALPADEALRQRLGLGAGPLILAVGGVEARKNTLALL
                     DGFALMRRALPLAQLVIAGGASLLDHDGYTQRFIQCAAQHGLSIGTHESIVVTGPLED
                     AAMPALFRSADVLAMVSVREGFGLVVLEALACGTPVVASAIAPFTEYLDDATCFWATP
                     TDADSIAAALLRVIRGDSAIDFAHAVPALLARFSWDASARRHVDAYRAWLAHQPLAIH
                     "
     gene            1682..1966
                     /locus_tag="VHV99_14375"
     CDS             1682..1966
                     /locus_tag="VHV99_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007731610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_0570 family nitrogen starvation response
                     protein"
                     /protein_id="HEX3636642.1"
                     /translation="MPVMHFRIQWPDGDEANCYSPSQVVSEFFTPGHAYPLDDFVRRS
                     REALNIASERVRQKYGFACSAAMDQLAQIEQQADRFRNRPDATVKVIELL"
     gene            2049..3350
                     /locus_tag="VHV99_14380"
     CDS             2049..3350
                     /locus_tag="VHV99_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007731608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_0569 family flavin-dependent
                     oxidoreductase"
                     /protein_id="HEX3636643.1"
                     /translation="MSNTAHSLSAGTRENRHYSVLVVGGGQAGLSISYYLKRAGVDHL
                     VVEKNTVTHTWRNQRWDAFCLVTPNWQCALPGYPYPGDDPHGFMNKDEIIAYLDGFIA
                     MVDAPVIEHTQVKRVKRLDDGTFAVATSQGDFTADQVVVASGGYHTPIVPRMAERLPA
                     QIVQLQSSEYRNPQALPEGAVMVVGSGQSGAQIAEDLHLAGRKVVLAVGEAPRCARFY
                     RGRDVVDWLADMKYYDMPVEMHPLREGVRDNTNHYVTGRDGGRDIDLRKFAAEGMELY
                     GRLDDLQNGHLQFAPNLTVNLDDADDTYNRINASIDAFIEKNGIDAPPGGVYEPLWQP
                     RHERTTLELAGSGITSIIWCIGFTPDFSWIDAPVFNGRGYPAHTRGITPVEGLYFIGL
                     PWLHTWGSGRFSGIARDAAYVVQAILASVRERTSSCDTVAA"
     gene            3356..4189
                     /locus_tag="VHV99_14385"
     CDS             3356..4189
                     /locus_tag="VHV99_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pnap_2097 family protein"
                     /protein_id="HEX3636644.1"
                     /translation="MQQTTQSYRAGMPQLAYTGLSENWLIKECGHRHWEALAGDAGRR
                     APDFVDDDGSKSYAAFTAIRLTGATLHAIGENDDFELGTGLCRTGPARHYSQHRLLSG
                     NGECAIVSMSSTFVRRHEVRNNRSVTRATFAAMGGPTPTPPADAVDMARRGKQFRAGQ
                     WDERLPFTSADRVGNDWIEFLPCPNNDFNGADFLYFASFQSFVDRAEWQRHRFHEPPV
                     IAGRELFFYGNIDVGDTLGLRVVAEHKDERGLVHWCELRRGSDQQKIADVVTSKHWRQ
                     R"
     gene            4186..6321
                     /gene="scpA"
                     /locus_tag="VHV99_14390"
     CDS             4186..6321
                     /gene="scpA"
                     /locus_tag="VHV99_14390"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013956889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HEX3636645.1"
                     /translation="MNREGDLRGDRGFADESRDAVRHPPNAVPLKALYTESDLEGVSH
                     LGSRPGEAPFVRGPHASMYTQKPWTIRQYAGYADAADSNLAFRTALAEGAQGLSVAFD
                     LPTHRGYDSDDPAAVADVGMAGVAIDSVEDMKRLFRQIPLDRVSVSMTMNGAVLPVLA
                     AFIVAAQESGVHLEALTGTIQNDILKEYMVRNAWIFAPEPSLRIVADVVEYLAERAPR
                     FNAISISGYHFQEAGADAVLELALTLSNARTYVDALVKRGMNVERIGERLSFFFGVGT
                     EFFVEVAKLRAARVVWAGIMSELGARGARAQSLRMHCQTSGWSLTAQHPQNNIVRTTI
                     EAMAAVFGGTQSLHTNAYDEALALPSADASRLARDTQLIMQHEMGLCDVIDPWAGSYM
                     MESITDDIARRVRDLMTQIATQGGVVEAIRSGWVQQRIHEAATRTQARIDAGQRVVVG
                     VNRFRSPDADTPSNCLEIDGRRVRARQVRQLAELKAQRDDVLVSHALAALADGARDGT
                     GNLLALTIECVRARATIGECTRALEAVWPRYSMPSRFSSGVYGEARVIDGAWRDACDR
                     VSALAARLGRLPRVLIAKVGQDGHDRGAKTVAAALADAGFYVSLGALFRSPGEVVDDA
                     IAAKADVVGISSLSGAHLDLVGEVVRTLRVRRPRAAVVVGGIIPDEHARLLKRNGICA
                     VLGPGAAMESIVANFVEAVEVASASAVAR"
     gene            complement(7367..9472)
                     /locus_tag="VHV99_14395"
     CDS             complement(7367..9472)
                     /locus_tag="VHV99_14395"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX3636646.1"
                     /translation="MLGQLAGLERLLTPINDALAVIDGRRDCGDRVRLILLQGCAERR
                     TTFWAWDETIWRKVLGTNQQVFVSAHDVKPRAAGERLALIAVAYLLRCFRDISSLGEV
                     KRVALAEKIFGKDQVSTTLTRVTDVVTAWGYRSPNKPLISMVAELLLINQRPELESLT
                     ADFIGAVRARWRDSNHRSSLYFQLARVLATLGIVSDEPKVRDTVDREFIAAGRIAGIA
                     ADWVAMVERWEATSTLTAVSRRQFRNMILKAGRWLKDRHPEVTGADQWTRELAAEYVS
                     DVDRMRVGDYIWHTAGICNGLGKPITARSKNALLGSMRCFFTDAQEWEWIPRRFDPTR
                     AFATPRPIKSLIGPAPRTIADDLWAKLLWAGLNLASSDLPLHGSAASDSNLTENRRCT
                     VGYYPLEMLQALSIVWLFAGLRSDEIIRLRVGCVRKGPPVEEVAAQCWMLDVPVHKTG
                     TAFTKPIDTIVGEAVSAWERARPVQPLALDIKTGEQVEFLFSYRARGIPHHYLNRHLI
                     PLLCRKAGIPSADARGRISSHRARSTIASQLFNAREPMTLFELQAWLGHRSPVSTQHY
                     VAFTPTKLARAYSEAQYFQRNLRMMEVLIDRQAIDSRNPDEPWRYYDLGHGLCSYEFF
                     DQCPHRMACARCDFYVPKESTRAELITSKTGMIRMLQEIPLTEEERNAIEGDQKAVDR
                     LLRRLESMPAPDGSLRRKR"
     gene            complement(9662..10723)
                     /locus_tag="VHV99_14400"
     CDS             complement(9662..10723)
                     /locus_tag="VHV99_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX3636647.1"
                     /translation="MGASNAFVEPVWERFPLIASNCYARRWIQSCTSLGLARNTVAAY
                     MYAMEGFLGFAQDHSIDLRCTSQEAIARYVGDLRTQPSRSGASVTRIDSGGFLSNATL
                     QQRLTAVRLFFEFLVDEGLVRNNPVSRGRHTLSSRFGARGDRSLVQKFHKLPWIPTEA
                     QWMSLLAVVRDEPQRNRCMLAFAYDAALRREELCELRSSDLDPAHRLLRIRAETTKGR
                     RERVVPYSDTSGQLLRDYLLHRRYVASDRQALFLSESPRNRGAPITLWTWSKVVRSIA
                     LRANVPSFSTHTLRHLCLTDLARSGWQLHEIARFAGHQSLETTRQYIHLSGQDLAERL
                     AKTMRQIHSWRVDALAGAD"
     gene            complement(10967..11188)
                     /locus_tag="VHV99_14405"
     CDS             complement(10967..11188)
                     /locus_tag="VHV99_14405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636648.1"
                     /translation="MTTVWVGDVDHLRPGCFSFYCQNASTFASLHGTRARAAHAGRRG
                     AFYGDGPQRLARRASGQAKEKMYPILKKK"
     gene            complement(11207..11578)
                     /locus_tag="VHV99_14410"
     CDS             complement(11207..11578)
                     /locus_tag="VHV99_14410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636649.1"
                     /translation="MPIPSKLQPWFEARQRFRLSHAYIKMARELGLNPRKFGSLTNGQ
                     QEPWKRPLSEFIAHCYHKRFGRTTPEHVQSLEDVVKRAEGRRSERRERKTNRAVPPTG
                     PQRSDDGSSEKLPELCARGGE"
BASE COUNT         2248 a         3460 c         3607 g         2337 t
ORIGIN      
        1 ctgatggcgg tcgatctgcg cggccgcttc gaggaaccgt atccgttctg gaatgcttca
       61 gtcggagcgc cggctgcgcg tctgcgcgac gaccttgagt tgttgccgca gttagccgag
      121 aacgggctgt gtgatgcagc gaaagacatc agcatggcgg gtacgctggg cacggctttg
      181 atgttgctcg aatgttcgca ggtcggcgct cgcatcgatc tcgatgcgat accgaagccg
      241 gctgacgtgc cgctcgcgcg ttgggtcacc gcgtttccga gtttcggcta cctgctctcg
      301 gtgcgtgaat cggacgtcga acgcgtgcag gcgcgatttg aggcgcgctc gctggcctgt
      361 gcggagattg ggacggtaga cgcaacgcgc caggcggtcg tccatcgcgg tgacgatgtg
      421 gcagtgttgt gggacttcag cggcgagtcg tttatcgtcc cggccgatca ggtgccgcga
      481 tgagcatgga cgcattgcgg attgcgctgc tgacgcactc ggtcaatccg cgcggcggcg
      541 ttgtgcatac gctcgagctg gcgagcgcac tcagtgcgca aggacatgac gtcacggtgt
      601 tcgcaccggc tacgaatggc gagacgctat ttcgcacgcc gccatgccgc gtggtgtacg
      661 cgccggtcac ggactcgcag gcaggcaccg ttgcgctggt cgatgcgcgc atccgcgcat
      721 tgaagaggag tgtgcgggcg cacggcccca cggccttcga cgtcttgcac gccgaggaca
      781 gcatcagcgg caatgcactc gccgaactca aggaggaagg tgtgatacgc ggtttcgtgc
      841 gcaccgtgca ccacctcgat cacttcgacg atccgcgtct tgccgcatgg caagcacgcg
      901 cgtaccgcga cgccgaccag gttttctgtg tgagcgacac gtggacacgc gagatgtcag
      961 gtcgttacgg aatcgctgcg gtcacggtgt ctaacggcgt cgacacagca cgttacaccg
     1021 cacaggcgct acccgcagac gaggcgctca ggcaacggct aggactcgga gcggggccgc
     1081 ttatcctcgc ggtgggtggc gtcgaagcgc gcaagaacac gctcgcgctg ctggatggat
     1141 ttgccttgat gcgccgcgcc ttgccgctcg cccagcttgt catcgcgggc ggtgccagtc
     1201 ttctcgacca cgacgggtac acgcagcgtt ttatccagtg cgcggcgcag cacggtttgt
     1261 caatcggaac gcacgaaagc attgtggtca cagggccgct cgaagatgcc gcgatgccgg
     1321 ctttattccg gagcgcggat gtgctggcga tggtgtcggt gcgcgagggc ttcggcctgg
     1381 tcgtcctcga agcgctcgcg tgcggcacgc cggtggtggc gtcggcgatc gcaccgttca
     1441 ccgaatacct cgatgacgcc acctgtttct gggccacccc caccgacgca gactcgattg
     1501 ccgcagcgct gctgcgcgtg attcgcggcg atagcgcaat cgattttgct catgctgtgc
     1561 cagcgctgct tgcccgcttt agctgggacg ctagcgcccg ccgtcatgtc gacgcctatc
     1621 gcgcgtggct ggcacaccag ccgcttgcca ttcactaagc catcagtaaa agaggatttc
     1681 tatgcctgtc atgcacttcc ggattcaatg gcccgacggg gacgaagcaa attgttattc
     1741 gccgtcgcag gtggtcagcg agttttttac gccgggccat gcttatccgc tcgacgattt
     1801 tgtcagacgc tcgcgcgagg cgctcaacat tgcctcggag cgcgtgcggc agaagtacgg
     1861 ctttgcctgt tcggcggcga tggatcagct cgcgcagatc gaacagcagg cagaccgctt
     1921 tcgcaatcga cccgacgcga ccgtcaaggt catcgaactg ctctaaaccc gggatgaacc
     1981 gttgaacctg gtgcgccggg cggcaacgcc gcgacggtga gccgattttt tacttgcaag
     2041 gacgcatcat gtcgaacaca gcgcactcct taagcgccgg gacgcgcgag aacaggcact
     2101 acagcgtgct cgtcgtcggt ggcgggcagg ccggtctgtc gatcagctac tatctgaaac
     2161 gggccggcgt cgaccacctc gtcgtcgaga agaacaccgt cacgcatacc tggcgcaacc
     2221 agcgctggga cgcgttctgc ctcgtcacgc cgaattggca atgcgcgttg ccgggatatc
     2281 cgtacccggg cgacgatccg catggcttta tgaacaagga tgaaatcatc gcgtatctcg
     2341 atggcttcat cgcgatggtc gatgcgccgg taatcgagca cacccaggtc aaacgcgtga
     2401 agcggctgga cgacggcacc tttgcggtag cgacgagcca gggcgatttc accgccgatc
     2461 aggtcgtagt agcctcgggc ggttatcaca cgccgatcgt gccgcgcatg gctgaacgcc
     2521 tgcccgcgca gatcgtgcag ttgcagtcgt cggaatatcg caacccgcag gcgctgcccg
     2581 agggcgcggt gatggtggtg ggatcggggc agtccggcgc gcagattgcc gaggacctgc
     2641 atctggccgg ccgcaaggtg gtgctcgccg tgggcgaggc gccacgctgt gcgcgctttt
     2701 accgcgggcg cgatgtggtg gactggctgg ccgacatgaa gtactacgac atgccggtgg
     2761 aaatgcatcc cttgcgtgaa ggggtgcgtg acaacaccaa tcactatgtg accggccgcg
     2821 acggcggacg cgacatcgat ctgcgcaagt tcgcggccga aggcatggag ttgtacggac
     2881 gactcgacga cctgcagaat ggacacttgc agttcgcgcc taacctcacg gttaatctcg
     2941 acgatgcgga cgatacgtat aaccggatca atgcgagtat cgatgcattc atcgaaaaga
     3001 acggcatcga tgcaccgccc ggcggtgtct atgagccttt atggcagcca cgtcacgagc
     3061 gtacgacgct cgaactcgca ggcagcggca ttacatcgat tatctggtgc atcggcttta
     3121 ccccggactt cagctggatc gacgccccgg tgttcaacgg ccgcggttat ccggcgcaca
     3181 cgcgtggcat cacgccggtt gaggggctct acttcatcgg cttgccgtgg cttcatacgt
     3241 ggggctccgg gcgtttctcg gggatcgcgc gcgatgccgc ctacgtggtg caggcaattc
     3301 tcgcgagcgt ccgcgagcga acgtcctctt gcgacactgt agcggcctga agcgcatgca
     3361 gcaaacgaca cagagctatc gtgccgggat gccacaactg gcctatacgg ggctgtccga
     3421 aaactggctg atcaaggaat gcgggcatcg ccactgggag gcgctggccg gagatgctgg
     3481 ccggcgtgca ccggatttcg tcgatgacga tggaagcaaa tcgtacgcgg ccttcaccgc
     3541 aatcagattg acgggggcaa cgctccacgc aatcggagag aacgacgatt tcgaactcgg
     3601 tactgggctt tgccgaaccg gtccggctcg ccactatagc cagcaccgtc tgctttcggg
     3661 caatggcgag tgcgccatcg tgtcgatgtc gtcgacgttc gtgcggcggc acgaggtgcg
     3721 caacaaccgt tcagtgacgc gcgcgacgtt cgctgcaatg ggaggcccga caccgactcc
     3781 gccggcggat gcagtggaca tggcgcggcg tggcaagcag tttcgcgccg ggcagtggga
     3841 cgagcggttg cccttcacaa gcgccgatcg cgtcgggaac gactggatcg aatttctgcc
     3901 atgtccgaat aacgatttca acggtgcgga ttttctctac tttgcaagct ttcagtcatt
     3961 tgttgaccgt gccgaatggc agcgccaccg gttccacgag ccaccggtta tcgcaggccg
     4021 cgagttgttc ttctacggca acattgatgt tggtgacacc cttggactgc gcgtggtcgc
     4081 ggagcataag gatgaacgcg gcctcgtcca ttggtgcgag cttcgtcgcg gcagcgatca
     4141 gcagaagatt gccgacgtag taacaagcaa gcattggagg cagcgatgaa ccgcgagggc
     4201 gatctgcgcg gcgaccgggg cttcgccgat gaaagtcgcg acgcggtacg gcacccgccc
     4261 aatgcggtac cgcttaaagc gctatacacg gagagcgatc ttgaaggggt gtcgcatctc
     4321 ggcagtcggc ccggtgaggc gcccttcgtg cgagggcccc atgcgtcgat gtatacgcaa
     4381 aaaccgtgga cgatccgtca atacgcaggc tatgcggacg cggccgattc gaatcttgca
     4441 ttccggacgg cgctagccga aggggcacag ggtttatcgg ttgccttcga tctgccgacg
     4501 catcggggct acgattcgga cgatcctgcc gccgtggccg atgtcggcat ggctggagtc
     4561 gcaatcgaca gcgtcgagga catgaagcgc ctgtttcggc agattccgct cgatcgggtg
     4621 tcggtctcga tgacaatgaa cggcgccgtg ctacccgtac tcgctgcatt catcgtcgct
     4681 gcgcaagaaa gtggtgtgca tctggaggcc cttacaggta cgatccagaa tgacatcctg
     4741 aaggaataca tggtgcgcaa tgcctggata ttcgcgccgg agccgtcctt acggatcgtc
     4801 gcggacgtcg tcgagtatct ggccgaacgc gcgccgcgtt tcaatgcaat ttccatttca
     4861 ggttaccact ttcaggaagc cggcgcagac gcggtactcg aactcgcgct gacgctgtcc
     4921 aatgcgcgca cctacgtcga tgccctggtg aagcggggca tgaatgtcga gcgcatcggc
     4981 gaacggctga gtttcttctt tggcgttggc accgaattct ttgtggaagt cgccaaactc
     5041 cgcgccgctc gggtcgtctg ggccgggata atgagcgaac tgggtgcacg tggggctaga
     5101 gcgcaatctt tgcgcatgca ttgccagacg tcgggatggt cgctcactgc gcagcatcca
     5161 cagaacaaca tcgtccggac gacgatcgag gcgatggccg ccgtgttcgg cggtacccag
     5221 tcgctgcata ccaatgcgta cgatgaggcg cttgcactcc ccagcgcgga tgcgtcaagg
     5281 ctcgcgcgtg acacgcagtt gatcatgcag catgagatgg gcctgtgcga tgttatcgac
     5341 ccgtgggcgg gctcgtacat gatggagtcg attaccgacg atattgcccg gcgcgtgcgt
     5401 gatctgatga cgcaaatcgc tacgcaggga ggcgtcgtcg aagcgatccg ctcgggttgg
     5461 gtacagcagc gcattcacga agccgcaaca cgcacgcagg cgcggatcga tgcagggcag
     5521 cgtgtcgtcg tgggtgtgaa ccggtttcga tcaccggatg cggatactcc gtctaactgt
     5581 ctggagatcg acggtcggcg ggttcgtgcc aggcaagttc ggcagctggc agaactgaag
     5641 gcacagcgcg acgatgtcct ggtttcgcac gcgctggcgg cgctcgctga cggtgcgcgt
     5701 gacggcaccg gcaacctgct ggccctcacc atcgagtgtg tccgcgcgcg cgcgacaatc
     5761 ggcgaatgca cgcgcgcgct ggaggccgtc tggccacgct acagcatgcc cagtcgtttt
     5821 agcagcggag tctacggcga agcgcgggtc atcgatgggg catggcgtga cgcatgcgat
     5881 cgcgtcagtg ctcttgccgc gcgcctcgga cgcctgccca gggtgctcat cgcaaaggtg
     5941 gggcaggacg gccacgatcg gggtgcaaag accgtcgcgg cggcgttggc cgatgccgga
     6001 ttctacgtgt cgctcggcgc gctgttccgc tcgcccggag aggtggtcga cgacgcgatc
     6061 gcagcgaagg ccgatgtcgt cggcatttcg tccctatcgg gtgcgcatct tgaccttgtt
     6121 ggcgaggtcg tgcgcacgct gcgggtccgt cgaccgaggg cagcggtggt agttggtggc
     6181 atcattccgg acgagcacgc acgtttgctc aaacgcaatg gcatttgtgc agtgctcgga
     6241 ccgggggcag cgatggagtc gattgtggcg aactttgtcg aagccgtcga ggtggcatcg
     6301 gcatcggccg ttgcgaggtg atatccgaga tacggccgaa tggcgtctcg cgacgcaagc
     6361 gcactatgaa gtcaccttga ttggaggttg gctgggagcg ggaatcgtgt ctgtctgaat
     6421 ttttaatgga caaatgtacg cgatatatga gaccggggtg atgcaggagg ttgtgcaacc
     6481 gccgcgacgt cggcggcatt gcaaggagtt caagacaaca caggtgatcc gcgcggcgat
     6541 gcagccgaac gtgtcgatcg cggcggtagc gcctgccgtc tgaacgccaa catgctgcga
     6601 agctgggtgg ccgcccagga agaactgaat gcgatcgtca gcgcgcgtat ggccagtcat
     6661 tcgtgcagct tccggttgcc tggacggtcg cgtctgctgg aacgcatgcc ggctgcgccc
     6721 gaggcccggt agatggtcat cagctcctta acctggcgcc ggctcggcgg cttgccccgt
     6781 catcagaaca ccatcgacaa gtcaggatcg tcctgagccc gcgaaccccg cgcatggaca
     6841 tggtgatcgt ccaggatgcc gctatgcgag cctctcattg cttggtcagc tcgcatgaga
     6901 cgcgacgttt cagtgttgca actctctcta acggaaaagc tctcctattt gagttgggca
     6961 gttttgcgct gccggcattc acaccgcttg cagcggcacc accaattgcg cggggtcgct
     7021 tactgtgcgc gaatcttgag gcgtcgtggg ccgaatgaga agccgggctt gtttggcgaa
     7081 gctcggtcgc gatgactgtc ttaagccgta agcgggacca tcgcaaatcg ggctcgtgac
     7141 actgcactgg gtcgactccg ggcatcgacg tcacttcaaa gcggtcattg gcagagcata
     7201 gaaagcgatc ctgcgcaaat gaccgcgatg gcagaggacg cgacgcacat catcggtggt
     7261 cgaacggccg ttgacgggag cgaagcagtc gcacgaatgt ccaaacgacg gccgcgacga
     7321 atgactcttc ctggccgact cctgcctgat gccttcaaca tctgtctcac cgcttccttc
     7381 ggagcgagcc atctggtgcc ggcatggatt cgaggcgtcg aagcagacgg tcgacagctt
     7441 tctggtcacc ctcgatggcg ttgcgctctt cttcggtgag cggtatttcc tgcagcatac
     7501 ggatcatgcc ggtctttgat gtgataagtt cagcccgcgt cgattctttc ggaacgtaga
     7561 agtcgcaccg cgcacacgcc atgcgatgcg gacactggtc aaaaaactca taagagcaca
     7621 acccgtggcc cagatcgtag taacgccatg gttcatctgg attccggctg tcgatagcct
     7681 gccgatcaat caatacctcc atcattctca ggttccgttg aaagtactgc gcttcggagt
     7741 aagctcttgc cagcttcgta ggcgtgaacg cgacatagtg ctgcgtgctg actggcgagc
     7801 gatggccaag ccacgcctgc agttcgaaca gcgtcatcgg ttccctggca ttgaagagtt
     7861 ggctcgcgat agtcgaccgc gcccggtggc tgctgatccg gccgcgtgca tctgcagatg
     7921 gaatgccagc cttacgacaa agcagcggaa tcagatgcct gttcaggtag tgatgcggaa
     7981 tccccctcgc acgatacgag aacaggaact caacctgctc gcccgtcttg atgtcgagcg
     8041 caagcggttg tacgggacgc gcgcgctccc atgctgatac ggcttcgcca acaatggtgt
     8101 cgatcggctt cgtaaatgcc gttcctgtct tgtgaactgg aacatcgagc atccagcatt
     8161 gcgccgccac ttcttctact ggcggtccct tccgcacgca gccaactcgt agtcgaatga
     8221 tctcgtcgct acgtagtccg gcaaagagcc atacgatgct caacgcctgc aacatctcaa
     8281 gtggatagta gccaacagtg cagcgacgat tctccgtcag gttgctgtcc gatgctgcgc
     8341 tgccatgcag gggaagatca cttgacgcca gattcagacc ggcccagagg agtttcgccc
     8401 aaaggtcgtc cgcaatggtg cgcggcgccg ggcctatcag tgacttgatc gggcgaggcg
     8461 tcgcaaacgc tcgtgtcgga tcaaatcggc gtgggatcca ttcccattcc tgtgcatcag
     8521 tgaaaaaaca ccgcatagaa cccagcaagg cgttcttcga gcgcgctgtt atcggtttac
     8581 cgagtccatt gcatatacct gcggtgtgcc agatatagtc cccaacacgc atccggtcca
     8641 catcgctaac gtactctgcc gccagttcgc gggtccactg atcggcaccg gtaacctcgg
     8701 gatgccggtc tttcaaccag cgaccggcct tcagaatcat gtttcgaaat tggcggcggc
     8761 taactgccgt cagtgtgctg gtcgcttccc atcgctccac catcgcgacc cagtcggcag
     8821 cgatgcctgc gattctcccc gcagctatga attccctgtc cacggtatcg cgcactttgg
     8881 gttcatctga aactatcccc aatgtcgcaa ggacgcgcgc caactggaag tacagggagc
     8941 tgcgatggtt ggagtcgcgc catcgggcac gaaccgctcc gatgaagtcg gctgtcagcg
     9001 attcaagctc cggccgctga tttataagca ggagctcagc gaccatgctg atcagcggtt
     9061 tgttcgggct gcgataaccc cacgccgtca caacgtccgt tacgcgcgtc aaagttgtag
     9121 agacctgatc cttgccgaag atcttctctg ccagagcaac ccgctttacc tctcccagac
     9181 tggaaatgtc gcgaaaacag cgaagcaaat aggcaacggc gattaatgcc aggcgctcac
     9241 cagcggcgcg cggcttcaca tcgtgcgccg aaacgaagac ctgttggttg gtgcctagaa
     9301 ctttgcgcca aatggtctcg tcccatgccc agaaagttgt ccttcgctct gcacagcctt
     9361 gaagaagtat cagcctgacg cggtcgccac agtcgcgtct gccatcgatc acggcaagcg
     9421 catcattgat cggcgtcagc agccgttcca gaccagccaa ctggccgagc atagtcctca
     9481 ctccgcgctc ggtccacagg tccccggaga gcttgcatgt aagtagctcg tgttctgcag
     9541 ctgtcaggct gctgctgcga tcatagtccg ctattcgtat tggccagagc caggtacggg
     9601 cgccggaggc tgaggtcaga gtgcgcgtgg tatgagatgc cttcatcatc gtctccactc
     9661 gtcagtcagc accagccagg gcgtcaacgc gccacgaatg gatttgacgc attgtctttg
     9721 ccaaccgctc cgccagatcc tgtccggaca gatgtatgta ttgccgcgtc gtctccagac
     9781 tctgatggcc agcaaagcgg gcgatctcgt ggagttgcca gcctgaccgt gcgagatctg
     9841 tcaggcaaag atgacgcagc gtatgggtgg agaacgacgg aacgttggca cggagcgcga
     9901 tggagcggac aaccttggac cacgtccata acgtgatcgg cgctccccgg ttgcggggtg
     9961 actcagacag aaataaggct tgccgatcgc tggcaacata tcgccgatgc agaaggtagt
    10021 cacgtagcag ttgccccgac gtgtcggaat acggcacgac acgctcccgt cggcccttgg
    10081 ttgtctctgc gcggatacgc agcaaccggt gcgctggatc gagatcactg cttcgaagct
    10141 cgcacagttc ctctcggcga agcgctgcat cataggcaaa tgccagcatg cacctgtttc
    10201 gctgcggttc gtcgcggacc acagcaagca gtgacatcca ctgcgcctcc gtaggaatcc
    10261 agggtagctt atgaaatttc tggacgaggc ttcggtcacc gcgagcgccg aaacggctgg
    10321 ataacgtatg tcggccgcgg ctcacagggt tgtttctgac aagcccctca tcgacaagaa
    10381 actcaaagaa caaccggact gcagtgagac gctgctgcag tgttgcgttc gataagaagc
    10441 cgcccgagtc gatgcgagtc acactggcac ccgatcgact cggctgggtg cgcagatcac
    10501 cgacataccg tgctatagct tcttgcgatg tgcagcgcag atcaatggaa tgatcctgcg
    10561 caaagccaag gaagccttcc atcgcgtaca tgtacgccgc gactgtgttt ctcgccagac
    10621 cgagcgatgt acaactttgt atccagcggc gagcgtaaca gttcgaagcg attagcggga
    10681 atcgttccca gaccggttcg acgaaagcgt tgcttgcacc cataagttgc tccctgcgag
    10741 caatgctgcc tcgtcaactg gtaacgaacc aacaggcaaa acgacatcgc gttcgacacg
    10801 ggttatccac cgggccgctc gcctctcgct attgaagaag cgggcggcgg ccccgtggat
    10861 aaccctgccc tttcttgttt caagaggcac ggaatgcgac gccgatggaa aagccgctcc
    10921 accgtagcac ggaatcaatt ccaattaact taaactatct accccttcat tttttcttca
    10981 gaatagggta cattttttcc ttcgcctggc cggaggcccg ccgcgcaagg cgttgcggtc
    11041 cgtcgccata aaaagcgccg cgccgtcccg catgcgcggc tcgcgcacgc gtgccgtgca
    11101 atgaagcgaa agtagaagcg ttttggcagt aaaaggagaa gcaccctggg cgcaagtggt
    11161 ccacgtcgcc cacccagacg gtcgtcatgt ccgttccgtt cgaaccctat tcaccgccgc
    11221 gtgcgcagag ctccggcaac ttttcgctcg atccgtcatc gctacgttgc ggccctgtcg
    11281 gtggaaccgc tctgttcgtt tttctctccc gccgctcgct gcgccgccct tccgcacgct
    11341 tcacgacgtc ctccagagat tgcacgtgtt ctggcgtagt acggccaaag cgcttgtgat
    11401 aacaatgcgc gataaattcc gagagggggc gcttccacgg ttcctgctgc ccgttggtca
    11461 gtgacccgaa ttttcgggga ttcaatccca gttcgcgcgc catcttgata taagcatggc
    11521 tcaatctgaa acgctgcctc gcttcaaacc atggttgaag tttcgatggt atcggcatca
    11581 gtgttcacct ttgcgccagc ttgcggcggt caaccgctcc accgccgcgt aataacgcgc
    11641 ctgagaagtc gc
//
LOCUS       DATASH010000522         6800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6214856,
            whole genome shotgun sequence.
ACCESSION   DATASH010000522 DATASH010000000
VERSION     DATASH010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6800
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6214856"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VHV99_14415"
     CDS             complement(<1..642)
                     /locus_tag="VHV99_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016344472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-alanine antiporter"
                     /protein_id="HEX3636650.1"
                     /translation="MDWLHHILQKSPEIALFLSLAAGYFIGQINFGKFQLGGVGGSLL
                     AAVVISQAGVTIDNGVKSVMFAVFIYAVGYDSGPGFFNSLNRKTLREIAMAIFLAVTA
                     LITVVVCAKLFHLNKGLAAGLAGGALTQSAIIGTAGDAIARLGLPADQVKSLQSDVAI
                     AYAVTYVFGSLGAIIVCVNILPKFMGQGLREASIEAERELSSGTPARAPGQLSA"
     gene            complement(773..2455)
                     /gene="aspT"
                     /locus_tag="VHV99_14420"
     CDS             complement(773..2455)
                     /gene="aspT"
                     /locus_tag="VHV99_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016344471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0070906 - aspartate:alanine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-alanine antiporter"
                     /protein_id="HEX3636651.1"
                     /translation="MIGELLRSQPEIALFASLAIGYFIGSFRVGPIQLGGVCGTLIVA
                     LLLGQTGARLAPDLKNIAFALFIFALGFTGGPQFFANIGRGWRYGLLSVVEIVSVLVL
                     IMIAVFAMRLDAGTAAGLLAGAATESAVIGTASEAIAKLGFSDAQTVRLQANIVTAYS
                     VSYLFGLITIVLFTSQFAPLLLRINLREEAERVWHKLGGDSAFGEGQRAAAPALVGRA
                     FRVGMAAGSTIFTLEQQHGFNLTIERLRRNGADVPVEPHLTLSAGDLILVAGRREAIV
                     AAAASLGEEVADAGFSQFMMEKLDVVLTRGEAHGLTVAQVRERAKPEDGRGVYIAAVT
                     RLETTVPALPGTELNRGDVLTLVGAKADVTRGARHLGYVLAATQKTDFVYLGLGVLVG
                     MAIGHLGGRIGGVSIALGTGGGCLLSGLLFGWIRSRYPLVGSLPSAAAQILKDFGLAT
                     FIAAVGLSAGPDAIKLVREYGLALPVAGILMVLVPGLLSLWIGRVFLKLEAPMLLGAI
                     AGQQCSTPAISALVGVTGNSTPVIGYTITYALSNILLPLMGPVVVGLAGKFG"
     gene            3007..4005
                     /locus_tag="VHV99_14425"
     CDS             3007..4005
                     /locus_tag="VHV99_14425"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013338139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEX3636652.1"
                     /translation="MRMILFSSRQYDRDTFAEANGNHSFELHCQESHLDGETAILAQG
                     YEVVCPFVNDNVDAAVLQRLHAGGTRMIALRSAGFNHVDLAAAERLGMTVARVPAYSP
                     HAVAEHAVGLILALNRRLPRAAARTREGDFSLHGLLGFDLHGKTAAVVGTGMIGLVFG
                     RIMAGFGMRVLAHDPGTPADDLLALGARYVPLDTLLAESDVVSLHCPLVPGTYHLIDG
                     PALAKMKRGAMLINTGRGGLVESNALIGALKDGQLGHLGLDVYEEEGGLFFEDHSNLP
                     LQDDVLARLLMFPNVIVTAHQAFFTREAMNEIAQTTLDNVAAWQAGTPRNAVPPRG"
     gene            complement(4002..4643)
                     /locus_tag="VHV99_14430"
     CDS             complement(4002..4643)
                     /locus_tag="VHV99_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636653.1"
                     /translation="MHLSQSARSVNALLTCWRFLARVSSLPLPLTHSSPSAAPACAET
                     IWTVPLHEHPWYDQVRLKRVFVTDGTRHQVVLVDVRKLLECADRDNTDYVLKPVAEWH
                     SGKVRGIREFLDPDNSRIPQMPYVTISTRRAPGLLGWLGLEREGVVAFRNGQHRARYL
                     ADAGARWFPVEVHEREAALLREMCGAADDARTAIRPTTADGAADAVAGDSPPG"
     gene            4720..5526
                     /locus_tag="VHV99_14435"
     CDS             4720..5526
                     /locus_tag="VHV99_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018431840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEX3636654.1"
                     /translation="MIVTQDFNNHVRIESNSDIGVATIVLDRPARRNAVDRPTAQALS
                     AAFRRFEAEPAWRAAVLFGAGGTFCAGADLTALADETRRNELHADGSGPGPMGPTRMS
                     FSKPVIAAIAGYAVAGGLELAAMCDLRVVEEDAVLGVFCRRVGIPLIDGGTIRLPRLI
                     GLSRALDLILTGRAVAAQEALAFGLANRVVPRGEARAAAERLAAELAALPQAALLADR
                     HSALENSTLDDLALALQREGAGGYPAVFGEGVAGAARFAEGAGRHGDTLK"
     gene            5769..>6800
                     /locus_tag="VHV99_14440"
     CDS             5769..>6800
                     /locus_tag="VHV99_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3636655.1"
                     /translation="MPLPLLALAVAAFGIGTTEFVIMGLLPDVARDLSVSIPAAGMLV
                     SAYALGVTIGAPIVAIAVANMPRKKALMSLIGVFIVGNLLCAIAPSYAVLMAARIVTA
                     FCHGAFFGIGSVVAAGLVAPNRRAQAIALMFTGLTLANVLGVPLGTALGQAVGWRATF
                     WAVTGIGILAAGALAVCLPAKIEMQKASLVREFSVLKNPQVLMVLGISVLASASLFST
                     FTYITPILEDVSGFTPHAVTFVLLLFGLGLTVGSTLGGKLADWRLMPSLVAFLLAIVV
                     ILTIFAGTMHAEIPAMITIFVWGILAFAIVPPLQMLIVDRASHAPNLASTLNQGVFNL
                     GNATGAWLGG"
BASE COUNT         1267 a         2232 c         2072 g         1229 t
ORIGIN      
        1 cgcggacagt tgccccggcg cgcgggccgg tgtacccgaa ctcagttcac gttccgcttc
       61 gatcgaggcc tctcgcaagc cctgccccat gaacttcggc aagatgttca cgcagacgat
      121 gatcgcgccg agcgagccga acacgtaggt caccgcgtag gcaatcgcca cgtcggattg
      181 cagcgacttc acctgatcgg cgggcaaacc gaggcgcgcg atagcgtcgc cggccgtgcc
      241 gataatcgcc gactgcgtca gcgcaccgcc cgccagcccc gcagcgaggc ctttgttgag
      301 atggaacagc ttcgcgcaga ccaccacggt gataagcgcg gtcaccgcga ggaagatcgc
      361 catggcgatc tcgcgcagcg tcttgcggtt caacgagttg aagaagcccg gcccggagtc
      421 gtacccgacc gcgtagatga aaaccgcaaa catcaccgat ttcacgccgt tgtcgatcgt
      481 gacgccagcc tgactgatca cgaccgccgc cagcaacgac ccgcccacac cgcccaactg
      541 gaatttaccg aagttgatct gcccgatgaa gtaacccgcc gccaacgata gaaacagcgc
      601 gatctcaggt gatttctgca aaatgtgatg tagccagtcc atcgtgccct cgctggatag
      661 tttgagatac tgagtactgg atgctgcatg tccacgcgga tttcgtgtcg atgtccgcgt
      721 gccgtgtcgg ctgcgcgcgg cgtggtgcac cgcgcgcagc ccatctggcc gcctagccga
      781 atttcccggc gagaccgacg acaaccggcc ccatcaacgg caacagaatg ttcgagagtg
      841 cataggtgat cgtgtagccg atcacgggcg tcgaattgcc ggtcacgccg accagcgcgc
      901 tgatcgccgg cgtactgcat tgctggccgg caatggcgcc gagcagcatg ggcgcctcga
      961 gcttgagaaa cacccggcca atccagagcg acagcaagcc gggcacgagc accatcagaa
     1021 tgccggcgac gggcagcgcc aaaccgtatt cgcgcaccag cttgatggca tcgggtcccg
     1081 ccgacaaccc gacggcggca atgaaggtgg ccaggccgaa gtccttgaga atttgcgcgg
     1141 cggccgaagg cagcgaaccg actaatggat agcgcgaacg gatccagccg aacagcagcc
     1201 ccgacagcag acatccgccg cccgtgccga gcgcaatcga cacaccaccg atgcgtccgc
     1261 cgagatggcc gatcgccatg ccgaccagca cgcccaggcc gagataaacg aagtcggttt
     1321 tctgcgtcgc ggccagtacg tagcccagat gccgcgcgcc gcgcgtgacg tcagccttcg
     1381 cgcccaccag cgtcagcacg tcgccgcgat tgagttcggt gccgggcagc gcgggcaccg
     1441 tggtttcgag ccgcgtgacg gcggcgatgt aaacgccgcg cccgtcttcc ggctttgccc
     1501 gttcgcgtac ctgcgcgacc gtcagaccgt gcgcctcgcc gcgcgtcagc acgacgtcga
     1561 gcttttccat catgaactgg ctgaaaccgg cgtccgccac ttcctcgcca aggctggcag
     1621 ccgcggctac gatcgcttcg cgccgcccgg ccacgagaat caggtcgccg gcgctcaacg
     1681 tcaggtgcgg ctcgaccggc acgtcggctc cgttgcgcct aagccgctca atcgtcagat
     1741 taaagccgtg ctgctgttcg agcgtaaaga tcgtcgagcc ggccgccatg ccgacccgga
     1801 atgcgcggcc cacgagcgcc ggtgccgcag cgcgctgacc ttcgccgaat gcactgtcac
     1861 caccgagctt gtgccacacg cgctcagctt cttcgcgcaa attgatgcgc aacagcagcg
     1921 gcgcgaactg gctcgtgaac aggacgatgg tgatcaggcc gaacaggtag ctcacgctgt
     1981 acgcggtcac gatgttggcc tgcagacgca ctgtctgcgc gtcgctgaaa ccgagcttgg
     2041 cgatggcctc cgaagccgtg ccgatcaccg ccgactccgt cgcggcgccg gccagcaagc
     2101 cggccgccgt gccggcgtcg agccgcatcg cgaagaccgc gatcatgatc agcaccagca
     2161 ccgacacgat ctcgaccacc gacaacaagc cataacgcca gccccggccg atgttggcaa
     2221 agaactgcgg tccgccggtg aagcccagcg cgaaaatgaa cagcgcaaag gcgatgttct
     2281 tcagatcggg ggcgagccgc gcgcccgtct gacccaatag caaggccacg atcaaggtcc
     2341 cgcagacgcc gccgagttga attggcccca cgcgaaacga accaatgaaa tatccgattg
     2401 caaggcttgc gaacagcgcg atctccggtt gagatctcag caattcaccg atcatgttgg
     2461 ctcgccgatt tacggtttat tagcagaaag ctgaacaaat ctctctggaa tttgaaaatt
     2521 aattcaatcc gggtcgcgac gactcagata ttactcatcc ggcataagca gattccgttg
     2581 aatggaaact ttatggcatt ggcatgacgc ttccggcgtc aaataagata tgccgcactg
     2641 cagcgacagc agggaaacgc ttttcaataa gctcaacagt cacgctgttc ctcgcgagat
     2701 ttgcccgacg ttttgaaaac cacatacttt gcttatggcc cattcttcta ccgaacgaag
     2761 gtgctgtttc gagacgtctt ttttaataat tattgtagtt cgccatgcct gtcaatatta
     2821 ttttcaattg cgaccctttt tgcttcgcaa aacatgatct ttagtaaagc ccggaatcgt
     2881 tttcgaactg cgcatcgcaa gcggcggtca ttcgcataaa agggccgata tactgacctg
     2941 cctccatgcg gcgccgcgaa tgaatcgaaa ttgccgcgcc ggcatgcttt ccttccggag
     3001 ccactcatgc gcatgatcct gttcagcagc cgtcagtacg atcgcgacac gttcgccgag
     3061 gccaacggca accattcctt tgaactgcac tgccaggaat cgcatctcga tggcgaaacc
     3121 gcgatcctcg cgcaaggcta tgaagtggtc tgcccattcg tcaacgacaa cgtcgatgcc
     3181 gccgttctgc aacgtctcca cgcaggcggc acgcgcatga tcgcgctgcg ttcggcgggc
     3241 ttcaatcacg tcgacctcgc cgcagccgaa cgtctcggca tgaccgttgc acgggtgccg
     3301 gcctattcgc cgcacgcggt cgccgaacat gcggtcggtc tgattctggc gctgaaccgg
     3361 cgcttgccgc gcgcggccgc gcgcacacgt gaaggcgatt tttcgctgca tgggctgctc
     3421 ggcttcgacc tgcacggcaa gacggcggcc gtggtcggca ccggtatgat cggccttgtg
     3481 ttcggccgca tcatggcggg cttcggcatg cgggtgctcg cccatgatcc cggcacgccg
     3541 gccgacgacc tgctcgcgct aggcgcgcgc tatgtgccgc tcgatacgct gctggctgaa
     3601 agcgatgttg tcagcctgca ttgtccgtta gtgcctggca cgtaccatct gatcgacggg
     3661 cccgctctcg cgaagatgaa gcgtggcgca atgctgatca acaccggccg cggcggcctt
     3721 gtcgaaagca atgcgctgat cggggcgttg aaggacggcc agctcggcca tcttgggctc
     3781 gacgtgtatg aggaagaagg cggcctgttt ttcgaggacc actcgaattt gccactgcag
     3841 gacgacgtgc tcgcacgcct gttgatgttc ccgaacgtga tcgtcacggc gcaccaggca
     3901 ttcttcacgc gcgaggcgat gaacgagatc gcgcagacca cgctcgataa cgtcgcggca
     3961 tggcaggccg gcacgccgcg caatgcggtg cccccgcgcg gctagccggg cgggctgtcg
     4021 ccggccaccg catccgccgc gccgtctgcc gtggtcgggc ggatcgccgt gcgggcgtcg
     4081 tccgctgcgc cgcacatctc acgcagcaag gccgcctcgc gttcgtgtac ttccaccgga
     4141 aaccagcgcg cccccgcgtc ggcgagatag cgcgcgcgat gctgcccgtt gcgaaacgcc
     4201 accacgccct cgcgctccag accgagccag cccaacagac ccggcgcgcg acgcgtcgag
     4261 atcgtcacgt acggcatctg cggaatgcgc gaattgtccg gatcgagaaa ttcgcggatg
     4321 ccgcgcacct tgcccgaatg ccattcggcg accggcttca acacataatc ggtattgtcg
     4381 cgatccgcgc attccagcag cttgcgcacg tcgaccagaa cgacctgatg ccgcgtgccg
     4441 tcggtgacga agacgcgctt caggcgcacc tggtcgtacc atggatgttc gtgcagaggc
     4501 acggtccaaa tcgtctcggc acaggccggc gcagctgagg gcgatgaatg ggtcaagggc
     4561 aaaggcaggc tcgatacgcg ggctaaaaaa cgccagcacg ttagaagagc atttacgcta
     4621 cgagccgatt gtgaaagatg catgacaacc aacggtatta gtcgcactgc atgagcaccc
     4681 ggcacgagtc accggacgcg aacctttacg gatacaacga tgatagtcac gcaagatttc
     4741 aataaccatg tgcgtatcga gtccaactcg gacattggcg tcgccaccat cgtgctcgac
     4801 cgtcccgcgc ggcgcaatgc cgtcgaccgt cctaccgcgc aagcgctgag cgccgccttc
     4861 cgccgcttcg aagcggaacc ggcatggcgc gcggcggtgc tgttcggtgc gggcggcacc
     4921 ttctgcgccg gcgcggatct caccgcgctc gccgacgaga cccgccgcaa tgaactgcac
     4981 gccgacggca gcggccccgg tccaatggga cccacgcgca tgagcttcag caaaccggtg
     5041 atcgccgcga ttgccggcta tgcggtcgcg ggcggcctcg aactggccgc gatgtgcgac
     5101 ctgcgcgttg tcgaagaaga cgccgtgctc ggtgtcttct gccggcgcgt cgggattccg
     5161 ctgatcgatg gcggcacgat ccgcttgccg cgtctgatcg ggctgtcgcg cgcgctcgac
     5221 ctgattttga ccggtcgcgc ggtggcggcg caggaagcgc tcgccttcgg gctcgccaac
     5281 cgtgtcgtgc ccaggggcga agcacgcgcg gcggctgaac gactggccgc cgagttggca
     5341 gccttgccgc aggcggcgct gctggcggac cgccactccg cgttggaaaa cagcacgctc
     5401 gatgatctcg ctctggcgtt gcagcgcgaa ggcgctggcg gctatccggc ggtattcggc
     5461 gagggcgttg caggtgccgc acgtttcgcc gaaggcgccg gccggcacgg cgacaccctc
     5521 aagtgagatg aaagcacgcc ataagacagc gccgtttcgc gagcaaaggg aacggcaagg
     5581 cggcacggca aaaagcgccc tccgtatgtg ctttcgaaaa acaacatccg cctcgacgcc
     5641 cagcaaggct gttttgactt aggtagaatc ccctactgct ttcccctcgc atcgcgcatg
     5701 cgttctcgcc ccgaaaacgc gcgcctgccg cacgacgctt cccgtcgcgc cgatcacgct
     5761 ccgtcaccat gcctttaccc ctgctcgccc ttgccgttgc cgcttttgga atcggtacca
     5821 ctgaattcgt gatcatgggg ttgctgcccg atgtcgcgcg cgatctgtcc gtgtcgattc
     5881 cggcggccgg catgctggtg tcggcttatg cgctcggtgt caccatcggc gcgccgatcg
     5941 tcgcgatcgc cgtggccaac atgccgcgca agaaggcgct gatgagcctg atcggtgtct
     6001 ttatcgtcgg caatctgctg tgcgcgatcg cgccgagcta cgcggtgttg atggccgcgc
     6061 gcatcgtcac ggcgttttgc catggcgcgt tcttcggcat cggttcggta gtcgcggcag
     6121 gactcgtcgc accgaatcgc cgcgcgcaag ccatcgcgtt gatgttcacc ggcttgacgc
     6181 tcgccaatgt gctcggcgtg ccgctcggca ccgcgctcgg ccaggcggtg ggctggcgcg
     6241 ccaccttctg ggcggtcacc ggcatcggca ttctcgcggc gggcgcgctc gcggtatgcc
     6301 tgccggcgaa gatcgagatg cagaaggcga gcctcgtgcg cgaattcagc gtgctgaaaa
     6361 acccgcaagt gctgatggtg ctcggcatca gcgtgctcgc gtcggccagc ctgttttcca
     6421 cctttacgta catcacgccg attctcgaag acgtcagcgg tttcacgccg catgcggtca
     6481 cgttcgtgct gttgctgttc ggcctcggct tgacggtggg cagcacgctc ggcggcaagc
     6541 tcgcggactg gcggctgatg ccctcgctgg ttgcgtttct gctggcaatc gtcgtgatcc
     6601 tgactatttt cgccggcacg atgcacgctg aaatcccggc catgatcacg atcttcgtgt
     6661 ggggcattct ggccttcgcg atcgtgccgc cgctgcaaat gctgatcgtc gaccgcgcca
     6721 gtcatgcgcc gaatctggca tcgaccctga atcaaggtgt gttcaatctc ggcaatgcaa
     6781 cgggcgcgtg gctcggcggc
//
LOCUS       DATASH010000523         8280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_83578,
            whole genome shotgun sequence.
ACCESSION   DATASH010000523 DATASH010000000
VERSION     DATASH010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 8280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8280
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83578"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1437)
                     /locus_tag="VHV99_14445"
     CDS             complement(<1..1437)
                     /locus_tag="VHV99_14445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018423302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX3636656.1"
                     /translation="MSLMERFIKRRIATSFLAIAVLLAGAVAYFLLPVAPLPQVDFPT
                     IQIVAKLPGASAETMATSVATPLERQLSLIAGITQMTSSSSLGTTNIAVQFDLSRDIN
                     GAAQDVQTAINAAGGTLPKNLPNPPTYEKVNPADFTLLSLALTSPSLTLTQLDSYAED
                     YLAQQISQMPGVGLVDFHGQQRPAVRIQLDPDKLTARGLTLEDVRSIVGLQTVNAPKG
                     SLNGPDRSVIFNATDQITTASQYRDLVVAYKNGAPIRVSDLGTVLNAAEDTQQAAWLQ
                     HERAIILDIHKQPGYNVVQTIANIKAKLPALEASLPAAAHLHVVGDRTQTINASVHDV
                     QFTLMLTIALVVMVIFSFLRNVWATIIPSQTIPLSLVATFGVMYLLGYSLDNLSLMGL
                     TIAVGFVVDDAIVVIENVMRHIEEGVPRMKAALLGAMEVRFTIISMTISLIAVFIPIL
                     LMGGIVGRLFREFAVTVSAAILMSALVSL"
     gene            complement(1434..2645)
                     /locus_tag="VHV99_14450"
     CDS             complement(1434..2645)
                     /locus_tag="VHV99_14450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX3636657.1"
                     /translation="MKPADLPNHEAPRARRRAPLVVACVVLVVILVAWLWPRHPAVKP
                     AAAVPVNGGHPVKSDFPIRVDAVGTVLALNTVDVKVRVDGQLQRVSFTEGQDVKAGQL
                     LAQLDQGPLTAQLHQAEAAQRKDQASLDNARLDLARYSKLVGIGAATTQAVDTAKAQV
                     EALAATVAADAALVQSDRLQLSFTTLFAPFTGRVGAREADIGAIVHPADTTGIVTVTQ
                     MEPITVLFSVPQDVLPDLLANQAAAPLAVNVTTRSGSTALADGTLVFIDSTVDPTTGQ
                     IKLKASFTNKDRKLWPGELVNARVLLRTDTQQLAVPSRAVVNTQNGTQLYVINSNGTA
                     QMRPVQTGVTVDGMTEIRSGIAPADLVVFDGQSRLNPGVRVAATTVPTVAQAAANANS
                     AGSQTHQDNPS"
     gene            complement(2642..4123)
                     /locus_tag="VHV99_14455"
     CDS             complement(2642..4123)
                     /locus_tag="VHV99_14455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEX3636658.1"
                     /translation="MSGLSRSAPFSPVVSPRAPHLTLRAAVCGTVCALGVVALAGCSL
                     VPAYQRPSAPLAQHFDGSASAPLADTVPVKSGWWREYHDPALDALIDRSLRNNFTLAS
                     AIASVEQARGNAEKAGAPQYPSLSLGATFDRSHQGRGSTTKTQSLFAEASYEVDFWGF
                     NAANASAADLLARASGFDRDTVALTLTASVADTYFQVQSLRRRLAIARSISDDAAHIL
                     QLLLAQQEAGVATELQVQQQRNALATFQAAVPALQQQLDTSIHALAVLVGTAPEQFTV
                     SDAALADIAIPQPRPDLPASLLETRPDIRSKEAQLQSANESVGAARAAFLPNIVLTAD
                     GGLSSKSLSHFLSSPFASLATSLAAPLFDGGALHGQLHASQAAEAKGVADYRQAVVTA
                     LQDVEDSLSAARQQQLAEAADQTAADAARKAATLAQAQYKLGTVDFLTVLDAQRTLYQ
                     AEDTLVQVRLARLQASVSLFRAFGGGFGVTDSSTASTSPLSRS"
     gene            complement(4283..4960)
                     /locus_tag="VHV99_14460"
     CDS             complement(4283..4960)
                     /locus_tag="VHV99_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018423299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3636659.1"
                     /translation="MRILLVEDDTRASQFLARGLSESGFIVDTVADGATGLAYAREGI
                     YDVIVTDRLLPALDGIALVQQLRAGGDTTPVLMLSAVGGLNERVEGIRAGCDDYLMKP
                     YAFVEVLARIEALARRADRSRMSERLECADLVLDTRARTATRGGRDLRLQHREFLLLE
                     CLARREGQVVTRSMLLEAAWNYDFEPRGNIIDMHVHRLRAKVDRDFPVALIHTVVGAG
                     YVLNPAH"
     gene            5122..5805
                     /locus_tag="VHV99_14465"
     CDS             5122..5805
                     /locus_tag="VHV99_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018423298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3636660.1"
                     /translation="MSNVLVVEDDGQTLAEIVEALTDHGCKVQAANTGRDGLMLAVAN
                     SYDAIVLDRMLPGGLEGLGMLAALRSAGVLTPVLILSALSAVDERVRGLRAGGDDYLT
                     KPFEFIELTARLDALMRRREAAPGVQEYQVGPLRLDPLKREVSCAGRPVPLLPREYRL
                     LEYLMQHEGQVVSRTMLFEAVWDYRLDERTNVIDVHISRLRKKLDPDGTAPMIDTVRG
                     SGYMLHAPD"
     gene            5792..7147
                     /locus_tag="VHV99_14470"
     CDS             5792..7147
                     /locus_tag="VHV99_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018423297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3636661.1"
                     /translation="MRLTELHRTTGFRLATLFLMLFGVIAILLFGYLYREITGFEQER
                     IDDWLIREHAQLQREEPDELVARFEHQNQFDPRRQRPFGLFDATGKHLAGGYAGDQPA
                     IPVFDKPFSMRLVKLPGHPPGRCIAARVPDSRIALQCQNTRELDHFDEELLHALLSTA
                     MITLVIGLVGASLIGISAMRRLDAVTASIEEIVAGNLSRRLPVHSKRDDVDRLVGVVN
                     GMLDEIERLMQEVKGVCDAIAHDLRTPLTRMLGGLERAQRRAKTEAEYRAAIDETIVE
                     TSGLLRTFNALLRISEIESGVRRAGFTTVDLAAVADDVFEFYDPTAEDKQIAFELQRT
                     VSGPLDIQGDPNLLFEALANLVQNAIKFAPHGGHVRITVFRDKTGAGVTVSDDGPGIA
                     AAEQQAVLRRFYRGEASRHTPGSGLGLSLVLAVAGMHGMSICFKDAMPGCEVLFYKPA
                     E"
     gene            7358..7765
                     /locus_tag="VHV99_14475"
     CDS             7358..7765
                     /locus_tag="VHV99_14475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636662.1"
                     /translation="MLSILHGLLDLGWATAGGGSPVTTMTRPGGGQPSLRWRLLLRTQ
                     AFEFARCAITGAAVQAAYVVPALQEGEDAPPGLIMGIEVFAHEQLGFERAEERLAHRI
                     VIGVACGAHRWLHACLAAAASEGNRRMLTPLSK"
     gene            8162..8280
                     /locus_tag="VHV99_14480"
                     /pseudo
     CDS             8162..8280
                     /locus_tag="VHV99_14480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701750.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1534 a         2577 c         2662 g         1507 t
ORIGIN      
        1 cagcgagacc agcgccgaca tcaggatcgc cgcgctcacc gtcaccgcga attcgcggaa
       61 caggcgtccg acaatgccgc ccatcagcag gatcggaatg aacacggcga tcagcgagat
      121 cgtcatcgag ataatagtga aacgcacttc catcgcgccg agcagcgcgg ctttcatgcg
      181 cggcacgcct tcctcgatat gccgcatcac gttttcgatc acgacgatcg catcgtccac
      241 cacgaaaccc accgcgatgg tcagccccat cagcgagaga ttgtcgaggc tatagccgag
      301 cagatacatc acgccgaacg tcgcgaccag cgacaaagga atcgtctggc tcggaatgat
      361 cgtcgcccac acgttgcgca gaaacgagaa gatgaccatc acgacgagcg cgattgtcag
      421 catcagcgtg aattgcacgt catgcaccga cgcattgatg gtctgcgtgc gatcgcccac
      481 cacatgcaga tgcgcggccg cgggcagcga cgcttcgagc gcgggtagct tcgccttgat
      541 gttggcgatc gtctgcacga cgttgtagcc cggctgcttg tgaatatcga ggatgatcgc
      601 gcgctcgtgc tgaagccatg cagcctgctg ggtgtcctca gcggcgttaa gaacggtgcc
      661 gagatccgat acgcgaatcg gtgcgccatt tttgtacgcg accacgagat cgcgatactg
      721 cgaggcggtc gtgatctgat cggtcgcatt gaaaatcacc gagcgatccg ggccgttcag
      781 gctgcccttc ggcgcgttca cggtctgcaa gccgacgatc gagcgcacat cttccagcgt
      841 gaggccgcgt gcggtgagct tgtcgggatc gagctggata cgcacggcag gccgctgctg
      901 gccgtggaaa tcgacgaggc ccacgcccgg catctgcgaa atctgctgcg ccagatagtc
      961 ctcggcgtag ctgtccagtt gcgtgagcgt gagcgacggc gacgtcagcg ccagtgacag
     1021 caaggtgaag tcggccggat tgaccttttc ataggtcggc gggttcggca agttcttcgg
     1081 cagggtaccg ccggccgcgt tgattgctgt ctgcacgtcc tgcgccgcgc cgttgatatc
     1141 gcgtgacaga tcgaactgca cggcgatgtt ggtggttccc agcgagctcg acgacgtcat
     1201 ctgcgtgatg cccgcaatca acgagagttg ccgctcgagt ggcgtcgcca ccgaagtcgc
     1261 catggtctcc gcgctcgcgc ccggcaattt cgccacgatc tggatagtcg gaaagtcgac
     1321 ttgtggcaag ggcgcgaccg gcagcagaaa atacgccacc gcgcccgcca gcagcaccgc
     1381 gatggcaaga aaactggtcg caatgcgccg cttgatgaac cgttccatca ggctcatgac
     1441 ggattgtcct ggtgagtctg actgcccgcc gagtttgcgt tggctgctgc ctgggccacc
     1501 gtcggcaccg tagtcgccgc cacgcgcacg ccagggttca agcggctttg tccatcgaac
     1561 acaacgaggt ccgcgggcgc gatgccgctg cgaatttccg tcataccgtc gaccgtcacg
     1621 ccggtttgca ccggacgcat ctgcgccgtg ccgttcgagt tgatcacgta aagctgcgtg
     1681 ccgttctgcg tgttcaccac cgcgcggctc ggcacggcga gctgctgcgt atcggtgcgc
     1741 aacagcacgc gtgcattcac cagctcgccc ggccacagct tgcggtcctt gttggtgaac
     1801 gaggccttga gtttgatctg accggtggtc ggatcgaccg tgctgtcgat gaacacgagc
     1861 gtgccgtcgg cgagcgctgt gctccccgaa cgcgtggtga cgttcaccgc caaaggcgcg
     1921 gccgcctgat tcgcgagcaa gtcgggcagc acgtcttgcg gcacggagaa cagcacggtg
     1981 atcggctcca tctgcgtaac ggtgacgatg ccggtcgtgt cggcgggatg tacgatcgcg
     2041 ccgatgtcgg cctcacgcgc gcccacgcgg ccggtgaacg gcgcgaagag cgtggtgaag
     2101 ctcagttgca ggcggtcgct ctgcaccagc gctgcgtctg ccgcaacagt ggcggcgagt
     2161 gcttcgactt gcgccttcgc ggtgtccacg gcctgggtgg ttgccgcacc gatgccgacc
     2221 agcttcgagt aacgcgcgag atcgagcctc gcgttatcga gcgatgcctg gtccttgcgt
     2281 tgcgccgctt ccgcttgatg caattgcgcg gtaagcgggc cctgatcaag ttgcgcgagc
     2341 aactgcccgg ccttcacatc ctgcccttcg gtgaaggaca cgcgctgcaa ctggccgtcc
     2401 acacgcactt tgacgtccac ggtattgagc gccaggaccg tacccactgc gtccacgcga
     2461 atcggaaagt cgcttttcac cggatggccg ccgttgaccg gcaccgcagc cgcgggcttc
     2521 acggccggat gcctcggcca tagccacgcg acaagaatca cgacaaggac aacgcacgcc
     2581 accacgagcg gcgcgcgacg gcgcgcgcga ggcgcctcgt ggttcggaag gtctgcaggt
     2641 ttcatgagcg ggaaagcgga gaagtggaag cggtgctgct atcggtcacg ccgaagccgc
     2701 cgccgaacgc gcgaaacagg ctcaccgacg cctgcaggcg cgcgagccgc acctgcacca
     2761 gcgtgtcttc ggcttgatag agcgtgcgtt gggcatccag tacggtcaga aagtcgacgg
     2821 tgccgagctt gtattgggct tgcgcgagcg tcgcggcttt gcgggcggcg tcggcggcgg
     2881 tctggtcggc ggcttccgcg agctgctgct gtcgcgccgc cgacagcgaa tcctcgacgt
     2941 cctgcagcgc agtcacgacg gcctgccggt aatcggcaac gccctttgct tcggcggcct
     3001 ggcttgcgtg caactggcca tgcagtgcgc cgccgtcgaa cagcggcgcg gccagcgagg
     3061 tggcgaggct cgcaaacgga ctcgacagga aatgcgaaag ggacttgctg ctgagtccgc
     3121 cgtcggcggt cagcacgatg ttcggcagga aggccgcgcg cgccgcgccg acgctctcat
     3181 tagccgactg caactgcgct tccttcgagc ggatatcggg acgcgtttcc agcaggctgg
     3241 ccggcagatc cggacgcggt tgcggaatgg cgatgtcggc gagcgccgca tcactcactg
     3301 tgaactgctc gggcgcggtg cccaccagca cggcaagcgc atgaatgctg gtgtccaatt
     3361 gctgttgcaa cgccggcacc gcggcctgga aggtggcgag cgcattgcgt tgctgctgca
     3421 cctgcagctc ggtcgcgacg cctgcctcct gttgcgcgag caacaattgc agaatatgcg
     3481 cggcgtcgtc ggagatggag cgtgcgatcg ccagacggcg gcgcaggctc tgcacctgga
     3541 aatacgtgtc cgccaccgat gcggtcagcg tcaacgccac cgtatcgcga tcgaacccgg
     3601 acgcgcgcgc aagcaggtcc gccgcgctgg cgttggcggc gttgaagccc cagaagtcga
     3661 cctcgtaact ggcttcggca aacaggctct gggtcttggt ggtcgagcca cgcccctggt
     3721 ggctgcggtc gaaggtggcg ccgagcgaca gcgaaggata ctgcggtgcg ccggctttct
     3781 cggcgttgcc gcgcgcctgt tcgacgctcg caatcgccga agccagcgtg aagttgttgc
     3841 gcagactgcg atcgatcagc gcatcgagcg cggggtcgtg atattcgcgc caccagccgc
     3901 ttttgaccgg aacggtatcg gcgagcggcg cactcgccga gccgtcaaag tgctgcgcga
     3961 gcggcgcact gggccgctga taggccggca cgagcgagca cccggccaga gccacgacac
     4021 ccagcgcgca gactgtgccg caaacggcag cgcggagcgt cagatgaggc gcacgaggac
     4081 tgacgacggg cgagaacggc gccgaacggg acagccctga cattggagaa cgctccggca
     4141 agatgggaat gtcgccagat tagtcagcgg gtcctgtcgg cgctttcgcc caatgatgaa
     4201 aaaaatttaa tgcagagcaa catgcggacg gagcggcctg cgcgaggccg tcaacgtcgc
     4261 ccaaaggtgc ccgcacaggt gcctaatgtg ccgggttcag cacataacct gcacccacca
     4321 ccgtatgaat cagcgctaca ggaaaatcgc gatccacctt cgcccgcagt cgatgcacgt
     4381 gcatatcgat gatattgccg cgcggttcga aatcgtagtt ccacgccgcc tcgagcagca
     4441 tgctgcgcgt cacgacctgg ccttcgcggc gcgccaggca ttcgagcaac agaaactcgc
     4501 gatgctgaag gcgcagatcg cggccgccgc gcgtcgcggt tcgtgcccgc gtgtcgagca
     4561 ccagatccgc gcattcaagc cgttcgctca tccggctgcg atccgcgcgc cgcgcgagcg
     4621 cctcgattct cgccagcact tcgacgaagg catacggctt catgaggtaa tcgtcgcaac
     4681 cggcgcggat gccctccacg cgttcgttca gcccgccaac cgcggacagc atcagcaccg
     4741 gcgtggtatc gccaccagcg cgcaattgct gcacgagggc gatgccgtcg agcgccggca
     4801 ggaggcgatc ggtcacgatc acgtcgtaaa tgccctcgcg cgcataggcg agcccggtcg
     4861 caccgtcggc cacggtgtcg acaatgaagc cgctctcgct caagccgcgc gccaggaact
     4921 ggctggcgcg cgtatcgtct tcgaccagca ggattcgcat gcgctaaaca ggttgataat
     4981 ggcttacatt aaaatttttt aatgttggcg cgaaacgcct gtgcagtttc gcgcggcata
     5041 ctttggtgca tcgtaggtac gagcgtagtg tatatgacca gctcgcacag gctcgccgcc
     5101 cgctcacttc tggaaaaacg catgtcgaac gtattggtcg tcgaagacga cgggcaaacc
     5161 ctcgcggaga tcgttgaagc gctcaccgat cacggctgca aggtgcaagc cgcgaacacg
     5221 ggccgcgacg gtttgatgct cgcggtcgcc aacagttacg acgccatcgt gctcgaccgc
     5281 atgttgccag gcggcctcga aggcctcggc atgctggcgg cgttgcgcag cgccggcgtg
     5341 ctcacgccgg tgttgattct gagcgccctg tccgcggtcg acgagcgcgt acgcggtttg
     5401 cgcgccggcg gcgacgatta tctgaccaag ccgttcgaat tcatcgaact gaccgcgcgg
     5461 ctcgacgcgc tgatgcgacg gcgtgaagcc gcgcccggtg tgcaggaata ccaggtcggc
     5521 ccgttgcgtc tcgacccgct caagcgcgaa gtaagctgcg caggccgccc ggtgccgctc
     5581 ttgccgcgcg aataccggct gcttgaatac ctgatgcagc atgagggcca ggtggtgagc
     5641 cgcacgatgc tgttcgaagc ggtatgggat taccggctgg acgagcgaac caatgtgatc
     5701 gacgtgcaca tcagccgcct gagaaagaag ctggatccgg acgggacggc tccgatgatc
     5761 gataccgtgc gcggttcggg gtacatgctc catgcgcctg actgaactgc accgcaccac
     5821 cggttttcgc ctcgccacgc tgttcctgat gctgttcggc gtcatcgcga tacttttatt
     5881 cggctacctg tatcgcgaga tcaccggctt cgaacaggag cgcatcgacg actggctgat
     5941 tcgcgagcac gcacagttgc aacgcgaaga gccggacgaa ctggtcgcgc gattcgaaca
     6001 tcaaaatcag ttcgatccgc gccgtcagcg tccgttcggc ctgttcgacg ctaccggcaa
     6061 acatcttgcc ggcggctacg cgggcgatca accggctatt ccggtcttcg acaaaccgtt
     6121 ttccatgcgg ctcgtgaagc tgcccggtca cccgcctggc cgatgcatcg ccgcgcgcgt
     6181 gccggatagc cgcattgcgt tgcaatgcca gaacacacgc gagctcgacc acttcgacga
     6241 agagctgttg cacgcactgc tttccaccgc catgatcacg ctcgtgatcg gcctggtcgg
     6301 ggcgagcctg atcggcattt ccgcaatgcg gcggctcgac gcggtgaccg cctcgatcga
     6361 ggaaatcgtc gccggcaatc tcagcaggcg tctgccggtg cattcgaagc gcgacgacgt
     6421 cgatcgcctg gtcggcgtcg taaatggcat gctcgacgag atcgagcggc tgatgcagga
     6481 ggtcaagggc gtgtgcgacg cgattgcgca cgatctgcgc acgccgctca cacggatgct
     6541 cggcggactc gaacgtgcgc agcgccgcgc caaaactgaa gcggagtatc gcgcggcaat
     6601 cgatgaaacc atcgtcgaaa ccagtgggtt gctgcgcacc ttcaacgcgc tgctgcgcat
     6661 ttcggaaatc gaaagcggcg tgcgccgcgc gggattcaca acggtcgatc tggctgccgt
     6721 tgccgatgat gtgttcgagt tctacgatcc gactgccgag gacaagcaga tcgctttcga
     6781 actgcagcga acggtatcgg ggccgctcga catccagggt gatccgaacc tgctgttcga
     6841 ggcgctggcg aatctagtgc aaaacgccat taaattcgca ccgcatggcg gccatgtgcg
     6901 gatcaccgtg ttccgcgaca agaccggtgc gggcgtcacc gtttcggacg acgggccggg
     6961 cattgcggcc gccgaacagc aagccgtgtt gcggcgcttt tatcgcggtg aggcgagccg
     7021 tcacacgccc ggcagcgggc tcggcctgag ccttgtgctg gcagtggccg gcatgcacgg
     7081 catgtcgatc tgtttcaagg acgcgatgcc tggatgtgaa gtcctgttct ataaacctgc
     7141 tgaatagcgc aatatgcatg atcgacgcac acggccatgg tcgcctgctc tgacgagcga
     7201 gcgccgtttc gaacggcagc gtttgaaagg taagcgcacg gggaaccctt cttgagttgc
     7261 gttggatgtc ttgggagcat cgtgtccact taacaatgaa gcgaccccgg aatgaggttg
     7321 acccggttgg acagacagat ttgcagttgg gtttaatgtg ttgtcgatcc tgcatggatt
     7381 gttggatctc gggtgggcca cggccggcgg cgggtcgcct gtcacgacga tgacgcggcc
     7441 cggcggtggg caaccctcgc tgcgatggcg tttgctcctg cgcacgcagg ctttcgaatt
     7501 cgcgcggtgc gcgataaccg gcgctgctgt gcaggcggcg tacgttgtac cagccctcca
     7561 ggaaggagaa gatgcgccgc ctggcctgat catgggtatc gaagtgttcg cgcatgagca
     7621 gctcggcttc gagcgtgccg aagaacgact cgcacatcgc attgtcatag gcgtcgcctg
     7681 cggtgcccat cgatggttgc acgcctgcct cgcggcagcg gcgtccgaag gcaatcgacg
     7741 tatgttgaca cccctgtcca aatgatggat cacgcccacg ggcctccgct gcgacaatgc
     7801 catgtcagtg ctctcgacac tcgtaggcat cacccgatgc ggatcatttc gtccggccga
     7861 tgcactgcca tgggaggccg agaaccagcc cggccagctt gttcgtgccc cggatcgtcg
     7921 acgtgccggg tatgcaacag ccaatcggac cagatatcgg attgctgctg ggaggcgcga
     7981 ctattcatgt tgggcgccat tgtcgcttgg ataacgaggg ttgtcgaatg accgaggact
     8041 cgaacatcgt cccggatcag cccacacata gttgggcgtt actctcggcc actaaacgta
     8101 tgggaggtta tcttagtctt actgtgtcat aaacgtaaca atgacgatat tgctgtgttg
     8161 tagtgtcgaa ccgatggccg caagcgtgga tccgcttcac gccagcgccc ggcatcaggc
     8221 tttgcaccac tttgtggcca aagccgactg gtcggacgac gagctacttc gccgggtcag
//
LOCUS       DATASH010000524         3048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4831111,
            whole genome shotgun sequence.
ACCESSION   DATASH010000524 DATASH010000000
VERSION     DATASH010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4831111"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1659
                     /locus_tag="VHV99_14485"
     CDS             <1..1659
                     /locus_tag="VHV99_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008354290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3636663.1"
                     /translation="LRFDVRDTGIGIPRDKQDLIFQAFQQADGTTSRKYGGSGLGLSI
                     SREFSRLLGGSVSVSSEPGKGSVFSVTLPLAVREGTVTAKLDVIGHMSEPETAAANDA
                     PMVAAANESAALVQNHAPAHFAATAPASSTPEPSSWRAPNANFPAPQPIADDRANRKR
                     PDRVILAVEDDLRFAHILHDLAHELEFDFVHATTATQGVELARTLQPNGILLDVGLPD
                     QSGLTVLEWLKHDPLTRHIPIHIVSSTDHADVALHLGAIGYTLKPSARDTLAGAIRKL
                     EARSAQGTRRVLVVEDDPVLRQSIHALLKSATVEIVEAGTIAEALEQMNRVSFDCIVM
                     DLALPDGSGYDLLERVSTSQEHASPPVIVYTGRVLSPDEEQRLRRYSRSIIIKGAKSP
                     ERLVDEVTLFLHSVESALPPEQQRMLRAARQRDDVFEGRTILLAEDDVRNIFALSHVI
                     EPLGAKLEIARNGREALDSLARHPQIDLVLMDVMMPEMDGLTAMTEIRKNPDHTRLPI
                     IALTAKAMASDRQKCLEAGADDYISKPIDVDKLVSLCRVWLRQR"
     gene            1672..2514
                     /locus_tag="VHV99_14490"
     CDS             1672..2514
                     /locus_tag="VHV99_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014251631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEX3636664.1"
                     /translation="MPHRTLKNTEEAVFDIELKLLLEAVYLKYQHDFRHYSVSSLRRR
                     LTQALHDLGLTTLSQLQERILRDATLFSRLFQYLTVQVSEMFRDPPYFRAIREQVVPI
                     LQTYPSIKVWVAGCSSGEELWSLAILFAEENIADRTVLYATDINADALRQAENGIYPL
                     DRLASFSQNYLAAGGKRSLSDYYHAAYGAAKFSQTLKSRVVFADHSLATDSVFLEAHL
                     VSCRNVLIYFDRALQDRALGLFSDSLVRRGFLGLGSKESLRFSKHAGGFADFDARERL
                     YQKV"
     gene            2517..>3048
                     /locus_tag="VHV99_14495"
     CDS             2517..>3048
                     /locus_tag="VHV99_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016354193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEX3636665.1"
                     /translation="MTLSTDSLDAIEAVVIGASAGGVEALNHLLPALPANFRPAVLVV
                     VHVRSDPPSLLPALFAARCKLPVVEPDDKHEIVGGTIYVAPPGYHMLVEPDCTISLSV
                     DPPVRFSRPSVNVLFESAALCFADTLLGIVLSGANDDGARGLVAISRAGGRCWVQDPH
                     TAVANAMPLGAIARGGV"
BASE COUNT          545 a          940 c          968 g          595 t
ORIGIN      
        1 ttgcgtttcg atgtgcgcga taccggcatc ggcattccgc gcgacaagca ggacctgatt
       61 ttccaggcgt ttcagcaagc cgatggcacg accagccgca agtacggcgg cagcggtctg
      121 ggtctgtcga tatcgcggga attctcgcgg ttactgggcg gttcagtgag cgtatcgagt
      181 gagccgggca agggcagcgt gttttcggtc acgctgccgc tggctgttcg tgaaggcaca
      241 gtcaccgcca agcttgatgt gatcgggcat atgagcgaac cggagactgc cgcggcaaac
      301 gatgcgccca tggtcgcggc cgcgaacgaa agcgccgccc tcgtgcagaa ccacgcgccc
      361 gcgcatttcg cggcaacggc tccagcttct tctactcctg agccgtcgtc ctggcgcgcg
      421 ccgaatgcga acttcccggc gccgcaaccc atagccgatg accgcgccaa ccgcaagcgc
      481 ccggaccgcg tgatcctggc ggttgaagac gacttgcgct ttgcgcacat cctgcacgac
      541 ctcgcgcatg aactcgaatt cgacttcgtg cacgcgacca ccgcgacaca aggcgtcgaa
      601 ctggcccgca ctttgcagcc taacggcatc ctgctcgacg tgggcttgcc cgaccagtcg
      661 ggcctgaccg tgctcgaatg gctcaagcat gatccgctca cacggcacat cccgattcac
      721 atcgtttcat cgacggatca tgcagacgtc gcgctgcatc tcggtgcaat tgggtacacg
      781 ctcaaaccca gtgcacgcga cacgctcgcc ggcgcgatcc gcaagcttga agcgcgctcg
      841 gcgcaaggca cgcggcgcgt gctggtggtc gaggacgacc ccgtgctgcg ccagagcatc
      901 cacgcactgc ttaagagcgc gacggtcgag atcgtggaag ccgggacgat tgcagaggcg
      961 ctcgagcaga tgaaccgcgt gagctttgac tgcatcgtga tggacctggc cttgccggat
     1021 ggttcaggtt atgacttgct ggaacgggta tcgacgtcgc aggagcatgc gtcaccgccg
     1081 gtgattgtgt acacggggcg cgtgttgtcg ccggatgagg aacagcgcct gcgccgttat
     1141 tccaggtcga tcatcatcaa gggtgcgaag tcgccggaac ggctggtgga cgaagtgacg
     1201 ttgttcctgc acagcgtgga gagcgcgttg ccgcccgagc agcagcgcat gctgcgcgca
     1261 gcgcgccagc gcgacgacgt gttcgagggc cggacgatcc tgctggccga agacgatgtc
     1321 cgcaatatct tcgcgctgtc gcacgtgatc gagccgctgg gcgcgaagct ggaaatcgcg
     1381 cgtaatggcc gcgaggcgct cgattcgctt gcccgccatc cacagatcga tctcgtgctg
     1441 atggacgtca tgatgccgga aatggacggc ctcaccgcca tgaccgagat tcgcaagaac
     1501 cccgaccata cccggctgcc gatcattgcg ctgaccgcga aagcgatggc gagcgatcgc
     1561 cagaagtgcc tggaagcagg ggcggacgac tatatttcga agccgatcga cgtcgacaag
     1621 ctggtgtcgc tgtgtcgagt ctggttgcgc caacgatagc caggattcgc catgccacat
     1681 cgaaccctga agaacacgga agaagcggtc ttcgacatcg agttgaagct gttgctcgaa
     1741 gctgtttatc tgaagtatca gcacgacttc cgtcactact cagtgagttc gctgcgccgg
     1801 cgcctgacgc aagcgctgca cgatctgggc cttacgacgc tgtcgcaatt gcaggagcgc
     1861 attctccggg acgccacgtt gttctcgcgc ttgttccagt acctgacggt gcaggtcagc
     1921 gagatgttcc gcgacccgcc gtatttccgc gccattcgcg aacaggtcgt gccgatcctg
     1981 cagacctatc cgtccatcaa ggtgtgggtg gcgggctgca gcagcggcga ggaattgtgg
     2041 tcgctcgcca ttctgttcgc tgaagagaac attgcggacc gtacggtttt gtacgccacc
     2101 gatatcaacg ctgacgcctt gcgtcaggcc gaaaatggga tctacccgct ggatcggctg
     2161 gccagtttca gccagaatta tctggcggcg ggtggcaaac gctcgctctc cgactactac
     2221 cacgcggctt atggcgcggc gaaattttcg cagacgctca agtcgcgcgt ggtgttcgcc
     2281 gatcacagcc tggccaccga cagcgtgttt ctcgaagcgc atctcgtgtc ttgccgcaac
     2341 gtgctgatct atttcgaccg cgcgttgcag gaccgcgcgc tgggcctgtt cagcgattcg
     2401 ctggtacgac gcggatttct cgggctgggc agcaaagaga gcctgcggtt ctcaaaacac
     2461 gccggcggtt ttgccgattt cgatgcgcgc gagcgtttgt atcaaaaggt ctagcgatga
     2521 cactgtcgac tgattccctg gatgcaatag aagccgtcgt gattggggca tcggcgggag
     2581 gcgtcgaggc gttgaaccac cttctgcccg cgctgccggc caatttccgc cctgccgtgc
     2641 tggtggtcgt gcatgtcagg agtgacccgc ccagcttgct gcccgcgctg tttgccgcgc
     2701 gttgcaaact gccggtcgtc gagcctgacg acaagcacga gatcgtcggc ggcacgatct
     2761 atgttgcgcc gccggggtat cacatgctgg tcgagcccga ctgcacgatc tccctttccg
     2821 tcgatccgcc cgtgcggttt tcgaggccat cggtgaatgt tctgtttgaa tcggcggcgc
     2881 tatgttttgc cgatacgctg ctcggcatcg tgctttccgg cgccaacgac gacggcgcgc
     2941 gcgggctggt cgcgatctcg cgggcaggcg ggcgctgctg ggtgcaggac ccgcataccg
     3001 cggtggccaa cgccatgccg cttggcgcca tcgcgcgcgg cggcgtgc
//
LOCUS       DATASH010000525         6630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6809781,
            whole genome shotgun sequence.
ACCESSION   DATASH010000525 DATASH010000000
VERSION     DATASH010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6630
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6809781"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..152)
                     /locus_tag="VHV99_14500"
     CDS             complement(<1..152)
                     /locus_tag="VHV99_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX3636666.1"
                     /translation="MSAVETSKKSEVVAQFARAANDTGSPEVQVALLTTRINELTVHF
                     KAHTKD"
     gene            complement(340..1662)
                     /locus_tag="VHV99_14505"
     CDS             complement(340..1662)
                     /locus_tag="VHV99_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX3636667.1"
                     /translation="MTMAKWQRAAAAAVMALAAISTAAAARAAGEAASASASKPTSKP
                     TGAPIQLALIEGMSGPFANAGAAVERNLRFGVEQVNAQGGVQLADGAHPLELVVLDSK
                     GSTEEALVQLRAAADRHIGYVMQGNSSAVAAALIGAIDRQNSREPDNRELFLNYSADD
                     PALTNANCSFWHFRFDAHAGMRMDALVDVIQRDKAVKKVYLLNQDYSFGHDVSSLARS
                     RLATKRPDITVVGDEFHPIGRVKDFAPYIAKIRASGADAVITGNWGNDLTLLVKAARE
                     QGLGTRFYTFYGNSLGAPAALGDAGVKHVIAVADWQPNAGGPASDAWYAAFRARFPTA
                     QDDYPVLRMPLMIETLAAAMNRAGSADPLAVARALEGIKFDNGFHASWMRAEDHQLIQ
                     PLYVMEMDKAGTPGVKFDNEGSGYGFRTVLALPPERTVPSTVCKMKRP"
     gene            1847..2410
                     /locus_tag="VHV99_14510"
     CDS             1847..2410
                     /locus_tag="VHV99_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636668.1"
                     /translation="MSNRHPSTAGSIEVPPAARLRRTALICLSALTLTVTLAGCAQPW
                     QQYSQGADESTIVARLGAPRESYDLPNGGKRLMWPTQPMGETTTAADIDASGKIVTVR
                     QVLQPNEFYRAEIGKWTKSDVLVNFGRPVETSYFPLMKREVWTYRYLEDNVWYMMYSF
                     YFDDQGIVRLTQKTPDPLHDPAQRNLF"
     gene            complement(2538..4085)
                     /locus_tag="VHV99_14515"
     CDS             complement(2538..4085)
                     /locus_tag="VHV99_14515"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEX3636669.1"
                     /translation="MADKLIIFDTTLRDGEQSPGASMTKEEKIRIAKQLERMKVDVIE
                     AGFAASSNGDFDSIQTIAGLIKDSTVCSLARANDKDIQRAADALKPADHFRIHTFIAT
                     SPLHMEKKLRMTPDQVFEQAKLAVRFARKFTDDVEFSPEDGSRSDMDFLCRVLEAVIA
                     EGATTINIADTVGYGVPELYGQLVKTLRERIPNSHKAVFSVHCHNDLGMAVANSLAGV
                     QIGGARQVECTINGLGERAGNTSLEEIVMAVKTRKDYFGLDIGLDTTQIVPASKLVSQ
                     ITGFVVQPNKAVVGANAFAHASGIHQDGVLKARDTYEIMRAEDVGWSANKIVLGKLSG
                     RNAFKQRLQELGIALDSEGELNTAFARFKELADRKSEIFDEDIIAIVTEESDEAQERE
                     HYKVLSLSQHSETGEQPHAKIVFSVEGKEVTGEARGNGPVDATLNAIETEVGSGSELL
                     LYSVNAITTGTQAQGEVTVRLSKSGRIVNGVGTDPDIVAASAKAYISALNKLYSNVDK
                     LNPQRSE"
     gene            complement(4450..5319)
                     /gene="pssA"
                     /locus_tag="VHV99_14520"
     CDS             complement(4450..5319)
                     /gene="pssA"
                     /locus_tag="VHV99_14520"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEX3636670.1"
                     /translation="MAAFKPRRPRNSGQLPRPFRRNKPIVAETGAVESRRAQRQQFLR
                     KRGIYLLPNAFTTAALFCGFFAVVQAMNVRFEIAAIAIFVAMVLDGMDGRVARMTHTQ
                     SAFGEQFDSLSDMVSFGVAPALVMYEWILKDLGRWGWLAAFVYCSGAALRLARFNTNI
                     GVVDKRFFQGMPSPAAAALIAGFVWLATDNRVPLKLVWLPWVAFVLTIYAGVTMVSNA
                     PFYSGKALDVRHRVPFGVILLVVVAFVLVSSDPPLMLFGLFVLYGLSGYVFWGYQALR
                     GRANPARSVARDR"
     gene            complement(5344..5982)
                     /locus_tag="VHV99_14525"
     CDS             complement(5344..5982)
                     /locus_tag="VHV99_14525"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007588177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEX3636671.1"
                     /translation="MNYPHPIIAREGWPFIAIAAVVALLIHFFAGFGISWLFWLLLIF
                     VVQFFRDPARPIPTQANAVLCPADGRIVAVETAHDPYANREALKISVFMNVFNVHSQR
                     SPVDGAISKVEYFPGAYLNAAVDKASTENERNAVVIEMAGGQTVTSVQIAGLIARRIL
                     CYVRSGEPLTRGQRYGFIRFGSRVDVYLPVGSRPRVSIGEKVSASSTILAEL"
     gene            complement(6157..>6630)
                     /locus_tag="VHV99_14530"
     CDS             complement(6157..>6630)
                     /locus_tag="VHV99_14530"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902902.1"
                     /note="catalyzes the formation of
                     (R)-2,3-dihydroxy-3-methylbutanoate from
                     (S)-2-hydroxy-2-methyl-3-oxobutanoate in valine and
                     isoleucine biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEX3636672.1"
                     /translation="NFREETETDLFGEQAVLCGGTVDLIKAGFETLVEAGYAPEMAYF
                     ECLHELKLIVDLIYEGGIANMNYSISNNAEYGEYVTGPRIVTAETKKAMKQVLTDIQT
                     GEYAKSFIIENKAGAPTLQSRRRLTAEHQIETVGAKLRSMMPWIAANKLVDQSKN"
BASE COUNT         1285 a         2124 c         1941 g         1280 t
ORIGIN      
        1 tgatctttcg tgtgtgcctt gaagtgaacg gtcagttcgt tgatgcgggt cgtgagcagc
       61 gcgacctgaa cttcggggga gccggtgtcg ttagctgcgc gtgcgaattg cgcaacgact
      121 tctgacttct tgcttgtttc aactgcggac atgtcgattt ccttaagaac tagacaggcg
      181 gtcacggaag aaaggccgtg ccgtgggtta cccgtgtatg catggtttta gccatgcctc
      241 acaacgggac gcgtattgta gcacagccca atccggccgc gaaccctgct tggccgggcg
      301 gctcgcgggt gcccaagggt ccacttcgag ccgtttcatt caaggccgtt tcatcttgca
      361 caccgtcgac ggtaccgtac gctcgggcgg cagggccagc accgtgcgga aaccatagcc
      421 tgatccttcg ttgtcgaact tgacgcccgg tgtgccggct ttgtccatct ccatcacgta
      481 aaggggctgg atcagttgat gatcctcggc ccgcatccac gaagcgtgga agccgttgtc
      541 gaacttgatg ccctccaatg cccgggcgac ggccagcggg tcggcgctcc cggcgcggtt
      601 catcgccgcg gccagggttt caatcatcag cggcatgcgc aggaccggat aatcgtcctg
      661 ggcggtcggg aaacgggcgc gaaacgcggc gtaccaggcg tcggaggccg ggccgccggc
      721 gttcggctgc cagtcggcta ccgcgatcac gtgtttgacc ccggcgtcgc ccagcgcggc
      781 aggcgcgcca agactgttgc catagaaggt gtaaaagcgg gtgcccaggc cctgctcgcg
      841 cgccgccttg accagaagcg tcaaatcgtt gccccagttg ccggtgatga ccgcgtccgc
      901 gccgctcgcg cggatcttgg cgatgtacgg cgcgaagtcc ttcacgcggc cgatcggatg
      961 aaattcgtcg ccgacgaccg taatgtccgg acgctttgtc gccaacctcg aacgcgcgag
     1021 actgctgaca tcatggccga agctgtagtc ctggttcagg agataaacct tcttcaccgc
     1081 tttgtcgcgc tggatgacat caaccagcgc atccatacgc atgcccgcgt gcgcgtcgaa
     1141 gcggaagtgc cagaaactgc aattggcatt ggtcagcgcg ggatcgtcgg cggaataatt
     1201 gaggaacagt tcgcggttgt ccggctcgcg gctgttctgc ctgtcgatcg cgccgatcag
     1261 cgcggccgcc accgccgaac tgttgccttg catgacatag ccaatatgac ggtcggcggc
     1321 ggcgcgcagt tgcaccaacg cctcttcagt actgcccttg ctgtcgagca cgaccagttc
     1381 gagcggatgc gcgccgtcgg cgagctgcac gccgccttgc gcattcacct gttcgacgcc
     1441 gaagcgcaga ttgcgctcca ccgccgcacc cgcattcgcg aaggggccgg acatgccttc
     1501 gatcagcgcc agctggatcg gcgcacccgt gggcttgctg gtcggcttgc tcgctgaagc
     1561 ggatgccgcc tcgcccgcgg cacgagccgc ggcggccgtt gaaatcgctg ccagcgccat
     1621 gactgccgcc gctgccgccc tttgccattt cgccattgtc attgccccgc tggattcgtc
     1681 gaagcgcgga tcatagggcg ttccggacgg aataagcaag ctggcgaatc cgttaccgtc
     1741 ctttcagcaa gggcttgtac gaagcgcaaa tcttttgccg ccgcccgtgt caaaataatg
     1801 cgtctcgatg ataaaaaccg ctactcgaat gccatcggag gaagtcatgt ccaaccgcca
     1861 cccatccacg gcgggctcga tcgaagtgcc gcccgcggca cgcctgcgcc gcaccgcgct
     1921 catctgcctg agcgcgctca cgctcacggt tacgctcgcc ggctgcgcac agccctggca
     1981 gcaatacagc cagggcgcgg acgaatcgac catcgtcgcc cgcctcgggg cgccgcgtga
     2041 aagctacgat ttgcccaacg gcggcaagcg gctgatgtgg ccgacccagc cgatgggcga
     2101 aaccaccacc gccgccgaca tcgacgcatc cggcaagatc gtcactgtgc gccaggtgct
     2161 tcaacccaac gagttctatc gtgccgaaat cggcaaatgg acgaagagcg acgtgctggt
     2221 gaacttcggc cgcccggtcg aaacgtcgta cttcccgttg atgaagcgcg aggtctggac
     2281 ctatcgctat ctcgaagaca atgtctggta catgatgtac agcttctatt tcgacgacca
     2341 gggcattgtg cggctcacgc agaaaacacc tgatccgctg cacgacccgg ctcagcgcaa
     2401 cctgttctga gcttttgcgc gaccccatgc ggcgtgcgtg aacgcgccgc aaaaacacga
     2461 agccctgtac ctttcagggc acagggcttc gaactaccgc ctcgaatcag aaacgccagt
     2521 cagcgctctg cccgacatca ctccgagcgt tgcggattca acttatcgac gttcgaatac
     2581 agcttgttga gcgccgagat atacgctttc gcggaagccg cgacgatatc cggatcggtg
     2641 cccacaccgt tcacgatacg gccgctcttc gagagccgca cggtcacttc gccttgcgcc
     2701 tgcgtgccgg tggtaatcgc gttcaccgaa tacagcagca gttccgaccc gctgcccact
     2761 tccgtttcga tcgcattgag cgtggcatcc accggaccgt tgccgcgcgc ttctccggtc
     2821 acttccttgc cttcaaccga gaaaacgatt ttcgcgtgcg gctgctcgcc ggtttccgaa
     2881 tgctgcgaca gggacaggac cttgtagtgc tccctttcct gcgcttcatc cgactcctcc
     2941 gtgacgatcg caatgatgtc ctcgtcgaag atttccgact tgcggtcggc cagttcctta
     3001 aagcgcgcga acgcggtatt caattcgcct tcgctatcga gcgcgatacc caattcctgc
     3061 agacgctgct tgaatgcatt gcggcccgac aacttgccga gcacgatctt gttcgcgctc
     3121 cagcccacat cttcggcgcg catgatttca tacgtgtcac gcgctttcag cacgccgtcc
     3181 tggtgaatgc cggatgcgtg cgcaaacgcg ttcgcgccga ccacggcctt gttcggctgc
     3241 acgacgaaac cggtgatctg cgacaccagt ttcgacgccg gcacgatctg cgtggtatcg
     3301 agaccgatat cgaggccgaa gtagtccttg cgggtcttca ccgccatcac gatttcttca
     3361 agcgacgtat tacccgcgcg ctcgccgaga ccgttgatcg tgcactcgac ctgacgcgcg
     3421 ccgccgatct ggacaccggc cagcgagttc gccaccgcca tgccaaggtc gttatggcaa
     3481 tgcacagaga acacggcctt gtgcgagttc ggaatacgct cgcgcagcgt cttcacgagc
     3541 tggccgtaca gttccggcac gccataaccg accgtatccg cgatattgat ggtggtcgca
     3601 ccctctgcga tcaccgcttc cagcacgcgg cacaggaaat ccatgtccga gcggctgccg
     3661 tcttccggcg agaactcgac gtcgtccgta aacttgcggg cgaaacgcac tgccagcttt
     3721 gcctgttcga acacctggtc cggcgtcatg cgcagcttct tttccatatg cagcggcgac
     3781 gttgcaatga acgtgtggat gcggaaatga tcggccggct tgagcgcgtc agccgcgcgt
     3841 tgaatgtctt tgtcgttggc acgggccagc gagcagaccg tgctgtcctt gatcaggcca
     3901 gcgatcgtct ggatcgaatc gaaatcaccg ttcgagctag ccgcgaagcc tgcttcgatc
     3961 acgtccacct tcatccgctc gagttgcttc gcgatgcgga ttttttcttc cttcgtcatc
     4021 gacgcgccag gcgattgctc gccgtcacgc aacgtggtgt cgaatatgat cagtttgtcg
     4081 gccatgtcgg gtctcctgag gagtcgttca attgtgggga ttggatattg gaatttgggc
     4141 gttcgcgcca ccgctggcga cgctttgaat cacggacgag gcagagggag ggcggaaaac
     4201 ggtcagcgac cagcgcggcg gtgcaccagc acgagcgcgc ctggcaacgc accggcttgt
     4261 tgaggctcgt gccgtagtac aaatgaaaac gtgaacgcat tcatcggtcg actatagcgg
     4321 cattcccgct gtcgtgcaat tggcgtcaga cctgcctgcc acgccccgcc acagggcttc
     4381 ggcaaacgaa aacggcagcc cgaaggctgc cgttttcccg tgtcgctttc atgcagaagc
     4441 ggcgcgcgat tacctatccc gcgccaccga gcgagccgga ttcgccctgc cgcgcaatgc
     4501 ctggtagccc cagaacacgt agcccgacag accgtacagc acgaacaggc cgaacagcat
     4561 cagcggcgga tcggacgaca ccagcacgaa tgccaccacc accagcagaa tcacgccgaa
     4621 cggcacgcgg tgccgcacgt cgagcgcctt gccgctgtag aacggcgcgt tcgacaccat
     4681 cgtcacacca gcgtagatgg tcagcacgaa agcgacccac ggcagccaca ccagcttgag
     4741 cggcacgcgg ttatcggtgg cgagccacac gaagccggcg atcaacgcag cggcagcggg
     4801 gctcggcata ccctggaaga aacgcttgtc gaccacgccg atgttcgtgt tgaaacgcgc
     4861 gagacgcaac gccgcccccg aacagtagac aaacgccgcg agccagcccc agcgcccgag
     4921 gtccttcagg atccactcgt acatgacgag cgccggcgcc acgccgaacg acaccatgtc
     4981 cgagaggctg tcgaactgtt cgccgaaggc gctctgcgtg tgcgtcatac gcgcgacgcg
     5041 gccgtccatg ccgtcgagca ccatcgccac gaagatcgcg atcgccgcga tttcgaagcg
     5101 cacgttcatc gcctgtacca cggcgaagaa cccgcaaaac agcgcggcag tggtaaacgc
     5161 gttcggcagc aggtaaatgc cgcgtttcct caggaactgc tgacgctgag cgcgccggct
     5221 ctcgactgct cccgtctctg ccacgatcgg cttgttacgg cggaacggac gcgggagctg
     5281 tccgctgttg cgcggtcgac gcggtttgaa tgcggccatt cggaaaacct ccttggtgcc
     5341 gctttatagt tcagcgagga tcgtggacga cgcggaaacc ttctcgccga tcgacacacg
     5401 cggacggctg ccaaccggca gatacacgtc gacacgcgag ccaaaacgga taaatccata
     5461 acgctggccg cgcgtgagcg gctcacctga acgtacgtag caaagaatgc gccgcgcgat
     5521 caaacctgcg atctgcaccg acgtcaccgt ctggccgccg gccatttcga tcaccaccgc
     5581 attgcgttca ttttcggtcg aggccttatc cacggcggca ttcagatatg cgcccgggaa
     5641 atattcgact ttggagatcg cgccatccac cggcgagcgt tgcgagtgga cattgaacac
     5701 gttcatgaac acgctgatct tcagcgcttc acggttggca tacggatcat gcgcggtttc
     5761 gaccgcgaca atacggccgt cggccgggca cagcacggcg ttcgcctgag tcggaatcgg
     5821 ccgcgccgga tcgcgaaaga actgcacgac gaagatcagc agcagccaga acagccatga
     5881 aatgccgaat cccgcgaaga agtgaatcag taaagcaacg acggccgcga tggcgatgaa
     5941 cggccagcct tctcgcgcga tgatcggatg agggtaattc atggatggct tctgtatttt
     6001 tgtaaaaccg taggatagca aaagccgccc agggttcagc acccttggac ggctttttga
     6061 taagtccggc taatacgaca cggcgacgac gctgcgacga cacagcagcg aagcggcggc
     6121 gaccccgcgc cgcaccggac aaaagtaatg acttgcttag ttcttcgact gatcgaccag
     6181 cttgttcgca gcgatccacg gcatcatcga acgcagcttc gcacccaccg tttcgatctg
     6241 gtgctcagcc gtcagacggc ggcgcgattg cagcgtcggt gcgccagcct tgttttcgat
     6301 gatgaagctc ttcgcgtact cgccggtctg aatgtccgtg agaacctgct tcatcgcctt
     6361 cttcgtttca gcggtcacga tacgcggacc cgtcacgtac tcgccgtatt cggcgttgtt
     6421 cgagatcgag tagttcatgt tcgcgatgcc gccttcgtag atcaggtcga cgatcagctt
     6481 cagttcgtgc aggcactcga agtacgccat ttccggcgcg tagcccgctt ccaccagcgt
     6541 ttcgaagccg gccttgatca ggtcgaccgt accgccgcac agcacggctt gttcgccgaa
     6601 caggtcggtt tccgtttctt cgcggaagtt
//
LOCUS       DATASH010000526         1633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_480909,
            whole genome shotgun sequence.
ACCESSION   DATASH010000526 DATASH010000000
VERSION     DATASH010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480909"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VHV99_14535"
     CDS             complement(<1..227)
                     /locus_tag="VHV99_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3636673.1"
                     /translation="MTEAVKTIDVAVFGAGRIGKIHAANLACQPGVRLKYVVDVNREA
                     AAALAAEHGAQVADVDGAMGDASIGATVICS"
     gene            complement(220..1101)
                     /locus_tag="VHV99_14540"
     CDS             complement(220..1101)
                     /locus_tag="VHV99_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEX3636674.1"
                     /translation="MAEESTEDLPSVEELMQRIAENYEALPRQLKNVATYLEQHRSSV
                     MVDRTSDIAASCGVHPSAVVRFAQRFGFSGFSDLQAVFRQAYTGQGTSSPSYQQRIRK
                     LIDEKPGALSGGSVAREFIAASRGGLEELEAGLDDKQFDAAVKMLQQADNIYVIGVRR
                     SFPVASYIVYALQHTPKRVHLVSGFGGMYREQIRSVKKGDVVIAISFAPYGKETQYCL
                     RVAHHHQAKTLVITDSQLSPLARYATTQLYVKEGSAFAFRSLTSTICLCQALFIALAY
                     KLELNVEESKDTGGYDD"
     gene            1380..>1633
                     /locus_tag="VHV99_14545"
     CDS             1380..>1633
                     /locus_tag="VHV99_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006026469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX3636675.1"
                     /translation="MRLCKGKATLRILVTALTLATGFGAASAARAADAHFVLISHAPD
                     SDSWWNTIKNAIKQADEDFNVETDYRNPPNGDIADMARLI"
BASE COUNT          337 a          514 c          447 g          335 t
ORIGIN      
        1 ctcgaacaga tcaccgtcgc gccgatcgac gcgtcgccca tcgcgccgtc cacgtccgcg
       61 acctgcgcgc catgttcggc ggcgagcgcg gcagccgcct cgcgattcac gtcgaccaca
      121 tacttgagcc gcacgcccgg ctggcacgcg agattggccg catgaatctt gccgatacgt
      181 cctgcgccga ataccgccac atcgatcgtc tttaccgcct cagtcatcgt atcctcctgt
      241 gtctttggat tcttcaacgt tcagttcgag tttgtacgca agcgcgatga acaacgcctg
      301 gcacaaacag atcgtgctgg tcagcgagcg gaacgcgaat gcgctgccct ctttcacata
      361 cagttgagtg gtggcataac gcgctagcgg agaaagttgg ctatccgtaa taaccagggt
      421 cttcgcctga tgatgatgcg cgacgcgcag gcaatactgc gtttccttgc cgtacggtgc
      481 gaagctgatc gcgatcacca cgtcgccctt cttcacgctg cgaatctgtt cgcggtacat
      541 gccgccgaaa cccgacacca gatgcacgcg cttgggcgtg tgctgcaacg cgtagacgat
      601 atagctcgcc accggaaacg agcgccgcac gccgatcaca tagatgttgt ccgcctgctg
      661 aagcatcttc acagcggcgt cgaactgctt gtcgtcgaga cctgcctcga gttcttcaag
      721 accgccgcgt gaggccgcga tgaattcgcg cgctaccgaa ccaccggata acgcgccggg
      781 cttctcgtcg atcagcttgc gaatcctctg ctgataactc ggcgaggacg tgccctgccc
      841 cgtataagcc tgacgaaaca ccgcctgaag atcggagaag ccggaaaatc cgaagcgctg
      901 cgcaaaacgc actaccgcgg acggatgcac gccgcagctc gcggcgatat cgctggtgcg
      961 atccaccatc acgctcgaac gatgctgctc gagatacgtc gcgacattct tcaattgacg
     1021 cggcagcgcc tcgtaatttt ccgcgatgcg ttgcatcaat tcttcgacgc tcggcaagtc
     1081 ttcggtgctc tcttccgcca tggctctctc ttcgttgctt tattgcagtg cggaaggcgc
     1141 tttcagcgcg cgacgcaccg cctcctgacg ctgccgatta taggcaggca ccccattaaa
     1201 tggaatgtat tttccatttt tatttttcat gaaatttcca ttgcaacgct tcggaagttg
     1261 gcctaatctt ggtcgtgagc gatggtgcat cggctaaccc atgccgcaac gcacgcaacc
     1321 ggaaggccgc cccgccagac gctcccttca ataacgacag accgagaaag gtggagacaa
     1381 tgagactttg caagggcaag gctacgctca ggattctggt gacggcattg acgttggcga
     1441 cgggattcgg tgcggcgtcg gccgcccgcg cggctgacgc tcacttcgtg ctgatcagcc
     1501 acgcgccgga ttcggattcg tggtggaaca ccatcaagaa cgccatcaaa caggccgacg
     1561 aagatttcaa cgtcgagacc gattaccgta atccgccgaa cggcgacatc gccgacatgg
     1621 cgcgtctgat cga
//
LOCUS       DATASH010000527         4379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1273049,
            whole genome shotgun sequence.
ACCESSION   DATASH010000527 DATASH010000000
VERSION     DATASH010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4379
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1273049"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..175)
                     /locus_tag="VHV99_14550"
     CDS             complement(<1..175)
                     /locus_tag="VHV99_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HEX3636676.1"
                     /translation="MHPRLTLALAIMEALAIFAYAISGFIEAKTRRLDAVGTFLVAVA
                     TAFGGGTLRDVLLE"
     gene            complement(175..948)
                     /locus_tag="VHV99_14555"
     CDS             complement(175..948)
                     /locus_tag="VHV99_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEX3636677.1"
                     /translation="MPKNQPHPSEPKRARAVARPASEAAAPVVKPKIEPAAGLAGLGA
                     LRDALKGDAQRRERERAAAAAAQREASADANLFRSEIGAVAPLAVPPRATLPRNPPAP
                     LPVQTKLDEEAVLHEAISDEFDPEILLETDETLSYCRPGVSREVVRKLRRGDWIVQAQ
                     IDLHGMRREEAREALAEFIRESVKRGLRCLRVIHGKGLGSIGKEPVLKGKVRAWLVQK
                     SEVIAFCQARPHDGGAGAVVVLLQPGVLPAHARPLAEPR"
     gene            complement(1249..2247)
                     /gene="trxB"
                     /locus_tag="VHV99_14560"
     CDS             complement(1249..2247)
                     /gene="trxB"
                     /locus_tag="VHV99_14560"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX3636678.1"
                     /translation="MPATSTKHAKVLILGSGPAGYTAAVYAARANLEPVLITGLAQGG
                     QLTTTTDVENWPADANGVQGPELMQRFLEHAERFNTEIIFDHIHTARLDEKPIRLIGD
                     SGEYTCDSLIIATGASAQYLGLKSEEEFMGKGVSACATCDGFFYKQQDVAVIGGGNTA
                     VEEALYLSGIARKVTVIHRREKFRAEPILIDRLLAKEREGLVEIKWNCTLDEVVGDQS
                     GVTGLCVKNTQTGETTDIALQGIFVAIGHKPNTDIFAGQLEMKNGYIITKGGLNGFAT
                     ATSVPGVFAAGDVQDHIYRQAITSAGTGCMAALDAQRYLETLDEIGEHVMSAEADR"
     gene            2584..>4379
                     /locus_tag="VHV99_14565"
     CDS             2584..>4379
                     /locus_tag="VHV99_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEX3636679.1"
                     /translation="MAKAPYSASAQALPHRMSRLFTEIRWILQVALGVFLLMALVSYS
                     RHDPSWTHAAQVDHIANWAGRVGAWTSDILLLLFGLSAYWWIVLLGRHISANYRRITR
                     HEELQEDAPRDISWLADAFAFMLVLLACDGIEALRMWSLKVQLPRAPGGVIGEAVARG
                     VSHALGFTGGTLALLIVLGIGLSLYFRFSWLSVSERVGESIISAVTFAKLRREAGRDR
                     KLGEAAAVKREGKVEKGRVRIEEHEPVMIVPPVVTPAKSERVEKERQVPLFTDLPGDS
                     TLPPISLLDAAPAAQETISADTLEFTSRLIEKKLKDFGVDVSVVAAYPGPVVTRYEIE
                     PATGVKGSQIVGLAKDLARSLSLVSIRVVETIPGKNFMALELPNQRRQTVSLSEILGS
                     AVYADAASPLTMGLGKDIGGKPVCADLAKMPHLLVAGTTGSGKSVGINAMILSLLYKA
                     SADQVRMILIDPKMLEMSVYEGIPHLLCPVVTDMRQAGHALNWAVAEMERRYKLMSKL
                     GVRNLAGYNNKIDEAAKREEKLPNPFSLTPDDPEPLARLPNIVVVIDELADLMMVVGK
                     KVEELIARIAQKARAAGIHLILATQRPSVDVIT"
BASE COUNT          790 a         1389 c         1396 g          804 t
ORIGIN      
        1 gctcgagcag cacgtcgcgt aacgtaccgc cgccgaaggc cgtcgcgacc gccacgagga
       61 aggtgcccac ggcgtcgagc cgccgggtct tcgcttcgat aaagccggaa atcgcgtacg
      121 cgaaaatagc cagcgcctcc atgatggcca gcgccagcgt cagcctcgga tggatcaccg
      181 cggctccgcc agaggcctgg cgtgagccgg caagacgccc ggctgtaaca gcacaaccac
      241 cgcacccgcg ccgccgtcat gcggacgcgc ctgacagaag gcaatcacct cggacttctg
      301 cacgagccat gcgcgtactt tgcccttcag aacgggctct ttgccgatcg accccaggcc
      361 cttgccatga atcacgcgca aacaccgcag gccgcgcttc accgattcac gaatgaactc
      421 ggccagcgcc tcgcgtgctt cctcgcgacg catgccgtgc agatcgattt gcgcctgcac
      481 gatccaatcg ccacggcgca gttttcgcac gacctcccga ctcacgccgg gccggcaata
      541 ggagagcgtt tcgtcggttt cgagcaggat ctcgggatcg aactcgtcgg agattgcttc
      601 gtgcaacacg gcttcttcgt cgagtttggt ctgcaccggg agcggtgccg gcggattgcg
      661 cggcaaggtc gcgcgcggcg ggactgccag cggcgcaacg gcgccgatct cgctgcgaaa
      721 cagattggcg tccgccgacg cttcgcgctg cgcggccgct gccgccgcac gttcgcgctc
      781 acgccgttgc gcgtcgccct ttaacgcgtc gcgcagcgcg ccgaggccgg ccagaccggc
      841 agcgggctcg atcttcggtt tgacgacggg agcggccgcc tcggacgcgg gcctggcaac
      901 ggcacgggcg cgctttggtt cgctagggtg gggctgattc ttcggcatat cgctaataca
      961 cgcagaaaag tagatcgatc gcgtgacgct tttggggcat cggcacatgc cgcggagcgt
     1021 cgccacgaag cgccggctag aaggcgagaa gatgcaaaca ggcgcaaatg cggcgtcgac
     1081 gacgcagcgc caaagacgca gcgccaaaga cgcaagccaa acaccaagcg ccaaagacgt
     1141 aacgcaatac cgcgcttcaa taagcaaaaa agccgtcggc ttctcagccg cggccttcta
     1201 tcaagcaagg ggccgggcta tgcccagccg ccattttacc gttgcggcct agcggtcggc
     1261 ttcggcgctc atcacgtgct cgccaatctc gtcgagcgtt tccagatagc gttgcgcgtc
     1321 gagcgcggcc atacagcctg tgcccgcgct ggtgatcgcc tgacgataga tgtggtcttg
     1381 cacgtcgccc gcggcgaaca cgcccggcac gctcgtggcc gttgcgaaac cgttcaggcc
     1441 acccttggtg atgatgtagc cattcttcat ctccagctgg cctgcaaaaa tgtccgtatt
     1501 cggcttgtgg ccgatcgcaa caaagatgcc ctgcagcgca atatccgtgg tttcaccggt
     1561 ttgcgtgttc ttgacgcaca ggccggtcac gccggattga tcgccgacca cttcgtcgag
     1621 cgtgcagttc cacttgatct cgacaaggcc ttccctttcc ttcgccagca gacgatcgat
     1681 caggatcggc tcggcgcgga acttctcgcg gcgatggatc accgtcactt tcctggcgat
     1741 gccggaaaga tagagggctt cctcgactgc ggtattgccg ccgccaatca cggccacatc
     1801 ttgttgcttg tagaagaagc cgtcgcaggt agcgcaagcc gacacgccct tgcccatgaa
     1861 ctcctcttcg gatttcaggc cgagatactg cgccgacgcg ccggtcgcga tgatcagcga
     1921 gtcgcaggtg tattcgccgg agtcgccgat cagccgaatc ggcttctcat ccagcctggc
     1981 cgtgtggatg tggtcgaaaa tgatttccgt gttgaagcgc tccgcgtgct ccaggaagcg
     2041 ctgcatcagt tccggacctt gcacgccgtt tgcgtctgca ggccagtttt cgacgtcggt
     2101 ggtggtcgtc agctggccgc cctgggcgag acccgtgatg agaaccggct ccaggttggc
     2161 gcgtgccgcg tagaccgctg ccgtgtagcc ggcgggaccg gagccgagaa tcagaacctt
     2221 ggcgtgtttc gtggaagttg cgggcatgat cgaaatcctt ttacggcgcc cggctcgccc
     2281 atgcgaggcc gcgcgacttg agctgaaact atagatgggt atcagagcgg cattataaag
     2341 ggcggcacgc cggcctgctc catgaagctt tctgatcgtg gcgatagcac cgccgtgggg
     2401 cgcgggagcg tttcacgagg ccaatcggct aagttaccgt catttacagc ctcgcgttcg
     2461 acactgcgtt tacaataggc cgcatcgatg gacccggcag ccggggccgg gtctggagcc
     2521 gccctaggcg cggcttcggg ccgcgactct ggcagcgaat cggccacaca agcaacagga
     2581 tcaatggcaa aagctcccta ttccgcgagc gcgcaggcat tgccgcaccg catgtcgcgc
     2641 cttttcaccg aaatccgctg gatcctgcag gtcgcgctcg gcgtgttcct gctcatggcg
     2701 ctcgtcagct acagccggca cgatccgagc tggacgcacg ccgcgcaggt cgaccacatc
     2761 gccaactggg cgggccgtgt cggcgcgtgg acgtctgaca tcctgctgct gctgttcggc
     2821 ttgtcagcct actggtggat cgtgctgctc ggccgtcata tctccgccaa ttaccgccgt
     2881 atcacccgcc acgaggaact gcaggaagac gcgccgcgcg acatcagctg gctcgcggac
     2941 gcgttcgcgt tcatgctggt gttgcttgcc tgcgacggta tcgaggcgct gcgcatgtgg
     3001 tcgctcaaag tgcaactgcc gcgcgcgccg ggcggcgtga tcggcgaagc ggtcgcgcgc
     3061 ggggtgtcgc atgcgctggg tttcacgggc ggcacgttgg cgctgctgat cgtgctcggc
     3121 atcggtttgt cactgtattt ccgcttttcg tggctgtcgg tatcggaaag ggtcggtgaa
     3181 tcgatcattt cggccgtgac ttttgccaaa ctgcgccgcg aggccggccg cgaccgtaaa
     3241 ttgggcgaag ccgccgccgt gaagcgcgaa ggcaaggtcg aaaagggccg cgtgcggatc
     3301 gaggaacacg agccggtcat gatcgtgccg ccggtcgtca cgcccgccaa atcggagcgc
     3361 gtcgagaaag aacggcaagt gcccttgttc acggacctgc cgggcgactc caccttgccg
     3421 ccaatctcgc tgctcgacgc ggcgcccgcc gcgcaggaaa ccatttccgc cgacacgctc
     3481 gaattcacct cgcgtctgat cgagaagaag ctcaaagact tcggcgtcga tgtgagcgta
     3541 gtcgcggcct atccgggccc ggtcgtcacg cgttacgaaa tcgagccggc caccggcgtg
     3601 aagggcagtc agatcgtcgg tctggcgaag gatctggcgc gttcgctgtc gctggtgtcg
     3661 attcgcgtgg tcgaaacgat tccgggcaag aatttcatgg cgctggagtt gccgaaccag
     3721 cgccgtcaga ccgtgagtct ctcggagatt ctcggctcgg cggtttatgc ggacgcggcg
     3781 tcgccgctga ccatgggcct cggcaaggac atcggcggca agccggtgtg cgctgatctc
     3841 gcgaaaatgc cgcacttgct ggtggccggt acgaccggtt cgggcaagtc ggtgggtatc
     3901 aatgcgatga tcctgtcgct gctctacaag gcgagcgctg accaggtccg tatgatcctg
     3961 atcgatccga agatgctcga aatgagcgtc tacgagggca ttcctcatct gctgtgtccg
     4021 gtcgtcacgg acatgcgcca ggcggggcat gcgctcaact gggcggtcgc cgaaatggag
     4081 cgccgctaca agctgatgag caagctcggc gtgcgcaacc tcgccggcta caacaacaag
     4141 atcgacgaag ccgcgaagcg cgaagagaag ctgccgaatc cgttcagcct gacgccggac
     4201 gatccggaac cgctggcgcg tctgccgaac atcgtcgtcg tgatcgacga actcgccgac
     4261 ctgatgatgg tggtcggcaa gaaggtcgaa gagctgatcg cgcggatcgc gcagaaagcg
     4321 cgcgcggcgg gcatccatct gattcttgcg acgcaacgtc cgtcggtcga cgtgatcac
//
LOCUS       DATASH010000528        11874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5227392,
            whole genome shotgun sequence.
ACCESSION   DATASH010000528 DATASH010000000
VERSION     DATASH010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 11874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11874
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5227392"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /gene="gltA"
                     /locus_tag="VHV99_14570"
     CDS             complement(<1..902)
                     /gene="gltA"
                     /locus_tag="VHV99_14570"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEX3636680.1"
                     /translation="MNSKTHATLSFSDSDQTIDLPIYQGTLGPDVIDIRKLYGQTGKF
                     TYDPGFMSTAACNSEITYIDGDKGELLYRGYPIDDLAQNADFLETCYLLLKGELPNAQ
                     QKEEFVYTVTQHTMVHEQMHFFFRGFRRDAHPMAILVAAVGALSAFYHDSLDINNPQH
                     RDVSAIRMIAKLPTLVAMAYKYTVGQPFVYPKNDLSYSANFMRMMFANPAEEYEVNDV
                     LVRALDRILILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGPAHGGANEAAL
                     NMLEEIGSVDNIPEFIAKVKDKNSG"
     gene            943..1329
                     /locus_tag="VHV99_14575"
     CDS             943..1329
                     /locus_tag="VHV99_14575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636681.1"
                     /translation="MLDRPIAAWHAQAPLAFYGPQGLQLRRLTATIHLDSRNIRCLKD
                     ARNRLNQTETRRSAQRANASGALNGIASQMKVGTATATMCCGSQATQALALCDTGPVS
                     CFPCEHIAVFARRPTAGFFHQPEEQE"
     gene            1334..1735
                     /locus_tag="VHV99_14580"
     CDS             1334..1735
                     /locus_tag="VHV99_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636682.1"
                     /translation="MNASLETLFPDHVHNEDNIVTALNHQDIVVALSAALKTQKVAVL
                     HMLYPRTDARTHRSLDALVNVLHGHGLHEVAELIAQEAHYLLFKDPVKAWRAFHEIRN
                     DSLAIGVHLYYHGLVGEAAERALDKDAHRKA"
     gene            complement(1969..2754)
                     /locus_tag="VHV99_14585"
     CDS             complement(1969..2754)
                     /locus_tag="VHV99_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3636683.1"
                     /translation="MPYVEAGAGELLLFVHGSLCDYRYWQPQLAGLSKQYRCVAVSLT
                     HYWPVTDAEPDQPFSWSEHADDVAEFIDRFGAGPAHVIGHSRGGCVAFHFARRHPQSV
                     RTLTLADPGGPLQIAGRPPASLPETVNALRAKAAQLIETGEVEAGLQLFVDSVSRPGF
                     WAMSTPGFRRMATDNAHTLARQFRDPLPAYVPGQAAEVRCPVLLIDGEKSPDMFRRTA
                     TALQTWLPDARRETVRGASHGMNLAHPAAFNRYVDAFIRSVDV"
     gene            complement(3182..4234)
                     /locus_tag="VHV99_14590"
     CDS             complement(3182..4234)
                     /locus_tag="VHV99_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX3636684.1"
                     /translation="MARLTPHYPLFDRLLRHSVAVDLGTANTLIYTDNGGIVLNEPSV
                     VAFQKEAAAGHKRVAAVGAEARQLLGRAPGNVEAVRPLRYGVIANFSAAEQMIRQFVD
                     MARPRPLFSRRAAFTVCVPEGATQVERRAIKEAAVAAGAWKVSLIGESLASAVGAGLP
                     VSEAVGSMVVDIGGGTTEVGVISLGGLAYGGSIRVGGDSFDMAIVSYVRNLYGVLLGE
                     QTAEHVKKGIGTAVRDVPVEHMNATGRSVDDGLPRTVQLSNHDIADAIEGPLRQVIGA
                     VKRALETAPAELVTDIAHSGIMLTGGGALLGNLGRRLTNELGLDVHVADEPLTCAVRG
                     AGAAVAAGLLDDSAYA"
     gene            complement(4427..5086)
                     /locus_tag="VHV99_14595"
     CDS             complement(4427..5086)
                     /locus_tag="VHV99_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006046804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX3636685.1"
                     /translation="MAWAQFEHGWRRAPATLPARALVVLMHGVGSNARDLMPLADIWS
                     ESLPDVAFTSLDGTDAFDGGFGGRQWFSLRDVNQGNREARVAAAYPALRRMLEAELAY
                     WQIPFGRLALVGFSQGSIMAMHHVATSAQGAAAVVAYSGRLASVIVAQNGTPLTLVHG
                     EDDEVIPVQELEYAAAAFSNAGYTVDAYALPGIGHTIDADGVELGKQALEHALGALSR
                     D"
     gene            5398..6975
                     /locus_tag="VHV99_14600"
     CDS             5398..6975
                     /locus_tag="VHV99_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX3636686.1"
                     /translation="MSQSSSAPAGAPPAPPPPLQGGKLVLATIAVALATFMNVLDTSI
                     ANVAIPTISGNLGVSVDEGTWVITVFAAANAVSIPLTGWLTQRIGQVKLFVGAILMFV
                     LASWLCGIAPTLPILLVARIFQGAVAGPLIPLSQAILLGSYPKEKSSTALALWAMTAT
                     VGPIAGPALGGWITDSYSWSWIFYINIPVGIFAAGVTWMIYRTRESATRKPPIDVIGL
                     GLLITWVASLQIMLDKGKDLDWFASPVIVILGITALISFAFFLVWELTEENPIVDLRL
                     FQQRNFLGGTIAISVAYGVFFGNLVLLPQWMQEYLNYRSVDAGLVTAPLGIFAVLLAP
                     VMGRVLPRSDARIIATLAFIGFALVFYMRSKYVIEIDTWHLVLPTLLQGIPMALFFVP
                     LTAIILSGQPPGKIPAAAGLSNFARVFCGAVGTSIAGNEWNNRTVLHHERLTEQASVN
                     NPLFNQQIDSTQSLLHLNSQSAHALFDFTVNTQAAMMGLNDIFFVSSIIFILIIPLIW
                     ITKPAKGGGGPGAAGAH"
     gene            complement(7061..7813)
                     /locus_tag="VHV99_14605"
     CDS             complement(7061..7813)
                     /locus_tag="VHV99_14605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX3636687.1"
                     /translation="MTEAFDPDGVERPVFVVDECYHALDEPWRAYRHVRLIHVSAGVL
                     TVRTETCRWVVPSGHGLWLMANVQHCLSAAEPVRLDSLYAAPDAAPLPAQNVALALDR
                     LAEALLDAAADLPLVQPLDEPAERLLQVMLDRLSGLPTAPLALHWPRDPRIQRIAQAL
                     NANPAEPAVLDDLAASAGVTARTAARLFVKETGQTFGQWRQQLRLLVALERLGAGASV
                     TQVALEVGYSDVSSFIAVFRDTFGDTPARYFR"
     gene            complement(8098..8904)
                     /locus_tag="VHV99_14610"
     CDS             complement(8098..8904)
                     /locus_tag="VHV99_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007738215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX3636688.1"
                     /translation="MLTSLILGAIVGAVLGLTGAGGGILAVPALVAGMGWPMQQATPV
                     ALVAVAGSAAIGALEAFRQRLVRYRAALFMAAAGVPLTSLGARLAHALPQRVLLALFA
                     TVMLVVAGRLLSQALRRGRPNQLESRFCVGRINPNTGRLVWSWTTGIALAATGALTGL
                     MTGLLGVGGGFIIVPMLRKVTDVSMHGIVATSLMVIALVGSGGAVATVLHGAPLPLAL
                     TLWFGLATAAGMVAGRLASHRLSALHVQVGFGGVLICVALGMMAKAALGQ"
     gene            complement(8938..10605)
                     /locus_tag="VHV99_14615"
     CDS             complement(8938..10605)
                     /locus_tag="VHV99_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family sulfur transferase"
                     /protein_id="HEX3636689.1"
                     /translation="MDIKKLTAELAVSQQIAASDVRAIAQAGFRAVICNRPDGEGVDQ
                     PNFSEIQAAAREQGIEAHYLPIESGMVGDADAVRFGELMRTLPKPVLAYCRSGKRAAT
                     LWALSEASHRTLPDIIGTGKAAGHDLSALARRIANGGKVPVNVTVAHHDVVIVGAGAA
                     GVATAASLLARDRHLDIAIIDPAGVHYYQPGWTMVGAGVFKPEATAREMAAVLPRKVH
                     WIKAAVAAFEPEQNSVILEGCRVVSYRQLIVCPGLKLDWHAIEGLTETLGKNGVTSNY
                     RYDLAPYTWQLVQQLKGGRALFTQPPMPIKCAGAPQKAMYLASDHWRRSGQLEQIDVQ
                     FFNAGAALFGVADYVPALMEYVRRYDIALNFGFNLRAIDGPGKRAIFRRTREDGSVES
                     VSREFDMIHVVPPQKAPDFIRVSSLVDKAGWVDVDPATLRHKTYSNVFALGDVANTTN
                     AKTAAAARKQAPVVAHNVLAALGKDKGVAVYDGYGSCPLTVERGKIVLAEFLYGGKVA
                     PSMPTWLIDGKRPSRLAWLLKARILPPVYWHGMLKGREWLVQPELVD"
     gene            complement(10645..11532)
                     /locus_tag="VHV99_14620"
     CDS             complement(10645..11532)
                     /locus_tag="VHV99_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007738217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX3636690.1"
                     /translation="MLNTTSPMQVEGFFEPGTSTISYLLLDVRTGDCALIDSVLDYDP
                     KSGRTSTTTADKLIARVQELGARVEWLLETHVHADHLSAAPYLKERLGGKIAIGAQVT
                     RVQDVFGKLFNAGMKFADDGSQFDQLLSDGEAFTVGGLQARAIHTPGHTPACMTYVVS
                     DGTRTAAFVGDTLFMPDYGTARCDFPGGDARTLFRSINKVLSLPADTQLYLCHDYQPG
                     GRPLQFVTTVAEQRAHNIHIRDGINEEAFVAMRTTRDATLNMPVLMLPSVQVNMRAGH
                     LPDPEDNGVLYLKIPLNAI"
BASE COUNT         2209 a         3814 c         3622 g         2229 t
ORIGIN      
        1 cctgagttct tgtccttcac cttcgcaatg aactcaggaa tgttgtcgac cgaaccaatc
       61 tcttccagca tattcaatgc ggcttcattc gcgccgccat gcgccggacc ccacaaacac
      121 gcaatgcccg ccgcaatgca ggcaaacgga ttcgcacccg acgaaccggc taaccgaacc
      181 gtcgaggtcg acgcattctg ctcgtgatcc gcatgcagga tcagaatacg atccagcgcg
      241 cgcaccagca cgtcattgac ctcgtactct tccgccggat tggcgaacat catccgcatg
      301 aaattcgcgc tgtacgacaa gtcgttcttc ggataaacaa acggctgccc cacggtgtac
      361 ttgtacgcca tcgccaccag ggtcggcagc ttcgcgatca tgcgaatcgc ggatacgtcg
      421 cgatgctgcg gattgttgat gtcaagcgag tcatgataga acgccgacaa cgcgccgacg
      481 gccgccacca gaatcgccat cgggtgcgca tcacggcgga atccgcggaa aaagaagtgc
      541 atctgctcat gcaccatcgt gtgctgcgtc acggtgtaga cgaactcttc cttctgctgc
      601 gcattcggca attcgccctt cagcaacaga taacacgtct cgaggaagtc ggcgttttgc
      661 gccagatcgt caatcggata accgcgatac agcagctcgc ctttgtcacc gtcgatatac
      721 gtgatttcag aattgcatgc cgccgtggac atgaaacccg ggtcgtacgt gaacttgccg
      781 gtctggccgt acagcttgcg aatgtcgatg acatccggcc cgagcgtgcc ctgataaatg
      841 ggcaaatcaa tcgtctggtc gctgtcggaa aagctcagcg ttgcgtgtgt cttcgaattc
      901 atctgcacgt tccttttact ttcacatcgc acacatgccg gcatgctcga tcgtccgatc
      961 gccgcgtggc atgctcaggc tccgctcgca ttttacggtc cgcaagggct tcaattgcga
     1021 cgattgaccg caacaatcca tctcgactcg cgcaacattc gttgtctgaa agacgcgcgt
     1081 aatcgcctga accagacaga aacacgcaga agtgcgcagc gcgcaaacgc gtccggtgcg
     1141 ctcaacggca ttgccagcca aatgaaagtt ggtacggcta ctgcaacaat gtgttgcggc
     1201 agtcaagcta cacaagcgct agcgctctgc gatactggac ccgtgtcttg cttcccctgc
     1261 gaacacatcg ctgtatttgc ccgccgtcca acggcgggct tttttcatca gccagaagaa
     1321 caggaatgac cagatgaacg cctctctcga aacgctgttt ccggatcacg tccacaacga
     1381 agacaacatc gtcaccgcgc tgaatcatca ggacatcgtc gtggcgttgt cggcggcgct
     1441 aaagacgcaa aaggtggcgg tgctgcatat gctttatccg cgtaccgatg cgcgtaccca
     1501 ccgcagtctc gatgccctcg tgaacgtgct gcacggccat ggcctgcatg aagtcgcgga
     1561 actgatcgcg caggaggcgc actatctcct cttcaaagac cctgtgaagg catggagagc
     1621 cttccacgaa attcgcaacg actcgctcgc gatcggcgtg catctgtatt accacggtct
     1681 cgtcggcgaa gccgcggaac gcgcgctcga taaagacgcg catcgcaagg cttgaactgc
     1741 aaccgttgag caagtgctga atgttgagtt cggggtgctg aattcggggt gctgagttcg
     1801 gggtgctgaa ttcgaggtgc tgggcgcctc gcgctcgaat cgctgcgagg caagtagcat
     1861 cagctcatcg cgggttgatt gcaggctgtt cgtccactcg ttgtccattc ggtggcgcgc
     1921 tccagtgcgc gaatcaagca cccaatgtcc actcatgtca cgcgtttatc aaacgtccac
     1981 tgagcgaatg aacgcgtcga cgtagcgatt gaacgccgcc ggatgcgcga ggttcatccc
     2041 gtgcgaggcg ccgcgtaccg tttcgcggcg cgcatccggc agccatgttt gcagcgccgt
     2101 cgcagtgcgg cggaacatgt cgggactctt ttcgccatcg atcagcagca ccgggcagcg
     2161 cacttcggcc gcttgcccgg gtacataagc gggcaacgga tcgcggaatt gacgcgcgag
     2221 cgtgtgcgcg ttgtcggtcg ccatcctgcg aaaaccgggc gtgctcattg cccagaaacc
     2281 gggccggctc acggaatcga cgaatagttg caagccggct tccacttcgc ctgtttcgat
     2341 cagttgggcg gctttcgcgc gcaacgcatt gacggtctcc ggcaaactcg cgggcggtcg
     2401 cccggcgatc tgcaagggac cgcccggatc ggcgagcgtc agcgtacgca cggactgtgg
     2461 atggcgtcgc gcgaaatgaa acgccacgca gccgccacgc gaatggccta tcacgtgcgc
     2521 cggtccggcg ccaaaacgat cgatgaattc ggccacgtcg tccgcatgtt cgctccagct
     2581 gaacggctga tcgggctcgg catccgtcac tggccagtaa tgcgtgagac tcaccgcgac
     2641 acagcggtac tgcttcgaca gacccgcgag ttgcggttgc caataacggt aatcgcacag
     2701 cgagccgtgc acgaacagca gcagttcgcc agcgcccgct tccacataag gcatacgcaa
     2761 gccgctatgc agctcgataa aagacgaaga tgcggacgag gacgaagcgg aaacagattg
     2821 attcacggat ggttcacgga cggcgcggca acgcctgata cggcgacgct ggctggaaat
     2881 ggattcgagg aagaaaacgt gtttgcgtcc ctgacatcgg gattcgagtc gtattgtcac
     2941 acggctgtta aatatgcgcg gaggcttgtt cggctgcgtg cagtggcgtg aatctggtga
     3001 tgtgaatctg gcggcttgca cgtagtgact tgcgccgaac gcctgggcat ctgcgcatca
     3061 tgcccagaaa cccacgcggc tgacctcgcg gccagtgcat cgtgcccgtg ggcccacgcg
     3121 gtcacccccc gcctcggcca ccggccaccg gccacctgcc acgcggacgt cccgcgccga
     3181 atcacgcgta ggcgctgtcg tcgagcaaac cggcggcaac cgccgcgccc gcgccacgca
     3241 ccgcgcaggt gagcggctcg tcggcgacgt ggacgtcgag cccgagttcg ttagtcaggc
     3301 gccggcccag attgccgagc agcgcgccgc cgccggtcag cataatgccg ctgtgcgcga
     3361 tatccgtcac cagttcggca ggcgccgttt caagcgcgcg tttcacggcg ccgatcacct
     3421 gacgcagcgg gccctcgatc gcatcggcga tatcgtggtt gctcaattgg accgtgcgcg
     3481 gcaagccgtc gtcgacgctg cgcccggtcg cgttcatgtg ctcgaccggc acgtcgcgca
     3541 cagccgtacc gatgcccttc ttcacgtgtt cggcggtttg ctcgccgagc agcacgccat
     3601 acaggttgcg cacataactg acgatcgcca tatcgaaact gtcgccgccc acgcgaatcg
     3661 agccgccata cgcgaggccg cctagcgaga tcacaccgac ttcggtcgtg ccgccgccga
     3721 tgtcgaccac catcgagccg accgcctccg acaccggcag gcccgcaccg accgccgacg
     3781 ccagcgattc gccgatcaga ctgaccttcc acgcaccggc cgccaccgcg gcttccttga
     3841 tcgcgcgacg ctcgacctgg gtcgcgccct ccggcacgca cacggtgaat gcggcgcgcc
     3901 ggctgaacag cggccgcggg cgggccatat cgacgaattg ccggatcatc tgctcagcgg
     3961 cggaaaaatt ggcgatcacg ccatagcgca gcggccgcac ggcttcgacg ttgcctggcg
     4021 cgcggccgag cagctgacgc gcttcggcgc ccactgccgc gacgcgtttg tgcccggcgg
     4081 cggcctcttt ctggaacgcc acgacggacg gctcgttcag cacgatgccg ccgttgtcgg
     4141 tatagatgag ggtattggcc gtaccaaggt caactgccac ggaatgacgg agcagtcgat
     4201 cgaaaagcgg gtaatgcggc gtcaatctgg ccataggaag gctctgatat gtcgttatcc
     4261 gcccttgccg ggcagtctgg aaaatgcgcc cccggtcacc ccgtgccgcc tggtacgccc
     4321 ggatgggcat tgcgcgttcc gcgcggcgga ggtgattttc atactaatga tcagataacg
     4381 gcaagcaaaa cagaatcttt agggccactc ttcaatttca ggcgggtcag tcgcgtgaca
     4441 aggcgcccag cgcatgctcc aacgcctgct tgccgagttc gacgccgtcc gcgtcaatcg
     4501 tgtgcccaat cccgggcaag gcgtaggcat cgaccgtgta gccggcgttg ctgaatgcag
     4561 ccgcggcgta ttccagttcc tgcaccggga tcacttcgtc gtcttcaccg tgcacgagcg
     4621 tgagcggtgt gccgttctgc gcgacgatca ccgaggcgag ccgccccgaa tacgcgacca
     4681 ccgcggcagc gccttgcgcg ctggttgcca cgtgatgcat cgccatgatc gagccttgcg
     4741 agaaacccac cagtgcaagc cggccgaaag gaatctgcca gtacgccagt tccgcttcga
     4801 gcatgcgccg caaggccgga taggcagcgg cgacacgcgc ctcgcggtta ccttggttga
     4861 catcgcgcag gctgaaccat tggcggccgc caaagccgcc gtcgaaagcg tcggtgccgt
     4921 cgagcgaagt gaacgccacg tccggcagac tttcgctcca gatgtccgcg agcggcatca
     4981 gatcgcgcgc attgctgccc acgccgtgca tcagcaccac caatgccctg gcgggcaggg
     5041 tcgccggcgc acgccgccag ccatgttcga attgcgccca ggccatcttc aactcctctt
     5101 tgtgttcgct gtattgccgg ttttgccggt tcgtgtttcc gcatgcatag ccaggcactc
     5161 gtcaacgtgc tgccgtcttg cacgcgcacg ccgactcgcg aaactccatg cagtctcagc
     5221 cgggagcata cgctgccgga ctggtttgcg ccgtcggacc tgcgtgggtc gacgacgcca
     5281 aaacccgaat acaggtatgc ttttgcgaga attcgccggg atgttctatt cactgcccgg
     5341 gcagtaaata gaacacctgc atagaacaat ccgtcgcacc ggaatccgga gaaccgtttg
     5401 agccagtcct cttccgcgcc cgctggcgcg ccgcccgccc caccgccgcc gctgcaaggc
     5461 ggcaaactgg ttctggcgac catcgccgtc gcgcttgcca ctttcatgaa cgtgctcgac
     5521 acgtcgattg cgaacgttgc gattccaacc atttcgggca acctgggcgt ctcggtcgac
     5581 gaaggcacgt gggtcatcac cgtgttcgcc gcggccaacg ccgtctcgat tccgctgacc
     5641 ggctggctca cgcaacgcat cggccaggtc aagctgttcg tcggcgcgat tctcatgttc
     5701 gtgctggctt cgtggctatg cggcattgcg cccacgctgc cgatcctgct ggtcgcgcgg
     5761 attttccagg gcgcggtggc gggtccgctg attccactct cgcaagcgat tctgctcggc
     5821 tcgtatccca aggagaaatc ctcgaccgcg ctcgcgctat gggcgatgac ggccaccgtc
     5881 ggcccgattg cgggccccgc gctcggcggc tggatcacgg atagctacag ttggtcgtgg
     5941 atcttctaca tcaacatccc ggtcggtatt ttcgcggccg gcgtcacgtg gatgatctat
     6001 cgcacccgtg aatcggcaac ccgcaagccc cccatcgacg tgatcgggct cggcctgctg
     6061 attacgtggg tcgcgtcgct gcagatcatg ctcgacaagg gcaaggacct cgactggttc
     6121 gcgtcgccgg tgatcgtgat tctcggcatt accgcactga tcagcttcgc tttcttcctc
     6181 gtgtgggagt tgaccgaaga gaatccgatt gtcgatctgc ggctcttcca gcagcgcaac
     6241 tttctcggcg gcacgattgc gatttcagtg gcgtacggcg tgttcttcgg caatctggtg
     6301 ctgctgccgc aatggatgca ggagtatctg aactaccgct ccgtggatgc cggtctggtt
     6361 accgcgccgc tcggcatctt cgcggtgctg cttgcgccgg tgatgggccg cgtgttgccg
     6421 cgctcggatg cacgcatcat cgccaccctc gcgtttatcg gctttgcgct cgtgttttat
     6481 atgcgctcga agtacgtgat cgaaatcgat acctggcacc tcgtgctccc cacgctgctg
     6541 caaggcatcc caatggcgct tttcttcgtg ccgctaactg cgatcatttt gtccggtcag
     6601 ccaccaggca aaattcctgc agccgcgggc ctgtcgaatt tcgcgcgggt attttgcggc
     6661 gcggtgggca cctcgatcgc cggcaacgag tggaataacc gcacggtgct gcaccatgaa
     6721 cggctcaccg agcaggccag cgtcaacaat ccattgttca accagcagat cgattcgacc
     6781 cagtcgctgc tgcatctgaa ttcgcaatcg gcgcatgcgc tgttcgattt caccgtgaac
     6841 acccaggccg cgatgatggg cctgaacgac attttctttg tgtcgtcgat cattttcatt
     6901 ctgatcattc cactgatctg gattacgaag ccggccaagg gtggcggcgg cccaggtgcg
     6961 gcgggcgcgc actgattgcg gtgttttcag tccccgcgat tccaggctac gacgacggat
     7021 cgcggcgatt ccagtcgatg aagacgcaaa gggcgcgggg tcagcggaaa tagcgtgccg
     7081 gcgtgtcgcc aaaggtgtcg cggaacacgg cgataaatga cgatacgtcg ctgtagccga
     7141 cctcaagtgc cacctgcgtg acgctcgccc cggcgcctag acgctccagc gcaaccagca
     7201 gccgcagttg ctgccgccac tggccgaagg tctgcccggt ctccttgacg aagaggcgcg
     7261 ccgctgtgcg cgcggtcacg ccggcgctcg ccgccagatc gtcgagtacc gcgggctccg
     7321 ctgggttggc gttcagcgcc tgggcaatcc gctggatgcg cggatcgcgc ggccagtgca
     7381 gcgccagggg cgccgttggc aagccggaca ggcggtccag catgacttgt agaaggcgct
     7441 cagcgggttc gtcgagcggt tggacgagcg gcagatcggc cgcggcatcc agcaaagcct
     7501 ctgccagccg gtcgagcgcc agcgcgacgt tttgcgcggg gagtggcgcg gcgtccgggg
     7561 cggcatacag cgaatcgagc cgcacaggct cggcagcaga caggcaatgc tgcacgttcg
     7621 ccatcagcca gagcccatga cccgacggca cgacccatcg gcaggtttcg gtgcgcacgg
     7681 tcagcacgcc ggcgctcacg tggatcaagc gcacgtgacg ataggcgcgc cacggctcgt
     7741 ccagcgcgtg gtaacactcg tcgaccacga ataccggacg ctcgacgccg tcgggatcga
     7801 aggcttccgt catgtacgtc tgttttgccc ggttgcgatc agaaatgcag caagattagc
     7861 ctagtccttg tgagttgctc cttcaacgca gcagcgtctc gccgcacgaa caattagcgc
     7921 aaggttgcca gtagaatcgc cgcttcagtg gaggttggcg cagattgccg gcaccttgca
     7981 atttcaatga acagcggcga gctggcgaag gcggggctgt ttctgaccaa tcatggttgt
     8041 gcccgccact ggcgagcgga ttcccgacac gagctcggcc aaacggctcc gcccgggtca
     8101 ttgacccagt gcggcttttg ccatcatgcc gagcgccaca cagatcaaca cgcctccgaa
     8161 acccacctgg acgtgcagcg cggaaagacg gtgcgaggcc agccgcccgg ccaccatgcc
     8221 agccgccgtc gcgagaccga accacagcgt cagcgcgagc ggcagcggtg caccatgcag
     8281 caccgtcgcg accgcaccac cgctgccgac cagcgcgatc accatcagcg acgtggcgac
     8341 gatgccgtgc atcgacacat cggtcacttt gcgcagcatc ggcacgatga tgaagccccc
     8401 gcccacaccg agaagtcccg tcatcagccc ggtcaacgcg ccggttgccg cgagtgcaat
     8461 cccggtggtc cacgaccaga ccagccgccc ggtgttcggg ttgatgcggc caacgcaaaa
     8521 tcgagactcg agctggtttg ggcgcccacg ccgcagcgcc tgtgatagca gtcttcccgc
     8581 caccaccagc atgacggtcg caaacagcgc caatagcacg cgttgtggca acgcgtgcgc
     8641 aagacgcgca ccgagtgagg tcagcggcac gcccgctgcg gccatgaaaa gcgcggcacg
     8701 gtagcggacg agccgctgcc ggaacgcttc aagcgcgcct atcgcagcgc tgcccgccac
     8761 ggcgaccagc gcgaccggcg tcgcctgctg cattggccag cccatgccgg ccaccagcgc
     8821 cggaacggcc aggatgccgc cgcccgcgcc ggtcaggccg agcaccgcgc caacgatggc
     8881 gccgagaata agtgaagtca gcatgtcgaa tccgaaaggt tgccagtagc caccgcgtta
     8941 atccacgagc tcaggctgca ccagccactc gcgccccttg agcatcccgt gccagtaaac
     9001 cggtggcagg atgcgcgcct tcagcaacca cgcgagtcgc gacgggcgct tgccgtcgat
     9061 cagccaggtc ggcatggacg gtgcgacctt tccgccatag aggaattccg cgagcacgat
     9121 cttgccgcgt tcgacggtca gcggacatga gccgtagccg tcatacaccg ccacgccctt
     9181 atctttcccg agggcggcaa gcacgttatg ggcgacgacg ggagcctgct tgcgtgcggc
     9241 ggcagcggtc ttcgcattgg ttgtgttcgc gacgtcgcca agcgcgaaga cgtttgagta
     9301 ggtcttgtgg cgcaacgtcg ccgggtcgac gtcgacccaa cccgccttat cgaccagaga
     9361 gctgacgcga atgaagtcgg gtgctttctg cggcggcacc acatggatca tgtcgaactc
     9421 gcggctgacc gattccacgc tgccgtcctc gcgcgtgcgc ctgaaaatcg cgcgcttgcc
     9481 cggaccgtcg atcgcgcgca ggttgaagcc gaagttcaga gcgatgtcgt aacgcctgac
     9541 atattccatc agggccggta catagtcggc cacgccgaac aacgcggccc ccgcattgaa
     9601 gaactggacg tcgatctgct cgagctggcc tgaacgccgc cagtgatccg aggcgagata
     9661 catcgctttc tggggcgccc cggcgcattt gatcggcatc ggcggttgcg tgaatagcgc
     9721 gcggccaccc ttgagctgct gcacgagctg ccacgtatag ggcgcgagat cgtaacgata
     9781 attggaggtc acgccgttct tgccgagggt ctcggtcagg ccttcaatgg catgccagtc
     9841 cagcttgagc cccgggcaca cgatgagctg cctgtagctc accacccggc atccttcgag
     9901 gatgaccgaa ttctgttccg gctcgaacgc ggccactgca gccttgatcc agtgcacctt
     9961 gcgcggcagc acggcggcca tctcgcgggc ggtggcttcg ggcttgaaca cccctgcacc
    10021 gaccatggtc cagcctggct ggtaatagtg aacgccggcc gggtcgatga tggcgatgtc
    10081 gaggtggcgg tcgcgcgcga gcaggctcgc ggcggtcgcc acgccggcgg ctcctgcgcc
    10141 aacgatcacg acgtcgtgat gggccacggt gacgttgacg ggcaccttgc cgccgttggc
    10201 gatacgccgc gccagcgcgg acagatcatg acccgcagcc ttgccggtcc cgatgatgtc
    10261 cggcagcgtc cggtggcttg cttcagacag tgcccacaaa gtcgcagccc gcttcccgga
    10321 gcggcaataa gcaagcaccg gtttcggcag cgtgcgcatc aattcgccga accgcaccgc
    10381 atcggcgtca ccgaccatgc cggactcgat gggcaggtaa tgagcctcga tgccctgttc
    10441 ccgggccgcc gcctggatct cgctgaagtt gggctggtcg acgccttcgc cgtccgggcg
    10501 attgcagatg acggcgcgaa agccggcttg cgcgattgcc cgcacgtcgg atgcggcgat
    10561 ctgttgcgat accgccagct cagccgttag tttcttgatg tccatcgttt tttccagttg
    10621 cttctatcag ttgggggcac cggctcagat cgcgttgagc ggaatcttca gatacagcac
    10681 gccgttgtct tccggatcgg gcagatgtcc ggcgcgcatg ttgacctgca ccgacggcag
    10741 catcagcacg ggcatgttga gtgtcgcgtc gcgcgtggtg cgcatcgcga cgaaggcctc
    10801 ctcgttaata ccgtcgcgga tatggatgtt gtgcgcgcgt tgctcggcga cggtcgtgac
    10861 gaactgcaac ggccggcctc cgggttggta gtcgtgacac aggtacagct gggtgtcggc
    10921 cggcaagctc agcaccttgt tgatcgaccg gaaaagcgtg cgtgcatcgc cgccgggaaa
    10981 gtcgcagcgc gccgtgccat agtcgggcat gaacagggtg tccccgacaa aggccgcagt
    11041 ccgcgtgccg tcgctgacga cataggtcat gcaggcgggc gtatggcccg gggtatgaat
    11101 agcgcgcgcc tgcaagcctc cgacggtgaa cgcttcgccg tcggagagca gctgatcgaa
    11161 ctggctgcca tcgtcggcga atttcatgcc ggcgttgaag agcttgccga acacgtcctg
    11221 cacgcgcgtc acctgcgcgc cgatcgcgat cttgccgccg agccgttcct tgaggtacgg
    11281 cgcagcggag agatggtcgg cgtgcacgtg ggtttcgagc agccactcga ccctggcgcc
    11341 aagttcctgg acgcgggcga tgagcttgtc ggctgtcgtc gtggaggtgc gacctgactt
    11401 cgggtcatag tcaaggacgc tgtcgatcag tgcacaatcg cctgtgcgga catccagcaa
    11461 caggtaacta atcgtcgagg taccgggctc gaagaagcct tcgacctgca tggggctcgt
    11521 ggtattcaac atcctctcct taatcaaagg gtgctttgcg gtaagtaggc tggtccgtta
    11581 gtacaagaac cgtgccatcg catctccctt ttgatttcgg atccgtagcc atttgatttt
    11641 tatcaatatt ttttgttgcg tcggttgacg gagcatctct tcagagcgct aaccagagcg
    11701 ccgatcagcg ctaagactgc cagttctggc agtcaggtgg cagtcaggtg gcagttgtgg
    11761 cagtatcgac gcttcgtgct tactgaagcc accgccgtga aaccctcaaa accggcgata
    11821 tccgtcattc ccgtcgtaac gacctcggct gaaccgccac aggcgccgga tgtc
//
LOCUS       DATASH010000529         5274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_481288,
            whole genome shotgun sequence.
ACCESSION   DATASH010000529 DATASH010000000
VERSION     DATASH010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5274
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481288"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="VHV99_14625"
     CDS             complement(<1..727)
                     /locus_tag="VHV99_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007739039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX3636691.1"
                     /translation="MSRPPDNRSPDIPDAVAMPRKRWRMQLVWLVPIVAVLIGGWLAV
                     KAVLDKGPTITISFATGEGLEAGKTKLKFKNVDIGTVTSVVLSPDHRRVIARAELAKD
                     AASLLVDDTRFWVVRPRISGGTVSGIGTLLSGSFIGVDVGTAKTLRREFVGLETPPVI
                     ATGVPGREFILKGVDMGSLDVGSPIFFRRLQVGQIASYELDANGKGVTLHVFVNAPYD
                     KYVKDDTRFWHASGIDVALDTNGV"
     gene            complement(708..1985)
                     /locus_tag="VHV99_14630"
     CDS             complement(708..1985)
                     /locus_tag="VHV99_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008644312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEX3636692.1"
                     /translation="MTTGRLIACHECDLLQRELPLPLNGVLRCRRCNAELSRSRPDSV
                     DRALAFTLSAMVLFAIGNAFPMVGLDVNGELVQTTLLGAARVLYDDGMWPIAGLVMAT
                     TFVMPLLQMATMTYLLVPLRFGRVPYHFDVIFRLLKLVRPWGMTEVLILGMLVALVKL
                     AHIASVVPGTALWTFGALMVLLAAASSAFDPREFWERTRVTQAAGPEIVDSDTPRART
                     AMGCGLLTCHTCEQLVKPAAHSADAACPRCGAHLHARKPNSIGRTWALLIAAMVLYIP
                     ANALPVMNTSSLFGSEKDTILSGVVYLWNSGSWPLAVIVFIASIAVPMLKIIALVFLV
                     VSTQIHSAWSPQQRARIYRVVELVGRWSMLDIYVITILVALVQFKGLATIQAGPAAIA
                     FGSVVVLTMFAALSFDPRLIWDWAENNHVPAAR"
     gene            complement(1982..3466)
                     /locus_tag="VHV99_14635"
     CDS             complement(1982..3466)
                     /locus_tag="VHV99_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008348477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEX3636693.1"
                     /translation="MRSRASLPLLVPVLLSLGGCLLGPNYSRPAIDTPATYRFAAGET
                     VEVANTAWWEQFQDPVLNDLIATALAENKDVKIAAARVDQFLGQFVTTRAGLFPQMSA
                     GFDAQRQGVSTAGAVPLPAGLSPVFNIFEPALSAAWEIDVFGRVRRQTEAARAGLLAS
                     EEGRRATILTLVASVATAYINLRDLDQQLEIAKSTTESRAESVHVFTLRFKGGDVSQM
                     ELAQSQSEYEASLATIPQLQTQIAQQENALSILLGRNPGDIVRGRELSDLAVPAVPAG
                     LPSDLLTRRPDLLQAEQNLVAANALIGAARALYFPSISLTGLFGSASVEFSKLFTGPA
                     RVWSFAGSVSVPIFTAGSISGQVRQAEAQQQQALFQYQQAIQTAFQEVDDALISVKNS
                     REQLVVQERQVDALRTYARLARLRFEGGYTSYIEVLDAERSLFNAQLSYTQTNGAVFS
                     SLVALYKAMGGGWVVTAEQMTHTTPQQEGSVVQKTGEAHAELPR"
     gene            complement(3468..>5274)
                     /locus_tag="VHV99_14640"
     CDS             complement(3468..>5274)
                     /locus_tag="VHV99_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX3636694.1"
                     /translation="ITIAISVVLSGVVALTLSPALAALLLKPGHHEKRGFFRWFDNAF
                     ARMTTGYANLVKLAIKRSVIGLLIFVGMIVLSVVMMRSIPTSFLPPEDQGYLLGAVIM
                     PDAASLDRTEAVTQHVTDYFMKQPAVGSVTIVNGFSLLDSQNKNNAGTFFIGFKSFDE
                     RYKFANIKTQNARAVLIDAYENLSAVQEGIILPVNPPSIPGLGTTGGTEVWVQAQGDA
                     TTAQLAGVVDQFVAKAKARPELARVTSTFNASAQQLLVNVDRDKSETLGVPIEDVYSA
                     MQTMFGSLYVSQFNRSSRLWQVILQAEPSYRLKPQDLDQIFVRSKTNSMVPLKTLVTS
                     KFDTGPDLVTRFNNFPAVKVTVNPAPGISSGQVLNTLDEVGAQVLPDGYSLGWSGEAY
                     EARKAGGTSALVFVFGLIMVFLILAAQYEKWSLPFGVLMAVPFALFGALLAIMLRGLE
                     NDVYFQIGLTMLVALAAKNAILIFEFAVINRESGESVYDSAVSAATERLRPIVMTSLA
                     FILGCVPLAIALGASANSRHSIGTGVIGGMLGATAIAVFFIPMFFYLLESMSEKSGGK
                     KKPGPTASKPGDDAEGGTGAGGAPTITPSARHEGE"
BASE COUNT         1030 a         1722 c         1636 g          886 t
ORIGIN      
        1 tgacgccatt ggtgtcgagc gccacgtcaa tgccgctggc gtgccagaag cgggtatcgt
       61 ccttgacata cttgtcatag ggcgcattga cgaacacatg cagggtcacc cctttgccat
      121 ttgcatccag ctcgtacgac gcgatctgtc caacctgcag gcgcctgaaa aagatcggag
      181 agcccacatc gagcgagccc atgtccacac ccttcaggat gaattcgcgc cccggcacac
      241 ccgtggcaat cacaggcggc gtttcgagac cgacaaactc acggcgcaac gttttcgcgg
      301 tgcccacatc cacgccaatg aaagaaccgg ataacaacgt gccgatgccc gacacggtgc
      361 cgccggaaat gcgcgggcgc accacccaga agcgcgtgtc gtcgaccagc aggctcgcag
      421 cgtccttggc gagttccgcg cgcgcaatca cgcggcggtg gtccggcgac agcaccacac
      481 tcgtgacagt accgatgtcg acgttcttga acttgagctt ggtcttgccc gcttcgagac
      541 cttcgccggt cgcaaagctg atggttatcg tcggcccctt gtcgagcacc gccttgacgg
      601 caagccagcc gccgatcagc acagcgacga tcggcaccag ccagaccagt tgcatacgcc
      661 agcgcttgcg cggcatggcc acggcatcgg gaatgtcggg cgaacggtta tcgggcggcc
      721 gggacatgat tattctccgc ccagtcccag atcaggcgcg ggtcgaacga tagtgcggca
      781 aacatcgtga gaacgacgac cgacccgaaa gcaatagcgg ccgggccggc ctggatggtc
      841 gcaagcccct tgaactgcac gagcgcgacg agaatagtga tgacgtagat gtcgagcatc
      901 gaccagcgcc cgaccagttc caccacccgg tagatccgcg cccgttgttg cggcgaccag
      961 gccgagtgga tctgcgtgga gacaacaaga aataccaacg cgatgatctt gagcatcggc
     1021 acggcgatgc tcgcaataaa cacaatgacg gcgagcggcc atgagccgga gttccagaga
     1081 tagaccacac cgctcaggat cgtatctttc tcgctgccga acagcgaact ggtattcatg
     1141 acgggcaacg cgttcgccgg aatgtacagg accatcgcgg cgatcaggag cgcccatgtg
     1201 cggccgatac tgttgggctt gcgtgcgtgc agatgcgcgc cgcagcgcgg gcaggcagcg
     1261 tcagcgctgt gcgcggcggg tttcacgagc tgttcgcacg tgtgacaggt cagcagcccg
     1321 caacccatgg ctgtgcgcgc gcgtggggtg tctgaatcga cgatctccgg gccggccgcc
     1381 tgcgtgacgc gcgtacgctc ccagaattca cgtggatcga acgcactgct cgccgcggca
     1441 agcagcacca tgagtgcgcc gaacgtccac aacgccgtgc cgggtacgac gctggcgatg
     1501 tgggccagct tgaccaatgc gaccagcatg ccgagtatca ggacctcggt catcccccaa
     1561 ggacgaacca gtttcagaag acggaagatc acgtcgaagt ggtacggcac ccggccgaag
     1621 cgaagcggca ccagcagata agtcatggtc gccatctgca ggagaggcat cacgaatgtc
     1681 gtcgccatca cgagcccggc gatgggccac ataccatcgt cgtacagcac gcgggcggcg
     1741 cccagcaagg tcgtctgaac gagctcgccg ttgacatcga ggccgaccat cggaaacgcg
     1801 ttcccgatgg cgaacagcac catcgcgctg agcgtgaacg ccagcgcccg gtcgacgctg
     1861 tccggacggc tgcgcgacaa ctcggcgttg cagcggcggc agcgcagcac gccattgagt
     1921 ggcaacggca attcgcgctg caacagatcg cattcgtggc aggcaatcag gcgcccggtc
     1981 gtcatcgtgg caactccgca tgcgcctcgc ccgtcttttg gacgacactg ccctcctgct
     2041 gcggagtcgt gtgcgtcatc tgctccgcgg tgaccaccca gccgccgccc atcgccttgt
     2101 aaagcgcaac gagcgagctg aacaccgcgc cgttggtctg cgtatagctg agctgcgcgt
     2161 taaacaggct gcgttcggcg tccagtactt cgatatagct ggtgtagccg ccttcaaaac
     2221 gcagccgtgc aagccgcgca taagtgcgta gcgcatcgac ctggcgctct tgaacgacga
     2281 gttgctcgcg cgagttcttc accgagatga gcgcatcgtc gacctcctga aacgcggtct
     2341 gaatcgcctg ctggtactgg aacaaggcct gttgttgctg cgcttcggcc tgccgcacct
     2401 gaccgctgat gctgccggcg gtgaagatcg gcacgctcac cgaacctgca aacgaccaga
     2461 cccgcgccgg tccggtaaac agcttcgaga attcgacgct ggccgagcca aacagtccag
     2521 tcagtgaaat cgacggaaaa tagagtgcgc gagcggcgcc gatcagcgcg ttggcagcca
     2581 ccaggttctg ctcggcctgc agcagatccg gacgccgtgt gagcagatcc gagggcaacc
     2641 cggcgggcac cgccggaaca gccagatcgg acagttcgcg gccgcgcacg atgtcgcccg
     2701 ggttacggcc cagcaggatc gacagcgcgt tttcctgctg ggcgatctgc gtctggagct
     2761 ggggaatcgt cgcgagcgat gcctcgtatt ccgactggct ctgcgccagc tccatttgcg
     2821 atacgtcgcc ccctttgaac cgcaacgtaa agacgtgcac cgattccgcg cgggactctg
     2881 tcgtggactt cgcgatctcg agctgctggt cgagatcgcg cagattgatg taggccgtcg
     2941 ccaccgacgc gaccagcgtc aggatcgtcg cgcgccgccc ttcctcgctg gccagcagcc
     3001 cggcgcgcgc cgcttcggtc tggcggcgta ccctgccgaa cacatcgatt tcccaggccg
     3061 ccgacaacgc cggctcgaag atgttgaaga ccggcgacaa ccccgccggc agcggcacgg
     3121 cgccggccgt cgagacgccc tggcgttgcg catcgaaccc ggcgctcatc tgcgggaaca
     3181 gccccgcgcg ggtggtgacg aactggccga gaaactggtc gacgcgtgcg gccgcgatct
     3241 tcacatcctt attttctgcc agcgcggtgg cgatcagatc gttcagcacc gggtcctgaa
     3301 actgctccca ccacgcggta ttggccacct cgacggtctc accggccgcg aagcgatacg
     3361 tggccggtgt gtcgatcgcc ggccgcgagt agttcgggcc gagcagacag ccgcccagac
     3421 tcagcagcac gggcaccaga aggggcaagg acgctctgct acgcatgtca ttcgccctcg
     3481 tgtctggccg acggcgtgat cgtaggagcg ccgccggcgc ctgtgccgcc ctcggcgtcg
     3541 tcgccaggct tgctggcggt cggccccggt ttcttcttgc cgccgctttt ctctgacatc
     3601 gactcgagca ggtagaagaa catcgggatg aagaacacgg caatcgccgt cgcgccgagc
     3661 atgccgccga tcacgcccgt gccgatcgaa tggcggctgt tcgcggacgc accgagtgcg
     3721 atcgccagcg gcacgcagcc gaggatgaac gcgagcgacg tcatcacgat cgggcgcaga
     3781 cgttcggtcg ccgccgagac tgctgagtcg tacaccgact cgcccgactc gcggttgatc
     3841 accgcaaact cgaagatcag gatcgcgttc ttggccgcga gcgcgaccag catcgtcaga
     3901 ccgatctgga agtagacgtc gttttcgagg ccgcgcagca tgatcgcgag cagcgcgccg
     3961 aacagcgcga acggcacggc catcagtacc ccgaacggca ggctccactt ctcgtattgc
     4021 gcggccagaa tcaggaacac catgatcagg ccgaacacga acaccagcgc ggaggtcccg
     4081 ccggctttcc tggcctcgta cgcctcgccg ctccagccga ggctatagcc gtccggcagc
     4141 acctgcgcac ccacttcgtc gagcgtgttg agcacctggc ccgaactgat acccggagcg
     4201 ggattgacgg tgaccttcac ggccgggaaa ttgttgaacc gcgtcaccag atcaggtccc
     4261 gtgtcgaact tcgaggtcac gagcgtcttg agcggcacca tgctgttggt cttgctgcgc
     4321 acgaagatct gatcgagatc ctgaggcttg aggcggtagg acggttcggc ctgcaggatc
     4381 acctgccaca ggcggctcga acgattgaac tgcgagacgt acagcgaacc gaacatcgtc
     4441 tgcatcgcgc tgtagacgtc ctcgatcggc acacccagcg tctccgactt gtcgcggtcg
     4501 acgttcacca gcagttgctg cgcggatgca ttgaaggtcg acgtgacgcg cgccagttcg
     4561 ggccgggcct tggccttggc gacaaactgg tctaccacgc ccgccagttg cgcggtggtg
     4621 gcatcgccct gcgcctggac ccatacctcg gtgccgccgg tcgtgcccag gccgggaatc
     4681 gacggcggat tgaccggcag gatgatcccc tcctgcaccg ccgagagatt ctcgtacgcg
     4741 tcgatcagca ccgcgcgcgc gttctgcgtc ttgatgttgg cgaacttgta tcgttcgtcg
     4801 aaactcttga agccgataaa aaacgttccg gcattgttct tgttctgact gtcgagaagg
     4861 ctgaagccgt tgacgatcgt cacgctgccc acggccggct gcttcatgaa atagtccgtg
     4921 acatgctgcg tgaccgcctc cgtgcgatcg aggctcgcgg cgtccggcat gatgaccgcg
     4981 ccgagcaggt aaccctggtc ctccggcggc aggaacgagg tgggaatcga gcgcatcatg
     5041 acgaccgaga gcacaatcat gcccacaaaa atgagcagcc caatcacgga acgcttgatg
     5101 gcgagcttga ccaggttcgc atagccggtc gtcatccgcg cgaacgcgtt gtcgaaccac
     5161 ctgaagaaac cgcgcttttc atggtgaccg ggcttcagga gcaacgccgc cagcgccggc
     5221 gacaaggtca gcgccaccac cccggacagc accaccgaga tcgcgatggt gata
//
LOCUS       DATASH010000530         5606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3052385,
            whole genome shotgun sequence.
ACCESSION   DATASH010000530 DATASH010000000
VERSION     DATASH010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5606
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3052385"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="VHV99_14645"
     CDS             <1..742
                     /locus_tag="VHV99_14645"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HEX3636695.1"
                     /translation="KIHALTMSHELTGKSRLAGYYIDPSLRGDAAAHLMSRARMMYIA
                     ANRKRFTSEVFSLLLGVTDDAGVSPFWEAVGRKFFGRDFADIEIESGGRSRTFIAEVM
                     PTYPIYVPLLPEAAQRVLGEPDSKALLAYEIHLEEGFETDRFIDIFDAGPVLTAQVDR
                     SACVTRNETRVVREASAAAGGSTYLIAHNGAEGEFRCVLGELPDGKTSGASLSHAARA
                     ALGVQEGDAVRCVPLHQPHQEALTGDAP"
     gene            739..1776
                     /gene="astA"
                     /locus_tag="VHV99_14650"
     CDS             739..1776
                     /gene="astA"
                     /locus_tag="VHV99_14650"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HEX3636696.1"
                     /translation="MIVVRVVQRGDVDALMRLAQETGPGLTTFKPDREALAARVERAR
                     RTMEDKAEPHEAGYFFVMEDTETGDVAGVCGIETAVGLQQPFYNYRVSTVVHASQDLG
                     IWTRMRALNISHDLTGYAEVCSLFLSPRYRTSGVGGLLSRSRFMFLAQFRERFPQRLC
                     AELRGHFDAQGTSPFWRAVGSHFYQIDFNAADYLSSHGRKAFLAELMPRYPVYVELLP
                     EEAQECVGLTHSDTIPARRMLESEGLRYENHVDIFDAGPVLECHIADLRTVRESVVVP
                     VEIADVPAGAPQDAPRSLVSNTSLGDFRVGAAAGVPQDGVFRMSAAEAAALDVKAGDP
                     VRVLPLKHKQG"
     gene            1779..3242
                     /gene="astD"
                     /locus_tag="VHV99_14655"
     CDS             1779..3242
                     /gene="astD"
                     /locus_tag="VHV99_14655"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011656401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HEX3636697.1"
                     /translation="MSELFIDGEWAAGTGPAFASRNPGTGATVWQGNSASADDVDRAV
                     RCARRAFGRWSALSLDERCSVVRRFAALVTERKEALAEAIGRETGKPLWEARTEAASM
                     AAKVEISIQACNERTGEKRSPIADGTAVLRHRPHGVVAVFGPYNFPGHLPNGHIVPAL
                     IAGNAVVFKPSELAPGVAALTVQIWRDAGLPAGVLNLVQGEKETGIALANHRQIDGLF
                     FTGSSDTGTLLHKQFGGRPEIVLALEMGGNNPLVIGPVADVDAAVHHTIQSAFLSAWQ
                     RCTCARRIFVPNDAFGDRFVKRLTEVTSRISVGEYNAEPQPFMGAVISARAASRLVAA
                     QERLLADGAKALLKMEQRDPQLGFVTPAILDVTAVKDLPDEEHFGPLAQIVRYGTFEE
                     ALDQANDTEFGLSAGLLADDEALWVHFQRTIRAGIVNWNRPTNGASSGAPFGGPGRSG
                     NHRPSAYYAADYCAYPMASVESAQLQMPASVSPGLQF"
     gene            3252..4592
                     /gene="astB"
                     /locus_tag="VHV99_14660"
     CDS             3252..4592
                     /gene="astB"
                     /locus_tag="VHV99_14660"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006025855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HEX3636698.1"
                     /translation="MQATEANFDGLVGPTHNYAGLSFGNVASQSNDKSIANPKAAAKQ
                     GLRKMKQLAELGFHQGVLPPQERPSMRLLRELGFSGDDATVIARVAKDAPELLAAASS
                     ASAMWTANAATVSPSADTHDGRVHFTPANLCSKLHRAIEHESTRRTLRAIFSDADRFA
                     VHEALPGTPALGDEGAANHTRFCSEYGTRGVEFFVYGRSEYRRGPEPKRFPARQTFEA
                     SRAVAHRHGLAEAATVYAQQNPDVIDAGVFHNDVIAVGNRNTLFCHQLAFVEQKSVYD
                     ELRTKLAGLKAEFNVIEVPDAQVSVSDAVTSYLFNSQLLTRPDGKQVLVVPQECRENA
                     RVGAYLDDLTSHTGPIDDVLVFDLRESMKNGGGPACLRLRVVLNDAERAAVTPGVWIN
                     DKLFGRLDTWIEKHYRDRLAPADLTDPQLLAESRTALDELTQILGLGSLYDFQR"
     gene            4600..>5606
                     /gene="astE"
                     /locus_tag="VHV99_14665"
     CDS             4600..>5606
                     /gene="astE"
                     /locus_tag="VHV99_14665"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HEX3636699.1"
                     /translation="MSIPMLDDFLAYTLAGMRPGVLEAQGACANHSVRWSWLDDGVLL
                     MEPATQAESVRSVLASAGVHGDETAPIELLSRLVRDIADGGAALTCRVLVILGNVDAM
                     RDACRYRDDDLNRLFSGRHAQLPHSHEAPRAAALERMATQFFAAASQEPGARWHVDMH
                     TAIRASAFEQFALLPHTGKPFSRAMFEWLGEARISAVLLHTTKGNTYSHFTAQACGAE
                     ACTLELGKVRPFGQNNLTRFAGADEALRHLLAGTKNGNVPTPTPRVFTVIDQLTKQSE
                     AFELLVAADVPNFTPFVKDTVLARDGDYRYTVRHDEERIVFPNATVKPGLRAGLLVVE
                     TT"
BASE COUNT          972 a         1720 c         1882 g         1032 t
ORIGIN      
        1 caagattcac gcgctgacca tgtcgcatga attgaccggc aagagccgtc tggcgggtta
       61 ctacatcgat ccgtcgctgc gcggcgatgc ggccgcgcat ctgatgtcgc gggcgcggat
      121 gatgtacatc gccgcgaatc gcaagcgttt cacgtctgaa gtgttttcgc tgctgctcgg
      181 cgtcacggac gacgcaggcg tgtcgccgtt ctgggaggcg gtgggccgca agttcttcgg
      241 ccgcgacttc gccgacatcg aaatcgaatc gggtggccgc agccgcacct tcatcgccga
      301 agtgatgccg acctatccga tctacgtgcc attgctgccg gaagcggcgc agcgcgtgct
      361 cggcgagccg gattcgaagg cgctgctcgc gtatgagatt catcttgaag aaggcttcga
      421 gacggatcgc ttcatcgata ttttcgacgc gggtccggtg ctgaccgcgc aggtggatcg
      481 cagcgcctgc gtgacgcgca atgaaacgcg tgtggtgcgg gaggccagcg cggctgccgg
      541 cggctcgacg tatctgatcg cgcataacgg tgcggagggt gagttccgct gcgtgctcgg
      601 tgaattgccg gacggcaaga caagcggtgc gtcgttgtcg catgcggcgc gtgccgcact
      661 cggcgtgcag gagggcgacg cggttcgctg cgtgccgctg catcaaccgc atcaggaagc
      721 attgacggga gacgcaccat gatcgtcgtt cgcgttgtgc aacgaggcga tgtggacgcg
      781 ctcatgcggc tggcgcaaga gaccgggccc ggcctcacga ccttcaagcc ggatcgcgag
      841 gcactggcgg cgcgggttga acgtgcgcgc cgcacgatgg aagacaaggc tgaaccgcac
      901 gaagcgggtt acttcttcgt catggaagac accgagaccg gcgacgtggc cggcgtatgc
      961 ggcatcgaaa ccgcagtcgg tctgcagcaa ccgttctaca actaccgcgt gagcacggtc
     1021 gtgcacgcga gccaggatct cggcatctgg acacgcatgc gcgcgctgaa tatttcgcat
     1081 gacctcacgg gttacgccga agtgtgttcg ctgtttctga gcccgcgtta ccgcacgagc
     1141 ggtgtaggcg gcttgctgtc gcggtcgcgc ttcatgtttc ttgcgcagtt ccgcgagcgt
     1201 tttccgcagc gtctgtgcgc ggaattgcgt ggccatttcg atgcgcaagg cacctcgccg
     1261 ttctggcgtg cggtcggctc gcatttctac cagatcgatt tcaacgcggc ggactatctg
     1321 agctcgcacg gccgtaaggc gtttcttgct gagctgatgc cgcgttatcc ggtgtatgtc
     1381 gaactgctgc cggaagaagc gcaggaatgc gtcggcctca cgcatagcga cacgattccg
     1441 gcgcgccgca tgctcgaatc ggaagggctg cgctacgaga atcacgtcga tattttcgac
     1501 gcaggcccgg tgctcgaatg ccatatcgcc gacttgcgca cggtgcgcga gagcgtggtg
     1561 gtgccggtgg aaatcgccga cgtgccggcg ggcgcaccgc aggatgcgcc gcgttcgctg
     1621 gtatcgaata cgtcgttggg cgattttcgc gtgggcgcgg ccgcgggcgt gccgcaagac
     1681 ggcgtgttcc gcatgagcgc cgccgaggcg gccgcactcg acgtcaaggc aggcgacccg
     1741 gttcgggtgc tgccgctgaa acacaaacaa ggatgatcat gagcgagctt ttcatcgacg
     1801 gcgaatgggc cgccggcaca ggacccgcat tcgcgtcgcg taacccgggt acgggcgcga
     1861 cggtttggca aggcaacagc gcgtcggcgg acgacgtcga tcgtgcggtg cgctgcgcgc
     1921 ggcgtgcgtt tggccggtgg tcggcgttga gcctcgacga gcgttgcagc gtggtgcgcc
     1981 gcttcgctgc gctcgtgacc gaacgcaagg aagcgcttgc cgaagcgatc ggccgcgaga
     2041 ccggcaagcc gctgtgggaa gcgcgcacgg aagcggcttc gatggcggcg aaggtcgaga
     2101 tttcgattca ggcctgtaac gagcgcaccg gcgaaaagcg ttcgccgatt gccgatggca
     2161 cggccgtgtt gcggcatcgc ccgcacggcg tggtcgcggt gttcgggccg tataacttcc
     2221 cgggccattt gccgaacggg catatcgtgc cggcgctgat cgcgggtaac gcggtggtgt
     2281 tcaagccgtc cgaactcgcg ccgggcgtgg cggcgctcac ggtgcagatc tggcgtgatg
     2341 cgggtttgcc ggcgggcgtg ctgaacctcg tacaaggcga gaaagagacg ggcattgcgc
     2401 tcgccaacca tcggcagatc gacggtctgt tctttacggg tagttcggat accggaacat
     2461 tactgcataa gcaattcggc ggccgcccgg agattgtcct cgcgttggag atgggcggca
     2521 ataatccgct cgtgatcggg ccggtggcgg atgtcgacgc cgccgtgcat cacacgattc
     2581 agtcggcgtt tctgtcggcc tggcagcgtt gtacgtgcgc gcgccgtatt ttcgtaccga
     2641 acgacgcgtt cggcgaccgc tttgtgaaac gcctgaccga agtgacttcg cgcatcagcg
     2701 tcggcgaata caacgccgag ccgcagccgt tcatgggcgc ggtgatctcc gcgcgtgcag
     2761 cttcgcgcct cgtcgcggcg caggagcgtc tgctcgccga tggcgcgaaa gcgctgctga
     2821 agatggagca gcgcgatccg caactcggct tcgtcacgcc cgcgattctc gacgtgaccg
     2881 ccgtgaagga cctgccggac gaagaacatt tcggaccgct ggcgcagatc gtccgctacg
     2941 gcaccttcga agaagcgctc gaccaggcca acgacaccga gttcggcctt tccgccggcc
     3001 tgctcgccga cgacgaagcg ctgtgggtgc atttccagcg taccatccgc gcaggcatcg
     3061 tcaactggaa ccggccgacc aatggagcgt cgtcgggcgc accgttcggt ggcccggggc
     3121 gttcgggcaa tcaccggccg agcgcgtact acgccgccga ctactgtgcg tatccgatgg
     3181 cttccgtcga aagcgcgcaa ctgcaaatgc cggcgagcgt ttcgccgggt cttcaattct
     3241 aaggatcgac gatgcaagcc actgaagcca atttcgacgg tctcgtcggt ccgacccaca
     3301 attacgcggg gctctcgttc ggcaacgtcg cgtcgcagag caacgacaag tcgattgcca
     3361 accctaaagc ggccgcgaag caaggcctgc gcaagatgaa gcagttggcc gagctcggct
     3421 ttcatcaggg tgtattgccg ccgcaggaac gtccttcgat gcgcctgttg cgcgaactgg
     3481 gcttttccgg cgacgacgcc acggtgatcg cacgcgttgc gaaagacgcg cccgagttgc
     3541 tggccgcggc gagttcggcc tcggcgatgt ggaccgcgaa tgcggcgacg gtgagtccgt
     3601 cggccgatac gcacgacggc cgcgtgcatt tcacgccggc caatctgtgc agcaagctgc
     3661 atcgcgcgat cgagcatgaa tcgacgcgcc gcacgttgcg cgcgatcttt agcgatgccg
     3721 atcgttttgc tgtgcacgag gccttgcccg gcacgcccgc tctcggcgac gaaggcgcgg
     3781 cgaaccacac gcgtttttgc tcggaatacg gcacacgtgg cgtcgaattc ttcgtgtacg
     3841 gccgcagtga atatcgccgc ggaccggagc ccaagcgttt ccccgcgcgc cagacgttcg
     3901 aggcgagccg cgcggtcgcg catcgtcacg gtctggccga agcggccacg gtgtacgcgc
     3961 agcagaatcc ggacgtgatc gacgcgggcg tgttccataa cgacgtcatc gccgtcggca
     4021 atcgcaacac gctgttctgc catcaactcg cgtttgtcga acagaaatcg gtgtacgacg
     4081 aattgcgcac gaagctcgcc gggctgaaag ccgagttcaa tgtgatcgag gtgcccgacg
     4141 cgcaagtgag cgtgtccgac gcggtcacgt cgtacctgtt caacagccag ttgctgacac
     4201 gcccggacgg caagcaggtg ctggtggtgc cgcaggagtg ccgcgagaac gcgcgcgtgg
     4261 gcgcgtatct ggacgatctg acttcgcaca ccggcccgat cgacgatgtg ctcgttttcg
     4321 atctgcgcga aagcatgaag aacggcggcg gtccggcatg tttgcggctg cgcgtggtgt
     4381 tgaacgatgc ggagcgcgcg gcggtgacgc ctggcgtgtg gatcaatgac aaactgttcg
     4441 gccgcctcga tacgtggatc gaaaagcatt accgcgatcg tcttgcgccg gccgatctga
     4501 ccgacccgca attgctcgcc gagtcgcgca ccgcgctcga tgaactgacg cagattctcg
     4561 gtctgggttc gctgtatgac ttccagcgct gagcgcggca tgtcgattcc gatgctcgac
     4621 gatttcctcg cctacacctt ggcgggcatg cggcctggcg tgctcgaagc gcaaggtgcg
     4681 tgcgccaacc atagcgtgcg ctggtcatgg ctcgacgacg gcgtgctgct catggagccg
     4741 gccacgcagg cggagagtgt gcgcagtgtg ctcgcctctg ccggcgtgca tggtgatgaa
     4801 accgcgccga tcgaactgct gtcgcgtctc gtgcgcgata tcgcagatgg cggcgcggcg
     4861 ttgacatgcc gcgtgctggt gattctcggc aacgtcgatg caatgcgcga cgcgtgccgt
     4921 tatcgcgacg acgatctgaa ccgccttttc agcgggcgtc acgcgcagct gccgcacagc
     4981 cacgaagcgc cgcgcgcagc ggcattggag cggatggcaa cgcagttctt tgcggctgcc
     5041 tcacaagagc cgggcgcccg ttggcacgtg gacatgcaca cggcgatccg cgcttcggct
     5101 ttcgagcagt tcgccttgtt gccgcatacc ggcaagccgt tttcgcgcgc aatgttcgag
     5161 tggctcggcg aagcgcgcat tagtgcggtt ctattgcaca ccaccaaggg caacacgtac
     5221 tcgcacttca ccgcgcaagc gtgcggcgcc gaagcatgta ccctcgaact cggcaaggtg
     5281 cgccccttcg ggcagaacaa tctgacgcgt ttcgccggcg cggacgaggc gttgcgccat
     5341 ctgctggccg gtacaaagaa cggcaacgtg cccacgccga cgccgcgcgt attcaccgtg
     5401 atcgaccagc tcaccaagca gagcgaagca ttcgaattgc tggttgccgc ggacgtgccg
     5461 aatttcacgc cctttgtaaa ggacaccgtg ctcgcacgcg acggcgacta tcgctacacc
     5521 gtgcgtcacg acgaagaacg tatcgtgttt cccaatgcga cggtcaagcc gggtttgcgc
     5581 gcgggtctgc tggtcgtcga gaccac
//
LOCUS       DATASH010000531         3586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5625911,
            whole genome shotgun sequence.
ACCESSION   DATASH010000531 DATASH010000000
VERSION     DATASH010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5625911"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="VHV99_14670"
     CDS             <1..391
                     /locus_tag="VHV99_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX3636700.1"
                     /translation="AGKITQATRRDPRITAVGAFLRRTSLDELPQFINVLRGEMSVVG
                     PRPHALEHDDIYKDLVKGYMHRYRIKPGITGWAQINGYRGETDRIEKMMGRVKLDLYY
                     MQHWTFWLDIKIVVLTLWKGFVGSNAY"
     gene            567..2030
                     /locus_tag="VHV99_14675"
     CDS             567..2030
                     /locus_tag="VHV99_14675"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEX3636701.1"
                     /translation="MNLTIIGSGYVGLVTGACLADIGHDVFCLDVDQRKIDVLNNGGV
                     PIHEPGLQEIIARNRKAGRLTFSTDIEAAVAHGDIQFIAVGTPSDEDGSADLQYVLAA
                     ARNIGRHMTGFKVIVDKSTVPVGTASRVRDVIGGELAARKASHMFSVVSNPEFLKEGA
                     AVEDFTRPDRIVLGCDEDAPGEKARELMKRLYAPFNRNRERTLYMDVRSAEFTKYAAN
                     AMLATRISYMNELANLADRVGADIEAVRRGIGSDPRIGYDFLYAGCGYGGSCFPKDVQ
                     ALIRTAADHKANLRILEAVEAVNDTQKKILAHKIVARLGEDLSDRTFGVWGLAFKPNT
                     DDMREAPSRPLIAELLRRGARVKAYDPVAIDESKRVFALDLKDVPQQHARLSFVNEEM
                     DAAVGADALVILTEWKVFKSPDFGSLKQSLKTPLIFDGRNLYEPDALLELGIEYHAIG
                     RQHALRTAPGKVAANGLPITAAAAGMAQTVDSAEAVR"
     gene            2059..2514
                     /locus_tag="VHV99_14680"
     CDS             2059..2514
                     /locus_tag="VHV99_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018431976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEX3636702.1"
                     /translation="MFANVLIVCHANVCRSPAAEMLFKARQHTSSRPSSAPIAFHSAG
                     IRAVNGHAMDPVMRRLLAEQGVASGIHYSRRLDRQLVRCADLVLVTERAQVSTVEALD
                     PASRGKVYPLGKWEDDSDVADPHGGAEADYRESLVLIEHLVMGWLKKIC"
     gene            2508..>3586
                     /locus_tag="VHV99_14685"
     CDS             2508..>3586
                     /locus_tag="VHV99_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEX3636703.1"
                     /translation="MLMKKLTAHTFANFNSTAFKSRFAATLLLTSFLSACATAPGNYL
                     DTSRLKDDGQHQSQQANETYPVHLIDGPLVAAQAQAQANAAQQALPTSRYSDPSQYVY
                     RLSPQDILGITVWDHPELTTPQGSTLSAGGNTTQSIGGALQQPYTAALPGQADPYGQT
                     IAADGTIFFPFVGRIQAAGKTVGELRDQLSAGLVRYIRNPQVDVRVLSYRGQKVQVTG
                     DVKTPGPLAISDVPLTLVDAITRSGGTNSDADIQRVRLTRNNKLYVLDANRMLDQGDT
                     TQNVMLQNGDVINVPDHSDSRVFVMGEVKTPIQVPMIRGRLTIADALTLGGGILDTDA
                     NPRQIFVMRGMREHPTTPDVYRLDMT"
BASE COUNT          689 a         1196 c         1106 g          595 t
ORIGIN      
        1 ggccggcaag atcacccagg ccacgcggcg cgatccgcgc atcaccgcgg tcggcgcgtt
       61 cctgcggcgc accagcctcg acgaattgcc gcaattcatc aacgtgctgc gcggcgagat
      121 gtcggtggtc ggcccgcgtc cccacgcgct cgaacacgac gacatctaca aggatctggt
      181 gaagggctac atgcaccgtt accggatcaa accaggcatc accgggtggg cgcagatcaa
      241 cggttatcgc ggcgaaaccg atcgcatcga aaagatgatg ggccgcgtca agctcgatct
      301 gtactacatg cagcattgga ccttctggct cgacatcaag atcgtcgtgc tgacgttgtg
      361 gaagggcttc gtgggcagca atgcatattg agagcccccc aactggcgct tcgcgccagc
      421 ccccccgagg gggggcagga aaacttgggg cggcccggcg ttttcctgcg agagccccca
      481 cctggcgctt cgcgccagcg cttaagcatc gcaacgcatc gcgtgactta tttagtgaat
      541 caactccatt ttccgaggtg taatagatga acctgacgat catcggtagt ggctacgtag
      601 gccttgtgac gggcgcatgt ctggccgaca tcggccatga cgtgttctgt ctcgacgtcg
      661 atcaacgcaa gatcgacgtg ctcaacaacg gcggcgtgcc gatccatgaa ccgggtctgc
      721 aggaaatcat cgcgcgcaat cgcaaggccg gccgcctgac gttctcgacc gacatcgagg
      781 ccgcggtcgc gcacggcgac atccagttca tcgcggtggg cacgccgtcc gacgaagacg
      841 gttccgccga tctgcaatac gtgctggccg ccgcgcgcaa catcggccgc cacatgactg
      901 gcttcaaggt gatcgtcgac aaatcgacgg tgccggtcgg cacggcttcg cgcgtgcgcg
      961 acgtgatcgg cggagaactg gccgcgcgca aggcgagcca catgttctcc gtggtgtcga
     1021 atccggagtt cctgaaagag ggcgcggccg tcgaagactt cacgcgcccg gaccgcatcg
     1081 tgctcggctg cgatgaagac gcgcccggcg aaaaagcgcg cgaactgatg aagcgtctct
     1141 atgcgccgtt caaccgcaat cgcgaacgca cgctctacat ggacgtgcgt tcggccgaat
     1201 tcaccaagta cgcggccaat gccatgctcg ccacgcggat ctcgtacatg aacgagctcg
     1261 cgaatctcgc cgaccgcgtc ggcgcggata tcgaagcggt gcgccgcggc atcggctccg
     1321 atccgcggat cggctacgac ttcctgtatg cgggttgcgg ctacggcggc tcgtgctttc
     1381 cgaaagacgt ccaggcgctg attcgcacgg cggcggatca caaggccaat ctgcgcattc
     1441 tcgaggcagt ggaagccgtc aacgacacgc agaagaagat ccttgcgcac aagatcgtcg
     1501 cgcgtctggg cgaagacctg tcggatcgca cctttggcgt gtggggcctt gccttcaagc
     1561 cgaacacgga cgacatgcgc gaagcgccga gccgtccgct gatcgccgaa ctgctgcgcc
     1621 gcggcgcgcg cgtgaaggcc tacgacccgg tggcgatcga cgaatcgaag cgcgtcttcg
     1681 cactcgatct gaaggacgtg ccgcaacagc acgcgcgttt gtcgttcgtg aacgaggaga
     1741 tggacgcggc cgtgggcgcc gatgcactgg tgattctcac cgagtggaaa gtcttcaaga
     1801 gcccggattt cggttcactc aaacagagtc tcaagacgcc gctgattttc gacggccgca
     1861 atctgtacga gccggacgcg ctgctcgaac tcggtatcga gtatcacgcc atcggccgtc
     1921 agcacgcgct gcgcaccgcg ccggggaaag tcgccgcaaa cggcctgccc atcacggccg
     1981 ccgcggccgg catggcccag acggtcgatt cggccgaggc cgtccggtaa gccgccacgc
     2041 ctgatcaagg agcccgccat gttcgccaac gttctgatcg tctgccacgc caacgtgtgc
     2101 cgttcgcccg ccgccgaaat gctgttcaag gcccggcagc acacgtcgtc gcggccgtct
     2161 tcagcgccga tcgcgtttca ttcggcgggc atccgtgccg tgaacggcca cgccatggac
     2221 ccggtgatgc ggcgcctgct cgccgagcag ggcgtcgcct ccggcatcca ttactcacgg
     2281 cgcctcgacc gccaactcgt gcgttgcgcc gacctcgtgc tcgtcaccga gcgcgcacag
     2341 gtgagcacgg tcgaagcgct cgatccggcc tcgcgcggca aagtctatcc gctcggcaag
     2401 tgggaagacg attccgacgt cgccgatccg cacggcggcg cggaagccga ctatagggag
     2461 agtctcgtgc tcatcgaaca cctggtcatg ggatggctga aaaaaatatg ctgatgaaga
     2521 aactcactgc acacacgttc gcgaacttca attcgacggc ctttaagtcc agattcgccg
     2581 ccacgctttt gctgacgtcc tttttgtcgg cctgcgctac cgcacctggc aattacctcg
     2641 acacgtcccg tctgaaagac gacggccagc accagagtca acaagccaac gaaacctatc
     2701 cggtccatct gatcgacggg ccgctcgtcg cggcccaggc ccaggcgcaa gccaacgccg
     2761 cgcagcaggc gctgccgacc tcgcgttaca gcgatccgtc gcagtatgtg taccggctct
     2821 cgccgcagga cattctcggc atcaccgtgt gggaccaccc tgaattgacc acgccgcagg
     2881 gcagcacgct gtcggcgggc ggcaacacca cgcagtcgat cggcggcgct ttgcagcagc
     2941 cgtataccgc cgcgctgccg ggccaggccg atccttacgg ccaaaccatc gccgccgacg
     3001 gcacgatctt tttcccgttt gtcggccgca tccaggccgc gggcaagact gtcggcgagt
     3061 tgcgcgatca attgagcgcc ggcctcgtgc gctacatccg caatccgcag gtcgacgtgc
     3121 gcgtgctgtc gtatcgcggc cagaaagtgc aggtgaccgg cgacgtgaag acgccgggcc
     3181 cgctcgccat cagcgacgtg ccgctcacgc tggtcgacgc catcacgcgc tcgggcggca
     3241 ccaacagcga cgccgacatc cagcgtgtgc gcctcacgcg caacaacaag ctgtacgtgc
     3301 tggatgccaa ccgcatgctc gaccagggcg acaccacgca gaacgtcatg ctgcaaaacg
     3361 gcgacgtgat caacgtgccg gaccacagcg acagccgcgt cttcgtgatg ggcgaagtga
     3421 agacgccgat ccaggtgccg atgatcaggg gccgcctgac gatcgccgac gcgctcaccc
     3481 tggggggcgg cattctcgat acggacgcca atccgcgcca gatcttcgtg atgcgcggca
     3541 tgcgcgagca tccgaccacg ccggacgtgt accgcctcga catgac
//
LOCUS       DATASH010000532         3834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_878139,
            whole genome shotgun sequence.
ACCESSION   DATASH010000532 DATASH010000000
VERSION     DATASH010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_878139"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..1160
                     /locus_tag="VHV99_14690"
     CDS             168..1160
                     /locus_tag="VHV99_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEX3636704.1"
                     /translation="MNSNAVIAIHGGAGTILRTSMSASAEADYHAALNAVLSAGQRVL
                     ADGGSALDAVSEAVRLLEDCALFNAGHGAVYTAAGTHELDAAIMDGSTLEAGAICCVK
                     RVRNPILAARRVLERSEHVLFTGEGAEAFAAAQGLEFAEPAYFHTEARHRQWLIARGQ
                     QRAMLDHDGATLSAAPSPSDDDPVPHEPIDPNRKFGTVGAVALDQHGHVAAATSTGGV
                     TNKQVGRVGDTPLIGAGCYADDATCAVSTTGSGEMFMRMVAAYDVAAQMAYRNVSLEE
                     AAHDVVMNRLPKIDGRGGLIAVDARGNITLPFNTEGMYRGFARIGETPVTAIYR"
     gene            1260..1577
                     /locus_tag="VHV99_14695"
                     /pseudo
     CDS             1260..1577
                     /locus_tag="VHV99_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180648.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            1574..2308
                     /locus_tag="VHV99_14700"
                     /pseudo
     CDS             1574..2308
                     /locus_tag="VHV99_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589373.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
     gene            complement(2333..3319)
                     /locus_tag="VHV99_14705"
     CDS             complement(2333..3319)
                     /locus_tag="VHV99_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3636705.1"
                     /translation="MLPKRLLCAAALYLCFAGAGAFAQTAAPAAEPAAEPATEPAEMP
                     GMESSAPEAPAPPPAAPATPATPAKPAAATPAPAPPVTPRATAVTPPVAPVTGPVRNV
                     VLVHGAFLDGSSWNGVVAKLQQKGYHVSSVQNPLTSLADDVAATRRVLARQDGPTILV
                     GHSWGGVVITEAGANAPNVAGLVYVAAIAPDLHESTVDLMKRAAPAPATQGITADSMG
                     FLWLDRTKYHADFAADVPENLTRVLSAAQQPMAAKAFSETVSQVAWREKPSWYIVTTR
                     DRALSPEVEQFMANRMGAKIVPISSSHLVPVSHAGAVADVIDRAARELSRQQ"
     gene            3533..>3834
                     /locus_tag="VHV99_14710"
     CDS             3533..>3834
                     /locus_tag="VHV99_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017329535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HEX3636706.1"
                     /translation="MNSAQQQDGLKRGLKNRHIQLIALGGAIGTGLFLGSASVLQAAG
                     PSMILGYAIGGIIAFMIMRQLGEMVAQEPVAGSFSHFAYKYWGDFPGFLSGWNYWVL"
BASE COUNT          688 a         1218 c         1259 g          669 t
ORIGIN      
        1 cgcgccgcga gcaaaatcgc gcgcgcgaag gccgggaagg tcaaacacgg cgacggcgcg
       61 aaggccgaca acgccaaagc cgaacgcatg aaagccacgg accccgaagc aaaagccgtc
      121 gtggcagccc gcactccccg aaccaaccgc actcgtcaat ccaaacgatg aattccaacg
      181 cagtcattgc cattcacggc ggggcaggga cgatcctgcg cacgtcgatg tcggccagcg
      241 ccgaagccga ctatcacgcg gcgctcaacg cggtgctgag cgccggccag cgcgtgctcg
      301 ccgatggcgg cagcgcgctc gacgcggtca gcgaagcggt gcgtctgctc gaagactgcg
      361 cgctcttcaa cgcgggccac ggcgcggtct acaccgcggc cggcacgcac gaactcgacg
      421 cggcgatcat ggacggcagc acgctcgaag ccggcgcgat ctgttgcgtg aagcgcgtgc
      481 gcaatccgat tctggcggcg cgccgcgtgc tcgaacgcag cgagcatgtg ctgttcaccg
      541 gcgagggcgc ggaagcgttc gccgccgcgc aaggactcga attcgccgag ccggcgtatt
      601 tccataccga agcgcgccat cgtcaatggc tgatcgcgcg cggtcagcaa cgcgccatgc
      661 tcgatcacga tggagcgacg ctctccgccg cgccgtctcc gagtgacgac gacccggtgc
      721 cgcacgaacc gatcgatccg aaccgcaagt tcggcaccgt cggcgcggtc gcgctcgatc
      781 agcacggcca tgtggcggca gcgacctcga ccggcggcgt caccaacaag caggttggcc
      841 gggtcggcga tacgccgctg atcggcgcgg gctgctatgc ggacgacgcg acctgcgcgg
      901 tctcgaccac cggctcgggc gaaatgttca tgcgcatggt cgcggcctac gacgtcgcgg
      961 cgcaaatggc ctaccgcaac gtctcgcttg aagaagcggc gcatgacgtg gtgatgaacc
     1021 gtttgccgaa gatcgacgga cgcggcggtt tgatcgccgt cgatgcacgc ggcaacatca
     1081 cgctgccgtt caataccgag ggcatgtatc gcggtttcgc gcgaatcggc gagacgccgg
     1141 tgacggcgat ttatcgctag tgctgtttcg ctgcgctgcc gcctgaactg ctgtctgaat
     1201 cttcgcttga actttcgctt gaaccaccgt tagccgcgtt cgaaataaaa ggaaccatcg
     1261 tgccgacttc atcgcacacc gctcgtccgc ttatcgaaac cttgccgcca cactgcgtgc
     1321 ttgcggtcga cgatctgtcg gtcgcatttc gcagcggcga caaaaccttc aacgcggtgc
     1381 gcaatctctc gctgacggtc gagcgtggcg agacgctggc gatcgtcggc gaatcgggtt
     1441 ccggcaaatc gatgacgggc cgttcgttgc tgcgcctcgt ggaaagtcag agtggttcga
     1501 ttgaattcga cggcaaggaa atcagttcgc tcacaggccc tgcgttgcaa gcgctgcgcc
     1561 gcgatatcca gtttatatga cgctcgaagc gaacgccgcg atcgaaggcg cattcgccgg
     1621 cggcgcgacg catgtatggg tcaacgattc gcatggcggc tttcgcaatc tcctgcccga
     1681 tttgctcgac gcacgtgtcc aggtcgtgct cggcaatccg cgtacgctcg gcatgatggc
     1741 cgggcttgaa tacggtgcgg cgctggtgtt catgatcggc tatcacgcga agtcgcagac
     1801 gcgcggcatc ctcgcgcata cgatcaacag cttcgcgttc gcgcgcgttt cgctgaacgg
     1861 cgtcgaagtc ggcgaggcgg gattgtatgg agcgctagca gaagaatatg gtgcacaggt
     1921 agcgctttta tcgggcgacg atgtattcgc cgaagaaacc gcgccgcgct ttcccggcgc
     1981 gcgttttgtc gtcaccaaga gcgcgacggg gcatgcgagc ggtgttacgc aaaccccggc
     2041 gagcgcgcga gatacaatcg aaattgctgc acgcgaagtg gtgcggcact ccatcgacaa
     2101 cggtcatgcg ccacaagcgg cgcgcgctgc cgcgaagccc gtcgaatgcg aattgcgtgt
     2161 gcaaaccacc gcgctcgccg atctgttctg ccaatggccg acgctcacgc gcgtcgacgc
     2221 cgtcacgctg cgctttggcg cggagtcggc ggaacatgcg gtgcgtatgc tgaactgcct
     2281 gtcggcgatg tcgttcatgc tgcgctagcc gcgtggcgaa gtgcggcgcc ggctactgct
     2341 gccggctcaa ctcccgcgcc gcgcgatcga tcacatcggc gaccgccccc gcatgcgata
     2401 ccggcacgag atggctcgat gaaatgggca cgatcttcgc gcccatccgg ttggccatga
     2461 actgttcgac ttcgggcgac agcgcgcggt ccctcgtcgt cacgatgtac cacgacggct
     2521 tttctcgcca cgccacctgg cttaccgttt cgctgaacgc tttcgcggcc atcggctgtt
     2581 gcgcggcgga gagcacgcgc gtcagattct cgggcacgtc ggcggcgaag tcggcgtgat
     2641 acttggtgcg gtcgagccaa aggaagccca ttgaatccgc ggtgatgcct tgagtcgcgg
     2701 gcgctggcgc cgcgcgcttc atcagatcca ccgtcgattc atgcaggtcc ggcgcaatcg
     2761 cggccacgta gacaaggccc gctacgttcg gcgcattcgc gccggcttcg gtgatcacga
     2821 cgccgcccca cgagtgcccc accaggatgg tcgggccgtc ctggcgtgcg aggacgcggc
     2881 gggtcgcggc gacatcgtcg gcgagcgacg tgagcggatt ctgcaccgaa ctcacgtgat
     2941 agcccttttg ctgcaacttc gcgacgacac cgttccagct cgacccgtcg aggaacgcgc
     3001 catgaaccag cacgacgttg cgcacggggc cggtcaccgg tgctacggga ggtgtgacgg
     3061 cagtcgccct gggcgtgacg ggtggggcgg gtgcgggagt ggcggctgca ggcttggcag
     3121 gcgtggcagg cgtggcaggt gccgcaggcg gcggggccgg cgcttcaggt gcgctcgact
     3181 ccatgccggg catctcggcc ggttcggtgg cgggttcggc ggcgggttct gccgcgggcg
     3241 ccgcggtttg cgcgaaggcg cccgccccag cgaagcagag atacaaagca gcggcgcaaa
     3301 gcagacgttt gggcaacatg ttgaatctcc gaaggctgaa acctcgacta ggcgaccata
     3361 cagaagcgcc acgccagtga caaccactat cctcgccgtt aagtgcgacg cagaaccaag
     3421 cgcgggttgc tcccagtatt gaggtatttt cgggcacgct aaaatttcag gttctgcccg
     3481 gccggaccat aagtctgacc gggtgatcga tatccataca ctggggcgca atttgaatag
     3541 tgcacagcag caagacggcc tgaagcgcgg gcttaagaat cgccacatcc agctgattgc
     3601 gctgggcggc gcgatcggca ccggcttgtt tctcggctcc gccagcgtat tgcaggcagc
     3661 cggcccgtcg atgattctcg gctacgcgat cggcggcatc atcgctttca tgatcatgcg
     3721 gcagctgggt gaaatggtcg cgcaggaacc ggtcgccggt tcgttcagcc acttcgccta
     3781 caagtattgg ggcgactttc ccggcttcct gtccggctgg aactactggg tgct
//
LOCUS       DATASH010000533        17632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2854663,
            whole genome shotgun sequence.
ACCESSION   DATASH010000533 DATASH010000000
VERSION     DATASH010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 17632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17632
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2854663"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /locus_tag="VHV99_14715"
     CDS             complement(<1..898)
                     /locus_tag="VHV99_14715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HEX3636707.1"
                     /translation="MAVIRSDRPLDWAQVAAIAAGEPLELSADARSRIAAARALVVQI
                     VERGIRAYGVNTGVGALCDVIVSPAEQRTLSRNILMSHAVGVGAPLGIAETRAIMAAA
                     VNNFAHGHSGIRLEVADQLVALLNADCLPEVPAFGSVGYLSHMAHIALVCIGEGHVRY
                     RGERLKGRDALQLLGLEPLVLEAKEGLSLINGTPCVTGLAALALARAARLLDWTDVVA
                     AMSFENLRGQLAAFDEDSLALRISPGLNLVGERMRTALADSGILAAVVGQRTQDPLSM
                     RTIPHVHGAARDVLTATADVVNR"
     gene            complement(953..1744)
                     /locus_tag="VHV99_14720"
     CDS             complement(953..1744)
                     /locus_tag="VHV99_14720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3636708.1"
                     /translation="MNATAPVALSVKNIHKSFGDHHVLKGISLDAHQGDVISILGASG
                     SGKSTFLRCLNLLETPDDGSVALGGEELKMKRRGDGKLQPSDRRQVDRVRSQLGMVFQ
                     NFNLWSHMTVLENLIEGPMRVQKRSRAESVEEAEALLAKVGLAEKRGHYPAHLSGGQQ
                     QRVAIARALAMHPKVMLFDEPTSALDPELVGEVLRVMRSLAEEGRTMLVVTHEMGFAR
                     HVSNRVMFLHQGQVEADGTPDEVFVECKSDRFRQFVSSHQDRTTN"
     gene            complement(1779..2534)
                     /locus_tag="VHV99_14725"
     CDS             complement(1779..2534)
                     /locus_tag="VHV99_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013343105.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX3636709.1"
                     /translation="MHFDFDFLLDTLRQLIAAVPTTLGLFFCSLILGGLLSLVIVTMR
                     VSPHWLPNRFARAYILVFRGSPLLIQMFLVYYGMGQFGVIRESFLWPVLREPYMCAVL
                     SLALCTAGYTAEIIRGGLMAVPVGQIEAGYSIGLSGFALLRRVIGPIALRQCLPAYST
                     EAVLLVKSTALASLVTVWEVTGVAQQIIQQTYRTTEVFICAALIYLFLNFVIVRLLGF
                     LETRLSRHLRAAPASSSPNRPVSSTTEARRAAP"
     gene            complement(2550..3272)
                     /locus_tag="VHV99_14730"
     CDS             complement(2550..3272)
                     /locus_tag="VHV99_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007587161.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX3636710.1"
                     /translation="MALIEMLGFGPEGWGGVLLLAALMTIALTLAALAVGAVFGALIA
                     AAKLSRFRTLRVIGDVYTTVFRGVPELLVIYLFYFGGSTLVTTIGQWFGADGFVGVPP
                     FVIGALAVGMISGAYQAEVYRSAVLAVSRGELEAARSIGMPTMTMARRILIPQVLRFA
                     LPGIGNVWQLSLKDSALISVTGLAELLRASQVAAGSTHQYFTFFVVGGALYLLMTSIS
                     NRVFNRAEARVGRSFKRNFARN"
     gene            complement(3365..4189)
                     /locus_tag="VHV99_14735"
     CDS             complement(3365..4189)
                     /locus_tag="VHV99_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017773640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX3636711.1"
                     /translation="MGAVVGTATIFAAPLQAKDWKTVTIALEGGYAPWNLTLPGGKLG
                     GFEPELVANLCERIKLQCNLVAQDWDGMIPGLQAGKFDVLMDAISITPEREKIIAFSK
                     PYAATPATFAVTDAKVLPKAVPTAGVVKLSGDPKTDQPTVDALRKQLKGKTIGIQSGT
                     VYTKFINDGFKDVATIRVYKTSPERDLDLANGRIDASFDDVTYYAANIDKKETSSIVM
                     AGPKIGGPIWGPGEGLAFRKQDADLKAKFDTAISAALADGTVKKLSAKWFKTDVTP"
     gene            complement(4516..5196)
                     /locus_tag="VHV99_14740"
     CDS             complement(4516..5196)
                     /locus_tag="VHV99_14740"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX3636712.1"
                     /translation="MITRLILQTVVWLACMGALLFGAAGTFAWPAGWWYLIETGGLSL
                     WIGFWLARHDPGLLAERLAPIVQAQQSRWDRVFMVAVAVMWCAWLVLMGLDAMRYRWS
                     APLPVWLVSFGSLCIFVCLSMCLIVFRANSYAAPVVKIQASRGHKVIDTGPYAHVRHP
                     MYSAALLLFVGTPLLLGSWWGFACVPLLIVGIGWRAVREERVLAEQLDGYTDYTSRVR
                     YRFVPFIW"
     gene            complement(5276..6115)
                     /locus_tag="VHV99_14745"
     CDS             complement(5276..6115)
                     /locus_tag="VHV99_14745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HEX3636713.1"
                     /translation="MSHTVNLENYFTRIGYKGPRAATLEVLQALHQLHPATIPFENLN
                     PLTRRAVKLDHESVEQKLITQKRGGYCFEQNALFANVLMQLGFEVTPLLGRVLWGRES
                     AEIPPRTHMVLRVDINNEAWIADVGFGSVTLTAPLRLIAGAAQSTPLGTFRLADAAHD
                     ALYLEVQSPDETWGRVYRFDLHPVEWIDYETSNWYTSTAPDSVFLNNLIVCRVLAQSR
                     LTLLNDQLNERAADGHVISERQLKSADELATCLRDQFGLNTGDIDMAEVFGRVRSPAA
                     ASA"
     gene            complement(6321..7004)
                     /locus_tag="VHV99_14750"
     CDS             complement(6321..7004)
                     /locus_tag="VHV99_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636714.1"
                     /translation="MNKRILLLTLLASGGLLVSGVSSAQLNLKQLGIGGGNTSADAGA
                     TASSGGISQLLQTYVGANQQVLSGQSSLASAMGMSSAAGQAQQAAGLLSGGTPSVSQL
                     TQVGSTQQSLSQTLTQAFASRASGGASATPVNKQAFTDGLSSLGKGVSQYSSLQSGLG
                     SIGQMSPSSLLQSGMNPQTAQSASYIAQSAPGQLQSLMSTLSSAVQFASSHGISVPSI
                     ATSALKGGQ"
     gene            7432..8856
                     /locus_tag="VHV99_14755"
     CDS             7432..8856
                     /locus_tag="VHV99_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007743108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HEX3636715.1"
                     /translation="MRRAALCTAFAWASLNAGAAMAEANPDATPEAPEADLNIQATQT
                     NQWTGFWNRQTMLGDIGGLRPWLGKYGVTFALTETSEVLANLRGGLARGADYDGLTTA
                     ALQMDTQKAFGLPGGLFNVSALQIHGANLSANKLGTLNTASGIEADDATRLWELWYQQ
                     SFLNKRIDVKIGQQSIDQEFITSSNSALFVNTMFGWPALPSYDMPSGGPAYPLSGLGV
                     RVRGQITSSLTALAGVFDGDPLGNNPNNKSGTNFNLHNGTLFIGELQYAINQPADGEM
                     VGMSGGGLPGTYKLGVWYNNGSFADQRFDNTGLSLANPASTGVPQNHHGDYSFYAVAD
                     QMIWRPDPDEPRSLNVFARVMGAPGDRNLVSVAANLGIVMKAPFAGRDNDSAGIALTY
                     IKIGNHVNGLDQDNLAFSGGPYGVRTSETTLEATYQYQVNPWWQLQADAQYTFNAGAG
                     QNPNDPTQPLRNTFVIGVRTNITF"
     gene            8957..10108
                     /locus_tag="VHV99_14760"
     CDS             8957..10108
                     /locus_tag="VHV99_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636716.1"
                     /translation="MNNPTRKFSPRAARALIAATVSAAAFATAGVAHAEPQGFLETVK
                     HHTTLINTVPGNGDQNPYAVVVAPVSAGTVKQGDVLVGNFNNSTNLQGTGSTIINYHP
                     DTKQMTVFATVPRDLKECPGGIGLSTAMTMLKSGYVIVGSTPSNDGTTGTKGAGCLIV
                     IDPNGTVTSTITSPNINDPWGNMAVVDNGTSATLFVSNAGFGVGGAQGNPPVFKQATV
                     LRLDLDVPAGKPPVVKKETVVGSGFGAQADKGVFLVGPTGLALSNDQKLLYVSDAIGN
                     RITEIDDPMTRDTSAGVGRQLTADGLLHRPLAMVTAPDGHLLVTNALNGQIVEIDPAS
                     GKQIYARWIDTDKAQSPPGNGDLFGLAMTPEGDGFYYVQDDVNTLVLAK"
     gene            10270..11670
                     /locus_tag="VHV99_14765"
     CDS             10270..11670
                     /locus_tag="VHV99_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HEX3636717.1"
                     /translation="MANDQPPRPSRRGFLKAGGAAVAAGASLGAGLATSQTAQAALAR
                     TPAHHADPQTAVEPFYGLHQGGIVTPQQSHTYLAALDLTTDKRDDVIALLRTWTDTAA
                     RLTQGATAAPLPTGTAAKAELVDSKIDTQVDTKTDPKVDNTAYSPTNTKAAPDSGDVL
                     GLGPCGLTITFGFGPGLFTHEGKDRYGLASRRPAALVDLPRFNGDQLIAEKTGGDLFI
                     QACANDQQVAFHAVRQLARLAYGVATMRWGQSGFLSGPRGQTPRNLMGFKDGTNNPSI
                     AKPQLMNEFVWAGASADAPWMEGGTYTVVRRIRITLEHWDNTELDFQEQVFGRHKYSG
                     APIGKKNEFDAVDLKEEDKDGNPVIPENSHVRLSNQANNNGAQILRRSYSYNDGTNFY
                     IERWPPWRQETEYDAGLIFVAHQSDPRTGFIPINDKLAKFDMMNQFTTHVGSAVFAVP
                     QGAKRGSYIDAGLFET"
     gene            11798..12826
                     /locus_tag="VHV99_14770"
     CDS             11798..12826
                     /locus_tag="VHV99_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HEX3636718.1"
                     /translation="MAHSWFGTRLRLLSCAALTLAAFATVPSAAHAASITLYSAQHEQ
                     VVNLLAKDFEKESGISVKIRSGEGPAMAAQLVAEGAATPADVYFTENSPELVLLEEKG
                     LLSKVNDSTLATVPARFNSPSGVWVGVTARENVLAYNTNKVQASQLPQSLFDLAKPEW
                     KGKVGIAPSDGDFLLLVSAVLALKGEAQTVAWLKGLKANAQIFDDDEGVVAAVNRGGV
                     ATGLINNYYWARLHAELGDAKTRSAIYHFGNGDAGALVNVSGAAVLKSAHNTDGGQKF
                     LAYLVSERAQKLMANSHVMFEYPLHAGVAPDPILKPFTELNPPALTIQQLGDDSQAGK
                     LLRQAGLL"
     gene            12827..14431
                     /locus_tag="VHV99_14775"
     CDS             12827..14431
                     /locus_tag="VHV99_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX3636719.1"
                     /translation="MSEAVSAGPPAVIPAAVRARARAPRGLFAAAALSALLVLLPIAF
                     TFWRAASFGIGEAVDLIFRPLVGELLVNTLLITVSTTLVCAAVGTAAAWFVERTHLPG
                     RRVWAVLAAAPLAMPAFISSYAWVSLSLDLQDFNGALLVLSSAYFPLVYLPVAAALRS
                     MDPALEESARALGCNRWTTFIRVVLPQLRPALLGGMLLVALGVLSEFGAFTLLRFRTF
                     TTQIYAEYRTSFDGGGASLLACLLIVICLIVLAFEFRVRGAARYERVDRGTRRAVLRY
                     DLGAWRWIVVAGFAALAIATLGVPLGMIGYWLTQPGAAAVTPADVSPELLFDATLSSL
                     GFGLAAALLTTLLVVPLAFLLVRYPTRFATLFERTVFLAQGIPGLVIALAIVSLAVHA
                     LQALYQSATLLVIAYAMLFMPVALVSVRAAFMQAQPRLEETARALGLSWSQTLFRVVL
                     PLAGPGLGAAAAMVFISVVTELNATLLLSPIDTQTLATQVWADTSTMAFAAAAPYAAL
                     LTGISLCASGLLFALLGKSALLGERS"
     gene            14473..15531
                     /locus_tag="VHV99_14780"
     CDS             14473..15531
                     /locus_tag="VHV99_14780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017335205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3636720.1"
                     /translation="MSELRIRGLQKSFDGHPVLHGIDLSVERGTLLALLGPSGSGKTT
                     LLRLLCGFERADRGSVDIDGRRVAGDNLHMPSEQRRIGYVPQEGALFPHLSVADNIVF
                     GLPRTQRRARHRVAELLELVGLPANFGERAPQQLSGGQQQRVALARALAPSPTLVMLD
                     EPFSSLDAALRLETRQAVASALAATGATAVLVTHDQSEALSLGHEVAVLWNGRLIQTA
                     TPETLYRRPVTRELASFVGEAVLLPGVVKQDRVNCELGDLPLCEPMGNGAVDVMVRPE
                     QIRLLRAGEIMQNGVASYEAVVQDVIFQGQDAGVALQLQSGARTVVRARVPGYLSPRP
                     GENVRLAVDGEVTAYPRV"
     gene            complement(15646..16527)
                     /locus_tag="VHV99_14785"
     CDS             complement(15646..16527)
                     /locus_tag="VHV99_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX3636721.1"
                     /translation="MHPEFDVDLLRTFVAVVETGSFTKASTTVHRSQAAVSMQIKRLE
                     TMLGTTLFARNTRNLSLTRPGNTLLEYARRVLALHEEAWSAIVRPEVTGRVVLGAPDD
                     YVSSLLSPVLRRFSTLYPHVEIEIVCAQSTSLAPMLADNKIDLAFVTRDRKLRGEFVR
                     SEPMVWVGASADTPVLAASPLPVGLYEPGCVARQHTLAALDGARIRYRAAFSSASLMG
                     LVATVDAGLSVIALTRCSVPPRLAILGEAQGLPKIAPLEIVVARSAKSDRPTCDYLAA
                     QMVQDLSLRTPGRGATA"
     gene            16642..17562
                     /locus_tag="VHV99_14790"
     CDS             16642..17562
                     /locus_tag="VHV99_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX3636722.1"
                     /translation="MSLTQRQQGALTLASGGLLMGSLGIFVEEARLGALTLVFFRCLF
                     GFLSLAAYCAWKGFFTRAHFTRRTVALALISGVLMVTQWVGFFDAIHRTSIAVATVVF
                     HVQPFWVVLMGAALFNERLGVDRLGWIATAFVGLVLASGVAASENLQGHMSYLIGIGE
                     ALAGSVLYASVTLIAKGLGNLRPHLLTLAQCAVGVVCLPFIAPLTAVHIGPMQWFWLV
                     GMGVLHTGLSYVLIYGALPKLTTPVIAVLLFVYPLTAIVADAVVYGRALSVPQLGGMT
                     LIVIASLGVNLGWPLLSMLRPGGRARHHAD"
BASE COUNT         3281 a         5701 c         5512 g         3138 t
ORIGIN      
        1 cgcgattgac cacatcggcg gtcgcggtga gcacgtcgcg cgcggcgcca tggacatgcg
       61 gaatggtccg catgctcagc ggatcttgcg tgcgctgccc gaccaccgcg gcgagaatgc
      121 cgctatcggc aagcgcggta cgcatccgtt cgccgaccag attcaggccc ggcgaaatgc
      181 gcagcgccag cgagtcttca tcgaaggccg cgagctgtcc ccgcagattc tcgaagctca
      241 tcgcggcgac tacgtcggtc cagtcgagca gacgtgcggc gcgcgcgaga gccaatgcgg
      301 cgagacccgt cacgcacggc gtgccgttga tgaggctcaa gccctccttc gcttcgagca
      361 cgagcggttc gaggccgagc agttgcaacg cgtcacgccc tttaaggcgt tcgccgcgat
      421 agcgcacatg tccctcgccg atgcagacca acgcaatatg cgccatatgg ctcagataac
      481 cgaccgagcc gaacgccggc acttccggca ggcaatcggc attgagcaac gccaccagct
      541 gatccgcgac ttcgaggcga attcccgagt gcccgtgcgc aaaattgttg acggcggccg
      601 ccatgatcgc gcgcgtttcg gcgataccga gcggcgcgcc cacgccgacc gcatggctca
      661 tcagaatgtt gcgcgacagc gtgcgctgtt cggccggcga cacgatcaca tcgcacagcg
      721 cgccgacgcc ggtattcacg ccatacgcgc gaatcccgcg ctcgacgatc tgcacgacga
      781 gcgcacgcgc cgccgcgata cgcgagcggg cgtcggcgga aagctcgagc ggttcgccgg
      841 cggcgatagc cgccacctga gcccagtcga ggggacgatc ggaacggatc acggccatga
      901 tggtgctttg cccggtgaag tgacgaagtg gtgaacagcg cgtgctgttc ggttagttag
      961 tcgtgcgatc ctggtggctc gacacgaatt ggcggaagcg atccgacttg cattcgacga
     1021 acacttcgtc cggcgtgccg tcggcctcca cctgaccctg atgcaggaac atcacgcgat
     1081 tcgacacatg gcgcgcgaag cccatctcgt gcgtgacgac cagcatcgtg cggccttctt
     1141 cggcgagcga gcgcatcacg cgcagcactt cgccgaccag ttcggggtcg agcgccgagg
     1201 tgggttcatc gaacagcatc accttgggat gcatcgccaa ggcacgcgcg atcgccacac
     1261 gctgctgctg accgccggac aaatgtgccg gatagtgacc gcgcttttcc gcgagaccga
     1321 ctttggcgag cagcgcttcc gcttcttcga ccgattcggc gcggctgcgc ttttgtacgc
     1381 gcatcggccc ttcgatcaga ttctccagca cggtcatatg cgaccagagg ttgaagttct
     1441 gaaacaccat gccgagttgc gagcgcaccc ggtccacctg gcggcggtcg ctcggttgca
     1501 gcttgccgtc gccacggcgt ttcattttga gttcttcgcc gccgagcgcc accgagccgt
     1561 catccggcgt ttcaagcaga ttcaggcagc gcagaaacgt gctcttgccc gagccgcttg
     1621 cgccgagaat cgaaatcaca tcgccttgat gggcatcgag cgaaatacct ttgagcacgt
     1681 ggtggtcacc gaacgactta tggatgttct tgaccgacag tgcgacaggt gccgtagcgt
     1741 tcatgagatt ctccagattc tgcgggatgt gcgttcgttc agggtgcggc gcggcgtgct
     1801 tcggtcgtgc tggaaaccgg ccggttcggt gagctcgacg cgggcgccgc ccgcagatgg
     1861 cgcgacagac gcgtttcgag gaagccgagc aaacgcacga tgacgaaatt caggaacaga
     1921 tagatcaacg cggcgcagat gaatacttca gtggtgcgat aggtttgctg aatgatctgc
     1981 tgcgccacgc cggtcacttc ccacacggtg acaagactcg cgagcgcggt cgatttgacg
     2041 agcagcacgg cctcggtcga atacgcgggc agacattggc gcagtgcaat cggaccaatc
     2101 acacggcgca gcagcgcgaa acccgacagg ccgatcgaat agcccgcctc gatctgaccg
     2161 accggcacgg ccatcaaacc accgcggata atctcggcgg tgtatccggc ggtgcacagc
     2221 gccaacgata aaaccgcgca catgtagggc tcgcgcagca ccggccacag gaagctctcg
     2281 cgaatcacgc cgaactgacc catgccgtag tacacaagga acatctggat cagcaatggc
     2341 gagccgcgga acacgaggat gtaagcgcgt gcaaaacgat ttggcagcca atgcggcgag
     2401 acgcgcatcg tcacgatgac gagcgagagc aagccgccga ggatcagtga gcagaaaaac
     2461 agaccaaggg tggtcggcac cgccgcgatc agttgccgca gcgtgtcgag cagaaaatcg
     2521 aagtcgaaat gcatgatgtc cggtccccgt cagttgcgcg cgaagttgcg cttgaaggat
     2581 cggcccacgc gcgcttcggc gcggttgaag acccggttcg agatgctggt catcagcaga
     2641 tacagcgcgc cgcccaccac gaagaacgtg aagtactgat gcgtcgaacc cgccgccacc
     2701 tgactggcgc gcagcagttc ggcaagaccc gtgaccgaaa tcagcgccga atccttcagg
     2761 ctcaattgcc agacattgcc gatgccaggc aacgcgaatc gcaatacttg cggaatcaga
     2821 atgcggcgcg ccatggtcat ggtgggcatg ccgatcgagc gcgcggcttc gagttcgccg
     2881 cgcgataccg cgagcaccgc cgagcgatac acctcggcct gatacgcgcc tgaaatcatg
     2941 ccgaccgcga gcgcaccgat cacgaacggc ggcacgccga caaagccgtc cgcgccgaac
     3001 cactgaccga tcgtggtcac gagcgtcgag ccgccgaaat agaacaaata gatgacgagc
     3061 aactcaggca cgccgcgaaa cacggtggtg tacacgtcgc cgatcacgcg cagcgtgcga
     3121 aaccgcgaca gcttggccgc cgcgatcagc gcgccgaaca cggcgccgac cgcgagcgcg
     3181 gccagggtga gcgcaatcgt catcaatgcc gcgagcagca acacgccgcc ccagccttcc
     3241 ggcccgaagc cgagcatctc tatcagagcc attccctatc cctcctccat gacggcggat
     3301 cgaaccagcc ggcctgcacg cttctcacac cgcgcatgcc gctacagctg aacgctcagg
     3361 cgaatcaagg cgtgacgtcg gtcttgaacc acttggcgga gagcttcttg acggtgccgt
     3421 cggcaagcgc ggcgctgatc gcggtatcga acttcgcttt caggtcggcg tcctgcttgc
     3481 ggaatgcgag gccttcgccc ggaccccaga tcgggccgcc gatcttcggg ccggccatca
     3541 cgatcgacga cgtttccttc ttgtcgatgt tggcggcgta gtaggtcacg tcgtcgaacg
     3601 aggcgtcgat gcggccgttg gccagatcga ggtcacgctc aggcgaggtc ttgtagacgc
     3661 ggatcgtggc gacgtccttg aagccgtcgt tgatgaactt ggtgtagacc gtgcccgact
     3721 gaatgccgat ggtcttgccc ttcagctgtt tgcgcaaggc gtcgacggtg ggctggtcgg
     3781 ttttcggatc gcccgatagt ttgacgacgc ccgcggtcgg cacggctttc ggcagcactt
     3841 tcgcgtcggt cacggcgaac gtggccggcg tggcggcgta tggcttcgag aacgcgatga
     3901 tcttttcgcg ctccggcgtg atcgagatcg catccatcag gacgtcgaac ttgccggctt
     3961 gcagaccggg gatcatgccg tcccagtctt gcgcgacgag gttgcactgc agcttgatgc
     4021 gctcgcacag attggcgacc agctcaggtt cgaaaccgcc cagcttgccg cccggcaagg
     4081 tcaggttcca cggtgcgtaa ccgccttcca gcgcgatcgt cacggtcttc cagtctttgg
     4141 cctgcaacgg tgccgcgaag atcgtcgcgg tgccgaccac ggcgcccatc aatgcttttg
     4201 ctaacgtcgt gcgcttcata ttcacgtcga aactccttgg atcgaggaaa ccgtcgggct
     4261 ggatttttaa ccgccgttta atttgtatag acaagtctgc atgagtcaaa aagccctcgc
     4321 aagcggattt gttcaaaatc gcatggatca tcgggtaagc ccctacgcac cgaagcgcga
     4381 caaggcttat aagggagttt tgaaagcagg gagacaatgc ggcggacctc agcgcgtgcg
     4441 tcagcgtggt tcgctttgtc tagacaggtt ggtgtgggag gtgttgcaac acttcgcgta
     4501 gcggcagtgc gcgccctacc agataaacgg cacgaagcgg taacgcacgc gtgacgtgta
     4561 gtcggtataa ccgtcgagtt gctccgccag tacgcgctcc tcgcgcaccg cgcgccagcc
     4621 gataccgacg atcagcagcg gcacgcaggc aaaaccccac cacgagccca gcaacagcgg
     4681 cgtgccgacg aacagcagaa gcgcggcgga gtacatcgga tgacggacat gggcgtaagg
     4741 gcccgtgtcg atgactttgt gaccacggct tgcctgaatc ttcacgacgg gcgcggcata
     4801 gctattggcc cggaagacga tcagacacat ggaaagacag acgaagatgc agagcgagcc
     4861 gaagctcacc agccaaaccg gcaacggcgc cgaccagcga tagcgcatgg cgtcgagtcc
     4921 catcaggacg agccaggcgc accacattac cgcaacggcc accatgaaga cgcggtccca
     4981 gcggctctgc tgcgcctgaa cgatcggcgc gagacgttcg gcaagcaggc cgggatcgtg
     5041 gcgcgccagc cagaatccga tccatagact caggccgccg gtttcgatca gataccacca
     5101 gccggctggc cacgcgaagg tgcccgcggc gccgaacagc agcgcaccca tgcatgccag
     5161 ccagaccacc gtttgcagga tgaggcgcgt gatcatggtg cgtgcgtggc gctcgtcgat
     5221 ggtggttcgt gcctacgagg agttagctgt catcgtgggc gcccgctacc ttgtctcagg
     5281 cggaagccgc cgccggtgag cgcacgcgcc cgaatacttc cgccatgtcg atgtctcccg
     5341 tgttcagacc gaattgatcg cgtagacagg ttgcgagttc gtccgcgctt ttcagttgcc
     5401 gttcgctgat gacgtgcccg tccgccgcgc gttcgttcag ttgatcgttg agcagtgtca
     5461 ggcgcgattg cgccaggacc cggcaaacga tcagattgtt caggaagaca gaatccggcg
     5521 cggtcgaggt gtaccagttc gacgtctcat agtcgatcca ttcgaccggg tgcagatcga
     5581 aacggtagac gcgcccccag gtttcatccg gagattgcac ttcgaggtag agcgcgtcgt
     5641 gcgctgcatc ggcgaggcga aacgtgccaa gcggcgtgga ctgcgcggca cctgcgatca
     5701 ggcgcagcgg cgctgtcaac gtcacgctgc cgaaaccgac atcggcgatc catgcttcgt
     5761 tgttgatgtc gacacgcaag accatgtgcg tgcgcggggg gatctcggcg gattcgcggc
     5821 cccaaagcac tcgccctagc agaggcgtga cctcgaagcc caactgcatc agcacgttcg
     5881 cgaagagtgc gttctgctcg aagcaatagc caccgcgttt ttgcgtaatc agtttttgtt
     5941 cgaccgattc gtgatcgagt ttcaccgcgc gccgcgtgag cggattcagg ttctcgaagg
     6001 gaatcgttgc gggatgcagc tggtggagcg cctggagcac ttccaacgtt gcggcgcgag
     6061 gccctttgta gcctatgcgc gtgaagtagt tttccagatt cactgtgtgt gacatcggtg
     6121 tcgtcccggt atggattttg aacgctgggt actcgctcgg caatcatggg cctcgcgcta
     6181 cagcgtggct tcgggagatg atcatagcgt cttttgttgc ctgcccaggg cgtgggtcgt
     6241 tgcctgtttt tcgtcgtggc gcgtggtgat ctgttggcac cgcccctgtg ccgggcggcg
     6301 cctgcttcag agaacgctcg ctactgcccg cccttcaacg ccgacgtcgc gatcgacggc
     6361 acactgatcc catggctcga tgcgaactgc accgccgagc tcagcgtcga catcagcgac
     6421 tgcaattgcc ccggcgcgga ctgcgcgata tacgacgcgc tctgcgccgt ctgcgggttc
     6481 attcccgact gcaagagcga cgatggactc atctggccga tactccccag cccagactgc
     6541 aaactggaat actggctcac acctttgccc aacgacgaca agccgtccgt gaacgcctgc
     6601 ttgttcaccg gtgtggccga cgcgccaccg ctcgcgcggc tcgcgaacgc ctgggtcaac
     6661 gtctgcgata acgactgctg cgtgctgccc acctgggtca actggctaac cgagggcgtt
     6721 ccgccactca gcaatcccgc cgcctgttgc gcctgccccg cggcgctgct catgcccatc
     6781 gccgacgcaa gactcgattg gccgctcagc acctgctggt tcgcgcccac gtaggtctgc
     6841 aacaactgcg atatgccacc cgatgaagca gttgcgcctg catccgccga agtattgccg
     6901 ccgcctatgc ccaactgctt cagattcagc tgcgcgctcg acacgccact cacgagcaaa
     6961 ccgccgctcg ccagcagcgt aagcaaaagg atgcgtttgt tcatatctat ctcctgacat
     7021 tagcctttcg aggtactcgt gcccttcgac atcgctctcc ggccccggtt cgatacgacc
     7081 acagacatac tggaacatta catttgcatt cgattttatt tacatccaaa caacctttag
     7141 tagcagtgcg caaagcccga aattacaccc cggtaatatt gatgcgaatc gttctcattt
     7201 gtgttgacgc atctaattcg gatgcgtacg atctcgccat ctgccggtat ttggcctcgc
     7261 gaacaatgcg gcacatcgcc acgtggcaca gcgatggtta gatcagtcga tacaacacta
     7321 ataaggattt cgatgaagtt cgcccaatcc actgagcccg ccggtcgggc gcgttcgctt
     7381 cgcggctccg cgccgcgcct cggcaccaca cgccgcacgc gtttgccctc gatgcggcgt
     7441 gccgcgctct gcacagcttt cgcgtgggcc tccttgaacg ccggcgccgc catggccgaa
     7501 gccaatcccg atgccacccc ggaagcgccc gaagccgacc tgaacattca ggcgactcaa
     7561 acgaatcagt ggaccggctt ctggaatcgc cagacgatgc tcggcgacat cggcggcctg
     7621 cgcccctggc tcggcaaata cggcgtgacg ttcgccctta ccgagaccag tgaagtgctc
     7681 gccaacctcc gcggcggtct cgcgcgcggt gcggactacg acggcctgac gacagcggcc
     7741 ttgcaaatgg atacgcaaaa ggcgttcggt ttgccgggcg ggctgttcaa cgtcagcgcc
     7801 ttgcagatcc acggcgccaa tctcagcgcc aacaagctcg gcacgctgaa tacggcaagc
     7861 ggcatcgaag ccgatgacgc gactcgcctg tgggaactgt ggtatcagca gtcgtttctc
     7921 aacaagcgca tcgacgtcaa gatcggtcag caaagtatcg accaggaatt catcaccagc
     7981 tcgaattcgg cactgttcgt gaatacgatg ttcggctggc ctgccctgcc gtcctacgac
     8041 atgccgtccg gcggtcccgc ttatccgctg tccggtctcg gcgtgcgcgt gcgtggccag
     8101 atcacgtctt cgttgacggc gctcgccggc gtattcgacg gcgatccgct cggcaacaac
     8161 ccgaacaaca agagcggcac caacttcaac ctgcacaacg gcacactgtt catcggcgag
     8221 ttgcagtatg cgatcaacca gccggccgac ggcgaaatgg tcggcatgag cggcggcggt
     8281 ctgccgggca cctacaaact cggcgtctgg tacaacaacg gcagcttcgc cgatcagcgc
     8341 ttcgacaaca ccggcctctc gctggcgaat ccggcgagca ccggcgtgcc gcagaatcat
     8401 cacggcgact acagcttcta cgcggtcgcc gaccagatga tctggcgccc ggatccggac
     8461 gagccgcgca gcctcaacgt cttcgcgcgc gtgatgggcg cgccgggcga ccgcaacctg
     8521 gtgagcgtcg ccgccaacct cggcattgtg atgaaggcgc cgttcgccgg ccgcgacaac
     8581 gacagcgccg gcatcgcgct cacctacatc aagatcggca accacgtgaa tggtctcgat
     8641 caggataacc tggcattcag cggcggcccc tacggcgtgc gcaccagcga aaccacgctc
     8701 gaagcgacct accagtatca ggtcaatccc tggtggcagc tgcaagccga tgcgcaatac
     8761 acgttcaatg ccggcgccgg ccagaatccg aacgatccga cgcagccgct gcgcaacacg
     8821 tttgtcatcg gcgtgcggac caacattacg ttctgatgcc gttcgccatc accacacacg
     8881 ctcaagaccc atcgaatccc atgctttcga tcatgaccgc aactaccgct ttgtgcgcag
     8941 gcaccgaggc tcactcatga acaatcccac gcgcaagttt tcgccgcgcg ccgcgcgcgc
     9001 cttgatcgcc gcaaccgtca gcgccgccgc attcgcgaca gccggcgtcg cgcacgccga
     9061 gccgcaaggt tttctcgaaa ccgtcaagca tcacaccacg ctgatcaaca cggtgccggg
     9121 caacggcgac cagaatccgt acgcggtggt ggtcgcgccg gtcagcgcgg gcaccgtcaa
     9181 gcagggcgac gtgctggtcg gcaacttcaa taactcgact aacctgcagg gcaccggcag
     9241 cacgatcatc aactatcatc cggacaccaa acagatgacg gtgttcgcca cggtgccgcg
     9301 cgacctgaag gaatgcccgg gcggcatcgg cctctcgacc gcgatgacga tgctgaagtc
     9361 gggctacgtg atcgtcggga gcacgccgag caacgacggc accaccggca ccaaaggcgc
     9421 cggctgcctg atcgtgatcg atccgaacgg cacggtcacg tcgaccatca ccagcccgaa
     9481 catcaacgat ccgtggggca acatggcggt cgtcgacaac ggcaccagcg cgacgctgtt
     9541 cgtcagcaac gcgggcttcg gcgtgggcgg tgcgcaaggc aatccgccgg tgttcaagca
     9601 ggccaccgtg ctgcgcctgg acctcgacgt gccggccggc aagccgcccg tcgtgaagaa
     9661 ggaaactgtg gtcggcagcg gctttggcgc gcaggccgac aagggcgtgt tcctcgtcgg
     9721 cccgaccgga ctggcgctct cgaacgatca gaaactgctg tacgtgtcgg acgcgatcgg
     9781 caaccgcatc accgaaatcg acgatccgat gacgcgcgac accagcgcgg gcgtgggtcg
     9841 ccaactgacg gccgatggcc tgctgcatcg cccgctcgcc atggtcaccg cgccggacgg
     9901 ccacctgctc gtcacgaacg cgttgaacgg ccagatcgtc gaaatcgatc ctgccagcgg
     9961 caagcagatc tacgcacgct ggatcgacac cgacaaggcg caatcgccgc cgggcaacgg
    10021 cgacctgttc ggtctcgcga tgacacccga aggcgacggt ttttattatg tgcaggacga
    10081 cgtgaacacg ctggttctgg ccaagtaggt gacgcaagtt cgtgacgcaa actcgtgccg
    10141 caaatcagcg acgcaaatgc gtgacggaag cagcgaagcg aatcaacgaa ccgaaccagc
    10201 gacccgcacc cggccccaga gccggtgcaa atccggcata tcccacggcc aactgaaggt
    10261 gaagtaagca tggcaaacga tcaacccccg cggccatcac ggcgcggctt tctcaaggcg
    10321 ggcggcgcag cggtagcggc cggcgcgagc ctcggtgcag gccttgcgac gtcgcagacc
    10381 gcccaggccg cgctcgccag aacgccggcg caccacgccg atccgcaaac ggcggtcgag
    10441 ccgttctacg gcttgcatca aggcggcatt gtcacgccgc agcagagtca cacgtacctt
    10501 gccgcgctcg atctgacgac cgacaaacgc gacgacgtga tcgccctgct gcgcacctgg
    10561 acggacactg ccgcacgcct cacgcagggc gccacggccg cgccgctgcc gaccggcacg
    10621 gcggccaaag cggaactggt ggacagcaag attgatacgc aggtcgacac gaagaccgat
    10681 cccaaggtcg acaacacggc ctacagcccg accaacacca aagccgcacc cgattccggc
    10741 gacgtgctcg gcctgggccc ctgcggcctg accatcacct tcggtttcgg tcccggtctc
    10801 ttcacgcatg aaggcaagga ccgctacggg ctggcctcgc gccgccccgc cgcgctcgtc
    10861 gatctgccgc gcttcaacgg cgaccagttg atcgctgaga aaaccggcgg cgacctgttc
    10921 attcaggcct gcgcaaacga ccagcaagtc gcgtttcacg cggtacgcca actggcgcgc
    10981 ctcgcgtacg gcgttgcgac gatgcgctgg ggccagtccg gtttcctgtc cggtccgcgc
    11041 ggtcagacgc cgcgcaatct gatgggtttc aaggacggca ccaacaatcc gtcgatcgcg
    11101 aagccgcagt tgatgaacga attcgtctgg gccggggcca gcgccgacgc gccgtggatg
    11161 gaaggcggca cctatacggt cgtgcgccgc atccgcatca cgctcgaaca ctgggacaac
    11221 accgagctcg atttccagga gcaggtgttc ggccgtcaca aatatagcgg cgcgccgatc
    11281 ggcaagaaga acgaattcga cgccgtggac ctgaaggaag aggacaagga cggcaatccg
    11341 gtgattccgg agaactcgca cgtgcgtctg tcgaatcagg caaacaacaa cggtgcgcag
    11401 attctgcgcc gttcgtactc gtacaacgac ggcacgaact tctacatcga gcgctggccg
    11461 ccgtggcgcc aggaaaccga atacgacgcg gggctgattt tcgtggctca ccagagcgac
    11521 ccgcgtaccg gcttcattcc gatcaacgac aagctcgcga agttcgacat gatgaaccag
    11581 ttcacgacgc acgtcggcag cgcggtgttc gccgtgccgc agggcgcgaa gcggggttcg
    11641 tatatcgacg cgggactgtt cgagacgtaa tgcaggccag gaagtaaacc gccgggccgc
    11701 aaggcaagcg gcaaacggca agcggcaagc ggcaagcggc aagcggcaag caacttatca
    11761 aagatcgaca acaccatcac taacggattt tagaatcatg gctcattcct ggtttggcac
    11821 ccgcctgcgc ctcctcagct gcgcagcact cacgctggct gcgttcgcaa cggtgccgtc
    11881 ggcggcccac gctgcatcga tcacgctcta cagcgcgcaa cacgagcagg tcgtcaatct
    11941 gctggcaaaa gacttcgaaa aagagtcagg catttcggtg aagatccgca gcggcgaagg
    12001 cccggccatg gccgcgcaac tcgtcgcgga aggggccgcc acgcccgccg atgtctactt
    12061 cacggaaaac tcgcccgagt tggtgctgct cgaagagaaa ggtctgctga gcaaagtgaa
    12121 tgactcgacc ctcgccaccg tgccggcgcg cttcaattcg ccgagcggcg tgtgggtcgg
    12181 cgtgaccgcg cgtgaaaacg tgctggccta caacacgaac aaggtgcagg catcgcaact
    12241 gccgcagtcg ctgttcgatc tggccaagcc ggaatggaaa ggcaaagtcg gcattgcacc
    12301 gagcgacggc gacttcctgc tgctcgtgag cgccgtgctc gcgctgaagg gcgaagcgca
    12361 aaccgtcgca tggttgaagg gcctgaaagc caacgcgcag attttcgacg acgacgaagg
    12421 cgtggtggcc gccgtgaatc gcggcggcgt tgcaacgggt ctgatcaaca actactactg
    12481 ggcgcgtctg cacgctgaac tcggcgatgc gaaaacccgc agcgcgatct atcacttcgg
    12541 taacggcgac gccggtgcgc tggtgaacgt gtcgggcgca gcggtgctga agtccgcgca
    12601 caacacggac ggcggccaga agtttctcgc gtatctggtg agtgaacgcg cgcagaagct
    12661 gatggcgaac agccacgtca tgttcgaata tccgctgcat gccggcgttg cgccggaccc
    12721 gatcctcaag ccgttcaccg aactgaaccc gccggcactg acgatccagc aactcggcga
    12781 cgacagccag gccggcaagc tgctgcgcca ggcaggcttg ctgtaaatga gcgaggccgt
    12841 gtcagccggg cctccggctg taatccccgc cgcggtgcgc gcccgtgcgc gcgcgccgcg
    12901 cggtctgttt gcggcggcgg cactcagcgc tttgctggtg ttgctgccga ttgcgttcac
    12961 cttctggcgc gcggcgagct tcgggatcgg cgaggcggtc gatctgatct tccggccgct
    13021 ggtcggcgaa ctgctggtca atacgctgct gatcaccgta tcgaccaccc tcgtgtgcgc
    13081 tgccgtcggt acggcggccg catggtttgt cgaacgcacg catttgcctg ggcgtcgcgt
    13141 ctgggccgtc ctcgctgccg cgccgctggc catgccggcc ttcatctcga gctatgcgtg
    13201 ggtgtcgctg agcctggatc tgcaggactt caacggcgcg ctgctggtgc tgagctccgc
    13261 gtatttcccg ctcgtttatc ttccggtggc cgccgcgtta cgcagcatgg acccggcgct
    13321 cgaagaaagt gcgcgcgcgc tgggctgcaa tcgctggacc acgttcattc gcgtggtgct
    13381 gccgcaactg cgtccggcgc tgctcggcgg catgctgctg gtcgcgctcg gcgtgctgtc
    13441 tgaattcggc gcgtttaccc tgctgcgctt tcgcaccttc accacgcaaa tctacgcgga
    13501 ataccgcacc agtttcgatg gcggcggcgc ttcgctgctc gcgtgcctgc tgatcgtgat
    13561 ctgcctgatc gtgctggcgt tcgagtttcg cgtgcgcggc gcggcgcgct acgaacgcgt
    13621 ggatcgcggc acacgccgcg cggtgctgcg ctacgacctg ggcgcatggc gctggatcgt
    13681 cgtcgccggc ttcgcggccc tggcgatcgc gacgctcggc gtgccgctcg gcatgatcgg
    13741 ctactggctc acgcaaccgg gcgcggctgc cgtcacaccg gccgacgtgt cgcccgaact
    13801 gctgttcgac gcgacgctct cgtcgctcgg tttcgggctc gccgccgcgc tgctgaccac
    13861 gctgctggtc gtgccgctcg cgttcctgct ggtgcgctat ccgacgcgtt tcgcgacgct
    13921 gttcgagcgc accgtgtttc tggcgcaagg cattccgggg ctggtgatcg cgctggccat
    13981 cgtgtcgctc gcggtgcatg cgctgcaggc gctttatcaa agcgcgacgc tgctggtgat
    14041 cgcctacgcg atgctgttca tgccggtggc gctggtgagc gtgcgcgccg ccttcatgca
    14101 ggcgcagccg cgcctcgaag agactgcacg cgcgctcggc ctgagctggt cgcagacgct
    14161 gttccgggtg gtgttgccgc tggcaggccc tggactcggc gcggcggcgg cgatggtgtt
    14221 catctcggtc gtcaccgaac tgaatgcgac gctgctgctc tcccccatcg acacgcaaac
    14281 gctcgcgaca caagtctggg ccgacacgtc gacgatggca ttcgccgccg cggcgcccta
    14341 tgcggcgctg ctcaccggca tttcgctgtg cgcctccggc ctcctgttcg cgttgctcgg
    14401 caaatcggcc ttgctcggcg agcgcagttg aacgccgcgt aacatcgccc tgttccttga
    14461 catcggattt tcatgagcga acttcgtatc cgcggactgc aaaaatcgtt cgacggccat
    14521 ccggtgctgc atggcatcga tctctccgtc gagcgcggta cgctgctcgc gctgctcgga
    14581 ccgtccggca gcggcaagac caccttgctg cggctgttgt gcggtttcga acgcgcggat
    14641 cgcggcagcg tcgacatcga cggccgccgc gtggccggcg acaacctgca catgccgtcc
    14701 gagcagcgcc gcatcggcta cgtgccgcag gaaggcgcgc tgtttccgca tctgtccgtg
    14761 gcggacaata tcgtgttcgg cttgccgcgc acgcagcgcc gagcgcgcca tcgggtggcc
    14821 gaattgctgg agctggtcgg cttgccggcg aacttcggcg agcgcgcgcc gcaacagtta
    14881 tcgggtggtc agcagcagcg tgtcgcactg gcgcgtgcgc tcgcgccgtc gcctactctg
    14941 gtcatgctcg atgagccctt ctcctcgctt gacgccgcgt tgcggctcga aacgcgacag
    15001 gcggtggcga gcgccctggc cgcgacgggg gcgactgccg tactcgtcac gcacgatcaa
    15061 tcggaagcgc tgtcgctcgg tcacgaggtc gcggtgctgt ggaacggccg gctgatccag
    15121 accgcgacgc cggagacgct gtaccgccgg ccggtgacgc gcgaactcgc gtcgttcgtc
    15181 ggtgaagcgg tgttgttgcc gggggtggtc aagcaggatc gcgtgaactg cgaactcggc
    15241 gatttaccgc tgtgcgagcc gatgggcaac ggcgcggtcg atgtgatggt gcggcctgaa
    15301 cagatccgcc tgttgcgcgc gggagaaata atgcaaaacg gcgttgcgtc ttatgaagcc
    15361 gtcgtgcagg acgtgatctt tcaggggcag gacgcgggcg ttgcgctgca attgcagtcc
    15421 ggcgcgcgga cggtggtgcg cgctcgcgtg cctgggtatc tgtcgccgcg gccgggcgaa
    15481 aatgtgcggc ttgccgtgga tggcgaggtg acggcttatc cgcgagtttg aagcggcgta
    15541 atcaagctgc gagatcgagc ctcacatcaa gccgcgcgtt taaaccacac agtcaatcag
    15601 cgcggctgag caacgccctt ttagggcatg cccatcgcgg catcatcacg ccgtcgcccc
    15661 acgccccggc gtgcgcagcg acaaatcctg caccatctgc gcggccagat aatcgcacgt
    15721 aggacggtcc gatttggcgc tgcgcgccac cacgatttcc agcggcgcga tcttcggcaa
    15781 gccttgcgct tcgccgagaa tcgcgagccg cggcggcacg ctgcaacgcg tgagcgcgat
    15841 caccgaaagg cctgcatcca ccgtcgccac caagcccatc agactggcgc tgctaaatgc
    15901 cgcccgatag cggatacgcg ctccgtcgag tgccgcgagc gtatgctggc gcgcgacgca
    15961 gcccggctcg taaagcccaa ccggtaaagg cgacgccgcc agcaccggcg tatccgccga
    16021 tgcccccacc cacaccatcg gttcgctgcg cacgaattca ccgcgcaatt tgcggtcgcg
    16081 cgtgacgaag gcgagatcga tcttgttgtc ggccagcatg ggcgcgagcg acgtgctctg
    16141 cgcgcagacg atctcgatct ccacgtgcgg atacagggtc gagaagcgtc gcaacaccgg
    16201 cgacagcagc gacgacacgt aatcatccgg tgcgcccagc accacccgcc cggtcacctc
    16261 gggacgcacg atcgccgacc acgcctcctc atgcaacgcc agcacgcgcc gcgcgtattc
    16321 gagcagggta ttgcctggcc gcgtaagcga gagattgcgc gtgttacggg caaacagcgt
    16381 ggtgccgagc atggtttcga gccgtttgat ctgcatgctg accgccgcct gcgagcgatg
    16441 cacggtggtc gacgctttcg tgaagctgcc cgtttccacc accgcgacga aagtgcgcaa
    16501 caaatcgacg tcgaattcgg ggtgcatgat ttatcaatcc agcttatgga attgctcaat
    16561 ttaattcgtt tgtcggtgcc gtgcaagccg cgcaatactt gtgactcccc tttacgtgac
    16621 ccattttcgg agccgcttcg catgtccctc acccaacgtc aacaaggcgc cctcacgctg
    16681 gccagcggcg ggcttctgat gggctcgctc ggcatcttcg tcgaggaagc gcggcttggc
    16741 gcgctgacgc tggtgttctt tcgctgcctg ttcggcttcc tctcgctcgc cgcctactgc
    16801 gcgtggaagg gcttcttcac gcgcgcgcat ttcacgcggc gcaccgtggc gctggcactg
    16861 atttccggcg tactgatggt cacgcaatgg gtcggcttct tcgatgccat tcatcgcacc
    16921 agcattgcgg tggcgaccgt ggtgtttcac gtgcagccgt tctgggtcgt gctgatgggc
    16981 gcggcgttgt tcaacgagcg cctcggtgtc gaccggctcg gttggatcgc aaccgcattc
    17041 gtcgggctcg ttttggcctc gggtgtcgcg gctagcgaga atctgcaggg gcatatgagc
    17101 tacctgatcg ggatcggcga ggcattggcg ggttcggtgc tgtacgcgag cgtcacactg
    17161 atcgccaaag gactcggcaa tttgcggccg catctgctga cgctcgcgca atgcgcggtc
    17221 ggcgtggtgt gcctgccgtt cattgcgccg ctcactgccg tgcatatcgg gccgatgcag
    17281 tggttctggc tggtcggcat gggcgtgctg cacacggggc tgtcgtatgt gctgatctac
    17341 ggcgcgctgc cgaagctgac cacaccggtc atcgccgttt tgctgttcgt ctatccgctg
    17401 acggcgatcg tggccgatgc cgtggtgtat gggcgggcgc tgtcggtgcc gcagctcggg
    17461 ggcatgacat tgatcgtgat tgcgagcctc ggcgtcaatc tcggctggcc gctgctgtca
    17521 atgctgcgtc cgggcggacg ggcgcggcat cacgctgatt gagagtccgg cgggcgggaa
    17581 tcgaactgag cccgcccggt tctttgtttc agtcgtcgta gtggaaacgg ca
//
LOCUS       DATASH010000534        17653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4042183,
            whole genome shotgun sequence.
ACCESSION   DATASH010000534 DATASH010000000
VERSION     DATASH010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 17653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17653
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4042183"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VHV99_14795"
     CDS             <1..262
                     /locus_tag="VHV99_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636723.1"
                     /translation="HRHGSNPVGAAITDALVKESAKLDPALPPPTQAIQPLQPTQAAQ
                     PSLDDAYHRGSNPVAAAMTEQLVRESAKLDPGLPPPNQASMK"
     gene            complement(287..799)
                     /locus_tag="VHV99_14800"
     CDS             complement(287..799)
                     /locus_tag="VHV99_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3455 domain-containing protein"
                     /protein_id="HEX3636724.1"
                     /translation="MLRNISQASFAIVTASLLAACATPPASPPTVSLDPPQAERVMTL
                     AASGVQNYSCEFDAQQRLGWVFKSPQATLYDASGHAAVRHGAGPSWEAEDGSRIVGRV
                     LAQQPSETRASIPQLLLETHSTAGTGTLSAVRYVQRLHTVGGLAPTAPCSTEHQAGSS
                     PYLADYVFYR"
     gene            911..2062
                     /locus_tag="VHV99_14805"
     CDS             911..2062
                     /locus_tag="VHV99_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014252556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HEX3636725.1"
                     /translation="MSDQQSYACVCCSDSQSAAFSRRRFLTLAAESAAALALFPGLAY
                     AESVPSIAYESVPDFLRLPRDVYFGECSGVSFNSKGHIFVLSRGNSIGPAYGAAAAQL
                     LEFAPDGRFVGEIGHNLYAWSFAHTVKVDRHDNIWVTDKGSDMVIKFTPEGRVAMVFG
                     RKQEASDEETAPLKHPNPPLPSEPGRFRQVTDVAWDSAGNTYISDGYINSRVAKVDKD
                     GNWLKSWGDRGTGPGQFHTPHSIAVDAKGLVYVADRSNRRIQVFDGEGNFQRQFTIDV
                     PVPPGAQPAIGNVPDEATIAAGTFAPGSPWAICISPGPNQVLYSSDAFPGRIYKLTLE
                     GKLLGVLGQSGKQLKQFGWIHQMACPSENVLYVAELLNWRVQKLMLRPS"
     gene            complement(2093..3394)
                     /locus_tag="VHV99_14810"
     CDS             complement(2093..3394)
                     /locus_tag="VHV99_14810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX3636726.1"
                     /translation="MTPGRRRLLIAGAILLIVALGWGISAIVEPAFRHTIVITTGADN
                     GIYRGFADRYAPVLKRDGITLDIRGSSGSSENYQRLKDPDSAYEVGFIQSGTTSPKET
                     DHLQTIAAVSYEPIWVFYRGGSADSTINRLAQLRGKKISIGVPGSGLLNVSQLLLGYS
                     GVTRDNTTLLEMDATKAYQALENGQLDAAFFIGRPDAPMQTTLLNSNLKLMSFAQADA
                     LVQKFPSLSKVIFPRAATSIANDLPQNDVTLLAATALLVAKDTLHPALVYLLLDAAKT
                     VHGGEDYFTPLGVFPNLKTEEFPVSEESERYFKTGRPFLQRYLPFWLASFIERRLLIL
                     LPFMALLLGLLQALPRMVETRIKKRLVVWYREIKALEDEIWKNSHPDADQIAQWKDEI
                     EHIDAHANRIRMPQRYFQDVYALKQAIAVVRNRIYRVAGEQ"
     gene            complement(3584..4816)
                     /locus_tag="VHV99_14815"
     CDS             complement(3584..4816)
                     /locus_tag="VHV99_14815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX3636727.1"
                     /translation="MKFDAVVLGAGIVGVCVAVHLQKRGRQVALVDRKLPGNETSFGN
                     AGLIQRESVYPYAFPRDLGTLLRYARNQSLDVRYHADAILKTAPFLWQYWRNSHPAKH
                     AAIAKSYSTLIEHCVDEHRALAAAAGASALLRPTGWIKVFRTEPVRDAETRIAERWHR
                     EYGVEFEALDASGLQQKEPDLDKSLPGGLRYTASDSVNDPNALITAYAKYFEALGGRF
                     FIGDADSFREGWAVDTEVGTIAASSAVVALGPWSDRVSSRLGYRLPLAVKRGYHMHYA
                     SEASARLNHPVLDVENGYLLAPMAKGIRLTTGAEIAHCDAPKTPTQLAAVEPIARTLF
                     PLGQRLDDEAWMGRRPCTADMMPIIGPATHHKDLWFAFGHAHHGLTLGPVTGRLIAEM
                     MTHEETLVDTRPFRVERF"
     gene            complement(4965..5636)
                     /locus_tag="VHV99_14820"
     CDS             complement(4965..5636)
                     /locus_tag="VHV99_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine cyclotransferase"
                     /protein_id="HEX3636728.1"
                     /translation="MNRSAARIVREYGPFPGVDKVHGVTYDGQHVWIATGDKLNAFDP
                     VSGETLRSIDVAAQAGTAFDGQHLFQIAGDLIQKIDPDSGRVLATIPAPGGGSGNSGL
                     TWAEGTLWVGRYQDRKILQVDPETGAILRTIASNRFVTGVTWNDGELWHATWEDDESE
                     LRHVDSRTGEVLESLEMPPGVYVSGLESDGGDQFFCGGGTSGKVRAVRRPRRDAQAPV
                     ESANE"
     gene            complement(5650..6867)
                     /locus_tag="VHV99_14825"
     CDS             complement(5650..6867)
                     /locus_tag="VHV99_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3636729.1"
                     /translation="MDSLVTAAARALAAGDPLGALNRVALRDDAPALALRGIAMAQLG
                     DLVRAKALVRSAARAFGPKEPVARARCVVAEAEIALASRDLGWPAKALDAARATLEAH
                     GDRLNAAHARYLEVRRLLLIGRLDEAGRLLARLDPAPFPPPLRTAHELVVAGLAMRRL
                     QTNTARSALARAERAAYHAGIPSLTAEVESARLLLNTPAARLIAHGEERLLLLEEVEA
                     LLASKALIVDACRYVVREARIVVPLARRPVLFALARALAEAWPEDVSRDALIARAFRT
                     KHPDDSHRARLRVEIGRLRALLGTLAGVNATKRGFALIPHGAREVVVLARPVEEPHAM
                     VLAFLADGESWSSSALAIALDASQRTMQRALDALAAGGKVQSFGRGRARRWMTPPMPG
                     FATTLLLPAPLTE"
     gene            7000..7764
                     /locus_tag="VHV99_14830"
     CDS             7000..7764
                     /locus_tag="VHV99_14830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006026088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HEX3636730.1"
                     /translation="MTTHTTGTREAWLAARLELLKAEKALTRQSDELVQQRQALPWVR
                     VDKAYRFETAAGSASLAELFKGRSQLLVYHFMFGPDYKAGCPSCSSIADGFDGIVVHL
                     ANHDVTLMAVSRAPLEKLQTYQRRMGWTFPWASSAGSDFNFDFNIAFTEAQQREGRIE
                     YNYERGGHAMDETQVPAPVAQFAATCGTDAATFTRDRPGLSAFVLEDGVVYHTYSTYA
                     RGVDGIWGMYQWLDRAPKGRNETGVWWKRHDEYEQR"
     gene            7806..8645
                     /locus_tag="VHV99_14835"
     CDS             7806..8645
                     /locus_tag="VHV99_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HEX3636731.1"
                     /translation="MSGRSIDARVSQRTFFGVSALLFVVSTGVTIAGAASMSAMGEMP
                     MPGGWTMSMTWMRMCGQTWPGVAASFLGMWDVMMVAMMLPSLVPMLWRYREAVGGICE
                     VRLDWLTALVGVGYFFVWSAFGMAVFSLGAALAALEMELPALARAVPLAAGVIVLSAG
                     ALQFTAWKARRLACCRAAPGGCLVVPVTLALPALPALPADAALAWRHGLRLGLHCCYC
                     CAGLTMVLLVLGVMDLRVMAVVTAAITVERLAPRGERVARVIGVGVVGAGLVLIARAV
                     GLG"
     gene            complement(8831..9148)
                     /locus_tag="VHV99_14840"
     CDS             complement(8831..9148)
                     /locus_tag="VHV99_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HEX3636732.1"
                     /translation="MKCPVCKTTDLLMSERRSIEIDYCPVCRGIWLDRGELDKLIALD
                     AGTADAAASARVTRESHRESPRDGHRDRDHAYENNRTHDDRRPDYRGHKKKRSIFDVF
                     DFD"
     gene            complement(9288..10037)
                     /locus_tag="VHV99_14845"
     CDS             complement(9288..10037)
                     /locus_tag="VHV99_14845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018434182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX3636733.1"
                     /translation="MDALLILIADPAAWAALLTLVVMEIVLGIDNLIFISILSNKLPE
                     AQRARTQRMGIMLALGLRLGLLGTVAWIAQLTAPAVALFGHAFSWRDLILLGGGLFLV
                     WKATSEIHHHVVHSEEARDKANTTVQLTALAAIGQILVLDIVFSVDSIITAVGMTEHL
                     PIMFIAVIAAVMVMLFAASPLSRFIDRNPTVVMLALGFLLVIGMTLIAEGFGSHVPKA
                     YIYAAMAFSAFVEALNMLVRRAKSKRPINAE"
     gene            10159..11040
                     /locus_tag="VHV99_14850"
     CDS             10159..11040
                     /locus_tag="VHV99_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3636734.1"
                     /translation="MLNYRHLYYFWIVVKEGGFARAAERLDMAVQTISAQVRELEKSI
                     GRQLLKPAGRGVTMTEAGETAFNRAEQIFQIGEALLDEMREAGGERVARLAVGLSDGI
                     SKLAAHALLAPVLGTPSLRLLCHEGEPAQLLSELALHRLDLVLACQPAPHNADLRVVS
                     QRLVGSPVDWYGPAEIVRKSARAGFPQCLADLPVLLPTGHGALRARLDRWFEAAGIRP
                     RIVGEFEDSALMAVFAARGLGVFPLAELGAEDVALLRRLRWLGRAEGVVDEIHAIRSR
                     RGQHHALASQVVAGARS"
     gene            complement(11058..11465)
                     /locus_tag="VHV99_14855"
     CDS             complement(11058..11465)
                     /locus_tag="VHV99_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HEX3636735.1"
                     /translation="MRTYPRNSPQAAARIVALALTSDGHVSSAEERALEGLDIADELG
                     LAPAQFARIVQALCEDQSIAHTPFAPPVGHLDAALLATLIDEIDDPALRRKVIRLCIA
                     VTIADDYLADGEIALLAAVFNAWGSAPARAASR"
     gene            11689..12021
                     /locus_tag="VHV99_14860"
     CDS             11689..12021
                     /locus_tag="VHV99_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004542952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HEX3636736.1"
                     /translation="MIQPSNVFKDNLAQLPAIDGIERIDLVDGKGAVVASIENKPGKQ
                     GSLAVYHYLKQAFGTLDAKAAEHGLLVFAEHTADARNRPGAHPNVDRLLAIVAGGEAL
                     RIDVVAAG"
     gene            12163..13185
                     /locus_tag="VHV99_14865"
     CDS             12163..13185
                     /locus_tag="VHV99_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006396842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX3636737.1"
                     /translation="MKQLQLGKTGPQSAAIGLGCMGMSGMYGPSDRAESIATIHAALE
                     AGITLLDTGDFYGSGHNEILIGEALKSAPPSRRDAAIISVKFGAMRDPAGGWSLYDAR
                     PAAVKNFLAYSLQRLGVEHIDIYRPARLDPNVPIEDTVGAIADMIKAGYVRHVGLSEV
                     GATTLRRAAAVHPVSDLQIEYSLISRGIEDEILPACRELGIGVTAYGVLSRGLISGHW
                     RKDAAGKGDFRAMSPRFQGDNIDHNLALVEALRKVADAKGVSVAQIAIAWVAAQGSDI
                     VPLVGARRRDRLAEALGAVDVTLTADDLAEIARAVPIGAAAGDRYAAAQMAHLDSEKG
                     SHGHAA"
     gene            complement(13314..15104)
                     /locus_tag="VHV99_14870"
     CDS             complement(13314..15104)
                     /locus_tag="VHV99_14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEX3636738.1"
                     /translation="MSLDTHKRDFSTNARLPGISALAAGIGVLSTLAAFVLLSLIHLF
                     TNLFIFQQFSFADRSPAVNTLGIWVVIIPVIGGLIVGFMARYGSEKIRGHGIPEAIEA
                     ILFGKSRMSPKVAILKPLSSGIVIGSGGPFGAEGPIIMTGGALGSLIAQCVKVTSAER
                     KTLLVAGAAAGMTAVFGTPVAAVLLAVELLLFEWRPRSFLPVALACAVAGFARAAFFG
                     TGPLFPLETAPPGAMSLVSCVIAGLMSGALASGLSAALYKTEDLFGKLPIHWMWWPAL
                     GGLVVGIGGFFEPRALGVGYDVIGDLLHQHIAIQIALAILVVKAIIWVVALGSGTSGG
                     VLAPLLMLGAGLGVVFGHVLPGGEPALWPLVCMAATLGATLGAPLTAIVFAFGLTHDS
                     NALLPLLAATLVAHGFATVVMRRSIMTEKIARRGYHIYREYGVDPLERHHVDEVMSRT
                     VETIDGDLSVGEALETYFGTTQTHRAYPVVRQGVLAGVVDRAVLERVRDDETGHALDS
                     RLADVLRHRSPAFALPSETCRLVATRLAVHGLERLPVVADAEALQLVGIVSRSDLVKP
                     SLAHFDEEHKKERFRRLRVHTGKRHFPPVP"
     gene            complement(15111..15524)
                     /locus_tag="VHV99_14875"
     CDS             complement(15111..15524)
                     /locus_tag="VHV99_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007747257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX3636739.1"
                     /translation="MKTLARNLTKADFEQLSEFRYQMRRFERFSEQAAQGEGITPLQY
                     LLLLHIKGYPERDWATVGELAERLQAQHHGVVALVSRCEALDLVRRQVSETDRRQVEV
                     HLLKAGEQVLARLAEMHRAELKSLKGAFNVPQIDY"
     gene            complement(15615..16775)
                     /locus_tag="VHV99_14880"
     CDS             complement(15615..16775)
                     /locus_tag="VHV99_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HEX3636740.1"
                     /translation="MSRDALIRWFSSFAPHPIAVRWPERFRSCLGALLGIAFTGGTMH
                     VLLGPAANIPLLVAPMGASAVLLFAVPASPLAQPWSIIGGNIVSATVGVACASWIADP
                     VGAAALAVALAICAMFALRCVHPPSGAVALTAVLGGPAVHALGYRFVAEPIAIQSLML
                     LCAAVIYHAATGHRYPHVAQNSEKGTAAAVSNEGFTRSDLEAVLNRRGELLDIGTDDL
                     ESLLRDVQLQAYARTFNELTCADIMSRSLVAVSAVTRASAAWSLLKQRHLKALPVTDE
                     KQHVIGIVTRADLVDKRAFSRGAGLSSPLQRWVRRSITPAPLVGALMNVDVHTVDATT
                     PIVELVPMFANYGHHHIPVLDSKRRLAGMITQADLIAGLYRQAYAQQQQRAA"
     gene            complement(16945..17376)
                     /locus_tag="VHV99_14885"
     CDS             complement(16945..17376)
                     /locus_tag="VHV99_14885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007746136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX3636741.1"
                     /translation="MQTAIDFEQLVNAIGDAIIMSDAGGSITLWNPAAERMFGFTQSE
                     ALGKSLDLIIPERLRGRHWDGYHKTMATGETSYGNDVLRVPALHKDGRALSIAFTVAL
                     LHSPQNELTGIVAVIRDETSRFQEDRLLRKRVAELEASARA"
     gene            complement(17485..>17653)
                     /locus_tag="VHV99_14890"
     CDS             complement(17485..>17653)
                     /locus_tag="VHV99_14890"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178896.1"
                     /note="catalyzes the formation of oxalyl-CoA from oxalate
                     and Formyl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HEX3636742.1"
                     /translation="KKRGSYLTVGSPIKFSDMKPEVTASPLLGEHTDEVLASLGYSRQ
                     DIFNLHEIKAV"
BASE COUNT         3275 a         5427 c         5723 g         3228 t
ORIGIN      
        1 gcatcgtcac ggctcgaatc cggtcggggc tgcgatcacg gatgcgctgg tcaaagagtc
       61 cgccaaactc gatccggcac tgccgccgcc gacccaagcc attcaaccgc tccaaccgac
      121 tcaagcggcc cagccgagcc ttgacgatgc ataccatcgc ggctcgaatc ccgttgcggc
      181 cgccatgact gaacagctgg tcagagaatc cgcgaaactc gatccgggac tgccgccgcc
      241 gaatcaagcc agcatgaagt agacgcgcga cgcgaagcgt gggctactac cggtagaaca
      301 cgtagtccgc gaggtaaggc gaactgccgg cttgatgttc ggtcgagcac ggtgccgtcg
      361 gcgctaaccc gccgacggta tggagacgct gcacataccg caccgctgaa agtgtgccgg
      421 tgccggccgt gctgtgcgtt tcgagtaaca gctgggggat gctcgctcgt gtttcgctcg
      481 gctgctgtgc gagcacgcgg ccgacgatcc ggcttccatc ttccgcttcc catgacggtc
      541 ccgcgccatg ccggaccgca gcgtggccgc tcgcgtcgta gagtgtcgct tgcggactct
      601 tgaacaccca tcccagacgc tgctgcgcgt cgaattcaca cgaatagttc tgtacgccgg
      661 acgcggcgag tgtcatcacg cgttcggctt gcggcggatc gagggatacg gtcggtggac
      721 tcgcaggcgg cgttgcacac gcggctagca gtgatgcggt aacgattgcg aagcttgctt
      781 gcgaaatatt cctgagcatg gatatcacct tgaatgaagc accgcgcatg gcggttgcag
      841 gggaaaacca ctataacgca atgaccgtcc tcggtattga atgcaaccaa accgagtgga
      901 cgcaaagcca atgagcgatc aacagtcata cgcctgtgtg tgctgcagtg actcccaaag
      961 cgctgccttc agccgtcgcc gcttcctgac cctggcggcc gaaagtgccg cggcgctggc
     1021 cttgttcccc gggcttgcct acgcggagtc ggtcccgagc atcgcttacg agtcggtacc
     1081 cgattttctg cgtctcccac gtgacgtcta tttcggcgag tgttcgggcg tgagtttcaa
     1141 ctcgaagggg catatctttg tgctctcgcg cggcaactcg attggccctg cctacggcgc
     1201 ggccgcggcc caacttctcg agttcgcgcc agacggtcgt ttcgtcggcg aaattggcca
     1261 caatctgtat gcctggtcgt tcgcacacac ggtcaaggtc gaccggcacg acaatatctg
     1321 ggtgaccgat aaaggctccg acatggtgat caagttcacc cccgagggac gcgtcgccat
     1381 ggtctttggc cgtaagcagg aggcgtcgga cgaggaaacg gcgccgctca agcatccgaa
     1441 tccgcctttg ccatcggagc ccggacgctt caggcaggtc accgacgtcg cgtgggacag
     1501 cgccggcaac acctatatca gcgacggata catcaattct cgcgtggcga aagtcgacaa
     1561 ggacggcaac tggctcaaat cgtggggcga tcgcggcaca ggtccggggc agttccatac
     1621 gccccatagc atcgccgtcg atgcgaaagg cctcgtctac gttgccgatc gcagcaaccg
     1681 ccgcattcag gtgttcgacg gcgaaggcaa tttccagcgg cagttcacca tcgacgtacc
     1741 ggtgccgccc ggcgctcaac ccgcaatcgg caatgtgccg gacgaagcca cgatagccgc
     1801 gggcaccttc gcgccaggct cgccgtgggc gatctgcatt tcgccgggcc cgaatcaggt
     1861 gctttacagt tccgacgcgt ttccgggccg catctacaaa ctgacactcg aaggaaaact
     1921 gctcggcgtg ctgggtcaat ccggcaagca actgaagcag tttggctgga ttcatcaaat
     1981 ggcctgtccg tccgagaatg tgctgtatgt cgctgagctt ttgaactggc gagtgcagaa
     2041 gctcatgctg cgtccctcgt agcctgctga cgccgcgctc ggcggctcgc cgctactgct
     2101 cccccgcgac gcggtaaatt cggttccgca ctaccgcaat cgcctgcttg agtgcgtaaa
     2161 catcctggaa gtaacgttgc ggcatccgga tccggttcgc gtgtgcatcg atgtgctcga
     2221 tctcgtcctt ccactgcgcg atctggtccg cgtctggatg gctgtttttc catatctcat
     2281 cttcgagcgc cttgatctcg cgataccaga caaccaggcg cttttttatg cgcgtctcta
     2341 ccatgcgcgg caacgcctgt aacaggccga gcagtaaagc catgaagggc agcaggatca
     2401 gcagacggcg ttcgataaaa ctggccagcc agaatggcag gtagcgttgc aggaaggggc
     2461 gtcccgtttt gaagtaacgc tcgctctctt cggaaacagg aaattcctcg gttttcagat
     2521 tgggaaacac gccaagcggc gtgaagtaat cttcgccgcc gtggacagtt ttggccgcgt
     2581 ccagcaacag gtagacgagc gcggggtgca gcgtgtcctt ggcgacgagc aaggccgtgg
     2641 cggcgagcag cgtgacatcg ttttgcggca gatcgttggc gatactggtc gcggcgcgcg
     2701 ggaaaatcac cttggatagc gacgggaatt tctgcaccag cgcatcggcc tgcgcaaagc
     2761 tcatcagttt gagattgctg ttgagcaacg ttgtctgcat cggcgcatcg ggcctgccga
     2821 tgaagaaagc cgcatcgagt tgaccgtttt cgagcgcctg ataggccttg gtggcatcca
     2881 tttcgagcag cgtcgtgttg tcccgtgtga cgccgctgta gccgagcaat agctgggaga
     2941 cgttcaggag gccgctgcca ggaacgccga tggatatctt tttgccgcgt agttgtgcca
     3001 gccggttgat ggtggaatcg gccgagccgc cccggtagaa cacccagatc ggttcatagg
     3061 agaccgcggc gatcgtttgc agatggtcgg tttctttggg actagtcgtg ccggactgaa
     3121 tgaagcccac ctcgtatgcg ctgtccggat ccttcaagcg ctgatagttt tcactcgaac
     3181 ccgacgaacc gcggatatcg agcgtgatgc cgtcacgttt caggacgggc gcgtagcgat
     3241 ccgcaaagcc gcgatagatg ccattgtccg caccggttgt gatgacgatc gtgtgccgga
     3301 aggcgggctc gacgatggcc gatatgcccc agccgagcgc gacgatcagc agtattgcgc
     3361 cagcgatcag aagccgccgg cgacccggtg tcatggggcg tttctcattg cgaagaagcg
     3421 cggggttgtg cctgggcatt agcgttgacc ttgccgcgtc gctggtgctg tttaatagtc
     3481 tgcatggata atgcagtgcc tctaacgcct tgtccacatc gccctcgttg cggagcacct
     3541 ggaccatgcc cgccgtgccg tcggcgttgc gtcgctgacc gttctaaaac cgctccaccc
     3601 tgaacggccg cgtgtccacg agcgtctctt cgtgggtcat catctcggcg atcagccggc
     3661 ccgtgactgg gcctagcgtc agcccgtggt gcgcatggcc gaacgcaaac cacaagtctt
     3721 tgtgatgtgt cgcgggcccg ataatcggca tcatgtcggc tgtgcagggg cgccgtccca
     3781 tccatgcttc gtcgtcgaga cgctggccca acggaaacag cgtgcgcgca atcggctcga
     3841 cggcggcgag ttgcgtgggc gttttgggcg catcgcagtg cgcgatttcc gcccccgtgg
     3901 tcaagcggat gccttttgcc atgggcgcca gcaggtagcc gttctcgacg tccagaaccg
     3961 gatgattcag gcgcgccgat gcctctgacg catagtgcat gtggtacccg cgtttgaccg
     4021 caaggggcag ccggtagccg agccgtgaac tgacgcgatc ggaccacggc cccagcgcaa
     4081 caaccgccga tgaagcggcg atcgttccga cttccgtatc gaccgcccaa ccctcacgaa
     4141 acgagtcggc gtcgccgatg aaaaaccgtc cgcctagcgc ttcgaagtat tttgcgtatg
     4201 cggtgatgag cgcgttgggg tcgttcacgg aatcggatgc cgtataacgc aacccgcctg
     4261 gcaaggactt gtcgagatcc ggttccttct gttgcaggcc gcttgcgtcg agcgcttcga
     4321 actcgacgcc atattcccga tgccatcgtt cagcgatccg ggtttccgcg tcccgtaccg
     4381 gttcggtgcg aaacaccttg atccagcccg tcgggcgcaa caaggcgctc gcgccggcag
     4441 ccgcggccag cgcgcggtgc tcgtccacgc aatgctcgat cagcgtcgag tacgacttcg
     4501 cgatcgcggc gtgttttgcc ggatgagaat tgcgccagta ctgccacagg aacggcgccg
     4561 tcttgaggat ggcgtcggca tggtagcgaa cgtccagcga ctggttgcgc gcgtagcgaa
     4621 gcaaggtgcc gaggtcgcgt ggaaacgcat agggatacac gctttcgcgc tggatcagcc
     4681 cggcgttacc gaacgacgtt tcgttgccgg gcagtttgcg gtcgactagc gcaacctggc
     4741 ggccgcgttt ctgaagatgc acggcaacgc acacgccgac aatgcccgcg ccgagcacga
     4801 cggcatcgaa tttcatggtg gtcatgaact cattcttgct tttggattct tgaaatacca
     4861 gcgccaccct gcccggactc gttgagtgta taggcaacca ggcccaggca agtggcaagt
     4921 ggcgagcgga tcgaggctga cgctggcgtg gcaactggct tcgactattc gttcgcggat
     4981 tcgacgggcg cctgggcgtc tcgcctgggc cggcggacgg ctctcacctt cccactggtt
     5041 ccgcccccgc agaagaactg atcgccgcca tcggactcga gccccgacac gtagacgcct
     5101 ggcggcatct cgaggctctc cagaacttct cccgtacggg aatcgacgtg ccgcaactcg
     5161 ctttcgtcat cttcccaggt ggcatgccag agctcgccgt cgttccaggt gacgccggtg
     5221 acgaagcggt tggacgcgat cgtgcgaaga atcgcgccgg tttcgggatc gacctgaaga
     5281 atcttccggt cctgataccg cccgacccag agcgttcctt cagcccacgt gagccccgag
     5341 ttaccgctgc cgccgccggg tgcggggatc gtcgcgagta cgcggccgct atcaggatcg
     5401 atcttctgga tgagatcgcc ggcaatctga aataaatgct ggccgtcaaa ggccgttcct
     5461 gcctgcgcgg cgacatcgat cgagcgcagc gtttcgccgc tcaccggatc gaaggcgttc
     5521 agcttgtcgc cggtggcaat ccagacgtgc tgaccgtcat aagtgacgcc gtgcactttg
     5581 tcgacgcccg gaaagggtcc atattcacgc acgattctgg cggctgatcg gttcatggtt
     5641 ccatcctgat cactcggtta aaggagcggg gagtaacaat gttgtcgcga atccgggcat
     5701 gggcggcgtc atccagcgcc gcgcccgccc gcgaccgaac gattgcacct tgccgcctgc
     5761 cgccagcgcg tcgagggcgc gctgcatggt gcgctggctg gcgtccagcg caatggcgag
     5821 cgccgaactc gaccacgatt cgccgtcggc gaggaaggcg agcaccatcg cgtgcggttc
     5881 ctcaaccggc cgcgcgagca cgacgacctc gcgcgcgccg tgcggtatca gcgcgaagcc
     5941 ccgcttggtc gcgttcacgc cggcgagcgt tccaagcaac gcgcgaagcc gcccgatttc
     6001 gactcgcagc cgcgcgcgat gcgaatcatc ggggtgcttg gtccggaatg cccgcgcgat
     6061 gagtgcgtcc cgcgagacgt cctcgggcca cgcttcggcc agcgcgcggg cgagcgcgaa
     6121 caacaccgga cgcctcgcga gcgggaccac gatgcgtgct tcacgcacga cataacggca
     6181 cgcgtcgacg atcagcgcct tcgacgccag taacgcttca acctcttcga gcagcagaag
     6241 ccgctcctcg ccatgcgcaa tcagacgcgc cgccggcgtg ttcaggagaa ggcgtgcgct
     6301 ttcgacctcc gccgtcagcg atggaatccc cgcatggtat gcggcgcgct cggctctcgc
     6361 gagcgctgag cgcgccgtat ttgtctggag gcggcgcatt gcgagccccg cgacgaccag
     6421 ctcgtgggcg gttctcaagg ggggcgggaa gggcgcgggg tcgagcctgg cgagcaggcg
     6481 cccggcctcg tcgaggcgcc cgatcagcag caggcgccgc acttcgagat accgcgcatg
     6541 cgcggcgttc agccggtcgc cgtgggcttc gagcgtcgcc cgcgcggcgt cgagcgcttt
     6601 cgcgggccat cccagatcgc gtgaggcgag cgcgatttcg gcctcggcga ccacgcacct
     6661 cgcgcgggcc acaggctctt tggggccgaa ggcgcgcgcg gcgcttcgca ccagcgcttt
     6721 cgcccgaacg agatcgccga gctgcgccat cgcgatgcct cgcaaagcga gcgcaggcgc
     6781 gtcatcgcgc agggcgacgc ggttcagtgc gccgagcggg tcgcccgccg cgagcgcccg
     6841 cgccgcggct gtcactagcg agtccatggt cgattcaaat cgcgccacac ttgtcactcc
     6901 catcgtccgg tgcccgtgac taatctagca cgaccaccaa ccagaacgtc gtatcgacaa
     6961 ctgaacgatc gacgcaggac tcccaaggag ggacgcagga tgacaacaca taccaccggg
     7021 acacgtgaag cgtggctggc ggcgcgactc gagttgctca aggcggaaaa ggcgttgacg
     7081 cggcagagcg atgagcttgt gcaacagcgg caggcactcc cctgggtgcg ggtcgacaag
     7141 gcatatcgat ttgaaaccgc tgcggggagc gcctcgttgg cagagctttt caaaggccgc
     7201 tcccaactcc tcgtgtatca cttcatgttc ggccccgatt acaaggcggg gtgtccgtcg
     7261 tgctcctcga ttgcggatgg gttcgacggc atcgtggttc atctggcgaa tcacgacgtc
     7321 acgctcatgg cggtgtcgcg ggcgccgctc gagaagctgc agacgtatca gcgccggatg
     7381 gggtggacgt tcccgtgggc gtcctcggct ggcagcgact tcaacttcga cttcaacatc
     7441 gccttcaccg aggcgcaaca gcgtgaaggg cgcatcgaat acaactacga gcggggcggt
     7501 cacgcgatgg acgagacgca agtcccggcg cccgtcgccc agttcgcggc cacctgtgga
     7561 accgacgcgg cgacgttcac acgcgaccgg ccgggcttga gcgcgttcgt actcgaggac
     7621 ggcgtcgtct atcacacgta ttccacctat gcgcgcggcg tggacggcat ctggggcatg
     7681 taccagtggc tcgaccgcgc gccgaagggg cgcaacgaga cgggtgtctg gtggaagcgg
     7741 cacgacgagt acgagcagcg ctgagcccga tgatggaacg ggtttgcgca accggggcgc
     7801 ggcagatgtc gggacgctcg atcgacgcgc gagtttcgca gcggacgttc ttcggcgttt
     7861 cggcgctgct cttcgtggtc agcactgggg tgacgatcgc tggcgccgca tccatgtccg
     7921 cgatgggcga gatgccgatg cccggcggct ggacgatgtc gatgacgtgg atgcggatgt
     7981 gcgggcagac gtggcccggt gtagcggcgt cgtttctcgg catgtgggac gtgatgatgg
     8041 tggcgatgat gctgccatct ctcgtcccga tgttgtggcg ctatcgcgag gccgtcggcg
     8101 gaatctgcga ggtgcgtctg gattggctga cggcgctggt gggcgtgggg tacttcttcg
     8161 tgtggagcgc gttcggcatg gccgtatttt cgctgggcgc tgcgctggcg gcgctggaga
     8221 tggaactgcc ggcgttggcg cgtgccgttc cgctcgcggc gggtgtgatt gtgctgagcg
     8281 ccggggcgct gcagttcact gcgtggaagg cgcgccgcct tgcctgctgc cgtgcggcgc
     8341 cggggggctg ccttgtggtg cctgtgacgc ttgcgttgcc cgcattgcct gcattgcccg
     8401 cggacgccgc cttggcgtgg cggcacggct tacgcctcgg ccttcactgc tgctactgtt
     8461 gtgccggtct gacgatggtc cttctcgttc ttggggtgat ggatttgcgt gtgatggccg
     8521 tcgtgacggc ggctatcacc gtcgaacgtt tggctccgcg tggagagcgc gtggcgcgcg
     8581 tgattggcgt gggtgtcgtc ggggcggggt tggtgctgat cgcgcgagca gtggggcttg
     8641 gatgacctgg tgaagcatcg aaagcccgtt gccatcgcgc gataaggaag tttatccgtc
     8701 gtcggatcaa cgtatgccca catacagcga acaacggaat ctgacccaca agtagcacag
     8761 gacgctgctg gcaacagcgc gcgcccgtga tgcgaccatg accgtgcggt tgttgcagtc
     8821 acggtcacgt tcagtcgaaa tcgaacacgt cgaagatcga gcgtttcttt ttgtgcccgc
     8881 gataatccgg tcggcgatcg tcgtgcgtgc ggttgttttc atatgcatga tctcgatcgc
     8941 ggtggccgtc ccgggggctc tcccgatgac tctcacgggt tacgcgtgcc gacgcggcag
     9001 catcggcggt cccagcgtcc agcgcgatca acttgtcgag ttcgccacga tcgagccata
     9061 tgccccggca gaccgggcag tagtcgattt cgatcgagcg ccgctcgctc atcagcaagt
     9121 ccgtggtctt gcaaacaggg catttcattt cgttgctcct caagggtaca aaaagacatc
     9181 gaacgctcga cataacgagc tgcccgactc cgaaaaaacg atccgacgtt acttcgttcg
     9241 aggatgtcga gcgcgctttt catgtgcgga cgtcatagcg gcgttcacta ctccgcgtta
     9301 atcggccgct tcgatttcgc ccggcgcacg agcatgttca atgcctcgac aaaggcggag
     9361 aacgccatgg ccgcgtagat gtaagccttc ggcacgtgcg aaccaaatcc ctcggcgata
     9421 agcgtcatgc cgatcacgag caggaagccg agcgccagca tgacgacagt cggattacga
     9481 tcgatgaagc gcgacagcgg gctcgcggcg aacagcatca ccatgaccgc tgcaattacc
     9541 gcgatgaaca tgatcggcag gtgctccgtc atcccgaccg cggtgatgat gctgtcgacc
     9601 gaaaacacga tgtcgagcac caggatctgt ccgatcgcag ccaatgcggt caactgcacg
     9661 gttgtgttcg ccttgtcgcg tgcttcttcc gaatgaacga cgtggtgatg aatctcactc
     9721 gtagccttcc agacgaggaa caggccgccg cccagcagga tcaggtcgcg ccacgaaaac
     9781 gcgtggccga acagcgccac ggcgggtgcg gtcagttgcg cgatccacgc gacggtgccg
     9841 agcaggccga gccgcagccc gagtgcgagc atgatgccca tgcgctgcgt gcgcgcgcgt
     9901 tgtgcttctg gcagcttgtt gctgaggatc gagatgaaga tcaggttgtc gatgccgagc
     9961 acgatctcca tcacgaccaa ggtgagaagc gcagcccatg cggcggggtc ggcaatgagg
    10021 atgagtaggg cgtccatggg gtccgtaagc gaagggtgac gggtaacgtc ggtatgccgt
    10081 catcatcgcc gtcctgatgg tgcggataaa tcagtgataa tctgagcgat atgtcggttt
    10141 tttcgaagtg tccatcctat gctcaactac cgccatctct actatttctg gatcgtcgtg
    10201 aaggagggcg gctttgcgcg cgcggccgag cgtctcgaca tggccgttca gacgatcagc
    10261 gcgcaggtgc gcgaactgga aaaatcgatt gggcgtcagt tgctgaagcc ggcagggcgc
    10321 ggcgtcacga tgaccgaagc cggcgagacg gcattcaatc gcgcggagca gatcttccag
    10381 atcggcgagg cactgctcga cgagatgcgc gaggcgggcg gcgaacgtgt tgcgcggctc
    10441 gccgtaggtc tttccgacgg catttcaaag ctcgccgcgc acgcgctgct ggcgcccgtg
    10501 ctcggcacgc cgtcgctcag gctgctatgt catgagggcg aacctgcgca gctgctgtcg
    10561 gaactcgcgc tccatcggct cgatctcgtc cttgcatgcc agcccgcccc gcataacgcg
    10621 gacctgcgcg tggtgagcca gcggctcgtg gggtcgcccg tcgattggta tggccctgcg
    10681 gagatcgtcc gcaaatcggc ccgtgccggc tttccgcaat gcctggccga tctgccggta
    10741 cttctgccca ccgggcatgg cgcattgcga gcgcggctcg accggtggtt cgaagccgca
    10801 gggattcgcc cacggatcgt cggggagttc gaagacagcg cgctgatggc ggtgttcgcc
    10861 gcacggggtc tcggcgtgtt tccgctcgcg gaactgggcg cagaagacgt ggcgctgctg
    10921 cgcagactgc gctggctcgg gcgggcggaa ggcgtggtcg acgagatcca cgccatccgc
    10981 tcgcggcgcg ggcaacatca tgcactcgca tctcaggtgg ttgccggcgc acgttcctga
    11041 ccgcgcggcg gggggcctta ccggcttgct gcccgtgcag gtgcagaccc ccacgcgttg
    11101 aacacggcgg cgagcaaggc gatttcgccg tccgccagat agtcgtccgc gatcgtgacg
    11161 gcgatgcaca ggcgaatcac cttgcggcgc aacgcggggt cgtcgatctc gtcgatcaag
    11221 gtggcgagga gggccgcgtc gaggtggccg accggcggtg cgaatggcgt gtgcgcgatc
    11281 gactgatcct cgcacagcgc ctggacgatc cgagcgaatt gcgccggcgc gaggccgagt
    11341 tcgtccgcga tatccaggcc ttcgagtgcg cgctcttccg cgctgctgac gtggccgtcg
    11401 gaagtgagcg ccagcgcgac gatgcgtgcg gcggcttgag gactgttgcg cggataggtt
    11461 cgcatgttgg ggctccttgc gcggacgctg tccgcagtga tggtgagcct cagtttgcgt
    11521 tggggtgggg atcgggtaaa tctgttaatt cgggagtgaa ggttcggaaa atccgaagtg
    11581 tatacgcgcc agcccgcacg aagcctcgtc gttctgacgc aatgcaaaaa cgccccatcg
    11641 gataagatac agctcgccgc aattttccaa atagaaggag tgttttcagt gattcaaccg
    11701 agcaacgtgt tcaaggacaa cctcgcccaa ctgcctgcca ttgacggtat cgaacgcatc
    11761 gatctggtcg acggcaaagg cgccgtggtg gcaagcattg agaacaagcc gggcaagcag
    11821 ggttcgctgg ccgtgtacca ctatctaaag caggcattcg gcacgctgga cgccaaggca
    11881 gccgaacacg gtctgctggt gttcgccgag catacggccg acgcccgcaa tcgcccgggt
    11941 gcgcacccga acgtcgaccg tttgctggcg atcgtggccg gtggggaagc gttgcgcatc
    12001 gacgtcgtcg cagcgggcta agcgcgaata aggtcaacga acgccctgag cagagccggc
    12061 atatgagtcc ggctcgggtc gtacaaaacc gatcgcacgc ggaaattcgg cccatacttg
    12121 cggtcaagcg ccctgcttgc ggcgcaacta gcgaggaaca tgatgaagca acttcaactg
    12181 ggtaagacgg gtccgcaaag cgccgccatc ggcctcggct gcatgggcat gtcgggcatg
    12241 tacggtcctt ccgaccgcgc tgaaagcatc gccacgatcc acgccgcgct cgaagccggc
    12301 atcacgctgc tcgatacagg cgatttctac ggctcgggcc acaacgaaat tctgatcggc
    12361 gaggccctga aaagcgcgcc gccgtcacgc cgcgacgcgg cgatcatcag cgtgaaattc
    12421 ggggccatgc gagatcccgc cggtggctgg tcgctttacg atgcacgtcc cgcggcggtc
    12481 aaaaattttc tcgcttactc gctgcagcgc ctcggcgtcg aacatatcga catctaccgt
    12541 cctgcgcggc tcgatcccaa tgtgccgatc gaggacacgg tcggcgcgat cgccgacatg
    12601 atcaaggccg gttatgtgcg gcacgtcggt ttgtccgagg taggggcgac gaccctccgc
    12661 cgcgcggcag cggtgcaccc ggtgagcgac ctgcagatcg agtactcgct gatttcgcgc
    12721 ggcattgaag acgagatact gccggcgtgc cgtgagctcg gcattggcgt cacggcgtat
    12781 ggcgtgttat cccgcggcct gatcagcggc cactggcgca aggacgccgc gggcaaaggc
    12841 gattttcgcg ctatgagtcc gcgcttccag ggggacaaca tcgatcacaa tctcgcgctc
    12901 gtggaagcgc tgcgcaaggt ggctgatgca aaaggcgtct cggtcgcgca gatcgcgatt
    12961 gcatgggttg ccgcccaggg cagcgatatc gtgccgctcg tcggcgcgcg ccggcgcgat
    13021 cgtcttgccg aagcgctcgg tgcggttgac gtgacgctca cggcggacga tctggctgag
    13081 atcgcgcggg ctgtgccgat cggggctgcc gcgggcgatc gttacgcggc ggcgcagatg
    13141 gcgcatctcg acagtgagaa gggcagtcac ggacacgctg cctgagtgcg tcgacgggtc
    13201 atgcaaaccg ggtgagccac gcattgttga atgtgggtag cgtggcttcg gtaccgaatt
    13261 ttcaaccggc gggcgcccgt agtacttcaa ccggctgcta accggctgtt cgcttaagga
    13321 accggcggga agtgccgctt gccggtgtgg acccgcaagc gccgaaaacg ctctttctta
    13381 tgctcctcat cgaagtgggc aagagacggt ttgaccagat cgctgcgcga cacaatgccg
    13441 accaactgca acgcttcggc gtccgcgacc acgggcagcc gttcgagacc gtgcaccgcc
    13501 agccgcgttg caacgagccg gcaggtttca ctgggcaatg caaaagcggg ggagcggtgg
    13561 cgcaatacgt cggcgaggcg cgagtcgagc gcatgcccgg tctcgtcatc gcgaactctt
    13621 tccagcaccg cgcgatcgac gacgcctgcc agcacgccct gacgcacgac cggataggcg
    13681 cgatgtgtct gcgtcgtacc gaagtacgtt tccagcgctt cgcccacgga tagatcgcca
    13741 tcgatcgtct cgacggtacg cgacatcacc tcgtcgacgt ggtggcgctc aagcggatcg
    13801 acgccatatt cccggtaaat gtgataaccg cgccgggcaa ttttctcggt catgatggag
    13861 cgcctcatga cgaccgttgc aaacccgtgc gcgaccagcg tcgcggcgag cagcggcagc
    13921 aaggcgttac tgtcgtgcgt gaggccgaag gcaaacacga tggcggtcag cggcgcgccc
    13981 agcgtggcgc cgagcgtggc cgccatgcag acgagcggcc acagggcagg ctcgccgccc
    14041 ggcagcacgt ggccgaacac gacgccgagg cccgcaccca gcatgagcaa cggagcaagc
    14101 acgccgcccg aggtgcccga gcccagcgcc acgacccaga tgatcgcctt tacgaccagg
    14161 atcgccagcg cgatctggat tgcaatgtgc tggtgcagca gatcgccgat cacgtcgtag
    14221 ccgacgccta gcgcgcgagg ttcgaaaaag ccgccgatgc cgacgacgag gccgccgagc
    14281 gcgggccacc acatccagtg gatgggcagc ttgccgaaca ggtcttcggt cttgtagagc
    14341 gcggccgaca gacccgatgc gagcgcgccg gacatcaacc ccgcgatcac gcaggacacc
    14401 agcgacatgg cgccaggcgg cgcggtctcc agcggaaaca gcggccccgt gccgaaaaat
    14461 gccgcgcgcg caaagcccgc gaccgcgcat gcaagggcga ccggcagaaa actgcgcgga
    14521 cgccattcga acagcagcag ttcgaccgcg agcaagacgg ccgccaccgg cgtgccgaag
    14581 acagcagtca tgcccgccgc cgcgccggca accagcagcg tcttgcgttc cgcggaggtg
    14641 accttcacgc attgtgcgat caatgacccc agtgcgccgc ccgtcatgat gatcgggcct
    14701 tccgcgccga acggcccgcc gctgccgatc acaatgccgg acgagagcgg cttgagaatc
    14761 gccactttcg gcgacatgcg gctcttgccg aacaggatgg cttcgatcgc ttcaggaatg
    14821 ccgtgcccac ggatcttctc cgagccataa cgggccatga aaccgacaat gagcccgccg
    14881 atcacaggga tgatcaccac ccagataccc agcgtgttga cggccggcga acggtccgca
    14941 aacgagaatt gctggaagat aaacagattg gtgaacagat gaatcaggct cagcagtacg
    15001 aacgccgcga gcgtgctgag gacgccgatc cccgcagcga gtgcggaaat gccggggagc
    15061 cgcgcgttgg tggaaaagtc gcgcttatgg gtatcgagac tcatggtgac tcaataatcg
    15121 atctgaggga cgttaaaggc gcctttgagc gacttcagtt cggctcggtg catttcggcg
    15181 agacgggcaa gcacctgctc gcctgctttc aacaaatgaa cctcgacctg gcggcggtcg
    15241 gtctcgctga cctgcctgcg gaccagatcg agcgcctcac agcgggatac gagcgcgacc
    15301 acgccgtgat gctgcgcctg gagccgctcc gccagctcgc cgacggttgc ccagtcgcgc
    15361 tccggataac ctttgatgtg tagaagcagc agatactgca acggcgtaat gccttcgccc
    15421 tgggcggcct gctcggaaaa acgctcgaag cgccgcatct ggtagcgaaa ctcggacagt
    15481 tgctcgaagt ccgccttggt caggttgcga gcgagggttt tcatcggcgt tccgtgcaga
    15541 aatagctgaa acgcgaatta tatcatgacg tgatatttat gcgcgtgttc gaaccgcggc
    15601 atgcatgacg cgggttatgc cgcgcgttgt tgttgctgag cgtaagcctg gcgatacaga
    15661 ccggcgatga ggtcggcctg cgtgatcatt ccagccagcc gccgcttcga gtccagaacc
    15721 gggatgtggt ggtgcccgta gttcgcgaac attggcacca actcgacgat cggcgtggtc
    15781 gcgtcgacgg tatgaacgtc gacgttcatc agagcgccga ccagcggcgc cggcgtgata
    15841 ctgcgccgca cccagcgttg caggggagac gacagaccgg cccctctgct gaacgcgcgt
    15901 ttgtcgacca ggtcggcgcg ggtcacgatg ccgataacgt gctgcttctc gtcagtgacc
    15961 gggagtgcct tgaggtgccg ctgtttcagc agactccatg cggcggaggc tctggtgacg
    16021 gccgacacgg cgacgaggga gcgcgacatg atgtccgcgc aggtcagttc gttgaacgtg
    16081 cgggcgtagg cctgcaactg aacatcgcgc agcagggatt ccagatcgtc cgtgccgata
    16141 tcgagcaatt cgccgcgccg gttcagcacc gcctccaggt ccgagcgcgt gaaaccttcg
    16201 ttcgacaccg ccgccgccgt gcctttttcc gagttctgcg cgacgtgcgg gtatcggtgc
    16261 cccgtagcag cgtgatagat gacggccgcg cacaacagca tcagtgactg gatcgcaatc
    16321 ggttcggcca caaagcggta gcccagagcg tgcactgccg gaccgccaag gacggcggtc
    16381 agggccaccg cgcccgacgg cgggtgaacg caccgcaacg caaacatcgc gcagatcgcc
    16441 agcgcgacgg ccaaagcggc ggcgcccacc ggatcggcga tccagcttgc acaggcaacg
    16501 ccgaccgtcg cggagacaat attgccgccg atgatggacc acggttgcgc aagcggactg
    16561 gcaggaaccg cgaacagcag cacggccgaa gcacccattg gcgccacgag aagcggaatg
    16621 ttcgccgccg gtccgagaag gacgtgcatg gtcccgcccg tgaacgcgat ccccagcaat
    16681 gcaccgagac acgatctgaa gcgttccggc caccggacgg caatgggatg aggggcaaag
    16741 ctggaaaacc agcggatgag ggcgtctcgg gacaagattg gaataggaaa gtgaggggcg
    16801 gtatcgcatg gaacgaggcc acaccttacg gccaaatcct cacgaagcga aagaacgcat
    16861 cgtcataatt atatttcatt gtgatataaa atgcgggcgc cgcttctcgt gacggccacg
    16921 gcctcaccgt gcgaaatggc gcgctcacgc gcgcgcagac gcttccagtt cagcaacacg
    16981 cttgcgcagc aagcgatcct cctgaaagcg gctggtttcg tcgcggatca cggcgacgat
    17041 gcctgtgagt tcgttttgcg gcgaatgcag caacgcaacc gtaaaggcaa tcgataaggc
    17101 ccgcccgtcc ttatgcaagg cgggaacacg cagcacgtcg ttgccatagc tggtttcgcc
    17161 cgttgccatg gtcttgtgat agccgtccca atggcggccg cgcaaacgtt caggaatgat
    17221 cagatccagt gacttcccca gtgcctcgct ttgcgtgaag ccgaacatgc gttcagcggc
    17281 aggattccac aacgtaatgc tcccgccggc atcggacatg atgatcgcat cgccgattgc
    17341 gttgacgagt tgctcgaagt cgatggctgt ttgcatggca ttccctgaat gtaaaacgca
    17401 aatccttgaa tgggatacgg cgcccgttga agggcgccgt atccgcaggc ccaggcatac
    17461 caacgggcct gcggtgtacc accattaaac cgctttgatt tcgtgcaggt tgaagatgtc
    17521 ctgccggctg tagccgaggc tcgccagcac ttcatccgtg tgttcaccga gcagcggcga
    17581 cgcggtgact tctggcttca tatccgagaa tttgatcgga ctgccgactg tcagatacga
    17641 gccgcgtttc ttg
//
LOCUS       DATASH010000535         3054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3845211,
            whole genome shotgun sequence.
ACCESSION   DATASH010000535 DATASH010000000
VERSION     DATASH010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3845211"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..104
                     /locus_tag="VHV99_14895"
     CDS             <1..104
                     /locus_tag="VHV99_14895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010116266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX3636743.1"
                     /translation="YDLLEGFEGDKDGQGHRKTVSGWCFRGLPWIGA"
     gene            complement(193..1029)
                     /locus_tag="VHV99_14900"
     CDS             complement(193..1029)
                     /locus_tag="VHV99_14900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEX3636744.1"
                     /translation="MAFGVIIIGDEILSGRRIDKHLPKVIELLGARGLSLGWAEYIGD
                     DPERITATLRRTFASGDIVFSTGGIGATPDDHTRQCAAAALGVPLELHPEAAKLIQER
                     IRDMHPANSAVPLDLETPENRHRLNMGTYPQGAAIIPNGYNKIPGFSIKQHYFVPGFP
                     VMAWPMIEWVLDTHYAHLHHAMPHAEKSLLVFELPESRLTPLMEKIEHDFPGVRVFSL
                     PSVGDAERGGVYARHHIDLGVKGEPEAVAAAFVKLREGVHLLGGDIVEPEVAAAAAKP
                     RG"
     gene            complement(1060..1890)
                     /locus_tag="VHV99_14905"
     CDS             complement(1060..1890)
                     /locus_tag="VHV99_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HEX3636745.1"
                     /translation="MNDLLRSFGRALTSALHPRMLWLTFLPFLAATLGWGVILWFSWQ
                     TLIGATRTWLESWSFTVTLYHLFDWLGFSALHVAIAPFIVIAMAIPLIVVTVLLLIAT
                     LSMPAVIRFLSARQFSGLEMRRGGTWYGSLGQALWTTLICLVLLIVTVPLWLVPPFFA
                     LIPPLLWGWLTYRVMSYDALALHATRDERRALVRRFRLPLLLIGIASGLLGSLPTLLW
                     ASSVWMIVLFPVITAVTIWIYAFILVFSALWFGYYCLRALQRMRAEEHGPRHAAPVPY
                     "
     gene            complement(2113..>3054)
                     /locus_tag="VHV99_14910"
     CDS             complement(2113..>3054)
                     /locus_tag="VHV99_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX3636746.1"
                     /translation="LYVDVVQPLLFRFYLMGYDEDTYDSLYWVIVGVFEVLAMYAILR
                     PLEALRPVEQWENRKAVRVDVIYTWIVKLGILNLFFFFALQPFFDNVQAWLRLHNIAN
                     LEFDNLWPGVTTQPLVTFVMYLLVLDFAGYWYHRWEHRIGVWWELHAVHHSQQQMSLW
                     SDDRNHLLDDLLQASFFAVIALVIGVPPSQFVVLVAITNLAQSVQHANIRLYFGWLGE
                     RLLVSPTFHRRHHAIGYAHEGLKYGCNFGVLFPWWDMLFRSVSWSREMEPTGIQDQLE
                     GRRYGDGFWAQHWLAFVRIAGRLSPKRRAEGSDAAPI"
BASE COUNT          675 a          970 c          933 g          476 t
ORIGIN      
        1 cgtacgacct gctggaaggc ttcgaaggcg acaaggacgg ccaggggcat cggaagacgg
       61 tgtccggctg gtgctttcgc gggttgccgt ggatcggcgc ctgattgccg atcagttgac
      121 ttgatcgtcg cggcccgctt cagccgtaac tgcgggcgcg tattaccacg cccgcctcga
      181 agcaagcgcg gatcaaccgc gcggttttgc cgcagccgcg gccacctcgg gttcgacgat
      241 atcgccgccc agcagatgca caccttcgcg cagcttcacg aaggccgccg ccaccgcttc
      301 cggttcgccc ttcacaccga gatcgatgtg atgccgcgca tatacgccgc cgcgctccgc
      361 atcgcccacg ctcggcaggc tgaacacccg cacgccggga aagtcgtgtt cgatcttttc
      421 catcaacggc gtaagacgcg actccggcag ttcgaacacc agcagtgact tttccgcgtg
      481 cggcatcgcg tgatgcagat gcgcatagtg cgtatccagc acccattcga tcatcggcca
      541 cgccatcacc gggaagcccg gcacgaagta atgctgtttg atcgagaagc ccggaatctt
      601 gttgtagccg ttgggaataa tggccgcgcc ctgcggatac gtgcccatat tcagacgatg
      661 ccggttctca ggcgtttcga gatcgagcgg cacagccgag ttcgcgggat gcatatcgcg
      721 gatgcgctcc tgaatcagct tcgcggcctc gggatgcagc tcgagcggca cgccgagcgc
      781 ggctgccgcg cactggcggg tgtgatcatc cggcgtggcg ccgatgccgc ccgtggaaaa
      841 cacgatgtcg cccgaggcga acgtgcgtcg cagcgttgcc gtgatgcgct ccggatcgtc
      901 gccgatatat tccgcccagc cgagcgacaa cccgcgcgcg cccagcaatt cgatgacctt
      961 cggcagatgc ttgtcgatgc gtctgcccga caggatttca tcgccgatga tgatgacgcc
     1021 aaatgccatt gctgcctctt tcgcttaatc agtaattcat cagtaaggaa ccggcgccgc
     1081 gtgccgcggg ccgtgctctt ccgcacgcat gcgttgcagc gcgcgcaaac agtagtagcc
     1141 gaaccacaac gccgaaaaca cgaggatgaa cgcgtagatc cagatggtca ccgcggtgat
     1201 cactgggaac agcacgatca tccataccga cgacgcccac aacagcgtag gcagtgagcc
     1261 cagcagtccg cttgcaatgc cgatcaacag gagcggcagc cggaagcgcc gcaccagcgc
     1321 gcgccgctcg tcacgtgtgg catgcagcgc gagcgcgtcg taactcatca cccgatacgt
     1381 caaccagccc cacaggagcg gcggaatcag cgcgaagaac ggtggcacca gccagagcgg
     1441 caccgtgacg atcagcagca cgagacagat cagcgtggtc cacaacgcct gaccgaggct
     1501 accgtaccac gttccgccgc ggcgcatttc gaggccagaa aattgccggg ccgacagaaa
     1561 gcggatcacg gctggcatcg acagcgtggc gatcaacagc aagaccgtga cgacgatcaa
     1621 cggaatcgcc atcgcaatca cgataaatgg tgcgatcgcc acgtgtagcg ccgaaaaacc
     1681 gagccagtcg aacagatgat aaagcgtgac cgtgaacgac cagctttcga gccaggtgcg
     1741 agtcgcgccg atcagcgtct gccaggaaaa ccacaggatc acgccccacc ccagcgttgc
     1801 cgccagaaac ggcaggaagg tcagccagag catgcgcggg tggagcgcgc ttgtgagcgc
     1861 gcgtccaaac gagcgcaaca gatcattcat gcgagccagg gacggtgaac gaaacgatgg
     1921 aacaagagca gcgcgacgaa ccggcaacgc gtccggcaac cggaagcccg gcgcccggat
     1981 gcccggcgcc cggatctgaa cgccgaccgc aatgcgggcc ggaaaatgaa cctggaacgc
     2041 gcgcgcaaac gtggacagga taaaccacgt gacgcgccgc cgggcaaacc ggcggcgtgc
     2101 ggggaagaat attcaaatcg gggcggcgtc gctgccctcg gcgcgacgct tcggcgaaag
     2161 acgcccggcg atgcgcacaa aagcgagcca gtgctgtgcc cagaaaccgt cgccatagcg
     2221 gcggccttcg agttgatcct ggatgccggt cggctccatt tcacggctcc aggacacgct
     2281 gcggaacagc atgtcccacc agggaaacag cacgccgaag ttgcagccgt atttcagccc
     2341 ttcgtgggcg tagccgatgg catggtgacg gcgatggaag gtcggactga ccagcaggcg
     2401 ctcgccgagc cagccgaaat acagccgaat attcgcgtgc tgcacgctct gcgccaggtt
     2461 cgtgattgcc accagtacga cgaattgtga cggcggcacg ccgatcacca gcgcgatcac
     2521 cgcgaagaac gacgcttgca acagatcgtc gagcagatgg ttacggtcgt cggaccacag
     2581 cgacatctgc tgctggctgt gatgcaccgc gtgcaattcc caccacacgc cgattcgatg
     2641 ttcccagcgg tggtaccagt aaccggcaaa atcgagcacc agcagataca tcacgaacgt
     2701 gacgagcggc tgcgtggtga cgcccggcca cagattgtcg aattcgagat tcgcgatgtt
     2761 gtgcagccgc agccacgcct gcacgttgtc gaagaacggc tgtagcgcga aaaagaagaa
     2821 caggttgaga atgccgagct tgacgatcca cgtgtaaatc acgtcgacgc gcactgcctt
     2881 gcggttttcc cactgttcca ccgggcggag cgcctcgagc gggcgcagga ttgcgtacat
     2941 tgccagcacc tcaaagacgc cgacgatcac ccaatacagg ctgtcgtagg tatcttcgtc
     3001 gtagcccatc agatagaagc ggaacagcag cggctgcacc acgtcgacgt acaa
//
LOCUS       DATASH010000536         2892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6415565,
            whole genome shotgun sequence.
ACCESSION   DATASH010000536 DATASH010000000
VERSION     DATASH010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6415565"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1449)
                     /locus_tag="VHV99_14915"
     CDS             complement(<1..1449)
                     /locus_tag="VHV99_14915"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HEX3636747.1"
                     /translation="MDNKVRQDIEETAETITAPGVSAECRRIVQVILAGGSGTRLWPV
                     SREQFPKQLIGVIGTESLLQATVLRMKHFPAAWDVAAEPVIVCGEEHRFATGEQVRES
                     GVTARIVVEPARRDTAPALTLAALTACADGQDAILVAMPADHAIADLDALHASIETAA
                     RFAQAGDIATLGIPPTRPDTGFGYIRLGDALEDGAHRIDRFVEKPAAELATQYVAAGN
                     YWWNSGIFVVRASVWLAVLEQLQPEMHAACVLSYEQLNNADGYLRPCAAEFSKSPSDS
                     IDYAVMEHLGKPGSPFKGVVVPLEAGWSDLGSWDAVWDAMEKDESGNAACGRVLFEGS
                     TASYARSEGGRLVACVGTTNLIVVETDDAVLVADRSRVQDIKGLVSRIKLAKSPEADA
                     HRKVRRPWGFYDSIDHGERFQVKRIVVNPGARLSLQMHHHRAEHWVVVSGTALVTRGE
                     EEFLLSENESTYIPLGVLHRLENPGRVPLEIIE"
     gene            complement(1594..2595)
                     /locus_tag="VHV99_14920"
     CDS             complement(1594..2595)
                     /locus_tag="VHV99_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3636748.1"
                     /translation="MPYASLEPAVMGWLQPAPRSTQAEIRRIAILLFDGFSLLGAGIV
                     AEVFHVANELAVLRARNDWTYDVRFLSVEGGNVACSSSVRVWTDGLDARHYGGFDALF
                     VAGGKGADGAAGDERIVEWLRRVHSKTTVVKSIAEGRKVLDAAGIGHAAETQIRVGHS
                     MMHAVREEQASEAGDRYESMKGALLMIKRDLGVEVARGVAERLMPGSASKLVPLLGDS
                     GTASAGDKIRTAARWLQDNCERPISVVDAAQVAAMSERNFLRRFKIEMGITPSDYLLQ
                     ARLAVTCTLLTDSELPVDKIARHSGMGNGDRLAKIFRKRLLISPTEYRMQSRRDAGV"
BASE COUNT          552 a          938 c          852 g          550 t
ORIGIN      
        1 ttcaatgatc tccagcggaa cgcggccggg attttccaaa cgatgcaaca cgccgagcgg
       61 gatgtaggtc gattcgtttt cgctcagcag aaattcttct tccccgcgcg taacgagtgc
      121 tgtgccactc acaacaaccc agtgctccgc acggtggtga tgcatttgca gcgacaggcg
      181 cgcgcccgga ttcaccacga tgcgcttcac ctggaaccgc tcgccgtgat cgatggagtc
      241 gtaaaaaccc caaggacggc gaacctttct atgcgcatcg gcttccggcg acttcgcaag
      301 cttgatgcgc gacacaagtc ccttgatgtc ctgcacgcgt gagcggtcgg ccaccagcac
      361 ggcgtcgtcg gtctcgacca cgatcaggtt cgtcgtgccc acacaggcca cgagcctgcc
      421 gccttccgag cgcgcgtaac tcgcggtcga gccttcgaac aggacccgcc cgcatgcagc
      481 gttgccggac tcgtccttct ccatcgcgtc ccagaccgcg tcccatgaac cgagatcgga
      541 ccagccggct tcgagcggca cgaccacgcc cttgaacggt gaaccgggct tgccgagatg
      601 ctccatcacg gcgtagtcga tcgaatcaga cggtgacttc gaaaactccg cggcgcatgg
      661 acgcaagtat ccgtctgcgt tattcaattg ttcgtacgac aagacacatg ccgcgtgcat
      721 ctccggctgg agctgttcga gtacggcaag ccataccgat gcacgcacca cgaagatgcc
      781 gctgttccac cagtaattac ccgctgccac gtattgcgtg gccagctccg ccgccggctt
      841 ttcaacgaag cggtcgatgc ggtgcgcacc gtcctcgagt gcgtcgccca ggcggatata
      901 gccgaagccg gtgtcggggc gcgtaggagg aatgccgagc gtggcgatgt cgccggcctg
      961 cgcaaaacgc gcggcagttt cgatggacgc atgcagcgca tcgagatcgg caatggcatg
     1021 gtcggccggc atggccacga ggatggcgtc ctggccgtcg gcacaggcgg tcagcgcagc
     1081 aagcgtgagg gcgggggcgg tatcgcggcg ggcaggctcg accacgatgc gcgcggtcac
     1141 accgctttca cgcacttgct cgccggttgc gaagcgatgc tcctcgccgc acacgatcac
     1201 aggctccgcg gccacgtccc acgcggccgg aaagtgcttc atgcgcaaca cggtggcctg
     1261 caacaatgac tcggtgccaa tcacgccaat cagctgcttc ggaaactgtt cccgcgatac
     1321 cggccacagg cgcgtgcccg atccgcccgc gagaatcacc tgcacgatac gacggcactc
     1381 cgcgctcact cctggagcgg tgatcgtctc agcggtttcc tcgatgtcct gcctgacctt
     1441 gttgtccatt ttagtattcc tatattctct aatccgttcc acggccggag ctacgcgcac
     1501 catgtgcacc gatcgtcgac cccggttcag ccgaaccgct tacgtctcaa cgctgcgtga
     1561 ctacggtcaa aaaaagcctg gacttaccct caatcagacg ccggcgtcgc ggcggctttg
     1621 catccggtat tcggttggtg aaataagaag gcgcttgcgg aagatcttcg cgaggcggtc
     1681 gccgttaccc atgccgctgt ggcgcgcgat cttgtcgacc ggcagttccg aatcagtaag
     1741 caacgtgcag gtcacggcaa ggcgtgcttg cagcagatag tccgagggcg tgatgcccat
     1801 ctcgatcttg aagcggcgca ggaagttgcg ttcgctcatg gccgccacct gggccgcatc
     1861 cacgaccgag atcggccgtt cgcagttgtc ctgcagccag cgcgcggcgg tacggatctt
     1921 gtcgccggca gaggccgtgc cgctatcgcc gagcaacggc acgagcttcg acgccgagcc
     1981 gggcatcaga cgttcggcaa ccccgcgcgc gacttccaca ccgaggtcgc gcttgatcat
     2041 cagcaacgcg cctttcatgg actcgtagcg gtcgcccgct tcgctcgcct gctcttcgcg
     2101 cacggcgtgc atcatcgaat gacccacgcg gatctgcgtc tcggccgcgt ggccgatgcc
     2161 ggcagcgtcg agcaccttgc gcccttcagc gatcgacttg accaccgtgg tcttggagtg
     2221 cacgcggcgc agccattcca cgatacgttc gtcgcctgct gcaccgtccg cgcccttgcc
     2281 gccggcaacg aacaatgcgt cgaagccgcc gtagtggcgt gcatcgaggc cgtcggtcca
     2341 cacccgtacc gacgacgaac acgccacgtt gccgccttcg acggacagga aacgcacgtc
     2401 gtaggtccag tcgttgcggg ccctcagtac ggcgagttca ttcgcgacat ggaagacctc
     2461 cgcgacaatc ccggcgccga gcagcgagaa gccgtcgaat agcaggattg cgatgcggcg
     2521 gatctccgcc tgcgtgctgc ggggggccgg ctgtagccat cccattacgg cgggttcaag
     2581 actggcgtac ggcatggcat tcccctgcga acttgttgaa tccaacatga ttggatagtg
     2641 ggttctcatt gcggtgcatc atagacagcc tcaagttgtt taggatgtcg tgactgaaag
     2701 tagcgagatg ttggcggaaa aattccgacg tcgccattca aatcctttca gtgcgtgcgc
     2761 tgcctatcac tcggtcaatt tcaatacgca cgttcggtaa atcttgtata gaaccgaatg
     2821 accacgcgtt ttattcccaa tggcacattg caagacgaat gccagtccaa ttcgaccgca
     2881 catatcggac ag
//
LOCUS       DATASH010000537         1955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5033232,
            whole genome shotgun sequence.
ACCESSION   DATASH010000537 DATASH010000000
VERSION     DATASH010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5033232"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VHV99_14925"
     CDS             <1..297
                     /locus_tag="VHV99_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3636749.1"
                     /translation="GHARIIVRYILPNVLPPIIVQASLTIATAIIAEASLSFLGLGQL
                     PPAPSWGSMLNTAKDFVSQAPWMSIFPGIAIFLAVLGFNLLGDGLRDALDPRES"
     gene            360..>1955
                     /gene="ggt"
                     /locus_tag="VHV99_14930"
     CDS             360..>1955
                     /gene="ggt"
                     /locus_tag="VHV99_14930"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX3636750.1"
                     /translation="MTRFNWQNPYPTPRLPVFARNIVSTSHPLAAQAGLRMLWKGGNA
                     IDAAIAAAAAITVVEPVSNGLGSDAFALVWDGKKLHGLNASGVSPAAWNVDYFKRKYG
                     EENGLANHPKRGWDAVTVPGVIAGWEALHQKFGSLPFADLMEPAIEIAERGHAVASVV
                     AYKWAAAVPELKNQPGFADTFMPRGRAPEVSELVRFPGHAKTLRRLAELGPRAYYEGE
                     IAEQIAAFSRQGGGALTMDDLRNYRVDWVEPISKDYRGHTVHEIPPNGQGIAALIALG
                     ILEQFDVGSLPVDGIESQHLQIEAMKLAFADVYQYVADPRSMEVTPEQMLDDAYLKSR
                     AKLIDPKRATQFDFGMPKAGGTIYMSVADERGMMVSFIQSNYMGFGSGIVVPDSGISL
                     QNRGYGFSMDPKSANVVEGGKRPFHTIIPAFLTQQVDGKQEAVMSFGVMGGDMQPQGH
                     LQSIVRMLDYGQQPQAACDAPRWKVNRDFSVDVEATLDEDTVRALEGLGHTIKSVEDP
                     YMDFGSGQYIWKLDRNDPERGYVA"
BASE COUNT          354 a          611 c          630 g          360 t
ORIGIN      
        1 ggccacgcac ggatcatcgt gcgctacatc ctgccgaacg tgctgccgcc gatcatcgtg
       61 caggccagtc tgacgatcgc gaccgcgatc atcgccgaag cgagcctgtc gttcctcggg
      121 ctcggccaat tgccgccggc gccgtcgtgg ggttcgatgc tcaacaccgc gaaggatttt
      181 gtcagtcagg cgccgtggat gtcgattttc ccgggcatcg cgatctttct ggccgtgctc
      241 ggtttcaacc tgctcggcga cggtctgcgt gatgcgctcg atccgcgcga gtcgtaaccg
      301 gattccatca ccgcttcaat cggccgactg ccctctcatc actttcgttt gagttttcaa
      361 tgacacgttt caattggcaa aacccgtatc cgacaccccg cttgcccgtg ttcgcgcgca
      421 acatcgtttc gacgtcccat ccgcttgccg cccaagccgg cctgcgcatg ttgtggaaag
      481 gcggaaatgc catcgatgcg gcgattgccg cggccgcggc gatcacggtc gtcgagccgg
      541 tctctaacgg cctcggcagc gacgcgttcg cgctcgtctg ggacggcaag aagctgcatg
      601 gcctgaatgc ttccggcgtc tcgccggcgg cgtggaacgt cgattacttc aagcgcaagt
      661 acggcgagga aaacggtctc gcgaaccacc ccaagcgcgg ctgggatgcg gtgacggtgc
      721 cgggcgtgat cgccggctgg gaagcgttgc atcagaagtt cggctcgctg ccgttcgcgg
      781 atctgatgga gccggcgatc gagatcgccg aacgaggtca tgcagtggcg agcgtcgttg
      841 cgtacaagtg ggcggcggcc gtgcccgagc tgaagaacca gccgggtttc gccgacacgt
      901 tcatgccgcg cggccgggcg ccggaagtca gcgaactggt gcgctttccc ggtcacgcga
      961 agacgctgcg caggctcgct gaactaggtc cgcgcgcgta ctacgaaggc gagatcgccg
     1021 aacagatcgc cgcgttctcg cgccagggcg gcggcgcgct cacgatggac gacctgcgca
     1081 actaccgcgt cgactgggtc gagccgatca gcaaggacta tcgcggccac acggtgcatg
     1141 aaattccgcc gaacggtcag ggcattgccg cgctgatcgc gctcggcatt ctcgaacagt
     1201 tcgacgtggg ctcgctgccg gttgacggca tcgaatcgca gcacctgcag atcgaagcca
     1261 tgaagctcgc gttcgccgac gtctaccagt acgtcgccga tccgcgttcg atggaagtca
     1321 cgcccgagca gatgctcgac gacgcgtacc tgaagtcgcg cgcgaagctg atcgacccga
     1381 agcgtgccac gcaattcgac ttcggcatgc cgaaggcggg cggcacgatc tacatgtcag
     1441 tggccgacga gcgcggcatg atggtgagct tcatccagtc gaactacatg ggtttcggat
     1501 cgggcatcgt ggtgccggac tccggcattt cgctgcagaa ccgcggctat gggttctcaa
     1561 tggatccgaa gtcggcgaac gtggtcgagg gcggcaaacg accgttccat acgatcatcc
     1621 ctgctttcct gacccagcag gtggacggca agcaggaagc ggtgatgagc ttcggcgtga
     1681 tgggcggcga tatgcagccg caaggccatc tgcaatcgat cgtgcgcatg ctcgactacg
     1741 gccagcaacc gcaggccgct tgcgacgcac cgcgctggaa ggtcaatcgc gacttctcgg
     1801 tcgacgtcga ggcgacgctc gatgaggaca cggtgcgcgc gctcgagggt ctcggccata
     1861 cgataaagtc agtcgaggat ccgtatatgg acttcggctc cgggcaatac atctggaagc
     1921 tcgatcgcaa cgatcccgag cgcggttatg tcgcc
//
LOCUS       DATASH010000538         4900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2855112,
            whole genome shotgun sequence.
ACCESSION   DATASH010000538 DATASH010000000
VERSION     DATASH010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4900
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2855112"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VHV99_14935"
     CDS             complement(<1..183)
                     /locus_tag="VHV99_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009938729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636751.1"
                     /translation="MTNKTDARNLREAALDYHEFPTPGKIAIAPTKQMINQRDLALAY
                     SPGVAFACEEIVENPLN"
     gene            508..1422
                     /locus_tag="VHV99_14940"
     CDS             508..1422
                     /locus_tag="VHV99_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3636752.1"
                     /translation="MKTDIASDLEFFVLVARHGSLSAAARALDLTPPAATRRLVQMES
                     RLGVRLVNRTTRSVSLTGEGQTYLLHATRILAEVREMEDQVSSGRSTPRGLLRVNATL
                     GFGRTAIAPIVSEFARRYPDVAVQVEVTDRPIDLVESGFDLAIRFGLLPDKRLNARRV
                     MSNRRLLCASPAYLKRHGTPSSLQDLSDHRCIIHRQNDDAYGIWRFMHGEQSEVVKVH
                     GMLSSNDGDIVLGWALEGHGIVIRSEWDLAKYLESGRLKVVLPDFVLPSADLYVYYPS
                     RSNQTVRARVFIEFLVEHFQTPVPTRAE"
     gene            1748..2737
                     /locus_tag="VHV99_14945"
     CDS             1748..2737
                     /locus_tag="VHV99_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008345613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX3636753.1"
                     /translation="MNRTLTPRKLPVTIISGFLGAGKTTLVNQLLQQHAPGSIGVVVN
                     EFGEVGIDGQLIVAEEQALVEINNGCVCCTVRADLVASVKALLARPQTGAAPMQRLIV
                     ETSGLADPAPVLQTFLADPDLREAVDLESVVTVVDALHLRRQLDDDIAREQVAFADVI
                     LINKLDLITEDELIALERTIRSLNPTAAIITSTRSQIRADALLGVRRFSLPTLLAIEP
                     DLLDEGAHDHEHDTSIQSCALVEKGDLDATRFNRWINELVQQQGQQLMRMKGVLNFHA
                     EARQFHFHSVHMLLEAAPGRAWLKDEPRENKFVFIGRNLDFPQLRESFLSCIH"
     gene            2782..3285
                     /locus_tag="VHV99_14950"
     CDS             2782..3285
                     /locus_tag="VHV99_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HEX3636754.1"
                     /translation="MKKTDALTVSIGVLAVVDTYVTATVFPVPVWVTFIAWASFFVVG
                     GGPRGFVQSVASNLTGLVIASLSLLAIANTGPAPIVAAICVGLGSAAMVQVSKLKLLN
                     VLPAVVWGFASTVGTTVATGKAITTVGIQNPALVAAAALVTGAVFGIVSEVFGEALSH
                     KPQHQFN"
     gene            3311..4054
                     /gene="nthB"
                     /locus_tag="VHV99_14955"
     CDS             3311..4054
                     /gene="nthB"
                     /locus_tag="VHV99_14955"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007739952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit beta"
                     /protein_id="HEX3636755.1"
                     /translation="MKLQHSLGGLENLGPVNVETRVFVEPWEKRIFGIHTVMMAESAH
                     LSDARPQYPVATLPTTFKNTWTWASLRTGAEGMQPFEYFKYRYYEKWLMGISQFFIDQ
                     GYVCADELAQKTAHYRAHPDAPLPAQPNPAIASQINEYLAKGDSGLNALKQAPRFEPG
                     AEVCIADPAAVDHTRLPGYLRNKRGVIDLVYPGAYSYFVSTGPDGIGEPMPVYRVAFK
                     AEDIWGKDKSEPNTTIYADLYEAYLDASN"
     gene            4088..4729
                     /gene="nthA"
                     /locus_tag="VHV99_14960"
     CDS             4088..4729
                     /gene="nthA"
                     /locus_tag="VHV99_14960"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007739951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HEX3636756.1"
                     /translation="MSELFKYDDEREAASAAKVRALEALLIEKGVLGSDSVDTVLGHF
                     ETMAGPFNGAKIVARAWIDPAFRERLVEDTPKAIAEMDFPGGMEGAEGEHMAAVANGN
                     GVHNLIICTLCSCYPWPVLGLPPYWYKDPVFRARGVREPRAVLKEFGVPVSDDTEVKV
                     WDSSAQIRWFVIPERPAGTEGMSEAELATLVTPEAMMGVALVPSPSASPRPAV"
BASE COUNT          946 a         1528 c         1459 g          967 t
ORIGIN      
        1 gttcagcgga ttctcgacga tttcctcgca cgcgaacgcc acgcccggcg agtacgccag
       61 cgcgaggtcg cgctggttga tcatctgctt ggtcggggcg atcgcgatct ttccgggggt
      121 cgggaactcg tgataatcga gagcggcttc gcggaggttc cgtgcgtcag tcttgtttgt
      181 cattctggcg ttactcgcaa actatgtatg gtcggtacgg tcggatatcg agggcatcag
      241 aaatccatga cggcccagcg gggactgcgc cggcactggc gtcgcatcgt gcacggacgg
      301 cagcgccgat tgcgtgctgc gcatcgagat cgccggtcag ttaaattgcc gtgccgtcgg
      361 cattgggcgt atagccggtg ggcaattcat cacggcacct tctgaaacat tgcatgcggg
      421 tcaatgtacg gagaagtagg atccaatagc ttatccgcgg gatgcgggtt tagaattcac
      481 tccaaatcac ttgtttattc actttcagtg aaaactgaca tcgcttccga tcttgagttc
      541 tttgtgctgg tcgcacgcca cggcagcctc tcggcggcag cccgagcgct cgatctaacg
      601 ccgccggccg ccaccagacg acttgtgcag atggaaagcc ggctcggcgt gcgactcgtc
      661 aaccggacga cgcgcagcgt cagtttgacg ggcgagggcc agacatacct gctgcacgcc
      721 acgcgcattc tggccgaagt ccgcgaaatg gaagaccagg tgtcgagcgg ccgctcgaca
      781 ccacgcggcc tgttacgcgt gaatgcgacg ctcggcttcg gccgcacggc gatcgcgccg
      841 atcgtctccg aattcgccag gcgctatccg gatgtcgcgg tgcaggttga agtgactgac
      901 aggccaatcg atctggtcga aagcggcttt gatctggcca tccggtttgg cttgctgccc
      961 gacaaacgcc tcaatgcgcg acgtgtgatg tcgaaccggc gtttgctgtg cgcctcgccc
     1021 gcctatctca agcggcacgg cacgccgtcc agccttcagg atctcagcga ccaccgctgc
     1081 atcatccatc ggcagaacga cgacgcctac ggcatctggc gcttcatgca cggcgaacag
     1141 agcgaagtcg tgaaggtaca cgggatgctg tcgagcaacg acggagatat cgtgctcgga
     1201 tgggcgttgg agggccacgg gattgtgatt cgttcagagt gggatctggc caagtatctg
     1261 gagagtggca ggctgaaggt ggtgctgcct gatttcgtgc tgccgtcggc ggatctgtat
     1321 gtgtactatc cgagccgaag caaccagacc gttcgtgcgc gggtgttcat cgaatttctg
     1381 gtcgagcatt tccaaacgcc ggtcccgacg agagcggaat agccgtagac gccgtccgtc
     1441 gtcgcggaat ccgcgctttc attagcgctt tcatgggcgc ttttccagct ggtcatcttc
     1501 accgctccgc atattcgcgg agcattgcgc accatcgcgg cgcatcgaat ccccacatct
     1561 gcaccagcgc ttggcatcgg attcacgccg catcgccgtg ccattttgtc cttattttcc
     1621 gtcccggaca aaaaccgcca catttccagt gcattctgga caagctgagt tcccgcacag
     1681 acggatcacg gcgacaggcc tggtactcgc tctattgagg cagtctcgat cagcaacgga
     1741 ctctctcatg aatcgcacac tcacaccgcg caagcttccc gtcactatca tctccgggtt
     1801 tctcggcgcc ggtaaaacca ccctcgtcaa tcagttgctg cagcaacacg cgccgggatc
     1861 aatcggcgtg gtcgtcaatg aattcggcga agtcggcatc gacggtcaac tgatcgtcgc
     1921 cgaagaacag gcgctggtcg agatcaataa cggctgcgtg tgctgcacag tgcgcgcgga
     1981 cctcgtcgct agtgtgaaag cactgctggc gcgtccgcag accggcgcgg cgcccatgca
     2041 gcgcctgatc gtcgagacat ccggactggc cgatccggcg ccggtcctgc aaacgttcct
     2101 cgccgatccc gacctgcgcg aagccgtgga tctcgaatcg gtcgtcaccg tggtcgacgc
     2161 cctgcatctg cgccggcaac tcgatgacga catcgcgcgc gaacaggtgg ccttcgccga
     2221 cgtcattctg atcaacaagc tcgacctgat cacggaagac gagctgatcg ccctcgaacg
     2281 gacgatccgt tcattgaatc caaccgctgc catcatcacc tcgacccgtt cgcaaatccg
     2341 cgcggacgct ctgctgggtg tgcgccgctt ctcgctgccc acgctgctcg cgatcgagcc
     2401 cgatctgctc gacgaaggcg ctcacgacca cgaacacgac acgtcgattc aatcgtgcgc
     2461 gctggtcgaa aagggcgacc tcgatgcgac gcgctttaac cgttggatca acgaactcgt
     2521 gcagcagcaa ggccagcaac tgatgcgcat gaaaggggtc ctcaacttcc acgccgaagc
     2581 gcggcagttc catttccata gcgtgcatat gttgctggaa gccgcacccg gacgcgcatg
     2641 gctgaaggac gagccgcgcg agaacaagtt cgtgtttatc ggccgcaatc tggattttcc
     2701 gcaactacgc gaatcgtttc tcagctgcat ccattaaccg cacttacccc accttcccca
     2761 cttaccctca aggagtctgt catgaagaag actgatgcgc ttaccgtcag tatcggcgtg
     2821 ctcgccgtcg tcgacaccta cgtcaccgcc acggtatttc cggtaccagt gtgggtcacc
     2881 tttatcgcgt gggcgtcgtt cttcgtggtc ggcggtggtc cgcgcggctt cgtgcagagc
     2941 gtcgcgtcga acctcaccgg tctcgtgatc gcgtcgctct cgctgctggc gatcgccaat
     3001 accggcccgg cgccgatcgt tgcggcgatt tgcgtcggtc tcggcagtgc ggcgatggtg
     3061 caggtatcga aactgaagct gctgaatgtg ctgcccgctg tcgtgtgggg cttcgcttcg
     3121 actgtcggca ccacagtcgc caccggtaag gcgattacca cggtcggcat tcagaacccc
     3181 gccttggtgg ctgccgccgc gctcgtcacc ggcgccgtct tcggcatcgt ctccgaagtg
     3241 ttcggcgaag cactgagcca caagcctcag catcaattca actgacctca cgctccagga
     3301 accgcacacc atgaaactac agcacagcct tggcgggctc gaaaacctcg gacccgtcaa
     3361 tgtcgagaca cgggttttcg tcgagccgtg ggagaagcgc attttcggca ttcatacagt
     3421 gatgatggcg gaaagcgcgc atctgtcgga cgcgcggcca cagtacccgg tggcgacgct
     3481 gcctaccacc ttcaaaaata cgtggacctg ggcgtcgctg cgcaccggtg ccgaaggcat
     3541 gcagccgttc gaatacttca aataccggta ctacgaaaag tggctgatgg ggatttcgca
     3601 attcttcatc gatcagggtt atgtatgcgc cgacgaactc gcgcagaaga ccgcgcatta
     3661 ccgcgcccat cccgatgcgc cgctgcccgc acagccgaat ccggccattg cctcgcagat
     3721 caacgagtac cttgcaaaag gcgattcggg gctaaacgca ctgaagcagg caccgcgttt
     3781 cgaacccggc gccgaggtgt gcatcgccga tccggccgcc gtcgatcaca cgcgtttgcc
     3841 gggctacctg cgcaacaagc gcggggtgat cgacctcgtc tatccgggcg cctactccta
     3901 cttcgtttcg accgggcccg acggcatcgg cgagccgatg cccgtctatc gcgttgcatt
     3961 caaggccgag gacatctggg gcaaggacaa gagcgaaccg aacacgacga tctacgccga
     4021 tctgtacgaa gcgtatctcg acgcatcgaa ctgaatctct acatccatca tctggtacgg
     4081 aattaacatg agcgaacttt tcaaatacga cgacgagcgc gaggccgcga gcgcggccaa
     4141 ggtacgggca ctcgaagcgc ttctgatcga aaaaggggtg ctaggcagcg attcggtcga
     4201 taccgtgctc ggtcatttcg aaacgatggc tggcccgttc aacggcgcga aaatcgtggc
     4261 gcgtgcgtgg atcgatccag cgttcaggga acgcctcgtc gaagacacac cgaaggccat
     4321 tgcggaaatg gacttcccgg gcgggatgga aggcgcggaa ggcgaacaca tggccgcagt
     4381 cgccaacggc aacggcgttc acaacctcat catctgcacg ctctgctcct gctatccctg
     4441 gcccgtgctg ggtctgccgc cctactggta taaggacccg gtattccgcg cgcgcggcgt
     4501 gcgcgaaccg cgcgccgtgc tgaaggagtt cggcgtgccg gtttcggacg acacggaagt
     4561 caaggtatgg gacagcagcg cgcaaatccg ctggttcgtc atcccggagc ggcccgccgg
     4621 caccgaaggc atgagcgaag cggaactggc cacgctcgtc acgcccgaag cgatgatggg
     4681 tgtggcgctg gtgccctcgc cttctgcctc gccgcggccg gcggtctgat catgtttacc
     4741 cggttcgagg aatatgccgc ctccagcatg cttggacatc cggactctcc gccgcgcatg
     4801 cagggcaaac tgctgttcga caagccatgg gaacggcagg tattcgggct cgcgctttcg
     4861 ctgtctaagg cggggcactt cgagtgggat gacttccgtc
//
LOCUS       DATASH010000539         2510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5627427,
            whole genome shotgun sequence.
ACCESSION   DATASH010000539 DATASH010000000
VERSION     DATASH010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5627427"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(447..692)
                     /locus_tag="VHV99_14965"
     CDS             complement(447..692)
                     /locus_tag="VHV99_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012402368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636757.1"
                     /translation="MSEAVRIAGTAHAREDACAPRCASCRNRAEDRHSLEQSIPGLAV
                     FSSGFGASVADSRLCRLHDQLVSPDDVCAQYDPVPRH"
     gene            complement(689..2122)
                     /locus_tag="VHV99_14970"
     CDS             complement(689..2122)
                     /locus_tag="VHV99_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEX3636758.1"
                     /translation="MSDTNQTLPSGAAPSQPRSGHSAHGGGLFSTEDWWAVWVGLLVI
                     VIAWALFASGSSIKWLAVAPAKWSSVEQAAQEIGKHLPNYLALFVVFAVLFGVSLAAL
                     KQRVGAFLASFLVLFIASALIFTLGAWVNASKYNLEPPLVALALGLLISNVVTLPEWF
                     AAGLRVEFYIKVGIVLLGATLPFTLLVWAGPVAVGQATIVSLVTFFVIFFAGKALGLD
                     RRFAAVLGVGGAVCGVSAAIAIAGAVRAKREQASVAITLVVLWAIVMIFVLPFASRSL
                     GLSTAVAGAWIGTSEFADAAGIAAAQAYGDFAKHAGGVIAGAPEASLQAFTLMKVVGR
                     DIWIGIWAFVLAIVATTRWESQDSGVKAKPNAGEIWARFPKFVIGFVIASALVTWIAS
                     HYSLADYRKVVTPEFVAPITALRTWAFTFCFLSIGLTTRLRSLTTTGLKPFLAFTAGV
                     VVNIAIGYALSAHVFAPYWNSLGQGSS"
     gene            complement(2294..>2510)
                     /locus_tag="VHV99_14975"
     CDS             complement(2294..>2510)
                     /locus_tag="VHV99_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636759.1"
                     /translation="ALTEIPAAERVTVYDAVPLTIVKVMHVHRAQLLIESASRGALQA
                     TLRAWQPLLRGLKGVLRWNLEVDPLDI"
BASE COUNT          511 a          816 c          790 g          393 t
ORIGIN      
        1 ttgcgcgtcg cgatagcagt tgcgatcagt actttccctg gcgtgattct tttcgctttt
       61 acccttatca gatcgcccgg cgcgccgtta tacagaacat ggatggtcgc ggccgcaagg
      121 cacgcacata aaaacacggt acggggtttt agaggactgg cgaaatggaa aagtggttag
      181 tcgtattggg gatctgggcg atgtgcacaa cttgcgctgt gcttttcatc cgtggcgcga
      241 ccggtggccc gggcaagcgt gtgactgaag aaaggcgcgc gcgtcctgcg tcgcgtgcat
      301 cggctggcga tacacgggca gcgctgaacg attgaggggc gaggcccggc ggctaacgat
      361 cgacatcgag acacctttcc ccggctgaca ccggatagtg agcgcgcgcc gcctggttca
      421 ggcggctggc gctgcggacg tgcgcgtcag tggcgtggga ccgggtcgta ctgcgcgcaa
      481 acgtcgtcgg gtgaaacgag ctgatcgtgc agccggcata gccggctgtc cgccacgctc
      541 gcgccaaacc cggaactgaa cacagcgaga cccggaatgc tctgctccag tgaatgccga
      601 tcctcggcgc gattccggca gcttgcacag cgcggtgcgc atgcgtcttc gcgtgcgtgg
      661 gctgtgccgg cgatgcggac agcctcgctc atgacgaacc ctgccccaag ctattccagt
      721 acggtgcgaa cacgtgcgcg gacagcgcgt agccgatggc gatattgacc accacgccgg
      781 cggtgaaggc cagaaaaggc ttcagcccgg tggtcgtgag cgaacgcagg cgcgtggtga
      841 gcccgatgct caggaaacag aacgtgaagg cccacgtgcg caacgcggtg atcggtgcga
      901 cgaattcggg cgtgacgacc ttgcggtaat ccgccagcga atagtggctg gcgatccacg
      961 tcaccagcgc cgacgcaatc acaaagccga tcacgaactt cggaaagcgc gcccagatct
     1021 cacccgcatt gggtttggct ttgacgccgc tatcctgcga ctcccagcgc gtcgtcgcga
     1081 caatcgccag cacgaacgcc cagatgccga tccagatatc gcggccaacc accttcatca
     1141 gtgtgaacgc ctgcagcgat gcctcggggg cgcccgcaat cacgccgccc gcatgtttcg
     1201 cgaagtcgcc gtaggcttgc gcggcggcaa tgcccgcagc gtcggcaaat tcggacgtgc
     1261 cgatccaggc gcccgccacc gccgtcgaga gtcccagcga acgcgatgcg aacggcagca
     1321 cgaagatcat cacgattgcc cacaacacga ccagcgtgat tgccaccgat gcctgctccc
     1381 gctttgctcg cactgcgccg gcaatcgcaa tcgccgccga tacgccgcac accgcgccgc
     1441 ccacgccgag cacggccgca aaccgccggt cgaggccaag cgccttgccg gcaaagaaga
     1501 tcacgaagaa cgtgacgagc gagacgatgg tggcctgccc caccgcgacc ggccccgccc
     1561 acaccagcaa ggtgaacggc agcgtcgcgc cgagcagcac gatgccgacc ttgatgtaaa
     1621 actccacgcg caggccagct gcgaaccact cgggcagcgt gacaacgttc gagatcaaca
     1681 ggcccagcgc gagcgccacc agcggcggct cgaggttgta cttcgacgcg ttgacccacg
     1741 cacccagcgt gaagatcagc gccgacgcga tgaacaggac caggaacgac gccaggaaag
     1801 cgccgacgcg ctgcttcaac gcggcaaggc ttacaccgaa cagtacggcg aacacgacga
     1861 acagcgcgag atagttcggc agatgcttgc cgatctcctg cgcggcctgc tcaacgctcg
     1921 accatttggc cggcgccacc gccagccact tgatgctgct gcccgaggcg aacagcgccc
     1981 aggcaatcac gatcaccagc agtccgaccc agacggccca ccagtcttcc gtggaaaaga
     2041 gaccgccgcc gtgcgcgcta tggccgctgc gcggttggct gggcgcggcg ccgctcggca
     2101 gtgtctggtt ggtatcgctc atcgcaaatg ccctgggaaa gttaaggtgc atcggcgcgt
     2161 gattgttgag ttttgcctga atatgcgccg gttcggcgaa atataggcga gcggctccgc
     2221 aaagcgaaac gaccattcgc tctatgaata tgactgaaag gagtaacgct ctccgccgac
     2281 gcggcgagag gcgtcagatg tccagcgggt cgacttcgag attccagcgc agcacgcctt
     2341 tcaagccgcg cagcagcggt tgccacgcgc gcaaagtggc ttgcaacgcg ccacgcgacg
     2401 cgctctcgat cagcaattgc gcgcgatgca cgtgcatgac cttgacgatg gtgagcggca
     2461 cagcgtcata gaccgtcacg cgctcggcgg ccggaatctc cgtcagcgcg
//
LOCUS       DATASH010000540         2085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5627722,
            whole genome shotgun sequence.
ACCESSION   DATASH010000540 DATASH010000000
VERSION     DATASH010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5627722"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="VHV99_14980"
     CDS             complement(<1..480)
                     /locus_tag="VHV99_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018434626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3636760.1"
                     /translation="MSALHLLAVTLAYACAAGAVFGIAYTVLASALIGRFFARAVSEP
                     TSFPPVTIVKPLHGNEWALLTNLSSFCQQDYPGPVQFLFGVHDSADPALQTVDDLRKL
                     HPEADITVVADPRLYGPNRKISNILNMLPQARHDVLVFADSDVSVGSDYLRNVIGELQ
                     "
     gene            complement(477..1898)
                     /gene="hpnJ"
                     /locus_tag="VHV99_14985"
     CDS             complement(477..1898)
                     /gene="hpnJ"
                     /locus_tag="VHV99_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012735064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis associated radical SAM
                     protein HpnJ"
                     /protein_id="HEX3636761.1"
                     /translation="MKTLFLQAPSYDGFDGGAGSRYQAKREVRSFWYPTWLAQPAALL
                     PDSRVLDAPADGLSVDASLDIAQQYDLVVIHTSTPSFPSDALFAEDLKKRKPSMLIGM
                     VGAKVAVDPHNSLTASEAIDFVCREEFDFTCQEVAEGKPFAGIKGLSYRAPDGSIEHN
                     EARPILENMDELPFVAPVYKRDLKIYNYFIGYLKHPYVSIYTGRGCRSKCTFCLWPQT
                     VGGHRYRTRSVENVLAEVKWIRDNMPEVKEIMFDDDTFTDFKPRVEEIARGLGKLGVT
                     WSCNAKANVPYSTLKIMKENGLRLLLVGYESGDDQILLNIKKGLRTDIARRFSEDCRK
                     LGIKIHGTFILGLPGETQDTIQKTIEYAKDINPHTIQVSLAAPYPGTTLYKQAVENGW
                     LEENKVINLVSKSGVQLAAIGYPHLSRDEIYHQLENFYKRFYFRPSKIWEILREMLTS
                     WDMMKRRLREGVEFFRFLRAHQA"
     gene            1901..>2085
                     /locus_tag="VHV99_14990"
     CDS             1901..>2085
                     /locus_tag="VHV99_14990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636762.1"
                     /translation="MLQQAFDWRFQFSVGKLSELLPAGIGPGQKESRKYYGAKLKETL
                     REIRVAASPNASEGWCA"
BASE COUNT          433 a          601 c          629 g          422 t
ORIGIN      
        1 ctgcaactcg ccgatcacat tgcgcagata atccgacccg acgctgacgt cgctatcggc
       61 gaacaccagc acgtcgtgcc gggcctgcgg cagcatattg aggatattgc tgatcttgcg
      121 gttcggtcca tacaggcgcg gatcggcgac cacggtgata tccgcttcgg gatgcagctt
      181 gcgcagatcg tcgacggttt gcaaggcggg gtcggccgaa tcgtgcacgc cgaacaggaa
      241 ttgcacgggg cccggataat cctgttgaca gaagctcgac agattggtga gcagcgccca
      301 ctcattgccg tgcaacggtt tgacgatcgt gaccggcgga aaactggtcg gctccgacac
      361 cgctctggcg aagaaccggc cgatcaacgc actggccaac actgtgtagg cgatgccgaa
      421 caccgcaccc gccgcacacg cataggccag cgtgacggca agcagatgca gggcgctcac
      481 gcttggtgcg cgcgcaggaa gcggaagaat tcgacgcctt cacgcaagcg ccgtttcatc
      541 atgtcccagc tcgtcagcat ctcacgcaga atttcccaga tcttcgacgg acggaaatag
      601 aagcgcttat agaagttttc gagctggtgg tagatctcgt cgcgcgacag atgcggatag
      661 ccgatcgccg ccagttgcac cccggacttg ctgacgagat tgatgacctt gttctcttcc
      721 agccagccgt tttcgaccgc ctgcttgtag agcgtcgtgc caggatacgg cgcggcaagc
      781 gacacctgga tcgtatgcgg attgatgtcc ttcgcgtact cgatggtctt ctggatcgtg
      841 tcctgcgtct cgcccggcag cccgagaatg aaggtgccgt gaatcttgat gccgagcttg
      901 cggcaatctt cgctgaaacg ccgggcaatg tcggtacgca ggcccttctt gatgttcagc
      961 agaatctggt cgtcgccgga ctcgtaaccg accagcaata ggcgcaggcc gttttccttc
     1021 atgatcttca gcgtcgagta cggcacgttc gccttcgcgt tgcatgacca cgtcacgccc
     1081 aatttcccaa gaccacgggc gatttcctcg acgcgcggct tgaagtcggt gaaggtgtcg
     1141 tcgtcgaaca tgatctcctt gacttcaggc atgttgtcgc gaatccactt cacttctgcg
     1201 agcacgttct cgaccgaacg ggtgcggtag cgatgcccgc ccaccgtttg cggccacagg
     1261 cagaacgtgc atttcgaacg gcaaccgcgg cccgtgtaaa tagacacgta ggggtgtttc
     1321 agatagccga tgaaatagtt gtagatcttc aggtcgcgct tgtagacggg cgccacgaag
     1381 ggcagctcgt ccatgttctc gagaatcgga cgcgcttcat tgtgctcgat cgaaccatcg
     1441 ggggcgcgat agctgagacc tttgatcccg gcgaacggct taccctcggc aacttcctgg
     1501 caggtgaagt cgaattcttc acggcagacg aaatcgatcg cctcgctcgc ggtcagagaa
     1561 ttatgtggat cgaccgcgac cttcgcaccg accatgccga tcagcatcga aggcttgcgt
     1621 ttcttcaggt cttcggcaaa gagcgcatcg ctgggaaacg acggtgtgct ggtgtgaatg
     1681 accaccagat cgtattgctg tgcaatatcg agcgatgcgt cgacggagag accgtcggcg
     1741 ggggcgtcga gtacgcggct gtcgggtaag agggcggcgg gctgcgcgag ccacgtcgga
     1801 taccagaacg agcgcacttc acgcttggcc tggtagcgcg aacccgcgcc accatcgaag
     1861 ccgtcgtacg acggcgcctg taaaaacagg gtcttcatga atgctccagc aagcctttga
     1921 ttggcgcttt cagttttctg tcggaaaact atcggaacta ttacccgctg ggataggccc
     1981 ggggcaaaag gaatcgcgca aatactacgg agcgaagctg aaggaaacct taagggaaat
     2041 acgtgtcgcc gcgtcgccca acgcctcgga gggttggtgc gcgag
//
LOCUS       DATASH010000541         1740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2066399,
            whole genome shotgun sequence.
ACCESSION   DATASH010000541 DATASH010000000
VERSION     DATASH010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2066399"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(241..1443)
                     /locus_tag="VHV99_14995"
     CDS             complement(241..1443)
                     /locus_tag="VHV99_14995"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013337754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEX3636763.1"
                     /translation="MNTVTLKPSKEKSLLRRHPWVYANAIDRVDGNPAAGATVLVRAH
                     DGRFLARAAYSPHSQIRARVWSFDEAEPIDHAFFKRRVQRALAHRQTMVHDTGAVRLI
                     FGEADGLPGLIVDHYVAEDEGQRSQLVCQFMATGVEAWKEAIVAALVGATGCPNVYER
                     SDVSIRQKEGLEQITGVLAGDAPSDALIASENGVRYHVDVRNGHKTGFYVDQRDNRLL
                     VQELAKDRDVLNCFCYTGGFSLAALKGGAKRVVSIDSSGDALAIAQQNVAANGFDAER
                     ATWLDADAFKTLRRLYDEGERFDLVVLDPPKFAPSREHVDRASRAYKDINLTGMKLLR
                     PGGLLFTYSCSGAIDSELFQKIVSGAAADARVDARILKRLGAGVDHPLLTAFPEGEYL
                     KGLLLQIA"
     gene            complement(1543..>1740)
                     /locus_tag="VHV99_15000"
     CDS             complement(1543..>1740)
                     /locus_tag="VHV99_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX3636764.1"
                     /translation="PANVHPHVLRHSFATHVLQSSGDLRAVQELLGHASITATQVYTA
                     LDFQHLAHVYDQAHPRAKKRD"
BASE COUNT          324 a          535 c          582 g          299 t
ORIGIN      
        1 taagcgcgaa tttaacgcgc gaatctaaag agcgaactta ccgcgcgaaa tgtgcagcgc
       61 gggatctgca gcgcgcaagc atgcaggaaa tgcgtctgcg aatccgccct gccgggcgcg
      121 gcagcctgac ggcgcgcgac ccgatctagc cggatattga aacatatctg tcaaaccggc
      181 gctgcgatgg gagcgaaccc ctctcctgcg cgtgggaaat gaccctgaaa ctatgggcga
      241 tcaggcaatt tgcaacagca ggcctttcag gtattcgcct tcggggaatg cggtgagcag
      301 cgggtggtcg accccggcgc cgaggcgctt gagaatccgc gcatcgacgc gcgcatcggc
      361 cgccgcgccg gacacgatct tctggaacag ctcggagtcg atcgcgccag agcatgaata
      421 ggtgaacagc aggccgcccg ggcgcagcag tttcatgccg gtcagattga tgtccttata
      481 ggcacgcgag gcgcggtcca cgtgttcacg cgatggcgcg aatttcggcg gatcgagcac
      541 gaccaggtcg aagcgttcgc cttcgtcgta gaggcggcgc agggttttga acgcgtcggc
      601 atcgagccag gtggcgcgct cggcgtcgaa accgttggcg gccacgttct gttgcgcgat
      661 cgcgagcgcg tcgcccgacg aatcgatcga caccacgcgc ttcgccccgc ctttcaatgc
      721 cgccagcgaa aagccgccgg tgtagcagaa gcagttcagc acgtcgcgat ctttcgccag
      781 ctcctgcacc agcaggcggt tgtcgcgctg gtcgacgtag aaaccggttt tgtggccgtt
      841 gcgcacgtcg acgtggtaac gcacgccgtt ttcgctcgcg atcagcgcgt ccgacggcgc
      901 gtcacccgcc agcacgccgg tgatctgctc gagcccttcc ttctggcgga tcgacacgtc
      961 cgaacgttcg tagacgttcg ggcagccagt tgcgccgacc agcgcggcga cgatcgcttc
     1021 cttccatgct tcgacgcccg tcgccatgaa ctgacacacc agctggctgc gctggccttc
     1081 gtcctcggcc acgtagtgat cgacgatcag accaggcagg ccgtccgctt cgccgaaaat
     1141 cagccgcacg gcgccggtgt cgtgcaccat cgtttgacga tgcgccaagg cgcgctgcac
     1201 acggcgcttg aagaacgcgt gatcgatcgg ctcggcttcg tcgaaactcc acacgcgggc
     1261 gcgaatctgc gaatgcgggc tgtaggccgc acgcgcgagg aagcggccgt cgtgcgcgcg
     1321 caccagcacg gtcgcgccgg cggcgggatt gccgtcgacg cgatcgatcg cgttggcata
     1381 gacccacgga tggcggcgca gcagggattt ttctttcgac ggtttgagcg taacggtatt
     1441 catcagggtg tcagcaggag cagtgggccg tgtggactaa gaagcaagcg gcgggtgaag
     1501 cgaaggcgga gactgcaaac cgcaacaagc aaacgagcgg aatcagtcgc gttttttggc
     1561 gcgcggatgc gcctgatcgt agacgtgcgc gagatgctgg aaatcgagcg cggtgtagac
     1621 ctgcgtggcg gtgatgctgg cgtgcccgag cagttcctgc acggcccgca ggtcgccgct
     1681 cgactgcagc acgtgcgtgg caaacgaatg ccgcagcacg tgcgggtgca cattggcggg
//
LOCUS       DATASH010000542         7371 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4639160,
            whole genome shotgun sequence.
ACCESSION   DATASH010000542 DATASH010000000
VERSION     DATASH010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7371
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4639160"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="VHV99_15005"
     CDS             complement(<1..370)
                     /locus_tag="VHV99_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX3636765.1"
                     /translation="MRPASLPVEIAGHPVVLSTLRAMFDPALRCLFVADVHFGKDAVF
                     RARGIPVPVGSTADNLMRLDILIAAFEPAMLMFLGDLLHAREAHAKETLDALQVWRAR
                     HAGLRVVLVEGNHDRHAGTLP"
     gene            complement(367..3012)
                     /locus_tag="VHV99_15010"
     CDS             complement(367..3012)
                     /locus_tag="VHV99_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response DEXH box
                     helicase"
                     /protein_id="HEX3636766.1"
                     /translation="MTEPADSDDNASPASSVSSERRRAPRPRRIPKSREQQARLDARE
                     AAFEPIPFAVDEAATRRPFAERLDAWFEARRWQPFDFQREVWREIGRGVSGLLHATTG
                     AGKTWAVWFGALAAFAAAPPSARNRAPRAQPDPLTVLWITPMRALAADTARALQSSAA
                     DLAVPWSVGLRTGDTSPAERTRQNRRMPSALVTTPESLSLILTRPDARAVLSHVRLVI
                     VDEWHELLSNKRGTQTQLALARLAHWRPEIQVWGLSATLGNLPFAAEVLLASVRTPRV
                     SVQGALPKALIVDTVIPETIERFPWGGHVGMRQVGAVADAISEAQTSLVFTNTRSQCE
                     VWYQALLDARPEWAGLIALHHGSLDQQVREWVERGLKNGMLKAVVCTSSLDLGVDFLP
                     VERVFQIGSPKGVARLMQRAGRSGHAPGRPSRVTIVPTHALELVEAAAAREAVEKRQI
                     EGRETPEKPFDVLVQHLVTVAIGGGFDAQELYGEIRCTYAYRQLTQEEFDWALGFVEG
                     GGTALRAYPDYHRVVRGSDGLYRVPREDLMRRHRNNIGTIVANGTLHVAYLSGGRIGA
                     IEESFISRLKPGDIFTFGGRALELIRVQDMTAWVRRAASSRGAMPQWAGSRMPLSSEL
                     ADATLTMLARAADGIYDEPEMRAVRPLLELQQKWSALPEPGVLVVELVKSREGHHFFC
                     YPFAGRTAHIGLGALLAWRVARDKPGTFSISMNDYGFELLSAQPFDWAAALESGLLSR
                     DELEHDILASLNSSELSMRRFREIARVSGLVFQGHPGQQKSARQLQASSGLFYEIFRN
                     HDSGNLLLNQADTEVLLQELDAQRIRIALERMNASRVVVTRPKKPTPFAFPLIVGRLR
                     EKVSTEKLADRVERMLAELEKAART"
     gene            complement(3080..4369)
                     /locus_tag="VHV99_15015"
     CDS             complement(3080..4369)
                     /locus_tag="VHV99_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018439588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEX3636767.1"
                     /translation="MTTENLEPELVVEDVAGRSWMKRLGPGLITGAADDDPSGIATYS
                     QAGAQFGFGLLWTLLITYPLMVSIQLVSARMGRVTGRGLATNLRHHYPAPLLYLAVLL
                     LLVANTINIAADLAAMGAAAKLVIGGPVQLYVALLGTASLLMQVFIPYERYASVLKWL
                     TLTLFAYVAIIFVIPIDWKAVGLSIVKPPIQFSGGYLTTVVAVLGTTISPYLFFWQAS
                     QEVEEIRSKPAEQPLLRAPLQASAQLKRINTDTWVGMAVSNSVAFFIMLTAAATLHVH
                     HVEIKTTADAATALKPIAGNLAFALFSLGIIGTGLLALPVLAGSAAYAAAGTMKWRNS
                     LALQVNLAKQFYGVIAVAIAGGIALTFMRLDPIKALYWSAVINGIAAVPIMALVMVMA
                     ASPKVMGQFAVTGWLRWLGWLATVAMGLAAVGMFWPT"
     gene            4587..4985
                     /locus_tag="VHV99_15020"
     CDS             4587..4985
                     /locus_tag="VHV99_15020"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018423488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX3636768.1"
                     /translation="MKSIVALVAAAVLSSTAMAASTTEKLPSGVIVEHLTQGTGAQPA
                     ADDVVKVNYRGTLANGTEFDSSAKHGGPATFPLNRVIPCWTQGVQKMKVGGKAKLTCP
                     AATAYGDRGVGVIPPNSDLTFEVELVGIVK"
     gene            5421..6494
                     /locus_tag="VHV99_15025"
     CDS             5421..6494
                     /locus_tag="VHV99_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013342679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6765 family protein"
                     /protein_id="HEX3636769.1"
                     /translation="MNIDFHYGVVYIVARVGGMTAGEAVTVAHACQYVDDATTSGILR
                     FAGGETFERFATAHKLFDYTNTENDQNRLVWTPFHFLPAGVGETLDEKAVCRPDSTVA
                     REVVRRAIRQRGTDTGLHRLGVALHTYVDTWAHQGFSGIESPANRVHLLEAEDCTREG
                     WLVRLTRATRHLVERIEEDVLTLALPVGHGAALHYPDQPWAKWHYVDGRNVSVKRHNL
                     PEFMQAAEMTCRAVRAYVAGREDFEYQPGLPADVNAALTRLLDTNRNLNDSKRLQTIC
                     EAVKTDAIPGLSESIPDYVAKGLGSWKYKATGLQSDDDSGDRPRWSDAFEKSDYRRFH
                     DAVKQHRFVTTQEILPACGLRIA"
     gene            complement(6505..6741)
                     /locus_tag="VHV99_15030"
     CDS             complement(6505..6741)
                     /locus_tag="VHV99_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636770.1"
                     /translation="MKAGFINTALAAVALISATAGTANAAGCMKGAVVGGVAGHYAGH
                     HAVAGAMAGCLVGRHMAKKQAEERAAQHQQVKVQ"
     gene            6850..7344
                     /locus_tag="VHV99_15035"
     CDS             6850..7344
                     /locus_tag="VHV99_15035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018423484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636771.1"
                     /translation="MKQRREIGYTGLRAKKMNSRPLNPRMRAYSRSGNSRIAQRRPFL
                     DVDDGETEMTPSNAAIYQAQASTPESLRTLAGYLELALDEGESVILMRIGTQVRSVYV
                     GNPSGTLEDLTDHGVVAAFQADEMLALTRLGLNRITADDQQYRFMRSVRYIADRQAVV
                     FTPT"
BASE COUNT         1466 a         2290 c         2243 g         1372 t
ORIGIN      
        1 ccggcaacgt ccccgcatgc cgatcatgat ttccttcgac cagcaccacc cgcagccccg
       61 catgccgcgc gcgccagacc tgcaacgcgt cgagcgtctc cttcgcatgc gcctcgcggg
      121 cgtgcaataa atcacccaga aacatcagca tggccggctc gaacgcggcg atcaggatat
      181 cgaggcgcat gagattgtcg gcggtcgaac cgaccggcac cggaatcccg cgcgcgcgaa
      241 acacggcgtc cttgccgaaa tgcacatcgg cgacaaacag acagcgcagc gccggatcga
      301 acatcgcgcg caaagtcgac agcacgacag gatgcccggc gatttcaacc ggcagcgaag
      361 caggcctcat gtccgcgccg ccttctcgag ttcggccagc atccgctcga cgcgatccgc
      421 gagcttctcc gtgctgacct tctcgcgcaa gcgtccgaca atcagcggaa aggcgaacgg
      481 cgtcggcttc ttcggccggg tgacgaccac gcggcttgca ttcatacgct cgagcgcgat
      541 acgaatgcgc tgggcatcga gttcctgcag caatacttca gtgtcggcct gattgagcag
      601 tagattgccg ctatcgtgat tgcggaaaat ctcgtagaag aggccactcg aagcctgcaa
      661 ttggcgcgcg cttttctgct gtccgggatg cccttgaaac accaaccccg acacgcgcgc
      721 gatctcccga aagcgccgca tcgagagttc ggatgaattg aggctcgcaa gaatgtcatg
      781 ctcgagttca tcgcgcgaca atagcccgct ctccaacgcc gctgcccaat cgaatggttg
      841 cgcggacaac aactcgaacc cgtaatcgtt catggaaatg gaaaaggtgc cgggcttgtc
      901 acgggccacc cgccatgcga gcaacgcacc caaaccaata tgcgcggtgc gccccgcgaa
      961 cggataacaa aagaagtggt gtccttctcg cgatttcacc aattccacca ccagtacgcc
     1021 cggctcgggt aacgccgacc acttctgctg caattcgagc aacgggcgca cggcgcgcat
     1081 ttccggttcg tcgtagatgc cgtcggcggc gcgcgcgagc atcgtcagtg ttgcatcggc
     1141 gagttcggaa gaaagcggca tgcggctgcc tgcccattgc ggcatggctc cgcgtgaaga
     1201 ggcagcgcga cgcacccacg ctgtcatatc ctgcacgcga atcaattcga gcgcgcgtcc
     1261 gccgaaagta aagatatcgc ccggctttag ccgcgaaata aacgattcct cgatcgcacc
     1321 gattcgcccg cccgacagat aagcaacgtg caaggtgccg ttcgcgacga tcgtcccgat
     1381 gttgttgcga tgccggcgca tcagatcctc gcgcggcacg cggtacagtc cgtcgcttcc
     1441 acgcaccacg cgatggtagt cggggtacgc gcgcaatgcc gtgccgccgc cttcgacaaa
     1501 accgagcgcc cagtcgaatt cttcctgcgt cagttgccgg tacgcatagg tgcaacgaat
     1561 ttcgccatac agttcctgcg catcgaagcc gccgccgatc gccacggtga cgagatgctg
     1621 cacgagcacg tcgaacggtt tctccggcgt ttcgcggcct tcaatctgac gcttttcgac
     1681 ggcttcgcgc gccgcggcgg cttcgacgag ttcgagcgca tgcgtcggca cgatggtcac
     1741 gcgcgacgga cggcctggcg cgtgcccgga gcgtcccgcc cgttgcatca gacgtgcgac
     1801 gcccttcggc gaaccgattt gaaacacgcg ttccacggga aggaaatcga cgccgagatc
     1861 gaggctcgaa gtacacacaa ccgctttcag catgccgttc ttcagaccac gttccaccca
     1921 ttcgcgcact tgctggtcga gtgagccgtg atgcaacgcg atcaggccgg cccattcggg
     1981 ccgcgcatcg agcagcgcct gataccagac ttcgcattgc gagcgggtgt tggtgaaaac
     2041 cagcgaggtc tgtgcctcgc taatcgcatc ggccacagcg ccgacctggc gcatgccgac
     2101 atggccgccc cacggaaacc gctcgatcgt ctcgggaatc acggtatcga cgatcagcgc
     2161 cttaggcagc gcaccctgca cgctgacgcg cggcgtcctg acagacgcga gcaagacttc
     2221 ggcggcgaag ggcagattgc cgagggtcgc ggacaagccc cacacctgta tttcaggccg
     2281 ccaatgcgcg agccgcgcga gcgcgagttg cgtctgcgta ccgcgtttgt tgctgagcaa
     2341 ctcatgccat tcatcgacga tcaccagccg cacatgcgag aggaccgcac gcgcatcggg
     2401 ccgcgtgagg atcagcgaca ggctttcggg cgtggtgacg agtgccgacg gcatgcgccg
     2461 gttctgacgc gtgcgttccg ctggcgaggt gtcgccggta cgcagcccga cgctccatgg
     2521 cacggcgagg tcggccgccg agctttgcaa ggcgcgcgcg gtgtccgcgg cgagcgcgcg
     2581 catcggcgtg atccatagga cggtgagtgg gtcgggttgc gcgcgcggcg cgcggttccg
     2641 ggcggagggc ggtgcagcag cgaaggcggc aagggcgccg aaccagaccg cccacgtttt
     2701 gcccgcgccg gtggtggcgt gcaacaaacc gcttacgccg cggccgatct ctcgccatac
     2761 ctcgcgctga aagtcgaatg gctgccagcg ccgcgcttcg aaccatgcgt cgagtctttc
     2821 agcgaatggg cggcgagtgg cggcttcgtc gacggcaaat ggaatcggct cgaaagccgc
     2881 ttcgcgtgca tcgagccgcg cttgctgctc acggctcttc ggaatccggc gcggccgcgg
     2941 cgcgcgacgc cgctcggacg aaacagagga tgccggactg gcgttgtcat cggagtcggc
     3001 gggctcagtc atcgagagcg ctctttagca tcggatttgc acgccggcgc gaagacgtcg
     3061 aataaacccg cacgcgccat cacgtcggcc agaacatgcc gacggccgcc agccccattg
     3121 cgacggtagc gagccagccg agccagcgca accagccagt cacggcgaac tggcccatga
     3181 ccttggggct ggccgccatc accatcacca gcgccatgat cggcacggcc gcgatgccgt
     3241 tgatcaccgc gctccaatac agcgctttga tcggatcgag ccgcatgaac gtgagcgcaa
     3301 tgccgccggc aatcgcgacg gcgatcacgc catagaactg cttcgccaga ttcacctgca
     3361 acgccaggct gttgcgccat ttcatggtgc ccgctgccgc ataggcggcc gaacccgcca
     3421 gcaccggcaa agccagcaga cccgtgccga tgatgccgag actgaacagc gcaaacgcca
     3481 ggttgccggc aatcggcttg agcgcagtgg cggcatcggc ggtggttttt atctcgacgt
     3541 gatgcacgtg cagcgtggcg gcggccgtga gcatgatgaa aaacgccacg ctgttcgaca
     3601 ccgccatgcc gacccacgtg tcggtattga ttctcttcag ttgggcggag gcttgcaacg
     3661 gcgcgcgcaa tagcggttgc tctgccggtt tcgagcggat ctcctcgact tcctgcgacg
     3721 cctgccagaa gaacagatat gggctgatcg tcgtcccgag tactgcaacc acggttgtca
     3781 gatagccgcc ggagaattga atcggcggtt tcacaatact cagaccgacg gctttccaat
     3841 cgatcggaat cacgaagatg atggcgacgt aggcgaacaa cgtaagcgtg agccatttca
     3901 gaacgctcgc atagcgttcg tatggaatga atacctgcat cagcagggac gccgtgccga
     3961 gcaacgcgac gtagagttgg accggaccgc cgatcaccag tttggcagcg gcgcccattg
     4021 ccgcgagatc ggccgcgata ttgatggtgt tggccacgag cagaagcaat actgcgagat
     4081 acaaaagcgg agcggggtag tgatggcgca ggttggtggc gagtcctctg cccgtcaccc
     4141 gtcccatgcg cgcactgacg agctgtatcg acaccatcag cgggtaagtg atgagcaacg
     4201 tccacaacag tccgaaacca aactgcgcgc cggcctgcga ataggtggcg atgccgcttg
     4261 gatcgtcgtc cgcggcgccg gtgatcaggc caggccccag ccgcttcatc caggagcgtc
     4321 cggcgacgtc ctcaacgaca agttccggtt ccaggttttc tgtggtcatc gtatccgccg
     4381 gtcagggaag ctatgtcgaa tcatgtcgta cgggcgctat tgccgggacg agatggtcgt
     4441 ccgttcttca gtagatagca ttctctgtgc cacccgcctt ggcgcccagt tgccattgcc
     4501 tctgcgatag cctgcatcgc tgagcggcgg cctctggacg ttgccatccg ctacaatgac
     4561 gcgcactacc caccaggaga ttcgctttga aatctatcgt tgctttggtt gccgcggctg
     4621 ttctttcctc gacggcgatg gccgcgtcca ccactgagaa gctgccttcc ggcgtaatcg
     4681 ttgaacatct tacgcagggc acgggtgctc aaccggccgc tgatgatgtc gtgaaggtca
     4741 actatcgcgg cacgctcgcc aacggcaccg agttcgatag ctcggcgaag catggcggtc
     4801 cggcgacctt tccgctcaat cgcgtgattc cctgctggac acaaggcgtg caaaaaatga
     4861 aagtcggcgg caaagccaag ctgacctgcc cggcggccac ggcttacggc gatcgcggtg
     4921 tcggcgtgat tccgcccaat agcgacttga cgtttgaagt cgaactggtt ggtatcgtca
     4981 agtaatacgg cgccaagccc gtgagactga gccccgctcc ggataacgaa gcggggtttt
     5041 tctttttctg ggctattaca tctattcaat cgcctggtaa ttccaaaaga tctgaaagaa
     5101 gagaagttct ggctttaccc ggctagtgcg aatagcatcg acacatccac gatagtgaaa
     5161 acgacgacaa tgccgtggcc gcgctattcg tcagcgcaac ccaccgtgta ttcgccatac
     5221 gggtcgatct gacggattca tcgcctgata tgaccggcgg cagcgtggct cactcgctcg
     5281 cgctgattgc gggcggcgtc tttttaaaag acccgcgcat tcggtcgttt accttctacg
     5341 ggggcgtcgg cgctgcaata gccggcttgt gcattccgag agatccgcac gtcattcgtt
     5401 gcgcgcgagg agcgcctgtc atgaacatcg actttcacta cggtgtggtc tatatcgtcg
     5461 cccgtgtcgg cgggatgacg gccggcgaag cagttacggt ggcgcatgcc tgccagtacg
     5521 tcgacgatgc gaccacttca ggaatactgc gttttgcggg tggcgagacg tttgaacgct
     5581 tcgccaccgc gcacaagcta ttcgactata ccaatacgga aaacgaccag aaccggctgg
     5641 tatggacgcc atttcatttt cttcccgccg gtgttgggga aacgcttgac gaaaaggcgg
     5701 tgtgccggcc ggatagcact gttgctcgcg aggtggttcg ccgggcaatc cggcagcgcg
     5761 gcaccgacac gggattgcac cgtctgggcg tggcgcttca cacgtacgtc gacacatggg
     5821 cccatcaggg attctccggt atcgaaagcc cggcgaaccg cgttcatctg ctggaggcgg
     5881 aggattgcac gcgggaaggc tggctggtcc gccttacccg cgctacgcgg catctggtcg
     5941 agcgcatcga agaagatgtg ctgacgcttg cattgcccgt gggtcacggc gctgctttgc
     6001 actatcccga tcagccgtgg gcgaaatggc attacgtcga cggcagaaat gtgtccgtca
     6061 aaaggcataa tctgcctgag ttcatgcagg cggccgagat gacgtgccgc gcggtgcgcg
     6121 cctatgtcgc ggggcgggaa gattttgaat accagcccgg tctgccggca gatgtcaacg
     6181 ccgcactcac caggcttctc gacaccaatc ggaacctgaa cgacagcaaa cgtttgcaga
     6241 ccatctgcga agcggtgaaa accgacgcca ttcctgggct gtcagagtcg attccggact
     6301 atgtggccaa ggggctcggt tcctggaaat acaaggccac cggtttgcag tccgacgacg
     6361 attccggcga ccgcccgcgt tggagcgatg cctttgagaa aagtgattac cggcgctttc
     6421 acgacgcggt caaacagcac cgtttcgtga ctacgcagga gattctgccg gcttgcggac
     6481 tgcggatcgc gtgaaccggc tgggctattg caccttcact tgctgatgct gcgcggcccg
     6541 ttcttccgct tgtttcttcg ccatgtgtct tcccacgagg cagccggcca tcgcgcccgc
     6601 cacagcatgg tgcccggcgt agtggccggc gactccgccc accaccgcgc ctttcataca
     6661 gccggccgcg ttcgccgtgc ctgctgtggc cgaaatcagt gctaccgccg cgagggcggt
     6721 attgataaaa ccagctttca tctgaaagta ccctgctatt gagttgtcga aaccatccga
     6781 atgcaactgg gttgccactg ctttgcttct cagtcctgtt cggattgtaa aaggacagtt
     6841 cgcggacagg tgaaacagcg tcgcgagatt ggttacaccg gattacgggc caaaaaaatg
     6901 aacagccgcc ctttgaatcc acggatgcgc gcctatagta gaagcggcaa tagccgcatt
     6961 gcacagcggc ggccttttct tgacgtggat gacggggaaa cagaaatgac gccatcgaac
     7021 gctgcgatct atcaggccca ggccagtacg ccggagtcgt tacgaacgct tgccggctat
     7081 ctcgaactgg cgctcgacga aggagaaagc gtgattctga tgcggattgg cacgcaggtg
     7141 cgcagtgttt atgtcggcaa tccgtccggc acgctggagg atttgacgga tcatggcgta
     7201 gtcgccgctt ttcaggccga cgagatgctg gcattgaccc ggttgggact caaccgcatc
     7261 actgccgacg atcagcagta ccgcttcatg cgcagtgtgc gatatatcgc cgaccgccag
     7321 gccgtcgtgt ttacgcccac gtgagcggcc ggccaggaat cggaaagaca a
//
LOCUS       DATASH010000543         8061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3054553,
            whole genome shotgun sequence.
ACCESSION   DATASH010000543 DATASH010000000
VERSION     DATASH010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 8061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8061
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3054553"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1114)
                     /locus_tag="VHV99_15040"
     CDS             complement(<1..1114)
                     /locus_tag="VHV99_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018432509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HEX3636772.1"
                     /translation="MDQPTLDLTHRASRAATARRVGVDVDAVRRTSRIAVRRTSRLLV
                     RLVVNVALWVAGGIVLLPAAAQAQDAGGPIVIPGPGEKNPAALADLAEQMQATPAKRA
                     GSAATLAAATAQSLTPGPVARDADPFTRAANASGSIVIPGAGEPPAAPRMIRTSFRPD
                     ANGVVTIPAPGNANAASANSAAAVARVNLMQGSRQVVPVVVTPAPALNAGRQGTGVQP
                     VAANAPGSGVESAIGNAPAAGGFDSLASRGEPPQLGANGKPIVTTAAAAKGAPTPVSM
                     LAAPATLARTPSMRQVAPAVAQQNLRPGAAAVAQPVPLAGQEDAEAIRSAALAFLQQQ
                     SAGLPGKVEITVAPAFPRGLAACTTLEPFMPSGARLW"
     gene            1342..1836
                     /gene="flgB"
                     /locus_tag="VHV99_15045"
     CDS             1342..1836
                     /gene="flgB"
                     /locus_tag="VHV99_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HEX3636773.1"
                     /translation="MLDKLDAEFAFGRQALDVRAYRQELLSSNIANADTPGYKARDVD
                     FASSLAGALKKTGGGAATGASNNSTLAMTQPAGVTSGMSMATTAPGHMSGNATLTPTG
                     GTPDDYGNLQYRIPTQPALDGNTVDIDTERVQFADNTVHFESGMTVLSNQIKTMLSAI
                     TSGS"
     gene            1989..2414
                     /gene="flgC"
                     /locus_tag="VHV99_15050"
     CDS             1989..2414
                     /gene="flgC"
                     /locus_tag="VHV99_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006402757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HEX3636774.1"
                     /translation="MPSLMNIFGVAGSAMSAQSQRLNVTASNLANADSTTGPDGQPYR
                     AKQVVFAVSPLGGARTGSGQQIGGVQVTGVVDDPTPMKTAYDPGNPAANPDGYVTLPN
                     VDPVQEMVNMISASRSYQANVETLNTAKSLMLKTLTIGT"
     gene            2425..3138
                     /locus_tag="VHV99_15055"
     CDS             2425..3138
                     /locus_tag="VHV99_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HEX3636775.1"
                     /translation="MTTNTTIGNNGTTLSQTLLDTMNGTKSATSSTSSASSSSASSLQ
                     STFLQLLVTQMQNQDPTNPMDSSQMTSQLAQINTVSGISQLNTTLTSLASQLSSSQEP
                     QAASLIGATVLTPGTSVSVASGQAGAFGVQLANSVSDLQVVVKNSSGQIVNTIDLGPQ
                     SAGTVPIGWTPTDTTGTTLPNGTYTISAVGTINGQPATATTLTGSTVESVVSQSGGTP
                     GLVLSNGSTVGLSSVAAII"
     gene            3182..4738
                     /locus_tag="VHV99_15060"
     CDS             3182..4738
                     /locus_tag="VHV99_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HEX3636776.1"
                     /translation="MGYEQGLSGLQAASSDLDVIGNNIANSNTVGFKSGSAQFADMYA
                     NSVATAVGNQIGIGTQLADVQQQFSQGTITTTNDALDVAINGNGFFQMSSNGSLVYSR
                     NGVFQLNNTGGITNSQGLQLMGYAANSSGVINTAQTVPLTVPTANIAPQPSTTITAGL
                     NLNAQDPLMLGQPNVTSALGAGSGLTTPGATITNTASGTNNDTYTVAFTPGTAPALPT
                     YTVTDTTLNTTTAPATYTAGTAISLGNGQTITFNGTPVAGDSYTVAPTPVAFNQNSSA
                     TYNYSTSTTVYDSLGGSQTVNMYFAKTSAGTWNVYAGTSTGTASLIGQANFNSSGTML
                     GTSQAVAPVPPSTTPTFTPTATPYAFNFQIPNNDGSSTPQNLTLNIGGTTQYGGKDGV
                     NSLNTDGYAAGTLTGFTINSDGTLTGNYSNQQTTTLGQIVLANFADPNGLVDIGGNNY
                     EQTSVSGVAQISAPGSTNHGVLQGGAVENSNVDLTSELVNLITAQRNYQANAQTIKTQ
                     QTVDQTLINL"
     gene            4758..5516
                     /gene="flgF"
                     /locus_tag="VHV99_15065"
     CDS             4758..5516
                     /gene="flgF"
                     /locus_tag="VHV99_15065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010112969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HEX3636777.1"
                     /translation="MDRLIYTAMSGSAQALEQQAIVANNLANASTTGFRAQLATFRAV
                     PLAFGDGSSINDDTTRTFVLSSTPGADYTPGPIQQTGNPLDVAIQGPGWLAVQTADGN
                     EAYTRAGNLHVDENGQLVNATNQVVLGNGGPVAVPPGAEITIGKDGTVSALTPGDPPT
                     AVVVVDQLKLVNPDPQTMTRGDDGLFRTADGNPADADPTVTLAPASLEGSNVNPVSAM
                     VAMITNARQFQMQTQLLQNADKNDQSANELLTFS"
     gene            5723..6511
                     /gene="flgG"
                     /locus_tag="VHV99_15070"
     CDS             5723..6511
                     /gene="flgG"
                     /locus_tag="VHV99_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HEX3636778.1"
                     /translation="MNRSLYIAATGMNAQQAQMDVISNNLANVSTNGFKGSRAVFEDL
                     LYQTIRQPGANSTQNTQLPSGIQLGTGVQQVATERLYTQGNLQQTGNSKDVAINGQGF
                     FQVQMPDGTTAYTRDGSFQTNAQGQLVTSSGYQVIPAITVPNNATSLTIGSDGVVSIT
                     VAGSTNTQQLGSMQIATFINPAGLDANGENLFSETASSGAPNVAQPGLNGAGTLEQGY
                     VEASNVNVVQELVNMIQTQRAYEINSKAVTTSDQMLQTLSQMQV"
     gene            6571..7269
                     /gene="flgH"
                     /locus_tag="VHV99_15075"
     CDS             6571..7269
                     /gene="flgH"
                     /locus_tag="VHV99_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HEX3636779.1"
                     /translation="MSHFTRNPVHSRTAQALVQLTLLAALGGCSLVPKQPITQQPMTA
                     LPPMPPQAQSPGSIYNPGYAGRPLFEDQRPRNVGDILTIVIQENVNATKSSGANANRT
                     GSTNFSVPTAGFLGGLFGKTNLSANGANVFAGTGGANASNTFNGTITVTVTGVLPNGN
                     LAVSGEKQMLINQGDEFVRFSGVVNPNTISNLNAVYSTQVADAKIEYSAKGYVNEAET
                     MGWLQRFFLNVSPW"
     gene            7272..>8061
                     /locus_tag="VHV99_15080"
     CDS             7272..>8061
                     /locus_tag="VHV99_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007746215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HEX3636780.1"
                     /translation="MRTVFFRLGRVAQLAQFGRLGRIGVVGRALAFVALACAALPAVT
                     PAHAERLKDLAQIQGVRDNPLIGYGLVVGLDGTGDQTTQTPFTTQTLANMLANLGISI
                     NNQAAGSTNQQSSLSNIQLKNVAAVMVTAVLPPFARPGEQIDITVSSLGNARSLRGGT
                     LLLTPLKGADGQVYALGQGNLAVGGAGASANGSRVQVNTLAAGRIAGGAIVERAVPTR
                     VSQAGTMQLDLNDMDYDTTQRVVAAVNNAFGSGTATALDGRTIQL"
BASE COUNT         1606 a         2690 c         2503 g         1262 t
ORIGIN      
        1 cccacaggcg cgcgccgctc ggcatgaacg gttcgagcgt cgtgcaggcc gcgagcccgc
       61 gcggaaatgc cggggcgacg gtgatctcga ccttgccggg caagcctgcc gactgctgct
      121 gcaagaaggc gagtgcggcg ctgcgaatcg cttcggcgtc ttcctgtccg gcgagcggta
      181 cgggttgcgc caccgccgcc gcgccggggc gcaggttctg ttgcgccacg gccggcgcca
      241 cttggcgcat cgacggcgtg cgggcgaggg tggcgggcgc ggccagcatc gatacgggcg
      301 tcggtgcccc tttcgctgcg gccgccgtgg tcacgatcgg cttgccgttc gcgcccaact
      361 gcggcggctc gccgcgcgaa gcaagactgt cgaagccacc ggctgcgggc gcgttgccga
      421 ttgcgctttc aacaccgctg ccgggcgcat tggcggccac cggctgcacg cccgtgcctt
      481 gccgtccggc attgagcgcg ggcgcgggcg tgaccacgac cggcacgact tgacgcgaac
      541 cctgcatcag attgacgcgt gcaaccgcgg cggcggaatt ggctgacgca gcattggcat
      601 ttcccggcgc gggaatcgtc acgacgccat tcgcatcggg cctgaagctg gtgcggatca
      661 tccgcggcgc ggcaggcggt tcgcccgcgc cgggaatgac aatcgagccg ctcgcattgg
      721 cggcgcgcgt gaatggatcg gcgtcgcgcg cgaccggccc cggcgtcagc gactgcgcgg
      781 tagcggcggc gagcgtcgcc gcggaccctg cgcgtttcgc gggcgttgcc tgcatctgct
      841 cggccagatc ggcgagcgcg gcgggattct tttcgcccgg tccgggaatc acgatcgggc
      901 cgccagcgtc ctgagcctgc gcggcggccg gcagcagcac gatgccgcct gcgacccaca
      961 gcgccacgtt gacgacgagg cgcaccagaa gacgggacgt gcggcgtaca gctatccgtg
     1021 acgtacgccg cacagcgtcc acgtccacgc ccacgcgacg cgccgtcgcc gcgcggctcg
     1081 cgcggtgcgt cagatcgagg gtgggctggt ccatcgtgtt tctccatcga atacatcggt
     1141 acgccttgca ttctagtgag cacccccatt gcgcaaacgt tgaatagaag ccccctttcc
     1201 cggttattcg tccgattgcc acttgcgttc ggcccataag atgcacctca tgcaaaaagg
     1261 tcttcccgtc cggcgcggtg ctcatacgag gcgcagcaag ggacaaacgg gaagactcgg
     1321 gaacgttctc cggagatttc aatgctggac aaactcgatg ccgaattcgc ctttggccgc
     1381 caggcgctcg atgtacgcgc ttaccggcag gaactgctgt cgtcgaatat cgccaatgcc
     1441 gatacccccg ggtacaaggc gcgcgacgtc gacttcgctt cgtcgctggc cggcgcgcta
     1501 aagaagacgg gcggcggtgc ggccacgggc gcctcgaaca attcgacgct cgccatgacg
     1561 cagcccgcgg gggtgacgag cggcatgtcg atggcgacga ccgcgccggg ccacatgtcc
     1621 ggcaacgcga cgctgacgcc gaccggcggc acgcccgacg actacggcaa tttgcaatac
     1681 cgcattccga cgcagcccgc gctcgacggc aacacggtcg acatcgacac ggagcgggtg
     1741 cagttcgcgg acaacacggt gcacttcgaa tcaggcatga cggtgctgtc gaaccagatc
     1801 aagacgatgc tttcggcgat cacgtcgggc tcgtaaataa ggcgcgcaaa gacgcacagc
     1861 aacaggcacc gcagaaaaga cacagcagta cgcacgaaca atcgctacgg gatttaactt
     1921 gaagccgggc cgagccgtgc aactacgttg catggctcgg agtaagcgct gaggagaggt
     1981 caggaaacat gccatcttta atgaacattt tcggtgttgc aggctccgcg atgtcggcgc
     2041 agtcgcagcg gctcaacgtg acggcgtcga atctcgccaa cgcggacagc acgaccggcc
     2101 cggacggcca accgtacagg gccaagcagg tcgtgttcgc ggtcagcccg ctcggcggcg
     2161 cgcgcacggg ctccggccag cagatcggcg gcgtgcaggt caccggcgtg gtcgacgacc
     2221 cgacgccgat gaagaccgcc tacgaccccg gcaatccggc ggccaatccg gacggttatg
     2281 tcacgttgcc caacgtcgac ccggtgcagg aaatggtcaa catgatctcg gcctcgcgct
     2341 cgtaccaggc caacgtcgag acgctgaaca ccgccaagtc actgatgctg aaaacgctca
     2401 cgatcggaac ctgaggagag ctccttgacc accaacacca ccatcggcaa caacggcacg
     2461 acgctgtcgc aaacgctcct cgatacgatg aacggcacga aaagtgccac gagttcgacg
     2521 agcagcgcgt ccagttcctc ggcgagcagt ctgcagagca cgtttctgca actgctcgtc
     2581 acgcagatgc agaaccagga cccgaccaat ccgatggaca gctcgcagat gacctcgcag
     2641 ttggcgcaga tcaatacggt ctcgggtatc agccaactga acacgacgct cacgtcgctt
     2701 gcctcgcagt tgtcatcgag ccaggagccg caggccgcga gcctgattgg cgcgaccgtg
     2761 ctcacccccg gcacctcggt gtcggttgcg agcggccagg ccggcgcgtt cggcgtgcaa
     2821 ctcgccaact cggtgagcga cctgcaagtg gtcgtgaaga actcgtcggg acagatcgtc
     2881 aacacgatcg acctcggccc gcagtcggcc ggcacggttc caatcggctg gacgccgacc
     2941 gatacgacag gcaccacgct gcccaatggt acctacacga tcagcgcggt cggcacgatc
     3001 aacggtcagc cggctacggc taccacgctc acgggctcca cggtggaaag cgtggtctcg
     3061 cagagcggtg gtacgccggg cctcgtgttg tcgaacggct cgacggtcgg cctgagcagc
     3121 gtcgccgcca tcatctgatt cagattctgc cagttacgac ttcccagaca cggagatcat
     3181 catgggttac gaacaaggtt taagtggttt gcaggcagcg tcgagcgacc tcgacgtgat
     3241 cggcaacaac attgccaact cgaataccgt tggcttcaag agcggctccg cgcagttcgc
     3301 cgatatgtat gccaattccg tggcgaccgc ggtgggcaat cagatcggta tcggcactca
     3361 gctcgccgac gtgcagcagc agttctcgca aggcacgatc accaccacca acgacgcgct
     3421 ggacgtagcg atcaacggca acggcttttt ccagatgtcg agcaacggct cgctggtgta
     3481 ctcgcgcaac ggcgtgtttc aactgaacaa caccggcggc atcaccaact cgcaaggcct
     3541 gcaactaatg ggctatgccg cgaacagctc gggtgtcatc aacaccgcgc agaccgtgcc
     3601 gctcaccgtg ccgaccgcga acatcgcgcc gcaacctagc accacgatca ccgcgggcct
     3661 gaatctgaac gcgcaggatc cgctgatgct cggccagcca aacgtgacct ccgcactcgg
     3721 ggccggcagc gggctgacga cgcccggcgc gaccattacg aacaccgctt cgggtacgaa
     3781 caacgacacc tacaccgtcg cgttcacccc gggcaccgcg cccgctctgc ctacctacac
     3841 ggtgacggac accacgctta acacgacgac cgcgccggca acctatacgg ccggcacggc
     3901 aatctcgcta ggcaacggcc agacaatcac gttcaatggc acgccggtcg ccggcgacag
     3961 ctacacggtc gcgccgaccc cggtggcgtt caaccagaac agctcggcga cctacaacta
     4021 ctcgaccagc acgacggtct acgattcgct cggcggatcg cagacggtca acatgtactt
     4081 cgccaagacg tcggccggta cgtggaatgt gtacgccggc acgtcgacgg gcacggcgag
     4141 cctgattggt caggccaatt tcaattcgtc gggcacgatg ctcggcacga gccaggcggt
     4201 cgctccggtt cctccctcga ctaccccgac tttcaccccg accgccacgc cgtacgcctt
     4261 caacttccag atcccgaaca acgacggttc ctcgacgccg cagaatctga cgctgaacat
     4321 cggcggcacg acgcagtacg gcggcaagga cggcgtgaac tcgctgaaca ctgacggcta
     4381 tgccgccggc acgctgacgg gcttcacgat caactccgac ggcacgctga ccggcaacta
     4441 ttcgaaccag caaaccacga cgcttggcca gatcgtgctg gcgaacttcg cggacccgaa
     4501 tggtctcgtg gacattggcg gcaacaacta cgagcaaacg tcggtttcag gcgtcgcgca
     4561 gatttcagcg ccgggctcga ccaaccatgg cgtgctgcaa ggcggcgcgg tggaaaactc
     4621 gaacgtcgat ctgacgagcg agctggtgaa cctgattacc gcgcaacgca actatcaagc
     4681 gaacgcgcag acgatcaaga cccagcagac cgtcgaccag accctgatca acctgtaagc
     4741 gagcgggact ctccaccatg gatcggctga tctacaccgc gatgtcgggc agcgcacaag
     4801 cgctcgaaca gcaggctatc gtcgccaaca acctggcgaa cgcctcgacc accggctttc
     4861 gcgcacaact tgcgaccttc cgcgccgtgc cgctggcgtt cggcgacggc agttcgatca
     4921 acgacgacac cacgcgcacc ttcgtgctgt cgtcgacgcc gggcgccgac tatacgccgg
     4981 ggccgatcca gcagacgggc aatccgcttg acgtggcgat tcagggtccg ggctggctgg
     5041 cggtgcaaac cgccgacggc aacgaggctt acacacgcgc cggcaatctg cacgtcgacg
     5101 agaacgggca actggtgaac gccaccaatc aggtggtgct cggcaacggc ggtccggtcg
     5161 cggtgccgcc gggtgcggaa atcaccatcg gcaaggacgg caccgtgtcc gcgctgacgc
     5221 ccggcgatcc gcccaccgcg gtggtggtgg tcgatcagct gaagctggtg aatccggatc
     5281 cgcaaaccat gacgcgcggc gacgacggcc tctttcgaac cgccgacggc aaccctgccg
     5341 atgccgatcc gaccgtcacg ctggcgccgg cttcgctcga aggcagcaac gtgaatccgg
     5401 tgagtgcgat ggtcgcgatg atcaccaacg cccgccaatt ccagatgcag acccagctgc
     5461 tgcaaaacgc ggacaagaac gatcagtccg ccaacgagtt gctgactttt agctaacgga
     5521 ttgcgcgggg ttgccgtgac atggcaacca tgttgcgtga cagctttgga tgggatcgga
     5581 gtaagcgctg aagcgcccac tccggtcgac cgctttgcat gggaccggaa taagcactga
     5641 agcgctaact ctggtcgacc gctttgcatg ggaccagagt aagcgctgaa gcgctaactc
     5701 tggtcgacat aggagaagaa ttgtgaatcg ctcgctctac atcgccgcta ccggcatgaa
     5761 tgcgcaacag gcgcagatgg acgtgatttc gaacaacctc gcgaacgtca gcaccaacgg
     5821 cttcaaaggc tcgcgcgcgg tgttcgagga tctgctgtat cagacgatcc gccagccggg
     5881 tgccaactcg acgcaaaaca cccaattgcc gtccggcatc cagctcggca ccggtgtcca
     5941 acaggtcgcc accgagcgtc tatacacgca gggcaacctg cagcagaccg gcaactcgaa
     6001 agacgtcgcc atcaacggtc agggcttttt ccaggtgcag atgccggacg gcacgaccgc
     6061 ttacacgcgt gacggttcgt tccagaccaa cgcccaaggc cagctcgtca cgtcgagcgg
     6121 ctatcaggtg attccggcga tcacggtccc gaacaacgcg acctcgctga ccatcggcag
     6181 cgacggcgtg gtgtcgatca ccgttgctgg ttcgaccaac acccagcagc tcggctcgat
     6241 gcagatcgcc acctttatca acccggccgg cctggatgcg aatggtgaaa acctgttctc
     6301 ggaaaccgcc tcgtcgggcg cgccgaacgt cgcgcaaccg ggtctgaatg gcgccggcac
     6361 gctcgagcag ggttacgtgg aagcgtcgaa cgtgaacgtt gtgcaggaac tggtgaacat
     6421 gatccagacg cagcgcgcct acgaaatcaa cagcaaggcc gtgacaacgt cggaccagat
     6481 gctgcagacc ctcagccaga tgcaggttta aggcttgagg gactgggaat caagagcgcc
     6541 ccggcgttct ctcgaaagac ggtaaccaag atgtcgcact tcactcgcaa tccggttcat
     6601 tcgcgcaccg ctcaagcgct cgtgcagctc acgctgctcg cggccctggg tggctgtagc
     6661 ctcgtgccga agcagccgat cacgcagcag ccgatgacag cgctgccgcc gatgccgccg
     6721 caagcacagt cgccgggctc gatctacaac ccgggttacg cgggccggcc gctgttcgaa
     6781 gaccagcggc cgcgcaatgt gggcgacatc ctgacaatcg tgattcagga aaacgtcaac
     6841 gcgacgaagt cgtcgggcgc caacgcgaat cgcaccggta gcaccaactt cagcgtgccg
     6901 acggcgggct tcctgggtgg actgttcggc aaaaccaacc tcagcgccaa cggcgcgaac
     6961 gtgtttgccg gaaccggcgg cgccaatgcg tcgaacacgt tcaacggcac gatcaccgtg
     7021 acggtgaccg gtgtgctgcc gaacggcaac ctggcggtga gcggcgaaaa gcagatgctg
     7081 atcaaccagg gcgacgaatt cgtgcgcttc tccggcgtgg tgaatccaaa cacgatctcc
     7141 aacctgaacg ccgtctactc gacccaggtg gccgacgcga agatcgaata ctccgcgaag
     7201 ggctacgtca acgaggccga gaccatgggc tggttgcagc ggttcttcct caacgtgtcg
     7261 ccgtggtgat catgcgtacc gtcttcttcc gccttggccg cgtcgcgcaa ctcgcccagt
     7321 tcggccggct tggccgcatt ggcgttgtcg gccgcgccct cgctttcgtc gcgctcgctt
     7381 gcgccgcgct gccggccgta acgcccgccc atgccgagcg tctgaaggat ctcgcgcaaa
     7441 ttcaaggcgt gcgtgacaac ccgttgatcg gctacggtct cgtcgtcggt ctcgacggca
     7501 cgggcgacca gaccacgcag acgccgttca ccacgcagac actcgccaac atgctggcga
     7561 acctcggcat ctcgatcaac aaccaggcgg cgggctcgac caaccagcag tcgtcgctgt
     7621 cgaacatcca gttgaaaaac gtcgccgcgg tgatggtgac ggcggtgctg ccgccgttcg
     7681 cgcgtcctgg cgaacagatc gacattaccg tgtcttcgct cggcaatgcc aggagtctgc
     7741 gcggcggcac gctgctgctc acgccgctca agggcgccga tggccaggtg tacgcgctcg
     7801 gccagggcaa cctcgcggtc ggcggcgccg gcgcgagcgc gaacggcagc cgggtgcagg
     7861 tgaatacgct ggctgccggc cgcatcgccg gcggcgccat cgtcgaacgc gcagtgccga
     7921 ccagggtatc ccaggcgggc acgatgcagc tcgatctgaa cgacatggac tacgacacca
     7981 cgcagcgcgt ggtggccgcg gtcaacaacg cgttcggcag cggcacggcc accgcgctcg
     8041 acggccgcac gatccagttg c
//
LOCUS       DATASH010000544         1581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1275924,
            whole genome shotgun sequence.
ACCESSION   DATASH010000544 DATASH010000000
VERSION     DATASH010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1275924"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VHV99_15085"
     CDS             <1..292
                     /locus_tag="VHV99_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX3636781.1"
                     /translation="DDVDQALAALRDLAVADPRALQTPAPQVMVARFDDSTVVLNIRV
                     WANIDTFWDMRWDLARQVRQTLNDAQCGLPVRTRELHIVQSNETDESVAARV"
     gene            complement(422..976)
                     /locus_tag="VHV99_15090"
     CDS             complement(422..976)
                     /locus_tag="VHV99_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycolipid-binding domain-containing
                     protein"
                     /protein_id="HEX3636782.1"
                     /translation="MRELRWASQEGDGFEHLAFEAREDGFAVESVLVGQRYGKAYGLH
                     YKVRCDAQWRTTYAWLKIVGGAELELHGNGAGHWHDGHGLVLSAIEGCIDIDIAATPY
                     TNTLPIRRLQLAGGERQAISVAYISTPDLQVTRAEQAYSCIELNRVYRYEGIFRNFTA
                     DLTVDEDGLVIDYPTLFTRLPRER"
     gene            1230..>1581
                     /locus_tag="VHV99_15095"
     CDS             1230..>1581
                     /locus_tag="VHV99_15095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007743693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015204 - urea transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071918 - urea transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HEX3636783.1"
                     /translation="MHAAATEAPSAALRTLLRSVGQIVLQSNAFAGACLLAAWLLYDP
                     RLACAALVGAVAANVSAVLAGYREDDTRAGLHGFNGALAGLAAFSFIADNATAAAVAI
                     LAATGTAWLLEPWSR"
BASE COUNT          280 a          531 c          489 g          281 t
ORIGIN      
        1 tgacgacgtc gaccaggcgc tggccgcact gcgcgatctc gccgtcgccg acccgcgcgc
       61 gttgcagacg cccgcgccgc aggtgatggt ggcccgtttc gacgacagca ccgtggtcct
      121 gaacatccgc gtgtgggcca atatcgacac gttctgggac atgcgctggg acctcgcccg
      181 gcaggtccgt caaacgctca acgacgcgca atgcggcttg ccggtgcgta cacgtgaact
      241 gcatattgtg caaagcaacg agaccgacga atccgtcgcg gctcgcgtat agccgcactg
      301 aaatcgacgc cgggcattga gtcgcgcgaa cccgagcgta ttgcggcagg catcgaacgc
      361 tttgtcgagc agcaccggga acagccgccg gcagacagcg gcggttccgg cacctcgggg
      421 ttcaacgctc gcgcggcagg cgcgtgaata gcgtcggata gtcgatcacg aggccgtctt
      481 catccaccgt caggtccgcg gtgaaattgc ggaagatgcc ctcgtagcga tacacgcgat
      541 ttaactcgat gcacgaatac gcctgctcgg cacgcgtaac ctgcaaatcc ggcgttgaaa
      601 tataggccac ggaaatcgct tggcgctcgc ctccggcgag ttgcaaacgg cggatcggca
      661 acgtgttggt atacggcgtg gcggcgatat cgatatcgat gcacccttcg atcgcactca
      721 gcacgaggcc atggccatcg tgccaatgac cggcgccgtt gccgtgcaat tccagttccg
      781 ccccgccgac gatcttcagc cacgcgtagg tggtgcgcca ttgcgcatcg cagcggacct
      841 tgtagtgcag gccataggcc ttgccgtagc gctgcccgac aaggacgctc tcaaccgcga
      901 agccgtcctc gcgcgcctcg aacgccagat gttcgaaacc gtcaccctct tgcgatgccc
      961 agcgtagttc acgcatcaac ctgtctcctt gtttgaacgg cacggcacgc gcgccgggtt
     1021 catatcgtag cgcgaaaact ttcggttcgc cgacagatgg ccgtggcgcg cagatgtcgc
     1081 aaatagccac tatcaacgcc tcgcgatgcc cggcgtggtg cgagtggcct atgctcgcgg
     1141 ttcacactca acctgaaacc tgacccgccg cgtgcgcctg attcgcatgc ggcaatgtcg
     1201 cgtcacatcg aattcagcgc cttaaagcca tgcacgccgc cgcaaccgaa gcaccatccg
     1261 ccgccttgcg caccttgctg cgcagtgtcg gccagatcgt gctgcagtcg aacgcgtttg
     1321 ccggcgcgtg cctgctcgcc gcgtggctgc tatacgatcc acgcctcgcc tgcgcggcgc
     1381 tggtgggcgc ggtcgcggcc aacgtgagcg cggtcttggc cggctatcgc gaggacgaca
     1441 cgcgcgctgg gctgcacggc tttaacggcg cgctcgccgg tctcgccgcg ttcagcttca
     1501 tcgccgacaa tgcgacggcc gctgccgtgg cgattctggc cgcgacaggc acggcatggc
     1561 tgcttgagcc atggtcgcgc c
//
LOCUS       DATASH010000545         4195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1276579,
            whole genome shotgun sequence.
ACCESSION   DATASH010000545 DATASH010000000
VERSION     DATASH010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1276579"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VHV99_15100"
     CDS             complement(<1..197)
                     /locus_tag="VHV99_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HEX3636784.1"
                     /translation="MFRNTARCLDALFVIAGGLIAHWMRFSPPIALSDTERLLIAFNC
                     VLVLLMFPGFGVYETWRGKALP"
     gene            complement(233..967)
                     /locus_tag="VHV99_15105"
     CDS             complement(233..967)
                     /locus_tag="VHV99_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX3636785.1"
                     /translation="MLTLQSDLHGNHLLGALPSHEWQALAPHLELVHLRTEQLLCDSG
                     QRIHHVYFPTTAIISMLSTMEDGSSVEIAAVGREGMTGVPVLTGGETMPNRVQVQCAG
                     FAYRMSAQALKQQFARSDFLRRLMLLYMHALLTQVAQTAACNRHHALNKQLCRWLLIE
                     VDRVASNDLTVTQQLIADMLGVRREGITEAAGKLHDEGLIHHSRGHIRILDRKGLEAR
                     ACECYGLVKREFDRLLPRLRQAETVE"
     gene            complement(1600..4104)
                     /locus_tag="VHV99_15110"
     CDS             complement(1600..4104)
                     /locus_tag="VHV99_15110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HEX3636786.1"
                     /translation="MYQDLWPQRLDAGWQCVSTPAGACASPADLPAEGWLPAQVPGTV
                     ASARRAAGLFDVANPPPLAFDDHWYRLTLTGTGKRRLRLHGLATLAEVWLDGIKRLES
                     DSMFVAHDLDIELQGSATLALCFRSLTPALAAKRSRARWRPRLVTPSTLRNVRTTLLG
                     HMPGWCPSVQAVGPWRPVELLSDAPHAFDTIDLTSRLEDDDGIVSLTLHFVHPHDVDR
                     CAASLNCDGIVSSLQWRDAYTLTGNVRVPHAERWWPHTHGAPTLYPLTLQLGDDSAIS
                     HSLGSVGFRSIEVDRGADGAGFALRVNGTPVFCRGACWTSADLVTLSGTEAQLRHAFA
                     LARDAGMNMLRVGGTMVYESDAFYALADEYGLLVWQDFALANFDYPNDAVFSAAVERE
                     ATQFLTRTRRFASLAVLCGGSEVDQQAAMFGLPASMRAQPLFTELLPAIATRERPDVA
                     YVSNSPSGGAWPFSTNEGITHYYGVGAYQRPLDDVRRAQVRFAAECLAFANVPDDATL
                     HDAPGTIHSHDPRWKAAVPRDPGAGWDFDDVRDHYLQTLYGIEPARLRYEDPERYLDL
                     SRAVVAELMGDVFAEWRRAGSTCGGALVWQLQDLRPGAGWGLIDATGRPKSALHGLAA
                     TLQPIQLVMTDEGLNGLDIHLLNERAEPLHAQLDLVCLREGSVKVASASRAVDLAPHS
                     TQRIGAAECLGQFFDFTHAYRFGPRAHDVTIATLRDPASGRIVSEAVHLPERRAGERH
                     DLGLTVGTERTAGGWQLVIEAKRFARFVHIIDAHYRAAHDWFHLPPNRPCIVPLIAVE
                     KLVAHASNAAFKADATCATPAGEVRAINALSATFYG"
BASE COUNT          790 a         1226 c         1416 g          763 t
ORIGIN      
        1 ggcagcgcct tgccgcgcca cgtttcatag acgccgaagc cgggaaacat caacaacacg
       61 agcacgcagt taaacgcaat cagaaggcgc tcggtatccg acaacgcgat aggcggcgaa
      121 aaacgcatcc agtgagcgat caggcctccc gcaatcacga agagtgcgtc gaggcatcgc
      181 gcagtattcc tgaacatatg cgggtctacc tcttagtcga gcggtaccat gcttattcga
      241 cggtttcagc ctggcgcaga cgcggcagaa gacggtcgaa ttcgcgcttg acgaggccgt
      301 agcattcgca ggcgcgcgct tcgagacctt tgcgatcgag gatcctgatg tggccgcggc
      361 tgtgatgaat caggccttcg tcgtgcaact tgccggccgc ttcggtaatg ccttcgcggc
      421 gcacgccgag catgtcggcg atcagttgct gggtaaccgt cagatcgttc gaggccacgc
      481 gatccacttc gatcagcaac cagcggcaca gttgcttgtt caacgcgtgg tggcgattgc
      541 aggcggcggt ttgcgcgact tgcgtgagca gggcgtgcat gtacagcagc atcaggcggc
      601 gcaggaaatc agagcgtgcg aattgctgct tgagcgcctg tgcgctcatc cggtaggcga
      661 agccagcgca ttgcacctgc acgcggttcg gcatggtttc gccgcccgtg agcaccggca
      721 cgccggtcat gccttcgcgg ccgaccgcgg cgatttcgac cgagctgccg tcttccatcg
      781 tcgagagcat cgagatgatc gcagtggtcg ggaagtagac gtgatggatc cgttgacctg
      841 aatcgcacag cagttgttcg gtgcgcagat gaaccagttc gagatggggg gcaagtgctt
      901 gccattcgtg ggacggcaat gcgccgagca aatggttgcc gtgcaaatct gattgaagtg
      961 tcaacatgat cggtcctcgc tgaagccgcg cgttgcgtgg cttcgtgttt ttgatccgat
     1021 gcctgcatcc ttgaattaac aactttgctg cctggcgagc agcgagggat gaacaggaac
     1081 ggccccgtat tggcctgatg caccaccgcc gttcttgtag atctcgttgc ggcgacgcga
     1141 tgttgcgctt tctctttagc gaggagcgtg ccagccatag ggaaaacgag tgctggtgcg
     1201 gcgcagcgaa ccaggcctta gcggcgcgcg aagccaaacg tggctttttg tttcaattct
     1261 gcacaacgcc tggttagcgc agcacccgtt cgaaggatgt ttttattcga gccaagtcat
     1321 tgatcggatt ggcttagcgg gtgatgagca gcgctttgcg gtggctgtct cacggatgcg
     1381 atagccgttg tgggggcgtt atcgggggtc gtccgggatg cgttggaatt gattcagatg
     1441 tgttctatac gctgcgtgtg cttgcgtacg gagttccaac tttgaagagc gggaaagtcg
     1501 caaaagaacc aacactgatt ccgcagacca gacaggtggc ttcgcgcagc gcgaggtttt
     1561 atgcgggttg cgaagggacg gctagttcag ggaagacaat cagccgtaga aggtggcgga
     1621 tagcgcattg atcgcgcgga cttcgcctgc gggcgtcgcg catgttgcgt ctgctttaaa
     1681 cgccgcgtta gatgcgtgtg caacgagttt ctcaacggcg atgagcggca cgatgcaggg
     1741 gcggttcggc ggcagatgga accagtcgtg cgcggcgcgg taatgcgcgt caatgatatg
     1801 aacgaagcgc gcgaagcgct ttgcttcgat gaccagctgc cagccgccgg cggtgcgctc
     1861 ggtgccgacc gtcaggccga gatcgtgacg ctcgcccgcg cggcgctccg gcagatgaac
     1921 ggcttctgaa acgatccggc cgctcgccgg atcgcgcagt gtcgcaatcg tgacgtcgtg
     1981 ggcgcgcggg ccgaagcggt aggcgtgggt gaaatcgaag aactggccga ggcactcggc
     2041 cgcgccgata cgctgtgtgc tgtgcggcgc caggtcgacc gcgcggctgg ccgacgccac
     2101 cttgacgctg ccctcgcgca ggcagaccag atccagttgc gcgtgcaacg gctcagcccg
     2161 ttcgttcagc agatgaatat cgaggccgtt gaggccttcg tcggtcatga ccagctggat
     2221 gggttggagc gtcgccgcga gcccgtgcag ggcgctttta ggccgtcctg tggcatcaat
     2281 caggccccag cccgcgccgg ggcgcagatc ctgaagctgc cagaccagcg cgccgccgca
     2341 ggtcgagccg gcgcgccgcc attcggcgaa gacgtcgccc atcagctcgg cgaccaccgc
     2401 ccgcgagaga tccagatagc gctcagggtc ttcgtaacgc aagcgtgccg gctcgatgcc
     2461 gtagagcgtc tgcagataat ggtcgcgtac gtcgtcgaaa tcccagccgg cgccggggtc
     2521 gcgcgggacc gcggctttcc agcgcggatc gtgcgaatgg atcgtgcctg gcgcgtcgtg
     2581 caaggtggcg tcatcgggca cgttggcgaa cgccagacat tcggcggcaa aacggacctg
     2641 tgcgcggcgc acgtcgtcga gcgggcgttg ataggcgccc acgccgtagt aatgcgtgat
     2701 gccctcgtta gtcgaaaacg gccacgcgcc gcccgagggc gaattgctca catacgcgac
     2761 gtccggccgt tcgcgcgttg caatggccgg taacagttca gtaaaaagcg gttgcgcgcg
     2821 catcgaagcg ggcaggccga acatggccgc ctgctggtcg acttcgctac cgccgcacag
     2881 caccgcgagc gatgcgaagc gccgcgtgcg ggtcagaaat tgtgtggctt cgcgctcgac
     2941 ggcggcgctg aagacggcat cgttcgggta gtcgaaattc gccaacgcga agtcctgcca
     3001 gacgagcagg ccgtattcgt cagcaagcgc gtagaacgcg tcggactcgt acaccatcgt
     3061 gccgcctacc cgcagcatgt tcatgcctgc atcgcgggcc agcgcgaaag cgtggcgcaa
     3121 ttgggcctcg gtgccgctca gcgtgacgag atccgcgctg gtccagcagg cgccgcggca
     3181 gaacaccggg gtgccattta cgcgcaatgc gaagcccgcg ccgtccgcgc cgcgatccac
     3241 ttcgatgctg cgaaagccga ccgatcctaa tgaatgagaa atcgcggagt cgtcaccaag
     3301 ttgcaacgtc agaggatata aagtgggcgc gccatgcgtg tgcggccacc atcgttcggc
     3361 gtgcggcaca cgcacgttgc cggtgagtgt atatgcatcg cgccattgca aagacgaaac
     3421 aatgccatcg caattcaacg aggcagcgca tctgtcgacg tcatgcggat gcacgaaatg
     3481 tagcgtgagc gaaacaatgc cgtcgtcgtc ttcaagacgg cttgtcaggt cgatcgtgtc
     3541 gaaggcgtgc ggcgcgtcgc ttaacagttc gaccggacgc cacgggccga ctgcctgcac
     3601 cgagggacac cagccgggca tatggccgag cagggtggtg cgcacgttgc gcaaggtgga
     3661 cggtgtaacc agccgaggcc gccagcgggc gcgtgagcgt tttgccgcca gcgcgggtgt
     3721 cagcgagcga aagcacagcg cgagcgttgc gctgccctgc agttcgatgt cgagatcgtg
     3781 cgcgacgaac atcgaatcgg actccagacg cttgatgccg tcgagccaga cttcagcgag
     3841 cgtggcgagg ccgtgcaggc gcaagcggcg tttgcccgtg cctgtcagcg tgagccggta
     3901 ccagtgatcg tcgaaggcga ggggaggtgg attcgcaacg tcaaagagac ccgcggcacg
     3961 gcgcgcgctt gccacggtgc cggggacttg cgcggggagc cagccttcag caggcaagtc
     4021 ggcgggtgag gcgcatgcac cggccggcgt gctcacgcac tgccagccgg catcgagccg
     4081 ttgtggccac aggtcttgat agatggcctt gggtgaggca gcggccggaa gcgggccgcc
     4141 ttcatccgat accagctcag tcgtcagcgc atccacggcg ttttcctgaa gctcg
//
LOCUS       DATASH010000546         6619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1276692,
            whole genome shotgun sequence.
ACCESSION   DATASH010000546 DATASH010000000
VERSION     DATASH010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6619
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1276692"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="VHV99_15115"
     CDS             <1..957
                     /locus_tag="VHV99_15115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3636787.1"
                     /translation="SIGLLGCGASGVLLLIAVLCGHWPVLSLALLVCSRLVLGFGESL
                     CGTGAILWGIGRVGTSNNARVISWNGIATYGALAIGAPLGVAITHSVGFATVGILVIL
                     LAALGFYLARPIAPVPIVHGERMSYRSVFTRVLPHGIGLALGSAGFGSIATFITLFYA
                     ARHWPNAALSLTVFGTLFIGARLLFANTIKAYGGFRVAIASFSLECAGLLMLWLAPEP
                     HIALAGAALTGFGFALVFPALGVEAVGLVPPASRGAALSAYSVFLDLSLGITGPLAGY
                     IAGEFGYAQVFLFAAVASALAVVLSTMMYLRNARAPNAPAAT"
     gene            complement(1927..3159)
                     /locus_tag="VHV99_15120"
     CDS             complement(1927..3159)
                     /locus_tag="VHV99_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEX3636788.1"
                     /translation="MPTSSTPSASAVSTAVPCPVVESLDAILPEEPLLMMGAGPVPIP
                     AAVAKANTVVINHLGSTMVKVIGQVKTMARYVFQTNSKWVLGVAGPGSAAMEMAISNL
                     AWEGTRVLSIKNGFFSERMAEMGRRVGARVSTLDVEDGTVASLDVVAEAIRRERPEVV
                     TIVQGETSNTVWNHQLREIAALAKAAGALVIVDAVCTLSTMPLEMDAWGIDAVITGGQ
                     KGLSSIPGVSLIAFSDEAWARVKGRTAPNAHWCLDASLAENFWHNAGYHYTAPVSGVL
                     ALHEALRLVCAETLEKRFARHLKCSVALQAGITALGLQLYAPAPCRLNSVVGIVVPER
                     LSSADICGHISRHHQVEISGSFGLPIVRIGQMGEQCREHNLFRTLHALGRTMVDLGVK
                     VELPAGVAALERWLSEAR"
     gene            complement(3273..4937)
                     /locus_tag="VHV99_15125"
     CDS             complement(3273..4937)
                     /locus_tag="VHV99_15125"
                     /EC_number="2.6.1.1"
                     /EC_number="4.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018431835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate transaminase/aspartate
                     4-decarboxylase"
                     /protein_id="HEX3636789.1"
                     /translation="MAKEKGGKKQGDMAQAGFEALSPFELKDELIKAAGGGAVERPAN
                     ASMLNAGRGNPNFLATIPRHGFWQLGLFAMRESERSFAFMPEGVGGFPRRDGLTERFD
                     LFLRENKGVPGIDFLRGAISYVRDQLGLSAGDFLYEMCEGILASNYPVPDRMLKLSEI
                     IVGQYLRKEMIGNHPFVGEFDVFAVEGGTAAMTYIFNTMRENHLIKAGDTIALGLPIF
                     TPYIEIPRLNDYQLNVVNLEATVESGWQYSKKELDKLRDPKVKAFFLVNPSNPPSVKI
                     NDESLQYIADIVKERPDLILLTDDVYGTFADDFVSLFALAPKNTILVYSYSKYFGATG
                     WRLGTIATHRDNVLDKLISELPKEEKKQLHERYESITTEPDKLKFIDRLVADSRTVAL
                     NHTAGLSTPQQVQMVLFSLFSLMDTPDAYKNALKRLIRKRKQALYEEVGITFDDDDPN
                     QVDYYTIVDIEFLGERMFGREFVDWLLKNAEPSELLFRLAREARVVLLPGRGFGTQHP
                     SGRVSLANLNESDYRQIGRAMRKLIEDYVERYNAATGKKLDKTKVM"
     gene            complement(5069..>6619)
                     /gene="aspT"
                     /locus_tag="VHV99_15130"
     CDS             complement(5069..>6619)
                     /gene="aspT"
                     /locus_tag="VHV99_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0070906 - aspartate:alanine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-alanine antiporter"
                     /protein_id="HEX3636790.1"
                     /translation="AAVVISQAGVTIDNGVKSVMFAVFIYAVGYDSGPGFFNSLNRKT
                     LREIAMAIFLAVTALITVVICAKLFHLNKGLAAGLAGGALTQSAIIGTAGDAIARLGL
                     PVDEVKSLQSDVAIAYAVTYVFGSLGAIIVCVNILPKFMGQSLRDASIEAERELSSGA
                     PALGAGQIRALPELVGRAYKIDVSAGKTVKAVETQHQDMLTIERIRRDGKALDPMPDL
                     TLQLNDEVLVVGRREAVVAFGSNGNEIANVEDIGVVMQTRDGVFTRKGMNHTTIAAAR
                     EMVDRDLRHGVYIQSASRAGQPLPVLPETKLEHGDVITFYGSPKDTKRAVEAAGYELP
                     YSNKTDFIYMGVGIVLGLLIGLIVVDVGGVPLTLGSGGGCLLAGLLFGWMRGKHPMYG
                     AMPSAASQLLKDFGLAAFVAVVGLNSGLQAVVTVKQSGVTIFLLGVVVTVLPLVLTML
                     FGRYVLRYNNAAILAGALTGSRSANPAFGGVLDKAESAVPTVPFAITYAIANVALTLL
                     GPLVVGLV"
BASE COUNT         1279 a         2074 c         1930 g         1336 t
ORIGIN      
        1 tcgatcggtc tcctgggttg cggcgcgagc ggcgtattgc tgctgatcgc cgtgttgtgc
       61 ggacactggc cggtgctgag cctcgcgttg ctggtgtgca gccgcctcgt gctcggcttc
      121 ggcgaaagcc tgtgcggcac cggcgcgatt ctgtggggca tcggccgggt cggcacaagc
      181 aacaatgcgc gggtgatttc gtggaacggc atcgcgactt atggcgcgct cgcgattggc
      241 gcgccgctcg gcgtagcgat tacgcactca gtcgggttcg ccacggtggg cattctggtg
      301 attttgctgg ctgcgcttgg attttatctg gctcggccaa tagcgccggt gccgatcgtg
      361 catggcgagc ggatgtcgta ccgcagcgtg tttacgcggg tgttgccgca tgggattggg
      421 ttggcgcttg ggtcggctgg gtttgggtcg attgcgacgt ttattacgct gttttatgcg
      481 gcgcggcatt ggcctaatgc ggctttgtcg ttgactgttt tcggcacgct gtttattggc
      541 gcgcggttgt tatttgccaa tacgattaag gcttatggtg ggtttcgggt cgcgattgcg
      601 tcattttcgc ttgaatgtgc agggttgttg atgctgtggc tggcgcctga gccgcatatt
      661 gctcttgcgg gggcggcgtt gaccgggttt gggtttgctc ttgtgttccc ggctctcggg
      721 gttgaggccg ttgggcttgt gccgcctgct agccgtggcg cggctttgtc ggcttattcc
      781 gtgttcctcg atctgtcgct ggggattact gggccactgg ccgggtatat cgctggggag
      841 ttcggatatg cgcaggtgtt tctttttgca gcagtggctt ccgcgctggc agtcgtgttg
      901 tcgaccatga tgtatttgcg caatgcgcgg gcgccgaatg cgcctgcggc gacttgacct
      961 tttttgcctg ctcggcgcag tttgtctgca cgcctgcgaa gttgggcttt gcttgagttg
     1021 gttgcctgcg cgttgctttg ttccgctgtt tgcctacggc gttggccttt ccttgagctg
     1081 gttgcctgcg cggcgttttt tggttgtatg cctacggcgt tggcctttcc ttgaattgat
     1141 attggattat tagcgttgcc cctgtgcggg gcggcaccta cttttctttg cctgccgcaa
     1201 agaaaagtag gcaaaagaaa gcggctcaca ccgccagctc ataagcgggt cccccgcgca
     1261 gttgcggtag tggtgcatct ggaatctgtg ttctcgcaca ttcggcgtaa gtgacacagc
     1321 cgtcatactt ccggccagcg ctgcgcgcgc cgaaaccctc ttcacgaaac cgcccgctgg
     1381 ttttcaccct cagttccacc aagtaagtct tgtcacccgt cattgcatca ccactgcggc
     1441 tttggctctg ggtgcggctt cgaatacgag tagcagcgcg gacaggaatt ggacacgtcg
     1501 gcatttcatt cgaaacaccg gttcgccagc gaagcgcatg gccccaccac gcccaaacga
     1561 agccctggtt tcccaggcag acccatccgc gacgcacgca gtgcggagtg ggcgctgaag
     1621 agcgcgttgt cactgacgtc gaatgtgcga cggcacggat tccagatgca ccactaccgt
     1681 aactgtgcgg gagacccact tatgagctag cggggtgagc cgctttcttt tgcctacttt
     1741 tctttgcggc aggcaaagaa aagtaggtgc ccgccccgca caggggcgac gctaatagac
     1801 caataagaaa acaaggaaag gccaacgcca taaaagcaca gacaaacaag cgcccgcagg
     1861 caaccagatc aagcaaaccc caaccccaca ggcatacaga caccaagcgc ccagcaggca
     1921 aaaacctcac ctcgcttccg acaaccaccg ctcaagcgcc gccacgccgg caggcaactc
     1981 aaccttaacc cccagatcca ccatcgtccg cccaagcgca tgcaaggttc gaaacaggtt
     2041 gtgctcccgg cactgctcac ccatctgtcc aatccgaacg atcggcaaac caaacgaccc
     2101 cgagatttcc acttgatgat gccgcgagat atgcccacag atatcagcag aactcagccg
     2161 ctccggcacg acaataccga ccaccgaatt cagccggcaa ggcgccggcg catagagctg
     2221 caagcccagc gcagtaatcc ccgcctgaag cgccaccgag cacttcaaat ggcgtgcaaa
     2281 gcgcttctcc agcgtctcgg cacaaaccaa ccgcaaagcc tcgtgcaagg ccaacacacc
     2341 cgacaccggc gccgtgtagt gatagcccgc gttatgccag aaattttccg ccagcgacgc
     2401 atcgaggcac caatgcgcat tcggcgccgt gcggcccttc acgcgcgccc acgcctcatc
     2461 cgaaaacgcg atcagcgaga cacccgggat tgaagacaag cccttctgcc cacccgtaat
     2521 caccgcatcg ataccccacg cgtccatctc cagcggcatc gtgctcagcg tacacaccgc
     2581 gtcgacaata accagcgcgc cggccgcttt cgcgagcgcg gcgatctccc tcaattgatg
     2641 gttccacacg gtattggacg tctcaccctg cacgatcgtc acaacctccg gacgctcgcg
     2701 gcgaatcgct tcagccacca catcgaggct cgcaaccgta ccgtcttcga catccagcgt
     2761 actcacccgc gcacccacgc gccggcccat ctccgccatc cgttcgctga aaaagccgtt
     2821 cttgatgctc agcacccgcg tgccttccca cgcgagattg gagatcgcca tttccatcgc
     2881 ggccgaaccc ggaccggcca cgcccagcac ccacttcgaa ttcgtctgga acacgtagcg
     2941 cgccatggtc ttcacctggc cgatcacctt caccatcgtg ctgccgaggt gattgatcac
     3001 caccgtgttg gccttggcca ccgccgcggg gatcggcacg gggccggcgc ccatcatcaa
     3061 tagcggttct tcgggaagaa tggcgtcgag cgattcgaca accgggcaag gcactgcggt
     3121 ggaaacagcg gaagcagagg gagtggatga agtcggcatg atctgtacgt gaatgtcagg
     3181 gcgcaaagga acaagcggcc ccgccagcaa tgacactggc ggggccgttc gtccgatcat
     3241 tcaccgatct gcgcgattgc gcaaggattt tgtcacatca ccttggtctt atcgagcttc
     3301 ttgccggttg ccgcgttata ccgctcgaca tagtcctcga tcagcttgcg catcgcgcgg
     3361 ccgatctgcc gatagtccga ttcattcaga ttagccagcg acacgcgtcc cgacggatgc
     3421 tgcgtaccaa aaccacgtcc cggcagcaac accacacgcg cttcacgcgc aaggcggaac
     3481 agcaactcgg acggctcggc gttcttcaac agccagtcga caaactcgcg cccgaacatc
     3541 cgctcgccga gaaactcgat gtcgacaatc gtgtagtagt cgacctggtt cggatcgtcg
     3601 tcgtcgaacg taatgccgac ctcctcgtac agcgcctgct tgcgtttgcg gatcaggcgc
     3661 ttcaacgcgt tcttgtacgc gtcaggcgtg tccatcagcg agaacaacga gaacagcacc
     3721 atctgcacct gctgcggcgt cgacaaaccg gccgtgtgat tcagcgcgac agtgcggctg
     3781 tcggcgacca ggcgatcgat gaatttcagc ttgtccggtt cggtcgtgat cgattcataa
     3841 cgctcgtgca actgcttctt ctcttctttt ggcagttcgc tgatcagctt gtcgagcacg
     3901 ttgtcgcgat gcgtcgcaat cgtgccgagg cgccagccgg tcgcgccgaa atacttcgag
     3961 tacgagtaca cgaggatcgt gttcttcggc gcgagcgcga acagcgagac gaaatcgtcg
     4021 gcaaaggtgc cgtacacgtc gtcggtcaac agaatcaggt cgggccgttc cttgacgatg
     4081 tcggcgatgt attgaaggct ctcgtcgttg atcttcaccg aaggcgggtt gcttggattc
     4141 acgaggaaga acgccttcac cttcggatcg cgcagcttgt cgagttcctt cttcgaatac
     4201 tgccagccgc tttcaaccgt ggcttcgaga ttgacaacgt tcagctgata gtcgttgagg
     4261 cgcggaattt caatgtacgg cgtgaagata ggcaagccaa gcgcgatcgt gtcgcccgcc
     4321 ttgatcaggt gattctcgcg catcgtgttg aagatgtacg tcatcgccgc cgtgccgcct
     4381 tccacggcga acacgtcgaa ctcgcccaca aacggatgat tgccgatcat ctccttgcgc
     4441 aggtactgcc cgacgataat ttcggacaac ttgagcatcc ggtccggcac cggatagttc
     4501 gaggcgagaa tgccctcgca catttcatag aggaagtcgc ccgcggacaa tccgagctga
     4561 tcgcgcacat acgagatcgc gccgcgcaga aagtcgatgc cgggcacgcc cttgttttcg
     4621 cgcaggaaca gatcgaagcg ttcggtgagt ccatcgcggc gcgggaagcc gcccacgccc
     4681 tccggcatga acgcaaatga acgctcggat tcgcgcatcg caaacaggcc gagttgccag
     4741 aagccgtggc gcggaatcgt cgcgagaaag ttggggttgc cgcggccggc gttgagcatc
     4801 gacgcattgg cgggacgctc caccgcgccg ccgcccgcgg ccttgatcag ttcgtccttg
     4861 agttcgaacg ggctgagtgc ttcaaagccg gcttgcgcca tgtcgccttg tttcttgccg
     4921 cctttctctt ttgccatgat gccttctcca atgtgcaagt aaatgacgcc ggcgtcgcga
     4981 gtcacgattc cattgccgcc ggcgtgagag agatcgaaag atgcgtgtgc taacccagcg
     5041 aaaacaggca gtacgaagct aatccgggct ataccagccc caccacgagc ggcccgagca
     5101 acgtcaatgc gacgttggca atcgcatacg tgatcgcgaa cggcacggtt ggtacggcgc
     5161 tttcggcttt gtcgagcacg ccgccaaatg ccggattggc gctgcgggaa ccggtcagtg
     5221 caccggccag aatggccgcg ttgttgtagc gcagcacgta acggccgaac aacatggtca
     5281 acaccagcgg cagcacggtc acgaccacgc cgagcagaaa gatcgtcacg ccgctctgct
     5341 tgacggtcac cacggcctgc aagcccgagt tcaaccccac caccgcgaca aacgccgcga
     5401 ggccgaagtc tttcaacagt tgcgaagccg cggacggcat cgcgccgtac atcggatgct
     5461 tgccgcgcat ccagccgaat agcaggccgg ctagcagaca gccgccgccg gaaccgagcg
     5521 tgagcggaac gccgcccacg tcgacgacga tcagaccgat cagcagaccc agcacgatgc
     5581 cgacgcccat ataaatgaag tcggtcttgt tgctgtacgg cagctcgtag ccggctgcct
     5641 cgacggcgcg cttggtgtct ttcggcgagc cataaaaagt gatgacgtcg ccgtgttcga
     5701 gtttcgtttc aggcaagacc ggcagcggct gaccggcgcg cgaggcgctc tgaatgtaga
     5761 caccgtggcg caggtcgcga tccaccattt cgcgcgcggc ggcgatcgtc gtgtggttca
     5821 tgcctttgcg ggtgaacaca ccgtcgcgcg tctgcatcac gacaccaatg tcctcgacgt
     5881 tggcgatctc gttgccgttc gagccgaacg cgactacggc ctcacggcgg cctaccacca
     5941 gcacttcgtc gttgagttgc aacgtgagat cgggcatcgg gtcgagggcc ttgccgtcac
     6001 gtctgatgcg ctcgatcgtc agcatgtcct gatgctgggt ctcgacagcc ttgaccgttt
     6061 tccccgcgct gacatcgatc ttgtatgcgc gtcccaccag ctcaggcaac gcccgaatct
     6121 gccccgcgcc gagtgcgggc gcgcctgacg acagttcccg ctccgcttcg atcgaggcgt
     6181 cgcgcaaact ctgccccatg aatttcggca ggatattcac gcagacgatg atcgcgccga
     6241 gcgagccgaa cacgtaggtc accgcgtagg caatcgcgac atccgattgc aacgacttga
     6301 cctcgtcgac gggcaaaccg aggcgcgcga tggcgtcgcc ggccgtgccg ataatcgccg
     6361 actgcgtcag cgcaccgccc gccagccccg cagcgaggcc tttgttgaga tggaacagct
     6421 tcgcgcatat caccacggtg atcagcgcgg tcaccgcgag aaagatcgcc atggcgatct
     6481 cacgcagcgt cttgcggttc aacgagttga agaagcccgg cccggagtcg tacccgaccg
     6541 cgtagatgaa aaccgcaaac atcaccgatt tcacgccgtt gtcgatcgtg acgccagcct
     6601 gactgatcac gaccgccgc
//
LOCUS       DATASH010000547         1513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_286846,
            whole genome shotgun sequence.
ACCESSION   DATASH010000547 DATASH010000000
VERSION     DATASH010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286846"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VHV99_15135"
     CDS             <1..298
                     /locus_tag="VHV99_15135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636791.1"
                     /translation="ATFSERVGTALVNGSWFVRKLMPFAARIVVMHGHRHIDWIGVCG
                     AMKVLSAPSPVMGATDDAPTHFHIHTFAAGLDGQLDLLQPERVDIPGVESRSAG"
     gene            complement(295..1335)
                     /locus_tag="VHV99_15140"
     CDS             complement(295..1335)
                     /locus_tag="VHV99_15140"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX3636792.1"
                     /translation="MVAGFAFKIAVAIITTVLLAAAAAQASAVFAQPALAIFIIAVVW
                     PLQHWLQTRMPRLLALAITIVITVAVCVAFASLAVWGFSRVGRSLVADTARYQALFDT
                     TVTWIDGHGVSVAGLWAEHFNVAWLLRATQHIAGRVNTTLSFWLIALVYVILGLLEVD
                     DIRRKIEGLDNREAARVLLDGSAATAKKFRKYMVVRTQMSAMTGLLVGAFAGLTGLPF
                     AIEWGVIAFVLNYIPFIGPFIATLFPTLLAMTQFDSWQAVLGVFICLNVIQFVVGSYI
                     EPRVSGDILSISPLVVLFAIFFWTFLWGLFGTFIGVPIALALVSFCGQHPSSRWLADL
                     LGGPKQTKPHKI"
BASE COUNT          329 a          458 c          463 g          263 t
ORIGIN      
        1 cgcgacgttt tccgaacgcg tcggcactgc gctcgtcaac ggcagctggt ttgtacgcaa
       61 gctcatgccg ttcgcggccc gcatcgtggt catgcacggc caccgccata tcgactggat
      121 cggcgtgtgt ggcgcgatga aggttctgtc cgctccgtcg ccggtgatgg gcgcgacgga
      181 cgacgcgccc acgcattttc atatccatac gttcgccgcc ggcctcgatg ggcagcttga
      241 tttgcttcag cccgagcgcg tcgatatccc cggcgtcgaa tctcgatcag cgggctagat
      301 cttgtggggt ttcgtctgtt tcggtccgcc gagcagatcg gcgagccaac ggctcgacgg
      361 gtgctggccg cagaagctga cgagagcgag ggcgatcggc acgccgatga aagtaccgaa
      421 caggccccac agaaaggtcc agaagaagat cgcgaacagc acgaccaagg gagagatcga
      481 aagaatgtcg ccagacacgc ggggttcgat atagctaccg accacgaact ggatgacgtt
      541 gaggcagatg aagacgccaa gaaccgcctg ccaactgtcg aactgcgtca tcgccagcaa
      601 agtcggaaac agcgttgcga tgaaagggcc gatgaacggg atatagttga gcacgaaggc
      661 gatcacgccc cattcaattg cgaatggcag cccggtgagt ccggcgaaag ccccgacgag
      721 caggccggtc atggcgctca tctgcgtccg caccaccatg tatttcctga acttcttcgc
      781 ggtcgctgcg ctgccgtcga gaagcacgcg cgcagcctcg cggttgtcga gtccttcgat
      841 cttccgcctg atatcgtcga cttccagcag tcccaggatg acgtagacta gcgcgatcag
      901 ccaaaagctt agcgtggtgt tgacgcggcc agcaatgtgc tgtgtcgcac gcaacagcca
      961 cgcgacgttg aaatgttcag cccacagccc ggcgactgag acgccgtgac cgtcgatcca
     1021 tgtcaccgtc gtgtcaaaca gtgcctggta gcgtgcggtg tctgcgacga gcgatcgtcc
     1081 cacccggctg aagccccaga ccgccagcga ggcaaaggca acgcacaccg cgaccgtgat
     1141 gacgatcgtg atcgccagcg ctagtagcct cggcatccgc gtctgcagcc agtgttgcaa
     1201 gggccaaacg accgcaatga tgaagattgc cagtgcgggt tgtgcaaata ccgcgctcgc
     1261 ctgcgcggcg gcggccgcga gcagcaccgt cgtgataatc gcgacggcga tcttgaatgc
     1321 gaagccggct accattcttg agagctttcg aaaaaatgcc gtgctgctgc cgaagacgat
     1381 ggcaagctta gagcttcgac ggcagcccgg caacacgacc cagcggcgag cccgggcatc
     1441 tgattagaat tgtcagcaat gcgaactcac ccacgaaccg aaaagcccga attcgcagga
     1501 atagcgattg tgg
//
LOCUS       DATASH010000548         1573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2661875,
            whole genome shotgun sequence.
ACCESSION   DATASH010000548 DATASH010000000
VERSION     DATASH010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2661875"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(231..>1573)
                     /locus_tag="VHV99_15145"
     CDS             complement(231..>1573)
                     /locus_tag="VHV99_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3636793.1"
                     /translation="SGNWRALLACFLYFDTGFTVWVMFGPLAPFIHKDIAMSPAELGF
                     LVAVPVLGAAILRVTLGNLYQAYDGRRIALWGVVLSALPSIVLLLSPSASSYVMLLVL
                     GVFLGVGGASFAVALPMAGSNYPPKVQGLVLGLAAAGNIGAVLDGFLFPQLATHYGWQ
                     MATVGALPLLAIAALTLYFWANDSGVKSGNALRAFGSFVVTLAGLIVLVLLVEAGLFG
                     SGKTGVLLLPVIGALLAIAVLPRRYRSVLAERDTWAIMLVYGITFGGFVGMSSYVSLL
                     LTNLYQLSKIDAGLFMALLAATGAMVRPLGGLIADKVSGVRALTFLLAIISLCDFVFA
                     AAMPSMVGGIALLLCLYVAFGLGNGATFQLVPHRWAGRTGLMSGIVGAAGGIGGFYLP
                     VVMGIAKESTGSYQMGFATFGALAACAFLAIVALRRPWMAWSMHTGVIHAHATE"
BASE COUNT          376 a          536 c          466 g          195 t
ORIGIN      
        1 tgagtgacct cggagaggct gccgagatcg gagaattccg ctttggcggt tgtccagacc
       61 atgtgccacc agttggccat tacatttacc tgacattcgg aaatgtgccc agcatagctt
      121 gtgaacacac cggtcgggca gatggaagcg aaacaaatgc aacaggcggc acggctaacc
      181 caacaaactt acctcaccga agtttcgtca caaacgcgcg caccacagcc tcactccgtt
      241 gcgtgcgcat gaatcacgcc ggtgtgcatc gaccacgcca tccacggacg gcgcagcgcg
      301 acgatcgcga ggaacgcaca agccgccaac gcgccgaagg tcgcgaagcc catctgatag
      361 ctgcccgtgc tttccttcgc gatgcccatc acgaccggca gatagaatcc gccgatgccg
      421 cccgcggcgc cgacgatacc cgacatcaag ccggtgcggc cggcccagcg atgcggcacc
      481 aactggaagg tcgcgccatt gccgagaccg aaggccacgt acaggcacag cagcagcgca
      541 attccgccca ccatcgacgg catcgcggca gcgaacacga aatcgcacag cgaaatgatc
      601 gcgaggagga aggtcagcgc gcgcacgccg gacaccttgt cggcaatcag gccgccgagc
      661 ggccgcacca tcgcgccggt cgcggcaagc agcgccatga aaagacccgc atcgatcttc
      721 gagagctgat agaggttggt cagcagcagc gaaacatacg acgacatgcc gacaaaacca
      781 ccgaacgtga tgccgtacac cagcatgatc gcccacgtgt cgcgctccgc cagcaccgag
      841 cgatagcgcc tgggcagcac ggcgatcgcc agcagtgcgc cgatcaccgg caacagcagc
      901 acaccggtct tgcccgaacc gaacagaccc gcctcgacca gcagcacgag cacgatcaaa
      961 ccggccagcg tcaccacaaa gctgccgaat gcgcgcagcg cgttgcccga tttgacaccg
     1021 gaatcgttgg cccagaaata cagcgtgagc gcggcaatcg cgagtagcgg caatgcgccg
     1081 acggtcgcca tttgccagcc gtagtgcgtt gccagctggg ggaacaggaa gccgtcgagc
     1141 accgcgccga tgttgccggc agcggcaaga cccagcacca ggccctgcac tttaggcgga
     1201 tagttgctgc cggccatcgg cagcgccacc gcaaagctcg ctccgcccac accgagaaac
     1261 acacccagta ccagcagcat cacgtatgag gacgcggacg gtgacagcag cagcacgatc
     1321 gacggcaacg ccgacagcac gacgccccag agcgcaatgc ggcggccatc ataggcctga
     1381 tacaggttgc cgagcgtcac gcgcaggatc gccgcgccca gcaccggaac cgcgacgaga
     1441 aagcccagtt ccgccggcga catcgcgatg tccttgtgga tgaacggggc gagcgggccg
     1501 aacatgaccc acacggtgaa gccggtatcg aagtagagga agcacgccag caacgcgcgc
     1561 cagttaccgc tcg
//
LOCUS       DATASH010000549         4789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3257144,
            whole genome shotgun sequence.
ACCESSION   DATASH010000549 DATASH010000000
VERSION     DATASH010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4789
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3257144"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1188)
                     /locus_tag="VHV99_15150"
     CDS             complement(<1..1188)
                     /locus_tag="VHV99_15150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006026902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClcB-like voltage-gated chloride channel
                     protein"
                     /protein_id="HEX3636794.1"
                     /translation="MLSFLLKLRTRAQHLFRLSDTHTMLVWSVVVGVAGAFATIAFRE
                     GIALLQLATVGKAGSFVEMARSLPWTVRVWLPAAGGLIAGFFLLIAQRHVDKSNHIDY
                     MEAVAIGDGVVPVKLSLWRSVSSLFTISSGGSIGREGPMVQLAALAASLIGRLVHFDP
                     PRLRLLVACGAAAGITSAYSAPIAGAFFVTEIVLGSIVMESFGPVVVSAVVANITMRE
                     FTSYKPPYEMPVFPPVAGAEVLLFVALGLICGAAAPQFLRLMDLSKANFRKLPLPLPI
                     RLALGGLVVGILSVWTPEVWGNGYSVVNSILHSPWTWTALVLVLVFKIVATSATVGSG
                     AIGGVFTPTLFVGAVVGCLFGQGMHALWPHGTSAPFAYAMVGMGAFLAGATQAPLMAI
                     LLIF"
     gene            complement(1301..2038)
                     /locus_tag="VHV99_15155"
     CDS             complement(1301..2038)
                     /locus_tag="VHV99_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018435580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEX3636795.1"
                     /translation="MSLELYYWDGLQGRGEFVRLALEEAGADYVEVARGEASDGLGTH
                     AMMAVMKSRDEPYPPFAPPFLKDGDLVIAQTANILFYLGPRLKLAPSVESLRYVANGL
                     QLTIADVVAEAHDTHHPLASGLYYEDQKDAAKVRAHDFIDNRIPKFMKYFERVLKQNP
                     AGDTCMVGDALTYVDLSMFQLIDGLLYAFPRALKRFGEHYPRLAALHDAVIERPNIAA
                     YLRSERRIEHNEACIFRHYPELDKAAT"
     gene            complement(2184..3284)
                     /locus_tag="VHV99_15160"
     CDS             complement(2184..3284)
                     /locus_tag="VHV99_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HEX3636796.1"
                     /translation="MSTAHLTAAASSAGSSGSSTRGQLNGILFVALFAAAVTRIAAIP
                     AIAGLGLSPLIVGIVAGALYGNALRDSMPASWAAGVNFSARKLLRIAVAFFGLRVSLQ
                     EIAQVGLPGLAESVLIVVSTLVIGTWAGMKIMKLDRDTALLTAAGSAICGAAAVLAFE
                     STLQSKPHKSAMAVGSVVLFGTLSMFLYPVLFKAGWLHLDTVGAGLFFGGTIHEVAQV
                     VGAASNVSPEATHIATIVKMTRVMLLVPVLLVVGMWVNRSARSEAATATAEGGKQPAP
                     RKLAIPWFAVGFLAFVVINSLHVLPESATGTLNMLDTFALTMAMTALGIETRIAQIRQ
                     AGPRALTTGFILYVWLVAGGLGITWTVQHLFG"
     gene            3406..4398
                     /locus_tag="VHV99_15165"
     CDS             3406..4398
                     /locus_tag="VHV99_15165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3636797.1"
                     /translation="MTPDQLITFAAVAEHRNISRAAVALHLSQPAVSGQLRQLQDEFG
                     EPLYQRDGRGVRLTPAGEQLASYAARLRDTWRQAHAYRDALRGLEQGTLRIGASTTPA
                     SYLLPYLIADFHRLYPEVTLHTADGNTTEIVGALGSVDIAMIEGPVGADLPPDTAVHA
                     WREDEIVAIMPRTHPLAVALAGVTDSAGAVAGAAETGVAGRATAVGAGVNGGRAELAA
                     FGPHPLVLREAGSGVRQIVERAFARAGVPMRVALEIAGVEGVKEAVRAGMGIGFVSAM
                     SMRHEDGALCLFSLSPAPLTRRLSILVPHASAPSRVVERFLALCLADGPGASAA"
BASE COUNT          882 a         1497 c         1619 g          791 t
ORIGIN      
        1 gaagatcaac aggatcgcca tcagcggcgc ctgcgtggcg ccggccagaa acgcgcccat
       61 gccgaccatc gcgtaggcga acggcgccga ggtgccgtgc ggccacaacg catgcatgcc
      121 ctggccgaac aggcaaccca ccaccgcgcc gacgaacagc gtcggcgtaa acacgccgcc
      181 gatcgcgcct gaacccacgg tcgccgacgt cgcgacgatc ttgaacacga gcacgaggac
      241 gagcgcggtc catgtccacg gcgaatgcag aatggagttg accacgctgt agccgttgcc
      301 ccacacctcg ggcgtccaca ctgacagaat gccgaccacc agaccgccca gcgccagccg
      361 gatgggcagc ggcagcggca gcttgcgaaa gtttgccttc gagaggtcca tcagccgcag
      421 aaattgcggc gcggccgcac cgcatatcag cccgagcgcg acgaacagca ggacttcggc
      481 gcctgccacc ggcgggaaca cgggcatttc atacggcggc ttgtagctgg tgaactcgcg
      541 catcgtgatg ttggcgacca ccgccgagac caccacgggg ccgaagctct ccatcacgat
      601 cgagccgagc acgatctcag tgacaaaaaa cgcgccggcg atcggcgcgc tgtaggcgga
      661 ggtgatgccg gccgcggcac cgcacgcgac gagcaggcgc agacgcggcg gatcgaaatg
      721 caccaggcgg ccgatcagcg acgcggccag cgccgccagt tgcaccatcg gaccttcgcg
      781 gccgatcgag ccgccgctcg aaatcgtgaa cagcgacgac acgctgcgcc acaaactcag
      841 tttgaccggt accacgccat cgccgatggc gaccgcttcc atgtagtcga tgtgattgct
      901 cttgtcgacg tgacgctgcg cgatcagcag aaagaagccg gcgatcaggc cgcccgcggc
      961 gggcagccac acgcgcaccg tccacggcaa gctgcgcgcc atttcgacga aactgccggc
     1021 cttgccgacc gtcgccagtt gcagcaatgc gatgccttcg cgaaacgcaa tcgtcgcgaa
     1081 ggcgcccgcg acgccgacca ccaccgacca gaccagcatc gtatgggtgt cggacagacg
     1141 gaacaggtgt tgcgcgcggg tgcgcagctt cagcaggaat gaaagcacgt cggcagtaga
     1201 gggcgtgatg ggtggatgaa atgcgcgtac ggcaaccgca gcgcgcccgc tctcgaaagc
     1261 gggcgcgctg ctgagggttc gcgaaggagg ccggcgtggc tcaagtcgcc gccttgtcga
     1321 gttccgggta gtgccggaag atgcaggctt cgttgtgctc gatgcgccgc tcggaccgga
     1381 gataagcggc gatgttcggc cgctcgatca ctgcgtcgtg cagcgccgcg agacgcggat
     1441 agtgttcgcc gaaacgcttg agcgcacgcg gaaacgcgta cagcagaccg tcgatcaact
     1501 ggaacatcga caggtcgacg taagtgagcg cgtcgcccac catgcaggtg tcaccggccg
     1561 ggttctgttt cagcacgcgc tcgaagtact tcatgaactt cggaatccgg ttgtcgatga
     1621 agtcgtgcgc gcgcactttg gcggcgtctt tctggtcttc gtaatagagg ccgctcgcga
     1681 gcgggtgatg tgtgtcgtgc gcttcggcca ccacgtcggc gatcgtcagt tgcaggccgt
     1741 tggccacgta gcgcaggctt tcaaccgatg gcgcgagctt gagccgcggg cccaggtaga
     1801 acaggatgtt ggcggtctgc gcgatcacca gatcgccgtc tttcagaaac ggcggcgcga
     1861 acggcgggta gggctcgtcg cggcttttca ttaccgccat catcgcgtgg gtgcctaaac
     1921 cgtccgaggc ctcgccacgc gccacttcca catagtccgc gccggcttct tccagggcca
     1981 gccgcacgaa ttcgccacgg ccctgcaggc cgtcccaata gtaaagttcc aggctcatcg
     2041 atcgatcctc attccggttg catgaacggg tttgcgcgaa ctgacgacgt cagcaatcag
     2101 tatgccgtgc aagcgtgcgg gatggttctc ctgaacgcac aaaaccccgc acgcggcggg
     2161 gttggcgggg aggcgaggac ggcttagccg aacaggtgct ggacggtcca tgtgatcccc
     2221 agtccgccgg caaccaacca cacatacaga atgaagccgg tggtcaaggc gcggggtccg
     2281 gcctgacgga tctgcgcgat gcgggtttcg atgccgagcg cggtcatggc catcgtcaac
     2341 gcaaaggtgt cgagcatgtt cagcgtgccg gtcgcgcttt cgggcagcac atgcagcgag
     2401 ttgatcacca cgaaagcaag gaagccgacc gcgaaccagg gaatcgccag cttgcgcggc
     2461 gccggttgct tgccgccttc agccgttgcg gtggcggctt cgctgcgtgc cgagcggttc
     2521 acccacatgc cgacgaccag cagcaccggc accagcagca tcacgcgggt catcttgacg
     2581 atggtcgcga tgtgagtcgc ttccggactg acgttgctgg ccgcgccgac cacctgcgcc
     2641 acttcgtgaa tcgtgccgcc gaagaacagg cccgcgccga ccgtgtcgag atgcagccag
     2701 cccgccttga acagcaccgg gtagaggaac atcgacagcg tgccgaacag cactacgcta
     2761 cccaccgcca tggcgctctt gtgcggcttc gattgcagcg tcgactcgaa tgcgagcacc
     2821 gcagcagcac cgcagatcgc gctgcccgca gccgtgagga gtgccgtgtc gcggtcgagc
     2881 ttcatgattt tcatgcccgc ccacgtgccg atcaccagcg tgctcacgac aatcaatacc
     2941 gattcagcga gaccgggcaa gccgacctga gcgatttcct gcaagctgac gcgcagcccg
     3001 aaaaatgcca cggcgatgcg caggagcttg cgcgccgaga agttgacgcc ggccgcccag
     3061 cttgcgggca tgctgtcgcg tagcgcgttg ccgtaaaggg cgcctgccac gatgccgacg
     3121 atcagcgggc tcaagcccaa cccggcgatg gccgggatgg cggcgatgcg ggtcacggcc
     3181 gcggcgaaca gcgcgacgaa cagaatgccg ttgagctggc cgcgggtgga ggaccccgaa
     3241 gaaccggcag atgacgcggc agcggtgagg tgagcagtag acatggcgta gcgccttcct
     3301 atgacgggtc ttgattcgat aggccaaatc ctaaattaga tatatcgata tgaaaaatca
     3361 tgatttagga tgtaaagtat cggttacacg gatactttcc accacatgac cccggatcaa
     3421 cttataacgt tcgctgccgt cgccgaacat cgcaatatca gccgggcggc tgtggcgctg
     3481 catttatcgc agccggccgt ctctggacaa ttgcggcagt tgcaggacga attcggcgag
     3541 ccgctttacc agcgcgacgg gcgcggcgtg cgcctgacgc ctgccggcga gcagctcgcc
     3601 agctatgcgg cccggctacg cgacacgtgg cgccaggcgc atgcgtatcg cgacgcgttg
     3661 cgcggcctcg agcaaggcac cttgcgcatc ggcgcgagca ccacgccggc gagctatctg
     3721 ctgccttacc tgatcgctga ctttcatcgc ctctatcccg aggtgacgct gcataccgcg
     3781 gatggcaaca ctacggagat cgtcggcgcg ctgggctcgg tggatatcgc gatgatcgag
     3841 gggccggtcg gcgcggatct gccgccggac acggcggtgc atgcgtggcg ggaagacgaa
     3901 atcgtcgcga tcatgccgcg cacgcatccg ttggcagtgg cgttggctgg tgtgacggac
     3961 agcgcggggg cggtcgcggg ggcggcagaa acaggcgtgg caggacgagc aacggcagta
     4021 ggagcaggcg tgaacggcgg gcgggcggaa ctggcggcat tcggcccgca tccgctggtg
     4081 ttgcgcgagg ctggttcggg cgtgcggcag atcgtcgagc gggcctttgc tcgcgcgggc
     4141 gtgccgatgc gcgtggcgct ggaaattgcc ggggttgaag gtgtcaagga ggcggtgcgt
     4201 gcgggaatgg gcatcggttt cgtatcggcg atgtcgatgc ggcatgaaga cggcgcgctg
     4261 tgcctgttct cgttgagccc ggcgccgctg acgcggcggc tgtcgattct ggtgcctcac
     4321 gcgagtgcgc cgtcgagggt ggtggagcgg tttctcgcgt tatgcctcgc ggatgggcct
     4381 ggcgctagtg cggcttagcg gcgaccccgg cttgcgggat caaggtcgct tggcggtgac
     4441 ttcggcttgc gggatcgagg tggcttagcg acgacttcgg cttgcgggat cgaggtggcc
     4501 tggcggcgac ctcggcttgc gggatcgagg cggcttagcg gcgacctcgg cttgcgggat
     4561 cgaggcggct tagcgctgcc tcggcttgcg ggctcaaggc ggcttagcgg ccacctcggc
     4621 ccgcgagacc aaggcgcccc gcagccagcg tgcgcagttt ccacttcatg ccaagttcgc
     4681 ctggggattt ccactatggt gcatgccaca tggctcggcg cactattcgg gcatcggagc
     4741 gcttcggcgc tttttttcga aacgtgtttg gaaccggttc caaatgccg
//
LOCUS       DATASH010000550         2875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3257238,
            whole genome shotgun sequence.
ACCESSION   DATASH010000550 DATASH010000000
VERSION     DATASH010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3257238"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..463)
                     /locus_tag="VHV99_15170"
     CDS             complement(29..463)
                     /locus_tag="VHV99_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636798.1"
                     /translation="MKRFFSCVLLALAAITLPLASSAAALDAPLNCNESGHQFIADLA
                     QQQMIDPKPTRVESNSINAFSPVHGADLTAFGFRIFAVVGYEKDDPLFRVGDGEPVAR
                     SAYGVVVFGSESKVQSALAEAGSTAIVHRVAPLVTAIFCKRS"
     gene            718..1413
                     /locus_tag="VHV99_15175"
     CDS             718..1413
                     /locus_tag="VHV99_15175"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX3636799.1"
                     /translation="MNSRPSIPTNVPAPRTWDARLARRLVTPLVNTWVTPNHLTTLRL
                     LIGLAGALCLARGGFAWANSGAFLIVLSNFVDHTDGELARIGGKSSRIGHFYDLACDA
                     LVTVMLFVGMGIGSTHVSALNVAPGWLGAVAGVAIALIFFLRMRIEDMAGKAGTKQAS
                     AGGFETEDVLYLLPIVTLTSVVTPFVVLASIGAPLFAAWVVVDYWRVARRTARSSVAP
                     AKASETSQMWASE"
     gene            1410..2231
                     /locus_tag="VHV99_15180"
     CDS             1410..2231
                     /locus_tag="VHV99_15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018423588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEX3636800.1"
                     /translation="MSTHAEDDVIASMSVERSPASAQAAPAPNADRAVASRTRTFDTR
                     RFSREFTDQGAFLYLEDFLAPEVTAQLVQSARGLLDEVNRNYLPGHKQGGSVSRHTID
                     RLAPFIAELYRSKELIGWLEQLSGDKLQVSPADDPHAYALYYYTRAGDHIGWHYDTSY
                     YDGRRYTLLLGVIDESSCRLDYELHTRNPDVPDQPGSVQIPPGGLVFFDGDKLRHRIT
                     PAGANEMRVSLTFEYVTDPNMRPWRRFISNMKDAIAYFGFRQVFRQMTKRGKDHA"
     gene            2228..>2875
                     /locus_tag="VHV99_15185"
     CDS             2228..>2875
                     /locus_tag="VHV99_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006399831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEX3636801.1"
                     /translation="MTRAALILLSIGTALFVGLLAWQGFGSIASALAAAGWGLVLVAA
                     FHLVPLVLDAGAISVLFQRQRDGVHHDVTLRDALFAPWIGESVNSLLPAGQIGGPLVM
                     VRQLSQRGMRMRDAAAAITVSTTAQALAQIVFALLGLLLFGAYAAHGALHDLRTATLI
                     ATGVLGAMIAGFYYAQHRGLFGRLLGVLSKVFGKRDWSSLMTRAEAVDAAVQAMYR"
BASE COUNT          495 a          924 c          905 g          551 t
ORIGIN      
        1 ctactggcct tgcggtgcga ttgaccgctc aactccgctt gcagaaaatc gccgtcacga
       61 gcggcgccac gcgatgcacg atcgccgtgc tgccggcttc ggccagcgcg ctctgcacct
      121 tggattcgct gccgaacacc acaacgccgt aggccgagcg ggccaccggt tcaccgtcgc
      181 cgacgcgaaa cagcggatcg tctttttcgt agccgaccac cgcaaaaatg cgaaacccaa
      241 acgcggtcaa atcagcaccg tgcacgggcg aaaacgcatt gatggagttg ctttcgacgc
      301 gcgtcggttt cggatcgatc atctgctgct gggcgagatc ggcgatgaac tgatgaccgc
      361 tctcattgca gttcagcggc gcatcgaggg ccgcggcgga gctggcgagc ggcagcgtga
      421 tggcggccag cgcaaggagt acacacgaaa agaagcgttt cataggcgtg aatggcgagc
      481 cgtacgtatt cgaagtagtg cgtagtgtgt tcgcaacggg tagcggcggc aatcgctcgc
      541 ggcccgaaag acgttgcgca tcgaagtaat ccggcgtgac aggcgcaact ggattcgcac
      601 tttaacgtga ttcgacaaat tttgccctct gcctcgcggg cgtcggagac gaatggccct
      661 tcagaaaacg ttaaggaata aagcaagctt tggcgatccc gtatatattt ccgggttatg
      721 aattcgcgac catcaattcc aactaacgtt ccggccccca gaacatggga tgccaggctg
      781 gcccgccgac tcgtcacgcc gctcgtcaac acctgggtca cgccgaacca cctcaccacg
      841 ctgcgcctcc tgatcgggct cgccggcgcg ctatgcctcg cgcgcggggg ctttgcctgg
      901 gctaactcgg gcgcctttct gatcgtgctg tcgaacttcg tcgaccacac ggacggcgaa
      961 ctggcgcgca tcggcggcaa gtcgagccgg atcggtcatt tttacgatct ggcctgcgac
     1021 gcgctcgtga ctgtcatgtt gttcgtcggc atgggtattg ggtcgacgca tgtcagcgca
     1081 ttgaacgttg cccccggatg gctgggtgcc gtggcgggcg ttgcaatcgc attgattttc
     1141 ttcctgcgca tgcgcattga ggatatggcg ggcaaggcgg gtaccaaaca ggcatcggcg
     1201 ggcggtttcg aaactgaaga tgtgctgtat ctgctgccca tcgtcacgct gaccagcgtc
     1261 gtcacgccgt tcgtggtgct cgcgtcgatc ggcgcgccgc tttttgctgc ctgggtggtg
     1321 gtcgattact ggcgtgtcgc gcgccgcacc gcacgctcgt cggtcgcccc cgccaaggcg
     1381 tctgaaacca gtcaaatgtg ggccagcgaa tgagtacgca cgccgaagac gacgtgatcg
     1441 catcgatgtc cgttgaacgt tcgcccgcgt cggcgcaggc cgcgcctgcg ccgaacgccg
     1501 accgagccgt tgcgagccgc acgcgcacgt tcgacacgcg gcgtttctcc agagaattca
     1561 ccgaccaggg cgcgttcctg tacctggaag atttcctcgc acccgaagtc accgcccagc
     1621 tcgtccagag cgcgcggggt ctgctcgacg aagtcaatcg caactatctg ccggggcaca
     1681 aacagggcgg cagcgtgagc cgccatacga tcgaccgtct cgcgccgttc atcgccgagc
     1741 tgtatcgctc gaaggaactg atcggctggc tcgagcaact gagcggcgac aagctgcaag
     1801 tctcgcccgc ggacgatccg cacgcctatg cgctgtacta ctacacacgc gccggcgacc
     1861 acatcggctg gcactacgat acttcgtact acgacggccg ccgctacacg ctgctgctcg
     1921 gcgtgatcga cgaatcgtcg tgccggctcg attacgaatt gcatacgcgc aatccggacg
     1981 tgccggacca gccgggttca gtgcagattc cgccgggtgg cctggtattc ttcgacggcg
     2041 acaagctgcg ccatcgcatc acgccggccg gcgcaaacga aatgcgcgtg tcgctgactt
     2101 tcgaatacgt gaccgacccg aacatgcggc cgtggcgccg tttcatctcg aacatgaagg
     2161 acgcgatcgc gtacttcggt ttccgccagg tgttccgtca aatgacgaag cgcggcaagg
     2221 accacgcatg acacgcgcgg ccctgattct gctgtcgatc gggacggcgc tttttgtcgg
     2281 cctgctcgca tggcagggtt tcggttccat cgcctctgcg ctggccgcgg ccggctgggg
     2341 gctcgtgctc gtcgcggcgt tccacctcgt gccactggtg ctcgacgccg gcgcgatttc
     2401 ggtgctgttc cagcgccagc gcgacggtgt ccatcacgac gtcacgttgc gcgacgcctt
     2461 gttcgccccc tggatcggcg aatcggtgaa tagcctgttg ccggccggtc agatcggcgg
     2521 cccgctggtg atggtgcggc aactgtccca gcgcggcatg cgcatgcgcg acgcggccgc
     2581 cgcgatcacc gtcagcacca ccgcgcaggc gctcgcgcaa atcgtcttcg cgctgctcgg
     2641 gctgctgctg ttcggcgctt acgccgcgca cggcgcgctt cacgatctgc gaaccgccac
     2701 actcattgcg accggcgtgc tgggcgcgat gatcgcggga ttctattatg cgcagcaccg
     2761 cggcctgttt ggccgtttgc tgggcgtgct ctcgaaggtg ttcggcaagc gcgactggtc
     2821 gtcgctgatg acccgcgcgg aagccgtcga cgccgccgtg caggcgatgt accgc
//
LOCUS       DATASH010000551         7158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4836577,
            whole genome shotgun sequence.
ACCESSION   DATASH010000551 DATASH010000000
VERSION     DATASH010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7158
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4836577"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VHV99_15190"
     CDS             complement(<1..363)
                     /locus_tag="VHV99_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX3636802.1"
                     /translation="MKLLRYGPKGQEKPGLLDAQGKIRDLSKVVPDIDGAALSDESLA
                     KLRALDPASLPVVEGNPRIGPCVGKIGKFICIGLNYADHAAESNLPVPTEPVIFNKWT
                     SAINGPNDDVEIPRGSKKT"
     gene            complement(445..1188)
                     /locus_tag="VHV99_15195"
     CDS             complement(445..1188)
                     /locus_tag="VHV99_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3636803.1"
                     /translation="MTQRLAGKTALITAAGQGIGLATAELFAREGARVIATDIRIDGL
                     AGKPVEARKLDVRDDTAIKALAAELGAVDVLFNCAGFVHAGSILECTDEDWDFAFDLN
                     AKAMYRMIRAFLPAMLENGGGSIINMSSAASSVKGVLNRFAYSASKAAVIGLTKSVAA
                     DFITRGVRCNAICPGTVASPSLEQRIVEQAKAQGATLEAVQAAFVARQPMGRIGKPEE
                     IAALALYLASDESSFTTGHAHVIDGGWSN"
     gene            complement(1278..2108)
                     /locus_tag="VHV99_15200"
     CDS             complement(1278..2108)
                     /locus_tag="VHV99_15200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3636804.1"
                     /translation="MPIDAHQHYWDPARGDYEWLTPELKILYRPFGPADLKPLREKAG
                     IERTVVVQAAPTIDETRYLLDIARNEPSIAGVVGWVPLLLPNAPAVIEALAHEPKFKG
                     VRPMLQDLPDDTWIANPDLAPAIEALIAHDLTFDALIYARHVEPFETFAARFPTLRVV
                     VDHGAKPPIRYGRAGWQTWADAITRLARLPQVHCKLSGLVTEASPGWTEETLQPYVDH
                     LLASFGPARLMWGSDWPVLDLNGDYLLWHSVANTLLASLSDDEREAVFGGNAAAFYRL
                     "
     gene            complement(2116..3156)
                     /locus_tag="VHV99_15205"
     CDS             complement(2116..3156)
                     /locus_tag="VHV99_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX3636805.1"
                     /translation="MTTTGGSKIEQRRGIGRRTLQVTGLGLGTAPLGGLYRDLSDEEA
                     HATIAAAWDTGVRYFDTAPHYGHTKAEHRLGDALRDYPRSEYVLSTKVGRRFVPRTTP
                     YDGSEGWQDPLPFQAIYDYTHDGILRSFEDSQQRLGIVDIDILLVHDIGRFTHGDKNP
                     HYWRQLTEGGGFRALDDLRSSGAVKAVGLGVNEGAVILDAMAEFDIDCALLAGRYTLL
                     EQTTLDDLLPACEERGVSILLGGAFNSGILARGVEGDLKFNYGDAPQEVIERVARLEA
                     VCRVHGVPLAAAALQFPYAHSAVATVLTGARSADELRENAASFEQPIPADLWSALRDK
                     GLLDSRAPAPED"
     gene            complement(3425..4738)
                     /locus_tag="VHV99_15210"
     CDS             complement(3425..4738)
                     /locus_tag="VHV99_15210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HEX3636806.1"
                     /translation="MTDTSIASADARSPMLQGYLRSDGRKGIRNVVAVAYLVECAHHV
                     AREIVTQFREPLDAFDDPSAEREPPVHLIGFPGCYPNGYAEKMLERLTTHPNVGAVLF
                     VSLGCESMNKHYLVDVVRASGRPVEVLTIQEKGGTRSTIQYSVDWVRDARRQLAGQQK
                     VPMALSELVIGTICGGSDGTSGITANPAVGRAFDHLIDAGATCIFEETGELVGCEFHM
                     KTRAARPELGDEIIACVAKAARYYSILGHGSFAVGNADGGLTTQEEKSLGAYAKSGAS
                     PIVGIIKPGDVPPTGGLYLLDVVPDGEPRFGFPNISDNAEIGELIACGAHVILFTTGR
                     GSVVGSAISPVIKVCANPATYRNLAGDMDVDAGRILEGRGTLDEVGREVFEQTVAVSL
                     GAASKSEALGHQEFILTYKSFEPVGPACLPSNAAPLHRVVAIEPH"
     gene            complement(4781..5125)
                     /locus_tag="VHV99_15215"
     CDS             complement(4781..5125)
                     /locus_tag="VHV99_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HEX3636807.1"
                     /translation="MTSRPLQTPQTSPTDSRLILLSPTDNCLIAAARLEAGTQVEIEG
                     ERVTLTRTIDLGHKVARHELARDDKVLRYGAVIGHVTEAVARGAHLHTHNLESDYLPT
                     YTHDAGHEFVHH"
     gene            complement(5151..6209)
                     /locus_tag="VHV99_15220"
     CDS             complement(5151..6209)
                     /locus_tag="VHV99_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3636808.1"
                     /translation="MKNSVPSPAFGTAQAQPAQPQTAAQATRGKPARSEFARLRELAL
                     LPALALLIVIGAFVSPSFLTKANLVSVLGASAALALVVLAESLIVLTGKFDLSLESTV
                     GIAPAVGAMLVMPAASAGFGMQWPAAAGLLAIIVVGAVIGFVNGFLVVRLRLNAFIVT
                     LAMLIVLRGMLVGATKGGTLFDMPPSFFALATTIVLGLPLSVWLAAAAFAIAAFVLRY
                     HRLGRALYAIGGNPEAARAAGIRVERITWGVFVLGSILASVGGLIVTGYVGAINANHG
                     NGMIFTVFAAAVIGGISLDGGKGTMFGALSGVLLLGVVQNLLTLAQVPSFWIQAIYGA
                     IILGSLMVARLASGEGQN"
     gene            complement(6199..>7158)
                     /locus_tag="VHV99_15225"
     CDS             complement(6199..>7158)
                     /locus_tag="VHV99_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3636809.1"
                     /translation="RISELQREGVTFLFISHHLQEVYEICQAVTVLRDARHIVSAPVC
                     ALPREQLIEAMTGERGGLAVADAAARDALSVDTAIALDVKDLAGPDYEGVSFTVKRGE
                     VVGLTGATSSGRTSVAEAIAGLRAPKSGAISVDGMRLPPGDVPAALAHGIGCVPKDRH
                     HEGLVLTQSVAENASMTIARLLGKFGIAPPAKKHAFGQKMIDALGIVAQGPEHIVSGL
                     SGGNQQKVVMARALATDPNILVLIDPTAGVDVKSKEALLSVVDRVREDGKAVLVVSGE
                     LDDLRTCDRVLVMFRGRVAAEFPAGWQDHDLIASVEGVSLHEE"
BASE COUNT         1331 a         2356 c         2138 g         1333 t
ORIGIN      
        1 ggtcttcttc gagccgcgcg ggatttcgac gtcgtcgttc gggccgttga tcgcgcttgt
       61 ccacttgttg aaaatgaccg gctcagtggg caccggcaga ttcgattcgg ccgcgtggtc
      121 cgcgtagttc agcccgatac agatgaactt gccgatcttg ccgacgcacg gtccgatacg
      181 cgggttgcct tccacgaccg gcagcgaggc cggatcgagt gcgcgcagtt tggcgaggct
      241 ttcgtcgctc agcgcggcgc cgtcgatatc gggcaccact ttcgacagat cgcggatctt
      301 gccctgcgca tcaagcaagc ccggcttttc ctggcccttc ggcccataac gaagcagttt
      361 catcgttgca cttcccttac gttcagtgat atttggttcg tgtgtcagtg tttggcaatg
      421 tcttgcgctg tcttgcggcc caactcagtt cgaccagccg ccgtcgatca catgagcatg
      481 ccccgtggta aacgacgatt cgtcggacgc gagatacagc gccagcgcgg caatctcttc
      541 cggcttgccg atgcggccca tcggctgacg cgcgacaaag gccgcctgca cggcttccag
      601 cgtcgcgccc tgcgccttgg cctgctcgac gatccgctgt tcgagcgacg gcgaagccac
      661 cgtgcccggg cagatcgcgt tacagcgtac accacgcgtg atgaagtccg cagcaacgga
      721 cttggtcaac ccgatcaccg cggcttttga cgcgctgtac gcaaagcggt tcagcacgcc
      781 cttcacgctc gaggccgccg acgacatatt gatgatcgac ccgccgccgt tctccagcat
      841 ggccggcaaa aacgcgcgga tcatacgata catcgctttc gcgttcaaat cgaaggcgaa
      901 gtcccaatcc tcgtcggtgc attcgagaat cgaaccggcg tgcacaaagc ccgcgcagtt
      961 gaacagcacg tcgaccgcgc cgagttcggc cgccagtgcc ttgatcgccg tgtcgtcgcg
     1021 cacatcgagc ttgcgcgctt cgaccggctt gccggcgagg ccgtcgatgc ggatatcggt
     1081 ggcgatcacg cgcgcgcctt cgcgcgcgaa cagttccgcg gtggcgagac caatgccttg
     1141 tcctgccgcg gtgatcaggg ccgtcttgcc ggccagtctt tgtgtcattt acgactccag
     1201 ttggatgggc ttcttttgtt tctctcgtct tcgtaacttg tctttgtaac gcgtcttcgt
     1261 cacgcgtctt cgtctcatca aagccgataa aacgcggcgg cgttgccgcc aaaaaccgct
     1321 tcgcgctcgt cgtcgctcaa cgacgcgagc aaggtgttcg ccaccgaatg ccacagcaga
     1381 tagtcgccgt tcagatcgag caccggccag tcgctgcccc acatcaaacg cgcgggaccg
     1441 aatgacgcca gcagatggtc gacatacggt tgcagcgttt cctcggtcca gccaggcgag
     1501 gcttcggtga cgagtcccga cagcttgcag tgcacttgcg gcaaccgggc aagccgcgtg
     1561 atcgcatcgg cccatgtttg ccaacccgcg cgaccgtagc gaatcggcgg cttcgcgccg
     1621 tgatcgacaa ccacgcgcag cgtgggaaaa cgcgccgcga acgtttcgaa aggctcgacg
     1681 tgccgtgcat aaatcaatgc gtcgaacgtc aggtcgtgcg cgatcagtgc ttcgatcgcg
     1741 ggcgcgaggt cggggttcgc aatccatgta tcgtcgggca aatcctgcag catcggtcgc
     1801 acgcctttga acttcggctc atgcgccagc gcttcgatca cagccggcgc gttcggcaac
     1861 agcaacggca cccagcccac cacgcccgca atcgagggtt cgttgcgcgc gatgtccagc
     1921 aagtaacgcg tttcgtcgat cgttggcgcc gcctgtacca ccaccgtccg ttcgatgccc
     1981 gctttttcgc gcaacggctt cagatcggcg ggaccgaacg gccgatagag gattttcagt
     2041 tccggcgtga gccactcgta gtcgccgcga gcggggtccc aatagtgctg gtgggcgtcg
     2101 ataggcatcg tcaaatcaat cctctggtgc gggcgcgcgg ctgtcgagca agcctttgtc
     2161 gcgcaacgcg gaccacaggt ccgcggggat cggctgctcg aacgacgcgg cgttctcgcg
     2221 caattcgtcg gcgctgcgcg cgccggtcaa cacggtggca accgccgaat gcgcatacgg
     2281 aaactgcaag gcggcggcgg caagcggtac gccatgcaca cggcacactg cttccagccg
     2341 cgcgacgcgc tcaatgactt cttgcggcgc gtcgccgtag ttgaatttca gatcgccttc
     2401 gacgccgcgc gccagaatgc cggaattgaa cgcgccacct aacaggatgc tgacgccgcg
     2461 ttcttcacat gctgggagca gatcgtcgag cgtggtctgt tcgaggagcg tataacggcc
     2521 cgcgagcaac gcgcaatcga tatcgaattc agccatcgcg tcgagtatga ccgcgccttc
     2581 attgacgccg agcccgaccg ccttaaccgc gcccgaggac cgcagatcat cgagcgcacg
     2641 aaagccgccg ccctcggtca attgccgcca gtagtggggg ttcttgtcgc cgtgcgtgaa
     2701 gcggccgata tcgtgcacca gaagaatgtc gatatcgacg atgccgagac gctgctggct
     2761 gtcttcgaac gaccgcagaa tgccgtcgtg ggtgtagtcg tagatcgcct ggaatggcag
     2821 cgggtcttgc cagccttcgc tgccatcgta gggcgtggtg cgcggtacga aacggcgccc
     2881 gactttggtg gacagcacgt attcactgcg cggatagtcg cgcaacgcat cgcccagacg
     2941 gtgctcggct ttcgtgtgac cgtaatgcgg cgcggtatcg aagtagcgca cgcctgtatc
     3001 ccacgcggcg gcaatggtcg cgtgggcctc ttcatcggac agatcgcggt agaggccgcc
     3061 caggggcgcc gtaccgaggc ccaatccggt gacttgtaat gtacggcggc cgatgccgcg
     3121 ccgctgttcg atcttcgaac cgcctgttgt cgtcattgac cacgtctcct gtgtcgacca
     3181 gagttagcgc tttagcgctt actctggtcc catgcaaagc ggtcgaccag agttagcgct
     3241 ttagcgccta ctccgatccc atgcacagcg gtcgaccagg gttagcgctt actctagtcc
     3301 cctcgcactt actccaatcc cacttacgtt tttaccacgc agcgccgaga ttcgcccgcc
     3361 gcgctaacgg tttgtctgcc aacaggatcg ggtttgtctg ccaacaggat cgcgcatgaa
     3421 acgctcaatg cggctcgatc gccacaaccc gatgcagcgg cgcagcgttc gacggcaaac
     3481 atgcggggcc gaccggttca aaactcttgt aagtcaggat gaattcctga tgaccgagcg
     3541 cttccgactt cgacgccgca ccgagcgaca ccgccaccgt ttgctcgaat acttcgcggc
     3601 cgacttcgtc gagcgtgccg cggccttcga gaatacggcc tgcatcgacg tccatatcgc
     3661 cggcgagatt gcggtacgtc gcgggattcg cgcacacctt gatgaccggc gaaatcgccg
     3721 agcccaccac cgacccgcgt ccggtcgtga acagaatcac atgcgcgcca caggcgatca
     3781 gttcgccgat ttccgcgttg tcgctgatgt tcggaaagcc gaagcgcggc tcgccgtccg
     3841 gcacgacgtc gagcagatag aggccaccgg tcggcggcac atcgccaggt ttgatgatgc
     3901 cgacaatcgg cgacgcaccg cttttcgcat acgcacctaa cgatttctct tcctgcgtgg
     3961 tcaggccgcc gtccgcattg ccgacggcaa aactgccatg cccaaggatc gagtagtagc
     4021 gcgccgcctt cgcgacacac gcaatgattt catcgccgag ttccggcctc gcggcgcgcg
     4081 ttttcatatg gaactcgcag cccaccagct cgccggtttc ttcgaagatg caggtcgcgc
     4141 ctgcgtcgat cagatgatcg aacgcgcggc ccacggccgg attcgcggta atgccgctgg
     4201 tgccgtccga gccgccgcaa atcgtgccga tcaccagttc gctcagcgcc atcggcactt
     4261 tttgttgccc ggcgagttgc ctgcgtgcgt cacgcaccca gtcgacgctg tattgaatcg
     4321 tgctgcgtgt gccgcctttt tcctgaatcg tcaggacttc aacgggacgc ccgctcgcgc
     4381 gtacgacgtc cacgaggtag tgcttgttca tgctctcgca accgagcgat acgaacagca
     4441 ccgcgcccac gtttggatgt gtcgtaaggc gctcgagcat cttctcggcg tacccattcg
     4501 gatagcaacc gggaaaaccg atcagatgca ccggcggctc gcgctcggcc gaaggatcgt
     4561 cgaacgcatc gagcggttcg cgaaactgcg tgacgatctc gcgtgcgacg tgatgcgcgc
     4621 actcgaccag atacgccacc gcaaccacgt tgcggatgcc tttgcggcca tcgctgcgca
     4681 gatagccttg cagcatgggc gaccgcgcgt cggcggatgc tatggaagtg tcagtcatgg
     4741 tcattccagc gcagcgcgag gccacgttat tgtgctcaca tcagtgatgg acgaactcgt
     4801 ggcccgcgtc gtgcgtatag gtgggcagat agtcgctttc gagattgtgc gtatgcagat
     4861 gcgcgccgcg cgccaccgct tcggtcacgt gaccgatcac cgcgccgtag cgcagcacct
     4921 tgtcgtctct cgcgagctca tggcgcgcca ccttgtggcc gagatcgatg gttctggtaa
     4981 gcgtcacgcg ttcgccttcg atctcgactt gcgtgcccgc ctcgagacgc gccgccgcga
     5041 tcagacagtt atccgtggga ctgagcaaaa tcaggcgtga gtcggttggc gatgtttggg
     5101 gcgtttgcaa tggacggctg gtcaagtgag ttctccgtgg tgcgtgcgca ttagttttga
     5161 ccttcgccac tggcgagccg cgccaccatc agcgagccga ggatgatcgc gccatagatc
     5221 gcctgaatcc agaacgacgg cacctgggca agcgtcagca ggttctgcac gacgccgagc
     5281 aacagcacgc cggagagcgc gccgaacatc gtgcccttgc cgccgtcgag tgaaatgccg
     5341 ccgatcaccg ccgccgcgaa caccgtgaaa atcatgccgt tgccatggtt cgcgttgatc
     5401 gcgccgacat agccggtcac gatcagaccg cccactgagg ccaggatgct gccgagcacg
     5461 aacacccccc acgtgatgcg ttcaacgcga atccccgccg cacgcgccgc ttccggattg
     5521 ccgccgattg catataacgc acggccgagg cggtgatagc gcagcacgaa tgcagcaatc
     5581 gcgaacgcgg ccgctgcgag ccacacggac aacggcaagc cgagcacgat ggtggtcgcg
     5641 agcgcgaaga acgacggcgg catatcgaac agcgtgccgc ctttggtcgc gccgaccagc
     5701 atgccgcgca acacgatcag catggccagc gtgacgatga acgcgttcag ccgcagacgc
     5761 accaccagga aaccgttgac gaagccgatc accgcgccaa ccacgatgat cgccagcaaa
     5821 cctgccgccg ccggccattg catcccgaag cccgcggacg ccgccggcat caccagcatc
     5881 gcgccgacag cgggcgcaat acccaccgtc gattcgagcg acaaatcgaa cttgcccgtc
     5941 aacacgatca acgattcggc gagcacgacc aacgccaggg ccgcggacgc acccagcacg
     6001 ctgaccagat ttgccttcgt cagaaagctc gggctgacaa acgcgccgat cacgatcagc
     6061 aacgcgagcg cgggcagcag agccagttca cgcagccgcg cgaactcact gcgagcaggt
     6121 ttgccgcgcg tcgcctgtgc agcggtctgc ggctgggcag gctgagcttg cgccgtgccg
     6181 aatgcagggc tgggcacact attcttcatg aagactgact ccttcaacgg acgcgatcag
     6241 gtcgtggtcc tgccaacccg cgggaaattc ggccgcgaca cggccacgga acatgaccag
     6301 cacgcggtca caggtgcgca gatcgtcgag ttcgccggac acgaccagca cggctttacc
     6361 gtcttcgcgc acacgatcca cgactgacag caacgcttct tttgatttca cgtcgacgcc
     6421 tgctgtcggg tcgatcagca cgagtatgtt gggatcggtg gcgagcgcgc gcgccatcac
     6481 gaccttctgc tgattgccgc ccgataggcc cgacacgata tgttctgggc cttgcgcaac
     6541 gatgccgagc gcgtcgatca tcttctgacc gaaggcatgc ttcttcgccg gtggcgcgat
     6601 gccgaatttg ccgagcaaac gcgcgatcgt catcgaggca ttttcggcaa ccgattgcgt
     6661 cagcaccagg ccttcgtgat ggcgatcctt cggcacgcag ccaatgccgt gcgccaatgc
     6721 tgccggcacg tcgccggggg gcaacctcat gccatccacg ctgatcgcgc cgctcttcgg
     6781 cgcgcgcaaa cccgcaatcg cttcggccac gctcgtgcgg ccgctgctcg ttgcaccggt
     6841 tagcccgacc acttcgccac gcttcaccgt gaacgacacg ccttcgtaat cgggaccggc
     6901 caggtctttg acatccagcg cgatggctgt gtccacagac aacgcgtcgc gcgccgccgc
     6961 atcggcgacg gcaaggccgc cacgttcgcc ggtcatcgct tcgatcaact gctcacgcgg
     7021 caacgcgcaa accggcgcgc tgacgatatg ccgcgcgtcg cgcagcaccg tcacggcctg
     7081 gcagatttca tagacctctt gcagatgatg cgagataaac aggaacgtca ccccttcgcg
     7141 ctgcaactcg ctgatgcg
//
LOCUS       DATASH010000552         4560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5630951,
            whole genome shotgun sequence.
ACCESSION   DATASH010000552 DATASH010000000
VERSION     DATASH010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4560
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5630951"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..1067
                     /locus_tag="VHV99_15230"
     CDS             147..1067
                     /locus_tag="VHV99_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3636810.1"
                     /translation="MNSLDHVDLNLLRVFQAIVEERSLTRAGQRLALSQPAISYSLGR
                     LRTLFDDPLFIRTRAGMQPTPIALELSGIVARALDTVREVLRYAEHFDPAVSTRTFRL
                     SLSDAGELAYLPPICAALHRQAPRVKLRIEPLPVEEIEDALRASRLDFAIGNLPTLTA
                     RTRHQPLFEETYVCMTRKRRGLPRTKELSMQAFLDASHVQITSVEHSHYALDDAFRAQ
                     GVGRHIALELPHFVALPSVLAVTDLFATLPRRLAQIFNRDGGFQLYALPVTLPMAAVT
                     MHWHEHFDQDEGNVWLRNLMADIVRRFDER"
     gene            complement(1089..1976)
                     /locus_tag="VHV99_15235"
     CDS             complement(1089..1976)
                     /locus_tag="VHV99_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018417807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3636811.1"
                     /translation="MELRQLRYFIAVAEEMNITRAANRLHMTQPPLSRQIQQIEESVG
                     LPLFERGARPLRLTEAGRIFYAQARRLVDDADELAPLTRRLAQLAERIVIGFVPSTLY
                     RALPAVIRAFREAAPHIELSLIEMFTIEQLAALKGGRIDVGFGRLRFDDAQLAREVLV
                     EERMIAALPQDHPLARQKKPLTLAALAQETLIVYPSTPRPSYADQQLSAMHDHALEPK
                     AIHEVRELQTALGLVAAQVGVCLVPESVEGLRAHGVVYRPVPAANIASPIIMSRRLQD
                     ESPATTLLCSLARELFKRA"
     gene            2079..3236
                     /gene="catB2"
                     /locus_tag="VHV99_15240"
     CDS             2079..3236
                     /gene="catB2"
                     /locus_tag="VHV99_15240"
                     /EC_number="5.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_030103333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muconate cycloisomerase CatB2"
                     /protein_id="HEX3636812.1"
                     /translation="MIATPVKIESVETILVDVPTIRPHRLSVATMNCQTLVLVRIRCA
                     DGVLGVGEGTTIGGLAYGEESPESIKVNIDTYFAPLLKGMDATRPGAAMAKLRALFQG
                     NRFARSAVETALFDAQAQRLGVPLSELFGGRVRDSVDVAWTLASGDTTRDIDEAERVF
                     EAKRHRVFKLKIGSRAVADDVAHVVAIQKALDGRGEVRVDVNQAWTESEAIWAGKRFA
                     DAGIALIEQPIAAENRAGLKRLTDLSQVPIMADEALHGPADAFAMASARAADVFAVKI
                     AQSGGLTGAAQVAAIAMAANIDLYGGTMLEGAVGTIASAQLFSTFGELKWGTELFGPL
                     LLTEEILTEPLRYENFALHLPEGPGLGITLDWDKIDRLRRDTRKGASVTTN"
     gene            3284..4192
                     /gene="catA"
                     /locus_tag="VHV99_15245"
     CDS             3284..4192
                     /gene="catA"
                     /locus_tag="VHV99_15245"
                     /EC_number="1.13.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004525033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018576 - catechol 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019615 - catechol catabolic process,
                     ortho-cleavage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catechol 1,2-dioxygenase"
                     /protein_id="HEX3636813.1"
                     /translation="MNKQAIDALLQKINDSATSEGNPRTKQVVNRIIRDLFYTIEDLD
                     VQPDEFWTALNYLGEAGRSGELGLLAAGLGFEHFLDLRLDEAEAKAGIEGGTPRTIEG
                     PLYVAGAPLSDGHARLDDGTDPGQTLVMRGRVLGEDGKPLANALVEVWHANHLGNYSY
                     FDKSQPAFNLRRSIRTDAEGKYSFRSVVPVGYSVPPQGQTQLLLDQLGRHGHRPAHIH
                     FFVSAPGCRKLTTQINIDGDPYLWDDFAFATRDGLVPAVRQAEGAEGKPYGVEGQFAL
                     IDFDFTLFKERDNLPGAEVERVRAEA"
     gene            4323..>4560
                     /gene="catC"
                     /locus_tag="VHV99_15250"
     CDS             4323..>4560
                     /gene="catC"
                     /locus_tag="VHV99_15250"
                     /EC_number="5.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011881489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016159 - muconolactone delta-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muconolactone Delta-isomerase"
                     /protein_id="HEX3636814.1"
                     /translation="MLFHVEMTVNLPIDMDAERAARLKSDEKAMSQKLQQEGVWRHLW
                     RIAGRYANISVFDVESPAHLHDVLSQLPLFPYMDV"
BASE COUNT          862 a         1354 c         1480 g          864 t
ORIGIN      
        1 ttgcgcgtcg acatagccgg acgcttgcag catggtccaa cggctttcgt aggtgggatg
       61 atcgatgaag caaagtctcg ctgagggact gatatgcgtc aaatcgatct aaaataaaat
      121 ataaaatcaa tttcatgaat atctcgatga attcgctaga ccacgtggac ctcaatctgc
      181 tgcgcgtttt ccaggcaatc gtcgaggagc gcagcctcac acgggccggg cagcggctcg
      241 cgctatcgca gccggcgatc agttactcgc tcgggcgttt gcgaactctt ttcgacgacc
      301 cgctgttcat tcgcacgcgc gccggcatgc agcccacgcc gatcgcactc gaactgtcag
      361 gcatcgttgc acgggcgctg gatacggtgc gtgaagtgct gcgctacgcg gagcacttcg
      421 atccggcggt gagcacgcgc acgttccgtt tatcgctgtc cgatgcgggc gagttggcct
      481 atttgccgcc gatttgcgcg gcgttgcacc ggcaggcgcc gcgggtgaaa ttgcgcatcg
      541 agccgttgcc ggtggaagaa atcgaagatg cgttacgcgc gagccggctg gattttgcaa
      601 tcggcaatct gcctacgctg accgcgcgca cgcgtcatca accgttgttc gaagaaactt
      661 acgtttgcat gacgcgcaaa cggcgcggct tgccacgcac gaaagagttg agcatgcagg
      721 cgtttctgga cgcctcgcat gtgcagatca cgtcggtcga gcatagccac tacgcgctcg
      781 acgatgcgtt tcgcgcgcaa ggcgtgggcc ggcatatcgc gctggaattg ccgcatttcg
      841 tggccttgcc gagcgtgctg gcggtgaccg acctgttcgc cacgctgcca aggcgtctcg
      901 cgcagatttt caatcgggac ggcggatttc agctttacgc gttgcctgtc acgttgccca
      961 tggcggcggt cacgatgcat tggcacgaac atttcgatca ggatgagggc aacgtgtggt
     1021 tgcgcaatct gatggccgat atcgtgcggc ggtttgacga gcgttgagag ggggcgctat
     1081 gggctgcatc aagcccgctt gaacaactcc cgcgccagcg aacacagcaa cgtcgtcgcg
     1141 ggcgactcgt cctgcaagcg tcggctcatg atgatcggcg acgcgatatt ggccgccgga
     1201 accggccggt acaccacgcc atgggcgcgc aaaccctcga cgctttccgg cacgagacat
     1261 acgcccactt gcgccgccac cagacccagc gcggtttgta attccctcac ttcatgaatc
     1321 gccttcggct ccagcgcgtg gtcgtgcatc gccgagagtt gctggtccgc ataactcggc
     1381 cgcggcgtgc tcggatagac gatcagcgtt tcctgcgcga gcgcggcgag cgtcagcggt
     1441 ttcttttgcc ttgcgagcgg atggtcttgc ggcagtgccg caatcatccg ttcttcgacg
     1501 agtacctcgc gcgccagttg cgcatcgtcg aaacgcaagc ggccgaagcc gacatcgata
     1561 cggccgcctt tgagggccgc gagttgctcg atcgtaaaca tttcgatcag cgatagctcg
     1621 atatggggcg cggcctcgcg aaaggcgcgg atcactgccg gcagcgcgcg gtagagcgtg
     1681 gacggcacga agccgatcac gatgcgctcg gccagttgcg cgaggcgtcg tgtgagcggg
     1741 gcgagttcgt cggcgtcgtc gaccaggcgc ctcgcctgcg cgtagaaaat ccggccggct
     1801 tcggtcagcc gcagcggccg cgcgccgcgt tcgaacagcg gcaagccgac gctttcttcg
     1861 atctgctgga tctggcggct gagcggcggc tgcgtcatat gcaggcgatt ggcggcccgc
     1921 gtgatgttca tttcttccgc gaccgcgatg aaatagcgga gttggcgaag ttccatgcat
     1981 acccctaagg tatggaagta gtcggaatcg gtgttggact gcttcgcggg ttgtttctac
     2041 tatggagccc tcgatcaagc agttcaggag agcaacgaat gatagcaaca cccgtgaaga
     2101 tcgaaagcgt ggagacgatt ctcgtcgacg tcccgacgat tcgtccccac cggctctcgg
     2161 tcgcaacgat gaactgccag acgctcgtgc tggtgcgtat ccgttgcgcg gacggcgtgc
     2221 tcggcgtggg cgagggcacg accatcggcg gcctcgcgta cggtgaggaa agcccggaaa
     2281 gtatcaaggt caacatcgac acgtacttcg cgcccctgct caaaggcatg gacgcgaccc
     2341 gcccgggcgc ggcgatggcg aagctgcgcg cgctgtttca gggcaaccgt tttgccagat
     2401 ccgcagttga aaccgccttg ttcgatgccc aggcgcagcg cctcggcgtg cccttgtcgg
     2461 aactgttcgg cggccgcgtg cgcgattcgg tggatgttgc atggaccctc gcgagcggcg
     2521 acacgacgcg cgatatcgac gaagccgagc gggtattcga ggcgaagcgg catcgcgtct
     2581 tcaagctgaa gatcggctcg cgcgcggtgg ccgacgacgt cgcgcatgtc gtggcgatcc
     2641 agaaagccct ggacgggcgc ggcgaagtac gggtcgacgt gaatcaggca tggaccgaat
     2701 ccgaagcgat ctgggccggc aagcgtttcg ccgacgcagg gattgcactg atcgagcagc
     2761 cgatcgccgc ggagaatcgc gcgggcctca aacgtctgac cgatctttcg caagtgccga
     2821 tcatggccga cgaagcctta catggcccgg ccgatgcctt cgccatggcc agcgcgcgtg
     2881 ccgccgatgt cttcgccgtc aagatcgcgc aatcaggcgg gttgaccggc gcggcccagg
     2941 tggcggcgat cgcgatggcc gcgaacatcg acctgtacgg cggcacgatg ctcgaaggcg
     3001 cggtcggcac gatcgcctcg gcgcaactct tcagcacgtt cggcgaactg aagtggggca
     3061 ccgaactgtt cgggccgttg ctgctgactg aagagattct taccgagccg ctgcgttacg
     3121 agaattttgc gttgcatctt ccagaagggc cgggtctcgg catcacgctc gactgggaca
     3181 agatcgaccg cttgcggcgc gatacgagga agggcgcaag cgtcaccacg aactgacggt
     3241 tcatccgcta gccgccaagc acattaaacg aaggagatac cccatgaaca agcaagccat
     3301 cgacgcactg ctgcaaaaga tcaacgacag cgccaccagt gaaggtaatc cgcgcaccaa
     3361 gcaagtcgtc aaccgcatca ttcgcgacct gttctatacg atcgaagacc tcgacgtgca
     3421 gccggacgaa ttctggaccg cgctgaacta cctcggtgaa gcgggccgga gcggcgagct
     3481 cggtctgttg gccgcgggtc tcggcttcga gcattttctc gatctgcgtc tggacgaagc
     3541 ggaagcgaaa gccggtatcg aaggcggcac gccgcgcacc atcgaaggtc cgttgtatgt
     3601 ggcgggcgcg ccgctgtcgg acggccatgc acgtctggat gacggcaccg accccggtca
     3661 gacgctggtg atgcgcggcc gcgtgctcgg cgaagacggc aagccgttgg ccaatgcgct
     3721 ggttgaagtg tggcacgcga accacctcgg caattactcg tacttcgaca agtcgcagcc
     3781 ggccttcaat ctgcgccgct cgattcgtac ggatgccgaa ggcaagtaca gcttccgcag
     3841 cgtggtgccg gtgggttact cggtgccgcc gcaagggcaa acgcagttgt tgctcgatca
     3901 gttgggccgt catgggcatc gtccggcaca tatccacttc tttgtttcgg cgccgggctg
     3961 ccgcaagctg accacgcaga tcaatatcga cggcgatccg tatctgtggg acgactttgc
     4021 gttcgccacg cgcgatggcc tcgtgccggc cgtcaggcag gccgagggtg cggaaggcaa
     4081 gccgtatggc gtggaaggcc agttcgcgtt gatcgacttc gacttcacgc tgttcaagga
     4141 acgcgacaac ctgccgggcg ctgaagtgga gcgcgtgcgc gccgaagcct gaggttcacc
     4201 gtcagacgcg tcgacgcctg aaaggcgtgg acgtatcgca tgaaatctat cggccctggc
     4261 tgcgcggcat ggtttgccgg gcagccaggg ttcttttatg accgatcaga aggagagcaa
     4321 gcatgctgtt tcatgttgag atgaccgtca atctgccgat cgatatggac gccgagcgcg
     4381 ccgcgcgcct gaagtccgat gaaaaggcga tgtcacagaa gctgcagcag gagggggtat
     4441 ggcggcacct gtggcgcatc gccgggcgct acgcgaatat cagcgtgttc gatgtggaaa
     4501 gccccgcgca tctgcatgac gtgctgagcc agttgccgct gtttccgtat atggatgttg
//
LOCUS       DATASH010000553         1672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_90465,
            whole genome shotgun sequence.
ACCESSION   DATASH010000553 DATASH010000000
VERSION     DATASH010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90465"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..1578
                     /locus_tag="VHV99_15255"
     CDS             142..1578
                     /locus_tag="VHV99_15255"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX3636815.1"
                     /translation="MTKSRQRDSLSPNKLRWIRMALGRQRDRQSEMLVTWSELPRSPG
                     HVFYDRLQAILIAAGFDGFVEALCANHYSVVRGRPSLPPGRYFRMLLIGYFEGIDSER
                     GLEWRCADSLSLREFLRLAERETVPDHSWLSRTRSRLPLELHEQVFTWALHRLSEHGL
                     IKGERIGVDASTMEANAALRSIVRRDSGEGYREMLTRMAKESGIATPTADDLVRLDRA
                     RTGKKLSNADWESPIDPDAKIAKLKDGRTRLAYKPEHALDLDTGAIIAAELHGADQGD
                     TVSLSVTLEAAMEQLAALDAAPTPEAPAALITDKGYHSRDILKRLADGPWKTRVSERR
                     VDGVLRWHGDHAARRAVYNNRARLLSGVAREAFRLRAEWVERSFALILDRGGMRRAWL
                     RGRANIHKRYLIHVAGYNLGLIMRLLTGAGTPRELHARGSAYLFYVILPDAALLAIIA
                     FADEHPIAVLFTFEAAPFNLTPAFSTGC"
BASE COUNT          303 a          504 c          546 g          319 t
ORIGIN      
        1 gggattgaac ctatagtcgc aatgtggagc cgcttttttg gggggacttg actctgaagt
       61 taaagccaca cggcgacgga acagataaaa agatggttgt tgattggatt ataaagccag
      121 acgcgccccc ttagcagccc gttgacgaaa tcgcggcagc gcgattcgtt gtcaccgaac
      181 aagttgcgtt ggattcggat ggcattaggt cggcagcggg atcgtcaatc ggagatgctg
      241 gtgacgtggt cagaactgcc gcgctcgcct ggccacgtgt tctatgaccg gctgcaggcg
      301 atcctgatcg cggcggggtt tgacggcttt gtggaagcgc tgtgcgccaa ccactactcg
      361 gttgtgcgcg gacgaccgtc actgccgccc ggccgctact tccgcatgct gctgatcggt
      421 tatttcgaag gcatcgacag cgaacgcggc ctggaatggc gctgtgccga cagcctctcg
      481 ttgcgcgagt tcctgcggct cgccgagcgc gagactgtgc cggatcattc ctggttgagc
      541 cgcacgcgct cgcgtctgcc gctggaactg cacgagcagg tgttcacctg ggcgctgcac
      601 cgcctgtccg agcacggtct gatcaagggc gagcggatcg gtgtggacgc ctcgacgatg
      661 gaggcgaacg ccgcgctgcg cagcatcgtg cgacgcgata gcggcgaagg ctatcgcgag
      721 atgctgaccc gcatggccaa ggaaagcggc atcgcgacac cgacggccga tgatctggtg
      781 cggctggatc gcgcacgcac gggcaagaag ctgtcgaacg ccgactggga aagcccgatc
      841 gatccggacg ctaagatcgc caagctgaaa gatggccgca cccgtctggc ctacaagccc
      901 gagcatgcgt tggaccttga taccggtgcg atcatcgcgg ccgaattgca cggagccgac
      961 cagggcgaca ctgtcagcct gtcggtcacg ctcgaagccg cgatggagca actggccgcg
     1021 cttgatgcgg cacccacgcc agaggcgccg gcggcgttga tcactgataa gggctatcac
     1081 tcccgcgaca tactgaagcg cctggcggac ggaccatgga agacgcgcgt gtcggagcga
     1141 cgggtcgacg gagttctgcg ctggcacggc gatcatgccg cgcggcgggc ggtctataac
     1201 aatcgcgcac gcctgctgtc cggcgtggcg cgcgaggcct tccggctgcg tgccgaatgg
     1261 gtggagcgca gcttcgcttt gatccttgat cgtggtggca tgcgacgcgc ctggctgcgc
     1321 ggccgcgcca acatccacaa gcgctatctg atccacgtcg caggttacaa tctcgggttg
     1381 atcatgcgcc tgctcaccgg agccggaacc ccgcgcgagc tccatgcacg ggggtccgcc
     1441 tatctgttct acgtcatcct gccggatgct gcgcttctcg ccatcattgc gttcgccgac
     1501 gagcatccga ttgctgttct cttcaccttc gaagccgcac cctttaatct aacaccggct
     1561 ttctcaacgg gctgctaagc gtgagccgac cagcacggtt gacgttcgtg actgtcggct
     1621 ctgcgatctg tctggtggcc gggagatcgg gggtgaacat tgataccgat ag
//
LOCUS       DATASH010000554         2182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_486393,
            whole genome shotgun sequence.
ACCESSION   DATASH010000554 DATASH010000000
VERSION     DATASH010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486393"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1155
                     /locus_tag="VHV99_15260"
     CDS             <1..1155
                     /locus_tag="VHV99_15260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016355339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative maltokinase"
                     /protein_id="HEX3636816.1"
                     /translation="LVIGVQVVLKLVRRLVGGIHPEAEMSRHLTKLGYQNTGPLFGEV
                     VRVDPEGVPHTLCILQGFIDNQGDAWNYALDYLRRTVDELALAVDTDDHSLDREKEMQ
                     GIEGYRKVTGIIGQRLGELHVALAQPSDDPAFAPEKASKKDVRGWNDGTHALLVTALD
                     ILAAKLLEFSEHDRAMAQSLLDRRDLLLAAVQKLVPENANALRIRIHGDFHLGQVLMA
                     QGDAFLIDFEGEPARPLEERRKKSSPLRDVAGLLRSLSYASAAAQPTTGNAPQATAYR
                     KRALFERLRGHGEEAFLIDYLAAVAASAQPVAGDEATQAALLDLFLIEKAAYEIRYEA
                     ANRPTWIGLPLRGLASLASRLLGDTGAPPAPGSRPVDPAFKDTSDGNENE"
     gene            1148..>2182
                     /locus_tag="VHV99_15265"
     CDS             1148..>2182
                     /locus_tag="VHV99_15265"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403679.1"
                     /note="catalyzes the transfer of a segment of a
                     1,4-alpha-D-glucan chain to a primary hydroxy group in a
                     similar glucan chain; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HEX3636817.1"
                     /translation="MNKRTDDRLLTEGLHPLDIDALVEARHPDPFSKLGLHQTDAGPV
                     VRVLLPGATGVQVLERDSSKPLGTLTQVHPAGLFIGFVDEMQAYRLNIDWHGTPQEIA
                     DTYSFGPVLDDDALNRISWGDPYAVMECLGSRPIVHGGVAGVRFAVWAPNARRVSVVG
                     DFNSWDGRRHPMRLRHNAGVWELFVPGLGAGTRYKYEIISRDGHALPLKADPCAMQSE
                     RPPATASVVADAEAIDGYAWTDSEWMNSRGPRQTVNAPISVYEVHAESWLRVPEEGNR
                     GMNWHELAERLIPYAKSMGFTHLEFMPIAEHPFGGSWGYQPLGQFAPSARFGTPEQFA
                     EFVNRAHEAEL"
BASE COUNT          397 a          640 c          726 g          419 t
ORIGIN      
        1 ctcgtgatcg gggttcaggt cgttttgaaa ctggtgcgcc ggctggtcgg cgggattcat
       61 ccggaagccg agatgagccg gcacctgacg aagctcggtt accagaatac ggggccgctg
      121 ttcggtgaag tggtacgcgt cgatccggaa ggcgtgccgc atacgctgtg catcttgcaa
      181 ggctttatcg ataaccaggg cgatgcgtgg aactacgcgc tcgattacct gcgtcgtacc
      241 gtcgatgaac ttgcgctggc cgtggatacc gacgaccact cgttggaccg cgagaaggaa
      301 atgcagggca tcgaaggcta caggaaagtc acggggatca tcgggcaacg gttgggcgaa
      361 ctgcacgtcg cgctcgcgca accgtccgac gaccctgctt ttgcgcccga aaaggcgtcg
      421 aagaaagacg tgcgcggctg gaacgacggc actcacgcgc tgctcgtgac ggcgctcgac
      481 atcctcgcgg cgaagctcct ggaattcagc gagcatgacc gcgccatggc gcagagcctg
      541 ctcgatcgtc gcgacttgct gctggcagcc gtgcaaaagc tcgtgccgga aaacgccaat
      601 gcattgcgca tccgtattca cggcgatttt cacctcggac aggtgctgat ggcgcagggc
      661 gacgcgttcc tgatcgactt tgaaggtgaa ccggcgcggc cgctggaaga acggcgcaaa
      721 aaatcgagtc cgttgcggga tgtggccggc ttattgcgtt ccctgtcgta tgcgagcgct
      781 gccgcgcagc cgacgaccgg aaacgccccg caggcaaccg cctatcgcaa gcgcgcgttg
      841 ttcgagcgcc tgcgcggtca tggcgaggaa gcgttcctga tcgactacct cgcggcggtg
      901 gcggcttctg cgcaaccggt tgccggcgac gaagccacgc aagccgccct gctcgatctt
      961 ttcctgatcg agaaggccgc ctacgaaatc cgctatgaag cggcgaaccg tccgacctgg
     1021 atcggcttgc cgttgcgtgg cctcgcgtcg cttgcgagcc gtttgcttgg cgatacgggc
     1081 gcgccgcccg cgccgggttc gcggcccgtg gaccccgcat tcaaggacac atccgatgga
     1141 aacgaaaatg aataagcgca cagacgacag gctgctgacc gaaggtctgc atccgctcga
     1201 tatcgacgcg ctcgttgaag ctcgccatcc cgatccgttt tcgaagctcg ggcttcatca
     1261 gaccgatgcc ggccccgtcg ttcgcgtgct gttgcccggc gcaaccggcg tgcaggtgct
     1321 tgagcgcgac agcagcaagc cgcttggtac gctcacgcag gttcatccgg cggggttgtt
     1381 catcgggttt gtcgatgaga tgcaggcgta tcggctgaac attgattggc acggcacgcc
     1441 gcaggaaatc gccgatacgt attcgttcgg ccccgtatta gacgatgacg cgttgaaccg
     1501 catttcctgg ggtgatccgt atgcggtgat ggaatgcctc ggctcgcggc caatcgtgca
     1561 tggcggcgtg gccggtgtgc gttttgcggt atgggcgccg aatgcgcgac gcgtttcagt
     1621 ggtcggcgac ttcaactcgt gggacggccg ccggcatccc atgcgcctgc gccataacgc
     1681 cggcgtctgg gaactgttcg tgccgggact tggcgcgggc acgcgctaca agtacgagat
     1741 catctcgcgc gacggccacg cgctgccgtt gaaagctgac ccgtgcgcga tgcaaagcga
     1801 gcggccgccc gcgacggctt cggtggtggc cgacgctgag gctatcgatg gatacgcctg
     1861 gaccgacagt gagtggatga attcacgcgg accgaggcag accgtgaatg cgccgatttc
     1921 cgtgtacgaa gttcatgcgg aatcgtggct gcgcgtgcct gaggaaggca accgcggcat
     1981 gaactggcat gagcttgccg agcgcctgat tccgtatgcg aagagcatgg gtttcacgca
     2041 ccttgagttc atgccgattg cggagcatcc gtttggtggt tcgtggggct atcagccgtt
     2101 ggggcaattt gcgccatcgg cgcggtttgg gacacctgaa caatttgccg agtttgtgaa
     2161 ccgtgcgcat gaagcggaac tg
//
LOCUS       DATASH010000555         2588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5233316,
            whole genome shotgun sequence.
ACCESSION   DATASH010000555 DATASH010000000
VERSION     DATASH010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5233316"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="VHV99_15270"
     CDS             <1..493
                     /locus_tag="VHV99_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3636818.1"
                     /translation="AKALSHEPRVLFLDEPTAGVDVELRRDMWKLVRSLAASGVTIIL
                     TTHYIDEAEEMADRIGVINAGEIMLVEEKTELMRKLGKKQLTLQLESPLAQVPPALGA
                     YGLDVAKGGNELIYTYEGDGGRTDIIALLKALDDAGIRFKDLHTTQSSLEDIFVSLLR
                     GNQ"
     gene            490..1251
                     /locus_tag="VHV99_15275"
     CDS             490..1251
                     /locus_tag="VHV99_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3636819.1"
                     /translation="MNIYAIRAIYKFEMARTWRTLMQSIIAPVISTSLYFVVFGAAIG
                     SRIKEVDGISYGAFIVPGLIMLSLLSQSISNASFGIYFPRFTGTIYELLSAPVSYLEI
                     VVSYVGAAATKSILLGLIILATAGLFVPLQVQHPFWMILFLVLTAVTFSLLGFIIGIW
                     ADSFEKLQLVPLLIITPLTFLGGSFYAVNMLPPFWKVVTLFNPIVYLVSGFRWSFYGL
                     ADVDVAVSLGMTALFLAVFLVIVAWIFKTGYRLKS"
     gene            complement(1408..2409)
                     /locus_tag="VHV99_15280"
     CDS             complement(1408..2409)
                     /locus_tag="VHV99_15280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer binding domain-containing protein"
                     /protein_id="HEX3636820.1"
                     /translation="MEHECDTLPHWLTGSPQGEAGHASAAYNSAVAAQPAYPAGTRQP
                     EKSAHAAEASRAPIRTASGIVPAGFLTLFTEEEIGTPSPAARRATPVISPLVRFGSAQ
                     DRIEFVRQRIKQLGFDSFSYSATRTTAHHKTMFVLTSYESQTWLTRYFRERYFELDPR
                     VALASPTGMPFLWNTADMRADLPRAQMRSERLGGLIDMLEATGRKSGILTQMPLPEPE
                     LSASLCFNSEIGNPRWMTESIVAETLMFAHTIHEFIWTHAKSVIGIAPAQQQRVTLSE
                     LQHAVLKAVVQGQRDKEIAYFLGLSPHNVDYHLRRLRQLFNVRNRVQLINVAQGYVT"
BASE COUNT          453 a          757 c          806 g          572 t
ORIGIN      
        1 cgcgaaggcg ctctcgcacg aaccgcgcgt gctgtttctc gacgaaccca cggcgggtgt
       61 ggacgtcgaa ttgcgtcgcg acatgtggaa gctggtgcgc tcgctagccg cgagcggcgt
      121 gacgatcatc ctcaccacgc actacatcga cgaagccgag gagatggctg atcgcatcgg
      181 tgtgatcaat gcgggcgaga tcatgctggt ggaagagaag acagagttga tgcgcaaact
      241 cggcaagaag cagttgacgc tgcaactgga aagtccgctc gcccaggtgc cgccagcgct
      301 gggtgcttat gggctggatg tcgccaaggg tggcaacgag ttgatctata cgtacgaggg
      361 cgatggcgga cgcaccgaca tcatcgcgct gctcaaggcg ctcgacgatg ccggcatccg
      421 cttcaaggat ctgcatacga cgcaaagttc gctcgaagac atcttcgtca gtctgctccg
      481 gggtaaccaa tgaatatcta cgcaattcgc gcgatctaca agttcgagat ggcacgcacc
      541 tggcgcacgc tgatgcagag catcatcgcg ccggtgattt cgacatcgct ttacttcgtc
      601 gtgttcggcg cggctatcgg ctcgcgaatc aaggaagtgg acggcattag ttacggcgcg
      661 ttcatcgtgc cgggtttgat catgctgtca ctgctgtcgc aaagcatttc gaatgcatcg
      721 ttcgggattt actttccgcg ctttaccggc acgatctatg agttgctttc ggcaccggtg
      781 tcgtatctcg agatcgtcgt gagctatgtg ggcgcggcgg ccaccaaatc gatcctgctc
      841 ggcttgatca ttctggctac cgctggcctg ttcgtgccgc tgcaggtgca gcatccgttc
      901 tggatgattc tgtttctcgt gctgaccgcg gtcacgttca gtctgctcgg tttcattatc
      961 ggcatctggg cggatagctt cgagaaattg cagctcgtgc cgctcctgat catcacgccg
     1021 ctcacgttcc tcggcggaag tttctatgcg gtcaatatgc tgccgccgtt ctggaaggtc
     1081 gtcacgctgt tcaatccgat tgtttatctg gtcagcggat ttcgctggag cttctatggc
     1141 ctagccgatg tggatgtcgc tgtcagcctc gggatgaccg cgctgtttct ggcggtgttt
     1201 ctggtgatcg tcgcgtggat cttcaagacc gggtatcggc tcaagagcta acggttttgc
     1261 gcaaacttgc tggcgcttgc cgtctcggct agctcgtccg gcgcggcgcg ctcgttcaag
     1321 gcgcgatacc tgagctcaat acgatgctcg gcgcgtctac ggccggctcg gcgcgtgcgc
     1381 cggccgcagc tcgctttcat gccgttccta cgtcacatac ccctgcgcca cattgatcaa
     1441 ctgcacgcga ttgcgcacgt tgaacaattg ccgcaggcgc cgcaagtgat agtcgacgtt
     1501 atgcggcgac agcccgagga agtaggcgat ttccttgtca cgctgcccct gcacgaccgc
     1561 cttcaacacc gcatgctgca actcgctcaa cgtgacgcgc tgctgctggg cgggcgcgat
     1621 cccgatcacg cttttcgcgt gcgtccagat gaattcgtgg atcgtgtgcg cgaacatcag
     1681 cgtttcggcc acgatcgatt cggtcatcca gcgcgggttg ccgatctccg agttgaagca
     1741 caggctcgcg ctcagttccg gctcgggcag cggcatctgc gtgagaatcc cgctcttgcg
     1801 gccggtggcc tccagcatgt cgatcaggcc gccgagccgc tcgctgcgca tctgcgcgcg
     1861 tggcaggtcg gcgcgcatgt cggcggtgtt ccagaggaac ggcatgccgg tcggcgaggc
     1921 cagcgcgacg cgcggatcaa gctcgaaata acgctcgcgg aaatagcgtg tgagccatgt
     1981 ctgcgactcg tagctggtca gcacgaacat cgtcttgtga tgcgccgtgg tgcgcgtggc
     2041 tgaatagctg aatgagtcga agccgagttg cttgatgcgc tgccgcacga attcgatgcg
     2101 gtcctgcgca ctgccaaatc gcaccagcgg actgatcacc ggcgtggcgc ggcgcgcggc
     2161 gggcgagggc gtgccgatct cctcttcggt aaagagcgtg aggaaacctg cgggcacgat
     2221 ccctgatgcg gtgcggatgg gtgccctgga tgcttcagcc gcgtgggccg atttctcggg
     2281 ttgccgcgtg ccggccgggt aagccggttg ggcggcaacc gccgagttgt atgctgccga
     2341 cgcatgcccg gcctcacctt gcggcgagcc ggtcagccaa tgcggcagcg tatcgcattc
     2401 gtgttccatg aaacggtagc tccggcgttc gaaacgcatg aaagacgaca acctgaatga
     2461 tcagctactt ccgaaaatta cggtagccgg ttccttgcta tcatttttga attcttgctc
     2521 tgacagcgga ctggcaacgc gggtatgaca gggttcactc ttcatacccg cgttgccagt
     2581 ccgctgtc
//
LOCUS       DATASH010000556         2161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6619090,
            whole genome shotgun sequence.
ACCESSION   DATASH010000556 DATASH010000000
VERSION     DATASH010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6619090"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /locus_tag="VHV99_15285"
     CDS             <1..1122
                     /locus_tag="VHV99_15285"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX3636821.1"
                     /translation="VSILGGVAYGQQLMLLAKNPEILVATPGRLIDHLERGRIDLSQL
                     QILVLDEADRMLDMGFIEDIETIVAATPATRQTMLFSATLDGKISSLTGRLLKDPERI
                     EIVQRLEQRTNIAQTVHYVDDRDHKDRLLDHLLRDSGLDQAIVFTATKMDADQLAGRL
                     ADAGFESAALHGDLPQGARNRTIKALRERRVRVLVATDVAARGIDIPGITHVFNYDLP
                     KFAEDYVHRIGRTGRAGRSGIAVSLVHHAEQGALKRIERFVRTPLPVNVVEGFEPRKS
                     APSGNGRPGFGGRGRPGGGNGGGRRFGSGSGTGKPAGNGGGSRSGSGNGGGWAGKSAG
                     GGSREGGFGGGSREGYGGSRDGGYGARRSDGPRTARRGS"
     gene            1569..>2161
                     /gene="aceA"
                     /locus_tag="VHV99_15290"
     CDS             1569..>2161
                     /gene="aceA"
                     /locus_tag="VHV99_15290"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009931004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HEX3636822.1"
                     /translation="MSREQQARQLQQQWETDPRWKGVKRTYTAEDVIRLRGSLQVEHT
                     LAKRGAEKLWESVNNEPFVNSLGALTGNQAMQQVKAGLKAIYLSGWQVAGDANVAGEM
                     YPDQSLYPANSVPLVVKRINNTLTRADQIQWSEGKNPGDEGYIDYFAPIVADAEAGFG
                     GVLNAFELMKAMIEAGAAGVHFEDQLASVKKCGHMGG"
BASE COUNT          460 a          641 c          670 g          390 t
ORIGIN      
        1 gtcagcattc tgggcggcgt cgcttacggt caacaattga tgctgctggc aaagaacccg
       61 gaaattctgg ttgccacccc gggccgtttg atcgaccacc tggaacgcgg ccgtatcgat
      121 ctgtcgcaac tgcagattct cgtgctcgac gaagctgacc gcatgctgga catgggcttc
      181 atcgaagata tcgaaaccat cgtcgcggct acgccggcaa cgcgccagac catgctgttc
      241 tcggccacgc tcgacggcaa gatcagctcg ctgaccggcc gcctgctgaa ggatccggaa
      301 cgtatcgaga tcgttcaacg cctcgaacag cgcaccaaca tcgcgcaaac cgtccattac
      361 gttgacgacc gcgatcacaa ggatcgtctg ctcgaccatc tgctgcgtga ttctggcctc
      421 gaccaggcta tcgtcttcac ggcaaccaag atggacgcgg accaactggc tggccgtctg
      481 gccgacgccg gtttcgaatc ggcagcgctc cacggcgatc tgccgcaggg cgcacgtaac
      541 cgcacgatca aggctctgcg cgagcgccgt gtgcgcgtgc tggtggcaac ggacgtcgcc
      601 gctcgcggta tcgacatccc gggcatcacg cacgtgttca actacgatct gccgaagttc
      661 gctgaagact acgtgcaccg tatcggccgt accggccgtg ctggccgctc gggtatcgcc
      721 gtgagcctcg tgcatcacgc ggaacaaggc gcgctgaagc gcatcgagcg cttcgtgcgc
      781 acgccgctgc cggtcaatgt cgtcgaaggc ttcgagccgc gcaagtcggc tccgtcgggt
      841 aatggccgtc ctggctttgg cggccgtggc cgtcctggcg gtggcaacgg cggtggccgt
      901 cgcttcggta gcggcagcgg cactggcaag ccggccggta atggcggcgg ttcgcgcagc
      961 ggtagcggca atggcggcgg ctgggccggc aagtcggccg gcggcggttc gcgtgaaggc
     1021 ggcttcggtg gcggttcgcg cgaaggctac ggcggttcgc gagacggcgg ctacggcgca
     1081 cgccgcagcg acggtccgcg tacagcgcgc cgtggtagct aagcagcgac tcagtcgtta
     1141 ccaggcatgc gctgtttgcg tagctcgttc agtacgtgag gcggcctgaa aagacccaga
     1201 cggccctgtg ccgtccgtcg gtcgaaagaa accggtgctc aggcgccggt tttttttcgc
     1261 ccatattttg gtaatcaccc atatttcacg atgtggaaaa tttttttgag tcgtaaaaaa
     1321 tcgtgctgca aggcacaaga gcgtctattg caagatggaa aaagacgttt cttaatgcgg
     1381 aacgcgacac ataaacaatt gatttttaac gaaaataaat aatcaaaaaa cgctcctaga
     1441 ccgctattgc acgccggtcg atttgcctag actcttatat aagacttcac gaaacgaaac
     1501 gcctcaaatg gcccgcttcg acgaagacaa aagtcaccca tccattacag gcaaccaagg
     1561 agcacaccat gtcccgcgaa caacaagcca ggcaactcca acagcaatgg gaaaccgatc
     1621 cacgctggaa aggcgtgaag cgtacgtaca ccgctgaaga cgtgatccgt ctgcgcggct
     1681 cgctgcaagt ggagcacacg cttgccaagc gcggcgcgga aaaactgtgg gaaagcgtca
     1741 acaatgagcc gttcgtcaac tcgctcggcg cattgacggg caatcaggcg atgcagcagg
     1801 tcaaggccgg cctgaaggcg atctacctgt cgggctggca agtggcgggc gatgcgaacg
     1861 tcgccggtga aatgtacccg gaccagtcgc tgtatccggc gaactcggtg ccgctcgtcg
     1921 tgaagcgcat caacaatacg ctgacgcgcg ctgaccagat ccagtggtcg gaaggcaaga
     1981 atccgggcga cgaaggttac atcgactact tcgcgccgat cgtggccgac gctgaagccg
     2041 gtttcggcgg tgtgctgaac gcgttcgaac tgatgaaggc gatgatcgaa gcgggcgccg
     2101 cgggcgtgca cttcgaagac cagctcgctt cggtgaagaa gtgcggccac atgggcggca
     2161 a
//
LOCUS       DATASH010000557         1730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2268870,
            whole genome shotgun sequence.
ACCESSION   DATASH010000557 DATASH010000000
VERSION     DATASH010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2268870"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..>1730
                     /gene="gcl"
                     /locus_tag="VHV99_15295"
     CDS             165..>1730
                     /gene="gcl"
                     /locus_tag="VHV99_15295"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014896848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HEX3636823.1"
                     /translation="MAKMRAVDAAVLVLEKEGINTAFGVPGAAINPFYSAMRKAGSIS
                     HVLARHVEGASHMAEGYTRAQPGNIGVCIGTSGPAGTDMITGLYSAQADSIPILAITG
                     QAQRARLYKEDFQAVDIESIAKPVTKWAVTVREPALVPRVFQQAFHLMRSGRPGPVLV
                     DLPIDVQLAEIEFDIDTYEPLPVYKPKATRKQIEAALTMLNNSDKPLIVSGGGVLNAA
                     AEDLLVEFAETIGVPVIPTLMSWGAIPDDHPLMAGMVGLQTSHRYGNATMLASDFVLG
                     IGNRWANRHTGSIEVYTKGRKFVHVDIEPTQIGRVFGPDLGIVSDAKAALELFVEVAK
                     EWKAAGKLKDRSAWVADCQQRKRTMHRKTHFDNVPMKPQRVYEEMNQVFGRDTCYVST
                     IGLSQIAGAQFLHVYKARNWINCGQAGPLGWTIPAALGVRAADPGRKIVALSGDYDFQ
                     FMIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRGFDMDYCVQLGFENINSPEMNGYG
                     VDHVAVAEGLGCKA"
BASE COUNT          351 a          548 c          516 g          315 t
ORIGIN      
        1 cacccgccat tccatacttt ttgtttcgaa ataagcgacc cggactgatc ttatcaaacc
       61 tttaggtgaa gcctacagtt tgatcaacac ctctggtttg catatcccgt ccgcgaattc
      121 gtcagccaac ccattcgccc aacacagaca ttcaggagac attcatggcc aagatgagag
      181 ccgtcgacgc agccgtgctg gtgctcgaaa aagaaggcat caacaccgca ttcggcgttc
      241 cgggcgcagc aatcaacccg ttctactcgg ccatgcgcaa ggcaggcagc atcagccacg
      301 tgctggcgcg tcacgtcgaa ggcgcgtcgc acatggccga aggctatacg cgcgcccagc
      361 cgggcaacat cggcgtgtgt atcggcacct cgggccccgc gggcaccgac atgatcaccg
      421 gtttgtactc cgctcaggcc gactcgattc ctattctggc tattactggc caggcgcagc
      481 gtgcgcgtct gtacaaggaa gacttccagg ccgtcgatat cgaatcgatc gccaagcccg
      541 tcaccaaatg ggccgtcacc gtgcgtgaac cggccttggt gccgcgcgtg tttcaacagg
      601 catttcatct gatgcgctct ggccgtccgg gtccggtgct ggtcgacctg ccgatcgacg
      661 tgcagctcgc cgaaatcgaa ttcgacatcg acacgtacga accgctgccg gtctacaagc
      721 cgaaagcgac gcgcaaacag atcgaagccg cgctcacgat gttgaacaac tcggacaagc
      781 cgctgatcgt ttcgggtggc ggcgtgctga acgcagccgc tgaagacctg ctggtcgaat
      841 tcgccgaaac gatcggcgtg ccggtgatcc cgacactgat gtcgtggggc gcgattcccg
      901 acgaccatcc gctgatggcc ggcatggtcg gcttgcaaac ctcgcaccgc tacggcaacg
      961 cgaccatgct cgcgtccgac ttcgtgctcg gcatcggcaa ccgttgggcg aaccgtcaca
     1021 cgggcagcat cgaggtttat acgaagggcc gtaagttcgt gcatgtggat atcgagccga
     1081 cgcaaatcgg tcgcgtgttc ggtccggacc tcggcatcgt gtcggacgcg aaagccgcgc
     1141 tcgaactgtt cgttgaagtg gcgaaggaat ggaaggccgc cggcaagctg aaagaccgca
     1201 gcgcatgggt cgccgattgc cagcagcgca agcggacgat gcatcgcaag acgcacttcg
     1261 acaacgtgcc gatgaagccg cagcgcgtct acgaagagat gaaccaggtg ttcggccgcg
     1321 atacgtgcta cgtcagcacg atcggtttgt cgcagatcgc cggcgcgcaa ttcctgcacg
     1381 tctataaggc gcgcaactgg atcaactgcg gccaggccgg cccgctcggc tggaccattc
     1441 cggccgcgct cggcgtgcgc gccgccgacc cgggtcgcaa gatcgtcgcg ctgtcgggcg
     1501 actacgactt ccagttcatg atcgaggagc tcgccgtcgg cgcgcagttc aacatcccct
     1561 acatccacgt cctggtcaac aatgcctacc tcggcctgat ccggcagtcg cagcgggggt
     1621 tcgacatgga ctactgcgtg cagctcggct tcgagaacat caactcgccg gagatgaacg
     1681 gctacggcgt cgatcacgtc gcggtggccg aaggtcttgg ctgcaaggcg
//
LOCUS       DATASH010000558         5330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_683934,
            whole genome shotgun sequence.
ACCESSION   DATASH010000558 DATASH010000000
VERSION     DATASH010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5330
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683934"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VHV99_15300"
     CDS             <1..240
                     /locus_tag="VHV99_15300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636824.1"
                     /translation="LLNLDVHVSLQFRSQKQRNRPVPPLPYTCRSVPWTTTPVASFMH
                     LASTFANLFVAGGKPYLRASVGHQIKLWGFFEEVG"
     gene            complement(455..1558)
                     /locus_tag="VHV99_15305"
     CDS             complement(455..1558)
                     /locus_tag="VHV99_15305"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEX3636825.1"
                     /translation="MSEGSFVSPFAARFELRVPVVQAPMAGGATTPAMVAAVSNAGGL
                     GFLGAAALSPEKIASEVAAIRALTDRPFGVNLFVLEPAAPDEATVRHALEAIDPLRAE
                     FGLPPGQPLERYAPDFRAQLETLIELRVPVASFTFGLLAAEDVARLHANGLYVIGTAT
                     HVAEGVAWRDAGADAIAAQGAEAGAHRGTFIGAFEDALVGTMALVPQLVDATGLPVLA
                     AGGIMDWRGIVAAFALGAQAAVMGTAFLTCFESAIPQAWKTRVRSMSDTSTSVTRAIT
                     GRHARGIRNPLMQHLSESAHKVAPYPVQNALTQELRQTAGRANNGDYLSLWSGQGAPL
                     GRGRREGLGAAELVGQLDQEWHAVVARIAAIKG"
     gene            1832..2548
                     /locus_tag="VHV99_15310"
     CDS             1832..2548
                     /locus_tag="VHV99_15310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX3636826.1"
                     /translation="MSTTSRWIDIPAGNDSFGGNDSFGGYLALPKGGKGPAVIIIQEI
                     FGVNSHIRSVADQYAQDGYVALAPDVFWRVQPRVELTYDGADREKGIELMQKTNVDQA
                     VADIGAAAAALRAMPEVTGKVAAIGFCFGGRLAYLTAAQGTLDGAVAYYGGGIQNQLD
                     EAAKIKVPMQFHYGELDAHIPLSAVGQIQERFAGRTDAQFNIYPNADHGFNCSDRASY
                     NQHASALAHGRTLTFLGERL"
     gene            3016..4536
                     /locus_tag="VHV99_15315"
     CDS             3016..4536
                     /locus_tag="VHV99_15315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX3636827.1"
                     /translation="MQSDYLAHDAIGLAELVRSREASARELLDIAIARTEAVNPAINA
                     IVLKDYDAARQRASLDDANRANGANHTDGGSAQAALAGVPYLIKDLGAAVAGLRMSMG
                     SRHYRHFIPTQDAPVVALAKAAGLNIFAKTSTSELGQMPYTEPELFGACRNPWNLDHT
                     PGGSSGGAAAAVAAGIVPLAHAADGGGSIRIPASCCGLFGLKPSRGRVPRATPTAAVG
                     ELGIDHAVSRSVRDSALLLDLLGGNADRPLGAPGTFLGASREPCKPLNIAYVTDSMLA
                     PSLSADSRAALEDAAQLASSLGHNIEPVSLGIDFAAVRHAFLMLWSVTAEDMVLNADR
                     ITGRKPLRDEFEISTWVMAHVGRKLGERGLPGALEEQRRITERLTDLLSRYDVLLCAT
                     LAGATIKIGEMQPTAAERMQMRAVSAMPLEPLLKKLLTEASNKAFAWAGCTELFNLSG
                     QPAMSVPLYWNARGLPVGVQFAARNGGEAMLLRLAAQLEAARPWFDKRPPLMQARS"
     gene            complement(4604..4915)
                     /locus_tag="VHV99_15320"
     CDS             complement(4604..4915)
                     /locus_tag="VHV99_15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-potential iron-sulfur protein"
                     /protein_id="HEX3636828.1"
                     /translation="MKSSRRTFLITSIGVASTFALSRQAFADAPKVAETDPTAQALGY
                     KADASKVDKAKFAKYAAGQDCGNCSFYQGKPTDPYAGCPMFAGKQVASKGWCSAYNKK
                     A"
     gene            complement(5179..>5330)
                     /locus_tag="VHV99_15325"
     CDS             complement(5179..>5330)
                     /locus_tag="VHV99_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3636829.1"
                     /translation="PGEPAADFVIQWPAAHGVRGLVNLFGIESPGLTASLAIAQRVCE
                     LSGRA"
BASE COUNT          987 a         1706 c         1651 g          986 t
ORIGIN      
        1 cttttgaatc ttgatgttca tgtaagtctc cagtttcggt cccaaaaaca acgtaatcgc
       61 cctgtgcccc cgcttcccta cacgtgtcgc tcagtcccgt ggacgaccac gccggttgca
      121 tcgttcatgc acttggccag cacgttcgcc aacctgtttg tggcaggcgg caaaccgtac
      181 ttgcgcgcaa gtgtaggcca tcaaattaaa ttgtggggct tttttgagga agtgggatga
      241 acactaatag cgtttatcca acagcggccg attcgggctg gatagagagg ctggaaaacc
      301 ccatcccatg cgggtttccg ccaggtaggc gtttcatctg acagcaccgg cctaaagctt
      361 ttagcgtgtt aaaccgttaa tggttcgaat agactttgga tttaaaaaca ggcgcgtccc
      421 ggaccgttgc gaggcgcgcc tgagccgcgc tgaatcaacc tttgatcgcg gcgatccgtg
      481 caaccacagc atgccattcc tgatcgagtt gcccgaccag ttccgccgcg ccgaggcctt
      541 cgcgacgccc gcggccaagc ggtgcgccct ggccggacca gagagacaga tagtcgccgt
      601 tattcgcgcg gcccgcggtt tgccgcagtt cctgagtcaa cgcgttttgc accggataag
      661 gcgcgacctt gtgcgccgac tcgctcaaat gctgcatcaa cggattgcgt atgccgcgcg
      721 catgacggcc ggtgatcgcg cgtgttacgg atgtcgatgt gtcggacatc gaacgtacgc
      781 gggttttcca tgcctgcggt atcgcactct cgaaacaggt gagaaacgcg gtgcccatca
      841 cggcggcttg cgcgccgagc gcgaacgcgg cgacgatgcc gcgccagtcc atgatgccgc
      901 cggcggccag caccggcaga ccggtcgcat cgacgagttg cggcacgagc gccatggtgc
      961 cgaccaacgc atcctcgaat gcgccaatga aggtgccgcg atgcgcgccc gcctccgcgc
     1021 cttgcgcggc gattgcgtcg gcgcccgcat cgcgccacgc gacaccctcg gcgacatgcg
     1081 tcgcggtgcc tattacatac aggccgttcg catgcagacg ggcgacgtct tcggcagcga
     1141 gcagtccgaa cgtgaagctc gccaccggca cgcgcaattc gatcagcgtt tccagttgcg
     1201 cgcgaaaatc cggcgcatag cgttcgagcg gctgcccggg cggcaaacca aactcggcgc
     1261 gcaatggatc gattgcttcg agcgcgtggc gcacggtggc ttcgtccggc gcggccggct
     1321 cgagtacaaa caggttcacg ccgaaggggc gatccgtgag cgcgcgaatc gccgcgactt
     1381 cactcgcgat cttctcgggc gatagcgcgg cggcgcccag aaaaccgaga ccgccggcgt
     1441 tcgacaccgc ggcgaccatc gccggcgtcg tcgcgccgcc ggccatcggc gcctgcacaa
     1501 ccggtacccg caactcgaag cgcgccgcga acggactcac aaaactgcct tcactcatga
     1561 tcgattctcc ttccgacaat gcgctcgcgg catcggcgag ccagtactct ggactgtatc
     1621 gagcctggcg cgcgcggaga aatgaattat cgagaatgtt tcaatcgcct tacgagatcg
     1681 ttgctacgcg acgagcttcg tcaggcctcg cggggccccg ctgcaagccg tttcttttgg
     1741 tggcacactg accgcccgct tcagcattcg gccctctggc aatcattcag accgaccccg
     1801 cgtatcaact cactcgtaaa tggagagcaa catgagcacg acttcccgct ggatcgacat
     1861 tcccgccggc aacgacagct tcggcggcaa cgacagcttc ggcggctatc tggcgctgcc
     1921 caaaggcggc aagggaccgg ccgtcatcat cattcaggaa atcttcggcg tgaacagcca
     1981 tatccgttcg gtcgcggatc agtacgcgca agacggctac gttgcgctcg caccggacgt
     2041 gttctggcgc gttcagccgc gcgtcgaatt gacgtatgac ggcgcggacc gcgaaaaggg
     2101 catcgagttg atgcagaaaa ccaatgtcga tcaggcggtg gccgatatcg gcgctgccgc
     2161 cgcggcgttg cgtgcaatgc cggaagtgac aggcaaggtt gcggcgatcg gcttctgctt
     2221 cggcggccga ctcgcctatc tgactgccgc gcaaggcacg ctcgacggcg cggttgccta
     2281 ttacggcggc ggcattcaga accagctcga tgaagccgcg aagatcaaag tgccgatgca
     2341 attccactac ggcgaactcg acgcgcacat tccgctgtcg gctgtcggcc agattcagga
     2401 acgcttcgcc ggccgcaccg atgcgcaatt caacatctat ccgaacgcag atcacggctt
     2461 caactgctcg gaccgcgcgt cgtataacca gcacgcctcg gcgctcgcgc atgggcgcac
     2521 gctgaccttc ctcggtgaac ggctgtaatt cgcggttgtg attcgcggtt gtgattcggt
     2581 gcggttgtga tgcgcggccg tgatgcgcgg ttgtgatgcg cggttgtgat gcgcggctgc
     2641 gatacgcggc cgtgatacgc ggctgtggtg cgcggctgtg gtgcgcggct gcgatgcacg
     2701 gctgtgatac gcgcttataa acaacgcttg tcactcgcgc cgataactcg cagctttagt
     2761 tggcgcctgt aactcgcgtc catggctcgc aacttcaccc gatgcctgcc gctcgcgcct
     2821 acagctcaca ccttcaatcg gcacctgtaa ctcgcacgag tactgggtta ctctctcaaa
     2881 gacggcgcgc catcggcgcg tcgccttctt ccatgcttat cgatgatccg gggacgacgg
     2941 gacgtccggc aaagccattc atcattcgca ttggcaaccg gcaaatccaa cactcgatcg
     3001 agagggctcg ccgtcgtgca atcagactat ctcgcccatg acgccatcgg cctcgccgaa
     3061 ctggtccgaa gccgcgaagc gagcgcgcgc gagttgctgg atatcgcgat cgcccgcacc
     3121 gaggccgtca atcccgccat caacgcgatc gtcctgaagg actacgacgc agcgcgtcag
     3181 cgtgcctcgc tcgacgatgc gaacagagcg aatggcgcga accatacgga cggtggaagc
     3241 gcgcaagctg cactcgcggg cgtgccctat ctcatcaagg atctgggcgc agcggttgcc
     3301 gggctgcgca tgtcgatggg cagccgccac tatcggcatt tcattcctac ccaggacgca
     3361 ccggtcgtcg cactcgcgaa agcggcgggc ctcaacatct tcgccaagac cagtacctcc
     3421 gaactcggtc agatgcctta tacggagccc gaactgttcg gcgcgtgccg taacccgtgg
     3481 aatctcgacc acacgcctgg cggttcaagc ggcggtgcag cggctgcggt cgccgctggc
     3541 atcgtgccgc tcgcgcatgc agcggatggc ggcggctcga tccgcattcc cgcttcatgt
     3601 tgcgggttgt ttggcctcaa gccttcccgc ggacgtgtgc cgcgcgcgac gccgaccgcc
     3661 gcggtcggtg agctcggcat cgatcacgcg gtatcgcgca gcgtgcgcga cagcgcgttg
     3721 ctgctcgatc tgctgggtgg caacgcggac aggccgcttg gcgcgccggg gaccttcctc
     3781 ggcgcaagcc gcgagccatg caagccgctg aacattgcct acgtcaccga ttcgatgctg
     3841 gcgccctcgc tctccgccga ctcgcgcgct gcgctcgaag acgccgcgca actcgccagt
     3901 tcgctaggcc ataacatcga accggtgtcg ctcggcatcg atttcgcggc agtccgccat
     3961 gcgttcctga tgctgtggtc ggtgacggcg gaagacatgg tgctgaacgc cgatcgcatc
     4021 accggccgca aaccgctgcg cgacgaattc gaaatctcca catgggtgat ggcgcatgtc
     4081 ggccgcaaac tcggcgagcg tggcttgcca ggcgcgctcg aagaacagcg acgcatcacc
     4141 gaacgcctca ccgatttgct gagccgctac gacgtgctgc tgtgcgcgac gctcgccggc
     4201 gccacgatca agatcggcga aatgcagccg acggccgccg agcgcatgca gatgcgcgcc
     4261 gtcagcgcca tgccgctcga accgttgctg aagaagctgc tgaccgaagc gtcgaacaag
     4321 gcgttcgcgt gggcgggatg cacggagttg ttcaatctga gcggacaacc tgcgatgtcg
     4381 gtgccgcttt actggaatgc gcgcgggctg cctgtcggcg tgcagttcgc cgcgcgtaac
     4441 ggcggtgaag ccatgctgtt gcgactggcc gcgcagcttg aagcggcgcg cccgtggttc
     4501 gacaaacgac caccgttgat gcaggcgcgc agctaaagga aaggccgtcg cgacgttgcg
     4561 gcgatttgcc gcgtcactga gcgtgcgtgc ggcccaaacg taatcaggct ttcttgttgt
     4621 atgcgctgca ccaacccttg ctcgcgacct gcttgcccgc gaacatcggg cagcctgcat
     4681 aggggtcggt cggcttgcct tggtagaagc tgcagttgcc gcagtcctgg cctgctgcgt
     4741 acttcgcgaa cttggccttg tcgactttgc tcgcgtcggc cttgtagccc agtgcttgtg
     4801 cggtcggatc ggtctccgcg actttcggtg cgtcggcgaa agcctggcgc gacagcgcga
     4861 aggtggatgc cacaccaatg ctggtgatca aaaacgtacg acgggaagac ttcatgggga
     4921 cactccattt atattgaact gatcgccgtt ctagtgacag cggtccccaa ggatagcaat
     4981 gaagccgtcg cgcgtgacgg cccaaatgat agagataacc gaatttgtac taccccacgc
     5041 tcgaaagtcg ggcacggaag gcggggttgt tcacatgaaa cgcgaacgcg acatgagagt
     5101 ccgggggtat ggctgctaag tgcgcccgtt aagtgcgccc ccaggtgtgc ccgttaaggg
     5161 tgcccccaag tacgcccgtt aagcgcgtcc gctcagttcg cagacgcgct gcgcgatcgc
     5221 gagcgacgcc gtcaatcccg gcgactcgat accgaacaga ttgacgagcc cgcgcacgcc
     5281 atgcgcggcg ggccactgaa tcacgaagtc ggcggcgggt tcgccaggac
//
LOCUS       DATASH010000559         4331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_684154,
            whole genome shotgun sequence.
ACCESSION   DATASH010000559 DATASH010000000
VERSION     DATASH010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4331
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684154"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..1037
                     /locus_tag="VHV99_15330"
     CDS             165..1037
                     /locus_tag="VHV99_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3636830.1"
                     /translation="MEHVDWDDLRILVAIARGGTMRAAAAACDLSAPTLSRRLTELER
                     RLGEPLVDRVPSGCTVTAFGARVLAWAEQMEDLAHQIERAADVTGGAGAHGTVRINAV
                     EWPSYILLKLLGSFQDRLPGLAIEVLTSRRPYSLARREADIALWSECPDEGDLYVRRI
                     GRIRFGLFGSRDYYLRHKAAITRKEWDKLSFVGYDDRQPDHPATQWLKTLPGAPAPSL
                     RSSYGMGVFDGVLGGSGLGVLAQLAGDTTSDLVCIEKHIKALDQDVWMVLHPALRDSE
                     RIRTVANLIAEIFR"
     gene            complement(1124..1534)
                     /locus_tag="VHV99_15335"
     CDS             complement(1124..1534)
                     /locus_tag="VHV99_15335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636831.1"
                     /translation="MRFGHGLDDREADTHSAGTCPDGPARLREKFEHVWQQGGGDAEP
                     IVVDRYDKFGSLGVHIQLDAAARRRVFLRINAEFPCVSLRTPRPGPTNLSRESGLEIA
                     GAYGASTTLARIVKGVCTVAFTVTSGFVGGRRGA"
     gene            1895..2614
                     /locus_tag="VHV99_15340"
     CDS             1895..2614
                     /locus_tag="VHV99_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX3636832.1"
                     /translation="MLTTHQPKDNHLLSVLPKTEWERIAPHLVELDMPLGEVVYESGD
                     RLDYVYFPTTAIVSLLYVMEDGASAEIAIVGNEGLIGIALFMGGETTPSRAVVQSAGQ
                     AYRLGAHILKEEFHRAGPVQRLLLRYTQALITQMAQTAVCNRHHSIDQQLCRWLLLSV
                     DRLPSNELRMTQELIANMLGVRRSGVTEAAMKLQDAGLIRYSYGHIEVIDRPGLEARV
                     CECYGVVRREFDRLLPELKAV"
     gene            2680..3243
                     /locus_tag="VHV99_15345"
     CDS             2680..3243
                     /locus_tag="VHV99_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX3636833.1"
                     /translation="MYTRILVAIDGSETSGHAFDAALQLAHESGAQLQPLYVIDNPLM
                     AYDASGYDPTILREAFVEEGHRLLADALARMKHENVAGTPRMVDVAPIGEDISERIRT
                     TANEFNADLLVLGTHGRRGFRRLFLGSVAERVLRSSSCPVLLVPSRQAQTTQVESAKP
                     GHADVAAAMYEMPPRERGTTNPVETSD"
     gene            complement(3318..3917)
                     /locus_tag="VHV99_15350"
     CDS             complement(3318..3917)
                     /locus_tag="VHV99_15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HEX3636834.1"
                     /translation="MASVPIRTTLANPAPLGLAGFALTTWLLSMINAGWFSGESMGLV
                     LACALAYGGTAQAIAGMMELPRGNTFGATAFLSYGAFWWSFALFVLFLHDKVPPAFVG
                     WYLFLWGVFTFYMWLATFRSPRVLQFIFLALWITFGLLAGGEWTGSGLVRMAGGYMGL
                     ITATLAFYLSAADVINEVHGRVILPVGDPRIFSRHAVAL"
     gene            complement(3989..>4331)
                     /locus_tag="VHV99_15355"
     CDS             complement(3989..>4331)
                     /locus_tag="VHV99_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase"
                     /protein_id="HEX3636835.1"
                     /translation="IESALVANPLVAEAAVVGRADETTGEVVVAFVVLKGERPTGDQA
                     IRMATELRNWVGKEIGPIAKPKEIRFGENLPKTRSGKIMRRLLRSLAAGEEITQDVST
                     LENPAIFDQLG"
BASE COUNT          838 a         1370 c         1368 g          755 t
ORIGIN      
        1 gatagccgtt gagtcgtgtg atgggcaggt tcgccacact tgccgcccgc cgctgaaacc
       61 ttgaggcttc tttatcagat gctgggacga gtatggccag atccaatgct atcctcgcga
      121 tccacgttat gctatgccta atttcaaaat cgggcgtttc aaaaatggaa cacgtggact
      181 gggacgactt gcggattctg gtagcgatcg cgcgaggcgg aaccatgcga gcggctgccg
      241 cggcctgcga tctgagcgcg ccgaccctgt cgcgccgtct caccgagctt gaacggcggc
      301 tgggcgaacc gctcgtcgac cgggtgccgt caggctgtac ggtcacggca ttcggcgcgc
      361 gcgtgctggc gtgggccgag cagatggagg acctggccca ccagatcgag cgcgccgcgg
      421 acgtgaccgg cggcgcgggg gcgcacggca cggtgcggat caacgccgtc gaatggccat
      481 cttacatttt gcttaaattg ctcggctcgt tccaggaccg gctgcccggc ctcgccatcg
      541 aagtgctgac ctcgcgccgg ccctatagcc tggcgcgccg tgaagcggac atcgcgctgt
      601 ggtccgaatg ccccgacgag ggcgatctgt acgtgcggcg aatcggcagg atccgcttcg
      661 ggctgttcgg ttcgcgcgac tactatctgc gccacaaagc cgcgatcacg cgcaaggaat
      721 gggacaagct gtcgttcgtc ggttatgacg accggcaacc ggatcacccg gccacgcaat
      781 ggctgaaaac cctgcccggc gcgcccgcgc cttcgcttcg aagcagctat ggcatgggcg
      841 tattcgacgg cgtgctgggc ggctccgggc tcggcgtgct cgcccagctc gcgggcgaca
      901 cgacgagcga cctcgtctgc atcgagaagc acatcaaggc gctcgaccag gacgtgtgga
      961 tggtgctgca ccccgccttg cgcgatagcg agcggatccg gacggtggcc aatctcatcg
     1021 ccgagatctt tcgctaggcc ggggttcgaa gtcgtggggg cgtgggggcg tgggggcgtg
     1081 gagcgtaccc actgcgtagc cgaatagaag cacgcccggc tcgtcacgcg ccacgccggc
     1141 caccaacgaa cccagaagtc accgtgaacg ccaccgtgca gacacccttc acgatgcgtg
     1201 ccagagtggt gctggccccg tacgcaccag cgatttccaa accgctttcg cgcgacaggt
     1261 ttgtcggtcc cggacgcggc gtgcgcaacg aaacacaggg aaattccgcg ttaatgcgga
     1321 gaaatacacg ccgccgggcg gctgcatcga gctggatgtg cacgccgagg gagccgaact
     1381 tgtcatatcg gtcaacgaca atgggctcgg catctccgcc gccttgctgc cacacgtgtt
     1441 cgaacttttc tcgcagtcga gccggaccat cggggcaagt gccggcggaa tgggtatcgg
     1501 cctcccggtc gtcaaggccg tggccgaagc gcacggagga tccgcgacgg caaccagcga
     1561 cgggcccggt tgcggcagtg cgttcgtgct gagaatgcct gttgtcgtcg cgcagacccg
     1621 agcacgaggc gcgctccccg atttcagatg gcgttgacga tggcgcggaa tccccgcgta
     1681 gccgcgcagc acgccagcgt gaatctcacg gtggcgcgaa ctatacttta agcacgctac
     1741 gggcgccttc ggcattgacg ggcgcttgct tgccgtaccg tccagccggt cgatttcaaa
     1801 acggcaccgc gcggccggca agcaaactct tcagatcaat tttccgaatc cgaagcacgc
     1861 agccgttttg cagcaggaca ttgggaggcg gttcatgttg accacacatc aaccgaaaga
     1921 taatcatctg ctttccgtac tgccgaaaac ggaatgggag cgcattgcac cgcacctcgt
     1981 cgagctcgat atgccgctcg gcgaggtcgt gtacgaatcg ggcgaccggc tggactatgt
     2041 ctacttcccc accaccgcga ttgtctcgct gctctacgtg atggaagacg gcgcctccgc
     2101 cgagatcgcg attgtcggca acgaagggct gatcggcatc gcgctcttca tgggcggcga
     2161 aacgacaccg agccgcgcgg tcgtgcaaag cgccggacag gcgtaccggc tcggcgcaca
     2221 cattctgaaa gaagagtttc atcgtgccgg acccgtacag cggctactgc tgcgttacac
     2281 gcaggcactc atcacgcaga tggcgcaaac ggccgtgtgc aaccggcatc attcgatcga
     2341 ccagcagctg tgccgctggc tgctgttgag cgtcgaccgt ttgccgtcca acgaattgcg
     2401 catgacccag gagttgattg ccaacatgct gggcgtgcgc cgttcgggcg tcaccgaggc
     2461 ggccatgaag ctgcaggatg cggggttgat ccgctacagc tatggccata tcgaagtgat
     2521 cgaccgcccg ggtctcgaag cacgggtgtg cgaatgttac ggggtggtga ggcgggaatt
     2581 cgaccgtctg ttgcccgagc tgaaagccgt ctagacgctg cgcacagcat ccggctcagc
     2641 cacttttctg acgccgccat tgaactcacc gaggtctaca tgtacacacg cattctggta
     2701 gcgatcgacg gcagcgaaac ctccgggcac gcgttcgacg ccgctttgca gctcgcgcac
     2761 gagagcggcg cgcaattgca acccctctat gtcatcgaca atccgctgat ggcgtatgac
     2821 gcatccggct acgatccgac gatcctgcgt gaagcattcg tggaagaggg ccatcggttg
     2881 ctggcggacg cactggcgcg tatgaagcac gaaaacgtgg ccggcacgcc gcggatggtg
     2941 gacgtcgcgc cgatcggcga ggacatttca gagcgtatcc gcacgacggc gaacgaattc
     3001 aatgcggatc tgctggtgct cggcacgcac ggcaggcgtg gcttcaggcg tctgtttctc
     3061 ggcagcgtcg ccgagcgcgt gctgcgcagt tcgagctgtc cggtgttact ggtgccgagc
     3121 cggcaggcgc agaccacaca agtggagtcc gccaaaccgg ggcacgccga tgtcgcggct
     3181 gcaatgtacg agatgccgcc gcgtgagcgt ggtaccacca atcctgtcga gacgagcgac
     3241 taggccgcga tgatcacctt cagcgggtgt gcgtcggcgg acatgcgcaa tccgcagccg
     3301 ccgcccgttc tggacagtca gagcgccacg gcatgccggc tgaatatccg cggatcgcca
     3361 accggcagaa tcacgcggcc atgcacctcg ttgatcacgt cggctgcgga gaggtagaac
     3421 gcgagcgtcg ccgtgatcag ccccatatag ccgcccgcca ttcgtaccag acctgaaccc
     3481 gtccactctc ctccagccag caggccgaag gtgatccaga gcgccagaaa aatgaactgc
     3541 agcacgcgag gcgagcgaaa ggtcgccagc cacatataaa acgtgaagac gccccaaagg
     3601 aacagatacc agccgacgaa cgcgggtggc accttgtcgt gtaaaaagag cacgaacagt
     3661 gcaaacgacc accagaacgc gccataactc agaaaggccg tcgcgccgaa ggtattaccg
     3721 cgcggcaact ccatcatgcc ggcaatggcc tgcgcagtcc cgccataagc gagcgcgcag
     3781 gccagcacca ggcccatgga ttcgcccgaa aaccagccgg cgttgatcat gctcagcagc
     3841 cacgtcgtca atgcaaaacc ggcgagaccc agtggcgcgg ggttcgctaa tgtggttcgg
     3901 atcggtacgc tggccatagc ctgctcctga agattgaaaa ctttccggcg gtccggcggt
     3961 ccggcgctct cgcggcattc gcgagtcatc acccgagctg atcgaaaatc gccggatttt
     4021 ccagcgtgga gacgtcttgc gtgatctctt cgccggctgc aagcgagcgc aacaagcggc
     4081 gcatgatctt gcctgagcgg gttttcggca gattctcgcc gaagcggatt tccttgggct
     4141 tcgcgatcgg cccgatttcc ttgccgaccc agttgcgcaa ttccgtcgcc atcctgatcg
     4201 cctggtcgcc ggttggccgc tcgcctttca acacgacgaa cgcgaccacg acttcgccgg
     4261 tcgtctcgtc ggcacggccg accaccgccg cctcagcgac gagtggattc gcgacgagtg
     4321 ccgactcgat c
//
LOCUS       DATASH010000560         5737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_91545,
            whole genome shotgun sequence.
ACCESSION   DATASH010000560 DATASH010000000
VERSION     DATASH010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5737
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91545"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..1177
                     /locus_tag="VHV99_15360"
     CDS             185..1177
                     /locus_tag="VHV99_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018432550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3636836.1"
                     /translation="MTIRNLAVNFSGLPAVDRINLDVAAGEVVGVVGESGSGKSVTMM
                     ALMGLIDAPGKVTADEITFNGKNLLKASARERRKIIGKDIAMVFQDALTSLNPSYTVG
                     YQIKEVLKLHEGLRGSALDKRALELLDQVGIPDAKSRITSFPHQMSGGMNQRVMIAMA
                     IACNPKLLIADEPTTALDVTIQAQIMELLIKLQKERGMALVLISHDLAVVSEVAQRVA
                     VMYAGEVIETNRVPDIFAAPHHPYTEALLAAIPEHNVGAVRLAALPGMVPGRDDRPKG
                     CLFAPRCKYVVDDCLKARPALAPMQGHAEVARVRCIKPLNLSGDANVHTHGGAR"
     gene            1174..2193
                     /locus_tag="VHV99_15365"
     CDS             1174..2193
                     /locus_tag="VHV99_15365"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004523051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein"
                     /protein_id="HEX3636837.1"
                     /translation="MNAVPEMRRQSDHAGDHVLVADKLARYYTVKRGMFSQGTVKALN
                     GVSFALERGKTLAVVGESGCGKSTLARQLTMIEAPSAGRLLIDGEDVAGADHAKIAAL
                     RRRVQMVFQNPFASLNPRKTVEQTLGEPLAINTQLSTTERAERIAQMMRTVGLRPEHA
                     KRYPHMFSGGQRQRVAIARAMILDPQIVVADEPVSALDVSIQAQILNLFMDLQEQFKT
                     SYVFISHNLSVVEHIADDVMVMYFGGVAELGDKKRIFSKPRHPYTRALMSATPSIFEA
                     DRTIKIKLQGEMPSPLNPPSGCTFHQRCPYVIDRCRSEEPKLREVDGRQVSCHRAEEV
                     GDVDA"
     gene            2186..3337
                     /locus_tag="VHV99_15370"
     CDS             2186..3337
                     /locus_tag="VHV99_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HEX3636838.1"
                     /translation="MPDGVAARRLTRRSRDGVRNAAFARRWGARALTGAALFGVCSVF
                     HLSAGPVGVAHAAGAAANTPAQAGRPAMPVPNLPAPPRATLPGFNPPPAAPGTTASGP
                     VQTQPARMPFYVATRGTTTIYVLGTLHVGDPADYPPRQPFRAPILGALAASPTLALEL
                     SPDELLVSQDDVSKYGVCRRDCLPGLLPEPLWRKLAFRLRGNPAALDEIKKMRPWLAS
                     LVVETHDSLSAGLQTEYGTEAQLQNVYIRTRGKIVGLETLPQQMRAFTGLTLAQQREM
                     LAQDLVQTPAENVDDVKTLRRLWRVGDADAIAAWQAAKSEKLARDKRVSDSIDNRIMY
                     ERNRRFVSRMLLIAGPNKPVFVAIGALHLGGRKGVLQLLRQHGFVVDAG"
     gene            3510..3815
                     /locus_tag="VHV99_15375"
     CDS             3510..3815
                     /locus_tag="VHV99_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEX3636839.1"
                     /translation="MYRRQYRWYVRFEWDEIKNQINIRKHGIDFRDAIDVFDHPVLTA
                     IDEREDYGEERWIALGWMAAIVGVVVYVERDADVIRIISARKATKHEVKRYKHSVWH"
     gene            3857..4117
                     /locus_tag="VHV99_15380"
     CDS             3857..4117
                     /locus_tag="VHV99_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEX3636840.1"
                     /translation="MMSKSTGTDWKRLAKTDDAQIDTSDVPELGDDFFSRAELHVPPK
                     QAVTMRLDADVLSWFKEQGAGYQTRINKLLRAYMLAQQRRRP"
     gene            complement(4204..>5737)
                     /locus_tag="VHV99_15385"
     CDS             complement(4204..>5737)
                     /locus_tag="VHV99_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing
                     glycosyltransferase family protein"
                     /protein_id="HEX3636841.1"
                     /translation="SNRGLVAAALGHLEEAMICYDQALQLQPDFADARNNFGVALQAQ
                     GALEEAIGQYQLALAVNPSLVDAHLNLGTALSKLGRYDEALACYQTVSALDPQSAEAH
                     FNAGNAYNAQGDEAAAIASFERALALRGNYAEAHINLGSLIGKLGDYAGAESQYRRAV
                     ALKPNPTNLVCLGGSLGAQGRLDEEEGFYRHALALDPDYADAHQNLAWLLLKRGDYKH
                     GWAEFAKRWRKSDYEAIAVPGVVEWHGEPLDGCRLLLVGEQGFGDHFQFLRYARVLEQ
                     LGATVDICVREPLLPLVERIPGVHRAFSGKPDGQYDFWVPMMNVPSCVGTELPTIPAD
                     VPYVFADKAKIKTWRKRVDAAGKSKRKVGLVWSGSPTFGNDRYRSMTLADLNMLSELK
                     NVAWYALQKGPAHDQLTRAPEAFRAHDFTADLHNFDDTAALVMNLDLVIAVDTGVAHL
                     AAALGKPVWVLLPANSDWRWLEARNDSPWYPAMKLFRQTMLGDWTPVIKRVSEELRDS
                     SL"
BASE COUNT         1095 a         1768 c         1825 g         1049 t
ORIGIN      
        1 ctctccatcc tgatctcggt gcttgcgatc aatctgctcg gcgacgggct gcgcgacgca
       61 ctcgatccca aactgaaacg gatggcctga catggaacaa tccccacgtt cacatcagtc
      121 gctgccccct gatgcggtgg gtcagcacgc ttcaggcggc cgtgcgcgta ctgacggcac
      181 cttattgacc atccgcaatc tcgcggtgaa cttcagcgga ctgcccgccg tcgaccgcat
      241 caacctcgac gtcgcggcgg gcgaagtggt cggcgtggtc ggcgaatcgg gctcgggtaa
      301 gagcgtcacg atgatggcgc tgatgggcct gatcgacgca cccggcaaag tcaccgcgga
      361 tgagatcacc ttcaatggca agaatttgct gaaggcctcg gcgagagagc gccgcaagat
      421 catcggcaaa gacatcgcga tggtgttcca ggacgcgctc accagtctga atccgagcta
      481 cacggtcggc tatcagatca aggaagtgct gaagctgcac gaaggcctgc gcggcagcgc
      541 gttggacaaa cgcgcactgg aactgctcga ccaggtcggc attcctgatg cgaagagccg
      601 catcacgtca tttccacatc agatgtcggg cggcatgaac cagcgcgtga tgatcgcgat
      661 ggcgatcgcc tgcaacccga agctgctgat cgccgacgaa ccgaccaccg cgctcgacgt
      721 gaccatccag gcgcagatca tggagttgct gatcaaactg cagaaggagc gcggcatggc
      781 gctcgtgctg atctctcacg atctggcggt ggtgtccgag gtcgcgcagc gcgtcgcggt
      841 catgtacgct ggcgaggtga ttgaaaccaa ccgcgtgccc gacatcttcg ccgcgccgca
      901 tcatccgtat acggaagctt tgctggcggc gattcccgag cacaatgtcg gcgcggtgcg
      961 ccttgctgcg ctgccgggca tggtgccggg ccgcgacgac cggcccaaag ggtgtctgtt
     1021 cgcaccgcgc tgcaaatacg tggtcgacga ctgcctgaag gcgcggcccg cactggcgcc
     1081 gatgcaaggt cacgcggaag tcgcgcgcgt gcgctgtatc aaacccctga acctgagcgg
     1141 cgacgccaac gttcacaccc atggaggcgc acgatgaatg cagtacccga aatgcggcgc
     1201 cagtcggacc atgcgggcga tcacgtgctg gtggccgaca agcttgcgcg ttactacacg
     1261 gtcaagcgcg gcatgttcag ccagggcacg gtcaaggctt tgaacggcgt gtcgttcgcg
     1321 ctcgaacgcg gtaagacgct agcggtggtc ggtgaatccg gttgcggcaa gtcgacgctt
     1381 gcccgtcagc tcacgatgat cgaagcgccc agcgcgggcc gcctgttgat cgacggtgaa
     1441 gacgtggccg gtgccgatca cgcgaaaatc gccgcactgc ggcggcgcgt acagatggtg
     1501 tttcagaatc cgtttgcgtc gctgaatccg cgcaagactg tcgagcagac acttggcgag
     1561 ccgctcgcga tcaacacgca actgagcacc accgaacgcg ccgaacggat cgcgcagatg
     1621 atgcgcaccg tcggcctgcg tccggagcat gcaaagcgct atccgcatat gttttcgggc
     1681 ggtcagcggc agcgtgtggc gattgcccgg gcgatgatcc tcgatccgca gatcgtggtc
     1741 gccgatgaac cggtgtccgc gctcgacgtg tcgatccagg cgcagattct gaatctgttc
     1801 atggatctgc aggagcagtt caagaccagc tacgtgttca tctcgcacaa tctgtcggtg
     1861 gtcgagcata tcgccgacga cgtgatggtg atgtacttcg gcggcgtggc ggagctcggc
     1921 gacaagaagc ggatcttctc gaagccacgg catccctaca cgcgtgcgct gatgtcggcc
     1981 acgccttcca tcttcgaagc ggatcgcacg atcaagatca agctgcaggg tgaaatgccg
     2041 tcgccgctga atccgccctc gggctgtacg ttccatcagc gctgcccgta tgtgatcgac
     2101 cggtgccgca gcgaagaacc gaagttgcgt gaagtggatg gccgtcaggt gtcgtgtcac
     2161 cgcgccgagg aggtggggga cgtggatgcc tgatggagtg gcggcgcgtc gtcttacgcg
     2221 ccgctcgcgt gatggcgtcc gcaacgctgc tttcgcgcgg cgctggggag cgcgtgcact
     2281 gaccggtgct gcgctcttcg gcgtgtgttc tgtttttcac ctgagcgcgg ggccggtcgg
     2341 cgtcgcgcat gccgcggggg cggccgccaa tacgcccgct caggcggggc ggccggccat
     2401 gccggtgccc aatctgccgg cgccgccgcg cgccaccctg ccgggcttca atccgccgcc
     2461 cgccgcgccc ggcaccacgg caagcggccc ggtgcaaacg cagccggcgc gcatgccgtt
     2521 ctatgtcgcg acacgcggca ccacgaccat ctacgtgctc ggcacgttgc acgtcggcga
     2581 ccccgccgac tatccgccca ggcagccgtt ccgcgcgccg atcctcggtg cactggctgc
     2641 ctcgccgacg ctggcgctgg aactctcacc cgacgaactg ctggtctccc aggacgacgt
     2701 gtcgaagtac ggcgtatgcc gccgcgactg tctgccgggc ctgctgcccg agccgttgtg
     2761 gcgcaagctg gccttccgtc tgcggggcaa tcccgcggcg ctggatgaaa tcaagaagat
     2821 gcggccatgg ctcgcgtctc tggtggtcga aacccatgat tcgttgagtg ccggcttgca
     2881 aaccgagtac ggcacggaag cgcaattgca gaacgtctat atcaggacgc gcggcaagat
     2941 cgtcggtctc gaaaccttgc cgcagcagat gcgcgcgttc accggtctca cgctcgcgca
     3001 gcagcgcgaa atgctcgcgc aggacctggt gcagacgccc gcggaaaatg tcgacgatgt
     3061 gaagacgcta cgccgcctat ggcgcgtggg cgatgccgat gcgattgcgg cctggcaggc
     3121 cgcgaaatcc gaaaagctgg cgcgcgacaa gcgggtctcc gattcgatcg acaacagaat
     3181 catgtacgag cgcaaccggc gcttcgtctc gcggatgctg ttgatcgccg gaccgaacaa
     3241 gccggtgttc gtggcgatcg gtgcgttgca tctgggcggc cgcaagggcg tgctgcagct
     3301 tttgcggcag cacgggtttg tggtggatgc ggggtgagat tcgtctcagc cttgcgcgag
     3361 acttttgtgc ggctgaccac agtcttaacc ggggtaggcc gttcggccag aataggaacc
     3421 ggtatttgga cagctagtcc gacgctgacg tgcgccgcaa aatgctcgcg acgcacgcgg
     3481 ccatggctgc ccattgcatg ctatgatgta tgtacagacg tcaatacagg tggtatgtgc
     3541 gttttgagtg ggacgaaatc aagaatcaaa tcaacatccg taagcacggc atcgacttca
     3601 gagacgccat cgacgtgttc gatcatcccg tgctgacggc gatagacgaa cgggaagatt
     3661 acggtgagga acgctggatc gcgctaggct ggatggccgc gatcgtaggt gtggtggtgt
     3721 atgtcgaacg tgatgcggat gtgatccgca tcatttcggc ccgcaaagcg acgaagcacg
     3781 aggtcaaacg ctacaagcac agcgtctggc attgacgggg gaatcttggc gaatggtaga
     3841 aatgagcacg ggtgaaatga tgagcaaatc aacaggaacc gactggaaac gactcgcaaa
     3901 aacggacgac gcgcaaatcg acacgagcga cgtgccggaa ttgggcgacg actttttttc
     3961 ccgtgccgag ctgcacgttc cgccgaaaca ggctgtcacg atgcgcctgg atgcggatgt
     4021 gctgagctgg ttcaaggaac aaggcgctgg ctatcagaca cgcatcaaca agttgttgcg
     4081 cgcttacatg ctggcgcagc agcgtcggcg tccttaaggc acggcgccaa ggcattctgc
     4141 ctgcgtacga acggtcgcga aggcgctgcg cagaaacact cgaaatccgc gcagcgcagg
     4201 tttttataga ctgctgtcgc gtaattcttc cgacacgcgt ttgatcacgg gtgtccaatc
     4261 gcccaacatc gtttgccgga acaatttcat cgccggatac cacggcgaat cgttgcgcgc
     4321 ttcgagccag cgccagtccg agttggcggg cagcagcacc cacaccggct tgccgagcgc
     4381 ggcggccagg tgcgccacgc cggtgtccac ggcgatcacc aggtcgagat tcatgacgag
     4441 tgcggcggtg tcgtcgaagt tgtgcagatc ggcggtgaaa tcgtgggcac ggaaagcctc
     4501 gggcgcacgg gtcagctgat cgtgcgcggg gcctttctgc agcgcgtacc acgcgacatt
     4561 tttcagctcg cttagcatgt tcagatcggc caatgtcatc gagcgataac gatcattgcc
     4621 gaacgtcggg ctgccggacc acaccagacc cactttgcgt ttcgatttgc cggctgcgtc
     4681 tacccgctta cgccatgttt tgatctttgc cttgtcggcg aacacatatg gcacatccgc
     4741 gggaatagtc ggcagttcgg tgcccacgca cgacggcacg ttcatcatcg gcacccagaa
     4801 atcgtactgg ccgtcaggtt tgccgctgaa tgcgcggtgc acgcccggga tacgctcaac
     4861 cagcggcagc agcggctcac gcacgcagat atcgacggtc gcgccgagct gctcgagcac
     4921 ccgcgcataa cgcagaaact ggaagtggtc gccgaaaccc tgttcgccga ccaacaacag
     4981 gcggcatcca tcgagcggct cgccatgcca ctcgaccaca cccggcacgg cgatcgcctc
     5041 atagtcgctc ttgcgccagc gcttcgcaaa ctccgcccaa ccgtgcttgt aatcgccgcg
     5101 cttgagcaac agccacgcga ggttctgatg cgcatccgca taatctggat cgagcgccag
     5161 tgcgtggcga tagaagcctt cctcttcatc cagacggccc tgcgcgccga gcgatccgcc
     5221 gaggcaaacg agattcgttg ggttcggttt caatgcgacc gcacgccgat actgcgattc
     5281 ggcgccggcg tagtcaccga gcttgccgat caggctgccg agattgatgt gcgcctcggc
     5341 ataattgccg cgcagtgcca gggcccgttc gaaactcgca atggcggcgg cctcatcgcc
     5401 ttgtgcgttg tatgcattcc ccgcattgaa atgcgcttcc gccgactgtg gatcgagcgc
     5461 gctgacggtc tgatagcagg ccaacgcctc gtcgtagcgg ccgagcttgc tcagcgccgt
     5521 gccgaggttc agatgcgcgt cgaccagcga cggattcact gccaacgcca attgatactg
     5581 cccgatcgct tcctccagcg cgccttgtgc ttgcagtgcg acaccgaagt tgttgcgcgc
     5641 gtcggcgaaa tccggctgca actgcaacgc ctggtcatag cagatcatcg cttcttcgag
     5701 atggccgagg gcggcggcga cgagcccgcg gttgctc
//
LOCUS       DATASH010000561         1708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6620345,
            whole genome shotgun sequence.
ACCESSION   DATASH010000561 DATASH010000000
VERSION     DATASH010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6620345"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="VHV99_15390"
     CDS             complement(<1..497)
                     /locus_tag="VHV99_15390"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HEX3636842.1"
                     /translation="MRRRECITLLCGAVVWPIAARAQQPAMPVIGFLFGASPFAPYQA
                     AFRQGLKEAGYVEGQNVVIEYRWAAGQYERLPALVADLINRQVAVIAAPADPSALAAK
                     AATATIPIVFFSGSDPVKLGLVTDLNRPGGNVTGVSILSFTLLTKQLELICELVPTAA
                     TVAFLA"
     gene            671..>1708
                     /locus_tag="VHV99_15395"
     CDS             671..>1708
                     /locus_tag="VHV99_15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX3636843.1"
                     /translation="MPRQFDTAVVTIGIDPGKNTLHLVGLDARGEIVLREKVARAKIV
                     SRFANVPPCLIGIEAGMGTHYVTRGLLALGHDVRQVPPFYAKPFRQTHKNDFRDAHAV
                     AEAVQRPTTRCVPPKTDEQLDLQALHRVRYRLVGERTAIINQIRCFLLEHGLTVRQQL
                     HWLRHALPDILAQRTDVLSPRMVRILEDLAQDWHRLDDRIEAVSGEIEALAQTTESCR
                     HLMTIPGIGPIISSAVVAAIGNGIAFTRGRDFAAWLGLVPKQLSTGDRTILGRITKRG
                     NRYLRMLFMQAARVVLLRPRNWMKHGFGPWLAAAAQRLHHNVLATALANKLARIAWSV
                     LAQHRVYEARLS"
BASE COUNT          353 a          523 c          511 g          321 t
ORIGIN      
        1 gcgagaaaag cgactgtcgc ggctgtgggg actagctcgc aaatcagctc cagctgtttc
       61 gtcagtaacg tgaaagacaa gatgctcacg ccggtaacat tgccgcctgg ccgattgaga
      121 tcggtgacga ggccgagttt cacgggatcg ctgccgctga agaagactat cggaattgtc
      181 gcagtcgccg ccttagccgc gagtgccgag gggtcggcgg gcgcggctat cactgccacc
      241 tgccgattga tcaaatcggc caccagcgcc ggcaatcgtt cgtattgtcc cgcagcccag
      301 cgatattcga taaccacgtt ctggccttcg acatagccgg cttctttcaa gccctgacgg
      361 aacgcggctt ggtaaggcgc aaatggggac gcgccgaaga ggaacccgat caccggcatc
      421 gctggctgct gcgcgcgcgc cgcaattggc cacacgaccg cgccgcacag aagcgtgatg
      481 cactcgcgcc gccgcatatc gactcccgag tctcggggcc agcattgtat cctctgaaac
      541 aggcactttg acagggctga aaagcattgg cgcagtgcac agctaatgtc tcagaatggg
      601 atggaccggc cgtgctcccg ccaaacccgc gagcatactt gttgggggca atcaggagga
      661 ggatcacgcc atgccacgcc agtttgacac cgcagtcgtc accattggta tcgatcccgg
      721 caagaacaca ctgcacttgg ttggcctcga cgcccgagga gagatcgtgc tgcgtgagaa
      781 ggtagcacgc gccaaaatag tgtctcggtt tgcgaatgtg ccgccatgcc tgatcgggat
      841 agaggcaggc atgggaacgc actatgtgac ccgtgggctg ctcgccctcg gtcacgacgt
      901 gaggcaagtg ccgccgttct acgccaagcc tttccggcag actcacaaga atgactttcg
      961 agacgcgcat gcggtcgctg aagccgtgca acgtccgacg acacggtgcg tgccgccgaa
     1021 aacggacgag cagctcgatc tgcaggcatt gcatcgtgtg cgctaccgcc tggtgggcga
     1081 gcggaccgcg atcatcaatc agatacgctg ctttctcctg gagcacggcc tcacggtgag
     1141 gcaacagctc cactggttgc gccatgcatt gccagacatc cttgcccaac gcacagacgt
     1201 cctgtcaccg cggatggttc gcatcctaga agatctggct caagactggc accgcctcga
     1261 cgatcgcatc gaggccgtga gtggcgaaat cgaagcgctt gcccagacca cggagtcttg
     1321 tcggcatttg atgacaatac cgggcatagg tccgatcatc tcgagcgccg tcgtcgccgc
     1381 gatcggcaat ggaattgcct tcacacgcgg ccgcgatttt gcggcatggc tcggtttggt
     1441 ccccaagcag ttatcgacag gtgatcgaac gatcctcggg cgcatcacca aacgcggcaa
     1501 tcgctacctt cgcatgctct tcatgcaggc cgctcgcgtc gttctgctgc gaccaaggaa
     1561 ctggatgaag cacggctttg gaccttggct tgccgctgcg gctcaacgcc tacaccataa
     1621 tgtgctggcg acagctctgg ctaacaagct ggcccgcatc gcgtggagcg tgctggcaca
     1681 acatcgtgta tacgaggcgc gtctcagc
//
LOCUS       DATASH010000562         3575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_884812,
            whole genome shotgun sequence.
ACCESSION   DATASH010000562 DATASH010000000
VERSION     DATASH010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884812"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="VHV99_15400"
     CDS             complement(<1..829)
                     /locus_tag="VHV99_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008352546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HEX3636844.1"
                     /translation="MKLFLRFVFSALAFVAAVAALPQAAQAERLKDLVSFQGVRDNPL
                     IGYGLVVGLDGTGDQTTQTPFTTQSLVNMLGNLGITLNAATTQNMQLKNVAAVMVTAV
                     LPPFSRPGEAIDVTVSSLGNAKSLRGGTLLMAPLKGADGQVYAMAQGNLAVGGAGASA
                     NGSRVQVNQLAVGRIPGGALVERAVPAAVTQAGGVMTMELNTLDFDTTQKIVAAVNND
                     FGMGTAIALDGRTIQLRAPTDPSQVVSFMAQLENIDVRPAQAAAKVIVNARTGSIVMN
                     "
     gene            complement(832..1524)
                     /locus_tag="VHV99_15405"
     CDS             complement(832..1524)
                     /locus_tag="VHV99_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016346610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HEX3636845.1"
                     /translation="MSSQFSRFSRIRSTSVLALVALAACALVPKEPITQQPMTARPPA
                     APLNSASPGAIYNAGYAGRPLFEDQRPRNTGDIITIVIAENVNATKSSAANAARNASE
                     AFAVPVTPGIFGGLFHNTNLAASGSNGFNGAGGASAANTFNGTLTVTVTGVLPNGNME
                     VSGEKQMLINQGNEFVRFSGVVNPTTISGTNTVFSTQVADAKIEYSAKGYIDESQQMG
                     WLQRFFLNVAPF"
     gene            complement(1580..2368)
                     /gene="flgG"
                     /locus_tag="VHV99_15410"
     CDS             complement(1580..2368)
                     /gene="flgG"
                     /locus_tag="VHV99_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HEX3636846.1"
                     /translation="MNRSLYIAATGMNAQQSQMDVISNNLANVSTNGFKGSRAVFEDL
                     LYQTVRQPGANSTQQTELASGSQIGTGVQQVATERLYTQGNLSQTGNSKDVAINGNGF
                     FQVTMPDGTTAYTRDGSFQTNAQGQLVTSSGYQVTPAITIPTTATGITIGSDGTVTVT
                     QQGNAANTTVGQLQLATFVNPAGLDARGENLFAETASSGNANVVTPGLNGAGQLQQGY
                     VEASNVNVVQELVNMIQTQRAYEINSKAVTTSDQMLQTLSQMQV"
     gene            complement(2392..3147)
                     /gene="flgF"
                     /locus_tag="VHV99_15415"
     CDS             complement(2392..3147)
                     /gene="flgF"
                     /locus_tag="VHV99_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010112969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HEX3636847.1"
                     /translation="MDRLIYTAMSGAAQALEQQSVTANNLANASTTGFRAQLSAFRQV
                     PMNFENGASGDATTRTFVLSSTPSADYTPGPIQQTGNPLDVAVQGPGWLSVQTEDGGE
                     AYTRAGNLHVDANGQLVTANNLPVLGNAGPMAIPPGAEVTIGKDGTVTALMPGDPPTA
                     IATVDQLKLVNPDAGTLTRGDDGLFRTADNQPADADPTVTVAPASLEGSNVNAVSAMV
                     DMITNARQFQMQTKLLDSANSNDQSANKLLDFS"
     gene            complement(3183..>3575)
                     /locus_tag="VHV99_15420"
     CDS             complement(3183..>3575)
                     /locus_tag="VHV99_15420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HEX3636848.1"
                     /translation="TTGLVSSSQDGFSSSELAGFSVGTDGTLTGTYTNGKTQALGQIA
                     LATFANQNGLQDLGGNNYQQTAASGLAQVSVPGSTNHGSLQGGAVENSNVDLTSSLVD
                     LITAQRNYQANAQTIKTQQTVDQTLINL"
BASE COUNT          580 a         1108 c         1148 g          739 t
ORIGIN      
        1 ggttcatcac gatagaacca gtgcgcgcat tcacgatcac cttggccgcg gcctgcgccg
       61 gccggacatc gatattctca agctgcgcca tgaacgacac cacttgcgac ggatcggtcg
      121 gcgcgcgcaa ctggatcgtg cggccgtcga gcgcgattgc cgtgcccatg ccgaaatcgt
      181 tgttcactgc cgccacgatc ttctgcgtgg tgtcgaagtc gagcgtgttc agttccatcg
      241 tcatcacgcc gccggcttgc gtcaccgcag cgggcaccgc gcgttcgacc agcgcaccgc
      301 cgggaatgcg tccgaccgca agctggttga cctggaccct gctgccgttc gcgctggcgc
      361 ccgcaccgcc gactgcgaga ttgccctgcg ccatcgcgta gacctggcca tcggcgcctt
      421 tgagcggcgc catcaataac gtaccgccgc gcaggctctt cgcattgcca agcgatgaca
      481 cggtcacgtc gatcgcttcg cccggccgcg agaacggcgg caatacggcc gtcaccatca
      541 cggcggcgac gttcttcaac tgcatgttct gcgtagtcgc tgcattcagc gtaatgccga
      601 ggttgccgag catgttgacc aggctctgcg tggtgaacgg cgtctgggtg gtctggtcgc
      661 ccgtgccgtc gagcccgacc acgaggccat agccgatcaa cgggttatca cgcacgccct
      721 ggaacgaaac caggtccttg aggcgctccg cctgcgcggc ctgcggaagt gcggcaactg
      781 cggcaacaaa cgccagcgca ctgaaaacga agcgaagaaa gagtttcatc gtcagaaggg
      841 cgccacgttg aggaagaagc gctggagcca gcccatctgc tgcgattcat cgatataacc
      901 cttcgccgaa tattcgatct tcgcgtccgc cacctgggtg gaaaagaccg tgttggtacc
      961 ggagatggtc gtcggattga cgacgccgga gaagcgcacg aattcgttgc cctggttgat
     1021 gagcatctgt ttctcgccgc tcacttccat gttgccgttg ggcaggacgc cggtgacggt
     1081 gaccgtcagc gtgccgttga aggtattggc tgcgctcgcg ccgcctgcgc cgttgaagcc
     1141 gtttgagccg ctggcagcca ggttggtgtt gtggaacagg ccgccgaaaa tacccggcgt
     1201 gaccggcacg gcgaaggcct cgctggcgtt acgcgccgca ttcgctgccg acgacttcgt
     1261 cgcattgacg ttttctgcaa tcacgatggt gatgatgtcg ccggtattgc gcggccgctg
     1321 gtcttcaaac agcggccgtc ccgcgtagcc cgcgttgtag atcgcgccgg gcgatgcgga
     1381 attgagcggc gccgccggcg ggcgcgccgt catcggctgc tgggtgatcg gctctttggg
     1441 cacgagcgcg caggccgcca aggcgaccag cgccagcacc gatgtgctgc ggatgcgtga
     1501 aaagcgtgaa aattgtgatg acatcttcgt ggtccttcgg caatgacgct gctgatgcag
     1561 ttgatgcggt tggtgcaact taaacctgca tctggctcag cgtctgcagc atctggtccg
     1621 acgtggtcac ggccttgctg ttgatttcat aggcgcgctg ggtctggatc atgttcacca
     1681 gttcctggac cacgttcaca ttcgatgctt ctacatatcc ctgctgcaac tgtcccgcgc
     1741 cgttcaaacc cggcgtcacg acgttcgcat tacccgacga agccgtttcc gcgaacaggt
     1801 tttcgccgcg cgcgtcgagg ccggccggat tgacgaaggt cgcgagctgc agctggccga
     1861 ctgtcgtgtt ggccgcattg ccctgctgcg tcaccgtgac cgtgccgtcc gagccaatgg
     1921 tgatgccggt cgcggtcgtg gggatcgtga tggccggcgt gacctgataa ccgctcgacg
     1981 tgacgagctg gccttgcgcg ttggtctgga acgaaccgtc gcgggtgtag gcggtcgttc
     2041 cgtcgggcat cgtcacctgg aaaaagccgt tgccgttgat ggcgacgtcc ttcgagttgc
     2101 cggtctgcga caggttgccc tgcgtgtaga ggcgttcggt cgcgacctgc tgcacgccgg
     2161 tgccgatctg gctgcccgac gccagttcag tctgttgcgt ggaattcgcg cccggctggc
     2221 ggacggtctg gtacagcaag tcctcgaaca ccgcacgcga gcccttgaag ccgtttgtgc
     2281 tgacgttggc gagattgttc gaaatcacgt ccatctgcga ctgctgcgcg ttcatgccgg
     2341 ttgcggcgat gtagagagag cggttcatgt tttttatcct ggtactgcgg attaactgaa
     2401 gtcgagcagt ttgttggccg actgatcgtt cgagttggcc gaatcgagca acttggtctg
     2461 catctggaac tggcgggcgt tggtgatcat gtcgaccatc gcggacaccg cattcacgtt
     2521 gctgccttcc agcgacgcgg gcgccaccgt caccgtcgga tcggcgtcgg cgggctggtt
     2581 gtcggcggtg cgaaacaggc cgtcgtcacc gcgtgtcaaa gtccccgcat cgggattgac
     2641 gagcttcaac tggtcgaccg ttgcaatcgc cgtcggcgga tcgcccggca tcagcgcagt
     2701 caccgtcccg tccttgccga tggtgacttc cgcgcccggc ggaatcgcca tcggaccggc
     2761 attgccgagc accggcagat tgtttgccgt aaccagctgg ccgttggcgt caacgtgaag
     2821 attgccggcg cgcgtgtacg cctccccgcc atcttccgtc tgcacggaaa gccagcccgg
     2881 accctggacg gcgacatcga gaggattgcc ggtttgctgg atcggacccg gcgtgtaatc
     2941 ggcgctcggc gtggacgaca gcacgaaggt gcgcgtggtc gcatctccgg acgcgccgtt
     3001 ctcgaagttc atcggcacct gacgaaacgc ggaaagctgc gcacgaaagc ccgtcgtcga
     3061 tgcattggcg agattgttcg ccgtgacgct ctgctgctcg agcgcctgcg cggcgccgct
     3121 catcgcggta tagatcagac ggtccattta tgactctctc gtgcgcggct tggctgcggc
     3181 tcttacaggt tgatgagcgt ctggtcgacg gtttgctgcg tcttgatcgt ctgcgcgttc
     3241 gcctggtaat tgcgctgcgc cgtgatcagg tccaccagcg agctggtcag gtcgacgttc
     3301 gagttttcca ccgcgccgcc ttgcagcgag ccatggttgg tcgaacccgg caccgacact
     3361 tgcgcgagac ccgatgccgc ggtctgctgg tagttgttgc cgccaaggtc ctgcaagccg
     3421 ttctggttgg cgaacgtagc cagtgcgatc tgaccgagcg cctgggtctt gccgttcgtg
     3481 tacgtgcccg tcagcgtgcc gtccgtgccg accgaaaagc cagccagctc gctcgacgaa
     3541 aagccgtcct ggctgctgct cacaaggccg gtcgt
//
LOCUS       DATASH010000563         1629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5829641,
            whole genome shotgun sequence.
ACCESSION   DATASH010000563 DATASH010000000
VERSION     DATASH010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5829641"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="VHV99_15425"
     CDS             complement(<1..839)
                     /locus_tag="VHV99_15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HEX3636849.1"
                     /translation="MTRGFRSLNWFAVRSEHLWIDRWFGRLALRLARAGGKHVAVAWP
                     GPVWSRGAAGTPLLSWFSVPVTDAGADSFAAAINSALTVAPEHGRSAAASPEVERLMQ
                     KYARSHASTMRRSQANCPAALLENRHLTRVLLIDERVRSHGLGAIAIRNSCRTFTRML
                     QAAHTAHPQAEFWIARSDDKGNGRWLSASAGNMPPGIRHFAEHESLCAALPHVDHVYT
                     LGATEGMHALLAGVPVHVFGAPYYAGWCLTDDYLPLPNRTARPTLAALFDVVFLRFAR
                     YLNP"
     gene            complement(900..>1629)
                     /locus_tag="VHV99_15430"
     CDS             complement(900..>1629)
                     /locus_tag="VHV99_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009938575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX3636850.1"
                     /translation="ATHRHRMHTVLKHASGLITNSHATLDSLADEARRFNLPLPPTVV
                     ARLASGVTQHPLAQRPLPSPYFVMLGTIEPRKNHWFILHVWRRLVEQLGTAAPKLVVI
                     GRRGWECENVVDMLERCESLKGVVIEEAHCSDDQLHAYLQHAQALLFPSFVEGYGMPL
                     AEALALKVPVLASDLDVFHEIADDIPDYLDPLDGPGWLARIQAYARANSPERDAQLTR
                     IERFREPTWAEHFEHVDGFLETLR"
BASE COUNT          310 a          447 c          569 g          303 t
ORIGIN      
        1 ggattcagat agcgcgcgaa acgcaggaaa accacatcga ataatgcggc gagcgtagga
       61 cgggccgtgc ggtttggcaa gggcaggtag tcgtcggtca ggcaccagcc cgcgtagtag
      121 ggtgcgccga agacgtgcac cggcacaccg gccagcagcg catgcatgcc ctccgtagcg
      181 cccagcgtat agacgtgatc gacgtgaggc aacgcggcgc acaacgattc gtgctcggcg
      241 aaatggcgga tgcccggcgg catattgccg gctgacgctg aaagccagcg tccgttgcct
      301 ttgtcatccg atcgcgcgat ccagaattcg gcttgcggat gggcggtgtg cgcggcttgc
      361 agcatgcgcg tgaaggtacg gcatgagttg cgaatcgcga tcgcgccgag cccatgagaa
      421 cgtacgcgct cgtcaattaa caggacgcga gtcaggtgtc tattttcgag taacgcggcg
      481 ggacagttcg cttgcgatcg acgcatggtc gaggcatggc tgcgcgcgta cttttgcatc
      541 agccgttcga cttcgggcga ggcggcggca ctccgcccat gttcgggggc aacggtgagt
      601 gctgaattga tcgcggctgc gaaagaatcg gcgccggcat cggtcaccgg tacgctgaac
      661 caggacagga gcggcgtgcc ggccgcgccg cgcgaccata ccggaccggg ccatgcgacg
      721 gcaacgtgct tgccgccggc gcgcgcgaga cgcaatgcga ggcgcccgaa ccaccgatcg
      781 atccacagat gttccgagcg cacggcaaac cagtttagtg agcggaatcc acgcgtcatg
      841 ctgcgcagat tgattgatgg gtttgctgga gtcgactgtc cagtgacgcg ttgcttcatc
      901 tagcgcaacg tctcaagaaa accatcgaca tgctcaaaat gctctgccca tgtcggttcg
      961 cgaaagcgtt cgatgcgggt caattgtgcg tcgcgctcgg gactattcgc gcgcgcgtac
     1021 gcctgaatgc gcgcgagcca gccggggccg tcgagtgggt cgagatagtc gggaatgtcg
     1081 tcggcgattt cgtggaagac gtcgaggtcg cttgcgagta ccggtacttt gagtgcgagt
     1141 gcttcggcga gcggcatgcc atagccttcg acaaacgatg ggaacagcaa agcctgtgcg
     1201 tgctgcagat aagcgtgcag ctggtcatcg gagcagtgcg cttcttctat cacgacgcct
     1261 ttgaggcttt cgcagcgctc aagcatatcg acgacgtttt cgcattccca gccgcgtcgg
     1321 ccaatcacga cgagcttcgg tgcggcggtg ccgagttgtt cgaccaggcg gcgccatacg
     1381 tgcaggatga accagtggtt cttgcgcggc tcgatggtgc cgagcatgac gaagtatggt
     1441 gagggcaagg ggcgctgtgc gagtgggtgc tgcgtgacgc ctgaggcgag gcgcgcgact
     1501 acggtcggcg gtaagggcaa attgaagcgg cgtgcttcgt cggcgaggga atcgagtgtg
     1561 gcatgggagt tggtaatcag accgctcgcg tgcttcagga cggtatgcat gcggtgtcgg
     1621 tgcgtggcg
//
LOCUS       DATASH010000564         2349 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1477768,
            whole genome shotgun sequence.
ACCESSION   DATASH010000564 DATASH010000000
VERSION     DATASH010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1477768"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VHV99_15435"
     CDS             <1..510
                     /locus_tag="VHV99_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3636851.1"
                     /translation="AVKENGLREDLMYRLAVFPLRAPPLRERENDRELLAQHFLALLN
                     RQENASKSFSKRSIETLRTWSWPGNVRELKNAVYRAFILAEKTVELPHPHLASRVKKP
                     VTQGDSMSVWIGTPLADAQKQIILGTLKYCGGDKRRAAKALGVSLKTLYNRLSAYGDD
                     EGAEEETEQ"
     gene            886..1491
                     /locus_tag="VHV99_15440"
     CDS             886..1491
                     /locus_tag="VHV99_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636852.1"
                     /translation="MQKSMVWAGWARPIAEVSEAPGAAKRARSATTALVALAVAISSA
                     LSGCSSLYSEGATAGAGIAGAALAAKVTSNAAVATGIGLGAVAAARAGVQYSERVVHK
                     NTQDGIAKIAGPLDVGAIAPWSVIHSVPIEDDTHGRVTVSRTISAGALDCKEIVFSVD
                     QTATKNVPASSAFYVASICRDGDNWKWASAEPATERWGALQ"
     gene            1488..2108
                     /locus_tag="VHV99_15445"
     CDS             1488..2108
                     /locus_tag="VHV99_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636853.1"
                     /translation="MKARLLPHPRNESARSEPRFVEGTRAARAPRALAAVLSGSRLRL
                     VLAGALCVGASLLTSGCSSVGAASGAAAGVASGLVTSNPAIGIGIGIAVQAATDEAVA
                     HYMRVMHTDQQDMIAALAGAMPVGETRPWVVKHTLPIENGHGQVRVTRAFSSALALCK
                     DFVFSVRDGDGPNAHEDWYMASACQQDKGWKWASAEPAVERWGNLQ"
BASE COUNT          456 a          710 c          753 g          430 t
ORIGIN      
        1 gccgtgaagg aaaacggtct gcgtgaagac ctgatgtacc ggctggcggt ttttccgttg
       61 cgcgcgccgc cgttgcgcga gcgtgagaac gatcgggaac tgctcgcgca acacttcctc
      121 gccttgctga atcggcagga aaacgcaagc aagagcttca gcaaacggtc gatcgaaaca
      181 ttgcgaacct ggtcgtggcc gggcaatgtg cgtgagttga aaaacgcggt gtatcgcgcg
      241 ttcattctcg cggagaagac ggtcgaactg ccgcatccgc atctcgcgtc gcgcgtgaaa
      301 aagcccgtga cgcagggcga ctcgatgagc gtgtggatcg gcacgccgct cgccgatgcg
      361 caaaagcaga tcattctcgg cacgctcaaa tattgcggcg gcgacaagcg gcgcgcagcc
      421 aaggcgctcg gcgtgagtct gaaaacgctg tacaaccgct tgagtgcata cggcgacgac
      481 gaaggcgcgg aagaagaaac cgaacagtaa gccctgccag gcgctcgcgc ggttggttcg
      541 ctgagcgtgt gatgccggtg tgttgtcgat gtgcggggcg gctatttttt gcttcttctc
      601 ttgcgtctgt tgggccttcc ctttctgcaa aaacggccgt tttcgcgcac ctgtcgtctc
      661 ataattaatc ccttcgcacc accaatcaaa tcggttctct ctcaccagaa accgattcgt
      721 ctcgcactat cttttgcaat tgcttgcact ttcccactgc caattacaaa tggcatcctg
      781 caaaatgcgg cagcacataa tgcaacggtg agaaagacgt gcccttcatt tacacacacg
      841 tatcgcgtgt caatgaaagg cacttgcagg agcagagaca gggaaatgca aaaaagtatg
      901 gtgtgggccg gatgggcaag accaatagcc gaagtatccg aagcgcctgg agcagcgaaa
      961 cgcgcaaggt ccgctacaac ggcgctcgtc gcgttggccg tggcaatctc gtcggcattg
     1021 agcggctgta gctcgctcta ttcggaaggc gccaccgccg gcgccggtat cgcgggcgcc
     1081 gcgcttgccg ccaaagtgac cagcaacgcg gcagtggcta ccggcatcgg tctcggcgcg
     1141 gtggccgccg cgcgcgccgg ggtgcagtac tcggagcgcg tggtccataa gaatacgcag
     1201 gacggtatcg ccaaaattgc cggaccgctc gacgtcggtg cgattgcacc gtggagcgtg
     1261 atccactccg tgccgatcga agacgacaca cacggccgcg tcaccgtcag ccgcacgatc
     1321 agcgccggcg cgctcgactg caaggaaatc gtcttctcgg tcgatcagac cgccaccaag
     1381 aatgtgccgg cgagcagcgc gttctacgtc gcgtcgatct gccgtgacgg cgacaactgg
     1441 aaatgggcgt cggccgaacc tgctaccgaa cgctggggcg cgctgcaatg aaggcgcgct
     1501 tgcttcctca tccgcgcaac gaatcggctc ggtccgagcc acggttcgtc gagggcacgc
     1561 gtgcggcccg ggcaccccgc gcccttgcgg cggtactttc cggtagccgg ctgcggctcg
     1621 tgctggccgg tgcactctgc gtcggtgcca gtttgctgac gagcggctgc tcgtcggtgg
     1681 gcgctgcgag cggcgccgca gccggtgtcg cgtcaggcct cgtgacttcg aacccggcaa
     1741 tcggtatcgg catcggtatc gccgtacagg ccgcgaccga cgaagccgtc gcccactata
     1801 tgcgcgtcat gcacaccgat cagcaggaca tgatcgccgc gttggcgggg gcgatgccgg
     1861 tcggcgagac gcggccgtgg gtggtcaagc atacgctgcc gatcgagaac ggccacggcc
     1921 aggtacgcgt aacgcgcgcg ttcagctcgg cgctcgcgct gtgcaaggat ttcgtgttct
     1981 cggtgcggga cggcgacggc ccgaatgcgc acgaagactg gtacatggcg agcgcgtgcc
     2041 agcaggacaa gggctggaag tgggcatcgg cggaaccggc ggtcgagcgc tggggcaatc
     2101 tgcaataaaa aacgcaaccg ccggttacgg gaggcggcgg acgaacgggt gcgtttcccg
     2161 ctcttcggaa taaaaaagcg gcagggtctc ccccgccgcc cggattgctg accagcccca
     2221 cgttttccga tgcgtggggt ttcgttactc aggcgaggcg gatcgctgtg ttccgcttga
     2281 tcagcttcaa ccaacccgac ctggcttagg actccacgtc ttccagactg aaaatttccg
     2341 tctggtcgt
//
LOCUS       DATASH010000565         3692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3059602,
            whole genome shotgun sequence.
ACCESSION   DATASH010000565 DATASH010000000
VERSION     DATASH010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3692
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3059602"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="VHV99_15450"
     CDS             <1..723
                     /locus_tag="VHV99_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX3636854.1"
                     /translation="PPYLPLALGAGLVTPLQSAGAYSVFANGGYRINPYLISEVTDAR
                     GEPLSKAQPLTAGRDAPRTLEPRNAYIINSLLHSVATAGTGAGTNVLHRSDLQGKTGT
                     TNDAKDGWFAGYQQSLVAVAWMGYDQPKSLGSREFGAQLALPIWVEYMQRALRGVPQG
                     EPPQPDGVTAVDGEMFYTDMTPGNGFVASIGLDSANPTAGASDAIGGVGPAGMTPPAV
                     PPPSVSSNEKKQIMDLFESNKP"
     gene            1185..2036
                     /locus_tag="VHV99_15455"
     CDS             1185..2036
                     /locus_tag="VHV99_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HEX3636855.1"
                     /translation="MTGLLRVFAFLPYGVVARIGTGIGALLYRIPSGRRRVVHTNLKL
                     CFPDMSDEARERLARAHFCHVIRSYVERGPQWFGNARALARLVEVESAIDLTDMAGKP
                     TIFVGFHFVGIEAGCMMYSTRHPVASLYTPMSNLLLDGMAKRQRGRFGTEMIPRSDSV
                     RRALRVLREGKPVMLAADMDFGLLDSVFVPFFGVPACTLTSVSRMARAGNARVVPFVT
                     EVLPDYRGYKLTIFDALSDFPSDDVSVDARRITEFLETQVRRIPDQYYWVHKRFKNRP
                     VGEAGVY"
     gene            complement(2301..>3692)
                     /locus_tag="VHV99_15460"
     CDS             complement(2301..>3692)
                     /locus_tag="VHV99_15460"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEX3636856.1"
                     /translation="LTHEQKDALIIDLVRRLNELEAKLEKDSHNSSKPPSSDGPKRKP
                     KSLRGTSGARPGAQPGHKGKALKRVALPDHIRIHPVTPVCDACGQWIESSRITVRAEG
                     RQVIDLPPTRFEVTEHRVQIAQCRCGKHHSGVFPEGVSQAVQYGPQVRAAVVYLTQYQ
                     QLPVARTAQALEDLFGLHVSTGTVQHSIDEAARLLRPAVDQIRQALHEQPVVHFDESC
                     MRVGRTPHWLHIASTRALSWYGSHRKRGSEALDSFGILPGFAGIAVHDGWRPYAGYEC
                     GHALCNAHHLRELVFVLESTQQTWAQQMIDLLCQAKREVDLSVASGNTALDQSRQRYY
                     ARRSRTLIAQACRLNPQQAREPTRQERRGRIRQSFTCNLLRRLQKHAREVWRFITDHR
                     VPFDNNQAERDIRMPKLKQKISGCFRSESGMQAFCTIRSYLATLRKQNRSLIDALASS
                     FAGIVDSPLPAAE"
BASE COUNT          595 a         1051 c         1190 g          856 t
ORIGIN      
        1 ccgccgtatc tgccgctggc ccttggcgcg gggcttgtca cgccgctgca atcggcgggc
       61 gcgtattcgg tgttcgcgaa cggcggctac cggatcaatc cgtatctgat cagcgaagtc
      121 accgacgcgc gcggcgagcc gctttcgaaa gcgcaaccgt tgaccgccgg tcgcgatgca
      181 ccgcgtacgc tcgaaccgcg taacgcatac atcatcaaca gcctgctgca ttcggttgcc
      241 accgccggga cgggtgcggg caccaatgtg ctgcatcgtt cggacttgca gggcaagacg
      301 gggacgacca acgacgcgaa ggacggctgg ttcgccggtt atcagcaatc gctggtggcg
      361 gtggcgtgga tgggttacga ccagccgaag agcctcggca gccgcgaatt cggtgcgcag
      421 cttgcgctgc cgatctgggt cgagtacatg cagcgcgcgc tgcgcggcgt gccgcaaggc
      481 gagccgccgc agccggacgg cgtgaccgcg gtcgacggcg aaatgttcta taccgacatg
      541 acgccgggca atggtttcgt ggccagcatc ggtctcgatt cggcgaatcc cactgccggc
      601 gcgagcgacg caattggcgg cgtcgggccg gcgggtatga cgccgccggc ggtgccgccg
      661 ccgagcgtgt cgtcgaatga aaagaaacag atcatggatc tgttcgagtc gaacaagcct
      721 tgatgtcgct cgggacttcc ttcgaggggg ccgccaaagg ggcccgcttg ggggcgcagt
      781 gagcgtgtgc ggccggcttc ggctgcatgc ttgcgttttt gaaagggcag ggaaagcgcc
      841 gccctttcac agatttcgtg atgttcccgg gacagccgtt ccagcagatt ttgtgcccgc
      901 cttccgcagt gtgttttaat cacgcctgcg gtgtctcaat attaggtcat acacggacgc
      961 tcgattccta agcggtttcg gtcgccggca ccgctacgtt tcgccgcgag cgagacgtcc
     1021 accttcgcac ctctcgcgcc gcgcgagacg tctttgacgc ctttgacgcg cgcgaatgcc
     1081 acttcgacca tcagcaatgc gatgttgctc cggccgtggt gttacacacc cagcatccac
     1141 caaggtctcc agaactcgtg aaaagtcgaa tactcatctg tttgatgaca ggtctgctac
     1201 gcgtgtttgc ttttttgccg tatggcgtgg tcgcgcgcat cggcaccggc atcggtgcgt
     1261 tgctgtaccg gattccgagc ggccgccggc gcgtcgtgca taccaatctg aaattgtgtt
     1321 ttcccgacat gagcgatgag gcgcgcgaac gccttgcgcg cgcgcatttc tgtcatgtga
     1381 tccgcagtta cgtcgagcgt gggccgcaat ggttcggcaa tgcgcgggcg ctggcgcgtc
     1441 ttgtcgaggt ggagagcgcg attgatctca ctgacatggc gggcaagccg acgatttttg
     1501 tcggcttcca ttttgtcggt attgaggcgg gctgcatgat gtattcgacg cggcacccgg
     1561 ttgcgtcgct gtacacgccg atgtcgaacc ttttgctcga cgggatggcc aagcggcagc
     1621 gcgggcgctt cggcacggag atgattccgc gcagcgatag cgtgcggcgg gctttgcgtg
     1681 ttttgcgtga gggtaagccg gtcatgctgg ctgctgatat ggactttggg ttgctggatt
     1741 cggtgtttgt gccgtttttt ggcgtgccgg cttgcacgct gacttctgtt tcgcggatgg
     1801 cgcgcgcggg gaatgctcgt gttgtgccgt ttgtgactga ggtcctgccg gattatcgtg
     1861 ggtataagct gactattttt gatgcgttga gcgattttcc ttcggatgat gtttctgttg
     1921 atgctcggcg gattactgag tttcttgaga cgcaggtgcg acggattcct gatcaatatt
     1981 attgggtgca taagaggttt aagaatcggc ctgtgggtga ggcgggggtt tattgacgtt
     2041 ttttttgcgc ggggcgcgag ggtgggggtt tttgtttgcg tggcggttga ttgtctgttt
     2101 gtctgtggcg ttgggctttt cctgatctgg gttttttttg gcctttcctt gaattgttta
     2161 cctgcggcgg tggggttggt tgtttgccta cggggttggc ctttccttga tctggctctt
     2221 tggcctttcc ttgatttgtt ggtggtctat tagcgttgcc cctgtgcggg gcggcactta
     2281 ctttctttgc cgcggcgtaa ctattcagcg gcaggcaaag gcgaatcgac gattccggca
     2341 aagctggagg ccagtgcatc gatcagcgaa cggttctgct tgcgcagggt ggcaaggtac
     2401 gatcggattg tgcagaacgc ctgcatgcct gattccgagc ggaagcagcc cgagattttc
     2461 tgtttgagtt taggcatgcg gatatcgcgt tcggcctggt tgttgtcgaa cgggacccgg
     2521 tggtcggtaa tgaaccgcca cacctcacgt gcatgcttct gaaggcgtct gagcaggttg
     2581 caggtaaagc tttgcctgat cctgccgcga cgctcctggc gcgtgggctc acgggcctgc
     2641 tgcggattga gcctgcacgc ctgggcaatc agcgtacggc tgcgccgtgc gtaatagcgt
     2701 tggcgcgatt gatccagcgc ggtgttgcct gaggccacac tgaggtcaac ctcacgcttg
     2761 gcctggcaaa gcagatcgat catctgctgg gcccaggtct gctgcgtgga ttccagtaca
     2821 aacaccagtt cgcgcagatg gtgggcattg cacagcgcat ggccacactc atagccggcg
     2881 tacggcctcc agccgtcatg caccgcgatt cccgcaaagc cgggcagaat gccgaagctg
     2941 tccagcgcct cgctaccgcg cttgcggtgt gagccgtacc agctcaacgc gcgcgtcgag
     3001 gcaatatgca gccagtgagg cgtgcgtccc acgcgcatac agctctcatc gaaatgcact
     3061 actggctgct cgtgtagcgc ctgcctgatc tgatcaaccg ccggcctcag caaccgggca
     3121 gcctcgtcta tgctgtgctg tacggttccc gtactcacat gcagaccaaa caggtcctcc
     3181 agcgcctggg cagtgcgcgc aaccggcaac tgctggtatt gcgtcagata gacgaccgcg
     3241 gcgcgcacct gaggaccata ctgcaccgcc tggctcacgc cctcgggaaa cacgcccgaa
     3301 tggtgcttgc cgcagcggca ctgcgcgatc tggacgcgat gctcggtcac ctcaaagcgc
     3361 gtaggcggca gatcgattac ctgacggcct tcagcccgca cggtgatccg gctggattcg
     3421 atccactgtc cacatgcgtc gcataccggc gttaccgggt gaatcctgat gtgatcgggc
     3481 agcgctacac gtttgagcgc cttgccctta tgccccggtt gcgcgccagg ccgcgcgccg
     3541 ctcgtgccac gcaatgactt cggcttgcgc ttcggaccat cgcttgacgg cggcttgctg
     3601 gagttatgac tgtccttctc cagtttcgct tcgagctcgt tcagacgtct caccagatca
     3661 atgatgagcg cgtccttctg ttcgtgcgtc ag
//
LOCUS       DATASH010000566         4408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1281147,
            whole genome shotgun sequence.
ACCESSION   DATASH010000566 DATASH010000000
VERSION     DATASH010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4408
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1281147"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1826)
                     /gene="ilvD"
                     /locus_tag="VHV99_15465"
     CDS             complement(<1..1826)
                     /gene="ilvD"
                     /locus_tag="VHV99_15465"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX3636857.1"
                     /translation="MPHYRSRTSTHGRNMAGARALWRATGMKDDDFGKPIIAVVNSFT
                     QFVPGHVHLRDLGALVAKEIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMAAMRLNIPVVFVSGGPMEAGKVKSPTDGQ
                     VIAKIDLIDAMIKAADPKISDAEVAEIERSACPTCGSCSGMFTANSMNCLTEAIGLAL
                     PGNGTIVATHAWRKGLFEQAGRLVVDLCRRYYQEEDTSVLPRSIASKQAFENAMALDV
                     AMGGSTNTVLHLLAAAQEAGVDFTMSDIDRISRKVPCLCKAAPATDKYHIEDVHRAGG
                     ILGILGELARAELLDLSCGNVHSGTLGNAIARWDVAGGAGEEAQKFFRAAPGGIPTTV
                     AFSQEATFASLDTDRKTGCIRSKQDAYSKDGGLAVLYGNLAEKGCIVKTAGVDESQWI
                     FSGRARVFESQDDAVEAILGDKVVAGDVVVIRYEGPKGGPGMQEMLYPTSYLKSKGLG
                     KTCALFTDGRFSGGSSGLVIGHASPEAAEGGTIGLVEEGDVIEIDIPHRKMHLVVSDE
                     ALARRREAMEARGDKAWMPVDRERVVSQALQAYAALATSADRG"
     gene            2086..3222
                     /gene="ribBA"
                     /locus_tag="VHV99_15470"
     CDS             2086..3222
                     /gene="ribBA"
                     /locus_tag="VHV99_15470"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEX3636858.1"
                     /translation="MTLASTQEIIAELKAGRMVILVDEEDRENEGDLVIAAEFVTPEA
                     INFMARYGRGLICLTLTQERCKTLNLPLMTYRNGTQYGTAFTVSIEAAEGVTTGISAA
                     DRARTIAAAVAPDAKAEHIVQPGHVFPIMAQPGGVLVRAGHTEAGCDFTALAGLTPAA
                     VICEVIKDDGTMARLPDLMEFAKEHDLKIGTIADLIHYRSRTESIVERICERTMQTAH
                     GAFRAVMYLDQPSGQPHIALVRGTPNPEHDTPVRVHEPLSMLDLLEVGKSTHSWTLDA
                     AMKEIAQRDLGVIVLLNCGDSKEHLVDVFKAFDSKEKAEALKRRPVDFKTYGIGAQIL
                     RELGVGKMQVLSNPRKLGSMSGYGLEVTGFVPMPGSTAQAPQQG"
     gene            3273..3779
                     /gene="ribH"
                     /locus_tag="VHV99_15475"
     CDS             3273..3779
                     /gene="ribH"
                     /locus_tag="VHV99_15475"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012400029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX3636859.1"
                     /translation="MEIGQYQPNLDGDGLRIGIVQARFNEPVCNGLADSCIEELERLG
                     VTGEDVLLVTVPGALEIPLALQKLAESAQFDALIALGAVIRGETYHFELVSNESGAGI
                     TRIGLDFGIPVANAVLTTENDEQAVARMTEKGRDAARVAVEMANLAVALEQLGGDDEE
                     EDEEEEEA"
     gene            3776..4210
                     /gene="nusB"
                     /locus_tag="VHV99_15480"
     CDS             3776..4210
                     /gene="nusB"
                     /locus_tag="VHV99_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX3636860.1"
                     /translation="MKSARRRSRELATQGLYQWLLSGSPGGEIDAQLRGAQGFDKADH
                     EHLDAILHGVIRDSEALSADIAPCLDRPIDQLSPVERAVLLVAAFELKNHVDIPYRVV
                     INEAVELAKTFGGADGYKYVNGVLDKLSAQLRAAETQAARKH"
BASE COUNT          822 a         1425 c         1400 g          761 t
ORIGIN      
        1 ccgcggtcgg cggatgtggc gagcgccgca taagcctgca acgcctgcga taccacgcgt
       61 tcacgatcca ccggcatcca ggccttgtcg ccgcgcgctt ccatcgcttc gcggcggcgt
      121 gccaatgctt cgtccgacac caccagatgc atcttgcggt gaggaatgtc gatttcgatc
      181 acatcgccct cttccaccag accgatcgtg ccgccttcgg cggcttcggg cgacgcgtgt
      241 ccgatcacca gccccgacga accgccggag aaacggccgt ccgtgaacag cgcgcaggtc
      301 ttgcccagac ccttggattt cagatacgac gtggggtaaa gcatttcctg catgccgggg
      361 ccgcccttgg ggccttcgta acggatcacg accacgtcgc ccgccaccac cttgtcgccc
      421 agaatggctt cgacggcgtc gtcctggctc tcgaacacgc gtgcacgccc ggagaagatc
      481 cattgcgatt cgtcgacacc cgcggtcttg acgatacagc ccttctccgc gaggttgccg
      541 tacagcacgg cgaggccgcc gtctttcgaa tacgcgtcct gcttgctgcg gatacagccg
      601 gtcttgcggt cggtatcgag cgacgcgaag gtggcttcct gactgaatgc aaccgtggtc
      661 ggaataccgc ccggagcggc gcggaagaat ttctgcgctt cttcgcccgc gccgccggcc
      721 acgtcccacc tggcaatcgc attgcccagc gtgccgctat gcacgttgcc gcacgacagg
      781 tcgagcagct ccgcccgcgc cagctcaccg agaatgccca ggatgccgcc ggcacggtgc
      841 acgtcttcga tatggtattt gtccgttgcg ggcgccgcct tgcacaggca cggcactttg
      901 cgcgagatgc ggtcgatgtc ggacatcgtg aaatcgacgc cggcttcctg tgcggctgcc
      961 agcaagtgca gcacggtatt ggtcgaaccg cccatcgcga cgtcgagcgc catggcgttc
     1021 tcgaacgcct gcttgctggc gatgctgcgc ggcaggaccg acgtgtcttc ttcctgatag
     1081 tagcggcggc acagatccac cacgagacgg ccggcctgtt cgaacaggcc cttgcgccat
     1141 gcgtgcgtgg cgacgatcgt gccgttgccc ggcagcgcga ggccgatcgc ctcggtcagg
     1201 cagttcatcg agttcgcggt gaacatgccc gagcaggaac cgcacgtcgg gcaggcgctg
     1261 cgctcgattt cggccacttc ggcgtcgctg atcttcgggt cggcggcctt gatcattgcg
     1321 tcgatcaggt cgatcttggc gatcacctgg ccgtcagtcg gcgatttgac cttgcccgct
     1381 tccatcggac cgccggacac gaacaccacg gggatgttca ggcgcatcgc ggccatcagc
     1441 atgcccgggg tgatcttgtc gcaattggag atgcagacca tggcgtcggc gcaatgcgcg
     1501 ttgaccatgt attcgaccga atcggcgatc agttcgcgcg acggcagcga atacagcatg
     1561 ccgccgtggc ccatggcgat gccgtcgtcc accgcgatgg tgttgaattc cttggccaca
     1621 ccgccggccg cttcgatttc cttcgcgacc agcgcgccca ggtcgcgcag atgcacatgg
     1681 ccaggcacga actgggtgaa cgagttcacc acggcgatga tgggcttgcc gaaatcgtcg
     1741 tctttcattc cggtggcgcg ccacaaggcg cgggcgccgg ccatgttgcg gccgtgagtc
     1801 gatgtgcgtg agcgatagtg cggcatgatc tgtctcgaac gtttagcggg gaaattgatg
     1861 tctggaaacg gccttcgcgg gcgcggcgaa ttatagcaac gtcgcttgtc gacccgagtt
     1921 ggcgcttcag cgcctgctcg cggcccatgc aaagctgctg aaccgccgcc ggacggcccg
     1981 ggcatggtcc ggacgactcg ccaaacggcc tatgccgtcc ggcgcaggcg acttgggagc
     2041 gcgcgtggcg taaaatacgc gctttcttcg aaaaactccg ccaacatgac gctcgcctcc
     2101 actcaagaga tcattgccga actgaaagca ggccggatgg tgatcctcgt cgacgaagaa
     2161 gaccgcgaaa acgagggcga cctcgtgatc gccgccgaat tcgtcacgcc ggaagcgatc
     2221 aacttcatgg ctcgctacgg ccgcggcctg atctgcctta cgctcacgca ggaacgctgc
     2281 aagacgctga acctgccgct catgacctat cgcaacggca cgcagtacgg caccgcgttc
     2341 acggtcagca tcgaggcggc ggaaggcgtc accaccggca tctcggcggc cgaccgcgcc
     2401 cgcacgatcg ccgcggcggt cgcgccggac gcgaaagccg aacacatcgt ccagccgggc
     2461 cacgtgttcc cgatcatggc gcaaccgggc ggcgtgctgg tgcgcgccgg tcacaccgag
     2521 gccggctgcg acttcaccgc gctggcaggc ctcacgccgg ctgcggtgat ctgcgaagtc
     2581 atcaaggacg acggcacgat ggcacgcctg ccggacctga tggagttcgc caaagagcac
     2641 gatctgaaaa tcggcacgat cgcggatctg atccactacc gcagccgtac tgaatcgatc
     2701 gtcgagcgca tttgcgaacg caccatgcag accgcgcacg gcgcgtttcg cgcggtaatg
     2761 tacctcgacc agccgagcgg ccagccgcat atcgcgctgg tgcgcggcac gccgaacccg
     2821 gaacacgaca cgccggtgcg cgtgcacgag ccgctgtcca tgctggatct gctcgaagtc
     2881 ggcaagtcca ctcattcgtg gacgctggat gcggcgatga aagagatcgc ccaacgcgac
     2941 ctcggtgtga tcgtgctgct gaattgtggc gattcgaagg aacatctggt cgacgtgttc
     3001 aaggcgttcg actcgaaaga gaaagccgag gcgctcaaac gccggccggt cgacttcaag
     3061 acgtacggca tcggcgcgca gattctgcgt gagctgggtg tcggcaagat gcaggtgctg
     3121 tcgaacccgc gcaagctggg cagcatgtcg ggctacggcc tcgaagtcac cggcttcgta
     3181 cccatgccgg gcagcaccgc acaggctccg caacaaggct gatccgacca ttcacgcgct
     3241 taagcgccaa ctcaaaacac tacggaattc tcatggaaat cggacaatac caaccgaatc
     3301 tcgacggcga cggactgcgt atcggcatcg tccaggcgcg ttttaacgaa cccgtctgca
     3361 acggtctcgc ggattcgtgc atcgaagaac tcgaacgcct cggcgtgacc ggcgaagacg
     3421 tgctgctggt caccgtgccg ggcgcgctgg aaatcccgct ggcgctgcaa aagctcgccg
     3481 aaagcgcgca attcgacgca ctgatcgcgc tgggcgcggt gattcgcggc gagacgtacc
     3541 acttcgagct ggtgtcgaat gaaagtggcg cgggcatcac gcgtatcggc ctcgacttcg
     3601 gcattccggt cgcgaacgcc gtgctgacca cggaaaacga cgagcaagcc gtcgcgcgca
     3661 tgaccgagaa gggtcgtgac gcagcgcgcg tagctgtcga aatggcaaac ctcgccgtcg
     3721 cgctcgaaca actcggcggt gacgacgaag aagaagacga agaagaggaa gaggcatgaa
     3781 gagcgcacgc cgacgctccc gcgaactggc cacgcagggg ctttatcagt ggttgctgtc
     3841 gggctcgccc ggcggtgaaa tcgacgcgca gctgcgcggc gcgcaaggct tcgacaaagc
     3901 cgaccacgaa catctggacg ccatcctgca cggcgtgatc cgcgattcgg aagcgctttc
     3961 cgcggatatc gcaccgtgcc tcgaccgacc gatcgaccag ctttcgccgg ttgaacgtgc
     4021 cgtgttgctg gtcgccgcgt tcgaactgaa gaatcacgtc gatatcccct atcgcgtggt
     4081 catcaacgaa gcggtggagc tcgccaagac gtttggcggc gcagacggct acaagtacgt
     4141 gaacggtgtg ctggacaagc tgtcggcgca actgcgcgct gctgaaacac aggcggctcg
     4201 caagcattga ggtgggacgc gtgacctggt catgcgcctc gcgctaaacg ttgacccgcc
     4261 gggtgccggc ctgtgaagtg cggtgccggt gggacgccag cggctgggtt cggcgacggc
     4321 tggtttccgt ggccaccgta ttgccagcgg ctggctcgtc ggcagccgga tcgccgggcg
     4381 ccggccggtt gatccccaaa ctgaacaa
//
LOCUS       DATASH010000567         5566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1875318,
            whole genome shotgun sequence.
ACCESSION   DATASH010000567 DATASH010000000
VERSION     DATASH010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5566
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1875318"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..416
                     /locus_tag="VHV99_15485"
     CDS             30..416
                     /locus_tag="VHV99_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4148 domain-containing protein"
                     /protein_id="HEX3636861.1"
                     /translation="MKNLSYLLVTIGAFASPVVSFAQSTEPLTRAQVRAELIRLEQAG
                     YHVSIGDYTTYPAGIQAAESKIAAQDGQQTANKSAPESQQTANNAVGGTTLNGTSAAG
                     SYPHLPHPSLSSCVGPASYCNLFFGN"
     gene            complement(741..1754)
                     /locus_tag="VHV99_15490"
     CDS             complement(741..1754)
                     /locus_tag="VHV99_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018310047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX3636862.1"
                     /translation="MNYSGIDLHSNNSVVSVIDETDRVVAERRLPNDLAKILAFFAPW
                     QAGLAGVVVESTYNWYWLVDGLQAASFVVHLANTTAIKKYDGLKHSGDETDARYLAHL
                     LRLGILPTGTILPAKHREVRDLARKRMRLVHSCTSHILAVENITARQSGTRITSNQVK
                     RLDEVAADQMGLPENVALAVRANVAVITTLREQIAIVERRVQEEVAPHPGYALLTTVP
                     GIGQTLATVIVLETGTIERFASAGNFASYARCVDSQRMSNGKKKGEGNTRNGNPYLCW
                     AFIEAANFAMRFNAEARLFYERKKARTNPVIARKALAHKLARACFHMLKERKPFDVTR
                     CFA"
     gene            1963..3092
                     /locus_tag="VHV99_15495"
                     /pseudo
     CDS             1963..3092
                     /locus_tag="VHV99_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007739193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            complement(3224..4624)
                     /locus_tag="VHV99_15500"
     CDS             complement(3224..4624)
                     /locus_tag="VHV99_15500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006757738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HEX3636863.1"
                     /translation="MSFVIVLAALAFLMFAAYRGYSVILFAPIAALGAVLLTDPGAVA
                     PVFSGIFMEKMVGFVKLYFPVFLLGAVFGKVIELSGFSESIVAAAIRYIGRSRANAVI
                     VAVCALLTYGGVSLFVVVFAVYPFAAELYRQSNIPKRLMPGAIALGAFSFTMDSLPGT
                     PQIQNIIPTTFFKTTSWAAPTLGVIGSLFIIVVGLTYLEWRRRAAMATGEGYGTDLVN
                     EPERVESKQLPHPLLAVAPLVLVGVANFLLTHWIPDWYGASYTIAPDILPGIHAPVTT
                     TIKSVVAIWAVEGALLLGIVMVVVTAFGRVRERFAVGTKAAVAGALLASLNTASEYGF
                     GGVIAALPGFLVVSDALKSIPNPLVNAAVSVSSLAGITGSASGGMSIALAAMSDTFIK
                     GAEAAHIPMEVLHRVVAMASGGMDTLPHNGAVITLLAVTGLTHRQSYRDIFAVTVIKT
                     LAVFFVIAVYYMTGLV"
     gene            4937..>5566
                     /locus_tag="VHV99_15505"
     CDS             4937..>5566
                     /locus_tag="VHV99_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX3636864.1"
                     /translation="MMNDWAGLPATYGDVLRRAMDSLFRTFENSSEGTFIVDAESRVV
                     WINKRYAARFGFSDPEQAIGRDCEEVIPNSLMREVVNTGKPILLDILETDREPLVVTR
                     LPLKDDAGKTVGAVGFALFDELKALTPLFSHYSRVQQELIATRQSLAQARRAKYTFSS
                     FVGTSAASLEVKRQARRAAQLESPVLLLGETGTGKELLAHAIHGGSARAN"
BASE COUNT         1152 a         1744 c         1657 g         1013 t
ORIGIN      
        1 ccccattcaa cgactcacgg agcttcatca tgaaaaacct ttcatacctc ctcgtcacca
       61 tcggtgcatt cgccagccca gtcgtcagct tcgctcaaag caccgagccc ctcactcgcg
      121 cgcaagtgcg cgcggaactg atccgtctcg agcaggccgg ctatcatgtc agcatcggcg
      181 actacacaac atatcctgct gggattcagg cagctgaatc gaagatcgcc gcacaggatg
      241 gccagcagac ggcaaacaaa tcagcgccag aaagccagca gacggccaac aacgccgttg
      301 gcggcacgac gcttaacggc acctcagccg ccggcagtta cccacatctg ccgcacccct
      361 cgctatcgtc gtgcgtcggc cccgcaagtt actgcaatct tttcttcggt aactaaccgt
      421 acgaagcgta cctgacactg ccccgacgat ccggctaaca tcccggcatc gtctgggagc
      481 agcatcaccg gcggcgtccg aatcgggttg cctgtcgccc tggtggcttt gccaccccag
      541 aagaactttg gcaccgcaac gcgtccatcg ggtttgcaca atacgcttgg ttgccgaacg
      601 tccggcacct atccagggct ggctcgcgtt gcaggcgatc cgttttgccg cacatcgggg
      661 gacggcggca cggcatccca ttcaatcggg tggctgctcg ccagtcgacg attagggctt
      721 cccggtcgcc gtcgcaactt ttacgcgaag cagcgtgtca catcaaatgg ctttcgctcc
      781 ttgagcatgt gaaagcacgc acgcgccagc ttgtgtgcca gtgccttgcg cgcgattacc
      841 ggattggtcc gggctttctt gcgctcatag aacagcctgg cttcggcgtt aaagcgcatc
      901 gcgaagttgg ccgcctcgat gaatgcccag cacaggtatg gatttccgtt cctggtattg
      961 ccttcgccct tcttcttgcc gttgctcatg cgctggctgt ccacacaccg tgcgtaggag
     1021 gcgaagttgc cagcgctggc gaaacgttcg atggtcccgg tttccagcac gatcacggtg
     1081 gcaagcgttt ggccaatgcc gggcaccgtg gtcagcaggg cgtagcccgg atgtggcgcg
     1141 acttcttcct gaacacgcct ttccacgata gcgatctgtt cgcgaagcgt cgtgatgacc
     1201 gcgacgttcg cccgaaccgc gagcgctacg ttctctggca ggcccatctg atcggcagcc
     1261 acttcgtcga gtcgcttgac ctgattgctc gtgattcgcg tgccggactg acgcgctgta
     1321 atgttctcga cggcgagaat gtggctggtg cagctgtgta ccagtcgcat gcgtttgcgg
     1381 gccaggtctc gtacctcacg gtgcttcgct ggcaggatgg tgccagtggg aaggatcccc
     1441 aggcgcagca ggtgggccag gtagcgcgcg tcggtctcat cgccactgtg cttgagcccg
     1501 tcgtatttct tgatcgcggt ggtgttggcg agatggacca cgaagctggc ggcctgcagg
     1561 ccgtcgacaa gccagtacca gttatacgtc gattcgacca cgacaccggc cagcccggct
     1621 tgccacgggg caaagaaagc cagaatcttt gccaggtcgt tcggcagccg cctttcggcg
     1681 accacacggt cggtttcgtc gatcacgctc accacgctgt tgttcgaatg caggtcaatg
     1741 ccgctatagt tcatgatgac gtgcgacacg taacgctgtc cgccggacgt aaatcggcaa
     1801 cgtagttgac ggcgaagctc tttcttgtct tgtcacattc gggatctttc gaaatgtgac
     1861 ctgctgtacg accagcagtt gctgtataga ccggagacat agctgacaga tgtgcggaga
     1921 catggttgac acttccgggt attaaaacgc ccggtcccga ccatgccctg gaacgcaaga
     1981 gacaccatga gcctccgaca ggaatttgtt catctggcca gtcaggacac gctgacgatc
     2041 accgagttgt gccagcgctt taacatcagc cggcagaccg gctacaaatg gctcaggcgc
     2101 ggcgagaacg cgctgtcaga tcagtcccgg cgccctgcca gcagcccctc gaagacaccc
     2161 gccgcgatgg agcaggaagt ggtgcggcta cgtcaggccc atccgcgctg gggcgggtgc
     2221 aagatcaggt ggcgcctgca ggatctgcgc tttgaggcgg tgccgcagtc cagcaccgtg
     2281 accgacatcc tgcaccggca caacctgatc ctgccggccg attcagcgat gagcgaaccc
     2341 tggaagcgct ttgagcacga gcagcccaac gtgctctggc agatggactt caaggggcac
     2401 ttcgagaccc tccagaagga gcgttgcagt cccctgacgg tactggacga ccattcgcgc
     2461 ttcagcatcc tgctgcgcgc ctgtggaccc accgataccg caaccgtgca ggcggggtta
     2521 cgggaggcgt tcgggcacta cggcctgccg ttgcgcatca acaccgacaa ggctcgccgt
     2581 ggggcgcgcc cggcagtccg gggcagttca ccgagctggc cgtctggctg atccagctgg
     2641 gtatccgcat cagctacagc cggccttacc acccgcagac caacggcaag gacgaacggt
     2701 ttcaccgtac gctgaaggct gaagtactga acggacgaag cttcgccacc cagcagcacg
     2761 tgcagcagga gctggaccgc tggcgcacca tctacaactg cgagcgcccg cacgaggcga
     2821 tcggcatgaa cacaccgatc agccgttacc agccgagccc ccgggcgtat ccatcgatcc
     2881 tgcgggagcc ggagtacggc ccggatgatg tggtactgcg ggtcaagtcc gatggccggc
     2941 tacgctttga aggacggaaa cttaaagtct cgaatgcact ttatgggctg ccggttgcgg
     3001 cacgcgcaaa gcctggcgaa gacggcgtat ttgaattctg gtttgcccat catcgcatcc
     3061 tcacccttga cctgaggagc aacaatccct gaacataaag tgtcaacgat gtccccgcac
     3121 gtttgtcaat catgtccccg gtctatacac gtccacaaca taagtttgtc ggcaatgaca
     3181 gactttagtt gtgacagcac agccgtctgt cagagcggca aagttaaacc agccccgtca
     3241 tgtagtacac cgcgatcacg aagaaaaccg ccagcgtctt gatgaccgtc accgcgaaaa
     3301 tatcgcggta cgactgacgg tgcgtcaaac ccgtcaccgc cagcaacgtg atcaccgcgc
     3361 cgttatgcgg caacgtgtcc atgccgccgc tcgccatggc gacgacccgg tgcagcactt
     3421 ccatcggaat gtgcgcggct tccgcaccct tgatgaacgt gtcggacatg gccgcgagcg
     3481 cgatgctcat gccacccgat gccgaacccg taatgcccgc cagcgagctc accgacacgg
     3541 ccgcgttgac gagcggattc ggaatactct tcagcgcatc gctgaccacc aggaagcccg
     3601 gcaaagccgc aattacgccg ccgaagccat attccgacgc cgtattcaac gaagccagca
     3661 acgcgccggc caccgcagcc ttggtgccga ccgcgaagcg ttcgcgcacg cggccgaacg
     3721 ccgtcaccac gaccatcacg ataccgagca gcagtgcgcc ttcaacggcc cagatcgcga
     3781 cgacgctttt gatcgtcgtc gtgaccggtg catggatgcc cggcaggatg tccggcgcga
     3841 tggtgtacga cgcgccgtac cagtccggaa tccagtgcgt gagcaggaag ttcgccacgc
     3901 cgaccagcac cagcggcgcg accgcgagca gcggatgcgg cagttgcttc gattcgacgc
     3961 gttccggttc gttgacgaga tccgtgccgt agccttcgcc ggtcgccatc gccgcgcggc
     4021 ggcgccattc caggtaggtg aggccaacca cgatgatgaa cagcgagccg atcacgccga
     4081 gcgtgggcgc ggcccaggag gtcgtcttga agaaggtggt cgggatgatg ttctggatct
     4141 gcggcgtgcc cggcagcgaa tccatcgtga acgagaacgc gccgagcgcg attgcacccg
     4201 gcatcaggcg tttcggaata ttgctctggc ggtacagctc cgcggcgaac ggatacaccg
     4261 cgaacaccac cacgaacaag gacacgccgc cataggtgag cagcgcgcac accgcgacga
     4321 tcaccgcatt cgcacgtgaa cggccgatat aacggatggc cgcggcaacg atcgattccg
     4381 agaagccgga cagttcgatg accttgccga acaccgcgcc gagcagaaac accgggaaat
     4441 aaagtttgac gaagccgacc atcttctcca taaagatgcc ggagaacacc ggcgcgacgg
     4501 cgcccggatc ggtgagcagc accgcgccga gggcggcgat cggtgcgaac agaatgacgc
     4561 tgtagccgcg atatgcggca aacatcagaa acgccagcgc ggcgaggacg atgacaaagg
     4621 acattgagtc tccaaagctt gatagttcta cgtgtacgcc gtcccgatgg tgcgacggcc
     4681 cggcgcccgc aaacgcagat tccgtgccat gcgcgccgta gcggcctgcc cgttgatttc
     4741 acaggccaaa gttcgaaacg tgctgcggcc gattgtaccc aaacgtctct gaaacgggac
     4801 gcaaatcagc tgaacatagg gcgaaccgag attaaaccct tatccggaga gcttgctggc
     4861 gatctcgtcg atgagataag cttgtctacg aaatgagaca tgcataaata aaacgatagc
     4921 cggagacagc gacaccatga tgaacgactg ggcgggtctt cctgccactt acggcgatgt
     4981 acttcgccgt gcgatggatt cgctcttccg tacgttcgaa aattccagtg aaggcacctt
     5041 catcgtcgac gccgagtcgc gcgtcgtctg gatcaacaag cgctacgcgg cgcgcttcgg
     5101 tttttcggac ccggagcagg cgatcggccg ggattgcgag gaggtgattc ccaacagcct
     5161 gatgcgcgag gttgtgaaca ccggcaagcc gattctgctc gacatcctcg aaacggaccg
     5221 cgagccgctt gtcgtgaccc ggctgccgct gaaagacgat gcgggcaaaa cggtcggcgc
     5281 ggtgggtttc gcgttgttcg acgaattgaa ggcgctgacg ccgctttttt cccattactc
     5341 gcgcgtgcag caagagttga tcgcgacgcg tcagtcgctc gcgcaagcaa gacgggcgaa
     5401 atacacgttc agcagtttcg tcggtacgag cgcggccagt ctcgaagtga agcgccaggc
     5461 gcgccgcgcc gcgcaactcg agtcgccggt gctgttgctc ggcgaaaccg gcacgggtaa
     5521 ggaactgctg gcgcatgcca ttcacggcgg gtcggcgcga gcaaat
//
LOCUS       DATASH010000568         1615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3455529,
            whole genome shotgun sequence.
ACCESSION   DATASH010000568 DATASH010000000
VERSION     DATASH010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3455529"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="VHV99_15510"
     CDS             complement(<1..986)
                     /locus_tag="VHV99_15510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003103909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HEX3636865.1"
                     /translation="MKKLTTVFGAPVVDNQNIKTAGPRGPALLEDVWFLEKLAHFDRE
                     VIPERRMHAKGSGAFGTFTVTHDITQYTRAKIFSEIGKKTEMFARFSTVAGERGAADA
                     ERDIRGFALKFYTEEGNWDLVGNNTPVFFLRDPLKFPDLNHAIKRDPRTGLRSAESNW
                     DFWTQLPEALHQVTIVMSDRGIPKTFRHMHGFGSHTFSFINAANERFWVKFHFRSQQG
                     IENLTDAEAEALVGRDRETHHRDLYEAIERQEFPRWTLYVQVMPEKEAGSYPINPFDL
                     TKVWPKRDFPLIEVGVMELNRNADNHFADVEQAAFAPANVVPGISFSPDKML"
     gene            complement(1027..>1615)
                     /locus_tag="VHV99_15515"
     CDS             complement(1027..>1615)
                     /locus_tag="VHV99_15515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010118050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HEX3636866.1"
                     /translation="FGALSGERKGLKYDAELLYLGAMFHDMGLMPAYSSEGYRFEVDG
                     ANAARDFMTQHGMNDYDIEQVWLSIALHTTPGVPEHLKPVVALVTAGVEMDVLGMAYH
                     EFSDEQRIQVVAAHPREETFKNGIIDAFAQGTIRKPETTFGNVKADVLELRDPSYKRL
                     NFCQIILGSAWDDSKAGHAHQQGCGCGTDSAAKGN"
BASE COUNT          297 a          486 c          478 g          354 t
ORIGIN      
        1 tgcaacatct tgtcgggcga gaagctgatg ccaggcacca cgttggcagg agcaaaagcg
       61 gcctgctcga cgtcggcgaa atgattgtct gcgttacggt tcaattccat gacaccgact
      121 tcgatcaacg ggaagtcacg cttcggccag actttcgtca gatcgaacgg gttgatcgga
      181 tagctacctg cttccttctc cggcatgacc tgcacgtaca gggtccagcg cggaaactcc
      241 tggcgttcaa tcgcttcgta caggtcgcga tgatgcgtct cgcgatcccg gccgacgagc
      301 gcttccgctt ctgcatccgt caggttttcg atgccctgtt gcgagcggaa atggaacttt
      361 acccagaagc gttcgtttgc ggcattaata aagctgaacg tgtggctgcc gaatccgtgc
      421 atgtggcgga acgtcttcgg aataccgcga tcgctcatga cgatcgtgac ttgatgcagc
      481 gcttccggca gttgcgtcca gaagtcccag ttgctctccg cgctgcgcaa gccggtgcgc
      541 ggatcgcgct tgatcgcgtg gttcaggtcc gggaacttga gcggatcacg caggaagaac
      601 accggcgtgt tgttgcccac cagatcccag ttgccttcct cggtatagaa cttcaacgcg
      661 aagccgcgga tatcccgctc ggcgtccgcc gcgccgcgct cacccgcgac ggtcgagaag
      721 cgcgcgaaca tctcggtctt tttgccgatc tccgagaaga tcttcgcgcg ggtgtactgc
      781 gtgatgtcgt gggtcacggt gaaggtgccg aatgcaccag aacctttcgc gtgcatgcga
      841 cgttcaggaa tcacttcacg gtcgaagtgg gccagtttct ccagaaacca tacgtcttcg
      901 agcaatgccg gtccgcgtgg accggcggtc ttgatgttct ggttgtcgac gacaggtgcg
      961 ccgaaaacgg tagtcagctt tttcatgaaa gatcctctat ctacgcaatt gggcagcggc
     1021 agccgttcag ttgcccttag ccgccgaatc agtgccgcag ccgcagccct gctggtgagc
     1081 gtgaccggcc ttgctgtcgt cccacgcgga acccaggatg atctggcaga aattcaggcg
     1141 cttgtacgac ggatcgcgca gttcgagcac gtctgccttg acgttgccga aggtcgtctc
     1201 gggcttcctg atcgttcctt gcgcgaaggc atcgatgatg ccgttcttga acgtctcctc
     1261 acgcggatgc gccgccacta cctgaatccg ctgttcgtcg ctgaactcgt ggtaggccat
     1321 gccgagcacg tccatctcga ccccggcggt cacgagcgcg acgaccggct tcagatgttc
     1381 gggtacgccc ggtgtggtgt gcagcgcaat cgacaaccac acctgctcga tgtcgtagtc
     1441 gttcatgccg tgctgcgtca tgaaatcgcg cgctgcattg gcgccatcca cttcgaaacg
     1501 gtagccttcg ctgctatacg cgggcatcag gcccatgtcg tggaacatgg cgccgaggta
     1561 cagcagttcc gcgtcgtact taagaccctt gcgttcgccg ctgagggcgc cgaac
//
LOCUS       DATASH010000569         2319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2862340,
            whole genome shotgun sequence.
ACCESSION   DATASH010000569 DATASH010000000
VERSION     DATASH010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2862340"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..936
                     /gene="pcaD"
                     /locus_tag="VHV99_15520"
     CDS             145..936
                     /gene="pcaD"
                     /locus_tag="VHV99_15520"
                     /EC_number="3.1.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047570 - 3-oxoadipate enol-lactonase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipate enol-lactonase"
                     /protein_id="HEX3636867.1"
                     /translation="MPYAAVNGIELHYRIDGDRHGNAPWIVLSNSLGSDLSMWTPQVA
                     ALSKHFRVLRYDTRGHGHSEAPKGPYTIEQLTGDVLGLMDTLKIARANFCGLSMGGLT
                     GVALAARHGNRLERVVLSNTAARIGSPEVWVPRAARARTEGMLALADAVLPRWFTADY
                     IEREPVVLAMIRDVFVHTDKEGYASNCDAIDATDLRPEAPGIKVPTLVISGTHDLAAT
                     PAQGRELAQAIPGARYVELDASHISNIEKVDAFTKTVLDFLTEQK"
     gene            933..1334
                     /gene="pcaC"
                     /locus_tag="VHV99_15525"
     CDS             933..1334
                     /gene="pcaC"
                     /locus_tag="VHV99_15525"
                     /EC_number="4.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047575 - 4-carboxymuconolactone
                     decarboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxymuconolactone decarboxylase"
                     /protein_id="HEX3636868.1"
                     /translation="MNDEDRYEAGLEVRRAVLGSAHVDRSLANRTELTEEFQNFITRY
                     AWGEIWTREGLPRHTRSLLTIAMMVALNRSEELALHLRAAKNNGVTREQIKEVLLQSA
                     IYCGVPAANSAFHLADKLFREDDAAAAAPKP"
     gene            complement(1388..2089)
                     /locus_tag="VHV99_15530"
     CDS             complement(1388..2089)
                     /locus_tag="VHV99_15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX3636869.1"
                     /translation="MSPTAARKPGAIGIRAKQAQDTRAKILKAAIKVFAKQGYASGRV
                     ESISKAARSHDRMIYYYFGSKEQLFVEVLETIYTQFNEAESRLDLDLGDPVHGLEQMV
                     EFVWQYYLDHPEFVTLLSSENLHQGKHAKKSSKLKEISGYAISVVQKLLDAGQAQNVF
                     RTDVKARDVYLMIASLGYFYNANQYTLGAFLGESLMDKAALAHWREVIKDTVLRAVRL
                     QLPAETMVVHESKTA"
BASE COUNT          467 a          709 c          687 g          456 t
ORIGIN      
        1 cgtgaccgaa catctttcgc ccgagcgcct gaagcaactg ctcgaccccg ctcaatacgt
       61 cggccaggcg cacgcttatg tggacgccgc gctggcactt catcacgcgc gcgcgagccg
      121 cgctacttcc aaggagtaat ccgcatgccc tacgccgcag tcaacggtat cgagctgcac
      181 tatcgaatcg acggagaccg tcacggcaac gcgccgtgga tcgtgttgtc gaattcgctc
      241 ggcagcgatc tgtcgatgtg gacgccgcaa gtcgcggcgc tgtcgaaaca ctttcgtgtg
      301 ctgcgctacg acacgcgcgg tcatggtcat tcggaagcgc cgaaaggtcc ctacacgatc
      361 gaacagttga ccggcgatgt gctcggcctg atggatacgc ttaagatcgc acgcgcgaat
      421 ttctgcgggc tgtcgatggg cggcctgacc ggcgttgcac tggccgcgcg tcacgggaat
      481 cgcctcgagc gcgtggtgct gtccaatacg gcggcgcgta tcggttcgcc ggaagtgtgg
      541 gtgccgcgcg ccgcgcgggc acgcacggag ggcatgctcg cgctggctga tgctgtgctg
      601 ccgcgctggt ttaccgccga ctacatcgaa cgtgaaccgg ttgtgcttgc catgattcgc
      661 gatgtgttcg tgcacaccga caaggaaggc tacgcatcga actgcgatgc catcgacgcc
      721 accgatctgc gccccgaagc gcccggtatc aaggtgccaa cgctcgtgat cagcggcacg
      781 cacgatctgg ccgcgacgcc ggcacaaggc cgcgaactgg cgcaggcgat tccgggcgcg
      841 cgttacgtcg aactggacgc ctcgcatatt tccaacattg aaaaagtcga cgctttcacg
      901 aagaccgtgc tcgacttcct gacggagcag aaatgaacga cgaagaccgc tacgaagctg
      961 gactcgaggt acgccgcgcg gtgctgggca gcgcgcacgt tgaccgctcg ctcgccaatc
     1021 gcactgaatt gacggaggaa ttccagaact tcatcactcg ctatgcatgg ggcgaaatct
     1081 ggacgcgcga gggattgccg cgccacacgc gcagcttatt gaccatcgcg atgatggtcg
     1141 ccttgaaccg gagcgaagaa ctcgcattgc atttgcgcgc ggcgaagaac aacggcgtga
     1201 cgcgcgagca gatcaaggaa gtgctgctgc aaagcgcgat ctattgcggc gtgccggcgg
     1261 ccaattcggc gttccatctc gccgataaac tgttccgcga agacgacgcg gcagcggcag
     1321 cgccaaagcc gtaacggcgc agttgcagag atgcagagcc tgaagcttac gtcagacgaa
     1381 acgcgcttca ggcagttttg ctctcatgca ccaccatggt ttcagccggc aattgcaagc
     1441 gcaccgcgcg caacaccgta tccttgatga cctcgcgcca atgcgcgagc gccgccttgt
     1501 ccatcagcga ttcaccgaga aacgcaccca gcgtgtactg attggcgtta tagaaataac
     1561 cgagcgacgc aatcatcagg tacacatcac gcgccttgac gtcggtacga aaaacgttct
     1621 gcgcttgacc cgcgtccagt aacttctgca cgaccgaaat cgcatagccc gaaatctcct
     1681 tcagctttga cgatttcttc gcatgcttgc cctgatgcag attctcgctc gacagcaggg
     1741 tgacgaattc cggatgatcg agatagtact gccagacgaa ttcgaccatc tgttcgaggc
     1801 catgcacggg atcgcccaga tcgagatcga gcctgctttc ggcttcgttg aactgcgtgt
     1861 agatcgtttc caggacttcg acaaacaact gttccttgct gccgaagtag tagtagatca
     1921 tgcggtcgtg cgaacgcgcg gccttcgaaa tgctttcgac ccggccgctt gcatagcctt
     1981 gttttgcaaa gaccttgatt gccgctttga gaatcttggc gcgcgtgtct tgcgcctgtt
     2041 tcgctcggat gccgatagcg cccggcttgc gggcggcagt gggactcatg gcgggctcgg
     2101 aactctttgt tagcgtcagg ttgaacgaca tgcgccgcag ccgcttacga gcactcagct
     2161 acgcttcggc atggcgcgcc ttcatcatac agcccatctc atgcgcgacg gaatgccctg
     2221 tacgatcatg cgcttcggcg tagattgaag cgtcattcgc gcgccgatgc cgcgccgcag
     2281 cattcaggcg tgcgccggga aactcgttgc gcagtctat
//
LOCUS       DATASH010000570         3196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6027919,
            whole genome shotgun sequence.
ACCESSION   DATASH010000570 DATASH010000000
VERSION     DATASH010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6027919"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1283)
                     /gene="rseP"
                     /locus_tag="VHV99_15535"
     CDS             complement(<1..1283)
                     /gene="rseP"
                     /locus_tag="VHV99_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018417653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEX3636870.1"
                     /translation="MNLLIELLAFAVAIGVLVVVHEYGHYSVARLCGVKVLRFSIGFG
                     KPLFQWVSPKTGTEWTIAALPLGGYVKMLDERETGGAPIPAEALPHAFNRQSVWRRFA
                     IVAAGPVANFLLAIVLFALVFATGVTEPAAVVAAPAPNTPAALAGFDGGETIVAVRAE
                     NAGEAERVRSWSDLRWKLLGAAFDHKRVVLSAKDAHGTSDFQLDLRGITEKDVDDDFM
                     SHLGFEPGGGKLTVAGVQPDSAAQKAGLAVGDRLRAINGIPTDNATAFIAYVKSHAGT
                     PMTLQVERGGQAAGKLEDVRIVPQSQRDDATGQQIGRIGAELATQVPSIDVHYGPVES
                     LQLGARRTWDLAVYSVRMFGRMIVGEASLKNLSGPVAIADYAGKSARLGPSAFLSFLA
                     LVSISLGVLNLLPIPVLDGGHLLYYLVEAVTGKVVS"
     gene            complement(1291..2496)
                     /locus_tag="VHV99_15540"
     CDS             complement(1291..2496)
                     /locus_tag="VHV99_15540"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEX3636871.1"
                     /translation="MQKRLTLLGSTGSIGDSTLDVVARHPERFSVYALTAHRNGDKLV
                     EQCLRFQPEVAAVGDADTAARVAAKLREAGCKTEVTYGPQALVDVSQSDGCDTVVAAI
                     VGAAGLAPGLAAARAGKRILLANKEALVMSGAIFMDAVRDNGAVLLPVDSEHNAIFQC
                     LPREAALHGGVSKIILTASGGPFRTREPATLVDVTPDEACKHPNWVMGRKISVDSATM
                     MNKGLEVIEAHWLFNLPGERIEVLIHPQSVIHSMVSYADGSVLAQLGNPDMRTPIAHA
                     LAFPDRVDSGVAQLDLLQVASLSFEKPDYARFPCLALAVKALAEGGLASAALNAANEI
                     AVEAFLSRQIGFMAIARVVDAVLNALPNRSAHALEDVIEADAAARRAAADFIACLPDG
                     ARRTERAVQ"
     gene            complement(2507..>3196)
                     /locus_tag="VHV99_15545"
     CDS             complement(2507..>3196)
                     /locus_tag="VHV99_15545"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004521189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX3636872.1"
                     /translation="RLLKLGGAGPVIYALVAAVALVASTRLGAGVEEPRPLFQAAAIF
                     WVIAGPYVLLRKPTLAQGAWRAFLFLAGIVVFVACWHALVAARMKGVPFVLSLLLLVW
                     LADIGAYFSGKAFGKHKLAPAISPGKTWEGAIGGWLVVMIVAAAAVFWHAFEPTLYSA
                     LLAQLGAVRTLLALTLLVAFSVVGDLFESMMKRQAGVKDSSGLLPGHGGVLDRIDALL
                     PVLPLAMLLLG"
BASE COUNT          638 a         1075 c          970 g          513 t
ORIGIN      
        1 gagacaactt tgccggttac agcttcaacc aaataatata acagatgacc cccgtccaat
       61 accggaattg gtaacaggtt cagtacgccg aggctaatac tgacaagggc caggaacgac
      121 aggaatgcag aaggacctag acgtgcgctc tttcctgcgt aatcggcgat cgccacgggg
      181 ccagaaagat tcttgagcga agcctcgccg acgatcattc gtccgaacat gcgcaccgaa
      241 taaacagcaa gatcccatgt gcggcgcgcg cccagttgca ggctttcgac cggaccgtaa
      301 tgcacgtcga tcgacggcac ctgcgtggcg agttccgcgc cgatacggcc gatctgctgc
      361 cccgtcgcgt catcacgctg cgactgaggg acgatgcgaa cgtcttccag cttgcccgcc
      421 gcttgcccac cacgctctac ctgcagcgtc atgggcgtgc cggcgtgcga ttttacatag
      481 gcgatgaagg ccgtggcatt gtcagttggg ataccgttga tcgcgcgcag acggtcaccc
      541 actgcaagac ctgccttttg cgccgcgcta tccggttgca cgcccgcgac cgtcagcttg
      601 ccaccgcccg gctcgaaacc gagatgcgac atgaagtcgt cgtcgacgtc tttctcggtg
      661 atgccgcgca gatcaagttg gaaatccgag gtgccgtggg catctttcgc actcagtacg
      721 acgcgcttgt gatcgaacgc cgcacccagc aatttccagc gcaaatcgga ccacgagcgc
      781 acccgctcgg cttcgccggc attctccgca cgcaccgcga caatggtttc gccgccgtcg
      841 aaaccagcca acgcggccgg cgtattggga gcgggtgctg caaccacggc cgccggctcc
      901 gtcacgccgg tggcgaacac gagggcgaac agcacgatgg caagcaggaa gttcgcgacc
      961 gggcctgccg cgacaatcgc gaaacggcgc cacaccgact gccggttgaa cgcgtgcggc
     1021 aaggcttcgg cgggaatcgg cgcgccgccg gtctcgcgct cgtcgagcat cttcacgtag
     1081 ccgcccagcg gcagcgcggc aatcgtccat tccgtgccgg tcttcgggct cacccattgg
     1141 aacagcggct tgccgaagcc gatcgaaaaa cgcaacacct tgacgccgca caaacgcgcc
     1201 acgctgtaat gcccgtactc gtggacgacc acgagtacgc caatcgcgac cgcgaaggca
     1261 agcagttcga tcagcagatt cataggcgcc tcactggacg gcgcgttccg tgcgacgggc
     1321 gccgtccggc agacacgcga tgaagtcggc agcggcgcgg cgcgcggccg catccgcttc
     1381 gatcacgtct tccagtgcat gcgcgctacg gttcggcaac gcgttgagca ccgcgtcgac
     1441 cacccgggcg atcgccatga aaccgatctg ccgcgacagg aacgcttcaa cggcgatttc
     1501 gttcgcggcg ttcagggccg cgctcgcgag gcctccctcg gccagtgcct tcaccgcaag
     1561 cgcgagacac gggaaacgcg cgtaatccgg cttttcgaac gaaagcgatg cgacctgcag
     1621 caggtcgagt tgtgccacac ccgaatcgac ccgatccgga aacgccagcg catgagcaat
     1681 cggcgtgcgc atgtcggggt tgcccagttg cgcgagcacc gaaccgtcgg cgtacgacac
     1741 catcgaatga atcacgcttt gcgggtggat cagcacctcg atgcgctcgc ccggcagatt
     1801 gaagagccag tgcgcctcga tcacctcgag gcccttgttc atcatcgtgg ccgagtcgac
     1861 cgaaatctta cggcccatca cccagttcgg atgcttgcag gcttcgtcag gcgtgacgtc
     1921 gaccagcgtg gccggttcgc gcgtgcggaa cggaccaccc gacgcggtca ggatgatctt
     1981 cgagacgccg ccgtgcagtg cggcttcgcg cggcagacat tggaaaattg cgttatgttc
     2041 gctgtcgacc ggcagcagca cggcaccgtt gtcgcgcacc gcgtccatga agatcgcgcc
     2101 ggacatcacc agcgcttcct tgttggcgag cagaatgcgc ttgccggcgc gcgccgccgc
     2161 gagacccggc gcgagacccg cagcgccgac gatcgccgcc accacggtat cgcagccgtc
     2221 gctctgcgac acgtcgacga gcgcttgtgg cccgtacgtg acttcggtct tgcacccggc
     2281 ttcgcgcagt ttcgccgcga cgcgcgcggc cgtatcggcg tcgccgactg ccgcgacttc
     2341 gggctgaaag cgcaggcatt gctcgacgag cttgtcgccg ttgcgatgcg ccgtgagcgc
     2401 ataaaccgaa aagcgctcgg gatgacgcgc gacgacgtcg agcgtgctgt ctccaatcga
     2461 gcccgtggaa ccgagcaatg tcagacgttt ttgcatatct ctttctctag ccgagcagca
     2521 gcatggcaag cggaagcacc ggcaataatg catcgatgcg gtcgagcacg ccaccgtgcc
     2581 caggcaacag gccgcttgaa tctttcactc cggcctggcg tttcatcatt gattcgaaca
     2641 ggtcgcccac cacgctgaat gccaccagca aggtcagcgc gagcagcgta cgtacggcgc
     2701 ccagttgcgc cagcagcgca gaatacaggg ttggctcgaa cgcatgccaa aagaccgccg
     2761 ccgccgcgac gatcatgact accagccagc cgccgatcgc gccctcccaa gtcttacccg
     2821 ggctgatggc aggcgccagc ttgtgtttgc cgaacgcttt tccggagaag tatgcgccga
     2881 tatcggccag ccataccaat agcagcagcg acagcacgaa cggcacgcct ttcatgcgtg
     2941 cggcgacgag agcatgccag cacgcgacga ataccacaat gccggcaagg aatagaaaag
     3001 cgcgccaggc gccctgcgcg agggtcggct tgcgcaggag cacatacggc cccgcgatca
     3061 cccagaaaat agcggccgcc tgaaagagcg gccggggctc ctcgacaccg gcgccgagcc
     3121 gcgtgctcgc gaccagcgca accgcggcca ccagcgcgta aatgaccggg cccgcgccac
     3181 cgagcttcag caggcg
//
LOCUS       DATASH010000571         2152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5634609,
            whole genome shotgun sequence.
ACCESSION   DATASH010000571 DATASH010000000
VERSION     DATASH010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5634609"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="VHV99_15550"
     CDS             <1..741
                     /locus_tag="VHV99_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014897649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEX3636873.1"
                     /translation="QERLEREFDMDLITTAPTVVYEVLQRDGTIIMVENPAKMPEPSK
                     IEEVREPIVTVNLYMPQDYVGSVITLCTQKRGNQINMQYHGRQVQLTYEIPMGEVVLD
                     FFDRLKSISRGYASMDYEFKEYRAADVVKVDMLINGDKVDALSVIVHRSQSQYRGREV
                     ASKMRELIPRQMYDVAIQATIGANIIARENIKALRKNVLAKCYGGDISRKKKLLEKRK
                     AGKKRMKQVGSVEIPQEAFLAILRVEDK"
     gene            760..1653
                     /gene="lepB"
                     /locus_tag="VHV99_15555"
     CDS             760..1653
                     /gene="lepB"
                     /locus_tag="VHV99_15555"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX3636874.1"
                     /translation="MNFALILFVLVILTGIAWVADKLVFMPQRRRAAEAAVAEFDRQQ
                     ARVGERFADENAAQTRARLRDDKLRQPWWLEYSASFFTVILVVFVVRSFVVEPFKIPS
                     GSMVPTLLVGDFILVNKFEYGIRLPIINTKITEGRPLERGDVVVFRYPKDESVDYIKR
                     VIGLPGDTVAYQDKQLTINGKPVPETPLPDYLDEERLGYAKQFEEDLGGRKNAILNNP
                     AVPPFIVGAEDYPYRDNCTYNARGVICKVPPGNYFMMGDNRDNSADSRYWGFAPDQNV
                     VGRAFFIWMNFSDLKRIGSFH"
     gene            1829..>2152
                     /locus_tag="VHV99_15560"
     CDS             1829..>2152
                     /locus_tag="VHV99_15560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HEX3636875.1"
                     /translation="MPLSPLESRLRYEFRNAELLRQALTHRSHSSTHNERLEFLGDSV
                     LNCAVAALLFQRFGKLDEGDLSRVRANLVKQQSLYEIAQALNISEGLRLGEGELRSGG
                     FRRPSI"
BASE COUNT          455 a          617 c          644 g          436 t
ORIGIN      
        1 caggagcgtc tcgagcgcga gttcgacatg gatctgatca cgacggcgcc gaccgtggtg
       61 tacgaagtcc tgcagcgcga cggtacgatc atcatggtcg aaaatccggc caagatgccg
      121 gagccgtcga agatcgagga agtgcgcgag ccgatcgtca ccgtgaatct gtacatgccg
      181 caagactatg tcggctcggt gatcacgctg tgcacgcaaa agcgcggcaa ccagatcaac
      241 atgcagtacc acggccgcca ggtccagttg acgtacgaaa tcccgatggg cgaggtcgtg
      301 ctcgatttct tcgatcgtct gaagtcgatc tcgcgcggct atgcgtcgat ggattacgag
      361 ttcaaggaat atcgcgcggc agacgtcgtg aaggtcgaca tgctgatcaa cggcgacaag
      421 gtggatgcgt tgtcggtaat cgtgcaccgt tcgcagagcc agtatcgcgg ccgcgaagtg
      481 gcgtcgaaaa tgcgcgagct gattccgcgg caaatgtacg acgtggcgat tcaggcgacc
      541 atcggcgcga acattattgc gcgcgaaaac attaaagcgt tgcgtaagaa cgtgctggca
      601 aaatgctacg gcggcgatat tagccgcaag aagaaactgc tcgaaaaacg aaaggcaggc
      661 aagaagcgaa tgaagcaggt cgggtccgtc gagattccgc aagaggcttt cctcgcgatc
      721 ctgcgtgtcg aagacaaata agacgaacca cggatcctta tgaattttgc gctgattctt
      781 tttgtgctcg tcattttgac gggcattgca tgggttgcag ataaactggt tttcatgccg
      841 caacggcgcc gcgcggcgga agccgcggtc gctgagttcg accgccagca ggcacgcgtc
      901 ggcgagcgtt tcgccgacga aaacgcggcg caaacacgcg cccggctgcg tgacgacaag
      961 ctgcgtcaac cgtggtggct cgagtattcg gcgagctttt tcacggtgat tctggtggtg
     1021 ttcgtggtgc gctcgttcgt ggtcgaaccg ttcaagattc cgtcgggctc gatggtgccg
     1081 actctgctgg tgggcgactt catcctcgtc aacaaattcg agtacggcat tcgcctcccg
     1141 atcatcaaca cgaagatcac cgaaggccgt ccgctcgagc gcggcgacgt ggtggtgttc
     1201 cgttacccga aagacgaatc ggtggattac atcaagcgcg tgatcggctt gccgggcgac
     1261 accgttgcct atcaggacaa gcaactgacg atcaacggca agccggtgcc ggaaacgccg
     1321 ctgcctgact acctcgacga agaacgcctc ggctacgcga agcaattcga agaagatctc
     1381 ggcggccgca agaacgcgat tctgaacaac ccggccgtgc cgccgttcat cgtcggcgcc
     1441 gaagattatc cgtatcgcga taactgcacg tacaacgcac gcggcgtgat ttgcaaagtg
     1501 ccgccgggca attacttcat gatgggcgac aaccgcgata acagcgccga tagccgctac
     1561 tggggttttg cgccggatca gaatgtggtg ggtcgcgcgt ttttcatctg gatgaacttc
     1621 agcgatctga agcgcatcgg ctcattccac tgagcacgca tgtcttgatt gcacgacacg
     1681 ccgcgcaggt ggcctccact tcggtcacat cagtcgcggc gtgttagcac gctactttaa
     1741 gaatccgcgg taacgtcgct tcaccacgct ttttccgctg gccgtcccgc gttttcgccg
     1801 gggcaggcgc ccgcgttata ctctgcccat gcccctatct ccgttggaaa gccgtctgcg
     1861 ctacgaattt cgcaatgcgg aattgttgcg ccaggcttta acgcaccgca gtcatagctc
     1921 cacgcacaac gaacggctcg agttcctcgg cgactccgtt ctgaattgcg cggtggctgc
     1981 gcttttgttc caacgtttcg gcaaactgga cgaaggcgac ctgtcgcgcg tccgcgccaa
     2041 tctggtcaaa cagcagtcgc tttacgagat cgctcaggcc ctgaatatct ccgaagggct
     2101 gcgtctcggt gagggcgaac tgcgcagcgg cggcttccgc cgcccgtcga tc
//
LOCUS       DATASH010000572         2017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1875494,
            whole genome shotgun sequence.
ACCESSION   DATASH010000572 DATASH010000000
VERSION     DATASH010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1875494"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VHV99_15565"
     CDS             complement(<1..249)
                     /locus_tag="VHV99_15565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3636876.1"
                     /translation="MIRFNQFSLARGTKPLFENTTFTLNPGEKAGLVGANGAGKSTLF
                     AVLLGELHADGGDFSIPPNWQIAHVAQETPAADKTALAY"
     gene            385..864
                     /locus_tag="VHV99_15570"
     CDS             385..864
                     /locus_tag="VHV99_15570"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEX3636877.1"
                     /translation="MTSIYSFSARTLGGEEVSLERYQGKVLLIVNTASECGFTPQYAG
                     LQKLYDSYAARGLAVLGFPCNQFGKQEPGDAAQIGSFCEKNYGVTFPMFDKIDVNGAN
                     AHPLFRYMTGEAPGLLGLEGIKWNFTKFLVGRDGNVVKRYAPLTKPEAITEDIEKLL"
     gene            complement(1066..>2017)
                     /gene="cls"
                     /locus_tag="VHV99_15575"
     CDS             complement(1066..>2017)
                     /gene="cls"
                     /locus_tag="VHV99_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEX3636878.1"
                     /translation="ALIAKAQQGVRVYFLYDSIGSFDLPHRYVAALRAAGVETHPFAT
                     NRRFVNRLQLNFRNHRKIVSVDGERAFVGGHNVGVEYLGGKPPLSPWRDTHIEVRGPA
                     VASIQFVFTEDWHWATQQLPEFDMPAFQPTEGGMHCLVVPSGPADRQETCSLFFVEAI
                     NAARERIWITTPYLVPDEAVFAALRLAALRGVDVRILIPCRRDHRVVFAASKLYAYDS
                     LRAGIRIFRYQPGFLHQKVVLIDGVAAAIGSANLDNRSFRLNFEIMVLTVDRGFAKDV
                     EAMLLKDFADSLEIDRNEYRQASALRRVLMHVARLFSPIL"
BASE COUNT          397 a          591 c          674 g          355 t
ORIGIN      
        1 ataggcgaga gcggttttgt ccgcggcggg cgtttcctgc gcgacgtggg cgatctgcca
       61 gttcggcggg atagagaaat cgccgccgtc ggcgtgcagt tcgccgagca gcacggcaaa
      121 tagcgtcgac ttgcccgcgc cgttggcccc caccaggccg gccttctcgc ctgggttgag
      181 ggtgaacgtg gtgttttcaa agagcggctt ggtgccgcgc gcgaggctga actggttaaa
      241 gcggatcacg aagaggccgg ctggagaaaa acgctatttt agaccgccgg ggctgacacc
      301 gggcactcag ccaggatcgg ccattcggcc gactgtaccg gcgaagcttt tggcatagac
      361 tgacggcttt cacggggaga gcacatgaca tcaatttatt cgttttccgc acgtacgctc
      421 ggtggcgagg aagtgagcct cgagcgctat caaggcaagg ttctgttgat cgtcaacacg
      481 gctagcgaat gcggcttcac gccgcagtac gcgggtctgc agaagcttta cgacagctac
      541 gcggcacgtg gactcgcggt gctcggtttt ccgtgcaacc agttcggtaa gcaggaaccg
      601 ggtgacgccg cgcagatcgg cagcttctgc gaaaagaatt acggcgtgac cttcccgatg
      661 ttcgacaaga tcgatgtgaa tggcgccaat gcgcatccgc tgttccgcta tatgaccggc
      721 gaggcgccgg gcttgctagg gctcgagggg atcaaatgga acttcaccaa gttcctggtc
      781 ggccgcgacg gcaatgtggt gaagcgctat gcaccgctga ccaagcccga ggcgattacc
      841 gaggacatcg aaaagctgct gtaacggggc gcggagcagg gagttcggct cgggcggggg
      901 gctggaatca gtgaacggtc aggagccagc gtccaaaact ggatccgaac tgatgccgat
      961 gccgtgccga cgccgatgca gatgcagatg ccgaagccga agccgaagct gtgccgatgc
     1021 cgatgctgtg ccggtgccgg cttcagcggc gcagcagcgg tgcggctaca gaatcggcga
     1081 gaacaaccgc gccacgtgca tcagcacgcg ccgcagcgcg cttgcctgac ggtactcgtt
     1141 gcggtcgatt tcgagcgaat ccgcgaagtc cttgagcagc atcgcttcga catccttggc
     1201 aaagccacgg tccacggtca gcaccatgat ctcgaagttc agccggaacg agcggttgtc
     1261 gagattggcg ctgccgattg ccgctgccac gccgtcgatc agcacgacct tctggtgcag
     1321 gaagcccggc tggtagcgga agatccggat gcccgcacgc agcgagtcgt acgcatacag
     1381 ttttgaggcg gcgaacacca cgcggtgatc gcgccggcat ggaatcagaa tgcgcacatc
     1441 cacgccgcgc agcgccgcca gccgcaatgc ggcgaacacg gcttcgtcgg gcacgaggta
     1501 aggcgtggtg atccagatgc gctcgcgtgc cgcgttgatc gcctcgacga agaaaagcga
     1561 gcaggtctcc tgcctgtcgg ccggaccgct cggcacgacg aggcagtgca tgccaccttc
     1621 ggttggctgg aaagcgggca tgtcgaactc gggcagttgc tgggtcgccc agtgccagtc
     1681 ttcggtgaac acgaactgga tgctggccac ggccgggcca cgcacttcga tatgcgtatc
     1741 gcgccatggc gacaaaggtg gtttgccgcc gagatattcg acaccgacgt tgtgcccgcc
     1801 gacaaacgcg cgttcaccgt ccaccgagac gatcttgcgg tggttgcgga agttcaactg
     1861 caggcggttg acaaagcggc gattggtggc gaacgggtga gtctccacgc cggccgcccg
     1921 cagcgccgcc acgtaacggt gtggcagatc gaaactgccg atgctgtcgt acaggaagta
     1981 gacgcgcacg ccttgctgcg ctttcgcgat cagcgcg
//
LOCUS       DATASH010000573         1506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3455729,
            whole genome shotgun sequence.
ACCESSION   DATASH010000573 DATASH010000000
VERSION     DATASH010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3455729"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /gene="rpsA"
                     /locus_tag="VHV99_15580"
     CDS             complement(<1..200)
                     /gene="rpsA"
                     /locus_tag="VHV99_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589239.1"
                     /note="in Escherichia coli this protein is involved in
                     binding to the leader sequence of mRNAs and is itself
                     bound to the 30S subunit; autoregulates expression via a
                     C-terminal domain; in most gram negative organisms this
                     protein is composed of 6 repeats of the S1 domain while in
                     gram positive there are 4 repeats; the S1 nucleic
                     acid-binding domain is found associated with other
                     proteins; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEX3636879.1"
                     /translation="MQILIFMSDLQTSNPNTESFAALFEESLTKQDMRAGEVISAEVV
                     RVDHNFVVVNAGLKSEAYIPLE"
     gene            complement(368..1054)
                     /gene="cmk"
                     /locus_tag="VHV99_15585"
     CDS             complement(368..1054)
                     /gene="cmk"
                     /locus_tag="VHV99_15585"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012400154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEX3636880.1"
                     /translation="MKPIRPFHQTPVITIDGPSASGKGTVAALVAASLGFHLLDSGAL
                     YRLAALASMRYNIVADDVDALVKLIDDLHITFREGLAQLDGADVSADIRAEEVGSRAS
                     AIAVHAPVRTALVARQRAFRKEPGLVADGRDMGTVIFQDAMLKVFMTASVEARAVRRH
                     KQLIQKGFSANIDDLLRDLRERDERDSQRAAAPLKPAADAKLLDTSALSVDQAVEQVV
                     QWYEAWVPHA"
     gene            complement(1089..>1506)
                     /locus_tag="VHV99_15590"
     CDS             complement(1089..>1506)
                     /locus_tag="VHV99_15590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018435646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX3636881.1"
                     /translation="EVRGVGNDHGKLDPIDMDFNLIPDAAMTIAVAALFADGTTTLRN
                     IASWRVKETDRIAAMATELRKVGAKVEEGEDYLVVTPPEKLIPNAAIDTYDDHRMAMC
                     FSLVSLGGVPIRINDPKCVGKTFPDYFERFTALAQP"
BASE COUNT          295 a          450 c          466 g          295 t
ORIGIN      
        1 tcttcgagcg ggatgtaggc ttcggacttg agaccagcgt tgacgaccac gaagttgtgg
       61 tcaacacgca cgacttcggc ggagatcacc tcgccagcgc gcatgtcttg cttggtcagc
      121 gactcttcga acagagccgc aaaagattcg gtattcgggt tggaagtttg caggtcggac
      181 ataaaaatca aaatttgcat ggttctcgcg gtgcccggct ggccgggacg gtcagcaaca
      241 acggcaagaa tgcgaatgcc acgcggggtt aaggggttaa tacacgctcc acgacacacg
      301 tggagcacct actgacaacg gtacttcttc tactgcgccc gctgctcata cagccataag
      361 gcaaccgtca agcgtgcggg acccatgctt cataccactg cacaacctgt tcgaccgcct
      421 ggtcgacgga caatgcggag gtatccagca gctttgcatc cgccgcgggc ttgagcggcg
      481 cggccgcgcg ctgactatca cgctcgtcgc gctcacgcaa atcccggagc aagtcatcta
      541 tattagcaga aaatcctttt tggatcaatt gcttatgccg gcgcaccgcg cgggcctcga
      601 cgctggcggt catgaacact ttcagcatgg catcctggaa gatcaccgtg cccatgtcac
      661 gaccgtcggc gaccagcccc ggttccttac ggaaagcccg ttgacgggcc accagcgcgg
      721 tccgcacggg cgcgtgcacg gcgatcgccg aagcgcggct gccgacttcc tccgcgcgaa
      781 tgtcggcgga aacgtcggca ccgtcgagtt gtgccagccc ttcgcgaaag gtgatgtgca
      841 gatcgtcgat cagtttgaca agtgcgtcga cgtcgtcggc cacaatgtta tagcgcatgc
      901 tggccagcgc ggccaaccgg taaagtgcgc cgctatccag caaatggaag ccgaggctcg
      961 cagcaaccag cgcggccacc gtgcctttgc cggaggcgct ggggccgtcg attgtaatga
     1021 cgggcgtctg gtgaaaggga cggatcggtt tcatcgaaaa agtcgcacgt cagtcgattc
     1081 ggagcgaatc agggttgagc cagcgcggtg aagcgctcga aataatcggg gaatgtcttg
     1141 ccgacgcact tcggatcgtt gatccggatc ggcacgccgc ctagacttac gagcgagaag
     1201 cacatcgcca tgcggtgatc gtcgtaagtg tcgatcgcgg cgttcgggat cagcttttcg
     1261 ggcggcgtga cgacgaggta gtcttcgccc tcctccacct tggcgccgac cttgcgcagt
     1321 tcggttgcca ttgcggcgat ccggtcggtt tctttcacac gccagctggc gatgttgcgc
     1381 agcgtggtcg tgccgtccgc gaacagcgcc gcgacggcga tcgtcatcgc ggcgtccgga
     1441 atcaggttga agtccatgtc gatcggatcg agcttgccgt ggtcgttgcc gacgccgcgc
     1501 acttcg
//
LOCUS       DATASH010000574         5027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_490140,
            whole genome shotgun sequence.
ACCESSION   DATASH010000574 DATASH010000000
VERSION     DATASH010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5027
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490140"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1224
                     /locus_tag="VHV99_15595"
     CDS             <1..1224
                     /locus_tag="VHV99_15595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HEX3636882.1"
                     /translation="RRSRGYAPAPLRLPRDLAAAPPVLAMGAQQKNTFCLLRDGEAVV
                     SHHIGELENGETYRDYSVSLARYQHLFEHEARLVAVDLHPEYLSRKLGIDIAQQRHLP
                     LAEIQHHHAHLAACLAENDVGLGTDVLGIVLDGLGMGLDNALWGGEFLLGGYVDFKRL
                     ASFTPVALPGGAYAIYEPWRNTYAHLSASLEWPRFAKQYAKLDLHHFLADRPLSLLDQ
                     MIDGGINSPVASSAGRLFDAVAAAFGVCRERVQYEGQAAIELEAMIDTRTLHDEDDAL
                     AYPFEIVEADTSLLINPKPMWITLLDDMLRATSVPVMAARFHKGLAIAIVQMTGRLLV
                     STQVRTVALSGGVMQNRVLFEQLTARLAACGFNVLAHRQVPSNDGGIALGQAAITAAR
                     ALASAFVEPRSTSCA"
     gene            1215..1544
                     /locus_tag="VHV99_15600"
     CDS             1215..1544
                     /locus_tag="VHV99_15600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HEX3636883.1"
                     /translation="MCLGIPGQIVEISDADNNLALVDVGGVQRIINIAFIVDDDRPPS
                     ACVGNWVLVHVGFAMSRLDEAEAVRTLALLRELGEAQAEMAAMMEGGGEPPLPDTFNH
                     HEGARHD"
     gene            1537..2238
                     /locus_tag="VHV99_15605"
     CDS             1537..2238
                     /locus_tag="VHV99_15605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX3636884.1"
                     /translation="MTEHRALHGLYPFLHGNRQDAAKLDAALLESVAQKAQASIDAKR
                     AFFADNTTAVVAVAHAIADVYRRDGRLFSMGNGGSSCDAAHIAVEFLHPVTAGRPALT
                     AVNLVADTAMMTAVGNDVGFAHIYVRQLVAQARRGDGLIGVSTSGNSDNLLAAFAKAR
                     EMGVVTIGLAGHDGGRMAAPGALDHCLVVPSDSIHRIQETHVAIYHILWDLVHTLLAD
                     ERGGLSVPDATGRVS"
     gene            2235..3416
                     /gene="hypD"
                     /locus_tag="VHV99_15610"
     CDS             2235..3416
                     /gene="hypD"
                     /locus_tag="VHV99_15610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HEX3636885.1"
                     /translation="MKYVDEFRDPEKARTLTHEIHRLVGQIVRDRGGLKRPLQIMEVC
                     GGHTHSIFRYGIHQMLPPEVEFVHGPGCPVCVLPMGRVDDCVALAERPEVIFTTFGDA
                     MRVPGSRKSLLKAKADGADVRMVYSPLDALAVARANPHREVIFFGLGFETTMPSTAMT
                     VLEAHAQRINNFSLFCNHITIIPTIKAILDSPDLHLDGFLGPGHVSMVIGTRPYRFIA
                     EHYRRPITVAGFEPLDVLQSVWMVLRQIAEGRCEVENQYGRIVPEDGNAQALSAIEQV
                     FELREFFEWRGLGSIDHSGVRIRERFAAYDAERKFPVANLKIADPKSCQCGEVLKGVI
                     RPQQCKVFGTACTPETPLGSLMVSSEGACAAHYRYARIDTSALYAHGAASDTLKQGVQ
                     A"
     gene            3413..4459
                     /gene="hypE"
                     /locus_tag="VHV99_15615"
     CDS             3413..4459
                     /gene="hypE"
                     /locus_tag="VHV99_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HEX3636886.1"
                     /translation="MNDRIGRLRDVTINLAHGGGGRAMRDLIEDVFVTAFDNPALAAL
                     EDQAVFPLADLARSGDRLAFTTDSYVVDPLFFPGGDIGTLAVSGTVNDLAMSGAKPLF
                     LSCGMVIEEGLAVDVLRRVAASMQRVAIEAGVSIVTGDTKVVERGCADKLFINTAGVG
                     VVPPGVNISARRAQPGDVVIVNGFLGDHGAAILVARKQLALQSDIESDCAPLASLVDA
                     MLGACKDIHCMRDATRGGVATVLNEFAASSGVAIRIRENALPIREEVKGACEILGLDP
                     LYLANEGKLVAIVPAHAASSVLAAMHAHPAGARAAVIGEVVAHPAGIVVLHTVFGGQR
                     IVDMLTGEQLPRIC"
     gene            4489..4818
                     /gene="hypA"
                     /locus_tag="VHV99_15620"
     CDS             4489..4818
                     /gene="hypA"
                     /locus_tag="VHV99_15620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HEX3636887.1"
                     /translation="MHELSIANSVVELCAERAMGARVLRVTLEIGRLSAVLPDAIRFC
                     FDVCAKDTVVEGAALEIVETPGRARCLDCGSNVEMTDLLQHCACGSVNLELLTGSELK
                     IKQMEVV"
     gene            4818..>5027
                     /locus_tag="VHV99_15625"
     CDS             4818..>5027
                     /locus_tag="VHV99_15625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636888.1"
                     /translation="MCTTCGCSKGAGSVITDPQTGERIESQVEDGSGAARKPGSWRQL
                     AGGAAGGSVAAHLHVRAHRHGHAAHI"
BASE COUNT          922 a         1457 c         1656 g          992 t
ORIGIN      
        1 cgccgctcgc gtggttatgc accggcgccg ctgcggttgc cgcgcgatct tgctgctgca
       61 cccccggtcc tcgcaatggg agcacagcag aaaaacacct tctgcctgct gcgcgatggc
      121 gaagcggtcg tgtcgcatca catcggcgaa ctcgagaacg gcgagacgta tcgcgactac
      181 agcgtgtcgc tcgcgcgtta tcaacatttg ttcgaacatg aagcgcgtct cgtcgcggtc
      241 gacctgcatc ccgagtatct gtcgcgcaag ctcggtatcg atatcgcaca gcagcggcat
      301 ttaccgcttg ccgagatcca gcatcatcac gcgcatctcg cggcatgtct tgcagagaac
      361 gatgtcggtt tgggcactga cgttcttggg attgtgcttg acgggcttgg catgggcttg
      421 gacaacgcgc tgtggggcgg cgaatttttg ctcggcggct acgtagattt caagcgtctc
      481 gcgagcttca caccggtggc gctgccggga ggcgcgtacg cgatttacga gccgtggcgc
      541 aacacgtacg cgcatctgtc cgcgagcctc gagtggccgc gtttcgcgaa gcaatacgcg
      601 aagctcgacc tccatcattt tctcgccgac cgtccattgt cgctgctcga tcagatgatc
      661 gacggtggga tcaatagtcc tgtcgcaagc tcggccgggc gtctgttcga tgcggtcgct
      721 gcggccttcg gcgtgtgccg cgaacgcgtt caatacgaag ggcaggcagc gattgaactc
      781 gaagcgatga tcgatacccg tacgctccac gatgaagacg acgcgttggc gtatccgttc
      841 gaaatcgtgg aagccgacac ttcgcttttg atcaacccga aacccatgtg gatcactctg
      901 ctcgacgaca tgctgcgcgc cacatccgtg ccggtcatgg ccgcacgttt tcacaagggt
      961 cttgcgatag cgatcgttca gatgaccggt cgtttgctcg tgtccaccca ggtccgtact
     1021 gtcgcactct ccggcggggt catgcaaaac cgcgtgctgt ttgaacaact caccgcacgc
     1081 cttgcggcat gcggttttaa tgtactggca catcgccagg taccgtccaa cgacggtggc
     1141 attgcgctgg ggcaagcggc aattaccgcc gcgcgtgccc ttgcatccgc atttgttgaa
     1201 ccacggagta cgtcatgtgc ttaggcatcc cgggacagat cgtcgagatc agcgacgcgg
     1261 acaataacct cgcattagtc gatgtaggcg gggtgcaacg gatcatcaac atcgcgttca
     1321 tcgtcgatga cgatcggccg ccttccgcat gcgtgggtaa ctgggtgctg gtgcatgtcg
     1381 gctttgcgat gagccgtctc gacgaagccg aagccgtgcg cacgcttgcc ctgttgcgtg
     1441 aacttgggga ggcgcaggcg gagatggccg cgatgatgga agggggcggc gagccaccac
     1501 ttcctgacac gttcaaccac cacgaaggag cacgccatga ctgaacatcg ggcgctacac
     1561 ggcctgtatc cgttccttca cggcaaccgt caggacgcgg caaaactcga cgccgcgctg
     1621 ctcgaatcgg tcgcgcagaa ggcgcaggca agcatcgacg cgaagcgcgc gttcttcgcg
     1681 gataacacca ccgccgtggt cgctgttgcg cacgcgatcg cggatgttta tcgccgtgac
     1741 ggcaggctct tttcaatggg caacggcggt tcgagttgcg acgccgcaca catagcggtg
     1801 gagttcctgc atccggtgac ggccgggcgt ccggcgctca ctgcagtcaa cctcgtcgct
     1861 gataccgcca tgatgacggc cgttggcaac gacgtcggct ttgcgcatat ctatgtgcgg
     1921 caactagtgg cgcaggcgcg ccgtggcgac ggcctgatcg gggtatcgac cagtggtaac
     1981 tcagacaacc tgctcgccgc attcgcgaag gcgcgggaga tgggcgtggt gaccatcggc
     2041 ttggccggac acgacggcgg caggatggcg gcgccgggtg cgctcgacca ttgcctcgtg
     2101 gtgccgagcg acagcattca tcggatccag gaaacccacg ttgcgattta tcacatccta
     2161 tgggacctgg tccatacgct gctcgccgat gagcgcggcg gcctgagtgt ccccgatgca
     2221 accgggcgcg tgtcatgaaa tacgtcgacg aattccgcga tccggagaaa gcccgcacgc
     2281 tcacgcacga gatccatcgt ctggtcgggc agatcgtgcg cgaccgtgga ggccttaaac
     2341 ggccgctgca gatcatggaa gtctgcggcg gtcatacgca ctccatcttc aggtacggga
     2401 ttcaccagat gctgccgccc gaggtcgaat tcgtccacgg ccccggttgc ccggtctgcg
     2461 tgttgccgat gggccgcgtg gatgactgcg tggcgctcgc cgagcggccc gaagtgatct
     2521 tcacgacctt cggcgacgcg atgcgtgttc ccggttcgcg caagagcctg ttgaaagcga
     2581 aggcggatgg agcagatgtg cgaatggtct attcgccgct cgacgcgctt gctgttgcgc
     2641 gtgcgaatcc gcatcgcgag gtgatcttct tcgggctggg ttttgaaacc accatgccga
     2701 gcacggcgat gaccgtgctg gaagctcacg ctcaacgcat caataacttc tcgctatttt
     2761 gcaaccacat cacgatcatt ccgacgatca aggcgattct cgattcaccc gacctgcatc
     2821 tcgatggctt ccttgggccc ggacacgtga gcatggtgat cggcacgcgg ccatatcgtt
     2881 ttattgcgga gcattaccgg cggccgatta ccgttgccgg attcgaaccg ctcgacgtgc
     2941 tgcaatcggt gtggatggtg ttgcggcaga ttgccgaagg ccgctgcgaa gtggaaaacc
     3001 agtacggcag gattgtgccg gaagacggta atgcgcaggc gctgtcggct atcgagcagg
     3061 tgttcgagtt gcgcgagttt ttcgagtggc gcggactggg atcgatagat cattcgggcg
     3121 ttcggatccg cgaacgcttc gccgcttacg atgccgagcg caagtttcct gtcgcgaacc
     3181 tgaagatcgc cgatcctaaa tcctgccagt gcggggaagt gctcaaaggc gtaatcaggc
     3241 cgcagcagtg caaggtattc ggcaccgcct gcacgccgga aacgccgctc ggatcgttga
     3301 tggtatcgag cgaaggcgcg tgcgcggcgc attatcgtta tgcacgcatc gatacatcgg
     3361 cgttgtacgc gcatggcgct gcgtcggaca cgctcaagca gggagtacag gcatgaacga
     3421 ccggatcggc aggttacgcg atgtcacgat caacctcgcg cacggcggcg gcggtcgggc
     3481 gatgcgcgat ctgatcgagg acgtctttgt caccgccttc gataaccccg cgctggccgc
     3541 gctcgaagac caggctgtgt ttccgctcgc ggacctcgca cgatcaggcg accggctcgc
     3601 gttcactacg gatagctacg tcgtcgaccc gttgtttttc ccgggcggag acataggtac
     3661 gttggcggta tcgggcacgg tcaacgacct tgccatgtcg ggggcaaaac cgctgtttct
     3721 atcatgcggc atggtgattg aagagggcct tgcggtagat gttctgcgcc gcgtggcggc
     3781 aagcatgcag cgtgtcgcaa tcgaagcggg tgtgtctatt gttaccggcg acaccaaggt
     3841 ggtcgagcgc ggttgcgcgg acaaactctt catcaacacc gcaggcgtgg gggtcgtgcc
     3901 gcccggcgta aatatatcgg cgcggcgcgc gcagccaggc gacgtggtaa tcgtgaacgg
     3961 ctttctgggt gaccacggcg cggcgatcct ggtcgcacgg aaacagcttg cattgcagtc
     4021 cgacattgaa agcgattgcg cgccgctcgc gtcactggtg gatgcaatgc tgggtgcgtg
     4081 caaggacatc cactgcatgc gcgacgcaac gcgcggcgga gtcgccaccg tgctgaacga
     4141 gttcgcggcg tcgtcaggtg tggcgatccg catccgggaa aacgcgctgc cgatccgcga
     4201 ggaagtgaag ggcgcttgcg agatcctggg cctggatccg ctgtatctgg cgaacgaagg
     4261 caagctggtt gcgatcgttc ccgcacatgc cgcatcaagc gtgctcgcgg cgatgcatgc
     4321 gcacccggct ggcgcgcggg cggcagtgat cggcgaggta gttgcgcatc ccgcgggcat
     4381 cgtggtgctg catacggtgt ttggtggaca gcgaatcgtg gacatgctga cgggcgagca
     4441 gctgccccgg atctgttaac tggtttcagg catggggggc gcagtgtcat gcatgaactg
     4501 agcatcgcga atagcgtcgt cgagctttgc gccgaacgcg cgatgggcgc gcgggtgctg
     4561 cgcgtgacgc tggagatcgg ccggctgtcg gcggtgctgc cggacgcaat ccgtttttgc
     4621 ttcgatgtct gcgcgaagga cacagtggtc gaaggcgcgg cgctggagat tgtcgagaca
     4681 ccgggacggg cgcgctgcct ggactgtggc agcaacgtgg agatgaccga cttgctgcaa
     4741 cactgcgcgt gcggaagcgt caatctcgaa ctgctgaccg gcagtgagct aaagataaaa
     4801 cagatggagg tggtgtgatg tgtaccacat gcggttgctc gaaaggtgcc ggctcggtga
     4861 ttacagatcc gcagaccggt gagcggattg agtcgcaggt agaagacgga agcggtgcgg
     4921 cgcgcaaacc gggttcgtgg cggcaacttg caggcggggc cgccggcggg agtgtggctg
     4981 cgcatctgca tgtgcgcgcg catcggcatg gtcatgcggc ccacatt
//
LOCUS       DATASH010000575         2751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5236884,
            whole genome shotgun sequence.
ACCESSION   DATASH010000575 DATASH010000000
VERSION     DATASH010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5236884"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="VHV99_15630"
     CDS             complement(<1..613)
                     /locus_tag="VHV99_15630"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEX3636889.1"
                     /translation="MRVFRGLPNAESRAPCALTIGNFDGVHRGHQALLAHVRAAADAR
                     GLPVCVMTFEPHPREFFNPAGAPPRIAMLRDKLEALRTNGVDRVVVEHFNHTFASQPP
                     DTFVENVIVNGLHARWVMIGDDFRYGAKRAGDFESLQGAGRKHGFEVEQMATVADLNG
                     ARISSSGVRSSLVAGDLDAASLALGRPYVISGHVVHGQKLGRDL"
     gene            826..1476
                     /gene="purN"
                     /locus_tag="VHV99_15635"
     CDS             826..1476
                     /gene="purN"
                     /locus_tag="VHV99_15635"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008351190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX3636890.1"
                     /translation="MKKIVILISGRGTNMETVVRACAREGWQAQVAAVISNRPDAAGL
                     AFAAANGITATVVDHRDFTTREAFDQALARSIDGFEPDLVMLAGFMRVLTDAFVERYA
                     GRMINMHPSLLPCFPGLKTHQQALDAGVRVHGASVHFVTPTLDHGPIVAQAAVPVMAG
                     DDAAALASRVLAVEHIIYPRAVRWFVEGRLAIDGERVVLTPPEPQWLFADIAGEGV"
     gene            1473..>2751
                     /locus_tag="VHV99_15640"
     CDS             1473..>2751
                     /locus_tag="VHV99_15640"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEX3636891.1"
                     /translation="MRLHGFLIGQTETLLADVLRFSGPADAATSRFFRAHPKLGHNER
                     GVIAEAVFAVLRRKMEFSHLAESGSGNLARRLALLGLMQTAGLSAVKPFISADEYQWL
                     NQVSKIDPGSLPVRVRTNLPQWIYDAMTKRFDTEELALLAAALNYPAPLDLRANPIKA
                     TRELVIKTLTEAGIDAGEMPFAPYGVRVDGKPALTRLQPFQDGWIEVQDEGSQLLCSL
                     VAPRRGEMIVDFCAGAGGKTLALGAMMRSSGRLYAFDVSDRRLAKLKPRLARSGLSNV
                     NPVLIDSEHDAKIKRLAGKIDRVLVDAPCSGLGTLRRNPDLKWRQSPESVAELTPKQL
                     SILTSAARLVKVGGRLVYATCSMLEAENEGVVNQFLAAHPGFKLVPAREVLAEQRIDL
                     DTGDYLQLWPHKHGTDGFFAAVLERLPVAAKEAA"
BASE COUNT          502 a          840 c          910 g          499 t
ORIGIN      
        1 cgagatcgcg gccgagcttt tgcccgtgga ccacatggcc gctgatcaca taagggcggc
       61 cgagtgcgag gctggcggcg tcgagatcgc ctgcgaccag cgacgaccgc acgcccgaac
      121 tcgaaatgcg cgcgccgttc aggtccgcga cggttgccat ttgttcgact tcgaagccgt
      181 gcttgcgccc ggcaccttgc agcgattcga aatcgccggc gcgtttcgca ccgtagcgga
      241 aatcgtcgcc gatcatcacc cagcgtgcat gcaacccgtt cacgatcacg ttttcgacga
      301 acgtatccgg cggctggctg gcgaaggtgt gattgaaatg ttcgaccacg acgcgatcca
      361 cgccgttcgt acgcagcgct tccagcttgt cgcgcagcat ggcgatgcgc ggcggcgcgc
      421 cggcaggatt gaagaattcg cggggatgcg gctcgaaggt catcacgcag accggcaggc
      481 cgcgggcgtc cgcggcggcg cgcacgtggg caagcagcgc ctgatggcca cggtggacac
      541 cgtcgaaatt gccgatggtc agcgcgcagg gcgcgcggct ttcggcattg ggtaggccgc
      601 gaaagactct cacgatatcg gttgggtcgg cggttggcgg ttaaatgggc tcaaatcagg
      661 gctcaaaaca agctgaatac agtcccgcag ggctcacgca gcgcgaggct ggcacagccg
      721 caaagcgttc gattataaac gctcacagca caggacggag gccgggccca tgacggggcg
      781 gcggattcgc cgggttttga cagcatcgaa tgataaaatc cgcggatgaa aaaaatcgtc
      841 atcctgattt ccgggcgggg aaccaatatg gaaaccgtcg tgcgggcttg cgcgcgcgaa
      901 ggctggcagg cgcaggtggc cgctgtcata tcgaatcgtc cagacgccgc cgggcttgcg
      961 ttcgcggcgg cgaacgggat tacagcaact gttgtagatc accgtgattt caccacgcgc
     1021 gaagccttcg atcaggcgct tgcacgcagt atcgacggct tcgagccgga cctcgtcatg
     1081 cttgcaggct tcatgcgcgt attgaccgac gctttcgtcg agcgctacgc gggccgcatg
     1141 atcaatatgc acccgtcgct gctgccgtgc tttccgggcc tcaaaaccca ccagcaagcg
     1201 ctcgatgccg gcgtgcgcgt gcacggcgca agcgtccatt ttgtcacgcc gacgctggat
     1261 catgggccta tcgtcgcgca ggcggcggtg ccggtcatgg ctggcgacga cgctgcagca
     1321 ctcgcttcgc gggtgctggc ggtcgaacac attatttacc cacgcgcggt gcgctggttc
     1381 gtcgaagggc gtcttgccat cgacggcgag cgtgtcgtgc tcacgccacc agagccgcaa
     1441 tggctctttg ccgacattgc cggggagggc gtatgaggct tcatggattt ttgataggac
     1501 agacagagac gttgctggcg gacgttctgc gcttcagcgg ccccgccgac gcggccacca
     1561 gccgcttttt ccgcgcgcat ccgaagctcg gccacaacga gcgcggtgtg atcgccgaag
     1621 cggtgttcgc cgtcctgcgc cgcaagatgg agttttcgca tcttgccgaa agcggcagcg
     1681 gcaacctcgc acggcgtctg gccttgctgg gcctgatgca gacggcgggt ttgtcggcgg
     1741 taaagccgtt catttccgcc gatgaatacc agtggctcaa ccaggtttcg aagatcgatc
     1801 cgggcagctt gccggtgcgg gtgcggacca acttgccgca gtggatctac gacgccatga
     1861 cgaagcgctt cgataccgaa gagctggcgt tgctcgccgc cgcgctgaac tatccggcgc
     1921 cgctcgatct gcgtgcgaac ccgatcaagg ccacgcgcga actggtcatc aagacgctga
     1981 ccgaagcggg tatcgatgca ggggaaatgc cgttcgcgcc ttacggcgta agggtggatg
     2041 gcaagccggc gctcacgcgc ctgcaaccgt tccaggacgg ctggatcgaa gtgcaggacg
     2101 aaggcagcca gcttttgtgc tcgctggtgg cgccccggcg cggtgaaatg attgtcgatt
     2161 tctgcgccgg cgcgggcggc aagacgctgg cgctcggcgc gatgatgcgc tcgtcgggcc
     2221 gtctctacgc tttcgacgta tccgaccgcc ggctcgcgaa actcaagccg cggctggctc
     2281 gcagcggttt gtcgaatgtg aatccggtgc tgatcgacag cgaacacgac gccaagatca
     2341 agcgcctggc cgggaagata gaccgcgttc tcgtcgatgc cccatgttcc ggcctcggca
     2401 ccctgcggcg taatccggac ctgaaatggc gccagtcgcc ggaatcggtg gccgaactga
     2461 cgccgaagca gttgtccatc ctgaccagcg cagcgcggct cgtgaaggtg ggcggccgtc
     2521 tcgtctacgc aacatgcagc atgctggaag cggaaaacga aggtgtggtc aatcagttcc
     2581 tggccgcgca tccgggcttc aagctggttc cggcgcgcga ggtgctggct gagcaacgaa
     2641 tcgacctcga tacgggcgac tatctgcaac tttggccgca caagcacggc acggatggtt
     2701 tcttcgccgc cgtacttgaa cggttgccgg ttgctgccaa ggaagccgca g
//
LOCUS       DATASH010000576         7191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_887124,
            whole genome shotgun sequence.
ACCESSION   DATASH010000576 DATASH010000000
VERSION     DATASH010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7191
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887124"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..200)
                     /locus_tag="VHV99_15645"
                     /pseudo
     CDS             complement(1..200)
                     /locus_tag="VHV99_15645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067469.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
     gene            complement(414..1415)
                     /locus_tag="VHV99_15650"
     CDS             complement(414..1415)
                     /locus_tag="VHV99_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3636892.1"
                     /translation="MDTLQNMRVFVRVVEAGSFTAAAQSLNSTTGAMSRAVSELEAHL
                     RTRLLNRSTRRLALTTAGERYLKRSQQILADVDTAEEEASCAHERPSGALRMHSFASI
                     GQHYVLPAISRYRALYPEVTVELTLSQRMPDLFEGSADVAVISASTLPNSDLVSLLLG
                     TTFSILCASPTYVRAHGAPQRPSDLAHHECLILHTPAFPPHEWVLDGPHGSEVMEVNG
                     PVQVNIAESLIVAIREGMGIGMVPLYAAIAGLRDGTLIRVLPEYTLQQTNVYALYPSR
                     KFIDAKTRTWVEFLRMHLPKMIARDEALLAEVGQMYTADGVAPVGAANIEDGDVAAH"
     gene            1618..3783
                     /locus_tag="VHV99_15655"
     CDS             1618..3783
                     /locus_tag="VHV99_15655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEX3636893.1"
                     /translation="MKRQHAIKPTADSRRQWSVMLPTLSAAIRDWANSDGLIGLHLLK
                     TVAAGLLALGIAMLLDLQQPRIAMTTVFVLMQPFSGMVLAKSFYRILGTAVGTAAALT
                     LGALFVQQPELYMLGMIGWVSACIAAAVRYRHFRWYGFVLAGYTAALIGIPNVTAPHN
                     LFLAALTRAAEVAVGIVCSSAVSALVVPQRSSLALRRALHVRYINFTAFAAEVLGRGI
                     ERGQFERRFADLVDEIVGFEATRTFAAFEDPAMRSRSQHLGRLNGEFMDACARLHALH
                     QLLKRLRVNGLSRIVDAITPYFHELSELLASTQDANADAARVAAGLRLFQTSLPRRVR
                     ETRRPLEAAPAEPLADFDTAAELLYRFVDEWTRYSETYASLARRKPGAEPRTPGGYVS
                     KTNGFVVAFAFARSAVVMAIAGWFWIATDWPSGGLAVIGAALVCALTSTAPNASKMAV
                     QMAVGVVLATMTGYLFTCYVYPSIDGFPLLCAALTPVLAFGAFIASRKRAAGYGIGFS
                     VFFCLLAGPDNVVTYAPDLLLNNGIAITASLLLAAIVFAVVFPADMPWLTERIKGDLR
                     AQVVSACKDELPGLNQRFQSSTHDLTSQLRMLLTRRSRRRRDALRWMLVTLEVGHAVI
                     DLRNEAARASYAHALHSRWGSSLAQTEGDLARLFTRPDSYALERALISVRSTTWIVQD
                     VLETVHADRDRRHDLQRILSCLHFIRTALLDKDAPFNAR"
     gene            3953..4168
                     /locus_tag="VHV99_15660"
     CDS             3953..4168
                     /locus_tag="VHV99_15660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636894.1"
                     /translation="MNVLKIALIACSLSAVVAHAQTAPAAPEQQIAQAKAPRANSVAE
                     QNRGAVPKAKADECVGPVSFCNIYFGS"
     gene            4357..5871
                     /locus_tag="VHV99_15665"
     CDS             4357..5871
                     /locus_tag="VHV99_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HEX3636895.1"
                     /translation="MYQDRIRCSALRGKITSAAEAALLIQDGMHVGASGFTRAGDAKA
                     VPVALAERVRQEGKPLQITLMTGASLGHDVDRMLTEAHVLARRLPFQVDKTLRDAINR
                     GEVMFVDQHLSETVEMLRANQLGKLDVAIIEAAAITETGGIVPTTSVGNSASFAILAD
                     KVIVEINLAQPLALEGLHDIWIPGRRPNREPLPIVRPQDRVGAQAIEIPLDKIAAIVI
                     TDKADSPSTVLPADPETELIAGHLIEFFQHEVSRGRMPRHLPPLQAGIGTIANAVLAG
                     FADSPFGTFEIYSEVLQDSTFDLMDAGKVTFASGASITLSAARQAQVFGELERYRDRL
                     VLRPQEVSNHPEVIRRLGLIALNTALEFDIYGNVNSTHVGGTHMMNGIGGSGDFARNA
                     SCAIFATKSMAKGGRISSVVPMVPHCDHNEHDVDVVVTEQGLADLRGLAPRERAMLII
                     ENCAHPLYRDLLRDYYRDALQWGGQTPHQLDQAFAWHRRLRDTGSMLPAVQALL"
     gene            complement(5937..6191)
                     /locus_tag="VHV99_15670"
     CDS             complement(5937..6191)
                     /locus_tag="VHV99_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX3636896.1"
                     /translation="MEHGIIAWLIIGAIAGWLAGVLVKGGGFGLIVDIIVGIVGAFIG
                     GWLAGVLHISLGGGWIGSIITAVIGAVILLFLIRLVRRGT"
     gene            complement(6356..7063)
                     /locus_tag="VHV99_15675"
     CDS             complement(6356..7063)
                     /locus_tag="VHV99_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006396758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEX3636897.1"
                     /translation="MTLEFFLRLFTAFACGVAIGLERQMRQRNAGLRTITLVASGACL
                     FVTLGVLTGNGVSGITQIAAYVVSGVGFLGGGVIMRDKGSIQGINTAATLWCSAAVGV
                     LSGAGHYGPALAGTAVVLLTNTVLREVSQMINSTSVSNADLVREYLLMIVCREADEIH
                     IRTAISNSMYSAPLSFQSLTSEDVDDEPGRIRVTATLKLHPKDQSKLEQMASRLSMEK
                     SVSSVSWTAREAEPTPE"
BASE COUNT         1375 a         2231 c         2163 g         1422 t
ORIGIN      
        1 gcccgtccaa cgtccgcgcc gtcacggctt acacgcacgg cgccttccca atacaccgcg
       61 ccggtcgact ggcgggaatc gagctcctgg tcgtccatca acggatcgag gcgccacgta
      121 agcgtgccgg ttttaatcgt catcgagacg ggatacggcg tgttcgtgcg tggcgagcgc
      181 catgtgcgaa ccggagtgaa gtgacttgct cgtcactatg gctggatctg gaaacatcgc
      241 gcgacgttgc ctatacaccc caatcttccg gcgctcactc ctttccgatc gaaaaaattg
      301 ccgctccctg gctctctgca gacccttggt gcaagcacaa atgggctgcc acttcgctaa
      361 tcacgtcgct gctttaccca ggcgcggtct ctgcacttcg tcttcaatct gtctcagtgc
      421 gcagcgacgt ccccgtcctc gatattagcc gcccctacag gcgcgacgcc atcggctgta
      481 tacatctggc cgacctccgc aagcaacgcc tcatcgcgtg caatcatctt cggcagatgc
      541 atgcgtaaga actcgaccca cgtgcgcgtc ttcgcgtcga taaacttgcg tgagggatac
      601 agtgcgtaaa cgttcgtctg ctgcagcgta tattcgggca acacgcggat taatgtcccg
      661 tcacgtaacc cggcaatcgc cgcatagagc ggcaccatac caatgcccat tccttcgcga
      721 atcgcgacga tcagcgattc ggcgatattc acctgcacgg ggccgttcac ttccatcacc
      781 tcgctgccgt gcggcccgtc gagcacccat tcgtggggcg gaaatgcggg tgtgtgcaga
      841 atcaaacact cgtgatgggc caggtcgctg ggccgttgcg gcgcgccatg agcgcgcacg
      901 tacgtaggcg atgcacacaa gatgctgaac gttgtaccca gcaggagcga tacgaggtcc
      961 gaattgggca aagtcgaggc gctgatcacg gcgacgtcgg cactcccttc gaacagatca
     1021 ggcatccgct gcgataacgt cagctcaacg gtcacttcgg gataaagcgc tcgataccgg
     1081 gaaatagccg gcagcacgta atgctggccg atgctggcga agctgtgcat gcgcaacgcg
     1141 ccgctggggc gttcgtgggc acagcttgct tcttcttccg cagtgtcgac atctgcaagg
     1201 atttgctgtg atcgcttcag gtaacgctcg ccagctgtgg tgagcgcgag gcgccgcgtc
     1261 gaccggttca gcaggcgcgt acgcagatgc gcttcgagtt ccgagacggc gcgggacatc
     1321 gcgcctgtcg tcgagttgag cgactgcgcg gcggcagtaa aactacccgc ttcgaccacg
     1381 cgcacgaaca ctcgcatatt ttgtaacgta tccatcgatc cttctaattc acggcagaag
     1441 ccgtattgtc cgcctgtccc gcaagctcat tgttgttcgt tctggaagag acgttccgca
     1501 gccaacctat taatgcgcgc agcgcatgct cctacaatcg gcgccttgcc tgccggtgcg
     1561 tgcagcagcg ttcaccggcg cttctatcag cgacaaaccg gggcgcgctg ctttccaatg
     1621 aaacgccaac atgcgatcaa accgacggcg gattcccgcc gccagtggag cgtcatgttg
     1681 ccaacgctca gtgcggccat tcgcgactgg gcgaacagcg acggtctgat cgggctgcat
     1741 ctgctgaaaa cggtcgccgc gggtttgctg gcgctgggta ttgcgatgtt gctggatctc
     1801 cagcagccgc gcatcgcgat gaccacggtg ttcgtgctga tgcagccatt cagtggcatg
     1861 gtgctcgcca agagtttcta tcggattctc ggcaccgcgg tgggcacggc cgcggccctg
     1921 acgctcggcg cgctattcgt ccagcaaccc gagttataca tgctcggcat gatcggctgg
     1981 gtgagcgcct gtatcgccgc ggcggtccga tatcggcatt tcagatggta cggttttgtg
     2041 ctggcgggtt ataccgctgc cctgatcggc attccaaatg tcacggcgcc tcacaacctg
     2101 tttctcgcgg cgctcacgcg cgcggcggaa gtggcggtcg gcatcgtgtg ctcgagcgcg
     2161 gtgagcgcgc tagttgtgcc acagcgttcc agccttgcat tgcggcgcgc gctccacgtt
     2221 cgatatatca attttactgc gttcgctgcc gaggtgctgg gtcgtggcat cgagcgcggc
     2281 cagtttgaac ggcgctttgc ggacctcgtc gacgagatcg tcggctttga agcgacgcga
     2341 acatttgctg ccttcgaaga tccggccatg cgttcacgca gccagcatct cggccgtctg
     2401 aacggcgaat tcatggacgc gtgcgcgcgg ctgcatgcac tgcaccaatt gctcaaacgt
     2461 ctgcgcgtga acggcctcag ccgaatagtc gatgccatta cgccgtactt ccacgagctg
     2521 tcggaattgc tggcatcaac ccaggacgcg aacgctgacg cagcgcgcgt ggccgccggt
     2581 ctacggctct tccagacgag cttgccacgg cgcgtgcgtg aaacgagacg gccgttggaa
     2641 gccgcacccg cagaaccgct ggcggatttc gatacggctg cggaactgct atatcgtttt
     2701 gtcgacgaat ggacccgtta ctccgagact tacgcatcgt tggcgcggcg caagcccgga
     2761 gccgagccgc gcacgcccgg cggctacgtg agcaagacga acggctttgt ggtcgcgttt
     2821 gcctttgcca ggtcggcggt cgtcatggcg atcgccggct ggttctggat cgccaccgac
     2881 tggcccagcg gcggcctggc ggtaatcggc gcggcgcttg tctgcgcgtt gacgtcgacc
     2941 gcgccgaacg catcgaaaat ggccgtgcag atggcagtcg gcgtagtgct ggcgaccatg
     3001 accggctatc ttttcacgtg ctacgtctat cccagcatcg acggttttcc gctgctttgc
     3061 gcggcgctta ccccggtgct tgcattcggc gcgttcatcg catcgcgcaa gcgcgcggca
     3121 gggtatggca tcggcttttc ggtgttcttc tgcctgctcg ccgggccgga caacgtcgtc
     3181 acgtatgcgc ccgatctgct gctcaacaac ggcatagcga tcacggcctc gctcctgctc
     3241 gcggcgatcg tgttcgcggt ggtttttccc gcggatatgc cgtggctcac cgagagaatc
     3301 aagggcgatt tgcgcgcgca agttgtgtcc gcatgcaagg acgaactgcc cggcctcaat
     3361 cagcgctttc agtcgagcac ccatgatctg acctcgcagt tgcgcatgct gttgacacgg
     3421 cgctcccgcc gccgccgcga tgccttgcgc tggatgctcg tcacgctcga agtagggcac
     3481 gccgtgatcg atctgcgcaa tgaagcggcg cgtgccagct acgcgcatgc gcttcattca
     3541 cgctggggca gcagcctcgc ccagaccgaa ggcgacctcg cgcggctctt cacgcgtcct
     3601 gactcgtatg ctctcgagcg ggccctcata tccgtgcgct cgaccacgtg gatcgtgcaa
     3661 gacgtcctgg aaaccgttca tgccgaccgc gacaggcggc atgacctgca gcgcatcctc
     3721 agttgtctgc acttcatccg cacagcgttg ctcgataaag acgcgccgtt caatgcgcgc
     3781 tgacccatgc gcgttggatc agacgcgcgc cccgatcttt ctgccagatg gaatgatcac
     3841 ttccgcccag cccggtttat ccacgtaggt ggcagtcata tagtttcacc actgccacgc
     3901 agtctcccga ggggcttcct gcggggcgga accgtaagta ctggagaacg aaatgaacgt
     3961 gttgaagatt gccttgattg cctgctcttt gtccgctgtt gtcgctcatg cacagaccgc
     4021 accggccgcc ccggaacaac agatcgcgca agcgaaggca ccgcgcgcca acagcgtcgc
     4081 cgagcaaaat cgcggcgctg taccgaaggc caaggctgac gaatgcgtcg ggcccgtgag
     4141 cttctgcaat atctacttcg gcagctgaac agcgccacga gcaacatcag cagtaccgca
     4201 ggatcttcgc gggaatttaa cgcgacgaaa catctggccg cgacgccgtc ccatcgagac
     4261 accgtggctg gttcgacaat ctggctactg agcgaatcac accgcagacg tgcgcaccgg
     4321 tagaccggac tggtgactat ggagagactc agtttcatgt atcaagaccg gattcgttgc
     4381 tccgcgctcc gcggaaaaat cacgtcagcc gccgaggcgg cgcttctgat tcaggacggc
     4441 atgcacgtcg gcgccagcgg ctttacgcgc gcgggcgacg ccaaagctgt ccctgtcgca
     4501 ctcgccgagc gggttcgtca ggaaggcaag ccactgcaaa ttacactgat gaccggcgca
     4561 tcgcttggcc atgacgtgga tcgcatgctc accgaggcgc atgtattagc gcgccgcttg
     4621 ccgtttcagg tcgacaagac cctgcgcgac gcgatcaatc gcggcgaagt catgttcgtc
     4681 gatcagcatt tgtccgaaac cgtcgagatg ttgcgcgcaa atcaactcgg caaactcgac
     4741 gtcgccatta tcgaagctgc cgcgatcacc gaaaccggtg gcatcgtgcc gaccacttca
     4801 gtcggtaact cggccagctt cgcgatcctc gccgacaagg tcatcgtcga aatcaatctc
     4861 gcccagccgc tcgctctcga agggctgcac gacatctgga ttccgggccg caggccgaat
     4921 cgcgaaccgc tgccgatcgt gcggccgcaa gaccgtgttg gcgcgcaggc catcgagatt
     4981 ccgctcgaca aaatcgcggc gatcgtgatt accgataagg cggacagtcc gtccacggta
     5041 ctgcccgcgg atccggaaac cgaattgatc gcaggccatc tgattgagtt tttccaacac
     5101 gaagtgtcgc gcgggcgcat gccccggcac ttgccgccgt tgcaggcggg tatcggcacg
     5161 attgccaatg cggtgctggc cggcttcgcc gattcgccct tcggtacgtt cgagatttac
     5221 tcagaagtac tgcaggattc gaccttcgat ctgatggacg ccggcaaggt gacgttcgcc
     5281 tcgggtgcat cgatcacgtt gtcggccgcg cgtcaggcgc aggtgttcgg cgaactcgag
     5341 cgctatcgcg atcgcctggt gctgcgtccg caggaagtca gcaaccatcc tgaagtgatt
     5401 cgccggcttg ggttgatcgc gttgaatacc gcactggagt tcgacattta cggcaatgtg
     5461 aattccaccc acgtgggcgg cacgcacatg atgaacggca tcggtggttc gggcgatttt
     5521 gcacgcaatg cgtcatgcgc gatcttcgcg acgaagtcga tggcgaaggg tggccgcatt
     5581 tcaagtgtcg tgccgatggt cccgcactgc gatcacaacg aacacgatgt ggacgtggtc
     5641 gtaacggagc agggcttggc cgacctgcgc ggcctcgcgc cacgagaacg cgcgatgttg
     5701 atcattgaga actgcgcgca tccgctctat cgcgatctgc ttcgcgacta ttatcgggac
     5761 gccttgcaat ggggtgggca aacgccgcat cagttggacc aggctttcgc ctggcatagg
     5821 cggctgcgtg acaccggctc gatgttgccg gcagtgcaag cgcttctcta aacttcaggt
     5881 aaaaccggcg tcaggtggca ttgacccccg acgccgcaag acatcaggtg ctacgctcag
     5941 gtaccgcgcc ggacaagccg gataagaaac agcagaatga cggcgccgat cacagcggta
     6001 atgatcgaac cgatccagcc gccgccgagc gaaatgtgca acacgccggc cagccagccg
     6061 ccgatgaacg caccgacaat cccgacgatg atgtcgacga tcagaccgaa gccgccgccc
     6121 ttgacaagca cgccagccag ccagccggcg attgcgccga tgatgagcca tgcaatgatg
     6181 ccgtgttcca taatcgagac tccatggttg agtgtgaaaa attcacggtg acagagctta
     6241 gtcgaaggtt atgggacagt ccatattcag gacgcggaat taacattgtc taaatctatt
     6301 gcaagctaag tgtagacgat gcgcgcgaat atggggggag cttacgcgtg gcgactcact
     6361 ctggcgtcgg ctccgcttct cgcgcggtcc aactgacgct ggacacgctc ttttccatgc
     6421 tcaggcgact tgccatctgt tcgagcttcg attgatcctt cggatgcagc ttcaacgttg
     6481 ccgtgacgcg aatgcggccc ggctcgtcat cgacatcttc gctcgtcagg ctctggaacg
     6541 acagcggcgc cgagtacatc gagttcgaaa tcgcggtgcg aatgtgaatc tcgtcggctt
     6601 cgcggcagac gatcatcagc aggtactcgc ggacgaggtc ggcgtttgaa accgatgtcg
     6661 agttgatcat ctggctcact tcgcgcagca cggtgttggt cagcagcacg accgcggtgc
     6721 ccgccagcgc cggtccgtaa tgtccggcgc cgctcaacac gccgacggcc gccgagcacc
     6781 acagtgttgc cgcggtgttg attccctgaa tcgagccttt gtcgcgcatg atgacgccgc
     6841 cgccgagaaa gccgacgccg gacaccacat acgcggcaat ctgcgtgatg ccgctgacgc
     6901 cgttaccggt cagcacgcca agcgtgacga acaggcatgc gccgctcgcg acgagcgtga
     6961 tcgtgcgcag gccggcattg cgctggcgca tctgccgttc gaggccaatc gcgacgccgc
     7021 aagcaaaagc ggtgaaaagc cgcaggaaga attcgagtgt catcgttcag aaaggttggc
     7081 gtgcgtgctg ccgcagggct aagcctacgc gtgacacaag ctaatctcgt ctacctcgtg
     7141 agggagtgct cggagcgtgc ggaagaaacg agagcgacgt gactaatgcg g
//
LOCUS       DATASH010000577         5488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1086347,
            whole genome shotgun sequence.
ACCESSION   DATASH010000577 DATASH010000000
VERSION     DATASH010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5488
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1086347"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..953
                     /locus_tag="VHV99_15680"
     CDS             <1..953
                     /locus_tag="VHV99_15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HEX3636898.1"
                     /translation="NLAAALIVGLGCERNQLAGLMDQEGLKADSRLHTFIMQESGGTR
                     RTIEAGVAAVQALLPDANRVKRTTVPASHLKIGLQCGGSDGFSSITANPSLGAAMDLL
                     VRHGGTAILSETPEIYGVEHTLTRRAVSREVGEKLVERIHWWKDEYSVGRDVQINGQV
                     SPGNQVGGLANIFEKSIGSAMKGGTGPLMQVYRYAELVKQPGLVFMDTPGFDPVSATG
                     QIAGGANLIAFTTGRGSMFGAKPVPSLKLATNTPMYTRLTEDMDLNCGGILDGTASIQ
                     STGQKIFDAILAAASGERTKSEMLGLGDHEFVPWQIGIMS"
     gene            1055..2362
                     /locus_tag="VHV99_15685"
     CDS             1055..2362
                     /locus_tag="VHV99_15685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013691278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3636899.1"
                     /translation="MSTQNPAIQVPDSAASNSIYTRVTWRFMPLLFVCYVVAYLDRVN
                     VGFAKLQMLSDLNFSEAMYGFGAGIFFIGYFFFEVPSNLLLHRLGARRWIARIMLTWA
                     VLSAATAWVSTPTMFYVLRFLLGVAEAGFFPGLVLYLTYWFPSHRRGKMIAFLMAGNP
                     VAGIVGGPISGFIMQRLAGNAGLTGWQWLFILEALPALILAAVIFWFLDDRVKDAKWL
                     PVQDRDFIAAEIDAEDRTKSHGSVREVFTSLKVWTLCTILFGIVMGSYAVGFWQPTII
                     KSSGITDPFTIGLLTVVPYLSALILMIVVGRHADRTRERRWHVVLPQCMAAVGFVMCA
                     YAGNNTYIALLGLTLAASGVITALPMFWALPTSFLGGTGAAAGIALINSTGNLAGFVS
                     PALVGWLKTVTHSLDSSLFLIAGSLIISACLILTQLPARLVNK"
     gene            2434..3726
                     /locus_tag="VHV99_15690"
     CDS             2434..3726
                     /locus_tag="VHV99_15690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016347180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3636900.1"
                     /translation="MKTGKYRYIIALLLFIAGAINYMDRAALGVVAPILNKELNLSPS
                     QLGVVFSSFFFGYSIFAFVGGQLADKYGPRRVFSWAMGTWSILCGLTAAATGFGTLLV
                     FRALFGFGEGPMNSTTNRTITNWFPRDETATMVGFTFSGQTVGSAIAGPVVGLIAIAY
                     GWRTSFVIIAALGLVWIVAWRIFATDKPAQSARVGEAEREMVEASRSAAHPAEGGDAS
                     TPLRSYLFRPSTLALGVGLFAVNYTLFVFISWMPSYFTNALHLDMKHMSILSAVPWAC
                     GGIGYFGGGLVADAFFKRMSNKLLARKVAAIVPLALSGLALVGVSMVDSVTPAVLLVA
                     FAVLFLTASSQACWAIMHELVPGRHLGGVSGFVHLISNISGIVGPTMMGLAVQYFGGF
                     SSGFMLGAAVDLVGVLAMLLVIGRRTAGETVGTHTTAA"
     gene            3849..5015
                     /locus_tag="VHV99_15695"
     CDS             3849..5015
                     /locus_tag="VHV99_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX3636901.1"
                     /translation="MKHVIVESEQTDYQTRSRIVSVEARTIRVPLDRHTAFSTRLVTA
                     RDYTVVRVTTADGHHGIGFCYSGNRAGMLVTYAVRELFAPLLRGRSALDVEGSWCAMY
                     DESLLQGRAGSVMRALSILDIALWDRNARAAGLPLSRYLGGTCSATVPAYASGGYYLE
                     GKTPEHLGEEMAGYVALGFRAVKMKVGRLDPRSEEARIRAAREAIGDDVLLMLDANNA
                     WSDVPTALEYMRRYEPFNPYWIEEPFSPDDIESHAQLSRRTPVTVATGEIEAGRWRHQ
                     ELLDKRAAMILQSDAAVCGGISEWRRIAASAAAVGVTMCPHWFHDLHVHLVASTSNAR
                     YVEFFPDDQVLNFRRLVDTQLSFADGKLTVPTRPGLGFDFDEAALNEYALDEWA"
     gene            complement(5085..>5488)
                     /locus_tag="VHV99_15700"
     CDS             complement(5085..>5488)
                     /locus_tag="VHV99_15700"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX3636902.1"
                     /translation="WSNTQAMLERFQPHGFDVFAGSETFLLATMRAGGVGCITATGNV
                     NPAAIVQLYRHWQSADADARQAALDATRAVFARFPMIPALKAAIGVYSNDPDWGVVRP
                     PLVDLTPVQCDALAAALGEIGFSMPGVEVTA"
BASE COUNT          978 a         1656 c         1742 g         1112 t
ORIGIN      
        1 ccaatctcgc cgcggcgctg attgtcgggc tgggatgcga gcgcaaccag ctggccggac
       61 taatggatca ggaaggtctg aaggccgact cgcggctgca cacgttcatc atgcaggaga
      121 gcggcggcac ccgccggacc atcgaggccg gcgtggccgc ggttcaggcg ctgctacccg
      181 acgccaatcg cgtgaagcgc acgacggttc ccgcgagcca cctgaagatc ggtctgcaat
      241 gcggcggctc ggacggcttt tcgtcgatca ctgccaatcc ttcgcttggc gcggcgatgg
      301 acttgctggt tcgtcacggc ggcacagcca tcctctccga aacacccgaa atttacggcg
      361 tggaacatac gctcacgcgg cgtgcggtca gccgcgaagt tggcgagaag ctggttgagc
      421 ggatccactg gtggaaagac gagtattcgg tcggtcgaga cgttcagatc aacggccagg
      481 tcagccctgg caatcaggtg ggcggactgg cgaacatctt cgaaaaatcg attggatcag
      541 cgatgaaagg cggcaccggg ccgctgatgc aagtctatcg atacgccgag ctggtgaagc
      601 aacctggact cgtgttcatg gacacacccg gtttcgaccc cgtctccgcg acgggtcaga
      661 ttgccggcgg cgcaaatctt attgccttca ccacagggcg cggttcgatg ttcggcgcca
      721 agcccgtgcc atcgctgaag ctcgccacca acacgccgat gtacacccgt ctgaccgaag
      781 acatggatct gaactgcggt gggatcctgg acggcactgc gtcgatccag agcacgggcc
      841 agaaaatctt cgacgctatt cttgcagccg cgtcgggcga gcgcacaaaa agcgaaatgc
      901 ttgggctagg tgatcacgaa ttcgtaccgt ggcagatcgg catcatgagt tgagtccaca
      961 gtgactcagc gcatcgcgag ggcgaacggc catttatacc cataacgaaa accgccgccc
     1021 tgtagctagt cgaacaaaat agcggagaca gcccttgagt acgcaaaacc cggcaataca
     1081 agtacctgac agcgcggcaa gcaactccat ctacacgcga gtgacctggc gcttcatgcc
     1141 tttgctgttc gtctgctacg tggtggcgta tctggatcgg gtcaacgtgg gcttcgccaa
     1201 gctccagatg ctcagtgatc tgaacttcag cgaggccatg tacggcttcg gcgccggcat
     1261 ctttttcatc gggtatttct tcttcgaggt gccgagcaac ctgctgctgc accggctcgg
     1321 cgcgcgccgc tggatcgccc ggatcatgct cacgtgggcc gtgctctcgg ccgcgaccgc
     1381 gtgggtcagc acgcccacga tgttctacgt attgcgtttc cttctgggcg tggccgaagc
     1441 gggctttttc ccgggactgg tgctgtatct cacctactgg tttccatcgc accggcgcgg
     1501 caagatgatc gctttcctga tggcgggcaa tccggtggcg ggcattgtcg gcggcccgat
     1561 atcgggcttc atcatgcagc gcctcgcggg gaacgcgggg cttaccggct ggcagtggct
     1621 gttcattctg gaagccctcc ccgcgctgat cctggccgcc gtcattttct ggtttctcga
     1681 cgaccgggtg aaggacgcaa agtggctgcc ggttcaggac cgcgacttca ttgcagcgga
     1741 aatcgatgcg gaagaccgca ccaaaagcca cggttccgtg cgcgaggtct tcacgtcgct
     1801 caaagtctgg accttgtgca cgattctctt cggtatcgtg atgggctctt atgccgttgg
     1861 cttctggcag ccaaccatca tcaagagttc gggaatcacg gaccccttca cgatcggcct
     1921 gctcaccgtc gtgccctatc tctccgcctt gattctgatg atcgtggtcg gccgccacgc
     1981 ggaccgcacg cgtgaacgcc gctggcacgt cgtgttgccg caatgcatgg cggcggtggg
     2041 tttcgtgatg tgcgcctatg cgggcaacaa tacgtatatt gcgttgcttg gcctgacgct
     2101 tgccgcttcg ggcgtgatta cggccttgcc catgttctgg gctttgccca cgtcttttct
     2161 cggcggcacc ggtgcggccg ccggcatcgc attgatcaac tcgacgggca atctcgcagg
     2221 cttcgtaagc cccgcgcttg tcggatggct aaagacggtc acgcattcac ttgattcgag
     2281 cctgttcctg atcgcggggt cgctgattat ctccgcatgt ctcatcctga cgcagcttcc
     2341 cgccaggctt gtcaacaagt agtgcctcat ggcaccgcca gaagtgccag ataacaatat
     2401 tcggtatcct taattacatg gaatagagac aacatgaaga ccgggaagta tcgatacatt
     2461 atcgccttgc tgctttttat tgccggggcg atcaattaca tggaccgcgc ggcgcttggc
     2521 gtggtcgcgc ctatcctcaa caaggagctg aatctttcgc cgtcgcaact cggcgtggtg
     2581 ttcagcagtt ttttcttcgg ctattccatc ttcgcattcg tcggcgggca actcgccgac
     2641 aagtacggac cgcgccgggt cttctcgtgg gcgatgggga cgtggtccat tctctgcggg
     2701 ctgactgcgg ccgcgactgg attcggcacg ttgctggtct tccgggcgct gttcggtttc
     2761 ggtgaaggtc cgatgaattc gacgaccaac cggaccatca ccaactggtt tccccgggat
     2821 gaaaccgcga ccatggtcgg ctttacgttt tcgggccaga cggtgggcag tgctattgcg
     2881 gggccggtgg tcggactgat tgccattgcc tacggctggc gcacgtcgtt tgtgattatc
     2941 gcggcgctcg ggctagtatg gatcgtcgcc tggcgtatct tcgcgaccga caagccggcg
     3001 caaagcgcgc gggtcggcga ggccgaacgc gagatggtcg aagcgagccg tagcgcggcg
     3061 catccggcag aaggcggcga tgcaagcacg ccgctgcgca gctacctgtt tcgtccgagc
     3121 acgctcgcgc ttggtgtcgg ccttttcgcg gtgaactaca cattgttcgt gttcatctcg
     3181 tggatgccga gctatttcac caatgcgctg catctggaca tgaagcacat gagcattctg
     3241 agcgcagtgc catgggcatg cggcgggatc ggatatttcg gcggcggcct ggtcgccgac
     3301 gcgtttttca agcgcatgag caacaagttg ctggcacgca aggtcgcggc gatcgtgccg
     3361 ctcgcgctgt cgggtctcgc tctggtgggc gtgagcatgg tcgattccgt gacgcccgcg
     3421 gtactgcttg tcgcgttcgc cgtgctgttc ctgacagctt cgtcgcaagc gtgctgggcc
     3481 atcatgcacg agcttgtgcc gggcaggcat cttgggggcg taagcggctt cgttcacctg
     3541 atcagcaaca tttcgggcat cgtcggtccg accatgatgg gtttagcggt ccagtacttt
     3601 ggcggtttca gcagcggctt catgctcggc gcagcggtcg atctggtcgg cgtgctggcc
     3661 atgctgctgg tgatcggccg gcgcacggca ggcgagacag ttgggacgca taccaccgcc
     3721 gcataaacgg tccgtcctgc caggcatgct gtcagggcgg cgcaggcgag cacccgcccg
     3781 cgaccatgcg cgcggccgcc caatcggtcg cgtgagcatc gtcgtctttt tcgagtcgat
     3841 ctactgctat gaaacacgtc atcgttgaat ctgagcaaac cgattatcag acacgttcgc
     3901 ggatcgtttc ggtggaggcg cgcaccatcc gcgtgccgct cgaccgccac actgcattct
     3961 cgacccgtct cgtcaccgcc cgcgactaca cggtcgtgcg cgtcacgaca gccgacggcc
     4021 atcacggcat cggcttttgc tacagcggca accgcgccgg catgctggtc acgtacgccg
     4081 tacgcgaatt gtttgcgccg ctgttgcgcg ggcgctccgc gctcgatgtc gaaggctcgt
     4141 ggtgcgccat gtacgacgag tcgctgctgc aaggacgtgc cggctccgtc atgcgggcgc
     4201 tgtccatcct cgacatcgcc ttgtgggacc ggaatgcacg cgctgcgggt ctgccgttga
     4261 gccgctatct cggcggcaca tgtagtgcca ccgtgccggc ctatgccagc ggcggttatt
     4321 acctcgaagg caagacgccc gagcacctcg gcgaggaaat ggcgggctac gtggcactag
     4381 ggtttcgcgc cgtgaagatg aaggtagggc gccttgaccc acgcagcgag gaagcgcgga
     4441 tcagggcggc acgagaagcg atcggcgacg acgtgctgct gatgctcgat gccaacaacg
     4501 cctggtccga cgtgccgacc gcgctcgaat acatgcgccg gtacgagccg ttcaacccgt
     4561 actggataga agagccgttc agtcctgacg acatcgagag ccatgcgcaa ctgagccggc
     4621 gcacgccggt caccgtcgcg accggcgaga tagaagcagg gcgttggcgg catcaggagc
     4681 tgctcgacaa gcgcgcggcc atgatccttc agtcggacgc ggcggtatgc ggcgggatca
     4741 gtgaatggcg aaggattgcg gcgtcggccg cggccgtggg cgtgacgatg tgcccgcact
     4801 ggttccacga tctgcatgtg catctggtgg cgtcgacgag caatgcgcgc tatgtcgaat
     4861 tctttcccga cgatcaggtg ctgaatttcc gtcggctggt ggatacgcaa ctgtcgttcg
     4921 cggacggcaa gctgaccgtg ccgactcgtc caggcctcgg attcgacttc gatgaagccg
     4981 cgctgaacga atacgcgctg gacgagtggg cgtgaagccg aagcaatgaa tagctgaaaa
     5041 gaccaccggt tgcgcatgca accggcgggc gcttcttcag gcgatcacgc cgtgacttcg
     5101 acacctggca tcgagaagcc gatctcgccg agtgcggcgg cgagcgcgtc gcactgcact
     5161 ggcgtcagat cgacgagagg agggcggacg acgccccagt cgggatcgtt cgaatacaca
     5221 ccgatcgctg ccttgagcgc aggaatcatc gggaacctgg cgaacaccgc gcgcgtggca
     5281 tccagtgctg cctgacgggc atcggcgtcg gctgattgcc agtggcgata caactgcacg
     5341 atagccgccg gattgacgtt cccggtcgcc gtaatgcagc cgacaccgcc ggcccgcatc
     5401 gtggcgagca ggaacgtttc gctgccggcg aacacatcga agccatgcgg ctggaagcgc
     5461 tcgagcatgg cctgggtgtt gctccagt
//
LOCUS       DATASH010000578         3200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3263175,
            whole genome shotgun sequence.
ACCESSION   DATASH010000578 DATASH010000000
VERSION     DATASH010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3263175"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..743
                     /locus_tag="VHV99_15705"
     CDS             156..743
                     /locus_tag="VHV99_15705"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HEX3636903.1"
                     /translation="MNRIVVGLCCVLVSGVASAAANSGVVMIAETDVSAQEAATFPAD
                     VDIQVQSNARTQTAAGTSAGITVTASAVPELAARIRGLESSFRCLVCQNETIADSTAG
                     LAADLRMIIHEQVQQGATDAQIRDYMVTRYGDFVLYDPPFKTLTWVLWLGPFVLLAMG
                     LLAFAGVVLQHRKMTATPLTELQQQRVRRLLSDRP"
     gene            740..1978
                     /gene="ccmI"
                     /locus_tag="VHV99_15710"
     CDS             740..1978
                     /gene="ccmI"
                     /locus_tag="VHV99_15710"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HEX3636904.1"
                     /translation="MITFWIIAAVMLIAAVLCAVVPLMQRESATGAPKVRALSVSLYR
                     RELADADADLRTGALSPGQYASVRSDMEQRVLDDTAASPSLARRSTPRTATRAATCAV
                     LIALFPSAACLLYLQIGEPAALKLNNPLDDSLGVSHDMSSMSVEMMVSRLAFRLRDHA
                     PDPRDAAAWATLARSYTVLERPTDAVAAYAKAVAIEPGAAPLRADFADAIASADDGRF
                     AGRASEEIAAALAIDPADPKALALAASAAFDGRGYARAIGYWRRLLATLNADSPEAMK
                     VQKNIDEAEGLSAVTVDIRLADGAQVASDRTVIVTARTSDAPASTNPASAAIAVSVAV
                     PPNTLLAERRLSAGELPTTIVLDDSVAINATDTLSAHQQIVVEARIVLGSGGNDLTGR
                     GDAKGLENRHASVVIAAPSP"
     gene            2087..2836
                     /locus_tag="VHV99_15715"
     CDS             2087..2836
                     /locus_tag="VHV99_15715"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEX3636905.1"
                     /translation="MFEFEELLLIAYKKERATSRRLSKLTSLCAHAALTQTLEKSAGT
                     QTLAELVSARRRERAAAEQARHHREEERAAARAARKVQSAVRDAVAWYAWFDGSAHPN
                     PGKIGIGGVLEGPDGDVVKISARAGHGDSCEAEYLALIAVLEAAVSRRPAPAKLVIHG
                     DSQVVIEDVLRGEASSAPALSTYAAQARALIAQLADVRLQWIPRARNAAADALSQHAV
                     HEDTHAGTHSDHHDKSRTSTTGVSPNVSLNA"
     gene            complement(2776..>3200)
                     /locus_tag="VHV99_15720"
     CDS             complement(2776..>3200)
                     /locus_tag="VHV99_15720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018438419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3636906.1"
                     /translation="SVSLNVAAPLMLSAAVAAASPSASDRRIVHISSGAARNAYPGWS
                     IYCATKAALDHHARAVALDNNRALRICSVAPGVVDTDMQAEIRGTGLEHFPLREKFEE
                     LKRSGQLSTPADAARKVLDYALSDTFGDTPVVDVRDLS"
BASE COUNT          593 a          983 c         1013 g          611 t
ORIGIN      
        1 gacgggcatc gactatggcg tgtatggcgt gcctgaaacg tttgtcgtcg accggcgtgg
       61 tgtgatccgc ttcaaacgct cgggaccact gacacaagcc gcactcgaca cgcaaatcct
      121 gccgctcgtg cgaagccttc aaggcgaggg cagcgatgaa taggattgtg gtcgggttgt
      181 gttgcgtgtt ggtgagtggt gtggcgagtg cagccgcaaa ttccggcgtc gtgatgattg
      241 ccgaaaccga cgtcagcgct caggaagccg cgacctttcc cgctgacgtt gacatacagg
      301 ttcaaagcaa cgctcgaacg caaactgctg ccggcacgag cgccggaatc accgtgacgg
      361 ccagcgctgt cccggaactc gccgcccgca tccgcggcct tgaatcgagc tttcgctgtc
      421 tcgtctgcca gaacgaaacc atcgcggatt ccacggccgg tctcgccgct gatctgcgca
      481 tgatcatcca cgagcaagtc cagcagggtg caaccgatgc gcagattcgc gactacatgg
      541 tgacgcgtta cggagatttc gttctttacg atccgccgtt caaaacgctg acttgggtcc
      601 tttggctcgg tccattcgta ctcctcgcga tgggcctcct ggcgttcgca ggcgttgttc
      661 tgcaacaccg gaagatgacc gcaacaccgc tcaccgagct gcagcagcaa cgcgtcagac
      721 gtctgctgag cgaccggcca tgattacgtt ctggatcatc gcagccgtga tgttgatagc
      781 ggccgtgctg tgcgcagttg tgccgttgat gcagcgggaa agcgcaaccg gtgcgccgaa
      841 ggttcgcgcg ttgagcgtgt cgctgtatcg gcgggaattg gctgatgccg acgcggatct
      901 gcgcacgggc gcgttatcgc cgggccaata tgcaagcgtg cgcagcgata tggaacagcg
      961 cgtacttgac gataccgccg cttcgccttc ccttgcacgc aggtccacgc cgcgtacggc
     1021 tacacgcgcc gcaacctgtg ccgtgctgat tgcgcttttc ccgagcgctg cgtgcctgct
     1081 gtatctgcag atcggcgaac cggccgcgct taagctgaac aacccgctgg acgactcgct
     1141 gggcgtctcg cacgacatgt cgtcgatgtc ggtggagatg atggtttccc gtctcgcgtt
     1201 tcgtctgcgc gatcatgcgc cagatcctcg cgatgccgcc gcctgggcca cgctcgcgcg
     1261 ctcatacacc gtgctcgagc ggccgacgga cgcagtcgcg gcctacgcga aggcggtggc
     1321 gatcgagccg ggcgccgcgc cgctgcgggc cgattttgcc gacgccatcg ccagcgcgga
     1381 cgatggccgg tttgccgggc gagcatcgga ggagatcgcg gcagcgctcg ccatcgatcc
     1441 cgctgatccg aaagcgcttg cattggcggc atcggcggca tttgacgggc ggggttatgc
     1501 acgtgcgatc gggtattggc gaaggctgct cgcgacgctc aacgcggatt cgcctgaggc
     1561 tatgaaggtg caaaagaata ttgatgaggc ggagggcttg agcgcggtta ctgtggatat
     1621 tcgactggcc gatggcgcgc aggtcgcgtc cgatcgaacg gtaatcgtga cggctcgcac
     1681 gagcgacgca ccggcttcga cgaatccggc gagcgcggcg attgcggtca gcgtggcagt
     1741 cccgccgaat acgttgcttg ccgagcgccg gctgtcagca ggtgaacttc ccactacgat
     1801 cgttcttgat gactcggttg cgatcaatgc aaccgacacg ctctcggcgc accagcagat
     1861 tgtggtggaa gcgcggattg tgttgggatc gggagggaac gacttgaccg gacgaggcga
     1921 cgccaaggga ttggagaatc gacacgccag cgtggtgatc gcggcgccat cgccatagac
     1981 gcttcatatc aaccggcaac gccctcagcg ctccgatttt cgacccgctg caaaaacttg
     2041 ttaagctcgg cattctatca cttcattcga tcacttaatg cccggcatgt tcgagtttga
     2101 agagctattg ctcattgcct acaagaagga gcgtgcgaca agccggcgct tgtcgaagct
     2161 cacttcgctc tgcgctcacg ccgccttgac gcagacgctg gaaaaatccg ccggcacgca
     2221 gacgcttgcg gaactcgtga gcgcgcggcg tcgggaaaga gctgcggcag aacaggcgcg
     2281 acatcatcgt gaagaagaac gcgcagccgc gcgcgcggct agaaaagtgc aatccgctgt
     2341 ccgtgatgcg gtcgcctggt acgcctggtt cgacggttcg gcccatccga atcctggcaa
     2401 gatcggcatt ggcggtgtgc tggaaggccc ggacggcgac gtcgtgaaaa tcagcgcgcg
     2461 ggccggtcat ggcgacagtt gcgaggcaga atatcttgcg ctgatcgcgg tgctcgaagc
     2521 cgcggtatca cgaaggccgg cgccggccaa gttggtgatt catggcgaca gtcaggtcgt
     2581 cattgaagac gtgcttcgcg gcgaagccag cagtgcgccc gcgctaagca cctacgccgc
     2641 gcaggcgcgc gctttgatcg cccagctcgc cgatgttcgc cttcagtgga ttccccgcgc
     2701 ccgcaacgcc gccgccgatg cgctttcgca gcacgccgtt cacgaggata ctcatgcggg
     2761 cactcactca gaccatcacg acaaatcgcg tacgtcgacc acgggcgtgt cgccgaacgt
     2821 atcgctcaac gcgtagtcga gcacttttcg cgcagcgtcg gccggggtgg aaagctggcc
     2881 gctgcgtttg agttcttcaa acttctcccg caacggaaag tgctccagac ccgtgccgcg
     2941 aatctcggct tgcatgtcgg tatcgaccac acccggtgca acgctgcata ttctcaacgc
     3001 ccggttgtta tccagcgcga ccgcgcgcgc gtgatgatcg agcgctgcct tcgtcgcgca
     3061 atagatgctc cagccgggat acgcattgcg ggctgcgccg ctcgagatat gcacgatacg
     3121 tcgatcagaa gccgatgggc ttgctgccgc cactgctgcc gacaacatca atggcgccgc
     3181 aacattcagg ctcaccgatc
//
LOCUS       DATASH010000579         1706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4250525,
            whole genome shotgun sequence.
ACCESSION   DATASH010000579 DATASH010000000
VERSION     DATASH010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4250525"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(228..>1706)
                     /locus_tag="VHV99_15725"
     CDS             complement(228..>1706)
                     /locus_tag="VHV99_15725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX3636907.1"
                     /translation="RLTGAINLLMDITERHANEVHAARLAAIVATSDDAIVSKTLEGR
                     VTSWNAAATRLFGYTPEEMIGESITRIIPPELHDEERRILAQLRRGEHIDHYETVRVA
                     KDGRRLDVSLCVSPLRDKSGHVIGASKIARDISERKQAEMLQRLLVQELNHRVKNTLA
                     TIQAIANQSMRRAKTPAEFVKGFSGRVQALARTHDLLTQTRLQGAEIMDLVRDQVLLG
                     GQDDRRVEISGPTLMLDAQAAMNLALVVHELATNARKYGALSGPDGQLSVTWETRTND
                     ERQLVLEWRESASRKIAVPRERGFGTTLIEQTLKAHGGSASIRYGADGMSGTFTLPLP
                     EQPRPRLGLAVTAPTADAAALLQRLDDKQSLAGKRVIIIEDEQLVAMDLESCLATAGC
                     EVVGSAGTVREAKTLCAEAACDAALIDVNLAGQPVDELAATLTKRNIPFAFVTGYGRE
                     ALPQGFRDALIVGKPFDETALIATVELLAYQSTSVVPLRRRQ"
BASE COUNT          259 a          581 c          551 g          315 t
ORIGIN      
        1 ctgaagcccg tagtttgtag ggtgggcaaa gcgccgaagg cgcgtgccca cgcgtttgct
       61 ctcataaaca agaaggcgcg tgcccacgcg tttgctcata aacaaatagt gttgttgtcc
      121 acgctcgaaa gacgcgtggg cacggccgcc atagcgcgtc gaagacgcgc gtaaacgcgg
      181 ttttggcggc ctttgcccac cctacgcgcg aatgcgccct acgcgcccta ctgccttcgc
      241 cgcagcggca ccacgctcgt gctctgatag gccagcaatt cgacggtggc gatgagcgcc
      301 gtctcgtcga acggctttcc cacgatcagc gcatcgcgaa aaccttgcgg cagcgcctcg
      361 cggccgtagc cggtcacgaa ggcaaacgga atattccgtt tggttagcgt ggcggcaagc
      421 tcgtcgaccg gctgaccggc gaggttcacg tcgatgagcg cggcgtcgca cgccgcctcc
      481 gcgcacaagg tcttcgcctc ccgcaccgtc ccggcgcttc ccaccacctc gcagcccgcc
      541 gtggcaaggc acgactccag atccatcgcc accagctgct cgtcctcgat gatgatcacg
      601 cgtttgccgg ccaggctttg cttgtcgtcg agccgctgca acaaggcggc cgcatccgcg
      661 gtcggcgccg tgacggcgag cccgagccgc ggccgcggct gctccggcag cggcagggtg
      721 aaggtgccgc tcatgccgtc cgcgccgtaa cggatcgagg cgctgccgcc gtgcgccttg
      781 agggtttgtt cgatcaatgt cgtgccgaaa ccgcgctcgc gcggcaccgc gatcttgcgg
      841 ctagcgcttt cccgccattc gaggacgagc tggcgctcgt cgttcgtgcg cgtctcccac
      901 gtcaccgaga gctgcccgtc aggccccgat agtgcgccgt acttgcgggc gttggtcgca
      961 agctcatgca ccaccagcgc gaggttcatc gccgcctgcg cgtcgagcat gagcgtcggg
     1021 ccgctgattt cgacgcgccg gtcgtcctgc ccgccgagca gcacctggtc gcgcaccaag
     1081 tccatgatct cggcgccttg cagccgtgtt tgcgtcagca ggtcgtgggt gcgcgccagc
     1141 gcctgcacgc gtccgctgaa acccttgacg aactcggccg gagtcttcgc gcgccgcatc
     1201 gactgattgg cgatcgcctg gatggtcgcg agcgtattct tcacgcgatg attaagctcc
     1261 tgcaccagca ggcgctggag catctcggcc tgtttgcgct cgctgatgtc gcgcgcgatt
     1321 ttcgaggccc cgatgacgtg gccgctcttg tcgcgcaacg gcgagacgca cagcgatacg
     1381 tcgaggcggc ggccgtcctt ggcgacgcgc acggtttcgt aatggtcgat gtgctcgccg
     1441 cggcgcagct gggcgaggat ccgtctctct tcgtcgtgca actcgggcgg aatgatgcgg
     1501 gtgatcgact cgccgatcat ctcctcgggc gtataaccga acaggcgcgt tgccgcggcg
     1561 ttccacgagg tcacccgtcc ctcgagcgtc ttgctgacga tggcatcgtc cgaagtcgcc
     1621 acgatcgcgg cgagccgcgc ggcgtgcacc tcgttggcgt gacgctcggt aatatccatc
     1681 agcaggttga tggcgccggt caggcg
//
LOCUS       DATASH010000580         2352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3263469,
            whole genome shotgun sequence.
ACCESSION   DATASH010000580 DATASH010000000
VERSION     DATASH010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3263469"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(298..1365)
                     /gene="glcE"
                     /locus_tag="VHV99_15730"
     CDS             complement(298..1365)
                     /gene="glcE"
                     /locus_tag="VHV99_15730"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HEX3636908.1"
                     /translation="MRIETVSMDDSERLVAEVSQAWASSTPLTIRGGNSKAFLGRPVQ
                     GTPIDTRSHRGVVSYDPTELVITARAGTPVSELNAALDAAGQMLPCEPPTFDGTATVG
                     GMVAAGLAGPRRPWSGSVRDFVLGCRVITGHARHLRFGGEVMKNVAGYDVSRLLAGSF
                     GCLGLITEVSLKVLPKPRALGSLTLDLDAHEALRELSAWRRAALPISGAAHVDGRLHV
                     RLEGGTGSVASAADRIGGAELDPQFWDALREHRLAFFDDARPLWRLSLPNASPLSSLP
                     GDTLLDWAGAQRWLKSDAPAATIRQIAHAAGGHATCFTPHVDTEPFAPLPEALLRFHQ
                     QLKQQLDPRSLFNPGRLYAGL"
     gene            complement(1365..>2352)
                     /locus_tag="VHV99_15735"
     CDS             complement(1365..>2352)
                     /locus_tag="VHV99_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX3636909.1"
                     /translation="YGLTVNNIQKLEILTIDGERITLGSDALDAPGFDYLALFTGSEG
                     MLGIVTEVTVKLLTRQQSAKVLLASFDDVEQAGAAVAQIIGAGVIPGGLEMMDNLAIR
                     AAEDFIHAGYPVDAEAILLCEVDGVESDVQEDVECVEALLRAAGATDIRLAKDEAERQ
                     RFWAGRKNAFPAVGRISPDYYCMDGTIPRRELPRVLKSIEDLSTEYGLKVANVFHAGD
                     GNMHPLILFDANAPGETERAEALGAKILELCVAVGGSITGEHGVGREKINQMCAQFNS
                     DELSFFHALKAAFDPDGLLNPGKNVPTLHRCAEFGSMHVHHGALPFPELERF"
BASE COUNT          389 a          758 c          786 g          419 t
ORIGIN      
        1 gcgcgcgaca gggttttagc ggcgtcgctg agattggttt gcatggtgtc gttcgccttc
       61 agggcttcac aggtcgatgg tcacaggccg atgggcacag accggcactg acaggtcggc
      121 actcgccagc atccattggc cagcattgac aggccggctt tgccagcgtt cacaggccgg
      181 cattgacagg tcggcgtttg ccagcgttca caggccagcg ttgacaggcc ggcgtttgcc
      241 agcactcata ggcccgcatt gacgggccgc cgttcgccag cgctcacagg tcggcattca
      301 caggccggca tagagacgac cggggttgaa gaggctgcgc ggatcgagtt gttgcttcaa
      361 ctgctgatga aaccgcagca gcgcctccgg cagcggtgcg aacggctcgg tgtccacatg
      421 tggcgtgaag caggtcgcat ggccgcccgc ggcgtgcgcg atctggcgga tcgttgccgc
      481 gggggcatcg cttttgagcc agcgttgcgc ccccgcccag tcgagcagcg tatcgccggg
      541 caaactgctc agcggcgaag cattcggcag cgagaggcgc cacaacgggc gcgcgtcgtc
      601 gaaaaaggcc aggcggtgtt cacgcagtgc atcccagaac tgcggatcga gttcggctcc
      661 gccgatgcga tcggccgccg aggccaccga tcccgtgcca ccttcgagcc gcacatgcag
      721 gcgaccgtcg acatgagccg cgccactgat tggcaacgcg gcgcggcgcc acgcggacag
      781 ttcgcgcagc gcttcgtgcg catcgagatc gagcgtgagg ctgccgagcg cgcgcggctt
      841 cggcaatacc ttcagcgaga cttcggtgat caggccgagg cagccgaagc tgccggcgag
      901 cagtcgcgag acgtcatagc ccgcgacgtt cttcatcact tcgccgccaa agcgcagatg
      961 cctggcgtga cctgtaatga cgcggcagcc gagcacgaaa tcgcgcaccg aacccgacca
     1021 cggccggcgc ggaccggcga ggccggccgc caccatcccg ccgacggtgg ccgtgccgtc
     1081 gaaggtcggc ggctcgcaag gcagcatctg accggcggcg tcgagcgcgg cattcagttc
     1141 gctgaccggc gtgccggcgc gcgcggtgat cacgagttca gtcgggtcat agctgaccac
     1201 gccgcggtgc gagcgcgtgt cgatcggcgt gccttgcacc gggcggccca gaaacgcctt
     1261 gctattgccg ccacgaatcg tcagcggcgt actgcttgcc catgcttgcg agacttcggc
     1321 gacgagccgt tcgctatcgt ccatcgatac cgtttcgatc cgcatcagaa acgctccagt
     1381 tcaggaaagg gcagtgcgcc gtggtggaca tgcatcgagc cgaattcggc gcagcggtgc
     1441 agcgtgggta cgttcttgcc ggggttcagg aggccgtccg gatcgaaggc ggccttcagt
     1501 gcgtggaaga agctcagttc gtcgctattg aattgcgcgc acatctggtt gatcttttcg
     1561 cggcccacgc cgtgttcgcc ggtgatgctg ccgcccaccg ccacacacag ttcgagaatc
     1621 ttcgcgccga gcgcttcggc gcgttccgtt tcaccgggcg cgttcgcatc gaacaggatc
     1681 agcggatgca tgttgccgtc gcccgcgtgg aagacgttgg ccactttcag gccgtattcg
     1741 gtcgagaggt cctcgatgct cttgagcacg cgcggcaatt cgcggcgcgg gatcgtgccg
     1801 tccatgcagt agtaatccgg cgagatgcgg cccaccgcgg ggaacgcgtt cttgcggccg
     1861 gcccagaagc gctggcgttc ggcttcgtct ttggcgaggc ggatatcggt ggcgcccgcc
     1921 gcgcgcaaca gcgcttcgac acactccacg tcttcctgca cgtcgctttc cacgccgtcc
     1981 acttcgcaca ggaggatcgc ttcagcatcg accggatagc ccgcatggat gaagtcttcg
     2041 gcggcccgga tcgcgaggtt gtccatcatt tcgaggccgc ccggaatcac gcccgcgccg
     2101 atgatctgcg cgaccgccgc gccggcctgc tcgacgtcgt cgaaactggc gagcaacacc
     2161 ttcgcgcttt gctgcctggt cagcagcttc acggtgactt cggtgacgat gccgagcatg
     2221 ccttccgagc cggtgaagag cgccagatag tcgaagccgg gcgcgtcgag cgcatccgag
     2281 ccgagcgtga tgcgttcgcc gtcgatggtg aggatctcga gtttctggat gttgttgacg
     2341 gtcagtccgt ac
//
LOCUS       DATASH010000581         4145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_888365,
            whole genome shotgun sequence.
ACCESSION   DATASH010000581 DATASH010000000
VERSION     DATASH010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4145
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_888365"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..232
                     /locus_tag="VHV99_15740"
     CDS             56..232
                     /locus_tag="VHV99_15740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636910.1"
                     /translation="MVQADYQFSARTDVYAQAVYQLVGGGNGNSIFNAGVYGMTQSST
                     NKQLVTAIGLRHRF"
     gene            complement(576..1364)
                     /locus_tag="VHV99_15745"
     CDS             complement(576..1364)
                     /locus_tag="VHV99_15745"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX3636911.1"
                     /translation="MYSTHRGRFDFVIPLFSNDDGRLLSIVHGDALTAYRTAAVTRIA
                     LEAFARTDARVLAVFGTGLQARAHIRAIAADSGIKRVLIVGIEHVAETVEEMQVCFPG
                     IRFEASAAQAAVQAADVIVTATRSTTPLFDGVHVKPGAFVAAIGSSKPDTRELDDTVL
                     ARAGMLVVESLEQARLEAEDLLMARPGIVDWDTVVELGHAVGSGTTQTRGFTEDITVF
                     KSLGFGLADVALAALVTRRMAGERMQRIPPGPNASPAKTVGAMA"
     gene            complement(1374..1559)
                     /locus_tag="VHV99_15750"
     CDS             complement(1374..1559)
                     /locus_tag="VHV99_15750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636912.1"
                     /translation="MHFITDDDVRRLITMRDAIEELRFALEEHGRGHARIQPRVRTRG
                     DDIMLSTMGAILPNAGV"
     gene            complement(1603..2595)
                     /locus_tag="VHV99_15755"
     CDS             complement(1603..2595)
                     /locus_tag="VHV99_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEX3636913.1"
                     /translation="MTEKANIIVIGTGGTIAGQGKAANNTSAYVCSVRGIDDILDTIP
                     HACALANLRTEQLLQNGSENFSNEHLLAIGKRVSTLLAQADVDGAVIAHGTDTIEETA
                     YFLHLTLKSSKPVVVVGSMRPPSAMSSDAALNLYDAIAVAAHPSSIGLGTLVFANDEI
                     HTARDVVKSNSFKIEAFRSPYGPLGYVIEGRPRYYRHPSRAHTLATPWSIETLGPLPQ
                     VDIVYAYGALEPEAVSAITARAQGLIYAGTGNGNVAAHLIESLRDAVRRGVHVVRASR
                     TGSGIVLRNGAQPDDTYGWLTVEDQIPSKARILLTLALTQTYDLQALQAVFERY"
     gene            complement(2653..3903)
                     /locus_tag="VHV99_15760"
     CDS             complement(2653..3903)
                     /locus_tag="VHV99_15760"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006400865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HEX3636914.1"
                     /translation="MRILILGAGVIGLSSAYYLSRAGHEVTITDRHPEVASETSSGNG
                     GQLSYSYVAPLAGPGVVSKLPRWLTRNDSPVRFRPKLSTEQWRWCFQFLSACTREQSE
                     LTTRKLLSLSFLSRTLLHELIAAEPLLDFDFVRSGKLVLHRDAGAMQSAVKLLEFQRT
                     LGCEQQALSANACVELEPTLTHLRPSLAGGIHTPSEDTADCQRFCKSLEALLRARGVQ
                     FLMNTSVDCLRPGSGGRIEAWSGGAPLASDHVVVASGAAAARLLKPVCIRVPVYPLKG
                     YSLTFDLQPQTAAPHVSITDFSRKVVYARLGKRLRIAGIADLDGYSLEPDPTRLATLK
                     AEAAAMYPDIVRSCPGSEWIGLRPATPRGTPIVGPTRHRNLWLNVGHGALGFTLAMGS
                     AALLAQWLASSNGEELRSTFALSH"
BASE COUNT          783 a         1239 c         1294 g          829 t
ORIGIN      
        1 tgcgttttag ggcggatcaa cgggaccggc ggccgacccg cattggaaca attttgtggt
       61 tcaggccgat taccagtttt cggctcgcac cgacgtgtac gcgcaagcgg tctatcagct
      121 cgtcggcggc ggcaatggca attctatatt taacgctggc gtctacggca tgacgcagtc
      181 ttcgactaac aagcagcttg tcaccgcgat cggcttgcgt caccggttct aaatgctgat
      241 ttcgagctgt acctcgtagc actccacacg acttgaagga cgccgccagg cgtctttcaa
      301 attccggtac gttcggacca gctcattgaa gggcggcaac ggggtcgact acggtctcac
      361 acgcgctacg cgacgcgtga gcttacgctt gcgggacgtc gggcagaggt cggcccataa
      421 ggcgacgctc gttccgtcgc gtcgaatgac tcaaagcagc agtattacgg cctttcgcgt
      481 catctttgtt ctgcccattc atcccgtcca catattttca aactgcgttc agctctgccg
      541 cacttgcggt tctctcagaa ccacgcgata ggatttcatg ccatcgcccc gacggttttt
      601 gccggacttg cgttcggacc tggaggtatg cgctgcatcc gctcaccggc catacggcgc
      661 gtgaccaacg cggcgagtgc tacatcggca aggccgaagc cgagcgactt gaatacagta
      721 atatcttcag taaagcctct agtttgggtt gttccgctcc caacggcgtg accaagctca
      781 accacagtgt cccaatcgac gatacctggc cgggccatca agagatcctc cgcttcgagt
      841 cgcgcctgtt ctagcgattc gacgacgagc atgccggcgc gggcgagcac cgtatcgtcg
      901 agttcgcgcg tgtccggctt gcttgacccg atcgcagcga cgaatgcgcc cggtttcacg
      961 tgcacgccat cgaagagagg cgtcgtgcta cgcgtagctg tcacgattac atctgcagcc
     1021 tgaacggctg cttgcgcggc gcttgcttcg aaacgtatgc cgggaaagca aacttgcatt
     1081 tcctcgaccg tttcagcgac gtgctcgatg ccgacgatca acacgcgctt gatcccggag
     1141 tctgcggcga tagcgcggat atgtgccctg gcctggaggc cggtgccaaa caccgcgaga
     1201 accctcgcat cggttcgcgc gaacgcttcg agtgcaatac gcgtgactgc tgcggtacgg
     1261 tacgcggtga gcgcatcgcc gtggacgatt gacaggagac gtccatcgtc attcgagaag
     1321 agcgggatca cgaaatcgaa cctgcctctg tgagtcgaat acactttcgc gcctcacacg
     1381 cccgcgttcg gcagaatcgc gcccatcgtg ctgagcatga tgtcgtcgcc cctggttctg
     1441 acgcgtggct gaatccgtgc gtggcccctg ccatgttctt ccagcgcgaa acgcagttcc
     1501 tcaatcgcat cgcgcatcgt gatgaggcgg cgcacgtcgt cgtcggtaat gaagtgcatg
     1561 atgtgtgctt ctggtggggg cggttgtaaa ggagcgcgcg cttcagtagc gctcgaagac
     1621 agcttgcaac gcttgcaagt cgtaagtttg tgtcagtgcg agcgtcagaa ggatgcgggc
     1681 ttttgaggga atctgatctt cgacggtgag ccagccgtat gtgtcgtcgg gttgcgcgcc
     1741 gttacgcaac acgatgccgc tgccggtgcg cgatgcgcgc acgacatgaa cgcctcgccg
     1801 gacagcatcg cgcagcgact cgatgagatg cgcagcgacg ttgccgttgc cggtgcccgc
     1861 atagatcaac ccttgcgcgc gggcggtgat cgcgctcact gcttccggtt caagcgcacc
     1921 gtaggcgtag acgatgtcga cctggggcaa tggcccgagc gtctcaatcg accacggtgt
     1981 cgcaagcgta tgggcacgcg atggatgacg gtagtagcgt ggccgccctt cgatcacgta
     2041 accgagcggg ccgtacggtg agcggaacgc ctcgatcttg aagctgttgc tcttcacaac
     2101 gtctcgtgcg gtgtggattt cgtcgttggc gaacacgagt gttccgaggc caatcgacga
     2161 cgggtgtgcg gcgaccgcga tcgcatcgta gaggttcagc gccgcgtcgg aactcatcgc
     2221 cgacggcgga cgcatcgagc caaccacgac caccggcttg gaactcttga gcgtgagatg
     2281 aaggaagtac gcggtctcct cgatcgtgtc ggttccgtgc gcaatcacgg cgccatcgac
     2341 gtcggcttgc gcgagcagtg tggacacgcg cttaccgatc gcgagcagat gctcgttact
     2401 gaaattctcg gagccgtttt gcagcaactg ctccgtgcgc agattcgcca gcgcgcaggc
     2461 atggggaatc gtatcgagaa tgtcatcgat gcctcgcacc gaacagacgt atgcggacgt
     2521 gttgttggcg gctttgccct gaccggcaat cgtgccacct gtgccgataa cgatgatgtt
     2581 ggccttttct gtcatgcgat gtcgatgatg ttgggggagt gagcacgtca cgaagcgtat
     2641 cgccgcgcgg gttcaatgcg aaagagcaaa tgtgctccgt agttcctcgc cgttgctgct
     2701 tgccagccac tgcgccagca gtgcggcaga acccatggca agcgtgaagc cgagcgcgcc
     2761 atggcctacg ttgagccaca gattgcggtg gcgtgtcggt ccgacgatcg gcgtgccacg
     2821 cggcgtcgcg gggcgcaacc cgatccattc actacccgga caggatctga cgatgtcggg
     2881 atacattgca gccgcctctg ctttcagcgt cgcaaggcgt gtgggatcgg gctcgagcga
     2941 ataaccgtcg agatcggcga tgcctgcgat tcgcagccgc ttgccgagcc gcgcatacac
     3001 tactttgcgg ctgaagtcgg tgatgcttac gtggggcgca gccgtctgcg gttgcaggtc
     3061 gaacgtcagg ctgtagcctt tgagcggata gaccggcaca cggatacaga caggcttcag
     3121 taaccgggcc gcagccgcgc ctgaggcaac gacgacatga tcggatgcga gcggcgcacc
     3181 accgctccag gcttcgatgc ggccgcccga tccaggcctc agacaatcga ccgatgtgtt
     3241 catcaggaac tgcacaccgc gagcgcgcag cagcgcttcg aggctcttgc agaagcgttg
     3301 gcaatcggcc gtgtcctcgc tcggcgtatg gatgccgccc gcgagagaag gccgcagatg
     3361 cgtgagcgtg ggttcgagtt cgacgcacgc attggcgctc aatgcctgct gttcacagcc
     3421 gagcgtacgc tggaattcaa gcagtttgac cgcgctctgc atcgcacccg cgtcacgatg
     3481 cagcacgagc ttgccggaac ggacgaaatc gaagtcgagt aagggttcgg cggcgatcag
     3541 ttcgtgaagc agcgtccggc tcaggaacga cagtgacagc agcttgcgtg tcgtgagttc
     3601 gctctgttcg cgcgtgcatg ccgacagaaa ctgaaagcac cagcgccact gttcggtgct
     3661 cagcttcggg cgaaagcgca ccggcgaatc gttgcgcgtg agccagcgcg gcagcttcga
     3721 gaccacacca gggccggcta gcggcgcaac atagctatac gataactggc cgccgttgcc
     3781 ggagctggtc tcgctcgcca cctccggatg ccggtccgtg atcgtgactt cgtggccggc
     3841 ccggctcagg taatacgcgc tcgacaaacc aatgacgcct gcaccgagga tcaatatccg
     3901 catactgccg tacccgcttt tcttcgtgtg tttgcatgga ggccgtttgc cgcagacgcg
     3961 tcatcagccc gtcgatcgga ctctacgggc ccgccgacgt gccggattgc atcgatctgc
     4021 cggaaagtag aagaatcgcg acaggcacgt gcgaaacatg acggtcggcg ttgagggtcg
     4081 gcgttgagcc aacccgatgg atcatgctgc ggcagcgccc cgcgatgtgc gcagcttgcc
     4141 gggtg
//
LOCUS       DATASH010000582         6144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3263647,
            whole genome shotgun sequence.
ACCESSION   DATASH010000582 DATASH010000000
VERSION     DATASH010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6144
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3263647"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VHV99_15765"
     CDS             <1..345
                     /locus_tag="VHV99_15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636915.1"
                     /translation="RMLAARGIDADNGPGFALDRRVPIVLGGSPDAPSNLDLLPWAGQ
                     QGERRKARAAVMLKRCVCEGKLSLAEAQATIVGNWSVVYSGFGQMSCDVRRLDVATGE
                     SNARSDGRGSAP"
     gene            448..798
                     /locus_tag="VHV99_15770"
     CDS             448..798
                     /locus_tag="VHV99_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636916.1"
                     /translation="MIHSRLAALALTFGCLFGSAAALAAEPVRCGSASSLQIRGDVPA
                     AISFDVYRQLRPLNVQRVALLQAAGEVKRLPDGLAVCEVADDGVNDPSAVLVHVPQGK
                     NAWWVSAANVQAAD"
     gene            1228..1497
                     /locus_tag="VHV99_15775"
     CDS             1228..1497
                     /locus_tag="VHV99_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX3636917.1"
                     /translation="MNLIVRNIAESCSEQEVRDFLKHELGHYAKNIEVVDAGKPGAYA
                     TVELDADVPYVGDVIARQIHGKQLGGLTLEASADLFSDDESPPSH"
     gene            complement(1623..2483)
                     /gene="nadE"
                     /locus_tag="VHV99_15780"
     CDS             complement(1623..2483)
                     /gene="nadE"
                     /locus_tag="VHV99_15780"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEX3636918.1"
                     /translation="MTQPDSAERQASIAAEMHVAAEFDAAEEIERRVAFLANYLRTNS
                     LKTFVLGISGGVDSTTAGRLAQLAVERLRAEHYDARFVAIRLPYGVQKDEADAQQALS
                     FIRADENLTVDIKPAADAMLAALNNSNVRFNDDSQQDFVHGNIKARQRMIAQYAVASA
                     RAGVVIGTDHAAESVMGFFTKFGDGGADVLPLTGLNKRRVRALSKALGAPETLAHKVP
                     TADLEMLRPQRPDEDAYGIPYDDIDDFLEGKPVSDAARETILRFYDVTRHKRALPYTP
                     FDWPVKSGAQ"
     gene            complement(3082..3954)
                     /locus_tag="VHV99_15785"
     CDS             complement(3082..3954)
                     /locus_tag="VHV99_15785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX3636919.1"
                     /translation="MLASSIAAVFVVLWSTGFVVARAITPYADPNLFLLARFAGTALI
                     FALAAFAARAMWPTGRDLGKHLLAGALLQGVYLGAGYWAVAQGLSAGVMALLGALQPL
                     ATAAVAAPLFGERLSRRGWTGMALGLAGVALVLGPKLAAAAPPTQHGHAPPWLVVFIS
                     ILAIGAITAGTLFQKTSLAKADIRSASAVQNFGAAVVAAILALALGEHRWIASAALWA
                     SLAWGIVMLSGISVTLLVWMVRRGDAARATALMFLAPPLAALEGYVGFGETLLPVQLA
                     GFAVALVGVLLARS"
     gene            complement(4170..5729)
                     /locus_tag="VHV99_15790"
     CDS             complement(4170..5729)
                     /locus_tag="VHV99_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEX3636920.1"
                     /translation="MPPLFRRLLLLFHALRYGARLIWLAAPADHKLHWLIELVGRVHA
                     SGRSGERLHNVLPALGPLASRFAQTLVERPELATGTLHDAIDAIDHLEAPLPSQASEQ
                     ALARAFGRPLATLFSAVDLVPVRSGFAEQTHLARLLVPVNGHSEVAIKLVRADQLQQI
                     GDELALLRWVARWLEKLSGSARRLQLRRLAQTFTDDILRRFDLRVEAANLSQTGHHFD
                     GDTRIVVPDVIWDLCTNHTLTTQRVSTLPASDLPGLHAHHVKLAPLAAHIVEVVTEQA
                     FEHGFFHATLDARRVRVSVEPDTLGRLVLAEFSIMSSLSTGEREFFVHGATALFDQDY
                     GRLAELHREAGHVPHDTRKEMLEAELRTRAEAHFAAEPEDRSAGSLFHHLLHAVHPFD
                     GAVPGRLATAQRSFQQAEMLARALHPGVDTWTVTRRVLAGIARRDMDHRGWIKRISHE
                     LPHLAHMLPRVPQLAVRYLQHEHDRARTPRQNAELMLDIGREYRRTRVLLWACAVCGG
                     VLGAGTVLLMW"
     gene            5910..>6144
                     /locus_tag="VHV99_15795"
     CDS             5910..>6144
                     /locus_tag="VHV99_15795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011546920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HEX3636921.1"
                     /translation="MLAEQRHQYILAQVAKTGALSVAELVRELNVSRETIRRDLNALA
                     GRGLLVTTHGGALSSDRREPDLDTREAANAGAKR"
BASE COUNT         1117 a         1923 c         2001 g         1103 t
ORIGIN      
        1 cgcatgcttg ccgctcgcgg catcgacgcc gacaacggtc cgggtttcgc gctggaccgg
       61 cgtgtgccga ttgtgctcgg tggctcgccg gatgcaccct ccaatctcga tcttctgccg
      121 tgggcaggcc agcagggcga gcgccgcaag gcgcgcgccg cggtcatgct caaacgctgc
      181 gtttgcgagg ggaaactgag cctcgcggag gcgcaagcca cgattgtcgg taactggtct
      241 gtggtctatt cgggcttcgg ccagatgtct tgcgacgtgc gccgtctcga tgtcgcgacc
      301 ggcgagagca acgcgcgaag cgacggtcgc ggcagcgcgc catgagaccg cctgtttcaa
      361 tctgcgttga tctggcggtt cgttcgcgtg cttccccgcc gcgccgcgag cagcgcgctg
      421 tcccgacttg tttgtcggag aatcaccatg atccattcac gccttgccgc gctggcactc
      481 actttcggct gtctgttcgg tagcgccgcg gcgcttgccg ccgaacccgt gcgctgcggc
      541 agcgccagct cgctgcagat tcgcggcgat gtgcccgcag caatcagttt cgacgtgtac
      601 cggcaactgc ggccgttgaa cgtgcagcgg gtcgcgcttc tgcaggcagc cggggaggtg
      661 aaacgcttgc cggacgggct cgcggtatgc gaagttgccg acgacggcgt caacgatccg
      721 tccgccgtgc tggttcatgt gccgcaaggc aagaatgcat ggtgggtaag cgccgccaac
      781 gtgcaagcgg cggactgatc ccgacggtca ggccgccttt gcgcacgccc ccgcagaatg
      841 ccggtggcaa tgcttagcgc ggccgttttt tgcgtccagc gtctgcgtct ggcgcctgtg
      901 ccgggcctgc atcactgaat gaacatgtgt tttgctattt ttactgctgt ttattcaggt
      961 acagtgcact aacggaagat tttaagatcc gtcttttacg gcattgtgtc gcatcgcttt
     1021 tgttgtcgat tgtggctatc ctgaaaacag gacgaaattc gaaggggaga tttaaatgat
     1081 tgccttctat ctgtcattgg ctgccttggg tgtggctggc gctgcattga tccgccaact
     1141 cgcagccgct caggagaaac agcgtgccgc actgcgcccg gtgcgcgtgc atgcctcaga
     1201 tcgcagcact cgccgagggt aggccgcatg aacctgatcg tgcgcaatat cgcggaatcg
     1261 tgcagcgaac aggaagtgcg ggattttctc aagcatgagc ttgggcatta cgcgaaaaat
     1321 atcgaggtgg ttgatgccgg gaagcctggc gcctacgcca cggtcgagtt ggacgcggac
     1381 gtgccctatg tgggcgacgt gatcgcgcgt cagatccacg gcaagcaatt gggcggcttg
     1441 acgctcgagg cgagcgccga cttatttagc gacgacgagt cgccgccttc tcactaagct
     1501 tgtcgcaact gcgcgtggca aagcgccgag ttgccgagcg cggcaaatgg gacgagcgcg
     1561 cggtgtttgc gcattcctga cgcgcatcgt ctccccgcgt tctcgttatt gcacgccttc
     1621 tctcattgcg cacctgattt caccggccaa tcgaacggcg tgtagggcag tgcccgcttg
     1681 tgccgcgtga catcgtagaa gcgcaagatc gtttcgcgcg cggcgtcgct caccggtttg
     1741 ccttccagaa agtcgtcgat gtcgtcgtaa gggatgccgt atgcgtcttc gtccggccgt
     1801 tggggacgca acatttcgag gtcggcggtc ggcactttgt gcgccagcgt ctcgggagcg
     1861 cccaaggctt tggacagtgc ccgcacccgg cgcttgttca ggccggtgag cggcagcaca
     1921 tcggcgccgc cgtcgccgaa cttcgtgaaa aatcccatca ccgattcggc cgcgtggtcc
     1981 gtaccgatca ccacgccggc ccgcgcactg gcgaccgcgt attgcgcaat cattcgctgc
     2041 cgtgccttga tattgccgtg gacgaaatcc tgctgtgagt cgtcgttgaa gcgcacgttg
     2101 ctgttattca gcgcggcaag catcgcatcg gcagcgggtt tgatatcgac ggtgagattt
     2161 tcgtccgcac gaatgaaact caaggcctgc tgcgcgtcgg cttcatcttt ttgcacgccg
     2221 tagggcaaac ggatcgcgac gaagcgcgcg tcgtaatgtt cggcgcgcag ccgttcgacc
     2281 gcgagttgcg caagccgccc ggcggtggtc gaatcgacgc cgccgctaat gccgagcacg
     2341 aaggttttca ggctgttggt gcgcaggtag ttcgccagaa aagccaccct gcgctcgatt
     2401 tcctctgcgg cgtcgaactc ggcggcgacg tgcatttccg ctgcgatgct tgcctggcgt
     2461 tcggctgagt ctggttgtgt catgttcctc ttgcctccgt gtgcggcggt gcgcctgtcg
     2521 acgtttcaac gttcgcgcgg cttgcaggag cggcggcgcc acgaacgcgc ccgttctgcg
     2581 ggcatgatgt gacgaatcaa tatcgcggtt ttatcgcggg ataatggctc taaattcatt
     2641 ttgcgcgact tttaaacagc tgcgcggaac gttaaatatg gactgtcaat gctgttaggg
     2701 gtgaatggcc gattctttca tcggcagatg ccaatgtgtt tagcaatatg cccgttccga
     2761 gcgctgcatg agccggcgag cagtcaatag taatcaggtt tgctcaaata cgcgatgaac
     2821 tacgccaatc ggccaggcca gcctgcaccc ggttgcgtga tccgcgactg aacaggcggg
     2881 cgagagtgcg gagcgagtat caagccgata acgttcgcga gacccgggat gtccgctagc
     2941 ggacaggctc gtagcgtaag ctgctacggt tcgagcaatc cgcagcagcg ttcagccgcg
     3001 gttgacgagc aaaaagtacc gcccggcacg catgacgaac gcgcattgat cgacgcagac
     3061 cggtgtgcac cattcaaact ctcacgacct cgccagcagc acgcccacca gcgccacggc
     3121 aaatccagcg agctgcaccg gcaacagcgt ttcgccgaag ccgacatacc cttcgagcgc
     3181 ggcgagcggc ggcgcgagaa acatcaacgc ggtagcacgc gcagcatcgc cgcgcctgac
     3241 catccacacc agcaaggtga cgctgatccc ggacagcatc acgatgcccc acgccagcga
     3301 cgcccacaaa gcggccgatg caatccaacg atgttcgccg agcgccagcg caagaattgc
     3361 cgcaaccacc gccgcgccga aattctgcac ggcacttgcg ctgcggatat cggccttggc
     3421 cagtgaagtt ttctggaaca acgtgcctgc ggtgattgcg ccgatcgcaa ggatcgagat
     3481 gaacacgacc agccacggcg gcgcgtggcc gtgttgggtg ggcggtgccg ccgcggcgag
     3541 ctttggtccg agaaccagcg cgacgcctgc caggccgagt gccatgccgg tccagccgcg
     3601 ccgcgataga cgctcgccaa acagcggcgc ggcgacggcg gcagtcgcga gcggctgcaa
     3661 cgcgccgagc aaggccatca cgccggcgct caatccctgg gcgacggccc agtagcccgc
     3721 gccgagatag acgccttgca gtagcgcgcc cgcgagcaga tgtttgccga gatcgcggcc
     3781 ggtcggccac atggcgcgag cggcgaacgc ggcaagcgcg aatatcagcg cggtgccggc
     3841 aaagcgcgcg agcaggaaca gattgggatc ggcgtagggt gtgatcgccc gggcgaccac
     3901 gaaaccggtc gaccacaaca cgacgaacac ggcggcgatt gacgaagcga gcatcggagg
     3961 cggggggagg gaaaagccgc catcttgccg ggacacgcga cgcgcgtctt gttcgaagct
     4021 gcaatcgcgg gtgttttgcg caatggcgga ttgcctgagt cgcgccgcgg cgcgtcctat
     4081 ccttatcagc tagcagggcg gcggcatcag cgtcccgccg gcatcgccac atcagcggca
     4141 tcagcctccc gccggcatcg ccaccaactt caccacatca ataaaacggt ccccgcgccg
     4201 agcactccgc cgcacaccgc acatgcccat agcaacacac gcgtgcgccg atattcacgg
     4261 ccgatgtcca gcatcaactc tgcattctgc cgcggcgtgc gggcgcgatc gtgctcatgc
     4321 tgcaagtagc gcacggccag ttgcggcacg cgcggcagca tatgcgccaa atgcggcagc
     4381 tcatgcgaga tgcgtttgat ccagccgcgg tgatccatgt cgcgccgtgc aatccccgcc
     4441 agcacgcgtc gtgtgacagt ccacgtatcg acgcccggat gcagcgcgcg ggccagcatt
     4501 tccgcctgct ggaacgagcg ctgcgcggtg gcgagacggc cgggcacggc gccgtcgaac
     4561 ggatgcaccg cgtgcagcag atgatgaaat agcgaacccg ccgagcggtc ttccggctcg
     4621 gccgcgaaat gcgcttcggc gcgtgtgcgc aattcggctt cgagcatttc cttacgcgtg
     4681 tcgtgcggca cgtgccccgc ttcacgatga agttcggcaa gccggccgta gtcctggtcg
     4741 aacagcgcgg tcgcgccatg cacgaagaac tcgcgttcgc cggtcgacag actcgacatg
     4801 atcgaaaact ccgccagcac gaggcgtccc agcgtgtccg gttcgacgct tacgcgcacc
     4861 cggcgtgcat cgagcgtcgc gtgaaagaag ccgtgctcga acgcctgttc ggtcaccacc
     4921 tcgacgatat gcgcggccag cggcgcgagc ttgacgtgat gggcgtgcag gccgggcagg
     4981 tcgctggcgg gcaaggtgct gacgcgctgc gtcgtcagcg tgtggttggt gcataggtcc
     5041 cagattacgt ccggtacgac gatgcgcgta tcgccgtcga aatgatggcc ggtctggctt
     5101 agattggcgg cttcgacgcg cagatcgaag cggcgcagga tgtcgtcggt gaaggtttgc
     5161 gccaacctac gcagttgcaa ccggcgggcg gagccggaaa gcttctccaa ccagcgcgcg
     5221 acccagcgca gaagcgcgag ttcgtcgccg atctgttgca actgatcggc gcgcaccagc
     5281 ttgatggcca cttcgctatg gccgttgacc ggcacgagga gccgcgcgag atgcgtctgt
     5341 tcggcaaagc cgctgcgcac cggcaccaga tcgactgcgc tgaagagggt ggcgagcggc
     5401 cggccgaatg cacgcgccaa tgcctgttcg gatgcctgcg acggcagggg ggcttcgaga
     5461 tgatcgatgg cgtcgatggc atcgtgcagg gtgccggtcg cgagttcggg ccgttcaacc
     5521 agggtctgcg cgaagcggct cgcgagtggt ccaagcgcgg gcagcacatt gtgcaggcgc
     5581 tcgccgctgc gcccggaagc gtgcacgcgg ccgaccagct cgatcagcca gtgcagtttg
     5641 tggtcggccg gggcggcaag ccagatcagg cgcgcgccat aacgcaacgc gtggaacaac
     5701 agcagtaaac ggcgaaacag tggcggcatc ggcagttcgc ttgacggacc acggaccgca
     5761 cggcatcgac aggcgtcggc gcgcggccag ggcgttcagg ttagcaggta agcgcgtccg
     5821 cgggagcatc cccgcgccgc gcgtaataca atacgccggc ctttcaccca tggcgaaacc
     5881 caagcgtgcc ggcttccggc cctcaacaga tgctcgcaga acaacgtcac caatatatcc
     5941 tggcgcaagt cgccaaaacc ggcgcgcttt cggtcgctga actggtgcgc gaactgaatg
     6001 tgtcgcgcga gacgatccgc cgtgatctga atgcgctcgc cgggcgcggt ctgctcgtca
     6061 cgacccacgg cggcgcgctg tcgagcgacc ggcgcgagcc cgatctcgat acgcgcgaag
     6121 ccgccaacgc tggcgcgaag cgag
//
LOCUS       DATASH010000583         1737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6821493,
            whole genome shotgun sequence.
ACCESSION   DATASH010000583 DATASH010000000
VERSION     DATASH010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6821493"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="VHV99_15800"
     CDS             <1..680
                     /locus_tag="VHV99_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013699775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX3636922.1"
                     /translation="LTLAPNATSQLCYVISGTGSVTQGETRFPFGQGDFFTMPGGDAA
                     VLSADATATLYYVNDAPLLTYLGTTGVEARFAPTLYPAARAQAELRKVAEEANAGLRN
                     RISVLMGNTNFPQTRTVTHVLWAMFGIVPPNSIQKPHRHQSIALDFIAGGQPGVYTLV
                     GTEVDENGNILNPTRVDWESGMAFVTPPGYWHAHFNETNENAYVIPIQDAGLQTWLRT
                     LDIRFAR"
     gene            complement(677..1630)
                     /locus_tag="VHV99_15805"
     CDS             complement(677..1630)
                     /locus_tag="VHV99_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013343418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3636923.1"
                     /translation="MDYFAAVRAFVCAAELQSFSKTAREMAVKTSTVSRYVSELEKDL
                     GIALFNRSTRGLVLTEGGRLFREHAMVALQALDDARQVTSSLNRTPQGVLRVTMPAAF
                     GRRHIVPHLPAFMARYPAIGLDIVSTDETLNMIEAGIDVAIRIGVLPDSSLMARRIAP
                     HRRIVCGSPAYFERSGMPRAPEDLAAHDALRLSLLPDDKWSFTHAADSHAASEQVVVE
                     LQGRLRADDSEAVLELAVAGCGLALLPTWLAATALREGRLQHVLPEWEARTGRAEPAV
                     WAVYPPKKIVSSKVRAFVDFYAEVFGEPPYWDDGLALARQP"
BASE COUNT          333 a          574 c          528 g          302 t
ORIGIN      
        1 cgttgacgct cgccccgaac gcaacctcgc agctttgcta cgtcatcagc gggaccggca
       61 gcgtgacgca gggcgagacc cgcttcccgt ttggtcaggg cgatttcttc acgatgccag
      121 gcggcgatgc cgccgtgctg tccgccgacg cgacggcaac gctctattac gtcaacgacg
      181 cgccgctgct cacctatctc ggcacgaccg gcgtcgaggc gcgctttgca ccgacgctct
      241 atccggctgc ccgggcccag gcggaattgc gcaaggtcgc ggaagaagcc aatgcgggcc
      301 tccgcaaccg catcagtgtg ctgatgggca ataccaactt cccccaaacg cgaacggtca
      361 cgcatgtttt gtgggccatg ttcggcattg tgccgcccaa ctcgatccag aagccgcacc
      421 gccaccagtc gatcgcactc gacttcatcg ccgggggcca gccgggtgtc tatacgctgg
      481 tcggtacgga ggtggacgag aacggcaata tcttgaatcc gacccgcgtc gattgggaat
      541 ccgggatggc gttcgttaca ccgccgggat actggcatgc gcacttcaat gaaacgaacg
      601 agaacgctta tgtgattcct atccaggatg ccggcttgca gacctggctg cgcacgctcg
      661 atatccgctt cgcccgttaa ggctgtcgcg ccagcgcgag tccgtcgtcc cagtacggcg
      721 gctcgccaaa cacctcggcg taaaaatcca cgaatgcgcg caccttcgac gatacgattt
      781 tcttcggggg atagaccgcc cagaccgctg gttcggctcg ccctgtgcgc gcctcccatt
      841 caggcagcac gtgctgcagg cgcccttcgc gcagagccgt agccgcgagc catgtcggca
      901 gaagagcaag cccgcagccg gccacagcga gttccagcac ggcttcagag tcgtcggcgc
      961 gcaagcgccc ttgcaattcc accaccacct gctccgacgc cgcgtgactg tctgcggcgt
     1021 gggtgaaaga ccatttgtcg tccggcagca gggacaagcg aagcgcatcg tgcgcggcca
     1081 gatcttcagg ggcacgaggc atcccgctgc gctcgaaata cgccgggctg ccgcagacga
     1141 tgcgacgatg cggtgcaatg cgcctcgcca tcaatgacga atcgggcagc acgccgatac
     1201 ggatcgccac gtcgatccca gcttcgatca tattgagcgt ttcgtcggtc gacacgatat
     1261 cgaggccgat cgcggggtag cgcgccatga acgccggcag atgcggcacg atatgccggc
     1321 gcccgaatgc cgccggcatg gtgacgcgta gcacgccttg cggtgtgcga ttcagtgacg
     1381 acgtcacctg gcgcgcatca tcgagcgctt gcagggcaac catggcatgt tcacgaaata
     1441 gcctgccgcc ctccgtcagg acgagcccac gcgtcgaccg gttaaacagg gcaatgccca
     1501 ggtccttttc cagctcgctc acgtagcgcg agaccgtcga ggtcttgacc gccatttcac
     1561 gggccgtctt gctgaagctc tgcagttcgg ctgcgcaaac gaaggccctt acggccgcaa
     1621 aataatccat ggtatttccg acgcaaagcg cgcgtcctgc aagaaaactt cacgaagtgg
     1681 ggaccggaac ccgcgcaacg ctctgcgtgg gaggcgttgg cgcatgcgcg tggaccg
//
LOCUS       DATASH010000584         3397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_96426,
            whole genome shotgun sequence.
ACCESSION   DATASH010000584 DATASH010000000
VERSION     DATASH010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96426"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..3397
                     /gene="putA"
                     /locus_tag="VHV99_15810"
                     /pseudo
     CDS             137..3397
                     /gene="putA"
                     /locus_tag="VHV99_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012212480.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
BASE COUNT          620 a         1098 c         1119 g          560 t
ORIGIN      
        1 cgacgcgacg agcggcggca atgcttccgg ctcgggcaat cgataggccg cggtgatccg
       61 cgcgcgcaac ggcgaagggg gcgccagtgc gtcgtcgaat gcgggcaggc cgggagagga
      121 agcggtcggg ctcgccgaag gcgacgacga aacggcgtct cacccatttc tcgaattcgc
      181 gcagagcgtg cagccgcagt cggtgctgcg cgccgcgatc acggccgcgt accggcggcc
      241 ggagccggag tgcgtgccgt tcctgctcgg tcaggcgcgc ttgccggcga gtctcgcggg
      301 cgacgtgcag gcaatggccg gcaagctggt cgaaacgctg cgcaccaaga gcacgggtgg
      361 tggcgtcgaa ggcttgatcc acgaattctc gctgtcgagc caggaaggcg tcgcgctgat
      421 gtgcctcgcc gaagcgctgc tgcgcattcc cgatcgcgcc acgcgcgacg cgctgatccg
      481 cgacaagatc agcaagggcg actggaaatc gcatgtgggc caggcgcctt cgctgttcgt
      541 caacgccgcg acttggggcc tgatgatcac cggcaagctg gtgacgacca atagtgaaac
      601 gggtctgtcg tcggcgctca cgcgtctgat cggcaagggc ggcgaaccac tgattcgcaa
      661 gggcgtcgat atggcgatgc gcctgatggg cgaacagttc gtcaccggcg agaacatctc
      721 cgaagcactg gccaatagcc gcaagtacga agcacgcggt ttccgctact cgtacgacat
      781 gctcggcgaa gcagccacca ccgaagccga cgcgcagcgc tactacgcgt cgtacgaaca
      841 ggcgatccac gcgatcggca aggccgccgg cggccgcggc atttacgaag gcccgggcat
      901 ctcgatcaag ctctcggcac tgcacgcccg ctactcgcgt tcgcagcagg aacgcacgat
      961 gagcgaattg ctgccgcggg tgcgctcttt ggcgatcctc gcgcgccgct acgacatcgg
     1021 cctgaacatc gacgctgaag aagccgaccg cctcgaaatc tcgctcgatc tgctcgaagc
     1081 gctgtgtttc gatccagaac tggccggctg gaacggcatc ggttttgtgg tgcaggcgta
     1141 ccagaagcgc tgcccgttcg tgatcgagta tctgatcgat ctggcacgcc gcagccgtca
     1201 ccgcatcatg gtccgtcttg tgaagggcgc gtactgggat accgaaatca agcgtgcgca
     1261 agtggacggt ctcgaaggct atccggtcta cacgcgcaag atctacacgg acgtgtccta
     1321 cctcgcttgc gcgaagaagc tgctgagtgc gccggacgcg gtgtatccgc agttcgccac
     1381 gcacaatgcg cacacgctgt cggcgatcta tcacctcgct ggccataact actaccccgg
     1441 gcagtacgag ttccagtgcc tgcacggcat gggcgaaccg ctgtacgagg aagtcactgg
     1501 ccgtgacaag ctgaaccgtc cgtgccgcgt gtacgcgccg gtcggcacgc atgaaacgct
     1561 gctcgcgtac ctcgtgcgcc gcctgctgga aaacggcgcg aacacgtcgt tcgtgaaccg
     1621 catcgccgac gaaaacgtcg cgatcaagga cctgatcgcc gacccggtcg atgaagcctc
     1681 caagatcgtc ccgctcggcg cgccgcacgc gaagattccg ctgccgcgca atctgtttgg
     1741 cgccgagcgt ctcaattcga tgggcctcga tctgtcgaac gagcatcggc tggcatcgtt
     1801 gtcgtcggcg ttgctcgcga gcgcgcatca tccgtggcgc gccgcgccga tgctcgaaga
     1861 caacgagatc gccgtgggcg tcgcacgtga cgtgcgtaac ccggccgatc atcgcgacct
     1921 cgtcgggacg gtcgtcgaag cgacgccgga acacgtgagc gcagcattgg cacacgcggt
     1981 cgccgtcgca ccgatctggc aagccacgcc ggtcgacgcc cgcgcggact gtctcgcccg
     2041 tgccgccgat ctgctcgaag cgcagatgca cacgctgatg gggctggtgg tgcgtgaagc
     2101 cggcaagtcg ctggccaacg cagtcgcgga aatccgcgaa gcgatcgatt tcctgcgcta
     2161 ctactcgacg cagatccgca acgagttctc caacgacacg caccgcccgc tgggcccggt
     2221 ggtctgtatc agcccgtgga acttcccgtt ggcgattttc atgggccagg tggccgccgc
     2281 gctcgcagcc ggcaacacgg tgctcgcgaa gccggccgaa cagacaccgc tgatcgccgc
     2341 ccaagccgtg cgtatcctgc gcgaagccgg cgtgccggcg ggcgcggtac aactgctgcc
     2401 gggcgacggc gaaacggtcg gcgccgcttt ggtggccgac gcgcgcactc gcgcggtgat
     2461 gttcaccggc tcaacggaag tcgcgcggtt gatcaacagg acgctgtcga accgcctcga
     2521 ccccgacggc aagccgattc cgctgatcgc cgaaaccggc ggacagaacg cgatgatcgt
     2581 cgactcgtcg gcgctcgctg agcaggtagt cgcggacgtg ttgcaatcct cgttcgattc
     2641 cgccggtcaa cggtgttccg cgttacgcgt tctctgtctg caggacgatg tcgcggaccg
     2701 cacgctggaa atgctgacgg gcgcgatgcg cgaactggca gtgggcaatc cggatcgcct
     2761 gtcgatcgac gtcggcccgg tgatcgacct cgacgcgaag cgcggcatcg acgcgcacgt
     2821 cgcggcgatg cgcgagaaag gccgcaaggt cgagcaacta ccgatgccgg aaggcggcgc
     2881 acaaggcacc tttgttccgc cgacgctgat cgaactcgac agcatcgacg aattgaagcg
     2941 cgaagtgttc ggcccggtgc tgcatgtggt gcgttaccgc cgcagccagc ttgacaaact
     3001 actcgaacag atccgcacca ccggctacgg cctgacgctt ggcatccata cgcggatcga
     3061 cgagaccatc gcccacgtga tcagccgagc gcatgtcggc aacatctacg tgaaccgcaa
     3121 cgtgatcggc gcggtggtgg gtgttcagcc gttcggcggc gaaggtttgt ccggcacggg
     3181 tccgaaagcc ggcggcgcgc tgtatctgca gcgtctgctg gctacgcgtc cggctggttt
     3241 gccgaagtcg ctggcgcaga cgttgatcgt ggatgcgccg caagcgtcgg aaaacagcga
     3301 caatccgtcc gctgcattga cggcttaccg cgactggctg atcggcgaac agcaaccggt
     3361 gttggccgca cgtgtcgacg gtcatctgtc gcatgtg
//
LOCUS       DATASH010000585         7163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1481523,
            whole genome shotgun sequence.
ACCESSION   DATASH010000585 DATASH010000000
VERSION     DATASH010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7163
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1481523"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1342)
                     /locus_tag="VHV99_15815"
     CDS             complement(<1..1342)
                     /locus_tag="VHV99_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011546425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX3636924.1"
                     /translation="MSKRLYRVYRAAGLALAATALVACSTASAVKPIPLSQLTNDDQT
                     FVQLREAARNNDAARAAQLASMIPNYPAPSYLEYFQIKPQLFDSSGHARIDAPDAPVL
                     AFLQKYDGQAIADRLRNDYLTVLGARHDWRNFDQQYARFVLNDDTQVKCYALESRAAR
                     GENVADAARALLVDPKVYGDGCVDLITSLAVNQQFSADDVWQQIRLAYEQNYTNTGSK
                     LVDALSNQPPDPVLFGQATSTPPLLLARGVGPDTQSHQLALLAITRMARNDPAMAAAT
                     FASVAPSLSSPERAIGWGTIAYQAAAKQMPGAVDWYRLSANAPLSNPAYEWRTRSALL
                     AGDWTMVRWSIEQMPAALRNQPSWVYWHARALKQAGDTTTANQEFESISQGFNFYGQL
                     AAEELGQKITVPPKTTVSDAEVQQAGSTPGFDLAQRFYALNLRLEGNREWNWPLR"
     gene            1420..1959
                     /locus_tag="VHV99_15820"
     CDS             1420..1959
                     /locus_tag="VHV99_15820"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEX3636925.1"
                     /translation="MLLETRLQAASEPAHNAALSRRMLDALKHYGVRRVGFYWPLAGE
                     FDARATIAIWLAADSQREASLPIVKDRGVPLEFHAWAPDTPMKIGHHKIAEPTSGRVM
                     VPDLLFVPCVGFDADGFRLGYGGGYYDRTLAVWPGAKKPLTVGIAYEACRTDALQREA
                     HDIPLDLIVTEAGLYPRAA"
     gene            complement(2080..2592)
                     /locus_tag="VHV99_15825"
     CDS             complement(2080..2592)
                     /locus_tag="VHV99_15825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006024208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HEX3636926.1"
                     /translation="MSRHPTKIVSSEHLVSDTSAELSELEYALIMAGNAFNRWMVRCM
                     SAAGEKDMTAIEVSLLHHVSHRERRKKLADICFVLNIEDTHVATYALKKLVARGYVKS
                     EKTGKEVFFSATDAGRGLCLKYREVRESCLISTLKESGLTNEQIGEAAQLMRNASGLY
                     DTAARAAASL"
     gene            2800..3453
                     /gene="pxpB"
                     /locus_tag="VHV99_15830"
     CDS             2800..3453
                     /gene="pxpB"
                     /locus_tag="VHV99_15830"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006052562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HEX3636927.1"
                     /translation="MSQPRIFPLGDAALVCEAPPPATLDCQRRVWAAAEAARDWPHVL
                     EVVPGMNNLTLVFDPLEADREALASQLQTAWKAASEVPASGREVEIPVQYGGEFGPDL
                     QVVASHTGLSVREVVQRHADGEYVVFFLGFQPGFAYMGGLDQALHTPRRSSPRLEVPA
                     GSVGIGGEQTGIYPATSPGGWQLIGRTELPLFDPARRPPTLLQPGDRVRFTVAGIHA"
     gene            3450..4502
                     /locus_tag="VHV99_15835"
     CDS             3450..4502
                     /locus_tag="VHV99_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HEX3636928.1"
                     /translation="MIDVIRAGLLTTIQDLGRHGYRHLGVAMGGALDRLSLEVGNRLV
                     GNRPDAAGLEITFGPTVLRFLRATRVAITGTEFGATLDGKPVYSWWSLPVQAGQELVL
                     NAAKRGMRGYVCIAGGIDVLPMLGSRSTDLAGQFGGLGGRALRDGDRLPVGAPPQRGH
                     LGFTPEAPEFGVKAPAWCKFVLVHEPLRRGRHPSGVPWAVPIRVLQGPEYGSFTEAAH
                     ESFWSDEWLVTPNSNRMGYRLSGAELKRTQKSDLLSHAVLPGTIQVPPNGQPIVLMSD
                     AQTTGGYPKIGAVIQADMWKLAQVRLNAAIRFIPTTPYEARQALLEERTYLRQIDAAI
                     VMHEERCARQSAVAAL"
     gene            4626..5378
                     /gene="pxpA"
                     /locus_tag="VHV99_15840"
     CDS             4626..5378
                     /gene="pxpA"
                     /locus_tag="VHV99_15840"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HEX3636929.1"
                     /translation="MEIDLNADLGEGCGSDEALLDLVSSANIACGWHAGGANVMRDCV
                     RWAVQKGVSIGAHPSFNDPENFGRKEMDLPANDIYAGVLYQLGALSAIAQAEGGRIAH
                     VKPHGALYNQAARDAKIAEAIVSAVHDFDPSVAVFALANSGLVTAAREAGLTAVEEVF
                     ADRGYRADGSLVPRKEPGALLDDEDVVLERTLAMVRERRVQAVDGQWVPLNAQTICLH
                     GDGPHALAFARRIRGALQDAGIEVHAAGAANV"
     gene            5678..6427
                     /locus_tag="VHV99_15845"
     CDS             5678..6427
                     /locus_tag="VHV99_15845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEX3636930.1"
                     /translation="MQTTVSLWPLIGVAVIIVGFLLRFNPMLIVAVAAISTGLAAHFP
                     PEKILAEIGTGFIKTRNIPLIILLPLAVIGLLERHGLRERAQTWIGGIKAATAGRLLI
                     VYLLVRELTAAVGLTGLGGHPQMVRPLIAPMAEGATETRFGKISDAVRFKLRAFSAAT
                     DNVGLFFGEDIFVAFGAIVLMTTFLKEAGIIVEPIHVAVWGIPTAICAFIVHGFRLYL
                     LDRRLERELRGNAAANTAAVSPTQPAAGDKA"
     gene            6424..>7163
                     /locus_tag="VHV99_15850"
     CDS             6424..>7163
                     /locus_tag="VHV99_15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 family protein"
                     /protein_id="HEX3636931.1"
                     /translation="MTLTITYLFWLLGVVLLIIGGMIVTDKEHPRRFTAGGFWILYAL
                     IFLIGDKIPAAVVGVLVIVMALIAGFGGVTAAKPKLLSLEARKASAARLGNKLFVPAL
                     TIPVVTVIITLTASYLVFGGMPLIEKANVTLIGFGIGCVIALAIACVLTRDTVGQSMK
                     EARRLVDAFSWAAVLPQMLGMLGLVFSDAGVGKAVAHVTTAYISLDYRFVAVAVYCIG
                     MALFTMVMGNGFAAFPVMTGGVGVPILVG"
BASE COUNT         1213 a         2279 c         2342 g         1329 t
ORIGIN      
        1 tgcgcagcgg ccagttccat tcgcgattgc cctcgagccg cagattcagc gcatagaaac
       61 gctgcgccag atcgaagccc ggcgtgctgc cggcctgttg aacttcagcg tcgctcaccg
      121 tcgttttagg cggcacggtg atcttctggc cgagttcctc cgcagccagt tggccgtaga
      181 agttgaagcc ctgcgagatc gactcgaatt cctggttggc cgtggtcgta tcgccggctt
      241 gcttcaacgc acgtgcatgc cagtacaccc acgacggctg attgcgcagc gccgccggca
      301 tctgttcgat cgaccagcgc accatcgtcc agtcgcccgc gagcagcgcg ctgcgggtgc
      361 gccattcgta tgccgggttc gacagcggtg cattcgccga aagccggtac cagtcgaccg
      421 cgcccggcat ctgtttggcg gcggcctgat aggcaatcgt gccccaaccg atggcgcgct
      481 cgggcgagct cagcgacggc gccaccgagg cgaaagtggc cgcggccatg gccggatcgt
      541 tgcgcgccat gcgtgtgatc gcgagcaacg cgagttggtg cgactgtgtg tccggaccga
      601 cgccgcgagc cagcaacaac ggcggcgtgc tcgtggcctg gccgaacagc accggatcgg
      661 gcggctggtt gctcagtgca tcgaccagtt tgctgccggt gttggtgtag ttctgctcgt
      721 aggcgagacg gatctgctgc cagacgtcgt ccgcactgaa ttgctgattg accgccagcg
      781 aagtgatcag atcgacgcag ccgtcgccgt acacttttgg atcgaccagc agcgcacgtg
      841 ccgcgtcggc gacgttttca ccgcgcgcgg cgcgcgattc gagcgcgtag catttcacct
      901 gcgtatcgtc gttcagcacg aagcgcgcgt attgctgatc gaagttgcgc cagtcgtgac
      961 gcgcgccgag caccgtgagg taatcattgc gcaggcggtc ggcgatcgcc tggccgtcgt
     1021 atttctgcag gaacgccagc accggcgcgt ccggcgcgtc gatgcgcgca tggccgctgg
     1081 aatcgaacaa ctgtggcttg atctggaaat actcgagata cgacggcgcc gggtagttcg
     1141 ggatcatgct cgcgagttgc gcggcgcgtg ccgcgtcgtt attgcgggcg gcttcgcgaa
     1201 gctggacgaa agtttgatcg tcgttcgtga gttgcgaaag aggaatgggc ttgacggcgg
     1261 aggccgtgct gcacgcgacg agtgccgtgg cggcaagcgc cagaccggcc gcgcgatata
     1321 ctcgatagag gcgttttgac atcgttgttt ctggagcgcg aattgaaccc aagcatagca
     1381 tgcaaccccg tcgcggaatc gaaaaaggca ctgcgtcgaa tgctattgga aacaaggcta
     1441 caagcggctt ccgagccggc acacaacgcc gcgctcagcc gtcgcatgct cgacgcgttg
     1501 aagcactacg gcgtgcgccg cgtggggttc tattggccgc tggcgggcga gttcgacgca
     1561 cgcgccacga ttgcgatctg gcttgccgcc gactcgcaac gcgaggcgag tttgccgatc
     1621 gtcaaggacc gcggcgtgcc gctcgaattc cacgcatggg cgccggatac gccgatgaag
     1681 atcggccatc acaagatcgc cgagcccacc tcagggcgcg tgatggtacc cgacctattg
     1741 ttcgtgccgt gcgtcggttt cgacgccgac ggcttccggc ttggctacgg cggagggtat
     1801 tacgaccgca cactggctgt atggcccggc gcgaagaaac cgctcacggt cggcattgcc
     1861 tatgaagcgt gccgcacgga cgcgttgcag cgcgaggccc acgacatccc cctcgatctg
     1921 atcgtgacgg aagcaggcct gtatccgcga gcggcgtaat aagcggcccg ccgtctgacg
     1981 atctggcgtt cggtgaacgt cgcgcaaacc gtggcgttgt ggctgtggct gcggctatgg
     2041 ttgcggcccg gccgtgaccg agcgcacagc gacgacccgt tataaagacg ccgccgcccg
     2101 cgcggccgtg tcatacagtc cggacgcatt gcgcatcagt tgcgccgcct cgccgatctg
     2161 ctcgttggtc aggccacttt ccttcagcgt cgagatcagg caactctcgc gcacctcgcg
     2221 atacttcaga cagagcccgc ggcccgcatc ggtcgccgag aagaacactt ccttgccggt
     2281 cttttcgctt tttacatacc ctctagctac gagcttcttc aacgcgtagg tggcgacgtg
     2341 cgtgtcttcg atgttcagca cgaagcagat gtccgccagc ttcttgcgcc gctcgcgatg
     2401 gctgacgtgg tgcagcagcg agacttcgat ggcggtcatg tccttttccc cggcagccga
     2461 catgcagcgc accatccatc ggttgaacgc gttgcccgcc atgatgagcg cgtactccag
     2521 ctcggacagc tccgcgcttg tgtcagacac gagatgttcc gaggagacga tcttggtggg
     2581 atggcgcgac atggtgaaaa ttctcgagaa acaggttgga gaggtgtcgg gagtgtacga
     2641 cgagccactc cttcccggga gcctggcgaa aacgcttatt gagaaattgt tgatatttta
     2701 ttgataatgt acgcttcaag catcgtgcac gctatgccga taactatcca tagggttcga
     2761 tatgctcgac atttccgact cgacctgggc ccgtaaccga tgagccaacc aagaatcttt
     2821 ccgctgggcg acgccgcgct cgtctgcgag gcgccaccgc ctgccacgct ggactgccag
     2881 cggcgcgtgt gggccgccgc cgaggccgcg cgcgactggc cgcacgtgct ggaagtcgtg
     2941 ccgggcatga acaatctgac gctcgtattc gatccgctcg aagccgaccg cgaagcgctg
     3001 gcgagccagt tgcaaacagc ctggaaagcg gcgagcgagg tgcccgcgtc cggccgtgaa
     3061 gtcgaaatcc ccgtgcaata cggcggcgaa ttcggcccgg acctgcaagt ggtcgccagt
     3121 cacaccggcc tgagcgtgcg cgaagtcgtg caacgtcatg cggacggcga gtatgtagtg
     3181 tttttcctcg gcttccagcc gggctttgcg tatatgggcg ggcttgacca ggcgctgcac
     3241 acgccgcgcc gttcctcgcc gcggctcgaa gtgccggccg gttcggtcgg catcggcggt
     3301 gagcagaccg gcatttatcc ggcgacctcg cccggtggct ggcagttgat aggccgcacc
     3361 gagctgccgc tgttcgatcc ggcgcgccgg ccgcccacgc tgttgcaacc gggcgaccgg
     3421 gtgcgattta ccgttgcggg gatacacgca tgatcgatgt gattcgcgcg ggtctcctga
     3481 ccacgattca ggatctcggc cgccacggct accgccacct cggcgtcgca atgggtggcg
     3541 cgctcgaccg tctgtcgctc gaagtcggca accggctggt cggcaaccgg cccgatgcgg
     3601 ccggccttga aattaccttc ggcccaaccg tgctgcgctt tctgcgcgcc acccgcgtgg
     3661 ccatcaccgg caccgaattc ggcgcgacgc tcgacggcaa gccggtctat tcatggtgga
     3721 gcctgccggt gcaggccggc caggagctgg tgctgaacgc ggccaagcgc ggcatgcgcg
     3781 gctatgtgtg catcgccggc ggcatcgacg ttttgccgat gctcggctcg cgcagcaccg
     3841 atctcgccgg ccagttcggc ggactcggcg gtcgcgcgct gcgcgacggc gaccgcttgc
     3901 cggtcggcgc gccgccgcaa cgcggccacc tgggcttcac ccccgaagcg ccggagttcg
     3961 gcgtgaaggc gcccgcctgg tgcaagttcg tgctggttca cgagccgctg cggcgcgggc
     4021 ggcatccgtc cggcgtaccg tgggccgtgc cgatccgcgt gttgcaaggc cccgaatacg
     4081 gcagtttcac tgaagcagcg cacgaatcgt tctggtccga cgagtggctg gtcacgccca
     4141 atagcaaccg catgggctac cggctttcag gcgctgaact gaagcgcacc cagaaaagcg
     4201 atctgctgtc ccacgcggtg ttgcccggca cgattcaggt gccgccgaac ggccagccga
     4261 tcgtgctgat gagcgatgcg caaaccaccg gcggctatcc gaagatcggc gccgtgatcc
     4321 aggccgacat gtggaagctc gcgcaggttc gcctgaatgc cgccatccgc ttcatcccga
     4381 cgacgcccta tgaggcgcgt caggctttgc tggaagaacg cacgtatttg cggcagatcg
     4441 acgccgcgat tgtcatgcat gaagaacgct gcgcgcgcca atcggcggtc gcagcactct
     4501 aacaatgagc gcccccaaac cgggcgcttc gcaccaggcc ccccgagcgg gcagccccca
     4561 agggacctcc tgcggagcgg aaaacttggg gcggtccggc gttttcttga gagtagagga
     4621 acaccatgga aatcgatttg aacgccgacc tcggcgaagg ctgcggctcc gacgaggcgc
     4681 tgctcgacct cgtcagctcg gcgaacattg cgtgcggctg gcatgcgggc ggcgccaacg
     4741 ttatgcgcga ctgcgtacgc tgggcggtgc aaaaaggcgt gtcgatcggc gcgcatccga
     4801 gctttaacga tccggaaaat ttcggccgca aggagatgga cttgccggcc aacgacatct
     4861 acgcaggcgt gctctatcag ctcggcgcac tgtcggcgat cgctcaggcg gagggcgggc
     4921 gcattgcgca cgtcaagccg cacggcgcgc tctacaacca ggctgcgcgc gacgcgaaaa
     4981 tcgccgaggc gatcgtctcg gcagtccatg atttcgaccc ctcggtggcg gtgttcgcgc
     5041 tcgccaatag cggactcgtg acggctgctc gcgaagcggg cctcactgcc gtcgaagaag
     5101 tattcgccga ccgcggctat cgggccgatg gctcgctggt gccgcgcaag gagccgggcg
     5161 cgctgctcga tgatgaagac gtggtgctgg agcgcacgct cgcgatggtg cgcgagcgcc
     5221 gcgtccaggc agtcgacggg caatgggtac cgctcaacgc gcaaaccatc tgcctgcacg
     5281 gcgacggtcc gcacgcgctg gcgttcgcac gacgtattcg cggcgcgcta caggacgctg
     5341 gcattgaagt tcacgcggcc ggcgccgcga acgtctgatc cgcgcgtaga ccaacgcgcg
     5401 ccagtcagaa cgctcagtcg ttgccaaacc ccgcacgccc cgctccatgc ggggcgtttg
     5461 ccaggtgcag gacggattgc cgtaccggcg gtgacaagaa gtagttgcag ttgcagttgc
     5521 agttgcagtt gcagttgcag ttgcaggttg aagtgcaggt gtagtcgaag caacgcggcc
     5581 agcaagagcc gtgacagcaa gacggcgcgg ggtccctcgc gccggcccac gttagccgcc
     5641 ataagcggca aggttgcaac cctgtttgga gatccagatg cagacaacag tcagtctatg
     5701 gccgctcatt ggcgtggccg tcatcatcgt cggcttttta ttgcggttca atccgatgct
     5761 gatcgtggcg gttgccgcga tcagcacggg tctggccgcg cacttcccgc cggaaaagat
     5821 cctcgctgaa atcggcaccg gcttcatcaa gacccgtaac attccgttga tcattctgct
     5881 gccactggcc gtaatcggcc tgctggaaag gcacggtctg cgcgagcgcg cgcaaacctg
     5941 gatcggcggc atcaaggccg cgaccgcggg gcgtttgctg atcgtctatc tgctggtgcg
     6001 cgagctgacc gcggctgtcg gcctgaccgg tctcggcggc catccgcaga tggtgcgtcc
     6061 gctgatcgcg ccgatggccg aaggcgcgac tgaaacgcgt ttcggcaaaa tcagcgacgc
     6121 ggtgcgtttc aagctgcgcg ccttctccgc ggcaaccgac aacgtcggct tgttcttcgg
     6181 tgaagacatc ttcgtcgcgt tcggcgccat cgtgctgatg acgaccttcc tcaaggaggc
     6241 cggcatcatc gtcgagccga tccacgtggc cgtctggggt atccccaccg cgatctgcgc
     6301 gttcatcgtt cacggcttcc gtctgtatct gctcgaccgc aggctcgagc gcgaactgcg
     6361 aggcaatgcc gcagcgaaca ccgccgccgt ttcgccgacg caacccgcag caggagataa
     6421 agcatgacgc tcacgattac gtatctgttc tggctgctgg gcgtggtgct gctcatcatc
     6481 ggcggcatga tcgtcacgga caaggaacac ccgcgccgtt tcaccgccgg cggtttctgg
     6541 attctttacg cgctgatctt cctgatcggc gacaagatcc cggccgccgt agtcggcgtg
     6601 ctggtgatcg tgatggcgct gatcgcgggc tttggcggcg tgaccgcggc caagccgaag
     6661 ctgctgtcgc tcgaagcgcg caaggcgagc gccgcgcgcc tcggcaacaa gctgttcgtc
     6721 cccgcgctga cgattccggt cgtcaccgtg atcattacgc tgacggcgag ctacctggtt
     6781 ttcggcggca tgccattgat cgagaaggcc aacgtgacgc tgatcggctt cggcatcggt
     6841 tgcgtgatcg ccctcgcgat tgcctgcgtg ctgacgcgcg ataccgtcgg ccagtcgatg
     6901 aaagaggcac gccgcctcgt cgatgcgttc tcgtgggccg ccgtgctgcc gcagatgctc
     6961 ggcatgctcg gcctcgtgtt ctcggacgcg ggcgtcggca aggcggtcgc tcacgtcacc
     7021 accgcgtaca tcagcctcga ctaccgcttt gtcgcggtgg ccgtctattg catcggcatg
     7081 gcgctcttca cgatggtgat gggcaacggc ttcgctgcgt tcccggtgat gaccggcggc
     7141 gtcggcgtgc cgattctggt cgg
//
LOCUS       DATASH010000586         4770 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_96673,
            whole genome shotgun sequence.
ACCESSION   DATASH010000586 DATASH010000000
VERSION     DATASH010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4770
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96673"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1817)
                     /gene="ligD"
                     /locus_tag="VHV99_15855"
     CDS             complement(<1..1817)
                     /gene="ligD"
                     /locus_tag="VHV99_15855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018432046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX3636932.1"
                     /translation="MSDRLDLYNRKRRFDETPEPSGAAARKKAGRKTAARDANETLSY
                     VIQEHDARRLHYDFRLELNGTLLSWAVPKGPSLDPSVKRLAVHVEDHPVEYGSFEGEI
                     PPGNYGAGTVIVWDRGTWEPVGGATQAARSYEAGKLKFRLCGEKLHGGWTLVRSHMRG
                     SGDKEQWLLIKERDDEARDESEYDVLKKRPGSVLNGSASVGKSARSTQSTAGNAGVKR
                     AASANTASGANAATTSAKTVKTAPGAKDSPHSTTTGKGRAKAASTNRADPKKTDIVAT
                     RTTQSLLELAASPSIEGAVKAALPATFKPQLATLVDTAPPGDDWVYEIKFDGYRVLVR
                     IERKTKTRAVTVFTRAGNDWTAKFSKQVKAFERLDIESAWLDGEAVVLDQNGLPSFQA
                     LQNAFDSNRPQDIVIYLFDIPFLNGYDLRGVPLEQRRAILRALMDGHDKDRHDGKATD
                     DAVLRFSNDFAFSADDLLKSACDMALEGIIGKRRDSGYASGRSSAWIKLKCRRREEFV
                     IGGYSEPSGSRAAFGALLLGVHDGKGKLQYAGRVGTGFDAPLLRSVKKALDAHETKRM
                     PFASAPRERSRTPVHWVEPVLVCECNFAEWTSDGIVRQAS"
     gene            complement(1885..2895)
                     /locus_tag="VHV99_15860"
     CDS             complement(1885..2895)
                     /locus_tag="VHV99_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX3636933.1"
                     /translation="MAHMIWKGAISFGLVHVPVQLYPATQSEKVGFNLLDKRSIDPIG
                     YKQINKRTGKDVTRENIVRGFEYEKDKYVVLSDDEIRSANPESTQTVDILAFVDAPDI
                     SFLYLDTPYFLTPDRKGEKVYALLREAMKASGKIGVASVVLHNKQHLAALIPVGPVLA
                     LNTLRWAAEVRDFDEFKLPPEGMKAAGVTARELDMAKKLIDDMSDTWDPSQYHDTFRD
                     DIMALVDRKIRAGKTEEITEVEAPHESRQSADILDLSDLLKRSLGRGKGKPAAGARKR
                     AADDEDEAVDTDTDADEEPAAPARKKPRATRPASTSRSGGKSGGASAKVAPAARKRRA
                     AA"
     gene            complement(3101..3510)
                     /locus_tag="VHV99_15865"
                     /pseudo
     CDS             complement(3101..3510)
                     /locus_tag="VHV99_15865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432867.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
     gene            3625..4464
                     /gene="serB"
                     /locus_tag="VHV99_15870"
     CDS             3625..4464
                     /gene="serB"
                     /locus_tag="VHV99_15870"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007585839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEX3636934.1"
                     /translation="MNLVIQSTAPLSVDHHKTLVALARGSHAAVIDANAIRIADAKIA
                     QRADLEVYCGTHQLDYAFVEPRHPLRDFGLVAMDMDSTLITIECIDEIADFCGLKTEV
                     AAITEASMRGEIKDFNESLTRRVALLKGLDAGALERVYEERLTLSPGAEQMLAGAKAA
                     GLKTLLVSGGFTFFTEKLKARLGLDFTRANTLEIVDGKLTGNVIGEIVNADVKARTLR
                     ETCAQLGIEPTRAIAMGDGSNDLKMMAAAGLSVAFRAKPVVRQAASVAFNYVGLDGLL
                     RLF"
     gene            complement(4515..4646)
                     /locus_tag="VHV99_15875"
     CDS             complement(4515..4646)
                     /locus_tag="VHV99_15875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636935.1"
                     /translation="MDKLFTYLQSDENYTRVLKWHGAVIIITGISMLAMFMFADFKR"
BASE COUNT          802 a         1488 c         1484 g          996 t
ORIGIN      
        1 gatgcctgcc gcacgatgcc atcgctcgtc cattcagcaa aattgcattc gcagacgagt
       61 acgggctcga cccaatgcac cggcgtcctg ctgcgctcgc gcggcgcgct ggcgaacggc
      121 atgcgcttgg tctcgtgcgc atcgagcgcc ttcttgaccg agcgcagcaa cggcgcgtcg
      181 aagcccgtgc cgacacggcc ggcgtattgc aacttgcctt tgccgtcgtg cacgccgagc
      241 aacagcgcgc cgaatgccgc acggctgccc gagggctccg aatagccacc gatcacgaac
      301 tcctcgcgcc gccggcactt caatttgatc cacgcggacg agcgccccga tgcgtagccg
      361 ctgtcgcgcc gtttgccgat gatgccttcg agcgccatgt cgcatgcgct cttcagcaga
      421 tcgtcggcgc tgaacgcgaa gtcgttggag aagcgcaata cggcgtcgtc ggttgctttg
      481 ccgtcgtggc ggtccttgtc gtggccgtcc attagtgcgc gcaaaatcgc gcggcgttgt
      541 tctagcggca cgccgcgcaa gtcgtagccg ttcaggaacg gaatgtcaaa cagatagatg
      601 acgatatcct gcggacggtt cgagtcgaac gcgttttgca gcgcctgaaa actcggcagg
      661 ccgttttgat cgagcaccac ggcttcgccg tcgagccacg cactttcgat gtcgagtcgt
      721 tcgaacgcct tcacctgctt gctgaacttc gccgtccagt cgttgcccgc ccgcgtgaac
      781 accgtgaccg ccctggtctt cgtctttcgt tcgatacgga ccagcacgcg gtagccgtcg
      841 aacttgattt cgtataccca atcatcgccg ggtggcgcgg tgtcgacgag cgtcgccagt
      901 tggggtttga aggtggcggg caacgcggct ttaaccgcac cttcgatcga gggcgaggcg
      961 gcaagctcga gtagcgactg cgtggtgcgc gtggctacga tgtcggtttt tttggggtcg
     1021 gcgcggtttg ttgaagcggc ttttgcgcgg cccttaccgg tcgttgttga gtgtggggag
     1081 tcttttgcac ccggcgcggt ctttacggtc tttgcgctcg tggtcgcggc gtttgcgcca
     1141 ctggcggtgt ttgcacttgc agcacgcttg accccggcat tcccggctgt ggattgcgtg
     1201 gaccttgcgc ttttgcccac cgacgcactg ccgtttagca cactgcctgg acgtttcttc
     1261 aggacgtcat attcgctttc atcgcgggct tcgtcgtcgc gctccttgat cagcagccac
     1321 tgctccttat cgccgctgcc gcgcatatga ctgcgcacca gcgtccagcc gccgtgcagt
     1381 ttttcgccgc agagccggaa tttgagcttg cccgcctcat acgatctggc cgcctgcgtc
     1441 gcgccgccga ccggctccca cgtcccgcga tcccacacga tcaccgttcc cgcgccgtaa
     1501 ttgccaggcg ggatttcgcc ttcaaacgag ccgtattcga ccggatgatc ttcgacatgc
     1561 accgcgagcc gtttcaccga cgggtcgaga ctcggtccct tgggcaccgc ccatgacagc
     1621 aaggtgccgt tcagttcgag ccggaagtcg tagtgcaagc gccgcgcgtc gtgctcctga
     1681 atcacatacg agagcgtctc gttcgcatca cgcgccgcgg ttttgcggcc ggccttcttc
     1741 cgtgcggccg cgcccgacgg ctccggcgtt tcgtcgaaac ggcgcttgcg gttgtacaga
     1801 tcgagccggt cgctcatgga ggtggacctc gttgcgagtg gagccgtcgc ggtgtatcgg
     1861 gcaagtacgc gctcagttga gcgatcaggc ggccgcgcgg cgtttgcgtg ccgccggcgc
     1921 caccttcgcg ctggcgccgc cgctcttgcc gccgctgcgg ctcgtactcg ccgggcgcgt
     1981 cgcgcgcggc ttcttgcggg caggcgcggc tggctcctcg tcggcgtcgg tgtcggtgtc
     2041 gacggcttcg tcctcatcgt ccgcggcacg cttgcgcgcc cccgctgccg gcttgccttt
     2101 accgcggccg aggctgcgct tgagcaggtc ggacaaatcc agaatgtcgg cggattggcg
     2161 cgattcgtgc ggcgcctcga cctcggtgat ttcctcggtc ttacccgcgc gaatctttcg
     2221 gtcgaccagc gccatgatgt cgtcgcggaa cgtgtcgtga tactgcgagg gatcccacgt
     2281 gtcgctcatg tcgtcgatca gctttttcgc catgtcgagt tcgcgcgcgg tgacgccagc
     2341 ggccttcatt ccttcaggcg gcagcttgaa ttcgtcgaag tcgcgcactt cggcggccca
     2401 gcgcagcgtg ttcaatgcca acaccggtcc gactggaata agtgccgcga gatgctgctt
     2461 gttgtgcagc acgacgctcg ccacgccgat cttgcccgac gctttcatcg cttcgcgcag
     2521 caatgcatag accttttcgc ccttgcggtc gggtgtcaga aagtacgggg tgtcgagata
     2581 gagaaacgag atgtccgggg cgtcgacgaa ggcgaggata tcgaccgtct gcgtcgactc
     2641 gggattggcc gagcgaatct cgtcgtccga gaggacgaca tacttgtcct tttcgtactc
     2701 gaagccgcgc acgatgttct cgcgcgtcac atccttgccg gtgcgcttgt tgatctgctt
     2761 gtagccgatc ggatcgatcg agcgcttgtc cagcagattg aaaccgactt tctccgactg
     2821 cgtcgccgga tacaactgca ccggtacatg gacaaggccg aagctgattg cgcccttcca
     2881 gatcatgtgt gccatcgtgc gctccccaag gcctaggacc ttcggtgaag cagcaagcgt
     2941 gccacggctt tccggctatg actggcgcgg tgccgtactg accgtggcgg gtatcggctg
     3001 taagtcttgc gttatgtcgg caaaagatac ggagttttgt cgttgtggcg tggtggtggc
     3061 gtggttgtgc agcgcttatg ggtgtgcctg cggcgtgcgc tcatggcgaa cgtcggtatc
     3121 gggcccagct cgttggttcg agctcgaccg gcacttcggc gaagccgcga aacgcatgcg
     3181 gccgccacac gtcggctcgt cgccgagccg gtacgccgga aagcgctgca cgaagcgccc
     3241 gatggcaatg cgcgcctagc gtcgcgcgag ccacaagccc gcgcactggt gaatggcgaa
     3301 gccgaccgcc ggatgccggt tcggctcgcg gcggatgtca aaacggtccg gttgcgcgaa
     3361 ctgcccgggg tcgcgattgg ccgcgccgat gcacagtgtg acaggcgtac cgcctgcgcc
     3421 tgccaccgca acgccgccga tgtccgtgtt gagcgttgcc atccggttac gtggccggct
     3481 ccctgcagaa ttgcattgcg acacgcttgt cgcctgctcc ctggtcagtt gcgcccggcg
     3541 gaccgcgcgg cgccgacccg ttacaatgac gggattttcc gcgcgcgtcg cgcggccgcc
     3601 ctttcctgcg tcaatgcgtc gtccatgaac ctcgtcatcc aaagcaccgc gcccctttcc
     3661 gtcgaccacc acaaaacgct cgtcgcgctc gcgcgcggct cgcacgccgc cgtcatcgat
     3721 gcgaacgcga ttcgcattgc cgatgcgaag attgcgcagc gtgccgacct cgaagtctat
     3781 tgcggcacgc atcagctgga ctacgcgttt gtcgaacccc gccacccgtt gcgtgacttc
     3841 ggccttgttg cgatggacat ggactcgacg ctgatcacaa tcgaatgcat cgatgaaatc
     3901 gccgactttt gcgggttgaa aacagaagtg gccgcgatca cagaagcgtc gatgcgcggc
     3961 gaaatcaagg acttcaacga gagcctgacg cgccgcgtgg cactcctcaa gggactcgac
     4021 gccggcgcgc tcgaacgcgt ttatgaagaa cggctgacac tctcgccggg tgccgaacag
     4081 atgctggctg gcgcgaaagc ggcgggcttg aaaacgctgc tggtgtccgg cggcttcacg
     4141 ttcttcaccg aaaaactgaa ggcccggctc ggcctcgatt tcacgcgcgc gaacacactc
     4201 gaaatcgttg atggaaagtt gactggcaac gtgatcggcg agatcgtcaa cgccgatgtg
     4261 aaagcacgga cgttgcgcga gacatgcgct caactcggta tcgagccgac gcgcgcgatt
     4321 gcgatgggcg acggttcgaa cgatctgaaa atgatggccg ccgcgggact ttccgttgca
     4381 ttccgcgcga agccggtggt gcgccaagcg gccagcgtgg cgttcaacta cgttgggctg
     4441 gatgggttgc tgcgtctgtt ctgaagctta agttgcgctg gccgatgcag tggcggccag
     4501 cgcgggcagg aacactatct tttgaaatcc gcgaacatga acatggccag catgctgatc
     4561 ccagtgatga tgatcaccgc gccgtgccac ttcagaacac gagtgtagtt ttcgtccgat
     4621 tgcagatacg tgaataattt gtccattagc gcctcccaaa agtagatcag tataaaagat
     4681 gcgttatcta aaaaatctaa ttcaggtgca catgtaggat tacaaacagt acagcagcaa
     4741 gagatgttgc caaaatcgaa atcacgattg
//
LOCUS       DATASH010000587         3825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6030785,
            whole genome shotgun sequence.
ACCESSION   DATASH010000587 DATASH010000000
VERSION     DATASH010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6030785"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="VHV99_15880"
     CDS             complement(<1..959)
                     /locus_tag="VHV99_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX3636936.1"
                     /translation="MTSPLFGQMMDVPLLASSLLSHAARHFSDTEIVSRRIEGDIHRY
                     TWRDCEKRAKQLAQALIGLGVNPGERIGTLAWNGYRHLECYYGVTGMGAVCHTINPRL
                     FPDQVAFIVNHADDEYVAFDMTFATLVDIIAPQCPNVKGWIALGDETCISTHLADMAT
                     PALSYETLLAKYDGDYTWPVLDERSASFLCYTSGTTGNPKGALYTHRSTVLHAFAASL
                     PDAMGCSARDAILPVVPMFHVNAWGIPHAAALVGAKLVLPGNCLDGKALYELMEAEGV
                     TFSAGVPTVWLGLLNYMKQAGVRFNTLKRTVIGGSACPPAMLRT"
     gene            1137..2735
                     /locus_tag="VHV99_15885"
     CDS             1137..2735
                     /locus_tag="VHV99_15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015601524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HEX3636937.1"
                     /translation="MSFSPSSTVAQADPAGLASVDSPVDSLLASIETNRPDAFVSLGS
                     AFLTRLPAAPLPEPYLVGISADTAAQLGFDASNAQDPAFAEPFAELFAGNPTREWPAE
                     KLPYASVYSGHQFGVWAGQLGDGRAIGLGEIEHDGKRLELQLKGAGRTPYSRMGDGRA
                     VLRSSIREYLCSEAMHHLGIPTTRALCVIGSDQPVRRETMETAAVVTRVAPSFVRFGH
                     FEHFYTNDRLDELKKLADHVIDRFYPQCRAADDPALALLQEVLNRTADLMADWQAVGF
                     CHGVMNTDNMSILGLTIDYGPFGFIDGFNANHICNHSDSQGRYAYKMQPQIGYWNLFC
                     LAQALVPLFGPQYDADTRNDKVIEDAQRLLEGFKDRFAPALERRMRAKLGLAEIREGD
                     DKLANRLFEVMNANRADFTLTFRNLAKMRKADASGDAPVRDLFLDRAAFDVWAADYRL
                     RLSEETQSDEARASAMNAVNPKYVLRNHLAENAIRLAKEKDFSEVERLAKVLRHPFDE
                     QPEFEAYAGLPPDWASDLEVSCSS"
     gene            2823..3245
                     /gene="msrB"
                     /locus_tag="VHV99_15890"
     CDS             2823..3245
                     /gene="msrB"
                     /locus_tag="VHV99_15890"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX3636938.1"
                     /translation="MNRDDLTKDPVAPLRKDDAEYRKELTDIQYQVTRHAATERPFTG
                     EYWDHTERGVYDCVCCGTPLFESDTKFDAGCGWPSYFKPINGEVIKEKTDRAHGMLRI
                     EVQCKNCGAHLGHVFEDGPAPTGLRYCINSAALQFESK"
     gene            3455..>3825
                     /locus_tag="VHV99_15895"
     CDS             3455..>3825
                     /locus_tag="VHV99_15895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HEX3636939.1"
                     /translation="MKFLFDLFPILLFFVAFKIWGIYTATAVAIVATLVQIAWVAFRH
                     RKVDPMLWVSLGVVVVFGGATLVLHNDTFIKWKPTALYWLFAGALIVSQAGFGKNLIE
                     AMMGKQIVLPHRIWGQLNLAWA"
BASE COUNT          769 a         1125 c         1159 g          772 t
ORIGIN      
        1 gtgcgcagca tcgcgggcgg acacgccgag ccgccgatca ccgtgcgttt gagcgtattg
       61 aagcgcacgc ccgcctgttt catgtagttc agcaagccga gccagaccgt tggcacgccg
      121 gccgaaaacg tcacgccttc ggcttccatg agttcgtaga gcgctttgcc atcgaggcaa
      181 ttgccgggca gcacaagctt cgcaccgacc agcgccgcgg catgcggaat accccacgcg
      241 ttgacgtgga acataggcac gacgggcagg attgcatcgc gggcggaaca gcccatggcg
      301 tcgggcagcg acgcggcaaa cgcatgcaac acggtcgatc gatgcgtgta aagcgcgccc
      361 ttcggattgc cagtcgtacc cgatgtatag caaagaaacg atgccgagcg ttcgtcgagg
      421 accggccacg tgtagtcgcc atcgtatttc gccagcagcg tttcgtagct gagcgcgggc
      481 gtggccatat cggcgagatg ggtgctgatg caggtctcgt cgccgagcgc aatccagccc
      541 ttcacgttcg gacactgcgg cgcaatgatg tcgacgagcg tggcgaacgt catgtcgaac
      601 gcgacgtatt cgtcatcggc gtgattgacg atgaacgcaa cttgatcagg aaagagccgc
      661 gggttgatcg tgtggcagac ggcgcccatg ccggtcacgc cgtaataaca ctccagatgg
      721 cgatacccgt tccacgccaa tgtgccaatg cgttcgccgg ggttcacacc cagcccgatc
      781 aaggcctgcg ccagttgttt tgcacgcttt tcgcaatcgc gccaggtgta gcgatgaatg
      841 tcgccttcaa tccggcgcga gacaatctcg gtgtcgctga aatggcgtgc ggcgtgggat
      901 aacaacgatg aagcgagcag cggtacatcc atcatctggc cgaaaagcgg cgacgtcatg
      961 ggcgagttcc tcacaagtcc ggatgcacgg gttttgatgc ctgatctact acctgagaca
     1021 gtacctcaat tcgacactga gttgatcccc gatccaatgc cggggcgggt tgacgcgcgc
     1081 tcgtgcagcg gcgcgggggc ggatttacaa tatcggttaa tcaagaggtg ctcaatatgt
     1141 cgttttcccc aagcagcacc gtcgcgcaag ccgatccggc aggccttgcg tccgtcgatt
     1201 cacccgtgga ttcgctgctc gcatcgatag aaaccaacag gcccgatgcg ttcgtttcac
     1261 tcggctccgc ctttctcacc cgcctgcccg ctgcaccatt gcccgagcct tatctggtcg
     1321 gcatttccgc cgataccgcc gcgcaactgg gttttgacgc cagcaacgcg caagatcccg
     1381 cgttcgccga gccgtttgca gagctttttg cgggcaatcc cacacgggaa tggccggcgg
     1441 aaaaactgcc gtatgcgtcg gtgtattcgg ggcatcagtt tggcgtgtgg gccgggcagc
     1501 tcggcgacgg ccgcgcaatc ggcctgggtg aaatcgagca tgacggcaag cgcctggagc
     1561 tacagctaaa aggcgccgga cgcacgccgt attcgcgcat gggcgacggg cgggctgtgt
     1621 tgcggtcatc gatacgcgaa tatttgtgct ccgaagccat gcaccacctc ggcattccaa
     1681 ctacgcgggc gttgtgcgtc attggatcgg accagcccgt gcgccgcgaa acgatggaaa
     1741 cagccgccgt tgtgacgcgg gtcgcgccga gtttcgtgcg ctttggccac ttcgagcact
     1801 tctacacgaa tgatcggctc gacgaattga agaagctcgc cgatcacgtg atcgaccggt
     1861 tctatcccca gtgccgcgca gccgacgatc ccgcgttggc gctcttgcag gaagtcctga
     1921 atcgcacagc cgatttgatg gcggactggc aagctgtcgg gttttgccat ggcgtgatga
     1981 acacggacaa catgtcgatc ctcggattga ctatcgacta cggcccgttt ggctttatcg
     2041 atggattcaa cgcgaatcac atctgcaatc actctgattc gcagggtcgg tacgcttata
     2101 aaatgcagcc gcagattggc tactggaatc tgttttgcct cgcgcaggcg ctggtgccgc
     2161 tattcggtcc gcaatacgac gccgacactc gcaacgacaa ggtgatcgaa gacgcgcagc
     2221 ggctcctgga aggattcaag gaccgttttg cgcccgcgct tgagcgccgc atgcgtgcga
     2281 agctcggtct ggcggagatt cgcgaaggtg acgacaaact cgcgaaccgt ctgttcgaag
     2341 tgatgaacgc caatcgtgcc gatttcacgc tgacgttccg aaacctggcc aaaatgcgca
     2401 aggcggacgc ttctggcgac gcgccggtgc gtgatctgtt cctcgatcgc gccgctttcg
     2461 atgtctgggc ggccgactac cggttgcgct tgtcggagga aacacaaagc gatgaagcgc
     2521 gggcaagcgc gatgaacgcc gtgaatccaa aatatgtctt acgcaatcat ttggccgaga
     2581 acgccatccg cctggcaaaa gagaaggatt tttccgaagt tgaacggctg gcaaaggtgt
     2641 tgcgccaccc gttcgatgaa caacctgaat ttgaagcgta tgccggcctg ccgcccgact
     2701 gggcgagcga cctggaagtt agttgctctt cctgaaatag cccccaaata catgagccca
     2761 aacgagcccg agcgagccgc tttccttgtt gggctccgct aaagatctgg agatagagaa
     2821 ccatgaaccg agacgatctg acgaaagacc ccgttgctcc gctgcggaag gacgatgccg
     2881 agtatcgaaa ggaattaacc gatatccagt accaggtgac gcgtcacgcc gccacggaac
     2941 ggccgttcac cggcgagtac tgggatcaca cggaacgcgg tgtgtatgac tgcgtgtgct
     3001 gcggcacgcc gctgttcgag tcggatacga aattcgatgc cggttgcggc tggccgagct
     3061 acttcaagcc gatcaacggc gaagtcatca aggaaaagac ggatcgtgcg cacggcatgc
     3121 tgcgcatcga ggtgcaatgc aagaactgcg gcgcgcatct gggccacgtt ttcgaagacg
     3181 gtcccgcgcc gaccggtctg cggtactgca tcaactcggc tgcgctacaa ttcgaatcca
     3241 agtaagcgcg tcgggcgtcg ccggccagtg agccggcaag tcggcgttga cgcggcgagc
     3301 gatctcgccg cgcgctttca agtgctgctt tttacgtgct aaacgcggct gactgcggct
     3361 attcactcgc caggcgtcgt gctgtgccgg tagcgaaccg gcgacacacg gcgcgtgcgc
     3421 gcttccatat tgatgccctc ctcgtcccta cgacatgaaa tttctgttcg atctatttcc
     3481 gatcctcctc ttcttcgtcg cattcaagat ctggggcatc tacaccgcca ccgctgtcgc
     3541 cattgtcgcc acgcttgtgc agatcgcgtg ggtcgcgttc cggcatcgca aggtcgatcc
     3601 catgttgtgg gtgagcctgg gtgtggtggt cgtattcggc ggcgcgacgc tcgtgctgca
     3661 taacgacacg ttcatcaaat ggaaacccac cgcgctttac tggctgtttg ccggtgcgct
     3721 gattgtgtcg caggccggtt tcggcaagaa cctcattgaa gcgatgatgg gcaagcagat
     3781 tgtgttgccg catcgcatat ggggccaatt gaaccttgcc tgggc
//
LOCUS       DATASH010000588        10117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1878871,
            whole genome shotgun sequence.
ACCESSION   DATASH010000588 DATASH010000000
VERSION     DATASH010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 10117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10117
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1878871"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(284..517)
                     /locus_tag="VHV99_15900"
     CDS             complement(284..517)
                     /locus_tag="VHV99_15900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636940.1"
                     /translation="MRRTSTRIRLVATPEEKKPPSQLQLQLQLQLQLQLQLQLQVALA
                     LALALAVAFAVAVAVAVAVAVHQGYAMPSHGSG"
     gene            complement(1099..1899)
                     /gene="iolB"
                     /locus_tag="VHV99_15905"
     CDS             complement(1099..1899)
                     /gene="iolB"
                     /locus_tag="VHV99_15905"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HEX3636941.1"
                     /translation="MSLLVKAQREGQTIVRVTPESACWRYVGFAAYRLGVDEVVHVFE
                     PSREVCIVVLTGAVDIETADAKWRSLGSRDSVFEDSAPYALYLPPGVRATVRACRDAE
                     VGVASAPGKGAYPARLIEPSTMKRSTRGKGLNTRYVCDILPQTELAESLLVVEVRTPG
                     GHASSYPPHKHDTDNVPHESSLEETYYHRLDPPQGFAFQRVYTDMRDIDESMAVENHD
                     VVMVPRGYHPVVVPYGYESYYLNVMAGGQRVWHFRNDPAHEWIVNKDS"
     gene            complement(1896..2822)
                     /gene="iolE"
                     /locus_tag="VHV99_15910"
     CDS             complement(1896..2822)
                     /gene="iolE"
                     /locus_tag="VHV99_15910"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HEX3636942.1"
                     /translation="MTAFDVRIGINPLSWMNDDLPSLGGETPLEVALTEGRQIGYQGF
                     ELGNKFPREPQALKTLLAQYDLALVSGWYSGRLARRSVAEEVAAVGPHLELLAQNGAT
                     VMVYGEVADSIQGAPQPLYQRPRFFSDAQWAAYAARVDEFARYTLGRGVRLAYHHHMG
                     AYVETPADVDQLMSRTSDAVGLLFDAGHITFAGGDPLAVLDQHIGRVCHVHCKDVRPA
                     VMKLARNRNWSFLDAVINGAFTVPGDGAVNFAALIDRLKRHGYRGWLVVEAEQDPVVA
                     PSYDYAEKGFRTFRALVDAPRGAGDETEREAA"
     gene            complement(2833..4806)
                     /gene="iolD"
                     /locus_tag="VHV99_15915"
     CDS             complement(2833..4806)
                     /gene="iolD"
                     /locus_tag="VHV99_15915"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HEX3636943.1"
                     /translation="MNERILHHDAASANDAIQARAPSGGTLRLTTAQALVRYLAAQRV
                     TSEDGSGIEPLFGGVFAIFGHGNVAGMGEALYQHREALPTLRAHNEQAMAHSAIAYAK
                     AHFRRRMMAVTTSIGPGATNLITAAALAHVNRLPVLLLPGDVFISRAPDPVLQQVEDF
                     HDGGVSANDAFKPVSRYFDRIVHPAQLLNALPRALRVLTDAALCGPVTLALPQDVQAQ
                     AWDFPADFFAPRVVTFHSPGPRAEDIDAAVARLRRARRPLIVAGGGVLYGRATEALHR
                     FAAAHGVPVAETQAGKGSLAWNDPLNAGALGVTGSPAANALAHDADCVLAIGTRLQDF
                     TTGSNTLFTQADVIGINANPFDGLKHRALVLEADARLALDALAEPLQGWHADRTWTAR
                     AHKLATSWRDTVGTLTHAPQRDTVLPYEGDVIGAVQRSSARSTMDDIVVCAAGTLPAE
                     LHKLWRAGKPGAYHVEYGYSCMGYEIAGGLGVKLARPAREVIVMLGDGSYLMMNSEIA
                     TSVMLGAKLIILVLDNRGYGCINRLQQACGGAPFNNLLDDCMQGPLGAPRVDFAAHAR
                     ALGAQAEHVANIAELENALQRARAADRTYVISIDTDPARTTDDGGWWWEVAVPEVSAR
                     DTVRAARAQYDAQLAARAAPADPGERPGHIDTE"
     gene            complement(4803..6881)
                     /gene="iolC"
                     /locus_tag="VHV99_15920"
                     /pseudo
     CDS             complement(4803..6881)
                     /gene="iolC"
                     /locus_tag="VHV99_15920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433791.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
     gene            complement(6948..7787)
                     /locus_tag="VHV99_15925"
     CDS             complement(6948..7787)
                     /locus_tag="VHV99_15925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3636944.1"
                     /translation="MSDTNTVSTVNTAGTSEPQDDVILALEDVSKYFGQVIALSGVTL
                     RLKRGEVHCLLGDNGAGKSTLIKTLAGVHQPSSGQYLVDGKPVLFESPKDALDLGIAT
                     VYQDLALVPLLSVARNFFMGREPQKKLFGFLNVMDLETSATTARDKLAEMGINVRDPH
                     QPIGTMSGGEKQCLAIARAIHFGARVLILDEPTAALGVKQSFNVLKLIHTARAKGISV
                     IFITHNVHHAYPIGDSFTLLNRGKSLGTFTKDTISKDEVLDMMAGGAEMQKMIGELEG
                     TTI"
     gene            complement(7801..8994)
                     /locus_tag="VHV99_15930"
     CDS             complement(7801..8994)
                     /locus_tag="VHV99_15930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017777731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3636945.1"
                     /translation="MGVANPFHPHPRKQPPSGAGTAATPNSAPASATGAAGADERVRQ
                     ESWFRHLLNRPEFAALAGTVMVFLVFGIAAGNSGMFNLDGVMNWSQVAAYLGLIAVGA
                     CLLMIAGEFDLSIGSMIGFSGMMVALPTMYFHWPIALSILFAFAGSMLLGALNGYLVM
                     RTRLPSFIVTLAFLFILRGLTLALSVMFADRTIISGVGDVAAHDWLAHTLFQGVAFKG
                     LFIWLGQLGVVKLLDNGNPLVPGIPKVLLWWFALAAVCAFTLAKTRFGNWIFAVGGDA
                     NAAKNVGVPVRRVKISLFVLTAFCACLFAVLQVCDIGSAAADRGLQAEFEAIIAAVIG
                     GTLLTGGYGSVIGACFGALIFGVVQIGITYTNVDSDWFRVFLGVMLLIAVLFNHYVRR
                     RVAQS"
     gene            complement(9184..>10117)
                     /locus_tag="VHV99_15935"
     CDS             complement(9184..>10117)
                     /locus_tag="VHV99_15935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006026469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX3636946.1"
                     /translation="ISHAPDSDSWWNTIKNAIKQADEDFNVETDYRNPPNGDIADMAR
                     LIEQAAARNYDGVIVSIADFDVLKSAIAKVTEKHIPLVTINSGTEAQSAQLGAIMHVG
                     QPEYTAGKAAGEKAKAAGIKSFLCVNHYATNPASFERCRGFAEAIGVDFKSSTLDAGQ
                     DPTGVQSKVSAYLRNHPNTQAVLTLGPLSAAPTIKALQQMGLAGKVWFATFDFDNDIA
                     KGIQDGTIQFAIDQQPYLQGYIPVAVLAIVKKEHTTDPAKIRQILEANPKFKQRLATY
                     GLAPAYGPRNIGSGPGFITKANIDKVVKYAGQYR"
BASE COUNT         1960 a         3212 c         3168 g         1777 t
ORIGIN      
        1 aaagcggctc accccgccag cgcataagcg ggtcccccgc gcagtcgcgg tagtggtgca
       61 tctggaatct gtgccctcgc acattcagcc ctggtgacaa ggcagtcata cttcccgcct
      121 cgcactgcgt gctcgccgga agggttcatc aggaaggccg gtggcttcgc tcggacttag
      181 tgaaccatcc gtttccattc gataaagcca tcgtacttcc gacggcgccg cgcgcgccga
      241 aacgtgcttc ataaaaccac ccaccgcgtt tcacgcccac gttttacccg ctgccgtggc
      301 tcggcatcgc ataaccctga tgcacagcaa cagcaacagc aacagcaact gcaacagcaa
      361 atgcgactgc gagtgcaagt gcaagtgcaa gtgcaacctg caactgcaac tgcaactgca
      421 actgcaactg caactgcaac tgcaactgca actgcgacgg tggcttcttt tcttcgggcg
      481 tggctacgag gcgaatgcga gtggaggtgc gacgcactga catttcattt gaagtgcggt
      541 ggaccccgag tgtggtgcgg ctcaccgagg cgaagccgat ggctcccacg gggcctaagc
      601 gaagccgctg gtttgcgtga tgaaccgttc cggcgagcac gcagtgcgag gcgggaagta
      661 tgagtgcctt gtcacttgcg ctgaatgtgc gagaacacgg attccagatg caccactacc
      721 gcgactgcgc gggggacccg cttatgagct ggcggggtga gccgctttct ttgcttatct
      781 ttctttgcgg cggcaaagaa agtaagtgcc gccccgcaca ggggcaacac taatagacca
      841 atatcaattc aaggagaggc caaagaccca gatcaaggaa aggccaaaac cgtaggcata
      901 cgacctacaa gcgccgcaca cgcaccgagc gcaaggagac gccaacgccg cgaacacacc
      961 acccaaaagc accgcgagat caagaaaagg cctacgccat aaaagcccag acaaaaaccg
     1021 ccgcgcaggc aaccacatca aggaaagccc accgccacaa aaccgcagac aaacacccgc
     1081 tccgcaggaa aaaaacagtc aactatcctt attaacaatc cactcatgcg caggatcatt
     1141 acgaaaatgc cacacgcgct gtcctcccgc catcacattc aaataataag actcataccc
     1201 atagggaaca acaaccggat gatacccacg cggcaccatc acgacatcgt gattctcgac
     1261 agccatcgac tcatcgatat cacgcatgtc ggtatacaca cgctgaaaag caaacccttg
     1321 cggcggatcg agccgatgat aataagtctc ttcgagagaa ctctcatgag gcacattatc
     1381 cgtatcgtgc ttatgcggcg gatagctgga agcatgccca ccgggtgtcc gaacctcaac
     1441 aaccagcaaa gactccgcaa gctcagtctg cggaagaata tcgcatacgt atcgtgtatt
     1501 cagccccttg ccacgcgtgg agcgcttcat cgtggaaggt tcgatcaacc tcgccggata
     1561 cgcccccttg ccgggcgcac tcgccacgcc aacctccgca tcgcgacagg cgcgaaccgt
     1621 cgcccgcacg ccaggcggca aatacagcgc atacggcgcg gaatcctcga acacgctatc
     1681 gcgcgaaccc agcgaacgcc atttggcatc agccgtttca atatccaccg cgcccgtcag
     1741 caccacgata cacacttcac gggacggctc gaaaacatgc acgacttcat ccacgcccag
     1801 ccggtaagca gcaaacccca cataccgcca gcacgccgac tcaggcgtca cccgcacgat
     1861 tgtctggccc tcgcgctgcg ccttcaccaa taaactcatg ccgcctcccg ctcagtctcg
     1921 tcaccggcgc cacgcggcgc atcgaccagc gcccgaaagg tccgaaagcc tttctccgca
     1981 tagtcgtacg acggcgccac caccgggtcc tgttccgctt caaccaccag ccagccgcga
     2041 taaccatgcc gcttcaatcg atcgatgagc gcggcaaagt tcaccgcgcc atcgcccggt
     2101 accgtgaatg cgccgttgat cacggcatcg agaaaactcc agttccgatt gcgtgccagc
     2161 ttcatcaccg ccggccgaac gtccttgcag tgcacatgac atacgcggcc gatgtgctgg
     2221 tcgagtaccg cgagcggatc gccgccggca aacgtgatgt gccccgcatc gaatagcaaa
     2281 cccacggcgt cgctcgtgcg cgacatcaac tggtcgacgt cggccggcgt ttcgacatac
     2341 gcgcccatgt gatggtggta cgcgagccgt acgccgcggc ccagcgtgta gcgcgcgaat
     2401 tcatcgacgc gcgccgcata ggcagcccac tgcgcgtcgc tgaaaaaacg cggacgttga
     2461 tacagcggct gcggcgcgcc ctggatcgaa tcggcgactt cgccgtagac catcaccgtc
     2521 gcgccgttct gcgcgagcag ttcgaggtgc gggccgaccg cggcgacttc ctctgccacg
     2581 ctgcgtcgcg cgagtcgtcc ggagtaccag ccggatacca aggccagatc gtattgggca
     2641 agcaacgtct tcaacgcctg tggttcgcgc ggaaatttgt tgccgagctc gaacccctga
     2701 tagccgatct ggcgcccttc ggtgagcgcg acttcgagcg gcgtttcgcc gccgagcgaa
     2761 gggaggtcgt cgttcatcca cgataaggga ttgatgccga tacgaacgtc gaaagcagtc
     2821 atgcgagggt cctcattcgg tgtcgatgtg gccggggcgt tcgccaggat cggcgggcgc
     2881 ggcgcgtgcg gcaagctgcg cgtcgtattg cgcacgtgcg gcacgcacgg tgtcgcgcgc
     2941 cgatacctcg ggcacggcga cttcccacca ccagccgccg tcgtcagtgg tgcgggcggg
     3001 gtcggtgtcg atgctgatga cgtaggtgcg atcggcggcg cgcgcgcgtt gcagtgcgtt
     3061 ttcaagttca gcaatgttcg cgacgtgttc agcctgcgca ccgagcgcgc gtgcatgcgc
     3121 ggcgaagtcg accctcggtg cgccgagcgg gccctgcatg cagtcgtcga gcaggttgtt
     3181 gaacggcgcg ccaccacagg cctgctgcaa gcggttgata cagccgtagc cgcgattgtc
     3241 gagcaccagg atgatgagct tcgcgccgag catcaccgac gtcgcgatct cgctgttcat
     3301 catcagatag ctgccgtcgc cgagcatcac gatcacttcg cgcgcgggcc gcgcgagctt
     3361 cacgccgagg ccgccggcga tttcgtagcc catgcacgag tagccgtatt cgacgtggta
     3421 cgcccccggt ttgcccgcgc gccagagctt gtgcaattcg gccggcaagg ttcccgctgc
     3481 gcaaacgacg atgtcgtcca tcgtggaacg cgcgctcgaa cgctgcaccg cgccgatcac
     3541 gtcgccttca tacggcagca cggtgtcgcg ttgcggcgca tgcgtgagcg tgcccacggt
     3601 atcgcgccag ctcgtggcga gtttgtgcgc gcgcgcggtc catgtgcggt ccgcgtgcca
     3661 gccttgcagg ggttcggcga gcgcatccag tgcaagacgg gcatcggctt cgagcaccag
     3721 cgcgcgatgc ttgaggccgt cgaatggatt cgcattgatg ccgatcacgt cggcctgcgt
     3781 gaacaaggtg ttcgaaccgg tggtgaagtc ctgcaagcgt gtgccgattg cgagcacgca
     3841 gtccgcatcg tgtgcgagcg cattggcggc aggcgagccg gtcacgccca atgcgccggc
     3901 gttcaatgga tcgttccacg cgagcgaacc cttgccggcc tgcgtttccg ccaccggaac
     3961 gccgtgcgcg gcggcgaagc ggtgcagcgc ctcggtcgcg cggccataga gcacgccacc
     4021 gcccgcgacg atcagcggcc gcctggcgcg gcgcagacgc gcgaccgccg cgtcgatgtc
     4081 ttcggcacga ggcccggggg agtgaaacgt aacgacgcgc ggtgcaaaga aatcagcagg
     4141 gaaatcccat gcctgcgctt gcacgtcctg gggcaacgcg agcgtgacgg gaccgcacaa
     4201 cgcggcgtcg gtgagcacac gcaaggcgcg tggcaatgca ttgagcaact gcgccggatg
     4261 cacgatgcga tcgaagtagc gtgacacggg cttgaacgca tcgttggcgg agacgccgcc
     4321 gtcatgaaaa tcttccacct gctgcaacac cggatcgggc gcgcgcgaga tgaacacgtc
     4381 gccgggcaac agcagcacgg gcaggcgatt cacatgcgcg agcgcggcag ccgtgatcag
     4441 attggtcgcg ccgggtccga tcgaagtcgt gacggccatc atacggcggc gaaaatgcgc
     4501 tttggcgtag gcgatcgcac tgtgcgccat cgcctgttcg ttatgggcgc gcagggtggg
     4561 caatgcttcg cggtgctgat acaacgcttc gcccatgccg gccacattgc cgtgtccgaa
     4621 gatcgcgaac acgccgccga agagcggctc aatgccgctg ccgtcttcgc tcgtgacgcg
     4681 ttgcgcggcg aggtagcgca ccagcgcctg agcggttgtc agacggaggg tgccgcccga
     4741 gggcgcgcgc gcctggatgg cgtcgttggc ggacgccgca tcgtgatgca atatgcgctc
     4801 gttcatgccg cctgctcctg atgtgcatgg cgcgctgtgg taagcggcgc gtttgccgct
     4861 ccgcgcgccg cacgccacga cgcgatcagc gtttcgaagg tgcggcgcac gcgcgcgatc
     4921 agttcgtcgt cgccgatttc tcccgcgagc catgcatggc tcggttcgtg aaaaatcgtg
     4981 cggcccacgg tgaagccccg gcatgtcgcc gattgtgccg ccgcgcgaaa gccctcgttc
     5041 agttgctcaa cgcccgccga caaaccgagc aataccacgc cccggcagta cggatcgcgc
     5101 tccgcgatca acgcgtcgac ggcttgccat tgcgcggcgt ccatgggttc gagcttccac
     5161 cattccggat agatgccgat gttgtacaac cgcttcaacg cgcgatagac gatgtccggt
     5221 gcttgcggca gatgcgcgtg cttcggcgga atcacttcga gcagcaattc gtggccgctc
     5281 gcttgcgtcg cgtcgtaaag cgcgcgcagt tgtgcctcct gttcgaggcg ttgttcgacc
     5341 ggttcatcgg gatggaattg caccaggcac ttggcaatgt gttcctgcgg ccacgcgatc
     5401 agcgcggtgc cgaccaagcg gccgtggtcg aatacgagcg gcacggaccc gggcagttca
     5461 accggccggc cgatccacca gccgcggccg gtggcggcgt tcaatgcgtc ctgaccgtag
     5521 cggtcgtcgc tcaatacgcc gatgcggccc tgcaacccca aggcgctttc cgtctgcgcg
     5581 acggcctcca cgaacaggcc cttcagttgc gcgatgcgcg cttcgtcggc accggtctgc
     5641 tgggcgagtt cgaagaactg gttgcggtgg tcgaacgcga agcccagtac ttcgtcccat
     5701 tgtttgcgcg caggggagac acgatgcagg cgtgcgagcg ccgcatcgcg gtcggggcgg
     5761 cgcatgcgtt gcggatcggc tttggcttcg cgcaggaagt agtcgagtta gacgggcgtc
     5821 ggcatcgcgg gcgcgcagcc gtgacgcgag acgaccagcg caccgctggc attcgcggcg
     5881 cgcgcgcagg cttcgagtgg ctggtcgcgc aaccatccgg aaagaaagcc ggacgcgaac
     5941 gcgtcgcccg cgccgagcac gttgagcact tcgacttcca cgccgctatg aatcggtaaa
     6001 tcgtcaagcg ttgccggaac cttgccgtcg atgatctggc agcccatcgg cccgcgcttc
     6061 aggacgagcg tcgcaggtgt cacggcacgc accatcgcga gtgcgtcgac gagttcggtc
     6121 ttgccgccgg cgatgcggaa ctcctcctcg gtgccgatca ccagatcgaa caagggcaga
     6181 atgcgctgca agtgcgcgct cacgccttcg ctcgcgacga agcgggtttc gccgtccgct
     6241 ttgcccgtga gaccccacaa cacagggcga taatcgatat cgagcacggt gcgcacgtta
     6301 ttgcgccgtg cgtgatcgag cgcgcgaccg cttgtgcggt tcacctgccc ggtcgaaaaa
     6361 tgcgtgccgg taatcagcag cgctttcgac gacgcgatgt acgcctcgtc gaaatctgct
     6421 tcatccaccg ccatatcggc gcagttctca cgatagaaga tcagcggaaa cgtatcgcga
     6481 tctttcaggc cgagcagaac gagcgcggtc aggcgcgcct gatcgatacg tacatgactg
     6541 acgtcgcagc cttccttcgt caaggtctcg gtgagaaagc ggcccatgtg atcgttgccg
     6601 acacgcgcca gcatcgccga tcgaagcccg agccgcgcgc aaccgaacgc gatattcgcc
     6661 gaggacccgc cgagatactt cgcgaagctc gacacgtctt cgagccgcgc gcccacctgc
     6721 tgcgcgtaca gatcgacggc aagccggcca agacagatga tgtcgcggct acgatccggc
     6781 gcgaaacggc tggctgcggc cgctgaagcg gacgcgggct cgatgccggt tgcgtttgcg
     6841 ttcacgatcc cggtcccgct ggatgtgctg gaatgatcca tgaatattcc tgaatagctg
     6901 agcgcgggcg tgaggcgcag taagcgccgg caacccgcgc gaatgaatca gatcgtcgtg
     6961 ccttcgagtt cgccgatcat cttctgcatc tcggcgccgc cggccatcat gtcgagcact
     7021 tcatccttgc tgatggtgtc cttggtgaac gtgccgagcg atttgccgcg attgagcagc
     7081 gtgaacgaat cgccgatcgg atacgcgtga tgcacgttgt gcgtgatgaa gatcaccgag
     7141 atgcctttcg cgcgcgccgt gtggatcagc ttcaacacgt tgaagctctg cttgacgccg
     7201 agcgcggcgg tcggctcgtc gagaatcagc acgcgcgcgc cgaagtgaat cgcccgcgca
     7261 atcgccagac attgcttctc gccgccggac atggtgccga tcggctgatg cgggtcgcgc
     7321 acgttgatgc ccatctccgc aagcttgtcg cgcgcggtgg tggcgctggt ttcgaggtcc
     7381 atcacgttca gaaagccgaa cagtttcttt tgcggctcgc ggcccatgaa gaagttgcgt
     7441 gcgaccgaga gcaacggcac cagcgccaga tcctgataga ccgtggcgat gccgagatcg
     7501 agcgcgtctt tcggcgattc gaacagcacg ggtttaccgt cgaccaggta ttgaccggaa
     7561 gagggttgat gcacaccggc caaggttttg atcagcgtcg atttgcccgc gccgttgtcg
     7621 ccgagcaggc aatgcacttc accgcgtttc aggcgcaagg tcacgccgct cagcgcgatg
     7681 acctggccga agtacttgct gacgtcttcg agcgcgagga tcacgtcgtc ctgcggctcg
     7741 ctcgtgccgg cagtattcac ggtgctcacg gtattcgtat cggacatggt cgtctcctcg
     7801 ttacgactgc gcgacgcggc ggcgcacgta gtgattgaac agcacggcga tcagcagcat
     7861 cacgccgagg aacacgcgga accagtcgga gtcgacattc gtataggtga tgccgatctg
     7921 caccacgccg aagatcagcg cgccgaagca ggcgccgatc accgagccgt agccgccggt
     7981 cagcagcgtg ccgccgatca ccgccgcgat gatggcttcg aattcggcct gcagcccgcg
     8041 gtcggccgct gccgacccga tgtcgcacac ttgcagcacg gcgaacaggc acgcgcagaa
     8101 cgcggtcagc acgaacagcg agatcttcac gcgccgcacc ggcacgccga cgttcttggc
     8161 tgcgttggcg tcgccgccga ccgcgaaaat ccagttgccg aagcgggtct tggcaagcgt
     8221 gaacgcgcag actgcggcca acgcgaacca ccagagcagg actttcggaa tgcccggcac
     8281 gagcggattg ccgttgtcga gcagttttac gacaccgagc tggccgagcc agatgaacag
     8341 gcccttgaac gcgacaccct ggaacagggt atgggcgagc cagtcgtgag cggcgacgtc
     8401 gcccacgccg gagatgatcg tgcggtcggc gaacatgacc gacagggcca gcgtgagccc
     8461 acgcaggatg aagaggaagg ccagcgtcac gataaacgac ggcagccgcg tgcgcatcac
     8521 cagatagccg ttgagtgcac cgagcagcat cgagccggcg aacgcaaaca ggatcgacaa
     8581 ggcaatcggc cagtggaagt acatggtggg cagcgcgacc atcatgcccg agaagccgat
     8641 catcgaaccg atcgacagat cgaattcacc ggcaatcatc aggaggcacg cgccgactgc
     8701 aatcaggccg agatacgcgg ccacttgcga ccagttcatc acgccgtcga ggttgaacat
     8761 gccggagttg ccggccgcaa tcccaaacac gaggaacacc atcacggtgc cggcgagggc
     8821 ggcgaattcc gggcggttca gcagatgccg gaaccacgat tcctggcgca cacgctcatc
     8881 ggcccccgcg gcgccggtgg cggaggcggg cgcactgttg ggcgtcgccg ccgttccggc
     8941 gccggagggg ggctgcttgc ggggatgggg atggaagggg ttggctacgc ccattgaagc
     9001 tctccttgat gcgcccgggg tggcagtcga cagcggattg caatctgacc gcatggcgcg
     9061 tgatgcacgt aaaacgtggt gaacacaggc gtggggcggc cagaccggtt gggaccggcc
     9121 gcatccactt acttcatacc cgccggaaca gttgtcccgt atcggcactt ccggcgaaat
     9181 gcgttagcga tactgcccgg cgtacttgac caccttgtcg atgttggcct tggtgatgaa
     9241 acccgggccg gaaccgatat tgcgcggccc gtaggcgggt gccaggccgt aggtggcgag
     9301 gcgttgcttg aacttcgggt tcgcttccag gatctggcgg atcttcgccg gatcggtggt
     9361 gtgttccttc ttcacgatgg ccagtacggc gaccgggata tagccttgca ggtagggttg
     9421 ctggtcgatt gcgaactgga tcgtgccgtc ctggatgccc ttcgcgatgt cattgtcgaa
     9481 atcgaatgtc gcgaaccaga ccttgccggc aaggcccatc tgctgcagcg ccttgatcgt
     9541 cggcgcggcc gacagcggac cgagcgtgag caccgcctga gtgttcggat gattgcgcag
     9601 atacgcgctc actttcgatt gcacaccggt cgggtcctgg ccggcgtcga gcgtggaaga
     9661 cttgaagtcg acgccgatcg cttcggcaaa gcctcggcag cgttcgaacg aagccgggtt
     9721 ggttgcgtaa tggttgacgc acaggaacga cttgatgccc gctgccttgg ctttttcgcc
     9781 cgccgccttg cccgcggtgt attcaggctg accgacgtgc atgattgcgc cgagttgcgc
     9841 gctttgcgct tccgtgccgg aattgatcgt gacgagcgga atgtgtttct cggtgacctt
     9901 ggcgattgca ctcttgagta cgtcgaagtc ggcgatcgag acgatcacgc catcgtagtt
     9961 acgggcggcc gcctgctcga tcagacgcgc catgtcggcg atgtcgccgt tcggcggatt
    10021 acggtaatcg gtctcgacgt tgaaatcttc gtcggcctgt ttgatggcgt tcttgatggt
    10081 gttccaccac gaatccgaat ccggcgcgtg gctgatc
//
LOCUS       DATASH010000589         2380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4649063,
            whole genome shotgun sequence.
ACCESSION   DATASH010000589 DATASH010000000
VERSION     DATASH010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4649063"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VHV99_15940"
     CDS             complement(<1..396)
                     /locus_tag="VHV99_15940"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX3636947.1"
                     /translation="MSDFILDNPFLEFLDARLVAWKSGYAEFRMPIQARNLNRQGVLQ
                     GGAVATLLDAACGYAGLYAGQGEPAIHGFTLSLTVNYLDTGLGESVTAKGFIERQGRS
                     IFFSRGEAWVDDKLLIASAQGTFKYAGTHE"
     gene            587..1549
                     /locus_tag="VHV99_15945"
     CDS             587..1549
                     /locus_tag="VHV99_15945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007736254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEX3636948.1"
                     /translation="MKRLYIQEVATRDGFQNEKQFVDTDAKIALVNALSACGFAKIEV
                     TSFTSPKAIPALRDAEAVMHGIARRDGVVYTVLVPNVRGAERALSCGVDEVNLVMSVS
                     ETHNRANLRMSRDQSFAQLSDVIGVVKATPVAINVSLSTSMGCPMEGDVAGEEVLRWT
                     GRFARLGVRGVTLCDTTGMAFPSQVKALCERVREAFPALELTLHFHNTRGMALANTLA
                     ALDAGIDRFDASLGGIGGCPYAPGASGNVCTEELVHMLELDGYDTGVDLTATLHAAAL
                     LPGLIGHDVQSQILKAGRRLDLHDAPPTDAGGAPVQQAFAKEGA"
     gene            1551..1934
                     /locus_tag="VHV99_15950"
     CDS             1551..1934
                     /locus_tag="VHV99_15950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017332064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3636949.1"
                     /translation="MAIIDHLDHLVLTCIDEAATRHFYTEVLQMKLETFGAGRTAFKF
                     GNQKINVHVRGKEFEPKAHVPVPGALDLCFIAAVPLDEVIAHLKQYEWPIIEGPVERT
                     GATQKIRSVYVRDPDLNLIEISELI"
     gene            2127..2270
                     /locus_tag="VHV99_15955"
     CDS             2127..2270
                     /locus_tag="VHV99_15955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636950.1"
                     /translation="MQTRIARGRARHRLHCFACTLRMTGMQHHAIPLRDKQPGGHLPE
                     STG"
BASE COUNT          498 a          745 c          705 g          432 t
ORIGIN      
        1 ctcgtgcgtg ccggcgtatt tgaatgtccc ttgcgcgctc gcaatcagga gtttgtcatc
       61 gacccatgct tctccacgcg agaaaaaaat gctgcgtcct tgacgctcga taaatccttt
      121 cgccgtcacg ctctcgccca accctgtgtc gagataattg accgtcagcg aaagcgtgaa
      181 accgtgaatg gccggttcgc cctgcccggc atagagacct gcataaccac atgcggcatc
      241 gagcagggtt gccaccgcgc cgccttgcaa cacgccttgc cgattcagat tacgcgcttg
      301 aatcggcatg cggaactcgg cgtaaccgct cttccacgca accaggcgtg cgtcgaggaa
      361 ttcgagaaac ggattgtcga gaatgaaatc ggacatcgaa aaatgctcct gccgtaaaag
      421 taggggtcac ccgagactgt gccacgacga agcatttggc gtgcgaagct gcccgggcga
      481 gcctttccaa tttggaaacg gtcgtggaaa atccactgat tttcaggccg tcgaggcagg
      541 ttcatcatgt ccgtataacg cgccaccatc cgacatgagg agccccatga aacgcctgta
      601 tatccaggaa gtcgcgaccc gagacggctt tcagaacgaa aagcaattcg tcgataccga
      661 tgcaaaaatc gctctggtca acgccctgag cgcgtgcgga ttcgcgaaga tcgaagtgac
      721 gtcgtttaca tcgccgaaag ccatccccgc gttgcgcgac gcagaagctg tgatgcatgg
      781 catcgcgcgg cgcgacggcg tcgtctacac ggtgctcgtg ccgaatgtgc gcggggcgga
      841 acgcgcgctc tcatgcggtg tcgatgaagt caacctcgtg atgtcggtga gcgagaccca
      901 caatcgcgcg aacctgcgca tgagccgcga ccagtcgttc gcacaattga gcgatgtgat
      961 tggcgtggtg aaggcaacgc cggtagccat caacgtgtcg ttgtccacgt cgatggggtg
     1021 ccccatggaa ggcgacgtcg cgggggaaga agtgctgcgc tggaccgggc gtttcgcccg
     1081 gctcggcgtg cggggcgtga ccctctgcga caccaccggc atggcgttcc cctcacaggt
     1141 gaaagcactc tgcgaacgcg tgcgcgaggc attccccgcc cttgaactca cgctgcactt
     1201 tcacaacacg cgcggcatgg cgctggccaa cacgctggcg gcgctggatg caggcatcga
     1261 tcgtttcgat gcatcgcttg gcggcattgg agggtgccct tatgcacccg gtgcgagcgg
     1321 caatgtatgt acggaggaac tagtccatat gctggaactc gacggctacg acactggcgt
     1381 cgatctcacc gcgacgctcc acgcagcggc gttgctgccg ggcctgatcg gccacgacgt
     1441 gcagagccag atcctcaaag ctggccgccg gctcgacctg cacgacgcac cgccgacaga
     1501 cgcaggtggc gcccccgtac aacaagcatt cgccaaggaa ggagcctgac atggccatca
     1561 tcgatcacct ggatcatctc gtcctgactt gcatcgacga agcggcgacg cgccatttct
     1621 acaccgaagt cctgcagatg aagctcgaaa ccttcggtgc aggacggacg gcgttcaagt
     1681 tcggcaacca gaaaatcaac gtccatgtgc gcggcaagga gttcgagccg aaggcgcatg
     1741 tgccggtgcc gggcgcgctc gacctgtgtt tcatcgcggc tgtgccgctg gacgaggtaa
     1801 tcgcgcacct gaagcagtat gagtggccaa tcattgaggg gccggtcgaa cgtaccggcg
     1861 ccacgcagaa aatccgctcg gtctatgtcc gcgatcccga tctgaatctg atcgagatct
     1921 ccgagttgat ttaaacaggg acgctttcgt tgttgtccgg aagccggcaa taagcgctgg
     1981 cgtccaacgc tcacaccgca ttcacgcgtt tgcggtgatc cgcatcgcct cgctgtccac
     2041 gaccgacgtg ttccctcacc gcaagcgctc cctcgctccg gcagccggat ctccggcaag
     2101 cgctcacacg cctcgaccag ccgatcttgc aaacccgtat cgcgcgcggc cgcgcgcggc
     2161 accgcttgca ttgcttcgcg tgcacgctca ggatgacggg catgcaacac cacgcgatac
     2221 cgctgcgcga caagcagccg ggcggccatt tgcccgagtc caccggctga cccggtaatg
     2281 aaaatgcgcg acagatggcg gcgtccttaa agatcagaca aatcaacacc agaccgatct
     2341 ctgtcgggta tgcgcgagag ccagcgcagc gcgccaagcc
//
LOCUS       DATASH010000590         4528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2076126,
            whole genome shotgun sequence.
ACCESSION   DATASH010000590 DATASH010000000
VERSION     DATASH010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4528
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2076126"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VHV99_15960"
     CDS             <1..285
                     /locus_tag="VHV99_15960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX3636951.1"
                     /translation="GVSHAALWGLAYVSLFSMLIGFVFWYRGLALGGIAGVGQLQLLQ
                     PFFGLLLAGLLLHEQVPPAMILVTVVVVGCVAGARRFSRTTPAAPAEPAA"
     gene            422..1309
                     /locus_tag="VHV99_15965"
     CDS             422..1309
                     /locus_tag="VHV99_15965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEX3636952.1"
                     /translation="MPTAFAQTVEARFAELTPTAKRIASYLLANLDRLGLETAHQIAQ
                     QAGTSGISVGRFLRSVGYQNLDDLKRELRGTQERPWMITDRLDAFRRVASEQGEAAQA
                     GAPTPSPALARSLELEIGAIQHVYQLAQTPTFARMAERISNADAVYILGIQSTRGISN
                     AFYSYLEYIRPRVFYSDGMSGSYVDSLNSEFASPYLIVTDTRAYSMIARRYCEAATRR
                     GLPFALVTDIYCPWARDFAGDLLQVKTDVGQFWDSLAPLTCLFNLLISAIVDRLGPTL
                     DERVARNRELQGELNQFDL"
     gene            1326..1889
                     /gene="ddpX"
                     /locus_tag="VHV99_15970"
     CDS             1326..1889
                     /gene="ddpX"
                     /locus_tag="VHV99_15970"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HEX3636953.1"
                     /translation="MNDQRLIEITPVTHSVDIDLVYATPRNLTGKPIYKAAHCLLLEP
                     AEAALRQAVQLARNIGTTLRIFDAYRPPQAQQVLWDFLPDPTYVADLSRGSNHSRGTA
                     LDLTLLDASGEELDMGTGFDAMTIESEHFHPGLPEHVQRNRTLLLGVMHGAGFTHIKS
                     EWWHYELPGSRVLPLIDNSESGPLRLM"
     gene            1949..3553
                     /locus_tag="VHV99_15975"
     CDS             1949..3553
                     /locus_tag="VHV99_15975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3636954.1"
                     /translation="MKLVLRNALAVAAVASALALSVAGGPVQAATPKDMFVMATLLDE
                     FTTLDPGEVYELVPEEYVANTYDRLVRVDLRDPSKFNGDVAQSWTVSPDGLTFTFKLR
                     PGLKFHSGNPLTADDVAWSIQRTVLLDKGPAAVLQGIGLAKDNVLQRVKKVDDSTVSV
                     TTDQKYAPTFVLNVLGSWPASVLDQKLLMSHQKGNDFGNDWLRTNEAGSGAYKLVKWT
                     ANDSIVLQRFDGYRLPLAMKRIVLRHVPEASSQRLLLENGDVDVARDLSPDDLATLGK
                     SGKARVTPVPQATLMYLGLNVKNPNLARPEVQEAMKWLIDYNGIQDHVVRSTYKVHET
                     FLPEGFLGALNTNPYQQNVAKARALLAKAGLANGFTVKMDVRNDYPYNEIAQAVQANL
                     AQANIKVEIIPGDNKQTLAKYRARQHDIYIGEWSADYIDPHSNAQGFTWNPDNSDASS
                     YKMLAWRNAWDIPELTKETNAALAESSTAKRAQMYQAMQKEVLAKSPFVIMFQKVAQV
                     AYRPGVSGLDVGPINDLVSYRDLKKQ"
     gene            3630..>4528
                     /locus_tag="VHV99_15980"
     CDS             3630..>4528
                     /locus_tag="VHV99_15980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011882391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3636955.1"
                     /translation="MSTPITPLTTLDRVRALCAGHGSVRGTLLLLRWVLTLLITFIGL
                     LAVTFIIGRKVPIDPVLAVLGDRASAQAYAAMRTELGLDKPLAVQFLIYVRDVLHGNL
                     GMSLLTANPVIDDIKRVFPATLELATLSTILGVLVGVPLGVIAAVRHNRWIDHVARFV
                     GLIGSSVPVFWLGLMGLLLFYAKLHWVSGPGRIDPVFDGMVDTNTGSLLIDSLIAGEW
                     EVFFNALSHIALPAAILGYYSVAYLSRMTRSFMLDQLSQEYITTARAKGLPERRVIWR
                     HAFGNIAVPLLTVIALAYSYLLEG"
BASE COUNT          841 a         1481 c         1329 g          877 t
ORIGIN      
        1 ggcgtgagcc acgcggcgct gtgggggctc gcctatgtgt cgctcttcag catgctgatc
       61 ggcttcgtgt tctggtaccg cggactggcg ctcggcggca ttgcgggggt cgggcaactg
      121 caactgctgc agccgttctt cggcttgttg ctcgccggct tgctgttgca cgagcaggtg
      181 ccgcccgcga tgattctggt taccgtcgtg gtggtgggat gcgtggcggg cgcgcggcgt
      241 ttctcgcgaa cgaccccggc ggcgccagca gagccagccg cgtaatagcc attgttcagc
      301 caaatcctat cgaccccagc ctcccgataa tttttttaag ctggcgctga gtttattggt
      361 tttgttagat acgtgtcatt gtttgagcag atgacaagcc cctaaccaaa acagcgcccg
      421 gatgccgact gctttcgccc aaaccgtcga agcccgcttc gccgaactca cgcccacagc
      481 caagcggatt gccagctatc tgctcgccaa tctcgaccgg ctgggtctcg agaccgcgca
      541 ccagatcgca cagcaggcgg gcaccagcgg catctcggtg ggccgcttcc tgcgcagcgt
      601 cggctatcag aatctcgacg atcttaaacg cgaactgcgc ggcacgcagg agcgcccgtg
      661 gatgatcacg gaccgcctcg acgcgttccg ccgcgtggcg agcgagcaag gcgaagccgc
      721 acaggccggc gcgcccaccc ccagccccgc cctcgcccgt tcgctcgagc tcgaaatcgg
      781 cgcgatccag cacgtctacc aactcgcgca aacgccgacg ttcgcgcgca tggccgaacg
      841 catttcgaac gcggacgccg tctatatcct cggcattcag tcgacccgcg ggatcagcaa
      901 cgcgttctat agctacctcg aatacatccg cccgcgggtt ttctattccg acggcatgtc
      961 gggctcgtac gtcgattcgc tgaactccga gttcgcgtcg ccgtatctga tcgtcaccga
     1021 cacgcgtgct tactcgatga tcgcgcgccg ctattgcgag gccgccacgc gacgcggcct
     1081 gcccttcgcg ctcgtcaccg atatctattg cccttgggca agagatttcg cgggcgatct
     1141 tctgcaggtc aagaccgacg tcggccagtt ctgggactcg ctcgccccgc tcacctgcct
     1201 cttcaacctg ttgatcagcg cgatcgtcga caggctcgga ccgacactcg acgaacgcgt
     1261 ggcccgcaat cgcgaactgc aaggcgaact caatcagttc gatctttgaa cctcacttca
     1321 tcgctatgaa cgaccaacga ctcatcgaaa ttacgccggt cacgcacagc gtggacatcg
     1381 acctcgtgta cgccacgccg cgcaatctca ccggcaagcc gatctacaag gcggcgcatt
     1441 gcctgctgct ggaacccgcc gaagccgcgc tgcgccaggc agtgcaactc gcgcgcaaca
     1501 tcggcacgac cctgcgtatt ttcgatgcct accgtccgcc gcaagcgcag caggtgctgt
     1561 gggatttcct gcccgaccca acctatgtcg ccgacctcag ccgcggttcg aaccatagcc
     1621 gcggcaccgc gctcgacttg acgctccttg atgcgagcgg tgaggaactc gacatgggca
     1681 cgggcttcga cgccatgacg atcgaatccg agcacttcca ccccggcttg cccgagcacg
     1741 tgcaacgcaa tcgcacgctg ctgctcggcg tgatgcatgg cgcaggcttt acacacatca
     1801 agagcgaatg gtggcactac gagctgccgg gctcgcgcgt gttgccgctg atcgacaaca
     1861 gcgaaagcgg tccgctgcgc ctgatgtaag ccgttcgtct ttgcagtgcc cctcttccct
     1921 ggctgttcac attcctcacg gagcgagtat gaaactggtt ttgcgcaatg cgttggcggt
     1981 ggccgccgtc gcgtctgccc tggcgctgtc ggtggcgggc ggtcccgttc aggcggccac
     2041 gccgaaagat atgttcgtca tggccacgct gctcgatgaa ttcaccacgc tcgacccagg
     2101 cgaagtctac gaactcgtgc cggaagaata tgtggcgaat acctacgacc gcctcgtgcg
     2161 tgtcgacttg cgcgatccgt cgaaattcaa cggcgacgtc gcgcaatcgt ggaccgtcag
     2221 ccccgacggc ctcaccttca cgttcaagct ccggccgggc ctcaagttcc attcgggcaa
     2281 tcctttgacg gccgacgacg tcgcctggtc gatccaacgc accgtgctgc tcgataaagg
     2341 tccggcggcg gttctgcagg gcatcggtct ggcgaaagac aacgtgctgc agcgcgtgaa
     2401 gaaagtcgac gacagtaccg tgagcgtcac gaccgaccag aaatacgcac cgactttcgt
     2461 gctcaacgtg ctgggttcct ggccggcttc ggtgctcgac cagaagctgc tgatgtcgca
     2521 tcagaagggc aacgatttcg gcaacgactg gctgcgcacc aacgaggccg gctccggcgc
     2581 ctacaaactc gtcaagtgga ccgccaacga cagcatcgtg ctgcaacgct tcgacggcta
     2641 ccgcttgccg ctcgcgatga aacgcattgt gttgcgccat gtgccggaag cgtcgagcca
     2701 acgtcttctg ctggaaaatg gcgacgtcga cgtcgcgcgc gatctgagcc ccgacgacct
     2761 cgccacgctc ggcaaaagcg gcaaagcccg cgtgacgccg gtgccgcaag ccacgctgat
     2821 gtacctcggc ctgaacgtga agaacccgaa cctcgccagg cccgaggtgc aggaagcgat
     2881 gaaatggctg atcgactaca acggcattca ggatcacgtc gttcgatcca cctacaaggt
     2941 gcacgagacc ttcctgccgg agggcttcct tggcgcgctg aatacgaacc cgtatcagca
     3001 gaacgtcgcc aaagcccggg cgttgctcgc gaaagccgga ctcgccaacg gcttcaccgt
     3061 gaagatggac gtgcgtaacg actacccgta caacgaaatc gcccaggcgg tacaggccaa
     3121 tctcgcgcag gccaacatca aggtcgagat cattccgggc gacaacaaac agacgctcgc
     3181 aaaatatcgc gcccgtcagc acgatatcta tatcggtgaa tggtccgcgg actatatcga
     3241 cccgcacagc aacgctcagg gcttcacgtg gaatccggac aatagcgacg cgtcgagcta
     3301 caagatgctc gcctggcgca atgcgtggga cattcccgag ctgaccaagg aaaccaacgc
     3361 ggcgctggct gaatcgtcga ccgccaaacg cgcgcagatg tatcaggcga tgcagaagga
     3421 agtgctggcg aagtcgccgt tcgtcatcat gttccagaaa gtcgcgcagg tggcgtatcg
     3481 tccgggcgtg agcggtctcg atgtcggccc gatcaacgac ctcgtgtcgt atcgtgatct
     3541 gaagaagcag taagcgtcag agcctgaggc gcggtgtttt tgcggcgatc gtcgcgtcat
     3601 ggccgcgctt caggcaagga aagataacca tgtcgactcc catcacgccc ctcaccacgc
     3661 tggaccgcgt gcgcgcgctt tgcgccggtc acggcagcgt acgcgggacc ctgcttctgc
     3721 tgcgctgggt gcttacgctg ctgatcacgt ttatcggcct gctcgcggtg accttcatca
     3781 tcggccgcaa ggtgccgatc gatccggttc tggccgtgct cggcgatcgc gcttccgcgc
     3841 aggcttatgc cgcgatgcgc accgaactcg gcctcgacaa accgctcgcg gtgcaatttt
     3901 tgatctatgt acgcgatgtg ttgcacggca atctcggcat gtcgctgctg acagcgaatc
     3961 cggtcatcga cgatatcaag cgcgtcttcc ccgccacgct cgaactggcc acgctatcca
     4021 cgattctcgg cgtgctggtg ggtgtgccgc tcggcgtgat cgccgcggtc cgccataacc
     4081 gctggatcga tcatgtcgcg cgcttcgtcg gtctcatcgg cagttcggtg ccggtgttct
     4141 ggctcggtct gatgggcttg ctgctgttct acgcgaagct gcattgggtg tccggtcccg
     4201 gccgcatcga tccggtgttc gacggtatgg tcgataccaa taccggcagt ctgctgatcg
     4261 actcgctgat cgccggcgaa tgggaggtgt ttttcaacgc gctctcgcat atcgccttgc
     4321 ccgcggcgat tctcggctat tactccgtcg cctatctgag ccggatgacg cgctccttca
     4381 tgctcgacca gttgagtcag gaatacatca ccacggcgcg tgccaaaggc ttgcccgagc
     4441 gccgcgtgat ctggcggcac gcgttcggca atatcgccgt gccgcttctt accgtgatcg
     4501 cactcgccta cagctatctg ctcgaagg
//
LOCUS       DATASH010000591        14144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6031343,
            whole genome shotgun sequence.
ACCESSION   DATASH010000591 DATASH010000000
VERSION     DATASH010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 14144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14144
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6031343"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(190..1728)
                     /locus_tag="VHV99_15985"
     CDS             complement(190..1728)
                     /locus_tag="VHV99_15985"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX3636956.1"
                     /translation="MSETYQDETVRSETSARALTHFIDGKTIDGTSGRFGDVFNPALG
                     SVSARVPLASVAEVDAAVAAASAAFPAWSETAPIKRARVLFKFKELLDRHHDELAELI
                     TREHGKVFSDAKGEVMRGIEIVEFACGIPNLLKTDFTDQIGGGIDNWNLRQSLGVVAG
                     ITPFNFPMMVPCWMFPVAIACGNTFVLKPSERDPSVSNRLAELLKEAGLPDGVFNVVH
                     GDKVAVDALLVHPEVSALSFVGSTPIAEYIYTEGTRHGKRVQALGGAKNHLVVMPDAD
                     LNQAVDALIGAAYGSAGERCMAISVAVAVGHIADELIERLTPRVKSLKILNGMESEAE
                     MGPLVTAAHREKVTGYIDAGVAAGAKLVVDGRGHQVAGHEKGFFLGGTLFDDVSTDMK
                     IYREEIFGPVLCVVRVPDFASAVELINANEFANGVSLFTSDGGVARAFARRIEIGMVG
                     INVPIPVPMAWHSFGGWKRSLFGDHHAYGEEGVRFYTRYKSIMQRWPDSIGKGAEFTM
                     PVAK"
     gene            1859..2758
                     /locus_tag="VHV99_15990"
     CDS             1859..2758
                     /locus_tag="VHV99_15990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3636957.1"
                     /translation="MDLTLLRAFVTVAREGNLTRAAVQLHLTQPAVSLQIKHLQETLG
                     VTLFTRTSHGLSLTRDGQALLPHAERALGAAGDVQRAAAALRHEVRGRLRIGTILDPA
                     FLRLGGFLKQLVETWPHIETALRHGMSGWVLEQVRAGELDVGYYIGLPSDDDSRDGAA
                     FHAVTLTHFQYRVLAPAGWKDRVKGARDWRSLAALPWIWTPPASAHNRLLSRCFGEAG
                     VKPVKVAEVDQEPSMLDLVKSGVGLTLARDATAIAEAHAHALTIVEGITVPTQLSFIT
                     LTERKDEPAIAAALKLIEQQWAT"
     gene            3217..3753
                     /locus_tag="VHV99_15995"
     CDS             3217..3753
                     /locus_tag="VHV99_15995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HEX3636958.1"
                     /translation="MARNIEIKARAQHFEQLRERAAKLAPDAPLIFRQQDFFYDVPRG
                     RLKLRQFDDGTPAELIFYQRDDRDGPKASYYTRSPVTNPEAMHALLATALTTRGIVTK
                     ERHVYLTGRTRIHLDRVDGLGDFVELEVLLAQDDDEEGGHAEAHAMFQTLGVPESDLV
                     AVAYVDLLSPDSEPQQAV"
     gene            complement(3960..4451)
                     /locus_tag="VHV99_16000"
     CDS             complement(3960..4451)
                     /locus_tag="VHV99_16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX3636959.1"
                     /translation="MLELDHFDLALLDVLQRFGRATHQQLAEQVPLSPSQIGRRLQRL
                     EQVGVVDGYRVVLRPEKLGLGVTAFTSLKLKHHGDSIIEQFQQQIDVLPEVLECHAVV
                     GDADYLLRIVAPDLNYLSTFVMKKLMRVPGVDSVRSNIVLTTFKRNGPLPLGHLSPGA
                     AVA"
     gene            4600..5490
                     /gene="phhA"
                     /locus_tag="VHV99_16005"
     CDS             4600..5490
                     /gene="phhA"
                     /locus_tag="VHV99_16005"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004505 - phenylalanine 4-monooxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HEX3636960.1"
                     /translation="MSTANTAKLKEQFDAGLETRADFTIDQPTERYGAVDHAVWQQLY
                     ARQTALLKGRVCDEFLAGVDCIGMPSDRVPSFDEINAKLTPATGWQIVAVPGLVPDQV
                     FFEHLANRRFPVTWWMRRPDQLDYLQEPDCFHDLFGHVPLLINPVFADYMHAYGRAAL
                     AANDAGALPLLARLYWYTVEFGLIRDPASPNGVKIYGAGIVSSKGETLYSQHSAAPNR
                     LGFDLGRVMRTRYRIDTFQKTYFVIDDFAQLFRVAQTEFAPLLAKLTAQPAFAAGDVL
                     ECDHIITRGSQEGWQADGDV"
     gene            5539..5883
                     /locus_tag="VHV99_16010"
     CDS             5539..5883
                     /locus_tag="VHV99_16010"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004533512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEX3636961.1"
                     /translation="MADAHVHWRARSDQRGSTMISRLTPEERTTQVAELHGWQAVAGR
                     DAIQRHFKFADFNEAFGFMTRVAIKAQEMDHHPEWFNVYNKVEITLSTHEANGLTERD
                     IKLARFIDSITV"
     gene            complement(5977..6201)
                     /locus_tag="VHV99_16015"
     CDS             complement(5977..6201)
                     /locus_tag="VHV99_16015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3717 domain-containing protein"
                     /protein_id="HEX3636962.1"
                     /translation="MSEITIQELEAAINFWRARSPSSGDELVLCKEASALSKPYALLI
                     VQRQQSLPPDRLDRFARQAWDSYVRLNNSL"
     gene            6375..7061
                     /locus_tag="VHV99_16020"
     CDS             6375..7061
                     /locus_tag="VHV99_16020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3636963.1"
                     /translation="MRLLLIEDDRPIARGIQSSLEQAGFTVDMVHDGIFAEQALTQNR
                     HELVILDLGLPGIDGMTLLARFRQSNRHTPVIILTARDELNDRVQGLNSGADDYMLKP
                     FEPTELEARIRAVMRRSGPHGDMPRPEVSLGGVRLSGVDRRIFNDDKPLELSPREFAV
                     LEMLLLRHGRVVSKAQLQDHLTHFGGDLGDTAIEVYVHRVRKKLESCRVEIVTVRGFG
                     YLLQEIRQAA"
     gene            7214..8716
                     /locus_tag="VHV99_16025"
     CDS             7214..8716
                     /locus_tag="VHV99_16025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018432433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HEX3636964.1"
                     /translation="MPQPAANSLRRTLLRRLAAPLSLLALMSGLIAYWLAWQYTQHVV
                     DRSLADLATAISKQIQIAGPNAKVTVPPLAQAMFSDPVEQLVYRISNGETEIAGDHNL
                     PLQGTSVRRMHYAYVFETQHEGVTVRVAQVRVDQPAGNPIVVEVGQPVHHRYRIAAEF
                     LVAIMMPLLLLLLAGWVIVWRVVNQQLNPLTDLADSLNRQTHTSLEPVDETYVPVEIR
                     PLTGALNALLGRLKTALDGQRKFIADAAHQLRTPLTAVKLHAEQAAVARDPQQTLAAV
                     RELRAAADRAVRLSNQLLSLARAEPGEQAARFVNVDMAALAFDTGAEWVPRALTFHVD
                     LGFQRLDDPTNDHPLMARGNPVLLHEVIANLLDNALKYVPPSRFDGGRITVTVSQTVI
                     DDLRMAEIVVEDNGPGVPPTQQADLFKRFFRGDGQSDAGVDGGAGLGLAIVHDIMVLH
                     RGSVHYEDAPEGGARFIVRIPLIPISVQPNVAPETRRPAKKTAHSAPIDM"
     gene            complement(8790..9305)
                     /locus_tag="VHV99_16030"
     CDS             complement(8790..9305)
                     /locus_tag="VHV99_16030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HEX3636965.1"
                     /translation="MSSRRIAVRRSGVHGKGVFAIEPIAAGERLIEYKGERISWKEAL
                     RRHPHNPDEPNHTFYFALDSGKVIDGKVDGNSARWINHSCAPNCEAEEIDGHVYVHAL
                     RDIAEGEEVFYDYGLVIDARQTKKLKKEYECRCGARKCRGTMLAPPEKEKGASRSDKA
                     VKKPAKKAKKK"
     gene            complement(9527..10639)
                     /locus_tag="VHV99_16035"
     CDS             complement(9527..10639)
                     /locus_tag="VHV99_16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010093147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HEX3636966.1"
                     /translation="MSALFSPLTLRSVTLPNRIVVSPMCQYSAERGEATAWHMIHLGS
                     LALSGAGMLCIEATAVEPDGRITPGDLGLWDDATEAALEPVLAAIRKHSHIRVAMQLG
                     HAGRKASSHAPWDGGQLIPVSQGGWLPHAPSALPHKAGEEPPLALDTPALNRIREAFA
                     ASARRAARLGIDALEVHAAHGYLLHQFLSPIANQRSDDYGGSLENRMRFPLEIFDIVR
                     AAFPADKPVGVRVSATDWVEGGWTLEDTIAFAQELKKRGCDWIDVSSGGVSPLQKIPL
                     EPGYQIPFARAVKQATGLTTIGVGLITDPLHAEQIIEEGDADLIAMARALLYNPRWPW
                     HAAAQLGATVEAPPQYWRSQPREQKALFGDISFGQR"
     gene            complement(10711..11424)
                     /locus_tag="VHV99_16040"
     CDS             complement(10711..11424)
                     /locus_tag="VHV99_16040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEX3636967.1"
                     /translation="MNREVLAIRHVHFEDLGSLELELGERGRPVRYLDVGVGRIEAPD
                     PVAASLMVVLGGPISATDDALYPTLVPLLAMIEKRIAAGLPTLGICLGAQLIARVLGA
                     RVYPAGHTELGWTPLTLTDAGRASPLRHLDGAHTSMLHWHGDTFDLPDNATRLASTPA
                     CENQAFAWGGHVLGLQCHPEIRADRFEPWLIGNAGEIAGHGIDARQLRAETAQFGPAL
                     ETAARRMFSEWLDQVEPKL"
     gene            complement(11552..12190)
                     /locus_tag="VHV99_16045"
     CDS             complement(11552..12190)
                     /locus_tag="VHV99_16045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX3636968.1"
                     /translation="MSEGVYGEQAAGRVTHSLLRLSTAMRSQAWEWAEGAGLTPTQGE
                     ILVLLMQRKGPMRLGEIARETALTAATTSDAVSTLETKGLVEKRRALDDGRALAVRLT
                     ARGRTAAKRAAQWPDFLAKAVGTLRDEEQTLFYRTLLKTIHQLEAQGTIPPHRMCLSC
                     THFEPSKNPKKTPHHCALLDMNMSDTDLRLDCSVYETADVATQKKTWKIFAQ"
     gene            complement(12728..13843)
                     /locus_tag="VHV99_16050"
     CDS             complement(12728..13843)
                     /locus_tag="VHV99_16050"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HEX3636969.1"
                     /translation="MSLEPFIDSKPFTFGVELEMQIVNTHDYDLTKAGSDLMRLIKDE
                     KIPGNITPEITESMIELSTGICTTHEQAVTDLRKIRDTLVSAADRLNVGLCGGGTHAF
                     QQWSERQIVDTPRFQYISELYGYLAKQFTVFGQHVHIGCPNPNSALYLLHSMSRFIPH
                     FIALSASSPFVQGVDTGFHSARLNSVFAFPLSGRAPFVLTWDSFEEYFSKMVHTGVVN
                     SMKDFYWDIRPKPGFGTIEVRVMDTPFSVDRAAAIACYIQTLARHLLLDKPITPKEDD
                     YLVYTFNRFEACRFGLAGNCIDPQTSERKTISEDILETLDLIAPHAEALGSGNALAEI
                     GAIARGQVNDATWLRGVFEQEKSLNEAVRQQCLQWRA"
     gene            complement(13973..>14144)
                     /locus_tag="VHV99_16055"
     CDS             complement(13973..>14144)
                     /locus_tag="VHV99_16055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium:proton exchanger"
                     /protein_id="HEX3636970.1"
                     /translation="PMSALAYLLVDDTYNLYPNFDPQLRAIVMCSIFVLQILGPWLVY
                     RSLAMVAERREE"
BASE COUNT         2838 a         4517 c         4074 g         2715 t
ORIGIN      
        1 gctggcgggg tgagccgctt tctttgctta tctttctttg cggcggcaaa gaaagtaagt
       61 gccgccccgc acaggggcaa cgctaataga ccaataacaa ttcaaggaaa ggccaaagag
      121 tcagatcaag gaaaggccaa cgccgtaggc aaccaaccac aaaagcaccg cgcacgcaaa
      181 cccaaaccct tatttagcca ccggcatagt gaactcagcg cccttgccga tactatcggg
      241 ccacctctgc atgatgctct tataccgcgt ataaaaccgc acaccctctt ccccataggc
      301 atggtgatca ccaaacagag acctcttcca cccaccaaaa gaatgccatg ccatcggcac
      361 aggaatcggc acattaatcc ccaccatccc aatctcgatc cgccgcgcaa aagcccgagc
      421 cacaccacca tcggaggtaa acaacgacac cccattggca aactcgttag cgttaatcaa
      481 ctcgacagca gacgcaaaat ccggcacccg cacaacacac agcaccggac caaaaatctc
      541 ctcgcgataa atcttcatat cagtcgagac atcatcgaac aacgtgccgc cgagaaagaa
      601 tcctttctca tgcccggcca cctgatggcc ccgtccatcc acaaccagct tcgcacccgc
      661 cgcaacaccg gcatcgatat aaccagtcac cttctcgcga tgcgccgccg tcaccaacgg
      721 ccccatctcg gcttccgatt ccatgccgtt gagaatcttc aggctcttca ctcgcggtgt
      781 cagtcgctcg atcagttcat cagcaatatg ccccactgcg acagccaccg aaatcgccat
      841 gcaacgctcg cccgccgaac cgtaggccgc accgatcagc gcatcgacag cctgattgag
      901 atccgcgtcc ggcatcacca cgaggtgatt cttcgcaccg cccaatgcct gcacgcgctt
      961 gccgtgccgc gtgccttccg tgtagatgta ctcggcaatc ggcgtcgaac cgacaaacga
     1021 caacgcgctc acctcgggat gcacgagcaa cgcatccacc gcgaccttgt cgccatgcac
     1081 gacgttgaac acgccatccg gcaaacccgc ctctttcagc agttccgcca atcgattcga
     1141 gacggacgga tcacgctccg acggcttcag cacgaacgtg ttgccgcatg cgatcgccac
     1201 tgggaacatc cagcacggca ccatcatcgg gaaattgaat ggcgtaatcc cggcgaccac
     1261 gcccagcgac tgacgcaaat tccagttgtc gatgccgccg ccgatctggt cggtgaagtc
     1321 ggtcttcaac agattcggaa tgccgcacgc gaactcgacg atctcgatgc cgcgcatcac
     1381 ctcgcccttc gcatccgaaa ataccttgcc gtgctcgcgc gtgatcaact ccgcgagttc
     1441 gtcatggtga cggtcgagca gttccttgaa cttgaacagc acgcgcgccc gcttgatcgg
     1501 cgcggtttcg ctccatgcag gaaacgctgc actggcggca gcgaccgccg catctacttc
     1561 cgcgacgctc gccagcggca cgcgcgcgct cacgctgcca agcgcggggt tgaagacgtc
     1621 accaaaacgg ccgctcgtgc cgtcgatcgt cttgccgtcg atgaaatggg tcagcgcgcg
     1681 tgcgctcgtc tcgctccgga cggtctcgtc ctgataggtc tcgctcatct tgtcctcttc
     1741 ctttgaatgt ccttcgcgca ccgcgaaccg cgtgatgaac gcaagcgtac ggcgcttggg
     1801 ggcgacaaat ccaatgagta atgctcaact gtgctataag ccgagctaat atcaagatat
     1861 ggatctgact ctgctacggg cgttcgtcac cgtcgcacgc gagggcaacc tcacgcgtgc
     1921 cgcggtgcag ctccacctga cccaacctgc cgtcagtctg caaatcaagc acttgcagga
     1981 gacgctcggc gtgacgctgt tcacgcggac ctcgcacggg ctttcgctga cacgcgatgg
     2041 tcaggccctg ttgcctcacg ccgaacgcgc gctgggcgcc gcgggcgacg tgcagcgcgc
     2101 ggcggcggcg ctgcgtcacg aagtacgcgg tcgattgcgg atcggcacga ttctcgaccc
     2161 cgctttcctg cgcctcggcg gctttctcaa gcaactggtg gaaacctggc cgcacatcga
     2221 aacggccttg cggcacggca tgtcgggttg ggtactggag caggttcgcg cgggagaact
     2281 ggacgtgggc tattacatcg gcctgccatc ggatgacgat agccgcgacg gcgctgcctt
     2341 tcacgccgtc acgctcacgc actttcagta ccgcgtgctg gcgccggcgg gctggaaaga
     2401 tcgtgtgaaa ggcgcgcgcg actggcgctc gctcgctgcg ctgccgtgga tctggacgcc
     2461 acccgcatcc gcgcacaaca gattgttgtc gcgctgcttt ggcgaggccg gcgtaaagcc
     2521 ggtcaaggtg gcggaagtgg atcaggagcc gtcaatgctc gatctggtca aatcaggcgt
     2581 cgggctaacg ctcgcacgcg acgccaccgc gatcgccgaa gctcacgcgc acgcgttgac
     2641 catcgtcgaa ggcatcactg tgccgaccca gctcagcttc atcacgctga cggagcgcaa
     2701 ggacgaaccg gcaattgccg ccgcgctgaa actgatcgag cagcagtggg caacttgagt
     2761 gcggcatgaa ggcgcacctg aggacaaaac gagagcgata tgagccactc tcatgacagc
     2821 tgcgtactgt ttaggccgga cgatgatggg tactctcgcc ggctgcccca ttagggcgac
     2881 cttcctcgcc gttcctgcgg gccgccgtcg ggttcggggt tcgggctcgt ggtccgctgt
     2941 tggccgctgc tggctggtgc tggtgctgtt cgcgcttcgc ggtccgccgt ccgcttgtgc
     3001 tcagtgccag tcgctctcgc cattcgcgtt gacaaaccat cctcatcgat cccgcatccg
     3061 aaaccacacc gtcacgcgct ttttgttaga ttgggggttc gcgctcacgc gccgaccccg
     3121 catcgttttg ttccgtctga cgttgttctt cctggcagct tcgccccggc aacatcggaa
     3181 gccgccgacc acgccatcct tcgacaggag cctgacatgg cacgaaacat tgaaatcaaa
     3241 gcccgcgccc agcatttcga gcaactgcgc gaacgtgccg cgaagctcgc gcccgacgcc
     3301 ccgctgatct tccgccagca ggactttttc tacgatgtac cgcgcggccg cctgaagctg
     3361 cgtcagttcg acgatggcac gccggccgaa ctgatcttct atcagcgcga cgatcgcgac
     3421 ggcccgaaag cgtcgtacta cacgcgcagc ccggtgacca atcctgaagc gatgcacgcg
     3481 ctgctcgcca cggcgctgac tacgcgtggc attgtcacga aggaacgcca cgtttatcta
     3541 acgggccgta cgcggattca tctggaccgc gtcgatggtc tcggcgactt cgtcgagctt
     3601 gaagtcctgc tggctcagga cgatgacgaa gaaggcggcc acgccgaagc acacgcgatg
     3661 ttccagaccc tcggcgtgcc ggaatcggat ctggtggcgg tggcctacgt cgatctgctg
     3721 agtccggaca gtgagccgca gcaggctgtg taaaacttag acacgcgggc cgcggacgaa
     3781 atcagttcgg gaacagcggc ctgaaaagac ctcgcggtct cagtgatctc agcggtgcca
     3841 gcggtctcag ccgtcccagc ggtcccagcg gtcccagccg tcccagcggt cttagccgtc
     3901 ccagcggtcc cagctgtcct agcgatttca gttgcctgaa cgcccggaaa accgccgcat
     3961 caagcgacgg ccgcccccgg cgacaggtgg ccgagcggca acggtccatt gcgcttgaac
     4021 gtggtcaaca cgatattgga gcgcacgctg tcgacccccg gcacacgcat caacttcttc
     4081 atcacgaacg tcgagagata gttcagatcc ggggccacga tgcgcaacag ataatcggcg
     4141 tcgccgacca ctgcgtgaca ttccagtact tcgggcagca cgtcgatctg ttgctggaat
     4201 tgctcgatga tcgagtcgcc atgatgcttc aacttcagac tcgtaaaagc cgttacgccg
     4261 agcccgagtt tttccggccg cagcacgacg cgataaccgt ccaccacacc cacctgctcc
     4321 aaccgctgca agcgccgccc aatctgcgac ggtgacagcg gcacctgctc agccaactgc
     4381 tgatgcgtag cccggccaaa gcgctgcaga acgtccagaa gcgcgagatc gaaatgatcg
     4441 agttccaaca tgattgatat ccgcattaaa tccgttgtct atgcacgatt attgcatatt
     4501 gaagcgtcat aacgccatca ttgcgccctt tccgcatgct cgccgctcta cacttgcatc
     4561 accgagtcaa cccgcagtta tcgtagagcg cgcagcatta tgtccacagc caacaccgcc
     4621 aaactcaagg agcaattcga cgccggcctc gaaacccgcg ccgatttcac catcgaccag
     4681 ccgaccgaac gctatggcgc agtcgaccat gctgtctggc aacagcttta cgcacgccag
     4741 accgcgttgc tcaaaggccg tgtgtgcgat gagtttctgg caggcgtcga ctgcatcggc
     4801 atgccgtccg accgcgtgcc ctcgttcgac gagatcaacg cgaagctgac ccccgcgacc
     4861 ggctggcaaa tcgtcgccgt gcctgggctc gtgcccgacc aggtattctt cgagcatctg
     4921 gcgaaccgcc gctttcccgt gacctggtgg atgcgccgcc ccgaccagct cgattatctg
     4981 caggaaccgg actgttttca cgacctgttc ggtcacgtgc cgctgctgat caatccggtg
     5041 ttcgcggact acatgcacgc gtacggccgc gcggcgctcg ccgccaatga cgccggcgcc
     5101 ctgccgctac tcgcgcggct ctattggtac acggtcgagt tcggcctgat ccgcgacccg
     5161 gcgagtccga acggcgtgaa gatctacggc gcgggcatcg tgtcgagcaa gggcgagacg
     5221 ctctacagcc agcacagcgc ggcgccgaac cggctcggct ttgacctcgg acgcgtgatg
     5281 cgcacgcgct accgcatcga cacgttccag aaaacctact tcgtgatcga cgacttcgcg
     5341 caattgttcc gcgttgcaca aaccgagttc gcgccgttgc tcgcaaaact gacagcgcaa
     5401 ccggcgttcg cggcgggcga tgtgctcgag tgcgatcaca tcatcacgcg cggctcacag
     5461 gaaggctggc aagccgacgg cgatgtctga cgatcaaact gatcgggccg acagcaggct
     5521 gatggcatcc gtgaaaaaat agcggatgcg cacgtccact ggcgtgcccg atcagatcaa
     5581 cgaggtagca cgatgatttc cagactgaca cccgaagaac gcaccacgca agttgcggaa
     5641 ctgcacggct ggcaagccgt agcaggccgc gacgcgatcc agcgccactt caaattcgcc
     5701 gacttcaacg aagcattcgg cttcatgacg cgcgtggcga tcaaggccca ggagatggac
     5761 catcacccgg aatggttcaa cgtgtacaac aaggtggaaa ttacgctgtc cacgcacgag
     5821 gccaatggtt tgaccgaacg ggacatcaaa ttagcacgat ttatcgacag tattaccgtg
     5881 taatacaagc aatcggcgca tggatgcgcg ttgcgtcgaa caattagcgt tcgatgaaag
     5941 ggcattgcag gcaatcccgc gcccaaacct tcagttctac aggctattat taaggcgaac
     6001 gtaagaatcc cacgcttgtc tggcgaaccg gtccaaacga tccggtggta gcgactgttg
     6061 acgctgtaca atcaacagcg catacggctt ggagagcgcg cttgcctcct tgcacagaac
     6121 gagttcgtcg ccgctcgaag gtgagcgggc gcgccagaag ttgatcgcgg cttccagttc
     6181 ctgaatcgtt atctcggaca tgacgtcgtg atcgttcggt ggctgcctga tggctacttc
     6241 atggcggctc gatagctgca tcgtgggcgc tttgtgccag ttcggcacgg ccacgcggca
     6301 cgctgcctgt gcagcaggcg ccccggcgaa ccgcttgccc aaacgcctaa aaccattgta
     6361 cttgagtgaa atccatgcga ctccttctga tcgaagatga ccgccccatc gcacgcggca
     6421 tccaaagcag tctcgaacaa gccggcttca ccgtcgacat ggtccatgac ggcattttcg
     6481 ccgaacaggc cctcacccaa aaccgtcacg aactggtgat cctcgatctg gggctgcccg
     6541 gtatcgacgg catgacgctg cttgcacgct tccgtcagag caatcgtcac acgccggtga
     6601 tcatcctcac cgcgcgcgac gagttgaacg accgtgtgca aggcctgaat tccggcgcgg
     6661 acgactacat gctgaaaccg ttcgaaccca ccgaactcga agcgcgcatc cgcgccgtga
     6721 tgcgccgcag cggcccgcac ggcgacatgc cgcgtccgga agtgtcgttg ggcggtgtgc
     6781 gtctgtcggg cgtcgatcgc cgcattttca acgacgacaa gccgctcgaa ctgtcgccgc
     6841 gcgaattcgc agtgctcgaa atgctgctgc tgcgccacgg ccgcgtggtc agcaaggctc
     6901 agctgcaaga ccatctgaca cacttcggcg gcgatctcgg cgataccgcg atcgaagtct
     6961 atgtgcaccg ggtgcgtaaa aagctcgaaa gctgccgtgt cgagatcgtc acggtgcgtg
     7021 gcttcggtta tctgttgcag gaaattcgcc aggccgcata atcgtcacaa gggccgcaca
     7081 ccgcgctgtt gtattgctgt gtgttgcggc ctgacggcgc cagaccggcg ccgcgcacct
     7141 ggtgcgcact tcgccggcat ccggcggatt tcacggcgct tgtcgccgtt gcctctttcc
     7201 cgcgcgttcg atcatgcccc agccggccgc caatagtctg cgccgcacgc tgctgcggcg
     7261 cctcgctgct cctctttcgc tgctcgcgct gatgagcggc ctgatcgctt actggctggc
     7321 gtggcagtac acgcagcacg tggtcgaccg gtcgctcgcg gatctcgcca ccgcgatctc
     7381 caaacagatc cagattgccg gccctaacgc caaggtgacg gtgccgccgc tcgcgcaggc
     7441 catgttctct gatccggtcg aacaactggt ctatcgaatc agcaacggtg aaaccgaaat
     7501 tgccggcgat cacaatctgc cgctgcaagg caccagcgtg cgccgcatgc attacgcgta
     7561 cgtcttcgaa acccaacacg aaggcgtgac cgtgcgcgtt gcacaggtgc gcgtcgatca
     7621 gcccgccggc aatccaatcg tcgtcgaagt gggtcagccg gtgcatcacc gttatcggat
     7681 cgccgccgag tttctggtcg cgatcatgat gccgctgctg ttgttgctgc tggcgggctg
     7741 ggtgatcgtg tggcgtgtcg tcaatcaaca gctcaatccg ctgaccgatc tcgctgactc
     7801 gctgaaccgc cagacccaca cgtcgctcga accggtggac gaaacctatg tgcctgttga
     7861 aatccggccg ctcacgggcg cgctgaatgc gctgctcggg cgcctgaaaa ccgccctcga
     7921 cggccagcgc aaatttatcg ccgacgcagc gcatcagctg cgcacgccgc tcaccgccgt
     7981 caagctgcat gcggaacagg ccgcggttgc acgcgacccg cagcaaaccc tcgccgcggt
     8041 ccgtgagcta cgcgcagccg ccgaccgcgc cgtgcggctg tcgaaccagt tgctttcact
     8101 cgcgcgcgct gagccgggcg aacaggcggc ccggttcgtc aatgtggata tggccgcact
     8161 tgcgttcgac acgggtgctg aatgggtgcc ccgcgcattg acgtttcatg tcgacctggg
     8221 ttttcagcgc cttgacgatc cgaccaacga tcaccctctg atggcgcgcg gcaatccggt
     8281 gctgctgcat gaagtgatcg ccaatctgct ggataacgcg ctcaagtacg tgccgccttc
     8341 gcgcttcgat ggcgggcgca tcaccgtgac ggtgtcgcaa accgtgatcg acgatctgcg
     8401 catggccgag atcgtcgtcg aagacaacgg accgggcgtg ccgcccacgc agcaagccga
     8461 tctgttcaaa cgattcttcc gtggcgacgg tcagagcgac gccggtgtgg atggcggtgc
     8521 cggcctcggt ctcgcgatcg ttcacgacat catggtgctg catcgcggca gcgtgcatta
     8581 cgaagatgcg ccggaaggcg gggcgcgctt tatcgtgcgt attccgctta taccgatcag
     8641 tgttcagcct aacgtcgctc ctgagacacg gcgcccggca aaaaaaacgg cgcactcggc
     8701 gccgatcgac atgtaactct gcagcggctc acaacgcgct gccacgggct gccttgagtt
     8761 cgattacgcc aacccaggca gcccgcattt catttcttct tcgccttttt cgcgggcttc
     8821 tttaccgcct tgtcagacct ggaagcgccc ttctctttct ccggcggcgc cagcatcgtg
     8881 ccgcgacact tacgcgcgcc gcaacggcat tcgtattcct tcttgagctt cttggtctgc
     8941 cgcgcgtcga tgacgaggcc gtagtcgtaa aagacttcct cgccctcggc gatgtcgcgc
     9001 aacgcgtgca catacacgtg cccgtcgatc tcttcggctt cgcagttcgg cgcacatgaa
     9061 tggttgatcc agcgcgcgct gttgccgtcc actttgccgt caatgacctt gccgctgtcg
     9121 agcgcaaagt agaacgtgtg attcggttcg tccggattat gcggatgacg gcgcaacgct
     9181 tccttccagg agatccgctc acccttgtat tcgatcagcc gctcgccggc cgcaatcggc
     9241 tcgatggcaa acacgccttt gccgtgcaca cccgaacggc gcacggcgat cctgcgtgaa
     9301 ctcattgaat gaatccttgt gaagaactgg gggtgaagat ccggcagccg ccagaaatgc
     9361 ccgcgtctaa cgcactgctt tttccgacgc ggatagcaaa cgcggcgcct tgcgagcgcc
     9421 gcgtcgatat gcgacgctcc atgcgtcaca tccggcatcg tacacgctca agacggcttc
     9481 gttcaaccgt caaaaagacg tgcgcgtgca aggcgcattg gccgattcac cgttgtccga
     9541 acgaaatgtc gccgaacagt gccttttgct cacgcggttg cgaacgccag tactgcggtg
     9601 gcgcttcgac cgtcgcgccg agctgcgctg cggcatgcca cggccaacgc gggttgtaca
     9661 gcaacgcgcg cgccatcgcg atcagatcgg catcaccctc ttcgatgatt tgttcagcat
     9721 gcagcggatc ggtgatcagg cccacgccaa tggtggtgag gccggttgcc tgtttgacgg
     9781 ccctcgcgaa cggaatctgg tagcccggct cgagcggaat cttctgcagc ggcgagacac
     9841 cgccggacga cacgtcgatc cagtcgcagc cgcgcttttt caactcctgt gcgaacgcga
     9901 ttgtgtcctc gagcgtccag ccgccttcga cccaatcggt cgccgagaca cgcacgccaa
     9961 ccggtttgtc ggcgggaaac gcggcgcgca caatgtcgaa gatttcgagc ggaaaacgca
    10021 tccggttttc gagggagccg ccgtagtcgt cactgcgttg attggcgatc ggcgacaaaa
    10081 actgatgcaa cagatagccg tgtgccgcgt gcacttccag cgcatcgatg ccgaggcgtg
    10141 cggcgcggcg cgcggacgct gcgaacgctt cgcgaatccg gttcagtgcg ggcgtatcga
    10201 gtgcgagcgg cggctcttca cccgccttgt gcggcaacgc cgacggcgcg tgcggcagcc
    10261 agccgccttg cgacaccgga atcagttgac cgccgtccca cggcgcatga ctcgatgcct
    10321 tgcgcccagc atgccccaac tgcatcgcga cgcgaatgtg cgaatgcttg cgaatcgcgg
    10381 ccagtaccgg ctccagtgcg gcttcggtgg catcgtccca cagtccgaga tcgcccggag
    10441 taatgcggcc atccggttcg acggcggtcg cctcgatgca taacatgccg gcgccggata
    10501 acgcgagact gccgagatgg atcatgtgcc acgccgtagc ctcgccacgt tcggcggaat
    10561 actggcacat cggggagacg acgatacggt tgggaagcgt cacgctacgc agcgtgagcg
    10621 gggaaaaaag cgcgctcatg aattacgccc agtaaaagcc cgagagccaa cgagcatagc
    10681 accgcgattg attatctgca gccgcgcaaa tcagagtttc ggctcgacct gatcgagcca
    10741 ctcgctgaac atgcggcgtg cagcagtttc cagcgccgga ccgaactgcg cggtctcggc
    10801 acgcaactga cgggcgtcga tcccgtggcc ggcaatctca cccgcgttgc cgatcagcca
    10861 gggttcgaac cggtcagcgc gaatctcagg atgacattgc agacccagca catggccgcc
    10921 ccaggcgaac gcctgatttt cgcaggcagg cgtcgaggcg agacgcgtgg cgttgtcggg
    10981 caaatcgaag gtatcgccgt gccagtgcag cattgacgta tgtgccccgt ccagatgacg
    11041 cagcggtgaa gcccggcccg catcggtgag cgtaagcggt gtccagccga gctcggtatg
    11101 gccggcggga tagacccggg caccgagtac acgcgcgatc aattgcgcgc cgaggcagat
    11161 gccgagcgtc ggcaaaccag cggcaatgcg cttttcgatc atggcgagca agggaacgag
    11221 cgttgggtaa agcgcgtcgt cggttgcact gatcgggccg cccagtacga ccatgagcga
    11281 tgcagcaact gggtccggtg cttcgatacg gccaacgccg acatcgagat aacgcaccgg
    11341 ccggccgcgt tcgccgagct ccagttcaag actgcccaga tcctcgaagt gcacgtggcg
    11401 aatggccaga acttcgcgat tcatagcccg tcccaccttc agtttgacta aagacttgcc
    11461 gctacgcggc gcgaaccgtc cggcaggcgg cgattgccgt ctgccggaac acggtttggc
    11521 agtgtagcgg attgtcccgg aagaagccgc cttattgggc gaatatcttc caggtcttct
    11581 tttgggtagc gacgtcggcc gtttcgtaca ctgagcaatc gagacgcaga tccgtgtccg
    11641 acatgttcat gtcgagcagt gcgcagtgat gcggcgtctt cttcggattc ttgctcggct
    11701 cgaagtgcgt gcagctcagg cacatgcgat gcggcgggat cgtgccttgc gcttccagtt
    11761 ggtggatcgt cttgagcagc gtgcgataga acagcgtctg ctcttcgtcg cgcagcgtgc
    11821 cgaccgcttt ggccaggaag tccggccatt gcgcagcgcg tttcgcggcg gtgcggccac
    11881 gcgccgtcaa acggaccgcg agggcgcggc catcgtcgag ggcacggcgc ttttcgacca
    11941 gacctttggt ctcgagcgtg ctcacggcat cgctggtggt ggcggccgtc aaggccgttt
    12001 cacgggcgat ttcgccaaga cgcatggggc ctttgcgctg catcagcaac accaggattt
    12061 cgccttgggt cggcgtaagg cctgcgccct ccgcccattc ccaagcctgg ctccgcatgg
    12121 ccgtgctcaa tcgcaatagg ctgtgggtca ctcgcccggc tgcctgttct ccgtatacgc
    12181 cttcgctcat aatcttcgtt tggtttattt aaagcccgcg tgcgatgcca gcgaagcgct
    12241 gccgagcaca cccgtagcct gtgtggggta gcctcgccca aaaacaggcg agacggtttc
    12301 tactatctca aaaatttacc gccttgcaag ccaaagacgg cgaaaacgac attctatgcg
    12361 agaccggcca atcgtacagc taagttatcc aacgcaagct gtggataacc atgggaaaac
    12421 gcagggatgg cctgtgcgtc cattcttgat aagcgatttg ttgcctgtgc cccctccaaa
    12481 gttgtcctcg tttaccggga ccgttacgcc tcggatcaac ccagttatgg tttacgtagc
    12541 gctttgactt ttcagggaat tattcagttg tccacaggca gatgcaatgc ttgttaacta
    12601 ctactacttg tgtatacgta aattttagaa aaacctgaag ccccccgatc tggaaattta
    12661 attacgaaaa aaaacccgca tgaagcgggt cttccttacg gtttgctgcg agccatcctg
    12721 tctctggcta ggctcgccac tgcagacact gctgccttac agcttcgttc aaggactttt
    12781 cctgttcgaa aacgccgcgc aaccacgtcg cgtcattgac ctgaccgcgc gcgatagccc
    12841 cgatttcggc gagcgcatta cccgaaccca atgcttcggc atgcggtgca atcaaatcca
    12901 gcgtttcgag gatgtcttcg gaaatcgtct tgcgttcgct tgtttgcgga tcgatacagt
    12961 tgccggccag accgaagcgg catgcctcga aacggttgaa cgtgtagacg aggtagtcgt
    13021 cttccttcgg cgtgatcggc ttgtccagca acagatgccg cgcaagcgtc tggatgtagc
    13081 aggcaatcgc cgcggcgcga tccactgaaa acggcgtatc catcacgcgc acttcgatcg
    13141 tgccgaaccc gggcttcggc cggatgtccc agtaaaaatc cttcatgctg ttgaccacgc
    13201 ccgtgtggac catcttcgag aagtattcct cgaagctatc ccacgtcaac acgaacggcg
    13261 cacggcccga cagcgggaat gcgaacacgg aattcaggcg tgccgaatga aaaccggtgt
    13321 cgacgccttg cacgaacggc gacgaagcag acagcgcgat gaaatgcgga atgaagcgcg
    13381 acatcgaatg gagcagatac agcgcgctgt tcgggttcgg gcagccaatg tgcacatgct
    13441 ggccgaacac cgtaaattgc ttggcaaggt agccgtacaa ctccgaaata tactgaaagc
    13501 gcggcgtatc gacgatctgc cgttcgctcc attgctggaa cgcatgcgtg ccgccgccgc
    13561 acaagccgac gttcaggcga tcggccgcgg aaaccagcgt atcgcgaatc ttgcgcaaat
    13621 cggtgacggc ctgctcgtgc gtcgtgcaga taccggtcga cagctcaatc atgctttcgg
    13681 tgatttccgg cgtgatgttg ccggggattt tttcgtcctt gatgagacgc atcaaatccg
    13741 agccggcttt ggtcaaatcg tagtcgtgcg tattgacgat ctgcatctca agttcgacac
    13801 cgaaagtaaa cggtttcgaa tcgatgaagg gttcgagtga catggcgtcc cttgagtcag
    13861 gtcggccggt gtgcggcaca tgcttgtgtc acatgcccgg ccgtttttga atcggataag
    13921 ttggataaaa cacgcaaatc aggcgcctga agcatcgagc agcggcgctg gcttactctt
    13981 cgcgccgttc ggccaccatt gcgaggctgc gatacacgag ccacggcccg agaatttgca
    14041 gcacgaaaat cgaacacatc acgatggccc gcaattgcgg atcgaaattc ggataaaggt
    14101 tgtacgtatc gtcgacgagc agatatgcga gcgccgacat gggc
//
LOCUS       DATASH010000592         1863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4053091,
            whole genome shotgun sequence.
ACCESSION   DATASH010000592 DATASH010000000
VERSION     DATASH010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4053091"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..509)
                     /locus_tag="VHV99_16060"
     CDS             complement(138..509)
                     /locus_tag="VHV99_16060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012429005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636971.1"
                     /translation="MANTEYTLSHQDVVKALIIDQDIHEGFWTLNVNFNVAASHGPRV
                     PDPSLTVTIKGIGIMRAPPTDPFAIDAALINPTPPQPVEGTVHITSAVNGTHGNSMQF
                     CRDVYRNVAVQSPIKAPIKRG"
     gene            complement(971..1771)
                     /locus_tag="VHV99_16065"
     CDS             complement(971..1771)
                     /locus_tag="VHV99_16065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3636972.1"
                     /translation="MEKLLANQVALITGANSGIGHGVARSLAQAGAAVVLNYHSHSEG
                     AEQLASDIEQAGGAAFAHRADVSDPAQVDAMFAACRARFGSVDIVVANAGLQKDSAFA
                     EMTLHDWQTVLSTNLTGQFLTSQAAVKEFRRRGPSPGSKALGKIICMSSVHEVIPWAG
                     HVNYAASKGGIQMFMKSLAQEVAPERIRVNSIAPGAIRTPINESAWDTPDALAKLLLL
                     IPYGRVGDVEDIGKAAVWLASDESDYVVGTTLFVDGGMTLYPCFADNG"
BASE COUNT          358 a          547 c          580 g          378 t
ORIGIN      
        1 aatcggttga tgggcggctt gctggtcggc atgggtgcgc tgatggcggc ggtacgctga
       61 ttcgagcaat cgcataccgc cggccgtgcc gcgttccgtt gcgccgctaa tcgctgaacg
      121 cggaggcttc ggcccgatca gcctcgttta atgggcgctt taatcgggct ctgtactgcg
      181 acattgcggt acacgtcgcg acaaaattgc atgctgttgc catgcgtgcc gttgacggcc
      241 gacgtgatgt gcacggttcc ttcgaccggt tgaggcggag ttggattgat gagtgcggcg
      301 tcgatcgcaa atggatcggt tggcggcgcg cgcatgatgc cgatgccctt gatcgtcaca
      361 gtgaggctgg gatcgggcac acgcggcccg tggctcgcgg cgacgttgaa attgacgttg
      421 agcgtccaga aaccctcgtg aatatcctga tcaatgatga gggccttcac cacgtcctgg
      481 tgcgacagtg tgtactcggt gtttgccata atccctcctt cctcgtgcgc gcggcgcacg
      541 tgtgcagaaa tggcattttg cggactggtg aactagtttt acgcgctatt ttgcgcagtg
      601 caagcagaaa cgctcgtctg ccaacgggta gcgccaagcc tgcgtgaacc ggtgcagaca
      661 tgcgaaaggc gacgccgcaa acgtgggcat cgcctttcat ccagccgttg atcgaactac
      721 atcgcaatag agggaccgtc gcatcgacgg catgcgcctg cggcgcgtgg gttagtgaaa
      781 gatcagataa aggatgacga gtacgatcaa cggtacgcca agcaaccagc cgagtaggta
      841 aggcatgatg tgacctcctt tcgtatcgaa gtttgaatac gatggtgatt cagcatgcac
      901 cgtgcccgac ctgactcaag tgacgccgca agatgtgggt gaggcaggca gcgggccgca
      961 cgacgcatcg ctaaccgtta tcggcaaagc acggatacaa ggtcatcccg ccgtcgacga
     1021 acaaggtcgt gccgaccacg tagtcgcttt catccgaagc gagccaaacg gcggccttgc
     1081 cgatgtcctc cacgtcgccc acgcggccat atggaatcaa cagcaatagt ttggcgagcg
     1141 cgtcgggcgt gtcccacgcg ctctcattga tcggcgtgcg gatcgcaccg ggcgctatgg
     1201 agttcacacg aatgcgctcg ggcgcgactt cctgcgcgag cgatttcatg aacatttgga
     1261 tgccgccctt cgaagccgcg taattgacgt gtccagccca cggaatcact tcgtgaacgg
     1321 agctcataca aatgatcttg cccaacgctt tggacccggg gctgggtccg cgtcggcgga
     1381 attccttgac ggctgcctgg ctcgtcagga actggccggt cagattggtc gacagcacgg
     1441 tttgccaatc gtgcagcgtc atttccgcga acgcgctgtc tttctgcaag cccgcattcg
     1501 ccaccacgat atcgacactg ccgaaacgcg cacggcacgc agcgaacatc gcgtcgacct
     1561 gtgcgggatc ggacacgtcc gctcgatgcg cgaacgctgc gccgcccgct tgctcgatat
     1621 cgctggcgag ctgctcggct ccttcggaat gcgaatgata attcagcacc acggccgcgc
     1681 ccgcttgcgc cagcgatctt gcgacgccat ggccaatgcc cgaattggcg ccagtgatca
     1741 acgcgacctg gttggcgagt aacttttcca ttggatgctc cggcgtgaca tggtgcgtcg
     1801 ggggtcctaa tgcgacggcg ccgccggctc ccatgcggcg cattgcatgg gagccggcgg
     1861 cag
//
LOCUS       DATASH010000593         6473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6031601,
            whole genome shotgun sequence.
ACCESSION   DATASH010000593 DATASH010000000
VERSION     DATASH010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6473
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6031601"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..935)
                     /locus_tag="VHV99_16070"
     CDS             complement(51..935)
                     /locus_tag="VHV99_16070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006401481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX3636973.1"
                     /translation="MEIFGVPLPAMLSQLLLGLVNGSFYAILSLGLAVIFGLLNVINF
                     AHGALFMLGAMLAWMGFSYFGLPYWVMLVLAPLIVGVFGIVIERSMLRWLYKLDHLYG
                     LLLTFGLTLVVEGVFRSIYGSSGQPYDVPSALSGATDLGFMFLPNYRAWVVVASLVVC
                     FVTWFVIEKTRLGAYLRAGTENPKLVEAFGINVPLMITLTYGFGVALAAFAGVLAAPV
                     IQVSPLMGQPMIITVFAVVVIGGMGSIMGSILTGLMLGVIEGLTRVFYPEASATVVFV
                     IMALVLLVRPAGLFGKEK"
     gene            complement(1131..2345)
                     /locus_tag="VHV99_16075"
     CDS             complement(1131..2345)
                     /locus_tag="VHV99_16075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3636974.1"
                     /translation="MKKNPLARLATLCFAVAAGTALTMSSAQAADDAVKIGFITDLSG
                     LYADIDGQGGLEAIRMAIADFGGKVNGKPITLVYADHQNKADIAASRAREWFDRDGVD
                     MLIGGTNSATGLSMNTVAGEKHKVYINVGAGADTLTNEQCTPYSIHYAYDTTALANGT
                     GSAVTKQGGKTWYFLTADYAFGKALEKATSDVVKANGGQVLGTVRHPLSASDFSSFLL
                     QAQASKAQILGLANAGGDTVNSIKAAKEFGITKTMKLAALLMFIDDVHSLGLETTQGL
                     VLTDSWYWNKDAATRKWAQRYFDKMKKMPSSLQAADYSATMTYLNAVKAVGSTDSDKV
                     MAQLKKTKIDDFYAKGYIRQDGSMIHDMYLMQVKTPAESKEPWDYYKITATIPGEQAF
                     TTKAQTRCALWK"
     gene            complement(2412..3170)
                     /locus_tag="VHV99_16080"
     CDS             complement(2412..3170)
                     /locus_tag="VHV99_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3636975.1"
                     /translation="MNTIAERESLEVSGTAGGAPALEIAGLQAWYGESHILHGVDLTV
                     NRGEVVTLLGRNGAGRTTTLRAIMGLTGRRTGSIRIDGRETINLPTHRVAHCGIGYCP
                     EERGIFSSLSCEENLLLPPPVGDKAHMMSLEEIYSMFPNLQERRMSQGTRLSGGEQQM
                     LAVARILRTGASLLLLDEISEGLAPVIVQALARMIMTLKARGYTVVMVEQNFRFAAPL
                     ADRFYVMEHGAIVEHFGAGELESKMPVLHDLLGV"
     gene            complement(3167..3253)
                     /locus_tag="VHV99_16085"
                     /pseudo
     CDS             complement(3167..3253)
                     /locus_tag="VHV99_16085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917939.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(3256..4035)
                     /locus_tag="VHV99_16090"
     CDS             complement(3256..4035)
                     /locus_tag="VHV99_16090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3636976.1"
                     /translation="MILGDTILETRGLTREFKGFIAVNGVNLRVRRGSIHALIGPNGA
                     GKTTCFNLLTKFLEPSAGQIVFNGVDITNERPAQIARRGIIRSFQISAVFPHLTALQN
                     VRIGLQRVLGTAFHFWKSERTLRQLDDRAMDLLTQVGLTDFADVLTVELSYGRKRALE
                     IATTLAMEPELMLLDEPTQGMGHEDVDRVTALIKKVSSGRTILMVEHNMNVIAGISDT
                     ITVLQRGEVLAEGSYAEVSKNPLVMQAYMGSADAALAGAHA"
     gene            complement(4489..6198)
                     /locus_tag="VHV99_16095"
     CDS             complement(4489..6198)
                     /locus_tag="VHV99_16095"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015004096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HEX3636977.1"
                     /translation="MSNTKPHAARRTEGEFDYIIVGAGTAGCVLANRLTEDPEIQVLL
                     LEAGGKDDYHWIHVPVGYLYCIGNPRTDWLYKTQSEPGLNGRSLSYPRGRVLGGSSSI
                     NGMIYMRGQREDYDDWARVTNDDSWSWNAVLPIFKRSEDHHAGASESHGAGGPWRVEK
                     QRLKWKILEEFSQAAQQTGIPATEDFNRGDNTGVGYFDVNQKRGIRWNASKAFLRPAL
                     KRPNLTVITGAHTQRVVFEGRRCTGVEYRGDNTDYLAKARCEVILSSGAVNSPQLLEL
                     SGIGNGARLQNLGIEVVNDLHGVGENLQDHLQLRMAYKVDGVRTLNTASAHWWGKLMI
                     GMQYALFQSGPMSMSPSQLGAFAKSDPDDRSLTRPDLEYHVQPLSLDRFGEPLHRFNA
                     FTASVCQLRPTSRGSIHIESADPSAPPLIAPNYLSTDYDRHVAANALRLTRRIAAAPA
                     LARYRPQEILPGIQYQTEEELRQAAGAVGTTIFHPVGTCRMGATDDPGAVVDNRLRVI
                     GVDGLRVVDASVMPSITSGNTNSPTLMIAERASDMIREDRRARVASSEVVQTSAAPSM
                     SAV"
     gene            complement(6267..>6473)
                     /locus_tag="VHV99_16100"
     CDS             complement(6267..>6473)
                     /locus_tag="VHV99_16100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636978.1"
                     /translation="AGGVSRFLCLSFFAAAKKVSAAPHRGNANRPITIQGKAKESDQG
                     KANTVGNQTNKRRTGNHMKERAKS"
BASE COUNT         1187 a         1967 c         1976 g         1343 t
ORIGIN      
        1 caccgcgaga agcgcgatca gcagcagacc gtagagcact tttctctgca tcatttttcc
       61 ttgccgaaga gacccgccgg acgcaccagc aacacgagcg ccatgatcac gaagacgacg
      121 gtcgccgacg cttccgggta aaacacacgc gtcagccctt cgatcacgcc taacatcagg
      181 ccagtcagaa tcgagcccat gatcgagccc atgccgccga tcaccactac cgcgaacacg
      241 gtgatgatca tcggctggcc catcagtggc gagacctgaa tcacaggcgc ggcaagcacg
      301 ccggcaaacg ccgccagcgc gacgccgaag ccataggtga gcgtgatcat cagcggcacg
      361 ttgatgccga acgcctcgac cagtttcggg ttttcggtgc ccgcgcgcag ataggcgcca
      421 agacgcgtct tctcgatcac gaaccacgtc acgaagcaga ccacgagcga cgccaccact
      481 acccacgcgc gatagttcgg caggaacata aagccgagat cggtcgcgcc ggagagtgcc
      541 gacggcacgt catacggctg gccggacgaa ccgtagatcg aacggaacac gccttcgaca
      601 accagcgtga gcccgaaggt cagcagcagc ccatacagat gatcgagctt gtacagccag
      661 cgcagcatgg aacgttcgat cacgatgccg aacacgccga cgatcagcgg cgcgagcacc
      721 agcatcaccc aatacggcag cccgaaatac gagaacccca tccacgcgag catcgcgccg
      781 agcatgaaca gcgcgccgtg cgcgaagttg atcacgttga gcaagccgaa aatcacggca
      841 agacccaggc tcagaatcgc gtagaacgag ccgttcacga gccccagcag caactggctc
      901 agcattgccg gtagcggaac gccaaagatt tccattgaag ccttaatccc ttaagccatt
      961 gccagggtga gatcggtcat gcacgatccg gccggcgtgc gagcggtgaa ccatgcgccg
     1021 atcgcacgcg gcatgtgaaa cgccttaccg tcggtctgtg taccatcgcg gcgccatcga
     1081 tctcccgtca gacggagctc aacggcggcc gtgtggttat caacccgcgc ttatttccac
     1141 aacgcgcagc gcgtctgggc cttggtcgta aatgcctgtt cgcccgggat cgtcgccgtg
     1201 atcttgtagt agtcccacgg ctccttggat tcggccggcg tcttcacctg catcaggtac
     1261 atgtcgtgga tcatgctgcc gtcctgacgg atgtagccct tcgcgtagaa gtcgtcgatc
     1321 ttggtcttct tcagttgcgc cattaccttg tccgaatcgg tcgagccaac tgctttcacc
     1381 gcgttcagat aggtcatggt cgccgagtaa tcggccgctt gcaggctcga cggcatcttc
     1441 ttcatcttgt cgaagtagcg ctgcgcccac ttccgcgtgg cggcgtcctt gttccagtac
     1501 cagctgtccg tcagcaccaa gccttgcgtg gtttccagcc ccaggctgtg cacatcgtcg
     1561 atgaacatca gcagcgcggc cagtttcatt gtcttggtga tgccgaattc cttggccgcc
     1621 ttgatcgagt tgaccgtgtc gccgcccgcg ttggcaaggc cgagaatctg cgctttcgac
     1681 gcctgcgcct gcagcaggaa cgacgagaag tccgatgccg acagcggatg ccgcaccgtg
     1741 ccgagcacct gcccgccatt cgccttcacc acgtctgagg tggctttttc cagcgccttg
     1801 ccgaaagcgt agtcggccgt caggaagtac cacgtcttgc cgccctgctt cgtcaccgcc
     1861 gaacccgtgc cattggcgag cgccgtcgtg tcgtacgcat agtggatcga gtatggtgtg
     1921 cattgctcgt tggtcagcgt atcggcaccg gcgccgacat tgatgtagac cttgtgtttc
     1981 tcgccggcca ccgtgttcat cgacagaccg gtcgccgagt tcgtgccacc gatcagcata
     2041 tcgacgccgt cacggtcgaa ccactcacgc gcgcgcgaag cggcgatgtc ggccttgttc
     2101 tggtgatccg cgtagaccag cgtaatcggc ttgccattga ccttgccgcc gaagtcggcg
     2161 atcgccatgc ggatcgcttc gaggccgccc tggccgtcga tgtccgcgta cagccccgac
     2221 agatcggtga tgaagccgat cttgaccgca tcgtcggccg cttgcgcgct gctcatcgtc
     2281 aacgcggtgc cggcggcaac cgcgaaacaa agggtggcaa gccgcgcgag tgggttcttt
     2341 ttcattcctg tctcctggtt tcttcgcttg tggttatcag aggcaatcgg acgcttctcg
     2401 ttacttcctg tctatacgcc cagtaggtcg tgcagtaccg gcatcttgct ttcgagctca
     2461 ccggccccga agtgctccac gatcgcaccg tgctccatca cgtagaagcg atcggcgagt
     2521 ggcgcggcaa agcggaaatt ctgttcgacc atcacgaccg tgtagccgcg cgccttgagc
     2581 gtcatgatca tgcgggcgag cgcttgcaca atgacgggcg cgaggccttc cgaaatttcg
     2641 tcgagcaaca gcaaactcgc tccggtccgc agaatgcgcg cgacggccag catctgctgc
     2701 tcgccgcccg agagccgcgt gccctggctc atgcggcgtt cctgcagatt cggaaacatc
     2761 gaatagattt cttcgagcga catcatgtgc gccttgtcgc cgacgggggg cggcaacagc
     2821 agattttcct cgcacgagag gctcgaaaaa atcccgcgct cttccgggca atagccgatg
     2881 ccgcaatggg cgacgcgatg cgtgggcaga ttgatggttt cgcgtccgtc gatgcggatc
     2941 gaaccggtgc gccggccggt cagacccatg atcgcgcgca atgtggtggt gcgccccgcg
     3001 ccgttgcggc caagcagcgt gacgacttcg ccacggttca ccgtcagatc gacgccatgc
     3061 aggatatggg actccccgta ccatgcctgc aaacccgcga tttccagcgc gggggcaccg
     3121 ccagccgtgc cgctcacttc gaggctttcg cgctcagcaa tcgtgttcat gcgtgggctc
     3181 cggcaagcgc cgcatccgcg ctgcccacat gggctcgcat cacgcgcggg ttcttcgaca
     3241 tttccgcaat cgtgttcatg cgtgggctcc ggcgagcgcc gcgtccgcgc tgcccatgta
     3301 ggcctgcatc acaagcgggt tcttcgacac ttcggcgtag ctgccttcag cgagcacttc
     3361 gccccgttgc agcaccgtga tggtgtcgga gataccggcg atcacgttca tattgtgctc
     3421 gaccatcagg atcgtgcggc cgctcgatac tttcttgatc aatgcagtga cacgatcgac
     3481 gtcttcatgg cccatgcctt gcgtcggctc gtcgagcaac atcaactcgg gctccatcgc
     3541 cagcgtggtg gcgatttcca gggcgcgttt gcggccatag gacagttcta ccgtgagcac
     3601 gtcggcgaaa tcggtcagtc cgacctgggt gagcaaatcc atggcgcgat cgtcgagttg
     3661 gcgcaacgtg cgttcgctct tccagaagtg aaatgcggtg ccaagtacgc gctgcaaacc
     3721 aatgcgcacg ttctgtaatg ctgtcagatg cggaaatacg gcagatatct gaaatgaccg
     3781 gatgatgccg cgccgggcga tttgcgccgg gcgttcatta gtgatgtcga cgccgttgaa
     3841 gacgatctga cccgcgctcg gttcgagaaa tttggtgagc agattgaagc aggtggtctt
     3901 gcctgccccg ttagggccga tcagcgcatg aatcgagcca cggcgcacac gcaggttcac
     3961 cccgttcacg gcgatgaagc ctttgaactc gcgggtgagg ccgcgcgttt cgagaatcgt
     4021 atcgccgaga atcatgttcc cttccatacg gagtaaggcg aagcactacg atcttccgcg
     4081 cgaaaccctg cgcgacgttt gatgacggcg acacccccgt gtcactcgtt ggcgtgctgc
     4141 accaatccgg acggtgatcc atggggcggc acgcagcatg gctgccattg tcgcgccaat
     4201 gatgcagcgc attcattggg atttgcactt agtggatgac gactgcgtga tcgaggcctg
     4261 cgctggcccg atgcgtgcgg gagtggtgca tcgatcttgc tccggacagg catgggggat
     4321 gcccgagcag tccctgttcg cggcgtgtcg agggttgccc gaaccacgct catcttgctc
     4381 ggggctgtgt atggggcatg cccgagcagt ggttcaaggt tgctcgatgc atgccaggga
     4441 aagtccgagg cgcgcgccgc ggcctcggct tgcctgccac attgcgtgtc acacggcgga
     4501 cattgacggc gcagcgctcg tctgaaccac ctcgctgctg gcaacgcgtg cgcggcggtc
     4561 ttcgcggatc atgtcgctgg cgcgttcggc gatcatcagg gtcggcgaat tggtgttgcc
     4621 cgaggtaatg gacggcatga ccgacgcatc gaccacacgc aagccgtcaa ctccgataac
     4681 tcgtaggcgg ttatcgacta ccgcgccggg gtcgtcggtg gcgcccatgc ggcaggtgcc
     4741 gaccggatga aaaatggtgg tgccgaccgc gcccgcggcc tgccgaagtt cttcttcggt
     4801 ctggtactga ataccgggca gaatctcttg cggccgatag cgcgcgagcg caggcgccgc
     4861 ggcaatgcga cgcgtgagac gcaatgcgtt ggcagcgacg tgccggtcgt aatcagtgga
     4921 cagatagttg ggcgcgatca gcggtggcgc cgaaggatcg gccgattcga tgtgaatgct
     4981 gccgcgcgag gtcggccgca actggcacac cgacgccgtg aacgcattga agcgatgcag
     5041 cggttcaccg aagcgatcga gcgagagcgg ctgcacgtga tactcgaggt cggggcgcgt
     5101 gagcgagcga tcatccggat cggatttggc gaacgcgccg agttgcgatg gtgacatcga
     5161 cattggaccg ctttgaaaga gcgcgtactg catgccgatc atcagcttgc cccaccaatg
     5221 agcggaggcg gtattgagcg tgcgcacgcc gtcgacctta taggccatgc gcaattgcaa
     5281 atggtcctgc agattttcgc cgacgccgtg cagatcattc acgacctcga tgccgagatt
     5341 ttgtagccgt gcgccatttc cgatacccga tagctcgagc aattgcggcg agttgaccgc
     5401 acccgaactg aggattactt cacaacgggc tttggcaaga tagtcggtgt tgtcgccgcg
     5461 atattcgaca ccggtgcagc gacgtccttc gaacacgacg cgctgtgtgt gtgcgccggt
     5521 gatgacggtg agattcgggc gcttgagcgc cggacgcaag aatgctttcg acgcgttcca
     5581 ccggataccg cgtttctgat tgacgtcgaa gtagcccacg ccggtgttgt cgccgcgatt
     5641 gaagtcttcc gtggcgggta tgccggtttg ttgtgcggct tgtgaaaact cttcgaggat
     5701 cttccatttc aggcgttgct tttcgacgcg ccatgggccg cccgcgccat gcgattcaga
     5761 cgcgccggcg tggtggtctt cgctgcgttt gaagatgggg aggacggcgt tccaggacca
     5821 ggagtcgtcg ttggtgacgc gggcccaatc atcgtagtcc tcgcgctggc cgcgcatgta
     5881 gatcatgccg ttgattgatg aactgccgcc tagtactctg ccgcgcgggt atgagaggga
     5941 gcggccgttc aggccgggtt ctgattgggt tttgtagagc cagtcggtgc ggggattgcc
     6001 aatgcagtag agatagccta ccggcacgtg gatccagtgg tagtcgtctt tgccgccggc
     6061 ttccagcagg agaacctgga tttccggatc ttcggtcagg cggttggcca gcacgcagcc
     6121 tgccgtgccg gcgcctacta tgatgtagtc gaattcgcct tctgtacgcc ttgcggcgtg
     6181 gggttttgtg ttgctcattg ttttgtctcc taagcttgtt gcctgcggcg gtggcctttt
     6241 tttgattcgt ttgcctgctt ctttgcctac gactttgcgc gttccttcat gtgattgccc
     6301 gtgcggcgct tgtttgtctg gttgcctacg gtgttggcct ttccttgatc tgactctttg
     6361 gcctttcctt gaattgttat tggtctatta gcgttgcccc tgtgcggggc ggcacttact
     6421 ttctttgccg ccgcaaagaa agataagcaa agaaagcggc tcaccccgcc agc
//
LOCUS       DATASH010000594        21776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1088743,
            whole genome shotgun sequence.
ACCESSION   DATASH010000594 DATASH010000000
VERSION     DATASH010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 21776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21776
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1088743"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VHV99_16105"
     CDS             complement(<1..236)
                     /locus_tag="VHV99_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010090096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX3636979.1"
                     /translation="MKKLLAALTVALLATVSIGAHAKDWSTIRFGVDASYPPFESKGS
                     DGKLVGFDIDLGNEICARLKAKCVWVENDFDGMIP"
     gene            complement(483..1157)
                     /locus_tag="VHV99_16110"
     CDS             complement(483..1157)
                     /locus_tag="VHV99_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX3636980.1"
                     /translation="MSSEREASGASGREPILHKQLSELVIARLRESITSGAYAPGDRL
                     VEGRIAEELNVSRVPVREALRALASEGLVEVRPRHGAIVASLDPSAAREMVQIRATLE
                     GLNARLAAEHCTPELVRQIERVLQEGNVKVAAGETAGLLELNTRFHDLLYSAGANSML
                     SDLMRSLRDRTRMLFVNANDAEVRTTWEEHAAILRAVQSGDATLAALLAERHVMRAAQ
                     HYLERF"
     gene            complement(1353..1631)
                     /locus_tag="VHV99_16115"
     CDS             complement(1353..1631)
                     /locus_tag="VHV99_16115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006216990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX3636981.1"
                     /translation="MIQITFLTNGGKTVQAPANSNLLRVSLREQGGIPFKCGGGLCGT
                     CKCRLEEGRENTDTVKQKELRHLSAQELEAGYRMACQTFVNGDVSVSW"
     gene            complement(1642..1971)
                     /locus_tag="VHV99_16120"
     CDS             complement(1642..1971)
                     /locus_tag="VHV99_16120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636982.1"
                     /translation="MYIILTSKPGQYRSEATDGITPVGAYDYFYCDRHIATFVLASIG
                     KDSRVRIVDESSPPTVNLVPTRFFEKFSTREAALISIQELAGVTQADAQLVERNTSAE
                     HGKTAFD"
     gene            complement(1995..2303)
                     /locus_tag="VHV99_16125"
     CDS             complement(1995..2303)
                     /locus_tag="VHV99_16125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX3636983.1"
                     /translation="MPTVVFHKNGEVYRDTVKENTNLVVRAGIRQFPFPHLRYECGMG
                     KCSRCACRVIAGAEQLPPPNWKEKKQLGERLNAGYRLACQLWIEHDVELAQDEAGDAQ
                     "
     gene            2547..3473
                     /locus_tag="VHV99_16130"
     CDS             2547..3473
                     /locus_tag="VHV99_16130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX3636984.1"
                     /translation="MIHLNDQQIDELIDLEQAIPALDDAFRQLGRGEAAVQRRVRTEA
                     NGVKLSTLGAVLPGAEVVGAKVYTTIAGKFNFSIVLFSTSDGSLLATLDANALTRLRT
                     AATTAIAVRALARPAATSAAIFGTGVQGTAHAHALARFTNLQSLHIVGLEGSDALAAE
                     LNARYGARGFSAQASQAQAAVEQADVIVTATRATGPLFDGHAVKPGALIAAIGSSLPT
                     TRELDDATIARASRIVVELREQAREEAGDLVQAAPGIVDWTAVVELGDVLEGKAPGRA
                     RDEDVIVYKAVGIGLQDVALAGLAYRTYLASR"
     gene            complement(3555..4313)
                     /locus_tag="VHV99_16135"
     CDS             complement(3555..4313)
                     /locus_tag="VHV99_16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX3636985.1"
                     /translation="MSVHTGMELNFVSPQLVHEGTAILQIVFINVLLGGDNAIAIAMA
                     CRSLPARQRRLGVWIGTSLGIVLRLAMIYLIGFLLAIPYMHVIAALLLVWIGIQLLRT
                     DDGGGETSAGRSTALWRAIVVILFADLSMSLDNGLATAAIAEALPESSRILVVLAGLA
                     AGAPVIALGSTLMLKLLGKFPLLTYAGAALLGWIAGEMLLADSQVSGALQQTWAAVAG
                     DTAGGNALRIALCVLVAAVVPLAGRLGMRRQCES"
     gene            complement(4310..5875)
                     /locus_tag="VHV99_16140"
     CDS             complement(4310..5875)
                     /locus_tag="VHV99_16140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636986.1"
                     /translation="MHPVASLTLAHYCYLVGVVAIIATMIFRANVVVPAILATFAVAF
                     AFSGKLLVGLQAIFMGSLTAAHELFNIFLVIALMTALLNGLRELGADVRMVKPFKRIM
                     VNGHVAFFILALITYLISLFFWPTPAVPLVGAILIPAAVYAGLPPLGAAAAIAIAGQG
                     MALSSDYVIKVAPGISAKAAGADMLSVANMGFTLSIVVGAVALGLTYLFLVRKNVKAP
                     GTANLIAWETGVAAVVSPAEEHQGSFAKVELARGTSGAPGELFHAPLAASAAAALPLS
                     GIAPAAPLDRREMWSKVFSVVTPLAFLAVVVVMTSPHMIGAEVIRGGDAAALIGGMAA
                     VLMMLATLSHGRKGFLDRTAEHIIDGLVFAFKAMGAVLPIAGFFFVGDSSTAGSILGI
                     APGAHVPSFLFELVQACSQLIPKSPLFVSFGVLLMGMITGIDGSGFAGLPLTGSLSGA
                     LAPMVHMPAATLAAIGQMGAVWTGGGTLVAWSSLIAVTGFARVPVLEAVRVLFLPVVC
                     GLVVATLVAVSIS"
     gene            complement(6124..7158)
                     /locus_tag="VHV99_16145"
     CDS             complement(6124..7158)
                     /locus_tag="VHV99_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018418281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX3636987.1"
                     /translation="MNYQVTWIEEGRSFEASSSETVLDAALRAGVPLPSECRFGGCGT
                     CRVKLVEGRVQYDEQPLGLAPEEEVAGYALACQARPSADLRISTDRPLASPVAPTRHC
                     ATVEALEPLAHDVVKLTLSVKGTAPFDFRPGQYMRVLLSEAGTRSFSMASSPGAPTAD
                     FHIRRIQGGYFTGQLLANLRVGDQLDVELPLGGFGYHEEDFRPLVMVATGTGIAPLKS
                     MLEQMLPDPGCPPVALFWGARAPADLYLHDALTELATRYPDFTYTPVLSRADDTWDGS
                     RGYVQQAVVAAYPDLSEHALYLCGSPDMIADAKRVFVEHGASVRYFYSDGFGFHHADA
                     GVAQASPAGL"
     gene            complement(7176..7655)
                     /locus_tag="VHV99_16150"
     CDS             complement(7176..7655)
                     /locus_tag="VHV99_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX3636988.1"
                     /translation="MKAFFKLELAEARHMIQAAIARSTEIGVLESVCVVDEGGFPLAL
                     ERMDGGRITGPQIAWNKAFTAAGHKRSTHLFTTPPNGPALPGNEAFGIQWSFEGKFAA
                     FVGGFPIVVDNQVVGGIGLSGGNGEQDTAAGLAGLAALRDLLRDANREVLVQSDIKL"
     gene            complement(7691..9145)
                     /locus_tag="VHV99_16155"
     CDS             complement(7691..9145)
                     /locus_tag="VHV99_16155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX3636989.1"
                     /translation="MTNVQTYLNYIDGEWCASLSGATFDNINPADTRDVVGRFQASSA
                     ADATAAVKAAAGAFAQWKKLSPSARSKILLNAATYLEQHAERFAEELTREEGKQKNLA
                     KDEFLRSAQTIRYYAIEAQSFGGETFPNDDPDMAVYTLREPLGVVTVISPWNFPVSIP
                     ARKIAPALVCGNTVVFKPSSDAPLSGLRLTEAFVEAGIPKGVLNFVTGKSSEVGPVIV
                     AAPDIRAISFTGSTSAGEQIHRAAAMSTRTQMELGGKNPLIVLDDADVDRAVDLTVKG
                     GFSLTGQACTGTSRVLVTPQVKHAFIAQLVEKARALKIGNGMTPGTEIGPLATEGQLR
                     TVLDYIEIGKREATLVTGGNRITSGDFGHGFFVEPTVFADVARTARIAREEIFGPVIA
                     IIEVADYADAISVANDTEYGLSAALVTRDPKLMHRFAEDIAAGTVKINRTTTGNLINA
                     PFGGLKNSSTATFRESGREGLAFYLQTKTVYRGV"
     gene            complement(9249..9980)
                     /locus_tag="VHV99_16160"
     CDS             complement(9249..9980)
                     /locus_tag="VHV99_16160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX3636990.1"
                     /translation="MAELMNRDAFRAALEQAIKGKSANQAPFSVAWASGKLSRTHLAR
                     WAENHYHYVGPFADYLGYLYARTPDHMTEAKDFLLANMYEEEIGGDRHTDLLIRFAEA
                     CGTTRERVVDPDNMSPTTRGLQGWCYSVAMREEPVVAVAALVVGLESQVPSIYRKQTP
                     TLREQYKFTDEEVEFFDLHIVSDEIHGERGYQIVLEHANTPALQQRCLKICEVGAQMR
                     LLYTTALYYDYVEKEIPLPELGMAA"
     gene            complement(10097..11191)
                     /locus_tag="VHV99_16165"
     CDS             complement(10097..11191)
                     /locus_tag="VHV99_16165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX3636991.1"
                     /translation="MKKSICTAAALASLSVTAHAQGSVTLYGLIDASVAYTTNQGGGK
                     TWAMGSGATRGSRVGFVGSEDLGGGTRAIFRLENGFDITDGSLGQGGLMFGRQAYVGI
                     ENDRYGRLTLGRQYESMVDYVAQLTAADWSVYMEHPGDMDLTNRGVRVNNSVRYGNSF
                     GGFTGSAMYSFGGQPGSFGKDSMWSLGGSYANGPVYLGFGMTYARQPGDLSPGTFWKS
                     TNSAVGTYALAAHSFLTVGGGGSYTIGSAKISADITSVSYNQGFDGQNVDFMNYEVNA
                     LYNFTPAFSAGLGLTYTDGQVDANNADPRYLQYNAMLTYSLSKRTQLYGFATFQRALR
                     DAQFAQVTQFIAASSNNEQGALGIGLRHSF"
     gene            complement(11368..11591)
                     /locus_tag="VHV99_16170"
                     /pseudo
     CDS             complement(11368..11591)
                     /locus_tag="VHV99_16170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008652157.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
     gene            11914..13053
                     /locus_tag="VHV99_16175"
     CDS             11914..13053
                     /locus_tag="VHV99_16175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX3636992.1"
                     /translation="MHVIFTADEKCGFKAPFRRRFAAAAARGSSLRRSGFLTLLISAV
                     FCCSLLSSGPVAAAEKILRVLAWPGYADADVVKTFEARYNAKVEVTLVDSDEALWAQM
                     HTQSTPPFDVLAANTAEIQRYTRANLLAPLDLASLPNTRRQMPRFQALSSIEGLTSAG
                     KVYAIPFTYSSMGLIYDRKQIAVAPRSMRELWNPRYRGKVLDFNSAQPNFSFTALALG
                     YRHPFQLDAAQMRVITQKLVDLRRNLLTYYTLPEEATAFFIQHKVALMFGNYGTQQVE
                     SLRRAGADVGYVIPDEGALAWLDCWSMTRSATDRPLALAWINYMLEPDVSALLTQRQG
                     LANTLTAPAESSDNAHIVWIGPVEDIQRREDLWRRIVSGDRSERF"
     gene            13053..14852
                     /locus_tag="VHV99_16180"
     CDS             13053..14852
                     /locus_tag="VHV99_16180"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX3636993.1"
                     /translation="MMRPGLTIKLSILLACIGVLASGATGYYAYRANRTMLVNEAESS
                     LLTSTELLGQRFSGAIDDVGADALVLASLPSSAHVAQTDDASGVNASRERLAQVYASF
                     MVHHPEYLQIRLITRQHYGLELIRFDRDADGLVRVEGRNLQEKGQFAYVFDTLAFSPG
                     RIYTSPISVNHEYGAHAAEGKPTLRLGTPVVSASGAVVGVIVIDIDLAGLLKRLQSDL
                     PSDYQVYLANEWGDFLVHPDASKTFGFDKGRRFLMQDSFSVTRPLFEQSQSEELVNGL
                     AHPKQAAGQVLAFVRRPFGASEGNRFIVLGLAKPLDDVLSGAHLLGFSIIRMVMIFSV
                     LALFLAILFARALTRPLHSLAYAATHLFAEHAMETLPLKRTDEIGVLARCFDRLRREI
                     KSQMDVLHGKQRELVHLATHDGLTGLPNRMLFMQKLEVAIEEATRRKERLAVLFVDLD
                     CFKQINDQFGHSIGDKVLAAVAQRLKRVLCTADLAARLGGDEFIVLIEGPRSAEAAPG
                     IAARIMGTLNEELSVDGQSMTVGASIGISQFPDDSATAEELLLHADAAMYAAKSGGRC
                     AYLRYRDVLEARELKQSQAGEGATEGREAEPTA"
     gene            complement(15002..15448)
                     /locus_tag="VHV99_16185"
     CDS             complement(15002..15448)
                     /locus_tag="VHV99_16185"
                     /inference="COORDINATES: protein motif:HMM:NF019736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636994.1"
                     /translation="MVGIGKAANSSEGLERYANPALKHRCAQIAMDGSQKIPPRPLAT
                     ISARLAAGQPFTRLALAVAAWMMFLYGHADDGTGYEISDPLASRLKTLAASAHGETQA
                     LMDALLSVCEVFPADLVALPAFRKALHQALLLLQAKGARGAIEASQ"
     gene            15438..16271
                     /locus_tag="VHV99_16190"
     CDS             15438..16271
                     /locus_tag="VHV99_16190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX3636995.1"
                     /translation="MPTKNLRANASSFSSDATRVAPRTRRRASVQAGARVELPDLTAS
                     VSFDSHEPIGKQIFRALRQAIFVGQLVPGTPLSEKEVSDMFQVSRQPVREAFIKLVEA
                     GVLQVFPQRGTFVKRISPRQVREGRFIREAIETAVVRKAAVSITDQQLQALADNLRDQ
                     KLAAKSNDTAAFLALDEAFHYAIAQAIDCTAAWETIQDIKAQMDRVRYLSLPDVSPLD
                     LLIKQHAKILAGLRAHDPSAAEEAMRSHLREILMSLGPIAERNPAWFEADEPERVPLS
                     T"
     gene            16371..16709
                     /locus_tag="VHV99_16195"
     CDS             16371..16709
                     /locus_tag="VHV99_16195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3636996.1"
                     /translation="MYDAEIAATLLNRWATRSSATDFATYLELLREGNLSFTYQSGHV
                     REVGIEAGIEAGIEAGVESGTEAGSAINIESLVFGDGSRTLRVEAPDRTPRWTRWAAV
                     EPLLPAPSET"
     gene            complement(16722..17855)
                     /locus_tag="VHV99_16200"
     CDS             complement(16722..17855)
                     /locus_tag="VHV99_16200"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HEX3636997.1"
                     /translation="MLIRNARIVTRDEVFTGVVRIEDGVIRDVERGTTSAREAEDWDG
                     DFLLPGLIELHTDNLEKHLVPRPGVQWNTDAAFVIHDAQVAAAGITTVFDALSIGSRS
                     KVGLRGRDQQTQCAESLTRFSERKLLRAEHFLHLRCEIATADVVEMFDSLCAHPLLRL
                     ASVMDHTPGQRQWHDRDQWRRFQERNGKLTDEHVATALVELSVEQERYADAHRREIVA
                     RCKQLGIPVASHDDTLIEHVEQAKAEGIVLAEFPTTRIAAEAAREHGISTIMGAPNIV
                     RGGSHSGNVSALELAKADLLDILSSDYVPSSLMTAVFELVSKAGWTLPRAMATVSAEP
                     ARTAGLHDRGAIETGLRADFVRVTMLDTLPVPRATYRAGVRIV"
     gene            complement(17950..18717)
                     /gene="phnL"
                     /locus_tag="VHV99_16205"
     CDS             complement(17950..18717)
                     /gene="phnL"
                     /locus_tag="VHV99_16205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HEX3636998.1"
                     /translation="MRSIETSKESHAAERAFIENNALMLRAVGIGKTFTLHGQGGVQI
                     EALAGVSLDVERGECVVLVGPSGAGKSTLLRCLYGNYLASTGSIAIRDAAADDGQPVS
                     ITGAEPHDVLRLRRGVVGYVSQFLRVIPRVTTLTLVAEPLLSRGVAEDEARARAAALL
                     ARLNVPERLWSLAPATFSGGEQQRVNIARGLIAGHPLLLLDEPTASLDAENRDVVADL
                     IVEARERGAAIVGIFHDEDTRNKVATRRLELQPPLRH"
     gene            complement(18726..19496)
                     /gene="phnK"
                     /locus_tag="VHV99_16210"
     CDS             complement(18726..19496)
                     /gene="phnK"
                     /locus_tag="VHV99_16210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HEX3636999.1"
                     /translation="MTPLLSARSLTKQFGGRNGCRNVSFDLYPGEVLCIVGESGSGKT
                     TLLNTLALKSAADSGSLHYTATHGEKLDLLALSEPRRRLLMRTEWGFVQQNPRDGLRS
                     GVSAGANIGEPLMAVGARHYGDIRHTATQWMERVELDATRIDEFPAAFSGGMQQRLQI
                     ARNLVTGPRLVFMDEPTAGLDVSVQARLLDLLRTLTSTLHLSVLIVTHDIGVARLLAH
                     RLMVMQGGQVVESGLTDQVLDDPQHPYTQTLVSSVLPV"
     gene            complement(19493..20416)
                     /locus_tag="VHV99_16215"
     CDS             complement(19493..20416)
                     /locus_tag="VHV99_16215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HEX3637000.1"
                     /translation="MNAPDISLATASYDSAADGYNFAYLDEQTKRMLRRALLKAVAVP
                     GYQVPFASREMPLPYGWGTGGIQVTAAIIGKNDTLKVIDQGSDETTNAVNIRRFFART
                     TGVATTRRTTEATIVQTRHRIPEAPLTEKQILVYQVPMPEPLYKLEPRATECKKLHAL
                     ADYGLISVKLYEDIVHHGSIATTYDYPVIVNHRYLTSPSPIPKFDNPKMHMNPALQLF
                     GAGRERRIHAIPPYTPVRSLDFDDHPFEVQKWQHACALCGSTESFLDEMIVDDAGKRM
                     FVCSDSDYCHERRGDQDLELPSYEARKGETA"
     gene            complement(20413..21558)
                     /locus_tag="VHV99_16220"
     CDS             complement(20413..21558)
                     /locus_tag="VHV99_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HEX3637001.1"
                     /translation="MYVAVKGGERAIEASWRLLDKARRGDTRLAELSVAQIREQLRLA
                     VARVMTEGSVYDEELAALAIKQAAGDLVEAIFLLRAYRTTLPRFGYTQAIDTEAMQVE
                     RRISATFKDVPGGQLLGATYDYTQRLLDFALLAEGDGDSGIDAAATTSSIQTAQAAAE
                     AMPRVVTLLDKEGLIEQERPTPNAPEPGDLSREPLAFPASRTTRLQNLARGDEGFLLA
                     MGYATQRGYAHTHPFAGEIRFGAIAVEMELDELGEAVEIGEIDITECQMINQFAGSGS
                     VPPAFTQGYGLAFGHSERKAMAMALVDRALRAEELGETLASPTQDIEFMLSHSDNVEA
                     SGFVQHLKLPHYVDFQSELELVRRLRAQHGAADKNQGQDQDQQEQAA"
     gene            complement(21558..>21776)
                     /locus_tag="VHV99_16225"
     CDS             complement(21558..>21776)
                     /locus_tag="VHV99_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HEX3637002.1"
                     /translation="MIRVDALTGGAPVVLSGPCIQHTATIAPVGLPEHFWRERAALAP
                     LFPCGVDCYLVCGNTLIGLPRTTQAKVN"
BASE COUNT         4104 a         6652 c         6865 g         4155 t
ORIGIN      
        1 ggaatcatgc cgtcgaagtc gttttcgacc cacacgcact tggccttcag gcgtgcgcag
       61 atttcattgc cgaggtcgat atcgaagcca acgagcttgc cgtccgaacc cttcgactca
      121 aacggggggt agctggcgtc aacgccgaaa cggatagtcg accaatcttt agcgtgtgcg
      181 ccaatcgaga cggtggcaag cagagcaacc gtcaaagccg ctagcagttt tttcactgtt
      241 taactcctcg gtgtggttca aatactcccg tatgcggttg tgccgctgcg ggatgcccgg
      301 cgcgactcac gaccaggctt gggttgaagc tgcgggccgg gccggccagc agcgcgcgcc
      361 aagcttatca gttcaaaaag aacgggtagc tagtgaaaca ccggatggcg cacgggaatc
      421 gcttttatat tgtccgcatg gcggcaacgt caaacccgcc gtggacggct tcagaatctg
      481 cttcagaagc gttcgagata gtgttgtgca gcgcgcatca catgccgctc ggcgaggagt
      541 gccgcgagcg tcgcgtcgcc ggattgcacc gcgcgcagga tggcggcgtg ctcttcccag
      601 gtggtgcgta cttcggcgtc gttggcgttg acgaacagca tgcgggtgcg gtcgcgcagt
      661 gagcgcatca gatcggacag catcgaatta gcacccgccg agtacagcag atcgtgaaag
      721 cgggtattca attcgagcag gccggcggtt tcaccggccg ctaccttgac gttgccttcc
      781 tgcaatacgc gctcgatctg cctgacgagt tccggggtgc agtgctcggc agcgaggcgg
      841 gcattgagcc cttccagcgt ggcgcgaatc tgcaccatct cgcgcgccgc ggacgggtcg
      901 agcgatgcca cgatcgcacc atggcgcggc cggacctcga cgagaccttc cgatgccagc
      961 gcgcgcaacg cctcgcgcac gggcactcgc gacacgttga gttcctccgc gatgcggcct
     1021 tcgaccagac ggtcgccggg cgcataggcg ccagacgtga tggattcccg caaacgggcg
     1081 atgacgagtt ccgacaactg cttatgcagt atcggttcgc gcccgctcgc gccagaggct
     1141 tcccgctcgc tactcattcc atcccccgta aaatggcctt gcaacaacca tgcaataggc
     1201 cacgcaatag ccatgcatct gatgcgccga ttttaccgca taggttggat tcagtagact
     1261 gtagacgtca tacagaagcg gtttttgtta gtaagggact cgcgagcggg ttcattgtcc
     1321 ttccggacgg cgcctgggtc gtcaccgagg agttaccacg agacgctgac gtcaccgttg
     1381 acgaacgtct gacaggccat ccggtagccc gcttcaagct cttgtgcgct gagatggcgc
     1441 agttccttct gtttcaccgt gtcggtattt tcacgtcctt cctcgagcct gcatttgcac
     1501 gtgccgcaca ggccaccgcc gcatttgaaa gggatgccgc cttgctcgcg caacgagacg
     1561 cgcagcaggt tgctgttggc cggcgcttga acagttttgc cgccgttagt cagaaatgtg
     1621 atttgaatca tcgaaagcct gtcaatcaaa tgcggttttg ccgtgttcag ccgaagtatt
     1681 tcgctcgact aactgtgcgt cggcttgcgt gacgcccgcg agctcctgga tcgagatcaa
     1741 ggcggcctca cgtgtggaaa atttctcgaa gaacctggtg ggcaccaggt tcactgtggg
     1801 cggcgaggac tcgtcgacga tcctcactct gctgtctttc ccgattgaag caagcacgaa
     1861 agtggcgatg tgacgatcgc aatagaagta gtcataggcg ccgaccggcg tgatgccgtc
     1921 cgttgcttcg ctacggtatt gcccgggttt gctggtcagg atgatgtaca tgagtgtctt
     1981 ttggaaaatg cgattcattg cgcgtccccg gcttcgtcct gcgcgagttc gacgtcgtgt
     2041 tcgatccaga gctgacaggc gagccggtaa ccggcgttca gccgttcccc gagttgcttc
     2101 ttctctttcc agttgggcgg cggcagttgc tccgcgccgg cgatgacccg gcaggcgcat
     2161 ctggaacact tgcccatgcc gcactcatag cgcagatgcg gaaagggaaa ttgacggatg
     2221 ccggcacgca caacgagatt ggtgttctcc ttgacggtgt ctcggtacac ctcgccgttt
     2281 ttgtggaaca cgacggtggg catgggttgg ctcctgggaa gctgggcaat atatgtacat
     2341 cgtatacatt ttggaaaacg ttcgcgggct gttttctgac ctttgtttaa tctcggcgct
     2401 cccgcatctg atgctggagt tgactaaagt ataccgtata cttaaattgt gcggtgtgca
     2461 ttgtatcgac cgcgttacgc ggtcatcgcc gcaaacccga agccttagcc atccgattcg
     2521 acaaagctga caaggaacca ccactcatga ttcatttgaa cgatcaacag atcgacgaac
     2581 tgatcgatct ggagcaagcc attcccgccc ttgacgacgc gttccggcaa ctcggccgag
     2641 gcgaggccgc ggtacaacgc cgcgtcagaa ccgaagcaaa cggcgtaaaa ctgtcgacgc
     2701 tcggcgcggt tctcccgggc gccgaggtcg tgggcgcgaa ggtctacacg acgatcgccg
     2761 gcaaattcaa cttctcgatc gtgcttttct ccacctcgga cggcagcctg ctggccacac
     2821 tcgatgcgaa tgcgctgacg cgtctgcgca cggcggccac cacggcgatc gcggtacgcg
     2881 cgctcgcgcg accggccgcc acgagcgcgg caatcttcgg cacgggcgtg cagggcacag
     2941 cgcacgcgca tgcgcttgca aggttcacga atctgcaatc gctccatatc gtcggactag
     3001 agggcagcga cgcgctcgcg gcggagctga acgcccgcta tggggcgcgg ggctttagtg
     3061 cacaggcctc gcaggcgcag gcggcagtcg agcaagccga cgtcattgtt acggcaacgc
     3121 gcgcaacggg gccgttattc gatggccacg ccgtgaagcc gggcgcgttg atcgcagcaa
     3181 tcggctccag cttgccgacc acgcgcgaac tcgacgacgc gacgattgcg cgggcctcgc
     3241 gcatcgtcgt tgaattgcgc gagcaggcga gggaagaggc gggcgatctc gtgcaggccg
     3301 cgccgggtat cgtcgactgg acggccgtgg tggaactggg cgacgtgctc gaaggaaaag
     3361 cccccggccg cgcgcgcgac gaagacgtca tcgtctataa ggccgtcggt atcggtttgc
     3421 aggatgtggc gctggccggt ctcgcgtatc gcacctacct cgcgagccgc tgattcggac
     3481 gctcagatgc tcagatgccg cgccgagggg cgcggcatca tatgacgcga agcaaatgcg
     3541 tcagcgccaa tgcgtcagct ttcgcactgg cgccgcatgc cgaggcgacc ggcaagcgga
     3601 accaccgcgg cgacgagcac acacaatgcg atccgtagcg cgttgccacc ggcggtgtcg
     3661 ccggctacgg cggcccacgt ctgctgcagt gcgccgctga cctgcgaatc cgccagcagc
     3721 atctctccag cgatccatcc gaggagtgcc gctcccgcat aagtgagcag cggaaacttg
     3781 ccgagaagtt tcagcatgag tgtgctgccc aatgcaatca ccggcgcgcc ggccgcgagt
     3841 ccggccagca ccaccaggat gcgtgacgat tccggcagcg cttcggcgat cgccgccgtt
     3901 gcaaggccgt tgtccagact catcgagaga tcggcgaaca gaatcacgac gatggcgcgc
     3961 cacagagccg tcgaacggcc cgcggaggtt tcgccgccgc cgtcatcggt tcgcagcagc
     4021 tggataccga tccacaccag caatagcgcc gctatcacgt gcatgtaggg gatcgcgagc
     4081 agaaagccga tcaggtagat catcgcgagg cgtaatacga tgcccagact cgtgccgatc
     4141 cacacgccga gccgccgctg ccttgccggc agcgaacggc atgccatcgc gatggcgatc
     4201 gcgttgtctc cgccgagcag tacgttgatg aagacgatct gcaggatcgc cgtgccctca
     4261 tgcacaagtt gcggggaaac gaagttcagt tccatgccgg tgtgaacgct caactgatcg
     4321 aaaccgcgac cagcgtggcc accaccaggc cgcatacgac aggcaggaac agtacacgca
     4381 cggcctcgag gacgggtacc cgcgcgaagc ccgttaccgc aatcagcgac gaccacgcga
     4441 ccagcgtgcc accacccgtc cagaccgcgc ccatctgccc gatcgcggcg agcgtggcgg
     4501 ccggcatatg gaccatcggc gccaatgcac ccgacaacga tcccgtcagt ggcaagccgg
     4561 cgaaccccga accgtcgatg ccggtaatca tgcccatcag cagaacgccg aaggaaacga
     4621 acaatgggct cttcgggatc aactggctac aggcctgcac gagttcgaac aggaacgacg
     4681 gcacgtgcgc accgggggcg atgccgagaa tggaaccggc cgtactgctg tcgccgacga
     4741 agaagaagcc cgcaatcggc agcacggcgc ccattgcctt gaaggcaaac accaggccgt
     4801 cgatgatgtg ctcggcggtc cggtccagaa agcctttgcg gccatgcgaa agcgtcgcga
     4861 gcatcatcag cacggctgcc atgccgccga tcaaggcggc agcgtcgccg ccgcgtatga
     4921 cttccgcgcc gatcatgtgt ggacttgtca tcaccacgac gaccgcgagg aatgccaacg
     4981 gtgtaacgac cgagaacact ttcgaccaca tctcgcggcg atccagcggc gcggccggcg
     5041 cgatgccgct taaaggcagc gccgctgccg cgctggcggc gagcggcgcg tgaaacagct
     5101 cgcctggcgc gccgctggtg ccgcgcgcca gctcgacctt cgcgaaactg ccctggtgct
     5161 cctcggctgg tgagacgacc gccgccacgc cagtttccca cgcgatcaga ttcgcggtgc
     5221 cgggcgcctt cacgttcttg cggaccagga acaggtacgt cagaccgagc gcgacggcgc
     5281 cgaccacgat cgacaaagtg aaccccatgt tcgcgaccga caacatgtcg gcgcccgccg
     5341 ccttcgcgct aatcccaggc gccaccttga tgacgtaatc ggacgaaagc gccatgcctt
     5401 ggcccgcaat ggcgatcgcg gccgcggcgc cgagcggcgg cagtccggca tacacggcgg
     5461 ccggaatcag aatggcgccg accagcggca cggcgggcgt gggccagaag aacagtgaaa
     5521 tcaggtaagt aatgagcgcg agaatgaaga acgcgacatg accgttcacc atgatccgtt
     5581 tgaacggttt caccatccgc acatcggccc caagttcgcg caagccgttg agtagcgcgg
     5641 tcatcagcgc aatgacgaga aagatgttga agagctcgtg cgccgcggtc aggctgccca
     5701 tgaagatggc ctgaagaccg actagtagtt tccccgagaa ggcgaacgcg accgcgaacg
     5761 tggcgaggat ggctggaacg acgacattgg cacggaaaat catggtcgcg ataatcgcga
     5821 ccacgcccac gagataacaa tagtgggcta gtgtgagtga tgcaacgggg tgcatgaaat
     5881 cctcggctcg gtatgtgggg gcgatggatg ggagcttttt ttgccagact ataatcacca
     5941 acggttttta aaaaaaaatc tgcggtactt tttatgcacc gttcgcgcgg ccaaacattt
     6001 catttggtag ggatcgcgaa atggtccgca cgcgtgaact gtgtcaatga caggcgccga
     6061 tgagatcgct tccggacctc aggagccacc cggtaaaagg gcggcggcgc actgcaccaa
     6121 cgtctaaagc ccggcgggtg acgcctgtgc gacgccagcg tccgcgtggt ggaagccgaa
     6181 gccatccgaa tagaagtaac gcacgctcgc gccgtgttcg acaaagacac gcttcgcgtc
     6241 ggcgatcatg tccggtgaac cacacaggta gagcgcgtgt tccgacaggt cgggataggc
     6301 cgcgacgacc gcctgctgca cgtagccccg tgagccgtcc cacgtgtcgt cggcgcgtga
     6361 cagcaccggc gtgtacgtga agtcgggata gcgcgtggcg agttccgtga gggcgtcgtg
     6421 caaatacaga tcggcgggcg cgcgtgcgcc ccagaatagc gccacgggcg ggcaacccgg
     6481 atcgggcagc atttgctcga gcatgctctt gagcggtgcg atgcccgtgc cggtcgcgac
     6541 catcacaagc gggcgaaagt cctcttcgtg ataaccaaaa ccgccgagcg gcagttcaac
     6601 gtcgagttga tcgccgaccc gcaggttggc aagcagttgg cccgtgaaat agccgccctg
     6661 aatacgacga atgtgaaagt ccgctgtggg cgcgccggga ctggatgcca tcgaaaagct
     6721 tctggtgcct gcctcgctta acagcaccct catgtactgg ccggggcgaa agtcgaacgg
     6781 cgcggtgccc ttcaccgaga gcgtgagttt gaccacgtca tgagcgagcg gctccagcgc
     6841 ttcgaccgtc gcgcagtggc gagtgggcgc gacgggcgag gcgagcggcc ggtcggtgct
     6901 gatacgcaga tcggcactgg gccgtgcctg acaggcaagc gcatagccgg cgacttcttc
     6961 ttccggcgcg aggcccagcg gttgctcgtc gtactgcacg cgtccctcga cgagtttcac
     7021 gcggcacgta ccgcagccgc cgaaccggca ttccgacggc agcggcacgc ccgcgcgcag
     7081 cgcggcgtca agcacggttt ccgacgaaga agcctcgaag ctgcggcctt cctcgatcca
     7141 tgtcacctga tagttcacaa tgccgcccct cgcgtttaaa gcttgatgtc gctctgaacc
     7201 agcacttcgc ggttcgcgtc gcgtagcaga tcccgcaagg cggcaagtcc ggcgagaccg
     7261 gcggcagtgt cctgttcgcc gttgccgccg gacaggccaa tgccgcctac cacctggttg
     7321 tcgaccacga tcggaaagcc gccgacaaac gccgcgaatt ttccctcgaa gctccactga
     7381 ataccgaaag cttcattgcc aggcagcgcc ggaccgttgg gcggcgtggt gaaaaggtga
     7441 gtagagcgct tgtggccggc ggcggtgaat gccttgttcc aggcgatctg tggaccggta
     7501 atgcggcctc cgtccatccg ttcgagagca agcggaaagc cgccttcatc gacgacacag
     7561 acggactcga gcacgccgat ctccgtggaa cgggcaatgg cggcctggat catatggcga
     7621 gcttcagcga gctcgagttt gaaaaatgct ttcatggttt gcgaatgtga agaggttgtc
     7681 ggtaatgcgc ttacacgccg cgatagacgg tcttggtctg cagatagaac gccagacctt
     7741 cgcggcccga ttcacgaaac gtcgcggtgc ttgaattttt cagtccgccg aaaggtgcat
     7801 tgatcagatt gccggtggtg gtgcggttga tcttcacggt gccggccgcg atgtcttccg
     7861 caaagcgatg catcagtttc gggtcgcgcg tgacgagggc ggcggacagc ccgtattcgg
     7921 tatcgtttgc cactgagatg gcgtcggcat agtcggcgac ttcgatgatc gcaatgaccg
     7981 gaccgaagat ttcctcgcgc gcgatgcggg cggttcgcgc gacgtcggca aacaccgttg
     8041 gctcgacgaa aaagccgtgg ccaaagtcgc cgcttgtaat gcggttgccg ccggtcacga
     8101 gcgtcgcttc gcgtttgccg atttcgatgt aatcgagcac ggtacgcaat tggccttctg
     8161 tggcgagcgg gccgatctcg gtgccgggcg tcatgccatt gccgattttc agcgccctgg
     8221 ctttttcaac cagctgtgcg atgaaagcgt gcttcacctg tggggtaacc agcacgcggc
     8281 tcgtgccggt gcatgcctgg ccggtcaggg aaaagccgcc tttcacggtg agatcgacgg
     8341 cgcgatccac gtccgcatcg tcgagcacga tcagcggatt cttgccgcct agctccatct
     8401 gggtacgcgt cgacatggcg gcggcgcggt gaatctgttc gccggcagac gtcgaacccg
     8461 tgaatgaaat cgcgcggatg tccggcgccg cgacgataac cggtcccacc tcgctcgatt
     8521 tgcccgtcac gaagttcagg acgcctttcg gaatgccggc ttccacgaag gcttcggtca
     8581 ggcgaaggcc gctgagcggt gcatcggacg acggtttgaa caccacggta ttgccgcaga
     8641 caagcgccgg cgcgatttta cgagcgggga tcgaaacagg aaaattccac ggcgaaatca
     8701 ccgtcaccac gccgagcggc tcgcgcaacg tgtagacggc catgtccggg tcgtcgttag
     8761 gaaaggtttc gccgccgaac gactgcgcct cgatcgcgta atagcgaatg gtttgtgcgg
     8821 agcgcaggaa ctcgtctttc gcgaggtttt tctgcttgcc ttcttcgcgc gtcagttctt
     8881 ccgcgaatcg ctctgcgtgc tgctcgagat acgtcgccgc attcagcaga atcttcgagc
     8941 gtgccgaggg cgacagtttt ttccactgcg cgaatgcgcc cgccgccgct ttgacggcgg
     9001 ctgtcgcatc ggcggcactg gaggcctgaa agcggcccac cacgtcacgc gtgtcggcgg
     9061 gattgatatt gtcgaaggtc gcgccgctca ggcttgcgca ccattcgccg tcaatgtagt
     9121 tcagatacgt ttgaacgttt gtcatgaaat accggccctg caagaaaaga agcaaatgcc
     9181 cgccgtcgac gccgtagcgc tatgccgacg accttggcgg gtcgttgtgc tgtatcgagt
     9241 gcccgagctc aggcggccat gccgagttca ggaagcggga tttccttttc gacgtagtcg
     9301 taatagagcg cagtcgtata gagcaggcgc atctgcgcac cgacctcgca gatcttcagg
     9361 caacgttgct gcaatgccgg ggtgttcgcg tgttcgagca cgatctggta gccacgctcg
     9421 ccgtggattt cgtcggagac gatgtgcaga tcgaagaact cgacttcttc gtcggtgaat
     9481 ttgtattgct cccgcagcgt cggcgtctgc ttgcggtaga tcgacggcac ctgcgattcc
     9541 aggccgacca cgagcgccgc gaccgcgacc accggctctt cgcgcattgc cacggaatag
     9601 caccagccct gcaagccgcg cgtggtaggc gacatgttgt cgggatcgac aacgcgctca
     9661 cgcgtggtgc cgcaggcttc ggcgaagcgg atcagcagat ccgtgtgacg atcgccgccg
     9721 atctcttctt cgtacatgtt cgccagcagg aagtccttcg cctcggtcat gtgatcgggc
     9781 gtgcgcgcgt acagatagcc gaggtaatcg gcgaacgggc cgacgtagtg gtaatggttt
     9841 tcagcccagc gcgcgagatg agtgcgcgaa agcttgccgc tcgcccatgc cacgctgaag
     9901 ggcgcctggt tggcgctctt gcccttgatt gcctgttcga gcgccgcgcg aaacgcgtcg
     9961 cggttcatca gttctgccat gtctgactcc ttcgattggt gggatatctg cgcttgaaat
    10021 catgcgacgc agatatcagt acactgtata cagaaatttc cgatatgcca agtcgcgaaa
    10081 aaagtctttg ctgccgttag aagctgtggc gcagcccgat gccgagcgcg ccctgttcgt
    10141 tattgctgga ggcagcgatg aattgcgtca cctgggcgaa ctgtgcatca cgcaaggcgc
    10201 gctggaaggt cgcgaagccg tacagttgcg tacgcttgga cagcgagtag gtgagcatgg
    10261 cgttgtactg gaggtagcgc gggtcggcat tgttcgcatc gacctgaccg tccgtgtagg
    10321 tcaaacccag cccggcggag aaggccggcg tgaagttgta aagggcattg acctcgtaat
    10381 tcatgaagtc gacgttctgg ccatcgaaac cttggttgta gctcacgctg gtgatgtcgg
    10441 cgctgatctt cgccgagccg atcgtatacg agccgccgcc gccgaccgtc aggaatgaat
    10501 gggctgccag cgcgtaggtg ccgaccgcag agttggtcga tttccagaat gtgcccggcg
    10561 acagatcgcc tggctgtctc gcgtaggtca tgccgaagcc gagatacacg gggccgttcg
    10621 catagctgcc gccgaggctc cacatgctgt ctttgccgaa actgccgggc tggccgccga
    10681 agctgtacat cgcgctgccg gtgaagccgc cgaagctgtt gccgtagcgc accgagttgt
    10741 tgactcgcac gccgcgattg gtcaggtcca tgtcgccggg gtgttccatg tacacggacc
    10801 agtcggcggc ggtcagttgc gcgacataat cgaccatcga ttcatattgc cggccgagcg
    10861 tcagccgccc atagcggtcg ttctcgatgc cgacgtaggc ctgccgcccg aacatcagcc
    10921 cgccttggcc aagggagccg tccgtgatgt cgaagccgtt ttcgaggcgg aatatcgcgc
    10981 gcgtgccgcc gccgaggtcc tccgagccga cgaagccgac ccggcttccc cgcgtggcgc
    11041 cgctgcccat ggcccacgtt tttccgccgc cctggttggt cgtataagcg accgatgcgt
    11101 cgatgagacc atatagcgtg acgctgccct gagcgtgagc ggtgaccgag agggaggcaa
    11161 gcgcggcggc ggtgcagata ctctttttca tcaaagtcgt tcctgttatg ggctctcgcc
    11221 gttcccgggc gagggcgaag cgttgcacga ggggagtgaa tccatatcag tatgctgtat
    11281 acaaaaatac gaaacgcttt tttggaatga aaatctgctc gtctttccaa tcgaagttct
    11341 tgcgcgaaaa ggtgccgcaa taacgcgcgg attgcgtatc gacgagccga cgtccgaacc
    11401 gacgtcgaag aacgacatgc cggcgcacac cgcggccgcg ctgccgcctg tgaaccgcca
    11461 ggtgtcctcg caaggtcgcg tggattgcgc gtggtgccgt aaatgtcgtt gtagctttgc
    11521 cagtcacgca gatagattgg cacgttggtt ttgccgggca gcacggcgcc ggcgtttgtc
    11581 aatcgctgca aagaggcgag caacgctttc ggtccgcgga acccgcattt cgaatgtttc
    11641 ctcgacccat tcagttaaag tttccgcgaa atcgaccgtt atcaaggtca acgcgagatg
    11701 agcgcgttgg tatatgccag cagcctattc ctgccgaccc cggcaaggct caaccaaaaa
    11761 cgccctgcgg agcgctatca tcaacacgct gcgcgcaagc cgcgcagctc aagcgatatc
    11821 tgtctgaaca aagtcatcga gtactgcgcg cgcgggtacg cgccgcgcgc ctgtctgcgc
    11881 tcgtcgtagc agccttctgc taagtggtgt tatatgcacg tgatttttac agcagatgaa
    11941 aagtgcggtt tcaaagcgcc gttccggcgc cgctttgcag cggctgcggc tcgcggaagt
    12001 tcgctacgcc gcagcggttt tttgacccta ctgataagcg ccgttttctg ctgcagcctt
    12061 ttatcgtcag ggccggttgc ggccgccgaa aaaatcctgc gtgtactggc gtggcccggt
    12121 tatgcggatg ccgacgtggt gaagactttc gaagcacgct ataacgcgaa ggtcgaagtc
    12181 acgctggtcg attccgacga ggccctgtgg gcgcagatgc atacccagag tacgccgcca
    12241 ttcgatgtgc tcgccgctaa taccgccgaa atccagcgtt atacccgcgc gaatctgctg
    12301 gcgccgctcg atctcgccag cctgccgaat accagaaggc agatgccgcg gtttcaggca
    12361 ttgtcgtcga tcgaagggtt gacctcggca ggcaaggtgt acgccattcc attcacgtat
    12421 tcgtcgatgg gactgatcta tgaccgtaag cagatcgccg ttgcgccgcg ctcgatgcgc
    12481 gaattgtgga acccgcgcta ccgcggcaag gtgctcgact tcaacagcgc ccaacctaat
    12541 ttctcgttca ccgcactggc gctgggctac cggcatccgt tccagttgga cgccgcgcag
    12601 atgcgtgtga tcactcaaaa gctcgtcgat ttgcgccgca acctgttgac ctattacacg
    12661 ttgccggaag aagcgaccgc gtttttcatc cagcataaag tggctttgat gttcggtaac
    12721 tatggcaccc agcaggttga atcgctgcgc cgcgccggcg cggacgtggg ctacgtgatt
    12781 cccgacgaag gcgcgctcgc gtggctggac tgctggtcga tgacgcgctc ggcaaccgat
    12841 cgcccgctcg cgctggcgtg gatcaattac atgctcgagc cggacgtcag tgccttgttg
    12901 acgcaacggc aggggctcgc caacacgttg acggcgccgg cggaaagcag cgacaacgcg
    12961 cacatcgtct ggatcggacc ggtggaagat attcagcggc gcgaagacct gtggcgcaga
    13021 atcgtatccg gcgaccgctc ggagcgtttc tgatgatgcg tcccggcctg acaatcaagt
    13081 tatcgatcct gctcgcgtgc atcggcgtgc tcgcgtccgg cgcgaccggt tattacgcgt
    13141 atcgcgcgaa ccgcacgatg ctcgtgaacg aagccgaaag cagcctgctg acttcaacgg
    13201 aattgctggg ccagcgtttt tccggggcga tcgacgacgt cggcgcggac gcgctggtgc
    13261 tcgcgagctt gccttcttcg gcgcacgtcg cgcagaccga cgacgcttct ggcgtgaatg
    13321 cgtcgcgcga acggctggcg caggtgtatg ccagcttcat ggttcaccac cccgaatacc
    13381 tgcaaatccg tttgattacc cgtcagcact acggactcga gctgattcgt ttcgaccgtg
    13441 acgcggacgg ccttgtgcgg gtcgaaggac ggaatttgca ggaaaaaggc cagttcgcgt
    13501 atgtgttcga cacgctggcg ttctcgcccg gtcgcattta cacctcgccg atctcggtca
    13561 atcacgagta tggcgctcat gccgccgaag gcaaaccgac gctgcggctc ggcacgccgg
    13621 tggtgagcgc gagtggtgcg gtggtgggcg tcatcgttat cgacatcgat ctggccggct
    13681 tgctcaaacg tctgcagagc gatttgccaa gcgactatca ggtctatctg gccaacgaat
    13741 ggggtgactt tctggtccat ccggatgcct cgaaaacatt cggcttcgac aaagggcggc
    13801 gcttcctgat gcaggacagc ttctctgtca ccaggccgct gtttgagcaa tcgcaaagcg
    13861 aagagctggt gaacggtctt gcgcacccga agcaggcggc cggtcaggtg ctcgctttcg
    13921 tacgcagacc gttcggcgcg tcggaaggca atcgctttat cgtgctcggc ctcgccaagc
    13981 cgctcgatga tgtattgtcc ggcgcccacc tgctgggttt cagcatcatt cgcatggtga
    14041 tgattttcag cgtgttggcg ctgttcctcg cgattctgtt cgcccgtgcg ctgaccaggc
    14101 cgttgcacag tctcgcgtac gcggccacgc atctgttcgc cgagcacgcg atggaaacgc
    14161 tgccgctcaa acgtaccgac gaaattggcg tgctggcgcg ctgcttcgat cgtctgcgcc
    14221 gggaaatcaa atcgcaaatg gacgtgctgc atggcaagca gcgcgagctc gtgcatctcg
    14281 ccacgcacga cgggctgacc ggcctgccga accgcatgtt gttcatgcag aagctcgagg
    14341 tggcgatcga ggaagcgacg cggcgtaagg agcggctcgc cgtcctgttc gtcgacctcg
    14401 actgcttcaa gcagatcaac gatcagttcg gtcattcgat tggtgacaag gtgctggccg
    14461 ctgtggcgca acggctcaag cgcgtgcttt gcacggcgga tctcgctgcg cgcctcggcg
    14521 gtgacgagtt catcgtgttg atcgagggac cgcgttccgc ggaagccgca ccggggattg
    14581 ccgcgcggat catggggacg ttgaatgaag aactgagcgt cgacgggcaa agcatgacgg
    14641 tgggtgcgag cattggcatc agtcagtttc cggacgacag cgccacggcg gaggaactgt
    14701 tgctccacgc cgacgctgcg atgtatgcgg ccaaatccgg tgggcggtgt gcgtatttac
    14761 gctaccggga cgtgcttgag gcgcgggagt tgaagcagtc gcaggcgggc gaaggggcaa
    14821 cggaaggccg cgaggcggag ccgactgcgt gaagcggcga tgagcggcgt ctgataaaag
    14881 cgcctgataa aagcacctga taaaagcacc caccagaagc ggtcatccga agccccaacc
    14941 ggatcttcca actcgacgct tcctgccgag cccaccgcgc tcgcttccca aacgcgggga
    15001 ttcattgcga cgcctctatg gctccccgtg caccctttgc ctgaagcaac aacaacgctt
    15061 gatgaagtgc ttttctgaac gccggaagcg cgaccagatc tgccggaaac acttcgcaca
    15121 ccgacagtaa cgcatccatt agcgcctgcg tttcgccatg cgccgacgcc gcgagcgtct
    15181 tcaacctgct ggccagcgga tcgctgattt catagcccgt gccgtcgtca gcatggccgt
    15241 acaaaaacat catccatgcc gctaccgcca gcgcgagacg tgtgaacggc tggcccgccg
    15301 cgagcctcgc cgagatcgtg gcgagcggac gcggcggaat cttctggctg ccgtccatcg
    15361 caatctgcgc acagcgatgt ttcagcgccg gattcgcgta gcgctccagc ccttcgctgc
    15421 tattcgctgc tttgccgatg ccgaccaaga acctccgcgc caacgcgtct tctttctcct
    15481 ccgacgccac gcgcgtcgcg ccccgcacac ggcgccgcgc gagcgtacag gccggcgcgc
    15541 gcgtcgaact gccggatctg accgctagcg tctcgttcga ttcgcacgag ccgatcggca
    15601 agcagatttt ccgtgcgctg cgtcaggcga ttttcgtcgg ccagctggtg ccaggcacgc
    15661 cgctctcgga gaaggaggtg tcggacatgt ttcaggtgtc gcggcagccg gtgcgggagg
    15721 cgttcatcaa gttggtcgaa gcgggcgtgc tgcaggtgtt tccgcagcgt ggcacctttg
    15781 tgaagcgtat ttcgccgcgc caggtgcgcg aagggcgctt cattcgcgaa gcgatcgaga
    15841 cggcggtggt gcgcaaggca gccgtgtcga tcaccgacca gcaattgcag gcactcgccg
    15901 acaatcttcg cgaccagaag ctcgcggcga aatcgaacga caccgctgct ttcctcgcgc
    15961 tcgacgaggc gtttcattac gcgattgccc aggcgatcga ttgcacggcg gcatgggaaa
    16021 cgattcagga catcaaggcg cagatggatc gggtgcgcta tttgagtttg cctgacgtat
    16081 cgccgctcga tctgctgatc aagcagcacg caaaaattct cgcaggtctt cgcgcgcacg
    16141 acccgagcgc cgccgaagag gcgatgcgca gccatctgcg cgagattctg atgtcgctcg
    16201 gtccgattgc cgagcgcaat ccggcgtggt ttgaagcgga cgaaccggag cgcgtgccgc
    16261 tatcgacctg acgcggcgcg ctgctgcatt caaccggtat gctgcggtgc agcacgtcac
    16321 ttgcttttcg gctatagtcg ggttacggca tatgtccaga gaggtacaga atgtacgacg
    16381 ccgaaatcgc cgccaccctc ctgaatcgtt gggccacccg ttcctcggcc actgacttcg
    16441 ccacctatct cgagttgctg cgcgaaggca acctcagttt cacctatcaa tcgggacacg
    16501 tgcgcgaggt gggcattgaa gcgggtatcg aagcgggcat tgaagccggc gtcgaatccg
    16561 gtactgaggc gggaagcgcc atcaatatcg aatccctggt ctttggcgac ggctcgcgca
    16621 cgctgcgcgt cgaagcacct gacaggactc cccgctggac gcgctgggcg gcggtggaac
    16681 cgctgctgcc cgcacccagc gagacgtgaa cacaatgatc gtcagacgat ccgcacgccg
    16741 gcgcgatacg tcgcgcgcgg caccggcagt gtgtcgagca tcgtgacgcg cacgaagtcc
    16801 gcacgcaaac ccgtttcgat cgccccgcgg tcatgcaagc ccgccgtgcg ggccggctcg
    16861 gcggagacgg tcgccatggc gcgcggcagg gtccagccgg ctttactgac cagctcgaac
    16921 acggccgtca tcaaactgga cggcacgtaa tccgacgaca gaatatcgag caaatcggcc
    16981 ttcgccagtt cgagcgccga cacgttgccc gaatgcgagc cgccgcgcac gatattcggt
    17041 gcccccatga tggtcgaaat gccgtgctcg cgggccgcct cggcggcgat gcgggtggtc
    17101 gggaattcgg ccagcacgat gccctcggcc ttggcctgtt cgacgtgctc gatcagcgtg
    17161 tcgtcatggc tcgccaccgg aatgccgagc tgcttgcagc gcgcgacgat ctcgcggcgg
    17221 tgcgcatcgg cataacgttc ctgctcgacc gacagttcca ccagcgcggt tgcgacgtgt
    17281 tcgtcggtca atttgccgtt gcgttcctga aagcgccgcc attggtcgcg atcgtgccat
    17341 tgacgctggc cgggcgtgtg atccatcacc gatgcaagcc gcagcaacgg atgtgcgcac
    17401 aacgaatcga acatctcgac cacgtcggcg gtggcgatct cacagcgcag atgcagaaag
    17461 tgttcggcgc gcagcagctt gcgctcggag aagcgcgtga gggattcggc gcattgcgtt
    17521 tgctgatcgc gcccacgcag ccctactttc gagcgtgatc caatcgagag ggcgtcgaac
    17581 accgtggtga tgccggccgc cgccacttgc gcgtcgtgga tcacaaacgc ggcatcggta
    17641 ttccattgca cgcccggacg cggcaccaga tgcttctcga ggttgtccgt gtgcaattcg
    17701 atcagacccg gcaacagaaa gtcgccgtcc caatcttccg cttcgcgcgc cgaggtcgtg
    17761 ccacgctcga cgtcgcgaat cacgccgtct tcaatgcgta ccacgccggt aaatacttca
    17821 tctcgcgtca cgatgcgagc gttcctgatc aacatcgact tgctccgtag tctgttctgg
    17881 gcgttgcagc gccgtgcttc gacccagtat ttcagctcag tggttcagtt tagtgccgtt
    17941 tcgtgtcggt tagtgacgta acggcggctg cagttcgagg cggcgcgtgg ccaccttgtt
    18001 gcgggtatct tcgtcgtgaa agatgccgac gatcgccgcg ccacgctcgc gtgcttcgac
    18061 gatcagatcg gcgaccacgt cgcggttctc cgcgtcgagc gaggccgtgg gctcgtcgag
    18121 cagcagtagc ggatgcccgg cgatcaagcc gcgggcgatg ttcacgcgtt gctgttcgcc
    18181 gccggagaag gtggcgggcg cgagcgacca cagccgctca ggcacgttca atctcgccag
    18241 cagtgcggcg gcacgcgcgc gggcttcgtc ttccgccacg ccgcgcgaca gcagcggttc
    18301 ggcgacgagc gtaagcgtcg tcacgcgcgg aatcacgcgc agaaactggc tgacgtagcc
    18361 gaccacgccg cggcgcagac gcagcacgtc gtgcggttcg gcgccggtga tcgacaccgg
    18421 ctgcccatca tccgccgccg cgtcgcgaat cgcaatggag cctgtactgg ccagataatt
    18481 gccgtacaaa cagcgcagca gcgtactctt gccggcgcct gacggcccga ccagcacgac
    18541 acactcgccg cgctcgacat cgagcgagac gcccgcgagt gcttcgatct ggacgccgcc
    18601 ctggccgtgc aacgtgaaag tcttgccgat gccgaccgcg cgcagcatca gtgcattatt
    18661 ctcgatgaac gcgcgttcag cggcgtgtga ttccttgctt gtttcaatgg atcgcattgt
    18721 gagcctcaaa ccggcagaac cgaggaaacc agcgtttggg tgtaagggtg ctgcggatcg
    18781 tcgagcacct gatcggtcag gccggattca accacctggc cgccttgcat gaccatcaat
    18841 cgatgtgcca gcagacgcgc cacgccgata tcgtgcgtca cgatcagcac cgacagatgc
    18901 agtgtggacg tgagcgtgcg cagcagatcg agcaggcgcg cctgcaccga gacgtcgaga
    18961 ccggcggtcg gctcgtccat gaagacgagg cgcggaccgg tgacgagatt gcgcgcgatc
    19021 tgcaaacgtt gctgcatgcc gccggaaaac gccgccggaa attcgtcgat gcgagtggcg
    19081 tcgagttcga cgcgctccat ccactgcgtc gccgtgtggc ggatgtcgcc atagtggcgg
    19141 gcaccgacgg ccatcaacgg ttcgccgata ttcgcaccgg cggagacgcc gctgcgcagg
    19201 ccgtcgcgcg gattctgctg cacgaagccc cactcggtgc gcatcaacag acgccggcgc
    19261 ggttcggaca aggcgagcag atcgagcttt tcgccgtgcg tggccgtgta gtggagcgag
    19321 ccgctatcgg ccgcggattt cagggcgagc gtattcagca gcgtggtctt gcccgagccg
    19381 gattcgccga cgatgcacag cacttcgccc ggatacagat cgaagctgac gttcctgcag
    19441 ccgttgcggc cgccaaattg tttggtgagc gaacgggcgc tcaacagcgg cgtcatgcgg
    19501 tttctccttt gcgtgcttcg tatgacggca actcgaggtc ctgatcgccg cggcgttcat
    19561 ggcagtaatc gctatccgag cacacgaaca tgcgtttgcc ggcgtcgtcg acgatcattt
    19621 cgtcgaggaa gctttccgtc gatccgcaca gcgcgcacgc gtgctgccac ttctgtactt
    19681 cgaacggatg atcgtcgaag tcgagactgc gcacgggcgt gtatggcgga atggcgtgaa
    19741 tgcgtcgttc gcggccggcg ccgaacagtt gcagcgcggg gttcatgtgc atcttcggat
    19801 tgtcgaattt cggaatcggc gacggcgaag tcagatagcg atggttgacg atcaccgggt
    19861 agtcgtacgt ggtggcgatg ctgccgtgat gcacgatgtc ctcgtacagc ttcacactga
    19921 tcaacccgta gtcggccagt gcgtgcaact tcttgcattc ggtagcgcgc ggttccagct
    19981 tgtagagcgg ttcgggcatc ggcacttgat agaccagaat ctgcttctcg gtgagcggcg
    20041 cttcgggaat ccggtggcgg gtctggacga tcgtcgcttc agtcgtgcgg cgcgtggtcg
    20101 ctacgcccgt ggtccgcgcg aagaagcggc ggatattgac cgcgttagtg gtttcgtcgg
    20161 agccctgatc gatcaccttc agcgtatcgt tcttgccgat gatcgcggcc gtcacctgaa
    20221 tgccgcccgt gccccagcca tacggcaagg gcatttcgcg cgaggcgaac ggcacctgat
    20281 agcccggcac ggcgaccgct ttgagcaacg cgcggcgcag catgcgcttg gtctgctcgt
    20341 cgaggtaggc gaagttgtag ccgtccgcgg cgctgtcgta cgaagcggtg gcgagagata
    20401 tgtccggcgc gttcatgcgg cttgctcctg ctgatcctga tcctggcctt gatttttatc
    20461 cgctgcgccg tgctgtgccc gcaagcgccg cacgagttcg agttcggact ggaagtcgac
    20521 gtagtgaggc aatttcagat gctgaacgaa gccggacgct tcgacgttat cgctgtgcga
    20581 gagcatgaat tcgatgtcct gcgtcggcga ggcgagtgtt tcgccaagct cctcggcacg
    20641 caacgcgcga tccaccagcg ccatcgccat cgctttgcgt tccgagtggc cgaacgcgag
    20701 gccgtagcct tgcgtgaaag caggcggcac gctgccgctg ccggcgaact ggttgatcat
    20761 ctggcattcg gtgatgtcga tctcgccgat ctcaacggct tcgcccaatt cgtcgagttc
    20821 catttcgacg gcgatcgcgc caaagcggat ctcgccggca aacggatgcg tatgcgcgta
    20881 gccgcgttgg gttgcatagc ccatcgcaag caggaaacct tcgtcgccac gagccagatt
    20941 ctgcaatcgc gtggtgcggc tcgcgggaaa agcgagcggc tcgcgcgaga ggtcgccggg
    21001 ttccggcgcg ttcggcgtgg ggcgttcctg ctcgatcaga ccttccttgt cgagcagcgt
    21061 gaccacgcgc ggcatggctt ctgccgcggc ctgggcggtt tgaatggacg aggtcgttgc
    21121 ggccgcatcg attccgctat cgccatcgcc ttcggcgagc aaggcgaaat ccagcagacg
    21181 ctgcgtgtaa tcgtaagtcg cgccgagcaa ctggccgccg ggcacgtcct tgaaggtcgc
    21241 cgaaatgcgg cgctccactt gcatggcttc cgtgtcgatc gcctgcgtgt aaccgaaacg
    21301 cggcaacgtg gtgcgatacg cacgcaacag aaagatcgct tcgaccagat cgcccgctgc
    21361 ctgtttgatc gcgagcgcgg cgagttcttc gtcgtagacc gaaccttcgg tcatcactcg
    21421 cgcaacggcg agacgcaatt gctcgcgaat ctgcgcgacg ctgagttcgg ccagccgcgt
    21481 gtcgccgcgg cgtgccttgt cgagcagacg ccacgacgct tcgatcgcgc gttctccacc
    21541 cttgacggca acgtacatca gttcaccttc gcttgagttg tgcgcggcag gccgatcaac
    21601 gtattgccgc agacgagata acaatcgacg ccgcacggaa agagcggcgc gagggcggcg
    21661 cgttcacgcc agaaatgctc gggcaggccg accggtgcaa tcgtcgccgt gtgctggatg
    21721 cacgggccgc tcaggacgac cggcgcgcca cccgtcagcg cgtcgacgcg gatcat
//
LOCUS       DATASH010000595         1885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5835123,
            whole genome shotgun sequence.
ACCESSION   DATASH010000595 DATASH010000000
VERSION     DATASH010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5835123"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..96
                     /locus_tag="VHV99_16230"
     CDS             <1..96
                     /locus_tag="VHV99_16230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX3637003.1"
                     /translation="ALWSPSYFAGSCGGAPVSVIRQYIEQQRTPH"
     gene            complement(177..1284)
                     /locus_tag="VHV99_16235"
                     /pseudo
     CDS             complement(177..1284)
                     /locus_tag="VHV99_16235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426782.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
     gene            complement(1375..1641)
                     /locus_tag="VHV99_16240"
     CDS             complement(1375..1641)
                     /locus_tag="VHV99_16240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637004.1"
                     /translation="MNRNHALAASLVFSLAALNAVTVANAASEDDQAKACRGDAMHFC
                     SAEIPNKEKITACMKQHVDELSPACRAMFKGGKKGDAKAQAASQ"
BASE COUNT          339 a          611 c          619 g          316 t
ORIGIN      
        1 gcgctttggt cgccgtccta ttttgccggt agctgcggcg gggcgcctgt ctcagtgatt
       61 cgccaataca tcgagcaaca gcgaacgccg cactaaggcc ctggaaggac gcctacggcg
      121 tccgcgcttt ccttccccgc cgtgaacgac ggggcttgcc gcgcacttgg gtcaaattaa
      181 ccgccggacg cttcgctcgc gtgacgcgac gactggatca ccatcaacag atcgcccggc
      241 tcgatcggac acggctcgtc ttcgtagaac gacagcatct gtcccgcgcg tatcagcccg
      301 accaccaccg cgcccggcag cgcattgctc cagcggccca cttcgagcgc cgtcgccgga
      361 cgctccacca gattgacgcg gccgcgcgcc gacagcagat cgttgacgaa tggcacgatg
      421 tagttgctgt ccaccgcgtc ggcgagcaac aggccgccga tcttggtcga tgaaacgatc
      481 acgtccgcgc cggcctggcg caactggcgc tggtaggttt gctcctggat ccgcacgacg
      541 atcttcgtgt ccggcgcgac actgcgcacg ctcaacgtca gcaggatcgc gatggcgtcg
      601 tcgctgacgg cgatgatgac cgcgcgggca atgcgcacct gcgcctgttg cagcagatcc
      661 tcgcgtgatg gatcgccgag caggccggcc acgcccaagg ccgtggccgc ttcgagcgcc
      721 gcctgctgcg aatcgatgac gacgatcgac tccgcggcga agccgctttc cagcagctcg
      781 cgcaccgcga ccgaaccgct caatccgtag ccgcacacca cgacgtgatc gcgcagctgt
      841 ttttgcaggc gtttcatgcg gaattcctcg atgacacgtt gaatgacgaa ctgataagcg
      901 gtgcccagaa acacgaacca gatcacgatc cggatcggca cgataaagaa ggcgtcgatg
      961 agtcgcgcac gggcggtgac cggcacgatg tcgccataac cgacggtggc gacggtgacc
     1021 atcgtgaagt acatcaggtc gacgatgccg agcaccttgc ggttggcgtc gcgcagacca
     1081 tcgcggtcga gatacagcac ggcgaaggcg agcacgcaga ggccgagcac gagatagata
     1141 cgctgcagca gcatgcgctg cggcgacgcc gcggggcgcg tgaacagcgt gcgcgcgcgg
     1201 cgcctgccag ggtgtgcgag cccggcgcgg caagcgaaag cgtcgtgggc gtggctgcgg
     1261 cccggggtgt ggctgatcag tcacgcggcg gctccaggaa tcgggaacaa gcgattctac
     1321 gacgcctcgc gcgccgcggg gccggatcgg gagcaagcgg ccgcgccgcc gcctttactg
     1381 cgaagcggct tgggctttgg cgtcgccttt cttgccgccc ttgaacatcg cgcggcacgc
     1441 cggactgagt tcgtcgacgt gctgcttcat gcaggcggtg attttttcct tgttcggaat
     1501 ctcggcggag cagaaatgca ttgcgtcgcc gcgacaggcc ttggcctggt catcttctga
     1561 ggccgcgttc gccacggtca ctgcgttcag cgctgcaagg ctgaagacaa ggctggcggc
     1621 aagggcgtga tttcgattca tgacgggttc cgctatcgca aaggtcggtg acacgttgct
     1681 cagcagaaaa cgggcccgtt cattgcaccc gccatacggc gtgccggccg ccgcggctag
     1741 cgcgcgatgc gggtgagggt catgccacgc cctgcgtaga aacgcgccat cgttgcgagc
     1801 ggcaaataca cgttcgcctg gtgaggcgct atgcccgacg ggttatgaaa ccagacgccg
     1861 tcgcgatcgc gcccgtgcag cagga
//
LOCUS       DATASH010000596         2228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5835208,
            whole genome shotgun sequence.
ACCESSION   DATASH010000596 DATASH010000000
VERSION     DATASH010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5835208"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VHV99_16245"
     CDS             <1..318
                     /locus_tag="VHV99_16245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX3637005.1"
                     /translation="AASPTRTDIAAPNLAAIGLRLVDQTVLSLGPFQRASEFIYLNGD
                     NQPVVVISTLALLAPSQPQWAARRICEIRLLTWTAQRQRFVVAGDARTHGLMRAADVM
                     TMR"
     gene            462..980
                     /locus_tag="VHV99_16250"
     CDS             462..980
                     /locus_tag="VHV99_16250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3637006.1"
                     /translation="MDVRDELMEHVPRLRRYARALINNRDLADDLVQDTLERALGRSG
                     LFQPGTDLRAWLFTIMHNVFANQARKASARAVHVAVDDDSVHESEFAVPSDQVRSLEM
                     RDLDYALQRLPMEQREVVLLVGLEEMSYADVALALNIPIGTVMSRLSRGRERLRALMA
                     GTQPGAKLQVVR"
     gene            977..1804
                     /locus_tag="VHV99_16255"
     CDS             977..1804
                     /locus_tag="VHV99_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013343255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX3637007.1"
                     /translation="MSDQHTPIGEEDLHAYVDGTLSDARRVEVERALEQNPELATRVS
                     DYFSLNNMFHERYDRVLSEPVPKRLQTHWAGTRRWRMAANWPQFAGMAAALVLGVGIG
                     VGTHMGQDVVAPVAGHSDTRPVSADSSEMFARQAAVAHVVYMPAIDRPAEMTADHEQD
                     FVQWLSNRLGTNVHPPMLSKSGFQLSGGRLLPGADGPTAQFMYRGPNGERVTLCISHR
                     QVNSNTTAFKLYQDGPVNVFYWVDGDFGYAVSGGIDRKVLLQLSHNVYSQLTGAAPG"
     gene            complement(1838..1954)
                     /locus_tag="VHV99_16260"
                     /pseudo
     CDS             complement(1838..1954)
                     /locus_tag="VHV99_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006493459.1"
                     /note="acts as a molecular chaperone to fold and export
                     lipase A; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipase chaperone"
     gene            complement(2133..>2228)
                     /locus_tag="VHV99_16265"
     CDS             complement(2133..>2228)
                     /locus_tag="VHV99_16265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX3637008.1"
                     /translation="ALWSPSYFAGSCGGAPVSVIRQYIEQQRTPH"
BASE COUNT          393 a          697 c          735 g          403 t
ORIGIN      
        1 gctgcctcgc cgacgcgaac cgacatagcc gcgcccaacc tcgcggcgat cgggctgcgg
       61 ctcgtcgatc agacggtcct atcgctcggc ccgttccaac gtgcgagcga gttcatctat
      121 ctgaacggtg acaatcagcc agtcgtagtg atttccactc tggcactgct cgcgccgtcg
      181 cagccgcagt gggccgcgcg ccgcatctgc gagattcgcc tgctcacgtg gacggcgcag
      241 cggcagcgct tcgtggtcgc cggcgacgcc cgcacccacg gtctgatgcg tgccgccgac
      301 gtcatgacca tgcggtaagc cttttcccgg ttttcgcaac acgccaaaaa acaacggaat
      361 aaaatgcgcc cacgcgtgtt cgcatgttga atgcgcgcgg tggttcaatc gacggccgcc
      421 cccgcggcgc ctcacgagaa gccccacgat tgggatacaa catggatgtc cgtgacgagt
      481 tgatggagca tgtgccgcgc ttgcggcgct atgcgcgagc gttgatcaac aatcgcgatc
      541 tcgccgacga tctggtgcag gacacgctcg aacgcgcgct gggccgcagc ggcctgttcc
      601 agcccggcac cgacctgcgt gcgtggctgt tcacgatcat gcataacgtg ttcgccaatc
      661 aggcgcgcaa ggcttcggcg cgtgccgtcc acgtggcggt cgacgacgac agcgtccacg
      721 aaagcgaatt tgcggtgccg tccgaccagg tccgttcgct ggaaatgcgc gacctcgact
      781 acgcgttgca acgcctgccc atggagcagc gcgaagtcgt gctgctggtc ggcctcgagg
      841 aaatgagtta cgccgacgtc gccctcgcac tgaacattcc gatcggcacg gtgatgtcgc
      901 gcctttcgcg cgggcgcgag cggcttcggg cattgatggc gggcacgcag cccggtgcaa
      961 aattacaggt ggtgcgatga gcgaccaaca cacgccgatc ggcgaagaag atctgcacgc
     1021 gtacgtggac ggcacgctgt ccgacgcacg acgcgtcgag gtggagcgtg cgctcgaaca
     1081 gaaccccgag ttggccaccc gtgtcagcga ttatttctcg ctgaacaaca tgttccatga
     1141 acgctatgac cgcgtgctga gcgagcccgt gcccaaacgt ctgcagacgc attgggccgg
     1201 tacgcgccgc tggcgcatgg ccgccaactg gccgcagttc gccgggatgg ctgcggcgct
     1261 ggtgctcggc gtgggcatcg gcgtgggcac gcatatgggg caggacgtcg tggcgccggt
     1321 cgccggtcac tcggacacgc gcccggtcag tgcggacagt tcggaaatgt ttgcgcggca
     1381 ggccgcggtg gcgcatgtgg tctatatgcc cgccatcgac cgtccggctg aaatgaccgc
     1441 ggatcacgaa caggatttcg tgcaatggtt gtccaaccgg ctcggcacca acgtgcatcc
     1501 gccgatgctc tcgaagagcg gcttccagct ctcgggcggg cgcctgttgc caggcgccga
     1561 cggaccgacc gcgcagttca tgtatcgcgg accgaacggc gaacgtgtga cgctgtgcat
     1621 ttcgcatcgt caggtgaact cgaataccac cgcgttcaag ctgtatcagg acgggccggt
     1681 gaacgtgttc tactgggtcg acggcgactt tggctatgcg gtgtcgggtg ggatcgatcg
     1741 caaggtgctg ttgcaactgt cgcataacgt gtattcgcag ttgacgggtg cggcgccagg
     1801 ttgataggcc gcgccgagcc gcaccgcgat cgatgagtca gtttgcagaa ccgctgccgc
     1861 gatcgaacga cgcggcgcgc atcgcgtcgc ccggattggt gaagagccgc tggcgcaact
     1921 gcgcgatctg cgcgtcgcgt tgccgaggcg gcaagctatg acgcagtgct tttttcaggt
     1981 ttatcgccgt gtcgcactgc ggcgcgcatt gaacgtatcg agttcgataa tgctgcctgg
     2041 gtccgcgccg gacttgaccc aagtgcgcgg caagccccgt cgttcatggc ggggaaggaa
     2101 agcgcggacg ccgtaggcgt ccttccaggg ccttagtgcg gcgttcgctg ttgctcgatg
     2161 tattggcgaa tcactgagac aggcgccccg ccgcagctac cggcaaaata ggacggcgac
     2221 caaagcgc
//
LOCUS       DATASH010000597         5365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5835231,
            whole genome shotgun sequence.
ACCESSION   DATASH010000597 DATASH010000000
VERSION     DATASH010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5365
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5835231"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(493..741)
                     /locus_tag="VHV99_16270"
     CDS             complement(493..741)
                     /locus_tag="VHV99_16270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide MXKDX repeat protein"
                     /protein_id="HEX3637009.1"
                     /translation="MKKFAIAAMALALLTSGGAAFAQASGAMSNDSMSKDAMSKDTMS
                     KDSMSKDSMKKGTMKHDQMKKGSDSMGMKHEASGAMAN"
     gene            complement(863..1624)
                     /locus_tag="VHV99_16275"
     CDS             complement(863..1624)
                     /locus_tag="VHV99_16275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012404274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX3637010.1"
                     /translation="MPDDKLDRESILIDARRELAMPSRRLFGKRLLTLGGLSMLTGCS
                     LTDNDSVNAFLTAVSRLNDRAQAALFDPKQLAPTYTEAQITRPFPFNAFYSIDDVPDV
                     DASSYTLKISGLVTGQRVWTLPELYALPHAEQITRHICVEGWSAIGRWGGTPFSDFLR
                     RIGADTSAKYVGFKCADDYYESIDMPTALHPQTLLTFSYDGERLPAKYGFPMKLRMPT
                     KLGYKNPKHIVEMFVTNTYPGGYWVDQGYNWFGGS"
     gene            complement(1717..2355)
                     /locus_tag="VHV99_16280"
     CDS             complement(1717..2355)
                     /locus_tag="VHV99_16280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006052446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEX3637011.1"
                     /translation="MATPHALNDAAPPAIPKRNTIHPVWVRVTHWLNALAAIVMMLSG
                     WRIYDASPIFKGFTFAPAITLGGWLGGALQWHFAAMWLLVFNGLLYLALNLATGRFVR
                     KFLPVSPRAVLHDFIAALTGKLSHADLRVYNAVQKFAYLFVVCDLLVLVLSGLAIWKS
                     VQFPLLRELMGGYDNARIVHFCAMSLMAAFIVVHLAMVALVPRSLLAMLRGH"
     gene            complement(2401..3231)
                     /locus_tag="VHV99_16285"
     CDS             complement(2401..3231)
                     /locus_tag="VHV99_16285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HEX3637012.1"
                     /translation="MQTAQTRRANRYMTRAIALAVALTALSASGLATAAGAVCASHSP
                     SHRVALVELYSSEGCNSCPPADNWLSQWKDSGATQGIVPLALHVDYWNSLGWTDRFSQ
                     HRFTERQQALTDLAGGHIIYTPEVFVSGRELRNWSQRDSFQNRIGNVVAEPALANVAL
                     ELKPQAKGAFNVDAQFTSKATDTTGSLNAYVAVYQNALNSQVRAGENSGASLHHERVV
                     RQWIGPVPLVAGNAQIHRDIRIDDADANAPADHFGVVAFVENAATGDVLQVGELAACN
                     "
     gene            complement(3423..3584)
                     /locus_tag="VHV99_16290"
     CDS             complement(3423..3584)
                     /locus_tag="VHV99_16290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637013.1"
                     /translation="MTGSRKVAPGHQGRRGAAVNAPVGRVGRDGGVLSLAPKNAAQDT
                     RVTVVVSYD"
     gene            3996..4406
                     /locus_tag="VHV99_16295"
     CDS             3996..4406
                     /locus_tag="VHV99_16295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HEX3637014.1"
                     /translation="MKKAKTCYLTELDVARLEKHAAAPGADAKLQDMLDDVLERAVIV
                     ESRDIPANIITMNSQATLVDEASGEQMTWTVVYPPDAEFAQGRLNVFSPLGLALLGAR
                     RGERIRFTPPSGTEKVLKLEKILFQPEAADDFTL"
     regulatory      4561..4679
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4858..>5365
                     /locus_tag="VHV99_16300"
     CDS             4858..>5365
                     /locus_tag="VHV99_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065424.1"
                     /note="catalyzes the formation of
                     4-amino-2-methyl-5-phosphomethylpyrimidine from
                     5-amino-1-(5-phospho-D-ribosyl)imidazole and
                     S-adenosyl-L-methionine in thiamine biosynthesis; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEX3637015.1"
                     /translation="MNANPKFLSADAHVDAAAVAPLPNSRKVYVTGSRADIRVPMREI
                     SQADTPDSFGGEKNPPVYVYDTSGIYTDPDARIDIRAGLPALRHSWIEERGDTEALTG
                     LSSDFSRERAADTATAELRFKGLHRTPRRAIAGKNVSQMHYARKGIVTPEMEYVAIRE
                     NQRRAEYLE"
BASE COUNT         1002 a         1676 c         1661 g         1026 t
ORIGIN      
        1 gttgcggcga catgggttca atccgcctgt gctgctcgag gtgctgcgcc cccgctaaaa
       61 gccctcattc gggatggcgg acaccgcatc ggcgatgcgg tctggcgggc gaggctggcg
      121 gacatgtcgg tggaacagaa ggacgataga tggccaatgg ggccacaacg ccgcggcagt
      181 cgaaagaaat cgattcacgg ctgacacgcg ccaaacgttt ggcgcgacgc cgcattatgc
      241 agtaagcgcc caccgcgacg ctcgaaagaa tcgtaacttt tcggcgtact tgcaacaaaa
      301 aagccgggag tgttcccggc ttggactgct gaccaacccc acgttttgag gagcgcgaag
      361 gtttgttttt caggcgcggc aaatttgcgc ggagcgctag tttgaggcaa gcagagccgg
      421 gggcgtcccc cgacttttgc accgcccgta ttaggggcgc tcaagcgatg caagcggcat
      481 cggcggtaac gctcaattgg ccatcgcgcc cgaggcttcg tgcttcatgc ccatcgagtc
      541 gctgcccttt ttcatctggt cgtgcttcat tgtgcccttc ttcatgctgt cctttgacat
      601 gctgtccttc gacatcgtgt ccttggacat tgcatccttc gacatcgaat cgttggacat
      661 cgcgccgctt gcctgcgcga atgcggcgcc gccgctggtc agcaaagcga gggccattgc
      721 tgcgattgcg aactttttca tgggataact ccgtttcgtt atggatgaat cagaaccggc
      781 tttcaactcg agccgggtat ttccgaagcc tctgccgccc ggaggtcgcg cgtgcagcct
      841 ggcacgcggc gacccgcgcg cgtcaggagc ctccgaacca gttgtaaccc tggtcgaccc
      901 aatagccgcc gggatacgtg ttagtgacga acatctcgac gatgtgcttc ggattcttgt
      961 agccgagctt ggtcggcatg cgcagcttca tcgggaagcc gtatttcgcg ggcagacgct
     1021 cgccgtcgta ggagaacgtg agcagcgttt gcggatgcag tgcggtgggc atgtcgatgc
     1081 tttcgtaata gtcgtcggcg catttgaagc cgacgtattt cgcgctcgtg tccgcgccga
     1141 tgcgccgcag gaaatcgctg aacggcgtgc cgccccagcg gccgatcgcg ctccaccctt
     1201 ccacgcagat atgccgcgtg atctgctcgg catgcggcag cgcgtacaac tcgggcaggg
     1261 tccagacacg ctgaccggtg acgaggccgc tgatcttcag cgtgtagctg gaagcatcga
     1321 catcggggac gtcgtcgatg ctgtagaacg cattgaacgg aaacggccgc gtgatctgcg
     1381 cttcggtgta ggtcggcgcg agttgcttcg gatcgaacag agcggcctgc gcgcggtcgt
     1441 tcagacgcga cactgccgtc aggaacgcgt tgacggaatc gttgtcggtg agcgagcagc
     1501 cggtcagcat cgagaggccg ccgagcgtta gaagccgttt gccgaacagg cgccgcgacg
     1561 gcatggcgag ttcgcgccgg gcgtcgatca ggatcgattc gcggtcgagc ttatcgtcag
     1621 gcatagggcc ggcctttcga tcggtttttg tccagggctt attcatgatt cgatatcctt
     1681 aagcttcgcg tagccgtctg tcggtgcatg agcggcttag tgtccgcgca gcatcgccag
     1741 cagcgagcgc ggcacgagcg ccaccatcgc cagatgcacg acgatgaacg ccgccatcag
     1801 cgacatcgcg cagaagtgga caatgcgcgc gttgtcataa ccgcccatca attcgcgcag
     1861 cagcggaaac tgcaccgatt tccagatggc aagacccgag agcaccagca cgagcagatc
     1921 gcagaccacg aatagataag cgaatttctg caccgcgttg tagacgcgca gatcggcgtg
     1981 tgagagcttg ccggtcagtg cggcgatgaa atcgtgcagc acggcgcgcg gcgagaccgg
     2041 caggaacttg cgcacgaagc gtccggtggc gaggttcagc gcgagataca acagaccgtt
     2101 gaagaccagc agccacatcg ccgcgaaatg ccattgcagc gcgccaccga gccaaccgcc
     2161 gagcgtgatt gccggcgcaa aggtaaagcc cttgaagatc ggcgacgcgt catagatgcg
     2221 ccagccggac agcatcatca cgatcgccgc gagtgcgttg agccaatgcg tgacgcgcac
     2281 ccacacaggg tgaatcgtgt tgcgcttcgg tatcgcgggg ggagcggcat cgttgagagc
     2341 gtgaggcgtt gccatgagaa tctccgatga aatgggaggc gctgtggagt cgtcgcgcga
     2401 tcaattgcag gcggccagct cgcccacttg caacacgtcg ccggtcgcgg cgttttccac
     2461 gaaggcgacg acgccgaagt ggtcagccgg cgcgttggcg tcggcatcgt cgatgcggat
     2521 atcgcggtgg atttgcgcgt taccggcaac gagcggcacg gggccgatcc attgccgtac
     2581 gacgcgttcg tgatgcaagg atgcgccgct gttttcgccg gccctgacct gcgagttcaa
     2641 cgcgttctgg tacaccgcga cgtaagcgtt cagtgagccc gttgtgtccg ttgccttgct
     2701 ggtgaattgc gcgtcgacat tgaaggcgcc cttcgcctgg ggcttcagtt ccagcgcgac
     2761 gttcgccagc gccggttcgg cgacgacgtt gccgatgcgg ttctggaaac tgtcgcgttg
     2821 cgaccagttg cgcagttcgc ggcccgatac aaagacttcc ggcgtgtaga tgatgtggcc
     2881 gccggcgaga tcagtgaggg cttgctgacg ctcggtaaag cgatgctgcg agaagcggtc
     2941 ggtccagccg aggctattcc agtagtccac gtgcagggcc aacggcacga tgccttgagt
     3001 ggcgccgctg tctttccact ggctcagcca attgtcggcg ggcggacagc tgttgcagcc
     3061 ttcgctgctg tagagctcca ctagcgctac gcggtgggat gggctgtgcg atgcgcatac
     3121 ggcgccggct gcagttgcaa ggccgctggc cgagagggcc gtcagcgcta cggccagcgc
     3181 gattgcgcgg gtcatatagc gattggctcg gcgggtttgt gcagtttgca tggcgtgctc
     3241 catcagaatc tgcacgtttg tcgaaccacg agaccggtta tgacaacccg ttttgaattt
     3301 ttattgttgg tttgagttgt tcgcccacgg tcgcgaccat gccttgctgt ttatgccgac
     3361 ggggcgcggc atgtctgtac gttggcgggc aaactgccgc gaaacgcccc gctgtggccg
     3421 cctcaatcgt aactgacgac caccgtcacc cgggtatcct gcgcggcgtt cttcggcgca
     3481 agcgacaaca ccccgccgtc gcggccgacc cgccccactg gcgcattaac ggcagcgccg
     3541 cggcggcctt gatggcccgg tgcgacctta cgcgatccag tcacgaccct gcgccgcccg
     3601 gtgcgatagg cgcataccgc accccgtcca ggcagtacct cccggccgca attaaggaga
     3661 cgcccggccc gtgctaacat tcacctttcg attgcgccga tcaagctttc tttccggcgc
     3721 tcacccaaac aactttcact ctctgccggc tacctcgatg cgctttccga tctcccgtgc
     3781 cttcaaccgc ttgcgccccc gccgcgcaac cggttgcgcg cgcgcggacg tctcgacgcc
     3841 gcaccccagc cggccgtttc gaacgctccc gcttctcgcc gcccggcgcc cgatctgacg
     3901 ttgcttcctc cattaccgga gccacgtcgt cgggctccgg gctgatctca tccgtacgca
     3961 attcccttat atgcagatca attggagaac ccgtcatgaa aaaggcgaaa acctgttacc
     4021 tcaccgaact cgacgtcgcc cggcttgaaa agcacgcggc cgcacccggt gccgacgcca
     4081 aattgcagga catgctcgac gacgtcctgg aacgcgccgt gatcgtcgaa tcgcgcgaca
     4141 tccccgcgaa catcatcacg atgaattcac aagccacgct ggtcgacgaa gccagcggcg
     4201 agcaaatgac ctggacagtt gtctatccgc ccgatgcgga gttcgcgcag ggccgcctga
     4261 acgtgttttc gccgctcggt ctcgcactgc tcggcgccag gcgaggcgaa cgcattcgct
     4321 tcacgccgcc aagcggcacg gagaaagtgc tgaagctcga aaagatcctg tttcagcccg
     4381 aagccgccga cgatttcacg ctgtaacgca aaacacgctc gagattcaaa tttccgcacc
     4441 gcatcacacc cggcagccct atcaccataa gaatagtgcg tggctgaccg gcgttatctc
     4501 cgacatgtga ctttttcaag acgggttgcc aagcagcgcc cgggcggtgt atcgtgtgcg
     4561 ctgctaacgc gggggtcctg cgtggcgcgg gggttggagg tccgtgctgc gtgggtgaga
     4621 aatacccttt gaacctgatc tggataatgc cagcgcaggg aagcgtacgg atttcgcacc
     4681 ccagctttac cgccttacct ctcgcgaatc cgacaacttc catctcgtct cctgcttagc
     4741 ggccccacac atgggaccgg agttggtgct ttgcgccaac tctcgtcgac cgcaacaagt
     4801 tgcatgggac cggagtaagc gctaaagcgc caacgccggt cgacagagga gatccgcatg
     4861 aacgccaatc cgaagtttct ttccgccgac gcccacgtcg acgccgccgc tgtcgcgccg
     4921 ctgccgaatt cccgcaaggt ctacgtgact ggctcgcgcg ccgatatccg cgtgccgatg
     4981 cgcgagatct cgcaggccga cacacccgac agcttcggtg gcgaaaagaa tccgccggtc
     5041 tatgtctacg atacgtcggg tatttacacg gatccggacg cgcgcatcga tattcgtgcc
     5101 ggcctgcccg cgctgcgcca cagctggatc gaggagcgcg gcgataccga agcgctgacc
     5161 ggtctgtcga gcgacttcag ccgcgagcgc gccgccgaca cggccaccgc cgagttgcgc
     5221 ttcaagggcc tgcaccgcac gccgcgccgt gccatcgccg gcaagaacgt ctcgcagatg
     5281 cactacgcgc gtaaaggcat cgtcacgccg gaaatggagt acgtcgcgat tcgcgagaac
     5341 cagcgccgcg ccgagtatct ggaga
//
LOCUS       DATASH010000598         1764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5440483,
            whole genome shotgun sequence.
ACCESSION   DATASH010000598 DATASH010000000
VERSION     DATASH010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5440483"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1341
                     /locus_tag="VHV99_16305"
     CDS             <1..1341
                     /locus_tag="VHV99_16305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX3637016.1"
                     /translation="VFVPIDPRAKGEKLAFMLDNARCRGVIAADYALDNLFAVRASLP
                     QLSWVIGLETDEGAKPLADFAGVLPYRALLPDRVPELPIVARDPDSAMQLIFTSGTTG
                     DPKGIVMTHRRYCATSAAVSALFGYLPDDRPYAGLSLTHANAQIVTLGASLTAGMRAV
                     LSRRFTKSRLWDITRTYRCTTFTLLGGMTTAVYSQPSQPGDADNPVRFVISAGMPAAI
                     WKAFEQRFGVQIVEFYGAAEGGITIKPLGIGPIGSIGKPAPILSHRIVDEDGNDCAPG
                     VPGELLFRPADGSPFKVEYFGNPDASAKKCRDGWLYMGDVVRADENGWLFFEYRKGGG
                     IRHNGEFINPAPIEKVIAESGLVEDVYVYGVKAASGAPGEKDVVAAVVPKDAARFDPQ
                     QIFRACRAKLEASSVISSLQVVPEIPKTASEKPLERYLIAMFDEQRDSVFTETP"
     gene            1385..>1764
                     /locus_tag="VHV99_16310"
     CDS             1385..>1764
                     /locus_tag="VHV99_16310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEX3637017.1"
                     /translation="MKVLVPVKRVVDANVRVRVKADGSAVDIANVKLSMNPFDEISVE
                     EAVRLKEAGTASEVIAVSCGVPACQETLRTAMATGADRGILVDTDAELQPLAVAKLLK
                     ALVDKEQVQLVLLGKQAIDDDSNQV"
BASE COUNT          318 a          551 c          595 g          300 t
ORIGIN      
        1 gtgttcgtgc cgatcgatcc gcgcgcgaag ggcgagaagc tcgccttcat gctcgacaac
       61 gcgcgctgcc gcggcgtcat cgccgcggac tatgcgctcg acaacctctt tgccgttcgc
      121 gcatcgctcc cacaactgtc ctgggtgatc gggctcgaaa cggacgaggg cgcgaagccg
      181 ctcgccgatt ttgccggcgt gttgccatac cgcgcgttgc tgccggatcg ggtgcctgag
      241 ctgccgatcg tggcccgcga tcccgatagc gctatgcagt tgatcttcac gtccggtacc
      301 accggcgacc cgaagggcat tgtcatgacg caccgccgct attgcgcgac ctcggcggcg
      361 gtgtcggcgc tgtttggcta cctgcccgac gaccggccgt atgcgggcct gtcgctgacg
      421 catgccaacg cgcagatcgt gacgctgggc gcatcgctca ctgccggcat gcgcgccgtg
      481 ctgtcgcgcc gctttacgaa gtcgcgtctg tgggacatca cccgcacgta ccgctgtacg
      541 acgttcacgc tgctcggcgg gatgaccacg gcggtctaca gccagccgag ccagcccggc
      601 gacgcggaca accccgtgcg cttcgtgatc tccgcgggca tgccggcggc gatctggaag
      661 gcgttcgagc agcgcttcgg cgtgcagatc gtcgagttct acggcgcggc ggaaggcggg
      721 atcacgatca agccgctcgg gatcggcccg atcggcagta tcggcaagcc cgcaccgatt
      781 ctctcgcacc gcatcgtcga cgaagacggc aacgactgcg cgcccggcgt gcccggcgaa
      841 ctgctgttcc ggccagcgga tggctcgccg ttcaaggtcg aatacttcgg caatccggac
      901 gcgtcggcga agaagtgcag ggacggctgg ctgtacatgg gcgacgtcgt gcgcgcggac
      961 gagaacggct ggctgttctt cgaatatcgc aaaggtggcg gcattcgcca caacggcgag
     1021 ttcatcaatc cggcgccgat cgagaaagtg atcgccgaat cgggtctggt cgaggacgtc
     1081 tacgtctacg gcgtgaaggc cgcctcaggc gcgcccggcg agaaggacgt ggtcgcggcg
     1141 gtggtgccaa aggacgcggc ccgtttcgac ccgcagcaga tattccgcgc gtgccgcgcg
     1201 aagctcgaag cgagttcggt gatctcctcg ctgcaggtgg tgccggagat accgaagaca
     1261 gcgtcggaaa agccgcttga gcgctacctg atagccatgt tcgacgaaca gcgtgacagc
     1321 gtattcacgg aaacgccgtg acgcggcgcg ctcagccata cgatttttca gaacggagta
     1381 ggcaatgaaa gtacttgttc ctgtcaaacg cgtcgtcgac gcgaatgtga gagtccgcgt
     1441 gaaggccgac ggcagcgctg tcgacatcgc caacgtgaag ctctcgatga acccattcga
     1501 cgaaatcagt gttgaagaag ccgtgcgcct gaaggaagcc ggcacggcga gcgaggtgat
     1561 cgcggtttcg tgtggcgtac cggcctgcca ggaaacgctg cgcaccgcga tggcgacggg
     1621 tgccgaccgc ggcattctcg tcgacacgga cgcggagttg cagccgctcg cggtcgcgaa
     1681 gctgctgaag gcgctcgtgg ataaggagca agtgcaactg gtgttgctcg gcaagcaggc
     1741 catcgacgac gactcgaacc aggt
//
LOCUS       DATASH010000599         4806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_98610,
            whole genome shotgun sequence.
ACCESSION   DATASH010000599 DATASH010000000
VERSION     DATASH010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98610"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /locus_tag="VHV99_16315"
     CDS             complement(<1..1059)
                     /locus_tag="VHV99_16315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dehydroadipyl-CoA semialdehyde
                     dehydrogenase"
                     /protein_id="HEX3637018.1"
                     /translation="MTELLKNYVAGQWVAGGGEGVTLTDPVTGEALVRVSSQGLDLAH
                     AFGFAREEAGAALRERTYAQRAACLAEIVKLLQARRDDYYAIATANSGTTRNDSAVDI
                     DGGIFTLSYYAKLGASLGDVHALRDGNAVSLSKDPSFSVQHVLTPTHGVALFINAFNF
                     PSWGLWEKAAPALLSGVPVIIKPATATAWLTQRMVADVVDAGILPPGALSVVCGSSAG
                     LLDQIQAFDVVSFTGSAETAATLRAHPAFIEHGARLNVEADSLNSAILCADAAPGTPA
                     FDLFIKEVVREMTVKSGQKCTAIRRAFVPEASLEAVLDALEAKLAKITVGNPRNDTVR
                     MGSLVSREQYENVLAGIAA"
     gene            complement(1068..1547)
                     /locus_tag="VHV99_16320"
     CDS             complement(1068..1547)
                     /locus_tag="VHV99_16320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012400936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4863 family protein"
                     /protein_id="HEX3637019.1"
                     /translation="MSPPDFQRLIAGVTEQLAGRPLDAALGTWLNDTYPADGATFRAL
                     TEACRTGVAEGWLCNREGGGIRYGRIFKALPDTHGFSVDVVDMKDIAGPHHVHPNGEI
                     DLIMPLTAGATFDGHPAGWCVYGPGSAHRPTVANGQALVLYLLPQGAIEFTAAPSSV"
     gene            1733..2773
                     /locus_tag="VHV99_16325"
     CDS             1733..2773
                     /locus_tag="VHV99_16325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX3637020.1"
                     /translation="MNQNYSPAPVDDPADEDASRANRTDRGTGGGTDGGTDGGPERGA
                     ERGAEREERDPFLTAMGDRVRLLRARRGMTRKTLATETGLSERHLANLESGVGNASVL
                     VLRQIAATLNCPLAEVIGDETTASAEWLLIRELLQGRDQAALQRARVALAEMFAQAPR
                     DPHRKDRIALIGLRGAGKSTLGRMLAQERKVPFVELTRVIEQLAGCPPSEIHSLYGAS
                     AYRRYEHRALEAVIQEHERAVIASPGGLVSESGTFNTLLSHCYTVWLQATPEEHMRRV
                     VAQGDLRPMSGNKEAMDDLKRILVGRSELYGRADMTFDTSEKTLADAYLQLRDRLAAR
                     LAAESPDDARIG"
     gene            2891..4561
                     /gene="boxC"
                     /locus_tag="VHV99_16330"
     CDS             2891..4561
                     /gene="boxC"
                     /locus_tag="VHV99_16330"
                     /EC_number="4.1.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-epoxybenzoyl-CoA dihydrolase"
                     /protein_id="HEX3637021.1"
                     /translation="MSAAETAIAPVDYRTDPSQYKHWKLSFNGPVATLGIDIAEDGGI
                     RDGYKLKLNSYDLGVDIELHDAIQRIRFEHPEVRTVVLTSLKDRVFCSGANIFMLGLS
                     THAWKVNFCKFTNETRNGLEDSSRHSGLKFLAAVNGACAGGGYELALACDEIYLVDDR
                     SSSVSLPEVPLLGVLPGTGGLTRVTDKRKVRHDRADIFCTVVEGVRGERAKAWRLVDE
                     VVKPNQFDQAIQARALELAAQSDRPADAQGVVLTRIERTNREDGLTYKTLDITIDRAK
                     RIATFTAKAPQAGQPTEIDAIVAAGTSWWPLQFARELDDAILSMRTNELDVGTWVFRT
                     EGDARNLLAADATLMQHKDHWFVRETIGLLRRTLARIDVSSRSLFALIEPGSCFAGTF
                     AELAFACDRSYMAALPSNEDEEPAITLSEVNFGLYPMVTHQSRLARRFYEEAEPLDAV
                     RSKIGQAIKPVEAERLGLVTASPDDIDWADEIRIALEERAAMSPDALTGLEANLRFNG
                     PETMETRIFGRLTAWQNWIFNRPNAVGEKGALKVYGKGSKAQFDVSRV"
     gene            4654..>4806
                     /locus_tag="VHV99_16335"
     CDS             4654..>4806
                     /locus_tag="VHV99_16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HEX3637022.1"
                     /translation="MSTINYSDKIPNNVNLADDRALQRALEQWQPNFLSWWGDMGPEG
                     SHGYDVY"
BASE COUNT          931 a         1484 c         1536 g          855 t
ORIGIN      
        1 cgcggcaatg ccggccagca cgttctcgta ttgttcgcgg ctcacgagcg aacccatccg
       61 cacggtgtcg ttgcgtggat tgccgacggt aatcttggcg agctttgcct cgagcgcgtc
      121 gagcacggct tcgagcgatg cttccggcac gaacgcgcgc cggatcgcgg tgcacttctg
      181 accggacttc acggtcattt cccgcacgac ctctttgatg aacaggtcga aggccggtgt
      241 gccgggtgcc gcgtcggcac agaggatggc gctgttgaga ctgtcggcct ctacattcag
      301 acgcgcgccg tgctcaatga atgccggatg cgcacgcaat gttgcggcgg tttccgcgga
      361 gccggtgaac gacaccacgt cgaatgcctg aatctgatcg agcaggcctg cggaactgcc
      421 gcagacgacc gacaacgcac cgggcggcag aatgccggcg tcgaccacat cggccaccat
      481 gcgttgcgtg agccacgctg ttgccgtggc gggcttgatg atgacgggca cgcccgataa
      541 caatgcaggc gctgcttttt cccacaagcc ccatgacgga aaattgaacg cgttgatgaa
      601 gagcgcgacg ccgtgtgtcg gcgtcagcac gtgctgcacg ctaaacgatg ggtccttgct
      661 cagcgatacg gcgttgccgt cgcgcagcgc atgcacgtcg ccgagcgacg caccgagttt
      721 cgcgtagtac gacagcgtga agatgccacc gtcgatgtcg accgccgaat cgttgcgcgt
      781 cgtgcccgag ttcgccgtag cgatcgcgta gtagtcgtcg cgcctggcct gaagcagctt
      841 gacgatctcg gcgagacagg cggcgcgctg cgcgtaggtc cgttcacgca aggcggcgcc
      901 agcttcttcg cgagcgaagc caaatgcgtg ggcgagatcc agaccctggc tcgacacgcg
      961 caccagcgct tcaccggtga ccggatcggt cagcgtcacg ccttcgccgc cgcccgcgac
     1021 ccactggccg gcaacgtagt ttttcaatag ttcagtcatg gcgagcctca gacagaagat
     1081 ggagcggcgg tgaattcgat cgcgccttgc ggcagcaggt acagcaccag cgcctgcccg
     1141 ttcgcgacgg tgggacggtg cgcgctacca gggccgtaca cgcaccagcc ggccggatga
     1201 ccgtcgaagg tggcgccagc ggtaagcggc atgatcagat cgatttcgcc gttcgggtga
     1261 acgtggtgcg gaccggcaat atccttcata tcgaccacgt cgacggaaaa gccgtgggta
     1321 tccggcagcg ccttgaagat ccggccatag cgaatgccgc cgccttcgcg gttgcacagc
     1381 cagccttcgg ccacgcccgt gcggcaggcc tccgtcagcg cgcggaaagt cgcgccatcg
     1441 gcgggatacg tatcgttgag ccacgtgccc agcgcggcgt cgagcggccg gccggcgagt
     1501 tgctctgtca cgcccgcaat caggcgttgg aagtcaggtg gggacatgcg agcctccaga
     1561 tcggcgcggc gcacgcacgt taattcatgc gtgttgctgc gccatttccg tgacgccgcc
     1621 gaaaaatctc gacggcgttt gttattggat caatatttgg cgaaaattaa ccgttcacag
     1681 ggtgtctgtc aagcactata gtacatgtaa cgcagttaat aggtagttaa acatgaacca
     1741 aaattactct cccgcaccgg tagacgaccc tgccgatgag gacgcaagcc gcgccaatcg
     1801 gaccgaccgc gggaccggcg gcgggaccga cggcgggacc gacggcggac ccgagcgcgg
     1861 ggcggagcgt ggcgccgaac gcgaagaacg cgatcccttt ctgacggcga tgggcgatcg
     1921 cgtgcgcttg ctgcgcgcgc gccgcggcat gacgcgcaag acgctggcga ctgaaacagg
     1981 cttgtcggag cgtcacctgg cgaacctcga atcgggtgtg ggtaacgcgt cggtactggt
     2041 gctgcggcag atcgcggcga cgctgaactg cccgttggcc gaggtgatcg gcgacgaaac
     2101 caccgcctcc gctgaatggc tgctgatccg cgaactgctc caggggcgcg accaggcagc
     2161 gttgcagcgc gcccgcgtgg cgcttgccga gatgttcgcg caagcgccgc gcgacccgca
     2221 tcgcaaggat cggatcgcgc tgatcggtct gcgcggggcc ggaaagtcga ccttggggcg
     2281 gatgctggcg caggagcgca aggtgccgtt cgtcgagttg acgcgggtga tcgagcagct
     2341 cgccggctgt ccgccgtcgg agattcattc tctctacggc gcgagcgctt atcggcggta
     2401 tgaacaccgc gcgctcgagg cggtgattca ggagcatgag cgtgccgtga ttgcgtcgcc
     2461 gggcggcctg gtgtcggagt cgggcacctt caacacgctg ctgtcgcact gctacaccgt
     2521 ctggctgcaa gccacgccgg aagagcatat gcgccgtgtt gtcgcgcaag gcgatctgcg
     2581 gccgatgtcc ggcaacaagg aagccatgga tgacctgaag cgcattctcg tggggcgcag
     2641 cgagttgtac ggccgcgccg acatgacctt cgacaccagc gaaaaaacct tggccgacgc
     2701 ctacttgcag ctgcgtgacc ggcttgccgc aaggcttgcg gccgaatcgc cggacgacgc
     2761 acggataggc tgaaatgcag tgcggtgcca gggtttacta gcaaacatgc actaaagtgc
     2821 tttacatggg tttgacgatg cactattgtt caaaaaacag gttttgcatc gtccagtcag
     2881 gagacgcccc atgtccgcag cagaaaccgc aatcgcgccg gtcgactatc gcaccgatcc
     2941 ctcgcagtac aagcattgga agctgagctt caacggtccc gtcgcgaccc tcggtatcga
     3001 catcgccgag gacggcggca tccgtgacgg ctacaagctg aagctgaatt catacgacct
     3061 tggcgtcgac atcgaactgc acgacgcgat acagcgcatc cgcttcgagc atcccgaagt
     3121 cagaacggta gtgctgacga gcctgaagga ccgtgtgttc tgctcgggcg cgaacatctt
     3181 catgctgggt ctgtccacgc atgcgtggaa ggtcaacttc tgcaagttca ccaacgaaac
     3241 acgcaacggt ctcgaagact cgtcgcgtca ttcaggtctg aagtttctcg cggcggtgaa
     3301 cggagcgtgc gcgggcggcg gttatgagct cgcgctcgct tgcgacgaaa tctatctggt
     3361 ggatgaccgc tcgtcgtcgg tgtcgctgcc ggaagtgccg ctgctgggcg tgctgccggg
     3421 cacgggcggc ctgacgcgcg tcaccgataa acgcaaggtg cgtcacgatc gtgcggacat
     3481 cttctgtacg gtggttgaag gcgttcgcgg tgagcgggcg aaagcatggc gtctcgtgga
     3541 tgaagtcgtg aagccgaatc agttcgacca ggcaatccag gcacgcgcgc tcgaactcgc
     3601 cgcccagagc gatcgtcccg cggacgcgca aggcgtcgtg ctgacgcgta tcgaacgcac
     3661 caaccgcgag gacggcctga cctataaaac gctcgacatc acgatcgatc gtgcgaagcg
     3721 catcgccacc tttaccgcga aagccccgca agccggacaa cccacggaga tcgacgcgat
     3781 cgttgccgcc ggcacgagct ggtggccgct gcagttcgcc cgcgaactcg acgacgcgat
     3841 tctgtcgatg cgcaccaacg agctcgacgt cggcacctgg gtcttcagga ccgaaggcga
     3901 cgcccgtaac ctgctcgccg ccgacgccac gctgatgcag cacaaggatc attggttcgt
     3961 acgcgagacc atcggcctct tgcgccgcac gctggcgcgt atcgacgtat cgtcgcgttc
     4021 gctgttcgcg ctgattgaac cgggctcatg ctttgcgggc accttcgcgg aacttgcgtt
     4081 tgcctgcgac cgcagctata tggcggcact gccgagcaac gaagacgaag aaccggcgat
     4141 cacgctctcg gaagtcaact tcggtctcta tccgatggtc acgcatcaat cgcgcctcgc
     4201 tcgccgcttc tatgaagagg ccgaaccgct cgatgcggtg cgttcgaaga tcggccaggc
     4261 gatcaagccg gttgaagccg aacgtttggg ccttgtcacc gcgtcgcccg acgatatcga
     4321 ttgggcagac gaaatccgca tcgcgctcga agaacgcgcg gccatgtcgc cggatgcatt
     4381 gaccggtctg gaagcgaatc tgcgcttcaa cggccctgaa acgatggaga cccgtatttt
     4441 cgggcgtctc accgcatggc agaactggat cttcaaccgg ccgaacgcag tgggcgagaa
     4501 gggcgcgctc aaggtgtacg gcaagggcag caaggcgcaa ttcgacgttt cgcgcgtttg
     4561 attctgttcg acgccacgca actgaacgca acgccgcctg cctgatacgc gcctctcatc
     4621 cgcaagctct gtattccgac cagggaaccg accatgtcaa cgatcaacta cagcgataag
     4681 attccgaaca acgtcaatct cgccgacgat cgcgccttgc aacgcgcact cgaacaatgg
     4741 cagccgaatt ttctgtcgtg gtggggcgat atgggccccg aaggctcgca tggctatgac
     4801 gtgtat
//
LOCUS       DATASH010000600         1652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5045353,
            whole genome shotgun sequence.
ACCESSION   DATASH010000600 DATASH010000000
VERSION     DATASH010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5045353"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..>1652
                     /locus_tag="VHV99_16340"
     CDS             73..>1652
                     /locus_tag="VHV99_16340"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEX3637023.1"
                     /translation="MSTPPKIIYTLTDEAPALATYSLLPIVKAFTRSSDVIVETRDIS
                     LAGRIIAAFPDYLTAEQKGSDDLAELGGLTTRPEANIIKLPNISASVPQLKAAITELR
                     DQGYKLPSYPDVANTDAEKDVKARYDKIKGSAVNPVLREGNSDRRAPLSVKNYARKHP
                     HKMGAWSADSKSHVAHMSGGDFYGSEKSALIAAAGAVKIELTAADGTKTVLKEKTAVQ
                     AGEIIDASVLNKNALRSFIEAEIADAKAKGVLFSVHLKATMMKVSDPIIFGHVVSVFY
                     KDVLTKHADALAQAGFNPNNGIGDLYSRLKDLPEATREAIEADIKAQYAQRPQLAMVN
                     SDKGITSLHVPSDVIVDASMPAMIRESGKMWGADGALHDAKAVIPDRCYADVYQAVIE
                     DCKKNGAFDPVTMGTVPNVGLMAQAAEEYGSHDKTFQISANGVVRVTDAAGTVLIEQA
                     VEAGDIWRMCQTKDAPVQDWVKLAVNRARATNTPAIFWLDAARAHDAQIIRKVEHYLK
                     DHDTSGLDIRVMTPVEAT"
BASE COUNT          365 a          508 c          515 g          264 t
ORIGIN      
        1 cgcttcgtcg caatccgctg gctcagcgct tccggcgctc tgtctctcac ttcacctcgc
       61 aaagcatcaa acatgtccac accgccgaag atcatctaca ccctgaccga tgaagcgcct
      121 gctctggcga cttattcgct gctgccgatc gtcaaggcgt tcacgcgctc gtccgacgtg
      181 atcgttgaaa cgcgcgatat ctcgcttgct ggccggatca ttgctgcatt tccggactac
      241 ctgaccgcgg aacagaaggg ttccgacgat ctggcggaac tgggtggact caccacgcgt
      301 ccggaagcga acatcatcaa gctgccgaac atcagcgctt cggtgccgca actgaaggcc
      361 gcgatcaccg agctgcgcga tcagggctac aagctgccgt cctatccgga cgtggccaat
      421 accgacgctg aaaaagacgt caaggctcgc tacgacaaga tcaagggcag cgccgtgaac
      481 ccggtgctgc gcgaaggcaa ttcggatcgt cgcgcgccgc tgtcggtcaa gaactacgca
      541 cgcaagcatc cgcacaagat gggcgcgtgg agcgcggact cgaagtcgca cgtggcgcac
      601 atgagcggcg gcgacttcta cggcagcgaa aaatcggcgc tgatcgcagc cgccggcgcg
      661 gtcaaaatcg agctgacggc cgctgacggc acgaagacgg ttctgaaaga aaagacggct
      721 gtgcaagcgg gcgaaatcat cgacgcttcg gtgctgaaca agaacgcgct gcgcagcttc
      781 atcgaagcgg aaatcgccga cgcgaaagcg aagggcgtgc tgttctcggt gcacctgaaa
      841 gcaaccatga tgaaggtgtc ggatccgatc atcttcggtc acgtggtgtc ggtgttctac
      901 aaagacgtcc tgaccaagca cgctgacgcg ctggcgcaag cgggtttcaa cccgaacaac
      961 ggtatcggcg acctgtactc gcgcctgaag gacctgccgg aagcaacgcg cgaagcgatc
     1021 gaagccgaca tcaaggcgca gtacgcgcag cgcccgcaac tggccatggt caactcggac
     1081 aagggcatca ccagcctgca cgtgccgagc gacgtgatcg tcgacgcgtc catgccggcc
     1141 atgattcgcg agtcgggcaa gatgtggggc gcggacggcg cactgcacga cgccaaggcg
     1201 gtgattccgg accgttgcta tgccgacgtc taccaggctg tgatcgaaga ctgcaagaag
     1261 aatggcgcat tcgacccggt cacgatgggc acggtgccga acgtcggcct gatggcgcaa
     1321 gccgctgagg aatacggttc acacgacaag acgttccaga tttcggcgaa cggcgtggtc
     1381 cgcgtgaccg acgcagccgg tacggtgctg atcgagcagg cggttgaagc gggcgacatc
     1441 tggcgcatgt gccagaccaa ggacgcgccg gtccaggatt gggtcaagct cgcggtgaac
     1501 cgcgcacgcg ccaccaacac gccggctatt ttctggctgg acgcggctcg cgcgcacgac
     1561 gcccagatca tcaggaaggt cgagcactac ctgaaggacc acgacaccag cggtctggat
     1621 attcgcgtca tgacgccggt cgaagcgacg aa
//
LOCUS       DATASH010000601         9639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6626942,
            whole genome shotgun sequence.
ACCESSION   DATASH010000601 DATASH010000000
VERSION     DATASH010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 9639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9639
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6626942"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2542)
                     /locus_tag="VHV99_16345"
     CDS             complement(<1..2542)
                     /locus_tag="VHV99_16345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX3637024.1"
                     /translation="MTGPDVSVDRRFAPLSRQQLYDAQNVLIITRPPQAPVKPAMGQP
                     GSRSSFVPTEADMFLVVRDDGSVVAFNGHVDLGTGIGTALAQIVAEELDVSLTRVSVV
                     LGHTSEAPNQGPTIASATIQISAVPLRHAAAQARQFLLAEAAARLNVSAEQLDVRDGV
                     VFNRDGDTAKGLGYGDLIAGRRIELTLATDAPLKSPDAYRIVGKSTPRVDIPAKATGE
                     LSFVHDVRVPGMLHGRVVRPPYAGVAQGDFMGNSLLHVDEDSIGDLPGIVKVVVIRDF
                     VGIVAEREEVAQQAVKRLSVQWKAVEGLPPLETSEEVEAALRANPAKRRDLLIQGDID
                     AALAQAPAQTLERTYVWPFQMHASIGPSCAVADYRDEKLKVWSGTQNPHSLRADLALL
                     MALDEAHIEIVRMDAAGCYGRNCADDVAADAALLSRASGAPVRVQLSREDEHAWEPKG
                     AAQLMDVRGALGAEGGLAAYDFATRYPSNDAPTLALLLTGTLCAQPQIFEMGDRTSVP
                     PYDYRTMRIVCDDTPPIVRASWLRGVSALPNTFAHESFIDELAAEAGVDPVEFRLQHL
                     TDPRAIDLVKAVAGKAGWEPRSIASKEVAQQEGDILHGRGFAYARYVHSKFPGFGAAW
                     SAWVADVEVNRKSGELAVTRVVVGQDTGTMVNPDGVRHQIHGNVIQATSRALKERVTF
                     GENAVTSQEWGAYPILTFREVPVIDVVMMPRHGEPPMGTGESASLPGAAAIANALFDA
                     TGVRFRRPPFTPETIRAALADAQAQDAAARKKKRRRFGFLGTIAAGAAGWLGVLAMSP
                     QAIAPITPPLASAFAPELVARGKLLASLGNCAVCHTAHNGVPNAGGKPL"
     gene            complement(2539..3045)
                     /locus_tag="VHV99_16350"
     CDS             complement(2539..3045)
                     /locus_tag="VHV99_16350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX3637025.1"
                     /translation="MNGSTPLTLHVNGATHRVDAGADTPLLYLLRNDLALNGPKFGCG
                     LGECGACTVLLDGMPTRACVTTAKVALGREITTLEGLGTRAALHPVQQAFIEEQAAQC
                     GYCLSGMIMTAKALLDRDPHPSVETIRRELSRNLCRCGTHVEIVRAVQRAAELLAAQP
                     SLRQGVRA"
     gene            complement(3077..4276)
                     /locus_tag="VHV99_16355"
     CDS             complement(3077..4276)
                     /locus_tag="VHV99_16355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3637026.1"
                     /translation="MAAETLTGLSGKVVVTNIGLLLSGDIDQPIFDANTLVIDDGVIV
                     AVGLEKDCDLEGARTTVDCKGTTVAPGLIDSHVHPVFGDWTPRQNQMGWIESNLNGGV
                     TTMISAGEVHLPGRPKDVLGVKALAITAQRSFEGMRGAGVGGGVKVMAGAPVIEKGMV
                     EEDFKELSEAGVKLLGEVGLGSVKGGEEAAQMVAWARKYGIQSTIHTGGPSIPGSGLI
                     DRDVVLAADADVIGHINGGHTSLSYRHVCDLCEQSSRTLEIVHNGNERIALLTARHAI
                     ELKCPHRIILGTDSPAGSGVQPLGILRMIALISSLADVPAEIAFCFATGNTARQRNLR
                     QGLIEVGRPADLVFMDRAQHTAADTLLESVQLGDIPGVGMVMIDGLIRCRRSRNTPPA
                     TEVPVVL"
     gene            complement(4351..5064)
                     /locus_tag="VHV99_16360"
     CDS             complement(4351..5064)
                     /locus_tag="VHV99_16360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3637027.1"
                     /translation="MSEPMLKVSGLNAFYGRAHILFDVGFEVGRGEVVALMGRNGAGK
                     STTMKAVMGLLPRRQGEVRFRGQNITALQPYRIARMGMGFVPEDRRVFADLTVMENLD
                     TGRQPPREGAPEWTPEKLFRLFPNLGEMPKRPGAQMSGGEQQMLTVSRTLMGNPYLVL
                     LDEPSEGVAPVIVEQMANMILELKREGLSILLSEQNLHFAELVSDRAYVLEKGQIRFG
                     GTISELARNETVRRAYLSV"
     gene            complement(5061..5870)
                     /locus_tag="VHV99_16365"
     CDS             complement(5061..5870)
                     /locus_tag="VHV99_16365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3637028.1"
                     /translation="MSLLRVSGLSKSFGGLKAVDNVSFDLEAGQLLALLGPNGAGKST
                     CFNMVNGQLQPSSGSIRLNDHELVGMRPRDIWRLGVGRTFQIAATFNSMTVLENVQMA
                     LVSRERKTFGLWKPAGSRYADEAMTLLEQVGMGADANRACGVLAYGDVKRVELAIALA
                     NRPKLLLMDEPTAGMAPKERNELMALTRRLVTEHKIGVLFTEHSMDVVFAYADRMIVL
                     ARGKLIAEGDAETIRNDARVQEVYFGTGKTFQPRAPLHEAAGGHQGQGALQ"
     gene            complement(5867..7807)
                     /locus_tag="VHV99_16370"
     CDS             complement(5867..7807)
                     /locus_tag="VHV99_16370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3637029.1"
                     /translation="MLSNLLVQLVNGLADASTLFLVAAGLSLIFGVTRIVNFAHGSFY
                     MFGIYVGYSMASRFGQTAGGFWLSVLAAALVVAVLGALVEMLVLRRIYQAPELFHLLA
                     TFALVLIFRDAALWLWGPEDLFGPRAPHLAGAVDFLGHPLPTYDIALIVIGPVVLLLL
                     WYALTRTRWGTLVRAATQDREMLGALGINQAWLFTGVFFVGAFLAGLGGALQGPRMSA
                     NLSLDLETIGNAFVVVVVGGMGSIPGAFVAALLIAEIKALCIGIGHVSVFGIDLSLSR
                     FTLVAEFVVMAVVLVVRPWGLLGKASAAVRGMAAPEAPLRPAGKRLKWLAAIALLGLV
                     LAPLAANAFPYMPVLLVEILIAVLFATSLHFIMGPGGMHSFGHAAYFGLGAYGAALFL
                     KVLNLPMEAALLLGPLLAVAGALVFGWFCVRLSGVYLAMLTLAFAQIVWSVVFQWDDV
                     TGGSNGILGLWPSNWLSSPVAFYYLTLACAVLGVWLLRKMLFSPLGYAMRASRDSVLR
                     AEAIGIDVKRVQWAAFVVASLFCGLAGSLYAFSKGTISPEVISVSRSVDGLVMVLLGG
                     LQTLTGPIVGAAVFTWLQDTVARQTDYWQALLGFAILLLVIAFPQGIVGFIRERFGDD
                     NADRAEKSPTSRSQGAAIKEGL"
     gene            complement(7826..8410)
                     /locus_tag="VHV99_16375"
     CDS             complement(7826..8410)
                     /locus_tag="VHV99_16375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018423562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid synthesis family protein"
                     /protein_id="HEX3637030.1"
                     /translation="MAIKLRKLVVQVDETRIEMGQAIEPPARRAVAIAVIDNPYAGRY
                     EATLDALIEAGEELGALLGNKCVEALGIKPGDAQSYGKAAIVGEAGELEHAAAILHPK
                     LGAPLRVAVEKGAALVPSAKKMGTLGTAIDVPLGHKDAAFVRSHFDAIEARVSDAPRA
                     NEIVVAVAVTASGRPLPRIGGLQVSEIKGEDGLR"
     gene            complement(8447..9037)
                     /locus_tag="VHV99_16380"
     CDS             complement(8447..9037)
                     /locus_tag="VHV99_16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid synthesis family protein"
                     /protein_id="HEX3637031.1"
                     /translation="MFEIRRVLTHVEDIFHEFGPAPAQPLRRGAIAAVMTNPFAGRYE
                     ARIEHAMEALKPIGFDMAQRLLAAMAVPHASIESYGKGAIVGSRGELEHGALWHVPGG
                     YAMRELLEKNGVPTNAIVPSTKKVGAPSTALDVPLTHVNASYVRSHFDAIEVRVPGAP
                     AADELVYILVMSTGQRVHARVGGLAKEAIVGKDGLR"
     gene            complement(9013..>9639)
                     /locus_tag="VHV99_16385"
     CDS             complement(9013..>9639)
                     /locus_tag="VHV99_16385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HEX3637032.1"
                     /translation="DELITAFAREGISRAFINNGGDIALHLTEGQQYRVGVFADLAQF
                     SAAGLSGVQALDANLTLDAAMPIRGVATSGWRGRSFSLGIADSVTVLARNAATADAAA
                     TMIANAVNLEHAGIVRRPASSLKDDSDLGDMLVTVDVPSLPQPLIDFALARGVHAARR
                     LQEQGLIEGAALFLQGRARVADTHHDGALFMNRAEIATEAPCSKYAAC"
BASE COUNT         1802 a         3290 c         2900 g         1647 t
ORIGIN      
        1 cgagcggttt accgcccgcg ttcggcacgc cgttgtgcgc ggtatggcaa accgcgcaat
       61 tgcccagcga cgcaagcagt ttgccgcgcg ccaccagttc gggcgcgaat gcgctcgcaa
      121 gcggtggcgt aatcggcgca atggcctgcg gcgacatcgc caacacaccc aaccaaccgg
      181 ccgcgcccgc ggcgatggtg ccaagaaagc cgaaccgccg acgcttcttc ttgcgcgccg
      241 ccgcgtcttg cgcttgcgca tcggcgagcg cggcgcggat cgtctccggt gtgaacggcg
      301 gtctgcgaaa ccgcacacct gtcgcgtcga acagcgcgtt ggcgatcgcc gcggcgccag
      361 gcagcgacgc cgattcgccc gtgcccatcg gcggctcgcc gtggcgcggc atcatcacca
      421 cgtcgatcac cggcacttca cggaacgtca gaatcggata cgcgccccac tcctggctcg
      481 tgaccgcgtt ctcgccgaac gtcacgcgct ctttcagcgc ccggctggta gcctggatca
      541 cattgccatg tatctgatga cgcacgccgt caggattcac catcgtgccg gtgtcctggc
      601 cgaccaccac gcgcgtcacg gcaagctcgc cgctcttgcg attcacttcg acgtccgcga
      661 cccacgcgga ccatgccgcg ccgaagccgg ggaatttgct gtgcacgtag cgggcgtaag
      721 cgaagcctct gccgtgcaga atgtcgcctt cttgctgagc aacctctttc gaagcaatgc
      781 tgcgaggctc ccaaccggct ttcccggcca ccgccttgac cagatcgatc gcgcgcggat
      841 cggtcagatg ctgcaaacga aactcgaccg gatcgacgcc cgcttcggcc gcgagttcgt
      901 cgatgaaaga ctcatgcgcg aacgtgttcg gcaacgcgga cacgcctctt aaccacgacg
      961 ctcgcacgat cggcggcgtg tcgtcgcaaa cgatgcgcat ggtacggtaa tcgtacggcg
     1021 gcacggacgt gcggtcgccc atttcgaata tttgcggctg tgcgcagagg gttccggtga
     1081 gcagcagcgc tagtgtgggc gcgtcgttcg atggatagcg cgtcgcgaag tcgtatgccg
     1141 cgaggccgcc ttcggcacct aaagcaccac gcacatccat caattgcgct gcgcccttcg
     1201 gctcccatgc gtgctcgtct tcgcgcgata gttgcacgcg caccggagcg ccgcttgcgc
     1261 gagataacag cgcggcatcg gcggcgacgt cgtcggcgca attgcggccg tagcagcccg
     1321 ccgcgtccat gcggacgatt tcgatgtgcg cttcgtcgag cgccatcagc aaggcgagat
     1381 ccgctcgtaa cgaatgggga ttctgcgtgc ccgaccagac tttcagcttt tcatcgcggt
     1441 aatcggccac cgcgcacgac ggtccaatcg acgcatgcat ctgaaacggc catacgtagg
     1501 tgcgttccag cgtttgggcc ggggcttgcg cgagtgctgc gtcgatatcg ccctggatca
     1561 gcagatcgcg tcgtttggcg ggattggctc tgagcgcggc ttccacctcc tcgctggttt
     1621 cgagcggcgg caaaccttcc acagctttcc actgcacgct caagcgtttg acggcttgct
     1681 gcgcgacctc ctcgcgctcg gccacgatgc cgacgaaatc gcgaatcacc accaccttga
     1741 cgatgcccgg caaatcgccg attgaatcct catcgacatg cagcaggcta ttgcccatga
     1801 agtcgccctg cgccacaccg gcatacggag gccgtacaac gcgcccgtgc aacatgcccg
     1861 gcacacgcac gtcatgtacg aaactcaatt caccggttgc cttcgccggg atatcgacgc
     1921 gcggcgtact cttgccgacg atcctgtacg cgtccggcga tttcaacggg gcatcggtgg
     1981 cgagcgtcaa ttcgatgcgc cgtcccgcga tcaggtcgcc gtagccaagg ccttttgccg
     2041 tgtcgccgtc gcgattgaaa accacaccgt cgcgcacgtc gagttgctcc gcgctgacgt
     2101 tcaatcgcgc agccgcttcc gccagcagga attgccgcgc ttgcgcggcc gcgtgccgta
     2161 acggcaccgc cgatatctgg atcgtcgcgc tggcaatcgt cggcccctga ttcggcgctt
     2221 cgctggtgtg gccgagcacg accgacacgc gcgtcaacga cacatccagc tcttccgcga
     2281 cgatctgcgc gagcgccgtg ccgatgccag tgccgagatc cacatggccg ttgaacgcca
     2341 ccacgctgcc gtcgtcgcgc accacgagaa acatgtccgc ctcggtcggc acgaacgacg
     2401 aacgcgaccc gggctgaccc atcgctggct ttacaggcgc ctgcggcggc cgcgtgatga
     2461 tgagcacatt ctgcgcgtcg taaagctgct gtcgcgacag cggcgcgaaa cgtcgatcga
     2521 cgctgacatc cggccccgtc atgcgcgcac tccttgacgc aaggaaggtt gcgcggcaag
     2581 caattcggcc gcgcgctgca ccgcgcgtac gatctcgaca tgcgtgccgc agcggcacag
     2641 attgcgcgac aactcccgcc ggatcgtctc gacgctcgga tgcggatccc gatcgagcaa
     2701 cgccttggcg gtcatgatca tgccgctcag gcaatagccg cattgggcgg cctgctcttc
     2761 gatgaacgcc tgctgcaccg gatgcagtgc cgcacgcgtg cccaatcctt cgagcgtggt
     2821 gatctcgcgg cccaatgcaa ccttcgcggt cgtcacgcaa gcgcgcgtcg gcatgccgtc
     2881 gagcagcacc gtgcacgcgc cgcattcgcc gaggccgcag ccgaatttcg gaccgttcaa
     2941 cgcgaggtcg ttacgcagca gataaagcag tggcgtgtcg gcgccggcgt cgacgcgatg
     3001 cgtcgcgccg ttgacgtgca gcgtaagagg cgtcgaaccg ttcatgaaag tgccggccac
     3061 gccgtgcggt gcgcgttcaa agcaccaccg gcacttcggt cgccggtggt gtattacggc
     3121 tgcgccggca gcgaatcaaa ccgtcgatca tcaccatgcc gacaccggga atatcgccga
     3181 gctgcacgct ttccagcaac gtatcggccg ccgtgtgctg cgcgcgatcc atgaacacga
     3241 gatcggcggg acggcccact tcaatcaaac cctggcgcaa gttgcgttgc cgcgcggtgt
     3301 tgccggtcgc gaagcagaac gcgatttcgg cgggcacgtc cgccagactg gaaatcagcg
     3361 cgatcatccg cagaatgccg agcggctgaa cgccggaacc cgccgggcta tcggtgccga
     3421 gaatgatccg atgcggacac ttcaattcga tcgcgtgacg cgcggtgagc agcgcgatcc
     3481 gctcattgcc gttgtgaacg atttccagcg tgcggctcga ttgctcgcac agatcgcata
     3541 cgtgccgata ggacagcgac gtatggccgc cgttgatgtg gccgatcacg tccgcgtcgg
     3601 cggccagcac cacgtctcta tcgatcaggc ccgaacccgg aatcgacggg ccacccgtgt
     3661 ggatcgtgct ttgaatgccg tatttgcgtg cccatgcgac catctgggcc gcttcctcgc
     3721 cgcccttcac gctgccgaga ccgacttcgc ccagcagctt cacgcctgct tccgagagct
     3781 ccttgaagtc ctcttcgacc atgcccttct cgatcaccgg cgcacccgcc atcactttca
     3841 cgccgccgcc gacacccgcg ccgcgcatgc cctcgaacga acgctgcgcg gtgatcgcga
     3901 gcgccttgac gccgagcacg tctttcggac ggccgggcaa gtgcacttcg ccggcggaga
     3961 tcatcgtcgt cacgccgcca ttcagattcg attcgatcca gcccatctga ttctggcgcg
     4021 gcgtccagtc gccgaacacc ggatgaacgt gcgaatcgat caggcccggc gcgaccgtgg
     4081 tgcctttgca gtcgacggta gtgcgtgcgc cttcgaggtc gcaatccttc tccaggccaa
     4141 ccgcgacgat cacaccgtcg tcgatcacca gcgtgttcgc gtcgaaaatc ggctggtcga
     4201 tatcgccgga taagagtagc ccgatgttcg ttaccacgac cttgcccgac aagcccgtca
     4261 acgtctctgc tgccatctgc ttctcctttc gcaacgacta ccgggcgccg tgtctcgtgc
     4321 acacgctgca cgtctcatgc gccgatggaa tcacacgctc agataagcgc gcctcactgt
     4381 ttcgttcctt gcgagttcgc tgatcgtgcc gccgaaacgg atctgtcctt tttcgaggac
     4441 atacgcgcgg tcgctcacca gctcggcgaa gtgcaggttc tgttcggata acagaatcga
     4501 gagcccttcg cgcttgagtt cgagaatcat gttcgccatc tgttcgacga tcacgggtgc
     4561 aacgccctcg gagggttcgt cgagcagcac cagatacgga ttgcccatca gcgtgcgcga
     4621 gacggtgagc atctgctgct cgccaccgct catctgggcg cctgggcgct tcggcatttc
     4681 gccgaggttg gggaagagcc ggaacagttt ttccggcgtc cattcaggcg caccttcgcg
     4741 cggcggttga cggcccgtat cgaggttctc catcacggtc agatcggcga acacgcggcg
     4801 atcctcgggg acgaaaccca tgcccatgcg cgcgatacga tacggctgca acgccgtgat
     4861 gttttgcccg cgaaaacgca cctcgccctg acggcgcggc agcagaccca tcaccgcttt
     4921 catcgtcgtc gacttgccgg cgccgttgcg gcccatcagc gcgaccactt caccgcggcc
     4981 gacctcgaag ccgacgtcga acagaatgtg cgcgcggcca tagaacgcat tgaggccgga
     5041 cactttgagc atcggctcgc tcattgcagc gctccctgtc cttgatggcc gccggccgct
     5101 tcatgcagcg gcgcgcgtgg ctggaaggtc ttgccggtgc cgaaatagac ctcctgcacg
     5161 cgcgcgtcgt tgcggatcgt ttcagcgtcg ccctcggcga tcagcttgcc tcgcgcgagc
     5221 acgatcatgc gatcggcgta ggcaaatacc acgtccatgc tgtgctcggt aaacagcacg
     5281 ccgattttgt gctcggtgac gaggcgcctg gtcagcgcca tcaactcgtt gcgctccttg
     5341 ggtgccatgc cggcagtggg ctcgtccatc agcagcaact tcgggcggtt tgccagcgcg
     5401 atagcgagtt cgacgcgttt gacgtcaccg taggcgagca cgccgcaggc gcggttcgcg
     5461 tcggcgccca tgccgacctg ctcgagcagc gtcatggctt cgtcggcgta gcgcgaaccc
     5521 gcgggtttcc acaggccgaa ggtcttgcgt tcacgcgaga ccagcgccat ctgcacgttc
     5581 tcgagcacgg tcatcgaatt gaaggtcgcg gcaatctgaa acgtgcggcc gacgccaaga
     5641 cgccagatat cgcgtggccg catgccgacc agttcgtgat cgttcagccg gatcgacccg
     5701 gacgacggct gcagttgccc gttgaccatg ttgaagcagg tcgacttgcc cgcgccattt
     5761 gggcccagca gggccagcag ttgaccggct tcgagatcga aggagacatt gtcgaccgcc
     5821 ttcaggccgc cgaacgactt ggagaggccg gacacgcgca gcaaactcat agcccctcct
     5881 tgatcgcagc gccttggctg cgtgacgtgg ggctcttttc ggccctatcc gcgttgtcgt
     5941 cgccgaaacg ctcacgaatg aatccgacga taccctgcgg aaacgcaatc accagcagca
     6001 ggatcgcgaa gcctagcagc gcctgccaat aatcggtttg ccgcgcgacg gtgtcctgca
     6061 gccacgtgaa cacggccgcg ccgacgatcg ggccggtcaa cgtttgcaag ccgccgagca
     6121 gcaccatcac gaggccgtcc acggagcggc tgacgctgat cacttccggc gaaatcgtcc
     6181 ctttggagaa ggcataaagc gatccggcga gcccgcaaaa tagcgacgcg acgacgaacg
     6241 ccgcccactg cacgcgtttc acgtcgatgc cgatcgcctc ggcgcgcagc accgaatcgc
     6301 gcgacgcccg catcgcatag ccaagcggcg agaacagcat cttgcgcagc agccacacgc
     6361 cgagcacggc gcaggcgagc gtcaaataat aaaaagcgac cggcgacgac aaccagttcg
     6421 acggccacaa gccgagaatg ccgttactgc cgcccgtcac gtcgtcccac tggaacacga
     6481 ccgaccagac gatctgcgca aacgcaagcg tcagcatggc gagatagacg cctgagagcc
     6541 gcacgcaaaa ccagccgaac accagcgcgc cagccaccgc aagcaagggg ccgagcagca
     6601 acgcggcttc catcggcaga ttgaggactt tgagaaacaa cgcggcgcca tatgcgccga
     6661 ggccgaagta cgcggcgtgg ccgaacgaat gcatgccgcc agggcccatg atgaaatgca
     6721 gactcgtcgc gaacaggacg gcgatcagaa tttcaaccag cagcacgggc atgtagggaa
     6781 acgcattggc cgcaagcggc gcgagtacca accccagcaa cgcgatggcc gccagccact
     6841 tcaggcgctt gccggcgggc cgcaatggcg cttcaggggc ggccatgccc cgcaccgcgg
     6901 cgctcgcctt ccctaacaaa ccccaaggcc gcaccaccag cacgaccgcc atcacgacaa
     6961 attccgcaac gagcgtgaaa cggctcaacg acagatcgat gccgaacacc gacacatgcc
     7021 caatgccgat gcacaatgcc ttgatctccg cgatcagcaa tgccgcgacg aacgcgcctg
     7081 gaatcgaccc catgccgccg accaccacca ccacgaacgc attgccgatg gtctccagat
     7141 cgagcgacag attcgccgac atccgcggtc cttgcaacgc gccgcccaaa ccggcgagaa
     7201 aagcgccgac gaaaaacacc ccggtgaaca gccacgcctg attgatgccg agcgcgccga
     7261 gcatctcgcg gtcctgcgtc gcggcgcgca cgagcgtgcc ccaacgcgtg cgcgtcaacg
     7321 cgtaccacag cagcagcaac accaccggcc cgatcacgat cagcgcgata tcgtaggtcg
     7381 gcagcggatg gccgagaaaa tcgactgcgc cggccagatg cggcgcacgc ggtccaaata
     7441 ggtcttccgg gccccacagc cacagcgcgg cgtcgcggaa aatcagcacc agcgcgaatg
     7501 tcgccagcag gtgaaacaac tcgggggcct gatagatgcg ccgtaacacg agcatttcga
     7561 ccaacgcgcc gagcacggca accaccaacg cggcagccag caccgacagc cagaatccgc
     7621 ccgccgtctg gccgaagcgg ctcgccatgc tatacccgac atagatgccg aacatataga
     7681 acgagccgtg cgcgaagttg acgatgcgcg tcacgccgaa gatcaacgac aaaccggcgg
     7741 cgacgagaaa cagcgtcgac gcgtcggcga gtccgttgac cagctgtacc agcaaattcg
     7801 aaagcatcgc gacccttaag aaaactcagc gcaaaccgtc ttcgcccttg atctcactca
     7861 cctgcaagcc gccgatgcgt ggcagcggcc ggccggaagc cgtcaccgcg accgccacga
     7921 cgatttcatt cgcgcgcggc gcgtccgaca cccgcgcttc gatggcgtcg aaatgactgc
     7981 ggacgaaggc cgcgtccttg tggccgagcg gcacgtcgat cgcggtgccg agcgtcccca
     8041 tttttttcgc cgagggcacc agcgccgcgc ctttttcaac cgcgactcgc aaaggagcgc
     8101 cgagcttggg atgcaggatc gccgccgcgt gttccagttc gcccgcctcg ccgacgatcg
     8161 ccgccttgcc gtagctttgc gcgtcgcccg gcttgatgcc tagcgcttcg acgcacttgt
     8221 tgccgagtaa cgcgcccagc tcctcaccgg cttcgatcaa cgcgtcgagc gtggcctcgt
     8281 agcggcccgc atagggattg tcgatcaccg caatcgcgac tgcccggcgt gccggcggct
     8341 cgatggcctg gcccatttca atgcgtgttt catcgacctg caccaccagc ttgcgaagct
     8401 tgattgccat ttcgatctcc gtgtttcgat gcgtgttctg cgcctttcag cgcaatccgt
     8461 ccttacccac aatcgcttct ttcgccaacc cgccgacgcg cgcatgcacg cgctggcccg
     8521 tgctcatcac gaggatatac accagttcgt ccgcggcggg cgcaccgggc acacgcacct
     8581 cgatcgcgtc gaaatggctg cgcacgtagc tcgcgttgac atgcgtcagc ggcacgtcga
     8641 gcgcggtgga aggcgcgccg actttcttgg tcgacggcac gatggcgttg gtcggcacac
     8701 cgtttttctc gagcaattcg cgcattgcat agccgccggg gacatgccac agcgcgccat
     8761 gctcgagttc gccgcgcgag ccgacgatgg cgcccttgcc gtaactttcg atcgatgcgt
     8821 gcggcacggc cattgccgcc agcagccgct gcgccatatc gaagccgatc ggcttgagcg
     8881 cttccatggc gtgttcgatt ctggcttcgt agcggccggc aaacggattc gtcatcaccg
     8941 cggcaatcgc gccgcgccgc agcggttgcg cgggcgcggg gccgaactcg tggaaaatgt
     9001 cttcgacgtg cgtcagcacg cggcgtattt cgaacacgga gcctccgttg ctatttcagc
     9061 gcggttcatg aacaacgcgc catcgtgatg agtatcagcc actcgcgccc gtccttgcag
     9121 gaacagtgcg gcgccttcga tcaaaccttg ttcctgtaaa cgccgggcgg cgtggacgcc
     9181 gcgtgccagc gcaaaatcga tcagcggttg aggcaaggac ggcacatcga cagtcacgag
     9241 catgtcgccg agatcgctgt cgtccttcaa ggaggaagcc ggcctgcgca cgatgcccgc
     9301 atgctcaaga ttgacggcgt tggcgatcat cgtcgcggcg gcatcggcgg tggcggcgtt
     9361 gcgggccagc acggtcacgc tgtcggcaat cccgaggctg aagctgcgcc cgcgccagcc
     9421 gcttgtcgcg acaccgcgga tcggcatcgc ggcgtcaagc gtcaggttgg cgtccaaggc
     9481 ctgaacgcct gacagcccgg cagcggagaa ctgcgccaga tcggcgaaca cgcctacgcg
     9541 gtattgctgc ccttcggtca aatggagcgc gatgtcgccg ccgttgttga tgaaggcgcg
     9601 cgatatgccc tcgcgcgcga acgccgtgat caattcgtc
//
LOCUS       DATASH010000602         3066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_691380,
            whole genome shotgun sequence.
ACCESSION   DATASH010000602 DATASH010000000
VERSION     DATASH010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691380"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VHV99_16390"
     CDS             <1..258
                     /locus_tag="VHV99_16390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012329425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX3637033.1"
                     /translation="VEDTRSHLKNYRRLGIRTVWITGHLPRKPHADGVPTRLPGTGRP
                     HYVDRSIRSLKSLRLGTRSVRLKGQQTGRQPCSRSTNLTKP"
     gene            225..926
                     /gene="slmA"
                     /locus_tag="VHV99_16395"
     CDS             225..926
                     /gene="slmA"
                     /locus_tag="VHV99_16395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HEX3637034.1"
                     /translation="MQPIDKPDEAVAEHEPTPPPATSRATRLKPGERRVHILQTLAAM
                     LEAPKSEKITTAALAARLGVSEAALYRHFASKAQMFEGLIEFIEQTLFGLINQIADKE
                     SNGVLQARAIALMLLNFPAKNPGMTRVLTCEALVGEHERLTERVTQMLERVEASLKQC
                     LRLAQMEASESAGENASPSANVHAAPLPAAYDPAIRASLLLSYIIGRWHRYVRSGFVR
                     PPAEHADAQLLLILQ"
     gene            1241..2386
                     /locus_tag="VHV99_16400"
     CDS             1241..2386
                     /locus_tag="VHV99_16400"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006761998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEX3637035.1"
                     /translation="MESIGIVAPQKMHFTEPLLMQNGSSLAGYDLMVETYGTLNAARS
                     NAVLVCHALNASHHVAGVYADNPKDIGWWDNMVGPGKPLDTNRFFVIGVNNLGSCFGS
                     TGPMSIDPATGIPYGASFPVVTVEDWVNAQVRVADEFGITRFAAVMGGSLGGMQALAW
                     SMMYPERVGHCIVVASTPKLSAQNIAFNEVARSAILSDPDFHGGNYYAHNVKPKRGLR
                     VARMIGHITYLSDDDMAEKFGRSLRRAEGALDAYNFNFDVEFEVESYLRYQGDKFADY
                     FDANTYLLITRALDYFDPAKAFDGDLTAAVAHTTAKYLIASFSTDWRFAPARSRELVK
                     ALLDHKRTVTYAEIDAPHGHDAFLLDDARYHNLMRAYYERIANEVNA"
     gene            2383..2991
                     /gene="metW"
                     /locus_tag="VHV99_16405"
     CDS             2383..2991
                     /gene="metW"
                     /locus_tag="VHV99_16405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEX3637036.1"
                     /translation="MNQRALDYLALRPDFRAIARWVEPRATVLDLGCGDGSLLSLLTE
                     ELDVQGYGIEINDAGVLASTQNGVNVIQQNLEDGLRLFEDSSFDFAVLSQTLQTIHQT
                     AAILRETVRVGKECIVSFPNFGYWAHRLSVLQGRMPVSKSLPYQWHNTPNVRVLTIKD
                     FEALAPEVGIEILDRVVLHDGQVVRWGVNWRGSLAVYRVKKS"
BASE COUNT          599 a          988 c          928 g          551 t
ORIGIN      
        1 gtggaagaca cgcgctcgca tctgaagaac taccggcggc tcggcatccg taccgtgtgg
       61 atcaccggcc atctgccgcg caagccgcat gcggacggcg tgccgacgcg tctgcctggc
      121 accggccggc cgcattatgt cgaccgaagc attcgttcgt taaaatcgct acgactgggc
      181 acccgctcgg ttcgattgaa gggacagcag acgggacgac agccatgcag ccgatcgaca
      241 aacctgacga agccgtagcg gaacacgaac ccacgccgcc gcccgccacg tcgcgcgcga
      301 ctcgcctgaa gccgggcgag cgtcgcgtgc acatcctgca gacgcttgcc gccatgctgg
      361 aagcgccgaa aagcgaaaag atcactacgg cggccctcgc cgcccggctc ggcgtctcgg
      421 aagccgcgct gtaccgtcat ttcgccagca aggcgcaaat gttcgaaggg ctcatcgagt
      481 tcatcgagca gaccctcttc ggcctgatca accagatcgc cgacaaggag agcaacggcg
      541 tcctgcaagc ccgtgcgatc gcgctgatgc tcctcaactt cccagcgaaa aatcccggca
      601 tgacgcgcgt gctgacgtgc gaggcgctgg tcggcgagca cgaacggctg accgagcggg
      661 tcactcagat gctggagcgg gtcgaggcgt cgttgaaaca gtgtttgcgg ctggctcaga
      721 tggaagcaag cgaaagcgcg ggggaaaacg cgagcccgag cgccaatgtg catgctgcgc
      781 cgctccccgc cgcctacgat cccgcgatcc gcgcgagcct gctgttgagt tacatcatcg
      841 gccgctggca tcggtatgtg cgcagcggct ttgtgcggcc gcccgctgaa cacgccgatg
      901 cacaactgct gctgattctt cagtagtccg cgcggcatgc cagctcgcag agccgctcgc
      961 gacgctgccg cacgaaattt tccgctatac tttgcgcctg actacggacg ctcgcaccac
     1021 ctgagaccgc agtcctgagc accttgaaca cccttgaaca cccttgaaca ctatcacgcg
     1081 ccgatttgct gactcgattg cgggcgcgcg aactgccggg aacgtctccc gcggttcact
     1141 acaaatgcgg ctaaagaggt cgtcagccgc gcacactgtt tctcctccgt gctttgccga
     1201 cgccatctag ccgccctgtt tttgttaaac ggcggaatga atggaatcga tcggtatcgt
     1261 cgctccccaa aaaatgcatt tcaccgagcc gctgctcatg cagaacggca gctcgctggc
     1321 cggttacgat ctgatggtcg aaacctacgg cacgctcaac gccgcgcgca gcaacgccgt
     1381 gctggtatgc cacgcgctca atgcgtcgca ccacgtggcg ggcgtgtatg cggacaatcc
     1441 gaaggacatc ggctggtggg acaacatggt tggtccgggc aaaccgctcg acaccaacag
     1501 gttcttcgtg atcggtgtga acaatctggg ctcatgcttc ggctcgaccg gcccgatgag
     1561 cattgatccg gcgacgggca ttccgtacgg cgcgagcttt ccagttgtga ccgttgaaga
     1621 ctgggtgaac gctcaagtgc gcgtcgccga tgagttcggc atcacacgtt tcgccgccgt
     1681 gatgggcggc agcctcggcg gcatgcaggc gctcgcgtgg agcatgatgt atccggagcg
     1741 cgtcggccat tgcatcgtgg tcgcgtccac gccgaagctg tcggcgcaga acatcgcgtt
     1801 caacgaggtg gcgcgctcgg cgatcctgtc ggaccccgac ttccacggcg gcaactacta
     1861 cgcgcacaac gtcaagccga agcgcggctt gcgcgtcgcg cggatgatcg gccacatcac
     1921 ctatttgtcc gacgacgaca tggccgagaa attcggccgc tcgttgcgcc gcgcggaagg
     1981 cgcgttggac gcgtacaact tcaacttcga cgtggaattc gaagtggagt cctacctgcg
     2041 ctaccaaggc gacaaattcg ccgactactt cgacgcgaac acgtatctgc tgatcactcg
     2101 cgcgctcgac tatttcgatc ccgccaaagc cttcgacggc gatctgaccg ccgctgtcgc
     2161 gcacaccacg gcgaaatacc tgatcgccag cttctcgacc gactggcgtt tcgcgccggc
     2221 ccgctcacgc gaactggtga aggcgctgct cgaccacaag cgcacggtga cctacgcgga
     2281 gatcgacgcg ccgcacggcc acgacgcctt tctgctcgac gacgcgcgtt atcacaatct
     2341 gatgcgcgcc tattacgaac gtattgcgaa cgaggtgaac gcatgaacca gcgagcactc
     2401 gattacctgg ccctgcgccc ggacttccgc gcgatcgccc gttgggtcga accgcgcgcc
     2461 accgtgctcg acctgggttg cggcgacggc tcgctgttgt cgctgctgac ggaagaactg
     2521 gacgtgcagg gttacggcat cgagatcaac gacgccggcg tactggcgtc gacgcaaaac
     2581 ggcgtcaacg tgatccagca gaatctggaa gacggcctgc gcctgttcga agacagcagc
     2641 ttcgactttg cggttctgtc gcaaacgctt caaaccattc atcagacggc tgccatcctg
     2701 cgcgagacgg tgcgcgtggg caaggaatgc atcgtgtcgt tcccgaattt cggctactgg
     2761 gcgcaccggc tttccgtcct gcagggtcgc atgccggtgt cgaagtcgct gccttatcag
     2821 tggcacaaca cgccgaacgt gcgggtgctg accatcaagg atttcgaggc actggccccg
     2881 gaagtcggta tcgaaatact cgaccgcgtg gtgcttcacg atggccaggt ggtgcggtgg
     2941 ggggtgaact ggcgtggtag tcttgcggtc tatcgcgtca agaaaagcta acgcaagtta
     3001 tccacatgtc gaacccgccg cacgaggcgc ctgcacttac cgctcacgaa gaacatcccg
     3061 gctggc
//
LOCUS       DATASH010000603         8235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2671229,
            whole genome shotgun sequence.
ACCESSION   DATASH010000603 DATASH010000000
VERSION     DATASH010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 8235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8235
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2671229"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            382..561
                     /locus_tag="VHV99_16410"
                     /pseudo
     CDS             382..561
                     /locus_tag="VHV99_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917337.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            685..947
                     /locus_tag="VHV99_16415"
                     /pseudo
     CDS             685..947
                     /locus_tag="VHV99_16415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420346.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            1622..1726
                     /locus_tag="VHV99_16420"
                     /pseudo
     CDS             1622..1726
                     /locus_tag="VHV99_16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612010.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            2111..3454
                     /gene="hemA"
                     /locus_tag="VHV99_16425"
     CDS             2111..3454
                     /gene="hemA"
                     /locus_tag="VHV99_16425"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEX3637037.1"
                     /translation="MNLLSIGINHHTAPVALRERLAFPLEQIRPALQTLKAAWLRRDS
                     ARFSTAHTPEPVERLASSGNALSEAAILSTCNRTEIYCATDDRAAPEHAIAWLSAYHG
                     IAVDEFVPHVYTLPQSEAVRHAMRVASGLDSMALGETQILGQMKNAVRAASEAGAMGT
                     YLNQMFQRTFSVAKEVRDQTEIGAHSVSMAAAAVRLAQRIFDEISSQRVLFIGAGEMI
                     GLCATHFAARRPRELVVANRTPERGSALAKRVGGQAIALVDLPERLHEFDIVVSCTAS
                     SLPIIGLGAVERAIKARKHRPMLMLDLAVPRDIEQEVEQLGDVYLYTVDDLAEIVREG
                     AATRQAAVAQAEAIIETRVQGFMRWLEQRSVVPVIRHMHTQADMLRRVELERAQKMLA
                     RGDDPAVVLEALSQALTNKFIHGPTNALTHVHADNRDKLVDLFSGFYHHPGPSGG"
     gene            3593..3787
                     /locus_tag="VHV99_16430"
                     /pseudo
     CDS             3593..3787
                     /locus_tag="VHV99_16430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3974..5173
                     /locus_tag="VHV99_16435"
     CDS             3974..5173
                     /locus_tag="VHV99_16435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011694206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX3637038.1"
                     /translation="MLAAIGTTAAHAQSSVTLYGLLDAGISYTSNTRVGTTGHSNVAL
                     SNAMMQSSRWGLRGSEDLGGGLKAVFVLENGFTLTNGKLGQGGREFGRNAYVGLSSNA
                     LGTLTVGRQNEFMYDWVSRVSMENFNGAGGNVFAHALDNDNMLGTFRVNNAVKYQSAN
                     YAGLTFGGLFGFSNQAGGFGNNRAYSFGAMYNNGPILATLGYTQVNNSLTNSTAGLGV
                     VTNTGGAVDSSAASTSADAALTAGRQRIFGGGLNYTFGPAKVGFVITDSKLDNATGFT
                     SGTSALSLANSAYVHFTNYEVNAHYNLTPALTLGAAYTYTDGKFSNAQNSVSPKWSQV
                     SLSSDYNLSKRTDVYAVANWSHLSGGLSAQAGSGLAAFEGASSYIGGIAATKNQVVVG
                     AGMRTRF"
     gene            complement(5690..5977)
                     /locus_tag="VHV99_16440"
     CDS             complement(5690..5977)
                     /locus_tag="VHV99_16440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637039.1"
                     /translation="MKYMTIAIAFAGTLSATAAFAQSTPSALSVNPRPATRAVQIASN
                     TTPVASPAGQPVAPSGQPLAGKTRAQVYQELVHAEKDGQFAYLYSTIYAGG"
     gene            complement(6288..6620)
                     /locus_tag="VHV99_16445"
     CDS             complement(6288..6620)
                     /locus_tag="VHV99_16445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637040.1"
                     /translation="MSPHARSVPARLLRGHTGIGDDPKGPLFRTIRRCIGQLGTTPLP
                     QANACAMVRRCALVAGIGTKIRNHTFRATGSGAFLKNGGTLENAAAGEPLIDAYDPAV
                     MTGTMRSA"
     gene            complement(6681..6842)
                     /locus_tag="VHV99_16450"
     CDS             complement(6681..6842)
                     /locus_tag="VHV99_16450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637041.1"
                     /translation="MLAATEARQLLYSIDVTTPTGLRDRALIAPMVLSVLSFARVGAA
                     LAMRVEDVL"
     gene            complement(7040..7258)
                     /locus_tag="VHV99_16455"
     CDS             complement(7040..7258)
                     /locus_tag="VHV99_16455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007583515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637042.1"
                     /translation="MMRSHLPSGTTHGLDFLIPDTWSADQALAVIELLDDLRERICTH
                     YQVALHELQREQRVPPVNAHPNDIDDPF"
     gene            complement(7255..8022)
                     /gene="istB"
                     /locus_tag="VHV99_16460"
     CDS             complement(7255..8022)
                     /gene="istB"
                     /locus_tag="VHV99_16460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEX3637043.1"
                     /translation="MNTPDELQIRANALRLYGLLAHWPDVAAEQWVAPLLQWEEDERG
                     RRSLERRIRDAHLGSFKPLCDFDWAWPKRCDRAAVEELMALEFVKDTANVVLIGPNGV
                     GKSTLALNLAYQAIVRGHTALFTTAGQMLGELAALDSDSALRRRLNRYASPDVLVIDE
                     VGYLSYSNRHADLLFELVSRRYGVASTVVTTNRPFAEWSEVFPNAACVVSLVDRLVHR
                     AEVIVIEGESYRVKEARERAEQRTKKRSAAKAEKKTS"
     gene            complement(8009..>8235)
                     /locus_tag="VHV99_16465"
     CDS             complement(8009..>8235)
                     /locus_tag="VHV99_16465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012354806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEX3637044.1"
                     /translation="AFELQAAIEETLASGAAPHPNPVRLALERRREARQAPPPVAIPL
                     PEHVRKKDTLVTPHRLDTYDQIAGDADEHA"
BASE COUNT         1658 a         2491 c         2485 g         1601 t
ORIGIN      
        1 cccgggcatg gcaaccacgt gcccagcaag gccgcctata ggtgtgcagt ctgtctcgcc
       61 gccgtcccgt cgttgtggtc cgactcgcaa tgcaggagca ttaacgaatg ccccactaaa
      121 cccaacttgc aaaaacgtgg aggtccttgt agatgggtgt ctggcgcgat ccggttcgca
      181 acctgttctg agaaaatgga tgcgataccg acctgcaggc agcggcgaca ggataacgaa
      241 ggaatgtcgc cggattgcca tgacgatgaa acaatgaaac tgaccatcgt cgtggcaatc
      301 cggcgacggg gaagattcgt accttgactt cggccgacta atcggtgtca cgctgctgtg
      361 atggtcgagt aagatcgcgt gatgaagaat gcaaaatcgc tctgtcacgg tcatcgttcc
      421 ccggcggcgg acataagttg ggcggtgcgc tgatatttca ggtttcagct cagcccgcgt
      481 gatatccaag agctgctgtt cgagcgcggt gttatcgtta gctacgaaac ggtccgacgt
      541 tgctgcgata agttcggtgc gtgctcgcac gccgtgcccg gcaccacatg gcgcctcagc
      601 acatgaggca agcacgcgct tgaaaaactg cttcgcacgc cacaacaggt aaggctcgtc
      661 gcgcaacctc acaaatactt cgccaaggtc ttcgttaaag cgagcgcgcg gatcaatgcc
      721 gcgccggaaa cagtcgtcag ccgtcgcgcg aacgcgagcg acgcatgcgc ggcttgcgcg
      781 atcctcaacg cacgccggct ttcttatcgc acttcggtcc gatccgccac cacttcgcgc
      841 tcaagcgaca cctgctacgc gcctcactct atcgcaaaca gctcgccgca cgcttcgccg
      901 cctggcatcg tttcactgag gccacccagg aaccgtccac tttctgagca agcgcgtgtc
      961 ctctccgcca ttgcgtcgcg aacaatttaa cgtgacagcg ccgtacaccg aactgatgct
     1021 tcgggctctt gacatggcac tgctgcagcg cggccctgac gggtgatcca tcactctgat
     1081 cagggttgcc accccacatt caactggtcg tcgcaacacc gagaaatcag ggagttgcaa
     1141 tgggaaccaa aaaaaatatg ggcgctgtcg gcgtgaagtg cgcccagcgg tgggttcggt
     1201 cgggcgaccc ggcgtcgatc tgagggaaaa ccgagagcga ttctggaagg cgattgccca
     1261 aggcttgcca agcgagacgg cagccgtggt agccggctta tcaccagcgg tgggagtgcg
     1321 ctggttcagg caaagtggtg gcatgcctaa tatcagccta accctgcttt ttcagcccgt
     1381 tttctctcct tctccgagcg ggaggagatc gcgcttctgc gcgcgcagga tcacggcgtt
     1441 tgtgaaatcg ccagaccgac cggccgacat gcctcgacga tctcgcgaga gctgcgccgc
     1501 aatgccgcca ctcgaagcga gttatgcaga gcatcgtgct tcgacggcgc aatggcacgc
     1561 acggaagcgt gccagacggc cgaagactgc catgctcgtc gccaataccc ccctctggca
     1621 ggtaacaggc cactggcaag gcgatccgat tattgggctg ggacgctccg caatcggaac
     1681 gctcgtagag cgcacgaccc gctttatcat gctgttgcac cttccccggc atggctgaac
     1741 acggaacggc tcgagagaaa aatggaccag cgctagccgg tctcggggct gacgcggtca
     1801 gagcggcaat cgctgccttg atgctgccgc tgccgccaca cttgtgtagg tcgctgacat
     1861 gggaccatgg gtgcaaaaag atggctcagc atacccaact gcggatcgac aagggcatgc
     1921 aaatctactt ctgcgagcct cgtagtccgt ggcaacgaca ccctcgccat caagcggcgc
     1981 ctgcaacgag cttcgcatta tcgcgcggga cttgccgcat ctattggatc aatgaagaca
     2041 agtcattggc agttgtgtgt cgcataccat agcatcttga tcagcccttc tgctgaccga
     2101 ctgtcctgac atgaacctgc tttcgattgg aatcaatcac cacaccgcgc cggttgcctt
     2161 gcgtgaacgc ctggcattcc cgcttgaaca gattcggccc gctctgcaga cgttgaaagc
     2221 cgcgtggctg cggcgcgata gcgcgcgttt cagcacagcg cataccccgg agccagttga
     2281 acggctagca tccagcggca atgcgctgtc cgaggcggct atcctctcaa cctgcaatcg
     2341 caccgaaatc tattgcgcga ccgatgatcg tgccgcgccc gagcacgcaa tagcatggct
     2401 gtccgcgtat cacggaattg ccgtcgacga gttcgtgccg catgtctaca cgctgccgca
     2461 atcggaggcg gtacgtcatg cgatgcgcgt cgcgtcgggc ctcgactcga tggctctcgg
     2521 tgaaacgcag attctcggcc agatgaaaaa cgcggtgcgc gccgcttctg aagccggcgc
     2581 gatgggcact tatctgaacc agatgttcca gcgcaccttc tcggtcgcca aggaggtgcg
     2641 cgaccagact gagattggcg cgcattcggt atcgatggca gcagccgcgg tgcggctggc
     2701 acagcggatt ttcgatgaaa tctcaagcca gcgcgtactg ttcatcggtg cgggcgagat
     2761 gatcgggctg tgcgccaccc attttgcggc gcgcaggccg cgcgagctgg tagtcgcgaa
     2821 ccgcacccct gagcgcggca gcgcgctcgc caaacgcgtg ggcggtcagg cgatcgcgct
     2881 tgttgatctg cccgagcggc tgcacgaatt cgacattgtg gtgtcgtgca ctgcgtcctc
     2941 gttgccgatc atcggcctcg gcgcagttga gcgcgcaatc aaggcgcgca aacaccggcc
     3001 gatgcttatg ctcgaccttg cggtgccgcg cgatatcgag caggaagtcg aacagctcgg
     3061 cgacgtctat ctgtacacgg tagacgatct cgccgagatc gtgcgcgaag gcgccgcgac
     3121 gcggcaggcc gcggtcgccc aggccgaagc gatcatcgag acacgcgttc aagggtttat
     3181 gcggtggctc gagcagcgca gcgtcgtgcc ggttattcgt catatgcata cgcaggccga
     3241 catgttgcga cgcgtcgagc tcgagcgcgc gcagaagatg ctcgcacgcg gcgacgaccc
     3301 cgccgtggtg ctcgaagcgc tttcgcaggc gctcaccaac aagttcattc acggtccgac
     3361 gaatgcgctc acccacgtcc acgcggacaa ccgggacaag cttgtcgacc tgttctctgg
     3421 cttttaccat cacccggggc cgtccggagg ttagggcaac gctcgctatc tcaatcgacc
     3481 gatcgaccaa ttgagcaaca aactcgcgcg gtacgtccgg gacgctgcgc cccgacttcg
     3541 ccgcctggat tttgagcctg gtgtgcttac cctgtgtcgc gaccgacctc cccttggtct
     3601 ggcattcaag ctaatccagg gagcagagaa aacctggcgc catatcaatg gccccgacaa
     3661 gatcaagtcg ctgctagatg gcctcgcctt cagggacggc gacccggtga aggacgatca
     3721 accggctcag cagaaagtcg ccgcgtggag tctctgttca tcaagtcgtc cctatacggc
     3781 tctttaatgt gtcagcccct tgacgttaag gtgacgtttt cgacatatat gcatgaaaaa
     3841 tacgaaatgc acaattgcga aaattgcaac tgtttgaaag gctagacttc gatctctcgc
     3901 ggacgcagcc ctgtttgcga gaaccataca gttacggaga gttttgatga agtacaaagt
     3961 attgtgtgcc acgatgctcg cagcaattgg cacgaccgca gcccacgcac aaagcagcgt
     4021 gaccctgtac ggcctgctgg atgcgggcat cagctacacc agcaacaccc gcgtcggcac
     4081 cacaggtcac agcaatgtcg ccctgagcaa cgccatgatg caaagcagcc gctggggcct
     4141 gcgaggctcc gaagatctgg gtggcggcct gaaggcagtg ttcgtcctgg aaaacggctt
     4201 cacgctgacc aacggcaagc ttggccaggg tggccgcgaa ttcggccgta acgcctatgt
     4261 cggcctgtcg agcaatgccc tcggcaccct gacggtgggt cgtcagaacg aattcatgta
     4321 cgactgggtt agccgtgtgt cgatggaaaa cttcaacggc gcaggcggca atgtcttcgc
     4381 gcacgcgctg gacaacgaca acatgctcgg tacgttccgc gtcaacaacg cggtgaaata
     4441 ccagagcgcg aactacgccg gcctgacctt tggcggcttg tttggtttct cgaaccaggc
     4501 cggcggcttc ggcaacaacc gtgcatacag tttcggcgcc atgtacaaca acggcccgat
     4561 tctggcgacg ttgggctata cgcaggtaaa caacagcctg accaacagca ctgcgggcct
     4621 gggcgtagtc accaacaccg gtggcgcggt tgatagcagc gccgccagca ccagcgctga
     4681 tgcggccctc actgcaggac gtcagcgcat cttcggcggc ggcctgaact acacgttcgg
     4741 cccggcgaaa gttggttttg tgatcacgga cagcaagctg gacaatgcaa ccgggttcac
     4801 cagtggcacc tcggctttgt cgcttgcgaa ctccgcatat gttcatttca cgaactacga
     4861 agtgaacgcg cattacaacc ttaccccggc attgaccctg ggcgcggctt acacctacac
     4921 cgatggcaag ttcagcaacg cccagaattc ggtatcgccg aagtggagcc aggtcagcct
     4981 gtcgtcggat tacaacctgt cgaagcgcac ggacgtgtac gcggtcgcga actggtcaca
     5041 cctgagcggt ggtctgtcgg cacaggccgg ttcgggcctg gctgcattcg aaggcgcaag
     5101 ctcctatatc ggtggcattg cggcgacgaa gaaccaggtg gtagtgggtg ccgggatgcg
     5161 cacccgcttc taagcactga gttgttgatg taaaaagggt accgttcgcg gtgccttttt
     5221 ttgttgggcg cgcggatgcg cccctcggcc cgcgcggtcc cggcaccgtg ctatttcacc
     5281 ttatgggcag cgttcgacac ttgaaggcac ctacgctgcc acgccgcaaa gcggcattca
     5341 ataatagggt ttatacttaa tcacctttca agatttgtct ccgaaatggt ttaagccccc
     5401 tccctgaggt gggctttttt ttcggcgtgt tatcggccgt cgctgccgtc caaaatgtgc
     5461 gcatcgtttc ttagaacgat cgcacctgcc gcagaaggta cagctaaaaa ggccgcacgg
     5521 cctgaagtgt cccgcactga aatgcagcgt cgcacgatgt tgtcctgctg gccctgacga
     5581 gagcaaaggc gaagagtgta tcggcccggt caccttctgc gacattttct ccgacggttg
     5641 gacgcaaacc ggcactcaag ctccgcgatc taatggcgct cactacaggc tagccgccag
     5701 catagatcgt cgagtacagg tatgcgaatt gcccgtcctt ttcggcatga accaattcct
     5761 gatagacctg tgcgcgtgtc ttgccggcca gaggttggcc agatggtgca accggctgtc
     5821 ctgctggcga agcgacgggt gtcgtattcg atgcaatctg aaccgctctg gtcgccggtc
     5881 gggggttgac cgataaggca gatggagtcg actgcgcaaa agcggccgtt gccgacaacg
     5941 ttccagcaaa agcaatagcg atcgtcatgt atttcatatc cggtgtcctc acctgtgcaa
     6001 agtaacgacc gatccctcta gcattaccgt ttgtgaatca gtcgataaag agcgtagtcg
     6061 cctgcgccta gcggatcggt tgccgaaagg tgaaatcgaa ttcatgcttc gcaaccagag
     6121 cggggaagaa cagggtgaag agcgcctcgc ttctgccctc gtcctccctg aacggacgcg
     6181 agatgtggtg tggtgcaaat agcgcaggcg aaccagaatc cgccctgctg tgcgaaatcg
     6241 ggctgcagca gcagccatcg acgctatacg cggatcggcc acctcgttca ggctgatctc
     6301 atcgtaccgg tcattacagc tgggtcatac gcgtcgatga gtggctcgcc cgccgcagcg
     6361 ttctccagcg tgccgccgtt tttcagaaag gcgccactgc ccgtggcacg gaacgtgtgg
     6421 ttgcgaatct tcgtgccgat gccggcgacg agcgcacagc gccgcaccat cgcgcatgcg
     6481 ttcgcctgcg gcagcggcgt cgtgccgagc tgcccgatgc agcgccggat cgtgcggaac
     6541 aggggcccct tcggatcgtc accgataccg gtgtgccctc gaagtaggcg tgcaggtacg
     6601 cttcgagcgt gtggtgacac ggcatctcgt gacgctcgcc gcctttctca cgcaggcgca
     6661 cgcccagccg ccgctgctgc tcataagaca tcctccaccc gcattgcgag cgccgcaccg
     6721 acgcgtgcga acgaaagcac cgaaagcacc atcggcgcaa tcagcgcgcg atcgcgcagg
     6781 ccagtcgggg tcgtcacatc aatgctgtag agcagctgcc gcgcctcggt cgcggcgagc
     6841 acgggcgtct tgcccttttt cgtggtgcga cctggaccgc accgacacgg ccgggttgtg
     6901 cggcggatcg ggccggtcac gcgccagtcg aacaggggac ggacgccgcg agttgttgcc
     6961 ggcggtgtac aagcgcggct agatggcggc aagggataaa agagaaacaa ccgaggccga
     7021 tgtcggcccg cggttggggt taaaagggat cgtcgatgtc gttcgggtga gcattcacgg
     7081 gagggacgcg ttgctctcgc tgcagttcat gcaacgcgac ctggtagtgc gtgcagatcc
     7141 gctcgcgtag atcgtcgagg agttcaatga cggcgagcgc ctggtcggcc gaccaggtgt
     7201 cggggatcag gaagtccagc ccgtgcgtgg ttccggaagg caggtgacta cgcatcatga
     7261 cgtcttcttt tcagctttag ctgcgctacg ttttttcgtg cgttgctcag cgcgttcacg
     7321 cgcctccttc acacgatagg actcgccctc gatcacgatg acctctgcac gatgcaccag
     7381 tcggtcaaca agcgagacga cgcaagcggc gttgggaaac acctcggacc attctgcaaa
     7441 cggtctattt gtagtaacca cggtcgaagc taccccgtac ctccggctga ccagttcgaa
     7501 cagcagatcg gcgtggcggt tcgagtacga gaggtagccg acttcgtcga ttaccaggac
     7561 gtcgggtgag gcatagcggt tcagtcggcg acgtagcgcg gaatcactgt cgagcgcggc
     7621 aagttcgcca agcatctggc cggccgtagt gaacaaagca gtgtggccac gtacgattgc
     7681 ctgataggcg agattgagcg ccagcgtcga tttgccgacg ccgttcgggc cgatcagcac
     7741 gacgttcgcg gtgtccttga cgaactccag cgccatcaac tcttcgacgg cggcacgatc
     7801 gcaccgtttg ggccaggccc agtcgaagtc gcacagcggt ttgaagctgc ccagatgggc
     7861 atcgcggata cgtcgctcca gagaacgacg accgcgctcg tcctcttccc attgcagcag
     7921 cggcgcgacc cactgctcgg ccgccacgtc gggccaatgc gccagcagtc catacaggcg
     7981 caaggcgttc gcgcgaattt gcagctcatc aggcgtgttc atcggcgtct cccgcgatct
     8041 ggtcgtaagt gtcgagtcgg tggggtgtta cgagcgtgtc tttcttgcgc acgtgttcgg
     8101 gcaatgggat ggcaacgggc ggaggtgcct ggcgcgcttc gcgccggcgc tccagcgcca
     8161 gacgtaccgg attcgggtga ggtgcagcac cactggcgag agtctcctca atggccgcct
     8221 gcagttcgaa ggcgc
//
LOCUS       DATASH010000604         6881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2078364,
            whole genome shotgun sequence.
ACCESSION   DATASH010000604 DATASH010000000
VERSION     DATASH010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6881
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2078364"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VHV99_16470"
     CDS             complement(<1..253)
                     /locus_tag="VHV99_16470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HEX3637045.1"
                     /translation="MNTPLATASGTTSVTPAVASAGGARPATASAPAGLPTLPRPQAD
                     EFDAIIRHGAYMPLTQFGVIDATGEEAASFLHGQLTNDTQ"
     gene            332..1342
                     /gene="mltG"
                     /locus_tag="VHV99_16475"
     CDS             332..1342
                     /gene="mltG"
                     /locus_tag="VHV99_16475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX3637046.1"
                     /translation="MSLLKKCFVAGSIVVVLAAAAVAGGYHWANSPLDLTPAQLDVTV
                     KPHSSLRSVTLQLNRGGVPVEPELFVIMTRLFGLQSDLKSGNYEFKTGITPYEVLQKI
                     ARGDVNEYVATIIEGWTFRRMRAELDANPALRHDTIGMSDADLMNAINAPEASIGNGE
                     GLFFPDTYLFDKNTSDLDIYRRAYRLMRLRLDEAWTARAPNLPYKTPYDALTMASIIE
                     KETGKRSDRPLVAAVFANRLRVGMPLQTDPTVIYGMGDSYTGHIRKKDLQTDTPYNTY
                     TRMGLPPTPISLPGVASLEAAMNPAQSTALYFVSRGDGSSIFSDTLGDHNKAVDKYIR
                     GQ"
     gene            1342..1962
                     /gene="tmk"
                     /locus_tag="VHV99_16480"
     CDS             1342..1962
                     /gene="tmk"
                     /locus_tag="VHV99_16480"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEX3637047.1"
                     /translation="MARGKFITFEGIDGAGKTTHLGWFRERLEQKVASTGRSVVMTRE
                     PGGTPLGEQIREIVLHQKMDLETEALLMFALRRQHLAEVIEPALARGDWVLSDRFTDA
                     TFAYQGGGRGLPRDKLETLERWVQGGFQPDLTVLFDLAPEIANERRSAARDPDRFESE
                     SEAFFNRTRAEYLRRAEEAPYRFAIIDSAQSIVRIQKQLEELITVL"
     gene            2057..3091
                     /locus_tag="VHV99_16485"
     CDS             2057..3091
                     /locus_tag="VHV99_16485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEX3637048.1"
                     /translation="MIYPWQADDWNRLQQLRGHWPHALLLLGQAGIGKLRFAQHLAQG
                     LLCEAQQPNGEPCGACVACNWFTQGNHPDYRIVVPEALAAEAGLANAADEKAEKAEGD
                     EGKKTRAPSKEIKIEQIRGLLDFVGVGSHRGGARVVVLYPAEGLNIAAANALLKTLEE
                     PPAGVVFLMVSARIDRLLPTIISRCRQWPMTMPAPQVATQWLAAQGVAEAPALLAEAG
                     GAPLTALALASDENRALRDWTLKQLGAGAQCDAFACGEALQKLPVPLVLGWLQRWMYD
                     LLAQRTAGAPRYFPQASAALTRCASQADASAFARFMRTVTRQRAVENHPLNARLVFEE
                     LFIGYRELFA"
     gene            3189..3707
                     /locus_tag="VHV99_16490"
     CDS             3189..3707
                     /locus_tag="VHV99_16490"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3637049.1"
                     /translation="MSLSYRDATLDDLPAIVAIYNSTVPSRLVTADLEPVSVESRLAW
                     FHAHGPQKRPLWVVDDAQQPGRVIAWLSFSDFYGRPAYQRTAEVSIYLDESARGKGLG
                     KQLLAASLKAAPALGIDTVLGFIFGHNDASLRLFRGFGFDTWGSLPRVAVLDGVERDL
                     VILGKRLDAAAA"
     gene            3837..4628
                     /locus_tag="VHV99_16495"
     CDS             3837..4628
                     /locus_tag="VHV99_16495"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX3637050.1"
                     /translation="MFVDSHCHINFEGLADRLPQVLENMRSHSVTHALCVSVDLETLP
                     SVLEIASQHENVYASVGVHPDHEDMREPSLAELIELAEHPKVVAIGETGLDYYRLEGR
                     TIADMEWQRERFRTHIRAAHATQKPLIIHTRSSSEDTLRIMAEERASEPGGVMHCFTE
                     PWTVAERALAQNFYISLSGIVTFKSATEVQDVARRVPLDRLLIETDSPYLAPVPYRGK
                     PNEPAYVSYVGRFIAQQRDMPDTALAAATTQNFFRLFKIPAPAGA"
     gene            4681..5421
                     /locus_tag="VHV99_16500"
     CDS             4681..5421
                     /locus_tag="VHV99_16500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX3637051.1"
                     /translation="MTNHSTLTTAFQEASTVQIALASARRRVSRAALAATLACAALTA
                     LVASPVHAAPIDSLIKSVKFDDIDGVNKLLAKGMDPNSVDDQGMPLLVIAAREKSNKV
                     GAALVANPKTNIEIQDKAGENAMMMAALNGDLDFVNLLIAKDAEVNKKGWAPLHYAAA
                     NGHDDIVKVLLDHSAYVDAGSPNGTTPLMMAARGGHVSTVKLLLDNGADLTVKNQIGL
                     TALDFAKTYKEPDVVEGLTARLQQMQQK"
     gene            5612..5968
                     /locus_tag="VHV99_16505"
     CDS             5612..5968
                     /locus_tag="VHV99_16505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rap1a/Tai family immunity protein"
                     /protein_id="HEX3637052.1"
                     /translation="MLRALIFTSALAVPLSAAAFTGGDLNKLCTKTDVASRSACAAYI
                     EGAADGIFNTIDAIGGTTGPRVGQYFCLPADARSAQLTDAVRKYIAENPIVAGYNAST
                     AISLGLGKAFPCRSGS"
     gene            6442..>6881
                     /locus_tag="VHV99_16510"
     CDS             6442..>6881
                     /locus_tag="VHV99_16510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEX3637053.1"
                     /translation="MGRLIVVSNRVATPTETKGSAGGLAVGVFGALKDAGGVWFGWSG
                     DVVSETVANAGPTLEQDGAVTFATVGLTRKDYDQYYRGFSNATLWPVFHYRNDLARYE
                     REEYAGYRRVNAWLAHKLIKLLEPDDVIWIHDYHLIPFAEALRAA"
BASE COUNT         1345 a         2188 c         2136 g         1212 t
ORIGIN      
        1 gctgggtgtc gttggtcaac tggccgtgca ggaagctcgc cgcctcctca ccggtggcgt
       61 cgattacgcc aaactgcgtc agcggcatgt aggcgccgtg tcggatgatg gcgtcgaact
      121 catcggcttg cgggcgcggt agcgtgggaa gtccggcagg ggcggaagcc gttgccggcc
      181 gagcgccgcc cgcggaagcg actgcgggcg ttactgaagt cgtgcctgaa gcggtagcga
      241 gcggtgtgtt catggattcc gagaacagtc aattctgact ttagctaagg caaacaagta
      301 ttatatgagg tccaacccag ccacgtttcg catgtccctt ctgaagaaat gtttcgtcgc
      361 cggctcaatc gtcgttgtgc tggccgcagc cgccgttgca ggcggatatc actgggccaa
      421 cagcccgctc gacttgaccc ccgcgcaact cgacgtcacc gtcaaaccgc acagcagctt
      481 gcgcagcgtc acgctgcagc tcaaccgcgg cggtgtgccg gtcgagcctg agctgttcgt
      541 tatcatgacg cggctgttcg ggctgcaaag cgatctgaaa tccggcaact acgagttcaa
      601 gacgggcatc acgccgtatg aagtgctgca aaaaatcgcg cgcggcgacg tcaacgaata
      661 cgtggcgacc atcatcgaag gctggacctt caggcgcatg cgcgctgaac tggacgccaa
      721 tccggcgctc aggcacgaca ccatcggcat gagcgacgcc gatctgatga acgcgatcaa
      781 cgcaccggaa gcgtcgatcg gcaatggcga ggggctgttt ttcccggaca cctatctgtt
      841 cgacaagaac accagcgatc tggacattta ccgccgggcg taccggttga tgcggctgcg
      901 tctggacgaa gcgtggaccg cgcgtgcgcc gaacctgccg tacaagaccc cgtacgacgc
      961 cttgaccatg gcgtcgatca tcgaaaagga aaccggcaag cgatccgacc ggccgctggt
     1021 ggcggccgtg ttcgcgaacc gtctgcgggt gggcatgccg ctgcaaaccg acccgaccgt
     1081 gatctacggc atgggcgaca gctacaccgg ccacatcagg aaaaaagacc tgcagaccga
     1141 cactccttac aatacgtaca cccggatggg gcttccgcca acacccatct cactgcccgg
     1201 cgtcgcctcg ctggaggcgg ccatgaaccc ggcgcaatcc accgcgctgt atttcgtatc
     1261 gcgcggcgac ggcagcagca tcttttctga caccctcggc gatcacaaca aggccgtgga
     1321 caaatacatt cgagggcaat gatggcgcgg ggcaaattta taacgtttga gggcatcgac
     1381 ggtgccggca aaaccaccca cctgggctgg tttcgcgaac gcctcgaaca gaaagtcgcg
     1441 agcaccggcc gctcggtcgt gatgacgcgc gaaccgggcg gcacgccgct tggcgagcag
     1501 attcgcgaga tcgtgctgca tcagaagatg gacctcgaaa ccgaggcgct gctgatgttc
     1561 gcactgcgcc gccagcatct ggccgaagtg atcgaaccgg cgctggcacg cggtgactgg
     1621 gttctgtccg accgtttcac cgacgcaacc ttcgcctacc agggcggcgg ccggggcctg
     1681 ccgcgcgaca agctcgagac gctggagcgc tgggtgcagg gcggttttca gccggacctg
     1741 acggtgctgt tcgacctggc gccggaaatc gccaatgaac ggcgcagcgc cgcgcgcgat
     1801 ccggatcgtt tcgaaagcga gtcggaggca tttttcaacc gcacgcgtgc cgaatatctg
     1861 cgccgcgcgg aagaggcgcc gtaccggttc gctatcattg actccgcgca gagcatcgtc
     1921 cgtattcaga agcaacttga agaattgatc acagttcttt gatttcaaaa gaacataatc
     1981 cgaaattata aaggtaaacg aagttcgctt gatgtctcat caagcgctgt taacccactg
     2041 atttaaaaga gaaacaatga tttatccgtg gcaagccgat gactggaacc gcctgcagca
     2101 gttgcgcggg cactggccgc atgccttgct gctgctgggc caggcaggga tcggcaagct
     2161 gcgcttcgcg cagcacctgg cgcaagggct cctgtgcgag gcccagcagc ccaacggcga
     2221 gccctgcggc gcgtgcgtgg cgtgcaactg gttcacgcag ggcaatcatc cggactatcg
     2281 gatcgtcgtg cctgaagcac tggccgccga agccggtctc gctaacgccg cggacgaaaa
     2341 agccgagaag gccgaaggcg acgaaggaaa gaaaacccgc gcgcccagca aggaaatcaa
     2401 gatcgagcag attcgcggct tgctcgattt cgtcggcgtc ggttcgcacc ggggcggcgc
     2461 gcgcgtggtc gtgctgtatc cggccgaggg gctgaatatc gccgcggcca acgcgctcct
     2521 gaaaacgctc gaagaaccgc cggcgggcgt ggtgtttctg atggtgtcgg cgcgcatcga
     2581 ccggctgttg cccaccatca tcagccgctg ccgccaatgg ccgatgacga tgcccgcgcc
     2641 gcaagtcgct acccagtggc tcgccgcgca aggcgtcgcc gaagcgcccg cgctgctcgc
     2701 cgaggcaggc ggcgcgccgc tcacggctct cgcgctggcg agcgacgaaa accgcgcgct
     2761 gcgtgactgg acgctcaagc aactgggcgc cggcgcccag tgcgacgcct tcgcttgcgg
     2821 cgaggcgctg cagaagctgc cggtaccact cgtgctcggc tggctgcaac gctggatgta
     2881 cgatttgctg gcgcagcgca cagccggcgc gccgcgctat tttccgcagg cgtcggcggc
     2941 tttgacgcgc tgtgcatcgc aagccgacgc cagcgcgttc gcgcgcttca tgcgcaccgt
     3001 gacgcgccag cgcgctgtcg agaaccatcc gctgaacgca cggctagtgt tcgaagaact
     3061 ctttatcggc tatcgcgagc tgttcgcata agtctgttga tggcggcctg ggcaggcccg
     3121 ctttaccgcg ccgcgcccgc ccatgcatag gtccgctgat tcgtcttcca tttcacgttc
     3181 aaagcaccat gagcctttcc taccgcgatg ccacgctcga cgatctgccc gcgatcgtcg
     3241 ccatttacaa ctcgaccgtg ccgtcgcggc tcgtcactgc cgatcttgag ccggtgagcg
     3301 tcgaaagccg tctggcgtgg tttcacgcgc acggcccgca aaagcgtccg ctctgggtgg
     3361 ttgacgatgc gcagcagccc ggccgtgtga tcgcatggct gagtttctcc gacttctacg
     3421 gccgcccggc ttatcagcgc accgccgaag tcagcattta tctggacgaa agcgcgcgcg
     3481 gcaagggtct cggcaagcaa ttgctggcgg cttcgcttaa ggcggcgccg gcgctcggca
     3541 tcgacacggt gctggggttt atcttcggcc acaatgatgc aagcctgcgc ctgttccgcg
     3601 gcttcggttt cgacacgtgg gggtcgctcc cgcgcgttgc ggtgctggac ggggtggagc
     3661 gcgatctggt gattctcggc aagcggctcg acgcggccgc cgcctgagtc atagccggat
     3721 tcacggcgag gtcgcggcct gatgcacgac cggatttacc accaggttcg cggccggctg
     3781 tacgaccaga ctcacggccg gattcacccc cagctattta tcagcaggac atcatcatgt
     3841 ttgtcgattc gcactgccat atcaacttcg aaggactcgc cgaccgcctg ccgcaggtgc
     3901 tcgagaacat gcgcagccat tcggtcacgc atgcgctgtg cgtctcggtc gatctggaga
     3961 cgctgccctc ggtgctcgaa atcgccagcc agcacgagaa cgtctacgca tcggttggcg
     4021 tgcatccgga tcacgaggac atgcgcgagc cgagcctcgc tgaactgatc gaactggccg
     4081 agcatccaaa ggtggtcgcg atcggcgaga ccggcctcga ctactaccgc ctcgaaggcc
     4141 gcacgatcgc cgatatggaa tggcagcgcg agcgctttcg cacccatatc cgcgcggcgc
     4201 atgccacgca gaagccgctg attatccata cgcgttcgtc gtcggaagat acgctgcgta
     4261 tcatggccga ggagcgcgcg agcgagccgg gcggcgtgat gcactgcttc accgagccgt
     4321 ggacggtcgc cgagcgggcg ctggcgcaga atttttacat ttcgctgtcg ggcatcgtca
     4381 cgttcaagag tgcgaccgag gtgcaggacg tggcacgccg cgtgccgctc gaccgtttac
     4441 tgatcgaaac cgattcgccg tatctcgcgc ctgtgccgta tcgcggcaag cctaatgaac
     4501 ctgcgtacgt aagttatgtc ggacgcttca tcgcacaaca acgcgacatg ccggatacgg
     4561 cgcttgccgc cgcgaccaca caaaacttct tccggctctt caagattccc gcgccggctg
     4621 gtgcgtgaca gataaagccg ataaatatca aatggatagg gaagtttcct aaagcaaaat
     4681 atgacgaatc attcgacttt gaccacagcg tttcaagagg cttcaaccgt gcaaatcgcc
     4741 ctcgcttcgg cacgtcgccg cgtgtcgcgc gcggcgctgg ccgccacgct cgcctgcgcc
     4801 gcgctgaccg cgctggtcgc ttcgccggtt cacgccgcgc cgatcgactc gcttatcaag
     4861 tcggtcaaat tcgacgacat cgacggcgtc aacaagctgc tcgccaaggg catggacccg
     4921 aacagcgtcg acgaccaggg catgccgctg ctggtgatcg ccgcgcgcga gaagtcgaac
     4981 aaggtgggcg ccgcgctggt cgccaatccg aagaccaaca tcgagattca ggacaaggct
     5041 ggcgagaacg cgatgatgat ggccgcgttg aacggcgacc tcgatttcgt caacctgctg
     5101 atcgcgaagg acgccgaggt caacaagaag ggctgggcgc cattgcacta cgcggccgcg
     5161 aacggtcacg acgatatcgt caaggtcctg ctcgaccatt cggcctacgt cgacgccggc
     5221 tcgcccaacg gcaccacgcc actgatgatg gccgcgcgtg gcggtcatgt gtcgacggtg
     5281 aagcttctgc tcgacaacgg cgcggacctg acggtgaaga accagatcgg cctgacggcg
     5341 ctcgatttcg cgaagaccta caaggagccg gacgtggtcg aaggactcac cgcgcgcctg
     5401 cagcagatgc agcagaagtg agtggtgcga agttgaccgt ggaattaccg gccgccagtc
     5461 ccacgaatta cacaaaattg cctgaagata gcgattgcag tcctgcagcc gcttcgcaaa
     5521 acagtgcaga atggcgactc ggcgtgcctc atggcgcgcc gtttcgatgc ggcgcaagat
     5581 cgcctgataa acgaccctgg aaaggaacac catgctgcgg gctttgattt ttaccagcgc
     5641 gcttgccgta ccgctatcgg cagccgcgtt tacgggaggc gacctcaaca agctgtgcac
     5701 gaagaccgat gtggcatcgc gtagtgcgtg tgccgcatac atcgaaggcg ccgccgacgg
     5761 catcttcaac acgatcgacg cgatcggcgg taccaccggg ccgcgcgtcg gccagtactt
     5821 ctgcctgccg gcggacgcgc gttcggcgca actcaccgac gcggtacgca aatacatcgc
     5881 ggagaatccg atcgtcgcgg gctacaacgc cagcacggcg atctcgctgg gcctcggcaa
     5941 ggcgttccct tgcaggagcg gtagttgatc gcgtagttca agtttgacgt gcatcgcgac
     6001 cgacgcctgc gccgcgtgct cgcatcgggc acgtcaggcg tttcagccgc gctgaaattt
     6061 tgctgccacg cgaattcaag ctcggccgcc cctccagggc gctttgccgc ctgttggccc
     6121 gacggccccg gtttgcgcaa taccccctta gcgtcacgcc cacgccacgt cataccgccg
     6181 ccgtcacgca cggatcgacg cgctgccgcc gttgacatac gccgcttgtg catggatcca
     6241 gacgatcatc gaaacaccga tgagtgcaac tgatcgcgca ccaaagcggt cgaaccgtcc
     6301 atctgactca cgaacctggc gcggtttcat actttcatac ggttcatttg cctgacgtca
     6361 tcgaagcgta gcggcgctcg ggacatgccc ggcatctgaa gcgcaaagac cgcgctgtgc
     6421 gaaccaggga ggactaccaa catgggccga ctgatcgtcg tgtcgaatcg cgtcgcaacg
     6481 ccaacggaaa ccaaaggatc agcaggcggc ctcgcggtcg gcgtgttcgg cgcgctaaag
     6541 gacgcgggag gcgtgtggtt cggctggagc ggcgacgtcg tgagcgagac cgtcgccaat
     6601 gccggcccaa cgctggagca ggacggcgcg gtgaccttcg cgaccgtcgg cctcacgcgc
     6661 aaggattacg accagtacta ccggggcttt tcgaacgcga cgctgtggcc ggtgttccac
     6721 taccgcaacg acctcgcgcg ctacgagcgc gaagaatacg caggctaccg gcgcgtcaac
     6781 gcatggctcg cgcacaagct gatcaagctg ctcgaacccg acgacgtgat ctggattcac
     6841 gactaccatc tgatcccgtt cgccgaagcg ctgcgcgccg c
//
LOCUS       DATASH010000605         2088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6033501,
            whole genome shotgun sequence.
ACCESSION   DATASH010000605 DATASH010000000
VERSION     DATASH010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6033501"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VHV99_16515"
     CDS             <1..529
                     /locus_tag="VHV99_16515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3637054.1"
                     /translation="LQQFMDYAGSGEREAAEPGDLNALIERLAGDFAGLGHEFALSLA
                     VLPAIAYRPISMMRLLMNLMQNAIVYGGAGLAVRSWAASDAVYVAVGDRGKGLSAEEL
                     EQLKAPFQRGRNARAHSGGTGLGLAIVERITRLHGGSLQFHAREGGGLEVWVVLPLIS
                     VPATHPAPRGTPRPR"
     gene            complement(474..740)
                     /locus_tag="VHV99_16520"
     CDS             complement(474..740)
                     /locus_tag="VHV99_16520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637055.1"
                     /translation="MPAQHDEVPTASAKPGQAKRKELSIVARNLMMVAVGVVVFAIVA
                     TIVLYGPITYGDQIPNFGIILMLTVPVIAGVAFRVGLDAWLERK"
     gene            complement(896..1243)
                     /locus_tag="VHV99_16525"
     CDS             complement(896..1243)
                     /locus_tag="VHV99_16525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003808952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1634 domain-containing protein"
                     /protein_id="HEX3637056.1"
                     /translation="MSRIASTGARLERWLARLLHYGTWVATVTIASGLAISLPSHSES
                     ALPGPRTHPEWLSTPTGLHIVTVGIALFILLPIMRLILMLGMFLHQRDYRFSAITALV
                     LAIVAAGLLVGAA"
     gene            complement(1240..>2088)
                     /locus_tag="VHV99_16530"
     CDS             complement(1240..>2088)
                     /locus_tag="VHV99_16530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX3637057.1"
                     /translation="WLFAVSLGASMLGGMLGMASGIFIVPILTMFGHLDIHLAIGASI
                     VSVIACSCGGAAPFLRGRLTNVRLAVVLETATTLGALSGVLLSGLVPVPALFFIFAAI
                     LLLSARQMLARRADPIAGHDAGAASAYAWGTALRLNSSYPDRALGREVDYRVQRVPLA
                     LSLMYGAGLISALLGIGSGVLKIPAMDTALRLPIKVSSATSNFMIGVTATASAGAYFL
                     RGEIVTAIAGPVALGSVVGAVLGARILMRVSNDRLRVLFVVALVLLAVQMLLEAFGIH
                     LAGAAA"
BASE COUNT          429 a          649 c          689 g          321 t
ORIGIN      
        1 cttgcagcag ttcatggact acgcgggcag cggcgagcgt gaggcagcgg agcctggcga
       61 tctgaatgcc ttgatcgaac ggcttgccgg cgatttcgcg ggactcgggc atgaattcgc
      121 gctgtcgctc gctgtattgc ccgcgattgc gtatcggccg atcagcatga tgcggctcct
      181 gatgaacctg atgcagaacg cgatcgtcta cggcggcgcg gggctcgctg tacgaagttg
      241 ggccgcaagc gacgccgtgt atgtcgcggt cggcgatcgc ggtaaagggc tttccgcaga
      301 ggagttggag cagctgaaag cgccgtttca gcgcggccgc aatgcccgcg cgcatagcgg
      361 tggaaccggg ctggggctgg cgattgtcga acggatcacg cggctgcacg gcggcagcct
      421 gcagtttcat gcgcgcgaag gcggcggcct ggaagtgtgg gtggtgttgc cgctcatttc
      481 cgttccagcc acgcatccag ccccacgcgg aacgccacgc ccgcgataac cggcacggtc
      541 agcatgagaa tgatgccgaa gttgggaatc tggtcgccgt acgtgatcgg accgtagaga
      601 acgattgtcg caacgatcgc gaagaccacg acgccgaccg caaccatcat cagattgcgc
      661 gccacaatgg acagctcctt gcgcttggct tggcccggct tggcgctggc agtggggact
      721 tcgtcgtgtt gggcgggcat gatggttctc gtgtctggaa gaagtgccgc gcaacgcggc
      781 tgcaaatgct acttcaaagg caatgacaac atgcgttacc aggcgcgcgg cggtgaatcg
      841 aacgccgact cgggcacgac cggcatcata cccggggtgg ttcgtgccgc acacatcaag
      901 ctgcaccgac cagcaacccc gcggcgacga tcgccagcac gagggcggta atcgcgctga
      961 accggtagtc gcgctgatgc aggaacatcc ctaacatcaa tataagccgc attatcggca
     1021 acaggatgaa aagcgcgatg ccaacggtca cgatatgcag gcctgtcggc gttgaaagcc
     1081 attcggggtg agtccgcggg ccgggtagtg cgctttcaga atggcttggc aggctaatcg
     1141 caaggccgct cgcaatcgtg accgttgcca cccatgtgcc gtaatgcagg agccgcgcaa
     1201 gccagcgttc gagccgcgcg ccggtcgacg cgatgcggct catgctgccg ctccggcaag
     1261 gtgaatgccg aatgcctcga gcagcatttg cacggcgagc aggacgagcg ctacgacgaa
     1321 gagcacgcgc agcctgtcat tcgatacgcg catgaggatg cgcgccccaa gcaccgcgcc
     1381 gacgaccgat cccaatgcca ccggcccggc gatggcggtc acgatctcac cgcgcaggaa
     1441 gtacgcgccg gcgctggccg tcgccgtcac gccgatcatg aaattggacg tggccgacga
     1501 gaccttgatc ggcaagcgca gcgccgtatc catcgccgga atcttgagca cgccgctgcc
     1561 gatgccgagc agcgccgaaa tcaagcctgc tccatacatc agggacaagg ccaagggcac
     1621 gcgctgcacg cgatagtcga cctcgcggcc gagcgcgcga tccggataac tcgaattcag
     1681 acgcaacgcc gtgccccacg catacgcgct ggctgcgccg gcgtcatggc cggcgatcgg
     1741 atcggcgcgc cgtgcgagca tttgccgggc ggacagcaac agaatggccg cgaaaatgaa
     1801 aaacagtgcg ggcaccggaa cgagtccgct cagcagcaca ccggacagtg cgccgagcgt
     1861 ggtggcggtt tccagaacca cggcgaggcg aacgttggtc aaacgcccgc gcaggaacgg
     1921 cgccgcgccg ccgcacgaac aggcgatcac cgaaacgatg ctggcgccga tcgccaggtg
     1981 aatatccagg tgaccgaaca tggtcaggat gggcacgata aaaatgccgc ttgccatgcc
     2041 gagcatgccg cccagcatgc ttgcgccgag cgacaccgcg aacagcca
//
LOCUS       DATASH010000606         2200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1287513,
            whole genome shotgun sequence.
ACCESSION   DATASH010000606 DATASH010000000
VERSION     DATASH010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1287513"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VHV99_16535"
     CDS             <1..280
                     /locus_tag="VHV99_16535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637058.1"
                     /translation="VSAAQAHVSVGIGIGVPAYPAYPVYAAPPPPVYYAPGPVYAAPP
                     VVYAPPPVVYGGYYGGGPYYRGHYRGGYGYGYHGGYRGYHGGHGGWRH"
     gene            503..1468
                     /locus_tag="VHV99_16540"
     CDS             503..1468
                     /locus_tag="VHV99_16540"
                     /EC_number="4.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threo-3-hydroxy-L-aspartate ammonia-lyase"
                     /protein_id="HEX3637059.1"
                     /translation="MSILPAPTYADVADAAERIAGAAHRTPVMTSRTADALTGANLFF
                     KCENFQRMGAFKFRGAFNAISHFSESQRRAGVLTYSSGNHAQAIALSARIAGIKATII
                     MPEDAPAAKMEATRGYGGEVITYDRYTENREEIGARLAQERGMTLIPPYDHPHVIAGQ
                     GTSAKELIEETGPLDYLFVCLGGGGLLAGCALAAAELSPSCTVIGVEPEAGNDAQQSL
                     ERGEIVHIEVPRTIADGAASTHVGEYNFPILKRYVDRVVTVSDDQLVQTMKFFAQRMK
                     MVVEPTGCLAAAAVLQNIVPVTGKRVGVIVSGGNVDLARLAQFLA"
     gene            complement(1479..2156)
                     /locus_tag="VHV99_16545"
     CDS             complement(1479..2156)
                     /locus_tag="VHV99_16545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEX3637060.1"
                     /translation="MHVLPLLSDGFFLSLSLCLDIGIANVAIISLTLSHGFRPGMMLG
                     LGTCVGDLVYAALALAGMAALLQFDTVRWVVWIGGAIVLLFLTFKMAREALFPASAPP
                     VAGEPDLVPAPRPSHLRSFLRGTLLAVSSPSAILWFAAVGGALIAKAGATGPASASVF
                     LTGFFCGGLAWTLFICSLASHGRKRAGTALLRACHVLSAVLFAYFAYSVIVNGYHDLI
                     LKGAQTL"
BASE COUNT          426 a          661 c          708 g          405 t
ORIGIN      
        1 tgtcagcgcc gcgcaggcgc acgtttcggt aggtattggc attggcgtgc cggcttatcc
       61 ggcttatccg gtgtatgccg ctccgccgcc gcccgtgtat tacgcgccgg gccctgtgta
      121 tgccgcaccg ccagtggttt atgcaccgcc gcccgttgtg tatggcgggt actacggcgg
      181 tggtccgtac tatcgcggtc attatcgcgg cggttacggg tacggatacc atggcggata
      241 tcgcggctac cacggtggtc atggcggctg gcgtcattga gatttcgggt atgaccggat
      301 tggcttgctg atcattggac tggctgcaaa acagcgcaac gctggcgtag agccaggttg
      361 cgctgttttt ttgcaccggg ctctccaacc gcgctaatcg cggcacggat gaagcctgcc
      421 gtgttggcta tttgaccagc tttccaaacg ctccacgatg ctgggacaat agccgttcct
      481 caaccgccgc ctctgagtca cgatgtcaat tcttcccgca cccacctacg ccgacgtcgc
      541 cgacgccgct gaacgtatcg ctggcgccgc gcatcgcacg cccgtgatga cttcccgcac
      601 cgccgacgcg ctcactggcg cgaacctgtt cttcaagtgc gagaatttcc agcgcatggg
      661 cgctttcaaa tttcggggcg cgttcaacgc gatctcgcat ttctccgagt cccagcgccg
      721 ggccggcgtc ctgacttatt catccgggaa tcacgcccag gcaatcgcgt tgtcggcccg
      781 tatcgccggc atcaaggcaa caatcatcat gccggaggac gcaccggccg cgaagatgga
      841 ggccacgcgg ggttacggcg gagaagtgat cacgtacgac cggtacaccg agaatcgcga
      901 ggagattggc gcaaggctcg cgcaagagcg cggcatgacg ctgattccgc cctacgacca
      961 tccgcacgtg attgcggggc aggggacatc ggcgaaagaa ctcatcgaag aaaccgggcc
     1021 gctggattat ctgttcgtgt gcctgggtgg cggaggcttg ctcgccggat gcgcgctggc
     1081 ggcggccgag ctgagcccgt catgcacggt gatcggtgtt gagccggagg cgggcaacga
     1141 cgcgcagcag tcactcgaac gcggcgagat cgtccatata gaagtgccgc gcacgattgc
     1201 cgatggcgcc gcgtccacgc acgtgggcga atacaacttt cccattctga agcgctacgt
     1261 ggaccgggtc gtcaccgtca gcgacgacca gctcgtgcag accatgaagt tcttcgcgca
     1321 gcgcatgaag atggtggtcg agccaacggg ttgtctggct gcagccgccg tgctgcagaa
     1381 catcgtgccg gtgacgggca agcgcgtggg cgtgattgtc agcggcggca atgtcgacct
     1441 ggcgcggctc gctcaattcc tggcttgatg ccctgaggtc ataacgtctg tgcgcccttc
     1501 aggatcaggt cgtgatagcc gttgacgatc acgctgtagg caaagtacgc gaacagtacc
     1561 gccgataaaa cgtggcaagc gcgcagcagc gccgttcctg cgcgtttgcg accgtggctg
     1621 gcgagcgagc agatgaagag cgtccaggcg agcccgccgc agaagaagcc agtgagaaac
     1681 accgacgcgc tcgccgggcc ggttgcgccg gctttcgcga tcagtgcgcc gcccacggcg
     1741 gcgaaccaga ggatggcgct gggtgacgag actgcgagca aggtgccgcg caagaagctg
     1801 cgcagatgag agggccgtgg tgcaggcaca agatccggtt cccccgcaac cggcggtgct
     1861 gatgccggaa agagcgcttc gcgcgccatt ttgaaggtca ggaagagcag cacgattgcg
     1921 ccgcctatcc atacgaccca tcgcacggta tcgaactgga gtagtgcagc catgccggcg
     1981 agtgcgagcg ccgcgtagac gagatcgccg acacaggtgc cgaggccgag catcatgccc
     2041 ggcctgaagc cgtgggaaag cgtcagtgag atgatcgcga cgtttgcgat accaatatcc
     2101 agacagagcg acaacgacag aaaaaatcca tccgacagca atggcaacac gtgcatccgg
     2161 actttccttt ttgatctttt tatattgagc gaagccggtt
//
LOCUS       DATASH010000607         6813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_99776,
            whole genome shotgun sequence.
ACCESSION   DATASH010000607 DATASH010000000
VERSION     DATASH010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6813
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99776"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..397)
                     /locus_tag="VHV99_16550"
     CDS             complement(92..397)
                     /locus_tag="VHV99_16550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2917 domain-containing protein"
                     /protein_id="HEX3637061.1"
                     /translation="MREVRIFELEHGEPAAAWRVARPSIFKVISGEIWLTVEGDNMDH
                     WLATGQSTELPRGTVAWISAGQAGARFALASGSERAATRPLRGSPILNWLPRWLGVA"
     gene            786..2204
                     /locus_tag="VHV99_16555"
     CDS             786..2204
                     /locus_tag="VHV99_16555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX3637062.1"
                     /translation="MKLEIQLDRDNGVPLTEQIVTGVTAWIRSRTAHPGAKLPSIRQF
                     AADYGVSRFPVIEAYDRLVSLGYVDSRHGSGFYVADRLPASTQCQGTSDPRRAEDESG
                     HILQQFNHPGETLKLGSGFIPDAWRDMDSLAQAIRHVSRTDTASMVDYATPLGNLTLR
                     EHLQGRIGQLGIQADASQILITNGASQALDLLMRYMLKAGDTMFVEDPGYYNLYGLLK
                     LHGVKLIGIPRTRNGPDLDVLQAQLQLHRPKLMFINTVFHNPTGTTVAPPVAFRLLQL
                     AREHGFAIIEDDIYADFQTDVTDRLATLDQLERVIYVGGLSKTLSSSLRIGCVVANHA
                     IIKDLIDIKMLTSLGASRFAEAVAVSLLERGAYRKYLERLRRRMRDALGSTIQTLEDA
                     GWEVFEKPVGGKFVWARVPHVDNAERLVACGAPLGVTVLPGSYFRPNMEVSPWIRIHA
                     AFGNEPRAQAFFLAAAALPASG"
     gene            2527..3987
                     /gene="mdtD"
                     /locus_tag="VHV99_16560"
     CDS             2527..3987
                     /gene="mdtD"
                     /locus_tag="VHV99_16560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HEX3637063.1"
                     /translation="MSTPPTLPAAAAAAVPTPSARSLTVMLWLVATGFFMQTLDSTIV
                     NTALPAMATSLGELPLRMQSVVIAYSLTMAVMIPVSGWLADKLGTRRVFFSAILVFGL
                     GSLLCANAHSLNQLVIYRIMQGVGGAMLLPVGRLAVLRTVPAERYLSALSFVAIPGLI
                     GPLIGPTLGGWLVKIASWHWIFLINVPVGIAGCIATFIFMPDSRNEHTGKFDLKGYLL
                     LIVGMVTISFALDGRTEFGIQHATVLVLLILSLASFVAYGLHAVREPAPIFPLDLFKI
                     HTFSVGLLGNLFARIGSGAMPYLIPLLLQVSLGYSAFEAGMMMLPVAAAGMVAKRVVT
                     TLIVKYSYRRVLIINTALVGLTMASFALTSAGQPLWLRLVQLAFFGGVNSMQFTAMNT
                     LTLKDLGTGGASSGNSLFSLVQMLSMSLGVTVAGALLATFTGLLPRVTAANSLPAFHA
                     TFLCVGIITAGTSWIFAQLSPDIRTLGTKTDPSERT"
     gene            4421..5695
                     /locus_tag="VHV99_16565"
     CDS             4421..5695
                     /locus_tag="VHV99_16565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX3637064.1"
                     /translation="MSMIELDPPGFQPPRPMAGDEGSRRTVLYGGYTVFSVFQPVFSV
                     SHRRAIGYHASLRAHDEHALQVPSHEVFTQAARRGDLLELGRLAESLHLGNFNAFDSH
                     DEWLFLSLHPAALMDTSYGDALLAGLKALGLPPQRVVLEVSEQAGGETTRFAEIVDAL
                     RKSGFLIALDGFGAKHSNIDRVWNLRPDIVTLDRCILAQASEHSHIERVLPGLVSLLH
                     ESGQLVLMGGLSTERDALIALECNVDFVQGAFFAGPSVEPVKPQAAAGLMDSLSAALR
                     ERVATRERVQSARLAPYVTALETAAAQLVAGESVTQATAPLLTLGETARCFVLDGSGR
                     QIGDNVLPPGRASQRAKRFRPLLHSEGASWERRPYFIQAMRMPGRVHLTAPYLSINEA
                     HLCVTASIAAHTPHGTQVLCVDINWEVATHRN"
     gene            complement(5651..6625)
                     /locus_tag="VHV99_16570"
     CDS             complement(5651..6625)
                     /locus_tag="VHV99_16570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3637065.1"
                     /translation="MTEQEIDKASSFEAVRARLLALAYRMLGSRAEAEDVVQDVWLKW
                     HLAETQEVQTPAAWLTTLTTRVAIDRLRHVQRERASQAAGWLPEPWLDEVAPSAEELA
                     LRAAEMSYGVMLLLERLKPDERAAFVLHEAFDCDYAEIAKIVDRTPASCRQIVRRAKA
                     RLQRAGAPLERPDPAAHARIVERLRTALEAQDRVGLLRLFSDAPDVVSDVPVSTQELA
                     PVAWMETVTALAHQASHAEVVSVEGAMCIALLSEGEVVGVIDVSTQSLADGSVKIVAL
                     RAVTSAVRLQAANRLLGRAAVMKLLARIQQNTMASITMRSDFPVDVDA"
BASE COUNT         1112 a         2277 c         2100 g         1324 t
ORIGIN      
        1 atatcgggga gtatgaggcg gggaactgag ccgcagcgat gctggcagcc agggcagcta
       61 cgttatgcgg ttgccgcgct tgtctgccgc gtcacgccac gcccaaccag cggggcaacc
      121 agttcagaat cggcgaaccg cgcaatggcc gcgttgctgc acgttctgag ccgctcgcca
      181 gcgcgaaacg cgcaccggcc tgccctgcac tgatccacgc caccgtgccc cgcggcaact
      241 cggtcgattg cccggttgcc agccagtgat ccatgttgtc accttcgacg gtcagccaga
      301 tctcgcccga aatcaccttg aagatcgacg gacgcgcgac acgccaggcc gccgcgggct
      361 cgccatgttc cagttcaaaa atacggactt cacgcatcgc tgctctcctt caaagaactt
      421 tgctgttgta ccgattattg acgtgcgatc atcggataac catgcacagt tccgaacact
      481 tctatcggaa ctgttcagtc gaaaaactgg acagtgggct atagatgcgc tttaggtggg
      541 cgtgtgcacg cagcgcgtat ggcgcgcggt gtgcgtcgtg ttgctgggtg gctgggtggc
      601 tgggtggctg ggttaccgtg tcggcgtgtt gccgtgttgc cgtgttgccg tgttgccgtg
      661 ttgccgtgtt gccgtgctgc cgtgctgccg tgctgccgtg ctgccgtgtc agcgttccgg
      721 cgttccggcg ttccggcgtt gcggcattcc ggctgaacgg ctatccggtg aaggagaaga
      781 accgcatgaa actcgaaatt caactcgatc gggataacgg cgttccgctg accgagcaga
      841 tcgtcaccgg cgttaccgca tggattcgtt cacgcactgc ccaccccggc gcaaagctgc
      901 cgtcgatccg tcagttcgca gccgactacg gcgtaagccg tttccccgtg atcgaggcct
      961 acgaccggtt ggtgtcgctc ggctatgtcg actcgcgtca cggctccggc ttttatgtcg
     1021 ccgaccgtct gccggcgagc acgcaatgcc agggcacctc ggacccgcgc cgcgccgagg
     1081 acgaatcggg gcacatcctc caacagttca accacccggg cgagacgctc aagctcggca
     1141 gcggcttcat ccccgatgcg tggcgcgaca tggacagcct cgcgcaagca atccgccacg
     1201 tgtcgcgcac cgacaccgca agcatggtcg attacgcgac cccgctcggc aatctgacct
     1261 tgcgcgaaca tctgcaaggc cgcatcggtc agttggggat tcaagcggac gcttcgcaga
     1321 ttctgatcac caacggcgcg agccaggcgc tcgatctgct gatgcgctac atgctcaaag
     1381 ccggcgacac gatgttcgtc gaggaccccg gctactacaa cctgtacggt ctgctgaagc
     1441 tgcacggcgt caagctgatc ggcattccgc gcacccgcaa cggccccgat ctcgatgtgt
     1501 tgcaggcgca gttgcaactg caccggccga agctgatgtt catcaacacg gtgttccaca
     1561 acccgaccgg caccacggtc gcgccgccgg tggcattccg gctgttgcaa ctggcacgtg
     1621 agcacggctt cgccatcatc gaagacgaca tctacgccga cttccagacc gacgtcaccg
     1681 accgtctcgc gaccctcgac caactcgaac gcgtgatcta cgtaggcggt ttgtcgaaga
     1741 cgctgtcgtc ctcgctgcga atcggctgcg tggtggcgaa ccacgcgatc atcaaggatc
     1801 tgatcgacat caagatgctg acgagtctcg gcgcttcgcg ctttgccgag gcggtcgccg
     1861 tgtcgctgct ggagcgcggc gcctatcgca agtatctgga gcgcctgcgc cgccgcatgc
     1921 gcgacgcgct cggctcgacc atccagacac tcgaagacgc cggctgggag gtgttcgaaa
     1981 agcccgtcgg cggcaagttc gtgtgggcgc gcgtgccgca cgtcgacaac gccgaacggc
     2041 tggtcgcatg cggtgcgccg ctcggcgtga cagtgttgcc cggcagctat ttccggccga
     2101 acatggaagt gagcccgtgg atccggattc acgcggcatt cggcaacgag ccgcgtgcgc
     2161 aggcgttttt ccttgctgcc gcggcgttgc ctgcgtcggg gtgatctgct ggccgaacct
     2221 tcgcttgcgt tgcctgcgtc ggggtgatct gctggccgaa ccttcgcttg cgttgcctgc
     2281 gtcgggctga tctgctggcc cagccttcac acttaatgcg taccctcggg caaaagagcc
     2341 gcctggctga gcgcccgtaa tagcgctgcg ccctgcacgg aagcgcgcaa ccgtcgtggc
     2401 gcgtgctcac gtcgtgccag atcgaacctc aagccagccg atccatccga aatacccgct
     2461 gcgttgggcc acgcttttcc ctagaatagc gagtccagcc cgtccctgag cgccccgccg
     2521 ccgttcatgt ccacgccgcc gaccctgccc gctgccgccg ctgctgccgt gccaacccct
     2581 tcggcccgct cgctgaccgt gatgctgtgg ctcgtcgcca ccggcttctt catgcagacg
     2641 ctggactcaa ccatcgtcaa caccgcgctc cccgcgatgg cgacgagcct cggcgagttg
     2701 ccgttgcgca tgcaatcggt tgtgatcgcc tattcgctga caatggcggt gatgattcca
     2761 gtgtccggct ggctcgccga caaactcggc acccggcgcg tatttttcag cgcgatcctc
     2821 gtgttcgggc tcggctcact gctgtgcgcc aacgcgcatt ccctgaatca actggtgatc
     2881 taccgcatca tgcaaggcgt gggcggggcg atgctgctgc cggtcggccg gctggcggtg
     2941 ctgcgcaccg ttccggcgga acgctacttg tccgccttgt cgttcgtggc gattccaggg
     3001 ctcattgggc cgctgatcgg cccgacactc ggcggctggc tcgtgaagat cgcatcgtgg
     3061 cactggatct ttctgatcaa cgtgccggtg gggatcgcag gttgcatcgc cacgtttatc
     3121 tttatgccgg acagccgtaa cgagcacacc ggcaagttcg atctgaaagg ctatttgctg
     3181 ctgatcgtcg gcatggtgac gatttcgttc gcgctcgacg gccgcaccga gtttggtatt
     3241 caacacgcca ccgtgctggt gctgctgatc ctgagccttg ccagcttcgt cgcctatggt
     3301 ttgcacgccg tgcgcgagcc cgcgccgatc ttcccgctcg acctgttcaa aatccacacc
     3361 ttcagcgtcg gtctgctcgg caatctgttc gcgcgaatcg gcagcggcgc gatgccgtat
     3421 ctgattcctc tgctattgca ggtgagtctc ggctatagcg ccttcgaagc cggcatgatg
     3481 atgctgccgg tggcggcagc aggcatggtc gccaaacgcg ttgtcacgac gctcatcgtc
     3541 aaatacagtt accgacgcgt gctgatcatc aacacggcgc tggtcggcct gaccatggcg
     3601 agctttgcac tcaccagtgc cggccagccg ttgtggttga ggctcgtgca actcgcgttc
     3661 ttcggtggcg tcaattcgat gcagttcacc gcgatgaaca cgttgacgct caaggacctc
     3721 ggcaccggtg gcgcgagcag cggcaacagc ctgttctcgc tggtgcagat gctgtcgatg
     3781 agtctcggcg tgacggtggc aggcgcatta ctcgccacct tcaccggcct gctgccgcgc
     3841 gtgacggccg ccaactctct gcccgcgttc catgcgacgt tcctctgtgt cggcattatc
     3901 accgcgggca cgtcgtggat cttcgcgcaa ctgtcgccgg acatccgcac gctggggaca
     3961 aagactgatc cttcggagcg gacctgagtt ccgcgcgggg ggtggtgacg atggcgtgat
     4021 tcgctatggc tcgctgtgac ccactgttag gacccgctga ggctcattgt tggaacccgc
     4081 tgcggatcac tgggacccgg gcgcggcccg atgccattcg gcacgattcg ttctgactcg
     4141 tcgccgtctc ccgtgaccgc cgcaaccctc ttcgcggtta ttcgctacgg ccgccgccgc
     4201 cgacccgcaa cggggcgtga gtcgcaccac cgaagtgcga atcatcgctt tcgcacccgg
     4261 gctggcgcac aatcgttcga ctgaccgtgg caacccggcg cgcacagtat gtttccgctc
     4321 gcgcatagtt cttgctcgcc tatcctgtaa actcgcattt tgcgcgtgcc ggctcatatc
     4381 gagcatgcga gcgcgccgcc acctccacac gactgacatg atgagcatga tcgaactcga
     4441 tcctcccggc ttccagccgc cgcgcccgat ggccggcgac gagggctccc ggcgcaccgt
     4501 tctgtacggc ggctacaccg tttttagcgt gttccagcca gttttctcgg tgtcgcaccg
     4561 gcgtgcgatc ggttatcacg cttcgctgcg cgcacacgac gaacatgcgc tgcaagtgcc
     4621 gtcgcacgag gttttcacgc aggcggcgcg gcgcggcgac ctgctggaac tcgggcgtct
     4681 cgccgaatcg ctgcatctgg gcaatttcaa tgcattcgac agccacgacg aatggctttt
     4741 cctgagcctg cacccggcag cattgatgga caccagttac ggcgacgcac tgctcgccgg
     4801 cctcaaggcg ctgggcctgc cgccgcaacg ggtggtgctg gaagtgtccg agcaggccgg
     4861 cggtgaaacc acgcgcttcg ccgaaatcgt cgacgcgttg cgcaagtccg gcttcctgat
     4921 cgcgctggac ggcttcggcg cgaagcattc gaacatcgac cgcgtgtgga atctgcggcc
     4981 ggacatcgtc acgctcgacc gctgcatcct cgcgcaagcg agcgagcact cgcacatcga
     5041 acgcgtgctg ccgggccttg tctcgctgct gcacgaatct ggccaactgg tgctgatggg
     5101 cggcctgagc accgagcgcg atgcgctgat cgcgctcgaa tgcaacgtcg acttcgtaca
     5161 gggtgcgttc ttcgcaggcc ccagcgtcga accggtgaaa ccgcaagcgg cggccggttt
     5221 gatggactcg ctctcagcgg cgctacgcga acgggttgcg acgcgcgaac gcgtacaatc
     5281 cgcgcggctt gcgccctatg tcacggcgct ggaaacggcg gctgcgcaac tcgtggccgg
     5341 ggaatcggtt acccaggcga ccgcgccgct gctcacgctc ggcgaaaccg cgcgctgctt
     5401 cgtactcgac ggttcggggc ggcagatcgg cgacaacgtg ctgccgccgg ggcgtgcttc
     5461 gcaacgcgcg aaacgcttcc ggccgttact gcattcggaa ggcgcgagct gggagcgccg
     5521 gccgtacttc attcaggcga tgcgcatgcc cggccgggtg cacctcaccg cgccctacct
     5581 gtcgatcaac gaggcgcact tgtgcgtgac ggcctcgatc gccgcgcaca cgccgcacgg
     5641 cactcaggtg ctatgcgtcg acatcaactg ggaagtcgct acgcatcgta attgaggcca
     5701 tggtgttctg ctggatccgc gcgagcagct tcatgacggc cgcgcgaccc agcaagcggt
     5761 tggctgcctg caaacggacc gcactggtga cggcgcgcaa cgcgacgatc ttcaccgaac
     5821 cgtccgcaag actttgcgtg gaaacgtcga ttactccgac aacctcgcct tcggagagca
     5881 acgcaataca cattgcgcct tcgaccgaca ccacctctgc atggctcgcc tgatgcgcaa
     5941 gcgcggtcac cgtctccatc caggccaccg gcgccagttc ctgcgtgctc actggcacgt
     6001 cgctgaccac gtcgggcgca tcgctaaaga gacgcagtaa accaactcga tcctgagcct
     6061 ctagcgctgt gcgcaggcgc tccacgatcc gcgcatgcgc ggcaggatcg ggccgctcca
     6121 gcggcgcacc cgcacgctgc aaacgcgcct tcgcacggcg aacgatctga cggcaactgg
     6181 ccggcgtacg gtcgacgatt tttgcaatct ccgcgtagtc gcaatcgaaa gcttcgtgca
     6241 gtacgaatgc cgcccgctca tccggtttca gacgctcgag cagaagcatc acgccgtacg
     6301 acatctccgc ggcgcgcagg gccagttcct cggccgatgg cgccacctcg tcaagccagg
     6361 gctccggcaa ccagccagcg gcctgtgacg cgcgttcacg ctgtacgtgg cgcagccggt
     6421 caatcgccac gcgcgtagtg agcgtggtga gccaggccgc cggcgtctgc acctcttgag
     6481 tctcggcgag atgccatttg agccagacgt cctgcaccac gtcttccgct tcggcccggc
     6541 tgccgagcat ccggtacgcc agcgcgagca ggcgggcgcg cacggcctcg aagctggatg
     6601 ccttgtcgat ttcttgttcc gtcatgcgta gacctccaga tcgtggatgg tgaaaaagcg
     6661 cgcgacgcca tgcgtgcttg tctagttagc tgcctgccta gctcgcgtgc ctgcgtactc
     6721 gcgtgcctgc gtgcctgcgt gcctgcgtgc ctgcgtgcct gcgtgcctgc gtgcctgcgt
     6781 gcctgcgtgc ctgcgtgcct gagtgcctgc gtg
//
LOCUS       DATASH010000608         2526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5836741,
            whole genome shotgun sequence.
ACCESSION   DATASH010000608 DATASH010000000
VERSION     DATASH010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5836741"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VHV99_16575"
     CDS             <1..180
                     /locus_tag="VHV99_16575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637066.1"
                     /translation="GLAVYLPEHFCRTPDCAKAHPAFLVNVVPIALGVADGLSPVSAV
                     FPQIIFPAFLPKIRS"
     gene            416..1255
                     /locus_tag="VHV99_16580"
     CDS             416..1255
                     /locus_tag="VHV99_16580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX3637067.1"
                     /translation="MRISLSTVLRFFHTPALTLISVFGLALALSTAPATAADTPTLKI
                     GTATSPQIEALKVAAREAKEQGLDVKIIEFTDWNTPNAALANKDIDVNYFQHIPFLEN
                     ARKQGGYNFIAIAPGTIMKIGLYSKKIKRFDELKDGATVAIANDPVNGGRGLLLLQRA
                     GLIKLTPGIDYRATTLDIIDNPKHLKIVQLEASQLARSLDDVDLAQGYPSFIKLAGTA
                     DPNSALLFDGLENKNYAIQWVVRPESANDPRIRKFISIYQHSPAVRAALDKAFGNLYA
                     VAW"
     gene            1362..>2526
                     /locus_tag="VHV99_16585"
     CDS             1362..>2526
                     /locus_tag="VHV99_16585"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX3637068.1"
                     /translation="MAKKKILLNAFNMNAVGHINHGLWTHPRDRSAHYTDLDYWTSLA
                     QTLERGKFDGIFLADIVGVYDVYQGGPETPLRESVQIPINDPSLIVPAMAHVTKHIGF
                     GVTSNLTYEPPYLFARRMSTLDHLTKGRVGWNIVTGYLDSAARGMGLAQQISHDDRYD
                     RADDYMDVVYKLWEQSWEDDAVVRDRAARIFSHPGKVHRVKHDGPYYSIDAIHLSEPS
                     PQRTPVLYQAGSSSRGVDFAARHAECVFVGGQNKQLTRSIVDDIRARAVSFGRAPDDI
                     RIFAGITVVVDETERAAQEKFEEYRRYASAEGGIAHFSSSTGIDFSQYELDEPISYVK
                     TESMQSAVEAISKKSVSGVWTKRKVLEQMTLGGRAKPVVGSPQQIADELVSSAR"
BASE COUNT          535 a          782 c          710 g          499 t
ORIGIN      
        1 gggcttgctg tctatctgcc agagcatttt tgtagaacgc cggactgcgc gaaagcgcat
       61 ccggcgtttc tcgttaacgt tgtgccgatc gcactcggcg tggcagacgg cttatcgcct
      121 gtttccgccg tgtttcctca aatcattttt cccgctttcc ttccaaaaat ccgttcgtag
      181 accgctggtt tcccgccgtc tatcatgact cgcacctcat ctggagagag gcgatgacct
      241 gtccgacttg ccgctgtccg cgctaatccg ctcgcgcgtt ctattgctgc tgcacgccgc
      301 ttaacccgcg cgccgcagat ttccgttgcc cgatcttttc aggcatgcgt tgccgcatgt
      361 cgttggctcg gcacgcctcg agaaacacct cgccccaaat ttaaacgagc tcaccatgcg
      421 catatctctg tccaccgtgc tgcgtttctt ccatacgccg gctctaaccc tcatcagcgt
      481 gttcggcctc gcgctggcgt tgtccaccgc gcccgcaacc gcggcagata cgcccacatt
      541 gaaaatcggc accgccacca gtccgcaaat cgaagcgctc aaagtggccg cgcgcgaagc
      601 gaaggagcag gggctcgacg tgaagatcat cgagttcacc gattggaata cgccgaacgc
      661 ggcgctcgcc aacaaggata tcgacgtcaa ctacttccag cacattccgt ttctcgagaa
      721 cgccaggaag cagggcggct ataacttcat cgcaatcgca ccgggcacga tcatgaagat
      781 cggtctgtac tcgaaaaaga tcaaacgctt cgacgaattg aaagacggcg ccacggtcgc
      841 cattgctaat gatccggtca acggcggtcg cggcctgctg ttgctgcaac gcgccggtct
      901 catcaagctg acgccgggta tcgactatcg cgccaccaca ctcgacatca tcgacaaccc
      961 gaagcatctg aagatcgtcc aactcgaggc gtcgcaattg gcgcgttcgc tggacgacgt
     1021 cgatctcgcc caaggctacc cgagcttcat caagctcgcg ggcaccgccg atccgaatag
     1081 cgcattgctg ttcgacggtc tggaaaacaa gaactatgcg atccagtggg tcgtgcgtcc
     1141 ggaaagcgcg aacgacccgc gtatccgcaa gttcatctcg atctatcagc attcgcccgc
     1201 tgtgcgcgcc gcgctcgata aggccttcgg caacctctat gccgttgcct ggtaagttac
     1261 gccgccaagt tacgccgcca ggctatgcaa ctgagcagcg caactgaacc acgcaaccat
     1321 gccacgcacc cagcaacgtt gtaatagcaa ggagccacac gatggcgaag aaaaagatcc
     1381 tgctgaacgc gttcaacatg aacgcggtcg gccacatcaa tcatggcttg tggacgcacc
     1441 cgcgagaccg ttccgcacac tacaccgacc tcgactactg gacgagcctc gcgcaaacgc
     1501 tcgagcgcgg caagttcgac gggatctttc tcgcggatat cgtcggcgtg tacgacgttt
     1561 accagggcgg cccggaaacc ccgttgcgcg aatcggtgca gattccgatc aatgatcctt
     1621 cgctgatcgt gcccgccatg gcgcatgtga cgaagcatat cggcttcggc gtcacatcga
     1681 atctgactta cgaaccgccg tatctgttcg cgcgccgtat gtcgacgctg gatcatctga
     1741 ccaaaggcag ggtgggatgg aacatcgtca ccggctatct ggacagcgcg gcgcgcggca
     1801 tgggtctcgc gcagcagatc agccacgatg accgatacga tcgcgccgac gactacatgg
     1861 atgtggtcta caaactatgg gagcaaagct gggaagacga cgcggtggtg cgcgatcgcg
     1921 cggcacgtat cttctcgcat cccggcaagg tgcatcgggt gaaacacgac ggtccgtact
     1981 attcgatcga cgcgattcat ttgagcgagc cgtcgccgca acgcacgccg gttctgtatc
     2041 aggcgggttc gtcgagccgg ggcgtcgatt tcgcggcacg tcacgcggaa tgcgtgtttg
     2101 tcggcggcca gaacaagcag ctcacgcgct ccattgtcga tgacattcga gcacgagcgg
     2161 tgagcttcgg gcgcgcgccc gacgatatca ggatctttgc cggcatcacc gttgtagtcg
     2221 acgaaacaga acgcgccgcg caggagaaat tcgaggagta ccggcgttat gcaagcgccg
     2281 agggtggcat cgcgcatttc tccagttcca ctggcatcga cttctcgcag tacgaactgg
     2341 atgaaccgat ttcgtatgtg aagaccgaat cgatgcaatc ggcggtcgaa gcaatttcga
     2401 agaagagcgt gagcggcgtt tggaccaaac gcaaggtgct cgagcagatg acactcggtg
     2461 gccgcgcgaa gccggtggtc ggttccccgc agcagattgc ggacgaactc gtgtcatctg
     2521 ctcgag
//
LOCUS       DATASH010000609         2214 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5640664,
            whole genome shotgun sequence.
ACCESSION   DATASH010000609 DATASH010000000
VERSION     DATASH010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5640664"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            370..1023
                     /locus_tag="VHV99_16590"
     CDS             370..1023
                     /locus_tag="VHV99_16590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX3637069.1"
                     /translation="MANTTLTISSKNYSSWSLRGWLLTKFSALPFEEIVMPIDDPAAR
                     AELLLLSPSILVPCLCHDGIKIWDTLAIAEYLNELKPEAALLPKDIRARAHCRSICGE
                     MHSGFGSLRSALPMNLKAHFPGFKVWARAQSDIDRIVTIWSECLKQYDGPFLFGERSA
                     ADAMYAPVVTRFVTYDVKLDPEIVDYGQRIMALPEMQQWIADAQQEVEEIDELDVEF"
     gene            complement(1208..>2214)
                     /locus_tag="VHV99_16595"
     CDS             complement(1208..>2214)
                     /locus_tag="VHV99_16595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX3637070.1"
                     /translation="ALETAHRVNIVAFDKTGTLTVGQPSVTAFEAIGDIGRDEAMALA
                     AAVQRHSDHPLARAVLKAYEEAKLATRSPITTLAASAARAVPGRGVEADIDGRTLALG
                     STRWLNELGIPLAPQLAERAKQLEAAGNTVSWLMQRAPLAPVALALIAFGDTVKPNAR
                     AAVERLAQMGIKSVLVTGDNHGSAASVAKALGIAEFHAEVLPDDKARVIRDLKIRSAG
                     IVAMVGDGINDAPALAAADIGIAMATGTDVEMQAAGITLMRGDPALVADAIDISRRTW
                     RKIQQNLFWAFVYNLIGIPLAAFGLLNPMLAGAAMAFSSVSVVTNALLLRSWRGALMR
                     "
BASE COUNT          392 a          696 c          717 g          409 t
ORIGIN      
        1 ctcgcaggcc gcgcgtagcg aacgtccatg cgacgtatcg ccgaatgcgg gccatgccta
       61 gagcgagtga ttcaccagcg ctgcgccgtg ccgcatcggc agcatcatgt cggcgaaagc
      121 ctggtatgtg ggatatgcga acaggttcat aaccttcgca cgaatgcatt aaaggaagga
      181 gcccgaatag aggcaagtca tgtgccaatc atgcgctcgc actggcgccg ctgtccctgc
      241 acgttggcat agcatttgca cgggttaggc cagcgggccg aaattgccgc gctttggtgc
      301 gcccgcgcac ggcagcgtgc acgcgcatcg tcaggcgtcg gcggagcgcc tcatctgggg
      361 gagaaacgta tggcaaatac cacgctcacc atcagcagca agaattattc gtcgtggtcg
      421 ctgcgcggct ggctgctgac caaattcagc gccttgcctt tcgaagagat cgtgatgccg
      481 atcgacgacc ctgcggcgcg cgccgaattg ctgctgctct cgccgtcgat tctggtgccg
      541 tgtctctgtc acgacggcat caagatctgg gacacgctgg cgattgccga atatctcaac
      601 gagttgaaac cggaagcggc gctattgccc aaggacatcc gcgcgcgagc acattgccgc
      661 tcgatttgcg gtgagatgca ttcgggtttc ggttcgctgc gctcggcgtt gccgatgaac
      721 ctcaaagcgc acttcccggg cttcaaggta tgggcgcgcg cgcagtccga tatcgaccgc
      781 attgtgacga tctggagcga gtgcctgaag cagtacgacg gaccgtttct gttcggtgaa
      841 cgcagcgcgg cggatgcaat gtacgcgccg gtggtgacgc gtttcgtcac ctacgacgtc
      901 aagctggacc cggagattgt cgactatggg cagcgcatca tggcgctgcc tgaaatgcag
      961 cagtggatcg cggatgcgca gcaggaagtg gaggagatcg atgaactgga tgtggagttc
     1021 tgaacgcctt aatcgcgccc cgcttccagc ttgaacacac tcaccacctg cgcaagccgc
     1081 gtagcctgat cgcgcagttc agccgccgcg cgtttcgcct cgccgacgat cgtcgcattc
     1141 tgctgggttg cctcgccaat ctgcgtgaca gaaagtcagc aagccaacgc gccaacgcgc
     1201 caacgcatca acgcatcaac gcaccgcgcc agctgcgcag cagaagcgca ttggtgacga
     1261 cgcttacgct cgaaaacgcc atcgccgcgc cggccagcat cgggttcagc aagccgaagg
     1321 cggcaagcgg gatgccgatc aggttgtaga cgaacgccca gaacaggttc tgctggatct
     1381 tgcgccaggt ccggcgcgaa atgtcgatgg cgtccgccac cagcgcgggg tcgccgcgca
     1441 tcagcgtgat gccggccgcc tgcatctcga cgtcggtgcc ggtcgccatc gcgatgccga
     1501 tgtcggcggc agcgagcgcg ggggcgtcgt tgatgccgtc gcccaccatt gcgacgatgc
     1561 ccgcgctgcg aattttcagg tcgcggatca cgcgggcctt gtcgtcaggc agaacttcgg
     1621 cgtgaaattc ggcgatgccg agcgccttgg cgacgctcgc cgcgctgccg tggttgtcgc
     1681 cggtgacgag cacgcttttg atgcccattt gggcgagccg ttcgacggcc gcgcgggcgt
     1741 tcggtttgac ggtgtcgccg aaagcgatca gtgcgagcgc gacgggtgca agcggcgcgc
     1801 gctgcatcag ccaggagacg gtgttgccgg cggcttcgag ctgttttgcg cgctcggcga
     1861 gttgcggtgc caacgggata ccgagctcgt tgagccagcg tgtgctgccg agcgcgaggg
     1921 tgcggccatc gatgtcggct tcgacgccgc gcccgggcac cgcgcgcgca gcgcttgccg
     1981 cgagtgttgt aatcggcgac cttgtcgcga gcttcgcttc ttcatacgcc ttcaacaccg
     2041 cccgcgccaa cgggtgatcg ctatgccgct gcacggccgc ggcgagcgcc atcgcctcat
     2101 cgcgtccaat atcgccgatc gcctcgaacg ccgtcaccga cggttgcccg accgtcagcg
     2161 tgccggtctt gtcgaacgcg acaatattca cccgatgcgc ggtttccagc gcct
//
LOCUS       DATASH010000610        12523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2672128,
            whole genome shotgun sequence.
ACCESSION   DATASH010000610 DATASH010000000
VERSION     DATASH010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 12523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12523
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2672128"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1247)
                     /locus_tag="VHV99_16600"
     CDS             complement(<1..1247)
                     /locus_tag="VHV99_16600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018431750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX3637071.1"
                     /translation="MLTLKRFANPDVTRAFTNIVWLGLERLTQIGVAIVISGMLARYF
                     GPDTFGKWQYANTLLLVLSPITWVCGAEILVPTIVGRAPTQLGTVLGSAFTLRFSVSA
                     VALLITWLGIALHFFDPLVGAMLAGLAVTMLFREPFVGVINAWLQSMTYSKPQLLTSM
                     STALVKAGFVYLLVRAMATPARFGWLWAAESALIGAVLLIYYIRRHGGKLGWHLDRSL
                     FKHFATAGTVFWLGLICMYLFLKLDRLMLERTISFADLGRYSAAQQLNENWITLALML
                     AQTIAPAFVYRVQDAAQLRRNVWRLTAMTAALMVSGAIVLDLLAGFIIRRVFGPGFEG
                     AIDIFRWAVWLSVPAGIEAIGNLIVLKYQAKFVLLSKWLLALAVAFAVNLLAIPRLGA
                     CGALVGLAAGYLAAASINLYYIR"
     gene            1594..2988
                     /locus_tag="VHV99_16605"
     CDS             1594..2988
                     /locus_tag="VHV99_16605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014897868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEX3637072.1"
                     /translation="MISKSIFKAYDIRGVIGKTLDADAARSIGRAFGSEVRAQGGDAV
                     VVARDGRLSGPELIQALSDGLRAAGVDVVNVGMVPTPVGYFAASVPLQLDGGERRVDS
                     CIVVTGSHNPPDYNGFKMVLRSAAIYGEQILALHQRIVDENFSEGSGTYTEYDIADAY
                     LDRIASDVKLARPIKIVVDTGNGVAGGLAPKLFKKLGCELVELFTEIDGNFPNHHPDP
                     AHPENLQDVIRALKETDAEIGFAFDGDGDRLGVVTKDGQIIYPDRQLMLFAEEVLSRN
                     KGAQIIYDVKCTRNLAKWVKDKGGEPLMWKTGHSLVKAKLRETGAPLAGEMSGHVFFK
                     DRWYGFDDGLYTGARLLEILTRVADPSKLLNSLPNSNSTPELQLKLEEGENFELIARL
                     QQNAKFTGADDVVKIDGLRVEYPDGFGLARSSNTTPVVVMRFEADSDEALRRIQEDFR
                     RVIMAEKPDAKLPF"
     gene            3209..4210
                     /gene="waaC"
                     /locus_tag="VHV99_16610"
     CDS             3209..4210
                     /gene="waaC"
                     /locus_tag="VHV99_16610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HEX3637073.1"
                     /translation="MSVQKILIVRVSSLGDVVHNMPVIADIRRRHPEAQIDWLVEESF
                     VGLVELVSGVRRAIPVSLRRWRKRILSIDNWREIGAFRRALAAENYDLVIDCQGLIKT
                     AWVASMARGPVVGLANRTDGAGFEWPVRFFYDKRVPIEARTHVVERTRQLVAAALNDP
                     QPQPTDDIDFGLDTGRAALALSEANLNLPVPYVVFVHATSRADKQWPATAWIELGQSL
                     VRRGASIVLPWGSEEERVTSERLAKEFGAAAIVPPRLALPAVVGLIEGAAATVGVDTG
                     LVHIAAALKRPTVELYNFATAWRTGGYWSPNVINLGTAGKPPTLQEVKSALAGFGLL"
     gene            4272..5156
                     /locus_tag="VHV99_16615"
     CDS             4272..5156
                     /locus_tag="VHV99_16615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017332912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo hydroxylase family protein"
                     /protein_id="HEX3637074.1"
                     /translation="MNETQIIEVANADWHGRNLSVPREMLLAGVERGKVLYFPNLRFA
                     IEGGEQALLDPALADPNRKNISLEPNGGALHGVAGDAVTQSAVRALIARYQANARTLV
                     DGLFPEYNGRLRVAPTSLRLHQVEGRETSWRKDDSRLHVDAFPSRPNYGERILRVFTN
                     VNPHGAPRVWRVGEPFEDMARRFLPRIKPQMPGSAWLLNLLHVTKSPRSEYDHLMLNL
                     HDSMKADLDYQKSSPQETMPFPPGSVWVCFSDQTSHAVMSGQFMLEQTFFLPVKAMAQ
                     PDCAPLGILERLKGRALV"
     gene            5263..6579
                     /gene="waaA"
                     /locus_tag="VHV99_16620"
     CDS             5263..6579
                     /gene="waaA"
                     /locus_tag="VHV99_16620"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018431747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HEX3637075.1"
                     /translation="MLRAIYNALWWIIAPLAVLRLLIRSRKERGYREHIGERFGYSRG
                     RLPEDNAPLIWVHAVSVGETRAAQPLIDALMKARPDARVLLTHMTPSGRATGEQIFGD
                     RVLRSYLPYDMPHAVRRFLRAWRPSLGLVMETEVWPTLIDECRRADVPLVLTNARMSA
                     RSFKRAAKFGHATRDVFGGFARVLAQSPSDAERLTALGARNVAVLGNLKFDMSTPAEL
                     AARGHAWRAAIGTRPVWVAASTREGEEELVLQAFAALGVDDALLILVPRHPQRFNEVA
                     ALAEKARLRLERRSTWAPAAAVASAAAAAAGGGVPPLPSDVKVLLGDSMGELGAYYAA
                     SDLAFIGGSLLPLGGQNLIEACAVGVPVLIGPHVFNFTQATADAVAAGAAVQVQDPAD
                     LARVLRELFSDKARRLAMGGAASAFAARHRGATARTVDVLMAVLPE"
     gene            complement(6642..7295)
                     /gene="ureG"
                     /locus_tag="VHV99_16625"
     CDS             complement(6642..7295)
                     /gene="ureG"
                     /locus_tag="VHV99_16625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HEX3637076.1"
                     /translation="MNAPHQATHSSVRTKKLPPLRVGVGGPVGSGKTTLLEMLCKAMR
                     EQYDLVAITNDIYTKEDQRLLTVAGALPAERIMGVETGGCPHTAIREDASINLEAVDR
                     MLTRFPDADIVFIESGGDNLAATFSPELSDLTIYVIDVAGGEKIPRKGGPGITKSDLL
                     VINKTDLAPMVGANLEVMASDAKKMRGERPFVMCNLKALDGLDVVVKFIEEKGLLKV"
     gene            complement(7307..7987)
                     /locus_tag="VHV99_16630"
     CDS             complement(7307..7987)
                     /locus_tag="VHV99_16630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015601275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HEX3637077.1"
                     /translation="MRIAELTALLHLASPALPIGAFSYSQGLEAAIEAQLITDADSAR
                     DWIASGLTDVLARGELPFLAHQMKRWREHDADGLKEANSEFLASRESAELRRETEQMG
                     WSLRQLCVSLEWGDAARRATLSSLKPLAQPTAFAFAAFAHDAAPDAALAAYAFSWVEN
                     QAAAALKAVPLGQLAGQRIIVALREPIDAAVTRALATSPDNINTFAPQLGILSARHES
                     QYSRLFRS"
     gene            complement(7974..8756)
                     /gene="ureE"
                     /locus_tag="VHV99_16635"
     CDS             complement(7974..8756)
                     /gene="ureE"
                     /locus_tag="VHV99_16635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HEX3637078.1"
                     /translation="MRTIDKLIAPHLKLAPVLVKRAPTLTLAFDDRRKSRLAATLDNG
                     EDVALLLPRGTVLRDGDVLVADDGGLVRVVAAPETVLYVRAKDALTLTRAAYHLGNRH
                     TPVEVGADYLKLEYDPVLGDMLKRIGASVDQVSMPFQPESGAYGGGHKHGHDETFAED
                     YAIAQQVFGEHHGHEHSHDHEHGNAHGHSHDHEHGHDHSDHEHGHSHAQGHGHDQPDH
                     EHGHSHAQGHAHDHDHSHGHNANDHVHDESCGHGHHHHHAHR"
     gene            complement(8760..10466)
                     /gene="ureC"
                     /locus_tag="VHV99_16640"
     CDS             complement(8760..10466)
                     /gene="ureC"
                     /locus_tag="VHV99_16640"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006758739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HEX3637079.1"
                     /translation="MTLRIGRRAYAEMFGPTTGDRIRLADTELLIEVERDFTTYGEEV
                     KFGGGKVIRDGMGQSQRVHADVVDTVVTNAVILDHWGIVKADIGIKNGRIFAIGKAGN
                     PDIQPNVTIAIGASTEVIAGEGLIVTAGGIDTHIHFISPQQIEEALASGVTTMLGGGT
                     GPATGTNATTCTPGPWHLERMLQAADGYPMNLGFLGKGNVSQPQPALEQIAAGAIGLK
                     LHEDWGTTPAAIDNCLSVADDTDTQVAIHTDTLNEAGFVEATVAAFKGRTIHTYHTEG
                     AGGGHAPDIIKVCGEANVLPSSTNPTRPYTVNTLEEHLDMLMVCHHLDPSIAEDIAFA
                     ESRIRRETIAAEDILHDLGALSMLSSDSQAMGRVGEVIIRTWQTAHKMKVQRGALPED
                     NARHDNFRAKRYVAKYTINPAITHGISHEVGSIEPGKWADLVFWEPAFFGIKPSLILK
                     GGMIAMAQMGDPNASIPTPQPVHYREMFATRGGALGRTSLTFVSQMAADAGVGERYGL
                     NKRIVAVKNCRNITKADMIHNAWRPAISVDPETYQVIADGQLLTCEPATVLPMAQRYF
                     LF"
     gene            complement(10522..10827)
                     /locus_tag="VHV99_16645"
     CDS             complement(10522..10827)
                     /locus_tag="VHV99_16645"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017332918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HEX3637080.1"
                     /translation="MIPGELLIDDGEHELNAGRATVTVVVSNTGDRPVQIGSHYHFYE
                     VNEALSFDREAARGFRLNIAAGTAVRFEPGQERTVELVELAGERVVYGFNGKVMGKL"
     gene            complement(10857..11159)
                     /gene="ureA"
                     /locus_tag="VHV99_16650"
     CDS             complement(10857..11159)
                     /gene="ureA"
                     /locus_tag="VHV99_16650"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HEX3637081.1"
                     /translation="MKLTPREKDKLLIFTAALLAERRRARGLKLNYPEAVAFISAALM
                     EAARDGKTVAEVMHYGTTLLTRADVMEGVPEMIPDIQVEATFPDGTKLVTVHHPIP"
     gene            complement(11321..12124)
                     /locus_tag="VHV99_16655"
     CDS             complement(11321..12124)
                     /locus_tag="VHV99_16655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015001897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX3637082.1"
                     /translation="MTRSTLRPARRAWLAALLLSPVLAVTAPTAHADTLDNIAKAGVL
                     KVAVPEDYPPFGSVGADMKPQGYDIDTAALLAKSMNVKLELVPVNSANRIPYLQTGKV
                     DLVISSLGKTPERDKVIDFSTAYAPYYQGVFGPADIKVTGPADLTGKTVGATRGALEE
                     IGLTQMAPNATIKRFEDNNATIAAFLSGQVQLIAAGNIVAGAILAKNPPRRPEPKFVI
                     KNSPCFVGMNKNEPRLQQKVNAAIAAAKQDGTLNTMSKKWFGAPLPTDL"
BASE COUNT         2252 a         3752 c         4122 g         2397 t
ORIGIN      
        1 aaacgaatgt aataaagatt aatcgacgcg gcggcgagat aaccggcggc gagtcccacc
       61 agcgcaccgc aagcgccgag ccggggaatc gccagcagat tgacggcgaa cgcgaccgcc
      121 agcgccaaca gccacttgga aagcaagacg aacttggcct gatatttcag cacgatcaga
      181 ttgccgatcg cttcgatccc ggccggcacc gaaagccaca cggcccagcg gaaaatgtcg
      241 atggcgcctt caaagcccgg cccgaacacg cggcggatga tgaagccagc cagcaggtcc
      301 agcacgatcg cgccgcttac catcagcgcg gcggtcatcg ccgtgaggcg ccacacgttg
      361 cggcgcaatt gcgccgcatc ctgcacgcga tagacgaagg caggcgcaat ggtttgcgcg
      421 agcatcagcg cgagcgtgat ccagttttcg ttcaactgct gcgcggcgga ataacggccg
      481 aggtcagcga aggaaatcgt ccgctcgagc atcagtctgt ccagtttcag gaacagatac
      541 atacagatca ggccgagcca gaacaccgtg cccgcggtcg cgaagtgctt gaagagcgaa
      601 cggtccagat gccagccgag cttgccgcca tgccggcgga tgtaatagat cagcagaacc
      661 gcgccgatca acgccgattc agcggcccac agccagccaa agcgcgcggg cgtagccatc
      721 gcgcgtacca gcagataaac gaaaccggcc ttcaccaatg cggtgctcat gctggtgagc
      781 aactgcggct tgctgtaagt catgctttgc agccacgcgt tgatcacgcc gacgaacggc
      841 tcgcgaaaca gcatcgtcac cgcgaggccg gcgagcatcg cgcccaccag cggatcgaag
      901 aagtgcaacg cgatgccgag ccacgtgatc agcagcgcca cggcggaaac cgaaaagcgc
      961 agcgtgaagg cgctgccgag cacggtgccc aactgggttg gcgcccggcc gacgatggtg
     1021 ggcaccagaa tttcggcgcc gcacacccag gtgatcggcg aaagcaccag caggagcgtg
     1081 ttcgcgtact gccatttgcc gaaggtgtcg gggccgaagt agcgcgccag catgccgctg
     1141 atcacgatcg ccacgccgat ctgcgtgagc cgttccagcc cgagccagac gatattggtg
     1201 aaggcgcgtg tgacgtcggg gttggcgaag cgtttgagcg tcagcatccg ccggccgctc
     1261 gccaatgtaa cggagcccgt tgtgcggcct gcgccgattg cgcccattgc atgggcggcg
     1321 cctgcgacgc gaggagcgca ctgtgagtac tgtgcgcaat tgcccacccg gccgtctgtc
     1381 gcggcaactt tacaaaagga gggcgtctaa gcattagaat ggcggttata agactggtcc
     1441 gagaagcgta attataagtt ggaaccggat cccttccggt agaggcgctt cgcgatgtct
     1501 tatccgttgg tgaatagcga attagccgct caatttaccg atcccccagc gacgcgccgg
     1561 tcaaaatttt ctattgcacg caagtgagcc aacatgatct ccaaatctat tttcaaggcg
     1621 tatgacattc gcggtgtgat cggcaagacg ctcgacgccg atgcagcgcg ttcgatcggc
     1681 cgcgccttcg gcagcgaagt gcgggcgcaa ggcggcgacg ccgtggtggt cgcgcgtgac
     1741 ggacgcctgt caggtcccga actgatccag gcgctgtcgg acgggctgcg tgcagccggc
     1801 gtcgacgtgg tcaacgtcgg catggtaccc acgccggtcg gctacttcgc ggccagcgta
     1861 ccgttgcagc tcgacggcgg cgaacgccgc gtcgattcgt gcatcgtcgt gacgggcagt
     1921 cacaatccgc cagactacaa cggcttcaag atggtcctgc gcagcgcggc tatttatggc
     1981 gagcagattc tggcgttgca tcagcgtatc gtcgacgaaa acttctctga aggtagtggc
     2041 acatacactg agtacgatat cgctgacgcc tatctcgatc gtattgccag cgacgtcaag
     2101 ctggcgcgcc cgatcaagat cgtggtggac acgggcaatg gtgtcgcggg cggcctcgcg
     2161 ccgaagctct tcaagaagct cggttgcgaa ctggtcgaac tgttcaccga gatcgacggt
     2221 aacttcccga atcaccaccc ggacccggct cacccggaaa acctccagga cgtgatccgc
     2281 gctctgaagg aaaccgacgc tgaaatcggc ttcgcattcg acggcgacgg cgaccgtctc
     2341 ggcgtcgtca ccaaagacgg ccagatcatc tatccggacc gtcaactgat gctgttcgcc
     2401 gaagaagtac tgtcgcgcaa caagggcgcg cagatcattt acgacgtgaa gtgcacgcgc
     2461 aatctggcca agtgggtcaa ggacaagggc ggcgaaccgc tgatgtggaa gaccggccac
     2521 tcgctggtga aggcgaagtt gcgcgaaacc ggcgcgccgc tggcaggcga aatgagcggc
     2581 cacgtgttct tcaaggaccg ctggtatggt ttcgacgacg gcctttacac cggcgcgcgt
     2641 cttctggaaa tcctcacgcg tgtggccgat ccgagcaagc tgctgaactc gctgccgaac
     2701 tcgaactcca cgccggaact gcaattgaag ctcgaagaag gggaaaactt cgaactgatc
     2761 gcgcgcttgc agcaaaatgc gaagttcacc ggtgcggacg acgtcgtgaa gatcgacggt
     2821 ctgcgtgtcg agtatccgga cggctttggg ctcgcgcgtt cgtcgaacac cacgccggtg
     2881 gtggtgatgc gtttcgaagc cgatagcgac gaagcactca ggcgcatcca ggaagacttc
     2941 cgccgcgtga tcatggcgga gaagcctgac gcgaagctgc cgttctaaaa gctcgagcgg
     3001 cgccgtggtt cgtcatgaac tgcggcagcc gtcgaacgcg gcgcgggttt cacttaccgc
     3061 gccgcgtttt ttcatgtcat ttacccgtgg cgcgctagaa ttgcggtcct ctcctcgtga
     3121 gaatcccctc cggggggctt gtcgcgaagc gacaggttcg ggggctcttt caccgcatct
     3181 tcgggccgga tagccggttt tcccacactt gagcgtgcaa aagatactga tcgtgagggt
     3241 gtcgtcattg ggcgacgtgg ttcacaacat gccggtgatc gccgacatcc gccgccgcca
     3301 tcccgaggcc cagatcgact ggctggtcga agaaagtttc gtggggctcg tggaactggt
     3361 cagcggcgtg cggcgtgcca tccccgtgtc gctgcgccgt tggcgcaagc gcattctttc
     3421 gatcgacaac tggcgcgaga tcggcgcgtt tcgccgcgcg ctcgccgctg agaattacga
     3481 cctcgtgatc gactgccagg ggctcatcaa gaccgcgtgg gttgcgagca tggcgcgcgg
     3541 gccggtggtc ggcctcgcga accgcaccga cggcgcgggc ttcgaatggc cggtgcgctt
     3601 cttctacgac aagcgcgtgc cgatcgaggc gcgcacccat gtggtcgagc gtacccgcca
     3661 gctggtggcc gcggcgctga acgacccgca gccgcaaccc accgacgata tcgacttcgg
     3721 cctcgacacc gggcgcgcgg cgctggcttt gtcggaggcg aatctgaacc tgccggtgcc
     3781 gtacgtggtg ttcgtgcacg ccacctcgcg cgccgacaag cagtggcccg ctaccgcgtg
     3841 gatcgaactc ggtcagtctt tggtgcggcg cggcgcgtcg attgtgttgc cgtggggcag
     3901 cgaggaagag cgcgttacca gcgaacgtct ggcgaaggaa ttcggcgctg cggccatcgt
     3961 gccgcccagg ttggccttgc cggcggtggt cggcctgatc gaaggcgccg ccgcgacggt
     4021 cggcgttgac acaggtctag ttcacattgc ggcggcgctg aagcggccca cggtcgagtt
     4081 gtacaatttc gccaccgcat ggcgtaccgg cggctactgg tcgccgaacg tcattaacct
     4141 cggcacggcc ggcaagccgc ccacgctgca ggaagtcaag tccgcgctgg ccggtttcgg
     4201 cctgctctaa tgttgtcgcg ccgtaatgcg gcgtgcttcg ccttacgctc catccgtttt
     4261 caagggcgac catgaacgaa acccagatca tcgaagtagc gaacgctgac tggcacggac
     4321 gcaatctgtc cgtgccgcgc gagatgctgc tggccggcgt ggaacgcggc aaagtgctgt
     4381 atttcccaaa cctgcgcttt gcgatcgaag gtggcgaaca ggcgctgctc gatcccgcgc
     4441 tcgccgatcc gaaccgcaag aacatcagtc tcgaaccgaa tggcggcgcg ctgcatggcg
     4501 tggccggcga cgccgtcacg caatcggcgg tgcgcgcgtt gatcgcgcgt tatcaggcca
     4561 atgcgcgcac gctcgtcgat gggctgttcc ctgaatacaa cggcaggttg cgcgtcgcgc
     4621 ccacgagcct gcggctgcat caggtggaag ggcgcgagac ctcgtggcgc aaggacgata
     4681 gccgtttgca tgtggacgca ttcccgtcgc ggccgaacta tggcgagcgc attctgcgcg
     4741 tgttcaccaa cgtcaatccg catggtgcgc cgcgcgtgtg gcgcgtcggc gaaccgttcg
     4801 aggatatggc gaggcgcttc ctgccgcgca tcaaaccgca aatgccgggt tcggcctggc
     4861 tgctgaactt gctgcatgtc accaaatcgc cgcgcagcga atatgaccat ctgatgctga
     4921 atctgcacga cagcatgaag gccgatctcg actatcagaa gtcgagcccg caggagacca
     4981 tgccgtttcc gccgggcagc gtgtgggtgt gtttctccga tcagacttcg catgcggtga
     5041 tgtccggcca gttcatgctg gagcagacct tcttcctgcc ggtcaaggcg atggcgcagc
     5101 ccgactgcgc gccgctcggc attctcgagc gcctgaaggg cagggcgctg gtttgagcgc
     5161 cgcgttcgct tgggattgcg cgcgcccgct cgacgcgaag ctcacggcga aagtcacatt
     5221 ggcacacgcg ctgaagccca gtttgaaacg cgcggagtct cgatgctgag ggcgatctat
     5281 aacgccctct ggtggatcat cgcgccgctt gccgtgttgc gtttgctgat ccgttcgcgc
     5341 aaggagcgtg gttaccgtga gcatatcggc gagcggttcg gttactcgcg cggccgcttg
     5401 ccggaagaca atgcgccgct gatctgggtg catgcggtgt cggtgggcga gacgcgtgcc
     5461 gcgcaaccgc tgatcgacgc gctgatgaaa gcgcgtcccg atgcgcgcgt tcttttgacg
     5521 catatgacgc cgagcggccg cgccaccggc gagcagattt tcggcgaccg cgtgttgcgc
     5581 agttatctcc cgtacgacat gccgcatgcg gtgcggcggt ttttacgggc atggcggccg
     5641 tcgctcggtc tggtgatgga aactgaagtg tggccgacct tgatcgacga atgccgtcgc
     5701 gcggatgtgc cgctggtgct gaccaatgcg cgcatgtcgg cacgctcgtt caagcgcgcg
     5761 gccaagttcg gccatgcgac cagggacgtg ttcggtgggt ttgcgcgcgt gctggcgcag
     5821 agtccgtcgg atgcggagcg gctcaccgca ctcggtgcgc gtaacgtcgc ggtgctcggg
     5881 aatctgaagt tcgatatgag cacgccggcg gaactggcgg cgcgcggtca tgcgtggcgc
     5941 gcggcgatcg gcacacggcc cgtgtgggtc gcggcgagca cgcgcgaagg tgaagaggaa
     6001 ctggtgctgc aggcgttcgc ggcgctgggc gtcgacgatg cgttgctgat tctggtgccg
     6061 cgtcatccgc aacggtttaa cgaagtggcg gcgttggcgg agaaggcgcg tttgcgtctt
     6121 gagcggcgct cgacgtgggc gcctgccgcg gcggtggcat cggcggctgc tgcagctgcg
     6181 ggcggtggcg tgccgccctt gccgtcagat gtgaaggtcc tgctcggtga ttcgatgggc
     6241 gagttgggcg cttactatgc cgcttcggat ctggcgttta tcggtgggag cttgctgccg
     6301 ctgggtgggc agaatctgat cgaggcatgc gcggtcggtg tgccggtatt gattgggccg
     6361 catgtgttca atttcacgca agccaccgcg gatgcggttg cggcgggggc tgcggtgcag
     6421 gtgcaagacc cggctgacct ggcccgtgtg cttcgcgaac tgttcagcga caaagcccgc
     6481 aggctggcaa tgggcggcgc ggcttcggcg tttgccgcgc gccatcgcgg cgcgacggca
     6541 cgcacggtgg atgtgttgat ggcggtgttg ccggagtgaa ggggaggcgc gaaagcgccc
     6601 cggctttaag gcctcaaagc ttggaagcgg gtctttcggc ctcaaacctt caacaacccc
     6661 ttctcctcga taaacttcac caccacatcc aagccgtcga gcgctttcag attacacatc
     6721 acgaaaggtc gctcgccgcg catcttcttc gcgtccgacg ccatcacctc caggttcgcg
     6781 ccgaccatcg gcgcgagatc ggtcttgttg atcaccagca gatccgactt cgtaatcccc
     6841 ggcccacctt tacgcggaat cttctcgccg ccggccacat cgatcacata gatcgtcagg
     6901 tccgacaact ccgggctgaa ggtcgccgcc aggttatcgc cgcccgattc gatgaacacg
     6961 atgtccgcat cgggaaaccg cgtcagcatg cggtcgacgg cttcgaggtt gatcgacgcg
     7021 tcttcacgaa tggccgtatg cgggcagccg cccgtttcca cgcccatgat ccgttcggcc
     7081 ggcaacgcgc ccgccacagt caagagacgc tggtcttctt tcgtatagat atcgttggtg
     7141 atcgcgacga ggtcgtactg ttcgcgcatc gccttgcaga gcatttcgag cagcgtggtc
     7201 ttgcccgaac caaccgggcc gccgacgccc acgcgcagcg gcggcagttt cttcgtgcgg
     7261 acagacgagt gagtggcttg atgaggtgcg ttcatgatgg atctttttac gagcggaaga
     7321 gccgcgaata ctgcgactcg tgacgcgccg acagaatgcc gagttgcggc gcgaaggtgt
     7381 tgatgttgtc gggcgaggtg gccaaggctc gtgtgacggc ggcatcgatc ggctcgcgta
     7441 gcgcgacgat gatgcgctgg ccggcgagtt ggccgagcgg cacggccttc aaggcggcgg
     7501 cggcctggtt ctccacccag ctgaatgcgt aggcggcgag cgcggcgtcg ggtgctgcgt
     7561 cgtgagcgaa agcggcgaag gcgaaagccg tgggttgggc caagggcttg agggaggaca
     7621 gcgtggcgcg tcgagcggcg tcgccccact ccagcgagac gcacagttgt cgcagtgacc
     7681 agcccatttg ctccgtctca cgcctgagtt cagcggactc acggcttgcc aggaattcgc
     7741 tgttcgcttc ctttaagcca tctgcgtcgt gctcacgcca gcgtttcatc tgatgtgcga
     7801 gaaacggcaa ctcgccgcgc gcaagcacat cggtcaagcc gcttgcgatc caatcgcgtg
     7861 cggaatcggc gtcggtgatg agttgcgctt cgatcgcggc ttcgagacct tgcgagtagc
     7921 tgaacgcgcc gatcggcaat gccggcgacg caagatgcag cagcgccgtg agttcagcga
     7981 tgcgcatggt ggtggtgatg cccgtggccg cacgattcgt cgtggacgtg atcgttagcg
     8041 ttgtggccgt gtgagtggtc gtggtcatgc gcgtgaccct gcgcgtggct atgaccgtgc
     8101 tcatgatccg gctggtcatg cccatggccc tgcgcatggc tgtgaccgtg ctcatgatcc
     8161 gaatgatcat gtccatgctc gtgatcatgc gagtgaccgt gtgcattgcc atgctcgtga
     8221 tcatgcgaat gctcatgccc atgatgctca ccgaacacct gctgcgcgat cgcatagtct
     8281 tcggcgaagg tctcatcgtg accatgcttg tgaccgccgc cgtacgcgcc ggattccggc
     8341 tggaacggca tcgacacctg atcgacactc gcgccgatgc gcttgagcat atcgcccagc
     8401 accggatcgt attcgagctt cagatagtcc gcgccgactt cgactggcgt gtgacgattg
     8461 ccgaggtggt aagcggcacg cgtcaacgtc aacgcgtctt tcgcacggac atacagcacc
     8521 gtttccggcg cggcgaccac gcgcaccagg ccgccatcgt cggcgaccag cacatcgccg
     8581 tcgcgcagca ccgtgccgcg cggcaacagc agcgcaacgt cctcgccgtt atcgagcgtc
     8641 gccgcgaggc ggctcttgcg gcggtcatcg aaagcgagcg tcaaggtcgg cgcgcgcttt
     8701 accaaaacgg gcgcgagctt cagatgcggt gcgatcagtt tgtcgatggt gcgcatggtt
     8761 tagaacagaa agtagcgttg cgccatcggc agcacggtgg cgggctcgca ggtcagcaac
     8821 tggccgtcgg cgatcacctg ataggtttcc ggatcgacgc tgatcgccgg acgccacgcg
     8881 ttgtgaatca tgtcggcctt cgtgatgttg cggcagttct tcacggcaac gatacgcttg
     8941 ttcagcccat agcgttcgcc gacgcctgca tcggcagcca tttgcgaaac gaaggtcagg
     9001 gacgtgcgtc ccagcgcacc gccgcgcgtc gcgaacatct cgcgataatg gaccggctgc
     9061 ggtgtaggaa tcgacgcgtt cgggtcgccc atctgcgcca tcgcgatcat tccgcccttc
     9121 aggatcaacg acggcttgat accgaaaaac gccggctccc agaacaccag gtcggcccat
     9181 ttacccggtt cgatcgagcc gacttcgtgc gagatgccgt gcgtgatcgc cgggttgatc
     9241 gtgtatttcg cgacataacg tttcgcgcgg aaattgtcgt gacgcgcgtt gtcttccggc
     9301 agcgcgccgc gttgcacttt catcttgtgc gcggtttgcc atgtgcggat gatgacttcg
     9361 cccacgcgcc ccatggcctg cgaatccgac gacagcatcg acaacgcgcc gagatcgtgc
     9421 aggatatctt cagccgcgat ggtctcgcga cgaatccgcg actcggcgaa cgcgatatct
     9481 tcggcaatcg acgggtccag gtgatggcac accatcagca tgtcgagatg ctcttcgagc
     9541 gtattgacgg tgtacgggcg tgtcggattg gtggacgagg gcagcacgtt cgcttcgccg
     9601 cataccttga tgatgtccgg tgcgtggccg ccgcctgcgc cttccgtgtg atacgtgtgg
     9661 atcgtgcgac ccttgaacgc agccacggtc gcttcgacaa atcccgcttc gttcaacgtg
     9721 tccgtgtgaa tcgcgacctg cgtgtcggta tcgtcggcga cggagagaca gttgtcgatt
     9781 gcggccggcg tcgtgcccca gtcttcgtgc agcttcaaac cgatcgcccc cgcggcgatc
     9841 tgttcaagcg cgggttgcgg ctggctcacg ttgcccttgc cgagaaagcc gagattcatc
     9901 ggataaccat cggcggcttg cagcatccgt tcgagatgcc acggccctgg cgtgcaagtc
     9961 gtcgcattcg tgccggttgc cggacccgtg ccgccgccga gcatcgttgt gacgccgctc
    10021 gcgagcgctt cttcgatctg ctgcgggctg atgaagtgga tgtgcgtatc gatgccgccc
    10081 gccgtcacga tcaagccctc gccggcgatc acttcagtgg atgcaccgat cgcgatcgtc
    10141 acgttcggtt gaatatcggg gttgcccgct ttgccgatcg cgaagatgcg gccgttcttg
    10201 atgccgatgt cggccttgac gatgccccag tgatcgagaa tcaccgcgtt cgtcacgacc
    10261 gtatcgacga cgtcggcgtg gacgcgttgc gactggccca tgccgtcgcg aatcactttg
    10321 ccgccgccga atttcacttc ttcgccgtag gtcgtgaagt cgcgttcgac ttcgatcagc
    10381 agctcggtgt cggcgaggcg tatgcggtcg ccggtggtgg ggccgaacat ttccgcgtat
    10441 gcgcggcggc caatgcgtaa ggtcatggtg tgagtccgga aatgagcagg gcggtcggtg
    10501 caagcgtgcg cgggtcgcgc atcagagctt gcccatcacc ttgccgttga agccatagac
    10561 gacgcgctcg cccgccagtt cgaccagttc gacggtacgt tcctggcctg gctcgaaacg
    10621 cacggcggtg cccgctgcga tgttcagccg gaagccgcgt gccgcttcgc gatcgaagga
    10681 gagcgcttcg ttcacttcgt agaagtgata gtgcgagccg atctgtaccg gacggtcgcc
    10741 ggtattcgac acgaccaccg tgacggtggc gcggcccgcg ttgagttcgt gttcgccgtc
    10801 gtcgatgagg agttcgccgg gaatcatggg gcgtgcctta cgtgattaaa gcgtgattag
    10861 ggaatcgggt gatgaacggt gacgagtttg gtgccgtcgg ggaacgtggc ttcgacctga
    10921 atgtcgggaa tcatttccgg cacgccttcc atcacgtcgg cgcgcgtcag cagcgtggtg
    10981 ccgtagtgca tgacctcggc gacggtcttg ccgtcacgcg cggcttccat cagcgcggcg
    11041 ctgatgaagg cgaccgcttc cggataattc agtttgaggc cgcgagcgcg gcgccgttcg
    11101 gcgagcagcg cggcggtgaa gatcagcagc ttgtccttct cgcgaggtgt cagcttcatt
    11161 gaatgtgtgc gtagggttgc gccggtccga gttgggccgg ctgcgacaat ggtaggcgct
    11221 gcgtgtcatg gactcatcgc gtgcggcgca cgggatgagt ccatgacgag tgcaatgccg
    11281 gataagccag ccacggtatc aagttccgct aacgaagcgc ttacaaatcg gtcggcagcg
    11341 gcgcgccgaa ccatttcttc gacatcgtgt tgagcgtgcc gtcctgtttg gccgccgcga
    11401 tcgcggcatt gaccttctgc tgcaggcgcg gctcgttctt gttcatgccg acgaagcacg
    11461 gcgaattctt gatgacgaac ttcggttccg gccgacgcgg cggatttttt gcgaggatcg
    11521 ccccggcgac gatattgccc gcggcgatca actgcacctg gccggacagg aacgcggcga
    11581 tggttgcgtt gttgtcttca aagcgtttga tggtggcgtt cggcgccatt tgcgtgagac
    11641 cgatttcctc gagcgcgccg cgcgtggcgc cgaccgtctt gccggtcaga tcggcgggac
    11701 cggtgacctt gatgtcggcg ggaccgaaca cgccctggta atacggcgca taggccgtcg
    11761 aaaaatcgat gactttgtcg cgctcaggcg ttttgccgag cgacgagatc accagatcga
    11821 ccttaccggt ttgcagatac ggaatgcggt tcgcgctgtt gaccggcacg agttcgagct
    11881 tcacattcat cgacttcgcg agcagggcgg ccgtgtcgat gtcgtagcct tgcggtttca
    11941 tatccgcgcc gaccgagcca aacggcggat agtcttcggg aaccgcgact ttcagcacgc
    12001 cggccttggc gatgttgtcg agcgtatcgg cgtgagccgt gggcgcagtg acggcgagga
    12061 cgggcgaaag cagcaatgcg gcaagccagg cgcggcgcgc ggggcgaagt gtcgatcggg
    12121 tcatgttatg tgagcggtga tggcgagaag cggtcggctg ccgtcaggac gcggcaacat
    12181 cgtggggaga ttgcgatgca aggtatgtgc catggcggtg cgacgtgggc tgacgcgacg
    12241 cgaaagaaga ctgtgcactg aattggagcg ccgcggtgct ccagactggt gcacgcggag
    12301 gcttacgaag gtgaaaggcg caacgactga cgtcgtaacg cgacaggagg aatgaccgca
    12361 ccgcgcgcgc agcgctgctg gaatgtgatc gatctttggc ggtcatacaa aacggtgaaa
    12421 cgcgttagag caatatcgat gctgcgtcag ccaaccgctg cagacgcagc aaccactaag
    12481 cgttacttcg cccagctatc agatataaaa gccgtagtcg gag
//
LOCUS       DATASH010000611         4010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_100224,
            whole genome shotgun sequence.
ACCESSION   DATASH010000611 DATASH010000000
VERSION     DATASH010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100224"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..980
                     /locus_tag="VHV99_16660"
     CDS             <1..980
                     /locus_tag="VHV99_16660"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007585905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HEX3637083.1"
                     /translation="LDRMEVIRLSGYTEDEKVSIAQRYLLPKQKKNNGLKDGEVDVTE
                     TAIRDIIRYYTREAGVRSLEREISKICRKVVKMLLLKKAEGAVKVDGSNIDTFLGVRK
                     YDFGLAAKENQVGQVTGLAWTEVGGDLLTIEAAVMPGKGNVIRTGSLGDVMKESVEAA
                     RSVVRSRSRRLGIKDEAFEKQDIHIHVPEGATPKDGPSAGIAMTTALVSVLTGIPVRA
                     DVAMTGEITLRGEVLPIGGLKEKLLAAHRGGIKLVLIPEENVKDLTEIPDNVKNAIEI
                     VPVRWVDKVLELALERVPQALPDEEPKPAAPVAAAEPGKDGAATDVVKH"
     gene            1332..1604
                     /locus_tag="VHV99_16665"
     CDS             1332..1604
                     /locus_tag="VHV99_16665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX3637084.1"
                     /translation="MNKTELIDHIAHEADISKAAAGRALDAVIGGVKGTLKKGGSVTL
                     VGFGTFAVGKRSARTGRNPRTGDAIKIKAAKVPKFRPGKALKDALN"
     gene            1749..1824
                     /locus_tag="VHV99_16670"
     tRNA            1749..1824
                     /locus_tag="VHV99_16670"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1782..1784,aa:Val,seq:tac)
     gene            1866..1942
                     /locus_tag="VHV99_16675"
     tRNA            1866..1942
                     /locus_tag="VHV99_16675"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1900..1902,aa:Asp,seq:gtc)
     gene            2029..3966
                     /locus_tag="VHV99_16680"
     CDS             2029..3966
                     /locus_tag="VHV99_16680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEX3637085.1"
                     /translation="MLDFFRNHKRLMMFMLILVIVPGLGFVGIQGFRGFFDESANVAS
                     VNGHKITRAEYDDAIRQQLDRARQMLGAQFDAKSFDTPERRREMLDGLIEQRVLADET
                     QRLHLTASDDAVRRVLLNDPVISSLKNPDGSIDVERYKQLLAMQGMTPDQYDERVRYS
                     LAMQQLPASIQGSTFTSKTLAQHLTELAEQQREVQGIAFHPRDYAAKVQPTDAQLHAY
                     YDAHRNEFATPATATIQYLVMSSATLSASIQPSDADLKKYYDDNIAHYRTDGEVRASH
                     ILISAPKDASSADKAKAKQKAEALLAQIKAHPDQFAQIAQANSQDPGSASKGGDLGYF
                     GRGMIAGGQAFDDAVFALKKDEVSGIVQTDFGFHIVKVTDVKPAVTKPFDEVKDQIAK
                     DLKTQLAGKAFSDSSEGFTSLVYEKAKSLQPAADKYKLQLQTATVTPQPDPKLPPDSP
                     LNNAKFLAAVFANDAAVQRNNTQAIDVGGNTLIAAHVTDYKPAAVPAFDAVKDAVRQK
                     VIAAQSNEAAHKDGVAKLAELEKSKATTGFSSPLKVSRNDAQGVPPAALSAIYKADAQ
                     KLPAYVGVDLGDDGYAIYRVNSVVAAAPADPQRLAAAQQQIAQVNAQSEAEAYVEALR
                     ARSKVKFYGSLDSGNSQTADQ"
BASE COUNT          887 a         1103 c         1263 g          757 t
ORIGIN      
        1 tgctcgaccg gatggaagtg atccgtctgt cgggttacac ggaagacgag aaggtcagca
       61 tcgcgcaacg ttatcttttg cccaagcaga agaagaacaa cggcctcaag gatggcgagg
      121 tcgatgtgac ggaaaccgcg atccgcgaca tcattcgtta ctacacgcgt gaagcgggcg
      181 tccgttcgct cgagcgtgaa atttcgaaga tctgccgcaa ggtcgtgaag atgcttctgc
      241 tgaagaaggc ggaaggcgcg gtcaaggtcg acggcagcaa tatcgacacc ttcctcggcg
      301 tgcgcaagta cgacttcggt ttggctgcga aggaaaatca ggttggccag gtcacgggtc
      361 ttgcgtggac ggaagtgggc ggcgatttgc tgaccattga agccgcggtg atgccgggta
      421 agggcaatgt gatccgtacc ggttcgctcg gcgacgtgat gaaggaatcc gtcgaagctg
      481 cgcgctcggt ggttcgctcg cgttcgcgcc gtctcggtat taaggacgaa gcgttcgaga
      541 agcaggacat tcacattcac gtgccggaag gcgctacgcc caaggacggt ccttcggcgg
      601 gtatcgcgat gacaaccgcg ctggtgtcgg tgttgaccgg tattccggtg cgggccgatg
      661 tcgcgatgac gggggaaatc acgttgcgtg gcgaagttct gccgatcggc ggcctgaagg
      721 aaaaactgct ggcagcgcat cgcggcggta tcaagctggt gctgattccg gaagaaaacg
      781 taaaggactt gacggagatt ccggacaacg tgaagaacgc aatcgaaatc gtgccggtcc
      841 ggtgggtcga caaggtgctc gagttggcgc tcgaacgtgt gcctcaggca ttgccggacg
      901 aagagccgaa gccggctgcg cctgtcgcag ccgctgagcc cggcaaggac ggcgcggcga
      961 cggacgtcgt gaagcactaa ggtagtcagc agcgaacgct gtttgctgta taaaaacccg
     1021 cgaccttggt cgcgggtttt ttattgtcgg cgagctaccg aagaatgcgt ttgaaacgca
     1081 aataatccca tttactgagc aatacacacg ttctgcgtcg ccgactgatc atgcgcgcat
     1141 cgattcgcgg caagatttgg ttatagaagt gccctcagaa tcttgcgaat caggctcaac
     1201 cccggttgac acaagggttt cggccaattc taatgaggcg gctcggcgtt gcccgcagcc
     1261 gcgtgatgtc aggcgctata gagcgtcgtc acgattgcca taatcagcca taaacattct
     1321 cgggggttgg aatgaataaa acggaattga tcgatcacat tgcacatgaa gccgatattt
     1381 cgaaggccgc ggcgggccgc gcattggatg cggtgatcgg tggcgtcaaa ggtacgttga
     1441 agaaaggcgg gtcggtgact ttggtgggtt tcggaacgtt tgccgtgggc aagcgttccg
     1501 cgcgcacggg acgcaatccg cgcacgggcg acgctatcaa gatcaaggca gcgaaggttc
     1561 ccaaatttag gcctggcaaa gctctgaagg atgcgctaaa ctaatgggct tgctgcttga
     1621 ggaattgcgg attcgctaaa gaagtgcggt gaatttggca tgacttggtg tcgcatcggt
     1681 tggatgcagt tgaggcacct agttgataaa tcaggcagca aacgcagaag cacactgaat
     1741 cttgaaacgg gtgcttagct cagttggtag agcggcgccc ttacaaggcg taggtcggga
     1801 gttcgagcct ctcagcaccc accagtttca gattcgttaa gtgtttcagc gcagttgcag
     1861 tataaggagt ggtagttcag tcggttagaa taccggcctg tcacgccggg ggtcgcgggt
     1921 tcgagtcccg tccactccgc cagtatcctc gaaaggcgaa ctccggttcg ccttttttat
     1981 tgcccgctgt tgtaagatcg ggcgttctgt ttttggcact cctcacgcat gctcgatttt
     2041 ttccgcaatc acaaacgcct gatgatgttc atgctcatcc tcgtcattgt gccggggttg
     2101 ggttttgtgg gtattcaggg ctttcgcggc ttctttgacg aaagtgcaaa cgtcgcgagc
     2161 gtcaacggtc acaagatcac gcgcgccgag tatgacgacg caatacgtca gcaactcgac
     2221 cgcgcccgtc agatgctcgg cgcgcagttc gacgcgaagt cgttcgatac gccggagcgc
     2281 cgtcgcgaaa tgctcgacgg tctgatcgaa cagcgcgtgc tggccgatga aacgcagcgc
     2341 ctgcatctga ccgcatccga cgacgccgtg cgccgtgtgc tgctcaacga tcccgtgatc
     2401 tcgtcgctga aaaatcccga cggctcgatc gacgtcgagc gctacaagca actgctcgcg
     2461 atgcagggca tgacgcccga ccagtacgac gaacgcgtgc gctacagcct tgcaatgcag
     2521 cagttgccgg caagcattca aggcagcacc ttcacgtcga agacgctcgc gcagcatctg
     2581 accgagcttg ccgagcagca gcgtgaagtg cagggcattg cgttccatcc gcgcgactat
     2641 gccgcgaagg tgcagccaac cgacgcgcaa ctgcacgcgt attacgatgc gcatcgcaac
     2701 gaattcgcga cgccggccac cgcaacgatc cagtacctcg tgatgtcgtc tgcaaccttg
     2761 agtgcgtcca tccagccgag cgacgcggat ctgaaaaagt actacgacga caacatcgcg
     2821 cactaccgca cggacggtga agtgcgtgcg agccacatcc tgatctccgc gccgaaagac
     2881 gcaagctccg ctgacaaggc caaggcgaaa cagaaggccg aggcgttgct cgcccagatc
     2941 aaggcgcatc cggatcagtt cgcgcagatc gcgcaggcga attcgcagga cccgggctcg
     3001 gcatcgaagg gtggcgatct ggggtatttc ggccgcggca tgatcgcggg cggccaggcg
     3061 ttcgacgacg ctgtgttcgc tctgaagaag gacgaagtca gcggcatcgt gcagaccgac
     3121 ttcggctttc acattgtcaa ggtgacggac gtgaagccgg cggtcacgaa gccgtttgac
     3181 gaagtgaaag atcagatcgc gaaagacctg aagacgcagt tggcaggcaa ggcattcagc
     3241 gacagttcgg aaggcttcac gtcgctcgtg tatgagaagg cgaagagcct gcaaccggcc
     3301 gccgacaagt acaagctgca actgcagacc gctacggtaa cgccgcagcc ggacccgaag
     3361 ctgccgcctg acagcccgct gaacaatgcg aagttccttg ccgcggtatt cgcgaatgac
     3421 gcggccgtgc aacgcaacaa cacgcaggca atcgacgtcg gcggcaacac gctgatcgcg
     3481 gcgcacgtca ccgactacaa gcccgcagcc gtgccggcgt tcgatgctgt gaaggacgcg
     3541 gtgcgtcaga aggtcatcgc ggcgcagtcg aacgaagccg cacataagga cggtgtggcg
     3601 aagctggccg agcttgaaaa gtcgaaggcc acgaccggtt tctcgtcgcc gttgaaggtg
     3661 tcgcgcaacg acgcgcaggg cgtgccgccg gctgcattga gcgcaatcta caaagcagat
     3721 gcgcagaaat tgccggccta cgtgggcgtc gacctcggcg acgacggcta tgcgatctat
     3781 cgcgtgaact cggtggtggc ggcagcgcct gccgatccgc agcgtctggc cgcggctcag
     3841 cagcagattg cgcaagtcaa tgcgcagtcc gaagccgagg cgtatgtcga agcattgcgt
     3901 gcccgttcga aggtgaagtt ttacggctcg ctcgacagcg gcaactcgca gacagccgat
     3961 caataaggcc gtcgccagaa ctcgcgggct acgttagccc gcacaaaaaa
//
LOCUS       DATASH010000612         5853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4651998,
            whole genome shotgun sequence.
ACCESSION   DATASH010000612 DATASH010000000
VERSION     DATASH010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5853
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4651998"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..157)
                     /locus_tag="VHV99_16685"
     CDS             complement(<1..157)
                     /locus_tag="VHV99_16685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637086.1"
                     /translation="MSHAMPDRLLPGSPYPLGASWDGLGVNFAVFSANAQKIELCLFD
                     PTGRKEIR"
     gene            complement(276..2486)
                     /gene="glgB"
                     /locus_tag="VHV99_16690"
     CDS             complement(276..2486)
                     /gene="glgB"
                     /locus_tag="VHV99_16690"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEX3637087.1"
                     /translation="MSEHDPAAGLQPLDIDALVEARHPDPFSQLGMHQTDAGPVVRAQ
                     LPNASHVTVIARADGSTLGELEQLRPGLFAGRITSAAPYRLRIDWHGTVQEIEDTYSF
                     GPVLGDEPLGRLAGGDPYAVLECLGSRPMEVDGVPGVRFAVWAPNARRVSVVGDFNAW
                     DGRRHPMRLRHQAGVWELFVPRVGPGTRYKYELLARDGHPLPLKADPCAMQTEKPPGT
                     ASVVAHVDEIEQFQWTDHEWIQARGARQTPRAPISIYEVHAESWLRVAEEGQRGLTWE
                     ELAERLIPYVKSMGFTHVEFMPIAEHPFGGSWGYQPLGQFAPSARFGKPEQFATFVDK
                     AHEAGVGVILDWVPAHFPNDAHGLIDFDGTPLYEHADPREGYHQDWNTMIYNLGRHEV
                     SAFLVASGLAWLKRYHVDGLRVDAVASMLYRDYSRAAGEWVPNIYGGRENLESIAFLK
                     RLNHEVGYVPGVPGAITIAEESTAWPGVTARVEDGGLGFQFKWNMGWMHDTLHYMQED
                     PVYRRYHHHNMTFGMVYAYSERFVLPLSHDEVVHGKGSLLGKMPGDRWQKFANLRAYF
                     GFMWTHPGKKLLFMGGEFGQMAEFDHDTSPHWHLLDDANHHGVQMLVRDLNHLYRKEP
                     ALYRLDSEPGGFGWLIGDDSSNSVFAYRRTDGAGQELVVVCNMTPVPRVGYRIGLPRS
                     GRWAEVLNTDAGVYGGSNMGNGGLIHTDAISSHGWPHSASLVLPPLATIVLRAE"
     gene            complement(2479..>5853)
                     /gene="treS"
                     /locus_tag="VHV99_16695"
     CDS             complement(2479..>5853)
                     /gene="treS"
                     /locus_tag="VHV99_16695"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HEX3637088.1"
                     /translation="GKPATLSDDPLWYKDAIIYQVHIKSFFDANNDGVGDFPGLIAKL
                     DYIAELGVNAIWLLPFYPSPRRDDGYDIADYRNVHPDYGQLTDVRRFIQEAHARGIRV
                     ITELVINHTSDQHPWFQRARRAKPGSNHRNYYVWSDTDQKYTETRIIFIDSEPSNWTH
                     DPMAGAYYWHRFYSHQPDLNFDNPAVLREVLQVMRFWLDMGIDGLRLDAVPYLVEREG
                     TNNENLPETHAVLKKIRATIDAEYPNRMLLAEANQWPEDVKEYFGDEDECHMAFHFPL
                     MPRIYMSIASEDRFPITDIMRQTPDLAETNQWAIFLRNHDELTLEMVTDSERDYLWNT
                     YASDRRARLNLGIRRRLAPLMERDRRRIELINSLLLSMPGTPVIYYGDELGMGDNIHL
                     GDRDGVRTPMQWSSDRNGGFSRADPEQLVLPPVMGALYGFDAVNVEAQSRDPHSLLNW
                     TRRMLATRRAKQTFGRGTIRFLKPENRKILAYLREMPDEPPILCVANLSRAPQAVELD
                     LSEFNGAVPIEMTADSVFPAIGQLTYLLTFPPYGFLWFLLCPGGQRPTWAQAHSEPLP
                     EFVTIVIREGQVGPTPENVRLLESEVLPSWLSRRRWFASKDQKMHAVRLAALTTIPNG
                     GFAFTEIEADVGDHTERYVVPIAITWGGETTFPLFMQLALARVRRGRNVGHLTDAFAL
                     PIFAHGVMRKLRERAAVPTVQKSEIRFIPTERFDELKDLGERPEIRWLAAEQSNSSLI
                     IADAAVLKLVRRLVSGIHPEAEISRYLTQLGYANTAPLYGEVVRVDPEGVPHTLCILQ
                     GFVDNQGDAWNWSLDYLRRSVDELAIAVDTEAQSAPDRDNEAILVEGYSALAGIIGRR
                     LGELHVALASPTDDPAFAPEPAGAEQVKAWVDGTQSMLASALDLLAPRIEQMSDPETK
                     ALAQSLIDRRAALVEVVGKLVSSDAGALRTRIHGDFHLGQVLVAQGDAYLIDFEGEPA
                     RTLEERRQKSSPLRDVAGLMRSLSYASAAAQSTTESAPQQTADRKRALFDRFRAHATA
                     TFLTQYRAAIADAPTPLVSPEAEQALLDLFLIEKAAYEIRYEAANRPTWLSLPVRGLA
                     ALTSRLLGDTGSPQHDPSTQAPGAATPPNPAEGDYE"
BASE COUNT         1050 a         1859 c         1878 g         1066 t
ORIGIN      
        1 ggcggatttc cttgcggccg gtggggtcga acaggcagag ctcgatcttt tgcgcgttcg
       61 ccgagaacac cgcgaagttc acgccgaggc catcccaact cgcaccgagc ggatacggcg
      121 agccaggcag aagccggtcg ggcattgcat gcgacatgat tccccttctc tgttctgatt
      181 tatagacgcc agcttgggtc ttcatgcagc gagcgcgcgg cgtagagtcc acgcagagcc
      241 catgcaggcc gcgcgctcgc gttacagcac gtcaatcact ctgcgcgcaa cacgatcgtc
      301 gcgagcggcg gcaataccag cgaagcggaa tgcggccagc cgtggcttga aattgcgtcg
      361 gtatgaatca gccctccgtt gcccatgttc gagccgccgt acacgccggc atccgtattc
      421 agcacttccg cccagcgtcc gctgcgcggc aggccgatgc gatagcccac acgcggcacc
      481 ggcgtcatgt tgcagaccac cacgagttcc tggccggcgc cgtcggtgcg ccgataggcg
      541 aacacgctgt tcgaactgtc gtcgccgatc agccagccga agccgcccgg ctcgctgtcg
      601 agccgataca gcgcgggctc tttgcggtac agatggttca ggtcgcgcac cagcatctgc
      661 acgccgtggt ggttcgcatc gtcgagcaga tgccaatgcg gcgaggtgtc gtgatcgaac
      721 tccgccatct ggccgaattc cccacccatg aacaagagct tcttgcccgg atgcgtccac
      781 atgaaaccga agtacgcgcg caggttggcg aacttctgcc agcggtcgcc gggcatcttg
      841 ccgagcagag agcctttgcc gtgcaccact tcgtcgtgcg aaagcggcag cacgaagcgc
      901 tccgaatacg cgtacaccat gccaaacgtc atgttgtgat ggtggtagcg gcgatagacc
      961 ggatcttcct gcatgtagtg cagcgtgtcg tgcatccagc ccatgttcca cttgaactgg
     1021 aagccgagac cgccgtcttc gacacgcgcc gtcacgcccg gccacgcggt cgattcttcg
     1081 gcgatcgtga tcgcgccggg cacgcccggc acataaccga cttcatgatt caggcgcttc
     1141 agaaacgcaa tcgattcgag attctcgcgg ccgccgtaga tgttcggcac ccactcgccg
     1201 gcggcgcgcg aatagtcgcg atacagcatc gaggcgactg catccacgcg caggccgtcg
     1261 acgtgatagc gcttcaacca cgcaaggccc gatgccacca ggaacgcact cacctcgtgg
     1321 cggccgaggt tgtagatcat cgtgttccag tcctggtgat agccttcgcg cggatcggcg
     1381 tgctcgtaga gcggcgtgcc gtcgaagtcg atcagaccgt gcgcgtcgtt cggaaagtgc
     1441 gccggcaccc agtcgagaat cacgccgaca ccggcctcgt gcgccttgtc gacgaacgtg
     1501 gcgaactgct cgggcttgcc gaagcgcgcc gagggcgcga attgacctaa cggctgatag
     1561 ccccacgagc cgccgaacgg gtgctcggca atcggcatga actctacatg cgtgaagccc
     1621 atgcttttga cgtaaggaat caggcgttcg gcgagttctt cccacgtgag gccgcgctgg
     1681 ccttcttcgg cgacgcgcag ccacgattcg gcgtgcactt catagatcga gatcggcgcg
     1741 cgcggtgttt gtctggcgcc gcgcgcttga atccattcgt gatcggtcca ttgaaactgc
     1801 tcgatctcgt cgacatgcgc gaccaccgat gcggtgcccg gcggtttttc cgtctgcatg
     1861 gcgcacggat cggccttgag tggcaacgga tgaccgtcgc gggcgagcaa ttcgtatttg
     1921 tagcgcgtgc cgggccccac gcgtgggacg aacaattccc acacccccgc ctgatgacgc
     1981 aagcgcatcg gatggcggcg gccatcccac gcgttgaaat cgccgaccac cgaaacgcgc
     2041 cgcgcattcg gcgcccagac ggcgaagcgc acgccgggca cgccgtccac ttccatcggc
     2101 cgcgagccga ggcattcgag caccgcgtac gggtcgccgc cggcgagacg cccgagcggt
     2161 tcgtcgccga gcacggggcc gaacgaatac gtgtcctcga tttcctgcac agtgccgtgc
     2221 cagtcgatac gcaagcggta cggcgccgcg gaagtgatgc ggccggcaaa cagccccggc
     2281 ctgagttgct cgagttcacc gagcgtcgaa ccgtcggcac gcgcaatgac ggtgacgtgc
     2341 gaggcattcg gcagctgcgc gcgcacgacc ggacccgcgt cggtctgatg catgccgagc
     2401 tgcgagaacg gatcggggtg gcgcgcttcg acgagcgcgt cgatatcgag cggttggagg
     2461 cctgcggccg gatcatgctc actcatagtc gccctcggcc ggattaggcg gtgtggcggc
     2521 gcctggcgcc tgggttgacg gatcgtgctg cgggctgccg gtgtcgccaa gcaaccggct
     2581 ggtgagcgcg gcgaggccgc gtaccggcaa actgagccac gtcggccggt tggccgcttc
     2641 gtaacggatt tcgtacgcgg ctttctcgat caggaagaga tcgagcaagg cctgttccgc
     2701 ctcgggcgac accagcggtg ttggtgcatc agcaatcgcg gcgcggtact gggtcaggaa
     2761 cgtcgcggtg gcatgcgcgc ggaaacggtc gaacagcgcg cgcttgcgat cggcggtttg
     2821 ctgcggcgcg ctttcggtgg tggactgcgc cgccgcgctt gcgtaggaca gcgagcgcat
     2881 caagccagcc acatcgcgca acggactcga cttctgacga cgctcctcaa gcgtgcgtgc
     2941 aggctcgcct tcgaagtcga tcaggtacgc atcgccctgt gcgaccagca cctggcccag
     3001 atggaagtcg ccgtgaatgc gcgtacgcaa cgcgcccgca tcgctcgaca ccagtttgcc
     3061 gaccacctcg acgagtgccg cgcgacggtc gatcaggctt tgcgccagcg ccttggtttc
     3121 cggatcgctc atctgctcga tgcgcggcgc gagcagatcg agcgcgctag ccagcatgga
     3181 ctgcgtgccg tcgacccagg ccttcacctg ttcggcgccg gcgggttccg gcgcgaatgc
     3241 cgggtcgtcc gtcggcgagg ccagcgcgac atgcagttca ccgagccgcc ggccgatgat
     3301 gccggccaac gcgctgtagc cttccaccag aatcgcttcg ttgtcacgat ccggcgcgga
     3361 ttgcgcttca gtatcgacag cgatcgcgag ttcgtccacc gagcggcgca gatagtcgag
     3421 cgaccagttc caggcgtcgc cctgattgtc gacaaagcct tgcaagatac agagtgtgtg
     3481 cggcacgcct tcgggatcga cgcgtaccac ctcaccatac agaggcgcgg tgttcgcgta
     3541 gccgagctgg gtcaaatagc ggctgatttc cgcttccgga tgaatgccgc tgaccagccg
     3601 gcgcaccagc ttcagcacgg cggcgtcggc aatgatcagc gagctgttgc tttgctcggc
     3661 cgccagccag cgaatctcag gccgttcacc gagatccttg agttcgtcga agcgttcggt
     3721 cgggatgaac ctgatttcgc tcttctgcac ggtcggcacc gcagcgcgtt cgcgcagctt
     3781 gcgcatcacg ccatgagcga agatcggcag cgcgaacgcg tccgtcaggt gaccgacgtt
     3841 gcggccgcgg cgcacgcgtg ccagcgccag ctgcatgaag agcgggaagg tggtttcgcc
     3901 gccccacgtg atcgcgatcg gcacaacata gcgttcggtg tgatcgccta cgtccgcctc
     3961 gatttcagtg aacgcgaagc cgccgttcgg aatcgtggtg agcgcggcga gacgcaccgc
     4021 gtgcatcttc tgatccttcg aggcaaacca gcggcgccgg ctcagccatg acggcagcac
     4081 ttcggattcg agcaggcgca cattctccgg tgtcgggccg acctgaccct cgcgaatcac
     4141 gatggtgacg aattccggca gcggctccga atgcgcctgc gcccatgtcg gacgctggcc
     4201 gcccgggcac aacaggaacc agaggaagcc gtacggcggg aaggtcagca ggtacgtgag
     4261 ctggccgatg gccggaaaca cggaatcggc ggtcatttcg atcggcaccg cgccgttgaa
     4321 ttcggacaga tcgagttcga ccgcttgcgg cgcgcgcgac agattcgcca cacacaggat
     4381 cggcggctcg tcgggcatct cgcgcaggta cgcgagaatc ttgcggtttt ccggcttcag
     4441 gaagcgaatc gtgccgcgcc cgaaggtttg cttcgcgcgg cgcgtggcga gcatgcgccg
     4501 ggtccagttc agcagcgaat gcgggtcgcg gctttgcgct tcgacattga ccgcatcgaa
     4561 gccgtataac gcccccatca ccggtggcag caccaattgc tcaggatcgg cgcgcgagaa
     4621 cccgccgttg cgatccgacg accattgcat cggcgtacgc acgccgtcgc ggtcgcccag
     4681 gtggatgttg tcgcccatgc cgagttcgtc gccgtaatag atcacgggcg tgccgggcat
     4741 cgacagcagc agtgaattga tcagctcgat gcggcggcgg tcgcgttcca tcagcggcgc
     4801 gaggcggcgg cgaatgccga gattcagacg cgcgcggcga tcactcgcgt acgtgttcca
     4861 caggtaatcg cgctcggaat ccgtgaccat ttcaagcgtc agttcatcgt gattgcgcag
     4921 gaaaatcgcc cactggttgg tttccgcgag gtccggggtc tggcgcatga tgtccgtgat
     4981 cgggaagcga tcctcgctcg cgatcgacat gtagatgcgc ggcatcagcg ggaagtggaa
     5041 cgccatgtgg cattcgtcct cgtcgccgaa atattccttc acgtcttccg gccactggtt
     5101 cgcttcggca agcagcatcc ggttcggata ttcggcgtcg atggtggcgc ggatcttctt
     5161 cagcaccgcg tgcgtttccg gcaggttttc gttgttggtg ccttcgcgtt cgaccagata
     5221 cggcaccgcg tcgagccgca gcccgtcgat acccatgtcg agccagaagc gcattacctg
     5281 cagcacctcc cttagcacgg ccggattgtc gaagttcaaa tcgggctggt gcgagtagaa
     5341 gcggtgccag tagtacgcgc ccgccatcgg atcatgggtc cagttcgacg gctccgaatc
     5401 gataaaaatg atccgcgttt ccgtgtactt ctgatccgta tccgaccaca cgtagtagtt
     5461 gcgatgattg gacccgggct tcgcgcgacg ggcacgctga aaccacgggt gctgatccga
     5521 cgtgtgattg ataaccagtt cggtaatcac gcgaatgccg cgcgcgtggg cttcctgaat
     5581 gaagcgcctg acgtccgtaa gctggccgta gtccggatgc acattgcgat agtcggcgat
     5641 gtcgtagccg tcatcgcgac gcggcgacgg atagaacggc agcagccaga tcgcattcac
     5701 accgagttcg gcaatgtagt cgagcttggc gatcaggccg ggaaaatcgc ccacgccgtc
     5761 gttattcgcg tcgaaaaacg acttgatgtg cacctgataa atgatcgcgt ctttgtacca
     5821 gagcggatcg tcgctcaagg tcgcgggctt gcc
//
LOCUS       DATASH010000613         2095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5640925,
            whole genome shotgun sequence.
ACCESSION   DATASH010000613 DATASH010000000
VERSION     DATASH010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5640925"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="VHV99_16700"
     CDS             complement(<1..618)
                     /locus_tag="VHV99_16700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA N-terminal domain-containing protein"
                     /protein_id="HEX3637089.1"
                     /translation="MNDFWQHCSALLERELTPQQYVTWIKPLAPVAFDAAANTLSIAA
                     PNRFKLDWVKSQFSGRIADMARDFWHAPVDVQFVLDPKAGMRAPAASSSPAPARPSSA
                     AGSMGGGQSAGSNSGGAAVDAAVGAVQSAQAARANGANSAMANLNANARAAAEHNANA
                     RAAAAEDADLDLPSLDANEAAAARRTWRPGQSASSNGNGENDSMYE"
     gene            1011..1145
                     /gene="rpmH"
                     /locus_tag="VHV99_16705"
     CDS             1011..1145
                     /gene="rpmH"
                     /locus_tag="VHV99_16705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX3637090.1"
                     /translation="MKRTYQPSVTRRKRTHGFRVRMKTAGGRKVINARRAKGRKRLAI
                     "
     gene            1171..1659
                     /gene="rnpA"
                     /locus_tag="VHV99_16710"
     CDS             1171..1659
                     /gene="rnpA"
                     /locus_tag="VHV99_16710"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEX3637091.1"
                     /translation="MPGSEACGSHVGTAGAQQLGSVPLRAQAAFPKAARLLKTDEFSS
                     VFRLRPWRRTAHFVVYGRPTGNDARLGLVIGKKYAPRAATRNLVRRLAREAFRLRRAE
                     FGGWDVLLRLHTRFDKKALPSASSPPLRALCRSEIEALLDKAAREIARREAPPAEAPK
                     TE"
     gene            1784..2014
                     /gene="yidD"
                     /locus_tag="VHV99_16715"
     CDS             1784..2014
                     /gene="yidD"
                     /locus_tag="VHV99_16715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007581797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX3637092.1"
                     /translation="MQTVLFALLRFYKVAVSPMLGNRCRFYPTCSDYAREAIQYHGAA
                     RGTYLAARRICRCHPFSAGGIDLVPPPTSEKR"
BASE COUNT          369 a          674 c          650 g          402 t
ORIGIN      
        1 ctcgtacatc gagtcgttct cgccgttgcc gttcgaactc gcgctttggc caggccgcca
       61 cgtccggcgt gcggccgcgg cttcattcgc gtcgaggctg ggcagatcga ggtcggcatc
      121 ttccgccgcc gccgcgcgcg cgtttgcatt gtgttcagcc gccgcgcgtg cattcgcgtt
      181 gaggttcgcc atcgcgctgt tcgcaccatt ggcgcgcgcg gcttgcgccg attgcacggc
      241 gccgacggcc gcatccacag cagcaccgcc gctattcgag ccagccgatt ggcccccgcc
      301 catcgagcca gccgcggaag acggacgcgc cggagccggc gacgagctcg cagccggcgc
      361 acgcataccg gctttcgggt ccagcacaaa ttgcacatcg accggggcgt gccagaaatc
      421 gcgggccata tccgcgatgc ggccggagaa ctggctcttg acccagtcga gcttgaagcg
      481 gttcggcgcg gcgatgctaa gcgtgttcgc agcggcgtcg aaggcgaccg gggccaacgg
      541 tttgatccac gtcacgtact gctggggcgt aagctcacgc tccagcaatg cggaacagtg
      601 ttgccagaaa tcgttcatcg agttgctgtc gttatggtgt gcacggcggt tcgccgctgc
      661 ccctgtcgcg tgcgcgcaac aaggctctca gcagccaggc gtagcggctc caggcgcttg
      721 tgccacaagg gtttgcgggg caagcgagcc ggagcggcgt gcaggatttt tgggaagtaa
      781 ggcgagattc taacgccaaa tcgtcggcga aagggagtta tccacaggga ccgatcaatc
      841 ggtgaagtcc tctcggcgcc aggccgaacc cggctaaact attgacggtc aacgaaaaac
      901 cggtttaaat agcgggttcc gcgaaaacca attcagtaaa cctccggcca ttgccgcttc
      961 agcgatgatc gtgcggttat ttttcgatca gtcgatcaag tgagagcaac atgaaacgta
     1021 cttaccaacc ttccgttacc cgtcgcaagc gcacccacgg cttccgcgtt cgcatgaaga
     1081 ccgctggtgg ccgcaaggtc atcaacgcac gtcgcgcgaa gggccgcaag cgcctcgcca
     1141 tctaaggtta gcggattggg cgctccgtct gtgccgggaa gcgaagcctg cggtagtcat
     1201 gtgggaacgg ccggggcgca gcaactgggc tcggttccgt tgcgagcgca agccgccttc
     1261 cccaaagccg caaggctact gaaaacggat gaattttcat ccgtttttcg tttgcgcccg
     1321 tggcgccgta ccgcgcactt cgtggtgtac ggccggccca ccggcaacga cgctcgctta
     1381 ggcctcgtaa tcggcaagaa gtatgcgccg cgcgcggcca cgcgtaatct ggtacgtcgg
     1441 ctcgcgcgtg aagccttccg gctgcgtcgc gcggaattcg gcggttggga tgtgttgctg
     1501 cgtctgcaca cgcgcttcga caagaaggct ttgccgagcg cctcgtcgcc cccgttgagg
     1561 gcgctgtgcc gcagcgagat agaggcgctg ctcgacaagg cagcgcgtga aattgcccgt
     1621 cgtgaggcgc cgcccgcgga agcgcccaag acggaatgac gctcaagtga ccgcccgatt
     1681 ggtcgtacgg gcagcgcggt tcgccgaaat tgcttcggca gccctgctgc acgggcctca
     1741 cccggtactc cacgttgcgc ggcgccgtcc ggccgcacca gccatgcaaa cggtactctt
     1801 cgctttattg cgtttctaca aggttgctgt gagccccatg ctcggcaacc ggtgccgttt
     1861 ttaccctacc tgctctgatt acgcgcgcga agcaatccag tatcatggcg ccgcgcgcgg
     1921 gacttatctc gccgccaggc gtatttgccg ctgccacccg ttttccgcgg gcggcatcga
     1981 tctcgtcccg cctcccactt ctgaaaagcg ctgacgcgcc gttccatcga cactgagaca
     2041 acgcatggat atcaaacgca ccgtcctatg ggtcatcttt ttcatgtcag ctgtc
//
LOCUS       DATASH010000614         5142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5046964,
            whole genome shotgun sequence.
ACCESSION   DATASH010000614 DATASH010000000
VERSION     DATASH010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5142
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5046964"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="VHV99_16720"
     CDS             complement(<1..753)
                     /locus_tag="VHV99_16720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018434647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HEX3637093.1"
                     /translation="MEPSSLAAQSLSDEDLRALRRAKHELESPALAMKLASIVGSPLE
                     KLLSRMPAFANEKVTDATELALRKCLSIALRTLGRQAGTSPLIVPDKPSNLLHKFAVA
                     TTGAAGGAFGLFALPVELPVTTTLMFRSICDVARSEGENLTSIDTQLQCLTVLGMGGT
                     SKADDDADFGYFIMRGALAQAVSKASSEIATKGFTAHGSAALLRLLNTIAARFSVQVS
                     EQIAAKSIPAIGAVLGAMVNTVFIDHFQQVAHG"
     gene            897..2492
                     /locus_tag="VHV99_16725"
     CDS             897..2492
                     /locus_tag="VHV99_16725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006406581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEX3637094.1"
                     /translation="MAVHTAAHHSSGQVLPFRESLLAMLGISFVTMLVALDQTVVGTA
                     LPTIVAELKGFELYAWVATSYLLTSVITVPIFGRLGDYYGRKPFVIASIVVFTGASVL
                     CGAANNMLFLVLARGLQGIGGGMLVGTAFACIPDLFPDSVVRLRWQVLMSSAFGIANA
                     VGPSLGGFLTQYYGWRSVFYVNLPVGLLSLFFVWRFLPHLRHVEHEGKMRLDWPGALL
                     IAVALGSLQLFVELLPKHGVTLSAFALLALSIASAYGAWQWEKRCPTAILPVDMFRNR
                     SLAALFTLAVLGGFTMFSLLFYAPLLFQGGFGMSPKEAGLVITPLVVFITIGSIANGR
                     IVSRVRNPNLMLYIGFVLLALACLGVVVATRSMPQWLLMSFMMVGGLGLGFVMPNLTI
                     FAQQTAGREHLGIATALMQSLRMIGGMIGTALTGTLVSHMYASGVRSMLENDHASQWF
                     PDLGDPQILINRDAQDTLLGQLTHAGHNGAMLLESARETLVAAIHLGLALAAIIAVVS
                     VWQSRRVPPIKLQRKIEPVIHAD"
     gene            2524..3024
                     /locus_tag="VHV99_16730"
     CDS             2524..3024
                     /locus_tag="VHV99_16730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX3637095.1"
                     /translation="MEEQDRVAVMQQFGRTYRTFMSAFEAQVGQPLPRWRILLALHEQ
                     AGESSQKRLVERLRVDPGALTRQLKTLEGLGWIARSMDTRDNRVTNVRLTEAGRSATE
                     ASLPRRNAFLHDTMAALPDDALSALSGALKMLEARIGEVAAMANASPVAAAAQAPDAG
                     GGDPAL"
     gene            complement(3228..4505)
                     /locus_tag="VHV99_16735"
     CDS             complement(3228..4505)
                     /locus_tag="VHV99_16735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid deaminase"
                     /protein_id="HEX3637096.1"
                     /translation="MKVTNYQGAAIDPYSKGLGMVPGASIQLTDAPRLEWNLLNEDVS
                     LPAAVLYADRVEHNLKWMQAFVAEYGVKLAPHGKTTMAPQLFRRQLETGAWGITLATA
                     HQVRAAYHGGVSRILMANQLVGRRNMMMVAELLSDPDFEFFCLVDSVEGVEQLGEFFK
                     SVNKQLQVLLELGVPGGRTGVRDEAQRNAVLEAIARYPNVLKLAGVELYEGVLKEEHE
                     VREFLQSAVAVTRALVEEGRFARTPAVLSGAGSAWYDVVAEEFAKASETGKVEVVLRP
                     GCYLTHDVGIYRKAQTDIFARNPVAKKMGEGLLPALQLWAYVQSIPEPDRAIIGLGKR
                     DSAFDAGMPEASRHYRPGTEAPRDIAASEGWEIFGLMDQHAYLRIPAGADLKVGDMIA
                     FDISHPCLTFDKWRQVLVVDPSYRVTEVIETFF"
     gene            4652..>5142
                     /locus_tag="VHV99_16740"
     CDS             4652..>5142
                     /locus_tag="VHV99_16740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEX3637097.1"
                     /translation="MNSTAEPLTFDIVARIAECAPELRSAERKVAALILDDLTGASRA
                     SIGALAQQAEVSVATVTRFAKAVGCRDVRELKLRLAQAAAVGQRFLQAGAATDAPEPI
                     ATRVFDEVQTALAHNHQLLRQAPFDEAAAALRAARMIYVFGMGGGSTALADEMRFRLV
                     RLGR"
BASE COUNT          882 a         1618 c         1616 g         1026 t
ORIGIN      
        1 gccgtgcgca acctgctgga agtggtcgat gaacaccgta ttgaccatcg cgccgagcac
       61 cgcgccgatc gccggaatcg acttggcggc gatctgctcg ctcacctgca ccgaaaagcg
      121 cgcggcaatc gtgttgagca ggcgcagcaa tgctgcggat ccatgcgcgg tgaagccttt
      181 cgtcgcgatt tcggatgaag ctttcgatac cgcctgcgcc agtgcgccgc gcatgatgaa
      241 gtagccgaag tcggcgtcgt cgtcggcttt cgaggtgccg cccatgccga gcaccgtcag
      301 gcactgcaac tgcgtgtcga tcgaggtcag attctcgccc tcgctgcgcg cgacgtcgca
      361 gatcgagcgg aacatcagcg tggtcgtgac cggtaactcg acgggcagcg cgaacaggcc
      421 gaatgcgccg ccggccgcac cggtggtcgc cacggcgaac ttgtgcaaca ggttgctcgg
      481 cttgtcgggc acgatcagcg gcgaggtgcc ggcctgccgc ccaagtgtgc gcagcgcgat
      541 cgagagacac ttgcgcaagg ctagctccgt tgcatccgtg accttttcat tagcaaaagc
      601 cggcatgcgc gagagcagct tctcgagcgg cgaaccgaca atgctggcca gtttcatggc
      661 cagcgcgggg ctctccagtt cgtgtttcgc acgtcgcagc gcgcgcaggt cttcatccga
      721 taacgattgt gctgcgagcg aactgggctc catgtgcctc cctggcttca tgtgttgtgt
      781 tcattcttca gtcgattgcc gacgcgatgg acccgcatcg gcggactcca agttagagcc
      841 tcgtctcgtg gtatgattcc cgatcgtttg acaagtcaac tgttgcgcta acaaaaatgg
      901 ctgtccatac cgcggcccac cattcgagtg ggcaagtttt acctttccgt gaatcgctgc
      961 tggcaatgct cggcatttcg ttcgtcacga tgctggtcgc cctcgaccag accgtggtcg
     1021 gcaccgcatt gcccacgatc gttgcggaac tgaagggttt cgagctttac gcgtgggtcg
     1081 ccacgtcgta tctgctgacc tcggtgatca ccgtgccgat cttcggccgg ctcggcgact
     1141 actacgggcg caagccgttc gtgatcgcct cgatcgtcgt gttcacaggg gcttcggtgc
     1201 tgtgcggcgc cgccaacaac atgctgttcc tcgtgctcgc gcgcggcttg cagggcatcg
     1261 gcggcggcat gctggtcggc accgccttcg cgtgcattcc tgatctgttt cccgactcgg
     1321 tcgtgcgact gcgctggcag gtgctgatga gttcggcgtt cgggattgcg aacgcggtgg
     1381 ggccgtcgct cggcggtttt ctcacgcagt actacggctg gcgctcggtg ttctatgtga
     1441 atctgccggt cggcttgttg tcgctgtttt tcgtctggcg ctttctgccg catctgcggc
     1501 atgtcgagca cgaaggcaag atgcggctcg actggccggg cgcgttgctg atcgcggtag
     1561 cgctcggctc gctgcaactg ttcgtcgagt tgctgccgaa gcacggcgtc acgttgagcg
     1621 ccttcgcgct gcttgcactg agcatcgcct cggcatacgg cgcgtggcag tgggaaaagc
     1681 gttgccccac ggcgatcctg ccggtcgata tgttccgcaa ccgcagtctg gcagcgctct
     1741 tcacgctcgc cgtgctgggc ggcttcacga tgttctcgtt gctgttctac gcaccgttgc
     1801 tgttccaggg cggcttcggg atgtcgccaa aagaagcggg gctcgtcatt acaccgctgg
     1861 tcgtgttcat cacgatcggc agtatcgcaa acggccgcat cgtctcgcgt gtgcgtaacc
     1921 ccaatctgat gctgtacatc ggtttcgtgt tgcttgcgct ggcgtgcctc ggcgtggtcg
     1981 tcgcgacgcg ctccatgccg caatggctgt tgatgtcgtt catgatggtc ggcggtctcg
     2041 gtctcggctt cgtgatgccg aatctgacga tcttcgcgca gcagacagcc ggccgcgaac
     2101 atctcggtat cgccactgcg ctgatgcagt cgctgcggat gatcggcggg atgatcggca
     2161 cggcactgac cggcacgctg gtcagccata tgtacgcgag cggcgtgcgc agcatgctgg
     2221 agaacgacca cgcgtcgcag tggttccccg atctcggcga cccgcaaatc ctgatcaatc
     2281 gcgacgcgca agacaccttg ctcggccaac tgacgcatgc gggccataac ggcgcgatgc
     2341 tgctcgagag tgcgcgcgag acactcgtcg cggcgattca tctgggtctc gcgctcgcgg
     2401 cgatcatcgc cgtggtatcc gtgtggcaaa gccgccgtgt gccgccgatc aagcttcagc
     2461 gcaagatcga gccggtgatt cacgcggatt gattttttag ccttaccgtt ttactgacag
     2521 atcatggaag aacaggaccg cgtcgccgtg atgcaacaat tcggccgcac ttatcggacg
     2581 ttcatgtcgg cgttcgaggc ccaggttggc cagccgctgc cgcgctggcg cattctgctc
     2641 gcgctgcacg agcaagccgg cgagtcgtcg cagaagcgcc tggtcgaacg tttgcgcgtc
     2701 gatccgggtg cgctcacgcg gcagttgaaa acgctggagg gcttaggctg gattgcgcgc
     2761 agcatggata cacgcgataa ccgcgtaacc aatgtgcgtt tgaccgaagc ggggaggtcg
     2821 gcgaccgaag ccagcctgcc gcgccgcaac gcatttctgc acgacacgat ggcagcgctg
     2881 ccggacgatg cgctgagcgc actgtcgggc gcgctgaaga tgctggaggc gcgaatcggt
     2941 gaagtggcgg cgatggctaa cgcttcgccc gtggccgctg ctgcgcaagc gcctgacgcg
     3001 ggcggcggtg acccggcgct ttagcaagcg cccgactcgg ctgccaatga tccggcgttc
     3061 tagcgcgtga cagacggaag cgcggccgat caaacgccgc ggccgccgca aaccgcacga
     3121 gccggcacga cctggcgcca cgactcacgc caaatctcat agacgtcgtg aaaggcgcgc
     3181 caccttgcga tgaagcgcgc cggggtgcat cacggtttgc atcaagttca gaagaacgtt
     3241 tcgatcactt ccgtaacgcg atacgacgga tcgaccacca gcacctgacg ccatttgtcg
     3301 aacgtcaggc acgggtgcga gatgtcgaac gcgatcatgt cgcccacctt cagatcggca
     3361 ccggcgggaa tccgcagata cgcgtgctgg tccatcaaac cgaaaatctc ccagccttcg
     3421 ctcgccgcga tatcgcgcgg cgcttcggtg cccggacgat aatggcgcga cgcttcaggc
     3481 atgcccgcgt cgaaagcgga atcgcgtttg ccgaggccga tgatcgcgcg atccggttcc
     3541 gggatcgact gcacatacgc ccacagttgc agagccggca acaggccttc gcccattttc
     3601 ttcgcgacgg gattgcgggc gaaaatgtcc gtctgcgctt tgcggtagat gccgacgtca
     3661 tgcgtcagat agcagccggg ccgcagcacc acttccacct tgccggtctc cgaggccttc
     3721 gcgaattctt ccgcgacgac gtcgtaccag gcggagcccg cgcctgacaa gacggccggc
     3781 gtgcgggcaa aacgtccttc ctcgacaagc gcgcgcgtca ccgcgacggc gctctgcaag
     3841 aactcgcgca cttcgtgctc ttccttgagc acgccctcat acaactcgac gcccgccagc
     3901 ttcaacacat tcgggtagcg cgcgatcgcc tcgagcaccg cgttgcgctg cgcttcgtca
     3961 cgtacgcccg tgcggccgcc cggtacgccg agttcgagca gcacttgcag ttgcttgttc
     4021 accgacttga aaaactcgcc caactgctcg acgccttcga ccgaatcgac gaggcagaag
     4081 aactcgaaat cgggatcgct cagcaattcg gcgaccatca tcatgttgcg gcggccaacc
     4141 agctgattgg ccatcaggat tcgcgatacg ccgccgtgat aggccgcgcg cacctgatgc
     4201 gcggtggcga gcgtgatgcc ccacgcgccg gtttccagct ggcggcggaa caactgcggc
     4261 gccatggtgg ttttgccgtg cggcgcgagc ttgacaccat attccgcgac gaatgcctgc
     4321 atccatttca ggttgtgttc cacccgatcc gcgtacagca cggcggcggg caggctcacg
     4381 tcttcgttca gcagattcca ctcgagacgc ggggcatccg tgagttggat actggcgccg
     4441 ggaaccatgc ccaagccctt gctataagga tcaatcgctg cgccctgata gtttgtaact
     4501 ttcatgtttc cctgctccat cgtccagtta aattgaatca aagttgactt tagtatgtta
     4561 ccgaaagtac gatggttgaa gaaatgttat ttagtaccac ggtttgccgg aggctgtttg
     4621 taagcgtccg cgcggcctcc cacagtctgc aatgaactca accgccgaac cgctgacctt
     4681 cgacatcgtt gcgcgcatcg ccgagtgcgc gccggaactg cgttcggccg aacgcaaggt
     4741 cgcggcactg attctcgacg atctgaccgg cgcgtcgcgc gccagcatcg gcgcgcttgc
     4801 gcagcaggcg gaggtgagcg ttgcgaccgt gacgcgcttt gccaaggcgg tgggttgccg
     4861 tgatgtgcgg gaattgaagt tgcggctcgc gcaggcggcc gccgtcggcc agcgcttcct
     4921 gcaagcgggc gcagctaccg acgcgcccga gcccattgcg actcgcgtgt tcgacgaagt
     4981 acagaccgcg cttgcccata accaccagtt gctacgccag gcgccgttcg acgaagcggc
     5041 ggccgcgttg cgcgccgcgc ggatgatcta cgtattcggc atgggcggcg gctcgaccgc
     5101 attggccgac gagatgcgct tcaggctcgt gcgtctgggc cg
//
LOCUS       DATASH010000615         7057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5837180,
            whole genome shotgun sequence.
ACCESSION   DATASH010000615 DATASH010000000
VERSION     DATASH010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7057
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5837180"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..1428)
                     /locus_tag="VHV99_16745"
     CDS             complement(154..1428)
                     /locus_tag="VHV99_16745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014752481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3637098.1"
                     /translation="MTRYKSDGTAAFDSTGNLAAERESFSSADSRRPARALIAAVSGY
                     AMDGFDLLILGFMLSLISTDLGLTSSQAGSLVTWTLVGGVLGGVGFGILSDYLGRVRV
                     LTYTILMFALFTGLCALATSYWDLLAYRFAAGVGLGGEFGIGMALAIEAWPADKRGRV
                     SSYVGMGWQAGVLAAAVVTPLLLPHIGWRGMFVVGLFPAVGSFIIRKAIGESELFVRK
                     AATVKREFPVKTLFRDATATRRSIGVIILCSVQNFGYYAMIVWMPAYLSKTFGYTLGK
                     SAVWTGCSIVGMLVGIWIFGQLADRVGRKPAFIGYQIGALIMVLIYSTLRSPEGLLIG
                     GIFMGVFVNGMMGGYGALMAELYPTHARSTAQNVLWSIGRAVGGFGPLAIGALAAHYS
                     FGTAIALLASIYIIDIAATLFLIPELRGVELE"
     gene            complement(1483..2430)
                     /locus_tag="VHV99_16750"
     CDS             complement(1483..2430)
                     /locus_tag="VHV99_16750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoyl-D-amino-acid hydrolase"
                     /protein_id="HEX3637099.1"
                     /translation="MSHARKLGLAVAQMGPVHLADSRAAVVARLVDMLREAKSRNADL
                     VVFPEMALTTFFPRYWMSEEEAVDRFFERSMPNAHVQPLFDAARELGIGFYLGYAEIT
                     PEGQRFNTAVLVDKAAKVVGKYRKIHLPGHSDHKPDAPFQHLEKKFFEVGDRGIPVFE
                     AFGTKVGMCLCNDRRWPETWRVMSLQSAELGVLGYNTPSWNIHWNEPPHLRMHTHLIS
                     LQASAYQNAMWIGAAAKCGAEDGFHMIGGSVIVAPSGEIVAQSASESDEVIFANVDLA
                     LGDTFREHVFNFAKHRRPEHYGLIVERTGAGAPLPYNKD"
     gene            complement(2470..3942)
                     /gene="hydA"
                     /locus_tag="VHV99_16755"
     CDS             complement(2470..3942)
                     /gene="hydA"
                     /locus_tag="VHV99_16755"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HEX3637100.1"
                     /translation="MNDFDLTIRGGTVASDVDTFRADVGIVGDRIVAVENDLPAGRRD
                     IDATGCYVLPGGIDSHCHVEQRSGMGRMGADDWYTASVCAAFGGTTTIVPFAAQHRGM
                     ALAQVAADYAQLAREKSVIDYSYHMILSETDSQTLNTDLPELIRSGITSFKVYMTYEQ
                     LKIDDYQMLDVLAVAARERALVMVHAENNDVIKWVSQRLLERGYTAPKFHGTSHVQLA
                     ESEATHRVIQLSRLFDVPILIVHVSAREALATIQAAQKMGAQVYGETCPHYLLLTEDD
                     MDLPGTEGAKYCCSPPLRDADAQQALWEALKQGTLQTLSSDHAPYRYDASGKLPKGDA
                     TTFKEVANGMPGLETRLPLMFSEGVGKKRLTLPEFVALSATNHARMYGLFPRKGLLQP
                     GADADVAIWNPLREVTLSAATLHDNVGYTPYEGMRVTGWPETVVSAGRVIVERGELHA
                     ERGSGRFIPRTTPMPCATERELTPSTRFFRSLSYGAHEQK"
     gene            complement(3935..4684)
                     /locus_tag="VHV99_16760"
     CDS             complement(3935..4684)
                     /locus_tag="VHV99_16760"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HEX3637101.1"
                     /translation="MSHTASALPTLGVLGGMGPLATVDFMHKVIDATPARHDQEHIPL
                     IVHSVPQIPDRTTAFLTGSDAPWGYLLAGLRVLENAGADVIAIPCNTAHLWHGRLAAS
                     ARVPVLHIGHAACDAIGRRADAVRRVGLLATSATVQARIYHSELERAGIEVVMPSASR
                     QETQVMAGIHAVKAGQLEQGRLLLRSAAEALADSHVDALLLACTEIPVALAGLSFGVP
                     VLDATDMLARECVAWWQRALAQSPSITPAHE"
     gene            complement(4681..5352)
                     /locus_tag="VHV99_16765"
     CDS             complement(4681..5352)
                     /locus_tag="VHV99_16765"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEX3637102.1"
                     /translation="MPQPICVINPNSLQNVTASIARAVEPLAHGGAGSLQCVTLDGAP
                     PGIVSQRDADRAAPLVAAFVEQHEARFSAFVLACYSDPGLFAAREVTHKPVIGIGQAG
                     IATASLLGERIGVIAISAAGIARHWRYYRALGVERKIAGERSIDLSVAESGDETLALE
                     RMNETARTLRDVDGADVIVLGCAGMAALRATIEAASGLPVVDPCTAAVSFAIARVRDT
                     AEGRS"
     gene            5549..6439
                     /locus_tag="VHV99_16770"
     CDS             5549..6439
                     /locus_tag="VHV99_16770"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX3637103.1"
                     /translation="MSLRQIEVFRAIMITGSVSEAARMLFVAQPSVSRVLSVTEERLG
                     LLLFERARGRLYPTPEAKQIFEEVEAAYAGVQRVDDLVRALVEGRSGKLNVVCSPSLG
                     VHLVPRAIARFNRQYPELPVHFEPLTHNNLVPRVMFGKNYVGVSMFEVVHPNLATEPL
                     VTVPLMCVSPRGWLPDSKTVSLRDLKGRPWIDYGHETPLGRVVGTAFGSEIRPRPTVE
                     VRSAMSACSLVEEGVGVALVDPLCLDATLRERIDVRPLRPEIALKVQAVYSVSEPLSH
                     PAKVFLEVLRNVLEEVPGNR"
     gene            complement(6517..>7057)
                     /locus_tag="VHV99_16775"
     CDS             complement(6517..>7057)
                     /locus_tag="VHV99_16775"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HEX3637104.1"
                     /translation="GLLVSHGQVVSPLTTDPTQSEAVLAITRQGRAYIAEAPQISAQD
                     LKLIDTAVSGSAILLKNGQDLSAQSPNEGDPLDPNPRTLVGLSQQGRFLYFVVIDGRV
                     AGYSTGTTNAQSAQLMKAIGADDAINLDGGGSTELVRADQLGMPFIVNTPSGGAERLD
                     ASAIGVHALKLPALPGLPF"
BASE COUNT         1298 a         2197 c         2308 g         1254 t
ORIGIN      
        1 cgccgtgaaa aggcggcgcc cacatgccgc gcacggagcc ggccgctgag cttcgaggtg
       61 tcgcgcaatt gcaggccacc gcgcctaccc gtattgcggt ggccgcctcg tcggcccgcg
      121 cagaaattca cgggccgccc cgcacgtcat ggctcattcg agttccacgc cgcgcaactc
      181 gggaatcagg aacagcgtgg ccgcgatatc gatgatgtag atgctggcga gcagcgcgat
      241 tgcagtaccg aacgaatagt gcgccgccag cgcaccgatg gcgagcggcc cgaaaccgcc
      301 caccgcacgg ccgatgctcc acagcacgtt ttgcgcggtg ctgcgggcat gagttgggta
      361 caactcggcc atcagcgcgc catagccacc catcatgccg ttgacgaaca cccccatgaa
      421 aatgccgccg attagcagtc cctcaggcga tctgagggtc gaataaatca agaccatgat
      481 gagcgcaccg atctgatagc cgataaaggc cggtttgcgt cccacacgat ccgcaagctg
      541 tccgaagatc cagatgccga ccaacatgcc gacaatcgaa cagcctgtcc agacggcgga
      601 tttccccagc gtgtagccga acgtcttcga cagatacgca ggcatccaga cgatcatcgc
      661 gtagtaaccg aagttttgca ccgagcacaa aatgatgacg ccgatactgc ggcgcgtggc
      721 ggtggcatcg cgaaacagcg tcttgaccgg gaactcacgc ttgaccgtgg ccgccttgcg
      781 cacgaacagt tccgattcgc cgatggcctt gcggatgatg aatgaaccga cagcagggaa
      841 gaggccgacc acgaacatgc cgcgccagcc gatatgcggc agcaacaggg gcgtgacgac
      901 agccgctgcc agcacgccgg cttgccagcc catgccgaca tacgacgaga ctcggccgcg
      961 cttgtcggcc ggccacgctt cgatggcaag cgccatgccg ataccgaatt cgccacccag
     1021 tcccacgccg gcagcgaagc ggtaggcgag aagatcccag tagctggtgg caagcgcgca
     1081 caaaccggtg aagagcgcga acatcagaat ggtataggtc agaacccgca cgcggccgag
     1141 ataatcgctg agaatgccga agccgacgcc acccagtacg ccgcccacca gcgtccacgt
     1201 gacgagcgag ccggcttgcg aactcgtcag cccgaggtcg gtggagatga ggctgagcat
     1261 gaagccgagg atcagcagat cgaagccgtc catcgcataa ccggagaccg cggcgattag
     1321 cgcgcgcgcg ggacgccgcg agtcggcgga cgaaaacgat tcgcgctccg cggcgaggtt
     1381 gccggtcgag tcgaatgcgg ctgtgccgtc tgatttatag cgagtcatga tagaggtccc
     1441 ctgaggtggc gaatgcccgg cgcgtgctgg atgggcgacg cgttagtcct tgttgtatgg
     1501 cagcggcgcg ccggcgccgg tgcgttcgac gatcagaccg tagtgttccg gccggcggtg
     1561 tttggcaaag ttgaagacgt gttcgcggaa ggtgtcgccc aatgcgaggt cgacgttcgc
     1621 gaagatcact tcatcgcttt cgctcgcgct ctgcgccacg atctcgcccg agggcgccac
     1681 gatcaccgat ccgccgatca tgtggaagcc gtcttccgca ccgcacttcg ccgcggcgcc
     1741 gatccacatc gcgttctgat aggcgctggc ctgcagggaa atcagatgcg tgtgcatgcg
     1801 caggtgcggc ggctcgttcc aatgaatgtt ccaggacggc gtgttgtagc cgagcacgcc
     1861 gagctcggcg ctctgcaacg acatcacgcg ccaggtttcc ggccaacggc gatcgttgca
     1921 caggcacatg ccgaccttcg tgccgaatgc ttcgaagacg ggaatgcccc ggtcacccac
     1981 ttcgaaaaac ttcttctcga gatgctggaa cggcgcgtcg ggcttgtggt ccgagtggcc
     2041 gggtaaatgg atcttgcggt atttgccgac caccttcgca gccttgtcga ccagcaccgc
     2101 ggtattgaag cgctgtccct ccggcgtgat ttccgcatag ccgagataaa agccgatccc
     2161 gagttcgcgt gccgcgtcga agagcggctg cacatgagca ttcggcatgg agcgctcgaa
     2221 gaaacggtcg acggcttctt cctcgctcat ccagtaacgc gggaagaacg tggtgagcgc
     2281 catttcgggg aacaccacca ggtcggcgtt gcggcttttc gcttcgcgca acatgtcgac
     2341 gaggcgcgcg acgacagcgg cgcgactgtc tgccagatgg accggtccca tttgtgcgac
     2401 ggcgagaccg agtttgcgag catggctcat gagtacctct tgctgaaaat gaatgggatg
     2461 ggcggtgttt tacttctgct cgtgtgcgcc gtagctgagc gagcggaaaa agcgggtcga
     2521 gggcgtgagc tcgcgttcgg tcgcgcacgg catcggcgtc gtgcgcggga tgaagcgtcc
     2581 gctgccgcgc tcggcgtgca actcgccgcg ttcgacgatc acgcgcccgg cactgaccac
     2641 cgtctccggc cagccggtca cgcgcatgcc ttcgtatggc gtatagccga cgttgtcgtg
     2701 cagcgtggcg gcgctcagcg tgacttcacg cagcgggttc cagatcgcga cgtcggcgtc
     2761 cgcgccgggc tgcagcagcc ccttgcgcgg gaatagccca tacatgcgcg catgattggt
     2821 ggcacttagc gcgacgaatt cgggcagcgt cagccgcttc ttgccgacac cttcggaaaa
     2881 catcagcggc aagcgcgttt cgaggccggg catgccgttg gcgacctcct tgaaggtggt
     2941 ggcatcgcct ttcggcaatt tgccgctggc gtcgtagcga tacggcgcgt gatccgacga
     3001 gagcgtctgc aaggtgcctt gttttaacgc ttcccagagc gcctgctgcg catcggcatc
     3061 tcgcagcggc gggctgcagc aatatttggc gccctcggtg ccgggcaggt ccatgtcgtc
     3121 ttcggtgagc agcagatagt gcggacaggt ctcgccatac acttgcgcgc ccatcttctg
     3181 cgcggcctgg atggtggcga gcgcctcgcg cgcggacaca tgcacgatca gaatcggcac
     3241 gtcgaacagg cgcgacaact ggatcacacg gtgcgtggct tcggactccg cgagctgtac
     3301 atggctcgtg ccgtgaaact tcggggcggt gtagccgcgc tccagcaagc gctgcgacac
     3361 ccatttgatg acgtcgttgt tctccgcatg caccatcacg agcgcgcgtt cgcgagcggc
     3421 caccgcgagc acgtcgagca tctggtagtc gtcgatcttc aactgctcgt aagtcatgta
     3481 gaccttgaac gacgtgatgc ccgagcggat cagttccggc agatccgtgt tcagggtttg
     3541 cgagtccgtc tcggacagga tcatgtgata gctatagtcg atcaccgatt tttcgcgggc
     3601 gagctgggca tagtccgcgg ccacctgagc gagcgccatg ccgcgatgct gggcggcgaa
     3661 cggcacgatc gtggtggtgc cgccgaaggc ggcgcaaacg ctggccgtgt accagtcgtc
     3721 cgcgcccatg cggcccatgc cggaacgttg ctcgacatgg cagtgcgagt cgatgccgcc
     3781 tggcaggaca tagcagccgg tcgcgtcgat atcgcgccgg ccggcgggca ggtcgttttc
     3841 cacggcgacg atgcgatcgc cgacgatgcc tacatcggcc cggaaagtat ccacgtcgct
     3901 ggcgaccgtg ccgccgcgta tcgtcaaatc aaaatcattc atgtgcggga gtgattgaag
     3961 gtgattgggc tagcgctcgt tgccaccacg cgacgcattc gcgcgccaac atgtccgtcg
     4021 cgtcgagaac gggcacaccg aagctcaggc cggcgagcgc aacggggatt tcagtgcagg
     4081 ccagcagcag ggcgtcgacg tgactgtcgg cgagcgcttc ggccgcgctg cgcagcagca
     4141 ggcggccttg ttcgagctgg ccggctttga ccgcatgaat accggccatc acctgtgtct
     4201 cctgacggct cgcggatggc atcacgactt caatgccggc gcgctcgagt tcgctgtgat
     4261 agatacgagc ctgcacagtg gctgaggtag cgagcaggcc gacgcggcgt acggcatcgg
     4321 cgcggcggcc aatggcatcg caggctgcgt ggccgatatg cagcacgggc acgcgcgccg
     4381 atgccgcgag ccggccatgc cacagatggg cggtattgca tggaatcgcg atgacgtcgg
     4441 cccccgcgtt ttccagcacg cgcaagccgg cgagcaggta gccccagggc gcgtcggagc
     4501 ccgtcagaaa cgcggtagtg cgatcgggta tctgcggaac gctatggacg attaagggga
     4561 tatgttcctg atcgtggcgg gccggcgtgg cgtcgatcac cttgtgcatg aagtcgaccg
     4621 ttgccagcgg acccataccg cccagtacgc cgagcgtcgg caaggcggat gcggtgtgac
     4681 tcatgagcgc ccctcagccg tgtcccgtac acgggcgatc gcgaagctca ccgccgccgt
     4741 acacgggtcg accacgggca ggcccgaggc ggcttcgatc gttgcgcgca acgcggccat
     4801 gcccgcgcag ccgagtacga tgacatccgc gccgtccaca tcgcgcagcg ttcgcgccgt
     4861 ctcgttcatg cgttcgaggg cgagcgtttc gtcgcccgat tcggcgacgg acaggtcgat
     4921 cgagcgttcg ccggcaatct tccgttccac gcccagcgcg cggtagtagc gccaatggcg
     4981 ggcgatgccg gccgcgctga tcgcgatcac gccgatcctc tcgccgagca gcgacgccgt
     5041 ggcgatgcca gcctggccga tgccgatgac cggcttgtgg gtgacttccc gcgccgcgaa
     5101 gaggcccgga tcgctatagc aggcgagaac gaacgcgctg aagcgggctt cgtgctgttc
     5161 gacgaaagcc gcgacgagcg gcgcagcgcg atcggcatcg cgctgcgaca cgatgcccgg
     5221 tggcgcccca tccagcgtga cacactgcag cgagccggcg ccgccgtggg cgagtggctc
     5281 cacggcccgt gcaatgctcg ccgttacgtt ctgaagcgaa ttcggattga tgacgcagat
     5341 cggctgtggc aagggaaacc ttcctgagtg atatccatgt cggtacccga accgtatcaa
     5401 tcgcccgccc ggacgagaaa tagttcaaaa ttggcagggt atagacgcaa tgcataccca
     5461 gcgctttgat gccttaccac cactgtggcg acaaccgtcc ccgaaggaag acatgcccgt
     5521 tgaagagtcc ccgcagagca ggctgagcat gagcctgcgc cagatcgagg tgtttcgcgc
     5581 catcatgatc accggctcgg tcagcgaggc cgcgcgcatg ctgttcgtcg cgcagccgtc
     5641 cgtgagccgg gtcttgtcgg tgaccgaaga gcggctcggc ttgctgttgt tcgagcgcgc
     5701 acgtgggcgt ctttatccga caccggaggc gaagcagatc ttcgaggagg tggaggcggc
     5761 ttatgctggg gtacagcgcg ttgacgacct cgtgcgagcg ctggtggagg gccgcagcgg
     5821 caagctgaac gtggtctgca gtccgagcct cggcgtgcac ctggtgccgc gtgcgatcgc
     5881 gcgattcaac cggcaatatc ccgagttgcc ggtgcatttc gaaccgctca cccataacaa
     5941 cctcgtgccg cgggtgatgt tcggaaaaaa ttatgtgggc gtgtcgatgt tcgaggtcgt
     6001 gcatcccaat ctcgccacgg aaccgttggt aacggtgcca ctcatgtgtg tgagtccccg
     6061 tggctggctg cccgacagca agacggtgag tctgcgcgat ctcaagggtc ggccgtggat
     6121 cgactatggc cacgagaccc cgcttgggcg cgtagtgggc accgcattcg gcagtgagat
     6181 ccggccgcgg cccactgtcg aagtgcgctc ggccatgagc gcgtgttcgc tggtggaaga
     6241 aggggtcggc gttgcgctgg tcgatccgct gtgcctcgac gccacgctgc gcgagcgtat
     6301 cgacgtgcgg cccttgcgcc ccgaaatagc gctcaaggtg caggccgtgt attccgtatc
     6361 ggagccgtta tcgcatccgg caaaagtttt cctcgaggtg ctgcgcaacg tgctcgaaga
     6421 agtgccgggg aatcgctgag ggaggtgccg gcccgggcgg cgcaattccg cccggtcctt
     6481 ttaaagaaat tgccgcatct tccggcgttc aacgcattaa aacggcaacc ccggcaatgc
     6541 cggcagcttc aaggcgtgca cgccgatcgc cgacgcgtcg agtctctccg caccgccgct
     6601 cggcgtattg acgatgaaag gcatcccgag ttgatcggcg cgcacgagtt cggtggagcc
     6661 gccgccatcg agattgatcg catcatccgc gccgattgct ttcatcagtt gcgccgattg
     6721 tgcattggtc gtgcccgtcg aatatcccgc gacgcgccca tcgattacga cgaaatagag
     6781 aaagcgcccc tgttgcgaca agccgaccag cgtgcgcgga ttcggatcga gcggatcgcc
     6841 ttcgttcggg ctttgtgcgc tgaggtcctg gccatttttt agcagaatgg ccgaacccga
     6901 gaccgccgta tcgatcagct tcagatcctg cgccgaaatt tgcggcgctt cggcgatata
     6961 ggcgcggccc tgccgtgtga tcgcgagcac ggcttcgctt tgcgtcgggt cagtggtcag
     7021 cgggctgaca acctggccgt gcgacaccag caagccg
//
LOCUS       DATASH010000616        10953 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2278133,
            whole genome shotgun sequence.
ACCESSION   DATASH010000616 DATASH010000000
VERSION     DATASH010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 10953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10953
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2278133"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1249
                     /locus_tag="VHV99_16780"
     CDS             <1..1249
                     /locus_tag="VHV99_16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HEX3637105.1"
                     /translation="ELTLGLIGAGAVVVGGVVVYNAWQGAKVRRKMPRPMPADTAESF
                     ARDDQEEQSPFIEPARPTTRREPVVGSDAAGETAAARVEPTFGATAAPLDTPADIQAE
                     TTTPNGYPEAEGIAEAEGGHAAESDELAEPILPAATTISSAPPAIVDRRIDCIVPIRL
                     NGPVAGDKVIPLAQRLRRAGSKPVHIEGKLEGGAWELLQNGARYEELRAAAQLANRSG
                     ALNELEFSEFVTGVQQFADALDASPEFPDMLETVAMACELDGFAAQCDAQLSINVLSD
                     GAPWSANYVQAVASQDGLLLSRDGTRFVKLDAKQSPVFMLQFGDTNFLRDDLTYKGGQ
                     MITLVLDVPVADEDILPFRLMCDYAKSLAERIGGRVVDDGRRPLPETALLAIEKQLMT
                     LYARLEQAGIPAGSPATRRLFSQ"
     gene            1415..3481
                     /gene="ligA"
                     /locus_tag="VHV99_16785"
     CDS             1415..3481
                     /gene="ligA"
                     /locus_tag="VHV99_16785"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX3637106.1"
                     /translation="MARTPASNLATSAPAERAAWLRAELERANYAYYVLDQPDLPDAE
                     YDTLFKELERIETEHPDLIVADSPTQRVGGEASSGFEPVVHDQPMLSLNNGFADEDIV
                     AFDKRVADALGKNASEPPAPVDYAAELKFDGLAISLRYVDGMFVQASTRGDGATGENV
                     TENVRTIRSIPLKLKGKRVPRVLDVRGEVLMFKRDFERLNERQRAAEQREFANPRNAA
                     AGSLRQLDSKITAQRPLSFFAYGIGVLEGIGMPATHSELLDWYKELGLPVNGERAVVQ
                     GAEGLLGFFHKVGEKRDKLPYDIDGVVYKVNRRDEQDALGFVSRAPRFALAHKFPAQE
                     ALTRLVAIDVQVGRTGAITPVARLEPVFVGGATVTNATLHNEDEVRRKDIRIGDTVIV
                     RRAGDVIPEVVSALLERRPEDAREFVMPTQCPVCGSSIERLPDEAIARCTGGLFCPAQ
                     RKQALWHFAQRRALDIDGLGEKIIDQLVEQNLVRTPADLFNVGFATLAELDRFAEKSA
                     QNLLDSLEKAKHTTLARFIYALGIRHVGESTAKDLAKHFGSLNPIMDASVEELLEVND
                     VGPVVAESIHQFFAEEHNRTVIEQLRAPGKVTWPEGPPAPKAPQGVLAGKTVVLTGTL
                     PTFAREEAKEMLEAAGAKVAGSVSKKTDYVVAGAEAGSKLAKAEELGIPVLDEDGMRK
                     LLEGQS"
     gene            3478..4011
                     /gene="def"
                     /locus_tag="VHV99_16790"
     CDS             3478..4011
                     /gene="def"
                     /locus_tag="VHV99_16790"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX3637107.1"
                     /translation="MIREILKMGDPRLLQIADPVDHFDTPELHELVKDMFETMHDANG
                     AGLAAPQIGVNLQVVIFGFGHNERYPDAPPVPETVLINPTITPVSLDMEEGWEGCLSV
                     PGLRGAVSRFSMIKYHGFDQYGKPIDRVAEGFHARVVQHECDHLIGKLYPMRINDFAK
                     FGFTEILFPDMDPNSDD"
     gene            complement(4156..6462)
                     /locus_tag="VHV99_16795"
     CDS             complement(4156..6462)
                     /locus_tag="VHV99_16795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008350091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX3637108.1"
                     /translation="MRVKLTAKHPRPASSERAPVRTGSISARKPTRPAGPKPSSSTAG
                     FSGARGEGAGAAGGGGKRPAGAGKPAGAGARAPRAEGSFSRERDAGAARRAPSDRPPR
                     REGAGGAPRRFEGSGERAPRRDDGERAPRRFEGGGDRSERAPRRADGERSQGAPRRFE
                     GTGDRAPRRDDGERAPRRFEGGGERSERAPRRADGERSQGAPRRFEGGGERAPRREDG
                     ARAPRRFEGGTDRSDRAPRRADGERTQGAPRRFEGGGERAPRREDGARAPRRFEGGAD
                     RSDRAPRRDDGERAPRRFEGGGERSQGAPRRSEGSTDRGDRAPRSFEGNRAPRRDDGE
                     RRSFSGPRPGAGRPSEGGPRSERPARGASDRPRFGSDRGAPQERRSGERGPRGDSAPR
                     RFEGERNASGGGERTFAKPVKSGYGDRTDRPARAPRDDRGERAPAAKREFGDRPAHAG
                     ERSERTERGERPARSFDKTMPAAGRRFGDDNRPARADNPRGPASADRAGRDADDAPRT
                     PRRDYEDAPGTLRLSKLMSELGLCSRREADEWIEKGWVLVDGERIDTLGTKVRPDQRI
                     EIDPAAEAAQASQVTVLIHKPVGLVSGQAEDGYQPAITLVTPENRWEGDSSDIRYSVA
                     HLRQLAPAGRLDIDSTGLLVLTQDGRIAKQLIGGHSEIDKEYLVRVSFGEHTTDVESH
                     FPPESLALLCHGLSLDEVPLKPAQVNWQNGEQLRFVLREGKKRQIRRMCELVGLEVIG
                     LKRVRMGQVMLGALPPGQWRYLSADESF"
     gene            complement(6469..9048)
                     /locus_tag="VHV99_16800"
     CDS             complement(6469..9048)
                     /locus_tag="VHV99_16800"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEX3637109.1"
                     /translation="MSSVPVVAQSHATSLKADYKVAKAQLLERFKTASNVDSLMAALA
                     RVTDESLRAVWNTCELPPELALLAVGGYGRGELAPHSDIDILVLLPDAPIAHLEARIE
                     RFISLAWDLGLELGSSVRSVVQCVEEAANDVTVRTSLLEARRITGSAPLFDDFAERYR
                     ETLDPRAFFQAKVLEMRQRHAKFQDTPYSLEPNIKESPGGLRDLQLILWITQAAGFGS
                     SWRELEARGLITEREARELRRNEGFLKTLRARLHVIAGRRQDILVFDLQTQVAESFGY
                     KPTATKRASEQLMRRYYWSAKAVTQLATILIQNIEAQLFPNTSGITRVLSDRFVEKQG
                     MLEIASDDVFQREPNAILEAFLLYEQTPGVKGLSARTLRAIYNARDVMDQHWRRDPEN
                     RRLFMDILKQPAGITHALRLMNQTSVLGRYLLNFRRIVGQMQHDLYHVYTVDQHILMV
                     LRNLRRFAVAEHAHEYPFCSQLIANFDRPWVLYVAALFHDIAKGRGGDHSTLGMADAR
                     RFCRSHGIDGEDGELVVWLVQQHLTMSQVAQKQDTSDPEVIKRFAELVGNERRLTALY
                     LLTVADIRGTSPKVWNTWKAKLLEDLYRNTLAVLGGARPDAHSELKSRQEEALALLRL
                     ETVPEGAQKALWDKLDIGYFLRHDAADIAWQTRVLYRHVETATPIVRARPSPIGEALQ
                     VLVYVKDQPDLFAGICAYFDRNGLSVLDARVSTTRHGYALDNFLVAHTEEDVHYRDIA
                     NLVEQELTARLTGDGTLLPGPSKGRLSRLSRTFPVTPRVDLRADERGQYYILSVSAND
                     RPGLLYSIARVLAEHRVGVASARINTLGERVEDVFLLDGRGLSDNRLQIQVETELLRA
                     IAV"
     gene            complement(9080..9889)
                     /gene="map"
                     /locus_tag="VHV99_16805"
     CDS             complement(9080..9889)
                     /gene="map"
                     /locus_tag="VHV99_16805"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018435517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX3637110.1"
                     /translation="MAITLKNEHDIAQMRVACKLASEVLDYITPFITAGVTTGELDRL
                     CHEYMLQVQGTVPAPLNYQPPGYPPYPKATCISVNDVICHGIPGDKTLKNGDALNIDV
                     TVIKEGYFGDTSRMFIVGEGSIMARRLVQTTFECMWLGIDQVRPGAHLGDIGYAIQKH
                     AEGQGYSVVREYCGHGIGTVFHEDPQILHYGRPGTGFELQTGMIFTIEPMINAGRRDI
                     RTMPDQWTVKTKDRSLSAHWEHTVLVTETGYDVLTVSAGTPARPPIVAATA"
     gene            10381..>10953
                     /gene="rpsB"
                     /locus_tag="VHV99_16810"
     CDS             10381..>10953
                     /gene="rpsB"
                     /locus_tag="VHV99_16810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX3637111.1"
                     /translation="MAVTMRQMLEAGVHFGHQTRFWNPKMAPFIFGHRNKIHIINLEK
                     TLPMYNDALKYARQLAANRGTILFVGTKRQSRDTIAEEAQRAGMPFVNARWLGGMLTN
                     FKTLKVSIKRLKDMEAALEAGETERMSKKEALLFEREMTKLQKSIGGVKDMGGIPDAI
                     FVVDVGYHKIAVTEANKLGVPVIAVVDTNHS"
BASE COUNT         1923 a         3479 c         3578 g         1973 t
ORIGIN      
        1 cgagttgaca ctcggtttga tcggcgcggg tgccgtagtg gtcgggggcg tggtcgtgta
       61 caacgcatgg cagggcgcga aggtgcgccg caagatgccg cgtcccatgc cggccgacac
      121 cgccgaaagc ttcgcgcggg acgatcagga ggaacagagc ccgtttatcg aaccggcccg
      181 gccgaccacg cggcgtgagc cggtcgtggg ttcggacgcg gcaggggaaa ccgcggcggc
      241 gcgcgtcgag ccgacttttg gcgccacggc ggcgccgctc gatacgccgg ccgatattca
      301 ggccgagacg actacgccga acggctatcc ggaagcggaa ggcattgctg aagccgaggg
      361 tggccacgcc gcggagtccg acgaactggc cgagccgatt ctgcccgccg ctacgacgat
      421 ttcgtcggcg ccgccggcca tcgtcgatcg gcgcatcgac tgcatcgtgc cgattcgcct
      481 gaacggcccg gtggccggcg acaaggtgat tccgctcgcg cagcgtctgc gccgcgccgg
      541 cagcaagccg gtgcatatcg aaggcaagct cgaaggcggc gcgtgggaat tgctgcaaaa
      601 cggcgcacgc tatgaagagt tgcgcgcggc cgcgcaattg gccaatcgga gtggggcgct
      661 caacgagctg gagttctcgg agttcgtgac cggcgtgcag cagtttgccg atgcgctcga
      721 cgcatcgccg gaatttcccg acatgctcga aacggtcgcg atggcgtgcg agctcgacgg
      781 cttcgccgcg cagtgcgatg cgcaactgtc gatcaacgtg ctttccgacg gcgcgccgtg
      841 gtcggccaac tacgtccagg cagtcgcgtc gcaggacggc ttgctgctct cgcgcgacgg
      901 tacgcgcttc gtcaaactcg acgcgaagca gagcccggtg ttcatgctgc aattcggcga
      961 caccaatttc ctgcgcgacg acctgaccta caagggcggc cagatgatta cgctggtgct
     1021 cgacgtgccg gtcgcggacg aagacatcct gccgttccgc ctgatgtgcg actacgcgaa
     1081 atcgttggct gagcgtatcg gtggacgggt ggtcgacgac gggcgccgtc cgctgccgga
     1141 aaccgcgctg ctggcgatcg aaaagcagct gatgacgctc tacgcgcggc ttgaacaggc
     1201 gggaatcccg gcgggttcgc cagctacccg gcgtttgttc agccagtaac ggcctggctg
     1261 tttcatgcct gacccaacgg ccgcgcgatg tgcggccgtt gacgcttctg gcgcaaggac
     1321 gacgcttgaa actggcctat tcggccgatg taaaggggca cgctttcctg cgataatcct
     1381 ctgtctgaat ttcactgaag aagcttccga cagcatggcc cgaactcccg cctcaaacct
     1441 tgcaaccagc gctccggcag agcgggccgc gtggctgcgc gcggaactcg aacgcgccaa
     1501 ttacgcgtat tacgtgctcg accagccgga cctgccggat gccgaatacg acacgctctt
     1561 caaggaactc gagcgcatcg agacggagca tccggacctg atcgtggccg attcgcccac
     1621 ccagcgcgtg ggcggcgagg cgtcgagcgg tttcgagccg gtcgtgcacg atcaacccat
     1681 gctctcgctg aacaatggct tcgcggatga agacatcgtc gcgttcgaca agcgcgtcgc
     1741 cgacgcgctc ggcaagaacg cgagcgaacc gcccgcaccg gtcgattacg cggctgaact
     1801 gaagttcgac ggccttgcca tctcgctgcg ttacgtcgac ggcatgttcg tccaggcgtc
     1861 cacgcgcggc gacggcgcca ccggcgagaa cgtcaccgag aacgttcgca cgatccgttc
     1921 cattccactc aagctcaagg gcaagcgcgt gccgcgggtt cttgacgtgc gcggcgaagt
     1981 gctcatgttc aagcgcgact ttgaacgcct gaacgagcgc cagcgggccg ctgagcagcg
     2041 ggaattcgcc aatccgcgca acgccgcggc aggcagtctg cggcagctcg attcgaagat
     2101 caccgcgcaa cggccgctgt cgtttttcgc ttatggaatc ggcgtgctcg aaggcattgg
     2161 gatgccggcc acgcatagcg agctgctcga ctggtacaag gaactcggct tgccggtgaa
     2221 tggcgaacgg gcggttgtgc aaggcgccga aggcttgctg gggtttttcc acaaagtagg
     2281 cgagaagcgc gacaagctgc cgtacgacat cgacggcgtc gtctacaagg ttaaccggcg
     2341 cgacgagcag gatgcgctcg gctttgtctc acgcgcgccg cgtttcgcct tggcgcacaa
     2401 atttcccgcg caggaagcgc tgaccagact cgtcgcgatc gacgtgcagg tcggccgcac
     2461 cggcgcgatc acgccggttg cgcggttgga gccggtgttc gtcggtggcg cgacggtgac
     2521 gaacgcgacg ttgcacaatg aagacgaagt gcgccgcaag gatatccgca tcggcgacac
     2581 ggtgatcgtg cggcgcgccg gcgacgtgat tcccgaagtg gtgagcgcgc tgctcgagcg
     2641 ccgcccggaa gacgcccgcg agttcgtcat gcccacgcag tgcccggtgt gcggctcgag
     2701 catcgaacgt ctgccggacg aggcgattgc gcgctgtacg ggtggcctgt tttgtccggc
     2761 gcagcgcaag caggcgctgt ggcatttcgc gcagcggcgc gcgctggata tcgacgggct
     2821 cggcgaaaag atcatcgatc aactcgtcga gcagaatctg gtgcgtacgc cggccgatct
     2881 gttcaacgtc ggcttcgcga cgcttgctga actcgaccgc ttcgccgaaa agtccgcgca
     2941 gaatctgctg gactcgctcg aaaaagccaa acacaccacg ctcgcgcgct tcatttacgc
     3001 gctcggtatc cggcatgtgg gcgaatcgac ggcaaaagat ctggccaaac atttcggctc
     3061 gctgaatccg atcatggacg cctcggtcga agaattgctg gaggtgaacg atgtgggtcc
     3121 tgtcgtggcc gagtcgattc atcagttttt tgccgaggag cacaaccgca cggtgatcga
     3181 gcaactccgc gcgccgggca aggtcacctg gccggaagga ccacccgcgc caaaggcgcc
     3241 gcaaggcgtg ctggccggca agacggtcgt attgaccggc accttgccga ccttcgcccg
     3301 cgaagaggcg aaagaaatgc tcgaagcggc cggagcgaag gtcgccggtt ctgtgtcgaa
     3361 gaaaacggac tacgtcgtgg cgggggccga agctggcagc aagttggcga aagccgagga
     3421 actcggcatc cccgtactcg acgaagatgg tatgcgtaag cttctggagg ggcagtcatg
     3481 atccgcgaaa ttctcaagat gggcgatccg cgccttttgc aaattgccga tccggtcgat
     3541 cacttcgaca cacccgaact gcatgaactc gtcaaggaca tgttcgagac catgcacgac
     3601 gcgaacggcg cggggctcgc cgcgccgcag atcggcgtca atctgcaggt ggtgatcttc
     3661 ggcttcgggc acaacgaacg ctatccggat gcgccgccgg tgccggagac cgtgttgatc
     3721 aatccgacca tcacgccggt gtcgctggat atggaagagg gctgggaagg ctgcctgtcc
     3781 gtgccgggct tgcgcggcgc ggtaagccgg ttttcgatga tcaagtatca cgggttcgat
     3841 caatacggca agccgatcga ccgcgtcgcc gagggctttc acgcgcgtgt cgtgcagcat
     3901 gagtgcgacc acctgatcgg caagctgtac ccgatgcgga tcaacgattt cgcgaagttc
     3961 ggcttcacgg aaatcctgtt tccggatatg gacccgaata gcgacgactg aggccgtatc
     4021 ccgggctgcg gcgcatgcat tgtcgtgtgc gccgccggca taaaaaaacc cacgttttca
     4081 catgggtttc tggttggtcg agtgtcacgg tgcggcaccg tgagttttcg ctgtttgagc
     4141 gtcaagctcc cggcatcaaa acgactcgtc cgccgacaga tagcgccatt ggcccggcgg
     4201 caatgcgccc agcatcacct ggcccatccg cacgcgcttc aggccaatca cttcgaggcc
     4261 gaccagttcg cacatccggc ggatctggcg cttcttgcct tcgcgcagca cgaaacgcag
     4321 ttgctcgccg ttttgccaat tgacttgggc gggtttcagc ggcacctcgt cgagcgagag
     4381 gccgtggcac aagagcgcga ggctttccgg cgggaagtgg ctttcgacgt cagtggtgtg
     4441 ctcgccgaac gacacgcgca ccagatactc cttgtcgatc tccgaatggc cgccgatcag
     4501 ctgcttggcg atgcgaccgt cctgcgtcag caccagcaag cctgtcgaat cgatgtcgag
     4561 acgcccggcc ggcgcgagtt ggcgcagatg cgcgaccgag tagcggatgt ccgaactgtc
     4621 gccttcccag cgattttccg gcgtgaccag cgtgatcgcc ggctgatagc cgtcttcggc
     4681 ctggcccgat acgaggccca cgggcttgtg gatcaggacc gtcacctggc tcgcttgcgc
     4741 ggcctcggca gccgggtcga tctcgatgcg ctggtccggg cgaaccttgg tgccgagcgt
     4801 gtcgatccgc tcgccgtcga ccagtaccca gcctttttcg atccactcgt ccgcttcgcg
     4861 gcgtgagcac aggccgagtt ccgacatcag cttggacagg cgcagggtgc ccggtgcgtc
     4921 ttcgtaatcg cggcgtggcg tgcgcggggc gtcatcggcg tcgcgtccgg ctctgtctgc
     4981 cgatgctggg ccacgcgggt tgtcggcacg tgcggggcga ttgtcatcgc caaagcggcg
     5041 gccggcagcc ggcattgtct tgtcgaaact gcgagccggg cgttcgcccc gctcggtacg
     5101 ttcgctccgt tcgccggcat gcgcgggacg gtcgccgaat tcgcgtttgg cggccggtgc
     5161 ccgttcgccg cggtcgtcgc gcggtgcgcg cgccggacgg tccgtgcgat cgccatagcc
     5221 gcttttgacg ggcttcgcga aagtgcgctc gccaccgccg gacgcgttgc gctcgccttc
     5281 gaaacggcgc ggtgcgctgt cgccgcgcgg cccgcgctcg ccgctacggc gttcttgcgg
     5341 ggcgccgcgg tcactgccga agcgcggccg atccgaggcg ccgcgcgccg gacgctcgct
     5401 gcgcggtcca ccttccgagg gacggccggc acccgggcgc gggccgctga acgagcggcg
     5461 ttcgccgtcg tcacggcggg gggcgcggtt gccttcgaaa ctgcgcgggg cgcggtcacc
     5521 gcgatccgtg cttccttcgg atcggcgggg ggcgccctgg ctgcgctcgc cgccgccttc
     5581 aaatctacga ggcgcacgtt caccatcgtc ccgacgcgga gcgcggtcgc tgcggtcggc
     5641 gccaccttca aaccggcgcg gcgcccgagc gccatcttcc cgacgcggag cgcgttcgcc
     5701 gccgccctca aaccggcgcg gtgcgccttg agtgcgttca ccatcagcac ggcgcggagc
     5761 gcggtcgcta cggtcggtgc caccttcaaa ccggcgcggc gcccgagcgc catcttcccg
     5821 acgcggagcg cgttcgccgc cgccctcaaa ccggcgcggc gcaccttgac tgcgctcacc
     5881 atcagcgcga cgcggagcac gttcgctacg ttcgccgcca ccttcaaacc tacggggcgc
     5941 acgctcacca tcgtcccgac gcggagcgcg atcaccagta ccttcaaacc ggcgcggcgc
     6001 accttggctg cgctcaccat cagcgcgacg cggagcacgt tcgctacgat cgccaccacc
     6061 ttcaaaccta cggggcgcac gctcaccatc gtcccgacgc ggtgcccgct cgccgctccc
     6121 ctcaaaccgg cgcggcgcac cgccggcccc ttcacgccga ggcggccgat ccgacggcgc
     6181 ccggcgcgca gcgccagcat cccgttcacg ggaaaatgat ccttcagccc gtggcgcacg
     6241 cgcaccggcg cccgccggct tacctgctcc agcgggccgc ttgccgccgc cacccgctgc
     6301 gccagcgcct tcaccccgcg ccccgctaaa acccgccgtc gacgaggacg gtttcggccc
     6361 ggccgggcgt gtcggcttac gcgccgagat gcttccggta cggacagggg cgcgttcgga
     6421 cgaagccggc cgcgggtgct tggctgttaa tttgactcgc atgaaatctc acactgcaat
     6481 cgcgcgcagc agttcggtct cgacctgaat ttgcaggcgg ttatccgaca gaccgcgtcc
     6541 atccagcagg aacacgtctt cgacgcgttc gccgagcgta ttgatccgcg ccgacgcaac
     6601 gccgacccgg tgctcggcca gcacgcgcgc gatcgaataa agaaggcctg gccggtcgtt
     6661 cgcagacacg gacaggatgt aatattggcc gcgctcgtcg gcccggagat cgacgcgcgg
     6721 cgtgacaggg aaggtccgcg acagccgtga caaacggcct ttcgacggtc ccgggagcag
     6781 ggtgccgtca cccgtgaggc gtgcggtcag ttcctgttcg accagattgg caatatcgcg
     6841 ataatgcacg tcttcttcgg tatgcgcgac gaggaaatta tcgagcgcgt acccgtgacg
     6901 ggtcgtgctg acccgcgcat cgagcaccga caagccgttg cggtcgaaat acgcacaaat
     6961 gccggcgaac agatcgggct gatccttgac atacaccagc acctgaagcg cttcgccgat
     7021 cggcgacgga cgggctcgca cgatcggcgt cgcggtttcg acatggcggt acaacacgcg
     7081 ggtttgccag gcgatatccg ccgcgtcgtg acgcaggaaa tagccgatgt ccagtttgtc
     7141 ccacagcgcc ttttgcgcgc cttccggcac ggtttcgagg cgcagcagcg ccagcgcctc
     7201 ttcctggcgc gatttcagtt ccgaatgcgc gtcaggccgt gcgccgccga gtaccgccag
     7261 cgtgtttcgg taaaggtcct cgagcagctt ggctttccag gtgttccaga ctttcgggct
     7321 ggtgccgcga atgtcggcga cagtcagcag gtagagcgcg gtcagccggc gctcgttgcc
     7381 gaccagttcg gcaaaccgct tgatgacttc agggtcgctc gtgtcctgct tctgcgcgac
     7441 ctggctcatg gtcagatgct gctggaccag ccagacgacc agctcgccgt cttcgccgtc
     7501 aatgccatga ctgcggcaga agcgccgcgc gtcggccatg ccaagcgtgg aatggtcgcc
     7561 gccgcggccc ttggcgatgt cgtgaaacag cgccgccacg tacaacaccc acggccggtc
     7621 gaaattggcg atcaactgac tgcagaacgg atattcgtgc gcgtgctcgg cgaccgcgaa
     7681 gcggcgcaga ttgcgcaaca ccatgaggat gtgctgatcg acggtgtaga cgtgatacag
     7741 gtcgtgctgc atctgtccga caatccgccg gaagttcagc agatagcgcc cgagcacgct
     7801 agtctggttc atcagccgca gcgcgtgcgt gatgccggcc ggctgcttca ggatgtccat
     7861 gaacaggcgc cggttttccg gatcgcgccg ccaatgctga tccatcacgt cgcgggcgtt
     7921 gtagatcgcg cgcagcgtgc gcgccgacaa acccttcacg cccggcgtct gttcgtagag
     7981 caggaaagct tcgaggatcg cgttcggctc gcgctggaat acgtcgtcgc tggcgatttc
     8041 cagcatgcct tgcttctcga cgaaacggtc cgacagcacg cgcgtgatgc cgctcgtgtt
     8101 ggggaagagt tgcgcctcga tgttctggat caggatcgtc gcgagttgcg tgacggcctt
     8161 ggcggaccag taatagcgac gcatcagctg ctcgctggca cgcttggtgg ccgtcggctt
     8221 gtagccgaag ctttcggcta cctgcgtttg caggtcgaac accagaatgt cctgccggcg
     8281 gccggcgatc acatgcagcc gggcacgcag cgttttcagg aagccttcgt tgcggcgcag
     8341 ctcgcgcgct tcacgctcgg tgatcaggcc gcgtgcttcg agttcgcgcc agctgctgcc
     8401 gaaaccggcc gcctgggtaa tccacagaat cagttgcaga tcgcgcaggc cgcccgggct
     8461 ttccttgatg ttcggttcga gcgaatacgg cgtgtcctga aacttggcgt gccgctgacg
     8521 catttcaagc actttggcct ggaagaatgc gcgcgggtcg agcgtttcgc ggtagcgctc
     8581 cgcaaagtcg tcaaagagcg gggcgctgcc cgtaatgcgc cgcgcctcga gcagcgaggt
     8641 gcgcacggtg acgtcgttgg cggcttcttc gacgcactgc acgacgctac gcacgctgct
     8701 gccaagttcg agccccagat cccacgccag gctgataaag cgttcgatgc gcgcttcgag
     8761 atgggcaatc ggcgcgtcgg gcagcagcac cagaatgtcg atgtcggaat gcggcgcgag
     8821 ttcgccgcgc ccgtaaccgc ctaccgccag caaggccagc tccggcggca gctcacaggt
     8881 gttccagacg gcgcgcagcg actcgtccgt gacgcgcgcg agcgctgcca tcagcgagtc
     8941 gacgttggag gcggtcttga agcgttccag caactgggct ttggccactt tatagtccgc
     9001 cttgagcgac gtggcatggg attgggcgac gactggaaca ctactcattg gcaggcgggc
     9061 ggataaggtc agaagtcgat caggcggtgg ccgcgacgat cggcggccgc gcgggcgtac
     9121 cggccgagac agtcagcacg tcgtagccgg tttcggtgac gagcacggtg tgctcccagt
     9181 gcgccgacag gctgcggtct ttggtcttga cggtccactg atccggcatg gtgcggatgt
     9241 cgcgccgacc ggcgttgatc atcggctcga tcgtgaagat catgccggtc tgcaactcga
     9301 acccggtgcc gggacggccg tagtgcagga tctgcggatc ttcgtggaac acggtgccga
     9361 tgccgtggcc gcaatattcg cgcaccacgc tgtagccttg cccttcggca tgcttctgga
     9421 tggcgtagcc gatatcgccg agatgggcgc ccgggcgcac ctgatcgatg ccgagccaca
     9481 tgcattcgaa ggtggtctgg accagccgcc tggccatgat cgagccttcg ccgacgataa
     9541 acatccggct ggtgtcgccg aaataacctt ccttgatcac cgtgacgtcg atattcagcg
     9601 cgtcgccgtt cttcagcgtc ttgtcgcccg gaatgccgtg gcagatcacg tcgtttaccg
     9661 aaatgcaggt ggctttcggg taaggcggat agccgggcgg ctgatagttc agcggcgccg
     9721 gaaccgtgcc ttgtacctgg agcatatatt cgtgacacag ccggtcgagt tcgccggtgg
     9781 tgacgccggc cgtgatgaac ggcgtaatgt agtcgagcac ttcgctagca agcttgcagg
     9841 cgacgcgcat ttgcgcgata tcgtgttcgt ttttgagcgt aatagccatg agtcgggcct
     9901 gaaatgcgat ttattgcggg attatcgcac catattcagg ccgccgcagg cttttgcaat
     9961 agggctggag ccttttgcgg cggtgcctga gtcgtctttt gcggccatcc gcacagcttc
    10021 ggcggccgcc ttcgcccggg ccgcggcagg tgcccgggtg caacccggct acgtcaaatg
    10081 ggagctaccg caggttgagt cggccgctgg tcgcgtgcta taatctttgg ctaagtcgat
    10141 ctctgtctta attttgttgc ttgattgctg ttctaacgct gtttctagcg ggtacgcaca
    10201 agggcgcaaa ctgacggaaa aggcttctcg tagcgatagt tgcctgcgct acgggctaag
    10261 gcggtatcaa ggcagcagac tcgcaagccg gcgcacccag ggtgtccgtc gcgtttcgcc
    10321 ggaaaggcag cgcggcagat acggcagccg gcttaagacc caaccctcgc ggagattttc
    10381 atggcagtta ccatgcgtca aatgctggaa gccggtgtcc acttcggtca ccaaacgcgc
    10441 ttctggaacc cgaagatggc ccccttcatt ttcggtcatc gcaacaagat tcacatcatc
    10501 aacctcgaaa agacgctgcc gatgtacaac gacgcgctga agtacgcgcg tcaactggca
    10561 gcgaatcgcg gcacgatcct gttcgtcggc acgaagcgtc aatcgcgcga cacgatcgct
    10621 gaagaagcgc agcgcgcagg tatgccgttc gtcaacgcac gctggctcgg cggcatgctg
    10681 accaacttca agacgctgaa agtttcgatc aagcgcctga aggacatgga agcggcgctg
    10741 gaagcaggcg aaaccgaacg catgagcaag aaggaagcgc tgctgttcga acgcgaaatg
    10801 accaagctgc aaaagtcgat cggcggcgtg aaggacatgg gcggtattcc ggacgcgatc
    10861 ttcgtggtcg acgtcggcta ccacaagatt gccgttaccg aagctaacaa gctcggcgtg
    10921 ccggttatcg ccgtggtcga cacgaaccac tcg
//
LOCUS       DATASH010000617         4849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6034381,
            whole genome shotgun sequence.
ACCESSION   DATASH010000617 DATASH010000000
VERSION     DATASH010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4849
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6034381"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="VHV99_16815"
     CDS             <1..723
                     /locus_tag="VHV99_16815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX3637112.1"
                     /translation="ALTQGVFMHPLPMIAQIALTLLIVIPMGPYVYRLAYEPIAEATT
                     LLLLIVAVAVHFAMVGLGLVMFGAEGSRTTAFSDATFNIGTLTISGQSLWVVGTAVVL
                     IGALYLYFDRSISGKALRATSVNRLGARLVGIGTTQAGRLAFTLAAGLGALCGILVAP
                     LTTIYYDSGFLIGLKGFVGAIIGGLVSYPLAAAGSILVGLLESYSSFWASAYKEVIVF
                     TLIIPVLLWRSLASPHSEEEEE"
     gene            728..2512
                     /locus_tag="VHV99_16820"
     CDS             728..2512
                     /locus_tag="VHV99_16820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011655609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HEX3637113.1"
                     /translation="MKRIMQNKFFWLFLVVLFALPVLPHPIHVPEYWVTLLNYIGLYS
                     IVAIGLVLLTGIGGMTSFGQAAFVGIGAYATAYLTTTYGVSPWLALIAGVVVTALIAL
                     MLGLVTMRLSGHFLPLGTIAWGLALFYLFGNMEMLGKYDGINGIPVLNVFGINLESGR
                     HIYYLIWVVVLGAVISVQNLLNSRPGRAIRALRGGGMMAEAMGVNTAWMRVVIFVYAA
                     VLASISGFLYAHLQRAVNPTPFGLNHGIEFLFMAVVGGVSHVWGAVLGAAILTVLQDY
                     LQTLLPKLLGENGNFEVIVFGILMVLLLQYARQGVWPFVARFFPKGPRAHLSDHAEPL
                     PQRSKPTAGESLLTVNKARKQFGGLVAVNDVSFDVKAGQIIGLIGPNGAGKSTTFNLV
                     TGVLQATSGEITFRGERIDSLSSREIVKRGIGRTFQHVKLLPGMTVLENVAIGAHLRG
                     HAGVWRSVARLNGAEEARLMAEAARQIRRVGLEQHMYDEAGSLALGQQRILEIARALC
                     CDPTLLLLDEPAAGLRYQEKMQLADLLRRLKAEGMSVLLVEHDMDFVMNLTDRLVVME
                     FGTRIAEGLPQDVQQDPAVLEAYLGGVE"
     gene            2524..3276
                     /locus_tag="VHV99_16825"
     CDS             2524..3276
                     /locus_tag="VHV99_16825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3637114.1"
                     /translation="MNAAVPILDVNGLSVRYGKVEALHGAAIKVRPGQIVSVIGPNGA
                     GKSTLLNAIMGALPTTGHAKGSVLYQGEDVSAVPVEKRVARGMCLVPEKRELFASMTV
                     EDNLVLGAYRRKRAGERNFLDQLEPVFTLFPRLKERRKQAAGTLSGGERQMLAVGRAL
                     MGKPDLLMLDEPSLGLAPLIVKEIFHIISALRQTGVATLLIEQNARAALQISDYGYVL
                     ETGELALEGPAADLAQNPRVIETYLGLAKKVA"
     gene            3452..>4849
                     /gene="mnmG"
                     /locus_tag="VHV99_16830"
     CDS             3452..>4849
                     /gene="mnmG"
                     /locus_tag="VHV99_16830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012402386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEX3637115.1"
                     /translation="MLFPTEFDVIVVGGGHAGTEAALASARMGNTTLLLTHNIETLGQ
                     MSCNPSIGGIGKGHLVKEVDALGGAMAAATDEGGIQFRILNSSKGPAVRATRAQADRL
                     LYKQAIRHRLENQPNLWLFQQAVDDLMVEGDRVVGAVTQVGIRFRARAVVLTAGTFLD
                     GKIHVGLNNYTGGRAGDPAAVSLSARLKELKLPQGRLKTGTPPRIDGRTIDFSKLEEQ
                     PGDLDPVPVFSFLGRVEQHPRQVPCWVTHTNERTHDIIRGGLDRSPMYTGVIEGVGPR
                     YCPSIEDKIHRFASKESHQIFLEPEGLTTNEFYPNGISTSLPFDVQLELVRSMHGLEH
                     AHILRPGYAIEYDYFDPRGLKASLETKVISGLFFAGQINGTTGYEEAAAQGLLAGINA
                     GLHVQGKDAWCPRRDQAYLGVLVDDLVTRGVSEPYRMFTSRAEYRLSLREDNADIRLT
                     EVGRELGVVDDIRWDA"
BASE COUNT          844 a         1321 c         1620 g         1064 t
ORIGIN      
        1 gcgcttacgc aaggcgtttt catgcatccg ctgccgatga tcgcgcagat cgcgttgacg
       61 ctgctgatcg tgattccgat ggggccgtat gtttaccggc tcgcgtacga gccgatcgcg
      121 gaagcgacca cactgctgct gttgatcgtg gcggtggcgg tgcacttcgc gatggtgggc
      181 cttggtctcg tgatgttcgg tgcggaaggt tcgcgcacca ctgcgttttc cgatgcgacc
      241 ttcaacatcg gcacgctgac gatttccggg caaagcctgt gggtcgtcgg cactgcggtt
      301 gtgttgatcg gcgcactgta tctgtacttc gatcgctcga tctcgggcaa ggccttgcgc
      361 gcgacgtcgg tgaaccggct cggcgcgcgg ctcgtcggta tcggcacgac gcaagcgggg
      421 cgtctcgcat tcacgttggc ggccggcctc ggcgcgctgt gcggcatcct cgtcgcgccc
      481 ttgaccacca tctactacga ctcgggcttc ctgattggtt tgaagggctt cgtgggggcg
      541 attatcggcg gcctcgtcag ctatccgctg gcggccgcgg gctcgatcct ggtcggcctg
      601 ctggaatcgt attcgtcgtt ctgggcgagc gcttataagg aagtgatcgt gttcacgctg
      661 atcattccgg tgttgctgtg gcggagtctg gccagcccgc actccgaaga ggaagaggag
      721 tgacacgatg aaacggatca tgcaaaacaa gttcttctgg ctgttcctcg tggtgctgtt
      781 cgcgttgccg gtgttgccgc atccgattca tgtgcccgag tactgggtga cgctgctcaa
      841 ttacatcggc ttgtattcga ttgttgcgat cggtctcgtg ctgctgaccg gtattggtgg
      901 gatgacgagc ttcggacagg cggcgttcgt cggcatcggc gcgtatgcga ccgcgtatct
      961 gacaacgacg tatggcgtgt cgccgtggct cgccctgatc gcgggcgtag tggtgacggc
     1021 gttgatcgcg ctgatgctgg gtctcgtgac gatgcggcta tcgggccact ttttgccgct
     1081 cggcacgatc gcgtgggggc tcgccctgtt ctatctgttc ggcaacatgg agatgctcgg
     1141 caagtacgac ggcattaacg ggattccggt gctgaatgtt tttggcatca acctggagtc
     1201 cggccgccat atctattacc tgatctgggt ggtggtgctc ggcgcggtga tctctgttca
     1261 aaatctgctt aatagcaggc cggggcgggc gatccgcgca ttgcgcggcg gcggcatgat
     1321 ggctgaagcg atgggcgtca acacggcgtg gatgcgcgtg gtgatcttcg tctatgccgc
     1381 agtgctggcg tcgatctccg gtttcttgta cgcgcacctg cagcgcgcgg tgaatccgac
     1441 gccctttggt ctgaaccacg gcatcgagtt tctgttcatg gcggtggtgg gcggcgtgtc
     1501 gcatgtctgg ggcgctgtgc tcggtgcagc gatcctgacc gtgctgcagg actatctgca
     1561 aacgctgctg cccaagctac tcggcgagaa cggtaacttc gaagtgatcg tgttcggcat
     1621 cctgatggtg ttgctgctgc aatacgctcg tcaaggcgtc tggccgtttg tcgcacggtt
     1681 tttccccaag gggccccgcg cgcacctgtc ggatcatgcc gagccgttgc cgcagcgcag
     1741 caagccgact gcgggggaaa gcctgttgac cgtgaataaa gcgcgcaagc agttcggcgg
     1801 cctggtcgcg gtgaacgatg tcagcttcga cgtgaaagca gggcagatca tcggcttgat
     1861 tggtccgaat ggcgccggca aatcgaccac cttcaatctg gtcacgggcg tgttgcaagc
     1921 gacgagcggc gaaatcactt ttcgcggcga gcgtatcgat tcgctgagtt cacgtgaaat
     1981 cgtcaagcgg ggtattggcc gcaccttcca gcacgtcaag ctgctgcctg ggatgacggt
     2041 gctggagaac gttgcgatcg gcgcgcattt gcgtggccac gccggtgtgt ggcgcagcgt
     2101 cgcgcggctg aacggcgctg aagaagcgcg cctgatggcc gaagcggcgc gccagattcg
     2161 ccgcgtcggc ctcgagcaac atatgtacga cgaggcgggt agccttgcgt tggggcaaca
     2221 acggatcctc gaaattgctc gggcgctctg ctgtgacccg accttgttgc tgctggacga
     2281 acctgccgcc ggtctgcggt atcaggaaaa aatgcagttg gcggacttgc tgcgccgcct
     2341 caaagctgaa ggcatgagcg tgctgttggt cgaacacgat atggatttcg tgatgaatct
     2401 caccgatcga cttgtggtga tggagttcgg cacgcggatc gcggaaggcc tgccgcagga
     2461 cgtccagcaa gatccggctg tgcttgaggc ttatctgggc ggggtggagt gatggaaaac
     2521 acgatgaatg cggctgtacc gattcttgac gtgaacgggt tgtccgtccg ctatggcaag
     2581 gtggaggcgc tgcatggcgc ggcaatcaag gtgcggcccg ggcagatcgt ctcagtgatt
     2641 ggtccgaatg gcgcgggcaa gtccacgttg ctgaacgcga tcatgggcgc attgccgact
     2701 acgggccatg cgaagggctc ggttctctat caaggtgaag acgtcagcgc ggtaccggtt
     2761 gaaaagcgcg tcgcgcgcgg catgtgtctt gtaccggaaa agcgcgagct gttcgcgtcg
     2821 atgacggttg aagataatct ggtcctcggc gcgtatcggc gcaagcgggc aggggagcgc
     2881 aactttctcg atcaactcga gccggtcttt acgctgtttc ctcgcttgaa agagcgccgg
     2941 aagcaggcag ccgggacgtt gtccggcggt gagcggcaga tgctcgcggt cggccgtgcg
     3001 ttgatgggca aacccgatct gctcatgctc gacgaaccga gtctgggcct cgcaccgctg
     3061 atcgtgaagg aaattttcca cattattagt gcgctgcggc agaccggcgt cgcgacactg
     3121 ctaatcgagc agaatgcacg cgctgcgctg cagatctccg actacggtta cgtgcttgaa
     3181 accggtgaat tggcgctcga agggcctgcg gctgatctgg cgcagaatcc gcgtgtgatc
     3241 gaaacctatc tggggctggc caagaaagtg gcttaatttc tcattttcgt tgagttcgct
     3301 gcggttcaaa gcggtgtgtt tcacgtgaaa cacaccgctt ttttcttgtc tggccattcg
     3361 gacgaaattc gggcctaaac cgaacccgtt ataattcgcc catacatttt cctttgaaag
     3421 gctcacgcga cgatctggcg tgagatccgc gatgcttttt cccacagaat ttgacgtaat
     3481 cgtcgtcggc ggcggtcatg ccggcactga agccgctttg gcttcggcac gcatgggtaa
     3541 taccaccctg ctgctgacgc acaacatcga aaccctcggt cagatgagtt gcaatccgtc
     3601 catcggcggt attggcaagg gccatctggt gaaggaagtc gatgcgcttg gcggtgcgat
     3661 ggcggccgcg acggatgagg gcgggatcca gttccgcatc ctcaattcgt cgaaggggcc
     3721 tgctgtccgt gcgacccgcg cgcaggcgga ccgtctgctg tacaagcagg caatccggca
     3781 tcggctggag aaccagccga acctctggct gttccagcag gcggtcgacg atttgatggt
     3841 ggaaggcgac cgggtggtcg gtgctgtgac gcaggtgggg attcgtttcc gcgcgcgcgc
     3901 ggttgtgctg acggcgggga cgttcctcga cggcaagatt cacgtgggtt tgaacaacta
     3961 caccggtggc cgtgccggtg atccggcagc ggtgtcgcta tcggcgcgcc tcaaggaact
     4021 gaagctgcca cagggtcgcc tcaagactgg gacgccgccg cgtatcgacg gtcggacgat
     4081 cgacttttcg aagcttgaag agcagccggg cgatctggat ccggtgccgg ttttctcgtt
     4141 tctggggcgc gtcgagcagc atccgcgcca agtgccgtgc tgggtcacgc atacgaatga
     4201 gcgcacgcac gacatcatcc gcggcggtct cgaccgctcg ccgatgtaca cgggcgtgat
     4261 tgaaggggta gggccgcggt attgcccatc gattgaggac aagattcacc gctttgcgtc
     4321 gaaagaatcg catcagattt tccttgaacc ggaagggctg acgacgaacg agttctaccc
     4381 caatgggatt tccaccagcc tgccgttcga cgtgcagttg gagctcgtgc gctcgatgca
     4441 cggcttggag catgcccaca tcctgcggcc gggctatgcg atcgaatacg actacttcga
     4501 tccgcgtggg ctgaaggcat cgctggaaac gaaggtgatc agcggtctgt tcttcgcggg
     4561 ccagatcaat ggcacgactg gctatgaaga agccgctgca caaggtttgc tggcggggat
     4621 caatgcgggc ttgcacgtgc agggtaagga tgcatggtgc ccgcgccgcg atcaggccta
     4681 cctgggtgtt ctagtcgacg atctggtgac gcgcggggtg tccgagccgt atcggatgtt
     4741 cacgagccgc gccgaatatc gtctgagctt gcgtgaagat aacgccgata tacgtctgac
     4801 ggaggttggt cgtgagcttg gtgtggtcga cgacatccgg tgggatgca
//
LOCUS       DATASH010000618         1862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1684499,
            whole genome shotgun sequence.
ACCESSION   DATASH010000618 DATASH010000000
VERSION     DATASH010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1684499"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..406)
                     /locus_tag="VHV99_16835"
     CDS             complement(128..406)
                     /locus_tag="VHV99_16835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637116.1"
                     /translation="MNTLMIKDLSITEQLDSKAMSAVRGGFGYPSSSYYNFSPVVAPN
                     NSKHVNATQLISNEVNIQNANGNNAAFVAGITTTIDPHVSSSNNINVY"
     gene            822..>1862
                     /locus_tag="VHV99_16840"
     CDS             822..>1862
                     /locus_tag="VHV99_16840"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX3637117.1"
                     /translation="MASLAHVIRDYQNGELSRDEFVAQLDSTLTTEGLGPTQLLEMLG
                     VAHRKAPLPDDLYAEVRRRIEQLRVSNVAAGGEETGMQTAVEIPSVRSASSAGTASTA
                     GAANQDQIKGTGDTLNNRFVLEECLGVGGMGTVYKALDLRKLEASDRKPYVAIKVLNV
                     QFRGNPNSLVALQREARKAQVLAHRNIIAVYDFDRDGPIVYLTMEYLTGKPLSQLLRT
                     PGYKGMPVRAALPIVRGMCNALAYAHEHGFVHCDFKPANVFLTTNAEVKVIDFGIARV
                     FQRPEEESDATVFDPGTLGALTPAYASPEMIEHREPDPRDDIYALGCITYELLTGHHP
                     FDRLSATQARNSD"
BASE COUNT          342 a          544 c          602 g          374 t
ORIGIN      
        1 gtcggcgcgg ggatcgggcg gtgacgctgg ggtcgtgtgg aaagaacgat tggatgcgaa
       61 atctttttga cgcgcgagtc ttgcgcgtcc cgcggagaca tgagcgcctc cgcggcggct
      121 acgccgttca gtacacgttg atgttgttgc tcgagctcac gtgcgggtcg atcgtcgtgg
      181 tgatgccggc gacgaacgct gcgttgttgc cgttggcgtt ctgaatgttc acttcgttgc
      241 tgatcaactg cgtggcgttg acgtgcttgc tgttgttcgg cgcgactacc gggctgaagt
      301 tgtagtacga cgacgaggga tagccgaagc cgccgcgcac ggcgctcatg gccttgctgt
      361 cgagttgttc ggtgatggac aggtctttga tcatcagcgt gttcatggta aatctcctgg
      421 aagggaacgt cacagtcttg cggttgggtt cgagcatctt gtctgctcga gtgtcactaa
      481 cgcatgggct gtgccagttt tattcgtggc tcctaagaaa gttgccgaac gcttgatgca
      541 caagggtttc gcgacgcgcc gccgcgttgg ggccgccgca cggtcgtcta cagacgctca
      601 gagcctgacg gcgcgtaccc aacaacgcgc gggattctgt tggatgccgc aagacattcg
      661 cggtgcatgc acgcagcggg ccaatcctga tttgcgaaag cgtgggccgg gagctagtat
      721 cgaagcggca catgtaactg attacgacag gttttttatc agcccgaggg ttgtcggttg
      781 catgcgatgc ggcccaacgg cagcggcggc gcttcgcaca aatggctagc cttgcgcatg
      841 tgattcgcga ctatcagaac ggcgagctgt cacgtgacga gttcgtcgct caactcgaca
      901 gcacgttgac gaccgaaggc ctcggtccaa cgcagctgct ggaaatgctc ggcgttgcgc
      961 atcgcaaagc ccctctgcct gacgatctct acgccgaagt gcggcggcgt atcgaacagt
     1021 tgcgcgtctc gaacgtggcg gccggcggcg aggaaaccgg catgcagacc gccgtcgaga
     1081 taccgtcggt gcgctcggca agctcggcag gcacagcgag cactgccggc gccgcgaacc
     1141 aggatcagat caaaggcacc ggcgacacgc tgaataaccg cttcgtcctc gaagagtgtc
     1201 tgggcgtcgg tggaatgggc acggtgtaca aggcgctcga tctgcgcaag ctcgaagcgt
     1261 cggatcgcaa accgtatgtc gcgatcaagg tgctcaacgt ccagttccgc ggcaatccga
     1321 attcactcgt cgcgctgcaa cgcgaggcgc gcaaggcgca ggtgctcgcg caccgcaaca
     1381 tcatcgcggt gtacgacttc gaccgcgacg ggccgatcgt ctatctgacg atggagtacc
     1441 tcaccggcaa gccgcttagc cagctattgc gcacgcccgg ctataagggc atgccggttc
     1501 gcgccgcgct gccgattgtg cgcggcatgt gcaatgcgct cgcctatgcg cacgagcacg
     1561 gtttcgtgca ttgcgatttc aagcccgcca atgtctttct cacgacgaat gccgaagtga
     1621 aggtgatcga cttcggcatc gcccgcgtgt tccagcggcc ggaggaggag agcgacgcga
     1681 ctgtcttcga ccccggcacg ctcggcgcgt tgacgcctgc gtacgcgagc ccggagatga
     1741 tcgaacatcg tgagccggac ccgcgcgacg atatctacgc actcggctgc attacctacg
     1801 agttgttgac cggtcatcat ccgttcgacc ggctgtcggc gacgcaggcc cgcaattccg
     1861 ac
//
LOCUS       DATASH010000619         6585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2278762,
            whole genome shotgun sequence.
ACCESSION   DATASH010000619 DATASH010000000
VERSION     DATASH010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6585
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2278762"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1716
                     /locus_tag="VHV99_16845"
     CDS             <1..1716
                     /locus_tag="VHV99_16845"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX3637118.1"
                     /translation="RHMRRMGTGADVLTTERKLTAKETRAFMRAIASDPNVEYVEPDT
                     EMSTTMVPTDPEYFRQWSLASNLKPSTPYPGIRAEGAWDIASGSGSVIALVDNGVTSH
                     GDLDANILPGYDFTANNRGGNGSNPGITTETCSVQWHGTHVAGIMAAPANNGLGIAGI
                     ASAAKVVPVRVMNGCGKGYTSDIADGIVWAAGGSIPGVPANAHPARIINVSLGGNGIC
                     ETTFQNAIDYAVSHGAVVTAAAGNDQTSATNFEPANCRNVINVGGSNRYGARWIGSNF
                     GAVVDIAAPGDSIWSTYNSGTTSPRAEAYAFMNGTSMATPMVSAVIALAQSVAPTPLS
                     AAEMRTLLTQNAQPFPTGQPDQALGRGILDANATVVAARSRKIPAAADFRCQQSTTIM
                     QVSCTDLSTARGVPIKSWAWNFGTGAADQTRAQSVNPTVNYDYPGIYEIALTVTDNTG
                     AVSRLTRPFRVMPPAITDISPNVPTTFSASSGDMLYFALDVPAGVRSLTFRLSPGANG
                     ETGTLYLRAGTPSVLHPDCGSAWTKGANATCTLSNPASGTYYGIVSATNATVRGASVL
                     ASYTQ"
     gene            1806..3644
                     /locus_tag="VHV99_16850"
     CDS             1806..3644
                     /locus_tag="VHV99_16850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013337777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3637119.1"
                     /translation="MIFRIPRFNNDDKDVEALVARADAHFSALRMTEAIVAYQKVLSV
                     RPRDAHALHRIGLACVHTNDTEQARGYLEQALHVAPDRAELWEHAGLLAASMGNHTSA
                     EAFYQRAIRLAGSTASVHRNLGDCLRQAGRLTEAITHYEKSIDIEPGLHHAIRALARI
                     HAQLGQPDSAVHYWHRAWTLDPGSLNDGLGLITVLAKAGHTHQLADAITQMRSRFAGD
                     ATALKALAVVLNTSDRFNDALSVARQGLAIDPHHALLHHNAARALSISGRTAESRPHS
                     MEAARLLPDNPHMQFHLAGVLLALGEFEEGWKRYTWFHDLPDRRTERVRPVLREWQGE
                     TVAGCRFLLVGEQGLGDQIQFLRFAEWLHRQQATVDVLVSEPVAALASHMTGVRAAFT
                     TMPTGPYDYWCTMLRMPEHMKLDVSMLPVAMPYLTATPDKPRQWQARIEAISSGRRHA
                     ENRRVGVVWAGSPNHVLDRYRSVRLAALQPLFGLGGITWYSVQKGERERESEGLAHEY
                     DLHTLGPSINDFTDTLAILQTLDLLITVDTSVAHLAGAAGLPVWVLVPAYSEWRWLSD
                     RTDSPWYPSMRLFRQRELGEWDPVVKDVQAALQLWRDAPAAGLCVQ"
     gene            3998..4573
                     /locus_tag="VHV99_16855"
     CDS             3998..4573
                     /locus_tag="VHV99_16855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HEX3637120.1"
                     /translation="MLRVLLVTDTDKPIGDLRDALARLGYEMLAGTATPQALHRVVQS
                     ERPDVIIIDTESPSRDTLEQLAVMNATAPRPVLMFSHDANQQLIRDAVGAGVTAYLVE
                     GLSTERLAPILEVALARFAQESQLRERLAQVENELTERKLIDRAKRLLMDQQKLTEPA
                     AYASLRKRAMNQGVKLAEVARAVIASADSLK"
     gene            4584..5732
                     /locus_tag="VHV99_16860"
     CDS             4584..5732
                     /locus_tag="VHV99_16860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012402772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX3637121.1"
                     /translation="MNSASHSPSSAPACLEKTHLRLGFVALSDAAPLVAAKLLEFGHA
                     HGLTLELCRQPSWAAVRDKLLSGDLDAAHVLYGLVYGVQLGLGGPQTDMAVLMVLNRN
                     GQAITLSNRLADALAEHGTLPQALATLGRKPVFAQTFPTGTHAMWLYYWLASQGVHPL
                     RDIESVVIPPPQMVAALAEDKLDGLCVGEPWNAMAEARGVGRTVAYTSEVWPDHPEKV
                     LACRRDFVGANPNAARALVRTMLKACRWLDGAGHREDIARWLARPEFIGIDEALIAAR
                     LSDEIAGSAPQGQPVRFFDNGAVNYPRAADGAWFLTQFDRWGMIDARADYAEIAAQIN
                     QTQLYRDAAAQIGVALPGDERSHVLIDGKVWGNDTVSTAYARSFAIRA"
     gene            complement(5815..6255)
                     /locus_tag="VHV99_16865"
     CDS             complement(5815..6255)
                     /locus_tag="VHV99_16865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX3637122.1"
                     /translation="MTTTANEDITRRAIDAFYRAIEQRNVESLRKIVTPDWEYIPEPA
                     GVAPGPDQMIPVFADIAAALPDMKVTILDLLVHGDRVGVRAEISGTQTGKLLGIAASS
                     KHIHFAIHSFHQMRGNLIAKTWHLEDWLSVFRQLGQLPKTLEQH"
BASE COUNT         1264 a         2087 c         2004 g         1230 t
ORIGIN      
        1 cgccacatgc gccgcatggg caccggcgcc gacgtgttga caacggaacg caagctgacg
       61 gcgaaggaga ccagggcatt catgcgcgct atcgcatcgg accctaacgt cgaatatgtc
      121 gaacccgata cggaaatgtc gacaaccatg gttccaaccg atccggagta tttccggcag
      181 tggagtttgg cctcgaacct gaaaccttct acgccctatc cgggaatccg ggctgagggt
      241 gcgtgggata ttgccagcgg ttccgggtcg gtgatcgcgt tagtggataa cggcgtaacg
      301 agtcatggtg acctggatgc caacatcctg cccggctacg atttcaccgc taacaaccgc
      361 ggcggcaacg gcagcaatcc gggcatcacg acagagacgt gttcggtcca gtggcacggc
      421 acgcatgtcg ccggcatcat ggccgcgccg gccaacaacg gcctcggtat cgccggcatt
      481 gcttccgcgg cgaaggtcgt gccagtccgg gtgatgaacg ggtgcgggaa aggctatacg
      541 tccgatatcg ccgacggcat cgtgtgggcc gcaggcggca gtattccggg cgtgcccgcc
      601 aacgcccacc cggccaggat catcaatgtg agccttggcg gtaacggaat ctgcgagacg
      661 acatttcaga acgcgatcga ctatgcagtc agtcacggcg ccgtggttac cgcggcagcg
      721 ggcaacgatc agaccagcgc aacgaatttt gagccggcca attgccgcaa cgtcatcaac
      781 gtcggcggta gcaatcgcta tggcgcaagg tggatcggtt cgaacttcgg ggcggtggtc
      841 gatatcgctg cgccgggcga ctcgatctgg tccacgtaca acagcgggac aacttcgccg
      901 cgggctgaag cctatgcctt catgaacggc acgtcgatgg cgacacccat ggtcagcgcc
      961 gttatcgcgc tcgcccagtc tgtggcgccc acgcccctga gcgccgcgga aatgcgaacc
     1021 ctgctcacgc agaatgcaca gccctttccc accggtcagc cggatcaggc actcgggcgc
     1081 ggcattctgg atgcgaacgc aactgtcgtt gccgcgagat cccgcaagat tcccgccgcg
     1141 gcggacttca ggtgccagca gagtacgacg atcatgcaag tgtcatgtac cgacctctct
     1201 accgcgaggg gtgtgcccat caagtcatgg gcgtggaatt tcggcacggg tgccgctgat
     1261 cagacgcgtg ctcaatcggt caatccaacc gtgaactatg actatcccgg catttacgaa
     1321 atcgcgctca ccgtgacgga taacaccggc gccgttagcc gcctgacgcg ccctttccgg
     1381 gtcatgccac cggccattac ggacatcagc cccaatgttc cgacaacgtt ctcagcaagc
     1441 tcaggcgata tgctgtattt cgcactggac gtacccgcgg gcgtaaggag cctcactttc
     1501 aggctttcac ctggcgccaa cggcgaaacc ggaaccctgt atctcagagc gggcacgcct
     1561 tccgtgcttc atcctgactg cgggtccgcg tggaccaaag gtgcgaatgc gacctgcacg
     1621 ctctccaacc cggcgtcagg gacttactac ggcatcgtga gtgccactaa cgcaaccgtc
     1681 cgcggcgctt ccgttctggc gtcctataca caataagacc gcgacagcgg aatgactccc
     1741 ccgcccctgc cgcgcaccgc aggggcggct tcccgcgcac gataagcttg catcaggacc
     1801 tccttttgat atttagaatc ccccgcttca ataacgatga caaagacgtc gaggctctcg
     1861 tcgcgcgcgc ggacgcacat ttttccgccc tgcgtatgac agaggcgatc gtcgcttacc
     1921 agaaggtgct cagcgtgcgc ccacgcgacg ctcacgcgct gcatcgcatc ggactcgcct
     1981 gcgttcatac caacgacacg gagcaggcgc gcggctacct ggagcaggca ctgcacgtcg
     2041 ccccggaccg ggctgaatta tgggagcacg cgggcctgct tgccgcgtcg atgggcaatc
     2101 acacatcggc tgaagcgttc tatcagcgcg caatccgcct tgcgggcagc acggccagtg
     2161 tccacaggaa cctgggagat tgtctgcggc aagccggccg cctcacggag gcgatcactc
     2221 actacgagaa gtcgattgat atcgaaccgg ggttgcacca tgcgattcgt gctctcgccc
     2281 gtatccatgc gcaactcgga cagcccgata gcgccgtgca ctactggcac cgtgcatgga
     2341 cgctggatcc gggcagcctg aacgatggac tcggcctgat taccgtgctc gcaaaagccg
     2401 gccatacgca ccagcttgcc gatgcgatca cgcagatgcg aagccgtttt gccggcgacg
     2461 ccacggcttt gaaagcgctg gccgtcgtac tgaacacaag cgatcgtttc aacgacgcgc
     2521 tcagtgtcgc aaggcaaggt ctggctatcg atccgcacca cgcactcctg catcacaatg
     2581 cggcgcgcgc actcagcata agcggcagaa ccgccgaaag ccggccgcac agtatggaag
     2641 cggcgcggct tctgcccgat aacccacaca tgcaattcca tcttgcaggc gtgctgcttg
     2701 cgctgggtga attcgaagaa ggctggaagc gatacacatg gtttcatgac ctgccggaca
     2761 ggcgcacgga gcgagtccgg ccggtcttgc gcgagtggca aggggaaacc gttgcgggtt
     2821 gccgcttcct gctcgtgggc gaacaagggc tcggcgatca aatacagttc ctcagattcg
     2881 ccgaatggct gcatcggcaa caggccaccg tcgatgtact tgtcagtgaa ccggtagcgg
     2941 cactggcgag ccatatgacg ggcgtgcgag ccgcatttac caccatgccg accggaccgt
     3001 acgactactg gtgcaccatg ctaaggatgc cggaacatat gaaactcgat gtgtcgatgc
     3061 tgcccgtcgc catgccgtat ctgacagcga cgcccgacaa gccgcgccaa tggcaagcgc
     3121 gtatcgaagc gatttcatcg ggcagaagac atgccgaaaa cagacgcgtc ggcgtggtct
     3181 gggcaggcag cccaaaccac gtgctcgacc gttaccggtc ggttcggcta gctgcactac
     3241 agcccctgtt tgggcttggc gggatcacct ggtattcagt gcaaaaaggc gagcgcgagc
     3301 gtgaaagcga aggtctggcg catgaatacg acttgcacac gctgggcccc tcaatcaacg
     3361 acttcacgga caccctggcg atactgcaga cgctcgatct tctgattacc gtcgacacgt
     3421 cggtcgccca tctcgccggt gcggccgggc ttcccgtttg ggtgctggtc ccagcctatt
     3481 ccgagtggcg atggttgagc gacagaacgg atagcccatg gtatccgtcg atgcgcctgt
     3541 tccggcagcg ggaactgggt gagtgggatc ccgttgtcaa agacgtccag gccgcgctgc
     3601 aactgtggcg tgacgccccg gcggccgggc tttgcgttca gtgaatttgg tctcgccggt
     3661 ggcggcgccg ggctcgtggc gcggccccgg tgttgccacg ctcgttgcca cgctcgttgc
     3721 agcaccgctg ccttgctcat gttgccaagc tagcgtggcc caggcaagtc gcatcaagtc
     3781 ccgtcgagct gcacctgtcc ggtgcttgcg ctctcatgcg ccgcttcggt gcatgtccta
     3841 cgcatgcccc atgcgggtgc agcccgacgt acctcgcctg cttcactggt ttccaggccc
     3901 acggttaggc ccatgattgc cttgccctcg atgcaaccgt cctgcactgg catacacgtt
     3961 gctttatgag tagtcagccg tcgataaacc caacctcatg ctgcgcgttc tcctcgtaac
     4021 cgataccgac aagccgatag gcgatctgcg cgacgccctt gcacgacttg gctacgagat
     4081 gctggccggc acggcgacgc cgcaagcgct gcaccgcgtg gttcagagcg aacggccgga
     4141 cgtcatcatc atcgatacgg aatcgccgtc gcgtgacacg ctcgaacaac tcgcggtcat
     4201 gaacgccacc gcgccacgtc cggtcctgat gttcagtcac gatgcgaacc agcagctgat
     4261 ccgcgacgcg gtgggcgccg gcgtcacggc ctacctcgtc gaaggtctct ccacggaacg
     4321 gctcgcgccg attcttgaag ttgcgctcgc gcggtttgcg caggagtcgc agttgcggga
     4381 gcgcctcgcg caagtcgaga acgaactcac tgagcgcaag ctgatcgatc gcgccaaacg
     4441 cctgctgatg gatcaacaga agctcaccga acccgccgcc tacgccagtc tgcgcaagcg
     4501 cgcaatgaac cagggcgtga agctcgccga agtcgcgcgt gccgtcatcg cgtcggcgga
     4561 ttcgctcaaa tgaagcgccg cttatgaact ctgcctccca ctccccatcg tccgcgcccg
     4621 cgtgcctcga gaaaacgcat ctgcggctcg gcttcgtcgc gctgtccgat gccgcgccgc
     4681 tcgtcgccgc aaagctgctc gaattcggcc acgcccatgg attgacgctg gagttgtgcc
     4741 ggcagccatc atgggccgcc gtgcgcgaca aattgctgtc gggcgatctc gatgccgcgc
     4801 atgtgctcta cggtctcgtc tacggggtac aactcggcct cggcggtccg caaacggata
     4861 tggccgtgtt gatggtgctc aatcggaacg gccaggcgat cacgctctcg aatcgcctcg
     4921 ccgatgcatt ggccgaacac ggcaccttgc cccaggcttt ggcgacgctc ggccgcaagc
     4981 cggtattcgc gcagaccttt cccaccggca cccacgcaat gtggctgtac tactggctcg
     5041 cgtcgcaagg cgtgcatccg ttgcgcgata tcgagagcgt cgtgattccg ccaccgcaaa
     5101 tggtcgccgc gctcgccgaa gacaaactcg atggcctgtg cgtcggcgaa ccgtggaatg
     5161 cgatggctga ggcgcgcggc gtcggcagga cggtcgctta tacgagcgaa gtgtggcccg
     5221 atcatcccga gaaagtgctg gcgtgccggc gcgattttgt cggcgcgaat cccaatgcag
     5281 cgcgagcgct ggtgcggacc atgctcaaag catgccgctg gctcgacggt gcgggacatc
     5341 gcgaggacat tgcgcgctgg ctcgcgcgac ccgaattcat cggtatcgat gaagccttga
     5401 tcgccgcgcg gctcagcgac gaaatcgccg gctcggcgcc gcaggggcag cccgtgcggt
     5461 tcttcgacaa cggtgcggtg aactacccgc gcgccgccga cggtgcctgg ttcctcacgc
     5521 agttcgatcg ctggggcatg atcgacgccc gcgcggatta cgcggaaatc gcggcgcaaa
     5581 tcaaccagac gcagttgtat cgcgatgcgg cggcgcagat tggggtagcg ttgccaggcg
     5641 atgagcgctc tcatgtgctg atcgacggca aggtgtgggg caacgacacg gtatccacgg
     5701 cctacgcgcg gagcttcgct atccgcgcat agcgttgcgg ttgcggttag caatgcaaca
     5761 ccagcgtcaa caccaacctg tgatacctac gcgtccgggc gtgaggcgtc gtgctcagtg
     5821 ctgctcaagt gttttgggta actgtccaag ttgccggaac accgacagcc agtcctcgag
     5881 gtgccatgtt ttcgcaatca ggtttccgcg catctggtgg aaagagtgga tcgcgaaatg
     5941 aatgtgtttg gaactggcgg caatgccgag tagtttcccc gtttgagtgc cactgatctc
     6001 cgcgcgaacg cccacccggt ccccatgaac cagcaagtcg agaatggtga ccttcatatc
     6061 gggaagtgcg gcagcgatgt cggcgaacac cggaatcatc tggtcaggtc ccggcgccac
     6121 tccggcgggc tccggaatat attcccaatc cggcgtgacg atctttcgta acgactcaac
     6181 gtttctctgc tcgatggcgc gatagaacgc gtcgatcgcc cgacgggtga tatcttcgtt
     6241 ggcagttgtg gtcatgttcg gttactcctg aagaacgctg acgccaaagt ctggaccgaa
     6301 ccattctgca cgcgtgccgg catcaccttt gctagcggac attcgaggcg gtacgcatct
     6361 ggtttgttct ggagtctaca tcgacgcgca ttcgcgcgct tcgcgctcat gatcaggcga
     6421 gtggtttgcg aaaaacgctc ccgccataac cgagcacgat attgtcgact tcgaacggtg
     6481 acgcctcctg cgcctcgagg atgaacgcga cgcggccggg agtcagtttg ctggatatcg
     6541 aggcggccag ttcggcgtcg agcgtgtcct tgatcgcatg ctcag
//
LOCUS       DATASH010000620         6041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_496761,
            whole genome shotgun sequence.
ACCESSION   DATASH010000620 DATASH010000000
VERSION     DATASH010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6041
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496761"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VHV99_16870"
     CDS             complement(<1..238)
                     /locus_tag="VHV99_16870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637123.1"
                     /translation="MLRVLAPFLLHVRPVRSGSAVRLVRRVFFLRRGGRAFHSASLLA
                     RRFRRVAFSIRRITAPRVAGLHLIGGRRRILVRRS"
     gene            206..4579
                     /gene="hrpA"
                     /locus_tag="VHV99_16875"
     CDS             206..4579
                     /gene="hrpA"
                     /locus_tag="VHV99_16875"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012400882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HEX3637124.1"
                     /translation="MKQEGRKHAQQGDAAHKQPDGAAQPARRHDRRAQKAPQGQSQPH
                     GEARRAHPDGARAPNQPKRGEADAASNAQRAARANQQSGAPVQNGQPRREPGGSAQQS
                     PQAGAAQNDPPRREPRPPRVQRVVEPNPIPPITFPEALPVSGRREEIAKAIAQNQVVI
                     VCGETGSGKTTQLPKICLELGRGLGAGGAGLIGHTQPRRIAASATGRRIAEELGTPFG
                     EVVGYKVRFTDNLSPGASVKLMTDGILLAETQTDPLLKAYDTLIIDEAHERSLNIDFL
                     LGYLKEILVKRPDLKLIVTSATIDADRFARHFGSEEKPAPVIEVSGRLYPVEVRYRPV
                     AEDSPAVKSAEGSATASSRESRDRPKTQRETDRDLMEAIVDAVDELCREGPGDVLVFL
                     PGEREIRDAAEALRKHHPPHTEILPLFARLSAAEQERVFRTSNARRIVLATNVAETSL
                     TVPGIRYVVDTGLARVKRYSYRNKVEQLQVESISQAAANQRAGRCGRVADGICIRLYE
                     ESDYQGRVRFTDPEILRSSLASVILRMKSLHLTAIETFPFIEPPPGRAIADGYQLLNE
                     LGAVDDDNQLTPLGRELARLPLDPRVGRMILAARDQQALKEVLIIASALSVQDPRDRP
                     IEAQEQADQAHRRFADERSEFLQWLKIWNWFEEAIAHKKSNRQLQDECRKNFLSQLRL
                     REWRDVHSQLLTVVREHGWRLNEAEATFEQIHLALLTGLLGNIGLKADDEPYFLGARS
                     IKFYLWPGSALVKKAGKWVMAAELVETSRLYARCIAKIEPEWIEKIGEHLLKKSLSEP
                     HWEKRAAQVSAFERAMLYGLPIYHRRRVSFGKQDPARARELFIRGALVEGEFDTKLAF
                     FAHNRKLLADIEQLEHKSRRQDVLVDDELIFGFYDQALPQGIYTGASFERWYRDEVKK
                     SAQPEDKLRLLYLSRDDLMRHEAAGVTTDLFPKRMTMAGVEMALTYHFEPGSPRDGVT
                     LAVPLYALNQVDARRCEWLVPGMLKEKTQLLLKSLPQKLRRHCVPLPEYAAGFVERHS
                     GPRFGAGGLLESLIADVREQTQVAMKQSDFKLETLTPHLFMNFKVVDEHGRQLAMGRN
                     MSQLRAELGGQAQQHFQKIASGAAGAALADAGGGGGVAGAAQASGASGAHAGGAASGA
                     ASRGKGAAASGPLTALQDGAQGTAQGTALYEKLTTWNFGKLPELLEIRRGGQTLFGYP
                     ALVDRGTHCDVEVFDSPDEAARIHRAGLRRLFALQLKEPIKYLEKNLPGLREMAMQFM
                     PRGTQEELRDQLIDTALDRACLQDPLPADDVSFHTRRDEGRSRLTLLAQEISRLVGQI
                     LSEYASATKKLVQAKSFTAAHADMQNQLDGLIGKRFVVDTPYPQLAHFPRYLKGIALR
                     VDKLKADSTRDARQFAEFHPLLQNYQRAVAQRGGVLDPRLSEFRWLLEELRISLFAQE
                     LRTPMPVSVKRLYKVWESMQR"
     gene            complement(4816..6041)
                     /locus_tag="VHV99_16880"
                     /pseudo
     CDS             complement(4816..6041)
                     /locus_tag="VHV99_16880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588953.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="YadA family autotransporter adhesin"
BASE COUNT         1079 a         1917 c         2022 g         1023 t
ORIGIN      
        1 cgctgcggcg aacgagaatg cggcgccggc cgccgatcag gtgaagcccg gcgacgcgcg
       61 gcgccgtgat gcgccggatg ctaaacgcaa cgcgccggaa acggcgcgcg agcagcgacg
      121 cgctgtggaa ggcgcggccc cctctgcgca gaaaaaaaac ccgccgcacg aggcgcaccg
      181 cggagccgga gcgcacgggg cgcacatgaa gcaggaaggg cgcaagcacg cgcagcaagg
      241 agatgcggcg cacaagcagc cggatggcgc ggcgcagccc gcacgtcgtc acgataggcg
      301 cgcccagaaa gcgccccaag gccaaagcca gccgcacgga gaagcgcggc gcgcgcatcc
      361 ggacggcgcg cgggcgccga atcagccgaa gcgtggcgaa gctgacgccg catcgaatgc
      421 acagcgcgcg gcgcgtgcca atcagcaaag cggcgccccg gttcaaaacg gtcagccccg
      481 tcgcgaaccg ggcgggtccg cgcaacaatc gccgcaagca ggtgctgcgc aaaatgaccc
      541 gccccgccgc gaaccacgcc ctccgcgcgt tcagcgcgtc gtcgaaccga atccgattcc
      601 gccgatcacg ttccccgaag cgctgcctgt ctcgggccgc cgcgaggaaa tcgccaaggc
      661 catcgcacag aaccaggtcg tgatcgtctg cggcgaaact ggctccggta aaacgaccca
      721 gttgccgaaa atctgcctcg aactcgggcg cggtctcggc gcgggcggcg cgggtctgat
      781 cggccacacg cagccgcgcc ggatcgcggc atcggcgacg ggccgtcgca tcgccgaaga
      841 actcggcacg ccgtttggcg aagtggtcgg ctacaaggtg cgcttcaccg acaatctgtc
      901 accgggcgcg tcggtcaaac tcatgaccga cggcatcctg ctcgccgaaa cgcagaccga
      961 tccgctgctc aaagcctacg acacgctgat catcgacgaa gcgcacgaac gcagcctcaa
     1021 catcgatttt ctgctgggct atctgaagga aattctcgtc aagcgccccg atctgaagct
     1081 gatcgtgacc tcggcaacga tcgacgcgga tcggttcgca cgccatttcg gcagcgaaga
     1141 aaagcccgcg ccggtgatcg aagtgagtgg gcgtctgtat ccggtcgagg tgcgctaccg
     1201 gccggtcgcg gaggacagtc cggcggtgaa gtcggcggaa ggctcagcaa cggcgtcgtc
     1261 acgtgaatcg cgtgacaggc cgaagacgca gcgcgagacc gatcgcgatc tgatggaagc
     1321 gatcgtcgat gcggtcgacg aactgtgccg cgaaggcccc ggcgacgtgc tggtcttcct
     1381 gcccggcgag cgtgaaatcc gcgatgccgc cgaagcgttg cgcaagcacc atccgccgca
     1441 cacggaaatt ttgccgctgt tcgcgcgttt gtcagcagcc gaacaggaac gcgtgttccg
     1501 cacctcgaac gcgcgccgca tcgttttggc gaccaacgtc gcagaaacct cgctgacggt
     1561 gccgggcatt cgctatgtgg tcgatacggg tctcgcgcgc gtgaagcgct actcgtatcg
     1621 caacaaggtc gagcaactgc aggtcgagtc gatctcgcag gccgccgcga atcagcgggc
     1681 agggcgttgc ggccgcgtcg cggacggcat ctgtattcgt ctgtacgaag aaagcgacta
     1741 tcagggccgc gtgcgcttca ccgacccgga gattttgcgc tcgtcgctgg cgtcggtgat
     1801 tctgcgcatg aagtcgctgc atctgacggc gatcgaaacc ttcccgttca tcgagccgcc
     1861 gccgggccgc gcgatcgccg acggttacca gttgctcaac gaactgggcg ccgtcgacga
     1921 tgacaaccag ctcacgccgc tcggtcgcga actcgcgcgc ttgccgctcg atccgcgcgt
     1981 cggccggatg attctcgccg cgcgcgatca gcaggcgttg aaggaagtgc tgatcatcgc
     2041 cagtgccttg tcggtccagg atccgcgtga ccggccgatc gaagcgcagg agcaagccga
     2101 ccaggcgcat cgccggttcg ccgacgagcg ttcggaattc ctgcaatggc tgaagatctg
     2161 gaactggttc gaagaagcca tcgcgcacaa gaagtcgaac cggcaattgc aggatgagtg
     2221 ccgcaaaaac ttcctgtcgc aactgcgcct gcgtgaatgg cgcgacgtgc attcgcaact
     2281 gctgacggtg gtgcgcgagc atggctggcg gctgaacgag gccgaagcga cgttcgagca
     2341 gatccacctc gcgttgttga ccggcttgct cggcaacatc ggcctcaaag ccgacgacga
     2401 accgtatttc ctcggtgcgc gcagcatcaa gttctacctc tggccaggct cggcgctggt
     2461 gaagaaggcc ggcaagtggg tgatggccgc ggaactggtc gagacgagcc gcctgtacgc
     2521 gcgctgcatc gcgaagatcg agccggaatg gatcgagaaa atcggcgagc atctgctgaa
     2581 gaagtccttg tccgagccgc attgggaaaa gcgcgcggcg caggtgtcgg cgttcgagcg
     2641 cgcgatgctg tacggcctgc cgatctatca ccggcggcgc gtgagcttcg gcaagcagga
     2701 cccggcgcgg gcgcgcgagc tgttcattcg cggcgcgctg gtcgaaggcg agttcgatac
     2761 gaagctcgcg ttcttcgcgc ataaccgcaa gctgcttgcc gatatcgagc agctggaaca
     2821 caaatcgcgc cgccaggatg tgctggtcga cgacgaactg attttcggct tctacgacca
     2881 ggcgttgcca caagggattt acacgggtgc atcgttcgag cgctggtatc gcgacgaggt
     2941 gaaaaagagc gctcagccgg aagacaaatt gcgtctgctg tatctgtcgc gcgacgatct
     3001 gatgcgacat gaagcggccg gcgtgacgac cgatctgttc ccgaaacgga tgacgatggc
     3061 cggcgtcgag atggcgctga cctatcattt cgagccgggt tcgccgcgcg acggcgtgac
     3121 gctcgcggtg ccgctgtatg cactcaatca ggtcgacgcg cggcgttgtg agtggcttgt
     3181 gccgggcatg ctgaaggaga agacgcagct tctgctgaaa tcgttgccgc agaagctgcg
     3241 gcgtcattgc gtgccgctac ctgaatacgc agccggtttc gtcgaacggc atagcggtcc
     3301 gcgcttcggc gcgggcggct tgctcgagtc gctgatcgcc gatgttcgcg agcagaccca
     3361 ggtcgccatg aagcaatccg acttcaagct cgagacgctg acgcctcatc tgttcatgaa
     3421 cttcaaggtc gtcgacgaac acgggcggca actcgcgatg ggccggaata tgtcgcaact
     3481 gcgcgcggaa ctcggcggcc aggcacagca gcattttcag aagattgcgt cgggcgcggc
     3541 tggagcagcg ttagcggatg cgggcggcgg tggcggcgtg gctggtgccg cgcaagcgag
     3601 cggcgcatcc ggcgcgcacg cgggcggcgc ggcaagcggt gcggcgtcgc gaggcaaggg
     3661 cgctgctgcg tccggcccgc ttaccgcgtt gcaagacggc gcgcaaggca ccgctcaagg
     3721 caccgcgctg tacgaaaagc tgaccacatg gaattttggc aagctccctg agctgctcga
     3781 aattcgccgt ggcgggcaga ctttgttcgg ctatccggcg ctagtggatc gcggcacgca
     3841 ttgcgacgtc gaagtgttcg attcaccgga cgaggccgca cgaattcatc gcgcagggct
     3901 gcggcgattg ttcgcgttgc agctgaaaga gccgatcaag tatctggaaa agaatctgcc
     3961 gggcctgcgc gaaatggcga tgcagttcat gccgcgcggc acgcaggaag agttgcgcga
     4021 tcaactgatc gacaccgcgc tcgaccgcgc ctgtctgcaa gacccgttgc ccgccgacga
     4081 tgtcagcttc catacgcgcc gcgacgaagg ccgcagccgt ctcactctgc tggctcagga
     4141 aatctcgcgg ctggtggggc agatcctgag cgaatacgcg agcgcgacga agaagctcgt
     4201 gcaggcgaag tcgttcacgg cggcgcatgc ggacatgcag aaccagctcg acgggctgat
     4261 cggcaagcgc ttcgtggtcg atacgccgta cccgcagctg gcgcactttc ctcgctatct
     4321 gaaagggatt gcgctgcgcg tcgacaagct gaaggccgac tcgacgcgcg acgcgcggca
     4381 gttcgctgag tttcatccgc tcctgcaaaa ctatcagcgc gcagtggcgc aacgcggcgg
     4441 cgtgctggat ccgcgcttat cggagttccg ctggctgctg gaagagttgc ggatttcgct
     4501 gttcgcgcag gagttgcgca cgccgatgcc ggtttccgtg aagcgtctct acaaggtgtg
     4561 ggagtcgatg cagcgctgag tggcgagtgg catggaggcg ccttgcactt gcttcattcg
     4621 agcggcgttg accggcttcg tgcaacgctt ttgtcgcgcg cacaaaaaag cgcgcgatac
     4681 cactccttgt gagaatgaca tcgcgcgcaa aggacgcgga cactcccgaa tccgcgcccg
     4741 ctcgctgcta tacaactcct tcagtagcct tcaacgtgtc tggctcaggt tgagccagac
     4801 acgcgtgttg acgtcttacc agtgcatgcc ggcaccgacc gacgcgccga actgaccacg
     4861 cgagtcggtc gtaccttgca ccttgtagac ccacttgccg gtttacgaca gttgcgacac
     4921 accgatggcc atcgccgact ggccgccgta cgtgccgccc gcgatcgcca ccatgccgtg
     4981 acccggcaac accgcttgcg gcaagccggc aaccgccagc gccgacgccg tgccgccgta
     5041 tgcatcgcgg cgggccgagc ggatctggtc gtcggtgtag ctgttggcct ggctgactgc
     5101 gccgctcacg ctttgttgca actggttcac gttgaccgcg tccgtgccct gtacgccggc
     5161 ggcgacattg gtgatctgac gctcctgacc tgctgcgcca accgataccg tattcgcccg
     5221 atccgcgacg gagccctggc caagcgcgac cgaattgtcg gcgctcgcat ttgccgcggc
     5281 gccgagtgcg gtggcgttgt tgccgctcgc gctcgaattc gcgccgattg ccaccgcgtt
     5341 gctgcccgtg gcttgcgcgt tcgcgcccga cgcgaccgag tgcgtgccgg ttgccgccgc
     5401 gccttcggta tcgcgatcgc cttccgcgct gtagaacgga ttggctgtat tgaccgtgat
     5461 gttgctgacc gcgccgttga tggcggcatt cagctgaccg aggttgaccg catcgcttgc
     5521 ttcgacgccg tcggcgacgt tgtgaatcgc ggtgcctgcg tcgccgccga gcgtcacgct
     5581 gttcttgttg acgctgccat cggcgttctg gtcgtacatc acggcgccgt cgagcttggc
     5641 ggcagtggcg gccgtctggt cgctgaccgc cttcagctgc gagacgttga cggcgtcggt
     5701 gtcggcggtg ccggctgcga cgttggtgat ctgacgctcg ctgcccgccg agccgaccga
     5761 aacgctgttt gcgcggtctg cgatcgagcc ttggccgagc gcgacggagc cggcgacaac
     5821 ggcgctcgcg ccttggccca aggcaaccga gccgttggcg ctggcttcag ccatgttgcc
     5881 gagcgcgacg ctgccgaatg ccgtcgcgga ggcgttgctg ccgattgcgg tggcgagcgg
     5941 gccagtggct gttgcgccgc cgcctaccgc cgtgtcgccg tcgtcgctgg cgacggcgtt
     6001 ggcgccgacc gctgtcgaaa tcgtgccggt ggcggtggcg a
//
LOCUS       DATASH010000621        11792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2673584,
            whole genome shotgun sequence.
ACCESSION   DATASH010000621 DATASH010000000
VERSION     DATASH010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 11792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11792
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2673584"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VHV99_16885"
     CDS             <1..222
                     /locus_tag="VHV99_16885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011402526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX3637125.1"
                     /translation="PSPPTCRRRFDEWQADGTLAEIVKRLGTSGREISLRGRIGATAA
                     KPPAPPSRDRLRGAFWTNPESWRAPVKMA"
     gene            517..771
                     /locus_tag="VHV99_16890"
     CDS             517..771
                     /locus_tag="VHV99_16890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637126.1"
                     /translation="MKPMSLLLCGIVVSSMAAPALAQQRPQGWNWRPDHTATAEAWQQ
                     SETRGRGFTPPAPRGDLRGDIASNVRTRPEPPRESPTRRQ"
     gene            complement(1149..1472)
                     /locus_tag="VHV99_16895"
     CDS             complement(1149..1472)
                     /locus_tag="VHV99_16895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637127.1"
                     /translation="MTTYSNEAVLEALRRAQYRQVPWARRPGVFQYLRALGLMDTVRQ
                     RTVAPAPGFHAPVDIAVLTERGRAEFTRLAHDEGLLTWSAQRMNDYVVATAETRPTAA
                     LEARP"
     gene            1740..2459
                     /locus_tag="VHV99_16900"
     CDS             1740..2459
                     /locus_tag="VHV99_16900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEX3637128.1"
                     /translation="MILFTLLALFFAAIVLWKRARRPLAICTIALFWLLAAGWLTAPL
                     LELAQPHRQTDSPASFAPRTAIIMLGGGTIYDNDDVLVPPRDVLARIAVSAENYAACK
                     RTAATCQVIVSGGNPQRHSATEADTYLPYLLRKQVPRADIVLENSSRTTYENARNVSA
                     ILDRSRYDSLILVTSAYQMPRALLDFHRFGLAPQPLVSSARRARLGVLPRYDNLVNAE
                     IALHELVGIAQFHVYCAIGWF"
     gene            2706..2945
                     /locus_tag="VHV99_16905"
     CDS             2706..2945
                     /locus_tag="VHV99_16905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637129.1"
                     /translation="MKKVSLAILVSLSAVTGAAYAQDNGVTMSTDPAKAADVEQRAQD
                     LQNRQQAMQSAPMPEKHHKMAPHHKKAAKPMAASS"
     gene            3145..3402
                     /locus_tag="VHV99_16910"
     CDS             3145..3402
                     /locus_tag="VHV99_16910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEX3637130.1"
                     /translation="MSNIQLDIEWTEAASRKIEKLMPRGGQEAFLALPPVECLPMEGD
                     VLFLGPDGKQQPFIVAERQYHHDGDADWTIILILDVPQATH"
     gene            complement(3506..3797)
                     /locus_tag="VHV99_16915"
                     /pseudo
     CDS             complement(3506..3797)
                     /locus_tag="VHV99_16915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018418372.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
     gene            4370..5665
                     /locus_tag="VHV99_16920"
     CDS             4370..5665
                     /locus_tag="VHV99_16920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018418373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX3637131.1"
                     /translation="MPTVRELTLSGLLPHLVRDESGWTATWRALTLHSVFQPVLSVTH
                     QCVVGYEGLLRAFDPVGLPVSPEVLFSGTRSAADARELDRIARCLHVANFMEQGISTG
                     WLFLNTRPQVFETGWPQRTFIDELSAHFGLPQERIVIEVLEQPADDESAVASMLAASQ
                     PRDFLIAIDDFGTGFSNFDRVWRFRPDIVKLDRSLVARAGKREGDDSMISHLIAMLHQ
                     SGTLVLAEGVETDEELMILMQADVDFVQGFWLGQPKSSVQAACAKVPTLIESMWTKFA
                     AYERAHAGHQRLGFEGFAEAVLAAADTFKATGDLRQAAQKIWHLPEARRVFITDGRGE
                     QTQASVTAAAVPPPPLRLAPLYTDTHSNWSRRAYFKHALAAPGRVAMMGPHYSLADGQ
                     DCYTAALAFERDGTHVLCVDFVPTTSPADARLGARGSKR"
     gene            complement(5809..6213)
                     /gene="arfB"
                     /locus_tag="VHV99_16925"
     CDS             complement(5809..6213)
                     /gene="arfB"
                     /locus_tag="VHV99_16925"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEX3637132.1"
                     /translation="MTSRYSIPPNEIELTAVRAQGAGGQNVNKVSSAIHLRFDVQASS
                     LPDVLKMRLLALSDHRLTRDGVVIIKAQEHRTQEMNRAAALARLDELIESVSVTRKPR
                     VATRPTRASNRRRLDSKAKRGEIKSGRGRVED"
     gene            6365..7006
                     /locus_tag="VHV99_16930"
     CDS             6365..7006
                     /locus_tag="VHV99_16930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011853312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VC0807 family protein"
                     /protein_id="HEX3637133.1"
                     /translation="MKLRPGFVLELAVNFLLPWLAYRLALPHLGETGALMASAVPPIV
                     WSLIELLRFRRVDALSVMVVAGIVLSVAAMALGGSPRMLLLRESLVSGAVGVVFLLSL
                     PMRRPLIFYLARATVAREMEGGAERFEALWRERPALVGAMRLMTLVWGVGLTGETALR
                     AWMALTWPIERFLVVSPFIGYGIYGALTLWTLWYRKTMRSRVEAHVQPGGIAG"
     gene            complement(7140..9488)
                     /locus_tag="VHV99_16935"
     CDS             complement(7140..9488)
                     /locus_tag="VHV99_16935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3637134.1"
                     /translation="MSTFERISSAPPELDDIGVNLPAPADFSTALAEGFARRIGTLAR
                     RGGASGEAVKWAARAAFAASRATAEGHVCLPLAVLARRFDASSAEVRVALFASGMASD
                     GSPGAAALRPLVIDGQGRLYLARYYDYERRLARSLVAHAGGWAGAGGSAETQADAGEG
                     GNANGSAASRVGADVAAGAAAGAVADVGAGASADAGAGADAGADAGADAGAGAGAGAG
                     AGAGADANANADTTAQTLHARLLRYFGPPQDDEVDWQRVAAVMALSGRLTIVSGGPGT
                     GKTTTVVGVLACLLDARPDLRIALAAPTGKAAQRMQEALLARAGDLPPELAARLPRTS
                     YTLHRLLGSGPGGRFRHHRDNPLPYDVVVIDEASMIDVAMAAHLLDAIAPQTRLVMLG
                     DKDQLAAVEAGAVFAELSARPAFTPNGLQRIAAALGIEAARLSQALPDASNGFDEGPD
                     DRFGYTHAPGDFDTRPGDFFVDADAGGVFDGLPGDDLFASGTVPTAGAPRPSPSPVQN
                     PLADCVVWLERNYRFGLESPIGRLSLAIRKGAAAAALDVLRIDPGEPCAAALHEDTHA
                     TLADRTVARLAAGFAPYADALVEALAADTPDPAPLFDALNRFRILCATRSGPRGVDQV
                     NAAMATQVRRSAGVTLAVGAQWFAGRPVMVTRNDYALGLFNGDIGIALPGAGGALRVY
                     FRTGGGGLRAVSPAALPPHDTAFALTVHKSQGSEFQHAVLMLPSVFSRVLSRELVYTA
                     ITRARERVEVIGARAVLAQAIETPTQRDSGLAARIGEAWRVA"
     gene            complement(9485..>11792)
                     /locus_tag="VHV99_16940"
     CDS             complement(9485..>11792)
                     /locus_tag="VHV99_16940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX3637135.1"
                     /translation="PIVEACNRLFEANPQAFVLDGLDYQPVRAGERRRPPFSEPEANA
                     GDFRVWTLPQGDAAFTKREAQRAASEACAAEIVRLLRGARGGSVTIGDAPLAPGNIAV
                     LVQTHKQGSLIKRVLALWGVGSVELAQASVFATLDAEQIERVLAAVDTPGDLRRLRAA
                     LATDWLGLDAAALWRLEQVADAPAPADDPARAADAMGWVERFSRYRTLWHERGFALMW
                     RTLMRELRVAQRLVAGPDGERRLTNVNHLAELVQARAATQPGIAPTLRWLAAQRTQGG
                     GEDAQLRLESDRNLVQIVTVHKSKGLEYAVVFCPFLNDGALREPPSSGLPDAREYHDE
                     TGEAVLHYGCDDDEAERASRYAVREQAAERARLVYVALTRAVYRCYLVAGTYLSSRST
                     RESRRSVLNWLVGGGGHSFGDWLTDPPDEAALSERWRALAGGPITLQALPKVERRVPL
                     ESLQDSGTRMHARASRRPLRDQWRMASFSGLIAAGSKAEEAHTAVEDVRPDHDEIADA
                     LAPAPLAVPQAPSYAEDDILAFPRGAAAGDCLHRMFELADFTAPHTWPDAIRGALRER
                     PAPAVPELAARLAAMMHNLIADVVTTELVPGMALARLNPRRRLNELEFLFAAPSLDFP
                     ALRELLIEHGYPDVALEPGVLRGFVKGFIDMIVEHDGRFWIVDWKSNHLGDTAADYAA
                     APLESAMASHAYHLQALLYTVALHRYLKTRVRDYAYETHIGGYLYLFVRGVRPQWRDA
                     DGPAGVHMRRPAFELVALLDAAMIGGVA"
BASE COUNT         2072 a         3990 c         3633 g         2097 t
ORIGIN      
        1 ccgtcgccgc cgacttgccg ccgtcgcttc gacgaatggc aagccgatgg tacgctcgcc
       61 gaaatagtta agcgactcgg cacgagcggc cgcgaaattt cgctgcgcgg ccgcattggc
      121 gcaacggcgg cgaagccgcc tgcaccgccg agccgtgacc gcctgcgtgg tgcgttctgg
      181 accaatccgg aatcgtggcg cgcgccggtc aaaatggcct gacgtgaatt gttttgccgc
      241 cgggcgcctg ggggcgcccg gcgcatgtct gggccgccgt gcgccgtatg tcgacgctca
      301 cccgacccgc catgggcacc gctccccggc gcgcccagcg tagtcgtccc ttccccttcg
      361 ccgacttcat cacgcaccgc ttcagacgcc tgaagcggac cgatgtttcc ggtgaaacat
      421 ccatttacca ttacttacag cgtgctttcg ctactcggca taccttatgg tgtatgcaaa
      481 caggccttga gtcgatgggc ttgacgggag ctcagcatga aaccaatgtc actgcttctg
      541 tgcggcattg tcgtttcatc aatggccgca ccggcgctcg cacagcagcg cccccaaggc
      601 tggaactggc ggccggatca tacggccacc gctgaagcct ggcagcaaag cgaaacacgc
      661 ggccgcggct tcacgccgcc ggcgccgcgc ggcgatctgc gcggcgatat tgccagcaat
      721 gtccgcacac ggccggaacc gccgcgcgaa agtcccacgc gccggcaata gcattgacga
      781 cgcgccgatg gcttgttggg cgcgctcgga tcgtgctctg aatcgcgtgc atcggcgatg
      841 gccgccgttg gcggccatcg cttgcgttca gcgcaagcgc ggaggaatgc gaagcatggg
      901 gcattgaacg cccggggaac cacctggcgc tacgccagtc tcatttaacg ccattagcac
      961 agcgtggcag tagtaattcc ggtatgcccg tatcctcgcg atacgggctt ttttttgctt
     1021 gttcgctaca aggagtgaac agcccacgca tcgcactcac gtatctaagc tgagccatca
     1081 ggcggagcaa taaaatcacc gccagccgcg tcaagcggcc ggaaaagcaa cgccagctag
     1141 ccccgttcct agggccgggc ttcgagagcg gctgtgggcc gcgtttcggc tgtggcgacg
     1201 acgtagtcgt tcattcgctg cgcgctccag gtcaacaggc cttcgtcgtg cgcgagcctc
     1261 gtgaactctg ctctgccacg ctcggtgagc acggcgatat cgacgggcgc atgaaagccc
     1321 ggagccggcg cgacggtgcg ctgccgaacg gtgtccatca ggcctaacgc gcgaagatat
     1381 tggaaaacac ccggccgtct ggcccatggg acctggcgat attgcgcccg ccgcagcgct
     1441 tcaagtaccg cttcgttgga atacgtggtc atctcacttc tttcttaaag tgcagctatg
     1501 acacatctat gcccaacacg tcaccgcgcc accagtgacg cgcctggcac ccgcccttgg
     1561 ctggaagctg gctgtcttga cagacagccg aattccacca ggtgctgcct ggtctggttt
     1621 tcggaacccc cgtctgaacg gcatgttagc gttctgtcca cgggccgccg atgcaaccgc
     1681 tgcggtgcga tagcaagccc gtagatttat cccgaaagtc atacgttacc ccatttaaat
     1741 tgattctatt cactttatta gcgctttttt ttgcagcaat cgtgctatgg aaacgcgccc
     1801 gccgaccctt ggcgatttgc acgatcgccc tgttctggct gctcgcggcc ggctggctga
     1861 ctgctccatt gctcgaattg gcgcaaccgc atcggcagac tgactcgccc gccagcttcg
     1921 cgccgcgcac cgcgatcatc atgctcggtg gcggcaccat ctacgacaac gacgatgtac
     1981 tggtgccacc gcgtgacgtg ctagcgcgca tcgcggtgag cgcggaaaac tatgcggcct
     2041 gcaagcgtac ggcagccacc tgtcaggtga tcgtgagcgg tggcaatccg cagcggcata
     2101 gcgcaaccga agctgatacc tatctgccgt acctgctgcg caaacaggtg ccgcgtgcgg
     2161 acatcgtgtt ggaaaacagc agccgcacga cttacgagaa cgcgcgcaac gtctcggcga
     2221 ttctcgaccg atcacgttac gattccctga tcctcgttac ctccgcttac cagatgccgc
     2281 gcgccttgct cgacttccat cggttcggcc tcgcgccgca acccttggtc tcgagcgcgc
     2341 gccgcgcacg tcttggtgta ctgccgcgct atgacaatct ggtgaatgct gaaattgcgc
     2401 tgcacgaatt ggttggaatc gcacaattcc atgtctattg tgcaatcgga tggttctgaa
     2461 tctcacacgc cgtgctatgg caatttattt agagatgcga atattctttg tgtcggcagc
     2521 acgttagtca tgcttgatcc gagaccgcta tcgacagtga ctgtggggcg gacgtaacaa
     2581 gatgtaacga tttgtcatta ccgttacaaa acgtccgcta gagcggatat tgctggctag
     2641 aatgcattgt ctttccgatt ggacaggcaa gtacttgggt ccaccatcac tctgctgagg
     2701 taacaatgaa gaaagtgtcc ctggcgatcc tggtttccct cagtgccgta acgggcgcgg
     2761 cctatgcgca ggacaacggc gtcacgatga gcaccgatcc ggccaaggcc gccgacgtcg
     2821 aacagcgtgc gcaagatttg caaaaccgcc agcaagcaat gcaaagcgcg cctatgccgg
     2881 aaaaacatca caagatggcc ccgcaccaca agaaggccgc caagccgatg gccgccagtt
     2941 cgtaagcgca gcgacagcat caccccgcgg ccacgccgcg aaggtgaaaa gccgaaggcg
     3001 gcaccgccgg tttcggcttt tttgttgtct aatggacttg ctacaggccc gctgcgcccg
     3061 gctgacgtta tgctaaagtc gcgcaccgac cggcgcccgg ccggactccc cctaacctgt
     3121 gcgcaccttg gtgcggagga cagcatgagc aacatccagc tagatatcga atggactgaa
     3181 gcagcatctc gcaaaatcga aaaactgatg ccgcgcggcg gtcaggaagc gttcctcgcg
     3241 ctgccgcccg tcgagtgcct gccgatggaa ggcgacgtgc tgtttctcgg cccggacggc
     3301 aagcagcaac cgttcatcgt cgcggagcgc cagtatcacc acgacggcga tgccgactgg
     3361 acgatcatcc tgattctcga cgtcccgcaa gccacgcatt gaagataaaa cggcgagtgc
     3421 cgcgcaggcc ggatccggcc tggcggttcg ctcgccgtgg ttcgttcggc ccgggttgtt
     3481 cggcccggcg attcgccctg aacgctcacg cgatcttcga cacgacgcga atttccgcgt
     3541 gttcgaccca atcccgccac cgctttccta taagccgcaa tgtgcgcgga tccggcatgc
     3601 gccgccagca cttcctggct ttccccacgt tcgacaaaca cgaacctgcg cggctcctgc
     3661 acgtcacgat gcagatcgta ttgaagcgca ccctgctcct tgcgcgtcgg cgcggcgatg
     3721 actttgagcg cttcgcgcag ttgctgttca tagcccggca ttgccaccga gattgcgacc
     3781 accgcgactt cggccatgcc tgtgcgcagt ttattcacca aaagcggcag catacgtcgc
     3841 ccgccgaacc tgcaggcaag cgcgtgcgcc gagcagagca ggctcgcacg gcggcggttg
     3901 agctggtctg aggaaaggtc cgcgcacgcg aaacgtctga gcaccgtcct tgcctggagt
     3961 ttggcgggcg gccactcacc gccctgctac cctcggcttc atacgtcgcg attgattgcg
     4021 tttaaaacag tcgggcgtgc ccgtgagggt actcgaaggc gggcgcgact ccacacgcga
     4081 cgcgcgcgac atcgaggctg gctacccgct gtggccgctt tgccgggcac ctcgccacgt
     4141 cccgcgtttg tgataacccg cacattcatg cgaagtgcga actgctagac tggttttgac
     4201 taattccgcc ggagtccagc atggctggca ttgctctccg cgtttccggg ctgtgcccgc
     4261 ctcaccctgc cgccgcctgg gctgtgcatc cgctgcgcgc cgaatctgtt tgcgcgcccc
     4321 gtcacccgtt tgccgcgatg tcaccatgcc cgctctcatg cctgccctta tgccgactgt
     4381 cagagaattg accctgagcg gcctgctccc gcatctggtc cgtgacgagt ccggctggac
     4441 cgcgacgtgg cgcgcgctga ccctgcatag cgtgttccag ccggtgctgt ccgtcacgca
     4501 tcagtgcgtg gtcggttacg agggcttgct gcgcgcgttc gacccggtcg gcttgccggt
     4561 ctcgccggaa gtgctctttt ccggcacccg ctcagccgcc gacgcgcgcg aactcgaccg
     4621 catcgcacgc tgcctgcatg tggcgaattt catggaacag ggcatcagca ctggctggct
     4681 gtttctgaat acgcgtccgc aggtattcga aaccggctgg ccgcagcgca ccttcatcga
     4741 cgaactctcg gcgcatttcg gcctgccgca ggagcgcatc gtcatcgaag tactcgaaca
     4801 acccgccgac gatgaatccg ccgtcgccag catgctcgcg gcgtcgcagc cacgcgattt
     4861 cctgattgcg atcgacgatt tcggcaccgg tttttcgaac ttcgaccggg tgtggcgctt
     4921 tcgccccgac atcgtgaaac tcgaccgctc gctggtcgcg cgtgctggca agcgcgaagg
     4981 cgacgattcg atgatcagcc atctgatcgc gatgctccat cagtccggta cgctggtgct
     5041 ggccgaaggc gtcgaaactg atgaagagtt gatgattctg atgcaggccg acgtcgattt
     5101 cgtgcagggt ttctggctcg gccagccgaa gagctcggtg caggcagcgt gtgccaaagt
     5161 accgacgctg atcgagtcga tgtggaccaa gttcgccgcc tatgaacgcg cgcatgccgg
     5221 ccaccagcgg ctcggcttcg aagggttcgc cgaagccgtg ctcgccgctg ccgacacctt
     5281 caaggcaacc ggcgatctac ggcaggcggc gcaaaagatc tggcatttgc ccgaagcgcg
     5341 ccgggtattc atcactgacg gccgaggcga acagactcag gcgtccgtga ccgccgccgc
     5401 ggtgccgcct cctccgctgc ggctcgcgcc gctctacacg gacacgcaca gtaactggtc
     5461 gcggcgtgcg tatttcaagc atgcgcttgc cgcaccgggg cgcgtcgcca tgatgggacc
     5521 gcactattcg cttgcggacg gtcaggactg ctacaccgcg gcacttgcct tcgagcgtga
     5581 cggcacgcat gtgctttgcg tcgatttcgt gccgacgacg tcgccggccg atgcacgttt
     5641 gggcgcgcgc gggagcaaac gctgaatggg aatggctgtt ggctagtgtg cccatgctcc
     5701 ggcactatcg cgaaacttta aggcgaccct aagcgcgttg ccgcgtccac gcacgttccc
     5761 agcggcgctt ttgcgccgtt tccgagattt ggcgcctcgg cccctccgtt aatcctctac
     5821 tcgcccgcgg ccggatttga tctcaccgcg cttggccttg ctgtcgaggc gccgccgatt
     5881 cgatgcgcgc gtcggccggg tcgcgacacg cggtttgcgc gtcacgctga cgctttcgat
     5941 cagttcatcc agccgcgcca gcgccgccgc gcggttcatc tcctgagtgc gatgctcctg
     6001 cgccttgatg atcaccaccc cgtctcgcgt gagccgatga tcggacagcg cgagcaggcg
     6061 catcttcagc acgtccggta acgacgaagc ctgcacgtcg aaacgcagat ggatggcact
     6121 cgagacctta ttgacgttct gaccgcccgc tccttgcgcg cgcactgcgg tcagttcgat
     6181 ttcattcggc gggatggaat aacgggatgt catgactgga ttggggctag gagacgcaag
     6241 tgtacacagg gcgcagtcgc ttgcagacta cggactgccg ggcgcggcca ccgatgcgaa
     6301 aaccatttgc atcggcgcaa ccggttcacc gatactgccg ttttcgctta cggcgtaaat
     6361 tcatatgaag ctccgtccgg gttttgtgct ggaactggcc gtcaactttc tgctgccgtg
     6421 gcttgcttac cgcctcgcgc tgccgcacct gggcgagacc ggtgcgctga tggcgtccgc
     6481 cgtgccgccg atcgtctgga gcctgatcga gttgctgcgc tttcgacgcg tcgacgcgct
     6541 cagcgtaatg gtcgtggcgg gcatcgtgct gtcggtcgcg gcgatggcgc tgggcggcag
     6601 tccccgcatg ctgttactgc gagaatcgct ggtgtccggc gcggtgggcg tggtgtttct
     6661 gctgtcgctg ccgatgcgcc gtccgctgat cttctatctc gcgcgcgcca ccgtcgcccg
     6721 tgaaatggag ggcggtgccg agcgcttcga agcgctgtgg cgcgagcggc ctgccctcgt
     6781 cggtgcgatg cggctcatga cgctggtatg gggcgtcggc ctgaccggcg agacggcgct
     6841 gcgtgcatgg atggcgctga cctggccgat cgagcgcttc ctcgtggtgt cgccattcat
     6901 cggctatggc atttacggcg ccctgacgct gtggacgctg tggtatcgaa agaccatgcg
     6961 cagccgggtc gaggcgcacg tgcagccagg cgggatcgcg ggctgagctc ggctggacgt
     7021 ccggaaacgg tcactcgatt gttctacctg ttctgcctga tccgccaagc cgccggagcg
     7081 ccagagcgcc ttaagcaggc aggccacgag cgaatatgcc agtccgccca caccacccac
     7141 taagcgacgc gccacgcctc gccaatccgc gcggccaacc ccgaatcacg ctgcgtcggt
     7201 gtctcaatgg cctgcgccag caccgcgcgc gcgccgatca cttccacccg ctcacgcgcg
     7261 cgcgtaattg ccgtgtacac aagttcgcgc gacagcacgc ggctgaacac cgacggcaac
     7321 atcagcaccg catgctgaaa ttccgagcct tgcgacttat gaaccgtgag cgcgaatgcc
     7381 gtgtcgtgtg gcggcagcgc cgctggcgat acggcacgca aaccgccgcc gccagtacgg
     7441 aaatacaccc gcaacgcgcc gcccgcgccc ggcagcgcga tgccgatgtc gccgttgaac
     7501 agaccgagcg cgtaatcgtt gcgcgtgacc atgacggggc gtccggcgaa ccattgcgcg
     7561 cctacagcca gcgtcacgcc cgccgagcgc cgcacctgcg tggccatcgc ggcattcacc
     7621 tgatcgacgc cgcgcggccc cgaacgggtc gcgcacagaa ttcgaaaacg gttcagcgca
     7681 tcgaaaagcg gcgctgggtc tggtgtatcg gcggctagcg cttcgacaag cgcatccgcg
     7741 tacggtgcaa aacccgcggc aagccgcgcg accgtgcgat cggcaagcgt cgcatgcgtg
     7801 tcttcgtgca gcgccgctgc gcagggctcg ccagggtcga tgcgcaagac gtccagcgcc
     7861 gcggcggccg cgcccttgcg aatcgcgagc gacaagcggc caatcggcga ttcgaggccg
     7921 aatcgatagt tacgctcgag ccagacgacg cagtcggcga gcgggttttg aaccggtgaa
     7981 ggtgaaggcc gtggcgctcc ggcagtgggc accgtaccac tcgcgaacag atcgtcccct
     8041 ggcagtccat cgaagacgcc gcccgcatcg gcatctacga agaagtcgcc ggggcgggta
     8101 tcgaaatcac ccggggcatg tgtatatccg aaacgatcgt caggcccttc gtcgaagccg
     8161 ttcgacgcgt ccggcaatgc ctgcgacaac cgcgccgctt caatgccaag cgccgcggcg
     8221 atccgctgca gcccgttcgg cgtaaaggca ggccgcgcgc tgagttcggc gaacaccgca
     8281 ccggcctcga cggcggcaag ctggtccttg tcaccgagca tcacgagacg cgtctgcggc
     8341 gcaatcgcat cgagaaggtg cgccgccatc gcaacgtcga tcatcgacgc ctcgtcgatc
     8401 acgacgacgt cataaggcag cggattgtcc cgatgatgcc ggaagcggcc gcccggcccc
     8461 gaaccgagca accgatgcaa ggtgtacgac gtccgcggca agcgcgcggc gagttcgggc
     8521 ggcaaatcgc cagcacgggc gagcaaggct tcctgcatcc gttgcgccgc tttgccggtg
     8581 ggcgccgcca atgcgatccg cagatcgggc cgcgcatcca gcaaacaggc gagcacaccg
     8641 accaccgtcg tcgtcttgcc ggtacccggg ccgccgctga cgatggtcaa gcgcccggac
     8701 agcgccatga ctgccgcgac acgttgccag tcgacctcgt cgtcctgcgg cggtccgaaa
     8761 taacgcagca gacgcgcgtg tagtgtttga gcggtagtgt ccgcgtttgc gtttgcatct
     8821 gctcctgctc ctgctcctgc tcctgctcct gctcctgctc ctgcatctgc tcctgcatct
     8881 gctcctgcat ctgcacccgc tcctgcatcc gcactcgcac ccgctccaac atccgctact
     8941 gcacccgccg ctgcgcctgc agccacatct gcacccacac gagaagcggc gctgccgttg
     9001 gcattgccac cctcgccagc atctgcctgt gtctctgcag atccacccgc gccagcccac
     9061 ccgcccgcat gcgcaaccag cgagcgcgca agacggcgct catagtcgta gtagcgcgcg
     9121 agatacagtc gcccttgtcc atcgatcacc aacggccgca acgccgccgc gcccggcgaa
     9181 ccgtcgctag ccatgccgct tgcgaacagc gccacgcgca cttccgcgct cgatgcatcg
     9241 aagcgtcgcg ccagcacagc aagcggcaag cacacatggc cttcggcagt ggcgcggctc
     9301 gcggcgaagg cggcccgcgc cgcccacttc accgcctcgc ccgacgcacc gccacgccgc
     9361 gccagcgtgc cgatacgacg cgcgaaccct tcggccagcg cggtactgaa atcggccggc
     9421 gcgggtaaat tcacgccgat gtcgtcgagc tccggcggcg cggaagagat ccgctcgaac
     9481 gtgctcatgc gacacctccg atcatcgccg cgtcaagcag cgccaccagt tcgaatgcgg
     9541 gccgccgcat gtgcacgccc gccggtccat cggcatcgcg ccactgcgga cgaacgccgc
     9601 gcacgaacag atagagatag ccgccgatgt gcgtctcata cgcgtaatcg cgcacccgcg
     9661 ttttcagata gcgatgcaac gccaccgtat agagcaacgc ctgcaggtga tacgcgtggc
     9721 tcgccatggc cgattcgagc ggcgccgcgg cgtagtcggc ggcggtgtcg cccagatggt
     9781 tcgacttcca gtcgacgatc cagaaacggc cgtcgtgctc gacgatcatg tcgataaatc
     9841 ctttgacgaa accacgcaac acgccgggtt ccagcgcgac gtcgggatag ccgtgttcga
     9901 tcagcagctc gcgcaacgcc ggaaaatcga gcgaaggcgc ggcaaacaga aactcaagtt
     9961 cgttcaggcg ccgccgcgga ttcagcctgg cgagcgccat gccgggcacg agctcggtcg
    10021 tcacgacgtc cgcgatcagg ttatgcatca tggcggccaa acgcgccgcg agttcgggca
    10081 cagccggcgc cgggcgctcg cgcaacgcgc cccggatggc atctggccac gtatgcggag
    10141 cggtgaaatc cgcgagttcg aacatgcgat gcagacagtc gcccgccgcc gcgccacggg
    10201 ggaacgccag gatatcgtcc tcggcgtagg acggcgcttg cggcaccgcc aaaggcgcgg
    10261 gcgcgagcgc atcggcgatc tcgtcgtgat cggggcgcac gtcttccacc gccgtatgcg
    10321 cctcctcggc tttgctgccg gcggcgatca ggccgctgaa gctcgccatg cgccactgat
    10381 cgcgcaatgg ccgccgactg gcgcgcgcgt gcatccgtgt gccgctatcc tggaggcttt
    10441 ccagcggtac gcgacgctcc actttcggca acgcctgcaa cgtgatcggg ccgcccgcca
    10501 gcgcacgcca tcgctcggac aacgcggctt cgtcgggtgg atcggtgagc cagtcaccga
    10561 agctatgacc gccgccgccc accagccagt tcagcacgct gcggcgcgac tccctggtgg
    10621 agcgcgacga aagataagta ccggcaacca gatagcagcg atacaccgcg cgcgtcagcg
    10681 ccacgtagac aagccgtgcc cgttccgccg cctgttcgcg caccgcgtaa cgtgacgcgc
    10741 gttcggcttc gtcgtcatcg caaccgtagt gcagcacggc ctcacccgtt tcgtcgtgat
    10801 attcgcgcgc atcgggcaag ccggaggacg gcggctcgcg cagcgcgccg tcgttgagaa
    10861 aaggacagaa cacaaccgcg tattccagcc ccttcgattt gtggacggtg acgatctgca
    10921 cgaggttgcg atccgactcg agccgcaact gcgcgtcttc gccaccgcct tgcgtgcgtt
    10981 gcgcggcgag ccagcgcaac gtcggcgcga tgccgggttg cgtggcggca cgcgcctgca
    11041 cgagttcggc tagatgattc acgttcgtca gacgacgctc gccgtccggg ccggccacca
    11101 gacgctgtgc gacgcgcagc tcgcgcatca gcgtgcgcca catcaaagcg aagccgcgtt
    11161 cgtgccagag cgtgcgatag cgtgaaaaac gttcgaccca acccatcgcg tcggcggcgc
    11221 gggcgggatc gtcggccggc gccggcgcat cggcgacttg ctcgaggcgc cacagcgcgg
    11281 cggcgtcgag gccgagccag tctgtcgcga gcgcggcgcg caaacgacgc agatcgcccg
    11341 gcgtatcgac cgcggccagc acgcgttcga tctgctcggc gtcgagcgtc gcgaacaccg
    11401 aggcctgcgc cagttccacg ctgccgacgc cccacaacgc cagcacacgc ttgatcaggc
    11461 tgccctgctt gtgcgtctgc acgagcaccg cgatattgcc cggcgcgagc ggcgcgtcgc
    11521 cgatcgtcac cgaccctccg cgcgcgccgc gcaggagccg cacgatctcc gcggcgcagg
    11581 cttcgctcgc cgcgcgctgt gcttcgcgtt tggtgaacgc ggcgtcaccc tggggcagcg
    11641 tccagacgcg gaaatcgccc gcgttcgctt caggctcgga gaaaggcgga cgacgccgct
    11701 cgccagcccg caccggctga tagtcgagac cgtcgagcac gaacgcctgg ggattggctt
    11761 cgaacagccg gttgcaagcc tcgacgatcg gc
//
LOCUS       DATASH010000622         2577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6035270,
            whole genome shotgun sequence.
ACCESSION   DATASH010000622 DATASH010000000
VERSION     DATASH010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6035270"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1074
                     /locus_tag="VHV99_16945"
     CDS             <1..1074
                     /locus_tag="VHV99_16945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEX3637136.1"
                     /translation="LDRLHPEKPPKYSRAVLETLAIIGYRQPVTRGDIEEIRGVTVNT
                     QVVKQLEDRGWIEVIGHRDVPGRPALYATTKQFLDDLGLSALDELPALDNPSAQLEAS
                     LLAQHAIDFPGDDAAAIGAALVQGLESKGEPALLPDASGADASPSSAILSDADVAKIT
                     PVTAESEPNGEHGAVVHAGGPDAPDTPDTPDAPDTPDALPSATTNEATQPLHSQEPAA
                     AEAVHVEGEPFLPSQAGADKPVNEENADAQTNAPVQFKPQQAVSLTGSEPLEAVPNID
                     RDPVKATPGDAVNRREVAPDAGVLTSDAARHALPHAADESDGADKADTPDASKLFDAS
                     NAPDESNETDEFNAPDDQTARRA"
     gene            1175..>2577
                     /locus_tag="VHV99_16950"
     CDS             1175..>2577
                     /locus_tag="VHV99_16950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX3637137.1"
                     /translation="MTHSHDSDSPESERPVRADDAHGQADAGERTRNDEPADGEERPR
                     RGLRRGPRSLIARRRAVAKTKGADAASSMAPAGVIAETPPDGVVAAQVRMPRKDAGSR
                     GPRNGAGSAEGARTGSVAKPRVAREGREGSTDGARDGQPPREGGRRPARQAQSAQPGQ
                     PQKRGRRDDAATPPLLAAATGTEDDVFAYVTSPAFDADNTSGDLRAPMLRRGRPAPQK
                     RVLEADDDTPKLHKVLAEAGMGSRRDMEELIVAGRVSVNGEPAHIGQRIMPTDQVRIN
                     GKPVKRKLASKPPRILLYHKPTGEIVSHADPEGRPSVFDNLPPMKTAKWLAVGRLDFN
                     TEGLLLLTTSGDLANRFMHPRYSVEREYAVRVVGELSEGMRQKLLHGVELEDGPANFL
                     RIRDGGGEGTNHWYHVALAEGRNREVRRMFEAAGLMVSRLIRTRHGPIALPKGLKRGR
                     WEELEDNEVRGLLASVGL"
BASE COUNT          527 a          810 c          838 g          402 t
ORIGIN      
        1 ctcgatcggc tacacccgga aaagccgccc aaatattcgc gcgccgtact cgaaacgctc
       61 gcgatcattg gttaccggca gcctgtgacg cgtggcgaca tcgaagaaat tcgcggtgtc
      121 acggtgaaca cgcaggtggt aaagcaactg gaagaccgcg gctggatcga ggtgatcggt
      181 catcgcgacg tgccgggccg gccagcgctc tatgccacta ccaagcagtt cctggatgac
      241 ctgggcttga gcgcactgga tgaactgccc gcactcgaca acccgtcggc gcagctggaa
      301 gcgtcgctgc tcgcgcagca tgcgatcgat ttccccggcg acgacgctgc tgcaatcggc
      361 gccgcgctgg tgcaagggct ggaatcgaag ggcgaacctg ctttgttgcc ggacgcatcg
      421 ggcgcggacg cgtcgccgtc atctgcgatt ttgtccgatg ccgacgtggc caaaatcacg
      481 ccggttaccg ctgaaagtga gccgaatggc gagcatggag cggttgttca cgctggcggg
      541 ccagacgcac cggatacgcc agatacacca gacgcaccgg atacgccgga tgctctgcca
      601 agcgcgacca cgaatgaagc gacgcagccg ctgcattcgc aggaacccgc ggcagccgaa
      661 gcagtccacg ttgaaggtga accgtttttg ccttcacaag ccggcgctga caagcctgtg
      721 aacgaggaga acgccgatgc gcaaacgaac gcaccggtgc aattcaaacc gcagcaagcg
      781 gtgtccctga ccggctcaga gccgctcgaa gcggttccga atatcgatcg ggatccagta
      841 aaagcaacgc ccggcgacgc ggtcaaccgt cgcgaggtgg cgccggatgc cggcgtattg
      901 acaagcgacg ctgcgcgaca cgcattaccg cacgccgcag atgaatccga tggcgctgac
      961 aaagcggata caccggatgc atcaaagcta ttcgacgcat cgaatgcgcc ggacgaatca
     1021 aacgaaaccg acgaattcaa cgcaccggac gaccagactg cgcgccgcgc ctgaggcctg
     1081 tttgcccgaa ccttcattcg gctgctaatc accatcggcc ggattttttg acgcgcctgc
     1141 gacgggcgcg cgacccgaca caattgaggt tgttttgaca cattcccacg acagcgattc
     1201 gcccgaatcc gagcgccccg ttcgtgccga tgacgcacat ggccaggccg acgcaggcga
     1261 acgtacacgc aacgacgaac ccgctgacgg cgaagaacgc ccgcgtcgcg gcttgcggcg
     1321 cgggcctcgc agcctgattg cccgccgtcg cgccgtcgcc aagacgaagg gcgccgacgc
     1381 tgcgtcgtca atggcgccgg ctggcgtaat cgctgagacg cctccggatg gcgtggtcgc
     1441 ggcgcaggtg cgcatgccgc gcaaggatgc aggttcacgt ggaccgcgca acggcgcggg
     1501 tagcgcagag ggtgcacgca ccggttcggt ggccaagccc cgcgttgcac gtgaagggcg
     1561 cgaaggttcg actgatggtg ctcgcgatgg ccagccgccg cgtgaaggcg gccgtcgtcc
     1621 ggcgcggcag gcgcaatcgg ctcaacccgg acaacctcaa aagcgtggtc gccgcgatga
     1681 cgcggcaaca ccgccactgt tggcagccgc aaccggtacc gaggacgatg ttttcgcgta
     1741 tgtgacatcg ccggcattcg atgccgacaa cacgtcgggc gacctgcgcg ctcccatgtt
     1801 gcgccgcggc cggcccgccc cgcaaaaacg cgtgctcgaa gcggacgacg acacgccgaa
     1861 gctgcacaag gtgctcgccg aagcaggcat gggctcgcgt cgcgacatgg aagagttgat
     1921 cgtagccggc cgggtatcgg tgaatggtga gccggcgcat atcggccagc gcatcatgcc
     1981 gaccgatcag gtgcgcatca acggcaagcc ggtcaagcgc aagctcgcga gcaaaccgcc
     2041 gcgcatcctg ctgtatcaca aaccgacggg cgagattgtc agtcacgccg atccggaagg
     2101 ccgtccttca gtcttcgaca acctcccgcc gatgaaaact gccaaatggc ttgcggttgg
     2161 acggctcgac ttcaacaccg aagggttgtt gctgctgact acatcgggag acctggccaa
     2221 ccggttcatg catccgcgtt acagcgtcga gcgtgaatac gcggtgcggg tggtgggtga
     2281 gttgtcggaa ggcatgcgtc agaagctgct gcacggcgtg gaactggaag acggcccggc
     2341 gaacttcctt cgtatccgcg atggcggcgg cgaaggcacg aatcattggt atcacgtggc
     2401 gctggctgaa ggccggaacc gcgaagtgcg ccgcatgttc gaagccgccg gcctgatggt
     2461 gagccggctg attcgtaccc ggcacggccc gatcgcgttg cccaaggggc tcaagcgcgg
     2521 tcgttgggaa gaactcgaag ataacgaagt gcgtggtctc ctggcatcgg tcggact
//
LOCUS       DATASH010000623         5049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4653263,
            whole genome shotgun sequence.
ACCESSION   DATASH010000623 DATASH010000000
VERSION     DATASH010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5049
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4653263"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1037)
                     /locus_tag="VHV99_16955"
     CDS             complement(<1..1037)
                     /locus_tag="VHV99_16955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014973234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3637138.1"
                     /translation="MKTEAQLTADAECYDAPGTGKGPFAWYRQSSAGEKRAFWSCKIG
                     YVLDAMDTQFLSFVIPTLIATWGITRGDAGLIGTVTLLSSALGGWAAGVLSDRIGRVK
                     TLQITILWFAVFTLACALAQNFSQLLWARGLMGLGFGGEWTAGAVLIGEVIRPRDRGK
                     AVGLVQAGWAIGWGVSTLLFMAVFSWIPADYAWRVLFAIGIAPAPFVIWIRRFVEEPE
                     IHRQQKEAQAAAHTRPSLLDIFRGDIVWTTLRASILATGVQGGYYAVTTWLPTYLKTE
                     RHLSVIGTGSYLGVVIVGSYCGYLAGAYVSDTIGRKRAFIAFALASCAIALIYTQLPL
                     DNLTMLVLGFPL"
     gene            complement(1116..1895)
                     /locus_tag="VHV99_16960"
     CDS             complement(1116..1895)
                     /locus_tag="VHV99_16960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006052386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydro-lyase"
                     /protein_id="HEX3637139.1"
                     /translation="MSYTPSEFRQQIRSRRLSGPTAGYCGDYAQANLAILPSQYADDF
                     LRFCTLNPKACPLLGIGEPGDWRVPSLGADLDIRNDVPAFYVYRHGERAEEVRSLDEL
                     WRDDLVVFAIGCSFSFEEMLRREGIPLRHIEQKVNVPMYRTRERNVSTGVFGGNRVVS
                     MRPMKAADAIRAIQITSRFPAVHGAPVHIGDPSLIGIRDITRPDFGDAVEVRSDELPV
                     FWACGVTPQAAIESARLPFAIAHKPGHMLVTDIPNTTLAVL"
     gene            2071..3009
                     /locus_tag="VHV99_16965"
     CDS             2071..3009
                     /locus_tag="VHV99_16965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014973235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3637140.1"
                     /translation="MNTRFVETFLTLARLGSFRATAAAMHATPAAISLRIKTLEAELG
                     VELIERDAAEFQLTAHGERLLAHARTVVQATRSLQLAAQDETQVTKRLRLGVIETVVH
                     SWLPDYIRMLNAEYNRIVVDLSVDSSAVLGPRLRAGELDLVIQVEETGEQEASIVSTL
                     LASYPVRWIARDDLIPASRAKHVQVVLQKPVLTFGRGTAPQVAVEAIVETLAKRAGVP
                     LADTQVTCMPSVAVIVKLLRDGYGIAAVPALFVEPFLSSGELGQLQVRPLPPPIDVAM
                     YYRDDADVGVLAASRVARSACDQYAKARGRQLIERR"
     gene            3135..3383
                     /locus_tag="VHV99_16970"
     CDS             3135..3383
                     /locus_tag="VHV99_16970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006053174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX3637141.1"
                     /translation="MKTTIRRMGNSHGVLIPKPILTQLGLEDEVDMQVEGNALVIRRP
                     QKKARTGWADASRSLAASKDDALVMGEFPNADDAGLEW"
     gene            3377..3697
                     /locus_tag="VHV99_16975"
     CDS             3377..3697
                     /locus_tag="VHV99_16975"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011879712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEX3637142.1"
                     /translation="MVARGEVWLVALDPTVGSEIQKTRPCVVVSPGELHDHLRTVIVA
                     PMTTAGRAAPFRVPLTFLRKKGLILLDQIRTVDKTRLVKRAGSVSDTALSNALSTLQE
                     IFVE"
     gene            complement(3801..4724)
                     /gene="mdcH"
                     /locus_tag="VHV99_16980"
     CDS             complement(3801..4724)
                     /gene="mdcH"
                     /locus_tag="VHV99_16980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit epsilon"
                     /protein_id="HEX3637143.1"
                     /translation="MLALLFPGQGAQSDGFLHSLPQHQAVRETLDEASQVLGIDVLTL
                     DTPDSLGSTVAVQIGLTVAGVAITRALAEEQLTPGISAGLSVGAYAAAVCCGAIRFED
                     ALKMVRKRAELMETAYPSGYGLAAVSGLTEHQLETLAEQHAEANNQRVYIGNVNAPRQ
                     IVMAGANTALDAFMERALAAGARKATRLAVSVPSHCELLAHATDELLAYAQDVPFHVP
                     RSTYIGNRGGRPLYTAQAIRDDLATNMRYTVRWFDALSVMQEMGARVLIEAPPGQVLT
                     DITRDNFPDTIALAASTFSFGKLVATARRRL"
     gene            complement(4724..>5049)
                     /locus_tag="VHV99_16985"
     CDS             complement(4724..>5049)
                     /locus_tag="VHV99_16985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HEX3637144.1"
                     /translation="LVEFVWGPTGSAGFELATQAPTVTLSSDLDLLIRAPEKRSHANA
                     VQLLDYLQTIAQRAGIRMDAQLETPAGGVALAEWAADKARVMVRHARGPQLIADPWAP
                     VHGDA"
BASE COUNT          967 a         1523 c         1626 g          933 t
ORIGIN      
        1 agcgggaaac cgagcacgag catggtgagg ttatcgagcg gcagttgcgt gtagatcaac
       61 gcgatcgcac acgaggccag cgcgaacgcg atgaaggcgc gcttgcggcc gattgtgtcg
      121 ctgacgtaag cgcccgcgag atacccgcag tacgaaccga cgatcaccac gcccagatag
      181 ctgccggtgc cgatcacgct cagatgccgc tcggttttca gatatgtcgg cagccatgtg
      241 gttaccgcgt agtagccgcc ttgcacgccg gtcgcgagaa tggacgcgcg cagcgtcgtc
      301 cagacgatat cgccgcggaa gatgtcgagc agcgaagggc gcgtgtgtgc cgcggcttgc
      361 gcttctttct gctgccgatg tatctccggc tcttcgacga agcggcggat ccagatcacg
      421 aacggcgccg gcgcaatacc gattgcgaac agcacgcgcc acgcgtagtc ggccggtatc
      481 catgaaaaga cggccatgaa caacagcgtc gacacgcccc agccgatcgc ccagcccgcc
      541 tgcaccagac cgaccgcctt gccgcggtca cgcggacgga tcacttcgcc gatcagcacc
      601 gcgccggccg tccactcgcc gccgaagccg agtcccatca ggccgcgcgc ccacagcaac
      661 tgcgaaaagt tctgcgccag cgcgcaagcc agcgtgaaca ccgcgaacca gaggatcgtg
      721 atctgcaagg tcttcacccg gccgatgcga tcggacagca caccggccgc ccagccgccg
      781 agtgccgaac tcagcagcgt gaccgtgccg atcagaccgg cgtcgccgcg tgtgatgccc
      841 catgtcgcaa tcagcgtcgg aatcacgaag ctcaggaatt gcgtgtccat tgcgtcgagc
      901 acgtagccga tcttgcagct ccagaacgca cgcttctcgc cggcggaact ctgccgatac
      961 cacgcgaacg gtcctttccc tgtgccgggt gcgtcgtagc attcggcgtc cgcggtcaat
     1021 tgcgcttcgg ttttcatgta tgggctccgg tcgtagtaag cggccgcgtc ttgccgcgcg
     1081 gctcgtggtt gggatagtgg atcaagacgc cgcggctaga ggaccgccag cgtggtgttg
     1141 ggaatgtcgg tgacgagcat gtgtcccggt ttgtgcgcga tcgcaaacgg caaacgcgcg
     1201 ctttctattg cggcctgcgg tgtgacgcca cacgcccaga acacgggcaa ctcgtcgctg
     1261 cgcacttcta ccgcgtcgcc gaaatcgggc cgcgtgatat cgcgaatgcc gatcagcgaa
     1321 gggtccccga tgtgaaccgg cgcgccatgt accgcgggaa aacggctggt gatctggatg
     1381 gcgcggatcg cgtcggcggc tttcatcggc cgcatcgaga ccacgcggtt gccgccgaac
     1441 acgccggtgc tcacgttgcg ttcgcgcgtc cgatacatcg gcacgttgac cttctgttcg
     1501 atatggcgca gtggaatgcc ttcacgccgc aacatctcct cgaacgaaaa cgagcaaccg
     1561 atcgcgaaca ccaccaggtc gtcgcgccac agttcgtcga gactgcgcac ctcctcggca
     1621 cgttcgccgt ggcggtacac gtaaaacgcg gggacgtcat tgcgtatgtc gagatcggca
     1681 ccgagcgacg ggacacgcca atctccgggt tcgccgatgc cgagcaacgg gcaagccttc
     1741 ggattgagcg tgcagaaacg caggaagtcg tcagcgtatt ggctgggcag gatggcgagg
     1801 ttggcttgcg catagtcgcc gcagtagccc gccgtcggcc cggacaggcg gcggctgcga
     1861 atctgctgac gaaactcgga tggcgtgtag gacatgaacg gttcgactcc agctaggtcc
     1921 gggtgactga tgaaagccag attagtaacg tcaaaaatat ccatccagcg agttattctt
     1981 gcgtttcgtt aaattaaact taacggcgcc gtggtgaatt tgcggtgatg atcgaaggcc
     2041 ttcgggaatg ctgttgggag agctgatgca atgaataccc gctttgtcga aacgtttctg
     2101 acgctcgccc gtctaggcag ttttcgagcg accgctgccg ccatgcacgc gacgcccgcg
     2161 gcgatttcgc tgcgcatcaa aacgctcgaa gccgagttgg gcgtcgagtt gatcgagcgc
     2221 gacgccgcgg agtttcaact tacggcgcat ggcgaacggc tgctcgcgca tgcgcgaacg
     2281 gtggtgcagg ccacgcgctc gctgcaactg gccgcgcagg acgaaaccca ggtgaccaag
     2341 cggctgcggc tcggcgtcat cgaaacggtg gtgcatagct ggctgccgga ctacatccgc
     2401 atgctgaacg ccgagtacaa ccgtatcgtc gtagacctga gcgtcgactc gagcgccgtg
     2461 ttagggcccc ggctgcgcgc gggcgagctg gatctcgtca ttcaggtgga ggaaaccggt
     2521 gagcaggaag cgtcgattgt ctcgaccttg ctggcgagtt atccggtgcg ctggatcgcg
     2581 cgcgacgacc tgattccggc gagccgcgcg aaacacgtgc aggtggtatt gcagaaaccc
     2641 gtgctgacgt tcggccgtgg taccgcgcca caagtcgccg tggaggcgat cgtggagacg
     2701 ctcgcgaagc gcgccggtgt gccgcttgcg gacactcagg tgacctgcat gccgtcggtg
     2761 gcggtgatcg tgaagctgtt gcgcgatggc tatgggattg cggcggtgcc ggcgctgttc
     2821 gtcgagcctt tcctgagcag cggtgagctg gggcaattgc aggtgcgccc gctgccgccg
     2881 ccgatcgacg ttgcgatgta ttaccgcgac gacgccgacg tgggcgtgct ggcggcctca
     2941 cgcgtcgcgc gtagcgcgtg tgatcagtat gcgaaggcaa gggggcggca gttgatcgag
     3001 cggcggtgag gcgagcgatc aacaaggcac ggaaaacgcg tcattcgtct tatgccattt
     3061 gccgtgctac actgtaataa cattgtgttt acttttaaat gcctaccctt taggattcca
     3121 gtctaggtgc tgccatgaaa acgaccatcc gcagaatggg taactcccat ggtgtactga
     3181 ttccgaaacc tatcttgacc caacttgggc tggaggacga agtggatatg caagtagaag
     3241 ggaacgccct cgtgatacgg cgtccgcaaa aaaaagcgcg tacgggctgg gccgacgcga
     3301 gccgttcgtt ggcggcgtcg aaagacgacg cactggtgat gggcgagttt cccaatgccg
     3361 atgacgcggg gctcgagtgg tagcgcgcgg cgaagtctgg cttgtcgcgc tggatccaac
     3421 cgtcggcagc gagatacaga aaacgcgtcc atgcgtagtt gtttcgccgg gcgaattgca
     3481 cgatcacctg cgcacggtga tcgtcgcgcc aatgaccact gctggccgtg cggcgccgtt
     3541 tcgtgtcccg ctcactttct tgcgcaagaa aggtttgatc ctgctcgacc agattcgcac
     3601 ggtagacaag acgcggcttg tcaagcgtgc aggctccgtc tcggatacgg cgctatcgaa
     3661 tgcgttgtcg acgttgcagg aaattttcgt cgagtgaagg actgcaggtg gcgtaggccg
     3721 agtggcggcg tagtggggct taatccgggt gaataacccg ggtgctcatt ccaggcgttc
     3781 aatctacgca ttcaagcggc tcagaggcgc cgtcgggcag tcgccacgag tttgccgaac
     3841 gagaaggtac tcgcagcaag cgcgatggtg tccgggaaat tatcacgtgt gatgtccgtc
     3901 aacacctggc ccggcggtgc ttcgatcagc acgcgggcgc ccatttcctg catgacgctg
     3961 agcgcgtcga accagcgcac ggtgtagcgc atattggtcg cgagatcgtc gcgaatcgcc
     4021 tgagccgtgt acaaaggccg accgccgcga ttgccgatat aggtgcttcg aggcacatga
     4081 aacggcacat cctgtgcgta cgcaagcagc tcgtctgtgg catgggcgag cagttcgcaa
     4141 tgcgacggca cgctcacggc gagacgggtg gccttgcgag cgcccgccgc tagcgcgcgc
     4201 tccatgaatg cgtccagcgc ggtgttcgca cccgccataa cgatctgacg tggcgcattc
     4261 acattgccga tatacacacg ttgattgttc gcctccgcgt gctgctcggc gagtgtttcc
     4321 agttggtgtt cggtcaaacc cgatacagcc gcaagaccgt agccggacgg gtatgccgtt
     4381 tccatcaact cggcgcgctt gcgaaccatc ttcaacgcgt cctcaaagcg aatcgcgccg
     4441 caacagaccg ccgctgcata cgcgcccacc gacaaacccg cgctaatccc cggcgtgagc
     4501 tgttcctctg ccagtgcgcg tgtgatcgcc acgcccgcga ccgtcaggcc gatctgaacg
     4561 gctacggtcg agcctagcga atctggcgtg tcgagtgtga gtacgtcgat gccgagcacc
     4621 tgagacgctt cgtcgagtgt ttcgcgcaca gcctggtgct ggggcagact gtgcaggaat
     4681 ccgtccgact gggcgccctg gccgggaaac agaagggcaa gcatcaggca tcgccgtgaa
     4741 caggtgccca cggatcggca atgagctgcg ggccgcgagc gtggcggacc atcacgcgtg
     4801 ctttgtccgc ggcccactcg gccagcgcaa cgccgcccgc gggtgtttcc agttgcgcat
     4861 ccatgcgaat gccggcgcgt tgcgcgattg tttgcagata atccagcaat tgaacggcgt
     4921 tcgcatgcga gcgcttttcc ggcgcccgga tcagcagatc gaggtcactc gagagcgtga
     4981 cagtgggtgc ctgagtggcc agttcaaagc cagcgctgcc ggtcggcccc cacacgaatt
     5041 caaccaatg
//
LOCUS       DATASH010000624         4132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5838662,
            whole genome shotgun sequence.
ACCESSION   DATASH010000624 DATASH010000000
VERSION     DATASH010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5838662"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..914
                     /gene="dapA"
                     /locus_tag="VHV99_16990"
     CDS             39..914
                     /gene="dapA"
                     /locus_tag="VHV99_16990"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012400822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEX3637145.1"
                     /translation="MRGSIPAIITPMLEDGSLDLPAFRKLIDWHIAEGTNALVVVGTS
                     GESATLSVEEHVLMVKTAVEHTAGRIPVIAGSGGNSTTEAIELTQQAKEVGAHASLQV
                     VPYYNKPTQEGIYRHFAKIAETVDLPVILYNVPGRTVADMSNETILRCAQVPGIIGVK
                     EATGNIDRAAHLIKSAPAHFGIYSGDDPTAIALMLLGGHGNISVTANVAPRAMSDLCK
                     AALAADAKTAREIHLKLLSLHKNLFIESNPIPAKWALQQLGRVQGGIRLPLTPLDAQY
                     HEVVRGALREAGLLG"
     gene            1043..2182
                     /gene="bamC"
                     /locus_tag="VHV99_16995"
     CDS             1043..2182
                     /gene="bamC"
                     /locus_tag="VHV99_16995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HEX3637146.1"
                     /translation="MKRSALSLHATRMAALALALVTLAGCDTLNDWFASDRVNYKGAG
                     SAPPLAVPGDLTTTSTDQRYVAPPANLALGGSPQRAVTAAGNSTEGQPNAQDPLGMHI
                     ERDGDRRWLVVDGRTPEQLWPQLQEFWQENGFALKTDAPATGIMTTDWAENRANIPDD
                     WFRRTVGKVIDFAYSSGTRDSFRTLVSRGPVDTTDISITHSAMEEVLTGQDKTSSRWE
                     ERPRDPALEALFLTKLMQKFGLTEAQSKQLLTDAHPAAAPATIEKDAGTSTLDLQESF
                     DRAWLRVGLALDRTNFTVDNRDRAKGIYYVRYADSMQELKREGLFGKLFYSGNSSKKP
                     GQEFLVNVRSKGDTVTQVAVLDANGQVDNSSDAQRIVTLLHAQLN"
     gene            2190..2963
                     /locus_tag="VHV99_17000"
     CDS             2190..2963
                     /locus_tag="VHV99_17000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007580406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX3637147.1"
                     /translation="MRFASLGSGSEGNALLVEAQSGATTTRVLLDCGFSAKEVERRLT
                     RLGAGVEGLDAILITHEHSDHIGSALTLARKWSIPLYMSWGTARAVGADEADVDLQVL
                     WGDEAVAIGDLSILPYTVPHDAREPLQYVLSNGASRLGVLTDVGTSTPHISAVLSGCD
                     ALVLECNHDVRMLAGSRYPQSLKARIGGSQGHLNNEAAAEILASLDRSRLRHLVAAHL
                     SQQNNSPELAQAAMAGVLGAAPTEVVVASQDEGFDWLSL"
     gene            complement(3106..3804)
                     /locus_tag="VHV99_17005"
     CDS             complement(3106..3804)
                     /locus_tag="VHV99_17005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013698250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEX3637148.1"
                     /translation="MNDHPYSFGRNGAVSTVETRNRVLRNTYWLLALSMIPTVLGAWV
                     GVATGFSLFAATSPGMSMLAFFAIAFGFMFAIQKTKDSAVGVFVLLGFTFFMGLMLSR
                     ILSFVLGFSNGPSLIMLAFGGTGVIFASMATIATVSKRDFSGLGKWLFMGVVVLLLAS
                     VANVFLHLPALMLTVSVLAIVIFSAYMLFDVQRVVNGGETNYITATLAIYLDLYNVFV
                     NLLALLGIFGGNRN"
BASE COUNT          843 a         1271 c         1284 g          734 t
ORIGIN      
        1 tgactaacgg caatcaaagc agcactggcg gcgttcagat tcgcggcagc atccctgcca
       61 tcatcacccc gatgctcgaa gacggcagcc tcgatctgcc ggcgtttcgc aaactgatcg
      121 actggcatat cgcagagggt acgaacgcgc tggtcgtggt cggcacgagc ggcgagtcgg
      181 ccacgctgtc ggtcgaagaa cacgttctga tggtcaaaac cgcagtcgag cacacagcgg
      241 gccggattcc ggtgatcgcg ggctcgggcg gcaattccac cactgaagcc atcgagctca
      301 cgcagcaggc caaagaggtc ggcgcgcacg cctcgctgca ggtcgtgccg tactacaaca
      361 agccgaccca ggaaggcatc tatcgccact tcgcgaagat cgccgaaacc gtcgatctgc
      421 cggtgatcct gtataacgtg ccgggccgta cggtcgcgga catgagcaac gaaacgattc
      481 tgcgttgcgc gcaggtgccg ggcatcattg gtgtgaagga agcgacgggc aacatcgatc
      541 gcgccgcgca cctgatcaag tcggcgcccg cgcatttcgg catctacagc ggtgacgatc
      601 ccacggcgat cgcgctgatg ctgctcggcg gccacggcaa tatttcggtt accgcgaacg
      661 tcgcgccgcg cgcgatgagc gatctgtgca aagccgcgct cgccgcggac gccaaaaccg
      721 cccgcgaaat tcatttgaaa ctcctgtcgc tgcataagaa cctgttcatc gaatcgaacc
      781 cgattcccgc gaaatgggcg ttgcagcaac tgggtcgtgt gcagggcgga atccgcctgc
      841 cgcttacgcc gctcgacgcg caataccacg aagtggtgcg tggcgcgctg cgcgaagcgg
      901 gcttactggg ttaaacgggc tcgcccggca tcccagcgtt aaacgaaaga ctgccaccgg
      961 cactccctta accgacgtcg atccagccca cgttcccggc acacagaacc aggcaccgcg
     1021 ttccagcatc acgaaggacc tcatgaaacg ttccgcactt tccctccacg caacccgcat
     1081 ggcggcgctg gcgcttgcct tggtaacgct cgccggctgt gacacgctga acgactggtt
     1141 cgcctccgac cgcgtcaact acaagggcgc cggcagcgca ccgccgctcg ccgttccggg
     1201 cgatctgacc accacgtcga ccgatcagcg ctacgtggcg ccgcccgcta atctggcgct
     1261 gggcggctcg ccgcaacgcg cggtcaccgc agccggcaat tccaccgaag gccagccgaa
     1321 tgcgcaagac ccgctgggca tgcacatcga gcgtgacggc gatcgtcgct ggctggtcgt
     1381 cgacggccgc acgccggaac aactgtggcc gcaactgcag gagttctggc aggagaacgg
     1441 tttcgcgctg aaaaccgatg cgccggccac cggcatcatg accaccgact gggctgaaaa
     1501 ccgcgcgaat attccggacg actggttccg tcgcacggtc ggcaaggtga ttgacttcgc
     1561 ttactcgtcg ggcacccgtg acagcttccg cacgctcgtg tcgcgcggtc cggttgacac
     1621 cacggacatc tcgatcacgc atagcgcgat ggaagaagtg ctgacgggcc aggacaagac
     1681 gtcgtcgcgc tgggaagagc gtccgcgcga tccggcgctg gaagcgctgt tcctgaccaa
     1741 gctgatgcag aagttcggtc tgaccgaagc gcagtcgaag caactcctga ccgacgcgca
     1801 tccggctgcc gcgccggcca ccatcgaaaa ggacgcgggc acgtcgacac ttgacctgca
     1861 ggaatcgttc gaccgtgcat ggctgcgcgt gggtctcgcg ctcgaccgca ccaacttcac
     1921 cgtcgacaat cgcgatcgcg cgaaaggcat ctactacgtc cgttacgcgg actcgatgca
     1981 ggagctgaag agagaagggc tgttcggcaa gctgttctac agcggcaatt cgtcgaagaa
     2041 gccgggtcag gaattcctcg tcaatgtgcg ctcgaagggt gacaccgtga cgcaggtggc
     2101 ggtgctcgac gcgaacgggc aagtggataa ctcgtccgac gcgcagcgca tcgtgacgtt
     2161 gctgcacgcg caactgaact aagcgggctg tgcgcttcgc cagtctcggc agcggcagtg
     2221 aaggcaatgc cttgctggtg gaggcgcaaa gcggcgcgac caccacgcgc gtgctgcttg
     2281 attgcggctt ttcggccaag gaagtcgagc ggcgtttgac gcggctcggt gcgggcgtcg
     2341 aaggtcttga cgccattctg attacacatg aacacagcga ccacattggc agcgcactca
     2401 cgctggcacg caagtggtcg attccgttgt acatgagctg gggcaccgcc cgagcggtgg
     2461 gtgccgacga agccgacgtc gacctgcagg tattgtgggg cgacgaagcg gtggcgatcg
     2521 gcgacctcag catcctccct tacaccgttc ctcacgacgc ccgcgagccg ctgcaatatg
     2581 tgctctccaa tggtgcgagc cggcttggcg tgctgaccga cgtcggtacc tcgacgccgc
     2641 acatcagcgc ggtgctgagc ggctgcgatg cgctggtgct cgaatgtaat catgacgtgc
     2701 ggatgctggc gggtagccgc tatcctcagt cgctgaaagc gcgcatcggc ggcagtcaag
     2761 ggcatctgaa caacgaggcg gcggctgaaa tcctggcctc gctcgatcgt tcgcgcttgc
     2821 gtcatcttgt ggctgcgcat ctgagccagc agaacaactc gccggaactg gcgcaggctg
     2881 caatggcggg tgtgttgggt gccgcgccga ccgaagtggt ggtcgcttct caggacgaag
     2941 gcttcgactg gttgagcctg tgatccgcct ctgatgccat gtatcgcctg cgcagcgttt
     3001 cccgtcgacg tgccggccgc tcaataaaaa aaggcccatg gcgattgctc gccatgggct
     3061 ttttcttcaa ggcggacgtt tcgggcgccg cgccttgcgt caaacttagt tgcggttgcc
     3121 gccgaaaatg ccgagcagcg cgagcaggtt gacgaacacg ttgtacaggt ccagatagat
     3181 cgcgagggtg gcggtgatgt aattcgtctc accgccgttc acgacgcgtt ggacgtcgaa
     3241 cagcatgtag gccgagaaga tcacgatggc cagcaccgaa accgtcagca tcaacgccgg
     3301 caagtgcagg aacacgttcg cgaccgaagc cagcagcagc acgaccacgc ccatgaacag
     3361 ccacttgccg agacccgaga agtcacgctt gctgaccgtg gcgatcgtgg ccatggaggc
     3421 gaagatcaca ccagtgccac caaaggcgag catgatcagc gaagggccgt tcgaaaaacc
     3481 gagcacgaag ctcaggatgc gcgacagcat caggcccatg aagaacgtga agccgagcag
     3541 cacgaacaca ccgacggcgc tgtctttggt tttctggatg gcgaacatga agccgaacgc
     3601 gatcgcgaag aacgccagca tgctcatgcc ggggctggtg gcggcgaaca gcgagaagcc
     3661 ggttgccacg cccacccatg cgccgagcac ggtcggaatc atggatagcg ctagcagcca
     3721 gtaggtgttc cgcagtacgc ggttgcgtgt ttcgaccgtg ctgactgcgc cattgcggcc
     3781 aaagctatac ggatgatcgt tcatggtctc tccttacctc agaagcgtgt tgcgtaatgg
     3841 ttgtgccgcg ggtactgcag caggttgtgt caggtcgtac tgcgccggcc gggattttcg
     3901 cggccgatcg cagcgtgtcc atccctaaga ttggcgcgat ggctgggagt ttcaatccct
     3961 cgaaaatgtc catacggcct acgctaccag tatttggaaa ctcctgctcg gagtcttaca
     4021 gtggtgtaag ggtccaatca caatcataca cgggaacatg ctacaatagc ggattcattt
     4081 gaatctgtaa cctcttaatt ttttggagtt tttatggcga tcgaacgcac cc
//
LOCUS       DATASH010000625         3095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2477104,
            whole genome shotgun sequence.
ACCESSION   DATASH010000625 DATASH010000000
VERSION     DATASH010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2477104"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="VHV99_17010"
     CDS             <1..755
                     /locus_tag="VHV99_17010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX3637149.1"
                     /translation="PMKAFSGIEPMMFRATPTPGGYLINGTLPWVSHIGLGQYCGAIA
                     AVHRDDGSVSHEVMFQLSCDSRVELRHCPVFSGMEGTSTWGVKLTNYFVGEADLIADP
                     ARPFVARIKAAFILLQVGMALGVAQGGIDSCLEVEPVLGHVNRFLHDRPDELTAELDE
                     AVSATQRLARTAYDGSTDYLLEVLDLRTRGAELALRAAQASLLHQGARGYLMSAAPQR
                     RIRESHFVAIVTPAIKHLRWEMARLSAEELPA"
     gene            752..2179
                     /locus_tag="VHV99_17015"
     CDS             752..2179
                     /locus_tag="VHV99_17015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3637150.1"
                     /translation="MSVVIADWKKYVCRACGVIYDEELGDPDSGIAAGTRFDAIPDDW
                     ECPLCGVTKADFVLYEPVVAGAAACAPAQGASSRTPGVIVVGGGTAGWAAVEAVRALD
                     PDIPITLVSACPADRYRKPELSVSLSEKRTPERLVFETGPAAATRLGVRLVAHTYAVG
                     LSPALHQLRTTAGTFRYRQLILAQVARPVAPSGLPRELCWRVNDLSMWSSMQRRLTAG
                     RARVAVIGAGLIGCELAEDLARAGHTVTVLDVSDRPLATLLPTPASRRLLAAWTRLGI
                     HFLPGRSVSVEARRGIDACATERTSDATTASGGVADVSATRSEKVITTQHGERIAADL
                     VLCAVGLATEGRLARMAGLEFEHGIVVDCRTLQTSVPDIYALGDCISFAGEPCRFIEP
                     IVAQAEAIAHAVTGCEHAGYRHQSPVIRVKTRSTPIVVRGLPSRELEWQIVEEDDGHL
                     AMAQYRDGDIVSSLTVGSRRVELAA"
     gene            2206..>3095
                     /locus_tag="VHV99_17020"
     CDS             2206..>3095
                     /locus_tag="VHV99_17020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX3637151.1"
                     /translation="MGNRYQPFDPGSTLGGCSCGRHADEAAHRAATAGTAHDAHEGRS
                     ADGEAAWRDAIEAAAVRALFPRNSMRRAFIRAVGKSTAIAAVYSVLPIASLQQAFAET
                     KATQLEKRDLNVGFLPITCATPLIAAEPLGFYKEEGLNVTLNKVGGWALVRDRMLNRE
                     LDASHMLAPMPLAISLGVGSQPTPTRVATIQNVNGQAIVLALRHKDNRDPRNWKGFRF
                     AIPFDYSMHSFLLRYYLAEHGIDPDRDVQLRVTPPAEMIANLRAGNIDGFLGPDPFNQ
                     RAVFDEVGFIHILSKDIWAG"
BASE COUNT          526 a          995 c         1055 g          519 t
ORIGIN      
        1 atccgatgaa agcgttctca gggatcgagc cgatgatgtt ccgcgccacg cccacgccag
       61 gcggctatct gatcaacggc acgctgccgt gggtcagtca catcggcctc ggtcagtatt
      121 gcggcgccat cgccgcagtg catcgcgacg acggttcggt ctcgcacgag gtaatgttcc
      181 agctgagctg cgactcgcgc gtcgagttgc gccactgtcc ggtgttctcc ggcatggaag
      241 gcaccagcac gtggggcgtc aagctcacca actatttcgt cggcgaagcc gacctgattg
      301 ccgatccggc gcgaccattt gttgcgcgga tcaaggcggc cttcatcctg ctgcaggtcg
      361 gcatggcgct gggcgtggca caaggtggta tcgattcgtg tctcgaagtc gagccggtac
      421 tcggtcacgt gaaccgcttc ctgcacgacc ggcccgacga actgacggcc gaactcgacg
      481 aagcggtgag cgcgacgcag cgacttgcgc gcaccgccta cgacggcagc actgactatc
      541 tgctggaagt gctcgatctg cgcacacgcg gcgcggaact cgcgttgcgt gcggcgcagg
      601 cgtcgctgct gcatcagggc gcgcgcggct atttgatgag cgcagcgccg cagcggcgga
      661 ttcgcgagtc gcacttcgtg gcgatcgtga cgcctgccat caagcatctg cgttgggaaa
      721 tggcccgtct ttccgccgag gagcttccag catgagcgtc gtgattgccg actggaagaa
      781 gtacgtctgc cgcgcgtgcg gtgtgatcta tgacgaagaa ctgggggatc ccgatagcgg
      841 cattgcggcc ggcacacgct tcgacgcgat ccccgacgac tgggagtgcc cgctatgcgg
      901 ggtaacaaaa gcggatttcg tgctttacga gccggttgtg gccggtgcag cggcgtgcgc
      961 gccggcgcag ggcgcgtcgt cgcgcacccc gggggtaatc gtggtcggcg gcggcacggc
     1021 gggttgggca gccgtcgaag cagtccgggc gctggatccg gacataccga tcacgctggt
     1081 gagcgcctgc ccggcggacc gttaccggaa gccggaattg tcggtttcgc tgagcgagaa
     1141 acgcacaccg gagcggctcg tattcgagac cggcccggcc gccgcaacac gcctcggcgt
     1201 acggctggtc gcccatacct acgcggttgg gctgtcgccg gcgctgcatc agctgcgcac
     1261 gacggcgggc acgttccgtt accggcaact gattctcgcc caggtggcgc ggcccgtggc
     1321 gccgtccggc ttgccgcgcg aactgtgctg gcgcgtcaac gatctgtcga tgtggagcag
     1381 catgcagcgc cggctgacgg cggggcgggc gcgcgtggcg gtgatcggcg cgggtctgat
     1441 cggctgcgag ctggcggagg acctggcacg ggcgggacat acggtcaccg tgctggatgt
     1501 gagcgacagg cccctcgcga cgttgttgcc gacgccggcc tcgcgccgcc tgctcgccgc
     1561 ctggacgcgg ctcggtatcc actttctgcc cggccgcagc gtgagcgtcg aggcgcggcg
     1621 tgggatcgac gcgtgcgcca cagagcggac gagtgacgcg acgacggcaa gcggcggtgt
     1681 cgcggatgtc agcgccacgc gaagcgaaaa ggtgatcacg acgcagcacg gcgagcggat
     1741 tgccgccgat ctggtgctgt gcgccgtggg gctcgccacc gaagggcgcc tcgcaagaat
     1801 ggcaggactc gaattcgagc acggcatcgt agtggactgc cgcacgttgc agaccagcgt
     1861 gccggatatc tacgcgctcg gcgactgcat cagcttcgcc ggcgagccgt gccgcttcat
     1921 cgagccgatt gtcgcgcagg ccgaagcgat cgcgcacgcg gtgaccggtt gcgaacatgc
     1981 gggctatcgc caccagtcgc ccgtgatccg cgtgaagacg cgctcgacgc cgatcgtcgt
     2041 gcgcggactg ccttcgcgcg agctggagtg gcaaatcgtc gaagaagacg acggacacct
     2101 cgcgatggcg cagtatcgtg atggcgacat cgtttcgtcg ttgacagtcg gtagccggcg
     2161 cgtcgagctc gcggcctgaa ccaaccctca cgcatcggac accacatggg taaccgctat
     2221 caaccgttcg accccggcag cacgcttggc ggctgctcat gtggccgcca tgccgatgaa
     2281 gccgcgcacc gcgccgccac ggcgggcacc gcgcacgacg cgcatgaagg acgcagcgcg
     2341 gacggtgaag ccgcgtggcg agacgccatc gaagcagcgg ccgtacgcgc actctttcct
     2401 cgcaactcga tgcggcgtgc ctttatccgt gcggtcggca agagcacggc catcgccgcc
     2461 gtgtacagcg tgctgccgat cgcgtcgctc cagcaggctt tcgcagaaac gaaagccacg
     2521 cagctcgaaa agcgcgacct caacgtcggc tttctgccga tcacctgcgc cacgccgctg
     2581 atcgccgcag agccgctcgg cttttacaaa gaggaagggc tgaacgtcac gctgaacaag
     2641 gtgggcggct gggcgctggt gcgcgaccgg atgctgaatc gcgaactcga cgcatcgcac
     2701 atgctcgcgc cgatgccgct agcgatttcg cttggcgtcg gttcgcagcc cacgccgacg
     2761 cgcgtcgcga cgatccagaa cgtgaacggc caggccatcg tgctggcgct cagacacaag
     2821 gacaaccgcg acccgcgcaa ctggaaaggt ttccgcttcg cgattccgtt cgactattca
     2881 atgcacagct ttctgcttcg ctattacctc gcggagcatg gcatcgatcc ggatcgcgat
     2941 gtgcaactgc gtgtgacacc gcccgcggag atgatcgcga acctgcgcgc gggcaacatc
     3001 gacggatttc tgggacccga tccgtttaac cagcgcgcgg tattcgacga ggtcggtttc
     3061 attcacattc tgtcgaagga catctgggcg gggca
//
LOCUS       DATASH010000626        14728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5244752,
            whole genome shotgun sequence.
ACCESSION   DATASH010000626 DATASH010000000
VERSION     DATASH010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 14728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14728
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5244752"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1211)
                     /locus_tag="VHV99_17025"
     CDS             complement(<1..1211)
                     /locus_tag="VHV99_17025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016346870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEX3637152.1"
                     /translation="MSTVVFLGLILFSLYLAIRSKKGHGAQSVHDFFVASRQFGAYLV
                     FFLAAGEIYSIGTMVGFPGGIYAKGPTYGVWFLGYILLAYPVGYFIGPKIWEAGKRYN
                     AITLPDLFKGHFGSRALELIVAGSAILFLLPWGELQFTGLVAAFKGLGWNFQPLYLIL
                     ISAVLAFTYIAISGVRASAYIAILKDILMVAAIVITGLAVSWESGVTEVFHAASLHVS
                     NSMNASQLTFSMSTMLFQSLGFYVMPFAVQVFFTAASPNTIRRTQVAMPLYMLMYPFL
                     VIASYYAISQNLHLASPNEAFFAAAVRLLPGWLLGLVAAGAALSGLLVLTGICLAIGP
                     IVTRNLLPKMPESKQKASAKMVIVAYLLVSIVMTLLTPNLMLTLINTAYYGVTQFFPG
                     VMIILFSLRVNP"
     gene            complement(1230..1439)
                     /locus_tag="VHV99_17030"
     CDS             complement(1230..1439)
                     /locus_tag="VHV99_17030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3311 domain-containing protein"
                     /protein_id="HEX3637153.1"
                     /translation="MLKFLIGIGIPYIGVIGLLPWVASVDRFVLGVPFIYAWIFAWFV
                     LTSLCLQVCWRLFDRRADDAHPKTH"
     gene            1750..2217
                     /locus_tag="VHV99_17035"
     CDS             1750..2217
                     /locus_tag="VHV99_17035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011694727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX3637154.1"
                     /translation="MASNDTQPTRTPSDADALHATAEDLRVLVGKLRRRLREEAHVGD
                     FTPSQVQVLGLLEREGPATVTTLARIQGMRPQSMGETLSVLKAAGLVSGAPDPNDGRQ
                     TVLSLTPAFRKKVKASRTAREDWLFRTIQTRFSAAEQKQLAIGIDLLKRLIDL"
     gene            2234..2797
                     /locus_tag="VHV99_17040"
     CDS             2234..2797
                     /locus_tag="VHV99_17040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX3637155.1"
                     /translation="MALTTLDAKTALLVIDLQRGIVALPTAHPTAEVVTRSASLADAF
                     RRHGLPVVLVNVAGGAPGRAEQSRHTGDFPAGFADLVPEVNQQPSDHLVTKRTWGAFT
                     QTDLEAYLRKQGVTQVVLVGVATSIGVESTARFAHELGFNVTFAVDAMTDMNLDAHTN
                     SIARIFPRLGESGTTQDVLDLLEKSRA"
     gene            2850..4190
                     /locus_tag="VHV99_17045"
     CDS             2850..4190
                     /locus_tag="VHV99_17045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014973081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3637156.1"
                     /translation="MSGTFRSLRTFNYRVWASGAIVSNIGTWMQRTAQDWLVLTELTH
                     HNATSVGIVMSLQFGPQMLLLPLTGYAADHFDRRKLLFATQAAMGTLALCLGLLTVTG
                     LVRLWQVYMFAGLLGCVTAFDSPARQTFVSDLVGEADLSNAVALNSTSFNAARMIGPA
                     VAGLLIASVGTGWVFLINALSFVAVLGSLRMLRISELNLKPRAVRTRGSFVEGFKYVW
                     TRPDLKAALLMLFLIGAFGLNFPIFISTMSVTAFHAGASQYGVLSSTMAIGSVTGALL
                     AARRAKPRMAILIVAAALFGMGCTTASLMPNYVLFGLALVVIGISTQTFTTSTNSLVQ
                     ISTDPAMRGRVIAILLAISLGTTPFGAPVVGWVADRFGPRWALGVGAASGFAAAIVGL
                     IYLVRYRQLRVYVEARRLRYSIDDPRQGPAYVSPATAVQNAVLSEAEEDSSAGV"
     gene            complement(4195..5718)
                     /locus_tag="VHV99_17050"
     CDS             complement(4195..5718)
                     /locus_tag="VHV99_17050"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX3637157.1"
                     /translation="MNAVSEQAKLSVQQLGHYISGAPVASTSGRVKDVFNPATGQVTG
                     SVALASVEEVDAAVKAAKAAFPAWSETAPIKRARILFKFKELLNRHHDELAMLITREH
                     GKVFTDAQGEVVRGIEVVEFACGIPNLLKTDFTDQIGGGIDNWNLRQPLGVVAGITPF
                     NFPVMVPMWMFPVALACGNTFVLKPSERDPSASLRIAELLKEAGLPDGVFNVVNGDKV
                     AVDALIDHPDVAALSFVGSTPIAEYIYTEASKRGKRVQALGGAKNHLVVMPDADLDQA
                     VDALIGAAYGSAGERCMAISVAVAVGHVADELIERLVPRVKSLVIRNGEHLDAEMGPL
                     VTAEHKAKVAGYIDSGVAEGAKLVVDGRAHPVANEDGFFIGGTLFDQVGTGMKIYKEE
                     IFGPVLAVVRVPDFASAVELINAHEFGNGVSLFTSDGGVARAFGRQIQVGMVGINVPI
                     PVPMAWHSFGGWKRSLFGDHHAYGEEGVRFYTRYKSIMQRWPDSIAKGAEFTMPVAK"
     gene            complement(5873..6952)
                     /locus_tag="VHV99_17055"
     CDS             complement(5873..6952)
                     /locus_tag="VHV99_17055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637158.1"
                     /translation="MSNDPASRPAERSRQSALKQGVFLHAGWRSAGTWIWSRLRTLDS
                     VTGFYEPLSNVLADLSLADISASRPTLTSGHPPLAAPYFEEYRPFLQEGVRGVAGYRK
                     RFGTDRFSNAPDAEFPALQAYLANLCERTTGHGSVPVLKFCRSSGRLPWLKRAFPQVT
                     HAAMLRNPASQFASGWLLNQQWSNPFFVAAPFRVLGLNQSEPLVRQAIEICGVSLPPV
                     VPASDDAYAMACEHYARTAEGNNAYRAFVTLWILCVLRMADGVDLVIDVDRLGRSRDY
                     AAALRAEFLAQSGLSPDFGSARDLVEETRRSAARMAGIDGRSMRAVHSAALKFLKVQG
                     GADAAFTELVREKMVLANELTEQWR"
     gene            6951..7097
                     /locus_tag="VHV99_17060"
     CDS             6951..7097
                     /locus_tag="VHV99_17060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637159.1"
                     /translation="MVVDPPVALDGSLDGPCDNGIGLLFNWKRQRMDGRLALPADTLL
                     KRTF"
     gene            7201..7278
                     /locus_tag="VHV99_17065"
                     /pseudo
     CDS             7201..7278
                     /locus_tag="VHV99_17065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866215.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase"
     gene            7606..7803
                     /locus_tag="VHV99_17070"
                     /pseudo
     CDS             7606..7803
                     /locus_tag="VHV99_17070"
                     /inference="COORDINATES: protein motif:HMM:NF025471.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7843..8295)
                     /gene="hpaR"
                     /locus_tag="VHV99_17075"
     CDS             complement(7843..8295)
                     /gene="hpaR"
                     /locus_tag="VHV99_17075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HEX3637160.1"
                     /translation="MSASATTRVLHRNLPMLLLRAREKMMERFRPLITAHGLTEQQWR
                     VIRALNEHGPMEPRHISDICTISSPSMAGVLARMESMELVTKERFAEDQRRVLVSLTD
                     TSVELVRVISKDLEAHYRELERKVGPEIVERVYRAVDDLLAGLEEEDE"
     gene            8428..9207
                     /locus_tag="VHV99_17080"
     CDS             8428..9207
                     /locus_tag="VHV99_17080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX3637161.1"
                     /translation="MFALADHLLHPEGDALPRDVDARTAAALLARGVACRAPVSGAVY
                     GTLLNDRAALEALGDAVHAAPYKAPPKAPILYVKPRNTFAGHRARVVVPDNALGVEVG
                     ASLGIVISRTATRVSAGEAFNYIAGYTLVADLSVPHASVYRPSVRFRARDGFCVIGPA
                     VVAAHHVAAPDNLTIKVSLKGREASTFTASTASSIRGVAQLIADVTDFMTLSAGDVIT
                     LGVPYGSPVAHSGDTATLSIGAMPPLEVSFVDAEEHDGEQP"
     gene            9204..9962
                     /locus_tag="VHV99_17085"
     CDS             9204..9962
                     /locus_tag="VHV99_17085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX3637162.1"
                     /translation="MMRARVAYAGAIHEAYPDAHGVRLADGRVRREDEVVWLAPIEVG
                     TIFALGLNYAEHAKELQFNKQEEPLVFLKGPGAVIGHRGFTRRPADVTFMHYECELAV
                     VIGQSAQNVKRENAMQYVAGYMIANDYAIRDYLENYYRPNLRVKNRDGGTVLGPWFVD
                     AADVEDVTQLELRTFVNGTRQQHGNTRDLVTDIPALIEYLSSFMTLAPGDVILTGTPE
                     GIVNVKAGDEVACEIDGLGRQVNTIASDADFGRA"
     gene            10053..11510
                     /gene="hpaE"
                     /locus_tag="VHV99_17090"
     CDS             10053..11510
                     /gene="hpaE"
                     /locus_tag="VHV99_17090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HEX3637163.1"
                     /translation="MRIEHLINGKSGAAKDYFETVNPATQEVLAEVARGGAEEVDAAV
                     RAAKDAFPAWAAKPAAERAKIIRKLGELIAKNVPEISETETKDTGQTISQTRKQLVPR
                     AADNFSYFAEMCTRVDGHTYPTDTHLNYTLFHPVGVCALISPWNVPFMTATWKVAPCL
                     AFGNTAVLKMSELSPLTASMLGNLALEAGIPAGVLNVVHGFGKDTGEPLVAHPDVHAV
                     SFTGSTATGNRIVQTAGLKKFSMELGGKSPFVIFDDADFERALDAAVFMIFSNNGERC
                     TAGSRILVQRSIYARFAERFIERAKRLTVGDPLADSTIVGPMISQGHLAKVRSYIELG
                     PKEGATLACGGLDTPDLPDAMRKGNFVTPTVFVDVDNRMKIAQEEIFGPVACLIPFDD
                     EADAIKLANDISYGLSSYIWTESSGRALRVAAAVEAGMCFVNSQNVRDLRQPFGGTKA
                     SGVGREGGTWSYEVFLEPKNVCVSLGSHHIPRWGV"
     gene            11590..12453
                     /gene="hpaD"
                     /locus_tag="VHV99_17095"
     CDS             11590..12453
                     /gene="hpaD"
                     /locus_tag="VHV99_17095"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HEX3637164.1"
                     /translation="MGKLALAAKVTHVPSLYLSELDGPHKGCRQPAIDGHHEIGRRCR
                     ELGVDTIVVFDVHWLVNSEYHINCAPKFEGVYTSNELPHFIKNMPYAYPGNVGLGDLI
                     AEVANEMGVKSRAHSETTLELEYGTLVPMRYMNSDQHFKTVSVAGWCMWHDLETSARF
                     GLAVRKAIEERYDGTVAILASGSLSHHFANNGTAEQFMHKVWSPFLEQMDRKVVELWE
                     AGDWKTFCEMLPLYNEKCWGEGGMHDTAMLLGALGWDRYDGKVEVVTPYFGSSGTGQI
                     NAIFPVTPLPA"
     gene            12475..12873
                     /locus_tag="VHV99_17100"
     CDS             12475..12873
                     /locus_tag="VHV99_17100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014973072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HEX3637165.1"
                     /translation="MPHLTLEYSASLAGEESIGRLCKSLAHCLDAQRENEQRLYPLGG
                     IRVRALRCEQYCIADGRADAAFLHANLKIGAGRSEAAKKATGDALFELIKQHFAAEFE
                     KHGLALSLEINEFSEAGTWKHNNLHARLKA"
     gene            12935..13738
                     /gene="hpaH"
                     /locus_tag="VHV99_17105"
     CDS             12935..13738
                     /gene="hpaH"
                     /locus_tag="VHV99_17105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HEX3637166.1"
                     /translation="MLDEQTIRDLAAQLDHAEKTRTQLRHFSAKYPEMTVQDGYAIQR
                     EWVKLKLAEGHVIKGRKIGLTSRAMQRSSQIDEPDYAPLLDSMFIESGQDIRADRFIA
                     PRVEVELAFVLSKPLKGPGITLFDVLDATAYVTPAVEIIDARIEQFDRDTKASRKVYD
                     TISDFAANAGIVLGGRPVRPLDVDLRWVGALLYKNGAVEESGLAAAVLNHPATGVAWL
                     ANKIAPYDEVLNANDVILSGSFTSPIPARAGDTFHVDYGPLGGIGLNFI"
     gene            13749..14552
                     /gene="hpaI"
                     /locus_tag="VHV99_17110"
     CDS             13749..14552
                     /gene="hpaI"
                     /locus_tag="VHV99_17110"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HEX3637167.1"
                     /translation="MSLPQNTFKRALAEDKPQFGLWAALADAYVTELLATAGFDWLLI
                     DNEHAPNDVRSTLAQLQAVAAYASHPVVRPVRSDSALIKQLLDIGAQTLLLPMIDTAE
                     QAAEAVAATRYPPQGIRGVGSALARASRWNRIPDYLNTAADELCVLVQVETVQGMENL
                     PAIAAVEGVDGVFFGPSDLSASMGLLGKPGDASVREAICKGIETVRNAGKAAGVLAPD
                     SGIAAEYLAAGATFVAVGTDTGLLSRAAADLAAAYKRTAKTAAQVKGGY"
BASE COUNT         3005 a         4525 c         4445 g         2753 t
ORIGIN      
        1 gggttgactc gaagcgaaaa cagaatgatc atcacgcccg ggaaaaactg ggtcacgccg
       61 tagtacgcgg tattgatcag cgtgagcatc agattcggcg tcagcaaggt catcacgatc
      121 gaaaccagca ggtaggccac gatcaccatt ttcgcgctgg ctttctgctt gctctcgggc
      181 atcttcggca acagattgcg cgtcacgatc gggccgatgg cgaggcagat tcccgtcagc
      241 accaatagac cggacagcgc cgcgccggcc gcgaccagac ccagcagcca gcccggcagc
      301 aaacggaccg ccgccgcaaa gaatgcttca ttcggcgagg caaggtgcag gttctgactg
      361 atcgcatagt acgaagcaat caccagaaac ggatacatca gcatgtacag cggcatggcg
      421 acctgcgtgc ggcggatcgt attcggactg gccgccgtga agaaaacctg cacggcgaat
      481 ggcatcacgt agaaccctag cgactggaac aacatggtgc tcatcgagaa cgtcagttga
      541 ctcgcgttca tgctgttgct gacgtgcaaa ctcgcggcgt gaaaaacttc cgtcacgccg
      601 gattcccacg aaaccgcgag cccggtgatc acaatcgccg cgaccatcag tatgtctttc
      661 aggatcgcga tgtacgccga ggcccgcacg cctgaaatgg cgatgtaggt aaaggccagc
      721 accgctgaga tcagaatcag atagagcggc tggaaattcc agccaagtcc cttgaacgcg
      781 gccacgagcc ccgtgaattg caactccccc caaggcagca aaaacaggat cgccgagccg
      841 gcgacgatca actccagcgc gcggctgccg aaatggcctt tgaacaggtc gggcagcgta
      901 atggcgttat agcgtttgcc ggcctcccag atcttcggcc ctatgaaata gccgacggga
      961 tacgccagca ggatatatcc caggaaccag accccgtaag tgggcccctt ggcatagatg
     1021 ccgcctggaa agccgaccat cgtgccgata ctgtagattt cgcccgcagc gaggaaaaag
     1081 accagatacg cgccaaactg ccgggacgcg acaaagaagt catgaacgct ttgcgcacca
     1141 tgcccctttt ttgaccgtat cgccagatac agcgagaaga gaatcaaacc gagaaagaca
     1201 acggtggaca tgcggactct cctgctgcgt cagtgggttt tcggatgcgc atcgtcggcg
     1261 cggcgatcga acagacgcca gcacacctgc aggcacagcg acgtcagaac gaaccacgcg
     1321 aaaatccacg cgtagatgaa cggcacaccc agaacgaatc gatcgactga cgcgacccag
     1381 ggcagcaggc cgatcacgcc gatataggga atgccgatcc caatcaggaa cttgagcatg
     1441 gagtctccgt ctgcgtttcg tgttcgatga aatcatctct gccgaatccg cctgccattc
     1501 aacgaccgca ccggatggcg gcggtccgcc gcgagcggat agggcacgac ggccatagca
     1561 gacgatttca gtatcggtgg ccgaaaatac acctggctag taccagcgac aataattcgc
     1621 ctggtccaca cgagagagcc gaagaccggg cacgaattac gctcacgctg cattacgccc
     1681 tccttccgtc aacttgactc ccggcacatg cgaaggtaac ctacgaaggt aaccttcata
     1741 tctgccggta tggccagcaa cgacactcag cccacacgta ccccctccga tgccgacgcg
     1801 ctgcacgcaa cggcggaaga cctgcgcgtc ctggtcggca aattgcgtcg ccggttgcgc
     1861 gaagaagcgc atgtgggcga tttcacgccg tcacaagtac aggttctcgg cctgctcgaa
     1921 cgcgaaggtc ccgcaacggt cacgacgctc gcgcgcatcc agggtatgcg tccgcaatcc
     1981 atgggcgaga cgctttcggt tttgaaagct gccgggctcg tgagcggcgc accggatccg
     2041 aacgacgggc ggcaaaccgt tctttccctc acgcccgcct tccgcaagaa ggtcaaggca
     2101 agccgcacgg cacgcgaaga ctggctattc cgtaccatcc agacccgctt cagcgccgct
     2161 gaacagaaac aactcgcgat tggtatcgac ctgctcaaac gcctcatcga tttgtaactt
     2221 ccaggaaaag ctcatggctc tcaccacgct cgacgcaaag accgcattgc tcgtcatcga
     2281 tttgcagcga ggcattgttg cgctgcccac cgcccatccc accgccgagg tcgtgacacg
     2341 ctccgcctca ctggccgacg cgtttcgtcg ccacggtctg ccggtggtgc tcgtcaacgt
     2401 tgcaggcggc gcaccgggcc gcgcggaaca gtcgcgccat accggcgatt tccccgctgg
     2461 ctttgccgat ctcgtacccg aagtgaatca gcagccgtcg gatcatctgg tcaccaaacg
     2521 cacctggggc gcgttcaccc agacggatct cgaagcgtat ctgcgcaagc aaggcgtcac
     2581 gcaagtggtg ctggtcggcg tggccaccag catcggcgtc gagtccaccg cgcgttttgc
     2641 ccacgaactc ggcttcaacg tcactttcgc ggtcgacgcg atgacggaca tgaacctcga
     2701 cgcgcacacc aacagtatcg cgcgaatttt cccgcgtctc ggcgaaagcg gcacgacgca
     2761 ggacgtcctc gatctgctcg agaaatcgcg cgcatgagca tgcgttcgaa ccgctgcgcg
     2821 acgcggatcg tccacaccgg aatgtcccga tgagcggcac attccgctcg ctgcgcacgt
     2881 tcaactaccg ggtctgggcc agcggcgcga tcgtctccaa catcggaaca tggatgcagc
     2941 gcacggcgca ggactggctc gtgctcacgg aactcacgca tcacaatgcc acctccgtgg
     3001 gcattgtgat gtcgctgcag ttcgggccgc aaatgctcct gctgccctta acaggctatg
     3061 cggccgatca tttcgaccgc cgcaagctgc tgttcgccac gcaggcggcg atgggcacat
     3121 tggcgctgtg cctcgggctt cttacggtga ccggcctcgt gcggttatgg caggtctata
     3181 tgtttgcggg gctgcttggc tgcgttaccg cgttcgattc accggcgcgt cagacctttg
     3241 tctccgatct ggtgggcgag gccgatctgt cgaacgcggt cgcgttgaat tccacctcgt
     3301 tcaacgcggc gcgcatgatc gggcccgccg tcgcggggct attgatcgcc tcggtgggca
     3361 ccggctgggt gtttctgatc aatgcgctct ctttcgttgc cgtgctcggt tcactgcgca
     3421 tgctgcgcat cagcgaactg aatctgaagc ctcgtgcggt acgtacacgg ggcagtttcg
     3481 tggagggatt caagtatgtg tggacgcgtc ccgatctgaa ggccgcgctg ttgatgctgt
     3541 ttttgatcgg cgccttcggg ctgaacttcc cgatcttcat ctccaccatg tcggtcaccg
     3601 cctttcatgc gggcgcaagc caatacggcg tgttgagctc gaccatggca atcggttcgg
     3661 tgaccggggc gctgctggcc gcgcgcagag cgaagcctcg catggctatt ctgatcgtcg
     3721 cagcggcgct tttcggaatg ggctgtacga ccgcctcgct catgccgaac tatgtgcttt
     3781 tcgggcttgc gcttgtcgtg attggcatat ccacgcagac gttcaccacc tcgacgaaca
     3841 gtctcgtgca aatctccacc gacccggcca tgcgtggccg ggtgattgcg atcctgctgg
     3901 ccatctcgct aggcaccaca ccgttcggcg cgccggtggt gggctgggtg gcggatcgtt
     3961 tcggcccgcg ttgggcgctc ggcgtgggcg ccgcatcggg tttcgcggcg gcgatcgtcg
     4021 ggctgattta tctggtgagg tatcgccagt tgcgtgtgta tgtggaggcg cgtcgcttgc
     4081 gctatagcat tgatgatccg cgccaggggc cggcttatgt cagtccggca actgcggtgc
     4141 agaatgccgt gctgagcgag gcggaagagg attcgtccgc tggggtttga gggcttactt
     4201 cgcgaccggc atcgtgaact cagcgccctt cgcgatgcta tccggccagc gctgcatgat
     4261 gctcttgtag cgcgtataga aacgcacgcc ttcctcgcca tacgcgtggt gatcgccgaa
     4321 cagcgagcgc ttccagccgc caaacgagtg ccatgccatc ggcaccggaa tcggcacatt
     4381 gatgccgacc atgcccacct gaatctgccg cccgaacgcg cgcgccacgc caccatccga
     4441 cgtgaagagc gacacgccgt taccgaattc gtgcgcgttg atcaactcga ccgcgcttgc
     4501 gaaatcgggc acacgcacca cggccagcac cgggccgaag atttcttcct tgtagatctt
     4561 catcccggtg ccgacctggt cgaacagcgt accgccgatg aagaagccgt cttcattcgc
     4621 gaccggatgc gcgcggccat cgacgaccag tttcgcgcct tccgccacgc ccgagtcgat
     4681 ataaccggcc accttggcct tatgttcggc ggtaaccaac ggacccattt cagcgtcgag
     4741 atgttcaccg ttcctgatca ccagcgactt gacgcgcggc accaggcgtt cgatcaattc
     4801 atcggcgaca tggccaaccg ccaccgcgac cgagatcgcc atgcaacgct cgccagccga
     4861 accgtaagcg gcgccgatca aggcgtcgac tgcctggtcc agatcggcgt ccggcatcac
     4921 caccagatga ttcttcgcgc cgccgagcgc ctgcacgcgc ttgccgcgct tcgacgcttc
     4981 cgtatagatg tattcggcga tcggcgtgga gccgacaaac gacagcgccg cgacgtccgg
     5041 atggtcgatc agtgcatcca cggccacctt gtcgccgttc acgacgttga acacgccatc
     5101 gggcaaacca gcttccttca gcagttcggc aatccgcagc gaggccgacg ggtcacgctc
     5161 cgagggcttc agcacgaacg tgttgccgca cgcgagcgcg accgggaaca tccacatcgg
     5221 caccatcaca gggaaattga acggcgtgat gcccgccacc acgcccagcg gctgacgcag
     5281 attccagttg tcgatgccgc cgccgatctg gtcggtgaaa tcggtcttca gcaggttcgg
     5341 gatgccgcag gcgaactcga ccacttcgat gccgcgcacc acttcgcctt gcgcgtcggt
     5401 gaacaccttg ccgtgttcac gcgtgatcag catggcgagt tcgtcgtgat gcctgttcag
     5461 cagttccttg aacttgaaca gaatgcgcgc ccgcttgatg ggcgcggttt cactccaggc
     5521 cggaaaggcg gctttggccg ccttgaccgc cgcgtccact tcctcgaccg aagccagggc
     5581 gaccgagccg gtcacctggc cggtggcggg attgaacacg tccttgaccc ggccgctcgt
     5641 cgacgcaacc ggcgcgccgc tgatgtaatg acccaactgt tgcaccgaca actttgcttg
     5701 ttcgctcact gcattcattt cgttacctcg tgttcgggat aggcggcagg attggcaaac
     5761 tgtatcgacg gtgcccgtca tattcccgat acagcacaat aaaaccgcgc ctcactgtat
     5821 tgtttttcgc ttggcaactg tattggctgg cgacgtcgct gctggcgaac gttcagcgcc
     5881 attgctcggt caattcgttg gcgagcacca tcttctcgcg aacgagttcg gtaaaggcgg
     5941 cgtccgctcc gccctgcacc ttgaggaatt tcagcgccgc ggagtgaacg gctcgcatcg
     6001 accggccgtc gatcccagcc atgcgcgccg cgctgcgacg cgtttcctcg accagatcgc
     6061 gcgcgctgcc aaagtcgggc gacaagccgc tctgcgccag gaactcggcg cgcaacgcgg
     6121 ccgcatagtc gcgcgaccgc cccaagcggt cgacgtcgat caccagatca acaccgtccg
     6181 ccatccgcag cacacagaga atccacaacg tgacgaacgc gcgatacgcg ttgttgcctt
     6241 ccgccgtgcg cgcatagtgc tcgcacgcca tggcataggc gtcatccgaa gccggcacga
     6301 caggcggcaa gctcacgccg cagatttcga tggcctgccg gaccaaaggc tccgactgat
     6361 tcagacccag cacgcgaaac ggtgcggcca cgaagaacgg gttgctccat tgctgattga
     6421 gcaaccaccc cgaggcaaac tgcgaggcgg gattgcgcag catggccgca tgcgtgactt
     6481 gcgggaatgc gcgcttgagc cacggcaagc ggcccgacga acggcaaaac ttcaacacgg
     6541 gtacgctgcc gtgcccggta gtacgctcgc acaggttggc cagataggcc tgcaacgcgg
     6601 ggaattccgc gtcgggtgca tttgagaagc gatccgtgcc gaaacgcttc cggtatccgg
     6661 ctacgccgcg cacaccctct tgcagaaatg gccggtactc ctcgaagtac ggcgcggcga
     6721 gcggcggatg tcccgaggtc agcgtaggac gggacgcgga aatgtcggcg aggctcagat
     6781 cggcgagcac gttgctgagc ggttcataga agccggtcac ggaatcgagt gtgcgcaggc
     6841 gcgaccagat ccacgttccg gcgctgcgcc agcccgcgtg cagaaatacc ccctgtttca
     6901 gggcactctg ccgcgagcgc tcggctgggc gggaagctgg atcattcgac atggtggtcg
     6961 atccgccagt ggcgctggac ggatcgctcg atggcccctg tgacaacggc attggacttc
     7021 tcttcaattg gaagcgacaa cgaatggatg gacggctggc gctgcctgcc gatacgctgc
     7081 taaagcgcac attctagcgg ctctggcttt cactcgcgga tgcgccgcta cccgtgcgca
     7141 cgggaacctc gcgcccaaac gctttaagct gtcaggcccc cgaactcaaa agttgccgcc
     7201 atgaacgatt cgtggacagc gtggaatcac gccgcgggtt ccgagcagat gggccggacg
     7261 tggcgtgccg cgtatttggc tcagccgttt cggcatggac caaagccact ccatattccg
     7321 gtgcacgcat gacgttttca gggttggtga aggagatatc gcatgattca tacaatgcaa
     7381 gatgaaaaca agttgagggg agacgacaac gtcatctggc atgacaacgg agacgtgtat
     7441 ttcaacggca caaagatcga caacatgcat gtctatgcac cttaaggaac aagccacaat
     7501 gtcaacgcac cacgccttgc atacgcgtcc ttttcaatct atcaggatgc cgaaccccca
     7561 gagttttgga ccacgtattt caatgtggcg ccagatcgtt ccggcgtgaa gggtgaactc
     7621 aaggttatgc cctcgggccg tgtcagcgaa aacagatggc gattcggcta ctggaacgtc
     7681 agcagtgaaa cagccgtaat gagtgacaaa cttgagccgc acctgaggta cctggttgat
     7741 cggcttggcc tgcctcggga tgacctgcgc gcgctcatcg agagaaccgg cgcgcgaatg
     7801 cggttctttt gttactggca caacgagaca ggcgaacgtg cctcactcat cctcttcttc
     7861 aagccccgcc agcagatcgt cgaccgcgcg atacacgcgc tcgacgattt ccggccccac
     7921 cttgcgttcc agctcgcgat aatgcgcctc gagatccttc gagatcacac gcaccagctc
     7981 cacactcgta tcggtgagcg acaccagcac gcggcgctgg tcttccgcga aacgctcctt
     8041 cgttaccagt tccatgctct ccatgcgcgc gagcacccca gccatgctcg ggctcgaaat
     8101 cgtgcagata tcggagatgt ggcgcggctc catcgggcca tgctcattga gcgcgcgaat
     8161 cacgcgccat tgctgttcgg tcagcccgtg cgcggtaatc aacgggcgga aacgctccat
     8221 catcttttct ctggcgcgca gcaacaacat cggcaggttg cggtgcaaaa ctcgggtagt
     8281 agcggaagcg gacatggcgg ataagttgaa agcagccgtc gaaacttaag ggaaaacccg
     8341 cgatcagccg acaggctatt gaccgtggat gatatcaagc gcaatactac taacatgtta
     8401 gtgttttctc cgagagacag ctaatttatg tttgcacttg ccgatcacct gctgcatccc
     8461 gaaggcgacg cgctgcctcg cgacgtggat gcgcgcaccg ccgccgcgct gctcgcgcgc
     8521 ggcgtggcct gccgcgcgcc ggtttcgggc gccgtctatg gaacgttgct gaacgaccgg
     8581 gccgcgctgg aagcattggg cgacgcggtg catgccgcgc cgtacaaggc accgccgaaa
     8641 gcgccgattc tctatgtaaa gccacgcaac acgttcgccg gccatcgcgc acgtgtcgtc
     8701 gtgcctgaca atgcattggg cgtggaagtc ggcgcttcgc tgggtattgt gattagccgc
     8761 actgcaacac gcgtcagcgc gggcgaggcg ttcaactaca tcgccggcta cacgctggtc
     8821 gccgatctga gcgtgccgca cgcgagcgtg taccggccct cggtgcgatt ccgggcgcgc
     8881 gacggttttt gcgtgatcgg accggcggtg gttgcagcgc atcatgttgc ggcgccagac
     8941 aatctcacga ttaaggtttc gctcaaagga cgtgaagcct ctacttttac tgccagtact
     9001 gcatcgtcga tacgcggtgt ggcccaattg attgcggacg tcaccgattt catgacactc
     9061 agcgccggcg acgtcattac gctgggcgtg ccttatggtt cgccggtggc tcatagcggc
     9121 gacacggcca cgctatcgat tggcgcaatg ccgccgctcg aagtgtcgtt tgtcgatgcg
     9181 gaagaacacg atggagaaca gccatgatgc gcgcccgtgt tgcgtatgca ggcgcgattc
     9241 acgaagccta tccggacgcc catggcgtgc gtctcgccga cggtcgcgtg cgtcgtgagg
     9301 acgaagtggt gtggctcgcg ccaatcgagg tcggcacgat cttcgcgctc ggcttgaact
     9361 acgccgagca tgcaaaggaa ttgcagttca acaagcagga agagccttta gtctttctca
     9421 aaggtccggg cgcggtgatc ggtcaccgcg gcttcacgcg ccgccccgcc gacgtcacct
     9481 tcatgcacta cgagtgcgag ctggccgtcg tgatcgggca aagcgcgcag aacgtcaaac
     9541 gcgaaaacgc catgcagtac gtggccggct acatgatcgc caatgactac gccatccgcg
     9601 actacctgga aaactattac cgccccaacc tgcgtgtgaa gaatcgcgac ggcggcacgg
     9661 tgctcggccc ctggtttgtc gatgccgccg atgtcgaaga cgtcacgcaa ctcgaattgc
     9721 gcacgttcgt caacggcacg cggcagcaac acggcaacac gcgcgatctc gtcaccgata
     9781 tcccggcgtt gatcgaatat ctcagcagct tcatgaccct cgcacccggc gacgtaatcc
     9841 tgaccggcac gccggaaggc atcgtcaacg tcaaggcggg cgacgaagtg gcttgcgaaa
     9901 tcgacggcct gggccgtcaa gtcaacacga tcgcatccga cgcggatttt ggccgcgcct
     9961 gatcgactgc tttgcatggg atcggcgtag gcggtatggg actagagtag cctcccactc
    10021 cgatcgatag cgaaaagaga aaggacaaga caatgcgaat cgaacatctg atcaacggca
    10081 aatcgggcgc cgcgaaagac tacttcgaga ccgtcaatcc ggcgacacaa gaggtgctcg
    10141 ctgaagtcgc ccgcggcggg gcggaagaag tcgacgccgc cgtgcgcgcg gccaaagatg
    10201 cgtttccggc atgggccgcc aaacccgccg cggaacgcgc aaaaatcatt cgcaagctag
    10261 gcgagttgat cgcgaagaat gtgcctgaaa tctcggagac cgagacgaag gacaccggcc
    10321 agacgatctc gcagacgcgc aagcaactcg tgccgcgcgc cgccgacaat ttcagctatt
    10381 tcgcggaaat gtgcacgcgt gtcgatggcc atacgtaccc taccgacacg catctgaact
    10441 acacgctgtt ccatccggtc ggcgtgtgcg cgctgatctc gccgtggaac gtaccgttca
    10501 tgacggccac gtggaaagtc gcaccgtgcc tcgcgttcgg caataccgcc gtgttgaaga
    10561 tgagcgaact gtcgccgctc accgcgtcga tgctcggcaa tctcgcgctc gaagccggta
    10621 ttcccgccgg cgtgctgaac gtggtgcacg gcttcggcaa agacaccggc gagccgctgg
    10681 tggcgcaccc ggatgtgcat gccgtgtcgt tcaccggctc aaccgcaacc ggcaaccgca
    10741 tcgtgcaaac cgccggcctg aagaagtttt cgatggaact gggcggcaag tcgccgttcg
    10801 tgattttcga cgacgccgat ttcgaacgcg ccctcgatgc ggccgtgttc atgatcttct
    10861 caaacaacgg cgaacgctgc acggcgggtt cgcgcattct ggtgcaacgc tcgatttacg
    10921 cgcgctttgc cgagcgtttc atcgagcgcg caaaacgctt gacggtgggc gatccgctgg
    10981 ccgacagcac gatcgtcggc ccgatgatca gccagggaca cctcgccaag gtgcgcagct
    11041 atatcgaact cggtccgaaa gaaggcgcca cgctcgcatg cggcggactc gatacgcctg
    11101 acctgcccga cgccatgcgc aaaggcaatt tcgttacgcc aacggtgttc gtcgacgtcg
    11161 ataatcgcat gaaaatcgcg caggaagaga tcttcggccc agtcgcctgc ctgattccgt
    11221 tcgacgacga agccgacgcg atcaaactcg ccaacgatat ctcctacggt ttgtcgagct
    11281 acatctggac cgagagcagc ggccgcgcgc tacgtgtcgc ggcggccgtc gaagcgggca
    11341 tgtgcttcgt caatagccag aacgtgcgcg atctgcgcca gccgttcggc ggcacgaagg
    11401 catcgggtgt gggccgcgaa gggggcacct ggagctacga agtgttcctc gaaccgaaga
    11461 acgtgtgcgt atcgctcggc tcacatcaca tcccgcgctg gggcgtttga ggcgctttcg
    11521 catttagcgc cgtgcgcgag tgcggacggg caaactcagg cctcgcgcca tacagaatag
    11581 gagaccgcga tgggcaagct cgcattggca gcaaaagtca ctcacgtccc gtcgttatat
    11641 ttgtccgaac tcgacggtcc gcataagggt tgccgtcaac ccgccatcga cggccaccat
    11701 gaaatcggcc ggcgctgccg cgaattgggt gtggatacga ttgtcgtatt cgacgtgcat
    11761 tggctggtga acagcgaata ccacatcaat tgcgcgccga agttcgaagg cgtctatacg
    11821 agcaacgagc tgccgcattt catcaagaac atgccgtacg cgtatcccgg caatgtcggg
    11881 cttggcgatc tgatcgcgga agtcgccaac gaaatgggcg tgaaaagccg cgcgcatagc
    11941 gaaaccacgc tcgagctcga gtacggcacg ctcgtgccga tgcgctatat gaatagcgat
    12001 cagcacttca agaccgtgtc ggtagcgggc tggtgcatgt ggcacgacct cgaaaccagc
    12061 gcgcgtttcg gcctcgcggt acgcaaggcg atcgaggaac gttacgacgg caccgtggcg
    12121 attctggcga gcgggtcgtt gagccaccac ttcgccaata acggcaccgc cgaacagttc
    12181 atgcacaagg tgtggagtcc gtttctcgaa cagatggacc gcaaggtggt cgagttgtgg
    12241 gaagccggcg actggaagac cttctgcgag atgctgccgc tttataacga gaagtgctgg
    12301 ggcgaaggtg gcatgcacga caccgcgatg ctgcttggcg cgctcggttg ggatcgctac
    12361 gacggcaagg tggaagtcgt cacgccgtat ttcggcagtt cgggcaccgg ccagatcaat
    12421 gcgatcttcc ctgtgactcc cctgcccgct taagtcctga gaaaggagcg taccgtgccg
    12481 catttgacac tcgaatatag cgccagcctc gctggcgaag aaagcattgg gcggctttgc
    12541 aaatcgctcg ctcattgtct cgacgcacag cgcgagaatg aacagcgcct ttatccgctt
    12601 ggcggcatcc gtgtgcgcgc cttgcgttgc gagcagtact gcatcgccga cggcagagcc
    12661 gacgccgctt ttctgcatgc gaatctgaag atcggcgcag gtcgctcgga agccgctaaa
    12721 aaagcgaccg gtgacgcatt gttcgaactg atcaaacagc acttcgccgc tgagtttgaa
    12781 aagcacggcc tggcgttatc gctggagatc aacgaattca gcgaagccgg cacgtggaag
    12841 cacaacaatt tgcacgcgcg gctgaaagcc tgatcccaga tctgatgctc aggcctggtg
    12901 tcccggcctg gcagcggttt atagagaatc catcatgcta gacgaacaga ctatccgcga
    12961 tctcgcggcg caactcgacc acgccgagaa aacgcgtacg caattgcgcc atttctccgc
    13021 caagtatcct gagatgaccg tccaggacgg ttatgcgatt cagcgcgagt gggtgaaact
    13081 caaactggcc gagggccatg tgatcaaggg ccgcaagatc ggcctgacgt cacgtgccat
    13141 gcaacgttcc tcgcagatcg acgaacccga ctacgcgccg ttgctcgaca gcatgttcat
    13201 cgaaagcggc caggacattc gtgccgaccg tttcatcgcg ccgcgcgtgg aagtcgagct
    13261 ggccttcgtg ttgagcaagc ccttgaaggg ccctggcatc acgctattcg acgttctgga
    13321 cgccactgcc tacgtgaccc cggccgtgga gatcatcgac gcgcgcatcg aacaattcga
    13381 ccgcgacacg aaggcgtcgc gcaaggtcta cgacacgatc tcggacttcg cggccaatgc
    13441 gggcattgtg ctgggcggcc gtccggtccg gccgctggac gtcgaccttc gctgggttgg
    13501 cgcattgctt tacaagaacg gcgccgtgga agaaagcgga cttgccgcgg cggtgctgaa
    13561 tcatccagcg actggcgtgg catggctcgc gaacaagatt gccccgtatg acgaagtact
    13621 gaacgcgaac gacgtgattc tgagcggctc gttcacgagc ccgattccgg cgcgagcggg
    13681 cgacacgttc cacgtcgact atggtccgct tggcggcatt ggtttgaatt tcatttgagt
    13741 gttccgacat gtctctaccg caaaacacat tcaagcgcgc gttggctgaa gacaagccgc
    13801 aattcggctt atgggcggca cttgccgacg cgtacgtcac cgagttactg gccacagccg
    13861 gtttcgattg gctgttgatc gacaatgagc acgccccgaa cgatgtacgc agcacgctgg
    13921 cgcaattgca ggcggtggcc gcgtatgcat cgcatccggt ggtgcgtccg gtgcgcagcg
    13981 atagcgcgtt gatcaagcaa ctgctcgata tcggcgcgca gacgttgttg ttaccgatga
    14041 tcgacaccgc ggaacaggcc gcggaagccg tcgcggcgac gcgttatcca ccgcagggca
    14101 ttcgcggcgt ggggagtgcg ttggcacggg catcgcgttg gaaccggata ccggactacc
    14161 tgaacactgc cgctgatgag ctgtgtgtgc tggtgcaggt tgagacggtg caggggatgg
    14221 agaatctgcc ggcgattgcg gctgtggaag gcgttgatgg tgtgttcttt ggtccgtcgg
    14281 acctgagtgc gtcgatgggg ttgttgggca aacctggcga tgcaagcgta cgcgaagcca
    14341 tatgcaaagg catcgagaca gtgcggaatg cgggtaaagc ggccggcgtg ctggcgccgg
    14401 attcggggat cgcggctgag tatctggcgg cgggcgcgac gtttgtggcg gtgggcacgg
    14461 atacggggtt gttgagtcgc gcggcggcgg atctggcggc ggcgtataag agaaccgcga
    14521 agacggctgc acaagtgaaa ggcggatact gacaattcgc catctgtacg cgcctgcgca
    14581 ttcacaaatc tcgataccgt gacgaggcct tgggcaaaat gccagatcct caagacaggc
    14641 gcaatgtctc gcgcccgctc ttgctggtac agaacgcgga atcaacacgg ggtattgatt
    14701 ccgcgttctg taccagcaag agcgggcg
//
LOCUS       DATASH010000627         3593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_695247,
            whole genome shotgun sequence.
ACCESSION   DATASH010000627 DATASH010000000
VERSION     DATASH010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695247"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..1006)
                     /locus_tag="VHV99_17115"
     CDS             complement(107..1006)
                     /locus_tag="VHV99_17115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX3637168.1"
                     /translation="MNLSLYAVTVLIWGTTWIAIKWQLGAVPPPVSIAWRFWIAALLL
                     FALLRIMRRPIWPPRAAWRFLFAQGLALFCLNFLCFYYAEQVVPSGLVAVVFSTAPLL
                     NSINGRLFMGRPLQPSAIAGAALGLVGIVCLFVQQMAGHLGDHAVWLGLAIAFLGTLC
                     FSAGNLLSSRMQSMGLHPFATNSWAMLIGASILTVGSALAGFSFAIEPDARYLGALAY
                     LAVFGSVIGFTAYLMLVGRIGPERAAYSTVLFPIVALAASTIFEGYRWSGLAVVGLLL
                     VVAGNLVAFDLTRRLFARRVRAS"
     gene            1168..2088
                     /locus_tag="VHV99_17120"
     CDS             1168..2088
                     /locus_tag="VHV99_17120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX3637169.1"
                     /translation="MNARPPATVIESTETPFGLQSVCHTLRSANANLERFAWLGDRLA
                     IAVWTRDTEEAETVYDRPGHHTLSCYLDGGYRTERQKMPGRYGAPSRLCALPGEHESR
                     WWVRGHMHFMHLYFLPEHFTQRAVQELDREPRELTLADRTYFEDAHIASLCQSLANED
                     WQDPDGRLRTNETAHQVLSLLLRAQGVRRTDASLKGGLSVATRRRLRDYIENHLAQPL
                     TLGELAGVAALSEFHLARMFRASFGLPPAAWIAQQRLERARTLLRTTRLPLAQVAEQC
                     GYANASHFSHRFRDSVGVAPTAYRQAISAS"
     gene            complement(2101..>3593)
                     /locus_tag="VHV99_17125"
     CDS             complement(2101..>3593)
                     /locus_tag="VHV99_17125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX3637170.1"
                     /translation="PRLNQAFSEIKALFDPDNRFNPDKIVRPPKMDDARNFRFAPGYE
                     VHRIETALDWSAWNVERDPLTGQETLPGSGSDLAGGLAKAVEMCNNNGHCRKFDAGTM
                     CPSYRVTKDEQHVTRGRANTLRLAISGQLGEAGLASDEVKDTLDLCVSCKGCKRDCPT
                     GVDMAKFKIEARAARVKSHGLRLRDKLVAFMPRYAATASRMPGLMALADNVPVLSAWF
                     KRSVGFAPERTLPRFRKSFLANASAARPVQGGALKEVLLFVDTFNNHMEPDNARAAQQ
                     VLEAAGYTVHFNTRQGERPVCCGRTFLAAGLVDEAKQEARRMLDLFKPFVERGVPIVG
                     LEPSCLLSLRDEFLHYGFGEEAQRLSKQAFLFEEFLVREEKAGRLQLALKPLAMRQAL
                     VHGHCHQKAFDAFTPVQTVLNWIPDLKVSTVESSCCGMAGSFGYEAEHYATSQAMAEL
                     SLLPAVRKIDGNTLMVADGTSCRHQIHDGAGVDAIHVARVLAMALQ"
BASE COUNT          722 a         1228 c         1040 g          603 t
ORIGIN      
        1 catcttcgac agcacgatgc gcagatgctc gtcagcactc accgtcgcct tgaactcgct
       61 cagatgctcg ccgcacacgc cttgccccgt gcgcgcggcg cgcccctcag gatgccctca
      121 cccgacgcgc aaagagtcgc cgcgtgaggt cgaatgccac cagattcccc gccaccacga
      181 gcagaagtcc gaccaccgcc aatccggacc agcgataacc ctcaaagatt gtcgacgccg
      241 ccagcgccac gatcggaaat agcaccgtcg aataggcggc acgctccggt ccgatgcgcc
      301 cgaccagcat caggtacgcg gtaaagccga tcaccgagcc gaacacggct agataagcga
      361 gtgcgccgag ataacgcgcg tcaggctcga tagcgaagga aaagccggcc agcgcgctgc
      421 ccaccgtcag gatgctggca ccgatcagca tcgcccagct attggtggcg aacgggtgca
      481 agcccatcga ttgcatccgg ctcgacagca gattgccagc cgaaaaacac aaggtaccca
      541 ggaacgcgat agcgagcccc aaccacaccg cgtgatcgcc caggtgtccg gccatctgct
      601 ggacgaacag gcacacaatc ccgacgagcc ccaacgccgc gccggcaatc gccgacggtt
      661 gcaaaggacg gcccatgaac agtctgccgt tgatcgaatt caacagcggc gcagtggaaa
      721 acaccaccgc gacgagacca ctcggcacca cctgctcggc gtagtagaag cacagaaagt
      781 tcagacagaa gagcgccaac ccttgtgcga agaggaagcg ccatgcggcg cgcggcggcc
      841 agatcggccg gcgcatgatg cgcagcagcg caaacagcag cagcgcggcg atccagaaac
      901 gccatgcaat cgacaccggc ggcggcaccg cccccagttg ccatttgatc gcgatccacg
      961 tggtgcccca gatcagaacg gtgacggcat agagcgagag attcatgaca aagcctgcag
     1021 cagcaaaagg gtggagtccc actatgctgc agcgccggtg gctgcacttg tccaggattg
     1081 cgcacttttt cggagggctg cgccgccagt gatggcacgc cgtatactgg cgcttaccct
     1141 gtcatcgaac acactccgct cgttttcgtg aacgccagac cgcccgccac cgtgatcgaa
     1201 tccaccgaaa cgccgttcgg ccttcagtcg gtctgtcaca cgctgcgcag cgccaacgcg
     1261 aatctggaac gcttcgcgtg gctcggcgac cggctcgcca tcgccgtctg gacccgcgac
     1321 accgaagaag ccgaaaccgt ctacgaccgg cccggtcatc acacactgtc gtgctatctg
     1381 gatggcggct atcgcaccga gcgtcaaaaa atgcccgggc gttatggcgc gccgtcgcgg
     1441 ctctgcgcgt tgccgggcga gcatgaatcg cgctggtggg tgcgcggtca catgcatttc
     1501 atgcaccttt acttcctgcc ggaacacttc acgcagcgcg ccgtgcagga actcgatcgc
     1561 gagccgcgcg aactgaccct tgccgatcgc acctatttcg aagacgctca catcgcgagc
     1621 ctgtgtcagt cgctcgccaa tgaagactgg caggacccgg acggccggtt gcgcaccaat
     1681 gaaaccgcgc atcaggtatt gagtctgctg ctgcgcgcac aaggcgtacg tcgcacggat
     1741 gcgtcgctca agggcggttt atcggtcgcg acgcggcggc gcctacgcga ctacatcgag
     1801 aatcatctcg cgcaaccgct cacactgggc gagcttgccg gtgtagcggc actgtccgaa
     1861 tttcatctgg cgcgcatgtt ccgcgcgtcg ttcggactgc cgcccgcggc gtggatcgca
     1921 cagcagcggc tcgaacgcgc ccgcacgctg ctgcgcacca cgcgactgcc cctcgcgcaa
     1981 gtcgccgaac aatgcggcta tgcgaacgcc agccatttca gccatcgctt tcgtgacagc
     2041 gtcggcgtgg cgccgacggc gtatcggcag gcgatcagcg cgtcatagca gccggcaccc
     2101 tcactgcaaa gccatcgcca gcacgcgcgc cacgtggatc gcgtcgacgc ccgcgccatc
     2161 atgaatctga tgccggcaac tcgtgccgtc cgcgaccatc agcgtgttgc cgtcgatctt
     2221 gcgcaccgcg ggcagcagtg agagttccgc catggcctgc gatgtggcgt aatgctcggc
     2281 ttcatagccg aagctgcccg ccatcccaca gcatgacgat tccaccgtcg acaccttgag
     2341 atcaggaatc cagttcagca cggtttgcac gggcgtgaag gcgtcgaagg ccttttgatg
     2401 gcagtggccg tgtaccagcg cctgccgcat ggccaacggt ttcaacgcaa gctgcaaacg
     2461 tccggccttt tcctcgcgca ccagaaactc ttcaaataga aacgcctgtt tcgacagacg
     2521 ctgcgcctct tcaccgaaac cgtagtgcag aaactcatcg cgcaacgaca gcaagcacga
     2581 cggctccaac ccgacgatcg gtacaccacg ctccacgaac ggcttgaaca gatcgagcat
     2641 gcgccgcgct tcctgcttcg cttcatcgac caaaccggcc gcgaggaaag tacggccgca
     2701 gcacactggc cgttcgcctt ggcgcgtatt gaaatgcacc gtgtaaccgg cggcttccaa
     2761 cacctgctgc gcggcgcgcg cgttgtccgg ctccatgtgg ttgttgaatg tgtcgacgaa
     2821 caacagcact tctttcagcg cgccgccttg aacgggtcta gccgccgacg cattcgccaa
     2881 aaacgacttc ctgaaacgtg gcaacgttcg ttcaggcgcg aaaccaaccg agcgcttgaa
     2941 ccacgccgac aacaccggca cgttgtcggc cagcgccatc aggcccggca tgcgactcgc
     3001 cgtcgccgca tagcgcggca tgaacgccac cagcttgtcg cgtagacgca ggccgtggct
     3061 tttgacgcgc gccgcccttg cctcgatctt gaacttcgcc atgtcgacgc ccgtcgggca
     3121 atcgcgcttg cagcccttgc atgagacgca caggtcgagc gtgtccttga cctcgtcgct
     3181 ggcgagtcct gcttcgccga gctggccgga aatcgccaga cgcaatgtgt tcgcgcgtcc
     3241 ccgcgtcaca tgttgttcgt ccttggttac gcgataactc gggcacatgg tgcccgcgtc
     3301 gaacttgcgg caatggccgt tgttgttgca catctcgacg gccttcgcga gaccgcctgc
     3361 cagatcgctg ccgctgcccg gcagcgtctc ctgtcccgtc aacggatcgc gttcgacgtt
     3421 ccatgcggac cagtcgagcg cggtttcgat ccgatgcact tcgtagcccg gcgcaaagcg
     3481 gaaattgcgc gcgtcgtcca tcttcggcgg acgcacgatc ttgtcgggat tgaagcgatt
     3541 atccggatcg aacagcgcct tgatctcgct gaacgcctgg ttcagacgcg gcc
//
LOCUS       DATASH010000628         3191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4456724,
            whole genome shotgun sequence.
ACCESSION   DATASH010000628 DATASH010000000
VERSION     DATASH010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4456724"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /gene="ppsR"
                     /locus_tag="VHV99_17130"
     CDS             <1..516
                     /gene="ppsR"
                     /locus_tag="VHV99_17130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase/phosphoenolpyruvate
                     synthase regulator"
                     /protein_id="HEX3637171.1"
                     /translation="ELKSSHAMGRGHQNADTEEYKNRIEAINFSLAHDDGQSNRNLAD
                     ADVILVGVSRSGKTPTSLYLAMQYGVKAANYPLIPEDFERGKLPTPLLAHRQKMFGLS
                     IDPQRLSEIRNERRPGSKYAAPENCRYEINEAEAMMRREGIKWLSSTHKSIEEIATTI
                     LQEIKLDRAAY"
     gene            complement(710..1492)
                     /locus_tag="VHV99_17135"
     CDS             complement(710..1492)
                     /locus_tag="VHV99_17135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018435528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX3637172.1"
                     /translation="MKAITSRDNPLYKRLKALAGSTHQQRRSGHALLEGFHLASAYLD
                     VAGQPEMCIVTEGALRHDEAQAIVSRIDEQRIVTLPDALFGQLSNVVHGVGIVLLVEK
                     LGTPLPEKLAQTCLVLDGVQDAGNVGSILRSAAAAGIQQVFCVPGTAYAWSSKVLRSG
                     MGAHFLLQIHEDVEAQTLIERLAVPVVITDSHGAEAIYDCDLSGPLAWVFGNEGAGVS
                     QTWRDAVSLRVTIPQPGGMESLNVAAAAAVCVFEQCRQQRGR"
     gene            complement(1555..2328)
                     /gene="rnhB"
                     /locus_tag="VHV99_17140"
     CDS             complement(1555..2328)
                     /gene="rnhB"
                     /locus_tag="VHV99_17140"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX3637173.1"
                     /translation="MTQARAPRRKAATIAGAAAQQVGLNFETPDDIICGVDEAGRGPL
                     AGPVVAAAVIFDPSKPMIRGLDDSKVLTAKKRDELYDKIVERALAYCIASATVEEIDT
                     LNILHATMLAMKRAVEGLSVVPTLVKIDGNRCPTLSIRSEAVIGGDALVKSISAASIL
                     AKVTRDRMLLELHQVHPVYGFNAHAGYGTPQHLAALREHGPCEHHRRSFAPVREAHLR
                     FGTGVPLPAADVIVVPGTLNDAVLDDAMLDDAFGERSGA"
     gene            complement(2325..>3191)
                     /gene="lpxB"
                     /locus_tag="VHV99_17145"
     CDS             complement(2325..>3191)
                     /gene="lpxB"
                     /locus_tag="VHV99_17145"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEX3637174.1"
                     /translation="PDFNFGLEHPLREAGIPTVHFVCPSIWAWRGGRIKKIAKAVDHM
                     LCVFPFETALLEKAGVAASYVGHPLADEIPLEPDTLGARRTLGLAESGPIIAVLPGSR
                     RSEIDLIGPTFFAAMEMMQHQEPGLRFVMPAATPALRDMLRPLVDAHPGLALTITDGQ
                     AQLAMTAADAILVKSGTVTLEAALLKKPMVISYKVPWLTGQIMRRQGYLPYVGLPNIL
                     AGRFVVPEILQHFATPQALAEATLKQLRDEANRRTLTEIFTEMHHVLKQNTAQRAAEV
                     VASVVEKRKARP"
BASE COUNT          581 a         1040 c         1023 g          547 t
ORIGIN      
        1 gaactgaagt cgagccacgc aatgggtcgc ggccatcaga acgcggatac cgaggagtac
       61 aagaaccgga tcgaggcgat caacttctcg ctcgctcacg acgacggtca atcgaaccgc
      121 aatctggccg acgccgacgt gattctggtc ggcgtatcgc gcagcggcaa gacgccgacg
      181 agcttgtatc tggcaatgca gtacggcgtg aaggcggcca actacccgtt gattccggaa
      241 gatttcgagc gcgggaaatt gcctacgccg ctgctcgcgc atcggcagaa aatgtttggc
      301 ttgtcgatcg atccgcagcg gctttcggag attcgcaacg agcgccggcc cggcagcaag
      361 tacgccgcgc ccgagaattg ccgttacgag atcaatgaag cggaagcgat gatgcggcgt
      421 gaagggatca agtggttgtc gtcgacgcat aagtcgatcg aagagatcgc gacgacgatc
      481 ctgcaggaga tcaagctgga ccgggcggct tattgaaatt ggtttagatc cgcaccgcgc
      541 gctcagctgg cgcgcggcgg ccgggctgtg tggctgtgtg gcatgtggca tgtgccatct
      601 gttatctgcc atctgccatc tgccatgcgt catgcgtcat gcgtcatgcg caggtcaatg
      661 tggccaggat gccgcggctt ggtcgatacc gcggctgcct gcccgcgtcc tacctaccgc
      721 gctgctggcg gcactgctcg aacacgcaga cagccgccgc agccgccaca ttcagcgact
      781 ccatgccgcc cggctgcgga atcgtgacgc gcagggagac cgcatcgcgc cacgtctgcg
      841 acacgcccgc gccctcattg ccgaacaccc atgcgagcgg cccgctcagg tcgcaatcgt
      901 agatcgcctc ggcgccgtgt gaatccgtaa tcaccaccgg caccgcaaga cgctcgatca
      961 gcgtctgcgc ctcaacatcc tcgtggattt gcagcaggaa atgcgcgccc atgcccgagc
     1021 gcaatacctt cgacgaccac gcgtacgccg tgcccggtac gcagaacacc tgctgaatac
     1081 cagcggccgc cgcgctgcgc agaatcgagc cgacgttgcc ggcgtcctgc acgccatcga
     1141 gcacgaggca ggtctgcgcg agcttctccg gcagcggcgt accgagcttt tcgaccagca
     1201 gcacaatccc gaccccatgc acgacgttcg acaactggcc aaacaatgca tcaggtagcg
     1261 tgacgatgcg ctgctcgtcg atgcgcgaca cgatggcctg tgcctcgtcg tgacgcagcg
     1321 cgccctcggt gacgatgcac atttcaggct gcccggcgac gtcgagatac gcgctcgcga
     1381 gatggaaccc ttcgagcagc gcgtggccgc tgcgacgctg ctgatgcgtc gagccggcca
     1441 gtgccttgag gcgcttgtag agcggattgt cccgcgaggt gatagctttc acaggcagcg
     1501 aagacagtga gaatgagaaa agagttggcg acgcggccgg gtcacgcgag gcgctcaggc
     1561 gccactgcgt tcgccgaagg cgtcgtcgag cattgcgtcg tcaagcacgg cgtcgttgag
     1621 cgtcccgggc acgacgatca cgtcggccgc cggcagtggc acgcccgtgc cgaagcgcag
     1681 atgcgcttca cgcaccggcg cgaacgagcg ccgatgatgc tcgcaaggac cgtgttcgcg
     1741 cagcgccgcg agatgctgcg gcgtgccgta gccggcatgc gcgttgaaac cgtagaccgg
     1801 atggacttga tgcaattcga gcagcatccg gtcgcgcgtg accttggcga gaatcgaggc
     1861 cgccgaaata ctcttgacca gcgcatcgcc gccgatcacc gcctcgctgc gaatgctcag
     1921 cgtggggcag cggttgccgt cgattttgac cagcgtgggc acgaccgaaa gaccttcgac
     1981 cgcccgcttc atcgccagca tggtggcgtg caggatgttc agcgtatcga tttcctcgac
     2041 cgtcgccgac gcgatgcaat aggccagcgc ccgctcgacg atcttgtcgt acagttcgtc
     2101 gcgctttttg gcggtgagca ctttggaatc gtcgaggccg cgaatcatcg gcttcgacgg
     2161 atcgaaaatc acggccgcgg cgaccaccgg gccggccaac gggccgcgcc ctgcttcgtc
     2221 gacgccgcag atgatgtcgt ccggcgtctc gaagttcagg ccgacctgct gcgccgccgc
     2281 gcctgcaatg gttgccgcct tgcggcgtgg tgcgcgcgcc tgggtcacgg ccgcgccttg
     2341 cgcttttcga cgacgctcgc caccacttcc gccgcacgct gcgcggtgtt ctgcttcagc
     2401 acgtgatgca tctccgtgaa gatttccgtc agcgtgcgcc ggttggcctc gtcgcgcaac
     2461 tgtttcaacg tggcctcggc gagcgcctgc ggcgtggcga agtgctgcag aatttccggc
     2521 acgacgaaac gccccgccag gatgttcggc aagccgacat acggcagata gccctggcgg
     2581 cgcatgatct ggccggtcaa ccagggcacc ttgtacgaga tcaccatcgg ctttttcagc
     2641 agcgcggctt ccagcgtcac cgtgccgctc ttcacgagga tggcatccgc cgccgtcatt
     2701 gcgagctgcg cctggccgtc ggtgatggtc agcgcgagac cgggatgcgc gtcgaccagc
     2761 ggccgcagca tgtcgcgcag cgcgggcgtg gccgccggca tcacgaaacg caaacccggc
     2821 tcctggtgct gcatcatctc catcgcggcg aagaacgtcg ggccgatcag atcgatctcg
     2881 gagcgccggc ttcccggcag cacggcaatg atcggaccac tttcggcgag acctagtgtg
     2941 cggcgcgcgc cgagcgtgtc gggttccagc ggaatctcat cggcaagcgg atggcccacg
     3001 taagacgccg cgacgccggc cttttccagc agcgccgttt cgaatgggaa cacgcacagc
     3061 atatggtcga ccgccttggc gatcttcttg atgcgcccgc cccgccacgc ccagatggac
     3121 ggacagacga agtgaacggt cggaatgccc gcctcgcgca acggatgctc gaggccgaag
     3181 ttgaaatcgg g
//
LOCUS       DATASH010000629         2606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3861084,
            whole genome shotgun sequence.
ACCESSION   DATASH010000629 DATASH010000000
VERSION     DATASH010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3861084"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..1597
                     /locus_tag="VHV99_17150"
     CDS             167..1597
                     /locus_tag="VHV99_17150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016344434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEX3637175.1"
                     /translation="MRFLGIDLGTASLKLAIVDENGHERAVAAVAYAVTSPFPGWAET
                     PVESWWNALGEAAARLPAHERELVNAIGFSGQMHGVVVTDENNQALRPAMLWPDTRAL
                     DLLDAWPAPQANPVAPGMAGPLLRWLAVNEPAVMSKMRWALQPKDWLRTALGGEVCTD
                     PSDACATALAEPSGVWDDALITEIGLRREWFAPLAPSYAAAGTLNAEGATLLGLRAGI
                     VLAVGAGDTACAALGSGLSADGDALLTTGSGGQIVVMSTDAPASVVGLHAYRAATDQW
                     YRMAAMQNVGVALEAVRGWLGYTNWPGAYDDAFSTPASTRLTFLPYVSGERSPWMNPS
                     AKGGWLGLGLGDTRGTMMRAAFEGVAFALRAGLDAIRLNAGPSAVVRTLRLAGGGSID
                     PRWRQLLADVLNAELHAVDCPNAAARGAAILSGVALGSWHAADLAALAPSATLVASPN
                     SANDDGDLALRHTRFIDLYQRNASWF"
     gene            1688..>2606
                     /locus_tag="VHV99_17155"
     CDS             1688..>2606
                     /locus_tag="VHV99_17155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008351284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HEX3637176.1"
                     /translation="MSEPTTSPTSHPDHPDFRSREFLLDHALRTMTFYHPHCMDPAGG
                     FYHFYEDDGTIYDRKSRHLVSSTRFVFNYAMAYKHFGLDEYRGGVLHGIEYLRGFHRN
                     PQTGGYAWTLNGRDVTDATNHCYGLAFVVLAYAKALEAGFEEARGYLEETWDLMETHF
                     WDAAHGLYKDEASADWVVSDYRGQNANMHACEAMLAAFEASQDGRYLDRAALLADNMV
                     NRQATLAGGLVWEHYKPDWSVDWEYNKGDKTNIFRPWGFQPGHQTEWAKLLLILDRHR
                     PDPWHVTRARELFDRALDLSWDHEHGGMVY"
BASE COUNT          447 a          798 c          852 g          509 t
ORIGIN      
        1 cgggcgaacg acaatttgcg tcgcgcctgg caaggcgtcc ggataccagg cgtggttatg
       61 atggttatct gccgtgccgc atagtctctt tttcagaggc gcggcgcggg cagcgggtaa
      121 acccgtcgca agccgttttt accgcttact ttttctgcga atctccatgc gatttcttgg
      181 catcgatctg ggcacggcat cgctgaaact cgctatcgtc gatgaaaacg gtcacgagcg
      241 tgctgtcgcc gccgtcgcgt acgcggtcac ctcgccgttt cccggctggg cggaaacgcc
      301 cgtggaatcc tggtggaatg cgctgggcga ggcagccgca cgcttgcccg cccacgagcg
      361 cgagctggtg aatgcgatcg gtttctcagg gcagatgcac ggcgtcgtag tcactgatga
      421 aaacaaccag gcgctgcgtc ccgcgatgtt atggcccgat acccgcgcgc tcgatttgct
      481 tgacgcgtgg ccggcgccgc aagcgaatcc tgtcgcgccc ggcatggcgg ggccgttgct
      541 gcgctggctt gcggtgaacg aacccgcggt gatgagcaag atgcgctggg ctttgcagcc
      601 caaagactgg ctgcgcacgg cgttgggcgg cgaagtctgc accgatccgt cggacgcctg
      661 cgccaccgcg cttgctgaac cttcgggcgt ctgggacgac gcgctcatca cggaaatcgg
      721 gctgcggcgc gagtggttcg cgccgctcgc gccgtcgtat gcagcggccg gcacgttgaa
      781 cgccgaaggc gcgacattgc tcggcctccg agcgggaata gtgctggctg tgggtgcggg
      841 cgacaccgct tgcgccgcgc tcggcagcgg tctctcggcc gatggcgacg cgttgctgac
      901 caccggcagc ggtggccaga tcgtggtgat gtcgacggat gctcctgctt cggttgttgg
      961 cctgcacgcg tatcgtgcgg cgactgacca gtggtatcgg atggctgcga tgcagaacgt
     1021 gggcgtggcg cttgaagcgg tgcgcggctg gctcggctac acgaactggc cgggcgctta
     1081 tgacgacgcg ttcagcacgc cggcgtctac gcgcctgacg ttcttgccgt acgtgagcgg
     1141 tgaacgttca ccgtggatga atccatcggc gaagggcgga tggctcggcc tcggtctcgg
     1201 cgatacacgt ggcacgatga tgcgcgcggc cttcgaaggc gtggcgttcg cgctgcgcgc
     1261 cggcctcgat gccattcgtc tgaacgccgg accctcggcc gtggtacgaa ccctgcggct
     1321 tgccggcggc ggctcgatcg atccacgctg gcggcaactt ctggccgacg tactcaacgc
     1381 cgaactgcac gcggtcgatt gcccgaacgc ggcggcccgc ggcgcggcaa ttctgagcgg
     1441 tgtcgcgctc gggtcctggc acgcggcgga tctggcggcg ctggcgccat cggcgacgct
     1501 ggttgcatcg ccgaatagcg cgaatgacga cggcgatctg gcgctccgtc atacgcgttt
     1561 catcgattta taccagcgta acgcgagttg gttctgacgc acgttttcga cctccgctta
     1621 cactaacgct cgccccaagc aaatccacga gaatccacga gacagcacga cgagcttcat
     1681 acgaaatatg tccgaaccta ccacgtcccc gacttcacat cccgaccatc ccgactttcg
     1741 ctcgcgtgag ttcctgctcg atcacgcgtt gcggaccatg acgttttatc acccgcactg
     1801 catggacccg gcgggcggtt tctatcattt ctacgaggac gacggcacga tctatgaccg
     1861 caagtcgcgg catttggtgt cgagcacgcg cttcgttttt aactatgcaa tggcctacaa
     1921 gcatttcggc ctcgatgaat atcgcggcgg cgtcctgcat ggcattgaat acttgcgcgg
     1981 gtttcatcgg aatccgcaga ccggcggtta tgcgtggacg ctcaacggcc gcgacgtcac
     2041 cgacgctacc aatcattgct acggccttgc gttcgttgtg ctggcttacg cgaaggcgct
     2101 ggaagcaggc ttcgaagaag cgcgcgggta cctggaagaa acctgggacc tgatggaaac
     2161 gcacttctgg gatgcggctc acggcctgta caaagatgaa gcaagcgcgg actgggtcgt
     2221 gtcggactat cgcggacaaa acgccaacat gcatgcctgc gaagcgatgc tcgctgcgtt
     2281 cgaagcgtcg caggatgggc gttatctgga ccgcgccgcg ctgctcgccg acaacatggt
     2341 gaatcgtcag gctacgctcg ccggcggcct ggtctgggag cactacaagc cggactggtc
     2401 ggtcgattgg gaatataaca agggcgacaa gaccaacatc tttcgtccat ggggattcca
     2461 gcccggccat cagacggaat gggcaaagct gctgctgatt ctggaccggc atcgccctga
     2521 cccgtggcat gtcacgcgcg cgcgcgaact gtttgaccgc gcgctggatc tttcgtggga
     2581 ccacgagcac ggcgggatgg tttacg
//
LOCUS       DATASH010000630         8632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4849943,
            whole genome shotgun sequence.
ACCESSION   DATASH010000630 DATASH010000000
VERSION     DATASH010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 8632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8632
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4849943"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..140)
                     /locus_tag="VHV99_17160"
     CDS             complement(<1..140)
                     /locus_tag="VHV99_17160"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX3637177.1"
                     /translation="MSGNTLGTLFTVTTFGESHGPAIGCVIDGCPPGMALNEADIQLE
                     LDR"
     gene            complement(359..2296)
                     /locus_tag="VHV99_17165"
     CDS             complement(359..2296)
                     /locus_tag="VHV99_17165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018435246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3637178.1"
                     /translation="MSDRPLPAARRAARPHEEHESQFRLLGQRRFAPFFWTQFLGAMN
                     DNVFKIGFTSLVTYQAARFSGVDPKTAAFLISAIFILPFVLLSATSGQIADKYDKAML
                     TRFVKSFEIVVMLIGGAGFWLHSAPLLYLCTFLMGVHSTVFGPVKYSYLPQHLSKSEL
                     VGGNGMVEMGTFVAILIGTIVGGVGAGFVEHGAVVLACACVGIALVGRLASNFVPVTA
                     APQPDLRINWNPVSETWRNLKLARENRTVFLSLLGISWLWFVGATFLSSFFSFAKNVL
                     SADPDVVTVLLGTFSIGIGLGSLMCEKLSKRRIEIGLVPLGSIGMSVFAVDLFFASHA
                     LPAAGHLLSVGEFLMRLAHWRVLADLFLLAMFGGLYSVPLYALIQSRSQPSHRARIIA
                     ANNILNSLFMIVSSLMAMALTAAGFSIPAIFLVTALLNIVVATYIYSLVPEFLLRFMA
                     WVLVHTFYRIRLVHAERIPEEGAAVLVCNHVSLVDAIVIMAESPRPIRFVMDHQIFKT
                     PFAGWVFRHAKAIPIAPAHQDPELLTRAYERCAEALAEGDLVCIFPEGKLTKTGEMNP
                     FRHGVTEIVRRTPAPVIPMALRGLWGSVFSRANDARWPRPIQKGVMSRLTLAVGEPID
                     PAEATPEALQQIVTELRGARK"
     gene            complement(2442..2906)
                     /locus_tag="VHV99_17170"
     CDS             complement(2442..2906)
                     /locus_tag="VHV99_17170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX3637179.1"
                     /translation="MRVSDILKVKGNTLFTVTPDTTLHDAVNTMAEHDIGSLVVMEYG
                     DLVGMLTFREIILTLSKHGGSLGTATIRKLMDDHPLTCTPETDVNEVRRMMLEHHVRY
                     LPVMESRTLMGVISFYDVAKAVVEEQGFENRMLKAYIRDWPEEQQEQQPAPR"
     gene            3146..4648
                     /locus_tag="VHV99_17175"
     CDS             3146..4648
                     /locus_tag="VHV99_17175"
                     /EC_number="2.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase"
                     /protein_id="HEX3637180.1"
                     /translation="MSANRFDTLALHAGAAPDPTTGARATPIYQTTSFSFRDSDHAAA
                     LFNMERAGHVYSRISNPTVAVFEERVAALENGAGAIGTASGQAALHLAIATLMGAGAH
                     IVASSALYGGSHNLLHYTLRRFGIETSFVKPGDPDAWRAALRPNTRMLFGETLGNPGL
                     DVLDIAAVAQIAHEHGVPLLVDSTFTTPYLLKPFDHGADFVYHSATKFLGGHGTTIGG
                     VLVDGGTFDFNASGRFPEFTEPYEGFHGMVFAEESTVAPFLLRARREGLRDFGACLHP
                     QAAWQLLQGIETLPLRMERHVANTRKVVEFLCTHPAVEAVAYPELPSHPDHALAKRLL
                     PRGAGAVFSFNLRGDRAAGRSFIETLSLFSHLANVGDARSLVIHPASTTHFRMDAAAL
                     AAAGIAEGTIRLSIGLEDPDDLIDDLKRALKAAQKASGTRVPGSGPSGSSGAAKNSPP
                     SSSAASAEGNLSNSSAPRALDNLSGSSTPQAPDNSSSPSADATKPRPESA"
     gene            4645..5490
                     /locus_tag="VHV99_17180"
     CDS             4645..5490
                     /locus_tag="VHV99_17180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3637181.1"
                     /translation="MILTVQGKPAYAYTGGKPFDPSLPTAVFIHGAEHDHSVWALQTR
                     YFAHHGFGVLAVDLPGHCRSAGPALTTVTAMADWLAALLEAAGVQRAFVAGHSMGSLV
                     ALDFAGRYPERVTHLALLATAMPMAVSDALLDAALHREPDAIEMVNQWSHSTLAAKPS
                     CPGPGFWLHGMNQRLMERVSANGETHLFHTDFTACNGYADGLTRAAEVRCPTRLIVGR
                     RDAMTPPRAAKALADALAQAGVPVDTVTLEAGHALMTEQPDATLDALFSFASKAAGTV
                     SYGGR"
     gene            5690..6010
                     /locus_tag="VHV99_17185"
     CDS             5690..6010
                     /locus_tag="VHV99_17185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mpo1-like protein"
                     /protein_id="HEX3637182.1"
                     /translation="MAQTAHTDHFASFAEFYPYYLSEHRNTVSRRLHFVGSLGVIGCL
                     AMALATGDWLWLPAAVICGYGFAWVGHFFFEKNRPATFRHPLYSLMGDWVMFKDICVG
                     KISL"
     gene            complement(6038..7351)
                     /locus_tag="VHV99_17190"
     CDS             complement(6038..7351)
                     /locus_tag="VHV99_17190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HEX3637183.1"
                     /translation="MLSRVRFDLDTLKRLAQFAAKRSSEERIPQVAGSLTFTTMLALV
                     PLATVAFALFTAFPIFSSFQMSLQIFLADHLMPAQLNSQIFNYLNQFASKAKGLTTIG
                     MIFLFVTAVMTMMTVESAFNVIWRVRKARPIAQRILVYWAIITLGPILIGVSLSISSY
                     LFTRSMAFSAAQHITPVIDWALTSAALPLTALAFTMLYVFLPNCRVEWRDAVIGGITA
                     AIAFELAKRGFGYYVRRIPTYTAVYGAFAAVPLFLLWMYLCWFIALAGAMIASALPAI
                     RIGQFHRPTFEGSDLFDSLELLARLSEARDAGTRGYTVQELSRMLRREMDTTIKLLQK
                     LEEIEWIARLQEDGLRPHFLLLANPAQITVEQLFDLFVIDRAELEYQLQLHATRVDGE
                     TLLAALENDKLKVTLAALLAARAASRATRAQENGQGASSMPHQAA"
     gene            7472..8074
                     /gene="wrbA"
                     /locus_tag="VHV99_17195"
     CDS             7472..8074
                     /gene="wrbA"
                     /locus_tag="VHV99_17195"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006052419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HEX3637184.1"
                     /translation="MKDILVLYYSRHGATRELALAIAHGVDSVPGMQARVRTVPPVST
                     VCEATQPDIPAEGPPYVELRDLEECAGLALGSPTRFGNMAASLKYFLDGTTPQWLAGA
                     LSGKPACVFTSTGSLHGGQESTLLSMMLPLLHHGMLIVGIPYTESALSTTQTGGTPYG
                     ASHFARAGTAGQGISADEKTLAIALGARVARAAASLSERT"
     gene            8176..8613
                     /locus_tag="VHV99_17200"
     CDS             8176..8613
                     /locus_tag="VHV99_17200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004542279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HEX3637185.1"
                     /translation="MIALTGLSVAWEWWLAPLRPGGSALVLKAVPLLLALPGVWRHRL
                     YTLQWASMLILLYFAEGIVRGWSDRGLSARLGWLEAALSIIFFVCTLVYVGPFKRAAK
                     QAAKQATQQTTQRATEQVTQQASKQATPDNTKPNAEQAEGGAR"
BASE COUNT         1680 a         2889 c         2617 g         1446 t
ORIGIN      
        1 cggtcgagtt caagctggat atcggcttcg ttaagcgcca tgccgggcgg gcagccgtcg
       61 atcacgcagc cgatagcggg gccgtgcgat tcgccgaagg tcgtgacagt gaaaagcgta
      121 ccgagcgtgt tgccggacat gggcgtcgtc caaagaaatg cggggaaggc cctattatgc
      181 cagcccccaa ggccccaggg ccgcagcgcg cgccggccta tgccttctgg acaagtctcc
      241 ggcggggcgg ggccgcaggt ggaagcaggc attcctgccg cgacacagac ccagccgcgc
      301 attccatgac gccgcagctt gcgcgcagac ccgatcggca agccgctatc cggccacgct
      361 acttccgcgc cccgcgcagt tcagtcacga tctgctgcag cgcttccggc gtcgcctcag
      421 ctggatcgat cggttcaccc accgccagcg tcagccggct catcacacct ttctgaatcg
      481 gccgcggcca gcgcgcgtcg ttggcgcgcg aaaacacgct gccccacagc ccgcgcagcg
      541 ccatcggtat caccggcgcc ggcgtgcggc gcacgatctc ggtcacgcca tggcggaacg
      601 gattcatctc gccggtcttc gtcagcttgc cctccgggaa gatgcagacc agatcaccct
      661 cagccagtgc ctctgcacag cgctcataag cgcgcgtcag caactccgga tcctgatggg
      721 ccggcgcgat cggaatcgcc ttcgcatggc ggaacaccca gccggcgaac ggcgtcttga
      781 agatctggtg gtccatcacg aagcggatcg gccgcgggct ctcggccatg atgacgatcg
      841 catcgacgag gctcacgtgg ttgcacacca gcacggccgc gccctcttcc ggaattcgct
      901 cggcatgcac caggcgaatc cggtagaacg tgtgcaccag cacccaggcc ataaaacgca
      961 gcaaaaactc gggtacgagc gaatagatgt atgtcgcgac gacgatgttc agcaacgcgg
     1021 tgacgaggaa aatcgccgga atgctgaaac ccgccgcggt cagcgccatc gccatcaatg
     1081 acgaaacgat catgaacagc gaattgagaa tgttgttcgc ggcgatgatg cgcgcccggt
     1141 ggctcggctg gctgcggctc tggatcagcg catagagcgg cacgctatag aggccgccga
     1201 acatggcgag caggaacaga tcggccagca cgcgccaatg agcgagccgc atgaggaatt
     1261 cgccgacgct cagcagatgc cccgccgccg gcaacgcgtg actcgcgaag aacagatcga
     1321 ccgcgaacac gctcatgccg atcgagccga gcggcacgag gccgatttcg atgcgccgct
     1381 tcgagagctt ttcgcacatc agggaaccca aaccgatgcc gatcgagaac gtgccgagca
     1441 acacggtcac gacatccgga tcggcggaca gcacattttt cgcgaagctg aaaaacgacg
     1501 acaaaaaagt cgcgccgacg aaccacagcc aggaaatccc cagcaggctc agaaacaccg
     1561 tgcggttttc gcgcgcgagc ttcagattgc gccaggtctc gctgaccgga ttccagttga
     1621 tgcgcagatc gggctgcggc gccgccgtaa ccggcacaaa gttcgacgcc agccgcccca
     1681 ccagcgcaat cccgacacag gcgcaggcta gcaccacggc gccgtgttcg acaaaacccg
     1741 cgcccacgcc gccaacgatc gtcccaatca ggatcgcgac gaaggtgccc atctcgacca
     1801 tcccgttacc gccaaccagt tccgacttcg acagatgctg cggcaggtac gaatacttga
     1861 ccgggccgaa cacggtcgaa tggacgccca tcagaaacgt gcacagatac agcagcggcg
     1921 cgctatgcaa ccagaaaccc gcgccgccga tcagcatgac gacgatttcg aagcttttga
     1981 cgaaacgcgt gagcatcgcc ttgtcgtact tgtccgcgat ctggccggag gtggccgaca
     2041 acagcacgaa cggcagaatg aaaatcgccg agatcaggaa cgccgccgtt ttcggatcca
     2101 caccggaaaa gcgcgccgcc tgataggtga cgagcgacgt gaagccgatc ttgaagacgt
     2161 tgtcgttcat cgcgccaagg aactgggtcc agaaaaacgg cgcgaaacgg cgctgcccga
     2221 gcaggcggaa ctgggattcg tgctcctcgt gtgggcgagc ggcgcggcgg gcggcaggta
     2281 acggacgatc gctcataaag ttcggggaac gggctaaaca atcacaaaag gggaaagagc
     2341 caggcacaaa aaaagcgcac gctttcgcgc gcgcttgacc gcattttcac actcacggca
     2401 acggcgcgaa cgccgcggtg agccgctgcc cgttaagtta gttagcgcgg cgccggttgc
     2461 tgttcctgct gctcttccgg ccagtcgcgg atgtacgcct tgagcatgcg gttttcgaaa
     2521 ccctgctctt cgaccacggc cttcgcgacg tcgtagaacg agatcacgcc catcagcgtg
     2581 cggctttcca tcaccggcaa atagcgcaca tgatgctcga gcatcatgcg acgaacctcg
     2641 ttgacgtctg tttcgggcgt gcaggtgagc ggatgatcgt ccatcaactt gcggatcgtg
     2701 gcagtaccga ggctgccgcc gtgcttgctc aaggtcagga taatttcacg aaacgtcagc
     2761 atgccgacga ggtcgccgta ttccatcacg accagtgagc cgatatcgtg ctcggccatg
     2821 gtattgactg cgtcgtgcag cgtggtatcc ggcgtaaccg tgaaaagggt gttgcctttg
     2881 actttaagaa tgtcgctgac tcgcatgatc tgtctccgtg atgccgctta agctgataat
     2941 gcctgaatga ctttcgatcc tagcggaaag gcccataaaa agaaagcggc ccgccggcca
     3001 aggcgcagcg ggcgtttgca cgctcagtcc ggcgccatgt ggaccgcgcg catcgggaaa
     3061 accgcgcttg aagcgattca cgccggtgat aggatggccg ccactttcac ctttcgcgag
     3121 gctgtccgtg gcctcgccgc ccaggatgtc cgccaaccgt ttcgatacgc ttgcgctgca
     3181 cgccggcgcc gcgcccgacc ccaccaccgg cgcgcgcgcc acgcctattt accagactac
     3241 gtcgttttcg ttccgcgact cggaccacgc cgcggcgctc ttcaatatgg agcgcgccgg
     3301 ccacgtctat tcgcgcatct cgaatccgac tgtggccgtg ttcgaggaac gcgttgccgc
     3361 gctggaaaac ggcgcgggcg cgatcggtac agcgagtggc caggctgcgc tccatctggc
     3421 gatcgccacg ttgatgggcg ccggcgccca tatcgtcgcc tccagcgcgt tgtacggcgg
     3481 ctcgcacaac ctgctgcact acacgctgcg gcgcttcggc atcgagacaa gcttcgtcaa
     3541 accgggcgat cccgacgcat ggcgcgccgc gctgcggccg aacacgcgca tgctgttcgg
     3601 cgagacgctt ggcaaccccg gcctcgacgt gctcgatatc gccgcggtgg cgcaaatcgc
     3661 gcacgagcac ggcgtgccgc tgctggtcga ctcgaccttc accacgccct acctgctcaa
     3721 accgttcgac catggcgccg acttcgtcta tcactcggca accaaattcc tcggcgggca
     3781 cggcaccacg atcggcggcg tgctcgtgga cggcggcacg ttcgacttca acgcgtccgg
     3841 ccgtttcccc gaattcaccg aaccgtacga gggctttcac ggcatggtat tcgccgagga
     3901 aagcaccgtc gcaccgtttc ttctgcgcgc gcgccgcgag gggttgcgcg acttcggcgc
     3961 ctgcctgcat ccccaggccg cctggcaact cctgcaaggc atcgagacgc tgccgctacg
     4021 catggaacgg cacgtcgcga atacgcgcaa ggtagttgaa ttcctgtgca cacaccccgc
     4081 cgtcgaagcc gtcgcctatc cggaactgcc atcgcatccg gaccatgcgc tcgcgaagcg
     4141 tctgctgccg cgcggcgcgg gcgcggtgtt cagcttcaat ctgcgcggcg accgcgccgc
     4201 cggacgcagc ttcattgaaa cgctttcgct gttttcgcac ctcgcgaacg tcggcgacgc
     4261 acgctcactg gtcatccacc ctgcatcgac gacgcacttc cgcatggacg ccgccgcact
     4321 cgccgcagcc ggcatcgccg aaggcacgat ccgcctgtcg atcggtctcg aagaccccga
     4381 cgatctgatc gacgacctca agcgcgcgct gaaagccgcg cagaaagcga gcgggacgag
     4441 ggtgccgggg agcggaccat ccggttcgtc cggcgcggcg aaaaacagcc cgcccagttc
     4501 atccgctgct tcggcggaag gcaacctttc caattcgtcc gccccgcggg ccctcgacaa
     4561 cctgtccggc tcatccaccc cgcaggcccc cgacaactca tccagcccat ccgctgacgc
     4621 aaccaaaccg cgcccggagt ccgcatgatc ctcaccgttc aaggcaagcc cgcctacgcg
     4681 tacaccggcg gcaaaccctt cgaccccagc ctgccgaccg cggtgttcat ccacggtgcc
     4741 gagcatgacc atagcgtgtg ggcattgcaa acgcgttact tcgcgcatca cggcttcggc
     4801 gtactggcgg tcgatctgcc cggccactgc cgcagcgccg gccccgcgct caccaccgtg
     4861 acagccatgg ccgactggct ggcagctctg cttgaggccg ccggcgtaca acgcgcattc
     4921 gtggccggcc acagcatggg ctcgctagtc gcactcgact tcgccggccg ctatccggag
     4981 cgcgtcacgc acctcgcgct gctcgccacc gccatgccga tggccgtctc cgacgcgttg
     5041 ctcgacgccg ccctgcatcg cgagcccgat gcaatcgaga tggtcaatca gtggtcgcat
     5101 tcgacgctcg ccgccaaacc gtcgtgtccg ggcccgggct tctggctgca cggcatgaac
     5161 cagcggttga tggagcgtgt ctcggcgaac ggcgagacgc acctgttcca caccgacttt
     5221 accgcgtgca acggctacgc ggacggcctc acacgtgccg ctgaggtgcg ttgcccgacg
     5281 cgcctgatcg tgggccggcg cgatgcgatg acgccgccac gcgcggccaa agcgctcgcc
     5341 gacgcgctcg ctcaagccgg cgtaccggtc gacaccgtga cgctcgaagc cggccatgcc
     5401 ctgatgaccg agcagcccga tgccacgctc gacgcgctgt tcagcttcgc ctcgaaggcc
     5461 gctggcactg tttcatacgg tggtcggtaa tcgttgcgag tcgtcacgat aagcgcaccc
     5521 gtgcagacgc gccatcggcc gaatggccag gttgcgcgca tggcccgata gccgcacaat
     5581 gactcaagcc tgtcctgttt cgagtgcgtt ccgaacatgt cggcacacgg cgaagccacc
     5641 tgggagcgac gggcacggaa tcgagtagcg tcgacattcg gaggccatga tggctcaaac
     5701 cgcgcacacc gatcacttcg cgagtttcgc ggagttctat ccgtactacc tgagcgagca
     5761 tcgcaacacg gtatcgcggc ggctgcattt cgtgggttcg ctcggcgtga tcggttgcct
     5821 cgccatggcg ctcgccaccg gtgactggct atggctgccg gctgcggtca tctgcggata
     5881 tggctttgcc tgggtggggc atttcttctt cgagaagaat cgcccggcta ctttccggca
     5941 tccgctctat agcctgatgg gcgattgggt gatgttcaag gacatctgcg tcgggaaaat
     6001 ttctctgtag gtccgccggc gcgttcaggc tgcctgctca tgcagcctga tgaggcattg
     6061 aagaggcgcc ttgcccattt tcctgggcac gcgttgcccg tgaggcagca cgcgccgcca
     6121 gcaacgcggc gagcgtcact ttcagtttgt cgttttccag cgccgccaga agcgtctcgc
     6181 catccacgcg cgtggcgtgc agttgaagct gatattcgag ctccgcgcga tcgatcacga
     6241 acaggtcgaa cagctgctca accgtgatct gcgccggatt cgccagcagc aggaagtgcg
     6301 gacgcaagcc gtcctcttgc aagcgcgcga tccactcgat ctcctcgagc ttttgcagca
     6361 gcttgatcgt ggtgtccatc tcgcgacgaa gcattcgcga gagttcctgc accgtgtagc
     6421 cgcgcgtgcc ggcgtcgcgc gcttccgaga gccgtgcgag caattcaagc gaatcgaaca
     6481 gatcgctgcc ctcgaaagtc ggccgatgaa actggccgat ccggatcgcc ggtaacgccg
     6541 acgcgatcat cgcgccggcc agtgcgatga accagcagag gtacatccac agcagaaaca
     6601 gcggcacggc ggcaaatgcg ccatacaccg ccgtgtaggt gggaatccgg cgcacgtaat
     6661 agccaaagcc gcgcttggcg agttcgaatg cgatcgcggc cgtaatcccg ccaatcaccg
     6721 cgtcgcgcca ttccacgcgg cagttcggca aaaacacgta aagcatcgtg aaggcgagcg
     6781 ccgtcaaggg caacgcggcg ctggtcagcg cccagtcgat caccggcgta atgtgctgag
     6841 cggcgctgaa cgccatggac cgcgtgaaca gataggaaga aatcgacaga ctcacgccga
     6901 tcagaatcgg accgagcgtg atgatcgccc agtagaccag aatgcgctgc gcgattggcc
     6961 gcgccttgcg tacccgccag atcacgttga aggcggactc cacggtcatc atcgtcatca
     7021 cggcggtgac gaacagaaaa atcatgccga tggtggtgag gcccttcgcc ttggacgcga
     7081 actggttcag ataattgaaa atctgactgt tgagctgcgc gggcatcaga tggtcggcga
     7141 ggaaaatctg cagcgacatc tgaaatgaac tgaaaatcgg aaacgcggtg aacagcgcaa
     7201 atgcgaccgt ggcaagcggc acgagcgcga gcatggtggt gaacgtgagg ctgccggcca
     7261 cctgcggaat gcgctcttcg ctgctgcgct tcgcggcaaa ctgcgcgagc cgtttgagcg
     7321 tgtcgagatc gaaacgcacc ctggacaaca aacccacctc cttgcttgat caaacagtgt
     7381 gtcaggacag agcgcgaggc ctggccgaat ggctatggat ggcctgtgca gcggacccag
     7441 cccctataat acccgctcac cgatgaggcc tatgaaagac attctcgtgc tttattacag
     7501 ccgtcacggc gccacgcgcg agcttgcgct ggcgatcgcc cacggcgtcg acagcgtccc
     7561 cggcatgcag gcgcgcgtgc gcaccgtgcc gccggtttcc accgtctgcg aagcgaccca
     7621 gccggatatt cccgctgaag ggccgcccta cgtcgaactg cgcgacctcg aggaatgcgc
     7681 cggtctcgcg ctcggctcgc ccacccgctt cggcaacatg gccgcatcgc tgaaatactt
     7741 tctcgacggc accacgccgc agtggcttgc gggtgcgctg tccggcaaac cggcctgcgt
     7801 gtttacgtcc accggcagtt tgcacggcgg ccaggaatcc acgctgttgt cgatgatgtt
     7861 gccgctcctg catcacggca tgctgatcgt cgggatcccg tacaccgaga gcgcgctgtc
     7921 caccacgcaa accggcggca cgccctacgg cgcgtcgcat ttcgcccgcg cgggcacagc
     7981 cggacagggc atctccgccg acgaaaaaac actggcgatt gccctcggcg cacgcgttgc
     8041 ccgcgcggcg gcatccctga gcgaacgcac gtgagcgcag acaacgccga acaagccggc
     8101 gcgtcagcgg cggacccgac cactgcggag cccgcccctc gaaatgccgc cgccgcattc
     8161 ggcgccagcg ctgcgctgat cgcgctgacc gggttgtcgg tcgcgtggga atggtggctc
     8221 gcgccgctgc gtccaggcgg ctcggcgctg gtgctcaaag ccgtgccgct tctgctcgcc
     8281 ctgcccggcg tgtggcggca tcggctttat acgttgcaat gggcgtcgat gctgatcctg
     8341 ctgtattttg ccgagggtat cgtccgcggc tggagcgatc gcggcctgag cgcccgcctc
     8401 ggttggctcg aagccgcact ttccattatt tttttcgtct gcacgctcgt ctatgtcggc
     8461 ccgttcaagc gcgccgcaaa acaggcagcc aaacaggcca cgcagcaaac aacccaacgg
     8521 gcaactgagc aggtcacaca acaagcatcg aaacaggcaa cacctgacaa caccaagccg
     8581 aacgccgagc aggccgaggg aggcgcccga tgaaaacagc gcatcccgcg ta
//
LOCUS       DATASH010000631         3504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1094105,
            whole genome shotgun sequence.
ACCESSION   DATASH010000631 DATASH010000000
VERSION     DATASH010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094105"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VHV99_17205"
     CDS             complement(<1..426)
                     /locus_tag="VHV99_17205"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEX3637186.1"
                     /translation="MIELHAFDDQRAQSDALAKAVGDALHASLAAQAAASGTGTSGTG
                     TSATGMSATGMSAAGTPNTGARPAMLAVSGGTSPRPFLQTLSTQSFDWARIAVTLVDD
                     RWVPETDGASNSQLVHDTLLQNAAKHAAFWPLVDTTQELN"
     gene            complement(630..2093)
                     /gene="zwf"
                     /locus_tag="VHV99_17210"
     CDS             complement(630..2093)
                     /gene="zwf"
                     /locus_tag="VHV99_17210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012213936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX3637187.1"
                     /translation="MQTDSSFTFVLFGGTGDLSMRKILPALYEAHRAGGMLAESGKIV
                     AVARHVADRAAYLDWVNEHVKPHVSKNGVDETAWASFLERIEFVKIDLGNPEDFTLLR
                     DALSGRPGIRVFYLATGPSLFVPICRALASVGLNENSRIVLEKPLGYDLKSSNAINDA
                     VGEIFAEEQIYRIDHYLGKEPVQNLLALRFGNVLFEPLWRREWVESIQITIAEELGVE
                     ARGDFYDNTGALRDMVQNHLLQLLSIVAMEPPHSMDSDSVRDEKLRVLRALKPIDPRD
                     ISKIAVRGQYHAGVIRGNAVPAYATEPGVKPDSTTETFVAVKVEIENWRWAGVPFFLR
                     TGKRLADRVAEIVVNFRAVPHSALGASALRGGANRLVIRLQPNETIRLYCLAKQPGEG
                     MNLASVHLDLAFDRFFREGQMEAYQRLLLDVINGRLALFVRRDEQEAAWRWVEPILNE
                     WAASNKPPKPYAAGTWGPAAASAMLAQHDTCWLEEEN"
     gene            2628..>3504
                     /locus_tag="VHV99_17215"
     CDS             2628..>3504
                     /locus_tag="VHV99_17215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3637188.1"
                     /translation="MKFRAIMGALCAAGLMCGVSAVQAAESVEVLHWWTSGGESKAVG
                     VLKDDMTKQGYTWKDFAVAGGAGAAAMTALKTQVISGNAPSAAQIKGPLIQDWASQGV
                     LVPIDSVAGDWKKNLPPEIDKIMHADGHYVAAPFSVHRVNWLYINKAALDKAGGKAPT
                     TWPEFFAVADKMKAAGIQPIAMGGQPWQDLTLWEDVVLSQGAGFYKKALVDLDEKTLT
                     SDQMVGVFDTVRKIQGYFDAGRTGRDWNLATAMVINGKAGMQFMGDWAKGEFANAGKK
                     SGTDYVCAAVPGTEKS"
BASE COUNT          669 a         1055 c         1114 g          666 t
ORIGIN      
        1 gttcaactct tgcgtggtgt cgaccagtgg ccagaaagcg gcgtgcttcg cggcattctg
       61 caacagcgtg tcgtgcacga gttgcgaatt gctcgcgccg tccgtttccg gcacccagcg
      121 gtcgtcgacc agcgtcacgg caatgcgcgc ccagtcgaag gattgcgtgg acaaggtctg
      181 caggaacggc cgcggactgg tgccgccgga tactgcaagc attgccgggc gcgcaccggt
      241 atttggcgtg cccgctgcgg acatgccggt tgcggacatg ccggtcgcgg acgtgccggt
      301 accggacgtg ccggtaccgg acgcagccgc ctgtgcggcg agcgacgcat gtaacgcgtc
      361 gccaaccgct ttcgccaacg cgtcggattg ggcgcgttga tcgtcgaaag cgtgaagctc
      421 gatcacttct cctccgtgct gccttcttgt taaatccgcg gaatgtgccg tcctgctctc
      481 acgcgatgcc tgcaagcggg atgacttgca ggcgccttcg tgataccggc gccattccgt
      541 tcagttcgtc ggtgggtctt acaggcgctg tgccgtgctt cgatcgggga tcgtgatcgg
      601 gcacggcacg gcgccgtggt tcagttgatt caattctctt cttcgagcca gcaggtgtca
      661 tgctgcgcca gcatcgcgct cgccgcggcg ggtccccacg tgcccgctgc atacggtttg
      721 ggcggcttgt tcgaggccgc ccattcgttg aggatcggct cgacccaacg ccatgccgct
      781 tcctgctcat cgcgccgcac aaacagcgcg aggcggccgt tgatgacgtc gagcaacaga
      841 cgctgatacg cctccatctg cccctcgcgg aagaaccggt cgaacgcgag gtcgaggtgg
      901 acgctggcaa ggttcatccc ttcgcccggc tgcttcgcga gacagtagag gcgaatggtt
      961 tcgttcggct gcaagcggat cacgagacgg ttcgcaccgc cgcgcagcgc ggatgcgccg
     1021 agtgccgaat gcggcaccgc acgaaaattc accacgatct cagccacgcg atccgcgagg
     1081 cgcttgccgg tgcgcaggaa gaacggcacg cccgcccagc gccaattttc gatttccacc
     1141 ttcacggcga cgaaggtttc ggtcgtgctg tccggcttca cgccaggctc ggttgcatac
     1201 gccggcaccg cgttgccgcg gatcacgccg gcatgatact ggccgcgcac cgcgatcttg
     1261 ctgatgtcgc gcggatcgat cggcttcaac gcgcgcagca cacgcagctt ttcgtcacgt
     1321 accgagtcgg aatccatcga atgcggcggc tccatcgcga cgatggaaag cagttgcagc
     1381 aaatggttct gcaccatatc gcgcaacgcg ccggtgttgt cgtagaagtc gccgcgcgct
     1441 tccacgccga gttcttccgc aatcgtgatc tgaatgcttt ccacccattc acggcgccac
     1501 aacggttcga acagcacatt gccgaaacgc agcgcgagca ggttctgcac cggttcctta
     1561 ccgaggtagt ggtcgatccg gtagatctgt tcttcagcga agatttcgcc aactgcatca
     1621 ttaattgcgt tggacgattt gaggtcgtag ccgagcggct tttccagcac gatgcgcgaa
     1681 ttctcgttga ggcccaccga agccagcgcg cggcagatcg gcacgaacag cgacggaccc
     1741 gtggccagat agaacacccg gatgccgggc cgcccgctca aggcgtcgcg cagcagcgtg
     1801 aagtcttcgg ggttgccgag atcgatcttc acgaactcga tacgttcgag gaagctcgcc
     1861 cacgctgttt catccacacc gttcttcgac acgtgcggct tgacgtgctc attgacccag
     1921 tcaaggtagg cagcccgatc ggccacatgc cgcgccaccg cgacgatctt gccactctca
     1981 gccagcatgc cgcctgcgcg gtgcgcctca tacagtgccg gcaggatctt gcgcatcgac
     2041 agatcgccgg tgccgccgaa gagcacgaag gtgaagcttg aatcggtttg catgagtctc
     2101 cgtcgatatc ctgagggccg ccgtggggac cgaaaaaggg cgaccgtagt ccgataaaat
     2161 attttttgac actgaattgt agtttaacta caatccaaat caagaggtag cgctaattcg
     2221 atgaaaaaag cgtgcgctgt gaggcaagat gcgcgttttc ccgaatcggc aggcgtcccc
     2281 tgcatgttaa cggacattgc gtgcgagcac gtgccagcat gccatcggcg gaccttacgg
     2341 aaagctggag ggctgagctg gtgtcagggt taacgtgtga ggcacctcga gtctgccgcg
     2401 cgcgacgcgg gcaggcaaaa atcaaaagag gagacagtca gttgcatccc gaaccactca
     2461 tctcttgagc ttccagcggc cggacatcgg tccgccggca catgcctcga cgcatgcgcc
     2521 cgaccgtgct cgatcgccca gtgttttgcc tgtttgaacc aaccacagca ccctggcgac
     2581 atcaggggcc atttgttttt tgttcaggtg tctggaggag ataagcaatg aaatttcgcg
     2641 cgatcatggg cgctttgtgc gccgcgggtc tgatgtgtgg cgtctcggct gtgcaagccg
     2701 ccgagtcggt cgaagtgttg cactggtgga cttcgggcgg tgaatcgaaa gccgtcggcg
     2761 tcctcaagga cgacatgacg aagcagggtt acacgtggaa ggacttcgcg gttgctggcg
     2821 gcgcaggtgc ggctgccatg acggcactca agacgcaggt gatctcgggc aacgcgccga
     2881 gcgctgcgca gatcaagggt ccgctgatcc aggactgggc ttcgcaaggc gtgctggtgc
     2941 cgatcgactc ggtcgctggc gactggaaaa agaacctgcc gccggaaatc gacaagatca
     3001 tgcacgcaga cggtcattac gtggcggcgc cgttctcggt gcaccgcgtg aactggctgt
     3061 acatcaacaa ggcagcactg gacaaggcag gcggcaaggc gccgaccacg tggcctgagt
     3121 tcttcgccgt ggcggacaag atgaaggccg cgggcatcca gccgatcgcg atgggcggtc
     3181 agccgtggca agacctgacg ctgtgggaag acgtcgtgct gtcgcaaggc gcgggcttct
     3241 acaagaaggc actggttgat ctcgacgaga agacgctgac atcggaccag atggtcggcg
     3301 tgttcgacac ggtccgcaag atccagggtt acttcgacgc gggccgcacg ggccgtgact
     3361 ggaacctggc aacggccatg gtcatcaacg gcaaggctgg tatgcagttc atgggcgact
     3421 gggcgaaggg cgaattcgcc aacgccggca agaagtcggg cacggactac gtctgcgctg
     3481 ctgtgccggg cacggaaaag tcct
//
LOCUS       DATASH010000632         4667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1094264,
            whole genome shotgun sequence.
ACCESSION   DATASH010000632 DATASH010000000
VERSION     DATASH010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094264"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VHV99_17220"
     CDS             <1..302
                     /locus_tag="VHV99_17220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637189.1"
                     /translation="RLRLGEMPNEPNEPNEPNEPNEPNEPNEPNEPNEPNEPNEPNEP
                     NEPNEPNEPNEPNEPNEPNEPNEPNEQVSANGARTRSAAIGFEKWASARAALPEV"
     gene            868..2136
                     /locus_tag="VHV99_17225"
     CDS             868..2136
                     /locus_tag="VHV99_17225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX3637190.1"
                     /translation="MTIAQHERTASAPHGFDVEVTSGLERFSAQHGELTLTSVFQPIF
                     SLSHMRAVGYEGLLRAHDALDRPVSPLDVFGEAARLGDVLQVDRLAQTLHLENFKVLG
                     AEREWLFLNVHPGALTDPYLAAALLANLRRLDLSPRRIVLEVLEQRAEDLERLAEAVR
                     QFRERGFLIALDDFGAGHSNVERIWQLNPDIVKLDRIMLSHAAHRADMATILPGLVAL
                     LHEAGKLVLIEGVETEHEAQMALACDADFVQGFFFGRPNPGAADAAHAATCISEVTDR
                     YRDQAEARERRNASRLTPYLRAFERAAERLAAGEPLEEVCWNFLALDHAARCFLLDAK
                     GKQAGRNVVLRADRAAHETRFLPLADAQGANWLRRPYFRAAINAPERVHVTRPYLSIN
                     EALPCVTLSVATRVGEQTCVLCGDIDWVEE"
     gene            2412..3203
                     /locus_tag="VHV99_17230"
     CDS             2412..3203
                     /locus_tag="VHV99_17230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEX3637191.1"
                     /translation="MSVLLEVIATTVADARLAAQAGANRLELVTAMGEGGLTPSLGLI
                     EAVVAAMDIRAAGAQAAGVLAVDVPAASVPAAGAPAAGAPVNVIVRPHSRSFVYDADD
                     YAVMLRDVRAVKAAGANGVVIGMLTADGEIDREGLARAIDAADGLVITFHRAFDETRD
                     LRKALDVLLGFDAVTNVLTSGGKPSVLHAESIMRELVRQAAGSHCTVLAGAGLTVDAV
                     AGFVSETQVEAVHFGSGVRVGGNGLAPVDAAKVAQVRALLDATHS"
     gene            complement(3369..>4667)
                     /locus_tag="VHV99_17235"
     CDS             complement(3369..>4667)
                     /locus_tag="VHV99_17235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004522968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6537 domain-containing protein"
                     /protein_id="HEX3637192.1"
                     /translation="LDFMGFAQKGGSVLSFVRFAARDEWLNQVRIDTQQADVLLACDM
                     VVGASADALQTVRHGRTRIVVNTHAIPNATFVQNPDATLHADALIDKMRHAAGVDRMS
                     TCDAQALATRFLGDTIGANILMLGYAWQLGLVPVSFGAMMRAIELNNVAVQMNQLAFS
                     IGRVAAEDPAALESLWQARHLARQPVRVDTLDELIAHREGRLQTYGGASYVKRYRALV
                     DAARRGEIAVDAKSERITRAVATTFYRLLAVKDEYEVARLHTDSAFREALEAQFEGVA
                     GKDFGIKFNLAPPTLTRPQPGVNPAKKTFGQWMWPVLGVMAKFSGLRGTMFDPFGRTL
                     ERKMERELADDYATTLQRAFAKLNANNLEDVAKLADLHARIRGYGHVKLANLAGVKRG
                     ERDLAARLQIEAATSASVKKSLEEVKGAGQLRGIPVVVAK"
BASE COUNT          895 a         1410 c         1505 g          857 t
ORIGIN      
        1 atcggcttcg cctcggcgaa atgccaaatg agccaaatga gccaaatgag ccaaatgagc
       61 caaatgagcc aaatgagcca aatgagccaa atgagccaaa tgagccaaat gagccaaatg
      121 agccaaatga gccgaatgag ccgaatgagc cgaatgagcc gaatgagccg aatgagccga
      181 atgagccgaa tgagccgaat gagccgaatg agcaggtaag cgcgaatgga gcgcgaacgc
      241 gaagcgccgc aatcggtttt gagaaatggg cttcggcgcg cgcagcgctg ccggaagtat
      301 gacggctgtg tcaccagggc tgaatgggcg agggcacgga ttccagatgc accactaccg
      361 tagctgtgcg ggggagccgc ttaagaatta gcggggtgag ccgctttctt ttgcctactt
      421 ttctttgcgg caggcaaaga aaagtaggtg cccgccccgc acaggggcga cgctaataga
      481 ccaataacaa atcaaggaaa ggccaacgcc gcagccgtac agagaccaag cgccgcgcag
      541 gcaaaaaaac caaaccttca gacgcccgtg atgcggtaag gccgaaattt atcccagcgg
      601 cgcgtcacgc cagcgtccag cgtccagcga tctcgggcag caagcgcccc ccataaggcg
      661 atgcaccgta acggtgcgca tcgcgcccac aggcggcgca tcgccccttt caccggtgca
      721 atctttgatg caaattccaa ccgaatgtgc gccaccgtgc gcatagagct tgcttaacat
      781 aggtcgatga cgcacatttt gagaaacggt gccgaggttg ttcgcctccg cgttcggacc
      841 ggcccggctc tcgcgtgaga ggattgcatg acgattgcgc aacacgaaag gacggccagc
      901 gcgccgcatg gcttcgatgt cgaggtgacg tcggggctcg agcgtttttc agcgcagcat
      961 ggcgagctga cgttgaccag cgtgtttcaa ccgattttca gcctgtcgca catgcgggcg
     1021 gtgggctacg aggggctgct gcgtgcgcac gatgcgctcg accgtcccgt gtcgccgctc
     1081 gacgtcttcg gcgaagcggc gcgccttggc gacgtgctgc aagtcgaccg gcttgcccag
     1141 actctgcatc ttgagaactt caaggtgctc ggcgccgagc gggaatggct gttcctgaac
     1201 gtccatcccg gcgcgctcac cgatccatat ctcgctgccg ccttgctggc caacctcaga
     1261 cggctcgatc tgtcgccgcg gcggatcgtg ctcgaggtgc tcgagcagcg cgcggaggat
     1321 ctcgaacgtc tcgccgaggc ggtacgccag ttccgtgagc gcggtttcct gattgcactc
     1381 gacgacttcg gcgcgggcca ttcgaatgtc gagcggatct ggcaattgaa tcccgatatc
     1441 gtgaagctcg accgcatcat gctgtcgcac gccgcgcatc gtgcggatat ggcgacgatc
     1501 ctgccggggc tggtggcgtt gctgcacgaa gcgggcaaac tggtgctgat cgaaggcgtg
     1561 gaaacagagc atgaagcgca gatggcgctc gcctgcgacg ctgacttcgt acagggcttt
     1621 ttcttcggcc gtcccaatcc cggcgcggcc gatgcggcgc acgccgcgac ttgcatcagc
     1681 gaagtcaccg accgttatcg cgatcaggcc gaggcgcgcg agcgccgcaa tgcgagccgt
     1741 ctcacaccgt acttgcgagc gttcgagcgc gccgcggagc ggcttgccgc cggtgaaccg
     1801 ctcgaggaag tgtgctggaa tttcctcgcg ctcgaccacg ctgcgcgctg cttcctgctc
     1861 gacgcgaagg gcaagcaggc gggtcgcaac gttgtattgc gcgccgatcg cgcggcgcac
     1921 gagacgcgct ttttgccgtt ggccgatgcg caaggggcga actggctgcg tcgtccgtat
     1981 ttccgcgcgg cgatcaatgc accggaacgc gtgcatgtga cgcgtccgta tctgtcgatc
     2041 aacgaggcgt tgccgtgcgt gacgctctcg gtggcgacgc gcgtcggcga gcagacgtgc
     2101 gtgctgtgcg gcgatatcga ttgggttgag gaatagcggc tctccgatgg ttctattggg
     2161 agagcggcgg ttctggtggt ccagatcgag aaggtgagcg gcggttctgg tagtccagat
     2221 cgagaaggtg agcggcggtt ctgtggtcca gatcgagaag gtgagcggcg gttctgtggt
     2281 ccagatcgag aaggcgagcg gcggtcttgt ggtccagatc gagaaggtga gcggcgtttt
     2341 gtggtccaga tcaagatggg cgaacagtgg ttctgagatc atgtcgtttt taacgacact
     2401 cagccacgcc tatgtccgtc ctgctcgaag tgatcgccac gaccgttgcc gacgcccggc
     2461 ttgcagcgca ggcgggtgcg aaccgtcttg aactcgtcac cgcgatgggc gaaggtggtc
     2521 tgacgccgag cctcgggttg atcgaagcgg tggttgccgc gatggatatc cgggcggctg
     2581 gcgcgcaggc ggctggcgtc ctggccgttg acgttccggc ggccagcgtc ccggcggccg
     2641 gcgccccggc ggccggcgct ccggtcaacg tcattgtgcg accgcatagc cgctcgttcg
     2701 tctacgacgc ggacgactac gccgtgatgc tgcgcgacgt gcgcgccgtc aaagcggccg
     2761 gtgcgaacgg cgtggtgatc ggcatgctga cggcagacgg tgagatcgac cgcgaaggcc
     2821 tcgcgcgtgc aatcgatgcg gcggatggtc tcgtcatcac cttccatcgt gcgttcgacg
     2881 aaacgcgtga tctacgaaaa gcgctcgacg tgctgctcgg tttcgatgcc gtgacgaatg
     2941 tcctgacctc gggcggaaaa ccatcggtac tgcacgcaga gtcgatcatg cgcgagctgg
     3001 tgcggcaagc ggccgggtca cattgcacgg tgctggcggg cgcgggtttg accgtcgatg
     3061 cggttgccgg cttcgtgagc gagacacagg tcgaagccgt gcacttcggt tcgggcgtgc
     3121 gagtcggcgg caacggcctc gcaccggtcg atgcggcaaa agtcgcgcag gtgcgagcgc
     3181 tactggacgc aacgcatagc tgattcatca ggaagacaat cagcgctatt ggacgcagag
     3241 cgtagcaaaa cgatccggca gaaaatcaac gctactagag gcaacgtgta acaaatccat
     3301 caggcaaaaa aatgccgctt cgtgaggttc aactcgaagc ggcattttcc atccacgcgc
     3361 gaaagaattt acttcgcgac gaccaccgga atcccacgca actgtccggc gcccttcacc
     3421 tcttccaacg actttttcac cgacgcgctc gtcgccgctt cgatctgcaa gcgcgcggcg
     3481 agatcacgct cgccgcgctt cacgcccgcc agatttgcca gtttcacgtg accgtagccg
     3541 cgtatacgag catgcagatc ggcgagcttc gcgacgtctt cgagattgtt cgcgttgagc
     3601 ttcgcaaaag cacgttgcag cgtggttgca tagtcgtcgg caagttcgcg ttccattttg
     3661 cgttccagcg tgcggccgaa cggatcgaac atcgtgccgc gcagaccgct gaatttcgcc
     3721 atcacgccga gcaccggcca catccattga ccgaacgtct tcttggccgg attcacgccc
     3781 ggttgtggac gcgtgagcgt cggcggcgcg aggttgaact tgatgccgaa gtccttgccc
     3841 gccacgcctt cgaattgcgc ctccagtgct tcacggaacg cgctatccgt atgcaggcgc
     3901 gccacttcgt attcgtcctt caccgcaagc agacgataga acgtcgtcgc gaccgcgcgc
     3961 gtgatgcgct cgctctttgc atcgaccgcg atttcgccgc gacgcgccgc atcgaccaac
     4021 gcgcgataac gcttcacgta gctcgcacca ccgtaggtct ggagacggcc ctcgcgatgc
     4081 gcgatcagtt cgtcgagcgt atcgacacgc accggctgcc tggccagatg ccgtgcttgc
     4141 cacagcgatt cgagcgcggc cggatcttca gcggccacgc ggccgattga aaacgccagc
     4201 tgattcatct gcaccgcgac gttgttcaac tcgatcgcgc gcatcatcgc gccgaacgag
     4261 actggcacga ggccgagttg ccaggcatag ccgagcatca gaatattcgc gccgatggtg
     4321 tcgccgagaa agcgcgtggc gagcgcctgg gcgtcgcacg tcgacatgcg gtcaacgccc
     4381 gccgcgtgac gcatcttgtc gattagcgcg tccgcatgca acgtcgcgtc cgggttctgc
     4441 acgaaggtcg cgttcggaat ggcgtgcgtg ttgacgacga tccgggtgcg gccatgacgt
     4501 actgtttgca gcgcatcggc gctcgcgccc accaccatgt cgcaggcgag cagcacgtcg
     4561 gcctgttgcg tgtcgatgcg cacctgattg agccactcgt cgcgcgcggc gaaacgcacg
     4621 aacgagagca ccgagccgcc cttttgcgcg aagcccatga agtcgag
//
LOCUS       DATASH010000633         7416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2081601,
            whole genome shotgun sequence.
ACCESSION   DATASH010000633 DATASH010000000
VERSION     DATASH010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7416
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2081601"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VHV99_17240"
     CDS             <1..201
                     /locus_tag="VHV99_17240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637193.1"
                     /translation="EPYFAGSVPFGGVWDVSFDNQRVDADSRGSGVHGRMTNMFRETF
                     HGTFQLRAERRSVHFHVIPCEF"
     gene            134..2128
                     /gene="prpR"
                     /locus_tag="VHV99_17245"
     CDS             134..2128
                     /gene="prpR"
                     /locus_tag="VHV99_17245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HEX3637194.1"
                     /translation="MERFNSGRSVAVSTFTSSLANSNSGRPGVALVSISRLQSLCEAV
                     APRYTGRARFFSVREGYGAAVTALQAYVDAGSVDVVLAAGSNGAYLRDNLNVPVVMVK
                     VNGFDVLSAITRTTTAWPGAQIGLVLHETISHELADLSAWLNVGLKQRAYRSIDEVRL
                     AVGNLAAEGCSVIIGPGMACDFAQQAGLESVFLYSLGAVEEAFERSVELARVSRQKES
                     KRVRLNTIVAHLRDGVAAFDDAGQLEAVNPAMLDLLGLDREQDVPAQLTRAVGPLLRE
                     TLEHDMPVEERIEQIGGRALIVNCVPIVEQGLRSGSVVTVQDTLVAQRIDRSLRTSQR
                     PKHLVARHQLDDLVGNSAALERVRRLARAGAAHDATVLLSGESGTGKELVAQGIHNAS
                     RRRGNPFVAFNCAALPEGLIESELFGHEEGAFTGARRGGKPGLFEIAHTGTIFLDEIG
                     EMPAALQSRLLRVLQEREVMKLGSGRATPIDVRVIAATHRDLHALVEQGVFRADLYFR
                     LNLLQIELPPLRERRDDVAPLARHLLNRSALQYGLSGASLERVLAFLAPLFEQYAWPG
                     NVRELENLLARAAIYLGDSGGEGWQDLQAVFPEFGRMQQAQALKVPVAVAHATADPAV
                     RPPPTREDALHALEQAGGNRAAASRALGIGRTTLWRLMKD"
     gene            2265..2828
                     /gene="ahpC"
                     /locus_tag="VHV99_17250"
     CDS             2265..2828
                     /gene="ahpC"
                     /locus_tag="VHV99_17250"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017329783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HEX3637195.1"
                     /translation="MPIINSQVKPFKAQAYHNGDFQTVTEESLKGKWSVFVFYPADFT
                     FVCPTELGDLADRYDEFKKLGVEIYSVSTDTHFTHKAWHDTSDTIQKIKYPMIADPTL
                     AISRNFDVLIEEEGLALRGTFVINPEGEIKLAEIHDNGIGRDAGELLRKVQAAQYIAA
                     HPGEVCPAKWTPGADTLTPSLDLIGKI"
     gene            2978..4570
                     /gene="ahpF"
                     /locus_tag="VHV99_17255"
     CDS             2978..4570
                     /gene="ahpF"
                     /locus_tag="VHV99_17255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018438160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEX3637196.1"
                     /translation="MLDASLKTQLKTYLEKVSRPIEIVASLDDSAKSQELLALLNDIA
                     TLSERVTVIERRDDAQRKPSFSIGEPGKETGIRFAGIPMGHEFTSLVLALLQVGGHPV
                     KLEDAVIEQIRSLDGDYQFETYFSLSCQNCPEVVQALNVMALINPRIRHVAIDGALFQ
                     NEVEARQIMAVPTMFMNGEVFGQGRSGVKEILAKLDTNAGARAAKELEKKPVFDTLIV
                     GGGPAGAAAAIYSARKGIATGVVAERFGGQVLDTLAIENFVSVTETEGPKFAMALEQH
                     VKSYEVDIMDVQRAEALIPGRINEVRLANGAVLKAKTIILATGARWREINVPGEREYR
                     NHGVAYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVREVTLFEFGAQLRADEVL
                     QRKLRSLANVTIVTQAQTTEITGDGKKVNGLVYKDLRSGETKSIELEGVFVQIGLVPN
                     TEWLKGTVELSKHGEIVVDARGATSVPGVFAAGDVTTVPFKQIVIAVGEGAKASLSAF
                     DHLIRNSDADEVVSKAEAEATV"
     gene            complement(4848..5261)
                     /locus_tag="VHV99_17260"
     CDS             complement(4848..5261)
                     /locus_tag="VHV99_17260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013691036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX3637197.1"
                     /translation="MRYTLFENQKDAAILVARILLMVLFVMFGWSKLTGFSGTVAYMT
                     SSGAPLPELSAVIAVVMEFVVGIALLVGFYTRPLALLLALYTLGTAIIAHHYWNMTGA
                     MQYASMINFYKNISIMGGLLLLCVTGAGRYSIDRH"
     gene            complement(5359..6084)
                     /locus_tag="VHV99_17265"
     CDS             complement(5359..6084)
                     /locus_tag="VHV99_17265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010089234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX3637198.1"
                     /translation="MLDIRHANQRGRAEHGWLSSRHTFSFANYHDPKQNGFSDLLVIN
                     DDRVAPAQGFGKHPHRDMEIFSYVLEGALEHKDTMGTGSVIVPGDIQLMSAGTGVAHS
                     EYNHSKSDPVHFMQIWIAPAQKGTAPRYQQRHFGADQKRGVLRLVMSPDGAQDSLVLQ
                     QDARVYAGLFDGDETARLELASNRFAYIHVARGSVTVNGVELGEGDGARVRGEEALTF
                     TQGHDAEVLVFDLRNIEMSELWA"
     gene            6221..7099
                     /locus_tag="VHV99_17270"
     CDS             6221..7099
                     /locus_tag="VHV99_17270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009771195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3637199.1"
                     /translation="MQIDDVRIFVATVDARSFTAAAKRLALSKQFVSRRVMALEEALG
                     VQLLIRNTRKLAVTELGQEFYERAKRILSEVEDAEQAMSLRRAGPRGLLRVSAPMSFG
                     MMHLSPLVANFLREHGDVRFDMELSDRTVDVVGEGFDMAIRIGTLPDSTLIAQKLVDV
                     RMVACCSPGYVRRRGAPRHPAELARHSCLLYGHGGVVSWDFVVDGVSKGFEVEGPLRA
                     NNGELIRDAAVAGLGIVRLPDFIVAEALGGGQLVTVLEEFLPMAVTVYAVYPQHRQSS
                     VAVRAFVEFLRGRLMV"
BASE COUNT         1440 a         2112 c         2357 g         1507 t
ORIGIN      
        1 gagccgtatt ttgcaggaag cgtgccattc ggcggcgttt gggatgtttc ctttgataat
       61 cagcgggttg acgcggattc acggggctcg ggcgttcatg gcagaatgac gaacatgttc
      121 cgtgaaacgt ttcatggaac gtttcaactc cgggcggagc gtcgcagtgt ccactttcac
      181 gtcatccctt gcgaattcta attctggccg cccaggcgtc gccctggtca gtatcagccg
      241 attgcaatcg ctttgcgagg cggtcgcgcc gcgttatacc gggcgggcca ggttcttttc
      301 agtccgcgag ggctatggcg ccgccgtcac ggccctgcag gcctatgtcg acgccggctc
      361 ggtcgatgtc gtgctggcag ccggctcgaa cggtgcctac ctgcgcgaca atctgaacgt
      421 gccggtggtc atggtcaagg tgaacggctt cgacgtgctg agcgcgatca cccgcaccac
      481 gacggcctgg cctggcgcgc agatcggcct ggtcctgcac gaaaccatct cgcacgaact
      541 cgccgacctg agcgcttggc tgaatgtcgg cctgaaacag cgcgcctacc gctcgatcga
      601 cgaagtgcgg ctcgccgtcg gcaacctcgc ggccgaaggc tgcagcgtga tcatcggccc
      661 cggcatggcc tgcgatttcg cccagcaggc cggtctggag agtgtctttc tgtactcgct
      721 gggcgccgtc gaagaagcat tcgagcgctc ggtcgagctg gcgcgcgtga gccgccagaa
      781 ggaatcgaag cgggttcgcc tgaatacgat cgttgcccat ctgcgcgatg gcgtggccgc
      841 ttttgacgac gccggccaac tcgaggcagt caatccggcg atgctcgatc tgctcggcct
      901 cgatcgcgaa caggacgtcc cggcgcaatt gacgcgtgcc gtcggcccgt tgttgcgcga
      961 gacgctcgag catgacatgc cggtcgaaga gcgtatcgag cagattggcg gccgcgcgct
     1021 gatcgtcaac tgtgtgccga tcgtcgagca gggtctgcgt tcgggctccg ttgtcaccgt
     1081 gcaggacacg ctggtcgcgc agcggatcga ccgctcgctg cgtaccagcc agcggccgaa
     1141 gcatctggtc gcgcggcacc agctggacga tctggtgggc aattcggcgg cgctcgagcg
     1201 ggtgcggcgg ctcgcgcgtg ccggcgcggc tcacgatgcc accgtgctgt tgagcggcga
     1261 aagcggcacc ggcaaggaac tggtcgcgca gggcatccac aatgccagcc ggcgccgcgg
     1321 taatccgttt gtcgccttca attgcgcggc gttgcccgaa ggcctgatcg aaagcgaatt
     1381 gttcggtcac gaggaaggtg cgttcaccgg cgcgcgccgg ggcggcaagc ctggcctctt
     1441 cgagattgcc cacaccggga cgatctttct cgatgaaatc ggcgagatgc cggcggcgct
     1501 gcaaagccgg ctgctgcggg tattgcagga gcgcgaagtc atgaagctcg gctccgggcg
     1561 cgcaaccccc atcgacgtgc gggtgatcgc cgccacgcat cgcgatctgc acgcgctggt
     1621 cgaacagggg gtgtttcgcg cggatctgta ttttcgtttg aacctgcttc agatagaatt
     1681 gccgccgcta cgcgaaagac gtgacgatgt cgcgccgctt gcgcggcacc tgttgaaccg
     1741 cagcgcgctc caatacgggc tgtcgggtgc atcgctagag cgggtgctgg cgttcctcgc
     1801 gccgttgttc gagcagtatg cgtggccggg caatgtgagg gagctggaaa atctgctggc
     1861 gcgtgccgcg atttatctgg gcgattcggg cggggaaggc tggcaggatc tgcaggcggt
     1921 ctttccggaa tttggccgga tgcagcaggc gcaggctttg aaagtgccag ttgcagtggc
     1981 ccatgccacc gctgacccag cagttcgccc gccacccacc cgcgaggacg cgctacacgc
     2041 gctcgaacag gccggcggca atcgcgctgc agcaagccgg gcactgggaa tcggccgcac
     2101 gaccctctgg cggctcatga aggactgatg cggtgcaata gtcactggtc agacaataaa
     2161 tagtgtcagg ctatcaacac cattaaaaca ctccgcttta cctattaagc gctgtctgac
     2221 atactgactc cactttccaa ccactttgaa agaggactca gtaaatgccg atcatcaata
     2281 gtcaagtcaa accgttcaag gcacaggctt atcacaatgg cgattttcag accgtcactg
     2341 aagaaagcct gaagggcaag tggtcggttt tcgttttcta cccggctgac ttcacgtttg
     2401 tttgcccgac cgaactgggt gacctggccg atcgttacga cgaattcaag aagctcggcg
     2461 tcgaaatcta cagcgtgtcg accgatacgc acttcacgca caaggcatgg cacgacacgt
     2521 cggatacgat ccagaagatc aagtacccga tgatcgctga cccgaccctg gcaatctcgc
     2581 gcaacttcga cgtgctgatc gaagaagaag gtctggcact gcgcggcacg ttcgtgatca
     2641 atccggaagg cgagatcaag ctggccgaaa ttcacgacaa cggcattggc cgtgacgcag
     2701 gtgaattgct gcgcaaggtg caagctgcac aatacatcgc ggctcacccg ggtgaagttt
     2761 gccccgccaa gtggacgccg ggcgctgaca cgctgacccc gtcgctcgac ctgatcggca
     2821 agatctaagg tccaaaagcc tctagcagta ggcgcctcac ggcgcaccgc cgtgcggacc
     2881 gcttttcaca gcggttcgcg cgtccccggg ctgcgcgcct cctatctggt gcgtgcggcg
     2941 accgggaccc aataccctcg ccacggaatc gaaagccatg ctggacgcca gtctcaagac
     3001 tcagttgaaa acctacctcg aaaaggttag caggcctatc gagatcgtcg cctcgctcga
     3061 cgacagcgcg aaatcccagg aactgctggc attgctgaac gatatcgcga cgttatcgga
     3121 acgcgtcact gtgatcgaac gccgtgatga cgcgcaacgc aaaccgtcgt tctcgatcgg
     3181 cgagccgggc aaggaaacgg gcatccgttt cgccggcatt ccgatggggc atgaattcac
     3241 gtcgctcgtc ctcgcgcttt tgcaggtcgg tggccatccg gtcaaactcg aagatgcggt
     3301 gatcgaacag atccgcagtc tcgacggcga ctatcaattc gaaacgtatt tttcgctgtc
     3361 atgccagaac tgcccggaag tcgtccaggc actgaacgtg atggcgctga tcaacccgcg
     3421 catccgccac gtggcgatcg acggcgcgtt gttccagaac gaagtcgaag cgcgccagat
     3481 catggcggta ccgaccatgt tcatgaatgg cgaagtgttc ggccagggcc gcagcggcgt
     3541 gaaggaaatc ctcgcgaagc tcgacaccaa tgccggtgcc cgtgcggcca aggaactgga
     3601 aaagaagccg gtgttcgata cgctgatcgt cggcggcgga cctgcgggcg cggctgcggc
     3661 gatttactcg gcgcgcaagg ggattgccac gggtgtcgtg gcggaacgct tcggcggcca
     3721 ggtgctcgac acactggcga tcgagaactt tgtctccgtg acggaaaccg aaggaccaaa
     3781 gttcgccatg gcgctcgaac agcacgtaaa gagctacgaa gtcgacatca tggacgtaca
     3841 gcgcgctgaa gcgctgatcc cgggccgcat caacgaagtg cgtctggcaa acggcgcggt
     3901 gctcaaggcc aagacgatca ttctcgccac gggtgcccgc tggcgcgaaa tcaacgtgcc
     3961 gggcgagcgc gaataccgta accacggcgt cgcgtactgc ccgcattgcg atggtccgct
     4021 gttcaagggt aagcgcgttg cggtgatcgg cggcggcaac tcgggcgtgg aagcggcaat
     4081 cgacctcgcg ggtatcgtgc gtgaagtgac gctgttcgaa ttcggcgcac aactgcgtgc
     4141 tgacgaagtg ctgcaacgta agctgcgcag cctcgccaat gtgacgatcg tcacgcaggc
     4201 acaaaccacg gaaatcaccg gcgacggcaa gaaggtcaac ggtctggtct acaaggacct
     4261 gcgttcgggc gaaacgaaga gcatcgagct tgagggcgtg ttcgtgcaga tcggtctcgt
     4321 gccgaacacc gaatggttga agggcacggt cgagttgtcg aagcacggcg agatcgttgt
     4381 cgatgcacgc ggcgcaacct cggtgccggg cgtgttcgcc gccggcgacg tgaccacggt
     4441 gccgttcaaa cagatcgtga ttgcagtggg tgagggcgcg aaggcatcgc tctccgcgtt
     4501 tgatcacctg atccgcaata gcgatgcgga cgaagtggtg agcaaagcag aggctgaagc
     4561 gacagtttga tggttgtgtt tgagtagtgg acggcggcct tcgggccgcc gtttttgtgg
     4621 tcgcgcatta tcaaggcgat atggcgcagt cgaatatccg gacggcaatt cgcgagcgcg
     4681 tgttggcgca attcgccggc tggtacggat tgcttcatgc aaagaaaaat ccgcctgacg
     4741 actgcctgcg gattttttgt gagcgcccac gcaatccggt agtactggtg ctgatggacc
     4801 aaccggccca ctgcgttcgc gggcgtatcg acccctgcag tagagcctca atgccggtcg
     4861 atcgagtacc tgccggcgcc ggtcacgcac aataacaaca gtccacccat gatgctgatg
     4921 ttcttgtaga agttgatcat gctcgcgtat tgcatcgcac cagtcatgtt ccagtagtgg
     4981 tgggcaatga tcgccgtacc cagcgtatag agtgccagca gcagcgcgag cggtcgcgta
     5041 tagaagccca ccagcagcgc aatgcccacc acaaactcca tcacgacagc tatgaccgcc
     5101 gacaactcag gcagcggggc gccgctcgac gtcatataag cgacggtgcc ggaaaagccg
     5161 gtcagtttcg accagccgaa catcacgaac aggaccatca acaggatgcg ggcgacgaga
     5221 atggctgcgt ctttctggtt ttcaaatagc gtatagcgca tgatttttct ctgtaggaca
     5281 gcaagggtgc ggctcgccgc aaaacttcgc ggtgagccgc acttgaaccg gccagatcga
     5341 taggcaggat caagccgctt aagcccacaa ctccgacatt tcgatgttac gcaaatcgaa
     5401 caccaacacc tccgcgtcgt gaccttgcgt gaaggtcagg gcttcttcgc ctcgaacccg
     5461 tgcaccgtcg ccttcgccaa gttcgacacc gttcaccgtg acgctgccgc gcgccacatg
     5521 aatataagca aagcgattgc tggccagctc gaggcgcgca gtttcgtcac cgtcgaaaag
     5581 accggcatag acccgcgcat cctgttgcag taccagcgag tcttgcgcgc cgtccggtga
     5641 catcacgagg cgcaatacgc cgcgcttctg atccgcgcca aagtggcgtt gctgataacg
     5701 cggcgcagtg cctttctgcg ccggcgcaat ccagatttgc atgaagtgca ccgggtcgct
     5761 cttcgaatgg ttgtattcgc tgtgcgcgac gccggttccc gcgctcatca actggatgtc
     5821 gccaggcacg atcaccgagc cggtgcccat tgtgtcctta tgctcgagcg cgccttccag
     5881 cacgtacgaa aagatctcca tgtcacggtg cgggtgcttg ccgaaacctt gcgccggtgc
     5941 gacacggtcg tcgttgatca cgagcaggtc ggaaaagccg ttctgcttcg gatcgtgata
     6001 gttcgcgaag gaaaacgtat gacgcgaact gagccagccg tgctccgcgc ggccgcgttg
     6061 attggcgtgt ctgatatcga gcatcgtatt tctccttggg ttctgttgtg cgatccggcc
     6121 agaggtttgt ccgcgatcca tgaacgacag tgtaggtcaa tccggatgcg tataatcttt
     6181 gggtaaattg aaacactgtc tcgtcagagt ggacaatacg atgcaaatcg atgatgtacg
     6241 aatctttgtc gccacggtcg acgcccgtag cttcaccgct gcggcgaagc ggctggcgct
     6301 gtcgaagcag ttcgtcagcc ggcgcgtgat ggcgctcgaa gaggcgctcg gcgtgcaact
     6361 gctgatccgc aatacgcgca agctggccgt gaccgaactc ggtcaggagt tttacgagcg
     6421 tgccaaacga atcctcagcg aagtggaaga cgccgagcag gcaatgtcgt tgcgtcgcgc
     6481 cgggccgcgt gggctgttgc gggtcagtgc gccgatgtcg ttcgggatga tgcatctatc
     6541 gccgttggtg gcgaactttt tgcgcgagca tggcgatgtg cgcttcgata tggagttgag
     6601 cgatcgcacc gtcgacgtgg tgggcgaggg gttcgatatg gcgatccgga ttggcacgtt
     6661 gccggattcc acgctgatcg cgcagaaact tgtcgatgtc agaatggtgg cgtgttgtag
     6721 tccggggtat gtgcggcggc gtggagcgcc tcgtcatcct gctgaactgg cgcggcattc
     6781 gtgtctgttg tatgggcatg gtggggtggt gagttgggat tttgttgttg atggtgtttc
     6841 gaaagggttt gaggtggagg ggccgctgcg ggctaataat ggggagttga tccgcgacgc
     6901 ggcagtggca gggttgggga ttgttcggct tcctgatttt attgttgctg aagcgcttgg
     6961 gggtggtcag cttgtgactg tgttggagga gtttttgccg atggcggtta cggtttatgc
     7021 ggtttatccg cagcatcggc agagttctgt cgcggttagg gcttttgtgg agtttttgag
     7081 ggggaggttg atggtttgat tttttttgcc tgtgcggcgc ttgtggtctg tgcttttacg
     7141 gcgttggcct tttcttgaat tgatattggt ctattagcgt tgcccctgtg cggggcggca
     7201 cctacttctc tttgccggct gcaaagagaa gtaggcaaga gaaagcagct caaaccgcaa
     7261 attcctaagc gggtcccccg cgcagtcgcg gtagtggtgc atctggaatc cgtgccctcg
     7321 cacattcgac gcctgtgaca acgcgctcat cagctcccac tccgcactac gtgcgtcgcg
     7381 gatgggtcta cctggtaaac caggggcttc gtttgg
//
LOCUS       DATASH010000634         1580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4059150,
            whole genome shotgun sequence.
ACCESSION   DATASH010000634 DATASH010000000
VERSION     DATASH010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4059150"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1281)
                     /locus_tag="VHV99_17275"
     CDS             complement(<1..1281)
                     /locus_tag="VHV99_17275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014973051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX3637200.1"
                     /translation="MCIVNVALKRPYTFIVMAILILLATPFVLFTTPVDVLPEINIPV
                     VSIIWSYTGLSAEDMANRIASGNERGLTTTVNNIEHIESQSLAGISILKVFLQPDANI
                     QTAIAQVVAVEQAQLKQAPPGATPPLVISYSASSIPVIQLGLSSPKLSEQALNDTALN
                     FLRPQLVTIPGAAVPYPYGGKARLISVDLDTRALLAKGLTPSDVVNAFNAQNLILPTG
                     TAKIGPKEYTINMNGSPPTLDGLNDIPVRTLNGATTYLREVAHVRDGFSPQTNIVRQD
                     GRRGVLMSVLKNGNASTLAIVDRLKDLLPGARAALPPDLHITALFDQSVFVKAAVQGV
                     VREALVAAVLTAAMILLFLGNWRSTCIIAISIPLSILSSLIALHALGQTINIMTLGGL
                     ALAVGILVDDATVTIENIERHLHMGTNLHDAILEG"
BASE COUNT          334 a          446 c          488 g          312 t
ORIGIN      
        1 accttcgaga atcgcatcgt gcagattcgt gcccatatgc agatgccgtt cgatgttctc
       61 gatcgtcacc gtcgcatcgt cgaccagaat cccgaccgcc agcgcaaggc cgccgagcgt
      121 catgatgttg atggtttgcc cgagcgcgtg cagcgcgatc agcgaggaca gaatcgacag
      181 cggaatcgag atcgcgatga tgcaggtgct gcgccaattg ccgaggaaca gcagaatcat
      241 cgcggcggtc agcacggcgg cgaccagtgc ttcacgcacc acgccctgca ccgccgcttt
      301 cacgaacacc gactgatcga acagcgcggt aatgtgcagg tcgggcggca acgcagcgcg
      361 cgcgcccggc agcaggtcct tcagcctgtc cacgatcgcc agcgtcgagg cattgccgtt
      421 cttcagcact gacatcagca cgccgcgccg gccgtcctgc cgcacgatat tggtctgcgg
      481 cgagaagccg tcgcgcacat gggcaacctc gcgcagatac gtcgtcgcgc cgttgagcgt
      541 acgcaccggg atgtcgttga gcccgtccag cgtgggaggc gagccgttca tattgatcgt
      601 gtattccttc ggcccgatct tggccgtgcc ggtcggcaaa atcaggtttt gcgcattgaa
      661 cgcattgacc acgtccgaag gcgtcagccc ttttgccagc aacgcgcgcg tgtcgagatc
      721 gaccgagatc agacgcgctt tgccgccgta tggatacggc acggctgcac ccggaatcgt
      781 cacgagttgc ggacgcagga aattcagcgc ggtgtcgttg agcgcctgct ccgacagttt
      841 cggactcgac aaaccgagct ggattaccgg gatgctggag gccgagtagc tgatcaccaa
      901 cggcggtgtc gcgcctggtg gcgcctgttt gagctgcgcc tgttcgacgg ccaccacctg
      961 cgcgatcgcg gtctggatgt tcgcgtccgg ctgcaggaac accttgagaa tcgagatgcc
     1021 ggcaagcgac tgcgattcaa tgtgctcgat gttgttgacc gtggtggtca ggccgcgttc
     1081 gttgcccgac gcaatacggt tggccatgtc ttcggccgac aagcccgtgt aactccagat
     1141 gatgctgacc accggaatat tgatttccgg cagcacgtcg accggcgtgg tgaacagcac
     1201 gaatggcgtc gccagcaaga tcagaatggc catcacgatg aacgtgtatg gccgtttaag
     1261 cgcaacgttg acgatacaca tgaaacgcgc ccgaatgaat gcaggtaagg gatgggtgaa
     1321 tctgcgccta tgctagatcg gtggcaccaa cacctgcatg gcgcgaaaat ggcagaaatg
     1381 tcaggaagct aaatgaaagc ggcgatgcta atcggacgtc ggtggttttg gcgataagca
     1441 aacgtttgtt gaatcaagaa taagcgctgt tcgatgcacg ttggtccgcg tgaagtgtcg
     1501 cggcgatgcg aagtgttgtg gaatattcgt tcagattgac ttgtgcaaag caaatacttc
     1561 accgcttgaa tgaatgaaga
//
LOCUS       DATASH010000635         2199 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_895858,
            whole genome shotgun sequence.
ACCESSION   DATASH010000635 DATASH010000000
VERSION     DATASH010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_895858"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="VHV99_17280"
     CDS             complement(<1..427)
                     /locus_tag="VHV99_17280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX3637201.1"
                     /translation="MATSIAEHHAVRLAHGEAHAALHGLALTPLRRQVYAAIVASDRP
                     IGAYELLDALEPQRGRVPPTTVYRALDFLLAHGFVHRIESKNAFVACSEVGVPHSSQF
                     LMCDGCGATVEIPGGELARQLSHSAPAHGFEVHRQVVDLS"
     gene            738..1520
                     /locus_tag="VHV99_17285"
     CDS             738..1520
                     /locus_tag="VHV99_17285"
                     /EC_number="1.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-iditol 2-dehydrogenase"
                     /protein_id="HEX3637202.1"
                     /translation="MAARLQDKVAILTGAASGIGEAVARRYLDEGARCVLVDVKPANS
                     FGASLLSAYGDRVLTVSADVTRREDIERILASTLERFGQVDILFNNAALFDMRPILDE
                     SWDVFDRLFAVNVKGMFFLMQTVARKMVEQGRGGKIINMSSQAGRRGEALVSHYCATK
                     AAVLSYTQSAALALAPHQINVNGIAPGVVDTPMWNEVDALFARYENRPLGEKKRLVGE
                     AVPLGRMGVPEDLTGAALFLASADADYITAQTLNVDGGNWMS"
     gene            1618..>2199
                     /locus_tag="VHV99_17290"
     CDS             1618..>2199
                     /locus_tag="VHV99_17290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX3637203.1"
                     /translation="MKPALKSALKAVSAGAVACFALQAQAVTVTIATLNNPDMIELKK
                     LSPAFEKANPDIKLNWVILEENVLRQRATTDITTGSGQFDVMTIGAYETPQWGKRGWL
                     TPITNLPADYDLNDVVKTARDGLSSGGQLYALPFYVESSMTYYRKDLFAAKGMKMPDQ
                     PTYDQIAQFADKLTDKANGMYGICLRGKAGWGEN"
BASE COUNT          463 a          683 c          677 g          376 t
ORIGIN      
        1 cgctgagatc gaccacctgg cggtgcacct cgaaaccatg cgctggtgcg ctatgcgaca
       61 gctgccgggc aagctcgccg ccgggaatct cgacggtcgc gccgcagccg tcgcacatca
      121 gaaactggct gctgtgcggc acgccgactt cgctgcaggc cacgaacgcg ttcttcgatt
      181 cgatccgatg aacgaagcca tgcgcgagca gaaaatccag cgcccggtag acggtggtgg
      241 gcggcacgcg gccgcgttgc ggctcgagcg catccagcaa ctcataggcg ccgatcggcc
      301 ggtcactggc gacaatcgcc gcatacacct gacgccgcag cggcgtgagc gcgagtccat
      361 gcagggcggc atgcgcctcc ccatgggcga gccgtacggc atggtgttct gcaatcgatg
      421 tagccatcag cggacctcct gacgaactgg caaagcttca aagcgaagcg aggccgaaat
      481 gatataacgt atctcgaatt tttgtcagcg acttgcacag aaagcagctt ggcccggttc
      541 gatttctcgt attagtgttg acccgaagcc gccgccgctg cgatcgggtc ctgccgcgct
      601 gcaccatcga aatctggccg agcgccttga catgacccgg tccgtatccg atcattcgat
      661 cacaaacaga gcaattgttc gcgcaatgag cgttcgctca ctgcgcctca agaagcgaaa
      721 cgaaccggag acgagcagtg gcggcacgat tgcaagacaa ggtggccatt ctgacgggcg
      781 cggcaagcgg aatcggtgaa gccgtggccc ggcgctatct ggacgaaggc gcgcgctgcg
      841 tgctggtcga tgtgaagccg gcgaacagtt tcggcgcttc gttgctcagc gcttacggcg
      901 accgcgtgct gaccgtcagc gcagacgtga cgcgccgcga ggatatcgaa cgtattcttg
      961 ccagcacgct ggagcgcttc ggccaggtcg acattctgtt caacaacgcg gcgctcttcg
     1021 atatgcgccc gattctcgat gaatcctggg acgtgttcga ccgcctcttc gcggtcaatg
     1081 tgaagggcat gttcttcctg atgcaaaccg tcgcccgcaa aatggtcgag cagggccgcg
     1141 gcggcaagat catcaatatg tcgtcgcaag ccgggcggcg cggcgaggcg ctggtgtcgc
     1201 attactgcgc aaccaaggcc gccgtactca gctatacgca atccgccgcg ctcgcgctcg
     1261 cgccgcacca gatcaacgtg aacggcatcg cgccgggtgt cgtcgatacg ccgatgtgga
     1321 acgaggtcga cgcgctcttc gcccgttatg aaaaccgtcc gctcggcgag aagaagcgcc
     1381 tggtgggtga agcggtgccg cttggccgca tgggtgtgcc ggaagatctg accggcgctg
     1441 ccctgttcct cgcgtcggcg gatgccgact acatcaccgc gcaaaccctg aacgtggacg
     1501 gcgggaactg gatgagctga gtggatcaca gcgtggatga gccgtacccg cgttacccat
     1561 cgtcattttc gtagaacaaa cgcagcacca cataacgtac gagaaaggag acaacacatg
     1621 aaaccagccc tcaaatccgc actcaaggcg gtcagtgccg gcgcagtcgc atgcttcgcg
     1681 ctgcaggcgc aggcggtcac ggtgaccatc gccacgctga acaacccgga catgatcgag
     1741 ctgaagaagc tctcgcccgc tttcgagaag gcgaatccgg acatcaagct caactgggtg
     1801 attctcgaag aaaacgtgct gcgtcaacgc gctaccaccg acatcactac cggcagcggc
     1861 cagttcgacg tgatgacgat cggtgcctac gaaacgccgc agtggggcaa gcgtggctgg
     1921 ctcacgccga tcaccaatct gcccgccgat tacgatctga acgacgtcgt gaagacggcc
     1981 cgcgacggtc tctcgagcgg cggccagttg tatgcgctgc cgttctacgt cgaaagctcg
     2041 atgacgtatt accgcaaaga cctgttcgcg gcgaagggca tgaagatgcc ggatcagccc
     2101 acctacgacc agatcgcgca gttcgccgac aagctcaccg acaaagccaa cggcatgtac
     2161 ggcatttgtc tgcgcggcaa ggccggctgg ggcgaaaac
//
LOCUS       DATASH010000636         1824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6432448,
            whole genome shotgun sequence.
ACCESSION   DATASH010000636 DATASH010000000
VERSION     DATASH010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6432448"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VHV99_17295"
     CDS             <1..181
                     /locus_tag="VHV99_17295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637204.1"
                     /translation="MFQFIFMAYVLPVLRDASVAPSAITVIGFCVTVALAAASFHLFE
                     KPARALIRRRLSSGA"
     gene            complement(178..432)
                     /locus_tag="VHV99_17300"
     CDS             complement(178..432)
                     /locus_tag="VHV99_17300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637205.1"
                     /translation="MTQTSNTAIAVIGIDIAKNSFHVGHDARGAIVLRQGETTNDLFG
                     MPTSCERRSTRSAPCLRPSLPGPSPQPHKSTRLRAANAAG"
BASE COUNT          344 a          520 c          552 g          408 t
ORIGIN      
        1 tatgtttcag ttcattttta tggcctacgt gctgccggtc ctacgtgacg catcggtcgc
       61 cccctccgcg ataaccgtca ttggcttttg cgtaacggtg gccctcgcgg cggccagctt
      121 tcacttgttc gaaaagccgg cgcgcgcgct gatccggcgc cggctttcgt ccggcgccta
      181 gccggccgca ttggcagcac gcagccgcgt cgatttatgc ggttgtggtg atggtcccgg
      241 cagtgatggc cgcaggcacg gtgccgagcg tgtcgaacga cgctcgcacg aagtcggcat
      301 gccaaacagg tcattggtcg tctcgccttg ccgcagcacg atggcgccgc gcgcatcgtg
      361 gcccacgtgg aacgagttct tggcgatatc gatgccgatc acggcgatcg cggtattgga
      421 tgtctgagtc atggcgtgct cctcgtcttt cgcgccccgt gtcagcttca tttgctggcg
      481 gggtaggagc acggccggac catcccatta gcaggtcact gaaaaacctg aaagttgagg
      541 cgtgtctctc tgtcgagggg ccgtacgtgc ggtgatgtgt tcgcttgttc gaggcgcttt
      601 tggtggtccg aacagccgct cgactatgcc gccaccagct ggggaatgcg gatcaggtta
      661 agacgatcag attgagcatg aagtcggcgg cgacgcggca gatgccgcga tgtttggtct
      721 tgcgcatcgt gccgtgctgc ttgccccagc cgaagatgca ttcgatcatc gcgcggcgcg
      781 attgccatag ccgtcgtggc gcgtggtgcg ttcgtcgacg gcgctgcagc ggcgtttgcc
      841 ggttttgccg acaccgttgt tctgcgtcac atgcggtgtg acattgatgg cgcgattaag
      901 gtacgttgaa tccgtagcta tccactgtgc cgtgttcact tgggctgacc gaaacttgcg
      961 tgaatttaca atccgcttcc gagccaagtg tcatggcgat gcacatgcgc ccatgtcagt
     1021 cggccaacca tcccgcgtca caatgcttgt tcgttctgac acaagcgctg cgcgatcggt
     1081 ccggccgctc ggcgcttgtg ccaatattgc ggcaccaaat atccaaaaaa aaatcaaaat
     1141 cgcatcaaaa agtcgccaga ccgtcctctc cgcgagaatt gcaaggagca taggcgctga
     1201 ttcaggacgc atcgatgtcg tccaatgagg cgccagaaat gatcgcgctt cagaaattgc
     1261 atgcgcgtat ttcgaaaaga ctcagggaac acgcgacctc ggctggtact atcccgcctt
     1321 gtcccgttca attgcgcggg cagtgacaga acgcgcttgc acctgcgatg tatccctact
     1381 gattccgcat ggatgcactc gcatctgcga gcgcacaaca gcgcaagctg aatggcgcgg
     1441 cctgtctttt cggaagactt ttggcagtca gtggcgcatt tgagttcctg ttgactgggc
     1501 ccatgaaata ttcgcttgcc cacgacgaaa tgcggtgact gcgcacaccg gggtctgtgc
     1561 gggggcgagg agcaatcctg gtccctaccg cgacctcagt tggtgttctc atgcgtcatg
     1621 atagaaatca gtgcgctaca tgcgatgcaa gagcgcttcg atatcgggca aacagatcgt
     1681 tggatatacg atgtcggaat tcggactagg actccagaag atcgggtgcc ggcggcaatg
     1741 ctcgttcatc gaagccgatc gagaaaccgt cgcctgtccg cccgacgcgc cagccgtgaa
     1801 ttgctttggc gtgggccggg acaa
//
LOCUS       DATASH010000637         5026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2082101,
            whole genome shotgun sequence.
ACCESSION   DATASH010000637 DATASH010000000
VERSION     DATASH010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5026
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2082101"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="VHV99_17305"
     CDS             complement(<1..496)
                     /locus_tag="VHV99_17305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEX3637206.1"
                     /translation="MISYAVWLYYRFPLSPRMVEELLSVRGIDLTHETVRRWSVKFDS
                     GNARRLGATVLAGGDKWHLDEVVVTINGKKHWLWRAVDQYGAVLDVMVLSRRDRHAAR
                     RLMRNRLEKHGRAPRVLITDRLKSYAAANRDLGLNVEHRQHKGLNNRAENSHQRTRVR
                     EKVMR"
     gene            783..2459
                     /gene="treA"
                     /locus_tag="VHV99_17310"
     CDS             783..2459
                     /gene="treA"
                     /locus_tag="VHV99_17310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreA"
                     /protein_id="HEX3637207.1"
                     /translation="MIVTSKVHCSVATALPSSRPAAGTSRQRSAKQAAAVSGFVILTC
                     LSNVCFADTQLGGTLPPPPDKLYGDLFVAVQIGQIYPDQKTFVDATPNADPAAIVQLY
                     EQQKNNPGFSLASFVNQYFTPPSEPVITPPPNQTLREHINWLWPALTRTTASAPANSS
                     LIPLPKPYVVPGGRFREGYYWDTYFTMLGLQESGREDLVDDMLDNFAYEIDTFGHIPN
                     GNRTYYLDRSQPPFFSYMVTLAAKVEGGKVYQKYLPALRKEYAYWMQGAGSTRPGDAT
                     RNVVVMPDHTVLNRYWDELDTPRDESYLEDVQTAQQATGRTPGEVYRELRATAESGWD
                     FSSRWFGDNMTLATVRTTSIIPVDLNSLLFHLETTIAQGCGETRDFRCVGEFAERAVK
                     RAVGINRYLWNANGYYGDYDWKLGKPRDNKTAAMLYPLFVGAAWPERAQKTAHQVQST
                     LLQPGGLVTTTYNTTQQWDAPNGWAPLHWIAIEGLTRYGQDALAQQVGTRFLTDVKGV
                     YASDKKLVEKYVVEGAGTGGGGGGEYPLQDGFGWTNGVTLKLLDLYSPGE"
     gene            2862..3008
                     /locus_tag="VHV99_17315"
     CDS             2862..3008
                     /locus_tag="VHV99_17315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637208.1"
                     /translation="MGLVMVMPVNPKITGSFIIPARLRITGWTATIAMGAATLAMFWF
                     RLAS"
     gene            3459..3755
                     /locus_tag="VHV99_17320"
     CDS             3459..3755
                     /locus_tag="VHV99_17320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEX3637209.1"
                     /translation="MESTKGEGTLREIAGDVQQAAGDLLGNAGAQVSGTAKELSGKAQ
                     QLYADFAEVVRESTVERPFAALAIAAGVGFILGTLRATNRSGADNDRGDRRDRE"
     gene            3862..4236
                     /locus_tag="VHV99_17325"
     CDS             3862..4236
                     /locus_tag="VHV99_17325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018418324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX3637210.1"
                     /translation="MSMRSKVTKWRNVGRFCIERATDYGELLGVELEETRKCLVRELS
                     ALVALAVAGLFTLSFVCIAIIATAWGTPYFLPVVWGVAGMWLVVSVVCVLIVRSQKPA
                     RSFNVLQGELRNDLDTLKEALK"
     gene            complement(4782..>5026)
                     /locus_tag="VHV99_17330"
     CDS             complement(4782..>5026)
                     /locus_tag="VHV99_17330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEX3637211.1"
                     /translation="GDTPSMQRCGVCDNCMNPPYIALAEQSIAVDDTLRDADASKPSS
                     RPHPWSPGDAVRVPRFGAGEVALANGEQVVIVFPDG"
BASE COUNT          997 a         1535 c         1534 g          960 t
ORIGIN      
        1 ggcgcatcac tttctcgcgc acccgcgttc gctggtgcga gttctccgcc cggttgttca
       61 gtcctttgtg ctgccggtgt tccacgttca gcccaaggtc cctgtttgcc gcggcgtaac
      121 tcttgagcct gtcggtgatc agcacacggg gagcgcgacc atgcttctcg agccggttgc
      181 gcatgagccg cctggcggcg tgcctgtcgc ggcggctcag caccattacg tcgagcaccg
      241 caccgtactg gtctaccgca cgccacagcc agtgcttttt gccgttgatc gtcacgacca
      301 cctcatcgag gtgccactta tcgcctccgg ccagcacggt agcgccgagg cgccgggcgt
      361 taccagaatc gaacttcacc gaccagcgac gcaccgtttc atgggtaagg tcaattccgc
      421 gaactgacag caactcctcg accatacgcg ggctcaacgg aaaacggtag tacagccaca
      481 ccgcataact gatcatgtcg ggcggaaatc ggtagccggc ataagagata gcgtcgtcgg
      541 caggtgcgct gtgttggaga gcagttcgct tcaaacccac aattgtcgct gcagtttcgg
      601 caatatccca gcaacgtgac agtgccatca cgagcgcgac gccaggccgc ctgcttcatc
      661 gcgtccacgc gaggatttga accgtgcccg attctatgcc atgctgctct cgcccaacat
      721 cgttagaaaa atgagggcgt gcgccggaga ccagtcctgt tgtgcattcc ttcggaggag
      781 acatgattgt tacgtcgaaa gttcactgca gcgttgccac cgccctgcct tcatcacgcc
      841 ctgcagccgg cacgtctcga caacgaagcg cgaagcaggc ggcggccgtt tcgggattcg
      901 ttatcctcac ctgtctatct aacgtctgct tcgcagacac ccagctcggt ggcacgcttc
      961 caccgcctcc ggacaagctg tacggcgatc tgttcgtagc ggtccaaatc ggacagatct
     1021 acccggatca gaagacattc gtcgatgcaa cgccgaacgc ggaccctgcc gcgatcgtcc
     1081 agctgtatga acagcaaaag aacaaccccg gtttttcgct ggcaagcttt gtcaaccagt
     1141 acttcacacc gcccagcgag ccggtcatca ctccgccccc gaatcagacg ctgcgcgagc
     1201 atatcaactg gctgtggccc gcgctcaccc ggaccacggc gagcgcgccg gcgaacagtt
     1261 cgctgattcc attgccgaaa ccctacgtcg tgccaggcgg acgcttccgc gagggctact
     1321 actgggacac ctatttcacg atgctggggc tccaggaatc cggccgagag gatctcgtcg
     1381 acgatatgct cgataacttt gcgtacgaga tcgatacatt cggccacatt ccgaacggca
     1441 atcgtacgta ttacctggac cgttcgcagc cgccgttttt ctcgtacatg gtgacgctcg
     1501 cggcgaaggt cgaaggcggc aaggtgtatc agaaatatct gccggccctg cgcaaggagt
     1561 acgcgtactg gatgcaggga gcgggctcga cccgcccggg cgacgcgact cgcaacgtcg
     1621 tggttatgcc ggaccatacg gtgctcaacc gctattggga tgagctcgat acaccgcgtg
     1681 acgagtcgta cctggaggac gtgcagactg cgcagcaggc cacaggccga actccaggcg
     1741 aggtttatcg tgagctgcgg gcgacagccg aaagcggttg ggatttcagc tcacgctggt
     1801 tcggcgacaa catgacgctc gcgacggtcc gcaccacgtc gatcatcccc gtcgatctga
     1861 acagcctcct gttccacctg gaaacgacca ttgcgcaagg ctgtggcgag acccgcgatt
     1921 tccgttgtgt gggcgagttc gccgagcgcg cggtcaaacg cgcagtgggt atcaaccggt
     1981 acctgtggaa tgctaacggc tattacgggg actacgactg gaagctcggg aagccgcggg
     2041 acaacaagac ggcggcgatg ctgtatccgc tgttcgtagg tgcagcatgg ccggaacgcg
     2101 cgcagaagac ggcccaccag gtgcaatcga cgcttctgca gccgggaggg ctcgtgacga
     2161 ccacgtacaa cacgacacag caatgggacg cgccgaatgg ctgggcgccg ctgcactgga
     2221 tcgcgatcga aggtctgacg cgttacgggc aggatgcgct cgcgcagcag gtcggcacgc
     2281 ggtttctgac ggacgtaaag ggcgtctatg cgtccgataa gaagctggtc gagaagtacg
     2341 tggtagaagg cgcgggaacc ggcggcggag gcggaggcga atatccgttg caggacggct
     2401 tcgggtggac caacggcgtg acgctcaagc tgctcgatct gtatagcccc ggcgagtagt
     2461 gtccggtacg ggaaggggcg cgcacagtta gcagctgatg tgtgccccgc cgttcttgaa
     2521 acagaagggt acggtttgca caacgcgggg caaaggcttg ctgaaaggca tacaagacgc
     2581 ttgattgccc tgatccggcg caggcaacgt cgcatgccga ccccgtaatg atcgaacatg
     2641 cgtctccgcc gtatgtcttc gagacggcct gaacgagaca ttcgcgacac ctccgaatgc
     2701 taaacacatg aggccccgat gatgtcgtcg gacgcagtga attgcagggc acgggcgaag
     2761 tgctgcgggt aaaaggcgag cagggatcgt cggagacaac gctcagcgtc ggccgctgac
     2821 tggagcgcgg tgatcgatgg ggtggttttt ccgtcccgaa catgggactg gtcatggtca
     2881 tgccggtgaa tccgaagatc acgggcagct tcatcattcc agcacgcctg cgcattacag
     2941 gctggactgc aacgattgcg atgggcgctg ccacgctcgc catgttctgg ttcaggctcg
     3001 cctcgtgagg gcgtgagcga tgcgggcgcc attgcgttcg ttcccgtctc ccggaccgcg
     3061 ctgcaacagc cacttcgccg aggtgcagag gcactgttga aagccgcgac aatgtggtcg
     3121 gacccattca ggccgcctct caggacaagt gctgattgcg cgacgcagac ccgcttatgc
     3181 cctctccatg gcattgcatt tgagctaatc ccctcgacga cgcactcggc cttccgcgtc
     3241 agatccgacg aaatcttgcc ggaaactgga aggcggcata agtcctgcac gtcctatgcg
     3301 cggctcaatg gattatcggg ttgggcccag gtcttgcgca acgattggca cagcatcacg
     3361 ctgccgggtc cgcgctttgc acagaagcgt ggttcttggc tttcacggcg ccgcattttt
     3421 cccgtgcggc ctgatcgcac ccagttgagg agcagacaat ggaatcgacg aaaggagaag
     3481 gaacgctacg agagatcgcc ggcgatgtgc agcaggccgc aggggatctc ctcggcaatg
     3541 ccggtgcgca agttagcgga acggcaaagg aactgagtgg caaagcgcag caactctatg
     3601 ccgattttgc ggaggtggta cgcgagtcca ccgtggagcg gccgttcgcg gcgctggcga
     3661 ttgccgctgg cgtgggtttc atcctcggca cgttacgcgc gacaaatcgc tccggggccg
     3721 ataacgaccg cggcgatcgg agagatcgcg aataaggcga ttcgcgtctt gccctccaca
     3781 acaccgaaga ggtgcagtcg gcaagcacgc ggacgatcgc gacggcgttg tcgcagcacg
     3841 ccagccctga ggagtcccgc aatgtcaatg cgttcaaaag tcacgaagtg gcgaaacgtc
     3901 ggcagattct gcatcgagcg agccaccgat tatggtgagc tcctcggtgt cgaactcgag
     3961 gaaaccagga aatgtctggt ccgtgagtta agtgcactgg ttgccctggc cgtcgcgggc
     4021 cttttcacgc tctcgtttgt gtgcatcgcg attatcgcaa cggcttgggg gacgccttat
     4081 tttcttcccg tggtttgggg tgtcgccggg atgtggcttg ttgtgtctgt tgtctgcgtg
     4141 ctaatcgttc gctcccagaa accagcgcga tcgtttaatg ttctgcaggg ggaactccgg
     4201 aacgatctcg acaccttaaa ggaagcgctt aaatgaacca tgcacctggc gacgatgaaa
     4261 ccgagaccgc catcctgctg cgcatggcgg catcacgcgc agcgctgctc gcagcgaaca
     4321 gcactccacc atcggtcccg gctgtacatg gacagatgcg gtctcgcgcg gccggcctga
     4381 tcgcttcgct ggcagacgca ccgcgcgtta cgctgctgct agcgctttgc gttagcgcga
     4441 tcgtcctcgg acctgggcgg acagttgcaa tcgccagccg ttcggggatt gctgcgtggc
     4501 taggcgacac cgtccggaaa gtaatccaca aggctgtatg agcagttgcg gcgtagccat
     4561 agcgcggccg ccctgtccgc cactactact cttccacccg ttccaccacc accgcctccc
     4621 gccccttgcg cgaaggcgga gcaggcgaac gcgaacagaa ctgccgaata aactgccaac
     4681 aacgtttggc gctggacctt tttcatcacg tcaatcgtca cacaggtgag ctagaccgcg
     4741 tggcgggctg cctttacata gctcgacatg aaggtccgcg ttcaaccgtc gggaaacacg
     4801 atcacgacct gctcgccgtt cgccaaagcc acctctcccg ccccaaacct cgggacgcgt
     4861 accgcgtcgc ccggtgacca tggatgcggc ctcgacgatg gtttcgatgc gtccgcgtcg
     4921 cgcagcgtgt cgtccacggc gatcgactgc tccgccagcg caatgtacgg tggattcatg
     4981 cagttgtcgc agacgccgca gcgctgcatc gacggcgtat caccgt
//
LOCUS       DATASH010000638         5218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2281661,
            whole genome shotgun sequence.
ACCESSION   DATASH010000638 DATASH010000000
VERSION     DATASH010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5218
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2281661"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..111
                     /locus_tag="VHV99_17335"
     CDS             <1..111
                     /locus_tag="VHV99_17335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017334572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliG C-terminal domain-containing protein"
                     /protein_id="HEX3637212.1"
                     /translation="RVSEVETQQRRILQIVRNLAESGQIVLGGKAEDAYV"
     gene            98..781
                     /gene="fliH"
                     /locus_tag="VHV99_17340"
     CDS             98..781
                     /gene="fliH"
                     /locus_tag="VHV99_17340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HEX3637213.1"
                     /translation="MHMSDQNASAKERLSAYQRWEMASFDPVPPAPPEPEVDDGAFEA
                     ELQRLRDAAHAQGVATGHVAGQALGYQAGYDQGHAQGFAQGQSEAREEAARLAALAEP
                     FRRALEGAQGAISETLVALALDIAQQVVRQHVQHDPTALIAAAREVLATEPALIGAPA
                     LIVSPADLPVVEAYLMEELQTRGWTVRTDPSVERGGCRAQAGTGEVDAGIDTRWERVA
                     GALGRVSTW"
     gene            775..2436
                     /gene="fliI"
                     /locus_tag="VHV99_17345"
     CDS             775..2436
                     /gene="fliI"
                     /locus_tag="VHV99_17345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HEX3637214.1"
                     /translation="MVKPTLEEIRASDLTPLERELALASFGAEALVDIPAAATPAAAA
                     IAAIDAALRDPGAGHPATGAAASRESAAASRESASSSPASSAAAHAPYDPALDSNPHV
                     QAWRGRLDALRARSAIAKPMRACGRLTRAAGLVLEAVGLRLSVGADVMIELPPGSSLP
                     MAEAEVVGFSGDKLFLMPTTEVIGLLPGARVYPLESAPIADPMAGAKRLPVGWELLGR
                     VLDASGRPLDGLGPLGTHADAPLSAPVINPLNREPIHQVLDVGVRAINALLTVGRGQR
                     MGLFAGSGVGKSVLLGTMARYTSAEVIVIGLIGERGREVKEFIEQILGEEGLARSVVI
                     AAPADVSPLLRMQAASYSTSLAEYFRDQGKHVLLLMDSLTRYAMAQREIALAVGEPPA
                     TKGYPPSVFAKLPALVERTGNGPAGGGSITAFYTVLTEGDDQQDPIADSARAILDGHI
                     VLSRSLAEAGHYPAIDIEASISRAMTALIGDNHLEKTRMFKQMLSRYQRNRDLINVGA
                     YSSGRDALLDRAIALYPRMEAFLQQGFRECANFKPSLEMLDALFA"
     gene            2448..2897
                     /gene="fliJ"
                     /locus_tag="VHV99_17350"
     CDS             2448..2897
                     /gene="fliJ"
                     /locus_tag="VHV99_17350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006053241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HEX3637215.1"
                     /translation="MAKHFPIKTLIGLAQDDVDAAAQRLGRAQRERNDVQSQLDALVQ
                     YRDEYHTRFTATAQAGMPAGNMRNFQAFIDTLDTAIEQQRNLLVTANARVEAAKPDWQ
                     RQKQKLGSYEVLQARGEAAEAKTVARRDQRDADEHAARILRMRTEGA"
     gene            2959..4596
                     /locus_tag="VHV99_17355"
     CDS             2959..4596
                     /locus_tag="VHV99_17355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012402286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HEX3637216.1"
                     /translation="MSPLSPIGSLPGSASSTSNSAAIGAAANALSFSQTLQQNIQLQN
                     NLAMQSASPPPASSSSVHDAPPPPDPSTKSPDTGSSNTDKNNSTNASSSASSGSSGSS
                     GSSASSASSASSGSSASSGSSSASSGSSSALSASSASSGSSPASSASSASSANTQQAS
                     TDKTKATLPKSGNPTTQTDAGTAAAAAAAQAQAQANANNATDPASADPGTALTDAATA
                     LPGTGTDTDTTTGGTTGKKPGATDPKSAQDALQAALAALANGQGVVPAQALASGAAAH
                     AAGGLAASANNGLNGSADGKTLATGLFGEGKGSSAGKAALTTAATAMADLSAAAKAAP
                     DAQSTPAGGDARGGDLASFNSATDAATAALAASQAAAASAASTTAAMQTTGNAGAAEA
                     ANALSPPVGTTDWEDALSQKVVFLSNANSQSAELTLNPKDLGPLQVVLQVADNHAHAL
                     FVSQHQQVREAVEAALPKLREAMESNGIGLGSASVSDGFARQGSGQQSADSGQSGRGG
                     SGAAGTFASSAGGDSESVDNGGSGAVRRSVGLVDTFA"
BASE COUNT          856 a         1670 c         1812 g          880 t
ORIGIN      
        1 cgcgtctccg aagtcgagac gcagcagcgc cgcatcctgc agatcgtgcg caatctggcg
       61 gaaagcggcc agatcgttct aggcggcaag gcggaagatg catatgtctg atcaaaatgc
      121 ctcggcgaag gaaaggctct cggcctacca gcgctgggag atggcctcct tcgatccggt
      181 gccgcccgcg ccgcccgaac ccgaagtcga cgacggcgca ttcgaagccg aactccaacg
      241 tctgcgcgac gccgcgcatg cgcagggcgt cgcgacgggt cacgtggccg ggcaggcgct
      301 cggttatcag gccggttacg accagggcca cgctcaaggt ttcgcgcagg gccagagcga
      361 agcacgcgaa gaagcggcgc gcctcgccgc gctggccgag cccttcagga gggcactcga
      421 gggcgcgcag ggcgcgatct cggagaccct ggttgcgttg gcgctggata tcgcgcaaca
      481 ggtggtacgc cagcatgtgc agcacgatcc gaccgcactg attgccgccg ctcgcgaggt
      541 actggctacg gagccggcgc tgatcggggc gccggcgctg atcgtcagcc ccgccgacct
      601 gccggtcgtc gaagcttatc tgatggaaga actgcaaacg cgtggctgga ctgtgcgcac
      661 cgatccctcg gtcgaacgcg gcggctgccg cgcgcaagcc ggcaccggtg aggtcgatgc
      721 cggtatcgac acgcgctggg agcgcgtcgc cggcgccctc ggcagggtga gcacatggtg
      781 aagccgacgc tcgaagaaat ccgcgccagc gatctgacgc cgctcgaacg cgagctcgcg
      841 ctggcgtcgt tcggcgccga ggcgctggtg gatataccgg cagcggctac gcccgctgcg
      901 gctgcaatcg cggccatcga cgctgcgttg cgtgatccgg gggccggcca tccggcaacg
      961 ggcgcagcgg caagccgcga atccgctgcc gcgagccgtg aatcggccag ttcgagcccc
     1021 gcttcaagcg ccgccgccca cgccccgtac gatcccgcac tcgacagcaa cccgcacgtc
     1081 caggcctggc gcgggcggct cgacgcgctg cgcgcgcgca gcgcaatcgc caagccgatg
     1141 cgtgcctgcg gacggttgac gcgcgccgcc ggcctggtgc tcgaagcggt cggcctgcgc
     1201 ctgtcggtgg gcgccgacgt gatgatcgaa ctgccgcccg gcagttcgct gccgatggcc
     1261 gaagccgaag tggtcggctt ctccggcgac aaactgtttc tgatgccgac taccgaagtg
     1321 atcggtctgt tgcccggcgc gcgtgtctac ccgctcgaaa gcgcgccgat cgccgatccg
     1381 atggcgggcg cgaagcgctt gccggtcggc tgggaattgc ttggccgcgt gctggacgcg
     1441 tccggccgcc cgctcgacgg cctcggcccg ctcggcacgc atgccgacgc gccgctgtcc
     1501 gcaccggtca tcaacccgct gaaccgcgag ccgattcatc aggtgctcga cgtcggcgtg
     1561 cgcgcgatca acgcgcttct gactgtcggc cggggccagc gcatggggct gttcgccggt
     1621 tcgggcgtgg gtaaatcggt gctgctcggc accatggcgc gctacaccag cgccgaggtg
     1681 atcgtgatcg gcctgatcgg cgaacgcggc cgcgaagtga aggaattcat cgaacagatt
     1741 ctcggcgagg aaggcctcgc gcgctcggtg gtgattgccg cgcctgccga cgtctcgccg
     1801 ctcttgcgga tgcaggccgc gtcgtactcg acctcgctgg ccgaatattt ccgcgaccag
     1861 ggcaagcacg tcctgctgct gatggattcg ctgacccgtt acgcgatggc gcagcgcgag
     1921 atcgcgctgg ccgtgggcga gccgcccgcc accaagggct atccgccttc ggtgttcgcc
     1981 aagctgccgg cgctcgtcga gcggaccggc aatggtccgg cgggcggcgg ctcgattacc
     2041 gcgttctata ccgtgctgac cgaaggcgac gaccagcagg acccgatcgc cgactccgcc
     2101 cgcgcgattc tcgacggcca tatcgtgctg tcgcgctcgc tcgccgaggc cggccactac
     2161 cccgcgatcg atatcgaagc gtcgattagc cgggcaatga ccgcgttgat cggcgataac
     2221 catctcgaga agacgcgtat gttcaagcag atgctgtcgc gctatcagcg caaccgcgat
     2281 ctgatcaacg tcggcgccta ttcgagcggg cgcgacgcgc tgctcgaccg ggcgattgcg
     2341 ctgtatccgc gaatggaagc atttttgcag caaggttttc gcgaatgcgc gaactttaaa
     2401 ccgagtctcg agatgctgga cgcgctgttt gcctaaggag gctgacgatg gcgaaacact
     2461 ttccgatcaa gacactcatc ggcctggccc aggacgacgt ggatgccgcc gcacaacgtc
     2521 tgggccgcgc gcagcgcgag cgcaacgacg tgcagtcgca gctcgacgcg ctggtacagt
     2581 accgcgacga atatcacacg cgcttcacgg cgactgccca ggcgggcatg cccgccggca
     2641 acatgcgcaa tttccaggca ttcatcgaca cgctcgacac cgccatcgaa cagcagcgca
     2701 acctgctggt gacggccaat gcgcgcgtcg aagctgccaa gcccgactgg cagcgccaga
     2761 agcagaagct cggttcgtat gaagtgctgc aggcgcgcgg cgaggctgcc gaggcgaaaa
     2821 ccgtggcgcg gcgcgaccag cgcgatgccg acgaacacgc cgcccgcatt ctgaggatgc
     2881 gcaccgaagg cgcttgacgc gccccggcgc cgcctcgatt gacggaacac cccgtttgac
     2941 ggattgacag gattccctat gtcgcctctt tcaccgatcg gctcactgcc tggctccgcc
     3001 agcagcacgt ccaacagcgc tgccatcggc gctgcggcga acgccctatc gttctcgcaa
     3061 actctgcaac agaacatcca gctgcaaaac aatctggcca tgcagagtgc gagcccgccg
     3121 ccggcttcgt cgtcgagcgt gcatgacgca ccaccgccgc ccgatccttc gacgaagagc
     3181 ccggataccg ggagcagtaa cacggacaag aacaattcga cgaacgcttc gtcgtctgct
     3241 tcgtctgggt cgtctgggtc gtctgggtcg tctgcttcgt ctgcttcgtc tgcttcgtct
     3301 gggtcgtctg cttcgtccgg gtcgtcgtcc gcttcgtccg ggtcgtcgtc tgccttgtct
     3361 gcttcgtctg cctcgtccgg gtcgtcgccc gcttcgtctg cttcgtccgc ttcgtctgcc
     3421 aatacgcagc aggcctcgac cgacaagacc aaagccacgc tgccgaagtc cggcaacccg
     3481 accacgcaga ccgacgcggg cacggccgca gcggcggccg ctgcgcaggc ccaggctcaa
     3541 gccaatgcga ataacgcgac cgacccggcg agcgccgacc ccggcacagc actgactgac
     3601 gccgcaacag cactgcccgg cacgggcacg gacacggaca ccacaacggg cggcaccacc
     3661 ggcaaaaagc ctggcgcaac cgaccctaag tcggcgcagg acgccttgca ggcggcgcta
     3721 gccgcgctgg cgaacggcca gggcgtggtg ccggcgcagg cgctggcgag cggcgcggcg
     3781 gcgcacgcgg caggcggtct ggcggctagc gcgaataacg gactgaacgg atcggcggac
     3841 ggcaagacgc ttgcgacagg attgttcggc gagggcaagg gatcgagcgc tggcaaggcc
     3901 gcgttgacga cggcggccac ggccatggcc gatctgtcgg cggcggcgaa ggctgcgcct
     3961 gacgcgcaga gcacgcccgc tggcggcgat gcgcggggtg gcgatctggc gtccttcaat
     4021 agcgcgaccg atgccgcgac ggcggcgctg gcagcgagcc aggccgccgc ggcgagcgct
     4081 gcgagtacga cggcggccat gcaaacgacg ggtaatgccg gcgcggcgga agcagcgaat
     4141 gcgttgtcgc cgccggtggg tacgacggac tgggaagatg cgttgagtca gaaggtggtg
     4201 tttttgtcga atgcgaattc gcagagcgcc gagctgactt tgaatccgaa ggatctggga
     4261 ccgctgcagg ttgtgttgca ggttgccgat aaccatgccc atgcgttgtt tgtatcgcag
     4321 catcagcagg tgcgggaggc ggtcgaggcc gcgttgccga agcttcgtga ggcgatggag
     4381 tccaatggga ttgggttggg gagcgcgagc gttagcgatg ggtttgcccg ccaggggagt
     4441 gggcagcaga gtgctgattc ggggcagtca gggaggggag gcagcggggc tgcggggact
     4501 tttgccagca gcgctggggg tgattctgag tccgttgata acggagggag tggtgccgtg
     4561 cggcggagtg tgggattggt ggatacgttt gcttagtcgt ttttttgcct gggcggcggt
     4621 ttttgtttgt gtttttttgg cgttggactt tgcttgatct ggttgcccgt gcggcgcttt
     4681 gtggctgtgc gcctacggtg ttggcctttc cttgatttct tagtggtcta gtagcgttgc
     4741 ccctgtgcgg ggcggcacct acttttcttt gcctgccgca aagaaaagta ggcaaaagaa
     4801 agcggctcac cccgccagct cataagcggg tcccccacgc agtcgcggta gtggtgcatc
     4861 tggaatccgt gccatcgcac attcggcgtg agtgaccagg ccgtcatcag cacccactcc
     4921 gcactacgtg cgtcgcggat gggtatgcat gggaaaccag gggcctcgtt tcggcgtggt
     4981 gggggccgtc ggcttcgcct cggcgcggac gggtttgcat gggaaaccag gggcttcgtt
     5041 tcggcgcggt gggggccgtc ggcttcgccg cggcgcggac gggtttgcat gggaaaccag
     5101 gggcttcgtt tcggcgcggt ggggccgtcg gcttcgcctc ggcgcggacg ggtttgcatg
     5161 ggaaaccagg ggcttcgttt cggcgcggtg aggcatcggc ttcgcctcgg acaggact
//
LOCUS       DATASH010000639         1681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5644673,
            whole genome shotgun sequence.
ACCESSION   DATASH010000639 DATASH010000000
VERSION     DATASH010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5644673"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1681
                     /gene="flhA"
                     /locus_tag="VHV99_17360"
     CDS             <1..>1681
                     /gene="flhA"
                     /locus_tag="VHV99_17360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018418777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HEX3637217.1"
                     /translation="LVSMYTMKPLDFAAFPSVLLFSTLLRLSLNVASTRIVLLEGHTG
                     PAAAGQVIESFGHFLVGGNFAVGIVVFVILMVINFMVITKGAGRIAEVSARFTLDAMP
                     GKQMAIDADLNAGLINEDQARKRRLEVSQEAEFYGSMDGASKFVRGDAIAGLLIMVIN
                     ILGGLVVGMVQHGMDFASAGKTYTLLTIGDGLVAQIPSLVISTAAGVIVSRVATNEDI
                     GTQLTGQLFTNPRVLVITGSILVLMGLIPGMPHFAFLILGGGLIQLGRTMKKRSEDRK
                     TGAALVDVTPAAIAPVENTEASWDDVTMIDTLGLEVGYRLIPLVDQNSDGELLKRIKS
                     IRKKFAQEIGFLPPVIHIRDNLELRPNGYRIALKGVEVGVGEAYPGQWLAINPGQVSA
                     ALPGTPTQDPAFGLPAIWIDTNLREQAQVYGYTVVDSSTVVATHLNHLVVAHASELLG
                     RREVQTLVERMQKDTPSLVDDLVPKSLPLTTLQKVLQNLLEEGVPIRDLRTILEALSE
                     HAPKITDAHELTAAVRLALGRAITQQWFPGVGDMQVMGLDPNLERVLSQALS"
BASE COUNT          297 a          526 c          557 g          301 t
ORIGIN      
        1 gctggtcagc atgtacacga tgaagccgct cgacttcgcc gccttcccga gcgtgctgct
       61 gttctcgacc ttgctgcgcc tgtcgctgaa cgtcgcgtcc acgcgcatcg tgctgctcga
      121 aggccatacc ggcccggccg ctgccggcca ggtgatcgag tcgttcggcc acttcctcgt
      181 gggcggcaac ttcgcggtcg gtatcgtcgt ctttgtcatt ttgatggtca tcaacttcat
      241 ggtgatcacc aagggcgcgg ggcggatcgc ggaagtgtcc gcgcgcttca cgctcgacgc
      301 gatgcccggc aagcagatgg cgatcgacgc cgacctgaac gccggcctca tcaacgagga
      361 tcaggcccgc aagcgccggc tggaagtctc ccaggaagcc gagttctacg gttcgatgga
      421 cggcgcgagc aagttcgtgc ggggcgacgc gatcgccggt ttgctgatca tggtgatcaa
      481 catcctcggc ggcctggtcg tcgggatggt tcagcacggc atggacttcg catccgcggg
      541 aaagacctac acgctgctga cgatcggcga cgggctcgtc gcgcagatcc cgtcgctcgt
      601 gatttcgacg gcggccggtg tgatcgtttc gcgcgtcgcg accaacgaag acatcggcac
      661 gcaattgacg gggcagttgt tcacgaatcc gcgcgtgctg gtgatcaccg gcagcattct
      721 cgtgctgatg ggcctgattc caggcatgcc gcacttcgcg tttctgattc tcggcggcgg
      781 gctgatccag ctcggccgca caatgaaaaa gcgcagcgag gaccgcaaga ccggcgcggc
      841 gctggtcgac gtcacgccgg ccgcgatcgc gccggtcgaa aacaccgaag ccagctggga
      901 cgacgtgacg atgatcgaca cgctcggtct cgaagtcggc taccgcctga tcccgctggt
      961 cgaccagaac tcggacggcg aattgctgaa gcggatcaag agcatccgca agaagttcgc
     1021 gcaggaaatc ggcttcctgc cgccggtgat ccatatccgc gacaacctcg aattacggcc
     1081 gaacggctac cggattgcac tcaagggcgt cgaggtgggc gtcggcgaag cgtatcccgg
     1141 gcagtggctg gcgatcaatc cggggcaggt ctccgccgcg ctgccgggta cgccgacgca
     1201 agacccggcg ttcggtttgc cggcgatctg gatcgatacg aatctgcgcg aacaggcgca
     1261 ggtgtatggc tatacggtgg tcgattcgag caccgtggtg gctacgcacc tgaatcacct
     1321 ggtggtcgcg catgcgtccg aactgctcgg ccgccgcgaa gtgcagacgc tggtggagcg
     1381 catgcagaag gacacgccgt cgctggtcga cgatctggtg ccgaagtcgc tgccgctcac
     1441 cacactgcaa aaggtgctgc aaaacctgct ggaagaaggc gtgccgatcc gcgatctgcg
     1501 cacgatcctc gaagcgctgt ccgaacacgc gccgaagatt accgacgcgc acgagctcac
     1561 cgccgccgtg cgcctcgcgc tgggccgggc gattacgcag cagtggttcc cgggcgtggg
     1621 cgacatgcag gtgatgggcc tcgacccgaa tctggagcgg gtgttgtcgc aggcgctttc
     1681 t
//
LOCUS       DATASH010000640         2051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4457757,
            whole genome shotgun sequence.
ACCESSION   DATASH010000640 DATASH010000000
VERSION     DATASH010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4457757"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..846
                     /locus_tag="VHV99_17365"
     CDS             67..846
                     /locus_tag="VHV99_17365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEX3637218.1"
                     /translation="MTRDPRLTLNARQQELLEWVQRDGFVTVDDLAAHFDVTPQTIRR
                     DVNWLADMNLLRRYHGGASLPTSSENVSYTARQRMFHDEKRRIATLVATHIPDQASLF
                     INLGTTTEEVARALNRHRGLRVITNNLNVASMMSGYPDCEVLVTGGIVRPWDKGIVGE
                     LAIDFIRQFKVDFAIIGTSSIETDGTLRDFDTREVRVAEAIIQHARTVFLAADNSKFG
                     RPALVRQGHLEQIDALFTDAAPPAEMNETLAAANCQVYVAE"
     gene            1165..1362
                     /locus_tag="VHV99_17370"
     CDS             1165..1362
                     /locus_tag="VHV99_17370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637219.1"
                     /translation="MLSPHEFATLLLVKDAPNQVDMERQELDALLERQLVQLERLASG
                     NEQWRVTETGDNALRAIKRLS"
     gene            1558..>2051
                     /locus_tag="VHV99_17375"
     CDS             1558..>2051
                     /locus_tag="VHV99_17375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX3637220.1"
                     /translation="MPKTLSSDDIQRFREAMRRVAENAFATRGAQGVTMRELAKELGC
                     SAMTPYRYFRDKDEILAMVRAAAFNRFAARLEAVAKQAPATDHSAVSDAYVAFALDEP
                     HAYRLMFDLTQPEAAYPELTAASQRAWQLLGVHFEQLVAAGILEGDPQLIGYAYWASL
                     HGFT"
BASE COUNT          415 a          681 c          596 g          359 t
ORIGIN      
        1 cttaccggcc cagcttggcg ggcctgcggc gggcaacgca gacaagaaac ccacgaaccg
       61 agacacatga cccgagaccc ccgcctgact ctcaatgccc ggcaacagga actgctggag
      121 tgggtgcaac gcgacggctt cgtgaccgtg gacgacctcg cggcccactt cgacgtgacg
      181 ccgcagacga tccgccgcga cgtcaactgg ctcgccgaca tgaacctgct gcgccgctat
      241 cacggcggcg ccagcctacc gaccagctcc gagaacgtct cgtacacggc gcgtcagcgc
      301 atgttccatg acgagaagcg gcgcattgcg acgctagtgg ccactcacat tcccgaccag
      361 gcgtccctct tcatcaacct cggtacgacc acagaggaag tcgcacgggc gctcaaccgg
      421 catcgcggcc tgcgcgtgat caccaacaat ctgaacgtcg ccagcatgat gagcggctac
      481 ccggattgcg aagtgctggt gacgggcggc atcgtgcggc cgtgggacaa gggcatcgtc
      541 ggcgaactgg cgatcgactt catccggcag ttcaaggtgg acttcgcgat catcggtacc
      601 tcgagtatcg aaacggacgg cacgctgcgc gatttcgata cccgcgaagt gcgcgtggcc
      661 gaggcgatca tccagcatgc gcgcacggta tttctcgccg ccgacaactc caaattcggc
      721 cgcccggcac tcgtccgcca aggtcatctc gagcagatcg acgcactttt caccgacgcc
      781 gcgccgcctg ccgagatgaa cgaaacgctc gccgccgcca actgccaggt gtatgtcgcc
      841 gagtaatccc acgcgcatcc aggcgacttc ggccatgttg cagcgcacgc gaaacttcga
      901 gtgaaatcaa ggatcttgcc gcggctccaa accgcctctg caggtcccaa ttgtcaaagg
      961 gaaaatttac tggggcccat ttggtgttta acggccgttt gactacactc cagttccccg
     1021 gcgtcgttcc gcgttacgcg gagtgccggt agcctgaacc tgtcgtgata gccgtaactc
     1081 ccgactgatc ctttagcgga cttcactggc atctggccag tcgccggcaa ggccgtccgc
     1141 gtttgcttga catggagaga aacgatgctg agtccgcatg aattcgccac gttgttgctc
     1201 gtcaaggacg cccccaatca agtcgacatg gagcgacaag aactcgacgc cctattggaa
     1261 cgccagctcg tgcaactcga acggcttgcg tcgggcaacg aacaatggcg cgtgaccgaa
     1321 accggcgaca acgcactccg ggccatcaag cgtctctcct agtacgccgg gtttgaaacc
     1381 ggcgacggca gcaggacggc gcgctgcgtt gcgataacat tgcacgcgtt cacgtcttcc
     1441 gggttgtcgc atatcttttt tgctcaatca agcaaggcgg ccgcgcaaaa cggccgcttt
     1501 attgtgccgg gttgatttac acagtaaatt gacgcgacgg accttttgcc ctcgcgcatg
     1561 cccaagacac tctcctccga cgacatccag cgattccgcg aagccatgcg gcgcgtcgcc
     1621 gaaaacgcgt tcgccacgcg cggcgcgcaa ggtgtgacga tgcgcgaact ggcaaaggag
     1681 ctgggttgca gcgcgatgac gccctatcgc tacttccgcg acaaagacga aatccttgcg
     1741 atggtccgcg ctgcagcgtt caaccgcttt gcggcgcggc tcgaagcagt ggcgaaacaa
     1801 gcgcccgcga ccgaccactc cgccgtcagc gacgcctacg tggccttcgc gctcgacgag
     1861 ccgcacgcct accgcctgat gttcgatcta acacagccgg aggccgccta cccggaactc
     1921 acggccgcgt cgcagcgcgc gtggcaactg ctcggggtgc acttcgagca gctcgttgcc
     1981 gcgggcattc tggaaggcga tccgcaattg atcggctatg cgtactgggc aagcctgcac
     2041 ggcttcacca t
//
LOCUS       DATASH010000641         1954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6829240,
            whole genome shotgun sequence.
ACCESSION   DATASH010000641 DATASH010000000
VERSION     DATASH010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6829240"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..1934
                     /locus_tag="VHV99_17380"
                     /pseudo
     CDS             1..1934
                     /locus_tag="VHV99_17380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014252704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
BASE COUNT          383 a          574 c          624 g          373 t
ORIGIN      
        1 cgacttcagc caatatgctg ccttcgatca gcgtcaaagg cgccgctgaa agcgaggcaa
       61 ccgtcggcct cgtcgcgcgc cgcagcagca ccgccaccaa gaccgacacg gcgctgaggg
      121 aaattccaca gacgatcaac gtcgtgaccg cacagcagat cgagcaaacc ggcgcgacga
      181 ccctcaacga agccttgcgc tacgttccgg gcttttcgac gtatggctcg gatgtgcgct
      241 cggactggta ttccgtactg cgcggcttta cgccgactgt tttcgtcgac ggtctgcagg
      301 tgcccaatac gctcaatctc tcgagctggc gtgtcgatcc gtacatgatc gacagcatca
      361 cggtgctgcg cggcccgact tccgttctgt acggacaggg cgatcctggc gcgatcatcg
      421 acgtgcagag caagctcgcg aacggcgagc gcacgcgcga aattgaaatg caattgggcg
      481 actacgcgcg caagcagctt gcattcgatg tgggcgacaa gatcgataag gacggcacgc
      541 tgtcgtatcg ctttgtcggc gtgggacgtg acggtaattc gcaaactggc ccgaatcccg
      601 agcaacgtgt ggcgctcgcg ccttcggtga agtggcagcc gaacgcgaag acttcgctga
      661 ccgtggaggc gagttatctg caagattggg gcgacgcatc gaataacttt ctgccggcgc
      721 agggcacgat tttgccgaat ccgaacggca agatttcacc ggatctctat acgggcgacc
      781 cggagttttc gcattaccgc aagaaacagt ggtcggtggg ttaccagttc gaatacaagt
      841 tcgatcccgt ctggaccttc cggcagaaca cgcgcctgat gcacctgtcg ctcgatcagg
      901 ggcaggtgtt cggcggcggt ctggatccgg ccgatcccac cgaggcgtcg ctgctgcgtt
      961 acgtcggcct gtatcagctg aactacagcc gcttcgacgt cgacaaccag gcgctggcca
     1021 gattccgcac cggtccgctc gaccatacgg tgctgctcgg ctttgaatac aaccggcagt
     1081 ccacgaccga tagcgagtcg atggcgaccg ggccgagtct gaatatgtac aacccggtgt
     1141 atgcgccgat caccaacgcg atcttcggcg gcccggatgc gttccctcgc accgatacga
     1201 agtcgacgct cgattcgttc ggcgtgtatg cacaggacca gatcaagtgg cagcgctggg
     1261 tgctgacgct cggcggccgc caggactgga gcgacaccac gcagaacgat ctcgcgggcg
     1321 gcatgcggct tacgcaggac gatcacgcgt tctcggggcg cgtcgggctc gtctatctcg
     1381 gtgactacgg gctttcgccg tatatcagct attcgacgtc gttcaatccg cttgtcggcg
     1441 tgaagatcgc gggcggcggc attgctgagc cgaccaaggg caaacagatc gaagcgggtt
     1501 tgcgttggca accggccggc aagaatctga tgctgaacgc ggcgatttat cagatcaacc
     1561 agaccaatgt cgtcacgcct gattttgcgc tcgatcctag tggtggcacg tcggtgcaga
     1621 ccggcgaagt gcgctcacgt ggcatcgagt tgagcggggt gggtaacgtc acgcgcaatt
     1681 tctcggtgat cgccgcgtat gtgtaccagg acgtgaaaaa catcaaggca ggcgacgaga
     1741 ccttgggcaa gtggccggtg gatattccgc ggccgcgtca gatggcgtcg ctgtggggtg
     1801 actggacgtg gcacgtgggg ccgctcgcgg gcctcggcgt tgctgcgggg atccgctatc
     1861 aaagcggcgc ggcgggtgct gccgacaact cgctgtatgt gccgagctac accgtgtacg
     1921 acgcggcgat tcactaagag atgcgtaatt ggcg
//
LOCUS       DATASH010000642         5075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4851338,
            whole genome shotgun sequence.
ACCESSION   DATASH010000642 DATASH010000000
VERSION     DATASH010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5075
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4851338"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1141
                     /gene="urtC"
                     /locus_tag="VHV99_17385"
     CDS             <1..1141
                     /gene="urtC"
                     /locus_tag="VHV99_17385"
                     /inference="COORDINATES: protein motif:HMM:TIGR03408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HEX3637221.1"
                     /translation="PRMNTFPDPRTRRFTLSAFATKWMGQSVEVGIVPVLVLLAIYLP
                     FAYFVIPESNALHLSLFGINLIGQIMCFALLALALDLVWGYAGILSLGHGIFFGIGGY
                     MMAIYLLNGAYADTHVLPDFLQYMGASDFPAVWKMLSRLDVTIFATIVVTVLVAGAFG
                     FVTFRSRINGVYLSIITQALTYALMLLLFINDVGLGGNNGMTGFTSLAGRKLAATSTQ
                     IGLAVCSCLLLVVAYVGVRFLARSSFGRALIAVRDDEARMRFLGYRTHTLKLFAWCLS
                     AVLAAFAGMLYVPQVGIVNPTIVSPQLSVEIAVWVAIGGRGTLIGAIVGAIVVNGLKF
                     WLTAQLPALWPFILAGVTLVIVVYFGNGLWGTLKAASSETRGGDQ"
     gene            1138..1893
                     /gene="urtD"
                     /locus_tag="VHV99_17390"
     CDS             1138..1893
                     /gene="urtD"
                     /locus_tag="VHV99_17390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018418409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HEX3637222.1"
                     /translation="MNQRQPATAVYISGLSVTFSGFAAITNLSMEFRYGEIRAIIGPN
                     GAGKTTLMDVITGKTRPASGEVFLNKVTNLATLDETSIARLGIGRKFQKPSVFEALRV
                     RENLELAIRNGRGYAEMVRSRLGAQQNRRIDEVLETIGLENDGPRIAGALSHGQKQWL
                     EIGMLLVADPQVILLDEPVAGMTDHETARTAELIARLKSPERAVVVIEHDMDFVGKIA
                     DLVSVLHEGQLLAEGSMERVRNDPRVIQVYLGR"
     gene            1896..2600
                     /gene="urtE"
                     /locus_tag="VHV99_17395"
     CDS             1896..2600
                     /gene="urtE"
                     /locus_tag="VHV99_17395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013696866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HEX3637223.1"
                     /translation="MLKVSALNQFYGSSHTLRDVSLNVADGACTVVLGRNGVGKTTLL
                     KCLVGLLPVKAGEIELAGQPLTRMPSHRRVLAGLGYVPQGREIFPQLSVEENLRIGAY
                     AGESNQTAKGKQFSFDDVFHTFPVLKTMRRRIAGNLSGGQQQQLAIGRALLTNPKLLI
                     LDEPTEGIQPSIVMEIEAVIRSLKGSLSILLVEQFFDFAQAVADDYVVLVRGQVVSAG
                     PGNQMEANNVRALISV"
     gene            2661..3656
                     /locus_tag="VHV99_17400"
     CDS             2661..3656
                     /locus_tag="VHV99_17400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018418407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEX3637224.1"
                     /translation="MNWLEQSIMHTRGVARGQVRETHELSESRQGTFHYTIGPYSTPV
                     MNIAPGDRVVVETRDAFDGKILTEQDLPSQKLRVPFLNPQSGPIMIEGAEKGDVVAVY
                     IESMLPRGDNPRGTCCLIPRFGALTGTEYTATLNEPLPEITRKIDVDEEGVYWSKRVT
                     LPYKPHIGTLSLSPEIDSINSLTPDSHGGNMDVPDMGPGSITYLPVRSEGARLFIGDA
                     HACQGDGEVCGTAVEYSSTTTIHVDLIKGWHIEWPRLETDELLMAIGSARPLEDATRI
                     AYRELVLWMEKEYGFDRWDAYMMLSQCGKVRLGNFVDPKYSVGAAISKKYLAPAA"
     gene            3669..4439
                     /locus_tag="VHV99_17405"
     CDS             3669..4439
                     /locus_tag="VHV99_17405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3637225.1"
                     /translation="MDLQLKGLKALITGGTKGIGRAIAQTLAAEGSDVALCARDGAAV
                     EQTVADIVQVTGGAVKVCGASVDVSNGPALQAWTREAAAQLGGIDIVVANVSALSIGS
                     DLESWRKEFETDLMGTVNLVDAALPFLEASKAASIIAISSVSGREIDFAAGPYGTFKA
                     ALIHYMQGLAYQLAPKGIRANAVSPGNVYFEGGVWDHIEHNDPKLFAEALALNPSGRM
                     ARPQEIANAVAFVASPAASFVSGANLVVDGALTRGVQL"
     gene            4575..>5075
                     /locus_tag="VHV99_17410"
     CDS             4575..>5075
                     /locus_tag="VHV99_17410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3637226.1"
                     /translation="MASHGAMAADPIRIGVPVGLSGANSVVAPSVVQAAQLAVDEINA
                     KGGILGRQVGLEVADDGSGAAGAQKAFDSLIFEKKVNALISMETSAARNGGLPIVTRG
                     KVPFIYTSFYEGRSCNRYMYVDAWVPDQQVAPIVDYFMKQQKAKTFFLIGSDYAFGRG
                     MLDFTKT"
BASE COUNT          973 a         1501 c         1621 g          980 t
ORIGIN      
        1 cccgcgtatg aatacgtttc ccgatccgcg taccaggaga ttcaccttgt cggcgttcgc
       61 aacgaaatgg atgggccagt ccgtggaggt cggcatcgtc ccggtgctgg ttctgctggc
      121 gatctatcta ccctttgcct attttgtgat tccggagtcg aatgcgctgc atctgtcgct
      181 gttcggcatc aacctgatcg gacagatcat gtgcttcgcg ctgctcgcgc tggcgctcga
      241 tctggtatgg ggttatgcgg gcatcctgag cctcgggcac ggcatcttct ttggcatagg
      301 cggctacatg atggccatct atctgctgaa cggcgcctat gccgacacgc atgtattgcc
      361 ggacttcctg caatacatgg gggcgagcga ctttcccgcg gtatggaaaa tgctgtcgcg
      421 gctggatgtg acgatcttcg ccacgatcgt cgtcaccgtg ctggtcgcgg gcgcgttcgg
      481 cttcgtcacg ttccgctcgc gcatcaatgg cgtgtatctg tcgatcatca ctcaggcgct
      541 cacgtatgcg ctgatgctgc tgttgttcat caacgacgtg ggcctcggcg gcaacaacgg
      601 catgaccggt ttcacgagcc tcgcgggccg taagcttgcc gctacgtcca cgcagatagg
      661 cctcgcggtg tgttcgtgcc tgctgctggt ggtggcctat gtcggcgtgc gctttctcgc
      721 gcgctccagt tttggcaggg ccctaatcgc ggtgcgcgac gacgaagcga ggatgcgctt
      781 tctcggctac cgcacgcata cgttgaagct cttcgcatgg tgtctctcgg cggtcctcgc
      841 ggcgtttgcc gggatgctct acgtgccgca ggtcggcatc gtcaacccga ctatcgtttc
      901 gccgcaactg tccgtcgaga ttgccgtgtg ggtggccatc ggtgggcgcg gcaccctgat
      961 cggtgcaatc gtcggcgcta tcgtcgtcaa cggactcaag ttctggctga ccgcgcaact
     1021 gccggcgctg tggccgttca ttcttgccgg cgtgacactc gtgatcgtcg tctatttcgg
     1081 caatggcctg tggggtacgc tcaaggccgc tagcagcgaa accagagggg gcgaccaatg
     1141 aatcagcgac aaccggccac cgccgtctac ataagcggct tgtctgtcac gttttccggt
     1201 ttcgcggcga tcacgaatct gtcgatggag tttcgatacg gtgagattcg cgccatcatc
     1261 ggtccgaacg gtgcgggcaa gacgaccttg atggacgtca tcacgggaaa gacccggccg
     1321 gcaagcggcg aggtctttct gaacaaggtc acgaacctgg cgaccctcga cgagacgtcc
     1381 atcgcgcgcc tgggcatcgg ccgcaagttc cagaagccaa gcgtgttcga agcgctgcgg
     1441 gttcgcgaaa acctcgaact ggcgattcgc aacggtcgtg gctacgcgga gatggttcgc
     1501 tcgcgcctcg gcgcacagca gaaccggcgc attgacgaag tgctcgagac gattggtctc
     1561 gagaatgacg ggccgcggat tgccggcgcg ctgtcgcacg gccagaagca gtggctcgaa
     1621 attggcatgc tgcttgtcgc cgatccgcaa gtgatcctgc tcgatgaacc ggttgccggt
     1681 atgaccgacc acgaaaccgc acgcaccgca gaactgatcg cgcggctcaa atcgcccgag
     1741 cgggcggtgg tggtgatcga acacgacatg gattttgtcg gcaagatcgc ggacctcgtc
     1801 agcgtgctgc atgaagggca gttgctggcc gaggggtcga tggagcgcgt gcgcaacgat
     1861 ccacgcgtga ttcaggttta tctcggacgg tgagcatgct gaaggtttcc gcactcaatc
     1921 agttttacgg cagcagccac acgttgcgcg acgtgtcgct gaatgtcgcc gatggcgcct
     1981 gcaccgtcgt gcttggccgt aacggcgtgg gcaagacgac cttgttgaag tgcctcgtcg
     2041 ggctactgcc ggtcaaagcc ggcgaaatcg aactggccgg ccagccgctg acccgcatgc
     2101 cgtcgcaccg gcgcgtactg gcgggtctgg gctacgtgcc gcagggacgc gagatttttc
     2161 cgcaactcag tgtcgaagaa aacctgcgga tcggcgccta cgcgggcgag agcaatcaga
     2221 ccgccaaggg caagcaattt tccttcgacg acgtcttcca tacctttccc gtgctgaaga
     2281 cgatgcgccg ccgcattgcg gggaatctgt ctggcggaca gcaacagcag cttgcgattg
     2341 gacgggcgct tctgaccaat ccaaagctgc tgattctcga cgagccgacc gagggcattc
     2401 agccgtcgat cgtgatggag atcgaggcgg tgatccgttc actgaaagga tcgctatcga
     2461 tcctgctggt agaacaattt ttcgattttg cgcaggcagt agccgacgac tatgtcgtgc
     2521 tggtacgcgg ccaggtcgtg tcggcggggc cgggaaacca gatggaagcc aacaacgtca
     2581 gagcactaat ttcagtgtga tgcggcgcca gcgttgcgcc cattcacttc gacaacccac
     2641 atttgaacag gaacaccacg atgaactggc tagaacaatc gatcatgcac acgcgcggcg
     2701 tcgcacgcgg tcaggtccgc gaaacgcatg aactcagcga atcccgtcag ggcacctttc
     2761 actacaccat cggaccgtac tcgacgccgg tgatgaacat cgctccgggc gaccgggttg
     2821 tggttgagac acgcgatgca tttgacggca agattctcac ggagcaggat ctgccgagtc
     2881 agaagctgcg ggtgccgttc ctgaatccgc aaagcggccc gatcatgatc gagggcgcgg
     2941 agaagggcga cgtggtggcg gtgtacatcg aatcgatgct gccacgcggc gacaatccgc
     3001 gcggcacctg ctgcctgatc ccgcgcttcg gcgcgctgac cggtacggaa tacacggcaa
     3061 cgctcaatga gccgctgccc gagatcaccc gcaagatcga cgtggacgaa gaaggcgtct
     3121 actggagcaa gcgcgtgacg ctgccgtaca agccgcatat cggcacactg agcctgtcgc
     3181 cggaaataga ctcgatcaat tcgctcacgc cggacagcca cggcggcaat atggacgtgc
     3241 ccgacatggg cccgggcagc atcacttatc tgccggtccg ctccgagggt gcacggctct
     3301 ttatcggcga cgcacatgcc tgccaaggcg acggcgaagt gtgcggcacc gctgtcgaat
     3361 actccagcac gaccacgatc cacgtcgatc tgatcaaagg ctggcacatc gaatggccgc
     3421 gcctcgaaac cgatgaactg ctgatggcga taggcagcgc gcgcccgctt gaagacgcga
     3481 cgcgcattgc gtatcgcgaa ctggtgctgt ggatggaaaa ggaatacggc ttcgaccgct
     3541 gggatgccta tatgatgctc agccagtgcg gcaaggtgcg gttgggcaat ttcgtcgacc
     3601 cgaagtactc ggtcggtgcg gcgatcagca agaagtatct cgcaccggct gcctgaggag
     3661 acgccaccat ggatctgcaa ctcaaaggtt tgaaggcact tatcaccggc ggcaccaagg
     3721 gtatcggccg cgccattgcg caaacgctgg cagccgaagg ctcggacgtc gcgctgtgcg
     3781 cacgtgatgg tgccgccgtg gaacagacgg tggccgacat cgtgcaagtc accggtggcg
     3841 cggtcaaggt gtgcggcgcc tcggtcgacg tttcgaacgg tccggcgtta caggcctgga
     3901 cccgcgaagc cgccgcgcaa ctgggcggaa tcgacatcgt ggtcgccaat gtgagcgcgc
     3961 tgtcaatcgg cagcgatctc gaatcgtggc gcaaagagtt cgaaaccgac ttgatgggta
     4021 cggtcaacct ggtcgacgcc gcgctgccgt ttctcgaagc gagcaaggcc gcctcgatca
     4081 tcgcgatatc gagcgtgtcc ggccgtgaaa tcgactttgc cgccggccct tatgggacat
     4141 tcaaggcggc gctgatccac tacatgcaag gcctcgcgta ccaactcgcg ccgaagggca
     4201 tccgggcgaa cgccgtgtcg ccaggcaacg tctatttcga aggcggtgtg tgggatcaca
     4261 tcgaacacaa cgatccgaag ctgttcgcgg aagcactcgc gttgaatccc agcggacgca
     4321 tggcgcgccc acaggagatc gcgaacgcgg tcgcgttcgt cgcgagtccg gcggcgagct
     4381 tcgtgagcgg ggcgaatctt gtagtggatg gcgcgctcac gcgcggggtg caactctaag
     4441 tgccgagaag ctgcgtcacg taggtcgatt cgacaaaaaa ccaaggagcc ttgacgataa
     4501 tgaaacgaat ctcttccctt ctgactgccg gccgctcacg cggcgcggcg gccgttctgc
     4561 tggtttccct cgcaatggcg agccacgggg ccatggccgc cgatccgatc aggatcggcg
     4621 tgcccgtggg cctgagcggc gcgaatagcg tggttgctcc gtcggtggtt caggcagcgc
     4681 aactggctgt cgacgagatc aatgcgaaag gcggcattct ggggcgtcag gtcggcctgg
     4741 aagtggccga tgacggtagc ggcgcggcgg gcgcgcaaaa ggcgttcgat tcgctgatct
     4801 tcgaaaagaa ggtcaatgcc ctcatctcga tggaaacgag tgccgcgcgc aacgggggcc
     4861 tgccgatcgt gacgcgcggc aaggtgccgt tcatctacac gtcgttctat gaaggccgct
     4921 cctgcaatcg gtacatgtac gtcgatgcgt gggtgcccga ccagcaggtc gcgccgatcg
     4981 tcgattactt catgaagcag cagaaggcga agacgttctt cctgatcggc agcgactacg
     5041 cgttcggtcg cggcatgctc gacttcacga agacc
//
LOCUS       DATASH010000643         1789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2282381,
            whole genome shotgun sequence.
ACCESSION   DATASH010000643 DATASH010000000
VERSION     DATASH010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2282381"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /locus_tag="VHV99_17415"
     CDS             complement(<1..997)
                     /locus_tag="VHV99_17415"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEX3637227.1"
                     /translation="MASHRVAGAPLDFVAIRDELEVPGDFAAPVQAEAEAAAAHPDPP
                     DAAGHADPNSPVVDATDIPFVTIDPAGSRDLDQALHIARLGDGYRVHYAIADVAAFVR
                     PGSALDAEAFRRGETLYFPDARVPLHPLVLSEGAASLLPDQVRPAVLWQIDLDANGEV
                     TAVDVHRARVRSQRQFDYETVQRLLDGPSPPDQFVLLREVGQLGLARARADHAIDLGL
                     PEQQVVADGPGAWTIRYRSPLEVEAYNAQISILTGRCAARLMLDHHVGVLRTVPAPTP
                     GAVHALQRAARALHVPWPHGAEPGDVLATLDRSRTQNITFVEHASALLRGSGYVAF"
     gene            complement(1036..1443)
                     /locus_tag="VHV99_17420"
     CDS             complement(1036..1443)
                     /locus_tag="VHV99_17420"
                     /inference="COORDINATES: protein motif:HMM:NF019028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone H1-like repetitive region-containing
                     protein"
                     /protein_id="HEX3637228.1"
                     /translation="MTAPRRPARKAAKKAAKRAPAKRTAARKAPAKRSTAAKKAPARK
                     TAAKKTAAKRTAAKKAPARKTAAKKAPARKTAAKKTAAKRTARKTTARKTTARKTTAR
                     KTTARKTTARKTAAKRAPAKRAAAKRAPAKRAR"
BASE COUNT          265 a          575 c          601 g          348 t
ORIGIN      
        1 tgaaggccac gtaacccgag ccgcgcagca acgctgaggc gtgctcgacg aacgtgatgt
       61 tctgcgtgcg tgaccgatcg agcgtggcca atacatcgcc gggctcggcg ccgtgcggcc
      121 acgggacatg caacgctcgc gcggcgcgct gcagtgcgtg taccgctccg ggtgtcggag
      181 ccgggaccgt acgcagcacg ccgacgtgat ggtcgagcat cagtcgggcc gcgcaccgac
      241 ccgtgagtat cgagatctgc gcgttgtacg cctcgacctc gagcggactg cggtatcgga
      301 tcgtccacgc ccccggcccg tccgcgacca cttgctgctc gggcagtccg aggtcaatcg
      361 catgatcggc gcgtgcgcgc gcaagaccga gctgtcccac ctcgcgcagg aggacgaact
      421 gatcaggtgg tgacggaccg tccagcagac gctgcacggt ctcgtagtcg aactgccgct
      481 gactacggac tcgggcgcga tgcacgtcga cggcggtcac ctcgccgttt gcgtcgaggt
      541 cgatctgcca caagacggcc ggccgcactt gatcaggaag caggctggcc gcgccttcgc
      601 tcagtacgag cggatgcagc ggcacgcgcg catcgggaaa gtacagcgtc tcaccgcgcc
      661 ggaatgcttc ggcatcgagc gccgagcccg gccggacgaa cgcggcaacg tcggctatcg
      721 cgtagtggac ccgatagccg tcacccagtc gggcgatgtg cagcgcctgg tcgagatcgc
      781 ggctgcctgc cgggtcgatg gtgacaaaag ggatatcggt ggcatcaacg accgggctgt
      841 tcgggtccgc gtggccggcc gcatccggtg gatcagggtg ggccgcggcc gcctcggcct
      901 cagcctgcac cggtgcagcg aaatcgccgg gcacctcaag ctcgtcacgt attgcgacga
      961 aatcgagagg tgcaccggcg actcgatgtg aagccatcag cacctaccga cagaccgcgt
     1021 cggcgcggtg ctgtctcagc gggctcgctt ggccggagcc ctcttggccg cggcgcgctt
     1081 ggccggagcc cgcttggcgg cagtcttgcg tgccgtcgtc ttgcgggccg ttgtcttgcg
     1141 cgctgtggtc ttgcgcgctg tggtcttgcg cgccgtcgtc ttgcgggcgg tgcgcttggc
     1201 cgcggtcttc ttcgctgcag tcttgcgcgc gggggccttc ttcgcggcag tcttgcgagc
     1261 aggtgccttc ttcgctgccg tgcgcttcgc tgcggtcttc ttcgctgcag tcttgcgcgc
     1321 gggtgccttc ttggccgccg tactgcgttt ggccggcgcc ttgcgagctg cggtccgctt
     1381 cgcgggagct ctcttcgcag ccttcttcgc ggcctttctg gccggccttc tcggtgcggt
     1441 catacgtgtg cctcctaggc gactgtcaac gaatgattca tccgtcaaca acaagtggac
     1501 gatgtaatgc atgcaaccgt caacacgcca cgccgttcgt gttatacgcg cgatgtcgcg
     1561 cgcacgaacg caggcacgcc gtgcgcaaca cgcctgttgc agcaactttt tttgaaaatt
     1621 tttttgagcc gtgcgcacag cgtgcaacac gtcgatgacg tcgcaggttg gacagagttt
     1681 gtgcttactg cacaagggtt ttctctgtcg acacgttgaa atgcacggcg tgcctgcgtt
     1741 cgtgtgcgca tcatcgcgcg tataacgcga acggcgtggc gtgttgacg
//
LOCUS       DATASH010000644         3315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3665161,
            whole genome shotgun sequence.
ACCESSION   DATASH010000644 DATASH010000000
VERSION     DATASH010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3665161"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="VHV99_17425"
     CDS             <1..425
                     /locus_tag="VHV99_17425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MlrC C-terminal domain-containing protein"
                     /protein_id="HEX3637229.1"
                     /translation="PFDVPARVVRLHDGKGVGRRGQLAGCSFDLGPSVALQIGGVTVI
                     VISKRHQCHEPMFFEMFGIDIASARVVALKSRGHFRAAFDEFFSGEQILSVDAPGLTS
                     PILARFDFKKLPRPVVPMDDIANWSPTVRAVQPRSVAQ"
     gene            422..1591
                     /locus_tag="VHV99_17430"
     CDS             422..1591
                     /locus_tag="VHV99_17430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HEX3637230.1"
                     /translation="MSEFCNESVGRQSLDELDTPCLLLDETRMTRNIERLRAHLGTLG
                     VQLRPHLKTPKSIEVAQRTMDSAAGPAAVSTLKEAEQFADAGVRDILYAVGIAPNKLD
                     RAGALRRGGVDLSIVVDNVEAAQAVAAKARETGDKIPTLIEIDSDGHRAGIPPQRPDD
                     LVAIGRVLHEGGAELRGVLTHAGESYSCKSLDEIVAMAERERAAAVTCAQTLRAAGLP
                     APVVSVGSTPTAHFARDLTGVTEVRAGVFVFFDLVMAGLGVCSTDDLALSVLATVIGH
                     QRDKGWILVDAGWMAMSRDRGTARQRIDQGYGIVCDVDSRIYPDLIMVEANQEQGILA
                     LRPGSAAVLPDLPVGAQVRILPNHACATGAQHGAYQVVRPGSREVLARWQRFSGW"
     gene            2042..3019
                     /locus_tag="VHV99_17435"
     CDS             2042..3019
                     /locus_tag="VHV99_17435"
                     /EC_number="4.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012339928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threo-3-hydroxy-L-aspartate ammonia-lyase"
                     /protein_id="HEX3637231.1"
                     /translation="MNRNTMFQLPTYTDVAAAAERIKGRVHRTAVVTSRIVDEEIGAK
                     VFFKCECLQRTGAFKFRGAFNALSKLDERQRRNGVVAYSSGNHAQAIALSSFVLGIPA
                     TLVMPHDAPAAKLAATEAYGGKVILYDRYTEDREAIGRALAEEHGLTLIPSYDHPDVI
                     AGQGTAAMELFEEVGDLDALFVPLGGGGLLSGSALSAAALAPNCKVYGVEPLAGNDGQ
                     QSLEAGSIVHIDTPKTIADGAQTQHLGAYTFEIIRRHVESIHTATDAQLIACMRFFAT
                     RMKMVVEPTGCLGFAALRDMRSKLQGKRVGVLVSGGNVDVERYCALLTA"
BASE COUNT          661 a          959 c         1049 g          646 t
ORIGIN      
        1 acccgttcga cgtgccggcg cgcgtggtgc ggctgcatga cggcaaaggt gtcggccggc
       61 gcggacagtt ggccggctgc agctttgacc tcggaccgtc ggttgcgctg cagatcggcg
      121 gcgtaacggt catcgtcatc agcaaacggc accagtgcca cgagccgatg ttcttcgaaa
      181 tgtttggcat cgatatcgcc agcgcgcgcg ttgtagcgtt gaagtcgcgc ggccatttca
      241 gggctgcatt cgatgaattc ttttctggcg aacaaatctt gagcgtcgat gcgccgggcc
      301 tgacttcgcc cattctcgcg cgtttcgact tcaagaaatt accgaggccc gtcgtaccca
      361 tggacgacat tgcgaattgg agcccgacag tccgtgctgt tcagccccgg agcgtcgcgc
      421 aatgagtgaa ttttgcaatg aaagcgtcgg ccgtcagtcg ctggacgaac tggacacacc
      481 gtgtctgttg ctggatgaaa cgcggatgac gcgcaacatc gagcggctgc gcgcacacct
      541 cggaacgttg ggcgtgcagc ttcggccgca tctgaagacg ccgaaatcga tcgaggtcgc
      601 gcaacgcacg atggacagcg cagcgggtcc cgcggcagtc tcgacgctca aggaggcgga
      661 gcagttcgcc gatgcgggcg tgcgcgacat tctctacgct gtcggcatcg cgccgaacaa
      721 gctcgaccgc gccggcgcgc ttaggcgcgg cggcgtcgac ttgtcgatcg tcgtggataa
      781 cgtcgaggcc gcgcaggcgg tggcggcaaa ggcgcgcgag accggcgaca agattccgac
      841 gctaatcgaa atagatagtg atggccaccg cgctggcata ccgccgcaac gtcccgacga
      901 tctcgttgca atcggccgcg tgttgcacga gggcggtgct gagttgcggg gcgtcctcac
      961 gcatgcgggc gagtcctaca gttgcaaaag cctcgacgaa atagtggcga tggctgaacg
     1021 cgagcgcgcg gcggcggtaa cgtgtgcgca gactctgcgt gccgcggggc tgccggctcc
     1081 ggtcgtcagc gtcggctcga cgccgaccgc acactttgcg cgcgacctga ccggcgtgac
     1141 ggaagtacgc gcaggcgtat tcgtgttctt cgatctcgtc atggccggcc tcggcgtgtg
     1201 ctcgaccgac gacctcgcgc tcagcgtgct tgcgaccgtt atcggtcatc agcgcgataa
     1261 gggctggata ctcgttgacg cgggctggat ggcaatgtcg cgcgatcgag gtaccgcccg
     1321 gcagcgcatc gaccaggggt acggcattgt ctgcgatgtc gattcccgga tatatccgga
     1381 tctgatcatg gtcgaagcca accaggagca gggaattctt gcgctaaggc cgggcagcgc
     1441 agccgtgctg cccgatctgc cggtcggcgc tcaggtgcgg atattgccga atcacgcgtg
     1501 cgcaaccggc gcgcaacatg gggcatatca ggtcgtacgc cccggttcgc gcgaggtgct
     1561 cgcgcgctgg caacgattca gcggatggta gacggaaagg atcggtaccg aacggggcat
     1621 ggaacgtgcc gcgttcactc cgtgtgcgca gtccacaccg aattcgtcgc accagcaccg
     1681 gactgtttca tccccgaagg ggcgctccct tcggggcaca gctgaccggt cgctgtgctc
     1741 gaaccgcaac gcttcgatgt cgcgtgcatt tgataaatgc agccagaaag cgatgggatg
     1801 acgaagtggt tcgagttcca ttgcatcact tagacgacgg cccgagcgaa ggagctgtca
     1861 aagaaagttt attgacagat tgacgaaaaa aataacaata gataatctgt ctaaatgttg
     1921 aaatgtggat gtagcagtag ccacgggtgc gatctgttcg tgtctgtcga ctcgctgtgt
     1981 gaacgtccac caggatttgc acggtccccc gccccaagtg tgctgtcaag ctataaagcg
     2041 gatgaatagg aatactatgt ttcagcttcc cacttataca gatgtcgctg cggccgccga
     2101 gcgcatcaag gggcgagtgc acaggaccgc agttgtgacc tcgcgcatcg tagacgaaga
     2161 aataggcgcg aaggttttct tcaagtgcga atgtcttcaa cgcacgggtg ccttcaagtt
     2221 ccgaggcgcc ttcaacgcgc tgtcgaagct ggacgagcgc caacgacgga atggggtggt
     2281 cgcctattca tccggcaacc atgcgcaagc catcgcgcta tcgagtttcg tgctgggcat
     2341 tcccgccacg ctcgtcatgc cgcacgatgc cccagccgca aagcttgccg cgacggaagc
     2401 ttatggcggc aaggtgatct tgtacgacag atacacggaa gaccgggaag caatcgggcg
     2461 ggcgctcgcc gaagagcatg ggctgacgct gatcccatcg tacgatcatc cagacgtgat
     2521 cgccggtcaa ggcaccgcag caatggagtt gttcgaggag gtgggcgatc tcgacgcctt
     2581 gttcgtgcct ctgggcggcg gcggtttgct gtcaggctct gcgctctccg cggctgccct
     2641 ggctccgaat tgcaaagtct acggtgttga gccattggct ggcaacgacg ggcagcaatc
     2701 gctcgaagcg ggttccattg tgcatatcga cacgccgaaa accattgccg atggcgctca
     2761 gactcagcac ctcggggctt acacgttcga gattatccgc cgccacgtcg aaagcataca
     2821 cacggcaacg gatgcgcaat tgatcgcgtg catgcgcttt tttgcaactc gcatgaagat
     2881 ggtggtcgag ccgactggct gccttggttt cgccgctctg cgcgatatgc ggtcgaagtt
     2941 gcaaggaaag cgtgtcggcg tactcgtcag tggcggcaac gtcgatgtgg agcgctactg
     3001 cgccttattg acggcttagc cagacacgaa ggaactgata ttgaggcgat gcccgggcgc
     3061 gcagggcgga cggatgctgc gctcggtcaa gtcatcgctc aaccgcttta agttgtgcac
     3121 cacaagcgcc cgcgttgccc ctcgacatcc ggtcggtaac gacggctgaa agcagtgggc
     3181 ccgtcaccca cagttaatgg ccagaccggc actgttctta tgactcttcg acactgacat
     3241 gaaaaatact cgtgtatttc caacagcctc aatcccttcg gccggcgagt ttgagtccag
     3301 ccacggacaa atgag
//
LOCUS       DATASH010000645         6617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6433824,
            whole genome shotgun sequence.
ACCESSION   DATASH010000645 DATASH010000000
VERSION     DATASH010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6617
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6433824"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>6617
                     /locus_tag="VHV99_17440"
     CDS             <1..>6617
                     /locus_tag="VHV99_17440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3637232.1"
                     /translation="DDVAGFDAAFFGISPREAQQMDPQQRLLLELAWETFEDAGVRPR
                     DMEGSNCAVYIGVASPDYGNRFVDDLNAVDPYSATGNTLSIASNRLSYLFDLRGPSVS
                     VDTACSSSLVALHQACQALQSGDAEMALAGGVNLLLHPFGFVTFSKASMLSPRGRCRA
                     FDATGDGYVRSEGGGLVMLKTLDRALADGDTIHAVIAGSGVNSDGYSQGGISVPGAAT
                     QAALLRTVYERAGVDPRSLAYVEAHGTGTAVGDPIEARALMEVASAGRPVDQPLLIGS
                     VKTNVGHLETASGMAGLMKAILCLKHRAVPKTLHFETPNPAIDFVGGRLRVVDRLTSL
                     ESGTEPLVIGVNSFGFGGTNAHVVLREAPVAGAAARAENDSKALPPLILSTRTAAALP
                     ALAARYLSMFDNGTPWSTLASNAAHRRQWLKHRAIIAASTPAEARAALAALAAPTPDQ
                     TEPALVQGETPADDTRLALVFSGNGSQWAGMGKQLLEQEPVFRAALDELDALWRADGS
                     ASLVEMLREGVTAATLEATEHAQPLLFAIQVGVVRVIEARGVGYDVCLGHSVGEVAAA
                     WASGALTLPQAVHVIKIRSRAQASTRGTGRMAAAGLGEAAMRALMAQLGVDAAVEVAG
                     VNSPQAVTLAGPLGALQVLEAAVKESGRFFQMLELDYAFHSRQMDPIEPSVLQGLADL
                     TPNTATRSFVSTVTGAELTGTELGAHYWWRNIREPVRFGDAVAQVAQTGVRLFLEVGP
                     HSILRTYVTQTLDDVRITGRSLPTLKRNQDSAQMLHHAIYAMLANGARVDIERFAPLS
                     ARVALPSYPWQHERYWLGPTAEAYNLVNRRREHPLLGYRLREHALAWENQLDPAGLPM
                     LADHVVDGGVAFPGAGYVEMALAAARVHFGTQSCAVENLEIRLPVVFQPQHSKLFRFT
                     VDVRTASFTIETRDRMSDEAWSLNVTGRLLASGCALHEAGDASTLAATTLTELFAQPA
                     MSGTTLYANTTAIGLSYGPAFQWVRTVHVNAPAGMALAEVEVPAVLVQSGTESAAWLS
                     AYAFHPALMDSGFHPLFALLASADQTEIEHAAYVPVQIGRIDYLRGDAVSYVVARIER
                     RSPHSVVASFEFADEHGAIVARLSACRFRRVDLMGRRQHAPARYAYIAEAKPRAADLD
                     ARMLPQPATLLEEAAATLAAQEDQPRRQAHLTEMLPLLDVLASAYALNALDAMNVFAQ
                     ASLPDCAHPALLARLAQIVVEDGLAQRDGTRLVRDSAACETLPGIDELWRDLLAQSPA
                     HVAELTLLAHCGAALPAVLRGEVSGAQILSPSRSSLVEHFFEASPTWAHVNALSSACL
                     HRAIEGWSEARRLRVLELDSPSSEVLQPLAIHMPVSRCDYTIAGTHEQLSGFDASEHP
                     SIRIANIEFGDAPRLTGTEAGERYDMVVASHVLAAQTEPRALLAALRGWLAPGALVVI
                     AEPRNNRFADIVFDLDADAAHANARRAAWLSPAALTKHLEEAGFEQITRHREQGLDLE
                     GAPTLIVAREPYPGASAGNETEARTPSAHWSLLYAAQAGADETLAAALQAAGHTTSST
                     TLTALHHDMSKLAAPAGRAHHVVFIAPDHSLSADADGAEVMHVQQQTTLALAQLVRDL
                     AAVAGAMQPQLWIVTRGGAPVVTPFADAAMLRPEQAAMWGLGRVLANEHPELSCRLID
                     VSPACRHAGAELARELLASDGEEEVLLTSHGRYVPRMLPAASAAARRDSAAAVRPRNE
                     AAVLGFASPGSLRNLEWFAPPQRELAADEVEIEPVATGLNFRDVMYAMGLLSDEAVEN
                     GFAGATIGMELSGRVARVGRDVRRFAPGDAVLGFAPASFASHVRTKAEAIAHKPERLT
                     FEEAATVPTTFFTAYYALCELARLRRGERVLVHGAAGGVGIAAIQLARHLGAEVFATA
                     GSREKREFVRLLGADHVFDSRSLAFADEIRERTQGAGVDIVLNSLAGEAMVRSIDTLR
                     PFGRFLELGKRDFYENSRIGLRPFRNNISYFGIDADQLMSALPDLTARLFEEVMQLFT
                     AGMLHPLPYRAFPAERVEEAFRHMQQARQIGKILVTYPAGTPSPTRAKSDAEALQLDP
                     AAAYLIAGGTGGLGFATARWMMSRGARHLTLASRSGLLAPELAAEAARWREENGTQVH
                     TAACDVTDAAALDALLAEIAARGTPLKGVLHSAMVIDDGLVRNLDDARFAAVLAPKLA
                     GAWNLHRATRDATLD"
BASE COUNT         1053 a         2204 c         2243 g         1117 t
ORIGIN      
        1 gacgacgtcg ccggtttcga cgcggccttc ttcggcattt cgccgcgcga agcacagcag
       61 atggatccgc agcagcgcct gttgctcgaa ctcgcatggg aaaccttcga ggatgcgggc
      121 gtgcgtccgc gcgacatgga aggcagcaat tgcgcggtct acatcggcgt cgcgagcccg
      181 gattacggca atcgtttcgt cgacgacctg aacgcggtcg atccttattc ggcaaccggc
      241 aacacgctca gcatcgcgtc caaccgcctg tcctatctgt tcgatctgcg cgggccgagc
      301 gtgtcggtgg acacggcgtg ctcgtcgtcg ctggtcgcct tgcatcaggc atgtcaggca
      361 ctgcaatcgg gcgacgctga gatggcgctc gcaggcggcg tcaatctgct gctgcatcca
      421 ttcggcttcg taacgttctc gaaggcatcc atgttgtcgc cgcgcggacg ctgccgcgcg
      481 tttgacgcca ccggcgacgg ttacgtccgc tccgaaggcg gcggcctggt gatgctcaag
      541 acgctcgacc gtgcgttggc cgacggcgac acgatccacg ccgtgatcgc cggttcgggc
      601 gtcaactcgg acggctactc gcaaggcggt atcagcgtgc cgggcgcggc gacgcaggca
      661 gctttgctgc gcacagtcta cgaacgcgcg ggcgtcgatc cgcgttcgct cgcctatgtc
      721 gaggcgcacg gcacgggcac ggcggtcggc gatccgatcg aagcgcgcgc cttgatggaa
      781 gtcgcgtcgg cgggccggcc ggtcgatcag cctttgttga tcggctccgt gaagaccaac
      841 gtgggtcacc tcgaaacggc ttcgggcatg gccggtttga tgaaggcgat cctgtgcctg
      901 aagcaccgcg cggtgccgaa aaccctgcac ttcgaaacac cgaatccggc gatcgatttc
      961 gtcggtggcc gcttgcgtgt cgtggatcgg ctgacgtcgc tcgagagtgg cacggaaccg
     1021 ttggtgatcg gcgtcaattc gttcggcttc ggcggcacga atgcacacgt cgtcttgcga
     1081 gaggcgccgg tcgcaggtgc cgccgcaaga gctgaaaacg actcgaaggc actgccgccg
     1141 ctgatcctct cgacccgcac cgcagcggcg ctacccgcgc tggccgcacg ctatctgtcg
     1201 atgttcgaca acggcacgcc gtggagcacc ctggcatcga acgcggcgca tcgccgccag
     1261 tggctcaagc atcgcgcgat catcgccgcg tcgacgccgg ccgaagcgcg cgcggcgctc
     1321 gccgcgcttg ccgcgccgac gccggatcag acagaaccgg ccctcgtgca gggcgaaacc
     1381 ccggccgacg atacacgtct cgcgctggtg ttctccggca acggctcgca gtgggccggc
     1441 atgggcaagc aattgctcga acaggagccg gtgttccgtg ccgcgctcga cgaactcgac
     1501 gcgctgtggc gcgccgacgg cagtgcctcg ctggtcgaga tgctgcgcga aggcgtcacc
     1561 gccgcgacac tggaagcgac cgagcacgcg caaccgctgc tgttcgcgat ccaggtgggc
     1621 gtggtgcgcg tcatcgaagc gcgtggtgtc ggctatgacg tgtgcctcgg ccacagcgtc
     1681 ggtgaagtcg cggcggcatg ggcgtcgggt gctttgacac tcccgcaagc cgtccacgtg
     1741 atcaagattc gcagccgcgc tcaggcaagc acgcgcggca cgggccgcat ggctgccgcc
     1801 ggcctcggcg aagccgccat gcgtgcactg atggcgcaac tcggcgtgga cgccgcggtg
     1861 gaagtcgcgg gtgtcaacag cccgcaggcg gtcacgctgg ccggcccgct cggcgcgttg
     1921 caggtgctcg aagccgcggt gaaggagagc ggccgcttct tccagatgct ggaactggac
     1981 tacgcgttcc acagccgtca gatggacccg atcgagccga gcgtgctgca gggtctcgcg
     2041 gatctcacgc cgaatacggc aacgcgtagc ttcgtctcga cagtgacagg cgccgaactc
     2101 actggcaccg aactcggcgc gcactactgg tggcgcaata tccgcgagcc ggtgcgtttc
     2161 ggcgatgccg tcgctcaggt cgcccagacg ggcgtgcgcc tgttcctcga agtcggcccg
     2221 cattcgattc tgcgcaccta tgtgacgcag acgctcgacg acgtgcgcat caccggccgc
     2281 tcgctgccga cgctcaagcg caaccaggac agcgcgcaga tgctgcacca cgcgatctac
     2341 gcgatgctgg cgaacggcgc gcgcgtcgat atcgaacgct tcgcgccgtt gagcgcacgc
     2401 gtggcgttgc cgtcctatcc gtggcaacac gaacgctact ggctcggccc gaccgccgag
     2461 gcctataacc tggtcaatcg ccgccgcgaa catccgctgc tcggttaccg tctgcgcgag
     2521 cacgcgctcg cctgggaaaa tcagcttgat ccagccggtc tgccgatgct ggccgatcac
     2581 gtggtcgacg gcggcgtggc gttccccgga gccggctacg tcgaaatggc gctggccgcc
     2641 gcgcgcgtgc atttcggcac gcaaagctgc gctgtcgaga acctcgaaat ccgtctgccg
     2701 gtggtgttcc agccgcagca ttcgaagctg ttccgcttca ccgtcgatgt gcgcaccgca
     2761 agcttcacga tcgaaacacg cgaccggatg tccgacgaag cgtggtcgct caacgtcacc
     2821 ggccgcctgc tggcgagcgg ctgcgcgctg cacgaggccg gcgacgcgag cacgctggcg
     2881 gccactacgc tcaccgagct gttcgcacag ccggctatgt ccggcacaac cttgtatgcg
     2941 aacaccaccg cgatcggttt gagctacggc ccggcattcc agtgggtgcg cacggtgcac
     3001 gtcaacgcgc ccgccggcat ggcgctcgcg gaagtggaag tccccgctgt cctcgtgcag
     3061 agcggaaccg aatccgcagc ctggctgagc gcctatgcgt ttcatccggc cttgatggat
     3121 agcggtttcc atccgctgtt cgccttgctc gcgtcggcgg atcagacaga gatcgaacac
     3181 gcggcctatg tgccggtgca aatcgggcgc atcgactatc tgcgcggcga cgcggtcagc
     3241 tacgtagtcg cccggatcga gcggcgcagt ccgcactccg tggtggcatc gttcgaattc
     3301 gccgacgagc acggcgcgat cgtcgcgcgg ctcagcgcgt gccgtttccg ccgcgtcgat
     3361 ctgatggggc gccgtcagca tgcgccggca cgctacgcgt atatcgccga agcgaaaccg
     3421 cgcgccgccg acctcgacgc acgcatgttg cctcagccgg ccacgttgct ggaagaagcc
     3481 gccgcgacat tggccgcgca agaagatcag ccgcgcagac aggcgcatct gaccgaaatg
     3541 ctgccgctgc tcgacgtgct cgcgagcgct tacgcattga acgcgctcga cgcgatgaac
     3601 gtttttgcgc aagcgtcgct gccggactgt gcgcatcccg cactgctggc gcgtctcgcg
     3661 cagatcgtcg tggaagacgg cctggcgcag cgtgacggta cgcgtctcgt gcgcgacagc
     3721 gcggcctgcg agactctgcc gggcatcgac gaactgtggc gcgacctgct tgcgcagtcg
     3781 cccgcgcatg tggccgaact gacgctgctc gcgcactgtg gcgcggcgct gcctgccgta
     3841 ttgcgcggcg aggtgagcgg cgcgcagatc ctgtcgccgt cgcgcagcag tctggtcgag
     3901 catttcttcg aagcttcgcc gacatgggcg catgtcaatg cattgagcag cgcctgcctg
     3961 catcgggcga tcgaagggtg gagcgaagcg cgccgcttgc gcgtgctcga actcgattcg
     4021 ccgtcgagcg aggtgcttca gccgctcgcg attcacatgc cggtgtcgcg ctgcgattac
     4081 acgattgcag gcacgcatga acagttgagc ggtttcgacg cgagcgagca tccctccatc
     4141 cggatcgcaa acatcgaatt cggtgacgcg ccacgcctca ccggtaccga ggctggcgag
     4201 cgctacgaca tggtggtggc gagccacgtg ctggccgcac aaactgagcc gcgtgcgctg
     4261 ctcgccgcgc tgcgcggctg gctcgcgccg ggcgcgctgg tcgtgatcgc ggagccgcgc
     4321 aataaccgct tcgccgatat cgtgttcgat ctcgacgcgg atgccgcgca cgccaacgcg
     4381 cgccgtgcgg catggttgtc gccggcagcg ttgacgaaac acctcgagga agcgggtttc
     4441 gaacagatca cgcggcatag ggagcaaggc ctggatctcg aaggcgcgcc gacgctgatc
     4501 gtcgcgcgcg agccgtatcc gggcgcttct gccggcaatg aaaccgaagc gcggacgccg
     4561 tctgcgcatt ggtccttgct gtacgcagcg caagccggcg ccgacgagac cctggctgcg
     4621 gccttgcagg ccgccggcca cacgacgtcg agcaccacgc tcacggcgtt gcatcacgac
     4681 atgagcaagc tggccgcgcc ggcagggcgt gcgcatcacg tcgtattcat cgcgccggat
     4741 cacagcttgt ccgccgatgc ggacggcgcg gaagtgatgc acgtccagca gcagaccacg
     4801 ctcgcgctgg cgcaactcgt gcgcgatctc gccgcggttg ccggcgcgat gcagccgcaa
     4861 ttgtggatcg tgacgcgcgg cggtgcgccg gtcgtcacac cgtttgccga tgcggcgatg
     4921 ctgcgtcccg agcaggccgc gatgtggggg cttggccggg tgctggccaa cgagcatccg
     4981 gaattgtcgt gccgtttgat cgatgtatcg ccggcgtgcc gccatgcagg cgccgagttg
     5041 gcgcgcgaac tgctcgcatc cgacggcgag gaagaagtcc tgctcacgtc tcacggccgc
     5101 tatgtgccgc gcatgctgcc ggcggctagc gcggcagcgc gccgcgattc ggctgccgcg
     5161 gtacgtccgc gcaacgaagc ggcggtgctc ggttttgcgt cgcccggctc gttgcgcaat
     5221 cttgaatggt tcgcgccgcc gcaacgtgaa ctcgccgcgg acgaagtgga aatcgaaccg
     5281 gttgcaaccg gtctgaattt ccgcgacgtg atgtacgcaa tgggcctgct gtccgacgag
     5341 gccgtcgaaa acggctttgc cggcgcgacg atcggcatgg agctgtcggg gcgcgtcgcg
     5401 cgcgtcggcc gggacgtgcg gcgtttcgca ccgggcgatg cggtactcgg tttcgcgccg
     5461 gcttctttcg cgagccacgt gcgcacgaag gccgaggcca tcgctcacaa gcccgaacgt
     5521 ctgacgttcg aagaagccgc cacggtgccg accaccttct tcacggcgta ctacgcgctg
     5581 tgcgagctgg cgcgactgcg ccgcggcgag cgcgtgctgg tgcatggcgc ggcgggcggc
     5641 gtgggcatcg cggcgatcca gctcgcgcgc cacctgggtg cggaggtgtt tgccacggcg
     5701 ggcagccgcg agaagcgcga attcgtgcgt ctgctcggcg cggatcacgt gttcgattcg
     5761 cgcagcctgg cgtttgccga tgagattcgc gagcgcacgc aaggggcggg cgtcgatatc
     5821 gtgctcaatt cgctcgccgg tgaagcgatg gtgcgcagca tcgacacgct gcgtccgttc
     5881 ggccgttttc tcgaactcgg caagcgcgac ttctacgaga acagccgcat cggcttgcgg
     5941 ccgttccgca ataacatcag ctatttcggc atcgatgccg accagttgat gagtgcattg
     6001 ccggatttga ccgcgcgtct gttcgaagag gtcatgcaac tgttcacggc aggcatgctg
     6061 catccgttgc cgtatcgcgc gttcccggct gagcgcgtcg aggaagcatt ccgtcacatg
     6121 cagcaggcgc gtcagatcgg caagatcctc gtgacgtatc cggcaggcac gccgagtcca
     6181 acgcgcgcga agtccgacgc tgaggcgctg caactcgatc cggcggcggc gtatctgatc
     6241 gccggcggca cgggtgggct cggtttcgcc accgcgcgct ggatgatgtc gcgcggcgcg
     6301 cggcatctga cgctcgcgag ccgttcaggc ttgctcgccc cggagctcgc tgccgaggcc
     6361 gcgcgttggc gcgaggaaaa cggcacgcag gtgcacaccg cagcctgcga tgtcacggac
     6421 gcagccgcac tcgacgcact gctcgccgag atcgccgcac gcggcacgcc gctcaagggc
     6481 gtgctccatt cggcgatggt gatcgacgac ggcctcgtgc gcaatctcga cgacgcgcgt
     6541 ttcgccgcgg tgctcgcacc gaagctggcc ggcgcatgga acctgcaccg cgccacgcgc
     6601 gacgcgacac tcgattt
//
LOCUS       DATASH010000646         4230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5842215,
            whole genome shotgun sequence.
ACCESSION   DATASH010000646 DATASH010000000
VERSION     DATASH010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4230
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5842215"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VHV99_17445"
     CDS             complement(<1..546)
                     /locus_tag="VHV99_17445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HEX3637233.1"
                     /translation="MKHIVKHIHFVGIGGVGMSGIAEVLVNLGYQVSGSDLSSNAITD
                     RLAALGARIAIGHAAENIEGANAVVVSTAVRSDNPEVLAARHRRIPIVPRAVMLAELM
                     RLKQGIAIAGTHGKTTTTSLVASVLAAGGLDPTFVIGGRLISAGANARLGTGDFIVAE
                     ADESDASFLNLFPVIEVITNID"
     gene            complement(543..1661)
                     /gene="murG"
                     /locus_tag="VHV99_17450"
     CDS             complement(543..1661)
                     /gene="murG"
                     /locus_tag="VHV99_17450"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEX3637234.1"
                     /translation="MTALPQRTLMVMAGGTGGHVFPGLAVAHLMQAWGWKVVWLGNPA
                     GMEATLVPKHGIPMEYVRFGGLRGKGLKTKLMLPLNLLRACSQSLSVLRRVKPDVVLG
                     MGGYITFPAGLMTALSGRPLVLHEQNSIAGLANKVLAKLAKRVLVAFPNALPHGEWTG
                     NPIRAELARAIAPKARYAQRSGPLNVLVVGGSLGAAALNEVVPRAVAQLAPNERPRIV
                     HQAGAKHIEALRENYAAAGLKAGADVELVPFIDDMTSAYANADLVICRSGAMTVSEIS
                     AVGVAAFFVPFPFAVDDHQTTNAAFLADNGAALVVQQRDLSAETLADWLRSQTRETLA
                     EMAERSRSLAKPDATEQVAQICATVAGSISGASPEGKQ"
     gene            complement(1774..3048)
                     /gene="ftsW"
                     /locus_tag="VHV99_17455"
     CDS             complement(1774..3048)
                     /gene="ftsW"
                     /locus_tag="VHV99_17455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEX3637235.1"
                     /translation="MSWSERFGSSLAGAGNGDTGGASRASSRTGGSGLASAVNGVRPL
                     RSRMLDYDHSLLWVVVALLGLGVVMVYSASIAMPDSPKYASYRDYAFLVRQLIFVVMG
                     SVVGVVSFRIPISTWDKYAPKLFLISLVALVIVLIPHVGKGVNGARRWIPLGITNMQP
                     SEIMKLAVTIYAANYTVRKQEYMHSFAKGFLPMAVAVGLVGALLLLEPDMGAFMVIAA
                     IAMGVLFLGGVNGKLFGGLVATAVGTFSLLVWASPWRRERIFAYLDPWDDRYAQGKAY
                     QLTHSLIAFGRGEWFGVGLGGSVEKLNYLPEAHTDFILAVIGEELGFVGVLVVILMFY
                     WIVRRSFEIGRQALALDRTFAGLVAKGIGIWFGAQTFINMGVNLGLLPTKGLTLPLVS
                     YGGSGILLNCLAVAVLMRVDYENRVLMRGGKV"
     gene            complement(3045..>4230)
                     /gene="murD"
                     /locus_tag="VHV99_17460"
     CDS             complement(3045..>4230)
                     /gene="murD"
                     /locus_tag="VHV99_17460"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015601979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX3637236.1"
                     /translation="FAQALKTLGESGYAPKVIAITGTNGKTTTTSLTGLLCERAGKKV
                     AVAGNISPAVLDKLSEAIDNTALPDVWVLELSSFQLETAHTFAPDAAVVLNITQDHLD
                     WHGGLDAYAAAKGRIFGAQTVRVLNRDDARVMALAPSRESDAAMITFGVTEPKNDGDY
                     GLLRDNGMVWLVEAHDRDASDEPAPTRRRKNEVAAPKNIALKRLMPADALRIRGLHNA
                     ANALAAYALARAIGLPGAPLLHGLREYRGEPHRVELIASIEGVDYVDDSKGTNVGATV
                     AALDGLAQRAVLIAGGDGKGQDFEPLAAPVMRWCRAVMLIGRDAPRIRAALQDTGIAM
                     TDHATLEEATRSATALAQPGDAVLLSPACASFDMFKGYAHRAAVFRNTVEDIAAERGT
                     MI"
BASE COUNT          772 a         1486 c         1255 g          717 t
ORIGIN      
        1 gtcgatgttc gtgatgactt cgatcaccgg gaacaggttc aggaacgacg catccgactc
       61 gtccgcttcg gcgacgatga agtcgcccgt acccagacgc gcattcgcgc ccgcgctgat
      121 caggcggccg ccaatcacga aggtcggatc gagcccgccc gcggccagca cgctcgccac
      181 caacgacgtg gtcgtggtct tgccgtgcgt acccgcaatc gcgataccct gcttcagccg
      241 catcagctcc gcgagcatca ccgcgcgcgg cacgatcgga atgcggcgat gacgcgcggc
      301 cagcacttcc gggttgtcgc tacgcaccgc cgtggagacg accaccgcgt tcgcgccttc
      361 aatgttctcc gccgcgtgac cgatcgcgat ccgcgcgccg agcgcagcga gacgatcggt
      421 gatcgcgttg ctcgacaggt ccgagccgct cacctgatag ccgaggttga ccagcacctc
      481 cgcgatgccg ctcatgccga caccgccgat gccgacaaag tgaatgtgtt tgacgatgtg
      541 tttcattgct ttccttctgg gctcgcgccc gaaatcgaac ccgccacagt cgcgcaaatc
      601 tgcgcgacct gttccgtggc gtcgggtttc gcgagcgagc gcgaacgctc cgccatctcc
      661 gcgagggtct ctcgcgtctg gctgcgcaac cagtcggcga gcgtttccgc cgacagatcg
      721 cgttgctgca cgaccagcgc cgcgccgtta tcggcgagga acgctgcatt agtggtttgg
      781 tgatcgtcta ctgcaaatgg gaacggcacg aagaacgccg ccacccctac tgctgaaatc
      841 tccgacacgg tcattgcgcc cgaccggcag atcaccagat ccgcattcgc gtacgcactc
      901 gtcatgtcgt cgatgaaggg cacgagctct acatcggcac cagccttcag gcctgctgcc
      961 gcgtagttct cacgcagcgc ttcaatatgc ttcgcgcccg cctgatgcac gatgcgcggc
     1021 cgttcgttcg gcgccagctg tgcaaccgcg cgcggcacca cttcattcaa cgccgccgca
     1081 cccagactgc cgcccaccac cagcacattc aacggaccgc tgcgctgcgc gtagcgtgct
     1141 ttgggtgcaa ttgcgcgcgc aagttccgca cgaatcgggt tgccggtcca ttcgccgtgc
     1201 ggcagcgcat tcgggaacgc aaccagcacc cgtttggcga gtttcgcgag caccttgttg
     1261 gcgagacccg caatcgagtt ctgttcatgc agcaccagcg ggcggccact caacgccgtc
     1321 atcaaacccg ccgggaacgt gatgtagccg cccatgccga gcacgacgtc cggcttcacg
     1381 cgacgcaaca cgctcaggct ctgcgagcag gcgcgcagca ggttcagcgg cagcatcagc
     1441 ttggtcttca gacccttgcc gcgcagcccg ccgaaacgca cgtattccat cgggatgccg
     1501 tgcttcggca ccagcgtggc ttccatgccc gcggggttgc caagccatac gaccttccag
     1561 ccccaggcct gcatcaggtg cgcgaccgcg agccccggga acacatgtcc cccggtgccg
     1621 ccggccatca ccatcagcgt gcgttgcggc agagcggtca taccttccct ccccgcatca
     1681 gcatccgttt ttggcaccgg ccgctgcgct taacttcgcg cgcttcgcgc acgaagttag
     1741 ccttcaccga aatcgtgcgc tgctgcatgg cggtcatacc ttccccccgc gcatcagcac
     1801 acgattttca taatccaccc gcatcaacac cgccaccgcc aggcagttca gcaaaatgcc
     1861 cgagccgccg tagctaacga gcggcaacgt caaacccttg gtcggcagga gcccgaggtt
     1921 cacgcccatg ttgataaacg tctgcgcgcc gaaccagatg ccgatgccct tcgccaccag
     1981 tcccgcgaac gtgcggtcga gtgcgagcgc ctgacggccg atctcgaacg agcggcgcac
     2041 gatccagtag aacatcagga tcacgacgag cacgccgaca aaacccagtt cctcgccaat
     2101 caccgcgagg atgaagtcgg tatgcgcttc cggcaaatag ttgagcttct cgacactgcc
     2161 gcccagaccg acgccgaacc actcgccgcg tccgaaagcg atcagcgagt gagtcaattg
     2221 ataagccttg ccctgcgcgt aacggtcgtc ccacgggtcg aggtaggcga agatccgctc
     2281 acgacgccac ggcgacgccc acaccagcaa gctgaaggtg ccgaccgcgg tggccaccag
     2341 cccgccgaac agcttgccgt tcacgccgcc cagaaacagc acgcccatcg cgatcgcggc
     2401 gatcaccatg aatgcgccca tgtccggctc gagcagcagc aacgcgccga cgagacccac
     2461 cgcaaccgcc atcggcagaa agcccttggc gaagctgtgc atgtattcct gcttgcgcac
     2521 cgtgtagttc gccgcgtaaa tcgtcacggc gagtttcatg atttccgacg gctgcatgtt
     2581 cgtgatgcca agcggaatcc agcggcgcgc gccgttcacg cccttgccga cgtgtggaat
     2641 cagcacgatc accagggcga ccagcgaaat caggaagagc ttcggcgcgt acttatccca
     2701 ggtcgagatc ggaatgcgga acgacacgac gccgactacc gaccccatca ccacgaaaat
     2761 gagctggcgc acgaggaatg cgtaatcgcg gtacgacgcg tacttcggtg aatcaggcat
     2821 cgcgatcgat gccgaataca ccatcacgac gccgaggccg agcagcgcga ccaccaccca
     2881 cagcagcgag tgatcgtagt cgagcatccg cgagcgcagc ggacgcacgc cgttgacagc
     2941 gctcgcgagt ccactgccgc cggtgcggct ggaagcgcgg cttgcgccgc ccgtatcgcc
     3001 attgccggca ccggcaaggc tcgaaccgaa gcgttctgac cagctcatat catcgtcccc
     3061 cgttcggccg cgatgtcttc cactgtgttg cggaacaccg ccgcgcgatg tgcataaccc
     3121 ttaaacatgt cgaagctcgc gcaagccggc gacagcagca cggcgtcccc cggttgcgcc
     3181 aatgcggtgg cggaccgcgt cgcttcttcg agcgttgcgt ggtcggtcat cgcgatgccc
     3241 gtgtcttgca acgcggcgcg aatccgcggc gcgtcgcggc cgatcagcat cacggcgcgg
     3301 caccagcgca tcacgggcgc ggcgagcggt tcgaaatcct ggcccttgcc gtcgccgccg
     3361 gcgatcagca ccgcgcgttg cgcgaggccg tcgagcgcgg caactgtcgc gcccacattg
     3421 gtgcctttgc tgtcgtcgac gtaatcgaca ccttcgatcg aggcgatcaa ttccacgcga
     3481 tgcggctcgc cgcggtattc gcgcaggccg tgcaaaagcg gcgcgccagg cagaccgatc
     3541 gcgcgcgcca gcgcatacgc ggccaaggcg tttgcggcgt tgtgcagacc gcgaatgcgc
     3601 agcgcgtcgg ccggcatcaa acgtttgagc gcgatgttct tcggcgccgc cacttcattc
     3661 ttgcgacggc gtgtcggcgc cggctcgtcg ctggcgtcgc ggtcgtgcgc ttcgaccagc
     3721 cagaccatgc cgttgtcgcg cagcagaccg tagtcgccgt cgttcttcgg ctcggtgacg
     3781 ccgaacgtga tcatcgcagc gtcactttcc ctggaaggcg cgagcgccat cacccgagcg
     3841 tcgtcacgat tcagcacgcg cacagtctgc gcaccgaaga tgcggccctt cgcagcggcg
     3901 taggcatcga ggccgccatg ccagtccaga tgatcctgcg tgatgttcaa cacgaccgcc
     3961 gcatccggcg caaacgtgtg cgcggtttcc agctggaagc tcgacagctc cagcacccac
     4021 acatcgggca acgcggtgtt gtcgatcgct tcgctcagct tgtccagcac ggccgggctg
     4081 atgttgcccg ccaccgcgac cttcttgccg gcgcgctcgc acaggagacc ggtcaggctc
     4141 gtggtcgtgg tcttgccgtt ggtgccggtg atcgcgatca ccttcggcgc gtagccgctt
     4201 tcgccgagcg tcttcaacgc ttgagcgaag
//
LOCUS       DATASH010000647         2600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6434157,
            whole genome shotgun sequence.
ACCESSION   DATASH010000647 DATASH010000000
VERSION     DATASH010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6434157"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VHV99_17465"
     CDS             <1..273
                     /locus_tag="VHV99_17465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX3637237.1"
                     /translation="PPLRSALWVVLPAAAPTIVTGMRISMGIAWLVIVAAEMLVGGTG
                     IGYFLWNEWNNLSLANVLFSVLLIGLVGMTLDLLFAQLQRKVTYVD"
     gene            263..1213
                     /locus_tag="VHV99_17470"
     CDS             263..1213
                     /locus_tag="VHV99_17470"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3637238.1"
                     /translation="MSTKSNAQTSGATSFVSVEGLTKRYRDDLPPVFENVSFTIEQGE
                     FICVIGHSGCGKSTILNVLAGLEQPSEGHVIVRNREISGPGLDRGVVFQNHALLPWMT
                     VEGNIAFAVRSRYPDWTREQIAVHGARFIEMVGLKGAEKKKPSELSGGMKQRVGIARA
                     FAIEPRMLLLDEPFGALDALTRGVIQDELLKICTATQQTVFMITHDVDEAILLSNKIM
                     LMSNGPRARIAEIVVNTLPANRQRETMHHDPQFYRIRNYLVDFLVRRSKELSASHGER
                     PDTGRVAAATTRLVYPGLDDGAATPAAVARPAGPNLKAIA"
     gene            1236..1685
                     /gene="cynS"
                     /locus_tag="VHV99_17475"
     CDS             1236..1685
                     /gene="cynS"
                     /locus_tag="VHV99_17475"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HEX3637239.1"
                     /translation="MISSRNEVTQMIVSAKVAKGLKWSEIAGKIGQSKEWTTAACLGQ
                     MTFSKQQAEIAGEIFGLPDEAVAWLQIVPYKGSLETAVPTDPLIYRWYEIVSVYGTTI
                     KELIHEEFGDGIMSAIDFSMDIQREADPKGDRVKVLLSGKFLPYKSY"
     gene            1773..>2600
                     /locus_tag="VHV99_17480"
     CDS             1773..>2600
                     /locus_tag="VHV99_17480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007738217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX3637240.1"
                     /translation="MLTTASPMQVEGFFDPGTWTVSYLLLDVRTGDCALIDSVLDYDP
                     KSGRTGTTTADRLVSRVHELGAKVQWLLETHVHADHLSAAPYLKERLGGKIAIGAQVT
                     RVQDVFGKLFNAGSLFAHDGSQFDHLLSDGECFAVGALQARAIHTPGHTPACMTYVVS
                     DGAQSVAFVGDTLFMPDYGTARCDFPGGDARTLYRSINKVLSLPADTKLYMCHDYQPG
                     GRPLQFVSTVAEEREHNVHIRDGIGEDAFVAMRTARDATLNMPVLMLPSVQVNMRAGH
                     "
BASE COUNT          503 a          795 c          808 g          494 t
ORIGIN      
        1 ccgccattgc gcagcgcgct gtgggtcgtg ctgcccgcgg cagctcccac catcgtcacc
       61 ggcatgcgga tttcgatggg catcgcgtgg ctcgtgatcg tcgcggccga aatgctggtt
      121 ggcggcaccg gtatcggcta cttcctgtgg aacgaatgga acaacctgtc actggccaac
      181 gtgttgttct cggtattgct catcggtctg gtcggcatga cactcgacct gctgttcgcc
      241 caacttcagc gcaaggtgac ctatgtcgac taagtcgaat gcccagacgt ccggcgctac
      301 gagtttcgtc agcgtcgaag gactgacgaa gcgctatcgc gacgacctgc ccccggtgtt
      361 cgaaaacgtc agcttcacga tcgagcaggg cgaattcatt tgcgtaatcg gccattccgg
      421 ctgtggcaag agcaccattt tgaacgtgct ggctggtctc gagcagccca gcgaggggca
      481 cgtgatcgtg cgcaatcgcg aaatttccgg accggggctc gatcgcggcg tggtgttcca
      541 gaaccatgcg ctgctgccgt ggatgaccgt ggaaggcaac atcgcgttcg cagtgcgctc
      601 acgctatcca gactggacac gcgaacagat cgccgtgcac ggcgcgcgct tcatcgagat
      661 ggtcggcctg aagggcgctg aaaagaagaa gccgtcggag ttgtcgggcg ggatgaagca
      721 gcgcgtgggc atcgcacggg cgtttgcgat cgagccgcgc atgctgttgc tcgacgagcc
      781 gttcggcgcc ctcgacgcgt tgacgcgcgg tgtgatccag gatgaattgc tgaaaatctg
      841 caccgcgacg cagcagaccg tattcatgat cacgcacgac gtcgacgaag cgattctgct
      901 gtcgaacaag atcatgctga tgagcaacgg gccgcgcgcc cggatcgccg agatcgtcgt
      961 caacacgctg ccggctaacc gtcagcgcga gaccatgcac catgacccgc agttttatcg
     1021 aatccgcaat tatctggtcg actttctggt gcggcgttcg aaagaactga gcgcctccca
     1081 cggcgagcga cccgataccg ggcgcgtggc ggccgccaca acgcggctcg tttatcccgg
     1141 gctcgatgac ggcgcagcca cgccggccgc agtcgcgcgg cctgccggac ccaatctcaa
     1201 ggcaattgcc tgatctctca cccaaggagc cacctatgat ttccagccgc aatgaagtaa
     1261 cgcagatgat cgtttccgcc aaggtcgcga agggcctgaa gtggagtgag attgccggca
     1321 agatcggcca gagcaaggag tggacgacgg ccgcgtgcct cggccagatg accttcagca
     1381 agcagcaggc cgagattgcc ggcgagattt tcgggctgcc ggacgaggcc gtcgcgtggt
     1441 tgcagatcgt cccgtacaag ggttcgctcg agaccgcggt gccgaccgat ccgttgatct
     1501 accgctggta cgaaatcgtc agcgtgtacg gcaccacgat caaggaactg attcacgaag
     1561 agttcggcga cggcatcatg agcgcgatcg atttttcgat ggatatccag cgtgaggccg
     1621 atccgaaagg cgaccgcgtc aaggtgctgc tgtctggcaa gttcctgccg tacaaatcgt
     1681 attaaccgcg ttcttcccgt ggcttcatcg ggacaagact gcagcgcggc gttactttga
     1741 gaaatcgatg gcccggaatg tttgaggaga tgatgttgac taccgcgagc cccatgcagg
     1801 tcgaaggctt cttcgacccc ggtacctgga cggtcagtta ccttctgctg gatgtccgca
     1861 cgggcgactg tgcactgatc gacagcgtcc ttgactacga cccgaagtcc ggtcgtaccg
     1921 gcacgacgac agccgaccgg ctcgtctctc gcgtccatga gcttggcgcg aaggtccaat
     1981 ggcttctcga aacccacgtg catgccgacc atctgtccgc cgcgccttac ctcaaggaaa
     2041 gactcggcgg aaagattgcg atcggcgcgc aggtgacgcg cgtgcaagac gtgttcggca
     2101 agctgttcaa cgcaggctcg ctattcgcac acgatggcag ccagttcgat cacctgctct
     2161 ccgacggcga gtgtttcgcc gtcggagcgt tgcaggcgcg cgccatccat actccgggcc
     2221 atacgccggc ctgcatgacc tatgtggtca gcgacggcgc gcagagcgtc gcctttgtcg
     2281 gcgacaccct gttcatgccc gattacggca ccgcgcgctg cgactttcct ggcggcgacg
     2341 cccgtacgct ttaccggtcg atcaacaagg tgttgagcct gccggcggac acgaagctgt
     2401 acatgtgcca cgactaccag ccgggcgggc ggcctttgca atttgtcagc acggtagccg
     2461 aggagcgcga acacaacgtc catatccgcg acggtatcgg cgaggacgcc ttcgttgcga
     2521 tgcgtaccgc gcgcgacgcg acactcaaca tgccggtgct gatgctgccc tcagtgcagg
     2581 tcaacatgcg cgcgggacat
//
LOCUS       DATASH010000648        16588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1096455,
            whole genome shotgun sequence.
ACCESSION   DATASH010000648 DATASH010000000
VERSION     DATASH010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 16588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16588
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1096455"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="VHV99_17485"
     CDS             complement(<1..861)
                     /locus_tag="VHV99_17485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer binding domain-containing protein"
                     /protein_id="HEX3637241.1"
                     /translation="MEHECDTLPHWLTGSPQGEASHVSAAYNAAVTAPPAYPAGTRQT
                     GKSAHAAEASRASTRTASGIAPAGFLTLFTEEEIGTPSPAARRATPVISPLLRFGSAQ
                     DRIEFVRQRIKQLGFDSFSYSATRTTAHHKTMFVLTSYESQTWLTRYFRERYFELDPR
                     VALASPTGMPFLWNTADMRADLPRAQMRSERLGGLIDMLEATGRKSGILTQMPLPEPE
                     LSASLCFNSEIGNPRWMTESIVAETLMFAHTIHEFIWTHAKSVIGIAPAQQQRVALSK
                     LQHAVLKAVVQ"
     gene            1060..1971
                     /locus_tag="VHV99_17490"
     CDS             1060..1971
                     /locus_tag="VHV99_17490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX3637242.1"
                     /translation="MARIPKLTTTLAYDRYMAGQKLVSSVDRPWRHLVLRSYLEPAEQ
                     ELLEAPGLQDVTLMTLGSGAEHLERNLNGRWESAELRVGDLWFVPPVPISWRWRSISD
                     EPLSTVHLHLERSLLDSVADQMELGGSRELSLGDAMQFHDPLIAAMLGALHRAAGDSA
                     DSRLYVDALVHGLAAHLLQHYSRGRHAEAGLPPQPERLVPRRIRRVTDYIRANLAADL
                     AIGELAALAGLSSFHFARVFRRETGETPHQFVTRLRLEEAARLLRATDQPVMQIAIAV
                     GFENASRFSVQFKRDYGVTPLAYRLRG"
     gene            2648..3073
                     /locus_tag="VHV99_17495"
     CDS             2648..3073
                     /locus_tag="VHV99_17495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3830 family protein"
                     /protein_id="HEX3637243.1"
                     /translation="MTRLRITSCGHVFTAETHPDAPQTVAAFLKLLPYRQKIIHVRWS
                     GEGCWVPLGDFKLENDGAAVGFENHTSHPSVGDILFYPGGYSETEIILAYGSCCFASK
                     LGQLAGNHFLTIVEGREKLRELGTKVLWDGAQEVLFEKI"
     gene            3250..4461
                     /locus_tag="VHV99_17500"
     CDS             3250..4461
                     /locus_tag="VHV99_17500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX3637244.1"
                     /translation="MTDSRVLPLAAFAAHLRQQQVDLTERWMKAVFNDVELTESDRLT
                     YEQLADHIPNILDEICSVLESQDLDELEPAIRRDARLHGKLRWKQGYQIDELVRELDL
                     FRQMLTGAIVEFSEARPSFTRRHEERARHFIDEAVSFVTLTSIREVVSERDRKIDDYT
                     AHIERVNHELTLKQRLVGDLYESRMQITRSVVHDLRNFLNVFSMALQLISRAPAKIDT
                     ALALANRQAADMKALVDELVEYSVVLGDTNPVTTERFALRELFDELVASCGPAIEAKG
                     LRLEAAFDGELDTVVSNRLKLKQVALNLLTNAAKYTKAGQVELAMSAAQHDRWCLRVS
                     DTGVGIRASDRERVFKEFERATADDIPGAGLGLAIVKELCRILGGEIRFDSQEGEGTI
                     FEITFPVRLDS"
     gene            4959..7586
                     /locus_tag="VHV99_17505"
     CDS             4959..7586
                     /locus_tag="VHV99_17505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HEX3637245.1"
                     /translation="MLNKTYKTVWNKTTRTYVAASEVAKRRGAKSASACGALVAAGAG
                     LLGGLAFALPVSAAGSIDQYIAVSTTLTDGSEPTSVDNSKLNAMALGPNAVSNGENAL
                     AVGAKTLASLDGTTAVGSAAAALAVNATVIGAGASTSSDALNGVAIGYLASAEGVNTL
                     GLGAHARAFGDDSIALGTGALTSGGAAKTIAIGSGASASAAGGIALGAESKADRTNAL
                     SVGNSSLSRQIINVAAGTQNSDVVNVSQLKGAMESIGGGATVNVDGTIRKPTYTISGQ
                     TYTDVGAALSALVANTAAASADSVQYDSTSHDTVTLGNIGTPVRVTNVSAGALSANSL
                     DAVNGSQLFATNEAVAQNTSDIAANTGSITNLDQRVTDNTTTISNLDARTTTIEGDVS
                     NITNQITNGEIGLVQQNQASRTLTVAKDTDGASVDFTGTGGARELTGIAAGTTDTSAV
                     NVGQFKPMVAALGGGAQINADGSVSGPTYHMQGGTQTTVGDALGSLDSGLTTLQQNME
                     TGGVGIVTQDSVSRIINVGATTDGALINMAGTAGNRVVTGVASGALNAASTDAINGSQ
                     LYTHAASTAVALGGGSTVNPDGSVTAPSYSVGGTVVNNVGSAITNLDGRVTQNSSDIA
                     GLQTTINNISGSVANAVQYDSSAHDKVTLGGTAANASRVTLTNLQDGELSATSTDAVT
                     GAQLWNTNQQLGNLGQQLVNINQVVQNNAVTGNAFVAVNSTGNAAQAIGNSSVAIGGG
                     AKASAPNSVAIGEGSVADLSNTVSVGSAGNERRITNVAAGQAPSDAVNMQQFQGGMSE
                     MARNAYSGTASAIALTMVPEVDAGKNLAIGVGTAGYKGYQAVAVGLSARVTQSLKVKL
                     GAGISSATTTVGAGAAYQW"
     gene            complement(7686..7814)
                     /locus_tag="VHV99_17510"
     CDS             complement(7686..7814)
                     /locus_tag="VHV99_17510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate:formate antiporter"
                     /protein_id="HEX3637246.1"
                     /translation="MSTMQAVHPTNKAKLAVFWLYVTLPLAWGVVNTLSQAMKLFK"
     gene            complement(7825..9228)
                     /locus_tag="VHV99_17515"
     CDS             complement(7825..9228)
                     /locus_tag="VHV99_17515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEX3637247.1"
                     /translation="MNSITEPNGNSGSAPFFSKQATVAQPGFSRWMVPPAALAVHLCI
                     GQAYAFSVFNGPLTKVIGITQSAADDWSLTSLGWIFSLAIVFLGLSAAFAGKWLEHVG
                     PRRTMFTAACCFGGGFLVSALGVYLHQIALLYLGYGVIGGIGLGLGYVSPVSTLIRWF
                     PDRRGMATGMAIMGFGGGAMIAAPLSVALMNHFRSATSIGVAETFIVLGIAYFISMSI
                     GALAIRVPPSDWKPAGWTPPATNQKKMISRNHVHIDQALKTPQFYLIWLVLFLNVTAG
                     IGILGQASVMIQESFKDTVTAAAAAGFVGLLSLFNMGGRFVWASASDWVGRKNTYFIF
                     FALGAVLYYVVPGFAASGQIALFVLAYCVILSMYGGGFATVPAYLADMFGTAFVGGIH
                     GRLLTAWAAAGVAGPVLVNYIRAYQVAHGVAKADAYTMTIHIMVVLLVIGFVCNLLVK
                     RVDDRHHMNDAQLAKGA"
     gene            9717..9938
                     /locus_tag="VHV99_17520"
     CDS             9717..9938
                     /locus_tag="VHV99_17520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637248.1"
                     /translation="MEFVPLSLFAVAVVGLGAAATVLLTHWIPQPVAQLAAQHGRFIG
                     LGEAGGMAAAAPYRARVQQEAMNVVRTSN"
     gene            9916..10635
                     /locus_tag="VHV99_17525"
     CDS             9916..10635
                     /locus_tag="VHV99_17525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX3637249.1"
                     /translation="MSSELQTEAVATPLTLSLQPIGASASLRDQAYAMLRQAIADADI
                     YQTREEVRLDERVLSESLGVSRTPVREAMTLLEQEGFLRMVPRRGIYIVRKSKREIVE
                     MIQMWAALESMAARLATLHATDAEIARLRHMFDHFHDATPAEHIAEYSDANIAFHQAI
                     VELSKSQIILDTIKNIFVHVRAIRRMTISQSDRASRSIVDHLRIIEALEQRDTELAER
                     LTREHSLDLAAFVEANCDFLD"
     gene            10890..12137
                     /gene="frc"
                     /locus_tag="VHV99_17530"
     CDS             10890..12137
                     /gene="frc"
                     /locus_tag="VHV99_17530"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HEX3637250.1"
                     /translation="MSKALDGVRILDFTHVQSGPTCTQLLAWFGADVIKVERAGAGDI
                     TREQLRDIPDVDSLYFTMLNHNKRSITIDTKNPEGKQVLETLIQQCDVLVENFAPGAL
                     DRMGFTWERIQELNPRMIVASVKGFGPGPYEDCKVYENVAQCAGGAASTTGFDDGPPV
                     VSGAQIGDSGTGLHLALGIVTALYQRTQTGRGQKVLAAMQDGVLNLCRVKLRDQQRLE
                     RTGTMKEYPQYPNGTFGEAVPRAGNASGGGQPGWILKCKGWETDPNAYIYFITQAPVW
                     AKICKVIGKEEWATHPDYATPAARLPHLKDIFAEIERWTMTKTKFEAMQILNKYDIPC
                     GPILSMKEIAEEPSLRKTGTIVEVDHPTRGKYLTVGNPIKLSDSPTEVKRSPLLGEHT
                     DEVMAELGYSPEQISALRTAGAI"
     gene            12270..13055
                     /locus_tag="VHV99_17535"
     CDS             12270..13055
                     /locus_tag="VHV99_17535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX3637251.1"
                     /translation="MDTWMRFMSSGGGVVFGRVEGGYLHEYESLDKPVPTGVVLSTRA
                     LTPLAPCAPGKVVALWNNYHALAAKLDKPVPSHPLFLLKPAGSVIGSGEPIRRPPSYA
                     GKIVYEGELGIVIGRRCRDASAEEAAESIFGYTLINDVTAADLLNENPHFPQWCRAKG
                     FDTFCCIGPSIVSGFDWRSARLVTMVDGVERQNYPLDDMVFSPAEQVRLISQDLTLEP
                     GDVIACGTSVGVGSIKDGATVTVSIDGIGTLSNTLAAVREIAL"
     gene            13052..13696
                     /locus_tag="VHV99_17540"
     CDS             13052..13696
                     /locus_tag="VHV99_17540"
                     /inference="COORDINATES: protein motif:HMM:NF014307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEX3637252.1"
                     /translation="MTRRPRLVALLGSAAGLAVVLGGFARYLSAASQANLERIELYAA
                     VFIGALVFATSAIAFCKMRGTLDLSRAAHPGHGIVNVSALLLCGWLGYGFVTEQAQPF
                     GLAALLAMSVLAAALGTHLMISREYSKDRGSRIHAFAGHCQGLTVERHGLLARVEWHG
                     GEEQAWALREITPKRVRAAAYRHRRGWHNTGNTSAQKRGSVRQRTSHRAMYRQG"
     gene            complement(13756..14058)
                     /locus_tag="VHV99_17545"
     CDS             complement(13756..14058)
                     /locus_tag="VHV99_17545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637253.1"
                     /translation="MNHASVESHASTHTSVSLDSPDDAHLSAFNAAFSDLGLRFRWDR
                     ATLDVLKEFTSEAARITAYIERFHAHLHRAYDAEFLAQMILSKKARYYREFAATGD"
     gene            complement(14167..15114)
                     /locus_tag="VHV99_17550"
     CDS             complement(14167..15114)
                     /locus_tag="VHV99_17550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3637254.1"
                     /translation="MTMRNATLRQLKVFETVARHLSFSRAAEELHLTQPAVSTQVRQL
                     EEHAGLPLFEQLGKKIYLTPAGTEMLHYSRAIIQQFHEVDEAMGQLKGVSGGKLNVAV
                     ISAGDYFFPRVLAEFTRRYSGVVLNLSVHNREELLHQLATNQTDLAVMVRPPHETDAT
                     NEPFAPHPYVIVAAPTHPLAHKRNIKMSQLASEAFIVRERGSDTWNSMEEGFAGRLSN
                     LKIAMEIKSTETIKQAVIAGMGIAFLSAHTISLELQLGHLVVLDVESFPVMLNWYVVH
                     RKNKRLPPVAVAFKRFLMEEGASLIEKITRVKELSVYKQ"
     gene            15492..>16588
                     /gene="oxlT"
                     /locus_tag="VHV99_17555"
     CDS             15492..>16588
                     /gene="oxlT"
                     /locus_tag="VHV99_17555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate/formate MFS antiporter"
                     /protein_id="HEX3637255.1"
                     /translation="MDDINQQTTARVFWANRWWQLVIGMMCMALVANLQYAWTLFVTP
                     MNARHHWGEASIQLAFAIFILTETWLVPVEGWLVDRFGPRPVVAGGAICAGLAWVMNS
                     YATTLPMLYVSAVIAGIGAGGVYGTCVGNALKWFPDRRGLAAGLTAAGFGAGAAVTVI
                     PIANMITRTGYEHTFFFFGILQGVSILFLALLLKKPNLRLQAAPKKKFAVTKVDYTPG
                     QMIRTPVFWVMYVSFVAVAAGGLMATAQIGPIAKDWGLARIPMTIFGMTLPLLTATLS
                     IDNICNGFTRPLCGFISDKLGRENTMFVIFIGEGLALLGLMQYGSNPYAFMTFAALIF
                     LFWGEIFSIFPAICADTFGSKYAAANAGTLYT"
BASE COUNT         3293 a         4790 c         5290 g         3215 t
ORIGIN      
        1 ttgcacgacc gccttcagca ccgcatgctg cagcttgctc agcgcgacgc gctgctgctg
       61 cgcgggcgcg atgccgatca cgcttttcgc gtgcgtccag atgaactcgt gaatcgtgtg
      121 tgcgaacatc aacgtctcgg ccacgatcga ttcggtcatc cagcgcgggt tgccgatctc
      181 cgagttgaag cacaggctcg cgctcagctc cggctcgggc agcggcatct gcgtgagaat
      241 cccgctcttg cggccggtgg cctcaagcat gtcgatcagg ccgccgagcc gctcgctgcg
      301 catctgcgcg cgtggcaggt cggcgcgcat gtcggcggtg ttccagagga acggcatgcc
      361 ggtcggcgag gccagtgcga cgcgcgggtc gagctcgaaa taacgctcgc ggaaatagcg
      421 cgtgagccat gtctgcgact cgtagctggt cagcacgaac atcgtcttgt gatgcgctgt
      481 ggtgcgcgtg gctgaatagc tgaacgagtc gaagccgagt tgtttgatgc gctgccgcac
      541 gaattcgatg cggtcttgcg cactgccaaa ccgcagcagc ggactgatca ccggcgtggc
      601 gcggcgcgcg gcgggcgagg gcgtgccgat ttcctcctcg gtaaacagcg tgaggaaacc
      661 tgcgggcgcg atgcctgatg cagtgcgggt ggatgccctg gatgcttcag ccgcgtgggc
      721 tgatttcccg gtttgccgcg tgccggccgg gtaggccggt ggtgcggtaa ccgcggcgtt
      781 gtaagccgcc gacacgtggc tggcttcgcc ttgcggcgag ccggtcagcc aatgcggcag
      841 cgtatcgcat tcgtgttcca tgaaacggta gctccggcgt tcgaaacgcg tgaaagacga
      901 caatctgaat gattagctat ttccaaaaat tacggtagcc ggttccttgc tatcattttt
      961 gagatcttgc tctgaccttg acctcagacc tcttaaccgg cgacgcgggt gcggcagggt
     1021 ttgctcatgt gcatgcggga cctcccagga agtgacgtga tggcaagaat tccaaagctg
     1081 acaacgacgc tcgcctacga ccgctatatg gcgggccaga agctggtctc gagcgtggat
     1141 cgcccgtggc ggcacctggt gctgcgctcg tacctcgaac cggccgagca ggaactgctg
     1201 gaggcgcccg gcctgcagga cgtcacgctg atgacgctcg gcagcggcgc tgaacacctc
     1261 gagcgcaacc tgaatgggcg ctgggagagc gccgaactgc gcgtgggcga cctctggttc
     1321 gtgccgcccg tgccgatctc gtggcgctgg cgttcgatca gcgatgagcc tctgtcgacc
     1381 gtccatttgc acctggagcg cagcctgctc gacagcgtcg ccgaccagat ggagctgggc
     1441 ggctcacgcg agctgtcgct tggcgatgcg atgcagtttc acgatccatt gatcgccgcg
     1501 atgctcggcg ccttgcatcg cgccgcgggc gattctgcag actcgcggct ctatgtcgac
     1561 gcgctggttc acgggctcgc cgctcatctg ttgcaacatt actcgcgcgg ccgtcacgcc
     1621 gaggccggcc tgccgccaca gccggagcgc ctggtgccac gccgcatccg ccgtgtaacc
     1681 gattacattc gcgccaatct tgccgccgat ctcgccatcg gcgaactggc tgcgctggcc
     1741 gggttaagca gttttcattt tgctcgggtg ttccgccgtg agaccggtga aacgcctcat
     1801 cagttcgtca cgcgcttgcg gctcgaggaa gccgcgaggc tgctgcgggc caccgatcag
     1861 cccgtgatgc aaatcgcgat tgcagttggg ttcgagaatg ccagtcgttt ctccgtgcag
     1921 ttcaagcggg actacggcgt tacgccgctt gcgtaccggc tgcgcggctg agagtcgggt
     1981 tgcgcggctg gatcgggtgg gcggtttgcc ggtctaaggg ctaccggcgt gtgggctgcc
     2041 cggcgtacgg ccttaccggc gtacaggcca ccggcttgcg cggcccaggc ggcatgccag
     2101 cagcgacagc tacacgcggg cacgtggacc cgcgtaagca ttcgccgaca tcgatgctgt
     2161 gcagcgcggg gatgcctggc ggccccgcgc tgcacaggaa tgtcaaggtc ggacgcggtg
     2221 ggttatcgag cgcaaagctc aaaatcgccg aaacggggat cacgcacgtt tcgcagtttg
     2281 ctgtgcattg cgcggcttgc aaactacgaa ctttcgtggc gcggttggtg atggacctgg
     2341 catgcacagg cctgatgctg tgccgacggt ttttcaggtc gtcgtgttca atatgtgatg
     2401 cagtatggat gcacggcaag cattgcgcct gctggggtgc ggaagttgac ggcagatcgc
     2461 cggggccggc ggcagcctgt gcgacgcgcg ctcaccattg cgactggtgc aaactggcct
     2521 gaccagatgc gcggcaattg tatctgtcga agcattgcac cggccgacag aatgtttcgc
     2581 ttccggcttg gccccagccg gcgttcgacc gaccattcac tcaacccgtc acaccggata
     2641 tctagcgatg acccgacttc gaatcacctc ctgcggccac gtctttaccg ccgaaacgca
     2701 tcccgacgcg ccgcaaacgg tggccgcctt cctgaagctg ttgccgtacc gtcagaagat
     2761 catccacgta cggtggagcg gcgagggctg ctgggtgccg cttggcgact tcaagctgga
     2821 aaacgacggc gccgcggtcg ggttcgaaaa tcatacgagc cacccgtcgg ttggcgacat
     2881 cctgttctat ccgggcggtt acagcgaaac cgagatcatt ctcgcctacg gttcgtgctg
     2941 cttcgcaagc aagctgggcc agctggccgg caatcacttt ctgaccatcg tcgaaggccg
     3001 cgaaaagctg cgcgaactcg gcaccaaggt gctgtgggac ggcgcacaag aggtgctgtt
     3061 cgagaagatc tgaagcggct caaaccctgg ttaatttttg ttgctcaaat cgagcatact
     3121 ggaggcgccg tggcggcttg cccaagtccc cacgatctgc ctcctgaacg tgattcgcgg
     3181 tccgatccgg tcgcgttcgg tagggcacga aacttttact tcgccgccag gcgacgccag
     3241 gttccctaca tgacggattc gcgcgtactt ccgttggctg ctttcgccgc gcatttgcgc
     3301 cagcaacagg tcgatttgac cgagcgctgg atgaaggcgg tgttcaatga cgtcgagttg
     3361 accgaatccg accggctgac ctacgaacaa ctcgccgatc acataccgaa catcctcgac
     3421 gaaatctgca gcgtgctgga aagccaggat ctcgacgaac tggaacccgc gatcaggcgc
     3481 gacgcgcgcc tgcacggaaa acttcgctgg aagcagggtt accagatcga cgaactggtg
     3541 cgcgagctcg atctgttccg gcagatgctg accggcgcga tcgtcgagtt cagcgaggcg
     3601 cggccgtcgt tcacgcggcg tcatgaggag cgggcccggc atttcatcga tgaagccgtg
     3661 agcttcgtca cgctgacgtc gatccgcgag gtcgtcagcg agcgtgacag gaagatcgac
     3721 gattacaccg cgcacatcga gcgcgtcaat cacgagctga cgctcaagca acggctggtc
     3781 ggcgatctgt acgaatcgcg tatgcagatc acgcgcagtg tcgtgcacga tctgcgcaac
     3841 ttcctcaatg tgttttcgat ggcgctgcaa ctgatcagcc gcgcgccggc gaagatcgac
     3901 accgcgctcg cactggcgaa ccgccaggcg gccgacatga aagcgctggt cgacgaactg
     3961 gtcgaatact cggtggtgct cggggatacc aaccccgtga cgactgagcg ctttgcgttg
     4021 cgcgaactat tcgatgaact ggtggcctcg tgcggaccgg cgatcgaagc gaagggcttg
     4081 cgcctggagg ccgccttcga cggcgagctg gacacagtgg tctccaatcg gctgaagctc
     4141 aagcaggtcg cgctcaacct gctgactaac gccgcgaaat ataccaaggc gggccaggtt
     4201 gagctggcca tgtccgccgc gcagcacgac cgctggtgtt tgcgggtgtc ggatacgggc
     4261 gtggggattc gcgcgtcgga tcgtgagcgt gtgttcaagg aattcgagcg cgccaccgcg
     4321 gacgacattc ccggcgccgg tctcggtctg gccatcgtca aggaactgtg ccggatcctg
     4381 ggcggagaga tccgtttcga ctcgcaggaa ggcgagggga cgatcttcga gattacgttt
     4441 ccggtgcgcc tcgacagtta gtcatgtcta tcgcggcgag cggcatcaga ccgctcgctg
     4501 atccttcccg ttcgatgcgg tgttacgtcg ctagctcaat gcccgtcctc ctggtttaag
     4561 aaacattaag agtgcttctt aacactacct aaaaatatcc cggttgattt aagacgtttg
     4621 gcgctttgcg tcctaaacgg ggtatgtcgt ttgtactcat tcaagctaaa aaatcgcttt
     4681 taggagtttt cgtatttcac catcatgtaa gcgaacggca aactatggag gtcgcgtttg
     4741 tcgatcaggt gaaggcgcga tgcatcctta agtttgtcgg cgtgtgagtt acatatgtat
     4801 ggcagccgtc tcgtcggttg gcggcagact agcggcatcg cgtttgtcgg ttggccgaaa
     4861 gcgcgctgca ttcttaagtt tctctgcgtt tgagctagat atttaggatg tcgtaagaaa
     4921 aatcttgatg aattggttcg ataaaaagat tgggaggaat gttgaataag acttacaaga
     4981 ccgtttggaa taaaacgacc cgcacgtacg tcgccgcatc ggaggtggcg aagcgccgtg
     5041 gcgcgaagag cgcgtcggca tgtggagcgc tggtggcagc tggggccggg ttgttgggtg
     5101 gattggcgtt tgcgctgccg gtgagtgcgg cggggtccat tgatcaatac atcgctgtta
     5161 gcacgacatt gacggatggc agcgagccta cctcggtgga caattcgaag ttgaatgcaa
     5221 tggccttggg cccgaatgct gtctcgaatg gcgaaaacgc gttggcggtc ggtgccaaga
     5281 ctctggcgag cctcgacggc acgacggcag tcggttcggc agcggctgca ttggctgtga
     5341 atgccacggt gatcggtgca ggcgcttcga ccagcagcga cgcgttgaat ggcgtggcaa
     5401 tcggctatct ggcctctgcg gaaggtgtca acacgctggg ccttggggcg catgcacgtg
     5461 cgttcggcga cgattcgatt gcgctgggca ccggcgcctt gacatccggc ggcgctgcta
     5521 aaactatcgc catcggctct ggcgcatccg ccagcgccgc cggcgggatc gcgctcggcg
     5581 ccgagtcaaa agctgatcgg accaatgcac tctcggttgg gaattcgagc ctcagtcggc
     5641 aaatcatcaa tgtggccgcc ggcacccaga acagcgatgt ggttaatgtt tctcaactga
     5701 agggcgcgat ggagtccatc ggcggaggcg cgaccgttaa tgtggatggc acgatcagga
     5761 agccgacgta cacaatcagc ggtcagactt acaccgatgt cggcgcggct ttatccgctt
     5821 tagtggcaaa cacggcagcg gcgagcgccg actcggttca gtacgactcg acctcgcatg
     5881 acacggtgac gttaggcaac atcggcactc cggttcgagt gaccaacgtg agcgccggag
     5941 cactctccgc caacagtctg gacgccgtga acggttcaca actgttcgcg accaacgagg
     6001 ccgtggcgca gaataccagc gacattgccg ctaacaccgg cagcatcacc aacctcgatc
     6061 agcgcgttac cgacaacacc accactatca gcaacctcga cgcgcgcacc acgacgatcg
     6121 aaggggacgt gagcaacatt accaatcaga tcacgaacgg cgagatcggg cttgtccagc
     6181 aaaatcaggc gtcgcgcacg ctcacggtgg ccaaggatac cgacggtgcc agcgtcgatt
     6241 tcaccggcac gggcggggcg cgcgagctga cgggtatcgc ggcgggcacc acggatacgt
     6301 ccgcggtcaa tgtcggccag ttcaagccca tggtggccgc gctcggcggc ggcgcgcaga
     6361 tcaatgccga cggttcggtc agcggtccca cgtatcacat gcaaggcggt acgcaaacta
     6421 cagttggcga tgcgctcggc tcgctcgaca gcgggctcac caccttgcag caaaacatgg
     6481 agaccggcgg cgtcggcatc gtcacgcaag attccgtgtc ccgcatcatc aacgtcggcg
     6541 cgacgacgga cggcgctctc atcaacatgg ccggcacggc gggcaatcgg gtggtcaccg
     6601 gcgtggcgtc cggcgcgctc aacgccgcca gcaccgatgc gatcaacggt tcgcaactct
     6661 atacgcacgc agcgagcacg gcggtggcgc tcggcggcgg ctcgaccgtc aatccggacg
     6721 gctcggtgac ggcgccgtcg tacagcgtgg gtggcacggt cgtgaacaac gtcggcagtg
     6781 cgatcaccaa tctggatggc cgcgtgacgc agaacagcag tgatattgcc ggactgcaaa
     6841 ccacaatcaa caacatcagc ggttctgtgg cgaatgcggt tcagtacgac agttccgcgc
     6901 acgacaaggt cacactcggc ggcacggcgg ccaacgcgtc gagggtcacg ctcaccaacc
     6961 tgcaggacgg tgagctatcg gctaccagca ccgatgccgt caccggcgca caactctgga
     7021 acaccaacca gcagctcggc aacctcggtc agcagcttgt caatatcaat caggtggtcc
     7081 agaacaacgc agtcaccggc aacgccttcg tcgccgtcaa cagcacaggc aacgcggcgc
     7141 aggcaatcgg caacagttcg gtggcgatcg gcggcggcgc caaagcgagt gcgccgaact
     7201 cggtcgcgat cggcgagggc tcggttgccg atctgtcgaa cacggtatcg gtcggttcgg
     7261 cgggcaacga acgccgcatc accaacgtcg cggcgggaca ggcgcccagc gacgccgtca
     7321 acatgcagca gttccagggc ggcatgagcg agatggcgcg caacgcgtat tcggggacgg
     7381 cgtcggctat cgcattgacc atggttccag aggtcgatgc gggcaagaac ctcgcgatcg
     7441 gtgtcgggac ggccggctat aagggctatc aggcggtggc ggtgggcttg tcggcccggg
     7501 tcacgcagag cctcaaggtg aagctcggtg cgggcatcag ttctgccacg acgacagtcg
     7561 gcgccggtgc ggcgtaccag tggtagccgg gaaagaaggg tgagagaacg cgcgcggttt
     7621 gcgcgcgcgt cgtcaagctt ttctcacacc ccgcacgttc ggtcgtgcgg ggtgcggtgc
     7681 aacgcttact tgaacagctt catcgcttgc gagagcgtat tgaccacgcc ccacgccagc
     7741 ggcagcgtca catacagcca gaagacggcg agtttggctt tgttggtcgg atgaacggct
     7801 tgcatcgtcg acatactggt ctccttaggc gcctttggcg agttgtgcgt cgttcatgtg
     7861 gtgcctgtcg tcgacacgct tgacgagcag gttgcagaca aagccgatca ccagcagcac
     7921 caccatgatg tggatcgtca tcgtgtaggc gtctgccttg gcaacgccgt gcgccacttg
     7981 ataggcacgg atgtagttca ccagcacggg acccgcgaca cccgctgcgg cccaggccgt
     8041 cagcaagcgg ccgtgaatgc cgccgacgaa cgcagtgccg aacatgtccg cgagataggc
     8101 gggcactgtc gcgaagccgc cgccgtacat ggacaggatc acgcagtacg cgagcacgaa
     8161 cagggcgatc tggccggatg cggcgaagcc gggcacgacg tagtacagca cggcgccgag
     8221 cgcgaagaag atgaagtagg tgttcttgcg gccgacccag tccgaagccg aggcccacac
     8281 gaaacggcca cccatgttga acagcgacag cagaccgacg aaacctgccg ccgcggcagc
     8341 tgttacggtg tccttgaagc tctcctgaat catcaccgaa gcctggccga gaatgccgat
     8401 gcccgccgtc acattcagaa acagcaccag ccagatcaga tagaactgcg gtgtcttcaa
     8461 cgcctgatcg atatgcacgt ggttgcgcga aatcatcttc ttctggttgg tcgcgggcgg
     8521 cgtccagccg gccggcttcc agtctgaggg cggcacgcgg atcgcgagtg cgccgatcga
     8581 catcgaaatg aagtaggcaa tgccgagcac gatgaaggtc tcggccacgc cgatgctggt
     8641 cgcgctgcgg aagtggttca tcagcgccac cgacaaaggc gcggcgatca tcgcgccgcc
     8701 gccgaagccc atgattgcca tgccggtggc catgccgcgg cggtccggga accagcggat
     8761 caaggtcgac accggcgaca catagcccag gccgagtccg atcccgccga tgacgccgta
     8821 gccgagatag agcagcgcga tctgatgcag gtacacgccg agcgcggaga ccaggaagcc
     8881 gccgccgaag cagcatgccg cggtgaacat ggtacggcgc gggccgacat gttcgagcca
     8941 cttgccggca aacgccgccg acaaaccgag gaacacgatc gccagcgaaa agatccagcc
     9001 gagtgaggtc aacgaccagt cgtcagcggc ggattgcgta atgccgatga ctttggtcag
     9061 cggaccgttg aacacggaga aggcgtacgc ctggccgata cagagatgca cggcgagggc
     9121 tgcgggcggg accatccagc gcgaaaaacc cggctgcgca acggtggcct gtttggaaaa
     9181 gaagggggca gagcctgaat tcccgttcgg ctcggtgatg ctattcatgg tcggtctccg
     9241 tgacgaatgc ttttgttatc ggcgctttac gtacatttcc ttaatgccgc agcgtaaaga
     9301 acgtcgctcc gcgcgccgcc aatatatgca ttatgatttc atatatcgtg tgcgagttca
     9361 taaaaaactc gctgcgaaga acgatagggt gcgagcgtgg gcttcgcaat atgagatgta
     9421 aatacataag gtattagcct ggtgaaatgt ggccgtaatt tccggatcgc ggcaatgcag
     9481 tgtgacgatc aggaatgaat taccgggttc ggacgcaaaa agcggccgga ctcggtatga
     9541 gggtcgggcc ggacgatgag gaatgcgaag gcacgcggat aacagagaat gcatgctacc
     9601 ttcagtagac aaccctaaaa caacatcgac tgatatacta catacggaat attgatttct
     9661 gcggcattgc agcatatgat tggggtcttc cctaactcga tcattcggag ctacaaatgg
     9721 aatttgttcc tctctcgctg tttgccgttg ctgtcgtggg tttaggcgct gccgccaccg
     9781 ttctgttgac ccactggatt ccgcagccgg ttgcgcagct tgccgcgcaa catggccggt
     9841 tcattggctt aggcgaagcc ggcggcatgg ctgcggcggc gccataccgt gcgcgcgttc
     9901 aacaagaggc gatgaatgtc gtcagaactt caaactgaag cagtggccac acctttgacg
     9961 ttgtcgttgc agcccatcgg cgcgagcgcg agcttgcgcg accaggcgta tgcaatgctc
    10021 cgccaggcta ttgccgacgc ggatatttac cagacgcgcg aggaagtccg tctcgacgaa
    10081 cgcgtgctga gcgaatcgct gggcgtgagc cgcacgccgg tgcgcgaggc aatgacgctg
    10141 ctcgaacagg aaggtttttt gcgcatggtg ccgcggcgcg gcatctatat cgtgcgtaag
    10201 agcaagcgcg agatcgtcga aatgatccag atgtgggccg cgctcgaaag catggcggcg
    10261 cgtcttgcca cgctacatgc cacggacgcg gaaatcgccc gcctgcgcca catgttcgac
    10321 catttccacg acgccacgcc ggccgaacat atcgccgagt attcggacgc caacatcgcg
    10381 tttcatcagg cgatcgtcga gttgtcgaaa tcgcagatca ttctcgacac gatcaagaac
    10441 atctttgtcc atgtgcgggc aattcgccgc atgaccattt cgcagagcga ccgcgcgtcg
    10501 cgctcgatcg tcgaccacct acgcatcatc gaggcgttgg agcagcgcga taccgagctt
    10561 gccgagcggc ttacacgcga gcattcgctc gacctggcgg cgtttgtcga agcgaattgc
    10621 gattttctgg attgacggcg acttgagcgc cgggttggcg ctacggattg gaaaagcctg
    10681 cagtgacgtc gtcgctgcag gcttttttta tgggttaaat ccaatgtggg ggcggtggca
    10741 gggttaagcc ccgcttgacg atccatgaaa tatggtatgt gatatatcaa acgagttcag
    10801 gaaccaccta tccgcgcgag cgggcgttat cgaagcgggc gcgtcggtcg atgacgttgc
    10861 acacaaacga ttgatcagga gacttggaaa tgagcaaagc actcgacggc gtgcgcattc
    10921 tcgatttcac gcatgtgcaa tcgggcccga cctgcacgca attgctcgcg tggtttggtg
    10981 cggacgtgat caaggtggag cgggccggtg ccggcgacat cacgcgtgag caactgcgcg
    11041 acatccccga cgtggacagt ttgtacttca cgatgctcaa tcacaacaaa cgctcgatca
    11101 cgatcgatac gaagaatccg gaaggcaagc aggttctcga gacgctgatc cagcagtgcg
    11161 acgtactggt ggagaatttc gcgcccggtg cgctggaccg gatgggtttc acatgggagc
    11221 ggattcagga attgaatccg cgcatgatcg tcgcttcggt gaagggtttc ggccctggtc
    11281 cctacgagga ctgcaaggtc tacgagaacg tcgcgcaatg cgcgggcggc gcggcttcga
    11341 ccaccggttt cgacgatggg ccgccggtag tgagcggcgc gcagatcggc gacagcggca
    11401 cgggcttgca tctggcgctc gggattgtca cggcgcttta tcagcgcacg caaaccggcc
    11461 gtggccagaa ggtgctcgcc gcgatgcagg acggcgtgct caacctgtgc cgcgtgaagc
    11521 tgcgagacca gcagcggctc gaacgcaccg gcacgatgaa agagtatccg caatatccga
    11581 acgggacgtt cggcgaagcg gtgccgcgtg cgggcaatgc ttcgggcggc ggccagccag
    11641 ggtggattct gaagtgcaag ggctgggaga ccgatccgaa cgcctatatc tacttcatca
    11701 cgcaggcgcc ggtgtgggcg aagatctgca aggtgatcgg caaggaagag tgggccaccc
    11761 atccggacta cgcgacgcct gccgcgcgtt tgccgcatct gaaggacatc ttcgccgaga
    11821 tcgagcgctg gaccatgacc aagaccaagt tcgaggccat gcagatcctc aataaatacg
    11881 acatcccttg cgggccgatc ctgtcgatga aggaaatcgc cgaagagccg tcgctgcgca
    11941 aaaccggcac gattgtcgaa gtggatcatc cgacccgcgg caagtatctg acggttggta
    12001 atcctatcaa actgtcggac agcccaaccg aagtcaaacg ctcgccgctg ctgggtgagc
    12061 acaccgacga ggtgatggcg gaactcggct attcgcccga gcagatcagc gccttgcgca
    12121 ctgccggcgc gatttgagcg cgtagacgtg tcggcacgcg cctggcgcgg ggctgatcca
    12181 atgcattgat gataaaggcg cgatcttcct cgcagcacgc gatctggaag agcggggagt
    12241 atcgcgccgg ttcgctcaag gagtgacgaa tggatacgtg gatgcgtttc atgtcgagcg
    12301 gcggtggcgt cgtgttcggc cgggtggaag gcggctacct gcacgagtac gaaagtctcg
    12361 ataaaccggt gccgaccggc gttgtgttgt cgacacgtgc actcacgccg ctcgcgcctt
    12421 gtgcgcccgg caaggtcgtc gcgctatgga ataactatca cgcgctcgcc gcgaagctcg
    12481 acaagcctgt gccgtcacac ccgctgtttc tgctgaagcc ggcgggatcg gtgatcggct
    12541 cgggtgaacc gatccggcgg ccgcccagtt acgcgggcaa gatcgtctac gaaggcgagc
    12601 tcggtattgt gatcgggcgg cgctgccgcg atgcgagtgc tgaagaggcc gctgaatcga
    12661 tcttcggcta tacgctcatc aacgacgtga ccgccgccga tctgctgaac gagaatccgc
    12721 acttcccgca gtggtgccgg gcaaaaggtt tcgatacgtt ctgctgtatc ggcccctcga
    12781 tcgtgtccgg tttcgactgg cgctcagcgc gactcgtcac gatggtcgac ggtgtggagc
    12841 ggcagaacta tccgctcgac gacatggtgt tttcgccggc cgagcaggtg cgtctgattt
    12901 cgcaggacct gacgctcgag ccgggcgatg tgatcgcgtg cggcacgtcg gtgggggtcg
    12961 gctcgatcaa ggacggcgcg acggtgacgg tctcgatcga cggaatcggc acattgagca
    13021 atacgttggc tgctgtgcgc gagatcgcgc tatgacgcga cggccgaggc tcgttgcgtt
    13081 gctcggcagc gccgcagggc tcgcagtcgt gttgggcgga tttgcgcgct atctgtcggc
    13141 ggcgtcgcag gcgaatctcg aacgtatcga gctttatgcc gcggtcttca tcggcgcgct
    13201 ggtcttcgcc acgtccgcaa tcgcattctg caagatgcgc ggtacgctgg accttagcag
    13261 ggcggcgcat ccgggccatg gcattgtcaa tgtgtcagcg ctgctgctgt gtggatggct
    13321 cggctatggc ttcgtcactg aacaggcgca gccgttcgga cttgcggcgc tgcttgcaat
    13381 gagtgtgctg gctgccgcgc tgggcacgca tctgatgatc agccgcgaat actcgaagga
    13441 tcgcggttcg cgcattcatg cgttcgccgg acattgccag ggattaacgg tcgagcggca
    13501 tggtttattg gcgcgcgtcg aatggcacgg tggcgaagag caagcttggg cactgcgcga
    13561 gataacgccg aaaagagtgc gcgcggcagc ataccggcat cgccgcggct ggcataacac
    13621 tgggaacacg agcgcgcaaa agcgcggctc tgtccgccaa cgcaccagtc accgtgcgat
    13681 gtaccgtcaa ggatgaatac accaacgcgt cgagcggcaa acggaacgca tggcggggtc
    13741 gtcccgccga cacgctcaat cgccggtcgc cgcgaattcg cgatagtacc gggccttctt
    13801 gctcaggatc atctgggcaa gaaactcggc gtcgtatgcc ctgtgaagat gggcgtgaaa
    13861 ccgttcgatg taggcggtga tgcgcgccgc ttcactggtg aattccttca acacgtcgag
    13921 tgtggcccgg tcccagcgga accgcaggcc aagatcgctg aaggcggcgt tgaacgcact
    13981 cagatgggcg tcatcgggac tatccaggct cacgcttgta tgcgtgctgg catgagactc
    14041 aaccgaagcg tggttcatga tcggcctcct gggctgacaa gtggaagaac tcaagcgaga
    14101 gctcacttgc agcataggga tgccgatcaa tttgcaataa ttaaagtttt attggctttg
    14161 catagcctac tgcttataga cgctcaattc cttcacgcgc gtgattttct cgatgagact
    14221 cgcgccttcc tccatcagga aacgtttgaa ggcgacggcg accggcggca ggcgcttgtt
    14281 cttccgatgc acgacgtacc agttgagcat gacgggaaag ctttcgacgt cgagcacgac
    14341 cagatggcca agctgcaact cgagactgat cgtatgcgcg gacaggaagg caatgcccat
    14401 gccggcgatg accgcctgct tgatcgtctc ggtgctcttg atctccattg cgatcttgag
    14461 attggagaga cggccggcga agccttcttc catggaattc cacgtgtccg agccgcgctc
    14521 gcgcacgatg aaagcctcgc tcgccaactg gctcatcttg atgttgcgtt tgtgcgcgag
    14581 cggatgcgtg ggcgccgcca cgatcacata agggtgcggc gcgaacggct cgttggtggc
    14641 gtcggtttcg tgcggcgggc gcaccatcac cgcgagatcg gtctgattgg ttgcgagctg
    14701 atgcaacagc tcctcgcgat tgtgcacgga gagattgagc acgacgcccg agtagcgccg
    14761 cgtgaactca gccagcacgc gcggaaagaa gtagtcgccg gcgctgatca ccgcgacgtt
    14821 cagcttgccg ccggagacgc ccttgagttg acccatcgcc tcgtcgactt cgtggaactg
    14881 ctgaatgatc gcgcgactgt agtgcagcat ctcggtgccg gccggcgtca gatagatttt
    14941 tttgccgagc tgctcgaaca gcggcagccc tgcgtgttcc tcgagttgcc ggacctgggt
    15001 ggagacggcg ggctgcgtca gatgcagttc ttccgcggca cgcgagaagc tcaggtggcg
    15061 cgctaccgtt tcaaagacct ttagttgccg cagagttgca ttgcgcatcg tcatgaccga
    15121 tgtataaaca aacgtgaatc aacataataa caaactttaa ttattagtaa ttcagcaatg
    15181 accctagcat gggcctgtat aagaagcaga attgtcggtg caacactcgg gttaacgctt
    15241 ggatttgagg cggggcgggg gtagcagaga aaagttgata cactgccatt gcctaattga
    15301 tcggaataaa gaactgactt gtaaaagcag tcactcaggc aaaacggctt ttaaataatt
    15361 agaattgaag gaaggcgcaa gtattaaata ttcccggatg gcaaggctgt tcgggtcata
    15421 acgataagac gagacgcaca cagtacggcg cgacagccgg ccgtactgaa taaatagcgg
    15481 agacaaggcg catggacgat atcaatcagc agaccacggc tcgcgtattc tgggcgaacc
    15541 gttggtggca gctcgtcatc ggcatgatgt gcatggcatt ggtggccaat ctgcaatacg
    15601 catggacgtt gttcgtcacg ccgatgaatg cgcggcatca ctggggcgaa gcatccattc
    15661 aactcgcctt cgcgattttc atcctcactg aaacgtggct cgtgccggtg gagggctggc
    15721 tggtcgacag gttcggaccg cgtccggtgg tcgccggcgg cgcgatatgc gccggtctcg
    15781 cgtgggtcat gaattcgtat gcgacgacct tgccgatgct gtatgtgtca gcggtcattg
    15841 ccgggatcgg cgcgggcggc gtgtacggca cctgtgtggg caatgcgttg aagtggtttc
    15901 cggatcgccg cggtcttgcg gccggtctga ccgcagcggg ttttggcgcc ggcgcggccg
    15961 tgaccgtcat tccgattgcc aacatgatta cgcgcacggg ctacgagcac acgttcttct
    16021 tcttcggcat tctgcaaggc gtatccatac tttttctcgc gttgctgttg aagaagccga
    16081 acctgcgtct gcaggccgca ccaaagaaga agttcgcggt gaccaaggtc gattacacgc
    16141 caggccagat gatcaggacg ccggtgttct gggtgatgta tgtgtcgttc gtcgcggtgg
    16201 ctgccggcgg cttgatggcg accgcgcaaa ttggcccgat cgccaaagac tgggggctcg
    16261 cacgcatccc catgacgatc ttcggcatga ccttgccgct actcaccgcc acgctttcca
    16321 tcgacaacat ctgcaacggt tttacgcgtc cgctgtgcgg tttcatctcc gacaaactcg
    16381 gccgcgaaaa cacgatgttc gtgatcttca tcggcgaagg cctcgccttg ctcggcctca
    16441 tgcagtacgg cagcaacccg tatgcgttca tgacgttcgc cgcgttgatc ttcctgttct
    16501 ggggtgaaat tttctcgatc ttccccgcca tttgcgccga caccttcggc agcaaatatg
    16561 cggcggccaa tgcgggtacg ctttatac
//
LOCUS       DATASH010000649         3621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6434342,
            whole genome shotgun sequence.
ACCESSION   DATASH010000649 DATASH010000000
VERSION     DATASH010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6434342"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..659)
                     /locus_tag="VHV99_17560"
                     /pseudo
     CDS             complement(1..659)
                     /locus_tag="VHV99_17560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588915.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
     gene            complement(888..2594)
                     /gene="recJ"
                     /locus_tag="VHV99_17565"
     CDS             complement(888..2594)
                     /gene="recJ"
                     /locus_tag="VHV99_17565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEX3637256.1"
                     /translation="MTRIVTRAASPVDADILTRHGLHPVLARLYAARGVCMPDEIETG
                     LARLVPPAGLKGCENAAALLADAIQGKRRMLVVADYDCDGATACAVAVRGLRMFGGQI
                     EYLVPNRFEYGYGLTPEIVALAARSASGKPELLITVDNGIASVDGVEAANALGIDVLV
                     TDHHLPGDELPAARAIVNPNQPGCTFPSKCIAGVGVMFYVLLALRAELRRRGAFNDAF
                     PEPRLDGLLDLVALGTVADVVKLDGNNRVLVAQGLQRIRKGKMQPGIAALFRAAARDA
                     RSASGFDLGFALGPRLNAAGRLSDMSLGIECLTTDDIGRAWDLAQQLDTMNRERREIE
                     AGMQQQALEDLSAINPDGATTITLFNPGWHQGVIGIVAGRLKEKFHRPSFTFALADDS
                     GQLVKGSGRSISGFHLRDALDLISKREPGLIVKFGGHAMAAGLTMAAADVARFTAAFE
                     AVGREWLSEEALSRTVETDGELEDAYFTPQFVEMLDAAVWGQGFPAPVFSGEFDIASQ
                     ALVKDKHLKLQLVRGRQRFNAIWFNHTDTLPARTTVAYRLSSDTWNGVSRVQLIVEHA
                     AS"
     gene            complement(2623..>3621)
                     /locus_tag="VHV99_17570"
     CDS             complement(2623..>3621)
                     /locus_tag="VHV99_17570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HEX3637257.1"
                     /translation="APLAPYMLRADGGDPGHATWACVQPVHVRIAHDHLVLIDPASLE
                     LSDDEASVLLAVARPLIEDLGVRIEAPKPARWYLSGDGFGTLAGASPLRASGRNIEIW
                     LPHEAHSGERSRAWMKLQNEVQMAWFEHPVNEAREARGLPAVNSIWFHAQGAVQPVTS
                     PFAQVYSDAAATRGLALSAGVAAAAPPASFAGLRAASVAGNGSRSASGDALGDAPGAA
                     SGGASGRAPGAAPAGASAVNASANANSHVDGVTLVELDPFSAPYIEQDWARWNDAFAA
                     LQTDWFKPALAALQSGQLAELGLTLCGDTGSVTLTVTRGDLRKFWRRRVLASLFIE"
BASE COUNT          594 a         1170 c         1228 g          629 t
ORIGIN      
        1 agaacgacgt atggcggccg ccggacgaat cgaacggcga cttgcgcacg aggcggtgca
       61 cgccggtttc ggtgcgcaaa aagccgtatg cgtattcgcc ttcgatcttg atcgtggcgt
      121 tcttgatgcc cgcgacgtcg ccttcggtct gttccagcac ttcggtcttg aagcccttgc
      181 gctcgcagta acgcaggtac tggcgcagca gcatggcggc ccagtcgcac gcttcggtgc
      241 cgccggcgcc cgcttgaatg tcgaggaagg cgttgttcgg atcggccgga ttcgcgaaca
      301 tccggcggaa ttccatgtcg gcgacgcgtt gttcgatgcc ttgcgcgtct gactcgcagg
      361 cgaggagggt ttcctcgtcg tcttcttcgc gggccatgtc gaacaggtct tgcgcatcgc
      421 gcaggtcgtt atcgagcgag gtgagtttgc cgacggtatc gtcgagcagt ttcttttccc
      481 ggccgagcgc ctgggcgtgc ttcgagtcgt tccagacgtc cgggtcttcg agttcccggt
      541 tgacttcgat tagacgtaat gccttgtcgt cgtagtcaaa gatacccccg tagcgcgccc
      601 gcgcgcgtgc gcaggtccgc cagagaggct tcgatcgcgt tgagacgttc cgcttccatg
      661 tcgatctttt atagcttcgt aaaaagggga aattatagcc gatcggcgcc ccgccccggc
      721 ggcttgtgag cggcgcggcg cgggtttggg gttgattttg cggtgatttt cgatgctttc
      781 gcgcagcgcc aggcaagctg cggtgcactc ggccatcgct agccggcgca cgcgttacgc
      841 gcggacttca cggcacggac gccggggggc gacagtctgg gcgcgcttca gctcgcggcg
      901 tgctcgacga tcaattgcac tcgagacacc ccgttccacg tgtcgctcga caagcggtaa
      961 gcgaccgtag tgcgcgctgg cagcgtgtca gtgtgattga accagatcgc gttgaagcgc
     1021 tggcggccgc gcacgagttg cagtttcagg tgtttgtcct tcaccagcgc ctgcgacgcg
     1081 atgtcgaact cgccggaaaa taccggcgcc gggaagccct gaccccatac ggcggcgtcg
     1141 agcatttcga cgaattgcgg cgtgaagtag gcatcttcca gttcgccgtc ggtttcgacc
     1201 gtacgcgaaa gcgcctcttc ggagagccat tcacggccca ctgcctcgaa cgcggcggta
     1261 aagcgggcca cgtcggcggc ggccatcgtc aggcccgccg ccatcgcgtg gccgccgaat
     1321 ttgacgatca ggcccggttc gcgcttcgag atcaggtcga gcgcgtcgcg cagatggaag
     1381 cccgagatcg aacggcccga gcctttgacg agctggccgc tgtcgtcggc gagcgcgaac
     1441 gtgaacgacg gccggtggaa tttctccttc agccgtccgg cgacgatgcc gatcacgccc
     1501 tgatgccagc ccggattgaa cagcgtgatg gtggtcgcgc cgtcggggtt gatcgccgac
     1561 aggtcttcga gcgcctgctg ctgcatgccg gcttcgattt cgcggcgctc gcggttcatc
     1621 gtgtcgagct gctgcgcgag atcccatgcg cggccgatgt cgtcggtggt caggcattcg
     1681 atgccgagcg acatgtccga caggcggccg gccgcgttca ggcgcgggcc gagggcgaaa
     1741 ccgaggtcga agccggacgc gctgcgggcg tcgcgcgcgg ccgcgcgaaa gagggcggcg
     1801 atgcccggct gcatcttgcc tttgcgaatc cgctgcaaac cttgcgcgac cagcacgcga
     1861 ttgttgccgt cgagcttgac cacgtcggcg accgtgccga gcgcgaccag atcgagcagg
     1921 ccgtccagac gcggctcggg gaaagcgtcg ttgaaggcgc cgcggcggcg caattccgcg
     1981 cgtaatgcca gcagcacata aaacatcacg ccgacacccg cgatgcattt gctcgggaat
     2041 gtgcagcccg gctgattcgg attgacgatg gcgcgggcag ccggcagttc gtcgccgggc
     2101 aagtggtgat cggtgaccag cacgtcgata ccgagcgcat tggccgcttc cacaccgtcg
     2161 acgctggcga tgccgttatc caccgtaatc agcagttccg gcttgcccga cgcactgcgc
     2221 gcggccagcg cgacgatctc gggcgtcagg ccatagccgt actcgaagcg gttcggcacc
     2281 agatactcga tctgcccgcc gaacatgcgc agcccgcgca ccgcgaccgc gcaggcggtc
     2341 gcgccgtcgc agtcgtagtc ggccaccacc agcatgcggc gtttgccctg gatcgcgtcg
     2401 gcgagcaacg cagcggcgtt ttcgcaacct ttgagcccgg cgggcggcac gagccgcgcg
     2461 agaccggttt cgatttcatc gggcatgcac acgccgcgcg ccgcgtacag gcgcgcgagc
     2521 accgggtgca ggccgtggcg ggtgaggatg tcggcgtcga cgggtgaggc ggcgcgcgta
     2581 acgattcgag tcattcggaa tggccgtgga tcattcgata aatcattcga tgaagagcga
     2641 cgcgagcacg cgccgtcgcc agaacttgcg caggtcgccg cgcgtgacgg tgagtgtcac
     2701 cgagccggtg tcgccgcaca atgtcaggcc gagctcggcg agctggccgg attgcagcgc
     2761 tgccagcgcc ggtttgaacc agtcggtctg caacgcggcg aaggcgtcgt tccagcgcgc
     2821 ccagtcctgc tcgatgtagg gcgcagagaa gggatcgagt tcgaccaggg tgacgccatc
     2881 gacatgggaa ttcgcatttg cgcttgcgtt gaccgcggat gcgccggccg gagcggcgcc
     2941 gggggcacga cccgaagcac cccccgaagc ggcaccgggg gcatcaccta aagcatcacc
     3001 cgaagcactg cgcgagccgt ttcccgcaac gctagcggcc cgcagccccg caaacgacgc
     3061 cggcggcgca gccgcggcga cgcccgcgct cagagcgaga ccgcgcgtcg ccgccgcgtc
     3121 ggagtacacc tgcgcgaacg ggctcgtcac cggctgtacc gcgccctggg cgtgaaacca
     3181 gatcgagttg acggccggca ggccacgcgc ctcgcgcgct tcattgaccg gatgctcgaa
     3241 ccatgccatc tgtacttcgt tctggagctt catccacgcg cgtgagcgct cgcccgaatg
     3301 ggcctcgtgc ggcagccaga tttcgatgtt gcggccgctc gcgcgcaacg gtgaagcgcc
     3361 cgctagcgtg ccgaaaccat cgcccgacaa gtaccagcgc gccggctttg gcgcttcgat
     3421 gcgtacgccg aggtcctcga tcaacggccg ggcgaccgcc agcagcacac tggcctcgtc
     3481 atcggacagt tcgagcgagg ccggatcgat caacaccaga tggtcgtgcg cgatgcgcac
     3541 atgaaccggt tgcacgcagg cccacgtggc gtggcctggg tcgccgccgt cggcacgcag
     3601 catgtagggc gcaagcggtg c
//
LOCUS       DATASH010000650         3954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_501653,
            whole genome shotgun sequence.
ACCESSION   DATASH010000650 DATASH010000000
VERSION     DATASH010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3954
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501653"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1476
                     /locus_tag="VHV99_17575"
     CDS             <1..1476
                     /locus_tag="VHV99_17575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3637258.1"
                     /translation="VTLIVQPGEIHAVLGENGAGKSTLMKIIYGAVRPDAGEIRWEGQ
                     TVEIANPAAARKLGIGMVFQHFSLFETLTVGENIALALDEPFDLKTLARRIREVSADY
                     GLDIDPQRHVHSLTVGERQRVEIVRCLLQNPRLLIMDEPTSVLTPQAVRKLFETLRRL
                     AAEGCSILYISHKLDEIQELCDTATVLRGGRVTGHVTPKEETHASLAQLMVGHSLPDY
                     TRREHTPGAVLLDVKQLSVESDDPFGTSLNNVSFGVHAGEIFGIAGVSGNGQAELLSA
                     LSGEKRGARANAVTICGKQAGRLGAGARRALGFGFVPEERLGRGAVPAMTLSENALLT
                     AHRQQMVSSGWIKAGAMRAFARRCIEAFDVRCGGSEALAQSLSGGNLQKYIMGREILQ
                     APKVLVVAQPTWGVDVGAAAFIRQQLLDLSARGVAILVISEELEELFDICDRIAVLAG
                     GRLSPVRATGATNAEEIGRWMAGLFGDREGAAPSAEQPAHA"
     gene            1558..2655
                     /locus_tag="VHV99_17580"
     CDS             1558..2655
                     /locus_tag="VHV99_17580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3637259.1"
                     /translation="MILPYRLEARTTPSRTMQLAVPLIAALLTLAIGFLIFSLVGRDP
                     LEAMHAFFIEPLSTVNGWSELLLKASPLCLIGLGLAIGYRANVWNIGAEGQMLLGGIA
                     ASGVAIYFDQATGWWILPTMMIAGVLGGMAWAAIPALLKSRFNTNEILVSLMLTYVAT
                     QLLIYLVSGPWRDPQGMNFPLSEMFSGDALYPTFSGDWHWKWLRGTRLNASVFVTLIA
                     IPCVWLFMRKSFAGYRMNVGGLAPLAARYAGFSDKKTIWTSLLISGGLAGLAGMGEIA
                     GPIGQLQATWSPGYGFTAIIVVFVGRLHPLGIVLASLLMALLYLGGEAVQTSMQLPQA
                     LSGVFQGLLLFCLLGADLFVNYRVRRRSVAA"
     gene            2695..3624
                     /locus_tag="VHV99_17585"
     CDS             2695..3624
                     /locus_tag="VHV99_17585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3637260.1"
                     /translation="MDIQQASALTSSAVTAAIPLMFAGAGELVTEKSGVLNLGVEGMM
                     LMGAVTGYAVTAITGSPWLGVVAAIGAGLAMSLLFAFLTLTMLANQVATGLSLTIFGI
                     GLSAYVGKPYTSAAVRATIDTWTIPGLSKIPVLGPALFSLTPLDYLAFLMFAVIWWFL
                     YRTRAGLVLRSVGESPQVAHSVGFPVVGVRYGAVAFGGGMAGLAGGYYSIVNLHLWQE
                     QLTSGRGWIALALVVFATWRPGRLLIGALLFGAVTGLQFYAQAIGVPVPTQFLAMLPY
                     VATVVVLVLISRNPNTIRLNAPASLGKPFFSAG"
     gene            3704..>3954
                     /locus_tag="VHV99_17590"
     CDS             3704..>3954
                     /locus_tag="VHV99_17590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX3637261.1"
                     /translation="MKRRNLLTAFAWGAASLALAAPLAQTAQAADAPGVAFVYLGNPG
                     DAGWTFAHDQGSKEAEAKFGNKIKITRIENVPESADSERV"
BASE COUNT          635 a         1250 c         1291 g          778 t
ORIGIN      
        1 gtcacgttga tcgtgcagcc cggtgaaatc catgcggtgc tcggcgaaaa cggcgccggt
       61 aaaagcacgc tgatgaaaat catctacggc gcggtgcggc ccgacgccgg cgagattcgc
      121 tgggaaggcc agacggtcga gatcgccaat ccggcggcgg cgcgcaagtt gggcatcggc
      181 atggtgttcc agcatttttc gctgttcgag acgctcacgg tcggcgaaaa catcgcgctc
      241 gcgctcgacg aaccgttcga tctgaagacg cttgcgaggc gtatccgcga agtctccgcc
      301 gactacggcc tcgacatcga cccgcaacgc cacgtgcata gcctgaccgt gggcgagcgg
      361 caacgcgtgg agatcgtgcg ctgtctgctg cagaatccgc gtctgctgat catggacgaa
      421 ccgacttctg tgctgacgcc gcaagcggtc cgcaagctct tcgagacgct gcggcgtctc
      481 gcggccgaag gctgcagcat tctctacatc agccacaagc tcgacgaaat ccaggagttg
      541 tgcgacaccg ccacggtgct acgcggcggc cgcgtgaccg gtcatgtgac gccgaaagaa
      601 gaaacgcatg catcgctcgc gcagttgatg gtcggccatt cgctgccgga ttacacgcga
      661 cgtgaacaca cccccggtgc ggtgctgctc gacgtgaagc agttgtcggt ggaaagcgac
      721 gatccgttcg gcacctcgct caacaacgtc tcgttcggcg tgcatgccgg cgagattttc
      781 ggtatcgcgg gcgtgtcggg caatgggcag gccgaattgc tatcggcact ctccggtgaa
      841 aaacgcggcg cgcgcgccaa cgcggtcacg atctgcggca agcaggcagg gcgccttggc
      901 gcaggcgctc ggcgcgcgct cggcttcggc ttcgtgccgg aagagcgtct gggccgcggc
      961 gctgtgccgg cgatgacgtt gtcggaaaat gcgctgctca ccgcgcatcg tcagcagatg
     1021 gtgagttccg gctggatcaa ggccggcgcg atgcgcgcct tcgccaggcg ttgcatcgaa
     1081 gccttcgacg tgcgttgcgg cggctctgaa gcgttggcgc aaagcctctc cggcggcaac
     1141 ctgcagaaat acatcatggg acgcgagatt ctgcaggcgc ccaaggtgct ggtggtcgcg
     1201 cagccgacct ggggcgtcga cgtcggcgcg gcagcgttca ttcgccagca gctgctcgac
     1261 ttgtccgcgc gcggtgtggc gattctggtg atttccgagg agctagaaga gttgttcgac
     1321 atctgcgatc gcatcgcggt gctcgccggt ggcagacttt cgccggtgcg tgccacgggg
     1381 gcgaccaacg ccgaggaaat cggccgctgg atggcaggcc tgttcggcga ccgcgagggc
     1441 gcggcgcctt cggcggaaca gccggcacat gcctgagccg aagccggatc gaccactttg
     1501 catcggccgc ttcgcagcga cgctaaacac aagaacccgc tataaccaca cgctcccatg
     1561 attcttccgt atcgactcga agcacgcacg acgccctcgc gcacgatgca gcttgccgtg
     1621 ccgctgatcg ccgcgttgct cacgctcgcg atcggcttcc tgatcttcag cctggtcggc
     1681 cgtgatccgc tcgaagccat gcacgcgttt ttcatcgaac cgctttccac tgtgaacggc
     1741 tggtccgaac tgctgctgaa ggcctcgccg ttgtgcctga tcggcctcgg tctcgcgatc
     1801 ggctatcgcg ccaacgtctg gaatatcggc gctgaagggc agatgctgct tggcggcatc
     1861 gccgcgagcg gcgtcgcgat ctatttcgat caggccaccg gctggtggat tctgccgacc
     1921 atgatgatcg ccggcgtgct cggcggcatg gcgtgggcgg cgatccccgc gctcctgaaa
     1981 agccgcttca acaccaacga gattctggtc agcctgatgc tcacgtatgt ggccacgcag
     2041 ttgctgatct acctggtgag cggcccgtgg cgcgatccgc aggggatgaa ttttccgctc
     2101 tccgagatgt tcagcggcga cgcgctctat ccgacgttct ccggcgactg gcactggaaa
     2161 tggctgcgcg gcacgcgttt gaacgcctcc gtgttcgtca cgctgatcgc gattccatgc
     2221 gtgtggctgt tcatgcgcaa gagctttgcg ggctaccgca tgaacgttgg cggtctcgcg
     2281 ccgctcgccg cgcgctacgc cggtttctcc gacaagaaaa ccatctggac gtcgctcctg
     2341 atcagcggcg gtctggcggg tctcgccggc atgggcgaga tcgcgggtcc gattggccag
     2401 ttgcaggcga cgtggtcgcc gggttacggt tttaccgcga tcatcgtcgt gttcgtgggc
     2461 cggctgcatc cgctcggcat cgtgctcgcg agcctcctga tggcgctgct gtatctgggc
     2521 ggcgaagcgg tgcagacctc gatgcaattg ccgcaggcgc tctccggcgt gttccagggg
     2581 ctattgctgt tttgcctgct gggtgctgac ctgttcgtga actatcgcgt gcgccggcgc
     2641 tccgtcgccg cgtaagtcca ctcattcgcc actgattttt cccgccggat ctccatggat
     2701 attcaacaag ccagcgcgct cacctcgagc gccgtcaccg ccgcgattcc gctgatgttc
     2761 gcgggcgcgg gcgaactcgt caccgagaag tcgggcgtgc tcaacctcgg cgtcgaaggc
     2821 atgatgctga tgggcgccgt gaccggctat gcggtcaccg ccattaccgg cagcccttgg
     2881 ctcggggtgg tcgccgcgat cggcgcgggc ctcgcgatgt cgctgctatt cgcgttcctc
     2941 acgctgacca tgctcgccaa tcaggtcgcc acgggcttgt cgctgacgat cttcggcatt
     3001 ggtttgtcgg cctatgtcgg caaaccgtac acgtcggcag cggtgcgcgc cacgatcgac
     3061 acatggacca ttccgggtct ctcgaagatt ccggtgctgg gacccgcgct cttcagcctc
     3121 acgccgctcg attacctcgc gttcctgatg ttcgcggtga tctggtggtt cctgtatcgc
     3181 acgcgtgcgg gcctcgtgct gcgctcggtc ggcgaatcgc cgcaagtggc gcattcggtc
     3241 ggctttccgg tggtcggcgt gcgttacggc gcggtcgcct tcggcggcgg catggcgggg
     3301 ctcgcaggcg gttactactc gatcgtcaat ctgcatttgt ggcaggagca gctgacgtcc
     3361 ggccgcggct ggatcgcgct cgcgctggtg gtgttcgcga cgtggcgccc ggggcgcctg
     3421 ctgatcggcg cgctgctgtt cggcgccgtg accggtttgc agttttacgc gcaggccatt
     3481 ggcgtgccgg tgccgacgca gttcctcgcg atgttgccgt atgtcgcgac cgtcgtcgtg
     3541 ctggtgctga tttcccgcaa cccgaacacg attcgtctga atgcacccgc atcgctcggc
     3601 aagccgtttt tctcggcggg ctgacgtgga tgggttcact agcctatccg cacgtgacca
     3661 accacacgtt tcaaatcaat tgacaaacac aacaggagaa aacatgaaga gaagaaatct
     3721 gctgaccgca ttcgcatggg gcgcggcctc gctggcgctt gccgcgccgc tcgcgcaaac
     3781 cgcgcaagcg gctgacgcgc cgggcgtcgc attcgtctat ctcggcaatc cgggcgacgc
     3841 gggctggact ttcgcgcacg atcagggctc gaaggaagcg gaagcaaagt tcggcaacaa
     3901 gatcaagatc acccgcatcg agaacgtgcc ggaatcggcc gactcggaac gcgt
//
LOCUS       DATASH010000651         3423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5052932,
            whole genome shotgun sequence.
ACCESSION   DATASH010000651 DATASH010000000
VERSION     DATASH010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5052932"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..138)
                     /locus_tag="VHV99_17595"
     CDS             complement(<1..138)
                     /locus_tag="VHV99_17595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006476792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEX3637262.1"
                     /translation="MHCPFCRHADTQVVDSRVSEDGATIRRRRRCPACDKRFTTYERV
                     EL"
     gene            complement(145..1392)
                     /gene="glyA"
                     /locus_tag="VHV99_17600"
     CDS             complement(145..1392)
                     /gene="glyA"
                     /locus_tag="VHV99_17600"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX3637263.1"
                     /translation="MFDRAQSTIANVDPELWKVIEQENRRQEEHIELIASENYTSPAV
                     MAAQGSQLTNKYAEGYPGKRYYGGCEYVDVAEQLAIDRVKQLFGAEAANVQPNSGSQA
                     NQGVFFAMLKPGDTIMGMSLAHGGHLTHGSPVNMSGKWFNVVSYGLNEAEDIDYDAAE
                     KLAQEHKPKLIVAGASAFSLRIDFERMSKIAKSVGAYFMVDMAHYAGLVAAGVYPNPV
                     PHADFVTTTTHKSLRGPRGGVILMKAEFEKQINSAIFPGIQGGPLMHVIAGKAVAFKE
                     ALSPEFKAYQQQVVENARVLAETLVKRGLRIVSGRTESHVMLVDLRAKKITGKAAEAA
                     LGAAHITVNKNAIPNDPEKPFVTSGVRLGSPAMTTRGFGIKEAEQVGNLIADVLDNPE
                     DAATIERVRAQVAELTQRFPVYR"
     regulatory      complement(1559..1667)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01750"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ZMP/ZTP riboswitches appear to regulate genes
                     involved in one-carbon metabolism; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="5-aminoimidazole-4-carboxamide
                     ribonucleotide (ZMP)/
                     5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl
                     5'-triphosphate (ZMT)"
                     /db_xref="RFAM:RF01750"
     gene            1906..2652
                     /gene="ydfG"
                     /locus_tag="VHV99_17605"
     CDS             1906..2652
                     /gene="ydfG"
                     /locus_tag="VHV99_17605"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013696778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HEX3637264.1"
                     /translation="MIVFVTGASAGFGAAIARAFVKGGHRVVATARRKDRLQALADEL
                     GDALLPFELDVRDRAAVEAVPASLPADFAALDVLVNNAGLALGIEPAQKANLDEWNTM
                     IDTNCTGLVQVTHALLPGMVERNRGHIFNLGSAAGRWPYAGGNVYGATKAFVRQFSLN
                     LRADLTGTALRVTDIEPGLCGGTEFSNVRFRGNDEKAAKMYENVQPLTPEDIADSIYW
                     IATRPAHVNINTIELMPVAQSFSALSVHRG"
     gene            2735..3214
                     /gene="ybgC"
                     /locus_tag="VHV99_17610"
     CDS             2735..3214
                     /gene="ybgC"
                     /locus_tag="VHV99_17610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HEX3637265.1"
                     /translation="MRGMNMSTIQPGTEIGFTWPIRVYYEDTDAGGIVFYANYLKFFE
                     RARTEWLRACGVDQNRLASEMGAIFIVRSTAVDYRAPARLDDVVNIVSRIERLGRASV
                     DFAQEAWRDGTLLATGSVRVGCVERIALRPAAIPPPVLAALRRGPNVNDGGVSTDND"
BASE COUNT          609 a         1081 c         1053 g          680 t
ORIGIN      
        1 cagctcgacc cgctcatacg tcgtgaaacg cttgtcgcag gccgggcagc ggcggcgccg
       61 gcgaatcgtc gcgccgtctt cggatacgcg cgagtcgacg acctgcgtat cggcgtgacg
      121 gcagaaggga cagtgcatgg cggcttagcg gtaaaccggg aagcgctggg tcaactcggc
      181 gacttgcgcg cgcacacgtt cgatcgtggc ggcgtcttcc gggttgtcca gcacgtcggc
      241 gatcaggtta cccacctgct ccgcttcctt gatgccgaag ccacgcgtgg tcatggccgg
      301 cgagccgaga cgcacaccgc tcgtgacgaa cggcttttcc gggtcgttcg ggattgcgtt
      361 cttgttgacc gtgatgtgtg ccgcgccgag cgcagcttcc gctgccttgc cggtgatctt
      421 cttggcgcgc aagtcgacca gcatcacgtg gctttcggta cggcccgaga cgatgcgcag
      481 gccgcgcttg accagcgttt cagccagcac gcgcgcattc tcgacgactt gctgctgata
      541 tgccttgaac tccggcgaca gtgcttcctt gaacgcgact gccttgccgg cgatcacgtg
      601 catcagcgga ccaccctgaa tgcccgggaa gatggccgag ttgatctgct tttcgaactc
      661 ggctttcatc aggatcacac cgccgcgcgg gccgcgcagg ctcttatgcg tggtggtggt
      721 gacaaaatcg gcgtgcggta ccgggttcgg atagacgccc gcggcgacga ggccggcata
      781 gtgcgccatg tcgaccatga aatacgcacc caccgacttc gcgatcttcg acatccgctc
      841 gaagtcgatg cgcagcgaaa acgccgacgc gccggccacg atcagcttcg gcttgtgttc
      901 ctgtgcgagc ttctcagccg cgtcgtagtc gatgtcttcg gcttcgttca ggccatagct
      961 caccacattg aaccacttgc ccgacatgtt gaccggcgaa ccgtgagtca ggtggccgcc
     1021 gtgcgcgagg ctcatgccca tgatcgtgtc gcccggcttg agcatggcga agaacacgcc
     1081 ctggttcgcc tgcgaaccgg agttcggctg cacgttggcg gcttcggcgc cgaacagttg
     1141 cttgacgcgg tcaatcgcca gttgctcggc gacgtcgaca tattcgcagc cgccgtagta
     1201 gcgcttgccc ggataccctt cggcgtattt gttggtgagt tgcgagcctt gcgcagccat
     1261 cacggccggg ctcgtgtaat tttcagacgc gatcagttcg atgtgctctt cctgacggcg
     1321 gttttcctgc tcgataacct tccagagttc aggatcgacg ttggcgatgg tgctttgggc
     1381 tctgtcaaac atacggattc cgttgagtgt gttcaggttg accggatcgt gcgccgaatc
     1441 ttgcgtagag ggtacgcagc gctgctggcg gctgggccag ccctgacgtg gcggatggag
     1501 agtcgccgca cacgcgaggc aggacagcca ccgtcagcgc agatcaatgc gcgcggatca
     1561 cggctgccca ggcgaacggc aaaacggctc cctgcgcttc acggtgggtt gttccacctt
     1621 gaacccgatt gcttgaatcg gttctatcgc cagtcacgca gggatgagcg cgttagttta
     1681 ttggaagagg atcgaatagg caaccggctt cctgccgcgc cggcgggcgc tcacgcagcc
     1741 ggttatcgcg gcgccgcaaa cagcgcgcaa acatctgatg aaaacgcacc tgccacccgc
     1801 tccgcgtccc tgccgcaacc ccttgtgcgc ctgcttcatc cttgccgcag cgccgcattg
     1861 gaagtaggct tggggccttt ctcatttacc agccaggaaa ctgcaatgat cgtatttgtc
     1921 accggagcgt ccgcgggatt cggcgccgct atcgctcgcg ccttcgtcaa gggcggccat
     1981 cgggtcgtcg ccaccgcgcg ccgcaaagac cgcctgcagg cacttgccga cgaactcggc
     2041 gacgcgctcc tgccgtttga gctcgacgtg cgtgaccgcg ccgccgtcga agccgtgccg
     2101 gcctcgctgc ccgccgactt cgccgcgctc gacgtgctcg tcaataacgc cggcctcgcg
     2161 ctcggcatcg agccggcgca aaaggcgaat ctggacgaat ggaacaccat gatcgacacc
     2221 aactgcacgg gcctcgtgca ggtcacgcat gcgctcctgc ccggaatggt ggagcgtaac
     2281 cgtgggcaca ttttcaatct ggggtcggcg gccggccgct ggccgtacgc gggcggcaac
     2341 gtatatggcg ccaccaaggc gttcgtacgc cagttcagcc tgaatctacg cgccgatctg
     2401 accggcacag ccttgcgcgt gaccgacatc gagccgggcc tgtgcggcgg caccgagttc
     2461 tcgaacgtgc gctttcgcgg gaacgacgag aaagcggcca aaatgtacga aaacgtccaa
     2521 ccactgacgc ccgaagatat cgccgactcg atctactgga tcgccacccg gccagcccac
     2581 gtcaacatca acacgatcga gctgatgccg gtggctcaat ccttctccgc tttgtcggtt
     2641 catcgcggct gaaattccgg ctgaaattcc ggttgaggca agccaggcgg ggcgcacatc
     2701 ttgaaacaga cctcagggta tgcgttccgg taaaatgcgc ggcatgaata tgtcgaccat
     2761 ccagcccggt acggaaatcg gcttcacgtg gccaatccgc gtgtattacg aagataccga
     2821 cgccggcggc atcgtgtttt acgcgaatta cctgaagttt tttgagcggg cgcgcaccga
     2881 atggctgcgc gcgtgcggcg tcgaccagaa caggctcgcc agcgaaatgg gggccatttt
     2941 catcgtgcgc agcaccgcag tcgattatcg ggcgccggcc cgcctcgacg atgtcgtcaa
     3001 catagtgagc cgaatcgagc gtcttggccg cgcttccgtc gattttgcac aggaagcgtg
     3061 gcgcgacggc acgttgcttg ctaccggttc cgtccgggtc ggctgcgttg agcgcattgc
     3121 gctgcggcca gccgcgattc ctccgccggt tctcgctgcg ttgcggcgcg ggccgaacgt
     3181 gaatgacggc ggcgtgtcaa cggacaacga ctgaatcccg ttgttacggg gccagtgaac
     3241 tcgtatcgat caagcaacac aaagcatggt tcggcttgca atatcgccga tgcttccgtt
     3301 ttgctcacgg caagcttcga gtggccttgc agccggacgc ccccttccgg gacgttaaaa
     3361 cgaaccttta tgaacactac acaagatctg tcgatcgttt ctctcgtact caatgcgagc
     3421 ctg
//
LOCUS       DATASH010000652         3718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1294070,
            whole genome shotgun sequence.
ACCESSION   DATASH010000652 DATASH010000000
VERSION     DATASH010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294070"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="VHV99_17615"
     CDS             <1..791
                     /locus_tag="VHV99_17615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3637266.1"
                     /translation="RVLLCVHGLTRSGRDFDRLAAQFAGTYRVVCPDVAGWGLSSWLS
                     NPNLYGVPQYVADMITLIARLNVETVDWFGTSMGGLIGLALAGLPETPIRKLLLNDVG
                     PHLEPAAVQRIGDYVGKPVRFETLQQGIDYAALLAQTFGPLTPEEWREINTPLLHQQG
                     GEWLFRYDPRIAVPFAATTEEAAKLGEAVLWQSLAAFPGPVLVVRGEQSDLLSRETVA
                     RMVESGRAVSSAEIAGVGHAPAFLSADQIDLARQFFIGPAADAS"
     gene            893..1246
                     /locus_tag="VHV99_17620"
     CDS             893..1246
                     /locus_tag="VHV99_17620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX3637267.1"
                     /translation="MAVIRHHVGKRLSEIAVHNGTVYLAGQIAEDTDQDITGQTREVL
                     GHIDRLLGEVNSDKSQLLSVQIYISDMVHFAGMNAVWDEWVAQGATPPRATVEARLAN
                     PKCLVEIVVVAAQRD"
     gene            1309..3552
                     /locus_tag="VHV99_17625"
     CDS             1309..3552
                     /locus_tag="VHV99_17625"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEX3637268.1"
                     /translation="MTTEIVTSTPTPLPSFDEAMAFVREHAGEVRLSSGELLADHAAG
                     TASIMRTLNVDPPAVLAAALFALTPHLRDPERVIADNFGEEVAQLVGDVRKLLRLGTV
                     SMRAAQNAIPEAGRDAQAARRAQVEALRKMLLAFAQDIRVVLIRLASRLQSLRYYAAA
                     KVTPSPDVARETLDIYAPLANRLGIWQLKWELEDLALRFEEPVTYKRIARLLDEKRVE
                     RESYVAQAIERLQRELAAAHVQAEVSGRPKHIYSIWRKMRGKELDFAELYDVRAFRVI
                     VPDIKDCYTVLGIVHNLWQPVPKEFDDYISRPKPNGYKSLHTVVIGDDGRAFEVQIRT
                     QEMHQFAEYGVAAHWRYKEAGTRGYGGQFSANEKYDEKIAWLRQLLAWKDEVSEGEHG
                     EKRAAQPWEQLRQATLDDDHIYVLTPQARVIPLPHGATPVDFAYHLHSELGHRCRGAR
                     VDGAMVPLNTPLQNGQTVEIVAVKEGGPSRDWLNPQLGYLQSHRARQKVRAWFNAVEV
                     QEHIASGRAMVEKTLQREGKTSVNLDQLAAKLGFKTTDDLFSVVGKEEFSLRLVEQAL
                     HDAPPPEPVVEAPEQFEKRSSGASVARGASTGVLVVGVDALLTQLARCCRPAPPDDIC
                     GFVTRGKGMSIHRSDCPTFLRMADRAPERVLQTAWSADVMSGRGQSVYPVDLSIEATD
                     RQGLLRDISEVFAREKMNVIGVKTQSRRNAAFMQFTVEVSNAAQIQRACTLLGEVTGV
                     MRASRKS"
BASE COUNT          691 a         1098 c         1241 g          688 t
ORIGIN      
        1 cacgtgtgct gctgtgcgtg cacggcttga cgcgctcagg gcgcgatttc gaccgcctcg
       61 cggcgcaatt cgccggcact taccgcgtgg tgtgtccgga tgtggcgggg tggggcttgt
      121 cttcgtggct cagcaacccc aacttgtatg gcgtgccgca atacgtcgcc gacatgatca
      181 cgttgatcgc gcgcctcaac gtcgaaacgg tcgactggtt cggcacctcg atgggcggtc
      241 tgattggact tgcgctggcc ggcctgccgg aaacgccgat tcgcaagctg ctgctgaacg
      301 acgtcgggcc gcatttggaa ccggcggccg tgcagcggat cggcgattac gtcgggaaac
      361 ccgtgcgctt cgaaacgctg cagcagggca tcgattacgc tgcgttgctg gcgcaaactt
      421 ttggcccgct cacaccagag gagtggcgcg agatcaatac accgctcttg catcagcaag
      481 gcggcgaatg gctgttccgc tatgacccac ggatcgccgt gccgtttgcc gcgaccaccg
      541 aagaggcggc caagctcgga gaggccgtgc tgtggcagtc gctggccgcg tttccggggc
      601 ctgtgctggt ggtgcgcggt gagcagtcgg atctgctttc tcgtgagacg gtcgccagga
      661 tggtcgagag cgggcgcgcc gtatcgagcg cggaaatcgc gggcgtgggg catgcgccgg
      721 cgtttctgtc ggccgatcag atcgacctcg cgaggcagtt ctttatcggg ccggccgctg
      781 acgcgtcata atatcgaggt ccgcgcggcg tccgtgagcc gtgagccttg ggcgagcgga
      841 cgcaatgcgg gacgcatctt cgatcaattt tcaaccaacc tcaggattct tcatggcagt
      901 cattcgtcac cacgtcggca aacgcctctc ggagatcgcc gtgcacaacg gcaccgtgta
      961 cctcgccggt cagattgctg aagatacgga tcaggacatc acgggtcaaa cgcgcgaagt
     1021 gctcggccac atcgaccgtt tgctgggtga agtgaacagc gacaagtcgc agttgctgtc
     1081 cgtgcagatc tacatctcgg acatggtgca tttcgccggt atgaatgccg tgtgggacga
     1141 gtgggtcgca caaggcgcca cgccgccgcg cgctactgtg gaagccaggc tcgcgaaccc
     1201 gaagtgcctc gtcgagatcg ttgtagtcgc tgcgcagcgc gattaagccc ggctcgcagt
     1261 tgttgttgaa attccgttga tccgtctggc ccggtgggcc gtcgcaccat gactaccgaa
     1321 atcgttacga gcacgccgac acccctgcct tccttcgacg aagcgatggc gttcgtgcgc
     1381 gaacatgcgg gcgaggtacg gctctcgtcc ggcgagttgc tggcggatca tgcggcgggt
     1441 acggcgtcga tcatgcgcac gctcaacgtc gatccgccgg ctgtgctggc ggcggccttg
     1501 ttcgcgttga cgccgcatct gcgagacccg gagcgtgtga tcgccgacaa cttcggcgaa
     1561 gaagtcgcgc aactggtcgg cgatgtgcgc aagttgctgc gcttgggcac cgtgagtatg
     1621 cgtgccgcgc aaaatgcgat tcccgaagcc gggcgagacg cacaagccgc acgccgcgcc
     1681 caggtcgagg cgctgcgtaa gatgctgctt gcgttcgcgc aggatattcg ggtggtgttg
     1741 atccggctcg cgtcgcgctt gcaatcgttg cgttattacg cggcggcgaa agtcacgccg
     1801 tcgcccgacg tcgcgcgtga gacgctcgat atttacgcgc cgctcgcgaa ccgtctgggc
     1861 atctggcaac tgaaatggga gctcgaggat ctcgcgctcc gtttcgaaga accggtcacg
     1921 tacaagcgca tcgccaggct gctcgacgag aagcgcgtcg agcgcgagag ttatgttgcg
     1981 caggcgatcg agcgcctgca gcgcgagttg gccgcggcgc atgtgcaggc cgaagtcagc
     2041 ggccgaccca aacacatata cagcatctgg cgcaagatgc gcggcaagga gctggacttc
     2101 gccgaactgt acgacgtgcg tgcgtttcgc gtcatcgtgc cggacatcaa ggattgctac
     2161 acggtgctcg gcatcgtgca caacctgtgg cagccggtac cgaaagagtt cgacgactac
     2221 atctcgcggc cgaagccgaa cggctataaa tcgctgcaca cggtcgtgat cggcgacgac
     2281 ggccgcgcat tcgaagtgca gatccgcacg caggagatgc atcagttcgc cgagtacggc
     2341 gtggcggcgc actggcgcta caaggaagcg ggcacgcggg gttacggcgg ccagttcagc
     2401 gccaatgaga agtacgacga gaagatcgcg tggctgcgtc agttgctcgc gtggaaagat
     2461 gaagtctcgg aaggcgagca cggcgagaaa cgcgcggcgc agccatggga gcaactgcgc
     2521 caggccacgc tcgacgacga ccacatctac gtgctcacgc cgcaagcacg cgtgattccg
     2581 ctgccgcacg gcgcgacacc ggtggacttc gcatatcacc tgcatagcga gttagggcat
     2641 cgctgccgtg gcgcgcgtgt cgacggcgcg atggtgccgc tcaacacacc actgcagaac
     2701 ggccagacgg tcgagatcgt cgcggtgaag gaaggggggc cgtcgcgtga ttggctcaac
     2761 ccgcagcttg gatatctgca gagccatcgg gcgcgccaga aagtgcgcgc gtggttcaac
     2821 gcggtcgaag tgcaggagca cattgcaagc ggccgggcga tggtcgaaaa gaccttgcag
     2881 cgcgaaggca agacttccgt caatctcgat cagctcgccg cgaagctcgg cttcaagact
     2941 accgacgatc tgttctcggt ggtcggcaag gaagaattca gtctgcggct cgtcgagcag
     3001 gcgctgcacg atgcgccgcc gcccgagcct gtcgttgaag cgcctgagca gttcgagaag
     3061 cgcagcagtg gcgcgagcgt tgcacgcggg gcttccacgg gtgtgctagt ggtcggcgtc
     3121 gacgcgttgc tcacgcaact cgcgcgttgt tgccgtcccg cgccgcccga cgatatctgc
     3181 gggtttgtca cgcgcggcaa gggcatgtcg attcatcgca gcgattgccc gaccttcctg
     3241 cgcatggccg atcgcgcgcc ggagcgtgtg ctgcaaaccg catggtcggc agatgtcatg
     3301 agcggccgcg ggcaatcggt ctatcccgtc gacctcagca tcgaggcgac agataggcag
     3361 ggcctgttgc gcgatatctc cgaagttttc gcgcgcgaga aaatgaatgt gatcggcgtg
     3421 aaaacacaat cgcgtcgtaa cgccgcattc atgcagttca ctgtcgaggt atcgaatgcc
     3481 gctcaaattc agcgcgcgtg tacgttgctt ggtgaagtaa caggcgtgat gcgcgcttcg
     3541 cgaaaaagtt gacgcacgga cgaggtggcg ggtgccagcg gccattcgtt caaagcgcgc
     3601 gagcgcgtgc gcgggaattt aaagtcggtg cataaaagta cttgccaagc ggtgtggggc
     3661 tccatataat ctcgtttctt caggctcgta gctcagctgg ttagagcacc accttgac
//
LOCUS       DATASH010000653         2089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2084737,
            whole genome shotgun sequence.
ACCESSION   DATASH010000653 DATASH010000000
VERSION     DATASH010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2084737"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            239..625
                     /locus_tag="VHV99_17630"
                     /pseudo
     CDS             239..625
                     /locus_tag="VHV99_17630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922355.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
     gene            855..1067
                     /locus_tag="VHV99_17635"
     CDS             855..1067
                     /locus_tag="VHV99_17635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016347319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637269.1"
                     /translation="MKSILVVADIPYDPLAQSDVATLTHAQMQRIGGGRSVVVTVDGG
                     PAKTTVNDFDINNAIWEGRIAGPMIN"
     gene            complement(1528..1920)
                     /locus_tag="VHV99_17640"
     CDS             complement(1528..1920)
                     /locus_tag="VHV99_17640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637270.1"
                     /translation="MNQDNDELPVMEYDWGDKSIRQIAENIWHAQKAGWPSVLTYVYR
                     SKQDKYLIRKESLRNIPRIQSRDEYPFASTLENEGSVWIGHASVSQQNAQRDLMTRFY
                     QLNKAYVKGTTLRFRVSVINYPVDQVKR"
BASE COUNT          460 a          614 c          566 g          449 t
ORIGIN      
        1 gttccagact cctaccctgg gtctggcgaa ccgcgattct agcgtttaca tccgccttca
       61 aacaaaagcc gcgtcgtgtg aatacatcat gtgaatacaa tttcggtccg agtcattccc
      121 ggttgttcaa ttcgtgcttt agctggcagc ggtcgcacga aagagtggac gcgacgcccc
      181 gtcggtaagc gccaaagtta atcggatcgg cgctttcgac gcggcagcgg ccagtgacat
      241 gggattgttc gcgctacagc cgatcgccgc aggcgagcgg gtcatcgagt acaagggaga
      301 actgaccagc aggcggcacg ccgccgtccg tcaacgcgcc aaggctggcc acacgttcgt
      361 attcggcctg tctgacggac gtgtggtcga cggcagccgt ggtggcaaca gcgcccgctt
      421 tctgaatcac gccagcgccc ctaattgcaa ggccatcgag acgggcgacc gggtcttcat
      481 tcatgcgctc gcccctatcg cagccggcga tgagctttat atcgactacg gtctgtcagt
      541 cgacggtgag atcaccgacg acattcgtgt ccggtacacg tgtcattgcg gcacgtcagc
      601 atatcgccga accattctgg gctctttctc caacaacaca gcgcatacct gttcgtgggc
      661 atccaacata caggccgtgg aggtcgtaag caggtgcgcc cggaacatgt gcacgggccc
      721 atgaatcaag gcctcggcgc attcgtacgt gcgctcgcgg atattggcat ggcccttgca
      781 ataacccgag cgagcatgtg ccgtgctcat ccagatccca cgacatttcg taccctatag
      841 cccggagaaa catcatgaag agcattctcg tcgtcgccga cattccgtac gatccgctcg
      901 cacaaagcga cgtagccaca ctcacgcacg cgcaaatgca gcggatcggc ggcggacgca
      961 gtgtcgtcgt cacggtcgat ggcggtcccg ccaagacgac cgtcaacgat ttcgacatca
     1021 acaacgctat ctgggaaggg cggatcgcgg gtccgatgat caactgaaac gcatcctgtt
     1081 ttcattactg atgtcccttc gcgcgtgagc gggaagggac atcggcattc atgctgccgc
     1141 gcgctgcctc gaagtgcgcc ggagcattcg acgttgccga aggcaagaca ggccgatggt
     1201 cactcattcg cgacgcagac tgcttggtcc gatttgcctc atgcgcgcta tgggtcagaa
     1261 aatatgcgcg cgccaggggc gaagcctaac aattgcggcc gatatggcac gaacgacagc
     1321 aaaagttcct gcaactgcct gaccaacagt cattcacgag tgcccgcagt ggccgaactc
     1381 gagtcgttgc gcgcacaact gccgctaccc gcccgtcggc cgaagccgac ggtgggcgat
     1441 caggaacgtg cggccgttcc tgatctcttt gaacggaact gtcgcccata gagcgatcat
     1501 ctagccgatc ccgtagttaa tttaacgtta ccgtttcact tggtcgacgg gatagttaat
     1561 cacgctcacc ctgaatctca gggtggttcc tttgacataa gccttgttga gctgatagaa
     1621 acgggtcatc aagtctcgct gtgcgttttg ttgcgaaact gaggcatggc caatccacac
     1681 gctgccttca ttctctaacg tactggcgaa agggtattcg tcacgactct ggattctcgg
     1741 gatgttacgc agcgattctt ttcgaattaa atatttatcc tgttttgacc ggtaaacata
     1801 ggtcagaacg cttggccaac ctgccttctg ggcatgccat atattctccg caatttgccg
     1861 aatactcttg tccccccagt catactccat taccggaagt tcgtcgttat cctgattcat
     1921 gcaaacgcta cctcctggag gtgaaatacc catacaagaa taactagcga tggatccaag
     1981 ttgacgatag tcgaatgcgc caaaacccgc aagccgggcg ccgagtcggt gtatcgatac
     2041 ggcgcttcaa ctataagccg actgtccgca acggccgatg aagcgatgt
//
LOCUS       DATASH010000654         1694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1690187,
            whole genome shotgun sequence.
ACCESSION   DATASH010000654 DATASH010000000
VERSION     DATASH010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1690187"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VHV99_17645"
     CDS             <1..543
                     /locus_tag="VHV99_17645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3637271.1"
                     /translation="SARDLGVEPKRVSEEALAYLASLPFPGNVRQLENLANWLTVMAP
                     AQTIEIKDLPPDLGPTQAGVTDLSNGGNGAIGTGEAGLAGGAPINGTSAATHPATAAG
                     MPAATTVSAWEGGLRTEVARMLRENAADVMDELARRFEAAVIREALDFTRGRKVEAAE
                     RLGIGRNTITRKIQELNLEP"
     gene            730..>1694
                     /locus_tag="VHV99_17650"
     CDS             730..>1694
                     /locus_tag="VHV99_17650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX3637272.1"
                     /translation="MPASPAPFSWPLSPDPFLSLEVLDDPGALAWVEAQNARTRAAWG
                     SSAEFESLRLRLADAYLPRERPVIPDRWKEWAYDLWQDERNPKGIWRRTTWAAWRSGA
                     PVWQNLLDFDALGAAEGTPWVCVELDILYPDGDRALITLSPGGSDALVVREFDIDAQR
                     FVDDGFVIAKAGKHTASWIDRDTLYVGWDNGRKTLTRSGYPREVRRWTRGSALAEAPV
                     VFKGAFGDIGVEAHYDPVERRHTVVSSVDFFDSHTYYLDGADADAGDAGNRATAASNG
                     SNAYNANNASAWQQYDVPSYVAVGGWQGWLLLEPRLDWDCNGVRYP"
BASE COUNT          276 a          531 c          579 g          308 t
ORIGIN      
        1 agtgcgcgcg atctgggcgt ggagccgaag cgggtgtccg aagaggcgct ggcttatctg
       61 gcatcgctgc cgtttcccgg caacgtgcgg caactggaga atctcgccaa ttggctcacg
      121 gtgatggcgc cggcgcagac catcgagatc aaggacttgc cgcccgatct ggggcccacg
      181 caagcaggcg tgaccgatct ctcaaacggt ggcaatggcg cgatcggcac gggtgaggcg
      241 ggtctcgccg gcggtgcgcc gatcaacggc accagcgcgg ccacgcatcc ggctaccgct
      301 gccggcatgc cggcagcgac caccgtgagc gcctgggaag gcggcttgcg taccgaagtc
      361 gcgcggatgt tgcgtgagaa tgctgccgac gtcatggacg aactcgcgcg gcgcttcgaa
      421 gcggcggtga ttcgcgaggc gctggatttc acacgcggac gcaaggtcga ggcggcggag
      481 cggctcggca tcggtcgcaa tacgattacg cgcaagattc aggaactcaa tctcgagccg
      541 taaggttggc ggggtggctt gccagccgcc ccgccagttt cactcgttgg gccgccggtt
      601 gggcaggtct cccagacagt cccgtcaacc gccccgtcgg ctactctttc gctgttcccg
      661 cgcgtagcgt caaaggccct cggcctcgcg caaggcataa tcaacgctgt tttcattcga
      721 cagctgacga tgcccgcttc tcccgctcct ttctcctggc cgctttcccc cgatcccttt
      781 ctgtcgctcg aagtgctcga cgaccccggc gctctcgcgt gggtcgaggc gcaaaacgcc
      841 cgcacgcgcg ccgcgtgggg cagcagcgcg gaattcgagt cgctcaggct gcggctcgcc
      901 gacgcttatc tgccgcgcga gcgtccggtc attcccgatc gctggaagga gtgggcctac
      961 gatttgtggc aggacgaacg caatcccaaa ggcatctggc ggcgtaccac atgggcagcg
     1021 tggcgcagcg gggcgcctgt ctggcagaac ctgctcgatt tcgacgcgct cggtgcggcc
     1081 gaaggcacgc catgggtttg cgtcgagctc gacattcttt atccggacgg cgatcgtgcg
     1141 ctgatcacgc tgtcgccggg cggctcggat gcgctcgtgg tgcgcgagtt cgacatcgac
     1201 gcgcaacgtt ttgtcgacga tggtttcgtg attgcgaagg caggcaagca cacggcttcg
     1261 tggatcgatc gggatacgct ttatgtcggc tgggacaacg gccgcaagac gctgacgcgc
     1321 tccggctatc cgcgcgaagt gcggcgctgg acgcgcggca gtgcgctggc cgaggcgccg
     1381 gtggtcttca agggcgcgtt cggcgatatc ggcgtggagg cgcactacga tccggtcgag
     1441 cggcgccaca cggtggtgag cagcgtcgat ttcttcgatt cgcatacgta ttaccttgat
     1501 ggtgccgatg ctgatgcggg cgacgcgggc aatcgcgcga ctgctgccag caacggcagc
     1561 aacgcttaca acgctaacaa cgccagtgcc tggcaacagt acgacgtacc gtcatacgtc
     1621 gcggtcggcg gatggcaggg ttggctgctg ctcgagccgc gcctcgactg ggattgcaac
     1681 ggtgtgcgct atcc
//
LOCUS       DATASH010000655         9189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6831619,
            whole genome shotgun sequence.
ACCESSION   DATASH010000655 DATASH010000000
VERSION     DATASH010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 9189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9189
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6831619"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VHV99_17655"
     CDS             <1..331
                     /locus_tag="VHV99_17655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF520 family protein"
                     /protein_id="HEX3637273.1"
                     /translation="DFKLGQVKDVLLSKMAKRNVDVRFLDYGKIEKIGGDKVKQIIKI
                     KKGVSGDLSKKIVRLVKDSKIKVQASIQGDAVRITGGKRDDLQSVIAMLRKDVTDTPL
                     DFNNFRD"
     gene            complement(731..1375)
                     /gene="plsY"
                     /locus_tag="VHV99_17660"
     CDS             complement(731..1375)
                     /gene="plsY"
                     /locus_tag="VHV99_17660"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEX3637274.1"
                     /translation="MQNLIVAVVAYLIGSVSFAVIVSAAMGLDDPRSYGSGNPGATNV
                     LRSGSKKAAILTLIGDAFKGWLPVWFVVHFGTRYGLDDTSVAIASVAVFLGHLYPIFF
                     RFKGGKGVATAAGVLIAINPILGVATLLTWLIVAFFTRYSSLAALAAAVFAPIFDGFL
                     FGPHIIALAIVVMSSLLVWRHRGNIAKLMHGQESRIGAKKKTDAAASAAGGNEP"
     gene            complement(1489..1980)
                     /gene="ybaK"
                     /locus_tag="VHV99_17665"
     CDS             complement(1489..1980)
                     /gene="ybaK"
                     /locus_tag="VHV99_17665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEX3637275.1"
                     /translation="MSKSRHVSETPATQFLRRHGVTFGEHPYDYVEHGGTEESARQLG
                     VDEHHVVKTLVMEDEHAKPLIVLMHGDRTVSTKNLARQIGAKRVEPCKPEVANRHSGY
                     LIGGTSPFGTRKQMPVYVESSILEMDKIWLNGGRRGFLVSIEPGILTGLLAAKPVQCA
                     SVD"
     gene            2121..3683
                     /locus_tag="VHV99_17670"
     CDS             2121..3683
                     /locus_tag="VHV99_17670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX3637276.1"
                     /translation="MNLDSSTRSTIDIPALLAALPERIADIPALAAARDPQHVALIED
                     ARRLTNAQLLEAVDATAALLREWGVRGGDRVMIVAENSIVQIVLLFATAKLDAWALVS
                     NARLSAAELDAIRTHAQPRVVAYAVESSADAQQHAERHQAVTGPAITPDIGAWSYTLD
                     PTAKAEPVEAANHRQCAALIYTTGTTGAPKGVMLSHRNLLFIAAVSSRLRKVGPDDVV
                     YAVLPISHVYGFASVCLGSLHAGATLRLAPRFAPEAVRRALGEERVSILQGVPAMHAK
                     LLAHLQTHGHAWSAPHLRFAYSGGSPLDAALKAQVENVYGLPLHNGYGMTESSPTVSQ
                     TMLDAPRSDCSVGEVIPGVDVRFVGLDGVDAAPGEIGELWVRGPNVMLGYYRSPEQTR
                     AAVTEDGWLKTGDLARQDSDGALHIVGRSKELIIRSGFNVYPAEVEHVLNAHPQVVQS
                     AVIGRAVEGNEEVVAFVELIAGAKVSPAELIAWCAERLAPYKRPAEVRVLAALPAAST
                     GKILKHRLREFV"
     gene            complement(3788..4723)
                     /gene="xerD"
                     /locus_tag="VHV99_17675"
     CDS             complement(3788..4723)
                     /gene="xerD"
                     /locus_tag="VHV99_17675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEX3637277.1"
                     /translation="MSDTLTEDTPIASPLFAASSASIDAFCDALWLEHGLSRNTLDAY
                     RRDLRLFCEWLVHTRNTSLDTASEADLNAYSAARQKDKSTSANRRLSVFRRYYGWAVR
                     EHRAKVDPTVRIRSARQPPRFPSTLSEAQVEALLGAPDIDTPLGLRDRTMLELMYASG
                     LRVTELVTLKTVEVGLNDGVVRVMGKGSKERLIPFGEEAHGWIERYLREARPALLGAR
                     ATDALFVTSRAEGMTRQQFWNIIKRHAAAADVHAPLSPHTLRHAFATHLLNHGADLRV
                     VQLLLGHTDISTTQIYTHVARERLKSLHAQHHPRG"
     gene            complement(4720..5190)
                     /locus_tag="VHV99_17680"
     CDS             complement(4720..5190)
                     /locus_tag="VHV99_17680"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX3637278.1"
                     /translation="MFNAVIDAPFGKVGIRLEGEAVREIVYLPESTQNVAPDTPLAKQ
                     AVEQIERYFERASAKFELPLAAVGTAFQQRVWQAISDIPPGVVRTYGQLARQLGSAPR
                     AVGQACGANYFPIVIPCHRVVSSSGIGGFAHHADDGFFRNVKRWLLAHEGIPYA"
     gene            complement(5333..6574)
                     /gene="queG"
                     /locus_tag="VHV99_17685"
     CDS             complement(5333..6574)
                     /gene="queG"
                     /locus_tag="VHV99_17685"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEX3637279.1"
                     /translation="MNRSPESPVPCTPASNDDARAERHFDEAALNALALNIKTWGREL
                     GFGAIGISDTDLSAAEAPLAAWLEAGCHGEMDYMAKHGMKRARPAELVAGTRRVITAR
                     IAYLPAQTLSGKAHESALQDSPLAPQDWRAAEHARLADPSAAVVSVYARGRDYHKVMR
                     NRLQHLAEKIEAEIGAFGYRVFTDSAPVLEVELAQKAGIGWRGKHTLLLQRDAGSLFF
                     LGEIYVDVPLPTDAETSPEDAPETPGSHCGSCTRCIGACPTGAIVAPYKVDARLCISY
                     LTIELKGNIPLEMRPLIGNRVYGCDDCQLVCPWNKFAQAAPVDDFDVRHGLDRATLAE
                     LFAWSADEFDTRMQGSAIRRIGYESWLRNLAVVMGNALRDSSGSLSADAREAIVDALK
                     LRVEHPSAVVREHVEWALEAA"
     gene            6592..7212
                     /gene="tsaE"
                     /locus_tag="VHV99_17690"
     CDS             6592..7212
                     /gene="tsaE"
                     /locus_tag="VHV99_17690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX3637280.1"
                     /translation="MPVNPDLAIQPPANVLLERSFALADEAATNAFGERFAQAIESVR
                     ALPHEPRHNPQPATQQETQQHTPLAADEQTFHGLQVQLVGDLGAGKTTLVRATLRGLG
                     HIGRVRSPTYTLVEPYLLERPAGELALYHFDLYRFTDPAEWADAGFREYFDSGAICLV
                     EWPQRAGRLLGVPDLVFSLDLDHDGNGRVLVARAYSESGKACLERC"
     gene            7194..8786
                     /locus_tag="VHV99_17695"
     CDS             7194..8786
                     /locus_tag="VHV99_17695"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX3637281.1"
                     /translation="MSRKMLIKPFRSIESAATATHNWRRRQILRAGASTLMLGLVAPR
                     LAWASSVLGVRVWPARDYTRVTIESDQPLQNAQQLLQGPDRLVVDLSGLDLDQALKDL
                     VSQITPNDPQIQSVRVGQYQPHVVRMVFDLKGSVKPQVFTLPPVGAYKYRLVFDLYPA
                     VAPDPLMDLLAQTEHKQQTLDENNAPPATLSGPGTTPPTADNSEAFFERYAQNGGGGG
                     GGTAPGVPRPSVHGAPPVPPILGKPATPSAPVAPTAPPTAIARSKGGTSGNNLGGDDS
                     DSDDNYAFTNPKTGKSNTVRLLTVAIDPGHGGEDPGAIGGSGTYEKHVALDIAKKLRA
                     KIDGQPNMRAMMTRDADFFVPLNVRVQKARRVGADLFVSIHADAFTTPDAKGSSVFAL
                     SDHGASSAAARWMANKENSSDQIGGINIKSADATVNRALFDMSTTAQIRDSMRYGNFV
                     LKEIGGINKLHKGSVEQAGFAVLKAPDIPSILVETAFISNPDEERRLNDDAYREKMAD
                     AIMTGIKRYFAANPPLAKSRMT"
     gene            complement(8900..>9189)
                     /locus_tag="VHV99_17700"
     CDS             complement(8900..>9189)
                     /locus_tag="VHV99_17700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX3637282.1"
                     /translation="ALSGISAMSIFAIVYLAFIATLIGYGLWSRLLSRYPASQVAPFS
                     LLVPIVGLASASLFLDEQLSVAQIAGALLVMAGLAVNVFGGWVVQRFVAAR"
BASE COUNT         1786 a         3030 c         2824 g         1549 t
ORIGIN      
        1 cgacttcaag ctcggccagg tcaaggacgt cctgctgtcg aaaatggcca agcgcaatgt
       61 ggacgtgcgc tttctcgact acggcaagat cgagaagatc ggcggcgaca aggtcaagca
      121 gatcatcaag atcaagaaag gcgtgtccgg cgatctgtcg aagaaaatcg tgcgcctcgt
      181 gaaggacagc aagatcaaag tccaggcgag tattcagggc gacgcggtgc gcatcacggg
      241 cggcaaacgc gacgatttgc aaagcgtgat cgcgatgctg cgcaaggacg tgaccgatac
      301 cccgcttgac ttcaacaact tccgtgactg aacgcgcgtc atacagagta ccgggcgggc
      361 gtcaggcgcg gaaatgggtg ttgttgaggt tcggcgtata ttgaacgccc gccaccagcc
      421 gcatcaaagg ccgccacgga acgcctggct gcgttgagcc tcggcaccgg tgcgtgttga
      481 acgctcggca accagccgca tcaaggctcg ccacggagcg cctggctctg ttgagcctcg
      541 gcacctgtcc atgttgaacg ctcggcaacg aaccacgcag agattcagca ccgggcgctc
      601 acctgagttg aggcgcagca cccggcgtgt gccgaacgct caccacacag gcagtgcgct
      661 ttcagtgcgc actccgtaag ccaaccaggg ctcgccggct aacacccggt cggatgcgcc
      721 ctgctcgcgt tcaaggttcg ttaccgcctg ccgcgctagc cgctgcgtcg gttttcttct
      781 tggcgccgat gcggctttcc tggccatgca tcagcttggc gatgttgccg cggtggcgcc
      841 acaccagcag cgaactcatc acgacaatcg ccagcgcgat gatatgcggc ccgaacagaa
      901 aaccgtcgaa gatcggcgcg aacaccgccg cggccaatgc cgccagcgaa gaatagcgtg
      961 tgaagaatgc cacgatcagc cacgtcagca aggtggcgac gccgagaatc gggttgatcg
     1021 cgatcagcac gcctgctgcg gtggccacgc ccttgccacc cttgaagcgg aagaagatcg
     1081 gatacaggtg gccgagaaac acggcgacgg acgcaatcgc gaccgacgta tcgtcaagac
     1141 cgtagcgggt gccgaagtgc accacgaacc acaccggcag ccatcctttg aatgcatcgc
     1201 cgatcagggt gagaatcgcg gccttcttgc tgccgctgcg cagcacgttg gtggcgcccg
     1261 ggttgcctga gccgtacgag cgcgggtcgt ccagccccat cgcggcgctc acgatcacgg
     1321 caaacgacac cgagccgatc aggtaggcaa cgacagcaac aatcaggttt tgcatctggg
     1381 actcttataa ggatcggcgg tctgaaactc gatactccaa ccgcaacagg gaacaaagcg
     1441 gcgcacattc taccgaaccc acctgcccgg aaaacattca ggcaaacatc aatcgacgct
     1501 cgcgcactga accggtttcg cggccagcaa gccggtcaat atcccaggct cgatgctgac
     1561 cagaaaaccg cgccgcccgc cattcagcca gatcttgtcc atctcgagaa tgctcgactc
     1621 gacgtacacc ggcatctgct tgcgcgtgcc gaacggcgag gtgccgccga tcagatagcc
     1681 cgaatgccgg ttcgccactt cgggcttgca cggttcgacg cgtttggcgc caatctgccg
     1741 cgccagattc ttggtgctga cggtgcggtc gccgtgcatc agcacgatca gcggcttggc
     1801 atgctcgtct tccatcacca gggtcttcac gacatgatgt tcgtccacgc cgagctggcg
     1861 cgccgattcc tcggtgccgc cgtgctcgac gtaatcgtaa ggatgttcgc cgaaagtgac
     1921 accatggcgg cgcagaaact gcgtcgcggg cgtttcggag acgtgtctgg atttgctcat
     1981 cggcgcattg taatggcgag tttgcgcaac ggctatttgc cgaacggcca attgtgcggg
     2041 tctggcgatt gtggcacgat cgttcgtaaa tcctccggcg cacggcacgc cgggcatcga
     2101 atcgttttct ggagacagcg ttgaaccttg attcatccac ccgttccacg atcgacattc
     2161 ccgctctgct ggccgccctg cccgagcgta ttgccgacat tccggcgctc gccgccgcgc
     2221 gcgatccgca gcacgtcgcg ttgatcgagg atgcgcgccg cctcaccaat gcgcaacttc
     2281 tggaagccgt cgatgccacc gccgcgctat tgcgcgaatg gggcgtgcgt ggcggcgatc
     2341 gcgtgatgat cgtagccgag aacagcatcg tgcaaatcgt gctgctgttc gcgaccgcga
     2401 agctcgacgc gtgggcgctg gtgtcgaacg cacggctgtc cgcggccgaa ctcgacgcga
     2461 tccgtacgca cgcgcagcct cgcgtggtcg cctatgcggt cgaaagctcc gcggacgccc
     2521 aacagcatgc ggagcggcat caggccgtca cagggcctgc catcacaccg gatatcggtg
     2581 cctggtcgta cacgctcgac cccaccgcga aagccgaacc ggtcgaagcc gccaatcacc
     2641 gccaatgcgc cgcgctgatc tacaccacgg gaaccaccgg cgcgccgaaa ggcgtgatgc
     2701 tgtcgcatcg caatctgctc tttatcgcgg ccgtctcgag tcgcttgcgc aaggttgggc
     2761 cggacgatgt cgtgtacgcc gttctgccga tctcacacgt gtatggcttc gcctcggttt
     2821 gtctcggcag cttgcacgcc ggcgcgaccc tgcgcctcgc accgcgtttc gcgccggaag
     2881 ccgtgcggcg ggcgctcggc gaagagcggg tgtcgatctt gcagggtgtt ccggccatgc
     2941 acgccaaact gctcgcacat ttgcagacgc acggccacgc gtggtcggcg ccacatctgc
     3001 gctttgccta ttcgggcggc tcgccgctcg acgccgcgct gaaagcgcag gtcgaaaacg
     3061 tctacggcct cccgctgcac aatggttacg gtatgacgga gagcagcccg actgtctccc
     3121 aaaccatgct cgatgcgccg cgcagcgatt gctcagtcgg cgaagtcatt ccgggcgttg
     3181 acgtgcggtt cgtcggactc gacggcgtgg atgccgcgcc tggcgaaatc ggcgaactat
     3241 gggtgcgtgg cccgaacgtg atgctgggtt actaccgcag cccggagcag acccgcgccg
     3301 ccgtcaccga agacggctgg ctcaagaccg gcgatctcgc acggcaggac tcggacggtg
     3361 cattgcatat cgtcgggcgc agcaaggagt tgatcatccg ttcgggcttc aacgtgtacc
     3421 cggcggaagt cgagcacgtg ttgaacgcgc atccgcaagt ggtgcaatca gcggtgatcg
     3481 ggcgtgcagt ggagggtaac gaggaagtgg ttgcgttcgt cgagctgatc gcgggcgcga
     3541 aggtgtcgcc agctgaactc atcgcgtggt gcgcggagcg actcgcgccg tacaagcggc
     3601 ctgccgaagt gagggtgctc gccgcgttgc cggcggcgtc gactggcaag attctcaagc
     3661 accggttgcg cgagttcgtc taagcggcaa tggcttcggg tcggggtcgg ccctaagctt
     3721 tggccgagct gcgcaaagct accggcatac gcactcgccc ccacgcgaaa gcccacgcca
     3781 accctaatca cccccgcgga tgatgctgcg catgcagcga cttcaaccgc tcgcgggcca
     3841 cgtgcgtata gatttgcgtg gtcgagatat ccgtatgacc gagcagcaat tgcacgacgc
     3901 gcaaatcggc gccatggttc agcaggtgcg tcgcgaacgc gtgccgcagc gtatgtggcg
     3961 agagtggcgc atgaacatcg gccgccgccg cgtgacgctt gatgatgttc cagaactgct
     4021 ggcgggtcat gccctccgcg cggctcgtga cgaacagcgc atccgttgcg cgcgccccca
     4081 gcagcgcagg acgcgcttcg cgcagatagc gttcgatcca gccgtgcgcc tcttcgccga
     4141 acggaatcag gcgctctttg gagcccttgc ccatcacgcg caccacgccg tcgttcaggc
     4201 ccacttccac cgtcttcagc gtgacgagtt cagtgacacg caaaccgctc gcgtacatca
     4261 actccagcat ggtgcggtcg cgcagaccca agggtgtgtc gatatccggc gcaccgagca
     4321 gtgcttcgac ttgcgcttcg ctgagcgtcg acggaaaacg cggcggctgc ctggccgaac
     4381 ggatgcgcac cgtcggatcg accttcgcgc gatgctcgcg caccgcccag ccgtagtagc
     4441 ggcgaaacac cgagagccgc cggttcgccg aggtggattt gtctttctga cgcgccgcgc
     4501 tgtaggcgtt gagatcggcc tcgctagcgg tgtcgagcga cgtgttgcgg gtgtgcacga
     4561 gccattcgca gaacagccgc agatcgcgcc ggtacgcatc gagcgtgttg cgcgacaagc
     4621 cgtgctcgag ccacaacgcg tcgcagaacg cgtcgatcga ggccgaactg gccgcaaaca
     4681 aaggcgacgc gatgggcgtg tcttcagtca gggtatcgct catgcgtagg gaatgccttc
     4741 atgcgccagc agccagcgct tgacgttgcg aaaaaagcca tcgtcggcgt gatgcgcgaa
     4801 tccgccaatg ccgctcgaac tgaccacccg atggcacgga atcacgatcg gaaagtaatt
     4861 ggcgccgcac gcctggccaa ccgcgcgcgg ggcgctgccg agttgcctcg ccagttggcc
     4921 ataggtccgt acgacgcccg gcggaatgtc gctaatcgct tgccagacgc gctgctgaaa
     4981 cgcggtgccg accgccgcca gcggcaattc gaacttcgcg gaggcacgct cgaaataacg
     5041 ctcgatctgt tcgacggcct gcttcgcgag aggcgtgtcc ggcgcgacat tctgcgtcga
     5101 ctccggcaga tagacgatct cgcgcacagc ctcgccttcg aggcggatgc cgaccttgcc
     5161 gaacggtgca tcaatcactg cgttaaacat ggtacttgct ccgtgatagg cggggcgctc
     5221 ctgtatttgc acgacggcga aattcaatcg ccgcatcaat aaccgcgggc agcgtgcaac
     5281 gcgccgtcac ccgccgcggg ccctgccacg cccccgcccg gttgccgcta ctttacgccg
     5341 cttccaacgc ccattccaca tgctcgcgca ccacggcgga agggtgttca acacgtaact
     5401 tcagcgcgtc aacaatcgcc tcgcgcgcgt ccgcgctcaa actcccagaa gaatcacgca
     5461 gcgcattacc catcaccacc gccaggttac gcagccagct ttcatagcca atgcggcgaa
     5521 tcgcactccc ctgcatgcgc gtatcgaatt cgtcggcgct ccacgcaaag agttcggcga
     5581 gcgtcgcgcg atcgaggcca tgccgcacat cgaaatcgtc gaccggcgcg gcctgcgcaa
     5641 acttgttcca cggacacacg agctggcaat cgtcgcagcc gtacacacga ttaccgatca
     5701 gcggccgcat ttccagggga atattgccct tcaattcgat cgtcagatac gaaatgcaca
     5761 gacgtgcatc gaccttataa ggcgccacga tcgcacccgt cgggcacgcg ccgatgcagc
     5821 gcgtgcaact gccgcagtgc gagcccggcg tttcgggcgc atcttccggc gacgtctcgg
     5881 catcggtggg caacggcaca tcgacataga tttcgccgag gaaaaacagc gaaccggcat
     5941 cgcgttgcag cagcagcgtg tgcttgccac gccagccgat gccggccttc tgggcaagtt
     6001 ctacctcgag caccggcgcc gaatcggtaa atacgcggta accgaatgca ccgatctccg
     6061 cctcgatttt ctcggccaga tgttgcaaac ggttacgcat gaccttgtga taatcgcggc
     6121 ctcgcgcata gaccgacacc accgccgccg acgggtccgc gagccgcgca tgctcggctg
     6181 cgcgccagtc ttgcggtgcg aggggcgagt cctgcaacgc gctttcgtgc gcctttccac
     6241 tcaatgtctg cgcgggcaaa taggcgatgc gcgcggtaat cacacgtcgc gtgccggcca
     6301 caagctcggc ggggcgcgcg cgtttcatac cgtgtttcgc catataatcc atctcgccgt
     6361 ggcaacccgc ttccagccat gctgcaagcg gcgcctcggc agcggagaga tcggtgtcgc
     6421 taatgccgat cgctccgaaa cccagttcac ggccccacgt tttgatgttg agcgcgagcg
     6481 cattcaacgc cgcttcatcg aaatgacgct cggcacgcgc atcgtcgtta gacgcaggcg
     6541 tgcagggaac aggggactcc ggacttcggt tcatcacgcc attttacgag aatgcccgtc
     6601 aatcccgatc tcgcgatcca accgcccgcc aatgtcctgc tcgaacgctc cttcgcgctc
     6661 gcggacgagg ccgccacgaa tgcattcggc gagcgcttcg cacaggcgat cgagagcgtg
     6721 cgcgcgttgc cgcacgagcc acgacacaac ccgcagccgg caacacagca ggaaacgcaa
     6781 caacacaccc cgctcgccgc ggacgagcaa acattccacg gactgcaagt gcaactcgtc
     6841 ggcgacctcg gcgccggcaa aaccaccctc gtgcgcgcca ccctgcgcgg cctcggccac
     6901 atcggccgcg tgcgcagtcc tacttacaca ctcgtcgaac cctacctgct cgaacggccc
     6961 gctggggaac tcgcgctcta tcacttcgat ctgtacagat tcaccgatcc ggccgaatgg
     7021 gctgacgcgg gctttcgcga gtacttcgac agcggcgcca tctgcctcgt cgaatggccg
     7081 cagcgcgcgg gccgcctgct gggcgtgccg gatctcgtct tctcgctcga cctggaccac
     7141 gatggcaacg gccgcgtact cgtcgcacgg gcatatagcg aatcaggaaa ggcatgtctc
     7201 gaaagatgtt gatcaaacca ttccgctcga tcgaatcggc ggcgaccgca acgcataact
     7261 ggcggcgccg gcagattctg cgcgcgggcg cgtccacgct gatgctcggc cttgtcgctc
     7321 cgcgtctcgc gtgggcgagt tcggtgctcg gcgtgcgagt ctggccggca cgcgactaca
     7381 cgcgcgtgac gatcgagtcg gatcagccat tgcagaacgc acagcagttg ctgcagggtc
     7441 ctgaccggct ggtggtcgac ctgagcggcc tcgatctgga tcaggcgtta aaggatctgg
     7501 tgtcgcagat cacgccgaac gatccgcaga tccagtcggt gcgcgttggg caatatcagc
     7561 cgcacgtcgt gcgcatggtg ttcgatctga agggttcggt gaagccgcaa gtttttacgc
     7621 tgccgccggt cggcgcgtac aagtaccggc tggtgttcga cctgtatccc gccgttgcgc
     7681 ctgacccgct aatggatctg ctcgcgcaga ccgagcacaa gcagcaaacg ctggacgaaa
     7741 acaacgcgcc gcccgccacg ttaagtggcc ctggcactac gccgcccacc gccgacaaca
     7801 gcgaagcttt cttcgagcgt tacgcgcaga acggcggcgg cggcggcggt gggaccgcgc
     7861 cgggcgtgcc gcgcccgtcc gtgcatggcg cacctccggt tccgcccatc ctcggcaagc
     7921 ctgcaacccc gagcgcgccg gttgcgccca ccgcgccgcc caccgcaatc gcccgcagca
     7981 agggaggcac cagcggcaac aacctgggtg gcgacgacag cgacagcgac gacaactacg
     8041 ccttcaccaa cccgaaaacc ggcaagagca acacggtgcg tctgttgacc gtcgcgatcg
     8101 atccgggtca cggcggcgaa gacccgggcg cgatcggcgg ctcgggcacg tacgagaagc
     8161 acgtcgcact cgacatcgcg aagaagctgc gcgccaagat cgacggccag ccgaacatgc
     8221 gcgccatgat gacccgcgac gccgacttct tcgtgccgct gaacgtgcgc gtgcagaagg
     8281 cgcggcgcgt gggcgcggac ctgttcgtct cgatccacgc ggatgcgttc accacgcccg
     8341 atgccaaggg ctcgtcggtg ttcgcgttat cggatcatgg cgcctcgagc gccgcggcgc
     8401 gctggatggc gaacaaggag aactcgtcgg atcagatcgg cgggatcaac atcaagtccg
     8461 ccgacgccac cgtcaaccgc gcgctgttcg atatgtcgac caccgcgcag atccgcgatt
     8521 cgatgcgcta cggcaacttc gtgctgaagg aaatcggcgg catcaacaag ctgcacaaag
     8581 gttcggtgga acaggcgggg tttgccgtgc tgaaggcgcc ggatattccg tcgatcctcg
     8641 tggaaacggc cttcatcagc aacccggacg aggaacgccg cctgaacgac gacgcgtatc
     8701 gggagaaaat ggccgacgcg atcatgaccg gcatcaagcg ctatttcgcg gcgaatccgc
     8761 cgttggcgaa gagccgcatg acgtaatgtc atcgggcacg gcagtgggtc atgcgaagcc
     8821 ctgatggcga atagaagatc ggccgctgat gcggccgttt tctttgccca cgcgagtcga
     8881 tctacgcgac gacgcgcgct cagcgcgccg ccacaaatcg ctggacgacc cacccaccga
     8941 acacattcac tgccaatccc gccatcacga gcagggcgcc cgcgatctgc gccacggaca
     9001 actgctcgtc gagaaacagg gacgccgaag ccagcccgac aatcggcacc agcagcgaga
     9061 acggcgccac ctggctcgcc gggtagcgcg acaacagccg gctccacaag ccatagccga
     9121 tcagcgtggc gataaacgcg aggtagacga tggcgaaaat cgacattgca ctgatgccgg
     9181 acagcgctg
//
LOCUS       DATASH010000656         4123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5647972,
            whole genome shotgun sequence.
ACCESSION   DATASH010000656 DATASH010000000
VERSION     DATASH010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5647972"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            519..764
                     /locus_tag="VHV99_17705"
     CDS             519..764
                     /locus_tag="VHV99_17705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637283.1"
                     /translation="MRKLLLSAVLIASIATPALAQSYNPEFGTGNVENMPLAGQSNDG
                     ANSAYAHEPAQTQSNVSPRHIRRRSSAAKKHYQIAPN"
     gene            complement(1579..2508)
                     /locus_tag="VHV99_17710"
     CDS             complement(1579..2508)
                     /locus_tag="VHV99_17710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637284.1"
                     /translation="MFNRKLALAGILLAATAGLANAADLPTKTPVLKAPADVPFFFVN
                     DNSISYHYEFTATNPGAGQTPKNVLSFTHFDVWKYGTNFFNVDWLKATSSSTPTASGT
                     QGYTEIYGFFRSTFGFNEIFNTKAFAVGPLTDVSLMVGADLNTDNTGLNSAKRSIEAG
                     IQFSFLAPYRGFINLSPTVYKEWQHDGYAAMAGTNPSGAVDFDTTWGFEWLYVQPLGF
                     LPPSIPLTYKFFGTIHGAKGSGEGTAEPDRTTEYYLQQNLSLDVGQMLGQRPNMVSLW
                     GGYRYWYNKFGINHNDMPYSIESTWLTGVTVAF"
     gene            3072..>4123
                     /locus_tag="VHV99_17715"
     CDS             3072..>4123
                     /locus_tag="VHV99_17715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3637285.1"
                     /translation="MNVSKVAALTMLMPANSPSDEGPLLQAIGITKRYGAFLANDRID
                     LDLLPRDIHALLGENGAGKSTLVKIIYGLIQPSAGELRWMGKTVEFAGPSEARALGIG
                     MVFQHFSLFDNLTVAENVALGLDGSESFAAMSARLAQVSRDYGLPLDPKREVWRLSVG
                     ERQRIEIVRMLMQNPKLLILDEPTAVLTPQEADQLFIVLERLKGEGRSILYISHKLDE
                     VKRLCDTATILRGGKKVATCDPRRETAASLARMMVGAEIGEVKAPSGRKTTVPRLVVQ
                     DLSLAPDDPHGVHLKAVSLEVKGGEIFGIAGVAGNGQDELFAALSGERRASREGNVII
                     DGNGAGTLSVTERRRLG"
BASE COUNT          869 a         1208 c         1212 g          834 t
ORIGIN      
        1 gggcgctatg cagcgctacc gttttcgaag aggcatgaag cgtttcgggt ggggactgaa
       61 catcgtctgt atactgatta ttatttccgc gacaagagat gcttcgcgcg cgccaacact
      121 ttggacccaa tagacttgcc gcttcgaaag attgtggttc accacgttgt gactacgacg
      181 tcatgaagca aaaatgagaa tcgacgcgcg acttttgcga tgacgtctcg aatgctcgcg
      241 gttgccaaaa acggcggcgt aatgtcgcag cccaacgact ccttctcttc actgtgatcg
      301 aatttcatcg ttatggacga agggcgcgcg gagtagtctt cgcctttcgt tgcaagactg
      361 caaggctgac gatgttgttc aagccgatac gcacatagca atttcgagca agcggactca
      421 gcctgacgat cctatcgctg tgtctatagc ggcggagggc cgctgtgctt ggcgctccct
      481 ggggcgccaa cagtagataa ttaagaagga cacacgcagt gaggaaactt ctgttgagcg
      541 cggtcttgat cgcgtcaatc gcaacacctg ctctggccca atcgtacaac ccggagttcg
      601 gcacaggcaa tgtcgagaac atgccgctag ccggacaaag caacgacggc gcgaacagtg
      661 cctacgccca tgagcccgct cagacgcagt ccaatgtaag cccgcgccac atccggcgcc
      721 ggagttctgc ggccaaaaag cactatcaga tcgcgccgaa ctaggacgaa aacgactcgt
      781 cacgcgcggc ctccgttcgt cgggacaaca agtgccgtac acctgaaagt ctagtgccct
      841 agcttacgtg tccggctgtg cgtttccttc agcgacgcga tgatgccgtg taatagactt
      901 tcgttgcccc gccgggtggc cgtccgtggt tgtcggacgc gcaaggtcca ttgcgctacg
      961 cctcaataaa acaacgcgcc agtcgattgc tcgaccggcg cgttacccat ctgctgcgat
     1021 tgagtcgttg gccgattgtc cgcgcacacc gtcacacgtg gcaggacgcg aggctggcaa
     1081 ttgcgagcaa gaaaacagca atttcgagca agcaagttca gcccgaagat cctacggcta
     1141 ttttcgtgac ggcggatggt cgctgtgctt ggcggaaaac tgcggaagac ccaaccatga
     1201 atagatattc aggacactcg cagatgaagc cacgtctgtt gcgcgcggtc ttgatcgcgt
     1261 caaatcgcga cacccactct ggcgcaattg tacgacccgc acgtcggcac agccaaagtc
     1321 aaggacatgc ggcaggccga acgccgcgac ggcggcgcga agtgcctgca ctcacgcgtt
     1381 cacgacgcca cccacgcaag tcacgtgccc tggtggtttt accctggagc tcttcgacct
     1441 gccgcatctg ttaactcaac gatctcggct gagctcgcgt cccaaaacgc tttcccaaaa
     1501 gcaacgcgcc ggtcgattgc tcaaccggcg cgccgttcat gtgcctggtg ccccccagcc
     1561 tcaggcagtg gcttcgtctc agaaggcgac cgtcacgccg gtgagccagg tgctctcgat
     1621 cgaatacggc atatcgttat ggttgatgcc gaacttgttg taccagtagc gatagccacc
     1681 ccagagcgaa accatgttcg ggcgctggcc gagcatctgg ccgacgtcga ggctcaagtt
     1741 ctgctggaga tagtattcgg tcgtacgatc gggctcggcg gtgccttcac ccgagccctt
     1801 cgcgccatgg atcgtgccga agaacttata ggtcagcggg atcgacggcg gcaggaagcc
     1861 gagcggctgc acatacaacc attcgaagcc ccacgtcgtg tcgaaatcca cagcgcccga
     1921 cgggttggtg cccgccatcg ctgcgtagcc gtcgtgctgc cattccttgt agacggtcgg
     1981 cgacaagtta atgaagccgc ggtagggcgc caggaagcta aactggatgc cggcttcgat
     2041 cgaccgcttg gcggaattca gccccgtgtt gtcggtgttg aggtccgcgc cgaccatcag
     2101 cgagacgtcg gtcagcggac cgaccgcgaa ggccttcgtg ttgaaaattt cattgaagcc
     2161 gaaggtactg cggaagaagc cgtagatctc agtgtagcct tgcgtgcccg acgcagtcgg
     2221 cgtcgaagag ctggtggcct tcagccaatc gacattgaag aagttcgtgc cgtacttcca
     2281 cacgtcgaag tgcgtgaagg agagcacgtt ctttggtgtc tggccggcgc ccggatttgt
     2341 cgccgtgaat tcatagtgat agctgatcga attgtcgttg acgaagaaga acggcacatc
     2401 ggcaggagcc ttcaacaccg gcgtcttggt aggaagatcg gccgcgttcg cgagaccggc
     2461 cgtcgccgcc agaaggattc cggcgagcgc taattttcga ttgaacattg ttggaccccc
     2521 aacgagttgt gacgaagcga aactgtcaca aatgctatgc ggtgctattc ttttggggga
     2581 agcctgaaga ttgcgcgtgt tctgatgatt atttcatcca gatcaaacaa acgcggcgcc
     2641 ggccttcaat tcacgtattt tatgcggata atatgaatcg ccttatcgta actaccgatt
     2701 ttacacattt tcgataatgc ggcggccaaa ctctgcgagg ccgccgccat tcattgttcg
     2761 tgactaaagc aaaggcacga aaggtcgcag gctaccgatt gttttccact gtgatggaat
     2821 tgcaacgctc gctgatgcgg cgaaagcaca tgacctcttc gtctttcgtc gtgcatcttc
     2881 gcgaaccgga agccacggcg gtgccccctc gccggctaaa tcggacggac gaatcgtaac
     2941 ggcccaaggg agcccgcgga cgtttacggc tgcctccatt gcagcagttg acgaggtttt
     3001 gggcagtcat tgccactatt tcgtggcttc taatgccgcg aattttggca cattattaaa
     3061 ctgatgcttt gttgaacgtc tcgaaggtcg ccgctctaac catgttgatg cctgcgaata
     3121 gcccgtctga tgaaggccca ctgcttcagg ccatcggcat caccaaacga tacggagctt
     3181 tcctcgcgaa cgaccggatc gatctcgacc ttctgccgcg cgatatccac gcgctgctcg
     3241 gtgagaacgg cgcgggcaaa tccacgctgg tgaagatcat ctacggcctc atccagccat
     3301 ccgcgggcga actgcgctgg atggggaaaa cggtggagtt cgccggccca tccgaggcgc
     3361 gcgcgctagg catcggcatg gtgttccagc atttctcgct gttcgataac ctgactgtcg
     3421 cagagaatgt cgcgctgggt ctcgatggca gcgaaagttt cgcggccatg tcggcgcggc
     3481 tggcgcaagt gtcgcgcgac tacggtctgc cgctcgatcc caagcgcgag gtgtggcggc
     3541 tctccgtcgg ggaacgccag cgcatcgaga tcgtgcgcat gttgatgcag aacccgaagc
     3601 tcttgatcct cgacgagccg acagcggtgt tgacgccgca ggaggccgac caattattta
     3661 tcgtgctcga gcgtcttaaa ggtgaaggcc ggtccatcct ctacatcagc cacaaattgg
     3721 atgaggtgaa gcggctgtgc gacacggcca cgattttgcg cgggggcaag aaggtcgcca
     3781 cctgcgaccc gcggcgcgag accgccgcgt cattggcgag aatgatggtc ggcgccgaga
     3841 tcggcgaggt aaaggcgccg agcggacgca agacaaccgt gccgcggctc gtggtgcagg
     3901 atctctcgct cgcacccgac gatccgcacg gcgtccatct caaggccgtc tcgctcgagg
     3961 tcaaaggcgg agaaatcttc ggcatcgccg gcgtcgccgg caacggccag gacgaactgt
     4021 tcgccgcact ctccggcgaa cggcgcgcat cccgcgaagg caatgtgatc atcgacggca
     4081 acggcgccgg taccctctcc gtcaccgagc gccgccgcct cgg
//
LOCUS       DATASH010000657         4645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1889320,
            whole genome shotgun sequence.
ACCESSION   DATASH010000657 DATASH010000000
VERSION     DATASH010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1889320"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..1493
                     /locus_tag="VHV99_17720"
     CDS             99..1493
                     /locus_tag="VHV99_17720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX3637286.1"
                     /translation="MEPATRQLIYVSRDPSAELNTRFHQRGWHVEVVGSARDVRRAVR
                     AGMAAGGLLDLSSDFQPHEIAAFESCLTMPNVGWVAATMPGQLQDAALRRLVRDYCFD
                     YVTVPYSGDRIVDSVGHAYGMISLGEPASNDGSQGAEGEMVGSCDAMLALFRSIRKVA
                     MTDAPVFISGESGTGKELTAVAIHERSARRNAPFVPINCGAIPPHLLQSELFGYERGA
                     FTGANQRKIGRVEAANGGTLFLDEIGDLPLESQASLLRFLQERKVERLGGHGSIDVDV
                     RIISATHVDMTSAMIEGRFRSDLYHRLCVLQIDEPPLRARGKDIELLARHMLERFKKD
                     ASRRLRGFAPDAIAALHNYGWPGNVRELINRVRRAIVMSEGRAITARDLELAEYVEIV
                     PVSLAQAREAAERQAIELALLRHRGRLGDAAQELGISRVTLYRLLCSHGMRHMEGEPL
                     VTPHGDLPASVPHL"
     gene            1750..2721
                     /locus_tag="VHV99_17725"
     CDS             1750..2721
                     /locus_tag="VHV99_17725"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006400684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEX3637287.1"
                     /translation="MKQYLDLVRTILDTGTWQENRTGIRTISMPGAMLRFDLQQGFPA
                     VTTKKLAFKSAVGELVGFLRASRSAADFRDLGCKVWDANANQNPQWLANPYREGPDDL
                     GDVYGVQWRQWPAYKVLDAGASAQLADATARGFHVVTEFEEGGSAKVLLYKAIDQLRQ
                     CLDTIMQNPADRRILFHAWNPAVLDQIALPACHLLYQFLPNVARREISLCLYIRSNDV
                     GLGTPFNLTEGAALLHLVGRLTGYTPRWFTYFIGDAHIYENQLDMLQQQLTREPYESP
                     AFEISERVPDYAQTGVYEPEWLEKIEPSDFSLVGYRHHEPLTAPMAV"
     gene            complement(2846..>4645)
                     /locus_tag="VHV99_17730"
     CDS             complement(2846..>4645)
                     /locus_tag="VHV99_17730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HEX3637288.1"
                     /translation="PWGWTWVDDAPWGFAPYHYGRWAQVDDTWAWVPGPVVVDEPPAY
                     APALVAFVGGDGGGGVDWGVNLAIGGVVAAGVAWFPLGPGERWHPNWGGHNHWSPGYY
                     NRVNNYNRNVKNVTNIHNTYINYRVPGGVTAVPATAFVHGQPVGRFAQKVDPQQWRNA
                     RINPGGPGIAPVKESFGPGQRNANYRPPANVMARSVVATRSPAVPPAYHDSLAQRFAQ
                     NGGRVPGAGAPIVRTAVPARMAGGPGAMPVQNVRVVQSHLAGRTPGAAPGAAPGAPGM
                     PGMPGMPNGMQPAGQRPGEASVNGQPLRGGAPQARPGMPPQIANARPQPSEAVHPSNG
                     VPRPPQANGANPNAYAQPHGAPGQAGQQPAVEGRHEPAWTQPHTPMAQQAQQRGAPQE
                     QGRQFGVRPEPQQQAQRQPEAQPQRPPEVHASPQEPRPQPMQPMQPMQQAQQRQEYHP
                     QQQQAVQPRPEFRPQQVQQARPEPQQVQQVQQPRPAPGPQQVQQPRPEPGPQQVQQPR
                     PEPRPQQVQQPRHEPRPQQVQQPRSEPRPQQVQQPRPEPRQQQVQQPRPEPRPQQVQQ
                     PRPQPQHVEQPRPQPQHAEQHSGGGNRDEHHKG"
BASE COUNT          722 a         1490 c         1536 g          897 t
ORIGIN      
        1 ttttgctatt tcgcgaatcc cgaacgagtc agcgtgtctg aactccggtt caggtgcgtg
       61 cgtcatcctt aacgttcgtt gacgagagga tctgaataat ggaacccgca acgcggcaac
      121 tgatttacgt ttcgcgcgat ccgagcgcgg aattgaatac tcggtttcat cagcgcggct
      181 ggcatgtcga agtcgtggga tcggcgcgcg atgtgcgccg tgcggttcgc gccggaatgg
      241 cggcgggcgg cctgctcgat ctgtctagcg attttcagcc acacgaaatc gctgccttcg
      301 agtcctgcct gaccatgccg aacgtcggct gggtcgcggc caccatgccc ggacaattgc
      361 aggacgccgc cttgcgccgg ctcgtgcggg actattgttt cgactatgta acggtgcctt
      421 actcgggtga caggatcgtc gattcggtcg gtcatgcata cggcatgatc tcgctgggcg
      481 agccggcatc gaatgacgga tcgcaaggcg ccgaaggcga aatggtcggc tcgtgcgacg
      541 cgatgctcgc gctgttccgt tccatccgca aagtggcgat gaccgacgcg ccggtgttta
      601 tctcgggcga atcgggcacg ggcaaggaat tgacggcggt ggcgatccac gaacgctcgg
      661 cgcgccgcaa tgcgcctttc gtgccgatca actgcggtgc gattccgccg catctgctgc
      721 aatccgaact atttggctat gagcgcggcg cgttcaccgg cgccaatcaa cgcaagatcg
      781 gtcgcgtgga agcagcaaac ggcggcaccc tgtttctcga cgaaatcggc gatctgccgc
      841 tcgaaagcca ggcaagcctg ctgcgcttcc tgcaggaacg taaggtggag cggctgggcg
      901 gtcacggttc catcgacgtc gacgtgcgca tcatctcggc aacccacgtg gatatgacgt
      961 ccgcgatgat cgaagggcgg ttccgctccg acctgtatca ccgtctgtgc gtgctgcaaa
     1021 tcgacgaacc gccgttgcgg gcgcgtggta aggatatcga actgcttgcg cggcacatgc
     1081 tggaacgttt caagaaggat gcaagccgcc gtctgcgcgg tttcgcgccc gacgcgatcg
     1141 cggcgctgca taactacggc tggccgggca acgtgcgcga gttgatcaat cgcgtgcggc
     1201 gcgccatcgt gatgtcggag gggcgcgcga ttacggcgcg cgatctcgaa ttggccgaat
     1261 acgtggagat cgtgccggtg tcgctcgcgc aggcgcgcga agcggccgaa cgtcaggcaa
     1321 tcgaactcgc gcttctgcgt catcgcggcc gtctcggcga cgccgcgcag gagctcggta
     1381 tttcccgcgt gacgctatac cgtctgttgt gctcacacgg catgcgccat atggaaggcg
     1441 aaccgctggt cacgccgcac ggcgatttgc cggcctcggt gccgcatctc taggctgtat
     1501 ttgctgacct taccgacttc aggcccgaaa aggccggcag caactgccgg tgggcctgcc
     1561 gtgggcgctg acggcattga tgacgcggtc cgcgcaagcc gggtccagag cggcttgatc
     1621 ttcaatattt tctcttccgc gcgcccgggc cgggcgccac ctggccacgc ttgttaaaat
     1681 cgggttttcc gctcaatctc cgcggcgcct gctcctgacc caagggcgcg cttcgtcgaa
     1741 ggaacccgca tgaaacaata cctcgacctc gtccgcacga ttctcgacac cggcacgtgg
     1801 caggagaacc gcaccggcat ccgcaccatc agcatgccgg gcgccatgtt gcgcttcgac
     1861 ctccagcagg gttttcccgc agtcaccacc aaaaagctgg cgttcaaatc ggcggtgggg
     1921 gaactggtcg ggtttctgcg cgcctcgcgc agtgccgcgg atttccgcga ccttggctgc
     1981 aaggtgtggg acgcgaacgc caatcagaat ccgcagtggc tcgccaatcc gtatcgcgag
     2041 ggcccggacg atctcggcga cgtgtacggc gtgcagtggc gccagtggcc ggcctacaag
     2101 gtgctggacg ccggcgcaag cgcgcaactg gctgacgcca ccgcgcgcgg cttccatgtg
     2161 gtgacggagt tcgaggaagg cggcagcgcc aaggtcctgc tgtacaaggc gatcgaccag
     2221 ttgcggcaat gtctcgacac gatcatgcag aaccccgccg accggcgaat tttgtttcat
     2281 gcctggaacc ccgccgtgct ggaccagatc gcgctgccgg cttgtcacct gctttatcag
     2341 ttcctgccga acgtcgcgcg gcgtgaaatt tcgctgtgtc tgtacattcg cagcaacgac
     2401 gtcggcctcg gcacgccgtt caatctgacc gaaggcgcgg cgctgttgca tctggtcggc
     2461 cggctgacgg ggtatacgcc gcggtggttc acttacttca tcggcgacgc gcatatctac
     2521 gaaaatcagc tcgatatgct gcagcaacag ctcacgcgcg agccgtacga aagccccgcg
     2581 ttcgagatct cggagcgggt gccggactat gcgcaaactg gggtgtatga gccggaatgg
     2641 ctcgagaaaa tcgagccctc ggacttttcg ctggttggtt accggcatca tgagccgctc
     2701 acggcgccga tggcggtctg aacacggtct gagcgccgat tcgcagcctg ctcaaagaaa
     2761 aacccccgcg acgtgagtcg cgggggtttt ttcttgccgc tttccggcca tcaagttgtc
     2821 agcgcgttcg ctgcgcgcgt aaccgttagc ctttgtgatg ctcgtcacgg ttgccgccgc
     2881 ctgaatgctg ttcggcgtgc tgcggctggg gacgtggctg ctccacatgc tgcggttgag
     2941 ggcgcggctg ttgtacctgc tgcggacgcg gttcagggcg tggctgctgc acttgttgct
     3001 gacgtggttc agggcgcggc tgctgcactt gttgcggacg cggttcagag cgcggctgct
     3061 gcacttgttg cggacgcggt tcatggcgcg gctgctgcac ttgttgcgga cgtggttcag
     3121 ggcgcggctg ctgaacctgt tgcggacctg gttcagggcg cggctgctgt acctgctgtg
     3181 gaccaggtgc agggcggggc tgctgcacct gttgcacctg ctgcggctca gggcgagcct
     3241 gctggacctg ttgcggacgg aactcaggac gcggctgcac tgcctgttgc tgctgtggat
     3301 gatattcctg acgctgctgc gcctgctgca tgggttgcat tggctgcatc ggttgcggcc
     3361 ggggctcctg tggcgatgcg tgtacttcag gcggacgctg cggctgcgct tccggttgac
     3421 gctgagcctg ttgctgcggc tcaggacgaa ccccgaactg gcgcccctgt tcctgcggcg
     3481 cgccgcgctg ctgcgcctgt tgggccatcg gcgtgtgcgg ttgcgtccat gcgggctcgt
     3541 gccgaccttc cacggccggt tgctgacccg cttgccccgg cgcgccgtgc ggttgcgcgt
     3601 aagcgttcgg attcgcgccg ttagcttgcg gcggacgcgg cacgccattc gacggatgaa
     3661 ctgcctcgct gggttgcggg cgtgcattgg cgatttgcgg cggcatcccc ggtcgcgctt
     3721 gcggcgcgcc gccacgtagc ggttgaccgt tgaccgatgc ctcgcccggc cgctgacccg
     3781 ccggttgcat gccattcggc atacccggca tacccggcat accaggcgca cccggcgcgg
     3841 cacccggcgc ggcacccggc gtccgcccgg ccagatgcga ctggacgacc cgcacgttct
     3901 gcaccggcat tgctcccgga ccgcccgcca tgcgagccgg caccgcggtt cgcacgatcg
     3961 gcgcgcctgc gcccggcacc cgtccgccgt tctgcgcaaa gcgttgcgcg agactgtcgt
     4021 ggtacgcggg cggcacagca ggactgcggg tggcgacgac cgaacgcgcc atcacgttgg
     4081 ctggcgggcg gtagttcgcg ttacgctggc caggtccgaa gctttccttc accggcgcga
     4141 ttcccggacc gcccggattg atccgcgcgt tgcgccactg ctgcggatcg accttctggg
     4201 cgaagcggcc gaccggttga ccgtgcacga aggctgttgc cggcaccgcg gtcacgccgc
     4261 ccggcacgcg atagttgatg tacgtgttat gaatgttggt cacattcttc acgttgcggt
     4321 tgtagttgtt gacgcggttg tagtaaccgg ggctccagtg attgtgaccg ccccagttcg
     4381 gatgccaccg ctcgcccggg cccagcggga accacgcgac gccggccgcg acgacgccgc
     4441 cgatcgccag attcaccccc cagtcgactc cgccaccacc atctcccccc acgaaagcga
     4501 ccagcgccgg cgcgtaggca ggcggttcat cgaccacgac cggtcccggc acccacgccc
     4561 acgtgtcgtc gacctgggcc cagcggccgt agtggtaggg cgcgaaaccc cacggcgcat
     4621 cgtcgaccca ggtccagccc cacgg
//
LOCUS       DATASH010000658         1636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4461349,
            whole genome shotgun sequence.
ACCESSION   DATASH010000658 DATASH010000000
VERSION     DATASH010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4461349"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="VHV99_17735"
     CDS             complement(<1..908)
                     /locus_tag="VHV99_17735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HEX3637289.1"
                     /translation="MTTVIRSIDNGWRRWIAENLLLDAPPPAVHGALVGHGFDPAEAV
                     REIEAALASPCIEGAARLRNRLRKREWILSVYGTLNRMRAQSGAIERRERLSRDEFFE
                     QYYFQNRPVIITGAFDFWPARTQWCFDYFRERCGDCEVEVQFGRESDANYEINQPKLK
                     RMMRFADYVDLVERSNLTNDFYMTANNTSHNRAALAALWPDVPTIGEYLDAGSPDTGF
                     FWMGPAGTKTPFHHDLTNNFMAQIIGRKQIKMVPLSDTPQMANYLHCFSQVDGSAIDY
                     ERFPSMRDAHLIECTLAPGELLFLPIG"
     gene            complement(1049..>1636)
                     /locus_tag="VHV99_17740"
     CDS             complement(1049..>1636)
                     /locus_tag="VHV99_17740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX3637290.1"
                     /translation="NEFEKTAKSLGLNVMSHDATNDKAVDFRAILTKIKGENPDAIMY
                     GGMDATGGPFAKQAKQLGLRAKVLAGDGVCTDKLSDLAGDATDNIVCSEAGMALEKMA
                     GGPAFLAKYQKRFGQPIQIYAPFTYDAVYIIVDAMKRANSTDPAKILAAMPATDYKGV
                     IGETTFDSKGDLQHGVISLYNYKGGKKTLLDVVKM"
BASE COUNT          336 a          497 c          462 g          341 t
ORIGIN      
        1 ccgatcggca gaaacagcaa ttcaccgggt gcgagcgtgc attcgatcaa atgtgcatcg
       61 cgcatcgacg ggaagcgttc atagtcgatc gcactgccat ccacctgcga aaaacaatgc
      121 agatagttcg ccatctgagg cgtatcggac aagggcacca tcttgatctg cttgcggccg
      181 attatctgcg ccatgaagtt attggtcaga tcatggtgaa acggcgtctt cgttccagcc
      241 ggccccatcc agaaaaagcc ggtatcgggc gatccggcgt cgagatattc gccgatggtc
      301 ggcacatcgg gccataacgc ggcgagtgcc gcgcgattgt gcgaggtgtt attagccgtc
      361 atgtagaaat cgtttgtcag gttgctgcgc tcgacgagat cgacataatc ggcaaagcgc
      421 atcatccgtt tgagcttcgg ctgattgatt tcataattcg catccgactc gcggccgaac
      481 tgcacctcga cctcgcaatc gccgcaacgc tcgcgaaagt aatcgaaaca ccactgcgtg
      541 cgcgccggcc agaaatcgaa agcgccggtg atgatgaccg gccggttctg aaagtagtac
      601 tgctcaaaga actcgtcacg cgatagacgc tcgcgccttt caatcgcgcc gctttgcgcg
      661 cgcatgcgat tgagcgtgcc gtacaccgac aatatccact cgcgcttgcg caaacgattg
      721 cgcaagcgcg ctgcaccctc aatgcaagga ctcgcgagcg ctgcctcgat ttcgcgcacg
      781 gcctcggcag ggtcgaaacc atgtccgacc agcgcaccat gcactgcggg cggcggtgcg
      841 tcgagcagta gattctcggc aatccagcgg cgccagccgt tatcgatcga acggatcaca
      901 gtggtcacgg cggtctccaa acgaagttct caaacccgcc agcctcgcac gcagatgccc
      961 aaaagaaaag gcacgcgagc cttgcggcgc gcgtgccttt taactgcttg accgaagaca
     1021 aacaaagttg tcttcggtca tgcggctttt acatcttcac tacgtcgagc agcgtcttct
     1081 tgccgccctt atagttgtac agcgagatca cgccgtgctg aaggtcgccc ttcgagtcga
     1141 acgtggtttc gccgatcaca cccttatagt cggtcgccgg catggctgcc aggatcttcg
     1201 ccgggtcggt cgagttcgca cgcttcatag cgtcgacgat gatgtacaca gcgtcatacg
     1261 tgaacggagc gtagatctgg atcggctgac cgaaacgctt ctgatacttg gcgaggaacg
     1321 ccgggccgcc agccatcttc tcgagcgcca taccggcttc cgagcagacg atgttgtcgg
     1381 ttgcgtcgcc agccaggtcg gacagcttat ccgtacacac gccgtcgcca gccagcacct
     1441 tcgcgcgcag gccaagttgc ttggcttgct tggcgaacgg gccgccggtg gcgtccatgc
     1501 cgccgtacat gatcgcgtcc gggttttcgc ccttgatctt cgtcagaatc gcgcggaagt
     1561 cgacagcctt gtcgttcgtc gcgtcatgcg acatcacgtt caggcccagc gacttggcgg
     1621 tcttttcgaa ttcgtt
//
LOCUS       DATASH010000659         4347 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6238590,
            whole genome shotgun sequence.
ACCESSION   DATASH010000659 DATASH010000000
VERSION     DATASH010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4347
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6238590"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..1036
                     /locus_tag="VHV99_17745"
     CDS             161..1036
                     /locus_tag="VHV99_17745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX3637291.1"
                     /translation="MKPAVFSYHRPHTLEDALALLAQHGFDAKVIAGGQSLAPMMNMR
                     FAQPAHLIDLNALAQLRGVTLAGESLSVGAMTRHHEVATSSTVRAACPLLADAAATIG
                     HYAIRQRGTLGGSLAHADPAAQMPLIASTLGARVEVIGPDGSREVPAAEFLIAVMTTD
                     LAAEEIIRAVHFPVARPGERHAFEIFSRRHGDFAIAACALTAVLDGNRFSSLRIGLGG
                     VADAPLVLDELAASFAGAPVDAPTLAAIADAAAAAIDPYDQPDISAAYRRDLVKALTA
                     RALQRATQSARTTSN"
     gene            1040..1528
                     /locus_tag="VHV99_17750"
     CDS             1040..1528
                     /locus_tag="VHV99_17750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX3637292.1"
                     /translation="MEKQTIYLNVNDTRHEVHCEPRKLLSDVLREDCRLTGTHVGCEH
                     GVCGACTVQIDGRPARSCLAYAVQMDEARITTIESVAQDGRLSPLQQAMHECHGLQCG
                     FCTPGIVMTFEAYLREHPSPSEAEVRDVLSGNLCRCTGYQNIVQAILLAAERMRGETV
                     DV"
     gene            1521..3875
                     /locus_tag="VHV99_17755"
     CDS             1521..3875
                     /locus_tag="VHV99_17755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX3637293.1"
                     /translation="MSEQTFRYLNKAVPRTEDYRLLTGYGRYLDDIEIAGALHVSFVR
                     SPHAHARIVSVDTTAAAGMPGVVTVVTGRELAQWTTSMRVAPPIDGLLPVEFATMPTD
                     VVRFQGDLVACVVATDRYLAEDAAEQVMVEFEPLPVVTDMWQALEADAAQVDPGVPGN
                     LVSHQTYSNGDPQSASQQAYRVVEATFQQQRQTHVPIETRGCAAVWDAGRDHLTFHIG
                     TQVPHPLRTQLAHRLRLSESQVTVISPDVGGSFGQKIALYREELTVAALARHLKRPVR
                     WREDRLENLTAAANSREDFCRTRAAVDEHGTLLSLELEMREDFGAYCFFPANYLARVV
                     AMVLSGPYRLQNYAFDVKVVLSHKCGNAPMRAPMSITSWVMEGTMEAIARELKLDPVE
                     VRRRNLLTPEELPYSTATGEVLADITPSQTFEAVLEAFDYDAFRARQQAARAAGRYLG
                     LGLCNVVEPTTYGSRFYKAAGIPGSGHEASWIRIEPSGVVNASVGLGPSGQGYESAMA
                     NAVAEGLGVHPSSVRIHLGHTDIAPYGMGSRGARSGTAGGGALYLCALDARRKVLDIA
                     AGCLELPDSDSLRLVDARVERRNGDTWVDAGLTLADVARRAYMDPTSLPQGLTPGLEF
                     HLTYDPPPMTYSNSTHGCEIELDVATGAIHFARYVIAEDCGTLLNPTVVKGQQHGAVA
                     MGLSGALFEHMRYDETGQNVTGSLADYLVATSTELPNFEIIPMHTPNRATAAGIKGMA
                     EGGVMGALGALTNAVNDALAPFGVVVDRHPLTPMLLRDLLRDKL"
     gene            3891..>4347
                     /locus_tag="VHV99_17760"
     CDS             3891..>4347
                     /locus_tag="VHV99_17760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX3637294.1"
                     /translation="MNQSASKPAVGFIGLGIMGASMAARLAAAGYPLHVYNRTRAKAE
                     PLIEAGASWHDTPKALAEASDVVITIVGMPDDVRALYLGDDGLIANARPDAVLIDMTT
                     SSPQLAEELAAQAAARGVHMLDAPVSGGDIGAREGRLSIMVGGAADALAR"
BASE COUNT          737 a         1394 c         1435 g          781 t
ORIGIN      
        1 atcagtttat cgggctgcaa tgtttggggc agtccatgtt tttccgtgtc ggcaaggagc
       61 gttcctgcaa ggtcggtgaa cgcgtgaccc tgtcgatcaa ccgccgccgt ctgcaccttt
      121 tcgatcaggc gagcggcgcg aacctgaagg cgcagaacgc atgaaacccg cagtattttc
      181 gtatcaccgg cctcacacgc ttgaagatgc gctggcgctg ctcgcgcagc acggcttcga
      241 cgccaaagtc atcgcgggcg ggcaaagcct cgccccgatg atgaacatgc gctttgcgca
      301 gccggcacat ctgatcgacc tcaacgcatt ggcgcaactc cgcggcgtga cgcttgcggg
      361 tgagtcgctc tccgttggcg cgatgacgcg ccatcacgaa gtggccacca gcagcaccgt
      421 gcgcgccgct tgccccctgc tcgcggatgc ggccgcgact atcggacact acgcgatccg
      481 ccagcgtggc acgcttggcg gcagtcttgc gcatgcggat cctgctgcgc aaatgccgct
      541 catcgccagc acgctgggtg cgcgcgtcga ggttattgga ccggacggca gccgtgaggt
      601 gccggccgca gagttcctga ttgcggtgat gacaaccgat ctcgccgccg aagaaattat
      661 tcgcgccgtg catttcccgg ttgcacgccc gggcgagcgg cacgcatttg aaatctttag
      721 caggcggcac ggcgacttcg cgattgcggc ttgcgcactc accgccgtgc tcgacggcaa
      781 ccgattctcc agcctgcgca ttggccttgg cggcgtggcg gatgccccgc tggtgctgga
      841 cgaactcgcc gcgagcttcg ccggcgctcc ggtcgatgca ccgacccttg ctgcaatcgc
      901 cgacgcggcg gctgccgcta tcgatccgta cgatcaaccg gacatcagcg ccgcttaccg
      961 gcgcgatctt gtcaaggcgc tcacggcgcg cgcgttgcaa cgcgcgacgc agtcggcccg
     1021 cacgacctcg aattgagtca tggaaaagca aacaatttat ctcaatgtga acgacacccg
     1081 tcacgaagtg cattgcgagc ctcgcaagct gctttccgat gtgcttcgcg aagattgccg
     1141 tctgaccggc acgcacgtcg gctgcgagca cggcgtgtgc ggcgcatgca cggtgcagat
     1201 cgacggccgt ccggcgcgct cgtgcctcgc ctacgccgtt cagatggacg aagcgcggat
     1261 caccacgatc gaatccgtcg cacaggatgg ccgcctctcg ccgctgcagc aggcgatgca
     1321 tgagtgccat ggcctgcagt gcggcttttg cacgccgggc atcgtgatga ccttcgaggc
     1381 ttatttgcgc gagcacccca gcccatcgga ggccgaggtg cgcgacgtgc tttccggcaa
     1441 cctgtgccgc tgcaccgggt accagaacat cgtgcaggcg attctgcttg cggctgagcg
     1501 gatgcgcggg gagaccgtcg atgtctgagc agacgtttcg ctacctcaac aaggctgtgc
     1561 cgcgcaccga agactatcgg ctgctgaccg gctatggccg ctatctcgac gatatcgaaa
     1621 tcgcgggcgc tctgcatgtg agcttcgtgc gctcgccgca tgcgcacgcg cgaatcgtgt
     1681 cggtggatac cacggcggct gccggcatgc ctggtgtcgt gaccgtcgtc accggccggg
     1741 aactggcgca gtggaccacg tcgatgcgtg ttgccccgcc gatcgacggc ttgttgcctg
     1801 tcgagttcgc gacgatgccg accgacgtcg tgcgctttca gggcgatctg gtggcgtgtg
     1861 tggttgcgac cgaccggtat ctcgcggaag acgcggcgga acaggtcatg gtcgagttcg
     1921 agccgttgcc tgtggtgacc gatatgtggc aagcgctcga agccgatgcc gcgcaagtcg
     1981 accccggcgt gcccggcaac ctggtgtcgc accagacgta ctcgaacggc gacccgcaga
     2041 gcgcgagcca gcaagcttac cgcgtggtcg aggcgacctt tcagcagcag cggcagaccc
     2101 atgtgcccat cgagacgcgc ggttgcgcag cagtctggga cgccgggcgc gaccacctga
     2161 cgttccacat cggaacccag gtgccgcatc ctttgcgcac gcagctcgcg caccggctca
     2221 ggctcagcga gtcgcaggtg acggtaattt cgcctgacgt cggcggttcg ttcgggcaaa
     2281 aaatcgcctt gtaccgcgaa gagctgaccg tggccgcact cgcgcgccat ctgaagcggc
     2341 cggtgcgctg gcgcgaagac cgcctcgaga atctgaccgc agcggccaat tcgcgcgaag
     2401 acttctgccg tacgcgcgcc gcggtggatg agcacggcac gttgctctcg ctcgagctgg
     2461 agatgcgcga agacttcggc gcgtattgct tctttccggc gaattacctc gcgcgcgtcg
     2521 tcgcgatggt gttgagcgga ccgtaccggc tgcagaacta cgcgttcgat gtgaaggtcg
     2581 tgctcagtca taaatgcggc aatgcaccca tgcgcgcgcc gatgtccatc accagttggg
     2641 tcatggaagg cacgatggaa gcgattgcgc gcgaactgaa gctggatccg gttgaggtgc
     2701 gccgccgcaa cctgttgacg ccggaagaac tgccgtacag cacggccacc ggcgaagtgc
     2761 tggccgacat cacgccgtcg cagacctttg aggcggtgct ggaggccttc gattacgacg
     2821 cgtttcgagc ccgccagcag gcggcgcggg cggccggccg ctatcttggg ctgggcctgt
     2881 gcaatgtggt cgagccgacc acgtacggct cgcgctttta taaagcggcc ggcattccgg
     2941 gctcgggaca cgaggcaagc tggatccgta tcgagccgag cggtgtcgtg aacgcgtcgg
     3001 tgggtctcgg ccccagcgga cagggctatg aaagcgcaat ggcgaatgcg gtggccgaag
     3061 gcctgggtgt gcatccgtcg agcgtgcgta ttcatctggg tcacacggat atcgcgccat
     3121 acgggatggg cagccgcggc gcccgcagcg gcacggcggg cggcggcgcg ctttatctgt
     3181 gcgcgctcga tgcacgccgc aaggtgctcg acattgccgc cggttgtctc gaactgcctg
     3241 atagcgactc gcttcgcctt gtcgatgcgc gcgttgagcg ccgtaacggc gacacgtggg
     3301 tcgatgcggg tctcacgctc gccgacgtgg cgcggcgtgc ctatatggac ccgacttcgc
     3361 tgccgcaggg cctcacgcct ggcctcgagt ttcacctgac ctacgacccg ccgccgatga
     3421 cctactcgaa ttcgacgcac ggctgcgaga tcgaactgga cgtggccacg ggtgcgatcc
     3481 attttgcgcg ctacgtgatc gccgaagact gcggcacgct gctcaatccc accgtggtga
     3541 aagggcagca gcatggtgcc gtggcgatgg ggctgtccgg cgcgttgttc gagcatatgc
     3601 gctacgacga aacgggacag aacgtgaccg gctcgctggc cgactacctg gtcgccactt
     3661 cgaccgaatt gcccaatttc gaaatcatcc caatgcatac gcctaaccgc gcgacggcgg
     3721 cgggtatcaa agggatggct gaaggcggcg tgatgggcgc gctcggtgcc ttgaccaacg
     3781 ccgtgaatga cgcgctagcg ccattcggcg tggtggtgga ccgtcatcct ttgaccccca
     3841 tgctgttgcg cgacctgctg cgcgacaaac tctgactgga tcaaacaatc atgaatcaat
     3901 ctgcaagcaa acccgctgtc ggctttatcg gcctcggcat catgggcgcc tcgatggcgg
     3961 cgcggctcgc tgcagccggc tatccgctgc atgtctataa ccgtacccgt gcgaaggccg
     4021 agccgctcat cgaggcgggc gcgtcctggc atgacacccc gaaggcgctc gctgaagcct
     4081 ccgacgtggt catcacgatc gtcggcatgc cggacgacgt gcgtgcgctg tacctcggcg
     4141 acgacggtct gatcgcgaac gcgcggcccg atgccgtgct gatcgacatg acgacttcga
     4201 gcccgcaact ggccgaagag ctggccgcgc aggctgcagc gcgcggcgtg cacatgctgg
     4261 acgcgccggt ttcgggcggc gatatcggcg cgcgcgaagg acggctgtcc atcatggtgg
     4321 gcggcgcggc cgacgcgctc gcgcgcg
//
LOCUS       DATASH010000660         2316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1295883,
            whole genome shotgun sequence.
ACCESSION   DATASH010000660 DATASH010000000
VERSION     DATASH010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1295883"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..422
                     /locus_tag="VHV99_17765"
                     /pseudo
     CDS             1..422
                     /locus_tag="VHV99_17765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431390.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
     gene            419..1123
                     /locus_tag="VHV99_17770"
     CDS             419..1123
                     /locus_tag="VHV99_17770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3637295.1"
                     /translation="MKGMVQCVVMRLAVTAALMATLTAYAQDNRVAKSDDPETQTAVA
                     DYNAGNLGAALAEFRKAAEHGSRLAEFNYAMMLLNGEGTTANVDEGKKWLRKAADANM
                     SHAQYVYGKMYDDGEFVGRDPVEAHRWFLKAAQQGHVQAELALANQFLDGRGTARDNR
                     QAFVWYKKAAQGGDMTAQYVTGSFYERGGDGVDKNLNVARAYYAAAAVQGDPAAKLKY
                     QQLSAQLRDQKEVKPQ"
     gene            complement(1319..>2316)
                     /gene="rodA"
                     /locus_tag="VHV99_17775"
     CDS             complement(1319..>2316)
                     /gene="rodA"
                     /locus_tag="VHV99_17775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004525857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEX3637296.1"
                     /translation="QMRNIMLTFVLMWALANVPPTTLMRFAVPLYTFGIALLIAVAVF
                     GLTRKGAKRWINVGVVIQPSEILKIATPLMLAWYYQRREGVMRWYDFLVGFLILIVPV
                     GLIAKQPDLGTAVLVFAAGFFVIYFAGLSFKLIVPVLIAGVIAVGSIAAFQDKICQPQ
                     VQWPLMHDYQKHRICTLLDPTSDPLGKGFHTIQAVIAIGSGGPLGKGWLKGTQAHLEF
                     IPEKHTDFIFAVFSEEFGLAGGIVLLTLYMLLIARGLYIAANGATLFGRLLAGSLTMA
                     FFTYAFVNIGMVSGILPVVGVPLPFMSYGGTALTTLGVAIGLIMNVARQKRLMQS"
BASE COUNT          478 a          697 c          750 g          391 t
ORIGIN      
        1 gctcgacggt cagcgcggcg tcgccggcgc ggtgcgggca gctgcgtacg gcatgcggcg
       61 cgactacgtg cagacggcca acgcacagat ttcgaccatc gtgcagatcg agtcggcgcg
      121 cggcttgcag gaagtcgaga agatagcgtc gacgccgggc gtcgattgcc tgtttgtcgg
      181 accggccgat cttcgcgagc ctcggtcatc tcggcgattc gaagcacgct gacgtgcagg
      241 ccgcgatggc acgtattgtc agcgccgccg agaaggccgg tatcgctgcg ggaatttttg
      301 ccatggacgt cgcgagcgca aagcagcatc gcgatgccgg ttttcgtttt atcgccctgg
      361 ccgccgacgt catgtggatg ttgcgcgcga cccgccaggc gttgcaggag gtgaggacat
      421 gaaggggatg gtgcagtgcg tcgtgatgcg tctggcggtc acagcggcgt tgatggcgac
      481 cttgacggcg tacgcgcagg acaatcgcgt cgcgaagtcg gacgatccgg agacgcaaac
      541 cgcggtcgcc gattacaacg ccggcaatct gggcgcggcg ctggccgaat tccgtaaagc
      601 ggccgagcac ggcagccggc ttgccgagtt caactacgcg atgatgctgc tcaacggcga
      661 aggcaccaca gccaatgtcg acgaaggcaa gaagtggctg cgcaaagccg ccgatgccaa
      721 catgtcgcac gcgcaatatg tctacggcaa gatgtacgac gacggtgagt tcgttgggcg
      781 cgatccggtg gaggcacatc ggtggttcct gaaggccgcg caacaggggc acgtgcaggc
      841 ggaactcgcg cttgccaacc agttcctcga tggccgtggc acggcgcgtg ataacaggca
      901 ggcgttcgtc tggtacaaga aggccgcgca aggtggcgac atgaccgcgc agtacgtgac
      961 cggctcgttc tacgagcgcg gtggcgacgg tgtcgacaag aacctgaacg tggcgcgcgc
     1021 gtattatgcg gcggcggcgg tgcagggcga tccggcggcg aaactcaagt atcagcaact
     1081 gagcgcgcaa ttgcgggatc agaaggaagt gaaaccgcag taaatgaaca aggggcgctc
     1141 ccgacggtgc gccccttttt gcatgtccgg cctactttct gcgcctgctt tcgcgggtcg
     1201 aatacggcgg tgtcgaagaa gcgggagcgt cgtttccgtt gcggatgacc gatgggactt
     1261 tctcctgccg aagggttttg tgctgcgttt agcaccgcag cctgagcccc gcgcaccgct
     1321 aactctgcat caaccgcttc tgacgcgcga cgttcatgat gagaccgatg gcgaccccta
     1381 gcgtagtcag cgccgtgccg ccgtaactca tgaacggcaa cggcacgccc accaccggca
     1441 agatcccgct caccatgccg atgttgacga acgcataggt gaagaacgcc atcgttagcg
     1501 atccggccag tagccgcccg aacagcgtcg ccccattggc cgcgatatac aaaccgcgcg
     1561 cgatcagcag catataaagc gtgagcagca cgatcccgcc cgccagcccg aactcttcgg
     1621 aaaacaccgc gaagatgaag tcggtgtgtt tctcggggat gaactccaga tgcgcctgag
     1681 tacccttgag ccagcccttg ccgagcgggc cgcccgagcc gatcgcaatc acggcctgaa
     1741 tggtgtggaa gcccttgccg agcggatctg acgtcggatc gagcagtgta cagatacggt
     1801 gtttctgata gtcgtgcatc agcggccact gcacttgcgg ctgacagatc ttgtcctgga
     1861 acgccgcgat cgagccgacc gcgatcacgc cggcaatcag caccggcacg atcagcttga
     1921 aactcagccc cgcgaagtag atcacgaaga agccggccgc gaacaccagc acggcagtgc
     1981 caaggtcagg ctgcttggcg atcaaaccga ccggcacgat cagaatcaga aagccgacca
     2041 gaaagtcgta ccagcgcatc acgccttcgc gtcgctggta gtaccaggcg agcatcagcg
     2101 gtgtggcgat cttcagaatt tccgacggtt ggatcaccac gccgacgttg atccagcgct
     2161 tcgcgccctt gcgtgtgagg ccgaacaccg ccacagcaat cagtaacgcg atcccgaacg
     2221 tatagagcgg gaccgcgaag cgcattagcg tggtgggcgg cacgttggcc agcgcccaca
     2281 tcagcacgaa cgtcagcatg atgttgcgca tctggt
//
LOCUS       DATASH010000661         4963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1691963,
            whole genome shotgun sequence.
ACCESSION   DATASH010000661 DATASH010000000
VERSION     DATASH010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4963
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1691963"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VHV99_17780"
     CDS             <1..475
                     /locus_tag="VHV99_17780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006405185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX3637297.1"
                     /translation="RLSHAPSSDIGSDRLVPNELVPVCSPDFLRTHPDLNDATPETLS
                     NVTLLHSLARREDWAKWLDEAEVRGVNPLGGLSYESSVLAYAAAMQGHGVAIAQRVLV
                     ANQLRDGTLVMPFSFILDLGAFTYYLVYPHEHLQKPEFAAFRDWLLSVSAASPDA"
     gene            complement(472..1275)
                     /locus_tag="VHV99_17785"
     CDS             complement(472..1275)
                     /locus_tag="VHV99_17785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007737650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX3637298.1"
                     /translation="MTVHYSKSEHVAIVTLDRPESLNALNLDSLKALRAALVTARDDD
                     DVRAIVLTGSGNKSFCVGADLKNTLPPETSFSSSFVRPIDRAAAEGIYVRMMDLTSLR
                     LFKPMIAAINGYCLGGGLELALQCDLRIASDNAVFGLPEPVVASIPAVCGIQALQKAV
                     PSAIAMKMLYTGSRIDAAYAERVGLVSDVVAPAALMDKALQIAGAIAANGPLAVQMIK
                     KIVETSSNVPLAQAIEFTELAWGAMRDSEDRVEGRKAFAEKRSPRFKGR"
     gene            complement(1287..2057)
                     /locus_tag="VHV99_17790"
     CDS             complement(1287..2057)
                     /locus_tag="VHV99_17790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007583082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3637299.1"
                     /translation="MNASHQSSDAAETAIVTGGAKGIGFGIAQALARHGRRIALFDLD
                     RAALDRASTELAASGVEVIGLPVDVTNGESVNQAVDAVIERFGRIDVLVNNAGIVRDK
                     RITKMSDEDWDIVIDVNLKSQFLCCRAVLAHMATARYGRIVNIASRAWLGGAGQSNYS
                     ASKGGVVSLTRSLALEWASAGITVNAVAPGIVDTPLFRTFDAALQERLQKSVPAQRIG
                     TPDDIAQAVSFFALREASYITGQTLYVCGGRSLSSPSV"
     gene            2303..3604
                     /locus_tag="VHV99_17795"
     CDS             2303..3604
                     /locus_tag="VHV99_17795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007737648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3637300.1"
                     /translation="MDTTLPGVAEPSAAALTGAQKRAIVAATLGTVVEFADWIIYATF
                     AALFSRHFFPAHDERISLLSAFAVFAVGFIMRPIGGALLGAYADRHGRKNGLALSVAL
                     MAGSSLVIAACPSYQSIGFTAPVILVLARLVQGFAAGGEFGSAATFLIESSAPSRRGF
                     AGSWQHFAVNAGVLVAASIGALLTSIIQPAGMAEYGWRIAFGIAGLAGFVALWVRLAV
                     AETDAFKKVARHAHVLHPFISIVRDHRRAALRVVGIAMAGNLCIYLWLVLFPTLAHLR
                     TGLPLSAAFRASVISIVVSLIAIPFIGMLSDRIGRKPVLLAFAGGSALFAWPALSLLS
                     NDFWNATVIVTIGMVLSSGFAATCATVMAEQFPAHVRATGVALPYAISAALFGGTLPY
                     IVTAMCNSGLSQYLWVYVAAVCAVGFVVYARMPETRGKILD"
     gene            3627..4799
                     /locus_tag="VHV99_17800"
     CDS             3627..4799
                     /locus_tag="VHV99_17800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007737647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX3637301.1"
                     /translation="MRKVVIGGVGMTSFGKFLERNLKSLSEEAVALALKDARVSANDV
                     DRIFFGNAAAGVVTGQEMIRAQSSLRNTGLDGKPMFNVENACASGSSALNLAWWSVAS
                     GQSETALIVGVEKLSHADKAISFGAFAKAVDLEEPLPEGITTGTGSLFMDLYAAKARK
                     WMEKSGADASDFARVVVKSRRAGSLNPVAQFRKETTVDEVLASRMVSDPLTLFMCSSI
                     GDGGAALFICSEDYARRHDMPPVFIRASSIVSAKADGSGELVAVRAAREAYEAAGIGP
                     EDVHVVELHDASAPAELIHYENLGLCAPGDAPKLIRSGDTDIGGRVSVNPSGGLLSRG
                     HPVGATGVAQIVELTQQLRGTAGARQRPGARVALAENNGGQLAGDSAVALVTILSM"
BASE COUNT          881 a         1529 c         1539 g         1014 t
ORIGIN      
        1 tcgtctgtcg catgcgcctt cgtccgatat cggctcggac cgcctggtgc cgaacgaact
       61 cgtgccggtg tgcagccccg acttcttgcg tacgcatcct gatctcaacg acgccacgcc
      121 cgaaacgctc tccaacgtga cgcttctgca ttcgctggca cgtcgcgagg actgggcgaa
      181 atggcttgat gaagcagaag tgcgcggcgt gaatccgctc ggtggactca gttacgaaag
      241 ctcggtgctc gcgtacgctg cggcgatgca ggggcacggc gtcgcaatcg cgcagcgcgt
      301 gctcgttgcc aatcagcttc gtgatggcac gctcgtcatg cctttctcgt tcatcctcga
      361 tctcggcgcg ttcacgtact accttgtcta tccgcacgaa catctgcaaa agcctgagtt
      421 cgccgcgttt cgcgattggc tgctttccgt gagcgcggca tcgcccgatg cttaacgtcc
      481 tttgaagcgc gggctgcgct tctcggcgaa tgccttgcgt ccttcgacgc gatcttccga
      541 atcgcgcatc gcgccccagg cgagttccgt gaactcgata gcctgcgcga gcggcacgtt
      601 gctgctcgtc tcgacgatct tcttgatcat ctgcacagcg agcgggccgt tcgccgcgat
      661 cgcgcctgct atttgcagcg ccttatccat cagcgcggct ggcgcgacca cgtcggacac
      721 gagaccgacc cgttcggcgt aagccgcgtc gattcgcgag ccggtgtaca gcatcttcat
      781 cgcgatggcg gacggcacgg ctttttgcag cgcctgaatg ccgcacaccg ctggaatgct
      841 cgcgacgacc ggctcaggca agccgaacac cgcgttgtcc gatgcaatcc gcagatcaca
      901 ttgcaatgcg agctccagtc cgccgccgag gcaataaccg ttgatcgcgg caatcatcgg
      961 cttgaagagg cgcagcgatg tcaggtccat catccgcacg taaatgcctt cggccgcggc
     1021 gcgatcaata ggtctcacga acgatgaaga aaagctcgtt tcgggtggca atgtgttctt
     1081 gagatccgca cccacgcaga aggacttgtt ccctgatccg gtcagcacga ttgcgcgcac
     1141 gtcgtcgtca tcgcgtgccg tgacgagcgc agcgcgcagt gccttgagac tatcgaggtt
     1201 gagtgcgttg agcgactccg ggcgatcaag cgtgacgatc gcgacgtgct ccgatttgct
     1261 gtagtgaaca gtcatgatgc actccgtcaa acgctgggcg acgacaacga acgcccgccg
     1321 cacacataaa gcgtttgtcc ggtgatgtac gacgcttcac ggagcgcaaa gaacgacacg
     1381 gcctgcgcga tatcgtcggg cgtgccgata cgctgcgcgg gtacggactt ctgcaggcgc
     1441 tcctgaagtg cggcatcgaa ggtgcggaaa agcggcgtat cgacgatccc tggcgcaacc
     1501 gcgttgaccg tgatgcccgc actggcccat tcgagcgcga ggctacgcgt gagactcacg
     1561 acgccgccct tggatgccga atagttcgac tggcccgcac cgccgagcca ggcgcgcgaa
     1621 gcgatattga cgatgcgccc atagcgcgcc gtcgccatat gcgcgagtac cgcgcggcag
     1681 cacaaaaact gcgacttcaa attcacgtcg atgacgatgt cccagtcttc gtcgctcatc
     1741 ttcgtgatgc gcttgtcgcg cacgatgccg gcgttgttca ccagcacgtc gatgcgtccg
     1801 aagcgctcaa tcacggcatc gacggcctgg ttcacggatt cgccgttcgt cacgtcaact
     1861 ggcaagccga tgacttcgac gccggaagct gcgagttcgg tcgacgcccg atccagcgcg
     1921 gcacgatcga gatcaaagag tgcaatgcgc cgcccgtgac gcgccagcgc ctgcgcgata
     1981 ccgaagccga tgcccttggc accgcccgtg acgatcgcgg tctcggcggc gtcggatgat
     2041 tgatgtgaag cgttcatgaa atgtctccgt ggaatccagt cttcaagctg tggattccat
     2101 gctagggcgc gcggcccatc cgcttcaaac gacttttttc ggaacttcga ttgacttgcg
     2161 gtcaatcgag aacggccgac ttcgatacag aaaaaatcaa tcgaaaacgc agaaaaagtc
     2221 gtttgcccga cgcgcgtcag gaaaactacg atgcattcat gtgccaccgg gcacgttcat
     2281 atcaatcgac acggagacaa gcatggatac cacccttccg ggcgttgcag agccatccgc
     2341 cgcagcgctc acgggcgctc agaaacgagc gatcgtcgcg gcgacgctcg gcaccgtcgt
     2401 cgaattcgcg gactggatca tttacgccac attcgccgcc ctcttttcgc ggcatttctt
     2461 tcctgcgcac gacgaacgca tatcgctcct gtctgcgttc gccgtgttcg cggtcggctt
     2521 catcatgcgg cccatcggcg gcgctttgct tggcgcttat gcggaccgtc acggtcgcaa
     2581 gaatggcctc gcgttatctg ttgcgttgat ggccggcagt tcgctcgtga tcgccgcttg
     2641 tcccagttac caatcgatcg gcttcaccgc accggttatc ctcgtgctcg cgcggctcgt
     2701 gcaaggcttc gcggcgggcg gcgaattcgg ctcggcagcg acatttctga ttgaatcgtc
     2761 cgcgccttcg cgacgaggct tcgcagggtc gtggcagcac tttgcggtca acgcgggcgt
     2821 gctcgtggct gcatccatcg gcgcgcttct gacttcgatc atccaacctg ccggtatggc
     2881 ggaatacggc tggcgcatcg cgttcggcat cgcggggttg gcgggtttcg tcgcgttgtg
     2941 ggtacggctt gcggtcgccg aaaccgacgc attcaagaag gtcgccaggc acgctcacgt
     3001 gctgcatccg tttatctcga tcgtgcgcga tcaccggcgc gcggcgctgc gcgtggtggg
     3061 catcgcgatg gcgggcaatc tctgcatcta tctctggctc gtgctgtttc cgacgctcgc
     3121 gcatctgcgc accggtctgc cgctttccgc cgcgtttcgc gcaagcgtga tttcgatcgt
     3181 cgtatcgctg atcgcaattc cgttcatcgg catgttgtcg gatcgcattg gacgcaagcc
     3241 ggttctgctc gcgttcgccg gcggctcggc gctctttgcg tggcccgcgc tgagtctgct
     3301 gtcgaatgat ttctggaatg cgaccgtcat cgtcacgatc ggcatggtgc tgtcgtcggg
     3361 attcgccgcg acgtgcgcga cggtgatggc cgaacagttt cccgcgcacg tgcgcgcaac
     3421 gggcgtcgcc ctgccgtatg caatctcggc agcgctcttt ggcggtacgt tgccctacat
     3481 cgtcaccgcg atgtgcaact cgggtctgtc gcaatacctg tgggtctatg tcgccgctgt
     3541 gtgcgccgtg ggcttcgtcg tgtatgcgcg catgccggag acgcgcggaa agatcctcga
     3601 ttgacgctta ttttctggag aaagcaatgc gaaaagtagt catcggcggc gtcggcatga
     3661 cgtcattcgg gaagtttctc gaacgcaatc tgaagtcgct ttccgaggaa gcggtcgcgc
     3721 tcgcactcaa agacgcgcgc gtaagcgcga acgacgtgga ccgaattttc tttggcaacg
     3781 ctgcggcggg cgtcgtgacg gggcaggaaa tgattcgcgc gcaatcgtcg ttgcgtaaca
     3841 cgggactcga cggcaaaccg atgttcaacg tggagaacgc gtgcgcatcg ggcagctcgg
     3901 cgctcaatct tgcgtggtgg tcagttgcat cgggacagtc ggagacggcg ctgattgtcg
     3961 gcgtcgaaaa gctttcgcac gcagacaaag cgatttcgtt cggcgcgttc gcaaaagccg
     4021 tcgatctcga ggagccgctg ccggaaggca tcaccacggg caccggatcg ctcttcatgg
     4081 atctctatgc agcgaaagcc cgcaagtgga tggaaaagag cggtgccgat gccagcgatt
     4141 tcgcgcgcgt ggtcgtgaag agccgccgcg cgggatcact gaatccggtc gcgcagttcc
     4201 gcaaagaaac gaccgtcgat gaagtactcg cgagccgcat ggtcagcgat ccgctgacgc
     4261 tcttcatgtg ctcatcgatc ggcgatggcg gggcggcctt gttcatctgc tccgaagact
     4321 atgcgcgcag gcacgacatg ccgcccgtgt tcatccgcgc gagttcgatc gtctccgcca
     4381 aggcggatgg ctcgggtgaa ctggtcgccg tgcgcgcggc cagagaggcg tacgaggcgg
     4441 cgggcatcgg accggaagac gtgcatgtcg tcgaattgca cgatgcatcc gcgcccgccg
     4501 agttgattca ttacgagaac ctcggcctgt gcgcccccgg cgatgccccg aagctcatcc
     4561 ggtccggcga tacggatatc ggcgggcgcg tgtcggtgaa cccgagcggc ggcttgctgt
     4621 cgcgaggtca tccggtgggc gcgacgggcg tggcgcagat cgtcgaactc acgcagcaac
     4681 tgcgcggcac agcgggcgcg cggcagcgtc cgggcgcccg cgtggcgctt gctgagaaca
     4741 atggcggcca gctcgcggga gattccgccg tcgcgttggt aacgattcta tccatgtgag
     4801 tttgcgcaag tgccgaacgt ctcgttcaac ggtcggcact tttcgctttt aaagacgcct
     4861 gttgctgaat gatcttgctc agaaaatcct tcgtgcgcgc ctgccgcgcc atcggactgc
     4921 cgaagaactc atcgcgcgat tacacaggga tcgaatggca gat
//
LOCUS       DATASH010000662        11718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1692146,
            whole genome shotgun sequence.
ACCESSION   DATASH010000662 DATASH010000000
VERSION     DATASH010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 11718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11718
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1692146"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(220..813)
                     /locus_tag="VHV99_17805"
     CDS             complement(220..813)
                     /locus_tag="VHV99_17805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX3637302.1"
                     /translation="MTNKPAPTAVAIHELIAGRWSPRAYSSEPVSREHLHAVLEAARW
                     APSSYNAQPWRFLVFDRSVDEVSFKQAFATLVPFNQGWNAPAPVLIAVTTHTLTNKGE
                     VNRCAPYDAGAAAMALVLQAHALGLAAHQMSGFDPNAFRATFKLPNDVDVIAMIALGH
                     YGDVDKLDPVLREREKSVRQRLPLADIAYGGGWKKAF"
     gene            complement(1000..2283)
                     /locus_tag="VHV99_17810"
     CDS             complement(1000..2283)
                     /locus_tag="VHV99_17810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3637303.1"
                     /translation="MNWAARLIGGRFHYGWLTVAVVFLVLLAAAGTRATPSVMMVPLE
                     HQFGWSRATISLAISVNIALYGLMGPFAAAAMQRFGVRPTLLIALGTMAAGVALSSLM
                     THPWQMVLIWGAMVGGSTGVAALSLSATVVTRWFTTHRGLVMGILTASSATGQLVFLP
                     MLAAVAEHYGWRQVVWVVAGAAAVVLPLVAFLLPERPADMKLRPFGEPADAPISATVT
                     KQNPLAIAFGTLAMASKTRDFWLLFFSFFICGASTNGYVGTHLIAMCGDYGMTEVQGA
                     SLLAAMGIFDLFGTTLSGWLSDRFNSRVLLFWYYGLRGLSLMYLPHAFGIDFFGLPLF
                     AVFYGLDWIATVPPTVRLATDVYGKEAAPVVFGWVVAGHQLGAAFAALGAGMLRASLG
                     TYTVASMISGGLCLVAAVIVLRINRPAKVPEPQAV"
     gene            2438..3079
                     /locus_tag="VHV99_17815"
     CDS             2438..3079
                     /locus_tag="VHV99_17815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX3637304.1"
                     /translation="MSDSETLKPAGARRARGAQTAGPQAQQHLLRAADELFYREGVRA
                     VGVDAVVERAGVNKMSLYRQFSSKDELVMAYLERKDEQFFGYVEKSFAKHPGEPAKQL
                     QQYFDELAVRASIDDYRGCPFVNVSVEFPDATHPARQFVFHNKARLMARLLELTTATG
                     ADDPVALANALGLMIEGVYAASQTYGPGCGPILAAPKVAAQLIAAACSGTQAG"
     gene            3173..3727
                     /locus_tag="VHV99_17820"
     CDS             3173..3727
                     /locus_tag="VHV99_17820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006052309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HEX3637305.1"
                     /translation="MPLPADSHDAVIAATRHWLTEAVIGLNLCPFAKAVHVKGQIRYA
                     ISDAVNMEGVLTDLETEIQALVAENPETVDTTLLVLPQALGDFLDYNDCLFFADRLIK
                     QLRLEGIIQIASFHPHYQFEGSEPDDIENYTNRAPYPILHLLREKSIERAVQAFPDAE
                     DIYERNQETLRRIGLRGWNDLMKM"
     gene            complement(3769..4611)
                     /locus_tag="VHV99_17825"
     CDS             complement(3769..4611)
                     /locus_tag="VHV99_17825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012430638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX3637306.1"
                     /translation="MSSKTHEIRPNQSVELLKELHILTRDGKMNQDSRRKLKQVYHLF
                     QFIEPLLKELKDQQGAVTLVDHGAGKSYLGFILYDLFFKEFQDAGGGASHIYGIETRE
                     DLVTKSEELANRLGFKGMSFLNLSVAESITSARLPRQIDIVTALHACNTATDDALRFA
                     LEKRAKYIVVVPCCQAEVAGVLRQNKSKSLGNALTEIWRHPLHTREFGSQITNVLRCL
                     QLEAHGYQVSVTELVGWEHSMKNELIIAQFKDLPRRRPAERLNEVMERLGIEELKERF
                     FVAA"
     gene            5007..5327
                     /locus_tag="VHV99_17830"
     CDS             5007..5327
                     /locus_tag="VHV99_17830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEX3637307.1"
                     /translation="MDTVPNGNVEQKFQEMLAKLTAAPAWSEKQQLELEMARDISSEM
                     LRLAEVMRDGSVDMETCLTMLKYAKVLDFVMTTLASRRDIKPQTLRVIFKLAGLKVDE
                     AYPG"
     gene            complement(5470..5679)
                     /locus_tag="VHV99_17835"
     CDS             complement(5470..5679)
                     /locus_tag="VHV99_17835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HEX3637308.1"
                     /translation="MTRKYIDCREFPSETNCTVALSADSENELLDAAVQHAVTVHKHT
                     DSPELRSQLKTLFHDGTPPIEAPRA"
     gene            6051..7328
                     /locus_tag="VHV99_17840"
     CDS             6051..7328
                     /locus_tag="VHV99_17840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HEX3637309.1"
                     /translation="MSSSFNSARVAVIGGGPAGLMAAEALARHGVQVDVHDAMPSVGR
                     KFLMAGKGGMNITHSEPLEPFLGRYGARREQIEPLLGMFGPDALRAWLQGLGVDTFVG
                     SSGRVFPADMKAAPMLRAWLHRLRETGVHFHMRHKWTGWNHDSGVGHAAAHALRFETP
                     GGEQIVTCDAVVFALGGASWPRLGSDAAWVPLLNAREVPVTPLRPANCGFDADWSPYL
                     RERFAGQPVKSVAITLTDVDNKVHNRQGEILLTETGLEGSLIYALSAAIRERILADGD
                     VTITLDLAPGLTLERVVAEVTRPRGSRSMSTHLHGRVGISGVKLALLHEMLSKEAFAD
                     ANRLAHAIKALPVRLTRARPIAEAISTAGGVPFEALDEHLMLKRLPGAFCAGEMLDWE
                     APTGGYLLTACFASGLVAGRGALAFLEARGLPV"
     gene            complement(7371..8495)
                     /locus_tag="VHV99_17845"
     CDS             complement(7371..8495)
                     /locus_tag="VHV99_17845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3637310.1"
                     /translation="MTSPATITWPEADGPRTARWRSEAAVPPPRRVVVADDRTTADSA
                     YRLACEGTALLWNGDFQNARQLLQAVTRRLERKPRKQGETPIDAFNLHRQAQSQRART
                     LGMILIPLDAAYGIPLRRAPEVQQACIETYGPSTDEPSVVSLRELLGLIGAHEWRKKG
                     VEIPALGDRIHPHYGVFSPVRGEYVDLVARTPLPSLNKAFDIGTGTGVLAALLARRGV
                     KKIVATDQDPRALACARENLTRLGYDQHIEVVQADLFPEGRAPLVVCNPPWVPARPAS
                     PIEYAIYDSESRMLLGFLNGLAEHLSPGGEGWLIMSDFAEHLGLRTREWLLAAIEKAG
                     LTVVGREDIRPRHPKSTDETDALHTARVAELTSLWRLKAR"
     gene            complement(8843..8918)
                     /locus_tag="VHV99_17850"
     tRNA            complement(8843..8918)
                     /locus_tag="VHV99_17850"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8883..8885),aa:Thr,seq:tgt)
     gene            complement(8938..9468)
                     /locus_tag="VHV99_17855"
     CDS             complement(8938..9468)
                     /locus_tag="VHV99_17855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HEX3637311.1"
                     /translation="MQEISTKPYLLRALYEWCTDNGYTPHIAVRVDNQTRVPRQFVRD
                     NEIVLNISFEATSQLQMGNEWIEFSARFSGKSHKIEVPIANILAIYARENGQGMAFPV
                     ESAGGEAQDSGADAELADETEAPAAPRATAPRAVESSSTAADAAAAKAETATDGQQPD
                     DDGSKGGGRARLKIVK"
     gene            complement(9543..10154)
                     /locus_tag="VHV99_17860"
     CDS             complement(9543..10154)
                     /locus_tag="VHV99_17860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006999756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX3637312.1"
                     /translation="MMVLYSGTTCPFSQRCRLVLFEKGMDFEIRDVDLFNKPEDIAVM
                     NPYGQVPILVERDLILYESNIINEYIDERFPHPQLMPADPVQRARARLFLLNFEKELF
                     VHVGTLENEKGKAAEKNHEKARLAIRDRLTQLAPIFLKNKYMLGEEFSMLDVAIAPLL
                     WRLDHYGIELSKNAAPLMKYAERIFSRPAYIEALTPSEKVMRR"
     gene            complement(10249..11004)
                     /locus_tag="VHV99_17865"
     CDS             complement(10249..11004)
                     /locus_tag="VHV99_17865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011350743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEX3637313.1"
                     /translation="MKKLLSTCALIGATLLAVLAGPAHADENFPLDRAPDNAENFASL
                     QHGAQIFVNYCLNCHSANLMRYNRLTDLGITPNEIQANLLFTTDKVGNTMTVAMRPDD
                     AKAWFGAAPPDLSVEARARGKDWLYTYLRSFYRDNTRPTGWNNLVYENVSMPHVLWQL
                     QGERTAKFGDAVDEKTGETVHKFVGFQQVTPGTMSPVDYDSTVADLVSYLSWMSEPTQ
                     KTRKQLGVWVLLFLGILSFFAWRLNAAYWKHIK"
     gene            complement(11027..>11718)
                     /locus_tag="VHV99_17870"
     CDS             complement(11027..>11718)
                     /locus_tag="VHV99_17870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HEX3637314.1"
                     /translation="NKGPDGVPLDGIPFHPYYSVHDFMGVTVFLLIFAVIIFFAPEMG
                     GYFLEANNFIPANPLQTPQEIAPVWYFTAFYAMLRATTDPFKIVLMIIVALLGLFALV
                     RARGKWKLGLPVLAVLVVLAMAFTESKFWGVVVMGGAVISLFFLPWLDRSPVKSIRYR
                     PFFHKVFYRIFVLAFLTLGFLGTNPPSPASTVIAQVCALIYFAFFLGMPVWTRLGTFK
                     QPPERVRFKPH"
BASE COUNT         2382 a         3618 c         3522 g         2196 t
ORIGIN      
        1 cggacggcag gtaatgaacc gcgcgccttg agtaccacgg gcggcaggga ataaaccgtg
       61 cgccttgagt accacggacg gcaggggacg aactgcgcgc cttgaagcgg caaaagcggc
      121 gggaatgagc cccgcgcctc gaccgtcaag cgccgcgcga acaaaaaaac gcgcggttag
      181 cgccgcgcgt tcttgttatc accaaacact gaaacgcact cagaacgcct tcttccaccc
      241 accgccataa gcaatatccg ccagcggcaa acgctgacgc accgactttt cgcgctcgcg
      301 cagcaccggg tcgagcttgt cgacgtctcc gtaatgaccg agcgcaatca tggcgatcac
      361 gtcgacgtcg ttcggtagct tgaatgtagc gcggaacgcg ttcggatcga atccactcat
      421 ctgatgcgcg gcgagaccga gcgcatgcgc ctgcagcacc agcgccatcg ccgcggcacc
      481 cgcatcgtag ggcgcgcaac ggttcacttc gcccttgttg gtgagcgtat gcgtagtcac
      541 cgcgatcagc accggcgccg gcgcattcca gccctgattg aacggcacca gcgtggcgaa
      601 ggcctgcttg aacgagactt catcgacgct gcggtcgaac acgaggaaac gccacggttg
      661 cgcgttatac gaagacggcg cccagcgtgc cgcttcgagc accgcatgca agtgctcccg
      721 gctcaccggc tcgctcgaat aggcgcgcgg actccaacga cctgcaatca gctcgtgaat
      781 ggcaactgcg gtaggggcgg gtttgttggt catgcgcttc tctcggtcga aattcatgat
      841 cgacgggcgg ctgcccgggg tgtcatagca taaccgggct gagccgctcg cgctccaaca
      901 agtgcgcagt tagttctcat gtcagctgca atggcaaacg cccccggatg aggccgtttg
      961 cgctggttca ctgtgttcac cgcgcacagg ggcgctgctt cacaccgctt gtggctccgg
     1021 caccttggcc gggcggttga tccgcagcac gatcacggcg gccaccaggc acagcccgcc
     1081 agaaatcatc gacgctaccg tgtaggtgcc gaggctcgcg cgcaacatgc ccgcgccgag
     1141 cgccgcgaaa gccgccccga gctggtggcc ggcgaccacc cagccgaaca cgaccggcgc
     1201 cgcttccttg ccgtagacat cggttgcgag acgcacggtg ggcggcacgg tggcgatcca
     1261 gtcgagaccg taaaacacgg cgaacaacgg cagcccgaag aaatcgatgc cgaaagcgtg
     1321 cggcagatac atcaacgaca atccgcgcag cccgtaatac cagaacagca gcacgcggct
     1381 attgaagcgg tccgacagcc aacccgacag cgtcgtgccg aacagatcga agatgcccat
     1441 tgcggcgagc agggatgcac cctgcacttc ggtcatcccg tagtcgccgc acatggcgat
     1501 caggtgcgta ccgacgtaac cgttagtgct cgccccgcag ataaagaagc tgaagaacag
     1561 cagccagaaa tcacgcgtct tgctcgccat ggcgagcgta ccgaaggcga tggccagcgg
     1621 attctgcttc gtcacggtgg cagagatcgg cgcgtcggcg ggttcgccga acgggcgcag
     1681 cttcatatcc gccggccgct ccggcaacag aaacgcgacc agcggcaaga cgaccgccgc
     1741 tgcgcctgcg acgacccata ccacctggcg ccagccgtaa tgttcggcga cggccgcaag
     1801 catcggcagg aataccagtt ggccggttgc cgaactggcg gtgagtattc ccatcaccag
     1861 gccacgatgc gtggtaaacc agcgcgtcac cacggtagct gacagcgaca gcgccgcaac
     1921 cccggtcgaa ccgccgacca tcgcgcccca gatcagaacc atctgccacg gatgcgtcat
     1981 cagcgacgac aacgccacac ccgccgccat cgtaccgagc gcgatcagca aggtaggacg
     2041 cacgccaaag cgctgcatcg ccgccgccgc gaatggtccc atcaggccgt agagcgcgat
     2101 gttcacggag atcgcgagcg aaatcgtcgc gcggctccag ccgaattgat gctccagcgg
     2161 caccatcatc acgctcggcg tggcgcgcgt gccggccgcc gcgagcagca ccagaaacac
     2221 caccgccacg gtcagccagc catagtgaaa tcgtccgcca atcagtctcg ctgcccagtt
     2281 catcggttct ccagtcgacg ccttcccgca ggccgcgccg ttcgtattat tacgacttcg
     2341 gtaaaggact tgtgacagat cagtcacaat gagtgttgcg atattagtta ctggtcggta
     2401 acatgtcaag gcatcagttc aattcaggtg gcatgccatg tccgatagtg aaaccctcaa
     2461 accagccggc gcacggcgcg cacgcggcgc gcagacagcc ggcccgcaag cgcagcagca
     2521 tctgttgcgc gccgcggacg aactgtttta tcgcgagggt gtgcgggcgg tcggcgtaga
     2581 cgcggtggtg gagcgcgctg gcgtgaacaa gatgagcttg taccgtcagt tttcgtcgaa
     2641 ggacgaattg gtgatggcgt atctggagcg caaggacgag cagttcttcg gctacgtgga
     2701 gaaaagtttc gcgaagcatc cgggcgagcc cgcgaaacag cttcagcaat atttcgacga
     2761 gctggccgtg cgtgcctcga tcgacgacta ccgcggctgt cctttcgtga atgtctcagt
     2821 ggagtttccc gacgcgaccc atccagcgcg gcaattcgtg tttcacaaca aggcgcggct
     2881 gatggcgcgg cttctggaat tgacgaccgc cacaggcgct gatgatccgg tggccttggc
     2941 caacgcgctg ggtttgatga tcgaaggcgt gtacgcggcc agccagacct atggcccagg
     3001 ctgcgggccg attcttgccg ccccgaaggt agcggcgcag ttgatcgccg cggcgtgcag
     3061 cgggacgcaa gcgggttgag attctcgccg gccgcttgcg gcaaagcacg gatcggtgct
     3121 gcggcgccgg tccgcgccgt tagaatgaca ctttccccat gcgctgcttg ccatgccatt
     3181 gcccgccgac tctcacgatg ccgttattgc cgccactcgt cattggctga cagaagctgt
     3241 gatcgggctc aacttatgtc ctttcgccaa ggccgtgcat gtgaaagggc agattcgcta
     3301 tgccattagc gacgctgtaa atatggaagg agtgttaacc gatcttgaaa ccgagattca
     3361 ggcactcgtc gcggaaaatc ctgaaacggt ggataccacg cttctagttt taccgcaggc
     3421 gctcggcgat tttctcgatt ataacgattg cctgtttttt gccgatcggc ttatcaagca
     3481 gcttcggctg gaaggcatta tccagatcgc cagttttcac cctcattatc agttcgaagg
     3541 cagcgagcct gatgatattg agaattatac gaatcgggcg ccctatccga ttttgcattt
     3601 acttcgcgag aagagtattg aacgggcggt tcaggctttt cccgatgcgg aagatattta
     3661 cgagcggaat caggagactc ttcggcggat cggtcttaga ggctggaatg atttgatgaa
     3721 aatgtagttc gcacggtggt gatgacaaaa ccaccaccgg gagacagatc aggcggcgac
     3781 gaaaaacctt tctttcagct cctcgattcc gagcctctcc atcacttcgt taagcctctc
     3841 cgccggccgc cgtctcggca gatccttgaa ctgggcaata atcagttcat tcttcatcga
     3901 gtgctcccac ccgaccagct cggtcacact cacctgatat ccatgcgctt ccagctgcaa
     3961 gcagcgcagc acgttagtaa tctgactgcc gaattcccgg gtgtgcagcg gatgccgcca
     4021 gatctcagtc aaggcattgc cgagcgattt actcttgttt tgccgtaaaa cccccgccac
     4081 ttcggcctgg caacacggca cgaccacgat gtacttcgca cgcttttcca gcgcaaaccg
     4141 gagcgcgtcg tcagtcgcgg tattgcacgc atgcaatgcc gtcacgatat cgatctggcg
     4201 cggcaaacgc gccgacgtaa tcgattccgc caccgacaaa ttcaggaacg acatcccctt
     4261 gaagcccagc cggttggcca gctcctcgga tttcgtcacg agatcctcgc gcgtttcgat
     4321 gccgtaaatg tgcgacgccc caccaccggc gtcctgaaac tccttgaaga aaaggtcata
     4381 caggataaaa cctaggtatg acttgcccgc gccgtggtcg acgagtgtca ccgcgccctg
     4441 ctggtccttg agctctttca gcagcggctc gatgaactgg aacaggtggt agacctgctt
     4501 cagcttgcgg cggctgtcct ggttcatctt gccgtcgcgc gtcaggatat gaagctcctt
     4561 cagcagctcg acggactggt tcggacggat ttcgtgggtt ttgctggaca tcgtggagac
     4621 cgggaaccgc catcgattgc aacgcgaacg tgcgacagat gacggaaaaa aggaaaagtt
     4681 gctgccagtt tacccaaagc gcagcccgct cacctgagga gccgcgttga ccctgcaaaa
     4741 aatgccgcaa acctggaaca cttcctgctc aaaaaagagc agcacgcggg acggttctga
     4801 cgtggcttga ctgcatcttg cggggcggct cacccgatgg cgcaagccct gtcacgcata
     4861 acaacaggct accggccgtg gcatcgatca cgcgaaccga atgcatgtgg ggaggacggt
     4921 cccggaacac ggaccgaacg ttacgtaacc agcagtgcgc cacgatcgca aacggccgcg
     4981 cgctggataa tagaagaggc agcaaaatgg ataccgtacc caacggcaac gtcgaacaga
     5041 aatttcagga aatgctggcg aagctcaccg ccgcgcccgc gtggtcggaa aagcagcagc
     5101 tcgaacttga gatggcgcgc gacatttcca gcgaaatgct gcggctcgcc gaagtcatgc
     5161 gagatggcag cgtcgacatg gaaacgtgtc tgacgatgct caagtacgcg aaagtgctcg
     5221 acttcgtcat gacgacgctc gcttcgcgtc gcgacatcaa accgcaaacg ctgcgcgtca
     5281 ttttcaagct ggccggcctg aaggtggatg aggcttaccc cgggtaggac gctcaggcaa
     5341 cgccgcgacc cgcgcaggcg gtacgccgcc gctcgcgctt attgattagc cagttcctga
     5401 gcgatcagtc gcagattacg tatcctgcac tggtgccgga gctgacgtgc tccacgaaag
     5461 gacgacgaat cacgcgcgcg gcgcctcgat cggcggcgtg ccgtcatgaa acagcgtctt
     5521 gagctgcgaa cgcagttccg gcgagtccgt atgcttatga accgtaaccg cgtgctgcac
     5581 ggccgcgtcg agcaattcgt tctcgctgtc ggcggatagg gcaacggtgc aattcgtttc
     5641 gctcggaaac tcgcgacagt cgatgtactt gcgagtcatg acaatctcct tcatccaacg
     5701 ttgaaacaaa catccagacc accaacaggc gcgccggcca cgtcgccagc cgcgacgcgg
     5761 ccgcgttcac gacgtcgggc ggactcaaaa cggcgttagt ttgacgcggc gaagacgcga
     5821 actgcgtccg gttgaaggtg tttgaaaagt atagtttgcg tgcggagatt gcaaagaagc
     5881 gagcgcaaac tggacgcgtg cgaaaacgcc cgaacccagc gtgtttcaga cggctcaatc
     5941 ggcatgcgtc accggtttgt gctgcgccgc ccgtgtgtga aaatagcgcc cttcgcctcc
     6001 cgttcgccag atcaggcgcg cgccgcccct ccgcccatcc gcaggaccac atgtcatcct
     6061 ccttcaattc cgcccgtgtc gccgtgatag gcggcggtcc cgccggtctg atggccgctg
     6121 aggcgctggc ccggcacggc gtacaggtcg acgtccatga cgcgatgccg tcagttggcc
     6181 gcaaattcct gatggcgggc aaaggcggca tgaacatcac ccattcggaa ccgctcgagc
     6241 cgtttcttgg ccgctacgga gcccgtcgcg aacagatcga gccattgctc gggatgttcg
     6301 gcccagacgc gctgcgggcc tggttgcagg ggcttggcgt cgacactttc gttggcagtt
     6361 cgggccgcgt ttttccggcc gatatgaaag cggcgccgat gttgcgtgcg tggttgcatc
     6421 ggttgcggga aaccggcgtg cactttcaca tgcgccacaa gtggaccggc tggaaccacg
     6481 atagcggtgt cggccacgcc gctgcgcacg cgttgcgctt cgaaacacct ggcggcgagc
     6541 aaatcgtgac ttgcgatgcc gtggtgttcg cactcggcgg cgcaagctgg ccacgactcg
     6601 gatccgacgc cgcctgggtg ccgctcctga acgcacgcga agtgccggtg acgccgttgc
     6661 ggccggcgaa ctgcggcttc gatgcagact ggagccccta tctgcgcgag cgtttcgccg
     6721 gccagccggt gaaatcggtg gccatcacgc tcaccgatgt agacaataaa gtccacaatc
     6781 gacaaggtga aatacttctg accgaaacgg gcctcgaagg cagtctgatt tacgcgttgt
     6841 cggccgctat ccgggagcga attctggccg acggcgacgt cacgatcacg ctggacctgg
     6901 caccaggctt gacgttggag cgtgtggtgg ccgaggtcac gcgaccgcgc ggctcgcgct
     6961 cgatgtcgac tcacctgcac ggcagagtcg gtattagcgg cgtcaaacta gctttgttgc
     7021 acgagatgct gtcgaaggaa gctttcgccg acgcgaaccg tctggcgcac gcaatcaagg
     7081 cgctgccggt gcggctcacg cgggcccggc ctatcgcaga ggcgatcagc accgctggcg
     7141 gcgttccttt cgaggcactc gatgagcatt tgatgctcaa gcgtttgccc ggcgcgttct
     7201 gcgcgggcga aatgctcgac tgggaagcgc cgaccggcgg ctatctgctc acggcctgct
     7261 ttgccagcgg gctagtggcc ggacgcggcg cattggcgtt tctcgaggcg cgcggtttgc
     7321 ccgtttgaca acgccgcttt tcagcggcgc gtcatcggac cttgctcgcc ttagcgtgcc
     7381 ttcagacgcc agagggatgt cagttcggcc acgcgcgccg tatgcagcgc gtcggtttca
     7441 tcggtcgatt tcggatgtcg cggacggata tcctctcgcc ccactacggt caaccccgcc
     7501 ttttcaatcg cggcgagcag ccactcgcgt gtacgcaagc cgagatgctc ggcgaaatcc
     7561 gacatgatca accagccttc cccgcccgga gacaaatgct ccgccagacc gttcagaaag
     7621 ccgagcaaca tgcggctctc cgaatcgtag atcgcgtatt caatcggcga cgcaggtcgt
     7681 gccgggaccc atggcggatt gcagaccacc agcggcgcac ggccttccgg aaacagatcg
     7741 gcttgcacga cctcgatgtg ttgatcgtag ccaagccgcg tgagattttc gcgcgcacag
     7801 gcgagcgccc gaggatcctg gtcggtcgcg acgattttct tcacgccgcg tctggccagc
     7861 aacgcagcca gcacgcccgt acccgtgccg atatcgaacg ccttgttcaa tgacggcagc
     7921 ggtgtgcgcg caaccagatc cacatattcg ccgcgcactg gcgaaaacac gccgtagtgc
     7981 ggatggatgc ggtccccgag cgcggggatt tccacgccct tcttgcgcca ctcgtgcgca
     8041 ccgatcagcc cgagcaattc acgcagcgac acgaccgagg gttcgtcggt agacggcccg
     8101 taggtctcga tgcaggcctg ctgcacttca ggcgcgcggc gaagcgggat gccataagcg
     8161 gcgtcaagcg gaatcagaat catgccgagc gtacgggcac gctgcgactg cgcctgacga
     8221 tgcagattga acgcgtcgat cggcgtttcg ccctgcttgc gcggtttgcg ttccagccgg
     8281 cgtgtgaccg cctgcagcaa ctgacgggca ttctggaagt caccgttcca caacagcgca
     8341 gtgccttcgc aggcgagacg gtaggcggag tcggcggtgg tgcggtcatc cgcgacgacg
     8401 acgcgcctgg gcggcggcac cgccgcttcg gagcgccaac gcgcggtgcg ggggccgtcg
     8461 gcttcgggcc aggtgatggt tgcggggctg gtcatggtga aaggtggggt ctgtgttgca
     8521 ggagtgacgc ggtgaaacgt cgcgtcttcg ggggcgttgc ccgccggagt gactgatcgt
     8581 gatgccgact gacgcccgaa tagtacctct cccggtcgga ttcgcggcgc ggtatctgct
     8641 ggcggctccc ggcacgagat cgccgccacc gcctttgctg ctgcccattt ggccaaattg
     8701 tggactgaaa agtccataat cgacaaggtg aatgagatct ggtgaaaatg agccgttttt
     8761 tgtctcgccc gagacgccca atgcccgccc caaataaaaa aacgggtttc tgaaattctt
     8821 cagaaacccg ttttcgtact actggtgccg gctgcaggac tcgaacccgc cacctcatga
     8881 ttacaagtca agcgctctac ctgatgagct aagccggcat gaggccgtga ttctacttca
     8941 tttcacgatc ttgaggcgag cccttccgcc accttttgat ccgtcgtcgt caggctgctg
     9001 gccgtcggtc gcggtttccg ctttggcggc agccgcatcg gcggcggttg acgacgattc
     9061 gaccgcgcgc ggcgccgtag cgcgtggtgc cgccggtgcc tcggtctcgt ccgccagttc
     9121 cgcatccgcg cctgaatcct gcgcttcgcc gcccgccgat tcgaccggga acgccatgcc
     9181 ctgcccattt tcgcgcgcgt agatcgcgag aatattggcg atcggcactt cgatcttgtg
     9241 cgacttgccg gagaagcgcg cgctgaactc gatccattca ttgcccatct gcaactggct
     9301 ggtcgcctcg aagctgatgt tcaacacgat ctcgttatcg cgcacgaact gacgcggcac
     9361 gcgcgtctga ttgtcgaccc ggaccgcaat atgcggcgtg tagccgttat ccgtgcacca
     9421 ttcgtacagc gcgcgcagca gataaggctt ggtggaaatc tcttgcatca acagtcctct
     9481 taccgggttg gggcgcgcac ggaaaacaat cgagacgcgg cgccctcacc ccaaagccaa
     9541 actcaacgac gcatcacctt ttccgaaggc gtcagcgctt cgatataagc cgggcggctg
     9601 aaaatgcgct cggcgtactt catcagcggc gcagcgttct tcgacagctc gatgccgtag
     9661 tgatccagac gccacagcaa cggcgcgatc gcgacgtcga gcatcgagaa ttcttcgccg
     9721 agcatgtact tgttcttcag gaagatcggt gcgagctgcg tcaggcgatc gcggatcgcg
     9781 agacgtgcct tttcgtggtt cttctcggca gccttgccct tttcgttttc gagcgtgccg
     9841 acgtgaacga acagttcctt ttcgaagttg agcaggaaca gacgggcacg ggcgcgctga
     9901 accggatcgg ccggcatcag ttgcggatgc gggaagcgct cgtcgatata ctcgttgatg
     9961 atgttcgatt cgtacagaat caggtcccgt tcgacgagaa tcggcacctg accatacgga
    10021 ttcatcacag cgatgtcttc cggcttgtta aacaggtcga cgtcgcggat ctcgaagtcc
    10081 atgccctttt cgaacaacac cagccggcag cgctgggaga acgggcaagt tgtgccggaa
    10141 tacagaacca tcatgtttac gtttcctcaa aaagctgaaa ggccagcgcg cggaaaaacc
    10201 gtccaacagg tttttcctgg cgccggcccc acgccggtga cagcgtgatt atttgatatg
    10261 tttccagtac gcggcgttca gtcgccaggc gaaaaagctc agaatgccga ggaacagcag
    10321 cacccacacg ccaagctgtt tgcgggtttt ctgggtcggt tcggacatcc atgacaggta
    10381 cgacacaaga tcggccacag tagaatcata atctaccggc gacattgtcc ccggcgtgac
    10441 ctgttggaag ccgacaaatt tgtgcaccgt ttcgccggtt ttttcgtcga ctgcgtcacc
    10501 gaacttcgcg gtacgttccc cctgaagctg ccacagcacg tgaggcatgc tcacgttctc
    10561 atacaccaga ttgttccagc cggttggccg cgtattgtcg cgatagaagc tgcgcagata
    10621 cgtgtacagc cagtccttgc cgcgcgcccg tgcttcgacc gacaaatccg gcggcgccgc
    10681 cccgaaccac gctttcgcgt cgtccggacg cattgccacg gtcatggtgt tgccgacttt
    10741 atccgtggtg aacagcaggt tcgcctggat ttcgttcggc gtaatgccaa ggtcggtcag
    10801 ccggttgtag cgcatcaggt tcgcgctgtg acaattcagg caatagttta caaatatttg
    10861 cgcgccgtgc tgcaaagaag cgaaattctc cgcgttatcg ggcgcgcggt cgagagggaa
    10921 attctcgtcc gcgtgcgccg ggccggccag caccgcgagc agtgtcgcgc cgattagcgc
    10981 gcacgtagaa agcagttttt tcatttcgtg ttctcctggc tcgcgattag tggggcttga
    11041 accgcacccg ctcgggcggc tgcttgaacg tgccaagccg cgtccagacc ggcataccga
    11101 ggaaaaacgc gaagtagatc agcgcgcaca cctgcgcaat caccgtcgaa gccggcgacg
    11161 gcgggttggt gccgaggaag cccaacgtca ggaaagccag cacgaagatc cggtagaaca
    11221 ccttgtgaaa aaacggccgg taacgaatcg acttcaccgg cgagcggtcg agccacggca
    11281 ggaagaacag cgagatcacc gcgccgccca tcaccaccac accccagaac ttcgattcgg
    11341 tgaacgccat ggcgagcacc accagcacgg cgagcaccgg caaaccgagc ttccacttgc
    11401 cgcgcgcgcg caccagcgcg aacaggccga gcagcgcgac gatgatcatc aacacgatct
    11461 tgaacggatc agtggtggcg cgcagcattg catagaaggc ggtgaagtac cacaccggcg
    11521 caatttcctg cggcgtttga agcggattgg cggggatgaa gttattcgct tcgaggaaat
    11581 acccgcccat ctccggcgcg aagaagatga tcaccgcgaa gatcagcagg aacaccgtca
    11641 cgcccatgaa atcgtgcacc gagtagtacg ggtggaacgg aatgccgtcg agcggaaccc
    11701 cgtccgggcc cttattcg
//
LOCUS       DATASH010000663         2815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1890407,
            whole genome shotgun sequence.
ACCESSION   DATASH010000663 DATASH010000000
VERSION     DATASH010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1890407"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VHV99_17875"
     CDS             complement(<1..495)
                     /locus_tag="VHV99_17875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX3637315.1"
                     /translation="MVKIDPDRLLTDLKQLRSFGATGPGVVRLALSPVDLASRRWLAG
                     RMAQAGLDAVIDGVGTVFGRSRKSGPALVVGSHTDTQPTGGWLDGAMGVIYGLEIARA
                     LGENEATRHLAVDVASWIDEEGTFSGLLGSRSFVGDPVEESIRSATNREGQRLHDVLE
                     AAGLA"
     gene            complement(625..2217)
                     /locus_tag="VHV99_17880"
     CDS             complement(625..2217)
                     /locus_tag="VHV99_17880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3637316.1"
                     /translation="MRLAVRNAALMLLTCLALGASACVQAGSSSGCDEECNSRGQLLD
                     NRVTLRLSAAAFSTLLAANASGRQLEQIAGVPACSVEIVTFRYRTIGGAGEPTTASGA
                     LMIPGGRSAACSGPRPLMLYAHGTSAYRNYNIADITRTDPGNGAGAGEGLTVAAMYAA
                     QGYIVVATNYAGYAGSDLAYHPYLNAGQQSADVIDSLRAARAVLNETKPPGRTRENGK
                     LFVTGYSQGGFVALATHRALQAAGIKVTASAPGSGPYALAATADALIEGEVNLGSTLF
                     TTLIVTGYQHAYQTIYRKPDEFYEAAYAPDIENLLPSLQPLDTLIAQGKLPSTHLFDS
                     VPPAPRFASMTLPASPPLAPPALTPLFAAGFGNANLVRNAYRLGLLEDAAAHPDGAFP
                     NSTVAMTPAENPMHPLRQAFKRNDLRNWLPRAPVLLCGGGLDPTVFFFNARIEQAYWQ
                     NAKVPAGLTSVLDVDTPASGPDDPYAPIKQGFASAKATLANRAVAGGATDGGASAVLR
                     AYHGELVASFCMLAARAFFANF"
     gene            complement(2231..2323)
                     /locus_tag="VHV99_17885"
                     /pseudo
     CDS             complement(2231..2323)
                     /locus_tag="VHV99_17885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007730759.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(2396..>2815)
                     /locus_tag="VHV99_17890"
     CDS             complement(2396..>2815)
                     /locus_tag="VHV99_17890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX3637317.1"
                     /translation="RGAVQYLKQHSTNVGVTGYCMGGALTLLALCNIPELSAGVVWYG
                     FPPLEYIDASKIKVPVLGHWATQDQFFAAETVDALEAKLTAANVGIEFHRYLAHHAFA
                     NETAVGPGRIAGTQFDSVWSQQAWDRTLTFFGRTLWK"
BASE COUNT          520 a          892 c          915 g          488 t
ORIGIN      
        1 ggcaaggccg gcggcttcga gcacgtcgtg aaggcgctgc ccttcccggt tcgtggcgct
       61 tcggatcgac tcctcgacgg gatcgcccac aaagctgcgg ctgccaagca aaccggaaaa
      121 ggtgccttct tcatcgatcc aggacgccac atccacggca aggtggcgcg tcgcttcgtt
      181 ctcgcccagc gcgcgagcga tctccaaccc gtagatgacg cccatcgcac cgtcgagcca
      241 accgcccgtg ggctgcgtat cggtatgaga gccgacgacg agcgcgggac cgcttttgcg
      301 cgagcggccg aacaccgtcc ccacgccgtc gataacggca tcgagtcccg cctgcgccat
      361 gcgtccggcc agccaccgac gcgacgcgag gtccaccggc gagagcgcga ggcgcacgac
      421 gccagggccc gtggcgccaa agctgcgcag ctgtttgaga tcggtcagca accggtccgg
      481 atcgatcttc accacgttca gatttccttt gatgttggcc tatgccgccg aggggtttgc
      541 cgacatgcat agagaagatt acacggcttg acgctgggcg attctatcga tcgctcgcgc
      601 gtgcagccag cccgcctgtg acgctcagaa attagcgaag aatgcccgcg cggcgagcat
      661 gcagaacgac gcgaccagtt cgccgtgata ggcccgaagc acggctgaag cgccgccgtc
      721 ggttgctccg cctgccacgg cccgattcgc gagcgtcgcc ttcgcacttg cgaagccctg
      781 cttgatgggt gcgtacgggt cgtcaggccc gctggctggc gtgtccacat ccagcacgga
      841 tgtaagccct gccggcactt tggcgttctg ccagtaggcc tgctcgatac gtgcattgaa
      901 gaaaaacacg gtgggatcca agccgccgcc gcacaacaga accggcgcac gcggcagcca
      961 gttgcgtaag tcattgcgct tgaacgcctg acgcagcgga tgcatgggat tttccgccgg
     1021 cgtcatggca accgtgctgt tgggaaacgc gccgtcggga tgggccgcgg cgtcttccag
     1081 aagacccagg cgataggcgt tgcgtacgag attggcgttg ccgaatcccg cagcaaacaa
     1141 aggtgtcagc gcgggcggtg cgagaggtgg cgatgccggc agcgtcatcg acgcgaatcg
     1201 cggcgcggga ggcacgctgt cgaagagatg cgtcgagggc aatttcccct gcgcgatcag
     1261 tgtatcgagg ggctgcaggc tgggcagaag attctcgatg tcgggggcat aggccgcttc
     1321 atagaactcg tccggcttgc gatagatggt ctgataagca tgctggtagc ccgtcacgat
     1381 caaggtggtg aacagcgtcg aacccagatt cacttcgcct tcgatgagcg cgtccgcggt
     1441 ggcggcgagc gcatagggac ccgatcctgg cgcggatgcg gtgaccttga tgccggccgc
     1501 ttgcaaagca cgatgcgtcg ccaatgctac gaagccgcct tgcgaatagc cagtgacgaa
     1561 gagcttgccg ttctcgcgcg tcctgcctgg cggcttcgtt tcattcagga cggcgcgcgc
     1621 cgcccgcagc gagtcgatca catcggcaga ttgctggccg gcgttcaggt aggggtggta
     1681 cgcgagatcg gagccggcgt agccggcata gttggtcgcc accacgatat acccttgggc
     1741 cgcgtacatg gcggcaacgg tcagcccctc cccggcgccg gcgccgttgc ccggatccgt
     1801 tcgtgtgata tcggcgatgt tgtagttacg gtatgcactc gtaccgtgcg cgtagagcat
     1861 cagcgggcgc ggcccgctgc acgcagccga ccgtccgccc ggaatcatca atgcaccgct
     1921 cgcggtggtg ggctcgcccg cgccgccgat ggtccggtag cggaaggtca cgatctccac
     1981 actgcacgcg ggcaccccgg caatctgctc gagttgccgg ccggatgcgt tcgctgcaag
     2041 caatgtcgag aatgccgcgg cactaagccg caacgtgacg cgattatcga gaagctggcc
     2101 gcgagagtta cattcctcat cgcaaccgga gctggagccg gcttgcacac aagcactggc
     2161 gcccaacgcc aaacatgtca gcagcatgag ggccgcattt cgcaccgcta gacgcatgtg
     2221 aggctccgtg caggaacgca aggtcgagct tgccgcgggc gagtgcatcc gcgcggttcg
     2281 gtgaatagtg gctcgacacc gtcacgtcga tgttcggcag ttcgtgggca ttcagccagc
     2341 ggcgcgcacg cccgccgctg gcattcacct caagacgcag gttgccgctt tcccgctact
     2401 tccaaagcgt gcggccgaag aaagtcagcg tgcggtccca ggcctgctgc gaccagaccg
     2461 aatcgaactg ggtcccggcg atacggcccg gcccgactgc cgtttcgttc gcgaacgcat
     2521 ggtgcgcgag atagcgatga aactcgatgc cgacattggc cgcggtgagc ttggcctcca
     2581 gggcgtcgac cgtttcagcc gcaaagaatt ggtcttgcgt cgcccagtgg ccgagaaccg
     2641 gtactttgat tttcgaggcg tcgatgtatt cgagcggcgg gaagccgtac cagaccacac
     2701 cggccgacaa ttcagggata ttgcatagcg cgagcaaggt gagcgcgcca cccatgcaat
     2761 agccagtcac gccgacgttc gtcgaatgct gcttcagata ctggaccgcg ccccg
//
LOCUS       DATASH010000664         5867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4659859,
            whole genome shotgun sequence.
ACCESSION   DATASH010000664 DATASH010000000
VERSION     DATASH010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5867
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4659859"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="VHV99_17895"
     CDS             complement(<1..921)
                     /locus_tag="VHV99_17895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011851010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX3637318.1"
                     /translation="MDLNFTPEEEAFRTDVQRFLRDRLPQRLADKVHGGRRLTRADMA
                     EWHAILNAQGWLANHWPKEYGGPGWNAVQKFIFENECALAGAPRVVPFGVNMLGPVLI
                     KYGNDAQKRHWLPRILDGSDWWCQGYSEPGAGSDLASVKTTAVRGSDAQGEHYVVNGQ
                     KTWTTLGHYANMIFCLVRSATDVRKQEGISFLLIDMNTPGVDVRPIITLDGEHEVNEV
                     FFTDVRVPVENLVGEENKGWTYAKYLLTYERTNIAGVGGSMAAFDWLKHVAGAQKKAG
                     KPLAVDPYFAARLAKLEIDLENMKTTNLRVL"
     gene            complement(1040..3160)
                     /locus_tag="VHV99_17900"
     CDS             complement(1040..3160)
                     /locus_tag="VHV99_17900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018435263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX3637319.1"
                     /translation="MTQNASADVVTRELRGKVLLVTIDHPPVNALSADVRRGLLAAIE
                     AADADPAVEAVLIVGAGRNFIAGADIREFGKPPVPPSLPDVCNRIEACTKPVVAAIHG
                     AALGGGLEVALAAHYRLAVNGAKLGLPEVQLGLLPGAGGTQRTPRLIGAQSALDLILS
                     GRHAGAKEALTLGLIDRLGSSDDILAEGLAYVHGLLAAHAPVRRTRDAAALSDRAASL
                     AAVATARAETAKKSRGLFSPLKIVDAVEAAIEQPFEDGLRLERKLFLECIDSPQRAGL
                     IHAFFAEREVLKAPETRDAKPRPLNQIGVVGGGTMGAGIAVAVFDAGLPVTMIERDEA
                     SLARGRAHIDKVYDGLITKGRMSAEKKADVMARWHGSTSYDALAEADLVIEAVFEDLA
                     VKKAVFAELDRVCKAGAVLATNTSYLDIDAIAASISRPADVIGLHFFSPANIMKLLEV
                     VVPKQVSADVVATAFELAKKLRKTPVRAGVCDGFIGNRVLAVYRSAADAMMEDGASPY
                     QIDAAVRAFGFPMGPFQVVDLAGGDIGWAARKRRAPTRNPAARYVQIADRLCERGWFG
                     QKTGRGFYLYPEGSRSGTPDPEVEAIIDAERERAGITPRTFTDEEIMRRYMAAMINEG
                     ANVVHEGIALRPLDVDVTFLYGYGFPRYRGGPMKYADTVGLATVLADIREFAQEDPLF
                     WQASPLLVELVERGADFASLNQIA"
     gene            3318..4226
                     /locus_tag="VHV99_17905"
     CDS             3318..4226
                     /locus_tag="VHV99_17905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3637320.1"
                     /translation="MDVNSLTLLVDILDAGNLSEAARRLKMSRANVSYHLNQLERSVG
                     LQLVRRTTRRVEATEIGLQLYEHGRTIQNALLAARESVTTLGQSLQGRVRLSVPSGYG
                     QLVMSDWLIAFKRLYPGIVLDVMFENRVEDLMRDEVDIAVRVMSEPPQNLVARDMGPV
                     RYVACASPDFAASRGMPTRLDDLRTAPLITAAVVGRQLRVAAYLRDERHEVLLEPTII
                     SENFLFLRQAVLAGLGVGLVPDYVVQDDLRRGEVVTALDEWRLSIFGTNMYMLYMPNR
                     HHTRAAASFIEFMLEQARGSGRGVAA"
     gene            4314..4502
                     /locus_tag="VHV99_17910"
     CDS             4314..4502
                     /locus_tag="VHV99_17910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637321.1"
                     /translation="MKWAIACAALLAIVIGVVMVSRLPGEAAIRLAGYAATAAFAALV
                     GFLIRWRAHRARRNRRAG"
     gene            complement(4583..5428)
                     /locus_tag="VHV99_17915"
     CDS             complement(4583..5428)
                     /locus_tag="VHV99_17915"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX3637322.1"
                     /translation="MPVVRIGKDKQHAIYLLRGEANTWLGIRSKAHALSRYSVIEPET
                     LQGLILADSTSGDSHYFDVLLPKRANWKQALFDRANRIKGFDIHFPYPPSGETGDEFI
                     CPDSFWTECATNPDRLDQEESHFRSFTINFLQDIVYPGCVIYDPACSTGSFSGYLKAS
                     LPQCIVLASDASLSMTQAAKNQIDRAFVCDALRPALRDNSCDVLICRFLNHEVMTTTL
                     ANKILSKMVPLIKHNGKIIIFGHTPILIDIPRISQKYGMTLTSSLAHSEIPNGLFQYY
                     IVGFH"
     gene            complement(5438..>5867)
                     /locus_tag="VHV99_17920"
     CDS             complement(5438..>5867)
                     /locus_tag="VHV99_17920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637323.1"
                     /translation="VHVMGAGCSIYSSHAASRAIKELVQHHRIAEGVEWVRDEWRRHM
                     LHLSPIPRLQQCLMADLDSQQGIKMSEVSLPADRSILPLAAQLALLKDRCRTAGLPIW
                     SKELHTAELGISLACAAMPNMERFSIVSLGNYVVPTYNYI"
BASE COUNT         1060 a         1725 c         1886 g         1196 t
ORIGIN      
        1 cagcacgcgc aggttcgtcg tcttcatatt ttcgagatcg atctcgagct tggcgaggcg
       61 cgcggcgaaa tagggatcga ccgccagcgg cttgcccgcc ttcttctgcg cgccggcgac
      121 atgtttgagc cagtcgaacg ccgccatcga tccgccgacg ccggcgatgt tggtgcgctc
      181 gtaggtcagc agatatttgg cgtaggtcca gcctttgttc tcttcgccca cgagattctc
      241 aacgggcacg cgcacgtccg taaagaacac ttcgttgact tcgtgctcgc cgtcgagcgt
      301 gatgatcggc cgcacgtcga cgcccggcgt gttcatgtcg atcagcagga agctgatgcc
      361 ttcctgcttg cgcacgtcgg ttgcgctgcg cacgaggcag aagatcatgt tcgcgtagtg
      421 accgagcgtg gtccacgttt tctgaccgtt gacgacgtaa tgctcgcctt gcgcgtcgct
      481 gccgcgtaca gcagtggtct tgaccgatgc gaggtccgag cccgcgcccg gttccgagta
      541 accctggcac caccagtccg agccatcgag aatgcgcggc aaccagtggc gcttttgcgc
      601 gtcgttgccg tatttgatca acaccgggcc gagcatgttg acgccgaacg gcacgacgcg
      661 cggtgccccc gccaaggcac attcgttctc gaagatgaat ttctgcacgg cgttccagcc
      721 ggggccgccg tactccttcg gccaatgatt ggcgagccag ccttgcgcgt tgagaatggc
      781 gtgccactca gccatgtcgg cgcgcgtaag gcggcggccg ccgtgtacct tgtcggcgag
      841 acgctgtggg agcctgtcac gcaaaaagcg ctgcacgtcg gtgcggaaag cttcttcttc
      901 aggcgtgaag ttcagatcca tggcgggttc tttcctggag gtttattgga ccggcatacg
      961 ggtgtaccga tcgatgcacg gcataccggc gggcaggcag ccgcccgccg gtcgggaatg
     1021 cgccagatga cgtgccgtct caagcgatct gattgaggct tgcgaaatcg gcgccgcgct
     1081 caaccagttc cacgagcagg ggcgacgctt gccagaacag cgggtcttcc tgggcgaact
     1141 cgcggatgtc cgcgagaacc gtggcgaggc ccaccgtatc ggcatatttc atcggaccgc
     1201 cgcgatagcg cgggaagccg tagccgtaaa ggaacgtgac gtcgacgtcg agcggccgca
     1261 gcgcgatgcc ttcgtgcacg acgttggcgc cttcgttgat catcgcggcc atgtagcggc
     1321 gcatgatttc ttcgtcggtg aacgtgcgcg gcgtgatgcc ggcgcgttcg cgctcggcgt
     1381 cgatgatggc ctcgacttcc gggtccggcg tgccgctgcg cgaaccttcg gggtacagat
     1441 agaaaccacg gccggtcttc tggccgaacc agccgcgttc gcacaggcgg tcggcgatct
     1501 gcacgtagcg tgcggcggga ttgcgcgtgg gcgcgcgccg tttgcgcgca gcccagccga
     1561 tgtcgccgcc cgccagatcg accacctgga acggccccat cgggaagccg aacgcacgca
     1621 cggccgcatc gatctgatag ggcgacgcac cgtcttccat catcgcgtcc gccgcgctgc
     1681 gatagaccgc cagcacgcga ttgccgatga agccgtcgca cacgcccgcg cgtaccggcg
     1741 tcttgcgcag cttcttcgcg agttcgaacg cggtggcgac cacgtccgcg ctgacctgct
     1801 tcggcaccac cacttccagc agcttcatga tgttggcggg ggagaagaag tgcaggccga
     1861 ttacatcggc ggggcgcgag atgctggccg caattgcgtc gatgtcgaga tacgacgtgt
     1921 tggtggcgag caccgcgccg gccttgcaga cgcgatcgag ctcggcgaac acggcttttt
     1981 tcaccgccag gtcttcgaag acggcttcaa tcaccagatc ggcttcggcg agcgcgtcgt
     2041 acgaagtact gccgtgccag cgtgccatta cgtcggcttt tttctccgcg ctcatgcggc
     2101 ctttggtgat caggccgtcg tagaccttgt cgatgtgcgc ccggccgcgc gcgagcgacg
     2161 cctcgtcgcg ttcgatcatc gtcaccggca ggcctgcatc gaaaacggcg acggcgatac
     2221 ccgcgcccat cgtgccgccg cccaccacgc cgatctggtt cagcgggcgt ggttttgcat
     2281 cgcgcgtttc cggcgccttc agcacttcac gctcggcaaa gaacgcgtga atcaagccgg
     2341 cgcgctgcgg gctgtcgatg cattccagga acaatttgcg ttcgagccgc aggccgtctt
     2401 caaagggttg ttctatcgcc gcctcgacgg catcgacgat tttgagcggc gagaacaggc
     2461 cgcgcgattt cttcgccgtt tcggcgcgtg ccgtggcgac ggcggcgagg ctcgcggcgc
     2521 ggtcgctgag cgccgcagcg tcgcgcgtgc gccgcaccgg agcgtgagcg gccagcagtc
     2581 cgtgcacata ggcgaggcct tcagcgagga tgtcgtcgct gctgcccagc cggtcgatga
     2641 ggccgagcgt gagcgcttcc ttcgcgccgg cgtgacggcc gctcaggatc aggtcgaggg
     2701 cggattgcgc gccgatcagg cgcggcgtac gctgcgtgcc gcctgcgccc ggcagcaggc
     2761 cgagctgcac ttcgggcaaa ccgagcttcg cgccattcac ggcgagccgg tagtgcgccg
     2821 cgagcgccac ttccagaccg ccgccgagcg ccgcgccgtg aatcgcggcc acgacgggct
     2881 tcgtacaggc ttcgatgcgg ttacagacgt ccggcagcga cggcggcacc ggcggcttgc
     2941 cgaactcgcg aatgtccgcc cccgcgatga agttgcgccc ggcgccgacg atcagtacgg
     3001 cctccacggc tgggtcggca tccgcggctt cgatggcggc gagcaggccg cgccgcacgt
     3061 cggctgacaa ggcgttgacc ggcgggtggt cgatcgtcac cagcaggact ttgccgcgca
     3121 attcgcgcgt cacaacgtcg gcggaagcgt tctgggtcat cgtgtcgtct cctgactgtc
     3181 tgttaccggg ccggatcgag gtggattggg acataccggc tctggcgagt tctccgattg
     3241 tcggttggtc aaggtttatt gacaattgca tagacggttg acagactgtc aagattattt
     3301 tgacaaaggc atgcgtcatg gacgtcaatt cactgacctt gctggtcgat attctcgatg
     3361 cgggcaacct gagcgaagcc gcgcgccggc tgaaaatgag ccgcgcgaat gtgagctatc
     3421 acctgaatca actggagcgc tcggtcgggc tgcaactggt caggcgtacc acgcgccgcg
     3481 tcgaagcgac tgaaatcggt ctgcagctat atgagcatgg ccgcacgatc cagaacgcgc
     3541 tgctggccgc gcgcgagtcg gtgaccacgc tcgggcagag cttgcagggg cgggtgaggt
     3601 tgagcgtgcc gagcggctat gggcaactgg tgatgtcgga ttggctgatc gcattcaagc
     3661 gtctttatcc gggtatcgtg ctggatgtga tgttcgagaa tcgcgtcgag gatctgatgc
     3721 gtgacgaggt cgacatcgcg gtgcgggtga tgtcggagcc gccgcaaaat ctcgtcgcgc
     3781 gcgacatggg gccggtgcga tatgtggcct gtgcttcgcc ggattttgcc gcaagccgcg
     3841 gcatgccgac gcggctcgat gatctgcgta cggcgccgtt gattacggcg gcggtggtcg
     3901 gcaggcagtt gcgcgtggcg gcttatctgc gcgacgagcg ccacgaagtg ctgctggagc
     3961 ccacgatcat ctccgagaac tttctgttcc tgcgccaggc ggtgctggcc ggcctgggcg
     4021 tcggtctggt gcctgactac gtggtacagg acgacctgcg ccgtggggag gtggtgacgg
     4081 cgctcgatga atggcgtcta agcatcttcg gcacgaacat gtacatgctc tatatgccga
     4141 atcgtcatca cacgcgggcc gccgcgagtt ttatcgagtt catgctggag caggcacgcg
     4201 ggagtggcag aggggttgcc gcttgagcgg caagtcgttg gcggtatgcc tggtaaaacg
     4261 gcggcatagc cgtgcgcgcc cgggcggcac attaaacctc aggggagacg gagatgaaat
     4321 gggcaatcgc atgtgcggct ctgcttgcaa tcgtcattgg cgttgtgatg gtctcgcgtc
     4381 tgccgggcga agcggccatc cgcctcgccg gctatgcggc gaccgcggcg ttcgccgcac
     4441 tggtcggttt cctgatccgc tggcgcgcac atcgcgcgcg acgaaacagg cgcgccggat
     4501 gaacaggcgc gccggttact cagctgaccc aggtgaaagc tctgcagcgc ccagtccttt
     4561 tttcggacgt gcgcctgcaa actcagtgga acccgacgat atagtattgg aataggccat
     4621 tcggaatctc cgagtgtgca aggcttgatg tcagtgtcat tccatatttc tgactgatcc
     4681 tggggatgtc aattaaaatt ggagtgtgcc caaaaattat aatttttccg ttgtgcttta
     4741 tcagtggcac catttttgaa agaattttat tcgccaaagt ggttgtcatc acttcatggt
     4801 tcaggaagcg acaaatcagg acgtcgcacg aattatcccg caatgcggga cgcagtgcat
     4861 cgcagacaaa tgcgcgatct atttggtttt tcgctgcctg agtcatcgat agcgaggcgt
     4921 ccgatgccag aactatgcat tggggtagcg atgcctttag atagccagaa aacgagccag
     4981 ttgaacaggc agggtcatag atgacgcagc cgggataaac gatatcttgc agaaagttga
     5041 tagtaaaaga gcggaagtgc gattcctcct gatccagacg gtctgggttt gtagcgcatt
     5101 ctgtccagaa ggaatccggg catataaatt catcacccgt ctctcctgaa ggggggtagg
     5161 ggaagtgaat gtcaaaccct tttatccggt tagcgcgatc aaataatgcc tgcttccagt
     5221 tggcgcgttt cggcagcagg acatcaaaat aatgagagtc accggaggtt gagtcggcca
     5281 gtattaaccc ctgcagggtc tcgggttcaa tgacgctata ccggctcagc gcatgcgctt
     5341 ttgatctgat gccgagccag gtattggctt cgccccttag cagatagatt gcatgctgtt
     5401 tgtcttttcc tatcctgact accggcatca gttcgactca gatgtagtta taggttggaa
     5461 caacgtagtt gccgagcgat acgatagaga atctctccat attcggcatc gcggcacagg
     5521 ccaggctgat gcctagctca gcggtatgca gttctttgga ccagatgggc aatcctgcgg
     5581 tcctgcacct gtctttaagt aaagcaagtt gtgcggcgag cggcagaatg gatcgatccg
     5641 ccgggaggga cacctcgctc attttgattc cctgttgtga atcgaggtct gccatcaaac
     5701 attgctgaag ccgggggatc ggacttaaat gcagcatatg ccgccgccac tcgtcgcgaa
     5761 cccactcgac accttcggcg atgcggtggt gttgaaccaa ttccttgatc gcccgcgacg
     5821 ccgcgtgact ggaatagatt gagcaacccg ctcccatcac gtgaaca
//
LOCUS       DATASH010000665         1857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1296492,
            whole genome shotgun sequence.
ACCESSION   DATASH010000665 DATASH010000000
VERSION     DATASH010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1296492"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="VHV99_17925"
     CDS             complement(<1..484)
                     /locus_tag="VHV99_17925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein FimV"
                     /protein_id="HEX3637324.1"
                     /translation="MNLRLSSLRAMFMYQGTGHSTVATALALALAGVSVGAGNAVAAP
                     GDATSAPAGASVSYAAGSQYTVKPGQSLNDVAIAVTQSRDQATLVRASRALFDANPSG
                     FMKHDPSRLRVGAVLTIPALDASGALVASAPVAGSASTAPSTATAAAASAPKAPNTAT
                     A"
     gene            complement(624..1745)
                     /gene="asd"
                     /locus_tag="VHV99_17930"
     CDS             complement(624..1745)
                     /gene="asd"
                     /locus_tag="VHV99_17930"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012338485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX3637325.1"
                     /translation="MNVGLVGWRGMVGSVLMQRMQQEGDFDLIEPVFFSTSNAGGNAP
                     SFAKNETKLKDATSIEDLKKCEAIISCQGGDYTNEVFPKLRAAGWNGYWIDAASSLRM
                     KDDAVIILDPVNLDVIKNALVKGQKNFIGGNCTVSLMLMALGGLFRENLVDWMTAMTY
                     QAASGAGAQNMRELLQQMGTLYGAAKEDLADPSSAILDIDRRVLSAMNSDRMPTDNFG
                     VPLAGSLIPWIDKDLGNGMSKEEWKGGAETNKILGKPAMGTPGSIPVDGLCVRIGAMR
                     CHSQALTIKLNKDVPLDEVNSILASGNDWVKVVPNEREASMRDLSPAVVTGTLTVPVG
                     RVRKLAMGGEYLSAFTVGDQLLWGAAEPLRRMLRIVLDK"
BASE COUNT          343 a          604 c          550 g          360 t
ORIGIN      
        1 gcgcggtcgc cgtgttcggt gccttcggtg cggaagcagc cgcagccgtg gcggtcgatg
       61 gcgcagtaga cgcggaccca gcaaccggcg cggaagccac cagagctccg gacgcatcca
      121 gtgccggaat cgtcaacacc gcgcctacgc gcaaccggct cgggtcatgc ttcatgaacc
      181 cgctcggatt cgcatcgaac agcgcgcgcg aagcgcgtac gagcgtcgcc tgatcgcgcg
      241 actgcgtgac ggcaatcgcc acgtcgttca acgactgacc aggcttcacc gtatattgac
      301 tgccggcggc ataagaaacg gaagcgcccg ccggcgcgct tgtggcgtcg cccggcgctg
      361 ccactgcgtt acctgcgccg acactgacgc cagccagcgc caaagccaga gcggtcgcga
      421 ccgtcgagtg gcccgtacct tgatacatga acatagcccg aagggaagag agtcgaaggt
      481 tcatcgggac tcctggcgcg tcagcgcgca gccctttttt gcggttgaga agagacaaga
      541 taatcggagc acacaatgaa aaaagcgccg cgcaagcgcg acgcttcttg agttttctac
      601 gcgtcgtgag cgcgtagttt actttacttg tcgagcacaa tgcgaagcat gcgacgcagc
      661 ggttcagccg cgccccacag cagctgatcg cccaccgtga aggccgacag atattccccg
      721 cccattgcca gtttgcgcac gcggccgacc ggcacagtca gcgtgcccgt caccacggcg
      781 ggggacagat cgcgcatcga cgcttcacgt tcgttcggca cgaccttgac ccagtcgttg
      841 cccgacgcga ggatgctgtt cacttcgtcc agcgggacat ccttattcag cttgatggtc
      901 agcgcctgcg agtgacaccg cattgcgccg atccgcacgc acagcccatc taccggaatc
      961 gaacccggcg tgcccatggc cggcttgccg agaatcttgt tggtttccgc gccgcccttc
     1021 cactcttcct tcgacatgcc gttgccgaga tccttgtcga tccacggaat cagcgagccg
     1081 gcgagcggca cgccgaaatt gtcggtcggc atacggtcgc tgttcattgc ggagagcacg
     1141 cggcggtcga tgtcgagaat cgccgacgac ggatccgcca ggtcttcctt ggccgcgccg
     1201 tacagcgtgc ccatttgttg cagcagttcg cgcatgtttt gcgcgcccgc gcccgaagcg
     1261 gcctgatacg tcatggccgt catccagtcg acgaggtttt cgcggaacag accgcccagc
     1321 gccatcagca tcaggctgac cgtgcagttg ccgccgatga aattcttctg gcccttgacc
     1381 agcgcgttct tgatcacgtc gaggttgacc ggatcgagaa tgatgaccgc gtcatccttc
     1441 atgcgcagcg acgaagccgc gtcgatccag taaccgttcc accccgccgc gcgcagcttc
     1501 gggaacactt cgttcgtgta gtcgccgccc tggcaggaga tgatcgcttc gcacttcttc
     1561 aggtcttcga tgcttgtcgc atctttgagc ttggtctcgt ttttggcgaa cgacggcgcg
     1621 ttgccgcccg cgttgctggt gctgaaaaac accggttcga tcaagtcgaa atcgccttcc
     1681 tgttgcatgc gttgcatcag gacgctgccg accatgccgc gccaacctac gagacctacg
     1741 ttcatgactt catacccttc gaatggagac ttccccgcat ctttgcccgc agtgaccgcg
     1801 cggggaagat gagcgggcac gacggacgat cagcgtttcg tgatcgtttt cggggta
//
LOCUS       DATASH010000666         3183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5055646,
            whole genome shotgun sequence.
ACCESSION   DATASH010000666 DATASH010000000
VERSION     DATASH010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5055646"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..832)
                     /locus_tag="VHV99_17935"
     CDS             complement(116..832)
                     /locus_tag="VHV99_17935"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HEX3637326.1"
                     /translation="MNNPYPAPTFTGPRLQAAIIDLDGTMIDTADDFTAGLNGMLAQL
                     DATETSREEVIGYIGKGSENLIRSVLAQRFETDHAQDRFDEALAIYQVEYAKINGVHT
                     RVYPEVEAGLSAMRDAGLKLACVTNKPHRFAVELLEQYGLSQYFSVVLGGDSLPQKKP
                     DPLPMLTAAARLGVAPQATVAIGDSENDALAGRAAGMATLTLPYGYNHGRAIQEIKSD
                     GIVASLLDAAKAIAAHHSTT"
     gene            complement(859..1542)
                     /gene="rpe"
                     /locus_tag="VHV99_17940"
     CDS             complement(859..1542)
                     /gene="rpe"
                     /locus_tag="VHV99_17940"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX3637327.1"
                     /translation="MTQFRIAPSILSADFSRLGEEVRNVVAAGADWIHFDVMDNHYVP
                     NLTIGPLVCEAIRPHVNVPIDVHLMVRPVDRIVPDFAKAGANLISFHPEGSDHIDRTL
                     SLIRDHGCKAGLVFNPATSLSYLDYVMDKVDLVLIMSVNPGFGGQSFIPEALVKLREA
                     RKRINAYKEKTGREIHLEVDGGVKVDNIAEIAAAGADTFVAGSAIFGAPDHKAVIDKM
                     RAALANIEH"
     gene            1818..2192
                     /gene="apaG"
                     /locus_tag="VHV99_17945"
     CDS             1818..2192
                     /gene="apaG"
                     /locus_tag="VHV99_17945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HEX3637328.1"
                     /translation="MSQYEFSVSAQVQYLPEESDPERRQYAFAYTLTIRNSGQVPAQL
                     IARHWVITDSENNVQEVKGLGVVGHQPLLKPGEHFEYTSWAVIATPVGTMRGDYFCVA
                     EDGERFDAPVPEFMLRMPRTLH"
     gene            complement(2239..2400)
                     /locus_tag="VHV99_17950"
     CDS             complement(2239..2400)
                     /locus_tag="VHV99_17950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637329.1"
                     /translation="MSDLFDEYPMLIFALESLFALVLLVFIVVWTSSGKKKAARAASN
                     EQQKQQKSQ"
     gene            2429..>3183
                     /locus_tag="VHV99_17955"
     CDS             2429..>3183
                     /locus_tag="VHV99_17955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006052787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HEX3637330.1"
                     /translation="MRFVRTAGAWLGALSVAVLLASCGGGGAVRPSVSPPTGAAIIPG
                     QIAATRLTAVAWQQVPGWQDDSLIGATAALRQNCLRLARQPNWARACAAASQIDDLDV
                     TGARAFFEAYFTPFQLANNDGTLDGLVTGYYEPLLRGSRTRHGVYQTALYRWPSGYRA
                     GAPLPPRAQLEREGVLNGNELVWVDDPIEAFFLQVQGSGRIVMEDGSVMRLGFGGTNN
                     QPYKSIGRWLLDRGELTPAQATMQGIKAWARANP"
BASE COUNT          586 a          933 c         1029 g          635 t
ORIGIN      
        1 agcttcagcg tacctcaggc gaggttagcg cgaccagcga cgccagggcc aggctccccg
       61 gtcgatgcta atcagactcc gttttttatt cagaaacatg aggatgaagg acttttcaag
      121 tcgtggaatg gtgcgctgcg atagccttgg cggcgtcgag cagcgaggca actataccat
      181 cggattttat ttcttgtata gctcggccgt ggttgtaacc gtacggcagc gtcagcgttg
      241 ccatgcccgc cgcgcggcct gccagcgcat cgttttccga atcgccgata gcgaccgtgg
      301 cttgcggcgc gacgcctagc cgcgccgcgg cggtgagcat cggcagcgga tccggcttct
      361 tttgcggcag actgtcgccg ccgagtacga cgctgaaata ctggctgagc ccgtattgct
      421 ccagcaattc gaccgcgaaa cggtgcggtt tgttggtgac gcaggctagt ttgaggccgg
      481 cgtcgcgcat ggcggagagg ccggcttcga cctccgggta cacccgcgtg tgcacgccgt
      541 tgatcttggc atactccacc tgataaatgg cgagtgcctc atcgaaacgg tcctgcgcgt
      601 gatccgtttc gaagcgctga gccagcacgc tgcgaatcag attctccgag cctttgccga
      661 tgtagccgat cacttcctcg cgcgaggttt ccgtcgcgtc gagttgcgca agcatgccgt
      721 tcaggccggc ggtgaaatcg tcggcggtat cgatcatcgt gccatccagg tcgatgatcg
      781 cggcttgcag gcgcgggccg gtgaaggtcg gagcggggta tggattgttc atctttcgca
      841 gagggtctcg gtgttcgatc agtgctcgat gttggcgagc gcggcgcgca tcttgtcgat
      901 caccgccttg tgatccggcg cgccgaagat cgccgagcct gccacgaacg tgtccgcgcc
      961 agccgctgcg atttcggcga tattgtcgac cttcacgccg ccgtccactt cgagatgaat
     1021 ctcgcggccg gttttttcct tgtacgcgtt gatccgcttg cgcgcttcgc gcagcttgac
     1081 gagcgcctca ggaatgaacg actgaccgcc aaagccagga ttcaccgaca tgatcagcac
     1141 cagatcgacc ttgtccatca catagtccag atagctcagc gaggtggccg ggttgaacac
     1201 gaggccggcc ttgcaaccgt ggtcgcgaat cagcgacagt gtccggtcga tatggtccga
     1261 gccttccggg tgaaaactga tcagattcgc gccggccttg gcgaagtccg gcacgatgcg
     1321 gtcgaccggg cgcaccatca gatgcacgtc gatcggcacg ttcacgtgcg ggcggatcgc
     1381 ttcgcaaacg agcgggccga ttgtcaggtt cggcacgtaa tggttgtcca tcacgtcgaa
     1441 gtggatccag tcggcgccgg cggcgacaac gttgcggact tcttcgccga ggcgggagaa
     1501 atcggcggac agaatgctgg gagcgatgcg gaattgcgtc atggcggggg cggatgcaag
     1561 cgggaaaacg ctattttacc ggtttgtccg cttctgaacc ggggctcggg tggtcgagat
     1621 cggctccgaa agcgcgcggc aaaagtgaca aaacgcctta acagccctcg gccagaggga
     1681 tttgtccggt tgcgggcgtg ccgcgcatca agcagaatgc caaaccataa gcgccgcttc
     1741 gtcaatgcgc ccgcaaacca ttggcgttcg gggcttccca cgatttttta cacccgtttg
     1801 accagaccgg aatcaggatg agccagtacg aattcagcgt ctcggcgcag gtgcagtatc
     1861 tgcccgaaga gtcggacccg gagcgccgcc agtatgcgtt tgcatacacg ctgactatcc
     1921 gtaacagcgg ccaggtgccc gcgcagctga ttgcgcgtca ctgggtgatc accgacagcg
     1981 aaaacaacgt acaggaagta aaaggccttg gcgtggtcgg gcatcaaccg ttgctcaaac
     2041 cgggcgagca cttcgaatat acgagttggg ccgtgatcgc cacgcccgtt ggcaccatgc
     2101 gcggtgacta tttctgcgtg gcggaggacg gcgagcgctt cgacgcaccc gtgcccgaat
     2161 tcatgctacg catgccgcgt actttgcatt gaaccgaggg cgcggctcgc ggtctttaaa
     2221 aagccttacg gcgcagcttc actgagattt ctgctgcttt tgctgttcat tgctggcggc
     2281 gcgcgcggcc tttttcttgc cggacgacgt ccatacgacg ataaagacga gcaggacgag
     2341 cgcgaagagc gattccagcg cgaagatgag cattgggtat tcgtcgaaca gatcagacat
     2401 ggcggtttcc atcaagaacg aacattgtat gcggtttgtc cgcacggccg gagcatggct
     2461 cggggcgctt tcggttgcag tgctgctggc ttcctgcggt ggtggcggcg cggtccggcc
     2521 ttcggtttcg ccgcccacgg gtgccgcgat catacccggg cagatcgccg cgacgcggct
     2581 cacagcggta gcctggcagc aggtgccagg gtggcaggac gattcgctga tcggcgcaac
     2641 ggctgcgttg cggcaaaact gcctgaggct tgcgcgtcaa ccgaactggg ctcgcgcatg
     2701 cgcggcggct tcgcagatcg acgatctgga cgtgaccggc gcgcgggcgt tttttgaggc
     2761 gtacttcacg ccttttcagt tagccaacaa cgacggcacg ctcgacggtc tcgtcaccgg
     2821 ctactacgag ccattgctgc gcggttcgcg cacacggcat ggcgtgtacc agacggcgtt
     2881 gtaccgctgg ccatccggtt atcgcgcggg tgctccgctg ccgccgcgtg cgcaactcga
     2941 gcgcgagggt gtgctcaacg gcaatgaact tgtgtgggtc gacgatccga tcgaagcctt
     3001 cttcctgcaa gtgcaggggt ccggacgcat cgtgatggaa gacggcagcg tgatgcggct
     3061 cggctttggc gggaccaaca accagccgta caagtcgatc ggacggtggc tgctcgatcg
     3121 cggcgaactg acaccggcgc aggcaaccat gcagggaatc aaggcatggg cgcgtgcgaa
     3181 ccc
//
LOCUS       DATASH010000667         1740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1891229,
            whole genome shotgun sequence.
ACCESSION   DATASH010000667 DATASH010000000
VERSION     DATASH010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1891229"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..162
                     /gene="araH"
                     /locus_tag="VHV99_17960"
     CDS             <1..162
                     /gene="araH"
                     /locus_tag="VHV99_17960"
                     /EC_number="7.5.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921729.1"
                     /note="Part of the ABC transporter complex AraFGH involved
                     in the high affinity transport of arabinose; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose ABC transporter permease AraH"
                     /protein_id="HEX3637331.1"
                     /translation="TISGVLVGVLIMGSVQDAMSLMNVPTFYQYLIRGGILLLAVLFD
                     QFRRSKRVH"
     gene            246..>1740
                     /locus_tag="VHV99_17965"
     CDS             246..>1740
                     /locus_tag="VHV99_17965"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HEX3637332.1"
                     /translation="MADSNQTKKPLRSQAWFGLKDRDGFLHRSWMKNQGIPHDEFDGR
                     PVIGICNTWSELTPCNAHFRELAEYVKKGVHEAGGLPLEFPVMSLGETNLRPTAMLFR
                     NLASMDVEESIRGNPMDGVILLVGCDKTTPALLMGAASCNLPALAVSGGPMLNGRFRG
                     KNIGSGTGVWQMSEEVRAGTMTQEEFTEAESCMNRSRGHCMTMGTASTMASMVESLGM
                     GLPHNAAIPAVDARRQVLAHLAGRRIVDMVREDLTMDKILTRHAFENAIRTNAAIGGS
                     TNAVVHLIALAARIGVPLSLEDWELGSEVPCLVNLQPSGEYLMEDFYYAGGLPAVLKQ
                     LGEQGLLHRDALTVNGKTIWDNVRNAPNYDEKVITTFAEPFKPKAGIAVLKGNLAPNG
                     AVIKPSAATEALLQHRGRAVVFENIEELHAKIDDESLDIDEHCIMVLKGAGPKGYPGF
                     AEVGNMPLPKKVLQKGITDMVRISDGRMSGTAYGAVVLHVSPEAAAGG"
BASE COUNT          326 a          504 c          586 g          324 t
ORIGIN      
        1 acgatttccg gtgtgctggt cggcgtgctg atcatgggtt ccgtgcagga cgcgatgagc
       61 ctgatgaacg tgccgacctt ctaccaatac ctgattcgcg gcgggattct gctgctcgcg
      121 gtgctgttcg atcagttccg ccgcagcaag cgcgtgcact gacgcaggat gcactgacgc
      181 gcggcttcgg ccacgcgccc ccggttgggc gccccgctct acatgcttgc caacaaggag
      241 atttcatggc tgattcgaac cagactaaaa agccgctgcg cagccaggcg tggttcggcc
      301 tcaaggaccg cgacggcttt ctgcatcgct cgtggatgaa aaaccagggc atcccgcacg
      361 acgaattcga cggacgtccg gtgatcggca tctgcaatac gtggtcggaa ctgacgcctt
      421 gcaacgcgca ttttcgcgag ctcgccgagt acgtgaaaaa aggcgtgcac gaagcgggcg
      481 gtttgccgct cgagtttccg gtgatgtcgc tcggcgaaac caatttgcga cctaccgcga
      541 tgctgtttcg caacctcgct tcaatggatg tcgaggagtc gattcgcggc aatccgatgg
      601 acggcgtgat tctgctggtc ggctgcgaca agaccacgcc tgccttgctg atgggcgcgg
      661 cgtcgtgcaa tctgcccgcg ctggcggtgt ccggcggccc gatgctcaat ggccgttttc
      721 gcggcaagaa catcggctcc ggcacgggtg tctggcagat gtccgaggaa gtgcgcgccg
      781 gcacgatgac gcaggaagaa ttcaccgaag ccgagtcgtg catgaaccgc tcgcgcggtc
      841 actgcatgac gatgggcacg gcgtcgacga tggcgtcgat ggtcgaatcg ctcggcatgg
      901 gcttgccgca caacgcggcg attcccgccg tcgatgcgcg tcgccaggtg cttgcgcatc
      961 tcgcgggccg gcgcatcgtc gacatggtcc gcgaggatct gacgatggac aagatcctta
     1021 cgcggcacgc gtttgaaaac gcgattcgca cgaatgcggc gatcggcggc tcgaccaatg
     1081 cggtggtgca tctgatcgcg ctggccgcac gtatcggcgt gccgttgtcg ctggaggatt
     1141 gggagctcgg gtcggaggtg ccgtgtctcg tgaatctgca accgtcaggt gaatatctga
     1201 tggaagactt ttattacgcg ggtggtttgc cggcggtgtt gaagcagctc ggcgagcagg
     1261 ggctgctgca tcgcgatgcg ctgaccgtga atggcaagac aatctgggac aacgtgcgca
     1321 acgcgccgaa ttacgacgag aaggtcatca cgacattcgc cgaaccgttc aagccgaagg
     1381 cgggcattgc ggtgctcaag ggcaacctcg cgccgaacgg cgcggtgatc aaaccttccg
     1441 cggccaccga ggccttgctc cagcatcgcg gccgcgcggt ggtgttcgag aacatcgagg
     1501 aactgcacgc aaagatcgac gacgagtcgc tcgacatcga cgagcattgc atcatggtgc
     1561 tcaagggcgc gggaccgaag ggctatccgg gcttcgcgga agtcggcaat atgccgctgc
     1621 cgaagaaggt gctgcaaaaa ggcatcacgg atatggtgcg aatctcggac gggcgcatga
     1681 gcggcaccgc gtatggcgcg gtggtgctgc atgtgtcgcc ggaggccgca gccggcggcc
//
LOCUS       DATASH010000668         3014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3669748,
            whole genome shotgun sequence.
ACCESSION   DATASH010000668 DATASH010000000
VERSION     DATASH010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3669748"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..1321
                     /locus_tag="VHV99_17970"
     CDS             29..1321
                     /locus_tag="VHV99_17970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HEX3637333.1"
                     /translation="MAATTTPIRRQPERRAEHPDDNARPAAPAVMPPGLRHADDTRPG
                     YTRKREKDGFVYFDLAGERIDDEDEIKRINALAIPPAYEDVWICPDARGHIQATGRDA
                     RGRKQYRYHPRWRETRDADKYERMAQFGRALPKIRARVARDLKLAGMPCAKVIAAIVQ
                     LLDTTLIRIGSVEYARDNQSYGLTTLRKKHVKIEAGQLRFRFRGKSGIEHDVTVDNPR
                     IKRIVRRCAELPGHELFQYLDEDGTRRTIGSADINEYLRRASDADFTAKDYRTWAGSV
                     YALAALRRLMCGSAAEARRHIVATVKDVAVLLRNTPAVCRRCYIHPVIISAFEADELQ
                     SLPPGQARRGLRVDEVAFAALLAHAEKRAAQQARKAGAKGQTMREAAVTDSVNGSLTS
                     LLKKSRVVRREAVAKTDAKAGAQTARRPDSAVRPTKMQ"
     gene            complement(1391..1645)
                     /locus_tag="VHV99_17975"
     CDS             complement(1391..1645)
                     /locus_tag="VHV99_17975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637334.1"
                     /translation="MKQRFSDNVGKQALMVAAIVLLTAASGCKRSDSDSTGASSAMSS
                     SGVMNGNAATGSTADTSSGAAAAAAGASAPGAAAASGASQ"
     gene            complement(1730..2023)
                     /locus_tag="VHV99_17980"
     CDS             complement(1730..2023)
                     /locus_tag="VHV99_17980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637335.1"
                     /translation="MKYSKALLVSALAAMSGVAFAQAGGGNGNGGNGGTGGSGNAHGA
                     ATAGPTAGGEPASGAMSSGTSKMHHTSKSKAKKPMSDSTNMPGADASSDTKGQ"
     gene            complement(2077..2283)
                     /locus_tag="VHV99_17985"
     CDS             complement(2077..2283)
                     /locus_tag="VHV99_17985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637336.1"
                     /translation="MERAARSISRVLGGTQERRNAGTQERRNAENAENAENAENAETP
                     KRRAPSHMVTHSAILIDSGRHNPC"
     gene            2531..>3014
                     /locus_tag="VHV99_17990"
     CDS             2531..>3014
                     /locus_tag="VHV99_17990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX3637337.1"
                     /translation="MLNQTQTQGGMRQSQGARIVGTGSASADGPGPDVMAAATLDGDK
                     VVSSDGEDIGKVKDIMLDVGSGRIAYAVLSSGGFLGIGNKLLAIPWSALTLDVEHKCF
                     RIDATSEEIRHAPGFDKESWPSMADRMWASSIHQYYGREPYWKVDAGAAENLENTYAG
                     P"
BASE COUNT          564 a          952 c          919 g          579 t
ORIGIN      
        1 cagctgcttg aaacttctac ctgcgacgat ggctgccacg accacaccga ttcgacgcca
       61 acccgaacgc cgtgccgaac acccggacga caacgcgcga ccagccgcgc ctgcggtcat
      121 gccgccaggc ttgcggcacg ccgacgacac caggcccggc tatacgcgca agcgtgaaaa
      181 agacggcttc gtatatttcg atctggcggg cgagcgcatc gacgacgaag acgagatcaa
      241 acgcatcaat gcgctggcca ttccgccggc ttacgaagac gtctggatct gtcccgacgc
      301 gcgtggccat attcaggcga cgggccgcga cgcacgcggg cgcaagcaat accgctatca
      361 ccctcgctgg cgcgaaacac gcgacgccga caaatacgaa cgcatggcgc agttcggccg
      421 cgccttaccg aagatccgcg cacgcgtggc acgcgatctg aagctggcgg gcatgccatg
      481 cgccaaggtc atcgccgcga tcgtgcaatt gctcgacacc acgctgatcc gcatcggcag
      541 tgtggaatac gcgcgtgaca accagtcgta cggtttgacg acgctgcgca agaagcatgt
      601 gaagatcgaa gccggccagt tgcgtttcag gtttcgcggc aagagcggca tcgagcacga
      661 cgtgaccgtg gacaatccac gcatcaaacg catcgtgcgc cgctgcgccg agctgccagg
      721 tcacgagttg tttcagtatc tcgacgaaga cggcacgcgc cgcacgatcg gttctgccga
      781 catcaacgaa tatctgcgcc gcgcgagcga tgccgacttc accgcgaagg actatcggac
      841 gtgggccggc agcgtctatg cattggccgc gctgcggcgc ttgatgtgcg gcagcgcggc
      901 ggaagcgcgc cgccatatcg ttgcgactgt taaggacgtg gcggtgctat tgcgcaatac
      961 ccctgcagtt tgccgccgtt gctacattca tcccgtgata atcagtgcgt tcgaggcgga
     1021 cgagttacag agcttgccgc ccggccaggc acgccgtgga ctgagagtgg acgaagtggc
     1081 ctttgccgcc ttgctcgcgc atgcggaaaa acgcgcggcg cagcaggcgc gcaaggccgg
     1141 tgccaagggc caaacgatgc gcgaggcggc ggtgacggac agcgtcaacg gctccctgac
     1201 cagtctcctg aaaaagtcgc gcgtggtgcg tagagaagcg gtggcgaaga ccgatgccaa
     1261 ggcgggtgcg caaaccgcga ggcggccgga ctctgctgtg cggccgacca aaatgcagtg
     1321 aaattccggg gcggcgattc tttgccgcac gccagaagac tgtgctgttc ccttcggcct
     1381 cgtatctaac tcattgactc gcgcctgatg ccgcggccgc gcccggcgcg cttgccccgg
     1441 cggcggccgc cgccgcaccg ctactcgtgt ccgctgtgct gcctgtcgcc gcgttaccgt
     1501 tcatcacgcc actgctgctc atcgcggaac tcgcccctgt gctgtcgcta tcggagcgct
     1561 tgcagccgga cgcggccgtc agcaatacga tagccgccac catcagcgcc tgtttgccca
     1621 cgttgtcgct gaatcgttgt ttcatcactg cctcctgatc gatagggcga aaccgctccc
     1681 gtgctaaacg agcaccgcct cgcctgccct cactcacttt tagcgctatt cattgaccct
     1741 tggtatcgct gctcgcgtcg gcgcctggca tgttggttga atcgctcatc ggcttctttg
     1801 ctttcgattt cgaggtgtga tgcattttcg acgtgccgga actcatcgca cccgacgccg
     1861 gttcgccacc cgccgtcggc cctgccgttg ctgcgccgtg cgcgttaccc gaaccgccgg
     1921 tgccaccgtt gccgccgtta ccgttaccgc cgcccgcttg cgcgaaagcg acgcccgaca
     1981 ttgcggcgag tgccgacacg agtaatgcct ttgaatattt catcgaaatc tccctgagtg
     2041 aatggatgcg tgaatagcga gaacgcagca agactttcag caagggttat gcctcccgga
     2101 gtcgatcagg atggctgaat gcgtgaccat gtgagacggc gcccggcgtt tcggcgtttc
     2161 ggcgttttcg gcgttttcgg cgttttcggc gttttcggca ttcctgcgtt cctgcgttcc
     2221 ggcgttcctg cgttcctgcg ttcctccaag cacgcggctg attgaccgtg cggcgcgttc
     2281 cactcgtctc ctgctcgcag gtttatcggt ccctggcttc cgctacccgt ttatcagttc
     2341 tcctcgccga gatagacgaa ataatttcaa agagatcagc atctttacgc acaatttgca
     2401 cgcctcgttg gagacgttgt ttgaacggac gcccgcagtt cgccggtacc gcttatgcag
     2461 cggttctgca accgggagga acggcggttg ctggcttctc cgtacatcga aacggtaaag
     2521 gaggaacatc atgctcaacc agacacaaac ccaaggcggc atgcgccaga gccaaggcgc
     2581 ccgtatcgtc ggcacgggca gcgcgagcgc cgacggtccc ggtccggacg tgatggcggc
     2641 agccaccctc gacggcgaca aagtggtcag cagcgatggc gaggacatcg gcaaggtcaa
     2701 ggacatcatg ctcgatgtcg gctcgggccg catcgcgtac gcggtgttgt cgagcggcgg
     2761 ctttctcggg attgggaaca aattgctggc gattccctgg agcgcgttga ccttggatgt
     2821 cgagcacaag tgcttccgaa tcgatgcgac gtccgaagag attcgtcatg cgccgggctt
     2881 cgataaagaa tcctggccgt cgatggccga tcgcatgtgg gcgagttcga tacatcaata
     2941 ttatggccgc gagccctact ggaaggtcga tgccggcgcg gcggaaaatc tcgaaaatac
     3001 gtatgccggg ccgc
//
LOCUS       DATASH010000669         3642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1693524,
            whole genome shotgun sequence.
ACCESSION   DATASH010000669 DATASH010000000
VERSION     DATASH010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1693524"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VHV99_17995"
     CDS             <1..331
                     /locus_tag="VHV99_17995"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX3637338.1"
                     /translation="LLAGRVRTTDTVARLGGDEFVVLARHVTGNHAVQTVANGVHAVL
                     AEMTLVQDRVVNVSASIGACLISPDLSPGYRDIAVLMRAADRAMYRAKALGKGQTEFA
                     READFET"
     gene            complement(364..1095)
                     /locus_tag="VHV99_18000"
     CDS             complement(364..1095)
                     /locus_tag="VHV99_18000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012492271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HEX3637339.1"
                     /translation="MYNAQGKLSQSEVLTRYAPLVRRLGLQLVAKMPASVDLDDLIQA
                     GMIGLLDAASRYKEDQGAQFETYASQRIRGAMLDELRANDWLPRSMRKTSREVESAVH
                     KVEQHLGRSASEQEVAEHLNMPLIEYQAMLQDLHGSQLIYYEDFDRSSDDEPFLDRYC
                     VDKADPLSALLDGSLRSALVEAIDKLPEREKLLMSLYYEKGLNLREIGAVMEVSESRV
                     CQLHSQAVARLRAKLREQAWSGVEA"
     gene            complement(1126..2058)
                     /locus_tag="VHV99_18005"
     CDS             complement(1126..2058)
                     /locus_tag="VHV99_18005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HEX3637340.1"
                     /translation="MDKFESDQAEGLRRLLTRNGSRVVAVCGGPAGGMGGTGNTSTVV
                     NLASALAAQGKDVLVIDERQNALSASRLAKVPDNGALGAVMSGKRFLQDAVTRTPFGF
                     SVIAAPRDERISQDAAHSRVLLDGPADIVLIDTQLDRSGALSNLAVQAHDFVIVTRVA
                     PAAITEAYACIKRLHYAHAIGRFRVVVNHVKNGADAQIAFDNLAGVANRYLAVQLMPA
                     GCVAEDPRVARAHELSRCAVEAFPATAAARDYRQIAAELQYWPMPQASQFEWPVQSLR
                     AETPYGLEHSINSGAVRRTETPLMQFAMSSAASV"
     gene            complement(2051..>3642)
                     /gene="flhF"
                     /locus_tag="VHV99_18010"
     CDS             complement(2051..>3642)
                     /gene="flhF"
                     /locus_tag="VHV99_18010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HEX3637341.1"
                     /translation="DGSVEIVAVADGDLAKISPKAAAQHREMKLRESAGNHQLLTQPR
                     SNSNASPNPYASGDVFSSVFGANPEPLDDLEPEAQADDASRTMAESNPWLIEHARRVA
                     QSAAESAPQRALSASSAIAKGLGNPVTVTPQESSQAPGWTREAAQLAARRAEQKTAQA
                     QFQQEPHTSAAQTASSMQEGAFAGLPASAAAAVTEAIRTRMEQVVNDTVMNELSSMRG
                     MMEEHFAGLLWGDRQRRSPNHAALTKRLFAAGFSAQLVRLLIDNMPEIEQAEASMEWV
                     QQVLENNLPVMDSEESMMNRGGVFALMGPTGVGKTTTTAKLAARCVMRFGASKVALLT
                     TDSYRIGAHEQLRIFGKILGVSVHAVKDAGDLQLALSELRNKHIVLIDTIGMSQRDRT
                     VSDHIAMLCGAGRPVQRLLLLNATSHGDTLNEVVQAYQSTPDQPPLAGCILTKLDEAT
                     NLGGVLDTVIRYKLPVHYVSTGQKVPENLYVATRRFLIKSAFCIPRDGSPFVPQDDDV
                     PPLLSALSARASTELHEVHFG"
BASE COUNT          633 a         1154 c         1149 g          706 t
ORIGIN      
        1 gcttctggca ggaagggtaa gaaccaccga cacagtcgcg cggctcggcg gcgatgaatt
       61 cgtcgtgctg gcgcggcacg tgaccggcaa tcacgccgtg cagacggtgg caaacggcgt
      121 gcacgcggtg ctggcggaaa tgacgttggt gcaggaccgg gtggtgaatg tctcggcgag
      181 tattggtgct tgcctgattt cgccggactt gagccccggc tacagggata ttgccgtgct
      241 gatgcgcgcc gccgatcgcg cgatgtatcg cgccaaggcg ttgggcaaag gccagacgga
      301 gttcgcgcgg gaagcggatt tcgaaacctg atcgctcttc atcggccttt caatcgcggg
      361 agctcaagcc tccacgcccg accacgcctg ttcccgcaac ttcgcccgca gccgcgcgac
      421 ggcctggcta tgcaactggc atacacgcga ttcgctgact tccatcacgg cgccaatttc
      481 gcgcaaattc agccccttct cgtaatacag gctcatcaac agcttttcgc gttcaggcaa
      541 tttatcgatt gcctcaacca gcgccgagcg caagctgccg tcgagcaacg cggagagcgg
      601 gtcggcttta tcgacgcaat aacggtcgag gaacggctcg tcatcggaag aacggtcgaa
      661 gtcttcgtaa tagatcaact ggctgccatg cagatcctgc agcatggcct ggtattcgat
      721 cagcggcatg ttcagatgct cggccacttc ctgttcgctc gccgaccggc cgagatgttg
      781 ctctacctta tgcaccgccg attccacttc gcgcgaagtc ttgcgcatgc tgcgcggcag
      841 ccagtcgttg gcacgcaact cgtcgagcat cgcgccgcga atccgctggc tggcgtaggt
      901 ctcgaactga gcgccctgat cttctttata gcggctggcg gcatcgagca aaccgatcat
      961 gccggcctgg atcaggtcgt ccagatcgac gctagccggc attttcgcca cgagctgcag
     1021 gccaagccgg cgcaccagcg gtgcgtagcg ggtcaggacc tcggactgtg acagttttcc
     1081 ctgggcgtta tacatcgcat tcaccttgtt ccttctcttg cggcatcaca cggacgcggc
     1141 gctgctcatc gcaaactgca tcaatggcgt ttccgtgcgg cgtaccgcgc cggagttgat
     1201 cgagtgctcc aacccgtacg gcgtttccgc tcgcaaggac tgcacgggcc attcaaactg
     1261 cgacgcttgt ggcatcggcc agtactgcaa ttcagcggca atctgccgat aatcccgggc
     1321 ggcggccgtc gccggaaaag cttccaccgc gcaacgcgat agttcatgcg cccgcgccac
     1381 gcgcggatct tcagccacgc aacccgccgg catgagttgc accgcgagat accggttggc
     1441 gacgccggcg aggttatcga aggcgatctg cgcgtcggcg ccgttcttca cgtgattcac
     1501 gacaacccgg aaccggccga tggcgtgcgc gtagtgcaga cgcttgatgc aggcgtacgc
     1561 ctcggtgatc gcggcaggcg ccacgcgggt cacgatcacg aagtcgtgcg cttgcacggc
     1621 caggttcgac agcgcgccgc tgcgatcgag ctgggtatcg atcaggacga tatcggcggg
     1681 tccgtcaagc agcacccggc tatgcgcggc gtcctggctg atgcgttcat cgcgaggcgc
     1741 ggcgatcacc gaaaagccga acggcgtgcg cgtgacggca tcctgcaaaa aacgcttgcc
     1801 ggacatgacc gcgcccagtg caccgttgtc cggcaccttt gccagccggc tcgccgataa
     1861 cgcgttctgg cgttcatcga tcacgagcac gtccttgccc tgcgccgcca gcgcggacgc
     1921 gaggttgacg accgtcgagg tgttgcccgt tccacccatt ccgccggccg ggccgccgca
     1981 cacggcgacg acccgcgagc cgttgcgcgt cagcaggcgg cgcaagcctt cagcctgatc
     2041 ggactcgaac ttatccaaag tgcacctcat gcagttcggt ggaagcgcgc gcggagagcg
     2101 ccgacaggag cggcggcacg tcgtcgtctt gcggcacgaa cggcgagccg tcgcgcggaa
     2161 tgcagaaggc gcttttaatc aggaagcgcc tggtcgcgac gtacaggttt tccggcactt
     2221 tctgacccgt cgatacgtaa tgcacaggca gcttgtagcg gatcacggtg tcgagtacgc
     2281 cacccaggtt cgtggcttcg tcgagcttgg tcaggatgca gcccgcgagc ggcggctggt
     2341 ccggcgtgct ctggtaggcc tgcactactt cgttcaacgt atcgccgtgg ctggttgcgt
     2401 tcagcagcag caggcgttgc accggacggc cggcgccgca caacatggcg atgtgatcgg
     2461 agactgtgcg gtcacgctgg ctcatgccga tcgtgtcgat cagcacgatg tgcttgttac
     2521 gcagttcgga gagcgcgagt tgaaggtcgc ccgcgtcttt aaccgcatgc accgataccc
     2581 cgaggatctt gccgaaaata cgcagttgtt cgtgtgcgcc gatccgatag ctgtcggtgg
     2641 tgagcagcgc aaccttgctg gcgccgaagc gcatcacgca acgcgcggcg agcttggcgg
     2701 tggtggtggt cttgcccacg cccgtcgggc ccatcaacgc gaaaacgccg ccacggttca
     2761 tcatcgattc ttcgctgtcc atgacgggca ggttgttttc gagcacttgc tgaacccatt
     2821 ccatcgacgc ttcggcttgt tcgatctccg gcatgttgtc gatcagcaaa cggaccagtt
     2881 gcgcggagaa accggcggcg aacagacgct tcgtcagcgc agcgtgattc gggctgcgac
     2941 gctggcgatc gccccacagc aagccggcga agtgctcttc catcatgccg cgcatcgacg
     3001 aaagttcatt catcacggtg tcgttcacga cttgctccat gcgggtgcgg atggcttcag
     3061 tcacggcagc cgccgccgat gccggcaggc ccgcgaacgc gccttcctgc atcgaactgg
     3121 cggtttgggc cgcggacgta tggggttcct gctggaattg cgcctgcgcg gttttttgtt
     3181 ccgcacggcg ggcggcgagt tgcgccgctt cccgggtcca accgggggct tgggaggatt
     3241 cctgcggcgt gacagtcacg ggattgccca atcccttcgc aatcgccgac gatgcgctca
     3301 gcgcgcgttg cggcgcactt tcagcggccg actgcgccac ccggcgggca tgctcaatca
     3361 gccagggatt cgattcggcc atcgtgcgcg acgcgtcgtc tgcttgcgct tcgggctcga
     3421 gatcatccag cggttcggga ttggcgccga acaccgacga aaagacatcg ccgctcgcgt
     3481 aagggtttgg acttgcgttc gaattcgacc gcggctgcgt aagcaattga tgatttccgg
     3541 ccgattcccg caacttcatc tcgcggtgct gagcggcggc tttcggggag atcttcgcaa
     3601 ggtcgccgtc cgcgaccgca acgatctcga cgctgccgtc ct
//
LOCUS       DATASH010000670         1608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2877828,
            whole genome shotgun sequence.
ACCESSION   DATASH010000670 DATASH010000000
VERSION     DATASH010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2877828"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VHV99_18015"
     CDS             complement(<1..251)
                     /locus_tag="VHV99_18015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008353617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX3637342.1"
                     /translation="MATVAQVLNSKPDHTIFTIADTASVYEAIAMMAEKHIGALIVTH
                     GEAIVGIMTERDYARKVVLMDRVSRQTPVREIMTSQVRFV"
     gene            641..1603
                     /locus_tag="VHV99_18020"
     CDS             641..1603
                     /locus_tag="VHV99_18020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008343353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HEX3637343.1"
                     /translation="MSSSQHDIEQRVRLAGNNMFELLLFRLGEAPDSEQRELYGINVF
                     KVREILAMPPITVVAGANPDILGVADIRGQIIPVIDLAQVTGCQPKNGLKILLVTEFA
                     RTTQGFAVEEVDDIVRLEWNQVLSAESHSSGRTVTSFARLDGNVDGSRLAQVLDVEQI
                     LRDVLPSTEVEINESSTGRIILKPGTKVLAADDSGVARSLIEQSLSAMGVQFIMTKSG
                     QEAWNVLEDLQSKAEKHGRCAADEIALVLTDLEMPEMDGFTLTRRIKANAGMQAIPVV
                     IHSSLSGSANEEHVRKVGANAYVSKFAAGELAAVMRDALKMTGT"
BASE COUNT          354 a          473 c          483 g          298 t
ORIGIN      
        1 acgaagcgca cttgcgaagt catgatttcg cgcaccgggg tctgccgtga aacacggtcc
       61 atcagcacga ctttgcgggc atagtcacgc tcggtcatga tgccgacgat cgcttcgcca
      121 tgggtcacga tcaacgcgcc aatgtgcttc tccgccatca tcgcgatagc ctcgtagacc
      181 gatgccgtgt cggcaatcgt gaagatggtg tgatcgggtt tcgaattaag aacttgtgcg
      241 actgttgcca tcgaacgcct ccgttgaacc gcgcgacgcc tgccggctgg cgggcgtttc
      301 acatgggtgg acagctggca tccggaaacg cacgacgcgt ttcacaacgg gcttgccgaa
      361 tccggccgcg ttggatcaat cgtcttacgt cccgcgcctt acatccatat tacccagccc
      421 attaaaaaag tataggccag agacctgctc cgtgaacgct ggaaaacact gctttttatg
      481 cgttcaaggg tctatcccga tgcagccgca cgcgcgagcg gactggcatg caacagtccc
      541 gtcacaaagc atcgaaaagc gcttcagttt ccccgcaccg cgccgctaat caggaaccgg
      601 ctatttagaa gcccggcata aatcaatcag gagaaccacc ttgagcagtt cacaacacga
      661 catcgaacag cgggttcggc tcgcgggcaa caacatgttc gaactgctcc tgtttcgcct
      721 gggcgaagcg ccggactccg aacaacgcga gctatatggc atcaacgtgt tcaaggtgcg
      781 cgaaatcctc gcgatgccgc cgatcaccgt ggtcgccggc gcaaacccgg acattcttgg
      841 cgtagccgat atccgcggtc agatcatccc cgtgatcgat ctggcgcagg tcacgggctg
      901 ccagccgaag aacggtctca agatcctgct ggtcaccgaa ttcgcgcgca cgactcaagg
      961 ttttgctgtc gaggaagtcg atgacatcgt gcggctggag tggaatcagg ttttgtctgc
     1021 ggaatcgcat tcgagcgggc ggacggtgac cagtttcgcg cgcctcgacg gcaacgtgga
     1081 cggctcccgg ctcgcgcagg tactcgatgt cgaacagatc ctgcgcgacg tgctgccgtc
     1141 gacggaagtg gagatcaatg agtcaagcac tggccggatc atcctgaaac caggtaccaa
     1201 ggtgctcgcc gccgacgact cgggcgtcgc gcgcagcctg atcgaacaaa gcctgagcgc
     1261 gatgggcgtg cagttcatca tgaccaagag cggccaggaa gcgtggaatg tgctggaaga
     1321 cttgcagagc aaggcggaaa aacacggccg atgtgcggcg gacgaaatcg cgctcgtcct
     1381 gaccgatctc gaaatgccgg aaatggacgg attcacgctg acgcgccgta tcaaggcgaa
     1441 cgccggcatg caggccattc cggtcgtgat ccactcgtca ttgtccgggt cggcgaacga
     1501 ggagcacgtg cgcaaggtgg gcgcgaacgc ctatgtgtcc aagtttgccg cgggagaact
     1561 ggcggcggtg atgcgcgatg ccctcaagat gacaggcacc tgaaacag
//
LOCUS       DATASH010000671         5518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2087987,
            whole genome shotgun sequence.
ACCESSION   DATASH010000671 DATASH010000000
VERSION     DATASH010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5518
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2087987"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VHV99_18025"
     CDS             complement(<1..345)
                     /locus_tag="VHV99_18025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492120.1"
                     /note="c-di-GMP phosphodiesterase; probably degrades
                     signalling molecule c-di-GMP; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HEX3637344.1"
                     /translation="MNDDQDDQVLFAQFGTSSPCWRLSNDSNALELTPVTGDVPANVA
                     IPLNPQQASQIRCLTGVTSHLILDVRLFGELLRLHIVGKKLASNTWGGTASAYDDTES
                     VARDLVHGLSFAE"
     gene            complement(742..2115)
                     /locus_tag="VHV99_18030"
     CDS             complement(742..2115)
                     /locus_tag="VHV99_18030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637345.1"
                     /translation="MWSSDASNNLILSQSTGDVAATSICPIPDGGFYVSWLDSANGYS
                     LYLQRLDVSGNAMWEANGKLIYQRDIRSFIGYGLSIDSDGNAIVGIDSSIAALHAPGG
                     RVLAFKISPAGELLWGSTGITLSPSDEDVSVVLCCGTSDRGAAFIWGKLEQEDLIRIT
                     KLDSAGNQLWSTNVARDTPDAGLTPTNIVASDDGSVIYAFIHAINKMSGTYDSVLAQK
                     LAAGDGANLWGSTPVTVVDSSVATAVTLPMGEVPTLISDGAGGAVFGYPLANNTIQRI
                     YAQRVNPTGGTSYAPNGVLVSGNAIDASAASFGFDPGSGRLYIAWNANYTGMSGVRAQ
                     CVDNAGALLWDTAGIALATYEPLALMSPVAMLPVTGGVMAAWIQQPPQAASLPQPLRV
                     SMLDPSGAPVWPNPVVDLKSPATNTTDNAVGAVGMTGYGAFAWTSNDYTVRAQNINLD
                     GTLGAAT"
     gene            complement(2339..>5518)
                     /locus_tag="VHV99_18035"
     CDS             complement(2339..>5518)
                     /locus_tag="VHV99_18035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEX3637346.1"
                     /translation="LFDMMRRAAPYAELEREQYDAVLRVLAEGYTSRNGPRGAYIHRD
                     VVSGTLRGRRGGKLVAVTSGGTIPENADYAVVLEPQAINIGTVNEDFAVESLAGDVFQ
                     LGNASYRILRIESGRVRVEDAQGQPPNIPFWLGEAPGRSDELSFGVARLREQIGRLLD
                     GKQADTDTDTDSDTNTDSDSDSETNTEVMLARLQRAIDWLIDTLGLDEAAARQIVDYL
                     ARARAALSVLPSQNTLVMERFFDESGGTQLVIHAPFGSRVNRAWGLALRKRFCRTFNF
                     ELQAAATEDAIVLSLTGSHSFVLDEVWRYLHSNSAEHLLIQALLDAPLFGVRWRWNAT
                     TALGLPRYTGGRKTPPQLQRMRSEDLLTSVFPEQAACLENVVGERELPHHPLVDQTVD
                     DCLHEAMDSDSWIALLRRIEQGDVQLIARDLPAPSPLAAEILNAKPYAYLDDAPIEER
                     RTQAVLNRRWTDPSSADDLGALDADAIDSVRDEAWPQARTADEMHEALTGLACITEAE
                     ARHSEGWPAWLASLAESGRATLLRIAADDALWLPAERLTCFQALYPDAPFQPPLSAPK
                     GYADSWSADDALLDVLRARLTGFGPLPVRVIAEALKLPAASVDHALTRLEAEGYVMRG
                     RFSPAATEEEWCERHLLARIHRYTVKRLRREIEPVERHDFMRFLFEWQHLTPDTRSEG
                     RDALAAVLDQMEGFQAAAGAWEEDILPARVKAYANVSLDELCCAGKIVWTRLTERARG
                     AAGPVRSTPIVLLPRAQVRVWRALLDLSKQPELSARAQSVHDTLAQHGAMFFDELLTE
                     VGGLRMELESALGELVAAGLVNSDSFAGLRALLKPVAKRSAYSSSRRVRSSALIGGMD
                     DAGRWALIKRPAKAGPERANGNGDDNGSENGGRPALPVNGRRAPLAPEILEHIAMTLL
                     RRYGVVFWRLLEREAEWLPPWRDLLRVFQRLEARGQVRGGRFVNGLAGEQFALPEAIP
                     LLREVRRHANDDVWGCVAATDPLNLAGTLLTGDKVPAVAGNRLLFRDGILVATLVAGQ
                     FNFLPALGADTDTDAEAKTQARMRLARRY"
BASE COUNT          983 a         1709 c         1752 g         1074 t
ORIGIN      
        1 ttcggcgaac gaaagaccgt ggaccagatc gcgcgcgacg gattcggtgt cgtcataagc
       61 cgaggcggtg ccgccccacg tattgctggc aagtttttta ccgacgatgt gcaggcgcag
      121 gagttcgccg aacagccgca cgtcgaggat gagatgcgag gtgacgccgg tcaggcaacg
      181 gatttgcgaa gcctgttggg gattcagagg aattgcgaca ttggcgggca cgtcgccggt
      241 aacaggcgtg agttccagtg cgttgctgtc gttcgacaga cgccagcaag ggctgcttgt
      301 gccgaactgc gcaaaaagca cctgatcgtc ttggtcgtcg ttcatgagat ccccggggcg
      361 tgtgtgccga tgagtccgtc gcgaaggcgg gatggcggca aacagtcttc atcatactta
      421 aaagaccggc cccattgtag ctaagcggct ggcgttcggg cctattaaca ttaacggaaa
      481 tgtatgcctt cctctttaag gcttttttgt tggggaatcc gtgcaagcgc gttgcatgcc
      541 agtttgtttc gccctgccga ggcctggctg gcggcttcgt tcaatacacg cacaggcgtg
      601 ctttttttca cttgctgggg gagaagcacg ctttgtcgct gtgctgaggc cgagggacgg
      661 cacagtggtt tgtagagttg gaagcgcttt cagtctgcaa tgtcggcgct tccttcttgc
      721 ggcgtgctgt taagtccgat gttatgtcgc cgcgccaagc gtgccgtcga ggttaatgtt
      781 ctgcgcgcgt accgtatagt cgttgctggt ccatgcaaac gcgccatagc ctgtcatgcc
      841 gactgctcca acagcgttgt ccgtagtatt cgtggcggga gacttgaggt ccacaaccgg
      901 gttcggccag accggcgcgc cgctcggatc cagcatggac acacgaagcg gttgcggtaa
      961 agaggccgct tgcggcggtt gctgtatcca cgcggccatt acgccgcccg tcacgggcaa
     1021 catggctacc ggcgacatga gggcaagggg ttcatacgtt gcgagtgcaa tgccggccgt
     1081 gtcccacagc agcgcgccgg cattgtcgac gcactgtgcg cgcacgccgg acatgcctgt
     1141 gtaattggcg ttccacgcaa tataaagcct gccgctgcct ggatcgaagc cgaagctcgc
     1201 agccgaagcg tcgatcgcat tccccgagac cagcacgccg ttcggtgcgt acgatgtccc
     1261 gccagttgga ttcacgcgtt gtgcgtagat tcgttgaatc gtattgttgg cgagcggata
     1321 accaaacacg gccccgccgg ctccgtcgga gatcagcgtt ggcacttcgc ccatcggtaa
     1381 tgtcacggcg gtggccacgc tgctgtcgac gaccgtgact ggcgtgctgc cccaaagatt
     1441 ggcgccgtcg ccggccgcaa gcttctgtgc gagcacgctg tcgtaagtac cgctcatctt
     1501 gttgatggca tgaataaagg cgtagatgac cgaaccgtca tcggacgcta cgatgttcgt
     1561 gggtgtgagt cccgcatctg gcgtatcgcg tgccacattc gtggaccaca actgattgcc
     1621 tgccgaatcg agcttcgtta tgcgaatcag gtcttcctgt tcgagtttcc cccagataaa
     1681 cgccgcccct cgatcgcttg tcccgcaaca gagcacgaca gaaacgtcct cgtctgacgg
     1741 cgacagcgtg atgccggttg agccccacaa caattcacct gcgggcgaaa tcttgaatgc
     1801 aagcacccga ccgcccggcg catgaagcgc tgcaatgctc gagtcgatgc cgacaatcgc
     1861 gttgccgtcc gaatcgatgc tcaagccata gccgatgaag ctgcggatgt cgcgttgata
     1921 gatcagcttg ccattggcct cccacattgc gttgccactt acgtcgagac gctggagata
     1981 taacgagtac ccgttggcgc tgtcgagcca actgacatag aagccgccgt cgggaatagg
     2041 acagatcgat gtcgcggcga catctccggt tgattgcgac agaatgagat tgttcgatgc
     2101 atcactggac cacattgtct gctcccttcg aatgcgccga cgcgtcgcgt ccccgccggt
     2161 cgcgtggact acgataaaga tgggttatcg gtgtggcggg cagctaatcg aaacggataa
     2221 cgacgctatt tctgacgttg cgttgatttt tctacccgga gaggagtctc tcagaaatgg
     2281 aaattatttg gcatcctgaa tatgggctgc gcggcgtggc aaatcggcgc acgactcgtc
     2341 aatagcgccg cgcgagtcgc attctggcct gtgtctttgc ctctgcgtcg gtgtcggtgt
     2401 cggcgccgag cgcgggcaga aaattgaatt gtccggccac cagtgtggca accaggatac
     2461 cgtcgcgaaa cagcaggcgg ttgccggcca ccgccggtac cttgtcaccg gtcagcaagg
     2521 tgccggcgag attgagcgga tcggttgccg ctacgcaccc ccagacatcg tcgttcgcat
     2581 gccggcgcac ttcgcgcagc aaggggatcg cctcgggcaa cgcaaattgt tcccctgcga
     2641 gcccattgac gaagcgcccg ccacgaacct ggccccgcgc ctcgagtcgc tgaaagaccc
     2701 gcagcagatc gcgccatggc ggcagccatt cggcttcgcg ttcgagtaga cgccagaaca
     2761 cgacaccgta gcggcgtagc agcgtcatcg cgatgtgttc gaggatctcc ggtgcgagcg
     2821 gcgcgcgccg tccattgacg ggtaacgcgg gccggccgcc gttttcgctg ccattgtcgt
     2881 cgccattgcc attggcgcgt tcgggcccag ctttcgccgg ccgtttgatg agcgcccagc
     2941 gtcctgcgtc gtccatgcct ccgatcaatg cactcgacct gacgcgtcgg ctactcgaat
     3001 aagcactgcg tttggcgacc ggcttcaaca acgcccgcaa gccggcgaaa ctgtcggaat
     3061 tcacgaggcc cgccgcaacc agttcgccca gcgcactctc cagttccatc cgcaagccgc
     3121 cgacctcagt cagcaattcg tcgaagaaca tcgcgccatg ttgcgctaac gtgtcatgga
     3181 cgctttgtgc gcgagccgat agctccggtt gtttcgacaa gtcgagcaac gcgcgccaca
     3241 cgcgcacctg agcacgcggc aaaagcacga tcggcgtgct gcgcaccggt ccggcggcac
     3301 cgcgtgcccg ctcggtcaga cgggtccaga cgatcttgcc ggcgcagcat agttcgtcca
     3361 gcgaaacgtt tgcatacgcc ttcacgcgcg ctggcagaat atcttcttcc catgcgcctg
     3421 ccgcagcctg aaaaccctcc atttgatcga gtacggcggc caacgcgtcg cgtccctcgc
     3481 tgcgggtgtc cggtgtcaaa tgctgccact cgaacagaaa acgcatgaag tcgtgccgtt
     3541 ccaccggctc gatctcgcgc cgcaggcgtt tcactgtgta acggtgaatc cgcgccagca
     3601 aatgacgctc acaccattct tcctcggttg cggccggcga gaagcggccg cgcatcacat
     3661 agccttccgc ttcgagacgc gtcagcgcgt ggtcgaccga tgccgccggc aacttcagcg
     3721 cctctgcaat cacgcgcacc ggcagcgggc cgaagccggt caaccgggcg cgcagcacgt
     3781 cgagcagtgc gtcgtcggcg ctccagctgt cggcatagcc tttcggcgcg ctcagcggcg
     3841 gttggaacgg ggcgtcgggg tagagcgcct ggaaacaggt caggcgctcg gcgggcagcc
     3901 acagcgcgtc gtcggcggcg atccgcagaa gcgttgcgcg gccggattcg gcgagcgacg
     3961 caagccatgc cggccagcct tcgctatggc gcgcttcggc ctcggtgatg caggcaaggc
     4021 ccgtgagcgc ttcgtgcatc tcgtcggcgg tgcgcgcctg cggccatgct tcgtcgcgca
     4081 cgctgtcgat cgcgtcggca tcgagcgcgc ccaggtcgtc ggcgctggac ggatcggtcc
     4141 agcggcgatt cagcacggct tgcgtgcggc gctcttcgat tggtgcgtcg tcgagatagg
     4201 cgtagggctt ggcgttgagg atttcggcgg caagtggcga gggcgccggc aggtcgcggg
     4261 cgatcagttg cacatcgccc tgttcgatac gacgcagcaa cgcaatccag ctatcgctgt
     4321 ccatcgcttc gtgcaggcag tcgtcgacgg tttggtccac taatggatga tgcggcagtt
     4381 cgcgctcgcc gacgacgttc tccagacacg cggcctgctc gggaaacacg ctcgtcagca
     4441 ggtcttcgct gcgcatgcgc tgcaattgcg gcggagtctt gcggccgccg gtataacgcg
     4501 gcaggccgag cgcggtggtc gcgttccagc gccagcgcac gccgaacagc ggcgcgtcga
     4561 gcagcgcctg gatcagcaga tgttcggcgc tgttcgaatg caggtaacgc catacttcgt
     4621 cgagcacgaa cgaatggctg ccggtcagcg acagcacgat cgcgtcttcc gtcgcggcgg
     4681 cttgcagttc gaagttgaag gtccggcaaa aacgcttgcg caacgcgagg ccccatgcgc
     4741 ggttcacgcg gctgccgaac ggcgcatgga tcacgagctg cgtgccgccg gattcgtcga
     4801 aaaagcgctc catcaccagc gtgttttgcg acggcagtac gctcagtgcg gcgcgtgccc
     4861 gggcgagata gtcgacgatc tggcgggcgg cggcttcgtc caggcccaag gtgtcgatga
     4921 gccagtcgat tgcgcgttgg aggcgcgcga gcatgacttc ggtgttggtt tcggagtcgg
     4981 agtcggagtc ggtgttggtg tcggagtcgg tgtcggtatc ggtatcggcc tgcttcccgt
     5041 cgagcaggcg cccgatctgc tcacgcaaac gcgccacgcc gaacgacagt tcatcgctgc
     5101 gccccggtgc ctcgccgagc cagaacggaa tattcggcgg ctgcccctgc gcgtcctcga
     5161 cccgcacgcg gccgctctcg atacgcagaa tccggtacga cgcattgccg agctgaaaca
     5221 cgtcgccggc cagactttcg accgcgaagt cttcgttgac agtgccgatg ttgattgcct
     5281 gcggttcgag caccaccgca tagtccgcgt tctcgggaat cgtgccgccc gaggtgacgg
     5341 cgaccagctt gccgccgcgc cggccgcgca aggtgccgct caccacgtcg cgatggatat
     5401 aggcgccacg cggaccgttg cggctcgtgt agccttcggc gagcacgcgc agcacggcgt
     5461 cgtactgctc gcgctcgagc tccgcgtaag gcgcggcgcg ccgcatcatg tcgaacag
//
LOCUS       DATASH010000672         4468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_7033975,
            whole genome shotgun sequence.
ACCESSION   DATASH010000672 DATASH010000000
VERSION     DATASH010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4468
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7033975"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VHV99_18040"
     CDS             complement(<1..300)
                     /locus_tag="VHV99_18040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX3637347.1"
                     /translation="MNLRRVLLLVGAAALLGACARHDDSAAAMQAAAALGPQATAADP
                     LARGRYLVKAADCAACHTTADGAPFAGGVKLASPFGTFYGTNITPDKDHGIGNWNA"
     gene            complement(312..1097)
                     /locus_tag="VHV99_18045"
     CDS             complement(312..1097)
                     /locus_tag="VHV99_18045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX3637348.1"
                     /translation="MTAPNQTSAHAPNAPNGAHAASGAARGNVASGVVSGASGAAAAN
                     AASGSLASGAARASAPAAAVVERPLTHFRTLPVSVKVNGEIVGPTDVPAGLMMIDFLH
                     EYLHLTGSRLGCGQGICHACVVIVDKPDGTSEEVRTCITGANFFHGKTIRTIEGHARR
                     NEAGEVVELSPIQQKFLEHFSFQCGYCTPGFVNAATVLIERLKRQPISRDQVEQTITQ
                     ALNDHICRCTGYVRYYEAVKEVVLTTPGLVKEGSPRGRLGGTE"
     gene            complement(1094..3922)
                     /locus_tag="VHV99_18050"
     CDS             complement(1094..3922)
                     /locus_tag="VHV99_18050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX3637349.1"
                     /translation="MGKLDLSRRSFLKASVLAGVSVYIAPMGSRAFAALFEEKILTPV
                     QWDAANGQAKFRIDGIAKVTGCKVFARDIRAADMPHWPQQQSHAFILRTTQADRSYEG
                     FDLALLGDDLKPDRVVTAEDLTRDGLIFPAFYGDDMLLPPGKTPAYLGQAVAILIYHD
                     FARFRFAKDKLKFQDQIIRYGAQTGPLERDPWGTFRFVRVGGKTPYDDDTFSSLKDAP
                     VFPSMMRKHQPVWPDGVEHGKLDEQGMFHAGQIQQELDHPSADWLVMEREYTTQSIDT
                     AALEPDNANCWYDTATQSLHMVVPTQAPLEVVESAAEMVAKCAFPVKNLFVHPCYTVG
                     YGSKDHFNVPFYGLVCAMYADGRPVRLANDRYEQFQTSLKRHAFKMNYRIAVDRNTGL
                     LQSFKAAMEANGGGRCNFSPSVAMVGATAAQSIYYFPKNDLSAVAIASRAIDAGSARG
                     YGTLQSMAATEMMVDEIAAQLNLDPIDFRLKNALRSGMKNTQGAVPAGAIRVDEVLQQ
                     AKLHPLWMNRAKRKAEFEAAHPGKRYGVGFACVQKDFGTGAETSFAKVEFGADGKISL
                     QHTAAEIGTGVSTSQAIAVAKWLGKPATDVHVAITDWPDLPVVTSGDPYMMSQAEQDK
                     LAANPRWSPGYASPSSATNSAFYFTHSTREAARVVFAHGLWPAAQAIWRQGGGGGQAA
                     PLVVRIEDARWVDGKLSAAGLEALPFEQLAKKAHELGLVTGATVHVFNRWQWTEADFE
                     IDGSVERLPLDGLAVRYGDSASADKQKLQTTSNRYRVLDRQRVFIPPVQRNNAAVTYY
                     SAVGTLVELSVHEASGKVDLLAHHSIMECGNQLSPQLVSGQLQGGLAMGIGHALHEYL
                     PLYEDGPGNGTWNFNRYHLPRATDVAVWTQTGEVLPPLTESDPPKGIAEVVMIPVVGA
                     IVNGIAHAIDHRFTDLPVTPQRIQEVLA"
BASE COUNT          804 a         1414 c         1390 g          860 t
ORIGIN      
        1 cgcattccag ttgccgatac cgtggtcctt gtccggcgtg atgttggtgc cgtagaaggt
       61 gccgaacggc gaggccagct tcacgccgcc ggcgaacggc gcgccgtcgg cggtggtgtg
      121 gcaggcggca cagtcggcgg ccttgacgag ataacgtccg cgcgcgagcg gatcggcggc
      181 ggtggcttgc ggtcccaacg cggcagcagc ctgcatcgcg gcagcggagt cgtcgtgtct
      241 agcgcaagcg cccagtaaag cagcggcgcc gaccagcagc aacacgcggc gcagattcat
      301 cgaacggctc atcattcggt gccccccaat cgcccacggg gactgccttc ttttacgagg
      361 ccgggcgtcg tcagcacgac ttccttcacg gcttcgtaat agcgcacgta gcccgtgcaa
      421 cggcagatat ggtcgttcag cgcctgcgtg atggtttgct cgacctggtc cctcgagatc
      481 ggctgacgct tcagacgttc gatcagcaca gtcgccgcat tgacgaaacc gggcgtgcag
      541 tagccacact gaaaactgaa gtgttcaaga aacttctgct ggatcggcga cagctcgacg
      601 acctcgcccg cctcgttgcg cctggcgtgc ccttcgatcg tgcgaatggt tttgccgtgg
      661 aagaaattcg cgccggtgat gcaggtgcgt acttcttcgc tggtgccgtc cggcttgtcg
      721 acgatcacga cgcacgcgtg gcagatgccc tggccacagc cgaggcgcga gccggtcaga
      781 tgcaggtact cgtgcaggaa gtcgatcatc atcagaccgg cgggcacgtc ggtcgggccg
      841 acgatctcgc cgttcacctt gaccgatacc ggcagcgtgc ggaaatgcgt caacggacgc
      901 tcgaccacgg cagcagccgg tgcgctggcg cgggcagctc ccgaggcgag cgagccggag
      961 gcggcattgg ccgcagcagc acccgaagca ccggacacga cgcccgaggc gacgttgccg
     1021 cgcgccgcgc cacttgcagc atgagcgcca ttaggggcat taggggcatg agcggaagtt
     1081 tgattggggg ctgtcatgcg agcacttcct gaatacgttg cggggtaacc ggcagatcgg
     1141 taaagcgatg atcgattgcg tgggcgatgc cgttcacgat ggcgccgacc accggaatca
     1201 tcaccacctc ggcgatgccc ttcggcggat cgctttcggt cagcggcggc aggacttcgc
     1261 cggtctgggt ccagacggcg acgtcagtcg cgcgcggcaa gtggtagcga ttgaagttcc
     1321 acgtgccgtt gccggggccg tcctcataga gcggcagata ctcgtgcaat gcatggccaa
     1381 tccccatggc cagcccgcct tgcagttggc ccgacacgag ttgcggcgag agctgattgc
     1441 cgcattccat gatcgagtga tgcgcgagca ggtccacctt gccgctcgct tcatgcaccg
     1501 acagttcgac cagcgtgccg accgcgctgt agtacgtgac cgcggcgtta ttgcgttgca
     1561 ccggcggaat gaacacgcgc tgacggtcga gcacgcggta tcgattcgac gtggtctgca
     1621 gtttttgctt atcggccgaa gcgctatcgc cgtagcgcac ggcaagacca tcgagcggca
     1681 aacgttcgac gctgccgtcg atctcgaagt ccgcttcggt ccattgccag cggttgaata
     1741 cgtgcacagt cgcgccggtt accagaccca actcgtgcgc tttcttcgcg agctgttcga
     1801 atggcagcgc ttcgagcccc gctgcggaca gcttgccgtc gacccagcgc gcatcttcga
     1861 tgcgaaccac cagcggcgcg gcctgcccgc caccgccgcc ttgacgccag atcgcctgcg
     1921 ccgccggcca caagccatgc gcgaagacga cgcgggcggc ttcgcgcgtg ctgtgcgtga
     1981 agtagaaggc cgagttcgtc gcgctcgacg gcgacgcata acccggcgac cagcgcgggt
     2041 tcgccgcgag cttgtcctgc tccgcttgcg acatcatgta cggatcgccg ctcgtcacga
     2101 ccggcagatc gggccaatcg gtgattgcga catgcacgtc ggtggccggt ttgccgagcc
     2161 acttcgcgac cgcgattgcc tgcgaggtcg acacgcccgt gccgatttca gccgccgtgt
     2221 gttgcaggct gatcttgccg tcggcgccga actcgacctt cgcgaacgag gtttccgcgc
     2281 cggtgccgaa gtccttctgc acacacgcaa agccgacgcc gtagcgcttg cccggatgcg
     2341 cggcctcgaa ctccgcttta cgtttcgcgc gattcatcca cagcggatgc agcttcgctt
     2401 gctgcaacac ctcgtcgacg cgaatcgcgc ccgcgggcac tgcgccctgg gtgttcttca
     2461 tgcccgaacg cagcgcgttt ttcaggcgga agtcgatcgg atcgagattc agttgcgcgg
     2521 cgatttcgtc gaccatcatc tcggtcgcgg ccatgctttg cagcgtgccg tagccgcgtg
     2581 ccgagcccgc gtcgatcgcg cgcgaagcga ttgccaccgc ggacagatcg ttcttcggga
     2641 agtagtagat cgactgtgcc gccgtcgcgc ccaccatcgc caccgacggc gaaaagttac
     2701 aacgtccgcc accgttcgct tccatcgcgg ccttgaacga ttgcagcaac cctgtgttcc
     2761 tgtcgaccgc gatccggtag ttcatcttga acgcgtggcg tttcaaggac gtctggaact
     2821 gctcatagcg atcgttagcc aaccgcaccg ggcggccatc cgcatacatc gcgcagacga
     2881 gaccgtagaa cggcacgttg aagtggtcct tcgaaccgta gccgaccgta tagcatgggt
     2941 gcacaaacag attcttcacc gggaacgcgc atttcgcgac catctctgcc gcgctttcca
     3001 cgacttccag cggcgcctgg gtcggcacga ccatgtgcaa cgattgcgtc gccgtgtcgt
     3061 accagcagtt cgcgttgtcg ggttcgagcg cggccgtgtc gatcgactgc gtggtgtact
     3121 cgcgctccat caccagccag tccgccgaag gatgatccag ttcctgctgg atctgcccgg
     3181 cgtggaacat gccctgctcg tcgagcttgc cgtgttcgac gccgtccggc cacaccggct
     3241 ggtgcttgcg catcatgctc gggaacaccg gcgcgtcttt cagactggag aatgtgtcgt
     3301 cgtcatacgg cgtcttgccg ccgacgcgca cgaagcggaa tgtgccccac ggatcgcgtt
     3361 cgagcggccc ggtctgcgcg ccgtaacgga tgatctgatc ctggaacttg agcttgtctt
     3421 tcgcgaaccg gaagcgcgcg aagtcgtgat agatcaggat cgccactgcc tggccgagat
     3481 acgcgggcgt cttgcccggc ggcagcagca tgtcgtcgcc gtagaaggcc gggaagatca
     3541 gaccatcgcg cgtcagatct tcagccgtga ccacgcgatc gggcttcaga tcgtcgccga
     3601 gcagcgccag gtcgaagcct tcatagctgc ggtcagcttg cgtcgtgcgc aaaatgaagg
     3661 cgtgcgactg ctgctgtggc cagtgcggca tgtcggcggc gcggatgtca cgcgcgaaca
     3721 ctttgcaacc ggtcactttc gcgatcccgt cgatgcggaa tttcgcctgg ccgttagccg
     3781 catcccattg aaccggcgtc aggatttttt cttcgaacaa cgcagcgaat gcgcggctgc
     3841 ccatcggcgc gatatacacc gacacacccg ccaatacgct ggccttcaga aaactacgac
     3901 gcgaaaggtc gagtttcccc atggacttct cctttagtga acacagcggc aactcgcggg
     3961 caggacgacc gcggaaagcc agtcgatagc tcgtgaaaaa catcacactt cgtgcgccgc
     4021 gcacgcgatt gcgctcgcac acgcgccgaa cggctgtcac cacgagggag gtgttcgatc
     4081 cgcgggcgct tgcatacgcc cgggcgtacc ggcgacgtgc agcggccatc acttcggctg
     4141 tgccgcagtc gatctgattt accttgaact accgtgagtt gcctttttcc ctcagtccta
     4201 ctgagggcaa ggggcgggat tatctcgctg tttggacgca cggtgcgtca ttttttgtca
     4261 gctatctcgc aaataacgga tcgggtatga atcgtcatgg cggcgggttt atcgcgcatt
     4321 tcagactgat cccacgcaac attgagaaag ggacgatatg ttgaagaagg tcaaaaaaat
     4381 atccttacgg gtccctttgg agactgcctg tctcgccgta aaagcatggc aagtctgtga
     4441 acgggaaaac catttccgtc agtatatt
//
LOCUS       DATASH010000673         1824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1100567,
            whole genome shotgun sequence.
ACCESSION   DATASH010000673 DATASH010000000
VERSION     DATASH010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1100567"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="VHV99_18055"
     CDS             <1..745
                     /locus_tag="VHV99_18055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013341532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX3637350.1"
                     /translation="QQRTRHEVQRQLDLVRQVGAHSPDTRMRFCVHEADRRALDTQLS
                     ARGIDPAAGWLVLHPGATAASRRYPPERYGDVATRLVRETGASLLITGNSSERPLAEA
                     VIRAAARGVRTHLHDLSGTLTLGELAALIERASVLISNNSGPVHLASALGAPVVDLYA
                     LTNPQHAPWQTPHRVLFRDVECRWCYRSVCPQQHHACLLGVAPGEVVQAALELRAAMP
                     MAEANPKPDPNGNASHADAPPLADEPLVP"
     gene            769..>1824
                     /locus_tag="VHV99_18060"
     CDS             769..>1824
                     /locus_tag="VHV99_18060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018434953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX3637351.1"
                     /translation="MYTLGINAVYHDSAAALLRDGVVLAAAEDERFTHIKHAKRPVPF
                     STWQLPFDAIDYCLKAAGITLADIDHVAYSYDPTLFGGMPSRPGATIELPFDPASTRL
                     TDPSASPWDPLFLSYIANAKGQLLDGAPHHLRKRFQGVHREHDFEWHFVEHHLAHEAS
                     AFLAAPFDDTAVLTMDGRGEGVTTSMGQFIGGEYTRLRQVELPHSLGLLYEAVTDWLG
                     FLHSSDEYKVMALASYGKPAYLDVFREIVRYRADGSYTVEAPRLTARFGPPRERGGPL
                     EQRHFDIAHSLQRVLEETVLQLAGWLHRQTGLRTLSMAGGVALNCVMNSKLRDAGPFE
                     DVWVQPAAGDAGTALGAA"
BASE COUNT          300 a          645 c          573 g          306 t
ORIGIN      
        1 gcagcagcgc acgcgtcatg aagtgcaacg ccagctcgat ctcgtgcgcc aggtcggcgc
       61 acactcgcct gatacgcgca tgcgcttctg cgtgcacgag gccgaccggc gcgcgctcga
      121 cacgcaactg tcggcgcgcg gcatcgatcc cgcggccgga tggctcgtgc tgcatccggg
      181 agcgaccgct gcgtcgcgcc gctatccgcc tgaacgttac ggcgacgtcg ccacgcggct
      241 cgtgcgcgaa accggcgcgt cgttactgat taccggcaat tccagcgaac gtccgctggc
      301 cgaggccgtg atccgcgccg ccgcgcgtgg cgttcgcacg cacctgcacg acctgagcgg
      361 cacgctgacc ctcggcgaac tcgccgcgct gattgaacgc gcgagcgtgc tcatttccaa
      421 caacagcggg cccgtgcatc tggcttccgc gttgggcgcg ccggttgtcg atctgtacgc
      481 gctaaccaat ccgcagcatg cgccctggca gacaccgcat cgcgtgctgt ttcgcgatgt
      541 cgagtgccgc tggtgttatc gcagcgtgtg tccacaacag catcacgcgt gtctgctcgg
      601 cgtcgcgcct ggcgaggtcg tgcaagccgc gctcgaattg cgcgccgcca tgccgatggc
      661 cgaggccaac ccgaagccgg acccgaatgg caacgccagc cacgccgacg cgccgccgct
      721 tgccgacgaa ccgctggttc cctgaaacct ctttctttgc aaacgcccat gtacacactc
      781 ggaatcaatg ccgtctacca cgacagcgcc gcagcgttgc tgcgcgacgg cgtggtcctc
      841 gccgctgcgg aagacgaacg cttcacgcac attaaacatg cgaaacgccc ggtgccgttt
      901 tcgacctggc agttgccatt cgacgcgatc gactattgcc tgaaggcggc cggcatcacg
      961 ctcgccgaca tcgatcacgt ggcttattcg tacgacccca cgctgttcgg cggcatgccc
     1021 agccgtccgg gcgccaccat cgagctgccg ttcgatcccg ccagcacgcg cctgactgac
     1081 ccttccgcat cgccgtggga cccgctgttc ctgagctaca tcgcgaacgc aaaggggcaa
     1141 ttgctcgacg gcgcgccgca tcatttgcgc aagcgttttc agggcgtgca ccgcgagcac
     1201 gacttcgaat ggcacttcgt ggaacatcac ctcgcgcatg aagcgagcgc attcctcgcc
     1261 gcgccgttcg acgacaccgc cgtgctgacc atggacggcc gtggcgaagg cgtgacgacg
     1321 agcatgggac agttcatcgg cggcgaatat acgcggctca ggcaggtcga actgccgcac
     1381 tcgctgggtc tgctctacga ggccgtcacc gactggctcg gcttcctgca ttcgtcggac
     1441 gagtacaagg tgatggcgct cgcgtcatac ggtaagcctg cctacctcga tgtgttccgc
     1501 gagatcgtcc gctatcgcgc cgacggcagc tacaccgtcg aggcaccgcg cctgaccgcg
     1561 cggttcggac cgccgcgcga gcgtggcggt ccgctcgaac agcgccactt cgacatcgcg
     1621 cattcgctgc agcgcgtgct cgaagaaacc gtgctgcaac tggccggctg gctgcatcgg
     1681 caaacggggc tgcgcaccct cagcatggcc ggcggcgtcg cgctgaactg cgtgatgaat
     1741 tcgaagctgc gcgatgccgg gccgttcgag gacgtctggg tccaacccgc ggccggcgat
     1801 gcgggcaccg cgctcggcgc ggcg
//
LOCUS       DATASH010000674         2650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3471987,
            whole genome shotgun sequence.
ACCESSION   DATASH010000674 DATASH010000000
VERSION     DATASH010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3471987"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(276..1445)
                     /locus_tag="VHV99_18065"
     CDS             complement(276..1445)
                     /locus_tag="VHV99_18065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007742209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03118 family protein"
                     /protein_id="HEX3637352.1"
                     /translation="MKSLFVVFGFVACGAAIATLVSCGGGGSSSNNSGTSSPSSFTAT
                     ALVSNGAVSAPHTDPNLKNGWGVAFNPKGFVWVADNGTNVATLYDGNGVPQSLVVTIP
                     NGTHGEAAPTGIVFNGTQSFTVTQNGTSGVAAFIFAGEGGTITAWAPSVGPTNAFTMY
                     DDGTGGAVYKGLALAANNGNNFLYATDFHNNKIDVFDANFTKVTMPGGFKDPAIPAGF
                     APFGIQLIGSNLFVTYAQQNAAKHDDVAGTGLGMVDVFDTGGNLKQHFATGTPLNAPW
                     GIAQAPGNFGPFSGAILIGNFGDGTINAFDASSGRSMGPLNSSNGSAIIEHGLWGIAF
                     GNDLNNQPSNTLFFAAGPNDEADGVYGRINLNTTSNSNNGSSTGTSSSGGSSVGM"
     gene            complement(1612..2145)
                     /locus_tag="VHV99_18070"
     CDS             complement(1612..2145)
                     /locus_tag="VHV99_18070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004552423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX3637353.1"
                     /translation="MTGKVRLQLGAKTRDLAMFNLAIDSKLRACDLTKLRVGDVCLGS
                     RVAPRAAVMQQKTQRPVQFGITEQTRESVETWIKAAELTPSDFLFPGRIHTSPHFSTR
                     QYARLVHRWVRSVGLDDTAHGTHTMRRTKASLIYRRTKNLRAVQLLLGHTKLESTVRY
                     LGIGADDALEMAEQTEV"
     gene            2346..>2650
                     /locus_tag="VHV99_18075"
     CDS             2346..>2650
                     /locus_tag="VHV99_18075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007733626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX3637354.1"
                     /translation="MVTTGKIALCATLFVIAVSLIEACVLTRRQRNSAMPFDWYEVWI
                     SLADVAGRKLLALLPLSLATPVFALAWDHRIFTVTLNSALMVFLLFIGQEFCYYWYHR
                     "
BASE COUNT          494 a          789 c          774 g          593 t
ORIGIN      
        1 gccctcagtc attgccggct tcccggcctc ttcacgcaat tcctatctgt agacagcctt
       61 gggaatttga tgcatgtatg cctccattcc taaattctac ggaatgttgg cgtctacttt
      121 ctccgaccga cctcagatga ggcagtcaca agcatggcag atcgatccgc acgcagcggc
      181 gcttgccgca acacgggtaa ggtctataca gctctctcac gaaagaacgg ccgctctttg
      241 tgcgagtgcg gccgttctcg ctagctgaag acgatctaca tgccgacgct cgaaccgccg
      301 ctcgagctcg tgccggtgct cgatccgttg ttcgagttcg acgtcgtatt cagattgatt
      361 cttccataga cgccgtccgc ttcgtcgttg gggcccgctg cgaagaagag cgtattggag
      421 ggctggttgt tgaggtcgtt gccgaacgcg atgccccaca aaccgtgctc gatgatcgcg
      481 ctgccgttcg acgaattgag cgggcccatc gaccggccac tcgatgcatc gaatgcgttg
      541 atcgtcccat cgccgaaatt accgatcaga atcgcgccac tgaacgggcc gaaattgccc
      601 ggcgcctgcg caatacccca cggcgcgttc aacggcgtgc cggtcgcgaa gtgctgcttg
      661 agattgcccc ccgtatcgaa cacgtcgacc atgcctagcc ccgtgcctgc gacgtcgtca
      721 tgcttcgccg cattttgctg tgcgtaggtg acgaagaggt tcgagccgat caactggatg
      781 ccgaagggcg cgaatccggc gggaatggcc gggtccttga agccgcctgg catcgtgacc
      841 ttggtgaagt tggcatcgaa gacgtcgatc ttgttgttat ggaagtcggt ggcatagagg
      901 aaattgttgc cgttgttggc cgcaagcgcg agccccttgt acacggcgcc gcccgtgccg
      961 tcgtcgtaca ttgtgaatgc gttggttggc ccgacagacg gcgcccatgc ggtgatggtg
     1021 ccgccttcgc ccgcgaagat gaacgcggca acgccagacg ttccgttttg cgtcacggta
     1081 aagctttgcg tgccgttgaa cacgatgccc gttggcgccg cctcgccgtg tgtgccgttg
     1141 gggatcgtga ccaccagcga ttgcggaacg ccgttgccgt catagagcgt ggcgacattg
     1201 gtgccgttgt ccgcgaccca gacgaatcct ttcggattaa aggcgacgcc ccatccgttc
     1261 ttcaaattcg ggtcggtgtg tggcgcggag accgcaccat tagagacgag cgccgtcgcc
     1321 gtgaacgacg aaggcgaaga cgtgcctgaa ttgttcgaac tggaaccacc tccgccgcat
     1381 gacacgagag tcgcgatagc cgcgccgcat gctacaaagc cgaacaccac gaatagcgac
     1441 ttcatgacgc actccttgtt ataaaggttg gaacaagaga acgaacaagg ggcggggttt
     1501 attccagcgg tgggggctgt tttgctcgtc gatgttcatc cgtttcctgc gaaatgcagt
     1561 tcgcaggaaa cgtgcgagaa gacggaatac tcgtatgcgt cagcctgccg ctcagacttc
     1621 ggtttgttcg gccatctcga gcgcgtcgtc ggctccgatg ccgaggtatc gcactgtgct
     1681 ctcaagtttg gtatggccga gcagcagttg tacggcccgg agatttttcg tacggcgata
     1741 gatcagcgat gctttggtcc ggcgcatcgt gtgcgttcca tgcgcggtgt cgtcgagtcc
     1801 gacggaacgt acccagcggt gcacgaggcg tgcgtactgc cgcgttgaaa aatgaggcga
     1861 cgtgtggatg cgacccggga aaagaaagtc tgacggtgtc agctcggccg cttttatcca
     1921 cgtttccacg ctttcgcgtg tttgctcagt tatcccgaac tgcaccggtc gctgcgtctt
     1981 ctgctgcatg acggccgccc gcggcgccac gcgagagccg aggcagacgt cacccacccg
     2041 cagtttcgtc aggtcgcacg cccgtagctt gctgtcgatc gccagattga acatcgcgag
     2101 atcacgtgtt ttagccccaa gctgcagccg caccttgcct gtcactgaag caagcaatag
     2161 aaaccgttag cctgtcgcag tccatcaccg cggcttgccc gtcacgggcg gaatttcgcg
     2221 acatctggcg acatagttta cgcatggttt ttaaccggac cgcacattac attagcttca
     2281 cccgacgcga tagcggcctt ttcccgcaga cgatgcgtcc aatctcaacc ggttatctgc
     2341 ccatcatggt taccactgga aaaatcgctc tttgcgctac gttgttcgtc atcgccgtct
     2401 cgctgatcga ggcatgcgtg ctcacccgca gacagcgcaa tagcgcaatg cctttcgact
     2461 ggtatgaagt gtggatttcg ctggccgatg tggccggtcg caagctgctc gcactgttgc
     2521 cgctttcgct cgcgacgccg gttttcgcgc ttgcctggga tcaccggatc ttcaccgtca
     2581 cgctcaacag cgcgctgatg gtgttcctgc tctttatcgg ccaggagttc tgctactact
     2641 ggtaccaccg
//
LOCUS       DATASH010000675         5798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5252020,
            whole genome shotgun sequence.
ACCESSION   DATASH010000675 DATASH010000000
VERSION     DATASH010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5798
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5252020"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..1758)
                     /locus_tag="VHV99_18080"
     CDS             complement(70..1758)
                     /locus_tag="VHV99_18080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase"
                     /protein_id="HEX3637355.1"
                     /translation="MSHTDDPRDESPASDLPHDPVRRRLLAAGVGLAGLTGLSLSGCN
                     LFDSRPATPKTAEDIALDKTLQARVRHIVVIYAENRSFSNLWGNYPGVQYPLEAVPAS
                     RYVQLDRDGRTPMPVLPKIWGGLVPQAQEVDGKRYMISEKQIDGVPNGPFHLVDTEGR
                     PLPNGVITRDLVHRFYQNQMQINAGRNNQFAAWGDSGGLVMGHYRNSAQSLKLWNIAR
                     QNVLCDNFFMAAFGGSWLNHIYLIAAQAPFYPDAQNGPAKKLLSVVDGDDPLGTRLKL
                     APDSPASALDGPPSYVSDGALTADGYAVNTMAPPYQPSNVRPAEGGDPALADPSNYRV
                     LPAQTYATIGDRLSDKGVDWAWYSGGWDYALTHRDTGMVPDFQYHHQPFNYFRSFAPG
                     TQTRTQHLRDAGLGDDPSTNKLIADIDAGRLPAVTFYKPQGNLNMHAGYADVESGDRH
                     LANVVERIQRGPQWESTVVIITHDENGGWWDHVPPPKGDRWGPGSRIPALVIAPFAKK
                     GVVDHTVYDTNSILRFITRVHDLAPLDGVVARDRAFAANGEAPLGDMTATLDLA"
     gene            complement(2153..2971)
                     /locus_tag="VHV99_18085"
     CDS             complement(2153..2971)
                     /locus_tag="VHV99_18085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007589173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX3637356.1"
                     /translation="MSFPHIDLLYSASGLAVGFLVGLTGVGGGSLMTPLLVLLFGIHP
                     ATAVGTDLLYAAATKTAGTLVHGIKGSVDWRITGRLAAGSIPAAAITLVLLNRYGLNT
                     PAASRVIQLVLGYALLVTAVSLVFRPQLARLAYKTGMAREASGKPEAASREARTVAWT
                     VLTGAILGVLVSITSVGAGAIGVTVLLLLYPRLPTARIVGSDIAHAVPLTLIAGTGHW
                     LLGSVNWDLLLSLLVGSLPGIVLGSMLASRAPEVLLRNALAATLVIVGVKLVLS"
     gene            3226..5475
                     /locus_tag="VHV99_18090"
                     /pseudo
     CDS             3226..5475
                     /locus_tag="VHV99_18090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013428882.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
     gene            complement(5507..>5798)
                     /locus_tag="VHV99_18095"
     CDS             complement(5507..>5798)
                     /locus_tag="VHV99_18095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008353390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX3637357.1"
                     /translation="AKGMNLAVSDVRVLTAALQAFYEEGASGKLDRYTETALKRVWRA
                     EHFSWWMTRMLHTLDDTSPFEQRLQVAELEHVTTSRAAATALAENYVGSVAV"
BASE COUNT         1073 a         1768 c         1877 g         1080 t
ORIGIN      
        1 cgatgcgcct caggcgtttg caggtcaggc attttccgct cgggcgtttg cgactccggc
       61 attcgcgatt caggcgagat cgagcgtagc cgtcatgtcc cctagcggcg cttcaccgtt
      121 tgccgcgaat gcccggtccc gcgcgacaac gccgtcaagt ggcgccaggt cgtggacgcg
      181 cgtgatgaag cgcaggatgg agttggtgtc gtagaccgta tggtcgacga cacctttttt
      241 cgcgaacggc gcaatcacca gggccggaat gcgcgacccc ggtccccatc gatccccttt
      301 cggcggcggc acgtgatccc accagccgcc gttctcgtcg tgcgtgatga tcacgaccgt
      361 gctttcccat tgcggcccgc gctggattcg ctcgactacg ttggcgagat gccggtcacc
      421 cgattcgaca tcggcgtaac ccgcatgcat attcaaattg ccttgcggtt tatagaaggt
      481 cacagccggg aggcgcccag cgtcaatatc cgcaatgagt ttgttcgtcg acggatcgtc
      541 gcccagaccc gcgtcgcgca gatgttgcgt gcgagtctgc gtgccgggag cgaagctgcg
      601 gaaataattg aacggttgat ggtgatactg gaaatcggga accatgccgg tgtcgcgatg
      661 cgtgagcgca tagtcccagc cgccgctata ccacgcccag tccacgcctt tgtccgataa
      721 ccggtcgcca atggtggcgt aagtctgcgc gggcagcacg cgatagttcg atgggtccgc
      781 cagcgccgga tcgccgcctt ccgccgggcg gacgttgctt ggctgatacg gtggcgccat
      841 tgtgttgacg gcgtagccgt cggctgtgag cgcgccgtcg ctgacgtagg acggggggcc
      901 gtccagcgcc gatgccggcg aatcaggcgc gagtttcagc cgcgtgccaa gcggatcgtc
      961 cccgtccaca acggacagga gctttttcgc cgggccgttc tgtgcgtccg gataaaaagg
     1021 ggcttgcgcc gcaatcaggt aaatatgatt gagccaggac ccaccgaacg cggccatgaa
     1081 gaagttgtcg cacagcacgt tttgccgggc gatgttccaa agcttcaacg attgcgctga
     1141 attgcggtaa tgccccatga ccaggccgcc ggaatctccc catgcggcga actggttatt
     1201 gcggcccgcg ttgatctgca tctggttctg atagaaacgg tgcacgaggt cgcgcgtaat
     1261 cacgccgttc ggcagcggcc gtccctctgt gtcgacaaga tgaaacggtc cgttcggcac
     1321 gccgtcaatc tgtttctctg aaatcatgta gcgcttgccg tccacttcct gcgcttgcgg
     1381 cacgaggccg ccccagattt tcggcaggac cggcatgggt gtgcgcccgt cgcggtctag
     1441 ctggacgtag cgcgacgcag gcactgcttc cagcggatat tgcacgccag ggtagttacc
     1501 ccagaggttg ctgaaactgc gattttccgc gtagatcacg acaatatgcc ggacccgcgc
     1561 ttgcagcgtt ttgtcgagcg cgatgtcctc ggccgtcttg ggggtagccg ggcgtgagtc
     1621 aaaaagattg cagccgctga gcgacaagcc ggtcaggcca gccaggccga ccccagccgc
     1681 aagcaggcga cggcgcacgg gatcgtgggg caggtcggac gcgggtgatt cgtcgcgggg
     1741 atcgtcagtg tggctcatcg cagctcctgg atgtctggcg gatcatcaat gtgcttgttg
     1801 atgtggctaa caatgtgtgg ctaaacagta tgcgtattga cgtggctaaa taaaatgact
     1861 caccaatgtg acccagtttt gccgcggact attttgaatc aaacccgcga atcaaaacaa
     1921 tgcacaagca cgatgcgacc gggtcgccat ggcgaagcgc gcctgtgcgt tgcagcccgt
     1981 gggttgctta agcacctttc gtctacctga atcttgcccc gaaacgcgtg cttcccgata
     2041 gctgaccgaa cggcctatgc acccaaaaac ggcccggcca gaactcgtat gccgtttggt
     2101 cgactggaaa gcgtcacgct tggcgactac tatcgttcga actgcccgag aatcaactga
     2161 gtaccagttt cacgccgaca atcaccaggg tggcggccag cgcgttgcgc agcagcacct
     2221 ccggcgcccg ggaggcgagc atgctgccca gcacgatccc tggcagcgaa cccacgagca
     2281 gggacagcag caggtcccag ttgacggaac cgagcagcca gtggccggtt cccgcaatta
     2341 gcgtaagtgg aaccgcatgc gcaatatccg acccgactat ccgcgcagtc ggcaaacgcg
     2401 ggtagagcag caacagcacg gtcacgccaa tggcgcccgc accgaccgag gtgattgaga
     2461 cgagcacgcc caggattgcg ccggtgagca cggtccaggc cacggtgcgg gcttcacggc
     2521 tcgccgcctc tggtttgccg ctggcttcgc gggccattcc ggtcttgtag gcgaggcgcg
     2581 caagctgcgg gcggaacacg agcgagacgg cggtaaccag cagcgcgtag ccaagcacga
     2641 gctggatcac ccgggaggcg gcgggtgtgt tcaaaccgta ccggttgaga aggaccaggg
     2701 tgattgccgc cgcgggaatg ctgcctgcgg caagccggcc ggtgatacgc cagtccacgg
     2761 accccttgat gccgtggacc agcgtgccgg ctgtcttggt cgcggccgcg tagagcaagt
     2821 ccgtgccgac cgcggtggcc gggtggatgc cgaacaggag cactagcagc ggcgtcatca
     2881 gcgagccgcc gcctacgcca gtcaggccca cgaggaagcc gacggccagg ccggacgctg
     2941 aatacagtaa gtcgatgtga ggaaaagaca tgcggcgggg agaaccggtt ggatgagcgt
     3001 cggtcgaaaa acggtcgatg atagcgggtc cgcggtgcgc ttgcctccgg gtccgcaaaa
     3061 atttgattcg caggctacgg ccgtacaatc ggccgggtgt ccaggcaact ctttcaggaa
     3121 ggtgtaatgg aaacgaagga acaacagcaa gcaaagcagg cgctcagcga ccgacaggcc
     3181 agtgatatcg ctcgcgaagc tgcccatggt cccgctcatg acgacatgcc cgacccgatc
     3241 cgcggtacgg ccggcgttct cgaggttcct ccgtcgacgc ccgtcacaaa cacggacggc
     3301 gcgtttatcg caactgaaca tacgaactac gcgaacgagc gtcagtttgt gctcaaggcc
     3361 ggcaatacct tcgtggtcaa cgacgcgaat ggcgacatcc gcggcaatga cgacggtcta
     3421 ttcgtgaatg acacccgtgt gttgtcacag ttgcgcttaa ccttcggcgg ccgctcgccg
     3481 tcgctgctgt ccggcagcgt gtcgagcgac aacgccgtgt ttaccgcgca tttgaccaac
     3541 cgcccgctgc cgctcattgg cgaggcgagc acgcccgaag gtgtgatcca cgtggagcgc
     3601 cgccgggtgt tgtcgcggca cgtgctgcac gaagccattg tgttgaccaa ttacggcacg
     3661 gaaccggcca ctgtgccgct ttcgatcaac ttcgccgccg acttccacga catgttcgag
     3721 gtgcgcggct ccacccgccc gaaacgcgga accatgcgcg agccgtgcat cgaagatggc
     3781 gaggtcgtgt tgcgctatac ggggctggat gaagtcgaga ggaccgcgcg tatccagttt
     3841 tcgccggcgc cgcattcact ttcggtcaat cgtgcggatt acgtattgcg aatccagtcg
     3901 gaaacggcca tgtcgatcta cttgtcgatc acggccgtca cgcacgcgtt gagcgacgcc
     3961 agcgcggcga gcaagaagat cgcagaggaa ggcgcggaat gtgcgccgga gtcggggcgg
     4021 ccggcgattc gtgaggcgct tgtcgacgcc catcggcgta tgcgcgagcg acggcgttca
     4081 gtcgcgcgcg tgcgctcgag caacccgctg ttcagcgaat ggatcgatcg ttcgcttgcc
     4141 gacctcggtt tgctgacgac tgatcttgaa acgggaccgt atccgtacgc gggcattccg
     4201 tggttttcga cggccttcgg ccgcgacgcc atcattactt cgctgcaaat gctgtggatc
     4261 gatccgtcgc ttgctcgcgg cgtgctgcgc tttctcgcag cgcatcaggc gcgcgaggac
     4321 tccgcatttc gtgacgcgga agtcggcaag atcatgcacg agacccgcaa tagcgaaatg
     4381 gctgcgacgg gcgaagtgcc gtttgcgatg tactacggcg gggtcgacag cacgccgttg
     4441 ttcgtcgcgc tggccggcgc atacgccgag cgaacgggcg acacggcgct gatcgacgaa
     4501 ttgtggcccg ccttgcaact ggcttcgcaa tggatcgcac gtcattgcga caagaacgaa
     4561 ttcggcttgc tgagctacca gcgcgcaacc gaagcggggc ttgccaacca gggctggaaa
     4621 gacagcggcg actccgtgtt tcacgccgac ggcagtttcc cggtcgggcc gatttctctg
     4681 gttgaggtcc aggcctacgc ctccaccgcg ttcgccacca tggcccgctt cgcaaggcag
     4741 cgaaacgcgc cggagcaggc gcaggagttc gagcagcgcg cgcaaaacat ccgcgagcgc
     4801 gtggagacaa tgttctggat gcccgagtcg gagttctacg gcattgcgct cgacggcaag
     4861 ggcgagttgt gcaaggtgct ggcgtcgaat gcgggacact tgctgacctt cgacctcgtc
     4921 gagcgcagcc gcggtgaagc tgtcgcgcgc caacttgagt cgaccttgtt caatactggc
     4981 tggggcgtcc gtacgctggc agcaggccag ccgcgcttta atcctatgtc gtaccacaac
     5041 ggctccgtgt ggccgcacga cagcgccatg tgcgctcgcg gactcgcgcg ctacggcgat
     5101 cgcggcggtg cagtgcgttt gttgcaggca ctattcgaag cggccgtcac cttcgatatg
     5161 cgcctgcccg agttgttctg cggcttcacc cggcaacgcg gcgagcggcc gatcggctat
     5221 ccggtcgcgt gtttgccgca ggcgtgggcg gctggatcgc cgttcatgat cctggaggcc
     5281 tgtctcggga tcacaatcga tgccgagcac taggaagtgc ggatcgaccg gccgcagttg
     5341 cccgatggta tcgactggct cgaaatcggt gatctgcgag tgggcgacaa gaccgtgtcc
     5401 attaccttcc ggcgcgtggg cgggcaggtc gtgccgtcca tcgaaggcga tatccgggtg
     5461 gtggcggtgc tttgaacgca ccggactccg ctaccaccgc caccgcttaa accgcgacgc
     5521 tgccgacgta gttctcggcc agcgccgtgg cagcggcacg cgaggtggtc acgtgttcga
     5581 gctcggccac ttgcaggcgt tgttcgaacg gcgaggtgtc gtcgagtgtg tgcagcatcc
     5641 gggtcatcca ccacgagaaa tgctcggcgc gccagacccg cttcaaggcg gtttcggtgt
     5701 agcggtccag cttgccgctc gcgccttctt catagaacgc ttgcagggcc gcggtcagca
     5761 cacggacgtc ggataccgcc aggttcatgc cttttgcg
//
LOCUS       DATASH010000676         6166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1694427,
            whole genome shotgun sequence.
ACCESSION   DATASH010000676 DATASH010000000
VERSION     DATASH010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6166
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1694427"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /locus_tag="VHV99_18100"
     CDS             <1..1048
                     /locus_tag="VHV99_18100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEX3637358.1"
                     /translation="VTIQSGAGSGASFPDEAFTAAGAEIVDAATAFGADLVLKVQSPT
                     DTELPLLKRGAVLVGMLDPFNTENSSKLAAAGVTAFALEAAPRTTRAQSLDVLSSQAN
                     IAGYKAVLLAATLYPRFMPMLMTAAGTVKAARVLILGAGVAGLQAIATAKRLGAVIEA
                     SDVRPAVKEQIESLGAKFLDVPYETDEEREAAQGVGGYARPMPPSWLTRQSALVHERA
                     KQADIVISTALIPGRAAPTLISVETVQAMKPGSVLVDLAAGRGAEYEGRRGGNCPLSE
                     SDQVVIKHGVTIVGYTNLASMVPADASSLYARNLLDFLKLIITKEGALNIDLADDIVA
                     ATLLARDGEVTRKA"
     gene            1064..1396
                     /locus_tag="VHV99_18105"
     CDS             1064..1396
                     /locus_tag="VHV99_18105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEX3637359.1"
                     /translation="MEVINHTVINLIIFVLAIYVGYHVVWNVTPALHTPLMAVTNAIS
                     AIVIVGAMLAAGLTLGTPGKFFGTLAVALAAVNVFGGFLVTRRMLEMFRKKEPKKLIA
                     DKTAKENA"
     gene            1396..2850
                     /locus_tag="VHV99_18110"
     CDS             1396..2850
                     /locus_tag="VHV99_18110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012327915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEX3637360.1"
                     /translation="MSLNVVTLLYLVASVCFIQALKGLSNPKTARIGNTFGMAGMAIA
                     ILTTIALIIKQANGLGSNLGLGLGLLLVALVIGGAIGAFVAARVEMTKMPELVAAMHS
                     LIGLAAVCIAYAVVSEPSAFGLVDPENTTPGFLPYGNRVELFIGTFVGAITFSGSVIA
                     FGKLSGKYKFRLFQGAPVVYAGQHLINLLLALGMLGFGVIFFLTQAWLPFIIMTIIAF
                     VLGVLIIIPIGGADMPVVVSMLNSYSGWAAAGIGFSLNNPMLIIAGSLVGSSGAILSY
                     IMCRAMNRSFFNVILGGFGNEAGAAAAGGSAEQRPVKSGSADDASFMLGNAETVVIVP
                     GYGLAVARAQHALKELTDKLVEKGIEVKYAIHPVAGRMPGHMNVLLAEAEVPYDMVYE
                     MDDINGEFGQVDVVLVLGANDVVNPAAKNDPKSPIAGMPIIEAYKARTVIVNKRSMAA
                     GYAGLDNDLFYMDKTMMVFGDAKKVIEDMVKAVE"
     gene            complement(3008..3748)
                     /locus_tag="VHV99_18115"
     CDS             complement(3008..3748)
                     /locus_tag="VHV99_18115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HEX3637361.1"
                     /translation="MRRYHRGTVPVLFYQTPTMTRRADRLFQIAELLRGRRLTTAQQL
                     ADWLNISLRTVYRDVRDLQLSGVPIEGEAGIGYRLSRAASLPPLTFTVDELAALAAGA
                     RMLESWGGVGFASGARGALAKIASAMPADKRATLERLAIFAPSFHIKADFSAKVDVLH
                     RAIDARHVVRFAYTDANGASTERRVWPLALAYWGARWTLGAWCELRGDFRNFGLEKIQ
                     TLQVLEQYPDQEGRRLPDLLRHVNAKPR"
     gene            complement(3825..4208)
                     /locus_tag="VHV99_18120"
     CDS             complement(3825..4208)
                     /locus_tag="VHV99_18120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018431914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3637362.1"
                     /translation="MSASSKVVAWFEIPSVDFERAVRFYEAALDFKLSRQEVGGQPIA
                     IFGYEEPATGGAIVHNPSMKPTADGVLVYLNAQPTVDATLARVEQAGGKTDGPVIKLP
                     QDIGYIAFFTDTEGNRLGLHSRTNG"
     gene            complement(4579..5874)
                     /locus_tag="VHV99_18125"
     CDS             complement(4579..5874)
                     /locus_tag="VHV99_18125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEX3637363.1"
                     /translation="MSFDFFLPCPRGLEASLATELAEIAAKHLNGAPFTAGAQVPGGV
                     HFRGGWAAGMAANLHSRLASRVLLKIAHRPYRSEQDVYALAVEQRWEQWFSANETLRV
                     DVTAIKSPLRSLEFTTLRVKDAICDRLREVSGARPNIDTAMPDVRVFAFLTATDCTLY
                     LDTSGDPLFKRGWRLDKGAAPLRENLAAGILRLTGWTAGTPLYDPMCGSGTFLAEAAQ
                     VALNIAPGAERRFGFEKLKQFDAKTWQTLKAAALDAKHAARNSRADLQIFGSDISGDM
                     LDKARANFQRAGLPSIPLKQVDARGMAAPSAEPGILVANPPYGERIEVRGRNARGEIR
                     EGRATREGREDDGFRRAQEETPDSEFFQSLGDALKQRFTGWHAFILTSDRKLPGQLRL
                     RESTKTPLFNGALECRLFRFDLIAGSVRQRPQNNDTPAA"
BASE COUNT         1046 a         1821 c         2088 g         1211 t
ORIGIN      
        1 cgtcacgatc cagagcggcg ccggcagcgg cgcaagcttt cccgacgagg ctttcacagc
       61 cgcaggcgcg gaaatcgtcg atgcggccac cgccttcggc gccgatctcg tcctcaaagt
      121 ccagtccccc accgacaccg aactgccgct gctcaaacgc ggcgctgtac tggtcggcat
      181 gctcgatccg tttaataccg aaaactcatc aaagctggcg gctgcgggcg tgaccgcatt
      241 cgcgctggag gccgcgccac gcaccacgcg tgcgcagagc ctggatgtgt tgagttcaca
      301 ggcgaacatc gccggctaca aggcggtgct gctggcggcc acgctctatc cgcgttttat
      361 gccgatgctg atgaccgccg ccggcaccgt gaaagcggcc cgcgtgctga ttctcggcgc
      421 gggggtggcg gggttgcagg cgattgcgac cgccaagcgc ctgggcgctg tgattgaagc
      481 gtccgacgtg cgcccggcgg tgaaggagca gatcgaatcg cttggcgcca agtttctcga
      541 cgtgccgtac gaaaccgatg aagagcgtga agccgcgcag ggcgtcggtg gctacgcgcg
      601 gccgatgccg ccttcgtggc tgacgcgtca gtctgcattg gtgcatgaac gggccaaaca
      661 ggccgacatc gtgatctcga ccgcattgat tccagggcgg gccgcgccaa cgctgatttc
      721 ggtggaaacc gtgcaggcga tgaaacccgg ctccgtgctg gtggacctcg cggcaggccg
      781 gggcgcggag tacgaaggcc ggcgcggcgg caactgtccg ctcagcgaat cggaccaggt
      841 cgtcatcaaa catggcgtga cgatcgtcgg ctatacgaat ctggcctcga tggtgcccgc
      901 cgacgcgtcg tcgctctacg cacgcaacct gctcgacttc ctcaagctga tcatcacgaa
      961 ggaaggcgcc ctgaacatcg atctcgcgga cgacatcgtc gcggccacgc tgctggcccg
     1021 cgacggagaa gtcacgcgca aggcataagt ggagagtgcg gaaatggaag tcatcaacca
     1081 caccgtcatc aatctgatca tcttcgtgct ggcgatttac gtcggctatc acgttgtctg
     1141 gaacgtcacg cccgcgctgc atacgccgct gatggcggtg accaacgcaa tctcggcgat
     1201 cgtgatcgtc ggcgccatgc tcgccgccgg cctcacgctg ggcacgcccg gcaagttctt
     1261 cggcacgctc gccgtcgcgc tcgccgcagt gaatgtgttc ggcggctttc tcgtcacgag
     1321 gcgaatgctg gagatgttca ggaagaaaga gcccaagaag ctcatcgctg acaagacggc
     1381 caaggagaac gcgtaatgag tctgaacgtc gtcacgttgc tttatctggt cgcgtcggtc
     1441 tgcttcatcc aggcgctcaa ggggctgtcg aatccgaaga cggcgcgcat cggcaatacg
     1501 ttcggcatgg ccgggatggc gattgcgatc ctcaccacca tcgcgttgat catcaagcag
     1561 gctaacgggc ttggctcgaa tctcgggtta gggctcggcc tgctgctggt cgccttggta
     1621 atcggcggcg cgatcggtgc gtttgtcgcc gcgcgcgtcg agatgaccaa gatgccggaa
     1681 ctggtcgcgg cgatgcactc gctgatcggt ctggcagccg tgtgtatcgc gtatgcggtg
     1741 gtgtcggagc cgtccgcgtt cggtctggtc gatccggaga acacaacgcc tggtttcctg
     1801 ccttatggca atcgcgtcga gctgttcatc ggtacgtttg tcggcgccat tacgttctcc
     1861 ggttcggtga tcgcgttcgg caagctatcg ggcaagtaca agttccggct gttccagggt
     1921 gcgccggtcg tctatgcggg gcagcacctg atcaacctgc tgctcgcact cgggatgctc
     1981 ggcttcggcg tgatcttctt cctcacgcag gcgtggttgc cgttcatcat catgacgatc
     2041 atcgcgttcg tgctcggcgt gctgatcatc attccgatcg gcggcgcgga tatgccggtg
     2101 gtcgtgtcga tgctcaactc gtactcgggt tgggcggcgg cgggcatcgg cttctcgctg
     2161 aacaatccga tgctgatcat cgcgggctcg ctggtgggtt cgtcgggtgc gatcctgtcg
     2221 tacatcatgt gccgggcgat gaaccgctcg ttcttcaacg tgattctggg cggcttcggt
     2281 aacgaagcgg gtgcggcggc tgcgggtggt tcggcggagc agcgtccggt gaaatcgggt
     2341 tccgccgacg atgcgtcgtt catgctcggc aatgctgaaa ccgtggtgat cgtgccgggt
     2401 tacggacttg ccgtggcgcg cgcgcagcat gcgttgaagg agttgaccga caagctggtc
     2461 gagaagggca tcgaggtgaa gtacgcgatt cacccggtgg ccggccgcat gccggggcac
     2521 atgaacgtgc tgctcgcgga agccgaagtg ccgtacgaca tggtctacga aatggacgac
     2581 atcaacggtg agttcggcca ggtcgacgtg gtgctggtac tcggtgcgaa cgacgtggtg
     2641 aacccggcgg cgaagaacga tccgaagtcg ccgatcgcgg gcatgccgat catcgaggca
     2701 tacaaggcgc gcacagtcat cgtgaacaaa cggtcgatgg ccgccggtta cgcgggtctc
     2761 gacaacgacc tgttctacat ggacaagacg atgatggtgt tcggcgatgc gaagaaggtg
     2821 atcgaggata tggtgaaggc ggtggagtag cgtgccacta tgcgacgcgg cctgccctgc
     2881 ggcgggccgc agcctcgatg tgcgcgcgac ctctggtgag gcagggcgct tcctgattca
     2941 tagccccgac gcgtactacc gaatctcaca acgcaaatgt ggccgcgtcg caaaatcgcg
     3001 tcgctcttca cctcggcttc gcattcacat gccgcagcaa gtctggcaac cgcctgcctt
     3061 cctgatccgg atactgttcg agcacctgaa gagtctgaat cttctccaga ccgaaattgc
     3121 ggaagtcgcc ccgcaattca caccacgcgc cgagcgtcca gcgcgcgccc caatacgcta
     3181 gcgccagcgg ccatacgcgc cgttcggtag acgcgccgtt cgcatccgta taggcaaagc
     3241 gcaccacgtg acgtgcgtcg atcgctcgat gcagcacgtc caccttggct gaaaaatccg
     3301 ctttgatgtg aaacgacggc gcgaagatcg ccagacgttc gagcgtcgcg cgcttgtcgg
     3361 cgggcattgc cgaagcgatc tttgccaacg cgccgcgcgc gccgcttgcg aagccgaccc
     3421 cgccccacga ttcgagcatc cgcgcgcctg ctgcgagcgc ggccagttca tcgacggtga
     3481 aagtgagcgg cggcaggctc gccgcgcggc tcaaccgata gccgatgccc gcttcgcctt
     3541 cgatcggcac gcctgacaat tgcagatcgc gtacgtcgcg atagacggtg cgcagcgaga
     3601 tattcaacca gtcggcgagt tgttgcgcgg tggtcagacg ccgtccgcgc agcagttcgg
     3661 cgatctggaa caggcggtcg gcgcggcgcg tcatcgtggg cgtctggtag aaaaggacgg
     3721 ggacagtccc gcgatgatag cgccgcatgc agcgctccgg tcgtcgttct gcgcggcgcc
     3781 gttgggccgc cgtgctggtg cgccgcgttg cgtggcgtcg cgtgttagcc gttggtccgc
     3841 gaatgcaggc cgagacggtt gccttccgtg tcggtgaaga acgcgatgta gccgatgtcc
     3901 tgtggcagct tgatcaccgg accatctgtc ttgccgccgg cctgctcgac gcgggcgagg
     3961 gtggcgtcga ccgtcggctg tgcgttcaga tagacgagca cgccgtcggc ggttggtttc
     4021 atcgacgggt tatggacgat cgcgccgccg gtggcgggtt cctcgtagcc gaagatggcg
     4081 atcggctggc cgccgacttc ctggcgactc agtttgaaat cgagcgcggc ttcgtaaaag
     4141 cgaaccgcgc gctcgaaatc gacggacgga atctcgaacc atgcaacaac tttggatgat
     4201 gcggacatga cactctcctg tggatggcgg taagccgtgc aaagcgccgg atggcgtgag
     4261 aaggagtgtg cgggggtgct actgacagcg tagtgtcagt aggacttggc gggggagcaa
     4321 aaggacgtgg tgggcgatga gcgcgtgggg gcgccggtgg tgatggcggg gtgcagagat
     4381 ggcttggcgg gcgcgaacat aggagcggag aagccggggt ggcagcaggg gtggccgacg
     4441 ccggccgatc gccgaggtac tggcaaaaaa accgcgaacc tggatccgcg gtttatttcg
     4501 atgctgcttt gctttgcttt gctttgcttt gctttgcttt gctttgcttt gctttgcttt
     4561 gctttgcttt ccgcctgctt aggccgctgg cgtgtcgttg ttctgcggcc gctggcgcac
     4621 gctgccggcg atcagatcaa agcggaacag ccggcattcg agcgcgccat tgaagagcgg
     4681 cgtcttggtc gattcacgca gacgtaactg gccaggcaac ttgcgatccg acgtcaggat
     4741 gaacgcgtgc cagccagtga agcgctgctt cagtgcatcg ccaagcgatt ggaaaaattc
     4801 gctatccggc gtctcttcct gagcgcggcg gaagccgtcg tcttcgcggc cttcacgcgt
     4861 ggcgcgtcct tcgcggattt cgccgcgcgc gttgcggccg cgcacttcga tccgttcgcc
     4921 atacggcgga tttgcgacga ggatgcccgg ctcagccgac ggcgccgcca tgccacgcgc
     4981 atcgacctgc ttgagcggaa ttgacggcaa gccggcgcgc tggaaattcg cgcgcgcctt
     5041 gtcaagcatg tcgccggaga tatcgctgcc gaagatttgc agatcggcgc gtgaattgcg
     5101 ggcggcgtgc ttggcgtcga gcgcggcggc cttcagcgtt tgccaggtct tggcgtcgaa
     5161 ctgtttgagc ttttcgaagc cgaagcggcg ttccgcgccc ggcgcgatat tcagtgccac
     5221 ttgcgcggct tccgcgagga aggtgccgct gccgcacatc gggtcgtaca gcggcgtgcc
     5281 ggcggtccag ccagtcagac gcaggatgcc tgccgcgagg ttctcgcgaa gcggcgccgc
     5341 gcccttgtcc aggcgccagc cgcgcttgaa gagcggatcg ccggaggtgt cgaggtagag
     5401 cgtgcaatcg gtggcggtga ggaatgcgaa cacgcgcacg tccggcatgg cggtgtcgat
     5461 attcggacgt gcgccgctga cttcgcgcag acggtcgcag attgcgtcct tgacgcgcag
     5521 cgttgtgaat tcgaggctgc gcagcggaga cttgatcgcg gtgacgtcta cccgcagcgt
     5581 ttcgttcgcc gagaaccact gctcccaacg ctgttcgacc gcgagcgcgt agacgtcctg
     5641 ttcgctgcga tacgggcgat gcgcaatttt cagcaggacg cggctcgcga ggcgcgagtg
     5701 caggttggcg gccatgccgg cggcccagcc gccgcggaaa tgcacgccgc cgggcacttg
     5761 cgcgccggct gtgaacggtg cgccgttcag gtgcttcgcg gcaatttcgg cgagctcggt
     5821 ggcgagcgag gcttcaaggc cgcgcggaca ggggaggaag aaatcgaagg acattgaggt
     5881 tgccggaagg cgttggataa gggcgtattg tacgcggtcg gcgtgggggc tttggcgttt
     5941 gcctacggcg ttggggtttg gttggtctgg ttgtctgcgc ggcgcttgtt tctgtatgcc
     6001 tgtggcgttg gcctttcctt gatctggctc tttggccttt ccttgaattg atattggtct
     6061 attagcgtcg cccctgtgcg gggcgggcac ctacttttct ttgcctgccg cctgccgcaa
     6121 agaaaagtag gcaaaagaaa gcggctcacc ccgccagctc ataaac
//
LOCUS       DATASH010000677         3142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3868577,
            whole genome shotgun sequence.
ACCESSION   DATASH010000677 DATASH010000000
VERSION     DATASH010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3868577"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..1892
                     /gene="oxc"
                     /locus_tag="VHV99_18130"
     CDS             150..1892
                     /gene="oxc"
                     /locus_tag="VHV99_18130"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HEX3637364.1"
                     /translation="MAEADQPTTANTLKPQATETTDGFHLVIDALKLNDIHTIFGLVG
                     IPVTDLARLAQAEGMRFIGFRHEQNAGNAAAISGYITQKPGICLTVSAPGFLNGLTAL
                     ANATTNCFPMILISGSSEREIVDLQQGDYEEMDQLNAAKPYAKAAYRVLHAEDIGIGV
                     ARAIRAAVSGRPGGVYLDLPAKLLAQTLDTVKAQQSLVKVVDAAPRQLPAPESIKRAI
                     DVLKSAKRPLILLGKGAAYAQADAEIRAFVEQSGIPYLPMSMAKGLLPDTHEQSASAA
                     RSFVLQEADVVVLIGARLNWLLSHGKGNTCGAAPKKFVQVDISPTEIDSNVAIAAPVI
                     GDIGSCVAALRAGLDASLAKPSAEWTGAIAERKNRNLAKMAATLDKNPSPMNFHSALR
                     AIRDVLKTRPDINVVNEGANTLDYARSIIDMYEPRKRFDSGTWGIMGIGMGFAIGAAV
                     TTGKPVVAIEGDSAFGFSGMELETICRYDLPVCTVVFNNNGVYRGTDVNPTGGKDVAP
                     TVFVKGARYDRMIEAFGGIGYNATTPEELTKALLEAIASGKPTLINAVIDEAAGTESG
                     RLTNLNPQSAAMKK"
     gene            1980..2791
                     /locus_tag="VHV99_18135"
     CDS             join(1980..2328,2328..2791)
                     /locus_tag="VHV99_18135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587663.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEX3637365.1"
                     /translation="MAKPILDDELWALIEPLLPPPKPRRTRYPGRKPLDNRAVLTGIL
                     FVLQTGLRWDLLPREMGCGSGMSCWRRLRDWQAAGVWVQLHEVLLARLRAADQIDWSR
                     VIVDSSSIRAVGSGPKTGPNPTDRARPGSKHHLITEAQGIPLAVILTGANRNDVTQLQ
                     ALVEAIPPIGGKRGRPLSKPRVVQGDRGYDHDKYRRPLHAAGIATDIARRGEPHGSGL
                     GKTRWVVERTISWLHNFRRLRIRFEQLAFIHEAFLRIACCIICWRHLQKSFC"
BASE COUNT          683 a          949 c          942 g          568 t
ORIGIN      
        1 gctggatcga acactcaggc gcgccagccg gcgtggccgt cgccgaggaa cggctgcaac
       61 cgtcgtccgc ggctcgtctg aatgaatgat cccgtgagca cgccgctcac gatccaactc
      121 atcacgcccc gctacccagg agagatgtca tggcagaagc cgaccagccc acaacagcga
      181 acacgttgaa accacaggcg accgaaacga ccgacggatt ccatctcgtc atcgacgcgc
      241 tgaaacttaa cgacatccac accatcttcg gactcgtcgg catccccgtt accgacctgg
      301 cgcggcttgc acaagcggaa gggatgcgtt tcatcggctt ccgtcatgaa cagaacgcag
      361 gcaacgcggc ggcgatttca ggctacataa cgcagaagcc gggcatctgc ctcaccgtgt
      421 cggcgccggg ctttctcaac ggcttgaccg ccctcgccaa cgcgaccacg aactgttttc
      481 cgatgatcct gatcagcggt tcgagcgaac gcgaaatcgt cgatctgcag cagggggatt
      541 acgaagaaat ggatcagttg aatgcggcca agccttacgc aaaggccgcg tatcgcgtgc
      601 tgcatgcgga ggacatcggc attggcgtgg cgcgcgcgat ccgtgccgcg gtgtctggcc
      661 ggcccggtgg cgtatatctg gacttaccgg ccaagctgct cgctcaaacg ctagacaccg
      721 tcaaggcgca gcagtcgcta gtgaaagtgg tcgatgccgc gccgcgtcag ttgccggcgc
      781 cggaatcgat caagcgcgcg atcgatgtgc tgaaaagcgc aaaacgtccg ctgatcctgc
      841 tcggcaaggg ggcggcgtat gcccaggctg acgcggagat ccgcgctttc gtcgagcaaa
      901 gcggcattcc atatctgccg atgtcgatgg ccaagggctt gttgcccgac acgcacgagc
      961 agtccgcatc ggcggcgcgc tcgttcgtgc tgcaggaggc cgacgtcgtg gtgctgatcg
     1021 gagctcgtct gaactggcta ctgtcgcacg gcaagggcaa cacgtgtggc gcagcgccga
     1081 agaagttcgt gcaggttgat atctcgccga ccgaaatcga cagcaatgtc gcgattgccg
     1141 cgccggtgat cggcgatatc ggctcgtgcg tggcggcact tcgcgcagga ctcgatgcga
     1201 gtttggcaaa accgagtgcc gagtggaccg gtgcgatcgc cgagcgcaag aacaggaatc
     1261 tggcgaagat ggccgcgacg ctcgacaaga acccgtcgcc gatgaatttc cacagcgctt
     1321 tgcgtgcgat ccgtgacgtg ttgaagacgc gtccggacat caatgtcgtc aacgaaggtg
     1381 caaacacgct cgactacgcg cgcagcatta tcgacatgta cgagccgcgc aaacgcttcg
     1441 attcgggcac gtggggaatc atgggtatcg gtatgggttt cgccatcggc gcggcggtaa
     1501 cgacaggcaa accggtggtc gcgatcgaag gcgacagcgc cttcggattc agcggtatgg
     1561 aactcgaaac gatctgccgt tacgacttgc cggtttgcac ggtcgtattc aacaacaacg
     1621 gcgtatatcg cggcaccgac gtgaacccga cgggcggcaa ggacgtcgca cctactgtgt
     1681 tcgtgaaggg tgcgcgctac gacaggatga tcgaggcatt cggcggcatc ggctacaacg
     1741 cgaccacacc ggaagaactg acgaaggcgc tgctcgaggc aatcgcatcc ggcaaaccga
     1801 cgttgatcaa cgccgtgatc gatgaagcgg ccggcaccga gagcggccgc ctgaccaatt
     1861 tgaatccgca aagcgcggca atgaaaaagt aataagagcc cataacaaaa tgaacgaagg
     1921 ggctgttaac cctcgatgaa tgctgttaac cttttcattg tttcgacgac gaaaaggaca
     1981 tggccaagcc gatactcgac gacgaactgt gggcactcat cgaaccgttg ctgccccctc
     2041 ccaaaccacg acgaacccgt tatccggggc gcaagccgct cgacaatcgt gcggtgctca
     2101 ccggcattct cttcgtctta cagacaggtc tgcgttggga cctgctacct cgcgagatgg
     2161 gttgcggcag cggcatgagt tgctggcgtc gcctgcgtga ctggcaagcc gctggcgtct
     2221 gggtgcagtt gcacgaggtg ctgcttgcgc gactgcgcgc agccgaccaa atcgactggt
     2281 ctcgcgtcat cgtcgattcc tcttcaatcc gtgctgtggg ctcaggtcaa aaacaggacc
     2341 gaatcccacg gaccgcgcgc gacccggttc aaaacaccat ctcatcaccg aagcgcaggg
     2401 catcccgctc gcggtgattc tgaccggcgc caaccgcaac gacgtcactc aacttcaagc
     2461 gttggtcgag gcaattccgc caatcggcgg caagcgtggc agaccactat cgaagcctcg
     2521 ggtcgttcag ggcgacagag gctacgacca cgacaagtat cgacgtccac tgcatgccgc
     2581 cggcattgcc accgacatcg ctcgacgtgg tgagcctcat ggtagtgggc taggcaagac
     2641 acgctgggtt gtggagcgga ccatctcgtg gcttcacaac ttccgtcgac tgcgcatccg
     2701 cttcgaacag ctcgctttca tccacgaggc tttcctcaga atcgcctgct gcatcatatg
     2761 ttggcgacac ctgcaaaagt cattctgtta acacctctaa gtggtacagc aatgatgtac
     2821 gtggaaaacg tactacgtta cagcccagac aacgcatgtt gcgttgcgag gttagtctgg
     2881 cgaagcgcac acagcgcgcc gatgacgagt tccgtggttg catagatgca acagtcggcg
     2941 agggcaatag ccggggatga aagttgcgct cggcggacga tgcggacatg acgctgtcta
     3001 accgcaatag cacggccgac aaccacctca tttggcgcga tcctggcgaa cccatcgacg
     3061 gcctcgacgc agtcgtcagg cgcgtggctg gcatccctcg tctgcgtcgg ctcgcgtcgc
     3121 cacttctagc aaagctcata at
//
LOCUS       DATASH010000678         8811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6835375,
            whole genome shotgun sequence.
ACCESSION   DATASH010000678 DATASH010000000
VERSION     DATASH010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 8811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8811
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6835375"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..1719
                     /gene="ettA"
                     /locus_tag="VHV99_18140"
                     /pseudo
     CDS             55..1719
                     /gene="ettA"
                     /locus_tag="VHV99_18140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186903.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
     gene            1942..3063
                     /locus_tag="VHV99_18145"
     CDS             1942..3063
                     /locus_tag="VHV99_18145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006052765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HEX3637366.1"
                     /translation="MAMSKGKRWAIVVGGVLLVLIVVAVGALQFAQREVKERVIAALG
                     PLGSAESIRVGLTSVHLTNVLLKAPPDWPAGDPLRADEIIVTPDIRDLIARRMHIRSV
                     VVRGFDIAVLRNKDGAIRLMPNLRESVNKSDEEAGGVAISAPREKLIDHISFEQGNFH
                     FYDMTVGPPAFKVTVSNANATVDHLQLPALSEPTTVNLTGSIKGPAHTGTVSFGGWIE
                     IANKDSQTTSQLHGVDVVMLDPYLVKKTGAKAQVTGGTVDLTVDSTVRNYQLHAPGTV
                     TIHNLQLADSGNPLDTFMSIPTKAAIAALKSHNGDITLHFTLDGNLRDPKFSVQESLM
                     TRLGTGFAKAMGVSVEGVAKGAGETVKGLGNALKNLLGQ"
     gene            complement(3194..3817)
                     /locus_tag="VHV99_18150"
     CDS             complement(3194..3817)
                     /locus_tag="VHV99_18150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX3637367.1"
                     /translation="MAIKAVVFDFGGVLIDWSPEYLYRQLIPGETERRWFLTHVCSMD
                     WVIRQDGGQPIVEATDELIAKFPDHAPLIRAFYERWHEMVAGVLEEGVAIMEKLEAAN
                     VPLFGLTNWSAETFPYAWEHYPVLRRFRDTVVSGRVRLVKPDPAIFAEMRQRIEAQLP
                     GIEPDQLVFIDDNLKNAEAATALGWHGVHHTSAARTEARLRELGLPV"
     gene            complement(3850..5664)
                     /locus_tag="VHV99_18155"
     CDS             complement(3850..5664)
                     /locus_tag="VHV99_18155"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEX3637368.1"
                     /translation="MNARLPETSSIPERLATLRNAMAREGVAAYLVPSADPHLSEYLP
                     ERWQGRQWLSGFTGSAGTLVVTADFAGVWTDSRYWEQASAQLAGTGVQLMKMTGGQQS
                     APHFEWLAENVASGASVGVDGAVLGVSAARALSQALTARDVKLRTDVDLFDAIWPQRP
                     SLPAAAVFEHAAPHASIARSEKLAQIRRAMAEKGAQWHFISTLDDLAWLLNLRGADVS
                     YNPVFVAHALIGLESASLFVADGKVPQALADALARDNIRVEPYAKAAEALAALPVGST
                     LLIDPRHITYGSLQSVPSTVKVVEAVNPSTFFKSRKTEADAAHVRETMEQDGAALAEF
                     FAWFEGALGRETITELTIDERLTAARARRPGFVSLSFATIAGFNANGAMPHYRATAEA
                     HSVIEGNGLLLIDSGAQYLSGTTDITRVVPIGTISEAQRRDFTIVLKGTMALSRAKFP
                     RGIRSPMLDAIARAPIWEAGADYGHGTGHGVGYFLNVHEGPQVISHYAPAEPWTAMEE
                     GMITSVEPGLYRPGKWGVRIENLVLNVAAEKTEFGDFLKFETLTLCPIDTRCLDLSLL
                     RDDERAWLNAYHETVRTRLSPHVSGEAKAWLELRTQPI"
     gene            complement(5684..5947)
                     /locus_tag="VHV99_18160"
     CDS             complement(5684..5947)
                     /locus_tag="VHV99_18160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637369.1"
                     /translation="MGWPGILVLGAVVGLAGGWLHPLRRSSGSNRVQWWIALLAGLLG
                     AGVARMAGNVVGLFHDGDTLEWPVCTAVALVAVAVTVGLLSRR"
     gene            6166..6876
                     /locus_tag="VHV99_18165"
     CDS             6166..6876
                     /locus_tag="VHV99_18165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3637370.1"
                     /translation="MRIALIEPDLRHATLVGQLIFAGGHVCQHFIASTPFLHRAAEEF
                     FDLLITESWAGDHSAEDVIPRARSILPSLPVIVLFSEPRESQIVAALHAGADDCLTKP
                     VRGPEMLARVDALLRRTGLRRPPNRRRDLIGGYAFDAAHFAVTFRNHTVALTPKEYRF
                     ALLLFTNLSRPVSRVHILETVWTRRRDVRSRTVDTHASRVRTKLQLRPEFGYCLTPLY
                     GYGYQLDAVPPEVVVDAG"
     gene            6939..8225
                     /gene="hemA"
                     /locus_tag="VHV99_18170"
     CDS             6939..8225
                     /gene="hemA"
                     /locus_tag="VHV99_18170"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEX3637371.1"
                     /translation="MQLLTIGINHHTAPVALRERVAFPLEQIKPALDTFKSVWLGRTA
                     RSAPEAAILSTCNRTELYCATDDQAAREAAIQWLSKYHNLPIEELAPHVYALPQSEAV
                     RHAFRVASGLDSMVLGETQIVGQMKDAVRTASEAGALGTYLNQLFQRTFAVAKEVRST
                     TEIGSQSVSMAAAAVRLAQRIFDKVSNQRVLFIGAGEMIELCATHFAAQQPRELVVAN
                     RTAERGTRLAERFNGRAIPLSELPVRLHEFDIIVSCTASTLPIIGLGAVERAVKARRH
                     RPIFMVDLAVPRDIESEVGKLEDVFLYTVDDLGAIVREGNASRQAAVAQAEAIIETRV
                     QNFMQWLDARSIVPVIRHMHTQADVLRRAEVERAQKMLARGDDPAAVLEALSQSLTNK
                     LIHGPTHALNRASSENRDTLIELMSGFYKHSGSSER"
     gene            8344..>8811
                     /locus_tag="VHV99_18175"
     CDS             8344..>8811
                     /locus_tag="VHV99_18175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HEX3637372.1"
                     /translation="MKTSMQRKLDQLTIRLAELNDLLSREDITSNLDQYRKLTREHAE
                     LGPVVEHYALWRHAMNDAATAQELLADVSMRDFAEEEIRAARERMEKLGGELQKMLLP
                     KDPNDDRNIFLEIRAGTGGDESALFAGDLLRMYLRFAERNRWQVEMMSASESDL"
BASE COUNT         1731 a         2873 c         2712 g         1495 t
ORIGIN      
        1 ccctcgactg ccagcccgca cgcggccggc gtcgtcaacg tctactgcaa gcaaatggcc
       61 caatacgttt tcaccatgaa ccgggtcggc aagatcgtgc cgcccaagcg ccagatcctg
      121 aaagacatct cgctatcgtt cttccccggc gcgaagatcg gcctgctcgg cctgaacggc
      181 tcgggcaagt cgacgctgat ccgcatcatg gcgggtgtgg acacggaaat cgaaggcgaa
      241 gcgacgccga tgccgaacct gaacatcggc tatctgccgc aggaaccgca gctcgatccg
      301 caaaagaccg tgcgtgaagc ggtcgaggaa ggcctcggcg acgtgttcaa cgcgcagaag
      361 aagctcgacg aaatctacgc ggcctacgcc gagccggacg ccgacttcga cgcgctcgcc
      421 gccgagcagg cgaagtacga agcgatcctc gcaacaaccg acggcagcgc cgagcagcag
      481 atcgaaatcg ccgccgacgc gctgcgtctg ccagcgtggg acgcgaagat cgagcatctg
      541 tcgggcggcg aaaagcgtcg cgtcgcactg tgcaagctgt tgttgcagaa gcccgacatg
      601 ctgctgctcg actagccgac caaccacctc gacgcggaat cggtcgaatg gcttgagcaa
      661 ttcctcacgc gcttcccggg caccgtggtc gccgtcacgc acgatcgcta ctttctcgat
      721 aacgcagccg agtggattct cgaactcgac cgcggccacg gcattccgtg gaaaggcaac
      781 tacagcagct ggctcgatca gaaggaagag cgtcttaagc aggaagaatc atcggaatcg
      841 gcgcgtcaga aggcgatcaa gaaggagctg gagtgggtgc gccagaatcc gaagggccgt
      901 caggcgaagt cgaaggcgcg tatcgcccgc ttcgaggaac tgaacagcca ggactatcag
      961 aagcgcaacg aaacgcagga aatcttcatt ccggtcggcg atcgtctcgg caatgaagtg
     1021 atcgagttca agaacgtcag caagtcgtat ggcgaccgtt tgctgctcga cgacgtcagc
     1081 ttcaagattc cggcgggcgc gatcgtcggc atcatcgggc cgaacggcgc cggtaagtcg
     1141 acgctgttcc gcatgctgac cggccgtgaa cagccggatt cgggcgagat cgtgcaaggc
     1201 ccgaccgtca agctcgcgta tgtggaccag agccgcgacg cgctcgatgc atcgaaaacc
     1261 gtgttcgaag agatttccgg cggtgcggac gtgctgacgg tcggcaagta cgaaacgccg
     1321 tcgcgtgcgt acatcggccg cttcaacttc aagggtggcg atcagcagaa aatcgtcggc
     1381 aacctgtcgg gtggtgagcg cggccgcttg catctggcga agacgctgat cgcgggcggc
     1441 aacgtgctgc tgctggacga accgtcgaac gatctcgacg tcgaaacgct gcgtgcgctt
     1501 gaagacgcct tgctcgaatt cgcgggctcg gtgctggtca tctcgcacga tcgctggttc
     1561 ctcgaccgca tcgcgacaca catcctcgcg ttcgaaggcg attcgcaaat caccttcttc
     1621 gacggtaatt accaggaata cgaagcggac aagcgcgccc gtctcggtga agaagctgca
     1681 cgtccgaagc gcctgcgtta caagccgatc gcgcgataaa tcgcgaacct ggcggcggcc
     1741 ggtgcaccgt ggcgggcgcc gccaacgcga ggcgttcggc ggatgagcgc cagggcacac
     1801 gttttgcgtg ccatggcgcg atacacgttg tcccgccagg atgcagcaac tccgccgagg
     1861 caaatcaatg cgtgtcccac tggcgcgcgc acaggccgat cacacccaca ccgcgcacgg
     1921 cgcggcgacg aggaggcaag catggcgatg tcgaagggca agcgctgggc cattgtcgta
     1981 ggcggtgtgt tgctggtgct gattgtcgtc gcggtcggtg cgctccagtt cgcgcagcgc
     2041 gaagtcaagg aacgcgtgat agcggccctc ggcccactcg gcagcgccga aagcatccgg
     2101 gtcggcctca cctcagtcca tctcaccaac gttctgctca aggcgccgcc cgactggccc
     2161 gccggcgacc ctttgcgggc cgacgaaatc atcgtcacgc ctgacattcg cgatctgatc
     2221 gcgcgccgca tgcatattcg cagcgtggtg gtgcgcggct tcgacatagc ggtgttgcgt
     2281 aacaaggacg gcgcgatccg cctgatgccg aatttgcggg aatccgtgaa caagtccgat
     2341 gaagaagccg gcggcgtcgc gatatccgcc ccccgcgaaa agctgatcga ccacatcagc
     2401 ttcgaacaag gcaacttcca tttctacgat atgacggtcg gaccgccggc gttcaaggtg
     2461 acggtcagca acgcgaacgc gacggtcgat cacctgcaac tgcccgcgct cagcgaaccg
     2521 accacagtca acctcaccgg ctcgatcaaa ggcccggcgc atacgggcac ggtttcgttc
     2581 ggcgggtgga tcgagatcgc caacaaggat tcgcagacca ccagccaact gcacggcgtc
     2641 gatgtggtga tgctcgatcc ttacctggtg aaaaaaaccg gcgcgaaggc gcaagtgaca
     2701 ggcggcacgg tcgacctgac agtcgactcg accgtgcgta actatcagct gcacgcgccc
     2761 ggcacggtga cgatccacaa tctgcaactc gccgacagcg gcaatccgct cgacaccttc
     2821 atgtcgatcc cgaccaaggc cgccatcgcc gcgctcaagt cgcataacgg cgatatcacg
     2881 ctgcatttca cgctggatgg aaatttgcgc gacccgaagt tttcggtgca ggaaagtctg
     2941 atgacgcgcc tcggcaccgg ttttgcgaaa gcgatgggcg tgagcgtcga aggcgtggcg
     3001 aagggcgccg gggaaaccgt gaaaggcctg ggtaacgcgt tgaagaatct gctcgggcag
     3061 taggcagggc gaggcggacg gcgccgctca gcgttggacg ttagccttta cgcgtcgggc
     3121 gtcgggcgtt gggcgttggg cgagcttcaa agcgaagccg catcgcagcc tcgcttcaac
     3181 cggcatcccg cgttcaaacc ggcaagccca actcgcgtag cctcgcttca gttcgcgccg
     3241 cgcttgtgtg atgtaccccg tgccagccca atgccgttgc ggcttcggcg tttttcagat
     3301 tgtcgtcgat gaagacgagt tggtccggct caatgcccgg caattgcgcc tcgatccgct
     3361 gacgcatttc cgcgaaaatc gccggatccg gtttcaccag cctcaccctg ccggacacga
     3421 cggtgtcgcg gaatcgccgc aacacggggt agtgttccca tgcataggga aacgtctcag
     3481 ccgaccagtt cgtcagcccg aaaagcggca cattggccgc ctcgagtttt tccatgatcg
     3541 cgacgccctc ttccagcacg ccggccacca tctcgtgcca gcgttcgtaa aacgcgcgga
     3601 tcagcggagc gtgatcggga aacttcgcga tcagttcgtc tgtggcctcg acgatcggct
     3661 ggccgccgtc ctggcggatc acccagtcca tcgaacagac atgagtcaga aaccagcggc
     3721 gctccgtttc gccaggaatc aactgacggt acagatactc gggactccag tcgatcagca
     3781 cgccgccgaa atcgaacacc actgccttga tggccatgca cgctccgtca gtcaactcgt
     3841 gcgacgagat cagataggtt gggtgcgcag ctcgagccac gccttggcct cgcccgagac
     3901 atgcggcgac aggcgcgtgc gcaccgtctc gtgataggca ttgagccatg cgcgctcgtc
     3961 gtcacgcaac agcgacaggt cgaggcagcg cgtgtcgatc gggcacaggg tcagcgtctc
     4021 gaacttgagg aaatcaccaa actcggtttt ttccgcggcc acgttcagca cgaggttttc
     4081 gatccgcacg ccccacttgc ctggccggta aaggcccggc tcgaccgagg tgatcatgcc
     4141 ttcttccata gccgtccacg gttcggccgg cgcgtagtgc gaaatcacct gcgggccttc
     4201 gtgcacgttc aggaaatagc ccacgccgtg accggtgccg tgaccataat cggcgccggc
     4261 ttcccagatc ggcgcgcggg caatcgcgtc cagcatcggc gaacggatgc cgcgcgggaa
     4321 tttggcgcgc gacagcgcca tggtgccttt cagcacgatc gtgaaatcgc gccgctgtgc
     4381 ctcgctgatg gtgccgatcg gcacgacgcg ggtgatgtcg gtcgtgccgc tcagatactg
     4441 tgcgcccgaa tcgatcagca gcaggccatt gccctcgatc accgaatgcg cttcagcggt
     4501 cgcgcggtag tgcggcatcg cgccgttcgc gttgaagccg gcgatcgtcg caaagctcag
     4561 cgacacgaag cccggacggc gcgcgcgggc tgccgtcagg cgctcgtcga ttgtcagctc
     4621 ggtgatcgtt tcgcggccca acgcgccttc gaaccacgcg aagaattcgg cgagtgccgc
     4681 gccgtcctgt tccatcgtct cgcgcacatg cgccgcgtca gcctcggtct tacgcgattt
     4741 gaagaacgtg gacgggttga ccgcctcgac gaccttcacc gtggacggca ccgattgcag
     4801 cgagccatac gtaatgtggc gcgggtcgat cagcagtgtg ctgccgacgg gcagcgcggc
     4861 cagcgcctcg gctgccttcg catacggttc gacacggatg ttgtcgcgcg cgagcgcatc
     4921 ggccaacgct tgcggcacct tgccgtcggc aacgaagagc gacgcgcttt ccagaccgat
     4981 cagcgcgtgc gccacgaaca ccgggttgta gctgacgtcg gcgccgcgca gattcaaaag
     5041 ccatgcaagg tcgtcgagcg tggaaatgaa atgccactgc gcgcccttct ccgccattgc
     5101 gcggcgaatc tgcgcgagct tttcggaccg ggcgatgctc gcgtgcggcg cggcatgctc
     5161 gaacacggcg gcagcgggca gcgacggccg ctgcggccag atcgcgtcga acagatcgac
     5221 gtcagtgcgc agcttcacgt cacgcgcggt gagcgcctgg ctcaacgcac gcgccgccga
     5281 cacaccgagc acggcgccgt ccacaccaac cgaagcgccg ctcgcgacgt tctccgcgag
     5341 ccattcgaaa tgcggcgcgc tctgctgccc gcccgtcatt ttcatcaact gaacgccggt
     5401 gccggccagt tgcgcgctgg cttgttccca atagcggctg tcggtccaca cgccggcgaa
     5461 atcagcggtc acaaccagcg tgccggctga gccggtgaaa cccgacaacc actgccggcc
     5521 ttgccagcgc tcggggagat attcggaaag atgtggatcc gcggacggca ccagataggc
     5581 ggctacgcct tcgcgcgcca tcgcattgcg caatgtcgcg agccgttcgg ggatggagga
     5641 ggtttcgggg agtcgggcgt tcatcggagt cacctgcaaa tattcatcga cgggaaagca
     5701 agccgaccgt cacggcaacg gcgaccagcg caacggcggt acataccggc cattcgagcg
     5761 tatcgccgtc atgaaagaga ccgaccacgt tgccagccat gcgcgcgaca cccgcgccga
     5821 gcaggccggc cagcagcgct atccaccatt ggacgcgatt cgagccgctc gagcggcgca
     5881 acggatgcag ccacccgccc gcgaggccga ccaccgcgcc cagcaccagg attccgggcc
     5941 accccattag cagcagctca cacggcagca actcgcgcgc aatttcagat tcgtctcatg
     6001 ggcgcaaggg gccaggacag ctagcatttt tgtctcatta actataaacg ttcagcacgg
     6061 cgctttccgc atagcggttg agtttagggg ccggggtgat gttaggcaag ctgtaagcct
     6121 tacagagaag cctggtcctt gttaatgcca agctgaaagc gccccatgcg aatcgccctc
     6181 atcgagccgg accttcgaca cgccaccttg gtcggccagc tgatttttgc cggcggccat
     6241 gtgtgccagc acttcatcgc aagcacgccg ttcctccatc gcgctgccga agaattcttc
     6301 gatcttctca tcaccgaaag ctgggccggc gaccattccg ccgaagacgt gattccgcgt
     6361 gcccgctcca tcctgccgag cttgccggtg atcgtgctgt tctcggaacc gcgcgagagc
     6421 cagatcgttg ccgcgctgca cgctggagcc gacgactgcc tgaccaaacc ggtccgcggg
     6481 ccggaaatgc tggcccgcgt ggacgcgtta ctgcggcgca cgggcttgcg gcggcccccc
     6541 aaccggcgtc gcgacctgat cggcggctat gcgttcgacg cagcgcactt cgccgtcaca
     6601 ttccgcaatc acactgtcgc cctcacgccg aaggaatacc gctttgcgct gctgctgttt
     6661 accaacctgt cgcggccggt atcgcgcgtg catattctcg aaaccgtctg gacacgccgc
     6721 cgcgacgtaa ggtcacgtac tgtcgacacc catgcgtcgc gcgtgcggac caaattgcag
     6781 ttgcgcccgg aattcggcta ttgcctgaca ccgctttacg gctacggcta tcagctcgac
     6841 gcggtcccac cggaggtcgt tgtagacgcg ggatgacgcg tgcgccaggt aagaaacgtg
     6901 aaaacgctat aatattgggc taaaccatag tcccccatat gcagcttcta acgatcggaa
     6961 tcaatcacca cactgcgcct gtcgccttgc gcgaacgcgt ggcgtttccg ctcgaacaga
     7021 tcaagcccgc attggacacc ttcaagagcg tctggctggg ccgcaccgcc cgctcggcgc
     7081 cggaagccgc cattctgtcc acctgcaacc gcaccgagct ctactgcgcg accgacgacc
     7141 aggccgcgcg tgaagccgcg atccagtggc tctcgaagta ccacaacctc cccatcgagg
     7201 aactcgcgcc ccatgtttac gcgctgccgc aatcggaagc cgtgcgccac gcgttccgcg
     7261 tcgcctcggg gctcgattcg atggtgctgg gcgagacgca aatcgtcgga caaatgaagg
     7321 atgcggtgcg cacggcgtct gaagccggtg cgctcggcac ctatctgaac cagttgttcc
     7381 agcgcacctt cgccgtcgcg aaggaagtgc gcagcacgac cgaaatcggc tcgcagtcgg
     7441 tttccatggc ggcagccgct gtgcggctcg cgcagcggat tttcgacaag gtgtcgaacc
     7501 agcgcgtgct gttcatcggc gcgggcgaaa tgatcgagtt gtgcgccacg cacttcgcgg
     7561 cgcagcagcc gcgcgaactc gtcgtcgcga accgcaccgc cgagcgcggc acgcgcctcg
     7621 ccgaacgctt caacggccgg gccattccgc tttcggaact gcccgtgcgc ctgcatgagt
     7681 tcgacatcat cgtgtcgtgc accgcctcca ctctgccgat catcggcttg ggcgcggtcg
     7741 agcgggccgt gaaggcgcgt cgtcaccggc cgattttcat ggtcgatctg gcggtgccgc
     7801 gcgatatcga atccgaagtc ggcaagctcg aagacgtctt cctgtacacc gtcgacgatc
     7861 tcggcgcgat cgtgcgcgaa ggcaatgcgt cgcggcaagc cgcggtcgcg caagccgaag
     7921 cgatcatcga aacgcgcgtg cagaacttca tgcaatggct cgacgcgcgc agcatcgtgc
     7981 cggtgatccg ccatatgcat acgcaggccg acgtgctgcg tcgcgccgaa gtcgaacgcg
     8041 cgcagaaaat gctcgcgcgc ggcgacgacc cagccgcggt gctcgaagca ctgtcgcaat
     8101 cgctcaccaa caaactgatc cacggcccca cgcacgcgct caatcgcgcg agcagcgaga
     8161 accgtgacac gctcatcgag ttgatgagcg gcttctacaa acactccggt tcctcggagc
     8221 gttagcggcg cagccactgc cgcaccgtcc gcgccaccct gtggcccgcg acgtgcttct
     8281 gaccagggca tccgcccact cccctatgtc gtccttgccg gaatccgctc cgatacccgt
     8341 ccgatgaaaa cgagcatgca acgcaagctc gaccagctca ctatccggct ggccgaactg
     8401 aacgacctgt tgagccgcga ggacatcacc tcgaatctcg accaataccg caagctcacg
     8461 cgtgagcacg ccgagctcgg gccggtcgtc gagcattacg cgctgtggcg ccacgcaatg
     8521 aacgacgcgg cgaccgcgca ggaactgctg gcggacgtgt cgatgcgcga cttcgcggaa
     8581 gaagaaattc gcgcggcacg cgagcggatg gaaaagcttg gcggcgaatt gcagaaaatg
     8641 ctgctgccga aagacccgaa cgacgaccgt aacatcttcc tcgaaatccg cgccggaaca
     8701 ggcggcgacg aatcggcgct gttcgcgggc gatttgctgc gcatgtatct gcgctttgcc
     8761 gaacgcaatc gctggcaggt cgagatgatg tcggcgagcg aatcggactt g
//
LOCUS       DATASH010000679         2618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3671034,
            whole genome shotgun sequence.
ACCESSION   DATASH010000679 DATASH010000000
VERSION     DATASH010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3671034"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(231..1031)
                     /locus_tag="VHV99_18180"
     CDS             complement(231..1031)
                     /locus_tag="VHV99_18180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011544895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX3637373.1"
                     /translation="MQRRFILKLAATLGAASLFAAATAAHAEDSIKVGVTGGPHAQIM
                     EVVKTVAAKNGLNIKIVEFSDYVQPNAALAGGDLDANSYQHDPYLQAQVKDRGYKLIR
                     VADTVTYPMGIYSKKVKTLAELQPGAKIAVPNDPTNGGRALLLLQKQGLLKLRADAGL
                     KATPLDIVDNPKKLKIVELDAAQIPRSLNDVDAAAINTNFAMEAGLKPRQDAIAIEDP
                     KGPYVNIIAIREADRNKPWVAKLVAAYHSPEVKQFVESKFGGSVITAW"
     gene            complement(1079..1732)
                     /locus_tag="VHV99_18185"
     CDS             complement(1079..1732)
                     /locus_tag="VHV99_18185"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HEX3637374.1"
                     /translation="MLSEMFDMFVQSFWETVVMVGISGLVGAAVGLPLGVLLYLTDRQ
                     GVLQNIAVNRVMGVVVNAVRSTPFIILLVAVIPFTRLVVGSSIGTAAAVVPLTIAAAP
                     FIARLVETALREVDRGLIEAAQAMGATTSQIVFKVLLPESLPGVVAGLTITFVSLVGY
                     SAMAGAIGGGGLGDLGIRYGYQRFLPEVMVTVVVILIVFVQLVQSFGDWLVRRLSHK"
     gene            complement(1722..>2618)
                     /locus_tag="VHV99_18190"
     CDS             complement(1722..>2618)
                     /locus_tag="VHV99_18190"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEX3637375.1"
                     /translation="LVRTLNLLTRPSEGNVVVNGRDLTTLPAAQLREARREIGMIFQH
                     FNLLSSRTVYENVALPLELAGMKRDEIEANVLPLLELVGLSAQKDRYPAQISGGQKQR
                     VGIARALASKPKVLLSDEATSALDPETTRAILDLLKRINRELNLTIVLITHQMDVIKQ
                     VCDRVAVLDAGRVVEEGKVIDVFLQPHHEVTRALIGDVIAQELPPAMKARVAERLKTG
                     SGHLLRLAFTGSGVDQPILSETIRRYELDFNILHGQIDEIQGQAFGSLAVLAGGEPAN
                     VAQALTYLREQGVVVEELSYVE"
BASE COUNT          518 a          854 c          747 g          499 t
ORIGIN      
        1 atacgtcaac ccaacgaaca ttataacgac tgcactttcg cggcagccgt gtcgcgggtc
       61 ttctaacagg aggatatcgc tcctcagcgg cgtgacggca atatcgaacg gtcgttctat
      121 tttaatctcg aaaaaacccg ggagacaacc ccgggttaag acctacgact ctaattttgc
      181 attgccgtcg tgtcttgcta tgtgaaacgg tcgaaaaaac ggccgtcaaa tcaccaggcg
      241 gtgatcaccg acccgccgaa cttgctctcg acaaactgct tcacttccgg cgaatgatac
      301 gccgcgacca gcttcgccac ccacggcttg ttcctgtcgg cttcgcggat ggcgatgatg
      361 ttcacgtacg gacccttcgg gtcttcgatc gcgatggcgt cctgcctggg cttcagacct
      421 gcttccatcg cgaagttggt gttgatcgca gccgcgtcca cgtcgtttaa cgaacgcgga
      481 atttgcgcgg cgtcgagttc gacgatcttg agcttcttcg ggttatcgac gatgtcgagc
      541 ggcgttgcct tcagacccgc atccgcgcgc agcttcagca gaccctgctt ttgcagcaac
      601 aggagtgccc ggccgccatt ggtcggatca ttcggcaccg cgatcttcgc gcccggctgc
      661 aattcagcga gcgtcttcac tttcttcgag tagatgccca tcgggtacgt gaccgtatcg
      721 gcgacacgga tcagcttgta gccgcggtcc ttcacctggg cctgcaagta cgggtcatgc
      781 tggtagctgt tcgcgtccag gtcgccgccg gcgagcgctg cgttcggctg cacgtaatcc
      841 gaaaactcca caatcttgat gttcagaccg ttcttcgcgg cgacggtctt cacgacctcc
      901 atgatctgcg cgtgcgggcc gccggtgacg ccgaccttga tcgaatcttc agcgtgagcc
      961 gccgtggcgg cagcgaagag cgacgcggcg ccgagcgtgg cggccagctt gagaatgaaa
     1021 cgacgttgca tgatggacct tttttcgatt ctgtttggcg ggttccaatg acccttgttt
     1081 atttatggct caaacgacgc acgagccaat ccccgaacga ctgcaccagt tgcacgaaga
     1141 cgatcagaat caccacgacc gtcaccatca cttccggcaa aaaacgctga tacccgtaac
     1201 gaatgcccag gtcgccgagc ccgccgccgc cgatcgcacc ggccatcgcc gagtagccga
     1261 ccagcgacac gaaggtaatc gtcaagccgg cgaccacgcc cggcagcgat tccggcaaca
     1321 gcaccttgaa gacgatctgg ctggtagtcg cgcccatcgc ctgcgcggct tcgatcagac
     1381 cgcgatcgac ttcgcgcaac gctgtctcaa ccagacgcgc gatgaacggc gccgcggcga
     1441 tcgtcaacgg caccacggcc gcagccgtgc cgatcgacga acccaccacg agacgggtga
     1501 acggaatcac cgcaaccagc aagataatga acggcgttga gcgcaccgca ttgacgacca
     1561 cgcccatcac acgattcacg gcgatgttct gcagcacgcc ctgacggtcc gtcagatata
     1621 gcagcacgcc gagcggcaga ccgaccgccg cgccgaccag tccggaaatg cccaccatca
     1681 cgactgtttc ccagaacgac tggacgaaca tatcgaacat ttcactcaac atacgacagc
     1741 tcctccacca ccacaccctg ttcgcgcaga tacgtcagcg cctgcgccac gttcgccggt
     1801 tcgccgcccg cgagcaccgc gagcgagccg aacgcctgcc cctggatctc gtcgatctgg
     1861 ccatgcagga tgttgaagtc aagctcgtag cggcgaatcg tttccgacag aatcggctga
     1921 tcgacgcctg agcccgtgaa cgcgagccgc agcaagtggc cgctgccggt cttcagacgt
     1981 tccgcgacgc gcgccttcat cgcgggcggc aattcctgcg cgatcacgtc gccgatcagc
     2041 gcgcgcgtga cctcgtgatg cggttgcagg aacacatcga tcaccttgcc ttcttcgacc
     2101 acccggcccg catccagcac cgcaacgcgg tcgcagacct gcttgatcac atccatctgg
     2161 tgcgtgatca gcacgatcgt caggttcagt tcgcgattga tgcgcttgag cagatcgaga
     2221 atcgcacgcg tggtttcggg atcgagcgcg gaggttgctt cgtcggaaag cagcaccttg
     2281 ggcttgctcg ccagcgcgcg cgcgatgccg acgcgctgct tttgcccacc gctgatctgc
     2341 gccggatagc gatctttctg cgccgacaag ccgaccagtt cgagcaacgg cagcacgttc
     2401 gcttcgattt cgtcgcgctt catgccggcg agttcgagcg gcaacgcgac gttctcgtac
     2461 acggtgcgcg acgacagcag gttgaagtgc tggaagatca tgccgatctc gcggcgcgcc
     2521 tcgcgcaatt gcgcggcggg cagcgtggtc agatcgcggc cgttgacgac aacattgcct
     2581 tcggacggac gcgtcagcag gttgagggtg cgcacgag
//
LOCUS       DATASH010000680         2522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6241157,
            whole genome shotgun sequence.
ACCESSION   DATASH010000680 DATASH010000000
VERSION     DATASH010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6241157"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VHV99_18195"
     CDS             <1..606
                     /locus_tag="VHV99_18195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HEX3637376.1"
                     /translation="EDAAFAPLSDEERARAQSFRRHEDALRFASVRAALREQLARYLG
                     TAAHAVRFKLDANHRPHLANSDAYDFNVSHAGAHGLIAMSPRRRVGVDIEQYSDKFDW
                     RSIASLTLDASEAAWIEQLGVPQQFAAFYDAWVAKEALVKTTGVGITRGLQHLTVLPR
                     DSAEVRLRNQIPHDMREVAAQWVVAPQAYAACVAWSMTTFR"
     gene            764..949
                     /locus_tag="VHV99_18200"
     CDS             764..949
                     /locus_tag="VHV99_18200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637377.1"
                     /translation="MQLARAWHGDAARSVASQRMRDELRRGHADDSVCVSSRVIGLQA
                     VCVIRCLGVDAVTHTRQ"
     gene            complement(986..1465)
                     /locus_tag="VHV99_18205"
     CDS             complement(986..1465)
                     /locus_tag="VHV99_18205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637378.1"
                     /translation="MDFRSQYSSRPELVDDHVTDTTDAPERTVLSAVRTWLRPACETA
                     RGGVHWRDILSGVGLRQDGIEHFDLLMRSLMHVSYRPLDMRCRCASDLGKDEAVILQT
                     IALLQKTHSGAALRMLNEWLPAPAVSGVLKLIRWFAIDLLDVGVELDVQARRVTYMH"
     gene            1704..>2522
                     /locus_tag="VHV99_18210"
     CDS             1704..>2522
                     /locus_tag="VHV99_18210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HEX3637379.1"
                     /translation="MSTKSFDAASTSRSTSTSAEAAQRLPTLLLAAGLSLGSAIALGL
                     ARFAYALLLPSMKLDLGWSFAQAGAMNTANALGYLLGALAFPRLARRWSAAALFGAGC
                     VATALLMAVSGLLADTHSLLVLRVMTGASSAAIFISGGVLAARLASARPNDAGLVLGL
                     YYGGTGWGIVASSVLVPLTIFNVVHGWQFAWFALAAACAAFAAVAIGAARKIESVHAI
                     GSSSTQATDATDAPRWPRFSPALAGYGLFGVGYIGYMTFIVALLRNAGMSAVVVT"
BASE COUNT          426 a          797 c          808 g          491 t
ORIGIN      
        1 gaggatgcgg ccttcgcgcc actcagcgac gaagaacgcg cgagagccca aagctttcgc
       61 cgccacgaag acgcgcttcg tttcgcgtcc gtacgtgcgg cgctgcgcga gcaactcgca
      121 cggtatctcg gcactgcggc acacgccgtc cggttcaagc tcgatgcgaa ccaccgtcct
      181 catctcgcca attccgacgc atacgatttc aacgtctccc atgcgggcgc gcatggactg
      241 atcgcgatgt cgccgcgccg gcgcgtcgga gtcgatatcg agcagtacag cgataaattc
      301 gattggcgtt cgatcgcatc attgacgctc gatgccagcg aggcggcatg gatcgagcag
      361 ctaggcgtac cgcagcagtt cgccgcgttt tacgacgcat gggtcgccaa ggaagcgctc
      421 gtcaaaacca cgggtgtggg catcacgcgc ggcttgcagc atctgacggt gctgccgcgt
      481 gacagcgccg aggtgaggct gcgcaatcag attccgcatg acatgcgcga ggttgcagcg
      541 cagtgggtcg tggcaccgca ggcttatgcc gcatgtgtgg cgtggtcaat gaccacgttc
      601 cgttaagacg tccagggctg aacgtcacca cgcgcgcact gataaatcgt ttgcgtaacg
      661 ccgggttcgg atgaagccgg gcgcgctcgc gctctagctt cgacagccac gcatcgcctt
      721 cctgcactgc caccggcaac cattcacaac ggaaccgcca caggtgcaac tcgcccgcgc
      781 gtggcacggc gacgccgcgc ggagcgtggc gagtcagcga atgcgagacg aattgcggcg
      841 cggccacgcc gacgatagcg tgtgcgtgtc gagcagggtg atcggcttgc aagccgtgtg
      901 cgtgattcgt tgcctgggag tcgacgccgt cacgcacacg cggcagtgag agataagcga
      961 gttccaacgt catgcgctcg ggttctcaat gcatatacgt gacgcggcgc gcttgcacgt
     1021 caagttcgac cccaacgtcg agcagatcga tggcgaacca gcggatcagc ttcaacacgc
     1081 cgctgacggc cggcgccggc agccattcgt tcaacatgcg caacgccgcg ccgctgtgcg
     1141 ttttttgcag cagcgcgatg gtttgcagaa tcacggcttc gtccttgccg agatcgctgg
     1201 cgcagcggca tcgcatgtcg agcggacgat acgacacgtg catcagcgaa cgcatcagca
     1261 ggtcgaaatg ttcaatgccg tcttgccgca agcccacgcc gctcaggatg tcgcgccagt
     1321 gcacgccgcc gcgcgccgtt tcacacgccg gccgcagcca ggtgcgaacg gcgctgagga
     1381 ccgtgcgctc gggcgcgtcg gtggtatccg tgacgtgatc gtcgaccagt tcgggacggc
     1441 tgctgtactg agagcggaaa tccatggttg ctcctgaatg gacacggtgt tcggcgtgat
     1501 ggcatgggcc tcatcatgcg gcaatttcct cgtaaatgca aatcattctc atctacggat
     1561 gatgtttggc ggagcattgg cctgccggct ggactggctt gagcgcctcg tgatcacggc
     1621 gctgtgcccc tcaatgcgtc cacagatatg atggcgaaat gccgcagtaa gcctgccgca
     1681 tccttgtcta cccacgctag ccaatgtcca caaaatcttt cgatgcagcg tcgacctccc
     1741 ggtccacctc aacctccgcc gaagcggcgc agcgcctccc caccttgctg ctggccgccg
     1801 gcctgtcgtt aggcagcgcg atcgcgctgg gtcttgcgcg cttcgcgtac gcgctgctgt
     1861 tgccgtcgat gaaactcgat ctcggctgga gcttcgctca ggccggcgcc atgaataccg
     1921 ccaatgcgct gggctatttg ctgggcgcgc tggcttttcc gcggctcgcg cgccgctggt
     1981 cggccgctgc cttgttcggt gccggttgcg tggccactgc gcttttgatg gccgtcagcg
     2041 gtctccttgc cgacacccat tcgctactcg tattgcgcgt gatgaccggc gcgagcagtg
     2101 cggcgatttt catcagcggt ggtgtgctgg cggcgcggct tgcgtcggcg agacccaacg
     2161 acgcgggact cgtgctcggc ctgtattacg ggggcaccgg ttggggcatc gtcgcttcgt
     2221 cggtgctggt gccgctgacg attttcaacg tcgtgcacgg ctggcagttt gcgtggttcg
     2281 ccctcgcggc ggcgtgcgcc gcgttcgccg cagtcgcgat cggcgcagcg aggaaaatcg
     2341 aaagcgtgca cgccattggg tcttcgtcca cacaagcaac cgacgcaacc gacgcgccgc
     2401 gttggccacg tttcagtccc gcgctggcag gctatggatt gttcggtgtc ggctatatcg
     2461 gctacatgac cttcatcgtc gcgctgctgc gcaacgcggg tatgagcgcg gtggtggtaa
     2521 cg
//
LOCUS       DATASH010000681         2267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3671115,
            whole genome shotgun sequence.
ACCESSION   DATASH010000681 DATASH010000000
VERSION     DATASH010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3671115"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="VHV99_18215"
     CDS             <1..591
                     /locus_tag="VHV99_18215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX3637380.1"
                     /translation="LLVERIMGTLNGWLSAEGAPPARLLEGADLDLAVARGAAYYGYV
                     RRGKGVRIRGGTARAYYIAIESAMPAVPGLEPPVQALCVAPFGMEEGTDAELPAQEFG
                     LVVGEPVHFRFFGSSVRRQDQVGTLLDFWGPEELQELEEIQATLPSAGRTAGEVVPVK
                     LHARVTEAGTLELEAVPRGTDERWKVEFDVRGGANA"
     gene            588..>2267
                     /locus_tag="VHV99_18220"
     CDS             588..>2267
                     /locus_tag="VHV99_18220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX3637381.1"
                     /translation="MTAMKRYSVGIDLGTSNTVLAYAEARHPEAGSQAIHVFEIEQLV
                     SLGEVAARPLLPSVRYHAAPGELSAGDLQLPWRTAKGSADGAESQPVVIGRLARTLGG
                     QVPGRLVASAKSWLSHASVDRTAPILPWGAADDVRKVSPVEASASYLAHVRAAWNQRF
                     PDAPLEAQDVVLTVPASFDEGARALTVEAAREAGLPTLKLLEEPQAAFYDWLFHHRES
                     LAAELADTRLVLICDVGGGTTDLTLIEVNLRDGEPELTRIGVGNHQMLGGDNMDLALA
                     HRVEARLPQAGGERTRLSAASLSQLVERCRGAKEQLLGPQAPESASITLLGAGSKLIG
                     GARTVQVTREEVEQIIVDGFFPAVASNERPGRARGAIVEFGLPYATDAAVTRHIAAFL
                     SRFAAQSRKALGSATPDSSDTGEGEDAPLPVPDTLLLNGGVFRAHALAQRLSSTLGTW
                     RGAALNVLHNDNPDVAVARGAVAYALARAGQAPKIGGGSARSYFLVLDEAGDGERDAL
                     PTQRGICLLPRGTEEGHEILIADRTFALRLGHPVQFHLVSSSADTVYQAGEL"
BASE COUNT          342 a          747 c          805 g          373 t
ORIGIN      
        1 ttgctggtcg agcggatcat gggcacgctc aatggctggc tcagcgccga aggtgcgccg
       61 ccggcacgcc tgctcgaagg cgccgatctc gatctcgccg ttgcgcgcgg cgcggcctat
      121 tacggctatg tgcggcgcgg caagggcgtg cgcattcgcg gcggcacggc acgcgcgtac
      181 tacatcgcga tcgaatcggc gatgcccgcc gtccccggtc tcgagccgcc tgtgcaggca
      241 ctgtgcgtcg caccgttcgg catggaggaa ggcacggacg ccgagttgcc ggcgcaggag
      301 ttcgggctgg tcgtcggcga gccggtgcat tttcgtttct tcggatcgtc ggtgcgtcgt
      361 caggatcagg tcggcacgct gctcgacttc tggggccccg aagaactgca ggaactcgag
      421 gaaatccagg cgacgctgcc ctccgccggc cgcaccgccg gtgaagtcgt tcccgtgaag
      481 ctgcatgcgc gcgtgacgga agccggcacg ctcgaactcg aagccgtgcc gcgcggcacc
      541 gatgaacgct ggaaggtcga gttcgacgtg cgcggcggcg ccaatgcatg acagcgatga
      601 agcgctatag cgtcggcatt gatctcggca ccagtaacac ggtgcttgcg tatgccgagg
      661 cgcgtcaccc cgaggccggc tcacaggcga ttcacgtctt cgagatcgag cagctggtga
      721 gtctcggcga agtcgccgcg cggccgctgc tgccttcggt gcgctatcac gcggcgccgg
      781 gcgagttgag cgcgggcgat ctgcagttgc cctggcgtac agccaagggt tctgccgatg
      841 gcgctgaaag ccagccggtc gtcatcggcc ggctcgcgcg cacgctcggc gggcaggtgc
      901 cgggacggct ggtggcgagt gcgaagagct ggctctcgca tgcctcagtc gaccgcaccg
      961 cgccgatcct gccgtggggc gccgccgacg acgtccgcaa ggtctcgccg gtcgaagcca
     1021 gcgcgagcta tctggcgcac gtgcgagcgg cctggaatca gcgctttccg gacgcgccgc
     1081 tcgaagcgca ggacgtcgtg ctgacggtgc ccgcttcctt cgacgaaggg gcgcgcgcgc
     1141 tgacggtgga agcggcgcgt gaagcgggcc tgcccacctt aaagctgctg gaggagccgc
     1201 aggccgcctt ctacgactgg ctgtttcatc atcgcgagag cctggcggct gaactcgcgg
     1261 atacccggct cgtgctgatc tgcgacgtgg gcggcggcac caccgacctc acgctgatcg
     1321 aagtcaacct gcgcgacggc gagccggaac tcacgcgcat cggcgtgggc aaccatcaga
     1381 tgctcggcgg cgacaatatg gatctcgcgc tcgcgcatcg ggtcgaggcg cgtttgccgc
     1441 aagccggtgg cgaacgcacg cgtttgtcgg cggcgagtct gtcgcaactg gtcgagcgtt
     1501 gccgcggcgc gaaggaacag ctgctcggcc cgcaagcgcc ggagtccgct tccatcacgc
     1561 tgctcggcgc gggctcgaag ctgattggcg gcgcgcgcac ggtgcaggtc acacgcgagg
     1621 aagtcgagca gatcatcgtc gacggattct ttccggccgt ggcctcgaac gaacggcccg
     1681 gcagagcgcg cggcgcgatc gtcgaattcg gcctgcccta tgccaccgat gcggcggtca
     1741 cgcgtcatat cgcggcgttt ctgagccgct tcgcggcgca gtcgcgtaag gcgctcggtt
     1801 ccgccacgcc cgattcctct gatacgggcg aaggcgagga cgcgcctttg ccggtgcctg
     1861 acacgctgct gctgaacggc ggcgtgtttc gcgcccacgc gctcgcgcag cgcttgtcca
     1921 gcaccttggg tacatggcgc ggcgcagcgc tcaacgtgct gcacaacgac aatcccgacg
     1981 tcgccgtggc gcgcggcgcg gttgcttatg ctctcgcccg cgcgggtcag gcgccgaaaa
     2041 tcggcggcgg ctcggcgcgc agttatttcc tcgtgctgga cgaagccggc gacggcgaac
     2101 gcgacgcctt gccgacgcaa cgcggcatct gcctgctgcc gcgcggcacg gaggaaggcc
     2161 atgagattct gatcgcggat cgcacgttcg cattgcggct cggacatccg gtgcaatttc
     2221 acctggtgtc gtcgagcgcg gacaccgtct atcaggcggg cgagctg
//
LOCUS       DATASH010000682         9804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6241236,
            whole genome shotgun sequence.
ACCESSION   DATASH010000682 DATASH010000000
VERSION     DATASH010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 9804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9804
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6241236"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1876)
                     /locus_tag="VHV99_18225"
     CDS             complement(<1..1876)
                     /locus_tag="VHV99_18225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018432265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX3637382.1"
                     /translation="MSGAVHHHALVAQELDVFACSLDGVNQIEASAGTGKTWNICALY
                     VRLLLEKNLNADQILVVTFTKAATAELHERIRGRLAELDRAIDTDDDGGDPFVRRLFE
                     TTLAPERGIDRETALKVVRRALRTFDQAAIHTIHAFCQRALQEAPFAAAMPFAFEMEA
                     DDAALRFEMAADFWREQVEPVAYAHPAFAAWLVAKRAGPASLDEQLARRLKKPLAQLR
                     WGKVDAGGSNADPQAGFDAACAVWQAERDAIVSLLGEAQDRLSRTSHKPDLVSAAIAA
                     WSDYFAQGDCHAPPPKAALKLTASALKKATKVKFEAPGHPFFEHAEALAAAVVAAEAA
                     QRARWLSLVETWLDYAPAELVARKRTRRVVSFDDLLSNLYRALAANPWLADALRTRYP
                     AALIDEFQDTDPLQFAIFSRIFAPAGPLFLVGDPKQAIYSFRAADLHTYLAARAAASA
                     CYTLAVNQRSTAPIVEACNRLFEANPQAFVLDGLDYQPVRAGERRRPPFSEPDASAGD
                     FRIWTLPQADAALTKREAQRAASEACAAEIVRLLRCAREGSVTIGDAPLAPGNIAVLV
                     QTHKQGSLIKRVLASWGVGSVELAQASVFATLDAEQIERVLAAVDTPGDLRRLRAALA
                     T"
     gene            complement(1873..5271)
                     /gene="recC"
                     /locus_tag="VHV99_18230"
     CDS             complement(1873..5271)
                     /gene="recC"
                     /locus_tag="VHV99_18230"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HEX3637383.1"
                     /translation="MLQLFYSNRYETLVSALLDDLAQAPSDPWAAQPVIVPSAAVRRR
                     LELDIAARQGICANVNFGYLAQWLWAQIGGVIVVPKHSPFAPDRLVWRCYRLLGEADE
                     GLPWNASPRLRTYLDAADASMRYELARRVATVLDHYLTYRPEWLLQWQKGGSIFASGA
                     AEDNGPRLVGASVAAREDERWQAALWRAVLAEVAQVAGNAQTPAAALPPAYRFLEEIG
                     TLDLEAISNAQWPEAVSVFALPTMPPLHVALLRALSRWVDVRLYVMNPCREFWFDVVS
                     AGRVEALDVAGQLDYQEVGHPLLAEWGRQTQAQLHMLHELTESAASGETGDFSENPEP
                     SWLAAVQNGILDLRAEVGTDTDTNELPLENGIEVHVCHSLSRQLEVLHDRLLGWFDEF
                     DDLQPSDVLVAVSDLAAAGPLIDAVFGTAPPGDTRRIPYRITGLPPSQANPVARLLLD
                     WLALPERSVGAPDLIEWLRVDAIAARYGIDASALEAAQEWLAAAGARRGLTPVEPTGE
                     NVPVARHTFADALTRLYLGYAMPEGGEPVDAWLPVEGADGSDAELLGRLSRFVDDIET
                     FAERCAIEQTPAEWTQLLLETLAQCFDGGVEFADSLAAVRDAIDKMGDAMQAGAEDVV
                     LPAAVVRGALTEALDDPARGGVPWGSVTFSSLTSLRGLPYRIVCLLGMDDGVLPSLAR
                     ADEFDLMAAFGKAGDRQRRDDERNLFLDLLLAARDRLFIAYTGRSIRDNAPLPPAALV
                     DELLDHLAHVSAGEGASPAEIEAARHAFIIDHPLQAFASDYFSSQRGLFTYDMDRAEL
                     ASLLAAPPHPAAAPFFDQPLPAEDTAAVAFDEFVRFWRHPARALLRDRLGIVLSDAQG
                     ELLDTEPFELDWAGRDALAERLLPVLLNSDSEDDDSMFDRVRRVAAASPELPGGATGA
                     VWRSRELGALRQLADSVRREVASGVERLPFVLDIVPRWPDRADIADTLFGTQDAALRK
                     AAEAPLQLHGTLNLLTETGQVIFRYAKPTARDYLSAWLAHLVYCAAQPNGPRRTVWHG
                     SGESFELTPVTTPLDELAPLAALFRAGRMLPLRFFPKSAWAKVSESDSAAQGVWINDR
                     VRGESDDPALRIALRGTPLTLDEPFGSLASIVFKPLVQHLRSAS"
     gene            5483..7993
                     /locus_tag="VHV99_18235"
     CDS             5483..7993
                     /locus_tag="VHV99_18235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family membrane protein"
                     /protein_id="HEX3637384.1"
                     /translation="MRYSVEIRKFLYSQYFYGGLRIAVGVSLPAVLCLIVFHDRELGF
                     TIATGALGACVVDMPGPLKYKHNEMLACTVIGFLSALATGIATVNPVALWCTVVPLTF
                     VLSLIVVYGNRWPQISFATLFMMIMTLEDHFTPMQALVNASWILLGGLWYTYWSTFVS
                     RWMMHRIEQQALAESVFACADYLLARAAFYDLDNDLDECYRNLVDKQISAVERQDAAR
                     DIVLRNLPKLKSGRLEPRRAMLFNLFINTVDLHELFVGTHVDYSVVRNTFGGSDLLVF
                     YRDLIRKSAADLEEIGLAVLQNQPPRTRVNVKAELRAIEFEIDVMRKQDLPAKNPEAY
                     SAISSTFRRIWSATRLIDKMRKSLSSDPSATETELRLDQALSRFVSSRRVPFGQIFSN
                     LTMASPSFRHALRVTIAVAVGFWLGRLLPLTNAYWIVMTTVIILKPGYSLTKQRNGQR
                     IIGTLIGCAASIALMIFVNEPHILLVVMFASMVMSYSLLLFNYTASVVFTSSYVLLMF
                     HLLAPGSMRIIGERAIDTVVGCAIAIAASHLFPYWEYRLMGKLVNNMISATRQYLEAS
                     WWWSGKPAAAVVATVTEGGAFAPAAAMADPALDFGKPVLAMANGGGNASGNAGPKAGA
                     SGGATADANAGANSNATASASSAGTTTVRNATAKPTGGASPAAAAAATALDRDYRYRL
                     ARKNVHVAFANLGQAFQRMMLEPKSAQKFVPELNGLLVRSHVLASQITVAAPLLRTSA
                     QQADSVSLQPLQRALSVIRDNLTEAEAGSAPPADQGEQAKQLSRELDAMVVETEKSPD
                     YSADTVRDMKLLAHQCKQMLAASALIRKDASVIRLPEE"
     gene            complement(8021..8230)
                     /locus_tag="VHV99_18240"
     CDS             complement(8021..8230)
                     /locus_tag="VHV99_18240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637385.1"
                     /translation="MNKPPRKKNPTFGIAVFIVVAVLLVIATLFYNAITEKREFDREN
                     APAPTISSEAAAAAAVAKPASGTPQ"
     gene            complement(8318..8800)
                     /locus_tag="VHV99_18245"
     CDS             complement(8318..8800)
                     /locus_tag="VHV99_18245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007740526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX3637386.1"
                     /translation="MGMDIIDRKLLELLQEDATMPIAELAQRVNLSQTPCWKRLQRLK
                     ETGVIRAQVALCDARKLGVGTTVFVAVRTNQHTEAWAQTFTRAVQEIPEVVEVYRMSG
                     ETDYLLRVVVSDIDDYDRVYKLLIAAVPLYDVSSSFAIEQIKYSTALPVRPSVVVEGR
                     "
     gene            8939..9583
                     /locus_tag="VHV99_18250"
     CDS             8939..9583
                     /locus_tag="VHV99_18250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007588476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HEX3637387.1"
                     /translation="MSQDLRSTPVTRSAAHAAQFAGTNETPSCAHHSAPLTHLCDALD
                     AIFDACVKFPEPSDSSFFARGMRIALAEAAANPDLLTSAQREGSAENYRRHLLAADPH
                     GRYAIAALVWLPRQASPVHAHHTWCGYAVLDGTLSETVFEWNGARHCASATRTQARHA
                     GAVSFVRGGRGGIHRLGNCSDAPAVSLHIYGVAGAQIATHVNDIVRAADAPALV"
BASE COUNT         1826 a         3250 c         3075 g         1653 t
ORIGIN      
        1 ctgtcgcgag cgcggcgcgc aaacgacgca gatcgcccgg cgtatcgacc gcggccagca
       61 cgcgttcgat ctgctcggcg tcgagcgtcg cgaacaccga ggcctgcgcc agttccacgc
      121 tgccgacgcc ccacgacgcc agcacacgct tgatcaggct gccctgcttg tgcgtttgca
      181 cgagcaccgc gatattgccc ggcgcaagcg gcgcgtcgcc gatcgtcacc gacccttcgc
      241 gtgcgcagcg caggagccgc acgatctcgg ccgcgcaggc ttcgctcgcc gcgcgctgcg
      301 cttcgcgttt ggtcaacgcg gcgtcagctt gcggcagggt ccagatgcgg aaatctcccg
      361 cgcttgcatc gggttcggag aaaggcggac gacgccgctc gccagcccgc accggctgat
      421 agtcgagacc gtcgagcacg aacgcctggg gattggcttc gaacagccgg ttgcaagcct
      481 cgacgatcgg cgcggtcgaa cgctgattga ccgcaagcgt gtagcacgcc gaagccgccg
      541 cgcgcgccgc cagataagta tgcagatccg ccgcgcggaa actgtagatc gcctgcttcg
      601 gatcgccgac cagaaacagc ggaccggccg gcgcgaagat gcggctgaag atcgcgaact
      661 gcaacggatc ggtatcctga aactcgtcga tcagcgcagc cggataacgc gtgcgcaacg
      721 catcggcgag ccaagggttg gcagcgagcg cgcgataaag attggacagc aggtcgtcga
      781 acgacaccac cctgcgcgtg cgtttgcgcg ccaccagttc ggcgggggcg tagtcgagcc
      841 atgtctcgac gagcgagagc cagcgcgccc gttgcgcggc ctcggccgcg accacggccg
      901 cagccagcgc ttcggcgtgc tcgaagaacg gatgcccggg cgcttcgaac ttgaccttgg
      961 tggccttctt caacgccgaa gccgtcagct tcaaggccgc ctttggcggt ggcgcatggc
     1021 aatcgccctg ggcgaagtag tcgctccacg cggcaatcgc ggcgctcacg aggtcaggct
     1081 tgtgcgaggt cctgctcaaa cggtcttgcg cttctccaag cagactcacg atcgcgtcgc
     1141 gctccgcctg ccagactgcg caagcagcat cgaaacccgc ctgcgggtcg gcattgcttc
     1201 cgcccgcatc gaccttgccc cagcgcaact gcgccaacgg cttcttcagg cgccgtgcga
     1261 gttgctcgtc gagcgacgcg ggaccggcgc gtttggccac cagccaggca gcgaaagcag
     1321 gatgcgcata ggccaccggt tccacttgct cgcgccagaa atcagccgcc atttcgaagc
     1381 gcaaggcagc gtcgtcggct tccatttcga aggcgaacgg catggccgcg gcgaacggcg
     1441 cctcctgcaa ggcacgctga cagaacgcgt gaatggtgtg aatcgcggcc tggtcgaacg
     1501 tgcgcagcgc gcggcgtacg actttcaacg cggtctcgcg atcgatgccg cgctccggcg
     1561 caagggtcgt ttcaaagaga cgccggacga acggatcgcc gccgtcgtcg tccgtatcga
     1621 tcgcccgatc cagttccgcg agccgcccgc gaatccgctc gtgcaactcg gcggtcgccg
     1681 ccttcgtaaa ggtgaccacg agaatctgat cggcgttcag gttcttttcg agcagcagcc
     1741 gcacgtacaa cgcgcagatg ttccaggtct tgccggttcc ggccgacgcc tcgatctgat
     1801 tgacgccgtc gagcgaacag gcgaagacgt cgagctcttg cgcaaccagt gcgtgatggt
     1861 gaacggcgcc gctcatgatg cgctccgcag atgctgcaca agcggtttga agacgatcga
     1921 ggcaagactg ccgaacggtt cgtcgagcgt aagcggcgtg ccgcgcagcg cgatgcgcaa
     1981 agcagggtcg tcggattcgc cgcgcacacg atcattgatc cacacgccct gcgccgcgga
     2041 atcgctctca ctgaccttcg cccacgcgct cttcgggaaa aagcgcagcg gcagcatgcg
     2101 ccctgctctg aacaacgcgg caagcggcgc cagttcatcg agcggcgtgg tgaccggcgt
     2161 gagttcgaaa ctctcgccgc tgccatgcca aaccgtgcgg cgcgggccgt tcggctgcgc
     2221 cgcgcaatag accagatggg caagccatgc cgacagatag tcgcgcgcag taggtttggc
     2281 gtaacggaaa atcacttgtc ctgtttcggt cagcaaattc aaggtgccgt gcaattgcag
     2341 cggggcttcg gccgctttgc ggagcgccgc gtcctgagtg ccgaagagcg tatcggcgat
     2401 gtcggcacga tcaggccagc gcggcacgat atcgagcacg aacggcaaac gctcgacacc
     2461 cgaagcgact tcgcggcgca cgctatcggc gagttggcgc agcgcgccga gttcacgcga
     2521 gcgccatacc gcgccggtcg ccccacccgg caactcggga ctggccgcgg cgacacggcg
     2581 cacgcggtcg aacatgctgt cgtcgtcttc gctatcgcta ttcagcagga caggcaggag
     2641 ccgttccgcc aaggcatcgc ggccggccca gtcaagttcg aacggttcag tgtcgagcaa
     2701 ttcgccctgc gcatccgaca acacgatacc caaccggtcg cgcaacaacg cacgcgccgg
     2761 atgacgccag aaacgcacga attcgtcgaa ggcaacggcg gccgtgtcct cggcgggcaa
     2821 cggttgatcg aagaacggcg cggctgccgg atgcggcggc gcggccagca aactcgccag
     2881 ttccgcgcga tccatgtcgt aagtgaagag gccgcgctgt gacgagaaat agtccgacgc
     2941 gaacgcctgc agcggatgat cgatgatgaa cgcgtgccgc gcggcttcta tttccgcggg
     3001 actcgcccct tcccccgccg acacgtgcgc gaggtgatcg agcaattcgt cgaccagcgc
     3061 ggccggcggc aacggcgcat tgtcgcgaat gctgcggccc gtataggcga taaacagccg
     3121 gtcgcgcgca gccagcagca gatcgaggaa cagattgcgc tcatcgtcgc ggcgctgccg
     3181 gtcccctgcc ttaccgaacg cggccatcag gtcgaattcg tcggcgcggg cgagactcgg
     3241 caacacgccg tcgtccatgc cgagcagaca gacgatgcga tacggcaagc cgcgcaagct
     3301 ggtcagcgac gaaaatgtca cgctacccca aggcacgccg ccgcgcgccg ggtcgtcgag
     3361 cgcctcggtc agcgcgccgc gcaccacggc ggccggcagc acgacgtctt ctgcgcccgc
     3421 ttgcatcgcg tcgcccatct tgtcgatggc gtcgcgcact gccgccagcg agtcggcaaa
     3481 ctccacgccg ccgtcgaaac actgcgcgag cgtttcgagc aacagctgcg tccattcggc
     3541 gggtgtctgc tcaatcgcgc agcgctctgc gaatgtctcg atatcgtcga cgaaacgcga
     3601 caggcgcccg agcaactccg cgtcagaacc gtcggcgcct tcgaccggca accacgcgtc
     3661 gaccggctcg ccgccttccg gcatggcgta gccgaggtac agacgggtca acgcatcggc
     3721 gaacgtatga cgcgcgacag gcacgttttc gcccgtcggc tcgacgggtg tcaatcctcg
     3781 acgcgcgccg gctgccgcga gccattcctg cgcggcttcg agggcgctgg catcgatccc
     3841 gtaacgcgca gcgattgcat cgacccgcag ccattcgatc agatccggcg cgcccacgct
     3901 gcgctcgggc aacgccagcc agtccagcag caaacgcgcg accggattag cctgcgaagg
     3961 cggcaaacca gtgatccgat acgggatgcg gcgagtgtcg cccgggggcg cggtgccgaa
     4021 caccgcatcg atcaatggcc cggcggccgc gagatcggac acggcaacca gcacgtcgga
     4081 cggctgcaga tcgtcgaact cgtcgaacca gccgagcagg cggtcatgca acacttcgag
     4141 ctgccgcgag aggctgtgac acacatggac ttcaatgccg ttctcaagcg gcaattcgtt
     4201 ggtgtccgtg tctgttccca cttccgcccg cagatcgagg atgccgttct gcacggcggc
     4261 gagccagctc ggttctggat tctcgctgaa gtcgcccgtc tcgccggatg cagcgctctc
     4321 ggtcagctca tgcagcatgt gcagttgcgc ctgagtctgg cggccccact cggcgagcag
     4381 cggatgaccc acctcctgat aatcgagctg gcctgcaaca tcgagcgcct cgacgcggcc
     4441 ggcactgacg acgtcgaacc agaactcgcg gcacgggttc atcacgtaga ggcgcacatc
     4501 gacccagcgc gacagcgcgc gcagcaacgc gacgtgcagc ggcggcatgg tcggcagcgc
     4561 aaacacgctg accgcttcgg gccactgcgc gttcgagatg gcttccagat cgagcgtgcc
     4621 gatttcttcc agaaagcgat aagcaggcgg caacgcggcc gcgggcgttt gcgcgttacc
     4681 tgctacttgc gccacttcgg caagcacggc gcgccacaat gccgcttgcc aacgctcgtc
     4741 ctcacgcgcc gcaacgcttg cgccgacgag acgcggaccg ttgtcctccg cggcgccgct
     4801 cgcgaagatc gagccgccct tttgccattg cagcagccat tccgggcgat aggtcagata
     4861 gtggtcgagc accgtggcca cgcgacgggc cagttcgtag cgcatcgacg cgtccgccgc
     4921 gtcgagataa gtgcgcagac gcggcgaggc gttccagggc agaccttcat ccgcctctcc
     4981 cagcagccgg tagcagcgcc agacgagccg gtccggcgcg aacggcgaat gcttcggcac
     5041 tacgatcacg ccgccgattt gcgcccacaa ccactgcgcg aggtaaccaa agttgacatt
     5101 cgcgcaaatg ccctggcgcg ccgcgatgtc gagctcgagc cggcgccgca ccgccgcgct
     5161 cgggacgatc acgggctgcg cggcccaggg atcggacggc gcttgtgcca ggtcgtcgag
     5221 cagcgcgctg acaagggttt cgtagcggtt cgagtagaag agctggagca tggattccgg
     5281 ggaagcatgc ggcgcgagcg gaacgcccgg gccgcgacag acattgaagc gacagcataa
     5341 caaacgcgct cccggggcag aacctggcca aacggacgag gtcagatcag acacaaaatc
     5401 agttagactc gacggcagtt tgggtctgct cagggtcggc ctctccaacc gcgtttctca
     5461 gtgaatggaa tccaggcagt cgatgcgcta ttcggttgaa ataagaaaat tcctctacag
     5521 ccagtacttt tatggcggcc tgcgcatcgc agtcggcgtg tcgttaccgg ccgtattgtg
     5581 tttgatcgtg tttcacgatc gcgagctcgg cttcacgatc gcgaccggcg cactcggcgc
     5641 atgcgtggtc gatatgcccg gcccgctcaa gtacaaacac aatgagatgc tggcgtgcac
     5701 ggtaatcggc ttcctgtccg cgctcgccac cggtatcgcc acggtcaacc cggtggcgct
     5761 gtggtgcacg gtcgtgccgc tcacgttcgt gctctcgttg atcgtcgtgt atggcaaccg
     5821 ctggccgcag atcagctttg ccacgctgtt catgatgatc atgacgctgg aagaccattt
     5881 cacgcccatg caggcgctcg tcaatgcgtc gtggattctg ttgggcggcc tctggtacac
     5941 ctattggtcg accttcgtga gccgctggat gatgcatcgc atcgaacagc aggcactcgc
     6001 cgaaagcgtg ttcgcgtgtg cggactatct gctcgcgcgc gccgccttct acgatctcga
     6061 caacgatctc gacgagtgct atcgcaatct ggtcgacaag cagatctcgg cagtggaacg
     6121 tcaggatgcc gcgcgcgaca tcgtgctgcg caatctgccg aagctgaaaa gcggccggct
     6181 cgaaccgcgc cgggccatgc tgttcaatct gttcatcaat accgtcgatc tgcacgaact
     6241 gtttgtcggc acgcacgtcg attactcggt ggtccgcaat acgttcggcg gctccgacct
     6301 gctggtgttc tatcgcgatc tgattcgtaa atccgcagcc gatctcgaag agatcggact
     6361 cgcggtattg caaaaccagc cgccgcgtac gcgcgtgaat gtcaaggccg aattgcgcgc
     6421 catcgagttc gagatcgatg tgatgcgcaa gcaggatttg cccgccaaaa atcctgaggc
     6481 ttattcggcg atatcgtcca ccttccggcg tatctggagc gccacgcggc tgatcgacaa
     6541 gatgcgcaag agcctgtcga gcgaccccag cgcgaccgag accgaactgc gcctcgacca
     6601 ggcgctctcc cgcttcgtgt cgagccggcg cgtaccgttc gggcagatct tctcgaatct
     6661 gaccatggcc tcgccgagct tccgccatgc gctgcgcgtg acgattgcgg tcgcggtggg
     6721 tttctggctc ggccgtctgc tgccgctcac caatgcgtac tggatcgtga tgaccaccgt
     6781 catcattctg aagcccggct actcgctgac caagcagcgc aacgggcagc ggatcatcgg
     6841 cacgctgatc ggctgcgcgg cgagcatcgc gttgatgatc ttcgtgaacg agccgcacat
     6901 cctgctggtg gtgatgttcg cgtcgatggt gatgagctat agcttgctgc tgttcaatta
     6961 cacggcgagc gtggtgttca cctcgtcgta tgtgctgctc atgttccatc tgctcgcgcc
     7021 gggcagcatg cggatcatcg gcgaacgcgc gatcgacacg gttgtcggct gcgcgatcgc
     7081 gatcgccgcg agccacctgt tcccctactg ggaataccgg ttgatgggca agctcgtcaa
     7141 caacatgatc agcgcgacgc ggcagtatct ggaagcgagt tggtggtgga gcggcaagcc
     7201 ggccgccgcg gtggtcgcca cggtgactga gggcggtgca tttgcgcctg ccgcggcgat
     7261 ggccgatccg gcgcttgatt tcggcaagcc ggtgctcgcg atggcaaacg gcgggggaaa
     7321 tgcgagcggc aacgctgggc caaaagctgg cgcaagcgga ggtgcgaccg ctgacgcaaa
     7381 cgccggtgca aactcaaatg cgaccgcaag cgcaagcagc gccggcacca ccacggtaag
     7441 gaacgcaacc gccaaaccga ccggcggcgc atcgcctgcc gccgcagccg ctgcaacggc
     7501 gctcgatcgc gactatcgct accggctcgc acgcaagaac gtccatgtgg cgttcgccaa
     7561 tctgggtcag gcgttccagc gcatgatgct cgaaccgaaa tccgcgcaga agttcgtgcc
     7621 tgaattgaac ggcctgctgg tgcgcagcca cgtgctcgcc tcgcagatca ccgtggccgc
     7681 gccgttgctg cgcacctctg cccaacaggc cgacagcgtc tcgctacagc cgttgcaacg
     7741 cgccctgagt gtgatccgcg acaacctgac cgaagccgaa gccggcagcg cgccgcccgc
     7801 cgatcagggc gagcaggcca agcaattgtc gcgtgagctg gatgcgatgg tggtcgaaac
     7861 ggagaaatcg ccggactact cggcggacac cgtgcgcgac atgaaattgc tggcccacca
     7921 gtgcaagcag atgctggcgg cgtccgcgct gattcgcaaa gatgcgagcg tgatccgcct
     7981 gccggaagag tgaaacgact gaagcgcaga tcggcccgtc ttattgcggc gtgccgcttg
     8041 cgggcttcgc tacggcagcc gcagccgccg cttccgatga aatcgtcggc gccggcgcgt
     8101 tctcgcggtc gaattcgcgc ttttccgtga tggcgttata gaagagcgtg gcgatcacca
     8161 gcagcaccgc gaccacgatg aaaacggcaa tgccaaaagt cggattcttc ttgcgcggcg
     8221 gtttgttcat gaggcgggct cggttcgcga ggcagtcgcg gatgatgtct gggaaggcgg
     8281 cattctacgc cccctgagct tgcacatggc ttgcatgtca acgcccttcc accaccacgg
     8341 atggccgcac cggcagcgcc gtcgaatact tgatctgctc gatcgcaaag ctcgaactca
     8401 cgtcatacag cggcacggcg gcgatcaaca gtttgtacac gcgatcgtag tcgtcgatat
     8461 cggacaccac cacgcgcagc aggtaatccg tttcgccgct catccggtac acctccacga
     8521 cctccggaat ctcctgcacc gcacgcgtaa aagtctgcgc ccaggcctcg gtgtgctggt
     8581 tagtgcgcac cgcgacaaac acggtcgtgc ccacgccgag cttgcgcgca tcgcacagcg
     8641 ccacctgggc gcgtatcacg cccgtctctt tcagacgctg cagccgcttc cagcaaggcg
     8701 tttgcgacag attcacgcgt tgcgccagtt cggcgatcgg catggtggcg tcttcctgca
     8761 gtagctccag aagcttccga tcgatgatat ccattcccat cgcgacactc ccataggaaa
     8821 ttattctact tttcagactt caaggggaat tatatggaaa ctctttctcc agtcaaacgg
     8881 gtataaatgt ggctatccat aaatagccga taagcagcca taccgctgcg cagcgatcat
     8941 gagccaagac ctccgttcaa cacccgtcac ccgttccgcc gcccacgctg cccagtttgc
     9001 cgggacgaat gaaacgccct cgtgcgcgca tcacagcgcg ccgcttacgc acctatgcga
     9061 cgcgctcgac gcgatcttcg acgcctgcgt gaaattcccc gaaccgtcgg attcaagctt
     9121 cttcgcgcgc ggcatgcgca tcgcactggc cgaggccgcg gcgaatcccg acttgctcac
     9181 atcagcgcaa cgcgaaggct ccgctgaaaa ctaccggcgt cacctgctgg ccgcggatcc
     9241 gcacggccgc tatgcgatcg ccgcgctggt ctggctgccg cgccaggcga gcccggtaca
     9301 tgcgcaccac acatggtgcg gctacgcggt gctcgacggc acgctgagcg aaacggtctt
     9361 cgaatggaat ggcgcacggc actgcgcaag cgctacccgg acacaagcgc gccatgccgg
     9421 cgccgtgtcg tttgtacgcg gcggcagggg cggcattcat cggttgggca attgcagcga
     9481 cgcgccggcc gtctcgctgc atatttacgg tgtcgcgggc gcgcagatcg ccacacatgt
     9541 caacgacatc gtgcgcgccg ccgacgcacc ggcgctcgtc tgaaaccggt aggtcctgcc
     9601 taaagacgcg aaccgcgggc gcgaaaagcg gacgcggacc gcgccaaggt ccgctagcct
     9661 cgcctcgcat ccagcacaaa tcagctttta ccgcccgctt ctccggccgt cggaatctgc
     9721 ggcggaatcg gcgcggtctg cccggtcggc ggcggcgccg gacgcggctc gtgcgacacg
     9781 tcgcgcgcca ccgccatcgc agcg
//
LOCUS       DATASH010000683         5644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6639018,
            whole genome shotgun sequence.
ACCESSION   DATASH010000683 DATASH010000000
VERSION     DATASH010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5644
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6639018"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..779)
                     /locus_tag="VHV99_18255"
     CDS             complement(57..779)
                     /locus_tag="VHV99_18255"
                     /inference="COORDINATES: protein motif:HMM:NF015437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer binding domain-containing protein"
                     /protein_id="HEX3637388.1"
                     /translation="MSQNLSEWWSDLFLELQTCEQIDAAFSILSRASLDLGFSYCAFG
                     YVNRVPFCSRNIVIKSSYPERWVSRYREKEYLRVDPIAKLESGDLTPVIWGDRLFADA
                     KHLWDEARSAGLAIGISQPCWDARGQFGVYSLAREGTPVGFSEIKTLRPFLLALGQLS
                     MSCFSRIADQQSSTATEAKLTQREIEILRWTADGKSAKGISNALDISVDTVNFHLKNC
                     KRKLNAQSKASAAAYAIAYGLL"
     gene            1387..1548
                     /locus_tag="VHV99_18260"
     CDS             1387..1548
                     /locus_tag="VHV99_18260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637389.1"
                     /translation="MAQSNSNIFDLPVFDPAQDWIALEEHRAARVESTAGGTHSKSGA
                     MHAAPFSRL"
     gene            1560..2807
                     /locus_tag="VHV99_18265"
     CDS             1560..2807
                     /locus_tag="VHV99_18265"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3637390.1"
                     /translation="MARFLQRWIVLRSKGFGALACRDFRLFVFGNSLSLIGMWVQRVG
                     AGWLVWEISHSSVWLGVLALADLFPTVFIAPIAGLMADRCDPRRVMLYCQWVAMAMAV
                     LQACLILGDASSMEWLLVVTLVTGIAGALCQPTRLSWASTLVPQQQLGSAVALTSLCF
                     SLARFVGPAVAGALLLKLDPSALFFVSALGYAVFIAAIVRIPASPRLREDMPPLHWRR
                     DVTDGFRYLASDAPLRAIFLLLIACAVCLRGIPDMAPAIADAMVQRGAQGFALLVAAA
                     GAGALAGGIWISGRDTTAPLARLIERHVLAASLCGLGLAFTQHFPVALVLFFALGFSV
                     VVTGIGAQTLIHLSVPQAMRGRVLALYGVTYRGGPALSALWLGACAPLWGLRVALGAG
                     AVVCMAAYCATKLRARYGDLPAR"
     gene            complement(2942..4447)
                     /locus_tag="VHV99_18270"
     CDS             complement(2942..4447)
                     /locus_tag="VHV99_18270"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEX3637391.1"
                     /translation="MLDILPRGSAPANDAPKEVPLGDDSEMFELAPVSLWLEDFSGVR
                     VLFNAWRAQGVTDLRAHFAEDPSRVAECAHSIRVIKVNQKTLTQFEAADFEALTSNLA
                     SVFRDDMLKTHLEELCQLWAGQTQFTSQTVNYTLGGRRLDVLLKGAVLPGHEDSWDRV
                     LVSVEDITELEGARHRVTLAEQYVRGLFEYSPVSLWVEDFSAVKRLLDEARAAGISDF
                     RVFTDVHPEFVERCMQEIHVLDVNQHTLAMFTAKDKKMLLARLSEVFRDDMRPHFREQ
                     LIDLWEGKLFQQREVLNYSLEGNEVHVHLQFSVLPGHERNWDLVLVALTDITARKKAE
                     AYLEFLGKHDVLTKLRNRSFYVDELNRLERKGPWPVTIIMADLNGLKRVNDQLGHAAG
                     DALLRRAGEVLAKATDAPFHAARIGGDEFAILLPDTDERGGAAMIDAIHQLVDLNNQF
                     YPGSLLSFSMGAATCQRGDRLEAGVQRADLLMYEEKRGHYANRPSADGSGD"
     gene            4724..>5644
                     /locus_tag="VHV99_18275"
     CDS             4724..>5644
                     /locus_tag="VHV99_18275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006028756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEX3637392.1"
                     /translation="MKPSLLVLIPLKDASRAAVENAFDVVHAADATQRAAAIVAHGET
                     IRAVLTNGTIGLTAAEIDQMPQLEFVSALGAGYENLPLEHARERGIVLVNGSGTNADC
                     VADHAFALLLAVVRDVPLLDQETRKGAWRETVPMCPNVSGKRLGIVGLGNIGEKVARR
                     GAGFDMEIGYHNRKAREGSSLRYFDSVEGLAQWCDFLVVATPGGAGTRHLIGKAVLDA
                     LGPDGFVVNVSRGSVVDTAALAEALTAGAIAGAGLDVYEGEPHPPEALLALRNVVLTP
                     HVGGRSPEAIRASVDNFLANARRHFAGEPVL"
BASE COUNT         1028 a         1611 c         1749 g         1256 t
ORIGIN      
        1 aggctcagcc gttccattgt ggtgaatacg atctcttcca taagtcagct cggtagctaa
       61 agtaatccgt aggcaatggc ataggctgct gcactggcct tcgactgggc attaagtttt
      121 cgcttgcagt ttttcagatg aaaattcacc gtatcgaccg atatgtcgag agcattcgag
      181 atgcctttgg ctgactttcc gtcggcggtc caacgaagga tttcaatttc gcgttgcgta
      241 agtttggctt cggtggcggt gctgctctgc tgatcggcaa tccggctgaa gcacgacatc
      301 gacaactgac cgagcgcgag gagaaaaggc cgcagggtct tgatctcact gaagcctacc
      361 ggcgtacctt ctctcgccag cgaataaacc ccaaactggc cgcgagcgtc ccagcaaggt
      421 tgtgaaatgc cgattgcgag tccggccgat ctggcttcgt cccataagtg ttttgcgtca
      481 gcaaagagac ggtcgcccca tatcaccgga gtcagatcgc ccgattcgag tttcgctatg
      541 ggatctactc tcaaatattc cttttctcga tatctcgaca cccatcgctc gggataagag
      601 cttttgatta cgatatttct ggaacagaaa ggcacgcgat ttacgtagcc gaacgcacaa
      661 tacgaaaatc ccagatccag agaggcgcgc gacaaaatag aaaacgcggc gtcaatctgt
      721 tcgcaggttt gtagttcgag gaatagatcg ctccaccatt cagacaggtt ttgggacatc
      781 acttgactcc gctatgtaag tttgtttgct gactgttcct taaataggtg gttctcgctc
      841 catagggtat gtactaacga taagctggta atagacatca tgtctgaaat tactggttat
      901 atcgtaaatc ataaactcgt agcgtttagc cgaaaggtac aatttcctac caaattttgg
      961 ctgtgacgta taggcgcatg ggcgccggtt ccacggtctc gccgcacgga gatggatatt
     1021 cccagaatga ataatcgaat ctgcggatga cattcctatt tttagaataa tttattcaga
     1081 cggaagccga atcagcgagc acgagatctt cccgagcgtg atgatccgtt gattaacaaa
     1141 gagaacggtc tgatgatgtg atacggattt ttcgtgatga gagtataggc agtaaaaaaa
     1201 tcacgtgcgg tcgtgaaagt acggagatcc tttggcgcgc atctattctt aaaacgtctg
     1261 atcgggaggc taccagtagc gggaggtgtt ttgtgattgg ttttatgtaa ttgtttgcgc
     1321 tttcggattg atgtcggcgg atcgataata aatcgacatt tatatttaac ctgaaaggag
     1381 tgtgctgtgg cgcaatcaaa ttcaaatatt tttgacctgc cagtttttga tcccgctcag
     1441 gactggatcg cgttagaaga gcatcgggca gcgcgagttg aatccactgc cggcggaaca
     1501 cactcgaagt ccggtgcgat gcatgccgcg ccgttctcgc gcctctgagc agaatgccga
     1561 tggcgcgctt ccttcagcgg tggatcgtac tcaggagcaa gggtttcggc gcgctcgcat
     1621 gccgggactt ccggctgttc gttttcggta acagcctgtc gttgatcggc atgtgggtgc
     1681 agcgggtcgg tgccggctgg cttgtgtggg agatcagtca ttcgagcgta tggctcggcg
     1741 tgctcgccct cgccgatctt ttcccgacgg tgttcatcgc accgattgcc ggcctgatgg
     1801 cggatcgttg cgacccgcgc cgcgtcatgc tctattgtca gtgggtcgcg atggcgatgg
     1861 ccgttttgca ggcctgtctg attctcggtg acgccagttc gatggagtgg ctgctcgtgg
     1921 tgacgctggt aacgggtatc gccggtgcac tgtgtcagcc tacgcgcctc tcgtgggcgt
     1981 cgacgctggt gccgcaacaa cagcttggct cggcagttgc gctcacctca ctgtgcttca
     2041 gcctggctcg ctttgtgggg ccggcggtgg cgggcgcgct attgctgaag ctcgatcctt
     2101 cggcattatt cttcgtcagc gcgctgggct atgccgtctt catcgccgcc attgttcgca
     2161 ttccggcgag cccgcgtttg cgtgaagata tgccgccctt gcattggcga cgcgacgtca
     2221 cggacggatt tcgctacctc gcatcggacg cgccgttgcg ggcaattttc ctgcttctga
     2281 tcgcgtgcgc cgtctgcctt cgcggtatcc ctgacatggc gccggccatt gcggacgcta
     2341 tggtgcagcg gggtgcacag ggtttcgcgt tgctcgtcgc cgcagcgggg gccggtgcgc
     2401 ttgcgggtgg catctggatc agtggccgcg acacgacggc gccgctagcg agattgatcg
     2461 agcggcacgt gttggctgct tcgctgtgcg gactcggatt ggcattcacg cagcatttcc
     2521 cggtagcgct ggtcctgttc tttgcgctcg gtttctcggt ggtcgtgact ggcatcggcg
     2581 cacagacgct gattcaccta tcggttccgc aggcaatgcg cggacgtgtg cttgcgttat
     2641 acggcgtgac ctatcgtggc ggaccggcgc tcagcgcgtt gtggctcggc gcgtgtgcac
     2701 cgctttgggg gctgcgcgtg gcgttgggcg cgggcgccgt ggtatgcatg gcggcgtact
     2761 gcgccacgaa gcttcgcgct cgttacggcg atttgccggc gcgatagcgg gttcctcggc
     2821 gtctcgtcag cctggccatt tagcaggttg ccggccgtca cgacgcaaac cggtataccg
     2881 ataccgggat gatcgggcac ctggttgcgg ctgcgcgcgg tggacaggat gcaacatttc
     2941 atcagtcgcc cgaaccgtct gcagagggac ggtttgcata gtgcccgcgc ttttcctcat
     3001 acatcagcag atcagcgcgt tgcacgccgg cctcgagccg gtcgccgcgc tgacacgtcg
     3061 ccgctcccat cgaaaagctc agcagcgagc ccggatagaa ctggttgttc agatcgacca
     3121 gctggtggat cgcatcgatc atcgcggcgc cgccgcgctc gtcggtgtcc ggcagcagga
     3181 tggcgaactc gtctccgccg atacgcgccg cgtgaaaagg cgcgtccgtc gccttggcaa
     3241 ggacttcgcc ggcccgccgc aacaaggcgt cgccggccgc gtggccgagc tgatcgttca
     3301 cgcgtttgag gccgttcaga tcggccatga tgatcgtaac cggccacgga cccttgcgtt
     3361 ccagccggtt cagttcatcc acatagaacg agcggttgcg cagcttggtc agcacgtcat
     3421 gcttgccgag gaactccagg taagcttcgg ccttcttgcg cgccgtgatg tcggtgaggg
     3481 ccaccagcac caggtcccaa ttgcgttcat ggccaggcag tacggagaac tgcaggtgca
     3541 cgtgcacctc gttgccctcg agcgaatagt tgagcacttc ccgctgctgg aacagcttac
     3601 cttcccacag atcgatcaac tgctcgcgga aatgcggccg catgtcgtcg cggaagactt
     3661 cggacaggcg cgccagcagc atttttttgt ctttcgccgt gaacatggcg agcgtgtgct
     3721 ggttcacgtc gagtacgtgg atttcctgca tgcagcgctc gacaaactcg ggatgcacgt
     3781 cggtgaacac ccgaaaatcg ctgatgccgg ccgcgcgcgc ttcgtcgagc agacgcttga
     3841 ccgcgctgaa atcttccacc cacagcgaca ctggcgaata ttcgaacagg ccgcgcacat
     3901 actgttcggc aagcgtcacg cgatgccgcg cgccttcgag ttcggtaatg tcttcgacgg
     3961 agaccagcac gcggtcccag ctgtcttcat ggccgggtag cacggcgcct ttgagcagca
     4021 cgtcgaggcg ccgcccgcca agggtgtagt tgaccgtctg actcgtgaac tgggtttgcc
     4081 cggcccacaa ttggcatagc tcctcgagat gggtcttaag catgtcgtcg cggaacacgg
     4141 aggcgagatt gctggtgagc gcctcgaaat cggcggcctc gaattgcgtc aacgtcttct
     4201 gattgacctt gatgacgcga atgctgtgcg cgcattcggc gacccggctc ggatcctcgg
     4261 cgaaatgggc gcgcagatct gtcacgccct gggcccgcca cgcgttgaat agcacgcgca
     4321 cgccgctgaa gtcttcgagc cagagtgaga ccggtgcgag ttcgaacatt tcagagtcgt
     4381 cccccagggg gacttccttc ggggcgtcgt tggcgggcgc tgagccgcgc gggagaatgt
     4441 ccagcatgag attcccgtgg tcggagttcg ggctatttta atgcggcttg gggccggctc
     4501 aaaagaaaga cgcttgaggc gccccgttgt gccggtccat tgaagaagat aacggcgggg
     4561 tttcggacgg gcttaagctg catgccaacg gttccgctgc cgtcgcgcaa ccggccagtt
     4621 cgcccgcgag cgcttgccag cgggactgcg ggactgcggg agccttgtta cactcacgcg
     4681 tcgtgtttcc cgtccgtcac cgctcaaaga aaaaacgccc ctcatgaagc catccctgct
     4741 ggttcttatc cccttgaaag atgcaagccg cgccgctgtc gagaacgctt tcgacgtcgt
     4801 ccatgccgcc gatgctaccc agcgggccgc cgcgatcgtc gcgcatggcg agacgatccg
     4861 tgccgtgctg accaacggca cgatcgggct gacagccgct gaaatcgacc agatgccgca
     4921 gctcgagttt gtcagcgcgc tcggtgccgg ctatgaaaac cttcctctgg agcacgcccg
     4981 cgagcgcggc atcgtcctcg tcaacggctc gggcaccaat gccgattgcg tggccgatca
     5041 cgcattcgcc ttgctgctcg cggtggtgcg cgatgtgccg ctactcgatc aggagacccg
     5101 caagggcgcc tggcgcgaaa cggtgccgat gtgtccgaac gtgtccggca agcggctcgg
     5161 tatcgttggg ctcggcaata tcggcgagaa ggtcgcgcgg cggggtgccg gattcgatat
     5221 ggaaatcggt tatcacaacc gcaaagcgcg tgaaggctcg tcgctgcgct atttcgacag
     5281 cgtggagggg ctcgcgcagt ggtgcgattt tctggtggta gcgacgccgg gcggcgccgg
     5341 cacgcgtcat ctgattggca aggcagtgct ggacgcactc ggaccggacg gtttcgtagt
     5401 caacgtctcg cgcggcagtg tggtggatac cgctgcctta gcagaagcgc ttaccgccgg
     5461 cgcgattgcc ggcgcgggtc tcgacgtcta cgagggcgag ccacatccac ctgaagcctt
     5521 gctggcgctg cgcaacgtgg tcctgacgcc gcacgtgggc ggccgctcgc cggaggcgat
     5581 cagggcctcg gtcgacaatt tccttgcgaa cgcccgacgc cattttgcgg gcgaaccggt
     5641 attg
//
LOCUS       DATASH010000684         2870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4858022,
            whole genome shotgun sequence.
ACCESSION   DATASH010000684 DATASH010000000
VERSION     DATASH010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4858022"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..1259)
                     /locus_tag="VHV99_18280"
     CDS             complement(39..1259)
                     /locus_tag="VHV99_18280"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX3637393.1"
                     /translation="METYDIAVIGAGAAGMMCAAVAAQNGRRVVLIDHAERLAEKIRI
                     SGGGRCNFTNINAGPANFLSANPHFCRSALARYTPQDFLALLKRHRVDWHEKHKGQLF
                     CDDSSESIIQLLKSECDAGGVTWRRPVTVDTIRHDGARFFLDSNAGSISSAALVVATG
                     GLSIPKIGATDFAYRLAKQFGHKLIDTRPALVPLTFAPADWEPFAALSGLSVPVELSA
                     GAGRGRGEFSEDLLLTHRGLSGPGVLQISSYWNPAEPIRIDLLPGVDATSELIAAKRE
                     SRKQVGTFLAERVPARLAQTWLDVQRVAADARLADLPDKTLRQIGESLSRWTLTPNGT
                     EGYRKAEVTRGGVDTRELSSTTMMSSRVTGLYFIGEAVDVTGWLGGYNFQWAWASGVA
                     AGKAAAEFVRTTSA"
     gene            1441..2469
                     /gene="tsaD"
                     /locus_tag="VHV99_18285"
     CDS             1441..2469
                     /gene="tsaD"
                     /locus_tag="VHV99_18285"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008353961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX3637394.1"
                     /translation="MLVLGIESSCDETGLALYDTERGLLSHALHSQIAMHREYGGVVP
                     ELASRDHIRRALPLLEQVMAEAGTVPTDIDAIAFTQGPGLAGALLVGASIANALAMAW
                     DLPTVGIHHLEGHLLSPLLVSEPPPFPFVALLVSGGHTQLMRVTDVGEYETLGETLDD
                     AAGEAFDKTAKLLGLGYPGGPEVSRLAEFGTSGAVKLPRPMLHSGDLDFSFSGLKTAV
                     LTHSRKLGMNVCEQSKADLARGFVDAAVDVLTTKSIAALKQTGLNRLVVAGGVGANRQ
                     LREALSAAAKKRRFEVHYPDLSLCTDNGAMIALAGALRLARWPDQAVRDYAFTVKPRW
                     DLTSLAAA"
     gene            complement(2511..>2870)
                     /locus_tag="VHV99_18290"
     CDS             complement(2511..>2870)
                     /locus_tag="VHV99_18290"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004552018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase, FolE2/MptA family"
                     /protein_id="HEX3637395.1"
                     /translation="CPCSKQISQYGAHNQRSHVTIAAEFEGDVEIEDLIRIAEEEASC
                     ELWGLLKRPDEKFVTERAYENPKFVEDLVRDVATRLNADERIVAYVLEAENFESIHNH
                     SAYAVIEHDKRVSALAV"
BASE COUNT          537 a          898 c          890 g          545 t
ORIGIN      
        1 agccacgctg ggaacggctt tgagcgttcc cggcccgctc aggcagaagt tgtccggacg
       61 aactcggcgg cggcttttcc cgccgcgacc cccgacgccc acgcccattg aaaattgtag
      121 ccaccgagcc agccggtgac atcgaccgcc tcgccgatga aatataaacc cgtgacgcgc
      181 gaactcatca tggtagtgga tgaaagctcg cgggtgtcca cgccgccgcg cgtgacctcg
      241 gccttccggt aaccttccgt gccgttcggc gtgagcgtcc agcgcgacag cgattcgccg
      301 atctgacgca gcgttttgtc ggggagatcg gcgaggcggg cgtcagcggc tacgcgctgc
      361 acgtcgagcc aggtttgagc gagccgtgcc ggtacacgct cggcgagaaa cgtgccgact
      421 tgcttgcgcg attcccgctt ggccgcgatc aattcgctgg tggcgtcgac accgggcaac
      481 aagtcgatgc gaatcggctc cgccgggttc cagtagctcg atatctgcag cacgcccggg
      541 cccgataacc cgcggtgcgt gagcagcagg tcctcgctga attccccacg gccgcggcct
      601 gcacccgcgc tgagttccac gggcacggac aatccggata acgccgcgaa cggctcccag
      661 tctgcgggag cgaaggtcaa cgggaccaga gcaggacggg tatcgatgag cttgtggccg
      721 aattgcttcg ccagccggta cgcaaaatcc gtcgcaccaa ttttcgggat cgacaggccg
      781 ccggttgcaa cgacgagtgc cgcgcttgag atcgaaccgg cgttgctgtc gaggaaaaaa
      841 cgggcgccgt cgtgccgaat cgtatcgacg gtgacaggcc ggcgccaggt cacgccgccc
      901 gcgtcgcatt cgcttttcag caattggatg atcgattcgc tggagtcatc gcagaaaagc
      961 tggcctttgt gtttctcatg ccaatccaca cgatgacgct tgagcaaagc gagaaaatcc
     1021 tgcggcgtgt agcgagcaag cgcagagcgg caaaaatgcg gattcgccga gaggaagttg
     1081 gccggtccgg cattgatatt cgtgaagttg cagcggccgc cgcccgagat gcggattttt
     1141 tccgcgagcc gttccgcatg gtcgatcagg accacccggc ggccgttttg cgcggctacc
     1201 gcggcgcaca tcatgcctgc cgcgcccgcg ccgatgactg cgatatcgta ggtttccatg
     1261 gcgcgcattg taacgggtgg tgtgatcagg ttcgaggcga ggttggactg cagggcaagg
     1321 tgtgcgggcc ttgatccgca tcggccgcat aagcaatcgt gcgagcgcaa cgccgctggg
     1381 cggaagagcc caaacccgcc cactgctata cttatctgtt actccaaacg cctctgcccc
     1441 atgcttgttc ttggcatcga aagttcctgc gacgaaaccg gccttgcgct ctacgacacc
     1501 gagcgcggcc tgctttccca cgcgctgcat tcgcaaatcg ccatgcatcg ggaatacggc
     1561 ggcgtagtgc cggaactggc ctcccgcgac catatccgcc gcgcattgcc cctcctcgag
     1621 caagtcatgg cggaagccgg gacggttcca acggatatcg acgctatcgc gtttacacaa
     1681 ggccctggcc tcgccggtgc gctgctggtg ggtgcgagca tcgctaatgc attggctatg
     1741 gcgtgggact tgccgaccgt cgggattcat catctggaag ggcatctgct gtcgccgctg
     1801 ctggtaagcg agccgccgcc gtttccgttc gtcgcgttgc tggtgtcggg tggccataca
     1861 caattgatgc gcgtgaccga cgttggcgag tacgaaacgc tcggcgaaac gctggatgac
     1921 gccgccggcg aagccttcga taaaaccgcc aaactgctgg gcctcggtta tccgggcggc
     1981 ccggaagtct cgcggctcgc cgaattcggc acatcgggcg cagtgaaact gccgcgcccg
     2041 atgctgcatt ccggcgatct ggatttcagc ttcagcggct taaaaaccgc ggtgctcacg
     2101 catagccgca agctcggcat gaacgtctgc gagcaatcga aagcggatct ggcgcggggt
     2161 ttcgtcgatg cagccgtcga cgtgctgacc acaaagtcga tcgctgcgtt aaagcagacg
     2221 ggcctgaacc ggctggtcgt ggcgggcgga gtgggcgcga accggcaatt gcgcgaggcg
     2281 ctgtccgccg ccgcgaaaaa gcgtcgcttc gaagtgcatt acccggattt gtcgctatgt
     2341 accgataacg gcgcgatgat cgccctcgcc ggagcattgc gcctcgcgcg ctggcccgat
     2401 caggccgtgc gcgattatgc gttcacggtg aagccgcggt gggatttgac atcgctggcg
     2461 gccgcgtaag cagcttcatg accgtgatgc gtcatggccg ccacagcgct tcaaacagcc
     2521 agcgctgaaa cccgcttgtc atgttcgatc acggcataag cgctgtgatt gtggatcgac
     2581 tcgaagttct cggcttccag cacataggcc acaattcgtt cgtccgcgtt cagtcgcgtg
     2641 gcgacatcgc ggaccaggtc ttcaacgaat ttcggattct cgtaagcacg ctcagtcacg
     2701 aacttctcgt cgggccgctt aagcaaaccc cacaactcgc acgacgcctc ttcttccgcg
     2761 atccggatca agtcctcgat ttcgacatca ccttcgaact ccgccgcgat agtcacatgc
     2821 gagcgctgat tatgcgcgcc gtactgcgaa atctgcttcg agcacgggca
//
LOCUS       DATASH010000685        10376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5848127,
            whole genome shotgun sequence.
ACCESSION   DATASH010000685 DATASH010000000
VERSION     DATASH010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 10376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10376
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5848127"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1789
                     /locus_tag="VHV99_18295"
     CDS             <1..1789
                     /locus_tag="VHV99_18295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3637396.1"
                     /translation="LRHNAAVRVDRTTSALKSTLERYESLPYLLAEHPIVQDVLVNPS
                     PANIERANLYLEDLNRHARATVTYIIPLDGYCVAASNWHGPGSFIGAGYQFRPYFLDA
                     VKGGVGRFFGIGTISQAPGYYISQPVRREGKIVGVAVVKLDLEWFQGVDASEPLVVAD
                     DHGVIFLSSMPAWKYHTIRPLSGQVADSIYQARQYAQQPIAPLPVTIERTLEDNAQIV
                     RIGGGRYAPRYLASRRGMGEPDWHLITMSPVNPVDADARNATIVTGFGYVSLVLLAFY
                     WRMRRARVREVMRSRSLLQKAYAELNQRVAERTVDLSQANEQLQKEVAERTRAEQELR
                     AAHDELIQASKLAALGQMAAGITHELNQPLAALRGFSDNTRVLLERGDQAAARENLEA
                     IAALTERMGKITNQLKLFVGRARPRSARAPIMRALRNVIALLQKRLQGVEVEFALLDA
                     DTGARTPLKPADDHPELVAHCDDLRLEQVLINLLGNALDATAGMTPPRISVEIEAADT
                     SLSFAVRDNGPGIPDDVLPRLFEPFFTTKEMGQGLGLGLAISSSIARDCGGTLVARNA
                     PDGGALFVLTLRRARVQTSHTSDPLTTGS"
     gene            1804..3156
                     /locus_tag="VHV99_18300"
     CDS             1804..3156
                     /locus_tag="VHV99_18300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX3637397.1"
                     /translation="MLNHGLQVLYIEDDELVRRASVQSLQLAGFDVIGHASAESAAKM
                     ISADFAGVVVSDIRLPGASGLELLAQCHERAPDVPVILVTGHGDISMAVQAMRDGAYD
                     FIEKPFASEHLIETVRRALERRKLVLENLALRRELAGQNTVAPRIIGRSPAIEQVRRL
                     IANIAPTDASVLINGDTGAGKELIARSLHELSPRRDKPFIAVNCGALPEPMFESEMFG
                     YEPGAFTGAAKRRIGKLEHASGGTLFLDEIESMPLALQVKLLRVLQDGVLERLGSNQP
                     IRVNCRVVAAAKGDMAEHVADGSFRRDLLYRLNVVTIALPPLGERREDIVPLFEHFLL
                     DAAVRYQRPAPILTDRQRASLMQREWPGNVRELRNAADRFVLGVAEDPVMSFNDEGVA
                     TQPLKERVEQFERAVIAEALEQAGGVVAAAADRLQLGKATLYEKIKRYGLAAKGEGER
                     "
     gene            complement(3263..3793)
                     /locus_tag="VHV99_18305"
     CDS             complement(3263..3793)
                     /locus_tag="VHV99_18305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX3637398.1"
                     /translation="MSSTPSTTARRANPIRTIIIAVVAVAIAIAGYFAFAGQQRVPDA
                     TFTLLSGQKVSTADLIGKVYLVNFWATSCDTCMKEMPQMVQTYNKFKGKGLEFVAVAM
                     NYDAPMYVANYSQTRQLPFKVAMDDGSAAKQFGNVQLTPTTFLVDKDGKILKRYVGEP
                     QFAELDQLLEKALNAA"
     gene            4165..4683
                     /locus_tag="VHV99_18310"
     CDS             4165..4683
                     /locus_tag="VHV99_18310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637399.1"
                     /translation="MVCALALGGALTACSPTFDWRTIMNNDNGYTVDLPAKPGNDQRA
                     VQIDGKPMQMAMQTAEAGDAVFAVGTVMLPDDDEATQRAALEFLRTGLARNVGAAPDA
                     HAVQIPLAAGGQVPGLEMKLSGEAGSQHETRTVYARLVAHGRHVYQAAIIASKPLQQE
                     QVDQFFSSFQLY"
     gene            complement(5016..6644)
                     /locus_tag="VHV99_18315"
     CDS             complement(5016..6644)
                     /locus_tag="VHV99_18315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEX3637400.1"
                     /translation="MSLAVVRSRAPASGRAPEVTVEVHLANGLPSFSIVGLPDLEVRE
                     SRERVRAALQNCSFDFPVRRITVNLAPADLPKESGRFDLPIALGILAASGQIPAESLL
                     NREFAGELSLTGALRPMRGAFAMACGTARSGASHGTLSGVPSGASTFGASAFISSGEQ
                     EAPEADSQCTPQLYLPAASAAEAALVPGVDVYGATDLPSLCAHLAGAPDARLYPVAAP
                     ELSETAPAAVPDMADVIGQRGARRALEVAAAGGHHVLLIGPPGAGKSMLAARLPGLLP
                     PMTDDEALSSAALLSASRAGFTPSQWRQRPFRAPHHSSSAPALVGGRNPPQPGEITLA
                     HLGVLFLDELPEFERHVLETLREPLEAGRITISRAALQADFPAACQLIAAMNPCPCGW
                     RGDPNGRCRCTPEIAARYLRKLSGPLLDRIDIQIEIPALTPAELSARSSVAGESSASI
                     ALRVNAARERQLARQGKTNRELDGREVDEVCRPDAAGEALLRQAGERFGWSARAYYRV
                     LKVARSIADLAGTAMPSAAQIGEAIQYRRAFGSP"
     gene            complement(6766..7023)
                     /locus_tag="VHV99_18320"
     CDS             complement(6766..7023)
                     /locus_tag="VHV99_18320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEX3637401.1"
                     /translation="MKQPNDVFNDFQARMSELFKNSPAKDVERNVKAMLSQGFSRLDL
                     VTREEFDTQTQVLVRTRARLEELERRVAELEQKLPVTTQTP"
     gene            7410..7748
                     /locus_tag="VHV99_18325"
     CDS             7410..7748
                     /locus_tag="VHV99_18325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX3637402.1"
                     /translation="MKLITAIIKPFKLDEAREALSAIGVSGITVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIEAAVSDDIVDQAIEALERAARTGKIGDGKIFVTPIEQVIRI
                     RTGETGADAL"
     gene            7792..9309
                     /locus_tag="VHV99_18330"
     CDS             7792..9309
                     /locus_tag="VHV99_18330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015001510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX3637403.1"
                     /translation="MRKLLMSMLMAGSLLAGGIGAALADDASAPAAASAAASDTTASA
                     PAPDASAAPAAAAASAPAADASAAPAASAAAAPDASAASAAAAPAAPTAPFSVDSSKI
                     NSGDTAWMLTSTALVLFMTIPGLALFYGGMVRKKSVLAILMQSFAITCLVSIVWVVVG
                     YTLAFTPGNSFIGGFSRFFMAGMNYIKGDKATTLTVSHLAPTIPETVYVVYQMTFAII
                     TPALITGAFADRMKFSAMLVFMTLWSIIVYSPIAHMVWEPTGWLASAGILDFAGGTVV
                     HINAGIAALVCALVLGKRVGYGKEAMAPHNLTLTLIGGAMLWVGWFGFNAGSAVAADG
                     RAGFAMFATQVATAAAALAWMFAEWATKGKPSVLGIVSGAVAGLVAITPASGFVGMAG
                     SLVIGIAAGVICFWSATWLKHKLGYDDSLDAFGVHCIGGIVGALLTGVFAVKDIGGAD
                     GSVILQAKGVLTTLVYSGVMSYILLKIIDMVMGLRVTEEEEREGLDVTLHGEHVE"
     gene            9543..>10376
                     /gene="gshA"
                     /locus_tag="VHV99_18335"
     CDS             9543..>10376
                     /gene="gshA"
                     /locus_tag="VHV99_18335"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013337902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEX3637404.1"
                     /translation="MVPHLVTALNGPLLDLERKILDATPAIERWFRLEWQEHTPPFYC
                     SVDLRNAGFKLAPVDTNLFPGAFNNLPQEVLPLAVQAAMASIEKICPDAKNLLVIPER
                     HTRNAFYLENVARLAAIMRQAGLNVRFGTLDESIVGPVTIALSDGQKLVLEPLERSQR
                     RLGLKNFDPCSILLNNDLSGGIPPVLENLHEQYLLPPLHAGWAVRRKSTHFSCYDDVA
                     KKFAKMVEIDPWMINPYFAHVEGVDFQERTGEEALTDAIDGVLKKIAKKYREYGISEK
                     PY"
BASE COUNT         1880 a         3178 c         3359 g         1959 t
ORIGIN      
        1 tttgcggcac aacgccgcgg tgcgggtcga tcgcactacg agcgcgctga aaagcacgct
       61 cgagcgctac gagtcgctgc cttatctgct ggccgaacat cccattgtgc aagacgtgct
      121 ggtcaatccc agtccagcca acatcgaacg cgccaatctc tatctcgaag acctcaaccg
      181 ccacgcgcgg gccacggtca cctacatcat tccgctcgac ggctattgcg tcgccgcgag
      241 caactggcac gggccgggca gcttcatcgg cgcgggctat caattccggc cgtattttct
      301 cgatgcggtg aagggcggcg tgggccgctt cttcggtatc ggcacgattt cgcaggcgcc
      361 gggttactac atctcgcagc cggtgcggcg cgagggcaag atcgtcggcg tggcggtcgt
      421 caaactcgat ctcgaatggt tccagggcgt ggacgcgtcc gagcctctgg ttgtcgccga
      481 tgaccatggt gtgatctttc tgtcgtcgat gccggcgtgg aaataccaca cgatccggcc
      541 gttgtcgggg caggtggccg attcgatcta tcaggcgcgc caatatgcgc agcaaccgat
      601 cgcgccgctg ccggtgacga tcgagcgcac gctcgaagac aacgcgcaga tcgtgcgcat
      661 cggcggtggc cgctatgccc cgcgctatct cgcgtcgcgg cgcggcatgg gcgaaccgga
      721 ctggcatctg attacgatgt cacccgtcaa tccggtcgat gccgacgcgc gcaatgcaac
      781 catcgtcacc ggctttggat acgtgtcgct ggtgttgctc gccttctact ggagaatgcg
      841 ccgcgcccgg gtgcgcgagg tgatgcgcag ccgctccctg ctgcagaagg cctacgctga
      901 attgaaccag cgcgtggccg agcgcaccgt ggatctctcg caggcaaacg agcaattgca
      961 gaaggaagtc gccgagcgga cgcgcgccga acaggagctg cgcgcggccc acgacgaact
     1021 gatccaggcc agcaagctcg ccgcgctggg tcagatggcg gcgggcatca cgcacgaatt
     1081 gaatcagccg ctcgcggcgc tgcgcggttt ctcggacaac acgcgcgtgc tgcttgaacg
     1141 cggcgaccag gcggctgcgc gcgaaaatct tgaagcgatc gcggcgctca ccgagcgcat
     1201 gggcaagatc accaatcagt tgaagctgtt cgtcggccgc gcgcggccgc gcagtgcgcg
     1261 tgcgccgatc atgcgggcgt tgcgcaacgt gatcgcgttg ctgcaaaagc gtttgcaagg
     1321 cgtcgaagtc gagttcgctt tgctcgatgc cgacaccggc gcgcgtacgc cgctgaagcc
     1381 cgcggacgat catcccgagc tggttgccca ttgcgacgat ctacggctcg aacaggtgct
     1441 gatcaatctg ctcggcaacg cgctcgacgc aacggcgggg atgacgccgc cgcgcatttc
     1501 ggttgagatt gaagcggctg acacgagcct gtcgttcgcc gtgcgcgaca acggcccagg
     1561 cattcccgac gacgtgctac cccgtctgtt cgaaccgttc ttcacgacga aggaaatggg
     1621 ccaggggctg gggctcggtc tcgcgatttc atcgtcgatt gcacgcgatt gcggcggcac
     1681 cctggtggcg cgcaacgcac cggacggcgg tgcattattc gtgttgacgc tgcggcgcgc
     1741 gcgcgtgcag acgagccata catcggaccc gctcaccacg ggttcctgac agatgagacc
     1801 gacatgctga accacggctt gcaagtgctg tacatcgaag acgatgaact ggtgcggcgc
     1861 gccagcgtgc agagtttgca gctggcgggc ttcgacgtga tcggccatgc ctcggcggaa
     1921 tcggcggcaa agatgatcag cgcggatttc gccggcgtgg tggtcagcga tattcgattg
     1981 cccggcgcga gcggtctcga actgctcgcg cagtgccatg agcgcgcgcc cgacgtgccg
     2041 gtgattctgg tcaccggtca tggcgatatt tcgatggccg tgcaggcaat gcgtgacggc
     2101 gcgtatgact tcatcgaaaa gccgtttgca tccgagcatc tgatcgaaac cgtgcgacgt
     2161 gcgctggagc gccgcaagct ggtgctggag aacctcgcgc tgcgccgtga gctggccggg
     2221 cagaacacgg tggcgccgcg catcatcggc cgcagtccgg cgatcgagca ggtgcggcgg
     2281 ctgatcgcga atatcgcgcc gaccgacgcg tcggtgctga tcaacggcga caccggcgcg
     2341 ggcaaggagt tgatcgcgcg cagtctgcat gaactgtcgc cgcgacgtga caagccgttt
     2401 attgcggtga attgtggcgc gcttccagag ccgatgttcg agtcggaaat gttcggctac
     2461 gaacccggcg cattcaccgg cgcggcaaaa cgccggatcg gcaagctcga acatgcttcg
     2521 ggcggcacgc tgtttctcga cgaaatcgag agcatgccgc tcgcgttgca ggtcaagctg
     2581 ctgcgcgtgt tgcaggacgg cgtgctggaa cggctcggct cgaatcagcc gatccgcgtg
     2641 aattgccgcg tggtggccgc cgcgaagggc gacatggccg agcatgtcgc ggacggctcg
     2701 ttccgtcgcg acttgctgta ccggttgaac gtggtgacga tcgcgctgcc gcccttgggc
     2761 gagcgccgcg aggacatcgt gccgttgttc gaacacttcc tgctggacgc ggcggtgcgc
     2821 taccaacgcc ctgcgccgat tctcacggac cggcagcgtg cgagcctgat gcagcgcgaa
     2881 tggccgggga atgtgcgcga actgcgcaat gccgccgacc gcttcgtgct cggggtcgcc
     2941 gaggatccgg tcatgtcttt taacgacgag ggcgtcgcga cgcaaccgtt gaaggaacgt
     3001 gtcgaacagt ttgagcgggc ggtgatcgcg gaggcgttgg agcaggccgg cggtgtggtc
     3061 gcagctgcgg cagacaggct gcagctaggc aaggcgacgc tctacgagaa gatcaagcga
     3121 tatggcctcg cggccaaagg ggaaggggag cggtgaggat ttgaagcgcc ggcggcgagg
     3181 aggtatgacc ccggcgccgg cgcgtttgaa tcagggtgct tttcaggctt ttcgtggcaa
     3241 tgaggcggca accaggtggc aatcaggccg cgttcagcgc tttttcgagc aactggtcga
     3301 gttccgcgaa ttgcggttcg ccgacgtagc gcttcagaat cttgccgtcc ttgtcgacca
     3361 ggaacgtagt cggcgtgagc tggacgttgc cgaactgctt ggcggccgag ccgtcgtcca
     3421 tcgccacctt gaatggcaac tggcgggttt gcgagtagtt ggccacgtac atcggcgcat
     3481 cgtagttcat cgcgacggcg acaaattcga gccctttgcc cttgaatttg ttataggtct
     3541 gcaccatttg cggcatttct ttcatacagg tgtcgcagct ggtcgcccag aaattgacca
     3601 gataaacctt gcctatcagg tcggcggtcg aaactttctg gcccgacagc aaagtgaacg
     3661 ttgcatcggg cacgcgctgt tggcccgcga acgcgaaata gccagcaatc gcaatcgcga
     3721 cggccaccac ggcgatgatg atagtgcgaa tcggattcgc gcgccgagcg gtggtggacg
     3781 gagtggaact catgagcgga acctcgagag tcgcgcggaa cgcgcgggac agtgcgttgg
     3841 tctggattgc gtttggactg ttagatccgg gtgctgcata gcgtcgctat tgtagcccca
     3901 ttccggccat gcggcagagc gcaggccggg aggggataat agggctctac gttcctgtca
     3961 gtccgatctt ccgtgtccat gctccgaact gcgtttcgct tcctcgcccg aattcccgta
     4021 gccttgcgaa tcacgcgaat tcccataacc ccgtttggcc gctccgttag ccgttcctcg
     4081 cgtttctccc tggcccacag tcgcgcggcc agcgctgctt ctcgcttgcc tcgctcgcag
     4141 cgggcgcgtg ccgcacttgg cacgatggtt tgcgcgctgg cgttgggcgg cgcgttgaca
     4201 gcctgttcgc cgaccttcga ctggcggacc atcatgaaca acgacaacgg ctacacggtc
     4261 gacctgccgg ccaagccggg caatgaccag cgcgcggtcc agatcgacgg aaagccgatg
     4321 cagatggcga tgcagacagc cgaagccggc gacgccgtgt tcgcagtcgg caccgtcatg
     4381 ctgcctgatg acgacgaagc gacccagcgc gccgccctcg aattcttgcg cacgggtctc
     4441 gcccggaacg tcggcgccgc gccagacgcg cacgcggtgc aaatcccact cgccgccggc
     4501 ggtcaggtac ccggtctgga aatgaagctg agcggtgagg ccggttccca acacgaaacg
     4561 cgcaccgtct atgcacggct ggtcgcgcac ggccgacatg tttatcaggc cgcgatcatt
     4621 gcttccaagc cgttgcagca ggagcaggtc gaccaatttt tttcgtcgtt ccaactgtat
     4681 taagagcggt agttttggct gattcggcgc gcggtagatc ggagggcata gtgaaagttc
     4741 cttcgtagca tcgtgcgcta attcccggtt tacgttgagg ttttttggtt actcacagct
     4801 cctgtggata actctgttga gaactccgcg ttttttgccg cggaggccca tccggtgggc
     4861 attctgtcag agtgaggtca cttaggacgc gcaaaaaaac ccaattaaat caatgtcatt
     4921 tggccgcatg cttccgtacg cctttcagat gtttcaaatc ttatctggaa cgcgcacatg
     4981 tgcataagtc aagacttgac agtagatttt tcgccttatg gcgacccaaa agcccggcga
     5041 tattgaatgg cttcaccaat ctgcgcggcg ctcggcatgg cggtgccggc gaggtcggcg
     5101 atgctccgcg ccaccttcag gactctgtag taagcacgcg ccgaccagcc gaagcgctcg
     5161 ccggcctgac gcagcaacgc ttcgccggct gcgtccgggc ggcagacctc gtccacttcc
     5221 cgcccgtcca gttcccgatt ggttttacct tgccgcgcta gttgccgctc gcgagcggca
     5281 tttacccgca gtgcgatcga cgcgctcgat tcaccggcaa cgctggagcg tgccgacaac
     5341 tccgccggcg taagcgcggg aatctcgatc tgaatgtcga tccggtcgag cagcggcccc
     5401 gagagtttgc gcagatagcg cgccgcgatc tccggcgtgc agcggcagcg gccgttggga
     5461 tcgccgcgcc atccgcatgg acaagggttc atcgcggcga tcagttggca ggctgccgga
     5521 aagtccgctt gcagggcggc ccgcgagatc gtgatgcgac cggcctcgag cggctcgcgc
     5581 agggtttcca gcacatgacg ttcgaactcc ggcagctcgt ccagaaacag cacacccaga
     5641 tgcgccaagg tgatttcgcc gggttgcggt ggattgcgcc cgccgaccag cgccggtgcg
     5701 ctcgacgaat gatgcggcgc gcggaatggc cgttgccgcc attgcgacgg cgtaaagccg
     5761 gcccggctgg cggacagcaa ggcggccgag ctgagcgctt cgtcgtcggt catgggtggg
     5821 aggaggccag gcaggcgggc ggcgagcatt gatttgcccg cgccaggcgg cccgatcagc
     5881 aagacgtggt gtccgccggc cgccgcgact tccagcgcgc ggcgcgcgcc gcgctggccg
     5941 atcacgtcgg ccatgtcggg aacagcggcg ggtgcggtct cgctgagctc gggggccgcg
     6001 acagggtaga gccgggcatc gggcgctccg gccagatgcg cgcacagtga cggcagatcg
     6061 gtggcgccat agacatcgac gcccggcacg agcgccgcct cggccgcgct ggcagcaggc
     6121 aaatagagct gcggggtaca ctggctatcc gcctcgggtg cctcctgctc gccggacgaa
     6181 ataaaagctg aagcaccaaa cgttgaagca ccactgggga cgccgctcag cgtgccgtga
     6241 gaagcgccgc tgcgggccgt cccgcaagcc atcgcgaaag ccccgcgcat tggccgcaac
     6301 gcaccggtca gtgatagttc gcccgcgaat tcccgattca ggagggactc ggccggaatc
     6361 tgcccgctcg ccgcgagaat gcccaaagcg atcggcagat cgaatcggcc ggattctttc
     6421 ggcaaatcgg cgggagcgag attgacggtg atgcgccgca cagggaaatc gaagctgcag
     6481 ttctgcagcg ccgcgcgcac gcgctcgcgg ctttcgcgca cttccaggtc gggaaggccg
     6541 acgatcgaaa aagagggtaa tccgttggcg aggtgaacct cgacagttac ttcgggcgcg
     6601 cggccagagg ccggcgcgcg actgcgcacc acagcaagcg acatgttttc tcccgtcgga
     6661 cggcgcgcct tgggcgcgcg cgagtcccct aaaacgctga tgacgtcacg gctcgcttgc
     6721 cagtgatgtg cagcactgcg cagctaaagc gctgcgagca aagcttcagg gcgtttgcgt
     6781 ggtgaccggc agcttctgtt cgagctcggc aacgcggcgt tccagttctt cgaggcgcgc
     6841 acgggtgcgc accagcacct gggtctgcgt atcgaattcc tcacgcgtga ccaggtcgag
     6901 cctggaaaag ccctgcgaca gcatggcttt cacgttgcgt tcgacgtctt tggccggcga
     6961 gttcttgaac agctcgctca tgcgggcctg aaaatcgtta aagacatcgt tcggttgttt
     7021 catggtgttc cccttgatgc acgaaaaatg tgcatgtttc gttacgcctg tgctttgcca
     7081 cggttgatgg gccgttttgg tgcatgaccg attgtccgcc gctgccgcat gagcccgttt
     7141 ggtgcgcgct caaaacgtca taagccttcg aacactctag caatgatcca tacgccgcgc
     7201 caccgtcctc cgccaacgct ggccgtccgg ccagggcggc ttgcgcccct tttgtagcgt
     7261 ggcgggagcg aagtctgaca caacatggca tgcgagttgc tgttactgcc ccgcgcgcac
     7321 tgccggatgg tttttcctgg cagcggaacg agaccacgcg aacgggtcca ggtacgcaaa
     7381 cagggttacg caactaagcg agggatttca tgaaactcat taccgcaatc atcaagccgt
     7441 tcaagctcga tgaggcgcgc gaagccttgt cggccatcgg cgtctccggc atcacggtga
     7501 ccgaagtcaa aggcttcggt cgtcagaagg gccatacgga actgtatcgg ggcgccgaat
     7561 acgtcgtcga tttcctgccg aaggtgaaga tcgaggcagc ggtctcggac gacatcgtcg
     7621 atcaggcgat cgaggcgctc gaacgcgcgg cacgcaccgg caagatcggc gacggcaaga
     7681 tttttgtcac gccgatcgag caagtgattc ggattcgcac cggggagacc ggcgcggacg
     7741 ctctgtaata ccaaaagata gcgacacaga tagcgacaag aggaaacgaa gatgcgcaaa
     7801 ttattgatgt ccatgctgat ggccggttcg ctgctcgcgg gcggtatcgg cgccgcactc
     7861 gcggacgacg cttccgcccc cgcggccgcc tcggccgctg cttccgatac gacggcgagc
     7921 gcgccggcgc cggacgcctc ggcagcacct gctgccgcag cggcctcggc accggccgct
     7981 gacgcgtccg ccgcaccggc tgccagcgcc gcagccgcac ctgatgcatc ggccgcatcc
     8041 gctgctgccg caccggcagc tccgaccgcg ccgttctcgg tcgattcgtc gaagatcaat
     8101 tcgggcgaca cggcctggat gctgacctcc accgcgctcg tgctgttcat gacgatcccc
     8161 ggtctggcac tgttctacgg cggcatggtc cgcaagaaga gcgtgctcgc gatcctgatg
     8221 caaagcttcg cgatcacctg cctcgtgtcg atcgtctggg tggtggtggg ctacaccctg
     8281 gccttcacgc cgggcaactc gttcatcggc ggcttctcgc gcttcttcat ggcgggcatg
     8341 aactacatca agggcgacaa ggccacgacg ttgaccgtca gccacctcgc accgacgatc
     8401 ccggaaacgg tctacgtcgt ctaccagatg acgttcgcga tcattacccc ggccttgatc
     8461 acaggcgcat tcgccgatcg tatgaagttc tcggcgatgc tggtgttcat gacgctgtgg
     8521 tcgatcatcg tctactcgcc gatcgcgcac atggtttggg aaccgaccgg ctggctggct
     8581 agcgcgggca tcctcgactt cgcgggcggc acggtggtgc acatcaacgc cggtatcgcg
     8641 gcgctggtct gcgcgctggt gctcggcaag cgtgtcggct acggcaagga agcgatggcg
     8701 ccgcacaacc tgacgctcac gctgatcggt ggcgccatgc tgtgggtagg ctggttcggc
     8761 ttcaacgcgg gttcggcagt ggcggcagac ggccgtgccg gtttcgcgat gttcgcaacg
     8821 caagtggcaa ccgcagcagc agcactcgca tggatgttcg ctgaatgggc aaccaagggt
     8881 aagccgtcgg tactgggtat cgtgtccggc gccgtggcag gtctggtggc gattacgccg
     8941 gcttcgggct tcgtcggtat ggcaggttcg ctggtgatcg gcatcgcggc aggcgtgatc
     9001 tgcttctggt cggcaacgtg gctcaagcac aagctcggtt acgacgactc gctcgacgcg
     9061 ttcggcgtgc actgcatagg cggtatcgtg ggtgcgctcc tgaccggcgt gttcgcggtc
     9121 aaggacatcg gcggcgcgga cggcagcgtc atcctgcaag ccaagggcgt gctgaccaca
     9181 ctggtctaca gcggcgtgat gagctacatc ctgctgaaga tcatcgacat ggtgatgggt
     9241 ctgcgcgtga cggaagaaga ggagcgcgaa ggcctggacg tgaccctgca cggcgaacac
     9301 gtcgaataag gattcccccg caagttccag ggcttgcgga tcggaatcaa caaccgcagc
     9361 aactttggcc cgccttttac ggcgggcttt tttcttgcgg cttatcttgt taacagtaga
     9421 tttccccgcc tatcatggcg atagcgagaa tgcattcgcg tccgcttgcg aatggagaaa
     9481 ccgtccccaa atgggaaaag catttgctct acaatctttt ttctccagtc tgcgagtgat
     9541 caatggttcc gcacctagtt acggcgttaa acggcccgct gctcgacctc gagcggaaga
     9601 tcctcgacgc tacccccgcc atcgaacgct ggttccggct cgaatggcag gagcacacgc
     9661 cgccgttcta ctgttcggtc gacttgcgta acgcgggctt caagctcgcg ccggtcgaca
     9721 ccaatctgtt tcccggcgca ttcaataatc tgccgcagga agtgttgccg ctcgccgtgc
     9781 aggccgcgat ggcgtcgatc gagaagatct gcccggacgc gaagaatctg ctggtgattc
     9841 cggagcgcca tacgcgcaac gccttttatc tcgagaatgt cgcccgtctg gccgcgatca
     9901 tgcgccaggc cggcttgaac gtgcggttcg gcacgctcga cgaaagcatc gtcgggccgg
     9961 ttacgatcgc cttgtccgac ggccagaagc tggtgctcga accgctcgag cgttcgcaac
    10021 ggcgcctcgg tctgaaaaac ttcgacccgt gctcgattct gctgaacaat gatctgtccg
    10081 gtggcattcc gcccgtgctg gaaaacctgc acgagcagta tttgctgccg ccgctgcatg
    10141 ccggctgggc cgtgcgccgc aaatccacgc atttctcctg ttatgacgac gtcgcgaaga
    10201 agttcgcgaa gatggtcgaa atcgatccgt ggatgatcaa tccgtacttc gcgcatgttg
    10261 aaggcgtcga tttccaggag cgcacgggtg aagaagcgct gacggatgcg atcgacggcg
    10321 tgctcaagaa gatcgccaag aagtatcgcg agtacggcat ctccgagaaa ccgtat
//
LOCUS       DATASH010000686         2355 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_903246,
            whole genome shotgun sequence.
ACCESSION   DATASH010000686 DATASH010000000
VERSION     DATASH010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903246"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..1072)
                     /gene="miaA"
                     /locus_tag="VHV99_18340"
     CDS             complement(125..1072)
                     /gene="miaA"
                     /locus_tag="VHV99_18340"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEX3637405.1"
                     /translation="MTSHTPTPIPCLLGPTASGKTAAALALAARRPVEIISVDSALVY
                     REMDIGTAKPTPEERAVAPHHLIDIVDPADAYSAAEFRSDALRLIGEISTRGRLPLLV
                     GGTMLYYKALTQGLNDLPAANADVRAALDADAARDGWPALHARLAAVDPVTAARLAPN
                     DSQRIQRALEVFMLSGQAMSALLAAPARVDRAAQAWRFVPIALEPSERSVLHARIEKR
                     FDAMLAEGFIDEVVKLRERGDLLPEMPSMRCVGYRQAWEYLDGAVDYSTMRDKGVFAT
                     RQLCKRQLTWLRGMTELVVVDCCDPGATAQVVGLVEELI"
     gene            complement(1116..>2355)
                     /gene="mutL"
                     /locus_tag="VHV99_18345"
     CDS             complement(1116..>2355)
                     /gene="mutL"
                     /locus_tag="VHV99_18345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEX3637406.1"
                     /translation="DQQYFFVNGRFVRDKLLTHAVRAAYEDVLHGDRYPSYVLFLDLP
                     PEAVDVNVHPSKIEVRFRDSRSIHQFVFHAVQRALARHAGASPETTAGGHAAHLQASA
                     GGPASFGATPLGGVGGTAGSFGTGSNAVAGAGGIGGGFGSSQQPGNTWMRQARMTQGT
                     LPVAQPLAFYDALFGRKDTNAGTAEGATLFEARDSAAEAPAPYNTSTPYASPPFNAAD
                     EQPLGFALGQIHGIYVLAQNAHGLVIVDMHAAHERILYEQFKNALADRTIAVQPLLIP
                     QTMQADPIEIGTVEEERDTLDALGFDLAVLSPTTLAIRAVPALLKDADLQALARAVLS
                     DLHAFGGSRVLTERQHELLGTLACHHAVRANRRLTLDEMNALLRQMEATERADQCNHG
                     RPTWYQLTLSDLDRLFMRGQ"
BASE COUNT          437 a          766 c          756 g          396 t
ORIGIN      
        1 aacaaccagc ttgaacagtc agcttaaaca accagcttga acagtcagct taaacaacca
       61 gcttgaactt cggctggttt gcctgcgcgg tgtacagaaa gaacgcgccg cgcgggcgaa
      121 cacttcaaat caattcttcg actaagccga caacctgcgc agtcgcgccg ggatcgcagc
      181 agtccaccac aaccagctct gtcatgccgc gtagccacgt gagctgccgc ttgcacaatt
      241 gccgggtggc aaagaccccc ttgtcacgca tcgtcgaata gtcgaccgct ccgtcgagat
      301 attcccaggc ctgccggtat cccacgcagc gcatggatgg catctcgggc aataaatccc
      361 cgcgttctcg caatttcacc acttcgtcga tgaagccttc agccagcatg gcatcgaagc
      421 gtttttcgat gcgtgcatgc agcacgctcc gctcggatgg ctcaagcgca atcggcacga
      481 agcgccaggc ttgcgcagca cgatcgacgc gagcgggcgc ggccagcaaa gcggacatcg
      541 cttgcccgct cagcatgaaa acctcgagcg cccgctgaat ccgctgcgaa tcgttcggcg
      601 cgaggcgcgc ggccgtgacc ggatcgacag cggcaagccg cgcatgcagc gccggccagc
      661 cgtcgcgtgc ggcgtcggca tcgagcgctg cacgcacgtc ggcattggcg gcgggcaggt
      721 cgttcagtcc ttgcgtgagg gccttgtagt acagcatcgt cccgccgacc aataacggca
      781 gccgcccgcg cgtgctgatc tcaccgatca ggcgtaacgc gtcgctacga aattccgccg
      841 ccgaatacgc atccgccgga tcgacgatat cgatcagatg gtgcggcgcc accgcgcgct
      901 cttcaggcgt cggcttggcc gtgccgatat ccatctcgcg atataccagc gccgaatcga
      961 cgctgatgat ttcgaccggc cgccgtgccg ccagcgccag cgcggcagcg gtcttgccgg
     1021 atgcggtcgg gcccagcagg caagggatcg gcgtgggagt gtgcgaggtc ataggcaaca
     1081 cggggcgcac cggaccagcg taagcaggcg caccgtcatt gcccacgcat gaacagccga
     1141 tcgagatcgg acagcgtcaa ctggtaccac gttggacggc cgtggttgca ctggtcggcg
     1201 cgctcggtcg cctccatctg gcgcagcagc gcgttcatct cgtcgagtgt gagacgccgg
     1261 ttcgcgcgca ccgcgtgatg gcacgcgagc gtgccgagca gttcgtgctg acgctcggtc
     1321 aacacgcgtg aaccgccgaa cgcatgcaga tcggagagaa ccgcgcgggc cagcgcctgc
     1381 agatcggcgt ctttcagcaa cgcaggcacg gcgcggatcg cgagcgtggt gggcgacagc
     1441 acggcgaggt cgaaaccgag tgcatccagc gtgtcgcgct cttcttcgac cgtgccgatt
     1501 tcgatcggat cggcctgcat ggtctgcggg atcaagagcg gctgcacggc aatcgtgcga
     1561 tcggccagtg cgttcttgaa ctgctcgtac aggatgcgct cgtgcgcggc atgcatatcg
     1621 acaatcacga gtccgtgcgc gttctgcgcg agcacataga tgccgtgaat ctggccgagg
     1681 gcgaagccga gcggctgctc gtcggccgca ttgaaagggg gcgatgcgta gggtgtggac
     1741 gtgttgtatg gcgcgggtgc ctcggcggcc gagtcgcgcg cttcgaagag cgtcgcgcct
     1801 tccgctgttc ctgcgttggt gtctttgcgg ccgaagagag cgtcgtagaa cgccagcggt
     1861 tgggccaccg gcagcgtgcc ttgtgtcatg cgcgcctggc gcatccacgt attgccgggc
     1921 tgctgcgagg atccaaatcc gccaccgatc ccgccagccc ccgcgactgc attcgagccc
     1981 gtgccgaagc tgccggccgt accgcctaca ccgcccagcg gcgtagcgcc gaacgaagcc
     2041 ggcccgcctg ccgacgcttg cagatgcgct gcatgcccac cagcggtcgt ttccggcgac
     2101 gcgcccgcgt gccgcgccag cgcgcgctgc acggcgtgaa acacgaactg gtgaatcgaa
     2161 cgtgaatcgc gaaaccgcac ttcgattttc gacggatgca cgttcacgtc gacggcttct
     2221 ggcggcagat cgagaaacag cacgtaggac ggatagcggt cgccgtgcag cacgtcttcg
     2281 taagcggcgc gcaccgcgtg cgtgagcagc ttgtcgcgca cgaaccggcc attgacgaag
     2341 aaatactgct gatcg
//
LOCUS       DATASH010000687         4369 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6439597,
            whole genome shotgun sequence.
ACCESSION   DATASH010000687 DATASH010000000
VERSION     DATASH010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4369
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6439597"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..2106)
                     /locus_tag="VHV99_18350"
     CDS             complement(181..2106)
                     /locus_tag="VHV99_18350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3637407.1"
                     /translation="MSLYTITGAQLAFGHVALLDHADFSLEAGERVGLIGRNGAGKSS
                     LLKIVADLSKPDDGLVTRQQGLTSVYVPQEPEFDTDDTVFDAVAAGLTHARALLDEYD
                     VVANQLADEPEGPAHDELMSRMNTLQSALDHSDAWNWSTRVATTLQQIGLNGEARVGS
                     LSGGMQKRVALARALVVQPDVLLLDEPTNHLDFDGIRWLEDLLVSLRACLLFITHDRA
                     FLDRVATRIVELDRGRLLSYPGNFSAYQTRKAQQLEVEQVEAAKFDKLLAQEEVWIRK
                     GVEARRTRSVGRIARLVQMRNERAERRNVQGNVKLDVGQGEKSGKIVAELTDVTKRYG
                     SRTVVGTFTATVMRGDKIGFVGPNGAGKTTLLKMILGELPPDEGTVRIGTNLQVAYFD
                     QMRAQLDLDKSLADTISPGSEWVEVNGQKKHVMSYLGDFLFAPERARSPVKSLSGGER
                     NRLLLARLFARPANVLVLDEPTNDLDIPTLELLEELLTEYDGTVLLVSHDRAFLDNVA
                     TSVIASEGGGKWREYVGGFTDWQIQRDRSQQMAQDAQKDAAKEVAASKDTGAARNTQR
                     AVKLSFKDQRELEALPAKIAALEAEQKTIGAQLEDGSVFAKDAQEGTRLTERYAAIEE
                     ELLIALERWDELENKRK"
     gene            complement(2354..2755)
                     /locus_tag="VHV99_18355"
     CDS             complement(2354..2755)
                     /locus_tag="VHV99_18355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4399 domain-containing protein"
                     /protein_id="HEX3637408.1"
                     /translation="MLRNKWLAGTVLAGLLAVSGAAQAAGVSFVQPADGATVSNPVHV
                     VFGVDGMKIAPAGTMTEGTGHHHLLIDGKPLPKGEVIPANDKSLHFGKGQTETDLTLP
                     PGDHTLTLQFGDGAHRSYGPEMSKTITVHVK"
     gene            3081..3272
                     /locus_tag="VHV99_18360"
     CDS             3081..3272
                     /locus_tag="VHV99_18360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEX3637409.1"
                     /translation="MSEVIEVTEYKSWVCLICGWIYNEEEGIPEDGIAPGTRFAAIPD
                     DWRCPLCDVSKAEFAVVEF"
     gene            3505..3579
                     /locus_tag="VHV99_18365"
     tRNA            3505..3579
                     /locus_tag="VHV99_18365"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3538..3540,aa:Arg,seq:cct)
     gene            complement(3751..4083)
                     /locus_tag="VHV99_18370"
     CDS             complement(3751..4083)
                     /locus_tag="VHV99_18370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637410.1"
                     /translation="MAGWLRHSFPRVSRYGEIGVDVFFVIFGFVMAWVTHGVPRGLAS
                     ARSFIATRIARVSPLYRISRRFSLRRDDGWEAVRLLTMRRIRQLTVLNNALPGATVCL
                     SDNKPGAA"
     gene            complement(4167..>4369)
                     /locus_tag="VHV99_18375"
     CDS             complement(4167..>4369)
                     /locus_tag="VHV99_18375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018431611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HEX3637411.1"
                     /translation="GRDWILGDAYSIADIATFPWVRNLIGFYEAGDLVGIDDFPNVKR
                     VLVAFVARPAVAKGLEIPKRPA"
BASE COUNT          815 a         1368 c         1339 g          847 t
ORIGIN      
        1 aattcacggc atgcgggatt ggccgtttgg ataaggggca gagtacggct tcggccaatt
       61 atgcgaccgc gacgagaccg ctggaggagc gtgaaaacat gacgacgggg ccgctgcgcg
      121 gccacgcccc cgcgctctgc aggaaagcgg cgcaaatccg gatcgcaccg ccctcgtgcc
      181 tcacttgcgc ttgttttcca gctcgtccca gcgttccagc gcgatcagca attcctcttc
      241 gatcgcggcg taacgctcgg tcagacgcgt gccttcctgt gcgtctttcg cgaagaccga
      301 accgtcttcg agttgagcgc cgatggtttt ctgctcggct tcgagcgcgg caattttcgc
      361 cggcaacgct tccagctcgc gctgatcctt gaaggacagc ttgacggcgc gctgcgtatt
      421 gcgggccgcg ccggtatcct tcgacgccgc cacttccttg gccgcgtctt tctgtgcgtc
      481 ctgcgccatc tgctgcgaac ggtcgcgttg aatctgccag tcggtaaagc cgccgacata
      541 ctcgcgccac ttgccgccgc cttcagaagc gatcaccgaa gtcgcgacgt tgtcgagaaa
      601 cgcgcgatcg tggctgacca gcagcacggt gccgtcgtat tcggtgagca gttcttcgag
      661 caattcgagc gttggaatgt cgaggtcgtt ggttggttcg tcgagcacca gcacgttggc
      721 cggccgggcg aacagacgcg ccagcaacag gcggttccgc tcgccgcccg agagcgactt
      781 gaccggcgaa cgcgcgcgtt ccggcgcgaa caggaagtcg ccgagatagc tcatcacgtg
      841 cttcttctgg ccgttgactt cgacccattc gctccctggg ctgatcgtat ccgcgagact
      901 cttgtccaga tcgagttgcg cgcgcatctg gtcgaagtac gcgacctgca gattggtgcc
      961 gatgcgcacc gtgccttcat ccggaggcaa ttcgccgagg atcatcttca gcagtgtggt
     1021 cttgcccgcc ccgttcggac cgacgaagcc gatcttgtca ccacgcatca ccgtagccgt
     1081 gaacgtgccg accaccgtgc gcgaaccata gcgcttggtc acgtcggtca gttcggcgac
     1141 tatcttgccg gatttctcgc cctgacccac gtcgagcttg acgttgccct gcacgttgcg
     1201 gcgctcggcg cgctcattgc gcatctgcac gaggcgcgcg atccggccga cgctgcgcgt
     1261 acggcgcgct tccacgccct tgcggatcca cacttcttcc tgcgccagca gcttgtcgaa
     1321 cttggccgct tccacttgct cgacttcaag ctgctgcgcc ttcctggtct ggtaggcgct
     1381 gaaattgccc ggatacgaca acaaacgccc gcgatccagt tcgacgatgc gggtagcgac
     1441 acggtcgaga aacgcgcggt cgtgggtgat gaagagcaga cacgcgcgca gcgagaccag
     1501 caggtcttca agccagcgaa taccgtcgaa gtccaggtgg ttggtcggct cgtcgagcag
     1561 cagcacgtcc ggctgcacga ccagcgcacg cgccagcgcc acgcgctttt gcatgccgcc
     1621 cgacagcgaa ccgacccgcg cctcgccatt cagaccgatc tgctgcagcg tggtggccac
     1681 gcgcgtgctc cagttccagg cgtccgaatg gtccagcgcc gattgcagcg tgttcatgcg
     1741 cgacatcagt tcgtcgtgtg cagggccttc cggctcgtcg gcgagttgat tggcgaccac
     1801 gtcgtattca tccagcagcg cgcgggcgtg ggtgaggccg gcggcgaccg cgtcgaatac
     1861 cgtgtcgtcc gtatcgaact ccggctcctg cggcacgtag acgctcgtca aaccctgctg
     1921 gcgcgtgacg aggccgtcgt caggcttgct cagatcggcg acgatcttca gcagcgacga
     1981 cttgcccgcg ccgttacggc cgatcagccc aacgcgctcg cccgcttcga gagaaaaatc
     2041 cgcgtgatcg agcaacgcga cgtgaccgaa cgccagttgg gccccggtaa tggtgtaaag
     2101 cgacatggag aattggagaa ggcagcgatg agtcggaact gctcattgta ccgggcgcgc
     2161 gggcgggtgc ctgtatgccg gaagagatag gctgaatggc cggcttgaaa aatggcgatg
     2221 acccgtacac aagtctgacg cagacaggat ccgactgcgc ttaagccatg ctcaggccgt
     2281 gctcgagcca gatacaaacg gaggacgcgg ccggacggtg cttgcacacg cccggcccgc
     2341 tcaggcgtgc aggttacttc acgtgcaccg tgatggtctt gctcatttcc ggcccatacg
     2401 aacgatgcgc gccgtcgccg aattgcagcg tcagtgtgtg gtcgcccggc ggcagcgtca
     2461 gatcggtttc ggtctggccc ttgccgaagt ggagcgattt gtcgttggcg ggaatcactt
     2521 cgcccttggg aagcggtttg ccgtcgatca acaagtgatg atggcccgtg ccttccgtca
     2581 tcgtgccggc cggcgcgatt ttcatgccgt cgacgccgaa cactacatgc accgggttgc
     2641 tgacggtcgc gccgtccgca ggctgcacga aagacacgcc ggcggcctgc gccgcgccgg
     2701 atacggcgag caatccggcc agtaccgtgc cggccaacca tttgtttctt aacatcgttt
     2761 ttctccttgt agggaaacga ggggcatgta cgccgtgcag gcgccgatgg cggagccagt
     2821 tgcctgaggc cgatcgtcgc gatccatgcc aggcgcccgg cgcgcctgtc taaaagatta
     2881 gctgcgaatc agagcataca cgctgtgtat gacaggggcc gtcaggcggg cccggagccg
     2941 tctcgggcgc tttgcggcag atgccgcatc ggcaatcggc ggtccatttg acaaattccg
     3001 gtactattgc gggtcgccgc cgtacccaaa aaaggggcag actgagcgga tcattgcatt
     3061 gaaccaaaga agagtacgtc gtgagcgaag taatcgaagt gactgaatat aagagctggg
     3121 tctgcctgat ttgcggctgg atctacaacg aagaagaagg cattcccgaa gacggcatcg
     3181 ccccgggcac gcgcttcgcc gcaattccgg acgactggcg ctgcccgctg tgcgatgtca
     3241 gcaaggcgga gttcgcggtg gtggaattct gagtgagctc gattgaaccg gggcgcgctg
     3301 cttgatgtag tgcgccgggc ggttcgtgcg gcccagcggg tgatgcccgc tgggccgttt
     3361 gcttgttggg acgggctttg ccgccctcct cgcggctggg cgtgtttgat atactctgcg
     3421 ctcgctggcg aatttggact cgcccggtct ctgcctgagg ttgctggttg gcgggattgc
     3481 agcgattatt ctggttcggt ccctctccct gtagttcaat ggatagaaca agtgcctcct
     3541 aagcgctaga tacaggttcg attcctgtca gggggaccac ctagcttccc agcatcaccc
     3601 aagtttgccc agaaaagccc cgtatgcctt gcgctgcggg gctttttgct gtcctcattg
     3661 ccaatggcaa tcaagcgacc atcaacgcag cgagacacat aagcgccatc accatgaaca
     3721 ggctggcgac gagaaagttg tatgaatagt tcatgcggcc cccggtttgt tatcgctcaa
     3781 gcatacggtg gctccgggaa gcgcattgtt aaggacggtc agttggcgga tcctccgcat
     3841 cgtcagcaac ctcaccgctt cccacccatc atcgcgtcgc aatgaaaaac gccgtgagat
     3901 ccggtagagc ggactcaccc gcgcgatgcg ggtggcgata aacgatctgg ccgacgccaa
     3961 tccgcgcggc acgccgtgag tcacccacgc catgacgaat ccgaagatca cgaaaaagac
     4021 gtcgacacca atctcgccgt agcgcgatac gcgcggaaag ctatgacgca gccagccagc
     4081 catgcccggc cacgacgcca tgagtatcga aggcgaccac gccgagcgca cgcaaggcct
     4141 ggatcgattg atacttttga tacgcctcac gcaggccgct tcggaatctc caatcccttc
     4201 gccaccgccg gacgcgcgac gaacgctacc agcacgcgct tcacattcgg aaagtcgtcg
     4261 atcccgacca gatccccggc ttcgtagaaa ccaatcagat tgcgcaccca tgggaaagtc
     4321 gcgatatcgg cgatcgagta ggcatcgccc agaatccagt cacgtcctt
//
LOCUS       DATASH010000688         2335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5651959,
            whole genome shotgun sequence.
ACCESSION   DATASH010000688 DATASH010000000
VERSION     DATASH010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5651959"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..400)
                     /locus_tag="VHV99_18380"
     CDS             complement(89..400)
                     /locus_tag="VHV99_18380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEX3637412.1"
                     /translation="MTTKQIEVSILGVPYRLACSPETEGALLEAVARVDAEMSKIRNS
                     SNVRGTDRIAVMAALSLASELLKLQSSVRHGEAFPAEEIRRTMQQMNEQLGTVIQQYS
                     M"
     gene            complement(397..753)
                     /locus_tag="VHV99_18385"
     CDS             complement(397..753)
                     /locus_tag="VHV99_18385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015001970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEX3637413.1"
                     /translation="MLTELETLSQNIGKLIAISQRHNEARLALEEQLAQSRADVEATR
                     TELALLREERNALQAERDALSAKIDDAQVRLNAILEKLPRARAHSEPDNQLDLLEPAQ
                     HEVEAESDVTRHGENA"
     regulatory      961..1218
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1309..>2335
                     /locus_tag="VHV99_18390"
     CDS             1309..>2335
                     /locus_tag="VHV99_18390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013338467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX3637414.1"
                     /translation="MQSPLARAALLAFAGLPIVVHAQGAPSSSASSVPADSSAAEPSS
                     AVLSPVVVTAERGPQALADAIPQTSLFDQQDIADTTATDLPGLLQLAPGAQISRTGGP
                     GSTTSLYLRGASSTQSLLLIDGVRVDSVSLGAPQLAQIPLDQIDHVEVVNGNVSALYG
                     SGAIGGVVQVFTKEGGDHPPRFNFSLSYGSYHTQTQQAGVNGALDKDGNTTFSISLAR
                     SKDDGFSSLNPQEAPNANPNANGYLNESISASLRHKFNDKWDAGASYFQSNGNDSYDN
                     AYGVPTDLNNLYSKVRQVSVFANGKLTDWWTTHFIVSQGDDRSVSNTNGVYSGRFDTD
                     NRQYTWQN"
BASE COUNT          435 a          721 c          694 g          485 t
ORIGIN      
        1 cgattgaatt caagacgttt gcatgaatct ttgctcatgc acctcgtttg aatccgcagc
       61 cgatctcagg accggctaca agatactttt acatgctgta ctgctgaatc acagtaccca
      121 gttgctcgtt catttgctgc attgtacgcc ggatttcttc agcgggaaat gcttctccgt
      181 gccgcacgct gctttgcagc ttaagcagtt ctgatgccag cgacagcgct gccatgacgg
      241 caatgcgatc cgtgccgcgc acgttgctgc tattgcggat cttcgacatt tcggcgtcga
      301 ctcgcgcgac tgcctcgagc aacgcgcctt ccgtttcggg cgagcaggcg aggcgatagg
      361 gcacgccgag aatcgatact tcgatctgct tggtggtcat gcattttctc cgtggcgggt
      421 cacgtcgctc tcagcttcga cttcgtgctg agccggctcg agcagatcga gctggttgtc
      481 cggctcgctg tgcgcacgag cacgcggcag cttttcgagg atcgcattca ggcgcacctg
      541 agcgtcgtcg atcttcgcgg acagcgcgtc gcgttcggcc tgcaacgcgt ttcgttcctc
      601 gcgcagcagt gcaagttccg tgcgcgtagc ttcgacgtcg gcgcgcgatt gggcgagctg
      661 ctcttcgagc gcgagtcgcg cttcattgtg gcgttggctg atcgcaatca gcttgccgat
      721 attctgtgaa agtgtttcga gttcggtgag catctgctgc gtcctctaaa gacatcgcat
      781 tttagcgcgg aatgtcgcag gttccgacaa ttgtctcgtt tcgagacgta acaacatcgc
      841 tttgtgcagg cttttatcga ccttatgatg aaaaagtact catgcgtcgg acaatagcct
      901 gcacttaaca gaacggatgg cctgcgtcgc caccattctt gacggacccc gagtccgctc
      961 ataaactgcc cgcactctgg tgcccgcgtg cgcttttcaa cggcgcacgc agttaaacgg
     1021 gaagcagggt gcgccgttca cgtgaacgcg ccaacctgcg ctgcccccgc aacggtaagc
     1081 gaccgcattc gtcatgcaag tcgatcctga ccgcgtgttg gaagttcaac tcgcgagtgt
     1141 gccactgcgc gtcacgcgtg ggaaggcgga atcgatgtgt cgccagcccg gataccggcc
     1201 agagggagag gcacgtcacg cgcgaaaatg ttaaatacgc gggcgcctcg ttacgcatcg
     1261 gcctgcgggg aagcgggccg cgcacgctat ccacaggact ttcatcccat gcagtccccc
     1321 ctcgcccgcg cggcgctgct cgccttcgcg ggcctgccga tcgtcgtgca tgcgcaaggc
     1381 gcgccttcgt catctgcttc gtccgtgccg gcggattcat cggctgccga gccgtcttcg
     1441 gcggtgctgt cgcccgtggt ggtgaccgcg gagcgcgggc cgcaagcact cgccgatgcg
     1501 atcccgcaga cgtcgctgtt cgatcagcag gatatcgccg acaccaccgc gaccgatttg
     1561 cccggtctcc tgcaactcgc gccgggcgcg caaatctcgc gcacaggtgg ccccggttcg
     1621 acgactagcc tctacttgcg cggcgcgtcg tcgacgcaat cgctgctgct gatcgacggc
     1681 gtgcgggtcg actcggtgag cctgggtgcg ccgcaactcg cgcagattcc gctcgaccag
     1741 atcgatcacg tcgaggtggt gaacggcaac gtgtcggcgt tgtacggctc gggcgcgatc
     1801 ggcggcgtgg tgcaggtgtt cacgaaagag ggcggcgacc acccgccgcg tttcaatttt
     1861 tcgctgagct acggcagcta tcacacgcaa acgcagcagg cgggcgtgaa cggcgcattg
     1921 gataaagacg gcaacacgac cttcagcatt tcgctcgcgc gatcgaagga cgacggcttc
     1981 tcttcactca atccacagga ggcgcccaac gccaatccga atgcgaacgg ctatctgaac
     2041 gagagcatct cggcttcgct gcgccacaag ttcaacgaca aatgggacgc cggcgccagc
     2101 tactttcagt cgaacggcaa cgacagctac gacaatgcgt acggcgtgcc caccgatctg
     2161 aacaacctgt atagcaaggt gcgccaggta tcggtatttg cgaacggcaa gctgaccgac
     2221 tggtggacca cgcatttcat cgtgtcccag ggcgacgatc gcagcgtatc gaacaccaac
     2281 ggtgtttata gcggccgctt cgatacggac aatcgccagt acacatggca gaacg
//
LOCUS       DATASH010000689         4207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_507104,
            whole genome shotgun sequence.
ACCESSION   DATASH010000689 DATASH010000000
VERSION     DATASH010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507104"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="VHV99_18395"
     CDS             <1..688
                     /locus_tag="VHV99_18395"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3637415.1"
                     /translation="DASYPPFESKGSDGKLVGFDIDLGNEICARLKAKCVWVENDFDG
                     MIPALKAKKFDGVLSSMSMTPQRAEQVAFSSKLFNTPTRLVANKGSGILPTADSLKGK
                     TVGVEQGTIQETYAKTYWEPKGAKVVPYQNQDQVYADLLSGRLDAALQDAVQAEIGFL
                     KTPRGAGFDFVGKDLNDPKILGEGAGIGMRKEDTDLKAKVDKAIADIIKDGTYKKIEK
                     KYFDFNVYGS"
     gene            999..2114
                     /locus_tag="VHV99_18400"
     CDS             999..2114
                     /locus_tag="VHV99_18400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014973019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HEX3637416.1"
                     /translation="MQTQTHPLIAPTLGTARNLTSFHYGPGGGQKIYIQSSLHADELP
                     GMLVSWALRRKLAALEAAGKMRGEVVIVPVANPIGLNQHFLGHLTGRFETNSAQNFNR
                     NFYELAALIQPGIEARLTDNIDANRSAIRAAMREALDEQKPANELESQRLALQKLSYD
                     ADVVLDLHCDWEAALHLYTNPDLWPEVEPLACYLDAKASLLALNSVGNPFDEIHSFCW
                     SDLRGRYGDRFPIPNGSISVTVELRGQREVSYEYAERDAQAIIEYLTSRGVIDGTPAP
                     LPALEFAATPLAGAEPIVAPISGVLVYRCEVGTWVDVGHEIADIVDPLTDRVVTLKST
                     VAGVLYARHLTRFATAGLEFARIAGAKAFRSGSLLSN"
     gene            complement(2295..2582)
                     /locus_tag="VHV99_18405"
                     /pseudo
     CDS             complement(2295..2582)
                     /locus_tag="VHV99_18405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011353840.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
     gene            2897..4066
                     /locus_tag="VHV99_18410"
     CDS             2897..4066
                     /locus_tag="VHV99_18410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015603693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX3637417.1"
                     /translation="MNKKVLTAATLAIVANAAHAQSSVTLYGVIDAGISYVNNSKTAT
                     GGHDNLVKYDDGVAQGSRWGLRGSEDLGGGLKALFVLENGFNSGNGTLGQGGALFGRQ
                     AFVGLSKDNIGSLTFGRQYSFSTDYLGGNYSTGGQTVAGNYAYHINDVDQLTSSRINN
                     AVKFSSANYSGLTFGAMYGFSNQAGAFAGAPATGTTAAPVAGSSRAYSLGVNYANGPF
                     GVGAAYTDIRYPSQATPAFSTSIANVSTGNVRDLRTFGVGGRYSFGAATLWALYTNTR
                     FEPITGGSTTFAAYEAGAKYAFTPALTAGAGYTYMHLSDANRGHWNQADLSVDYALSK
                     RTDVYALGIYQIASGRNGTQDLQAQIGSSPTSYFGASGNGADNQLAFRVGIRHKF"
BASE COUNT          837 a         1283 c         1274 g          813 t
ORIGIN      
        1 tgacgccagc taccccccgt ttgagtcgaa gggttcggac ggcaagctcg ttggcttcga
       61 tatcgacctc ggcaatgaaa tctgcgcacg cctgaaggcc aagtgcgtgt gggtcgaaaa
      121 cgacttcgac ggcatgattc cggccctgaa ggcgaagaag ttcgacggcg tgctctcgtc
      181 gatgtccatg acaccgcaac gcgccgagca ggtcgctttc tcgtccaagc tgttcaacac
      241 gccgacacgc ctcgtcgcga ataagggttc gggcatcctg ccgacggccg actcgctcaa
      301 gggcaagacg gttggcgtgg aacagggcac gattcaggaa acctacgcaa agacctactg
      361 ggaaccgaag ggcgcgaagg tcgtgccgta tcagaaccag gaccaggtct acgccgactt
      421 gctgtcgggc cgtctggacg cagcgctgca ggacgccgtg caagctgaaa tcggcttcct
      481 gaagacgccg cgcggcgcgg gcttcgactt cgtcggcaag gatctgaacg atccgaagat
      541 cctcggcgaa ggcgcgggca tcggcatgcg caaggaagac accgatctga aggcgaaggt
      601 cgacaaggcg atcgccgaca tcatcaagga cggtacgtac aagaagatcg agaagaagta
      661 cttcgacttc aacgtgtacg gcagctaagc cagccaggcg cgtctgacgg cgcgcttgcc
      721 ggcttgcaga aacgggctcc gctacatgcg gggcccgttt cgtttgaggg gccgctggcc
      781 gttccgcctc ggctacgctc cggccgaacc gggccgcccg catgccacgc gcgatccgca
      841 caatgccgga taagaccccg catcgcgtca tatcggctaa gatcgtgcga tagcggcgcc
      901 gcgagccggc ccgccaatga tcggctatga agcgcccttt ccccctctaa tcaacgacat
      961 agcgcggcat gccccgcttg cccgcggatt tgaaaaccat gcaaacccaa acccatccgc
     1021 tgattgcccc gacgctcggc accgcccgta atctgacgag ttttcactac ggtcccggcg
     1081 gcgggcagaa gatttacatc cagtcgtcgc tgcatgccga cgagttgcct ggcatgctgg
     1141 tctcgtgggc attgcgccgc aaactggccg cgctggaagc ggccggcaag atgcgcggcg
     1201 aagtcgtgat cgtgccggta gccaatccga tcggcttgaa tcagcatttt ctcggccatc
     1261 tgacgggccg cttcgagacc aacagcgcgc agaacttcaa ccgcaatttc tacgaactcg
     1321 cggcgctgat tcagcccggt atcgaagcgc gtctgaccga caacatcgac gccaatcgca
     1381 gcgcgatccg cgcggcgatg cgtgaggcgc tcgatgagca aaagccggca aacgagctcg
     1441 aatcgcaacg tctggcgctg caaaagctct cgtatgacgc cgatgtcgta ctcgacctgc
     1501 actgcgactg ggaagccgct ctgcacctgt acaccaatcc cgacctgtgg ccggaagtcg
     1561 agccgctggc ctgctatctg gacgccaagg cgtcgctgct ggcactgaat tcggtgggca
     1621 atccgttcga cgaaatccac agcttctgct ggtcggatct gcgcggccgt tacggcgatc
     1681 gctttccgat tccgaacggc tcgatctcgg tcacggttga actgcgcggt caacgcgaag
     1741 tgtcgtacga gtacgccgag cgcgacgctc aggcgatcat cgaatacctg acctcgcgcg
     1801 gcgtgatcga cggcacgccg gcgccgctgc cggcgctcga attcgcagcc acgccgctgg
     1861 ccggcgccga accgatcgtc gcgccgatca gcggtgtgct ggtgtatcgc tgtgaagtgg
     1921 gtacctgggt cgatgtcggc catgagatcg ccgacatcgt cgatccgttg accgatcgcg
     1981 tcgtgacgct gaagagcacc gtcgccggtg tgctgtacgc gcggcatttg acgcgctttg
     2041 cgacggccgg gctggaattc gcgcgcattg ccggtgcgaa ggcgtttaga agcggctcgt
     2101 tgttgagcaa ctgaggagtc tttgacgaac accgcaggcc gggcttgccc gtattagcct
     2161 gcggaagcta tgcccgcggc aagcagatcg cccgctattt cagcgggcga tctgcgttta
     2221 cgcatctgtg gcgaagcagc agatttgctc aaaggcggct ggaatcccac atatagacct
     2281 gctcaggaac cagctcaaaa agccggaatg atcgcgcctt tgaactgtgt atcgatgtac
     2341 ttgcgcacgt cgtccgattc ataagcggca accagcttct tcacccacgg cttgtcgcga
     2401 tcctgcgcgc gcacggcgat cagatttgcg tacggaccct tgaggtcttc gatcgcgatc
     2461 gcgtcttttg tcggctgcaa gccggccttc accgcgtagt ccgtgttgat cgacgccgcg
     2521 tcgaggtcgt cgagcgcgcg cggcaattgc gcggcgtcga gttccaccag cttgatcttc
     2581 ttgattttcc ggcgttgcat gcatttcccc acaagcgatt tcgattgata cgcgcaaatc
     2641 gtcgcatgca gccgatgcgt ccgaaaccat gcgtttttca cacgcttata tgcacaatct
     2701 ggaaaaagct tatggcgggc atgtcttgcg tatttcaatt cgcgacgcga tgtgagtgtc
     2761 gtgccgatgc gacagattac aaaaataaaa gggatgtcac ataccttact cagcattccc
     2821 ttaaatttcg ggccatcgca cgaccctgcc gcagagcacc gcgtcgcgat cgtttaattt
     2881 ttattcggag aaagtcttga acaagaaagt attgaccgcc gctactctcg ccatcgtcgc
     2941 caacgccgcc cacgcgcaaa gcagcgtcac gctgtacggc gtgatcgatg ctggtatcag
     3001 ctacgtgaac aattcgaaga ccgctaccgg cggtcacgac aacctcgtca agtacgacga
     3061 cggtgttgct caaggcagcc gttggggtct gcgcggcagc gaagacctcg gtggtggcct
     3121 gaaggcgctg ttcgtgttgg aaaacggctt taacagcggc aacggcaccc tcggccaggg
     3181 tggtgcgctg ttcggccgtc aagcgtttgt cggtctgtcg aaggacaaca tcggctcgct
     3241 gacgttcggt cgccagtatt cgttttcgac cgactatctg ggtggcaact actcgaccgg
     3301 cggtcagacc gtcgcaggta actacgctta ccacatcaac gacgtcgacc agttgacgtc
     3361 gagccgtatc aacaacgcag tcaagttcag cagcgcgaat tactcgggcc tgaccttcgg
     3421 cgcgatgtac ggcttctcga accaggctgg cgcattcgca ggcgcgccgg ctaccggcac
     3481 gaccgctgca ccggtggcgg gttcgtcgcg tgcgtacagc ctgggcgtga actacgcgaa
     3541 cggcccgttc ggcgtaggcg cggcgtacac ggatatccgt tacccgagcc aggcaacccc
     3601 ggcgttctcg accagcatcg ccaacgtgtc gacaggcaat gtgcgcgatc tgcgcacgtt
     3661 cggtgtcggc ggccgctact cgttcggcgc agctacgctg tgggcgttgt acacgaacac
     3721 gcgcttcgag ccgatcacgg gcggttcgac cacgttcgcc gcatacgaag cgggcgcgaa
     3781 gtacgcgttc acgccggcac tgacggcagg cgcgggctac acgtacatgc atctgagcga
     3841 cgcgaatcgt ggccattgga accaggccga cctgagcgtc gactatgcat tgagcaagcg
     3901 tacggacgtc tacgcactgg gcatctacca gatcgcttcg ggacgcaacg gtacgcagga
     3961 tctgcaagca cagatcggtt cgagcccgac gtcgtacttc ggcgcctcgg gcaacggtgc
     4021 ggacaaccaa ctggcgttcc gcgtcggtat ccgtcataag ttctaagcga gtcttgcttt
     4081 gaccggcgag gagcgcgagt tccttgccgt gccaaggtga tgaaaaacgc cctgcggggc
     4141 gtttttcttt tggggaatcg gcttttcagg tacttgcgtc gatgcaaaaa agagtgattc
     4201 acgctgt
//
LOCUS       DATASH010000690         1802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5652239,
            whole genome shotgun sequence.
ACCESSION   DATASH010000690 DATASH010000000
VERSION     DATASH010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5652239"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="VHV99_18415"
     CDS             <1..810
                     /locus_tag="VHV99_18415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012405776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3637418.1"
                     /translation="FSVLGLIGLVLVVFWMTIAVIGPWIAPYKSGALSSVDVFAGSSH
                     AFLLGTDYLGRDMLSRILYGTRYTVGLALAAALMASLIGTFFGLIAAVSGRWVDEILS
                     RLFDALISIPSKVLALVVIAAFGSSIPMLMGVAALAYIPGAFRISRSLAVNLMTLEYV
                     QVARARGEGLFYIARVEVLPNMIHPMLADFGLRFVFIVLLLSGLSFLGLGVQPPNADW
                     GSLVRENIGGLAEGAPAVLMPAIAIATLTIGVNLLIDNLRRRGGRGQGAAQ"
     gene            807..>1802
                     /locus_tag="VHV99_18420"
     CDS             807..>1802
                     /locus_tag="VHV99_18420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3637419.1"
                     /translation="MKADTIDMIQVKGLRVVAGAAPDPVTEIVKGVNFSVKKGEVLAL
                     IGESGSGKTTIALALLGYARQGCSISGGSVRIGETEVLELDQRGRRALRARTVAYVAQ
                     SASSGFNPARTIIDQVTEPALLHRLMTKEAARRKAIGLFRALALPEADTIGERYPHQV
                     SGGQLQRLMAAMALITDPAVVVFDEPTTALDVTTQIEVLAAFKKVIRELGTTAVYVSH
                     DLAVVAQMADRIVVLNGGRVKEDGLVAQVLDTPVDAYTQQLLAATRRPEPVLPMQTAA
                     QKLAPPLLEIRGLAAGYGRIDRYGAPAVRVLDDVSLSIARGSTLGVIGESGSGKTT"
BASE COUNT          292 a          526 c          627 g          357 t
ORIGIN      
        1 ttctccgtgc tcggtttgat cggcctcgtg ctggtcgtat tctggatgac cattgcggtc
       61 atcggtccgt ggatcgcgcc ttacaaaagc ggtgcgctgt catccgtgga tgtgttcgcc
      121 gggtcgagcc atgcgttcct gctgggcacc gactatctcg gccgcgacat gctgagccgg
      181 attctctacg gcacccgcta tacggtcggg ctggcgctgg cagcggcgtt gatggcaagc
      241 ctgatcggca cgttcttcgg cttgatcgcc gctgtgtccg gacgttgggt cgatgaaatt
      301 ttgagccgcc tgttcgacgc gttgatctcc attccgagca aggtgctcgc gctcgtcgtg
      361 atcgccgcgt ttggctcgtc cattcccatg ctgatgggtg tggccgcgct cgcctacatt
      421 cccggcgcgt tccgcatctc gcgctcgctc gcggtcaacc tgatgacgct ggaatacgtg
      481 caggtcgcgc gggctcgcgg cgaagggctg ttctacatcg cacgcgtgga agtgctgccc
      541 aacatgatcc atcccatgct cgccgacttc ggcctgcgct tcgtattcat cgtgttgctg
      601 ctcagcggcc tgagcttcct gggcctcggc gtgcagccgc ccaatgccga ctggggatcg
      661 cttgtgcgcg agaacattgg cggactggct gaaggggcgc ccgccgtctt gatgccggcg
      721 attgcgatcg cgacactgac tatcggcgtg aatctgctga tcgacaacct gcgccgccgt
      781 ggcggacgcg ggcaaggagc tgcgcaatga aagccgacac gatcgacatg attcaagtga
      841 agggcttgcg agtggtggca ggcgccgcac ctgatccggt gacggagatc gtcaagggcg
      901 tcaacttttc ggtgaagaaa ggcgaagtgc tggcgttgat cggcgagtcg ggatcgggca
      961 agacgaccat cgcgctggcg ctgctgggat atgcgcggca agggtgttcg attagcggcg
     1021 ggtcggtgcg gatcggcgag acagaagtgc tcgaactcga ccagcgaggc cgccgggcgc
     1081 tgcgtgcgcg gaccgtggcg tatgtggcgc agagtgcatc ttccgggttc aatccggcgc
     1141 gtacgattat cgatcaggtg acggaacccg cgctgctgca tcgcttgatg acgaaagagg
     1201 cggcacgtcg caaggcgatc ggattgtttc gtgcgcttgc gttaccggag gcggacacga
     1261 tcggcgagcg ttatccgcat caggtctcgg gcgggcagtt gcagcgcctg atggcggcca
     1321 tggcgttgat cacggaccca gcagtggtcg tgttcgacga gccgacgacc gcactcgatg
     1381 tgaccacgca aatcgaagtg cttgcagcgt tcaagaaggt gatccgcgag ctgggcacca
     1441 cggctgtcta cgtgtcgcac gatctggcgg tggtcgcgca aatggcggac cggattgtcg
     1501 tgctgaacgg cggccgggtg aaagaagacg gtctggtggc gcaagtgctc gacacgcccg
     1561 ttgacgcgta cacgcagcag ttgctggctg ccacgcgacg gccggaaccg gtgttgccga
     1621 tgcagacggc cgctcagaaa ctcgcgccgc ccttgctcga aatccgcgga ctggcggcgg
     1681 gttacggccg tatcgaccgg tatggcgcgc cggccgtgcg cgtgctcgac gacgtgagcc
     1741 tgtccattgc acgcggcagc acgctgggcg tgatcggcga atcgggatcg ggcaagacga
     1801 cg
//
LOCUS       DATASH010000691         2209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4465735,
            whole genome shotgun sequence.
ACCESSION   DATASH010000691 DATASH010000000
VERSION     DATASH010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4465735"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..>2209)
                     /locus_tag="VHV99_18425"
     CDS             complement(182..>2209)
                     /locus_tag="VHV99_18425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX3637420.1"
                     /translation="VLAGAGSGKTRVITQKIAHLIEAKGFEPRHIAAVTFTNKAAAEM
                     RERVGKLLEGKTLTTPGKEGRKVPVNQLTVCTFHSLGVQILRQEAEHVGLKPQFSIMD
                     SDDCFGMIQEQVGSTDKGFIRKIQSIISLWKNGLIMPEEAIAIAASEDEHQAAIVYRN
                     YVATLHAYQAVDFDDLIRLPAELFAKNEQVRDRWQNKLRYLLIDEYQDTNACQYELVK
                     LLAGKRAAFTAVGDDDQAIYGWRGATLENLGQLSKDFPKLHLIKLEQNYRSTVRILTA
                     ANNVIANNPKLFEKKLGSEHGMGDTITVTPCNDEEHEAESVVFRLSAHKFERRANFRD
                     YAILYRGNFQARIFEQVLRRERIPYVLSGGQSFFDKAEIKDICAYLRLIANANDDPAF
                     IRAITTPRRGVGNTTLEALGSFAGQAKVSLFEAVYMGGIEARLSPRQIEPMRVFCDFM
                     QRLTDRAEKDAAGPLLDELMDAIHYEAYLYDAFDERQAQAKWQNVLEFMEWLKRKGTK
                     AEPEGSANSEATGYDTADGLGDTGKNLLGLIQTVALMSMLEGREEDPDAVRLSTVHAS
                     KGLEYPHVFLVGVEEGIMPHRGGPDDEPIDDARIEEERRLMYVAITRAQRSLHLNWCK
                     KRKRARETVVCEPSRFIPEMLLDDAPPPTPEEAPMSPKDRLASLKALLQKP"
BASE COUNT          367 a          697 c          686 g          459 t
ORIGIN      
        1 gtgccactgt agcgtgcgca cacgggaaaa tcgccgatgc acctgcagtg cgcggggtct
       61 tgtaagcgtg tgggagcgtc cggcgcgtcg ggtcttgcaa gcaagtagct gtgcccggcg
      121 cgctgggtct tacaggcaag cgaacagaaa atgcccatct ggctcacgtt cgcctccacg
      181 ctcaaggctt ctgcaacagc gccttcaaac tcgcgagccg gtctttcggc gacatcggcg
      241 cttcttcggg tgtcggcggc ggagcgtcgt cgagcagcat ctcggggata aagcgcgacg
      301 gctcgcacac cacggtctcg cgcgcccgct tgcgcttctt gcaccagttc agatgcaggc
      361 tgcgctgcgc acgcgtgatc gcgacgtaca tcagacgacg ctcttcctcg atgcgcgcgt
      421 cgtcgatcgg ctcgtcgtcg gggccgccac ggtgcggcat gatgccttct tcgacgccca
      481 ccaggaacac gtgcggatac tccagcccct tggacgcatg gaccgtcgag agccgcaccg
      541 cgtccggatc ttcttcgcgg ccttcgagca tcgacatcaa cgccacggtc tggatcaggc
      601 ccagcagatt cttgccggtg tcgccgaggc cgtcggcggt gtcgtagccg gtcgcttcgc
      661 tgttcgccga accctcgggc tcagccttgg tgcccttacg cttcaaccac tccatgaact
      721 cgagcacgtt ctgccacttg gcctgcgcct gacgttcgtc gaacgcgtcg tacagatagg
      781 cttcgtaatg gatcgcgtcc atcagttcgt cgagcaaggg accggcggca tctttctcgg
      841 cacgatcggt gagacgctgc atgaagtcgc agaacacgcg catcggctcg atctgacgcg
      901 gcgacaggcg cgcctcgatg ccgcccatgt acaccgcttc gaatagcgac accttcgcct
      961 ggcccgcgaa cgagccgagc gcctcgagcg tcgtattgcc gacgccgcgg cgcggcgtgg
     1021 tgatcgcgcg gatgaacgcg gggtcgtcat tcgcgttggc gatcaggcgc agatacgcgc
     1081 agatatcctt gatctcggct ttgtcgaaga acgactggcc gcccgacagc acatacggaa
     1141 tccgttcgcg acgcagcacc tgttcgaaga tgcgcgcctg gaaattgccg cgatacagga
     1201 tcgcgtagtc gcggaaattc gcgcgccgct cgaacttgtg cgcggacagg cggaacacga
     1261 cggactcggc ctcatgctcc tcgtcgttgc aaggcgtgac ggtgatcgtg tcgcccatgc
     1321 cgtgttcgga ccccagcttc ttttcgaaca gtttcgggtt gttcgcaatc acgttgttcg
     1381 cggcggtgag aatgcgcacc gtcgaacggt agttctgttc cagcttgatc agatgcagct
     1441 tcggaaagtc cttgctgagc tggccgagat tttcgagcgt cgcgccgcgc cagccgtaga
     1501 tcgcctgatc gtcgtcgccg accgccgtga acgccgcgcg cttgccggcc agcagtttga
     1561 ccagctcgta ctggcacgcg ttggtgtcct gatactcgtc gatcagcaga tagcgcagct
     1621 tgttttgcca gcggtcacgt acctgctcgt tcttggcgaa tagttcggcg ggcaggcgga
     1681 tcaggtcgtc gaaatcgacc gcctgataag catgcagtgt cgccacatag ttgcgataga
     1741 cgatcgcggc ctgatgttcg tcttcgctcg cggcgatcgc gatcgcctct tccggcatga
     1801 tcaggccgtt tttccacagc gagatgatcg actggatctt gcggataaaa cctttatccg
     1861 tcgagccgac ctgttcctgg atcatgccga agcagtcgtc cgaatccatg atcgagaact
     1921 gcggcttgag gccgacgtgt tccgcttcct gccgcagaat ctgcacgccg agcgagtgga
     1981 aggtgcagac cgtcaactga ttgacgggca ccttgcggcc ttccttgccg ggcgtggtga
     2041 gcgtcttgcc ttcgagcagc ttgccgacac gctcgcgcat ttccgccgca gccttgttcg
     2101 tgaacgtgac ggcggcgatg tggcgcggct cgaagccttt ggcttcgatc aggtgggcga
     2161 ttttctgcgt gatcacgcgc gtcttgccgc tgcctgcgcc ggcgagcac
//
LOCUS       DATASH010000692         3649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5253886,
            whole genome shotgun sequence.
ACCESSION   DATASH010000692 DATASH010000000
VERSION     DATASH010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5253886"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2895
                     /locus_tag="VHV99_18430"
     CDS             <1..2895
                     /locus_tag="VHV99_18430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HEX3637421.1"
                     /translation="SGAEPIERYRFKGDLQGISVAAQEPPPGLTPLNHPRAGIPGIEN
                     LWGRVDADENHGTAMLDTSNVAITLPGVFDDPRLKFDRLRGQADWTIAPKAPSENHPA
                     FTVKLANFGVSNKDADATATANYSNPGHGRGSLDLKADFQRAQVTRIVRYLPTSISEK
                     LRIYLGHGLQAGMSRGATIEIHGDLTKFPYSRAPEAGIFHIVAPFKGGKFDPSPYPPR
                     KMRNGTPNVWPPLDGIDGMFELKQNVLRFDIDRAHYKRVALTRVNGKIDDLGTRASNL
                     VIDGDGRGPLADMLDYVNESSLGIMAKHETEKVRAEGSASLALKLTVPRTPKPHIGVE
                     GAVGFQNNRLTVENVPPLSQLNGKVRFTEHTAQVERLSGQFLGGDVHANGGLKQDGTY
                     ALELGGHIAVDAARGLNLHGPATQVLTRMNGSAPYALNVRGAKGRLPEVTANSDLTGL
                     GLDFPAPFNKPVGTPMPLHFALSPATDASEAGLERADLTFGPIAATYLLRYEPKAQPK
                     TPPTVVRGAIGVNKPADLPSNGVIAAVDVEAFDADAWRALVTQMRNKEAPTPGSAGVA
                     APAAAPAVPDPFLAQFLPSRFALHIESLTLLKRHWDSVIVGASHADGKWQANIASNQV
                     SGHVSWLPGASKESPGTLQARFARVVIPSATDKDLLGQAMSAPAQNMPSIDLVVNELI
                     VRDRNIGRLEVDAHNFEEDGGPVWQLDKLDITNPAATLTATANWRTSTGLGNAADETA
                     QRRTLFDFKLDIKDAGALLERFGQPRTLKAGSGTLSGRVVWHGGPTAIDYPTFNGNLA
                     VDLRHGEILKVDPGVAKLLGVLSLQSLARFVTLNFRDVIGEGLPFEHVTGTGQIHNGI
                     GRTENFEMVTAPARAEMKGSVDLAQETQDLHVRIVPTVSAGAAVIAATVINPLLGLGA
                     LVADLAFSKSVSTAFAREYAITGPWSKPHIERVKSDRGKMDAPASTVEVH"
     gene            3030..>3649
                     /locus_tag="VHV99_18435"
     CDS             3030..>3649
                     /locus_tag="VHV99_18435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX3637422.1"
                     /translation="MSETHVFSTASSAASSAVASNGSSGSTFRVAALQMVSTPDRERN
                     LAEAERLIGQAAAEGAQLVQLPEYFCFMGFKDTDKLAVREPYQDGPIQRFLADAARRH
                     KVWVIGGTLPLMSGEPSRVLNTTLVFDPQGNEAARYDKIHLFNFEKGEESFDEARTIC
                     PGGEVRTFEAPFGRVGLSVCYDLRFPELYRRMGDCALIVVPSAFTYT"
BASE COUNT          671 a         1250 c         1148 g          580 t
ORIGIN      
        1 agcggcgccg agccgatcga gcgctatcgt ttcaagggcg atctgcaagg catcagcgtc
       61 gccgcgcagg aaccgccgcc ggggctcacc ccactgaatc acccgcgcgc gggcattccc
      121 ggcatcgaaa acctgtgggg ccgcgtggac gccgacgaaa accacggcac ggcgatgctc
      181 gacacctcca acgtcgcgat tacgctgccg ggtgtgttcg acgacccgcg cctgaagttc
      241 gaccgtctgc gcggccaggc agactggacc atcgcgccga aggcacccag cgaaaatcac
      301 ccggccttca ccgtcaagct ggccaacttc ggcgtttcga acaaggacgc cgatgccact
      361 gccaccgcca actacagcaa cccagggcac gggcgcggtt cgctcgatct gaaagccgac
      421 ttccagcgcg cccaggtcac gcgcatcgtc cgctacctgc cgaccagcat cagcgaaaag
      481 ctgcgcatct accttggcca cgggctgcaa gccggcatgt cgcgcggtgc gacgatcgaa
      541 atacacggcg atctgaccaa attcccctac tcccgcgccc cggaagccgg gattttccat
      601 atcgtcgcgc cgttcaaggg cggcaagttc gacccttcgc catacccgcc gcgcaagatg
      661 aggaacggca cgccgaacgt gtggccgccg cttgacggca tcgacggcat gttcgaactc
      721 aagcagaacg tgctgcgctt cgatatcgac cgcgcccact acaagcgggt cgcactgacg
      781 cgtgtcaacg gcaagatcga cgatctcggc accagggcgt ccaacctcgt gatcgatggc
      841 gacggccgtg ggccgctcgc cgacatgctc gactacgtga atgaaagctc gctcggcatc
      901 atggccaaac atgagaccga gaaggtgcgc gctgaaggat ccgcgtcgct ggcgctcaaa
      961 ctgaccgtac cgcgcacgcc gaagccccat atcggcgtgg aaggcgcggt cggttttcag
     1021 aacaatcgct tgactgtcga aaacgtgccg ccgctgtcgc aactgaacgg caaggtgcgc
     1081 ttcaccgaac acacggcaca ggtcgagcgg ctctcgggcc agttcctcgg cggcgatgtg
     1141 cacgcgaatg gcggcctgaa gcaggacggt acgtatgcgc tcgagctggg cggccacatt
     1201 gccgtcgacg ccgcgcgcgg tctgaatctg catggaccgg ccacgcaggt gctgacgcgc
     1261 atgaacggca gcgcgcccta cgcgctcaac gtgcgcggcg ccaaagggcg tttgccggag
     1321 gtcacggcga actccgatct gaccggcctc gggctcgact tccccgcgcc gttcaacaaa
     1381 ccggtcggca ccccgatgcc gctgcacttc gcgctcagcc cggccaccga cgcgagcgaa
     1441 gccggtctgg aacgcgccga cctcaccttc ggcccgatcg ccgccaccta tttgctgcgt
     1501 tatgagccaa aagcgcagcc gaaaacgccg ccgacagtgg tgcgcggagc gatcggcgtc
     1561 aacaagcccg ccgacttgcc gtccaacggt gtgatcgccg cggttgacgt cgaagcgttc
     1621 gacgccgacg cctggcgcgc gctcgtcacg cagatgcgca acaaggaagc gcccacgccc
     1681 ggttccgccg gcgtagccgc tcccgccgcc gcgccggctg tgccggatcc gtttctcgcg
     1741 caatttctgc cgagccgctt cgcgctgcac atcgagtcgc ttacgctgct caagcggcac
     1801 tgggacagcg tgatcgtcgg cgcatcgcac gcggacggca aatggcaggc aaacatcgcg
     1861 tcgaaccagg tgtcggggca cgtttcgtgg ctgccgggcg ccagcaagga atcgcccggc
     1921 acgttgcagg cgcgctttgc ccgcgtggtg atcccttcgg ccaccgacaa ggacctgctc
     1981 ggccaggcga tgtcggcgcc ggcgcagaac atgccgtcca tcgatctggt ggtcaacgag
     2041 ttgatcgtgc gcgatcgcaa cattggccgg ctcgaagtcg acgcgcacaa cttcgaggaa
     2101 gacggcggac cggtctggca actcgacaag ctcgacatca ccaaccccgc cgccacgctg
     2161 accgcaaccg cgaactggcg cacctcgacc ggtctcggca acgccgccga cgaaacggca
     2221 caacgccgta ccctgttcga tttcaaactc gacatcaagg acgccggcgc gttgttggag
     2281 cgcttcggcc agccgcgcac gctcaaggcc ggcagcggca cgctgtcggg cagggtcgtg
     2341 tggcacggcg gcccgaccgc catcgactac ccgacgttca acggcaatct ggccgtcgat
     2401 ctgcgccacg gagagattct caaggtcgat ccgggcgtcg cgaaactgct cggcgtgctc
     2461 agcctgcaaa gcctcgcgcg cttcgtcacg ctcaatttcc gcgatgtgat cggcgaaggg
     2521 ctgccgttcg agcacgtcac cggcacgggt cagatccaca atggcattgg ccgtactgag
     2581 aacttcgaga tggtgacggc gccggcccgt gctgagatga agggctcggt ggatctggcg
     2641 caggagaccc aggatctgca cgtgcggatc gtgccgaccg tcagcgccgg cgccgccgtg
     2701 atcgccgcga ccgtgatcaa tccgctgctc ggtctgggcg cgctggtggc cgatctggcg
     2761 ttcagcaagt cagtctccac ggcgtttgcg cgcgaatacg cgatcaccgg tccgtggtcg
     2821 aagccgcaca ttgagcgggt gaagagcgat cgcggtaaga tggacgctcc ggcttcgacc
     2881 gtggaagtgc actgagcctt cagcagcctt tggggccgtt gatcatgata gagcgcagtg
     2941 ccccacaggc cccagccggc cttgaacgac gtatgcggca tgagcagttc tcgcgccgcc
     3001 gcctttgccg ggagtttttc gaaacgctca tgagcgaaac acacgtcttt tccaccgctt
     3061 cgtccgcggc ctcatccgcc gtagcgtcca acgggtcttc cggcagcacc ttccgcgtgg
     3121 ccgcgctaca aatggtcagc acgccggatc gcgagcgcaa tctggccgaa gcggaacgcc
     3181 tgatcgggca agccgccgcc gagggcgcgc aactggtcca actgcccgag tatttctgct
     3241 tcatgggctt caaggacacg gacaagctcg ccgtgcgcga gccctaccag gacggcccaa
     3301 tccagcgctt tctcgccgat gccgcgcgcc gtcacaaggt gtgggtgatc ggcggcacgt
     3361 tgccgttgat gtcaggggaa ccgtcgcggg tgctgaacac cacgctggtg ttcgacccgc
     3421 aaggcaacga ggccgcgcgc tacgacaaga tccatctgtt caacttcgaa aagggcgagg
     3481 aatcgttcga cgaggcacgc accatctgcc ccggcggcga agtccgcacc ttcgaggcgc
     3541 cgttcggccg ggtcggcctg tcggtctgct acgatctgcg ctttccggag ctgtaccggc
     3601 gcatgggcga ctgcgcgctg atcgtggtgc cgtcggcgtt cacctacac
//
LOCUS       DATASH010000693         1919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2090516,
            whole genome shotgun sequence.
ACCESSION   DATASH010000693 DATASH010000000
VERSION     DATASH010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2090516"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="VHV99_18440"
     CDS             <1..790
                     /locus_tag="VHV99_18440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX3637423.1"
                     /translation="DLAYHPLVQQRYPVLSSAILAGASQQLRNMASTGGNLLQRTRCA
                     YFYDTATPCNKREPRSGCSAIDGINRMHAIFGASDSCIAVHPSDMCVALAMLEATVHV
                     TGASGERAIAFADFHRLPGDTPQRDTNLGTDEIITAVELPPRGFAANHTYLKIRDRLS
                     YAFALVSVAVGLELERDTIKEARLALGGVAHRPWRNALAEAELRGQPARPAAFTQAAD
                     VMLRDAKGFEHNSFKIDLARRCIVRALSQAARGVAQSQSIKKIA"
     gene            799..>1919
                     /locus_tag="VHV99_18445"
     CDS             799..>1919
                     /locus_tag="VHV99_18445"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX3637424.1"
                     /translation="MASYIGTATSRVDGHAKVTGAAKYAAEFDAPDLVHASVVSSTIT
                     HGRIVHIDTSAALRVPGVIEVLTHEHRPHMADNDAGYHDDVAPDGTPFRPLYDDKVRF
                     NGQPIAVVIAEESETARFAASLVRVEYAQEAHATDLERERDNTVALADDTFSLTPSKP
                     RGNAEKALAAAAVRHDAEYRIAIEHHNPMEPYASTVIWQGGGKLTVHDKTQGVQNVQR
                     YLCGVFEMPSDDIRVVSPFVGGAFGSGLRPQYNAVLAVLAALAVQRSVRLVLTRAQMY
                     SLGYRPESIERVTLGADADGTLSAITHEAIAMTSRFEEFARKDTIWSGMLYTCPNTRY
                     AHRLAPLDVNTPCDMRAPGAATGVYALECAMDELAVALGL"
BASE COUNT          353 a          666 c          600 g          300 t
ORIGIN      
        1 cgacctcgcc taccacccgc tggtgcagca acgatatccg gtgctgtcga gcgcgatcct
       61 ggccggggcg tcgcagcaac tgcgcaatat ggcgtcgacc ggcggcaacc tgctgcaacg
      121 cacgcgctgc gcttacttct acgacacggc aacgccctgc aacaagcgcg aaccccgcag
      181 cggctgctcc gccatcgacg gcatcaaccg catgcacgcg atcttcggtg ccagcgattc
      241 ctgcatcgcc gtgcatcctt ccgacatgtg cgtggcgctc gccatgctgg aggcaacggt
      301 gcatgtgaca ggtgcatcag gcgagcgcgc cattgcgttc gccgacttcc atcggctgcc
      361 gggcgatacg ccacagcgcg acaccaacct cggcaccgat gagatcatca ctgcggtgga
      421 actgccgccg cgcggttttg ccgccaacca cacctacctc aagatccgcg accgcctgtc
      481 ctacgccttc gctttggtat ccgtggcggt cgggctcgag ctcgagcggg ataccatcaa
      541 ggaggcgcgc ctcgcacttg gcggtgtcgc acacaggccc tggcgcaatg cgctggccga
      601 agccgaactc cgtggccagc cggcccgccc cgcggcattc acccaggcgg cggacgtcat
      661 gctgcgtgat gccaaaggct tcgagcacaa cagcttcaag atcgatctcg cgcgacgatg
      721 catcgtgcgc gcgctgtcgc aggcggcgcg cggcgtggcg caatcgcaat ccatcaagaa
      781 aatcgcgtga gcaggcacat ggcatcctac atcggcaccg ccacatcccg cgttgacggc
      841 cacgccaaag tcaccggcgc ggcaaaatat gccgcggaat tcgacgcacc ggacctcgtc
      901 catgccagcg tcgtgtcatc caccatcacg cacggccgca tcgtgcacat cgacaccagt
      961 gcggcgttgc gcgtccccgg cgtgatcgag gtgctgacgc acgagcaccg cccgcacatg
     1021 gcggacaacg atgcaggcta ccacgacgat gtggcgccag acggcacgcc gttccggccg
     1081 ctttacgatg acaaggttcg gttcaacggc cagccgatcg cggtggtgat cgccgaagaa
     1141 tccgagaccg cacgcttcgc ggcctcgctg gtgcgcgtcg aatacgccca ggaggcccac
     1201 gccaccgacc tggagcgcga acgcgacaat accgttgcac tggccgacga cacgttctcc
     1261 ctgaccccgt caaaaccgcg cggcaacgcc gagaaggcgc ttgccgccgc cgcggtgcgc
     1321 cacgacgccg agtaccgcat cgcgatcgaa caccataatc cgatggagcc gtatgcctcc
     1381 acggtgatct ggcagggtgg cggcaagctg acggtgcatg acaagacgca gggcgtgcag
     1441 aacgtgcagc gctacctgtg cggcgtgttc gaaatgccgt ccgacgacat ccgggtggtc
     1501 tcaccgttcg tcggcggcgc gttcggcagc ggcctgcggc cgcagtacaa cgcggtcctc
     1561 gcggtgctgg cagcactcgc cgtgcaacgg tcggtgcgac tcgtgctgac ccgggcgcag
     1621 atgtattcgc ttggctatcg tcccgagagc atcgagcggg tcacactcgg cgcggatgcg
     1681 gacggcacgc tctcggcgat cacccacgaa gcgatcgcta tgacgtcgcg gttcgaagaa
     1741 ttcgcccgga aagatacgat ctggtcgggc atgctgtaca cgtgccccaa cacgcgatac
     1801 gcacataggc tggcaccgct cgatgtcaac acgccgtgcg acatgcgtgc gcccggtgcg
     1861 gcgaccggcg tctatgcgct ggaatgcgcg atggacgaac ttgctgtcgc gctggggct
//
LOCUS       DATASH010000694         4510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2880374,
            whole genome shotgun sequence.
ACCESSION   DATASH010000694 DATASH010000000
VERSION     DATASH010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2880374"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            258..1751
                     /gene="trpE"
                     /locus_tag="VHV99_18450"
     CDS             258..1751
                     /gene="trpE"
                     /locus_tag="VHV99_18450"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEX3637425.1"
                     /translation="MTELEFQSLANEGFNRIPLIAEALADLETPLSLYLKLAQPERNG
                     ANSFLLESVVGGERFGRYSFIGLPARTLLRTRNGVSEVLRDGKVVETHEGDPLEFIQQ
                     FQGRFKVAQRPGLPRFAGGLAGYFGYDAVRYIEKKLAHTAPKDDLNLPDIQLLLTEEV
                     AVIDNLAGKLYLVVYADPTQPEAYTKARQRLRELRQRLRTTVQPPVTSASVRTETYRE
                     FAKDDYLAAVRKAKEYIAAGELMQVQVGQRLIKPYRDNPLSLYRALRSLNPSPYMYYY
                     NFGDFHVVGASPEILVRQEKRGEDRIVTIRPLAGTRPRGNTPERDAELATELLNDPKE
                     IAEHVMLIDLARNDVGRIAQIGSVVVTDKMVIEKYSHVQHIVSSVEGKLKPGTTNFDV
                     LRATFPAGTLSGAPKVRAMELIDELEPVKRGLYGGAVGYLSFTGEMDLAITIRTGVIA
                     NGNLYVQAAAGVVADSVPESEWQETENKARAVLRAAEQVQDGLDSDF"
     gene            1765..2352
                     /locus_tag="VHV99_18455"
     CDS             1765..2352
                     /locus_tag="VHV99_18455"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEX3637426.1"
                     /translation="MLLMIDNYDSFTYNLVQYFGELGEDVRTYRNDEITLDEIARLNP
                     ERICLSPGPSNPQHAGITLDVLREFGGKTPILGVCLGHQAIGEAFGGRVVRAQTIMHG
                     KVSTIETDCKGVFADLPKHFVVTRYHSLAIERESLPDCLEVSAWTEDGEIMGVRHKEL
                     AVEGVQFHPESILSEHGHALLENFVKQSKTAHRNA"
     gene            2370..3401
                     /gene="trpD"
                     /locus_tag="VHV99_18460"
     CDS             2370..3401
                     /gene="trpD"
                     /locus_tag="VHV99_18460"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006406908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX3637427.1"
                     /translation="MSITPQEALQRTIEHREIFHDEMLHLMRLIMRGELSPVMSAAII
                     TGLRVKKETIGEITAAATVMREFARHVEVQDNSNFVDIVGTGGDGSQTFNISTATMFV
                     SAAAGAKVAKHGGRGVSSKSGSADVLEALGVNIDLQPEQVAASIAETGMGFMFAPNHH
                     PAMKNIAPVRRELGVRTLFNILGPLTNPAGAPNQLMGVFHGDLVGIQVRVMQRLGAKH
                     VLVVYGMDGMDEVSLGAATQVGELRDGQIHEYEIHPEDFGMQMVSNRTLKVADAAESR
                     LMLLEALDNKPGVAREIVTLNAGAALYSANVAASIADGIQLAREAIASGKARAKVDDL
                     IRFTQQFKQ"
     gene            3420..4205
                     /gene="trpC"
                     /locus_tag="VHV99_18465"
     CDS             3420..4205
                     /gene="trpC"
                     /locus_tag="VHV99_18465"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEX3637428.1"
                     /translation="MSDILDRIIAVKREEVRAAEQSAPLEELRLEASSRDIRDFVGAL
                     RAKHAAGLAAVISEVKKASPSKGVLRENFVPAEIARSYEKHGAACLSVLTDVQFFQGS
                     VAYLEEARAACNLPVLRKDFIVDPYQIVEARAMGADAILLIAAALETSQMQDLEALAH
                     SLGLAVLVEVHDRNELMEALTLKTPLIGINNRNLRTFETSIETTIGMLESIPDDRIVV
                     TESGILSRGDVDRLRAMAVHTFLVGEAFMRAEEPGVELARMFF"
     gene            4245..>4510
                     /locus_tag="VHV99_18470"
     CDS             4245..>4510
                     /locus_tag="VHV99_18470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEX3637429.1"
                     /translation="MGMEREIKLALPVSQVKAATQWFVARTGNQGRPIELANIYFDTP
                     KLALASSRSALRLRHTPDGWLQTFKTVGNAKDGLHSRHEWEMPVA"
BASE COUNT          927 a         1401 c         1394 g          788 t
ORIGIN      
        1 gtcttcggca accgtttttt acttcgctgc gctcacgcgt tttttccatc accttaagtt
       61 tcgctgcatc ggttgattcg gccaacgtgt acccacaaag tacatctgtg cgctgaaatc
      121 cccttgcatg cgtcgatggg aaactcgcgc cggtcggtat catcgtgtcg aaacgtcgaa
      181 cgaacgcctg accgggcgcg ccgcgccccg ctttgtccga cgcattgtgc cgtctgacgt
      241 acgtacagga tcggaacatg accgaactcg aattccagtc cctcgccaac gagggtttca
      301 accgcattcc gctgatcgcc gaagcgctcg ccgacctcga aacgccactc tcgctgtacc
      361 tgaaactcgc tcagcctgag cgcaacggcg ccaactcgtt cctgctggaa tcggtggtgg
      421 gcggcgaacg cttcggccgc tattcgttca tcggcttgcc ggcgcgcacc ctgctgcgca
      481 cccgcaacgg cgtgtcggaa gtgctgcggg acggcaaagt cgtcgaaacg cacgagggcg
      541 atccgctcga gttcatccag caattccagg ggcgcttcaa agtggcgcag cgtcccgggc
      601 tgccgcgctt cgcgggcggg ttggccggct acttcggtta cgacgcggtg cgctacatcg
      661 agaaaaagct cgctcacacc gcgccgaaag acgatctgaa tctgccggac atccagttgc
      721 tgctcaccga agaagtcgcg gtgatcgaca acctcgcggg caagctctac ctggtggtgt
      781 acgcggatcc gacgcaaccc gaggcgtaca caaaagcccg gcagcgtctg cgtgaattgc
      841 gccagcgttt gcgcacgacc gtgcagccgc cggtcacgtc ggcaagcgtg cgcaccgaga
      901 cctatcgcga attcgcgaaa gacgattatc tggccgccgt gcgcaaggcg aaggaataca
      961 tcgccgctgg cgagttgatg caggtgcagg tcggccagcg tctgatcaag ccttaccgcg
     1021 acaatccgct gtcgctgtat cgcgcgctac gctcgttgaa tccgtcgccg tacatgtact
     1081 actacaactt cggcgacttc cacgtggtcg gcgcgtcgcc ggaaattctg gtgcgccagg
     1141 aaaagcgcgg cgaggatcgc atcgtgacga tccggcctct cgccggcacg cggccgcgcg
     1201 gcaatacgcc ggaacgcgac gcggaactcg ccaccgaatt gctgaacgac ccgaaggaaa
     1261 tcgccgaaca cgtcatgctg atcgacctcg cccgcaacga cgtgggccgc atcgcccaga
     1321 tcggctcggt ggtcgtcacc gacaagatgg tgatcgagaa gtactcgcat gtgcagcaca
     1381 tcgtgagttc cgtcgaaggc aagctgaaac cgggcaccac caatttcgac gtgctgcgcg
     1441 ccaccttccc ggccggcaca ctgtccggtg cgccgaaagt ccgcgcaatg gaactgatcg
     1501 acgaacttga gccagtcaag cgcggcctct acggcggcgc tgtcggctat ctctcgttca
     1561 ccggcgaaat ggatctggcc atcacaatcc gcaccggcgt gatcgcaaac ggcaatctgt
     1621 atgtgcaggc ggcagcgggt gtagtcgcgg attcggtgcc ggaatccgaa tggcaagaga
     1681 ccgagaacaa ggcgcgcgct gtgctgcgcg ccgccgagca ggttcaagac ggcctcgata
     1741 gcgacttctg accggagaca caccatgctg ctcatgatcg acaactacga ctcgttcacc
     1801 tacaacctgg tgcagtactt cggcgaactc ggcgaagacg tgcgcaccta tcggaacgac
     1861 gaaatcacgc tggacgagat tgcgaggctc aacccggagc gcatttgtct gtcgcccggc
     1921 ccgagcaatc cgcaacacgc cggcatcacg ctcgacgtgt tacgcgagtt cggcggcaag
     1981 acgccgattc tcggcgtctg cctcggccat caggcaattg gcgaagcatt cggcggccgc
     2041 gtcgtgcgcg cgcaaaccat catgcacggc aaggtgagca cgatcgaaac cgactgcaaa
     2101 ggcgtgttcg ccgacctgcc gaagcatttc gtcgtaaccc gctaccactc gcttgcaatc
     2161 gaacgcgaat cgctgcccga ctgcctcgaa gtgtccgcat ggaccgagga cggtgaaatc
     2221 atgggcgtgc gtcacaagga actggcggtt gaaggcgtgc agttccatcc ggaatcgatc
     2281 ctgtccgaac acggccacgc attgctcgaa aacttcgtga agcagtcgaa gacggcccac
     2341 cgcaacgctt gatgaagaga gacgaaatca tgtcgattac gccccaggaa gcgctgcaac
     2401 gcacgatcga gcaccgcgaa attttccacg acgaaatgct gcatctgatg cggctcatca
     2461 tgcgcggcga actgtcgccg gtgatgtcgg cggcgatcat caccggcctg cgcgtcaaaa
     2521 aagagaccat cggcgaaatc accgcggccg ccacggtaat gcgtgaattt gcccggcacg
     2581 tcgaggtgca ggacaactcg aacttcgtcg atatcgtcgg caccggcggc gacggctcgc
     2641 aaaccttcaa catttccact gcaaccatgt tcgtttcggc cgcggcgggc gccaaggtcg
     2701 cgaagcatgg cggccgcggc gtgtcgagca agtcgggcag cgcggacgtg ctcgaagcgc
     2761 tcggcgtaaa tatcgatctg cagccggaac aggtggcggc ctcgattgcg gaaaccggca
     2821 tgggcttcat gttcgcgccg aaccatcacc cggcaatgaa gaacatcgcg ccggtgcgcc
     2881 gcgaactcgg cgtgcgcacc ctcttcaata ttctcgggcc gctcaccaat ccggccggcg
     2941 caccgaatca gctgatgggc gtgttccacg gcgatctggt cggcattcag gtgcgcgtga
     3001 tgcagcgcct cggtgccaag cacgtgctgg tggtgtacgg tatggacggc atggacgagg
     3061 tctcgctcgg cgcggccacg caagtcggcg agttgcgcga cggccagatc cacgagtatg
     3121 agatccatcc ggaagacttc ggcatgcaga tggtgtcgaa ccgcacgctg aaagtggccg
     3181 acgccgccga atcgagactg atgctgctcg aagcgctcga caacaagccg ggcgtcgcgc
     3241 gtgagatcgt cacgctgaac gcgggcgcgg ccctctattc ggcgaacgtg gcggcgtcga
     3301 tcgcggacgg cattcagttg gcacgtgaag cgatcgcgag cggcaaagcg cgcgcgaagg
     3361 tcgacgacct gatccgcttc acccagcaat tcaagcagta actacgcagc gagacaccta
     3421 tgagcgacat cctcgaccgc atcatcgcgg tcaaacgcga agaagtccgc gcggccgaac
     3481 aaagtgcgcc gctcgaagaa ctgcgcctcg aagcatcgtc gcgcgacatt cgcgatttcg
     3541 tcggcgcatt gcgcgcgaaa cacgcggcgg gtctggccgc ggtgatttcc gaagtgaaga
     3601 aggcgagccc gtcgaaaggc gtgctgcgcg aaaacttcgt ccctgcggag atcgcgcgtt
     3661 cgtatgaaaa gcacggtgca gcctgcttgt ctgtcctcac cgacgtgcag ttctttcagg
     3721 gcagcgttgc gtatctggaa gaagcgcgcg ccgcctgcaa cctgccggtg ctgcgcaaag
     3781 acttcatcgt cgatccgtat cagatcgtcg aagcccgcgc gatgggcgcc gacgcgatcc
     3841 tgctgatcgc cgccgctttg gaaacctcgc agatgcagga cctcgaagcg ctcgcgcatt
     3901 cgctgggtct cgcggtgctg gtcgaagtgc acgatcgcaa tgagctgatg gaagcgctca
     3961 cgctgaagac gccgctgatc gggatcaaca accgcaatct gcgcaccttc gaaacgtcca
     4021 tcgagacgac catcggcatg ctcgaatcga ttccggacga ccgtatcgtc gtgacggagt
     4081 cgggcattct gtcgcgcggg gatgtggacc gtttgcgggc gatggccgtg catacgttcc
     4141 tcgtcggcga ggcgttcatg cgcgcggaag agccgggtgt ggaacttgcc cgaatgtttt
     4201 tctgagcgca gtttcaacac gcaggctagg tagcggagca agtcatgggc atggaacgtg
     4261 aaatcaagct ggcactgccg gtgagccagg tgaaagcggc gacacagtgg ttcgtcgcgc
     4321 gcaccggcaa ccaggggcgc ccgatcgagc tggcgaatat ttacttcgac acacccaaac
     4381 tggcattggc gagttcaagg agcgcactgc gtttgcggca tacgccggac ggctggctgc
     4441 aaacgttcaa gacggtgggc aacgccaagg acggtctgca cagccggcac gaatgggaaa
     4501 tgccggtggc
//
LOCUS       DATASH010000695         2195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3078711,
            whole genome shotgun sequence.
ACCESSION   DATASH010000695 DATASH010000000
VERSION     DATASH010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3078711"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /gene="murC"
                     /locus_tag="VHV99_18475"
     CDS             <1..230
                     /gene="murC"
                     /locus_tag="VHV99_18475"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003808.1"
                     /note="Catalyzes the formation of
                     UDP-N-acetylmuramoyl-L-alanine from UDP-N-acetylmuramate
                     and L-alanine in peptidoglycan synthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEX3637430.1"
                     /translation="TEVYSAGEAPIVAADGRALARALRVAGKVEPVFVDTVDEVPDAL
                     SAVVRDGDVVITMGAGSIGGVPGRLAQETKV"
     gene            236..1177
                     /locus_tag="VHV99_18480"
     CDS             236..1177
                     /locus_tag="VHV99_18480"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEX3637431.1"
                     /translation="MSGIDPKQFGKVAVLLGGDSAEREVSLNSGRLVLQGLRDAGIDA
                     HPFDPAERPLAALKDEGFVRAFNALHGGYGENGQIQGALDFYGIRYTGSGVLGSALGL
                     DKFRTKLVWQQLGIPTPPFEAVLGGDDYEARAQDIVAKLGLPLFVKPASEGSSVAVIK
                     VKSADALPAALLEAVKYDRIVVVEKSIEGGGEYTACIAGDLDLPVIRIVPAGEFYDYH
                     AKYIANDTQYLIPCGLTPDEEARLKVLARRAFDVLGCTDWGRADFMLDAHGNPYFLEV
                     NTAPGMTDHSLPPKAARAVGISYQELVVGVLALTLKD"
     gene            1202..1954
                     /locus_tag="VHV99_18485"
     CDS             1202..1954
                     /locus_tag="VHV99_18485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004522020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEX3637432.1"
                     /translation="MWNNVRQLNLAANALHALLVLVLLAAGGYWLIQRPNFALREIQI
                     DGDTEHINSPTVRAGVVGRLKGNFFTVDLDVARQAFEQMPWVRHASVRRVWPNALAVT
                     LEEYKPLGTWGSDQLVSVDGELFTANQGELDDDLPAFDGPDGTAKEVVARYSDFKKWF
                     APLGATPDEVTLSPRYAWTVKLSNGMQVELGRERNQDTLLDRSKRLTAAWNAVTQRWG
                     KDIEYADLRYPNGFAIRAAGMRFITEPAKGKK"
     gene            1982..>2195
                     /locus_tag="VHV99_18490"
     CDS             1982..>2195
                     /locus_tag="VHV99_18490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014898179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEX3637433.1"
                     /translation="MSKDYKDLLVALDIGTSKVVAIVAELKGEGHYEVIGLGQSESKG
                     LKKGVVVNIEATVQSIQRALEEAELMA"
BASE COUNT          424 a          618 c          767 g          386 t
ORIGIN      
        1 tgaccgaagt ctattcggcc ggcgaagcgc cgatcgtcgc cgcggacggc cgcgcgctcg
       61 cgcgcgcgct gcgggtggcg ggcaaggtcg agccggtgtt tgtcgatacg gtggatgaag
      121 tgccggacgc gctctcagca gtggtgcgcg acggcgatgt ggtgatcacg atgggcgcgg
      181 gttcgatcgg cggtgtgccg ggtcgcctcg cgcaagagac gaaggtgtga gtgagatgag
      241 cggtatcgat ccgaaacaat tcggcaaagt ggcagtgctg ctcggtgggg attccgccga
      301 gcgcgaggtg tcgctcaatt ccggccgcct cgtgttgcaa ggtttgcgcg acgccggtat
      361 cgacgcgcat ccgttcgatc cggccgagcg cccgctcgcc gcgttgaagg atgaaggctt
      421 cgtgcgcgcg ttcaacgcgt tgcacggcgg ttatggcgag aacggccaga tccagggcgc
      481 gctcgatttc tacggcattc gctacacggg tagcggcgtg ctcggctcgg cgctcgggct
      541 ggacaagttc cgcaccaagc tggtgtggca gcaactcggc attccgacgc cgccgttcga
      601 agcggtgctg ggcggcgacg actacgaagc gcgcgcgcaa gacatcgtcg cgaagcttgg
      661 gcttccgctg ttcgtcaagc cggccagcga aggctcgagc gtcgcggtga tcaaggtgaa
      721 aagcgccgac gcactgccgg cagcgctcct cgaagcggtc aagtacgaca ggatcgtcgt
      781 ggtggaaaag agcattgaag gcggcggcga gtacaccgcg tgcattgccg gcgatctgga
      841 cttgccggtg atccgcatcg tgccggccgg cgagttctac gactaccacg cgaagtacat
      901 cgcgaacgac acgcagtacc tgattccgtg cggtctgacg ccggacgagg aagcgcgtct
      961 gaaagtgctg gcgcgccgtg cgttcgacgt actcggctgc accgattggg gccgcgccga
     1021 tttcatgctg gatgcgcacg gcaacccgta cttcctggaa gtgaacacgg cacctggcat
     1081 gaccgatcat tcgttgccgc cgaaagcggc gcgcgcggtg ggcatcagtt atcaggaact
     1141 ggttgtcggc gtgttggcgc tgacgctgaa ggactaaccg ggacaacccg aaagcaacac
     1201 catgtggaac aacgttcgcc agctcaatct cgccgccaac gcattgcatg cgttgctggt
     1261 gctcgtgctg ctggcggcag gtggttactg gttgattcag cggccgaatt ttgctctgcg
     1321 cgaaatccag atcgatggcg acaccgagca catcaattcg ccaacggtgc gcgccggggt
     1381 agtcgggcgc ttgaagggca actttttcac ggtggatctc gatgtcgcgc gtcaggcatt
     1441 cgagcagatg ccgtgggtgc gtcacgcgag cgtgcggcgg gtctggccga acgcgctggc
     1501 tgtcacgctt gaagagtaca aaccgctagg aacgtggggc agcgatcagc tggtgagcgt
     1561 cgacggcgag ctgttcaccg cgaatcaggg cgagctcgac gatgatctgc ccgcgttcga
     1621 cggcccggac ggcacggcaa aagaagtcgt cgcgcgttat agcgacttca agaagtggtt
     1681 tgcgccgttg ggcgcgacgc cggacgaagt cacgctgtca ccgcgctacg catggacggt
     1741 gaagctctcg aacggcatgc aggtggaact tgggcgcgaa cgcaatcagg acacgctgct
     1801 ggatcgcagc aagcgcctga ccgcggcgtg gaacgcggtg acgcaacgtt ggggaaagga
     1861 tatcgagtat gcggacttgc gctatccgaa cggtttcgcg attcgcgcgg cagggatgcg
     1921 ctttatcacc gaacccgcca agggcaagaa gtaaacggac atcacacgca atgagcacgc
     1981 tatgagtaaa gactataaag atctgctggt tgccctcgac atcgggacgt cgaaggttgt
     2041 ggccatcgtc gccgagttga agggcgaggg tcactacgag gtgatcggcc tcggccagag
     2101 cgaatcgaag gggctcaaga aaggcgtggt ggtgaacatc gaagccaccg tgcaatccat
     2161 tcagcgcgcg ctcgaggaag ccgagctgat ggccg
//
LOCUS       DATASH010000696         1625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5849726,
            whole genome shotgun sequence.
ACCESSION   DATASH010000696 DATASH010000000
VERSION     DATASH010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5849726"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1280
                     /gene="glgB"
                     /locus_tag="VHV99_18495"
     CDS             <1..1280
                     /gene="glgB"
                     /locus_tag="VHV99_18495"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HEX3637434.1"
                     /translation="PLGQFAPSARFGKPEQFATFVDKAHEAGVGVILDWVPAHFPNDA
                     HGLIDFDGTPLYEHADPREGYHQDWNTMIYNLGRNEVSAFLVASGLAWLKRYHVDGLR
                     VDAVASMLYRDYSRAADEWVPNIYGGRENLESIAFLKRLNHEVGYVPGVPGAITIAEE
                     STAWPGVTARVEDGGLGFQFKWNMGWMHDTLHYMEEDPVYRQYHHHMMTFGMVYAYSE
                     RFVLPLSHDEVVHGKGSLLGKMPGDRWQKFANLRAYFGFMWTHPGKKLLFMGGEFGQM
                     AEFNHDTSPHWHLLDDASHHGVQKLVRVLNHLYSAEPALHRLDSEPGGFEWLVGDDSG
                     NSVYAYRRTDGEGGEVVVVCNMTPVPRVGYRIGMPRGGRWVEVLNTDAGVYGGSNMGN
                     SGMIHTEEISSHGRPHAAALTLPPLATIVLRAD"
     gene            1380..>1625
                     /locus_tag="VHV99_18500"
     CDS             1380..>1625
                     /locus_tag="VHV99_18500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme GlgX"
                     /protein_id="HEX3637435.1"
                     /translation="MSHAMPDRLLPGSPYPLGASWDGLGVNFAVFSANAQKIELCLFD
                     PTGRKEIRRFTLPECTDEVWHGYLPNAHPGTAYGFRAH"
BASE COUNT          309 a          496 c          527 g          293 t
ORIGIN      
        1 agccgttggg tcaattcgcg ccctcggcgc gcttcggcaa gcccgagcag ttcgccacgt
       61 tcgtcgacaa ggcgcacgag gccggtgtcg gcgtgattct cgactgggtg ccggcgcact
      121 ttccgaacga cgcgcatggt ctgatcgact tcgacggcac accgctctac gagcacgccg
      181 atccacgcga aggctatcac caggactgga acacgatgat ctacaacctc ggccgcaacg
      241 aggtgagtgc gttcctggtg gcatcgggcc ttgcgtggtt gaagcgctat cacgtcgacg
      301 gcctgcgcgt ggatgcagtc gcctcgatgc tgtatcgcga ctattcgcgc gccgccgacg
      361 agtgggtgcc gaacatttac ggcggccgcg aaaatctcga atcgatcgcg tttctgaagc
      421 ggctgaatca cgaagtcggc tatgtgccgg gggtgcctgg cgcgatcacg atcgccgagg
      481 aatcgaccgc gtggccgggc gtgacggcgc gcgtcgaaga cggcggcctc ggcttccagt
      541 tcaagtggaa catgggctgg atgcacgaca cgctgcacta catggaagag gatccggtct
      601 atcgccaata ccatcatcac atgatgacat tcgggatggt gtatgcgtat tccgagcgct
      661 tcgtgctgcc gctctctcac gacgaggtcg tgcacggcaa aggctcgctg ctcggcaaga
      721 tgccgggcga cagatggcag aagttcgcca acctgcgcgc gtacttcggc ttcatgtgga
      781 cgcatccggg caagaagctg ctgttcatgg gcggtgaatt cggtcagatg gcggaattca
      841 atcacgacac ctcgccgcac tggcatctgc tcgacgatgc gagccatcac ggcgtccaaa
      901 agctggtgcg cgtcctgaac catctgtaca gcgcggagcc tgcgctgcac cgactcgaca
      961 gcgagcctgg cggcttcgaa tggctggtcg gcgacgacag tggcaatagc gtgtacgcct
     1021 atcgccgcac cgacggcgaa ggcggcgaag tcgtggtggt gtgcaacatg acgccggtgc
     1081 cgcgagtcgg ctaccggatc ggcatgccgc gcggcggccg ctgggtcgaa gtgctgaata
     1141 cggatgccgg cgtgtacggc ggctcgaaca tgggcaacag cggcatgatt cacactgagg
     1201 aaatctcgag ccacggcagg ccgcatgccg ccgcgctgac gctgccgccg ttggcgacga
     1261 tcgtcctgcg cgcggattaa cggagctgat gtaagaacgg gcgcgcagcc tcgcggccgc
     1321 gcgcccgttg catgaagacc cacgatagcg ttgataagac agaacaagga aggggaatca
     1381 tgtcgcatgc aatgcccgac cggcttctgc ccggctcgcc ctatccgctt ggcgccagtt
     1441 gggatggcct tggcgtcaac tttgcagtgt tttcggcgaa tgcacagaaa atcgagcttt
     1501 gcctgttcga tcccacgggt cgcaaggaaa tccggcgctt cacgctgccc gaatgcaccg
     1561 acgaagtctg gcacggttac ctgcccaacg cgcaccccgg cacggcttac ggctttcgcg
     1621 cacat
//
LOCUS       DATASH010000697         4579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3673094,
            whole genome shotgun sequence.
ACCESSION   DATASH010000697 DATASH010000000
VERSION     DATASH010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4579
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3673094"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VHV99_18505"
     CDS             <1..592
                     /locus_tag="VHV99_18505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HEX3637436.1"
                     /translation="RVYGQHAAIDWRPHTEVVVNREFSLGSVLSVHTAAEALTRGGDV
                     LLMDADVLYDERILEPLVAGAGPVNRLLIDRDFEAGDEPVKLCVANGVPVELRKQVLA
                     DLQFDVIGESVGFFRFDETAARRLAELVAGYVATGRANLPHEEAVRDLLLERSQRFDV
                     ADVTGAPWIEIDFQNDVKRAADEVLPQLRQLAGALR"
     gene            589..2289
                     /gene="aepX"
                     /locus_tag="VHV99_18510"
     CDS             589..2289
                     /gene="aepX"
                     /locus_tag="VHV99_18510"
                     /EC_number="5.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050188 - phosphoenolpyruvate mutase
                     activity [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate mutase"
                     /protein_id="HEX3637437.1"
                     /translation="MNAPYQLPAAYARSLRLREMLQSNQLEFLMEAHNGLSARIVKEA
                     GFKAIWGSGLTISAQFGVRDNNEASWTQVVDTLEFMADASDLPILLDGDTGYGNFNNV
                     RRLVKKLEQRGVAGVCIEDKQFPKTNSFLNGERQPLAEMDEFCGKIKAGKDSQSDPNF
                     SIVARVEALIAGWGMEEALKRAEAYRQAGADAILIHSKLSKPDEILTFAREWAGRGPL
                     VIVPTKYYSTPTDVFRQAGISTVIWANHLLRGAASTMQAIAKEIHSSETLVNVEDRVA
                     TVNEIFRLQDAEEYSTAESVYLSSASASRNAVVLAASRGAGLEAVTADKPKVMLPIAG
                     KPLLRRLVEAFKKEGINDITVVGGYRADAIDTSGIRLVVNEKHEQTGELASLACAAKS
                     FTADTVISYGDLLFRSYILRDLVESDSDFCVVVDSSQTPAAGVASTDFAYCSTADDRA
                     LFGQKVWLDSVTSSVGPANSKTPQGRWMGLLNVRGAGRERLVAMLEKLQQRDDFDKLD
                     MAALLNALVAADEKIEVQYVHGHWRGVNDLDELRQAGDFAHGQAPYGSNGSGSSEAGA
                     "
     gene            2286..3476
                     /gene="aepY"
                     /locus_tag="VHV99_18515"
     CDS             2286..3476
                     /gene="aepY"
                     /locus_tag="VHV99_18515"
                     /EC_number="4.1.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017329755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033980 - phosphonopyruvate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonopyruvate decarboxylase"
                     /protein_id="HEX3637438.1"
                     /translation="MIEAAQFVEAARERGFDWYAGVPCSYLTPFINYVLQDESLNYVS
                     AANEGDAVALIAGVELGASGVHKARRGISMMQNSGLGNAVSPLTSLTWTFRLPQLLIV
                     TWRGQPGVADEPQHALMGPVTPAMLDTMEIPWELFPTEAEAIGPALDRATVHMDSTGR
                     PYALVMQKGSVAPYKLNKKGLSGVRQRALNERAEVQLFKSAGAGDRVSGVSRHDALQR
                     VIAHTPKESTVVLASTGFCGRELYAIDDRENQVYLVGSMGCVTPMALGLALSRPDLNV
                     VALDGDGAALMRMGVFATLGAYGPSNLTHLLLDNGAHESTGGQATVSQGVEFARIASA
                     CGYALALDGDDLSVIDRLFDAKDIDGARFARLSINTGTPSDLPRPSITPEDVRRRLQT
                     HIGR"
     gene            complement(3573..4043)
                     /locus_tag="VHV99_18520"
     CDS             complement(3573..4043)
                     /locus_tag="VHV99_18520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008350894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637439.1"
                     /translation="MKPFAQAVRIGLFILIGASSAQASAYALDAQLDCKSTAHAFIAP
                     LQENHAIETVPMRVEPNSINAFKPTRGSHLTAFDFGVFAVVGFQKNDAIFKEGSGEPI
                     ADSAYGAVVFGGEDAVRQKVRAAGSDAVIRHVAPFITAIFCKPDSPQLATVTHG"
BASE COUNT          905 a         1317 c         1502 g          855 t
ORIGIN      
        1 ccgtgtgtac ggacagcacg ctgcgatcga ctggagaccg catacggaag tggtcgtgaa
       61 tcgcgagttt tcgctcggca gcgtgctgtc cgtacacacg gcggccgaag cactgacccg
      121 cggcggcgat gtgctgctga tggacgccga cgtgctttac gacgaacgta ttctcgagcc
      181 gctggtggcg ggcgcggggc cggtgaaccg gttgttgatc gaccgtgatt tcgaagccgg
      241 cgatgaaccc gtgaaacttt gtgtcgcgaa tggcgtgccg gtggagttgc gcaagcaggt
      301 gctggccgat ttgcaattcg acgtgatcgg cgagtcggta ggatttttcc gcttcgacga
      361 aaccgcggcg cggcgtctgg ccgaactcgt ggcgggatat gttgcgacgg gccgcgccaa
      421 cttgccgcat gaagaagccg tacgcgattt gctgctggaa cgcagccagc gattcgacgt
      481 ggccgatgta accggcgcgc catggataga aatcgatttt cagaacgacg ttaaacgcgc
      541 ggccgacgaa gtcttgccgc aactgcgtca gcttgcagga gccctacgat gaacgcaccg
      601 tatcaactac ccgctgccta tgcccgcagc cttcgccttc gcgagatgct gcaaagcaat
      661 cagctcgaat tcctgatgga agcgcacaac ggcttgtccg cacgcattgt gaaggaagcc
      721 ggctttaagg caatttgggg atcgggtctc acgatctccg cgcagttcgg cgtacgcgac
      781 aacaacgaag cgagctggac gcaagtggtc gacacgctgg agttcatggc ggacgcgagc
      841 gacctgccca tcctgctcga tggcgacacc ggttatggca acttcaataa cgtccgccgg
      901 ttggtgaaga agctggagca gcgcggcgtt gccggcgtat gtattgaaga caagcagttt
      961 ccgaagacca acagttttct caacggcgag cgtcagccgc tcgccgagat ggacgagttc
     1021 tgcggcaaga tcaaggccgg caaggactcg caaagcgatc cgaatttctc catcgtcgcg
     1081 cgcgtggaag cgttgatcgc gggctggggc atggaagaag cgttgaagcg cgcggaggct
     1141 tatcgccagg ccggcgcgga cgccatcctg attcacagca agctttccaa gccggacgaa
     1201 atcctgacgt ttgcacgtga atgggccggc cgcggaccgc tcgtgatcgt gccgaccaag
     1261 tactacagca cgccgaccga tgtgtttcgc caggccggca tcagcacggt gatctgggcg
     1321 aaccatttgc tgcgcggcgc ggcgtcgacc atgcaggcga tcgcgaagga aatccacagc
     1381 agcgagacgc tcgtgaacgt ggaagaccgc gtggcgacgg tgaacgaaat cttccgtctc
     1441 caggacgccg aggaatattc gacggctgaa agcgtgtatc tgtcgtcggc aagtgcatcg
     1501 cgcaacgctg tcgtgctcgc ggcaagccgg ggcgcggggc tggaagccgt taccgccgat
     1561 aaacccaagg tgatgctgcc gatcgcgggc aagccgctct tgcgccgtct ggtcgaagcg
     1621 ttcaagaaag aaggcatcaa cgacattacg gtggtcggcg gttatcgcgc cgatgccatc
     1681 gatacatcgg ggattcggct ggtcgtgaac gagaagcacg agcaaacggg cgaactggcg
     1741 tcgctggcgt gcgccgcgaa atcgttcacc gccgacaccg tgatctccta cggcgacctc
     1801 ttgttccgca gctacattct tcgcgatctg gttgagtcgg acagcgattt ctgcgtggtc
     1861 gttgattcgt cgcagacgcc ggcagccggg gtggcatcga cggatttcgc gtattgctcc
     1921 acagcggacg atcgtgcgtt gtttggtcag aaggtctggc tcgacagcgt gacgagctcg
     1981 gtgggaccgg caaattcgaa gacgccgcaa ggccgctgga tgggtctgct gaacgtgcgc
     2041 ggcgcagggc gtgaacgcct cgtcgccatg ctcgaaaagc tgcagcaacg cgatgacttc
     2101 gacaagctcg acatggccgc gttgctcaac gcgctggtcg ccgcggacga aaagatcgaa
     2161 gtgcagtacg tgcacggtca ctggcgcggc gtgaacgacc tcgacgagtt gcgtcaagcc
     2221 ggagatttcg ctcacggaca agcgccgtac ggatcgaacg gctcgggctc ctcggaggct
     2281 ggcgcgtgat cgaagccgca caatttgtcg aagctgcgcg cgagcggggc ttcgattggt
     2341 acgcgggcgt gccgtgctca tatctgacgc cgttcatcaa ctatgtgctg caggacgagt
     2401 cgctgaacta tgtgtcggcg gccaacgagg gtgacgctgt tgcgttgatc gcgggcgtgg
     2461 agctgggcgc atcgggtgtg cataaggcgc gtcgcggcat ttcgatgatg cagaactccg
     2521 ggctcggcaa cgcggtgagc ccgctcacct cgctcacgtg gaccttcagg ctgccgcaat
     2581 tgctgatcgt cacgtggcgc ggtcagccgg gcgtcgccga tgagcctcag cacgcgctga
     2641 tgggacccgt caccccagcc atgctcgaca ccatggagat tccatgggag ctgttcccga
     2701 ctgaagccga ggcgatcgga cccgcgctcg atcgcgcgac cgtgcatatg gacagcaccg
     2761 ggcgcccgta cgcgctagtg atgcagaagg gcagcgttgc gccatacaag ttaaacaaga
     2821 aaggcttgag cggtgtgcgt cagcgcgcgt tgaacgagcg cgccgaggtt cagttgttca
     2881 aaagcgcagg cgctggcgat cgcgtgtcag gtgtgtcacg ccacgacgcg ttgcagcgcg
     2941 tgattgcaca cacgccgaaa gagtcgacgg tcgtgttggc atcgacggga ttttgcgggc
     3001 gggagctcta cgctatcgat gaccgcgaga accaggtgta cctggttgga tcaatgggat
     3061 gcgtgacgcc gatggcgctc ggcctcgcgt tgtcgcggcc tgacctgaac gtggtagcgc
     3121 tcgacggcga cggcgctgcg ctgatgcgca tgggcgtgtt cgcgacgctc ggcgcttacg
     3181 gcccgtccaa cctcacgcat ttgctgctgg acaacggtgc gcatgagtcg actggcggcc
     3241 aggcgacggt gtcgcaaggc gtcgagttcg cgcgaatcgc ttcagcctgc ggatatgcgc
     3301 ttgcgctgga cggcgatgac ctctctgtca tcgaccggct gtttgacgcg aaggatatcg
     3361 acggcgcgcg tttcgcgagg ctgtcgatca acaccggcac gccgagcgac ctgccgcgcc
     3421 cgtcgatcac gccggaagac gtgcgtcgcc ggttgcaaac gcatattgga cgctagaagg
     3481 ccggttgttg ccgaaccccg gtcctgtcac gctgttcacc caaaggccgc accaacccga
     3541 cgttggcgcg gcctttggat agtgggcccg tgttaaccgt gggtgaccgt ggctaattgt
     3601 ggtgaatcgg gcttgcagaa gatcgcggtg ataaaaggcg cgacgtgccg aatcacggcg
     3661 tcgctgccag ccgcgcgaac tttttgcctg accgcatctt ccccgccgaa cacaacggcg
     3721 ccataagccg aatcggcaat aggttcaccg ctgccttcct tgaaaatcgc gtcattcttc
     3781 tggaacccga ccaccgcgaa cacgccgaaa tcgaacgccg tgagatgact accgcgcgtt
     3841 ggtttaaacg cgttgattga attgggctcc acgcgcatgg ggacagtttc tatcgcgtgg
     3901 ttttcctgaa gcggcgcgat gaacgcatgc gcggtcgatt tacagtcgag ttgagcgtcc
     3961 agcgcatagg ccgaagcttg cgcggacgat gctccaatga ggatgaataa gccgatgcgg
     4021 acggcttgtg cgaatggctt catgaacggt gagtacgcgc tcgggcgctg cgaaaagcat
     4081 agactttagc cggaagccgg aaagcgtgct taaacaagtg cttagagccc gtccggcggg
     4141 acgcggcgag gcgtggttcg cgtttggctt acggcgaaat tcggggaaat tcgtcggccg
     4201 gtaatcctca gcagaatggc gatgagctgt gcgaatgggt tcgatgagtg cgcttaaacc
     4261 caccccatcg cccaaggaca agctaagcga aattaaagcc tgcaagcagg cttcaggagt
     4321 gaaggcagaa gatggtgagc atgccgaatt tgctcggcgc gctgaaagtt cgcggtgaat
     4381 ttattaagga ggcgtacggc gcacgagcgg cacgaccgta cgctcaggct cacgtatcac
     4441 cggccagacc gtatggcgaa ccgcaactcc ctcaagccgc tgccaattca ggcaccgttc
     4501 cacgccgcgt ctctctccga tgcacgcatg ttcctgccgc atacgtctcg aacacggcgc
     4561 ggtcgtcgcc gagcatcgc
//
LOCUS       DATASH010000698         3755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1103748,
            whole genome shotgun sequence.
ACCESSION   DATASH010000698 DATASH010000000
VERSION     DATASH010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1103748"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VHV99_18525"
     CDS             complement(<1..440)
                     /locus_tag="VHV99_18525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEX3637440.1"
                     /translation="MPSRLTPSAVAASVVAPAAPALPDFSTRLIAWQRQHGRHDLPWQ
                     NTRDPYRIWLSEIMLQQTQVSTVIPYYARFLARFPDVAALAAAPIDDVMALWAGLGYY
                     SRARNLHRCAQAVVELHGGAFPASVEALADLPGIGRSTAAAIAS"
     gene            complement(446..1195)
                     /gene="mutM"
                     /locus_tag="VHV99_18530"
     CDS             complement(446..1195)
                     /gene="mutM"
                     /locus_tag="VHV99_18530"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEX3637441.1"
                     /translation="MRTPALRWPIPADLAKTLRGSVVRKVERRGKYLLFEIDAGWFIV
                     HLGMTGTLRVLRHVPHPPAAAKHDHVDWIFDEFILRYRDPRRFGAVLWHPREAGDVLE
                     HPLLAGLGVEPFSPVFSGALMHRLTRGRKVSVKQALLAGEIVVGVGNIYASESLFRAG
                     IRPTTAAGRVSLVRYDLLADAVRVTLAAAIEKGGSTLRDFVGSDGESGYFQLDYFVYD
                     RAGLPCRVCGTPIKQIVQGQRSTYFCPTCQR"
     gene            1364..3205
                     /locus_tag="VHV99_18535"
     CDS             1364..3205
                     /locus_tag="VHV99_18535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3637442.1"
                     /translation="MNLSFVKLFSKRPASASRVPHAVSARRLLGAAALAVWALAAVPA
                     HAQDPSPDQDDTSVTLPDPLGPASAEDQKSLPDVPLSSQIVFQVLAAEVALQRDQPAP
                     AYQTYLALARDTHDPRMAQRATEIALAAQSPSDALAAAQLWQQYAPNSERAAQLDASL
                     LVLSGKPDDAAPILARELAKVPADNRGSAMLALQLLLSRGPNRIGGLHVLQDLLKNDL
                     NRPEAQLAIARQQLVSNDAPGARKSLEQALTLKPDYLPAALMLSQMGPEERKEGIASL
                     EKYVQQNPKSHDARLALAQMYLASDRLDDAQKQFEIMHKNNAADLTPLMALALIKIQQ
                     KNFNDAQTYLIQYAQQAEKTPGADPGQAYIYLAQLSLEQKNEAAASDWLNKVTPSSQQ
                     FLPAQITRAQLLAKQGKADDARKLLANLQAPDPRDQALIVRTDAAILFDAKRYAEAET
                     RLQQATAAFPDDPDLTYDYAMAAEKTGHYDVMEAQLRKLIQTQPDNPQAYNALGYSLA
                     DRNQRLPEADQLVEKASSLAPNDAFIMDSVGWVKYRMGDTTDAIKLLRKAYDIQPNAE
                     IGAHLGEVLWKTGAQDQARAAFRDARKLEPDNDTLVKTLQRLQVNDL"
     gene            3205..>3755
                     /gene="lolB"
                     /locus_tag="VHV99_18540"
     CDS             3205..>3755
                     /gene="lolB"
                     /locus_tag="VHV99_18540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018434687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HEX3637443.1"
                     /translation="MRLSRLISYPRAPRGVALGFVAAAVVALAGCASVKPQGPSTSNA
                     ATAVTAQTTRAYQGRFAIQYNDQNGQQRNAYGNFTWQETGDTVTLQLRNPLGQTLAIV
                     TSSSASATLELPNRQPLTADNVSTLMQNALGFVLPVEGLRYWLQPSPAPTSRAKTEKD
                     PEQPSRLKQITQDGWTIDYLAYA"
BASE COUNT          766 a         1253 c         1147 g          589 t
ORIGIN      
        1 aatgatgcaa ttgccgcagc agtcgagcgc ccgatgcccg gcagatcggc aagcgcttcg
       61 acggacgccg gaaaggcgcc gccatgcagt tcgaccaccg cctgcgcaca ccgatgcaga
      121 ttgcgcgcgc gcgagtagta cccgagaccg gcccacaggg ccatcacatc gtcgatcggc
      181 gctgcggcca gcgcagcgac atcgggaaag cgcgcgagga accgcgcgta gtacggaatc
      241 accgtcgaca cctgggtttg ctgcagcatg atttccgaca accagatgcg atacgggtca
      301 cgcgtgttct gccagggcag gtcgtggcgg ccgtgctggc gctgccaggc aatcaggcga
      361 gtggaaaaat ccggcagggc gggggcggca ggcgcgacga ctgaggcggc tacggcggac
      421 ggcgtcaagc gagagggcat gagaattagc gctggcaggt aggacaaaag taagtcgagc
      481 gctgcccttg cacgatctgt ttgatcggcg ttccacacac acggcatggc agccccgcgc
      541 gatcatagac gaaatagtcg agctggaagt aaccgctttc gccatcactg ccgacgaaat
      601 cgcgcagcgt gctgccgccc ttctcgatcg ctgcggcgag cgtgacgcgc accgcgtccg
      661 ccagcaaatc gtaacgcacg agcgagaccc ggcccgccgc ggtggtagga cggatcccgg
      721 cgcgaaacag gctttcggag gcgtagatat tgcccacgcc gaccacgatt tcccccgcca
      781 gcaaggcctg tttcaccgac accttgcgac cgcgcgtgag ccggtgcatc aatgcacctg
      841 aaaataccgg cgaaaacggc tcgacaccga ggcccgcgag cagcggatgc tcgagcacgt
      901 cgccggcttc acgcgggtgc cacagcacag cgccaaagcg ccgcggatcg cgatagcgca
      961 aaatgaattc gtcgaagatc cagtcgacgt gatcgtgttt cgccgcggcc ggcggatgcg
     1021 gcacgtggcg cagcacacgc agcgtgccgg tcatgccgag atgcacaatg aaccagccgg
     1081 cgtcgatttc gaacagcagg tacttgcccc gccgctcgac cttacgtacc acgctgccgc
     1141 gcaaggtttt cgcaaggtcc gccggaatcg gccagcgcag cgcgggcgtg cgcacatcga
     1201 cgcgttaaac cttgcggccg gacacatacg gttctattcc ccttcgggta acctcaactt
     1261 ctggcaactc tggcatgtct gactggtctg caacttgcgt gagtggttgt gcgcttattg
     1321 tagcgagcgc gttacaatcg tccgaaacat tgaacggatt tccatgaact tgtccttcgt
     1381 gaagctgttt tcgaagcgcc cggctagcgc atcccgcgtg ccgcacgccg tgtccgctcg
     1441 tcgcctgctc ggcgccgccg cgctcgccgt ctgggcgctg gccgccgtgc ccgcgcacgc
     1501 gcaggaccca tcgccggacc aggatgacac gtccgtcacc ctgccggacc cgctcggccc
     1561 agcgtcggca gaagaccaga agtctctgcc ggacgtgccg ctgtccagcc agatcgtatt
     1621 tcaggtgctc gccgctgaag tcgccctgca acgcgaccag ccggcgcctg cctaccagac
     1681 ctaccttgcg ctcgcgcgcg acacgcacga tccgcgtatg gcgcaacgcg ccacggaaat
     1741 cgcgctggcc gcgcaaagtc cgtcggacgc cttagccgcc gcgcaattgt ggcagcagta
     1801 tgcgccgaat tcggaacgcg ccgcgcaact cgacgcctcg ctgcttgtgc tgtccggcaa
     1861 gcctgacgac gccgcgccga tcctcgcccg cgaactcgca aaagtccccg ccgacaatcg
     1921 cggcagcgcg atgctggcgc tgcagttgct gctgtcgcgc ggcccgaaca ggatcggtgg
     1981 cttgcatgta ttgcaagacc tgctgaagaa cgacctgaac cgcccggaag cgcagttggc
     2041 catcgcacgc cagcagctcg tttcgaacga cgcgccgggc gcgcgcaagt cgctcgaaca
     2101 ggcgctcacg ctcaagcccg actatctgcc ggccgcgctg atgctctcgc agatgggccc
     2161 ggaggagcgc aaggaaggca tcgcgtcgct cgaaaagtac gtgcagcaga atccgaaatc
     2221 gcatgacgca cgtctcgcgc tcgcgcagat gtacctcgcg agcgaccgcc tggacgacgc
     2281 gcagaagcag ttcgagatca tgcacaagaa caacgcggcg gacctcacgc cgctgatggc
     2341 gctcgcgctg atcaagatcc agcagaagaa tttcaacgac gcgcaaacct acctcatcca
     2401 gtacgcgcag caagccgaga aaacgccggg cgccgatccg ggccaggcgt atatctatct
     2461 cgcgcaacta tcgctcgaac agaagaacga agcggcggcc agcgactggc tgaacaaggt
     2521 aacgccttct agccagcaat tcctgccggc gcagattacg cgcgcccaat tgctcgccaa
     2581 gcagggcaag gccgacgacg cgcgcaagct gctggcaaac ttgcaagcgc ccgatccgcg
     2641 cgaccaggcg ctgatcgtgc gcaccgacgc ggccattctg ttcgacgcaa agcgctacgc
     2701 cgaggcggaa acacgcctgc aacaagcaac ggcggctttc cccgacgatc ccgatctcac
     2761 ctacgactac gccatggcgg ccgagaagac cggccattac gatgtcatgg aagcgcaact
     2821 gcgcaagttg atccagacgc agccggacaa cccgcaggct tataacgcgc tcggctattc
     2881 gctggccgat cgtaatcagc gcctgccgga agcggaccag ctggtcgaga aagcctcgtc
     2941 gctggcgccg aacgacgcgt tcatcatgga cagcgtcggc tgggtcaagt accggatggg
     3001 cgacaccacg gatgcaatca agctgctgcg caaagcgtac gacattcagc cgaacgccga
     3061 aatcggcgcg catctcggcg aagtgttgtg gaagaccggc gctcaggatc aggcgcgcgc
     3121 cgctttccgc gacgcgcgca agctcgaacc ggacaacgac acgcttgtca aaacgctcca
     3181 acgtcttcag gtgaacgatc tttgatgcgt ctttcccgct tgatctccta tccccgggcg
     3241 ccgcgtggcg tggcgctcgg attcgtagcg gcagcggttg tcgcgttggc tggttgtgca
     3301 tcggtgaagc cgcaggggcc gtccacatcg aatgcggcga cagccgtcac tgcacaaacc
     3361 acgcgcgctt accaaggtcg tttcgcgatc cagtacaacg atcagaacgg gcagcagcgc
     3421 aatgcctatg gcaacttcac atggcaggaa acgggtgata ccgtcacgct gcaattgcgc
     3481 aatccgctcg gccagacact ggcgatcgtg acttcgtcat cggcttcggc cacgctcgaa
     3541 ttgccgaacc gtcagccgct caccgccgac aatgtctcca cgctgatgca gaacgcgctt
     3601 ggcttcgtgc tgccggtcga ggggttgcgt tactggctgc agccgtcgcc ggcgcccact
     3661 tcgcgcgcca aaaccgagaa ggatccggag caaccctccc gcctgaaaca gatcacgcag
     3721 gacggctgga cgatcgatta cctggcctat gccga
//
LOCUS       DATASH010000699         2324 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3673604,
            whole genome shotgun sequence.
ACCESSION   DATASH010000699 DATASH010000000
VERSION     DATASH010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3673604"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..153)
                     /locus_tag="VHV99_18545"
     CDS             complement(<1..153)
                     /locus_tag="VHV99_18545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011851752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX3637444.1"
                     /translation="MAKIGFIGLGIMGAHMARNLIKGGHTLFVNGAYPVPEDLSKSAS
                     VVANSTA"
     gene            complement(261..1070)
                     /gene="hyi"
                     /locus_tag="VHV99_18550"
     CDS             complement(261..1070)
                     /gene="hyi"
                     /locus_tag="VHV99_18550"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011352319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HEX3637445.1"
                     /translation="MPKFAANLTMLFNEVPFLDRFAAAADAGFNAVEFLFPYPYQIAE
                     LSERLQQNRLKLVLHNLPAGNWEAGERGIACLPDRVGEFQEGVGRAIEYAKALKVPQL
                     NCLVGIPTAGVDADKARSTIVDNLRFAAGELKKAGIKLLVEPCNSYDIPGFALNRSSE
                     GLDVIRAVGSDNLFLQYDIYHMQRMEGELAATIKKNLPEIAHIQLADNPGRNEPGTGE
                     INYPFLFDLLDSLGYAGYVGCEYKPRTTTAAGLVWVQSVAGQTRGAARAAA"
     gene            complement(1212..>2324)
                     /gene="gcl"
                     /locus_tag="VHV99_18555"
     CDS             complement(1212..>2324)
                     /gene="gcl"
                     /locus_tag="VHV99_18555"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014896848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HEX3637446.1"
                     /translation="LLVEFAETIGVPVIPTLMSWGAIPDDHPLMAGMVGLQTSHRYGN
                     ATMLASDFVLGIGNRWANRHTGSIEVYTKGRKFVHVDIEPTQIGRVFGPDLGIVSDAK
                     AALELFVEVAQEWKAAGKLKDRSAWVADCQQRKRTMHRKTHFDNVPMKPQRVYEEMNQ
                     VFGRDTCYVSTIGLSQIAGAQFLHVYKARNWINCGQAGPLGWTIPAALGVRAADPQRP
                     IVALSGDYDFQFMIEELAAGAQFKLPYVHVVVNNSYLGLIRQAQRAFDMDFCVQLGFE
                     NINSPETNGYGVDHVAVAEGLGCKAIRVFKPEELKPALLKAQSMLSEFNVPVIVEVIL
                     ERVTNISMGTEIDAINEFEELAEKRADAPTAISMLD"
BASE COUNT          400 a          712 c          721 g          491 t
ORIGIN      
        1 agcggtcgaa ttggcgacga cgctcgcgct cttgctcaga tcttccggca ccgggtacgc
       61 gccattcacg aacagcgtgt ggccgccttt aatgaggttg cgcgccatat gcgcgcccat
      121 gatgccgagg ccgataaaac cgatctttgc catgtgtcta aatctccagt gttgtttgag
      181 atccccaggg ggacgtgctt cggggcgtag tgcgcctgat gtgatccgcg cgtcgcctgg
      241 tgcgtcaccc agtgtgtcaa tcaggccgcg gcgcgggctg cgccgcgggt ttgcccggcc
      301 acgctctgca cccagacgag gccggcggcc gtggtcgtgc gcggtttgta ttcgcaaccg
      361 acatagcctg cgtagccgag cgaatcgagc aggtcgaaca ggaacgggta gttgatttcg
      421 ccggtgcccg gttcgttacg gcccgggttg tcggcgagct ggatgtgcgc gatctccggc
      481 aggttctttt tgatcgttgc cgcgagttcg ccctccatcc gttgcatgtg atagatgtcg
      541 tattgcagga acagattgtc cgaacccact gcgcgaatca cgtcgagacc ttcgctcgaa
      601 cggttcagcg caaagcctgg gatgtcgtac gaattgcacg gctcgaccag cagcttgatg
      661 ccggctttct tcagttcgcc cgcggcgaag cgcaggttgt cgacgatcgt cgagcgagcc
      721 ttatccgcat cgacgcccgc cgtcggaatg ccgacgaggc agttcagttg cggcaccttc
      781 aaggcctttg cgtactcgat cgcgcggcct acgccctcct gaaactcgcc cacgcgatcc
      841 ggcaggcagg cgataccgcg ttcgcccgct tcccagttgc ccgcgggcag gttgtgcaac
      901 accagcttca aacggttctg ctgcagacgt tcgctcaatt ctgcgatctg atacggatac
      961 ggaaacagga attcgacggc gttgaagcct gcgtccgctg ccgccgcaaa gcggtcgagg
     1021 aacgggactt cattgaacag catggtgaga ttcgctgcaa atttcggcat ggtgcttttt
     1081 tctcgctggt cggtcagtgg aataacttca tgaggcggcg cggtgatgct gggccgcgct
     1141 cacgtgatgc ttatcaggtc gacccgcggg tcgacccgca ggtcaacgcg cggatcgaag
     1201 cgtgaggacg ctcagtcgag catgctgatt gccgtcggtg cgtcggcgcg cttctcggcc
     1261 agttcttcga actcgttgat cgcgtcgatc tcggtgccca tcgaaatgtt ggtcacacgt
     1321 tcgaggatca cttcgacgat caccggcacg ttgaactcgg agagcatcga ttgcgctttc
     1381 aacagcgccg gtttcagttc ttccggcttg aacacgcgga tcgccttgca acccaggcct
     1441 tcggcaaccg ccacgtggtc cacgccgtag ccgttcgttt ccggcgaatt gatgttctca
     1501 aagccgagtt gcacgcagaa gtccatgtcg aacgcgcgtt gtgcctggcg gatcaagccg
     1561 aggtacgagt tgttcaccac cacgtgcacg tacggcagct tgaattgcgc gccggctgcc
     1621 agctcttcga tcatgaactg gaagtcgtag tcgcccgaga gcgcgacgat cggacgttgc
     1681 gggtctgctg cacgcacgcc gagcgctgcc ggaatcgtcc agccgagcgg gcctgcctgg
     1741 ccgcagttga tccagttgcg cgccttgtag acgtgcagga attgcgcgcc ggcgatctgc
     1801 gacaagccga tcgtgctgac gtagcacgta tcgcggccga acacctggtt catttcttcg
     1861 tagacgcgct gcggcttcat cggcacgttg tcgaagtgcg tcttgcgatg catggtccgc
     1921 ttgcgctgct ggcaatcggc gacccatgcg ctgcggtcct tcagcttgcc ggcggccttc
     1981 cattcctgcg ccacttcaac gaacagttcg agcgcggctt tcgcgtccga cacgatgccg
     2041 aggtccggac cgaacacgcg accgatttgc gtcggctcga tgtccacatg cacgaacttg
     2101 cggcccttcg tataaacctc gatgctgccc gtgtgacgat tcgcccaacg gttgccgatg
     2161 ccgagcacga agtcggaggc gagcattgtc gcgttgccgt agcggtgcga ggtttgcaag
     2221 ccgaccatgc cggccatcag cggatggtcg tcgggaatcg cgccccacga catcagtgtc
     2281 gggatcaccg gcacgccgat cgtttcggcg aattcgacca gcag
//
LOCUS       DATASH010000700        16391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4467343,
            whole genome shotgun sequence.
ACCESSION   DATASH010000700 DATASH010000000
VERSION     DATASH010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 16391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16391
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4467343"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(273..1084)
                     /locus_tag="VHV99_18560"
     CDS             complement(join(273..745,745..1084))
                     /locus_tag="VHV99_18560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011879774.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEX3637447.1"
                     /translation="MPKELIDNELWSLIEPLLPARAPRNRQYAGRKPTPDRAVLTGIV
                     FVLRSGIAWNLLPQEMGCGSGTACWRRLVAWQEAGVWQRIHETLLAELRRRGEIDLSR
                     ALVDSSSIRAMPGGKKTGPNPTDRRKLGSKHHLIVDAKGIPLAVILSGANRHDVTQLD
                     ALVDAIPHIRGKRGRPLHKPQIVQGDRGYSSEAHRQRLRKRGITPMLAKIGSPHGSGL
                     GKTRWPVERSIAWLHSCLRLKIRYERYAHVHEAFLSLACALICWTRLKPTFN"
     gene            complement(1252..1515)
                     /locus_tag="VHV99_18565"
     CDS             complement(1252..1515)
                     /locus_tag="VHV99_18565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637448.1"
                     /translation="MPWEHIDRWLGAEKFFRAGQRVYVSVDYSRQSLMVTPEYDEAAK
                     ARERAQRTIERKAENDELRGVWLAQEAERQRRRNRSGPNTSNL"
     gene            1600..1854
                     /locus_tag="VHV99_18570"
     CDS             1600..1854
                     /locus_tag="VHV99_18570"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HEX3637449.1"
                     /translation="MHYDRHRYYDPTTERFISKDSIGLAGGIKLYQYANNPALRPSRF
                     HPAMHRVHATKTPHQNLTQKTIRQPISTHRLIRTRKAKSQ"
     regulatory      1906..2104
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2191..4170
                     /locus_tag="VHV99_18575"
     CDS             2191..4170
                     /locus_tag="VHV99_18575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011355088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX3637450.1"
                     /translation="MSTAVQTGSPQGTLRRHVVGMYSVLAVLSVSAWQVAQAADDTSS
                     PPPAQASAETTLPNILVTGDTQHLPQSFDQRYATTQVLTRADLERLSPANPSITQALA
                     TLPGVTVSQNGGPGSFASVSIRGSSGSQVAVFIDGIRVGSATTGQAAWADLSTSSFDR
                     VEVISGPAAASFGAAAMGGVVQLFTNHASNQPNQTTISVGGGSNKTFDTQVRTSGSIP
                     STGPLSALSGLTYSLGLHDYNTAGIDATQPYAFGHEDGRNPYHAQDVDARLGYARDNW
                     SLSTFALYHKSDLSYDSAGGDDRQLDHQLTTGLAFHLDITPTTQFDQSVGYATDRQFL
                     YANNPTIPTDEIDSTRFSTSTSLTHQEKGLTLFGLPLSGETKLAYDFSREQAFLPVDI
                     PQGVPTRNDSAVSLHQSTTLGALTVFLAGRHEIIQGRSVNTGNVAVSYAITPVYTARL
                     SYGNAFRLPTFNDLYYPGFANPNLLPERSDTIEAALDATTSFGTFTAAVYDTRVHDLI
                     SYNSATFLPVNIGRAHIQGIDLSYKGTLGKSTPVSLAIGLLNPQDVTDQTWLNRRPRQ
                     TVSLSVDHTWDEFHLHALSTGVSLLYGGSTFDDQLNSIYLPSYTTVALRAAYKVNSHL
                     TVSANLSNLFNRQYVTAYGYNTLGRTAFGKLAYTF"
     gene            4356..4802
                     /locus_tag="VHV99_18580"
     CDS             4356..4802
                     /locus_tag="VHV99_18580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX3637451.1"
                     /translation="MTTGTVNLHRVLRATPERIYRAFLEPDAIAKWLPPYGFTCQVHQ
                     LDARVGGTHKMSFRNFTTGNGHSFGGEYVELVPFEKIRYTDRFDDTNLPGEMHVTVSL
                     RQVVCGTEVAITQAGVPEVIPVEMCYLGWQESLAQLAKLVEPEIPD"
     gene            4948..5985
                     /locus_tag="VHV99_18585"
     CDS             4948..5985
                     /locus_tag="VHV99_18585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX3637452.1"
                     /translation="MTIKRRSTISELAELAGVHASTVSRVMNPATRHLIGAEVADRVL
                     KAAQKLNYSPNRIAATLRTSRSGTIGVILPDITNPIFPPICRGIEDELAKRGMLPLIA
                     NAAGTMERKRFVVDQMVGRRVDGLILATAERNDPVLMHCLEEGLPVVVVNRSDDSGRV
                     SCVVGDSHLAMRLAVAHLTQLGHSHIAHVGGPIELSTGFQRKQGFLDALKENNLKLSA
                     CPVVQSDAYSRPAGKAACSALLEKRKKITAIVAGNDLIALGCYDALHAAGLRCPDDVS
                     VIGHNDMPLMDSVAPAMTTIHLPLYEMGARAAALIIDTLQGSGKGNVNVVLRPELMIR
                     ESTAAPSPTRV"
     gene            6455..7360
                     /locus_tag="VHV99_18590"
     CDS             6455..7360
                     /locus_tag="VHV99_18590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010810275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0018576 - catechol 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0009712 - catechol-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HEX3637453.1"
                     /translation="MNEIAPQGIIENADAVTAVVEAAMRNTTNPRLKQILLALVRHSH
                     AFLREVRLTDQEFEQGHAFLKEVGLACNDLHNEVVLLADVLGISTLVTLLNNSNPQER
                     TSGALLGPFFRGGAPRYENGANIVAEGSPGKPLFVRAKVQNARREPIVGAQVDVWQAS
                     PIGLYENQDPEQPEMNLRGQFRTNEHGEFYFRSVRPSGYHVPTHGPVGPLLAAQQRHP
                     YRPAHIHFVVIADGYDTLVTQVFADDSDHLNSDVAFGVTRDLIGRYELRETGAPEPVD
                     GSFYTLDYDFVLAEGTPTYPVPPIK"
     gene            7384..8130
                     /locus_tag="VHV99_18595"
     CDS             7384..8130
                     /locus_tag="VHV99_18595"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011301959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3637454.1"
                     /translation="MSDSLEGKSVVVIGGTGGIGAATSRRFAAAGARVVVAGQSVERA
                     QELVAQLPGTGHFAAKVFIEKSDTLKQLAEDVAGRYGHVDVLVNAAGFTRPVPHADLD
                     ALTDELIDDIMRVNLRAQFAAVRVFRALLERSGAGLIVNVSSISGSNGIGSNIAYCAA
                     KAGLDVMTMSLARVLAPRVRILNVSPGVVDTGFVPGRSADFNDKVAGTTPLRRIGSAD
                     DIAQAIEACATQLTFSTGTTIVVDGGRRLG"
     gene            8221..9099
                     /locus_tag="VHV99_18600"
     CDS             8221..9099
                     /locus_tag="VHV99_18600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011301958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEX3637455.1"
                     /translation="MHSRKTIITCAITGNLTKPEQHPGLPITPAQIAQSALEAAAAGA
                     SVAHIHVRDPATGQPSMELDLYREVIQLIRAADQQLIINLTTGPGGRFVPGEDDPRIA
                     GAGTTLMRPEKRVEHIAALKPDICSLDLNTMNSGAQVVINTPRNVTRMAHIIREAGVK
                     PELEVFDTGDIHLGLDLIKDGVLDGPGLWTLVTGVKYGFSATPETLFYARNLLPAGAV
                     WSAFGIGRYEFPIVGQSWLAGGHVRVGLEDNIYFSRGVLAETNAQLVARARDIVLSMG
                     GEIASAREARDLLELS"
     gene            9096..9647
                     /locus_tag="VHV99_18605"
     CDS             9096..9647
                     /locus_tag="VHV99_18605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006156211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEX3637456.1"
                     /translation="MNVLESFAHLRVRPCVHSSAFLAAGAVVIGDVEVGAHSSIWFNC
                     VLRGDVQRISVGAGTNIQDGSIVHCSTNGRPTIIGDAVSVGHGAILHSCTIEDTGFVG
                     FGARVLDGAVIRRGGMLAAGAVLTPGKVVGTGELWAGNPAKLLRVMDSNEVSALGAIA
                     ERYTALALAYQNGRTENIIYREK"
     gene            9649..10626
                     /locus_tag="VHV99_18610"
     CDS             9649..10626
                     /locus_tag="VHV99_18610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008651611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEX3637457.1"
                     /translation="MPQAITVKAKRANIDALELNFVEQSRPAINAGECLVEVGSSGVN
                     PSDVKAVLGFMPHAVWPRTPGRDYAGVVVDGPAALLGKTVWGSGGELGIRNNGAHASY
                     VVVPVASVREKPAALSMDEAGAIGVPFVTAAEGLRRAGGAAPGKVVLVLGANGKVGQA
                     AIQQATLAGAQVFGVERNAQPYVGHASGNVTMIESVENIDTVVRDLTGGHGADIVFNT
                     VGSPYFDKANRAMAMRGTQIFISTIDRAVPFDILEFYRGQRTYVGVDTLALDSVEGAA
                     ILDSLAGSFESGALKPFPIVDDHVFSLDKAADAYRIVFNGAQHRVLLKP"
     gene            10623..10958
                     /locus_tag="VHV99_18615"
     CDS             10623..10958
                     /locus_tag="VHV99_18615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008651613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX3637458.1"
                     /translation="MNVTRFAKAPPYFPDNHFGMQCVRLQGHEAGPSQSLWLGVSTIE
                     PGGHTTLSGSRVEKHYVVLEGTVNVQTDTGEVALERHDSCRLEPNERRALHNRTQQPA
                     LILLAMPYA"
     gene            11165..12376
                     /locus_tag="VHV99_18620"
     CDS             11165..12376
                     /locus_tag="VHV99_18620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008168171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3637459.1"
                     /translation="MSDAGNKTLTGGWVLFALALGAFAIGTTEFATMSLLPMFARDLG
                     IDAPTAGHVISAYALGVVVGAPVIAVVAARIARRTLLTILMGMFALANGMSGLAPTYH
                     WMLVFRFLSGLPHGAYFGAAALMAISVVPENRRTAAVGRVFLGLTSATILGVPLANWL
                     GQAVGWRWSFGLVALLGVLTMALVRLLAPAQPADAKSSQLREIRALGRVQVWLTLGIG
                     AIGFGGMFAVYTYLANVLGAVTGVSAQSAPVVLSVLGLGFTIGNIVVPMLADRALMRT
                     TGAVLVWSAASLALFPLMAAHVWTITLDVFLIGIGGSLGALLQTRLMDVAGDAQGLAA
                     ALNHSAFNTANALGPFLGGLAISAGFGWTAPGWVGSLLAIGGLVLWLVSAAVERSRNR
                     EVATIRENIPG"
     gene            complement(13338..13721)
                     /locus_tag="VHV99_18625"
     CDS             complement(13338..13721)
                     /locus_tag="VHV99_18625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX3637460.1"
                     /translation="MREEQIREALTAHWRASAAGDLDAEHDIYHDDVICDYPQSGERI
                     AGRTNLQALRGHHPDKPSGFDVRRIQGEGNFWVTEYSIAYTGRAAYTVSIMEFRNGKV
                     VHETQYFSDPFEAPAWRSQWVQPIA"
     gene            13892..14197
                     /locus_tag="VHV99_18630"
     CDS             13892..14197
                     /locus_tag="VHV99_18630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011003256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="panthothenate synthetase"
                     /protein_id="HEX3637461.1"
                     /translation="MRMLLNIRIPHEPFNAFVRDGSIGELIERILADAKPEAAYFTEQ
                     NGTRGAILIVNIEEPSQVPRFAEPWFLTFNAACEFRVVMVADDIKKAGLEALGSKWK"
     gene            complement(14349..15065)
                     /locus_tag="VHV99_18635"
     CDS             complement(14349..15065)
                     /locus_tag="VHV99_18635"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3637462.1"
                     /translation="MNDSIQADLDRPIWAALTTKQAHLGQGDALARRYHPDVAPFAAL
                     ASETPAAYRTLRQLLRPHEQVVLQSAEPLGPVDGLQATYLGVIHQMIAPHREAGGTDD
                     PGVIRLGNAEANEMLDLVQKTKPGPFGKRTCEMGNYIGIRDGGRLIAMAGERMRVDGY
                     VEISAVCVDEDYRGKGLAGRLVKALRREIEQRGETPFLHVFSDNISAIRLYERLGFRL
                     RYTFHLNRIGHADPGSAAVL"
     gene            15178..16140
                     /locus_tag="VHV99_18640"
     CDS             15178..16140
                     /locus_tag="VHV99_18640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011549100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3637463.1"
                     /translation="MRLVIFALDGLFDTGLTVLLDTFATANELAAAQGFAAPPFDVRL
                     VGVRRRIRTAHGLSTPVEAASTVPRPDWVVVPAPNVRQPERLIEALARPDVRDAMTYL
                     RTWHADGVGVAAACIGTFLLAEAGLLDNREATTTWSLAPFFRQRYPAVNLDDSRMVVA
                     SHRVVTAGSAMGHLDLALWLLRQASPELATLVARFMLIDKRSSQAEYIIPDHLAHADP
                     LVARFERWARDNLSSGFSLQAAAAALSVGPRTLQRRTEAVLGKSPLAFFQDLRIERAQ
                     NLVSIGYDLETIASEVGYADSATLRSLLRRRLGRGVRELRAEMR"
BASE COUNT         3161 a         5096 c         4803 g         3331 t
ORIGIN      
        1 tgcgcgtcag tttgaggtgg cggtcaactc gacgcgtccg tgatctgaaa cagcgattcg
       61 agccgctgtc gtcgctacgg gtctgataac tagcggggtc tgctcatcag cttgaggtgg
      121 gtaaccaacc agacaactag acgcgtacga tcttggaggg gcggttcgag ccgacgtcgt
      181 tgctagcagg tatctaatag ttggtggcac ttgcgcctca gcttaagttt ggccattgtg
      241 tcgcccgcgc ccacttagaa gctgttgcga aattagttaa acgtaggctt gagtcgcgtc
      301 cagcaaatca aggcgcaagc cagggacaga aaggcttcat ggacatgggc ataacgttcg
      361 taacgaatct tcaggcgtag acacgagtga agccatgcaa tcgagcgctc gactggccaa
      421 cgcgttttgc ccagaccact gccatggggc gagccaatct ttgcgagcat cggcgtgatg
      481 ccgcgctttc tcaaacgctg tcgatgtgct tccgagctgt agccccggtc gccctgaacg
      541 atttgcggtt tatgcagtgg gcgtccccgc ttgcctcgaa tgtgtggaat ggcatcgacg
      601 agtgcatcaa gctgggtgac gtcgtggcgg ttcgccccgc tcaggatgac cgcaagcggg
      661 atgcctttcg cgtcgacgat gaggtggtgt ttgctgccga gcttgcgccg gtccgtggga
      721 ttcgggccgg tttttttccc gccagcatcg cgcgaatcga cgaactgtcc acaagcgctc
      781 gtgacaggtc gatttcgcca cgccgacgca gttcggccag caacgtttcg tggatgcgtt
      841 gccagactcc ggcttcttgc catgccacga gccgtcgcca gcaggcggtg cccgaaccgc
      901 atcccatctc ttgtggcagc agattccacg caatgcccga gcgcagcaca aacacgatgc
      961 cggttagaac ggctcggtcg ggtgtcggct ttcgacctgc gtattggcga ttgcgcggtg
     1021 ctcgtgcagg caacaacggt tcgatgagtg accacaattc gttgtctatc agttctttag
     1081 gcatcgcttc ctgtcaggca aaacaagaca gaaagttaac accaagtcgg aaaagttaac
     1141 agacccaaaa cttaatttcg caacagcttc ttaggtgctt tgccagccga atttaattta
     1201 tttggtaatc gaagggcggt gtgcggccct tcgtcacgtg tcatcgcccg attataaatt
     1261 cgaggtgttc ggcccggagc ggtttctgcg tcgttggcgc tcagcttcct gtgcgagcca
     1321 tacgccgcgc agttcgtcat tctcggcctt gcgctcaatg gtccgttgtg cgcgctcacg
     1381 cgccttggcg gcctcatcgt attcaggggt caccatgagg ctttgccggc tgtagtcaac
     1441 cgagacatag acgcgctggc cggccctgaa gaacttctcc gcgcctagcc accggtcgat
     1501 gtgttcccag ggcatgaagg gctgctgcgg ttgcgtgact gttgcagtcg atggcgcgtc
     1561 gggcgcatcg cgtagcgtcg tgcttatgca taaacggcgc tgcactacga ccggcatcgg
     1621 tactatgatc cgacgacgga gcggttcatc agcaaggact cgatagggct cgcgggcggc
     1681 atcaagctct atcaatatgc aaacaacccc gcactgcggc cctctcgttt ccacccggca
     1741 atgcacagag tccatgccac gaaaacgccg catcaaaacc tcacccaaaa aaccattcgc
     1801 caacctatat caacgcatcg cctgatccga acaagaaaag caaaatcaca gtgacgaaca
     1861 gcgtaccgca aacgccaaaa taattgacgt caccaccacg catcgctacc cttccgcctt
     1921 gatggttccc gtcaaccggg atcaaaaggg aacgcagtac gagcagcgat gctccgagtc
     1981 tgcggctgtc cccgcaactg taagcggcga gtccgtgcca ttcatgacca ctggagcacc
     2041 gggaaggtcg gcacgggacg ttgacccgtg agccaggaga cctgccatca aaccgaggtc
     2101 tgcgcagccg catcgggcgg ggtgtcccga tgcgcaagca ccgcctatgc ggcgcccgtt
     2161 gcaacgacag cccaagcctc aggatcagtc atgtccaccg ccgttcaaac cggttccccg
     2221 cagggaacct tgcgccgaca cgtcgtcggc atgtatagcg ttctcgccgt gttgtcagtc
     2281 tccgcatggc aggttgcaca ggccgcggac gacacatcgt cgccaccgcc cgcgcaggcg
     2341 tcggccgaaa ccacgctgcc gaatatcctt gtcaccggcg acacccaaca cctgccgcaa
     2401 tcgttcgacc aacgctacgc cacgacccag gtcctcacgc gcgccgatct cgagcgtctg
     2461 tccccggcca acccgagtat cacgcaagcg ctcgccacgc tgcccggcgt caccgtttcg
     2521 caaaacggcg gccccggctc cttcgcttca gtcagcatcc ggggttcatc cggcagccag
     2581 gtcgcggtgt ttatcgacgg catccgggta ggctccgcaa ccaccggcca ggccgcgtgg
     2641 gcggatctct caacctcctc gttcgaccgt gtcgaagtca tctcagggcc ggcggccgcg
     2701 tcgttcggtg cggccgcgat gggcggcgtc gtgcagctgt tcaccaatca cgcatccaat
     2761 cagccgaacc agaccacgat ctccgtaggc ggcggttcga acaaaacctt cgacacgcaa
     2821 gtccggacct cgggcagcat cccctccact ggcccgctct cggcactgtc cggactgacc
     2881 tactcgctcg gtctgcacga ctacaacacc gcagggatcg atgcgaccca gccgtatgca
     2941 ttcggtcacg aggatggccg aaacccgtac catgcccagg acgtggatgc gcgcctcggc
     3001 tatgcgcgcg acaactggtc gctctccacg ttcgcccttt accacaagtc cgatctctcg
     3061 tatgacagtg ccggtggcga cgatcgccag ctcgatcatc agttgacgac gggcctcgcg
     3121 tttcacctgg acattacgcc gaccacccag ttcgaccagt cggtgggcta cgccacggac
     3181 cgccagttcc tctacgcgaa caatcccacc attccgaccg atgagatcga ctcgacgcgt
     3241 ttcagcacgt caacctcgtt gacccatcag gaaaaggggc tgacgctgtt cggcctgccg
     3301 ctgtccggag agacgaagct cgcctacgac ttcagccgcg agcaagcttt cctgccggtc
     3361 gatatccctc agggcgtgcc gacgcgcaac gattcagcgg tctcactcca tcagtcaacc
     3421 acgctcggtg ccctgacggt gttccttgcg ggccgtcacg agattattca gggccgctcg
     3481 gtcaataccg gcaatgtcgc cgtgtcctat gcgatcacgc cggtttatac ggcgcggctt
     3541 tcgtatggca atgcattccg cctgccgacc ttcaacgacc tgtactaccc aggctttgcg
     3601 aacccgaacc tgttgcccga gcgcagcgac acgatcgagg cggcgctgga cgcaaccacc
     3661 tcgttcggca ccttcacggc agccgtttat gacacccgcg tccatgacct catctcgtac
     3721 aactccgcga cgtttctgcc cgtgaatatc ggacgggcgc acatccaggg catcgatctg
     3781 tcgtacaagg gcacgctcgg caaatcgacg cctgtcagtc tcgcgatcgg cctgctgaat
     3841 ccgcaggacg tgaccgatca gacgtggctc aaccgccgcc cgcgccagac ggtcagtctg
     3901 agcgtcgatc acacgtggga cgagttccat cttcacgcgc tcagcaccgg cgtctcgctg
     3961 ctttacgggg gatcgacctt cgacgaccag ctcaactcga tctatctgcc gtcgtacaca
     4021 acggtcgctt tgcgtgcagc ctataaggtc aattcgcatc tcaccgtgtc ggccaatctg
     4081 tcgaatctgt ttaaccgaca gtacgtcact gcatacgggt acaacacgtt gggacgtacg
     4141 gcattcggca agctggctta tacgttctaa gcggcaaatg gccctcccgg tggcatgaac
     4201 ggctgggagg gcgccagttc cggtcttcag gctgaacaga tcgatggacc attgcttgcc
     4261 cagggttttt cctggtctcg acctatgtcg atatccggat catcgaatcg tcgtaggcgt
     4321 atgaccaact tctccaagtg aaaggcgaac acaccatgac tactggaacc gtcaatctcc
     4381 atcgcgtctt gcgcgctacg cccgaacgca tctatcgggc ctttctcgaa cccgacgcga
     4441 tagcgaagtg gctgccgccc tatggcttca cctgccaggt ccatcaactg gatgctcgcg
     4501 tgggtggcac tcacaagatg tccttccgca atttcaccac cggcaacggc cactcgttcg
     4561 gcggcgagta tgtcgaactg gtgccgttcg agaaaatccg ctacacggac cgattcgacg
     4621 atacgaacct gcctggcgag atgcacgtca ccgtttcatt acggcaggtg gtctgcggga
     4681 ccgaggtcgc catcacgcag gcgggcgtgc ccgaggtcat tcctgtggag atgtgctacc
     4741 tcggctggca ggagtcgctt gctcagctgg ctaaattggt tgagcctgag attccggatt
     4801 gactgacggc gcgggcatgg ggatgcggtc tgttgttcgg gtagcgcccg cttgttgatt
     4861 tagcccacgc gccgcaacat tcgatcgccg gcgaattgca acgggctaaa atagggcgtt
     4921 gcccttttcg cacagacgtg cccgtccatg acgatcaaga gacgctcgac aataagcgag
     4981 cttgcggagc tagccggtgt tcatgcatcc accgtgtccc gcgtgatgaa cccggccacg
     5041 cgccacctga tcggcgccga agtcgcagac cgtgtgctca aggccgccca gaagctcaac
     5101 tactcgccta accggattgc ggccaccttg cgaaccagcc gctcgggcac catcggggtc
     5161 atcctgccgg acatcaccaa cccgatcttt ccgcccatct gccgcggtat tgaggacgag
     5221 ctcgccaagc gaggcatgct gccgttgatc gcgaatgcgg cgggcacgat ggagcgcaag
     5281 cgcttcgtcg tcgatcagat ggtgggccgg cgcgtcgacg gactgattct cgccaccgcc
     5341 gagcgaaacg acccagtgct catgcattgc ctggaggaag gacttccggt cgtcgtcgtc
     5401 aaccgcagcg acgatagcgg ccgcgtgtcc tgcgtggtcg gcgacagcca cctggcaatg
     5461 cggctggccg tcgctcatct gacccagctt gggcacagcc acatcgccca cgttggcggc
     5521 cccatcgagc tgtctaccgg tttccagcgc aagcaggggt ttctcgacgc gctcaaggaa
     5581 aacaacctga aactgagtgc gtgccctgtc gtgcaaagcg acgcttattc acgtccggca
     5641 ggcaaggccg cttgcagcgc gctgctggaa aagcgcaaga aaatcacggc gatcgtcgcg
     5701 ggcaacgatc tgatcgcgct cggctgctac gacgcgctgc atgcggcagg gttgcgctgt
     5761 ccggacgatg tttccgtgat tggtcacaac gacatgccct tgatggattc cgtcgcgccc
     5821 gccatgacca ccattcatct gcccttgtac gagatgggcg caagggctgc cgcgttgatc
     5881 atcgacacct tgcagggaag cggcaagggg aacgtcaacg tcgttttgcg gcccgaactg
     5941 atgatccgcg agtcgactgc ggcgccctcg ccgacgcgcg tgtaaagcgc gccgttcgcg
     6001 cggtctggca caaaggtgtg cccttgatca ggatctcgcc gcgaacgtca ccccgcgtct
     6061 cgtccatggt cacgacaagt gcgttcgtga tcagtgtgta agtcatttgt actctcttcc
     6121 cgcctgccga taacggtacc gcttcaacga acgaccggcg cgcggcacca tgaattgacc
     6181 ccttcaaacg ctaatcattt acgcaatcga ttgcgctaat gtacgcttgc cgccaaatcc
     6241 caaaaccaag tgtttgccca tatccgcatt cttgtcgtaa agcgcaagac acttttttgc
     6301 ctggcaggtt tgcgggaaat ttctgaccaa aaaccatcgt cggagtccac ctgccttgag
     6361 aactcgttgg caccgagggc gcgggtcgac gcttgacagt cgcaaatatg cgtcatagca
     6421 tgcaatcgat tgcacgaact gacgagaagt gaacatgaac gaaatagctc cacagggcat
     6481 catcgaaaac gccgatgccg taaccgcagt cgtggaagct gcgatgcgca acacgaccaa
     6541 tccgcgcctg aaacagatcc tgcttgcgtt ggtcaggcac agtcacgcgt ttctccgcga
     6601 ggttcgtctt accgaccagg agttcgaaca aggacacgcg ttcctgaaag aagtcggcct
     6661 ggcatgcaac gacttgcata acgaggtggt tctgctcgcc gacgtcctcg gaatctcgac
     6721 gctggtcacc ctgctgaata attccaaccc gcaagagcgt acctccggag cactgcttgg
     6781 accgtttttc cggggtggcg cgccacgcta tgaaaacggc gcgaacatcg tcgccgaagg
     6841 ctcgccgggc aagccgcttt tcgtcagagc gaaagtgcag aacgcacgcc gcgaaccgat
     6901 tgtgggtgca caagtggacg tgtggcaggc ttcgccgatc ggcctttacg aaaaccagga
     6961 tccggagcag ccggaaatga atctgcgtgg ccagtttcgt acgaacgagc acggcgaatt
     7021 ctatttccgc agcgtccgtc cgtccggtta tcacgtgccg acgcacggac cggtggggcc
     7081 cctcctcgcc gcccagcaac ggcatcctta ccgcccggca cacattcact tcgtcgtgat
     7141 tgcagatggc tacgacacgc tcgtgacgca ggtgttcgcc gacgattccg accatctgaa
     7201 ttccgacgtc gccttcggtg tgacgcgcga cctgatcggc cgctatgagc tgcgcgaaac
     7261 gggggcaccg gagcccgtcg acgggtcctt ctacacgctt gattatgact tcgtgctggc
     7321 ggaaggcacg ccgacctacc ccgttccccc gatcaaataa acaccgctat aaagaggctc
     7381 aagatgtccg actcacttga aggaaaaagc gtagttgtta tcggcggtac cggtggcatc
     7441 ggtgctgcaa cgagccggcg ctttgccgcc gcgggcgcac gcgtcgtcgt cgcgggccag
     7501 tccgtcgagc gcgcgcagga gctcgtcgcg caattgcccg gcaccggtca cttcgcagcc
     7561 aaagtgttta tcgaaaaaag cgacacgctg aagcagctcg ctgaagacgt ggccggccgt
     7621 tatggccatg tcgacgtgct cgtcaatgca gcgggattca ccaggccggt cccgcacgct
     7681 gacctcgacg cactgaccga tgaactcatc gacgacatca tgcgcgtcaa cctgcgtgcc
     7741 cagtttgccg ccgtgcgcgt ctttcgtgcg ttgctggaaa ggtccggcgc aggcctgatc
     7801 gtgaatgtgt cgtcgatctc cggatcgaat ggcatcggca gcaacatcgc ctattgcgcg
     7861 gcgaaggcgg gtctcgacgt gatgaccatg tcgctcgccc gcgtgctcgc gccgcgggtg
     7921 cgcatcctga acgtgtcgcc gggcgtggtg gacacgggat tcgtaccggg ccgcagcgcc
     7981 gacttcaacg acaaggtcgc cggcacgacg ccgttacgcc gcatcggcag cgctgacgac
     8041 atcgcacagg ccatcgaggc gtgcgcgaca cagctgacgt tcagcacggg caccaccatt
     8101 gtcgtcgacg gcggacggcg cctcggctag tcaggcaaac cggtccgcgt cacgagggcc
     8161 ggcatcggct ttgtcccggc gcgtctgcgt gtcacaaccc gctacaagag agtcttactg
     8221 atgcattccc gcaaaaccat cattacctgc gccatcacgg gcaacctgac aaagccggag
     8281 caacatcccg gacttccgat aacgcctgcg caaattgcac aatccgcact tgaagccgcc
     8341 gctgccggtg cttcagtcgc ccatattcac gtgcgggatc ccgccacggg ccagccctcg
     8401 atggaactgg atctgtaccg cgaagtcatt cagctgattc gcgccgctga ccagcaactc
     8461 atcatcaacc tgaccacagg tcccggtggc cgcttcgtac cgggcgagga cgatccgagg
     8521 attgccggcg ccggtacgac gctcatgcgt ccggaaaagc gcgtcgaaca tattgcagcg
     8581 ttaaagcccg atatctgcag ccttgacctg aatacgatga actccggcgc acaggtggtg
     8641 atcaatacgc cacgcaatgt cacgcgcatg gctcacatca tccgtgaggc aggcgtcaaa
     8701 ccggaactcg aagtgttcga caccggcgac attcatctgg ggctggatct catcaaggac
     8761 ggcgtgctcg acggccccgg cctgtggacg ctcgtcaccg gcgtgaaata cggcttttcg
     8821 gcaaccccgg agacgttgtt ctatgcgcgc aacctgttgc cggccggcgc cgtgtggtcg
     8881 gcgttcggca tcggccgcta cgagtttccc atcgtgggtc aatcgtggct ggcaggcggc
     8941 cacgtgcgcg tcggtctcga agacaacatt tacttctccc gcggcgtgct tgccgagacc
     9001 aacgcgcagc tagtggcccg ggcacgggac atcgtgctgt cgatgggcgg agaaatcgct
     9061 tcggcgcgcg aagcgcgtga tctgctggaa ttgtcatgaa cgtgctcgag tccttcgccc
     9121 atctgcgcgt gcggccgtgc gtgcattcga gcgcgttcct cgcagcgggc gccgtcgtca
     9181 ttggcgatgt cgaggtcggc gcacactcct cgatctggtt caactgcgtg ctgcgcggcg
     9241 acgtccagcg tatttcagtg ggcgccggca ccaacatcca ggatggatcg atcgtgcact
     9301 gctcgaccaa cggccgcccg accatcatcg gcgatgccgt gtccgtggga cacggcgcca
     9361 ttctgcattc ctgcacgatt gaagacaccg gctttgtcgg cttcggcgcg cgcgtgctcg
     9421 atggcgcggt gatccgccgc ggcggcatgc tggccgccgg cgcggttttg acaccaggca
     9481 aggtggtcgg cacgggcgaa ctgtgggcgg ggaaccccgc caagctgctt cgcgtcatgg
     9541 acagcaacga ggtgagcgcg cttggcgcga tcgccgagcg ctatacggca cttgctctcg
     9601 cctaccagaa cggccgaacc gaaaacatca tctacaggga aaagtaacat gccacaagca
     9661 ataaccgtta aagcaaagcg cgcgaacatc gatgctctcg agttgaattt cgtagaacaa
     9721 tcccggccgg ccatcaatgc cggcgagtgc ctcgtcgagg tcggcagctc cggcgtcaat
     9781 ccgagcgatg tcaaagcggt gctgggcttc atgcctcacg ccgtctggcc ccgcacaccg
     9841 ggccgcgact atgccggcgt cgtggtggac ggtcctgcag cgctgcttgg caagacagtc
     9901 tggggcagtg gcggcgaact gggcattcgc aacaatggcg ctcacgcaag ctatgtggtc
     9961 gtaccggttg cgagcgtgcg tgaaaagcct gccgccctgt cgatggacga agccggcgcg
    10021 atcggcgtgc ccttcgtgac cgcagccgaa ggcctgcgcc gcgcgggcgg tgccgctccc
    10081 gggaaagtgg tgctcgtgct cggcgccaac ggcaaggtcg gccaggccgc gatccagcag
    10141 gccacgctgg caggcgcgca ggtgttcggg gtcgaacgca atgcccaacc gtatgtcggt
    10201 cacgcgagcg gcaacgtcac gatgatcgaa tctgtcgaga atatcgacac cgtcgtgcgc
    10261 gatcttaccg gcggtcatgg cgccgacatc gtcttcaaca cggtgggcag cccttacttc
    10321 gataaggcaa accgggcgat ggcgatgcgc ggaacacaga tcttcatttc aacgatcgat
    10381 cgcgcggtgc cgttcgacat tctcgagttc tatcgcggcc agcgcaccta cgtcggcgtc
    10441 gacacgctcg ccctcgacag cgttgaaggc gcagcgattc tcgacagtct ggccggttcg
    10501 ttcgaaagcg gcgcgctcaa gccgttcccg atcgtcgacg accacgtttt ctcgctcgac
    10561 aaggcagcag acgcctatcg gatcgtgttc aacggcgcgc agcatcgcgt gctgctcaag
    10621 ccatgaacgt cacgcgcttc gccaaagcac cgccctactt tccggacaac cacttcggta
    10681 tgcagtgcgt gcgtctgcaa ggacatgagg cggggccgtc gcagtcgctc tggctcggcg
    10741 tgtcgacgat cgagccaggc ggccacacga cgttgtcggg ctccagggtc gagaagcact
    10801 acgtcgtcct cgaaggcacc gtcaacgttc agaccgacac cggggaagtc gcactggagc
    10861 ggcatgactc gtgccgcctc gaaccgaacg agcggcgcgc gctgcacaac cgcacgcagc
    10921 aaccggccct tattctgctg gcgatgccct acgcttaagc gctgatacga tccacgcgcg
    10981 acaccgttcg tgtggctatt tcatggccgc atgaacggcg cgcgcgagcg cttcaggttt
    11041 cggttgagta tgtttgcgca ggcttaaagc cgacagcaaa cagcggcgcg cggttaggtc
    11101 cgcatgagcc cgcgtcgtat ctggaatgat tgatgaggtt gtttaggcaa tctgaggaca
    11161 aacaatgagt gacgctggta acaaaacgct aacgggcggt tgggtcctgt tcgctctcgc
    11221 ccttggcgcc tttgccatcg gaaccaccga gttcgcgacg atgagcctgt tgccgatgtt
    11281 cgcgcgagac cttggaatcg acgcgccgac tgcgggtcat gtgatcagcg catacgcgct
    11341 gggcgttgtc gtgggcgctc cggtcattgc ggtcgtggcg gcgcggatcg cccggcgcac
    11401 gttgttgacc atcctgatgg gcatgttcgc gcttgcaaat gggatgagcg gcctggcgcc
    11461 cacttaccat tggatgctgg tcttccggtt tctcagcggg cttcctcacg gcgcatattt
    11521 cggcgccgcg gcgctgatgg caatctccgt cgttcctgag aatcgacgaa cggcagcggt
    11581 cggacgcgtc tttcttggct tgacgtccgc gaccatcctg ggggtcccgc ttgccaactg
    11641 gttagggcag gctgttggat ggcgctggag ttttggcctc gttgcgttgc tcggcgtgct
    11701 cacgatggca ctggtacggc tattggcacc ggcgcagccg gccgatgcga aatccagcca
    11761 gctgcgcgag atccgggcac ttgggcgcgt acaggtgtgg ctgacgctcg gcatcggtgc
    11821 gatcggcttc ggtggcatgt ttgccgtcta tacgtatctg gccaatgttc tcggcgcggt
    11881 gaccggcgtt tccgcgcaat cggctccggt cgttctaagc gtccttggct tgggctttac
    11941 gatcggcaac attgtcgtac ccatgctcgc cgatcgggct ctgatgcgca ctaccggcgc
    12001 ggtgctggtt tggagcgccg cttcgctggc actattccca ttgatggccg cccatgtctg
    12061 gaccatcacc ctcgatgttt tcctgatcgg tatcggcggc tcactgggcg cgcttctcca
    12121 gacacggttg atggacgtgg ctggagatgc ccagggcctg gcagcagcgc tcaatcactc
    12181 ggcgttcaac acggccaacg cactcggacc gtttctcggc ggcctggcca tctcagcggg
    12241 tttcggatgg acggcgccag gatgggtggg gagtcttctc gccatcggcg gcctggtgct
    12301 ctggctcgtt tccgcggcgg tcgaacgtag ccggaatcgc gaagttgcca cgatccgcga
    12361 aaatattccg ggttgaatgg cccggtaatc gggtttccga ttgacatagc cggtcaggcg
    12421 ccaaccagga ctattggccg gtgacttatg tctgtattac agctgcgatg aggacggaga
    12481 tggcgaggac ccacaactcg acggcggtga agcgggggta tctaatgttc atcatcatcc
    12541 tcgcgcgccg cagcaggaac ttcatcgggt acttgtcctc tatcgttacc gtttggtctg
    12601 tcgcattgat atactcccct attgcagggc ctccatgcgc aactcaatct tcctgatcag
    12661 gagatgaaca gttcgatccc atcgcagtca tcgctggccg ctgggagact gcaaatccgt
    12721 gccagtccgc ggtcatttcg gcgaagcaat ccgcacagca gacagcggta atattcggcc
    12781 aaaggctcac gtgcttccga acgtcctcct tcaactcgtc gcattcatcg cagcgatcca
    12841 gcacacggac ctgcagcgtt cggttgtgcc acttttcgtg cgcgttcata tcagcagccc
    12901 tcgtgttgat cggcgatgtc gacggcgatg atgttcgcgc ccatcctccg cagttcggcg
    12961 ggcgccgtat cgcctcggcg cggctcgagc aaatctgttg cgttcgtcct catcatggtc
    13021 ctcaataaat acagatgctg ccaggttcag cagtccggcg atatacgttc ttattcaacc
    13081 agaggacgaa cgtgtattcg tcgtacgctt tgcgactgat ataaacccag tatgcagagc
    13141 gtttgacatg gctgacattc aaatgaaatc gctaagtcca tggaatcaag atagacgcac
    13201 gactcccaga aaaccattct acgaacggtt gctgcggttc tacatggaca tagttcgctt
    13261 atacgaatgg ataactcgag ggagccagga gcagcggctt ttgttgtgct cgtgacggct
    13321 ctcgccgcaa cgcgatgtca cgcgatcggc tgaacccatt ggctgcgcca ggccggtgct
    13381 tcaaagggat ccgaaaaata ctgggtctcg tgaacgacct tgccgttgcg gaactccata
    13441 atgcttaccg tgtaagccgc tcgccccgta taggcgatgc tgtattccgt gacccagaaa
    13501 ttaccttctc cttgaattcg cctgacatcg aaacctgatg gcttatctgg atgatggcct
    13561 cgtagtgcct gcaaattagt tcgcccggcg attcgttcac ctgattgcgg ataatcacag
    13621 atgacatcgt catggtaaat atcgtgttcc gcgtcgagat cgccggctgc cgacgcgcgc
    13681 cagtgcgcag tcagggcttc acgtatttgt tcttcccgca tggcgtgcct ccggataccg
    13741 ttcacgtcta tctatcgcca aacaaggtct gcttctcgat caagtgtagc aaatggatga
    13801 cggggcgggg tttgttgaat atccacgggc aatgacgcga caaactccta ttcttgaaga
    13861 gtccatccgg acacaatcaa ggaggaaggc tatgcgcatg cttctcaaca tacgaatacc
    13921 tcacgagccg ttcaacgctt ttgtgcgcga tggctccatc ggagagttga tcgaaaggat
    13981 tctcgcagac gcgaaaccgg aggccgccta ctttactgag cagaacggaa cacgcggagc
    14041 aatcctcatc gtgaacatcg aggagccgtc gcaggttcca aggttcgcgg aaccatggtt
    14101 tcttacgttc aatgccgcct gcgagtttcg cgtggtgatg gtggcagatg acatcaagaa
    14161 agctggactc gaagcgcttg gctcgaagtg gaaataggga gcgcggcacc ttcggctacg
    14221 tcgtgcgggt tcggagcgtt tgggcaagct gtgcaaaccg tcccgtccgc cgacgattcg
    14281 ttgaactcaa ccactatggc cgcccggatg accgatggtc ggcttgcatt ccacctttcg
    14341 ctcatgggtt aaagaaccgc agccgaaccc ggatcagcat gtccaatgcg gttgaggtga
    14401 aacgtgtaac gcaaacggaa accaaggcgt tcatacaacc ggatcgcgga aatattgtca
    14461 ctgaacacgt gcagaaacgg cgtttctccc cgttgctcaa tttctctacg caaggctttc
    14521 acgagccgac cagcaagacc ctttccgcga tagtcttcat ccacgcaaac ggcgctaatc
    14581 tcaacgtatc cgtcgacgcg catccgctcg ccggccatcg caatgagacg ccccccgtcg
    14641 cgaatcccaa tgtaattgcc catctcgcaa gtccgtttgc cgaaaggtcc aggcttggtc
    14701 ttctggacca gatcgagcat ctcgttcgcc tccgcgttac cgagccggat gacccccggg
    14761 tcgtccgtac ctcctgcttc gcggtgcggg gcgatcattt gatgaatcac gcccaggtac
    14821 gtcgcttgta gcccgtcgac cggaccaagc ggttccgcgg attgcaagac gacctgctcg
    14881 tgaggccgca aaagctgacg gagggtccgg tatgcagcgg gcgtttcgga ggctagagca
    14941 gcgaaaggcg ctacatcggg atggtatcga cgggccagcg cgtccccttg ccccaggtgc
    15001 gcctgcttcg ttgtgagggc cgcccagatc ggacgatcga ggtcagcttg tatcgaatca
    15061 ttcatgctct ctccatgtgt cagtccgcgc atagcttcgc tgcattgctg tgcagtctag
    15121 acctccaacc agcagcgggc tatagtcgta aatgacatta atatggtcat ttacgacatg
    15181 agactggtca tcttcgcgct tgacggcctg ttcgacacgg gcctcacggt cctcctggat
    15241 acttttgcga cggccaacga gctggccgct gcccaagggt tcgccgcacc gcccttcgat
    15301 gtaaggctcg ttggcgtacg cagacgcatt cgtaccgcgc atggattgtc gacgccagtc
    15361 gaagcggcaa gcaccgttcc ccgcccagac tgggtggtgg tgcctgcgcc gaatgtgcgg
    15421 caaccggagc gcctgatcga ggcgctggcg cgtccagacg tgcgcgacgc gatgacgtac
    15481 ctgcgtacct ggcatgccga cggcgtcggc gtcgcggccg cctgtatcgg cacgttcctg
    15541 ctggcggaag ccgggcttct ggataatcgc gaggcgacga ccacctggtc actggcgcct
    15601 ttcttccgtc aacgctaccc ggctgtcaac ctggatgatt cacgcatggt cgtcgcgtcg
    15661 catcgcgtgg tcacagcggg ctccgcgatg ggccatctcg accttgcgct atggttgttg
    15721 cgtcaggcaa gtcccgaact tgcgacgctc gtcgcccgct tcatgctcat cgacaagcgt
    15781 tcatcccaag ccgagtacat cattcccgat catctcgccc acgccgatcc attggtcgcg
    15841 cgattcgagc gatgggctcg cgacaatctt tcttcgggtt tttcgcttca ggcagcggcg
    15901 gctgcgttgt ccgttggacc acgcaccttg cagcgccgta ccgaggcagt gcttggcaag
    15961 tcaccattgg cgttcttcca ggacctgcga atcgaacggg cgcagaacct cgtctcgatt
    16021 ggctacgacc tcgagacaat cgcaagcgag gtcggctacg ccgactcggc gacattgcgg
    16081 agcttattgc gtcggaggct cggccgtggg gtgcgggaac ttcgtgcgga aatgcggtag
    16141 cgtcctgacg acggatttgc ctttacgcga cgcgttgcaa agcggcgaaa acgtggcgta
    16201 cagcaaattc accgcgcaaa cggcgaacgc tcagagttca ggcgctgcgt cccatttggg
    16261 tttcatttct ttaccccatt tgtgcggatc ctgtttgtac atctcttcca tttccctggc
    16321 gacatccgga tgcttgtcca tcacatgttt cgtcatggct ttcaccatct cgtcccatga
    16381 ttcggccgat a
//
LOCUS       DATASH010000701         2393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_905900,
            whole genome shotgun sequence.
ACCESSION   DATASH010000701 DATASH010000000
VERSION     DATASH010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_905900"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="VHV99_18645"
     CDS             complement(<1..673)
                     /locus_tag="VHV99_18645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018432416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3637464.1"
                     /translation="MPTHWMPRAAGICATLIALIVATALPGVALGKDTPEKPTLTMAV
                     GGLPGLYYLPVLAAQQLGYFKDEGLDVTLEDFAGGSKALEAVVGGSADVGAGAFEHTL
                     FMQAKGQRYRAFALMGRAPQIVVAVLKSKADQIKTLADFKGAKVGVSAPGSSTDLVLT
                     VALRKAGVQRNEISVIGVGSGATVLAAVSGGQLDALSNVDPMMTKLLRSGAIKVLVDT
                     RTVKGT"
     gene            complement(932..1285)
                     /locus_tag="VHV99_18650"
     CDS             complement(932..1285)
                     /locus_tag="VHV99_18650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637465.1"
                     /translation="MPPVRLLQSVVAMAVAASLTTVFAVPPAFAASSNNARKPVILDS
                     QSGISDGQSGTVLQTAPFSRQPIVEAQPIAAPAELAPNSSIPVVVAPYIAVPGGGGTP
                     YPQPQPHPRPVPRAQ"
     gene            complement(1515..1661)
                     /locus_tag="VHV99_18655"
     CDS             complement(1515..1661)
                     /locus_tag="VHV99_18655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3563 family protein"
                     /protein_id="HEX3637466.1"
                     /translation="MFAYVLEKLSTWFETAERSRREAYLASSSDIVQLEQRIRSLETD
                     GYSL"
     gene            complement(1956..2378)
                     /gene="cueR"
                     /locus_tag="VHV99_18660"
     CDS             complement(1956..2378)
                     /gene="cueR"
                     /locus_tag="VHV99_18660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006878 - intracellular copper ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HEX3637467.1"
                     /translation="MNIGEAARVSGVTAKMIRYYESVGLLAAKARTSAGYRVYGPQEV
                     HSLRFIRQARRLGFLVEDIRRLLALWHDRSRASAEVKAIALEHVAELDRRIAELTDMR
                     DTLAHLADHCHGDERPDCPIIERLADTDLVSGKGCHPI"
BASE COUNT          476 a          737 c          710 g          470 t
ORIGIN      
        1 gcgtgccctt cacggtacgt gtatcgacca gcactttgat cgcgccgcta cgcagcagtt
       61 tggtcatcat cggatcgaca ttcgacagcg cgtcgagctg accgccgctc accgcagcga
      121 gtaccgtggc accgctgccc acgccgatca ccgaaatctc gttgcgctgc acgccggcct
      181 tgcgcaaagc cacggtgagc accagatccg tcgacgaacc gggcgcgctc acgccgactt
      241 tcgccccctt gaagtccgcc aatgtcttga tctgatccgc cttcgacttc aacacggcga
      301 cgacgatctg cggcgcgcgg cccatcagcg cgaacgcacg gtagcgttgc ccctttgcct
      361 gcatgaacag cgtgtgctcg aatgcgccgg cccccacgtc ggcgctgcca cccacgaccg
      421 cttccagcgc cttcgagccg cctgcaaaat cttctagcgt gacgtcgagc ccttcgtcct
      481 tgaagtaacc caactgctgg gcggcgagca ccggcaaata gtagaggccc ggcaagccgc
      541 cgacggccat cgtcagcgtg ggcttttccg gtgtgtcctt gccgagcgca acgcccggga
      601 gcgccgtggc tacgatgagc gcgatcaacg tggcgcaaat cccggcggca cgcggcatcc
      661 agtgagtagg catttaatgt ctccttgttc tgttttcatt cgatgcgatg gcgcaacgca
      721 tcgtgcatgg cccatatcgc gggcgagaga gcgcgcaacg attgtcgtcg gacgggttct
      781 tacacgccat gcgccgaaac ccgaatggcg tgcagtgtct cgcgtactgg cacaggcttg
      841 cgcgcacatg cggcggcacg accgcggggc cgcggctcga ggcttcaaca gatggagaag
      901 aaactactac gaggtatcgc gcgcaagacg ctcattgcgc gcgcggcacc ggacgagggt
      961 gtggctgcgg ttgtggatac ggcgtgccac cgccgccagg caccgcgatg taaggcgcga
     1021 caacaaccgg tatcgacgaa ttgggcgcca gctccgctgg cgccgcgatg ggctgcgcct
     1081 ctacgatcgg ctgccgcgaa aacggggcgg tctgcagcac ggtgccgctc tggccgtcgc
     1141 ttatcccgct ctgcgagtcg agtatcacgg gcttgcgagc gttattgctc gatgcggcga
     1201 aagccggtgg tacggcgaaa accgttgtca gcgaggcggc gacagccatg gccacaacgg
     1261 attggagcaa gcggacagga ggcatgggcg gaccggttag gtttgaaaat gccggattgc
     1321 acagaaaacg ctttacctta ccgcggggct aaccgtcagg ctagtcgtta ttggccatga
     1381 gtagcgtgaa cgcgccactt gccgtgaaat ttacccggtc cagatgtgac aaagccccgt
     1441 ctatacgggg ctcaacactt tactgcgatg gccgcgcacg agcgctgccg ggtcaaactc
     1501 aatgccattg aaacttacag cgaatagccg tcggtttcga gcgagcggat gcgctgttcg
     1561 agctgaacga tatccgacga cgatgccaga taggcttcac gacggctgcg ttccgcagtt
     1621 tcaaaccagg tgctcaactt ttcaagtacg tatgcaaaca tgatgttctc caaggatcag
     1681 attgaatccc cggtgagttc tgcatcaggg atttcccgta aaagggttaa cccggattat
     1741 aaccctatgg tgcaaccttg ctagtgaaat gcccgcatga cgcgcattcc gttttggaat
     1801 ggtgcacgct tgcgatgcac ttaagtcatt gatttatcta ggtgcggatt ggtttctttg
     1861 agatatccga tgttgcccca aacgaattgg caccatgatg gtgcttttga ttgatcggtt
     1921 gcgacaaacc ttcaaccagg ttgcacccaa caggttcaaa tcggatgaca acccttcccc
     1981 gacaccagat cggtgtccgc gagccgttcg ataatcgggc aatcggggcg ctcgtcaccg
     2041 tggcaatgat cagccaggtg cgccaatgtg tcgcgcatgt cggtcagttc ggcaatgcgg
     2101 cgatcaagtt ccgcgacgtg ttccagcgca atcgccttca cttcggcgct ggcgcgcgag
     2161 cggtcgtgcc acaacgccag caaccggcgg atgtcctcga ccagaaaacc tagacggcgc
     2221 gcctgccgga tgaaacgcag cgaatgaacc tcctgcggcc cgtatacgcg atagccggcg
     2281 ctcgtgcgcg cctttgccgc cagcaggccc acgctttcgt agtagcgaat cattttcgcc
     2341 gtgacgcccg acacgcgggc cgcttcaccg atgttcatga ctgtttcccc agt
//
LOCUS       DATASH010000702         9004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5060660,
            whole genome shotgun sequence.
ACCESSION   DATASH010000702 DATASH010000000
VERSION     DATASH010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 9004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9004
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5060660"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VHV99_18665"
     CDS             <1..244
                     /locus_tag="VHV99_18665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AtzH-like domain-containing protein"
                     /protein_id="HEX3637468.1"
                     /translation="ATENLQGYDAIRQFRAKRPGKGLMRTIVDRSITTFGNDFAVANI
                     TFTRPDVSRIGRQSQTWVKVAEGWRVIAARVSWMDE"
     gene            241..1452
                     /locus_tag="VHV99_18670"
     CDS             241..1452
                     /locus_tag="VHV99_18670"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX3637469.1"
                     /translation="MKRDDLLQPGMHGAFVRDGFGVLPEPVASRHTTLSGYRLAVKDV
                     FDVAQLCTGAGNPAWAEEQLGATTTAVAVRLILEHGAQWLGKTVTDELTYSLAGINVH
                     YGTPRNPADADRVPGGSSSGSAVAVAAGYADIGLGTDCGGSIRLPASYCGIWGMRPTH
                     GRIAVNGCLTLAHSFDTVGWFTRDSALLADVLSVLTHEAPQQPLAPMRLHVPEDVLPL
                     LDPQAASAFNAAIETMRDRLPIESFSAGWPLDQWASAFRVLQGAEIAQQHGLWAAAHI
                     DSFGADIAARMKSAMQITSDAVWAAQRTRVAVIHRLAQVFAQPRTYFLLPTVPCVAPR
                     FDAPADVVDATRARSLQMLCIAGLAGLPQVSMPWTAFDGAPLGLSIIGARGDDSGVAA
                     VARAVHAAMTG"
     gene            1526..3019
                     /locus_tag="VHV99_18675"
     CDS             1526..3019
                     /locus_tag="VHV99_18675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3637470.1"
                     /translation="MSTTHDCAWLLAEPGSLEAQANVRLVVEGGRIAAIVPASAKPPA
                     GPRRLALPALTNAHDHGRPFRSATVDAFEKPLESWLPFMGLIPGADPYLSAATAFAQS
                     VRHGVANLMVHYTRVQGGMSYVDEALAVARAARDVGNRIGFAIALRDRQGIAYCDDAS
                     ALAALRPEIRNAVAARLATKPMPAAQQLALVDEVARMVEEEEYAEHMTVQYGPTGVQW
                     CSTPLLEAIAQRSADHDRPIHMHLLETRYQREWADHTYPQGIVRFLDDLGMLGPRLTL
                     AHCVWARPDELSLLAERGVTIAVNTSSNLYLKSGIAPVPDMLRHGCRIALGLDCTGID
                     EDNDALREMRLAYQLHRGWGFEVTMNRAQLWTFAACNGRRSVNGAAHDASSGGRLAIG
                     EPADLVLLNWDALDNDALLPVDPLDLLLARGSGMHIDEVVIGGRTVVAQGRVCGVDEH
                     ALRAELIAQMRERIAAQPAVSAWQGTLRALDEDLGPFYLKSAWRGCC"
     gene            complement(3112..4635)
                     /locus_tag="VHV99_18680"
     CDS             complement(3112..4635)
                     /locus_tag="VHV99_18680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008346672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3637471.1"
                     /translation="MALQTVGMTKHFGSHVALDDVSIKVSAGSIHALLGENGAGKSTL
                     VKCVMGEYTPTRGQILLDNREVAIDGLRDAHRHGMGMVYQHFTLVPSLTAAENLVMAR
                     ADVPALIDWQAEKARLRTFLDRMPFKLPLDRPVSTFAAGERQKLEILKQLYLARRLLI
                     LDEPTSALTPGEADQVLSLLRELTRTDALTVIMISHKFREVMEYADDVSVLRRGKLAG
                     AGRVSELSVDAMSRMMVGNSVSREAAPRRHWQGGEQPVALEVDGVGADDDDGLPAVRD
                     VSLYVRAGEVLGIAGVSGNGQVELVEVLSGQRQPTRGRLRVHGRPYSPRRAEMERSKV
                     FCLPEEPLRNATVPHMSVAENIAFRVFDQPGVRFFGGWLSPARIRRHARKLIDSYRVK
                     TASPDEPIKNLSGGNVQRAILSRELSADVKLLIVANPCFGLDFASAADIRARIIEQRN
                     RGAAVLLISEDLDEIIELADRIAVMSEGRIVYEAAAHVADQQSIGRAMAGEAQTAPA"
     gene            complement(4790..5677)
                     /locus_tag="VHV99_18685"
     CDS             complement(4790..5677)
                     /locus_tag="VHV99_18685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3637472.1"
                     /translation="MFAGAIRVSTPFLFVSVGECLTEKSGRINLGLEGTLVFGAMAAY
                     AVSFHTHSPWLGTLFAGIAGAAFGALHGAICRLPKVNDVAIGIALMLFGTGLAFFFGK
                     PYIEPEAPHLPSMALGNWSSLPQVRAALQINPLFFIGIALAVLFAWALRRTRWGLIVR
                     MCGDSAAATRSAGYSVDGVRFVVTAIGGFLAGVGGAFLSLSYPGSWSEGLSSGQGMMA
                     VALVIFARWSPLGCLGASLLFGAAAAIGPALQTVGITAGYDIFNAAPYLLTLVFLIAS
                     SSSRRNLKGAPSELSISRR"
     gene            complement(5732..6865)
                     /locus_tag="VHV99_18690"
     CDS             complement(5732..6865)
                     /locus_tag="VHV99_18690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3637473.1"
                     /translation="MASDSTQTIPQPVTADGPSERDARFAQFKQWAELLTISALAVLG
                     AAVVFSLLLLALGASPGEFLALLWRGGFGTWFSLQNTLVRAAPLIFTALAVAIPAQLG
                     LVIIGGEGALVLGGFAAACAALPFHGGGSVPLVMAAMIASALAAGGLWIGIVGALRHF
                     RGVNETISSLLMSYLAIAVMNYFVDGPLRDPASPIKSSTLPIGDANMIGNIPGTSVHW
                     GLVVAVALASASWVLMRRTTFGFAARLTGGNVRAARAQGLPVGRLIITACALAGACAG
                     FAGYFEVAAVQGRANASLAAGYGFSGILVSFLARHNALAIVPVAILVGGISAAGGLIQ
                     RRLDLPDATVLVLQGIVFLFLLVSETLYGRFAMFRPKRSPSDI"
     gene            complement(6940..8037)
                     /locus_tag="VHV99_18695"
     CDS             complement(6940..8037)
                     /locus_tag="VHV99_18695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX3637474.1"
                     /translation="MNRRRFLTLSGGGAMGLAAGMLPRLASAKEKLTIGIVYVGARDD
                     FGWNQSHAVAAQALRGLPNVSVVEEENVAETIAVRKTMESMIQLDGASLILGTSFGYY
                     DPYMVDLARAYPDVQFRHASKLWDPSKAPNNLGGFFAYRDQGHYVNGVAAGLSTKTNK
                     IGYVAAKPIGLVLRTINSFTLGVRKVNPNATVQLIFTGDWSLPVREAEATNALISSGC
                     DVIACHVDSPKVVVQTAEQHGARSCGHNTSQAALAPKGFITGAEAKWETAYKAYANDI
                     AAGRKLPNLIFGGYDKDLVASTPFGAGATEGARKAALQAIADLKAGKPIYVGPIRSNA
                     GKIVIDKTYGNYDPFLEQTSYLVEGVHGSIA"
     gene            complement(8195..>9004)
                     /locus_tag="VHV99_18700"
     CDS             complement(8195..>9004)
                     /locus_tag="VHV99_18700"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX3637475.1"
                     /translation="IIALHTDFTKLFMTSVSRADIAYLSLRQAIVEQALVPGTKLRED
                     VLATHFAVSRTLVRAALARLANEGLIDTGNKRTATVARPSREEAKAVFEVRRCLEAEV
                     TRLVIKNWQPNYTTVLEEHVLKEEESDRAGNPKLSLRVASDFHLLLAQLTGNALLERY
                     VSEVVSRCSLILAVHGHEQSQECGIREHHQLIAALSKRDDVRAQHLMEHHLQSLEERA
                     LAEKPDASRSDITEILGRYSASFSPADKPASSLEGGGAASRGAGTRHAHDK"
BASE COUNT         1798 a         2839 c         2745 g         1622 t
ORIGIN      
        1 cgcgacagaa aatctgcaag gctacgatgc gattcgccaa tttcgggcaa agcgccctgg
       61 caaggggctg atgcgcacga tcgttgatcg atcgatcact acattcggca acgatttcgc
      121 cgtcgcgaac atcacattca cgcgcccgga cgtatcccgc atcgggcgcc agtcgcaaac
      181 ctgggtgaag gtggcagagg gttggagggt gatagccgca cgcgtgagct ggatggacga
      241 atgaaacgag acgatctgtt gcagccaggt atgcacggcg catttgtgag ggacggtttt
      301 ggcgtgttgc cggaacctgt agcgtcccgg cacacgacgt tgtcaggcta tcgccttgca
      361 gtcaaagacg tgttcgacgt ggcacaactt tgtacggggg caggcaaccc cgcatgggcc
      421 gaagaacaat taggggcgac gacaacggcc gttgccgtgc ggctcatcct cgaacacggc
      481 gcccaatggc tcggcaagac agtcactgac gagctgacct acagtcttgc cggaatcaac
      541 gtgcactacg gcacgccgcg taaccctgcc gatgccgacc gtgtgccggg cgggtcgtcg
      601 tccggttcgg ccgttgcagt ggccgccggt tatgccgaca tcggactcgg caccgactgc
      661 ggcggctcga tccggctgcc cgcaagttac tgcggcatct ggggcatgcg ccccacgcat
      721 ggtcgcatcg ccgtgaatgg ttgcctcacg ctcgcacata gcttcgacac ggtggggtgg
      781 ttcacgcgcg acagcgcgct gctcgccgac gtcttgagcg tgctgacgca tgaagcgcca
      841 cagcaaccgc tcgcaccgat gcggttgcac gtgcctgaag acgtcctccc gctgcttgat
      901 ccgcaggcgg caagcgcatt caacgcagcc attgaaacca tgcgtgaccg cctcccgatc
      961 gagtcgttct ccgccggttg gccgcttgat caatgggcta gcgcatttcg cgttctgcaa
     1021 ggcgcggaaa tcgcgcaaca acacgggctt tgggctgcgg cacatatcga tagcttcggc
     1081 gcggacatcg cagcgcgcat gaagtccgcg atgcagatca cgagcgatgc agtgtgggcc
     1141 gcgcagcgca cgcgagtcgc ggtgatccac cggctcgcgc aagtcttcgc gcaaccgcgc
     1201 acgtattttc tgctgccgac ggtaccttgc gtcgcgccgc gcttcgatgc gccggccgac
     1261 gtggtagacg cgacgcgcgc acgttcgctg cagatgcttt gcatcgctgg cctcgcgggc
     1321 ttgcctcaag tcagcatgcc gtggacggca ttcgacggcg caccgcttgg cctatcgatc
     1381 atcggcgcgc gcggcgacga cagcggcgtg gctgccgttg cgcgtgccgt gcacgcggcg
     1441 atgacgggat gatcggttcg gcttttgtac cattccgacg tcgaatcgcc aacccatcca
     1501 cgaagcattc attcagatca gtaccatgag caccacccac gactgcgcat ggctcctggc
     1561 cgagcctgga tctctggaag cgcaagccaa cgtgcgcctg gtggtcgagg gcggccgcat
     1621 tgcagccatc gttcccgcgt cggctaagcc gcccgccggc cctcgtcgtt tggccttgcc
     1681 cgcgttgaca aacgcgcacg atcacggtcg gccctttcgt agtgcgaccg tcgatgcatt
     1741 cgaaaagccg cttgagagct ggctgccttt catgggactc atccccggcg cggatcccta
     1801 cctgagcgca gcgacggcgt ttgcccagtc ggtgcgccat ggtgtcgcca acctgatggt
     1861 ccattacacg cgggtgcaag gcggcatgtc ctacgtcgac gaagccctgg ctgtggcccg
     1921 ggctgcgcgc gacgtcggta atcgaatcgg cttcgctatc gcgctacgcg accgccaagg
     1981 tatcgcttac tgtgacgatg cgtcggcgtt ggctgcgctt cgcccggaaa tccggaacgc
     2041 ggtcgcggca cgcctcgcca ccaagcccat gcctgcggcg cagcaactcg cacttgttga
     2101 tgaggtcgcc cgcatggtcg aggaggaaga gtacgccgaa catatgacgg tgcaatacgg
     2161 gcccaccggc gtgcaatggt gttcgacgcc gctgctcgag gcgatcgcgc agcgatcggc
     2221 cgatcacgac cggccgatcc acatgcattt gctcgagacg cgctatcagc gcgaatgggc
     2281 tgatcacacc tatccgcaag gaatcgtcag atttctcgac gacctcggca tgctcggtcc
     2341 aaggctgaca ctggcacatt gcgtgtgggc tcgacccgac gagctgtcgc tgctagccga
     2401 gcgcggcgtg acgattgccg tgaatacgag ctccaatctg tacctgaagt ccggcatcgc
     2461 gccggtgcct gacatgctgc ggcacgggtg ccgcattgcg ctaggcctgg attgcactgg
     2521 catcgacgaa gacaacgacg cgctgcggga aatgcgtctg gcgtaccaat tgcaccgcgg
     2581 ttggggcttc gaggtgacca tgaaccgcgc acaactgtgg accttcgcgg catgcaacgg
     2641 ccgccgaagc gttaatgggg cggcccatga tgcatcctcg ggcggccgcc tggccatcgg
     2701 cgaacccgca gacctagtcc tgctgaactg ggacgcgctc gacaatgatg ccctgttgcc
     2761 agtcgatccg ctcgatctgc tgctcgcgag aggcagcggt atgcacatcg acgaggtcgt
     2821 catcggcggc aggaccgtgg tggcccaagg ccgggtgtgc ggtgtcgacg aacacgcgct
     2881 gcgtgcggaa ctgatcgcgc agatgcgtga gcgcatcgcc gcgcagccgg cagtcagtgc
     2941 atggcagggc acgctgcgcg cgctggacga agatctgggg ccgttctatc tcaagagcgc
     3001 atggaggggc tgctgctgat ccgccgcttt aacgggcggc cgaaccggta ggggccgctc
     3061 caaccggggt aacatcgaaa gccgcccgaa gtgcagcgcg cctgtaatcg atcaggccgg
     3121 cgccgtctgc gcttcgcccg ccatcgcccg cccgatcgat tgctggtcgg caacatgcgc
     3181 agcggcctca taaacgatgc gtccctcact catcactgca atgcgatcgg cgagttcaat
     3241 gatttcgtcg aggtcttctg aaatcagcaa cacggcggcg ccgcgattgc gctgctcgat
     3301 gatccgtgcg cggatatcgg ctgccgatgc aaagtcgagg ccgaagcatg gattggcgac
     3361 gatcaacagc ttgacgtcgg cggacagctc acgagaaaga atcgcgcgct gcacgttacc
     3421 gcccgacagg ttcttgatgg gttcgtcggg cgacgcggtc ttcacccggt aggaatcgat
     3481 cagcttgcgc gcgtgtcgac ggatacgcgc aggcgaaagc cagccgccga aaaaacgtac
     3541 acccggctgg tcgaacacac gaaatgcaat gttctcggcc accgacatat gcggcaccgt
     3601 ggcattgcgc agcggctctt cgggcaggca gaacaccttg ctgcgctcca tctcggcgcg
     3661 ccgcggcgaa tacgggcgcc cgtgcacgcg aagccgcccg cgtgtcggct gccgctggcc
     3721 ggacagcact tcgacaagtt cgacctgacc gttgcccgat accccagcaa tgccgagcac
     3781 ttcgccagcg cgcacgtaga ggctgacgtc acgcacggcc ggcaagccgt cgtcgtcgtc
     3841 ggcgcctacg ccgtcgacct ccaatgcgac gggttgctcg ccgccctgcc agtgccggcg
     3901 gggcgccgct tctcgcgaca cagagtttcc aaccatcatc cggctcatcg catcgacgga
     3961 cagctcgctc acgcggccgg cgccggctag cttgccgcgc cttagcacgc tcacgtcgtc
     4021 ggcatactcc atcacttcgc gaaacttgtg gctgatcatg atgaccgtca gggcgtcggt
     4081 gcgggtcagc tcgcgcagca gcgagagtac ttgatcggct tcccccggcg tgagcgccga
     4141 cgttggctcg tccagaatga gcaggcggcg ggcaagatag agttgcttaa gtatttcaag
     4201 cttttggcgc tctccagctg caaaggttga caccggccga tccagcggca atttgaacgg
     4261 catgcgatcg aggaaggtgc gcagtctcgc tttctcggct tgccagtcga tcagcgcggg
     4321 gacgtccgcc cgcgccatca cgagattctc ggcggcggtg agcgacggga cgagcgtgaa
     4381 gtgctgataa accatgccca tgccatggcg atgcgcatcg cgtagtccgt cgatcgcgac
     4441 ctcccgattg tcgaggagga tctgcccgcg agtcggcgtg tactcgccca tcacgcactt
     4501 gacgagcgtc gatttgcccg cgccgttttc gccgagcaac gcgtgaatcg agccggcact
     4561 tacctttatc gaaacgtcgt caagtgcgac gtgcgaaccg aaatgcttcg tcatgccgac
     4621 ggtttggagc gccaatccac cctcccgctt tttgtcgcga gtgtgcggag cgccgggtac
     4681 agtcgattgt agagtcatgg atcaacgctc taaagcaaac aaatcactaa actattcgaa
     4741 caaacgtcgc tgcggcatct ttcacgacga tgctgcgatg tcccgtatat cagcggcgcg
     4801 agatcgacaa ctcgctcggt gcccctttca ggttgcggcg cgacgaggac gatgcgatca
     4861 aaaacacgag cgtcaacaaa tagggggctg cattgaagat gtcgtagcct gcggtaatac
     4921 cgacggtctg cagtgccggt ccgatcgctg ccgccgcgcc gaaaagcaat gatgcaccga
     4981 ggcaccccag cggactccag cgtgcaaaaa tgacgagcgc caccgccatc attccctgcc
     5041 cggacgaaag gccttcgctc caactgccag gatacgacag ggacaggaac gcgccgccca
     5101 cgcctgccaa aaagccgccg attgcagtaa cgacgaaccg cacaccatcc accgaataac
     5161 cggccgagcg cgtagcagct gccgagtcgc cacacatgcg cacgatcaag ccccagcggg
     5221 tgcgtcgcaa ggcccaggca aacagcaccg cgagggcaat cccgatgaag aaaagcggat
     5281 tgatttgcaa cgccgccctc acctgtggca acgaagacca attgccgagc gccatcgacg
     5341 gcagatgcgg cgcctccggt tcgatgtacg gcttgccgaa aaagaatgcg agaccagtgc
     5401 cgaacagcat gagtgcgatg ccgatcgcaa cgtcgttaac ctttggcaat cgacagatgg
     5461 cgccatgcag cgcgccgaac gcggcgccgg caataccggc gaacaatgtt cccagccacg
     5521 gcgagtgcgt atgaaagctc accgcatagg cggccattgc accgaacacg agcgtaccct
     5581 cgagtccgag gttgatccga ccggactttt cggtcaggca ttcgccaaca cttacaaaaa
     5641 gaaacggcgt cgagacacgg atcgcgccgg cgaacatcgc gagcggaacg gtccaaaggc
     5701 caatatcggt aatcgccata gagagtgctc tttaaatgtc ggacgggctg cgcttcgggc
     5761 gaaacatcgc aaagcgcccg tacaaagtct cactgaccag caggaaaagg aagacgatgc
     5821 cctgcagcac cagcacggtc gcgtcgggca agtcgagccg gcgctgtatc aagccgcccg
     5881 cagcagatat gccgccgacg agaatcgcca ccggcacaat ggcgagcgca ttgtgccggg
     5941 caagaaacga cacgaggatg ccggagaagc cataacccgc ggcgagcgac gcgttggcgc
     6001 gcccctgcac tgctgcgacc tcgaaataac cggcaaaccc tgcgcaagca ccagcgagtg
     6061 cacaggcggt aataatcagc cggcccacgg gcaatccttg cgcgcgcgct gcgcggacgt
     6121 tgccgcccgt caggcgtgcg gcgaagccga aggtcgtccg tcgcatcaat acccaggagg
     6181 ccgacgcgag cgcgacggca acgaccaatc cccagtgcac ggatgtgccc ggaatgttgc
     6241 cgatcatatt tgcgtcgccg atcggcaaag tcgacgattt gattgggctt gcagggtcgc
     6301 gcaacggacc atcgacgaag tagttcatga ctgcgatcgc gagatacgac atgagcaaac
     6361 tggagatggt ctcgttgact ccgcgaaagt gccgcaaagc gccaacgatg ccgatccaca
     6421 gaccgcctgc ggcgagtgca gacgcgatca tcgctgccat tactaacggc actgaaccgc
     6481 cgccatgaaa tggaagtgcc gcgcaggcgg cggcgaaccc gccgagcacg agcgcccctt
     6541 caccgccgat gatcacgagt ccgagttgtg ccggtattgc gacggcgagc gccgtaaaga
     6601 ttaacggagc tgcgcgcacc agcgtgtttt gcagtgaaaa ccaagtaccg aaaccgccgc
     6661 gccaaagcag tgcgagaaac tcgcccggtg aagcaccaag ggcaagcaac aacaaggaaa
     6721 aaaccaccgc ggcaccgagc acggcgagtg cggagatggt cagcaactct gcccactgct
     6781 tgaattgcgc gaaccgtgcg tcacgttccg aggggccgtc agccgtgacc ggctgcggga
     6841 tggtttgcgt cgagtcgctc gccataaaat ctcttgagaa aaagaaaagc ccggctggcc
     6901 ggccctgatt catcggggcc ggtcgccgtg atgcagcact taggcaatcg aaccgtgcac
     6961 gccctcaacc agatatgagg tctgttcgag gaatggatcg tagttgccgt aggttttgtc
     7021 gatcacgatc ttgccggcgt tgctgcggat gggaccgacg tagatcggct tgcctgcctt
     7081 caggtcggca atcgcctgaa gcgcggcctt acgtgcacct tcggtggcac ccgcgccgaa
     7141 cggcgtgctc gcgacgaggt ctttgtcgta gccgccgaag atcaggttcg gcaatttgcg
     7201 tccggcggcg atatcgttcg cgtaggcctt ataagcagtt tcccacttcg cttcagcacc
     7261 cgtgataaaa cccttcggcg ccagcgcagc ttgcgatgtg ttgtggccgc agcttcttgc
     7321 gccatgctgc tcggcagttt ggacgaccac cttcgggctg tcgacatggc aagcgatgac
     7381 gtcacaaccg ctcgagatta acgcgttggt cgcctctgcc tcgcgcaccg gtagactcca
     7441 gtcacccgtg aagatgagtt gaaccgttgc gttcggattc accttgcgca caccgagcgt
     7501 aaacgagttg atcgtacgca gcaccagccc gatcggcttc gccgccacgt agccgatctt
     7561 gttggtcttc gtcgaaaggc cggcggcgac accgttgaca tagtgcccct ggtcccgata
     7621 cgcgaagaat ccgcccaggt tattcggggc cttgctagga tcccaaagct tgctggcgtg
     7681 gcggaattgg acatccggat aggcacgcgc cagatcgacc atgtacggat cgtaataccc
     7741 gaacgaggta ccgagaatca gcgaggcacc gtcgagctga atcatggact ccatggtctt
     7801 cctgaccgcg atcgtctcgg ccacgttttc ttcttcgacc acgcttacgt tcggcagccc
     7861 gcgcaaagcc tgcgcggcca cggcatgcga ctggttccag ccgaagtcgt cgcgcgcacc
     7921 gacatagaca atgccgatcg tcaacttttc tttcgcgctc gcaagccgcg gcaacattcc
     7981 agccgcgaga cccatcgccc cgcccccgga tagggtcaaa aacctgcgtc gattcatttg
     8041 cttttccatc ttgttgctcc gctcgtcatt cgtcactctt gttgcaattt cggtatcact
     8101 acaccgtcaa atccgggtgt ccgggaagtc tcacccagca caaatctcgt gttctttcat
     8161 ttccagtcgc ctgatgcatc gacatccatg cccatcactt gtcgtgcgcg tgcctggtcc
     8221 ctgcgccgcg tgaagcggcg cccccgcctt cgagagaaga cgccggcttg tcggccggcg
     8281 agaagctcgc ggagtaacgg ccgagtattt ccgtgatgtc ggaacgcgaa gcgtcaggtt
     8341 tttccgcaag tgctcgttcc tcgagcgact gcagatgatg ctccatcaga tgctgcgcgc
     8401 ggacatcgtc gcgcttgctc agcgcggcga tgagctgatg gtgctcgcga ataccgcatt
     8461 cctgcgattg ctcgtgaccg tgcaccgcca aaatcagcga gcaccgcgat acaacttcag
     8521 aaacgtatcg ctccagaagc gcgttgccgg tcagttgcgc aagcagcaaa tggaagtcgc
     8581 tcgcgactcg cagcgataac ttcggattgc cggccctgtc ggactcctcc tccttgagca
     8641 catgctcttc gagaacggtc gtgtagttcg gctgccagtt ttttatgacg agacgcgtga
     8701 cctcggcttc aagacagcgg cgcacttcga acaccgcctt cgcttcttcc cgacttggac
     8761 gagcgacggt cgctgtacgt ttattgccgg tgtcgatgag gccttcgttg gcaagccgcg
     8821 caagcgcagc gcggaccagc gtgcgactga cagcgaaatg cgtggcgagg acgtcttcgc
     8881 gcaacttcgt gccgggcacg agcgcctgtt caacgatggc ctgtcgcaac gacagatagg
     8941 caatatcagc acgactgact gaggtcatga aaagcttggt gaaatccgta tgtaaggcaa
     9001 tgat
//
LOCUS       DATASH010000703         4242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2686599,
            whole genome shotgun sequence.
ACCESSION   DATASH010000703 DATASH010000000
VERSION     DATASH010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2686599"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..>4242
                     /locus_tag="VHV99_18705"
     CDS             1..>4242
                     /locus_tag="VHV99_18705"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012404525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX3637476.1"
                     /translation="MTAAKLSTSNPLDGFHPAVAAWFTRHFAAPTEAQKRAWPLIKAR
                     SSTLVAAPTGSGKTLTAFLAAIDELVREALASNKPLPNETTVVYVSPLKALSNDIRVN
                     LQEPLAGISTELEQLGLPPVEIRAAVRTGDTTPQERNAVKKRPPHILVTTPESLYVLL
                     SSDSGRAMLSTVRTVIVDEIHAVAGNKRGAHLALSLERLDALCVEHQHEAPVRIGLSA
                     TQKPIELVAQFLTGNRACSIVDVGHVRKRDLALELPPIPLEAIMSNDVRERVYDRLAD
                     LIAQHRTTLVFVNTRRMAERVARHLTERLGKPAVAAHHGSLAKEQRLDAEQRLKRGEL
                     QVLIATASLELGIDIGDVELVCQLGSPGSIGGFLQRAGRSGHQVSGTPKGRLFPESRD
                     DLIECAALLDCVARGELDVLHIPQAPLDVLAQQIAAEVSCREWNEDALFELVRSAMPY
                     ASVDRTQYDAVLRMLAEGYTSRRGVRGAYVHRDAVTRSLRGRRGGRLTAVTSGGAIPD
                     NADFAVLLEPQGLQIGTVNEDFAVESLAGDVFQLGNASYKILRVEGGRVRVEDAHGQP
                     PNIPFWLGEAPGRSNELSAAIARLHAQADAWLGEFSLDITITKLADQTGIDEDAARQI
                     VDYLARSRSALSVLPTQDVLVMERFFDASGGTQLVIHAPFGSRVNRAWGLALRKRFCR
                     TFNFELQAAATEDAIILSLTGSHSFPLDEVWRYLHSNTAEHMLIQALLDAPLFGVRWR
                     WNATNALALPRYAGGRRVAPQLQRMKSEDLLATVFPDQVACIENVVGERDVPRHPLVD
                     QTIDDCLHDAMDCDAWLALLRRIESGDVRLVTRDLPAPSPLAAEVLSARPYAFLDDAP
                     IEERRTQAVLARGWTDPQSSDDLGALDAVAIEGVREEAWPTVRNADEMHEALSGLSCI
                     TEAESHANDGWPAWLQQLAASGRATRLQVTSADGLWVPVERLTCLRAVHPVAPMQPPL
                     KPLPGFDEAWNEDDALVEIVRARLSGFGPLPAPVIARALAVPVSSVALALTRLEAEGY
                     VMRGRFTPGTKEATAATDEEWCERHLLARIHRYTVRRLRREIEPVELQDFMRFLFDWQ
                     HVSADAKADPHGDGHDALMLAIEQLEGYEAPAVAWEDEILPARLSAYSMTWLDDLCRS
                     GKVVWSRPGARSRSSGGPVRTTPIVLLPRRNLAAWNPLMQSVQSPEPSSRAQLVFDAL
                     AQHGAVFFDELRSDVRLLESELETALGELVSLGLVNADSFAGLRALLTPAARRNSFGR
                     RPRRGGRGHFIGGMDDAGRWALLRRSESSAAPGTDSIEHVAMTLLRRYGVIFWRLLER
                     EADWLPPWRDLLRVFHRLEARGEIRGGRFVAGLAGEQFALPEAVPLLRTMRNRPADGS
                     FIAISAVDPLNLAGTLLPGEKVPALPGNRVLYCDGL"
BASE COUNT          720 a         1312 c         1413 g          797 t
ORIGIN      
        1 atgaccgctg caaaactatc gacttccaat ccgctcgacg gcttccatcc ggctgtcgcg
       61 gcctggttca cccggcattt cgccgcgccc acggaggcgc agaagcgcgc ctggccgctg
      121 atcaaggccc gcagttcgac actcgtggcc gcgccaaccg gctcgggcaa gacgctgacg
      181 gcattccttg cagcaatcga tgaactggtt cgggaagcgc tggcgagtaa caagccgctg
      241 cccaacgaga cgaccgtggt ctacgtctcg ccactcaagg cgctttccaa cgatattcgc
      301 gtgaacctgc aggaaccgct tgccggcatc tcgaccgagc tggaacagct gggcctgccg
      361 cccgtcgaga ttcgcgcagc cgtgcgcacc ggcgacacaa cgccccagga gcgcaatgcg
      421 gtaaaaaagc gcccgccgca tatcctcgtc accacgccgg aatcgctcta cgtgctgctg
      481 agttccgatt ccggccgcgc gatgttgtcg acggtgcgta cagtaatcgt cgatgaaatc
      541 catgccgtcg ccggcaacaa acgcggcgct catctcgcgc tcagtctcga acgcctggac
      601 gcgctgtgcg ttgaacatca acatgaagcg ccggtgcgga tcgggttatc ggcgacgcaa
      661 aagccgatcg aactggtcgc gcaattttta acgggcaatc gagcgtgctc gatcgtcgat
      721 gtcggtcatg tgaggaagcg cgatctcgcg ctcgaactcc cgcccatccc gcttgaagcc
      781 atcatgtcga acgatgtgcg cgagcgtgtg tatgaccggc tcgccgatct catcgcgcag
      841 caccgcacca cgctcgtgtt cgtgaacacg cgccggatgg ccgagcgggt cgcgcgccac
      901 cttaccgagc ggcttggcaa gccagcggtc gcggcgcatc acggcagtct cgcgaaagag
      961 cagcggctgg acgcggagca gcggcttaaa cgcggcgagc tgcaagtgct gattgcaacg
     1021 gcttcgctgg aactggggat tgatatcggc gatgtggagc tcgtctgtca actcggttca
     1081 ccgggttcga tcggcggttt cctgcagcgt gccgggcgct cggggcatca ggtaagcggt
     1141 acgccgaaag ggcggctctt tccagagtcg cgcgacgatc tcatcgaatg cgcggcgctg
     1201 ctcgattgcg tggcacgcgg tgaactcgac gtgctgcata ttccccaggc gccgctcgat
     1261 gtcctcgcac agcagatcgc cgccgaagtg tcgtgccgtg agtggaacga ggacgcgctc
     1321 ttcgaactcg tgcgtagtgc gatgccctat gcatcggttg atcgtacgca gtacgacgca
     1381 gttctgcgga tgctcgccga gggttatacg agccggcgcg gtgtgcgcgg ggcttacgtg
     1441 catcgggatg cggtgactcg cagcttgcgt ggacggcgtg gtggcaggct gacggcagtg
     1501 acatcgggtg gcgcgattcc ggataacgcc gacttcgccg tgctgctgga accgcaaggc
     1561 ttgcagattg gcacggtcaa cgaggatttc gcggtcgaaa gcctggccgg cgacgtgttc
     1621 caactcggca atgcgtcgta caagatcctg cgtgtggagg gtggacgagt gcgggtggaa
     1681 gacgcgcacg gccagccgcc gaacattccg ttctggctcg gcgaagcgcc cggacggagc
     1741 aacgaattgt cggcggcgat cgcgcgtttg cacgcgcaag cagatgcgtg gctgggcgaa
     1801 ttctcactcg acatcacgat caccaagctg gctgatcaga ccggtattga cgaagatgcg
     1861 gcgcgacaga tcgtggatta tctggcgcgc tcgcgatctg cgctgtccgt tctcccgacg
     1921 caggacgtgc tggtcatgga acgtttcttc gatgcctccg gcggcacgca actcgtgatt
     1981 cacgcgccgt tcggcagccg cgtgaaccgc gcgtggggcc tcgcgttgcg caagcgtttt
     2041 tgccgcacgt tcaacttcga attacaggct gcggcgaccg aggacgcgat catcctgtcg
     2101 ctgacgggaa gccacagttt tccgctcgac gaagtctggc gttatctcca ttccaatacc
     2161 gcggaacaca tgctgatcca ggcgctgctc gacgcgccgc tgttcggcgt gcgctggcgc
     2221 tggaacgcga ccaacgcgct cgcgttgccg cgctacgcag gaggccgtcg ggtcgcgccg
     2281 caattgcagc gcatgaagag cgaggacctg ctcgcaacgg tgtttcccga tcaagtggcg
     2341 tgcatcgaaa acgtggtcgg cgagcgtgac gtgccgcgtc atccgctggt cgaccagacg
     2401 atcgatgatt gcctgcacga tgcgatggat tgcgatgcgt ggctcgcgct gctaaggcgc
     2461 atcgagagtg gcgacgtacg gctcgtcacg cgtgatttgc ctgcgccgtc accgttggcg
     2521 gcggaggttt tatcagcgcg tccttacgcc tttctcgacg atgcccctat cgaggaacgc
     2581 cgtacccagg ccgtgctcgc gcgcggctgg accgatccgc aatcgagcga cgacctgggc
     2641 gcgctggacg ccgtggcaat cgagggcgtg cgcgaagaag cgtggccaac ggtgcgcaac
     2701 gccgatgaaa tgcacgaggc gctgtcgggg ctttcatgca tcactgaagc cgagtctcac
     2761 gcgaacgatg gctggccggc gtggcttcaa cagctcgcgg cgtcggggcg agcgacgcgt
     2821 ttgcaggtga cctcggcgga tggattatgg gtgccggtcg agcgcctgac atgcttgcgc
     2881 gctgttcatc cggttgcgcc gatgcagccg ccgctgaagc cgcttcccgg tttcgacgaa
     2941 gcgtggaacg aagacgatgc gctcgtcgag atcgtgcgcg cgcgtctgag cggctttggt
     3001 cctctgcccg cgcctgttat tgctcgcgct ttggcggtgc cggtttcgtc ggtagcgctc
     3061 gcattgaccc gccttgaagc ggaaggttac gtgatgcgag gccgcttcac gccgggcacg
     3121 aaggaggcaa cggctgcaac cgacgaagag tggtgcgaac ggcatctgct ggcgcgtatt
     3181 catcgataca ccgtgcggcg tttgcggcgc gaaatcgagc cggtcgagtt gcaggatttc
     3241 atgcgtttcc tgttcgactg gcaacacgtc agcgcggatg caaaagccga tccgcatggc
     3301 gacggtcacg acgcgctgat gctcgctatc gaacaactcg aaggctacga agcgcccgcg
     3361 gtcgcgtggg aggacgagat cctgcccgca cgcctgagcg cttattccat gacctggctc
     3421 gacgatctat gccgctccgg caaggttgtc tggagccgtc cgggcgctcg ttcgcgcagt
     3481 tcgggcggtc cggtgcgcac cacgccaatc gtgctgctgc cgcgccgcaa cctggcggcg
     3541 tggaatccgc tgatgcaatc ggtgcaatca cccgaaccgt catcgcgtgc gcaacttgtc
     3601 ttcgacgcgc tcgcgcaaca cggcgccgtg ttcttcgatg aactgcgttc agacgttcgc
     3661 ctgctggagt ccgaactcga aaccgcgctc ggcgaactgg tgtcgctggg actggtgaac
     3721 gccgatagct tcgccggcct gcgtgcgttg ctcacgccgg ccgcgcgccg caacagtttt
     3781 ggccggcggc cacggcgcgg cgggcggggc catttcattg gcgggatgga cgacgccggc
     3841 cgttgggcgt tgctgcgccg ctctgaaagc agcgccgcgc ccggcaccga ttcgatcgaa
     3901 cacgtagcga tgaccctgtt gcgtcgatac ggcgtgatct tttggcgctt gctagaacgc
     3961 gaggccgact ggctgccgcc gtggcgtgac ttgctgcgag tctttcatcg gctggaagca
     4021 cgcggtgaaa ttcgcggcgg ccgattcgtg gcgggtctgg caggcgagca gttcgcgttg
     4081 cccgaagccg tgccgctttt acgcacgatg cgcaaccggc ccgccgacgg cagcttcatc
     4141 gcgataagcg cggtcgatcc cttgaatctc gccgggacct tgctgcccgg ggagaaagtg
     4201 ccggcgctgc cgggtaatcg cgtgttgtat tgcgatggct tg
//
LOCUS       DATASH010000704         3742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1499678,
            whole genome shotgun sequence.
ACCESSION   DATASH010000704 DATASH010000000
VERSION     DATASH010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3742
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1499678"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            629..>3742
                     /locus_tag="VHV99_18710"
     CDS             629..>3742
                     /locus_tag="VHV99_18710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004543744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3683 domain-containing protein"
                     /protein_id="HEX3637477.1"
                     /translation="MNAPQVFDPHGAATAVAADPEARLREIPYNYTSFSDREIVIRLL
                     GDDAWAALAELRAERRTGRSARMLYEVLGDIWVVRRNPYLQDDLLDNPKRRALLIEAL
                     HHRLTEIEKRRRADLVEHGDEAGVDRAARVETLVAAARRAVDDFESEFQKTFDLRRRA
                     NKVLGKVTEKDNIKFDGLSRVSHVTDATDWRVEYPFVVLTPDTEAEIAGMIKACFELG
                     LTVIPRGGGTGYTGGAVPLTPFSAVINTEKLEQLGAVEMTELPGVERKVATIFSGAGV
                     VTRRVTEAAEQAGFVFAVDPTSLDASCVGGNVAMNAGGKKAVLWGTALDNLAWWRMVD
                     PEGNWLEVTRLDHNLGKIHDIEVARFELKWFDGSHAPGEKLLRTEALDIKGRVFRKEG
                     LGKDVTDKFLAGLPGVQKEGCDGLITSARWVLHKMPAHTRTVCLEFFGQAREAIPSIV
                     EIKDYLFETSRQGGAILAGLEHLDERYLRAVGYATKSKRNAFPKMVLIGDIVGDDADA
                     VAQATSEVVRMANGKSGEGFVAVNAEARKRFWLDRSRTAAIAKHTNAFKINEDVVIPL
                     DRMGEYTDGIERINIELSIKNKLQLVDALEAFFKGGKLPLGKSDDANEIPSAELLEDR
                     VQQALDLLKKVRTRWEFLRDKLDLSLREAQHYLVGLGYEGLAEKFADRVDAQPDATVF
                     HITQDRTVRVSWKAEIRAELRQIFNGGEFKPILEEAQAIHKKVLRGRVFVALHMHAGD
                     GNVHTNLPVNSDNYEMLQDAHTAVARIMKLARSLDGVISGEHGIGITKLEFLTDEEIG
                     EFRAYKQRVDPHGRFNAGKLLEGADLRNAYTPSFGLMGYESLIMQQSDIGAISESIKD
                     CLRCGKCKPVCATHVPRANLLYSPRNKILATSLLVEAFMYEEQTRRGVSIKHWDEFND
                     VADHCTVCHKCVTPCPVKIDFGDVTMNMRNLLRKMGKKKFNAGSAAGMFFLNATNPST
                     INAARTAMMGIGYKAQRLGNDVLKKFAKKQTAHPPATVGKPPVTQQVIHFMNKKMPGN
                     LPKKTARA"
BASE COUNT          749 a         1163 c         1209 g          621 t
ORIGIN      
        1 cgcagccgtg ccgacagatt cggcgcgcgt tcgagttcgg tctcgcgggc cacgtcgtag
       61 acgcgcgcgg tcagggtttt cttcagatag tcgtgggaag ccatgcgggt ggcgcggtgc
      121 gctttgtgag acgggaaagg gtcaatgata gcgccaacgg tgtgcgctct ggccttcggc
      181 aaaccgtcaa cgaaccttcc acgtacgccc gagggccgcc ggcaaaggcc cgcccggccg
      241 ttcggtcgga gaattactcg gatgcgcagg cggacctcgt tcagcgcaaa aacgctcaaa
      301 tcctcgccac cgacccgatt tcaggcgtga acgcgcggct ggatcatggg gtagaattcc
      361 attttggaat aaggatcgga agatgcactg gcagcctcgg atcgcccatt tgatgcccgt
      421 cccgcgtgag tcttgtcccg cggcggagcg tcacgtccgt caggcacgat cgtgtagcgc
      481 tctctgagcg ccgtgaagcg atcgatgtgg gtagaccgtc ggttgcgctt cgctccccta
      541 gaagatttcc agcattgcgc gccacgcgca ccgtcagtca gcgcctcacc gatgagcgcg
      601 cctggttgac ccaccgagtc gtccgaacat gaacgcacct caagttttcg atccgcacgg
      661 ggccgccact gcggtggccg ccgatcctga agcgcgtctg cgcgaaattc cctataacta
      721 cacgtcattt tccgaccggg aaatcgtcat ccgtctgctc ggcgacgacg cctgggcggc
      781 tttggccgaa ctgcgcgcgg aacgccgcac cggccgctcg gcgcggatgc tgtacgaagt
      841 gctcggcgac atctgggtcg tgcgccgcaa tccttacctg caagacgacc tgctcgacaa
      901 tccgaagcgc cgcgcgctgc tgatcgaggc gctgcaccac cgtctgaccg agatcgaaaa
      961 gcgccgccgc gccgacctgg tcgagcatgg cgatgaagcg ggcgtcgatc gggccgctcg
     1021 cgttgaaacg ctggtcgcgg ctgcgcgccg tgccgtcgac gatttcgaaa gcgagttcca
     1081 gaagaccttc gatttgcgcc gccgcgcgaa caaggtgctg ggcaaggtta cggaaaaaga
     1141 caacatcaag ttcgacggct tgtcccgcgt ctcgcacgtg accgacgcga ccgactggcg
     1201 cgtcgaatac ccgttcgtcg tgctgacgcc ggataccgaa gccgagatcg ccggcatgat
     1261 caaggcgtgt ttcgagctcg gtctgaccgt gattccgcgt ggaggcggca cgggctacac
     1321 gggcggcgca gtgccgctca cgccgttctc ggccgtcatc aacaccgaaa agctcgaaca
     1381 acttggtgcg gtcgagatga ccgaactgcc gggtgttgag cgcaaggtgg caacgatttt
     1441 ctccggtgcg ggcgtggtca cacgccgcgt gaccgaagcg gccgagcagg ccggcttcgt
     1501 gttcgcggtc gatccgacct cgctggatgc atcctgcgta ggcggcaacg tcgcgatgaa
     1561 cgcgggcggc aagaaagcgg tgttgtgggg gacggcgctc gacaacctcg cctggtggcg
     1621 catggtcgac ccggaaggga actggctcga agtcacgcgt ctggaccaca acctcggcaa
     1681 gattcacgac atcgaagtcg cgcgtttcga gctgaagtgg ttcgacggca gccacgcgcc
     1741 gggcgaaaag ctgctgcgca cggaggcgct cgacatcaag ggccgcgtgt tccgtaagga
     1801 ggggctgggc aaggacgtca ccgacaaatt cctcgccggc ctgccgggcg tgcagaagga
     1861 aggttgcgac ggcctgatca cgtcggcacg ctgggtgctg cacaagatgc cggcgcatac
     1921 gcgcaccgtc tgcctcgaat ttttcggcca ggcgcgcgaa gcgattccga gcatcgtcga
     1981 aatcaaggac tatctgttcg aaacgtcaag gcagggcggt gcgattctcg ccggcctcga
     2041 gcacctggat gagcgctatc tgcgcgcggt cggttatgcg accaagtcca agcgcaacgc
     2101 gtttccgaag atggtgctga tcggcgacat cgtcggcgac gatgcggacg cggtggcgca
     2161 agccacctca gaagtggtgc gcatggccaa cggcaagagc ggcgaaggct tcgtcgcggt
     2221 caacgccgaa gcgcgcaagc gtttctggct cgaccgcagc cgcaccgccg cgatcgcgaa
     2281 gcacaccaac gcgttcaaga tcaacgaaga cgtggtgatc ccgctcgacc gcatgggcga
     2341 gtacaccgac gggatcgagc gcatcaacat cgaactgtcg atcaagaaca agctgcaact
     2401 ggtggacgcg ctcgaagcgt tcttcaaggg cggcaagctg ccgctcggca agagcgacga
     2461 cgccaatgaa atcccgagcg ccgagctgct cgaagatcgc gtgcaacagg cactcgatct
     2521 gctgaagaag gtacgcacgc gctgggaatt cctgcgcgac aagctcgatc tgtccctgcg
     2581 cgaggcgcag cattatctgg tcggcctcgg ctacgaaggg ctggcggaga agttcgccga
     2641 tcgcgtcgac gcgcagccgg atgcgaccgt gttccatatc acgcaagacc gtaccgtgcg
     2701 cgtgtcgtgg aaggcggaga tccgcgccga actgcggcag atcttcaacg gcggcgaatt
     2761 caagccgatc ctcgaagaag cccaggcgat ccacaagaag gtgctgcgtg gccgcgtgtt
     2821 tgtcgcgctg cacatgcacg cgggcgacgg caacgtccac acgaacctgc cggtcaactc
     2881 cgacaactac gagatgctgc aggacgccca cacggcggtc gcgcgcatca tgaagctggc
     2941 gcgttcgctc gacggcgtga tttccggcga gcacggcatc ggcattacca agctcgagtt
     3001 cctgaccgat gaggaaatcg gcgaattccg cgcctacaag cagcgcgtcg atccgcatgg
     3061 ccgcttcaat gcgggcaagc tgctcgaagg cgccgacctg cgcaacgcct acacgccgag
     3121 cttcggcctg atgggctacg aatcgctgat catgcagcag tccgacatcg gcgcgatttc
     3181 ggaatcgatc aaggattgtt tgcgttgcgg caagtgcaag ccggtgtgcg cgacccacgt
     3241 gccgcgcgcc aacctgctgt acagcccgcg caacaagatt ctcgccacct cgttgctggt
     3301 cgaggcgttc atgtacgaag agcagacccg ccgcggtgtg tcgatcaagc actgggacga
     3361 gttcaacgac gtcgccgatc actgcactgt ctgtcacaaa tgcgtgacgc cgtgtccggt
     3421 gaagatcgac ttcggcgacg tgaccatgaa catgcgcaac ctgctgcgca agatgggcaa
     3481 gaagaagttc aacgccggtt ctgccgcggg gatgttcttc ttgaacgcga cgaatccgag
     3541 cacgatcaac gccgcgcgca cggcgatgat gggcatcggc tacaaggcgc agcgcctcgg
     3601 caacgacgtg ctgaagaagt tcgcgaagaa gcagacggcg cacccgccgg caaccgtcgg
     3661 caagccgccg gtcacgcagc aggtgatcca cttcatgaac aagaagatgc cgggcaacct
     3721 gccgaagaaa accgcgcgcg cg
//
LOCUS       DATASH010000705         3561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1499737,
            whole genome shotgun sequence.
ACCESSION   DATASH010000705 DATASH010000000
VERSION     DATASH010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1499737"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="VHV99_18715"
     CDS             <1..736
                     /locus_tag="VHV99_18715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018423505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX3637478.1"
                     /translation="PVDSEGLIVSKLPDKARLVYVTPSHQFPLGMPMSLERRVELLEW
                     AQKRGAVIIEDDYDCEYRFEGRPMEPLKSLDRAGLVAYLGTFSKTIFPELRIGYVVPP
                     ASLHGPLLKARQIADCHGCTLTQTALATFMLNGDFAKHLRRMHKTYAARRAMLLKHLQ
                     GDLAPWFEPIVPTAGIHLATRLKAPLTEEALVSAAREASIGLYGLAPFYRRAKPEPGV
                     MLGYGSIAVEHIDAALTKLAAILPRL"
     gene            complement(762..965)
                     /locus_tag="VHV99_18720"
     CDS             complement(762..965)
                     /locus_tag="VHV99_18720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637479.1"
                     /translation="MLQIVPGLFHMGPIRIIVTRHTGFAVAIGDGIALRDVGEIVTAA
                     KAALEWRRSPTTPEYPHIAADSR"
     gene            1432..1884
                     /locus_tag="VHV99_18725"
     CDS             1432..1884
                     /locus_tag="VHV99_18725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX3637480.1"
                     /translation="MPTSAKKVAKKAAAPVPTKKVAAKKVPAKKAVAAKKVAVKASSA
                     PSPIKDTFTKASLAAHVAERAAVEPKTAKAVLAALEDTILGAVHKKGAGEFTLSGLLK
                     IVVQAVPAKKKRFGKDPFSGEERWFPAKPASVRIKARPLKKLKDAAAG"
     gene            2420..>3561
                     /locus_tag="VHV99_18730"
     CDS             2420..>3561
                     /locus_tag="VHV99_18730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEX3637481.1"
                     /translation="MRCYDEMRHHDDAVRPHYARFERWLVKQGNEAIARKRAEADLLF
                     RRVGITFAVNGDLSGTERLIPFDLIPRIIPRSEWQTLEAGLRQRVQALNLFIHDVYHD
                     RNIVRAGIVPAEQVYTNAQYRPEMQGVNVPLGVYAHIAGVDVVRAGDEGEFYVLEDNL
                     RVPSGVSYMLENRKMMMRLFPELFVQNRIAPVAHYPDLLLDTLRSVAPEGVDDPVVVV
                     LTPGMYNSAYFEHTFLAQQMGVELVEGKDLFVDDNYVFMRTTQGPKRVDVIYRRVDDD
                     FLDPLAFRNDSALGVPGLLTAYRAGRVALANAMGTGIADDKSIYPYVPDMIEFYLGEK
                     PILNNVPTFQCRKPDDLAYTLAHLPELVVKEVHGAGGYGMLVGPAST"
BASE COUNT          679 a         1080 c         1098 g          704 t
ORIGIN      
        1 accggtggac agcgaaggcc tgatcgtgag caagctcccg gacaaggcgc ggctcgtcta
       61 cgtgacgccc tcgcatcagt tcccgctcgg catgcccatg agtctggagc ggcgcgtcga
      121 gttgctcgaa tgggcgcaga agcgcggcgc cgtgatcatc gaggacgatt acgactgcga
      181 gtaccgtttc gaaggccggc cgatggagcc gctgaaaagt ctcgaccgcg ccggtctcgt
      241 cgcttatcta ggcacgtttt cgaagacgat tttcccggag ctgcggatcg gctacgtggt
      301 gcctccggcc tccctccatg ggccgctcct gaaggcccgc cagatcgccg actgtcacgg
      361 ctgcacgctc acgcaaaccg cgctcgcgac gttcatgctc aacggcgact tcgccaagca
      421 tctgcggcgc atgcacaaga cttatgcggc gcgccgcgcg atgctgctga aacacttgca
      481 gggcgatctc gcgccgtggt tcgaaccgat cgtgccgact gccggcatcc atctggccac
      541 gcggctgaag gcgccgttga ccgaggaggc gctggtgagc gccgcgcgcg aggcgtcgat
      601 tggcttgtat ggactcgcgc cgttctatcg gcgcgcgaag ccggagcccg gcgtgatgct
      661 cggctatggc agcatcgccg tcgagcatat cgatgcggcg ctcacgaagc tcgccgctat
      721 tctgccgcgc ctctgaaact gccggctcgc tgcgaactac atcaacgcga atctgccgca
      781 atatgcgggt attccggtgt tgtcggtgag cggcgccatt caagggcggc tttggcggcg
      841 gtaacgattt caccgacgtc acggagggca atgccatcgc ctatcgcaac ggcgaacccg
      901 gtatggcgcg tgacgatgat gcgtattggg cccatgtgaa aaagaccggg cacgatctga
      961 agcacgtcag ccggccatga tcacgcgttg gaacgacgaa aaggacctgt gcttttctgt
     1021 cgcagaacaa cgttagccgt cacctgcaga gattttcgct gcagggggcg tgttttttcc
     1081 gccgcatgaa tcggcatggc gagcgcgtca gcgagccatg attcgactat cgagtcgccg
     1141 tccgttgctc atcgcctgac gccgaaagtg cctctattca tgcattgaaa cgacttgtaa
     1201 cgcgcgttac gtgttcgtgt ttccagacct ctttcaggca cgccgacaca cgtttacccg
     1261 ctaaaacaag catcaaaatg tgttgcgaag tctgcgcggc gcaacaaacc ttcgctgtta
     1321 tacacaaaag agtcgcgcaa atcgtattag cgcttgtaga atccggcgtt gaagcgtgca
     1381 cataccgtgt gcgcttcgtg caattcactg accactacag gtaggagaaa catgccgact
     1441 tccgcaaaaa aggtggccaa gaaggctgct gccccggtac cgaccaagaa ggttgctgca
     1501 aagaaagtcc ctgcgaagaa agccgtcgca gctaagaagg tcgccgtgaa ggcttccagc
     1561 gctccgtcgc cgatcaagga caccttcacg aaggcctcgc tggctgcaca cgtcgctgaa
     1621 cgcgctgctg tggaaccgaa gaccgccaag gccgtgctgg ccgcgctcga agacacgatc
     1681 ctcggcgctg tccacaagaa gggcgctggt gaattcacgc tgtcgggtct tctgaagatc
     1741 gtcgtgcaag ctgtgccggc gaagaagaag cgcttcggca aagacccgtt ttcgggtgaa
     1801 gagcgttggt tcccggccaa gccggctagc gtgcgcatca aggcacgtcc gctgaagaag
     1861 ctgaaagacg cagcagcagg ctgatcgcgc ttcatgcgtt agcgcggtat ttaaccgtgc
     1921 ggtttttggc cgcgtgtttt tagcagtgtg cttttgacgg ttcgctttta gccctccgct
     1981 tttagccctc cgcttttagc cgtccgcttt taaccgtcgt taacgtttga gccgacccgc
     2041 agtgctagtt gtccaaaaat ccccgtggat gcaaatccac ggggattttt tcatgcgcgg
     2101 tcccggcatt tttgcgaggt ggcgcgaacg cgccgcgatc ctggcgggag ttcggcttcg
     2161 ctccgatgga ggccctgtgt ccggttttac gatggccgct atcacccgcc acagtgcccc
     2221 tcatgcaccg tactagcgca tcatgggccg ccgcagtacg atggcagcga ggccgtgcca
     2281 gtcgggatgc gctcttggcg tgcatgcacc gtgtttcgca cgaaaagggc tcaatggcat
     2341 agcgcttgct tgatcgattg tcgaacaccg aatgcgcagc gcattcgcct tctatccgac
     2401 aacaagagcg gggcgtgaca tgcgatgcta tgacgagatg cgtcatcacg acgatgccgt
     2461 gcggccacac tacgcgcgct ttgagcggtg gctggtaaag cagggtaatg aggcgatcgc
     2521 acgcaagcgt gcggaagccg acctgctgtt ccgccgggtc ggcatcacct tcgcggtgaa
     2581 cggcgatctg tccggcaccg agcggctgat tcccttcgat ctgattccgc gcatcattcc
     2641 gcgcagcgag tggcagacgc tggaggccgg tttgcgccag cgggtgcagg cgctcaatct
     2701 gtttatccac gacgtctatc acgatcgcaa catcgtgcgc gccgggatcg tgccggccga
     2761 acaggtttat accaacgcgc aataccgtcc cgagatgcaa ggcgtgaacg taccgctcgg
     2821 tgtttacgcg catatcgccg gggtcgacgt ggtgcgcgcg ggcgacgagg gcgagttcta
     2881 cgtgctcgaa gacaacctgc gggtgccctc gggcgtgtcc tacatgctcg aaaaccgcaa
     2941 gatgatgatg cggcttttcc ccgagctgtt cgtgcagaac cgcattgcgc cggtcgcgca
     3001 ttatcccgat ctgctgctcg acacactgcg ctcggtggcg cccgaaggcg tggacgatcc
     3061 ggtcgtggtg gtgctcacgc cgggcatgta caactccgcg tatttcgaac acaccttcct
     3121 cgcgcagcag atgggcgtgg aactggtgga aggcaaggat ctgttcgtcg acgacaacta
     3181 cgtgttcatg cgcaccacgc aagggcccaa acgcgtggac gtgatctacc gccgggtcga
     3241 tgacgacttt ctcgatccgc tggcatttcg caacgactcg gcgctgggcg tgccggggct
     3301 gctcactgcc tatcgcgccg ggcgcgtcgc gctcgcgaac gcgatgggca ccggcatcgc
     3361 cgacgacaaa tcgatctacc cgtacgtccc ggacatgatc gagttttacc tcggcgaaaa
     3421 gccgattctc aacaacgtgc cgacgttcca gtgccgcaag cccgacgacc tcgcctacac
     3481 gctcgcgcat ctgccggagc tggtggtcaa ggaagtgcat ggcgcgggcg gctacgggat
     3541 gctggtggga ccggcgtcga c
//
LOCUS       DATASH010000706         3251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3080919,
            whole genome shotgun sequence.
ACCESSION   DATASH010000706 DATASH010000000
VERSION     DATASH010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3080919"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VHV99_18735"
     CDS             complement(<1..188)
                     /locus_tag="VHV99_18735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012217403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX3637482.1"
                     /translation="MHRFIVVGGGAGGLELATRLGDRYAPHKNKGGVHAQVTLVDRNP
                     THIWKPLLHEVAAGSMDP"
     gene            complement(366..3107)
                     /gene="polA"
                     /locus_tag="VHV99_18740"
     CDS             complement(366..3107)
                     /gene="polA"
                     /locus_tag="VHV99_18740"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006761695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEX3637483.1"
                     /translation="MPEERSLEGKTLLLVDGSSYLYRAYHAMPDLRGPEGGPTGALYG
                     MINMLRRMRKEVTAEYSACVFDAKGKTFRDDWYADYKANRPSMPDDLSRQIEPIHVAV
                     RALGWPLLMIEGVEADDVIGTLSTEAEKRGMNVIVSTGDKDLAQLVSDHVTLINTMTN
                     EKLDREGVIAKFGVPPERIIDYLSLIGDTVDNVPGVEKCGPKTAIKWLTQYETLDGIV
                     AHADEIKGAVGDNLRRALDFLPMAKKLVTVERACDLTGHIVSIEESLESRPESREELR
                     EVFTRNGFKTWLREVEIADAVEGPETDVPPALSVDHERHYDTVQTWEQFDAWLEKINA
                     AELTAFDSETTSLDPMTAQIVGLSFSVEPGHAAYVPLAHRGPDVPVQLPRDEVLAKLK
                     PWLESAEHKKVGQHMKYDEQVLANYGIEMRGVEHDTLLESYVVESHRTHDMDSLALRH
                     LGIKTIKYEEVAGKGASQIGFDEVALDKAAEYAAEDADITLRLHQALYPQVAAEKTLD
                     YVYRGIEVPTSRVLRKMERAGVLIDADKLRAQSSEIATRLIQLESEAYVLAGGEFNLG
                     SPKQIGQIFFEKLELPVVKKTPRGAPSTDEEVLQKLAEDFPLPKILLEHRGLSKLKST
                     YTDKLPRMVNAQTGRVHTNYAQAVAVTGRLASNDPNLQNIPVRTGEGRRIREAFIAPP
                     GHRLVSADYSQIELRIMAHISGDESLLRAFSQGEDIHRATAAEIFSVTPLEVSNDQRR
                     VAKVINFGLIYGMSAFGLAANLGITRDAAKLYIDRYFARYPGVARYMDETRLSAKAKG
                     YVETAFGRRLWLPEINGGNGPRRQAAERAAINAPMQGTAADLIKLSMIAVQKWIEESK
                     VGTRMIMQVHDELILEVPDAELSDVRKRLPELMCGVAALKVPLVAEVGAGLNWEEAH"
     gene            3109..>3251
                     /locus_tag="VHV99_18745"
     CDS             3109..>3251
                     /locus_tag="VHV99_18745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011881579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX3637484.1"
                     /translation="MTKRKVIPSLRSLADQERATAKKARASWQMFTIMAEFIEATEYL
                     SEIR"
BASE COUNT          580 a         1033 c          931 g          707 t
ORIGIN      
        1 aacgggtcca tgctgccggc tgccacttca tgcagcagcg gcttccagat atgggttgga
       61 ttacggtcga cgagggtcac ttgcgcgtgc acaccgcctt tgttcttgtg gggtgcatag
      121 cggtcgccta agcgcgttgc cagttccagc ccccccgcgc ccccaccaac gacaataaat
      181 cgatgcattg aactctccgt atttgccgat aggttatgcg tcgcgatggc gcgcattcaa
      241 caggaagcgc gctccgtctc ttctatcgat tgtccgcgag cggcacgggt tgtcacaagc
      301 catcaatacg tatatgtgac cagggtgcga cgttatggcc tggcacgcgc cccgtcgcat
      361 accagtcaat gcgcctcttc ccagttcagc ccggcgccca cttcagcgac cagcggcact
      421 ttcagcgcgg ccacaccgca catcagttcc ggcaagcgct tgcgtacgtc ggacaattcc
      481 gcgtccggca cttcgagaat cagttcatcg tgcacctgca tgatcatgcg cgtaccgacc
      541 ttcgactctt cgatccactt ctgcaccgcg atcatcgaca gcttgatcag gtcggcggcc
      601 gtgccctgca tcggcgcgtt aattgccgcg cgctccgctg cctgacggcg cggaccgttg
      661 ccgccgttga tttccggcag ccacaggcgg cggccgaacg ccgtttccac gtagcccttg
      721 gccttcgcgc tcagacgcgt ttcgtccata taacgcgcga cgcccggata acgcgcgaag
      781 tagcggtcga tatacagctt ggccgcatcg cgcgtaatgc cgaggttcgc ggcgagacca
      841 aacgcgctca tgccatagat caggccgaag ttgatgacct tcgccacgcg ccgctgatcg
      901 ttcgacactt cgagcggcgt cacgctgaaa atttccgccg cggtggcgcg gtgaatgtct
      961 tcgccttgcg agaacgcacg cagcaaggat tcgtcgccgg agatgtgcgc catgatacgc
     1021 aactcgatct gcgagtagtc ggcggagacg agcctgtgtc ccggcggggc gatgaacgcc
     1081 tcgcgaatcc ggcggccttc gccggtacgc acggggatgt tctgcaagtt cggatcgttc
     1141 gacgccaacc ggcccgttac cgccaccgcc tgtgcgtagt tcgtatgcac gcggcccgtt
     1201 tgagcgttga ccatgcgcgg cagcttgtcg gtgtaggtcg atttcagctt cgacaaaccg
     1261 cggtgttcga gcaggatctt cggcagcgga aagtcttcgg cgagcttttg cagcacttct
     1321 tcgtccgtgg acggcgcgcc tctcggggtc ttcttgacca ccggcaattc gagtttctca
     1381 aagaaaatct gaccgatctg cttcggcgaa ccgagattga attcgccgcc ggcgagcacg
     1441 tatgcttcgc tttccagttg gatcagacgc gtggcgattt cgctgctttg cgcgcgcagc
     1501 ttgtcggcgt cgatcagcac gcccgcgcgc tccatcttgc gcaacacgcg cgacgtgggc
     1561 acttcaatgc cgcgatacac gtagtcgagt gttttctccg cggccacttg cggatacaag
     1621 gcctgatgca gacgcaaggt gatatcggcg tcttccgctg catattcggc ggccttgtcc
     1681 agcgcgactt cgtcgaaacc gatctgcgag gcgcctttgc ccgccacctc ttcgtacttg
     1741 atcgtcttga tgccgagatg gcgcagcgcg aggctgtcca tgtcgtgcgt gcgatgcgat
     1801 tcgaccacgt acgattccag cagcgtatcg tgctcgacgc cgcgcatttc gatgccgtag
     1861 ttcgccaata cctgttcgtc gtacttcata tgctggccga ctttcttgtg ctcggcgctt
     1921 tccagccacg gcttgagctt cgcgagcact tcgtcgcgcg gcagttgcac cggcacgtcc
     1981 gggccgcgat gcgcgagcgg cacataagca gcatggcccg gctcgaccga gaacgacaat
     2041 ccgacgattt gcgcggtcat cggatcgagc gaggtagttt ccgaatcgaa cgcagtcaac
     2101 tccgctgcgt tgatcttctc gagccatgca tcgaactgct cccacgtctg cacggtgtcg
     2161 tagtgacgtt cgtggtccac gctgagcgcc ggcggcacat cggtctcggg cccttccacc
     2221 gcgtccgcga tctccacttc gcgcagccag gtcttgaaac cgttgcgcgt gaacacctcg
     2281 cgcagttctt cacgcgattc cggccggctc tccaggcttt cttcgatcga cacgatatgg
     2341 ccggtcagat cgcaagcccg ttcgacagtg acgagtttct tcgccatggg cagaaaatcg
     2401 agcgcacgtc gcagattgtc tcctaccgca cctttgatct cgtccgcatg tgcgacgatg
     2461 ccatcgagtg tttcgtactg cgtgagccat ttgatcgccg ttttcggccc gcatttctcg
     2521 acgcctggca cgttgtcgac ggtatcgccg atcaacgaca ggtagtcgat gatgcgctcc
     2581 ggcggcacgc cgaacttggc gatcacgcct tcgcggtcga gcttctcgtt cgtcatcgta
     2641 ttgatgaggg tgacatgatc cgacacaagc tgcgccagat ccttgtcccc agtcgacacg
     2701 atcacattca tgccgcgttt ttcggcttcg gtactgagcg tgccgatcac gtcgtcggct
     2761 tcgacgcctt cgatcatcaa cagcggccaa ccgagcgcgc gcacggcgac gtgaatcggc
     2821 tcgatctgac gcgacagatc gtccggcatc gacggacgat tcgccttata gtcggcatac
     2881 cagtcgtcgc gaaacgtttt gcccttggcg tcgaacacgc acgcgctata ctctgcagtg
     2941 acttccttgc gcatgcgccg cagcatgttg atcatcccgt agagcgcacc cgttgggcca
     3001 ccttcgggcc cgcgcaaatc gggcatcgca tggtaggccc ggtacaggta actcgaaccg
     3061 tcgaccaata gcagggtctt accttcaagg cttcgttctt caggcattat gactaagaga
     3121 aaagtgattc cgagtctgcg atcactcgca gatcaagagc gcgcaacggc taagaaggcc
     3181 cgcgcatcgt ggcagatgtt cacgattatg gcagagttta tcgaggcgac cgagtacctc
     3241 tcggagattc g
//
LOCUS       DATASH010000707         9724 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_510362,
            whole genome shotgun sequence.
ACCESSION   DATASH010000707 DATASH010000000
VERSION     DATASH010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 9724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9724
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510362"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(522..2888)
                     /locus_tag="VHV99_18750"
     CDS             complement(522..2888)
                     /locus_tag="VHV99_18750"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HEX3637485.1"
                     /translation="MTELEQGFILTRHWRDTPAGTEVDFWLATDGGPRHIRLRPQPSV
                     AFIPAGQRERAETVLRREAPLDLRPLDLCDFQHRPVMGLYCPQYRQLTGFEKRLKQGG
                     VDVYEADIFPPERYMMERFITAPVWFSGAMQQSGPLLNSELKPATNYRPPLKLVSLDI
                     ETSAHAELYSIALEGCGQRQVYMLGPPNGDAGDVDFDLEYCETRAQLLEKLNMWLARH
                     DPDAIIGWNLVQFDLRVLQQHAEQYRVPLRLGRGGAVMEWREHGRQQNHFFAGAAGRW
                     IIDGIEALRSATWSFPSFSLEHVSRSVLGEGKAIDNPYQRMDEIQRRFDEDKPALARY
                     NLKDCELVTRIFAKTELLPFLLERATVTGLPADRSGGSVAAFTHLYMPRMHRQGYVAP
                     NLGDVAGAASPGGFVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVDGLLNPGD
                     DQSVPGFLGARFSRTRHCLPSIVGQVWQGRETAKREQNKPLSQALKIIMNAFYGVLGS
                     TGCRFFDPRLASSITMRGHEIMHTTRELIQAQGYEVIYGDTDSTFVWLKHAHSEEEAS
                     RIGRALVEHINAQWRENLQTRFGLDSALELQFERHYKRFFMPTIRGAEEGSKKRYAGL
                     TILPDGSEDIVYKGLETVRTDWTPLAQQFQQELYRRIFKQQPYQDYVRDYVRDTLAGK
                     LDDQLVYRKRLRRPLGDYERNVPPHVRAARVADEFYRQQGRPLQYQNGGWISYVMTTA
                     GPEPLETLRSAIDYEHYLTRQLQPVADAILPLLRDDFTTLMSGQTQLF"
     gene            3156..3581
                     /locus_tag="VHV99_18755"
     CDS             3156..3581
                     /locus_tag="VHV99_18755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX3637486.1"
                     /translation="MNLSKLVVVVAVAASSFGAQAQVAGTQPLSVTVAQSNALLSGWS
                     VKKSILGKAVYNDQNEKIGSIRDLVVAPDGSISAAIVAAGGFLGVASHDVAVPVAALD
                     IRSGNFYLAGATKDALKATPEFQYNKVQSPPKPKKLTAQ"
     gene            complement(3872..4882)
                     /locus_tag="VHV99_18760"
     CDS             complement(3872..4882)
                     /locus_tag="VHV99_18760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 domain-containing protein"
                     /protein_id="HEX3637487.1"
                     /translation="MTIQLTPEFASKFANLALAHLTREYPNKLTHSLAGPQDVQGPRA
                     LHPIFYGSYDWHSCVHGYWLVLHLLERFPDLPEAARIVAVVDEHFTAANVAGELAYLK
                     LPHNGGFERPYGWAWLLALSAQLHSLKRSEAARWSKTLAPLTEFFVERFEEFLPKATY
                     PLRVGTHFNMAFALALTLDFARQTSREPLEALLVNTAERWFLNDVACQAWEPAGDEFL
                     SPSLMEAELMRRVLPPAQFVEWFRRFLPDIGAKKPATLFEPVTVTDRSDGKIAHLDGL
                     NLSRAWCQRSLARALPSGDVRRTVLFDSAERHLQSALPHVAGDYMGEHWLGTFATLAL
                     EA"
     gene            complement(4893..5843)
                     /locus_tag="VHV99_18765"
     CDS             complement(4893..5843)
                     /locus_tag="VHV99_18765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006399760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEX3637488.1"
                     /translation="MIKLESLYVLAGLMFAAFALFNLQDRSNPRRVVNFLFWGIYAVT
                     FMFGGELPHFVTGCLAIALALIAGSGKLGKGKTDANSEAAGARREAKAQRFGNKLFIP
                     ALLIPIITLLGTLTLKYVPFVDPKNVTLISLVLGTLVAFGTALVMLRDSPVHALKDAR
                     HTMDTVGWAAILPQMLAALGALFAIAGVGHVVSDLVKTWIPVDSAFAVVAAYTFGMAI
                     FTMIMGNAFAAFPVMTAGIGLPLIVHQFHGNPAILGAIGMLCGFCGTLMTPMAANFNI
                     VPAALLELKDQNGVIKAQWPTALLLLIVNTILLYLFVFRF"
     gene            complement(5840..6550)
                     /locus_tag="VHV99_18770"
     CDS             complement(5840..6550)
                     /locus_tag="VHV99_18770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEX3637489.1"
                     /translation="MWVLIGVPIVVLGFALRFNALLVVTIAGIATGVAGGLQPVEIVS
                     AFGKAFADNRYMGLIWLTLPVIALLERNGLKEQAKHLISRLHAATTGRVLMLYFVIRQ
                     ITAALGLTSLGGHAQMVRPLIAPMAEAAAANRYGELPDAVRQQIRANASAVDNIAVFF
                     GEDIFIAIQSILLIKGFLEQNGIMVEPLQVSVWAIPTAIAALVIHVVRLMLLDRNLSH
                     KMTTLGMERAQARGIAQS"
     gene            complement(6874..8076)
                     /locus_tag="VHV99_18775"
     CDS             complement(6874..8076)
                     /locus_tag="VHV99_18775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007583550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3637490.1"
                     /translation="MTHALLLSNVRLADGQAVSVAVADGRITALGPHVEPQAAALVED
                     GGGALLLSGFVEGHTHLDKSHWGRPWYRNEVGPTLVHRISNEREWRKTSGHDAAVQSL
                     ALARAFVQAGTTRLRTHVDVDTDARLRYVDGVLDTRQTMRDVLDMQIVAFPQSGMLIR
                     PGTVELLGNALAEGADVLGALDPALIDGDPVASLNATFELADRYRKPIDIHLHEPGEV
                     GAFTLKLLLDRVEAMGMQGRVVVSHAFCLGALPERERDVLLERIAHLRVALLPSAPPS
                     RPVPPLKVCRDKGVTIFGGNDGIRDTWSPYGVPDMLERAMLIGMRYDLRRDDDLEMAF
                     DCVSDAAAQGCGFSDYGLRVGARADLVLVDAESVAHAVVARPRRKLVVANGRIVVRDG
                     ALIDVKAA"
     gene            complement(8257..9579)
                     /locus_tag="VHV99_18780"
     CDS             complement(8257..9579)
                     /locus_tag="VHV99_18780"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX3637491.1"
                     /translation="MNAPTPGFNTLDRRADALIANSYYEASATRPLTDDPVLDGTLAA
                     DVCVIGAGFSGLSVALECRARGLSVVVLDAHRPGWGASGRNGGQTLVGFAKDEIIERQ
                     LGLDGARAAWAMSVEGVALVRERIERYGIDCDFTSGYLTVATKPKRVPDLRSWMESAS
                     QRWGYTKLSWLDTDAVRSRVASQRYLAGVYDPFSGHLHPLKYCLGLADAARREGAQLF
                     AQSPVIEVVRGARPVVRTAGGEVRCRFVAACGNATIGDVLPAPVAARIAPIASYIVAT
                     EPLGQARADALIKGREAICDNNFFLDYFRLSADHRVLFGGRASSTGASPAQLGEEIRK
                     RMIGVFPQLAEVRIDYAWGGFVDVTRNRAPDFGSIDPNYFYVQGFSGHGVALTGIAGR
                     VIAKAMAGETAAFDLFAQLRHARFPGGPALRGPALELGMMYHRILELF"
     gene            complement(9579..>9724)
                     /locus_tag="VHV99_18785"
     CDS             complement(9579..>9724)
                     /locus_tag="VHV99_18785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004525489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3637492.1"
                     /translation="IAEAIDGQASRFDLFGKIRHRDFPGGATLRTPALVLAMAWYRMK
                     DLL"
BASE COUNT         1956 a         2977 c         3023 g         1768 t
ORIGIN      
        1 ctcggggccg cactaggggc agcgtcgctc gcctgtgcat aggcatgaat tgaacccgag
       61 atcagaagga cggtgctgac cagtctgacg gcgtggattg ctttcatgca tcttctcctg
      121 tcgttgcaga tcgttggggt acagactctg ggatatcgca caacgccgcc acgatctttc
      181 cgtggcggat caaatggcac tatagaacaa taaagggtga ggtcaagcaa caatgcaaac
      241 cgcggccaat tgcgcacgca atcggcgatt ttagaatgcg tcagatagga cagcactcta
      301 attgtattga caggattgca aatcaagaat acgaatgagg ttttctctgc cttttatcac
      361 gggttatctc ttcctgttag acatgttccg ccatcccgga tgttgcagca cctgcgcggt
      421 gcacaaaatg ccgtctgatg agaaggctgc gccgccgtcc gtggtgaagt aggcaattcg
      481 tccatagggg cacgtaggcg atgcttgcgg cttcgtcaag atcagaacaa ctgcgtctgc
      541 cccgacatca gcgtcgtgaa atcgtcgcgc agcaaaggca gaatcgcgtc ggccaccggc
      601 tgcaattgcc gcgtcagata atgctcgtag tcgatcgccg agcgcagcgt ttcgagcggt
      661 tccggtccgg cggtcgtcat cacgtagctg atccagccgc cgttctgata ttgcaatggg
      721 cgcccctgtt gccggtagaa ctcgtcggcc acgcgtgccg cgcgcacatg cggcggcacg
      781 ttgcgctcgt aatcgccgag cggcctgcgc agccgcttgc ggtacaccag ctgatcgtcc
      841 agttttcctg ccagcgtatc gcgcacatag tcgcgcacat aatcctgata gggctgttgc
      901 ttgaagattc gccgatacag ttcctgctga aactgctggg cgagcggcgt ccagtcggta
      961 cgcactgttt cgagcccttt gtagacgatg tcctcgctgc cgtccggcaa aatagtcagc
     1021 ccggcatagc gtttcttgct gccttcctcg gcaccgcgaa tcgtcggcat gaaaaagcgc
     1081 ttgtagtgcc gctcgaattg caattcgagc gcgctgtcga gtccgaaacg cgtttgcaga
     1141 ttctcccgcc actgcgcgtt gatatgctcg acaagcgcgc ggccgatgcg gctcgcttct
     1201 tcctcgctgt gcgcgtgctt cagccacacg aaagtcgaat ccgtatcgcc gtaaatgacc
     1261 tcgtagcctt gcgcctggat cagctcgcgc gtggtgtgca tgatttcatg gccgcgcatg
     1321 gtgatcgacg acgcgagacg cggatcgaag aagcggcagc ctgtggatcc gagcacgccg
     1381 taaaacgcgt tcatgatgat cttcagtgcc tgcgaaagcg gtttgttctg ctcgcgtttg
     1441 gcggtttcgc gcccctgcca cacctggccg acgatcgacg gcaaacaatg gcgtgtgcgc
     1501 gaaaagcgtg cgccgagaaa gcccggcacc gattggtcgt cgccgggatt cagcaggccg
     1561 tccacgagac ccaccgggtc gatcagaaac gtgcggatga tcgacgggta cagactcttg
     1621 tagtcgagca cgagtacgga atcgtagaga ccgggccgtg aatccatcac gaagccaccg
     1681 gggctcgcgg caccggctac atcgccgaga ttcggcgcga cgtagccttg ccgatgcatc
     1741 cgcggcatat acagatgcgt gaacgccgcg accgatccac cgctgcgatc cgcgggcaaa
     1801 ccggtgaccg tcgcgcgttc cagcagaaac ggcagcaact cggtcttcgc aaagatacgg
     1861 gtgaccagct cgcaatcttt caggttgtag cgcgcgagcg cgggcttatc ctcgtcgaaa
     1921 cggcgctgga tttcgtccat gcgctggtac ggattgtcga tcgccttgcc ttcgcccaat
     1981 accgagcgcg agacgtgttc gaggctgaac gaagggaaac tccacgttgc cgagcgcaac
     2041 gcttcaatgc cgtcgatgat ccatcttccc gcggcgcctg caaagaaatg gttctgctgg
     2101 cggccgtgct cacgccattc catcaccgca ccgccgcggc cgagccgcaa cggcacgcga
     2161 tattgttcgg cgtgctgttg cagcacacgc agatcgaact gcacgaggtt ccagccgatg
     2221 atcgcgtcgg ggtcgtgccg tgccagccac atgttcaact tttcgagcaa ctgtgcacgc
     2281 gtttcgcagt attcgaggtc gaagtcgaca tcgccggcgt cgccgttcgg cggtcccagc
     2341 atatagacct gacgttgtcc gcaaccttcg agcgcgatcg aatagagctc ggcgtgcgcg
     2401 ctggtttcga tatcgagcga gaccagcttc aatggcggcc ggtagttcgt ggccggtttc
     2461 aattcgctgt tcagcagtgg gccgctttgt tgcatggcgc cgctgaacca caccggcgcg
     2521 gtaatgaacc gctccatcat gtagcgctcg ggcgggaaga tgtcggcctc gtagacgtcg
     2581 acgccgcctt gtttcaggcg tttctcgaag ccagtcaatt gccggtactg ggggcagtaa
     2641 agccccatca ccggccgatg ctggaagtcg cacagatcga gcgggcgcag atcgagcggt
     2701 gcttcacggc gcaggaccgt ttccgcgcgc tcgcgctgcc cggcgggaat gaaggccacc
     2761 gaaggctgag gacgcaggcg gatgtgccgg ggcccgccgt ctgttgccag ccagaaatcg
     2821 acttccgtgc cggcgggcgt atcccgccaa tgtcgggtca aaatgaaacc ctgttcaagc
     2881 tcagtcaaac tgccacctct aaacgggcgt catcacctga cgcgatttta ccgttatgtg
     2941 gttgttgcag cggctgtata ggcgcgatcg caaccgggtt ggcgggtcac tgatgcaacc
     3001 ctggctccgc gggacggtag gactgcgcag agtccggcat ttgcagaaag cagtctgaat
     3061 gcagacgcgc cgcgtagtct gtatgatcgt tttgcagcga ttccttcggc gcagtgcacg
     3121 tgccggtcaa gtctaccagg aacggagtga gcgatatgaa cttgagcaag cttgttgtcg
     3181 tggtcgcggt agctgcaagc agttttggtg cacaggcgca agtcgccggc acgcaaccct
     3241 tgagcgtcac ggtcgcgcag tcgaacgcgc tgctgagcgg ctggagtgtg aagaagagca
     3301 ttctcggcaa ggccgtgtac aacgaccaga acgaaaagat cggttcgatc cgcgatctgg
     3361 tggtggcgcc cgacggttcg atttccgctg caattgtcgc ggcgggcgga ttcctcggcg
     3421 tggcgtcgca tgacgtagcg gttcctgtcg ctgcgctcga tattcgttca ggcaatttct
     3481 atctggcagg cgcaaccaaa gatgcgttga aggcgacgcc cgagttccag tacaacaagg
     3541 ttcaatcgcc gccgaagcct aaaaagctga ccgctcagta atgcctggaa ctgtgcccgg
     3601 aaccgtgcct ggaaccgtgc ctggaaccgc tcgctttgcg gatcggcttt tttgctcatc
     3661 aaccgcagcg cgtctacctc gcgaatcggg tgcggaaacg atggacctcg cacggccgca
     3721 ttctcaagcg acaccgctgt aacggttagc gcctgaatca cgcggcaaaa taaaacggcg
     3781 cttgcggcgc gaatcggtag tgtgccaaaa ccgtttcacg ctcgcagcgc cgtttttgct
     3841 ttcagtgagg agggcactgt cagcccatcg tttacgcttc caaagctagc gtggcgaacg
     3901 tgcccaacca gtgttcgccc atatagtcgc cggccacgtg cggcagggcg ctttgaagat
     3961 ggcgctcggc cgaatcgaac aataccgtcc ggcgcacgtc accggagggc aacgcgcggg
     4021 ccagcgaccg ctggcaccat gcgcggctca gattcaggcc atccaggtgg gcgatcttgc
     4081 catcgctgcg gtcggtcacc gtaacaggct cgaacagcgt ggcgggcttt ttcgcgccaa
     4141 tgtccggcag aaagcgccgg aaccattcga cgaattgcgc cggcggcaaa acgcgccgca
     4201 tcaactcggc ttccatcagc gaaggcgaca aaaactcgtc gccggcgggc tcccaggcct
     4261 gacaggcgac atcgttcaga aaccagcgct ccgccgtatt caccagcagc gcctcaagcg
     4321 gttcacgcga agtttgccgc gcgaaatcga gcgtgagcgc cagcgcgaat gccatgttga
     4381 agtgcgtgcc gacgcgcaac gggtaggtcg ccttcggcag gaattcctcg aaccgctcga
     4441 caaagaactc ggtaagcggc gccaacgttt tggaccagcg cgcggcttcc gagcgtttca
     4501 gcgaatggag ttgcgcgctc agcgcgagca gccacgccca cccatacgga cgctcgaagc
     4561 cgccgttatg cggcagcttg agataggcaa gctcgccagc cacattcgcg gcggtgaagt
     4621 gctcgtcgac caccgcgacg atgcgcgccg cttcaggcag atccggaaag cgctcgagca
     4681 gatgcaggac cagccagtag ccgtgcacgc acgaatgcca gtcgtagctg ccgtaaaaaa
     4741 tcggatgcaa tgcgcgcggg ccctgcacgt cttgcggacc ggcaagcgag tgcgtcagct
     4801 tgttcggata ctcgcgcgtg aggtgggcga gtgcgagatt ggcgaatttg gacgcgaatt
     4861 cgggggtgag ttgaatagtc atcggcgcct cctcaaaagc ggaatacgaa cagatacaac
     4921 agtatggtat tgacgatcag caatagtaaa gcggtcggcc attgcgcctt gatcacgccg
     4981 ttctggtctt tcagttccag cagcgcagcc ggcacgatat tgaagttggc cgccattggc
     5041 gtcatcaacg tgccgcagaa gccgcacagc atgccgatgg cgccgagaat cgccgggttg
     5101 ccatggaatt gatgcacgat caacggcaag ccgatgcctg ccgtcatgac cggaaaggcg
     5161 gcgaacgcat tacccatgat catggtgaag atcgccatgc cgaatgtata ggcggcgacc
     5221 acggcgaaag cggagtcgac tggaatccag gtcttgacga gatccgacac cacgtggccc
     5281 acgccggcga tcgcaaacag cgcgccgagg gcagccagca tctgcggcag gatggcggcc
     5341 caacccacgg tatccatggt gtgccgcgcg tctttcaacg cgtgcaccgg cgagtcgcgc
     5401 aacatgacaa gcgccgtgcc aaaagcgaca agcgtgccta gcaccagcga aatcagcgtg
     5461 acgttcttcg ggtcgacgaa cgggacatat ttcagcgtca aggtgccgag caaggtgatg
     5521 atcggaatca gcagcgccgg gatgaaaagc ttattgccga agcgctgtgc tttggcttcg
     5581 cggcgcgcgc cggccgcttc gctgttggcg tccgttttgc ctttgcccag cttgccggaa
     5641 ccggcgatca gggcgagcgc gatggcgagg cagccggtca cgaaatgcgg cagttctccg
     5701 ccgaacatga aggtgaccgc gtaaatgccc cagaacagaa agttgacgac gcggcgcgga
     5761 ttcgagcggt cctgcagatt gaagagcgcg aacgcggcaa acatcaaacc ggccagcacg
     5821 tagagggatt cgagcttgat catgattgtg cgatacctcg tgcctgtgcc cgttccatgc
     5881 ccaacgtagt catcttgtgc gacagattgc ggtcgagcag catcaggcgc accacgtgaa
     5941 tcacgagtgc ggcgatcgca gtgggaatgg cccacaccga cacctgcagc ggttcgacca
     6001 tgatgccgtt ctgttcgagg aagcccttga tcagcaggat cgactggatc gcaatgaaaa
     6061 tgtcttcgcc gaagaacacg gcaatgttgt cgaccgccga cgcattcgcg cgaatctgct
     6121 gccgcacggc atcgggcagt tcaccgtagc gattcgcggc ggctgcctcg gccatcggcg
     6181 cgatcagcgg ccgcaccatc tgcgcatgtc cgccgagcga cgtgagtccg agcgcggcgg
     6241 tgatctggcg aatcacgaaa tacagcatca gcacgcgccc ggtggtggcg gcgtgcaaac
     6301 gtgaaatcag gtgtttggcc tgttctttca ggccgttgcg ttcgagcagc gcgatcaccg
     6361 gcagcgtgag ccagatcagg cccatgtagc ggttatccgc gaacgctttg ccgaatgcgc
     6421 tgacgatctc cacgggttgc aagccaccgg ccacgccggt tgcgatcccc gcgatcgtca
     6481 ccaccagtag cgcgttgaag cgcaatgcaa agccgagtac gacgatcggc acgccaatca
     6541 aaacccacat cgtgattctc ctgtgtcgct gctatgtcgt tgcttatgtc gttgttttgc
     6601 cgctgcgcgc gccgctttgg gcgcttggcg agagccggtt gcatttgggt gtctggcggg
     6661 ttgcgccagg ttgcaacggc tcgcgggcga cgaaatctaa cacgataatt tatcgataaa
     6721 tagtcaacaa aatgcgttta ccgggtaaac gccaggggcg acgagtggcc gcggcgcgcg
     6781 gcaaggccgc cgatgcgtgg cgcgcttcct cgagagaagc acgtcaatca ctgctcgtgg
     6841 atgaagactc ttccagtgag gtacgcgcgc gtcttatgcc gccttgacgt cgatcagcgc
     6901 gccgtcgcgc acgacgatac gtccgttcgc cacaaccagc ttgcgcctgg gccgggcaac
     6961 cacggcatgc gcgacggatt ccgcatcgac cagcacgagg tccgcgcgtg cgccgacgcg
     7021 cagaccgtag tcgctgaaac cacaaccttg agcggcagca tcgctcacgc agtcgaaggc
     7081 catctcgagg tcgtcgtcgc gccgcaagtc gtagcgcatg ccgatcagca tcgcgcgctc
     7141 gagcatgtcc ggcacgccat acggcgacca ggtatcgcga atgccatcgt tgccgccgaa
     7201 gatcgtgacg cctttgtccc tgcaaacttt gagcggcgga accgggcgag acggcggcgc
     7261 gctgggcaga agcgccacgc gtagatgcgc gatccgttcg agcagcacgt cgcgttcacg
     7321 ttccggcaag gcgccgaggc agaacgcgtg actgaccaca acccggccct gcatgcccat
     7381 tgcctcgacg cgatcgagca gcagtttcaa tgtgaatgcg ccgacttcgc cgggttcgtg
     7441 cagatggata tcgatcggct tgcggtagcg gtccgcgagc tcaaaggtcg cgttcagcga
     7501 cgcaacggga tcgccgtcga tcaaggcggg atcgagcgcg ccgagtacgt cggcgccttc
     7561 ggcgagcgcg ttgccgagca gctcgaccgt gccggggcgg atcaacatgc ccgattgcgg
     7621 aaacgcgacg atctgcatat ccagcacgtc acgcattgtc tgccgcgtgt cgagcacacc
     7681 gtcgacataa cgcagccgtg catcggtatc gacgtcgaca tgcgtacgca ggcgcgtcgt
     7741 gccggcctgc acgaatgccc gcgccagcgc gagcgactgg acagccgcat cgtgtcccga
     7801 cgtcttgcgc cattcgcgct cgttgctgat gcggtggact agcgtgggtc cgacttcgtt
     7861 gcgataccac ggacggcccc agtggctttt gtcgaggtgc gtgtgccctt cgacgaaacc
     7921 cgacagcagc aacgcgccgc cgccgtcttc gactaaagca gcggcttgcg gttcgacatg
     7981 cggcccgagc gccgtgatgc gaccgtcggc gaccgctacg gaaaccgctt ggccgtcagc
     8041 cagccgcacg ttggagagga gaagagcgtg agtcatgttg gtcataccga ttccacggag
     8101 ggggcgatac gggcccgcaa ggcgcgctgc tttatcagcc gacggccgat gccgtcgtca
     8161 aggaacttgc gcgtgcgttg tcacgtatgt tcgggccgca gcggcggtga agcgcttgcg
     8221 cattggcgtg catcaacaat aacgccggac aggcgcctaa aacaactcca gaatccggtg
     8281 atacatcatc ccgagttcaa gcgccggacc acgcaacgcc ggcccgccag gaaagcgtgc
     8341 atgccgcaac tgcgcgaata ggtcgaaagc cgccgtttca cccgccatgg ccttcgcaat
     8401 cacgcgtccg gcaattccgg tcaacgccac accatgcccg gaaaagccct gcacatagaa
     8461 gtagttcgga tcgatcgagc caaaatcggg cgcgcgattg cgcgtgacgt cgacgaaacc
     8521 gccccacgca taatcgattc gcacctcggc gagttgcgga aacacgccga tcatgcgctt
     8581 gcgaatctct tcgccgagtt gcgcaggcga ggcgccggtc gaacttgcgc gtccgccgaa
     8641 cagcacgcga tgatcagccg acagacggaa ataatcgagg aagaagttat tgtcgcaaat
     8701 cgcttcgcgc cctttgatca acgcatcggc acgtgcctgg ccgagcggct cggtggcgac
     8761 gatgtaagac gcgatcggcg cgatgcgcgc ggcgaccggc gcgggcaaca cgtcgccgat
     8821 cgtcgcattg ccgcacgccg cgacgaagcg gcagcgtact tcgccgccag cggtacgcac
     8881 aacaggccgc gcaccgcgca ccacttcgat caccggcgat tgcgcgaaca gttgcgcacc
     8941 ttcacgacgt gccgcatcgg cgaggccgag gcagtatttg agcggatgca ggtggcccga
     9001 aaacggatcg tagacgccgg ccagatagcg ttgcgaagcg acgcgtgaac gcactgcatc
     9061 ggtatcgagc cacgagagct tcgtataacc ccagcgttgc gaggccgatt ccatccacga
     9121 acgcaaatcc ggcacacgtt tcggtttggt cgccaccgtc agataaccgg aggtgaaatc
     9181 gcaatcgatc ccgtagcgtt cgatgcgctc gcgcacgagc gccacgcctt ccaccgacat
     9241 cgcccatgcc gcgcgggcgc cgtcgagtcc gagttggcgc tcgatgatct cgtccttggc
     9301 gaagccgacc agcgtctgac cgccgttacg gcccgacgcg ccccaaccag ggcggtgcgc
     9361 atcgagcacc accaccgaca agccgcgcgc ccggcattcc agcgccaccg acaaaccgga
     9421 gaaccccgcg ccgatcacgc agacgtcggc tgccagcgta ccgtcgagca cgggatcgtc
     9481 ggtcagcgga cgggtggcgc tcgcctcgta ataggagttg gcgatcagcg cgtcggcgcg
     9541 ccggtcgagg gtattgaagc caggcgtcgg agcattcatc agagcaggtc cttcattcga
     9601 taccaggcca tcgcgaggac gagggcaggg gtgcgcagtg tagcgccgcc cgggaagtcg
     9661 cggtgacgaa tcttgccgaa cagatcgaaa cgcgacgcct ggccgtcgat cgcttcggcg
     9721 atca
//
LOCUS       DATASH010000708         2210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6839550,
            whole genome shotgun sequence.
ACCESSION   DATASH010000708 DATASH010000000
VERSION     DATASH010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6839550"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1725)
                     /locus_tag="VHV99_18790"
     CDS             complement(<1..1725)
                     /locus_tag="VHV99_18790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX3637493.1"
                     /translation="MNDPRIPPDNLNRRRFLAAGSVMVSFTLLPAVRALAQTTTTEAG
                     TFTASAPQLPGSLKISPMLDAWVKIDEAGRITVFTGKAELGTGIRTAFMQIAAEQLDV
                     TPERIVLVTADTNATPDEGYTAGSHSTADSGTAIANATAQVRSLLLVAAGTRLNVDVA
                     SLTTQDGEIVATDGRRLTFGEAVSGVDLHRNAVPGAPTKDPKSFKVIGQSFHRVDIPG
                     KVTGAPRYVQDLKLPGMVHARVVRPPSYGATLQSVDIKPVRAMPGVIAVVQDGSYLAV
                     VANDEWCAIVAMRALSDAAKWHSGPPLPAQSTIHETLEKLPAQEISVANKTAAAAPAT
                     QTLHARFTKPYLTHGSIGPSCAVAWLNDGTMTVWTHTQGVFPMRKGLAEMLSMPPERV
                     RCIHVEGSGCYGHNGADDVAADAALIARAVPGKPVRVQWMREQEHTWEPFGPAMSSDV
                     TASLDASGQIVDWKFEVWSNTHNNRIENAGRFMPTWFLSKPFTPAPPKPIPMPEGGGD
                     RNSIPLYRLPNLYVQHHFLPDMPIRVSAMRSLGAYHNIFSIESFIDELALAAHTDPVA
                     FRLKHLDDP"
     gene            complement(1722..2177)
                     /locus_tag="VHV99_18795"
     CDS             complement(1722..2177)
                     /locus_tag="VHV99_18795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006498476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX3637494.1"
                     /translation="MMTLIVNGMPHQLDIDPSTPLLYALRNQLGLPGAKFGCGLGQCG
                     ACTVIVGDKPVFSCLMPVAAIGNRSVRTIEGLGSTDKPGKLQQAFIDKQAAQCGYCIA
                     GMVMRAQALLDRVPSPTDEQIRAHMEPNLCRCGTHMRILAAIRQAGQPA"
BASE COUNT          380 a          686 c          729 g          415 t
ORIGIN      
        1 cggatcgtcg agatgcttca ggcggaacgc aaccggatcg gtatgcgcgg cgagcgcgag
       61 ttcgtcgatg aaactctcga tcgaaaagat gttgtgatac gcgccgagcg aacgcatcgc
      121 cgacacgcgg atcggcatgt ccggcaggaa atgatgctgc acgtagagat tcggcaagcg
      181 atacagcgga atgctgttgc ggtccccacc gccttcgggc atcggaatcg gcttcggcgg
      241 cgcgggcgta aagggcttcg acagaaacca cgtcggcatg aaacgcccgg cgttttcgat
      301 gcgattgttg tgcgtgttac tccacacctc gaacttccag tcgacgatct gtccgctcgc
      361 atccaatgat gccgtcacgt cgctcgacat cgccggtccg aacggctccc acgtgtgctc
      421 ctgctcgcgc atccattgca ctcgcacggg ctttccaggc acggcgcgcg cgatcaacgc
      481 ggcgtcggcg gcgacgtcgt cggcaccgtt gtgtccgtag caaccggagc cttcgacgtg
      541 gatgcaacgc acgcgctcgg gtggcatcga cagcatttcg gcgagccctt tgcgcatcgg
      601 aaatacgcct tgcgtgtgcg tccagaccgt catcgtgccg tcgttgagcc acgcgacggc
      661 acacgacggg ccgatcgatc cgtgcgtcag atacggtttc gtgaagcgcg catgcaaggt
      721 ctgcgtggcg ggcgcggcgg cggccgtttt gtttgcgacg gagatttcct gcgcgggcag
      781 tttttcgagc gtctcgtgga tcgtcgactg cgcgggcaac ggcggaccac tgtgccattt
      841 cgcggcgtcg gacagcgcgc gcatcgcgac gatcgcgcac cattcatcgt tggcgacgac
      901 cgcaagatag ctgccgtcct gcacgacggc gatcacgccg ggcatcgcgc gtaccggctt
      961 gatatcgacc gattgcagcg ttgcgccgta agacggcggc cggacgacgc gcgcatgcac
     1021 catgccgggc aacttcaagt cctgcacata acgcggcgcg ccggtgacct tgcccggaat
     1081 atccacacga tgaaacgact ggccgatcac cttgaacgac ttcggatctt tggtcggcgc
     1141 accgggcacg gcattgcgat gcaagtcgac gccactgacc gcttcgccga atgtcaatcg
     1201 acggccgtcg gtcgcgacaa tttcgccgtc ctgcgtcgtc agcgatgcga cgtcgacgtt
     1261 cagtcgcgtg cccgcggcaa cgagcaacag cgagcgcacc tgcgcggtcg cgttggcgat
     1321 tgcggtgccg ctgtcggcgg tcgaatgact gcctgcggtg tagccttcgt cgggcgtcgc
     1381 gttcgtgtcg gcggtgacga ggacgatgcg ctcgggcgtc acgtcgagct gttcggcggc
     1441 gatctgcatg aacgccgtgc gaatgccggt gccgagttcc gccttgccgg tgaacaccgt
     1501 gatgcggccc gcttcgtcga tcttcaccca cgcgtcgagc atcggcgata tcttcagact
     1561 gcccgggagc tgcggtgcgg acgcagtgaa cgtgccggct tcggttgtcg tggtctgcgc
     1621 gagcgcgcgt acggcgggca acagcgtgaa gctgaccatc acgctgccag ctgcaagaaa
     1681 gcggcggcga ttgaggttgt caggcggaat gcgcggatcg ttcatgcggg ttgccccgcc
     1741 tgacgaatcg cggcaagtat gcgcatgtgc gtgccgcagc ggcacagatt cggctccatg
     1801 tgcgcgcgaa tttgctcgtc ggtgggcgac ggtacacgat cgagcagcgc ctgcgcgcgc
     1861 atcaccatgc ccgcgatgca gtagccgcac tgcgccgcct gtttatcgat gaatgcctgt
     1921 tgcagcttgc cgggcttgtc cgtgctgccg agtccttcga tggtacgcac ggaacggttg
     1981 ccgatggccg cgaccggcat caaacacgag aacaccggct tgtcaccgac gatcaccgta
     2041 cacgcgccgc attggcccag accacagccg aatttcgcgc cgggcaggcc gagttgattg
     2101 cgcagagcgt agaggagtgg cgtcgacgga tcgatatcga gttgatgcgg catgccgttc
     2161 acaatgagcg tcatcatggt tcgtgagact ccttgcgcag gcgttcgacg
//
LOCUS       DATASH010000709        11694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5061774,
            whole genome shotgun sequence.
ACCESSION   DATASH010000709 DATASH010000000
VERSION     DATASH010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 11694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11694
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5061774"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            6..926
                     /locus_tag="VHV99_18800"
     CDS             6..926
                     /locus_tag="VHV99_18800"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX3637495.1"
                     /translation="MATPTYRGVFPVAPTVFDTAGRLDLDGQRRAIDFMIDAGSHGLC
                     ILANFSEQFALTDDERALVQKTVLEHVAGRVPVIVTTTHFSSDVCAARSRAAQDAGAA
                     MVMVMPPYHGATIRVGERGIYEFFDRVSDAVGIPIMIQDAPVAGTPLSASFLAKLAKE
                     IANVSYFKIETAQAASKLRELIELGGDAVIGPWDGEEAITLIPDFDAGATGAMTGGGY
                     PDGIRKIVDAYTSGDTEGAAALYEKWLPLINYENRQCGLAACKALMMEGGVIRSDTTR
                     HPFAAMHPSTRAGLMKVARRLDPLVLSWGK"
     gene            983..2566
                     /locus_tag="VHV99_18805"
     CDS             983..2566
                     /locus_tag="VHV99_18805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016355105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase (NADP(+))"
                     /protein_id="HEX3637496.1"
                     /translation="MEISSDLLIGKQSVHGTNGSIKGINAATGEPLAPAFGGASLDQL
                     EQACALAWQASDIYRELPLETRATFLETIAQNILDIGDALIDRCVAESGLPRGRIEGE
                     RGRTVGQLRMFAAVVREGDFLGVRIDPAQPQRQPLPRVDLRLRYVPLGPVAVFGASNF
                     PLAFSVAGGDTASALAAGCPVIAKAHSAHPGTSALVGRAVQKAVADCGLPEGTFSLLF
                     DSGRDIGQGLVSDRRIKAVGFTGSRGGGTALMKLAAARREPIPVYAEMSSINPVLLFP
                     HALKNRGAAIGKAFAGSLALGAGQFCTNPGLVLAIDGPELDAFIEETGAALSETPAAT
                     MLTPGIHKAYESAVNKTAEHGEVKTLARGCAAKGATQGQAALFSTTAAAFRANPELHE
                     EIFGAASLIVRCPDLATVLELVEQLEGQLTAALHIDEADYDDARRFLPALERRTGRIL
                     VNGFGTGVEVGHAMVHGGPFPSTADGRSTSVGSLAIDRFLRPVSYQDMPEGLLPDALK
                     IANPLGLNRRLDGKLQLSK"
     gene            complement(2597..3337)
                     /locus_tag="VHV99_18810"
     CDS             complement(2597..3337)
                     /locus_tag="VHV99_18810"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX3637497.1"
                     /translation="MGGGFTREGDLKNTASYPLWLQEVLLETGAAKNRVTSHPLFRSL
                     RDGELGPLEMRAFLINGWPVINQFPQYMGMNLQKIGPEETPGSRMARRYLTRNIRVEQ
                     NHADYWVDWAAAHDVTREDLQAGEAPALAYALSHWCWKSSNADPLHASIAATNFAVEG
                     VTGEWSTLLCSRDTLAHSYPEPVRKKTMRWLRMHTHYDDAHPWEALDIVATMLGRTPR
                     MADVREVKRSIQRSYEYFEVSLTCCLEA"
     gene            complement(3337..5796)
                     /locus_tag="VHV99_18815"
     CDS             complement(3337..5796)
                     /locus_tag="VHV99_18815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011401601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX3637498.1"
                     /translation="MDMPSKGENAQLIRITWPFLVVILVLVSLAIFSIDIMSSVRAYI
                     GGESIWSKGQKDAAFYLNLYAETGSESTYKQYLTAMEGPRNLRTARLALDQSKPDPVT
                     ARRALIASGVHPDDVSDVIWTFKMFRHVSYFSEAVGYWSRGDAEMAKLERVGNELHAR
                     VAAGELPVALVSAYKSQIWTINATIAPLSWGFSQVLGTAFRKTASLLLTVDLVAALLL
                     VCLSTVHVRRLIRQRGRIEAALKQSEARAKATLGSIGEAVIATGPSGHIEFINPAAER
                     IAGRLADDCLGRPLVQTFRLLHADSRQPLNPIQDARAGARPDGESAEMLLQRRDGAEI
                     RVQAVTSRISAAGASPNMANAESGFVLILRNMTREYEYIESLAWQATHDGLTALVNRA
                     EFERRLQHALGTRWSEVASWLPAASALLFLDLDRFKVVNDTCGHAAGDAMLREVALRF
                     EASLQPDDTLARLGGDEFGVLLQGYDTPGIQEVAERLRACLNDFVFNWEAQPFTTSVS
                     VGVVDLRNTEMSVEEAMRLADIACYMAKERGRDRVQLADLGDRELARHASEVSWGRRL
                     KQALEHDDFCLYVQPIVAVGDGLPASAEGQRAELLLRMLDPGVINGVIAPTLFIPAAE
                     RYGLMTAIDRWVIRNAFDMLSRTRHKRFKEYAINLSGASVGDERFLGFVREQFQRTGV
                     SPGMICFEITETTAIANLASAARFMHELNALGCHFALDDFGAGMSSFGYLKHLPVEYL
                     KIDGSFVTDMVLDPVSREMVAAINEMGHSMKCRTIAEYVESEGILRALHALGVDYAQG
                     YYIGRPTLWTEPAVLTLAKEA"
     gene            6358..8097
                     /locus_tag="VHV99_18820"
     CDS             6358..8097
                     /locus_tag="VHV99_18820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637499.1"
                     /translation="MARLFFPTSAGSVTCKRCGNDIPSDADTCPHCGADHGPGFGAAK
                     TAALATGLRLPFGARREALNIPLPYPSVPEESELAGTGEQRWDMSKTITLGAVALALV
                     AGGVIYSQHGSSSGDDTTRSELPAGHSAYGAIDMKTAHDAPARPAAPAVPPARSAAAA
                     TATTARPAQTAAAPSVAAREALPTDTSSARAATALASTTDNLQAARDAITRGDLTTAR
                     RRFSKIPASQLSAANVQRTLADLTGLEHARDEMLQVARGCEATGSWLCVRQNARDVLT
                     IDASNAEAQALVEHAIARSGWLNKPTSAPATTAAHGARHNNAQTAAAAPAPAVPAPMY
                     VPGPRVAATPPPASTHKPAVTVSAAEHARAVAAMGAPTPIPTPVAAAPVAPTVVATMR
                     SPVTAQAVPGTGSQAPLTSSPTTSPQPRQQAAPARPPVPSAPAFVPMPDGPDAMPATA
                     PVRSSPAAATARAPAQPEPEPATAAQVPGDTASPPVVRQLAPISEAVPVRAGIVSPPV
                     AANGAPAGVQNITRSPSPAVHRTLASGDNATSAAQPHAPAFNTTNSDDEERAILESGW
                     SKSKSSAQRSTPQ"
     gene            8263..9348
                     /locus_tag="VHV99_18825"
     CDS             8263..9348
                     /locus_tag="VHV99_18825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX3637500.1"
                     /translation="MHKTFIFMLFALFGLAGLVAEAHAQADYKIVTGPERGTYIQIGQ
                     DLAKWVADPIGMGLDVLASKGSAENVQRMRYEPGVKFALVQSDVYQAYLDMAKAGNAE
                     AGKVIQPLRLIMPLYNEEIYFVVRSDSPLNYIHEIKNKNISVGLIGSGTAQSATTLYR
                     LMFNEPIPDQYAQHYSNEDALAKLIVRKLDVAVIVAGQPAKLFQDLNADLLGQIKLLK
                     LDPSAPETARAEQTYFPATIRTSSYPNWIKEDSPTLTVKAFLVTYDYNLRGTVGAMTQ
                     FADSLCAHFDELQANGHPKWKQVKMELPPLSRGWKYYPPIEKRLRACIRKMASTGSGD
                     SATPVTQRAPKRSCTDAEKVLMLCNNE"
     gene            complement(9433..>11694)
                     /locus_tag="VHV99_18830"
     CDS             complement(9433..>11694)
                     /locus_tag="VHV99_18830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008352950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HEX3637501.1"
                     /translation="PEYEVLDTGVFEDNAYFDVHVEYAKNTPEDIVMRVTVENRGDRS
                     ASLDLLPQIWARNTWSWKPSKKPTLALQSDAKGSRVLAQSDGHEPLVVTAEVRGDATV
                     DWLFCENDTNVRRLFGSEGAGPFKDGFNDYIVQGEECAIRRDTGTRAAAHVHLEFAPN
                     SRTVVTMRWRPASDADRDRQALDIDALFARRIAEADAFYAGLQHEIDDADQRLVQRQA
                     LAGMLWSKQYYQFDVTRWMDGDPLQPTPPASRKRGRDADWRHLCNADIVSMPDKWEYP
                     WYASWDLAFHAAAFALIDPAFAKKQLLLLVKDRYQHPNGQLPAYEWAFGDANPPVHAW
                     ATWRVYEIDRAITGKSDRDFLELVFHKLLLNFSWWVNRKDADGRNIFQGGFLGLDNVG
                     IFDRGAPLPTGGRIDQSDGTAWMAAYALDLMRIALELAVANHVFVDIGVKFFEHFLYI
                     AEAVSCSDSCDTGLWDERDEFFYDVLQLPDGTSVPMRVRSIVGLIPLFAVHVLEEHLH
                     GQLPGLRDRLTWFLEHRPDLAKLVSRWNEPGKGNSLLLSVLRGHRMKALLRRMLDESE
                     FLSDYGVRALSRVHRDNPFVLEHNGDRFCVAYLPGESDSRVFGGNSNWRGPIWMPVNY
                     LLIESLYEFHRYYGDDFRVEYPTGSGQKLSLSDIADELARRLTRLFLKGKDGLRPVMA
                     AYPQLQADPRSEDLVLFHEYFHGDTGRGVGASHQTGWSGLVALLLQPRVSVIAQNLPV
                     GGEVHPEVELATK"
BASE COUNT         2243 a         3815 c         3542 g         2094 t
ORIGIN      
        1 tttctatggc caccccgacc taccgcggcg tgtttccggt cgcgccgacc gtcttcgaca
       61 cagccggccg cctcgacctg gatggccagc gacgtgcgat cgacttcatg atcgacgccg
      121 gatcgcacgg cctgtgcatc ctcgccaact tctccgaaca attcgcgctc accgacgacg
      181 aacgcgcctt ggtgcaaaaa accgtgctgg aacacgtggc cggccgcgtg ccggtgatcg
      241 tgaccaccac gcatttcagc agcgatgtct gcgcggcacg cagccgcgcc gcgcaggatg
      301 ctggcgctgc gatggtcatg gtgatgccgc cgtatcacgg tgcgaccatc cgcgtgggcg
      361 aacgcggtat ctatgaattc ttcgaccgcg tgtccgacgc cgtcggcatc ccgatcatga
      421 tccaggatgc gccggttgcc ggtacgccgc tgtccgcttc gttcctggcg aagctcgcga
      481 aggaaatcgc gaacgtgtcg tacttcaaga tcgaaacggc gcaagcggcg tcgaagctgc
      541 gcgaactgat cgaactcggc ggcgacgcgg ttatcggccc atgggatggc gaggaagcca
      601 tcacgctgat tccggatttc gatgccggcg caaccggcgc gatgaccggc ggcggttatc
      661 cggacgggat tcgcaagatt gtcgacgcct atacatccgg tgacacggaa ggcgccgcag
      721 cgctgtatga gaaatggctg ccgctcatca actacgagaa ccgccaatgc ggtctcgcag
      781 cctgcaaggc gctgatgatg gaaggcggcg tgatccgctc cgacaccacg cgtcacccgt
      841 tcgccgcgat gcatccctcg acccgcgccg gcctgatgaa agtcgcgcgc cgcctcgatc
      901 cgctcgtgct gagctggggc aagtaacgcg gggcaagtga caagtccgca cgcctaatta
      961 caccaacagg attcgaacca tcatggaaat ttccagcgat ctgctgatcg gcaaacaatc
     1021 tgtccacggc acaaacggct caatcaaggg cattaatgcg gcgacaggcg agccgctcgc
     1081 gcccgccttc ggcggcgctt cgctcgacca gttggagcaa gcctgcgcgc tagcatggca
     1141 agcatccgat atctaccgcg aactgccgct tgaaacccgc gcgacattcc tggaaacgat
     1201 cgcgcagaac atcctcgata tcggcgatgc tttgatcgac cgctgcgtgg ccgaaagtgg
     1261 cctgccgcgc ggccgcattg aaggcgaacg tggccggacc gtcggccagt tgcgcatgtt
     1321 cgcggcggtc gtgcgcgaag gcgatttcct tggcgtgcgt atcgacccgg cgcaacctca
     1381 gcgccaaccg ctgccgcgcg tcgacctgcg gctgcgttat gtgccgctcg gccccgtcgc
     1441 cgtgtttggt gcaagcaact tcccgctggc gttctcggtc gcgggcggcg acaccgcctc
     1501 cgcgctggcc gccggttgtc ccgtgatcgc gaaggcgcat tcggcgcacc cggggacgtc
     1561 ggcgctggtc ggacgcgctg tgcaaaaagc cgtcgcggac tgtggtttgc cggaaggcac
     1621 gttctccctg ctcttcgata gcggccgcga catcggccag ggtctcgtct cggatcgtcg
     1681 catcaaggca gtcggcttca ccggctcgcg cggtggcggc accgcgctga tgaagctggc
     1741 ggccgcgcgt cgcgaaccga ttccggtcta cgccgaaatg agcagcatca acccggtcct
     1801 gctgttcccg cacgcgctga aaaatcgcgg cgcggcgatc ggcaaggcct tcgcaggctc
     1861 gcttgcgctc ggcgccgggc agttctgcac gaaccccggc ctggtgctgg caatcgatgg
     1921 tcccgagctc gatgcgttca tcgaagaaac cggtgctgca ttgagcgaaa cacccgccgc
     1981 cacgatgctg acacctggta ttcacaaggc ttacgaatcg gctgtgaaca agaccgcgga
     2041 acacggcgag gtcaagacac tcgcgcgagg ctgtgcggcg aaaggcgcga cgcaggggca
     2101 agccgcgttg ttttcgacca ccgctgcggc gtttcgcgct aatccggaat tgcacgagga
     2161 aattttcggc gcggcttcac tgatcgtacg atgcccggat ctggcgacgg tgctcgagct
     2221 cgtcgagcaa ctggaaggcc agttgacagc ggcattgcat atcgatgaag cggattacga
     2281 cgatgcacgc cgtttcctgc cagcgctcga acggcgcacc ggacggatcc tcgtgaatgg
     2341 cttcggtaca ggagtagaag ttggacatgc catggtccat ggcggcccct ttccttccac
     2401 cgccgacggc cggtccactt ccgtgggcag tctcgccatc gaccgcttcc tacgcccggt
     2461 gagctaccag gacatgcccg aaggcttgtt gccggacgcg ttgaaaatcg ccaatccgct
     2521 cgggttgaac cggcggctgg acggcaaact gcaattatcg aagtaacgag aagcaaggat
     2581 ctggagcggc gggcaatcag gcttcgaggc agcaggtcag gctgacctcg aagtattcgt
     2641 agctgcgctg gatgctgcgt ttgacttcgc ggacgtcggc catgcgcggc gtgcggccca
     2701 gcatggtggc cactatatcg agcgcttccc acggatgcgc gtcgtcataa tgggtgtgca
     2761 tcctgagcca gcgcatggtc ttcttgcgga ccggttcagg gtagctgtgc gcgagcgtgt
     2821 cgcggctgca gagtagcgtg gaccattctc cggtcacccc ttcaacggca aagttagtcg
     2881 ccgctattga cgcatgcagc gggtccgcat tgctgctttt ccagcaccag tggctcagtg
     2941 catacgcaag cgcgggcgct tcgccggcct gcaaatcttc gcgcgttaca tcatgggccg
     3001 cggcccagtc gacccaatag tcggcgtgat tctgctcgac gcggatatta cgcgtgagat
     3061 agcgccgcgc catgcgcgaa cccggcgttt cttcgggtcc gatcttctgc aggttcatgc
     3121 ccatgtactg cggaaactga ttgatcaccg gccagccgtt gatcaggaac gcacgcattt
     3181 caagcgggcc aagctcaccg tcgcgcaatg accgaaaaag cggatgagac gtcacacggt
     3241 tttttgccgc gccggtttcc aacaaaactt cctgcaacca aagtgggtag cttgctgtgt
     3301 tcttgaggtc gccctctctt gtaaacccac cccccatcac gcctccttag ctaatgtcag
     3361 gacggccggc tctgtccaaa gcgttggccg tcctatgtaa tacccttgcg cataatccac
     3421 cccaagggca tgcagcgccc gcaggatccc ttcgctttcc acatattccg caatggtccg
     3481 gcatttcatc gaatggccca tttcgttgat ggcagcaacc atctcgcggc tcaccggatc
     3541 gagcaccatg tcggtcacga aactgccgtc gatcttcaaa tactctacag gcagatgctt
     3601 caggtaaccg aacgacgaca tgccggcgcc gaagtcatcg agtgcgaaat gacagccgag
     3661 cgcattcagc tcgtgcatga agcgcgccgc cgatgccagg ttggcaatcg cggtggtctc
     3721 cgtaatttcg aagcagatca tgccagggga gacgcccgtg cgctgaaatt gctcgcgcac
     3781 gaaacccagg aaacgttcat cgcccactga ggcaccagac aggttgatcg catattcctt
     3841 gaagcgcttg tgacgggtac gcgaaagcat atcgaaggca ttgcggatca cccaccggtc
     3901 gatagccgtc attagtccat agcgctcggc ggcaggaatg aagagcgtgg gcgcgatcac
     3961 gccgttgatc acgcccgggt caagcatgcg caacagcaat tcggcgcgct gcccttccgc
     4021 ggaggcgggc aagccgtcgc ccacggccac gatgggttgc acgtagaggc agaagtcatc
     4081 gtgttcaagc gcctgcttga gacgtcgtcc ccacgatacc tcgctcgcgt ggcgcgcaag
     4141 ttcgcggtcg ccgaggtccg ccagttgcac ccggtcgcgg ccgcgttcct tcgccatgta
     4201 gcaggcaata tcggcgagcc gcatggcctc ttccacgctc atttccgtgt tgcgcaggtc
     4261 aaccacgccg acgctcacgc tggtggtgaa cggctgcgcc tcccagttga agacgaagtc
     4321 attcaggcac gcgcgcaggc gttctgcgac ctcctggatg cccggggtgt cgtaaccctg
     4381 cagcagcacg ccgaactcgt cgccgcccag ccgcgcgagc gtgtcgtcgg gttgcaggct
     4441 ggcttcgaag cgcaacgcga cttcacgcag catggcgtcg ccggccgcgt ggccgcaggt
     4501 gtcgttgacc accttgaagc ggtcgagatc gagaaacagg agcgccgatg ccgccggcag
     4561 ccacgacgcg acctcgctcc agcgcgtacc gagcgcgtgt tgcaggcgcc gctcgaattc
     4621 ggcgcggttc acgagcgccg tgagcccgtc gtgcgtggct tgccatgcaa gactttcgat
     4681 atattcgtac tcgcgggtca tgttgcgcag gatcagcacg aaaccagatt ccgcgttggc
     4741 catgttcgga cttgcaccgg ccgcgctgat ccgcgacgtt accgcctgca cgcgaatctc
     4801 cgcgccgtca cgccgctgca gcagcatttc ggcggattcg ccgtcgggac gtgcgcctgc
     4861 ccgggcgtcc tggatcgggt tgagcggctg gcggctatcg gcgtgaagca acctgaaagt
     4921 ctggaccagt ggccgcccga gacagtcatc cgccagacgt ccggcgatcc gctcggccgc
     4981 cgggttgatg aattcgatat gccccgacgg ccccgtcgcg atcacggcct cgccgatcga
     5041 accgagcgtc gccttcgcgc gcgcctcgct ctgcttgagc gcagcttcaa tgcgcccgcg
     5101 ctgcctgatg agccgccgca catgcaccgt tgagaggcac acgagcaaca gtgcggcgac
     5161 caggtcgacg gtcagcaata acgacgctgt cttgcggaat gcagtaccta atacctgtga
     5221 gaaaccccat gacagcggcg caatagtcgc gttgatggtc catatctgcg atttataggc
     5281 actcacgagc gccacgggca attcaccggc cgcgacccgc gcatgtaact cgttgccaac
     5341 ccgctccagc ttcgccattt cggcgtcgcc gcgtgaccag taaccgaccg cctcgctgaa
     5401 ataactgacg tggcgaaaca tcttgaaggt ccagatcacg tcgctgacgt cgtcgggatg
     5461 tacgccgctt gcaatcagcg cccggcgtgc ggtgaccgga tcgggcttgc tctggtcgag
     5521 cgccaaacgg gcagtgcgca ggttgcgcgg gccttccatg gcggtcagat attgcttgta
     5581 ggtactttcg gagccggtct cggcgtacag attcagatag aaagccgcat ctttctggcc
     5641 cttcgaccag atgctttccc cgccaatgta ggcccgcacc gacgacatga tgtcgatact
     5701 gaaaatggcg agcgaaacca ggacaagaat gacgaccaga aacggccagg tgatccgtat
     5761 gagctgcgca ttctcgccct tagacggcat gtccatcgat atgtcccttc tccagacccg
     5821 atgccggtgt gaactgccgg ggtcatgcgg cgagcatcgg gtggtgagtc atttgtattt
     5881 ttggcggctt tcggggtgtt gcgcccagcc tctcgcagcg cgcttacatc ctaccaaatt
     5941 gcgagtctgg ctcaaattta ttacgcagtg tgctattcag cgcaaacacc agaaaatatc
     6001 gggttccggc tgttgttttc gaatccgcaa cggcctgttt cgaagcgcca caaagcgcca
     6061 tattcggaca gaacctaggc tcctgccgac gaaaccaccg gccgctttta cctgttcgcc
     6121 agcgccttga ccggtggcgc caaagcaggc atttaagcgg cgtggcgcaa gcctcgcgtg
     6181 atccttcccg agcgtgttga cgccgccgcg gagatgtcac atattggttc cagctccctc
     6241 gatgactgtc cagcttaggg agtccgaagc atttcgtcat acgagtgact gtattgtccg
     6301 attaaaccgg acacgcaagc gggaaggaca acccttgtgt cccgggaggt ttttagcatg
     6361 gcccgtctct tctttcccac atccgccggt tccgtgacct gcaagcgttg cggcaacgac
     6421 attccgagcg atgccgatac ctgtccgcat tgcggcgccg atcatggccc gggcttcggc
     6481 gcggcgaaaa ctgccgcgct tgcaacgggg ctgcgcttgc ccttcggcgc gcgtcgtgaa
     6541 gccctgaaca tcccgttgcc ctacccgagc gtgccggaag agtccgaact ggccggcacc
     6601 ggcgagcagc gctgggacat gtcgaaaacc atcacgctgg gcgccgtggc gctggctctg
     6661 gtagccggcg gcgtgatcta ttcgcagcac ggcagcagca gcggtgacga caccacgcgt
     6721 tccgagctgc cggccgggca tagcgcgtat ggcgccatcg atatgaaaac cgcgcacgac
     6781 gcgcctgcgc gcccagctgc gcccgcagtg cccccggcca ggtcagcggc ggcggccacg
     6841 gccactaccg ccaggccggc gcaaacggcg gcagcgccct ccgttgctgc ccgcgaagcc
     6901 ttgcccaccg acacctcctc cgctcgcgcg gcaacggcgc tggccagcac aacggacaac
     6961 ctgcaggccg cgcgtgacgc gatcacgcgc ggcgacctga ccacggcgcg ccggcgcttt
     7021 tcgaagatcc cggcgtcgca actgagcgcc gccaatgtgc agcgaaccct ggcagacctc
     7081 accggcctgg aacacgcacg tgacgagatg ctgcaagtgg cgcgcggttg cgaagcgacc
     7141 ggctcgtggt tgtgcgtgcg ccagaacgcc cgtgacgtgc tgacgatcga tgccagcaat
     7201 gcggaagcgc aagcgctcgt cgagcacgcg atcgcccgct cgggctggct caataaaccc
     7261 acgtccgctc ccgcgaccac agcggcgcat ggcgctcgcc acaacaacgc gcagacggct
     7321 gcagcggcgc cggcgcccgc agtgccagca cccatgtatg tgcccgggcc gcgcgtcgcg
     7381 gccacacctc cgcccgcgtc gacgcacaag cccgccgtca ccgtctcggc cgccgaacat
     7441 gcgcgcgccg tcgccgcgat gggtgccccc acgcctattc cgacgcccgt agcggccgca
     7501 ccggtcgcac ccacggttgt cgccaccatg cgctcgcctg tcacggcgca ggccgttcca
     7561 ggcacgggct cgcaggctcc gctcacgtcg tcgccgacca cctcaccgca gccgcggcaa
     7621 caggccgcgc cggctcgtcc cccagtaccc tcagcacccg cgtttgtccc gatgccggac
     7681 ggaccggacg ccatgcccgc caccgcaccg gtacgcagct cgccggcggc cgccacggca
     7741 cgcgcaccag cgcaacccga acccgagcct gcgaccgctg cccaggttcc aggcgatacc
     7801 gcctcgccgc ccgtggtacg ccaactcgca ccgatcagcg aagccgttcc ggtgcgggcg
     7861 ggcattgtgt cgcctcctgt cgccgccaat ggcgcacccg caggtgtgca aaacatcact
     7921 cgttcaccat cgcccgcggt tcatcgcacg ctcgcgtcag gagacaacgc gaccagtgcg
     7981 gcgcaaccgc atgcgccggc cttcaatacg accaattctg acgacgaaga gcgcgcgatc
     8041 ctggagtcgg gctggagcaa gagcaagtcg tctgcacaac gctcgacacc gcagtagaag
     8101 gtacgcagag gcacgcggca gaggcacccg ggagacaagg aacaggagac aaccggaagg
     8161 agacattgag gaggttgaag gccgcgccgg gagacgcagg ccaccgtgat ccatacgtgt
     8221 aatgaaagtt ggccaacaca ttaaaacgac tcacacgaca tcatgcacaa gactttcatc
     8281 ttcatgctgt tcgccctctt tgggctcgct ggcctcgttg cggaggctca tgcgcaagcc
     8341 gactacaaga ttgtcaccgg gccggaacgc ggcacttaca tccagatagg ccaggacctg
     8401 gcgaagtggg tcgctgaccc gataggcatg ggcctggatg tgctcgcgtc caaaggctcc
     8461 gccgaaaacg tgcagcgcat gcgttacgag ccgggcgtga aattcgcgct cgtgcagtcg
     8521 gacgtgtacc aggcttacct cgatatggcc aaggctggca acgcggaagc aggcaaggtc
     8581 atccagccgt tgcggctcat catgccgttg tataacgaag agatttactt cgtagtccgg
     8641 tctgattcgc cattgaatta catccacgag atcaagaaca agaacatcag cgtggggttg
     8701 atcggcagcg gcacggcgca atcggcgacc acgctttaca ggctgatgtt caacgagccg
     8761 attcccgacc agtacgcgca gcactacagc aacgaggacg cgctcgcgaa actgatcgtg
     8821 cgcaaactgg atgtggcggt gatcgtggcg gggcagccgg ccaagctgtt ccaggacctg
     8881 aacgcggacc tgctgggcca gatcaagttg ctcaagctcg atccttcagc gcctgaaacc
     8941 gcgcgtgctg aacagaccta tttcccggcc accatccgca cgtcaagtta tccgaactgg
     9001 atcaaggaag actcgccgac gctcaccgtg aaggcgtttc tcgtcaccta cgattacaac
     9061 ctgcgcggca ccgtcggcgc gatgacgcaa ttcgccgact cgctctgcgc gcacttcgat
     9121 gaactgcaag ccaacggcca tccgaaatgg aagcaggtga aaatggaatt gccgccgctc
     9181 agccgtggct ggaaatacta tccgccaata gaaaagcgct tgcgggcgtg catccgaaag
     9241 atggcatcta caggcagcgg cgactcggca acgcccgtga cgcaacgcgc gccgaagcgc
     9301 tcatgcaccg atgcggaaaa agtattgatg ttgtgcaata acgaatgacg cggacggcgt
     9361 aacagggctg gcggaagcag gcgggctgaa cccgcccgcc tactccgtta cttcgttact
     9421 tcgttatttc ggttatttcg tcgccagttc cacttcgggg tggacctcgc cgccgaccgg
     9481 caggttctgc gcgatcacgc ttacgcgcgg ttgcaacagc agcgcgacca gtccgctcca
     9541 gcccgtctgg tgcgaggcgc ccacgcctcg cccggtatcg ccatgaaagt actcatggaa
     9601 cagcacgagg tcttccgagc gcgggtccgc ctgcagttgc ggatacgcgg ccatcaccgg
     9661 gcgcaggccg tccttgccct tcaggaacag gcgtgtcagg cggcgcgcga gttcgtcggc
     9721 tatgtcgctc agcgaaagct tctggcccga tcccgtcgga tattccacgc ggaaatcatc
     9781 gccgtaataa cgatggaatt cgtatagcga ctcgatcagc agatagttga ccggcatcca
     9841 gatcggcccg cgccagttgg agttgccgcc gaacacgcgc gaatccgatt ctcccggcag
     9901 atacgctacg cagaagcggt cgccgttgtg ctcaagcaca aacggattgt cccgatgcac
     9961 ccgcgacaac gctcgcacgc catagtcgga caggaactcg ctttcgtcga gcatgcgcct
    10021 gagaagcgcc ttcatccgat gtccgcgcag caccgacagc agcaggctat tgcccttgcc
    10081 cggctcgttc cagcgcgaca ccagcttcgc gagatcgggc cggtgctcga ggaaccacgt
    10141 caaccggtcc cgcagccccg gcaactgacc atgcagatgc tcttccagca catgcaccgc
    10201 gaacagcgga atcaagccga cgatcgagcg aacgcgcatt ggcacgctgg tgccgtcggg
    10261 gagttgcagc acgtcgtaga agaactcgtc gcgttcatcc cagaggccgg tgtcgcacga
    10321 atcgctgcaa ctgaccgcct cggcgatgta caggaaatgc tcgaagaact tcacgccgat
    10381 atcgacgaat acatgattcg cgaccgccag ttccagcgcg atccgcatca ggtcgagtgc
    10441 ataggcggcc atccatgcgg tgccgtcgga ctggtcgata cggccgcctg tgggcagtgg
    10501 cgccccgcga tcgaagatgc cgacgttgtc cagccccagg aaaccgccct ggaaaatgtt
    10561 ccggccgtcg gcgtccttgc ggttcaccca ccacgagaaa ttcagcagca gtttgtggaa
    10621 tacaagctca agaaagtcgc gatccgactt accggtgatc gcgcgatcga tttcatagac
    10681 gcgccatgta gcccatgcgt gaaccggcgg attcgcgtcg ccgaacgccc attcgtaggc
    10741 gggcagttgc ccgttgggat gctggtagcg atctttcacg agcaacagca attgtttctt
    10801 cgcgaatgcc ggatcgatca gcgcgaacgc ggccgcatga aacgcaagat cccacgatgc
    10861 gtaccacggg tattcccatt tgtccggcat cgagacaatg tccgcattgc acaggtgccg
    10921 ccagtcggca tcgcgaccac gcttgcggga ggccggcggt gtcggctgca acggatcgcc
    10981 gtccatccag cgcgtgacgt cgaattggta atactgcttc gaccacagca tgcccgccag
    11041 cgcctgacgt tgtacgagcc gctggtcggc gtcgtctatt tcgtgctgga gacccgcgta
    11101 aaaggcatcg gcttccgcga tacggcgggc gaacagcgcg tcgatatcaa gcgcttgccg
    11161 gtcacgatcc gcatccgacg ccgggcgcca gcgcatggtc acgaccgtgc ggctgttcgg
    11221 ggcgaactcg agatgcacgt gcgcggcggc gcgcgtgccg gtatcgcgcc ggatggcgca
    11281 ttcttcgccc tgcacaatgt agtcgttgaa gccgtccttg aacggtcccg ccccttcgga
    11341 gccgaacaag cgcctgacgt tggtatcgtt ctcgcagaac agccagtcga ccgtggcgtc
    11401 gccgcggact tcggcggtca cgaccagtgg ctcatgaccg tcgctttgag caagcacgcg
    11461 actgcccttc gcatcggatt gcaaggcgag cgttggtttc tttgaaggtt tccacgacca
    11521 cgtattgcgc gcccagatct gcggcagaag gtcgagcgat gcggagcgat ccccgcgatt
    11581 ctcgaccgtc acgcgcatca cgatatcttc aggcgtattt ttcgcatatt cgacatggac
    11641 atcgaaatac gcgttgtctt cgaacacgcc cgtgtcgagc acttcgtact ccgg
//
LOCUS       DATASH010000710        12099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2489602,
            whole genome shotgun sequence.
ACCESSION   DATASH010000710 DATASH010000000
VERSION     DATASH010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 12099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12099
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2489602"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..1389)
                     /gene="tssK"
                     /locus_tag="VHV99_18835"
     CDS             complement(22..1389)
                     /gene="tssK"
                     /locus_tag="VHV99_18835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HEX3637502.1"
                     /translation="MNDHHSPLKNGSTARSKVVWSEGMFLRPQHFQQLERYVEAYVQQ
                     RCLASHAAFWGFQSVEVDHAALALGKVALASAQGVLPDGTPFLLTEPDELPAPLDIPA
                     DAKDLTVLLALPRRRAGADDTIFEEHAGSLARHAVYSRDIADSNAVALGPAALQLARP
                     RMRLMLATELGSQWHGIGLLRVLERRSDRQLVLDKHYIPPMLSADQHPVLAQYLRELH
                     GLLEQRSEALAQRQSRPGRGGVAEVSEFLLLALINRAQADTWHALHMPHLHPERLFQQ
                     WLRLAFELSTYTAEERRPALYPSYQHDDLQGSFGPLMAELRRALSTVLEQNAIALELQ
                     ERAHRVWVAQIPSVELLHHAGFVLAVHADLPAETVRTRYPAQVKIGPVERLAELVHLQ
                     LPGIAMRSLPVAPRQIPYHAGYHYFELDAGGELWKQLEQSGGLALHVAGDLPGLTMEC
                     WAIRG"
     gene            complement(1436..1879)
                     /gene="tssJ"
                     /locus_tag="VHV99_18840"
     CDS             complement(1436..1879)
                     /gene="tssJ"
                     /locus_tag="VHV99_18840"
                     /inference="COORDINATES: protein motif:HMM:TIGR03352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HEX3637503.1"
                     /translation="MLALGAALLAGACASSAKPEARAWDIYLEAAANVNPDGHGRPAP
                     ILIGLYTLKSTAVFEASDFPSLQERAKAVLADDLVSFEQIILLPGERKLIQRSAEPGV
                     RALGVVAGYRELDRTAGKPPTHSPPTTRAACFHSGRPLTSGLPCK"
     gene            complement(1955..2344)
                     /locus_tag="VHV99_18845"
     CDS             complement(1955..2344)
                     /locus_tag="VHV99_18845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010112406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4150 domain-containing protein"
                     /protein_id="HEX3637504.1"
                     /translation="MVMVNSSAGGANDAVCVNLTPPVGVPVAYDNNAQRPQAIPNVSN
                     ILIAAAPAHNLATIIPVTSGDAPGSMGGVISGTVCASSRNINGANTVLLHGMPTTRMT
                     DPTQQNATNAIGTGTSPSQTHILNLAG"
     gene            complement(2354..2899)
                     /locus_tag="VHV99_18850"
     CDS             complement(2354..2899)
                     /locus_tag="VHV99_18850"
                     /inference="COORDINATES: protein motif:HMM:NF023482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3540 domain-containing protein"
                     /protein_id="HEX3637505.1"
                     /translation="MLVTGDDGALLTCKRAFSCLITPAIRDRVAVCGSGPRHGYVAAI
                     LERAAGSETCIRVEGDLRIDASGDVRVSAAREIALEGERLAVATHDAEFTATKSTLVC
                     DELEGRIGLVRLLGKTIEAVVERIVQISKTSFRSIDTVDHLRAAHIDYAASESVRLHG
                     KNTLLSAEHLSKLDAEQIHLG"
     gene            2786..3244
                     /locus_tag="VHV99_18855"
     CDS             2786..3244
                     /locus_tag="VHV99_18855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637506.1"
                     /translation="MPRAAAADCHPVADRRCDQATERALAGEQCAVVAGYEQDVVVQD
                     GDHRADAVMWALGVVGAFGMFRMFCAFSAVDRFRTFNAFDTFDVVRMFDTLNALGMFR
                     MFCALNTSNTSDMLNTFDMLRTFVTRERFDTFDSGVMLRHAPLRCGRCHG"
     gene            complement(3241..4338)
                     /locus_tag="VHV99_18860"
     CDS             complement(3241..4338)
                     /locus_tag="VHV99_18860"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEX3637507.1"
                     /translation="MMSAREQLQQAVRNGEAISNRSIGALALERCDLSGGIFDKVTFE
                     GTRLAQARLAESVFTDCQFNGVDLRGADLSKSVFINGALTGTDLSGSLLDDCRLSGTR
                     ASGTRFSEVRAARLACLHAELHASRFDGAQIDQGFFDETPLRGADFSAAHLQRCFFYR
                     LDLREVGFARTEFDRVVFTESDLSGQRLNGQTFVRCQFIATDLSRADFTSANLTHCNF
                     QGARLDHAMLEHVHAPSAIFFGANVGHARCAHAQLENSIWVDAHAQHSDFSDADLRGA
                     VFQRADAAHARFVRTCLNAADFSHAALAGADFHEAVFARTSFHGAQGAEPATSPGRPG
                     VIAAEPALLAAQEWSRHRDDRRRHTFIKEQP"
     gene            complement(4335..6854)
                     /locus_tag="VHV99_18865"
     CDS             complement(4335..6854)
                     /locus_tag="VHV99_18865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004523545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2169 domain-containing protein"
                     /protein_id="HEX3637508.1"
                     /translation="MKIIKPTPVGILTRTYRQAGNEHLGIAIPLMVTLGNAPRLVGES
                     ELWNTVGSELGGYALDAALPKLHAEFLVAGRAYGQYCNAAHMCRVGIRFAGLDKQLQV
                     SGAREWNGDTPSQPTPFECLALDWPLAYGGPGYADNPRGIGFPTGAPGTAQLLPHIAY
                     QDAPVVTRERGIAPAAFTPIAADWPQRSALYGEMDERWLKEDCPGFPRTMHADYFNVA
                     PRDQQFPHLDSLPPGAAYELTHMHPERPTLAGALPPLHARCFVQRTRESDLTEVGMRL
                     TTVWFIPHRERAIMIFHGAARVQEFDAQDVACLLLAVECHNEPRSDAHYRRVFDLRMD
                     SKRGVLHALRDLDLAPQSMLAAAEDADGDGDAQAGPQGSALQRNLQQRAREQASQAGA
                     AGVVISAPEVLATRPPRLDELAEFVERNEKLAETQLAALEQTRQRIEAEHPELTASRS
                     AAARRGPPQSFASNRAQHPARPEMLEQEREAERKLRASYLQSAHYQEAAARLDTASSA
                     ALRERVAAGHGRGESLAGLDLTGADLSGMNLRDVDLAGALLESADLSGTDLTGANLRD
                     AVIARATLAGTTFARADLSGANLSLADCEGTDFSEAVLDRCLFERAVWRNCTARRASA
                     RRVQFSGCRFEAVDFAEATLSELVFIEQTLTDVTLHAATIRKLAFIQCRLDRVAFTSA
                     NIEGFGFVETVAQQIRFNHARLHKACFAKDTVLDRADFSQASLTEVNLRHTQARNARF
                     GGATISQCDFSDAGLQQADLSDAKFDGGYLVRTDLSGASLAGADLIGAQMRRAMLHDA
                     DFDEANLFRANLAEASIDASTRLDHAYLEQTVFHPLRRASA"
     gene            complement(6878..9061)
                     /gene="tssI"
                     /locus_tag="VHV99_18870"
     CDS             complement(6878..9061)
                     /gene="tssI"
                     /locus_tag="VHV99_18870"
                     /inference="COORDINATES: protein motif:HMM:TIGR03361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HEX3637509.1"
                     /translation="MNHIIIAHTPLDPQMLRFRSLRGEESLSALYEFNVELVSPSASL
                     DMNALLGKPLTLHIVTLNGSTRYLGGQIVRCEFAGRETDTPRTYIYAVTLRPWLWYLT
                     KTTDCRIFQNHTAVEILREVIGKYGFPVEERLLASYRSWDYCVQYQESDFNFVSRLME
                     QEGIYYYFRHEAGQQVLVLADDASAHDPLPEHAMLPYQSPDRLALPREEGIDEWRPAT
                     QISAGAFAVDDYDFRKPRADLSQMRAQSSSAEHGDYARYAWQGGYIEAEQGEHYVRMR
                     LEEEQAANARVRAHTRLREAAPGYRFTLQDCPRQAENREYLLVSVVYRLQEGGYASGS
                     SHAQYDFDLVAQPASLPFRAPSVTPVPKATGPQTAVVVGPAGQDIWTDQYGRVKVQFR
                     WDRYGKRDENSSCWIRVSDAWAGSGFGSIHIPRVGQEVVVDFLNGALDRPVITGRIYN
                     ADQMPPFALPEAATQSGFVTRTPGGSSANANMLRFEDKAGAEQIALHAERNYDVSVEN
                     DATRQVGRNEAVNVSNDATRGVGRDYTVSIGNDYAASITSNQSRKVGNNSSAEVANDE
                     THTIGRNYSLNVSNDSTHTVDRKHTLTVAGDMRHTVGSDHSLSVASNSTTSVGADSTS
                     SVAANSTSTVGGNSAVTVSGSSTSIVIGLQTGITTAQLFVTGSIVTATGSSVGATGSS
                     VSATGSDVGATGSSVSTTGSSVSSTGSSVSATGVSFSTVGFSVTF"
     gene            complement(9072..10157)
                     /gene="tssG"
                     /locus_tag="VHV99_18875"
     CDS             complement(9072..10157)
                     /gene="tssG"
                     /locus_tag="VHV99_18875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HEX3637510.1"
                     /translation="MSNAIAPSSRVPHASSMLAHFWQCVADAPYKYDLYNLLRWLDAR
                     AAAPAPLGRAAHPADEPLRIGQLPSLAFAPATLAAASVPEGAKPRVLIHSFGLFGPNG
                     PLPLHLTEFVRERARHHDDHALSAFADLFHHRLILLFYRAWADAQPTVSLDRGEGGRF
                     DRYLASLIHLPRREARRPDTLSDHARYFMAGHLVRQTRNAEGLRSILRACLGVPVQIV
                     EWLPQWLPVEAPRRTALRTHANTRRLGADAMLGAAVRDAQTKFEVRLGPLSREQYAAL
                     LPGTPLSQQLTDLVRQYAGVEWSWDVRLLMAADQVAGIALGAGRPLGCASWLGTRTST
                     LTADDLVYQPETRDTRNTRAASAQSGL"
     gene            complement(10154..12061)
                     /gene="tssF"
                     /locus_tag="VHV99_18880"
     CDS             complement(10154..12061)
                     /gene="tssF"
                     /locus_tag="VHV99_18880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004190509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HEX3637511.1"
                     /translation="MDARLLDYYNRELAYLRELGAEFAAEFPQAAGQLGMRGIEVSDP
                     YVERLLEGFSFLTARIQLKMDAEFPRFSQRLLDVVYPNYLAPLPSMTIVAVQPNLREG
                     SLLSGYTLPAGTSLRGNLAPGEQTACDFSTAHDVTLWPLRITEVKLGGVPADLPLPHG
                     QLTSRVRSALHIQLEASGVGNLRELPLDSLTFFLSGPDNQALPLLEQVLGHQLGVICH
                     GPSVSGNTSHTGSACSLLDGEAIRQEGFDAAQALLPYGSRSFQGYRLLHEYFAFPQRF
                     MFFSIGSLQRALQNIDGTTFSLTVLLDEASPELERSVDLRSLALFCTPAINLFPKRTD
                     RIAITNQRYEHHVVVDRTRPLDYEIYGVEKVVGHRQSDGGHCEFHPFYGGLSHDHADR
                     GRYFSMRREARLVAPRDHAASRAARSSTRSAYAGSEVFISLVDQHEAPFSDDLRHLSV
                     DAMCTNRDLALLMPTGSTSDFSLRTSAPVAAIQALRRPTPPQPPLAENEITWRLIKHL
                     GLDYLTLSDLDSVQGAQALRELLELYASAADAAVRPQIDGVRSVRVEPVCRQLPQAGP
                     PMFGRGVAVSLLLDETSFAGASTYLFGAVLEQFFARHVSLNSFSELAVATLQRGLVKR
                     WPPRVGSRPTA"
BASE COUNT         2097 a         3715 c         4070 g         2217 t
ORIGIN      
        1 ctttctttcg catacatgag atcagccgcg aatggcccag cattccatcg tcaggcccgg
       61 cagatcgccg gccacgtgca gcgcgaggcc gccggactgt tcgagttgct tccagagttc
      121 accgccggcg tcgagttcga aatagtgata gcccgcgtga tacgggattt gccgcggcgc
      181 gaccggcagg ctgcgcatgg cgatgcccgg caactgcagg tgcaccagtt ccgcgagccg
      241 ttcgaccggg ccgatcttca cctgcgccgg ataccgcgtg cgcacggttt ctgccggtag
      301 atccgcatgc actgcgagca cgaagccggc atgatgcaga agctcgacgc tcggaatctg
      361 tgccacccag acgcggtgcg cgcgctcctg caattcgagt gcgatggcgt tctgttcgag
      421 aacggtcgac agtgcgcggc gcaattcggc catcaagggc ccgaaactgc cttgcagatc
      481 gtcgtgctgg tagctggggt aaagcgccgg ccggcgctcc tcggccgtgt aagtggacag
      541 ctcgaacgca aggcgcagcc attgctggaa caggcgctcc ggatgcagat ggggcatgtg
      601 cagcgcgtgc cacgtatcgg cctgcgcccg gttgatcagc gcgagcagca ggaactccga
      661 aacctcggcc acgccgccgc gccccggccg tgactggcgc tgcgcgagcg cctcgctgcg
      721 ctgttcgagc agcccgtgca attcgcgcag gtattgggcg agcaccggat gttgatccgc
      781 actcagcatc ggcggaatgt agtgcttgtc gagcaccagt tggcgatcgc tgcggcgctc
      841 cagcacgcgc agcaaaccga tgccatgcca ttggctgcca agttcggtgg cgagcatgag
      901 ccgcatccgc ggccgggcca gttgcagagc cgcgggcccc aacgcaacgg cattgctatc
      961 ggcgatgtcg cgcgaataga cggcgtggcg cgcgagtgat ccggcgtgct cttcgaagat
     1021 cgtgtcgtcg gcgccggctc gtctgcgcgg cagagcgagg agcaccgtga ggtctttggc
     1081 gtcggccgga atatcgagcg gcgcaggcaa ttcgtcgggc tcggtcagca gaaacggcgt
     1141 gccgtcgggc aacacgcctt gcgcgctcgc gagcgccact ttgccgagcg ccagcgcggc
     1201 gtggtcgacc tcgacgctct ggaaccccca gaacgcggca tgcgacgcga ggcaacgctg
     1261 ctgcacatag gcttcgacat aacgttcgag ttgctggaaa tgttgcggcc gcagaaacat
     1321 gccttctgac cagacaactt tgctgcgggc ggtgctgccg tttttgagtg gcgaatggtg
     1381 gtcgttcatc ggtgtgccca atccatggtt ctaacgatca ggccgccgct gagcttcact
     1441 tgcacggcaa gccgctcgtg agtggacggc cagaatgaaa acacgctgcc cgagtcgtcg
     1501 gcggggagtg cgtaggtggc tttccagctg tgcggtcgag ttcccgatag cccgcgacga
     1561 cgccgagtgc ccgcacaccg ggctcggcgc ttcgctggat cagcttgcgc tcgcccggca
     1621 gcaggatgat ttgttcgaac gagacgaggt cgtcggcgag gaccgctttg gcgcgctcct
     1681 gcaaggacgg gaaatcgctc gcttcgaata ccgccgtcga tttcaacgtg tagaggccga
     1741 tcagaatcgg cgctggccga ccgtggccgt cgggattcac gttggcggcg gcttcgagat
     1801 agatgtccca ggcgcgcgct tcaggtttcg cggacgatgc gcacgcgccg gccaacaagg
     1861 ccgcgcccag ggcgagcaaa aggcgtaggc gtaatcggag gcgtgcgcgt aggcgtagct
     1921 gtaggcgctt catcgaggtg cgatcatgtg aggcttagcc cgccagattc agaatgtgcg
     1981 tctggctcgg cgatgtgccg gtgccgattg cgttggtcgc gttctgctgc gtggggtcgg
     2041 tcatgcgggt ggtcggcatg ccgtgcagca ataccgtatt ggcgccgttg atgttgcgcg
     2101 aactcgcgca tacggtgccc gagatgacgc cgcccatcga gcccggggca tcgccgctcg
     2161 tgacgggaat gatcgttgcc aggttgtgcg cgggcgcggc ggcgatcagg atgttgctga
     2221 cattgggaat ggcctggggc cgctgtgcat tgttgtcgta ggcaaccggg acgccgacgg
     2281 gcggcgtcag gttgacgcat acggcgtcgt tggcgccccc ggcgctcgag ttgaccatca
     2341 ccatcgtcca gtcctatccc aggtgaatct gttcggcgtc gagcttcgac agatgctcgg
     2401 cgctcagcag ggtgttcttg ccgtgcaggc gcacgctttc cgacgcggcg tagtcgatgt
     2461 gcgcggcgcg cagatggtcg acggtgtcga tcgagcggaa actggtctta ctgatctgca
     2521 cgatgcgctc gaccaccgct tcgatggttt taccgagcag acgtacgagg ccgatgcgtc
     2581 cttccagttc atcgcagacg agcgtgcttt tcgtggccgt gaattccgcg tcgtgcgtgg
     2641 cgacggcgag gcgctcgccc tccagcgcga tttcgcgcgc cgcgctgacg cgcacgtcac
     2701 cggacgcgtc gatgcgcaag tcgccttcca cgcggatgca tgtctcgctg ccggccgccc
     2761 gttcgagtat cgccgccacg tagccgtgcc gcgggccgct gccgcagact gccacccggt
     2821 cgcggatcgc aggtgtgatc aggcaactga acgcgcgctt gcaggtgagc agtgcgccgt
     2881 cgtcgccggt tacgagcaag acgtcgtcgt gcaagacggc gatcaccgtg ccgatgctgt
     2941 tatgtgggcg ctcggggtgg tcggtgcgtt cggcatgttt cgtatgttct gtgcgttcag
     3001 cgcggttgac aggttccgca cgttcaacgc gttcgacacg ttcgacgtgg ttcgcatgtt
     3061 tgacacgctc aacgcgctcg gcatgtttcg tatgttctgc gcgctcaaca cgtccaacac
     3121 ctccgacatg ctcaacacgt tcgacatgct tcgcacgttc gtcacgcgcg aaaggttcga
     3181 cacgttcgac tccggtgtca tgctccggca tgcgccgctg cgctgtggtc gttgtcatgg
     3241 ctagggctgc tccttgatga atgtgtgccg gcgacggtcg tcccggtgtc tcgaccattc
     3301 ctgcgcggcg aggagggccg gctcagcggc gatcacgcct gggcggccgg gagaggttgc
     3361 cggttcggcg ccttgcgcgc cgtggaaaga agtgcgtgca aacacggctt cgtgaaagtc
     3421 cgcgccggcc agtgccgcgt gcgaaaagtc agccgcattc aggcaagtcc gcacgaaccg
     3481 cgcgtgcgcc gcatcagcgc gttgaaatac ggccccgcgc aaatccgcgt ccgaaaaatc
     3541 gctgtgctgc gcgtgtgcgt cgacccagat gctgttctcc agctgtgcat gagcgcaacg
     3601 tgcatgaccg acgttcgcac cgaagaaaat ggccgacggc gcgtgcacat gctccagcat
     3661 cgcgtggtcg agtctcgcgc cctgaaaatt gcagtgggtc aaattagccg acgtgaaatc
     3721 ggcacggcta agatcggtgg cgatgaactg gcagcggacg aacgtctggc cgttcagacg
     3781 ctgcccgctc aggtccgatt cagtgaacac gacgcgatcg aattccgtgc gcgcgaagcc
     3841 gacctcacgc agatcgagtc gatagaagaa gcaccgttgc agatgcgctg cggagaagtc
     3901 ggcgccacgc aaaggcgttt cgtcgaagaa gccctggtcg atttgcgcgc cgtcgaaccg
     3961 gctcgcatga agctcggcat gcaggcacgc gaggcgcgcg gcacgcacct cgctgaaacg
     4021 ggtaccgctc gcgcgcgttc cgctcagacg gcagtcgtcg agcagactgc cggacagatc
     4081 cgtgccggtc agcgctccgt tgatgaagac gcttttcgac agatccgcgc cgcgcaggtc
     4141 gacgccgttg aactggcaat ctgtgaagac gctctcggcc aggcgtgcct gcgcaagccg
     4201 ggtcccttcg aatgtgacct tgtcgaagat gccgcccgac agatcgcacc tctctagagc
     4261 cagtgcgccg atcgagcggt tgctgatcgc ttcgccgttg cgcacggcct gctgcaattg
     4321 ttcacgtgcg gacatcatgc gcttgccctg cgtaacggat gaaacacggt ctgctcgagg
     4381 taggcatggt caaggcgcgt ggatgcatcg atcgatgctt cggcaagatt ggcgcggaac
     4441 agattcgctt cgtcgaagtc ggcgtcgtgc agcatggcgc ggcgcatttg cgcgccgatc
     4501 aggtcggcgc cggcgaggct ggcgccgctc aggtcggtgc gaaccagata gccaccgtcg
     4561 aacttcgcat cgctcaggtc ggcttgctgc agaccggcat ccgaaaagtc gcattggctg
     4621 atcgttgcgc cgccgaatcg cgcgttgcgg gcctgtgtat gccgcagatt cacttcggtc
     4681 agcgacgcct gggagaagtc cgcacgatcc agtacggtgt ctttcgcgaa gcacgctttg
     4741 tgaagccgcg cgtggttgaa acggatctgc tgcgcaacgg tttcgacaaa gccaaaaccc
     4801 tcgatgttgg cggacgtgaa agcaacgcgg tcgagccggc actgaatgaa cgcgagcttg
     4861 cggatcgtcg ccgcgtggag ggtcacgtcg gtcagtgtct gctcgatgaa gaccaactcg
     4921 ctgagcgtgg cttccgcgaa atcgaccgct tcgaaacggc agccgctgaa ctggactcgt
     4981 cttgccgatg cacgccgcgc ggtgcagttt cgccagaccg ctcgttcgaa cagacagcgg
     5041 tcgaggaccg cttcgctgaa gtcggttccc tcgcagtcgg cgagcgaaag gttggcgcct
     5101 gagagatcgg ccctggcgaa tgtggtcccg gcgagcgtgg cgcgcgcgat gaccgcgtcg
     5161 cgcaagttgg cgcctgtcag gtcggtgccg ctcagatcgg cgctttcgag cagcgcaccg
     5221 gcaaggtcca cgtcgcgcaa attcatccca ctcagatcgg cgccggtgag gtcgaggccg
     5281 gcgagcgatt cgccgcggcc atggccggcg gcaacgcgtt cgcgcagtgc cgcggaagac
     5341 gcagtgtcga ggcgtgccgc ggcttcctga tagtgggccg actgcaaata ggatgcgcgc
     5401 agtttgcgct cggcttcccg ctcctgttcg agcatttcgg gccgtgcagg gtgctgggcg
     5461 cgattgcttg cgaaagactg gggaggtcca cgtcttgccg cagcgcttcg gcttgcggtg
     5521 agttcggggt gctcggcctc gatccgctga cgcgtctgtt cgagcgcggc caactgagtc
     5581 tcggcgagct tttcattgcg ctcgacgaac tcggccagtt cgtcgagacg cggtgggcgt
     5641 gtggctagca cttctggcgc agagatgacg accccggcag cgcccgcctg gctagcctgt
     5701 tcgcgggcgc gttgttgcag attgcgttgc aatgcgctgc cttgtggtcc ggcttgcgcg
     5761 tcaccgtcgc cgtccgcatc ctccgcggcg gctagcattg attgcggcgc gaggtccagg
     5821 tcgcgcaggg cgtgcaacac gccgcgcttg ctgtccatgc gcagatcgaa cacgcgccga
     5881 tagtgggcgt cgctgcgcgg ttcgttatgg cattcgacgg cgagcagcaa acacgccacg
     5941 tcctgcgcgt cgaattcctg gacccgcgcc gcgccatgaa aaatcatgat ggcccgttcg
     6001 cggtgtggaa tgaaccatac cgtcgtcagc cgcatgccga cttcggtcaa gtccgattca
     6061 cgtgtccgct gcacgaagca acgcgcgtgc aggggcggca acgcgccggc cagtgtcggg
     6121 cgctccggat gcatgtgggt caattcatac gctgcacccg gcggcaggga atcgaggtga
     6181 gggaattgct gatcccgcgg cgcgacgttg aaataatccg catgcatcgt gcgcggaaag
     6241 cctgggcaat cctccttgag ccaccgctcg tccatctcgc catatagcgc gctgcgctgc
     6301 ggccagtcag cggcgatcgg cgtgaaggcc gccggtgcga tgccgcgttc gcgcgtgacg
     6361 actggcgcgt cctgatacgc aatgtgcgga agcaactgcg cggtgcccgg cgcgccggtg
     6421 ggaaaaccga tgccacgtgg attgtcggca tagcctgggc cgccgtaggc cagtggccaa
     6481 tcgagcgcca ggcattcgaa cggcgtgggt tgcgacggcg tgtcgccgtt ccactcacgc
     6541 gcgcccgaca cctgcaattg cttgtcgagg ccggcgaatc gaatgccgac acggcacatg
     6601 tgagccgcat tgcaatactg gccgtaggcg cgccccgcga ccagaaactc ggcatgcagt
     6661 ttcggcagcg cggcgtcgag tgcatagccg cccaactcgc tgccaaccgt attccacagt
     6721 tccgattcgc cgacgaggcg gggtgcgttg ccgagcgtca ccatcagcgg tatcgcgatg
     6781 ccgaggtgct cgttgccggc ctgccgatac gtacgggtca gaatgccgac gggggtaggt
     6841 ttgatgattt tcatcggata tcaggcgcgc agcggtttca gaaagtcacc gagaacccga
     6901 cggtcgagaa cgacacgccg gtcgcactga cgctcgatcc cgtgctgctg accgacgacc
     6961 cggttgtaga caccgaactg ccggttgcgc ccacgtccga tccggtcgcg ctgacgctgg
     7021 atccggttgc accgacgctt gagccggtcg ccgtgacgat cgagccggtc acgaagagct
     7081 gtgccgtggt gatgcccgtt tgcaagccga tcacgatcga ggtcgagctg ccgctgaccg
     7141 tgacggccga gttgccgccc acggtgctgg tggagttggc ggccacgctg gatgtcgaat
     7201 cggcgccaac actggtggtg gagttactgg cgacgctcag gctgtggtcg ctgcccacgg
     7261 tatgcctcat gtcaccggcg accgtcagcg tgtgcttacg atccaccgta tgggtcgaat
     7321 cgtttgagac attcaggctg tagttgcggc cgatggtgtg cgtttcatcg ttggccacct
     7381 cggcgctgga gttgttgccg accttgcggc tctggttgct ggtgatcgac gcggcatagt
     7441 cgttaccgat cgataccgtg tagtcgcggc ccacgccgcg cgtcgcatcg ttgctcacat
     7501 tgacggcttc gttgcggccg acctggcgcg tcgcgtcgtt ctcgacggag acgtcgtaat
     7561 tgcgctccgc atggagggca atctgctccg cgcccgcttt gtcttcgaaa cgaagcatgt
     7621 tggcgttagc gctgctgccg cctggcgtgc gcgtgacgaa gccgctttgc gtggcggctt
     7681 ccggcagcgc gaacggcggc atctgatcgg cgttgtagat gcgtccggtg atgaccggac
     7741 ggtcaagcgc gccgttgaga aaatcgacca ccacctcctg gccgacacgc ggaatatgaa
     7801 tgctgccgaa gcccgagccg gcccacgcgt cggacacgcg aatccagcac gagctgttct
     7861 cgtcgcgttt gccgtaacgg tcccagcgga attgcacttt cacgcggccg tattgatcgg
     7921 tccagatgtc ctggcctgcc ggtccgacga ccaccgcggt ttgtgggccg gtggctttgg
     7981 gcaccggcgt cacgctgggc gcgcgaaagg gcagcgaagc aggttgcgca acgagatcga
     8041 agtcatattg cgcgtggctg ctgccgcttg catagccgcc ttcctgcagc cggtagacca
     8101 cgctcaccag caggtattcg cgattctccg cttgccgggg acagtcctgc agcgtgaagc
     8161 gatagcccgg cgcggcctca cgcaaacggg tatgggcgcg tacgcgcgcg ttcgccgcct
     8221 gttcttcctc gagacgcatg cgcacgtaat gctcgccctg ctcggcttcg atgtagccgc
     8281 cttgccatgc atacctggca tagtcgccgt gctcggcgct cgaagactgg gcccgcattt
     8341 gcgacagatc ggcgcgcggt ttgcggaagt cgtagtcgtc gacggcgaac gcgcccgcgc
     8401 tgatctgggt ggcgggacgc cactcatcaa taccctcttc gcgcggcagc gcaaggcggt
     8461 cgggcgactg atacggcagc atggcgtgtt cgggcaacgg gtcgtgcgcg ctggcgtcgt
     8521 cggcgagcac caggacctgc tgaccggctt cgtggcggaa gtagtaatag atgccttcct
     8581 gttccatcag ccggctgacg aagttgaaat cgctttcctg gtactgcacg caatagtccc
     8641 agcttcgata gcttgcgagc aggcgttctt caacgggaaa gccatatttg ccgatgacct
     8701 cgcgcagaat ctcgaccgcc gtgtggttct gaaaaatgcg gcagtcggtt gttttggtca
     8761 ggtaccagag ccatggccgc agcgtgaccg catagatgta ggtgcgcggc gtatcggtct
     8821 cgcgtccggc gaattcgcag cgcacgatct ggccgccgag gtatcgggtg ctgccgttga
     8881 gcgtgacgat gtggagcgtc agcggcttgc caagcagcgc gttcatatcg agcgaagcgg
     8941 acggactcac cagctccacg ttgaattcat acagcgccga cagagactcc tcgccgcgta
     9001 gcgagcggaa tcgcagcatc tgcggatcga gtggcgtgtg cgcgatgatg atgtggttca
     9061 tggcagcttc tttaaagacc cgactgagcg ctggccgcgc gggtgttgcg ggtgtcgcga
     9121 gtttcaggtt gataaacgag atcgtcggca gtgagcgtgc tggttcgcgt gccgagccaa
     9181 ctggcgcagc ccagcggccg gccggcgcca agtgcgatgc cggcaacctg atcggcggcc
     9241 atcagcaggc gcacgtccca cgaccactcg acaccggcgt attgccgcac caaatccgtc
     9301 aactgctgcg aaagcggcgt gccgggtagc aatgccgcgt attgctcgcg tgacaagggg
     9361 ccgagccgaa cctcgaattt cgtctgcgcg tcgcgaaccg ccgcacccag catggcgtcg
     9421 gcgccaagcc ggcgggtgtt cgcgtgcgtg cgtaacgcgg tgcgccgcgg cgcctcgacc
     9481 ggcagccatt gcggcagcca ttcgacgatc tggacgggta cgccgaggca ggcgcgcagg
     9541 atgctccgca gaccctccgc gttgcgcgtc tgtcgcacca ggtggccggc catgaaatat
     9601 ctggcgtgat ccgacagcgt gtcgggtcgc cgcgcctcgc gccgcggcag atggatgagg
     9661 ctcgccaggt agcgatcgaa ccgtccgccc tcgccacggt cgaggctgac cgtaggctgc
     9721 gcatcggccc aggcgcgata gaacagcagg atcagccgat gatgaaacag atcggcgaat
     9781 gcgctcagcg cgtggtcgtc gtgatggcga gcacgctcgc gcacgaactc ggtcagatgc
     9841 agcggcagag gaccgttcgg gccgaataga ccaaagctgt gaatcaggac acgcggcttc
     9901 gcgccttctg gcacagacgc ggcggcgagc gttgccggcg cgaaggcgag cgacggtagc
     9961 tggccgatcc gcaacggttc atcggcggga tgcgccgcgc gtcccaacgg cgccggggcc
    10021 gccgcgcgcg cgtccagcca gcgcaacagg ttgtacagat cgtacttgta tggcgcatcg
    10081 gcaacgcatt gccagaaatg cgcgagcata gaactggcgt gcggcacacg cgacgacggg
    10141 gcgattgcgt tgctcatgct gtggggcggc ttccgacgcg aggtggccaa cgtttcacta
    10201 gtccacgttg cagcgtggct accgcgagtt cgctaaagct attcaatgac acgtggcgcg
    10261 caaaaaattg ctccagcacc gcgccgaata aatacgtgct cgcgcccgca aatgaggttt
    10321 cgtcgagcag cagcgacacc gccacaccgc ggccgaacat cggcggtccc gcctgaggca
    10381 actgacggca gaccggctca acccgaacgc tgcgtacgcc gtcaatttgc ggccgcacag
    10441 ctgcgtcggc tgcgctcgca tagagttcga gcaattcgcg caaggcctgc gccccctgca
    10501 cactgtcaag atcgctcagc gtgaggtagt cgagcccgag atgcttgatg agccgccatg
    10561 tgatttcgtt ttccgcaagg ggcggctgcg gcggcgtggg ccgtcggagc gcctggatgg
    10621 cggcgaccgg ggccgaggtg cgcagactga agtcgctagt gctgccggtt ggcatcagca
    10681 gcgcgagatc gcggttggta cacatggcgt cgacggacag gtgccgcaag tcgtcggaaa
    10741 acggcgcttc gtgctgatcc accaacgata tgaatacttc gctgccggca taggcagatc
    10801 tcgtgctgct gcgcgctgcg cggcttgcag catggtcgcg tggggcgacg agccgcgctt
    10861 cgcgccgcat cgaaaaatag cggccgcgat cggcgtggtc gtgagagagg ccgccgtaga
    10921 aaggatgaaa ctcacagtgc ccgccgtcag actggcgatg gcccaccact ttctcgacgc
    10981 cgtaaatttc gtagtccagc ggccgcgtgc gatcgactac gacatgatgc tcataccgct
    11041 gattcgtgat ggcgatgcga tcggtgcgct tcgggaacag gttgatcgca ggcgtgcaaa
    11101 acaacgcgag gctgcgcaga tcgacggacc gttcgagctc gggcgacgct tcgtcgagca
    11161 gaacggtcag cgagaacgtg gtgccgtcga tgttttgcag cgcgcgttgc agcgaaccga
    11221 tgctgaaaaa cataaaacgt tggggaaagg cgaaatactc gtgcagcagc cggtatccct
    11281 ggaacgaacg cgaaccgtat ggcagcaacg cctgggcagc atcgaaacct tcctggcgga
    11341 tggcctcgcc gtcgagcagc gagcacgcgc tgccagtgtg gcttgtgttg cccgagaccg
    11401 aaggcccgtg gcagatgacg ccgagttgat gacctagcac ctgttccagc agcggcagcg
    11461 cctgattgtc cggaccgctg agaaaaaacg tcaacgaatc gagcggcaac tcgcgcagat
    11521 tgccgacgcc gctggcttcc agttgaatat gcagcgcgct gcgcacgcgg cttgtcagct
    11581 ggccatgcgg caatggcaga tcggcgggga cgccgcccag tttgacttcg gtgatacgca
    11641 gcggccacaa cgtgacatcg tgcgcggtgc tgaagtcaca ggcggtctgt tcgcctggcg
    11701 ccagattgcc gcgcagcgac gtgccggccg gcagcgtata gccggacagc agactgcctt
    11761 cgcgcaggtt cggttgcacg gcgacgatcg tcatcgacgg cagcggagcg aggtaattcg
    11821 gatagaccac gtcgagcagg cgctgggaga aacgcggaaa ctcggcgtcc atttttaact
    11881 ggatgcgcgc ggtcaggaag ctgaaacctt caaggagccg ctcgacatac ggatcgctca
    11941 cctcgatccc gcgcatgccc agttgtccgg cggcctgcgg gaattcggcc gcgaattcgg
    12001 cgccgagctc gcgcagatac gcaagttcac ggttgtaata gtcgagcagg cgggcgtcca
    12061 ttacgcggct tcctgcgaac ggaacaccac atggccgct
//
LOCUS       DATASH010000711         1947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6443545,
            whole genome shotgun sequence.
ACCESSION   DATASH010000711 DATASH010000000
VERSION     DATASH010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6443545"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VHV99_18885"
     CDS             complement(<1..243)
                     /locus_tag="VHV99_18885"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012399600.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX3637512.1"
                     /translation="MTDFVTDPVVIVSAVRTPMAAFQGDFATVTAPQLGSIAIRAAVE
                     RAGLKPEQIEEAVMGCVLPAGLGQAPARQAALGAGLP"
     gene            complement(236..1030)
                     /gene="can"
                     /locus_tag="VHV99_18890"
     CDS             complement(236..1030)
                     /gene="can"
                     /locus_tag="VHV99_18890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006999972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HEX3637513.1"
                     /translation="MPNIDNTDAPESQDNNPLSHLFDNNLKWVDRKLSEDAEYFSRLA
                     HQQAPEYLWIGCSDSRVPANQIIGLPPGEVFVHRNIANVVVHSDLNCLSVIQFAVDLL
                     KVKHIMVVGHYGCSGVGAALVSRRVGLADNWLRHVEDVRDKHAALLGEWPMGEARNRR
                     LVELNTIEQTVNVCHTTILRDAWARGQEITVHGWTYGVHDGRVRNMGMMINGSEEVEP
                     TYQQCIAALARGGAHSSENDVLASDAARLGDVPAIVKGVIKELKHD"
     gene            complement(1052..>1947)
                     /gene="aceK"
                     /locus_tag="VHV99_18895"
     CDS             complement(1052..>1947)
                     /gene="aceK"
                     /locus_tag="VHV99_18895"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016344023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HEX3637514.1"
                     /translation="KQGKNLFYRDLLRHLSHSSDQFIIAPGIKGLVMLVFTLPSFPYV
                     FKLIKDAFPPPKETTRKKIQDKYQLVKRHDRLGRMADTLEYSSVALPLSRLDDALLKE
                     LTREVPSMIEYEGDNLVIRHLYIERRMTPLNLYLQNGTLSEIEHGIKEYGDAIKELIQ
                     ANIFPGDMLYKNFGVTRHGRVVFYDYDEIEYLTDCNVRRVPQARNEEDEMSGEPWYTV
                     GPHDIFPETYGTFLLGDPRVRNVFMKHHADFFDPALWQKHKEQILNGELAEFFPYERS
                     IRFCVRYPERFLSGAPAARAA"
BASE COUNT          368 a          585 c          593 g          401 t
ORIGIN      
        1 cggcaagccc gcgccaagcg cggcttgccg cgcgggcgcc tggccgagac cggcgggcag
       61 cacgcaaccc atgacggctt cttcgatctg ctcgggcttg agtccggcgc gctccacggc
      121 ggcgcgaatc gcgatcgaac cgagttgcgg cgcggtcacc gtggcgaagt cgccctggaa
      181 agcggccatt ggcgtgcgga cagccgatac gatcaccacc ggatcagtca cgaaatcagt
      241 catgtttcag ctccttgatc acacctttca caatagccgg gacatcgccg agacgggcgg
      301 catcagaggc gagcacatcg ttctccgatg aatgcgcgcc accgcgcgcc agcgccgcga
      361 tgcattgctg ataggtcggc tcgacctctt ccgacccgtt gatcatcatg cccatgttgc
      421 gcacacggcc gtcatgcacg ccataggtcc agccgtgcac cgtgatctcc tgacctcgcg
      481 cccacgcatc gcgcaggatg gtcgtgtggc atacgttcac cgtttgttca atcgtgttca
      541 gctccaccag ccgacgattg cgcgcctcgc ccatcggcca ttccccgagc agcgcggcgt
      601 gcttgtcgcg cacgtcttcc acgtgacgca gccagttatc ggccaggcct acgcgccggc
      661 tcacgagcgc cgcgcctacg ccggaacagc cgtagtgccc gaccaccatg atgtgcttga
      721 ccttcagcag gtcgaccgca aactggatca cagacaagca gttgagatcc gagtgaacga
      781 ccacgttggc gatgttccta tgtacgaaca cctcgcccgg cggcaagcca atgatctggt
      841 tcgccggcac tcgcgagtcc gagcagccga tccacaagta ttcgggcgct tgctggtgcg
      901 cgaggcgaga gaaatattca gcgtcttccg aaagcttgcg atcgacccac ttcaggttgt
      961 tatcgaacag gtgcgagagc gggttgttgt cctggctttc aggtgcgtcg gtgttgtcga
     1021 tattgggcat ggcattcagt gcggataggt ttcatgcggc gcgtgctgcg ggtgcgccgg
     1081 acagaaaacg ctcaggataa cgaacgcaaa aacggatgct gcgttcgtac gggaagaact
     1141 cggcgagctc gccgttaagg atctgttcct tgtgcttttg ccagagagcg gggtcgaaga
     1201 aatcggcgtg atgtttcatg aacacgttgc gcacgcgcgg gtcgccgagc aggaaggtgc
     1261 cgtaggtttc ggggaagatg tcatgcggcc cgacggtgta ccacggctcg cccgacatct
     1321 cgtcttcttc gttgcgcgct tgcggcaccc ggcgcacgtt gcagtcggtc aggtattcga
     1381 tctcgtcata gtcgtagaac accacgcggc cgtgacgcgt gacgccgaaa ttcttgtaga
     1441 gcatgtcgcc ggggaaaatg ttcgcctgga tcagttcctt gatcgcgtcg ccatattcct
     1501 tgattccatg ttcgatttcc gacagcgtgc cgttctgcaa atacaggttc agcggcgtca
     1561 tgcggcgctc gatgtagaga tgcctgatca cgaggttatc gccttcatat tcgatcatcg
     1621 aaggcacttc cctggtgagc tccttgagca gcgcatcgtc gaggcgcgac agcggtagcg
     1681 cgacgctcga atattcgagc gtatcggcca tgcgtccaag gcggtcatgg cgtttgacga
     1741 gctgatactt gtcctggatc ttcttgcgcg tggtttcctt cggcggcggg aaggcgtcct
     1801 tgatcagctt gaacacgtac ggaaacgacg gcagcgtgaa caccagcatc acgagcccct
     1861 tgatgccggg cgcgatgatg aactggtcgc tcgaatgcga caggtgacgc agcagatcgc
     1921 gatagaacag attcttgccc tgctttt
//
LOCUS       DATASH010000712         2597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_115708,
            whole genome shotgun sequence.
ACCESSION   DATASH010000712 DATASH010000000
VERSION     DATASH010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115708"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(234..2219)
                     /locus_tag="VHV99_18900"
     CDS             complement(234..2219)
                     /locus_tag="VHV99_18900"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019254672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEX3637515.1"
                     /translation="MSTKKPNAAYSEASIKVLKGLEPVKQRPGMYTRTENPLHIIQEV
                     IDNASDEALGGHGRQITVTLHADHSVSVEDDGRGIPFGLHPEEGVPVVEIVFTRLHAG
                     GKFDKAAGGAYTFSGGLHGVGVSVTNALSTRLDVTVWRDGKVAELSFANGDVAKQLEV
                     RPATKADKKSGTRVTAWANPKYFDSPNLPFGELQRLLRSKAVLLPGVEDTLVNEKTGE
                     QQTWKYEDGLRGYLLEGMNGSDLLIPLFEGERYVENSRASEETFAEGEGAAWVVAWSE
                     EGTLMRESYVNLIPTPAGGTHESGLRDGLFQAVKNFVELHNLQPKGVKLLAEDVFARV
                     SFVLSAKVLDPQFQGQIKERLNSRDAVKLVSSFSRPALELWLNQHVEHGKKLADLVIK
                     QAQARTRAGQKVEKRKSSGVAVLPGKLTDCESTEIGRNELFLVEGDSAGGSAKMGRDK
                     EYQAILPLRGKVLNTWETERDRLFANNEVHDISVAIGVDPHSPDDKVDLSNLRYGKIC
                     ILSDADVDGSHIQVLLLTLFFKHFPQLIERGHVCVARPPLFRVDAPARGKKPAQKLYA
                     LDEGELEAILDKLRKDGVRDTQWSISRFKGLGEMSAEQLWDTTMNPDTRRLTPVALGE
                     LDYDATVARMTMLMGKGEAASRRSWLEEKGNEVEADI"
BASE COUNT          441 a          825 c          792 g          539 t
ORIGIN      
        1 cgctgcaccg gcttctggcc gtcgcagacg tccggcagcg cgcggccctt gaccacgctt
       61 accgcgtagt cgaggtactg gcgttccgca tagttgccga gcgtcaggaa gtcgccttcc
      121 gggggcggcg gctcggtgaa aaggtcgaga gtgttatcgt cgtccatcta gattccgtat
      181 ccgtgtttgg cgtgaagttt tcgtgcgaag gtggtgcgct atctcacgcg cgctcagata
      241 tccgcttcca cttcgttgcc cttttcttcc agccagctgc ggcgcgacgc cgcttcaccc
      301 ttgcccatca gcatggtcat gcgcgccacc gtggcgtcgt agtcgagttc gccgagcgcc
      361 accggcgtca ggcgccgcgt gtcggggttc atcgtggtgt cccacaattg ctccgcgctc
      421 atttcgccca ggcctttgaa gcggctgata gaccactgcg tgtcgcgcac accgtccttg
      481 cgcagcttgt cgaggatcgc ttcgagttcg ccttcgtcga gcgcgtagag cttctgcgcc
      541 ggcttcttgc cgcgcgcggg cgcatcgacc cggaacagcg gcggacgcgc cacgcacaca
      601 tgcccgcgtt cgatcagctg cgggaaatgc ttgaagaaca gcgtgagcag cagcacctgg
      661 atgtgcgaac cgtcgacatc tgcatccgac aagatgcaga tcttgccgta acgcagattc
      721 gacagatcga ccttgtcgtc cgggctatgc ggatcgacgc cgatcgccac cgaaatgtcg
      781 tgcacttcgt tgttggcgaa caggcggtcg cgctcggttt cccacgtgtt cagcaccttg
      841 ccgcgcagcg gcaggatcgc ctggtattcc ttgtcgcggc ccatcttcgc cgagccgccc
      901 gccgaatcgc cctcgacgag gaacagttcg ttgcggccga tttccgtcga ttcgcaatcg
      961 gtcagcttgc ccggcaacac ggccacgccg gagctcttgc gcttctcgac cttctggccg
     1021 gcacgcgtac gcgcctgcgc ctgtttgata acgaggtcgg cgagtttctt gccgtgctcg
     1081 acgtgctggt tcagccacag ctccaaagcc ggccgcgaga acgacgaaac cagcttgacc
     1141 gcatcacggc tattcaggcg ctccttgatc tgcccttgga attgcggatc gagcaccttc
     1201 gccgacagca cgaacgacac gcgcgcgaac acgtcttccg ccagcagctt cacgcccttc
     1261 ggctgcaggt tatgcaactc gacaaagttc ttgaccgcct ggaacagacc gtcacgcaaa
     1321 ccggattcgt gcgtaccgcc cgcgggcgtc ggaatcaggt tgacgtacga ctcgcgcatg
     1381 agcgtccctt cttcgctcca cgcgacgacc catgcggcgc cttcgccttc agcaaacgtc
     1441 tcttcgctgg cgcgcgagtt ttcgacatag cgctcgcctt cgaacagcgg aatcagcaga
     1501 tcgctgccgt tcatgccttc gagcagatag ccgcgcaaac cgtcttcgta tttccaggtt
     1561 tgctgctcgc cggttttctc gttgaccagc gtgtcttcga cgcccggcag cagcaccgct
     1621 ttcgagcgca gcagacgctg cagttcgccg aacggcaggt tcggcgaatc gaaatatttc
     1681 ggattggccc acgcagtgac gcgcgtgccg gacttcttgt cggctttggt tgcggggcga
     1741 acttcaagct gtttcgcgac gtcgccattg gcaaaactca gctcggcgac cttgccgtcg
     1801 cgccacacgg tgacgtcgag gcgcgtggac agcgcgttag tgaccgacac gccgacgccg
     1861 tgcaagccgc cagagaacgt gtacgcgccg ccagcggctt tatcgaactt gccgccagca
     1921 tgcaggcgcg tgaaaacgat ctcgacgacc ggtacgcctt cttccggatg caggccgaac
     1981 gggatgccgc ggccgtcgtc ttcgaccgag accgaatggt ccgcgtgcag cgtgaccgtg
     2041 atttgccggc catggccgcc gagggcctcg tccgacgcgt tgtcgataac ttcctgaatg
     2101 atgtgcagcg gattctcggt gcgggtgtac atcccgggcc gttgcttgac cggctccaga
     2161 cccttcaaca ccttgatcga cgcttcgcta tacgcggcgt ttggcttttt cgtagacatg
     2221 gttgactcgg gtgcgtcggt tcatcgttgt ccacaggtcc tgtggacagg tccgtggaca
     2281 atgcgttgag ttttcaaaaa aagcgaaacg aaacaacagg gttaggtggg atgcccgcag
     2341 tgcgggcaag ctgttttgtc gcccggcttc ccgtaggcgc gctcgacggc ggctcgatgc
     2401 cggttctaca gcccggtaac ggccagccgg tgcgaacgcc ggaggaagcc tgtccgcggg
     2461 gtattttact gatttcggta cgggcggcaa ttcacggcat gcgggattgg ccgtttggat
     2521 caggggcagc gtacggcttc ggccaattat gcgaccgcga tgagaccgct agaggagcgt
     2581 gaaaacatga cgacggg
//
LOCUS       DATASH010000713         3714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_907896,
            whole genome shotgun sequence.
ACCESSION   DATASH010000713 DATASH010000000
VERSION     DATASH010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_907896"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VHV99_18905"
     CDS             <1..240
                     /locus_tag="VHV99_18905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HEX3637516.1"
                     /translation="ATGKILQVTYWNTANGQSFALVPVKGRQLLFVNTISASGAKYQA
                     GSYTWWTKGPRADLYDATDGENAPPMLSDCVTINR"
     gene            509..2161
                     /locus_tag="VHV99_18910"
     CDS             509..2161
                     /locus_tag="VHV99_18910"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009978184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEX3637517.1"
                     /translation="MKASDLFVKSLEAEGVEYVFGIPGEENLDLLESLRRSKIRLILT
                     RHEQAAGFMAATYGRLTGRTGVCLSTLGPGATNFVTAAAYAQLGGMPMLMVTGQKPIK
                     SSKQGHFQIVDVVRMMEPLTKYTRQIVSIANIPASVREAVRQAEEERPGAVHLELPED
                     VADEEGDGKPIPKSYSRRPVAEEKAVARAVEAITKAKHPLLMIGAGGNRKTTTRMLRE
                     FVDQIGIPFFTTQMGKGVIDESHPMWLGNATLSDGDFVHRAIDHADCIINVGHDVIEK
                     PPFFMRSGDTGEKTVIHVNFLGAEVDTVYFPQIEVVGDIANAVWQLKESLKERQEHWD
                     FTRFKEIKQHFEEHLVKGQHDDRFPMYPVRIVNDVYETTPVDGIVCLDNGMYKIWFAR
                     YYRAHEPNSLLLDNALASMGAGLPSAIATKIVHPDRKVMAVCGDGGFMMNSQELETAV
                     RLKLDLVILILRDDAFGMIRWKQENMNFPDYGMTLSNPDFVAYAQSYGAKGHRIESAA
                     EFAPLLRECFATPGVHVIDLPIDYSDNERVLNREIKRLSAQL"
     gene            2272..3708
                     /locus_tag="VHV99_18915"
     CDS             2272..3708
                     /locus_tag="VHV99_18915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX3637518.1"
                     /translation="MLNKTYPYYLANVAVAANTDLEVTDKFSGEVATRVAMADATAID
                     KAIGYAVDAMPALRAFPPFKRQAVLEHCVKRFRERYDELALALCIEAGKPINDSKGEV
                     TRLIDTFKVAAEESVRIDGEIVNLEISPRAKGYHGYVKRVPIGPCSFISPFNFPLNLT
                     AHKVAPAIAAGVPFVLKPASRTPVGALIMGEILAETDLPKGAFSILPAHRDGADLFTT
                     DERFKLLSFTGSPAVGWELKKKAGKKKVILELGGNAAAIVDGDQGEKLDYVVDRLAFG
                     AFYQSGQSCIGVQRILVHAKVYDALREKLIAKTKSLVMGDPKDEKTFVGPMISESESR
                     RLAGWMESAVQAGAKIVAGGKVDGAMFEATLLEGVKRDTDLYRKEAFGPVAILERFDD
                     FDAALATVNDSDFGLQAGVFTDSLAHAHRAWDVLEVGGVVINDVPSFRVDNMPYGGVK
                     DSGLGREGVRYAIEDMTEMRLMVMRETW"
BASE COUNT          738 a         1080 c         1197 g          699 t
ORIGIN      
        1 gcgaccggca agattctgca agtgacctac tggaacaccg cgaacggcca gagcttcgcg
       61 ctcgtgcccg taaaaggccg gcaactgctg ttcgtcaaca cgatatcggc ttcaggcgcg
      121 aagtatcagg ccggcagcta cacctggtgg accaagggcc cgcgcgccga cctgtacgac
      181 gcgaccgacg gtgaaaacgc cccgccgatg ctgtcggact gcgtcaccat caatcgctaa
      241 gcatgccgcg gcggttttcc tcaactacgc cgcctgcctc actttgcctc cgccttgcgg
      301 cgctgcccgc ctgcgcacat ccttttcgtt cgagtagtaa gctgtttggc atggtatccg
      361 ctgtgtccgc cgttactgct gtccccgctg tcaccgttct gtcgggccgc gcgcggttcg
      421 cgtggctgtc gtcgtgttcg atcgtccgtt ttccgacctc aacggatggg cccatggccc
      481 gcgtccgttt catcaagcga gaccctccat gaaagcatcg gacctgttcg tcaaatcgct
      541 ggaagccgaa ggtgtcgagt atgtgttcgg catccccggt gaagaaaacc tcgatctcct
      601 cgaatcgctg cgccgctcga aaattcgcct gattctcacg cgtcacgaac aggcagcggg
      661 tttcatggcc gccacctatg gacgcctcac cgggcgcacc ggtgtgtgtc tgtccaccct
      721 cgggccgggc gcgaccaact tcgtgaccgc cgcagcatac gcgcagttag gcggcatgcc
      781 gatgctgatg gtgacgggac aaaagccgat caagtcgagc aagcaggggc atttccagat
      841 cgtggacgta gtgcggatga tggagccgct caccaagtac acgcggcaga tcgtgtcgat
      901 cgccaacatt ccggcctcgg tgcgcgaggc ggtgcggcag gccgaagaag agcggcccgg
      961 cgccgtgcac ctcgaattgc ccgaggacgt cgcggatgaa gagggcgacg gcaagccgat
     1021 tccgaagagc tatagccgcc gtcccgtcgc cgaagaaaaa gcggtggcgc gcgcggtgga
     1081 ggcaatcacg aaagcgaagc atccgctgtt gatgatcggc gcgggcggca accgcaagac
     1141 caccaccagg atgctgcgcg aattcgtcga ccagatcggc attccgtttt tcacgacgca
     1201 gatgggcaag ggcgtgatcg acgaatcgca tcccatgtgg ctcggcaacg ccaccttgtc
     1261 cgacggcgac ttcgtgcatc gcgcgatcga tcacgcggac tgcatcatca atgtcggcca
     1321 cgatgtgatc gagaagccgc cgttcttcat gcgcagcggc gacactggcg aaaagacggt
     1381 gattcacgtc aactttctcg gcgcggaagt ggatacggtg tatttcccgc agatcgaagt
     1441 ggtcggcgat attgccaacg cagtctggca gctcaaggaa agtctcaagg agcggcagga
     1501 acattgggac ttcacgcgct tcaaggaaat caagcagcac ttcgaagagc atctggtcaa
     1561 aggccagcac gacgaccgtt tcccgatgta cccggtgcgg atcgtcaacg acgtctacga
     1621 gaccacgccg gtggacggca tcgtgtgtct ggacaacggt atgtacaaga tctggtttgc
     1681 gcgctattac cgtgcgcacg aaccgaattc gctgctgctc gataacgcct tggcgtcgat
     1741 gggcgccggc ttgccgtcgg cgatcgcgac caaaatcgtg cacccggacc gcaaggtcat
     1801 ggctgtctgc ggcgacggcg gcttcatgat gaattcgcag gaactggaaa cggcggtgcg
     1861 tctgaagctc gatctggtga tcctgattct gcgcgacgac gcgttcggca tgatccgctg
     1921 gaagcaggaa aacatgaact tcccggacta cggcatgacg ctgagcaatc cggatttcgt
     1981 cgcgtatgcg cagagttacg gcgcaaaagg tcatcgcatt gaatcggctg cggagttcgc
     2041 gccgttgctg cgcgaatgtt tcgcgacgcc cggcgtgcat gtgatcgatt tgccgatcga
     2101 ctactcggac aacgagcgcg tgctgaatcg cgagatcaag cggctctcag cacaactatg
     2161 agcgcgtaac ggtgaacgcg taacgacgaa cggaacagca atgagcggca gcggggcata
     2221 gcccttgctg gccgtatgac tcatgtcggt tgtcggaaag gagaacgtgt catgttgaac
     2281 aagacttacc cgtactacct cgcaaacgtt gcggtggccg ccaacaccga tctcgaagtt
     2341 accgataaat tcagcggcga agtggcgacc cgcgtggcga tggcagacgc gacggcgatc
     2401 gacaaagcga tcggttacgc ggtcgacgcc atgcccgctt tgcgcgcgtt tccgcccttc
     2461 aagcgtcaag ccgtgctcga acattgcgtg aagcgctttc gcgagcgcta cgatgaattg
     2521 gcgctcgcgc tttgtatcga agccggcaag ccgatcaacg actcgaaagg cgaggtcacg
     2581 cgtctgatcg atacgttcaa ggtggcggct gaagagtcgg tgcgcatcga tggcgaaatc
     2641 gtcaatctgg agatttcgcc gcgcgcgaag ggctatcacg gctacgtgaa gcgcgtgccg
     2701 atcgggccgt gctcgtttat ctcgccgttc aattttccgc tgaacctgac cgcgcacaaa
     2761 gtcgcgccgg cgatcgccgc cggcgtgccg ttcgtgttga agcccgcgag ccgcacgccg
     2821 gtcggcgcac tgatcatggg cgagattctg gcggaaaccg atttgccgaa aggcgcgttc
     2881 tcgattctgc cggcacatcg cgacggcgcc gacctgttca ccaccgatga gcgtttcaag
     2941 ctgctgtcct ttaccggctc gcccgcagtg ggctgggagc tgaagaaaaa ggccggcaag
     3001 aagaaggtga ttctggagtt gggcggcaac gcggccgcga tcgtcgacgg cgatcagggt
     3061 gagaagctcg actacgtggt cgaccggctg gcgttcggcg cgttctatca gtcggggcag
     3121 agttgcatcg gcgtgcagcg gattctggtg catgcgaagg tctacgacgc gctgcgcgag
     3181 aaactgatcg ccaaaacgaa gtcgctggtg atgggcgatc cgaaggacga aaagaccttc
     3241 gttggtccga tgatctccga atccgagtcc aggcgtctgg ccggctggat ggaaagcgcg
     3301 gtgcaggcgg gcgcgaagat cgtcgcgggc ggcaaggtcg acggcgcgat gttcgaagcc
     3361 accttgctcg aaggcgtaaa gcgcgacacc gatctgtatc gtaaggaagc gttcgggccg
     3421 gtcgcgattc tggagcgttt cgacgatttc gacgccgcgc tggccaccgt caacgatagc
     3481 gacttcggcc tgcaggcggg cgtgttcacc gattcgctcg ctcatgcgca tcgcgcgtgg
     3541 gatgtgctcg aagtgggcgg cgtggtgatc aatgacgtgc cgtcgttccg ggtcgacaac
     3601 atgccgtatg gcggcgtgaa ggattcgggg ctcgggcgcg agggcgtgcg ctacgcgatc
     3661 gaggatatga ccgagatgcg gttgatggtg atgcgcgaga cgtggtgagc cgtg
//
LOCUS       DATASH010000714         3529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2688184,
            whole genome shotgun sequence.
ACCESSION   DATASH010000714 DATASH010000000
VERSION     DATASH010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2688184"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VHV99_18920"
     CDS             <1..400
                     /locus_tag="VHV99_18920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX3637519.1"
                     /translation="HGTEAEEHRKVGRPWGFYDSLERGERFQVKRIVVKPGGRLSLQM
                     HHHRAEHWIVVRGTARVTRGEESFLLSENQSTYIPLGVTHRLENPGKTPLEMIEVQSG
                     SYLGEDDIVRLDDQYGRDQESDHAGAGTSR"
     gene            375..965
                     /locus_tag="VHV99_18925"
     CDS             375..965
                     /locus_tag="VHV99_18925"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX3637520.1"
                     /translation="MQARVLHADRRQEGGGAALHEPELNVSRFDERRFRHALGRFATG
                     VVVISTGSGEAMHAMTANAFMSGSLKPPLIVVSVGHRARMHERLMTSAVFGVSVLSDA
                     QEPHSRHFAGEPQSRLAPRFAPVDGLPDVVLLEHAAARFAARLVDRHPCGDHTLFVGE
                     VLVFSLDEHAPLIFFGGRYASVAGPTLDAPGAASPR"
     gene            1283..2002
                     /locus_tag="VHV99_18930"
     CDS             1283..2002
                     /locus_tag="VHV99_18930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEX3637521.1"
                     /translation="MEQANKDRSLVSKVMDGLVTGIVEEKYGAILPPQDVLSKEFDVS
                     RTVMREALSMLLARDMLDVRPKIGTRIRPMSDWRMIDEDVVNWRFRAKPDPLFLRDVI
                     EFRILIEPRASAQAAARGSAADIAAIREAFDAFRAIRPGEPGYQEADELFHERIMTAS
                     GNQFFKQMAAIIRGALSTVNPILGQKEGAWEQAVGTHQRVVEAIERRDPKEAEIASLA
                     MIDYSAEEIGSGITPAPPLRG"
     gene            2189..3187
                     /locus_tag="VHV99_18935"
     CDS             2189..3187
                     /locus_tag="VHV99_18935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3637522.1"
                     /translation="MQEAMQQPPSGEVIRWGIVGTGRIARRFALGLDHVPHAQLTALW
                     SRRAEPAMAFAGEFGGEVCGSFDALLASGIDALYIATVQDSHADYAIAALHAGLHVLC
                     EKPATINKPQLERVLEAARAAQRLFMEAMKPPFYPLYRKLRAHLEAEPIGEVGLVRAG
                     CSVPGVPDDHPSLSFEHAGGALLDIGVYEMFLAVDWLGAPREVQTLGRLGSTGVDTFA
                     SLNSQHERGIAQLFCGLDLHGKGDALLMAKGGHVTIHEPWWNPARATIRYADGHVVEL
                     DEPFEGGGLNYETAHFCELIRAGQIESPLMPHDKSLQMIEMADAARAALGLKFAGE"
     gene            3423..>3529
                     /locus_tag="VHV99_18940"
     CDS             3423..>3529
                     /locus_tag="VHV99_18940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX3637523.1"
                     /translation="MRMLGKLWHDNRSLVAFLFLMVLFRSAVADWNVVP"
BASE COUNT          633 a         1070 c         1205 g          621 t
ORIGIN      
        1 gcacggcacg gaggccgagg agcatcgcaa ggtcggccgg ccgtggggct tttacgattc
       61 gctcgaacgt ggcgagcgct tccaggtgaa gcgcatcgtc gtcaaaccgg gtgggcggct
      121 gtcgttgcag atgcatcatc atcgcgcgga acattggatc gtggtgcgcg gcaccgcgcg
      181 tgtcacgcgc ggcgaggaat ccttcctgct ctcggaaaac cagtcgacgt acattccgct
      241 cggcgtcacg caccggctcg aaaatccggg caagacgccg cttgaaatga tcgaggtgca
      301 atcaggcagt tatctcggcg aggacgacat cgtgcgcctc gacgatcaat acgggcgaga
      361 tcaggagagc gatcatgcag gcgcgggtac ttcacgctga caggcggcag gagggcgggg
      421 gcgcggcgtt gcatgagccg gagctgaacg tatcgcgctt cgacgagcgc cgtttccgcc
      481 atgcgctggg gcgtttcgcc accggcgtgg tggtgatttc gaccggcagc ggcgaggcga
      541 tgcacgcgat gaccgccaat gccttcatgt ccggttcgct caagccgccg ttgatcgtcg
      601 tgtcggtggg gcaccgcgcg cgcatgcacg agcgtctgat gaccagcgcg gtgttcggcg
      661 tgagcgtgtt gtccgatgcc caggaaccgc atagccgcca ctttgccggc gagccgcaga
      721 gccgcctcgc gccgcgcttc gcgccggtgg acggcctgcc cgacgtggtg ctgctcgaac
      781 acgccgcggc ccgtttcgcg gcgcgcctgg tggaccggca tccgtgcggc gatcacacgc
      841 tcttcgttgg cgaagtgctg gtgttctcgc tcgacgaaca cgcgccgctg atctttttcg
      901 gcggacgcta tgcgtcggtg gccggcccga cgctcgacgc cccaggcgcc gcctcgccgc
      961 gctagtcttt tcgcgccgga cccaggctgt tcacgccgtg tgcatcagac aattcggttc
     1021 gctacgtgcg tcctcgcact atgcaaattc cggaacggag gcacgcttga gacgcaagct
     1081 tatggcccgt caaaacatgc ggcgttacac ggaagaaaca attcttcaga gggataaccc
     1141 tctcaggagg tttcccaact ggcgcatctg gtttgaagct agcaacgtat cgtaaaagaa
     1201 agactaccca tcgataacga cacactaggg ccgcttgatc aacaggctac gcgggggcaa
     1261 acaccgcttc ggggagagtg tcatggaaca ggcaaacaag gatcgctcgc tggtcagcaa
     1321 agtcatggat ggacttgtga ccggcatcgt ggaagagaag tacggcgcca tactgccacc
     1381 gcaggacgtg ctgtcgaagg agttcgacgt cagtcgcacc gtcatgcgcg aggcgctgtc
     1441 gatgctgctc gcgcgcgaca tgctggacgt gcggccgaag atcggcacgc gcatccggcc
     1501 gatgagcgat tggcgcatga tcgacgagga cgtcgtgaac tggcgttttc gcgcgaaacc
     1561 cgatccgctg tttctgcgcg acgtgatcga atttcgcatc ctgatcgaac cgcgcgcctc
     1621 ggcgcaggcg gccgcacggg gcagtgccgc cgacatcgcg gcgattcgcg aggcgttcga
     1681 cgcgttccgc gcgatccggc cgggcgagcc gggttatcag gaagccgacg aactgttcca
     1741 tgagcgcatc atgacggcga gcggcaacca gttcttcaag cagatggcgg cgatcattcg
     1801 cggcgcgctg tcgaccgtca atccgatcct tggtcagaag gaaggcgcgt gggagcaggc
     1861 ggtgggcacc catcagcgcg tggtcgaagc gatcgaacgg cgcgatccga aggaagcgga
     1921 aatcgcctcg ctggcgatga tcgactacag cgcggaagag atcggcagcg gcattacgcc
     1981 ggcaccgccg ctacgcggct gagcgcggcc gggtgggacc gtgtgtgtgg cccgaatgtg
     2041 gccattacgg cggcgaccca ggttgtgttc cggcagggaa cttgcatgac gaacggcgcg
     2101 gcagtatggc agcgcatgca cgcgcggctt gcttcacaat agcgcctgtt ctgaactgca
     2161 cggacttgaa ggaacgatga ccatcgcaat gcaggaagca atgcaacaac ccccctcggg
     2221 cgaggtgatt cgctggggga tcgtcgggac gggccgcatt gcccgtcgct tcgcgcttgg
     2281 actcgaccat gtgccgcatg cgcaactcac cgcgctgtgg tcacgccgcg ccgaaccggc
     2341 catggctttc gccggggaat tcggcggcga ggtatgcggg agtttcgacg ccctgctcgc
     2401 gagcggcatt gacgcgcttt atatcgccac cgtccaggac agtcacgccg attatgcaat
     2461 cgccgcgctg cacgccggtt tgcacgtgct gtgcgaaaaa cccgccacca tcaacaagcc
     2521 gcaactcgaa cgtgtgctcg aggccgcgcg cgccgcacaa cggcttttca tggaagccat
     2581 gaagccgccg ttctatccgc tctacagaaa gttgcgcgcg catctggaag ccgagccgat
     2641 cggcgaggtc ggtctcgtgc gggccggctg ttcggtgccg ggcgtgccag acgatcatcc
     2701 gtcgctgtcg ttcgagcatg cgggcggcgc gctgctcgat atcggcgtct atgagatgtt
     2761 tctcgcggtc gactggctcg gtgcgccgcg tgaggtacag acgctcgggc ggctgggctc
     2821 gaccggcgtc gatacgttcg cgagcctgaa cagccagcat gagcgtggca tcgcccagct
     2881 gttttgcggg ctcgatctgc acggcaaggg cgacgcgttg ctgatggcca agggcggcca
     2941 cgtcacgatt catgaaccct ggtggaatcc ggcacgtgcg acgatccgct acgccgacgg
     3001 tcatgtggtc gaactcgacg agccgttcga aggcgggggg ctgaactacg agaccgcgca
     3061 tttttgcgaa ctgattcggg caggacagat tgagagcccg ctgatgcccc atgacaagtc
     3121 gctgcagatg atcgaaatgg ccgatgccgc gcgggctgct ttggggctta agttcgcggg
     3181 ggagtgacgg gtcgctggga gcgactgcgc gcgattcagg gcggcaagcg caggagtgcg
     3241 gcgagggcgc acgcgcgacg ctggggcagc aatgcgcacg gggcggcttg agcgaggcaa
     3301 ggcgtcgcag tcgccgcgcc gcttccagcg gcggtggtac gatctgcgcg ggcgcgcgga
     3361 ctcgaaccaa aagggccgcg tacccactgc tttcgattcc ccttccgctg gagccacttg
     3421 cgatgcgcat gcttggaaaa ctgtggcacg acaacaggag ccttgtcgcc tttctgttcc
     3481 tgatggtgct gtttcgcagc gccgtggccg actggaacgt cgtgccgag
//
LOCUS       DATASH010000715         2703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3676502,
            whole genome shotgun sequence.
ACCESSION   DATASH010000715 DATASH010000000
VERSION     DATASH010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3676502"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            299..1441
                     /gene="glnL"
                     /locus_tag="VHV99_18945"
     CDS             299..1441
                     /gene="glnL"
                     /locus_tag="VHV99_18945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HEX3637524.1"
                     /translation="MVLKNLMKARKGHVQTLSDDVQLVGSGLLPGFEALPTVVLVLEK
                     RTLRVAFANPSAESMLDLSRKQLTQMAWPDIFSNADELVATIAAIAAHRFHATHLDTI
                     LERPGHEPLHVHAIVGFLESAQDYVLLELFENERHLRTDREERINDLTAVNKQLIRNL
                     AHEIKNPLGGIRGAAQLLEFELGERQRDELREYTQVIIKESDRLQTLVDRLLEPHRHP
                     HIVGDVNIHEVCERVRQVILAEFPRGLTIERDYDVSVPDLRGDKEQLIQALLNIVRNA
                     AEALRERISQGDARIELRTRIARKITVSKRLCKLALDLRITDNGPGIPEEIRDRIFYP
                     LVSGREDGSGLGLTLAQTFVQQHDGLIEVESRPGHTEFQILLPLDS"
     gene            1588..>2703
                     /gene="ntrC"
                     /locus_tag="VHV99_18950"
     CDS             1588..>2703
                     /gene="ntrC"
                     /locus_tag="VHV99_18950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HEX3637525.1"
                     /translation="MKPIWIVDDDQSIRWVLEKALARENFATRSFANVREASAALDHD
                     SPQVLVSDIRMPGGSGLELLQTVRDKVPGLPVIIMTAFSDLDSAVAAFQGGAFEYLAK
                     PFDVDKAVELIRRAVDESMRGEQTWDDRVADAPEMLGQAPAMQDMFRAIGRLSHSAAT
                     VLITGESGTGKELVARALHRHSPRANGPFIALNTAAIPKDLLESELFGHERGAFTGAQ
                     AMRQGRFEQAENGTLFLDEIGDMPFDLQTRLLRVLSDGQFYRVGGHNPLRANVRVIAA
                     THQNLESRVRQGLFREDLYHRLNVIRLRLPALRERSEDIPLLTRHFLQKSARDLGVEP
                     KRVSEEALAYLASLPFPGNVRQLENLANWLTVMAPAQT"
BASE COUNT          543 a          809 c          834 g          517 t
ORIGIN      
        1 tcgatgctcg acgcgtacat cgaactgaag acgggcgagc tgcaacgcta tcgtcagtcg
       61 gtgcacccga tcgaattcga aatgtactac tcgctgtaag cggcaatgac gcttcgccct
      121 tcaaacggcg aagcgcttgc ccgacgctcg cgatcggtca cgaagggacg gcggcgccgt
      181 cccttttttg ttggacccga atgagtccac tcatgacgtt tgcggcgtaa gggcaatgaa
      241 gcaggacaat aagcggtaca cctgatctat caccatctcc tatcggccag actgcaagat
      301 ggttctgaag aatctgatga aggcaaggaa agggcacgtg cagacgttgt cggacgacgt
      361 tcagctcgtg ggttccggtt tgctgccggg cttcgaggca ttgccgacgg ttgtgctggt
      421 gctcgaaaag cgtacgctgc gcgtcgcgtt cgcgaacccg tcagcggaat cgatgctgga
      481 cctctcgcga aagcaactca cgcagatggc gtggccggac attttctcga atgcggacga
      541 attggtcgcg accattgccg cgatcgccgc ccaccggttt cacgcaacgc atctggacac
      601 catactcgaa cgtccaggcc acgaaccgct gcatgtgcat gcgatcgtgg gttttctgga
      661 aagcgcgcaa gactatgtgc tgctcgaact gttcgagaac gagcggcatt tacgcaccga
      721 ccgcgaggag cgcatcaatg acctcacggc cgtcaacaaa caactgattc gcaatctcgc
      781 gcatgagatc aagaacccgc tcggcgggat tcgcggcgcg gcgcaattgc tcgagttcga
      841 actcggcgag cgccagcgcg acgagttgcg cgagtacacg caggtcatca tcaaggaatc
      901 ggaccggctg caaacgctgg ttgatcgctt gctcgagcca caccgtcatc cgcatatcgt
      961 cggcgacgtg aatattcacg aagtctgtga acgtgtgcgc caggtgatcc tcgcggagtt
     1021 tccgcgtggc ctcacgatcg agcgcgatta cgacgtgagt gtgccggacc tgcgcggcga
     1081 caaggaacag ctgatccagg cgctgctgaa catcgtacgc aacgctgccg aagcgttgcg
     1141 cgaacgcatc tcgcaaggcg acgcgcgcat cgaattgcgc acgcgtattg cgcgcaagat
     1201 caccgtgtcg aaacgcttat gtaagctggc actggacttg cgtatcactg acaacggccc
     1261 aggcattccc gaggaaatcc gtgatcgcat tttctatccg ctcgtgtcgg ggcgtgaaga
     1321 cggcagcggt ctcgggttga cgcttgcgca gactttcgtg caacagcacg acggcctgat
     1381 cgaagtggaa agccggccgg gccacaccga gtttcagatt ctgctgccgc tcgacagcta
     1441 acagcgaatg caccgcgaga aaacgccggg ccgccccagg ttttccgccc cgcaggacgt
     1501 ccccttgggg gatcaccccc ctcggggggc ctggcgcgaa gcaacaggtt gggggcgttt
     1561 tccctcttaa gacttctgac cacctatatg aagccgatct ggatagtaga cgacgatcaa
     1621 tcgattcgct gggtgcttga aaaagcgctc gctcgcgaaa acttcgcgac acgcagcttt
     1681 gcgaacgtgc gcgaggcttc cgcggcgctc gatcacgaca gccctcaggt gctggtgtcc
     1741 gacatcagga tgcccggcgg ctccggcctt gaattgctgc aaaccgttcg cgacaaggta
     1801 ccgggcttgc ccgtcatcat catgacggcg ttctccgatc tggatagtgc ggtcgccgcg
     1861 ttccagggcg gtgcgttcga gtacctcgcc aaaccgtttg acgtcgacaa ggcggtcgaa
     1921 ctgatccggc gcgcggtcga cgaaagcatg cgcggcgaac agacgtggga cgaccgcgtc
     1981 gccgatgcac cggaaatgct cggccaggcg ccggcgatgc aggacatgtt tcgcgcgatc
     2041 ggccggctgt cgcattccgc ggccaccgtg ctcattaccg gcgaatcagg caccggaaag
     2101 gaactggtcg cgcgtgcgtt gcaccgacat agcccacgcg cgaacggtcc cttcatcgcg
     2161 ctgaatacgg cggcgattcc gaaagatctg ttggaatccg aattattcgg tcatgagcgt
     2221 ggtgcgttta ccggcgcgca ggccatgcgc cagggacgct tcgaacaggc cgagaacggc
     2281 acgctgtttc tcgatgaaat cggcgatatg ccgttcgatt tgcagacgcg attgttgcgt
     2341 gtgttatcgg acggccagtt ctaccgcgtc ggcggccaca atccgttgcg cgcgaatgtg
     2401 cgcgtgatcg cggcgacgca ccagaacctc gaatcgcgtg tgcgtcaggg tctgttccgc
     2461 gaggacttgt atcaccggct caatgtgatc cgcttgcgct tgccggctct gcgcgagcgc
     2521 agcgaagaca ttccgctgct cacgcgccac ttcctgcaaa aaagtgcgcg cgatctgggc
     2581 gtggagccga agcgggtgtc cgaagaggcg ctggcttatc tggcatcgct gccgtttccc
     2641 ggcaacgtgc ggcaactgga gaatctcgcc aattggctca cggtgatggc gccggcgcag
     2701 acc
//
LOCUS       DATASH010000716         4587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2490679,
            whole genome shotgun sequence.
ACCESSION   DATASH010000716 DATASH010000000
VERSION     DATASH010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2490679"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VHV99_18955"
     CDS             complement(<1..651)
                     /locus_tag="VHV99_18955"
                     /inference="COORDINATES: protein motif:HMM:NF019149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX3637526.1"
                     /translation="MHVPIGYVRTVDDRLEKDPDERVRAAIDLVFRKFAELGSARRVY
                     FWLGEQGIEMPAVTSAGSAERHEWRAPRYHSVLSLLHNPIYAGAYTHGRSKTSVRIED
                     GRKRAVRTHQHRPEDWAVLIRDHHEGYIDWDVYCANRELMAHNTNGHGSAVRGSIRSG
                     SALLSGLLRCGHCGAKLSVNYPGPTSIRYQCTTHILSRDHACCVMFGGLGADQLVAE"
     gene            complement(688..1140)
                     /locus_tag="VHV99_18960"
     CDS             complement(688..1140)
                     /locus_tag="VHV99_18960"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX3637527.1"
                     /translation="MNKITAEHLSRLACVYVRQSTQGQVQNNLESQRRQYGLVERARQ
                     LGWERVEVIDDDLGRSGSGTLRPGFERLLGLLCDGEVGAVLSIEASRLARNGRDWHTL
                     LEFCSVVGALLIDAEGIYDPVQMNDRLLLGMKGTISEMELASFRQRAH"
     gene            complement(1121..1294)
                     /locus_tag="VHV99_18965"
     CDS             complement(1121..1294)
                     /locus_tag="VHV99_18965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637528.1"
                     /translation="MPPRPMVQIALFEDASAVPLPTLPQDVQLQLQQQMVQWIRAIAV
                     AINQGEDDEQDHR"
     gene            1601..2095
                     /locus_tag="VHV99_18970"
     CDS             1601..2095
                     /locus_tag="VHV99_18970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637529.1"
                     /translation="MFKRLKHRLRLEAVTGLDYLALQQDLGAKILIDNLCTLLSELDA
                     PHDEECASRPNRVYAPGALKPILGACLLRVRRCLPSLVGVLAVIHQTRCRIQPSRSYP
                     RPPRKAKPHFHSRVQTRLMVRGLKLRALLIGVAVIRISAQRQISTLALPRVSASGQFY
                     EVEI"
     gene            2325..3386
                     /gene="rfaE1"
                     /locus_tag="VHV99_18975"
     CDS             2325..3386
                     /gene="rfaE1"
                     /locus_tag="VHV99_18975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HEX3637530.1"
                     /translation="MTRNPPVKLSPPDFDCAQLIVVGDVMLDWYWHGSTSRISPEAPV
                     PVVHVQTEETRVGGAGNVALNAAALGARVRLLGLAGQDAHADRIEELLREGGVDCVLQ
                     RVAGSKTITKLRILSRHQQMIRADFEDHFPERNGDELIFSFERQLHEVDAVVLSDYAK
                     GSLHDSKNLISAARRAGKPVIVDPKGTDFERYRGATVITPNFSEFEAVVGRCESDATI
                     ERKGAALCDAFELEAVLVTRSEKGMTLIARGHAPLHLPTRAREVFDVTGAGDTVVATL
                     SAALGAGMPLDEAVVLSNVAAGIVVAKLGTATVRRPELQQALQHDLHSARRGNILARI
                     SLLPWRTKRRSDKPTSVGT"
     gene            3498..>4587
                     /locus_tag="VHV99_18980"
     CDS             3498..>4587
                     /locus_tag="VHV99_18980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX3637531.1"
                     /translation="MKQLVEPSNARREEAAVEAALPADASSAQYAQWQGPSAGFAING
                     KFASQRITGVQRVGYELAMAFQRLFPDETDLPLLIPANARTDVVLPKAKMIGRWLKGS
                     LWEQLALPFAIGGRTLLSLCNMGPLFARRQVVMMHDVAIYDLPENYSWKYRLWYRVAF
                     SLLKRNASHIVTVSEFSKTRIMERLGIDTSRISVVLNGVDHFEKIAPDAAILSRLNLQ
                     HDGYVLAVGNLSAGKNLPRIVAAMERLSDCHDLKFVVVGGCDLRVFSSQAKVGYDLTK
                     NIIPAGFVSDGELRALYENAACFLFPSLYEGFGLPPLEAMSCGCPVIVSREASLPEVC
                     GNAAMYCDAHSVDDIVDKVTQMMEDAAVR"
BASE COUNT          903 a         1344 c         1366 g          974 t
ORIGIN      
        1 ctcggccacc agttgatccg cgccgaggcc gccgaacatg acacagcagg cgtggtcacg
       61 gctgaggata tgtgtggtgc attgatagcg gatgctggtc gggcctggat agttcacgct
      121 aagcttggcc ccacaatgcc cacagcgcag tagtcctgat aataacgcgc tgccgctcct
      181 gatcgagccg cgcaccgcac tgccatgtcc attggtgttg tgggccatca actcccggtt
      241 ggcgcagtac acgtcccagt cgatgtagcc ctcgtgatga tcgcgaatca gcaccgccca
      301 atcctcgggc ctgtgttggt gggtacgcac ggcacgtttg cgcccatcct caatgcgcac
      361 gctggtcttg ctgcggccat gtgtataagc acccgcgtag atcggattgt gcagcaggct
      421 cagcacgctg tggtagcgcg gggccctcca ctcgtggcgc tctgcgctgc cggcgctcgt
      481 cacagccggc atctcgatac cttgctcgcc cagccagaaa tacacgcgcc tggcgctgcc
      541 gagttcggcg aatttgcgaa acaccaagtc gatggccgcg cgcacgcgct cgtcgggatc
      601 cttctcgagg cgatcgtcga cggtgcgtac gtagccgatg ggcacatgca tgtaaagctc
      661 tccgcgttta gccttctgct tcatcgctta gtgcgctcgc tgccggaagc tggccagttc
      721 catctcgctg atcgtgcctt tcatgccgag cagcagccgg tcattcatct gcacgggatc
      781 atagatgcct tctgcatcga tgagcaacgc ccccacaacg ctgcagaact ccagcagcgt
      841 gtgccagtct cgcccgttgc gtgccaggcg cgaagcttcg atgctcagaa ccgcgccgac
      901 ctcgccatcg caaagcaagc ccagcaaccg ttcgaagccc gggcgcagcg tgcctgaacc
      961 ggagcggccg agatcgtcgt cgatgacctc aacgcgctcc caccccaatt gccgagcgcg
     1021 ctcaaccagc ccatattgcc gacgctggct ctcgaggttg ttctgcacct ggccctgcgt
     1081 cgattggcgg acatatacac aggctagccg cgacagatgt tcagcggtga tcttgttcat
     1141 cgtcttcccc ctggttgatc gccacggcga tggcccgtat ccactgcacc atctgctgct
     1201 gcagttggag ctgaacgtcc tgcggtagtg tcggcaaggg gactgcgctg gcgtcttcga
     1261 acagcgctat ctgaaccatc gggcggggcg gcatggagtt ctcctgttgg gctcgggcta
     1321 tcccgtaagg ataagaacgt cgatacacaa cggcgaagct ccagtagcgc tgcaatggac
     1381 aagcgcgatt gctcgacgac tcgcacattg gcggccgcca gctcggtcat ccacgcgggg
     1441 acggacaccg tcgtgccatt agagcgccgg atgacgtagc agggctcatc gttgacgtaa
     1501 tgcagccgga ccaccgcgag acgctcaccc acaagggggt gaaagcggta gcgcactaag
     1561 atgtcatgcg agagatgttg acgggcagaa tgacttaccc ttgttcaagc gcctcaagca
     1621 ccggctgcga ctggaagccg tcacggggct ggactacctg gccttgcagc aggacctcgg
     1681 tgcaaaaatc ctcattgata acctctgcac actgctcagc gagcttgatg cgccgcacga
     1741 cgaggagtgc gccagtcgtc ctaaccgggt gtacgcaccg ggagcgctca agcccatcct
     1801 cggcgcgtgc cttctgcgcg tccggcgctg tctgcccagc cttgtcggtg tgctggctgt
     1861 gatccaccag acccgatgcc ggatacagcc ctcgcgctct tatccaaggc cacccagaaa
     1921 agccaagccc cacttccatt ctcgcgtaca aactcgcttg atggtccggg ggcttaagtt
     1981 gagagcattg ctgatcggtg tcgcagtgat ccgcataagc gcgcaaagac agataagtac
     2041 gctcgcgctg ccccgagtgt ccgcctctgg acaattctac gaagtggaga tatagacagt
     2101 gcgtggtata tcaatcgacc tttctacgcg agtctttcta cggtgatatt tgcatcacga
     2161 agcaacgcgc gcatctatga tctgttagat ggcctagacg aaattgtcat agtcaaacct
     2221 cgggttcgaa tatcccagaa atgcaaagta tgtgatggga aaaataagcg tcgcttctgt
     2281 cgccgacgtt ttgagtgaag tttattcgat caatctaggc tgccatgaca agaaacccgc
     2341 cggtgaagct ttcgcctcct gatttcgact gcgctcaatt aattgtggtg ggcgacgtca
     2401 tgctcgattg gtactggcac ggctcgacct cgcggatctc gccagaggcg cccgttcctg
     2461 tcgtccatgt tcaaaccgaa gagacgcgtg tcggtggggc gggaaacgtc gcgctgaacg
     2521 cggcggcact tggagctcgc gttcgtcttc tcggcttggc cgggcaggat gcgcacgccg
     2581 accggatcga agagttgctg cgcgaaggcg gggttgactg cgttctccaa cgcgtcgcgg
     2641 gcagcaagac gatcacgaag cttcgcattc tttcccgcca ccagcagatg atcagggcgg
     2701 actttgagga ccactttccc gaacgcaatg gagatgagtt gatcttcagc tttgagcggc
     2761 agttgcacga agtcgatgcc gtggtcttat ccgactacgc caaaggctca ctgcacgatt
     2821 cgaagaacct gatctccgct gcccggcgtg caggaaaacc cgtgatcgtc gatccgaaag
     2881 gaacagattt cgagcgttat cgcggcgcca ctgtcattac gcccaacttt tccgagttcg
     2941 aggcagtcgt cgggcgttgc gagagtgacg ccactatcga acgaaagggg gccgcccttt
     3001 gcgacgcgtt tgaactcgaa gcagtcctcg tcacccgcag cgagaaaggc atgacattaa
     3061 ttgctcgcgg ccatgccccg ttgcatttgc cgacgcgcgc gcgtgaggtg ttcgatgtga
     3121 cgggcgcggg ggacaccgtc gttgccacgc tgagtgctgc tctcggtgcc ggcatgccgc
     3181 tggacgaagc agtcgtgctt tccaatgtcg cggcgggcat cgtcgtggct aaattgggaa
     3241 ccgcgacggt ccgccggcct gagcttcagc aggcactaca gcatgacttg cattccgcac
     3301 gtcgaggcaa tattctcgcg cgaatttccc tattaccctg gcggacgaaa cgccgctccg
     3361 ataagccgac tagtgtgggc acctgacgcg gcgattccgt gagcggcatg tcgaagactt
     3421 gcacggtgtg gacaacttgc actggctggc agcccttctc gacactcaca tcgactagca
     3481 tgctggagcg atatctgatg aaacagttag tggagccgtc caacgcgcgg cgggaagaag
     3541 ccgctgttga ggctgcgctg ccggccgatg ctagctcggc acagtacgct cagtggcagg
     3601 gtccctctgc ggggtttgca atcaatggca agttcgcgtc tcaacgcata acaggggtgc
     3661 aacgtgttgg ttatgaactg gcgatggcgt tccagcgcct gtttcctgac gaaaccgacc
     3721 tgcccttgct gattcccgcg aacgcacgaa ccgacgtagt cttgccgaaa gcaaaaatga
     3781 ttggccggtg gttgaagggt tctctgtggg agcaactggc actgccattc gcgattggcg
     3841 gccgaacgct tctcagcctt tgcaatatgg gccccttgtt cgctcgccgg caggtcgtga
     3901 tgatgcatga cgttgcgatc tacgatctgc cggaaaatta ctcgtggaag tacaggctct
     3961 ggtatcgcgt cgcgttttcg ctgctgaagc gcaacgctag tcacatcgtg acggtgtcag
     4021 aattctcgaa gacacgaatc atggagcgcc ttggcatcga cacgtcgcgc atttcagtgg
     4081 tcttgaatgg cgtggaccac tttgagaaga tcgcaccgga cgcggcgatt ctgtctcggt
     4141 tgaacctgca gcacgatggt tacgtgctcg ctgtcggaaa tctctcggca ggaaagaacc
     4201 ttccacgcat agttgccgcg atggagcgcc tgagtgactg ccatgacttg aagtttgttg
     4261 tcgttggcgg atgcgatttg cgcgtcttca gttcacaggc gaaggtgggt tacgacttga
     4321 cgaaaaatat catccccgcg ggcttcgtat cggatggcga attgagagcg ctgtacgaaa
     4381 atgcagcctg ctttctgttt ccttcgttat atgagggctt cggattgccg ccgcttgaag
     4441 cgatgtcctg tggatgtcct gtcatcgtgt cacgtgaagc ttcgcttccc gaggtatgcg
     4501 ggaatgctgc gatgtactgt gacgcgcatt cggtcgacga catcgtcgac aaagtgacgc
     4561 agatgatgga agacgccgcg gtgcgcg
//
LOCUS       DATASH010000717         2465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5258408,
            whole genome shotgun sequence.
ACCESSION   DATASH010000717 DATASH010000000
VERSION     DATASH010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5258408"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..1373
                     /locus_tag="VHV99_18985"
     CDS             117..1373
                     /locus_tag="VHV99_18985"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3637532.1"
                     /translation="MTLIIAFMDRTNIGFAIPSMGKELALTSSILGFASGVLFLGYGA
                     SQVLGGWIADKGYGKVLIAVLMVLWGVIEMAQGFVHNAQQLVAVRFAIGVFEGGIFPT
                     FLLFVRNWFAPSERARANGIWQLCYPFAAMASGPIAGYILKLGDWRTLFIVEGVFPLV
                     WVFVWLWGVADTPRNAKWLKAADRAQLIEHLDSENKRHASQAPLPTGVSFSSQLRRPA
                     VWLFATAILLWNTGFLGFVIWLPSVIHQQPGLSPIDIGWLSSIPYAFAIVLMPILTYW
                     SDRKLDRRLFSAIPLAISGLALLVGGEIFESSPFVVNMALLVIAGATLYGSQPVLWSI
                     ATDLVPPEVTARISGMINGIGMIGAFGGPYFVGFARSLTNSFSAGLLVMGLCLIGTSA
                     FVMLIKETARPGRQRDTTVEPNMGRS"
     gene            complement(1508..2410)
                     /locus_tag="VHV99_18990"
     CDS             complement(1508..2410)
                     /locus_tag="VHV99_18990"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007731214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3637533.1"
                     /translation="MKIGFVGLGAMGAAIARNLVDAGHDVQVLNRSPARAEPLREAGA
                     SVASSPAQAALGADVVFTMVADDAAHAEVTFGKAGIATTLKPGALHISMSTISVATAQ
                     DHAAKYREAGLAFVSAPVFGRPDAAAARKLFIMAAGADEHLKVAVPLLETLGQSVGIV
                     GEDPSQANLVKLIGNFMLSVIIETLGEACAVAGKAGIDPMRLVELLTSANFNAPPYKI
                     YGPLIAAQKFQPPGFSLPLGQKDNRLMLAAAEALGVPLPLASLVHDRFLTLRSQGLGA
                     DHDWSAVALCALSDAGLSNALHRS"
BASE COUNT          437 a          785 c          746 g          497 t
ORIGIN      
        1 atacaaagat atacgtgtgc tgtggcgcag ggctagttgc cgcctcgtcg ccatgcaccc
       61 gaacatggag acagacgatg aagcaaagat ggtttcgctt actgcccgtg atgatgatga
      121 cacttatcat cgcattcatg gaccgcacca acattggctt cgcgattccg tcgatgggca
      181 aggagctggc gctgacctcc tcgattctcg gattcgcgtc aggcgtgctg tttctcggct
      241 atggcgcttc gcaggttctg ggcggctgga tcgccgacaa gggctacggc aaggtgctga
      301 tcgccgtgct gatggtgctg tggggagtga tcgaaatggc gcagggtttc gtacacaatg
      361 cgcagcagct ggtcgcggtt cgcttcgcga tcggcgtatt cgaaggtggc atattcccga
      421 ctttcctgct gttcgtaagg aactggtttg cgccgtccga gcgtgcccgc gcgaatggca
      481 tctggcaact ttgttacccg ttcgccgcga tggcgagcgg tcctatcgcc ggctacatcc
      541 tgaagctcgg cgactggcgc acgttgttca tcgtcgaggg cgtgttcccg ctcgtatggg
      601 tatttgtgtg gctgtggggc gtggccgata cgccgcgcaa cgcgaagtgg ctgaaagccg
      661 ccgatcgcgc gcaactgatc gaacacctcg actcggaaaa caagcggcac gcgtcgcaag
      721 cgccgttgcc gacaggcgtg tcattcagct cgcaattgcg gcgtccggcg gtgtggctct
      781 tcgcaacggc gatcctgctt tggaacaccg gcttcctcgg ctttgtgatc tggctgccca
      841 gcgtgatcca tcaacagccc ggcctttcgc caatcgacat cggctggctc tcatcgattc
      901 cctacgcgtt cgcgatcgtg ctcatgccga tcctgactta ctggtccgac cgcaaactgg
      961 accggcggct cttttccgcg ataccactgg ccatttccgg cctggccctg ctggttggcg
     1021 gcgagatctt cgaatccagt ccgttcgtgg tgaacatggc gctgctcgtg atcgccggcg
     1081 cgacacttta cggatcgcag cccgttctct ggtcgatcgc caccgacctc gtgccccccg
     1141 aagtgacggc ccgaatcagc ggcatgatca acggtattgg catgatcggg gcatttggcg
     1201 gcccttactt cgtcggcttc gcgcgctcgt tgaccaactc gttttccgcc ggactattgg
     1261 tcatgggcct ctgtctgatt ggcaccagcg cgttcgtgat gttgatcaag gaaaccgcga
     1321 gaccaggacg tcagcgcgat acgaccgtgg aaccgaatat gggtcgtagc tgagccctgg
     1381 catgcaccct gactcagcta cagctacagc tacagctaca gctacagcta cagcacgaca
     1441 ttcccgctcg cccgcggttc gacgcgtcgg atgccgctca tcgcgagcgg catcgtgccc
     1501 attgcgttca gctgcggtga agcgcgttac tcaagcctgc atcggacaac gcacacagcg
     1561 ccaccgccga ccagtcatga tcagcgccga gcccttgcga gcgcaacgtc aggaagcggt
     1621 cgtggacgag acttgctagc ggcagcggaa cgcccagtgc ctcagcggcg gccagcatca
     1681 gacggttgtc cttctggccg agcggcaacg agaagccagg cggctggaat ttctgcgccg
     1741 cgatcagcgg accgtagatc ttgtagggcg gcgcattgaa gtttgcgctc gtcaacagtt
     1801 cgaccagacg catcggatcg atgccggcct tgcccgcgac ggcacaggct tcgcccagcg
     1861 tttcgatgat cacggacagc atgaaattgc cgatcagctt caccagattg gcctgcgaag
     1921 gatcctcgcc gacaatgccg accgactgcc ccagcgtttc gagcagcggc acggcaacct
     1981 tgaggtgctc gtccgcgccg gccgccatga tgaacagctt gcgcgccgcc gcggcatcgg
     2041 gccggccgaa taccggcgcg gacacgaagg caagacccgc ttcacggtac ttcgctgcgt
     2101 gatcctgcgc ggtggcgaca ctgatcgtgc tcatcgagat gtgcagcgca cccggtttca
     2161 gcgtggtggc gatgcccgcc ttgccgaacg tgacctctgc gtgcgcggca tcgtcggcca
     2221 ccatcgtgaa caccacgtcg gccccgagcg ccgcctgcgc gggactcgac gcgacgctcg
     2281 cccccgcttc gcgcaacggt tcggcgcggg ccggactccg gtttaaaacc tgcacgtcat
     2341 gccccgcgtc gaccaggttc ctcgcgatcg cggcgcccat cgcgccaagt cctacaaatc
     2401 caattttcat aatgacattc caaatgatca gccgcgcgaa cgcgggcctg tttcagactg
     2461 cgaat
//
LOCUS       DATASH010000718         4393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1700298,
            whole genome shotgun sequence.
ACCESSION   DATASH010000718 DATASH010000000
VERSION     DATASH010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4393
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1700298"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..198
                     /locus_tag="VHV99_18995"
                     /pseudo
     CDS             1..198
                     /locus_tag="VHV99_18995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012400213.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
     gene            333..656
                     /locus_tag="VHV99_19000"
     CDS             333..656
                     /locus_tag="VHV99_19000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007741806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEX3637534.1"
                     /translation="MNYVVLILGMAVITWVIRAAVFVLGDRLVFPPLVRTALGFVPVT
                     VLTAIIVPMAVSPHGTSAELTWRNPQLVGALAAIAVSALTRRPLLTIAVGLTVFFVWQ
                     GVVLK"
     gene            1164..1925
                     /locus_tag="VHV99_19005"
     CDS             1164..1925
                     /locus_tag="VHV99_19005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018441548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4088 family protein"
                     /protein_id="HEX3637535.1"
                     /translation="MGQITLTLKDETIATLRKDFDAFLRVSLKLDPQFATPSFEDFLR
                     AKLLDNMVPLTEHAVQRMLQGGQYAWAKRTLDKEFPDVVAILIRQAAEFGFGFAARSE
                     WTPEELAKQCRDWAAAIVKEAEGDAALIDPLAAQIKSEAKDIQALEELMQTPAWRLVE
                     SLRQRVYEAKVACETSVGSSAREKLGELRGLLRLGISHGSFQKQEAQQIMEYLRLLKP
                     EIFVEEPYDVFSRLAAWLRNFFVPPRPAPQQQQPR"
     gene            complement(1951..4089)
                     /locus_tag="VHV99_19010"
     CDS             complement(1951..4089)
                     /locus_tag="VHV99_19010"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEX3637536.1"
                     /translation="MLAVAESPPPFDTAAWLVKLNDAQREAVEYGAGDPNAPPGALLV
                     IAGAGSGKTNTLAHRVANLVVKGADPRRILLLTFSRRAALEMTRRVTRIASAALGARA
                     ALAQGLTWSGTFHSVGARLLREYADLIGLAPAFTINDREDSADLMNLVRHELGLSAKE
                     RRFPAKGTCFSIYSRVVNTGASLADVLNSAFPWCREWEADLRMLFAAYVEAKQKQSVL
                     DYDDLLLYWSHMAAEPAIAADLSARFDHVLVDEYQDTNRLQASILLALKPDGRGLTVV
                     GDDAQSIYSFRGATVRNILDFPAHFNPPARQITLERNYRSTGPILAASNAVIELASER
                     YTKNLWTDKASAQRPRLVTVADEADQARYIVEQVLEAREQGMKLKSQAVLFRAAHHSA
                     ALEIELTRRNIPFVKFGGLKFLDSVHVKDVLAVLRWAENPRDRVAGFRVVQLLPGIGP
                     ATAAKVLDEIVTRAGAGNPADLTQSAGGALAAFAPPARAQEDWHPFVKLMSSVYGRQT
                     PWPAEFEMVRRWYEPHLERNHEDAAIRHADVLQMESIAGTYPSRERFLTELTLDPPDA
                     TSGESGVPLIDEDYLILSTIHSAKGQEWRNVFVLNGVDGCIPSDLGTGSEDEIDEERR
                     LLYVAMTRAKEDLHIVVPQRFYVHNQTHMGDRHVWASRTRFIPAHLMPLFDAYAWPRV
                     PVAGAPTAAGLAAAAQAKIEIGARLRKMWD"
BASE COUNT          776 a         1419 c         1428 g          770 t
ORIGIN      
        1 cagtcgctcg gactcgattt cgcgatggtg gccactttca ttgcgatcgt cgtgccgcag
       61 ctcgtcgcgc tgcgctatat cgcggcggcc gttaccgctg gcacgttggc attcttctgg
      121 caagcctggc catacaagct cggcctgctc ggcgcggtgt tcgccggcgt cgcaatcggt
      181 gtcttgctgt cagtgccgcg acttgggcga ggcacacggg ggagtcgcga aaccccgtgc
      241 gaaacctcgc aggagtccac acaggagcct ctgcgggatg ccccgcggaa aatctcgcag
      301 gaaacctcgc agaaaaccgt aggagcatcg cgatgaacta tgtcgtcctg attctcggta
      361 tggccgtcat tacgtgggtg atccgcgccg ccgtcttcgt gctgggcgac cggctggtat
      421 ttccgccgct cgtgcgcacc gcgctcggct tcgtgccggt tacggtgctg accgcgatca
      481 tcgtgccgat ggcggtctcg ccgcacggca ccagtgccga attgacctgg cgcaatccgc
      541 aactggtcgg ggcgctggcg gccatcgcgg tcagcgcgct gacgcggcgg ccgctcctga
      601 ccatcgcggt cggtctgacg gtcttttttg tctggcaagg cgtcgtgctt aaataggcgc
      661 aaaaggccac tattgacgca gcggggcagc cgcatttctc gcggccgtga cgcccgtgac
      721 ggcggctatg cccctctcag catcggccgc ggcatcgtcg cactgccaag gcgaggacga
      781 cttgcaacgg cggcgcgtgc tacatcgaca gggcgagcgc ataggccagc cacagcgcct
      841 cgcggcgacg cgtcgccccg ccgcacaccg gcgccgcctc gcatcggccg cgccgcgccg
      901 cgcgcgtatt gcctgcgctc cgtgcgcgat gcaggcgacg ccgacgcctg cctccagacc
      961 cgccgccagt ccttcatcaa cgctcaagtt tctctcaatt ccgtcgataa caagtcgagg
     1021 tccgctcatc gggatacgtc ccgctggtta cgcggcaaca ggcagaatca accgcgccgg
     1081 cattcggcgt cgcgtctcac aaggtccggg tgcggccggg tgacatgacg aagccgcgcg
     1141 ggcaggacca aacgggataa cacatgggtc aaattaccct cacgctcaaa gacgagacca
     1201 ttgcgacgct gcgcaaggac ttcgacgcct ttctgcgggt ttcgctgaag ctcgatccgc
     1261 agttcgcgac gccgtcgttt gaggattttt tgcgcgccaa gctgctcgac aacatggtgc
     1321 cgctgactga acacgcggtt cagcggatgt tgcagggtgg ccagtacgcg tgggcgaaac
     1381 gcacgctgga caaggagttt cctgacgtcg tcgcgatcct gatacggcag gcggcggaat
     1441 tcggtttcgg ctttgcggcc cgctcggaat ggacgccgga agagctcgcc aagcagtgcc
     1501 gcgactgggc cgcggcaatc gtcaaggaag cggaaggcga cgccgcgctg atcgatccgc
     1561 tcgcggcgca gatcaagagc gaggcaaagg atattcaggc gctcgaggag ctcatgcaga
     1621 cgccggcctg gcggctcgtg gaatcgctgc gccagcgcgt ctacgaagcc aaggtggcat
     1681 gcgaaacgag cgtgggcagc agcgcgcgtg aaaagctcgg cgaactgcgc gggctactgc
     1741 gtctgggcat ttcgcacggc tcgttccaga agcaggaagc gcagcagatc atggaatacc
     1801 tgcgcttgct gaagccggaa attttcgtcg aagagccgta tgacgtgttc tcgcgcctcg
     1861 ccgcctggct gcgcaatttc ttcgtgccgc cgcgtccggc gccgcaacag cagcagccca
     1921 gatagtcgcg gtaaggtcga gcagaagccg tcaatcccac atctttctga ggcgggcccc
     1981 aatctcgatc ttcgcctgcg ccgccgcggc cagccccgcc gctgtcggcg cacccgccac
     2041 cggcacacgc ggccacgcgt aagcgtcgaa cagtggcatc agatgcgccg gaatgaaacg
     2101 cgtgcgcgac gcccagacat gacggtcgcc catatgggtc tggttgtgca cgtaaaagcg
     2161 ctgcggcacg acgatatgca ggtcttcctt cgcgcgcgtc atcgcgacgt ataacaggcg
     2221 ccgctcttcg tcgatttcgt cctcgctgcc ggtgccgaga tcggacggaa tgcaaccgtc
     2281 gacgccattc agcacgaaca cgttgcgcca ctcctgcccc ttcgccgaat gaatcgtcga
     2341 caggatcagg tagtcctcgt cgatcaacgg cacgccggat tcgccgctgg tggcgtcggg
     2401 tggatcgagc gtcaattcgg tgaggaaacg ctcgcgcgac gggtaggtgc ccgcgatgct
     2461 ttccatctgc aacacgtccg cgtgacggat cgccgcgtcc tcgtgattgc gctcgagatg
     2521 cggctcgtac cagcgccgca ccatctcgaa ctcggctggc cacggcgtct ggcgcccgta
     2581 cacgctcgac atcagcttga cgaacggatg ccagtcttcc tgcgcacgag cgggcggcgc
     2641 aaaggcggcc agcgcgccac cggcgctctg ggtgagatcg gccggattgc cggcgcccgc
     2701 ccgcgtcacg atctcgtcaa gcaccttcgc ggcggtggcg gggccgatgc ccggcagcaa
     2761 ttgcacaacc cgaaaaccgg cgacgcggtc gcgcggattc tccgcccagc gcagcacggc
     2821 gagcacatcc ttgacgtgca ccgagtcgag aaacttcagg ccgccgaact tcacgaacgg
     2881 aatgttgcgc cgggtcagtt cgatctcgag cgcggcgctg tgatgggcgg cgcgaaacag
     2941 caccgcctgc gacttgagct tcatgccctg ctcgcgcgct tccagcacct gttcgacgat
     3001 gtagcgcgcc tgatccgctt cgtcggcgac cgtcaccaga cgcgggcgct gcgccgaggc
     3061 cttgtcggtc cacaggttct tcgtgtagcg ctcgctggcg agttcgatca ccgcgttcga
     3121 tgccgccaga atcggcccgg tggagcggta attacgttcg agcgtgattt gccgcgctgg
     3181 tggattgaag tgcgccggga agtcgagaat gttgcgcacc gtcgcaccgc gaaacgaata
     3241 gatcgactgg gcgtcgtcgc cgaccacggt caggccacgg ccgtcgggct tcaacgcgag
     3301 caggatcgac gcctgcaagc ggttggtgtc ctgatactcg tcgaccagca cgtgatcgaa
     3361 acgcgctgaa agatcggccg cgatcgccgg ctcggcggcc atatgcgacc agtagagcag
     3421 caaatcgtcg tagtcgagca cgctttgttt ctgcttggct tcgacgtacg cggcaaacaa
     3481 catgcgcaga tcggcttccc attcccggca ccaggggaat gcgctgttga gcacgtcggc
     3541 aagcgacgcg ccagtgttca ccacacgcga gtagatcgaa aagcaggtgc ccttggccgg
     3601 aaaacgccgc tctttcgccg acaagccgag ttcgtgtcga acgaggttca tcaggtcggc
     3661 ggaatcctcg cggtcgttga tcgtgaaggc aggcgcgagg ccgatcaggt ctgcgtattc
     3721 gcgcaatagc cgcgcgccga cgctatggaa agtccccgac cacgtgagcc cttgtgccag
     3781 cgcggcccgc gcaccgagcg ccgcactggc gatgcgcgtg acgcggcggg tcatttcgag
     3841 cgcggcgcgg cgtgagaagg tcagcagcag gatacggcgc ggatcggcac ccttcacgac
     3901 cagattggcg acgcggtgcg ccagtgtgtt ggtcttgccg gagccggcgc ccgctatcac
     3961 cagcaaagcg cccggcggcg cgttgggatc gccggcgcca tattcgacgg cttcgcgttg
     4021 cgcgtcgttg agcttgacca gccaggcggc ggtatcgaac ggtggcggag attcagcgac
     4081 ggcgagcaca gtggattcgg gcctgagtgg aattgaagcg gcgacgcata ctgtatatcc
     4141 atacaactat cggagacaag cgaccctttt ttctagtcct ttaccgctgg cgtcaggctt
     4201 cgagaggtct gacagaacta tcagcgagac tccacgtcgc atcgtgtcag gcaccctgga
     4261 aatcgcgggc tcgaagccta cctgacgtgc gcgcggcgct tttcgcggcg tagtgcggcg
     4321 cttcgtcggg caacaacgtc gggcgcgcgt tcctccttgg agcggcacca aaaacccggc
     4381 acagcggcgg cgc
//
LOCUS       DATASH010000719         3855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_117370,
            whole genome shotgun sequence.
ACCESSION   DATASH010000719 DATASH010000000
VERSION     DATASH010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117370"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(659..1402)
                     /locus_tag="VHV99_19015"
     CDS             complement(659..1402)
                     /locus_tag="VHV99_19015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiC family protein"
                     /protein_id="HEX3637537.1"
                     /translation="MMFARLPRPPRGLVRGAVYVGALAGLMAMAACSSPPSRFYTLGA
                     DSAGSGAAGSAATVSARTAAAPAWLIEVAPIDVPPQVAKNQLVVQTGPTQVQVLEQER
                     WASLPGDEIRRALSTSLTQQLGTIDVYGTAYPDKTPVYRVSMNVQRFESWPGSHALID
                     AVWSVRAVRSTAVMTCRSVVSEPVSGGYDALVDGHRAALQQVAMQVAVAVKGMAAVSG
                     STRSGSGGAGFAVPACPSSGVSGATSAGS"
     gene            complement(1399..3057)
                     /locus_tag="VHV99_19020"
     CDS             complement(1399..3057)
                     /locus_tag="VHV99_19020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX3637538.1"
                     /translation="MTSPPGPTPASDAPRNDSNGPKLPPQLPDPDIEPRSRWLPSLVW
                     VIPLIAALIGLALVIKSVTEKGPTITITFTSAEGLEPGKTKVKYKDVDVGSVKTITLS
                     KDLSHVLVQVQLTKEGENFAVKDSRFWVVRPRVGASGVSGLTTLLSGAYIGADAGHSP
                     DTEKNFVGLETPPPITGDQKGHQFILHGDSLGSIDIGSPIFYRRVQVGQVVGFSLDKD
                     GTGVTMQVFVSAPFDQYVGTNSRWWHASGVDLRLDSSGLKLNTQSLATVIVGGLSFQS
                     PPGQGVGAQAPNNMTFRLGSDEADAMREPDGVPLRVVMNFNQSLRGLSVGATVDFRGI
                     VLGQVTNIGIDYDPKTRSFTMPVTMNIYPDRLGKRFRETAPVPGSIAGQTMLQQLVKH
                     GLRGQLRTGNLITSQLYVALDIFPKAPPATVDVAGDPLELPTIPNSLDELQVQVADIA
                     KKLDKVPFDQIGSNLNSALKNADQLFTRLDKEVVPEARDTLAAAKQTFGSAEATLQQD
                     SPLQSDVHQALQELTRTLQSLNALSDYLERHPESLLRGKTGDKP"
     gene            complement(3050..3700)
                     /locus_tag="VHV99_19025"
     CDS             complement(3050..3700)
                     /locus_tag="VHV99_19025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEX3637539.1"
                     /translation="MKYVTAKRSGLLSCHACGRVEPRIRSVTPQHCARCGSRLHSRNP
                     DSLMRTWALLIAAALLYIPANLLPVMHTSSLLGSEDDTIMSGVVYFWTSGDWPLAVIV
                     FIASIMVPMLKLSVLVLLTVTAQRRSRWRPDQRTTLYRMVERIGRWSMLDVFVVTLTV
                     ALVRFKSLAVITAGPGAIAFGSVVILTMMASMQFDPRLIWDNVEGSTPKPQDLDHD"
BASE COUNT          800 a         1271 c         1170 g          614 t
ORIGIN      
        1 cactccgcac tgtgtgcgtc gcggatggca tgcatgggaa accggggctt tgcttgtcgc
       61 actgggagcc gtcggcttcg cctcggcgaa atgccgctaa agcgtgagtg cgaaacgtga
      121 tgattggttt tgtgaaatgc gcgtcggcgc gcgcagcgcc gccggaagta tgacggctgt
      181 gtcactggcg ccgaatgtgc gagagcacgg attccagatg caccactacc gtggctgtgc
      241 gggggacccg cttatgagct ggcggtttga gccgctttct tttgcctact tttctttgcg
      301 gcaggcaaag aaaagtaggc gccgccccgc acaggggcga cgctaataga ccaatatcaa
      361 ttcaaggaaa ggccaacacc ataaaagcac agacaaacaa gcgccgcgca ggcaaccaga
      421 ccaaccaaac cccaacaccg taggcaaaca gccaaacaag cgcgaccagc caaacaattc
      481 aaggaaaccc caacgccgca ggcaaacagc caaacaaacg cgaccagcca aacaattcaa
      541 ggaaagccca acgccgcagg caaacagcca aacaaacgcg agcagacaaa caattcaagg
      601 aaagcccaac accgcaggca aacacccaaa caagcgcccc gcaggcaaaa aaaagccatc
      661 aactaccggc agaggtagcc ccactcaccc cactagaagg acacgccggc acggcaaaac
      721 cagctccgcc ggagccactc ctggtagaac cagaaacagc cgccatcccc ttcacagcaa
      781 cagcaacctg catagcaacc tgctgcaacg cagcccgatg cccatcgaca agcgcgtcat
      841 acccgccgct aaccggctca ctgaccacac tccgacaggt catcaccgca gtactccgca
      901 ccgccctcac actccaaacc gcatcgatca acgcatgtga ccctggccat gactcgaacc
      961 gctgcacatt catgctgacc cgatacaccg gcgtcttgtc gggataagcg gtgccataca
     1021 catcgatcgt gccgagctgc tgcgtaaggc tagtcgacaa agcgcgccga atctcatcac
     1081 ccggcaacga cgcccaacgc tcctgctcga gcacctgaac ctgcgtcggt cccgtctgca
     1141 ccaccagctg attcttcgcc acttgcggcg gcacatcgat cggcgcgact tcgatcagcc
     1201 acgccggcgc tgccgcagtc cgcgcactga ccgtggccgc agaaccagca gcaccggaac
     1261 ccgcgctatc ggcgccgagc gtataaaacc ggctcggcgg cgacgaacac gccgccatcg
     1321 ccatcaatcc ggccaaagcg ccgacataca cagcgccgcg cacaagcccg cgcggcgggc
     1381 gggggagccg ggcaaacatc atggtttatc tcctgtttta ccgcgcaaca gcgattccgg
     1441 atgacgctcc agataatccg acagcgcatt cagcgactgc aacgtgcgcg tcaattcctg
     1501 caacgcctga tgaacatccg actgcaacgg cgagtcctgc tgcaacgtcg cctcagccga
     1561 cccgaaggtc tgcttcgccg cagcgagcgt gtcgcgtgcc tccggcacga cttccttgtc
     1621 gagtcgcgtg aatagctggt cggcattctt caacgcgctg ttcaggttcg aaccgatctg
     1681 gtcgaacggc accttgtcga gcttcttcgc gatgtcggca acctgcacct gcagttcgtc
     1741 gagcgagttc ggaatggtcg gcaactcgag cggatcgccc gctacgtcga ctgtggccgg
     1801 gggcgccttc gggaagatat cgagcgccac gtacaactgg ctcgtaatca gattgccggt
     1861 gcgcaactgg ccccgcagac catgcttgac gagctgttgc agcatggttt gacccgcgat
     1921 gctgccaggc accggcgcgg tttcgcggaa gcgcttgccg agacgatccg ggtagatgtt
     1981 catcgtcacc ggcatggtga agctgcgcgt cttcggatcg tagtcgatgc cgatgttggt
     2041 cacctgaccc agcacgatgc cgcggaaatc gaccgtcgcg ccaaccgaca gtccacgcag
     2101 cgactggttg aagttcatca ccacgcgcag cggcacgccg tccggttcgc gcatcgcgtc
     2161 cgcttcatcc gaaccgaggc ggaacgtcat attgttcggc gcctgcgcgc ccacaccctg
     2221 gcccggtggc gactggaacg agaggccgcc gacgatcacc gtcgcgagcg attgcgtgtt
     2281 cagcttgagg ccgctcgagt cgagccgcag atcgacgccg ctcgcatgcc accagcgcga
     2341 attggtgccg acgtactggt cgaacggcgc cgacacgaag acctgcatcg tcacgccggt
     2401 gccgtccttg tcgagcgaga aaccgaccac ctggccgacc tgcacgcgcc ggtagaaaat
     2461 cggcgagccg atgtcgatcg agccgagcga atcgccgtgc aggatgaact ggtggccttt
     2521 ctggtcgccg gtaatgggcg gtggcgtttc aaggcccacg aagttcttct cggtgtccgg
     2581 cgagtgcccg gcgtccgcgc cgatgtaagc acccgaaagc agcgtggtca atccggacac
     2641 gccgctcgcg ccgacgcgcg ggcgcaccac ccagaagcgc gaatccttga ccgcgaaatt
     2701 ttcgccttcc ttggtgagct gcacctgaac cagcacgtgc gacagatcct tcgacagcgt
     2761 gatggtcttc accgagccga cgtcgacgtc cttgtacttg accttggtct tgccgggctc
     2821 gaggccttcg gcactggtga acgtgatcgt gatggtcggg cctttttcgg tcaccgactt
     2881 gatcacgagc gcaaggccga tcagcgcggc gatcagcgga atcacccaga caagggaggg
     2941 cagccagcgg ctgcgcggct cgatatcggg gtctggcagt tggggcggta gcttcggtcc
     3001 gttcgaatcg ttgcgcggcg cgtcggaggc gggcgtcggt cctggcgggc tagtcatggt
     3061 cgaggtcctg aggttttgga gtactgcctt ccacgttgtc ccagatgagc cgtggatcga
     3121 attgcatgga tgccatcatc gtcagaatca ccaccgagcc gaaggcgatt gccccggggc
     3181 cggccgtaat gacggcaagc gatttgaagc gcaccagcgc gacggtcagc gtgacgacga
     3241 acacgtcgag catcgaccag cgcccgatcc gctcgaccat ccggtaaagc gtggtgcgct
     3301 ggtcgggccg ccagcgcgag cggcgctgcg ccgtgacggt gagcagcacc agcacgctca
     3361 gcttgagcat cggcaccatg atgctggcga tgaacacgat caccgcgagc ggccagtcgc
     3421 ccgaggtcca gaaatagacg acgccgctca tgatggtgtc gtcttcggaa ccgagcagcg
     3481 acgaggtgtg catcaccggc agcagattcg cgggaatata cagcagcgcc gcggcgatca
     3541 gcagcgccca ggtgcgcatc aggctgtcgg ggttgcgcga gtgcaggcgc gagccgcacc
     3601 gtgcgcagtg ctgcggcgtg acggagcgga tgcgcggctc gacgcgtccg cacgcatggc
     3661 agctgagcag gcccgagcgt ttcgcggtga cgtatttcat ggcgtgctca tcctcgacgt
     3721 ggcaccggcg gcacggccgg cggcaagtcg gacggcgcgg cgcttcgcac atcgggcgcc
     3781 gccgcgctgt tcacttcggg cggcgcgggc ggcccggcga ccgaactgac cgcggccgcc
     3841 gcgctatcgg cggag
//
LOCUS       DATASH010000720         2525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_314136,
            whole genome shotgun sequence.
ACCESSION   DATASH010000720 DATASH010000000
VERSION     DATASH010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314136"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..2360)
                     /locus_tag="VHV99_19030"
     CDS             complement(24..2360)
                     /locus_tag="VHV99_19030"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018444341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEX3637540.1"
                     /translation="MFRSTTGPSGAGEDRRQLSAREFLDLMRAHVRELAVVTGIVIAL
                     AIGWLFIATPLYSADVMLRVDPPEPNALGIALQNQEAVAPAAPSTAAEITVMQSRSVL
                     QPVIDHYGFNVTVTPRTVPLLGAIAGQFATPGELSRPWLGLDTFAWGGEQLRIGSITV
                     PQNLEEEKLQLTALAGGHYELRGPSGEHLLSGTVGKPASSAGISMTVDRLVARRGTRF
                     EVIRWNSLEAVARFARDVKIAGKGKDNDTGIIVISYMNASPALAAEVANALARQYIAA
                     AVAARQRDDTQTLEFINGELPRLRNELKRTEEALNAFRKSSQSLQPTSEAQTYLQGSI
                     DLQRQIATLQLQRTQLLQRFQPNSEWVRNTDMQLAQLNAAKATFDARFSNMSTSERSN
                     VDLARDARVSEAIYLAMVNKAEQLTVRRASTTAGVNIVDSALRPHQPVKPDRLLVISA
                     GAGIGLFAGAFLVFIRRHVMTGVTDPRYVEHAMGVPVIGEVLFSAQQARLDREISAAT
                     SRAGIVPGSRAAKLPSGHGPSSDTAAGLTRLAQNMPRVLAARFPKDVTVEALRDVRTT
                     LNLGLTHVPGNVVMCTGPTPAAGKSFVAANLAVLQAEIGSRVLLIDADMRRGRLAYFF
                     GQLNRGGLSDVLTGDTEPADVIRSVGVPGLSFLSCGTYPDNPAELLMKRRFKEMLARL
                     GEEYDLVIIDTPPFLSVTDASIVASEAGATVLVLRSGLQTEHEISETLKKLERAEAHL
                     FGAIFNAMPVRSGNRAYHYAMGYKTLELAEEAGESQKA"
BASE COUNT          426 a          828 c          819 g          452 t
ORIGIN      
        1 gtcgcgtgag gctgcgacga aggtcaggcc ttttgcgatt cgccggcctc ctcggcgagc
       61 tccagtgtct tgtagcccat cgcgtagtgg taggcgcgat tgccggagcg caccggcatg
      121 gcgttgaaga tcgcaccgaa caggtgtgcc tcggcccgct ccagcttctt gagcgtttca
      181 ctgatttcat gttccgtctg caggccggag cgaagcacga gaaccgttgc cccggcttca
      241 ctggcaacga tggaagcgtc cgtcaccgag agaaacggcg gcgtgtcgat gatcaccagg
      301 tcgtactcct cgcccaggcg cgcgagcatc tccttgaagc ggcgcttcat cagcaactcg
      361 gcgggattat ccggatacgt gccgcaggac aggaacgaca ggccgggcac gcccacgctg
      421 cgaatcacgt cggccggctc cgtgtcgccg gtgaggacgt cagacaggcc accccgattg
      481 agctggccga agaagtacgc cagacggccg cgccgcatgt cggcatcgat cagcagcacg
      541 cgcgagccga tttcggcctg aagaacggcg aggttcgctg ccacgaaact cttgccggct
      601 gccggcgtcg gccccgtgca catcacgacg ttgcccggca catgcgtgag cccgagattc
      661 agcgtggttc gcacatcgcg tagcgcttcg acggtgacgt ctttgggaaa cctggcggcc
      721 agcacgcgcg gcatgttctg ggcaagccgc gtgaggcccg ctgccgtgtc cgacgacggc
      781 ccgtgcccgg atggcaactt cgccgccctg ctgcccggca cgattcctgc gcggctggtc
      841 gccgccgaga tctcccggtc gagccgcgcc tgttgcgcgc tgaagagcac ttcgccgatc
      901 accggtacgc ccatggcgtg ttcgacatag cgtggatccg tcacgcccgt catcacatgg
      961 cggcgaatga acaccaggaa cgcgcccgcg aacaggccga tgcccgcgcc agccgatatc
     1021 accagcaggc gatccggctt caccggctga tgcggcctga gcgcgctatc gacgatattc
     1081 acgccggcgg tcgtgctcgc gcggcgcacc gtcaactgct cggccttgtt gaccattgcc
     1141 aggtagatcg cctccgagac gcgcgcatcg cgggcgagat ccacgttgct acgctcggac
     1201 gtcgacatgt tgctgaagcg ggcatcgaat gtggcctttg ccgcattcag ctgggccagt
     1261 tgcatgtccg tgttgcgcac ccactcgcta ttgggctgga aacgctgcaa cagttgcgtt
     1321 cgctgcagct ggagcgtggc gatctgccgc tgcagatcga tgctgccctg cagataggtc
     1381 tgggcttcgg aagtcggctg cagcgactgc gatgacttgc ggaacgcatt gagcgcttct
     1441 tccgtgcgct tgagctcgtt acgcaggcgc ggcagttctc cgttgatgaa ctcgagcgtc
     1501 tgcgtgtcgt cgcgctggcg ggccgcgacc gccgctgcaa tgtattgccg cgcgagtgcg
     1561 ttcgccacct cggccgcgag tgccgggctc gcgttcatgt acgagatgac gatgatgccc
     1621 gtgtcgttgt ccttgccctt gccggcgatt ttcacgtcac gcgcgaagcg ggcgacagct
     1681 tcgagcgaat tccagcggat cacctcgaat cgggttccgc ggcgcgcaac cagtctgtcc
     1741 acggtcatcg aaatacccgc cgacgatgcg ggcttgccca ccgtgccgct cagaaggtgt
     1801 tctcccgacg ggccccgcag ttcgtagtgg ccaccggcga gcgcggtgag ttgcagcttc
     1861 tcttcttcaa gattctgcgg caccgtgatc gaaccgatcc tgagctgctc tccgccccac
     1921 gcgaacgtgt cgagtcccag ccacggacgc gacagctcgc cgggcgtcgc gaattgcccg
     1981 gcaatcgcac ccaggagcgg gacggtgcgt ggcgtgaccg tgacgttgaa cccatagtgg
     2041 tcgatcacgg gctgcagcac cgagcggctc tgcatcacgg tgatttccgc ggcggtggag
     2101 ggcgcggccg gcgcgacggc ctcctgattc tgaagcgcga tgccgagcgc attcggttcc
     2161 ggcggatcga cgcgcagcat tacgtcggcg gaataaagcg gcgtggcaat gaacagccag
     2221 ccaatggcga gcgcgatcac aataccggta accacggcaa gttccctcac gtgcgcgcgc
     2281 atgaggtcaa gaaactcccg cgcggacagt tgtctgcggt cctctcctgc gccggaaggt
     2341 ccagtagtgg aacggaacat attcacaggc gtcgtccttg aggtgaaacg cgtattcgac
     2401 cgggtgcccc tgcgcaagcc gaaaggcggc ttccagcccg ggcagagcga gatgtggcag
     2461 gccggcaacc ggctctgctg catgccggat ccgcacacga ccaggcgccc tcattctcgc
     2521 cgcgc
//
LOCUS       DATASH010000721         2860 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5063843,
            whole genome shotgun sequence.
ACCESSION   DATASH010000721 DATASH010000000
VERSION     DATASH010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5063843"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(222..1244)
                     /locus_tag="VHV99_19035"
     CDS             complement(222..1244)
                     /locus_tag="VHV99_19035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEX3637541.1"
                     /translation="MDSVDLEVLKSSARWLEEGHRALLVTVVKTWGSSPRPEGAMLAV
                     RDDGLVVGSVSGGCIEDDLIDRVRQRGIEQTHPEAVKYGITAEEAHRFGLPCGGTIQL
                     VLEPLTPQSRIADLCNAVEDGRLMAREVDMVTGAVRLNAAQATDGVHFDGERLLTIHG
                     PRYRMLVIGAGQLSRYLCQIAVGLDYQVTVCDPREEYTEEWAIPGTKIVRTMPDDTVI
                     EMKLDERCAVIALTHDPKLDDLALMEALKTPAFYVGALGSRRNNQARRERLKEFDLND
                     AELGRLHGPVGIYIGSRTPPEIAVSILAEVTAAKNGVSLPTLLQVEGAKAAREILASG
                     DAACSV"
     gene            complement(1217..1813)
                     /locus_tag="VHV99_19040"
     CDS             complement(1217..1813)
                     /locus_tag="VHV99_19040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HEX3637542.1"
                     /translation="MELSETHRLPVSQQRAWDALNDTGILRACIPGCESIDPVGENAY
                     AVVMSASVGPVKARFKGRMLLTDIEAPNTYTIVFEGQGGAAGFGKGNARVTLEPEGDA
                     ATKLTYTATAQVGGKLAQIGSRLVDGAARKIAGEFFKRFGAQVSGAADGATAPDDGDA
                     AAAEAAGQNTELNEAADTESGGDGTKRKKSWTAWISKS"
     gene            complement(1804..>2860)
                     /locus_tag="VHV99_19045"
     CDS             complement(1804..>2860)
                     /locus_tag="VHV99_19045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX3637543.1"
                     /translation="VCAPDERELFNAAFDLFWRDPDWEGKLRALLLPKVRDGLPPPKR
                     NNRLADALAMRTPPSPHRKAPQQTEQHELHAHVTFSAEERLRHRDFETLSADEWRTLR
                     HLIRGQRLPLATEPTRRLKAASHGTHADLRASARQAVRAGGDWTVWKYRAAVERKPPL
                     VLLLDISGSMSSYSRAVLYFCHALLQSRERLQVFLFGTRLTNATRALRERDPDVAIAA
                     LTDQVVDWSGGTRIGAALAEFNRRWARRVLTGRATVLLVTDGLDHEAIDMLDTEMARL
                     RRFAYRIVWLNPLLRFSGFSPKARGVQAILPYVDAHRPVHNLDSLTAFGRDLAQLTRA
                     PRLTVSARTTAGAIPWN"
BASE COUNT          557 a          972 c          825 g          506 t
ORIGIN      
        1 tgagccgctt tcttttgcct acttttcttt gcggcaggca aagaaaagta ggtgcccgcc
       61 ccgcacaggg gcgacgctaa tagaccgata acaaatcaag gaaaggccaa cgccataaaa
      121 gcacagacaa caagcgccga gcaggcaacc agatcaagaa aaccccaccg ccataggcaa
      181 acaaaccaac caaaaagcgc cgcgcaggca aaaaccacca actaaacact acaagccgca
      241 tccccagaag ccaaaatctc ccgcgcggcc ttagcgccct caacctgtaa taaagtcggc
      301 aacgaaaccc cattcttggc cgccgtcacc tcggcaagaa tcgaaaccgc aatttcgggc
      361 ggcgtccggc tgccaatata aatcccgacc ggcccatgca accggcccaa ctccgcatcg
      421 ttcagatcga actccttcaa ccgctcgcgc cgggcctgat tattccttct cgatcccaaa
      481 gcacccacat aaaaagccgg cgtcttcaac gcctccatca aagccagatc atccagcttg
      541 ggatcgtgcg tcagcgcaat caccgcgcac cgctcgtcga gcttcatttc gatcaccgta
      601 tcgtcgggca tcgtccgcac gatcttcgtg ccgggaatgg cccactcctc ggtgtactcc
      661 tcgcgtggat cgcagaccgt gacctgataa tcgagcccga ccgcgatctg gcacagatag
      721 cgcgacaact gccccgcgcc aatcaccagc atccggtagc gcgggccgtg aatcgtcagc
      781 agacgctcac cgtcgaaatg cacgccgtct gtcgcctgtg cggcattgag ccgcaccgca
      841 cccgtcacca tgtcgacttc gcgtgccatc aaacggccgt cctccaccgc attgcacagg
      901 tcggcaatgc ggctctgcgg tgtcaaaggt tccagcacaa gctggatcgt gccgccgcaa
      961 ggcaagccga agcgatgcgc ttcttccgct gtaatgccgt acttcaccgc ttccgggtgt
     1021 gtctgttcga tacccctttg ccgcacgcga tcgatcaggt cgtcttcgat gcagccaccc
     1081 gataccgaac ccaccacgag cccatcgtca cgcaccgcga gcatcgcgcc ctcgggccgc
     1141 ggcgacgagc cccacgtctt caccaccgtc accagtagcg cgcgatgtcc ctcttccagc
     1201 caacgtgcgc tggacttcag gacttcgaga tccacgctgt ccatgatttt ttcctcttcg
     1261 ttccgtcgcc gccggactca gtgtccgcgg cctcattcaa ttccgtattt tgccccgccg
     1321 cttcggcggc tgcagcgtcg ccgtcatccg gggccgtggc gccgtcagcg gcgccgctga
     1381 cctgcgcgcc gaagcgcttg aaaaattcgc ccgcgatttt gcgcgccgcg ccgtcgacca
     1441 gtcgcgaacc gatctgcgcg agcttgccgc cgacctgtgc ggtcgcggtg taggtgagct
     1501 tcgtggcggc gtcgccctcg ggttcgagcg tgacgcgcgc attgcctttg ccgaagcccg
     1561 ccgcgccacc ctgaccttcg aaaacaatcg tgtaagtgtt cggcgcctcg atgtcggtca
     1621 gcagcatacg ccctttgaag cgcgccttca cgggaccgac cgatgcgctc atcaccaccg
     1681 cgtacgcatt ctcgccaacc gggtcgatgc tctcgcagcc gggaatgcag gcgcgcagaa
     1741 ttccggtgtc gttcagcgca tcccacgcgc gctgctgcga aaccggcaac ctatgggttt
     1801 cactcaattc catggtattg ctcctgcggt agtcctggcg gatacggtga gacgaggcgc
     1861 gcgtgtgagt tgcgcgagat cgcggccgaa cgctgtcaga ctgtccagat tatgaacggg
     1921 acgatgcgcg tcgacatacg gcaggattgc ctgcacgccg cgcgccttcg gagaaaagcc
     1981 gctaaaacgc agcagcggat tgagccacac aatacggtac gcgaagcggc gcaggcgggc
     2041 catttcggtg tcgagcatgt cgatcgcttc atggtcgagg ccgtcggtga cgagcagcac
     2101 cgtggctctg ccggtcagca cgcggcgtgc ccagcgtcgg ttgaattcgg cgagtgccgc
     2161 accgattcgc gtgccacccg accaatcgac gacctgatcg gtcaacgccg cgatggccac
     2221 gtcgggatcg cgctcgcgca gcgcacgcgt tgcattggtg aggcgcgtgc cgaacagaaa
     2281 cacctgcaga cgttcacgcg attgcagcag tgcgtggcag aagtacagca ccgcgcgcga
     2341 atagctgctc atcgaaccgg agatatcgag cagcaagacc agcggcggct tacgttccac
     2401 cgccgcgcga tacttccaca ccgtccagtc gccgcccgcg cgcaccgctt gccgcgcgct
     2461 cgcgcgcaag tccgcgtgcg tgccatgtga ggccgccttc aggcggcgcg tcggttcggt
     2521 ggcaagcggc agacgctggc cgcggatcag atgccgcaac gtgcgccatt catcagcgga
     2581 aagggtttcg aaatcgcgat gacgcagacg ttcttcagca ctaaaggtga cgtgcgcgtg
     2641 caactcgtgt tgttcggttt gctgtggcgc tttgcggtgc ggcgagggcg gggtgcgcat
     2701 ggcaagcgcg tcggccagcc ggttgttgcg tttgggtggc ggcagcccat cgcggacttt
     2761 cggcagcagc agcgcgcgca gcttgccttc ccaatccggg tcgcgccaga acagatcgaa
     2821 ggcggcgttg aagagctcgc gttcgtccgg cgcgcagacc
//
LOCUS       DATASH010000722         7055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4470805,
            whole genome shotgun sequence.
ACCESSION   DATASH010000722 DATASH010000000
VERSION     DATASH010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7055
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4470805"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="VHV99_19050"
     CDS             <1..391
                     /locus_tag="VHV99_19050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637544.1"
                     /translation="RHLDQLDTLITLASSICRNPSITVDERRRRQTLLELLVDTMEQY
                     QQELECDRELYQVIALDAKGVAQGRITARQAANLLAEASQRAKEPVAGANAPECKSTS
                     SEGAATLKGGAIQRPVVAARQMIRKGL"
     gene            1069..2061
                     /locus_tag="VHV99_19055"
     CDS             1069..2061
                     /locus_tag="VHV99_19055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637545.1"
                     /translation="MLAAAALSGCASAPGATEASNASSAAAAQGDPSRDALNMVARQW
                     SDPAFFPGNRPLGFWFVATYTDKWRITATSEVPDNLQHTQYDQDIFYYSFDANTIQPA
                     YPQFDVHDTTTYFSCPADTRPPVGAPCASPVVDSSNTVRALGVLFGHQDVVNNKPSFF
                     SANKIAQMIDGIGVVQLRSVANQMHMDRYNREYGRAQNNREDAETFIRDFKRWDPMNR
                     VATIEADLPALRRKEAAEQTAHARDAAKAENDRREKIFRTARRGSQLFCHTGNFVLGE
                     NAGLDALAYDCSGIGIISLSELQSHGYVVTSQDQVAVPTYSGRGYRVSLILTKQ"
     gene            2488..4065
                     /locus_tag="VHV99_19060"
     CDS             2488..4065
                     /locus_tag="VHV99_19060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637546.1"
                     /translation="MSGASAGTSANGHPGTSSTTGNSGGTNAAGTVTLGGTAYDSAIP
                     GAIITVTSGAPLGQSGATTIATALANASGAYTATAPIPSGNTAVFINAADPRNANNVF
                     TGYLGQSTDLVSGTTLDDNLLPNLDVGPVTTAAVALYASLHGNSYAGLTRASYTQALT
                     PNTADVSIVAAGLKAVGDALCQPAASHAIANLATVLAAGTSLGSGLYSTALEETTFVL
                     GGNCGPVVETLMQAVSADPIYGPQLAGWSGPNSTMESIAGAYEMQGVIAESGLVGGTL
                     SAQSLAAAPTSTPASVFSDTITIAGGTGSVTSADGRVEGTMNGNVLNLSVKNGGQTYT
                     VRMPIIRLTSALSSSGGYALGGSGQNSSTSVLTNFPAVLVPVGASPTWTGLASNLVQP
                     AASTAMGVTCESGLPMLLHGYVNGVGGGSVGECVSPNATGWTMDLPTAIAQPFGFNYA
                     TGRSSATEPVLATPSWTQTESAPFVLTSTASYALSSSSSPTTSGQMYAVANSPVLIFT
                     TASGNNVFRAVNNPLTW"
     gene            4278..4739
                     /locus_tag="VHV99_19065"
     CDS             4278..4739
                     /locus_tag="VHV99_19065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637547.1"
                     /translation="MAPAKTIALPNAQVASIDQVSMCAARRRLLFAGAAALMPGALRA
                     QSAHSVEDSAEPFGGPPEVKRQLASAVAQASAQLKFHRPFEAAQILAAASTLIEAAPA
                     TRWPWRALVDPLNGYALLSLGQNQQALDVLQRGWTPSSASSANRSSCLQRC"
     gene            4733..5383
                     /locus_tag="VHV99_19070"
     CDS             4733..5383
                     /locus_tag="VHV99_19070"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEX3637548.1"
                     /translation="MLTQHELHLGFAFFRQEFVRQVFAALVVEGRDQPHTIEELIFDE
                     SAGSAEIRFTLARAMSACARNDAIAKLYRDHVSEERLADDPYVHIAHAASARGDTRRA
                     SRAGLHCRRPELFAAARPGRQGGRAERERMVGFYRALAQSLPKSEALRQAKLALRDAG
                     YPPSAWAGFLFAGSDAALSVWGELASVSTAQAAFGRTQERGTVKLGKRAQRCARCA"
     gene            complement(5359..5889)
                     /locus_tag="VHV99_19075"
     CDS             complement(5359..5889)
                     /locus_tag="VHV99_19075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637549.1"
                     /translation="MAGRHAFFRALPAGLLAASLAAFSTHAFAGASDTAGDPFGEAGS
                     PELRVLQYEAVLGDPLDARNGACVDDAMGHRLDIPMGDDGSAQRAADEVRAAAEQCSL
                     AQGGDHLRLVGGVRKALADQMFLRASSLAGARSCLDKAPSLNALRDCVTAAIGVPPSA
                     AEWPRWTALYAQRAQR"
     gene            complement(5889..>7055)
                     /locus_tag="VHV99_19080"
     CDS             complement(5889..>7055)
                     /locus_tag="VHV99_19080"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX3637550.1"
                     /translation="GLAALEWWSTLSQGIGIGATGVFSGIAALALMFVVSLALGRGHD
                     EGHSQWAQYMSATLLGITELIVGVGIAGALATLVIGLLPYEVDVRLFDNDRMTDVFLF
                     SIIGVGVLVSARSWRRYAQQKGRTMQAQITAAETRTALEEQKSRALQARVEAAEARAA
                     LEEERLRTQVERAQDRAALLEKEKDLANAQLALLRKQIEPHFIFNTLGSVQRLVEKDP
                     QAAARMVEHLTLYLRTTLYETKGHLSSLRSEFESARAYLEIMKQRMGERLSVSLDLPD
                     ALATLTFEPLLVHTLVENAIKHGVEPKIGPVSISLSARIDEGEPRRLIVEAKDTGVGL
                     QSNAATSGTRLGLDNVRKRIKARYGDDARLSIEAAPEGGVVSRIALPLASMRTS"
BASE COUNT         1347 a         2253 c         2225 g         1230 t
ORIGIN      
        1 ccggcacctg gatcaactgg acaccttgat cacacttgcg tcctcgatat gtaggaatcc
       61 ttctatcacg gtcgatgaaa gacggcgtcg tcagacgctg cttgaattgc tggtggatac
      121 gatggagcag tatcagcagg aactcgaatg cgaccgcgag ttgtatcagg tgatcgcgct
      181 tgatgcgaag ggcgtggcgc aaggccgcat cacggcgcgg caggcggcga acctgctggc
      241 tgaggcgtcg cagagagcga aagaaccggt agccggcgcg aatgctccag agtgcaaaag
      301 cacgtcatcg gaaggcgccg caacgttaaa aggcggtgcc atccagagac cggttgtcgc
      361 ggcacgccag atgatcagga aggggctctg aggggcagag ccgtgcatcc gaatcgttcg
      421 gagattcatg cactgagacg gctttcgtcc gtctcagtgc gggccacaaa cggtcagtca
      481 tgcgggtctc gtaaatcgtc ggggatcggg gtcggcaagc ctctgattgg ggcgcgacgt
      541 cggcgctagc atattttccc gtccaaaaac ttttgccgcc gtagccacca ggtgagaatc
      601 gcagcaaggc gatatggcgt acgcaggccg gcctttctca agacaatccc ggatgctcgc
      661 atactccacg tcatggaacg aatcgcgcga tcagcagcat gggcttgaaa gcctgcggcc
      721 aacgcatgac ctcacaacac gccctcggat tcaaaacgac cgccatctcc tggatgcgtt
      781 ttccagcatg ccctcacaga cgaacgtatc gtcgccgggg cttggggcat ggtggcactg
      841 tctaaagaat tcccgtttcc ggccgatatg accgcgtggg cgcagtaatc cgggatcggt
      901 gcactgtcct cgaaaatgaa gtcaatcttt acgcgaaacg gagaggaaat gaagcggttg
      961 cttgcaggac tgccggatat ggtccctggc actcggtgtg ctggggttgg cggaagtatt
     1021 aaagacactt gggcgcgcaa ggggaagtcc gcgatttgcg gggtcgttgt gctggcggct
     1081 gcggccctga gcggctgcgc ttcggcaccg ggcgccaccg aggcgagcaa tgcgagcagc
     1141 gcggcggccg cgcaaggcga tccgtccagg gacgcactga acatggttgc gcgccaatgg
     1201 agcgatccgg cattcttccc aggaaatcgt cctttgggat tctggttcgt cgcgacatac
     1261 acggataaat ggcgcatcac tgcaacgagc gaagtccccg ataacctgca gcatacccag
     1321 tatgaccagg acatctttta ctacagcttc gacgcaaata cgatccagcc ggcttacccg
     1381 cagttcgacg tccacgacac cacgacgtat ttctcctgcc ccgccgacac ccggcctcca
     1441 gtaggggcgc cgtgcgcgag tcccgtggtc gatagcagca acaccgttcg ggcgttgggc
     1501 gtgcttttcg gccatcagga cgtcgtgaac aacaagccca gcttcttcag tgcgaacaag
     1561 atcgcgcaaa tgatcgacgg gatcggtgtg gtgcaactac gaagcgtcgc aaaccagatg
     1621 cacatggatc gctacaaccg cgaatacgga cgggcgcaga acaaccggga agatgccgaa
     1681 acatttatcc gcgacttcaa gcgttgggac ccgatgaacc gggttgccac tatcgaggct
     1741 gacctgccgg cgttgaggcg caaggaggcg gcggagcaaa ccgcacacgc acgcgatgcc
     1801 gcaaaggctg aaaacgacag acgcgaaaag atcttccgca ccgcccgacg cggcagtcag
     1861 ttgttttgcc acacgggcaa tttcgtactt ggcgaaaacg cagggctcga cgcactcgcg
     1921 tacgactgtt ccgggattgg aatcatcagc ctgagcgagc tgcaatcgca cggctatgtt
     1981 gtgacgtctc aagaccaggt tgcggttccg acttattcgg gccggggtta tcgcgtctcg
     2041 ctgatcctga cgaagcagtg agctccggtt gcggccgtgc cgattccttt tgagggcgac
     2101 cgcacgcatg tcccgctgcg acacgagtcg tggcggttca tgcctccgcg ttcgtcgcgc
     2161 tcagggagcg ttcgtcgcca gcgcccggcg ttagtcgcat cggcaatgct caagcacaag
     2221 cggaccaata cagtcccgat tgcgtcgcag cggtgccgtg ttcgatcagc ttcgtacccg
     2281 gtcatcgcga cgatccatat ccgaatgccg gaagcccggc gcttgaagca ggtatttccc
     2341 gaggagcagg agatgaagaa gaaccgaacg tgcccggctt ttattaatcc cggtctgtgg
     2401 tcaacgcgat ccctgatgtt gccgctggcc gtgggtttcg caatcactct cagcgcatgc
     2461 ggaggcgcag gcaatggcgg tgcagacttg tccggcgcat cggccggcac gagcgcgaac
     2521 ggacaccccg gcaccagttc gacaaccggc aactccggcg gcacgaatgc cgcaggcacg
     2581 gtaacgctcg gcggaacggc gtacgactcg gcgattccgg gcgcaatcat taccgtcacg
     2641 tccggtgctc ctctcggtca gtccggcgcc acgacgatcg caaccgcgct cgccaacgca
     2701 agcggcgcgt acacggcgac tgcgccgatc ccttcgggta acacggcggt cttcatcaac
     2761 gccgctgacc cgcgcaatgc caataacgta ttcaccggct atctgggcca atcgacggat
     2821 ctcgtctccg gcaccacgct ggatgacaac ctactcccca acctcgacgt cggtcccgtc
     2881 accacagccg cagtcgcgct atatgcaagc ctgcatggca actcgtatgc aggtctcacg
     2941 cgtgcttcct atacacaggc actgacgccg aacaccgccg acgtctccat cgtggccgcg
     3001 ggtctgaagg cagtcggcga cgcgctttgc cagcccgccg cgagtcacgc catcgcgaat
     3061 ctcgcgaccg tattggcggc tggaacgagt ctgggttcgg ggctgtattc gacggcgctg
     3121 gaggaaacca cgttcgttct cggcggaaat tgcgggcctg tcgtcgagac gctcatgcag
     3181 gcggtgtcgg ccgatccgat ctacggcccc caactggctg ggtggagcgg ccccaacagc
     3241 accatggagt cgatcgccgg agcgtatgag atgcaggggg tgatcgcgga gtccggcctc
     3301 gttgggggaa cgctctccgc acagagcctg gccgccgcac ccacgtcgac gcccgcgagc
     3361 gtcttctccg acacgatcac gattgcgggc gggacgggaa gcgtcacttc cgccgacggc
     3421 cgcgtcgagg gaacgatgaa tggcaacgtg ctgaacctga gcgtgaagaa tggtggccaa
     3481 acgtacaccg ttcgcatgcc gatcatccgt ctgacgtcgg ctctgagcag ttcgggaggg
     3541 tacgcgctag gcggcagcgg gcaaaacagt tcgacttccg ttctgacgaa tttccctgcg
     3601 gtacttgtgc ccgtgggcgc gtcgcctaca tggacggggc ttgcttccaa cctcgtgcag
     3661 cccgcggctt ccacggcgat gggcgtgacg tgtgagagcg ggctgccgat gctgttgcac
     3721 gggtacgtca acggcgtggg cggcggttcg gtcggcgaat gcgtatcgcc gaacgccacc
     3781 ggctggacga tggacctgcc caccgcgatt gcacagccct tcggttttaa ctatgcgacg
     3841 ggtcggagct ccgcgacgga accggtgctt gccacgccga gctggacgca aaccgaatcc
     3901 gcgccgttcg tgctgacgtc cactgcgagc tatgcgctca gttcaagctc gtcgccaaca
     3961 accagcggac agatgtatgc ggtcgcgaat tcgccggtac tgatcttcac gacggcgagc
     4021 ggcaacaacg tgttccgtgc ggtgaataat ccgctgacct ggtaagaggg acgtccacgc
     4081 gttccggtat cgccacagag attgtctgcg cgcagcggag gcaatccctg atcggtttgg
     4141 agcgcaccag gacgatacgt ttgcgcagcg cgcggctaaa gaaacaccgc ctagcggccg
     4201 ttagccgaag gccgagtgcc gtcgccaacg cgacagatct ccggcactga tctcttcgtc
     4261 acggcaatcc acaagccatg gcgcccgcca agaccatcgc acttcccaac gcccaggtgg
     4321 cgtcgatcga ccaggtcagc atgtgtgcag cgcgccgccg cttgctgttc gccggagctg
     4381 ccgcgctgat gccgggcgcg ctgcgcgcgc agagcgccca tagcgtcgaa gacagcgcgg
     4441 agccgttcgg cggcccacct gaagtgaagc gacagcttgc atcggccgtc gcgcaggcat
     4501 ccgcccagtt gaaatttcac cgcccattcg aagcggcgca aatcctggcc gccgcctcga
     4561 cgcttatcga agccgcgcct gcgacgcgtt ggccgtggcg tgcgctcgtc gatccgctca
     4621 acggatatgc gctgctcagt ctcggacaaa accagcaggc gctcgacgtt ctgcaacgcg
     4681 gctggacgcc gagcagcgca tcctcagcga acaggtcgag ctgtttgcaa aggtgctgac
     4741 ccaacacgag ctgcacctcg gttttgcgtt cttcaggcag gagttcgttc ggcaggtatt
     4801 cgcggcgctg gtggtcgaag gacgtgacca gccgcacacg atcgaagaac tcatctttga
     4861 cgaaagcgct ggcagcgccg aaattcgttt cacactcgcg cgggcgatgt cggcgtgcgc
     4921 ccggaacgat gcgatcgcaa agctctatcg cgatcatgtg agcgaagagc gcctggccga
     4981 cgacccctac gtgcacatcg ctcacgcagc ttctgcgcga ggcgacaccc gccgtgcaag
     5041 ccgggccggc ctgcattgtc gccgaccaga gctattcgcc gccgctcgcc caggacgaca
     5101 aggcggacgg gcagagcgcg agcggatggt gggtttctat cgcgcgctcg cacagagtct
     5161 gcccaagagt gaagcgctga ggcaggccaa gctcgcgctg cgcgacgcgg gctatccgcc
     5221 gtcggcgtgg gccggctttc tgtttgcggg cagcgacgcc gcgctcagcg tgtggggcga
     5281 gctggcgtcc gtcagcacgg cgcaggcggc cttcgggaga acgcaagagc gggggacagt
     5341 gaagctggga aagcgcgctc agcgctgcgc gcgttgcgca tagagcgcgg tccagcgcgg
     5401 ccattccgcc gccgacggcg gaacgccgat cgcggcggtc acgcaatcgc gcaacgcgtt
     5461 gaggctcggc gccttatcga gacagctgcg cgctcctgca agcgacgacg cgcgcaggaa
     5521 catctgatcg gcgagcgcct tgcgcacgcc gcccacgagc cgcagatgat cgccgccctg
     5581 ggcgagcgag cactgctcgg cggccgcacg aacctcgtcc gccgcgcgct gcgcactacc
     5641 gtcgtcgccc atcggtatgt cgagacgatg tcccatcgcg tcgtccacgc atgcgccgtt
     5701 ccgcgcgtcg agaggatcgc cgagcaccgc ttcgtactgc aggacgcgca gctcgggcga
     5761 gccggcttcg ccgaacgggt cgccagccgt gtcgctcgcg ccagcgaaag cgtgagtgct
     5821 gaatgcggcg agcgatgcag ccagcaaccc ggccggcagc gcgcgaaaaa aagcatggcg
     5881 gccagccatc acgatgtcct catactcgcc agcggcaacg caattcgtga aacgacgccg
     5941 ccttcgggtg cggcctcgat ggacagccgc gcgtcgtccc cgtaacgcgc cttgatgcgc
     6001 ttgcgcacgt tatcgagccc gagtctcgtg ccgctggtcg ccgcattgct ttgcaggccg
     6061 acgccagtgt cctttgcctc gacaatcagc cgccgcggct cgccttcgtc gatgcgcgcg
     6121 ctcaatgaaa tcgaaaccgg cccgatcttg ggttcgacgc catgcttgat ggcattttcg
     6181 acgagcgtat gcaccagcag cggttcgaag gtgagcgtag cgagcgcatc cggcagatcg
     6241 aggctgacgc tcagacgctc gcccatgcgc tgcttcatga tctccagata ggcgcgcgcc
     6301 gattcgaact ccgagcgcag cgacgagaga tggcccttcg tttcgtaaag cgtcgtcctg
     6361 agatacagcg tgaggtgctc caccatccgc gccgccgcct gcgggtcttt ctcgaccaga
     6421 cgctggacgc tgccaagcgt gttgaaaatg aaatgcggct cgatctgctt gcgtagcagg
     6481 gccaactggg cgtttgccag atccttttcc ttttccagca gcgcggcgcg gtcctgagca
     6541 cgctccacct gtgtgcgcag acgttcctct tcgagcgccg cacgtgcctc ggccgcttcg
     6601 actcttgcct gcaaggcacg ggatttctgt tcctccaacg cggtgcgcgt ctcggcggcc
     6661 gtgatctgcg cctgcatcgt gcgccccttc tgctgtgcat agcgccgcca gctgcgcgcc
     6721 gacacgagca cgccgacgcc gatgatgctg aacaggaaaa cgtcggtcat gcggtcgttg
     6781 tcgaagaggc gcacgtcgac ctcgtaaggc aacaggccta tcaccagcgt cgccagcgcg
     6841 ccggcgatac ccacgccgac gatcaactca gtgatgccca gtagcgtcgc gctcatgtac
     6901 tgcgcccact ggctgtggcc ttcgtcgtga ccgcgtccga gcgcaagcga gacgacaaac
     6961 atcagcgcga gcgccgcgat accggaaaat acgccagtcg cgccgatgcc gattccttgc
     7021 gaaagcgtcg accaccattc gagcgcggcc agccc
//
LOCUS       DATASH010000723         7766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_314184,
            whole genome shotgun sequence.
ACCESSION   DATASH010000723 DATASH010000000
VERSION     DATASH010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7766
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314184"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..97)
                     /locus_tag="VHV99_19085"
     CDS             complement(<1..97)
                     /locus_tag="VHV99_19085"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591239.1"
                     /note="catalyzes the hydrolysis of acylphosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEX3637551.1"
                     /translation="MAPDLDLRIETYYVRVRGTVQGVGFRHATVRQ"
     gene            468..1484
                     /gene="hpnA"
                     /locus_tag="VHV99_19090"
     CDS             468..1484
                     /gene="hpnA"
                     /locus_tag="VHV99_19090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid-associated sugar epimerase"
                     /protein_id="HEX3637552.1"
                     /translation="MTEQNRDRDLVLVTGASGFVGSSVARIAQQKGFKVRVLVRATSP
                     RKNVEALDAEIVVGDMRDEASMRNALRGVRYLLHVAADYRLWAPDPSEIERSNLEGTE
                     ATMRAALKEGVERIVYTSSVATLKVTSSGQSADETSPLKADQAIGVYKRSKVLAERAV
                     ERMIAEDGLPAVIVNPSTPIGPRDVKPTPTGRIIVEAALGKIPAFVDTGLNLVHVDDV
                     AAGHFLALERGKIGERYVLGGENLPLQQMLADIAALTGRKAPTLSLPRGPLYPLAIGA
                     EAVAKFTKREPFLTVDALRMSKNKMYFTSAKAERELGYRARPYREGLSDALEWFRQAG
                     YLKP"
     gene            1713..2288
                     /locus_tag="VHV99_19095"
     CDS             1713..2288
                     /locus_tag="VHV99_19095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637553.1"
                     /translation="MNLHEQLGALEVGVDQLIQAVVAPQAENIPETPAEAAQAASAAN
                     HVSAESKAEDLAPEPAAATTSVEAAASAPAPSSEEPPVQPQPTLEISRPAQNEITMTI
                     GGQTVALRPEQIGQLIEELANARASMTPEPPPGISPGWRFVSTRNPVMAVQKQSNGDR
                     LLVMRHTGHGWVPFTFSPDMVIQMYMMLTKG"
     gene            complement(2374..3546)
                     /locus_tag="VHV99_19100"
     CDS             complement(2374..3546)
                     /locus_tag="VHV99_19100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3637554.1"
                     /translation="MAAVLFLLSSLSLLIWGVLLLARGGFWRARPAAPLTLEPRESWP
                     AVAAVVPARNEVDVIAQAVTTLLEQDYAGEFHVIVVDDHSTDGTADAARAAALQLQCP
                     DRLTVLSAKPLPPGWSGKVWAQSQGIEAVRTLGLPADFLLLTDADIGHPADAVAQLVA
                     RADAEQRDLVSLMVRLRCDSFWEKALIPAFVFFFAKLYPFAWVNNPRNRTAAAAGGCM
                     LVRRSALEEAGGIESIRAELIDDCSLAARIKHRGTGRHPIRLDVAARSVSLRPYDSWR
                     DIWNMIARTAFTQLHYSPLLLAGTLLGMTIIYLMPPVAALVLGPLGWPAWLAWALMCC
                     AYAPMLSYYRRSPLWAPFLPLVALFYVGATFASAVRYWRGKGGQWKARVQAPVQER"
     gene            complement(3558..4424)
                     /gene="hpnC"
                     /locus_tag="VHV99_19105"
     CDS             complement(3558..4424)
                     /gene="hpnC"
                     /locus_tag="VHV99_19105"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HEX3637555.1"
                     /translation="MDVDQYENFPVARVLLPKTLRAPIEIIYHVVRTAADIADEGDWS
                     PADRHARLADFRAGLDAIAQRRAAPVHPLLFGKLAGVIAQYKLPLAPFYDLLHACGQD
                     IDTTRYADRAALLDYCRHSASPIGHLMLHLVGAATPQNLADADAICTASQLISFWQDV
                     AADWKKDRLYLPLADLRRFNVTEAQIARGVVDEAWTKLMAYEISFVRATLVRGAPLAL
                     RIPGRLGIELCGAIHGGLRILERIEKAGYDVFRERPVLNTFDWGVVAVRTVVMRLSRR
                     VGVEAGSLEGNA"
     gene            4757..5701
                     /gene="ispH"
                     /locus_tag="VHV99_19110"
                     /gene_synonym="lytB"
     CDS             4757..5701
                     /gene="ispH"
                     /locus_tag="VHV99_19110"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX3637556.1"
                     /translation="MRVILAQPRGFCAGVVRAIEIVDRALQQHGAPVYVRHEIVHNRH
                     VVDNLRQKGARFVEELDEVPHGAVAIFSAHGVAQTVERDAAQRGLDVLDATCPLVTKV
                     HVQGRQYVAAGRMLILIGHAGHPEVEGTIGQIPGKVRLVQSEAEVAHLDLPHDTPLAY
                     VTQTTLSVDDTRGIIDALLRRFTDIVGPDTRDICYATQNRQAAVRELSKEVDVLLVVG
                     ATNSSNSNRLREIGSETGVASYLVADGSEVKPEWFANVQTVGITAGASAPEEMVKNVI
                     DALRALGPVDVTTMAGREEKVEFKLPSKLMQPLAAREV"
     gene            5712..6872
                     /gene="hpnH"
                     /locus_tag="VHV99_19115"
     CDS             5712..6872
                     /gene="hpnH"
                     /locus_tag="VHV99_19115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HEX3637557.1"
                     /translation="MSIPLLQKVRVGAYIMRQHLSGNKRYPLALMLEPLFRCNLACNG
                     CGKIDYPDPILNQRLSLEECLGAVDECGAPVVSIAGGEPLLHKEMPQIVKGIIARKKF
                     VYLCTNALLMEKKMDDYEPNPYFVWSVHLDGDEQAHDHSVSQEGVYVKAVAAIKEAKR
                     RGFRVNINCTLFNDAVPERVAAFFDTLGPMGVDGITVSPGYAYERAPDQQHFLNRDKT
                     KHLFREIFKRGNNGKNWSFSQSGMFLDFLAGNQTYECTPWGNPARTVFGWQKPCYLVG
                     EGYVKTFKELMETTEWEKYGTGNYEKCADCMVHCGFEATAVMDTVAHPLKALKVSLRG
                     PKTSGAFVKDIALDKQRPAEYVFSRHVEIKLEEIGRTGKGKKVQTAASASAH"
     gene            complement(6993..7598)
                     /locus_tag="VHV99_19120"
     CDS             complement(6993..7598)
                     /locus_tag="VHV99_19120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3637558.1"
                     /translation="MKRYLSAFLAAAVVSTAAYAQSAPDALVKSAVEGTVAAMKADPQ
                     ARGGDMAKITELVQTRFVPATDFQRTTRIAVGKAWSTATPEQQKQLVEQFTLLLVRTY
                     AASLSQLRDQDVTFKFLPVTVPAGANDLVVQSHVISNGGDDAIGYRLTKGANGWKIYD
                     INMMGAWLIQVYQTQFAEQLSKGGVDGLIKFLTAHNARSAG"
BASE COUNT         1563 a         2476 c         2418 g         1309 t
ORIGIN      
        1 cctgccgcac tgtggcgtgg cgaaagccca cgccctgcac cgtgccgcgt acgcgcacgt
       61 aatacgtttc gatccgtaga tccagatccg gagccatgcc gtcctctctt ctgtcgaccg
      121 gagttggcgc tttagcgctt attccggtcc catgcaccgc tgtcgacccg gagttagcgc
      181 ttcagctgtc gatcagagtt agcgcttcag cgcctactct ggccccatgc aatgcactta
      241 ctccgatccc atgcaacgct gtcgacccgg aggtagcgct tcagcactta ctccggtccc
      301 atgcaacgca aaatctttca agccggcatt ctagtcgcgt cgcggcaaga ttgctcagca
      361 gcgcatgagc tcgtccgagc tcgctcacgc gctcaaggcg cccggcgcaa cacacggcgc
      421 gcgcgccacg tacaatccgt cgactgtcac gcagggaatg aatcagaatg accgaacaga
      481 atcgcgatcg cgatctcgtg ctcgtgaccg gcgcttccgg cttcgtcggc tcgtcggtgg
      541 cgcgcatcgc gcaacagaag ggtttcaagg tgcgcgtgct ggtgcgcgcc accagtccgc
      601 gcaaaaacgt cgaggcgctg gatgcggaaa tcgtcgtcgg cgatatgcgc gacgaagcgt
      661 cgatgcgcaa cgcgctgcgc ggcgtgcgct atttgctgca cgtggcggca gactatcgcc
      721 tgtgggcgcc ggacccgagc gaaatcgagc gctcgaatct cgaaggcacc gaggcgacca
      781 tgcgcgcggc gttgaaggaa ggggtcgaac gcatcgtcta cacgagcagc gtcgccacgc
      841 tgaaggtcac cagttccggc cagtccgccg atgagacctc gccgctcaaa gccgaccagg
      901 cgatcggcgt atacaagcgc agcaaggtgc tggccgagcg cgcggtggag cggatgatcg
      961 ccgaggacgg cctgcctgcc gtgatcgtca atccgtccac accgatcggt ccgcgcgacg
     1021 tcaagccgac gccaaccggc cgcatcatcg tcgaggcggc gctcggcaag attcccgcgt
     1081 tcgtggatac ggggctgaac ctcgtgcacg tggacgatgt ggccgccggg cattttctcg
     1141 cgctcgaacg cggcaagatc ggcgagcgct acgttctcgg cggcgaaaat ctgccgctgc
     1201 aacaaatgct cgcggatatc gccgcgctga ccggccgcaa ggctccgacg ctgagtttgc
     1261 cgcgtgggcc gctttacccg ctggccattg gcgccgaagc ggtcgcgaag ttcacgaagc
     1321 gcgaaccgtt cctcactgtc gacgctttga gaatgtcgaa gaacaagatg tacttcacat
     1381 cggccaaggc ggaacgcgag ctcggttatc gcgcgcggcc ttaccgcgag ggcttgagcg
     1441 atgcgctcga atggttccgg caagccgggt atttgaagcc ctgatgcgcg catgacaatg
     1501 gggcggggtg cgtgagtcgg gctgcatggg ccgggttgta taggccaagc cgcgtaggcc
     1561 gggaccgcaa gcggcccgcg taggccgcgc aggtcgcgcc gaacgccccc ggcgcgacct
     1621 gcacgcactt tgttacaacg tctccgtgtt tgcggcagat aaaagcccgc gcgtagcagg
     1681 taaaatcgcg ggtcttacca gagaaacctc gcatgaatct tcacgaacag ctcggcgcgc
     1741 tggaagtggg cgtcgatcag ctcatccagg ctgtcgtggc cccgcaggcc gaaaacatcc
     1801 ccgagacacc cgcagaggca gcgcaggcag cgtcggcggc gaatcacgtc tcggccgaat
     1861 cgaaagcaga agacctggcg cctgaaccag cggcagccac gacctcggta gaagcggcgg
     1921 cgtcggcacc tgcacccagc agcgaagaac cgcccgtcca gccgcagccg acgctcgaaa
     1981 tcagccgccc tgcgcaaaac gagatcacga tgacgatcgg cggccagacg gtcgcgctgc
     2041 gtcctgagca gatcggccaa ctgatcgagg aattggcgaa cgcgcgggcc tcaatgaccc
     2101 cggaaccgcc gccgggcatc tcgccgggct ggcgcttcgt ctcgaccagg aatcccgtca
     2161 tggccgtgca gaaacaatcg aatggcgacc gcctgctggt catgcgccac accggccacg
     2221 gctgggtgcc gttcacgttt tcgcccgaca tggtgatcca gatgtacatg atgctcacaa
     2281 agggctgaca tacggtcgag gtaaaggtcg aggtaaaaaa acggcgcccg agagcgccgt
     2341 ttttttcgtc cgacaaatcc gtgagcgcgt gtttcagcgt tcctgcaccg gcgcctgcac
     2401 acgcgccttc cattgcccac ccttgccccg ccagtagcgc accgccgacg cgaacgtcgc
     2461 gccgacatag aacaacgcca ccagcggcaa aaacggggcc cagagcggcg agcggcggta
     2521 gtagctgagc atcggcgcat aagcgcagca catgagcgcc catgcgagcc acgcgggcca
     2581 gcccagcgga ccgagcacca gcgcggccac cggcggcatc agatagatga tcgtcatgcc
     2641 cagcagcgtg cctgccagca ggagtggcga gtaatgcagc tgcgtaaagg ccgtgcgggc
     2701 gatcatgttc cagatgtcgc gccagctatc gtaaggacgc aacgacacgc tgcgcgccgc
     2761 cacatccaga cggatcggat gacgccccgt gccacgatgc ttgatgcgcg cggcgaggct
     2821 gcaatcgtcg atcagctcgg cacggatcga ctcgatgccg cccgcctctt ccagcgcgct
     2881 gcgccgcacc agcatgcagc cacccgccgc cgcggccgtg cggttacgcg ggttgttgac
     2941 ccacgcgaac ggatacagct tggcgaagaa gaacacgaag gcgggaatca gcgccttctc
     3001 ccagaacgaa tcgcagcgca agcggaccat cagcgagacg aggtcgcgct gttccgcatc
     3061 cgcccgggcg accagttgcg cgacggcgtc ggcggggtgg ccgatgtcgg cgtcggtcag
     3121 cagcagaaaa tcggcaggca ggccgagcgt gcgcaccgcc tcgatacctt gcgactgcgc
     3181 ccacaccttg ccggaccagc ccggcggcag cggcttcgcg ctcaacaccg tcaggcgatc
     3241 cgggcattgc agttgcaacg cggccgcacg agcggcgtcg gcggtgccgt cggtgctgtg
     3301 gtcgtcgacg acgatgacgt ggaattcgcc cgcatagtcc tgctcgagca aggtggtgac
     3361 cgcttgcgcg atcacatcga cttcattgcg cgccggcacc acggcggcaa ccgccggcca
     3421 gctttcacgc ggttcgagcg taagcggcgc ggcgggacgc gcccgccaga aaccgccccg
     3481 cgccaacagc aacacacccc agatcagcag cgaaagagac gaaagaagaa atagaaccgc
     3541 cgccattaag cccgccatca agcattaccc tcgagtgaac ccgcttccac gccgacgcgc
     3601 cgcgacaggc gcatcaccac agtgcgcacc gcgaccacgc cccagtcaaa cgtgttcagc
     3661 accggccgct cgcgaaacac gtcatagccg gccttttcga tccgctcgag gatgcgcagg
     3721 ccaccgtgga tggcgccaca cagctcgatc cccagtcgcc cgggaatgcg caacgcgagc
     3781 ggcgcgccgc gtaccagcgt ggcccgcaca aacgaaattt cgtaagccat cagcttcgtc
     3841 catgcctcgt cgacgacgcc cctggcgatc tgcgcctcgg tcacgttgaa gcgccgcaga
     3901 tccgccaagg gcagatacag gcggtccttt ttccagtcgg cggccacgtc ctgccagaag
     3961 ctgatcagtt gcgaggccgt gcagatcgca tcggcgtcgg cgagattctg cggcgtggcg
     4021 gcgccgacca gatgcagcat cagatgtccg atcgggctcg ccgagtggcg gcaatagtcg
     4081 agcagcgcgg cccggtccgc gtaacgggtg gtgtcgatgt cctgaccgca cgcgtggagc
     4141 agatcgtaga acggcgcgag cggcagttta tactgcgcaa tcacaccggc gagcttgccg
     4201 aacaggagcg gatgcaccgg cgccgcgcgc cgctgcgcta tcgcgtcgag gccggcgcgg
     4261 aagtcggcga ggcgggcatg ccggtcggcc gggctccagt cgccttcatc ggcgatgtcg
     4321 gcggcggtgc gaacaacgtg atagataatc tcgataggcg cgcgcagcgt cttcggcagc
     4381 aacaccctcg cgaccggaaa attttcgtat tgatcgacgt ccatccagcg agcctcgaaa
     4441 ccattctgta atgcggccgc caacacgcgc cgcaggacca cgtagtttaa gggttccgcc
     4501 aaaccgctgc agcgtgtaat cattgccgca tacggcacca atgccgcgcc ggacgccggc
     4561 ccgcggcgcg gcgccggccc aacaccttgg cggggcccga aagcaaagcc ggcctgatag
     4621 cgttagaatg cgcgtttggt tttgctgtac cggctcatgg ccggggctca agacgtcaac
     4681 taaatagatg atcgcggcga taaagtcaga attaccggct tcttcgaaac ccgcgtcagt
     4741 cggagttcgc caatttatgc gagtcatcct tgctcaaccc cgcggctttt gcgcgggtgt
     4801 tgtacgcgcc attgagatcg tcgatcgcgc gctacagcaa cacggggcgc ccgtgtatgt
     4861 tcgccacgag attgttcaca atcgccatgt ggtggacaat ctgcgtcaga aaggcgcgcg
     4921 ctttgtcgag gaactcgacg aggtaccgca cggcgccgtg gcgattttca gcgcccacgg
     4981 tgtcgcgcag acagtcgagc gcgacgcggc acagcggggc ctcgacgtgc ttgacgcgac
     5041 ctgcccgctc gtgaccaaag tccacgtgca agggcgccaa tatgtggccg ccggccgtat
     5101 gctgattctg atcggccacg ccggccatcc ggaagtggaa ggcacgatcg gccagattcc
     5161 gggcaaggtg cggctggtgc aaagcgaggc cgaagtcgcg catctggacc tgccgcacga
     5221 tacgccgctc gcgtacgtca cacagaccac cctgtcggtc gacgacacac gtggcattat
     5281 cgatgccttg ctgcgccgct tcaccgacat cgtcggtccc gacacgcgcg acatctgcta
     5341 tgccacgcaa aaccgccagg cggcggtgcg cgaactgagc aaagaggtcg acgtgctgtt
     5401 ggtggtgggc gcaaccaata gttcgaactc gaaccggctg cgcgagatcg gcagcgaaac
     5461 cggcgtggcg agctatctcg tcgccgacgg ttcggaagtg aagccggagt ggtttgcgaa
     5521 tgtgcagacc gtcggcatca cggccggtgc ttcggcaccg gaagaaatgg tgaagaacgt
     5581 gattgatgcg ctgcgcgcat tggggcccgt cgatgtcacg acgatggcgg gccgtgaaga
     5641 gaaagtcgaa ttcaagttgc cgtcgaaact gatgcaacca ctcgctgcac gcgaagttta
     5701 aggaggacgt cttgtctatt ccgctgctac agaaagtccg ggtcggcgca tacatcatgc
     5761 gtcagcatct ctccggtaac aaacgctacc cgctcgcgtt gatgttggag ccgctgttcc
     5821 gctgcaatct tgcctgcaat ggctgcggca agatcgatta cccggatccc atcctgaatc
     5881 agcgtctgtc gctcgaggaa tgccttggcg cagtcgacga atgcggcgca ccggtcgttt
     5941 ccattgccgg cggcgagccg ctgctgcaca aggaaatgcc gcagatcgtg aagggcatca
     6001 tcgcgcgcaa gaagttcgtg tatctgtgca cgaatgcgtt gctgatggaa aagaaaatgg
     6061 acgactacga gccgaatccg tatttcgtct ggtcggttca cctggacggc gacgagcagg
     6121 cgcacgatca ctccgtgtcg caggaaggcg tgtacgtcaa ggcagtcgcc gcgatcaagg
     6181 aagcgaagcg ccgcggtttc cgcgtgaaca tcaactgcac gctattcaac gacgccgtgc
     6241 cggaacgcgt cgctgcgttc ttcgacacac tgggcccaat gggcgtggac ggcatcacgg
     6301 tctcgccggg ttatgcctat gaacgcgcgc cggatcagca gcacttcctg aaccgcgaca
     6361 agaccaagca cctgttccgc gagatcttca agcgcggcaa caacggcaag aactggtcgt
     6421 tcagccagtc gggcatgttc ctcgacttcc tggccggcaa tcagacgtac gagtgcacgc
     6481 cttggggcaa cccggcgcgt accgtgttcg gctggcagaa gccgtgctac ctggtcggcg
     6541 aaggctacgt gaagaccttc aaggaattga tggaaaccac ggagtgggaa aagtacggta
     6601 cgggcaacta tgaaaagtgc gcggactgca tggtgcactg cggtttcgaa gcaacggccg
     6661 tgatggatac ggttgcgcat ccgctgaagg cgttgaaggt tagcctgcgt ggtccgaaga
     6721 cttccggcgc cttcgtcaag gatattgctt tggataagca gcgtccggcc gagtacgtgt
     6781 tctcgcggca cgtcgagatc aagctcgaag agattgggcg tacgggcaag ggcaaaaagg
     6841 tgcagacggc agcttccgct tccgcacact aagcttgctt aggatggact aagcggttcc
     6901 atccgcccaa tgacggacag gttttgcaaa aaaagcgcgg tagcttgatt gccaccgcgc
     6961 tttttctttg tgcgtgaatc ttttgcgtga cgtcagccgg cgctgcgcgc gttatgcgca
     7021 gtcaggaact tgatcagtcc atcaacgccg cccttggaaa gctgttcggc gaactgcgtc
     7081 tgataaacct gaatcagcca ggcgcccatc atgttgatgt cgtaaatctt ccaaccattg
     7141 gcgcccttgg tcagccggta accaatggca tcgtcgccgc cattgctgat cacgtgcgac
     7201 tgcacgacca gatcattcgc gccggcaggc acggtcaccg gtaagaactt gaatgtcaca
     7261 tcctgatcgc gcaactgcga cagcgatgcc gcgtacgtgc gcaccagcag caacgtaaac
     7321 tgttcgacca gttgcttctg ctgctcgggc gttgccgtgc tccatgcctt ccctaccgcg
     7381 atccgcgtgg tccgctgaaa atcggtagcc ggcacaaagc gcgtctgaac cagctcggtg
     7441 atcttcgcca tatcgccgcc acgcgcctgc ggatcagcct tcatcgcggc aaccgtgcct
     7501 tcgacggcac tcttcaccag cgcatcaggc gcgctttgcg cataggccgc ggtggacacc
     7561 acggccgcag ccagaaaagc agacagataa cgtttcatac gcttgccaaa gtcccaagaa
     7621 gtaagcgccg gcgggctcgc gagccagcgg cgaaagctga atagaccaag tataccgata
     7681 atgcgttccc gccccatggc cgccgaccgg gagccgtcct tgctaccgaa atggagccga
     7741 accgctccgg ctctgtatac ttgtgc
//
LOCUS       DATASH010000724         3921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3284188,
            whole genome shotgun sequence.
ACCESSION   DATASH010000724 DATASH010000000
VERSION     DATASH010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3284188"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..508
                     /locus_tag="VHV99_19125"
     CDS             182..508
                     /locus_tag="VHV99_19125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEX3637559.1"
                     /translation="MMKGQLAGLMKQAQQMQENMKKMQEELAQIEVEGQSGAGLVKVT
                     MTCKNDVRRVSIDPSLLADDKDMLEDLVAAAFNDAVRKAEATTQQKMSGMTAGMPMPP
                     GFKLPF"
     gene            553..1149
                     /gene="recR"
                     /locus_tag="VHV99_19130"
     CDS             553..1149
                     /gene="recR"
                     /locus_tag="VHV99_19130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEX3637560.1"
                     /translation="MKQPSALSALVEALRALPGVGPKSAQRMAYHLMQHDRPGAEKLG
                     RSLMFATEHLQHCEKCNTFTEAVICEVCSDSERDPELLCVVETPADQIMLEQTLTYRG
                     LYFVLMGRLSPLDGIGPKEIHFDRLIQRATDGLVKEIVLATNFTNEGEATAHYLAQTL
                     KSKGLKVTRLARGVPVGGELEYVDAGTIARAMLDRRSV"
     gene            1180..1404
                     /locus_tag="VHV99_19135"
     CDS             1180..1404
                     /locus_tag="VHV99_19135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HEX3637561.1"
                     /translation="MTEAEALALATHRHYKGGLYRVIGVARHSETEEPMIVYEHLWPH
                     DHGLWVRPAELFNGTLADGSPRFKALDIPL"
     gene            1485..2690
                     /locus_tag="VHV99_19140"
     CDS             1485..2690
                     /locus_tag="VHV99_19140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008349341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX3637562.1"
                     /translation="MSASTGPLAGVKVLELGTLIAGPFAARFMGEFGADVIKIEDPNG
                     GDPLRKWRKLYPEVGGTSLWWAVQARNKKSVTVNLKSPDGKEIVRRLAKEADIVVENF
                     RPGLLEKLGLGYEVLSAENPGLVMVRLSGYGQTGPYRDRPGFGAIAESMGGLRHITGY
                     PDLPPPRIGISIGDSIAALHGVIGALMALHHRQANGGKGQVVDVALYEAVFNMMESVV
                     PEYGVYGMIRERTGASLPGIVPSNTYPCRDGSIVIGGNSDPIFKRLMLAIGREDLGND
                     PTLASNDGRVPRTREIDDAIRVWLCTRSIDEALAVLNAADVPAGRIYSVADMFTDPQY
                     IARQMIQHFPWQDGREIALPNVTPKLSETPGETRWLGPQLGEHTDDVLQSLGYGPEEI
                     ARLRADKAI"
     gene            complement(2959..>3921)
                     /locus_tag="VHV99_19145"
     CDS             complement(2959..>3921)
                     /locus_tag="VHV99_19145"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HEX3637563.1"
                     /translation="RCNQYGPPESLVLETLPDLQPGPGQVVIDVRAASVNFPDVLVIQ
                     NKYQMKPDLPFTPGSEFAGVVRAVGPDVKRLKAGMRVAAYTAQGAFAEQALASEAACM
                     PLPDGIDFASAAAITLAYGTSHHAVVDRGALKAGETMLVLGAAGGVGLAAVEIGKALG
                     ARVIAAASSPEKLALCVEHGADAIIDYTSENLRERIKALTDGNGPDVIYDPVGGEYAE
                     PAFRSIGWRGRYLVVGFANGEIPKLPLNLALLKGASIVGVFWGQHIQREPDLASAAMQ
                     QIFGWIKDGRLRPHVTKRYALEQTAAALTDMASRRVSGKIVIEP"
BASE COUNT          780 a         1209 c         1224 g          708 t
ORIGIN      
        1 atcgcttatc cgcgaattcg gtgcggccat cgtttcgggc tcggtgaagc cgcttacgcc
       61 aatttcacgt gacacggggg ctggcgctcg ctgaaaaacc tgcgctcgcc cgctagcttg
      121 acgcttggca accggccagc cggtgccgca cttacatcca tacgattaaa ggagcacatc
      181 catgatgaaa ggccaactcg cagggctgat gaagcaagcc cagcagatgc aggaaaacat
      241 gaagaagatg caggaggagc tcgcgcagat cgaggtcgag ggccagtcgg gcgccggtct
      301 cgtgaaggtg acgatgacct gcaagaacga cgtgcgccgc gtgtcgatcg acccgagcct
      361 gctcgccgac gacaaggaca tgctggaaga cctcgtcgcc gccgcgttca acgacgccgt
      421 gcgtaaagcc gaagccacca cgcagcaaaa gatgagcggc atgacggccg gcatgccgat
      481 gccgccgggt ttcaagttgc cgttctaaat cgttctagac acgacaaccc tcagagcata
      541 cggccttagc gcatgaaaca accttccgcc ttgtcggcgc tcgtcgaagc gctgcgcgcg
      601 ttgccgggcg tgggtcccaa gtcggcgcag cgcatggcct atcacctgat gcagcacgac
      661 cggccgggcg ctgaaaaact cggccgctcg ctgatgttcg cgaccgagca tctgcagcac
      721 tgcgagaagt gcaacacgtt caccgaagcg gtgatctgcg aagtctgcag cgactccgaa
      781 cgcgatcccg aactgctctg tgtggtcgaa acgcccgccg accagatcat gctcgaacag
      841 acgctgacat accgcggact gtatttcgtg ctgatgggac ggctcagtcc gcttgatggt
      901 atcgggccga aagagatcca tttcgatcgc ctgatccagc gcgccacaga cggcctcgtg
      961 aaggaaatcg tgctcgccac gaactttacg aacgagggcg aggcgaccgc gcattacctc
     1021 gcacagacgc tcaagtcgaa gggcctgaag gtgacgcgcc tcgcgcgcgg cgtgccggta
     1081 ggcggcgaac tcgaatacgt cgacgcgggc acgatcgcgc gtgcaatgct cgacaggcgc
     1141 tcagtctaga cagcagttac ctcgcaacaa ggcaacaata tgaccgaagc agaagcattg
     1201 gcgctcgcca cgcaccggca ttacaagggc ggtttatatc gcgtgatcgg cgtggcgcgg
     1261 cattcggaga cggaagagcc gatgatcgtc tatgagcatc tctggccaca cgatcatggc
     1321 ttgtgggtac gtcctgcgga gctgttcaac ggcacgctcg ccgacggttc gccgcggttc
     1381 aaggcgctcg acattccact gtaaaggctg tgtggcgctt caagccatca cgctatttca
     1441 agccgctgtg aaaccgcttt gaaaacgaca acgaaggaga caccatgagc gcttctacgg
     1501 gaccactcgc cggcgtcaag gtgctcgaac tcggtacgct gatcgccggg ccgttcgccg
     1561 cgcgcttcat gggcgagttc ggcgccgacg tgatcaagat cgaagatccg aacggcggcg
     1621 atccgctgcg caaatggcgc aagctgtatc cggaagtagg cgggacatcg ctatggtggg
     1681 ccgtccaggc acgcaataaa aagtcggtga cggtcaatct caagtctccc gacggcaagg
     1741 aaatcgtccg gcggctggcg aaggaagccg atatcgtcgt cgagaatttc cggccgggct
     1801 tgctcgaaaa actcgggctg ggctatgagg tgttatcggc cgaaaacccc ggcctcgtga
     1861 tggtgcggct gtctggatac ggccagaccg ggccgtatcg cgaccggccg ggctttggcg
     1921 ccatcgctga atcgatgggc ggattgcgtc acatcacggg ttacccggac ttgccgccgc
     1981 cgcgcatcgg catttccatt ggtgactcca ttgcagcgct acatggcgtg attggcgcgt
     2041 tgatggcgct gcatcatcgg caagcgaatg gcggcaaagg acaagtagtc gatgtcgcgc
     2101 tgtacgaagc tgtcttcaat atgatggaaa gcgtcgtacc cgaatacggt gtgtacggca
     2161 tgatccgcga gcgcaccggc gcgtcgttgc cgggcatcgt gccgtcgaac acgtacccgt
     2221 gccgcgacgg cagtattgtg attgggggca acagcgatcc aatcttcaaa cggctgatgc
     2281 tcgcgatcgg ccgcgaggac ctgggcaacg atcccacgct ggcatcgaat gacgggcggg
     2341 tgccgcgcac acgggaaatt gacgacgcta tccgcgtatg gctttgcacc cgttccatcg
     2401 acgaggctct cgctgtactg aatgccgcgg acgtgcccgc cggccgaatc tacagcgtgg
     2461 ccgatatgtt caccgatccg caatacatcg cacgccagat gatccagcat tttccttggc
     2521 aggacggtcg ggagatcgca ctgccgaacg tcacgccgaa attgtcggag acgccgggcg
     2581 agacgcgctg gctaggcccg caactcggtg aacacaccga tgacgtcctg caatcgctcg
     2641 ggtatgggcc tgaagagatt gcgcgactgc gggcggacaa ggcaatttaa gcttttccac
     2701 gtagcgatcc gcccaaaata aagactctcg gcacgcgggg ttggctacct gatcctgcaa
     2761 gccgaacgta ccttgccatc aacacagtgc tcggcggcat tgacggcggg cggggctcga
     2821 ctggatcgcg tgacgctggc cgagcgcctg atgaattggc agcggagcac cgtcgacgcc
     2881 gaaagcagtt gaggcgcggc aggtagcttg ttaagtgttg caggcaagcg cagccatgat
     2941 tcgccgcttc acgattcatc aaggttcgat cacgatcttc cccgacacgc gtcggctggc
     3001 catgtcagtc aatgctgcgg cagtttgttc aagggcgtag cgcttcgtga cgtgcggccg
     3061 tagccggccg tccttgatcc agccgaaaat ctgctgcatc gcggcactcg ccaggtctgg
     3121 ctcgcgctgg atatgctggc cccagaacac gcccacaata ctcgcgccct tgagcagcgc
     3181 gaggttgagc ggcagcttcg gaatttcgcc attcgcgaac ccgaccacaa gataccggcc
     3241 gcgccacccg atactgcgaa aagccggctc cgcgtactcg ccgccgaccg ggtcgtagat
     3301 gacgtcgggc ccgttcccgt ctgtcagcgc cttgatccgt tcgcgcaggt tttcactcgt
     3361 gtagtcgatg atggcgtccg caccatgttc cacgcacaac gcgagctttt ccggacttga
     3421 cgcggccgca atcacgcgcg caccgagcgc cttgccgatt tccaccgcgg ccagccccac
     3481 gccgcccgcc gcccccagca cgagcatggt ttcgccggcc ttcagtgcgc ctcggtcgac
     3541 cacggcgtgg tgcgatgtgc cgtacgcgag cgtgatggcc gcagcgctgg cgaagtcgat
     3601 gccgtcgggc aatggcatac atgccgcttc gcttgccagc gcctgctcgg cgaacgcgcc
     3661 ttgcgccgtg tacgcggcga ctcgcatgcc ggccttcaat cgcttgacgt ccgggcctac
     3721 ggctcgcacc acacccgcga actcggaacc cggtgtgaat ggcaggtcgg gcttcatctg
     3781 atacttgttc tggatcacga ggacgtcagg gaagttcacg ctggcggcgc gtacgtcgat
     3841 tactacctgg cccggcccgg gctggagatc gggcagtgtt tccaggacga gggactccgg
     3901 tggtccgtac tggttgcatc g
//
LOCUS       DATASH010000725         3469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6842096,
            whole genome shotgun sequence.
ACCESSION   DATASH010000725 DATASH010000000
VERSION     DATASH010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6842096"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VHV99_19150"
     CDS             complement(<1..193)
                     /locus_tag="VHV99_19150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013698706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX3637564.1"
                     /translation="MPAQEKSPTPPTAASAWIEQWSHLVEQGGAVLDVASGNGRHARY
                     FALRGHPVLALDRDETALAN"
     gene            complement(202..1404)
                     /locus_tag="VHV99_19155"
     CDS             complement(202..1404)
                     /locus_tag="VHV99_19155"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX3637565.1"
                     /translation="MFPDRIFSGMRPTGSLHLGHYHGVLKNWVRLQSEYPCFFAVVDW
                     HALTTHYETPEVIEKNVWEVLIDWLASGIDPAQATLFIQSRVPEHAELALLLGMSTPL
                     GWLERVPTYKEQIEKLKEKDLTTYGFLGYPVLMAADILLYRASLVPVGEDQVPHVEMT
                     REIARRFNYLYGREADFEEKALDAAKKLGGKRAKLYHELRVAYQQEGDDEALEKARAM
                     LQESQSLSMNDRERLFGYLEGARKIILVEPQALLTEASRMPGLDGAKMSKSYGNTIGL
                     REDAETITKKVRTMPTDPARVRRTDPGDPDKCPVWQLHQVYTDETTHKWVQTNCRSAG
                     IGCLECKQPVIEGILREQQPMLERAQKYMDDPSLLRAIVADGCDKARKFAVETMRDVR
                     DAMGLSYN"
     gene            complement(1409..2074)
                     /locus_tag="VHV99_19160"
     CDS             complement(1409..2074)
                     /locus_tag="VHV99_19160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007580401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX3637566.1"
                     /translation="MDSSLIQTIAVYALPVIFAITLHEAAHGYAARLLGDNTAYVLGR
                     VSLNPMRHIDPVGTLAIPLILYFVTSGAFMFGYAKPVPVAFGNLRNPRWGSLWVAAAG
                     PASNFAQALIWGVIAVLLASFNVSEPFFTRMAAAGVGVNLVLGVLNLFPLPPLDGGRV
                     LMALLPVRASIMLSRLEPYGFFIVMALVMTGTLTRFWLGPLVSAGYVAVSAVLNPLAS
                     LLQ"
     gene            complement(2248..2886)
                     /locus_tag="VHV99_19165"
     CDS             complement(2248..2886)
                     /locus_tag="VHV99_19165"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX3637567.1"
                     /translation="MSQFFRIHPDNPQPRLINQAVQIINDGGIVALPTDSSYALACHL
                     DDKNAVERIRRIRGLDEKQLLSLLVRDLSELSNFAIVDNRNYRLIKSVTPGPYVFVLQ
                     ATKEVPRRLSHPSRKTIGLRVPNHAITLALLEALGQPLLGSTLILPPDTHPLNDAEEI
                     RQRLEKQIDLVIDAGACPMEPSTVIDLTGEEPVLVRPGLGSLEPFGITAENA"
     gene            complement(3257..>3469)
                     /locus_tag="VHV99_19170"
     CDS             complement(3257..>3469)
                     /locus_tag="VHV99_19170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014723269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HEX3637568.1"
                     /translation="EFIQLGGRAIEVVTGSHTPDQYREYAEVARRYGFLASRGSDFHA
                     AGEGRIDLGQLPQLPADLKPVWECLV"
BASE COUNT          709 a         1048 c         1050 g          662 t
ORIGIN      
        1 ggttcgccaa ggcggtttca tcgcgatcca gcgccagcac cggatgcccg cgcaacgcga
       61 aataacgcgc atggcggcca ttgcccgacg caacgtcgag taccgcgccg ccctgctcca
      121 ccagatgcga ccattgctca atccatgcac tggcggcggt aggaggcgtg ggcgactttt
      181 cttgcgctgg cattgcggtc atcagttata cgagaggccc atcgcgtcgc ggacgtcacg
      241 catggtctct accgcgaact tgcgcgcctt gtcgcaaccg tccgccacga tcgcgcgcaa
      301 cagcgacgga tcgtccatgt atttctgcgc gcgctccagc atgggttgct gctcgcgcaa
      361 gatgccttcg atcaccggct gcttgcattc gaggcaacca attccggcgc tgcggcagtt
      421 cgtctgcacc cacttgtgcg tggtttcatc ggtatagacc tgatgcagtt gccacaccgg
      481 acacttgtcc gggtcgcctg gatcagtgcg gcgaacccgc gccggatcgg tcggcatggt
      541 gcgtactttc ttcgtgatgg tctcggcgtc ttcacgcaag ccgatggtat tgccatacga
      601 cttcgacatc ttcgcgccat cgagtcccgg catgcgcgac gcttcggtca gcaacgcctg
      661 cggctcgacc agaatgatct tgcgcgcgcc ttccaggtat ccgaacagcc gttcgcgatc
      721 attcatggag aggctttgcg actcctgcag catcgcacgc gccttttcga gcgcttcgtc
      781 gtcgccttcc tgctgatacg cgactcgcaa ctcgtgataa agcttcgcgc gtttgccacc
      841 gagtttcttc gcagcatcga gcgccttctc ttcgaaatcc gcctcgcgcc cgtacagata
      901 attaaagcgc cgcgcgatct cgcgcgtcat ttccacgtgc ggcacctggt cctcgccgac
      961 cggcacgagc gacgcgcggt acagcaggat gtcggcggcc atcagcaccg gataaccgag
     1021 aaaaccgtac gtggtcaggt ccttttcctt cagcttctcg atctgctctt tgtaagtcgg
     1081 cacgcgttcg agccagccga gcggcgtact catgccgagc agcagcgcaa gctcggcgtg
     1141 ttccggcacg cggctctgga taaagagcgt ggcctgcgcc ggatcgatgc ccgacgcgag
     1201 ccagtcgatc agcacttccc agacgttttt ctcgataact tcaggcgttt cgtagtgggt
     1261 cgtcagcgca tgccagtcga cgaccgcgaa aaagcatggg tactcggact gcagccgcac
     1321 ccagttcttg agcacgccgt gatagtggcc tagatgcagc gagccggtcg gccgcatgcc
     1381 agagaagata cggtcaggga acatggattt attgtaagag cgaggcaagg ggattcagga
     1441 cggccgacac cgcgacatac ccggcgctca ccagcggccc gagccagaaa cgcgtcaaag
     1501 tgccggtcat gacaagtgcc atcacgatga aaaaaccgta aggttcaagc cgcgacagca
     1561 tgatcgacgc gcgcacgggc agcagcgcca tcagtacacg tccaccatca aggggcggca
     1621 ggggaaacag attgagcacg ccgagcacga gattcacgcc aacaccggcc gcagccatgc
     1681 gcgtaaaaaa tggttcgctg acgttaaacg acgccagcag caccgcgatc acgccccaga
     1741 tcagcgcctg ggcgaagttg ctcgcggggc cggccgccgc gacccacaag ctgccccagc
     1801 gcggattgcg caggttcccg aatgccaccg gcaccggctt cgcgtaaccg aacatgaacg
     1861 cgccactcgt gacgaaataa aggatcagcg gaatagcaag cgtaccgacg ggatcgatgt
     1921 ggcgcattgg gttgagcgac acgcgaccga gcacgtaagc cgtgttgtca cccagcagac
     1981 gggccgcata gccgtgagcg gcctcatgca gcgtgatcgc gaagatcacg gggagcgcgt
     2041 agaccgcgat ggtttgtatc agggaggaat ccatagctcg ctattgtatc aagcagttgt
     2101 ggcgccactc gttggcggac ggtttggcgt tcgcccaggg cgatcgggcg cgagcgtcga
     2161 ggcagtgagg ctgcggcagt gagtctgcgc caggggcaat tcgcccgaac gcagcgtcac
     2221 tgcgtattgt gtcatcgtgc ggggttttca ggcgttttca gcggtaatac cgaatggttc
     2281 cagactgccc aggccgggcc gcaccagcac gggttcctca ccggtcaggt caatcaccgt
     2341 ggacggttcc atcgggcatg cgccggcgtc gatgaccagg tcgatctgtt tttccagccg
     2401 ctggcgaatc tcttcggcgt cgttgagggg gtgggtgtcg ggcggcagga tcagcgtcga
     2461 gccgagcagc ggctgaccca gcgcttcgag caacgcgagc gtgatcgcat gattcggcac
     2521 acgcaggccg atcgtctttc gcgacgggtg cgacaggcgc cgcggcactt ccttcgtcgc
     2581 ctgaagcaca aacacgtaag gaccgggcgt aaccgatttg atcaggcgat agttacggtt
     2641 atcgacgatc gcgaaattcg acaattccga taaatcgcgc actagcagcg agagcagttg
     2701 tttctcgtcg agcccgcgaa tccggcggat ccgttcaacc gcatttttat cgtcgagatg
     2761 gcacgcgagc gcgtagcttg aatcggtcgg cagcgcgaca atgccaccat cattgatgat
     2821 ctgcaccgct tggttgatca gtcgcggctg cggattgtcc ggatgaattc gaaagaattg
     2881 agacatgacc agatagacgt aagtgggttg tccggaagct tgctgccaac cgtcgccgat
     2941 acgctgggcg tacgcgttgc gttttatgtt tttcgcgccg ggtatgacgt ccgcggcttc
     3001 gaactgattt caaactgcat tcgaaccgaa tatcagatta aagcttcgaa gcagctccca
     3061 tgcgaacacg cgagaatacc cgcaaatgca tgcagttgcg cgacaaacag aagtgagtga
     3121 atgcaaggat cgtgcgcagc gtttacctgg ttcaaacgag cgggttcaaa cgagcgagtt
     3181 taaacaaggg ggttcaaacg agcttcaata aagcgatcca acgcgcagcc cgactatgaa
     3241 ccggcgcggt gcggctttaa accaggcact cccacacggg cttgagatcg gcgggcagtt
     3301 gcggcaactg gcctaggtcg atacgcccct cgcctgccgc atggaaatcc gagccgcgcg
     3361 atgcgagaaa gccgtaccgc cgggcgactt ccgcatattc gcgatactgg tccggcgtgt
     3421 gactgccggt cacgacttca attgcacggc cgccgagctg gatgaattc
//
LOCUS       DATASH010000726         2220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3875351,
            whole genome shotgun sequence.
ACCESSION   DATASH010000726 DATASH010000000
VERSION     DATASH010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3875351"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..739
                     /locus_tag="VHV99_19175"
     CDS             143..739
                     /locus_tag="VHV99_19175"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HEX3637569.1"
                     /translation="MNESVAKTDPSRSSHYVSDALYRALIENFSNWQDDARAVTDVAA
                     RDEFRRLIEREARLLDQLRYDDWLAMYAAECIYWVPSTPGAGDPRREISVMFDDRRRL
                     EDRIYRMRTGFAWSQAPASRTVRLVSNVEVFATAREDTRMVRSNFLISEFWGDETRVL
                     TGWAGHRVMRDCAGWKIQAKQVNLIDCDQSIRNPSIIL"
     gene            765..1730
                     /locus_tag="VHV99_19180"
     CDS             765..1730
                     /locus_tag="VHV99_19180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3637570.1"
                     /translation="MACGRYDRTQPLIDGRVEPEGVDLTFIPLRPGETFWRMLNHEEF
                     DASEMSLSSYTILRSEGDTRFIAIPVFPSRVFRHSAVYLRADSKIEKPQELKGKRIGV
                     GDYQMTAAVWVRGFLKADYGVNAEDVTWVIGRPVRTIKPPDGIRVEFMKPETTLEDML
                     ERGEIDALASVMIPKTLGTTVRRLFSDFRNVERDYYQRTKIFPIMHTFVLKAKLYEDN
                     PWLAISFYRAFCRARDIAYHWMYDTDALTVSLPWVIDHLEATRKIFGPQVWDYSIEGS
                     RPTLDALMTHLDEQKLTRRRMKVEELFVPNISPFLNEYLRATAEE"
     gene            1790..>2220
                     /locus_tag="VHV99_19185"
     CDS             1790..>2220
                     /locus_tag="VHV99_19185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637571.1"
                     /translation="MTNDARVSRRGALKAGVALAAGAAAGSVLSETAVADDVRRFDHQ
                     QSLDPKSRTLLKGGTIVSMDAKVGDLVKGDLLIEGTKISAIAPEINAADAQVVDAADT
                     IIVPGLVDCHRHSWEGQLRRINPNSPTLADYMNATHLSFAK"
BASE COUNT          460 a          717 c          649 g          394 t
ORIGIN      
        1 atccccgatc gcgtcaaaac ccacgacgat ggctcgatca ccgcgccggc gaccgacgag
       61 gccttcatgc gcgagcactt caaggagtgg aagcggctga tgaaggcgcg cccaaacctc
      121 gccatccagc gcgagccgtg acatgaacga aagcgtcgca aaaaccgacc cgtcgcgcag
      181 ctcgcactac gtcagcgacg cgctctatcg cgcgctcatc gaaaattttt ccaactggca
      241 ggacgatgcg cgcgccgtca ccgacgtcgc cgcgcgcgac gaatttcgcc gcctcatcga
      301 acgcgaggcg cgcctgctcg accagctgcg ctatgacgac tggctcgcca tgtacgccgc
      361 cgagtgcatc tactgggtgc cgtcgacgcc gggcgccggc gatccgcggc gcgagatttc
      421 ggtgatgttc gacgatcgtc gccggctcga ggatcggatc taccggatgc gcactgggtt
      481 cgcctggtcg caggcaccgg cgtcgcgcac ggtgcgtctc gtcagcaatg tcgaagtttt
      541 tgcgaccgcc cgcgaggata cacgcatggt gcgctcgaac ttcttgatca gcgaattctg
      601 gggtgacgaa accagagtac tcaccggctg ggccgggcat agagtcatgc gcgactgcgc
      661 gggctggaag atccaagcca agcaagtcaa cctgatcgat tgcgaccaaa gcattcgcaa
      721 tccaagcatc atcctgtgaa gccatgaaaa gattgaagtt gacgatggcc tgcggccgtt
      781 acgaccgcac gcaaccgctg atcgacggcc gggtcgagcc cgaaggcgtc gacctgacat
      841 tcattccgct gcggcctggc gagacgttct ggcgcatgct caatcacgaa gagttcgacg
      901 cctccgaaat gtcgctgtcg tcctacacca tcctgcgctc ggagggcgac acgcggttca
      961 tcgccattcc ggtgtttccg tcgcgagttt ttcgccattc cgcggtttat ctgcgcgccg
     1021 attccaagat cgagaagcca caggagctga aaggcaagcg catcggcgtc ggcgattatc
     1081 agatgacggc tgcggtgtgg gtgcgcggct ttctcaaagc tgactatgga gtcaacgccg
     1141 aggacgtcac ttgggtgatc ggccgcccgg tgcgcaccat caagccgccg gatggcatcc
     1201 gcgtcgaatt catgaagccg gaaacgacgc tggaagacat gctggaacgc ggcgagatcg
     1261 acgcgcttgc ctcggtcatg atcccgaaga cgctcggcac cacggtacgg cggctgtttt
     1321 ccgattttcg caacgtcgag cgcgattact accagcgcac caaaatcttt ccgatcatgc
     1381 acaccttcgt gctcaaggcc aagctttacg aggacaaccc ctggctcgcc atcagcttct
     1441 atcgcgcctt ctgccgcgcc cgcgacatcg cttatcactg gatgtacgac accgacgcac
     1501 tgaccgtgag cttgccctgg gtgatcgacc acctggaagc gacgcgcaaa atcttcggcc
     1561 cacaagtctg ggattattcg atcgagggca gccgcccaac gctcgatgcg ctcatgaccc
     1621 acctcgacga gcagaagctg acgcgccggc gcatgaaggt cgaggaattg ttcgttccga
     1681 atatcagccc cttcctcaac gaatatctgc gcgcgaccgc cgaagaataa ccgcgcaact
     1741 tgcgctaagc ttacgtctcg tgaacgacaa aaccacggga ggaccgatca tgaccaacga
     1801 cgcacgcgtg tcgcgccgcg gcgcgctcaa ggccggcgtc gcgcttgcag caggcgccgc
     1861 agccggcagc gtcctatccg aaaccgcagt ggcggacgac gtgcgccgct ttgatcatca
     1921 gcaaagcctc gatccgaaga gccgcactct gctcaagggc ggcaccatcg tcagcatgga
     1981 cgccaaagtc ggtgacctgg tgaaaggcga tctgttgatc gaaggcacga aaatctccgc
     2041 catcgcgccc gagatcaatg ccgccgatgc gcaggtggtc gatgcggccg acaccatcat
     2101 cgtgccgggt ctggtcgact gccatcggca ttcctgggaa ggtcagctgc gccgcatcaa
     2161 tccgaattcg ccgacgctcg cggactatat gaacgccacg cacctctcgt tcgctaaatt
//
LOCUS       DATASH010000727         3045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2491761,
            whole genome shotgun sequence.
ACCESSION   DATASH010000727 DATASH010000000
VERSION     DATASH010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2491761"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..1180
                     /locus_tag="VHV99_19190"
     CDS             203..1180
                     /locus_tag="VHV99_19190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3637572.1"
                     /translation="MTTAMVKQEIAVASFSRVYDLDQVETALNDLGEGANEALRSTYE
                     KMLKTGNLRFCVKPNRMPSIDDLIDALPNFSAPLDDIRRQVALCLETEDRLELMPILL
                     LGDPGIGKTHFAKQLARLLGTAYQYVAMSSLTAGWILSGASSQWKNAKPGKVFDALVN
                     GSYANPVITVDEIDKASGDSQYDPLGALYALLEHDTAQSFTDEFAEIPINAGHVIWIA
                     TANDERSIPEPILNRMNVYEIPPPDHDGARRIAQSIYGEIRSAHNWGLRFPETLGDAA
                     LDALKRASPREMRRAILNGFGAARIAGRDQIDASDIRLDYGNRRKPIGF"
     gene            1612..2718
                     /locus_tag="VHV99_19195"
     CDS             1612..2718
                     /locus_tag="VHV99_19195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX3637573.1"
                     /translation="MDQIECVVIGAGVIGLAVARALAARGREVIVLEAAEAIGVGTSS
                     RNSEVIHAGIYYPRGSLKAALCVRGRELLYDYCVEHNVPHSRCGKLLVATSRNQIPQL
                     ESIMAKGRDNGVLDLMRITGDQAQALEPALECVEAVFSPQTGIVDSHQLMLALQGDAE
                     RDGAVCAFHAPVEAIEASNGRFIIKVGGSTPTTISAACVINSAGLQANALARKIRGLD
                     ARHVPPLYLARGNYFSISGRAPFSRLIYPMPNEAGLGVHLTIDLGGQARFGPDVEWVD
                     AVNYDVDPRRADSFYAAIRAYWPALPDDALQPAYAGIRPKLSGPGEPAADFVIQGPAA
                     HGVRGLVNLFGIESPGLTASLAIAQRVCELSGRA"
     gene            2957..>3045
                     /locus_tag="VHV99_19200"
     CDS             2957..>3045
                     /locus_tag="VHV99_19200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014895772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="High potential iron-sulfur protein"
                     /protein_id="HEX3637574.1"
                     /translation="MKSSRRTFLITSIGVASTFALSRQAFADAP"
BASE COUNT          580 a          928 c          943 g          594 t
ORIGIN      
        1 ttactcgggg cgtcctccaa gaggacttac tcgcggcact cccctcaagg gcacgtcctc
       61 cgggcatcac cgaaagcgag ctgttgttag cagcagagcc gtacgactgc tggcttgcac
      121 acgaatatgt cctcgataca ctggacttga aacggcgccc accaggcgcc gccgcagcaa
      181 gaccagccag acggggtgag cgatgacaac agcaatggtc aaacaggaaa tcgccgtggc
      241 gtctttcagc cgggtgtacg acctcgatca ggtcgagacc gcgctgaacg atctcggcga
      301 aggcgcgaac gaagcgctgc gcagcacata cgaaaagatg ctgaaaaccg gcaatctgcg
      361 cttctgcgtc aagccgaacc ggatgccgtc gatcgacgat ctgatcgacg cgctccccaa
      421 cttttccgcg ccgctcgacg acatccgcag gcaggtcgcc ttgtgcctcg agaccgaaga
      481 ccggctcgaa ctgatgccga tcctgctgct cggcgacccc ggcatcggca agacgcactt
      541 tgccaagcag ttggcgcgcc tgctcggcac cgcctatcag tacgtagcga tgagttcgct
      601 gaccgcaggg tggatccttt cgggcgcctc gtcgcaatgg aagaacgcga aaccgggcaa
      661 ggtgttcgat gcgctcgtga acggcagtta cgcgaacccc gtgatcacgg tcgacgaaat
      721 cgacaaggcc agcggcgatt cgcaatacga tccgctcggc gcgctttacg cgttgctcga
      781 gcacgacacg gcgcaaagct tcaccgacga attcgctgag attccgatca atgccggcca
      841 cgtaatctgg attgcgaccg cgaacgacga gcgctcgatt cccgagccga tcctgaaccg
      901 gatgaacgtc tacgagattc cgccgccgga tcatgacggc gcgcgccgta tcgctcaatc
      961 gatctatggc gagattcgtt cagcgcataa ctggggtctg cgctttcccg aaacgctagg
     1021 cgacgcggcg ctcgatgcgc tgaagcgggc atcgccgcgt gaaatgcggc gcgcgattct
     1081 gaatggattc ggcgcggcgc gcattgccgg ccgcgatcag attgacgcca gcgatattcg
     1141 gctggattac ggaaatcgtc gaaaaccaat cggtttttga ggtttttcgc ctgtttttgg
     1201 ctgaaaattg ccttctggac ggctttgggg cagcgccaaa tcgggtgtct ataccgaaaa
     1261 tcggatgaat gcttcgacaa ttgtttctaa tcgtgaagtg ggactgcgta ccgccgaatc
     1321 gcgcgatgcg ccgtatcgct cgcatgaaga atcgagcgcg gcgactttcg acggcgtaca
     1381 ctggctgtct cacgcccaga cgttcaagac ctgctcaaga cagaaatgcc cgcagttatc
     1441 cgactgcggg catttctgtc tgtgcagcgt cgtacaattt tgcaaagcgg ttccatcagg
     1501 gcagggtgcg ttcgacaacg agcggtgtgc ggttctcgat agccgcatcc tgtcttcatc
     1561 gatatcggct gaacgaggta gcgcgcggcg aggtcagccg cgccaaggga catggatcaa
     1621 atcgaatgtg tagtgatcgg cgcaggcgtg atcggtctcg ccgtggcacg cgcactggcg
     1681 gcgcgcgggc gcgaggtgat cgtgctggaa gcggccgagg cgatcggcgt gggcaccagc
     1741 tcgcgcaata gcgaagtgat acacgcgggg atctactatc cacgcggttc gctgaaagca
     1801 gcgctctgtg tgcgtggccg cgagctgctc tatgactatt gcgtcgagca caacgtgccg
     1861 cattcgcgtt gcggcaaact actggtggcc acttcgcgca atcagattcc tcagctcgaa
     1921 agcatcatgg cgaaggggcg cgacaacggc gtactcgatc tgatgcggat taccggcgac
     1981 caggcgcagg cgctcgagcc cgcgctcgaa tgcgtggagg cggtgttctc gccgcagacg
     2041 ggcatcgtcg atagtcatca gttgatgctg gcgcttcagg gtgacgccga gcgtgacgga
     2101 gcggtgtgtg cgttccatgc gccggtcgag gcaatcgagg cgagcaacgg ccgcttcatc
     2161 atcaaagtgg gcggcagcac gccaaccacg atcagcgccg cttgcgtgat caacagcgcc
     2221 ggtttgcaag ccaacgcgct ggcacgcaag attcgcggtc tggacgcgcg ccatgtgccg
     2281 ccgctttatc tcgcgcgcgg caactacttc agcatctcgg ggcgcgcccc gttcagccgg
     2341 ctgatctatc cgatgccgaa cgaagccggt ctcggcgtgc atctgacgat cgatctcggc
     2401 ggccaggcgc gtttcggtcc cgacgtcgaa tgggtcgatg cggtcaatta cgacgtcgat
     2461 ccgcggcgtg ccgattcctt ttacgcggcg attcgcgctt actggcccgc acttccggac
     2521 gatgcgctgc agcccgccta tgcggggatt cgtccgaagc tttccggccc gggcgagccc
     2581 gccgccgact tcgtgattca aggtccggcc gcgcatggcg tgcgcgggct cgtcaatctg
     2641 ttcggtatcg agtcgccggg attgacggcg tcgctcgcga tcgcgcagcg cgtctgcgaa
     2701 ctgagcgggc gcgcttaaca ggtgcgctta acgggcgtac ttgacgggcg cacttggccg
     2761 ccataccccc gagccctcat gtcgcgttgg cgtctcatgt gaacaacccg ccttccgcgc
     2821 ccgactttcg ggcgcgaggt cgtaccaatt cggttatctc tatcatttgg gccgtcacgc
     2881 gcgacggctt cattgctatc cttggggacc gctgtcaata gaacggcgat cagttcaata
     2941 taaatggagt gtccccatga agtcttcccg tcgtacgttt ttgatcacca gcattggtgt
     3001 ggcatccacc ttcgcgctgt cgcgccaggc tttcgccgac gcacc
//
LOCUS       DATASH010000728         4916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_117767,
            whole genome shotgun sequence.
ACCESSION   DATASH010000728 DATASH010000000
VERSION     DATASH010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4916
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117767"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..891
                     /locus_tag="VHV99_19205"
     CDS             43..891
                     /locus_tag="VHV99_19205"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HEX3637575.1"
                     /translation="MKLLRYGPKGQEKPGLLDAQGKIRDLSKVVPDIDGAALSDESLA
                     KLRALDPASLPVVEGNPRIGPCVGKIGKFICIGLNYADHAAESNLPVPTEPVIFNKWT
                     SAINGPNDDVEIPRGSKKTDWEVELGVVIGKPAKYIDEANALDYVAGYCVINDVSERE
                     WQIERGGTWDKGKGFDTFGPIGPWVVTRDEVADPQNLSLWLEVDGHRYQNGSTKTMVF
                     GVAKLVSYVSQCMSLQPGDVISTGTPPGVGMGVKPNPVYLKPGQTIRLGVEGLGEQTQ
                     KTYAAE"
     gene            complement(1124..1558)
                     /locus_tag="VHV99_19210"
     CDS             complement(1124..1558)
                     /locus_tag="VHV99_19210"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX3637576.1"
                     /translation="MTDLLDRARGALHAQPFSMLLGAELMHTGTDELTLCLPIRDELR
                     QQRGFVHGGVISYLADNALTFAGALSLGPKVVTSEYKINYLRPVVNGTLIARARVVYT
                     GRSQATCQCNVFVMDDGREKLVAVAQGTINRIGDMGEHEPAG"
     gene            1823..2060
                     /locus_tag="VHV99_19215"
                     /pseudo
     CDS             1823..2060
                     /locus_tag="VHV99_19215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013342113.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            complement(2173..2376)
                     /locus_tag="VHV99_19220"
     CDS             complement(2173..2376)
                     /locus_tag="VHV99_19220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX3637577.1"
                     /translation="MATGTVKWFNDAKGFGFITPDDGGEDLFAHFSEVQGSGFKSLQE
                     NQKVTFEVKQGPKGKQAANIQPA"
     gene            2606..2881
                     /locus_tag="VHV99_19225"
     CDS             2606..2881
                     /locus_tag="VHV99_19225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637578.1"
                     /translation="MTHYLQEELTSFLSRLTPYLAILESKSPQEGAPDPVGRITVDSD
                     HHQAFALHTTDKKPHGDTSETPTGQSATYTRFKTADADWGPWQREEY"
     gene            complement(2935..4044)
                     /locus_tag="VHV99_19230"
     CDS             complement(2935..4044)
                     /locus_tag="VHV99_19230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HEX3637579.1"
                     /translation="MQSMTPSTASTSTLATALRDHFTRVVLPLWRGPGFNPALKLPYE
                     AVSAEGSEPLPAQRYRAMACARQLFVFSQADDAAHAQVLFDSLMHTFQDPRHGGWFYS
                     VDAQGAPLDTTKDLYTHAFVVFACAEYGSRSGNRDALEVVHRTSALIQSQFAAEGDLF
                     NAALTADFAKVVGTPIQNPLMHLTEAWLAARAATHDNAFDAALRRLASAIARHFVHAP
                     SGCIAELPIGADDNRLEPGHQFEWFWLVKQAGSLFEASGLDESLTRAFSFAQQYGVDP
                     QTGGVCASLDETGRIKDATQRIWAQTEYLRALASHHDPAALAALPRQIELFRQRFLRP
                     QGWFECKTAAGGVARADMPSTTPYHLATAYEALPG"
     gene            4310..4423
                     /locus_tag="VHV99_19235"
                     /pseudo
     CDS             4310..4423
                     /locus_tag="VHV99_19235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            4557..>4916
                     /locus_tag="VHV99_19240"
     CDS             4557..>4916
                     /locus_tag="VHV99_19240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637580.1"
                     /translation="MADSVASTQSRPQRAASKKRRQATVSTETENKLARAARSAQRLA
                     QLSDASRDDSTLDLFPDDPTRATLEAMNIDVRQGTLHGFELPDVVLAAVGAIDADGAS
                     FEARPARRAPRAAKSDEP"
BASE COUNT         1009 a         1440 c         1535 g          932 t
ORIGIN      
        1 acacacgaac caaatatcac tgaacgtaag ggaagtgcaa cgatgaaact gcttcgttat
       61 gggccgaagg gccaggaaaa gccgggcctg cttgatgcgc agggcaagat tcgcgatctg
      121 tcgaaagtgg tgcccgatat cgacggcgcc gcgctgagcg acgaaagcct cgccaaactg
      181 cgcgcgctcg atccggcctc gctgccggtc gtggaaggca acccgcgcat cggcccgtgc
      241 gtgggcaaga tcggcaagtt catctgtatc ggcctgaact acgcggacca cgcggccgaa
      301 tcgaatctgc cggttcccac ggagccggtc attttcaaca agtggacgag cgcgatcaac
      361 ggcccgaacg acgacgtcga aatcccgcgc ggctcgaaga agaccgattg ggaagtcgaa
      421 ctcggcgtgg tgatcggcaa gcccgcgaaa tatatcgacg aagccaacgc actcgattac
      481 gtcgccggct actgcgtgat caacgacgtg tccgaacgcg aatggcagat cgaacgcggc
      541 ggcacgtggg acaagggcaa gggcttcgat acgttcggcc cgatcggccc gtgggtcgtg
      601 acgcgcgatg aagttgccga tccgcagaat ctgagcctgt ggctcgaagt ggacggccac
      661 cgctatcaga acggcagcac gaagacgatg gtgttcggcg tcgcgaagct ggtgtcgtat
      721 gtgtcgcagt gcatgagcct gcaaccgggc gacgtgattt cgaccggcac gccgccgggc
      781 gtcggtatgg gtgtgaaacc gaatccggtg tatctgaagc cgggccagac gatccgtctg
      841 ggtgtcgaag gtctcggcga gcagacgcag aagacttacg cggcggagtg agttgatcgt
      901 ttagctgaca tgtagtgttg atgtgagccg ttagatgtga agaggccgtt cccgcgcaat
      961 gcaggaacgg ccttttttta tcgaaccctc agtcggcgcc cggatgcctc aattcgcgac
     1021 cttttgcccg aagagcgacc gcatgatcag cgcgcgacct tggaagacgt gcccggcttc
     1081 cgtacaccat gaccatgcgc tgacaagaaa cgctttcacc cgttcaaccc gccggttcgt
     1141 gctcgcccat atcgccgatc cgattgatcg tgccttgggc aaccgcgacg agcttctcac
     1201 gaccgtcgtc catcacaaac acgttgcact ggcaggttgc ctgggaccgg cccgtgtaga
     1261 cgaccctcgc acgcgcgatc agcgtgccgt tcaccaccgg ccgcagatag ttgatcttgt
     1321 actcgctggt gacgactttc ggacccagcg acagcgcgcc ggcgaaagtc agcgcattgt
     1381 cggcgagata actgatgacg ccgccatgca cgaagccacg ctgttgccgc agctcgtcgc
     1441 ggatcggcag gcagagcgtc aattcgtcgg tgcccgtgtg catcagctcg gcgcctaaca
     1501 gcatgctgaa cggctgggcg tgcagtgcgc cgcgcgcccg gtcgaggagg tcggtcattg
     1561 tggttccttc ggggcggtgg cccgtggcga tatctgcact ggcgtgcgat gtgcagtccc
     1621 tacccactct aggaagcgct ggcgcaccgc gtcaaggtca atcggaaata tgctcagggc
     1681 attgttgcgc caaatgattg gaattgcgga gttgggcaat gaaacggggt tgtaagacga
     1741 tctttaacga aattagccct caaggagcgc gcgccgatgc cgttaaccct gatatggctc
     1801 cggtcattct ttgcagcctg gcagcgcgca gtcggcgaag gagatcaaga cgctgagcag
     1861 cgaatcgatg gccgaggttc aaggcggttc ggtgctggtc gagcatgcag cggcatgcct
     1921 cactgcatca acagacgcaa caactgaaag aggccgtttc cgtgatcgaa atttcggaaa
     1981 ctgtgttgcg aacgcaacaa atggacgaca cgcgagggca agcggcggcg ttcgcgctgt
     2041 cggggatgcg ggcaatttga accgcacagg cgcctggaaa aagggcatct gccaaagaaa
     2101 atggcccgcg aaagcgggcc attttgcatt agacgcaagc gcctaagggt gcctcaaagt
     2161 acctgaaggt acttaggccg gctggatgtt tgcagcctgc ttgcccttcg ggccttgctt
     2221 gacttcgaac gtcactttct ggttttcctg cagggacttg aagccgctgc cttgaacttc
     2281 cgaaaagtga gcaaacaggt cttcgccgcc gtcgtccggg gtgatgaagc caaagccctt
     2341 tgcatcgtta aaccacttca cagtacctgt tgccattttt aatccagtaa atgttgtgtg
     2401 ttgcattcgc gaccgctatt tctcaacacg agatcgagcg cgaagtacga gttgtatcac
     2461 gtctttatga tggacgccaa ccaacaagta ttcgggtatt cccctgattg ggccgttttg
     2521 ggaccttttt cgggcccaga acgcattttg cggggtgttc cggccggtag ggtaaactgc
     2581 ttgtattact cttccaggaa aaacattgac acattatttg caagaagaac ttacctcgtt
     2641 tttgagtcgt ttgaccccct atctggcgat attggagtct aaatcacctc aagaaggcgc
     2701 cccggacccg gtcggccgta tcacggtcga cagcgaccat caccaggctt tcgcccttca
     2761 cactaccgac aaaaagccgc acggcgacac atcggaaacc cctaccggac aaagcgcgac
     2821 ctacacgcga tttaaaactg ccgacgccga ctggggacct tggcaacgcg aagaatattg
     2881 attgaccggc cagtcggctt ttaacgccga tgtccgcgag atggctgctg gtgcctagcc
     2941 cggcaacgcc tcgtaggcgg tggcgaggtg atagggcgtg gtcgacggca tgtcggcacg
     3001 cgccacgcca ccagccgccg ttttgcattc gaaccagcct tgcggacgca gaaagcgttg
     3061 ccggaaaagc tcgatttgcc gcggcagcgc ggcgagcgca gcagggtcgt ggtggcttgc
     3121 caatgcgcgc agatattctg tctgagccca gattcgctgg gtggcgtcct tgatccgtcc
     3181 cgtttcgtcg agcgaggcgc acacgccgcc tgtttgcggg tccacgccat attgctgcgc
     3241 gaaactgaac gcgcgcgtca acgactcgtc gagacccgag gcttcgaaca agctgcccgc
     3301 ctgcttcacc agccagaacc attcgaactg gtgaccgggt tccagacggt tgtcgtccgc
     3361 gccaatcggc aattcggcga tgcaaccgct cggcgcatgt acaaaatgtc gcgcaatggc
     3421 gctggcgaga cggcgcaagg ccgcgtcgaa ggcgttgtcg tgcgtggcag cgcgcgcggc
     3481 cagccaggct tccgtcagat gcatgagggg attctggatc ggtgtgccga ccaccttggc
     3541 gaagtcggcg gtgagcgcgg cgttgaacag gtcaccttcg gccgcgaact gcgactgaat
     3601 caatgccgac gtgcgatgca cgacttccag cgcgtcgcgg ttgccggaac ggctgccgta
     3661 ttccgcgcac gcgaacacca cgaatgcatg cgtgtacagg tctttggtcg tgtcgagcgg
     3721 ggcgccctgt gcatccacgc tatagaacca tccgccgtgg cgcgggtcct gaaaggtgtg
     3781 catcagtgaa tcgaacagca cctgggcgtg ggccgcatcg tctgcttgcg agaacacgaa
     3841 caattgtcgc gcgcacgcca tcgcccggta gcgttgcgcg ggcaacggct ccgaaccctc
     3901 ggcgctgacc gcttcgtagg gtagtttcag cgcggggtta aaaccgggcc cgcgccacag
     3961 cggcagcacc acacgagtga agtgatcacg caatgcggtg gcgagtgtgc tcgtcgaagc
     4021 ggtggaaggg gtcatgctct gcattagttc ggttggtcaa acgcggtcat tcgaaacgcc
     4081 tgcggacttt ggctcgcgcc gtggcgcggc cgccggcgat catgctgagt cagcgattat
     4141 ggatgctgat cggcctgtaa caggaagcct cgcaagcagc atgtcgatcg tcccggccag
     4201 ccgcgcggcg gcgtgcggcc aagcataaag caaacggcta ccgctggcac tgcgccgcag
     4261 gtgcgcaata cgcttcaaat aatttcagat aaggaggaaa aacgatgttg aagtcgtgat
     4321 cacgctgcat cgggtgtcga acaccgaata tgaggcgatc tgcgcgcgct tgcgtcagct
     4381 gcctggcgtc aggcggttcc ggcaggacgg ggcggcgtct taaaaagcgg aaaatcgtcg
     4441 gcgaatggtt tcggcagggg cgggcggcca tgcgacaatg cggtctcgaa aaattccaat
     4501 ggcattgacg agcggtgccc ggcgcccgct ttccggcgac gaccgctttt ttctctatgg
     4561 cagattccgt agcatccacg cagtcccggc ctcagcgtgc cgcttcgaag aagcggcgtc
     4621 aggccaccgt ctcgaccgaa actgaaaaca agctggcgcg cgccgcgcgc tcggcacaac
     4681 gtctcgcgca actgagcgat gcctcacgcg acgacagcac actcgacctg ttccccgacg
     4741 accccacccg cgccacgctg gaagcgatga atatcgacgt ccggcaaggc acgctgcatg
     4801 gctttgaatt acccgatgtc gtgttggcgg cggtcggcgc gatcgatgcc gatggcgcgt
     4861 cgtttgaggc acggccggcg cggcgcgcgc ctcgcgctgc gaaatccgat gaaccg
//
LOCUS       DATASH010000729         9682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3678687,
            whole genome shotgun sequence.
ACCESSION   DATASH010000729 DATASH010000000
VERSION     DATASH010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 9682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9682
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3678687"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VHV99_19245"
     CDS             <1..424
                     /locus_tag="VHV99_19245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3637581.1"
                     /translation="AVIDTNLTSLFNVTKQVIDGMVERGFGRVINISSVNGQKGQFGQ
                     TNYSTAKAGIHGFTMSLAQEVATKGVTVNTVSPGYIGTDMVKSIRPDVLEKIVATIPV
                     RRLGQPGEIGSIVAWLASDESGFSTGADFSLNGGLHMG"
     gene            559..1176
                     /gene="phaR"
                     /locus_tag="VHV99_19250"
     CDS             559..1176
                     /gene="phaR"
                     /locus_tag="VHV99_19250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HEX3637582.1"
                     /translation="MTTTTKKTAERLIKKYPNRRLYDTETSTYITLTDVKQLVLDQED
                     FKVIDAKSNEDLTRAILLQIILEEESGGLPMFSSSMLSQIIRFYGHAMQGMMGTYLEK
                     NIQAFIDIQAKLADQSKNLYEGKAMNPEVWSQFMNMQAPMMQGMMTSYIEQSKNMFVQ
                     MQEQMQNQAKSMFATFPFTPGGPVNAAGVPVTPANPQTNPEPEKK"
     gene            1574..2626
                     /locus_tag="VHV99_19255"
     CDS             1574..2626
                     /locus_tag="VHV99_19255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEX3637583.1"
                     /translation="MSQLFLAPMEGLADYVLRDVLTSTGGFDGCVSEFVRVTGSVLPE
                     RVYERDTPEVLHGGRTPGGTPMVIQLLGSDPEWMALNAAQAASLSPHGLDLNFGCPAK
                     VVNRHGGGAMLLAHPEQLNRIVSSVRAAVPGGIAVTAKMRLGVSDTSLAIECATALAE
                     GGAASLVVHARTRDHGYRPPAHWEWIARIDAAVDVPVIANGEVWSVADWERCRAVSGC
                     ADVMVGRGAVSDPFLALRIRGLMDRTPSGAEWPAVLSHLADYLKKLQARVAARHEHGR
                     VKMWLSYLKRTWPQAAELHAAIRRLHDSREILEVIEHALAFNGVTPAHRSAIPDLPDP
                     ATPHHLAGCMVAGAVQ"
     gene            2741..4132
                     /gene="rimO"
                     /locus_tag="VHV99_19260"
     CDS             2741..4132
                     /gene="rimO"
                     /locus_tag="VHV99_19260"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEX3637584.1"
                     /translation="MSQTISPAAPISSTPKVGFVSLGCPKALVDSEQIITQLRAEGYE
                     ISGTYDGADLVVVNTCGFIDEAVQESLDAIGEALSENGKVIVTGCLGAKQSASGSNLI
                     EEVHPKVLAVTGPHALGEVMQAVHNHLPKPHDPFIDLVPAAGVKLTPRHYAYLKISEG
                     CNHRCTFCIIPSMRGDLVSRPVAEVMLEAENLFKSGVKELLVISQDTSAYGVDVKYRT
                     GFWNGKPIKTRMTDLVGALGELAAQYGAWVRLHYVYPYPSVDEVIPMMAEGPFKGHVL
                     PYLDVPFQHAHPEVLKRMKRPANAEKVMERVKAWREICPDLTIRSTFIAGFPGETEEQ
                     FQTLLDFIREAELDRVGCFAYSPVEGASANELDGALPDEVREERRARFMEVAEEVSAK
                     RIAKKVGKTLKVLVDEINADGGIGRTAADAPEIDGVVYIAPATKASKRYKVGDFVSVK
                     ITGADGHDLWGEV"
     gene            4286..5191
                     /locus_tag="VHV99_19265"
     CDS             4286..5191
                     /locus_tag="VHV99_19265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEX3637585.1"
                     /translation="MLALGEAMIEFNQAVKDQPNYLQGFGGDTSNFCIAAARQGAKTG
                     FVSAVGSDHFGRLLIDLWEREQVETSLVRIDPHASTGVYFVSHGPAGHAFDYLRAGSA
                     ASHYAPHDLPLDAIAAAKVIHLSGISLAISLSACDAALAAIAHARANGVRVSFDTNLR
                     LKLWPLARARAVMLEAIRQTDMCLPSWDDVTELTGLTGRDEIVDFLLSHGPRVVALKL
                     GKEGSYIATPDERRVVPGHVVNAVDATGAGDCFGGAFIARIVAGDDPFQAARYANVAA
                     ALSTQGYGAVAPIPSHATVEHLLAG"
     gene            5271..6455
                     /gene="bktB"
                     /locus_tag="VHV99_19270"
     CDS             5271..6455
                     /gene="bktB"
                     /locus_tag="VHV99_19270"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004521353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketothiolase BktB"
                     /protein_id="HEX3637586.1"
                     /translation="MQRDVVVVSGVRTAIGGFGGSLKDFPPTDLGARVVREALARANV
                     SGDEVGHVVFGNVVHTEPKDMYLARVAAINGGVAQHAPALTVNRLCGSGLQAIVSAAQ
                     SVLLGDADIAIGGGAENMSRAPYSMPAARFGQRMGDARLVDMMVGALNDPFQSIHMGV
                     TAENVARKYEISRETQDALALESHRRAANAISAGYFKDQILPITIPSKKGDVVFDTDE
                     HARMNATAEDFSKLKPVFAKENGTVTAGNASGINDAAAAVVLMERSVAEKRGIKPLAR
                     LVSYAHAGVDPAYMGIGPVPATRKALERAGLTVADLDVIEANEAFAAQACAVSKELGF
                     DPAKVNPNGSGISLGHPIGATGALITVKALYELQRVGGRYALVTMCIGGGQGIAAIFE
                     RI"
     gene            6659..7318
                     /locus_tag="VHV99_19275"
     CDS             6659..7318
                     /locus_tag="VHV99_19275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637587.1"
                     /translation="MQESNAIKGAMRRRAWAVSQGGFLRSVVSVAALLCAGAGAVLPA
                     QAWAESDAVKELAAPPPIQLPLKPSPEFAKFPRYAGMLGSRQIVLRLGAKTDDPAGVH
                     GEYQYTDTGQVILIAGDRDGDTLEVEESNDGTHITGNWVGKFAADGSVAGDRMNVDDS
                     DPQPFDLKPLAAGQAVPAAVNKAATAPQASAAAGPAVPASSPAAGGHAVGGVSNLQTG
                     E"
     gene            7458..8642
                     /locus_tag="VHV99_19280"
     CDS             7458..8642
                     /locus_tag="VHV99_19280"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEX3637588.1"
                     /translation="MTQSKLKRGLQTQIVRAEDQLTPGFESFSMPVTRASTVVFPDLA
                     TMRALDWKNDAQWRYGLHATPTSLALAQRLATIEGGHHALLQPSGLSSISNVYFGLVK
                     SGDDVLIPDNVYSPNRDHGDWLARDFGITARYYDPMIGAGIADLIQPNTRLIWLEAPG
                     SVTMEVPDVPAITAVARARNVVTAIDNTWSAGLGFRPFEHGVDISVQALTKYQSGGGD
                     VLMGATITVDRELHLKLKAARMRMGIGVSSDDCSLILRSLPSMQLRFDAHDRAALGLA
                     TWLKTRPEIAAVLHPALSDCPGHEFYKRDFTGAGGLFSVVFDERYSAAQIDTFCESLE
                     LFSLGWSWGGAHSLAMPYDVAAMRTAGQWPYRGTLVRFYIGLEDEADLRADIEQRLVA
                     LG"
     gene            8822..9268
                     /locus_tag="VHV99_19285"
     CDS             8822..9268
                     /locus_tag="VHV99_19285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637589.1"
                     /translation="MNLFWMLLNLGVPIVGPIFTLALAAPTHGWRVAKALIAASVKDG
                     QLFWCAIGLCAAAVYEAVTALEVGRDGMPILEGAIAGFCMLAFACSSIVMLSLVNAHH
                     AEASSCIRDGQKWLDTGALSRTAIVISILATSLAAVLFVILHVHLN"
     gene            9318..9449
                     /locus_tag="VHV99_19290"
     CDS             9318..9449
                     /locus_tag="VHV99_19290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637590.1"
                     /translation="MDKLFTYLQSDENYARVLKWHGAVIIITGISMLAMFMFADFKR"
     gene            complement(9501..>9682)
                     /locus_tag="VHV99_19295"
     CDS             complement(9501..>9682)
                     /locus_tag="VHV99_19295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEX3637591.1"
                     /translation="CAQLGIEPTRAIAMGDGSNDLKMMAAAGLSVAFRAKPVVREAAS
                     VAFNYVGLDGLLRLF"
BASE COUNT         1779 a         2922 c         3171 g         1810 t
ORIGIN      
        1 ggcggtgatc gacaccaacc tgaccagcct gttcaacgtc accaagcagg tgatcgacgg
       61 catggtcgag cgcggcttcg gacgcgtcat caacatctcg tcggtgaacg gccagaaggg
      121 gcaattcggc cagaccaact attcgaccgc gaaggccggc attcacggct tcacgatgtc
      181 gctggcgcag gaagtggcca ctaagggcgt gacggtcaac accgtgtcgc caggctacat
      241 cggcacggat atggtcaagt cgatccgccc ggacgtgctg gaaaagatcg ttgcgacgat
      301 tccggtgcgc cgtctcggcc agccgggcga aatcggctcg atcgtggcat ggctcgcctc
      361 ggacgaatcg ggtttctcca cgggcgcgga tttttcgctg aatggcggtt tgcatatggg
      421 ctgagtcatc ggcgcgccgc tcacaaggcg gcgtgcttct gctcagatgg ttccggcggc
      481 gtctcgcctg cgcaattgcc cgattgtgca ggcggcatga cgccgtttcc gcgcttaact
      541 cgcgctcaaa ggcgttacat gaccactact acaaagaaaa cagccgaacg attgatcaag
      601 aagtacccga atcgtcgact ctacgatacc gagacaagca cgtacatcac gctgacggat
      661 gttaagcagc tcgtgttgga tcaggaggat ttcaaggtca tcgatgcgaa gagcaacgag
      721 gacctgacgc gtgccatcct gttgcagatc attcttgaag aggagagcgg cggcttgccg
      781 atgttctcgt cgtcgatgtt gtcgcagatc atccgttttt atggccacgc gatgcagggc
      841 atgatgggca cgtatctgga gaagaatatc caggctttca tcgacattca ggcaaagctc
      901 gccgaccagt cgaagaacct gtatgaaggc aaggcgatga accccgaagt ctggtcgcaa
      961 ttcatgaaca tgcaggcgcc gatgatgcaa ggcatgatga ccagctatat cgagcagtcg
     1021 aagaacatgt tcgtgcagat gcaggagcag atgcagaacc aggcgaaatc gatgttcgct
     1081 actttcccgt tcaccccggg tggaccagtg aatgctgcag gagtaccggt cacgccagct
     1141 aatccgcaaa ccaatccgga gccggagaag aagtagcggc gcagagggat gctgacgccg
     1201 gcgcgcttag cgcgtctctg cgtggcggtc ccgtggccgc ggcggacggt gggcacgtcg
     1261 cgccgagggt gccgggtgtg gtcgggtggc tcgcgagcgg agcgcgaggg gatagccaag
     1321 ggcgcggtac aatcgcgggt tgcgtgcggc gtcgctgtgg cggggtgtca gcccatggtc
     1381 ttcatgaccc tcgtgatcct gccacctttc ggcctcgtcg cgccgttccc gtttgttgcc
     1441 accgctatcc acctgcccac ctatgtcgca aactcgtttt cctagcgtgt tcatgccttc
     1501 gattccgagg gtcggattta tcagccttgg ctgaccggaa gattcgcaaa gacagcgctt
     1561 gcgccgtatc cacatgagtc aactgtttct cgcgcccatg gaagggcttg cggactacgt
     1621 gctgcgcgac gtgctgacca gtaccggcgg attcgacggg tgcgtctccg agtttgtccg
     1681 ggtgacgggc tcggtgctgc ccgagcgtgt ctacgaaaga gatacgcccg aagtccttca
     1741 cggaggccgt acaccgggcg gcacgccgat ggtgattcaa ctgctcggca gcgatccgga
     1801 atggatggcg ctgaatgcgg cccaggcggc cagtttgtcg ccgcatggac tcgatctgaa
     1861 cttcggttgt cccgccaaag tcgtcaaccg gcacggtggc ggcgcgatgt tgctggcgca
     1921 ccctgaacag ctgaaccgga tcgtttcgtc cgttcgcgcg gcggtgcccg gcggcatcgc
     1981 ggtgacagca aaaatgcggc tcggcgtgtc cgacacttcg cttgcgatcg aatgcgcgac
     2041 agcgctggcg gaaggtggcg cggcctccct cgtcgtccat gccagaacgc gtgaccatgg
     2101 ctaccggcca ccggcgcact gggagtggat cgcgcgtatc gacgccgccg tggacgtgcc
     2161 ggtcatcgcc aacggagagg tctggagcgt ggccgactgg gagcggtgcc gtgcggtcag
     2221 cggctgcgcg gacgtgatgg tcgggcgcgg cgccgtgtcg gatcctttcc tcgccttgcg
     2281 gatacgcgga ctgatggacc ggacgccttc cggcgcggaa tggccggccg tgctgagtca
     2341 tctcgccgac tacctgaaaa aactgcaagc ccgtgtcgcg gcccgccatg agcacggacg
     2401 cgtcaaaatg tggctgagct atctgaagcg gacctggccg caagccgccg aactgcatgc
     2461 agccatccgg cgcctgcatg attcgcgtga gattctcgag gtgatcgagc acgccttggc
     2521 gttcaatggt gtaacgccgg ctcaccgaag tgcgattccc gacctgcccg atccggcaac
     2581 gcctcaccac ctggccggtt gcatggtcgc cggcgcggta caataaacgg ttacgcgcgc
     2641 gggacgttgt ccgggccgcg gtgtctgtca ccatcgactc tgcgctgccg caccgtcccc
     2701 ctttgtcgcc attgtcaccg ctatatgccg gaccccacct atgtcgcaga ccatttcccc
     2761 agccgcgccg atctcttcca cgccgaaggt cggtttcgtc agcctcggct gccctaaagc
     2821 actcgtcgac tccgaacaga tcatcacgca actgcgcgcc gagggctacg aaatctccgg
     2881 cacctacgac ggcgcggacc tcgtggtcgt caacacctgc ggcttcatcg acgaagcagt
     2941 gcaggaaagc ctcgacgcga tcggcgaagc gttgagcgaa aacggcaagg taatcgtcac
     3001 cggctgcctc ggtgccaagc agagtgcgag tggctctaac ctgatcgaag aagtgcatcc
     3061 gaaggtgctg gccgtgaccg gcccgcacgc gctcggcgaa gtgatgcagg cggtgcacaa
     3121 tcatctgccg aagccgcacg atccgttcat cgatctggtg ccggctgcgg gcgtgaagct
     3181 cacgccgcgc cactacgcgt acctgaaaat ttccgaaggc tgcaatcacc gctgcacgtt
     3241 ctgcatcatc ccgtcgatgc gcggcgacct cgtgtcgcgc ccggtcgccg aagtgatgct
     3301 cgaagcggag aacctgttca agtccggcgt gaaggaactg ctggtgattt cgcaggacac
     3361 gagcgcatac ggcgtcgacg tcaaataccg cacggggttc tggaacggca agccgatcaa
     3421 gacgcgcatg accgatctgg tcggcgcgct cggcgagctc gcggcgcaat acggcgcatg
     3481 ggtgcgtttg cactacgtgt acccctatcc gagcgtcgac gaagtcatcc cgatgatggc
     3541 cgaaggtccg ttcaagggcc atgtgctgcc gtacctcgac gtgccgttcc agcacgcgca
     3601 tccggaggtg ttgaagcgca tgaagcgtcc ggccaacgcc gagaaggtga tggagcgcgt
     3661 gaaggcatgg cgtgaaatat gccccgatct gacgatccgc agcaccttca tcgccggctt
     3721 cccgggcgag accgaagagc aattccagac gctgctcgat ttcatccgcg aggcggaact
     3781 ggatcgggtc ggctgctttg cttactcgcc ggtcgaaggc gcgagcgcca acgaactcga
     3841 cggcgcgttg cccgacgaag tgcgtgaaga acgccgcgcg cgtttcatgg aagtggccga
     3901 agaagtatcg gccaaacgga tcgcgaagaa ggtcggcaag acgctgaaag tgctggtcga
     3961 cgagatcaat gccgacggcg gcatcggccg tacggccgcg gatgcgcccg agatcgacgg
     4021 tgtcgtgtat atcgcgccgg ccaccaaggc gtcaaaacgc tacaaggtcg gcgatttcgt
     4081 ctcggtgaag atcaccggcg ccgacggtca cgatctgtgg ggcgaggttt aagcgatgac
     4141 ggggagtatg gctggcacgc ctggaggcct gaccccggaa accgcgcgcc cgggtgcccg
     4201 agacccggat ggccgaatcc cggatgcccg gcgcccggat gcccggcgcc cggatgtccg
     4261 gcccccgggt gcccggcgcc ctgaaatcct cgcgctcggc gaggcaatga tcgagttcaa
     4321 ccaggcggtc aaagaccagc cgaactatct gcaaggtttc ggcggtgaca cgtcgaactt
     4381 ctgcatcgcg gcagcacggc agggcgcgaa gaccggtttt gtgtcggctg tcggcagcga
     4441 ccatttcggg cgtctgttga tcgatctgtg ggagcgcgag caggtcgaga cgtcgctcgt
     4501 gcggatcgat ccgcatgcgt ccaccggcgt ctatttcgtt tcgcacggtc cagcaggcca
     4561 tgcgttcgac tatctgcgcg cgggttcggc cgcaagccac tacgcgccgc acgatctgcc
     4621 gctcgatgcg atcgccgcgg ccaaggtcat ccacctgtcc ggcatcagcc tcgcgatcag
     4681 tttgagcgcc tgcgacgcag cgttggcggc gatcgcgcat gcgcgcgcga acggcgtgcg
     4741 cgtgagtttc gacaccaatc tgcgcctgaa gctgtggccg ctcgcgcggg cgcgcgcggt
     4801 gatgctcgag gccattcgcc aaaccgacat gtgcctgccg agctgggacg atgtcaccga
     4861 actcaccggc ttgaccggcc gtgacgagat cgtcgatttt ctgttgtcgc acggtccgcg
     4921 tgtcgtcgcg ctcaagctcg gcaaggaagg ctcgtacatc gccacgcctg acgagcggcg
     4981 cgtcgtgccg ggccacgtcg tcaatgcggt cgatgcgacc ggcgcgggcg actgtttcgg
     5041 cggcgcgttc atcgcgcgta tcgtcgcggg tgacgatcct tttcaagcgg cgcgttatgc
     5101 gaacgtcgcc gcggcactgt ccacgcaagg ttacggcgcg gtcgcgccga tcccttcgca
     5161 cgcgacggtc gaacaccttc tggcgggctg atcacgaggc gctgggacac gtggggcgcg
     5221 cgggccgcca tggcaagatc atcttgagaa actaaagaga ggagcgagcg atgcaacggg
     5281 acgtagtggt ggtaagcggt gtacgtacgg cgatcggtgg ttttggcggc agtctcaagg
     5341 attttccgcc cacggatctg ggtgcgcgcg tcgtgcgtga ggcactggcg cgcgcgaacg
     5401 tgtcgggcga cgaagtcggc catgtggtat tcggcaacgt cgtgcatacc gaaccgaaag
     5461 atatgtatct ggcgcgcgtg gccgcgatca atggcggcgt cgcgcaacac gcgcccgcgt
     5521 tgaccgtcaa ccgcctgtgc ggttcgggct tgcaggccat cgtttcggcc gcgcaaagcg
     5581 tactgctcgg cgacgctgat atcgcgatcg gcggcggagc ggaaaacatg agccgcgcgc
     5641 cgtattccat gccggccgcg cgcttcggtc agcgcatggg cgatgcgcgc cttgtcgaca
     5701 tgatggtcgg cgcgctgaac gacccgttcc agtcgatcca tatgggcgtg accgccgaga
     5761 acgtcgcgcg caaatacgag atctcgcgcg aaacgcagga tgcgctcgcg ctcgaatcgc
     5821 atcgccgcgc ggccaacgcg atttccgccg gctacttcaa ggaccagatc ctgccgatca
     5881 cgattccgtc gaagaagggc gacgtcgtgt tcgatacgga tgaacacgcg cgcatgaacg
     5941 cgacggctga agacttctcg aagttgaagc cggtgttcgc gaaggaaaac ggcacggtga
     6001 ctgcggggaa cgcgtcgggc atcaacgacg cggcggcggc ggtcgtgctg atggagcgca
     6061 gcgtcgctga aaagcgcggc atcaagccgc tggcacggct ggtttcgtac gcgcacgcag
     6121 gcgtcgatcc ggcgtacatg ggcatcggcc cggtgcccgc cacgcgcaag gcgctagaac
     6181 gcgccggcct gacggtcgcc gatctggacg tgatcgaagc gaacgaggcg tttgcggcgc
     6241 aggcgtgcgc ggtcagcaag gaactcggtt tcgatccggc caaggtcaat ccgaacggct
     6301 cgggcatttc gctcggccac ccgatcggcg cgaccggcgc actgatcacc gtcaaggcgt
     6361 tgtatgaatt gcagcgggtc ggcggccgct atgcgctcgt gacgatgtgt atcggcggcg
     6421 ggcaaggcat cgcggcgatc ttcgaacgga tctgaggctg ggtgggggct gaggctgaag
     6481 ttgatgctga ggctaaagct gatgctcagg ttgaggctga ggctaaggct gaggcttgat
     6541 gctaaggcgc agcaccgcgg cgcagcatca agcctcgatt ctccccgccg aacgaaatcc
     6601 gacaccatgc ggatagcaaa ccagtatcga gcggcaccag gtaacaagga aagaacggat
     6661 gcaggaatcg aatgcgatca agggtgcgat gcgtcggcgt gcttgggcag tctcccaggg
     6721 gggcttcctt cggagcgtag tgagcgtggc cgcattgctg tgcgccggcg ccggggcggt
     6781 attgcccgcg caggcgtggg cggaaagcga cgcagtgaag gagttggcgg caccgccgcc
     6841 gattcagttg ccgcttaagc cgagccccga attcgcgaag tttccgcgct atgccggcat
     6901 gctcggctcg cgtcagatcg tgctgcggct cggcgcgaaa accgacgacc ccgccggtgt
     6961 gcacggcgag tatcaataca ccgataccgg tcaggtaatc ctgatcgcgg gagaccgcga
     7021 cggcgacacg ctcgaagtcg aggaatcgaa cgacggcacg catatcaccg gcaactgggt
     7081 cggcaagttt gccgccgacg gttcggtggc gggcgaccgg atgaacgtcg acgattccga
     7141 cccgcagccg ttcgatctga aaccgctggc agcggggcag gccgtgccgg ctgccgtgaa
     7201 caaggcggca acggctccgc aggcgtcggc agccgcaggt ccggctgtgc cggcttcgtc
     7261 gcccgccgcg ggcggccacg ccgtcggcgg ggtcagcaac ctgcaaaccg gcgagtgaag
     7321 cgctgcctcg cgcctgacgg cttgcacgct ctgccaggcg caactctgcc ggctcgtcta
     7381 ctcttgcgtg tgcggcgccg tccggaacag cgcccttggt tttctgtctc ctgcctagtc
     7441 actcaccgaa tttcaccatg acccaatcca aactcaaacg cggcctgcaa acccagatcg
     7501 tgcgtgccga ggaccagctc acgccgggct tcgaatcatt ctcgatgccg gtgacgcgcg
     7561 cttcgacggt cgtgttcccg gatctcgcga ccatgcgcgc gctcgactgg aaaaacgacg
     7621 cgcaatggcg ctatggcctg cacgccacgc cgacttcgct cgcgctggcg cagcggcttg
     7681 ccacgatcga aggcggccat catgcgttgt tgcagccttc ggggttgtcg tctatttcga
     7741 atgtgtactt cggccttgtg aagtccggcg acgacgtgct cattcccgat aacgtctact
     7801 cgccgaaccg cgatcatggc gactggctgg cgcgcgattt cggtatcacc gcgcgctact
     7861 acgatccgat gatcggcgcg gggatcgcgg atctgatcca gccgaacaca cggttgatct
     7921 ggctcgaagc gcctggctcg gtcacgatgg aagtgcccga cgtgcctgct atcaccgcgg
     7981 tggcgcgcgc ccgcaacgtc gtcacggcga tcgacaatac ctggtccgca gggctcggtt
     8041 tccggccgtt cgagcacggc gtggatatct cggtgcaggc cttgaccaag taccagtcgg
     8101 gcggcggcga cgtgttgatg ggcgcgacga tcaccgtcga tcgcgaattg catctgaagc
     8161 tgaaggcggc gcgcatgcgc atggggatcg gcgtgtcgtc ggatgactgt tcgctgattc
     8221 tgcgcagcct gccgagcatg caactgcgct tcgacgcgca tgaccgtgcg gcgcttggcc
     8281 tcgccacgtg gctgaagacg cgtccggaaa ttgccgcggt cttgcacccg gcgttgtcgg
     8341 actgtccggg acatgagttt tacaagcgcg actttacagg cgcgggcgga cttttctcgg
     8401 tcgtgttcga cgagcgttat agtgcggcgc aaatcgacac gttctgcgag tcgctcgagc
     8461 tgttttcgct cggctggagc tggggcggcg cgcacagtct tgcgatgccc tacgacgtcg
     8521 ccgcgatgcg caccgcaggg caatggccgt atcgcggtac gctggtgcgg ttctacatcg
     8581 gcctggaaga cgaggccgat ctgcgcgcgg atatcgaaca gcggctggtg gcgctgggtt
     8641 aagcgcctga acgcaggtgg ttgttggtgg aaaaagccca tcgggacgcg ttcccgatgg
     8701 gctttttatt ttgtcaagat agccagtaag cctaaccatt cggctgaatg gtcgacgccg
     8761 gatctcgacg ctacgatggt gcgcaccaca actaacaatt accgcacgat ggtgagcttc
     8821 gatgaatcta ttctggatgt tgctgaatct tggcgtgccg attgtcggcc cgatctttac
     8881 gctcgcgctc gccgcaccca cgcacggctg gcgcgtcgcg aaggcgttga tcgccgcatc
     8941 ggtgaaagac ggccaactgt tctggtgtgc gattggattg tgcgcggccg cggtttatga
     9001 ggcggtgact gcgctggagg tggggcgcga cggaatgcct atcctggagg gcgccattgc
     9061 tggattttgc atgctggcat tcgcctgttc gagcatcgtg atgttgtctt tggtcaatgc
     9121 acatcatgcg gaggcaagct catgcattcg tgacgggcag aagtggcttg atacgggggc
     9181 gctgtcgcgc acggcaatcg tgatttcgat tttggcaaca tctcttgctg ctgtactgtt
     9241 tgtaatccta catgtgcacc tgaattagat ttttagataa cgcagctttt atactgatct
     9301 acttttggga ggcgctaatg gacaaattat tcacgtatct gcaatctgac gaaaactacg
     9361 ctcgtgttct gaagtggcac ggcgcggtga tcatcatcac tgggatcagc atgctggcca
     9421 tgttcatgtt cgcggacttc aaaagatagt gttcctgcac gcgctggccg ccactgcatc
     9481 gggccagcgc cactcaagct tcagaacaga cgcagcaacc catccagccc aacgtagttg
     9541 aacgccacgc tggccgcttc gcgcaccacc ggcttcgcgc ggaatgcaac ggaaagtccc
     9601 gcggcggcca tcattttcag atcgttcgat ccatcgccca tcgcaatcgc gcgcgtcggc
     9661 tcgataccga gttgagcgca tg
//
LOCUS       DATASH010000730         4164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4670204,
            whole genome shotgun sequence.
ACCESSION   DATASH010000730 DATASH010000000
VERSION     DATASH010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4164
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4670204"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VHV99_19300"
     CDS             complement(<1..437)
                     /locus_tag="VHV99_19300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HEX3637592.1"
                     /translation="MSIRYPLLNRELGILGFNERVLAQAADPSVPLLERLRFICITSS
                     NLDEFFEVRMAGLQEQMRDNPGALSPDGMSLQHVYDLVVERAQRLVHRQYTMLHDTVL
                     TALEAEGIYFHGTEAWNEAQTEWARNYFFDELLPVLTPIGLDPA"
     gene            640..2265
                     /gene="ppx"
                     /locus_tag="VHV99_19305"
     CDS             640..2265
                     /gene="ppx"
                     /locus_tag="VHV99_19305"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEX3637593.1"
                     /translation="MASLISAAGHKAPKASSGHPAPSASTAAALARMESAYSMVTTPQ
                     LLAAVDLGSNSFRLIVGRVEETDAGSQIYQVDALREPVRLAAGLSRDKMLDRASQVRG
                     WDALKRFGERLRDFHPDHVRAVATNTLRIAKNANEFLGEAQAALGFPIEVIAGREEAR
                     LIYAGAAHSVPASAGKRLVVDIGGGSTEFIIGSHYTPIKMESLYIGCVSHSRAFFPAG
                     NVDEYTMRQAELAAGREIQIISAEYKKTGWEQAIGSSGTARALAELVEANGFNDPGIT
                     HGISRGGLERLKRALIKAENVNRLKLIALKSDRIPVLAGGLSIMIAVFDELGVDYVDT
                     TDGALRLGVLYDLLGRSQHEDMRTVTAEGFMRRYGVDRAQAGRIGELAVRFYDQFVEP
                     DEERRVENRMFLGWAAALHEIGLSISHSAYHKHSAYIASNADMPGFSRTDQARLAALV
                     LGHAGKLGKLSQTREVEWPLLFCLRLAALLCRRRADVGLPGIAVTQVNGGYEVRLPNE
                     WVANNPLTDYSLIQEAAEWEKVGISYRVVYTDD"
     gene            complement(2428..3240)
                     /locus_tag="VHV99_19310"
     CDS             complement(2428..3240)
                     /locus_tag="VHV99_19310"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004266561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HEX3637594.1"
                     /translation="MRELPTPTLPLASIFETRRLPRAEETVTAQHRLQVTWARTDEEL
                     REAQRLRYRVFADEMGARLTGPAGLDVDSFDSYCDHLLVRDLDTLKVVGTYRALPPHQ
                     AARIGRLYAESEFDVSRLTHLRAKMVEVGRSCVHPDYRSGAVIMSLWAGLAAYMKHNG
                     YETMLGCASVAMVDGGHYAANLYCALRDNALTAPEYRAFPHTPLPVEELQNGADVAPP
                     PLVKGYLRLGAKICGAPAWDPDFNTADFLTLLRLSDINARYARHFLGEASPR"
     gene            complement(3421..3879)
                     /gene="sixA"
                     /locus_tag="VHV99_19315"
     CDS             complement(3421..3879)
                     /gene="sixA"
                     /locus_tag="VHV99_19315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HEX3637595.1"
                     /translation="MDLILWRHAEAEDFAASDLARALTTRGRKQAQNVAKWLRTRLPD
                     DALVLASPAVRTIQTAETLSDQYRVVRELAPNASADDVLKAAGWPKGIAPTVVIVGHQ
                     PTLGHVAAHLLGNSNASWPLKKAGLWWIESRERDGEDQAVLRAAMSPDLI"
     gene            complement(4054..4130)
                     /locus_tag="VHV99_19320"
     tRNA            complement(4054..4130)
                     /locus_tag="VHV99_19320"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4094..4096),aa:Pro,seq:tgg)
BASE COUNT          755 a         1266 c         1363 g          780 t
ORIGIN      
        1 gccggatcga ggccgatcgg cgtgagcacc ggcagcagtt cgtcgaagaa gtagttgcgc
       61 gcccattcgg tctgcgcttc gttccacgct tccgtgccgt gaaaatagat gccttctgct
      121 tcgagcgcgg tgagcaccgt gtcgtgcagc atggtgtact gccgatgcac gagccgctgc
      181 gcgcgctcga ccacgaggtc gtacacgtgc tgcagcgaca tgccgtccgg cgacagcgca
      241 ccggggttgt cgcgcatctg ttcctgcaat ccggccatgc ggacctcgaa gaattcgtcg
      301 aggttgctac tggtgatgca gatgaagcgc agacgttcga gcaaggggac ggaggggtcg
      361 gcagcttgtg ccaacacacg ctcgttgaaa cccagaatgc ccagctcgcg attcaataag
      421 gggtagcgaa tggacatcgg cagcgagaat ggaatgtcgg gaggggagac ggaaagacgc
      481 tcggaaattc tcacagtatg atgacgttgt aatgacattc ttgaagttac aaattctacg
      541 ctgtattcgc ggggtgtcgt caatatgacg cagtgcatat gggaccatag ggcaacccca
      601 ttgctgcgag gcgacatcgt gagctccggt acgcttagaa tggcgtcttt gatcagcgcg
      661 gccggccaca aggcgcccaa ggcatccagt ggacacccgg caccttccgc gtccaccgcc
      721 gccgcgctcg cgcgcatgga gtccgcttac tcgatggtta ctaccccgca actccttgct
      781 gccgtcgacc tcggctcgaa cagcttccgg ctgatcgtag gccgggtcga ggaaaccgac
      841 gcgggcagcc agatctatca ggtcgacgca ttgcgcgagc cggtgcgcct tgccgccggt
      901 ctgtcgcgcg acaagatgct cgatcgcgcc tcgcaggtgc gcggctggga tgcgctcaag
      961 cgcttcggcg agcggttgcg cgactttcat cccgatcacg ttcgggcggt tgccacgaat
     1021 acactgcgca tcgccaagaa cgccaacgag tttctcggcg aggcgcaagc cgcgcttggc
     1081 tttccgatcg aagtgatcgc cgggcgcgaa gaagcgcgcc tgatttacgc cggcgctgca
     1141 cactccgtgc ccgctagcgc gggcaagcgc ctcgtggtgg atatcggcgg cggctcgacg
     1201 gaattcatca ttggttcgca ctacacgccg atcaagatgg agagcctgta tatcggctgc
     1261 gtgagccata gccgcgcctt cttcccggcg ggtaatgtcg acgaatacac catgcggcag
     1321 gccgaactcg ccgcaggccg cgaaatccag atcatttccg ccgagtacaa gaaaaccggc
     1381 tgggagcagg cgatcggctc gtccggcacc gcacgcgcgc tcgccgaact ggtcgaggca
     1441 aacggcttca acgatccggg tatcacacac ggcatctcgc gcggcgggct ggagcgcctt
     1501 aagcgagcgc tgatcaaggc cgagaacgtc aatcgcctga agctgatcgc gttgaagagc
     1561 gatcgcattc ccgtgctggc cggcggcctg tcgatcatga tcgcggtgtt cgacgaactc
     1621 ggcgtcgatt acgtcgatac gaccgacggc gcgctgcgcc tcggcgtgct gtacgacttg
     1681 ctgggccggt cgcagcacga ggacatgcgc accgtgacgg cggaaggctt catgcggcgt
     1741 tacggcgtgg accgcgcgca ggccgggcgt atcggcgaac tcgcggtgcg cttttacgac
     1801 cagtttgtcg agccggacga ggagcgccgc gtcgagaacc gcatgttcct cggctgggcc
     1861 gcggccttgc atgaaatcgg actgtcgatc tcgcacagcg catatcacaa gcattcggcc
     1921 tatatcgcga gcaatgccga catgccgggc ttttcgcgta ccgaccaggc gcggcttgcc
     1981 gcgctggtgc tcggccacgc ggggaaactc ggcaagctgt cgcaaacgcg cgaggtggag
     2041 tggccgctgt tgttctgtct gcggctcgcg gcgctgctat gtcgccgccg tgcggatgtc
     2101 ggcttgcccg gcatcgcggt tacacaggtc aatggggggt atgaagtgcg tttgccgaac
     2161 gagtgggtgg cgaacaatcc gctgaccgat tacagcctga tccaggaggc ggcggagtgg
     2221 gagaaggttg ggatctccta tcgcgtggtt tataccgacg attgagactc atgcgttgtt
     2281 ccgtgcctcg cacggacaca accgaatgcc gccgcgcgat cggcgcggca aataaaaaag
     2341 cccggcggag atgaaccgcc gggctttttt tatccgcatc agtcggtgac gcaagtcgac
     2401 tgaatggagc gcgtctttgc gcgccgttta gcgcggcgac gcttcgccga ggaagtgccg
     2461 cgcgtaacgc gcgttgatgt ccgacaggcg cagcagcgtc agaaagtccg ccgtgttgaa
     2521 atcggggtcc caagccggcg cgccgcaaat cttcgcgcca agacgcagat agccctttac
     2581 cagcggtggc ggcgcgacgt cggcaccgtt ctgcaattct tcgacgggca gcggcgtgtg
     2641 cggaaacgcg cgatattccg gcgccgtcaa cgcgttgtcg cgcagcgcgc agtacagatt
     2701 tgccgcgtag tggccgccgt cgaccatcgc gacgctcgcg cagccgagca tcgtctcgta
     2761 gccgttgtgc ttcatgtacg cggcgagccc ggcccacagc gacatgatca ccgcgccgct
     2821 gcggtagtcc gggtgcacgc acgaacggcc cacctcgacc atcttcgcgc gcaggtgtgt
     2881 gagacgcgac acgtcgaatt cgctttccgc atacagacgt ccgatacggg ctgcctgatg
     2941 cggcggcagc gcgcggtacg tgccgaccac cttcagcgtg tcgagatcgc gcaccagcag
     3001 gtgatcgcag tatgagtcga acgaatcgac gtcgaggccg gccggacccg tcagacgtgc
     3061 gcccatttca tcggcgaata cacggtagcg cagacgctgg gcttcgcgca gttcctcatc
     3121 ggtacgggcc cacgtgactt gcagccgatg ctgggcggtg accgtctctt cggcgcgcgg
     3181 caggcgccgc gtctcgaaga tcgaagccag gggcagggtg ggcgtcggca gttctcgcat
     3241 gtggcgtcct ggtcgaaaaa agggagattt cttttttcgc caatgtagta acgcctggtg
     3301 acgctcgcat ggcaaagcca tgacgattgg atgactgccc gtaagctacg tgcgtgcggc
     3361 ttgtaatgtc gtgagccacg cgcgccctgt gttgtttctg tgcgaccggc tcagggcgga
     3421 tcagatcaga tcggggctca tcgccgcgcg cagcacggcc tggtcctcgc cgtcgcgttc
     3481 acggctttcg atccaccata ggccggcttt cttcagcggc caactcgcat tgctgttgcc
     3541 gagcaggtga gccgcaacgt ggccgagcgt cggttgatgg ccgacgatca cgactgtcgg
     3601 cgcgatgcct tttggccagc cggcggcctt cagcacatcg tccgcactgg cgttgggcgc
     3661 aagttcgcgc accacgcgat actggtcgct caaggtttca gcggtctgga tggtgcgcac
     3721 ggcgggactc gcgagcacca acgcatcgtc gggcaggcgg gtgcgcagcc acttggcgac
     3781 gttttgcgcc tgtttgcggc cgcgcgtggt gagcgcgcgg gcgagatcgc tggcggcgaa
     3841 atcttccgct tcggcgtgac gccagaggat cagatccatg cgtgcctcct tgactctggt
     3901 ttcggcgtag acaactgcgc ccgggtttcg atcactgtcg catgggcgct cgtagggacg
     3961 caaggctggg caccagttgg catgaactca tgacgcccaa aaagcaaaaa cccgcgaagc
     4021 cgaaggcgac gcgggtttca tattgctttt tcgtggtcgg agcgaaagga ttcgaacctt
     4081 cgaccctctg atcccaaatc agatgcgcta ccaggctgcg ctacgctccg acgaatccga
     4141 gattctacgt ggcattgggt gcca
//
LOCUS       DATASH010000731         3356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6249398,
            whole genome shotgun sequence.
ACCESSION   DATASH010000731 DATASH010000000
VERSION     DATASH010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6249398"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VHV99_19325"
     CDS             complement(<1..324)
                     /locus_tag="VHV99_19325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX3637596.1"
                     /translation="MLRNCRHLRVLTWHVHGNYLYYLTHAPHDFYLVTKPGYPPGYAG
                     RVGVLPWGDNVHEIGIDEVPDRQFDVILYQHRTHWEHDREHVLSVEQQRLPRVYIEHD
                     PPQDNP"
     gene            complement(314..1453)
                     /locus_tag="VHV99_19330"
     CDS             complement(314..1453)
                     /locus_tag="VHV99_19330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX3637597.1"
                     /translation="MNAAPFLATLAPRRIVIFRALQLGDMLCAVPALRALRQAAPQAH
                     IALIGLPWAQAFVERYADLVDELIVFPGAVGFPEQHESNAGLPAFFSQMRARRFDLAI
                     QLHGSGGIANDLLFALGARANAGFVQPDEAARPDVFIGWPDGSPESERYLALMNALGA
                     PVVDRQLWFPLGTRDGDEYAALVAEHGIEAQRLVLIHPGAQLPSRRWPAGRFADVANR
                     LAADGWQIAITGTAAEAPLTGAVLGAMTAPALPLAGATSLGGLAALVAHARLVVCNDT
                     GVSHIADATATASVVIASGSDTRRWAPLDHARHRVLADYPACRPCMFRDCPYGHPCAL
                     NVSVEQVVQSARAQLAHDSGAHPGAPNALHASADPETFEERHHVA"
     gene            complement(1450..2529)
                     /locus_tag="VHV99_19335"
     CDS             complement(1450..2529)
                     /locus_tag="VHV99_19335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3637598.1"
                     /translation="MSSPVSGPVNLGGDFAGPRCTAEPVAASCPGALLAHRDVSVVVP
                     TYRRPAMLAACLDALCQQDFPADRYEIVVCDDGPDEATRACAERIGEAQAARGLTVRY
                     VPVTRTQGPAGARNAGWRLARSPLIAFTDDDTLPDPHWLSAGVAALAPGAAAAAGTIL
                     VPLPPLSTDYEADASGLERAEFATANAFVARRFLSATGGFDERFTCAWREDSDLQFAL
                     MRAGGEIVRASDAVVVHPVRPARWGVSIAQQRKSQFDALLYRKHPELFVTRICARPPL
                     LYYTILCAALTALIGGLARNPAVALGGLVAWSVLTASFCAMRLRGRDRSWRHIAEMAW
                     TSIPVPFLSIFWRIYGAVRFKVFFL"
     gene            complement(2526..>3356)
                     /locus_tag="VHV99_19340"
     CDS             complement(2526..>3356)
                     /locus_tag="VHV99_19340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX3637599.1"
                     /translation="TGLRTLSMAGGVALNCVMNSKLRDAGPFEDVWVQPAAGDAGTAL
                     GAALWIDYRERCARGDRQRRWIMDHAYLGPEYGDAEIEQFLRRSKVPYRKLADIAGET
                     AGLLAHGKVIGWYQGRTEFGPRALGARSILASPVDPHMQATLNEIKDREDFRPVAPVV
                     MEEHASDWFLGGKAAPFMLFVFDVRADKADRIPAVRHVDGTARVQTVNRTQHPLYYDL
                     LAAFRQRTGVPILVNTSFNTRGEPMVNSPRDAVESFWTSPLDALVIGPFLVEKPGVHA
                     "
BASE COUNT          598 a         1122 c         1134 g          502 t
ORIGIN      
        1 tggattgtcc tgaggcggat cgtgctcgat atagactcgc ggcaggcgct gttgctccac
       61 gctcaacacg tgctcgcggt catgttccca gtgagtcctg tgctgataca ggatcacgtc
      121 gaactgccga tcgggcacct cgtcgatacc gatctcgtgc acgttgtcgc cccacggcag
      181 cacgccaacc ctgcccgcat agccgggcgg atagccgggc ttcgtgacca gatagaagtc
      241 gtgcggcgcg tgggtcaggt agtacagata gttgccgtgc acgtgccagg ttagcacgcg
      301 cagatggcgg caattacgca acatgatgcc gctcctcgaa agtttccggg tccgcagatg
      361 catgcagcgc gttcggcgcg ccgggatgcg ccccgctgtc gtgagcgagt tgcgcgcgcg
      421 cggactggac cacctgctcc acgcttacat tcagcgcgca tggatgacca taagggcagt
      481 cgcggaacat acatggccgg cacgcgggat agtcggcaag tacgcgatgc cgtgcgtgat
      541 cgagcggcgc ccagcgtcgg gtgtcgctgc cggacgcgat cactaccgat gcggtggccg
      601 tggcgtcggc aatgtgcgag acgcccgtgt cgttgcagac caccagtcgg gcatgcgcga
      661 ccagcgcggc cagtccgccg agcgacgtgg cgccggccag gggcagcgcg ggcgcggtca
      721 ttgcgccgag caccgcgccg gtcagcggcg cttcggcggc ggtgccggtg atggcgatct
      781 gccagccgtc ggcggccagg cggttggcca cgtcggcaaa gcgtccggcg ggccagcgac
      841 gcgacggcaa ttgcgcgccc gggtgaatca gcacgagccg ctgcgcttcg atgccatgct
      901 cagccaccag cgcggcgtac tcatcgccgt cgcgcgtgcc cagcgggaac cacaactggc
      961 ggtcgaccac aggcgcgcca agcgcattca tcagcgcgag atagcgttcg gactccggcg
     1021 acccgtcggg ccagccgatg aaaacgtcag gtcgcgccgc ctcgtcgggc tgcacgaagc
     1081 ccgcattggc ccgcgcgccg agtgcgaaca gcagatcgtt cgcgatgccg ccgctgccgt
     1141 gcagctggat cgccagatcg aaacgccgtg cacgcatctg cgaaaaaaat gcgggcagcc
     1201 cggcgttcga ttcgtgctgt tcgggaaagc cgaccgcacc gggaaacaca atcagctcgt
     1261 cgacgaggtc cgcgtagcgc tcgacgaacg cttgcgccca tggcaagccg atcaacgcga
     1321 tgtgcgcctg cggcgccgcc tgtcgcaacg ctcgcaacgc gggcacggcg cacagcatgt
     1381 cgcctagttg tagggcgcga aagatgacga tgcggcgcgg cgcgagcgtc gccagaaacg
     1441 gagctgcatt catagaaaga acaccttgaa acggacggcg ccatagatcc gccagaagat
     1501 ggaaaggaac ggaactggaa tcgatgtcca cgccatttcg gcaatatgcc gccagcttcg
     1561 atcgcggccg cgcaggcgca tcgcgcagaa actcgcggtc agcacagacc aggcgacgag
     1621 accgccaagc gctaccgccg gattgcgggc aagcccgccg attagtgccg tcaatgccgc
     1681 gcagagaatg gtgtaataga gcagcggagg ccgcgcacaa atccgtgtta cgaatagttc
     1741 gggatgcttg cgataaagca gcgcatcaaa ctggctcttg cgctgctggg cgatgctcac
     1801 cccccagcgc gcgggccgca ctggatgcac gaccaccgca tcgcttgcgc gcactatctc
     1861 gccccctgcc cgcatcagcg cgaattgcag atcggagtcc tcgcgccacg cgcaggtgaa
     1921 gcgctcgtcg aacccgcccg tcgcactgag aaagcgacgg gcgacgaagg cattcgcggt
     1981 cgcgaactcg gcgcgttcca ggccactcgc gtccgcttcg tagtcggtcg acagcggtgg
     2041 cagcggcaca aggatcgttc cagcggcggc cgcagcgccc ggcgcgagcg ccgcgacgcc
     2101 ggcgctcagc cagtgcggat cgggcagcgt gtcgtcgtcg gtgaacgcga tcagcggcga
     2161 acgcgcgagc cgccagccgg cgttgcgtgc gccggcggga ccctgcgtgc gcgtgacggg
     2221 cacatagcgc accgtaagtc cgcgcgcggc ctgggcctct ccaatacgct cggcgcacgc
     2281 gcgcgtggct tcgtccggac cgtcgtcaca gaccacgatt tcgtagcggt ccgctggaaa
     2341 atcctgctgg cacagcgcgt caagacacgc ggcaagcatc gccggccggc gataggtcgg
     2401 cacgaccacg ctgacgtccc ggtgcgccag caacgcaccc gggcagctcg cggcaactgg
     2461 ctcggcggtg cagcgcggac cggcgaagtc gccgcccaga ttgaccggac cggacactgg
     2521 tgaactcatg cgtggactcc cggtttctcg acgaggaacg gcccgatcac gagggcgtcg
     2581 agcggcgagg tccagaacga ttccaccgcg tcacgcggcg agttcaccat cggctcgcca
     2641 cgggtgttga aggacgtgtt caccagaatc ggcacgccgg tgcgttgcct gaacgcggcc
     2701 agcaggtcgt agtaaagtgg atgctgcgtg cggttgacgg tttgcacgcg cgccgtaccg
     2761 tccacgtggc gcacggcggg aatgcgatcg gccttgtcgg cgcgcacgtc gaaaacgaac
     2821 agcatgaacg gcgcggcctt cccgccgagg aaccagtcgc ttgcatgctc ttccatcacg
     2881 accggcgcaa ccgggcgaaa gtcctcacga tccttgatct cgttgagcgt cgcctgcatg
     2941 tgcggatcga ccggcgacgc gaggatcgaa cgggcaccga gcgcacgcgg cccgaactcc
     3001 gtgcgaccct gataccagcc gatcactttg ccgtgggcga gcagaccggc ggtttcgccg
     3061 gcgatgtcgg cgagcttgcg gtacggcact ttcgaccggc gcaggaactg ttcaatctcc
     3121 gcgtcgccgt attccgggcc gaggtacgcg tggtccatta tccagcggcg ctggcggtcg
     3181 ccccgtgcgc agcgctcgcg gtaatcgatc catagcgccg cgccgagcgc ggtgcccgca
     3241 tcgccggccg cgggttggac ccagacgtcc tcgaacggcc cggcatcgcg cagcttcgaa
     3301 ttcatcacgc agttcagcgc gacgccgccg gccatgctga gggtgcgcag ccccgt
//
LOCUS       DATASH010000732         3013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5660106,
            whole genome shotgun sequence.
ACCESSION   DATASH010000732 DATASH010000000
VERSION     DATASH010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5660106"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="VHV99_19345"
     CDS             complement(<1..607)
                     /locus_tag="VHV99_19345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009911299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEX3637600.1"
                     /translation="MTRDPRLTLNARQQELLEWVQRDGFVTVDDLAAHFDVTPQTIRR
                     DVNWLADMNLLRRYHGGASLPTSSENVSYTARQRMFHDEKRRIATLVATHIPDQASLF
                     INLGTTTEEVARALNRHRGLRVITNNLNVASMMSGYPDCEVLVTGGIVRPWDKGIVGE
                     LAIDFIRQFKVDFAIIGTSSIETDGTLRDFDTREVRVAEAII"
     gene            947..2584
                     /gene="ggt"
                     /locus_tag="VHV99_19350"
     CDS             947..2584
                     /gene="ggt"
                     /locus_tag="VHV99_19350"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX3637601.1"
                     /translation="MTGFNWQNPYPTPRLPVFARNIVSTSHPLAAQAGLRMLWKGGNA
                     VDAAIAAAAAITVVEPVSCGLGGDAFALVWDGKKLHGLNASGVSPAAWNVDYFKRKYG
                     EENGLARQPKRGWDAVTVPGVIAGWEALHQKFGSLPFADLMEPAIEIAERGHAVASIV
                     AYKWAAAVPELKDLPGFAQTFMPRGRAPEVSELVRFPGHAKTLRKLAEHGPRAYYEGE
                     IAEQIAAFAREGGGALTLDDLRNYRADWVEPIGKDYRGYTVHEIPPNGQGIAALIALG
                     ILEKFDVKELAVDNVESQHLQIEAMKLAFADVYRYVADPRSMEVTPEQMLDDAYLTSR
                     AKLIDHKRATQFDFGMPKAGGTIYMSVADERGMMVSFIQSNYMGFGSGIVVPDSGISM
                     QNRGCGFSMDPKSPNVVEGGKRPFHTIIPSFLTQQVDGKQEAVMSFGVMGGDMQPQGH
                     LQSIVRMLDYGQQPQAACDAPRWKVNRDFTIDIESTLDPKTAAALQKLGHTIKSVDDP
                     YMDFGSGQYIWKLDRNDPDRGYVAASDSRRDGLAAGF"
     gene            2606..3013
                     /locus_tag="VHV99_19355"
                     /pseudo
     CDS             2606..3013
                     /locus_tag="VHV99_19355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090472.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT          530 a          916 c          978 g          589 t
ORIGIN      
        1 cgatgatcgc ctcggcaacg cgcacttcgc gggtgtcgaa atcgcgcagc gtgccgtccg
       61 tttcgatgct cgacgtgccg atgatcgcga agtccacctt gaactggcgg atgaaatcga
      121 tcgccagttc gccgacgatc cccttgtccc acggccgcac gatgccgccc gtcaccagca
      181 cttcgcaatc cggatagccg ctcatcatgc tggcgacgtt cagattgttg gtgatcacgc
      241 gcaggccgcg atgccggttg agcgcccgtg cgacttcctc ggtggtcgta ccgaggttga
      301 tgaagaggga cgcctggtcg ggaatgtgag tggccactaa cgtcgcaatg cgccgcttct
      361 cgtcatggaa catgcgctga cgcgccgtgt acgagacgtt ctcggagctg gtcggcaggc
      421 tggcgccgcc gtggtagcgg cgcagcaggt tcatgtcggc gagccagttg acgtcgcggc
      481 ggatcgtctg cggcgtcacg tcgaagtggg ccgcaaggtc gtccacggtc acgaagccgt
      541 cgcgttgcac ccactccagc agttcctgtt gccgggcatt gagagtcagg cgggggtctc
      601 gggtcatgtg tctcgggtcg tggggttttt atcggcgctc tccgctgccg gcgcggtgag
      661 ctgagccgga aaaacgggta aggcgtggac gagggtgacc gtattgtaag accatcgcac
      721 gtcttgtctg ctcgtgtgtc atggtttgga tccaggacgg ctgtagcgcg atccattcaa
      781 gtacgttttg catttattca ttcgataaat gaatttgttt tttgtgccgg atcgcgctgc
      841 aatccaaggc tccgcgtttt accccgtgtt accccgtttt cacccctttc cccatggtct
      901 ccggcgttga ggatgcgtcg ggccgcgctt tcgagagtgt tccacaatga ctggtttcaa
      961 ctggcaaaac ccctatccga cgccgcgtct gcccgtattc gcgcgcaaca tcgtttcgac
     1021 ttcacatcca ctcgccgcgc aagccggcct gcggatgttg tggaaaggcg gtaatgccgt
     1081 cgacgcggcg atcgccgccg ccgccgccat cacggtcgtc gaaccggtct cttgcggtct
     1141 cggcggtgac gccttcgcgc tggtgtggga cggcaagaag ctgcatggcc tgaacgcgtc
     1201 gggcgtgtcg ccggcggcgt ggaacgtcga ctacttcaag cgcaagtacg gcgaggaaaa
     1261 cggtctcgcc aggcaaccca agcgcggctg ggacgccgtg accgtaccgg gcgtgatagc
     1321 cggctgggaa gcgctgcatc agaaattcgg ctcgctgccg ttcgccgacc tgatggagcc
     1381 ggcgatcgaa atcgccgaac gtggtcacgc ggtggcgagc atcgtcgcct acaaatgggc
     1441 tgcggccgtg ccggaactga aagacctgcc gggcttcgcg caaaccttca tgccgcgcgg
     1501 ccgcgcgccg gaagtcagcg agctcgtgcg ctttcccggt cacgcgaaga cgctgcgcaa
     1561 gctcgctgaa cacggcccgc gtgcgtacta cgaaggcgag atcgccgaac agatcgccgc
     1621 gtttgcgcgc gaaggcggcg gcgcgttgac gctcgacgac ctacgcaact accgcgcgga
     1681 ttgggtcgag ccgatcggca aggattatcg cggttacacc gtgcacgaaa ttccgccgaa
     1741 cggtcagggc attgccgcgt tgatcgcgct cggcatcctc gaaaagttcg acgtgaagga
     1801 actcgctgtc gacaacgtcg agtcgcagca tttgcagatc gaagccatga agctggcgtt
     1861 cgccgacgtc taccgctacg tcgccgatcc gcgttcgatg gaagtcacgc ccgagcagat
     1921 gctcgacgat gcgtacctca cgtcgcgcgc gaagctgatc gaccacaagc gcgccacgca
     1981 attcgacttc ggcatgccga aggcgggcgg cacgatctat atgtcggtgg cggacgagcg
     2041 cggcatgatg gtcagcttca tccagtcgaa ctacatgggc ttcggctcgg gcatcgtggt
     2101 gccggacagc ggcatctcga tgcagaaccg cggctgcggc ttctcgatgg acccgaagtc
     2161 gccgaacgtg gtggaaggcg gcaagcggcc gttccacacc atcatcccgt cgttcctcac
     2221 gcagcaggtg gacggcaagc aggaagcggt aatgagcttc ggcgtgatgg gcggcgacat
     2281 gcagccgcaa ggccatctgc aatcgatcgt gcggatgctc gactacggtc agcaaccgca
     2341 ggccgcctgc gacgcgccgc gctggaaggt caatcgcgac ttcacgatcg atatcgagtc
     2401 gacgctcgat ccgaagaccg ctgcggcgct gcagaagctg ggccacacga tcaagtcggt
     2461 ggacgatccg tacatggact tcggctccgg ccagtacatc tggaagctcg atcgcaacga
     2521 tccggaccgc ggctatgtgg cggcgagcga cagccgccgc gacgggttgg ccgctgggtt
     2581 ttaaacgacg cccgaggaca agggcgcgtc tttctccaac caatgggtga atctgttact
     2641 ggccgtgggc atcgtcgcgc aatcgacggt cggcgtgtat gtgctgcaac tctacatgcc
     2701 gatgtacgcg gtcaaacagt tgcacatgcc ggcggcggcg tcgttcggcg tggtggtgct
     2761 caacggcggc ctgcagtttc tgctctcgcc ggtgatgggc gcgttgtccg accgcatcgg
     2821 ccggattcgc atcatgctaa ccacgtcgat tctgatgggc gcgctgatct gtccgatgtt
     2881 cgcatggttg cagtcgcatc cgacgatcgg ctggctgttg gtgttgcaag gcacagccgg
     2941 tattttcaag gccgcttact ctggaccgat gtccgcgctg atgtccgaga tcttcccgac
     3001 gcagatgcgc tcg
//
LOCUS       DATASH010000733         4433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4865855,
            whole genome shotgun sequence.
ACCESSION   DATASH010000733 DATASH010000000
VERSION     DATASH010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4865855"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VHV99_19360"
     CDS             complement(<1..302)
                     /locus_tag="VHV99_19360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX3637602.1"
                     /translation="MALQSSTGSRFATGRTRDIVCLGRLAVDLYAQQVGARLEDVSSF
                     AKYLGGSSANIAFGCARLGLKSAMLSRVGNDHMGRFLTETLEREGCDTSHVRIDRDR"
     gene            complement(355..1173)
                     /locus_tag="VHV99_19365"
     CDS             complement(355..1173)
                     /locus_tag="VHV99_19365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019256136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3637603.1"
                     /translation="MSDPIQSAPPAPADTILALENVSKFFGKVIALNGVTLRLRRGEV
                     HCLLGDNGAGKSTLIKTLAGVHTPSSGEYLVDGKPVRFGSPKEALDLGIATVYQDLAL
                     VPLLSVARNFFMGREPQKKRFGFINVMDLDLAAETSRARLSEMGINVRDAHQPIGTMS
                     GGEKQCLAIARAIHFGARVLILDEPTAALGVKQSFNVLKLIHTARSKGISVIFITHNV
                     HHAYPIGDSFTLLNRGKSIGTFTKDTITKNEVLDMMAGGAEMQQMMNELEDVPA"
     gene            complement(1204..2385)
                     /locus_tag="VHV99_19370"
     CDS             complement(1204..2385)
                     /locus_tag="VHV99_19370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3637604.1"
                     /translation="MGVAGKPILSGHRKPSDGAATSSSSTATAAASADERIRRESRFG
                     HILNRPEFAAISGAVLVFVVFGIAAGSSGMFNLDGIMNWSQVSAYLGILAVGACLLMI
                     AGEFDLSIGSMIGFAGMMVAIPTVYFHWPIWLAIVFAFAGCMLLGALNGYIVMRTRLP
                     SFIVTLAFLFILRGLTLALSIMFADRTIVSGVGDIAAKDWLANTLFHGVALRDLFVWL
                     GHIGILKMLDNGQPLVPGIPKVILWWVVLAAIGAFVLAKTRFGNWIFAAGGDAVAAKN
                     VGVPVRRVKISLFVLTAFCACLFAVLQVCDIGSAAADRGLEKEFEAIIAAVIGGTLLT
                     GGYGSVVGACFGALIFGVVQIGIGYTSVPSDWFRVFLGMMLLIAVLFNHYVRRRVSQA
                     Q"
     gene            complement(2564..3616)
                     /locus_tag="VHV99_19375"
     CDS             complement(2564..3616)
                     /locus_tag="VHV99_19375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX3637605.1"
                     /translation="MRLCNRRGALRALAAAAALCGAAALSSQAAHAAPDAHFVLISHA
                     PDSDSWWNTIKNAIKQADEDFNVQTDYRNPPNGDIADMARLIEQAAARNVDGVIVTIA
                     DFDVLKGAISKVTAKHIPLVTINSGTEEQSAQLGAIMHVGQPEYTAGKAAGEKAKAAG
                     IKSFLCVNHYATNPASFERCRGFAEALGVDFKSSTLDAGQDPTTVQSKVSAYLRNHPD
                     TQAVLALGPLSADPTIKALKQMGVAGKLWFATFDFDSDIAKAIQDGTIQFAIDQQPYL
                     QGYIPVAVLAIVKKDHTTDPVKIRQALEANPKFKARLATYGLTPSYGPRNIGSGPGFI
                     TKENVDKVLKYAGQYR"
     gene            3837..>4433
                     /locus_tag="VHV99_19380"
     CDS             3837..>4433
                     /locus_tag="VHV99_19380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012400988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEX3637606.1"
                     /translation="MQEDTQDPQETAETLTVDELMQRITEAYDALPRQLKSVATYIEQ
                     HRSNVMMDRTGDIASACGVHPSAVVRFAQRFGFSGFSDLQAIFKQAYTGQNGSAQSYQ
                     QRIRKLIDEKPGKLSGGSVAREFIAASRSGLEELEAGLDDIQFEAAVKMLEHAENIYV
                     IGVRRSFPVASYIVYALQHTAKRVHLVSGFGGMYREQIR"
BASE COUNT          910 a         1300 c         1354 g          869 t
ORIGIN      
        1 cggtcgcgat cgatccgcac atgactcgta tcgcagcctt cacgttccag agtctcggta
       61 agaaaccggc ccatgtggtc gttgccgacg cgcgagagca tcgcggattt gaggccgagc
      121 ctcgcgcacc cgaacgcgat attcgccgac gacccgccaa gatatttcgc aaagctcgat
      181 acgtcttcga gccgggcgcc gacttgttgc gcgtagagat cgaccgcgag ccgcccgagg
      241 cacacgatgt cgcgggttcg cccggttgca aagcgcgatc cggttgatga ttgcaatgcc
      301 atgttcgatc ctgttctgta gatgacgaag agtgcagtag ccgcgcgtgc cggctcaagc
      361 tggcacgtct tccagttcgt tcatcatttg ctgcatttcg gcgccgcccg ccatcatgtc
      421 gagtacttcg ttcttggtga tcgtgtcttt agtgaacgta ccaatcgact tgccgcgatt
      481 gagcagcgtg aacgaatcgc cgatgggata cgcgtgatgc acgttgtgcg tgatgaagat
      541 caccgagatg cctttcgagc gcgccgtgtg aatcagcttc agcacattga aactctgctt
      601 cacgccgagt gcggcagtgg gttcatcgag gatcagaaca cgtgcgccga agtggatcgc
      661 ccgtgcaatg gcgaggcatt gcttctcgcc gccggacatc gtgccaatag gctgatgcgc
      721 atcccgcacg ttgatgccca tttcgctgag ccgcgcgcgc gatgtttctg cggcgagatc
      781 caggtccatc acgttgataa agccgaagcg ttttttctgc ggctcgcgtc ccatgaagaa
      841 gttgcgcgcc accgacagca gcggcaccag cgcgagatcc tgataaaccg ttgcaatgcc
      901 cagatcgagc gcttctttcg gcgagccgaa ccggaccggt ttgccatcga cgagatattc
      961 cccggaggag ggcgtatgca cgccggcgag cgtcttgatc agtgtggatt taccggcgcc
     1021 gttgtcgcca agcaggcagt gcacttcgcc gcgtctcagg cgcagcgtga cgccgttcag
     1081 cgcgatcacc ttgccgaaaa acttgctgac gttttccagt gcgaggatgg tgtcggcggg
     1141 cgcaggcggt gcgctctgga tgggatcgga catgatttgt tctcctttcc ttattgcgct
     1201 tgtttattgg gcctgtgaaa cgcggcggcg cacgtagtga ttgaacagca cggcgatcag
     1261 cagcatcatg ccgaggaaca cgcggaacca gtcagacggc acgctggtgt agccaatgcc
     1321 gatctggacc acgccgaaga tgagcgcgcc gaaacatgcg ccgactaccg agccgtagcc
     1381 accggtcagc aaggtcccgc caatcaccgc ggcaatgatc gcctcgaatt ccttttcaag
     1441 cccccggtct gccgcagccg aaccgatgtc gcacacctga agcacggcaa acaggcacgc
     1501 gcagaatgcg gtgagcacaa acagcgagat cttcacgcgc cgcaccggca cgccgacatt
     1561 cttggccgct accgcgtcgc cgccggccgc aaagatccag ttgccgaaac gtgtcttggc
     1621 caggacgaac gcgccaatgg cggcaagcac gacccaccag aggatcactt tcggaatgcc
     1681 cggtacgagc ggctggccgt tgtcgagcat cttcaggatt cctatgtgcc ccagccacac
     1741 gaacaaatcc cgcagggcga cgccatggaa aagagtattg gcaagccagt ctttcgcggc
     1801 aatgtcacct acgccggaaa cgatggtccg gtcggcgaac atgatcgaga gggcaagcgt
     1861 caggccgcgc aggatgaaca ggaaagccag cgtgacaatg aacgacggca accgtgtgcg
     1921 catgacgata tagccgttca gcgcgccgag cagcatgcag cccgcaaagg caaaaacgat
     1981 cgcgagccag atcggccagt ggaaatagac ggtgggaatg gcgaccatca tcccggcaaa
     2041 accgatcatg gagccgatcg agaggtcgaa ctcgccggca atcatcaaca ggcaggcgcc
     2101 gacggccagg atgccgaggt aagcggacac ctgcgaccag ttcatgatgc cgtcgagatt
     2161 gaacatgccc gagctgccgg cggcgatccc gaacacgacg aagaccagca cggcgccgga
     2221 gatcgcggcg aattccggac gattcagaat gtgcccgaaa cgcgattcgc ggcggatgcg
     2281 ttcgtcggcg gaggctgctg cggtggcggt ggatgaactc gatgtcgcgg cgccgtcaga
     2341 cggcttgcga tgtcccgaga ggataggttt tccggctacg cccatgatgt ctccttcgtc
     2401 tttcattcgc gatgcggcgt gggttcacag ggcgaacatg ctgatgcgcg agcgatacag
     2461 gtgaatggtg cggatgacgt gatgaatgcc tgaaggtgtg ttcaaagatg gcggcgtggc
     2521 ttgcaacacg cttaaggtca aactaatcgc gccgccatcg ggttcatctg tactgacctg
     2581 cgtacttgag gaccttgtcg acgttttcct tggtgataaa gcccggaccg gagccgatat
     2641 tgcgcggccc gtaggacggt gtcagcccat aggttgccag ccgtgccttg aacttcggat
     2701 ttgcttccag ggcctggcga atcttgaccg ggtcggtcgt gtgatccttc ttcacgatag
     2761 ccagcaccgc gacggggata taaccctgca agtacggttg ctggtcgata gcaaactgga
     2821 tcgtgccatc ctggatcgcc tttgcgatgt cgctatcgaa gtcgaaagtc gcgaaccaga
     2881 gcttgcccgc cacgcccatc tgcttgagcg ccttgatggt cggatctgcc gagagcgggc
     2941 cgagtgcgag cacggcctgc gtatccgggt gattgcgcag atatgcgctc accttggatt
     3001 gaacggtcgt cggatcctgg cctgcgtcca gcgtggacga cttgaaatcg acgccaagcg
     3061 cttcggcgaa accccggcaa cgctcgaacg aagccggatt cgtcgcgtag tggttgacgc
     3121 acaggaacga cttgatgccg gccgctttcg ccttctcgcc cgccgcctta ccggccgtgt
     3181 attcaggctg gccgacgtgc atgatcgcgc cgagctgcgc gctttgctct tcggtgccgg
     3241 agttgatcgt gaccagcgga atgtgcttcg cggtgacctt ggagatcgcg cccttcaaca
     3301 cgtcgaagtc ggcgatcgtg acgatcacac cgtcgacatt acgagccgcc gcctgttcga
     3361 tcaggcgcgc catatcggca atatcgccgt tcggcgggtt gcggtaatcc gtctggacat
     3421 tgaagtcctc gtccgcctgt ttgatcgcgt tcttgatcgt gttccaccac gagtccgaat
     3481 ccggcgcatg gctgatcaaa acgaaatggg cgtcgggagc ggcatgcgct gcctggctgc
     3541 tcagtgctgc tgcgccgcac aacgcagcgg cagccgcgag cgcgcgcagg gcgcctcggc
     3601 gattgcaaag tctcattgtc tccacctatc acggttgttg ttctgaacga gcatccggca
     3661 tcttgcgtac ggcgcttgac cgttcgacgc cccttgctca ggaccaagat taggacaagt
     3721 ttcgtcgctc tgcaatagga agtttaacta tgcgaagttt ggaaaattaa ttccatttgt
     3781 tcgaccaggt atctataatc ggttgcatcg aatacagaac aaaccacgag cacggcatgc
     3841 aggaagacac gcaagacccc caggagacgg ctgagacgct cacagtcgat gaactgatgc
     3901 agcggatcac cgaggcctac gacgcgctgc cgcgccagct aaagagcgtg gcaacgtaca
     3961 ttgaacaaca tcgcagcaac gtgatgatgg atcgcaccgg cgacatcgcc agtgcgtgcg
     4021 gtgtgcatcc atcggctgtc gtgcgtttcg cccagcgctt cggtttctcc ggcttctctg
     4081 atctccaggc aatcttcaag caggcctaca cgggccagaa cggctccgcg cagagctatc
     4141 agcagcgcat ccgcaaactg atcgatgaaa agccgggcaa gttgtcgggc ggatcggtgg
     4201 cgcgcgaatt tatcgcggcg agccggagtg gactggagga actcgaagca ggtctcgacg
     4261 acatccagtt cgaagccgcc gtgaagatgc tggaacatgc cgagaacatc tacgtgatcg
     4321 gtgtgcggcg ctcgtttccg gtggcgagtt atatcgtcta cgcgctgcag cacacggcca
     4381 aacgcgtgca tctggtgtcg ggtttcggcg gtatgtatcg cgagcagatc cgc
//
LOCUS       DATASH010000734         8734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2493674,
            whole genome shotgun sequence.
ACCESSION   DATASH010000734 DATASH010000000
VERSION     DATASH010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 8734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8734
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2493674"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(246..572)
                     /gene="trxA"
                     /locus_tag="VHV99_19385"
     CDS             complement(246..572)
                     /gene="trxA"
                     /locus_tag="VHV99_19385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006026981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HEX3637607.1"
                     /translation="MSESIKHISDASFEQDVVKSDKPVLLDFWAEWCGPCKMIAPILD
                     EVAKDYADRLQIAKINVDEHQSTPVKFGVRGIPTLILFKNGAVAAQKVGALSKSQLTA
                     FLDGNL"
     gene            1019..1117
                     /gene="ffs"
                     /locus_tag="VHV99_19390"
     ncRNA           1019..1117
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VHV99_19390"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            1324..4164
                     /gene="dnaX"
                     /locus_tag="VHV99_19395"
     CDS             1324..4164
                     /gene="dnaX"
                     /locus_tag="VHV99_19395"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEX3637608.1"
                     /translation="MTYQVLARKWRPKDFASLVGQEHVVRALTHALDGGRLHHAYLFT
                     GTRGVGKTTLSRIFAKALNCETGVTSTPCGVCRACREIDEGRFVDYVEMDAASNRGVD
                     EMAALLERAVYAPVDARFKVYMIDEVHMLTNHAFNAMLKTLEEPPSHVKFILATTDPQ
                     KIPVTVLSRCLQFNLKQMPAGHIVSHLEHILGEEQVPYDAQALRLLARAADGSMRDAL
                     SLTDQAIAYSANQVNEEAVRGMLGALDQSYLIRLLDALADGDGAAVLAVADEMALRSL
                     SFSTALQDLASLLHRIAWAQFAPSSVLDEWPEAGDLRRFADALSAEQVQLFYQIVTIG
                     RSELGLAPDEYAGFTMTLLRMLAFEPAPTGGGGGAAAARSAGPAGARRVGSPAVAVQP
                     AVAVQQAVAVQPAVTVQPEVASSHTAEAIAAVPVRSANPLAEAKPATPRPQHLASAGS
                     AENADTPKPTMREAAIVSKSASASGSDEASAAPAPTAAEVVAAPALAPWQEETSGQPI
                     LSDPVPAPTDTSALRAASMPEATPPAAQATEATEATEASEASEASEATKVARALAPWD
                     DAPTEPAPNETPAAAASGQSAPVSSLTASAASGQPAAASSLVASAAPQQSAPASLLTA
                     AAVAALARAQEAAPATASQAPPAADIPPRRAGGASDALNVLRSAGLKVSSERGRTTAA
                     ASAKPIAAPATPKPAAPRVVVNVPTPGAARRAPQQDAAARAPSPQAQRDGVEQNGSGS
                     PGAPWDDMLPDEYMPLTAADEGYYGLPDDNYMPVFDSGPDDVRMNAAPASPPAPVVDK
                     RPLPAAVPLDPLGFKGDWPALAVDLPLKGISYQLAFNSELMALEGSTLKLNVPVPQYA
                     EASQVVKLKAALVEKLGQNLDVLVEVGPARRTAAAHDAAMRAQRQQEAEREIGADPFV
                     QSLIREFGASIVPGSIRPLTQDAVPNGTPSVH"
     gene            4228..4554
                     /locus_tag="VHV99_19400"
     CDS             4228..4554
                     /locus_tag="VHV99_19400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEX3637609.1"
                     /translation="MMKGQLAGLMKQAQQMQENMKKMQEQLALIEVEGQSGAGLVKVT
                     MTCKNDVRRVSIDPSLLADDKDMLEDLVAAAFNDAVRKAEATAQEKMGGMTSGLPLPP
                     GFKLPF"
     gene            4857..5453
                     /gene="recR"
                     /locus_tag="VHV99_19405"
     CDS             4857..5453
                     /gene="recR"
                     /locus_tag="VHV99_19405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEX3637610.1"
                     /translation="MKQPSALSALVEALRVLPGVGPKSAQRMAYHLMQHDRDGAEKLG
                     RSLLFATEHLQHCEKCNTFTEARICEVCLDEERDPALLCVVETPADQIMLEQTMTYRG
                     LYFVLMGRLSPLDGIGPKEIHFDRLVKRASDGIVKEVVLATNFTNEGEATAHYLGQTL
                     KARGLAVTRLARGVPVGGELEYVDAGTIARAMLDRRSM"
     gene            5589..6821
                     /locus_tag="VHV99_19410"
     CDS             5589..6821
                     /locus_tag="VHV99_19410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018432073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX3637611.1"
                     /translation="MSVTPDPGVTDTPKGPLAGVKVLELGTLIAGPFAARFLGEFGAD
                     VIKIEDPKGGDPLRKWRKLYPEAGGTSLWWAVQARNKKSVTINLKAEEGKEIVRRLAK
                     EADIVVENFRPGLLEKLGLGYDVLSAENPGLVMVRLSGYGQTGPYRDRPGFGAIAESM
                     GGLRHITGYPDLPPPRIGISIGDSIAALHGVIGALMALHHKQVNGGKGQVVDVALYEA
                     VFNMMESVVPEYGVYGMVRERTGASLPGIVPSNTYPCRDGSIVIGGNSDPIFKRLMIA
                     IEREDLANDPALAHNDGRVPRTQEIDGAIAAWLATRTIDEALAVLNAADVPVGRIYSV
                     ADMFTDPQFMARQMIQRFKWQDGKEITLPSVTPKLSETPGETRWLGPELGEHTDEVLQ
                     SLGYDADHIARLHAQQIV"
     gene            6977..8014
                     /locus_tag="VHV99_19415"
     CDS             6977..8014
                     /locus_tag="VHV99_19415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3637612.1"
                     /translation="MQRRTFLAGTAALAGATFAATPLAFAQGKPETTKVAIAVGGKNL
                     FYYLPLTIAERRNYFKDEGLEVEISDFAGGSQALKAAVGGSADVVSGAFEHTLLLQAQ
                     KQAFREFVLQGRAQQIVLAVSKKAMPNYKSIADLKGKKIGVTAPGSSTSIMASFVLAK
                     AGLTAKDVAFIGVGAGAGAIAALQSGQIDAIANLDPVMTKLERTGEIRVVSDTRTLAD
                     TRSVFGGDMPAGCLYASQSFITKNPNTTQALTNAMVRALKWLQTATGSELISTVPEGY
                     LLGDRAVYLDAWQHVKEAMSPDGLMPADGPATSLKTLQAFDPNVQGKPIDLSKTWTND
                     FVKKALTTVKA"
     gene            8119..>8734
                     /locus_tag="VHV99_19420"
     CDS             8119..>8734
                     /locus_tag="VHV99_19420"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3637613.1"
                     /translation="MTVAALALDNITCTFAARDNRAQRYTAVKDTTLRIAPGEFVSVV
                     GPTGCGKSTLLNVGAGLLEPSSGTVSVFGDPLKGINRRAGYMFQADALMPWRSAIDNV
                     MAGLAFHGVPPAEARSKADEWLKRVGLGGFGDRYPHQLSGGMRKRVAMAQTLILDPYI
                     ILMDEPFSALDIQTRQLMENELLDLWAAKRKAVLFITHDLDEAIA"
BASE COUNT         1662 a         2737 c         2788 g         1547 t
ORIGIN      
        1 taaatgcata cggagaaccc gcaggagaag gtgcgacgaa atgaaatctg ggaggagagt
       61 gagcggaacc gctcaaggac ttacacgtct tttcgacgtt ttgcggattc tagcatagca
      121 cacgccaatt tccgccagtg cgacggcatg gcatctttct tattgagcgt gttgccggct
      181 gacaacacgc tcaagacaac gcgcctgaag gtctcgaggg cccgcagcgc gccgatgcga
      241 ccgttttaca gattgccgtc gaggaatgcg gtgagttgcg acttcgacaa tgcgccgacc
      301 ttctgcgccg cgacagcgcc gttcttgaag aggatcagcg tggggatgcc gcgcacgccg
      361 aacttgaccg gcgtcgattg atgttcgtcg acgttgatct tggcgatttg caggcgatcg
      421 gcgtaatcct tggcgacttc gtcgaggatc ggcgcgatca tcttgcacgg accgcaccat
      481 tcagcccaga aatcgagcag cacgggttta tcggatttca cgacgtcctg ttcgaacgat
      541 gcgtcgctaa tatgcttgat tgattcgctc attgtatgaa tacctctatc ggttcgaggc
      601 ccgcctgaat tgctcgccac gatacgtcaa aatctaggga aacttccgtt cgctttgccg
      661 caatcaaggc cttcccggcg catgcctgaa aaaagccgaa aggctatttc cggaatgtgc
      721 ggaacgctcg cgtttgcggg tcatccgtac ggcagtttag cttaattcgc tatgcgttgc
      781 cggtggtgat agtactcatc aagggcagtg gggccaacgg ctgccggtga agcgccactt
      841 cattttgcta atcgcgacgg agcctatctg gtggctggca gcgcaaaaca caagagcgcc
      901 gaacgcaggg cgtctggagt accgactata tggtgtgaag ctcctgattc ggcgcgccga
      961 tgtcattttg gtcgtataag agtcgcgcgg tggcccaagc ggtgatagaa tgtttcgtga
     1021 cgggcctcct cgcatggagg gatggtcaac ctggtcaggt cgggaacgaa gcagccacag
     1081 ccgttttcca ccagtgccga gggtcgggct cgtcaccttc ctttttgttt gtctctgccg
     1141 tatctcccca tcttctgttt accctgggca ctgacactgc agtgtcctcg cagtggcatt
     1201 ttccgctcgc ttcatctatt tttttctatt ggtccgttag cctgtttcaa tgaagcggca
     1261 acacgcgttc gcgcgccgtt tcgattcggt tttgcgcgcc atcgttactg atacaatttc
     1321 cggatgacct atcaagttct cgcacgcaaa tggcggccga aggatttcgc ttcgctcgtc
     1381 ggacaggagc acgtggtgcg cgcgctcacg cacgcgctcg acggtggccg tctgcatcat
     1441 gcctatctgt ttaccggcac ccgcggcgtc ggcaaaacca cgctgtcgcg catcttcgcg
     1501 aaggcgctga attgcgaaac cggcgtgact tccacgccgt gcggcgtgtg ccgcgcgtgc
     1561 cgggagatcg acgaaggccg cttcgtcgat tatgtggaga tggacgcggc gagtaatcgc
     1621 ggtgtcgatg aaatggcggc gctgctggag cgcgcggttt acgcgccggt tgatgcgcgc
     1681 ttcaaggtct acatgatcga cgaagtgcac atgctgacca accacgcttt caacgcgatg
     1741 ttgaagacgc tggaagagcc gccctcgcac gtcaaattca ttcttgctac caccgatccg
     1801 cagaagattc cggtcacggt gctatcgcgc tgtctgcagt tcaatctgaa gcagatgccg
     1861 gccgggcata tcgtctcgca tctcgagcac attctcggcg aagagcaggt gccgtacgac
     1921 gcccaggcat tgcgcctgct cgcgcgcgcg gccgacggct cgatgcgcga cgcactctcg
     1981 ctgaccgatc aggcgatcgc ctattcggcc aatcaggtca atgaagaagc agtgcgcggc
     2041 atgctgggcg cgctcgatca aagctatctg attcgcctgc tcgacgcgct ggccgacggt
     2101 gacggcgccg ccgtgctggc ggtcgcggac gagatggcat tgcgcagcct gtcgttttcg
     2161 acggcgttgc aggatctggc gagcttgctg caccgaatcg cgtgggcgca gttcgcgccg
     2221 tcgtcggtgc tggacgagtg gccggaagcg ggtgatctgc gtcggtttgc tgacgcgttg
     2281 agcgccgagc aggtgcagtt gttctatcag attgtgacca tcggccgaag cgagctgggt
     2341 cttgcgccgg atgaatacgc cggtttcacg atgacgctgt tgcgcatgct ggcgttcgag
     2401 ccggcgccca cgggcggtgg tgggggcgcg gctgcggcgc gttcggcagg accggctggc
     2461 gccaggcgcg tcggttcgcc ggcggtagca gtgcagccgg cggtggcggt gcagcaggcg
     2521 gtagcagtgc agccggcggt aactgtgcag ccggaggtgg cgtcgtcgca tacggctgaa
     2581 gcaatcgccg ccgtgccggt gcgttccgca aatccgctgg cagaagcgaa gcccgcgact
     2641 ccgcgtccgc agcacctggc gtctgcgggg tctgctgaaa atgccgacac gccgaagcca
     2701 actatgcgtg aagcggcgat cgtttcgaag tccgcgtctg catcgggctc tgacgaagcg
     2761 agcgcggcgc ctgcgcccac ggcggccgag gtcgttgccg caccggcttt agcgccgtgg
     2821 caggaggaga cgtccggcca accgattctg agcgatccgg tgcctgcccc gacggacacg
     2881 agcgcgctcc gagcagcgtc gatgcccgaa gcgacgcccc cggcagccca agcaaccgaa
     2941 gcaaccgaag caaccgaagc atccgaagca tccgaagcat ccgaagcaac gaaagttgcg
     3001 cgagccctgg cgccgtggga cgacgcgcct accgagccag cgccgaacga aacacccgct
     3061 gcggccgctt ccgggcaatc ggcgccggtg tcttcgctca cggcctcggc cgcttccggg
     3121 caaccggcgg cagcgtcttc gctcgtggcc tcggccgctc cccagcaatc ggcgccggcg
     3181 tctttgctca cggctgcggc cgtagccgcg ctcgcacgtg cgcaagaggc cgcacccgca
     3241 acggcatcgc aagcaccgcc cgcagccgac atcccgccgc gccgcgcagg cggtgcgagc
     3301 gacgcgctga atgtcctgcg cagcgccggc ctgaaagtgt cgtcggagcg cggccgcacc
     3361 acggcggcag cctctgccaa gcccattgca gcccctgcca cgccgaagcc ggccgccccc
     3421 cgcgtggtcg tcaatgtgcc gacgccgggc gccgcgcgcc gcgcgccgca acaagacgcg
     3481 gcagcgcgtg cgccgtcgcc gcaggcccag cgcgacggtg tggagcaaaa cggaagcggc
     3541 tcgccgggcg cgccgtggga cgacatgctg cccgatgaat acatgccgct cacggcggcg
     3601 gacgaaggct actacggcct gccggacgac aactacatgc cggtgttcga cagcggaccc
     3661 gacgatgtcc gcatgaacgc cgcgcccgcg tcgccccccg cgccagtggt cgataagcgc
     3721 ccgctgcccg ccgccgtccc gctcgatcct ttgggtttca agggcgactg gcccgcgctc
     3781 gccgtcgatc tgccgctcaa gggtatttcg tatcaactcg cgttcaacag cgagctgatg
     3841 gcgctcgaag gcagcacgct caaactgaac gtgccggtgc cccagtacgc ggaagcctcg
     3901 caggtcgtta aactgaaagc cgcgctcgtt gaaaagctcg gccagaacct cgacgtgctg
     3961 gtcgaagtcg gcccggcgcg ccgcactgca gccgcgcacg acgccgccat gcgggcccag
     4021 cgtcagcagg aagcggagcg cgagatcggc gccgatccgt tcgtgcagtc gctcatccgc
     4081 gagttcggcg cgagcatcgt gccaggttcg atccgcccac ttacgcagga cgccgtcccg
     4141 aacggcacgc cgtccgtcca ctgattcgcc gcgcctttgc ggcgcacccg atttccggat
     4201 ttcacgctat caagaaggag catgtccatg atgaaaggtc aactcgccgg gctgatgaag
     4261 caggcccagc agatgcagga aaacatgaag aagatgcagg aacagcttgc attgatcgaa
     4321 gtcgaggggc agtcgggcgc cggtctcgtc aaggtgacga tgacctgcaa gaacgacgtg
     4381 cgccgcgtat cgatcgatcc gagcctgctc gccgacgaca aggacatgct ggaagacctg
     4441 gtcgccgctg cgttcaacga cgccgtgcgc aaggccgaag ccaccgccca ggaaaagatg
     4501 ggcggcatga cctcgggtct gccgctgccg ccgggcttca agctgccgtt ctaagcgacg
     4561 tcgccgtaag aagggaccca cgccgctgct ttgagcgcgg cgtgggttga atacgcgagt
     4621 tgcgcccgtt tagctgctag cgcctggttc atagttcata gtttgtgcaa ggttgcgtcc
     4681 gtgcagttcg gttcgtgcgt cgagcgtgcc cgcccggctc gttgccgagc taatgaacca
     4741 accacaagaa ccaaccacaa gaacgaacca caagaaccaa ccacaagaac caaccgcatc
     4801 cccacccgca tactgggcca taccgggcaa cccccacttc gacacagccg attcgcatga
     4861 aacaaccttc cgccttgtca gcgctcgtcg aagcgctgcg cgtgctgccc ggggtcgggc
     4921 ctaaatccgc gcaacgcatg gcgtatcacc tgatgcagca cgatcgcgac ggcgccgaaa
     4981 agctcgggcg ctcgttgctg ttcgcgactg aacatctgca gcactgcgag aagtgcaaca
     5041 cctttaccga agcgcggatc tgcgaggtct gcctcgatga ggagcgcgat ccggcgttgt
     5101 tgtgcgtcgt cgagacgccc gccgaccaga tcatgctcga acagacgatg acctatcgcg
     5161 gcctttattt cgtgctgatg gggcggctca gtccgctcga cggcatcggc ccgaaggaga
     5221 tccatttcga ccggctggtc aagcgcgcgt cggatggcat cgtcaaggaa gtcgtgctcg
     5281 caaccaattt caccaacgaa ggcgaggcca ccgcgcatta cctcgggcag acgctcaagg
     5341 cgcgcggtct tgccgtgacg cgcctcgcgc gcggcgtgcc ggtgggcggc gaactcgagt
     5401 acgtcgacgc cggtacgatc gcgcgtgcga tgctcgaccg gcgctcgatg tagtgcgatg
     5461 tagcatgatg taattcaatc tgcggcgtcg ctgcgtcagc aaggcccgag ccacgcacgc
     5521 gaggcctcgc tacaacgcct cgcacctcac gcccggcgcg tggccaaaca gaaacacaga
     5581 ggagacacat gagcgtcacc ccagaccctg gcgtcaccga cacacctaaa ggcccgcttg
     5641 ccggcgtcaa ggtgctcgaa ctcggcacgt tgatcgccgg cccgttcgcc gcgcgttttc
     5701 tcggcgagtt cggcgccgac gtcatcaaga tcgaagatcc caagggcggt gatccgttgc
     5761 gcaaatggcg caagctttat ccggaagccg gcggcacgtc gctatggtgg gccgttcagg
     5821 cgcgcaacaa gaagtccgtc acgatcaatc tgaaagccga agagggcaag gagatcgtgc
     5881 ggcgtctggc gaaagaagcg gatatcgtcg tcgaaaattt ccggcccgga ttgttggaga
     5941 agctaggcct cggttatgac gtcttgtcgg cggagaaccc cgggctcgtg atggtgcgtc
     6001 tgtccggcta tggccagacc gggccgtatc gcgaccggcc tggattcggc gcgatcgccg
     6061 agtcgatggg cggtctgcgc cacatcaccg gctatccgga cttgccgccg ccgcgcatcg
     6121 gcatttcaat cggcgattcg attgccgcgc tgcacggcgt gatcggcgcg ttgatggcgc
     6181 tgcatcacaa gcaggtgaac ggtggcaaag ggcaggtcgt cgacgtcgcg ttgtacgagg
     6241 cggtcttcaa catgatggag agcgtggtgc cggaatacgg cgtgtacggc atggtgcgcg
     6301 agcgcaccgg cgcgtcgctg ccgggcatcg tgccgtcgaa tacgtatcct tgccgcgatg
     6361 gcagcatcgt cattggcggc aatagcgacc cgatcttcaa gcgcctgatg atcgcgatcg
     6421 aacgcgaaga cctggcgaac gatcctgcct tggcgcacaa cgacggccgc gtgccgcgta
     6481 cccaggaaat cgacggcgcg atcgccgcgt ggctcgccac gcgcaccatc gacgaagcgc
     6541 tcgccgtgct gaacgccgcc gacgtgccgg tcggccgcat atacagcgtc gcggacatgt
     6601 tcaccgatcc gcaattcatg gcgcgccaga tgatccagcg cttcaaatgg caggacggta
     6661 aggaaatcac gctcccctcc gtgacaccca aactctcgga aactcccggc gaaacgcgct
     6721 ggttaggccc cgaactgggt gaacataccg atgaagtcct gcaatcgcta ggttatgatg
     6781 cagaccacat tgcaaggttg cacgcgcaac agatcgtgtg agtcagcgcg cggttggatc
     6841 tgctgccgcg cgtgagtaac ggctgttgca cagcagacca tgcgcgcgcc gcaagcgcta
     6901 aagcgaaccc cgatgcatcc acggcgccct cgcgcgtcgc ggcaaaaaga caacaaaagt
     6961 ctggagacaa tagaccatgc aacgcagaac cttcctcgcc ggtaccgcag cgctcgcggg
     7021 cgccacgttc gcagccacgc cgctcgcttt cgcgcagggc aaacctgaaa ccaccaaggt
     7081 ggcgatcgcc gtcggcggca agaacctgtt ctattatttg ccgctcacga ttgccgagcg
     7141 ccgcaattac ttcaaggacg aaggactcga ggtcgaaatc tcggattttg ccggcggctc
     7201 gcaagcactg aaggcagccg tgggtggcag tgcggatgtg gtgtccggcg cattcgagca
     7261 caccttgctg ttgcaggcgc agaaacaggc gtttcgtgaa ttcgtgctgc aagggcgcgc
     7321 ccagcagatc gtgctagcgg tgtcgaagaa ggcgatgccg aactacaagt cgatcgccga
     7381 tctgaagggc aagaaaatcg gcgtaaccgc gccgggttcg tcgacctcga tcatggcgag
     7441 tttcgtgctg gcgaaggccg ggttgaccgc gaaagacgtc gcgttcatcg gtgtgggcgc
     7501 aggggcgggg gcgatcgccg cgcttcagtc aggccagatc gatgcgatcg ccaatctcga
     7561 cccggtgatg accaagctcg aacgcaccgg cgagattcgc gtcgtgtcgg acacgcgcac
     7621 gcttgccgat acgcgcagcg tgttcggcgg cgatatgccg gcgggttgcc tgtatgcgtc
     7681 gcagagtttc atcacgaaga atccgaacac cacccaggcg ctgaccaatg cgatggtgcg
     7741 cgcgctcaag tggttgcaaa cggcgaccgg cagcgaactg atcagcacgg tgccggaagg
     7801 ctatctgctc ggcgatcgcg cggtgtatct ggacgcgtgg cagcacgtga aggaggcgat
     7861 gtcgccggat ggcctgatgc ccgccgacgg cccggccacc tcactgaaga ccttgcaggc
     7921 gttcgatccg aacgtgcagg gcaagccgat cgatttgtcg aagacgtgga ccaacgactt
     7981 cgtgaagaaa gcgctgacga cggtcaaggc ctgagcaccg tcagtgcggg tcgagagcgg
     8041 cacgtgccgc gttcgcctcg accaccgccg tcatgacgac atgacaaacc gaaaccgatc
     8101 ctggaagcga gtcgaatcat gaccgttgcc gccctggcgc tcgacaacat cacctgcacg
     8161 ttcgctgcgc gggacaaccg cgcgcagcgc tatacggcgg tcaaggacac gaccttgcgc
     8221 atcgcgccgg gcgaattcgt ttcggtggtc ggcccgactg gttgcggcaa atccacgctg
     8281 ctgaacgtcg gcgcgggttt gctggagccg tcttcgggaa cggtcagcgt gttcggcgac
     8341 ccgctaaagg ggatcaaccg ccgcgccggc tacatgtttc aggccgacgc cctgatgccg
     8401 tggcgctcgg ccatcgacaa cgtgatggcc ggcctggcgt ttcatggcgt gccgccggcc
     8461 gaagcgcgca gcaaggccga cgagtggttg aagcgcgtcg gtctcggcgg cttcggcgac
     8521 cgctatccgc atcaactgtc gggcggcatg cgcaagcgcg tcgcgatggc gcagacgctg
     8581 atcctcgatc cctacatcat cctgatggac gagccgtttt ccgcgctcga tatccagacg
     8641 cgtcagctga tggaaaacga attgctcgac ctgtgggccg ccaaacgcaa ggcggtgttg
     8701 ttcatcacgc acgatctaga cgaagcaatt gcga
//
LOCUS       DATASH010000735         7558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1901605,
            whole genome shotgun sequence.
ACCESSION   DATASH010000735 DATASH010000000
VERSION     DATASH010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7558
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1901605"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VHV99_19425"
     CDS             <1..204
                     /locus_tag="VHV99_19425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEX3637614.1"
                     /translation="DDATEVVPAALTALLMPFTYSIANGVAFGFISYAGLKLLTGQAR
                     KVKLVVWIIAAIFLFRYFYLGSE"
     gene            426..956
                     /locus_tag="VHV99_19430"
     CDS             426..956
                     /locus_tag="VHV99_19430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEX3637615.1"
                     /translation="MSYNRASDRYTTSAIFFHWAIFLLVALAYLAIEIRGPKGSDSRA
                     FWSNVHFWAGTLVLALAVLRLLWRLWAGAPAQIEGNRLLAFLARAAHLALYVFIFAQP
                     LLGILMTNTGGHPVTLAWANFNYTLVGADPIARPLLKTAHVWLGNAFYWVIGLHALAA
                     LAHHVVFKDRTLRRMV"
     gene            complement(1037..1327)
                     /locus_tag="VHV99_19435"
     CDS             complement(1037..1327)
                     /locus_tag="VHV99_19435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007745759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HEX3637616.1"
                     /translation="MQILFPNETPEYSGRELTLAFPAMVDWQRVECMITAEALEDHFG
                     AASPRLEDMVGAFDAHRARIEAATRRLLSETRAQCLVLRSGYVRFYEANWRN"
     gene            1746..3749
                     /locus_tag="VHV99_19440"
     CDS             1746..3749
                     /locus_tag="VHV99_19440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014971936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEX3637617.1"
                     /translation="MSHRPATPPTIGRPDVIAQAHARSLEVGLRASETPDFHPLCRPA
                     LRELVDRNQSLYTHALPVMETLHAQIVDTQSMVLLTDNHGVILHSLGDSDFVEKANRV
                     ALCPGVSWAEADRGTNAIGTALVDGQPTVVHAGEHFLHANRILTCSCAPIADPFGRTI
                     GALDVSGDTRGFHKHTLALVRMSAQMIENHLFSNQFVDAIRIHFHARAEFIGTLYEGL
                     AAFAPDGTFLSANRSALFQFGQPLAELTRQPFEALFGVALSRLLQQIARAPGENSVLT
                     LPSGVRVVARGEYAAPRYVGPVASAEGFSDAARTATSRGTRAATRPCEPAPHATLETL
                     DTGDAQVSAILRRVAKLRGRDIPILVLGKTGTGKEWLARAIHHDSPRRAAPFIALNCA
                     SLPDTLIEAELFGYEDGAFTGAKKRGSVGKIVQADGGTLFLDEIGDMPLAQQVRLMRV
                     LQERTVVPLGGTRAIPVDLRVVCATHRNLRTMIEAGTFREDLYYRINGLVVTLPALRE
                     RSDLAALIERMLALQPDGERLPRRVSGEVLERFAQCGWPGNLRQLANVLRTASIMAEG
                     TEQIELEDLPEDFLQDCVSHGGTGGVSQAGGSASRFEAARANDGAPRAADTLATPARM
                     DEWQATLIAQTLARHSGNVSAAARELGLARNTVYKYLRRSGTH"
     gene            4073..4405
                     /locus_tag="VHV99_19445"
     CDS             4073..4405
                     /locus_tag="VHV99_19445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX3637618.1"
                     /translation="MTDRPRAFSVRVEPLGQSFEAPDSLTILEAAGFANLRLPRSCRN
                     GTCRTCLCKMTAGRVRYTIEWPGLSREEKAEGYILPCVAIAETDVVIEAPDAVELQPR
                     GEAHRIQP"
     gene            complement(4402..4596)
                     /locus_tag="VHV99_19450"
     CDS             complement(4402..4596)
                     /locus_tag="VHV99_19450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HEX3637619.1"
                     /translation="MRLLIAIILPWFQFFTIGRPFAGIICLILQITLIGWIPAAIWSV
                     YALSQYNTDKKIARAMGNGR"
     gene            complement(4678..4872)
                     /locus_tag="VHV99_19455"
     CDS             complement(4678..4872)
                     /locus_tag="VHV99_19455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HEX3637620.1"
                     /translation="MVRYRHYKGGIYELVCEATLESDPTVTMIVYKAGNGTIWTRPAS
                     VFFELVDVEGVKVPRFSPIN"
     gene            5263..5700
                     /locus_tag="VHV99_19460"
     CDS             5263..5700
                     /locus_tag="VHV99_19460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX3637621.1"
                     /translation="MRVMVIVKATSESEAGKMPSTELLAAMGNFNEELVKAGVMLAGE
                     GLHPSARGKRVRFSGSQRSVIDGPFTETKELIAGFWLWQVQSMDEAVDWVRRCPNPME
                     GESEIEIRPVFEADDFGSELTPELRAQEERLRAQTEGGANPPR"
     gene            complement(5743..6387)
                     /locus_tag="VHV99_19465"
     CDS             complement(5743..6387)
                     /locus_tag="VHV99_19465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX3637622.1"
                     /translation="MRKTTVPPVKDLLLDRYAPIADGIAALFYPCAEVVIHDLRDQTI
                     AYLVNNLSKLEVGGPSVLDEVHYAARGRTIGPYEKLNWDGRRMRCVSNILFDDDGKPA
                     GMLCINFNIAVFEDVRSTLDLFIKGGNLTDAPAEELFRDDWQDRINTFLHTWLRERQI
                     GINALTREHKREIVEALHGQGAFRGRSSANYVAAVLTMGRATVYKILKQMKEGG"
     gene            6759..6977
                     /locus_tag="VHV99_19470"
     CDS             6759..6977
                     /locus_tag="VHV99_19470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637623.1"
                     /translation="MATLEAFRAVLDDKGTPEIIRNHIIDSLQYALRNHGQIFTSKEV
                     EWLAGWDDARIPLAASRELQKRVAETAR"
BASE COUNT         1373 a         2379 c         2297 g         1509 t
ORIGIN      
        1 gacgacgcca ctgaagtcgt gccggccgcc ctgaccgccc tgctgatgcc gttcacctac
       61 tcgatcgcca atggcgtcgc gttcggtttc atctcgtatg ccggcctgaa actgctgacc
      121 gggcaggcgc gcaaagtgaa actggtggtg tggatcatcg cggcgatttt tctgttccgg
      181 tatttctacc tgggcagcga gtaagcgcgc ggctcctttc tgcaccgtgc gacgccgtgc
      241 tgcgtcgtgc ggcgccgtgc aacgcggcgc ggcctgccgc aaagcatcgc ggcattgacc
      301 ctgtgtctgg ttgtggttcg gtctggatca acaatcgttt tattgaaggc gcccgttctg
      361 gtcgggcacc ctttatcatc tgatgattga taaaaaccaa cgaactggtc acaggagttc
      421 cctgcatgtc ctacaaccgc gcctcggacc gttacacgac gtccgccatc ttcttccact
      481 gggcgatctt tctgctggtc gcgctcgcct atctggcaat cgaaatacgc gggccgaaag
      541 gttccgacag ccgggctttc tggagcaacg tgcatttctg ggccggcacc ctcgtgctgg
      601 cactggcagt cctgcgcctg ctatggcggt tgtgggccgg cgcgcccgcg caaatcgaag
      661 gcaatcgcct gctcgctttt ctggcgcggg ccgcgcatct cgccctgtac gtctttatct
      721 tcgcgcagcc gctgctcggc atcctgatga ccaataccgg tgggcatccg gtgacgctcg
      781 cgtgggccaa tttcaattac acactggtcg gcgcggatcc gattgcccgg ccattgctca
      841 agacagcgca tgtgtggctt ggcaatgcgt tttactgggt gatcggattg catgcgctgg
      901 cggcgcttgc gcatcacgtg gtgttcaagg accggacgct gcggcggatg gtttgatccg
      961 cttgcggtct tgcgctcgca gcttctgaac gataccgccg caccgggtag cagcccggcg
     1021 gacggcacga cttcactcaa ttgcgccagt tcgcttcata gaaacgcacg tagccgcttc
     1081 tcaacaccag acattgcgcc cgcgtttccg atagcagacg ccgcgtcgct gcctcgatgc
     1141 gcgcgcggtg tgcgtcgaac gcgccgacca tgtcctccag acgcggcgag gcggcgccaa
     1201 agtgatcttc cagcgcttca gcggtgatca tgcactccac cctttgccaa tccaccatcg
     1261 ccgggaacgc caaggtaagt tcgcgacctg aatattcagg agtttcgttc gggaatagga
     1321 tctgcatggg agttcaccaa atgggttggg tgctgcgcat ggatcggttg gtactgcgca
     1381 cgctggttga gtctggtcgc acctgcagcg ctgtttcctc ctgtcgaatg ctggtggcgc
     1441 gggtccgtca ccccacagct gcgaacgcac tgtacttcgc gccccgcgga ggtgatttga
     1501 tccactgtag acgagttcgg gcgcggcgca agttttcctt cctgtttgct tgattttggt
     1561 gcccgacgtg gtgttttgag gcgccgttcc tctacagggc gacgcggttt gcccccgccg
     1621 cgcggctttc gccaaggtcc tgtttcgtcc tgtaatatca gcgttcggcc gcacacgcgt
     1681 atgttgcagt gcggaacgca acagcgggca acgcacccgc ccggtccgat aaccggagac
     1741 attccttgag ccatcgtcct gccacgccgc ccaccatcgg tcggccagat gtcatcgcgc
     1801 aagctcatgc ccgctcgctc gaagtcggcc tgcgggcgtc ggaaacgccg gactttcatc
     1861 cgctctgccg ccccgcactg cgtgagctcg tcgatcgcaa ccagtcgctc tatacccatg
     1921 cgctccccgt aatggagacg ctgcacgcgc agatcgtcga cacgcaaagc atggtgctgc
     1981 tcaccgacaa tcatggcgtg atcctccaca gtctcggtga cagcgatttc gtcgaaaaag
     2041 ccaatcgcgt cgcgttgtgt ccgggcgtgt catgggccga agcggatcgc ggcacgaacg
     2101 cgatcggcac cgctctcgtc gatggccagc cgaccgtcgt gcacgccggc gagcacttcc
     2161 tgcatgccaa ccgcatcctc acctgttcgt gcgcgcccat cgcggatccg ttcgggcgta
     2221 ccatcggcgc gctcgacgtc agcggcgaca cgcgcggctt ccacaaacac acgctcgcgc
     2281 tcgtgcggat gtcggcgcag atgattgaga atcatctgtt ctccaatcaa ttcgtcgacg
     2341 ccattcgcat ccactttcac gcgcgcgcgg aattcatcgg cacgctgtac gaagggctcg
     2401 ccgcattcgc gccggacggc acgttcctgt cggcgaatcg cagcgcgctg tttcaattcg
     2461 ggcagccgct cgccgagctc acacggcaac cgttcgaggc actgtttggc gttgcgttat
     2521 cgaggttgtt gcagcagatc gctcgcgcgc caggcgaaaa tagtgtgctg acgctaccga
     2581 gcggtgtgcg cgtggtcgcg cgcggcgaat atgcggcgcc gcgctatgtg ggtccggttg
     2641 cctcggcaga aggtttttct gacgcggcgc gtacggccac gtcgcgcggc acgcgtgccg
     2701 cgactcggcc ctgcgagccc gcgcctcacg ccacgctcga aacgctcgat accggcgacg
     2761 cgcaagtttc cgccatcctg cggcgcgtcg ccaaactgcg cggacgcgat attccgattc
     2821 tcgtcctcgg caaaaccggc accggcaagg agtggcttgc ccgtgcgatc catcacgact
     2881 cgccgcggcg ggccgcgccc ttcatcgcgc tgaattgcgc atcgttgccc gacacgctga
     2941 tcgaggcaga gttgttcggc tacgaggacg gcgcattcac cggcgcaaaa aaacgcggca
     3001 gcgtcggcaa gatcgtgcaa gccgatggcg gcaccttgtt cctcgacgaa atcggcgata
     3061 tgccgctcgc ccagcaagtg cgcctgatgc gcgtgctgca ggagcgtacc gtcgtgccgc
     3121 tcggcgggac gcgtgcgatt ccggtcgatc tgcgcgtcgt ctgcgcgacc caccgcaatc
     3181 tgcgcacgat gatcgaggcc ggcaccttcc gcgaagatct gtattaccgg atcaacggcc
     3241 ttgtggtcac cttgccggcg ctgcgcgagc gcagcgacct ggcggcgctc atcgagcgca
     3301 tgctggcgct gcagccggac ggcgagcgtt tgccgcgccg tgtgtccggt gaggtgctgg
     3361 aacggttcgc gcaatgcggc tggccaggca atctgcggca attggccaac gtgctgcgca
     3421 ccgcgagcat catggctgaa ggcacggaac agatcgaact cgaggatttg ccggaggatt
     3481 ttctgcagga ttgtgtcagt cacggcggca cgggcggcgt atcccaggcc ggcggaagtg
     3541 cttctcggtt cgaggcggca cgcgcaaatg acggcgcgcc gcgcgcggcc gacacgcttg
     3601 cgacgcccgc ccgtatggat gaatggcaag ccacgctgat cgcgcaaacg ctcgcaaggc
     3661 atagcggcaa cgtgtcggcg gcagcacgcg aattgggact cgcgcgcaac acggtgtaca
     3721 agtacctgcg gcgcagcggc acgcattgaa tgcgtcagca cgcgcgtcct gcgtcggtag
     3781 ctggatcggc catttcaact gcgggccgat cagcgccgcc gacgatagag gtttagaagc
     3841 tgcacgttat aaaaacggcg atccgcaaag atcgccgttt tgctttgacg tggtcacgtc
     3901 gagaagcttc tgcccggctg ccaggccgat tctacagttc gctcagcgcc gaccatttgc
     3961 cgtcttgcag cttcgaccac agtaccggcg catcggccgc tacaccgcac gcctgcccgc
     4021 caccgtgtac agtcgaagtc cgaccttttg cgcattcgca tcgaattcac tcatgactga
     4081 ccgtccacgc gctttttcag ttcgcgtcga gcccctcggc caaagcttcg aagcgcccga
     4141 ttcgctcacg atcctcgaag ccgccggctt tgcgaacctg cgtctgccgc gctcgtgccg
     4201 caacggcacg tgccgcacgt gcctgtgcaa aatgacagcg gggcgcgtgc gctacacgat
     4261 cgagtggccg gggctgagtc gcgaagaaaa agcggaaggc tatatcctgc cgtgcgtggc
     4321 gatcgctgaa accgacgtgg tgatcgaagc gccggacgcg gttgaattac agccgcgcgg
     4381 cgaggcgcat cgcattcagc cttagcggcc gttgcccatc gcgcgggcga ttttcttgtc
     4441 ggtgttgtac tggctcaacg catataccga ccagatcgcg gccggaatcc agccgatcag
     4501 cgtgatctgc aagatcaggc agatgatgcc ggcgaacggc cggccaatcg tgaagaactg
     4561 aaaccacggc agaatgatgg caatcaatag acgcattcag atttcctctt tgtatcgacc
     4621 tgacagtgcg cgttcttcgg ctacgatcgc acttgaaaat cgctcgaacg cggccgccta
     4681 attaattggt gaaaaacgcg gcaccttgac gccctcgacg tcgaccagct cgaaaaaaac
     4741 cgaagccgga cgcgtccaga tcgtaccatt gcccgctttg tagacgatca tcgtgaccgt
     4801 cggatcggac tccagcgtcg cctcgcaaac cagctcgtaa atgccgcctt tgtagtgtcg
     4861 ataacgcacc atttaactac tcctgttccg atcaacggaa tttcataaac ggaccgacgt
     4921 cctccgaagc agagcacgcg gttttgggac cgtcatttta aaccggtgcc ggcgcgcgaa
     4981 agatcacgga tgcgggcttg cgctgccgcc attcaccgtc gcgatgcacg gcggcttgag
     5041 cgacgaaaag cgccatccga ttcgggaatg cggatacagg cgtcgcgatc ttcgacgccc
     5101 caactgcctc ggctcgcggc atttgaaaag atattagagg cagtgtgctg gacgacgtcg
     5161 atttcagacg ttccggttcg tcgtagcagt aagggccaac gtgattctga atcgatttcg
     5221 gggtcgtgcg gctccccaca actcacttaa ggagaattga caatgcgcgt catggtgatc
     5281 gtgaaggcta caagcgaatc agaggccggc aaaatgccca gcacggagct gcttgcggca
     5341 atgggcaatt tcaatgaaga actcgtcaag gccggcgtga tgctcgcggg cgagggcttg
     5401 cacccgagcg cgagaggcaa gcgggtgcgt ttctcaggca gtcagcggag cgtgatcgac
     5461 ggaccgttca cggagaccaa ggaactcatc gcggggttct ggctgtggca agtccagtcg
     5521 atggacgaag cggtcgattg ggtcaggcgc tgccccaatc caatggaagg cgaaagcgag
     5581 atcgaaatcc gaccggtgtt cgaagccgat gatttcggct ccgaactgac gccggagttg
     5641 cgggcccagg aagagcgtct gcgtgcccag accgaaggcg gggcgaaccc gccgcgatag
     5701 tgcgcagggc aacagatcaa tgccaccgcg gttcgagggc cgtcagccgc cctctttcat
     5761 ctgcttcaga atcttgtaga cggtggcacg tcccattgtc agcacggcgg ccacatagtt
     5821 ggcggaactg cggccgcgga acgcgccttg cccgtgcagc gcctcgacga tttcacgctt
     5881 gtgctcgcgc gtgagcgcat tgatgccgat ttgccgttcg cgcagccacg tatgcaggaa
     5941 ggtgttgatg cggtcttgcc aatcatcgcg gaacagttcc tccgcgggcg cgtccgtcag
     6001 attgccgccc ttgatgaaca gatcgagcgt ggagcgcaca tcctcgaaca ccgcgatgtt
     6061 gaagttgatg cacaacatgc cggccggctt gccgtcgtcg tcgaacagga tgttgctcac
     6121 gcagcgcatg cggcggccgt cccagttgag cttctcatac ggcccgatcg tccggccgcg
     6181 cgccgcgtag tggacttcgt cgagcactga gggaccgccg acttcgagct tcgacaggtt
     6241 gttcacgaga tacgcaatgg tctgatcgcg caggtcgtga atcacgactt cggcgcacgg
     6301 atagaacagc gccgcgatgc cgtccgcgat cggtgcgtag cggtccagca gcaggtcttt
     6361 cacaggaggg acggttgtct tgcgcattga gaaatcgcag tgttcgaggg atgcggcgct
     6421 ggttttgccc gatgagccga aatcaactcc agtcagtcca aacggcgcgc cgaagtcatg
     6481 gccggcaagc gggacaagca caacgatgcc gcgcgcctta gcgcggcaac tcgccattgt
     6541 aacgacccca atcggcggca gcgaccacga tggcctgctg acacgcgcag cggctatttt
     6601 attgcggggc cgcatcgcgc gcggggcttt ggtgcgtgcc cgacggcata ctgcttgaaa
     6661 acgttccggt tgttgcgtct gcacccggcg atttttgttc gcttggcgag ctattttttt
     6721 tcgtgagata aaagagccac tattccatgg agcaaaagat ggccacgctc gaagcgtttc
     6781 gcgccgtgct cgatgacaaa ggcacgccgg aaatcatccg caatcacatc attgattcgt
     6841 tgcaatacgc gctgcgcaat cacgggcaga tttttacgtc gaaggaagtc gagtggctgg
     6901 ccggctggga cgatgcgcgc attccgctcg ccgcttcacg cgagctgcaa aagcgcgttg
     6961 ccgaaaccgc ccgctgaagc ccgcctgcgc ggttgcctcc gcccgcgcag ctcacttcga
     7021 tccgcacgtc agcgtccgcg ttgccggtga atttgtgcct cgcgttcgcg tttgcattca
     7081 cggcgaggcc tcccacgcgg cgtgacagtc ataccgcgag gtgccgtgcg cggcgcccct
     7141 ttcctgccca aagcccgccg gctgggcgat ttgcccgctc gccgaacttt ttgctatcca
     7201 attagcccta tttagtgcat catattggaa tagctgtatt ccgcatccgc agcaatcgac
     7261 agtaaaggcg gcaatacaag cggcagtacc aagcacagta tcgatcggcg tctccgcacc
     7321 acccccgact gttcgaccgt tgcctgtcag cgatcccgtc actccggcaa tgcgccgcct
     7381 gcattcgccg tcgcgagttg tgcagctgag ttgttcagct tttacgcgcc cgatgtccgc
     7441 gcgacatcag cgtgtttctt ctccatcgaa tccgcgactt tgcgcggtcg cgctcatgcc
     7501 cttgccatcg ggcctgtgcc aacctttttc catggcggcg cgccccaagg tgctgcgc
//
LOCUS       DATASH010000736         4946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2493772,
            whole genome shotgun sequence.
ACCESSION   DATASH010000736 DATASH010000000
VERSION     DATASH010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4946
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2493772"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(494..1897)
                     /locus_tag="VHV99_19475"
     CDS             complement(494..1897)
                     /locus_tag="VHV99_19475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX3637624.1"
                     /translation="MTRFSFRRAGRSQALVGDIAAQAGKLGIEICDVSGHVEEVAARV
                     QRQAQVCRALRESAAQTLAGNHRIAIAAREMRSVSAEAATGVQESQQTLETSLADIHG
                     LVEGVTVIESQIGALRSALAHVSRVSEEISLIARQTHLLALNAAIEAARAGDSGKSFA
                     VVAAEVKNLSAKTAQATGQIETTLAQLTQQTEQLINEGSVNTARAHRVREGTRKIGDV
                     VHATGDAITQLNEEAGQIAALTGDIETQCNGLEAQVLEMASGVEDSSENFVQAKDRLG
                     NLLGVSETLIELTAAAGVETGDTRFIEAVQHAAATVSKLFEAAVARGEISIDDLFDQN
                     YVPVSGSNPAQFMTRFTAFTDRVLPAIQEPLLGLDPRVAFCAAVDVRGYLPTHNLKFS
                     LPQRDDAVWNMANCRNRRMFDDRTGIAAASHTKKFLLQTYRRDMGGGQFVLMKDASAP
                     VFVAGRHWGGVRIGYEV"
     gene            complement(2297..2707)
                     /locus_tag="VHV99_19480"
                     /pseudo
     CDS             complement(2297..2707)
                     /locus_tag="VHV99_19480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433298.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(2820..4295)
                     /locus_tag="VHV99_19485"
     CDS             complement(2820..4295)
                     /locus_tag="VHV99_19485"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX3637625.1"
                     /translation="MTPASSAQPAAPRIAIVGSGFAGIGMAIRLQQMGITSFTVYEAA
                     GDIGGTWRDNTYPGAACDVPSHLYSFSFEPNPAWSRAFGAQAEILAYLKHCARKYGVD
                     RYVRCNVRVASCRFDEARQTWQVEIDSNGTRESIEADVVIAASGPLSRPAMPQIEGLD
                     RFEGKLFHSARWDHDYPLEGKRVAVIGTGASAIQFVPQIQPRVARLELFQRTAPWIMP
                     KPNKPIGPRAHWLFRHLPFTQRFVRNAIYWQLESRAIAFVVNPKLMKLPMKFGMSYLE
                     RRVKDPALRARLTPNYRLGCKRVLLSSDYYRALSQPNVDVVTTGIREIVTEDGAHHHA
                     DAIICGTGFQVNDVGAPFDVTGIDGADLGALWLRDGPEAYLGTSVANFPNFFMIVGPN
                     TGLGHNSMIYMIESQVQYIADCLRVLHRRKARTMNLRPNVQRDFNERLQKEMQHSVWV
                     SGCHSWYQTKSGKITALWPGFTFSFRKRTRRVRPHDYRFTP"
     gene            4440..>4946
                     /locus_tag="VHV99_19490"
     CDS             4440..>4946
                     /locus_tag="VHV99_19490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX3637626.1"
                     /translation="MKPVPAAAPATDPDAFAGQDLPPGKRKLIEAALRLTAGGRSFAS
                     LGLRELAREAGLNPNTFYRHFHTLDELAREAVESVSRRLRPMLRRERWLAAHDEPQIV
                     PRRACVAFFAFALENREAFLSALAEYHGTSPALREAVRANLHEVSAEMADDVVQLELM
                     PTLVRATVD"
BASE COUNT          944 a         1619 c         1462 g          921 t
ORIGIN      
        1 cccacgcaac accgtcctcg ccgaggcgag ccgatggcac ccgccgcgtt gaagcgaatc
       61 ccgtggtgtc ccacgcaaca ccgtcctcgc cgaggcgagc cgatggcacc caccgcgttg
      121 aagcgaagcc cgtggtgtcc cggccggacc catccgcgac gcacgcagtg cggagtggga
      181 gctgatgacg gcctggtcac taacgccgaa tgagcgatgg cacggattcc agatgcacca
      241 ctatcgcgac tgtgcggggg acccgcttat gggctagcgg tgtgagccgc tttctttgct
      301 tatctttctt tgcggcggca aagaaagtaa gtgccgcccc gcacaggggc aacgctaata
      361 gaccaatatc aattcaagga aaggccaata ccataggcac acagacacaa agcgccgcac
      421 aggcaaacag atcaaggaaa gcccaacacc ataaaagcac agacaaacaa gcgccgcgca
      481 ggcaaaaaaa ccatcaaacc tcatacccaa tccgaacccc accccaatgc cgcccggcaa
      541 caaacacggg cgccgacgca tccttcatca aaacaaactg gccaccgccc atatcgcggc
      601 gataagtctg cagcaagaac ttcttcgtat gcgatgcagc cgcaatcccg gtccggtcat
      661 cgaacatccg ccgattccgg caattcgcca tattccacac cgcatcatcg cgctgcggca
      721 aagagaactt gaggttgtga gtcggcaaat aaccacgcac atcgacagcg gcgcaaaacg
      781 ccacgcgtgg atcgagccca agcaaaggct cctgaatagc aggcaacacg cgatccgtaa
      841 acgcggtaaa ccgggtcatg aattgagcag gattgctgcc gctcacgggc acatagttct
      901 gatcgaacag atcatcaatc gatatctcgc cgcgagcaac agcggcttca aacagcttac
      961 tcaccgtcgc cgcagcgtgc tgtaccgcct cgataaagcg cgtatcccca gtttcaaccc
     1021 ccgccgccgc ggttagctcg atcaacgtct cggacacgcc gagcaaattc cccagccggt
     1081 ccttcgcctg cacaaagttc tcgctcgaat cctcgacgcc gctcgccatt tccagcacct
     1141 gtgcctcaag cccattgcat tgcgtttcta tatcaccggt cagagcggca atctgcccag
     1201 cttcctcatt gagctgtgta atcgcatcgc ccgtcgcgtg cacgacgtcg ccgatcttgc
     1261 gggtgccttc gcgtacccga tgcgcgcgcg ccgtgttcac cgagccctcg ttgatcagtt
     1321 gctcggtctg ctgagtgagt tgcgccaggg tggtctcgat ctggccggtc gcctgtgcgg
     1381 tcttggccga caggttcttc acctcggctg ccaccaccgc gaaactcttg ccggagtctc
     1441 cggcgcgcgc ggcctcgatc gcggcattta gcgccagcag atgtgtctgg cgagcgatca
     1501 gcgaaatctc ctccgagacg cggctcacgt gcgccaacgc gctgcgcagc gcaccgatct
     1561 ggctctcgat gaccgtcacg ccttccacca gcccgtggat gtcggccagc gacgtctcca
     1621 gcgtctgctg cgattcctgc acgccggtcg cggcttcggc cgatacgctg cgcatttccc
     1681 gcgcggcaat ggcaatgcga tgattccccg ccagcgtttg tgcggcggat tcgcgcagcg
     1741 cgcggcatac ctgcgcctgg cgctgcacgc gcgcggcgac ttcctcgacg tggcccgata
     1801 cgtcgcagat ttcgattccc agtttgcccg cttgcgcggc gatatcgcca acgagcgctt
     1861 gcgaacggcc ggcccggcga aagctgaagc gcgtcatgct ggtctcctga tggtgcgcct
     1921 ggcgcagtca atgagcgcgc ggatccgctg cggatgtcgc gtcctatcga cacatttacg
     1981 gcaagcttgc aaaggacttg atacggggta tcccgcagtc aatcggtatc atgagcgttc
     2041 agcttgccag tccgcagcta ctcttttctt tcaccggcaa tccatgatcg actctttcgc
     2101 gcacgtctcg ggctggcaac agctctatca tctgtgggag ctgattgtga tgctctgcaa
     2161 gtttctctgg ggggtgttgc ggttgctggg cggcgcttga aggagcgtga tgcgccggct
     2221 ccgagttggc gcatcacggc atcacggcat catggcaaca cggcatcacc tccgtcgcgt
     2281 tgcgcgtgac agaagatcag accggcgtac tgcgcgtgcc ttcggatacc ggtacacccg
     2341 ccgaatgtga gccgagaaat gcgcgcaaat cagccatcac gcgctcgggc gcttcttcca
     2401 ttggaatgtg accgagcccc ggatacatca ccgatttcgc tcccggaatg cggctcgcaa
     2461 attcggccgc atgcgccgtc ggaatccagc gatccttcgc cccccacagc accagcgccg
     2521 gcacgttcag cgtcgtcagc acgtcggtgt cgacatcctt gaaatcgagc gccggcacca
     2581 tcttgccgat cgccgtgcgc gtgccttcac cgtggaagaa ttcgacggtg cgccgcagcg
     2641 tcaccgcctc gatcttgcgc ggttcgccct acacattgcg caccgcgctc ttgataatga
     2701 cttcgggctc gcatctgctt ccgcgtctgc ctccgcattg cctcttccgc ctccgcaccg
     2761 cccgccaggc gtttcggccg ctccgtcacg cgcgcgtccc tccgccgccg catcccgctt
     2821 caaggcgtga atcgatagtc atgcggccgc acacgtcttg tgcgtttgcg aaaactgaac
     2881 gtgaagccgg gccacagcgc cgtgatcttg ccgctcttcg tctgatacca gctatggcag
     2941 ccactgaccc acacggagtg ctgcatctcc ttttgcaaac gctcattgaa gtcgcgctgg
     3001 acgttgggcc gcaggttcat cgtgcgtgcc ttgcgtcggt gcaatacgcg caggcaatcg
     3061 gcgatgtact gcacttgcga ctcgatcatg tagatcatcg agttgtgacc aaggccggta
     3121 ttcgggccga cgatcatgaa gaagttcggg aaattggcga cgctggtgcc aaggtaggcc
     3181 tccggtccat cgcgcaacca cagcgcgccg agatcggcgc cgtcgatgcc ggtgacatcg
     3241 aacggcgcgc ccacgtcgtt gacctggaaa cccgtgccgc agatgatcgc gtcggcatgg
     3301 tgatgcgcgc catcttcggt gacgatttcg cggatgccgg tcgtcaccac gtcgacgttc
     3361 ggctggctga gcgcccgata gtaatcgctc gacagcagca cgcgcttgca accgagccgg
     3421 tagttcggcg tcaacctggc gcgcagtgcg gggtccttga cgcgccgttc gaggtagctc
     3481 atgccgaact tcatcggcaa cttcatcagc ttcggattga cgacgaatgc aatggcgcgc
     3541 gattcgagct gccagtagat cgcattgcgc acgaagcgct gcgtgaacgg cagatgacgg
     3601 aacagccagt gcgcgcgcgg gccgatcggt ttattgggct tcggcatgat ccacggcgcg
     3661 gtgcgctgga acagttcgag tcgcgcaacg cgcggctgga tttgcggcac gaactggatc
     3721 gcgctggcgc cggtgccgat caccgcgacg cgcttgcctt cgagcggata gtcatgatcc
     3781 cagcgcgccg aatggaacag cttgccttcg aaccggtcaa gcccctcgat ctgcggcatg
     3841 gccggccgtg acagcggacc gctcgccgcg atcaccacat cggcctcgat gctttcgcgt
     3901 gtaccgttcg aatcgatttc gacttgccac gtctgccgcg cttcgtcgaa acggcatgag
     3961 gccacgcgca cattgcaacg cacatagcga tcgacgccgt atttgcgcgc gcaatgcttg
     4021 aggtaagcga ggatctccgc ctgggcgccg aacgcacgcg accacgccgg attcggctcg
     4081 aacgaaaacg aatatagatg cgacggcacg tcgcaggcgg cgccgggata ggtgttgtcg
     4141 cgccacgtgc caccgatatc gccggcggcc tcgtaaaccg tgaacgacgt gatgcccatc
     4201 tgctgaaggc ggattgccat gccgatgccc gcaaaaccgc ttccgacaat ggcaatgcgc
     4261 ggcgcggcgg gttgggcaga tgaggctggc gtcacagcgg gtctccggag gtggcaagat
     4321 tcgggtgtct acacatgtct acaccggcat aacgcgcagg gtagacaact gtacacttcg
     4381 cgtcaagatc gtttagagta cgactattaa acgcaacagc cgcgctctga ttgaagctca
     4441 tgaaacccgt acctgcagcg gctcccgcca ctgaccctga tgcgtttgcc gggcaggacc
     4501 tgccgcccgg caagcgcaaa ctgatcgaag ccgcgttgcg tctgaccgcg ggcgggcgca
     4561 gctttgccag cttgggtcta cgcgaactgg cacgcgaggc cggactgaat ccgaacacgt
     4621 tctaccgcca tttccatacg ctcgacgagc tggcgcgcga ggcggtcgag tcggtgagcc
     4681 gccgcttgcg gccgatgctg cggcgcgagc gctggctggc cgcgcacgac gagccgcaga
     4741 tcgtgccacg tcgcgcgtgc gtggcgtttt ttgcgtttgc attggagaac cgcgaggcgt
     4801 ttctaagcgc gctggccgag tatcacggca cctcgccggc gctgcgcgag gcggtgcggg
     4861 cgaacctcca cgaggtgtcg gcggaaatgg cggacgacgt cgtgcaactg gaattgatgc
     4921 cgacgctcgt ccgcgcaacc gtcgat
//
LOCUS       DATASH010000737        21250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5856865,
            whole genome shotgun sequence.
ACCESSION   DATASH010000737 DATASH010000000
VERSION     DATASH010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 21250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21250
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5856865"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            238..507
                     /locus_tag="VHV99_19495"
     CDS             238..507
                     /locus_tag="VHV99_19495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637627.1"
                     /translation="MKQRQLTLVAATAIALSLISGCAVADDGYFMSRHPNIEAADFNA
                     QQAIERMRAAQRANDYDMGGHAGRAIRLMQDARYEMQAAAEAATR"
     gene            655..975
                     /locus_tag="VHV99_19500"
     CDS             655..975
                     /locus_tag="VHV99_19500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007589768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4148 domain-containing protein"
                     /protein_id="HEX3637628.1"
                     /translation="MKSLIQTVAIAIAVALAFPIASFAQSNEPATRAQVQVQLAPLEQ
                     AGYDTAGDHPHDAAGIQVTETSVGQQGDISAGYGGVDGGASQSGTGIHSLNDVGLKSI
                     YIKH"
     gene            1190..2503
                     /locus_tag="VHV99_19505"
     CDS             1190..2503
                     /locus_tag="VHV99_19505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007589769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3637629.1"
                     /translation="MIKSLRNQLVLALCVLVSIVGVVQGVSSYQLSKAGLSALLDLRL
                     EQVAGRMRGGLADSLPTIPARGSQPERDIVITIWKDDQTMPYRSTEPSLALPREAPAG
                     FVTTAVGGDEWRIYTLREPSMVIQVAQRSSVRHELTQERAVNTLWPMIVLVPLVWIAV
                     LLVVRRSLRKLNRLGAQVQGIDVSHFQQLSTSGVPIEIRPFIDSINLMIDRLAQSIES
                     ERKFISDAAHELRTPLTALQLQADNLQPHIAPGNQERFRELRSGITRSGGLIAQLLRL
                     ARADAALQADTLTRVDVPAVVVDAVAEVLPIAMKRGIDIGADEMASAFVRAVEADVGI
                     AVRNLVSNAIRYTPDGGKVDLSVQKREEMVWVEVTDTGPGIAEELLPRVFDRFFRANS
                     HIEGSGLGLSIVEAIATKYGGAASLRNRRDGQSGIVASIGFPSVS"
     gene            2642..3316
                     /locus_tag="VHV99_19510"
     CDS             2642..3316
                     /locus_tag="VHV99_19510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007589771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3637630.1"
                     /translation="MRVLLVEDDLQIGQSLLRALQDADYSVDWVRDGNAGSAAIAAAE
                     YTVVLLDLGLPGMSGIDLLKASRAAGNKVPVLILTARDDLEARVQGLDVGADDYVLKP
                     FDVPELLARIRAVLRRKAGYAVSRLGDDSFNLDLDKRTLCCNGVTSVLSAREFALMLA
                     FLERPGTILSRDQLEDRLYGWGKEVESNAVDVLIHSVRKKFGQSVIRNVRGLGWTVML
                     GDSAKA"
     gene            complement(3400..3912)
                     /locus_tag="VHV99_19515"
     CDS             complement(3400..3912)
                     /locus_tag="VHV99_19515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007736385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6622 family protein"
                     /protein_id="HEX3637631.1"
                     /translation="MSPAAIIHGTPVWVWVLLVVLLSRGFKALNSGTAPLSKLAIVPL
                     IFAGWGIAHLISDPLAGWSAVIAWMTGSLVGIAGGVFIASRSRFIVDPIANTVMLPGS
                     ALPLLLIVATFVTKFWLGFEMATVTDASSLGMYVLIEVAVSGVLAGVFGGRFFTYWRA
                     MSARRVTCLQ"
     gene            4217..4999
                     /locus_tag="VHV99_19520"
     CDS             4217..4999
                     /locus_tag="VHV99_19520"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010090096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3637632.1"
                     /translation="MKSLLATLTIALLAVSSASATAKDWSTVRFGVDASYPPFESKSA
                     DGKLVGFDIDLGNEICRRLDAKCVWVENAFDGMIPALKGRKFDGVLSTMSMTPARQAQ
                     IAFSSKVFRIPTRLVAKKGSTITPTPEVLKGKRIGVEQGSIQETYAKTYWEPVGAVIV
                     SYQDQDLVYSDLIAGRIDASLQNAVQADVGFLRTPRGKDFAFAGNALYDAKTLGSGTA
                     IGLRKEDTDLKAKIDKAIADTRADGTYDRIAKKYFDFDVYGQ"
     gene            5273..5527
                     /locus_tag="VHV99_19525"
     CDS             5273..5527
                     /locus_tag="VHV99_19525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HEX3637633.1"
                     /translation="MDSDLEARIRTRAYHIWENDPSPDGNAETHWEEARRQIEAEGDD
                     TAGSPDSSADQSAERERAGRIAPEEQLQDMLRGDAGPTAH"
     gene            complement(5803..7809)
                     /locus_tag="VHV99_19530"
     CDS             complement(5803..7809)
                     /locus_tag="VHV99_19530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX3637634.1"
                     /translation="MEHAYIHLLHQLAGHPAWTLTVVFLAAFLEAIAVIGTFIPGSTA
                     MFLAGALAGTGALSLGWVFLWAIVGAIAGDGMSFWIGCRYKDRIVQLWPFRTHPQVLD
                     AGKRFFEKHGAKSVVFARFIGPLRAIVPVVAGMLGMPPLRFYAMNVLSALLWAPAHIL
                     PGVVFGASVLLAGAVSFRLVVIIALLVGIVWLSFRATGFLLSHASAWSGAAGRYLGSW
                     ACRHPGPFGRLARRLLDPQQPDATSVVVTSLIVVVSGALFFGVLEDVISGDPLVSVDL
                     SVYHFLQSVRTPWSDTVLAGLATLGSVVTLTALIVTVVIWMLLERRWRTIGYWLAAVV
                     FSQLLIFALQFAMRRAPPNELLSGAYVFPSNHVAATVIVYGFLAFLLARRVGMLEGLL
                     VATVSTVVVIVVALAGLYFGRYWVSDAIGGAALAYIWVAIVSLIAMLRHPETPPTRNF
                     MPVVILAVMLVSVGVQLSINPPAPPPDAMSRPSPVLVTEAQWTVSLWKRLPCYRSDMG
                     GDRKEPLTLQWVSNLDSIRGQLRAQGWIEGTDLSAHSLLSLASPNVAAVSLPVLPKLN
                     NGVPSSLVFMRPGDTRDERDVLRFWPSGYAVENGLSATPLWVGAFAHERLSRATWPIN
                     ILRVEKEVSSFDAHTKTSAGLGAALVARVSCHGVPVSLLSSPVE"
     gene            8082..8447
                     /locus_tag="VHV99_19535"
     CDS             8082..8447
                     /locus_tag="VHV99_19535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637635.1"
                     /translation="MKIVAKSFLVSALVIGLTGAAYAQSANMGANGMVGLQAGGINGT
                     GMGTGLGTGMGAGLGRGAASPSPMPGVGTMGHSAAIGGTGSVGSGPALNNMRANGTSL
                     NMNPDPRAPNVTGRAFGSQ"
     gene            complement(8448..9089)
                     /locus_tag="VHV99_19540"
     CDS             complement(8448..9089)
                     /locus_tag="VHV99_19540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014900740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX3637636.1"
                     /translation="MDHKLETTADAIAVSLRELIAAGELEDGARLVERELADRFGVSR
                     VPMREAIQKLEGEGLVELMRNRGAVVRTLTESDLDEIYRLRMLLEGDAIFHAVKRMDT
                     ETLSRAELVHRLLGDADTAQRQGELNREFHELLYRPCENGRQLKAIRELRAQVERYER
                     LQSTLLADTPAFQDEHLKILEACEAGNARLARSMTVEHLASAKRIVVQLIRAE"
     gene            9206..9682
                     /locus_tag="VHV99_19545"
     CDS             9206..9682
                     /locus_tag="VHV99_19545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006750183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX3637637.1"
                     /translation="MAHGEHKYAVSVQWTGNRGSGTSGYRDYGRDHIIAAGSKPEIPG
                     SSDVAFLGDANRWNPEDLLVASASACHKLWYLHLCSAAGIVVVSYRDNAEGTMLDTPA
                     QGRFAQIVLRPHVVIRAGGDVELAERLHHVAHEKCYIANSVNFPVLCEPAIATDAA"
     gene            complement(9826..11493)
                     /locus_tag="VHV99_19550"
     CDS             complement(9826..11493)
                     /locus_tag="VHV99_19550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HEX3637638.1"
                     /translation="MSTPINVSPPRSAVLDEAHLGDIKGALGTIAHHDTAPRNNWWAR
                     IRTLLAILGPGLIVMVGDNDAGAFGTYTQAGQNYGTTLLWTMLLLVPVLFVNQEMVLR
                     LGAVTGVGHARLIFERFGKFWGAFSVVDLFILNALTIVTEFIGITFVLGFFGISKVTG
                     VCIAAAVTMAAVSTGNFRRFERFAVALCLLSLLLVPVLVSIHPPVGQIARDLVMPNWP
                     VHSKLSDVMLLVIGIVGTTVAPWQLFFQQSYIVDKRITPRFMKYEKADLWIGIVFVMI
                     GAVAMISFCAALYAGKPEFGNFTDAGGVIAGLEKYAGRTPAVLFAIALLDACIIGAAA
                     VSLSTAYAIGDVFKIRHSLHRGVSDAKGFYLVYFGIVAAAAALVLIPGSPLGLLTEAV
                     QTLAGVLLPSATVFLLLLCNDRAVLGPWVNSKKLNLFTGAVVWVLVMLSIILTASVMY
                     PDITGEAIIEVLAGGTLLAVVGYAATATLRRLRPAQANAGVSAVSASEQPLSKEMRNT
                     WRMPPLGELPAPQLTLSKRVWMGVLRGYLIVAVALVIVKVVQMTLLH"
     gene            complement(11630..11797)
                     /locus_tag="VHV99_19555"
     CDS             complement(11630..11797)
                     /locus_tag="VHV99_19555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637639.1"
                     /translation="MYIAILVPPRQQTHIHSDAALALHSIKPHWLTVSWHRVKALLSD
                     PASYSGSYPAS"
     gene            12973..14445
                     /locus_tag="VHV99_19560"
     CDS             12973..14445
                     /locus_tag="VHV99_19560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HEX3637640.1"
                     /translation="MSGGPLGLTFLLSFGVAPSVLAQTLAAEQTLASDQTPAVVQAPA
                     AGQVPATEQTPAAPTGLWDRSNLLGDMGGLRPWLGNYGVTFNLEETSEYLNNLSGGAK
                     RGGAYDGLTQFGIVVDTEKALGLPGGTFNVSALQIHGSNLSQRNLLTLQSASGIEADD
                     TTRLWELWYQQSLIGGKVDVKVGLQSLDQEFMVSQFATPFINGTFGWPVLPSVDLPSG
                     GPAYPLSSLGVRLCARPSDAWTVMAGVFDGNPVGSNTGGPQQQNRPGTNFNPGGGALV
                     IGEVQYAINQPPANSSGTQATGLPGTYKLGFWYDTQNFADQRFDSTGLSLANPASNGV
                     PANHRGDYSFYAVADQTVWRRGGDSPQSVGVFARIMGAPGDRNLVDLGVNAGVTLKAP
                     FKGRDNDVAGLAVGYAKIGSHAQGLASDTASLTTPGYPSRSAETVVEATYQYQVAPWW
                     RLQADFQYVFRPAGGIPDPGNPSQRVGNEAIVGLRTNIVF"
     gene            complement(14769..15002)
                     /locus_tag="VHV99_19565"
     CDS             complement(14769..15002)
                     /locus_tag="VHV99_19565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637641.1"
                     /translation="MDHEKARAEETAAMERVLTATRRVQAAFASLQSQFPPAGTGQPS
                     QFALQTFDAALQELEDAQAGFDELLGDLLDGNR"
     gene            complement(15069..15536)
                     /locus_tag="VHV99_19570"
     CDS             complement(15069..15536)
                     /locus_tag="VHV99_19570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018425024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX3637642.1"
                     /translation="MSTNLDKTDRAILAALQNDGRMSNARLAELVGLSETPCARRLKR
                     LENDGYIDQYRAMLSRSALGLGVVAFVYVRFAVHDRAVATRFEREVQAIPRILTCHNV
                     SGSADYILQVVARDLDDYGTFMRDEMRSLPGVTSVESALSLREVKANGGLPLS"
     gene            15693..16322
                     /locus_tag="VHV99_19575"
     CDS             15693..16322
                     /locus_tag="VHV99_19575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEX3637643.1"
                     /translation="MISAHLLVVYIAALLVVYAVPGPDMALVLQTSIGRGMRSGFAAA
                     GGLGVARATHVTLSACGVAALLRSAPWLYDVVRYGGAVYLAWVGIQIFRSPGFALSEG
                     SVADVESRPLHTAFVKGLLTNLLNPKALLFCSVLLPQFVRPEAGPVALQMVELGLVLL
                     AIGACFDVMYAIGAARIAGWMRAHPLAQTLQRWTFSAALIGFALRLSLD"
     gene            complement(16618..16791)
                     /locus_tag="VHV99_19580"
     CDS             complement(16618..16791)
                     /locus_tag="VHV99_19580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018425026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HEX3637644.1"
                     /translation="MSIASPCVDICKFDGKTGLCIGCLRTRDECKSWKKMKDKHRRKI
                     IEDRSRREHKLRK"
     gene            16925..17245
                     /locus_tag="VHV99_19585"
     CDS             16925..17245
                     /locus_tag="VHV99_19585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HEX3637645.1"
                     /translation="MKTFLLYAVTAVAEIVGCYLPWRWLKEGGSVWLLVPGALSLALF
                     AWLLTLHGTAAGRVYAAYGGVYVAVAIAWLWCVDEVRPTLWDAAGVVCTLAGMAIIAF
                     QPRV"
     gene            complement(17469..17978)
                     /locus_tag="VHV99_19590"
     CDS             complement(17469..17978)
                     /locus_tag="VHV99_19590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HEX3637646.1"
                     /translation="MSISMYQASLPVLLRGMTNLQVILGKAQAHAAEKQIEPSVFTNA
                     RLAPDMLPLVRQVYIVSDTAKGCAARLAGVEAPKYDDVEQTFDELQARLQKTIDYLKE
                     FNAQQIDGSEERQITLKMRSGPIEFTGLSYLLGFVLPNFYFHVTTAYDILRHNGVELG
                     KLDYLGGIK"
     gene            18284..19840
                     /locus_tag="VHV99_19595"
     CDS             18284..19840
                     /locus_tag="VHV99_19595"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HEX3637647.1"
                     /translation="MVNRQTLPGSERTVEQGSKVVGQCDPAERIQVFVMLRRQQQAQF
                     NALMSKIEAGDPGVKPLSREAFAKDYGAAPEDIAKVKAFATAHGLTVVREDPAARQVL
                     LSGTIAQFETAFGVKLEHYQHHSAGQFRGRTGTISVPDDLHGVVEAVLGLDNRPQARP
                     HFRIRPPFQPAKGHSVSFTPLQLASLYQFPQGDGRGECVGIIELGGGYNTSDLKSYFG
                     SLGVASPTVKSVGVDQGSNQPSGDPSGPDGEVTLDIEIVGAIVPGATVAVYFTQNSDA
                     GFIDAVSRAVHDTANKPSVVSISWGAPESNWTSQSLQAFNSVLQTAAALGVTVCAASG
                     DSGSSDGSGGDQVDFPASSPYVLACGGTSLTASGTSISREVVWNDGAEGGAGGGGVSH
                     AFPVPVWQKGLSATSSGGGKKALSGRGVPDVAGDASPVTGYSVLIDGTQTVVGGTSAV
                     APLWAALIARINAAKGQPAGFINPKLYKAPGACNDITQGNNGSFAASPGWDACTGLGS
                     PNGQKVAAAL"
     gene            20189..>21250
                     /locus_tag="VHV99_19600"
     CDS             20189..>21250
                     /locus_tag="VHV99_19600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S1"
                     /protein_id="HEX3637648.1"
                     /translation="MTNTESASVSPQSSDSTHASSATAAAGTSRQAGDQPFFDPVAYG
                     NGPDDSVTDTAEAAALTHHSTAIGGHKINYTATVGHLVIVDPSSSQPDAKMFYVAFTQ
                     DNQKEETRPVTFFYNGGPGSSSVFVLLGSFAPRRIKTSMPGFTPPAPYSMEDNPDSLL
                     DKSDLVFINPVGTGYSAAIAPKKNRDFWGVDQDADSIKQFIKRFLTKNNRWNSPKYLY
                     GESYGTARSCVLAYRLHEDGVDLNGITLQSSILDYTQSGNPVGVLPTAAADAWYHKKL
                     GIAPRPTDLGAFAEEVAQFARTDYLAALRKFPTTDSATVEKLSEYTGIDKTSLLAWSL
                     DIAAYDSRGNSLFLTTLLKS"
BASE COUNT         4103 a         6373 c         6805 g         3969 t
ORIGIN      
        1 ccatgccggt ctatgcaccg ccgcctgcgc ccgtgtatta cgcgccgccg cctcccgtgt
       61 atatgccgcc cgtggtcgtt ggctattacg gacacccgcg tgggtggtac cggcactacg
      121 gatatggata cggctactgg cgctaatgag gcgtctgagc cagccgctga atcattcgtc
      181 acgggcaggc gacatcccag aacaccggac gctcatcacc tcaatggagc atgcatcatg
      241 aagcaaagac agttgacgct ggttgccgca accgccatcg cgctttccct gatctcgggc
      301 tgtgcggtcg ccgacgacgg ctatttcatg agccgtcatc cgaatatcga agccgctgac
      361 ttcaacgcgc agcaggccat cgaacggatg cgcgcggcgc aacgtgcgaa cgactacgac
      421 atgggcggcc acgctggacg cgcgatccgg ttgatgcagg acgcgcgcta cgaaatgcag
      481 gccgcagccg aagcggcgac ccgctgaccc gctgacccgc tgacccgctg acccgctgac
      541 tcatgtccag acggctgctc attcttcgct aattcttctt ctttacagtg aagtccatgg
      601 cggagaacac ctgactcacc gaatgacggg aaaacacatc tggagggaaa tatcatgaaa
      661 tcgctgatcc aaaccgttgc cattgccatt gccgttgctc ttgcgtttcc gatcgcttcg
      721 ttcgcgcagt cgaatgaacc ggcgacgcgt gcgcaagtgc aggttcaact cgcgccactc
      781 gaacaggccg gctacgacac cgccggcgac catccgcatg acgccgcggg aattcaggtt
      841 accgaaacaa gcgtcggcca gcaaggtgac atatcggccg gttatggcgg cgtcgacggc
      901 ggcgcgtcgc aaagcggcac cgggattcat tcgctgaacg acgtcggctt gaagtcgatc
      961 tatatcaagc attgagcgat tgtcaaaacc ccttctctat cgctcgttgc aacggtgggt
     1021 gcgcagcaaa ggctgcgcgc ctttgccgtc gtttcgacgc cagaaagcgc cgcgtcgcgg
     1081 gagtagaatc gctttacaaa aaaaccagtc ccacccgtct ctcgcgttgc actgagtgtc
     1141 ccggcgtcca cgcatcaccc gttggacgaa acactttttc tgcgtcccta tgatcaagtc
     1201 tctaagaaac cagctcgtcc tcgcgctttg cgtgctggtc agcatcgtcg gcgtggtgca
     1261 gggcgtcagt tcgtatcagc tgtcgaaagc aggcctgagc gcgttgctcg accttcggct
     1321 cgaacaggtc gccggccgca tgcgcggcgg tctcgcggat tcgctgccga ccattcccgc
     1381 gcgcggctcg caaccggagc gcgacattgt cattacgatc tggaaagacg atcagaccat
     1441 gccttatcgc tcgaccgaac cgtcgctggc gctgccgcgc gaggcgccgg cgggtttcgt
     1501 gacgaccgcc gtcggcggtg acgagtggcg catctacacg ctgcgtgagc cttcgatggt
     1561 gattcaggtc gcgcaacgct cgtcggtgcg gcatgagctt acgcaagaac gtgcagtcaa
     1621 tacgctttgg ccgatgatcg tactcgtgcc gctggtgtgg atcgccgtgc tgctggtcgt
     1681 gcgccgctcg ttgcgcaagt tgaatcgttt gggtgcgcaa gtgcagggga tcgacgtcag
     1741 tcattttcag caattgtcga cctcgggcgt gccgatcgag atacgtcctt tcatcgactc
     1801 gatcaatctg atgatcgatc ggctcgcaca atcgatcgag tcggagcgaa agtttatttc
     1861 cgacgccgcg cacgaactgc gcacgccgct caccgcgctt caattgcagg cggacaatct
     1921 gcagccgcat atcgcgcccg gcaatcagga acgctttcgc gaattgcgca gcggcatcac
     1981 acgcagtggc ggtctgatcg cgcaattgct caggcttgcg cgcgccgatg cggcgctgca
     2041 agccgatacg ctgacgcgcg tcgacgtgcc cgcggtggtc gtcgacgccg tggccgaagt
     2101 attgccgatc gcgatgaagc gcggcatcga tatcggcgcc gacgaaatgg ccagcgcgtt
     2161 tgtccgcgcg gtcgaagccg atgtcggcat tgccgtgcgc aatctggtca gcaacgcgat
     2221 ccgctacacg ccggacggcg gcaaggtcga tctgagcgta cagaaacgcg aggagatggt
     2281 gtgggtcgag gtgaccgaca caggccccgg cattgcggaa gaactgctgc cacgggtgtt
     2341 cgaccgcttc tttcgagcga attcgcacat cgagggcagc ggcctcggac tgtcgatcgt
     2401 cgaggcgatc gcgaccaaat atggcggcgc agcgagtctt cgcaatcgtc gtgacggcca
     2461 gtcggggatc gttgcgtcga tcggatttcc gtcagtgtct taggcctcgc gtcagttatc
     2521 atgcgttctg acccgtgccc gcgtagcggt tgcacgggcg ggctcggcct ggaatcctaa
     2581 cccgtgttcc attcctcgcg ccgcggccaa ccacaccatt cgttgctgtg gaaacgacat
     2641 catgcgagtt cttctggttg aagacgacct gcagatcggg caaagcctgc tgcgtgcctt
     2701 gcaggacgcg gactacagcg tcgattgggt gcgcgacggc aacgccggca gcgcggcgat
     2761 cgcggcggct gaatacacgg tcgtgctgct ggatctcggc ttgccgggca tgagcgggat
     2821 cgacttgctg aaagcctcgc gagcggccgg caacaaggtg ccggtgctga ttctcaccgc
     2881 gcgcgacgac ctggaggcgc gcgtgcaagg ccttgacgtc ggcgccgacg actacgttct
     2941 caaacccttc gacgttcccg aactgcttgc ccgcattcgc gcggtattgc gtcgcaaggc
     3001 gggctatgcg gtgtcgcgcc tcggcgacga ttccttcaat ctcgatctcg acaagcgcac
     3061 gctatgctgc aatggcgtga cgtcggtgct atcggcgcgc gaattcgcgt tgatgctggc
     3121 gtttctcgag cgccccggca cgatcctgtc gcgcgatcag ctggaagacc gcttatacgg
     3181 ctggggtaag gaggtggaga gcaatgcggt cgacgtgctg attcattcgg tgcgcaagaa
     3241 gttcgggcag tcggttatcc gcaatgtgcg cggcctcggc tggaccgtca tgctgggcga
     3301 ttccgccaag gcatgaggtc gtttttgccg tggatcctcg cagcttattc atgtgcgctc
     3361 ctcgaagctc tgcattcgtg cggctgaacg cgctgcgtct cactgcaggc aagtaacccg
     3421 gcgcgcgctc atcgcccgcc agtaggtgaa gaagcgcccg ccgaacacgc cagcaaggac
     3481 gccggacacc gcgacttcga tcaggacata catcccaagc gacgatgcgt cggtcacggt
     3541 cgccatttcg aacccgagcc agaatttggt tacgaacgtg gcgacaatca gcagcaacgg
     3601 cagcgcggag cccggcagca tgacggtatt cgcaatcgga tcgacaatga aacgagaccg
     3661 gctcgcgatg aacacgccgc ccgcgatgcc gacgagcgag cctgtcatcc aggcgatcac
     3721 ggcggaccat cccgccagcg gatccgaaat caggtgcgca ataccccagc ccgcaaagat
     3781 gagtgggacg atggcaagct tcgagagggg tgccgtgccg ctgttcagtg ccttgaaccc
     3841 gcgcgatagc aggacgacga gcagcaccca gacccagact ggcgtgccgt gaatgattgc
     3901 tgctggtgac atggtgagct ccttcagatc gggtgatttc tcgaggcgtg gataacgccc
     3961 gagccagtga atgacactca tcgtatcggt atggaattag cgaagaatta ggcggaacgc
     4021 gcagcgttag cgcagggtta agttgtccgc gcaacggcaa agtgcgagtt tgcacgacac
     4081 gtagtcattt atagtgtgcg ctgcacgcca cctacggctg ccgcaacgcg tcccgccgcg
     4141 ccggcgtgct gagcgccggt ttgtcgtcgt tcatttacgc cgcccatttg tttaatagag
     4201 tcataaaagg agaaacatga aatccctgct tgcaaccctg acgattgcgc tgctcgctgt
     4261 atcgtccgcc agcgcaaccg cgaaggattg gtcgaccgtg cggttcggtg tcgacgcgag
     4321 ctatccgccg ttcgaatcaa aatccgccga cggcaagctc gtcggcttcg acatcgacct
     4381 gggcaatgag atctgccgcc gcctggacgc aaaatgcgta tgggtcgaaa acgccttcga
     4441 cggaatgatt ccggcactga aggggcgcaa gttcgacggc gtgctatcga ccatgtcgat
     4501 gacgccggcg cgtcaggcgc agatcgcgtt ctcgtcgaag gtattccgta tcccgacccg
     4561 cctcgtcgcg aagaaaggct cgaccatcac gccgacgccg gaagtgctga agggcaagcg
     4621 catcggcgtc gagcagggct cgatccagga aacgtatgcg aagacctact gggagccggt
     4681 cggcgcggta atcgtgtcct atcaggatca ggatctcgtt tactcggacc tgattgccgg
     4741 ccgcatcgac gcatcgctgc agaacgccgt gcaggccgac gttggtttct tgcgcacgcc
     4801 gcgcggcaaa gactttgcat ttgccggcaa tgcgctttac gacgcgaaga cgctgggaag
     4861 cggcaccgcc atcggtttgc gcaaggaaga caccgacctg aaagcgaaga tcgacaaggc
     4921 gatcgccgac acgcgcgcgg acgggacgta cgacaggatc gcgaaaaagt atttcgactt
     4981 cgacgtctac ggtcagtgaa aacgggccgg ccgacgcgca acgcgtgcgg cctcaggctt
     5041 tagcgaaggc ggccagagca cccgcgggcg atcagaacga cgccggttgc ccggcgcgcg
     5101 gcatcagccc agcggcgctc ggcggttgat cttgagcttt tcgcgccgag ccacgcgtag
     5161 aacaagtacg cgcaaacgtg gcatgccgga tgctgaggaa atgtgcgaga cggcgcggcc
     5221 cgattgttcc gatggtcccg agcgtcgcgc gcatttcacg gagaaacgga acatggacag
     5281 cgatctcgaa gcccgcattc gcactcgggc gtatcacatc tgggaaaacg acccgtcgcc
     5341 ggacggcaat gcggaaacgc actgggagga ggcgcgcagg cagatcgaag cggaaggcga
     5401 tgatacagcg ggtagtcccg actccagcgc cgaccagtcg gcggaacggg agcgcgcggg
     5461 caggattgcg ccggaggaac aattgcagga catgctgcgc ggcgatgcgg gcccgaccgc
     5521 tcactgaacc cagcaaagcc cgggcaggcc aatcagcttc ggtggcctgc gccggcaccg
     5581 cccactggcg gtcagatttc ctcgacatca ggcgaacgcg tgcccggctt cggcttggac
     5641 ggatcgcatg gcccggaggt tatccacaga aaatgttcat atccttgtgg acaagtcctg
     5701 gccaattccg ccaagtgatt gagggcaggg cgatttcctg atcggcatgg cgaacacgca
     5761 cagccacgca gccaaccacg cagacgcgcg gcgacacgcc gctcattcca ccggagagga
     5821 cagcagcgat accggcacgc catggcaact taccctggcg acgagcgccg cgccgaggcc
     5881 ggcgctcgtt ttcgtatgag catcgaacga cgacacttcc ttttcgacgc gcagaatgtt
     5941 gatcggccag gtggcccggg agagccgctc gtgcgcgaac gctccaaccc acagcggcgt
     6001 ggctgacagg ccgttttcca cggcatagcc gctcggccag aagcgcagga cgtcgcgttc
     6061 gtcgcgggtg tcgccgggac gcatgaagac cagcgacgaa ggcacgccgt tgttcagctt
     6121 cggcaatacc ggcagcgaca ccgccgcgac attcggcgag gcaagcgaga gcaggctgtg
     6181 cgcggacagg tccgtgcctt cgatccaccc ttgggcgcgc aattgccccc tgatcgaatc
     6241 gaggttcgac acccattgca gcgtaagcgg ttccttgcgg tcgccgccca tatcggagcg
     6301 atagcagggg agtctcttcc acagggacac cgtccactgc gcttctgtga ccagcacagg
     6361 cgacgggcgc gacatggcat ccggcggcgg cgccggtgga ttgatgctca gttgcacccc
     6421 cacgctcacg agcatcaccg cgagaatcac gaccggcatg aagttgcgtg tgggcggcgt
     6481 ttccggatgc cgcaacatcg ctatcaacga gacgatggcc acccagatat acgcgagcgc
     6541 ggcgccgccg atcgcgtcgg agacccagta ccggccgaag taaagtcccg ccagcgcgac
     6601 cacgatcacg accaccgtgc tcacggtcgc caccagcaaa ccttccagca tgccgacgcg
     6661 ccgcgcgagc aggaacgcga gaaagccgta gacgatcacc gtcgcggcga cgtgattgct
     6721 cgggaacacg taggcgccgg acaacagttc attgggcggc gcgcggcgca tcgcgaattg
     6781 caaggcgaag atcagcagtt gggaaaatac gaccgcggcg agccagtaac cgatggtgcg
     6841 ccaacgccgc tccaacagca tccatatcac cacggtcacg atcagcgccg tcagcgtgac
     6901 gacgctgcca agcgtggcca atcctgcaag cacggtgtcg ctccagggcg tgcgcaccga
     6961 ttgcaaaaag tgatagacgg acagatcgac gctgaccagc ggatcgccgc tgatcacgtc
     7021 ttcgagaacc ccaaagaaga gcgcgcccga caccaccacg atcagcgacg tgacgacgac
     7081 gctggtggca tccggctgtt gagggtctag cagccgccgc gcgagacggc cgaacgggcc
     7141 cgggtgacgg cacgcccagc ttcccagata gcggccggcc gcgcccgacc acgcactggc
     7201 atgcgagagc aggaaacccg tcgcgcgaaa gctcaaccag acgatcccca ccagcaacgc
     7261 aatgatgacg acgaggcgga acgacacggc gccggccagc agcaccgagg cgccgaatac
     7321 cacgccgggg aggatatgcg ccggcgccca tagcagcgcc gacaacacgt tcatcgcgta
     7381 gaagcgcagc ggcggcatgc ccagcatgcc cgccaccacc ggcacgatcg ctcgcaatgg
     7441 tccgatgaag cgggcgaaca cgacgctctt ggcgccgtgc ttctcgaaga agcgtttgcc
     7501 ggcgtcgagt acctgcggat gagtgcggaa cggccacagc tggacgatcc tgtccttgta
     7561 gcggcagccg atccagaagc tcatgccgtc ccccgcgatc gcgccgacga tcgcccacag
     7621 gaacacccag ccgagactca gcgcacctgt gccggccagc gctccggcga gaaacatggc
     7681 agtgctgccc gggatgaagg tgccgatgac ggcgatggcc tcaaggaagg cagcaagaaa
     7741 gacgaccgtg agcgtccacg ccggatggcc ggcaagctgg tgcaaaaggt ggatgtaggc
     7801 gtgctccatc gtgcgcgatc ggccgttctg gcgtaacggt tgatcataac gcgcacggcc
     7861 ttgcccgata ttcctgcaaa ccagtagccg gtggcatgcc ggcgcgaagc ctcacgtcca
     7921 aacgccgatc gtcgatcaaa ccctgaattc agcgatgaac aaacggggcc ggctgccggt
     7981 ctgactattt gaccggtttc cttcacgagc gtcgttagtt cgacatcgcg acggtggttt
     8041 gagtaattcg gtcgacaatt tgatgcatga cggagtggac tatgaagatc gtggcaaaga
     8101 gttttttggt gtcggcactg gtaatcggtc tgacgggtgc ggcttatgcg cagtcggcca
     8161 acatgggcgc caatggtatg gtcggtttgc aggcaggcgg gatcaacggc acgggcatgg
     8221 gaacggggct ggggacggga atgggggcgg gtctgggcag gggagcggca agtccgagcc
     8281 cgatgccagg cgtgggtacg atgggccact cagctgcgat cggcgggacc ggcagcgttg
     8341 gctcgggccc ggcgctcaac aacatgcggg cgaacggcac cagcctgaac atgaatccgg
     8401 atcctcgcgc accgaatgta acgggtagag cgttcggatc gcagtaatca ctcagcccga
     8461 atcagttgca caacaatgcg ctttgccgat gcaagatgtt ccactgtcat tgatcgcgca
     8521 aggcgtgcgt tgccggcctc gcaggcttcg agaatcttca gatgctcgtc ctggaaggcc
     8581 ggcgtatcgg cgagcagcgt gctttgcaat cgttcgtaac gttcgacctg agcgcgcaac
     8641 tcgcggatcg ctttcaattg ccggccgttc tcacacgggc ggtatagcaa ttcatgaaac
     8701 tcgcgattca gttccccctg tctttgcgcc gtatcggcgt cgcccagcag ccgatggacg
     8761 agttcagcgc gcgataacgt ctcggtgtcc atgcgcttga ctgcgtggaa gatggcgtcc
     8821 ccttcgagca acatgcgcag gcggtagatc tcgtcgaggt cggactcagt cagggttcgc
     8881 actacggcgc cgcgattgcg catgagttcg accaagcctt cgccctcgag cttttgtatg
     8941 gcctctcgca tcggcacccg gctcacgccg aatcgatcgg cgagttcgcg ctcgaccagc
     9001 cgcgcaccgt cctccagttc gccggccgca atcagttcgc gcagtgagac ggcgatggca
     9061 tcggcggttg tttccagttt gtgatccatc tgcggtgagt cctcgggtat gggtcgtttg
     9121 gaataatggt ataccaaact ggtgtgtgcg tatagaatgc atgtgtggtt aattcgaatc
     9181 cgtggttaat ggaaaggagt ttggcatggc gcacggcgag cacaaatatg cggtatcggt
     9241 gcagtggaca ggcaaccgcg gcagtggcac gtcgggatat cgtgattacg gtcgggacca
     9301 catcatcgcg gcgggaagca agcccgagat tcccggatca tcagacgtgg cgtttctcgg
     9361 cgacgccaac cggtggaatc cggaagacct gctggtcgcc tcggcatccg cctgccacaa
     9421 gctctggtat ctgcatcttt gctcggccgc gggcatcgtt gtggtgagct atcgcgacaa
     9481 cgccgaaggg acgatgctcg atacgccggc gcaggggcgc tttgcgcaga tcgttctgcg
     9541 tccgcacgtc gtcattcggg ccggcgggga tgtcgagctg gccgagcgtt tgcaccatgt
     9601 cgcgcacgag aagtgctata tcgcgaactc cgtcaacttt cctgttttgt gcgagccggc
     9661 aattgcgacg gatgcagcgt gacggacggg cggcggtcac cacgcatccg gtagagagat
     9721 cgcaacacaa ctggtcgggc aaagccgcgt ggcgtgtttc gcgtgaagat cgaccgttgc
     9781 ccgccgctca ccgggaacaa ccggaagcgg acgggcagcg ttgatttaat gcagcaacgt
     9841 catctgtacg accttcacga tgacaagcgc aacggccacg atcagataac cgcgcagcac
     9901 gcccatccag acgcgtttgg acagggtcag ttgcggcgcc gggagttctc ccagcggtgg
     9961 catgcgccac gtattgcgca tttctttcga aagcggttgc tcgctagccg aaaccgcgga
    10021 aaccccggca ttcgcttgtg cgggccgcag cctgcgaagc gtcgcggttg cagcgtagcc
    10081 gacaacggcc agtagcgtgc cgcccgccag gacttcgata atcgcttcgc cggtaatgtc
    10141 cggatacatc accgacgcag tcaggatgat ggagagcatg acgagcaccc agaccactgc
    10201 gccggtgaaa aggttgagct tcttcgaatt cacccacggg ccgagcacgg cgcggtcgtt
    10261 gcacaacagc agcagaaaca ccgtcgcgct cggaagcagc acaccggcga gcgtctgcac
    10321 cgcttcagtc agtagtccga gtgggctgcc gggaatcaat accagcgccg ccgcggccgc
    10381 gacaatgccg aagtagacaa ggtagaagcc ctttgcgtcc gatacgccgc ggtgcagcga
    10441 gtgacgaatc ttgaacacgt cgccaattgc gtaagcggtg gacaacgaga cggccgccgc
    10501 gccgatgatg caggcatcga gcaacgcgat cgcgaagaga accgcgggtg tgcggccggc
    10561 atatttttcg aggccggcga tcacgccgcc cgcatccgtg aaattgccga actccggttt
    10621 gcctgcgtac aacgcggcgc agaacgagat catggccacc gcaccgatca tcacgaatac
    10681 gatgccgatc cacaggtcgg ctttctcata cttcatgaag cgcggcgtga tgcgtttatc
    10741 cacgatatag ctctgctgga aaaacagctg ccacggcgca accgtggtgc cgacaatgcc
    10801 gatcacgagc aacatcacat cgctcagttt cgaatggacg ggccagttcg gcatcaccag
    10861 atcacgcgcg atctggccga cgggcggatg aatcgacacg agtacgggga cgagcagcag
    10921 gctcaacaga cagagcgcga ccgcgaagcg ctcgaagcgg cggaagttgc ctgtactgac
    10981 cgcggccatc gtcaccgccg cggcgatgca gacgccagtc actttcgaaa tgccgaagaa
    11041 accgagcacg aacgtgatgc cgatgaactc ggtcacgatg gtgagcgcgt tcaatatgaa
    11101 caggtcgacg acgctgaatg cgccccagaa cttgccgaag cgttcgaaga tcagtcgcgc
    11161 atggcccacg cccgtcaccg caccgaggcg cagcaccatt tcctggttga cgaaaagaac
    11221 gggcacgagc agcagcatgg tccacagcaa ggtggttccg taattctgtc ctgcctgcgt
    11281 gtaggtgccg aaggcgccgg catcgttgtc gccgaccatc acgatgagac ccggcccgag
    11341 gatcgcgagc agcgttcgga tgcgggccca ccagttgttg cgcggtgcgg tgtcgtgatg
    11401 cgcgatggtg ccgagcgcgc ctttgatgtc gccgagatgc gcttcgtcga gaacggcgct
    11461 gcgtggtggt gagacgttaa ttggagtgga catttttagc cgcctgagta ggtgtgattt
    11521 ttgtgcaatg gcgcgtcttc gggatccgct gcgcgagtgt cgtcgaatgg gggcgagcga
    11581 cggcgtcgca tgcggattgc gaaggcgtgc gggtgctgac agtgcgtaat tacgaagctg
    11641 ggtacgaacc tgagtacgaa gccgggtccg agaggagcgc tttgacgcgg tgccaggaca
    11701 cggtgagcca gtgcggcttg atgctgtgaa gcgcgagtgc agcgtcgctg tggatatggg
    11761 tttgctgacg gggcggtacg aggatggcga tgtacatggt atttctccgt gcgtatggct
    11821 ctacgcggat tgcagaaagc cagcagacac ggagcgcggc cggcactatg cgcgcggcgg
    11881 cgaatgtcta ctcgctttcc tcaggcaaaa gaaagattgc gtggtaattg agcggcataa
    11941 gggataactg tcactgtccg gcatggcggc tccttatcgg tgttgcttat cggcgttgag
    12001 tacgcacgtg tttgtccacg cgctcggccg cgccggaatt cgaacggatc gaaccggcgt
    12061 gagcgaacgc agccgcgagt ggatgctcgc gacgcgatgc cattcggacg tatgggcgca
    12121 cggacgcagg acatgcgtcg agcgcggcgc ggccgaatgg cgctagttaa ggtgcacgga
    12181 gagagttact acggcgcgca ccgtctgcct gctggcaaga cggcggctaa ggaaagagag
    12241 cgcgggcgtc tggctggtca ggcagtgaag ctgttcgaaa atcgactact gctggtgtcc
    12301 aaggcaatgg ccccgtttgt gataacgggc gaattttagg cactcccgca agcagaagtc
    12361 aacatgcttc gttaagcgaa gcaaatattc cttttttatg tgttgtggcg taccaattct
    12421 tcttcaccag agtgggatga gtctttcgga aatgacagga aagacaggta gatgaagggc
    12481 gcaagtgcag cggtgaatag cgcagcctga tgtctgcgaa ggcgcggccg caaattcgga
    12541 gtgcgatttc tttaatgcgt ttttacttgc gattttatga gtgtgccgaa tacaattcgc
    12601 ccgtctcagc aaaaggagtc cctcgtggac acatgctcta gtagtggaag tccgatgccg
    12661 gtctacgccg gcgcaatgtc cacgagttag ccgcaggatc actccttcgc cgctaactcg
    12721 ctccgttcgg gttagcgcgc ttcttcagtg accggttcct gccggtctgc cagtcgcccg
    12781 ctccctcagt ttgatcgtaa tcgcatcgtg tgcgagaccc ggtcgcgcct tcgtacatcg
    12841 cccggcgtgg ccgggctcga tgggagtgag cttatgttcg tcaaatcgtc gtcgtatcgt
    12901 tctttggcgt gccgcgtgcg cggcgctcaa tctcgtctcg cgcagaaaaa ccccgcaaaa
    12961 accgggtggt ggatgtccgg cggtcccctc ggtctaacct tcctgttgtc gtttggcgtc
    13021 gcgccctcgg tgctggcaca gacgctggcc gcggaacaaa cgcttgcttc ggatcagaca
    13081 cctgccgtag ttcaggcgcc tgccgcggga caggtgcctg ccactgagca gacacctgcc
    13141 gcgcccactg ggctctggga ccgctcgaat ctgctcggcg acatgggcgg gctgcgtccc
    13201 tggctcggta actacggggt gaccttcaac ctggaggaaa ccagcgagta tctgaacaac
    13261 ctgtcgggtg gcgctaaacg gggcggcgct tacgatgggc tcacccagtt cggtatcgtt
    13321 gtcgatacgg aaaaagcgct gggactgccg ggcggcacgt tcaatgtgtc ggcgttgcag
    13381 attcacggca gcaatctgag ccagcggaat ttgctgaccc tgcaaagcgc gagcggcatc
    13441 gaggccgacg atacgacgcg gctgtgggaa ctctggtatc agcaatccct gatcggcgga
    13501 aaagtggacg tgaaggtcgg gctacagagt ctcgaccagg aattcatggt gagccagttt
    13561 gcgacgcctt tcatcaacgg aactttcggc tggcctgtat tgccgtcggt ggacctgccg
    13621 tcgggtgggc ccgcttatcc attgtcgtcg ctgggtgtgc gattgtgtgc cagaccttcg
    13681 gacgcatgga ccgtgatggc cggcgtcttc gacggcaatc cggtgggcag caatacgggc
    13741 ggcccacagc aacagaaccg tcccggcacg aatttcaatc cgggcggcgg cgcgttggtc
    13801 atcggcgagg tgcagtacgc aatcaaccag cctccagcga actcgtcggg tacgcaggca
    13861 accggtttgc ccggtaccta caagctgggc ttctggtacg acacgcaaaa cttcgcggac
    13921 cagcgtttcg acagtaccgg tttgtcgctg gcgaacccgg cgagcaacgg cgtaccggca
    13981 aatcaccggg gcgactacag cttctacgcg gttgccgacc agacggtctg gcggcggggc
    14041 ggggacagcc cgcagtcggt gggcgttttc gcgagaatca tgggcgcgcc gggcgatcgc
    14101 aatctggtgg atctgggcgt gaatgcaggc gtgacgctca aagcgccgtt caaagggcgc
    14161 gacaacgacg ttgccggatt ggccgtcggc tacgcgaaga ttggctcgca tgctcaaggg
    14221 ctggcaagcg ataccgcctc gctgaccaca ccgggttatc cgtcgcgcag cgctgagact
    14281 gtcgtggagg cgacttacca gtatcaggtc gcgccgtggt ggcggttaca ggcggacttc
    14341 caatatgtct tccgtccggc gggtggcatt ccggatccgg gcaatccgtc gcagcgcgtc
    14401 ggtaacgaag cgatcgtcgg attgcggacc aatattgttt tttgagtgga cgggcggaat
    14461 tgaggagggc gattctgcaa gcaacagcgt attgaaacgc ctggttcgcg ggccggtcgg
    14521 ttgatcgggc ccggacctgc ggatgctgct gcggacgacc agacgatgat gccgttagac
    14581 ggctgacata ttcacttttg gttctggcgt cggcggggcg gtttcacctc aagctatgaa
    14641 tgtgcaacta acggtcaagt ccgccccgcc tctccaaata gcgcgacgac cgtctcgata
    14701 gaaactgaag acggccaccg ctcgcgtact acgcagcagc tatccaatga agagaccggc
    14761 tatgccactc aacgattgcc gtcaagcaga tcgccgagca attcgtcgaa tccggcttgg
    14821 gcgtcttcga gttcctgcaa ggcagcgtcg aaggtttgca gcgcgaactg cgaaggctgg
    14881 ccggtgccgg ccggcggaaa ttgcgattgc agtgacgcga aggccgcctg cacccgcctg
    14941 gttgcggtca atacgcgctc catcgcggct gtttcttcgg cccttgcctt ttcgtgatcc
    15001 atgaaaaact ccgtggtcgt ctggtcggct cgcgccgtat caaatgggtg actatgccac
    15061 gtccgcgttc aggacagcgg cagcccgccg ttagccttca cctcccgcag ggacaacgcc
    15121 gattccactg acgtcacgcc cggcaggctg cgcatctcgt cgcgcatgaa agtgccgtag
    15181 tcgtcgagat cgcgcgcgac cacctgcagg atgtagtcgg cgctgcccga cacgttgtga
    15241 cacgtcagaa ttcgcgggat tgcctgcact tcgcgctcga aacgggtcgc caccgcgcgg
    15301 tcgtgcacgg caaaacgcac gtagacaaac gcgaccacgc cgaggccgag ggcggagcgc
    15361 gaaagcatcg cacggtactg gtcgatatag ccgtcgttct ccaggcgctt gaggcggcgc
    15421 gcgcaaggcg tctcgctcag gccgaccagt tcggccaagc gtgcattcga catgcggccg
    15481 tcgttctgca acgcggcgag gatcgcgcga tctgttttat cgaggttggt tgacatgact
    15541 ttggggtgct tgctatgggt tagcggaaaa catgggtggg gagtgaaatg ttagcgtatt
    15601 cctccacaag tggagtcgta gccccctgta tttcgccagt aaaccctcca tcgacgatag
    15661 caaaatagag gcctcgaaaa taagaggctt gtatgatttc cgcacatctt ctggtcgtct
    15721 atatcgctgc gttgttggtg gtttatgccg tgccgggccc cgacatggcg ctggttctac
    15781 agaccagtat cgggcgcggt atgcgcagtg gcttcgcggc ggctggcggg ttgggcgtgg
    15841 cgcgtgcgac gcacgtcacg ctgtcggcat gcggagtggc ggcgctgttg cgcagcgcgc
    15901 cctggctata cgatgttgtg cgttatggcg gcgcggttta tctggcgtgg gttggcatcc
    15961 agatcttccg ctcacctggg ttcgcgctgt ctgagggcag tgtggcggat gtcgagtcgc
    16021 ggcctttgca taccgctttc gtcaagggct tgctcaccaa tctgttgaac ccgaaagcgc
    16081 tgctgttctg ctcggtgcta ctgccgcagt tcgtgcgtcc tgaggccgga ccggttgcgc
    16141 tgcagatggt cgaactgggt ctggtgctgc tcgccatcgg cgcgtgtttc gacgtcatgt
    16201 acgcgatcgg cgcggcgcgc atcgccggct ggatgcgagc tcatcctctc gcgcagacac
    16261 tgcagcgttg gactttctcc gcggcgctga tcggcttcgc gctgcggctc tcgctggact
    16321 gatgtgctgc ggtactgtgg tactgcggta ttgccgtgaa gatggcgcgt cggtttcggg
    16381 tcgcaccggt tctgattggt tcgcaacgtt cgcaccgttt cggttctcat cggttcgcac
    16441 cgttttccgc cgattcgccc cggctcgatt ggttcctatc cggtcccacc gattcgcatc
    16501 ggcttgtatc gttcgcaacg gcgaacagcg ctcctgcgtg gccgcgcctt attcgccgca
    16561 caagcgatga tcgcttaacc atctgggcgg tccgttccca tgatccggcg cggctgctca
    16621 cttacgcaat ttatgctccc ggcgtgaacg atcctcgatg attttccggc gatgcttgtc
    16681 tttcatcttt ttccagcttt tacattcgtc gcgagtgcgc agacagccga tgcaaaggcc
    16741 ggttttaccg tcgaacttgc agatgtcgac acagggtgac gcgatactca tgacagtcgg
    16801 ggaatagcgg tagttgcgac gactgtcgca tgcgtcatgc ccgcgtgcaa agtgattgtg
    16861 ttaataaagc cgatagagtt gcgcacagct gcgcggcaat acaaccactc gcttcgatcg
    16921 ttcgatgaaa acttttctgc tttatgccgt gaccgccgtt gccgaaatcg ttggctgcta
    16981 tttgccctgg cgttggctaa aggagggcgg ctccgtctgg ctgctggtgc ctggcgcgct
    17041 cagcctcgcc ttgttcgcct ggctgctgac cctgcatggc accgccgcag gccgcgtata
    17101 cgcggcttac ggcggtgtgt atgtggcggt ggctatcgcg tggctatggt gcgtcgacga
    17161 agtgcgcccc acgctgtggg atgcggccgg cgtggtgtgc acgctggccg gcatggcgat
    17221 catcgcgttc caaccgcgcg tttgatgctc ttcgcgcatg gcctgtgcgt ggctttgtgc
    17281 ttggggcatg cgcggttcat gggccgcttt gcgcaacgcg cgacgttcac gtgtggctcg
    17341 tgcgtgagtt cacgtgtggc tcacatgcga gcgcagtgtg agccacacgc ggcttgtgaa
    17401 ccacctgtga cccgcgtgtt gcgggagccg cattcctgcg ccgctcacgc attagcacgc
    17461 ccgcgtgctt acttgatacc gcccagatag tcgagcttgc cgagttcgac gccgttgtgc
    17521 cgcagaatgt cgtacgccgt cgtgacgtga aaatagaagt tcggcagcac gaaaccaagc
    17581 agatacgaca gccccgtgaa ttcgatcggg ccgctgcgca ttttcagcgt gatctgacgt
    17641 tcttccgatc cgtcgatctg ttgcgcattg aattccttca gataatcaat cgtcttttga
    17701 aggcgggcct ggagttcgtc gaaggtctgt tcgacgtcgt cgtacttcgg cgcttcgacg
    17761 ccggccagac gcgcggcgca gcccttcgcc gtatcgctca cgatatacac ctggcgcacc
    17821 agcggcagca tgtccggcgc gagccgggcg ttcgtgaaca cggacggctc gatctgtttt
    17881 tcggcggcgt gcgcctgggc cttgccgaga atgacttgca ggttggtcat gccgcgcaac
    17941 agcacgggca atgatgcttg atacatcgaa attgacatgg gttcctctga ttctccgatc
    18001 gtgcgcgccg gtggcgcgcg aggcgctgca acggctggat tgccgcttca gcatcatagc
    18061 gcggccgctg cacgatgcgc attgatccaa ccaggcgtta gccattcgga caactgccgc
    18121 gcgcaccgga actgtgttcg aatgcatggg tcggacgatt tggcgcagtc cttgaaacaa
    18181 cccgccgccg cggatcgcgc ggcggtgagt tcgtgcaggc gcgtgccgtg cctggagtcc
    18241 gcctcgcggc tcgcgccgtc acgctaacag gagaatcgac accatggtca acagacaaac
    18301 gctgccggga agcgagcgca cggtagagca aggttcgaaa gttgtcgggc aatgcgatcc
    18361 ggccgaacgg atccaggttt tcgtgatgct gcgccggcag cagcaggcgc agttcaacgc
    18421 actgatgagc aagatcgaag cgggcgaccc gggcgtcaaa cccctttcgc gcgaggcgtt
    18481 cgccaaagat tacggcgccg cgccggaaga catcgccaag gttaaagcct ttgccaccgc
    18541 acacggcttg acggtggtac gggaggatcc agcggcgcgc caggtgttgc tgagcggcac
    18601 gattgcgcag ttcgagactg ccttcggcgt gaagctggaa cattaccagc atcacagcgc
    18661 gggacagttc cgtggccgca ccggcacgat cagcgtgccg gacgatctgc acggcgtcgt
    18721 ggaggccgtg ctcgggctcg ataaccggcc tcaggcgcgg ccgcactttc gcatccggcc
    18781 gccgttccag ccggccaaag ggcattcggt gtcctttacg ccgctgcagc tcgcatcgtt
    18841 gtatcagttt ccgcaaggcg acggcagggg cgaatgtgtc ggcatcatcg aactcggcgg
    18901 cgggtacaac acgtccgacc tcaaaagtta ctttgggagc cttggggtcg cctcgccaac
    18961 ggtgaaatca gtgggcgtcg atcagggcag caatcagccg agcggcgacc cgagcggtcc
    19021 cgacggcgaa gtcacgctcg acatcgaaat cgtcggtgcg atcgtgcctg gcgcgacagt
    19081 cgcggtctat ttcacacaga acagcgatgc cggtttcatc gacgcggtga gccgagcggt
    19141 acatgacacg gccaacaaac cgtcggttgt ctcgatcagt tggggcgccc cggagtcgaa
    19201 ctggaccagc cagtcgctgc aagctttcaa cagcgtgctg caaactgcag cggctctcgg
    19261 tgtgactgtt tgcgcggcgt ctggcgatag cggctcgagc gatggctccg gcggcgatca
    19321 ggtcgacttt ccggcgtcga gcccttatgt gctggcatgc ggcggcacga gcctgacggc
    19381 gtcgggcaca tcgatctcgc gtgaggtggt gtggaacgac ggcgcagagg gcggcgcggg
    19441 cggcggtggc gtgagccacg cctttccggt gccggtctgg caaaagggcc tgtcggcgac
    19501 ttcatcgggc ggcggcaaga aggcgctcag cgggcgcggc gtgccggacg tggccggcga
    19561 tgcgtcgcct gtcaccggct atagcgtatt gatcgacggt acgcagacgg tggtcggcgg
    19621 caccagcgcg gtcgcgccgc tgtgggcggc actcattgcg cggatcaatg cggcgaaagg
    19681 gcagccggcc ggtttcatca acccgaagtt gtacaaggcg ccgggcgcgt gcaacgacat
    19741 cacgcaaggc aacaacggca gcttcgccgc gtcgccgggc tgggatgcat gcacgggtct
    19801 cggcagcccc aatggtcaga aggtggcggc ggcactatag tctgatgagc cgcgggtcgg
    19861 ttcggggcga gcttggcgga ctgtcggctg aggcgatcgc gggccggccg tgactggtcc
    19921 caaggtgcgc cgcgttcagg ccgcaggggt agagtgcatg gcttcagggg atgcaggcat
    19981 tggcattgca ggtgacgcag gcacgcagag caacgcaggc acgcagagca acgcaggcac
    20041 gcaggcacgc aggcacgcag agcagcgcag gcacgcagag cagcgcaggc acacagagca
    20101 acacaggcac gcagagcacc gccggcaccg caggacaccg cgagacacac acgagacacc
    20161 aagacgttaa cgaacccatg gagcgatcat gacgaacacc gagtcagctt ccgttagtcc
    20221 gcagtcgtca gacagtacgc atgcttcgtc cgcaacggct gccgccggca cgtcgcgtca
    20281 agccggcgat cagccgttct tcgacccggt tgcttacggc aacggtccgg acgactccgt
    20341 gaccgacacc gctgaagcag ccgcgctcac gcaccattcg accgcgatcg gtggccacaa
    20401 gatcaactac acggcgacgg tgggacacct cgtaattgtc gacccgagca gttcgcagcc
    20461 cgacgccaaa atgttctacg tcgcgttcac gcaggacaac cagaaggagg aaacccgccc
    20521 ggtgactttc ttctacaacg gcggtcccgg ctcgtcttcg gtgttcgtgc tgctgggctc
    20581 gttcgcgcca cgccgcatca agacgtcgat gccgggcttt acgccgcccg caccctactc
    20641 gatggaagac aacccggaca gcttgctcga caagagcgat ctcgtcttta tcaatccggt
    20701 cggcaccggc tattcggcgg cgattgcgcc gaaaaagaac cgcgacttct ggggcgtcga
    20761 ccaggacgca gactcgatca agcagttcat caagcgcttt ctgaccaaaa acaatcgctg
    20821 gaattcgccg aagtatctgt acggcgagtc ttatggcacg gcgcgcagct gtgttcttgc
    20881 gtaccgtttg cacgaagacg gcgtcgacct gaacggcatc acgctgcaat cgtcgattct
    20941 cgattacacg caatccggca atccggtcgg cgtgttgccc accgccgccg cggacgcgtg
    21001 gtatcacaag aaactcggca tcgcgccgcg gccgaccgat ctcggcgcgt tcgccgagga
    21061 agtcgcgcag ttcgcgcgca ccgattatct ggcggcgctg cgcaagtttc cgaccaccga
    21121 ttcggcgacc gtcgaaaagc tgagcgagta caccggcatc gacaaaacaa gcctgctcgc
    21181 gtggagcctg gacatcgcgg cctacgacag ccggggcaat tcgctgttcc tgaccacgct
    21241 gctgaagtcg
//
LOCUS       DATASH010000738        21811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_515697,
            whole genome shotgun sequence.
ACCESSION   DATASH010000738 DATASH010000000
VERSION     DATASH010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 21811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21811
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515697"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..1736
                     /locus_tag="VHV99_19605"
     CDS             159..1736
                     /locus_tag="VHV99_19605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018432234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX3637649.1"
                     /translation="MTSRLPPKVSAADFDHALAGWRAVVGEPQVVTSAAGLAAYLDPF
                     APGERAAFSASAALLPASVDEIRAVLRIANQFRIPLWTVSTGRNFAYGGAAPRLPGSV
                     VLDLQRMNRIIEVNETLAYALVEPGVSYFDLYAHLREKGYKLWVDPPAAGWGSVVGNT
                     LERGFGYTAYGDHAATQCGMEVVLANGDVLRTGMGAIEIGTAWQLYQPGYGPSFDAMF
                     MQSNYGIVTKLGVWLMPAPPAYLLGEIQFQHEEDLETIVDILRPLRLDETIRNNAVIE
                     GGLRRAAGLSARRQWYEGKGAMPESAVAAMLDKLNVGRWNLHFALYGTPELIDARYAI
                     VQRAFARVPKAKLSAARYTGDAQPTAGGDRNLAGIPAMSAFRMLDWRGGAGAHVDFAP
                     LCPATGRDAQRQYTMVKARAAEYGFDYYGGFTAGVRHLHHIFAAIFDRDDANQVEQAG
                     ALLRSLMSDARAAGYGEYRAHLAYMDFAAAQYSFNDGALLRLSETIKDALDPNGILAP
                     GKQGIWPAAWRDRRGYT"
     gene            1794..2210
                     /locus_tag="VHV99_19610"
     CDS             1794..2210
                     /locus_tag="VHV99_19610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637650.1"
                     /translation="MDRRTFVITSAWLSTAAGGAWPWLAQAAARRDTLAVIDSTLAGG
                     PAFADYVASMKLPAFEAGDDIGMLWYSTLAPRLSQTPAQAPASLIGLTRVSDYFVLRE
                     LATRVGHMVEHSHEQGTGPGAQVAHIAFALTPRLVR"
     gene            complement(2207..3187)
                     /locus_tag="VHV99_19615"
     CDS             complement(2207..3187)
                     /locus_tag="VHV99_19615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1571 domain-containing protein"
                     /protein_id="HEX3637651.1"
                     /translation="MRLARFSPETATLSASLTASLRAISSRGLLIGALVASVAAVATP
                     AAFAQNDDTPPALDTASAVKAPATPSSQVGKLTLDQQVKWLRAAQQSGAMEKLDDAQL
                     VALFQSLDPLALPRYIKEGPNGYPSYEFTMARSERIHGQWPDKPDHMLGRIAHDPLRI
                     YLKWLPDGAHAGQEIIYDESKRPDEMYGHLGGIMNVMPLWTSLNGALARAQSNHQVRD
                     LGTEYIANQYLSEGRKYIEAGLPRSTKVEVQTIDGVRVVTFTFETPTGQPQFYAKKET
                     LGLDLRHPYFRTVESYDNDGRIFEKIVFESITPKTFDDSTFDPKNKAYQF"
     gene            complement(3355..5184)
                     /locus_tag="VHV99_19620"
     CDS             complement(3355..5184)
                     /locus_tag="VHV99_19620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX3637652.1"
                     /translation="MTQTTQTPLSPAAGSTPPANAAGQAPAQTPGQAPERAITQTPRT
                     DGIWYASYPADVPHEIDVNQYDSIVQFFDECIAQFRERVAYVSVGANLTYGELGRKAT
                     AFAAYLQSIGVKPGERVAIMLPNTFQYPVSLFGVLKAGGVVVNVNPLYTVRELAHQLK
                     DSGAQTIIVFENFAKTVEDALPGTKVQNVIVTGLGDLLADGLNLKGRLLNFMLRHVKK
                     MVPEYNLPKAVPLLEALSIGYRRPLTPVRPTHDDIAFLQYTGGTTGVAKGAMLTHRNI
                     IANLLQAKAWSEGQLSGDIETVLTPLPLYHIYSLTVNALIFMGLGGRNILIANPRDMK
                     RVMMIIRHEKFTGMTAVNTLYNAFLDNEEFCKRDFSDLKLAMAGGMATQKSVADRFKA
                     VTGKPIIEGYGLTECSPIVSMNPVDLTHLRDFEGSIGLPAPSTQVRFRKDDGTWANIG
                     EAGELCVKGPQVMKGYWNRPDETAKVIDDDGWLATGDIGVMDSRGYIRLIDRKKDMIL
                     VSGFNVYPNEVEDVIAALPDVREVAAIGVPDAAQGERVKVFIVKRNPALSAEQVIAHC
                     RKNLTGYKVPKLVEFRDELPQTNVGKILRRALRDEELAKQKPA"
     gene            complement(5240..6541)
                     /locus_tag="VHV99_19625"
     CDS             complement(5240..6541)
                     /locus_tag="VHV99_19625"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018432230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX3637653.1"
                     /translation="MSNPLPAVRRVAIIGGNRIPFARSNTAYATASNQDMLTFTLQGL
                     IDRYNLHGERLGEVAAGAVIKHSRDFNLTRESVLSTTVARETPAYDVQQACGTGLEAA
                     ILVANKIALGQIEVGIAGGVDTTSDAPIGVNERMRKILLEANRGKSTGQRVGALTKLR
                     PGMFFKPLLPRNGEPRTGLSMGEHCELMAKRWNISREAQDVLAYDSHRKLTEAYARGF
                     VNDLMTPYRGLARDNNLRADLTLEKLAGLKPVFDRDAGTLTAGNSTPLTDGASAVLLA
                     SDAWAARHDLPVLAYLSWSETAAVDFFDKKEGLLMAPAYAVPRMLARAGLTLQDFDLY
                     EIHEAFAAQVLCTLAAWQDDEYCRTQLGMNGPLGAIDRAKLNVNGSSLATGHPFAATG
                     GRIVAGLAKMLAQLDKPAGTARGLISICAAGGQGVVAILER"
     gene            complement(6743..7654)
                     /locus_tag="VHV99_19630"
     CDS             complement(6743..7654)
                     /locus_tag="VHV99_19630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX3637654.1"
                     /translation="MGEPFRSGHAASAGRPKTVVIEQLPAPAKLYARALSGIVKRGRD
                     THLPALRLARPAVALDPGPISRYSRVCGFIPEHGVPLTYPHLLAFPLHLLMLTDPAFP
                     WPALGLVHLANHVRLRRPLAYRDLLRVEVEFGALLRHDKGQAFVLHTRMYRRGEAVWD
                     GESVYLKRGVPASGSPLDPLELGHDPLQRISRWQLPPQLGRDYARVSGDYNPIHLTAL
                     SAKAFGFPRAIAHGMWTLARAAAALQPPKPLAEAALSAEFKLPMLLPGEASLWSASPS
                     LIERDLEVRDITGDKPHLRGRMQWRLL"
     gene            complement(7657..9069)
                     /locus_tag="VHV99_19635"
     CDS             complement(7657..9069)
                     /locus_tag="VHV99_19635"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEX3637655.1"
                     /translation="MNDSYLNFVNSPFGARLARSLGLPKPEVLRRYRADQPEFDGLIA
                     IGAGREPHLLDALANLVNSIGVTSVAHESAGLWVPLANRHGLMTGRFEPAESGSHGKL
                     TALLFDASGIEDSSQLEPLHGFFHDTLRSLGKCGRIIVLGRPPESCATPRQWTAQRAL
                     EGLTRSLGKEARRGITANLVYVEQGAENGIEATLRFFLSPRSAYVSGQVVRIGVRAGA
                     DALPAFDWNQPLAARRAVVTGAARGIGASIASVLAAEGAHVIGIDIPSARDALDATMR
                     QLNGIALAFDIATPEAPAQIAAALDEQGVDIVVHNAGITRDKTIAKMTDAAWQSVIDI
                     NLSAQERIDDALLAAGILRDGGRIIGVSSISGMAGNLGQTNYATSKAGVIGRVQSMAP
                     HLRARGITINAVAPGFIETQMTAKIPLTLREAGRRMNSLSQGGQPVDVAQTIAWLAHP
                     GAAGVSGQVVRVCGQSLIGA"
     gene            complement(9218..11716)
                     /locus_tag="VHV99_19640"
     CDS             complement(9218..11716)
                     /locus_tag="VHV99_19640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX3637656.1"
                     /translation="MPCFILVLVIAALALVYVQARASWWFGFMIVWVAAAHLSGAAGA
                     VATTLLAIVFVLPALVLTIKPLRRAWLTKPVLDIFRTILPEMSATERDAIEAGTVWWD
                     AQLFSGRPHWDTLLGYGPATLTAEEQSFLDVECEHLCDLANDWETTTVWQDLSPNTWQ
                     YIKERGFLGMIIPKQYGGKQFSAYAHSQVIMKLATRCSAAAVSVMVPNSLGPAELLMH
                     YGTEEQKNHYLPRLARGDEIPCFALTSPYAGSDAAAIPDLGIVCKGAFEGRETLGFRV
                     TWDKRYITLGPIATVLGLAFRALDPDHLLGSNDEPGITCALIPTDHPGVNIGRRHWPL
                     NAVFQNGPNSGKDVFIPLDWVIGGRAQVGNGWRMLMECLAAGRAISLPSSNVGMAKIA
                     VRGTGAYAAIRRQFRTAVGKFEGVQEALGRMGGNLYVMDAARRLSAHAVDLGEKPSVI
                     SAIAKYHITERARMVINDGMDIAAGKGICMGPSNFLARAYQQVPIAITVEGANILTRC
                     LIIFGQGAIRCHPYVLKEMAATRETDRARALRDFDAAFFGHVSFTLSNAVRSFVYGIT
                     GGAFIARPRMAYTPLLSYYSAATRLSTAFALLADVSMFVLGGDLKRRERISGRLGDVL
                     SQLYLISATLKRFEDEGRQEEDLPLVRWGVEDSLYKAQHALDGVLANYPNRLAAGLVR
                     VLAFPFGLPYREPSDRLGSEIAELMQTPGAARERLVSDSYVPHPDVDALGYGELVFEL
                     NPRFTEIEQKLREAVKQGLLEPMPQSLLQLAAWTEAAQHKGLIDTDDRRVLDDYARYG
                     AEVVKVDDFPADFDMLANLQQRKDALEKALELAA"
     gene            complement(12046..12912)
                     /locus_tag="VHV99_19645"
     CDS             complement(12046..12912)
                     /locus_tag="VHV99_19645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX3637657.1"
                     /translation="MAVRTTGRHAGDTKSRILDAAETLFIDCGYEAMSLRQITSRAEV
                     NLAAVNYHFGSKESLIHSMLSRRLDRLNQERLKLLDRFDHMLGARLTCEHVLGAMFIP
                     ALRLSREPRVGGKAFLRLLGRAYTDPSAFIRDFLNAHYASVAVRFFDAFQRALPHLPR
                     EELGWRLHFANGALSGVLAGTDTDSLISEFSQGKSMNDLQLTARLASLMVAALKAPLP
                     DGSQLATFAAVLGDATESGPESQVEGMNPAAGTQQASGGFESSSPAVSDGERRGGNME
                     ACAFQPALHGTA"
     gene            complement(13270..14490)
                     /locus_tag="VHV99_19650"
     CDS             complement(13270..14490)
                     /locus_tag="VHV99_19650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3637658.1"
                     /translation="MSWTREQRNVTIAAYLGWTLDAFDFFLMVFVLKDIAAEFNTKIP
                     EVAFGIMLTLVMRPVGALIFGWLADKYGRRPTLMVNIACFSLLELLSGFSPNLATLLV
                     LRALFGIAMGGEWGVGGALTMETVPPKSRGIVSGLLQAGYPSGYLLASIVFGVFYQYI
                     GWRGMFFVGVLPALLVLYVRAHVPESPAFKTLEKKARPGLIATLKQNVGLSLYSIVLM
                     TAFNFFSHGSQDLYPTFLRVQHGFDAHTVSLITITLNIGAICGGLFFGYMSERIGRKR
                     AIFIAALIALPVLPLWAFSSTPVLLAVGAFLMQISVQGAWGVIPVHLNEISPDEIRAT
                     FPGLVYQLGNLIASVNGPLQASAAEAHGNNYALIMAVVIGIVAVVIAALIPFSRERRG
                     IDMTQSAKQVAAQL"
     gene            14984..16315
                     /locus_tag="VHV99_19655"
     CDS             14984..16315
                     /locus_tag="VHV99_19655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEX3637659.1"
                     /translation="MSFKSITSEPVRHEQRPLAVLAAVTLLTGIGAGLGGMLLALLLH
                     CIQHLAYGYSVTHVISSESFLQGVTDAEPERRVFVLTICGLVAGLGWWLLYRFGRPLV
                     SIRQAVKSDDPRMPLLSTTVHALLQIVTVALGSPLGREVAPREIGSMLAGWLSHRAGL
                     SVEQNRIMVACGAGAGLAAVYNVPLGGAVFVLEVLLGTFGWKAAVPAVVTSAVAALVA
                     QAGLGNEHQYLVPPLTVDTSLMVWAVVCGPIFGIAAWGFTEITKRARADAPKDWRLPV
                     LSLLNFAIIGVLAMHFPQLLGNGKGPAALAFDGDLTVSLAAMLLVLKAVITAGSLRAG
                     AEGGLLTPGLANGALLAIVLGGAWSLFWPGASFGAFAVVGATAFLAASMQMPITAVVL
                     VFEFTRVSHDFLIPVLFAVGGAVLSFQGCVKWAPALQFGSGFNWSGAGKAR"
     gene            complement(16385..16615)
                     /locus_tag="VHV99_19660"
     CDS             complement(16385..16615)
                     /locus_tag="VHV99_19660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HEX3637660.1"
                     /translation="MPIVTIQVTREGTRPGVDSVTAEEKAELIRGVSHLLLDVLNKPL
                     EATFVVIQEVETENWGWGGLPVDEYRKQRGLK"
     gene            complement(16729..17439)
                     /locus_tag="VHV99_19665"
     CDS             complement(16729..17439)
                     /locus_tag="VHV99_19665"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3637661.1"
                     /translation="MSTSPKVVVVTGASQGIGAEVVKAFRKLDYRVVATARSIKPSED
                     PNILTVAGDIADPATARRVISEAVKRFGRIDTLVNNAGVFIAKPFTRYTAEDYATITG
                     VNLNGFFYITQLAIAEMEKHSSGHVVSVSTTLAEHAIDGVPSVLASLTKGGLNAATRS
                     LAIEYAKRGIRANAVSPGIIKSPMHAVETHAALGALHPLGHMGEMSDIVNAIVYLDSA
                     PFVTGEILHVDGGQSAGH"
     gene            17825..18256
                     /locus_tag="VHV99_19670"
     CDS             17825..18256
                     /locus_tag="VHV99_19670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637662.1"
                     /translation="MRRLLLAAFAATFFVTCVHAQSNAFGPLVTPSGNLQFVRVDRDF
                     VGMLDKEIFDRFGASTLTHFDDVGNAGDTVTRMLVQTDAGLVLYDFRHRPPLVQRSDK
                     RMMVKRVFWQDDEVVMQSSQGWFRFKRGVLTKLQSSTTTYH"
     gene            18468..19148
                     /locus_tag="VHV99_19675"
     CDS             18468..19148
                     /locus_tag="VHV99_19675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3637663.1"
                     /translation="MNEPSPSIEIETAPNPGFAVILMHGLGADANDFVPLVPELRLTD
                     APAVRFVFPNAPEIAVTANDGYVMRAWYDIRSFQDVNRQVDEAGIESSCATVRRLIEA
                     QNQRGIPTSNIFLAGFSQGAAMTYSAGLTHPEALAGLIVMSGYVPSRGFVDSRLSAAN
                     SSTPIFAAHGLYDDVLPIQLGEAARDFAIAKGCNVDWNAYPMPHSVCEEEISALRVWL
                     GALLTASS"
     gene            complement(19325..20206)
                     /locus_tag="VHV99_19680"
     CDS             complement(19325..20206)
                     /locus_tag="VHV99_19680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637664.1"
                     /translation="MIEPHVTHSLLRRQPYNLQHDCRLLDKVHPTRAIERGHFHMDHY
                     VFSAFVLVLLLSSAALGSYVRSRLADHHFEEASLAAMRIAVGLVATLSALVLSLLISS
                     GKSSLDLVNTALQHNAVTMIRLDRTLSQFGPSADGLRLQIKNDYARWISFLFSNKTGT
                     TAEAETREILQSTYDIQGRIFAFQPLGASQERLRDRAMELWDEIFAGRWLAFEHRKGL
                     IPAPLTVVLVGWLAVIFGIFGFSAPRNWSMCVVFFLCAASATTAVFVVLDLDTPFQGM
                     VNVSKTPMLDALNFIGK"
     gene            complement(20203..20331)
                     /locus_tag="VHV99_19685"
                     /pseudo
     CDS             complement(20203..20331)
                     /locus_tag="VHV99_19685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903731.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
     gene            21110..21265
                     /locus_tag="VHV99_19690"
     CDS             21110..21265
                     /locus_tag="VHV99_19690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637665.1"
                     /translation="MKLITGGLGIGPGLVQNVEREGLLEESRSASAAVEDRAVVLADG
                     GAPWSRH"
     gene            complement(21372..21458)
                     /locus_tag="VHV99_19695"
     tRNA            complement(21372..21458)
                     /locus_tag="VHV99_19695"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21422..21424),aa:Leu,seq:cag)
     gene            complement(21722..21808)
                     /locus_tag="VHV99_19700"
     tRNA            complement(21722..21808)
                     /locus_tag="VHV99_19700"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21772..21774),aa:Leu,seq:cag)
BASE COUNT         3978 a         6577 c         6959 g         4297 t
ORIGIN      
        1 gcgaccttga gccgcagcgt gccagcagcc gtagagagac gaccttgatc cgcagcttgc
       61 cagcagccgt agagaagcga ccttgatccg cagcttgccc gcagccatag aggagcgacc
      121 ttgacacaca gcttgcccgc agccatagag gagcagcttt gacaagccgc ttgccgccga
      181 aagtttccgc cgccgatttc gaccacgcgc tcgccggctg gcgcgcggtt gtcggtgagc
      241 cgcaggtggt gacgtcggcc gcggggctcg ctgcgtatct ggatccgttc gcgcccggcg
      301 agcgcgcagc gttctccgcg tccgccgcgc tgctgcccgc ttccgtcgac gagattcgcg
      361 cggtgttgcg gattgccaat caatttcgga ttccgctgtg gacggtctcg accggacgca
      421 acttcgcgta cggcggggcc gcgccgcggc tgccgggttc agtggtgctg gatctgcaac
      481 ggatgaaccg catcatcgaa gtgaacgaga cgctcgccta cgcgctggtc gaaccaggcg
      541 tcagctactt cgatctgtac gcgcatctgc gtgagaaagg ctacaagctc tgggtcgatc
      601 cgcccgcggc ggggtggggc agcgtggtcg gcaatacgct cgaacgcggc ttcggctaca
      661 ccgcgtatgg cgatcatgcc gcgacgcagt gtggcatgga agtggtgctc gccaacggcg
      721 acgtgctgcg caccggaatg ggcgctatcg aaatcggcac tgcgtggcag ctctatcagc
      781 cgggttacgg gccttcgttc gatgcaatgt tcatgcagtc gaattacggc atcgtcacca
      841 agctcggcgt gtggttgatg cctgcgccgc ctgcctatct gctgggcgaa atccagtttc
      901 agcatgaaga ggatctcgaa acgatcgtcg atatcttgcg accgttgcgg ctcgatgaaa
      961 cgatccgcaa taacgcggtg atcgaaggcg gtttgcgccg ggctgcggga ttgtcggcac
     1021 gtcggcagtg gtacgaaggc aaaggcgcga tgccagaaag cgcggtagcg gcaatgctcg
     1081 acaagctgaa tgtgggccgc tggaatctgc atttcgcgct atatggcacg cctgaactga
     1141 tcgacgcacg ctacgcgatc gtgcagcgcg cgttcgctcg cgtgccgaag gcgaaacttt
     1201 cggcggcgcg ttatacgggt gatgcgcaac ccacagcggg cggcgatcgc aacctggccg
     1261 gcatcccggc gatgagcgcg tttcgcatgc tcgactggcg cggcggcgcg ggcgcgcatg
     1321 tggacttcgc gccgctgtgc ccggcgaccg gccgcgacgc gcagcgccaa tacacgatgg
     1381 tgaaggcgcg cgcggcggaa tacgggttcg attactacgg cggttttacg gcgggcgtgc
     1441 gtcatctgca tcacatcttc gccgcgatct tcgaccgcga cgacgccaat caggtcgagc
     1501 aggccggcgc tttactccgt tcgctgatga gcgatgctcg cgcggcgggt tacggtgaat
     1561 atcgcgcgca tctcgcgtat atggatttcg ccgcggcgca gtacagtttc aacgacggcg
     1621 cattgctgcg gctctcggaa acgatcaagg acgcgctcga cccgaacggt attctcgcgc
     1681 cgggcaagca ggggatctgg ccggcggcgt ggcgcgaccg gcggggttat acgtgagggc
     1741 gtgtgacccc aataccgtgc atgccccgca tcatctaatc acgtgatgaa cccatggacc
     1801 gacgcacttt cgtcataacc agcgcatggc tttcgaccgc cgcaggtggg gcgtggccgt
     1861 ggctcgcgca ggcagccgct cgccgcgaca cgctcgccgt gatcgattcc acgcttgcgg
     1921 gcggcccggc atttgccgac tacgttgcca gcatgaagct gccagccttc gaagccggcg
     1981 acgatatcgg catgctctgg tacagcacgc ttgcgccgcg tctcagccag acgcccgcac
     2041 aagcgccggc gtcgctgatc ggcctcacac gcgtatccga ttacttcgtg ctcagagagc
     2101 tggccacccg cgtaggccac atggtcgaac acagccacga gcagggcacg gggccgggcg
     2161 cgcaggtcgc gcatatcgcc ttcgcgctga cgccgcgcct tgtgcgctag aactgataag
     2221 ccttgttctt cggatcgaag gtcgagtcgt cgaacgtttt gggcgtgatg ctttcgaaga
     2281 cgatcttctc gaagatcctg ccgtcattgt cataagactc gacggtgcgg aaatacggat
     2341 gccgcagatc cagcccgagc gtttctttct tcgcgtagaa ctgcggctgg ccggtcggcg
     2401 tttcgaaggt gaaggtgacg acgcgcaccc cgtcgatcgt ttgcacttcc accttggtcg
     2461 agcgcggcaa gccggcctcg atatattttc tgccttcgga aaggtactga ttggcaatgt
     2521 actcggtacc gagatcgcgc acctgatggt tcgattgcgc gcgggcgagg gcgccgttca
     2581 gcgaggtcca cagcggcatc acattcatga tgccgccgag gtggccatac atttcgtcgg
     2641 ggcgtttgga ctcgtcgtag atgatttcct gacccgcatg cgcgccatcg ggcagccact
     2701 tcaggtagat ccgcagcggg tcgtgcgcga tgcgccccag catgtgatca ggcttgtcgg
     2761 gccactggcc gtgaatgcgt tccgagcgtg ccatggtgaa ctcgtacgat ggataaccgt
     2821 tcggcccctc cttgatgtaa cgcggtaagg caagcggatc gagcgactgg aacagcgcga
     2881 ccagttgcgc gtcgtcgagc ttctccattg cgccgctttg ttgcgcggcg cgcagccatt
     2941 tgacctgctg atccagcgtg agcttgccga cctgcgagga cggcgtggca ggcgccttga
     3001 cggcgctcgc ggtgtctagc gcgggcggtg tgtcgtcgtt ctgcgcgaaa gccgctggcg
     3061 ttgccaccgc cgcgacgctg gccactagcg cgccgatcag aagtccgcgc gatgaaatcg
     3121 cccgcaatga agctgtcagg gacgcgctca acgttgcagt ctccggcgaa aaacgggcga
     3181 gacgcatgaa ggccggcgag cggttcgagc cgctcgaccg gggcaagcgg ctcaagcgac
     3241 ccgatcggca caagcggtac aagctaccca agctacccaa gcgtgcggac atgctgtgct
     3301 tcatgaagct ctccgaaagg atggcgccag gcatggtgtc gagcgccctg cctgtcaggc
     3361 aggtttttgc ttcgcgagtt cctcgtcgcg cagcgcgcgg cgcagaatct tgccgacgtt
     3421 ggtttgcggc aactcatcgc ggaactcgac gagcttcggc accttatacc ccgtgaggtt
     3481 cttgcggcag tgggcgatca cctgttcggc gctcagcgcc ggattgcgct tgacgatgaa
     3541 tactttcacc cgttcgcctt gcgccgcgtc gggcactccg attgctgcga cttcgcgcac
     3601 gtccggaagc gctgcgatca cgtcttcgac ttcgttcgga tagacgttga agcccgacac
     3661 caggatcatg tccttcttcc ggtcgatcag gcggatgtag ccacgcgaat ccatcacgcc
     3721 gatgtcgccg gtggcgagcc agccatcgtc gtcgatcacc ttggcggttt cgtccggacg
     3781 attccagtag cctttcatca cctgtggacc cttcacgcac agttcgccgg cttcgccgat
     3841 gttcgcccac gtgccgtcgt ctttcctgaa gcgcacttgt gtggacggcg cgggcaaacc
     3901 gatcgagcct tcgaagtcgc gcagatgggt caggtccacc ggattcatcg acacgatcgg
     3961 cgagcactcg gtcagcccat agccttcgat gatcggtttg ccggtgaccg ccttgaaacg
     4021 atccgcgacc gacttctgcg tggccatgcc acccgccatc gcgagcttca ggtcggagaa
     4081 gtcgcgcttg cagaactcct cgttgtcgag gaacgcgttg tagagcgtgt tcaccgccgt
     4141 catgccggtg aatttctcgt gccggatgat catcatcacg cgcttcatgt cgcgcggatt
     4201 ggcgatcagg atgttgcgtc cgccgagtcc catgaagatc agcgcgttca cggtcagcga
     4261 atagatgtgg tacagcggca gcggcgtgag caccgtttcg atgtcgccgc tcaattggcc
     4321 ctcggaccac gcctttgcct gcaacagatt ggcgatgatg ttcctgtgcg tgagcatcgc
     4381 gcctttggct acgccagtgg tgccgccggt gtactgcagg aaggcgatgt cgtcgtgagt
     4441 ggggcgcacg ggcgtcaagg gccgcctgta gccgatcgag agcgcttcga gcagcggcac
     4501 ggctttcggc aggttgtatt ccggcaccat cttcttcacg tggcgcagca tgaaattgag
     4561 cagacgtccc ttcagattca gaccatcggc gagcagatcg ccgagcccgg tcacgatcac
     4621 gttctgcacc ttggtgcccg gcaacgcatc ttcaacggtc ttcgcgaagt tctcgaacac
     4681 gatgatggtt tgcgcaccgc tgtccttcag ttgatgcgcc agctcgcgca ccgtgtagag
     4741 cggattcacg ttgaccacca cgccgcccgc cttcagcacg ccgaacagcg acacgggata
     4801 ctggaacgta ttcggcagca tgatcgcgac gcgctcgccg ggcttcacgc cgatgctttg
     4861 cagatacgcg gcaaatgcgg tcgctttacg gccgagttcg ccatacgtca gattcgcgcc
     4921 gacgctcaca tacgcgacgc gctcgcgaaa ctgcgcgatg cattcgtcga agaactgcac
     4981 gatggagtcg tactggttga cgtcgatttc gtgcggtacg tcggcgggat aggacgcgta
     5041 ccagatgccg tcggtcctgg gtgtctgagt gatagcccgc tcgggcgcct gaccaggcgt
     5101 ttgagcgggc gcctggcccg ctgcattcgc gggcggtgtg gatccggcgg cgggcgagag
     5161 cggggtttgc gtggtctggg tcatcggtca tctcctgaag ccgtggcttc gtatggttgt
     5221 gtgctggtct tgcaggcagt cagcgttcga gaatcgccac cacgccctgg ccgcccgccg
     5281 cgcagatcga gatcagcccg cgcgcggtgc cggccggttt gtcgagttgc gcgagcattt
     5341 tcgcgagtcc ggcgacgatg cgcccgcccg tggcggcaaa cggatggcca gtggcgagcg
     5401 agctgccgtt cacattcaat tttgcccgat cgatcgcgcc gagcggccca ttcattccga
     5461 gctgcgtgcg gcaatactcg tcatcctgcc acgccgcgag cgtgcacaac acttgcgcgg
     5521 cgaaggcctc gtgaatctcg tacagatcga agtcctgcaa cgtgagcccc gcgcgggcca
     5581 gcatacgcgg cacggcgtag gcgggcgcca tcagcaggcc ttctttcttg tcgaaaaaat
     5641 cgacggcggc ggtttcggac cagctcagat acgccagcac aggcaaatcg tgtctggcgg
     5701 cccaggcatc gctggcgagc aacacggcgg aagcgccatc cgtcagcggc gtcgaattgc
     5761 ccgcggtcag cgtgcccgcg tcgcgatcga ataccggctt caaaccggcg agtttttcga
     5821 gcgtcaggtc ggcgcgcaga ttgttgtcgc gcgcaaggcc acggtatggc gtcatcagat
     5881 cgttgacgaa gccgcgcgca tacgcctcgg tgagcttgcg gtgactgtcg taagccagca
     5941 cgtcctgcgc ttcgcgcgag atgttccagc gcttggccat cagttcgcaa tgctcgccca
     6001 ttgaaagccc tgtacgcggt tcgccgttgc gcggcaacag cggtttgaaa aacatgccgg
     6061 gccgcagttt ggtcagcgca ccgacacgtt ggccggtgct cttgccgcga tttgcttcga
     6121 gcaggatctt gcgcatgcgc tcgttgacgc cgatcggcgc atcggatgtc gtatcgacgc
     6181 cgcccgcgat gcccacttcg atctgcccga gcgcgatctt gttggcgacc agaatggccg
     6241 cttccaaccc ggtgccgcag gcctgctgta catcgtaggc gggcgtttcc cgggcgactg
     6301 tggtggacag cacggattcg cgcgtcaggt tgaaatcgcg cgaatgcttg atgacggcgc
     6361 cggccgccac ttcgcccagg cgttcgccat gcaggttgta gcggtcgatc aggccttgca
     6421 gcgtgaaagt cagcatgtcc tgattcgacg cggtcgcgta ggcggtgttc gagcgggcaa
     6481 acggaatccg gttgccgccg atgatggcga cgcggcgcac ggcgggcagc gggttggaca
     6541 tgctcggctc ctttgggtcg atccctggtg attgatctgc ttattgagcg gctcattgac
     6601 cgttcattga ccttatcaat gaacggtgcg aatggtgatc aatgtgtctt ttacgttcgg
     6661 cttcgcgttt gtcttcgttc tggctcaaca acgattggca gacggctccg attccaggcg
     6721 ataacgaaac cacgcgcgcg cctcacagca gcctccactg catgcgcccg cgcaaatgcg
     6781 gtttgtctcc ggtgatatcg cgtacctcga ggtcgcgctc gatcagcgaa ggcgacgcgc
     6841 tccacagcga cgcttcgccc ggcagcaaca tcggcagctt gaattcggcg gagagcgcgg
     6901 cctcggcgag cggtttcggc ggttgcagcg cggcggcggc acgcgccagc gtccacatgc
     6961 catgcgcaat ggcgcgcgga aaaccgaatg ccttcgcgga cagcgcggtg agatgaatcg
     7021 ggttgtagtc gccggacacg cgcgcgtaat cgcggcccag ttgcggcggc agctgccagc
     7081 gcgagatgcg ctgcagcgga tcgtgcccga gctccagcgg atcgagcgga ctgccgctcg
     7141 ccggcacgcc gcgtttcagg tagacgcttt cgccgtccca gaccgcttcg ccgcgccggt
     7201 acatgcgggt atgcagtacg aacgcctggc ccttgtcgtg ccgcagcagc gcgccgaact
     7261 ccacctcgac gcgcaacagg tcccggtagg cgagcggacg acgcagccgc acatggttcg
     7321 ccagatgcac gagccccaga gccggccacg gaaacgccgg gtcggtcagc atcagcagat
     7381 gcagcgggaa ggcgagcaga tgcggatacg tcagcggcac gccgtgctcg ggaatgaagc
     7441 cgcagacccg cgaataacgc gagatcggtc cggggtcgag cgccacggcg gggcgcgcca
     7501 ggcgcaacgc cggcaggtgc gtgtcgcgtc cgcgtttgac gatgccggac aacgcgcgcg
     7561 cgtacagctt cgccggcgcg ggcaactgct cgatgacgac ggtcttcgga cggcctgcgc
     7621 tcgctgcgtg accactgcgg aacggctcac ccatgctcac gctccaatca ggctttgtcc
     7681 gcacacgcgc accacctggc cgctcacgcc cgccgctccc ggatgggcga gccacgcgat
     7741 ggtctgagcg acatccaccg gctggccgcc ctggctcagc gaattcatcc ggcgcccggc
     7801 ttcgcggagg gtcagcggaa tcttcgcggt catctgcgtt tcgatgaagc cgggcgcgac
     7861 cgcgttgatg gtgatgccgc gtgcacgcag atgcggcgcc atgctttgca cccggccgat
     7921 cacaccggct ttcgaggtag cgtaattggt ctggccgaga ttgccggcca tgccgctgat
     7981 cgacgacacg ccgatgatgc ggccgccgtc gcgcaagatg ccggccgcga gcagggcgtc
     8041 gtcgatccgt tcctgggcgg acaggttgat gtcgatcacg ctttgccacg cggcatcggt
     8101 catcttcgcg atggttttgt ccctggtgat gcccgcgttg tgcacgacga tatccacacc
     8161 ttgttcgtcg agcgccgcgg cgatctgcgc gggcgcttcg ggcgtggcga tgtcgaaggc
     8221 gagggcgatg ccgttcaact ggcgcatcgt cgcatcgagc gcgtcgcggg ccgacgggat
     8281 gtcgatgccg atcacgtgcg cgccttcagc ggcgagcacg ctcgcaatcg aggcgccgat
     8341 gccgcgcgcc gcgccggtga ccacggcacg ccgggccgcc agcggctggt tccagtcgaa
     8401 cgccggcaag gcgtcggcgc cggcgcgcac gccgatgcgc accacctggc ccgacacata
     8461 cgcggagcgc ggcgatagaa aaaatcgcaa cgtcgcctca atgccgttct cggcgccttg
     8521 ttccacgtag accagattcg cggtaatgcc gcgtcgcgct tccttgccga gcgaacgcgt
     8581 gaggccttcg agcgcgcgct gcgcggtcca ttggcgcggc gtcgcgcagc tttccggcgg
     8641 acgccccagc acgatgatgc ggccgcactt gccgagcgag cgcaacgtgt catggaagaa
     8701 gccatgcagc ggttcgagct ggctgctgtc ctcgataccg ctcgcatcga acagcagcgc
     8761 ggtgagcttg ccgtgcgaac cggactccgc cggttcgaaa cggcctgtca tcagaccatg
     8821 gcgattcgca agcggcaccc acagcccggc gctttcgtgc gccacgctgg tcacgccgat
     8881 gctgttgacg agattcgcga gcgcgtcgag cagatgcggc tcgcggcccg cgccgatcgc
     8941 gatcaggccg tcgaactcgg gctggtcggc gcgatagcgg cgcagtacct cgggcttcgg
     9001 taaacctagc gagcgcgcga ggcgcgcgcc gaacggcgaa ttgacgaagt tcaggtaaga
     9061 gtcgttcata ttgttggttg ctccgctggc cacgtccgga cgatgtcctc cgccccgaag
     9121 gaagtcccgt tgggggattg ggggctcgga cgtgtgtcag tgtgcaccca gtttggggca
     9181 tctggaggtg ccccgcctgg atgttttttc aaggcgctca ggccgcgagc tccagcgctt
     9241 tttcgagcgc gtccttgcgc tgttgcaagt tggcgagcat gtcgaagtcg gccgggaagt
     9301 cgtcgacctt cacgacctcc gcgccatagc gcgcatagtc gtcgagcacg cgacggtcgt
     9361 cggtgtcgat caggcctttg tgctgcgccg cttcggtcca cgccgccaac tgcagcaggc
     9421 tctgaggcat gggttcgagc agcccttgtt tgaccgcttc gcgcagcttc tgctcgattt
     9481 ccgtgaagcg cgggttcagc tcgaacacga gctcgccgta ccccaacgca tcgacatccg
     9541 gatgcggcac atacgagtcg gacacgaggc gctcgcgcgc cgcgcccggc gtttgcatca
     9601 gctccgcgat ctcgctgccg aggcgatcgg acggctcgcg atacggcagg ccgaacggaa
     9661 atgcgagaac acgcacgaga cccgcggcga ggcggttcgg gtaattcgcg agcaccccgt
     9721 cgagcgcgtg ttgcgccttg tacagcgaat cttcgacgcc ccagcgcact agcggcaaat
     9781 cttcttcctg ccggccttcg tcttcgaaac gcttgagtgt cgcggagatc aggtagagct
     9841 gcgagagcac gtcgcccaga cgcccggaaa tgcgttcacg ccgtttcaga tcgccgccga
     9901 gtacgaacat ggagacgtcg gcgagcaacg cgaatgcagt cgagaggcgg gtcgccgcgc
     9961 tgtagtacga cagcagcggc gtgtaggcca tgcgcggcct ggcgatgaac gcgccgcccg
    10021 tgatgccgta aacgaaactg cgcaccgcgt tggagagcgt gaagctgacg tgaccgaaaa
    10081 aggccgcatc gaaatcgcgc agcgcgcggg cgcgatccgt ttcgcgcgtg gccgccattt
    10141 ccttcagcac atacggatga cagcgaatcg caccctgacc gaagatgatc aggcaacgcg
    10201 tgagaatgtt cgcgccttcc acggtgatcg cgatgggcac ctgctgatag gcgcgcgcga
    10261 gaaagttcga cggccccatg caaatgccct tgccggcggc gatgtccatg ccgtcgttga
    10321 tgaccatgcg ggcgcgttcg gtgatgtgat acttcgcgat cgccgaaatc accgacggtt
    10381 tctcgccgag gtccaccgca tgcgcggaca aacggcgcgc ggcatccatc acgtagagat
    10441 tgccgcccat gcggccgagc gcttcctgca cgccctcgaa tttgccgacg gcggtgcgga
    10501 actgccggcg aatcgcggca taggcgccgg tgccgcgcac ggcgatcttc gccatcccca
    10561 cattcgacga cggcagcgaa atcgcccggc cggcagcgag acattccatc agcatgcgcc
    10621 agccgttgcc gacttgcgcg cggccgccga tcacccagtc gagcggaatg aagacgtctt
    10681 tgcccgagtt cgggccgttc tggaataccg cgttcagcgg ccagtgacgg cgcccgatgt
    10741 tgacgcccgg atggtcggtc ggaatcagcg cgcaggtgat gccgggttcg tcattcgagc
    10801 cgagtagatg gtccggatcg agggcgcgga acgccagacc gagcacggtc gcgatcggtc
    10861 caagcgtgat gtagcgcttg tcccatgtga cacgaaagcc gagcgtctcg cggccttcga
    10921 acgcgccctt gcagacaatg ccgaggtcgg gaatcgccgc cgcgtccgaa ccggcgtacg
    10981 gactcgtcaa tgcgaagcag gggatctcat cgccacgcgc gaggcgcggc agatagtggt
    11041 tcttctgctc ttcggtgccg tagtgcatca gcaactcggc cggaccgagc gagttcggca
    11101 ccatcaccga gacggcggcg gccgagcagc gcgtggcgag cttcatgatg acctgcgaat
    11161 gcgcgtaggc ggagaactgc ttgccgccgt actgcttcgg aatgatcatg ccgagaaagc
    11221 cgcgttcctt tatgtactgc caggtgttcg gcgacaggtc ttgccacacc gtggtggttt
    11281 cccaatcgtt cgcgagatcg cacaggtgct cgcattcgac atcgagaaac gactgttctt
    11341 ccgcggtgag cgtggccggt ccgtagccga gcagcgtgtc ccaatgcggg cgcccggaga
    11401 acagttgggc gtcccaccag acggtgccgg cttcgatcgc gtcgcgctcg gtcgccgaca
    11461 tctccggcag gatcgtgcgg aaaatgtcga gcaccggctt cgtgagccac gcgcggcgca
    11521 gcggcttgat cgtgagaacg agggcgggga ggacgaagac gatcgcgagt aacgttgtcg
    11581 ccaccgcacc cgctgctccg ctgagatgcg cggccgctac ccagacgatc atgaagccaa
    11641 accaccatga ggcgcgcgcc tgcacataga cgagcgccag agcggcaatg acgagcacca
    11701 ggatgaagca cggcatgacg tttcctcctg tttcgatggt gcatgcgaac gcatccacgg
    11761 gtcgcggacg gctcgcgttc tgtcgatctt ctactggcag tgttgctgta cgccgcgtgc
    11821 tcggtgcgct ctcaaggcgc cttttggccg cgctgttcag tcgttttttt cagtggctct
    11881 tgagtggttt tcgagtcaca cctctttcgc tgctcgtgtc gtgtctgcgg ttcgactgcg
    11941 tgacgttgtc caggtgcggg acttggacgt gttgctcagg caggcggtat tcacgatgcc
    12001 tgaacgcgga cggcctgtag taccgccgcc cgcgccggct cctgttcagg ccgtgccgtg
    12061 caacgcgggc tgaaacgcac acgcctccat gttgccaccg cggcgttcac cgtccgagac
    12121 tgccggcgac gacgattcaa acccgcctga tgcctgctgc gtacccgcag cagggttcat
    12181 gccttccacc tgagattccg gtccgctctc ggtggcgtcg cccagcacgg ccgcgaacgt
    12241 ggccagttgc gagccgtcgg gcagcggcgc ctttagtgcg gccaccatca atgaagcgag
    12301 acgtgcggtc agttgcaggt cgttcatcga cttgccctgc gagaactcgg aaatcaggct
    12361 gtcggtatcg gtgccggcca acaccccgga cagtgcgccg ttcgcgaagt gcagacgcca
    12421 gccgagttct tcacgcggca gatgcggcag cgcacgctgg aacgcatcga aaaagcgcac
    12481 agccaccgac gcgtaatgcg cattcagaaa gtcccgaatg aacgccgacg gatccgtata
    12541 agcgcggccc agcagacgca gaaacgcctt gccgccgacg cgcggttcgc gcgacagtcg
    12601 cagcgcggga atgaacatgg cgccgagcac gtgctcgcag gtcagacgcg cgccgagcat
    12661 gtggtcgaag cggtcgagca gcttgagccg ctcctggttg agccggtcga gtcggcgcga
    12721 cagcatcgaa tgaatcagcg attccttgct gccgaagtga tagttgaccg ccgcgagatt
    12781 cacttcggcg cgcgaagtaa tctggcgcag agacatggcc tcatagccgc agtcgatgaa
    12841 gagagtttca gcggcgtcga ggatgcggga cttcgtgtcg ccggcatgcc ggcctgttgt
    12901 gcgaactgcc attttgcgtc tcccagttgc cgggcatccg gcccgcaaac atgcgattca
    12961 aatgactatt tgaaacagcc gtttttttca ggataaggaa ggatcaggac cgcgatcaag
    13021 ggttcgatgt ggactaactc ctctagaaga cctgacgaag gtccgtcttt ccaatgcgcg
    13081 cgggcgcgtc aaaccgcgcg gcaataaaaa aggccgttcc gattggaatc ggaacggcct
    13141 cgtattgcca gttacctgtt ttggccctgc gtttgcggtg tttccgcgtt gttttgcggc
    13201 ggttcgagcc gcctttgtcg ctattgcgcc cgataactcg ctggcgaaat agccgtttag
    13261 ttcgaacgct taaagctgtg ccgcaacctg tttcgccgac tgcgtcatgt cgatgccgcg
    13321 gcgttcgcgg ctaaacggaa tcagcgcggc gatcaccacc gccacgatac cgatcacgac
    13381 ggccatgatc agcgcgtagt tgttgccgtg tgcttcggcg gcgctggctt gcagcgggcc
    13441 gttcaccgac gcgatcagat tgccgagctg atacacgagg cccgggaacg tcgcgcgaat
    13501 ttcatcgggc gagatttcgt tcaggtgcac cgggatcaca ccccatgcgc cttgcacgga
    13561 gatctgcatc aggaacgcgc cgacggcgag tagcaccggc gtggacgaaa aagcccacag
    13621 cggcagcacc ggcaatgcga tcaaggcggc gatgaagatc gcgcgcttgc ggccaatcct
    13681 ctccgacatg tagccgaaga acagaccgcc gcagatcgcg ccgatgttca gcgtgatcgt
    13741 gatcaacgac accgtatgcg catcgaaccc atgctgcacg cgcaggaagg tcggatacag
    13801 atcctgcgaa ccgtgcgaga aaaagttgaa cgcggtcatc agcacgatcg agtagagcga
    13861 taacccgacg ttctgcttca gcgtggcgat gagacccgga cgcgctttct tttcgagcgt
    13921 cttgaaggct ggcgactccg gcacgtgcgc acgcacatac agcaccagca acgccggcag
    13981 cacaccgacg aagaacatgc cacgccagcc gatgtattga tagaagacgc cgaacacgat
    14041 cgaggcgagc agatagccac tcggataacc agcctgcaga agacccgaga cgatgccgcg
    14101 cgatttcggc ggtacggttt ccatcgtcag tgcgccgcct acgccccatt cgccgcccat
    14161 ggcgatgccg aacagggcgc gcagcacgag cagggtggcg aggttgggcg agaagccgga
    14221 cagcagttcc agcagcgaga agcacgcgat gttgaccatc agcgtgggac gacggccgta
    14281 tttgtcggca agccagccga aaatcaatgc gccgaccgga cgcatcacca gggtcagcat
    14341 aatgccgaag gcaacctcgg gaatttttgt attgaactcc gccgcgatat ctttcaatac
    14401 aaacaccatt agaaagaaat cgaatgcatc caaggtccag cctagatagg cggcgatcgt
    14461 gacgtttctc tgttcccgtg tccagctcat tgattgttct cctgagtctt caataacccg
    14521 tgcttaacga cgggcggccc tgcggccctg cagccctgta cgccccgtgg tacggacttc
    14581 gagcgagtgt acgccgagcg ttaagcgctt gcatgaatga acccacgttt atccgtatta
    14641 ataccaggaa cgaattccgg tgaaaatctc aatgcatggt aaacgtcggc ataatgtaat
    14701 tatcgtatta atggtgtttg atttattgtt ggttgtctgt aatgtcaatg gcatgtctgc
    14761 ggatggaaat ccggtcaatg acttgcgttg tatgggtatc ggtaaaggcg acactcgcgc
    14821 gattcgcggg atgcgttcag gtataacagc ggctcgccag ggaggtcgcg tcgttcatat
    14881 atgcgtacac cgcattagtc gagcataaaa atcaattgga ttcatgttgc ggcgcggaat
    14941 aaaatggcgg ctgtcttatt aatgtcgctt gcatttccgc accatgtcat tcaaatcaat
    15001 cacttcggag cctgttcgcc acgagcagcg tccattggcc gtgctggccg cagtcacttt
    15061 gctgaccggc atcggcgccg gtctcggcgg tatgttgctc gccttgctgc tgcactgtat
    15121 tcagcatctc gcatacggct acagcgtgac gcatgtcatc agcagcgaga gttttctgca
    15181 aggtgtcacc gatgccgagc ctgagcggcg tgtgtttgtt ctgacgattt gcggactggt
    15241 ggccggactc ggctggtggt tgctgtatcg cttcgggcgg cccctcgtga gtattcgcca
    15301 ggccgtcaaa tcggacgatc cgcgcatgcc cttgctgagc acgaccgtgc atgcgttgct
    15361 gcagatcgtg acggtcgcgc tgggctcgcc gctcgggcgc gaagtggcgc cgcgcgagat
    15421 cggctcgatg ctggccggat ggttgtcgca tcgcgcgggg ttatcggtcg agcagaaccg
    15481 gatcatggtt gcgtgcggtg cgggcgcggg gctggccgcc gtctacaacg tgccgctcgg
    15541 cggcgcggtg ttcgtgctcg aggttctgtt gggtacgttt ggctggaagg cagcggtgcc
    15601 ggccgtggtg acatccgcgg tggcggcgct ggtcgcgcag gccggtctgg gcaacgagca
    15661 tcagtatctg gtgccgccgt tgacggtgga cacctcgctg atggtgtggg ccgtggtgtg
    15721 cgggccgatc ttcggtattg cggcgtgggg ttttacggag atcacgaagc gcgcgcgtgc
    15781 ggatgcgccg aaagattggc gtttgccggt gttgtcgctg ttgaacttcg cgatcatcgg
    15841 cgtgctggcg atgcattttc cgcagttgct cggcaatggc aaaggacctg cggcactggc
    15901 gttcgacggc gacctgacgg tgagtctcgc ggcgatgttg ctggtgctga aggcggtgat
    15961 taccgcgggt agtctgcgcg cgggtgctga aggcggtcta ttgacaccag gactggccaa
    16021 tggcgcgttg ctggcgatcg tacttggcgg ggcctggagt ctgttttggc ccggggcttc
    16081 gttcggcgct tttgctgttg tcggcgcgac ggcgtttctg gctgcatcga tgcagatgcc
    16141 gattacggct gttgttctcg ttttcgaatt tacgcgggtg agccacgatt ttctgatccc
    16201 ggttctgttc gccgtcggcg gtgccgtgtt gagttttcaa ggatgcgtca agtgggcgcc
    16261 ggcgctgcag tttggttctg gcttcaattg gagtggcgcg ggcaaggctc gataaatttt
    16321 tgtcctggcg gcgaccttcg ctgttcgagc cggtgggcgg gtgatcacgg ctcggcgaac
    16381 gccgctattt caagcctcgc tgcttgcgat attcgtcaac cggcagaccg ccccagcccc
    16441 agttctccgt ttccacctcc tggatgacca caaaggttgc ttcgagtggc ttgttcagca
    16501 cgtcgagcag caggtggctg acgcccctga tgagttcagc cttttcttcc gcggtgaccg
    16561 agtcgacgcc tggcctcgtg ccctcgcggg ttacctggat cgtgacgatt ggcatggtgt
    16621 tctcctctat cgtgattgac gaaccagccg ccggctagca gcggcgtccg gtcgtgtggg
    16681 acgatgcctg gcattgcagg tgtttgccgg tcgcgcttag tgcccagctt agtgcccggc
    16741 gctctggccg ccgtcgacat gcaggatctc gccggtgacg aagggggcgg aatccagata
    16801 cacgatcgcg ttgacgatat cgctcatctc gcccatatga cccagcggat ggagcgcgcc
    16861 gagtgccgca tgcgtttcga ccgcgtgcat cggcgacttg atgatgcccg gcgagacggc
    16921 gttggcacga atgccgcgct tggcgtattc gatggcgagg gaccgggttg ccgcgttcag
    16981 gccgcccttg gtgagcgagg cgagtaccga aggcacgccg tcgatcgcgt gctcggccag
    17041 cgtggtgctg acgctcacga cgtggccact ggaatgcttc tccatttccg cgatggcaag
    17101 ctgcgtgatg tagaagaaac cattcaggtt cacgcccgtt atcgtcgcat agtcttcagc
    17161 tgtgtagcgg gtaaagggct tggcgatgaa gacgccggcg ttgttgacga gcgtatcgat
    17221 ccggccaaag cgcttcacgg cttcgctgat cacgcggcgg gcggttgcgg ggtcggcgat
    17281 gtcgccggcc acggtcagga tgttgggatc ctccgacggc ttgatgctgc gcgcggtggc
    17341 gaccacgcga tagtcgagct tgcggaatgc cttgacgact tctgcgccga tgccttgcga
    17401 tgcgccggta acgacgacga cctttggtga cgtgctcata gtgaagctcc gaatcaaatt
    17461 gagtggactc tgatgccgat cgggctcatg gctccgatag ggagctaatc taggcgcttt
    17521 tgcgtcgagt tcgaataccc ggcgtggaca aacacttgtg cttcgacgga acaaatcaga
    17581 cgcgacgggc tcggacgcat actttcccgg gggatgcgcg agtgcgccgc gccaggtgga
    17641 tgacctgtcc ggttgcttta ctttgccgtg taccgatgcg catccggcgt agctgcactt
    17701 ccttgaagtt cgcatcgacc cagacgccgt cgacgtgtcc accaaaaatt gacgataatg
    17761 cccccaagga acgattacgg gtcgtgctcc gtcgtcggag acgccgctga atctacaaga
    17821 aaccatgaga cgactgctcc tcgctgcgtt cgcagccacc tttttcgtga cttgcgttca
    17881 tgcgcaaagc aacgcttttg gcccgctcgt cacgccctcg ggcaacctgc aattcgtgcg
    17941 tgtcgaccgc gatttcgtgg ggatgctcga caaggagatt ttcgatcgct tcggcgcgag
    18001 cacgctcacg catttcgatg acgtcggcaa cgcaggtgac accgtcacac gcatgctcgt
    18061 gcagacggat gccggattgg tgctgtacga ctttcgccac cgtccgccac ttgtgcagcg
    18121 ctcggataag cgcatgatgg tcaagcgcgt gttctggcaa gacgatgaag tcgtgatgca
    18181 aagctcgcaa ggctggttcc gattcaaacg cggcgtactg acgaagctgc agtcgtctac
    18241 gacaacgtac cattgattac acgcgcaccc cgaacacata gtcgcgagcg atgacatgca
    18301 ttgatccggt cggccgcgat ctggactggc ctgccggaac agcgcaccac ctccgatagg
    18361 gctagggtcg atgtgatcga cgggagtggc cggtgtcgct ctgcggtatc ctgcttgcat
    18421 cgatcccgat cacctggacg tttcccgaaa ccgaggagtt gcgatacatg aacgagcctt
    18481 caccaagcat cgaaatcgaa actgcgccga atccagggtt cgccgtcatc ctgatgcacg
    18541 gtctcggcgc cgacgccaat gacttcgtgc cgcttgtccc cgaactacgt ctcacggatg
    18601 cgcccgctgt gcgcttcgtg tttcccaatg cgccggagat tgcggttacc gcaaacgatg
    18661 gctacgtgat gcgcgcgtgg tacgacatcc ggtcgtttca ggacgttaat cggcaagtcg
    18721 acgaagccgg tatcgaatcg tcttgcgcga ccgtgcggcg attgatcgaa gcgcagaacc
    18781 agcgcggcat tccgacttcg aacatctttc tcgcgggttt ttcgcaaggc gcggcgatga
    18841 cctattcagc cggattgacg caccctgagg cgcttgccgg cctgatcgtg atgtcgggtt
    18901 atgtgccgtc gcgaggcttt gtggacagcc ggttgagcgc tgcaaacagc agtacgccga
    18961 tttttgccgc gcatggccta tacgacgacg tgttgccgat ccagttaggg gaggccgcgc
    19021 gtgactttgc gatcgcgaaa ggttgcaacg tcgactggaa cgcgtatccg atgccgcatt
    19081 cggtgtgtga ggaggagatc tcggcgttgc gcgtgtggct cggcgcgctc ctcacggcct
    19141 cttcctgaat cgttccgcag cgctcacgaa ttgattgccg tgtcctgcca gggatgggtg
    19201 ttcttgtaat ggaacagtgg aacaagacgg cccatgccgt tcgtgatcca tagctgcatc
    19261 atcacgcggc agaacgagag aagaattagc gtgcggcatg cttcgcaagc gatgtgcttt
    19321 caggttattt tccgatgaaa ttgagtgcat ccagcatcgg cgtcttcgac acattgacca
    19381 ttccctgaaa tggcgtgtcg agatcgagga caacaaacac ggcagtcgtg gccgatgcgg
    19441 cgcaaagaaa aaatacgacg cacatgctcc agtttctggg cgcactaaaa ccgaatatgc
    19501 cgaatatgac agccagccag ccaacgagga ccacggttag aggagcggga atcaagccct
    19561 ttctgtgttc gaatgcgagc catcgaccgg caaatatctc gtcccaaagt tccatcgcgc
    19621 ggtcacggag tctttcctgc gaagcaccga ggggctggaa agcgaaaatg cgcccctgga
    19681 tgtcgtaagt actctggagg atttctcgcg tttctgcctc tgcggtcgtt cccgtcttgt
    19741 tggaaaacag gaaggagatc caccgggcgt agtcgttctt tatctgaagg cgcaggccgt
    19801 cggccgatgg gccaaactgg gacaaggttc ggtccagccg gatcatcgtg acagcattat
    19861 gttggagcgc agtgttgacc aggtcgagcg aggactttcc ggacgaaatc agcaggctaa
    19921 gcaccaatgc cgacagcgtc gccacgagac caacggcaat acgcatcgcg gcaagcgagg
    19981 cttcctcgaa atggtgatct gccagtcttg agcgcacata cgacccaagc gctgcgctcg
    20041 aaagaaggag caccagcacg aaggcagaaa agacatagtg atccatgtgg aaatgacctc
    20101 gttcgatcgc acgggttggg tgaaccttgt cgagcaggcg gcagtcatgc tgcagattat
    20161 atggctggcg gcgcaagagg ctgtgcgtta cgtgcggctc gatcaataag tcgcacgacc
    20221 gcctgaagcg tcgaacacgg cacctgtcga acacgagcat tcttccgaac acaaccaacg
    20281 cacgcgtgcg gcgatttctt ctactaaacc gggccatccc atcgggatcc ggccttgcag
    20341 cgcaccgagc tggtccacgg gcatttgagc gcggctagcg cgaaaggcat cgcccctgtg
    20401 atgccatcgc cagcgcgttc ctgttggttg ggttgctcgt tttcgggacg tcgcctggct
    20461 gtattgcacc gtaagtaaag cttatcagcg ggaggtggca tgtcgttcca gattggaatt
    20521 ttatttcata ttacgaaagc atctcgcctc gtcgatcgtc ttctgatgag caaaattcat
    20581 tggagactga catgcccggc gtcaccgctc gcgatcgtcg agcgtctggc cacgtagatg
    20641 gaggaacacc gttggcgatg atcgtgagcg agctcgacat ttcgcacagt gcgtgtcagg
    20701 aggctacttg tcgatctcag gcagtgcggg tacgtccgtc agttctgtcg aacgtcttca
    20761 gtgcgtagag gagggcaatc gatgtgaatg aatagccggc gccgttggct tggggcggct
    20821 tcgcccacga ttgcattggc ggcttgaatg ctactcacgc cgagccacgc cgcaaattcg
    20881 agcgcgcacg aaatgtcgaa ttgcgcagcc ggctcgcaac tgcacgaatt cgtcgacagc
    20941 tcgcgaatgt tccgtcatgc cgatcagcgt cagcgcattg tgtgcgccag gacgcatggc
    21001 ggtctcaaac acaggcttga gcagcagggg aagcggaggg attcgttttg cgattgctga
    21061 cgcattctcg cgatggcgat ctgaccatct cggcgatgcg catgcatcct tgaaactgat
    21121 tacgggtggt ttgggaatcg gtcccggtct cgtgcagaac gtcgagcggg aaggtttgct
    21181 tgaggaaagt cgcagcgcca gtgcggcagt cgaagaccgc gccgtcgtgc tggccgacgg
    21241 cggggcgccg tggtccaggc actagcaggc cgatgacccg ccggccgcga cgacgcttca
    21301 acaaaaaacc ccgtaagacg ttcatcttac ggggtttctc gaactacatc ggcttacgcc
    21361 ggaacaactg atggtgccca ggagaggact cgaacctcca cggtgttgcc accgctagta
    21421 cctgaaccta gtgcgtctac caattccgcc acctgggcac gtcttcaagc tagaccgcta
    21481 ttttagcgag ctgatgcagc gtgtcaatcc cttcatgcaa acaaactatc gcttcatcaa
    21541 gcgccgggtt ggtgaagcgc agcgaggcaa aacctcactg ctcaatcact gccgactcac
    21601 gtcgacgcaa cgtccgctaa tcacgccatc cgatccggaa aaacaaaccc ggatcacatg
    21661 gcttcaccca aacctcgcac aaaaaagtaa agccgcccgg aggcggcttt acttttgaat
    21721 ctggtgccca agagaggact cgaacctcca cagtgttgcc accgctagga cctgaaccta
    21781 gtgcgtctac caatttcgcc acctgggcag g
//
LOCUS       DATASH010000739         5897 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6647991,
            whole genome shotgun sequence.
ACCESSION   DATASH010000739 DATASH010000000
VERSION     DATASH010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5897
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6647991"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VHV99_19705"
     CDS             <1..215
                     /locus_tag="VHV99_19705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HEX3637666.1"
                     /translation="LTGTLPTLSREEAKEMLEAAGAKVAGSVSKKTDYVVAWAEAGSK
                     LVKAEELGVPVLDEDGMRKLLEGETP"
     gene            212..745
                     /gene="def"
                     /locus_tag="VHV99_19710"
     CDS             212..745
                     /gene="def"
                     /locus_tag="VHV99_19710"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX3637667.1"
                     /translation="MIREILKMGDPRLLRIAESVDHFDTPELHALVEDMFDTMRHANG
                     AGLAAPQIGVDLQVVIFGFAQNERYPEAEPVPETVLINPIITPVSHDMEEGWEGCLSV
                     PGLRGAVQRYTMIRYQGFDQYGSPIERLAEGFHARVVQHECDHLIGKLYPMRVTDFSK
                     FGFTEVLFPGLDPKSDD"
     gene            complement(989..1228)
                     /locus_tag="VHV99_19715"
     CDS             complement(989..1228)
                     /locus_tag="VHV99_19715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637668.1"
                     /translation="MSVEIIVRETKTGLEIVSGRRRLDALLAVRDEVVVTSPGVGEVV
                     IARLPDGRLQASANPEHIALFRQPELASGPKKSFA"
     gene            complement(1446..2195)
                     /locus_tag="VHV99_19720"
     CDS             complement(1446..2195)
                     /locus_tag="VHV99_19720"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX3637669.1"
                     /translation="MRLSKVMSELGLCSRREADEWIEKGWVLVDGEVIDTLGTKIRPD
                     QNVEIAPAAHAAQMQQVTILIHKPVGYVSGQAEDGYQPAVTLVNPSNHWADDQSGIRF
                     TPLHLRTLAPAGRLDIDSTGLLVLTQDGRVAKQLIGGHSDVDKEYLVRVKFGDLEVDI
                     EPHFPPESLALLRHGLSLDDVPLKTAQVSWQNGEQLRFVLREGKKRQIRRMCELVGLE
                     VVGLKRIRMGRVTLGALPQGQWRYLGADEAF"
     gene            complement(2333..3145)
                     /locus_tag="VHV99_19725"
     CDS             complement(2333..3145)
                     /locus_tag="VHV99_19725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637670.1"
                     /translation="MVTVRLAMVNVVRMRLAATLRVRLVVIVGPGLPLGTVVLRVVIG
                     RLVMVNVGRMRRVVTLRVRLVVIVGPGPRLGTVVRPVAIGLLAMVNAVRTRRVGMLRV
                     VRTATAVRVLRLVIVVLRVVIVRLAMASVVHMHHAATLRVVQAAIVDRVLRLATAAPH
                     AVTVRQVMVNAVRSRRAAMLHVVQAAIAVRAHPSATAAPHAQTARQAMPNAVRSNPAM
                     IRAARAAIARPAHHSMTAAVTVARPASAITRTMAARALLPPSANGPQATALT"
     gene            complement(3417..5888)
                     /locus_tag="VHV99_19730"
     CDS             complement(3417..5888)
                     /locus_tag="VHV99_19730"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006999385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEX3637671.1"
                     /translation="MHALARATDDALRGAWEACEMPKSAALIAVGGYGRGELAPYSDV
                     DILVLLPDEETLDHSVTLAARIERLIGMAWDLGLEIGSSVRSVSQCIDEASNDITIQT
                     SLLEARRIVGSEKLFETFEGRYRQTLDPRAFFQAKVLEMRQRHAKYQDTPYSLEPNIK
                     ESPGGLRDLQLILWISRAAGFGSSWRELDSRGLITEREASELRRNEGFLKTLRARLHV
                     IARRRQDILVFDLQTALAESLGYKATSSKRASEQLMRRYYWAAKAVTQLATILIQNVD
                     AQLFPSTSGITRVLNERFVEKQGMLEIASDDVFEREPNAILEAFLLYEQTRGVKGLSA
                     RTLRAIYNARDLMGRDWRRDPENRRLFMEILKQPDGITHALRLMNQTSVLGRYLLNFR
                     RIVGQMQHDLYHVYTVDQHILMVLRNMRRFAMAEHAHEYPFCSQLMGNFERPWVLYIA
                     ALFHDIAKGRGGDHSVLGTVDARRFCREHDVGADDSDLVVWLVEQHLTMSQVAQKQDT
                     SDPEVIKQFASMVKTERRLTALYLLTVADIRGTSPKVWNAWKGKLLEDLYRATRAVLG
                     GAELHAHSELKSRQEDALALLRLETVPENAEKVLWEKLDVGYFLRHDAADIAWQTRVL
                     YKHVESEKPIVRARPSPIGAALQVLVYVQDRPDLFAGICAYFDKNGLSVLDARVTTTR
                     HGYALDNFLVAHPDHDGHYRDIANLVEQQLSMLLTAEGILPEPSKGRLSRLVRTFPIT
                     PRVDLRPDERGQYYILSVSANDRQGLLYSIARVLAQHRIGVHAARINTLGERVEDVFL
                     LDGKTLSDNRTQIQVETELLRAIAA"
BASE COUNT         1093 a         1826 c         1858 g         1120 t
ORIGIN      
        1 tgctgactgg cacgttgccc acgctgtctc gcgaggaggc gaaggaaatg ctggaagccg
       61 cgggcgctaa agtggcggga tcggtgtcga agaagacgga ttacgtggtg gcgtgggcgg
      121 aggcgggcag caagcttgtg aaagctgagg aactcggcgt cccggtactc gacgaagatg
      181 gtatgcgcaa gcttttggag ggagaaaccc catgattcgc gaaatcctca aaatgggcga
      241 tccgcgtttg ttgcgtatcg ccgagtcggt cgaccatttc gatactccgg agctgcatgc
      301 tctggtggaa gacatgttcg acaccatgcg ccacgccaac ggtgcagggc tggccgcgcc
      361 gcagatcggg gtggatttgc aggtcgtgat cttcggtttt gcgcaaaacg agcgttatcc
      421 ggaggccgaa ccggtaccgg aaacggtgct gatcaacccg atcatcacgc cggtttcgca
      481 cgacatggaa gaaggctggg aagggtgttt gtcggtgccg ggcctgcggg gcgcggtgca
      541 gcggtacacc atgatccgtt atcaggggtt cgatcagtac ggctcaccga tcgaacggct
      601 ggcagaaggg tttcatgcgc gcgttgtgca gcacgaatgc gatcacctga tcggcaagct
      661 ttatcccatg cgtgttacgg acttctcgaa atttggcttc accgaggtcc tgtttccagg
      721 gcttgatccg aaaagcgacg attgattgat cgaggtgccc ttaaccgggt gccggctggt
      781 ttgacaggct gtccaaccat acgacgaata agggccgtga aaaatcgcgg cccttttgtt
      841 tagcgagatg cagcttttgt tgcagctctg gtatggcgca ggattagttg gacaatgcgc
      901 ccagccatgc cggacagaat ccggtcgttc agccggatga ttgacgcatc agcaaatcaa
      961 ggccgccgga gcccggcagc tttcatcgtc aggcgaagct tttcttgggt cccgacgcca
     1021 gttcgggttg gcgaaacagt gcaatgtgct ccggattcgc cgatgcctgc aagcgtccgt
     1081 ccggcaaccg cgcgatgaca acttcaccca cgcccgggct cgtcacgacc acttcgtcgc
     1141 gaacggcgag cagcgcatcg agtctacgtc ggccgctgac aatttcgagg ccggttttgg
     1201 tttcgcgaac aatgatctca acggacatgg caattctcca gtagcactgc gcccagcaca
     1261 cgcggctcga agcggtaaat tgcgcgtgta tctgactaac ggtcatgcgt catattttct
     1321 ttagccaaaa agttggccaa aaaaaatcga tgcgatcggc gggtgccgtt cgcatcgatt
     1381 ggtaacaatt tcgtcgtttt tccgcgacgc tttgaggcga gcggaaagcg tgagcaacgc
     1441 tgcttttaga acgcttcgtc cgcgcccaga taacgccatt ggccctgcgg caaggcgccg
     1501 agcgtcacgc gtcccatcct gatccgcttc aggccaacca cttccagccc gaccagctcg
     1561 cacatccggc gaatctggcg ctttttgcct tcacgcaaca caaagcgcaa ctgttctcca
     1621 ttttgccaac tgacctgcgc tgttttgagc ggcacgtcgt cgagcgagag gccgtggcgc
     1681 agcagcgcga ggctttcggg cgggaaatgc ggttcgatat cgacttcgag atcgccgaat
     1741 ttcacgcgca cgaggtattc cttatcgacg tccgaatggc caccgatcag ttgctttgcc
     1801 acccggccat cctgggtcag gacgagcagg ccggtcgaat cgatatccag acgtcctgcc
     1861 ggtgcaagcg tgcgcagatg cagcggcgtg aagcggatgc cggactggtc gtcagcccaa
     1921 tggttggacg gattgaccag cgtaacagcc ggctgataac cgtcttccgc ctgacccgac
     1981 acgtagccaa ccggcttgtg tatcaggatg gtgacctgct gcatctgggc cgcgtgcgcg
     2041 gcgggcgcaa tttcaacgtt ctggtcggga cgaatcttgg tcccgagcgt gtcgataact
     2101 tctccgtcga ccagaaccca gcccttttca atccattcat cggcttcgcg gcgtgaacac
     2161 aggccgagct ccgacatcac cttcgacaag cgcacgagcc cttcttcatt ttcgcgcgcg
     2221 atgcgggcgg gtgagggagg agcgtcggcg tcacgcggtt ttgacggctt cgataccggt
     2281 ttgcgcgcgg caggcttccg gtcgtcgccg aagcgtccgg atgggtcgcg tgctatgtaa
     2341 gggctgtcgc ctgagggccg ttggcgctcg gcggaagcag ggcgcgggct gccatcgtgc
     2401 gcgtgattgc gctcgcgggg cgcgccacgg tcaccgccgc ggtcattgaa tgatgggcgg
     2461 ggcgtgcgat cgctgcccgg gccgcgcgaa tcatcgcggg gtttgaacgg acggcgttcg
     2521 gcatcgcctg acgggcggtc tgcgcgtggg gcgccgcggt cgccgaagga tgggcgcgga
     2581 ccgcgatcgc tgcttggacc acgtggagca tcgccgcgcg gcgtgaacgg acggcgttca
     2641 ccatcacctg acggacggtc actgcgtggg gcgccgcggt cgccaaacga aggacgcggt
     2701 ccacgatcgc cgcttggacc acgcggagcg tcgccgcgtg gtgcatatgg acgacgctcg
     2761 ccatcgccag acggacgatc accacgcgga gcaccacgat caccaaacga aggacgcgga
     2821 ccgcggtcgc tgttcggacc acgcggagca tccccacgcg gcgcgtacgg acggcgttca
     2881 ccatcgccag aaggccgatc gccacggggc gcaccacggt ccccaaacga gggccggggc
     2941 ccacgatcac caccaggcgc acgcggagcg tcaccacgcg gcgcatacgg ccgacgttca
     3001 ccatcaccag acggccgatc accacgcgga gcaccacggt ccccaaagga aggccggggc
     3061 ccacgatcac cactaggcgc acgcggagcg tcgccgcgag gcgcatacgg acgacgttca
     3121 ccatcgccag acggacggtc accacggggc gcaccacggt ccccaaacga cggccgcgct
     3181 ccgcgatcgc ccgcgccacg cgagtcgtcg ctacgcgcct tgaaaggttt ccgctcaccc
     3241 gcacccgatg gccgtgcacc acctgccggt ttatcgctac gcgctggccg ctcgccgctc
     3301 gccatctcgg cgcgtggggg ctttttccga ccggcggcgc tgccggtacg gacgggggaa
     3361 cgtgccggcg atgccgggcg cgggtgcttc gctgtcagtt tgacgcgcat atattctcag
     3421 gctgcgatcg cgcgcagcag ttcggtttcg acctggatct gcgtccggtt gtcggatagg
     3481 gttttgccgt cgagcaggaa cacgtcttcc acgcgttcgc caagcgtatt gatccgcgcc
     3541 gcgtggacgc cgatccggtg ctgggccaga acccgcgcga tcgaataaag gaggccctgc
     3601 cggtcgttag ccgatacgga caggatgtaa tactgaccac gctcgtcggg acgtaagtcc
     3661 acgcgcggcg tgatcggaaa agtgcgtaca agccgtgaaa gccgtccctt cgacggttcc
     3721 ggcaagatgc cctcggcggt cagcaacata ctcagctgct gctcgacgag gttcgcaatg
     3781 tcgcgataat gcccgtcatg gtccggatgt gcgaccagaa aattgtcgag cgcgtagccg
     3841 tgccgtgtgg tcgtgacgcg tgcatcgagc acggacaacc cgtttttgtc gaaataagca
     3901 caaataccgg cgaacaggtc cggccggtcc tgcacataca ccagcacctg gagcgcggcg
     3961 ccaattggag acggccgcgc ccgcacaatg ggcttttcgc tctccacatg cttataaagc
     4021 acgcgggtct gccaggcgat atcggcggcg tcgtggcgca ggaaatagcc gacatcgagc
     4081 ttctcccaca gcactttttc cgcgttttca ggcacggttt caagccgcag caacgcgagt
     4141 gcgtcttcct gacgcgactt gagttccgag tgcgcgtgca gttcggcgcc gccgagcacg
     4201 gcgcgcgtgg cgcggtacaa atcctcgagc agcttgcctt tccacgcgtt ccacaccttg
     4261 gggctggtgc cgcggatatc ggcgacggtc agcagataca gcgcggtcaa ccggcgctcg
     4321 gtcttgacca tcgatgcaaa ctgcttgatg acttccggat cgctcgtgtc ctgcttttgc
     4381 gcgacctggc tcatggtcag atgttgttcc acgagccaca caaccaggtc gctgtcgtcc
     4441 gcgccaacgt cgtgttcacg gcaaaagcgc cgcgcatcca ccgtgccgag cacggagtga
     4501 tcgccgccgc ggcccttggc tatatcgtga aaaagcgccg cgatatacag cacccacggg
     4561 cgctcgaaat tgcccatcag ctggctgcag aacgggtatt catgtgcatg ttcggccatc
     4621 gcgaagcgcc gcatgttgcg cagcaccatc aggatgtgct gatcgaccgt atagacgtga
     4681 tacaggtcgt gctgcatctg gccgacaatg cgccggaagt tgagcaggta acggcccagc
     4741 acgcttgtct ggttcatcag ccgcagcgcg tgcgtgatgc cgtcgggctg cttcaggatc
     4801 tccatgaaca ggcgccggtt ctcggggtcc cgccgccagt cgcggcccat caggtcacgc
     4861 gcgttataga ttgcccgcag cgttcgggcg gacaaaccct tcacgccgcg cgtctgttcg
     4921 tacagcagga acgcttcgag aatagcgttg ggctcgcgtt cgaaaacgtc gtcgctggcg
     4981 atttccagca tgccttgctt ctcgacgaaa cgctcgttga gcacgcgcgt aatgccgctc
     5041 gtcgatggaa acagttgcgc gtccacgttc tggatcagga tggtggcaag ctgcgtgacg
     5101 gccttcgcgg cccagtagta gcggcgcatg agctgttcac tcgcgcgctt ggacgatgta
     5161 gctttgtagc cgagactttc ggccagcgcc gtctgcaagt cgaagacgag aatgtcctgg
     5221 cggcgccgcg caatcacatg cagccgcgcg cgcagggtct tcagaaagcc ttcgttacgg
     5281 cgcagttcgc tcgcttcccg ttcggtgatg aggccacgcg aatccagttc gcgccagctg
     5341 ctgccgaaac ctgccgcgcg cgagatccat aaaataagct gcaagtcgcg caggccgccg
     5401 ggactttcct tgatattcgg ctcgaggctg tacggcgtgt cctggtactt cgcgtgccgc
     5461 tggcgcattt ccaggacttt cgcctggaag aacgcacgcg gatcgagcgt ttgccgatac
     5521 cgcccctcga acgtctcgaa cagtttctca ctgcccacta tgcgccgcgc ttccagcagc
     5581 gaggtctgga tggtgatgtc gttgctcgct tcatcgatac attgcgacac cgaacgcacg
     5641 ctgctgccga tctcgagtcc gagatcccac gccatgccga tcagccgctc gattcgcgcg
     5701 gccagtgtca ccgagtgatc gagtgtttcc tcatcgggca agaggacgag gatatcgacg
     5761 tccgagtagg gcgcaagctc gccgcgccca taaccgccga ctgcaatcaa cgccgccgat
     5821 ttcggcatct cgcaggcttc ccaggcgcct cgaagcgcat cgtcggtggc gcgcgcgagg
     5881 gcgtgcatca ggccatc
//
LOCUS       DATASH010000740         3352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_515815,
            whole genome shotgun sequence.
ACCESSION   DATASH010000740 DATASH010000000
VERSION     DATASH010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515815"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..125)
                     /locus_tag="VHV99_19735"
     CDS             complement(<1..125)
                     /locus_tag="VHV99_19735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HEX3637672.1"
                     /translation="MQPHINLPAPYMPHAGLIAAFDVCPLIAIMRGVTPADAAEHG"
     gene            complement(162..1385)
                     /locus_tag="VHV99_19740"
     CDS             complement(162..1385)
                     /locus_tag="VHV99_19740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017777072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HEX3637673.1"
                     /translation="MPAVTKVAANANGSATASGSATAIGSAAANGSAAANGSAPANGS
                     AAAIGSAAANGSAPANGSAAAIASPTANGSATADLQHAALIALDWGTTSLRAYLYDTA
                     GEVVATRASTAGIMNLPRSAEQGGFDAAFDDACGAWLGQAPAVPVIAAGMVGSAQGWV
                     EAPYVDAPASADALVAGIVRVKAACGATLHIVPGVLQRGELPNVMRGEETQIFGALGQ
                     DTGAMDSSKRSLIGLPGTHAKWAVVQAGRIERFHTFMTGEVFAALREHTILGRTMVTP
                     DRPDTDAFLHGVNIARDKGQSGLLATVFSARTLGLTGQLSREQQPDYLSGLLIGHELA
                     GLEAALMQQQTSLAGQHLRLIGNEALCERYRVALAQFGCTHADLVKHATERGLWRVAT
                     QAGLVNPAPQAARAS"
     gene            complement(1403..2380)
                     /locus_tag="VHV99_19745"
     CDS             complement(1403..2380)
                     /locus_tag="VHV99_19745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX3637674.1"
                     /translation="MNKAMPTHAAGASKADLEPQADHATRAVPQPAANAAKAHRTSAQ
                     PAVLNEIALPSTLLDVTPQQAGTQTLLRGLAILEAAAAGVRDLRTFGAALGTTRSTTH
                     RLVSSLVQARYLRQVQGGYLLGPKLIELGTIALEQMPLTAVARQHLESLAEQTLDTIH
                     LGVRDGDDVLYIDKIPGTRGLEMRSRVGHRMPLASTGIGKAMMLDLTPDVWQSLFEAS
                     RRALAGVSFKPDNRPDQQTFMQRMTNYAAGGYTLDLEENEASIRCVAAPVRDASGSVV
                     AALSVASTIPYMSLERMDELIPVVQREARAISEELGWRTPQPATRRIKR"
BASE COUNT          493 a         1012 c         1166 g          681 t
ORIGIN      
        1 ccgtgctcgg cggcatcagc cggcgtcacg ccgcgcatga tcgcgattag cggacacacg
       61 tcgaacgcgg cgatcaaacc cgcgtgcggc atgtacgggg cgggcagatt gatgtgaggt
      121 tgcatgtcgg ttccttgttt catgagcggc ggcgcggccg gtcagctggc gcgcgccgct
      181 tgaggcgccg gattgacgag ccctgcctga gtggcgacgc gccataggcc gcgctcggtg
      241 gcgtgcttca ccaggtccgc gtgagtgcag ccgaattgcg cgagtgccac gcgatagcgt
      301 tcgcacagcg cttcattgcc gatcaggcgc aagtgctgcc cagcgagcga ggtttgctgt
      361 tgcatgagcg cggcttccag accagccagt tcgtgcccga tcagcaggcc cgacagatag
      421 tcaggctgct gttcgcgcga aagttgtccg gtcaggccga gcgtgcgggc gctgaagacg
      481 gtggccagca agcccgactg ccccttgtca cgggcgatgt tcacgccgtg caggaatgca
      541 tcggtgtccg gacgatccgg tgtgaccatc gtgcggccga ggatggtgtg ctcgcgcaat
      601 gcagcgaaca cctcaccggt catgaaggta tgaaaacgtt cgatgcggcc tgcctgcacg
      661 acggcccatt tcgcgtgggt gccaggcagg ccgatcagcg agcgcttgct gctgtccatg
      721 gcgcccgtgt cctgaccgag tgcgccgaag atctgggttt cttcgccgcg catcacgttc
      781 ggcagttcgc cgcgctgcag gacgcccggc acgatgtgta gcgtcgcgcc gcacgcggct
      841 tttacgcgga cgatgccggc caccagcgca tcggcactcg ccggcgcgtc gacatacggt
      901 gcttcgaccc agccttgcgc gctgccgacc attcctgccg cgatcaccgg cacggcgggc
      961 gcctggccga gccaggcgcc gcacgcgtcg tcgaaggcgg cgtcgaagcc gccttgctcg
     1021 gcgctgcgcg gcaaattcat gatgccggcc gtcgatgcgc gcgtcgccac cacctcgccg
     1081 gccgtgtcgt acagatacgc gcgcagcgac gtcgtacccc agtcgagcgc gataagcgct
     1141 gcgtgttgga ggtcggcggt ggcgcttcca ttggcggtgg ggcttgcgat ggcggcggcg
     1201 cttccgttgg cgggggcgct tccgttcgcg gcggcgcttc cgatggcggc ggcgcttccg
     1261 ttggcagggg cgcttccgtt cgcggcggcg cttccgttcg cggcggcgct tccgatggcg
     1321 gtggcgcttc cgctggcggt ggcgcttccg ttggcgttgg cggcaacttt agtaactgcc
     1381 ggcatggggg ccgccgcttg cttcatcgct tgatcctgcg ggttgccggt tgcggggtgc
     1441 gccagccgag ttcttccgag atcgcgcgcg cttcgcgctg cacgaccgga atcagttcat
     1501 ccatgcgttc cagggacata taaggaatcg tgctcgccac cgacagcgca gccacgacgg
     1561 agcccgatgc atcgcgcacc ggtgccgcaa cacaacggat cgacgcttcg ttttcctcga
     1621 ggtcgagggt gtagccgccg gccgcgtagt tggtcatgcg ctgcatgaag gtctgctggt
     1681 cggggcggtt atccggcttg aagctgacgc cggcgagcgc gcgacgcgat gcttcgaaaa
     1741 gcgactgcca gacgtccggc gtgaggtcga gcatcatggc tttgccgatc cccgtcgacg
     1801 ccagcggcat ccgatgaccg acgcgcgagc gcatttcgag gccacgcgtg ccggggatct
     1861 tatcgatgta caggacgtcg tcgccgtcgc gcacgccgag gtggatcgtg tcgagcgtct
     1921 gttcagcgag cgattcgaga tgctgacgcg cgacagcggt gagcggcatc tgttcgagcg
     1981 cgatggtgcc gagttcgatc agcttcgggc caagcaggta gccgccttgc acctgtcgca
     2041 gatagcgcgc ctgcacgagg ctgctcacca gccgatgggt ggtgctgcgg gtcgtgccaa
     2101 gcgccgcgcc aaaggtgcgc agatcgcgca cgccggcggc ggcggcttcc agaatcgcca
     2161 gaccgcgcag cagcgtttgc gtgccggctt gttgcggggt gacgtcgagc agcgtgctcg
     2221 gcaaggcgat ttcgttgagc acggccggtt gcgcgctggt gcggtgagct ttcgcggcgt
     2281 tggcggccgg ttgcggcacg gcgcgggtcg cgtggtcggc ttgcggctcc agatcggctt
     2341 tggacgcgcc ggcggcgtga gtaggcatcg ctttgttcat ggcgatctgc ttttgggtgg
     2401 tttttttgcg cttacagcgc ggggccagag aggaggcctg ttttgctttt gctcgctgcg
     2461 ccaggcgtgt tgccggtggc gcgggcaggc ggatactgcc tttgggtatt aggcatggtt
     2521 tggctttgtc tccggttttt tttgctgtcg ctggccgcgg ccgattttgc tggctcgtgg
     2581 ggcgggttgg tttggattgt agggcggttt ttgggaaagc tccaatatgt gaatgtttga
     2641 ttcatataat gggatttttc cctgcaggag gcttggggcc cttccttgat ttgtttgcct
     2701 cctcggcgcg ctttatggct gtgcttttat ggcgtgggcc tttccttgaa ttgatattgg
     2761 tctattagcg tcgcccctgt gcggggcggc acctactttt ctttgcctgc cgcaaagaaa
     2821 agtaggcaaa agaaagcggc tcacaccgcc agctcataag tgggcccccc gcacagctac
     2881 ggtagtggcg catctggaat cggtgccctc gcgcattcga cgtgagtgac caggcagtca
     2941 tacttcccgc ctcgcactgc gtgctcgccg gaagggttta tcaaaaaccc ccgggcttcg
     3001 tttgggcacg gtgggggccg tcggcttcgc ctcggcgaag cgcccgaacg acagcacgag
     3061 agagacaaac tcgaagagat aatataagcc gagccaccgc gcgaaatgag ccgtgagcgc
     3121 gaaaccgccg tgatcggctt tacgaactac gcttcggcgc gcgcagcgcc gccggaagta
     3181 tgacggcctt gtcactgacg cggagtgtgc gagggcacgg attccagatg caccactacc
     3241 gcgactgtgc gggggacccg cttatgcgct ggcggtttga gccgctttct tttgcctact
     3301 tgtatattcc agtccgtggc gccgacccga tcagttgtca ccacacggac ag
//
LOCUS       DATASH010000741         3038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4275178,
            whole genome shotgun sequence.
ACCESSION   DATASH010000741 DATASH010000000
VERSION     DATASH010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4275178"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /gene="accD"
                     /locus_tag="VHV99_19750"
     CDS             <1..756
                     /gene="accD"
                     /locus_tag="VHV99_19750"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEX3637675.1"
                     /translation="RNDVEANLHVCPKCDHHMRIGARERLDGLLDPEGRYEIGQEIVP
                     VDALKFKDSRKYPDRLKEAMDDTDETDAMVVMGGAIHTLPVVVACFEFSFMGGSMGSV
                     VGERFARGAQNALEQKVPFICFTASGGARMQESLLSLMQMAKTTAMLTKLAEAKLPFI
                     SVLTDPTMGGVSASFAFLGDVVIAEPKALIGFAGPRVIEQTVREKLPEGFQRAEFLLT
                     KGAVDMIVDRRKLREEIAQLMALLSHQPADAVA"
     gene            935..2245
                     /gene="folC"
                     /locus_tag="VHV99_19755"
     CDS             935..2245
                     /gene="folC"
                     /locus_tag="VHV99_19755"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010089192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEX3637676.1"
                     /translation="MTSFPTLDAWLTHLESAHPVGIDMGLGRITQVRDAMQLSFACPV
                     ITVGGTNGKGSTCAILESILLRAGFTVGCHTSPHLLSFNERARVNGEMASDADLLPHF
                     EAVEAARLSLVEPVTLTYFEFTTLAIMSLFASRGLDAVIFEVGLGGRLDAVNILDTDC
                     AIITSIDIDHTEYLGDTREKIAFEKAGIFRPGKPAICADPVPPQTLIDYAEKIGAELW
                     LFGRDFRYEGQAGSERQQWSYVGPTMRRSSLAYPALRGANQLINTSAALAGLEALRDR
                     LPVSAQDIRLGLANVELPGRFQVLPGKPSIVLDVGHNPHAAAVLAQNLGNMGFFPYTY
                     AVFGAMRDKDIGGVLSHLKGEIDHWCVTGLPTPRAASAEELEAALREQGVSEGSDSSV
                     TRYATPAAAFQDALKRASENDRIVVFGSFYTVAGVMAYRKSQQH"
     gene            2275..>3038
                     /locus_tag="VHV99_19760"
     CDS             2275..>3038
                     /locus_tag="VHV99_19760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX3637677.1"
                     /translation="MGIFSFGKKGDAPSRRGANTSSTRAARGERVERRTRRTERTVDA
                     DAMLLDPTLPEKQRARRRLVGAIALVVAAVVILPMVLDSHPKPVTDDISIEIPSRPAP
                     KVARTDEDTQAGVAPDNPVPDAAVAASGLAPTTAATQGQSGAGKQQSAATSPATAAKP
                     AVKPQAPAAVAANTAPAAPIAQAPAKPAKPSNAPATQSAANVAAQNEDSANTAAASAD
                     ANSGTPASPPGSRFAVQLGAFANDANARNWAAKLKAA"
BASE COUNT          594 a          989 c          949 g          506 t
ORIGIN      
        1 cgcaatgacg tcgaggccaa tctgcatgtt tgcccgaagt gcgaccatca catgcgcatc
       61 ggcgcgcgtg agcggctcga cggcctgctc gatccggaag gccgctacga aatcggccag
      121 gaaatcgtgc cggtggacgc gctcaagttc aaagacagcc gcaagtaccc ggatcgcctg
      181 aaagaggcga tggacgacac cgacgaaacc gacgcaatgg tcgtcatggg cggcgccatc
      241 cataccctgc cggtggtggt cgcgtgcttc gagttttcgt tcatgggcgg ctcgatgggc
      301 tcggtggtcg gcgagcgttt cgcgcgcggc gcgcagaacg cgctcgaaca gaaagtgccg
      361 tttatctgct tcaccgcttc gggcggcgca cggatgcagg aaagcctgct ctcgctgatg
      421 caaatggcga agaccacggc catgctgacc aagctcgccg aagccaagct gccgttcatt
      481 tccgtgctga ccgatccgac catgggtggg gtatcggcga gtttcgcgtt cctgggcgac
      541 gtggtgatcg ccgagccgaa ggcgctgatc ggcttcgccg gcccgcgcgt gatcgaacaa
      601 accgtgcgcg aaaagctgcc ggaaggcttc cagcgcgccg agttcctgct gacaaagggc
      661 gcggtcgaca tgatcgtcga ccgtcgtaag ctgcgtgaag agattgcgca attgatggcg
      721 ctgttgagcc atcagccggc agacgcagtc gcttaaaagt ttgaattaat ctggccgcgc
      781 cttttcgcgc gcccgccgtc ggcacagcag cccttcagca cggcggcaat catcgaagca
      841 gccttcaaaa aacagcgcgc cgcgagagtc atcaagcggc gcgctgtcat ttccgggata
      901 atcacgatct cgcaacgcag cgcagattca ctcgatgacc tcattcccca ccctcgacgc
      961 gtggctcacg caccttgaat ccgcgcatcc ggtcggcatc gacatgggtt tgggccgtat
     1021 cacgcaggta cgtgacgcga tgcagttgtc gttcgcgtgc ccggtcatca cggtcggcgg
     1081 tacgaacggc aagggttcga cctgtgcgat tctcgaatcg attttgctgc gcgcgggctt
     1141 cacggtcggc tgtcatacgt ccccgcatct gctgtcgttc aacgaaaggg cgcgcgtgaa
     1201 cggtgaaatg gcgagcgacg cagacctgct gccgcatttc gaagccgtcg aagccgcccg
     1261 cctgagcctc gtcgaaccgg tcacgctgac ctacttcgaa ttcacgacgc tggcgatcat
     1321 gagcctgttc gcctcgcgcg gactggacgc ggtgatcttc gaagtcggcc tgggcggccg
     1381 tctggacgcg gtcaatatcc tcgatacgga ctgcgcgatc atcaccagca tcgatatcga
     1441 ccacaccgaa tatcttggcg acacacgcga gaaaatcgcc tttgagaaag ccggcatttt
     1501 ccgcccaggt aagccggcga tctgcgccga tccggtgccg ccgcagacgc tgatcgatta
     1561 cgctgagaag atcggcgccg aactgtggct attcggccgc gatttccgct acgaaggaca
     1621 ggcaggcagc gagcgccagc aatggagcta cgtgggcccg acaatgcgcc gttcctcgct
     1681 cgcctatccg gccttgcgcg gcgccaatca gttgatcaac acatccgcgg cgctggccgg
     1741 tcttgaagcg ctgcgcgacc gtttgccggt gtcggcgcag gatatccggc tcggcctcgc
     1801 caacgtcgaa ctgccggggc gctttcaggt gctgcctggc aaaccgtcca tcgtgctcga
     1861 cgtcggccac aatccgcacg ccgcggcggt gttggcgcaa aatctcggca atatgggctt
     1921 tttcccatac acctacgccg tgttcggcgc gatgcgcgat aaggatattg gcggcgtact
     1981 gtcgcacctg aagggtgaaa tcgaccactg gtgcgtaacc ggtctgccga ctccgcgcgc
     2041 ggcttcggcg gaagaactcg aagcggctct gcgcgagcaa ggcgtgagcg agggcagcga
     2101 cagcagcgtg acgcgctacg caacgccggc cgccgctttc caagacgcgc taaaacgagc
     2161 gtcggagaat gatagaatcg ttgttttcgg cagtttctat acggtagcag gcgtaatggc
     2221 ctaccgtaaa tcgcagcaac actgaacggg cgccaggctc gaaccaagcg attcatggga
     2281 attttctcgt tcggcaagaa aggcgacgcg cccagccggc gcggcgcaaa caccagttcc
     2341 actcgggccg cccgtggcga gcgcgtggag cggcgaaccc gccgcacgga gcgcaccgtg
     2401 gatgccgatg cgatgctgct cgaccctacg ctgccggaaa agcagcgtgc gcggcgcaga
     2461 cttgtcggcg cgatcgcgct ggtcgtcgca gcggtggtga tcctgcccat ggtgctggat
     2521 tcccatccca agcccgttac tgacgacatc tccatcgaaa ttccaagccg tcccgcgccg
     2581 aaggtcgcca ggaccgacga agacacgcag gccggtgtag cgccggataa cccagtgcct
     2641 gacgcggcag ttgccgcttc cggccttgcg ccgacaacgg cagccacgca aggccagtcc
     2701 ggcgccggta agcagcagag cgcggctacg agcccagcga ccgcagccaa accggccgtg
     2761 aaaccgcaag cgcccgccgc cgtcgccgcc aatacggcac ccgcagcgcc catcgcgcaa
     2821 gcgcccgcca aaccggccaa accgagcaac gcgcccgcca cgcaaagcgc cgcgaacgtt
     2881 gcagctcaaa acgaagacag cgcgaatact gccgccgcca gcgcggacgc gaattccggc
     2941 acgccggcgt cgcccccggg tagccgtttc gccgtccagc tcggcgcatt tgcgaacgac
     3001 gccaacgcgc gcaattgggc cgccaagttg aaagcggc
//
LOCUS       DATASH010000742         8393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6648691,
            whole genome shotgun sequence.
ACCESSION   DATASH010000742 DATASH010000000
VERSION     DATASH010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 8393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8393
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6648691"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1217
                     /locus_tag="VHV99_19765"
     CDS             <1..1217
                     /locus_tag="VHV99_19765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3637678.1"
                     /translation="MMRRADWLVDVGPAAGEAGGHVVYSGPPAGLAKVEASQTRRHLF
                     APPAPVDRTPRSPGGWLRLANITRNNLRGLDVAFPLGCFTTVTGVSGSGKSSLVSQAL
                     PELVAGRLGRIIESPDDDEQDPLFATETTPTSGHIAEGMDTLRRLVRVDQKPIGRTPR
                     SNLATYTGLFDNVRKLFADTPLARKRRYSAGRFSFNVAQGRCPTCEGEGFVSVELLFL
                     PSVYAPCSTCHGTRYNPQTLEITWRDKNIAEVLHLSVDAACDFFADEAGVMRALKVLR
                     DIGLGYLRLGQPATELSGGEAQRIKLATELQRAQRGDTLYILDEPTTGLHPVDVDRLI
                     VQLQGLVDAGNTVVVVEHDMRVAAQSDWVIDVGPGAGDAGGTIVASGTPAEVARAEQS
                     RTATYLKQRLAA"
     gene            1323..1646
                     /locus_tag="VHV99_19770"
     CDS             1323..1646
                     /locus_tag="VHV99_19770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637679.1"
                     /translation="MQQSLSSLDFTLAPEWVAPLDVVSESARPFPYYMFADGHQARIH
                     GQVAGADKPTAYSSPVRIAAPNAQKTSTESAAFRAERGAFYLRKILQKVFPLVNEFAQ
                     SFVYK"
     gene            2033..2182
                     /locus_tag="VHV99_19775"
     CDS             2033..2182
                     /locus_tag="VHV99_19775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637680.1"
                     /translation="MDDKPTKIPTPDKVFSPDPEPAGVEFLGAELPEHVRAFFDEQRK
                     SCDSK"
     gene            2464..3060
                     /locus_tag="VHV99_19780"
     CDS             2464..3060
                     /locus_tag="VHV99_19780"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018434466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEX3637681.1"
                     /translation="MNRSYRFAASCALALLTALVIAPTPAAADGDDTALTSLIALASQ
                     RLALAEPVARWKWANHQAITDTPRENALLADVEKRAVAANVDPAFARAFFQDQIDASK
                     DVQNALFASWRSTRPPEGPAPDLAASTRPQLDRLTQSLVAGLARVQPLRAAQDCPSRV
                     AQSLANWKLLTRYDSAHSSALTRALGHVCEAGGVGATG"
     gene            complement(3204..4358)
                     /locus_tag="VHV99_19785"
     CDS             complement(3204..4358)
                     /locus_tag="VHV99_19785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HEX3637682.1"
                     /translation="MSEQLLPEPALDVPIVFVDLETTGGSTSEHRITEVGVVEIGQAG
                     VSRWSSLVDPQQPIPSFIQQLTGITNAMVRGAPTFDAIAPALYERLNGKLFVAHNASF
                     DRGFLRGEFRRAGFAFDPDVLCTVRLSRALFPAEKRHGLDALVERHGLVPSDRHRALA
                     DADLIWQFWQRLHGGLVPPDVLQAQIERTTRRYRLAGDITEDLLDTAPPGCGVYVFYG
                     EGDLPLYVGRSVRVRQRLRSHLTGERRSSKDMRLAQQVRRVEWRATGGELGAMLAEAQ
                     WIATLRPGHNRLPRVTKSDPVDAPWPFEGPIVFEEREDASHARAFHIVDRWRYVGHAP
                     SLAQAAALHASSAVGPFELSTYRLLQSHLARGLRVMPLSVSSGTPVPSLA"
     gene            complement(4716..4919)
                     /locus_tag="VHV99_19790"
     CDS             complement(4716..4919)
                     /locus_tag="VHV99_19790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006400778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX3637683.1"
                     /translation="METGIVKWFNDAKGFGFITSDSGGEDLFAHFSEIRTEGFKSLKE
                     SQRVSFEVKAGPKGKQAANIQPL"
     gene            5361..5609
                     /locus_tag="VHV99_19795"
     CDS             5361..5609
                     /locus_tag="VHV99_19795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637684.1"
                     /translation="MQFNGLGRFIAWLTQSHVKTVPAQSGPAAASSSVFEFDPMWHGD
                     HWQNLLSAPMDARRYVMEDWSASLATDWNEADGTAPVH"
     gene            6141..7289
                     /locus_tag="VHV99_19800"
     CDS             6141..7289
                     /locus_tag="VHV99_19800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX3637685.1"
                     /translation="MKKTLMVTALTGVFATAAHAQSSVTLYGLIDAGITYTNNQHGHS
                     NWQETSGSVNGSRWGLRGSEDLGGGLKAIFVLENGFGINDGTLKQGGREFGRQAFVGL
                     TSDQFGAVTLGRQYDSMVDYVGPLALTGTQYGGTQFAHPFDNDNLNNSFRINNSVKYQ
                     SANYGGFKFGALYGFSNQADGFANNRAYSAGASYNWGGLNIAAAYLQLNSNGATGAAA
                     AFNSGGAVSSDNTFFAGRQQTWGAGANYAFGPATVGLVYSQTNLGGLAGIGAGASGQT
                     AGIGGFGGDSAHFQNFEANARYAVTPAVSIAGSYTYTRASLGGTRPHWNQFNLQTAYA
                     LSKRTDVYLQGEYQQVNEGAIVDADINGLGASSNNKQIAVTAGLRHRF"
     gene            complement(7493..8062)
                     /gene="greB"
                     /locus_tag="VHV99_19805"
     CDS             complement(7493..8062)
                     /gene="greB"
                     /locus_tag="VHV99_19805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HEX3637686.1"
                     /translation="MNKAFVKESSEENDDDLDVVQPEIPPGTKNYITPAGYRRMRDEL
                     LHLIDVDRPEVVKLVSWAASNGDRSENGDYIYGKRRLREIDRRIRFLTKRLDIAEVVD
                     SSKQENVDQVFFGATVEYATADGEVHTLTIVGVDEVDLDVGHVSWISPIARALLKAKI
                     DDTVTLYTPAGPQPIEILDVKYPPPDTGG"
     gene            8183..8259
                     /locus_tag="VHV99_19810"
     tRNA            8183..8259
                     /locus_tag="VHV99_19810"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8217..8219,aa:Arg,seq:acg)
BASE COUNT         1756 a         2548 c         2424 g         1665 t
ORIGIN      
        1 agatgatgcg gcgcgccgac tggctggtcg acgtcggtcc cgcagccggc gaagcaggcg
       61 gtcatgtggt ctatagcgga ccgcccgctg gactcgccaa ggtcgaagcg tcgcaaacgc
      121 gccggcatct gttcgcgccg cccgcgcctg tcgaccgcac gccgcgctcg cctggcggct
      181 ggctgcgcct tgccaatatc acgcgcaaca atctgcgtgg gctcgacgtc gcgtttccgc
      241 tcggctgctt cacgaccgtc accggtgtgt cgggctccgg caaatcgagc ctcgtgagcc
      301 aggcgctgcc ggagctggtc gcaggccgcc tgggacggat catcgaaagc ccggacgatg
      361 acgaacagga tccgctcttc gccaccgaaa cgacgccgac cagcggccat atcgccgaag
      421 gcatggacac gctgcgccgg ctcgtgcgcg tcgatcagaa accgatcgga cgcaccccgc
      481 gctcgaatct cgccacgtac acgggcctgt tcgacaacgt gcgcaagctg ttcgccgaca
      541 cgccgctcgc gcgcaagcgc cgctacagcg cgggccgctt ctcgttcaac gtcgcgcaag
      601 gccgctgccc gacatgcgag ggcgaaggct tcgtgagcgt ggagctgctg tttctgccga
      661 gtgtctatgc accctgctcg acctgccacg gcacccgcta caacccccag acactggaaa
      721 tcacgtggcg cgataaaaac atcgcagagg tgctccacct gagcgtcgac gccgcctgtg
      781 atttcttcgc cgacgaagcc ggcgtgatgc gcgcactaaa ggtgctgcgc gacatcggcc
      841 tcggctattt gcggctcggc cagccggcca ccgaactgtc gggcggcgaa gcgcagcgca
      901 tcaaactcgc caccgaactg cagcgcgctc agcgcggcga cacgctgtac atcctcgacg
      961 aaccgaccac cggcctgcat cccgtcgacg tggaccggtt gatagtgcaa ctccagggtc
     1021 tcgtggacgc gggcaacacg gtggtagtgg tcgaacatga catgcgggtc gccgcgcaaa
     1081 gcgactgggt catcgacgtc ggcccgggcg cgggcgacgc cggtggcacg atcgtggcaa
     1141 gcggcacgcc cgctgaagtg gcacgcgctg agcaaagccg cacagcaacc tatctgaagc
     1201 aacggctggc ggcctgaagt agaagccgcg cgtgagattt accggacacc gcgcttctcc
     1261 ctcaggtagt cgatcagcgc gcgcagcttg aacggcgtct aggcgcggct cgcccggaac
     1321 agttgcagca gtcgctcagc tcgctcgatt tcacgctcgc gccggaatgg gtcgcgccgc
     1381 tcgatgtggt ctcggaatct gcccgtccgt tcccgtacta catgttcgcc gacggtcatc
     1441 aggcgcgaat tcatggacaa gtcgcaggcg ccgacaaacc cacggcctat tcctcgccag
     1501 tgagaattgc agcgccgaac gcgcaaaaaa cgtcgacgga gagcgctgcg tttcgtgcgg
     1561 aacgcggcgc cttttatttg cgaaaaatac ttcaaaaagt attcccgctc gtcaacgaat
     1621 tcgcgcaatc gttcgtttac aaataattcg ggggctgaat caaggctgaa ccggccgata
     1681 attaacgaac gttcggccta actcagagaa aatacttcga tgcggcggcg cacaaaaatc
     1741 gcgatgattt gttcatatca acacatttga accgatgctg ctgtgacgcg acagagataa
     1801 tgcgccaaag aggcgcgggg caaggctggg aatggaacag cggaggatat cggactgttt
     1861 tgattggcgg cggcgaacgc agccgcgtgc agtcaaccga taaattctat tcaaacatgt
     1921 tggtcccatg ggggcctgta ccgttaaagc aacacaccac ctgtgagccg catcagactc
     1981 tggtgcaaaa cggggaccga ggcagataat cggtttgtaa aggagaatga tcatggatga
     2041 caaaccaacg aagattccga ccccggacaa ggtttttagc ccggatccgg aacccgctgg
     2101 cgtcgaattt ctgggtgctg aactgcccga acatgttcgg gcatttttcg acgaacaacg
     2161 caaatcgtgc gattcgaagt aagtgcgcaa tggtgcgatg caacatcggc gtccgcgcgt
     2221 ctggcacgcg gcgcagattc gcacgcgcct aaacaatcgt tttgcgcgcc gttcgtaacg
     2281 cgaaccgggg ttatttgacc gcggcgccgg gcattgcccg gcgttcgatt gcgcgcgcct
     2341 ttaacgagcg gcgttagtag cctgggtatt ttgttttcga tcgttccacg cgccccgccc
     2401 gcgtagcaca tcgtctattc ttctggtttt tctcccgcgg ccgatcggcc acccctgccc
     2461 ccgatgaatc gctcgtatcg ctttgcagcg tcatgtgcgc tcgcgttact caccgcgctt
     2521 gtgatcgcgc ccacccccgc cgctgcggat ggcgacgaca ccgcgcttac cagtctgata
     2581 gcgctggctt cgcagcgcct cgccctcgcg gagcccgttg cgcgctggaa gtgggccaac
     2641 catcaggcga ttacggatac gccacgtgag aacgcgttgc tcgccgatgt cgaaaaacgc
     2701 gctgtagccg caaatgtcga tccggcgttt gcacgcgcct tcttccagga tcagatcgac
     2761 gccagcaagg acgtgcaaaa cgcgctgttt gccagctggc gcagcacccg gccgcccgaa
     2821 ggtcccgcac ccgacctggc cgccagcacg aggccgcagt tggaccggtt gacgcagtca
     2881 ctcgtggccg gcctcgcgcg tgtgcaaccg ctgcgcgccg cgcaggattg tccgtcgcgc
     2941 gtcgcgcaat cactggccaa ctggaaattg ctgacgcgct acgactcggc ccattcgagc
     3001 gcgttgacgc gagctctggg ccatgtgtgc gaagcaggtg gagttggcgc gaccggttga
     3061 gcggtgagcg gtgagcggtg agcggtgagc ggttgagcgg ttcggtatgc ggtgaacgcg
     3121 gcaccgagag gccccgctgg tccgccaccc tcaggcggag cttgaattcg ctctgagcaa
     3181 cgggacactc cgcaaaagcc gtgtcaggcc agactcggca caggcgtgcc gctcgagacg
     3241 ctcagtggca tcacacgcaa accgcgcgcc agatggctct gcagaagacg ataggtcgac
     3301 agctcgaacg gccccactgc gctcgacgca tggagcgccg ccgcctgcgc gagtgaaggg
     3361 gcatgtccga cgtagcgcca ccgatcgaca atatgaaaag cgcgcgcgtg cgatgcatcc
     3421 tcacgctctt cgaatacgat cggaccttcg aacggccagg gcgcatcgac cggatcgctc
     3481 ttcgtgacgc gcggcaaccg gttatgacca ggccgcaacg ttgcgatcca ctgcgcctcc
     3541 gcgagcatgg cgcccagttc gccgcccgtc gcccgccact cgacacgccg cacttgctgc
     3601 gcgagccgca tatccttgga cgagcgccgc tcacccgtca aatgcgaacg caagcgctga
     3661 cgcacacgta cgcttcgccc gacgtatagc ggcagatcgc cttctccata gaacacgtag
     3721 acgccgcatc cgggcggcgc ggtatcgagc agatcttcag tgatgtcgcc ggcaagccgg
     3781 tagcgccggg tggtgcgctc gatttgggcc tgcaagacat ccggtggcac gagaccgccg
     3841 tgcagtcgct gccagaactg ccagatcaga tcggcgtcgg cgagcgcgcg gtggcgatcg
     3901 gacgggacga gaccatgacg ttccaccaac gcatcgagcc catggcgctt ttccgccgga
     3961 aacaacgcgc gcgacagacg cacagtgcac agcacgtccg gatcgaacgc gaacccggcc
     4021 cggcgaaact ccccgcgcaa aaagccgcga tcgaaactgg cgttgtgcgc cacaaacagt
     4081 ttgccgttca ggcgctcgta caaggccggc gcaatggcgt cgaaggtcgg cgcgcctcgc
     4141 accatcgcat tcgtgatgcc ggtgagttgc tgaatgaagg acggaatcgg ttgctgaggg
     4201 tcgaccaggc tgctccagcg cgacacgccg gcctgtccta tttcaactac gccgacttcg
     4261 gtgatgcgat gctcactggt cgagccgccg gtggtttcaa ggtcgacgaa gacgatcggc
     4321 acatccaggg ccggttccgg cagaagctgt tcagacatgg aaaggagtac tttggacgcg
     4381 tggctttgcg gcaagtatgc accagttagc cgccgcacac ccggccagca ttgttttgcc
     4441 ctggtcactg cgcggtgcaa agactgcagt cctgcacgcc ccatgaaccg ttcgtaaaac
     4501 cggggggatt attatatttt atgacgttga ttttttaatt tcaaaaaaac acttttttat
     4561 cttccatcct tcgcagatta attcggaccg ggtagttaat agcttcagca cggtaatcat
     4621 gataaacgac cgtaaaaaaa gccctcgcat ccagcgacgg ctttttggag caggctgccc
     4681 ggcgctgcac ccgcaacgga gcagcgcgac gcgacttaga gcggctgaat atttgccgcc
     4741 tgcttgccct tcgggcccgc cttgacctcg aaagaaacgc gttggctttc ttttagtgac
     4801 ttgaagcctt cggtgcggat ctccgaaaaa tgcgcgaaca ggtcttcgcc acccgaatcc
     4861 gatgtaatga agccaaagcc cttagcatcg ttgaaccatt tgacgatacc agtttccata
     4921 ttccagtttt cctcgagaat taatcaaaat cgggcatggc cctcacgctg catttcaaca
     4981 tcgtttcatc tacgatcgcg cgtttctgaa gggcttgcac catcatattc gacgattgaa
     5041 attcagcatt gccgttatac gggtaacgca gaatcaacgt caagcgaatt tttcgtccgc
     5101 ccttggcagg ctgcacgata agacaacgcc aaatggatcg aaaatcctgt ccgcatattc
     5161 agtggaaatt gaataatctt tcgcgtcaaa attacccatc tccaactcga cgtgcgaaaa
     5221 atcgggaaaa atttcagcat gtcgtgagaa catgtaacaa aaaatatttt tattaagtgt
     5281 ttgtccgagt tgttgcgccg cactctgcac aggatgatgg aactcagtca caaggagtaa
     5341 atgcgcatca ggagattgcc atgcagttca atggattggg ccgcttcatc gcctggctca
     5401 cccaatcgca cgtaaaaacg gttcccgctc aatccgggcc ggccgctgca tcatcttcgg
     5461 tcttcgaatt cgatccgatg tggcatgggg accattggca aaatctgctc tcggcgccca
     5521 tggacgcccg tcgctatgtg atggaagact ggagcgcctc gctggcgact gattggaacg
     5581 aagcggacgg taccgcgccc gtgcactgac caacgcccac aaatccattg tcgacaggca
     5641 aaaaaagagc gcgactggaa agtcgcgccg acaaaggttt ggagatcttt ttcgtcaatg
     5701 aaaaagtctg cttcggaaag cagaagtttc agtatagcgg tttaatcgat atcgattatt
     5761 agcacaatca acaaccatct attgccgtcg caacaacaat cactttctag gatttactac
     5821 gttgtttttt tgcatcgatt aatgtagcaa atgaacaacg cgtttcagtt tattccgctt
     5881 tcaccctccc ctaataccgc tgcaagcctt tatcagcaag gcttaaacgg tattcgtcca
     5941 tcgttccata tatggtaata ctttattgcg tgaatcggaa ttctcgcttc gcaagcgtct
     6001 atctacactc tgcttaatcg gaaaccggcg cgtttggaat acaacgcgtt cgtcatgcgg
     6061 atttcgcctc tcgcaacgcc cgcgtgaagg tctccttaag cctgggttaa atgcccactc
     6121 tcgctctcgg agttacaaag atgaaaaaga ctctcatggt cacggccctc acgggcgttt
     6181 ttgcaacggc agcgcatgcc cagagcagcg tcacgctgta tggcttgatc gacgccggca
     6241 tcacctatac gaataaccag cacggccaca gcaactggca agaaacgagc ggttcggtga
     6301 acggcagccg ctggggcttg cgcggttctg aagacctggg tggcggtctt aaggcgatct
     6361 tcgtgttgga gaacggcttc ggcattaatg acggcacgct gaaacaaggt ggccgcgaat
     6421 tcggccgcca ggcgttcgtc ggcctgacga gcgaccagtt cggcgccgta acccttggcc
     6481 gccaatatga cagcatggtc gactacgtgg gtccgctggc gctcaccggc actcaatacg
     6541 gcggcacgca gttcgcgcat ccgtttgaca acgacaacct gaacaactcg ttccgcatca
     6601 acaactcggt caagtatcaa agcgcgaact atggcggctt caagttcggc gccctgtacg
     6661 gcttctcgaa tcaggctgac ggcttcgcca acaaccgcgc ttacagcgcg ggcgcatcgt
     6721 ataactgggg cggcctgaac atcgcagcag cctacttgca actgaacagc aacggcgcaa
     6781 ccggtgcggc tgcggcgttc aattcgggcg gcgcagtgtc gagcgacaac acgttctttg
     6841 ctggccgcca gcaaacgtgg ggggcaggtg ctaactatgc gttcggcccg gctactgtcg
     6901 gcctcgtgta ctcgcagacg aaccttggcg gcctggccgg tattggcgca ggtgcgtcgg
     6961 gtcaaacggc gggcatcggc ggctttggcg gcgacagcgc gcacttccag aacttcgaag
     7021 ctaacgctcg ctacgcggtg acgccggcag tgagcatcgc cggttcgtac acgtacacgc
     7081 gcgcaagcct cggcggcacg cgtccgcact ggaaccagtt caacttgcaa acggcgtacg
     7141 cgctgtccaa gcgcacggac gtgtacctgc aaggcgagta ccagcaagtc aacgaaggcg
     7201 ccatcgtgga cgccgacatc aacggcctcg gcgcttcgag caacaacaag caaatcgccg
     7261 tcaccgcagg gctgcgtcac cgcttctaaa gcgattggcc atacctgcag tatcgaggcg
     7321 ccgtctgcgg cgcctttttt tatgccgcgc cgaaagcacg ctttgcaaga gaggttgaga
     7381 tgggctggcg tcagacgtcg aatcggaacg agtcgccggc aattgccttg aaactgccat
     7441 ttcctgagcc gcgcaccctc gcccgactca tacgccgcgc ttgtacattc gcctagccgc
     7501 cggtatcagg cggcggatac ttgacatcca ggatttcgat cggctgcggg cccgccggtg
     7561 tgtacaacgt caccgtatcg tcgattttcg ccttgagcag cgcccgcgcg atcggtgaga
     7621 tccaactcac gtggccgaca tccaggtcga cctcatcgac accgacaatg gtcaaggtat
     7681 gcacctcgcc gtctgccgtc gcgtattcca cggtcgcgcc gaaaaagacc tgatcgacgt
     7741 tttcctgctt gctgctgtcg accacttcgg cgatgtcgag gcgcttggtc agaaagcgaa
     7801 ttcgcctgtc gatttctcgc agacggcgct taccgtagat gtagtcaccg ttttccgagc
     7861 ggtcaccgtt cgaggccgcc cacgacacca gtttgacgac ctcggggcga tcgacatcga
     7921 tcagatgcag cagttcatcg cgcatacgcc ggtagccggc gggcgtgatg tagttttttg
     7981 tacccggggg gatttcgggc tggacgacgt cgagatcgtc gtcattctct tcgctcgact
     8041 ctttgacaaa ggccttgttc atgatggtcc gttaactgca gcaagcgact gcctatcaag
     8101 ggtacacgat cagggcaatg agacaaatcg cagttacatg cttccctttt tgaaaacctt
     8161 tgctataatc tcgtttctgc gtgcggctgt agctcagttg gatagagtac ttggctacga
     8221 accaaggggt cgtgggttcg aatcctgcca gccgcaccac ttatttgagg gcctccctcc
     8281 ggggaggccc tatgttttac cccggtttca tcgcagtatc acctgtttag cgtgcggctg
     8341 tagctcagtt ggatagagta cttggctacg aaccaagggg tcgtgggttc gaa
//
LOCUS       DATASH010000743         4186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_913223,
            whole genome shotgun sequence.
ACCESSION   DATASH010000743 DATASH010000000
VERSION     DATASH010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913223"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VHV99_19815"
     CDS             complement(<1..190)
                     /locus_tag="VHV99_19815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008652495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637687.1"
                     /translation="MKKQKRYQRLTAVAVAVTTLCMSHAAFSLTAAPNKDATAITKQE
                     NAEVYKQLPFDDKQDFEDA"
     gene            complement(341..631)
                     /locus_tag="VHV99_19820"
     CDS             complement(341..631)
                     /locus_tag="VHV99_19820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637688.1"
                     /translation="MSLRGASDAKFQDNNPLTAEAEIRWNVMPRWSVLGFSGVGKAYG
                     CLHSFSDAPTAFGCGTGFRYVTARKLGVTMGTDVAHGPGQNAFYVQAGSAWR"
     gene            complement(826..1797)
                     /locus_tag="VHV99_19825"
     CDS             complement(826..1797)
                     /locus_tag="VHV99_19825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEX3637689.1"
                     /translation="MSRPNRLSASRSLARLCTGALLSAAGVGISQQGWATEGGIGRPI
                     TGQQVTPYAGVVPPTDDWIVSAATIYYEGSLGASKTIPIAGQITAGVDVTAVYTIVNA
                     VKTWGITLGGWNFASSFGVPFQYTNISSFHGRLPNDHNTQFADIFFTPVVAGYHLTKT
                     DHVAFSVQIYAPTGAYSSSRLANAGQNTWTFTPTVAYTKLFPKEEIELSLNYGVEFYT
                     PNNDTHYHNAPVSVLDLLALKRFGSGWGVGVVGGYIQQIGHDSGGLAELTGGNQGHSV
                     GIGPMVTWSGKVQKTPVSASLRWVNEFNVSNRPKGNAVQLSISATFQ"
     gene            complement(1905..3392)
                     /locus_tag="VHV99_19830"
     CDS             complement(1905..3392)
                     /locus_tag="VHV99_19830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HEX3637690.1"
                     /translation="MNKKTATWITAAVLASVAAPILAKTQQDQPVHATATTASVPGPV
                     TGYAVSEEYARLVARQTYFWAWPMVNVYGRLLAMKQVPQPGLNGGVVSVAPPNHLSML
                     RDYIAPQERFVACPNQDVVYGSAILDLNRTPVVVQVPDFGKRFWVYQAVDLRTDGFAS
                     LGKMYGTRPGFYLLAGPDWHGQVPKGIRQVYRSSTGVGSFIPRVFMDDTPEDRVAVQS
                     VIAKIALYPLAEYDGTVKETDWSKLPSFGPAGGAASGNAEEIKWVDPNRFWDELPVVL
                     DETPPQRGEEALYAQARALIAAAQKDPAIKTAVVDEAAKAEADLVTPLFNFNTFGKRL
                     PANWNTISNGAAFGTDYFTRTAVAKSNIFVNKPNETKYFYADADASGQRLDGSAYYTV
                     TFAKDSLPPVKGFWSLTLYNSHHFFSPNAQNRYSLGTKNKNLKFNADGSLTLYVQSTS
                     PADDKLANWLPAPANEAFSLYVRAYWPQQPALDGSWTPPPIRREP"
     gene            complement(3421..>4186)
                     /locus_tag="VHV99_19835"
     CDS             complement(3421..>4186)
                     /locus_tag="VHV99_19835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HEX3637691.1"
                     /translation="ERTAAPPPVAKLDFPKWDEAKATSVDFIAYLNFLLQFTQPSAPS
                     ERELMARFSKIGIAPGAPFDPAALPPEIRAALEAGVAGGKAALADAEKHTTSSFNLFG
                     SRADLKDDYTTRAVAAAMGIYGNTKEEAVYVGTRVNGEHAQLLGAQPYVLHFDKKDLP
                     PAKFFWSMTLYDLPARHLVANPINRYSIGDRTKGLKYGADGSLDIYVQNTSPGADKAS
                     NWLPAPIGAYDLVMRIYGPDQRVLDGAWKFPELQKR"
BASE COUNT          782 a         1299 c         1275 g          830 t
ORIGIN      
        1 gcgcgtcctc gaaatcctgc ttgtcgtcga acggcagttg cttgtagacc tccgcgtttt
       61 cctgcttcgt gatggccgtt gcgtccttgt taggcgcggc ggttagtgaa aaggctgcat
      121 gcgacatgca aagcgtagta actgcgactg cgacagcggt gagccgctga tagcgctttt
      181 gttttttcat cgtcttttcc tcgaatcaac aaaccatcgg caaagcatgc cgaactaaac
      241 aactttgaat cgcggcctgt ttcaatcagg catacccatg aatgccggac agataagccg
      301 tctccttcgc atttcgatcg aaccatcccg atcgttcggg tcagcgccag gcgctgcccg
      361 cctgcacata aaacgcgttc tgtcccggtc catgcgcgac gtcggtgccc atcgtcacgc
      421 cgagcttgcg agcagtcacg tagcggaagc ccgtgccgca gccgaacgcg gtcggcgcat
      481 ccgaaaacga atgcagacac ccataagcct tgccgacgcc gctaaagcca agcaccgacc
      541 aacgcggcat gacgttccag cgaatctcag cttcggcggt gagcgggttg ttgtcctgga
      601 acttcgcgtc ggacgcgccg cgcagagaca catacggctg cgcgaaaagg cggaatgttt
      661 ccttgcgaga aaccggtgtc ggcgcgcagg ccgagcaccc agcttgtcga cagcgcaatc
      721 cattggtagc cgcgcgcggt ttgttcctga aagttggtgg aactgctcgg cgtcgacggc
      781 aagcggcatg cacaaggaac ggcgaatcac cgaatgcatg ctccgttact ggaacgtggc
      841 gctgatcgac agttgcaccg cattgccctt cggccggttg ctgacgttga actcgttgac
      901 ccaacgcagc gacgccgaga ccggtgtctt ctggaccttg cccgaccagg taaccatcgg
      961 cccgatcccc accgagtggc cctgattgcc gccggtcagt tcggccagtc caccgctgtc
     1021 atgaccgatc tgctggatgt agccgcccac cacgcccacg ccccagccgc tgccaaagcg
     1081 cttgagcgcc agcagatcga gcacgctgac cggcgcgttg tgatagtgcg tgtcattgtt
     1141 gggcgtatag aactcgacgc cgtagttcaa cgacaactcg atctcttctt tcgggaacag
     1201 cttcgtatag gcaaccgtgg gcgtgaaggt ccacgtgttc tggccggcat tggcgagacg
     1261 gctcgagctg taagcaccgg tcggcgcgta aatctgcacg ctgaacgcga cgtgatcggt
     1321 cttggtcagg tgatagccgg ccaccaccgg cgtgaagaag atatcggcga actgggtgtt
     1381 gtgatcgttc ggcaaacggc cgtgaaacga cgagatattg gtgtactgga acggcacccc
     1441 gaacgaggac gcaaaattcc agccgccgag cgtaatgccc cacgtcttga ccgcgttgac
     1501 gatggtgtac accgctgtga cgtcgacgcc cgccgtgatc tggccggcga tcggaatcgt
     1561 cttgctggcg ccgagcgagc cttcgtaata gatcgttgcg gccgaaacga tccagtcgtc
     1621 cgtgggcggc accacaccgg catacggcgt gacctgctgg ccggtgatcg gtctgccgat
     1681 gccaccttca gtcgcccagc cctgctggga aatgccaacg cctgcggcgc tcagaagcgc
     1741 acccgtacat aatctcgcga gcgaccggct ggcggacaaa cggtttgggc gactcatagt
     1801 catcctcatt aattttagat ttcgcattct cacgctgcgg aacaagctgt ggacgaaccg
     1861 cggccgcagg ccgcaggccg cacgcatgta aaacagcgcg ttcctcacgg ttcgcgcctg
     1921 attggcggcg gcgtccagct tccgtcgagt gccggctgct gcggccaata ggcgcgcacg
     1981 tataacgaga acgcttcatt ggccggcgcc ggcagccagt tggcgagttt gtcgtcggct
     2041 gggctcgtgc tctgcacgta gagcgtcaac gagccgtcgg cgttgaactt caggttcttg
     2101 ttcttggtac cgagcgagta gcggttctgc gcattcggcg agaaaaaatg atgcgagttg
     2161 tatagggtca gcgaccagaa gcctttgacc ggcggcaaac tatctttggc aaacgtcacc
     2221 gtgtagtacg cgctgccatc gaggcgctgc cctgacgcgt cggcgtcggc gtagaaatac
     2281 ttcgtctcat tcggcttgtt gacgaagatg ttcgacttcg ccaccgccgt gcgcgtgaag
     2341 tagtccgtgc cgaatgcggc gccgttgctg atggtgttcc agttagcggg cagccgcttg
     2401 ccgaaggtat tgaagttgaa cagcggcgtg accagatccg cctcggcctt ggccgcttcg
     2461 tcgacgactg cggtcttgat cgccggatct ttttgcgccg ccgcgatcaa cgcgcgcgcc
     2521 tgtgcataca gcgcttcttc gccgcgctgc ggcggcgttt cgtcgagcac cacaggcaat
     2581 tcgtcccaga aacgatttgg atcgacccac ttgatctctt cggcgttgcc gcttgcggca
     2641 ccaccggccg gaccgaaact gggcagcttg gaccagtcgg tttccttgac cgtgccgtcg
     2701 tactccgcga gcgggtacag cgcgatcttt gcaatcaccg attgcacggc cacccgatct
     2761 tcaggcgtgt cgtccatgaa aacacgcgga atgaagctgc cgacaccggt cgacgagcgg
     2821 tacacctggc gaatgccctt cggcacctga ccgtgccagt cgggaccggc caacagatag
     2881 aaacccggcc gtgtgccgta catcttgccg agactcgcga agccgtccgt gcgcaggtcg
     2941 acggcctgat acacccagaa ccgtttgccg aaatcgggca cctgcacgac cactggcgtc
     3001 cggttgagat cgaggatcgc ggagccatag accacatcct gattcggaca cgcgacaaaa
     3061 cgctcctgcg gcgcgatgta gtcgcgcagc atcgacagat gattgggcgg cgcgaccgac
     3121 acaacgccgc cgttcagacc cggctgcggc acctgtttca tcgccagcag gcggccgtag
     3181 acattcacca tcggccatgc ccagaagtag gtctggcgcg ccaccagtcg cgcgtattct
     3241 tcggagaccg cgtagccggt caccgggcca ggcacggacg ccgtagtggc cgtcgcatgg
     3301 accggctggt cctgctgcgt cttcgcgaga atcggcgcgg cgaccgacgc cagcacggca
     3361 gccgtgatcc atgtcgcggt tttcttgttc atatcgttct cggcttcgtc catgcgcggc
     3421 ttagcgcttt tgcagttcgg gaaatttcca ggcgccgtcg agcacgcgct ggtccggacc
     3481 atagatccgc atgacgaggt cgtacgcacc gatgggtgcc ggcaaccagt tcgacgcctt
     3541 gtccgcgccc ggcgacgtgt tctgcacgta gatatccagc gagccgtccg cgccgtactt
     3601 caggcctttg gtgcgatcgc cgatcgaata gcgattgatc ggattggcga ccagatgacg
     3661 cgcgggcagg tcgtacagcg tcatcgacca gaagaacttc gcgggcggca ggtccttctt
     3721 gtcgaagtgc aacacgtacg gctgcgcgcc gagcagttgc gcgtgttcgc cgttcacgcg
     3781 cgtgccgacg tagaccgctt cttccttggt gttgccgtaa attcccatcg ccgccgcaac
     3841 ggcgcgcgtg gtgtagtcgt ctttcaggtc cgcgcgactg ccgaacaggt tgaatgaact
     3901 ggtcgtgtgt ttttccgcat cggccaaagc ggctttgccg ccggcgacac ctgcttcaag
     3961 tgcggcgcga atttccggcg gcaacgcggc cggatcgaat ggcgcacccg gcgcaatgcc
     4021 gatcttcgaa aagcgcgcca tcaattccct ctccgacggc gcggacggtt gcgtgaactg
     4081 caagaggaag ttcaggtagg cgatgaagtc gaccgacgtc gccttcgctt catcccattt
     4141 cggaaaatcg agtttcgcga cgggcggcgg tgcagccgta cgctcg
//
LOCUS       DATASH010000744         1763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3288661,
            whole genome shotgun sequence.
ACCESSION   DATASH010000744 DATASH010000000
VERSION     DATASH010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3288661"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VHV99_19840"
     CDS             complement(<1..544)
                     /locus_tag="VHV99_19840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012364307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HEX3637692.1"
                     /translation="MSAELLTSRPTESESTLVLTLSNPGARNALHPDMYAAGIEALDS
                     VERDPSIRAVVITGADNFFCAGGNLNRLLENRAKDPSVQAESIDLLGEWISALRLSSK
                     PVIAAVDGAAAGAGFSLALACDLIVAAEDARFVMSYARVGLTPDGGGSWFLAQALPRQ
                     LATEVLIEGKPIGATRLHELG"
     gene            complement(541..>1763)
                     /locus_tag="VHV99_19845"
     CDS             complement(541..>1763)
                     /locus_tag="VHV99_19845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEX3637693.1"
                     /translation="VSGRCVLASNTSSLSLTAIAAGCSDPSRVVGYHFFNPVPLMKVV
                     EVVDGLRSDRQAGDALMDLARRMGHTPVRAKDMPGFIVNHAGRGMNTEGLRVAGEGVA
                     SFADIDRIMREQAGFRLGPFELLDLTALDVSHPVMESIYHQFYEEPRFTPSPITGTRL
                     AGGLIGRKTGEGFYRYEDGKQHVPAEAPAPTELPPSVWVSKRYPQAHEAVVQLVTKAG
                     VKLDEGATPAAHSLIVVTPFGHDATSAAVDEALDATRVVAIDALFPLLAAQRRTLMTT
                     PATRRAARETAHALFAADGIPVTVIRDSTGFVAQRVVATIVNIGCDIAQKQIATPDDI
                     DLAVMLGLGYPRGPLALGDALGATTILTILRNIFSVLGDPRYRPSPWLARRAQLGLSL
                     TQRDAADTQTENQQ"
BASE COUNT          297 a          554 c          599 g          313 t
ORIGIN      
        1 cgccgagttc gtgcagacgc gtggcgccga tcggtttgcc ttcgatcagc acctcggttg
       61 ccaactggcg tggcaacgcc tgtgcgagaa accatgagcc gccgccatcg ggcgtcagac
      121 cgacgcgcgc atacgacatc acgaacctgg cgtcttccgc ggcgacgatc agatcgcacg
      181 cgagcgccaa cgaaaatccc gcgccggccg ctgcgccgtc aacagcggcg atcaccggtt
      241 ttgacgacag acgcagcgcc gaaatccact cgcccagcag atcgatgctc tcggcttgca
      301 ccgatggatc tttcgcacgg ttttccagca gacggttcag attgccgccc gcacagaaga
      361 agttgtccgc gccggtaatc acgaccgcgc ggatcgacgg atcgcgctcc accgaatcga
      421 gcgcctcgat gcccgcggca tacatgtccg gatgcagcgc attgcgtgcg ccggggttcg
      481 acagcgtgag gaccagtgtc gattcgcttt cggtcgggcg cgaggttagc agttcggcgc
      541 tcattgttgg ttctccgttt gagtgtcggc agcgtcgcgt tgcgtgagcg acaggccaag
      601 ttgcgcacgc cgtgccagcc acggcgacgg acggtagcgc ggatcgccga gcacgctgaa
      661 gatattgcgc aagatcgtga ggatcgttgt cgcgccaagt gcatcgccga gtgcgagcgg
      721 cccgcgcggg tagccgaggc cgagcatcac ggcgaggtcg atatcgtccg gtgtagcgat
      781 ctgcttttgt gcgatgtcac agccgatatt gacgatggtc gccaccacgc gttgcgcgac
      841 gaagccggtc gagtcgcgga tcacggtgac gggaatgccg tcggcggcaa acagcgcgtg
      901 tgcggtctcg cgtgcggcgc ggcgggtggc gggtgtcgtc atcagcgtgc ggcgctgtgc
      961 cgcgagcagc ggaaatagtg cgtcgatagc gacgacgcgg gtcgcatcga gtgcttcgtc
     1021 gacggcagcg ctggtcgcgt catgaccgaa cggtgtcacg acgatcagcg agtgcgctgc
     1081 gggcgtggcg ccttcgtcga gctttacccc ggccttggtg acgagttgca cgacggcttc
     1141 gtgcgcttgc ggatagcgct tgctgaccca cacgctcggc ggcagttcgg tcggcgcggg
     1201 cgcctcggcg ggcacgtgct gtttgccgtc ttcatagcgg tagaagcctt cgcccgtctt
     1261 gcggccgatc aatccgcccg cgagcctcgt gcccgtgatc ggcgaaggcg tgaagcgcgg
     1321 ctcttcgtag aactggtgat agatcgattc catcacggga tgcgatacgt cgagcgcggt
     1381 cagatcgagt agttcgaacg gcccgagacg aaaccccgcc tgctcgcgca tgatgcggtc
     1441 gatgtcggcg aagctcgcca cgccttcgcc cgccacacgc aggccttctg tgttcatccc
     1501 gcggccggca tgattgacga tgaagccggg catgtctttc gcgcgcaccg gcgtgtggcc
     1561 catgcggcgc gcgaggtcca tcaatgcgtc gcccgcctgg cggtcgctgc gcaggccgtc
     1621 caccacttcg acgactttca tcaacgggac cggattgaag aagtgatagc cgactacacg
     1681 cgatgggtcg ctgcagccgg ccgcgattgc ggtgagggac agcgacgacg tgttggaagc
     1741 gagcacgcaa cggccgctca cca
//
LOCUS       DATASH010000745        14306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1903863,
            whole genome shotgun sequence.
ACCESSION   DATASH010000745 DATASH010000000
VERSION     DATASH010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 14306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14306
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1903863"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1418..2932)
                     /locus_tag="VHV99_19850"
     CDS             complement(1418..2932)
                     /locus_tag="VHV99_19850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEX3637694.1"
                     /translation="MVNFAFPRHLRLTFPGAAALTLAACSFGPNGAPPAMPQPAHYGA
                     EAQPTQTVPAQGITQQFVVGAKPVPEWWKLYQSDALNALVDDGLRNSPTLAATDKSLA
                     AAREQLRAQIGSSLLPTIDAGGQMTRNRALAIPEIGPNTFLYNVFVGQLQAHYTFDLF
                     GAARLADAALAARVNVQAYQFDAARRALAANIVTAAITSAALHAQIDTTERLVTIAND
                     QARDTQRRYTLGAVSHTDQLNAQQSAASLSASLPGLKQQWLTTRHALAVLLGRTPDAA
                     PDDLDLAQLHVPEQVPVVVPSELLRTRPDIQAADAALKAAAADVGVATAQMFPSLSLS
                     ASMGQGGFSWPVALSGAGAIWSIGASLSQPLFHGGALFAQRRAAMDTYDATVSQYKQT
                     VLAAFQNVADTLAALQHDTQALDAANIAARSAQGIFDETSGRYRLGALPIASTRSSEQ
                     QFRNAQLDEIRYTSARLTDTAALFQAMGNPPVESEPSAAASTAAVPATNVGVSD"
     gene            complement(2937..6065)
                     /locus_tag="VHV99_19855"
     CDS             complement(2937..6065)
                     /locus_tag="VHV99_19855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX3637695.1"
                     /translation="MSTTHEEGRFNLSAWALRHQALVVFLIALATVFGILAYTRLAQS
                     EDPPFTFRVMVIRTFWPGATARQVQEQVTDRIGRKLQETPAIDFVRSYSRPGESLMFF
                     SMKDSAPVKDVPETWYQVRKKVGDIAATLPQGIQGPFFNDEFGDVYTNIWTLEGDGFS
                     TAQLHDYADQLRTVLLRVPGVGKVDYFGDPDQHIYIEIANTQLTRLGISPQQLGQAIN
                     SQNSVAPAGTLTTTDDRVFVRPTGQLQDVNALADTLIRINNRSFRLGDIATIKRGYDD
                     PVATQMRSGGKAVLGIGVTMQPGGDVIRLGKALDEETVNLRAYLPAGLHLVEVSSMPH
                     AVALSVDDFLEAVAEAIAIVLVVSLLSLGVRTGMVVVISIPVVLAVTALCMYLFDIGL
                     HKVSLGTLVLALGLLVDDAIIAVEMMSVKLEQGWSRTRAAAFAYTSTAFPMLTGTLVT
                     VAGFLPIALAKSSTGEYTRSIFEVSAIALIASWLAAVVLIPLLGYHMLPERKRQAHEA
                     DDHEHDIYDTRFYKRLRGWIGWCIERRFVVLAITVVLFILAMAGFTLVPQQFFPSSDR
                     PELLVDVRLPEGASFDATLRQAERLEKVLVGRPEIDHTVNFVGTGAPRFYLPLDQQLP
                     QPNFAQFVITAKSVKDREKLAQWLEPELRNNFPAIRTRLSRLENGPPVGYPVQFRVSG
                     DDIATVRSIAERVAATMRANRGTRNVQFDWDEPAERSVRFEVDQTKARELGVSSEDIS
                     SFLAMTLSGYTVTQYRERDKLISVDLRAPKAERVDPAHLVTLAMPTPNGPVPLGTLGH
                     LRNDLEYGVIWERDRQPTITVQSDVAPGAQGIDVTHAVDKALSQIRATLPVGYRIEIG
                     GSVEQSGKGQTSINAQMPIMIIAVLTLLMIQLQSFARVLMVVLTAPLGLIGVVLTLLL
                     FGQPFGFVAMLGVIAMFGIIMRNSVILVDQIEQDIAAGHKRFDAIVGAAVRRFRPITL
                     TAAAAVLALIPLLRSNFFGPMATALMGGITSATILTLFYLPALYAMSFRVRADEREPQ
                     TPSESGTTHTGN"
     gene            complement(6062..7288)
                     /locus_tag="VHV99_19860"
     CDS             complement(6062..7288)
                     /locus_tag="VHV99_19860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX3637696.1"
                     /translation="MRQQFRRYARPARLRRAAFALALAGIVTLAACSKKEPAAPAPRP
                     VVAVAVHADGSATAAASLPGEVQARYSTPLSFRVSGKIIERRVRLGDVVKNGQILARL
                     DPADAQKNAAGAQAQLEAAQHSFVYAKQQLDRDQAQARENLIAPAQLEQTTNAYASAA
                     AQRDQATQQAALSKDQLQYTTLIADHAGVITAEQADTGQNVSAGQAVYNLAWSGDIDV
                     ICDVPEGALARLSVRQTAKVTLAALPGRSFNARVRELSPAADPQSRTYRAKLTLDNPG
                     PDVRLGMTADIALAAPSPSNSVANPAANSAASQSSAFTVPATALFHDGTQPAVWVVRP
                     MDNAPGVGTSGVGTSGIGTLELRRVTVTRYNERTIVIGQGLKDGERVVLQGVHTVTAG
                     EKVHVIPPLHPEDFAS"
     gene            7508..8125
                     /locus_tag="VHV99_19865"
     CDS             7508..8125
                     /locus_tag="VHV99_19865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX3637697.1"
                     /translation="MKRQRLTREQSKDQTRQRLLDAAQTIFMKKGFVAASVEDIAGAA
                     GYTRGAFYSNFRSKSELFLELLRRDHETMQAGLQAIFENAAPREEMEARVLRYYSSLH
                     RENKCFLLWVEAKLLAVRDGRFRLRFNAFMHEKHEQLSAYIHEFSTRVGTPLLLPAET
                     LAMGLMGLCDGVQFFYTVDPQNVPAEMAESVLAGFFARVVFGRDA"
     gene            complement(8142..8354)
                     /locus_tag="VHV99_19870"
     CDS             complement(8142..8354)
                     /locus_tag="VHV99_19870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637698.1"
                     /translation="MPHRYQLFEKIAFSLVCWSAAACSAFIAYERCPDIKVILHVGEE
                     ALRYTGQYSFVCASPVADACAQLPTD"
     gene            8609..9655
                     /locus_tag="VHV99_19875"
     CDS             8609..9655
                     /locus_tag="VHV99_19875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017334637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3637699.1"
                     /translation="MNQLQSMRVFVKVADLGSFVRAASTLELSTAVVTRHVADLEARL
                     GTRLLNRTTRRLSLTESGTTYLERVRHILDDLEGVEQMVVARNHEPVGTLRIVAPVVF
                     GLHSLAPVVQSYAACYPDVRPDVTLADRHVDLVEEGFDVGIMVARQMRSASIVTRRLT
                     TGYMTVCATPAYLAQHGTPTRPEDLLMHPCLSRPAEQGGDERVFTGPKGEVRVRPTNA
                     IAANNTEMLRQFALLGMGVAILPSYLIGRDLASGRLVPLLSDYRLPQIEITIAYPSRL
                     HLPAKVRTFIDHLVEHFSQTDAQARDPRATMPRRAAAASAVYACDLPVSASPGEAARP
                     KRQASRSGIAVSSL"
     gene            complement(9693..10082)
                     /locus_tag="VHV99_19880"
     CDS             complement(9693..10082)
                     /locus_tag="VHV99_19880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX3637700.1"
                     /translation="MSNHQTIQRPQRAINFAEKLSLFSDQWQARVIAEMNDYQFKLVR
                     IEGDFIWHEHTDTDETFIVLDGQLRIDMREGSVLLSAGEMFVVPKGTQHKPYAEREVK
                     LLLIEPRGVKNTGEESNERTAENDLWI"
     gene            complement(10209..11093)
                     /locus_tag="VHV99_19885"
     CDS             complement(10209..11093)
                     /locus_tag="VHV99_19885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX3637701.1"
                     /translation="MSAKQVLAVSGISSHLKPSADWIHRSPHASGTSGIERIEAFFHH
                     AGYAMHRHDTYAIGHTLAGVQSFRYRGSMRNSLPGGTMVLHPDEPHDGQAGTRDGFHY
                     RMIYVEPALFQQVLGGKPLPFVEGGLSNDPRLFAATERLLRTMDCAIDSLEQDDALYD
                     LAIALDAVAGAAATRRPVDYLSAERAREHMLASLDRAVTLDELAAAAGRDRWSLSRDF
                     RALFGTSPHRYLTMRRLDLVRRLAVQGVSLADAAAAAGFTDQSHMTRHFKRAWGVAPA
                     HWLKMLGPSTRGSARRPL"
     gene            complement(11090..11329)
                     /locus_tag="VHV99_19890"
     CDS             complement(11090..11329)
                     /locus_tag="VHV99_19890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HEX3637702.1"
                     /translation="MTASAIPGIPGESIDLQIADVLREVRFPTYKDALVDAAREAGAS
                     NEVLSMLDGLPEQDYADVNSVTRLVGGNYGPGLGA"
     gene            complement(11354..11608)
                     /locus_tag="VHV99_19895"
     CDS             complement(11354..11608)
                     /locus_tag="VHV99_19895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012400657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637703.1"
                     /translation="MKTSTLLTMVTLSASCFAAPVHTAPDGTAANSLAERANAAPVAP
                     AAPDVAATDDNRPQQWQHIALPKSRHLERSFTGQNEHLQT"
     gene            complement(11864..13201)
                     /locus_tag="VHV99_19900"
     CDS             complement(11864..13201)
                     /locus_tag="VHV99_19900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HEX3637704.1"
                     /translation="MSEPSLDAVRRDQAGWIFLHIEGEPYDRGEQHGQLLATEIRNAI
                     HTARYLAKWDTGEDFDTFVDAATSQFANKLDTEFADEIQGIADGAKLPFAEVLAWNGY
                     MDLLQSWWPSHAAARQPQLGLKPWRGRRGHHCSAFIATGKATRDGRIVMAHNSWDRYA
                     AGDAFNVVFDIVPDRGKRILMQGLPGCISSLTDFWVTEAGLIVTETTISNFVGYNAAG
                     APEFYRSRRASQYANDIGEWCEMFAVANNGGYANSWLLGDTKTGEIARYELGLHFSGF
                     ESTKDGFYSGYNTATDLKIRNQECIGEGEDYTDVRKNGARRLRFMQLEEMHHGKIDVE
                     LAKEMIADHHDVYLDRNDNPCSRTICGHLELDDARFGGTDHGPFNPWGANDGKVVDSE
                     MARAMAFDARWGHPCGRPFDAQAFMKRHPQWNWLNGYMRDRPSWPWTRFEMLR"
     gene            complement(13443..>14306)
                     /locus_tag="VHV99_19905"
     CDS             complement(13443..>14306)
                     /locus_tag="VHV99_19905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClcB-like voltage-gated chloride channel
                     protein"
                     /protein_id="HEX3637705.1"
                     /translation="WTPEVWGNGYSVVNSILHSPWTWTALVLVLVFKIVATSATVGSG
                     AIGGVFTPTLFVGAVVGCLFGQGMHTLWPHGTSAPFAYAMVGMGAFLAGATQAPLMAI
                     LLIFEMTLSYQVVLPLMLSCVVAYFVSRAIGKTSMYEVTLRRNQEEQARTRLRATQMR
                     ELIRPAETVVPPNASVQDMTRVFLEYPVKYLYVANASGAFLGVVALKDITSDLLDKRE
                     TATKTAADYLQPHFDVLTPDMPLGVALQHFMAFQGERLPVVESTADPKLAGVVYKTSL
                     LDAYFRMNPTR"
BASE COUNT         2630 a         4445 c         4574 g         2657 t
ORIGIN      
        1 gagttgtttg tctgctgggc gattttcggc tgtggtttta tggcgtgggc ctttccttga
       61 attgttggtg gtctattagc gttgcccctg tgcggggcgg cacctacttt tctttgcctg
      121 ccgcaaagaa aagtaggcaa aagaaagcgg ctcacaccgc cagcccataa gcgggtccct
      181 cgcgcaggtg cggtagtggt gcatgtggaa tctgtgttct cgcacatttg gcgttagtga
      241 cacagcagtc atacttccgg cgacgctgcg cgcgccgaag agcacggcat aaaaccgccc
      301 gctacttctt aactctcgcg ttccgcccaa taccgttccc cgtcattgct ttcccaccgc
      361 ctttctggct ccgactccga ctccgactct ggctctgact ctgactctga ctctgactct
      421 gactctgact ctgactctga ctctgacgat ggctctagct ctagctccag gtcgggatct
      481 ggctgcgagt gcgacatgtc gacatttcat tcgcggtggg gttcactgat gagtgtgggg
      541 gagcatggcc gaggcgaagc cgatggcttc cactgcgccg tgacgaagct cctggtttcc
      601 caggcagacc catccgcgac gcacgcggtg cggagtggga gctgatgagc gcgttgtcgc
      661 taaggttgaa tgtgcgaggg cacggattcc agatgtacca ctaccgtgac tgtgcgaggg
      721 acccgcttat gggctggcgg ggtgagccgc tttctttgct tatctttctt tgcggcggca
      781 aagaaagtaa gtgccgcccc gcacaggggc gacgctaata gaccaatatc aattcaagga
      841 aaggccaacg ccacggacaa acaaccaaac ccaccgccgc aggcaaacaa ttcaaggaaa
      901 agccaaaaag ccagatcaag gaaagcccaa cgccacaggc aaacagacaa tcaaccgccg
      961 cgcgggaaaa acccaatcca cgtgctacgc gcaaaaccct cgatctctca atcacttacg
     1021 cggaatgccc agacgttatc aacaagccta taaacagaat ctgtggataa gacgcccccc
     1081 aaaatacgcc cgtcctcacg aggcgcgttg tcgatcaacc ctgcatccag tcgcaccgcg
     1141 cgcaacaaac tgatctgcat caagcactta agcgcgtagc cccacagtta tcaacaaccc
     1201 tgtcaacata aattggggat aacgttgagt tatgttgagg cattcccaca ccctctaccg
     1261 aaaacagaag gcaccttcag cacccctggt tcattccgca ccgcgagcag aaaaccccgc
     1321 gcgctcaacg tgttagcccg cttgtcagat agttttcaac agcctcatca acataaactg
     1381 gggataagtc cgtaaccatc aatacggaat cccaggttta gtcagaaacg cccacgttcg
     1441 tcgcaggaac tgccgccgtc gacgccgccg cagacggctc cgattccacc ggtggattcc
     1501 ccatcgcctg gaacaaggcc gcggtgtccg tcagccgcgc gctcgtatag cgaatctcat
     1561 caagctgcgc attacgaaac tgctgttcgc tcgaacgcgt cgacgcgatc ggcaacgcgc
     1621 ccagccggta gcgtcccgac gtctcgtcga aaattccttg cgccgagcgc gcggcaatgt
     1681 tcgccgcgtc gagcgcttgc gtgtcgtgct gcaacgccgc aagcgtgtcg gcgacgttct
     1741 ggaatgcggc cagcacggtt tgcttgtact gcgaaaccgt ggcgtcatac gtgtccatcg
     1801 ccgcgcggcg ctgcgcgaac agcgcgccgc catgaaacag cggttgtgac aaagacgcgc
     1861 caatactcca tatcgccccc gcgccggaca gcgccaccgg ccagctaaag ccgccttgtc
     1921 ccatcgatgc actcagcgaa agactcggga acatctgcgc ggtcgccacg ccgacatcgg
     1981 ccgcggcggc tttcagcgcg gcgtccgcgg cttgaatgtc gggccgggtg cgcagcaatt
     2041 cggatggcac cacgaccggc acctgctcag gcacatgcag ttgcgcgaga tcgaggtcgt
     2101 ccggcgccgc gtccggcgtg cggccgagca gcactgccag agcatgacgc gtcgtcagcc
     2161 attgctgctt caaacccggc aaactcgcgg acagactcgc cgcactctgt tgcgcgttca
     2221 actgatcggt gtgcgagacc gcgcccaacg tgtagcgccg ttgcgtgtcg cgcgcctgat
     2281 cgttggcaat cgtcacaagc cgctcggtcg tgtcgatctg cgcatgcaac gccgcgctcg
     2341 taatcgccgc cgtcacgatg ttggcggcca gcgcgcggcg tgcggcatcg aactgatagg
     2401 cctgcacatt cacgcgagca gcgagcgccg catcggcgag acgtgcggcg ccgaacaggt
     2461 cgaacgtata gtgcgcttgc aactgaccga cgaagacgtt gtacagaaac gtgttcggtc
     2521 cgatctcggg tatcgccagc gcgcggttgc gggtcatctg accaccggcg tcgatggtcg
     2581 gcaacagcga actgccgatc tgcgcccgca attgctcgcg tgcggcggca aggctcttat
     2641 cggtggcggc gagtgtcggg ctgttgcgca ggccgtcgtc gaccagcgcg ttcagcgcat
     2701 ccgattgata cagcttccac cattcgggca cgggcttggc gccaaccacg aattgctggg
     2761 tgatgccctg tgcgggcacg gtttgggtcg gctgcgcctc ggcgccgtag tgagccggtt
     2821 gaggcatggc gggtggcgcg ccgttcgggc cgaacgagca ggcggccagc gtcagcgcgg
     2881 ccgcgccggg aaacgtgagg cgcaggtgtc gagggaaggc aaagttaacc atgatctcaa
     2941 ttccccgtgt gcgtcgtgcc ggactcggac ggcgtctgcg gttcgcgttc gtcggcgcgc
     3001 actctgaacg acatggcgta cagggccggc agatagaaca acgtgaggat ggtcgcgctg
     3061 gtaatgccgc ccattagcgc ggtcgccatc gggccgaaga agttcgagcg caacagtgga
     3121 atcagcgcga gcacggccgc tgccgcggtc agcgtgatcg gccggaagcg tcgcaccgcc
     3181 gcgccgacga tcgcgtcgaa gcgcttatgc cccgcagcga tgtcctgctc gatctgatcg
     3241 accagaatta ccgagttgcg catgatgatg ccgaacatcg cgatcacccc gagcatcgcg
     3301 acaaagccga acggctggcc gaacagcagc agcgtcaaca ccacgccgat caggccgagc
     3361 ggcgcggtca gcaccaccat cagcacgcgc gcgaaacttt gcagctggat catcagcagc
     3421 gtcagtacgg caatgatcat gatcggcatc tgcgcgttga tcgatgtttg acccttgccg
     3481 ctttgctcga ccgagccgcc gatttcgatc cggtagccga ccggcagcgt ggcgcgaatc
     3541 tggctcaagg ctttgtcgac ggcatgcgtg acgtcgatcc cttgcgcgcc gggcgcgacg
     3601 tcggattgca cggtgatagt gggttgccga tcgcgttccc agatcacacc gtattcgagg
     3661 tcgttgcgca gatgaccgag cgtgccgagc ggaaccggcc cgttcggcgt cggcatggcc
     3721 aacgtgacga ggtgcgcggg gtcgacgcgt tcggctttgg gcgcgcgcag atcgacgctg
     3781 atcagcttgt cgcgctcgcg atactgggtg acggtgtagc cggagagtgt catcgcgagg
     3841 aagctcgaga tgtcttcgga gctcacgccg agttcgcgcg ccttcgtctg gtcgacttcg
     3901 aagcgcaccg aacgctcggc gggctcatcc cagtcgaact ggacgttgcg ggtgccgcgg
     3961 ttggcgcgca tggttgcggc cacgcgctcg gcgatcgaac gcacagtggc gatatcgtcg
     4021 ccgctgacgc ggaactgcac gggatagccg accggcgggc cattctccag acgcgacagg
     4081 cgggtgcgga tcgccggaaa gttgttgcgc aattccggtt cgagccattg cgcgagcttc
     4141 tcgcgatcct tcaccgattt cgccgtgatg acgaactgcg cgaaattggg ctgcggcaat
     4201 tgctggtcga gcggcagata gaaccgcggc gcgccggtgc cgacgaagtt caccgtatga
     4261 tcgatttccg gacggcccac gagtactttc tccaaccgct ctgcttgccg caacgtcgcg
     4321 tcgaacgagg cgccttcggg caagcgcacg tccaccagca actcgggacg gtccgagctc
     4381 gggaagaact gctgcggcac cagcgtgaag ccagccatgg ccagaatgaa cagcacgacg
     4441 gtgatcgcca gtacgacgaa acgccgctcg atgcaccacc cgatccagcc acgcaagcgc
     4501 ttgtaaaaac gggtgtcgta gatgtcgtgt tcgtgatcgt ccgcttcgtg tgcctgacgt
     4561 ttgcgctccg gcagcatgtg atagccgagc aacggaatca gcaccacggc cgcgagccac
     4621 gacgcgatca gcgcgatcgc cgacacttcg aagatcgagc gcgtgtattc gccggtgctc
     4681 gacttcgcca acgcaatcgg caggaagccg gccacggtca cgagtgtccc ggtcagcatc
     4741 ggaaaagcgg tactggtgta cgcgaaggcg gctgcgcgcg tgcggctcca gccttgttcg
     4801 agtttcaccg acatcatttc gacggcgatg atcgcgtcgt ccaccagcag ccctaaagcc
     4861 agcacgagcg tgcccaacga caccttgtgc aggccgatat cgaacaggta catgcacagc
     4921 gccgtgacgg cgagcacgac aggtatcgag atcacgacca ccatgccggt gcgcacgcct
     4981 agcgacagca ggctgaccac cagcacgatc gcaatggcct cggcgacggc ctcgagaaaa
     5041 tcgtcgactg aaagcgccac tgcgtgcggc atgctcgaca cttcgaccag gtgcaaaccg
     5101 gcgggcaggt aggcacgcag gttgaccgtc tcctcgtcga gcgccttgcc caggcgaatc
     5161 acgtcgccgc cgggctgcat cgtgacgccg atgccgagca ccgccttgcc gccggagcgc
     5221 atttgcgtgg cgaccgggtc gtcgtaaccg cgcttgatgg tcgcgatgtc gccgagacgg
     5281 aacgagcggt tgttgatgcg gatcagcgta tcggcgagcg cattgacgtc ctgcaactga
     5341 ccggtggggc gcacgaacac gcggtcgtcg gtggtggtga gcgtacccgc cggcgcgacg
     5401 ctgttctgcg aattgatcgc ctggccgagc tgctgcggcg agatgccgag gcgcgtgagt
     5461 tgcgtgttgg cgatctcgat gtagatgtgc tgatccggat cgccgaaata gtcgaccttg
     5521 ccgacgcccg gcacgcgcag cagcacggtg cgcaactggt ccgcgtaatc gtgcagttgc
     5581 gcggtggaaa agccgtcgcc ttcgagcgtc cagatattgg tgtagacgtc gccgaattcg
     5641 tcgttgaaga acgggccttg aatgccttgc ggcaaggtcg ccgcgatgtc gccgactttc
     5701 ttgcgcacct gataccaggt ttcgggtacg tccttgaccg gcgccgagtc tttcatcgaa
     5761 aagaacatca gcgattcgcc ggggcgcgag taactgcgca cgaaatcgat ggcgggcgtt
     5821 tcctgcagtt tccggccgat gcggtcggtg acctgttcct gtacctggcg cgcggtcgcg
     5881 ccgggccaga aggtgcggat caccatcacg cggaacgtga agggcgggtc ttcagattgg
     5941 gcaagacggg tataggcgag gatgccgaat acagtggcga gcgcgatcag gaaaacgacc
     6001 agcgcctggt gccgcagcgc ccatgcggac aggttgaagc gtccttcttc atgcgtggtg
     6061 ctcatgaagc gaagtcctcg ggatgcagcg gcgggatcac gtggactttt tcgccggcgg
     6121 tcacggtatg cacgccttgc agcacgacgc gttcaccgtc cttgagcccc tggccgatca
     6181 cgatcgtgcg ctcgttatag cgcgtgaccg tcacgcggcg cagttccagc gtaccgatgc
     6241 ccgacgtgcc gacacccgac gtaccgacac ccggcgcatt atccatagga cgcacgaccc
     6301 acacggccgg ctgcgtgccg tcatgaaaca gcgcggtggc cggcacggtg aaggcactgc
     6361 tctggctcgc agctgagttg gccgccgggt tggcaactga gttcgagggc gaaggcgcgg
     6421 cgagcgcgat atcggcggtc atgccgagac gcacgtccgg tccgggattg tcgagcgtga
     6481 gcttggcgcg gtaagtgcgg ctttgcggat cggcggcggg cgacagttcg cgcacacgcg
     6541 cgttgaagct gcggccgggc agcgccgcca gcgtgacctt cgcggtttgc ctgaccgaga
     6601 gcctggccag cgcgccttcc ggcacgtcgc agatgacgtc gatatcgccg ctccatgcga
     6661 ggttgtagac ggcctggccc gcggacacat tctggccggt gtcggcttgt tcggcggtga
     6721 tcacgccggc gtgatcggca atgagcgtgg tgtactgcaa ctggtctttc gacaacgccg
     6781 cctgctgcgt ggcctggtcg cgctgcgcgg cagcggacgc gtaggcattg gtggtctgtt
     6841 cgagctgcgc aggcgcgatc aggttttctc tcgcctgtgc ctggtcgcga tcgagttgct
     6901 gtttcgcata cacgaagctg tgttgggcgg cttcgagttg ggcctgcgcg ccggcagcat
     6961 tcttctgcgc atccgccgga tccagccggg cgagtatctg cccgttcttc accacgtcgc
     7021 ccagacgtac gcgccgctcg atgatcttgc cgctgacgcg aaacgataac ggtgtggaat
     7081 agcgcgcctg tacttcgccg ggcaacgatg ccgcagctgt ggcgctgccg tccgcatgca
     7141 cggcgacggc gaccaccgga cgcggcgcgg gcgcggccgg ttctttcttc gagcaggcgg
     7201 ctagcgtgac gataccggcg agagcgaggg cgaatgctgc gcggcgcaag cgcgcgggac
     7261 gggcataccg acggaactgc tggcgcatcg cagacgatgc gggaggaccg ggacgcttca
     7321 caattacccc aactggagat agcgaacgaa cacggcaagc cgcagcggcc cgccagcgaa
     7381 gacgcacagg ccgggacatg caattggcat acgcgtctga cttcgaatgg acgcttgaat
     7441 tctaatacac acgtgtattt gaatgttgcg gtgaattggg attcttttcg gtgctagact
     7501 tgcgcccatg aaacggcaac gacttacacg cgagcagagc aaggaccaga cgcgtcagcg
     7561 tctgctcgac gctgcgcaaa cgatttttat gaagaaaggc tttgtcgcgg cgagtgtcga
     7621 agacatcgcc ggggcggcgg gctatacgcg cggcgcgttc tattcgaact tccgcagcaa
     7681 gagcgagctg ttcctcgaac tattgcggcg cgatcacgag accatgcagg cggggttgca
     7741 ggccatcttc gaaaacgcgg ccccgcgtga agagatggaa gcgcgcgtgc tgcgctacta
     7801 cagtagcctg catcgcgaaa acaagtgctt cctgctgtgg gtggaggcga agctgctcgc
     7861 cgtgcgcgac ggccgcttcc ggctgcgctt taacgcgttc atgcacgaga agcacgaaca
     7921 gttgagcgcg tacatccacg aattttcgac gcgcgtcggc acgccgctgt tgctgccggc
     7981 ggagacgttg gcgatgggct tgatgggctt gtgcgacggc gtgcagttct tctacacggt
     8041 cgacccgcag aacgtgccgg cggagatggc cgaatcagtg ctggcgggat ttttcgcgcg
     8101 cgtggtgttc gggcgcgacg cctaaggcaa atgccggcga atcagtccgt cggcaactgc
     8161 gcgcaggcgt ccgcaaccgg tgaggcgcag acgaatgaat actgcccggt gtagcgcaga
     8221 gcttcttcac caacgtgcaa gatgaccttg atgtccgggc accgctcgta agcgatgaag
     8281 gccgaacagg ctgcggcgga ccagcaaacc agtgaaaacg cgattttctc gaagagctga
     8341 taacgatgtg gcatagcgat taagttcctt tcgggcgcta tattaccagc atataagggt
     8401 ttatacctag ctattttccc ttggttgact tcgccatagt gtcgcgagcg cctcataaac
     8461 gtgaccggat tgtcatgttc gggtcagggt tgggacatgg tcggatcgat acaatcggat
     8521 cgttgggcag acccacgcgc gccttgccgc tcgggtcgac acaggcgttg ttgcgccggc
     8581 ccggctttac cgaggcagga gtcgccccat gaatcaattg caatcgatgc gcgtgttcgt
     8641 caaagtggcc gatcttggca gttttgtccg cgcggccagc acactggaac tctccaccgc
     8701 tgtcgtcacg cggcatgttg ccgatctgga agcgcgcctc ggcacccgcc tgctcaatcg
     8761 cacgacgcgc cgtctttcgc tgaccgaatc cggcacgact tatctggagc gcgtgcgtca
     8821 tattctcgac gacctcgaag gcgtcgagca gatggtggtg gcacgcaatc acgaaccggt
     8881 cgggaccttg cgtatcgtcg ccccggtagt gttcgggctg catagcctcg ccccggtggt
     8941 gcagtcctac gccgcgtgct atccggatgt caggcctgac gtcacgctgg cggatcgcca
     9001 tgtcgatctg gtggaagagg gtttcgacgt cggcattatg gttgcgcggc agatgcgcag
     9061 tgcgagcatc gtcacgcggc gtctgaccac cggctatatg acggtctgcg cgaccccggc
     9121 ctatctcgcg cagcacggca cgccgacccg cccggaagat ctgttgatgc atccatgtct
     9181 gagccggccc gccgagcaag gtggcgacga acgggtgttc accggaccca agggcgaagt
     9241 ccgcgtccgg ccgaccaacg cgattgcggc gaacaatact gaaatgctga ggcagttcgc
     9301 cctgctgggc atgggcgtgg caattctgcc gagctatctg attggccgcg atctggcgtc
     9361 cgggcgtctc gtgccgttgc tcagcgatta ccgcctgccg caaatcgaaa tcacgattgc
     9421 ttatccgagc cgccttcatt tgccggcgaa agtgcgcacc tttatcgatc atctggtcga
     9481 gcatttcagt cagaccgacg ctcaagcgcg cgatccacgc gccacgatgc cgaggcgtgc
     9541 ggctgccgcc tcggcggttt atgcctgtga cctgccggtg tccgcctcgc ccggcgaggc
     9601 ggcgcggccg aagcgtcagg cttcgcgctc gggaatcgcg gtgtcgtcgc tttgacggga
     9661 gcggggcgta cgcggcgaac gcgtgcgtcc gcctagatcc acaggtcgtt ttcagcggtg
     9721 cgttcgttgc tttcttcgcc ggtgttcttc acgccgcgcg gttcgatcag cagcagcttc
     9781 acctcgcgtt cggcgtacgg cttgtgctgc gtgcctttgg gcaccacgaa catttctcct
     9841 gccgagagca gcacggaccc ctcgcgcatg tcgatgcgca attgaccgtc gagcacgata
     9901 aacgtctcgt ccgtgtcggt atgctcatgc cagataaagt cgccttcgat gcggaccagc
     9961 ttgaactggt agtcgttcat ttccgcgatg acgcgcgctt gccattggtc gctgaagagc
    10021 gacagctttt cggcgaagtt gatggcgcgt tgaggccgtt gaatcgtttg gtggttggac
    10081 attgaggtgc tcctcctgag tggtaggtct caggcaggag cttagggcgg caggcgctat
    10141 cgggtcttgt acgtttgtgc gcgtggcttt cgcaggtcgc gcggcgggct atcaggcagc
    10201 ggtgcccgtc agagcggccg cctcgcgctg ccgcgcgtcg aaggccccag catcttcagc
    10261 caatgcgccg gcgcaacgcc ccaggcgcgc ttgaagtgtc gcgtcatatg actttgatcg
    10321 gtaaagccgg ctgccgccgc tgcgtccgcg agcgacacgc cttgaaccgc cagccgtcgc
    10381 acgagatcga gccgacgcat ggtcagatag cgatgcgggc tcgtgccgaa cagcgcacga
    10441 aaatcccgcg acagactcca gcggtcgcgg ccggcggctg cggccagttc gtccagtgtc
    10501 actgcacgat cgagcgacgc aagcatatgc tcgcgcgccc gttcagccga aaggtaatcc
    10561 accggacgcc gcgtcgctgc cgcgcccgcc acggcgtcga gcgcgattgc cagatcgtaa
    10621 agcgcatcgt cctgttcgag cgaatcgatt gcgcaatcca ttgtgcgcag caggcgctcg
    10681 gtcgcagcaa acagtcgcgg atcgttcgac aggccgcctt cgacgaacgg cagcggcttg
    10741 ccgccgagca cctgctggaa cagtgccggc tcgacataga tcatccggta gtgaaagccg
    10801 tcgcgggtac cggcctggcc gtcatgcggt tcgtcgggat gcagcaccat cgtgccgccc
    10861 ggcaggctgt tacgcatgct gccgcggtaa cggaagctct gcacgcctgc cagggtgtgg
    10921 ccgatcgcat aggtatcgtg ccggtgcatg gcataaccgg cgtgatgaaa gaacgcttcg
    10981 attcgttcga tgccgctcgt gcccgacgca tgcggcgagc ggtggatcca gtcggcagat
    11041 ggcttcagat gcgacgaaat cccgctcacc gccaagacct gctttgcgct catgccccaa
    11101 ggcccggccc gtaattgcca ccgacgaggc gcgtgaccga gttcacgtcg gcgtaatcct
    11161 gttccggcaa accgtcgagc atcgacaata cctcgttgct cgcgccggct tcgcgcgcgg
    11221 cgtcgaccag cgcgtccttg taagtcggga aacggacttc gcgcagaacg tcggcgatct
    11281 gcagatcaat cgattcgccg ggaatgcctg gaatagccga tgcggtcatg cgcgcgctcc
    11341 tatgggtgtt ggttcaggtt tgcaagtgct cgttctggcc cgtgaagctt cgctccagat
    11401 ggcgcgactt gggcagcgcg atgtgttgcc attgttgcgg acgattgtcg tccgtggcag
    11461 cgacgtccgg agccgcgggg gcgacaggtg cggcgttggc gcgttcggca aggctattgg
    11521 cggccgtgcc gtccggcgcg gtgtgaaccg gcgcggcgaa acacgaagct gacagcgtca
    11581 ccatggtcaa cagtgtcgag gttttcatcg cccgacctcc taagctcatg cggcgctgac
    11641 ggttgagcaa atgctgtgcc gtccccgcgt cgaaaggagg gcgcaacctg cacgcgggtt
    11701 gcttgcggcc gacatgcttc taccatctta gatggcaaac cgcggcgccg cgcgcgcaaa
    11761 agagacaaaa cctaattgca gatttcacat gatggcagga aaaagccgag gcggtcgcga
    11821 acggtcgccc cagcgccgcc ggccaccgag ggaaagcccc tctctaacgc agcatctcga
    11881 accgcgtcca cggccacgac ggccggtcgc gcatatagcc gttgagccag ttccactgcg
    11941 gatggcgctt catgaacgcc tgggcatcga acggccggcc gcacggatgc ccccagcgcg
    12001 catcgaaggc catggcgcgc gccatctcgc tgtcgaccac cttgccgtcg ttggcgcccc
    12061 acggattgaa cggcccatgg tcggtgccgc cgaagcgcgc gtcgtcgagt tccaggtgcc
    12121 cgcagatggt gcgcgagcac gggttgtcat tgcggtcgag gtagacgtcg tgatgatcgg
    12181 cgatcatctc tttggcaagc tcgacgtcga tcttgccgtg atgcatctct tcgagctgca
    12241 tgaagcgcaa gcgacgcgcg ccatttttgc gcacgtccgt atagtcttcg ccctcgccaa
    12301 tgcattcctg gttgcggatc ttcagatcgg tcgcggtgtt gtaaccgcta tagaaaccgt
    12361 ctttcgtgct ctcgaagccg gaaaagtgca gccccagttc gtagcgcgcg atctcgccgg
    12421 tcttggtgtc gccgagcagc cagctgttcg catagccgcc gttattggcg accgcgaaca
    12481 tctcgcacca ttcgccgatg tcgttggcgt actgcgacgc gcgccgcgag cgatagaact
    12541 ccggcgcacc ggccgcgttg tagccgacga agttggagat ggtggtttca gtgactatca
    12601 ggcccgcttc cgtgacccag aaatcggtga ggctggagat gcagccgggc aagccttgca
    12661 tcaggatgcg cttgccgcga tccgggacga tgtcgaacac gacgttgaac gcgtcgcccg
    12721 cggcgtagcg gtcccacgag ttgtgcgcca tcacgatgcg gccgtcgcgg gtggccttgc
    12781 cggtggcgat gaacgcgctg caatgatggc cacggcgccc acgccacggt ttgaggccga
    12841 gctgcggttg ccgcgccgcc gcgtggctcg gccaccagct ttgcagcaga tccatgtagc
    12901 cgttccaggc cagcacttcg gcgaacggca gcttcgcgcc gtcggcgatg ccctggattt
    12961 cgtcggcgaa ttcggtgtcg agcttgttgg cgaactggga tgtggcggca tcgacgaagg
    13021 tgtcgaagtc ttcgccggtg tcccatttcg cgaggtaacg ggcggtgtgg atcgcgttgc
    13081 ggatttcagt ggcgagcaac tgaccgtgct gctcgccgcg atcgtacggc tcgccttcga
    13141 tatggagaaa aatccagccc gcctgatcgc ggcgcacggc atcgagggac ggttcgctca
    13201 tcttcgctcc ggtcgaaggg cgggcgtctc gtgcgcccgt cctggcgtaa cgcgcgctgc
    13261 tcgcaatcgc tgcagcggtt ttttcattgt agagaatctg ccgcgctttg cacgggctgg
    13321 gaccgcaggc ggcgtgggtg agacgccgcg caatgccgcg cgggcttggc caatgcctca
    13381 gcaatcagac gactcagtac acgaatgccc gaaccgcagc ctcgcgatgc ccgaaccgca
    13441 gcctagcgcg tcggattcat gcggaaatac gcatcgagca gcgaggtctt atagaccacc
    13501 cccgcaagct ttggatccgc ggtgctttcg accaccggca gccgctcgcc ctgaaacgcc
    13561 atgaagtgtt gcaaggcaac gccgagcggc atatccggcg tcagcacatc gaaatgcggc
    13621 tgcagataat ccgcggcggt tttcgtcgcg gtctcgcgct tgtcgagcag atcggaggtg
    13681 atgtccttga gcgcgaccac gccgagaaac gcgccggacg cgttggcaac gtacagatac
    13741 ttcaccggat attcgaggaa cacgcgcgtc atgtcctgca cgctcgcatt cggcggcacg
    13801 acggtttcgg cgggccggat cagttcgcgc atttgcgtcg cgcgcagccg ggtgcgcgcc
    13861 tgctcctcct gattgcggcg cagcgtcacc tcgtacatcg aggtcttgcc gatcgcgcgc
    13921 gagacgaaat acgccaccac gcacgacagc atcagcggca gcaccacctg atagctgagc
    13981 gtcatttcga agatcaacag gatcgccatc agcggcgcct gcgtggcgcc ggccagaaac
    14041 gcgcccatgc cgaccatcgc gtaggcgaac ggcgccgagg tgccgtgcgg ccacaacgta
    14101 tgcatgccct ggccgaacag gcaacccacc accgcgccaa cgaacagcgt cggcgtaaac
    14161 accccgccga tcgcgcctga acccacggtc gccgacgtcg cgacgatctt gaacaccagc
    14221 acgaggacga gcgcggtcca tgtccacggc gaatgcagga tcgagttgac gacgctatag
    14281 ccgttgcccc acacctcggg cgtcca
//
LOCUS       DATASH010000746         9068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4276566,
            whole genome shotgun sequence.
ACCESSION   DATASH010000746 DATASH010000000
VERSION     DATASH010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 9068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9068
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4276566"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            410..1651
                     /locus_tag="VHV99_19910"
     CDS             410..1651
                     /locus_tag="VHV99_19910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HEX3637706.1"
                     /translation="MNAVQTPLTAEAGHPPPYQPLGLAALLRAATAGNDLTPLGERLL
                     AYSERYEDPYALLELSLVLQLKYQKASALTVQNLAIQMRKHYRLKSAHSAEAPVKVLV
                     LKSPGDLMANTPFECLVENADLQVDVLYVDGDLPQDASLPDHDVILVAACALDENAKP
                     LARIAALTRRADCRVLNRPERIAHTTRDAAYALLGKVPGVCMAHTVRLSRERILEAAS
                     GSFDLSDVLDGQYPFIVRPVGSHAGQGLVKVSDGLDLARYVDGTDVQEYYVAPFIDYS
                     SADGLFRKYRIVMIEGAPFVCHMGISREWMVHYPYVEMIAHPERREEEARLMASFDSD
                     FAVRHREAFRMIAETTDLDYVGFDCAETSDGRLLIFEIATAMVVHDMDDPDTFPYKAS
                     QMRRVFDAFHQMLRRAATGSC"
     gene            1678..2772
                     /locus_tag="VHV99_19915"
     CDS             1678..2772
                     /locus_tag="VHV99_19915"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017773662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEX3637707.1"
                     /translation="MKSNTLNFSGGPGALPETVLEQTREAVARLPETGISVLGMSHRS
                     DWFQSILNEAEQNLRSLLGISDDYAVTFLQGGSSLQFAMIPMNFATKASAPPEYVTSG
                     YWSGRATSEAAKVTARKVAWDGHATGYRQLPDLQALDVSNSAAYLHYVSNETVEGLQF
                     PNCEDEMPVPLIADMSSDFLSKPFRPDLFSMVYAHAQKNLGPAGVTVAVIKRSLLEQI
                     PDGLPAILDFRTHVSHGSNYNTPPVFAIYVMTLITRWLRFEIGGLHSMQRINERKAKR
                     LYETFDALGDAVTIHADRPWRSQMNVAFTFGDARLDDAFVETALEQGIVGLEGHRSIG
                     GLRASLYNAVTEEAVDILTNALTEFSLQRV"
     gene            3085..4026
                     /locus_tag="VHV99_19920"
     CDS             3085..4026
                     /locus_tag="VHV99_19920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018418802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX3637708.1"
                     /translation="MQKRRIGRSELEVAPLMFGGNVFGWTADEATSFSILDAFVDAGL
                     NFIDTADVYSAWAPGNQGGESETIIGKWFRQSGKRDKIVLATKVSKHPQRKGLSAANI
                     QAAVEDSLRRLQTDYIDVYFSHDDDAETPLAETLGAYQKLIEAGKVRVIGASNYSGAR
                     VEEALAVSRQHGLPEYQLLQPEYNLYDRAEYERDTEPVALANQLGVVVYYSLASGFLS
                     GKYRSKADLANKTRGSRVEKYLNERGLRILDTLDRVAERHGSTPATVALAWLIARPSV
                     TAPIASATSVEQLKSLAASVHLMLTGADIRELDEASA"
     gene            4385..4597
                     /gene="rpsU"
                     /locus_tag="VHV99_19925"
     CDS             4385..4597
                     /gene="rpsU"
                     /locus_tag="VHV99_19925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004198205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEX3637709.1"
                     /translation="MTTILLKENEPFEVAIRRFRRAIEKNGLIAELRERQSYEKPTTA
                     RKRKKAAAVKRLHKRLRSQMLPKKLH"
     gene            complement(4873..5469)
                     /locus_tag="VHV99_19930"
     CDS             complement(4873..5469)
                     /locus_tag="VHV99_19930"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018432488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEX3637710.1"
                     /translation="MTQRCNWVSSEALAHYHDTELGVPSRDDQHLFELLVLEGAQAGL
                     SWSTILNKRTGYRRAFADFDIDKVARLTPKHVDALVLDESIVRHRGKIESAITNARAV
                     QQIQAEHGSLANFVWSFVDQTPIQNDWTSYKQAPASTEVSDALSKGLKRYGCKFVGST
                     ICYAFMQAVGMVNDHEASCMCRARCAALGKKGRKRKAG"
     gene            5610..6491
                     /locus_tag="VHV99_19935"
     CDS             5610..6491
                     /locus_tag="VHV99_19935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEX3637711.1"
                     /translation="MNKDLFLIGIDGGGSGTRVVLGDAQGRELAQAASGPSGLGLGVE
                     RAWLAIEAACADAFARAGVPLDWARCVLGCGLAGVNNRDWLAAFRSQAPAVAGLAVES
                     DVYTSLLGAHGGAPGVVVALGTGSQAAVLDSNGECRVAGGFGYPSGDEASGAWLGLRV
                     IVHAQQSLDGRVPKDDLAQALLAHAGAHDYDSLVVWLCEANQTAYARLAPIVIAHRTH
                     PFAARLLSEAGVEVGKMIAALDPSATLPVALCGGLGAPLREYVPQIYQSRLREPLADS
                     AHGGLQLAQREAVRIGG"
     gene            6579..7400
                     /locus_tag="VHV99_19940"
     CDS             6579..7400
                     /locus_tag="VHV99_19940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEX3637712.1"
                     /translation="MYKVIATDLDGTLLNANHQLDPFTIATVRKLESDGLHFVIATGR
                     HYCDVAGIRDVLGINPYLITSNGARIHAPDNTVIHADDLPPAIVQRLVQPEIVGAHGR
                     VIVNVFTENAWLIDRDAPDLLKFHQDSGFTYAVTDLPKHDGVDIAKVLYIGEPADLAH
                     VAANLAREFGDALYVTYSLPDCLEVMTSNVSKGRALQIVLERLGIGASQCVAFGDNMN
                     DIDLLETAGHPFMMNNANPDLITRLPDVPRIGNNFEAGVAHHLRKLFSLDDELMS"
     gene            complement(7487..7783)
                     /locus_tag="VHV99_19945"
     CDS             complement(7487..7783)
                     /locus_tag="VHV99_19945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PXPV repeat protein"
                     /protein_id="HEX3637713.1"
                     /translation="MKRIVTHLLVVAGLAAGACGVAQAHTDLNFGLSLGAPVYAQPAP
                     VYAAPQPVYYGNGGWDHRYYHGDRGDNRGWDRHDRHDRHWGRDDRGDRRGGWHD"
     gene            complement(8069..8764)
                     /locus_tag="VHV99_19950"
     CDS             complement(8069..8764)
                     /locus_tag="VHV99_19950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX3637714.1"
                     /translation="MTRFHGFLHGVALSIGALCAGVLALPAAHAADGNTETLVFVRHG
                     EKPAKGYGQLDCQGLNRALALPAVIAAKFGKPDLIYAPDPGQQKDDSGHSYYYVRPLA
                     TIEPTAIQFQMPVQTPYGFAQIDQLGTALVNPANRNKLIVVAWEHKLIVKMLRQMMSA
                     HGGNAADVPNWQSDDFDSIYIVRLRWQNGTAHASFKLDHQGLNGRAADCPCAALPGAP
                     SAAATGASAGSAD"
     gene            complement(8910..>9068)
                     /locus_tag="VHV99_19955"
     CDS             complement(8910..>9068)
                     /locus_tag="VHV99_19955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEX3637715.1"
                     /translation="PISGPALFVGGAAMDQLWMFWVAPIIGAVIAGVLYPVIAESRRG
                     NSQELALD"
BASE COUNT         1704 a         2683 c         2806 g         1875 t
ORIGIN      
        1 ggtgtggatt ctgctgccgc actttccgga ttggcgctgg atgcacgatc gggatgacac
       61 gccctggtac ccgagcgcgc ggcttttccg ccagacggta gcgcgtgatt gggacggggt
      121 agtcaagcga gtgcgcgacg agctttcgtt acgagtcgcg cggttcactt gaataggcgg
      181 ctatttcgtc ctctgttcct tctttgtgtt ccggccgatt ccagtagtct atgttggggc
      241 tcgatctgaa atctcagcgc aggcagcgtc gtggctccgc ttgtgccgaa ccggacgctc
      301 gtgctctcac gcgtgcccgt ccatcgcggc ttgactgaac catgcgtaag acaatggacg
      361 tgaaggtaac gcctctcacc ctgtatcact ttatcgaagc acctcatcca tgaatgctgt
      421 tcaaacaccg ctcacggcgg aggcagggca cccaccgcct tatcagccgt tgggtctggc
      481 cgctttgctc agagcagcaa cggcgggaaa tgatctcacg ccgcttgggg aacgcttgct
      541 cgcgtatagc gagcgctatg aggatccata cgccctgctc gagttgtccc tggtgctgca
      601 actcaaatac cagaaagcgt ccgcactcac cgttcaaaac ctggcgattc aaatgcgcaa
      661 gcattaccgg ctaaagagtg cgcactcggc cgaggcgccg gtaaaggtgc tcgtgctcaa
      721 atcccccggt gatctcatgg cgaatacgcc gtttgaatgc ctggttgaga acgccgacct
      781 tcaggtcgac gtgttgtacg ttgatggaga tttgcctcag gacgcgagtt tgccggacca
      841 cgatgtcatc ctcgtcgccg cgtgcgcgtt ggatgaaaac gccaagccgc tcgctcgcat
      901 tgcagcgctg acccgtcgcg ctgattgtcg ggtgctgaac cggcccgaac gcatcgcgca
      961 cacgacgcgc gacgccgcct atgcgctgct tggcaaggtt cccggcgttt gcatggcgca
     1021 caccgtgcgg ctgtcccgcg agcggattct cgaagcggcg tcaggcagct tcgatttgtc
     1081 cgatgtgctg gacggacagt accctttcat tgtgaggccg gtgggctcgc acgccggaca
     1141 agggctggtg aaagtgtctg acgggcttga ccttgcacgt tatgtggacg gcacggacgt
     1201 tcaggaatat tacgtcgcgc cgtttatcga ctacagcagc gcggacggct tgttccgtaa
     1261 gtaccgaatc gtaatgattg agggcgcgcc tttcgtgtgc catatgggga tttcgcgcga
     1321 atggatggtc cactacccgt acgtcgagat gattgcgcac cctgagcgcc gggaagagga
     1381 agcccgcctg atggcaagtt tcgattcgga ctttgcggtg cgtcaccgcg aagcgtttcg
     1441 catgattgct gaaacaaccg atctggacta cgtaggattc gactgcgccg aaacgagcga
     1501 tggccgcctc ctcatcttcg agattgccac cgcgatggtc gtgcatgaca tggacgatcc
     1561 agacacgttt ccttataagg cgtcgcaaat gcgtcgagtc ttcgatgcat ttcaccagat
     1621 gctgcgccgc gcggctacgg gctcatgtta attcaaccga aggcttgttc acgaaccatg
     1681 aaatccaaca cattgaattt ttccggtggt cccggtgcgc ttccggaaac cgtgctggag
     1741 caaacccgtg aggccgttgc gcgtctgccg gagacgggca tatctgtgct cggcatgagc
     1801 catcgatcag actggttcca gtcgatactc aatgaagcag aacagaacct gcgcagcctg
     1861 ctcggcattt ccgatgacta cgcggtcaca tttctgcagg gtggcagcag tctgcagttc
     1921 gcgatgattc caatgaattt cgccacaaag gccagcgcac cgccagaata cgtgacgtcc
     1981 ggctattgga gcggtcgggc gacgtcggaa gcggccaaag tgaccgcgag aaaggtcgcg
     2041 tgggatggtc atgcaacggg ctatcgacaa ctgcctgacc ttcaggcgct ggatgtgtcg
     2101 aactcggccg cttatctgca ctacgtgtcg aacgagaccg tggagggctt gcaatttcct
     2161 aattgtgaag atgagatgcc cgtgccgctt attgcggata tgtcgtctga ttttctgtcg
     2221 aagccgttcc ggccagattt attctcgatg gtgtatgccc atgcacagaa gaacctggga
     2281 cctgcagggg tgacggtggc ggtcatcaaa cggtctcttc tggagcaaat tccggatggc
     2341 ctgccggcca ttctcgactt tcgcacgcat gtgtcacacg gctcgaacta caacacgcca
     2401 ccggtattcg cgatctatgt catgacgttg attacccgtt ggctgcgctt cgagattggc
     2461 ggtttgcatt cgatgcaacg catcaacgaa agaaaggcga agcggctata cgaaacattc
     2521 gacgctttgg gtgatgcggt gacaatccac gcggatcgcc cgtggcgctc gcagatgaac
     2581 gtggcgttca cgtttggcga tgcgcggctc gacgatgcct ttgtcgagac ggcgctggag
     2641 caagggattg tcgggctgga ggggcatcgt tcgattggcg gcttgcgggc ctctctatat
     2701 aacgccgtta cggaggaagc ggtcgatatc ctgacgaatg ccctgacgga atttagtctg
     2761 caacgcgtgt gaggccaacg agcggggctt gcagtcgaat ctcctaaggt tcgccgcacc
     2821 ccgcaattcc ggattgcagt cgcctgcgaa cctgttacgg cgctcgacga gttcgtgcct
     2881 tgacgggaaa tcgtggcagg cgcgccgtcc ggcgtctgcg ggaacagccc ggcgtgggga
     2941 agtcggcgca ccagggcggc tggctgtatc accaagcgcc gccccagata acttaaaagc
     3001 cgtagcgtcg tccaggcgtc gcgctgcaga ggctggcgag acggcgtatt ctcttttcct
     3061 tctctgacaa accctggaaa gatcatgcaa aagcgccgga ttggacgttc ggaattagag
     3121 gtcgcaccgc tcatgttcgg cggcaatgta tttggctgga ccgcggacga agccacctcg
     3181 ttttcgatcc tggatgcgtt cgtcgacgcc gggctcaact ttatcgacac tgccgatgtc
     3241 tattccgcat gggcgccggg caaccagggc ggcgaatcgg aaacgatcat cggcaagtgg
     3301 ttcaggcaga gcggcaaacg cgacaagatc gtgctcgcca ccaaggtctc caagcacccg
     3361 cagcgcaagg gtttgtctgc ggcgaacatt caggcggcgg tagaagactc actgcggcgc
     3421 ttgcagaccg attacatcga cgtctatttt tctcacgacg acgatgctga gacgcctttg
     3481 gccgagacgc tcggcgcgta tcaaaagctg atcgaggcgg gcaaagtgcg agtgatcggt
     3541 gcgtcgaact acagtggcgc gcgcgtcgag gaagccttgg ccgtgtctcg ccagcacggg
     3601 ttgcccgaat atcaattgct gcagccggaa tacaacctgt acgaccgcgc cgagtatgag
     3661 cgtgacaccg aaccggtggc gctcgccaat cagctcggtg tagtggtgta ctacagtctt
     3721 gcaagcggct ttctgtccgg caaataccgc tcgaaggccg atttggccaa caagacgcgc
     3781 ggtagccggg tcgaaaagta tctgaacgag cgcggcttgc gtattctgga caccctcgat
     3841 cgtgtcgccg agcggcacgg cagcacgcca gccaccgtcg cactggcatg gctgatcgca
     3901 cgtccgagcg taacggcccc aattgccagc gcgacctcgg tcgagcagct caagagcctg
     3961 gccgcctccg tccatctgat gctgacgggc gccgatatcc gcgaacttga cgaggcgagc
     4021 gcctgattgt gcgagtggcc tggcgcaacc gctaaacccc tggtaggatg gagagtttcc
     4081 tgatatcatc gggagtgaat tgagatcttt gcctgtttcg tcaatgtttt tcattgatga
     4141 aaggttggcg aaacggcaac agacgtggct ttggtattac gtctgtccgc gttccgcggt
     4201 gaactcccca cctctctttt ccggcctccg tggccgttat gcctctcgtt tcattcgtgg
     4261 tttggccggg tttctacccg cgtttgcctg ttactggcca tgaatcgaaa cgaccggagg
     4321 gccggcgcgt gagcggtatc cgccacgcaa tttaccccta tcaagtgatt gagttaggaa
     4381 ttacatgacg acgattcttc tgaaagaaaa cgagccgttc gaagtggcga ttcgccgctt
     4441 tcgtcgcgca atcgaaaaga atggcctgat cgctgaactg cgtgagcgcc aatcgtacga
     4501 aaagccgacc acggcacgta agcgcaagaa ggctgctgct gtgaagcgcc tgcacaagcg
     4561 cctgcgcagc cagatgctgc cgaaaaagct gcactaagca cgcttttacg cgccgcattc
     4621 cgcacgccga aacggcggtg tgagcttcga aagaagccac atcgccgttt tgcttttggg
     4681 gtgctgaatt gtttttttag gtgctgattt gcgttgggtt tttgcggttg agttatcggc
     4741 ttgtttttgg cgtcaggtag gacgcgccac ggcgggactc gcgcgtggat ggctgtcttt
     4801 cgggctaaag cgggctggtg cggcgcgccg tggcgtgcgc agatcacacg tggtcgaacg
     4861 tgccgctgct tatcagccag ccttgcgttt gcgtcccttc ttgccgagcg ccgcgcaccg
     4921 cgcgcggcac atgcaactcg cctcgtggtc gttcaccatg ccgaccgcct gcatgaacgc
     4981 atagcagatg gtcgagccaa cgaacttgca gccgtagcgc ttcagcccct tgctgagggc
     5041 atccgagact tcggttgatg caggcgcctg tttatacgac gtccaatcgt tctggatcgg
     5101 tgtctgatcg acgaacgacc atacgaagtt ggcgagcgaa ccgtgttcgg cctgaatctg
     5161 ctgcactgcg cgcgcgttgg taatggcgga ttcgatcttg ccgcgatggc gcacgatact
     5221 ttcgtcgagt accagcgcat cgacatgttt aggcgtaagg cgcgctacct tgtcgatgtc
     5281 gaaatcagca aacgcgcggc gatagccggt tcgcttgttg agaatcgtcg accatgacaa
     5341 acccgcctgc gcgccttcca gcacaagcag ttcgaacagg tgctgatcgt cgcgcgaggg
     5401 cacgcccaat tcggtgtcgt ggtagtgtgc gagcgcttcg ctcgataccc agttgcatcg
     5461 ctgtgtcact ttcgcgctcc cgcgttgtca tgtcgcccgt cagcatagcg gaagccctgc
     5521 gcgctgcaag ctggccattc ggccgattgc gggattggaa ctgacctgcc acgctatgcg
     5581 ttattcacca cttgaactca ctcgattcga tgaataaaga cctctttctg atcggcattg
     5641 acggcggcgg cagcggtacg cgggtggtgc tcggcgacgc acagggccgt gaactggcgc
     5701 aggcggcgag cgggccctcg ggtctgggcc tcggcgttga acgcgcgtgg ctggccattg
     5761 aggccgcctg cgccgacgcg tttgcacgtg ccggcgtgcc gctcgactgg gcgcgttgcg
     5821 tactgggttg cggcttggca ggggtcaata atcgcgactg gctggccgcg tttcgctcgc
     5881 aggcgccggc cgtagccggg ctcgcggtgg aaagcgacgt gtataccagc ttgcttggtg
     5941 cgcacggcgg cgcgcccggc gtggtcgttg cgcttggcac cggcagtcag gcggccgtgc
     6001 tcgatagcaa tggcgaatgc cgcgtcgccg gtggcttcgg ttatccgtcg ggcgacgaag
     6061 cgagcggcgc ctggctcggg ctgcgggtca tcgtgcacgc gcagcagtcg ctcgacggac
     6121 gtgtgcccaa agacgatctg gcgcaggcgc tcctcgccca tgccggtgcg catgactacg
     6181 acagcctcgt ggtctggctg tgcgaagcca atcagaccgc ctacgcaagg ctcgcgccga
     6241 tcgtcatcgc gcatcgcacg catccgtttg cggcgcgttt gctcagcgag gccggcgtgg
     6301 aagtgggcaa gatgattgcc gcgctcgacc cttcagcgac cttgccagtc gcgctgtgcg
     6361 gcggcctcgg tgcacccttg cgcgagtatg taccgcagat ctatcagtcc cgtctgcgcg
     6421 agccgttggc ggactccgca cacggcggtt tgcagttggc gcagcgcgaa gcggttcgca
     6481 tcggcggctg agaatcgcct cgcgccccag gtgcgatcgg cccgagacgg cgcgcaacgg
     6541 taaaatgcga tctttgacgc tgcccggctg ctcacactat gtacaaagtt attgccacgg
     6601 atctcgacgg cacgctgctc aacgccaatc accagctgga cccgttcacg atcgctaccg
     6661 tccgtaagct ggaaagcgat gggctgcact tcgtgatcgc gacggggcgc cactactgcg
     6721 acgttgcggg cattcgcgac gtgctgggca tcaacccgta tctgatcacg tcgaacggcg
     6781 cgcgcattca cgcgcccgac aacacggtga tccacgccga cgacctgcct ccggccatcg
     6841 tgcagcggct ggtgcagccc gaaatcgtcg gcgcgcatgg ccgagtgatc gtgaacgtgt
     6901 tcaccgagaa cgcgtggctg atcgacaggg atgcgccgga cctgctcaaa ttccaccagg
     6961 attccggctt cacctatgcg gtcaccgatc tgccgaaaca cgacggcgtc gatatcgcca
     7021 aggtccttta catcggtgag cctgccgatc tcgcgcatgt ggcggccaac ctcgcgcgcg
     7081 aattcggcga cgcgctatac gtcacctatt cgctaccgga ttgtctggag gtgatgacct
     7141 cgaacgtatc gaagggccgt gcgttgcaga tcgtgctcga gcgcctcggt atcggcgcct
     7201 cgcaatgcgt ggccttcggt gacaacatga acgatatcga tctgctggag acagccggcc
     7261 atccgttcat gatgaacaat gccaatcccg atctcatcac acgtctgccg gacgtgccgc
     7321 gcatcggcaa caacttcgaa gcgggcgtcg cgcaccatct gcgcaaactc ttctcgctcg
     7381 acgacgaact gatgtcctga aagcagaccg gcccgcatcc acttgcgtgg ttgcgggccg
     7441 gttgttgcgc cgattctggt tcgcggcctg gcgccgcggg ctacgatcag tcgtgccacc
     7501 cgccacggcg atcgccgcgg tcatcgcggc cccagtggcg gtcgtggcgg tcgtggcgat
     7561 cccagccgcg attatctccg cggtcgccat gatagtaacg atggtcccag ccgccattgc
     7621 cgtaatacac cggctgcggc gcggcgtaga caggggcagg ctgggcataa accggtgcgc
     7681 cgagcgacag gccaaagttc agatccgtat gcgcttgcgc gacaccgcat gcgcccgccg
     7741 cgagtccggc aacaaccagc aagtgagtga cgatgcgttt catattgttc tcctgtgagg
     7801 cgacgtatgt ttgtcgcaca ggaccaatct acgcattccg atcgccgctt actgcgacga
     7861 gatgttacgg actgcaagca tcgtcgcagg gtttgaaagg tggtagaggg aacgttcgag
     7921 cgcatcactc ccgcttcgtt ttccgctcgg agccctgtct tgtaaggctt tgcggcacat
     7981 caaaacggcc cggcgcctgc ttgccaccgc gcgctgcgaa aaacagacag ccgcgcccgt
     8041 aatcgcggct tacatctgta ttacccgact aatcagctga gccagcggaa gcgcccgtcg
     8101 cggccgcgga cggtgcgccc ggcaatgccg cgcatgggca gtccgcggcg cggccgttca
     8161 gtccttgatg gtcgagcttg aaactggcgt gggcggtccc gttttgccac cggagacgaa
     8221 caatataaat actgtcgaag tcgtcgcttt gccaattggg gacgtcagcg gcgttgccac
     8281 catgtgcgct catcatttgg cgcaacattt tcacgatcag cttgtgttcc catgcaacca
     8341 caatcaattt gtttcgattg gcaggattga cgagcgccgt gcccagttgg tcgatctggg
     8401 cgaaaccgta cggcgtttgc accggcatct ggaactggat ggccgtcggc tcgatggtcg
     8461 caagcggccg gacatagtaa taggagtgac cgctgtcgtc cttttgctga ccggggtcgg
     8521 gcgcgtagat cagatccggt ttgccgaact tggcggcgat gacggctggc aacgccagag
     8581 cacggttgag cccttggcaa tcgagctgac cgtaaccctt ggcgggtttc tcaccgtggc
     8641 gcacgaacac gagcgtttca gtattgccgt ccgcggcatg ggctgcgggc aatgccaata
     8701 cgccggcaca caaagcgccg atggagagtg cgacgccatg caggaatccg tggaaacggg
     8761 tcattggtag cacctgtgcg agcttgggaa gtcgcacgat tctagcagcg cgccattggc
     8821 ggattttcaa gccgctcaca ccatgcgcac aaaaacacga cgggcgccgc agcgcccgct
     8881 ttgactggct gaatacgcgc actcgctgat cagtcgagcg ccagttcctg cgagttaccg
     8941 cggcggcttt ctgcgatgac cgggtacagc acgcctgcga tcaccgcgcc gatgatcggc
     9001 gcgacccaga acatccacag ctggtccatc gctgcgccgc cgacgaacaa cgccgggccg
     9061 gagatcgg
//
LOCUS       DATASH010000747         4385 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5663107,
            whole genome shotgun sequence.
ACCESSION   DATASH010000747 DATASH010000000
VERSION     DATASH010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5663107"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="VHV99_19960"
     CDS             complement(<1..772)
                     /locus_tag="VHV99_19960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006406249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX3637716.1"
                     /translation="MLAQTPNRHKTLDVPPVLIAGAGPTGLAAAMSLSRAGIPVRLID
                     KAPQPSPWSRAIGIQARTLELFEQHRLVEPFLELGHRARVANLYSNGQRLARLDFDPL
                     QTRYPYLLFLDQSVTERLMTEHLASFGVRIERGVELMRFRQGSAGLEAAVRHANGAEE
                     SIRPSYLIGADGAHSTVRHGLDIEFTGKTFEQTFLLADLQADTEWPDDEFHIFASGEG
                     IATLFPMGGDRTRLIADKRATSGLAHGLAPALAPDNAAD"
     gene            complement(839..1864)
                     /locus_tag="VHV99_19965"
     CDS             complement(839..1864)
                     /locus_tag="VHV99_19965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX3637717.1"
                     /translation="MEPQEKHQHGHDHEHDHAHGHEHEPGHAHSHGAGGHHHHHAPQA
                     GQGRTFAIAVGLNMLIVVVQAVYGFIAHSTALLADAGHNLSDVLGLLLAWGAVWLGTR
                     KPSERYTFGLGSSSILASLANAALLLFACGAIVLEAVQRLLNPAPVAGLDVFIVATLG
                     MVVNGFSAWLFMRGSKEDLNVRGAFLHMAADAGISAAVAISGLVILFTGASWIDPAMS
                     IIVVGVIVVGTWGLGRDAMRLAMGAVPPGVDKPGIERYLGGVPGVTDVHDLHVWALST
                     TENAMTAHLVMPEGHPGDLFVDGIVATLRRDHAMNHATLQVERGTTEHRCALHERSPA
                     QSQAHSH"
     gene            complement(1865..2197)
                     /locus_tag="VHV99_19970"
     CDS             complement(1865..2197)
                     /locus_tag="VHV99_19970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX3637718.1"
                     /translation="MLSSPFVATDERVVPLADLFRLLGDPTRLRIVLTCVDRRCAVGA
                     IAESLGLSASLVSHHLRLLRAARIVRAEREGKQVFYLAADNHISAMLAELLEHVAEPQ
                     ADFRPDNE"
     gene            2332..4212
                     /locus_tag="VHV99_19975"
     CDS             2332..4212
                     /locus_tag="VHV99_19975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012400131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3637719.1"
                     /translation="MDTVFARGFAAHRDGRLTEAERDYQAALAAEPSHVDALHLLGVL
                     RHQQGQHAEAAELVRRAVDLRPADAGLQLNLGNALKALGRIDDAIERFRNALHLEPGF
                     SLAQYNLGNAYAAAGRHEDAVDAFEKALRLQPLDAATWNNFGNALLGMRRFEEGAKAF
                     RRALQIRPGHAGAHNNLGMALNSLGDTHGAIEQFRAAIAAEPNYVAAHFNLGNLLDAS
                     GHPQDAVPVLQKVLRMQPQFAPAHFGLGHALAATGSHEAALPQFERAVGLDPKYGVAW
                     LGLGNAHLALGAHKPALRAFDQALRLDPSLVAANLNRALTLLALGDYTRGLPAYEWRL
                     RTPGSIALPSLPKWNGEPLPRQTLRILAEQGFGDTLQFVRFVPFAAKLAGQIVLEVQP
                     ELVALLEPAAHAAGVLLKSTAHGLSSGSANATPLADADAFSPLLSLPLALGLSNVDKV
                     PARTPYLVVPAAYRKKWRGSLGGQSKRKLGIAWSGRIQPGETRSAQFDMLEPLFRLAG
                     IDWIVLQPTLTDEERSLLDAHPRARFIQRLEGKLEDFADTAAIVDRLDGVVSVDTAVA
                     HLTGALGKPLWLMLPFAADWRWGIDTASTPWYPKARLFRQPKPGAWDDVIGRVAREIS
                     AS"
BASE COUNT          830 a         1401 c         1374 g          780 t
ORIGIN      
        1 tgtccgccgc gttatcgggt gccaaggcgg gtgccaagcc gtgtgccaag ccggacgtcg
       61 cgcgtttgtc cgcgatcagg cgcgtgcggt caccgcccat cggaaacagg gtcgcgatgc
      121 cctcgccgga cgcgaaaata tggaattcgt cgtcgggcca ttcggtatcg gcttgcagat
      181 cggcgagcag gaacgtttgc tcgaaagtct tgcccgtgaa ctcgatgtcc agaccgtggc
      241 gcaccgtgct atgcgcgccg tcggctccga tcaggtacga cgggcgaatc gattcttcag
      301 cgccgttcgc atgccgcacg gccgcctcca gccccgctga tccttgccgg aaacgcatca
      361 attccacgcc gcgttcaatc cgcacgccga acgaggcaag gtgctccgtc atcaagcgtt
      421 cggtgacgga ttgatcgagg aacagcaggt acggataccg cgtttgcaag ggatcgaagt
      481 cgagccgcgc aaggcgctgg ccattcgaat agaggttcgc gacacgcgcc cgatgcccga
      541 gttcgagaaa cggctcaacc agccgatgct gctcgaacag ttcgagcgtg cgtgcctgga
      601 tgccaatggc gcgtgaccag ggcgagggtt gaggcgcttt gtcgatgagg cgcaccggaa
      661 taccggcgcg ggacaaactc atcgcggcgg cgaggcccgt tggaccggcg ccggcgatca
      721 gaaccggcgg tacgtcgagg gttttgtgtc gattcggcgt ctgcgcgagc atgcacttct
      781 ccctgaaact tcacagtacc cagtgtacgc cggtcgcctc gcgcgaacga ccgctcgctc
      841 aatgcgagtg cgcctgggat tgcgccggcg aacgttcgtg cagcgcgcag cgatgctcgg
      901 tcgtgcctct ttccacctgc agcgtggcat ggttcatcgc gtgatcgcgg cgcaaagtcg
      961 ccacgatgcc gtccacgaac agatcgccgg ggtggccttc cggcatcacc agatgcgcgg
     1021 tcatggcgtt ctccgtggtg gacagcgccc agacatggag atcatggaca tcggtgacac
     1081 ccggcacgcc ccccagatac cgttcgatac ccggcttgtc cacgcccggc gggaccgcgc
     1141 ccattgcaag gcgcatggca tcgcggccaa gtccccacgt gcccaccacg atcacgccga
     1201 ccacaataat gctcatcgcc ggatcgatcc agctcgcgcc ggtgaacagg atcaccaggc
     1261 cgctaatggc aaccgcggcg gagatgccgg catcggcggc catgtgcagg aaagcacccc
     1321 gcacgttcag gtcctccttg ctgccgcgca tgaagagcca tgccgagaaa ccgttcacga
     1381 ccatgccgag cgttgcaacg ataaacacat cgaggcccgc gacgggcgcc ggattgagca
     1441 ggcgctgcac ggcttcgagc acgatcgcac cgcacgcgaa gagcagcagc gcggcgttcg
     1501 cgagcgaggc gagtatcgac gaactgccca gcccgaacgt gtagcgctcc gacggtttgc
     1561 gcgtgccgag ccagacagcg ccccaggcga gcagcaggcc gaggacatcg gagagattgt
     1621 ggccggcgtc ggcaagcagt gcggtggaat gagcgataaa accgtacacg gcctgcacca
     1681 ccacgatcag catgttcagc ccgactgcta tcgcaaacgt gcggccctgc cctgcttgcg
     1741 gcgcgtgatg gtgatggtgg ccaccggcgc cgtggctgtg cgcgtgacct ggctcatgct
     1801 cgtgaccatg agcatggtcg tgctcatggt catgcccgtg ctggtgtttc tcttgtggtt
     1861 ccatttattc gttatccggt cgaaagtcgg cctgcggctc ggcaacatgc tcgagcaatt
     1921 cggccagcat ggcgctgatg tggttatcgg cggcgagata gaaaacctgc ttgccttcac
     1981 gctcggcgcg tacaatccgc gccgcccgca gcaaacgcag gtgatgactg acgagcgagg
     2041 ccgacaaccc cagcgattcc gctattgcgc ccaccgcgca gcgccggtca acgcacgtca
     2101 gcacaatgcg caagcgcgtg ggatcgccca agaggcggaa caggtcggca agcggaacaa
     2161 cgcgttcatc agttgctaca aagggagaag acaaaataga tcgaccaaca tttgaacaag
     2221 tgttcaaatg ttacagagat gaaccccggt gcgtcaacgc aaaattccgc aaaactcggg
     2281 atcagtgtcg aacgtgtgta aaaatgaggc gtttgcattc gattttcctc aatggataca
     2341 gtttttgcgc gtggcttcgc ggcgcatcgc gatggccgcc tcaccgaagc ggaacgcgac
     2401 tatcaagccg cgcttgccgc agagccctcc cacgtcgacg cacttcatct gctcggcgtg
     2461 ctgcgccatc aacaaggcca gcacgcggaa gcggccgaac tggtgcgtcg cgcggtggac
     2521 ctgcgccccg ccgacgccgg tctgcaactc aatcttggca acgcgctgaa ggcgctgggc
     2581 cggattgacg acgccatcga gcgattccgc aacgcgctgc atctggaacc cggtttttcg
     2641 cttgcgcagt acaacctcgg caatgcatac gcggcggcag gccgtcacga agacgccgtc
     2701 gatgccttcg aaaaagcgct gcgtctgcaa ccgctggatg ccgcgacctg gaacaacttc
     2761 ggcaatgcgc tgctcggcat gcgccgtttc gaagaaggcg cgaaggcatt tcgccgtgcg
     2821 ctgcagattc gtcccggcca cgcgggcgcg cacaacaatc tcggcatggc gctcaattcg
     2881 ctgggagaca cccacggcgc gatcgaacaa tttcgcgccg ccattgcggc cgagccgaac
     2941 tatgtcgcgg cgcatttcaa cctgggcaat ctcctcgatg cctccggcca cccgcaagac
     3001 gccgtgccgg tgctccagaa agtactgcgt atgcagccgc aattcgcacc ggcgcacttc
     3061 ggcctgggtc acgcgcttgc ggctaccggc tcgcacgaag cggcgttgcc gcaattcgaa
     3121 cgcgcggtcg ggctcgatcc gaagtacgga gtagcgtggc ttggccttgg caacgcgcat
     3181 ctggcgctcg gcgcgcacaa acccgcgttg cgtgcgttcg atcaggcctt gcggctcgat
     3241 cccagccttg tcgcggcgaa cctgaaccgg gcgctgactc tgcttgcatt aggcgattac
     3301 acgcgcggcc tgcccgccta cgaatggcgc ctgcgcacgc cgggcagcat tgcgctgccg
     3361 tcgctgccga agtggaacgg tgagccgctg ccgcgccaga cgttgcggat tctcgcggaa
     3421 caaggtttcg gcgataccct gcagttcgtc cgtttcgtcc ccttcgcggc gaagctcgcc
     3481 ggacagatcg tgcttgaagt tcaaccggaa ctcgtcgcgt tgctggagcc ggcggcgcac
     3541 gctgcgggcg ttttgctgaa gagcaccgcc catggtttgt ccagcggctc agccaacgcc
     3601 accccgctcg ccgatgcaga cgctttctcg ccgttgttga gcttgcccct ggcgctgggt
     3661 ctgtcgaacg tcgacaaggt gcccgcccgc acgccgtatc tcgtggtgcc ggcggcttat
     3721 cggaagaagt ggcgcggttc gctcgggggt caatcgaaac gcaagctggg cattgcatgg
     3781 tccgggcgca ttcagccggg agaaacccgc tcggcgcagt ttgacatgct cgaaccgttg
     3841 ttcaggctgg cgggcatcga ctggatcgtc ttgcagccca cgttgacgga cgaagagcgc
     3901 tcgttgctgg acgctcatcc gcgcgcccgc ttcatccagc gactggaagg caagcttgaa
     3961 gatttcgcgg atacggccgc gatcgtggat cggctggacg gtgtcgtatc cgtcgatacc
     4021 gccgtcgctc atctgaccgg cgcgttagga aaaccgttat ggctgatgct gccgttcgcg
     4081 gctgactggc gctggggtat cgataccgcg tcgactccgt ggtacccgaa agcacgcctg
     4141 ttccggcaac caaaacccgg cgcatgggac gatgtgatcg gacgggtggc gcgcgaaata
     4201 agcgcttcgt agaacgggtt cagaagcctt gaacgcacaa acaaaaatcc ctcggcattg
     4261 tcgagggatt ttttgctttc aaagccgcac tatcacgccg tcttgtactg actgcgcgct
     4321 tccgtggatc gatacaaaac cagcgtcgcg atcagcccgc agaccgcggc aacggccatc
     4381 catgc
//
LOCUS       DATASH010000748         5546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_518193,
            whole genome shotgun sequence.
ACCESSION   DATASH010000748 DATASH010000000
VERSION     DATASH010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5546
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518193"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..1427)
                     /locus_tag="VHV99_19980"
     CDS             complement(180..1427)
                     /locus_tag="VHV99_19980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX3637720.1"
                     /translation="MATLITDVKVILTAPEGINLIVVKVETNQPGLVGLGCATFAYRH
                     VAVKCLIEAYLRPLLIGRDAEAIEELWQLMHQNAYWRNGPIENNAISGVDMALWDIKG
                     KQANMPLYQLFGGKCREGVPIYRHADGRDLNELCENIQKYREQGITHIRCQSGGYGGG
                     GYGQAPSGAPSGSAHGVYLDSRKYMRETLKLFDGIRSKLGFDVALCHDVHERLKPVEA
                     IRFACELEQYDLFFLEDAIPLEEGEWMRQLRAKTTTPLAQGELFNNPYEWRFLITERL
                     VDYIRVHLSQIGGITAARKLQIFAEQFGVRTAWHGPGDMSPMAHAANIHIDLASRNFG
                     VQEWSGTEPPNFVIQELKGPGAALLDVFPGLPEFRDGYVYANDKPGLGVDIDEAEAAK
                     YPCENTVTTWTQTRLKDGSLQTP"
     gene            complement(1480..2772)
                     /locus_tag="VHV99_19985"
     CDS             complement(1480..2772)
                     /locus_tag="VHV99_19985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3637721.1"
                     /translation="MESVKPVAPQRWWYLMPIIFITYSLAYLDRANYGFAAAAGIDKD
                     LGITHGTSSLIGSLFFLGYCLFQVPGAIYAQRNSVKKLIFFSLILWGLCAAATGMVSN
                     IPMLMVLRFTLGVVEAAVMPSMLMYISRWFTRTERSRANTFLILGNPVTVLWMSVVSG
                     YLVRSFGWREMFVFEGAPALIWAVVWWFTVKDRPADAPWMSEAEKSELIARLKAEQAD
                     IAPVRDYKSAFRSSIVMKFCAIHALWSIGVYGFIMWLPSILKAASTIDIVSVGWLAAV
                     PYLAAIILMLLASWLSDKTRNRKLFVWPLLLVGTVAFVASYLIGGSHFWISFALLVVA
                     GATMYAPYGPFFALVPELIPSNVLGGAIGLINACGALGAFAGSWVVGYLNGATGSPAA
                     SYIFMAGGLLLSVILMMTVPAHSGQRAKREAPLSLNRT"
     gene            complement(2869..3636)
                     /locus_tag="VHV99_19990"
     CDS             complement(2869..3636)
                     /locus_tag="VHV99_19990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007581097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3637722.1"
                     /translation="MTHLFDLNGRTALVTGSARGIGYALAEGLAAAGASVVLNGTRAD
                     TVAESVSKLVGNGYQAQGRAFDVTDEVAVAEAFAQWDKDGVQIDIVVNNAGIQFRKPL
                     VELELSDWQRVIDTNLTSAFIVAKQAARRMIARGTGGKIINIGSLTSEAGRATVGAYT
                     AAKGGIKMLTRAMSAEWAASDIQANAIGPGYILTDMNKPLIENATFDAWVRSSNPSQR
                     WGRPEELVGTAVYLASSASSYVNGQIIYVDGGWLAVL"
     gene            complement(3746..4465)
                     /locus_tag="VHV99_19995"
     CDS             complement(3746..4465)
                     /locus_tag="VHV99_19995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016347174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEX3637723.1"
                     /translation="MATLHRQVLNQVGEQICSGKFLPGDILPSEDVLAEQMQVSRITI
                     RETMKSLSAKGMLQVRRRYGTIVLPRAQWQLFDPDVITWRARAGAIEPGLIEDLMELR
                     LIIEPNAARLAAKRATPEDRIAVRRAFKAMERAVAGHGEYVPADLAFHGAILAACHNQ
                     FVQQMQNALSAILRTSFELSSEIAGGPARSLPMHEALCVAIEQGDEVAAEQAVLTLIK
                     RAEQDFEDRAALNNTASDQPA"
     gene            4745..5014
                     /locus_tag="VHV99_20000"
     CDS             4745..5014
                     /locus_tag="VHV99_20000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010098346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEX3637724.1"
                     /translation="MKFSSQIKPISYLKSHAAQIVSEIADSRQPLLITQNGEAKLVVM
                     DVHSYEEQEQTMALLKILAMGSKEIQQGKFRDAEDVIADLDRSEE"
BASE COUNT         1119 a         1733 c         1603 g         1091 t
ORIGIN      
        1 atccggttta cggggatcca tccgacgcga tccaggagaa tccgcctgct gccgacagtt
       61 ttgcgggtaa attgccggca aattcacgat agctatccag tcccgattcc acgtccacgc
      121 cgatgggcgc tttcatcccg aactacccgc cagacctttc tgtatctcat gggagcacat
      181 cacggtgttt gcaacgagcc gtctttcaat ctcgtctgtg tccacgtggt gacagtgttc
      241 tcgcacggat acttcgccgc ctcggcttcg tcgatgtcca cgccgagccc gggcttgtca
      301 ttggcgtaga cgtagccgtc ccggaactcc ggaagccccg ggaatacgtc gagcagtgcg
      361 gctcccggac ctttgagttc ctggatcacg aagtttggcg gctcggtgcc ggaccattcc
      421 tgcacaccga aattgcgcga agccagatcg atgtggatgt tggccgcatg cgccatcggc
      481 gacatgtcgc ccggtccatg ccacgccgtc ctcacaccga actgctcggc aaatatctgc
      541 agcttgcgag ctgcggtgat gccgccgatc tgactcagat gcacgcggat atagtcgacg
      601 agccgttcgg taatcaggaa gcgccattca tacggattgt tgaagagttc gccctgagcc
      661 aacggtgtgg tcgtcttcgc gcgcagttgc cgcatccatt cgccctcctc cagcgggatt
      721 gcgtcttcga gaaagaacag gtcgtactgt tccagctcgc acgcaaagcg gatcgcttcg
      781 accggtttca gacgttcgtg tacgtcgtga cacaacgcga cgtcaaagcc gagcttgctg
      841 cgaatgccgt cgaacagctt gagcgtttcg cgcatgtact tcctgctgtc gagatacacc
      901 ccgtgcgccg aacctgacgg cgcgcccgac ggcgcctggc catagccccc cccgccataa
      961 ccgccgctct gacaacgaat atgcgtgatc ccctgctcgc gatacttctg gatgttctcg
     1021 cagagttcat tgagatcgcg gccatcggca tgccggtaaa tcggcacgcc ttcacggcat
     1081 ttgccgccga acaactgata caacggcatg ttcgcctgct tgcccttgat atcccacagc
     1141 gccatgtcga cgccggaaat tgcattgttc tcgatcggcc cgttacgcca gtacgcgttc
     1201 tggtgcatca gttgccatag ctcctcgatc gcctcagcat cgcggccgat cagcagcggc
     1261 cgcagatacg cctcgatgag gcacttcacg gcgacgtgcc ggtaagcgaa cgtcgcgcag
     1321 ccgaggccga ccagacccgg ctgatttgtc tccaccttca cgacgatcag gttgatgcct
     1381 tccggcgcgg tcaggatgac ctttacatcg gtaatcaggg ttgccatttt gagcctgcca
     1441 gaaacgaatc aaagagaacc acgcggcccg atcagcgctt tacgtgcgat tcagcgacag
     1501 cggcgcttca cgcttcgcac gctgaccgga atgagcgggc acggtcatca tgagaatcac
     1561 ggagagcagc aagccgcccg ccatgaaaat gtatgaagcc gccgggctgc cggtcgcgcc
     1621 gttcagataa cccaccaccc atgaacccgc aaaagcaccg agggcgccgc atgcgttgat
     1681 gagaccgatc gcgccgccca gcacgttgct cggaatcaac tccggcacca gcgcgaagaa
     1741 cgggccgtac ggcgcataca tcgttgcgcc cgccacaacc agcagtgcga acgagatcca
     1801 gaaatgcgag ccgccgatca gatacgaggc gacgaacgca accgtgccca ccagcagcaa
     1861 cggccacacg aacagcttgc gattgcgtgt cttgtccgaa agccacgagg cgagcagcat
     1921 gaggatgatc gcggcgagat acggcacggc ggcgagccag ccgaccgaga cgatatcgat
     1981 cgtggacgcg gccttgagga tcgacggcaa ccacatgatg aagccgtaca cgccgatgct
     2041 ccagagcgca tgaatcgcgc agaacttcat cacgatcgac gaacggaacg ccgacttgta
     2101 gtcgcgcacc ggcgcgatgt cggcctgctc ggctttcaga cgtgcgatga gttcggactt
     2161 ttcggcttcg ctcatccacg gcgcgtcggc cggacgatcc ttgacggtga accaccacac
     2221 tacggcccag atgagcgccg gcgcaccttc gaaaacaaac atttcgcgcc agccgaagct
     2281 gcgcaccaga tagcccgaca ccaccgacat ccacaacacg gtcaccggat tgccgagaat
     2341 caggaaggtg ttggctcgcg agcgctcggt cctggtgaac cagcggctga tgtacatcag
     2401 catcgacggc atcaccgccg cttctaccac accgagcgtg aaacgcaaca ccatcagcat
     2461 cggaatattg ctgaccatgc cggttgcggc tgcgcacagt ccccacagaa tcaggctgaa
     2521 gaagatcagc ttcttcacgc tgttgcgctg cgcgtagatt gcgccgggca cctgaaacag
     2581 gcagtagccg agaaagaaca gggagccgat cagcgacgac gtgccgtgcg tgatgccgag
     2641 atccttgtcg atccctgccg ccgcggcaaa gccgtagttc gcgcggtcga gataggcgag
     2701 gctgtatgtg atgaagatga tgggcatcag ataccaccat ctttggggtg ctacaggctt
     2761 gacgctttcc atgatgtctc cggatcggta cggcgtatac aacgattgac tggcgcaagt
     2821 cgttttattg ttctgctgta ctcgcgtggg cctgctttta aagcgacttc aaagtacggc
     2881 gagccagccg ccgtccacgt aaatgatctg accgttcaca tagctggacg cggacgacgc
     2941 gagatacacc gccgtgccga cgagttcctc cggtctgccc cagcgttgtg acggattgct
     3001 gctcctgacc catgcgtcga acgtcgcgtt ctcgatcagc ggcttgttca tgtcggtcag
     3061 gatgtagccc gggccgatcg cgttcgcctg gatgtccgag gccgcccact cggcgctcat
     3121 tgcgcgggtc agcatcttga tgccgccctt cgccgccgtg tacgcgccga ccgtcgcgcg
     3181 gcctgcttcg ctggtgagcg agccgatgtt gatgatcttg ccacccgtgc cccgcgcgat
     3241 catgcgtcgc gccgcctgct tcgcgacgat gaacgcgctc gtcagattgg tgtcgatcac
     3301 gcgctgccag tcgctcagtt caagctcgac gagcggcttg cggaactgga tgccagcgtt
     3361 gttcacgacg atgtcgatct gcacgccgtc cttgtcccac tgcgcgaaag cctcggcgac
     3421 agcgacttcg tcggtgacgt cgaaagcacg gccctgcgcc tggtatccgt tgccgacgag
     3481 tttgctgacc gattcagcaa ccgtatccgc acgcgtgccg ttgaggacga cgctggctcc
     3541 cgccgccgcc agtccctcgg ccagcgcgta gccaataccg cgcgccgagc cggtgacaag
     3601 cgccgtacgg ccgttcagat cgaatagatg agtcatgctg gatcctctat gtgttcagga
     3661 aaaaggcgcg agtgcgccca tcgcgatggt tttccgcatc gcgttacaag tcggtcaggt
     3721 ttgctattgg cctggcaggc ctccgtcagg ccggttgatc gctggcggtg ttgttcaatg
     3781 cagcccggtc ctcgaaatcc tgctcggcgc gcttgatcag cgtcagtact gcctgttcgg
     3841 ccgccacctc gtcgccctgc tcgatcgcca cgcacagtgc ctcatgcatc ggcagcgaac
     3901 gagctgggcc gccggcgatt tctgaactga gttcgaagct ggtacgcagg atcgccgaca
     3961 gcgcgttttg catctgctgg acgaactggt tgtgacaggc tgcaaggatg gcgccgtgaa
     4021 acgcgaggtc ggcgggaaca tattcgccgt ggccggccac cgcgcgctcc attgctttga
     4081 aggctcgccg cacggcgatg cgatcttccg gcgtcgcgcg tttcgcggcg agtctggccg
     4141 cgttcggctc gatgatcagg cgcaactcca tcagatcttc gataagaccc ggctcgatcg
     4201 cgccggcgcg cgcacgccag gtgatcacgt cgggatcgaa gagttgccat tgcgcgcgcg
     4261 gcagcacgat cgtgccatag cggcggcgca cctgcagcat gcccttggcc gagagcgact
     4321 tcatcgtttc gcggatggtg atgcggctca cctgcatctg ttcggcgagc acgtcctcag
     4381 acgggagaat gtcgccgggc aggaatttgc ctgaacagat ctgctcgccc acctgattca
     4441 ggacctgtct atgtagtgtc gccacaattc accctctagg tcatacgtat gatctagaag
     4501 ataggcgtga aggcacactt gcgcaaccag ggttccccct gcgggtgcat gagcgccatg
     4561 aagctccccg cccgctacta ttgaacgcca gggccgcaag acgatctcta taagctccgt
     4621 gactgcggct ccctgtatct gctggtctcg gcagcgggcg cgaagacatg aatggtttgg
     4681 ctataaactc aagacatatt cggactaaaa ccagtcttac acgcctacgc ccttagtgcc
     4741 gcccatgaaa ttctcgtcgc agatcaagcc aatcagctac ctcaaaagcc atgctgcgca
     4801 gattgtcagc gagattgctg acagccgcca accgctgctg atcacacaga atggcgaagc
     4861 aaaactcgtc gtgatggatg tgcacagcta tgaggagcag gaacagacta tggcgctcct
     4921 gaagatcctt gcgatgggtt cgaaagagat ccagcaaggg aaattccggg acgctgaaga
     4981 cgtcatcgct gatctggacc gatccgaaga atgacgcatc gcgttgttac tctcgggtcc
     5041 gcggaagacg atatacgtga aatccgacac tacgtcgtca gcccgttcgg tcaaagcaca
     5101 tgggggcgga cctggtcggc gctgaaacag acaattcgtc ggctagctga tttccctgat
     5161 tctggtggac gggtcgacga gttgcgtgag ctgaacatga ccgagtttcg acagacaatc
     5221 tccggcgcgc acagggtcat ctaccaggtt gcagacaaca tggtctacgt ccacattgtt
     5281 tgcgatctcc gtatggatct cacagcccta ctccatcggc gcttgctccg accgatttag
     5341 cctgcttcat gcggagttgc gtgctctccc atctgggtca aacgcccgat catcggcagc
     5401 gcaaccgtca aacgtcgcgc agcgccagtt cgacatgttt gaggcgtcgc acaaccactc
     5461 gcaggacagc agtccgaaat ggcatcaggt gaatgccggc gcggactact tcctgagcaa
     5521 gagcaccgat ctcttcctcg tgggga
//
LOCUS       DATASH010000749         2735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2890629,
            whole genome shotgun sequence.
ACCESSION   DATASH010000749 DATASH010000000
VERSION     DATASH010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2890629"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2023
                     /gene="xdhB"
                     /locus_tag="VHV99_20005"
     CDS             <1..2023
                     /gene="xdhB"
                     /locus_tag="VHV99_20005"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdopterin binding
                     subunit"
                     /protein_id="HEX3637725.1"
                     /translation="VRYEPLEVILTPAEAKARKQFVLPPLHLKRGEPDAKIAAAPHKI
                     RGTFEVGGQEQFYLEGQIAYAVPKEMDSMLVYSSTQHPSEMQQVVAHMLGWPAHSVVC
                     ECRRMGGGFGGKESQSALFACVASLAAKLLHRPVKLRADRDDDFMITGKRHDAVYEYE
                     AGFDDSGRILGARVEIALRAGYSADLSGAVATRAVCHFDNAYYLSDVDIVALCCKTNT
                     QSNTAFRGFGGPQGALVMEVMLDSIARQLKCDPLDVRLANYYGIGERDTTPYGQRVED
                     NIIAPLTDALLKTSDYRARRAAIAAFNGKSPVLKRGLAFSPVKFGISFNVPFLNQAGA
                     LVHVYKDGSVLVNHGGTEMGQGLNTKVAQVVANQFGLPLSRVRVTATDTSKIANTSAT
                     AASTGSDLNGKAAEAAARTIRNRLAELAAKQLGGTADAVEFTHGEVSVNGGAMPFEQL
                     VGAAYLARVQLWSDGFYTTPKVHWDAKTLTGHPFYYFAYGAAVSEVVIDTLTGEWKLV
                     RADVLHDAGQSINPAIDIGQVEGGFIQGMGWLTTEELWWNRDGRLMTHAPSTYKIPAV
                     SDTPAAFHVQLYQNQNAEPTVFHSKAVGEPPLLLPFSVFLAIRDAVAAAVPGAEHAPA
                     LRAPATPEAILDALDALQPPGTTMQPATEQPAKQPTEAAVTATTVTTPV"
     gene            2151..>2735
                     /locus_tag="VHV99_20010"
     CDS             2151..>2735
                     /locus_tag="VHV99_20010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018434439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEX3637726.1"
                     /translation="MQAWLPDLQQLLAHGDAAVLVTVARVEGSAPREAGTKMIVTRDS
                     AKHTIGGGHLEWKAIETARQVLRDGMRSPHMRRLERFALGPSLGQCCGGAVILAFERL
                     DIGDLGWITSLAKRVAAGQSTVRSVSFGPAPDAVMLSDPEPGVDASDCLLWDGAGFDD
                     SSALLTETIAPGDFAVVLFGAGHVGAALVRVLATL"
BASE COUNT          523 a          892 c          869 g          451 t
ORIGIN      
        1 cgtgcgatac gagccgctcg aagtcatcct cacgccggcc gaagcgaagg cccgcaagca
       61 gttcgtcctg ccgccgctgc atctgaaacg cggcgagccc gacgccaaga tcgccgccgc
      121 accgcataaa atccgcggca cattcgaagt cggcggccag gagcagtttt atctcgaagg
      181 tcagatcgcg tatgccgtgc cgaaggaaat ggacagcatg ctggtctaca gttcgacaca
      241 gcatccgagc gaaatgcagc aggtggtcgc gcatatgctc ggctggccgg cgcatagcgt
      301 cgtgtgcgaa tgccggcgca tgggtggcgg cttcggcggc aaggaatcgc aatcggcgtt
      361 gtttgcatgc gtggcgtcgc tggcggcgaa actcctgcac cgcccggtca agctacgtgc
      421 cgatcgcgac gacgatttca tgatcaccgg caagcgccac gacgcggtct acgagtacga
      481 agcaggcttc gacgacagcg gccgcattct cggcgcgcgc gtggaaatcg ccttacgtgc
      541 cggttattcc gccgacctgt cgggcgcggt cgccactcgc gcggtctgcc acttcgacaa
      601 cgcgtactac ctgtccgacg tcgatatcgt cgcgctgtgc tgcaagacga atacgcagtc
      661 gaataccgcg tttcgcggtt tcggcggccc gcaaggcgcg ctggtgatgg aagtgatgct
      721 cgacagcatc gcgcgtcaat tgaagtgtga tccgctcgac gtgcgcctcg cgaattacta
      781 cggcatcggc gagcgcgaca cgacgccgta cggacaacgc gtcgaagaca acatcatcgc
      841 gccgctcacc gacgcgttgc tgaaaaccag cgactatcgc gcgcgccgcg cagcaatcgc
      901 cgcgttcaat gggaaaagcc cggtgctcaa acgcggcctc gcgttctcgc cggtgaagtt
      961 cggcatttcg ttcaacgtgc ctttcctgaa tcaggccggc gcgctggtgc atgtgtacaa
     1021 agacggttcg gtgctcgtca atcatggcgg taccgaaatg ggccaggggc tcaatacgaa
     1081 ggtcgcgcaa gtcgtcgcta atcagttcgg cttgccgctt tcgcgcgtgc gcgtgacagc
     1141 gaccgatacg tcgaagatcg ccaacacctc ggcaaccgcc gcctccaccg gcagcgacct
     1201 gaacggcaaa gccgccgaag ccgccgcgcg gaccattcgc aacaggctcg ccgaactagc
     1261 cgcgaaacaa ctcggcggca ccgccgacgc ggtggaattc acccacggtg aagtatcggt
     1321 aaacggcggt gcaatgccgt tcgaacaact ggtcggcgca gcgtatctgg cgcgcgtgca
     1381 gttgtggtcg gatggtttct atacgacgcc gaaagtgcac tgggatgcga aaacgctgac
     1441 cggtcacccg ttttactact ttgcgtacgg cgcggcggtg tcggaagtgg tgatcgacac
     1501 gctgaccggc gaatggaaac tggtgcgcgc ggacgtgttg cacgacgccg gtcaatcgat
     1561 caatccggcg atcgatatcg gccaggtgga gggcggtttc attcagggca tgggctggct
     1621 caccacggaa gaattgtggt ggaaccgcga tggccgtctg atgacgcacg caccgtcgac
     1681 gtacaagatt cctgccgtga gcgatacgcc cgcggcgttc cacgttcagc tctaccagaa
     1741 tcagaacgcc gagccgaccg tgttccattc gaaagccgtg ggcgagccgc ctctgctgct
     1801 gccgttctcg gtgttcctcg cgattcgtga cgcggtcgcg gctgcggtgc cgggcgcgga
     1861 acatgcaccc gcgttgcgcg cgccagccac gcccgaagcg attctcgatg cgctggacgc
     1921 gctgcaaccg cctgggacga cgatgcaacc cgctacagaa caaccagcga aacagccaac
     1981 ggaagcggcc gtcacggcca caaccgtcac cacccctgtt tagcaacgtg acgaaacgtt
     2041 gaagaacgtt aagcaacatc aagcaaaggc ccggcgcgac acgaacgcgt cccgccacac
     2101 tcaaccggcc gcacggacac cccgcgcggc cacgtatgga gaaccgccgg atgcaagcct
     2161 ggctacctga cctgcaacaa ttgctcgcgc acggcgacgc cgccgtgctg gtaacggtcg
     2221 cgcgcgtcga aggttcggcg ccccgcgaag ccggcaccaa gatgatcgtc acgcgcgatt
     2281 cggccaaaca cacgattggc ggcggacacc tcgaatggaa ggccatcgag accgcacgcc
     2341 aggtgctgcg cgacggcatg cgctcgccgc atatgcgccg cctcgaacgt ttcgcgctgg
     2401 ggcccagcct cggccaatgc tgcggcggcg ccgtgattct cgccttcgag cggctcgaca
     2461 tcggcgacct cggctggatc acgtcgcttg cgaaacgcgt ggccgcgggc caatcgacgg
     2521 tgcgtagcgt atcattcggc cccgcaccgg atgcggtgat gctgtccgac cccgagccgg
     2581 gtgtggatgc ctccgactgc ctgctgtggg acggcgccgg tttcgacgac agcagcgcgc
     2641 tgctcaccga aaccatcgcg ccgggcgact tcgccgtcgt gctgttcggc gccggccacg
     2701 tgggcgcagc gctagtccgg gtgctggcca cgctg
//
LOCUS       DATASH010000750         6696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2497798,
            whole genome shotgun sequence.
ACCESSION   DATASH010000750 DATASH010000000
VERSION     DATASH010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6696
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2497798"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(298..1314)
                     /locus_tag="VHV99_20015"
     CDS             complement(298..1314)
                     /locus_tag="VHV99_20015"
                     /EC_number="3.5.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010099870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008660 -
                     1-aminocyclopropane-1-carboxylate deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0042217 - 1-aminocyclopropane-1-carboxylate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-aminocyclopropane-1-carboxylate deaminase"
                     /protein_id="HEX3637727.1"
                     /translation="MNLQRFPRYPLTFGPTPIQPLKRLSDHLGGKVHLYAKREDCNSG
                     FAFGGNKTRKLEYLIPEALAQGCDTLVSIGGIQSNQTRQVAAVAAHLGMKCVLVQENW
                     VNYSDAVYDRVGNIQMSRILGADVRLVADGFDIGFRKSWEDALESVRAAGGKPYAIPA
                     GCSDHPLGGLGFVGFAEEVRRQEADLGFKFDYIVVCSVTGSTQAGMVVGFAADGRAER
                     VIGIDASAKPGQTREQITRIAKQTAEKVGLGRDITDKDVVLDERYGGPEYGLPNAGTL
                     EAIRLCARLEGVLTDPVYEGKSMHGMLDMVRNGEFPEGSRVLYAHLGGVPALNGYSFI
                     FRNG"
     gene            1597..2052
                     /locus_tag="VHV99_20020"
     CDS             1597..2052
                     /locus_tag="VHV99_20020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEX3637728.1"
                     /translation="MLDRIDRAILRQLQTDASISNVALAAKVKLSAPACLRRVERLKE
                     SGLIRGVVALIDPKAAGAGMLVIIGVVLDRSTPESFAEFEKAAQKVAGCMECHVVTGE
                     FDYFMTIRTRDSDSFNRLHAEQLLYLPGVRQIRSFMVLKEILSTTKFPL"
     gene            2187..2699
                     /gene="msrB"
                     /locus_tag="VHV99_20025"
     CDS             2187..2699
                     /gene="msrB"
                     /locus_tag="VHV99_20025"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017425457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX3637729.1"
                     /translation="MTCSRRLFFRLIGAASLAGSTAAWRTASAADMGAAAAYEIAHSD
                     AEWHKLLTDAQYRVLREAGTERPFSSPLNDEHRAGTFACAGCALPLFSSKTKFDSHTG
                     WPSFWQPLDHAVITHTDKSFGMTRDEVLCRRCGGHLGHVFDDGPKPTGLRYCMNGLAL
                     TFTPAAPGTS"
     gene            2719..3462
                     /gene="msrA"
                     /locus_tag="VHV99_20030"
     CDS             2719..3462
                     /gene="msrA"
                     /locus_tag="VHV99_20030"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014971830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX3637730.1"
                     /translation="MNIEKRIIGKPARSRVGAVALSVGLGALALLLAQRIAFSSETGV
                     VIAPPALDEPIPATAAHEETAVFAGGCFWGVQGVFQHVRGVTKAVSGYSGGQRGTAQY
                     ETVSGGDTGHAESVQVTFDPTKVTYGQLLQVYFSVIQDPTELNRQGPDSGTQYRSAVF
                     PLNDTQRHVAQSYIAQLEKAHAFPAPIVTKTEPFKGFYPAEFYHQNYLTLHPNSAYIA
                     LYDIPKVANLKRLFPNLYRDKPVLVEAAQ"
     gene            3984..4880
                     /locus_tag="VHV99_20035"
     CDS             3984..4880
                     /locus_tag="VHV99_20035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006046992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HEX3637731.1"
                     /translation="MLNSRRKRIGKVRNKQTSIAATSLIAATIGGMAVSPDSALAQTP
                     SPLAEWQYSVGIPLQKMWQPTVPDWQVRVGAATSFQPRYEGSDRYHVMAGPSIDVRYK
                     DLFFFSSGEGLGVNFAQGQNWRASLAAVYDLGRRAHDDAQELNGLGNINPAPGLKLAG
                     EYVVSKDFPLVLRADVRRYFGGSNGWIGDLGAYMPMPGSTEKFFWFAGPNVSFADSNY
                     MNSWYGVNQNQAAHSQYSQYHASAGFKSVGFGISAVWLFDKHWFVTADGAFEQLVGGA
                     GNSPLAHTRATGVADISINYRF"
     gene            5069..>6696
                     /gene="katE"
                     /locus_tag="VHV99_20040"
     CDS             5069..>6696
                     /gene="katE"
                     /locus_tag="VHV99_20040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HEX3637732.1"
                     /translation="MAGKTNTQKPAAKPAQANDPKSQDLEQFRVSPDGQALRTNQGVK
                     IADNQNTLRAGPRGPSLLEDFIMREKITHFDHERIPERIVHARGSAAHGVFQVYESMR
                     EYTKAAFLQDPNEQTPVYVRFSTVQGPRGSADTVRDVRGFAVKFYTQEGNYDLVGNNM
                     PVFFIQDAIKFPDFVHAVKPEAPNEMPTGGSAHDTFWDFVSLVPESTHMVLWTMSDRA
                     IPRSLRTMEGFGIHTFRFVNAEGKARFVKFHWRPVLGSYSLLWDEAQKLAGKDPDFHR
                     RDLWEAIERGDFPEFELGVQIVEEADEHKFDFDLLDPTKLIPEERVPVKIIGKMTLNR
                     NPDNFFAETEQVAFHPGHIVPGLDFSNDPLLQGRLFSYTDTQISRLGGPNFHEIPINR
                     PVCPFNNNQRDAMHRQAINVGQASYEPNSVSGGWPKETDPAPADGGFESYQERVEGTK
                     IRVRSESFADHFSQAALFYNSMSQPEKDHIAAAYQFELGKVTKPEIRARVVNEILANF
                     DAGLAAKVAEGLGLPAPKKGTAKLKGTKASPALSL"
BASE COUNT         1356 a         2048 c         1997 g         1295 t
ORIGIN      
        1 agtgcgtcct tctgctccgg tgtcagatcc ttgatgtcgg gcatcttcgt catatccctc
       61 actatgcaca gcctggtagc agtttacaag cgctgaatag ttacgccgcc gcaaagaaag
      121 ataagcaaag aaagcggctc aacccgccag ctcataagcg gggcccccgc gcagtcgcgg
      181 tagtggtgca tctggaatcc gtgttctcgc acattcggcg cgagtgacaa agggctcata
      241 cttcccgact cgcactgcgt gctcgccgga atgggtatcg ggaagactgg ttgctgctta
      301 gccgttccgg aaaatgaagc tgtagccgtt cagcgccggt acgccgccca ggtgcgcgta
      361 taacacgcgt gagccttccg ggaattcgcc gtttcttacc atgtcgagca tgccgtgcat
      421 cgatttgccc tcgtataccg ggtcggttaa tactccctcc aaccgcgcgc acagacggat
      481 tgcctccaat gtccccgcgt tcggcaaacc gtactccgga ccgccatagc gctcgtccaa
      541 caccacatcc ttgtccgtga tgtcgcggcc aaggccgact ttttccgctg tctgtttcgc
      601 gattcgggtg atctgctcgc gcgtttgccc cggtttcgct gacgcgtcga tgccaatcac
      661 gcgctcggca cggccgtcgg cagcgaaacc caccaccatg ccggcttgcg tgctgcccgt
      721 gacagagcac accacaatgt agtcgaactt gaagcccaag tcggcttcct gtcggcgcac
      781 ctcctcggcg aaacccacga aacccaggcc gcccagcgga tggtcggaac agcccgccgg
      841 aatcgcatac ggtttgccgc ccgcagcccg cacgctctcc aatgcgtcct cccaactctt
      901 gcgaaaacca atgtcaaagc cgtcggcgac caaccggacg tctgcgccca gaatgcgcga
      961 catctgaatg ttgccgacgc ggtcgtacac ggcgtccgaa tagttcaccc agttctcctg
     1021 caccagcacg cacttcatcc ccagatgcgc agcaacagcc gccacctgac gggtctggtt
     1081 cgactggatc ccgccaatcg acaccagcgt gtcacaaccc tgcgccaacg cctcgggaat
     1141 cagatactca agcttgcgcg tcttgttgcc gccaaacgca aagccgctgt tgcagtcctc
     1201 gcgcttcgca tacagatgca ccttaccgcc cagatggtcg ctcaaccgct tcaacggctg
     1261 gatcggcgtc ggcccaaaag tcagcgggta gcgagggaat cgttgcaggt tcatggcagc
     1321 tccgagttgt agattcgacg cgaccaggaa gtcgtcgcgc taaacaaatg gtagggaaaa
     1381 gcgatagaaa tgagcttgca aagaaagtcc attcggcata atttttaaac ttaatgatta
     1441 ccaacggctt aataaaaggc cgaatggaag ccaatgaacg caataaaagt tcgtgctatc
     1501 ccggcggcgg tacacggaaa gaccggaaag gcagagagag ccgcaagagc cgcagcgaag
     1561 gtagcaaccg caactgccga agccgatccg cttcacgtgc tcgatcgcat cgaccgggcg
     1621 atcctgcgtc agttgcaaac cgatgcgtcg atttcgaatg tcgcgctcgc ggccaaagtc
     1681 aaactgagcg cgccggcgtg tttgcggcgc gtcgagcggc tgaaggagtc agggctgatc
     1741 cgcggcgtcg tcgcgttgat cgacccgaag gcggctgggg cgggcatgct ggtcatcatc
     1801 ggcgtggtgc tggatcgatc cacgccggag tcgtttgccg agttcgagaa agcggcgcag
     1861 aaagtggcgg ggtgcatgga gtgccacgtc gtgaccggcg agttcgacta tttcatgacg
     1921 atccgcacac gcgatagcga cagcttcaat cgtctgcacg cagagcagtt gctgtatctg
     1981 ccgggcgtcc ggcaaatccg ttcattcatg gtgctcaagg aaatcctgtc gaccacgaaa
     2041 tttccgttgt aaccgccgcc gtcacataaa aagacgcgca tgctgtcata acgctccctg
     2101 ctggccgact aaatggcgag accactcgca ggcgtcgcat cagcgcctga acgggtgaac
     2161 ggtgcggact ttccggaacg atcatcatga cttgcagcag acggctattc ttcaggttaa
     2221 tcggcgcggc ctcacttgcc ggcagtaccg ccgcctggcg cacggcaagc gcggcggata
     2281 tgggcgcggc ggcggcctac gagattgcgc acagcgacgc cgaatggcac aagctgctca
     2341 cggacgcgca ataccgcgtg ctgcgcgaag ctggcacgga gcggccgttc agcagcccgc
     2401 taaacgacga gcaccgtgcc ggcacattcg cgtgcgcggg ttgtgcattg ccgctgtttt
     2461 cgtcgaagac gaagttcgat agccacaccg gctggccgag tttctggcag ccgttggatc
     2521 acgcggtgat cacgcacacc gacaaatcct tcggcatgac tcgcgacgaa gtgctgtgcc
     2581 ggcgttgcgg cggccatctg ggtcacgtat tcgacgacgg ccccaagccg accggtctgc
     2641 gctattgcat gaacggcctc gctttaactt ttacgcccgc cgcgcccggc acgagctgat
     2701 cgaatcagga cggacatcat gaacatcgag aaacgcatca tcggcaaacc ggcccgctcg
     2761 cgtgtgggcg ctgtcgcgct ttcggtgggg ctcggcgcgc tggcgctgct gctggcgcag
     2821 cgcatcgcgt tttcatcgga gaccggagtt gtcattgcgc cgcccgcgct cgatgagccg
     2881 atcccggcca cggccgcgca cgaagaaacc gccgtgttcg cgggcggctg cttctggggc
     2941 gtgcagggcg tgttccagca cgtgcggggc gtcacgaagg cggtgtcggg ttattccggc
     3001 ggccagcgcg gcaccgcgca gtacgagacc gtcagtggcg gcgacaccgg ccatgctgaa
     3061 tccgtgcagg tcacgttcga tccgaccaaa gtcacttacg ggcaattgtt gcaggtctat
     3121 ttctcggtga ttcaggatcc aaccgagttg aaccgccagg ggccggatag cggcacgcaa
     3181 taccgttcag cggtgttccc gctgaatgac acgcagcgcc acgtcgcaca aagctatatc
     3241 gcacaactcg aaaaggcaca cgctttcccc gcgccgatcg tcacgaagac ggagccgttc
     3301 aaaggcttct acccggctga gttctaccat cagaactacc tgacgctgca tccaaactcc
     3361 gcgtatatcg cactctacga catcccgaag gtcgcaaacc tgaagcggct gtttccgaat
     3421 ctctatcgcg acaagcctgt gctggtcgaa gcggcgcagt aagacgcgag cgccgcgtca
     3481 gcggcaaact gacgggtccg gcaccgcgcc tgcgcggcag cgggcatcgc tcgacgatgt
     3541 atcgcgagac gcgttcgccc agcgcgtcga acaggaccgg gcggttctcg ccgtgcgaat
     3601 ggacgatcgc tgcctgcaac acggcggccg ccgcccaatt tttttgcttg tattctgcca
     3661 gtcattgtcg ccacggcgtg cttgccctca ccgttggtcc gtcctcaggt tttactatcc
     3721 aggccgatgc tccagcgacc aggcccgcat tttgcactgg accctgcgtc ggcaatcttt
     3781 cgcacgtcac tgttcataca aaagcccaat ttacggggtg gcatcagctt tgaacagtgc
     3841 gttttgcccg gcaatgcgtg tcgtctgctg aaaaaattac acgtcgtgcg tagcagtgcg
     3901 gcggtcgtgc cggagttttt taatccatct ggatttcggg cgagaacata aaccccttcg
     3961 cgatacgcga gaacggaggc tctgtgttga acagccgtag aaagcggatc ggcaaggtcc
     4021 gcaataaaca aacaagcatc gcggcaactt ctctaatcgc cgcgaccatc ggcggtatgg
     4081 ccgtttcacc ggattcggcg ctggcccaga cgccgtcgcc cttggccgaa tggcagtatt
     4141 ccgtcggcat tccgctgcag aagatgtggc agccgactgt cccggactgg caggtgcgcg
     4201 tcggcgcggc gacgtcgttc cagccgcgct atgagggctc cgaccggtat cacgtgatgg
     4261 ccggtccgag catcgacgtg cgctacaagg acctgttctt cttttcgagc ggcgagggcc
     4321 tcggtgtcaa cttcgcgcag ggacagaact ggcgcgcgag tctcgcggcg gtctacgacc
     4381 tcggacgtcg cgctcacgac gatgcccagg aactgaacgg cctcggcaac atcaatcccg
     4441 cgccgggcct caaactcgcc ggcgaatatg tcgtctcgaa ggatttcccg cttgtgttgc
     4501 gcgccgacgt gcgccgctac ttcggcggat cgaacggctg gatcggcgat ctgggcgctt
     4561 atatgccgat gcccggcagc acggagaaat tcttctggtt tgccggtcca aatgtatcgt
     4621 tcgccgactc caactacatg aatagctggt acggcgtgaa tcagaatcag gcggcgcact
     4681 cgcaatactc gcagtatcac gcgagtgccg gcttcaagtc ggtgggcttc ggcattagcg
     4741 cagtgtggct gttcgacaag cactggtttg tcactgcgga cggcgcattt gaacaactgg
     4801 tcggcggtgc gggcaatagt ccgcttgccc acacacgcgc caccggtgtg gccgatatct
     4861 ccatcaacta tcgcttctga cacagcgttt tcgtgtctga gcgtgtggga caaaggcgtg
     4921 tcattgcggc acgcctcagt gaagtgcggt ggaggattcg cgggggaccc tacgcaggtt
     4981 aattcgcggg tacatcgttt gctgctttct tctgatgcgt taaaccggat atttccccgg
     5041 tgcgcccgaa tgcaaggagc aaatccccat ggcaggtaaa acgaatacgc agaagcccgc
     5101 agcgaagcct gcgcaggcca acgatccgaa atcgcaggac ctcgagcagt ttcgagtctc
     5161 tccggacggc caggcgctga gaaccaacca gggcgtgaaa atcgccgaca accagaacac
     5221 cctgcgcgcg ggtccgcgcg gcccctcgct gctggaagat ttcatcatgc gtgagaagat
     5281 cacgcacttc gaccacgagc ggattcctga gcgcattgtg catgcacgcg gctcggcggc
     5341 gcatggcgtg tttcaggtgt acgagtcgat gcgcgagtac accaaggccg ccttcctgca
     5401 ggacccgaac gagcagacgc cggtctatgt acgtttctcg acggtgcagg gcccgcgcgg
     5461 ctcggccgat acggtgcgcg acgtgcgcgg cttcgcggtg aagttctaca cgcaggaagg
     5521 taattacgat ctggtcggca acaacatgcc ggtgttcttc attcaggacg ccatcaagtt
     5581 tcccgacttc gtgcatgcgg taaaaccgga ggcgccgaat gaaatgccga ccggcggctc
     5641 cgcgcacgat accttctggg acttcgtttc gctcgtgccc gagtccacgc atatggtgct
     5701 gtggaccatg tcggaccgcg cgattccgcg cagcctgcgc accatggaag gcttcggcat
     5761 tcacacgttc cgcttcgtga atgcagaagg caaggcgcgc ttcgtcaaat ttcactggcg
     5821 tccggtgctg ggctcgtatt cgctgctgtg ggacgaggcg cagaaactcg ccggcaaaga
     5881 cccggatttc catcggcgtg acctgtggga agcgatcgaa cggggcgact tccccgaatt
     5941 cgagctgggc gtgcagatcg tcgaggaagc tgacgagcat aagttcgatt tcgacctgct
     6001 cgatccgacc aaactgattc cggaagaacg ggtgccggtg aagatcatcg gcaagatgac
     6061 gctcaatcgt aatccggaca acttcttcgc cgagaccgaa caggtcgcat tccatccggg
     6121 ccacatcgtg ccgggtctgg atttctcgaa cgatccgcta ttgcaagggc gtttgttctc
     6181 gtacaccgac acgcagatca gccgccttgg cggaccgaac ttccacgaga ttccgatcaa
     6241 ccgcccggta tgcccgttca acaacaatca gcgcgatgca atgcatcgcc aggcgatcaa
     6301 tgtcgggcaa gcctcgtatg agccgaattc ggtgagcggc ggctggccga aggaaacaga
     6361 tcccgcgcct gcggatgggg gattcgaaag ctatcaggag cgcgtggaag gcaccaaaat
     6421 tcgtgtgcgc agcgagtcgt ttgccgatca tttttcgcag gccgcgctgt tttataacag
     6481 catgtcccag cctgagaaag atcatatcgc cgcggcgtat cagttcgaac tcggcaaggt
     6541 caccaagccg gagattcgcg cgcgcgtggt caacgagatc ctggctaatt tcgatgctgg
     6601 gcttgccgcg aaggtggccg aaggtctcgg tttgccggcg ccgaagaagg gcacggcgaa
     6661 gctcaagggg actaaagcgt cgcccgctct gagtct
//
LOCUS       DATASH010000751         2905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6254055,
            whole genome shotgun sequence.
ACCESSION   DATASH010000751 DATASH010000000
VERSION     DATASH010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6254055"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="VHV99_20045"
     CDS             complement(<1..993)
                     /locus_tag="VHV99_20045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013342953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX3637733.1"
                     /translation="MTWTVDEQLALTATEAVAAIQSGRLKAADYMATLLARAAALSSL
                     NALTTLDLDGALAAAQRIDALPPEAKARLALAGLPIVVKDNINTAGLQTSAGTPALEG
                     FVPKTSAPSVQRLLDAGAIVLGKANMHELAFGITSTNLAVHAGPVRNPYDPSLIPGGS
                     SGGTAVAIAARIVPAGLGTDTGGSTRIPAALTGTAGFRPSVGNGGAERRYHDPNAVVP
                     ISHTRDTVGPMARTVADIALLDGVITGAGPLPALALSGLRIGLPAPLWEGLERQVEDV
                     ARAALRRLEAAGVIFVPVAMSELESLNGRVGGPLAIHEPREDVLAWLVANDAPVR"
     gene            complement(1186..1401)
                     /locus_tag="VHV99_20050"
     CDS             complement(1186..1401)
                     /locus_tag="VHV99_20050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006046660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX3637734.1"
                     /translation="MNSNIRYKGYEVAPAAQRLPNGLFAANLTIEKTTTSPGQAYSFD
                     ALDYFFDEELALAYAFSWGRMWIDNRQ"
     gene            complement(1766..2425)
                     /locus_tag="VHV99_20055"
     CDS             complement(1766..2425)
                     /locus_tag="VHV99_20055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX3637735.1"
                     /translation="MTSFDHLRRPLVIGIGGTTRAASSTERALGFALRGAQAAGAETR
                     LFGGTFLHSLPHYAPENPQRTAEQLELIEAVRHADALVIATPGYHGGVSGLVKNALDT
                     LEELRADERPYLDGRAVGCIVTAYGWQAAGSVLTSLRSIVHALRGWPTPFGAGINTLE
                     TRFDTVDTCSDAKVVDQLATVGQQAAQFALAFNAHRTPSAAPLAPAVKEAQPARLLHA
                     V"
BASE COUNT          482 a          868 c         1031 g          524 t
ORIGIN      
        1 cctgacgggc gcatcgttgg cgacgagcca ggcgagcacg tcctcgcgcg gctcgtggat
       61 cgcgagcggg ccgcctaccc ggccgttcaa gctttccagc tcgctcatcg cgacgggcac
      121 gaagatgacg cccgcggctt cgagccgacg caaggcggcg cgcgcgacgt cttccacctg
      181 cctttcgagt ccttcccaga gcggtgcggg caagccgata cgcaagccgc tcagcgcgag
      241 cgcgggcagc ggacccgcac cggtgatcac gccgtcgagc aaggcaatat cggcgaccgt
      301 gcgcgccatc ggacctaccg tgtcgcgtgt atggctgatc ggcacgacag cattcggatc
      361 gtgatagcgc cgctcggcgc cgccgttgcc cacggacgga cgaaaccccg cggtgcccgt
      421 caacgcggcc gggatgcggg tggagccgcc ggtgtcggtg ccgagaccgg cgggcacgat
      481 gcgcgcggcg atcgccacgg cagtgccccc cgacgagccg ccggggatca gcgaggggtc
      541 gtacggattg cgcaccggcc cggcgtgcac ggccaggttc gtgctggtga tgccgaacgc
      601 cagctcgtgc atgttggctt tgccgagcac gatcgcaccg gcatcgagca gccgttgtac
      661 cgagggcgcg cttgtcttcg gcacgaagcc ctcgagagcg ggcgtgcccg ccgaggtctg
      721 caagccggcc gtattgatgt tgtcctttac cacgatgggc aaaccggcga gcgctaagcg
      781 tgctttcgct tcgggcggta acgcatcgat gcgctgcgcg gcggcgagcg cgccgtcgag
      841 atcgagcgtg gtgagcgcat tgaggctcga gagggctgcg gcgcgcgcca gcagcgtggc
      901 catatagtcg gcggccttga gacgccccga ctggatcgcg gcgaccgcct ccgtggcggt
      961 tagcgccaac tgttcatcga cggtccatgt cattgccggc gtctccttga actgaagtgt
     1021 cgcctattct ggcacagccg cttaaaaatc gacgtcaggt gaggaaagat ggcctgggtt
     1081 tacaggggag tccgttgccg ctgcgcaggg caaccggttt ggtgggtggt gcgcggttga
     1141 ccggcgcgag cggccgccaa agtgcttcgg gcggcccggt tactgttact gccggttgtc
     1201 gatccacatg cgtccccagc tgaacgcata ggcaagcgcg agctcttcgt cgaagaagta
     1261 atctagcgcg tcgaacgagt aggcctggcc cgggcttgtg gtggttttct cgatggtcag
     1321 gttggcggcg aacaggccgt tgggcagccg ttgcgcagcc ggtgcgactt cgtaaccctt
     1381 gtagcggata tttgaattca tgatcgtggc agtaagtgtg cccgcgtgtg ttgcaaagga
     1441 gccggcacgc gcgacgcaac cctgtgcgtc aggcaaccgg tggtgagcgg gcagatcaag
     1501 cgtaagccgc gccgcgcagc gggtgaacca atctttcgtc gtaagcaaat cagcgaggtt
     1561 cggcaacggt cttcgggagg cctgatcgga gcatagtcgc tggatgtgac aacactatcc
     1621 ggtcttgtcg acggatggtc tgttatgtgg cgcacggttt gaagcggggc ggcgcggccg
     1681 cgtcgacgaa aaaacggtgg gtaaggaaga ttgtggagcg ttcgtggacc gcaagccgcg
     1741 gtccacgatg ccatgcggtt cgcgcttaca cggcgtgcag caggcgggca ggttgtgctt
     1801 ccttgactgc gggggcaagc ggcgcggcgc tgggcgtgcg atgcgcattg aacgccaggg
     1861 cgaactgggc ggcctgctgg ccgaccgtgg cgagctgatc gaccaccttg gcgtcggaac
     1921 aggtgtcgac ggtgtcgaaa cgcgtttcca gcgtattgat cccggcgccg aacggcgtag
     1981 gccagccgcg cagcgcgtga acgatcgagc gcagcgacgt cagcaccgag ccggcagcct
     2041 gccagccata agcggtaacg atacagccga ccgcgcggcc atccagatag gggcgctcgt
     2101 cggcgcgcag ttcttcgagc gtgtcgagcg cgttcttgac gaggccggag acgccgccgt
     2161 gatagccggg cgttgcgatg accagcgcgt ccgcgtggcg cacggcttcg atcagttcga
     2221 gctgttcggc cgtgcgttgc gggttttccg gcgcgtagtg gggcaggcta tgcaggaagg
     2281 tgccgccgaa caggcgggtc tccgcgcccg cggcttgcgc gccgcgcaac gcaaagccga
     2341 gcgcgcgctc ggtcgacgac gccgcgcgtg tcgtgccgcc aatgccgata acgagtggcc
     2401 ggcggagatg gtcgaaactg gtcaacgaaa tgctccttcg gtagcggctc acggggcgct
     2461 ggcgcggggc tgcgcgcaga cgtaccgggg cgaaccggac tttgaagtgt catcttaatg
     2521 atcgccgcga ccacggcgaa gcgacatttt gttctaacga tataaccgcg cggagttatc
     2581 ggcgccccaa cgggctgtct gacggcagtt tcgaagcaga ttctccggcc ctgtgcaatg
     2641 cggttgaagc cacgcgggat aagcatatgg cgaaccattg tttgggggcg cgatgacgcg
     2701 cggctatgat tgagctgtct cctccatgac atctccttga catgggactc ggcctcgcta
     2761 cgccagtggt gaggcttttt ttttgggggg tctttgtccg gtctttcggt cgcgtcagcg
     2821 cttgctgctt cacgataaag ccgttttttg catgctacga tggtccgcca gtgattcccg
     2881 gcgaggtcga gcaagcgatg gcaac
//
LOCUS       DATASH010000752         6895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4477397,
            whole genome shotgun sequence.
ACCESSION   DATASH010000752 DATASH010000000
VERSION     DATASH010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6895
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4477397"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            951..1769
                     /locus_tag="VHV99_20060"
     CDS             951..1769
                     /locus_tag="VHV99_20060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX3637736.1"
                     /translation="MRSWRISTPPRTGQINTSRVTDLVMSISHADPDALAASILKTVG
                     DTLPISQCTIFAYEFDARPRTVSVADHRGGRFLRDVAERYATHFHALDGNRAVIGRPP
                     TKQPHQALVLHQQHSEEIENEAYRAACYAQPNVSDRLSLLLQPVESVWLSINLYRDSA
                     YGMFQPGELEHVEDLAHLFAHAAKSHYAINGQYQQSTAAAMLARVRRACPALTPREID
                     VLRGTLEGANAAEIADQMGIKATSVITYQKRAYARLGISSQRQLFALCLLPERS"
     gene            complement(1743..2711)
                     /locus_tag="VHV99_20065"
     CDS             complement(1743..2711)
                     /locus_tag="VHV99_20065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3637737.1"
                     /translation="MPLTRVTIRQFEAFLAIVDLHSIGAAAERLGLSSSAVSQLLAEL
                     ETELGFRLFDRTTRRVNLSSAGQDFLSSAESVLRYVRAAAQSADDIRNRAAGIVRVGA
                     PLVLAATALPAAIAEYARDKPKVVVRIVDTVVEQLVERVESGDVDLAIGPDRQSGTRV
                     RCDPAFASPWVMWCAPAHPLSARRRVQWQHVRDVPIVATGRDHERAVEQMLANLPVEA
                     RIIPVDVVDNVTTAFGVASQGLAVTLAPAYVAPLARTFGLVMKRIVDPETIREVCVYR
                     STERALSPPADGFAEFIMPWLKRWDRETQADGRTKRAGSRPLRQQT"
     gene            2940..4139
                     /locus_tag="VHV99_20070"
     CDS             2940..4139
                     /locus_tag="VHV99_20070"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HEX3637738.1"
                     /translation="MFDHIPAYAGDPILSLNEDFQLDPRPNKVNLSIGIYFDDAGTLP
                     VMDAVRRAETALLDSIGPRSYLPMAGLPLYRDAAQALVFGADHEARAAGRIATLQTIG
                     GSGALKVGGDFLKRYFPGSQIWISDPSWENHRVVFEGAGLTVNTYPYYDEYTGGLRFA
                     DMVETIDRLPEQSIVLLHACCHNPTGVDLSPAQWAELVPVLQRRKLIAFVDMAYQGFG
                     AGLEEDAACVRLLADAGVPLIAANSFSKNFSLYGERCGALSVVCKSKEEAARVLGQLM
                     STVRSNYSNPPTHGARLVANVLSDTSLRASWAAELATMRERILAMRGTIHAGLAGRVD
                     EVMRARYVAQRGMFTYTGLSEAQVERLRSEYAVYVLRSGRMCVAGLNARNAGYVASSI
                     AAVVAGT"
     gene            4244..5035
                     /locus_tag="VHV99_20075"
     CDS             4244..5035
                     /locus_tag="VHV99_20075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX3637739.1"
                     /translation="MTTLEAQPASPPKSAAMHPDEWQARVQLAAAYRIFDMLGWTEMI
                     YNHITLRVPAIVNGGTRHFLINPFGLHYSEVTASNLVKIDAQGRVLDNSPYPVNPAGF
                     VVHAAIHEGLADAHCVMHTHTTAGVAVACLENGLEQTNFYSAQLHDRIAYHDFEGITV
                     HAEEGPRLLEHIGNRQAVILRNHGLLAWGHTLPQTFAILWTLNRACEIQMATFSMGRA
                     RPVPEEVAIRCSRDSLQFDPRHGAGQDVFDALVRRVDRIDASYKD"
     gene            5139..6122
                     /locus_tag="VHV99_20080"
     CDS             5139..6122
                     /locus_tag="VHV99_20080"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX3637740.1"
                     /translation="MKAGIYGAGAIGGWIGVKLAQAGHDVSVVARGATLDALQQHGLR
                     LIEGGVTHTATVNASEQPADLGAQDLVVVAVKGPAMASVAAHIAPLLNSRTIVLTAMN
                     GVPWWFCDGLGRDFASQRLKSIDPEGAIASAIPTAQTVGCVVHASCLVEAPGVIKHHQ
                     GNGLIIGEASGQPSERVKALTATFAAAGFNATVSEQIQRDVWYKLWGNMTMNPVSAIT
                     GATTDRILSDELVRNFVTSIMLEAKEIGTRFGIPIEQAPDDRNEVTLKLGAMKTSMLQ
                     DVQAGKQVELDALVAAVRELGQLTDVPTPYTDALLGLARLHASTLGLYPRQ"
     gene            complement(6133..6324)
                     /locus_tag="VHV99_20085"
     CDS             complement(6133..6324)
                     /locus_tag="VHV99_20085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637741.1"
                     /translation="MTDSELDLVYTTLCKTLTAEGEAQAPLYLARLALLSMTEIGDTQ
                     RALSLIEAARLPASAAVTA"
     gene            complement(6344..>6895)
                     /locus_tag="VHV99_20090"
     CDS             complement(6344..>6895)
                     /locus_tag="VHV99_20090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3637742.1"
                     /translation="LTDVPTPYTDALLGLARLHASTLGLYPRQTHAIAYAKSPLNWIA
                     AVASGTFSKGPAPGTVLPECRLQRFCEGAAVDIHLTDLLGPCFTALRFGADVAQDEAW
                     QQLHHALGEHSIPFKAVTLITGDAPRPASTHVVDPAGRLHEMLDAKPGTVYLIRPDGH
                     VLARWRNGNAANVQSAVTASLTN"
BASE COUNT         1381 a         2204 c         2099 g         1211 t
ORIGIN      
        1 gaatcgggca tgcaagcgtt ctgtacgatc cgctcctacc ttgccacctt gcgtaagcag
       61 aaccattcgc tgattgatgc actggcctcc agctttgccg gaatcgtcgt ttcgcctttg
      121 tctgccgctg aatagttacg cctgccgcaa agaaaagtag gcaaaagaaa gcggctcaaa
      181 ccgccagcgc ataagcgggc cccccgcaca gctacggtag tggtgcatct ggaatctgtg
      241 ttctcgcaca ttcggcgtga gtgacacagc cgtcatactt ccggcgacgc tgcgcgcgcc
      301 gaagcgtagt tcataaagcc gatcacggcg gtttcgcgct cacggctcat ttcgcgcggt
      361 cgctcatttc gcgcggtggc tcggtgtata tcgttgcttc gagtttgcct ctctctcatg
      421 ctgtcgctgg ggcgcttcgc caaggcgaag ccgaagcccg ccgcccacag ccgaagccct
      481 tggttattga tgaacccttc cggcgagcac gcagtgcgag gcgggaagta tgactgcctg
      541 gtcactcaca tcgaatgcgc gagggcaccg attccagatg cgccactacc gtggctgtgc
      601 gggggacccg cttatgagct ggcggggtga gccgctttct tttgcctact tttctttgcg
      661 gcaggcaaag aaaagtaggt gcccgccccg cacaggggcg acgctaatag accaataaga
      721 aatcaaggaa aggccaacgc cataaaagca cagacaaaca agcgccgcgc aggcaaccag
      781 accaaggaga cgccaacacc ataaaaccac agacaaacaa gcgccgccca ggcaaccaga
      841 tcaaaaaaaa agcactaccc ccacagcccc cgggcaaaaa aaacccgttg tgctacgcta
      901 cccagaccca aaactcacac accggcggcg acgccgccat caccatcatc atgcgttcct
      961 ggcgcatctc caccccgccc cgcacggggc aaatcaacac ttcgcgtgtg accgacctcg
     1021 tcatgtcgat ctcacatgcc gacccggacg cgctcgctgc aagcattctg aaaaccgtcg
     1081 gcgacacact ccccatttcg caatgcacca tcttcgccta cgaattcgac gcgagaccgc
     1141 gcaccgtctc cgtcgccgac catcgcggcg gccggtttct ccgtgatgtc gccgagcgct
     1201 acgccacgca cttccatgcg ctcgacggca accgcgccgt catcggtcgc ccgccgacga
     1261 aacagccgca ccaggcattg gtattgcacc agcagcacag cgaagagatc gagaacgaag
     1321 cctaccgcgc cgcatgttac gcacagccca atgtgtccga ccggctatcg ttgcttctgc
     1381 aacccgtgga aagcgtctgg ctgtcgatca atctgtatcg tgatagcgcc tatggcatgt
     1441 ttcagccagg cgaactcgag cacgtggaag acctggcgca tctattcgca cacgccgcga
     1501 aaagccatta cgccatcaac ggccagtatc agcaaagcac ggcagccgca atgctggcac
     1561 gcgtcagacg cgcctgtcct gcgcttacgc cgcgcgagat cgacgtgctg cgcggcacgc
     1621 tcgaaggcgc caacgccgcc gagatcgccg atcagatggg tatcaaggcc accagcgtga
     1681 tcacgtatca gaagcgcgct tacgcccgct tgggtatctc cagtcagcgt cagttgtttg
     1741 cgctatgttt gctgcctgag cggtcttgat ccggcgcgct tcgtccgccc gtcggcttgc
     1801 gtctccctat cccagcgctt cagccatggc atgatgaatt ccgcgaatcc gtccgcgggc
     1861 ggcgacagtg cacgttcggt cgagcgatac acgcacactt cgcggatcgt ttcagggtcg
     1921 acgatccgct tcatcaccag cccgaaggtt cgcgcgagcg gcgcgacata ggcgggcgca
     1981 agcgtcacgg caagcccctg ggaagccacg ccgaacgccg tggtgacgtt gtccaccacg
     2041 tccaccggaa tgatgcgggc ctccaccggc agattggcca gcatctgctc cacggcgcgc
     2101 tcgtgatcgc ggccagtggc aacgatcggc acgtcgcgca catgctgcca ctgcacccgg
     2161 cgccgcgcgc tcaacggatg cgccggcgcg caccacatca cccacgggct cgcgaacgcc
     2221 ggatcgcagc gcacccgcgt gccgctctgg cgatccggac cgatcgcgag atccacgtcg
     2281 ccactttcca cgcgctccac cagttgttcg accacggtgt cgactatgcg cacaaccacc
     2341 ttcggtttgt cgcgcgcata ttcggcaata gccgccggca gcgcagtcgc cgcgagcacc
     2401 agcggcgcgc cgactcgcac gatgccggcc gcgcgattgc gaatgtcgtc ggcggattgg
     2461 gcggcggcgc ggacatagcg cagcacggat tcggccgacg acagaaaatc ctgtcccgca
     2521 ctcgacagat tgacgcgccg cgtcgtacgg tcgaacaggc gaaagcccag ttccgtttcc
     2581 agttcagcca gcaactggct gaccgccgac gagctcagcc cgagccgttc cgccgccgcg
     2641 ccgatactat gcagatcgac aatggcgaga aaggcctcga actggcgaat ggtgacgcgt
     2701 gtgagtggca tccggcgatt ctaaagaaaa acttactaat cgtgaaaaat cgattgattg
     2761 taagcgacgc gccattggcc gacactcgtc ctcatcgacg cgaaggcgga aagccgctta
     2821 gacttccgtc tcgcccacac ggctcatgca agccggatag cgcagacctg catagtccgt
     2881 attccgggcg cacgccggcc actatccagc cctatgccgg gcccactcaa ggaatcgcca
     2941 tgttcgatca cattcccgcc tacgcgggcg acccgatact gagcctgaac gaggattttc
     3001 aactcgatcc gcggcccaac aaggtcaact tgagcatcgg catctatttc gacgacgccg
     3061 gcacgttgcc cgtgatggac gccgtacggc gcgccgaaac cgcgttgctc gattcgatcg
     3121 gcccgcgctc ctacctgccg atggcgggtc tgccgctcta tcgcgacgcg gcccaggcgc
     3181 tggtcttcgg cgccgaccac gaagcgcggg cggcggggcg gatcgccacg ttgcagacca
     3241 tcggcggctc tggcgcgctg aaagtcggcg gggattttct gaagcgctat ttcccgggct
     3301 cgcagatctg gatcagcgat ccgagctggg aaaaccatcg cgtggtgttc gaaggcgcgg
     3361 gcctcacggt caacacctac ccgtattacg acgaatacac cggcggcttg cgtttcgccg
     3421 atatggtcga aacgatcgac cgcttgccgg aacaaagcat cgtgctgttg cacgcgtgct
     3481 gccacaaccc caccggcgtc gatctgagcc cggcgcaatg ggcggaactc gtgccggtat
     3541 tgcagcgccg caaactgatc gcttttgtcg acatggccta tcaaggtttc ggcgcgggtc
     3601 tggaagaaga cgccgcctgc gtgcgcctgc tcgccgacgc gggtgtgccg ttgatcgccg
     3661 ccaattcgtt ttcgaagaat ttctcgctgt acggcgagcg ctgcggtgcg ttgagcgtgg
     3721 tgtgcaagag caaggaagag gcggcacgcg tgctcggtca attgatgtcg acggtgcgct
     3781 ccaattacag caatccgccc acgcatggcg cgcgcctcgt cgcgaacgtg ctgtcggaca
     3841 cgtcgctgcg tgcatcatgg gcggcggaac tcgccaccat gcgcgaacgg attctcgcca
     3901 tgcgcggcac gattcacgca ggccttgcag gccgcgtcga tgaagtgatg cgcgcgcgtt
     3961 acgtcgcgca gcgcggcatg ttcacgtaca caggcttgag cgaagcccag gtcgagcgtt
     4021 tgcgcagcga atacgccgtg tatgtgctgc gctcgggacg tatgtgcgtc gccggattga
     4081 atgcacgcaa cgcgggctat gtggcttcgt cgatcgcggc ggtggtagcg ggcacataac
     4141 ggcagaggtt cgccatcacc agacgataac gaacttaacg acctcaacca acgtagcgaa
     4201 cttagccaac gtagcgaacg acagcgaact ataagagacc ctgatgacga cacttgaagc
     4261 gcaaccggca agtccgccga aatccgccgc gatgcatccc gacgaatggc aggcgcgcgt
     4321 gcagctcgcc gcggcctatc gcatcttcga catgctgggc tggaccgaga tgatctacaa
     4381 ccacatcacg ttgcgcgtgc cggcgatcgt gaacggcggc acgcggcact tcctgatcaa
     4441 tccattcggc ctgcactaca gtgaagtgac agcgagcaat ctggtgaaaa tcgacgcaca
     4501 agggcgtgtg ctcgacaact cgccgtatcc ggtgaatccc gcgggtttcg tcgtgcatgc
     4561 cgcgattcac gaaggcctcg ccgatgcgca ctgcgtgatg cacacgcaca ccacggccgg
     4621 cgtcgcagtg gcctgcctcg aaaacggtct ggagcaaacc aacttctata gcgcgcagtt
     4681 gcacgatcgc atcgcgtatc acgacttcga aggcatcacg gtgcatgcgg aagaaggacc
     4741 gcgtctgctc gaacatatcg gcaaccggca agcggtgatc ttgcgcaacc acggcctgct
     4801 tgcatggggc cacacgttgc cgcagacttt cgccatcttg tggacgctca accgcgcgtg
     4861 cgaaatccag atggccacgt tctcaatggg ccgcgcgcgc ccggtgccgg aagaggtggc
     4921 gatccgttgc tcgcgcgact cgttgcagtt cgatccgcgt cacggtgccg gacaggatgt
     4981 gttcgatgct ttagtgcggc gcgtggatcg catcgacgcg agctataagg attaacgcgg
     5041 caaggatcaa cgcgaaaact aacgcgaaga ttaacgcgag gatcgacgca aaccttgagg
     5101 cgaagattgc cccgggaact aacgcaagga ttgaagtgat gaaagcagga atctacggtg
     5161 caggcgcgat cggcggctgg ataggcgtga agcttgcgca ggccggtcac gacgtcagcg
     5221 tggtggcgcg cggcgccacg ctcgatgcgt tgcagcagca cggcttacgt ttgatcgaag
     5281 gcggcgtgac gcacaccgca acggtgaacg cgagcgaaca gccggcggac ctcggcgcgc
     5341 aggacctcgt cgtggtggcg gtcaagggcc cggcaatggc ctcggtggcc gcgcatatcg
     5401 cgccgctgct gaactcgcgc acgatcgtgc tgaccgccat gaacggtgtg ccatggtggt
     5461 tctgcgacgg cctcggccgt gatttcgcga gccagcgctt gaagtcgatc gatcctgaag
     5521 gggcgatcgc tagcgcgatt ccgaccgcgc agacggtggg ttgcgtcgtg cacgcgagct
     5581 gtctggtcga agcgccgggt gtcatcaagc atcatcaggg caatggcttg atcatcggcg
     5641 aggcgtcagg gcagccgagc gaacgcgtca aagcgctcac cgccacgttc gccgcagcgg
     5701 gcttcaatgc aacggtgtcg gagcagattc agcgcgacgt ctggtacaag ctatggggca
     5761 acatgacgat gaacccggtc agcgccatca ccggtgcgac caccgaccgg attctcagcg
     5821 acgaactggt acgcaacttc gtcacgagca tcatgctgga ggcgaaggag atcggcacgc
     5881 gcttcggtat tccaatcgaa caggcaccgg acgatcgcaa tgaggtcacg cttaaactcg
     5941 gcgcgatgaa aacctcgatg ctgcaggacg tgcaagcggg caagcaggtg gaactcgacg
     6001 cgctggtcgc cgcagtgcgt gagctggggc agcttaccga tgtgccgacg ccctatacgg
     6061 acgcgttgct cggcctcgca cgtctgcatg cgagcacgtt ggggttgtat ccgcggcagt
     6121 gaaggttgcg cattacgcag tcacagcggc agaggccggc agcctcgccg cctcgatcag
     6181 cgacaacgcc cgttgcgtgt cgccgatctc cgtcatggaa agcagcgcaa ggcgtgccag
     6241 atacaagggc gcctgcgctt cgccttcggc ggtcagcgtc ttgcagagcg tcgtgtagac
     6301 gaggtcgagt tcgctatcgg tcatggtttg ggttcctttt gcgtcagttc gtcagactgg
     6361 ccgttacggc gctttgcacg ttcgccgcgt tgccgttgcg ccagcgggcc agcacatgcc
     6421 catccggcct gatcagatag acggtgcccg gcttcgcgtc gagcatctcg tgcaagcggc
     6481 ccgccggatc gaccacatgc gtgctagcgg ggcgcggcgc atcgccggtg atcagcgtga
     6541 ctgctttgaa tggaatgctg tgttcgccaa gcgcgtggtg caattgctgc catgcctcgt
     6601 cttgcgccac gtccgcaccg aagcgtagtg cggtgaagca gggcccaagc agatcggtga
     6661 gatggatatc gactgctgct ccctcgcaaa accgctgcag gcggcactcc ggcagtaccg
     6721 tgccgggcgc cggccctttg gagaacgtgc ccgacgcgac cgccgcgatc cagttgagcg
     6781 gcgacttcgc atatgcgatc gcatgcgtct gccgcggata caaccccaac gtgctcgcat
     6841 gcagacgtgc aaggccgagc aacgcgtccg tatagggcgt cggcacatcg gtaag
//
LOCUS       DATASH010000753         8466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4477423,
            whole genome shotgun sequence.
ACCESSION   DATASH010000753 DATASH010000000
VERSION     DATASH010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 8466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8466
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4477423"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            370..651
                     /locus_tag="VHV99_20095"
     CDS             370..651
                     /locus_tag="VHV99_20095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX3637743.1"
                     /translation="MPTHPSTAHNSASARASSVTARLTDSQIAAEAKTRLTWDAAIPK
                     NALKVKVSHGRIKLLGELNHEQQRTAALEDVTRLFGVTAVLDHTTIKQP"
     gene            complement(654..1820)
                     /locus_tag="VHV99_20100"
     CDS             complement(654..1820)
                     /locus_tag="VHV99_20100"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HEX3637744.1"
                     /translation="METHKQTDRQTDEATILVLNSGSSSLKFGLFAHSGDDESLLLEG
                     SAEGIGRGDGSLRIKSPDGRVLVQQDQLLESQTDALQKLARVLEAQNHERPRAVGHRV
                     VHGGPHLRTHQRLTPEVRQRLQDAVHFAPLHIPPALALIDEASAIFADAQHFACFDTA
                     FHATLPPRAAQLALPRRYMEAGVIRYGFHGLSYESLVTRLGAALPRRAVFAHLGNGSS
                     LCALQDGKSVDTSMGLTPTGGVPMGTRSGDLDPGVLLYLMRVEKLDADALETLLNRQS
                     GLAGFADGESDMQALEKRAAAGDADASLALDAFATAVRKTIGAYAALLGGIDLLVFTG
                     GIGEHSEEIRKRVCEGLAFLGLSDGDPAGKVRAIHTEEEKQIARHCRGMLRGAG"
     gene            complement(1833..4226)
                     /locus_tag="VHV99_20105"
     CDS             complement(1833..4226)
                     /locus_tag="VHV99_20105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018438037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEX3637745.1"
                     /translation="MAEAPSRPTPPQTLEPDTLRKMDRYWRACNYLSAGMIYLRDNPL
                     LREPLKPEHIKNRLLGHWGSDPGQSFLLVHLNRLIRKLDLNVIYIAGPGHGAPATLAH
                     CYLEGHYSEIYPDRSEDEAGMQRFFRQFSFPGGIGSHCTPETPGSIHEGGELGYSLSH
                     GYGAAFDNPDLIVAVMVGDGEAETGPLATSWHSNKFLNPIRDGAVLPILHLNGYKIAN
                     PTILARIPREELEALLTGYGHTPYFVEGDDPDTMHQHMAATLEQCVGEIRAIQQHARE
                     ANDATRPRWPMIVLRSPKGWTGPKEVDGHKVEGSWRAHQVPVLDPVTHSKSLKLVENW
                     LRSYEPESLFDEAGRLVEELRELAPEGTRRISANPHANGGMLCKTLDLPAFRDYAVTV
                     KKPAGSYTSPTEVLGTFLRDVMRRNMTNFRVFGPDETASNKLTAIYEASEKTWLADTV
                     ASDADGGELSVDGRVMEMLSEHTLEGWFEGYVLTGRHGLFATYEAFVHVIDSMFNQHA
                     KWLEKAKRDLGWRQPVPSINLLITSLVWRQDHNGFTHQDPGFLDVVTNKSPDVVRIYL
                     PPDANCLLSVADHCLRSRDYVNVIVADKQPHLQYLDMDSAVIHCTKGIGIWDWASTDQ
                     GVEPDVVMACAGDIATMEALAAVQILKERFPDLKIRFVNVVDLFRLMPENAHPHGLSD
                     RDFDSLFTTSKPVIFNFHSYASLVHKLTYNRTNHDNLHVHGYHEKGNINTPLELAIIN
                     QVDRFSLAIDVIDRVPALRGVGDHAKEWLRGQVIEHLAYAHAEGIDKEEIRNWTWKG"
     gene            complement(4612..4821)
                     /locus_tag="VHV99_20110"
     CDS             complement(4612..4821)
                     /locus_tag="VHV99_20110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637746.1"
                     /translation="MSPLPSQRRAPSPARQADSPGAVRDRRAELGRADEARAEQREQD
                     DARNDPNASKWRYRPPQRDDKEQQG"
     gene            complement(4853..4975)
                     /locus_tag="VHV99_20115"
     CDS             complement(4853..4975)
                     /locus_tag="VHV99_20115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637747.1"
                     /translation="MTSAIWGIVAGTALMFGASMYLRQLRLLHRPDRRWTGKKR"
     gene            5296..5421
                     /locus_tag="VHV99_20120"
     CDS             5296..5421
                     /locus_tag="VHV99_20120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637748.1"
                     /translation="MSTPSRLKAPLILALTLVLLCAGVAVLVGVFGLLVKLVGLA"
     gene            5517..6059
                     /locus_tag="VHV99_20125"
     CDS             5517..6059
                     /locus_tag="VHV99_20125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX3637749.1"
                     /translation="MTNHAEPSPPAQPEIDPDLLEVAQEVFDLARRGDAAMLAAVIEK
                     GVPPNLRNDKGDSLVMLASYHGHADAVRTLLERGADPDLRNDNGQTPIAGAAFKGFNA
                     VIETLLAHGADVEGPSPDGRTALMIAAMFNRTEIMDLLIAHGADPKARDANGVTAMDA
                     AGRMGAGDAQTRLKELGEKP"
     gene            complement(6056..6748)
                     /locus_tag="VHV99_20130"
     CDS             complement(6056..6748)
                     /locus_tag="VHV99_20130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX3637750.1"
                     /translation="MDTANAPPHDVVFLFDCDNTLLDNDHVLADLRAHMMREFGEQNC
                     VRYWEIFEDLRSELGYADYLGALQRYRQEHPRDTRLLLMSSFLIDYPFANRLYPGALD
                     AIRHVSKRGPAVILSDGDVVFQPRKIARSGLWEEVEGRVLIYIHKEQMLDQVMECYPA
                     RHYVMVDDKLRILSAMKKAWGEKLTTVFPRQGHYAFDPKEIASNPPADVTVERIGELA
                     ELGLDEVLSGKG"
     gene            complement(6764..7870)
                     /gene="gnd"
                     /locus_tag="VHV99_20135"
                     /pseudo
     CDS             complement(6764..7870)
                     /gene="gnd"
                     /locus_tag="VHV99_20135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923777.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
     gene            complement(8170..>8466)
                     /locus_tag="VHV99_20140"
     CDS             complement(8170..>8466)
                     /locus_tag="VHV99_20140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX3637751.1"
                     /translation="EGGILQTASWTLYEQTTFDTRRITSFDWSTYPIMRFASMPKGVE
                     VHLIDRPGLPFLGVGEAAQGPGAAVLANAIADATGARLRELPLAGARLKAALER"
BASE COUNT         1612 a         2782 c         2456 g         1616 t
ORIGIN      
        1 tcgggcatgc aagcgttctg tacgatccgc tcctaccttg ccaccttgcg taagcagaac
       61 cattcgctga ttgatgcact ggcctccagc tttgccggaa tcgtcgtttc gcctttgtct
      121 gccgctgaat agttacgcgg cggcaaagaa agtaagtgcc gccccgcaca ggggcaacac
      181 taatacacca ctaacaattc aaggaaaggc caaccccgta ggcatacaga caaaaagcgc
      241 cgcacgtgca aacagatcaa gcaaacccca acgccgcagg caaccatccg accaagcccc
      301 cgcacgcaca aaaaaacccc accccaaatc tgctactgtt cccaataccg cctcgcactg
      361 gaacccaaaa tgccaaccca cccctccact gcccacaatt cagcaagcgc acgcgcaagt
      421 tcagtaaccg cacgcctaac cgactcgcaa atcgccgccg aagcaaaaac ccgtctaacc
      481 tgggacgccg ccatccccaa aaacgcacta aaagtcaaag tctcccacgg ccgcatcaag
      541 ctactaggcg aacttaacca tgaacagcaa agaaccgcgg cactcgaaga cgtcacccgc
      601 ctgttcggcg taacagccgt cctcgaccac accacaataa aacaacccta acctcagccg
      661 gccccgcgca acatccctcg acaatgcctg gcaatctgct tctcctcctc cgtatgaatc
      721 gccctcacct tccccgccgg gtccccgtcc gataacccca aaaacgccag cccctcgcac
      781 actcgcttgc gtatctcctc actgtgctca ccgattcccc ccgtgaacac cagcaaatcg
      841 atcccgccca gcaaagccgc ataagcgcca atcgtcttgc gcacggcagt cgcaaatgca
      901 tccagcgcca gcgaagcatc cgcatcaccc gccgccgcgc gtttctccag cgcctgcata
      961 tcgctctcgc catcggcgaa accggccagg ccgctttgac gattcaacag cgtctccagc
     1021 gcatccgcat cgagcttctc cacgcgcatc agatacagca gcacgcccgg atcgaggtcg
     1081 ccgctgcgcg tgcccattgg cacaccgccg gtcggcgtca aacccatcga cgtatccacg
     1141 gatttgccat cctgcaaggc acacaaactc gatccgttgc ccagatgcgc gaacaccgct
     1201 cgccgcggca aagcggcgcc tagccgcgtc accagcgact catacgacag cccatgaaaa
     1261 ccgtagcgaa tcacgccggc ctccatgtaa cggcgcggca atgcaagctg cgccgcgcgc
     1321 ggtggcagcg tcgcatgaaa cgccgtatcg aagcaggcga aatgctgtgc atcggcaaag
     1381 atcgccgacg cctcgtcgat caacgccagc gccggcggaa tatgcaacgg cgcgaagtgc
     1441 acggcgtcct gcaaacgttg ccgcacttcc ggcgtcaagc gctgatgcgt tcgcagatgc
     1501 ggcccgccat gcaccacgcg atgccccacc gccctgggcc gctcgtggtt ctgcgcctcg
     1561 aggacccgcg cgagcttctg caacgcgtcg gtctgcgact ccagcagctg gtcctgctgc
     1621 accagtacgc ggccgtcggg cgacttgatc cgcaagctgc catcgcctcg tccgatgcct
     1681 tcggcgctgc cttccagcag caaggattcg tcatcgcccg agtgcgcgaa cagcccgaac
     1741 ttcagcgacg acgaaccgct gttcaacacg agaatcgtcg cttcgtcagt ctggcggtca
     1801 gtctgcttat gcgtctccat atcaagcctc gctcagcctt tccacgtcca gttgcggatc
     1861 tcttccttgt cgatgccctc ggcatgcgca tacgcaagat gttcgataac ctgcccacgc
     1921 agccactctt tggcgtgatc gccgacgccg cgcagcgccg gcacgcgatc gatcacgtcg
     1981 atagccagcg agaaccggtc gacctggttg atgatcgcca gctcgagcgg ggtattgatg
     2041 ttgcctttct cgtggtagcc gtgcacatgc aggttgtcgt gattggtgcg gttatacgtc
     2101 agcttgtgca ccagcgacgc atacgaatga aagttgaaaa tcaccggctt gctggtggtg
     2161 aacaacgaat cgaaatcacg atccgagagg ccgtgcggat gcgcattttc aggcatcagg
     2221 cggaacaggt cgaccacgtt gacaaagcgg atcttcaggt cgggaaagcg ctccttcaaa
     2281 atctgcacag ccgccagcgc ttccatcgtc gcaatgtcgc ccgcgcaggc catcaccacg
     2341 tccggctcaa cgccttgatc cgtcgacgcc caatcccaga tgccaatccc cttggtgcaa
     2401 tggataacgg ccgaatccat gtcgagatac tgcagatgcg gctgcttgtc ggcgacgatc
     2461 acattgacgt aatcgcgcga tctcaagcaa tggtcggcca cgctgagcag gcagttggcg
     2521 tccggcggca gataaatacg caccacgtcc gggctcttgt tggtgacgac atcgagaaag
     2581 ccggggtcct gatgcgtgaa gccgttgtga tcctggcgcc acaccagcga tgtaatcaac
     2641 aggttgatcg acggcaccgg ttgccgccag ccgagatcgc gtttcgcttt ttccagccac
     2701 ttggcgtgct ggttgaacat ggaatcgatc acgtggacga acgcttcata ggtcgcgaaa
     2761 agcccgtggc gtccagtcag cacgtaacct tcgaaccagc cttcgagcgt gtgctcgctc
     2821 agcatctcca tcacgcggcc gtccaccgag agttcgccgc cgtcggcatc gcttgccacg
     2881 gtgtcggcca gccaggtttt ctccgaggcc tcatagatgg ccgtcagctt gttgctggcg
     2941 gtttcatcgg ggccgaagac gcggaagttc gtcatgttcc tgcgcatcac gtcgcgcaga
     3001 aacgtgccga gcacctcggt gggcgacgtg tacgagccag caggcttttt caccgtcacc
     3061 gcgtagtcgc gaaacgccgg caggtcgagc gttttgcaca gcatgccgcc attcgcatgc
     3121 gggttggcgc tgatgcggcg cgtgccttcg ggcgccagct cgcgcagttc ttcgaccaga
     3181 cgcccggctt cgtcgaatag cgactccggt tcataactgc gcagccagtt ctcgaccagc
     3241 ttgagactct tgctatgggt gaccggatcg agcaccggca cctggtgcgc acgccacgaa
     3301 ccttccacct tatgaccgtc gacttccttc ggcccggtcc agcctttcgg cgagcgcagc
     3361 acgatcatcg gccagcgcgg ccgtgtggcg tcattggcct cgcgcgcatg ctgctggatc
     3421 gcgcggattt cgccgacgca ttgttcgagc gtcgcggcca tgtgttgatg catggtgtcc
     3481 ggatcgtcgc cctcgacgaa atacggcgtg tggccgtagc cggtcagcaa ggcctcgagt
     3541 tcctcgcggg gaatccgcgc gaggatcgtc ggattggcga tcttgtagcc gttcagatgc
     3601 aggatcggca acaccgcgcc gtcgcgaatc gggttgagga acttgttcga gtgccaggag
     3661 gtagcgagcg ggccggtttc cgcctcgccg tcgccgacca tcaccgcgac gatcagatcg
     3721 gggttgtcga atgcggcgcc gtaaccatgc gacaggctat agccgagttc gccgccttcg
     3781 tgaatcgagc ccggagtttc gggtgtgcaa tgcgagccga tcccgccggg aaacgaaaac
     3841 tgccggaaga agcgttgcat gccggcttcg tcctcgctgc ggtcgggata gatctccgag
     3901 taatggcctt cgaggtagca atgggccagc gtggcaggcg cgccatggcc cggacctgcg
     3961 atatagatca cgttcagatc gagtttcctg atcagccggt tcagatgcac cagcaggaag
     4021 ctctgtccag gatccgagcc ccaatggccg agcaagcggt tcttgatatg ttcgggtttg
     4081 agcggttcgc gcaacagcgg attgtcgcgc aggtagatca tgccggccga gagatagttg
     4141 caggcgcgcc agtagcgatc catcttgcgc aacgtgtcgg gctccagggt ttgcggcggg
     4201 gtcggacggg agggtgcttc ggccatggcg tcactccttg gaaaggactg tcaggcaggt
     4261 tagcgggtgc tgcagcggtt gttcctgacg cggccggcgc agaaaaacca gccatgccgg
     4321 ccgtcctgtc atacgcctga catcaggaaa gcgtagcgcc aaaggcccgt gacgcaaggc
     4381 ttgggagatc ggccggggat cgtcaggggg tgtgacagga agcgcgcgtc atggttcgac
     4441 gcgatcgtcc acagcttgat cttgcgacgc gcgtgcatcg ctttcgtgtg cgcgtctcgt
     4501 gtgtgcggca ggcgcagata gggtagcggc cggtccgatt gccggcccga gcgatggcta
     4561 gtgcccgctc gactcttcgt acgttcgtac gctggtgttc agtcgcgggc tttaaccctg
     4621 ctgctcctta tcgtcgcgtt gcggcggccg gtagcgccat ttgctggcat tgggatcgtt
     4681 gcgcgcgtcg tcttgctcgc gttgctcggc gcgcgcttca tcggcgcgac cgagttcggc
     4741 gcgccggtcg cgcacggcgc ccggcgaatc ggcttgacgt gccggcgacg gcgcgcgtcg
     4801 ctgagaaggt agtggtgaca tggagcttcc ttttttgagg aggtgggacg aatcaacgtt
     4861 tcttgcccgt ccagcggcgg tcggggcggt gcagaagacg aagctgacgc aggtacatcg
     4921 aagcgccgaa catcagcgcc gtgccggcca cgatgcccca gattgcggat gtcatgattt
     4981 ttcctttagc ccgcggcccg cacagcattt gatcacctgc aacgtgacgg tgggcaaccg
     5041 attgcgcgcg cggcggatgt tgcacggacc tgctgcacag atctgatcat gccgcgcgcc
     5101 aagcaaggat catgcgctca ttcggcgggt gtgtctgtac gctgttggac agtcggtgcg
     5161 ctggcggccg ccacgcttgc gctagacttt aagcgagcat taagtaaagg cgattaagct
     5221 cctgccgcca agtgcgcgac ggcggatcgc atccggcgct cctgtactgc tcactcatta
     5281 ttctgaacca ccaccatgtc gacacccagc cgcctcaaag cccccctgat tctcgctctc
     5341 acgctcgtgc tgttgtgcgc cggcgtcgcc gtactcgtgg gcgtgttcgg cctgctggtc
     5401 aagctggtgg gccttgccta gcatgctgcc gggcgggcgg gcgcggcgcg gaatgtgtat
     5461 cctgatggat cttcccggtg ccgtcccggc ggccagaatc attgcaagga gattccatga
     5521 cgaaccacgc tgaaccttcc ccgcccgccc aaccggagat agatccagat ctgctcgaag
     5581 tggcacaaga agttttcgac ctcgcacggc gcggcgatgc cgcgatgctc gccgcggtga
     5641 tcgaaaaggg cgtgccgccg aatctgcgca acgacaaggg cgacagtctc gtgatgctgg
     5701 cgagctatca cggccatgcg gatgcggtgc gcaccctgct cgaacgcggg gccgatccgg
     5761 atctgcgcaa cgataatggc cagaccccca ttgcgggcgc cgcgttcaag ggtttcaacg
     5821 ctgtcattga aaccttgctc gcgcatggcg ccgacgttga aggcccctcg ccggatggcc
     5881 gcaccgcact gatgatcgcc gcgatgttca atcgcaccga gatcatggac ttgctgattg
     5941 cccacggcgc ggatcccaag gctcgcgatg ccaacggcgt cacggcaatg gacgccgccg
     6001 gcaggatggg agccggggat gcgcaaacgc gtttgaagga actgggagaa aagccctaac
     6061 ccttcccgct caaaacctca tccagaccca attcggccaa ctccccaatc cgctcgactg
     6121 tcacatccgc gggcggatta ctcgcaatct ccttgggatc gaatgcatag tgcccctggc
     6181 gaggaaagac ggtagtcaac ttctcgcccc aggccttctt catggcgctc agaatccgca
     6241 atttgtcatc caccatgacg tagtgcctgg ccggataaca ctccatcacc tgatcgagca
     6301 tctgctcttt atggatgtag atcaaaaccc gcccttcgac ctcctcccat aagccggagc
     6361 gggcaatctt tcgcggctgg aacacgacgt ccccgtcgga caggatcact gcgggcccgc
     6421 gcttcgacac atgccggatc gcatccaatg cccccggata cagacggttc gcaaacggat
     6481 aatcgatcag aaacgacgac atcaacagca ggcgcgtgtc gcgcgggtgc tcctgccgat
     6541 accgttgcaa cgcgcccagg taatcggcgt agccaagctc cgagcgcaag tcttcgaaaa
     6601 tttcccaata ccgtacgcag ttctgctcgc cgaattcccg catcatgtgc gcgcgcaggt
     6661 ccgccagcac atgatcgttg tccagcaacg tgttatcgca atcgaacaga aacacgacgt
     6721 cgtgaggggg ggcgttagcg gtatccatgg cggctccggt gaattagcgc ttcgttccgc
     6781 cgtcgaccgg cgtggccggt ttctcgacgt gaccgccgaa gtcattgcgc atggccgaca
     6841 acacccggtt cgcgaaatcc gcctcgccgc gcgagctgaa acgcgcgaat agcgcggcgc
     6901 tcagcacggg cgtcggcaca ccttcgtcga tcgccgcggc caccgtccag cgcccttcgc
     6961 ctgaatccga tacgcgtcct gtgtagtgcg tcagatcggc gtcgctgacg agcgatcctg
     7021 ctatcaggtc cagcaaccac gaaccgatca cgctgccgcg ccgccatacc tcggtaacct
     7081 cggcgagatt caggtcgtat tggtagagct cgggacggcg cagcggcgaa gtttcagcgt
     7141 cggcttcgcg cgcctgtttg ccggcatcgg cgtggcgcaa aatgttcagg ccctcggcat
     7201 acgcggccat catgccgtac tcgatgccgt tatggaccat cttcacaaag tgccccgcgc
     7261 cctggggacc gcaatgcagg aaaccctgat cggccgtact ctcgccggcg ctacggcccg
     7321 gtgtgaccgg tgcggagccc gcgcccggcg cgagtgtcga gaaaatcggt tcgagccgct
     7381 tcacgacctc ggcctcgcca ccgatcatca gacaatagcc gcgctcacgt cccgcgacgc
     7441 cgccgctcgt gccgacgtcc acatagtgca gctgacgcct ggccagatcg gcgccgcggc
     7501 gaatgtcgtc gtggtaatag gagttgccgc cgtcgatgac gatgtcgccc ggttccagca
     7561 agggcacgag gttctcgagc gtcttgtcga cgacggccgc cggcaccatc agccacactg
     7621 ctcgcggttt ctccagctgc gcgatcaggt ctgcgagcga tgttgcgccc gcgaccccat
     7681 cctgtttcag cttttccacc gcggccggtt gcacgtcgta agcgatacat tgctgaccgc
     7741 ccttcgtcag gcgtcgcacc atgtcggcgc ccatgcgccc caatccaatc atgcctagct
     7801 gcatatctcg ctccggtaag tagctcgttg cgctgctcga tctgtttcgc tgtgtttcgc
     7861 tgtgtttcgc tgtgtttcgc tgtgtttccg tgtttccgta tgttgcgcca cgccgtgtgc
     7921 ttcagccgcg gcaagtggct ctcctaactg tagttgccct cgcgtgatcc gacggacaca
     7981 cggtagcagc ctcgcgtcat cggtgcagcg aatcgggcat gtccggctgc accaaacgac
     8041 gatacgcctc tttgcagaga ttttgcgccg cctgaagttc caatcgtgcg tcgatgcgag
     8101 ccgtagtcgg gacgaatgtt tctatttttc tcacttaatt cttacatttc ccatgttgca
     8161 cgcgggcgct tacctttcca acgcagcttt caaacgcgca ccggcaagcg gcagttcgcg
     8221 caggcgcgcg ccagtcgcat cggcgatcgc attcgccagc acggccgcgc ccggcccctg
     8281 cgccgcttca ccgacaccca gaaacggtaa gccgggccgg tcgatcagat ggacttcgac
     8341 ccccttcggc atcgacgcaa accgcatgat cggatacgtg ctccagtcga aactggtgat
     8401 gcggcgcgtg tcgaaggtgg tttgctcgta gagcgtccag ctcgccgttt gcaggatgcc
     8461 gccctc
//
LOCUS       DATASH010000754         9523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1707353,
            whole genome shotgun sequence.
ACCESSION   DATASH010000754 DATASH010000000
VERSION     DATASH010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 9523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9523
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1707353"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..840)
                     /locus_tag="VHV99_20145"
     CDS             complement(181..840)
                     /locus_tag="VHV99_20145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013341153.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HEX3637752.1"
                     /translation="MADHKSTGRPSRSAQSDPDVEPDQQPATLDACRRCALWRDATQP
                     VPGDGAAHAAIMLVGEQPGDAEDVQGHPFVGPAGALLDKALEEAGVVRNKVYVTNAVK
                     HFKWTPRGKRRMHKTPGQREIDACAYWLEREFRMLAPRVVVALGATALKAVLEDSHVR
                     LQDALGKTIEHGGHRVVATYHPSFALRAPDQATRERVYAEIVAALRTANRLSRPRGRS
                     G"
     gene            1248..2465
                     /locus_tag="VHV99_20150"
     CDS             1248..2465
                     /locus_tag="VHV99_20150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012404502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEX3637753.1"
                     /translation="MISTAADNDPSGIATYSLAGAQFGYDLLWTCVLSYPLIVALQLV
                     SARIARLTGKGLTTNMREHYSRIWFFLAVARFLIANTFNIAADVIAMGVAAQLLWHGS
                     MPGFAAVFLVLSVALQWFVPYPKYARVLRWLALSLFAYVVVDITLRLPWSTIAIKALI
                     PHVTWSVHYLEMLLAVLGTTISPYLLYSQAEQQVEAQRPEQRTHADRSANGSRHDGFR
                     SLRVGTLIRTAMSNAVALSIMIAAAASLHAGGEAVDTPASLAGALAPIAGQFASHLLG
                     LALLSSALLALPPLAGSAAHAVASGLNWHNNASNDRRIAYTHIAVVALGGALGVALAA
                     MRFEPLRILYWSAVLNGTTATPVMVLLVLLSTKHSSVGDLSSHWTLRVLAWLATVSMG
                     AAVAARYACGWLS"
     gene            complement(2569..4104)
                     /locus_tag="VHV99_20155"
     CDS             complement(2569..4104)
                     /locus_tag="VHV99_20155"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HEX3637754.1"
                     /translation="MTTGNRKSGLTSLGPISWGTHLCHFYETRNELLSVTANFLQAGL
                     AQREAGVWVAPALLGPDKARHMLTRAVPALAEYIDSGQLRILDGAQLYAPSRTGFNGS
                     ATVGLWSAAINDALGRGFEGLRVAGDAVCNEHTDWAALLDYEATLKHRLAGSPIVGLC
                     TYCVQGLRAAQTRQIERLHDATVAPSSTECVDRAAPKVGRRAIPVEPQQLGGVSQQVG
                     GEPQQAAALAETSIEAAKQFAVGPMIGHEIRNAITPLGLLARLLNTQYGHDEQLTRYA
                     LVLSRQANLLGQLAQQLMAPAHAQCASSLESEGEVVVNAQPAQPAQPAHVNVDDLLAH
                     CIQVAEAGVPGHLPINLAKSAAPLLVRGDFCKLTQVFVNVMVNALKYSSPTDAVSVMA
                     ARAGRFCYISVRDNGIGIAPRELKTIFNPYTRGLEGRALGVNGLGLGLSVVREIVASC
                     GGTVEAFSEGPGTGSEFVVRLPLANLPINAPVNEDAFEANVPGEVCAGLADMGAGQVL
                     TGL"
     gene            complement(4358..4764)
                     /locus_tag="VHV99_20160"
                     /pseudo
     CDS             complement(4358..4764)
                     /locus_tag="VHV99_20160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068333.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
     gene            5012..6856
                     /locus_tag="VHV99_20165"
     CDS             5012..6856
                     /locus_tag="VHV99_20165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HEX3637755.1"
                     /translation="MSATVGDFIIARLHAWGVRRIYGYPGDGINGLFGALNRAQTAAR
                     KHQKDKDQDHPTRPIEFVQVRHEEMAAFMAAAHAKFTGELGVCIATSGPGASHLITGL
                     YDARMDHMPVLAIAGQQARAALGGHYQQELDLVSLFKDVAGAFVQQAVVPAQVRHLID
                     RAIRTALAERKVTAIVLPNDLQDLPYEAPGRKHGTLHSGVGYRAPRLVPYPDDLQQAA
                     DVLNAGKKVAILVGAGALHATDEVIAVAGKLGAGVAKALLGKAVLADDLPWVTGSIGL
                     LGTKPSYDLMSECDTLLMIGSGFPYAEFLPKEGAARGVQIDIKADMLSLRYPMEVNLV
                     GDSVETLRALLPLLEEKSDRAWRRKIEGWTEDWWKTLDKRAHEAGKDAVNPQRTVWEL
                     SRRIPANSIVTSDSGSVANWYARDLKVQRGMMCSLSGGLASMGAAVPYAIAAKFAYPE
                     RPVISLVGDGAMQMNNMAELITVSKYWQGWTDPRWICMVLNNADLNQVTWEQRAMEGD
                     PKFEASQEIPSVPYHKFAEMIGLKGIYVGDAEQMGAAWDEAMAADRPVVIEVKADPNI
                     APLPPHITLAQAKAFASTLLKGDPDQGNVIVETARQVLGAVLPGHHDK"
     gene            6901..7608
                     /locus_tag="VHV99_20170"
     CDS             6901..7608
                     /locus_tag="VHV99_20170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012732928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEX3637756.1"
                     /translation="MNAMALTVSWQDVAVRLAVALAAGVVIGLNRGESGKAAGLRTTL
                     LVCLAACIAMLQVNVLLSQAGKPEGSFVQLDLMRLPLGILSGVGFIGAGAILRKDGLI
                     TGVTTAATLWFVTVIGLCAGGGQIALAITGLVLGIVVLLVLGAIEQRLPRNRRGRLTL
                     EYDLAADTRARLIERLEQHRCDVRSRALRLVTDTARLEERFEVHWRAPLGNDAVERLM
                     ADLIGKEAQAITWSTDG"
     gene            complement(7650..8423)
                     /locus_tag="VHV99_20175"
     CDS             complement(7650..8423)
                     /locus_tag="VHV99_20175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX3637757.1"
                     /translation="MTRQWQFTASRLLIAATFGVLISGCTSMPWESTPFYSSAPSHPT
                     TLGTVPVPAGYYRANQGDTLAGIASAYGRKPQEIAAWNGLAANSAVNPGQVLRVAPPM
                     ASGSVVTPPVNVAGQNGSVTTPGATGAPGVPQPGVLLWPLRGPVLKAFAPGKSNGIVI
                     GGHPGDPVKAAATGRVVYAGTGIEAYGPLIIIKHDDSLITAYGQNSTLLVKEGDAVAQ
                     GQTIGEVGVDSRGVASIQFEVRQNGQPVDPLTWLPKSGG"
     gene            complement(8584..8895)
                     /locus_tag="VHV99_20180"
     CDS             complement(8584..8895)
                     /locus_tag="VHV99_20180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004190911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX3637758.1"
                     /translation="MKIIRSKSFTATRPWGALDIANMGGITTRLHWTDQPYKWHVNDG
                     EEVFAVLDGRVEMHYREDGVEQSAILETGDVFYASVGTEHVAHPIGVARILVVEAAGS
                     V"
     gene            9143..9463
                     /locus_tag="VHV99_20185"
     CDS             9143..9463
                     /locus_tag="VHV99_20185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006760526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637759.1"
                     /translation="MNKATFLAVQLNVDDVAASPGLVELLNTHLVFAADVAEAADALV
                     QLASIAGLSSGVEASVEIPAVAIERLREALDNLSAYDESWLLALEPSRALFDEIGRRH
                     RTLH"
BASE COUNT         1799 a         3060 c         2874 g         1790 t
ORIGIN      
        1 cagtcgctcg atagctggaa gcactggtac gacgaggcac cacagtcatc gaattcggcg
       61 gcatagacca tacgctctcg ccaaataatc gctgaccgac tagaacggcc ctgaagctgc
      121 aaactttgac tccctcctaa accagtagtc gcaggtccga atccactacg gcccgtcata
      181 tcagccggac cgtccgcgag gccggctcaa ccggtttgcg gtgcggagtg ccgccacgat
      241 ttcggcgtag acacgttcgc gggtcgcttg atccggcgca cgcaacgcga atgacgggtg
      301 ataggttgcc acgacccgat gcccgccgtg ttcgatcgtc ttgccgaggg cgtcctgtaa
      361 gcggacgtgc gaatcttcca gcaccgcttt cagcgcggtt gcgcctaatg cgacgacgac
      421 ccgcggcgcc aacatgcgga actcccgttc caaccagtag gcgcatgcat cgatctcgcg
      481 ttggcccggc gtcttgtgca tgcggcgctt gccacgcggc gtccatttga agtgtttgac
      541 ggcgtttgtg acgtagacct tgttacgcac cacgccggcc tcctcgagcg ctttatcgag
      601 caacgccccg gctggtccga cgaacggatg gccttgcaca tcttccgcat cgcccggctg
      661 ctcgccgacc agcatgatcg ccgcgtgcgc ggcgccgtcg cccggcactg gctgtgtggc
      721 gtcgcgccat aacgcgcaac ggcggcaggc gtccagggtt gccggctgct ggtcgggttc
      781 cacgtccggg tcggattgag cactgcggct cggacgcccg gtagacttgt ggtctgccat
      841 ggtcagcatt cctttccata gtgccgctac cggcgcggtc cgagcaacgg aagggtcgtg
      901 aaaaaaggcc cctacccatc gcgagacgac cgggcaatca agcgcggggc tcgacgacag
      961 gatctcgaaa tccagcaatt gccgtgccct tcatgccctg ccacaggtac ttatcttgct
     1021 gctgcactaa cgacgcacac gacgcacgcg cgactcccaa gccctgttcg tgcggccacg
     1081 agtgacgttt gcgtaacgac ttcaaccggt gcgaaaaatc gatcgaacgc cgacatgagc
     1141 ggcatgtcgc ccacacagcg atttcaggag cgaacattac ctaccatcgc gacagtgttc
     1201 gaggtgagcc cgccgcccac tcctgggcgg ccgatattgg gccgggcgtg atctccaccg
     1261 cagccgacaa cgacccaagc ggtatcgcca cctattcgct tgcaggggcg cagttcggat
     1321 acgatctgct gtggacgtgt gtgctgtcct atccgttgat agtcgccttg cagctcgtat
     1381 cagcgcgtat cgcgcgtcta acgggcaagg gattgacgac caacatgcga gagcattact
     1441 cgcgcatctg gttttttctc gcggttgcac gcttcctgat cgccaacacc ttcaatattg
     1501 ccgccgatgt catcgcgatg ggtgtagccg cccaattgct gtggcacggc tcgatgcctg
     1561 gcttcgcagc ggtgttcctg gtgctctccg tcgcgctgca gtggtttgtt ccgtatccca
     1621 agtacgcgcg cgtgctgcga tggctggcgc tgtcgctctt cgcttacgtc gtcgtcgaca
     1681 tcaccctccg tcttccctgg tcgacgatcg cgatcaaagc actgataccc cacgtgacct
     1741 ggtccgtgca ttacctggag atgctgctcg cggtactcgg cacgaccatc agcccgtacc
     1801 tgctctattc acaggccgaa cagcaggtag aggcgcagcg ccctgaacag cggacacatg
     1861 ccgatcgctc agccaatgga tcgcgtcacg atgggttccg aagcttgcgc gtcggcacac
     1921 tgattcgaac ggcaatgtcc aatgccgttg cgttatccat catgatcgcc gcggcagcaa
     1981 gcctccatgc aggaggcgag gccgtcgaca cgcccgcttc cctcgcgggc gcgcttgcgc
     2041 cgatcgcggg ccagttcgct tcccatctgc tggggctggc attgctcagc agcgcgttgc
     2101 ttgcgttacc gccgcttgcc ggttccgcgg cgcacgcagt cgcgagtggg ttgaactggc
     2161 ataacaacgc ctcgaatgac cggcgcatcg cctatacgca catcgcggtc gtcgcactag
     2221 gcggcgccct cggcgtcgcg ctggcggcaa tgcgattcga gccgctgcgc attttgtact
     2281 ggagcgcggt actcaatggg acgaccgcaa cgcccgtgat ggtgctgctg gtgctgctca
     2341 gcacgaagca ctcgtcagtg ggcgatctga gttcgcactg gacgttgcgc gtgctggcct
     2401 ggctggcaac cgtcagcatg ggcgccgccg ttgccgcacg ctatgcgtgt ggatggctca
     2461 gttgaacttg atgctttggc ggcgcacgac gagaaacggc atgaaggaaa gctgcgtaaa
     2521 ggaaaacggc ttaaaagctc atgcagcgtc tttccctgtc ccaacattct aaagacccgt
     2581 caacacttgc ccggcaccca tgtcggccag tccggcgcaa acctcgccgg ggacatttgc
     2641 ctcgaacgca tcctcattca ccggtgcgtt gatgggcagg ttcgcaagcg gcagacgcac
     2701 cacgaattcc gacccggtcc cgggaccttc ggagaaggct tcgaccgtac ccccgcagct
     2761 ggcaacgatt tcacgaacca ccgacaagcc aagcccaaga ccgttgacac ccagcgcccg
     2821 accttcgaga ccgcgcgtat agggattgaa gatcgttttc agttcgcgcg gcgcgattcc
     2881 gatgccgttg tcgcgcacgc tgatatagca gaagcgcccc gcacgcgccg ccatcaccga
     2941 gactgcatcg gttggggagg aatatttgag cgcgttcacc atgacgttga cgaacacctg
     3001 cgtcagcttg cagaagtcgc cccgcacgag gagtggcgca gcacttttcg ccagattgat
     3061 tggcaaatgc ccggggacac ccgcttccgc gacctgaatg caatgcgcga gcaggtcatc
     3121 gacattgacg tgcgccggtt gcgccggttg cgccggttgc gcgttcacca cgacctcgcc
     3181 ctccgattcc agcgagctcg cgcactgagc atgggccggc gccattaatt gctgagccaa
     3241 ctgccccagc aggttggctt gccgtgacag caccagggca taccgagtga gctgttcgtc
     3301 gtggccatat tgtgtattca gcaaacgtgc aagcagaccg agcggcgtaa tcgcattgcg
     3361 aatctcatgg ccgatcatgg gcccgacggc aaactgcttt gcggcctcaa tggaggtctc
     3421 ggcgagcgct gcggcctgct gcggttcacc accgacctgc tgcgatacac cgccgagctg
     3481 ctgcggttca actggtatcg ctcgccgtcc gactttcggt gcggcgcggt ccacgcattc
     3541 agtcgatgac ggcgcaaccg tcgcatcgtg cagacgttcg atctgtcggg tttgagcggc
     3601 gcgcagcccc tgtacgcagt aagtgcacaa gccgacgatc ggactcccgg cgagcctgtg
     3661 cttcagcgtc gcttcatagt cgagcaatgc tgcccaatcg gtatgctcgt tgcagacagc
     3721 gtcaccggca acccgtagac cttcgaagcc gcgtccgagc gcatcgttga tggcagccga
     3781 ccataagccc accgtggcac tgccgttaaa gccagtgcga gacggcgcat agagttgcgc
     3841 gccatcgaga atccgcaact gaccggagtc gatatattcg gcgagtgcag gcactgcccg
     3901 tgtgagcata tgacgggctt tgtccggacc cagcaacgcg ggtgcgaccc atacgccggc
     3961 ttcccgctga gcgagtccgg cctggagaaa attcgccgtc acgctgagta attcgttgcg
     4021 cgtctcgtag aaatgacaaa ggtgggtgcc ccaggaaatg ggcccaagtg aagtaagtcc
     4081 gcttttacga tttccggtgg tcatcgcttc cctaaaaata cgtgggccaa gaaccacagc
     4141 agcgctgttc gacagaatcg acagcgcgtt cgaaaaaata aaaaatgtca ccgcaccggg
     4201 cgcgcatgtc cggcagtcct gactgaaccc aggaagcaga aagtcacgcc cgattggtta
     4261 tacgagaatc acaaggaaaa caaagtacgt gacaggcatt tttgaatgaa tcctcgtttt
     4321 ccctagccaa tccttttgcc tggcagcgat ccattcgtca tacgcatgtc gcaatgtgcg
     4381 tgttcacatt aagccgtggc gtatggtcgt caaagggcgg cttcggcacg aaaccgcgca
     4441 tcgcgcgggg ttcaactatc ggccagtatg tcgagccggc atactggaaa taacgccgtt
     4501 cattgccctt cctgacgcgc cgacccgaaa gggcgtcaat cgcgcacgac gagacatccg
     4561 ccatgccgac gaattgaaac gcgacctggc caatggccga tcgttgcagt cgatatcgat
     4621 cacggcgcgc gctgcgactg ccgtgatatg cccgtgcgaa tcgttcagcc cgccctggac
     4681 gatacaggca gccacggcgc gcgcgacatt agccacctct tcctgcatgt ctctgtcgca
     4741 atcgagcgta gcgtgaccgg tcgcgtcaac gcggtctgaa agaggggaac aaattttgcg
     4801 gccggaacgt tccgagcgtg ctcgcgccat cgcacggcat tgaccgttcc gagcctgctc
     4861 gccccatcgc acggcattga cgattacagc ttgcaatatc gcggaacggg acttgcaaca
     4921 cacgtcggat gtgtacaaca ggcggcgata tggcgaggcc aaatcgtagc tgccgctgcc
     4981 gtgccgcttt aatcgcattc aaggagacag catgtccgcg accgttggcg acttcatcat
     5041 cgcgcggctt cacgcctggg gcgtgcgccg catttacggt taccccggcg acggtatcaa
     5101 tggcctcttc ggtgccctca accgtgccca gaccgcggca aggaaacacc agaaagacaa
     5161 agaccaggac caccccaccc gtcccattga attcgtccag gtccgccacg aggaaatggc
     5221 cgccttcatg gccgccgccc atgccaaatt caccggcgaa ctcggggttt gcatcgccac
     5281 ctcgggcccc ggcgcgtcgc atctgatcac cggcctttac gacgcacgaa tggatcacat
     5341 gccggttctc gccatcgcgg ggcagcaagc ccgcgccgcg ctgggtggcc actatcagca
     5401 ggaactcgac ctggtctccc tgttcaagga cgtcgccggt gccttcgtcc agcaggccgt
     5461 tgtgccggct caagtccgcc atctgatcga ccgtgccatc cgcaccgcgc tcgccgaacg
     5521 caaggtcacc gcgatcgtgc tgcccaacga tctgcaggac ctgccctatg aagcacccgg
     5581 ccgcaaacac ggcacgttgc actcgggcgt cggctatcgc gcgccgcgtc tcgtgccgta
     5641 tcccgacgat ctgcagcaag ccgccgacgt cctcaacgcc ggcaagaaag tcgcgatcct
     5701 cgtcggtgcg ggcgcattgc atgccactga cgaagtgatc gccgtcgccg ggaaactcgg
     5761 cgcgggcgtt gccaaagcac tgctcggcaa agctgtcctg gcggacgatt tgccttgggt
     5821 caccggctcg atcggcctgc tcggcaccaa acccagttac gacctgatga gcgagtgcga
     5881 cacgctgctg atgatcggct cgggctttcc ttatgcggaa tttttgccga aggaaggcgc
     5941 ggcgcgcggc gtgcagatcg acatcaaggc cgacatgctg agcctgcgtt atccgatgga
     6001 agtgaatctg gtcggcgata gcgtcgaaac cttgcgtgca ttgctgccgc tccttgaaga
     6061 aaaaagcgac cgcgcctggc gcaggaagat cgaaggctgg accgaggact ggtggaagac
     6121 gctcgacaag cgcgcgcacg aagccggcaa ggatgcggtc aacccacagc gcacggtatg
     6181 ggaattgtcc aggcgcattc ccgccaactc gattgtgacg agcgactccg gctcggtcgc
     6241 gaactggtac gcgcgcgatc tgaaagtgca acgcggcatg atgtgctcgc tctccggcgg
     6301 cctcgcgtcg atgggcgcgg ccgtgcctta tgcgatcgcg gcgaaattcg cctaccccga
     6361 acggcccgtg atctcgctgg tcggcgacgg cgcgatgcag atgaacaaca tggccgaact
     6421 gatcacggtc tcgaagtact ggcaaggctg gaccgatccg cgctggatct gcatggtgct
     6481 gaacaacgcg gacctcaacc aggtgacatg ggaacagcgc gcgatggaag gcgatccgaa
     6541 gttcgaggcc tcgcaggaga ttccgtcggt gccgtatcac aagttcgcgg aaatgatcgg
     6601 cctgaaaggc atttacgtcg gcgacgccga acagatgggc gccgcctggg acgaagccat
     6661 ggcggcggat cgaccggtgg tcatcgaagt caaggccgat ccgaatatcg cgcccctgcc
     6721 gccgcatatc acgctcgcgc aggccaaggc ctttgcgtcg acgctgctga aaggcgatcc
     6781 ggatcaaggc aatgtcatcg tcgaaacggc caggcaggtg ctcggcgcgg tcctgcccgg
     6841 ccatcacgat aaatagagcg cgcgtgacgg cgcctcagcc gacctcattg ggggacgggt
     6901 atgaacgcga tggcattgac agtcagttgg caggacgtag ccgttcgcct tgcggtcgcg
     6961 ttggcagccg gcgtggtgat cggactgaat cgcggcgaat ccggcaaggc ggcgggtcta
     7021 cgcacgacac tgctggtttg ccttgctgcc tgcatcgcga tgcttcaggt caacgtgctg
     7081 ttgtcgcagg ccggcaaacc cgaaggctcg ttcgtgcagc tcgatttgat gcgcctgccg
     7141 ctcggcattc tgtcgggggt cgggttcatc ggcgccggtg cgattctgcg caaggacggt
     7201 ctcatcacgg gcgtcacgac cgctgcaaca ttatggtttg tgaccgtgat cggtttgtgc
     7261 gccggcggcg gccagatcgc cctggctatc accggcctcg tgttaggcat tgttgtgctg
     7321 ctagtactcg gtgcgatcga gcaacgtttg cctcgcaaca ggcgtggacg gctgacgctc
     7381 gaatacgacc tggcggccga tacccgcgcc agattgatcg agcgcctcga gcagcaccgg
     7441 tgcgacgtca ggtcgcgcgc gctgcgactc gttaccgaca cggctcggct cgaagaacgg
     7501 tttgaagtac attggcgcgc tccgctcggc aacgatgcgg tggagcgcct catggccgat
     7561 ctcatcggca aggaggctca ggcaataacc tggtcaaccg atggctgact gttcgaatcg
     7621 aacgtacctc aagccgggat catctggaat cacccacccg acttcggcag ccacgtcagt
     7681 ggatcgaccg gttggccgtt ctggcgcact tcgaactgga tcgacgccac accccggcta
     7741 tccacaccga cttcgccgat ggtttggcct tgcgcgacag cgtcgccttc cttgaccagc
     7801 agcgtgctgt tctgcccgta ggcggtaatc agcgagtcgt cgtgcttgat gatgatgagc
     7861 ggaccgtacg cctcgatacc cgtgcccgca taaaccacgc ggccggtggc ggcggccttg
     7921 accggatcgc ccggatggcc gccgatcacg ataccgttcg acttgccggg cgcgaaggcc
     7981 ttcagcacgg gaccccgcaa cggccacaac agcacacctg gctgcggaac gccgggggca
     8041 ccggtcgcgc caggcgtcgt gaccgaacca ttctgaccgg caacattaac cggcggtgtc
     8101 accacgctac ccgacgccat gggcggcgcc acccgcagca cctgccccgg attgacggcg
     8161 gaattcgccg cgaggccgtt ccacgccgca atctcttgcg gcttgcggcc atacgccgac
     8221 gcgatgcccg ccagcgtgtc accctgattg gcgcggtagt aaccggccgg cacagggacc
     8281 gtgccgaggg tagtgggatg ggacggcgcg ctgctgtaaa acggcgtgct ttcccacggc
     8341 atcgacgtac agccgctgat caacacgccg aaggtggccg cgatcagcaa ccggctcgcc
     8401 gtgaattgcc attgtcttgt catgtgattt tcctcgacac tttgattcga tcggtttcaa
     8461 tcagccccaa gcatttgccg tgccgcccgg ctcggctcgg ctcggctcgg ctcggctcgg
     8521 ctcggcccgg ctcgtatcca ttatgccgcc gtgcgagtcg gcaccgcatg ggctgctgag
     8581 ccctcagaca ctgcccgcgg cctcgaccac cagaatgcgc gccacgccga ttggatgcgc
     8641 gacatgttcg gtacccaccg acgcgtagaa cacatcgccc gtttccagaa tggccgactg
     8701 ttcgacgccg tcttcgcggt agtgcatctc cacacggccg tcgagcacgg cgaacacttc
     8761 ctcgccgtcg ttgacatgcc atttatacgg ctgatcggtc caatgcaggc gcgtcgttat
     8821 gccgcccatg ttggcgatat cgagcgcgcc ccatggacgc gtcgcggtga agctcttgct
     8881 gcggatgatc ttcatggtga atagcggttg agaagccgac gctgtccgcg tgttatccgt
     8941 gccgcatacc gaagcgcccg cctcggcgag atcacgcgtt ccgactggcc ccattatagg
     9001 tgttgcagcg gtcacgaccg caggcacgag gccgctcggg cgcattcgtg tgatcattga
     9061 atcctttcgg ccctcgccta tactcagtca aactaacgcg acgcaccgta cgcaaacgcc
     9121 tgaatcctgc gaggagttgg ggatgaacaa agccaccttt ctggccgtgc aactcaatgt
     9181 cgacgatgtt gcggcgtcgc ccggtttggt cgagttgctg aacacccatc tggttttcgc
     9241 ggcagacgtc gccgaagccg ccgacgcgct ggtccagctg gcgagcatcg cgggtctgtc
     9301 gagcggcgtc gaagccagcg tcgagatacc cgccgttgca atcgaacggt tgcgcgaggc
     9361 gctcgacaac ctcagcgctt acgacgaatc ctggttgctg gctctcgaac caagccgcgc
     9421 gctgttcgat gaaataggcc gccggcatcg aaccctgcat tgacgctgcg ctcgcgcaaa
     9481 cgcatccgca ctgccgcgca ccaccggcgc gcatccgcaa gcg
//
LOCUS       DATASH010000755         2427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5665067,
            whole genome shotgun sequence.
ACCESSION   DATASH010000755 DATASH010000000
VERSION     DATASH010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5665067"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VHV99_20190"
     CDS             complement(<1..280)
                     /locus_tag="VHV99_20190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637760.1"
                     /translation="MIHHPMTTPNMTSPLATHLVPAVATPCFVVAEDRVLHNLAVTAQ
                     ACGGVERLMPHVKTHRAAWLVRLLLAHGVGAFKASTLAEVEMVLAAGAT"
     gene            complement(342..1067)
                     /locus_tag="VHV99_20195"
     CDS             complement(342..1067)
                     /locus_tag="VHV99_20195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008165223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX3637761.1"
                     /translation="MIQFSKVNKWYGEYRALVDVTAEVKRGEVVVVCGPSGSGKSTLI
                     RTVNRLEEIQGGEITVDGTSVHQASLNLNRFRSRVGFVFQQFNLFPHLSALDNICLAP
                     LKAAGKSRVDAKRLALSLLDRVGLASKANAYPQQLSGGQQQRVAIARALAMEPPVMLF
                     DEPTSALDPEMVGEVLNVMKSLARDGMTMMCVTHEMGFAREVSDSVWFMDRGEILEVA
                     DPQTFFRSPVHPRAQAFLSDLRH"
     gene            complement(1045..1782)
                     /locus_tag="VHV99_20200"
     CDS             complement(1045..1782)
                     /locus_tag="VHV99_20200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX3637762.1"
                     /translation="MLDILIHNSHFLLLGQYPDGPIGGLALTLILSLTGLAFALPLGV
                     LLALCRVSPYKVFSVPSAALVYIVRGVPLVMLVFWSYFLVPGLIGHLVSAFTTLVVTL
                     VIYQAAYLCEIVRAGIESTPKGQSESARSLGLGYGATMRHVILPQALYNMLPSILSQC
                     VSTVKDTSIGYVISVQELTFSAQQINASLLTQPFQVFAILALTYFAVCYALTRCAHLV
                     ERRIARRRASAKMPDVRSVIDDSVFQS"
     gene            complement(1786..>2427)
                     /locus_tag="VHV99_20205"
     CDS             complement(1786..>2427)
                     /locus_tag="VHV99_20205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX3637763.1"
                     /translation="VWGLQYTALLTLTSWVLAATLGTVMATLRTMGHPFVEKLVAGYV
                     AYHQNVPMLAQLFLWYFGFPTLLPDSAQAWINAHGGEFIFASLALGLCMAAYYSEDIR
                     SGLRAVPHGQHEAARSLGLSYAKSMRYVILPQSFRIAMPPFINHTVVLFKNTSLAMAI
                     GVAELTYTVRDIENQTFRTFEIYLVGTAIYLAVSLSLMGAGAAITRRTRIAAK"
BASE COUNT          526 a          713 c          738 g          450 t
ORIGIN      
        1 gcgtggcgcc agccgccagt accatttcga cttcggcgag cgtactggcc ttgaacgcgc
       61 cgacgccatg cgccagcaat aagcgaacga gccacgcggc gcgatgagtc ttcacgtgcg
      121 gcatcaggcg ctcgacgccg ccacacgctt gcgcggtgac cgccagattg tgcaggacgc
      181 ggtcttccgc caccacgaag caaggggttg caaccgccgg caccaggtgc gtggcaagtg
      241 ggctggtcat gttgggtgtt gtcatagggt gatggatcat gacgcttccg tgccgggcgg
      301 ctgctgcact gcccggcgcg gcttcgtagg ggaaaggttg attagtgacg caggtcggac
      361 aggaatgcct gcgcgcgcgg atgcacgggc gaccggaaga aggtttgtgg gtccgctacc
      421 tcaagaatct cgccgcgatc cataaaccag acgctgtcgc tcacctcacg tgcaaagccc
      481 atctcgtggg tcacgcacat catggtcatg ccgtctcttg cgagtgactt catgacgttg
      541 agcacttcgc cgaccatctc gggatcgagc gcgctggtcg gttcgtcgaa gagcatgact
      601 ggcggctcca tggccaacgc gcgcgcaatg gcgacgcgct gctgctgtcc gcccgataac
      661 tgctgcggat atgcgttcgc cttgctcgcc aatccaacac ggtcgagcaa cgacaaggcc
      721 agtcgcttcg catccacgcg gcttttgccg gccgctttca gcggcgccag gcatatgttg
      781 tcgagtgccg acagatgagg aaacaggttg aactgctgaa acacaaagcc gacacggctg
      841 cgaaaccggt taaggttcaa cgaagcctga tggacgctcg taccgtcgac cgtgatctcg
      901 ccgccctgaa tctcctccag acggttgacg gtacggatca gcgtcgactt gcccgaccct
      961 gaaggcccac acacgaccac tacctcgcct cgcttcactt ctgcggttac gtcgacgagc
     1021 gcccggtact cgccatacca cttgttaact ttggaaaact gaatcatcga tgacgctcct
     1081 gacatctggc atttttgctg aagcacgccg gcgcgcgatg cgccgttcga cgagatgcgc
     1141 gcatcgggtg agtgcgtagc acaccgcaaa gtaggtcagc gcgagaatcg cgaaaacctg
     1201 aaacggttgt gtaagaagac tggcgttgat ctgttgggcg gaaaacgtca attcctggac
     1261 gctgatcaca tagccaatcg acgtgtcttt caccgtcgat acacattgac tgagaatgct
     1321 aggcagcatg ttgtacagtg cctgcggcag gatcacgtgg cgcatcgttg cgccgtagcc
     1381 tagtccgagc gagcgtgcgg actcagactg tcctttgggt gtcgactcga tgcccgcgcg
     1441 cacaatctcg cataaatagg ccgcctgata gatcaccagc gtgaccacga gcgtcgtgaa
     1501 cgcgctgacg agatggccga tcaatccagg caccaggaaa taactccaga acacgagcat
     1561 cacgagcggt acgccgcgga cgatatagac gagcgcggcc gaaggcacgc tgaacacctt
     1621 gtagggcgaa acccggcaca acgcgagcag tacgccaagc gggagggcaa aagcaagacc
     1681 ggttagcgag aggatcaacg tgagtgcaag tccaccgatc gggccatcgg gatattgtcc
     1741 gagcaggagg aaatgcgaat tgtggatcag tatgtcgagc atggttcatt tcgccgcgat
     1801 acgggtgcgc cgtgtaatgg cggcgcctgc gcccatcaag ctcaaagaga cggccagata
     1861 gatggccgtt ccaacgaggt agatttcgaa ggttctgaag gtctggttct cgatgtcgcg
     1921 caccgtgtac gtcagctcgg caaccccgat tgccattgcc agactggtgt tcttgaagag
     1981 caccacggtg tggttgatga aaggtggcat ggcaatgcga aagctttgcg gcaggatcac
     2041 gtatcgcatt gacttcgcat agctcagacc cagcgagcgg gctgcctcat gttgaccatg
     2101 cggcaccgcg cgcaagccgc ttcgaatatc ctcggagtag tacgcggcca tgcacaggcc
     2161 aagcgcaagc gacgcgaaga tgaattcacc accatgcgcg ttgatccatg cctgcgcgct
     2221 gtccggcagc agcgtcggga atccgaagta ccacaggaac aactgcgcga gcatgggcac
     2281 gttttgatgg tacgcaacgt agccggcaac cagcttctcg acgaatggat gacccatcgt
     2341 gcgcagcgtc gccatcaccg tgccgagtgt ggccgcgagt acccagctcg tcagcgtgag
     2401 cagggcagtg tattgcaatc cccacac
//
LOCUS       DATASH010000756         6568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6255322,
            whole genome shotgun sequence.
ACCESSION   DATASH010000756 DATASH010000000
VERSION     DATASH010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6568
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6255322"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VHV99_20210"
     CDS             complement(<1..536)
                     /locus_tag="VHV99_20210"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEX3637764.1"
                     /translation="MSTPPKIIYTLTDEAPALATYSLLPIVKAFTRSSDVIVETRDIS
                     LAGRIIAAFPDYLTAEQKGSDDLAELGGLTTRPEANIIKLPNISASVPQLKAAITELR
                     DQGYKLPSYPDVASTDAEKDVKARYDKIKGSAVNPVLREGNSDRRAPLSVKNYARKHP
                     HKMGAWSADSKSHVAHMSG"
     gene            966..3155
                     /locus_tag="VHV99_20215"
     CDS             966..3155
                     /locus_tag="VHV99_20215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEX3637765.1"
                     /translation="MQKLYVAKRPARPLLARGQRRTPSLLSAGLFVSVGLSSSTLSSS
                     GWAQAAASDASATLPAVSVSATKEATVAQADTISAGALGTRKAVDTPFSVHTVTSEEG
                     KDLFAVTSNDLFKYDPAVSISSDNALAENSMFQVRGMPIDTLNSIKVDGQTFPSWDTD
                     LPLEPFEQVELLKGLSGFMYGFGSPGGIVNFVLKRPTDDPYRSVSVGYRSAGVFSEAI
                     DLGGRFGNDNRFGYRLNLVNEDGNTAEQNGHIRRQVASLALDFRITPDLTWTADAFYQ
                     KRKTNGTLFGIGFGTGLGIPDASKVTHALTQPQNYYQTEMASFGTGLDYRLSENWHAS
                     VKYRFAKENRTNSDSFLFVQDAAGNYANTLYAAMTRYFYQNVDAMVQGTFNTGSIKHD
                     VVLGAAYQTQTAEYSNSTGWNEGYFLGIGNLYISTLLTNDEVSVGEDLYRQQRITQAA
                     LYASDTVQLTSRLSALLGVRYTQFRQYQYAPSSAVTSQYSADPVTPTFALMFKTDPYS
                     TVYASYVESLEQGGSAGNTNLNYPQTYGPLRSRQYELGFKTDHARWAANLALFRVDRG
                     YNYTNTANVFVQDGTQRFTGIDASGWLALTSDWRVMGGMMWLDTKAVDIDDPSVEGKR
                     IYGAPRFTFTGRIEYNPSYLRPLTLAFGGKYVGNQAVDAANTQFVPAYTTFDLSGRYE
                     TKIAGKDVTLRAGLNNMFNRRYWTTAWGSFVSPSPTRTAVASATMQF"
     gene            complement(3159..3458)
                     /locus_tag="VHV99_20220"
     CDS             complement(3159..3458)
                     /locus_tag="VHV99_20220"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX3637766.1"
                     /translation="MRRAQVEPQLGFDDVQGIVDAAIAGFGLAWLPCWLLTRYVQTGQ
                     LVAVMENCFRPLQEIHVMWPKSRYLALKTRSAIDALVAEIPVMVRAPTVDAPLDA"
     gene            3836..6124
                     /gene="vgrG"
                     /locus_tag="VHV99_20225"
     CDS             3836..6124
                     /gene="vgrG"
                     /locus_tag="VHV99_20225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007732484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HEX3637767.1"
                     /translation="MRKAITGRQRYLLEVLGTDKDKDISVVSFRAIEKMGQPYRIDIV
                     ATHPEQLTRDAILSHEAKFSIALDNENESRLFWGRITRFSHSGMTRDESRYEIVVEPH
                     IGCLNPRTTQTYQHKSAPEIIESILRRNGLVGHQFIFNLRRQYPVHKFRFQYQTGDWN
                     FINILMQQEGIYSYIVPGEFGDIVVFGDDIDHYIYQPTLTAAYRPMSGLETDAEFVSA
                     LRTHAELVPESFAVADYNPDQAWERLTAAANVARKDTTTFGEPYIYGTHHLDADGARW
                     EAQLRHEAAIAWQVVYEGESNVHSLCVGRMLRTDVQLPDAPDGHVMIEVTHTGARDEP
                     YRNTYQAIPSGRRFRLKIEDHKWPKISGSLSARVTSPGQYKYAYLTRQGYYVVRFDCD
                     FGEWPAGGESVPLRLAKPFAGGLQTGFHFPAVEGTEAVIEFRDGDPNKPYIAAFHHNS
                     RQLDLITNQDRLMSRNVIHTQGDNTLEFEDWEGQEHASLSTRHSGKSQLTLGHIVNGK
                     REHRGSGFELRTDGDGVVRAGGGLMLCSDIQQQAGGKQTDMAEAMNQLQARLAQAQEL
                     ADMANMAKAGIADLRAENEWLRNSVNELKEAVMLLSSPKGVAVSTPDRVSVGAGKDVN
                     MATGAGFNVSALKNIVMAAGDALSLFAHNLGIKLFAARGKVQIQAQSDELTMSALKDM
                     TITSTEGRLVLSADKELWIGAGGSYIRLTDSNIEIATPGDLFQKALTWRKDGPTSAMT
                     KAFLPYTTDLPDTGGHGSRFSG"
     gene            6189..>6568
                     /locus_tag="VHV99_20230"
     CDS             6189..>6568
                     /locus_tag="VHV99_20230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007732485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX3637768.1"
                     /translation="MPAQTHPHPAPAAAPSPVPPPAAPAELTWTFPFRDAGRKEVTDP
                     QKFYDALSLVDDGFFPLGVNGFPHGGIHFGNGSAARLDQAAGVRCIADGEIVAHKIDD
                     AHPHLQFADGKWAAYSTGFVLVRHR"
BASE COUNT         1370 a         2078 c         1907 g         1213 t
ORIGIN      
        1 ccgctcatgt gcgccacgtg cgacttcgag tccgcgctcc acgcgcccat cttgtgcgga
       61 tgcttgcgtg cgtagttctt gaccgacagc ggcgcgcgac gatccgaatt gccttcgcgc
      121 agcaccgggt tcacggcact gcctttgatc ttgtcgtagc gagccttgac gtctttttca
      181 gcgtcggtac tggccacgtc cggataggac ggcagcttgt agccctgatc gcgcagttcg
      241 gtaatcgcgg ccttcagttg cggcaccgaa gcgctgatgt tcggcagctt gatgatgttc
      301 gcttccggac gcgtggtgag tccacccagt tccgccagat cgtcggaacc cttctgttcc
      361 gcggtaaggt agtccggaaa tgcagcaatg atccggccag caagcgagat atcgcgcgtt
      421 tcaacgatca cgtcggacga gcgcgtgaac gccttgacga tcggcagcag cgagtaagtc
      481 gccagagccg gcgcttcgtc agtcagggtg tagatgatct tcggcggtgt ggacatgttt
      541 agtgctttgc taggtgaggt ggtagacaga gcgttcgtgg cgctgagccc gcgagttgcg
      601 acgagagttg cgacaaagca acaaaaacgc agcgcgaccc gcggcactac ttttacggcc
      661 cgaccgtgag tatatgcgcg tttttctcta ctcgctgcta aacaaacata ccgccacgcc
      721 gccctcaaca gggccaagcc ccgccagaca aggcttacag ccgattaccc catgacttaa
      781 gacagatgtc ttatagatga cttgaaactt cccacagatt gttaatgcga atcagatgca
      841 tcatcattag taaggttcgc aggttgaaca cacctgaacc gtccgtttaa ccacactcgc
      901 tcgaccaccg atgccatttc cgcacacttt tggtgcgcgg cggcgccacg cctaattcga
      961 acaatatgca gaaactctat gtagcaaagc ggccagcgcg ccctctcctc gcgcgcggcc
     1021 agcgacgcac gccgtcgctt ctcagcgccg ggctcttcgt cagcgtgggt ctatcgtcgt
     1081 ccacgctgtc gtcgtccgga tgggcgcagg ccgcggcgag cgacgcgtcg gccacactgc
     1141 cggccgtgag cgtgtcggcc acgaaagagg ccaccgtggc gcaggccgac accatcagcg
     1201 ccggcgcgct gggcactcgc aaggcagtgg acacgccgtt ttcggtccac accgtgacga
     1261 gcgaggaagg caaggacctg ttcgcggtaa cgtccaacga cctgttcaag tacgacccgg
     1321 ccgtgtcgat cagcagcgat aacgcgctcg ccgaaaactc gatgttccag gtccgcggca
     1381 tgccgatcga cacgctgaac agcatcaagg tggacggcca gacgttcccg tcgtgggaca
     1441 ccgacctgcc gctcgaaccg ttcgagcagg tggaattgtt gaagggcctg tccggcttca
     1501 tgtacggttt cggctcgccg ggcgggatcg tcaacttcgt gctgaaacgg ccaaccgatg
     1561 atccgtaccg cagcgtttcg gtcggctacc ggtcggctgg cgtgtttagc gaggcgatcg
     1621 atctgggcgg ccgcttcggc aacgacaatc gcttcggcta tcgcctgaac ctcgtcaacg
     1681 aagacggcaa tacggccgaa cagaacggcc acatccgccg ccaggtcgcc tcgctcgcac
     1741 tggatttccg cattacgccg gacctgacct ggacggccga cgccttctat cagaagcgca
     1801 aaaccaacgg caccctgttc ggcataggct tcggcaccgg tctgggcatt cccgacgcga
     1861 gcaaggtcac ccatgcactc acgcagccgc aaaactacta tcagaccgag atggcctcgt
     1921 tcggcacggg tctcgattac cgcctctccg agaactggca tgcgagcgta aagtaccgct
     1981 ttgccaagga aaaccgcacc aactcggaca gcttcctctt cgttcaggac gccgcgggca
     2041 actatgcaaa cacgctctat gcggcgatga cgcgttactt ctatcagaac gtcgacgcga
     2101 tggtgcaggg tacgttcaac accggcagca tcaagcatga cgtcgtgctc ggcgcggcct
     2161 accagacgca aaccgccgag tacagcaaca gcacgggctg gaacgaaggc tatttcctcg
     2221 gcatcggcaa tctgtacatc agcaccttgc tgaccaacga cgaagtcagc gtcggcgagg
     2281 atctctatcg ccagcaacgc atcacgcagg ccgcgttgta cgcgagcgac accgtgcaat
     2341 tgacgtcgcg tctgtcggcg ctgctcggtg tgcgttacac gcaattccgc cagtatcagt
     2401 acgctccgtc gagcgccgtg acgtcgcaat acagcgcgga tccagtcacg ccgactttcg
     2461 cgctgatgtt caagaccgat ccgtattcga cggtctatgc gagctatgtc gagtcgctcg
     2521 aacaaggcgg ctccgccggc aacaccaacc tcaattaccc gcagacatac gggccgttga
     2581 gaagcaggca gtacgaactc ggcttcaaga cggatcacgc caggtgggcc gccaacctcg
     2641 cgctgttccg cgtcgatcgg ggctacaact acacgaacac ggcgaacgtg ttcgtgcagg
     2701 acggcaccca gcgcttcacc ggcatcgacg cgagcggctg gctcgcgttg acgagcgact
     2761 ggcgcgtgat gggcggcatg atgtggctcg atacgaaggc tgtcgatatc gacgatccga
     2821 gcgtcgaagg caagcgtatt tatggcgcgc cgcgcttcac gttcacgggc cgtatcgaat
     2881 acaacccgtc gtatctgcgt ccgctgacgc tggcgttcgg cggcaagtat gtcggcaacc
     2941 aggcggtcga cgcagccaac acacagttcg tgccggccta caccaccttc gacctgagcg
     3001 gccgctatga gacgaaaatc gccggcaagg acgtgacgtt gcgcgccggt ctcaacaata
     3061 tgttcaaccg ccgctactgg accacggcat ggggctcctt cgtctcgcct tcgccgactc
     3121 gcacggccgt ggctagcgcg acgatgcagt tctaggcact atgcgtcgag cggggcgtcc
     3181 acggtgggcg ccctgaccat gacgggaatc tcggccacca gcgcatcgat tgccgagcgc
     3241 gtcttcaagg ccagatatcg gctcttcggc cacatcacgt ggatctcctg caagggtcga
     3301 aaacagttct ccatgaccgc gaccagctgg cccgtttgca cataacgggt cagcagccag
     3361 cacggcagcc acgccagacc aaagccggcg atcgcggcgt ccacgattcc ctgcacgtcg
     3421 tcgaagccga gttgcggctc gacctgggcg cggcggattt gcccgtcgac gtcgccccca
     3481 cctcaaccgc gaatgacgtg ttatgaggcc cgcttccagc gggctttctt tcacccacgc
     3541 gtcaagacgc ttcggcttcc ttcgcaccca tcttcagttc acgcgcttcg atgatgccgg
     3601 ccggcgcacc agaacttccc tgcacgttgc cctgaaaatt ccctgttcga gcgcggattg
     3661 gtccgattcc cgctctctga gtcaggtgtc actcttctcg caggagatat ctgcctgcgc
     3721 atcaaccacc aggcgatacg aatgactgtt tctgaagcgc aatgccttca tgcaaactgg
     3781 ataagggata acaacgatgg gcgacagact ggataagaca ggaaagcacg ctgatgtgcg
     3841 gaaagccata accgggcggc agcgctatct tctggaagta ctgggcacag ataaggacaa
     3901 ggacatttca gtggtgtcct tccgggcaat cgaaaagatg gggcaaccgt accgcattga
     3961 tatcgtcgca acacaccccg aacaactgac acgcgatgca atcctcagcc atgaggcgaa
     4021 gttcagcatt gcgctcgaca atgaaaacga atcgcgtctg ttctgggggc gtattacccg
     4081 tttctcacac tccggcatga cgagagacga gagtcgctac gaaattgttg tcgagccgca
     4141 tatcggctgt ctcaatccgc gcacgacgca gacgtatcaa cacaagagcg cgcccgagat
     4201 tatcgaatcg atcttgcgcc gcaatggcct cgttgggcat cagttcattt tcaacctgcg
     4261 acgccagtac cccgtccaca aattccggtt ccagtaccag acgggcgact ggaacttcat
     4321 caacattctt atgcagcagg aaggtatcta cagctacatc gtgccggggg aattcggcga
     4381 tatcgtcgtg ttcggagacg acatcgatca ctacatctac cagcccaccc tgactgccgc
     4441 ataccggcca atgtcggggc tggagactga tgctgaattc gtgtcggcac tgcgcacgca
     4501 tgccgagctg gtgcccgagt cgttcgctgt agccgactac aaccccgacc aggcatggga
     4561 gcgcctcacg gcagcggcca atgtcgcccg caaggacacc accacattcg gcgagccata
     4621 tatctacggc acgcatcatc tggacgctga cggtgcgcga tgggaagcac agctacgtca
     4681 tgaggcggcg attgcgtggc aagtcgtcta cgaaggtgaa agcaacgttc acagcctgtg
     4741 tgtcggcagg atgctgcgca ccgatgtgca gttgcctgac gcgcccgacg gccacgtcat
     4801 gatcgaagtc acacacaccg gcgcgcgcga cgaaccctac cgcaacactt atcaggcgat
     4861 cccctccggc cggcgctttc gcctgaagat cgaagaccac aagtggccga agattagcgg
     4921 ttcactaagc gctcgcgtca cgtcgccggg ccagtacaag tacgcgtacc tgacccggca
     4981 gggctattac gtggtgcgct tcgactgcga cttcggcgaa tggcccgcag ggggtgaaag
     5041 cgtgcctctg cgtctcgcga agccgtttgc gggcggtctg caaacgggct ttcacttccc
     5101 cgccgtcgaa gggacggagg ccgtcatcga attccgggac ggcgatccga ataagccata
     5161 catcgctgcg ttccatcaca acagccggca gctcgatctg attaccaatc aggaccggct
     5221 gatgtctcgc aacgtcattc acacgcaggg cgacaacacg ctcgagtttg aagactggga
     5281 aggtcaggag cacgccagtt tatctacccg gcactcgggc aaaagccagt tgacgttggg
     5341 gcacatcgtc aacggcaagc gcgaacaccg cggcagcggc ttcgaactgc gcaccgatgg
     5401 cgacggtgtg gtgcgtgccg gcggcggcct gatgctttgc agcgacatcc agcagcaggc
     5461 cggcggcaag cagaccgaca tggccgaagc catgaaccag ctccaggcac gcctcgcgca
     5521 ggcgcaggaa ttagccgaca tggcgaacat ggcgaaggcc ggcatagccg acctgagggc
     5581 cgaaaacgaa tggctcagga acagcgtgaa cgaactgaaa gaggccgtca tgctgctgtc
     5641 ctccccgaag ggcgtggctg tttcgacacc ggatcgggtg tcggttggcg cgggtaagga
     5701 cgtgaatatg gcgaccggtg cgggctttaa cgtcagcgct ttgaagaaca tcgtcatggc
     5761 ggcgggcgat gcattgtccc tgttcgcgca caacctcggc atcaagctgt tcgcagcacg
     5821 cggcaaggtc cagatccagg cgcaaagcga cgaactgacg atgtcggcgc tcaaggacat
     5881 gaccatcacc agcacggaag gcaggctcgt cctctccgcg gacaaggaac tctggatcgg
     5941 cgcgggcggt tcctatatcc gccttaccga tagcaacatt gagatcgcca cacccggcga
     6001 cctctttcaa aaggcgctga cgtggcgcaa agacggcccg acctccgcga tgaccaaggc
     6061 attcctgccc tatacgaccg atctgcccga tacgggcggc cacggttcca ggttctccgg
     6121 ctaaagactc cacgcgcccc gtatcccggt ttgctttcca cctgattcac ttgaccagac
     6181 atcgagacat gcctgcacag acacatccgc atcctgcacc tgcggcggcc ccctcccccg
     6241 taccgccgcc agccgccccg gccgaactaa cgtggacttt tccgttccgg gacgccggcc
     6301 gcaaggaggt caccgacccg caaaagttct acgacgcact gtcgttggtg gacgatggtt
     6361 tttttccgct cggcgtaaat ggcttcccgc acggtggcat tcatttcggc aacggctctg
     6421 cggcgcgact cgatcaggcg gccggcgtgc gctgtatcgc ggacggagaa attgtcgccc
     6481 acaagatcga tgacgctcat ccccacctgc aattcgccga tgggaaatgg gccgcgtact
     6541 caaccgggtt cgtcctcgtg cgccatcg
//
LOCUS       DATASH010000757         3189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4079743,
            whole genome shotgun sequence.
ACCESSION   DATASH010000757 DATASH010000000
VERSION     DATASH010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4079743"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="VHV99_20235"
     CDS             complement(<1..705)
                     /locus_tag="VHV99_20235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012735052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEX3637769.1"
                     /translation="MSRLIIVSNRVAPISEGGPAAGGLAVGVYDALKETGGMWFGWSG
                     DVLSSGQPQIKVEERGPVTFATIALMRRDYDQYYRGFSNATLWPAFHYRADLLQYDRH
                     DFEGYCRVNAWLARQLVPLLRDDDVIWVHDYHLIPFAQALRAAGVKNRIGFFLHIPFP
                     ASQVLLAVPPHRELVEALCSFDLLGFQTAPDLRAFCDYIVNEANGTLDVPASGPLTVH
                     AFGRSLRAAAYPIGVYP"
     gene            complement(761..1510)
                     /gene="otsB"
                     /locus_tag="VHV99_20240"
     CDS             complement(761..1510)
                     /gene="otsB"
                     /locus_tag="VHV99_20240"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEX3637770.1"
                     /translation="MQALPAVLSPSETAFFFDFDGTLVELAPTPDGVLVQPRVIVLLT
                     ELRSLTNGAVAVVSGRGIDSIDGFLGMPDLPIAGLHGAERRDANGDTQRIGFHDERLL
                     RMEQVLAQVVNEHPGMLLEIKGAALALHYRNAPDREPVAREATERLVADYPGSYVLQP
                     GKMVYEIKPKDVDKGRAMRAFLDEPPFVGRKPVFAGDDLTDEKGFAVVNERGGWSIKV
                     GAGDTTAHTRVESVTALLDWLESIVAAARKA"
     gene            1986..2555
                     /locus_tag="VHV99_20245"
     CDS             1986..2555
                     /locus_tag="VHV99_20245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX3637771.1"
                     /translation="MVGALATLGGCTHPAEPWQLTNVTGHLPDLDFTLTGDDGHAVTG
                     DTFKGRASLVYFGYTHCPDVCPETMGRLMQVLGKLGPDAQKVRILFITVDPARDTPKA
                     LHDYVGAFDSQHAEGLTGTDWQIESLAKRYRVAYQMEKRDPNGDYEVTHSSAVYVFDQ
                     EGHARLLATDHDTPDAIAQDLRRIIDDRS"
     gene            2574..3035
                     /locus_tag="VHV99_20250"
     CDS             2574..3035
                     /locus_tag="VHV99_20250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEX3637772.1"
                     /translation="MSIKIKTFAALSCTLALSFGCASAAFAAGANAISVKDAWVRWLP
                     NNLPAAGYVTLVNAGDKPVDLVDISSNDYGDAMLHQTVSNGSTQKMVMVDKLTVPAHG
                     QVSIAPGGYHVMLEDAKHKVAPGDTVHLTLKFSDGETLDTPFAVKSPAQTK"
     gene            3053..>3189
                     /locus_tag="VHV99_20255"
     CDS             3053..>3189
                     /locus_tag="VHV99_20255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX3637773.1"
                     /translation="MNLLYWLDPWEFSPTVVIALLIPAILFVRGARKARLSLARRLSF
                     W"
BASE COUNT          616 a         1084 c          930 g          559 t
ORIGIN      
        1 cggatagaca ccgatcggat aagccgccgc gcgcaagctg cggccgaatg catgcacggt
       61 cagcgggccg ctggcgggca cgtcgagcgt gccgttcgcc tcgttgacga tatagtcgca
      121 gaacgcccgc aaatccggcg cggtctggaa accgagcaga tcgaacgaac agagcgcttc
      181 gaccagctcg cgatgcggcg gcaccgccag caatacctgc gaagccggaa acggaatatg
      241 cagaaagaaa ccgatgcgat ttttcacgcc ggccgcgcgc aacgcctgag cgaacggaat
      301 caggtgatag tcgtggaccc agatgacgtc gtcgtcgcgc aggagcggca cgagttgccg
      361 ggcgagccac gcattgacgc ggcaatagcc ttcgaaatcg tgccggtcgt actgcagcag
      421 atcggcccga tagtggaacg cgggccatag cgtggcgttc gaaaaaccgc ggtagtactg
      481 gtcgtagtcg cgacgcatca gcgcgatggt cgcgaaggtc accggcccgc gctcttcaac
      541 cttgatctgc ggctgacccg agctgagcac gtcgccgctc cagccgaacc acatgccgcc
      601 ggtttccttc agcgcgtcgt acacgccgac cgccagaccg cccgccgccg ggccgccctc
      661 cgagatcggc gcaacccggt tcgatacgat gatcagtcgg ctcatgaagc gcgattcccg
      721 atgcagtgcg tgttgtacgt tgagcgatac ggcgtatcca tcatgccttg cgcgccgccg
      781 cgacaatcga ttccagccag tcgagcaacg ccgtgaccga ctcgacgcgc gtatgcgccg
      841 tcgtgtcgcc cgcgcccacc ttgatcgacc agccgccgcg ctcgttgacg acggcaaagc
      901 ctttctcgtc ggtcagatcg tcgccggcaa acaccggctt gcggccaaca aacggcggct
      961 cgtcgaggaa cgcgcgcatc gctcggccct tgtcgacgtc cttcggtttg atttcgtaga
     1021 ccatcttgcc cggctgcaac acatacgaac ccgggtagtc ggccaccagc cgctcggtcg
     1081 cctcgcgcgc cactggctcg cggtcgggcg cgttgcggta atgcagcgcc agcgccgcgc
     1141 ccttgatctc gagcagcatg ccgggatgtt cgttgacgac ctgggcgagc acctgctcca
     1201 tgcgcaacag gcgctcgtca tgaaaaccaa tgcgctgcgt gtcaccattc gcgtcgcgcc
     1261 gctcggcgcc gtgcagaccc gcgatcggca gatcgggcat gccgaggaag ccgtcaatgc
     1321 tatcgatgcc gcggcccgac acgaccgcca cggcgccgtt cgtcaggctc cgcaactcgg
     1381 tcagcaacac gatgacgcgc ggttgcacca gcacgccgtc cggcgtgggc gcgagttcga
     1441 cgagcgtgcc gtcgaagtcg aagaaaaatg ccgtctcgct gggagacaga acagccggaa
     1501 gtgcttgcat gggttaaatt tgcctttcag ccttctgcgg ccggaaaagt gtgcgcatct
     1561 taccgcgccg ccgtcaattt ttcgagaaag taagcctgcc cacaagcctg gccgggcatt
     1621 acggccgaca ccactcacaa ttacgttcaa caggacaaca atccggcttc agccagcttt
     1681 caaccgcctg cgtcaaattg actcgcaatc cgtgcgcctg cgggcgcctg gcgacgtcat
     1741 cggagatttt gcgatacagt cgcagccacg cagactattg cagtgacgac tctatttgca
     1801 cactacaacg ggtcgcgcgc tttgcccgaa ccgcgtcgcg tccgttcact ggcagcgtca
     1861 cgcgccgctc ttcaatcgag tcattgaacc ctgctttgtt cccaatcatc tcgtcccggc
     1921 acccgcagcc gaacctctcg ccaccgcgcc agccgtggac gaggcgcctg gttgcgttgt
     1981 cagtcatggt gggcgcattg gccacactag gcggctgcac gcatcccgcc gaaccgtggc
     2041 agctgaccaa tgtgaccggc cacttgccgg acctcgattt cacgctgacc ggcgacgacg
     2101 gccatgccgt caccggcgat acgttcaaag gccgcgcgtc gctcgtctac ttcggctaca
     2161 cgcattgtcc agacgtctgc cctgaaacga tgggccgcct gatgcaagtg ctcggcaaac
     2221 tcggcccgga cgcgcaaaaa gtgcgcattc tgttcatcac cgtcgacccc gcgcgcgaca
     2281 cacccaaagc cttgcacgat tacgtcggcg ccttcgactc acaacacgcc gaaggcctca
     2341 ccggcaccga ctggcagatc gagtcgctcg ccaagcgtta tcgggttgcc tatcagatgg
     2401 aaaaacgcga ccccaacggc gactacgaag tcacccatag ctcggccgtc tacgtgttcg
     2461 atcaggaagg tcacgcgcgc ctgctcgcca ccgatcacga tactcccgat gcgatcgcac
     2521 aagacctgcg ccgtatcatt gatgaccgtt cctgaccttt cccgagttca tccatgtcga
     2581 tcaagatcaa aacgttcgcc gcactgagct gcaccctcgc cctctccttc ggttgcgcgt
     2641 cggcagcgtt tgccgccggt gctaacgcga tcagcgtcaa agacgcgtgg gtccgctggc
     2701 tgccgaacaa cctccccgcc gccggctacg tgacgctggt gaacgcgggc gacaagccgg
     2761 tcgacctcgt cgatatttcc agcaacgact acggcgacgc gatgctgcat caaacggtat
     2821 cgaacggctc gacgcaaaaa atggtgatgg tcgacaagct gacggtgccc gcacacggcc
     2881 aggtatcaat cgcgccgggc ggctatcacg tgatgcttga agacgcaaag cacaaggtcg
     2941 caccgggcga caccgtgcat ctgacgctga agttctcaga cggcgaaacg ctcgatacgc
     3001 cgttcgccgt caagtcgcct gcccaaacca agtaacccgg tagcgctccg cgatgaatct
     3061 gctctactgg ctcgacccct gggagttttc gccgaccgtc gtaatcgcgc tgctgatccc
     3121 cgcgatcctg ttcgtgcgcg gcgcccgcaa ggcgcggctg tcgctcgcgc ggcgtctgtc
     3181 gttctggtt
//
LOCUS       DATASH010000758         6736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3292230,
            whole genome shotgun sequence.
ACCESSION   DATASH010000758 DATASH010000000
VERSION     DATASH010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6736
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3292230"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VHV99_20260"
     CDS             complement(<1..569)
                     /locus_tag="VHV99_20260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007582314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HEX3637774.1"
                     /translation="MKWFDRMTVFRKLLLAFAVVIGFCVVIGGSSLKTLSSMHGITDA
                     ICNQHMNGLYWMEEANRHKIDSDLAAANLGYATDDAGRQKLKDGIVASLKEMHGAYDQ
                     YRATIATSKGQELFDEVLRKSAVWEAIVHQQIGLEPIPAGVDNNELVRRAITSSEALR
                     GRITALIDYRRQQANEAQQEASADYQHMR"
     gene            complement(981..1595)
                     /locus_tag="VHV99_20265"
     CDS             complement(981..1595)
                     /locus_tag="VHV99_20265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007746835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2239 family protein"
                     /protein_id="HEX3637775.1"
                     /translation="MTPHPATCTAFEGHRRIASGSLSDVALAVRAVLARGEQAPVLIF
                     DDITARPVELDLRGTPEEILGRLANQAPGEQPAPTAEAEEEDIPRGRGRPKLGVVARE
                     VTLLPRHWSWLNGQSGGASVALRKLVEAARSASEDKDRKRAAQEAVYRFMTALAGNLP
                     GYEDATRALYASDRTRFDQLIAAWPEDVRSHTLRLAEAALVTHL"
     gene            complement(1692..2633)
                     /locus_tag="VHV99_20270"
                     /pseudo
     CDS             complement(1692..2633)
                     /locus_tag="VHV99_20270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436846.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
     gene            complement(2789..3490)
                     /locus_tag="VHV99_20275"
     CDS             complement(2789..3490)
                     /locus_tag="VHV99_20275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX3637776.1"
                     /translation="MKVLIFGATGMVGQGVLRECLRAADVEAVLTIGRTGTGQLDPRL
                     VELIQPDLMDYRAAEPSLKGFDACFFCLGVSSAGLSEADYARLTYDLTLAAAQTLARL
                     NPQMTFVYVSGAGTDSTERGRNMWARVKGKTENALRRLPFKAVYLFRPGVIEPLNGAR
                     SKTRAYRLFYTAARPFLSILRALLPNHILSTEDIGLAMLTVARHGADKAVLEVADIRA
                     LSRLAAEPALDLPAR"
     gene            complement(3538..4413)
                     /locus_tag="VHV99_20280"
     CDS             complement(3538..4413)
                     /locus_tag="VHV99_20280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3637777.1"
                     /translation="MSKVWLVTGSARGLGREIVEAALRAGDRVVATARDPRQLVELVT
                     RYGEQVRAVALDVTDPAAARHAVQAAVDAFGRLDVVVNNAGFGHLAPFEQTTEDDFRA
                     QIDTNFHGVVNVTRAALPVLRGQRAGHIIQISSVGGRVGIAGLSAYQAAKWAVGGFTE
                     VISQELAPFGVHVTALEPGGMKTEWGTEASRVTPELLPDYVPSVGAVADMLSQHFGHE
                     TGDPAKVAQVVLKLAYHDALPAHLLLGSDALHYAGQGDKARAASEQAWRAVSIATDFS
                     ATAPLPAFPAATAKA"
     gene            complement(4469..5041)
                     /locus_tag="VHV99_20285"
     CDS             complement(4469..5041)
                     /locus_tag="VHV99_20285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX3637778.1"
                     /translation="MARPKSEDKRNAILAAAAEIIAEQGLGAPTARIAKLAGVAEGTL
                     FTYFDNKDELLNELYLEIKAELREVMMTTYPKQASVRERAQYVWNKFVDWGVAQPQKR
                     RAMAQLSVSERLTDCTRATGMESFADINAMIQASVASGALRDHPPAFLSAIMAALAET
                     TMDFIAREPAAADRYRKAGFEAFWHAIVQK"
     gene            5483..>6736
                     /locus_tag="VHV99_20290"
     CDS             5483..>6736
                     /locus_tag="VHV99_20290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX3637779.1"
                     /translation="MTLKHFTIKAKLIAGFGMLSFVVVAVSCLSLKALSDSTDGFSNF
                     VHSINARADMAVQVRTAVDRRAIAARNLVLVTKPQDLEIEKADVTRAHEDVQTDLKKL
                     NDMIASATDTTEKARSLVAEINRVESAYGPVALNIVNLALTNKHDEAIVELDDRCRPL
                     LAALIRATNAYADYTHGRQEQIVNEYEAHYESQRNLLIAICLIALGLAAGACVVITRA
                     VTGPLRLAIDVARTVSEGDLRTRITVEGNDEISKLLSALREMNERLTAIVGRVRDSST
                     SIAGAARQIAAGNMDLSQRTEQQAASLQETASSMEELTSTVKQNADNAQQGSMLAANA
                     SAVAQKGSKVVGQVVNTMHDISDSSTKIADITGIIEGIAFQTNILALNAAVEAARAGE
                     QGRGFAVVASEVRSLAQRSSSAAKEI"
BASE COUNT         1367 a         2089 c         2010 g         1270 t
ORIGIN      
        1 atgcgcatgt gctgatagtc ggcgctcgcc tcctgctgtg cttcgttggc ttgctgacgg
       61 cgatagtcga tcagcgccgt gatgcgaccg cgcagcgcct cgctcgacgt aatcgcgcgg
      121 cgcacgagtt cgttgttgtc gacgccggcc ggaatcggtt cgagaccgat ctgctgatgg
      181 acgatggcct cccacacggc cgacttgcgc agcacttcgt cgaacaactc ctgacccttg
      241 ctggtcgcaa tggtcgcgcg atactgatcg taggcgccgt gcatctcctt gagcgatgcg
      301 acgatgccgt ccttcaactt ttgtcggccg gcatcgtcgg ttgcgtagcc gagattcgcg
      361 gcggcaagat cggaatcgat cttgtggcgg ttggcctctt ccatccagta gaggccgttc
      421 atgtgctggt tgcaaatggc gtcggtaatg ccatgcatcg acgacaacgt cttcagcgaa
      481 ctcccgccga tcacgacgca aaatccgatc acaacggcaa aagccaggag caacttcctg
      541 aatacggtca tgcggtcgaa ccacttcatc tcaccatcct tcaaaattcc tgacgggacg
      601 atcgcgccgc cgcgccatcg cgaacatttc gcgatttctc agatacgtgg cgacgctccc
      661 acctctttta cggcggccgt agcagcgacc tgaagcaaca aatggcggga ataaccggga
      721 caagatgacg taaggaatgc ctggcggagg aggccggttc gctacatcaa tgcgctttgc
      781 ctagctggcg cactgattgg gatttgtctg atatatcggg gcgagtcgaa ctcttaaact
      841 ttgtttgctg cgtttcaccc catgagtaag cgcatgcgat gttgttgtac tctccgactt
      901 tccgctcacg ctccccggcg tgagcggttt tttttgtcct tacacttcgt ggcctcgcac
      961 tcgtcaaagt acgctcgact ttacagatgc gtgacgagtg cagcttcggc aaggcggagc
     1021 gtgtgactgc gcacatcctc cggccatgct gcgatgagct gatcgaaccg cgttctatcg
     1081 ctggcataga gcgcgcgcgt tgcatcttca taaccaggca gattgccagc gagcgcggtc
     1141 atgaagcgat acaccgcttc ttgcgctgcg cgtttgcggt ccttgtcctc gctcgccgag
     1201 cgcgccgctt cgaccagttt gcgcagcgca accgaggcgc cgcccgattg cccgttgagc
     1261 caactccaat gacgcggcag cagcgtcacc tctctcgcca ccacaccgag cttgggccgg
     1321 ccacggccgc gcgggatgtc ttcttcttca gcctcagcgg tgggcgcggg ttgctcgccg
     1381 ggcgcctggt ttgccagccg ccccagaatc tcctcaggcg tgccacgcaa atcaagttcg
     1441 accggacggg ccgtgatgtc gtcgaaaatc agtacaggtg cctgttcgcc acgcgccagc
     1501 accgctcgca cagcaagcgc tacgtcggac aaggagcccg aggcgatgcg gcgatgcccc
     1561 tcgaaggcag tgcaggtagc agggtggggc gtcatgacca actccagatc gatgaatttg
     1621 tcgaattata cccgggtata aataacgcgc aacatattac ccggataaaa ttatcccggc
     1681 ggcggatgca ccgccacgcg acgctgtcgc ggttgccgcg agaacaacat ggcgagcacg
     1741 atgatcgcca cgtcgagcgc cgcgatcagc gtgccgataa tccacgcacg atgccgccac
     1801 tccgtgtaga tatcgcgtgt cgaaagcgcg acatcgagaa tcagcggata catctcgatg
     1861 tggcggaacg cgtaccagcg ctcgacaccg tcgatcgccg cgatgccgaa gaagtctccg
     1921 ctcggctgct cggcgaagcg cgaatagttc gaggtgccga tcagactccg accaatgatc
     1981 tttggatcgt aaggacgtcg catcagcatc gtgccgtcac tcagcatcaa cgccatcgaa
     2041 ccacccgcgc ccagattcat gccggcgaac aggcgcctga agtaagtgag ccgcatcgtg
     2101 ccgaccacca cgccgccgaa gctgccgtcc ggcttcgaga cgcgtcggct caacgcaata
     2161 ctcatgtcct tgccggtgat cttgggcatg aacggatggc tgacatacag gcctacgttc
     2221 ggcgagtcgc gctgcacctt gaagtaatcg cgatcggcga gattgatggg gcgtggcgga
     2281 gaggcttgcg aatcgaacac gacgttgccg gcggcgtcga ccaccaggac cgaaccgagg
     2341 tcttttgcgc cggcggagct gtcgaacagg accatctgcc ggatcgccgg cgacaagttc
     2401 agcacaccgg gttgcttcag gccgtcgagt actgcccgca gcgacagatc gtagatctcg
     2461 agattgcgcg acacctcgcg ctcgatcagc aacgacacgt tgaaaaccga gtcctgtgca
     2521 cgccgcaacg cgtcatcgcg catctgcgcc aatacccaga ccgcaatcgc aaggatcgcg
     2581 ctggccacca cgatactgat ggcaatcacg atgttcggtc ggcgcgtcac catgcgtttt
     2641 gcattcgagc atgcgctgac ctacgcgcgt gaagaagtgg gccgctgccg gaaggcgggt
     2701 cccgtgaacc ggtagcctac cagacgccga gacgcgtcaa acggaggaga agatggggtt
     2761 tttggcggat tgcgcgaaac cagcaccctc aacgtgcggg caaatcgagc gccggttcgg
     2821 cggcgaggcg gctgagcgcg cggatatcgg cgacctcgag caccgctttg tccgcgccat
     2881 ggcgcgctac cgtcagcatt gccaggccaa tgtcttcggt gctcaaaatg tggttgggca
     2941 atagcgcgcg caggattgac aggaaaggtc tggccgctgt gtagaacagc cggtacgcac
     3001 gggtcttcga acgagcgccg ttcagcggct cgatcacgcc gggacgaaac aggtagaccg
     3061 ccttgaacgg cagtcgccgc aacgcgttct cggtcttgcc cttgacccgc gcccacatgt
     3121 tgcggccacg ttctgtgctg tcggtacccg cgccggacac gtagacgaag gtcatttgcg
     3181 gattgagccg ggcgagggtc tgcgcggccg ccagcgtcag gtcataggtg aggcgcgcat
     3241 agtcggcctc ggacaagccg gcggatgaga cgcccaaaca gaaaaagcac gcgtcaaagc
     3301 ccttcagcga aggctctgcc gcgcggtagt ccatcagatc cggctggatc agctcgacga
     3361 gccgcggatc gagctggccg gtgcctgtgc ggccaatcgt caggaccgcc tcgacgtcgg
     3421 ccgcgcgcag gcattcgcgc aatacacctt gtccgaccat gccggtcgcg ccaaatatca
     3481 gaaccttcat gatgcgtgct ccatgatcgc gctcggccag cgggcgttga tttgtgttca
     3541 ggctttcgcc gtagcagccg gaaacgccgg caacggcgcc gttgcggaga agtcggtcgc
     3601 gatgctcacc gctcgccacg cttgctcgct tgcagcgcgg gccttgtcgc cttggccggc
     3661 gtagtgcaat gcgtcactgc cgagtaagag atgggccggc aatgcatcgt gataagcgag
     3721 cttcagtacc acttgcgcca ccttcgcggg atcgccggtt tcgtggccga agtgctgaga
     3781 cagcatgtcc gctaccgcgc cgaccgacgg cacatagtcc ggcagcaact cgggcgtcac
     3841 gcggctcgcc tcggtgcccc actctgtttt catgccacca ggttcgagcg cggtcacgtg
     3901 gacgccgaac ggcgcgagtt cctggctgat cacctcggta aagccgccga ccgcccactt
     3961 cgccgcctga taggcgctca aaccagcgat cccgacccgg ccgcccaccg acgaaatctg
     4021 gatgatgtgg ccggctcgct gcccccgcag caccggcaac gccgcgcgcg tcacattgac
     4081 gacgccatgg aagttcgtgt cgatctgcgc gcggaaatcg tcctcggtgg tttgctcgaa
     4141 cggtgcgagg tggccgaagc ccgcgttgtt gaccaccaca tcgagccggc cgaatgcgtc
     4201 tacagcggct tgcactgcat ggcgtgccgc cgccggatcc gtgacatcca gcgcgaccgc
     4261 ccgcacctgc tcgccgtagc gcgtaaccag ctccaccaac tgacgcggat cgcgcgccgt
     4321 agccaccacg cggtcgccgg cacgtaacgc cgcttcgaca atttcacgcc ccaacccacg
     4381 ggcactcccg gtaaccaacc agactttcga catagtgtgc tccttacatt taaatgagca
     4441 agcaatcact cattaattag acaaaaaatt atttctggac gatcgcgtgc cagaacgctt
     4501 caaagccggc cttgcggtac cggtcggcgg cggccggttc gcgagcgatg aaatccatgg
     4561 tggtttcggc cagcgccgcc atgatcgccg acaggaaagc cggcgggtga tcacgcaacg
     4621 cgccgctcgc cacgcttgcc tgaatcattg cgttgatgtc cgcgaaggac tccatgcccg
     4681 tcgcgcgcgt gcagtcggtc agccgctccg acacggacag ttgcgccatt gcccggcgct
     4741 tttgcggctg ggccacgccc cagtcgacaa acttgttcca tacgtactgg gcgcgctcac
     4801 gcacgctggc ttgcttcggg taggtggtca tcatcacttc gcgcaactcc gccttgatct
     4861 ccaggtagag ctcgttcagc agctcgtcct tgttgtcgaa gtatgtgaac agcgttcctt
     4921 cagccacacc cgcgagtttt gcgatgcgcg cggtgggcgc gcccaggccc tgctcggcga
     4981 tgatctctgc ggcagctgca aggatggcgt tgcgtttgtc ttcgcttttt ggacgggcca
     5041 tacgacggtt cgctttgaca accaataata aatgagtgat tgctcaatca tagccgtgga
     5101 cgtccgcttg ctcaagccat ttttgttgct gcaggcccgg cgatggctat ttgctcggca
     5161 tccgccctaa agtgaaaaag gaaatgcttt caccatccga ctaaatatat tcaaagttat
     5221 gcgaaaagac gcgttcttcg cgcatcgcgc gcaaggttgg taaagaaata ctcgcgattt
     5281 cttaagtagc aagaatccag tccgataacc aataaatggg atacccattt gttgcctcaa
     5341 tttcggccat gcgtccgata ggagccacga agggcaaacc gcgcgggctg ccgcgatgac
     5401 cggcagccag atagacactc ccacgcccgg acctgagagt ccattgcgaa cgccttaacg
     5461 tttcgttatt ggagaaaaaa tcatgacact caagcatttc accatcaagg ccaaactgat
     5521 tgccggcttc ggcatgttgt ctttcgttgt cgtcgcggtt tcctgcctct ccctgaaagc
     5581 gctgagcgat tcgaccgatg gcttttccaa ctttgtacac agcatcaacg cacgcgccga
     5641 catggcggta caggttcgaa ccgccgtgga ccgcagggcg atcgcggcgc gcaacctcgt
     5701 gctcgtgacc aaacctcagg atctcgaaat agagaaagcg gacgtgaccc gcgcgcacga
     5761 agacgtgcaa actgacctga aaaagctcaa cgacatgatc gcgagtgcga ccgacacgac
     5821 ggagaaagcg cgcagcctcg tcgcggaaat caatcgggtg gagtcggctt atgggccggt
     5881 tgctctcaac atcgtcaatc tcgccctcac caacaagcat gacgaagcga tcgtcgagtt
     5941 ggacgatcgt tgccggccgc tgctcgccgc gttgattcgg gcgaccaacg cgtacgccga
     6001 ctacacacat ggacgtcagg aacagatcgt caacgaatac gaggcccact acgagagcca
     6061 gcgtaatctg ctgatcgcga tttgcctcat cgcattaggg ctcgccgccg gtgcgtgcgt
     6121 ggtgatcacg cgtgcggtga ccggaccgct gcgtttagcg atcgacgttg cgcgcaccgt
     6181 gtcagaaggc gatctgcgca cgcggatcac cgtcgaaggc aacgacgaaa tcagcaaact
     6241 gttgagcgcc ctgcgcgaga tgaacgaacg gttgacggcg attgtcggac gggtgcgcga
     6301 cagcagcacc agcatcgccg gcgcggcccg tcagatcgcg gcgggaaata tggacctgag
     6361 ccagcgtact gagcagcagg ctgcgtcgtt gcaggaaacc gcgtccagca tggaggaact
     6421 cacctccacg gttaagcaga acgccgacaa cgcgcagcaa ggcagcatgc tggccgcgaa
     6481 tgcatcggca gtggcgcaaa agggtagcaa ggtcgtcggt caggtggtga acacgatgca
     6541 tgacatcagc gatagttcga ccaagatcgc ggacatcacg gggatcatcg aaggcattgc
     6601 gttccagacg aacatcctcg cgttgaatgc ggccgtcgaa gccgcacggg caggcgaaca
     6661 aggacggggt ttcgcggtgg tcgcgagcga ggtcagaagc ctggctcagc gttcttcaag
     6721 cgcggcaaag gagatc
//
LOCUS       DATASH010000759         4024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4871904,
            whole genome shotgun sequence.
ACCESSION   DATASH010000759 DATASH010000000
VERSION     DATASH010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4871904"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="VHV99_20295"
     CDS             complement(<1..503)
                     /locus_tag="VHV99_20295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3637780.1"
                     /translation="MPTPADKSNSKESEGVAVIDRACAILFAFRPDDVALTLAELAAR
                     TGLYKSTLLRLAGSLIQHRLLLRLDDGRYQLGSATFMLGALYQRSQNVGDILVPLMRE
                     LAETSGESVSFYVRDEAVRVCLHRVDSTHAVRFHVREGDVLPLESGSGGHALLAFGGM
                     PGEPYEK"
     gene            806..1684
                     /locus_tag="VHV99_20300"
     CDS             806..1684
                     /locus_tag="VHV99_20300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3637781.1"
                     /translation="MRPYLPLNALRAFESSARHLSFTRAALELNVTQAAVSQQVRALE
                     ERLGTALFKRLPRGLAMTDEGLALRPVLTDAFDRIEAVLKQFDGGHFHEVLTVGVVGT
                     FAVGWLMPRLKSFRDAHPFVELRLLTNNNLVDLATEGLDFAIRFGDGTWPGSLATRLL
                     DAPLAILCAPEIAERLSDPMDLADETLLRSYRVDDWMAWFTAAGIPPRPIRGPVFDSS
                     RLMVEAAMQGAGIALAPALMFDHEINTGRLVRPFEVEVHAGSYWLTWLKGKAMAPAML
                     LFSQWIMKEAALHPPG"
     gene            complement(1681..1935)
                     /locus_tag="VHV99_20305"
     CDS             complement(1681..1935)
                     /locus_tag="VHV99_20305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637782.1"
                     /translation="MVAAEKTLHWAVDKWLAPTPSMPARVVRFCHSASQRQRYVCVEA
                     LRPGGLLSIFFFRHDDGSWNVFPPQAERPAMNGHRRASVC"
     gene            complement(1997..2500)
                     /locus_tag="VHV99_20310"
     CDS             complement(1997..2500)
                     /locus_tag="VHV99_20310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lecithin retinol acyltransferase family protein"
                     /protein_id="HEX3637783.1"
                     /translation="MNTNPLPASSEDRDACRDEASTIDLPIGAHLVTQRRGYEHHGIY
                     VGNGHVVHYAGFAGSAHRGPVEEVELTRFAAGHPLTIRSTPSARYVGDEAVRRARSRL
                     GENRYRLLTNNCEHFCAWCLLGESHSEQVHCCLRHPRTGMHALVCLVKAFVESVAKDG
                     HVTAQLA"
     gene            2960..3247
                     /locus_tag="VHV99_20315"
     CDS             2960..3247
                     /locus_tag="VHV99_20315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637784.1"
                     /translation="MASITVNDLSLNLALDRKAMAAIRGGGGAPWVYGWIQPYVRETP
                     SIGPVVNLYDISNNFYANQMINTFQSVDVRNTGANSNINVSPDARSRNDTV"
     gene            3253..4023
                     /locus_tag="VHV99_20320"
     CDS             3253..4023
                     /locus_tag="VHV99_20320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEX3637785.1"
                     /translation="MTCPSQFRWMSSARSDAGRIREINEDACLAQPEPGRWAVADGMG
                     GHAVGDLASRLVIDTLSRLAPPLAMKTGIADARARLQMVNRQLRDEAARRQVQRIGST
                     VIVLLACDRFCGYLWAGDSRLYLYREGQLRQLTRDHSQVEQLKSLGVITDEEARHHPA
                     QHMITRAVGATDVLELDDDAIEVADGDVFLLCSDGLSNEISDADILAVLTSVACERAS
                     DELVELAIARGGRDNVTAVVVQAEDPYASDKTLLNPAP"
BASE COUNT          763 a         1226 c         1275 g          760 t
ORIGIN      
        1 atcttttcgt acggctcgcc cggcatgccg ccgaatgcca gcaaggcatg gccgccggag
       61 ccgctttcca gcggcagtac gtccccttct cgcacgtgaa aacgcactgc atgcgtcgag
      121 tccacacgat gcaggcatac gcgcaccgcc tcgtcgcgca cgtaaaaaga cacgctttcg
      181 ccgctcgttt ccgcaagctc gcgcatcagc ggcaccagaa tatcgccgac gttttggctg
      241 cgctgataca gcgcgccgag catgaaggtt gcggagccga gttggtagcg gccgtcgtcg
      301 agacgcagca gcaggcggtg ctggatcagc gagcccgcga gccgtaacaa cgtgctcttg
      361 tagagccccg tgcgtgccgc gagttcagcg agtgtgagcg cgacgtcgtc cggccggaag
      421 gcgaacagaa tcgcgcaagc acgatcgatg acggcaacgc cctcgctttc ctttgagttt
      481 gatttgtccg caggcgttgg catgagttgg ctgggtaagc gttgcttcat atcgaagtgg
      541 aaattttacg ctatgacgcg gggctgtcat acctgacata cctgggcaac ggtccttcgt
      601 aggggaaccg gccgggaaac gcggagctga cggcgaaacg ctgcaaaccc agcaaacacg
      661 cacgacggat gggcgtcgcg cccgcgaact tccgtcttgt gatcatcgac gctccttatg
      721 tgaagccgca acgaccggtt ttccggcgcc cgctgacaaa cgatgataag ttagtcgact
      781 cccaagaaaa tcttatgcct tcgccatgag accgtatctc ccgttgaatg cgttgcgcgc
      841 cttcgaatcg tccgcgcggc acctgagctt tacgcgcgcc gcgctcgaat tgaacgtcac
      901 ccaggcggcc gtgagccagc aggtcagagc gctggaagaa cggctgggca ccgcgctctt
      961 caagcgtctg ccccgcggac tggccatgac cgacgaaggc ctggcgctac gccccgtcct
     1021 cacggatgcg ttcgatcgca tcgaagccgt gctcaagcag ttcgacggcg gccactttca
     1081 cgaggtattg acggtcggcg tggtgggcac cttcgcggtg ggctggttga tgccgcggct
     1141 caaatcgttt cgcgacgcgc atccgtttgt cgagttgcgg ctgctgacga ataacaatct
     1201 ggtcgatctc gccacggaag gcctcgactt cgcgatccgt ttcggcgacg gcacctggcc
     1261 gggttcgctc gccacgcggc tgctcgatgc gccgctcgcg attctgtgcg cgccggagat
     1321 cgccgaacgt ctgtccgatc caatggatct cgccgacgaa accttgttgc ggtcctatcg
     1381 cgtcgacgac tggatggcgt ggttcacggc cgccggcatc ccgccccggc cgattcgcgg
     1441 accggtgttc gattcatcgc ggctgatggt ggaagcggcc atgcaaggcg cgggtatcgc
     1501 gctggcgccg gctttgatgt tcgatcacga gatcaatacg ggccggctgg tgcgcccgtt
     1561 cgaggtggaa gtgcatgccg gcagttactg gctgacgtgg ctgaagggca aagcgatggc
     1621 gccggccatg ctgctgttca gtcagtggat catgaaggaa gcggccttgc acccacccgg
     1681 ctagcaaacc gacgcgcggc gatggccgtt catcgcgggc cgctcggctt gcggcggaaa
     1741 cacgttccat gagccgtcgt cgtgacggaa gaagaagatc gacagcaagc cgcctggccg
     1801 cagtgcttcc acgcaaacgt agcgttgccg ttgcgacgcg ctgtggcaaa agcgcacgac
     1861 acgcgccggc atcgaggggg tcggtgcgag ccatttatcg acggcccagt gcaaggtctt
     1921 ttctgcggca accatgatgt gctcactgat ccttgaatga atgaattcgt gaacgcttga
     1981 actcgttccc gctcgctcag gcgagttggg cggtaacgtg accatccttc gcgacacttt
     2041 cgacaaaagc cttcaccaga cacaccagcg catgcatgcc ggtgcgggga tgcctcaaac
     2101 agcaatgcac ctgttcgctg tggctctcgc caagcaggca ccacgcacaa aagtgctcgc
     2161 agttgttcgt cagcaggcgg tagcggtttt cgccgaggcg ggagcgcgcg cggcgcacgg
     2221 cttcatcgcc gacgtagcgg gcgctcggcg tcgagcggat cgtcaatgga tgaccggcgg
     2281 cgaagcgggt taattccact tcctccaccg ggccgcgatg cgccgaaccc gcaaagccgg
     2341 cgtaatgaac cacgtggcca ttgccgacgt agatgccgtg gtgttcgtag ccgcgccgtt
     2401 gcgtgacgag atgcgcgccg atcggcaggt cgatggtcga tgcttcgtca cggcatgcgt
     2461 cgcggtcttc gctacttgcc ggaagtgggt tcgtgttcat ggctggcctc gcttcaatat
     2521 ccaacactgt tgcgagagct tttgcatcat ccgggccatg tgcggaaagg cctttattta
     2581 aagggctgac aggaatggcg agatgttttg aatggggcaa gtgtccgctg tgagccgaca
     2641 gaaaacaaac ccatgtgttg gccttggccg atcatttggc gcgctgactg agcggccccg
     2701 agcggctcgg ttaaggtccg tgatctgagg ccaatgtgtt gggtcccgca ccgatgcgca
     2761 cgcgcggatg ttgggtgcgt tccgacctct gcacggccac gtgaacgcgc gcggcatgca
     2821 cagccacccg gaatcgccgc gctatcaacg cgctacgaga aaacgcgcgt gcgagccgca
     2881 aattggcatg gcacttgcac cattacgtcc attccttttc aatcgcacgc ccgacgcacg
     2941 cggcaaaccg gagaacgaca tggcttctat cacagtgaac gatctttcgc taaacctcgc
     3001 gctcgaccgt aaggcgatgg cggcgatccg cggcggcggc ggtgcgccgt gggtgtacgg
     3061 ctggattcag ccctatgtgc gggaaacgcc gagcatcggt ccggtcgtga acctgtacga
     3121 catctcgaac aacttctatg ctaaccagat gatcaacacg ttccagtcgg tggatgtgcg
     3181 caacaccggc gcgaactcga acatcaacgt gtcccctgac gcacgcagca gaaacgatac
     3241 ggtgtagccc ttgtgacctg tccgagccag tttcgatgga tgtcgtccgc gcgttccgac
     3301 gccggacgca tccgtgaaat caacgaagat gcctgcctcg cccagcccga acccggccgc
     3361 tgggccgtcg ccgatggcat gggtgggcac gcggtcggcg atctggcgag ccgcctcgtg
     3421 atcgatacac tgagccggct cgcgccgccg ctcgccatga aaaccggtat cgccgacgcg
     3481 cgcgcacggc tgcaaatggt caatcgccaa ctgcgggatg aagccgcgcg ccgccaggtg
     3541 caacggatcg gcagcacggt gatcgtgctg ctcgcctgcg accgtttctg cggctacttg
     3601 tgggccggcg acagtcgcct ttatctgtat cgcgaagggc aattgcggca actgacgcgc
     3661 gatcatagcc aggtggaaca actcaaatcg ctcggcgtga ttaccgacga agaggcgcgc
     3721 catcacccgg cgcaacacat gatcacgcgc gcggtcggcg ccacggatgt gctcgaactc
     3781 gatgacgatg cgatcgaagt cgcggacggc gatgtctttc tgttatgcag cgatgggttg
     3841 agcaacgaga tcagcgatgc ggacatactg gcggtgctga cctcggtggc atgtgagcgc
     3901 gcgtccgatg aactggtcga gttggccatc gcgcgcggtg gacgcgacaa tgtcaccgcg
     3961 gttgtggtgc aagcggaaga tccgtatgcg tcagacaaaa ccttgttgaa cccggcgcct
     4021 tgaa
//
LOCUS       DATASH010000760         4286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6255845,
            whole genome shotgun sequence.
ACCESSION   DATASH010000760 DATASH010000000
VERSION     DATASH010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4286
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6255845"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            431..1432
                     /gene="hemC"
                     /locus_tag="VHV99_20325"
     CDS             431..1432
                     /gene="hemC"
                     /locus_tag="VHV99_20325"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEX3637786.1"
                     /translation="MNSETPSTPPPESLVIASRESRLAMWQAEHVRFALHKLYPSCDV
                     KILGMTTRGDQILDRTLSKVGGKGLFVKELEAALADGRADLAVHSLKDVPMELPDGFT
                     LSTILEREDPRDAFVSPHYASLSELPEGSIVGTSSLRREAMLRMRFPHLDVRPLRGNL
                     DTRLGKLDRGDYAAIILAAAGLKRLGLESRIRASLPVEISLPAAGQGALGIEIRAGRD
                     NLAAWLAPLHHDATAAAVEAERMVSRSLGGSCEVPLAAYADWRDGVLHLRGCVSTVDG
                     TRVLRAEGSAQAADVQAALDLGSRVSAELEAQGAMDIVRELSTASGARIPPAGSPAD"
     gene            1432..3657
                     /gene="hemDX"
                     /locus_tag="VHV99_20330"
     CDS             1432..3657
                     /gene="hemDX"
                     /locus_tag="VHV99_20330"
                     /EC_number="2.1.1.107"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008350998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fused uroporphyrinogen-III synthase
                     HemD/membrane protein HemX"
                     /protein_id="HEX3637787.1"
                     /translation="MASTQGSAHSSAQGPTQSPTQRPTVVVTRPLGQSAALLALLARG
                     GFESIEFPLIDIGPVTDAAPLHAALGELYAPAPLGFALVVFVSPNAIDHAFAALSSAW
                     PLQIPIGVVGPGSVAALGRQGVTAPSHTIISPVADATVTETPTDPRYDSEALYAAIEA
                     HFGPDGLKNKRVLIVRGDGGREWLADALSAAGANVEKVAAYRRIVPEPTMRDWERIHA
                     LLAGEPHAWLLTSSEGVRNLEELAREHLTLDETLTLKHAPLVSPHPRIAEAARQAGFD
                     RITVSGAGDERIVQALGGIFGAASGQTAKQRKTEQEPDAQVAKAREHGYKASTAAPFS
                     QSSQTNQESAPTDSRPASTPAHQPAQSRMTDSNDSSKTASQSATSTPPLASNPPNTPF
                     TPYESQPPKQRGGGAGVALLWLVLLIVVVVAGVGGFMLNRKFDQRDAQFAQRLQANDT
                     QTAALRAQADQAASSVSAINTQIIQFQGKLTDAQAQSQALQQQYQDLASNRDDWTLAE
                     VEQILSSASQQLQLTGNVQLALFALQSADTRLAATTGPQIVTIRKAIAQDMDKLRATP
                     STDLTGLAIKLDTAIDQIDSLPLAGEAPITPATAHADTPRDTASIAAATGQPVWKVWL
                     QQIASGIGQQLSSLVTVRRIDNADAMLASPDQGYFVRENVKLRLLSARLSLLSRSEPA
                     LKSDLHAADTALGRYFDQSSGKVKAVRSLLQQVDQASLAVTVPDLNASLAAVHQFKRG
                     G"
     gene            3657..>4286
                     /locus_tag="VHV99_20335"
     CDS             3657..>4286
                     /locus_tag="VHV99_20335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HEX3637788.1"
                     /translation="MTMRGLIWFVLLFAAAAGLALVGVFDGGQVLLVMPPYRVDVSLN
                     LFVVALIVSFIVIYAVLRAIRSVLKMPQRVSAYRTRSRLAKANAALRDAIGHLYAGRF
                     SKAEKSARDSLIASENTCAAGLIGAKAAHEMNEYARRDEWLAKVTDSDWQDARLMATA
                     DMRADGRDADGALVALTEMQAQGGRRLRAQQIALRAQQQLKNWGEVLKLV"
BASE COUNT          767 a         1340 c         1400 g          779 t
ORIGIN      
        1 cagacgccgt atcgggcgtg gtggaagaag catccttcga taccgcgttg cggcgggggc
       61 gggcgttgcg cgccgatccg ggagacgtca cgttcaattc ctcttgaagc cagagacagg
      121 atacggaggc ggccgggcac cggagaccgg cgcgacaccg ccggccagtg cgacgttcga
      181 ctgctttgcc atgctgtatt actgcgttgc ggatgaaacc ggggatgcga agggcgcctg
      241 agcgcggcac gcacgataaa atgcgtgctg cagcgatgcc gcgcgatgcg gcaaaacctt
      301 gctgtctccg tcctgccgca tcgctcgaag cccgtgccgc gccgcttgga cacggggtcc
      361 gactgtaaat gggcaatggc gtgcgtgcta ccattgtttg ttatccatcc cgtcattcca
      421 ttgacctgca atgaactccg agacgccttc cacgccaccg cccgaaagtc tggttattgc
      481 ctcgagggaa agtcgtctag ccatgtggca ggccgagcac gtgcggtttg cgctgcacaa
      541 attatatcca tcttgcgacg tcaaaatcct cggaatgacg acacgcggcg atcagattct
      601 ggaccggacc ttatcgaagg ttggcggcaa gggcctgttc gtcaaggaac tggaagctgc
      661 gctggccgac ggccgtgccg acctggccgt gcattcgctg aaagacgttc ccatggaact
      721 gcctgacggt ttcacgctga gcacgatcct cgaacgcgaa gatccgcgtg atgccttcgt
      781 ctcgccgcac tatgcgtcgc tctcggaact gcctgaaggc agcatcgtgg ggacctccag
      841 cctgcgccgc gaagccatgt tgcgcatgcg gttccctcat ctcgacgtcc gtccgttgcg
      901 cggcaacctg gacacccggc tcggtaaact tgatcgcggc gattacgcgg caattattct
      961 cgccgccgcc gggttgaaac ggcttggcct cgagtcgcgg attcgtgcca gtttgccggt
     1021 cgaaatcagc ttgccggccg cagggcaggg cgcgctgggt atcgaaatcc gcgctggccg
     1081 cgacaatctt gccgcgtggc tcgcaccctt gcaccatgac gcgacggccg ccgccgtcga
     1141 agccgaacgg atggtgtcgc gctctctcgg cggcagttgt gaagtaccgc ttgcggccta
     1201 tgccgactgg cgcgacggcg tgctgcatct gcgcggttgt gtatcgacag tcgacggcac
     1261 gcgggtcttg cgcgccgaag gttccgccca ggccgctgac gtacaggccg cgctggatct
     1321 cggcagccgc gtgtcggcag aactggaagc gcagggcgca atggatatcg tccgtgagtt
     1381 gagcaccgcg agcggcgcgc gtattcctcc cgctggctcg cctgccgact gatggcgagc
     1441 actcaaggat ctgcccattc atcggcgcaa gggccaacgc aatcgcccac gcagcgaccg
     1501 actgttgtcg tcacgcggcc tttgggccag tcggccgcgt tgctggcgtt gctggcgcgc
     1561 ggcggtttcg agtcaatcga gtttccgctc atcgatattg gtccggtgac cgatgccgcg
     1621 ccactgcacg cggcgctcgg cgagttgtac gcacccgcgc cgcttggctt tgcgctggtc
     1681 gtgttcgtat cgccgaatgc gatcgatcac gcgtttgccg cgctcagctc cgcatggcct
     1741 ttgcagattc ccattggcgt agtcggcccc ggcagcgtgg ctgcgctcgg ccgccaaggc
     1801 gtgacggcgc caagtcatac gatcatcagc ccggtcgcgg acgcaacggt gaccgaaaca
     1861 cccactgacc cccgttacga ttccgaggcg ttgtatgcgg ctatcgaagc gcatttcgga
     1921 cccgatggtc tcaagaacaa acgtgtgctg atcgtgcgcg gcgatggcgg ccgtgaatgg
     1981 ctggcggacg cgctcagcgc agcgggcgcg aacgtggaga aggtcgcggc ataccggcgg
     2041 atcgtgccgg aaccgaccat gcgcgactgg gagcgcatcc acgcactgct tgccggcgag
     2101 ccgcatgcgt ggttgctgac aagctcggaa ggcgtgcgca accttgaaga gctggcccgc
     2161 gagcatctca ctctcgatga aacgctcacg ctcaagcatg caccgctggt gagtccgcat
     2221 ccgcgtattg ccgaagccgc gcggcaagcg ggttttgata ggattacggt gtccggcgcc
     2281 ggcgatgagc gtatcgtgca ggcgcttggt ggcatcttcg gtgcagcatc gggacagacc
     2341 gcgaaacaac gaaaaaccga acaagaaccc gacgcgcagg ttgcaaaagc gcgggaacat
     2401 ggatacaagg cctcgacggc agcgccgttc agccagtcca gtcaaaccaa ccaggagtcc
     2461 gcgccgacgg attccaggcc ggcttcaacg ccggctcacc aaccggctca atcacgcatg
     2521 actgattcga acgattcaag taaaaccgcc tctcagtccg ctacgtcgac tccgccgctg
     2581 gcgtcgaacc cgccgaacac gccgttcacg ccctacgagt cgcagccgcc gaaacagcgt
     2641 ggcggtggcg cgggcgttgc gctgctgtgg ctggtgttgc tgattgtcgt ggtggtcgcg
     2701 ggcgtgggcg gattcatgct caaccgcaag ttcgatcagc gcgacgcgca attcgcgcaa
     2761 cgtctgcagg cgaacgacac gcaaaccgcg gcgttgcgcg cacaggccga tcaggctgcc
     2821 agttccgtgt ccgccatcaa cacgcagatc atccagttcc agggcaaact caccgacgcg
     2881 caggcgcaaa gccaggcgct gcaacagcaa taccaggatc tggcgagcaa tcgcgacgac
     2941 tggacgctcg ctgaagtcga gcagatcctc tcaagcgcga gccagcagtt gcagctcacc
     3001 ggcaacgtgc agcttgcgct gttcgcgctg caaagcgcgg acacgcgtct ggctgcgacc
     3061 acgggtccgc agatcgtgac aatccgcaag gccatcgcgc aggatatgga caagctgcgc
     3121 gcaacgccgt ccaccgatct caccgggctg gccatcaagc tggacacggc aatcgatcag
     3181 atcgactccc tgccgctggc gggtgaagcg ccgatcacgc cggccacggc gcatgccgat
     3241 acgccgcgcg ataccgcttc cattgcagcc gcgaccggcc agccggtttg gaaagtgtgg
     3301 ttgcagcaga tcgccagtgg tattgggcag cagttgtcga gcttggtgac ggtgcggcgt
     3361 atcgataacg ccgacgccat gctcgcgtct cctgaccaag gttatttcgt gcgcgaaaac
     3421 gtgaagctgc gtttgttgtc ggcgcgtttg tcgttgttgt cgcgcagtga acccgcgctc
     3481 aagtcggacc ttcatgccgc ggacaccgcg ctcggccggt atttcgatca atcgtcgggc
     3541 aaggtcaagg cggtccgctc gctcctccag caagtcgatc aggcgtcgct cgcggtcacc
     3601 gtgcccgatc tgaacgcgag tctcgcggcg gttcaccagt tcaagcgagg cggatgatga
     3661 cgatgcgcgg actcatctgg tttgtactgc tgttcgctgc agctgcgggc ctggcgctcg
     3721 ttggcgtctt cgacggcggg caagtgctgc tcgtgatgcc gccgtatcgg gtggatgtat
     3781 cgctgaatct gttcgtggtg gcgctgatcg tgtcgttcat cgtgatctac gccgtgctcc
     3841 gcgccatccg cagcgtgctg aaaatgccgc aacgggtgtc ggcgtatcgc acgcgctcgc
     3901 ggctcgcgaa ggcgaacgcc gcgttgcgcg atgccatcgg ccatctgtat gccggacgtt
     3961 tttcgaaggc ggagaagtcg gcgcgcgatt ccctgatcgc cagtgaaaac acctgtgcgg
     4021 caggcctgat cggtgcgaag gccgcgcatg agatgaacga gtacgcgcgt cgcgacgaat
     4081 ggctcgcgaa ggtgacggac agcgactggc aggacgcgcg tttgatggcg accgccgaca
     4141 tgcgcgccga cggccgcgat gccgatggcg cgctggtcgc gttgactgag atgcaggcgc
     4201 agggcgggcg gcgtttgcgt gcgcagcaga tcgcgctgcg cgcgcagcag cagttgaaga
     4261 actggggcga agtgctgaag ctcgta
//
LOCUS       DATASH010000761         2885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4872057,
            whole genome shotgun sequence.
ACCESSION   DATASH010000761 DATASH010000000
VERSION     DATASH010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4872057"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="VHV99_20340"
     CDS             complement(<1..939)
                     /locus_tag="VHV99_20340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX3637789.1"
                     /translation="MTKNSDTFEIAEMGNVVLQSGATLRGARLAYKTHGTLNAARDNA
                     IVYPTWYGGQHADNEWLIGPGLALDPARYFIIVPDMLGNGLSSSPSNTPPPYNQARFP
                     HVTAYDNVKLQHRLVTETFGIETLELVTGWSMGALQAYHWGSMYPSMVKRMLPSCGSA
                     RCSPHNFVFLEGVKAALTADAEWKEGFYDKPPARGLRAMGRVYAGWGFSQAFYREKLE
                     VKALGYSSLEDFLVTFWEGGFLESDANDLLAMLWTWQHGDISANPLYNGDFDLALSSI
                     TAKTIVMPGQTDLYFPPEDSQYEVSKMPNAEYRPIPS"
     gene            complement(1159..1482)
                     /locus_tag="VHV99_20345"
     CDS             complement(1159..1482)
                     /locus_tag="VHV99_20345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637790.1"
                     /translation="MARHAGSDSSSPTTGRWLFRDKTMNAAFKESARQKRQNEPYNRI
                     AQAIEHLAVAAHQALAALTVHFHIACKSTVRVCLHVVHDIDGFVITKQMRRGHLRQAS
                     TCFNA"
     gene            complement(1610..2212)
                     /locus_tag="VHV99_20350"
     CDS             complement(1610..2212)
                     /locus_tag="VHV99_20350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HEX3637791.1"
                     /translation="MKRTAQTPAILRDLRSLKVVVIHPHDQDGEELLAQLQRIGCDVQ
                     VFWPRLDRLPSDTGLVVMAIRPEALSIDYPWLGTSTSPPVIPVVTYENPITIEAVLRL
                     NAFATIPAPVRSFGLLTAVAVALTQFKASRARERYIERLEQKQAKVRVIQQATRIVMD
                     SRGMSEEDAYQLLRSKAMLKREQIETVAGDIVKAHETLSF"
     gene            complement(2209..>2885)
                     /locus_tag="VHV99_20355"
     CDS             complement(2209..>2885)
                     /locus_tag="VHV99_20355"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter substrate-binding protein"
                     /protein_id="HEX3637792.1"
                     /translation="RGGEKVDETYLSLDAPWESYLDVAKRIKQLAPDFIFSTVVGEGI
                     PHLYRAFAQVGLDPYRTPIASHMTSEAEIAAMGNDLAEGHITSATYFQSVDTAANHDA
                     VARHQARFGEDQPTNMCWEASYFQMHLLADAIRRVGSDDPTLLLRVLPGLEFDAPQGR
                     VRIDEHNNHTYLHPLIGRVDAHCRFDIIGRAPERVKADPYVVFHSTPAWTAHAHPELA
                     RAGATR"
BASE COUNT          548 a          826 c          903 g          608 t
ORIGIN      
        1 cgacggaatg ggcctgtatt cggcgttggg catcttggat acctcgtact gactatcttc
       61 gggtggaaag tacaggtcgg tttgtcccgg catgacgatg gtttttgcag tgatgctcga
      121 aagggcgagg tcgaaatcgc cgttgtagag cgggttggcg cttatgtcgc cgtgttgcca
      181 ggtccacagc atggcgagca gatcgttggc gtcgctttcc agaaaaccgc cttcccagaa
      241 cgtcacgagg aagtcctcca gcgatgaata tccaagcgcc ttgacctcca gcttttcgcg
      301 atagaacgct tgcgaaaagc cccagccggc atagacgcgg cccatcgcgc gcagcccgcg
      361 ggcaggcggc ttgtcgtaga agccctcttt ccactctgca tccgcggtga gcgctgcctt
      421 gacgccctcg aggaacacga agttgtgcgg cgaacaccgc gccgagccgc acgagggcaa
      481 catgcgcttc accatgctgg gatacatcga gccccagtgg taggcctgca atgcgcccat
      541 tgaccagccg gtgacgagtt cgagcgtctc gatgccgaaa gtttcggtaa cgagccggtg
      601 ctgcagcttg acgttgtcat acgccgtcac atggggaaag cgggcctggt tgtagggcgg
      661 cggggtgttg ctcggcgagc tggacaagcc gttacccagc atgtccggaa cgatgatgaa
      721 atagcgtgcg gggtcgagcg ccaggccggg gccgatcagc cattcgttgt cggcatgctg
      781 gccgccatac caggttggat agacgatcgc gttgtcgcgc gcggcgttca gggtgccatg
      841 cgtcttgtag gcaagcctgg cgccacgtag tgttgcgccg gattgcaaca caacattgcc
      901 catctcggca atttcgaacg tatctgaatt tttcgtcatg ggttaaacat ttgcggaaac
      961 ggtaaacaaa aaaagcccgt caaccagggt tggcctggtc ggcgggcttc attggccggg
     1021 gcctgcatgg acgcctttgt ccgtgcaggc aagtgatcaa ggagttgctg ctgtgtttca
     1081 gcgcttgctg gatgcatccg gcaggcaaca cgaggcgata ttgcatatgt cgtgcctgct
     1141 gacgcgacac gcagttaatc atgcattaaa gcaagtgctg gcttggcgca gatggccgcg
     1201 gcgcatctgt ttcgtgataa cgaagccgtc gatgtcgtgc accacgtgca ggcaaacacg
     1261 cactgtgctc ttgcacgcta tatgaaaatg caccgtgagc gccgctaacg cttgatgagc
     1321 cgctaccgcc aaatgctcaa tggcttgcgc gatacggttg tatggctcgt tctgcctctt
     1381 ctggcgtgca gattctttga acgcggcgtt catcgtcttg tcgcggaaga gccagcggcc
     1441 cgtggtcggg ctgctgctgt cgctgccggc atggcgcgcc attgccgacc ttggctatat
     1501 cggcgacacg gcgggcgctg atccgatctc tcggggagac ttgcacaata cctggtatct
     1561 cggcagtgca acgtcaggat tgcgcctgcg cgacgccatt gcgccgaggt tagaagctca
     1621 gggtttcgtg tgccttgacg atgtcgccgg ccaccgtctc gatctgttcg cgtttgagca
     1681 tggcttttga gcgcagcagt tgatacgcat cttcttcaga catgccgcgc gagtccatta
     1741 cgattcgcgt ggcctgctgg atcacccgaa ccttcgcctg cttctgctcg agccgttcga
     1801 tgtatcgctc acgtgcgcgg gatgccttga attgcgtgag tgcgacggcg acggcggtca
     1861 ataacccaaa cgagcgaacc ggcgccggga tcgtagcgaa cgcattcaac cgcagcaccg
     1921 cttcgatggt gatgggattt tcgtaggtca cgaccggaat caccggtggc gacgtgcttg
     1981 ttccgagcca cggataatcg atcgacagcg cctcaggacg tatcgccatg acaacgaggc
     2041 cggtgtccga aggcagcctg tcgaggcgcg gccagaatac ctgcacgtcg cagccgatgc
     2101 gctgaagctg cgcgagcaac tcttcgccat cctgatcatg cggatgaata acgacgacct
     2161 ttagcgaacg caagtcacgc aggatcgccg gcgtctgcgc ggtccgcttc atcgagtcgc
     2221 tccggcgcgt gcgagttcgg gatgggcgtg cgcggtccac gctggcgtgc tgtgaaagac
     2281 gacataggga tccgccttca cgcgttcagg cgcgcgtccg attatatcga aacggcaatg
     2341 cgcatcgacc cggccgatca acggatgcag gtatgtgtgg ttattgtgct cgtcgatacg
     2401 cacgcggcct tgcggcgcgt cgaactcgag tccgggcaac acgcgcagca acagcgtcgg
     2461 gtcgtcggaa ccgacgcgac gaatcgcatc cgcgagcaga tgcatctgga aataggacgc
     2521 ttcccagcac atgttggtcg gctgatcttc gccgaagcgg gcctgatggc gggcgacggc
     2581 gtcgtgattc gcggctgtat ccacactctg gaaataggtg gccgacgtga tgtgtccttc
     2641 tgcgaggtcg tttcccattg ccgcgatttc ggcttcgctg gtcatgtgac tggcgatagg
     2701 cgtgcggtac ggatcgagcc cgacctgcgc aaacgcacgg tacagatgcg gaataccttc
     2761 gccgaccacc gtggagaaaa tgaagtcggg cgcaagttgc ttgatccgct ttgcgacatc
     2821 cagataactc tcccacggcg catcgagcga cagataggtt tcgtccacct tttctccgcc
     2881 gcgtt
//
LOCUS       DATASH010000762         1929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_718495,
            whole genome shotgun sequence.
ACCESSION   DATASH010000762 DATASH010000000
VERSION     DATASH010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718495"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..828)
                     /locus_tag="VHV99_20360"
     CDS             complement(join(11..480,480..828))
                     /locus_tag="VHV99_20360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611267.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEX3637793.1"
                     /translation="MQAPLIDDELWALIEPLLPLARPRRKCDPGRPRISDRAALNGIL
                     FVLTTGLRWNHLPTRLGFGSGATCWRRLHDWQKAGVWDRLHNLLLGKLREAGQLDLSY
                     AAVDSSSIRAVGAGGKTGPNPTDRARPGSKHHILVDANGVPVSAILTGANRNDVTQLL
                     PLVDAIAPIRGVRGRPLRKPKIIYADRGYDSETHRRRLRERGIRPIIARRRTEHGSGL
                     GKFRWVVERTHSWLHNFRRLRIRFDRRADIHEAFLTFGCSLVCWNILRRAQQDF"
     gene            complement(984..1844)
                     /locus_tag="VHV99_20365"
     CDS             complement(984..1844)
                     /locus_tag="VHV99_20365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX3637794.1"
                     /translation="MNTLSLAHTVASPTPAASRTVGDLLREWRQRRRMSQLLLATEAD
                     ISTRHLSFVESGRALPSREMVMHLAERLEVPLRARNALLVAAGYAPLFRERPLSDPQL
                     AAAREAVELVLKGHEPYPALAIDRHWTIIAANNALAPLLNGASPELLKPPVNALRLSL
                     HPEGIAASIVNWHAWREHVLARLQRQIDVSGDETLSALRDELAAYPAPPGAHAPDNDD
                     TAVNQIAVPLRLRTPIGVLSFFSTTTVFGTPVDVTLSELAIEAFFPADQPTAAALREF
                     AENQRTEAER"
BASE COUNT          356 a          585 c          629 g          359 t
ORIGIN      
        1 agaggccagt tcaaaaatcc tgctgtgcac gcctgaggat gttccagcag acgagagagc
       61 agccgaatgt caggaatgcc tcgtgaatgt cggctcgacg atcgaaacga atgcgaagac
      121 gacggaagtt atgcagccac gagtgagtgc gttcaacgac ccaacggaat ttgcccagac
      181 cgctgccgtg ctctgtgcgg cgcctggcaa tgatcggcct gattccacgt tcacgcaggc
      241 gccgccgatg tgtttcggaa tcgtagcccc ggtcggcata gatgatcttg ggcttgcgaa
      301 gcgggcgacc gcgcacgcca cgaatcggcg caatcgcgtc gacgagcggc agcagttggg
      361 tcacgtcgtt gcggttcgcg ccggtcagga tcgcgctgac gggcacaccg ttggcatcta
      421 cgaggatgtg gtgtttggaa ccaggtcgcg cgcgatcggt ggggttcggg cctgtttttc
      481 gcccgcccca acggctcgta ttgacgagga gtcgaccgcg gcgtacgaga ggtcgagttg
      541 gccagcttcg cgcaatttgc cgagcaacag attgtgcagc cgatcccata caccggcctt
      601 ctgccagtcg tgcagccgtc gccagcaggt tgcgcctgac ccgaagccca ggcgtgtcgg
      661 cagatggttc caccgcagac ctgtggtcag cacgaacagg atgccattga gcgccgcgcg
      721 atcggatatg cgcggacgcc ccggatcgca tttgcgccgc ggcctggcta acggtagtaa
      781 cggttcgatc agtgcccaca attcatcgtc aatgagtggc gcttgcattc cttgggttcc
      841 gttgttcagg tgctcaaggt taacacctcg ccgggaaagt aaacagcccc ctcaaggggt
      901 ttttgaactg gcctctaagg gcccacgtgc ccgagcttgc ccttgtcttc cgttccggcg
      961 ccgggcaaag gcgatggaag ggtttatcgc tcagcttcgg tgcgttggtt ttccgcaaac
     1021 tcgcgcaatg ccgccgcggt tggttggtcg gcgggaaaga acgcttcgat cgcaagctcc
     1081 gatagggtga cgtctaccgg cgtaccgaac acggtggtcg tgctgaaaaa cgacagcacg
     1141 ccgatcggtg tgcgcagccg cagcggcacg gcgatctgat tgacggcggt gtcgtcattg
     1201 tcaggcgcgt gcgcgccagg cggcgcggga tacgccgcca gttcgtcgcg cagcgcgctc
     1261 aatgtctcgt cgccactcac gtcgatttgc cgctggagcc gggccaacac gtgttcccgc
     1321 catgcatgcc aattgacgat cgatgccgcg atgccttccg gatgcagact caaacgcagc
     1381 gcgttgaccg gcggtttcag cagttcggga ctggcgccgt tcagcagcgg cgcgagcgcg
     1441 ttgttcgccg cgatgatggt ccagtgccgg tcgatcgcga gcgccgggta gggctcgtgt
     1501 cctttgagca ccagctcgac ggcctcgcgc gcggcggcta attgcggatc ggacagcgga
     1561 cgctcgcgga acagcggcgc ataacccgcc gcgaccagca gcgcattgcg cgcgcgcagc
     1621 ggcacttcga gtcgctcggc aagatgcatg accatttccc ggctgggcaa cgcgcggccc
     1681 gattcgacga agctcaaatg ccgggtcgaa atgtcggcct cagtggcgag cagcaactgg
     1741 ctcattcttc gccgctgccg ccattcacgc agcaagtcgc cgaccgttcg actggccgcc
     1801 ggcgtgggcg aagcgaccgt gtgcgcaaga gagagtgtgt tcatgcatcc gatgatagcc
     1861 aaatcgggtg gacgatccat tacctggcgg gtaatggaaa accccggcga ctcctacgaa
     1921 tacccggaa
//
LOCUS       DATASH010000763         5106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5863025,
            whole genome shotgun sequence.
ACCESSION   DATASH010000763 DATASH010000000
VERSION     DATASH010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5106
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5863025"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..924)
                     /locus_tag="VHV99_20370"
     CDS             complement(163..924)
                     /locus_tag="VHV99_20370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX3637795.1"
                     /translation="MELNDALRIPLAPSAVWDALQDLALLRASLDNCESFSRLSHGEY
                     ALTLTVPLGPLRARYEARAHVAGQDSGPADAQRRTINFKARAEGIGALRGQIEVRLRA
                     EDGSHGIGRERGTRIDYTIWATSSGPLAELPTRQLENALHQLADDFFDEFGAVVRAKY
                     GQGPNRARGAAARRQYVFLRPITLGGVARRTRTDHGNAMSGRAASASHGAAHGVSHGV
                     SHREPGAHAVPHWAWAAMIFLVALLLYVARWISEH"
     gene            complement(957..1349)
                     /locus_tag="VHV99_20375"
     CDS             complement(957..1349)
                     /locus_tag="VHV99_20375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEX3637796.1"
                     /translation="MSDALTPHGSPGASTGGHTIGISGNNHRVRVIHGGVTMADTQAG
                     LTLTETGLADVFYFPRGDVNMARLERSTHTSHCPFKGEASYFHLRTEDGLIENAVWSY
                     ETPLEAVAQIKGYLAFYASRVDRIDQTS"
     gene            complement(1524..2018)
                     /locus_tag="VHV99_20380"
     CDS             complement(1524..2018)
                     /locus_tag="VHV99_20380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX3637797.1"
                     /translation="MKTQRNQRRIVAAAVCAALTLGLAGGAYAQASDVTGGTTTDESS
                     AGNANGGGLPQIQQQGDVSFVSGGVGLDESKALQQAQSQWPLSLRFTGPGSDFLADVH
                     VRVVDAHGGEVLSTTSRGPYMLVRLRPGRYTVHAQYKDRDQIKPVTIPAKGTAKAAFY
                     WNTQ"
     gene            complement(2380..3897)
                     /locus_tag="VHV99_20385"
     CDS             complement(2380..3897)
                     /locus_tag="VHV99_20385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEX3637798.1"
                     /translation="MNAKTLSRSAVAVAVAVALSAGYVAGHRDVPAPQVIAPAQAAMM
                     PAEAAAKTGIPDFSGLVETYGPAVVNISAKHVVKQAALRGNGGNGGNQLPIDPSDPFY
                     QFYKHFFGGVPGMQGGDGSDGGDAPDQPSASLGSGFIVSSDGYILTNAHVVDGANVVT
                     VKLTDKREFKAKVVGADKQSDIAVLKIDASSLPTVKIGDPSQSKVGQWVVAIGSPYGF
                     DNTVTSGIISAKSRSLPNENYTPFIQTDVPVNPGNSGGPLFNLQGEVIGINSMIYSRT
                     GGFQGLSFAIPINEAIKVKDDIVKTGHVSRGRLGVAVQGMNQTLANSFGMQKPQGALV
                     SSVDPGGPAAKGGLQPGDVILSVNGQPVGDSSDLPAQIAGLAPGSSATVQVWRDKATK
                     DLKVTIGSLSDVKVASDKADQPAQLQGRLGVAVRPLTPEEKSGASVSHGLLVQQSGGA
                     AENAGIQPGDVILAVNGRPVTTVDQLKQMIAAAGNSIALLIQRDNAQIFVPVDLG"
     gene            complement(4066..>5106)
                     /locus_tag="VHV99_20390"
     CDS             complement(4066..>5106)
                     /locus_tag="VHV99_20390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3637799.1"
                     /translation="APLAPRAELGFSTERTERGDWRVYGAIVGDNVVQLAQPVSVRNR
                     LAANVALRTLWPLIVLLPLLGVAVWIIVGRGLRPLRRVTSALDARHPEALDPLPDQRL
                     PLEVQPLVRALNGLLERLATALDIQKAFVADAAHELRTPLAAVQIQSQLVARAKDDTA
                     RSEALADLQAGVTRATRLAEQLLALARSEPDGLAATNAIDLRALLQDCVVAYAPLAQN
                     RSVDLGIEASEAATVIGDADALRVMFNNLVDNATKYTPRGGRVDVGLDVEEGHPVVRI
                     ADSGPGIEPAERDRVFDRFYRAGAGANRARTDVAGSGLGLAIVRRIATQHHAAVRLDE
                     SPTGGLQVEVRF"
BASE COUNT          896 a         1699 c         1577 g          934 t
ORIGIN      
        1 tggctgtggc tgtggctgtg gctgtggctg tggctgtggc tgtggctgtg gctgtggctg
       61 tggctgtggc tgtggctttg gctgtgactg tggccctaac gttacagttg ccgcaaacat
      121 cgccgtcacc aactcaacaa agccaaacga accgaaccca cttcagtgtt cgctaatcca
      181 tcgcgctaca tacaacagca gcgccaccag aaaaatcatc gccgcccacg cccagtgcgg
      241 aaccgcgtgc gcaccgggct cgcgatgcga gacgccatgc gacacaccat gggccgcccc
      301 atgcgacgcg ctcgccgccc gcccgctcat cgcgttgcca tgatccgtgc gagtccgccg
      361 agccacaccg ccgagcgtga tcggccgcag aaacacatac tgacggcgcg ccgccgcccc
      421 ccgcgcccgg ttcggccctt gcccatactt ggcacgaacg accgcaccaa actcgtcgaa
      481 aaaatcatcg gccagctggt gcagcgcgtt ttcgagctgg cgcgtgggca gttcggcgag
      541 cggccccgac gaggtcgccc agatcgtgta atcgatccgc gtgccccgct ctctgccgat
      601 gccatgcgag ccgtcctccg cgcgcaatct cacctcgatc tgaccgcgta acgcgccgat
      661 gccttcggct cgcgccttga agttgatggt gcggcgctgc gcgtccgccg gtccggaatc
      721 ctgaccggct acgtgagccc gcgcttcgta acgcgcgcgc aacggcccga gcggcacggt
      781 cagggtgagc gcatattcgc cgtggctgag ccggctgaac gactcgcagt tgtcaaggct
      841 ggcacgcaac aaggcgaggt cctgcaacgc gtcccaaacc gcggacggcg caagcggaat
      901 ccgtaacgcg tcgttcaatt ccatgccagc ctccccgatg aacgtgcgac aaccggtcag
      961 gatgtctgat cgatacggtc gacgcgcgat gcgtaaaacg cgagatatcc ttttatttgt
     1021 gccactgcct cgagcggcgt ctcatagctc cacacggcgt tttcgatcag accatcttca
     1081 gtgcgcagat ggaagtatga cgcctcgccc ttgaacgggc agtgcgaagt atgcgttgag
     1141 cgttcgaggc gcgccatgtt cacgtcgcca cgcgggaaat agaaaacatc cgcgagaccg
     1201 gtttccgtga gtgtcaggcc cgcttgggtg tcggccatgg tcacgccgcc atggatcaca
     1261 cgcacccggt gattgttgcc ggatatgccg atggtatgcc cgcccgtgct cgcgccgggg
     1321 cttccgtgag gtgtcaaggc atcgctcatg gctcggctcc gtgatggtgt ccaactcgat
     1381 gtgcaactgc tgtgcgaaag tacaaacaat aaagccacgg gttcgcgccc gtggcttcat
     1441 tatcggccaa acttccgccg cttgcgcggc cttcctgaat gcagaagagg gatctccccg
     1501 acgccgctca accggcgggc cgcttattgc gtattccaat agaaggcggc cttggcggtg
     1561 cccttggccg ggatcgtcac cggtttgatc tggtcgcgat ccttgtattg cgcatgcacg
     1621 gtgtaacggc ccggccgcag cctgaccagc atgtacggcc cgcgcgacgt ggtgctcaac
     1681 acttcgccgc cgtgcgcgtc gaccacccgc acgtggacgt cggcaagaaa atcggagccg
     1741 ggtccggtga agcgcagcga cagtggccac tggctttgcg cctgctgcag tgctttcgat
     1801 tcgtcaaggc cgacgccgcc cgacacgaac gagacgtcgc cttgctgctg gatttgcggc
     1861 aggccgccac cattggcgtt gcccgcgctg ctttcgtcgg tggtggtgcc gcccgttacg
     1921 tcactcgcct gtgcataagc gccgcccgcc agaccgagcg tgagcgctgc gcataccgcg
     1981 gcggctacga tccttcgctg attgcgttgg gttttcatcg gttcgctcct tattaaggtc
     2041 cttgtcgtcc ggtatccgtc gatggacccg gactaaggca gacgcaacct gcgtgccaac
     2101 cagcaaacgt gtcgtttgga aaccgctgct gcaccgcgcc acgcggcgcg gagcgccctt
     2161 cttcatggct tgggaagggg tgaaaaaaat gccggtccgt cggcagtaag tgcaaggacc
     2221 ggcaagcgtt gcttcaattt ccgggcaaga tcggcccgcg cgtcagtcgg agtggccgcc
     2281 aaacgatcag ccgtgtcagt caacgtgcca gccaaacgat caaccacgcg atcattcaag
     2341 cccgccaaca cgctgaccga ctcaccgaac cgccgatcct cagccgagat ccaccggcac
     2401 gaagatctgc gcgttatcgc gctggatcag cagggcgatg ctgttgcccg cggcggcaat
     2461 catctgcttc aactgatcga cggtcgtgac cggccgccca ttcaccgcca gaatcacgtc
     2521 gcccggctgg atgccggcgt tttcagccgc gccgcccgat tgctgcacga gcagaccgtg
     2581 agagaccgac gcgccgctct tttcttcggg cgtcagcggc cgcaccgcca cgccgagacg
     2641 gccttgcaac tgggccggct gatcggcctt gtccgaagcc acctttacat ccgacaacga
     2701 accgatcgtc accttcagat ccttggtggc cttgtcgcgc catacctgca cggtggcgga
     2761 gctgcccggc gccagacctg cgatctgcgc cggcagatcc gacgagtcgc cgaccggttg
     2821 accattcacc gacagaatca cgtcgcccgg ttgcaaaccg cccttggcgg ccgggccgcc
     2881 cggatcgacc gagctgacga gcgcgccttg cggcttttgc atgccgaacg aattggccag
     2941 cgtctggttc atgccctgca ccgcgacgcc gagacgtccg cggctcacgt ggccggtctt
     3001 gacgatgtcg tccttgacct tgatcgcttc gttgatcgga atcgcgaacg aaaggccctg
     3061 gaagccgcct gtgcgcgaat agatcatcga gttgatgcca atcacctcgc cctgcaagtt
     3121 gaacagcggt ccacccgagt tacccggatt caccggcacg tcggtctgga tgaacggcgt
     3181 gtagttttcg ttcggcagcg aacgcgactt cgcgctgatg atgcccgagg tcaccgtgtt
     3241 gtcgaaaccg taaggcgagc cgatcgcgac cacccactgg ccgaccttgc tctgactcgg
     3301 atcgccgatc ttcacggtcg gcaaactgct tgcgtcgatc ttcagcacgg caatgtcgga
     3361 ctgcttgtcg gcaccgacca ctttcgcctt gaattcacgc ttgtcggtca gcttcacggt
     3421 gacgacgttc gcgccgtcca ccacatgggc gttggtcagg atgtagccgt cactgctgac
     3481 gatgaacccc gagcccaggc tcgcgctcgg ttgatcgggc gcgtcgccgc cgtcgctgcc
     3541 gtcgccgcct tgcatgcccg gcacaccgcc gaagaagtgc ttgtagaact gatagaacgg
     3601 atcgctcgga tcgatcggca actgattgcc gccattaccg ccgttgccgc gcaatgccgc
     3661 ctgtttgacc acatgcttcg cactgatgtt gacgacggcc gggccgtaag tttcgaccag
     3721 acccgagaaa tcggggatgc cggtcttcgc ggctgcttca gcaggcatca tcgcggcctg
     3781 cgccggcgcg atcacctgcg gcgcgggcac gtcgcgatgc cccgccacat agccggcgga
     3841 aagcgccacg gcgacagcca ctgcaacagc gctgcgggac aaggttttcg cgttcatcgt
     3901 gtactcctga ctgggagaaa gaggctttgg aatgagatga agcgtacgtg gcatcgctta
     3961 aaagagtctt aagcagcgtc tgatcgcttc agagcagttt tttaggcgcg tttaagcctc
     4021 gcttaagcct ggttcgaacc actttacgaa cagaaagtct cacactcaga agcggacttc
     4081 gacctgcaac ccacccgtcg gcgattcgtc gagccgcact gccgcatggt gttgggtagc
     4141 gatgcgccgc acgatcgcga ggccgaggcc gctgccggcc acatcggtgc gcgcgcggtt
     4201 cgcgcccgcg ccggcccggt agaagcgatc gaatacgcgg tcgcgttcgg ccggctcgat
     4261 gcctggcccg ctgtcggcga tccggaccac cgggtgccct tcttcgacat ccagaccaac
     4321 gtcgacgcgc ccgcctcgcg gcgtgtattt ggttgcgtta tcaacgaggt tgttgaacat
     4381 gacccgcagc gcgtcggcgt cgccgatgac cgtggcggct tcgctcgcct cgataccgag
     4441 atcgacgctg cgattctgcg caagcggcgc gtacgccacc acgcagtcct gcagcagcgc
     4501 acgcagatcg atcgcgttgg tcgcggcgag gccgtccggt tccgagcgtg cgagcgccag
     4561 caattgctcg gcaagccgcg tggcgcgcgt gacgccggct tgcaggtcgg ccagcgcttc
     4621 gctgcgcgcc gtatcgtctt tcgcgcgggc aaccagttgc gattggatct gcaccgcggc
     4681 gagcggcgtg cgcaattcgt gcgcggcgtc ggcaacgaat gctttctgaa tgtcgagcgc
     4741 ggtggcgagg cgttcgagca ggccgttcaa cgcgcgcacc agcggctgca cttcgagcgg
     4801 caggcgctga tcgggcaagg gatcgagcgc ctcggggtga cgcgcgtcga gcgcgctggt
     4861 cacgcgccgc aacggccgca gccctcgtcc gacaatgatc cagaccgcga cgcccaataa
     4921 cggcaggagc acgatcagcg gccacaaggt ccgcaacgcg acattcgccg cgagccggtt
     4981 gcgcaccgaa accggctgcg ccagctgcac gacgttatcg ccgacaatcg cgccatacac
     5041 acgccaatcg ccgcgctccg tgcgctcggt ggaaaagccg agttcggcgc gtggcgcgag
     5101 cggcgc
//
LOCUS       DATASH010000764         1904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1511754,
            whole genome shotgun sequence.
ACCESSION   DATASH010000764 DATASH010000000
VERSION     DATASH010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1511754"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="VHV99_20395"
     CDS             complement(<1..572)
                     /locus_tag="VHV99_20395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEX3637800.1"
                     /translation="MRIKLINPNTTQRMTDAMGRCAREVAAPGTEVIAVNPTMGPPSI
                     EGYYDEALATPGLLAEVIAGEREGCDAYVIACFGDPGLYAARELARGPVIGIAEAAMH
                     AASVLAPGFSVVTTLARTCGMAWHLAERYGMKRFCCNVRAIDVAVLDLDKFGSAARCV
                     ILDECRRALEEDGSDAIVLGCAGMAELCAE"
     gene            complement(612..>1904)
                     /locus_tag="VHV99_20400"
     CDS             complement(612..>1904)
                     /locus_tag="VHV99_20400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family nucleobase:cation symporter-1"
                     /protein_id="HEX3637801.1"
                     /translation="VGGYVFAGSLFALGLTSWQVLIALLVGIAIVNVLCNLIAKPSQA
                     NGVPYPVACRATFGVLGANIPAVIRGLIAVAWYGIQTYLASSALVIVVLKFVPQLLPY
                     ADVHHHGFMGLSTLGWTGFMLLWVLQALVFWHGMETIKKFIDFAGPAVYVVMFILAGY
                     MVYRAGWRNIGINLGGVKYHGMEVVPVMITATSLVVSYFSGPMLNFGDFSRYGKSFRS
                     VQRGNFWGLPVNFLAFSLVTVITTAATLPVFGQLITDPVETVGRIDYPTAVILGALTF
                     TIATIGINIVANFVSPAFDFSNVAPRLISWRAGGMLAAVASIFITPWNLFNNPAVIHY
                     TLDVLGSFIGPLYGVLIVDYFLVKRQKIVLDDLYSVSPSGSYWYRNGVNYRAVAALLP
                     AALIAVICVMVPTLDGLANFSWFIGAGLAAVFYRVLAR"
BASE COUNT          444 a          614 c          543 g          303 t
ORIGIN      
        1 atctccgcgc acaattcggc catgccggcg caaccgagca cgatcgcgtc cgacccatct
       61 tcctccagcg cgcgccggca ttcgtcgagg atgacgcagc gcgccgccga gccgaacttg
      121 tccagatcaa gcacggcgac gtcaatggcc cgcacgttac agcagaaacg cttcatgccg
      181 tagcgctcgg cgaggtgcca cgccatgccg caggtgcgcg ccaaggtggt cactaccgaa
      241 aaccccggcg ccagcacgct tgccgcatgc atcgcggctt cggcgatacc gatcaccgga
      301 ccgcgcgcca gttcacgtgc cgcgtacaga ccggggtcgc cgaaacaggc gatcacgtaa
      361 gcatcgcagc cctcacgttc gccggcaatg acctctgcca gcaagccggg cgtcgccagt
      421 gcttcgtcgt aatagccttc aatcgacggc ggccccatcg tcggattcac cgcaatcact
      481 tcggttccgg gcgcggcgac ttcgcgcgca cagcggccca tggcatccgt catacgttgt
      541 gtcgtatttg gattaatcag tttgatgcgc atatccactc ctgttgcgac tcaaatgacc
      601 cagcgtgcaa attagcgtgc aagtacacga tagaacaccg cggctagccc tgcgccgata
      661 aaccacgaga agttcgcgag tccatcgagc gtcggcacca tcacgcagat cacggcgatc
      721 aacgcggccg gcagcaatgc agccaccgcg cggtaattga cgccgttacg gtaccagtac
      781 gagccgctcg gggaaaccga atagagatca tcgagcacga tcttttgacg cttgacgagg
      841 aagtaatcaa cgatcaggac gccatacaaa ggaccgatga aactgccgag cacgtcgagc
      901 gtgtagtgaa tcaccgccgg attgttgaac aggttccacg gcgtgatgaa gatcgacgcc
      961 accgccgcga gcatgccgcc cgcgcgccag ctgatcagac gcggcgcgac attcgagaag
     1021 tcgaaggccg gcgacacgaa gttggcgacg atgttgatgc cgatcgtcgc aatcgtgaag
     1081 gtcaaggcac cgagaatcac cgcggtcgga tagtcgatac ggcccaccgt ttcgaccggg
     1141 tccgtgatca gttggccaaa taccggcagc gtcgccgcgg tcgtgatgac cgtcaccagc
     1201 gagaaggcga ggaagttgac cggcaagccc cagaagttac cgcgctgcac gctgcgaaag
     1261 ctcttgccgt agcgggaaaa gtcgccgaaa ttcagcatcg gtccggagaa gtacgagacc
     1321 accagcgagg tcgccgtgat catcaccggc accacttcca tgccgtgata tttgacgccg
     1381 ccgagattga tgccgatgtt tcgccatcct gcgcgataca ccatgtagcc cgcaagaatg
     1441 aacatcacca cgtagacggc cggaccggcg aagtcgatga actttttgat ggtctccatg
     1501 ccgtgccaga acacgagcgc ctgcaagacc cacagcaaca tgaagcctgt ccagcccaag
     1561 gttgagagac ccatgaagcc atggtgatgc acatccgcat agggcaacag ttgcggaacg
     1621 aacttcagca ccacgatcac cagcgcgctc gacgccagat aggtttgaat gccgtaccac
     1681 gcaaccgcga tcaaaccgcg aatcacggcg gggatattcg cgccgagcac gccgaacgtt
     1741 gcgcggcaag ccaccggata aggcacgccg ttcgcctgac tcggcttcgc aatcaggttg
     1801 cagagcacgt tgacaatcgc aatgccgacc agcagcgcga tcaatacctg ccagctagtc
     1861 agcccgagcg cgaacagact gcccgcgaac acatagccgc cgac
//
LOCUS       DATASH010000765        14801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4480081,
            whole genome shotgun sequence.
ACCESSION   DATASH010000765 DATASH010000000
VERSION     DATASH010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 14801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14801
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4480081"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VHV99_20405"
     CDS             <1..201
                     /locus_tag="VHV99_20405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637802.1"
                     /translation="IEVAAVESRDFRLKHAIPNRYWFFAKGYWTVCSMIVHDAFNTSN
                     TVAGIVGVARKSNRRAYDWRQA"
     gene            222..1139
                     /locus_tag="VHV99_20410"
     CDS             222..1139
                     /locus_tag="VHV99_20410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0018576 - catechol 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0009712 - catechol-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HEX3637803.1"
                     /translation="MTQTVLAELARAPNARFREIMSAAVQHLHDFARDAKLTEAEFHQ
                     ACAFIAKLGQLSNASHNEVVLAAGSVGLSSLVCLLNNGDNGQTETTANLMGPFWRMDS
                     PATPNGGSIVRSDTPGVPIFVDAWVHDAGGKPVEGAEVDVWHTSAEGFYENQDPEQAD
                     MNLRGKLTTDSEGHISFRSVKPAGYPIPLSGPVGELLRAQGRHNMRPAHIHFMIYKPG
                     FKTQFSQVYSSDDPNLETDVQFGVTQALVGQYVLHENESAPSGDIQGPWYSLAHHFRI
                     EAGEAKLPKPPITGKAEGPRPVWSVLERK"
     gene            1139..1894
                     /locus_tag="VHV99_20415"
     CDS             1139..1894
                     /locus_tag="VHV99_20415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX3637804.1"
                     /translation="MSDAQLEQLLQRGREQLAPAGVLRAAINLGNTVLAQRSAGNGEL
                     GGVSVDLARELATRLGVEVKFVTYEAAGKVFEALASHAWDVAFLAIDPARGEQIAFTP
                     PYVLIEGAYMVREASPLQRSEQVDRNAVRVSVGAGSAYDLYLTRTLKEARIERHASAA
                     EAFEAFIAQGFDAAAGVRQVVSRYASTHEGLRVLEDSFMTIRQAMGVPKDRPEAAAAL
                     AVFIEEMKAQGRIADSLARSGQTAAAVAPPAAI"
     gene            1936..2148
                     /locus_tag="VHV99_20420"
     CDS             1936..2148
                     /locus_tag="VHV99_20420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HEX3637805.1"
                     /translation="MPIIHISLVEGRDDAAVKACVKAVARTVHETLGAPLNTIRVYAT
                     VTPATHWAVGDQTKDELGAAAQENGK"
     gene            2145..2963
                     /locus_tag="VHV99_20425"
     CDS             2145..2963
                     /locus_tag="VHV99_20425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX3637806.1"
                     /translation="MTPQLIQKAANALVEAERSGKYIAPLRETYEQLTIEDAYAIQRA
                     NTDRRVAEGRRIVGCKIGLTSAAVQKQLGVDQPDFGMLFDDMGYGDGEPIPASILTQP
                     KIEAEVAFVIGRDLRMSNPAQIDVLNAIDYALPALEIVGSRIANWNIRITDTIADNAS
                     SSAYVLGNSPRKLSEFDLRLCGMVMERRGEPVSVGAGAACLGNPINAVLWLARKMAAL
                     GTPLLAGDLVLSGALGPMVPVTPGDIFETRINGLGSVRAVFEAESTKIASEAAQ"
     gene            2960..3727
                     /locus_tag="VHV99_20430"
     CDS             2960..3727
                     /locus_tag="VHV99_20430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX3637807.1"
                     /translation="MSSITEYATLLDDAARHATEVAQFDSEGRLSLDEAYAIQSASIN
                     RRVERGERRVGVKMGFTSRAKMVQMGLSDVIWGRLTAGMQIEEGTAIDFSRFVHPRVE
                     PEIAFVLKHSLEGDVTGPQALAAVEAIAPALEIIDSRYKDFKFTLPEVIADNASSSGF
                     VLGAWRDPKLDFANLGLTLNIDGRVVQVGSTAALLGHPLRSLVAAARLSAQAGEPLQA
                     GWIVMAGGATPAEWIKPGQYVSVDMEQLGSAGFHILG"
     gene            3883..4695
                     /locus_tag="VHV99_20435"
     CDS             3883..4695
                     /locus_tag="VHV99_20435"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX3637808.1"
                     /translation="MTRLYSLVPLTAIQLTPPELVAAASRTGYDAVSLRLSPFRAGEA
                     QHPMFDDSPMLLETEARLRDTGLEVLDIEVMLLTPERDVQDFKRVFETATRLGARNAL
                     TLIDIADRPLAVEKFGQLCELAAHFGISCALEFAAWLGVGSIQTANAIVSEAGQTNGA
                     MLLDPFHLIRSGGKVSDIAAVEPARIRYAQFCDASGKAPDTTAGISEEARYDRLLPGE
                     GGLPLREFVAALPAGIPFGLEVPNRRLAESLELDERLRATLAAAKRVAGDQS"
     gene            4705..4905
                     /locus_tag="VHV99_20440"
                     /pseudo
     CDS             4705..4905
                     /locus_tag="VHV99_20440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566059.1"
                     /note="involved in the transport of galactonate;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            4933..5766
                     /locus_tag="VHV99_20445"
     CDS             4933..5766
                     /locus_tag="VHV99_20445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX3637809.1"
                     /translation="MISGKTTLIAHLGYPTEGFKAPLIYNPWFDKNGIDAVVVPMGVL
                     ADDYKALLPMLFRLTNIRGALVTMPHKVTTTGLVDEMTPTARIAGACNAILKREDGTL
                     LGDQFDGAGFVRGVQRKGRELTGARVLVLGSGGVGSAIAASLAGAGVADMALFDTATQ
                     SVEALAGRLREHYPALRVSTGSKDPAGFDLIVNATPLGMKDGDPIPFDVERIAPTTFV
                     GEVVMKAEYTPLLRAAKDKGCPVQVGTDMLYEMIPAYLEFFGFGTATPEELRAVSQIK
                     Q"
     gene            5784..6239
                     /gene="aroQ"
                     /locus_tag="VHV99_20450"
     CDS             5784..6239
                     /gene="aroQ"
                     /locus_tag="VHV99_20450"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011004647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEX3637810.1"
                     /translation="MKKVLVLNGPNLNLLGQREPAQYGYDTLATVEQLCKEAGERLGV
                     EVECRQSNHEGFLIDWIHEAGREIAAGNMLGVVMNPGAFTHTSVALHDAIKGANVTLI
                     ELHISNVHARESFRHHSYISPAARGIVVGLGVKGYGIAIDALVQISEQK"
     gene            6375..6560
                     /locus_tag="VHV99_20455"
                     /pseudo
     CDS             6375..6560
                     /locus_tag="VHV99_20455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006752204.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
     gene            6502..7512
                     /locus_tag="VHV99_20460"
     CDS             6502..7512
                     /locus_tag="VHV99_20460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007584218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEX3637811.1"
                     /translation="MSAIDRLARIFDQLHPRVAEILRNFSAASVSLKAISGRRKGGMQ
                     HSLAHLRRLLGRDDAPGRRRPRRERANEVIVRITLDAQHAAALRHALIRDCGGQSWTI
                     RLAPLRGTDQMQLALYLPKAAVNDAMRRVSIIAPGAAVDHMLEIPDVPSDAWRDLTRD
                     SVPERVDSRRRLPGAARSSHAIADLLRREHVLLDLDAGDREALFVEIGRVVERYHGLS
                     AATVTAGLLAREALGSTGLGQGVAVPHGQISGLDQELVLYVRPALPILFDAPDGYPVS
                     DAIVLLVPEWANTMHLHLLADAAQHFCDHHFRERLRLCRDANSVLQLFKDYETQGEAM
                     HR"
     gene            7595..8011
                     /locus_tag="VHV99_20465"
     CDS             7595..8011
                     /locus_tag="VHV99_20465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX3637812.1"
                     /translation="MFRMLIPVLDHGGAAEAARFAASMFLERCLTEVEILEILEPVDQ
                     GRAAAFHTRSSLVRLEKRAMLKALVDARAILEEAGVPYKWKRIFGHRTKAIAAYAATT
                     QPDVMVIDASHMGIFRRLATLAALSMRTSTPVTMVH"
     gene            8172..8684
                     /locus_tag="VHV99_20470"
     CDS             8172..8684
                     /locus_tag="VHV99_20470"
                     /inference="COORDINATES: protein motif:HMM:NF019544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3637813.1"
                     /translation="MKPTAIVLHGPTSAGKSSLARALQDSSEIPVFHISLDAFVEMSR
                     RRDMRSDEELNQALRLHHLNLQSVLKRVAASHFEIVLDLVLRDTSALDECISALAPRC
                     TYVIGVSCPLNVLEERERGRSDRGEGMARSQFGHPAYTMPYSMRLDTSTCTPEDGARR
                     IRSFINAQIE"
     gene            complement(8758..9576)
                     /locus_tag="VHV99_20475"
     CDS             complement(8758..9576)
                     /locus_tag="VHV99_20475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016346907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3637814.1"
                     /translation="MDGFGTDTRFAGSIPELYDRYLVPLIFEPYAADLANRVAVLKPS
                     RVLETAAGTGVVTRAMAYALRTHVDLVATDLNQPMLDRAAAIGTPRPVKWQQADATRL
                     PFEDASFDVVVCQFGVMFFPDKALAFCEARRVLRHGGVLLFNVWDRIEENEFADTVTA
                     ALAALFPTDPPLFMVRTPHGYFDPQVISRDLAEAGFSTAPRFETIVARSRAASAREPA
                     VAYCQGTPLRNEIEARSGASVDDATSASATAIAERFGAGPVDGKIQAHVVIAQR"
     gene            9867..10505
                     /locus_tag="VHV99_20480"
     CDS             9867..10505
                     /locus_tag="VHV99_20480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HEX3637815.1"
                     /translation="MEMMMATPTWKISGQYYETCSCDFVCPCLPGQMAVRPSKGSCTF
                     AMAFQIDRGQHEAVSLDGLGFIVLGLTPEAMAKGNWSVGLIVDERASTEQRDAITAIT
                     SGTAGGPMAALSPLVGSFVGVESAAIRFDRNGTKWSVTSPQFVDMAAEGAMGINPNAT
                     EPLHLDNTGHPAADRVALAHASRSHVRALGFEWDDVSGQNNGQYAPFSWQGA"
     gene            10615..11319
                     /locus_tag="VHV99_20485"
     CDS             10615..11319
                     /locus_tag="VHV99_20485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HEX3637816.1"
                     /translation="MYLFYLDHQMLSARQHDKMMMEMGMSMAAPWTTADVFFTFAMWV
                     VMMMGMMAGSAMPVLLLFAGAHARRGGGRAPFVVLMFASGYLAVWVGFSACATLVQWA
                     LHHAALLSPELGASSPQVGGAILCAAGLYQLTPFKRACLMHCRSPLGFLMTRWRDGPS
                     GALQMGMLHGAYCLGCCWALMCVLFVVGVMNLVWVAALALFVLLEKIGPAGAVLARAA
                     GAAMIAVGVLLFAGML"
     gene            11690..12745
                     /locus_tag="VHV99_20490"
     CDS             11690..12745
                     /locus_tag="VHV99_20490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011153948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HEX3637817.1"
                     /translation="MTELVKALPAATPGHPRRQIVWLYALLLIFNLAAWLAAFTAFRG
                     YPLLMGSCLLAYSFGLRHAVDADHIAAIDNVTRKLMQAGQRPLTAGLMFSLGHSTIVV
                     LATLGIAVTAMALQTRLSGFRGAGAMIGTLVSTFFLFAIALLNLIVLRSVLRAFRRVR
                     RGEPYVDEDLDMLLANRGFLARIFRPLFRLITRSWHMYPLGFLFGLGFDTATEVGLLG
                     ISAAGAAQGMSIWSILVFPVLFTAGMTLIDTTDSILMLGAYGWAFVKPVRKLYYNLTI
                     TAMSVLVALLVGSIEGLGLLADKLHLNGGFWQAVAVLNDNFGMLGYAIIGVFAAAWLL
                     SVAVYKWQRIGEIEIHS"
     gene            13082..14140
                     /locus_tag="VHV99_20495"
     CDS             13082..14140
                     /locus_tag="VHV99_20495"
                     /inference="COORDINATES: protein motif:HMM:NF013242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637818.1"
                     /translation="MAEADNVPYGRKTQHAPALTEVHIIWITAGLGCDGDSVSITAAT
                     QPSIEDVILGAIPGLPKVHLHNPVLAYENGDEFLKPFHQAARGELDNFVLVMEGSIPN
                     ERINREGYWAALGTDPDTGDPITIPQWLDRLAPRALAVVGAGTCATYGGIHAMQGNPT
                     GCMGLADYLGWDWKSKAGLPIVNVPGCPVQPDNFMETLLYLLYQLAGLAPMIPLDDAL
                     RPKWLFTRTVHDGCDRAGSYEQAIFATEYGSPNCIVKLGCWGPVVQCNVPKRGWMAGI
                     GGCPNVGGICIGCTMPGFPDKFMPFMEEPPGAVLSSNLIKSYGPLIRSLRKLTRNTLN
                     DEPKWRHNRSELTTGYRR"
     gene            14194..>14801
                     /locus_tag="VHV99_20500"
     CDS             14194..>14801
                     /locus_tag="VHV99_20500"
                     /inference="COORDINATES: protein motif:HMM:NF012592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HEX3637819.1"
                     /translation="MAISTAPEATSSTQAAPGKLVEMNWDPITRIVGSLGIYTKIDFA
                     NRRVAECYSTSSIFRGYSIFMKGKDPRDAHFITSRICGICGDNHATCSVYAQNMAYGV
                     KPPPIAEWIVNLGEAAEYMFDHNIFQDNLVGVDFCEKRVRETNPGVWDKAQKTPAPHA
                     KDHGYRTIADIMRALNPFEGEFYREALVMSRLTREMFCLMEGR"
BASE COUNT         2892 a         4335 c         4641 g         2933 t
ORIGIN      
        1 attgaagtgg ccgcggtgga aagtcgagat ttccgtctga agcatgcgat accgaatcgg
       61 tattggtttt tcgcaaaagg gtattggacc gtatgttcga tgatcgtgca tgatgccttc
      121 aacacgtcaa acaccgtcgc cggcatcgtc ggggtcgctc gcaagtcaaa tagacgcgca
      181 tacgattgga gacaggcatg atcataagca accagagaga tgtgacacag actgtccttg
      241 cagagttggc gcgcgcgccg aacgcacgtt tccgcgaaat catgtccgcc gcagtgcagc
      301 atctgcatga tttcgctcgc gacgccaagc ttaccgaagc ggaattccat caagcgtgtg
      361 ccttcatcgc gaagctcggg caattgtcga atgcatcgca caacgaggtc gttctggcgg
      421 cgggttccgt cgggctatct tcgctggtgt gtctgctgaa taacggcgac aacggccaga
      481 ccgaaaccac cgccaacctg atgggaccgt tctggcggat ggattcgccc gctacgccca
      541 acggcggttc gatcgtgcgt tcggacacgc ccggcgtgcc gatctttgtc gacgcatggg
      601 tgcacgacgc cgggggcaaa cctgtggaag gcgcggaagt cgatgtgtgg cacacgtcgg
      661 ccgaagggtt ttatgaaaac caggacccgg agcaggccga catgaatctg cgcggcaaac
      721 tcacgacgga tagtgaagga catatctcct ttcgcagcgt gaagccggcg ggatatccga
      781 ttccgttgtc gggcccggtc ggagaattgc tgcgcgcgca aggacgccac aatatgcgcc
      841 ccgctcacat ccacttcatg atctacaagc cgggtttcaa gacgcagttc tcgcaggtct
      901 attcgagcga cgatcccaat ctggaaaccg atgtgcaatt cggcgtaacc caggcgctgg
      961 tcggccagta tgtgttgcac gaaaacgaat ccgcaccgtc cggcgatatc caggggccgt
     1021 ggtattcgct ggcgcatcac ttcaggatcg aggcaggcga ggcgaaattg ccgaagccgc
     1081 cgatcaccgg caaagccgag gggccgcgtc cggtgtggtc ggtgctggag cgcaagtgat
     1141 gagcgacgca cagcttgagc agttacttca gcgcgggcgt gaacaactgg cgccggccgg
     1201 cgtactgcgt gccgcgatca atctcggcaa caccgtgctt gcacagcgct ctgccgggaa
     1261 cggcgaattg ggaggcgttt ccgttgacct tgcgcgtgag ctcgcaacaa ggctgggcgt
     1321 cgaggtgaaa ttcgtcacct atgaagcggc aggcaaggtt tttgaagcgc tcgccagtca
     1381 tgcatgggac gttgcgtttc tcgcgatcga tccggctcgt ggcgagcaga tcgctttcac
     1441 cccgccgtat gtgctgatag aaggcgcgta catggtgcgc gaggcatcgc cgttgcagcg
     1501 tagtgagcag gtggaccgca acgccgtacg cgtgagcgtg ggtgctggca gtgcatatga
     1561 cctgtatctg acgcgcacgc tcaaagaggc ccggatcgaa cgtcatgcct ctgcggctga
     1621 agcgttcgag gcgttcattg cgcaaggctt tgacgctgct gccggagtgc gtcaggtggt
     1681 gagccgctat gcctcgacgc atgagggctt acgcgtactc gaagacagct tcatgacgat
     1741 ccgtcaggcg atgggtgtgc cgaaggatcg ccccgaggca gccgcagcgc tggcggtttt
     1801 catcgaagaa atgaaagcgc aggggcgcat cgccgattcg ctcgcacgga gcgggcaaac
     1861 ggcggccgca gtggcgcctc ccgcggcaat ttaattcaac ccggcgtcac gtcacggtac
     1921 gcccacagga aaaccatgcc catcatccat atttccctcg tcgaaggacg tgacgacgcc
     1981 gccgtcaagg cgtgcgtcaa ggctgtggcc cgcacggtgc acgagacgct aggcgcgcca
     2041 ctcaacacca tccgcgtgta cgcgacggtc acgccggcca cgcattgggc cgtcggcgat
     2101 caaaccaagg acgaactcgg tgcagcggca caggagaacg gaaaatgaca ccgcaattga
     2161 ttcaaaaggc ggccaacgcg ttggtcgagg ccgagcgtag cggcaagtac attgcaccgt
     2221 tgcgcgaaac ctacgagcaa ctgacgatcg aagatgctta cgcgatccag cgtgccaaca
     2281 ccgatcggcg cgtggccgaa ggacggcgca ttgtcggctg caagatcgga ttgacctcgg
     2341 ccgccgtgca aaagcaactg ggcgtcgatc agccggactt tggcatgctg ttcgacgaca
     2401 tgggttacgg cgacggcgaa cccattcccg cgtcgattct cactcaaccg aagattgaag
     2461 ccgaagtcgc cttcgtgatc ggccgcgatc tgcggatgtc gaatcccgct cagatcgacg
     2521 tgctgaacgc tatcgactac gcgctgccgg cgctcgagat tgtcggcagc cggatcgcga
     2581 actggaatat ccgcatcacc gacacgattg cggacaacgc gtcgtcgtcg gcatatgtac
     2641 tcggcaacag cccgcgcaag ttgtccgaat tcgaccttcg gctgtgcggc atggtgatgg
     2701 agcggcgcgg tgagcccgta tccgtcggcg cgggcgctgc atgcctgggt aacccgatca
     2761 atgcggtcct ctggctggct cgcaagatgg ctgcgctcgg cacaccgctt ctggcgggcg
     2821 acctggtgct gtccggcgcg ctcggaccga tggtgcccgt cacgcccggc gacatcttcg
     2881 aaacccgcat caacggattg ggctcggtgc gtgccgtgtt cgaagccgaa tccacgaaaa
     2941 ttgcgagcga ggcagcccaa tgagctctat taccgaatac gcgacgctgc tcgacgatgc
     3001 agcgcgccat gcaaccgaag tcgcccagtt cgattctgaa ggccgcctgt cactggacga
     3061 ggcctacgcg atccagtccg cgtcgatcaa tcggcgtgtt gaacggggcg agcggcgcgt
     3121 cggcgtgaag atgggtttca cgagccgggc aaagatggtt cagatgggtc tctccgatgt
     3181 gatctggggg cgcctcaccg ccggtatgca gatcgaggaa ggcaccgcga tcgatttctc
     3241 gcgtttcgtg catccgcgcg tcgaaccgga aatcgccttc gtgttgaagc attcgctcga
     3301 aggcgacgtg accggaccac aagcgctcgc tgcggttgaa gcgattgccc ctgccctcga
     3361 aatcatcgac tcccgctaca aagacttcaa attcacgttg cccgaggtga tcgcggataa
     3421 cgcttcgtca agcggtttcg tgctcggcgc gtggcgcgat ccgaagctcg actttgccaa
     3481 tctgggcctg acgttgaaca tcgacggccg tgtcgtgcaa gttggatcga ccgcggcgct
     3541 gctgggtcat ccgctgcgct cgctggttgc cgccgcacgc ttgtccgcac aggcaggcga
     3601 gccgttgcag gccggctgga tcgtgatggc gggtggcgcg acgccggccg aatggatcaa
     3661 gcccgggcaa tacgtatcgg tcgatatgga acagctcggc agcgcgggtt ttcacatctt
     3721 ggggtaggcg atgccaattg ctatgatcaa gatccttgag gggccgcagt ccgagaagac
     3781 atgatcgtcg acgtcgcact cgatctgctg aagcgggttg cgcacgagca gacgcacgtc
     3841 gaacccacca tgagcgaagc aggctaacgc acggacgatg cgatgacacg gctctactcc
     3901 ctggtaccgc ttaccgcaat ccagctaacg ccgcctgaac tggtggcggc agcgagccgc
     3961 acgggctacg acgcggttag cctgcgcctg agcccgtttc gggcgggcga ggcgcagcat
     4021 ccgatgttcg acgattcgcc aatgttgctt gaaacggaag cacgcttgcg cgataccggt
     4081 ctcgaagtgc tggacatcga ggtgatgctg ctcacgcccg agcgcgacgt gcaggacttc
     4141 aagcgtgtgt tcgagacggc cacccgtctt ggcgcgcgca atgcgttgac gttgatcgat
     4201 attgcagacc gtccgctggc ggtcgaaaaa tttgggcaac tgtgcgaact ggcggcgcac
     4261 ttcggcattt cgtgcgcgct cgaattcgca gcgtggcttg gtgtcggcag cattcagact
     4321 gccaatgcga tcgtgagtga agcggggcaa accaacggtg caatgctgct cgatccgttt
     4381 cacctgattc gcagcggcgg gaaagtcagc gacatcgcag ccgtcgagcc ggcacgcatc
     4441 agatacgcgc agttctgcga cgccagcgga aaggcgcctg atacgaccgc gggcatctct
     4501 gaagaggcgc gttacgatcg cctgttgccg ggcgagggcg gcttgccgtt gcgcgagttt
     4561 gtcgctgcgc tacccgccgg cattcccttc ggtcttgaag tgccgaatcg caggctcgct
     4621 gagtcgctgg aactggacga acgattgcgt gcgaccctgg ctgccgcgaa acgggtcgcg
     4681 ggtgatcagt cctagaataa ttgcatgcag ggactcaccg gcggtatctt caacttctgc
     4741 gccaacctcg ccggcatcat cacgccgctc gtgatcggtc tgatcgtggc aaaaaccggc
     4801 tcgttcttct acgcgctggc atatgttggc gtcgcggcgc tggtcggagc actgtcatac
     4861 ctattcattg tgggcgaggt gcgacgtgtc gagatcgatg cctgaatccc acatacctga
     4921 aggagtgcag caatgatcag cggaaagact acccttattg cccacctcgg ctatccgacg
     4981 gagggtttca aagccccatt gatctacaac ccgtggttcg acaagaacgg catcgacgcg
     5041 gttgtggtgc cgatgggcgt actggcggac gactacaagg cattgctgcc tatgctgttt
     5101 cgcctgacca acatccgcgg cgcgctcgtc accatgccgc ataaggtgac gaccactggc
     5161 cttgttgacg agatgacgcc gaccgcccgc atcgcggggg catgcaacgc gatcctcaaa
     5221 cgcgaagacg gcacgctgct gggcgaccag ttcgacggtg cgggcttcgt gcggggcgtg
     5281 cagcgcaagg gccgcgagct gacgggtgcg cgcgtgctgg tacttggcag cggcggtgtc
     5341 ggatcagcga tcgctgcatc gcttgccggc gcgggcgtgg cggacatggc cttgttcgac
     5401 acggcgacgc agtcggtcga ggcgctcgcc ggccggctgc gtgagcatta tccggcgctt
     5461 cgcgtgagca ccggatcgaa agacccggcc ggtttcgatc tgatcgtgaa cgcgacgccg
     5521 ctcggcatga aagacggcga tccgattccg ttcgacgtcg agcgcatcgc gccgactacg
     5581 ttcgtcggcg aagtcgtgat gaaagcggaa tacacgccct tgctgcgggc ggcgaaagac
     5641 aagggctgtc cggtgcaggt gggcacggac atgctgtacg aaatgattcc agcctatctg
     5701 gagttcttcg gattcggtac ggccacgcct gaagagttac gcgcggtttc acagatcaaa
     5761 cagtaagcaa tcgaggtgga atgatgaaaa aagtactcgt gctcaatggg cccaatctca
     5821 acctgctcgg tcagcgcgaa cctgcgcagt atggctacga cacgctcgca acggtcgagc
     5881 agttgtgcaa ggaagcgggc gaacggctag gtgtagaagt ggaatgtcgt caatcgaacc
     5941 atgagggctt tctgatcgac tggatccacg aagccgggcg cgagatcgcc gcgggcaata
     6001 tgctcggcgt cgtgatgaat ccaggtgcgt tcacgcacac gtcggtggct ttgcacgacg
     6061 cgatcaaggg cgccaacgtg acgctgatcg agttgcacat ctcgaacgtc cacgcgcggg
     6121 aatcgttccg ccaccattcg tatatttcac ccgcggcgcg tggcatcgtc gtcggattgg
     6181 gggtgaaggg ctacggaatc gcaattgacg cgctagtgca gatttcagag cagaaatgag
     6241 cgggaaagcg gtaatcggca gccccgacga tcgacgaacg cgccttggcc cgctcctaca
     6301 agcgcttgcg atggacctct atgaaaagaa cgcgaactgc aaattgtgac gacatagctg
     6361 gcgacgccaa ttgcctgctc agtgaagggt gggggccctc gcaaacagag aacaaccatt
     6421 gcttgcgcat ctacatggga catcttcgcc acaagctgga gcgcgatccg gcacaccccg
     6481 agcacatcat caccaagtcg ggtgtcagct atcgaccggt tggcgcgcat tttcgatcag
     6541 ctgcatccgc gagtcgctga aattttacgc aactttagcg cggcttcagt tagcctgaaa
     6601 gcaatcagcg gaagacggaa gggcggtatg caacattccc tcgcacattt acggcggctc
     6661 ctcggacggg acgatgcccc tggccgaagg cgcccccggc gggagcgggc gaacgaagta
     6721 attgtgcgca tcacgctcga cgcgcagcac gcagctgcgc tgcgccatgc gttgatacgc
     6781 gattgcggcg gccagtcatg gacgatccgt cttgcaccac tgcgcggcac ggatcaaatg
     6841 cagcttgcgc tatatctgcc gaaggcagcg gtgaacgacg cgatgcggcg ggtttcgatc
     6901 atagcgcctg gcgccgcggt cgaccacatg ctcgaaattc cagacgttcc tagcgatgca
     6961 tggcgcgatc tgacacggga tagcgtaccg gagcgtgttg attcacgccg ccggctgccc
     7021 ggcgcggctc gatcgtcgca tgctatcgct gacctgctgc ggcgcgagca cgttctcctt
     7081 gatctggatg cgggcgatcg cgaggcactc tttgtggaaa taggccgcgt cgtggagcgc
     7141 tatcacggac tttcagcggc aacagtaaca gcggggctgc tggcgcgcga agcactggga
     7201 tcgactggac taggtcaggg tgtcgctgtt cctcacggcc agatcagtgg gctggatcaa
     7261 gaactggtgc tgtacgtccg cccggcgctg ccgatcctgt tcgatgcgcc ggacggatat
     7321 ccggtgagcg atgcgatcgt gctgctcgtg ccggagtggg cgaacacgat gcatcttcat
     7381 ttgctggcgg acgccgcgca gcacttttgc gaccaccact ttcgcgaacg gctccggctc
     7441 tgccgggatg cgaactctgt gctccagctg tttaaagact acgagacaca gggtgaggcg
     7501 atgcatcggt aggtgcggtc cgtcagcagg ccgtgcatca tccaaggcgg ctttagccaa
     7561 cctcaaggta caaagtatca atccggagtt ctcgatgttc cgcatgctga tccctgtcct
     7621 cgaccacggg ggcgcagcag aggctgcgcg ctttgcggcg tccatgtttc tggagcgctg
     7681 cctgaccgaa gtcgaaattc ttgaaatact cgaacccgtc gatcagggac gtgcggcagc
     7741 gtttcacacg cgaagctcac tggttcgcct ggaaaaacgg gccatgctga aggcgctggt
     7801 tgacgcccgg gcgattctcg aagaagccgg cgtgccgtat aaatggaaac ggatattcgg
     7861 gcaccgcacg aaagcgattg ctgcctacgc ggcaacaacc cagcccgacg tcatggtgat
     7921 cgacgccagt catatgggaa tctttcggag actcgcaacg ctcgccgctc tctcgatgcg
     7981 gacaagcacg ccggtaacga tggttcactg aatcatccga cgttcatcgg cagcagcacc
     8041 atctggctgc cttgttggtg cagccgcgtg tggatctcaa ctggcgtctg gttgtgcaac
     8101 catgccgtca ggaagtcctg ctgcaccgtt gacggcccgt aacgacaaag tcaggggcgc
     8161 tggagagaca tatgaaaccg acggcgatcg tattgcatgg gcctaccagc gcgggaaaga
     8221 gcagcttggc cagagcactc caggacagtt cggaaattcc cgtctttcac atatcgctcg
     8281 atgcattcgt cgaaatgtcg cgtagacgtg acatgcgatc agatgaagaa ctgaatcagg
     8341 cgctaaggct tcatcacctc aacttgcaat ccgtcttgaa gcgggtggcg gcgagtcact
     8401 tcgagatagt gctggatctt gtccttcggg atacatccgc cctggacgaa tgtatctctg
     8461 cgcttgcgcc tcgttgcacc tatgttatcg gcgtttcgtg cccactgaat gttttggagg
     8521 agcgcgaacg cggcaggtcg gaccgtggag agggaatggc caggtcgcaa ttcgggcatc
     8581 cggcctacac aatgccctac tcgatgcgcc tggatacatc gacttgcact cccgaagatg
     8641 gcgctcgtcg tatccgctcg tttatcaacg cgcagattga atgatcgctt acggtatcag
     8701 cgaacgtctt caacggctcg accccggccc ccagatgccg cggcgatgcc gggtttgtca
     8761 acgctgcgcg atgaccacgt gcgcctggat ctttccgtct accggtcccg cgccgaatcg
     8821 ctcggcaatc gcagttgcgg acgctgacgt ggcgtcgtcg acgcttgcac cggaacgggc
     8881 ctcaatctcg ttgcgcaagg gcgttccctg acaataggcg acggctggct cgcgagccga
     8941 tgccgcgcgg ctacgcgcca cgatagtctc gaagcgcggc gccgtactga aacccgcctc
     9001 cgcgaggtcg cgtgaaatca cttgtgggtc gaaatagccg tgcggcgtgc gcaccatgaa
     9061 aagtggtgga tccgtgggga acagagcagc aagcgccgcc gtgacggtat cggcaaactc
     9121 gttttcctca atccgatccc agacgttgaa caacagcacg ccaccgtgcc gaagtacgcg
     9181 ccgggcttcg caaaacgcga gggctttgtc cggaaagaac atgaccccga actggcagac
     9241 gacgacgtcg aaactcgcgt cctcgaacgg cagccgggtc gcgtcggcct gttgccactt
     9301 taccggcctg ggggtgccga ttgccgcggc acgatcgagc atcggctgat tcaggtcggt
     9361 cgcaaccagg tcgacatgcg tgcgcaatgc atacgccatc gcgcgggtca cgacacctgt
     9421 gccggcggcg gtctcgagca cgcgtgatgg cttgagaacc gcaacccggt tcgcgagatc
     9481 ggcggcgtag ggttcgaaga tcagcggaac gaggtacctg tcataaagct cggggatcga
     9541 tccggcgaag cgtgtgtcgg taccaaaccc gtccatgcgc ctgctccttt tattgcactt
     9601 gcggcaatct taggcgcaag tcaacaattt ctgaagaatc cgacgaacat gatcgggacc
     9661 gcccgatcga aaccgggaca cggctagcgc taaaacaatg agctagcctt tcacccattt
     9721 atgcctatat tcaacgtgga gcctgccggc attctgacct gagtcaggat tagcaccacc
     9781 ttcgttctcg tcgaggacaa acgcaagcgg gcccgttccg cccttcggca tatcaatgca
     9841 gcccggtgcg ctgcagctgg agtgacatgg agatgatgat ggcaacccca acctggaaga
     9901 tcagcggtca atactacgag acgtgcagtt gcgattttgt ctgcccgtgc ctgcccggac
     9961 aaatggcagt cagaccgagc aagggctcat gcacgtttgc catggcattc cagatcgatc
    10021 gcggccagca cgaagcggtg tccctcgatg gcctcggatt catcgtcctc ggattgacac
    10081 cagaagcaat ggccaagggg aactggtccg tgggccttat tgtcgatgag cgggccagca
    10141 cggagcagcg cgacgccata acggcaatta ccagcggcac ggcaggtgga ccgatggcgg
    10201 ctctatcgcc gctcgttgga tcgtttgtcg gggtcgagtc cgcggcgatc cgcttcgacc
    10261 gcaatgggac gaagtggtct gtcacatcgc cgcaattcgt cgacatggcc gctgaaggtg
    10321 cgatgggcat taatccgaac gcaaccgagc cgcttcatct ggataacacc ggtcatccgg
    10381 ccgccgatcg cgtggcactg gctcatgcct cgcgcagtca cgtccgcgcg ctcggctttg
    10441 agtgggatga cgtcagtggg cagaacaacg gtcaatatgc gcccttttcc tggcaaggtg
    10501 cgtaagctgg ctacctcttc tcgcgcaccc ggtcctggtt cgctcgcgag cctctcaagg
    10561 cgcgagcgcg ttctcattct gggttgcgtt gccgcggtca cgacgctgtc ctgggtgtat
    10621 ctcttctatc tcgatcatca gatgttgtcg gcaagacaac acgacaaaat gatgatggag
    10681 atgggcatgt cgatggcggc gccatggacg accgccgacg ttttcttcac gtttgcaatg
    10741 tgggtcgtca tgatgatggg catgatggct ggatcggcga tgcctgtatt gcttcttttc
    10801 gcgggggcac atgcccggcg gggcggaggc agggcgccgt tcgtcgttct gatgttcgcc
    10861 tcgggttacc tggccgtgtg ggttggcttc agcgcatgcg cgacactcgt gcagtgggca
    10921 ctgcaccatg ccgcgctgct gtcaccggag ttaggggcgt caagcccgca ggtgggcggt
    10981 gcgattcttt gcgcggctgg cctgtaccaa ttgacgccgt tcaagcgagc atgtctcatg
    11041 cactgccgaa gtccgctcgg gtttctaatg actcgctggc gcgatgggcc gtctggtgcg
    11101 ctgcagatgg gaatgcttca tggcgcctac tgcctcgggt gttgctgggc gctgatgtgc
    11161 gtcctgttcg ttgtaggggt gatgaatctt gtctgggtgg cggcgctagc gctgtttgtt
    11221 ttgctggaaa agatcggccc ggcaggcgcg gttctggcgc gcgcggccgg tgcggcgatg
    11281 atcgcggtcg gggttctgct gttcgccggc atgctttgaa tccttagcgt tgccgcaaag
    11341 gactgcaata gtgaccgccc agcgctagcc gcgacgggca ggtcgcggcc gatgctgttg
    11401 aaaagtcggt gaccgtctct tgcacgtcta tcgtgcctgg cagcgctggg caggtttaag
    11461 cgcctccttg agcaactaat aaggatgacc tggtttatcg ggaagtatct acctggtgct
    11521 tgccacgccc tcgcgactgc gcgttcaacg tcaggtagtc tgaagcagtg atgcgctgcg
    11581 tcatccatcg accgatggcg ctacgtgcga aaaggtctat catcgaaaag acgcatcgag
    11641 tgcctggctg tgcaacgcag ccgcccttcg ccttcgagga gaagaacaca tgacggagtt
    11701 ggtgaaggcg ctccccgctg cgacacccgg ccatccgcgc cggcagatcg tgtggctcta
    11761 cgcattacta ctgatcttca atctcgccgc atggcttgcg gccttcacgg cgtttcgcgg
    11821 ctatccgctg ctgatgggct cctgtttgct ggcttattcg ttcggattgc ggcacgccgt
    11881 ggacgcggac cacatcgccg ccatcgacaa cgtcacgcgc aagctcatgc aggccggcca
    11941 gcgtccgctg acggcaggac tgatgttctc gctcggccac tccaccattg tcgttctggc
    12001 gacgctcggc atcgccgtca ccgccatggc cttgcaaacc cggctaagcg gattcagggg
    12061 ggcgggcgcg atgatcggca cgctcgtgtc gacgtttttc ctgttcgcca tcgcgttgct
    12121 gaacctcatc gtgctgcgct ccgtgctgcg cgcgtttcgg cgcgtgcgcc gcggcgagcc
    12181 gtacgtcgac gaagatctcg acatgttgct cgccaatcgt ggttttctcg cgcgcatttt
    12241 ccggccgctg tttcgtctga tcacacgaag ctggcacatg tacccgcttg gcttcctgtt
    12301 cggcctcggt ttcgataccg cgaccgaagt cggcctgctc ggcatttccg cggcgggcgc
    12361 cgcgcaaggc atgtcgatct ggtcgatcct cgtgtttccg gtgctgttta ccgcgggcat
    12421 gacgctgatc gacaccaccg acagcatcct catgctgggc gcttacgggt gggccttcgt
    12481 gaagccggtc cgcaagctct actacaacct caccatcacg gcaatgtcgg tgctggtggc
    12541 gctgctggtc gggagtatcg aaggcttggg gctgctggct gacaagctgc atctgaatgg
    12601 cggattctgg caggcagtgg cggttctcaa cgataacttc ggcatgctcg gctacgcgat
    12661 tatcggcgta ttcgcggccg cctggctatt gtccgtggcc gtttacaaat ggcagcgaat
    12721 cggggagata gagatccatt cctgattaca acgcaatggc cgatctgtgt tgaccaaacc
    12781 cttaagggct atcgtcagag aattgaatag ggcgtgcgga tgcctttatc gcggtacgcg
    12841 tagtttcctg aataagcggt gaaaactggg ttatttcgta tcgctgacct atactcgaaa
    12901 aagtttttcg ggctcattgc ggtgggcacc attcaaacga aaacatgcag ccaatccgat
    12961 ccttttagcc gcgcctcacg cggaaatcat ttcgatcgat tgctggacac gtgactgccg
    13021 tgcggcgttc gccacgtatc tttgcttgtc taaaagaacg tgaacgcagt ggagacagat
    13081 catggcagaa gcagacaacg tcccctatgg gcgcaagact cagcacgcgc cggctctcac
    13141 ggaagtacac atcatctgga taaccgcggg tctcggctgc gacggtgatt cggtatccat
    13201 taccgctgcc acccagccaa gtatcgagga cgtgattctc ggcgcgattc cgggcttgcc
    13261 gaaggtccat ttacacaatc ccgtgctggc gtatgaaaac ggcgacgagt ttctcaaacc
    13321 gtttcatcag gccgcgcgtg gcgagctgga caacttcgtg ctggtgatgg aaggctcgat
    13381 tcccaatgag cgtatcaatc gcgagggtta ttgggccgcc ttgggcaccg atcccgatac
    13441 gggcgacccg atcacgattc cgcaatggct tgaccggctc gcgccgcgcg cgctggcggt
    13501 ggtcggcgcg ggaacctgcg cgacctatgg cggcattcac gccatgcagg gcaacccgac
    13561 cggctgcatg ggtctcgccg attatctcgg ctgggactgg aagtcaaagg cgggcctgcc
    13621 gatcgtcaac gtgccgggat gtccggtgca gccggacaat ttcatggaga ccctgctcta
    13681 tctgctgtat caactcgcgg gcctcgcgcc catgattcca ctcgacgacg cgttgcggcc
    13741 gaagtggctg ttcacccgca ccgtgcacga cggttgcgat cgtgcgggtt cgtacgagca
    13801 ggcgatcttc gcgaccgaat acggtagccc gaattgcatc gtcaagctcg gttgctgggg
    13861 accggttgtg caatgcaacg tgcccaaacg cggctggatg gcgggtatcg gcggctgtcc
    13921 gaatgtgggt ggcatctgca tcggctgcac gatgccgggc ttcccggaca agttcatgcc
    13981 gttcatggaa gagccgcccg gcgcggtgct gtcgtccaat ctgatcaaga gctatggccc
    14041 gctgattcgc agcctgcgca agctgaccag aaacacgctc aacgatgagc cgaaatggcg
    14101 tcacaaccgt tcggaactca ccaccggtta ccgtcgctga gcgggcggaa ttcgcgttgt
    14161 cagtgtcgtc agcattgtca gcaggagaag gtcatggcca tcagtaccgc acctgaagca
    14221 accagcagca ctcaggcagc gccgggcaag ctggtcgaaa tgaactggga tccgattacg
    14281 cggatcgtcg gcagtctggg gatttacacc aagatcgatt ttgcgaaccg ccgggtggcg
    14341 gagtgctata gcacgtcgtc gatctttcgc ggctatagca ttttcatgaa gggcaaggac
    14401 ccgcgcgacg cccatttcat cacgagccgg atctgcggca tctgcggcga caatcacgcg
    14461 acctgctcgg tctacgcaca gaacatggcc tacggcgtga agccgccgcc gatcgccgag
    14521 tggatcgtca atctcggcga agcggccgaa tacatgttcg accacaacat cttccaggac
    14581 aacctggtgg gcgtggactt ctgcgagaag agggtcaggg agaccaatcc gggcgtgtgg
    14641 gacaaggcgc aaaaaacgcc cgcgccgcac gcgaaagacc acggttaccg caccatcgcc
    14701 gacatcatgc gcgcgctcaa tcctttcgaa ggagaatttt accgcgaagc gctggtcatg
    14761 agccggctca cgcgcgaaat gttttgcctc atggaagggc g
//
LOCUS       DATASH010000766         3449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4281358,
            whole genome shotgun sequence.
ACCESSION   DATASH010000766 DATASH010000000
VERSION     DATASH010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4281358"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..1100)
                     /locus_tag="VHV99_20505"
     CDS             complement(90..1100)
                     /locus_tag="VHV99_20505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HEX3637820.1"
                     /translation="MTGLLFELAIMLVVILVAAELFTNALEHLGERLKISEGVTGSLF
                     AAVGTALPETLVPLLAIAGGTADSAVNQEIGVGAILGAPLMLSTLSTFLMTLAILGSR
                     GTKGWVTPERTGFTRDLNYFLCAFVLAAAAMYVPHEEMMVRALFSAALVGVYITYVVM
                     TFRASNKLVDAGHGTEAPGKMLLSRLGLPTNLATIAVQLALAVALLVWGAEGFIHGVR
                     GVSQALGVSPLLLSLLIIPIATELPEKVNSILWIRRGKDTLAFGNITGAMVFQGTLLP
                     AIGILLTPWTPRIEVLTGIVITLAAAAWLRINVRKGGVPVWALLVCGVLYASYLVITL
                     TR"
     regulatory      complement(1189..1371)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00080"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="yybP-ykoY riboswitch is a manganese riboswitch that
                     directly binds Mn; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="manganese"
                     /db_xref="RFAM:RF00080"
     gene            1539..2558
                     /locus_tag="VHV99_20510"
     CDS             1539..2558
                     /locus_tag="VHV99_20510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX3637821.1"
                     /translation="MSYQNRQWFYVKRPERRVSDEHYELRESELDDNLSSNEVLIRAR
                     IVSVDPYIRIQQHERNTYDVPHPLGIVQRAGVVGEVVASASPLFKQGDWVSAYSGWQL
                     YARCHHSELTKLNPDTAPVSTALGVLGMPGRTAWFGLMEAGRPRPGDTLVVSGAAGAV
                     GSLVAQFGKRAGCRVIGIAGGPDKCAFLTDTLKLDGAVDYRAHPTLEGLQSALQAATG
                     GVDVYFDNVGGMVTDAVIPLIKRRARIVICGAIAQYDGGLDTPDLAPRFLQHMLFQRA
                     TIQGILARDFVHRMDEMLQTVAPWVKNGEIVYQETYVDGFDALPNALNSLFEGKNLGK
                     LLVRV"
     gene            complement(2759..>3449)
                     /locus_tag="VHV99_20515"
     CDS             complement(2759..>3449)
                     /locus_tag="VHV99_20515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEX3637822.1"
                     /translation="NWGFSSTDPSYGNPKGIAGYIPAREAALYTSAKTILNTKAQSGY
                     RTVLHSQGISYFYNSAYPNNGWKPPASNPLIQATDDIKGVGNNKASATSALNSAVDVV
                     DIHPYFNSSEYVMLVQAFIDTLSTVVPNGPKKLWLTETNNGTDGTDAGQTAAFNQLKV
                     LMNNGSVQKAFWYVVRNGDPSNGEGDGYSIYDTNRNLIRPQLAAAIKAYTASIPKSQR
                     FLSGTYATAVT"
BASE COUNT          710 a         1029 c         1056 g          654 t
ORIGIN      
        1 ctggctgctg gaccgtctaa ttaaaggctg cttttgttgc ggccgtcgct gcggcctttg
       61 tgcggccatc cctgcctttc attactcgat tagcgagtca gcgtgatcac caggtaactg
      121 gcgtacaaca cgccgcacac cagcaacgcc cacaccggca caccgccttt gcgcacattg
      181 atccgcaacc acgcggcggc ggcgagcgtg atcacgatac cggtcaacac ttcaatgcgc
      241 ggtgtccacg gcgtcagcag aatcccgatc gcgggcagca aggtgccctg aaacaccatc
      301 gcgccggtga tgttgccgaa cgccagcgtg tccttgccac ggcgaatcca caggatgctg
      361 ttgaccttct cgggcagctc agtggcgatc ggaatgatca gcagcgacag cagcagcggc
      421 gagacgccga gcgcctgtga cacgccccgc accccatgga tgaagccttc cgcgccccac
      481 accagcaacg caacggccag cgccagttgc accgcgatgg tcgccaggtt cgtaggcaag
      541 ccaagacgcg acagcagcat cttgcccggc gcttcggtgc catggccggc gtcgaccagt
      601 ttgttcgagg cgcgaaatgt catcaccaca taagtgatgt acacgccgac cagcgccgcg
      661 ctgaacagcg cccgcaccat catctcttcg tgcggcacgt acattgccgc ggccgccagc
      721 acgaaggcgc acagaaagta attcagatcg cgcgtgaaac ccgtccgctc cggcgtgacc
      781 cagcctttgg tgccgcgcga gccgagaatc gcgagcgtca tcaggaaagt ggacagggtg
      841 gaaagcatca gcggggcgcc gagaatcgcg ccgacgccga tctcctgatt gacggcgcta
      901 tcggccgtgc cgccggcaat cgcgagcagc gggacgaggg tttcgggcaa tgcggtaccg
      961 accgccgcga acagcgaacc cgtcacgccc tcggagattt tcaggcgctc gccaagatgt
     1021 tcgagcgcat tggtaaagag ctcagccgcc accagaatga caaccagcat gatggcgagt
     1081 tcgaagagca ggccggtcat gcgcggctcc ccgcaaaagc aaagaggaag cggcacgacg
     1141 gacggccaag tggggagact ggcagggcac aacgatgttt cactgggcaa ctccgcggtc
     1201 ggacgtaatg acaaaccaat gacgcaccgc gacccgtccg accagaacga agcagtgcgc
     1261 caatggtctc gccaaaccga cccggtcgaa cgcgccatga cccgcaggtc aagtatgttg
     1321 acgcgctctc cttcaatgat tgaaggaagg ctactcccca gtgaccgggg gattctaacg
     1381 ggacgggcgc ctttcaacaa gcttgggatc tcgcgcatgt tccgatgcct ctcggcctcg
     1441 ccgcatccgt tcgacgatgc gagctcaggc gtgccgcggg cgtgcgcttg ccccactggt
     1501 aagatcactc atcgtctacg acagcagtga gtcttcacat gagttatcaa aatcgccagt
     1561 ggttttacgt caaacgtccc gaaagacgcg ttagcgatga gcattacgag ttgcgcgaaa
     1621 gcgaactcga cgacaatttg tcttcgaatg aggtgttgat ccgggcgcgc atcgtcagcg
     1681 tcgatccgta cattcgaatc cagcagcacg agcgcaatac ctacgatgtg ccgcatccgc
     1741 tcggtatcgt ccagcgcgcg ggcgtggtgg gtgaagtggt ggcgtcggcg agtccgctgt
     1801 tcaagcaggg cgactgggtc tcggcctata gcggctggca gttgtacgcc aggtgccatc
     1861 acagcgagtt gacgaagctc aaccccgaca cagcgccggt ttcgacggcg ctcggcgtgc
     1921 tcggcatgcc gggacgcacc gcgtggttcg gtttgatgga agcgggccgc ccgcgtccgg
     1981 gcgacacgct ggtcgtgtcc ggtgccgcgg gtgcggttgg ctcgctggtt gcgcagttcg
     2041 gcaagcgtgc cggatgccgc gtcatcggaa tcgcgggcgg cccggacaag tgcgcgtttc
     2101 tgaccgacac gttgaagctc gacggcgcgg tcgactaccg cgcgcatccc acgctcgaag
     2161 gcttgcagtc agcgctgcaa gccgcgaccg gcggcgtcga tgtctacttc gacaacgtgg
     2221 gcggcatggt caccgacgcg gtgattccgc tcatcaagcg ccgcgcgcga attgtgatct
     2281 gcggcgcgat cgcgcaatac gacggcggtc tcgacacgcc ggacctcgcg ccgcgcttct
     2341 tgcagcacat gctgtttcag cgcgcgacga tccaggggat tctggcgcgc gactttgtgc
     2401 accggatgga tgaaatgctg cagacggtcg caccgtgggt gaagaacggt gaaattgtgt
     2461 atcaggagac gtacgtcgat ggtttcgacg cattgccgaa cgcgctgaat agcctgttcg
     2521 aagggaagaa tctggggaag ctgctggtga gagtgtgatc agagccagcg ggatatctat
     2581 cccgctggct cagcctgttc gaagcgccaa tctcaccgta cggagattgg catcgaatag
     2641 agtgcgacgt gtgtttgcaa ggccttttgc aagacctgcc gggacccact gcatccagaa
     2701 gcgcgcgaag agattatccc tcaggcgctt tagctcgtgg aagcagtgga tcagctgcct
     2761 acgtcacggc cgtcgcatag gtccctgaca agaaccgctg cgatttggga atgctcgcgg
     2821 tataggcttt aatggcagcg gcgagctgcg gccggatcag attgcgattg gtatcgtaaa
     2881 tcgaataacc atcgccttcg ccattgctag gatcgccgtt gcgcacaacg taccagaacg
     2941 ccttctgcac agagccgttg ttcatcaaca ctttcagctg gttgaaagca gcggtctgtc
     3001 cagcgtccgt gccatcggtg ccattgttgg tttccgtgag ccagagcttt ttaggcccgt
     3061 tgggaacaac agtcgacaat gtatcgataa aggcctgcac cagcatgacg tattcactgc
     3121 tgttgaagta cggatggata tcgaccacgt caacagcgga attcagggcg ctggtagcac
     3181 ttgccttgtt attcccaacg cctttaatgt cgtcggttgc ctggatcagc ggatttgatg
     3241 caggcggctt ccaaccattg ttcggataag ccgagttgta aaaataagag attccctggc
     3301 tatgcaaaac cgttcgatag cccgactgcg ctttcgtatt caggatcgtc ttggcactcg
     3361 tgtagagcgc tgcctcgcga gccggaatgt agccggcgat tcccttcgga tttccgtaag
     3421 aaggatccgt cgaggaaaag ccccagttg
//
LOCUS       DATASH010000767         9963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_7050711,
            whole genome shotgun sequence.
ACCESSION   DATASH010000767 DATASH010000000
VERSION     DATASH010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 9963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9963
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7050711"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            435..1880
                     /locus_tag="VHV99_20520"
     CDS             435..1880
                     /locus_tag="VHV99_20520"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX3637823.1"
                     /translation="MATSSYTDTRLLINGEWCDAASGKTLDVVNPATGKPIGKVAHAG
                     IADLDRALAAAQRGFEAWRKIPANERAATMRKAAALVRERASDIGRLMTQEQGKPFAE
                     ARLEVLAAADIIEWFADEGRRVYGRIVPSRNLAAQQTVLKEPIGPVAAFTPWNFPVNQ
                     VVRKLSAALACGCSFLVKAPEETPASPAALLQAFVEAGVPAGTVGLVFGDPAEISSYL
                     IPHPVIRKVTFTGSTPVGKQLAALAGAHMKRATMELGGHAPVIVAEDADVALAVKAAG
                     GAKFRNAGQVCISPTRFLVHNSIREEFAAALVKHAEGLKLGDGLAEGTTLGPLANARR
                     LTAMSKVLDNARKTGAKVETGGERVGSEGNFFAPTVLTNVSLESDVFNNEPFGPIAAI
                     RGFDTLEEAIAEANRLPYGLAGYAFTKSFRNVHLLSQQVEVGMLWINQPATPSPEMPF
                     GGVKDSGYGSEGGPEAMEGYLVTKAVSVMAV"
     gene            1956..2555
                     /locus_tag="VHV99_20525"
     CDS             1956..2555
                     /locus_tag="VHV99_20525"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017328818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX3637824.1"
                     /translation="MTHPTLEPMQPTLTGHTVELRPLQQEHAQGLLDAAADGQLWNLK
                     LTVVPGPETIGGYIATALDGRAAGTVMPFVIVRRDTGAIVGSTRFWKIDRVNRKLEIG
                     HTWLGESVQRSAVNTEAKYLLLSHAFEAMQCVRVQFTTDELNEKSRAAILRIGAKQEG
                     IVRHERIMPDGRKRNSVRFSIIDEEWAQVKARLEGKLGR"
     gene            complement(2622..3638)
                     /gene="kdgT"
                     /locus_tag="VHV99_20530"
     CDS             complement(2622..3638)
                     /gene="kdgT"
                     /locus_tag="VHV99_20530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HEX3637825.1"
                     /translation="MKLKKAIDRIPGGLMLVPLLLGACVHTFAPGAGKYLGSFTNGLI
                     TGTVPILAVWFFCMGATIDLRATGMVLRKSGTLLVTKMVVAWIATLIAARFIPIDGVK
                     AGLFAGLSVLAITTSMDMTNGGLYAAVMQQYGSKEEAGAFVLMSIESGPLVSMIILGA
                     TGVAFFEPRLFVGAVLPFLIGFALGNLDGELREFFGRCVHPLIPFFGFALGSGIDLGV
                     IVKSGLPGIVLGLGVIVVTGIPLILADRWIAGGNGAAGLAASSTAGAAVANPAIIGEM
                     IPRFKPLVPAATAMVATACLVTAILVPILTALWVRRHHARDALREEFAGATQPPLKER
                     DVHV"
     gene            3955..4791
                     /gene="kduI"
                     /locus_tag="VHV99_20535"
     CDS             3955..4791
                     /gene="kduI"
                     /locus_tag="VHV99_20535"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006494530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HEX3637826.1"
                     /translation="MEVRQSINSEYAKTLDTAGLRKHFLVDQVFERDALQLTYSHIDR
                     IIVGGVWPATRAVELPASLGKAVGVNYLLERRELGAINIGGDGWVDVNGTRYAVRNEE
                     AIYIGKGAQAVAFGSDDAARPARFYLNCAPAHESYPTRTISLAQASPDTLGDPATSNR
                     RTIYKFIVPEVLPTCQLSMGMTKLEPGSLWNTMPCHTHERRMEVYFYFNIADDAAVFH
                     MLGEPDETRHILVHNEQAVISPSWSIHSGVGTRAYTFIWGMVGENQVFSDMDHIAVRD
                     LR"
     gene            4918..5673
                     /gene="kduD"
                     /locus_tag="VHV99_20540"
     CDS             4918..5673
                     /gene="kduD"
                     /locus_tag="VHV99_20540"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018434632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008678 - 2-deoxy-D-gluconate
                     3-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HEX3637827.1"
                     /translation="MNTFDLTGKVAIVTGSNTGLGAGMAVALATAGCDIVGVSRADAG
                     ATPALVKAAGRRFADVRADLASIAPVDDILRATIEAFGRVDVLVNNAGIIRRDDALDF
                     SEDDWDAVMDVNLKSVFFLSQAVARQFVTQQSGGKIINVASMLSFQGGIRVASYTASK
                     SGVLGLTRLLANEWAGHGINVNAIAPGYMATANTAALRGDAQRNDEILGRIPAGRWGT
                     PADLAGPVVFLASCASDYVHGHTLAVDGGWLAR"
     gene            5829..6713
                     /gene="kdgR"
                     /locus_tag="VHV99_20545"
     CDS             5829..6713
                     /gene="kdgR"
                     /locus_tag="VHV99_20545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HEX3637828.1"
                     /translation="MAAMGKQRKDDDVAHEPDPDSTGCMANDRGESASSVGRVFAILG
                     AIGDSGQIGISELSQKLGMSKTTVHRVIQTLKALGYVTQEIETERYRLTIRLFELGAK
                     ALESVDLVREADVEMRRIGQVTREAVHLGAFDEDAIIYIHKIDADYGLRMQSRIGRRN
                     PLHSTAIGKVLLAWMDPAEAREVLSHVEFRKSTQKTLACADAVLSILPRVREQGYGED
                     NEEQEEGLQCLAVPVFDRLGRVIAGLSISFPTMRCGADTKSHYVALLRQSGLAISTRL
                     GYREASAPEHLAIEQGSP"
     gene            6763..6888
                     /locus_tag="VHV99_20550"
     CDS             6763..6888
                     /locus_tag="VHV99_20550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637829.1"
                     /translation="MVQAAFTAGFGSTLRVKLRLWQAGDVAIWDNRAIQHYAVNS"
     gene            7636..7983
                     /locus_tag="VHV99_20555"
     CDS             7636..7983
                     /locus_tag="VHV99_20555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637830.1"
                     /translation="MNNSRSRWLSMAIVAGLGIGASSVALAHVDVGVNIGVPGVAYAP
                     APVYVPGPPVVVVSPGWHGDRYYDGHRYWERREWEGRHHEREWHDARGYRDGPHGHGD
                     DHGHGDDHGRDYH"
     gene            8064..8312
                     /locus_tag="VHV99_20560"
     CDS             8064..8312
                     /locus_tag="VHV99_20560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637831.1"
                     /translation="MVRSDPRRVTAQIDGTVISAEYSEQTGQLCLRQDGAVLREWFPP
                     HSWIAIASVAGARHWGTRPSDEDLQALLRNEMTLLRAQ"
     gene            complement(8317..8565)
                     /locus_tag="VHV99_20565"
     CDS             complement(8317..8565)
                     /locus_tag="VHV99_20565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637832.1"
                     /translation="MKSLINAVAIAVVLGAPIASFAQSNQAAPQNAQDTQAQVSGQQG
                     ARQTDTSGYGSASRGTWQAGYGSDTTVSSYSPPIHNVR"
     gene            complement(8769..9944)
                     /locus_tag="VHV99_20570"
     CDS             complement(8769..9944)
                     /locus_tag="VHV99_20570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HEX3637833.1"
                     /translation="MRTQVGIIGAGPAGLLLSHLLHLRGIDSVVLESRTREQIESTIR
                     AGVLEQGTMGLLTEAGVGARMKAEGALHHGFELAFEGKRRRIDLTGLTGHSITVYAQH
                     EVIKDLVAARVAADGALRFGVTETSLHGIDTDQPSIRYRYEGEACELQCDFVIGCDGS
                     QGVSRASIPEALRKDFERVYPFGWFGILCEGPPSSDELIYARHERGFALVSTRSQNVQ
                     RMYFQCDPKESVDSWSDDRIWAELHARVDSHDGQKIVDGKIFQKNIVGMRSFVSATMQ
                     HGRLFLAGDAAHIVPPTGAKGMNLAVADVRVLTQGLNAFYVENRTDLLDSYSETALKR
                     IWRAEHFSYWMTSMMHRIEGASPFEQQLQVAELEYVTTSRAAATAMAENYVGVAVAQ"
BASE COUNT         1953 a         3188 c         3080 g         1742 t
ORIGIN      
        1 taaagccaaa gcccttcgcg tcattgaacc acttcactgt accggttggc atgtcgattc
       61 ctcgtgcacc ttggttgatt ggattgcatt cgccgtcttt cgcctgccgc tttcgagtct
      121 tgccctgggt gctgccttcg cccacccctg gcatcatgga aagctgtcgg ctcccgcagg
      181 ttcctgctac tgctgcttga ctttacgcct gtttgagcgg cgccgctcaa gcaagcgttt
      241 ctccgcggcg acaaccgggg cagcaagacg cgtaccgcca ccggccatgg ccccagcgcc
      301 ggtgcgattg cgcacaatca gataagatga ttgctatcgg cagtccatcc atggccgcgc
      361 aagtccagct tatcgctccg cgatccgcct cacgaccgcg cgacctatcg ctttcccact
      421 aacaggaacc cagcatggcc acttcgagct ataccgatac ccgacttctg atcaacggcg
      481 agtggtgcga cgccgccagc ggcaaaaccc tcgacgtcgt caatcccgcc accggcaagc
      541 ccatcggcaa ggtggcccat gccggcatcg ccgatctgga ccgggcgctg gccgccgctc
      601 agcgcggctt tgaagcatgg cgcaagattc cggccaacga gcgcgccgcg accatgcgca
      661 aggccgccgc actggtgcgc gagcgcgcct ccgatatcgg ccgcctgatg acgcaggaac
      721 agggcaagcc gttcgccgaa gcgcgcctcg aagtgctggc cgcggcggac atcatcgagt
      781 ggttcgccga cgaaggccgc cgcgtctacg gccggatcgt gccgtcgcgc aatctggcgg
      841 cgcaacaaac cgtgctcaag gaaccgatcg gcccggtggc cgccttcaca ccgtggaatt
      901 tcccggtcaa tcaggtcgtg cgcaagctga gcgcggcgct cgcgtgcggc tgctcgttcc
      961 tcgtcaaagc gccggaggaa accccggcat cgccggcggc gctgctgcag gccttcgtcg
     1021 aggcaggcgt accggcgggc acggtcggcc tcgtgttcgg cgatccggct gagatttcga
     1081 gctacctgat tccgcatccg gtgatccgca aggttacctt caccggttcc acgccggtcg
     1141 gcaagcaact ggccgcgctg gccggtgcgc acatgaagcg cgcgacaatg gaattgggcg
     1201 gtcacgcgcc tgtcatcgtg gccgaagatg ccgacgtggc actggccgtc aaggccgccg
     1261 gcggcgcgaa gttccgcaat gcaggccagg tctgcatttc gcccacgcgc tttctggtgc
     1321 acaacagcat ccgcgaagaa ttcgctgcgg cgctcgtcaa gcacgccgaa ggcctcaagc
     1381 tcggcgacgg cctggcagaa ggcaccacgc tggggccgct cgccaacgcg cgccgtctca
     1441 ccgcgatgag caaggtgctc gacaacgcgc gcaagaccgg cgccaaggtc gagacaggtg
     1501 gcgaacgcgt cggctcggaa ggcaacttct ttgcgccgac cgtgctgacc aacgtgtcgc
     1561 tcgaatcgga cgtgttcaac aacgagccgt tcggcccgat cgccgcgatc cgcggtttcg
     1621 acacgctgga agaagcaatc gctgaagcga atcgtctgcc gtacggtctg gcgggttatg
     1681 cgttcacgaa gtcgttccgc aacgtgcatc tgctgtcgca acaggtggaa gtcgggatgt
     1741 tgtggatcaa ccagcctgcc acgccctcgc cggaaatgcc gtttggcggc gtgaaggatt
     1801 cgggctatgg ctcggaaggc ggtccggaag caatggaagg ctatctggtc accaaggcgg
     1861 tttcggtgat ggcggtttaa gacggggctt tcagactttt atcggctcac tgtgaaaggc
     1921 ccgcgaacgc gcgttcgtgg gccttttttc ctgttatgac ccaccccacc ctcgaaccta
     1981 tgcaacccac gctgacggga cacacggtcg aactcaggcc gcttcaacag gaacacgcac
     2041 aaggtctgct cgacgctgcc gcggacggtc agttgtggaa cctgaagctg accgtcgtgc
     2101 cgggccccga aaccattggc ggctatattg caaccgcact cgatggacgc gcggccggga
     2161 ccgtgatgcc gtttgtgatc gtgagacgcg atacgggggc gattgtcggc agcacgcggt
     2221 tctggaaaat cgaccgggtg aaccggaagc ttgaaatcgg ccacacgtgg ctgggcgaat
     2281 cggtgcagcg ctcggcggtg aataccgagg cgaagtatct gctgttgagt catgcgttcg
     2341 aagcgatgca gtgcgtgcgc gtgcagttca caactgacga gctcaacgag aaatcaagag
     2401 ccgcgattct gcggatcggc gcgaagcagg aagggattgt ccggcacgag cggatcatgc
     2461 cggatgggcg taagcgcaat tcggtgcgct ttagcatcat cgatgaagag tgggcgcagg
     2521 tgaaggcgag gctggagggg aagctggggc ggtgagcccc gtctcacgat actcgcttca
     2581 cgccgccgcg ttcccgcaca atccagccgc ctcccactct ctcaaacgtg cacgtcccgc
     2641 tccttgagcg gcggctgcgt tgcccctgca aactcctcgc gcaatgcatc gcgcgcatga
     2701 tgccggcgca cccacagcgc ggtcagaatc ggcacaagaa tcgcggtcac gagacatgcc
     2761 gtcgccacca tcgccgtcgc tgccggcact aacggcttga agcgcggaat catctcgccg
     2821 atgatcgccg ggttcgccac cgcggcaccc gccgtcgacg aagccgccaa ccccgctgcc
     2881 ccattgccgc ccgcgatcca gcggtccgcg agaatcagcg gaatacccgt cacgacgatc
     2941 accccaaggc ccagcacgat ccccggcaag ccgctcttga cgatcacgcc gaggtcgatg
     3001 ccgctaccga gcgcaaagcc gaagaacggg atcagcggat gcacgcaacg accgaagaat
     3061 tcgcgcaact cgccatccag attccccaac gcgaagccga tcaggaacgg cagcaccgca
     3121 ccgacgaaaa gccgcggctc gaagaacgcg acaccggtcg caccgagaat gatcatgctg
     3181 acgagcggcc ccgattcgat cgacatcagc acgaacgcgc ccgcctcctc cttgctgccg
     3241 tattgctgca tcacagccgc atagagaccg ccgttggtca tgtccatcga ggtcgtgatc
     3301 gcgagcaccg agaggccggc gaacagccct gccttgacac cgtcgatcgg aatgaagcgg
     3361 gcagcgatca gcgtcgcgat ccaggccacc accattttgg tcacgagcag cgtgcccgac
     3421 ttgcgcagca ccatgcccgt cgcccgcagg tcgatcgtcg cgcccatgca gaagaaccac
     3481 accgccagaa tcggcacggt gccggtgatc aagccgttgg taaacgagcc gagatatttt
     3541 cccgcacccg gcgcaaacgt gtgaacgcaa gcgccgagca gcaacggcac cagcatcagg
     3601 ccgcctggaa tgcggtcgat ggccttcttg agcttcatgc ctcctccatg gactttgcgt
     3661 cccgttatcg ggtggatcac ccgagggtaa ttgtcccgtt cttacgccgg gcgactccct
     3721 ttcggcgcaa tatagcatca cgataggaac aacgttccga ttttgataga cttccggctc
     3781 atcgtttacc ctttgccatc cgcaagcctt gtcacgcttg atgcagcggt cagatatttt
     3841 cggaccgatg ttccgtatta attattctgc gctacagtcg agagcagaac ggcgctgcaa
     3901 tacggcacag atacggcaca acaagacgcc ccattccgac cggagaaaag aaccatggaa
     3961 gtgagacaga gcatcaacag cgaatacgcg aagacgctgg acaccgccgg actgcgcaaa
     4021 cactttctcg tcgaccaggt gttcgagcgg gacgcgcttc aactcaccta tagccatatc
     4081 gaccgcatca tcgtcggcgg cgtgtggccg gccacgcgcg cggtcgaact gcccgcatcg
     4141 ctcggcaagg cggtcggcgt caactatctg ctggagcgcc gcgagctcgg cgcgatcaac
     4201 atcggcggcg acggctgggt cgacgtgaac ggcacgcgct atgcggtgcg caacgaagag
     4261 gcgatctaca tcggcaaggg cgcacaggcc gtcgcgttcg gcagcgacga cgccgcgcgc
     4321 cccgccaggt tctatctgaa ctgcgcgccc gcgcacgagt cgtatccgac ccgcacgatc
     4381 tcgctcgcgc aggcgtcgcc cgacacgctc ggcgacccgg ccacgagcaa ccgccgcacc
     4441 atctacaagt tcatcgtccc cgaggtactg cccacctgcc agctctcgat gggcatgacc
     4501 aaactcgaac ccggcagcct ctggaacacg atgccgtgcc acacgcacga gcgccgcatg
     4561 gaggtgtatt tctatttcaa catcgcggac gacgcggccg tctttcacat gctgggcgag
     4621 cccgacgaaa cgcgacacat tctcgtgcac aacgaacagg cggtgatctc gccgagctgg
     4681 tcgattcact ccggcgtcgg cacgcgcgcc tacacgttca tctggggcat ggtcggcgaa
     4741 aatcaggtgt tcagcgatat ggatcacatc gcggtccgcg acctgcgttg aaggcgagca
     4801 ggcgcctcac tcagaaccgc gcgggcgccg caagcaccac gcctacccag gcacacccgg
     4861 acatacaagc acccgtggca ccacaggcaa cgcgaacgac atagggacct acgcaacgtg
     4921 aacaccttcg atctcaccgg caaggtcgcc atcgtcaccg gcagcaacac gggcctcggc
     4981 gccggcatgg ccgtcgcgct cgccacggct ggctgcgaca tcgtcggcgt gagccgcgcg
     5041 gacgccggcg cgacgcccgc gctcgtcaaa gcagccgggc gccgcttcgc ggacgtgcgc
     5101 gccgacctcg cgtcgatcgc tccggtcgac gacattctgc gcgccacaat cgaagcgttc
     5161 ggccgcgtcg acgtgctcgt gaacaacgcg ggcatcatcc gtcgcgacga cgcactcgac
     5221 ttcagcgaag acgactggga cgccgtcatg gacgtcaacc tgaagagcgt gttctttcta
     5281 tcgcaggccg tggcgcggca attcgtgacg cagcaaagcg gcggcaagat catcaacgtc
     5341 gcgtcgatgc tgtcgttcca gggcggcata cgcgtagcgt cctatacggc ttctaaaagc
     5401 ggcgtgctcg gtctcacgcg attgctcgcg aacgaatggg ccgggcacgg catcaacgtg
     5461 aacgcgatcg cgccgggcta catggcgacc gcgaacacgg cggcgctgcg cggcgacgcg
     5521 cagcgtaacg acgaaattct cggccgcatt ccagccgggc gctggggtac gccggcggat
     5581 ctcgcgggac cggtcgtgtt cctcgcctcg tgcgcgtcgg actatgtgca cggccatacg
     5641 cttgcagtag acggcggctg gctggcgcgc tagcgtggca caagcagagg tcgcgcggat
     5701 cgcgggacgc ggtatgctct cggcgccggg gtctggtcgg cactgatcgg catcgggctg
     5761 taatcaggtt tcgagtgcgt tttttcagcg cgttttgagc agacaggcat caacagggaa
     5821 tcagagaaat ggcagcaatg ggcaagcagc gcaaggacga cgacgtcgca cacgagccgg
     5881 acccggacag cacgggctgc atggccaacg accgtggcga atcggcttcg tcggtcggtc
     5941 gggtcttcgc gattctcggc gcgatcggcg acagcggaca gatcggcatc agcgaactgt
     6001 cgcagaagct cggcatgtcg aagaccaccg tgcaccgcgt gatccagacg ctgaaggcgc
     6061 tcggctatgt cacgcaggaa atcgaaaccg agcgctaccg gctgacgatc cgcctgttcg
     6121 aactcggcgc gaaggcgctg gagagcgtcg atctggtgcg cgaggccgac gtcgagatgc
     6181 gccgcatcgg ccaggtcacg cgcgaggcgg tgcacctcgg cgcattcgac gaagacgcca
     6241 tcatctacat ccacaagatc gacgccgatt atgggttgcg catgcagtcg cgaatcggcc
     6301 gccgcaatcc gttgcacagc acggcgatcg gcaaggtgct gctggcgtgg atggacccgg
     6361 ccgaagcacg cgaggtgctc tcgcacgtgg aatttcgcaa gtcgacgcaa aagacgctcg
     6421 cctgcgccga cgccgtacta agcatcctgc cgcgcgtgcg cgagcaaggc tacggcgagg
     6481 acaacgagga acaggaagaa ggacttcaat gtctcgcggt gccggtattc gaccgcctgg
     6541 gccgcgtaat cgccggcctg tcgatttcgt ttccgaccat gcgctgcggc gccgacacga
     6601 aatcgcacta cgtcgcgctt ctcaggcaat cggggctcgc gatctcgacg cggctcggct
     6661 atcgcgaggc gagcgcgccg gagcatctgg ccattgagca gggttcccct taaacccgaa
     6721 cgcgccgcta gagccgcctc aatcggggcc caagcggccc gcatggttca agcggccttc
     6781 actgccggct tcggttcgac gctgcgggta aagctgcggc tctggcaggc aggcgacgtc
     6841 gcgatctggg acaaccgcgc gatccagcac tacgccgtca actcctgagg ttgaggacga
     6901 ggcagtcagc gtgccgtgag gcacgcaggg cggaagtcgt tagctgaagg cggcagtggc
     6961 gcaaccgcgc cgggcgccga tcagctcgac agtgctttca tcgatctaac cagtgttgac
     7021 ggcgcacgta aaagactcgt tttttctgtg cttatcacgc actcggcaat aactatcgcg
     7081 cgcggacgcc atgacgtcga aatcgtcttg atcgaggccg taccttccaa gtctgcaact
     7141 gaatgtatcc gtctgacgta cggccgacga tgggctgcgt cgtgcatgtt tctgttgttc
     7201 acacgtaggc gcgccggttt gccggagccc gacgggtaca tgacggcctc acacttttca
     7261 tagcgtgctc aacgggttgg ccactgactg ccggcatgcc agtggaacga ctaatcgcag
     7321 cgctgcaacg gattcccgca ctctgctgtg tacccagccg acacacgcag gaatcgacac
     7381 gtcctgttac acacgaacac accggtaata ccgccgctca gtagagttcg atcacacgca
     7441 atcgggagag caacatcatg aaacgaattt tcacgctggt tctattggct atcaccctcg
     7501 gcagtgcatt gggtggttgc atcttcgtcc ctgagggtgg ttatcacgat catgaccact
     7561 atcacgatca cgaccattac taagcggcgc ccgcagagcg cactccacta tcaatcgaac
     7621 aaccgggaat aaaacatgaa caattcacga agccgctggc tgtccatggc aatcgttgcg
     7681 ggtctgggga tcggggcatc ttctgtcgcg ctggcgcacg tcgatgtagg cgtgaacatc
     7741 ggcgtgccgg gcgtggccta cgcgcccgcg ccggtctacg ttcccgggcc gcccgtggtg
     7801 gtagtgagtc ccggctggca cggcgaccgc tattacgacg gccaccgcta ctgggaacga
     7861 cgcgagtggg aaggccgcca tcacgagcgc gaatggcatg atgcgcgcgg ttatcgcgac
     7921 ggcccgcacg gccatggcga cgatcacggc cacggcgacg atcacggccg cgactaccac
     7981 taatgacgca tgggccgtgg cggtgtcatc aggccgcgcg cccgcatacg cattcgcgcc
     8041 acaccttttg caggagcgac gatttggtac gcagcgatcc acggcgagta acggcgcaaa
     8101 tcgacggcac agtcatctcc gccgaataca gcgaacagac cggccaattg tgtttgcgtc
     8161 aggacggcgc cgtgcttcgc gaatggtttc cacctcattc gtggatcgcg attgcgtccg
     8221 tcgccggcgc gcggcactgg ggtacgcggc ctagcgatga ggatcttcag gccttattgc
     8281 gcaatgagat gaccctgttg cgcgcgcaat gaccgcttaa cgtacgttgt ggatcggcgg
     8341 cgaataggag cttaccgtcg tgtcgctgcc gtaaccggct tgccatgtgc cgcgactggc
     8401 agaaccgtaa ccgctggtat cggtctgacg cgcgccctgc tgcccggaaa cttgcgcttg
     8461 cgtatcctgc gcgttttgcg gggccgcctg attcgattgc gcaaacgaag cgatcggagc
     8521 gccaagaaca acggcaatag ccacagcgtt gatgagcgat ttcatgaatc cacctccaga
     8581 gattgcctta tacgcgttgc agatgtgacg cgttagtacg gctgaagaga gtgtagaggt
     8641 ggattagcgg cggataaatc ctacattcct gaattcagtg tttctgttga cacgacaatg
     8701 gtgaacgctc actcatcagg tttgccgatg cgggttgggg caccgcgcgc cgcggcgcca
     8761 ctcgcggatt attgcgcgac cgcgacgcct acatagttct ccgccatcgc ggtagctgcc
     8821 gcacgcgacg tggtcacata ttcgagctcg gctacttgca gttgttgctc gaacggcgag
     8881 gcgccctcga tgcgatgcat catgctcgtc atccagtacg agaaatgttc cgcgcgccag
     8941 atgcgcttaa gcgcggtttc actgtaactg tcgagcagat cggtgcgatt ttccacgtag
     9001 aacgcgttca gcccctgggt caacacacgc acgtcggcga cggcgaggtt catccccttg
     9061 gcgccggtgg gcggcacgat atgcgcggcg tcgccggcga ggaagagccg gccatgctgc
     9121 atggtggccg acacgaaact gcgcatgccg acaatgttct tctggaaaat cttgccgtcg
     9181 acgattttct ggccgtcgtg cgaatcgacg cgtgcgtgca gctcagccca gattcgatcg
     9241 tcggaccagc tgtcgacgga ttccttggga tcgcattgga aatacatgcg ctgcacgttc
     9301 tgcgagcggg tgctgaccag tgcgaaaccg cgctcgtgcc gcgcgtagat cagttcatcc
     9361 gacgagggcg ggccttcgca cagaatgccg aaccagccga atggatagac gcgttcgaag
     9421 tccttgcgca aagcctcggg aatcgacgca cgcgagacgc cttgcgagcc gtcacagcca
     9481 atcacgaagt cgcattgcag ttcgcatgct tcgccttcgt agcgatagcg gatcgaaggc
     9541 tgatcggtat cgataccgtg cagcgacgtc tccgtcacgc cgaaacgcag tgccccgtcg
     9601 gccgccacgc gcgcggcgac caggtccttg atgacttcgt gttgcgcgta caccgtgatc
     9661 gaatgaccgg tgagacccgt cagatcgatg cgccgccgct tgccttcgaa cgccagttca
     9721 aagccgtgat gcagtgcgcc ctcggccttc atgcgcgcgc cgacgccggc ttcggtgagc
     9781 aggcccatcg tgccctgttc gagcacaccg gcgcggatgg tcgattcgat ctgttcgcga
     9841 gtacgcgatt cgagcacgac ggagtcgatg ccgcgcaagt gaagaagatg ggaaagaagc
     9901 aggcccgcag gcccggcacc gatgatgcca acttgggtgc gcatgagttg tctcctgaat
     9961 gcg
//
LOCUS       DATASH010000768         1810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_522155,
            whole genome shotgun sequence.
ACCESSION   DATASH010000768 DATASH010000000
VERSION     DATASH010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522155"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            332..911
                     /locus_tag="VHV99_20575"
                     /pseudo
     CDS             332..911
                     /locus_tag="VHV99_20575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1027..>1810)
                     /locus_tag="VHV99_20580"
     CDS             complement(1027..>1810)
                     /locus_tag="VHV99_20580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX3637834.1"
                     /translation="NSTRTGAGTDSLSEAEVSARIKPFAQVDIRDANAPRVYKSGEEV
                     YKAVCSACHAAGTAGAPKFTNTADWAPRISQGFDTLWHTALAGKGAMPPRGGTSPDDY
                     SDFEIARAVVYMANNSGASFPEPAQPAAGAAAAASGAAAAAPAGASDAGAAQAAAAMA
                     AMASVPQAAAPAAGGTQSADASQAGKALYQQVCQACHAAGVLNAPKFGDKDAWAPRLK
                     EPMDTVYNYALHGKGAMPPKGGSTASDADVKAAVDYMVSAAK"
BASE COUNT          270 a          610 c          580 g          350 t
ORIGIN      
        1 gaaggcgctg ttgcagaagc cttgagcgtg gagacgaacg tgagccagct ggacgttttc
       61 tgttcgcctg cctgtaagac ccagcgcgcc gggcacagct acttgcttgc aagacccgac
      121 gcgccggacg ctcccacgcg ctcgtaaccc cacgcgccgg gcaaccccca gccgcgtgac
      181 agacgtcact cgctgcacgc tcacacgctt gcaagacccc gcgcactgca ggtgcatcgg
      241 cgattttccc gtgtgcgcac gctacagtgg cacggcccgt taattggacg ttggtcagac
      301 gttgatcggg caccaatcgg agaccgttcg catgtccatg ccgccgctcg cgcactatcg
      361 cctcgcgctc gaaacctaca tcgacgcgaa agacaacacg cgtgctaacc gcatcgccga
      421 ggcttttgca ccggatgcgg tcctcactat ttcgctcgct acgtcgaata tcgcgttccc
      481 ggcgcgcacc gtcggcactg atgcgatcgc gaaaacgctc gtcaccgatt tcggcgctca
      541 atatgcgcag tgccgcacct actacgtgtg cgatttcgct ctcggtgaat gacggcacga
      601 tcgacgcgtt gccctggctg gtggtgatgc gcgaacgggc aaccggcgcg ttgcgcgtcg
      661 gacacggggt gtatcggtgg agtttcgatg tgatgccggc cgtcacggcg ttccatatcc
      721 acatcgctcg catggacgtc gtgcccgatg ccgacggcaa gttgcttgaa gcgttacaag
      781 atggattgag ctatccctgg ctcgcgcccg gtgatctgca cgcgcgtttt gcgtcgctcg
      841 cgcgcagcac gccggccttg gcctttgtcc aggcgttcag ccgtcccgca gttgcattgg
      901 caggcacctg acctggcggc gcgtaaagcg cgtcccgatt ccccatgaaa aaatccctgc
      961 ccgtgtcgcc acggtgcagg gatttttgcg ttttgcgcaa tcgccggagg cggtcccgga
     1021 agcgccttat ttcgccgcgc tgaccatgta gtcgacggcg gccttcacgt ccgcatcgga
     1081 ggcggtcgag ccgcctttgg gcggcattgc gcccttgccg tgcagcgcgt agttgtagac
     1141 cgtgtccatc ggttccttca ggcgcggcgc ccacgcgtcc ttgtcgccga atttcggtgc
     1201 gttcagcacg ccggccgcgt gacatgcctg gcaaacctgc tgatacaacg ccttgccggc
     1261 ttgcgaggcg tcggcgcttt gcgtcccgcc tgctgctggt gccgccgctt gcggcacgct
     1321 cgccatggcc gccatcgcgg cagcggcttg tgcggctcct gcgtccgaag cccccgccgg
     1381 cgccgcggcg gcagcgcccg aggcggcggc cgccgcaccc gctgccggtt gcgccggttc
     1441 agggaagctc gcgccggaat tgttcgccat gtaaaccacc gcgcgagcga tttcgaagtc
     1501 gctgtagtcg tccgggctgg tgccgccgcg cgggggcatc gcgcccttgc cggcgagcgc
     1561 cgtgtgccac agcgtgtcga agccctgcga gatgcgcggc gcccagtcgg cggtattggt
     1621 gaatttcggc gcgcctgccg tacctgccgc gtgacacgcg gagcatacgg ccttgtagac
     1681 ctcttcgccg gacttgtaga cacgcggtgc attagcgtcg cgaatgtcga cttgggcgaa
     1741 cggcttgatg cgcgcgctga cctcggcctc ggaaagactg tctgtgccgg cgccggtgcg
     1801 cgtcgaatta
//
LOCUS       DATASH010000769         4335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_323857,
            whole genome shotgun sequence.
ACCESSION   DATASH010000769 DATASH010000000
VERSION     DATASH010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4335
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323857"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1415)
                     /gene="uvrC"
                     /locus_tag="VHV99_20585"
     CDS             complement(<1..1415)
                     /gene="uvrC"
                     /locus_tag="VHV99_20585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEX3637835.1"
                     /translation="MTEPEATDVFEPKKVLAQLPHLPGVYRYYDTQGTVLYVGKARDL
                     KKRVSSYFTKTLLSPRIAMMVTRIARIETTVTRSEAEALLLENNLIKALTPRYNILFR
                     DDKSYPYLKLTGHKFPRMAYYRGSVDRKNQYFGPFPSAWAVRESIQILQRVFQLRTCE
                     DSVFNNRTRPCLLHQIGRCTAPCVAAISEEDYARDVANASRFLLGRQGEVMKELEQKM
                     HAFASELKFEQAAAVRNQMSSLSTVLHQQAIEVGGDSDVDILAVVALGGRVCVNLAMV
                     RGGRHLGDKAYFPAHVESALTVAEGGLEEGGGDDLLIDVADGAGSVSDESAIGASALA
                     DAADALAIAGEMPSEEDGDGDAAVEADALDDVLDADADADADADADADADADSKVEPS
                     AQRKGRAAAGGIESEVLEAFIAQHYIGNRVPPVLVVSHAPATRELVDVLIEQAGHKVT
                     VLRQPQGQRRAWLAMAEQNARL"
     gene            1683..2876
                     /gene="earP"
                     /locus_tag="VHV99_20590"
     CDS             1683..2876
                     /gene="earP"
                     /locus_tag="VHV99_20590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HEX3637836.1"
                     /translation="MSTAAPPSEPATAAPTAIACDIFCAVIDNFGDIGVCWRLARQLA
                     SEHGWQVRVFVDDLHAFQKLCPSLALDRARQAVGGIVIEHWHEPAHAGDTLEVADVVI
                     EAFACELPSIYVAAMARRERAPVWFNLEYLSAEDWVADFHLRPSPHPRYPLTKTFFFP
                     GLGPGTGGVLKERHLDTARAAFEASQPTREAWWLTTTGRAPPPSTATIVSLFAYENPA
                     VDSLLEQWRDSAEPVVLLVPEGRISGAVARFFGLSSFAAGSHAERGGLSVHALAFTEQ
                     PGYDALLWASDINFVRGEDSFVRAQWAAKPFVWHIYPQADDAHLPKLDAALAHYGRTL
                     PSNARDALARFWHAWNSAGRPDWAEFQHHRAALKQRATDWAGELAQVGDLAGNLALFA
                     KSQLK"
     gene            3337..3894
                     /gene="efp"
                     /locus_tag="VHV99_20595"
     CDS             3337..3894
                     /gene="efp"
                     /locus_tag="VHV99_20595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEX3637837.1"
                     /translation="MKTAQELRTGNVVMIGADAMVVQKAEYNKSGRNSAVVKMKFKNL
                     LTGAGMESVYKADDKFDVVVLERKEVTYSYFADPMYVFMDADYNQFEVESEMMGDALH
                     YLEDGMACEVVFYNEKAISVELPTTLVREITYTEPAVKGDTSSGKVLKNAKLNTGFEL
                     QVPLFCAIGDKIEIDTRTHEYRSRA"
     gene            4184..>4335
                     /locus_tag="VHV99_20600"
     CDS             4184..>4335
                     /locus_tag="VHV99_20600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEX3637838.1"
                     /translation="MNNDIAEGKWKQMVGKAKAAWGELTDDELTKAEGHADRLAGLIQ
                     ERYGKTR"
BASE COUNT          839 a         1410 c         1294 g          792 t
ORIGIN      
        1 agccgcgcgt tctgctcggc catcgcgagc catgcacgcc gttgaccttg cggctggcgc
       61 agcacggtca ccttgtggcc ggcctgctcg atcagcacat cgaccagttc gcgcgtggcc
      121 ggcgcgtggc tgaccacgag caccggcggc acgcgattgc cgatgtagtg ctgcgcgatg
      181 aacgcttcca gcacttcgga ttcgatgccg ccggcggccg cacggccttt gcgttgcgcc
      241 gagggttcga ctttggagtc ggcgtcggcg tcggcgtcgg cgtcggcgtc ggcgtcggcg
      301 tcggcgtcgg cgtccaacac atcatccagc gcatccgcct cgaccgcagc gtctccatcg
      361 ccgtcttctt ctgagggcat ctccccggca atggcgagcg cgtcagccgc atcggccaat
      421 gcgctcgcgc cgattgcgct ttcgtcagat accgagccgg cgccgtctgc aacatcgatc
      481 agcagatcat cgccgccacc ctcttcaagc ccgccctctg cgaccgtcaa cgcgctttcc
      541 acatgcgcgg gaaaatacgc cttgtcgccc agatgccgtc cgccgcgcac catcgcgaga
      601 ttcacgcaca cgcgcccgcc tagcgcgacg acggcgagaa tatccacatc gctatcgccg
      661 ccgacttcga tcgcctgctg atgcagcacc gtcgacagcg aactcatctg attgcgtacc
      721 gcagcggcct gctcgaactt caactcgctc gcgaacgcgt gcattttctg ctcgagttcc
      781 ttcatcactt cgccctgccg gccgagcaga aagcgcgacg cattggcgac atcgcgcgcg
      841 tagtcttctt cgctgatcgc cgcgacgcac ggcgccgtgc agcggccaat ctgatgcagc
      901 agacacggcc gcgtgcgatt gttgaacacg gagtcttcgc aggtgcgcaa ctggaacaca
      961 cgctgcagga tctggatgct ctcgcgcacc gcccacgcgc ttgggaacgg gccgaagtac
     1021 tgattcttgc gatccaccga gccgcggtaa tacgccatgc gcggaaactt gtggccagtc
     1081 agcttgagat atggatacga cttgtcgtcg cgaaacagaa tgttgtaacg cggcgtcagc
     1141 gccttgatca gattgttttc gagcagcagc gcttcggcct cggagcgcgt gacggtcgtc
     1201 tcgatgcgcg caatgcgcgt caccatcatc gcgatgcgcg gcgaaagtag cgtcttcgtg
     1261 aagtagctcg agacgcgctt tttcagatcg cgcgccttgc ccacgtaaag caccgtgccc
     1321 tgcgtatcgt aatagcgata gacgccgggc agatgcggca attgggcgag cacttttttc
     1381 ggctcgaaaa cgtcggttgc ttcgggttcg gtcatgcaga atcggttggc tgggacagtg
     1441 tcgcgaaggt ctgcctgcgt gccgcgcgct cgatggtcac tcagcggtca cttaggcgcg
     1501 gaaccgcgga tgagtgcttt agaatcgcca gtttagagca ttccgcgtgc gttcgaaccc
     1561 tgctgcatcg aatggcgtga tgcagcggcg caagcctcta cgcctgcacc tgattgcatc
     1621 acccgccagc aaggtcctgc gcaatccaac cccgcccccg ttccactcta cgacccgccg
     1681 ccatgtccac cgccgcgccc ccttccgaac cggccactgc cgcgcccacg gcaatcgcct
     1741 gcgatatttt ttgcgcggtg atcgataatt tcggcgacat cggcgtgtgc tggcgtctcg
     1801 cgcgccaact ggcgagcgag catggctggc aagtacgcgt gttcgtcgac gacctgcatg
     1861 cgttccagaa actgtgccct tcgctggcgc tggatcgcgc gcgccaggcg gtcggcggca
     1921 tcgtcatcga acactggcac gagccggccc acgcgggcga tacgctcgaa gtggcagacg
     1981 tcgtgatcga ggcttttgcg tgcgagttgc cgtcgatcta cgtcgctgcg atggcgcggc
     2041 gcgaacgcgc gccggtctgg ttcaacctcg aatacctgag cgccgaagac tgggtcgcgg
     2101 atttccatct gcggccctcg ccgcatccac gctacccgtt gaccaagacc ttcttttttc
     2161 ccggactcgg ccccggcacc ggcggcgtgc tgaaggaacg gcatctggac accgcgcggg
     2221 cggccttcga agcctcgcaa cccactcgcg aagcgtggtg gctgaccacc accggccgcg
     2281 cgccgccgcc ctccacggcg acgatcgtct cgctgttcgc ctacgaaaat cccgccgtcg
     2341 acagcctgct cgaacaatgg cgcgacagcg ccgaaccggt cgttctgctg gtaccggagg
     2401 gccgcatttc gggcgcggtc gcgcgctttt tcggtctgtc ttcgttcgcc gccggttcgc
     2461 acgccgagcg tggcggcctc agcgtccacg cgctcgcctt cacggagcaa cccggctacg
     2521 acgcgctgct gtgggccagc gacatcaact tcgtgcgcgg cgaggattcg ttcgtgcgcg
     2581 cccaatgggc cgccaaaccc tttgtctggc acatctaccc gcaggccgac gacgcccatt
     2641 tgcccaagct cgacgccgcg ctcgcccact acggccgcac cttgcccagc aacgcgcggg
     2701 acgcccttgc acgcttctgg cacgcctgga acagcgcggg ccggccggac tgggcggaat
     2761 tccagcacca ccgcgcggcg ctgaaacagc gcgcgaccga ctgggccggc gaactggccc
     2821 aggtcggcga cctcgccgga aatctggcct tgttcgcaaa aagtcagtta aaataagcgg
     2881 ttatccaacg gccgacgacg caagcgcggc ccgatcgcag cgagtaagtc ttgcaaaccc
     2941 ggcttgcaaa ttcgtctggc aaatacgcca gactaactgg ccagaccgat tcgcgaggca
     3001 aacccagcag gccaagccgc ttggccctgg cgcaccccgc atcgaaagat gcatgacccg
     3061 ccctcagctt ggtagtgtgt ggcgcaatgt caaaaaaggg gcgcatcctg cgatcggcgc
     3121 cactcgtgat cacgcccgca cagggtaaaa agcaggtaac ggattgcagg gggttgcagg
     3181 tttgcagcct cgccgcgggt gaccgcggca agccggccat gctccacgca ccgcttccag
     3241 gcggtcccgc gccgtacggc gaggagcagc gaagccgctt gcgccgtatg ccgttgagca
     3301 aaagttgaag ctacttattt cgtacaggac agttttatga agaccgcaca ggaactccgc
     3361 accggcaacg tagtaatgat cggcgcagac gcgatggtcg tgcaaaaggc cgaatacaac
     3421 aaatcgggcc gcaactccgc cgtcgtcaag atgaagttca agaacctgct gaccggcgca
     3481 ggcatggaaa gcgtgtacaa ggcagacgac aaattcgacg tcgtggtgct ggagcgcaag
     3541 gaagtcacct actcgtactt cgctgacccg atgtacgtgt tcatggacgc cgactacaac
     3601 cagttcgaag tcgaaagcga aatgatgggc gacgccctcc attacctcga agacggcatg
     3661 gcttgcgagg tcgtgttcta caacgagaaa gccatttcgg tcgaactgcc gaccacgctg
     3721 gtccgcgaaa tcacctacac ggaaccggct gtcaagggcg atacgtcgtc gggcaaggtg
     3781 ttgaagaacg ccaagctgaa caccggcttc gaattgcaag tgccgctctt ctgcgccatc
     3841 ggcgacaaga tcgaaatcga cacgcgtacg cacgagtatc gcagccgcgc ctaagcgcct
     3901 gccgcctgca tcccacgcct gagcaagtgc ggcgtggcga taaaaaaaat ggcaaaagaa
     3961 aaagcgcccg ctacgggcgc tttttctttt ttgcgccgcc gtgtatggtt gaggaaaact
     4021 ttaccggcaa gcttctcgaa aatgccagtc cgttaccgtt tacttcctgc tccacgttcg
     4081 acaaaccatt gataaattaa agtaattggc tttggcacag tccatgcttc tcttgaggtg
     4141 taggcccgac acggctttat ttcatcaact ccggagatgc gatatgaata acgacatcgc
     4201 tgaaggcaag tggaagcaaa tggttggtaa ggccaaggca gcatggggtg aattgacgga
     4261 cgacgagcta accaaggcgg aaggccacgc cgacagactg gcggggctga tccaggagcg
     4321 ttacggcaag acccg
//
LOCUS       DATASH010000770         1708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2105266,
            whole genome shotgun sequence.
ACCESSION   DATASH010000770 DATASH010000000
VERSION     DATASH010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2105266"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..1530)
                     /gene="cysN"
                     /locus_tag="VHV99_20605"
     CDS             complement(100..1530)
                     /gene="cysN"
                     /locus_tag="VHV99_20605"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF003478.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HEX3637839.1"
                     /translation="MAALPVIDIQKFDVEEFLRQEQSKDLLRFTTAGSVDDGKSTLIG
                     RLLHDARGAYEDQLKQAIRGGEIDFSLLTDGLRAEREQGITIDVAYRYFATPKRKFIL
                     ADTPGHEQYTRNMATGASTAQLAIILLDAKRGITTQSMRHAYIASLLGIPHFVIAVNK
                     MDLVDYDQQVFHRIRAEFAPFLTRLGVDGAYFLPLSALKGDNVVDKSKNMPWFDGHSL
                     LDHLESVDTSPVTVHAPFRMAVQRVVRPDATFRGYAGQIASGVVRPGDEVLAQPSGRR
                     TRVERIVAWEGDLEVAHAPMSVTLTLADEIDISRGDMVSSGPEPIAARAIEATMVWFD
                     SKPLDPAGHYLIKHTSQMVPARVESVEYRVNVNTLHAESVRSLAMNEVGVVHLATARP
                     IFFDPYRENRGAGAFILIDRQTNATAAAGMIISATEAGTESAAERLARLVRASVPPGA
                     HLNLPAEEEAAIALLRKALQGFFRHE"
BASE COUNT          309 a          497 c          549 g          353 t
ORIGIN      
        1 acgcactcgg cctgaaaact ggaagtgatg caagccgcgc cgttcttcgc cagctcgtcc
       61 gtgatcaact gccgggcgtc tcgaattttc tgatccagat tactcatgac ggaagaatcc
      121 ttgcagtgct ttgcgcagca gcgcgatggc ggcttcttct tctgccggta ggtttagatg
      181 cgcgcctggg ggaacggatg cacggaccaa tctcgccagg cgctcggcgg cgctttctgt
      241 gccggcttcc gtggcggaaa tgatcatgcc cgcggccgcc gtggcgttgg tctggcggtc
      301 gatgaggatg aaggcgcctg ctccgcgatt ctcgcgatag ggatcgaaga aaatcgggcg
      361 ggctgtcgcg agatgcacca cgccgacttc gttcatcgcg aggctgcgca cggattcggc
      421 gtgcagcgtg ttcacgttga cgcgatattc gaccgattcg acgcgcgctg gaaccatctg
      481 cgacgtgtgt ttgatcagat aatggccggc gggatcgagc ggcttcgaat cgaaccagac
      541 catggtcgct tcgatcgcgc gcgcggctat cggctccgga ccactgctga ccatgtcgcc
      601 gcggctgatg tcgatctcgt cggcgagtgt gagcgtcacc gacatgggag cgtgcgctac
      661 ttcgagatcg ccttcccagg ccacgatgcg ctcgacgcgg gtgcggcggc cggagggctg
      721 ggctagaact tcgtcacccg gacgcaccac gcccgaagca atttgcccgg cgtagccgcg
      781 gaacgtcgca tcgggacgca cgactcgctg gactgccatg cggaaaggcg cgtgaacggt
      841 gaccggcgag gtgtcaaccg attccagatg atcgagcagg ctgtggccat cgaaccacgg
      901 catgttcttg cttttgtcga caacgttgtc gcccttgagg gcgctgagcg gcaggaagta
      961 ggcgccgtcg acgcccaagc gcgtgaggaa cggagcgaac tcggcgcgaa tccggtggaa
     1021 gacttgctga tcgtaatcga ccaggtccat cttatttact gcgattacga aatgggggat
     1081 gcccagcagc gacgcaatat acgcgtggcg catggattgc gtggtgatgc cgcgcttggc
     1141 gtccagcaga atgatggcga gctgcgcggt cgaagcgccg gtggccatgt tgcgggtgta
     1201 ctgctcatga cccggcgtgt cggccagaat aaatttgcgt ttgggcgtcg cgaagtatcg
     1261 gtaggctacg tcgatggtaa tgccctgctc gcgttcggcg cgcaagccgt cggtcagcag
     1321 tgagaaatcg atttcgccgc cgcggatggc ctgcttcaat tggtcttcgt aggctccgcg
     1381 cgcatcgtgc agcaaacgcc cgatcagcgt ggatttccca tcgtcaacgc tgccggctgt
     1441 cgtgaagcgc agcaggtctt tcgactgctc ctgccgcaga aactcctcaa cgtcgaactt
     1501 ctgaatgtca atcacgggaa gcgccgccat ttttttagaa gtacccttcg cgcttcttta
     1561 attccatcga cccttcgcgg tcgtggtcga tgacccgatt ctcacgctcg gaatttctga
     1621 cggcgactaa ctccgcgatg atcttgggaa tggtgtcggc gtccgaacgg atcgcgccgg
     1681 tgcacggcga gcatcccagc gaacgcat
//
LOCUS       DATASH010000771         7339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1315213,
            whole genome shotgun sequence.
ACCESSION   DATASH010000771 DATASH010000000
VERSION     DATASH010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7339
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1315213"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VHV99_20610"
     CDS             complement(<1..257)
                     /locus_tag="VHV99_20610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX3637840.1"
                     /translation="MNRLSSALTAMQAHYDVVVVGSGYGGAIAASRMARAGKNVCVLE
                     RGREFMAGEYPRTPLEGAAQMQYNTALAQIGSPLALLEVHV"
     gene            complement(510..1301)
                     /locus_tag="VHV99_20615"
     CDS             complement(510..1301)
                     /locus_tag="VHV99_20615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637841.1"
                     /translation="MGAVPSHAFVRSLDDAILPDLWRRRTQLSADEMVSMVELVKRAL
                     RTYHPLELQALGEDKEELVAQFIYSKVLRLAPGHTETHACADSAPSNGYALCAYFRRY
                     LIDCLRSAGHQRNVSMENEGMAQEIDLHAHALEDPVESVLLQYGLDEQRVRFAARAFI
                     DSLDEPERIVLAGSLGWCSEGKGGLSAVAAQHRVPSYHYRAVKLGVTMKKTAGADDFS
                     NTKIGRWLTDVLGIEIDIDNRAAILLVLNLLAAESSDSEMNEVAA"
     gene            1847..3718
                     /locus_tag="VHV99_20620"
     CDS             1847..3718
                     /locus_tag="VHV99_20620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEX3637842.1"
                     /translation="MRADPIQRAIDEDLVRVLYAQDPIAFFSHWFSIAVLVAIYWPNV
                     PSPPLFIACFGFYAVANCCSLALWICNRRYPHLTTPRGWINLHAVRGVLLYSAPGLAV
                     WFAFQRPQTDLPLLHTVMLVTLAAGVFMSNGFDLLNFSTAIPFLLFPTIVLHFGAHTF
                     DRTILAIVLAFFFCAINVYALSYRKLFQQVVQARVDQQYLAESLAAQKRVAEEASLAK
                     TRFFAAASHDLRQPLHAIGLLAASLNDPAATPVQHAKTAQHIVYNVEALNQLFNQVLD
                     LARLESGVTQVIRLHFRLSELFERVGSQYRPQAAAKGLALRIAPASAVVHDDPVLLER
                     VLSNLLSNAVRYTEEGAIWVGYRRAGRRSGGYIEVRDSGIGIPAEEHERIFEEFYQVA
                     NPQRDARQGHGLGLPTVKRLVEMLGGELQMRSAPGRGSVFRFPVQAGDAGGIVASLNE
                     AVAGGPSAQGRRVLCIDDEPSILEGLSSLLGRWGCIVHGARDEADALTALEEGFVPDA
                     VLCDYQLANHRTGAQALTAVRNVLARAGHEGVVTLLITGDMASAELAALALQGIPVLH
                     KPVTPARLRRTLEMLWQQAELDKGRPVPTQEMHAGEAPSVHNAAGTGLEKGALKDAT"
     gene            complement(3815..4573)
                     /locus_tag="VHV99_20625"
     CDS             complement(3815..4573)
                     /locus_tag="VHV99_20625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3637843.1"
                     /translation="MKFLVADDHELIRQGVKGLLRGLDPDAQFDEADSWETLAAAARP
                     DADHDLAIVDLHMPGMSGASSLEVLLKANPALPVVVLSAEESPDEMRAVLAAGALGFV
                     PKRQPASVMLKAIELVLSGGAYVPMEALSLLGARETQAAPAHADTATDLAAQGAAHAS
                     SQAGAALTEPITQIQALQPHQQHLLENLSPRQQDIMRLVHRGWTNKMIARELGVAEGT
                     VKVHLSVIFRALGVHNRSTAIAVINGWLEAGKTL"
     gene            complement(4957..5148)
                     /locus_tag="VHV99_20630"
     CDS             complement(4957..5148)
                     /locus_tag="VHV99_20630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012493604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637844.1"
                     /translation="MTTNITITKDTGSHAPDSASQTPGRKLPGWVWFIALWCFGVASA
                     VSLGFAFKLLMNATLFAVK"
     gene            complement(5123..6127)
                     /gene="cydB"
                     /locus_tag="VHV99_20635"
     CDS             complement(5123..6127)
                     /gene="cydB"
                     /locus_tag="VHV99_20635"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEX3637845.1"
                     /translation="MDVTVVWAAIIALGLFMYVVLDGFDLGIGIIFPFFPDEKERDLM
                     MNTVAPVWDGNETWLVLGGAALFAVFPVVYSTVLSALYLPLIFMLVCLIFRGVSFEIR
                     AKAQRTKQLWDLAFIGGSAGATFFQGIVLGAFLQGIPVNDGVYAGEAFGWLTPFSLLT
                     GLGLVITYALLGCCWLVAKTEGDLQRRLHRVVWPLTLVLLAFIAMVSLWTPLQEPTIA
                     QRWFHDKLFYRLLPVPFLVAICTFFMHRAVRERHHNTPFVLALLLVLLGYAGLLVSLW
                     PYAIPSSMTLWEAAAPRSSQMFTLVGAAVILPIIIAYTTMGYWVFRGKVRHDDQHHYH
                     "
     gene            complement(6151..>7339)
                     /locus_tag="VHV99_20640"
     CDS             complement(6151..>7339)
                     /locus_tag="VHV99_20640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEX3637846.1"
                     /translation="MSYQFGTNWSGFSKFAGPITGPLLMYEVMTAFFLEAGFLGIMLF
                     GWQRVSPRAHFGATLMVATGTLISTFWILASNSWMQTPQGFEVVDGHVVPLDWFKIIF
                     NPSFPYRLAHMALAAFIVAGLVVSAVGAWHLLRGRRDPAVKKMFSMALWLLLILTPIQ
                     AFVGDQHGLNTRKYQPAKIAAIEGLWETEKGGTALNLFGIPDMQAETTRYAVSIPHLG
                     SLILTHSWDGEIRGLKEFPPQDRPDSTVVFWSFRIMAGLGVLMILMSLTAWALRRHER
                     LFEAKWFQRVAVAMGPTGFITLLAGWVTTEAGRQPWVVYGVMRTSQAVSPLTTQQVGI
                     SLMAFVVVYFLVFSTGIYYMLKLMRSGPALPGQTPDGVPEHTPNQTAGRPLSAADHMI
                     DAA"
BASE COUNT         1384 a         2248 c         2347 g         1360 t
ORIGIN      
        1 ttcacgtgca cttcgagcag cgcaagcggt gagccgattt gcgcgagtgc cgtgttgtac
       61 tgcatctggg cggcgccttc gagcggcgtg cgcggatatt cgccggccat gaattcccgt
      121 ccgcgttcga gcacgcacac atttttgccg gcgcgcgcca tccggctcgc ggcaattgca
      181 ccgccataac ccgaacccac taccaccacg tcgtaatgcg cttgcatcgc cgtcagggcg
      241 ctcgataagc ggttcatgga gaccacctcg tcaggaagga actgccgggt aggggaaccc
      301 ggacttcgga aagaagacgg tgtgtggcta aggctttttt tatcgtaggc ggtgcgcgat
      361 gacaaaaaaa ggcgcatcgt acgtggcgcg tgaagcgtga agcgtgaagc gtgaagcgtg
      421 aggcgtgagg cgtgaagcgt ggcgagcggc gcgatttctg aaagggacga agtgccgcgg
      481 ccggcctgcg cgagtctgtc agtgggctat taggcggcta cttcattcat ttcgctgtcg
      541 ctcgattccg cggcgagcag gttcaacacg agcagaatcg ccgcgcgatt gtcgatgtcg
      601 atctcgatgc cgagcacgtc ggtcagccag cggccgatct tggtattgga aaaatcgtcg
      661 gcgccggccg tctttttcat ggtgacgccg agtttgacgg cacgatagtg atacgaaggc
      721 acacgatgct gagcagcgac tgccgacaga ccgccttttc cttccgagca ccagccgagg
      781 ctgccggcca gcacgatgcg ctccggctcg tcgaggctgt cgataaaggc gcgcgccgcg
      841 aagcgcacgc gttgttcatc gagcccgtat tgcagcagca cgctttcgac gggatcttcc
      901 agcgcgtgcg cgtgcagatc gatttcctgc gccatgcctt cgttttccat cgacacgttg
      961 cgctggtgac ccgcgctacg cagacagtcg atcaggtagc ggcgaaaata cgcgcacagt
     1021 gcgtagccat tggacggcgc gctgtcggcg caggcatggg tttccgtatg gcccggcgcg
     1081 aggcgcagta ctttcgaata gatgaactgc gcgaccagtt cttccttgtc ctcgccgagc
     1141 gcctgcaact cgagcgggtg ataggtccgc agcgcccgtt tgaccagctc gaccatcgac
     1201 accatttcgt cggcggacaa ttgggtacgc cgacgccaca ggtcgggcag gatcgcatcg
     1261 tcgagactgc ggacgaacgc gtggctcgga actgcgccca tgaaaacctc cataaggggt
     1321 aagacaagga gcgcgggggg cctcgactgc aggcaaggaa gcggggtgtc tgaaacggtg
     1381 agccgcgctg gaatgtgaag ccagtatggt ttccggcggc gccttcgcct atatggatta
     1441 agtggtagct gatgggtaaa ctgccgggta aaccttccgg gcgtcggcat ggttgtttgt
     1501 ctgtttgtcg tttgaaaacc gtgcgctgca ccaaaaggct gcagcggcgc ggcgcagcat
     1561 cgcaacacct gtgcacgagt gcctgcgcgg catgccaggc cttccgtgcc cgcgtcgctg
     1621 cgcttgcgtt ccacgccatc gaatctgccg tgcccgacag caaccatttt gcgtatcgac
     1681 cgcaaaatgc acgggaccag agtaagtgct ctgcatggga cccgagtaag cgctatagcg
     1741 ccaattctgg tcgacagcta aagcgctgac tctggtcgac cgcagaatgc atgggaccgc
     1801 agtaagtgct aaagcactaa ctgcggtcga caaaggagac cgcatgatgc gggccgaccc
     1861 catccagcgt gcaatcgacg aagatctggt gcgcgtgctg tacgcgcaag atccgatcgc
     1921 cttcttttcg cactggttct cgatcgcggt gctggtggcg atctactggc cgaatgtgcc
     1981 gtcgccgccg ctcttcatcg cgtgcttcgg gttttatgcg gtggccaatt gctgcagcct
     2041 cgcgctgtgg atctgcaatc gccgctaccc gcatctcacg acgccgcgtg gctggatcaa
     2101 cctgcacgcg gtgcgtggcg tgctgctgta cagtgcgcct ggcctcgcgg tgtggttcgc
     2161 gttccagcgc ccgcaaaccg atttgccgct gctgcatacg gtgatgctcg tcacgctggc
     2221 ggccggcgtg ttcatgtcga acggcttcga cttgctgaac ttttcgaccg cgatcccgtt
     2281 tctgctgttt ccgactatcg tgctgcattt cggcgcgcat acgttcgacc gtacgatcct
     2341 cgcgatcgtc ctcgcgtttt tcttctgcgc gatcaacgtt tatgcgctga gctatcgcaa
     2401 gctgttccag caggtcgtgc aggcgcgtgt cgatcaacag tacctggcgg agtcgctggc
     2461 ggcgcaaaag cgtgtggccg aagaggccag tctcgctaaa acgcgctttt ttgcggccgc
     2521 gagccacgat ttgcgccagc cgctgcatgc gattggcctg ctggcggctt cgctcaacga
     2581 cccggcggcg acgcccgtgc agcatgcgaa gacggcccag cacatcgtct acaacgtcga
     2641 ggcgttgaac cagttgttca accaggtgct cgatctcgcc aggctcgaaa gcggcgtgac
     2701 ccaggtgatc cgcttgcatt tccggctttc ggaactgttc gagcgggtcg gcagccagta
     2761 ccgtccgcaa gccgcggcca agggcctggc tttgcggatc gcgccggcct cggcggtcgt
     2821 gcatgacgat ccggtgctgc tcgaacgcgt gctgagcaat ctgctttcga atgcggtgcg
     2881 ctataccgag gaaggcgcga tctgggtcgg ctatcggcgc gccgggcgtc gttcgggtgg
     2941 gtatatcgag gtgcgcgatt ccggcatcgg cattccggct gaggaacatg agcgcatctt
     3001 cgaagagttc tatcaggtcg ccaatccgca gcgcgacgca cgccagggcc acggtttggg
     3061 cttgcctacg gtgaagcgcc tcgtcgagat gctcggcggt gagttgcaga tgcgctcggc
     3121 gccgggccgc ggctcggtgt tccgctttcc ggtgcaggcg ggcgacgccg gcgggattgt
     3181 cgccagcctg aacgaggcgg tggcgggcgg gccgtcggcg caagggcggc gcgtcctgtg
     3241 catcgacgac gaaccgtcga ttctcgaagg cctcagcagt ctgctgggac gctggggttg
     3301 catcgtgcat ggcgcacgcg acgaggccga cgcgctcacg gcgctggagg agggtttcgt
     3361 gccggacgcc gtgttatgcg actaccagtt ggcgaatcac cgaaccggcg cgcaggcgct
     3421 caccgccgtg cgcaatgtgc tcgcaagggc ggggcatgaa ggtgtggtga cgttgctgat
     3481 tacgggcgat atggcatcgg cggaactggc ggcgctggcc ttgcagggaa ttccggtgct
     3541 gcataagccg gtcacgcctg cgcgtttgcg gcgcaccctg gaaatgctgt ggcagcaggc
     3601 ggaactggat aaagggcgcc cggtgcccac gcaggaaatg catgcaggcg aagcgccgag
     3661 cgtgcacaac gcagcgggaa ccgggctgga gaaaggagcg ctgaaggacg caacgtgaga
     3721 agcggaacgc cgtcgctcac cgcggcaagt gctcgatcgc agatcaatcc caatgcgagc
     3781 tgtaagtaga agcagcctgc cgcccacccc ggtattacag ggttttcccg gcctcgagcc
     3841 agccgttgat cacggcgatc gcagtggatc ggttgtgcac gccgagcgcc cggaagatca
     3901 ccgacagatg caccttaacc gtcccttccg cgacgcccag ctcgcgcgcg atcatcttgt
     3961 tggtccagcc acgatgcacc agccgcatga tgtcctgctg tcttggcgac aggttctcca
     4021 gcaaatgttg ctggtgcggt tgcagcgcct ggatttgcgt gatcggttcg gtgagcgcgg
     4081 cgccggcctg actggaggcg tgtgccgcgc cctgagcggc aaggtcggtg gccgtgtcgg
     4141 catgcgccgg tgcggcctgc gtctcacgcg cgcccagcag gctgagcgct tccatcggca
     4201 cataggctcc gcctgacaac accagttcga ttgctttgag catgacgctg gcgggttgcc
     4261 gcttgggcac gaagccgagc gcgccggcgg ccagcaccgc gcgcatttcg tccggcgatt
     4321 cttcggcgga aagcacgacg accggcaacg cgggattcgc cttcaacagc acttcaagcg
     4381 acgacgcgcc gctcatgccc ggcatatgca gatcgacgat cgcaaggtcg tgatcggcat
     4441 cgggccgcgc ggcggcggcg agtgtttccc agctatcggc ttcgtcgaat tgtgcgtcgg
     4501 gatcgaggcc gcgcagcagc cccttgacgc cttgccggat cagttcatgg tcgtcggcca
     4561 caagaaactt catgatgtct ccgcgagtcg ggcctgcgcg tggtggacta ccgccgctgt
     4621 gccgctggcc ttaaagccag tggccctaca gcttgtggct ttacttgccg ttttacttct
     4681 attttcgaat cgcgtcattg ccggctgccc cgcgtttcat atgccgatcg atggcgcggc
     4741 gcgccttccg gtttgccgca tattcgacac gctattgatg tgatcgccgg ccggttcgat
     4801 gcaccagcat ggtgtgtaag gcaggtctcc cgcacatagg ttccggcttg ctacgaagac
     4861 gagccgcacg ccccaatttt taagccgtcc acgcaggaat ccaaaggtta tgcgcaccgg
     4921 atgagatcac gtaaaaccag tattcatttg accgcttcat ttgactgcga acaacgtcgc
     4981 attcatcagc agtttgaacg cgaagccgag cgacaccgcc gaggccacgc cgaagcacca
     5041 gagcgcgatg aaccacaccc agccgggcag tttgcggccc ggcgtttggg aagcgctgtc
     5101 cggggcgtgg ctgcccgtgt ccttagtgat agtgatgttg gtcgtcatga cgcaccttgc
     5161 cacgaaatac ccagtaaccc atcgtcgtat aggcgatgat gatcggcaga atcaccgctg
     5221 cgccaaccag cgtgaacatc tggctcgagc gcggcgcggc ggcttcccag agcgtcatgc
     5281 tcgacggaat cgcatacggc cacaggctca ccagcagccc ggcatagccg agcagcacca
     5341 gcagcagcgc cagcacgaac ggcgtattgt ggtgccgctc acgcaccgcg cggtgcatga
     5401 agaacgtgca gatcgccacg aggaacggca ccggcagcag acggtagaac agtttgtcat
     5461 ggaaccagcg ttgcgcgata gtcggttctt gcagcggcgt ccacaggctg accatcgcga
     5521 tgaaggcgag tagcacaagc gtcaacggcc acaccacgcg atgcaggcgg cgttgcagat
     5581 cgccttcggt cttcgcgacc agccagcagc agccgagcag cgcgtaggtg atcaccagac
     5641 cgagacccgt cagcagactg aacggcgtga gccagccgaa ggcttcgccc gcatagacgc
     5701 cgtcgttcac cggaatgcct tgcaggaacg cgcccagcac gatgccctgg aagaaggtcg
     5761 cgcccgccga gccgccgatg aacgccagat cccacagctg tttcgtgcgc tgggccttgg
     5821 cgcgaatttc aaacgatacg ccgcgaaaga tcaggcatac gagcatgaag atcagcggca
     5881 gatacagcgc cgacagcaca gtcgaataga ccaccggaaa caccgcgaac agcgcggcgc
     5941 cgccgagcac cagccaggtt tcgttgccgt cccacacggg cgcgacggtg ttcatcatca
     6001 ggtcgcgttc cttctcatcg gggaagaacg ggaagatgat gccgatgccc agatcgaagc
     6061 cgtccagcac cacgtacatg aataggccca gcgcgatgat cgcggcccac actacggtta
     6121 cgtccatttt ctttctcgca aacagtcagg ttaggcggcg tcgatcatgt gatcggcggc
     6181 ggacagcggg cggcccgcgg tctgattggg cgtgtgctcg ggcacaccgt ccggcgtctg
     6241 tcccggcagt gccggacccg agcgcatcag cttgagcatg tagtagatgc cggtactgaa
     6301 cacgaggaag tagacgacca cgaacgccat cagcgagatg ccgacctgct gcgtggtgag
     6361 cggtgatacc gcctgcgagg tgcgcatcac gccgtacacc acccatggct gacggcctgc
     6421 ttcagtggtg acccaacccg ccagcagcgt gatgaagccg gtcggaccca ttgccacggc
     6481 gacgcgttga aaccactttg cttcgaacag ccgttcgtgg cggcgcagcg cccatgcggt
     6541 gagagacatc aggatcatca gcacgccgag cccggccatg atgcgaaagc tccagaacac
     6601 caccgtcgag tcgggccggt cttgcggcgg gaattccttc aggccgcgaa tctcgccgtc
     6661 ccagctatgc gtgaggatca ggctgccgag gtgcggaatc gacacggcat aacgcgtggt
     6721 ctccgcttgc atatcaggaa tgccaaacag attcagtgcc gtgccgcctt tctcggtttc
     6781 ccacagacct tcgatcgcgg cgatcttggc cggctgatac ttgcgcgtgt tcaaaccatg
     6841 ctgatcgccg acgaatgcct ggatcggcgt gaggatcagc agcagccaga gtgccatcga
     6901 gaacatcttc ttgacggccg gatcgcgccg tccgcgcagc agatgccatg cgcccaccgc
     6961 cgacaccacc agccccgcga cgatgaaggc cgccagtgcc atatgcgcga gccggtacgg
     7021 aaacgacgga ttgaaaatga tcttgaacca gtcgagcggt acgacatggc catcgacgac
     7081 ttcaaagcct tgcggcgttt gcatccagct attggacgcg aggatccaga aggtcgagat
     7141 cagcgtgccg gtcgccacca tcagcgtcgc gccgaagtgg gcgcgcggac tcacgcgctg
     7201 ccagccgaac agcatgatgc cgaggaagcc cgcttcgagg aagaacgccg tcatgacctc
     7261 gtacatcagc agcggtccgg tgatcggccc ggcgaatttg gagaagcccg accagttggt
     7321 gccgaactga taactcatc
//
LOCUS       DATASH010000772         3150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1315227,
            whole genome shotgun sequence.
ACCESSION   DATASH010000772 DATASH010000000
VERSION     DATASH010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1315227"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VHV99_20645"
     CDS             <1..594
                     /locus_tag="VHV99_20645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX3637847.1"
                     /translation="PAGLVEAAFDELARRWRPILDAFDQAGVDVCYELHPGEDLHDGV
                     TFERFLHALKQHPRANILYDPSHFVLQQLDYLAFIDIYHERIKAFHVKDAEFRPNGKQ
                     GVYGGYSGWVERAGRFRSLGDGQIDFGAIFSKMAQYDFPGWAVLEWECALKHPQDGAR
                     EGAEFIRRHIIRVADHAFDDFAGSGVDAAQLKRVLGI"
     gene            678..1823
                     /locus_tag="VHV99_20650"
     CDS             678..1823
                     /locus_tag="VHV99_20650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3637848.1"
                     /translation="MPQRRLRLGMVGGGQGAFIGAVHRIAARLDDRFELVAGALSSDP
                     QRAQASAAEAGIARSYTDWREMARVEAARGDGIDAVAIVTPNHLHAPVATAFLEAGIH
                     VVCDKPLAISLAEGEALAKLAREKNKLFALTHTYSGYPLVRHARELVEKGELGEIRVV
                     QVEYAQDWLAEPIETSGTNKQAGWRTDPSLAGPAGCLGDIGTHAYHLAAFITGMTPSS
                     LSAELHTFVPGRRIDDHVQAMLRYSNGARGMLWASQVASGAENALRLRVYGTKAGLSF
                     DQENPNELWFTPLGGSAERLTRGRVKSAIAAHGTRVPAGHPEGYLEAFAQLYKDAALQ
                     IEALDAGRALPPESLLLTTVDDGVAGLRFIDAVLASSAADGQWREIY"
     gene            1857..2147
                     /locus_tag="VHV99_20655"
     CDS             1857..2147
                     /locus_tag="VHV99_20655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637849.1"
                     /translation="MLSLEVAANIDAGDFFRSFISRQQLVNVLAVKSRHKGFPLAQTG
                     ASFFNSLTRALNAFCPFDRADLLWSGAMLTDLAPVLVMGLDLFSRQIGCNGA"
     gene            complement(2144..2404)
                     /locus_tag="VHV99_20660"
     CDS             complement(2144..2404)
                     /locus_tag="VHV99_20660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637850.1"
                     /translation="MRTTRAIHAVERLKTRSGNPQFAAISLSGGLFYLVDKSSGTDKK
                     VSDPLTLDDFVKFVDGFGPPKPRKASKLDIAFEEQIRKSKGS"
     gene            complement(2559..>3150)
                     /locus_tag="VHV99_20665"
     CDS             complement(2559..>3150)
                     /locus_tag="VHV99_20665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3637851.1"
                     /translation="ATERYNRTDASVTGQILKLMAAKPDAILIAGAGTPTVLPQRTLI
                     ERGFKGPIYQTHGIATPEFIKLGGKDVEGTLFPTQPVVVARTLPADHPAKKAALAFTN
                     EYEAKYGPGSVTQVAGDAAGVYPRLQDAVARALKTAQPGTPAFRAALRDELEHAHELV
                     VPNGVVNTSPKDHVGLDQRASVMGIIKNGKFVYLSQ"
BASE COUNT          547 a          900 c         1068 g          635 t
ORIGIN      
        1 ccggcgggtt tggtagaagc cgccttcgac gaactcgcgc gccgctggag gccgattctc
       61 gatgcgttcg accaggccgg tgtcgacgtg tgctacgaac tgcatccggg cgaggacctg
      121 cacgacggcg tgaccttcga gcgctttcta catgcgctca agcagcatcc gcgcgcgaat
      181 atcctctacg acccgagcca tttcgtcttg cagcaactcg actacctcgc gttcatcgac
      241 atctatcacg agcgcatcaa ggcatttcat gtgaaggacg cggagtttcg gccgaacggc
      301 aagcagggtg tgtatggcgg ctatagcggc tgggtcgaac gggcggggcg tttccgatcg
      361 cttggtgacg ggcagatcga tttcggcgcg atcttctcga agatggcgca atacgatttt
      421 cccggctggg cggtgctgga atgggagtgt gcgctgaagc atccgcagga cggcgcgcgt
      481 gaaggcgcgg agtttattcg ccggcacatc attcgggtgg ccgaccatgc gttcgacgac
      541 ttcgcgggca gtggtgtgga tgcggcgcag ttaaagcgcg tgcttggcat ttgaccacgg
      601 gctgaacctg agctggcggc gggttgccat gaagtgactg tgacgcagat gaagcaggcc
      661 acaaaggagt gagggcaatg ccacaaagaa gactcaggct cggcatggtc ggcggcgggc
      721 agggcgcgtt tatcggcgcg gtgcaccgga tcgcggcgcg gctcgacgat cggttcgaac
      781 tggtagcggg cgcgttgtcg tccgatccgc agcgcgcgca agcgagcgcc gccgaggccg
      841 gtatcgcgcg cagctataca gactggcgcg agatggcgcg cgttgaagcg gctcgcggcg
      901 acggtatcga cgcggttgcg atcgtgacgc cgaatcatct gcatgcgccg gtggcgacgg
      961 cttttcttga agcgggtatc catgtggtgt gcgacaagcc gctggcgatt tcgttggcgg
     1021 aaggcgaagc gctggcgaag ctcgctcgcg agaagaacaa gctgttcgcg ttgacgcata
     1081 cgtattccgg ctatccgttg gtgcgccatg ctcgtgagct tgtggagaaa ggtgagcttg
     1141 gcgaaatacg cgtggtgcag gtcgaatacg cgcaagactg gctcgcggag ccgatagaaa
     1201 cgagcggcac gaacaagcag gcagggtggc gcacggatcc ttctttggca ggtccggccg
     1261 gctgtctcgg cgatatcggc acgcatgcgt atcacctggc tgccttcatc acggggatga
     1321 cgccgtcgtc gctatcggcg gagttgcata cgttcgtgcc gggacgccgt atcgacgatc
     1381 acgtgcaggc gatgctgcgc tattcgaatg gtgcacgcgg catgctgtgg gcgagccagg
     1441 tggcgagcgg cgcggagaat gctttacgtc tgcgggtgta tggcacgaag gcgggcttga
     1501 gcttcgatca ggagaatccc aacgaattgt ggttcacgcc gttaggtggt tcggctgagc
     1561 gcctgacgcg aggccgagtt aaaagcgcga tcgccgcgca tgggacgcgc gtgccggcgg
     1621 gacatcctga aggttatctg gaagcgttcg cgcagttgta caaagatgcg gcgttgcaga
     1681 tcgaggcgtt ggatgcgggg cgcgcattgc ctcccgaaag cttgctgctc acaacggtgg
     1741 acgacggtgt ggccggcctg cgcttcattg atgccgtatt ggcaagcagc gcggcggacg
     1801 ggcagtggcg cgagatttat tgaagtcgcc gatgcagaca gatcgcaacc gtccccatgc
     1861 tctcacttga agtagcggct aacatcgatg cgggcgattt ttttcgcagc ttcatttcgc
     1921 ggcagcaact ggtcaacgta cttgcggtga aaagccggca taaaggcttc ccactcgctc
     1981 aaactggcgc gtcgtttttc aattcgctga cgagggcgtt gaacgctttc tgcccctttg
     2041 atagagcaga cttgttgtgg tccggtgcga tgctgacgga tttggcgcct gtcctggtca
     2101 tggggttgga tctgttcagt cgtcaaattg gatgcaatgg agcctagctg cctttgctct
     2161 tcctgatctg ctcttcaaac gcaatatcga gcttgcttgc cttgcgcggc ttgggcggtc
     2221 caaaaccgtc gacgaacttg acgaagtcgt cgagcgtcag cggatcggag actttcttgt
     2281 ctgtgccgct cgatttgtcg accagataaa acagcccgcc ggacaggctg atcgccgcga
     2341 actgcggatt gccgctgcgg gttttcagcc gctcgacggc atggatagct cgggtggtgc
     2401 gcatgatgtg ggaaacggcg aattgaaggt atgcacttta gcagcaattc gccgtgccgc
     2461 ctggccggcg gagagtaatg ggccgtggcg gcgcaccatg caccattcgc catgcgcgcg
     2521 gatcgcgcgg cgctacaccc gccgcgcgcc ggccgtactc actggctcaa ataaacaaac
     2581 ttgccgttct tgatgatgcc catcacgctc gcgcgctgat ccaggccgac gtggtccttc
     2641 gggctcgtat tgaccacacc gttcggcacg accagttcat gcgcgtgctc cagctcgtcg
     2701 cgcagcgcgg cgcggaacgc cggcgtgccc ggctgtgccg tcttcaatgc gcgcgcgacc
     2761 gcatcctgca aacgcggata cacccccgcc gcatcgcccg cgacttgggt cacgctgccc
     2821 gggccgtact tcgcctcgta ctcgttggtg aacgcgagcg ccgctttctt cgccggatga
     2881 tcggccggca gcgtgcgcgc gactacgacc ggctgggtcg ggaacagcgt gccttccacg
     2941 tccttgccgc ccagcttgat gaactcgggc gtggcgatgc cgtgcgtctg atagatcggg
     3001 cctttgaagc cgcgctcgat cagcgtgcgc tgcggcagca cggtcggcgt gccggcgccg
     3061 gcgatcagga tcgcgtccgg cttggcggcc atcagcttga gaatctggcc ggtgacgctg
     3121 gcatcggtgc ggttatagcg ctccgtcgcc
//
LOCUS       DATASH010000773         4803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2895264,
            whole genome shotgun sequence.
ACCESSION   DATASH010000773 DATASH010000000
VERSION     DATASH010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2895264"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1367
                     /locus_tag="VHV99_20670"
     CDS             <1..1367
                     /locus_tag="VHV99_20670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX3637852.1"
                     /translation="DGLKRGLKNRHIQLIALGGAIGTGLFLGSASVLQAAGPSMILGY
                     AIGGIIAFMIMRQLGEMVAQEPVAGSFSHFAYRYWGDFPGFLSGWNYWVLYVLVSMAE
                     LTAVGTYVHYWWPGVPTWVSALVCFAIINAINLANVKAYGETEFWFAIVKVVAVIGMI
                     LFGGYLLISGHGGPQASITNLWASGGFFPHGFHGLFMMLAVIMFSFGGLELIGITAAE
                     ADDPQKSIPKAVNQVIYRILIFYICSLAVLLSLYPWNQVAAGGSPFVMIFSQIGSTLT
                     ANVLNVVVLTAALSVYNSGVYANSRMLYGLAEQGNAPRALMKVDRRGVPYMAIGLSAL
                     ATFTCVIINYLIPAEALGLLMALVVAALVLNWALISLTHLKSRKAMEAAGETLVFKSF
                     WFPVSNWICLAFMALILVILAMTPGLSVSVWLVPAWLVVMWAGYVFKRRRAAIHGGAR
                     MAGR"
     gene            complement(1443..1886)
                     /locus_tag="VHV99_20675"
     CDS             complement(1443..1886)
                     /locus_tag="VHV99_20675"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX3637853.1"
                     /translation="MAVIGGIHVFASSAFPLQWHVLLAQAGAVSIGVGLPFVKTAFTR
                     IVIDTEQITWTQGILHRRTSRLKLSRIQSITSIHPWWLRVFGVGTIILTTNDLAHPVR
                     RLPGIRHANQLCKKLDEAIAMQQERFTAGNTYDPHAPDHAQAHSI"
     gene            complement(2080..2164)
                     /locus_tag="VHV99_20680"
     tRNA            complement(2080..2164)
                     /locus_tag="VHV99_20680"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2128..2130),aa:Leu,seq:caa)
     gene            complement(2284..3669)
                     /gene="hemN"
                     /locus_tag="VHV99_20685"
     CDS             complement(2284..3669)
                     /gene="hemN"
                     /locus_tag="VHV99_20685"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEX3637854.1"
                     /translation="MTTANTLFQPDLLAKYSLNRPRYTSYPTALQFRDTFDPADYARA
                     AADPGASATDLSLYFHIPFCDTVCFYCGCNKVVTKNRAHARPYVAQLKREMALQAACF
                     DTRRPVSQLHWGGGTPTFLSHGEMAELMAAAREHFTLLPDTEGEYSIEVDPREASPET
                     IAHLRTLGFNRLSLGVQDFDPVVQRAINRIQPLEMTASVMQAARDTDFHSVGVDLIYG
                     LPHQTVDSFSRTLDTMIALAPDRLSVFAYAHMPQLFKMQRQMDAATLPSPAARLAILQ
                     RVVERLTGAGYVYIGMDHFALPTDELARAQAQRTLQRNFQGYSTRAECDLIGFGASSI
                     GKVGDVYAQNVKDLPGYAAAIDAGKLAITRGVRLTADDRLRRDVITQLMCNLELRFDE
                     FEAAYGIRFAATFEPELERLRGFEQDGLVSVSGSKLEVRMAGRMLVRNIAMVFDRYLG
                     QQTLERFSRTV"
     gene            3892..4221
                     /locus_tag="VHV99_20690"
     CDS             3892..4221
                     /locus_tag="VHV99_20690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEX3637855.1"
                     /translation="MSDVPNSVVKRESPFNVTDHACRPDCGACCIAPSISSPIPGMPN
                     GKPAGVRCVQLGDDLRCAIFGQTERPACCSGLQPQADMCGTNRGEALAWLTQLELQTQ
                     PQPAARR"
     gene            4316..>4803
                     /locus_tag="VHV99_20695"
     CDS             4316..>4803
                     /locus_tag="VHV99_20695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1439 domain-containing protein"
                     /protein_id="HEX3637856.1"
                     /translation="MTRPAAPTRRRLLLAALASCTALGITVSLAACATPTFPFIPSHY
                     TFSQQQVQDAVQRKFPYQRTVSQVFEVALTNPVVGFLPDANRVSVKLDARFASPFMPQ
                     PVDGVFTLSSELAYDAASKSVVLKSPNVDNVSVSGQAQAYTQQINAAAALLATQLLTN
                     YP"
BASE COUNT          890 a         1412 c         1532 g          969 t
ORIGIN      
        1 aagacggcct gaagcgcggg ctcaagaatc gccatatcca gctgatcgcg ctgggcggcg
       61 cgatcggcac cggcttgttt ctcggctccg ccagcgtgtt gcaggcagcc ggcccgtcga
      121 tgattctcgg ctacgcgatc ggcggcatca tcgccttcat gatcatgcgg cagttgggtg
      181 aaatggtggc gcaggaaccg gtcgccggtt cgttcagcca tttcgcgtac aggtactggg
      241 gcgacttccc cggctttctg tccggctgga actactgggt gctgtacgtg ctcgtgagca
      301 tggccgagct gaccgcggta ggcacctacg tgcattactg gtggccgggg gtgcccacat
      361 gggtctcggc gctggtgtgc ttcgccatca tcaacgcaat caatctcgcc aacgtcaaag
      421 cctatggcga aaccgaattc tggttcgcaa tcgtcaaggt ggtggcggtg atcggcatga
      481 ttctgttcgg cggctatctg ctgatcagcg gtcacggcgg cccgcaagct tcgatcacta
      541 atctctgggc gagcggcggc tttttcccgc atgggtttca cggcctgttc atgatgctcg
      601 cggtcatcat gttctcgttc ggcgggctcg agttgatcgg catcacggcg gccgaggccg
      661 acgatccgca aaagagcatt ccgaaggccg tgaatcaggt gatctaccgg attctgattt
      721 tctacatctg ctcgctggcg gtgctgcttt cgttgtatcc gtggaatcag gtagcggccg
      781 gcggcagccc gttcgtgatg atcttctcgc agatcggctc gacgctgacc gcgaacgtgc
      841 tcaacgtggt ggttctgacg gcggcgctgt cggtgtacaa cagcggcgtg tatgcgaata
      901 gccgcatgct ctacggtctc gcggaacagg gcaatgcgcc gcgcgcgctg atgaaggttg
      961 accggcgcgg tgtgccgtat atggcgatcg gtttgtcggc gctcgccacg tttacctgcg
     1021 tgatcatcaa ctatctgatt ccggccgaag cgctcggttt gctgatggca ctcgtggtgg
     1081 ccgcgctggt gctgaactgg gcgctgatca gcctgaccca tctgaaatcg cgcaaggcga
     1141 tggaagcggc gggcgagacg ctcgtgttca agtcgttctg gttcccggtc agcaactgga
     1201 tctgcctcgc cttcatggcg ctgatcctcg tgattctcgc gatgacgccg ggtctgtcgg
     1261 tctcggtgtg gctcgtgccg gcgtggctgg tcgtgatgtg ggccggctat gtattcaaac
     1321 gccggcgcgc ggcaatacat ggcggcgcgc ggatggcggg gcggtagagt cagaaatctt
     1381 gcagccggcg cttgcgatgc agcggcggcc ggcaccttaa attgatgaac ggagccggct
     1441 gttcaaatgc tgtgcgcctg agcgtgatcg ggggcgtgcg gatcataggt gttgccggca
     1501 gtaaaccgtt cctgttgcat ggctatcgcc tcgtcgagct tcttgcagag ttgattcgcg
     1561 tgcctgatgc ccggcaaacg cctcacggga tgcgccagat cgttggtcgt caaaatgatc
     1621 gtgccgacac cgaatacgcg cagccaccaa gggtggatcg acgtgatgct ctgaatccgg
     1681 gagagtttca gcctcgacgt ccggcggtgc agaatacctt gcgtccaggt aatctgctcg
     1741 gtatcgatga cgatccgggt gaacgctgtc ttgacgaacg gcaagccgac gccgatcgac
     1801 acagcgcctg cttgtgcaag cagcacatgc cattgaagcg ggaaagcgct cgacgcgaag
     1861 acatgtatgc cgccaatcac agccatgacg gcacatcctt tcaggatcgc tggaaggttg
     1921 cagatctgcg acggcgcgcc gctgaaaatc acctgattgg ccatgactga caatagatga
     1981 tctcggctcc cgtgggaagc aaaaaacccg caaggctttt agcttattgc gggctcagtg
     2041 gaaattgagg aattcaccgg tactggccgg aatgctacat ggtgcccagg gccggaatcg
     2101 aaccggcacg ccttgcggcg ggggattttg agtcccctgc gtctaccaat ttcaccacct
     2161 gggcaggtct tgcagcgcac gcgtcagcgt atggcgcgtg gcgaagacgc agattatggc
     2221 cgaaaattcg acgacgggca agctggtcgc tttctttgcg ttcatcgtcc tcatttagcg
     2281 ttttcaaacg gtgcgggaaa accgttcgag cgtctgctga ccgagatagc ggtcgaacac
     2341 catcgcgatg ttgcgcacga gcatgcgccc ggccatccgc acttcgagct tgctgccgct
     2401 caccgacacc aggccgtcct gctcgaagcc gcgcaagcgc tccagttccg gctcgaacgt
     2461 agcggcgaag cggatgccat aagcggcttc gaattcgtcg aagcgcagtt cgagattgca
     2521 catcaattgc gtgatcacgt cgcgccgcaa acggtcgtcc gctgtaagcc gcacgccacg
     2581 cgtgatcgcg agcttgcctg cgtcgatcgc cgcggcgtag ccgggcagat ccttgacgtt
     2641 ttgcgcatat acatcgccga ccttgccgat cgacgacgcg ccgaagccga tcaaatcgca
     2701 ttccgcgcgc gtgctatagc cctggaaatt gcgctgcaac gtacgctgcg cttgcgcgcg
     2761 tgcgagctcg tcggtgggca gcgcgaaatg atccatgccg atgtagacat agcccgcgcc
     2821 cgtcagccgc tcgactaccc gttgcaggat cgcgagccgc gcggcaggcg agggcagcgt
     2881 ggccgcgtcc atctgacgtt gcatcttgaa gagttgcggc atgtgcgcgt aggcaaacac
     2941 cgaaagacga tcgggcgcga gtgcgatcat cgtgtcgagc gtgcggctga aactatcgac
     3001 tgtttgatgc ggcagcccgt aaatcagatc gacgccgacc gaatgaaagt ccgtatcgcg
     3061 cgcggcttgc atcaccgacg cggtcatctc gagcggttga atgcgattga tcgcccgctg
     3121 taccaccgga tcgaaatcct gcacgccgag actgaggcgg ttgaagccga gcgtacgcag
     3181 atgcgcaatc gtttcgggcg atgcttcgcg cggatcgact tcgatcgaat attcgccttc
     3241 ggtgtcgggc agcaacgtga agtgctcgcg cgccgccgcc atcagttccg ccatttcgcc
     3301 gtgcgacagg aacgtgggcg tgccgccgcc ccaatgcaat tgcgacacgg ggcgccgcgt
     3361 gtcgaaacac gctgcctgca aagccatttc gcgtttcagt tgagcgacgt aaggacgcgc
     3421 atgcgcacgg tttttcgtga cgaccttgtt gcaaccgcag tagaagcaca cggtgtcaca
     3481 aaacggaatg tggaagtaca gcgagagatc ggtggccgag gcgcctggat cggcggcggc
     3541 gcgcgcatag tcagcgggat cgaacgtgtc gcggaactgc aaggcggtcg ggtaggacgt
     3601 ataccgtgga cggttcaagc tgtatttagc gaggaggtcg ggttggaata gcgtgttggc
     3661 ggtggtcatg gggcgctcag gtctgacggc caccgtggaa gaggcaagag ccggcggcgc
     3721 ttcggaacac cggatcgaac agcgcaaacg cctcttgagc gtggcctttc gtatccaccg
     3781 agtttacgtg agcgactttc tgccggattg tgtaaaaagg tcgcacggga tgcgatggca
     3841 caatgcgggt ctggtatgac gttgtatgtt ttcgagttca ggaaaaatgt cgtgagtgat
     3901 gtgccgaaca gtgtggtgaa gcgcgagtcc ccgttcaatg tgaccgatca tgcgtgccgt
     3961 cccgattgcg gcgcgtgttg tatcgcgccg tcgatttcga gcccgattcc gggcatgccg
     4021 aatggcaagc cggcgggcgt gcgctgcgtg caactcggcg acgatctgcg ctgcgcgatc
     4081 ttcggtcaga cggagcggcc ggcgtgctgt tccggtttgc agccgcaagc ggatatgtgt
     4141 ggcacgaacc gcggtgaggc actcgcgtgg ctgacccagc tcgaattgca gactcaaccg
     4201 caaccagcgg cgcgccgcta gcacacgcgg gcgaaccgtc acgtcactca ttgattcgaa
     4261 tcagaacaac ctcacgcaac cgcatgctcg agaaggcttc cacaggagat ttcgcatgac
     4321 tcgacccgct gcgccgaccc ggcgccgctt actgctcgcc gcgctggcaa gctgcaccgc
     4381 gctcggcatc accgtgtcgc tcgcggcctg cgcgacgccg acctttccgt tcatcccttc
     4441 gcactacacg ttctcgcagc aacaggttca ggacgcggtg cagcgtaaat tcccctatca
     4501 gcgcaccgtc tcgcaagtgt tcgaggtcgc gctgaccaat ccggtggtcg gcttcctgcc
     4561 ggatgcaaac cgcgtctcgg tgaagctcga cgcccgcttc gcgagcccgt tcatgccgca
     4621 accggtcgac ggcgtattca cgctgtcgag cgaattggcc tacgacgccg ccagcaaatc
     4681 ggtcgtgctc aagtcgccga acgtcgacaa cgtcagcgta agcgggcagg cgcaggccta
     4741 cacgcagcag atcaacgccg cggcggcact gctcgccacg cagttgctga ccaactatcc
     4801 gat
//
LOCUS       DATASH010000774         7042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2105810,
            whole genome shotgun sequence.
ACCESSION   DATASH010000774 DATASH010000000
VERSION     DATASH010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7042
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2105810"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="VHV99_20700"
     CDS             complement(<1..719)
                     /locus_tag="VHV99_20700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX3637857.1"
                     /translation="MKAPSVYLHALGMINALGGDLDTIVPALAAGQAPGMANAHTGIG
                     EAFVGSVLTPLDLAPRAGLARYDCRNNRLLLAALTQIEPAIIAARERYGAHRIGVVLG
                     TSTSGIEAAEAAFVYHAQAGKLPANFSYRQMEIGTAAPFAAAALGVHGPAFTISTACT
                     SSAKAFASARRLLQLRLCDAVVVGGVDSLCELTVQGFASLESTSNVRSNPMSRNRCGI
                     NVGEGAAVFLMSREEAAVRLAG"
     gene            complement(716..3316)
                     /locus_tag="VHV99_20705"
     CDS             complement(716..3316)
                     /locus_tag="VHV99_20705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX3637858.1"
                     /translation="MQVLQQRSAKQAWGVRAAWLLLALAAALYCGWRFAGPSPLQTNL
                     LALLPATEADPVAEKAVDTLASALGDRTVFLVTSNDDAHAKAAAKQLGASLQKSGAFG
                     SVTAELPPFDLSQIAALYMPYRFGLLAPADRAALAQSNASSPTLHGASQEAARPEGSP
                     QGGLRDALAQRIYSPLRGGLTTPLADDPFGWLEHWLGGLPLATSDLEVEDNMLVSHRG
                     TATSVLIIATLPGSAYESKTQRAVLAALAQGESALKQAFPDVSVARTGAVFYAESARS
                     ASEHEVHLIGVASLCGIALLMMWVFRSPRLLLLGFVSTALGIVCALAVTMLVFGQLHL
                     LTLVFGASLIGEAVDYSIQYFVVYLGAGRDWDSHRGARAVRPALTVALATSLLGYAIL
                     TWVPFPALKQIACFAIVGIVTAFASVLSLLPALLTRAPKRSPQRVFAGAARVLTVWHR
                     MIGGKRAWFVAALLLIVAIPGWLRLTSDDDIHLLIQRDPSLVAQEDKVRAAVGVDNSA
                     QFFVVRGKTPETVLQRAEALGKKLDALNGTPNSIGSYQSVVQFVPSAKQQNEDRALLA
                     QHVFNDPAALRATLLQAGFKDEVADAWLAAYAKPQPLLTVDTWLAAPWSQPYRHLWLG
                     EADSATKAYAAVVIPQGVTPQNGPALIATAQAVPGVVFVDKAASVSKLFGAYRVDSGW
                     WLAGALALVLVLLTLRYARREVTVAGAPQDVSLAAARHDGPVRDARQDIFPGNTRQEV
                     SLWDRVHGGIAVTLPVLLAVGVTLAVFGYLHVPLNLFNWLALMLVLGVGANYAVFLRE
                     GCLRPDADLGAVWTGVLLSAATTLLSFGMLGMSAMPALKSFGATLALGIAVSVLLAPI
                     GMPTESRRAA"
     gene            complement(3316..4008)
                     /locus_tag="VHV99_20710"
     CDS             complement(3316..4008)
                     /locus_tag="VHV99_20710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HEX3637859.1"
                     /translation="MGTMNLRNVAAGALLALGTLTTLGVSGLAAWPTAASAAEPAQAA
                     GNPALVSQIASHLAQAGGVRAQFTQTQTLAAMKQPLVSTGSLLFFRDRGVIWQIDTPY
                     KATYVITDAGVAEVNANGQRVTTHSAQGARGVAQVSKMMRAMLGGDLSALYSQFDVEA
                     EGSAAQWRMQLTPNQPQIAQSIKGLQMNGGDYLQSLRITLANGDITKLDFANSKAVTE
                     PTPAERSLLGVQ"
     gene            complement(3996..4427)
                     /locus_tag="VHV99_20715"
     CDS             complement(3996..4427)
                     /locus_tag="VHV99_20715"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX3637860.1"
                     /translation="MTGSPKVLTASATVEVPFHDVDAMNVCWHGHYLKYFEIGRAALL
                     RAFDYDYREMQASGYLWPIVEAHLKYVRPAIYGQALEVRTELLEHENRLKIGYEIVDC
                     ASGTRLTKGYTIQVAVDAATQELQFVSPPVVFEKLERVWER"
     gene            complement(4424..6064)
                     /locus_tag="VHV99_20720"
     CDS             complement(4424..6064)
                     /locus_tag="VHV99_20720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HEX3637861.1"
                     /translation="MAEHDLIEAPNVSANAKSNATAATAATAATVVTIGGRRLTIEEV
                     VAIAHHRAPVALSADPAWRARIQRGADFLRRHLAAGATVYGVNTGYGDACVVDVPMEL
                     VEALPLQLTRYHGCGMGQYLDDGQTLAVIAARLNSLAYGFSGVRPVLLERLADLINHR
                     VLPRIPSEGSVGASGDLTPLSYVAAALAGERDVMFEGTLRNVRDVWTELGSTPLTLAP
                     KEGLALMNGTAVMTGLACLAFARADHLTRLTARLTALSTVALDGRAAHFDAMLFEVKP
                     HAGQAEAAAWIRDDLSGRDDTPGHRLQDRYSIRCAPHVIGVARDALSWVRRDVENELN
                     SANDNPLIDPDNERVLHGGNFYGGHIAFAMDSLKVAVANLADLMDRQLALLVDVNFNN
                     GLPRNLSGAAPARAAINHGFKAVQISSSAWTAEALKNTMPASVFSRSTEAHNQDKVSM
                     GTIAARDCLRVLELTEQVAAAHTLATVQAARLRLKIDSATPVPAPLRDFIDSVTAQSP
                     FVDEDRALESDLRALTARIAACELLTDYPSDYRGGSNA"
     gene            complement(6051..>7042)
                     /locus_tag="VHV99_20725"
     CDS             complement(6051..>7042)
                     /locus_tag="VHV99_20725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX3637862.1"
                     /translation="DDTDQPADTLADEPRPEAWWRIAERGSHLGMSLLALSCTLFGRR
                     FTALWLHPIVAYFLLTGRAARAASENYFTRLHAVAPSVDTPRPGWLSAYRHMLAFAQS
                     GFDKLAAWSGRVNNADVEFEDPSAFEALVASGKGALVIGAHLGNLEMTRALAAQGAYA
                     KVTAVVYTEHARRFNSVLASANSQFARHLLEVSDFGPETAMMMQERVDAGELLVIVGD
                     RVPAHEAGRTTEAQFLGSTASFAQGPYVLAHALGCPVYLFFCLKEHDGYRLYFEPFAE
                     RIELPRRDRAQHLAAWAQRYAARLEHYCRKAPYQWFNFFDFWASPKRGAHGRT"
BASE COUNT         1278 a         2337 c         2265 g         1162 t
ORIGIN      
        1 cccgcgagtc tcaccgccgc ttcttcgcgg ctcatcagga acacggcggc gccttcgccg
       61 acgttgatcc cgcaacgatt gcggctcatt ggattgctgc gcacgttgct ggtcgattcg
      121 agcgatgcga agccttgcac cgtcaactcg cacagcgaat cgacgccgcc caccaccacc
      181 gcgtcgcaca gccgcaattg caacaaccgg cgcgcggacg caaaggcctt cgcgctcgag
      241 gtacaggcag tcgagatcgt gaacgccgga ccgtgcacgc cgagcgcggc ggcggcgaac
      301 ggcgcggcgg tgccgatctc catctgccgg taactgaagt tggcgggcag cttgcccgct
      361 tgcgcgtgat agacgaacgc ggcttcggcc gcttcgatac ccgaggtgct ggtgccgagc
      421 accacaccga tccgatgcgc gccgtaacgt tcacgcgccg caatgatggc gggctcgatc
      481 tgcgtcagcg cggcgagcag caagcggtta ttgcggcagt cgtagcgtgc cagcccggcg
      541 cgcggcgcca gatcgagagg cgtgagcacg ctgcccacaa acgcctcgcc aatgcccgta
      601 tgtgcgttcg ccatgccggg tgcctgacct gcggcaagcg ccggcacgat cgtatcgagg
      661 tcgccgccga gcgcattgat catgcccagc gcgtgcaaat aaactgatgg cgccttcatg
      721 cggccctcct tgattccgtt ggcatgccga tcggtgcgag caacaccgat acggcaatgc
      781 cgagcgcgag cgtggcgccg aaactcttca gcgcgggcat cgcgctcatg ccgagcatgc
      841 cgaacgacaa cagcgtggtg gccgccgaga gcagcacgcc cgtccacacc gcgccgagat
      901 cggcgtccgg gcgcaaacag ccttcgcgca gaaacaccgc gtagtttgca ccgacgccga
      961 gcaccagcat cagcgcgagc cagttgaaaa gattgagcgg cacgtgcagg tagccgaaca
     1021 cggcgagcgt gacgccgacg gcgagcagca cgggtagcgt cacggcgatg ccgccgtgta
     1081 cgcggtccca aagggagact tcctgccggg tattacccgg gaagatgtcc tgccgcgcat
     1141 ccctcactgg gccatcgtgc cgcgcggccg ccaaggagac gtcctgcgga gcgcccgcga
     1201 cggtgacttc ccgccgagcg taccgcagcg tcagcagcac gagcacgagt gcgagcgcgc
     1261 ccgcgagcca ccagccgcta tccacgcgat atgcaccgaa cagcttcgac acgctcgccg
     1321 ccttgtcgac gaatacgacg ccgggcaccg cctgcgcggt ggcgatcagc gcggggccgt
     1381 tctgcggcgt cacgccttgt ggaatcacga ctgccgcata ggccttggtg gccgagtcgg
     1441 cttcacccag ccacaagtgc ctgtacggct gcgaccacgg cgcggcgagc cacgtgtcga
     1501 ccgtgagcag cggctgcggc ttcgcatatg cggcgagcca ggcgtcggcg acctcgtctt
     1561 tgaagcccgc ctgcagcagc gtggctcgca atgccgcggg atcgttgaat acgtgctgcg
     1621 cgagcaatgc acggtcttcg ttttgctgtt tcgcggacgg cacgaattgc accaccgact
     1681 gatagctgcc aatactgttc ggcgtgccgt tcaacgcgtc gagcttcttg cctaaagctt
     1741 ccgcgcgttg caacacggtc tccggcgtct tcccacgcac cacaaaaaac tgcgcgctgt
     1801 tatcgacgcc aaccgccgcg cgcaccttgt cttcctgcgc gaccagcgaa ggatcacgct
     1861 ggatcagcag atggatatcg tcgtcgctcg tcagacgcag ccagccggga atggccacga
     1921 tcagcaacag cgccgcgacg aaccatgcac gcttgccgcc gatcatgcga tgccataccg
     1981 tcagcactcg cgccgcgccg gcgaacacgc gctgtgggct gcgcttcggc gcgcgcgtaa
     2041 gcagcgcggg caacaatgac agcacggagg caaacgccgt cacaatgccc acgatcgcaa
     2101 agcaggcaat ctgcttgagc gcggggaacg gcacccacgt gaggatcgca tagccgagca
     2161 ggctggtggc aagcgcaacc gtcaaggccg gacgtaccgc acgcgcgccg cggtgtgaat
     2221 cccaatcacg ccccgcgccg agatagacca cgaaatactg gatcgaataa tcgaccgctt
     2281 cgccgatcag gctcgcgccg aacaccagcg tcaacagatg cagttgaccg aataccagca
     2341 tcgtcaccgc gagcgcgcag acaatgccga gcgccgtcga aacgaaaccg agcagcaaca
     2401 gacgcggcga gcggaacacc cacatcatca acagcgcgat cccgcacagc gacgcgacgc
     2461 caatcaggtg gacctcgtgt tccgaggcgc tgcgtgccga ttccgcgtag aacaccgcgc
     2521 cggttcgcgc caccgacacg tcggggaagg cctgcttcaa cgcgctttcg ccttgtgcga
     2581 gcgcggcgag cacggcgcgc tgcgttttcg attcgtaggc cgagcccggc agcgtggcga
     2641 tgatcagcac gctggtcgcg gtgccgcgat gcgacaccag catgttgtct tcgacttcga
     2701 gatccgacgt ggcgagcggc aaaccgccga gccagtgttc gagccagccg aacggatcgt
     2761 cggcgagcgg cgtggtcagg ccgccgcgca atgggctgta gatgcgctgc gcgagcgcgt
     2821 ccctcaatcc tccctgggga cttccttcgg gacgggcggc ttcctgcgac gcgccgtgca
     2881 acgttgggct tgacgcattg ctctgcgcaa gcgccgcgcg gtcagcgggc gccagcaaac
     2941 cgaaacggta cggcatgtac aacgcagcga tctgcgacag atcgaacggc ggcaattccg
     3001 ccgtcaccga accaaacgcc ccgctcttct gcaatgaagc gccgagttgt ttcgctgccg
     3061 ccttcgcgtg cgcgtcgtcg ttgctggtga cgagaaaaac cgtgcgatca cccagcgcgc
     3121 tcgccaacgt atcgaccgct ttttcggcga ccggatccgc ttcagtggca ggaagcaagg
     3181 ccagcagatt ggtctgcagc ggcgacggtc ccgcgaatcg ccaaccgcag tacagcgcgg
     3241 cggcgagcgc gagcagcagc cacgcggcgc gcacgcccca tgcctgtttc gcggaccgct
     3301 gttgcagcac ctgcattact gcactccgag caaactgcgc tcagccggcg tcggttcggt
     3361 gaccgccttg ctgttcgcga agtcgagctt cgtgatgtcg ccgttcgcga gcgtgatgcg
     3421 caagctctgc aagtagtcgc cgccattcat ctgcaaaccc ttgatcgact gcgcgatctg
     3481 cggctggttc ggcgtgagtt gcatgcgcca ctgcgcggcg ctgccttcgg cctctacgtc
     3541 gaattgcgag tagagcgccg acagatcgcc gccaagcatc gcgcgcatca tcttcgaaac
     3601 ttgcgcgacg ccgcgcgcgc cctgggcgct atgcgtcgtc acgcgctggc cgttggcatt
     3661 gacttcagcg acgcccgcat cggtgatgac gtaggtggct ttgtatggcg tgtcgatctg
     3721 ccagatcacg ccacgatcgc ggaagaacag cagcgagccc gtgctgacga gcggctgctt
     3781 catcgcggcg agcgtttgcg tttgtgtgaa ttgcgcgcgg acacccccgg cttgagcaag
     3841 atgcgaggcg atctgcgaga ccagcgccgg gttgccggcg gcttgcgcgg gttctgctgc
     3901 cgacgccgcc gtcggccacg ctgccaggcc tgagacaccc aaggtggtta gcgtgcctaa
     3961 tgcgagcaac gcgcccgccg ccacattgcg caggttcatc gttcccatac gcgctccagc
     4021 ttttcgaata cgaccggcgg cgacacgaac tgcaattcct gcgtcgcggc atcgaccgcg
     4081 acctggatcg tgtagccctt cgtcagccgc gtaccggacg cgcaatcgac gatttcatag
     4141 cctattttca ggcggttttc atgttcgagc agttccgtac gcacttcgag cgcctggccg
     4201 tagatggccg ggcgcacata cttcagatgg gcctcgacga tcggccacag gtaacccgag
     4261 gcctgcatct cgcggtagtc gtaatcgaag gcgcgtaaga gcgcggcgcg gccgatctcg
     4321 aagtacttca gataatggcc atgccagcac acgttcattg cgtcgacgtc gtgaaacggc
     4381 acttcgaccg tcgcgctggc tgtcagcacc ttaggggagc cggtcatgcg ttcgagcctc
     4441 cgcgatagtc gctcggatag tcggtcaaca actcacatgc ggcgatgcgc gcggtcagtg
     4501 cgcgcaggtc gctttccaaa gcgcggtctt cgtcgacgaa cggcgattgc gccgtcacgc
     4561 tgtcgatgaa gtctcgcagc ggcgcgggaa ccggcgtggc gctgtcgatc ttcaagcgca
     4621 gacgtgcggc ttgcaccgtc gccaacgtat gcgcggccgc gacctgctcg gtcaactcca
     4681 gcacacgcaa acagtcgcgc gcggcgatcg tgcccatgct gaccttgtcc tgattatgcg
     4741 cctcggtcga acgcgaaaag acgctcgcgg gcatcgtgtt cttcaacgct tcggccgtcc
     4801 atgcggacga tgaaatctgc acggccttga aaccatgatt gatcgcggca cgcgcgggcg
     4861 ccgcacccga cagattgcgc ggcaagccgt tgttgaaatt cacgtcgacc agcagcgcga
     4921 gctgccggtc catcagatcg gcgagattgg cgaccgcgac tttcagcgag tccatcgcga
     4981 aggcgatatg gccaccgtag aagttgccgc cgtgcagcac gcgctcgttg tccggatcga
     5041 tcaacgggtt gtcgttcgcg ctgttcaatt cgttctcgac gtcgcggcgc acccacgaca
     5101 acgcatcgcg cgccacgccg atcacatgcg gcgcacagcg aatcgaatag cggtcttgca
     5161 gacgatgccc gggcgtatcg tcgcgcccgc tcagatcgtc gcgaatccac gcggcggctt
     5221 cggcctggcc ggcgtgcggt ttcacttcga acagcatcgc gtcgaagtgc gcggcgcggc
     5281 cgtcgagtgc aacggtggag agcgccgtga gacgcgcggt gagacgcgtc agatgatcgg
     5341 cgcgtgcaaa cgcgagacag gcgaggcccg tcatcaccgc ggtgccgttc atcagcgcga
     5401 ggccttcttt cggcgcgagc gtcaacggcg tagacccaag ttcggtccac acgtcacgca
     5461 cgttgcgcag cgtgccttcg aacatcacgt cgcgttcgcc ggcaagcgcg gcggctacat
     5521 acgagagcgg cgtcagatcg ccgctcgcac cgaccgagcc ttccgacgga atgcgcggca
     5581 acacgcgatg attgatcaga tcggcgaggc gttcgagcaa cacggggcgc acgccggaaa
     5641 agccgtaggc cagcgagttg aggcgcgcgg cgatcaccgc gagcgtttgc ccgtcgtcga
     5701 gatactggcc catgccgcag ccgtggtagc gcgtcagttg cagcggcagg gcttcgacca
     5761 gttccatcgg cacgtcgacc acgcatgcat cgccatagcc ggtgttgacg ccgtacacgg
     5821 tggcgcccgc cgccagatgc cggcgcagga aatccgcgcc gcgctggatg cgcgcccgcc
     5881 atgccggatc ggcgctcaat gcgaccggcg cgcgatggtg cgcgatcgcg acgacctctt
     5941 cgatcgtcag cctgcggccg ccgatcgtca ccaccgttgc cgccgttgcc gccgttgccg
     6001 cagttgcgtt tgattttgca ttcgcgctga cgttcggcgc ctcgatcaga tcatgttcgg
     6061 ccatgtgcgc ctcgtttggg gctggcccag aaatcgaaga aattgaacca ttgataaggt
     6121 gccttgcgac aataatgttc gagccgcgcc gcgtaacgct gcgcccatgc ggcgagatgc
     6181 tgcgcgcggt cgcggcgcgg caactcgatg cgttcggcga acggctcgaa atacagacga
     6241 taaccgtcgt gctctttcag gcagaagaac agatagacgg ggcagcccaa cgcatgcgca
     6301 agcacgtagg ggccttgcgc gaacgatgcg gtcgagccga gaaattgcgc ttcggtcgtg
     6361 cggcctgctt cgtgtgccgg cacgcggtcg ccgacgatca ccagcagctc gcccgcatcg
     6421 acacgctcct gcatcatcat cgcggtctcg ggaccgaagt cgctgacttc gagcaggtgc
     6481 cgcgcgaact ggctattggc cgacgccagc acgctattga agcgccgcgc gtgttcggtg
     6541 tacaccacgg cggtgacctt ggcgtaggcg ccctgcgcgg cgagcgcgcg ggtcatctcc
     6601 aggttgccga gatgcgcgcc gatcacgagc gcgcctttac cgcttgccac caatgcttcg
     6661 aatgccgaag gatcttcgaa ttcgacgtcg gcattgttga cgcggccgga ccaggcggcg
     6721 agcttgtcga agcccgattg cgcgaacgcg agcatatggc ggtacgcgga cagccagccc
     6781 ggacgcggcg tatcgacgga tggtgcgacc gcgtgcagac gcgtgaagta gttctccgac
     6841 gcggcacgcg cggcgcggcc cgtcagcaga aaatacgcga cgatcggatg cagccacagc
     6901 gcggtgaagc gacggccgaa cagcgtgcag ctcaacgcga gcaggctcat gccgaggtgg
     6961 ctgccgcgtt cggcgatgcg ccaccaggct tcgggccggg gttcatcggc gagcgtgtcg
     7021 gcgggttgat ccgtgtcgtc cg
//
LOCUS       DATASH010000775        16097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_127995,
            whole genome shotgun sequence.
ACCESSION   DATASH010000775 DATASH010000000
VERSION     DATASH010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 16097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16097
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127995"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VHV99_20730"
     CDS             <1..247
                     /locus_tag="VHV99_20730"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX3637863.1"
                     /translation="TAVLNNLRLVLEDAGTDFSRLVRVQAYLSDMSDFQRFNAVYAEM
                     MGTAAPVRTTIGCALNGVLVEIDAVAEPGDSSVDPQK"
     gene            274..375
                     /locus_tag="VHV99_20735"
                     /pseudo
     CDS             274..375
                     /locus_tag="VHV99_20735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013700100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            564..2018
                     /locus_tag="VHV99_20740"
     CDS             564..2018
                     /locus_tag="VHV99_20740"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEX3637864.1"
                     /translation="MSDCYDVVIRNGIIVDGTGAARYDGDVGIKGDRIVCIGNLQGTR
                     ATTEIDASGLAVAPGFIDAHTHDDRLMLSGGDMAPKVSQGITTVVGGNCGVSLAPMPR
                     PIPDPVTPPLNLLDESGSWFRFASFAEYLDALRAQPAATNCAMLVGHSTLRVATMDDL
                     TVPANPDEIASMRALVDEAMRAGAIGVSTGLFYEPSIASPTEEVIEICQPLREHGGVY
                     CTHMRNEGDQVIESLLESFRVGREVGIPVVISHHKVVGVKNYGRSAETLKLIAENMEK
                     QEICLDCYPYPASSTILSVQRARNATRVTVTWSKGLPQYAGMDLTDIVAELGGTQEEV
                     IDRLLPAGAIYHIMDEQDVQRILAFDQTMIGSDGLPHDEAPHPRLWGSFPRVLGHYGR
                     SLGLFSLEIAIHKMTGLTARNFGLAGRGVVQEGAFADLTIFNPETVADAASFAKPIAP
                     AKGIHIVLVNGVVVWRDGGPSGARPGQVLRRERV"
     gene            complement(2041..2976)
                     /locus_tag="VHV99_20745"
     CDS             complement(2041..2976)
                     /locus_tag="VHV99_20745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3637865.1"
                     /translation="MATHFDLTDVQLIVNVGDSLSLTRAAEKTHLSVPAASMRIKNLE
                     DYFRTRLLYRTNQGVTLTRSGEELMRHARILMAQVEQLHGDMQEFARGVKGRLRVLGN
                     TTAMMEVMPNILSRFLASHPDVDLELRECGSQAIAKAVSEGSADVGIVAGCPPAENLH
                     YLPCRDDRLVLIVPSGHVLEGREAVSFVETLTFDYVGLSEWSAIHGFLLRAASAAGRQ
                     FRFRAEMGNFDAVCRMIEATVGIGVIPESVARRLVPTMRITAIALTDSWALRRLHVCV
                     RDLEALPFFARELVDMFAAEGRAGEAVEVESPAGV"
     gene            complement(3089..3898)
                     /locus_tag="VHV99_20750"
     CDS             complement(3089..3898)
                     /locus_tag="VHV99_20750"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEX3637866.1"
                     /translation="MKPKNMLMDTMKSGRTALGVWANDPETAELCAHLGFDWIMIDMM
                     FTGMDFHDVQNMIRTCEAAGITPVVRMQTNPWHGYDARIGVDLSRLQGLGAEYVLISH
                     SGLQEIDEALDIARDWHRRALWIHPFNSREWEQRTDEMAQATYIIPHVESQGGIDDFD
                     KVLDHPDLKMFFFAMTDLSKVLGGNAKKPDFNSPELWKLVDRGVKKAHERGIMVGANT
                     SYAYTLEEMRQRVKRLHDAGMNFIMIQGAQFLFQVAMMEFLPNVRKDLGLV"
     gene            complement(3958..5688)
                     /locus_tag="VHV99_20755"
     CDS             complement(3958..5688)
                     /locus_tag="VHV99_20755"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX3637867.1"
                     /translation="MSDLPFAERHAMQRHRLMQRLPAALAVVLICSAVVILVIAPLFA
                     VLYRAVFDADTPGASLTSRYFAEVLSKRIYWDALVNTLAVSGGAALLATLLGVLFAWI
                     FERTDTWMRGVLQWISQLPIFIPPFVGAVAWALLAAPRSGIMNRMLAAVGIPGVLDVY
                     TRWGMLIVIGLYLAPYVMMIVAAALRSMDPGLEEAAQVCGLTPLQTMRRITVPLLAPA
                     ILSGAVLSFTIAIGLFGTPVVLGWSRQILMLTSRIWIGSQEVPPNYGVMAVLSIYLLG
                     LSTLTIWLQRVLIGKRVYTTISGKGFRPRLVELGGWRWATVILATLYVVSTIIAPIAI
                     LVAAALSRYTWSGSYSLANAIGYLQSDDVWFTLQNSVVISVISASVATLAGIVIAWIV
                     VRTRLPGKGVLEYVILLPISMPGIAFGVGMLLFWINAPIPVYGTTLIIVFAFVGRFTA
                     YAVRSVSASLIQVHPELEESARVCGYGPLRTFTHITLPLIRASIVAAWLLLFSFFMTE
                     LSMVVILYTAADRTFSVLAFEAWNVGDFSRLASYSLLQMAVGIVFMAILKTCFRSWNS
                     PRSMQPVTPN"
     gene            complement(5691..6761)
                     /locus_tag="VHV99_20760"
     CDS             complement(5691..6761)
                     /locus_tag="VHV99_20760"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3637868.1"
                     /translation="MSIVIRRLQKSFGVQPVLKSVSETFADAQISVLLGASGCGKTTT
                     LRCIAGLERPSGGEIVVAGRTVYQDSPALWIAPERRHMAMVFQSYAIWPHMSVFENVR
                     LPLRAAGIGGAKARDAVMATLQLVGLDAFASRSATQLSGGQQQRVALARAIVSEAPVI
                     LMDEPLSNLDAKLRVEMRIEIRALQRRLGRTILFVTHDQEEAMSIADTVYLYHNGTVI
                     QKGAPKDLYDHPASRHAAEFLGRANIIANYEWQMAANGPVFAMPGGLALPAAATQTQA
                     PQPGELLCIRPEKWRVRQAGTPGAIAGRVVEASFVGNRTEYRIDTAFGQMSVVQLDAG
                     PRLQGDAVGLEVSPADIRVIGA"
     gene            complement(6770..7819)
                     /locus_tag="VHV99_20765"
     CDS             complement(6770..7819)
                     /locus_tag="VHV99_20765"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX3637869.1"
                     /translation="MLLVRMRTWLSVLAALILMLRLVSADAFASDPALIAAAKKEGSL
                     VVYGCDPPQTPVYLDAFKKLYPEIKVSSYVAGCWQIYNRHITERNAGKQAADAFFSLE
                     DVFTRMQNEKLLDPYHSPELKNFPADAAPAGKDYQRVKTLILGVGANKAYLNGMQPPT
                     DWMDFANPKKEWQNQITFYDPRTSSAAFSLLATLYQNFGEQQTVAIYQGLIRAGAELT
                     PTTPAGLAKMLSGEKPLMFYVVNNHYSGAVAKGAPLEFIVPKSGTVSTPFSVAVLAGA
                     PHPAAAKLFVDFMMSDAQAIIVKANEYALRNGSTPPQGMPKLSAVKVLPLDIDAALRQ
                     QTQLIEIWQHATGIR"
     gene            8089..9489
                     /locus_tag="VHV99_20770"
     CDS             8089..9489
                     /locus_tag="VHV99_20770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX3637870.1"
                     /translation="MAHASAGRPTLSQTFAAFAHTLDPATVPHHVRTRAKHLILDAVG
                     IAFASHRNEFADVMLRGVKAFREHGAAPVIGCSDTQTVRNAVLLNAALMHGLDYDDTH
                     MKGIVHATAFCAPAALCVANEVGASGRAALTAYLVGMEVATRLGAAAKGGFHHAGYHP
                     TGVLSHFSAALIAGRLYRLDVDQLAAAQGIAASTASGVQVFLEEGTWSKRLHPGWGGV
                     GGITAARLAQAGFVAPSRPYEGRYGLFDSHLQQHAGEVDYMVASAGLGEVWETEQMAI
                     KPYPVCHFLHSIAEAGADLAREAQIDLGRISRIRALLPEPVMPIVSEPLAAKRRPTTN
                     YEAKFSAQYVAAASLVRGRFGLAELEPDALADERILSLSARVECVADPDSAFPAYYSG
                     GLIVDTDDGRQHQRYYRINKGAGERALSNEAIVAKYLENACWSISHARAEQVREAVLA
                     LDELQTSELWAVLRTR"
     gene            complement(9541..10293)
                     /locus_tag="VHV99_20775"
     CDS             complement(9541..10293)
                     /locus_tag="VHV99_20775"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX3637871.1"
                     /translation="MTVANETDVKSARRVVEIFQLFAELRQAVPLALISKRLALPKSS
                     CLALLKTLESNGYLYRVHESADYYPTRRIFDEARIIVDNDPLLSHVRPVLQELCNETG
                     ETVFLATRTGHQAHYLEVVQSGQTLRYAATVGEKRPLHIGAAGQALLGAMDETARDAL
                     IDKLEFEQYAPNTVTSKPRYKRLIEQGKERGWFVSIGGYQSEIASIGHYVWINGEAYA
                     IVVGAPTQRVKGNETSIGRAVARYCKKISNRA"
     gene            10531..11370
                     /locus_tag="VHV99_20780"
     CDS             10531..11370
                     /locus_tag="VHV99_20780"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX3637872.1"
                     /translation="MTSSIVVKPLAGSLGAEIFGVNLSTELDPATVAQIRRAYHEHLV
                     IFFRDQDASDHDLARFARYFGELAVLPPHRQHPGSLPELLIIDKKPEDSLVFGWEWHS
                     DTTHLEIPPLGSVLAARLLPTVGGDTLFSNQYMAYDTLSDGMKAMLKDVRAVHSNGHI
                     LASLEADDVLAPGQGYNSHTTLSAVHPVIRTHPDTGKKALFVNSLHTERFDNMTVAES
                     TPLLRFLFEHATRPEFTCRFRWYPGSIAFWDNRCCQHLALDDYPGQRRLMHRVQIRGT
                     RPE"
     gene            11502..12815
                     /locus_tag="VHV99_20785"
                     /pseudo
     CDS             11502..12815
                     /locus_tag="VHV99_20785"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            13045..14031
                     /locus_tag="VHV99_20790"
     CDS             13045..14031
                     /locus_tag="VHV99_20790"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HEX3637873.1"
                     /translation="MKRTARLRALINAPQILALHGVYDGFSARLVEHCGFTSAFITGS
                     GISESRLGQPDVGLMGLEENVAAARSLSACTNLALLADGDTGYGNAVNVYHTVKAFEQ
                     AGVAGLMLEDQVWPKRCGHMKGKEVIPREEMVEKIHAAVAARVDPDFIIKSRTDALAS
                     DGMDEVIRRLNAYAEAGADLLFCDALVTEEQISTVARNVSKPLCVNMGFGIRRRATTP
                     LLSAAHLQDLGVAVVIFPRMLTACALMGMKQGIQLLQQSFTSGNVIDRPDVLVSFEEL
                     NEIMGIEQVQELEARFLTPAQKAAKYKRDGAQNGTVDGLSDDRSARNGVSAA"
     gene            complement(14108..15016)
                     /locus_tag="VHV99_20795"
     CDS             complement(14108..15016)
                     /locus_tag="VHV99_20795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX3637874.1"
                     /translation="MDVRQLRYFATVVDTGSLSKAAVRLSISQPSLSQQIVGMEDDLG
                     VPLLLRSPSGVRPTEAGMALYRHARTILKQFEQMRDDVRRGEHSESGQVAVGLPTSIA
                     AVIAVPLFNRLQSTYPNIQLQLFESMSGYLIELLANGRLDMAVLFRETETRGLTVSPL
                     FNEHLSLCGVPWVGDANGSSVPLSLVSGVPLVLPGKANGLRLLIERTFAREGLVLNVV
                     ADIDSLPTMLTIASEGEVGSILSGALARQPGAALLGRRLVEPSLERMVSLCVPNAVPQ
                     NAASRAVQNTIEQIVREEEVKWDTVA"
     gene            complement(15211..>16097)
                     /locus_tag="VHV99_20800"
     CDS             complement(15211..>16097)
                     /locus_tag="VHV99_20800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020657044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEX3637875.1"
                     /translation="RWRMLESAAASAADAVFIDLEDAVPPSEKSVALESAIRALGQLD
                     WGAKHVAVRVNVTQAGVLESELSALAGEAARLDAIIVPKVESTRVLQQVETLLDHHRG
                     VHPQLAIEALIETARGMVNVDAIAAAGGRLAALHFGVGDFSASIGARNVEVGGTHPGY
                     AVTRRDEIGNYVTAPLDLWSYPMMRILVAARAFNRRAIDGPCGAFRDPVLTRSFALKA
                     AAMGFDGKQVIHPEQIAATQAAFTPTDAEVRAAEATIDAMAQAEAVGLAAVSVDGKMV
                     DYANLRMAQRLLAMAARP"
BASE COUNT         3098 a         5000 c         4876 g         3123 t
ORIGIN      
        1 caccgccgtg ctcaataacc tgcgcctggt gctggaagac gccggtaccg atttcagtcg
       61 actagttcgc gtccaggcct atctgtcgga tatgagcgat ttccagcggt tcaacgctgt
      121 ctatgccgaa atgatgggca ccgccgctcc tgtacgaacc accatcggat gcgcgctcaa
      181 cggcgtactt gtggaaatcg atgccgttgc ggagcctggg gacagttctg ttgatccgca
      241 aaagtaacga agtcatccat tcttcgaggg ggaagcatca agtccagaac gaatgtgatg
      301 ctcggcttca aacggttcag gaatgccgcg gccacgattt caggcatcga attgatgcat
      361 cgcattcgca agggtctgag cgatccgcaa catgaatata ctcgcgcgct tatcgacgtc
      421 gcgccgggac gcgagtggga tttccggaac ttctgccctg cggcggcggc ttgaccgatc
      481 gtcgtcacgc gctagtgacg acgggcatcg cggcgcgccc cgctgtatag accgactgat
      541 aaattcaata cataggaatc cagatgagtg attgttacga tgtagtgatc cgcaatggaa
      601 tcattgtcga cggaacgggc gccgcccgct atgacggtga tgtgggtatc aaaggcgatc
      661 gtattgtgtg catcggcaat ttgcaaggga cccgcgcaac aacggagatc gacgcatcgg
      721 gccttgcggt tgcacccggc tttatcgatg cgcatacgca cgatgaccgc ctgatgctat
      781 ccgggggcga catggcgcca aaagtcagcc agggcatcac taccgtggtg ggcggcaatt
      841 gcggcgtttc gctggcaccg atgccgcggc cgattcccga tccggtcacg ccgccgctta
      901 acctgcttga tgaaagcgga tcgtggttcc ggttcgccag ctttgctgaa tatctggatg
      961 cgttgcgtgc tcagcccgct gcgacgaatt gcgccatgct ggtcggtcat tcgaccttgc
     1021 gtgtcgctac catggatgat ctcaccgtac cggcaaatcc agatgaaatt gcttccatgc
     1081 gcgcgctggt cgatgaagcc atgcgcgccg gcgcgattgg cgtgtcaaca ggattgttct
     1141 acgagccttc gatagcatca cccacggaag aggtgattga aatatgccag ccgctgcgcg
     1201 agcacggcgg ggtgtattgc acgcacatgc ggaacgaagg ggatcaggtc attgagtcac
     1261 tgcttgaaag tttccgcgtc ggccgcgaag ttgggatacc agtcgttatc tcgcatcata
     1321 aggtggtcgg tgtcaaaaat tatggccgct ccgccgaaac actgaagcta atcgccgaga
     1381 acatggaaaa gcaggaaatt tgtctggatt gctatcctta tccggcgtct tcgaccattt
     1441 tgtctgtaca gcgcgcacgc aacgcaaccc gagtgaccgt cacatggtca aaaggtctac
     1501 cccaatacgc cggcatggat ctgacggaca tcgttgcgga actcggtggt acgcaggagg
     1561 aagtgatcga ccgtctgctg ccggccggcg cgatttacca cataatggac gagcaggatg
     1621 tgcagcgcat tttggcgttc gaccagacca tgatcggctc cgatggattg ccgcacgacg
     1681 aggctccaca cccgcgtctg tggggaagtt tcccgcgtgt tctcggacac tacggacggt
     1741 cattgggcct gttttccctt gagatcgcca tccataaaat gaccggactg acggcaagga
     1801 attttgggct cgcaggtcgt ggtgtggtgc aggagggcgc cttcgctgac ctgactattt
     1861 ttaatcctga aacggttgcc gacgcagcgt catttgccaa accgattgcg ccggccaaag
     1921 gcattcatat cgtgcttgtc aacggcgtcg ttgtgtggcg tgacggcggg cccagcggag
     1981 cacggccggg ccaggtgcta cggcgtgagc gcgtgtagaa atgtcgcatt ccaacacgac
     2041 tcaaaccccc gctggcgatt ccacctcaac agcctcccct gcgcgcccct cagccgcgaa
     2101 catatcgacc agttcacgcg cgaagaaagg cagcgcctcg agatcgcgta cgcagacgtg
     2161 cagccggcgc aacgcccagc tatcggtcaa tgcaatcgcc gtgatccgca ttgtcggcac
     2221 gagcctgcgc gcgaccgatt cgggaatgac gccgatgccg actgtcgcct cgatcatccg
     2281 gcacacagca tcgaaattgc ccatctcggc gcgaaagcga aactggcggc cggcggcgct
     2341 cgccgctcgc aacagaaacc cgtgaatcgc gctccattcc gacaggccaa cgtaatcaaa
     2401 agtgagcgtt tcgacgaatg agactgcctc gcgtccttcc agcacatggc cgctgggcac
     2461 gatcagcacg agacggtcgt cacggcatgg cagatagtgc agattttccg cgggcggaca
     2521 acccgcgacg atgccgacgt cggccgagcc ttcggacacc gcctttgcga ttgcctgact
     2581 gccgcattcg cgcagttcga gatcgacatc gggatgagag gcgagaaacc ggctgaggat
     2641 gttcggcatc acctccatca tggccgtggt attgccaagc acgcgcagcc gccctttgac
     2701 gccgcgcgca aattcctgca tgtcgccatg caactgttca acctgcgcca tcaggatgcg
     2761 cgcgtgtcgc atcaactctt cgccggatcg cgtgagcgtc acgccctgat tggtgcggta
     2821 cagcaatcgg gtacggaaat agtcttcgag gttcttgatc cgcatgctgg ctgcgggcac
     2881 cgagagatgc gttttctcgg ccgcccgcgt caagctcaac gaatcgccca cgttcactat
     2941 caactgcaca tcggtcagat caaagtgcgt cgccatagcg ttgattgtct cctcagcggc
     3001 aaaccggggc acaggtatca cgattttcgc gtgacctata ttaccccgtc ctgcgagccg
     3061 cttcatggag acccccgccg tcatgcattc aaactagtcc gaggtccttg cgcacgttcg
     3121 gcaggaattc catcatcgcg acctgaaaca ggaactgggc gccctggatc atgatgaagt
     3181 tcattccggc atcatgcagc cgtttgacgc gctggcgcat ctcttccaat gtgtacgcat
     3241 aactggtatt tgcaccgacc atgatgccgc gctcgtgtgc tttcttcacg ccacgatcca
     3301 ccagtttcca cagttcgggg ctattgaaat ccggcttctt tgcattgccg ccgagtactt
     3361 tcgaaagatc ggtcatggca aagaagaaca tcttcagatc cgggtgatcg agtaccttgt
     3421 cgaaatcgtc gataccgccc tgactctcca cgtgtggaat gatataggtg gcttgcgcca
     3481 tctcatcggt gcgctgctcc cattcccggc tgttgaacgg atgaatccat agcgcgcggc
     3541 ggtgccagtc gcgcgcaatg tccagcgcct cgtcgatttc ctgcaggccc gagtgcgaga
     3601 tcagcacgta ttccgcaccc aggccctgca gacgcgacag atcaacgcca atacgcgcat
     3661 cgtagccatg ccacggattg gtctgcatgc gcacgactgg cgtgatgccg gctgcctcgc
     3721 acgtacggat catgttctgc acgtcatgga aatccatgcc ggtaaacatc atgtcgatca
     3781 tgatccagtc gaagccgaga tgcgcgcaca gttccgccgt ttcggggtcg tttgcccaca
     3841 cgccgagcgc cgtgcggccc gacttcatcg tgtccatcag catgttcttc ggtttcatta
     3901 ccctgctcct ccagacggcg cccagcaacg ggcagattcc gcagacggct gtctgcttca
     3961 attcggcgta accggttgca tcgagcgcgg cgaattccag ctgcgaaaac aggtcttgag
     4021 aatcgccata aagacgatgc caaccgccat ttgcaacagc gaatagctcg cgagccgcga
     4081 gaaatcgccg acattccacg cttcgaaggc gagtacggaa aatgtgcgat cagccgccgt
     4141 gtaaagaatc acgaccatcg acaattcggt catgaagaag ctgaagagca gcagccaggc
     4201 ggccacgatg ctcgctcgaa tcagcggcaa cgtaatgtgc gtgaacgtgc gcaagggtcc
     4261 atagccacac acgcgcgcgc tttcctcgag ttcgggatgc acctggatca ggctcgccga
     4321 cacggagcgc actgcatagg ccgtaaagcg gccgacgaac gcaaagacga tgatcagtgt
     4381 cgtgccgtac acgggaatcg gcgcgttgat ccagaacagc agcatgccca cgccgaatgc
     4441 gatgcctggc atcgaaatag gcaacaggat cacgtattcg agtacgccct tgcccggcag
     4501 ccgggttcgt acgacgatcc acgcgatcac aatgcccgcg agtgtcgcca cgctcgccga
     4561 gatcacggag atcacaacgc tgttttgcag ggtgaaccac acgtcgtccg attgcagata
     4621 gccgatggcg ttggcgagcg aatatgaacc ggaccatgtg tagcgagaca atgccgcggc
     4681 aacgaggatc gcgatcggcg cgatgatcgt cgacacgaca taaagcgtgg cgagtatgac
     4741 ggtcgcccag cgccagccgc cgagttcgac gagccgtggc cggaagccct tgccgctgat
     4801 ggtcgtgtag acgcgcttgc cgatcagcac gcgttgcaac cagatggtca gggtcgagag
     4861 tccgagcaga tagatcgaca ggacggccat tacgccatag ttcggcggca cttcctgcga
     4921 gccgatccag atgcgcgaag tgagcatgag aatctgccgc gaccagccga gcacgacggg
     4981 tgtgccgaac aggccgatcg cgatcgtaaa cgacagcacc gcgccggaga gaatcgcggg
     5041 cgcgagcagc ggcaccgtga tgcgccgcat ggtttgcaac ggcgtgaggc cgcacacctg
     5101 ggccgcctct tcgaggcccg gatccatgct gcgcaacgcg gccgcgacga tcatcatcac
     5161 gtacggcgcc agatagagac cgatgacgat cagcatcccc catcgggtat agacgtccag
     5221 cacgcccggt atgccgacgg ccgcaagcat gcggttcatg atgccgctcc tcggcgccgc
     5281 gaggagcgcc caggcgacgg cgccgacaaa cggcggaatg aagatcggca actgactgat
     5341 ccattgcagc acgccgcgca tccaggtatc ggtgcgctcg aatatccacg cgaacaacac
     5401 cccgagcagc gtggctagca gcgcagcgcc acccgacacg gccagcgtgt tgactaaggc
     5461 atcccagtaa atccgcttgc tcagaacttc ggcgaagtag cggctcgtga gcgacgcgcc
     5521 cggcgtgtcc gcgtcgaaca ccgcgcgata aagcaccgcg aaaagcggcg cgatcacgag
     5581 aatgacaacc gcgctgcaga tcagcacgac cgccagcgcc gccggtaacc gctgcatcag
     5641 ccgatggcgc tgcatcgcgt gacgctccgc aaaaggcaga tcggacaatc tcacgctcct
     5701 atcacgcgta tgtcggcggg gctcacttcg agaccgactg cgtcgccttg caggcgtggc
     5761 cccgcatcga gttgcacgac gctcatctgg ccgaacgcgg tgtcgatccg atactcggtg
     5821 cgattgccga cgaagcttgc ctcgacaacg cgtcccgcga ttgcaccggg cgtgccggcc
     5881 tggcgcacgc gccacttttc agggcgaatg cataacagtt cgcccggctg gggcgcttga
     5941 gtctgagttg ccgcggcggg caacgccagt ccgcctggca tcgcgaatac cgggccgttt
     6001 gccgccattt gccattcgta gttcgcgatg atgttggccc ggccgagaaa ctcggcggcg
     6061 tgacggctcg cgggatgatc gtagagatcc ttcggcgcgc ccttctggat taccgtcccg
     6121 ttgtgataca ggtagacggt gtccgcaatc gacatggcct cctcctggtc gtgcgtcacg
     6181 aacaggatgg tgcggccgag gcgccgctgc aacgcgcgga tttcgatgcg catctcgact
     6241 cgcagtttgg cgtcgagatt gctgagtggt tcgtccatca gaatcacggg cgcttcggac
     6301 acgatcgcgc gagcgagcgc gacgcgttgc tgctgaccgc ccgacaattg cgtcgcgctg
     6361 cgcgaggcga acgcatcgag gccgaccagt tgcaacgtcg ccatcacggc atcgcgcgct
     6421 ttcgcaccgc ctattccggc cgcgcgcaac ggcagccgga cgttttcgaa cacactcata
     6481 tgcggccaga tcgcgtacga ctgaaacacc atcgccatat ggcggcgttc gggcgcgatc
     6541 cataacgcgg gcgaatcctg gtacacggtc ctgcccgcaa ccacgatttc gccgcccgac
     6601 ggccgttcga gtccggcgat gcagcgcagg gtcgtcgtct tgccgcagcc gcttgcgcca
     6661 agcagcaccg atatctgcgc atcggcgaaa gtttcgctga ccgacttgag tactggctgt
     6721 acaccaaatg acttctgcaa gcggcgtatc acgatcgaca tggtgggcct caacggattc
     6781 ctgtggcgtg ctgccatatt tcgatcagtt gggtctgctg cctgagcgcc gcgtcgatgt
     6841 cgagcggcaa caccttgacc gccgagagct taggcattcc ttgtggcggc gtagagccat
     6901 tacgcaatgc gtattcgttc gctttcacga tgatcgcctg cgcgtcgctc atcatgaaat
     6961 cgacgaacag tttcgcggca gccggatgag gcgcgcccgc gagcaccgcg accgaaaacg
     7021 gcgtcgatac agtgccggac ttcggcacga tgaattcgag cggtgcgccc ttcgccaccg
     7081 cgccgctgta gtggttgttg acgacgtaga acatcagcgg cttttcaccg gacagcattt
     7141 tcgccagtcc cgccggcgtg gtgggcgtca gttccgcgcc cgccctgatg agcccttgat
     7201 agatcgcgac cgtttgctgt tcgccgaagt tctggtacag cgtagcgagc agcgagaacg
     7261 ccgccgatga cgtgcgcggg tcgtagaacg taatctggtt ttgccattcc ttctttgggt
     7321 tcgcaaagtc catccagtcg gtcggcggtt gcatgccgtt caaataggct ttgtttgcgc
     7381 caacgccgag aatcagcgtt ttcacgcgct ggtagtcctt gccggccggc gcggcatcgg
     7441 cgggaaaatt cttcagttcc ggcgaatggt agggatcgag cagtttctcg ttctgcattc
     7501 gcgtgaagac gtcctccagc gaaaagaacg catcggccgc ctgtttgccc gcgttgcgct
     7561 cggtgatatg gcggttgtag atctgccagc agccggccac ataggagctg accttgatct
     7621 ccggatacag cttcttgaag gcgtcgagat agaccggggt ttgcggcgga tcgcagccat
     7681 agacaacgag gctcccctct ttcttcgctg ccgcgatcag cgccggatcg cttgcaaacg
     7741 catcggccga caccaaacgc agcataagga tcaacgccgc gagcacgctt aaccacgttc
     7801 gcatccgcac tagaagcatg gttgtctccg aatcgtctta tgtcatccgc tgaggcgcgc
     7861 cggaatccgt tgcgcccgca tgtttgcgtc agcatggccg ataacggcgg catccggaat
     7921 ttcgatctgc tgaacgtcga tttaacgcgg gacaaagatt cgggttagtg cgtagatgtg
     7981 cgatgttccg cacaaaacat cttgcttgtg acaatggccg gtctataacg tgaatccagt
     8041 cggccctcga attcacagac tgctttcgac acacgatgga gctcaaagat ggcacatgca
     8101 tccgccggtc gccctactct ctcgcagacg ttcgctgctt tcgcgcatac gctcgatccg
     8161 gcgacggtgc cccaccacgt gcgcacgcgc gccaaacatc tgatcctcga cgcggtgggc
     8221 attgcgttcg cgtcgcaccg taatgaattc gccgacgtga tgctgcgcgg cgtgaaggcg
     8281 tttcgtgaac acggtgcggc gccggtcatc ggttgcagcg atacgcaaac ggtgcgcaat
     8341 gccgtgctgc tcaacgccgc cctgatgcac ggactcgatt acgacgacac ccatatgaaa
     8401 ggcatcgtgc acgccacggc gttctgcgcg ccggcggcgc tgtgcgtcgc gaatgaagtg
     8461 ggcgcgagcg ggcgcgctgc gctcaccgcg tatctggtcg gcatggaggt cgccacacgc
     8521 cttggcgcgg ccgccaaagg cggttttcat cacgccggct atcatccgac gggcgtgctc
     8581 tcgcacttct cggcggcgtt gatcgcgggc cggctatata ggctcgacgt cgaccaactg
     8641 gccgcggcac aaggcattgc cgcgagcacg gcctccggtg tgcaggtgtt tctcgaagaa
     8701 ggcacatggt ccaagcgctt gcatccgggc tggggcggtg tgggcggcat caccgcggcg
     8761 cggctcgcac aggcgggttt tgttgcgccg tcgcgcccgt acgaagggcg ttatggcctg
     8821 ttcgactcgc acttgcagca acatgcgggt gaagtggatt acatggtggc gagcgcgggg
     8881 ctcggcgaag tctgggaaac ggaacagatg gcgatcaagc cgtatccggt ttgccacttt
     8941 ctccattcaa ttgccgaagc cggggccgac ctcgcgcgcg aagcgcaaat cgatttgggc
     9001 cggatcagcc gcattcgcgc attgcttccc gagccggtga tgccgatcgt gagcgagccg
     9061 ctcgccgcaa aacggcggcc caccaccaat tacgaagcga agttcagcgc gcaatacgtg
     9121 gcggcggcct cgcttgtgcg cgggcgtttc ggcctcgccg agctggaacc cgacgcgctc
     9181 gccgacgaac gcattctgtc gctctcggcg cgagtcgaat gtgttgccga cccggatagc
     9241 gcatttccgg cctactactc gggcggtctg attgtcgata ccgacgatgg acgccagcat
     9301 cagcgctatt accgtatcaa caaaggcgcc ggagagcgcg cgctgtccaa cgaagcgatt
     9361 gtcgcgaagt acctggagaa cgcgtgctgg agcatcagcc acgcgcgggc cgaacaggtt
     9421 cgcgaagccg tgctcgcgct cgatgagttg caaaccagcg aactgtgggc cgtacttcgc
     9481 acacgctgag ccgcgccaaa cggaaacgac ggccggccct cggcgtggct tgccgcgttg
     9541 ctaggcccgg ttggaaatct tcttgcaata cctggctacc gctcggccaa tcgacgtctc
     9601 gttgcccttc acccgctggg tcggcgcacc gacaacgatt gcataggcct cgccattgat
     9661 ccacacgtag tggccgatcg aggcgatttc cgactggtat ccacctatcg acacgaacca
     9721 gccgcgctcc ttgccctgtt cgatcaggcg cttgtagcgc ggcttgctgg tcacggtgtt
     9781 cggcgcgtat tgctcgaatt cgagtttgtc gatcaacgcg tcgcgcgcgg tctcgtccat
     9841 tgctccgagc aacgcttgcc ctgcggcacc gatatgcagg ggccgctttt cgccgacggt
     9901 ggccgcatag cgcagcgtct gccctgattg cacgacttcg aggtaatgag cctgatggcc
     9961 cgtgcgcgtg gccagaaaca cggtctcccc tgtttcgttg catagctcct gaagcacggg
    10021 ccgcacatgc gaaagcagcg gatcgttgtc gacgatgatg cgtgcctcgt caaaaatccg
    10081 gcgcgtgggg taatagtccg cggattcgtg cacgcgatac agatagccgt tcgattcgag
    10141 cgtcttcagc agcgcgaggc agctcgattt cggcagcgcc agccgcttgc tgatgagcgc
    10201 aagcgggacc gcctgcctga gctccgcgaa caactggaaa atttcgacta cgcggcgtgc
    10261 gcttttgaca tccgtctcgt tggcgaccgt catggcgacc ccgaaaaaaa cgtaggagaa
    10321 tttaaggaaa acccgaattc gagccgatcc acgccgaaca ctcgcttgca cagtgccgga
    10381 ccttcaacta ttcttggttc aaggataaga atattcgttc acatatgtga acgcaagcaa
    10441 tttctcggaa cacatgatcg tcgtgattaa acgggacgcc gcacaggtcc gctgaaaacg
    10501 ctcgccacgt tttgcgagca ggagacacgc atgacatcga gcatcgtcgt caagccgctc
    10561 gccggttcgc tgggggccga aatattcggc gttaatctgt ccaccgaact ggatcccgca
    10621 acggttgcac agatcagacg cgcctatcat gagcacctcg tgatcttctt ccgtgatcag
    10681 gatgcatccg atcacgacct tgcccgtttc gcgcgctatt tcggcgaact cgcggtactg
    10741 ccgccgcatc gccagcatcc gggatcgctg cccgagttgc tcatcatcga caagaagccg
    10801 gaagactcgc tcgtcttcgg ctgggaatgg cattcggata ccacgcatct ggagattccc
    10861 ccgctcggct cggtactcgc cgcgcgcctg ttgcccaccg tgggcggcga cacactgttc
    10921 tccaatcagt acatggccta cgacacattg tccgacggca tgaaagcgat gctgaaggat
    10981 gtgcgcgccg ttcattcgaa cggccacatc ctcgcctccc tcgaagccga cgacgtgctt
    11041 gcaccgggcc agggctacaa cagtcatacg acgctatcgg cggtgcaccc tgtcatcaga
    11101 acgcatcccg acaccggtaa aaaagcgctg ttcgtcaact cgcttcatac cgagcgcttc
    11161 gacaacatga ccgttgccga aagcacgccg ttgctgcggt ttctgttcga acacgccacg
    11221 cgacccgaat tcacctgccg gttccgctgg tatccgggtt cgatagcgtt ctgggacaac
    11281 cgctgctgtc agcatctcgc gctcgacgac tatccgggcc agcggcgcct gatgcatcgc
    11341 gtgcaaatac gaggcacgcg gcccgaataa attgaaacgc cacccacgag aacgcgacgg
    11401 gcaacactgg aggagacatc atgtcgatcg cagaggccac catcgaaagg atgcccgtgg
    11461 gcgcggcgca ccgccggatg ttctggctgc taggcgcggg catgctgatc gatgcgttcg
    11521 aactgttcat gcagtcgggc gtggtcgcct cgatggtgca cacgcatttc accgacacgg
    11581 gcggtgtcgc acagtttatt tttgccacgt ttctcggcct tgcgatcggc tcggtcgtca
    11641 gcgggacgct tgccgaccgc tacgggcgca aatcgctcta tcagttcaac ctgctgctct
    11701 tcagcttcgc gacgctggcg acggcggccg tcccatcgtt tccgctcgtc gttgttttac
    11761 gcttcatcgc cggcatcgga ctcggcggcg aactggtggt cggctacgcg atcatggcgg
    11821 aaatgacgcc tgcgtattgc cgcggacgct ggggtgtcac gctcggactg ctcgtcaatt
    11881 gcgcgcagcc gctgtcggca ttcgttggcg cgtggtttct gcccatggtc ggttggcgtg
    11941 cgatgttcat cgtcggcgct gtgccggcgt tgatcgtctg gtatctgcgg cgcgcattgc
    12001 ccgagtagcc gcgctggctt gagcgccagg gccgcaccac cgaaatgcat gccgtgctcg
    12061 acgcgctatg gctcgagaat accggcgagc cgcaagcgcc tcgcgtgacg cagaccacct
    12121 tcgcacccga gcgcaaggaa ctgccgtgga cggctttgtt cagccgccac cgacttggcc
    12181 gcacgctttt ctgtttcgcc atcgtgtcgc tgattcttgc gagccagtat ggcttcattg
    12241 gttgggtgcc gacactgctg ttgcatcgcg ggcagtcaat cgtgcactcg ctctcctact
    12301 ccgcgatcat ggcgatgggc gcgccgatcg gcatgctgat tccaattttt ctggtcgacc
    12361 ggctcgggcg ccgtaccgtg gtggcgagcg cgtcgtttct cgccgcggcg gtcggcatcg
    12421 gctacgcgtt cgcgctcgat tcgcctgcat ggatactcgt cacaaccggt tttctcgaag
    12481 tgctcttcgt gcagatcacc gcgaatacgg tactcgccgt ctatctgccg gaactctttc
    12541 caaccgatgt gcgcggcacg ggtttcgggg cgtcgacggc ggtcggtcgc gtgtcggccg
    12601 cgatcatgcc gtacttcgtg ctgttcgtgc tgaacgggtt cggatcgttt gccgtgttcg
    12661 tgatgctggc gggcgtgctg ctgctattat gcctgctcgt gctcgcggta ggtcccgaaa
    12721 cgcgccgcaa aacgcttgag gatgtcgaac gggaaatcct cgctgacgcc gagctgagcg
    12781 ccgatcacaa gcatgcgaag tcgccggcat cgtgaagatg agacgccgcg ctttgagcgc
    12841 ggcgtgctac aagccgttgg gctatcgcac gtaggcgaac cggcggaata acggggcatc
    12901 ctcgctgcga tgcgtggtga cgcgcgcatg actcatggtg ggatcgagca acgaagccgt
    12961 tctgccctcc atgaccgcgc cgttcatggc gcacaccgga gactgacggc ggccgtcatc
    13021 ggtcgtcagc agccaggaga catcatgaag agaaccgccc gccttcgcgc gttgatcaac
    13081 gcgccgcaaa ttctggccct gcatggggtc tacgacggat tcagcgcgcg cctcgtcgag
    13141 cattgcggat tcacgtccgc cttcatcacc ggcagcggca tcagcgaatc gcgtctcggt
    13201 cagcccgacg ttggcctcat gggtctcgaa gagaacgtcg ctgccgcgcg ctcgctctcg
    13261 gcatgcacca acctcgccct gctcgccgat ggcgacaccg gctacggcaa tgccgtgaat
    13321 gtctatcaca cggtgaaggc tttcgaacag gctggcgtgg ccggcctcat gctcgaagat
    13381 caggtctggc ccaaacgctg tggccatatg aaaggcaagg aagtgatacc gcgcgaggaa
    13441 atggtggaga agatccacgc cgcggtcgcc gctcgggtgg atcccgattt catcatcaag
    13501 tcgcgcacgg atgcgctggc ctcggacggc atggatgaag tgattcggcg gctcaacgct
    13561 tatgcagagg ccggcgcgga cctgctgttc tgtgacgcgt tagtgaccga ggaacagatt
    13621 tcgaccgtgg ccaggaatgt ctccaagccg ctttgcgtga acatgggatt cggtatccgc
    13681 cgacgcgcga caaccccgtt attgtctgcg gcgcatttgc aggatctggg cgtggcggtt
    13741 gtcatttttc cgcggatgct caccgcttgc gcactgatgg gcatgaaaca aggcattcag
    13801 ttgttgcagc agtctttcac gagcgggaac gtaatcgatc gtccggacgt gctggtttcg
    13861 ttcgaggagc tcaacgagat catgggaatc gagcaggtac aggaactcga agcacgcttt
    13921 ctcacgccgg cgcaaaaggc ggccaaatat aagcgcgacg gtgcgcagaa cggcacggtt
    13981 gatgggctat ccgatgatcg atcggctcga aacggagttt ctgctgcctg atggtatcga
    14041 gcgcaaatac ggttcagcgt cggcgagcca ggtggttcgc gagaagcact gaacccgctc
    14101 aacccgctca cgccaccgta tcccatttca cttcctcttc acgcacgatc tgctcgatcg
    14161 tgttctgcac cgcgcgcgac gccgcgttct gcggcaccgc attcggcacg caaagactga
    14221 ccatgcgttc gagcgacggt tccaccagcc gccgccccag caacgccgcg ccgggctggc
    14281 gcgccaacgc accggagagg atcgagccga cctcgccttc gctggcaatc gtcagcatcg
    14341 tgggcagcga atcgatatcg gcgaccacgt tcagcaccag gccttcgcgc gcgaatgtgc
    14401 gctcgatcag aagccgcaac ccgttggcct tgccgggcaa cacgagcggc acgcccgaca
    14461 cgagcgatag cggtacgctg ctgccgttgg cgtcgcccac ccagggcacg ccgcacagcg
    14521 acagatgttc gttgaaaagc ggcgacacgg tgagcccgcg cgtttcggtc tcgcgaaaca
    14581 gcaccgccat gtcgaggcgt ccgttcgcca gcaactcgat cagatagccg ctcatgcttt
    14641 cgaacagttg caactggatg ttgggatacg tgctctgcag acgattgaac agcggcaccg
    14701 cgatcaccgc cgcgatgctg gtcggcagcc ctaccgcgac ttgtcctgac tcgctgtgtt
    14761 cgccgcgccg cacgtcgtcg cgcatctgct cgaactgttt gaggatggtg cgagcgtgcc
    14821 ggtacagggc catgccggct tccgtcggac gcacgccgga gggactgcgc agcagcaaag
    14881 gcacgccgag atcgtcttcc atgccgacga tctgctgact caacgaaggt tgtgaaatcg
    14941 acaggcgcac cgctgctttg gagaggctgc ctgtatcgac gacagtggcg aaataccgta
    15001 gctgacgaac gtccatctga accctcgtga agccggagtc cgggccgcgc gcgaactcgc
    15061 gcgctcgggc tactcgctca ggcggcagga tataagcgcg gactatagca tcaccatgca
    15121 agggttttca gtcgtgcaga cccgcaggaa accccacgcg gtactttgcc ggcgtgccaa
    15181 ggccgacgcc gcgcgcgccg caagcgatgc ttagggccgc gctgccatgg cgagtaaccg
    15241 ctgcgccatg cgcagattgg cgtagtccac catcttgccg tcgaccgata ccgcggcgag
    15301 gcccaccgcc tccgcctgcg ccatggcatc gatcgtcgcc tcggcagcac gaacttctgc
    15361 gtccgtcggt gtgaacgcgg cctgcgtcgc ggcgatctgc tccggatgaa tcacctgctt
    15421 gccgtcgaac cccatcgcgg cggccttgag cgcgaacgaa cgggtcagca ccgggtcgcg
    15481 gaacgcgccg cagggtccgt cgatggcgcg ccgattgaac gcgcgcgccg cgacgaggat
    15541 gcgcatcatc ggatagctcc acagatcgag tggcgcggtg acgtagttgc cgatctcgtc
    15601 cctgcgcgtg accgcatagc ccggatgtgt gccgccgact tcgacattgc gcgcgccaat
    15661 cgatgctgaa aagtcgccga caccgaaatg cagcgcggcc agcctgccgc ccgcggccgc
    15721 gatcgcgtcg acgttcacca tgccgcgtgc ggtttcgatc agcgcctcga tcgcgagttg
    15781 tggatgaacg ccgcgatggt ggtccagtaa agtctcgacc tgctgcagca cacgcgtgga
    15841 ttcgaccttg ggcacgatga tcgcatcgag ccgcgcggcc tcgcccgcga gcgcgctgag
    15901 ttcggattcg agcacgcccg cctgcgtgac attcacgcgc actgcgacat gtttggcgcc
    15961 ccaatcgagt tgcccaagcg cgcgtatggc ggactccagc gccacgctct tttcgctggg
    16021 cgggaccgcg tcttccagat cgatgaagac ggcgtcggcc gcgctcgcgg cggcggattc
    16081 cagcatgcgc cagcggt
//
LOCUS       DATASH010000776         2361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5074196,
            whole genome shotgun sequence.
ACCESSION   DATASH010000776 DATASH010000000
VERSION     DATASH010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5074196"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2216)
                     /locus_tag="VHV99_20805"
     CDS             complement(<1..2216)
                     /locus_tag="VHV99_20805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX3637876.1"
                     /translation="MNISRLFILRPVATLLLMIDLVLVGLVAVRVLPVSSLPNVDYPT
                     IQVQTFYPGASPDVMATTVTAPLEVQLGEIPGLQQMTSYSSDGASVITLQFDLSLNLD
                     IAQQNVQQAINAANSFLPTGLPGPPTYAKVNPADQPILTLAVTSTSMSLTQLEDAANN
                     RLGTKISEVSGVGVVTTSGGNVPAIRVEADPHKLAAYGLNIDDLRTLLSYVNVSQPKG
                     NFDGPDLDYTINGNDQISDPKDYLNTVIAYQNGAPVFMRDVANVTSAPQDVERGAWYN
                     GTPAIVLNVQRQPGANVIATVNQIMKQLPQLESTLPAGMKVTVVSDSTGVIRSSVSDA
                     ALELVLAIVLVVAVIFVFLRNVPATIIPSISVPVSLIGTLAVMYQLNYSIDNLSLMAL
                     IIATGFVVDDSIVMIENIVRYLEEGKTPLEAALEGAGQIGFTILSLTISLIAVLIPLL
                     FMGGVIGRLFSEFAVTLAVTIVLSAVVSLTVVPMLCARLLKAQVDRHPSRFERISEGL
                     FDKTLAAYERGLRWVLDHQILTLMVAVGTVVLTAVLYIVIPKGLFPVQDVGVIEGISV
                     ADNSVSYKAMVTRQRALADAILKDPDVVSLTSYVGIDGTNSTLNNGRFLINLREHDKR
                     SDTAQEIARRLQNEVANVSGIKLYMQPEQDLTLDTTVSPNQYSFVLRGPNQQAFQKYV
                     PELIARLKAIPSLADVQSDMNTDGLSVNVEVNRQLAARFGITPATVDNALYDALGQR"
BASE COUNT          425 a          713 c          783 g          440 t
ORIGIN      
        1 cgctggccca gcgcgtcgta gagcgcgttg tcgacggtgg ccggggtgat gccgaaacgc
       61 gcggccagtt gccggttgac ttcaacgttc acgctcaagc cgtcggtgtt catatcgctt
      121 tgcacatcgg caagcgacgg aatcgctttc aggcgcgcga tcagttccgg cacgtacttc
      181 tggaaggcct gctggttcgg tccgcgcagc acaaaactgt actgattcgg cgagaccgtg
      241 gtgtcgagcg tcaggtcctg ctccggctgc atatagagct tgattccgga cacattggcg
      301 acttcgtttt gcaggcgccg ggcgatctcc tgcgccgtgt ccgagcgctt gtcgtgttcg
      361 cgcaggttga tcaggaagcg gccgttgttc agcgtggagt tggtgccgtc gattcccacg
      421 taagaggtga gcgagaccac atccggatct ttcaggatcg catcggcaag cgctcgctgg
      481 cgtgtgacca tcgccttgta cgagaccgag ttgtcggcca cgctgatccc ctcgatcacg
      541 ccgacgtcct gcaccgggaa caggcccttc ggtatcacga tgtacagaac ggcggtcagc
      601 accacggtgc ctacggccac catcagggtc aggatctgat gatccagcac ccagcgcaag
      661 ccccgttcgt aggcggcgag cgtcttgtcg aagaggcctt cgctgatacg ctcgaagcgg
      721 ctcggatgac ggtcgacctg ggccttcagg aggcgcgcgc agagcatggg caccacggtc
      781 agcgagacca cggcggacag cacgatggtg accgcgagcg tcaccgcgaa ttcgctgaaa
      841 aggcgcccga tcacgccacc catgaagagc agcgggatca gcacggcgat cagcgagatg
      901 gtcagcgaga gaatcgtgaa accgatctgc ccggcgcctt cgagcgctgc ctcaagcggc
      961 gtcttgcctt cctccagata tcgcacgatg ttctcgatca tcacgatcga gtcgtcgacc
     1021 acgaagccgg tggcaatgat cagcgccatc agcgagaggt tgtcgatgga atagttcaac
     1081 tggtacatca cggccagggt gccgatcagg gacaccggca ccgagatgct cgggatgatg
     1141 gtggccggca cgttgcgcag gaacacgaag atcaccgcca ccaccagcac gatggccagc
     1201 acgagctcga gcgccgcgtc ggagaccgac gagcggatca cgcccgtgct gtccgagacg
     1261 accgtgacct tcatgccggc cggcagtgtt gattcgagct ggggcaactg cttcatgatc
     1321 tggttgacgg tcgcaatcac gttcgcgccc ggctgccgct ggacgttcag cacgatcgcg
     1381 ggcgtgccgt tgtaccaggc gccgcgctcg acgtcctggg gcgccgaggt cacgttggcg
     1441 acgtcgcgca tgaatacagg cgcgccgttc tggtaggcga tcaccgtgtt caggtagtcc
     1501 ttcggatcgc ttatctggtc gttgccgttg atcgtgtaat caaggtcagg accgtcgaag
     1561 ttgcccttcg gctggctcac gttgacgtag ctgagcaagg ttcgcagatc gtcgatattc
     1621 aggccatagg ccgctagctt gtgcgggtcc gcttcaacgc ggatggcggg tacattgccg
     1681 ccgctggtgg tgaccacgcc cacgcccgac acttccgaaa tcttcgttcc gaggcggtta
     1741 ttcgcggcgt cttccagttg cgtgagcgac atggaagtgg aggtgaccgc cagcgtcagg
     1801 atcggctggt ccgccggatt caccttcgca taggttggcg gtccgggcag gccagtcggc
     1861 aggaagctgt tggccgcgtt gatggcctgc tgcacattct gctgggcgat gtcgaggttc
     1921 agcgacaggt cgaactgcag cgtgatgacc gaggccccgt ccgaactgta tgaggtcatc
     1981 tgctgcaagc cgggaatctc gcccagttgc acttcgagcg gcgcagtcac cgtggtggcc
     2041 atcacgtccg ggctcgcgcc cggatagaag gtctgcacct ggatggtcgg atagtcgacg
     2101 ttaggcaacg aggaaaccgg cagcacgcgc accgcgacga ggcccactaa tacgaggtcg
     2161 atcatcagca gcagcgtggc taccggtcta agaataaaca ggcgggagat attcatcggc
     2221 gcgtggggtc ggctctacga ggctgcgtcg gccgcggcgg cagccgcgcc cgaggcaccg
     2281 gcgtgggcgt gcttatgctg accctgagcg ccgctcgctc cggacgccgg ggcttcggcg
     2341 gcagcgccgc ccgaggcggg t
//
LOCUS       DATASH010000777         7703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5269938,
            whole genome shotgun sequence.
ACCESSION   DATASH010000777 DATASH010000000
VERSION     DATASH010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7703
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5269938"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..1156
                     /locus_tag="VHV99_20810"
     CDS             47..1156
                     /locus_tag="VHV99_20810"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX3637877.1"
                     /translation="MDISKPQQNASAPDLATRDASAQTYAEDAPNSAIDDALARILGN
                     PPAAECPIMVLLVDDQAIIAEAIRQALADEESVDFHYCASPEEAVHCAAETRATVILQ
                     DLVMPGTDGLTLVRQYRQNPATRDIPIIVLSTKEEPLVKSAAFAAGANDYLVKLPDRI
                     ELIARIRYHSRSYLNLLQRDEAYRALRQSQQQLLATNLELQRLTHSDGLTGLSNRRYL
                     DQYLAAEWRRGTRDKTGLGFLMIDVDNFKAYNDTYGHVAGDEVLKQVAHTVESCLGRS
                     SDLAARFGGEEFAVVLPGASSGGLRLLAEKIRIAIEALDIPHAGSASGVVTISIGAAM
                     IVPSGSEPVTTLIEAADVGLYRAKRDGKNQVAVSG"
     gene            complement(1262..4063)
                     /gene="mdeB"
                     /locus_tag="VHV99_20815"
     CDS             complement(1262..4063)
                     /gene="mdeB"
                     /locus_tag="VHV99_20815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate dehydrogenase"
                     /protein_id="HEX3637878.1"
                     /translation="MTDLSSGSEQLQSLDLQRAERAEREDTDPQETVEWLEALDAVVE
                     HIGRDRAQFLFDKLAEHALSLGVESARTNVTPYQNTIPFEQQPRYPGNLELEERLAGA
                     LRWNALAMVVRANQAYGELGGHIASYASAADLFEVGFNHFFRAAAPSGEEGTGDLVYF
                     QPHSSPGVYARAYLEGFLTEQHLRHYRREIGGPGLCSYPHPWLMPDFWQFPTGSMGIG
                     PINAIYQARFMRYLANRGLAQTDGRKVWGFFGDGEMDEPESTGALSLAAREGLDNLVF
                     VINCNRQRLDGPVRSNGRIIDELEAHFIGAGWNVIKVVWGSDWDALFSRDRTGALLRA
                     FAHTVDGQFQTFSANDGAYNRERFFGQNPELAALAAQLSDDDIDRLRRGGHDVRKLHA
                     AYAKALAHRGQPTVILAKTMKGFGMGTTGQGRMTTHQQKKLGFGDLKAFRDRFRLPLT
                     DEDVEQVKFYKPADDSPEMQYLHARRAALGGYLPRRRSVASKGLIVPPMPSWGQFALD
                     SNGREMSTTMALVRMLTALLKDREVGPRVVPIVADEARTFGMANMFRQVGIYSPLGQL
                     YEPEDLGSMLYYREDTKGQILEEGISEAGAVSSWIAAATSYSVHNLPMLPFYIYYSMF
                     GFQRIGDLIWAAADQRARGFLIGATSGKTTLGGEGLQHQDGTSHLAASTIPNCRAYDP
                     AFAYEVAAIVDEGMREMVEQQRDVFYYVTVMNENYAQPSVPGGDLQTLREGILKGIYR
                     LATEVQETASATQTQDATSTSTRHVRKATSGQVQLLVAGAILGEVIAAQQLLKDEWQI
                     DAAVWSVTSFTELQRDGMTSERNARLGEATETPYVTKVLEASHGPIIAATDYVRAVPE
                     LIRAYMPRRYVTLGTDGFGRSDTRQALREFFEVDRKAIVIAALKALVDEGAIDASVLA
                     KARSKYRGDAPAGAASWQC"
     gene            4216..4743
                     /locus_tag="VHV99_20820"
     CDS             4216..4743
                     /locus_tag="VHV99_20820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX3637879.1"
                     /translation="MTTPPRRLDRIDIGILSQLQQNARITNADLARAVNLSPTPCFNR
                     VRALEKLGLFKQQVTLLNPEPLGLRINVFIQVSLEKQVKDALLRFEQAISERPEVMEC
                     YLMTGDADYLLRVVMPDMQTLERFIVDHLTKIPGISNIRSSFALKQVRYKTALPLPSS
                     GLTLVDPDDVATDWR"
     gene            complement(4861..5673)
                     /locus_tag="VHV99_20825"
     CDS             complement(4861..5673)
                     /locus_tag="VHV99_20825"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3637880.1"
                     /translation="MATQGNKGTALITGASSGIGAVYADRLARRGYDLILVARDVKRL
                     NGVAERLASETGRHIDIVAADLTDKADTRRIEERLRTDRNITMLVNNAGVGATASLID
                     SDVDHLELMIDLNVTALTRLTAAIVPGFVERGNGVLINISSVVALSPELLNGTYSGTK
                     AYVLNLTQSLHHEVGGKGVQLQAVLPGATSTAFWDRAGRAVEHLPTQIVMTAEDMVDA
                     ALVGLDQGELVTIPSLPDAADWERFNTARQHLGPNLSHKLPAARYTHGAAAA"
     gene            5850..6785
                     /locus_tag="VHV99_20830"
     CDS             5850..6785
                     /locus_tag="VHV99_20830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HEX3637881.1"
                     /translation="MKRVGVVVFPGFQILDMVAISVFELANLEAGQPEYAVEVISEQG
                     GMVRSSSGVEIATQPFGDPAYDTVVVTGSMQIEPSSPALLAFLNDALAASRRTTSICT
                     GAFVLAEAGILDGRHATTHWHHARELQRRFPETRIDDDRIFIVDGSVWTSAGMTACID
                     MCLALIESDLGVAVSRAIAKKLVVYHRRTGGQSQFSAMLDLEPKSDRIQDALSYAKSH
                     LREPLTVEQLADVAHLSPRQFSRAFRDETRQSPAKAIEALRVEAARTMLEAGRHSMEA
                     VAAETGFIDTERMRRAFLRAFGQPPQAIKRAARAL"
     gene            6856..7281
                     /locus_tag="VHV99_20835"
     CDS             6856..7281
                     /locus_tag="VHV99_20835"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEX3637882.1"
                     /translation="MNYDDSNPFAKILRGELPCIKVAESDAALAFMDLMPQADGHVLV
                     VPKEAAVEIFELSDASTVACMRMAQKLAIAIRAALRPDGVFIGQFNGAAAGQTVPHVH
                     FHVIPRWEGQPLRMHARELADAATLEALAKRIRDHWRAH"
     gene            complement(7288..7464)
                     /locus_tag="VHV99_20840"
     CDS             complement(7288..7464)
                     /locus_tag="VHV99_20840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637883.1"
                     /translation="MIEHLILGAFLVIPLLIVAFLFSDELWQEHRHLHDIDLEPVKPR
                     RDWRHPLRRWRHRL"
BASE COUNT         1425 a         2490 c         2364 g         1424 t
ORIGIN      
        1 acattagctc gctacgattc actgaaattt attctggctg accaatatgg acatttccaa
       61 accgcaacag aacgccagcg cgccggatct cgccacacgc gacgcgagcg cgcagacgta
      121 cgcggaggac gcgcctaata gcgccatcga cgacgcgttg gcgcgtatcc tcggcaatcc
      181 gcccgcggcc gagtgcccga tcatggtgct gctggtcgac gatcaggcga tcatcgccga
      241 ggcgatccgt caggcgctcg ccgatgaaga gagcgtcgat ttccactact gcgcgtcgcc
      301 cgaagaagcg gtgcactgcg cggcggaaac ccgggcgacg gtgatcctgc aagacctcgt
      361 gatgcccggc accgacggcc tcacgctcgt gcggcaatac cggcagaatc cggcgacgcg
      421 cgatattccg atcatcgtgc tgtcgaccaa ggaagagccg ctggtgaaaa gcgcggcctt
      481 cgctgcgggc gcgaacgact atctggtgaa gctgccagac cgcatcgagc tgatcgcgcg
      541 gattcgctat cactcacgct cgtatctgaa tctgctgcag cgtgacgagg cttaccgagc
      601 gctgcgtcaa tcgcaacagc agttgctcgc aaccaatctg gaattgcagc ggctcacgca
      661 ttcggacggc ttgacgggct tgtcgaaccg ccgctatctc gatcagtacc tcgccgctga
      721 atggcgtcgc gggacgcgcg acaagaccgg cctcggcttc ctgatgatcg acgtggataa
      781 cttcaaggcc tacaacgaca cctatgggca cgtcgccggc gacgaagtgc ttaagcaggt
      841 cgcgcacacc gtcgagtcgt gcctgggacg ttcgtccgat ctcgcggcgc ggtttggcgg
      901 cgaagagttc gcggtggtgt tgccgggggc atcctcgggt ggcttgcggc tgctggctga
      961 aaaaattcgc attgctatcg aggcactcga cattccgcat gcaggctcgg cgagcggtgt
     1021 cgtgacgatc agcatcggtg cggcgatgat cgtgccgtcc ggctcggagc cagtgacgac
     1081 actgatcgaa gccgccgacg tcgggctgta tcgagcgaag cgggatggga agaatcaggt
     1141 ggcggtgagc ggctgatttt tttgcgcggc ggtttgagcg ctgccaagtg gcaacgcgcg
     1201 tcggcatgtc gccgaagcgc atttttaatc gaagcggcgc gggttcatta gcggcgcggc
     1261 gtcagcattg ccacgacgca gcacctgctg gcgcatcgcc gcgatacttg ctgcgcgctt
     1321 ttgcgagcac gcttgcatca atagcgccct cgtccaccag cgccttcaac gccgcgatca
     1381 caatcgcctt gcgatccact tcgaagaact cgcgcaacgc ctggcgtgtg tcgctacggc
     1441 cgaagccatc ggtgccgagc gtcacgtagc ggcgcggcat gtaggcgcgg atcagttccg
     1501 gcaccgcgcg gacgtagtcg gtcgcggcga tgatcgggcc gtgcgaggct tcgagcactt
     1561 tcgtcacgta tggcgtttct gtcgcctcac caagacgcgc gtttctctcc gacgtcatcc
     1621 catcgcgctg caattcggtg aagctcgtga cactccagac ggcggcgtcg atctgccatt
     1681 catccttgag cagttgctgc gcagcgatga cttcaccgag gatcgcgcct gccacgagca
     1741 actgcacttg ccctgaagtt gccttccgta cgtgcctcgt tgatgttgag gttgcgtcct
     1801 gcgtttgcgt cgccgaggcc gtctcctgca cttccgtggc gagccgataa atacccttaa
     1861 gaatgccctc gcgcagcgtc tgcaaatcac ctcccggcac cgaaggctgc gcgtagttct
     1921 cgttcatcac cgtcacgtaa tagaacacgt cgcgctgttg ttcgaccatc tcgcgcatgc
     1981 cctcgtcgac gatcgcggcg acttcatagg cgaacgccgg atcgtaggcg cgacagttcg
     2041 gaatcgtcga tgccgccaga tggctcgtgc cgtcctgatg ctgcaaccct tcgccaccaa
     2101 gcgtcgtctt gcccgacgtc gcgccgatca ggaaaccgcg cgcgcgttga tccgccgccg
     2161 cccagatcag atcgccgatg cgctgaaagc cgaacatcga gtaatagata tagaacggca
     2221 gcatcggcag gttatgcacg ctgtacgaag tcgccgctgc aatccaggac gacaccgcgc
     2281 ccgcctccga aatgccctct tcgagaatct ggcctttcgt atcctcgcgg tagtacagca
     2341 tcgagcccag gtcttccggc tcatacaact gcccaagcgg cgaatagatg ccgacctggc
     2401 ggaacatgtt cgccatgccg aaggtgcgtg cttcatcggc gacgatcggc accacgcgcg
     2461 ggccgacttc gcggtccttg agcagcgcgg tcagcatgcg gaccagcgcc atcgtcgtgg
     2521 acatctcgcg gccattcgaa tccagtgcga attgccccca ggagggcatc ggcgggacga
     2581 tcagcccctt cgacgccacg ctccgccttc tgggcagata gcctcccagg gcagcccggc
     2641 gcgcatgcag gtattgcatc tccgggctgt catccgcggg tttgtaaaac ttcacctgtt
     2701 cgacgtcctc atccgtcagc ggcaaacgga agcggtcgcg aaacgccttc agatcgccga
     2761 agccgagttt cttctgctga tgcgtggtca tccggccctg gcctgtcgtg cccatgccga
     2821 agcccttcat cgtcttcgcg aggatcaccg ttggctggcc gcgatgcgcg agcgctttcg
     2881 catacgccgc gtgcagcttg cgcacgtcgt ggccgccgcg gcgcaggcgg tcgatgtcgt
     2941 cgtcggagag ttgcgcggcg agcgcggcaa gctccgggtt ctggccgaag aaacgctcgc
     3001 ggttgtacgc gccgtcgttg gcggagaagg tctggaactg gccgtcgacg gtgtgcgcga
     3061 acgcgcgcaa caacgcgccg gtacggtcgc gcgaaaacaa cgcgtcccag tccgagcccc
     3121 acaccacctt gattacgttc cagccggcgc cgatgaaatg cgcttcgagt tcgtcgatga
     3181 tgcggccgtt gctgcgcacc ggaccatcca ggcgctgccg attgcagttg atcacgaaca
     3241 cgaggttatc gagcccctcg cgcgccgcga gcgacagcgc gccggtcgat tccggctcgt
     3301 ccatttcgcc gtcgccgaag aaaccccaca ccttgcggcc atcggtctgc gcgaggccac
     3361 ggttggcgag gtagcgcatg aagcgcgcct ggtagatcgc gttgatcggg ccgatgccca
     3421 tcgagccggt cggaaactgc cagaagtccg gcatcagcca cggatgcgga tacgaacaca
     3481 gccccggccc gccgatttcg cgacggtaat gccgcagatg ctgctcggtc aaaaagcctt
     3541 cgaggtaagc ccgcgcgtac acaccgggcg acgaatgcgg ctggaagtac acgagatcgc
     3601 ccgtgccctc ctcgccgctc ggcgccgcag cgcggaagaa atgattgaag ccgacctcga
     3661 acagatccgc cgccgacgca taactcgcaa tgtgaccacc gagttcgccg taagcctggt
     3721 ttgcacgcac caccatcgcc agcgcattcc agcgcagcgc gccggcaagg cgctcttcaa
     3781 gctcgagatt gcccggataa cgaggctgct gctcgaacgg gatcgtgttc tggtaaggcg
     3841 tgacgttggt ccgcgccgac tcgacaccca gcgacagcgc atgctccgcg agcttgtcga
     3901 acagaaactg cgcgcgatca cggccgatat gctcgacgac tgcatcgagc gcttccagcc
     3961 attcgacggt ttcctgcggg tcggtatctt cgcgctcagc acgttcagcg cgctggagat
     4021 ccaaagactg caactgctcg ctaccactgg acaaatccgt catggcacca ctcccgtatc
     4081 cacacgtccg gctcaagatc gagtgagtca atggtaccca cgtggtcaca aaattagcct
     4141 ctcatttgct tgtcatcgcg cgctgcgata ggctatttgt tccgaatttc acgcctgtca
     4201 cggaatattt catctatgac cacgcccccc aggcgactcg accgcatcga catcggcatc
     4261 ctgagccagt tgcagcagaa cgcgcgtatc accaatgcgg atctcgcccg cgcagtgaac
     4321 ctgtcgccga cgccctgctt caatcgcgtg cgtgcgctcg aaaagctggg tctgttcaag
     4381 cagcaggtca cgttgctgaa ccccgagccg ctcgggctgc gcatcaacgt gttcattcag
     4441 gtgagcttgg aaaagcaggt gaaagacgcg ctgctgcgtt tcgagcaggc gatttcggag
     4501 cgccccgagg tgatggagtg ctatctgatg acgggcgacg ccgactatct gctgcgcgtg
     4561 gtgatgccgg atatgcagac gctggagcgc ttcatcgtcg accatctgac gaagattccg
     4621 ggcatttcca acattcgttc gagctttgcg ctcaagcagg tgcgctacaa gacggcgttg
     4681 ccgctgccgt cgtcgggact gacgctggtc gatcccgacg acgtggcgac ggattggcgg
     4741 tgagcgtccg gcgcgccgca ctatgcctct gacccacacg tctagcgcgc aatgggcaac
     4801 gtatgcgacg gatgctgcca cgcggcgttg aggcgcgctc aatcagccgc gtcagacgca
     4861 ctaagccgcc gctgccccgt gcgtataacg cgcagccggc agcttatgcg acagattcgg
     4921 ccccagatgc tggcgagcgg tgttgaagcg ctcccaatcg gccgcatccg gcagcgacgg
     4981 aatcgtcacc agttcgcctt gatccaggcc gacgagcgca gcgtccacca tgtcctcggc
     5041 ggtcatcacg atctgcgtgg gcagatgctc gaccgcccgt cccgcgcggt cccagaacgc
     5101 cgtgctggtg gcacccggca gcacggcctg caactgcacg cccttgccgc ccacttcatg
     5161 atgcagcgat tgcgtcaggt tcaacacgta ggccttggtg ccgctgtagg tgccgttcag
     5221 caattccggc gacagcgcga cgaccgacga aatattgatc aggacaccgt tgccgcgttc
     5281 cacgaaaccc ggcacgatcg ccgccgtcag gcgcgtcagc gccgtcacgt tcaggtcgat
     5341 catgagttcg aggtgatcca cgtccgagtc gatcagcgaa gccgtcgcgc cgacgcccgc
     5401 gttgttgacc agcatcgtga tgttgcggtc ggtccgcaga cgttcttcga tgcggcgcgt
     5461 gtcggctttg tcggtgagat cggcggccac gatatcgatg tgccggccgg tttcgctggc
     5521 gaggcgctcg gctacgccgt tgaggcgctt gacgtcgcgc gccaccagaa tcaggtcgta
     5581 accgcgccgc gcgaggcggt ccgcatacac cgcgccgatg cccgacgatg cgcccgtgat
     5641 caatgccgtg cccttgtttc cttgcgttgc catgtgaagc tctccttgcg ggctgtgcgc
     5701 gtcacgcctg agccctgatc gatgaatggg gaaaacctgg tgccaccgcc gccctgccct
     5761 ctgattggcg acgcggtgtt gacgagtgaa gtctaggcgc gtagtctttt gtctcaaatg
     5821 tcgtatttac ctcgttttgg gacatcgcga tgaagcgcgt tggcgtggtg gttttccccg
     5881 gcttccagat cctcgatatg gtggcgatct ccgtgttcga gctggcgaac cttgaggccg
     5941 gtcagccgga gtacgcggtg gaagtcattt ccgaacaggg cgggatggtg cgcagttcgt
     6001 cgggcgtcga gatcgcaacg caaccgttcg gcgatccggc ctatgacacg gtggtggtga
     6061 cgggctcgat gcagatcgag ccgtcatcgc cggcgttgct ggcgtttttg aacgacgcgc
     6121 tcgccgcctc gcgccgcacc accagcatct gcaccggcgc cttcgtgctg gccgaagccg
     6181 gcattctgga cggccggcac gccacgaccc actggcacca cgcccgcgag ttgcagcggc
     6241 gctttcccga gacccgtatc gacgacgatc gcattttcat cgtcgacggc tcggtgtgga
     6301 cctcggccgg catgacggcg tgcatcgaca tgtgtctggc gctgatcgaa agcgatctcg
     6361 gcgtcgcggt ctcgcgcgcc atcgccaaga agctcgtcgt ctatcaccgg cgcaccggcg
     6421 ggcagtcgca gttctcggcc atgctcgatc tggagccgaa gtccgaccgc attcaggacg
     6481 cgctgtcgta cgccaaaagc catctgcgcg agccgttgac ggtcgaacag ctcgccgacg
     6541 tcgcgcatct gagcccacgt cagttcagcc gcgcctttcg cgacgaaacg cgccaatcgc
     6601 cggccaaggc catcgaggcg ctgcgcgtgg aggccgcccg caccatgctc gaagcgggcc
     6661 gtcactcgat ggaagcggtc gccgccgaaa ccggcttcat cgataccgaa cggatgcggc
     6721 gcgcctttct acgcgcgttc ggccagccgc cgcaagcaat caaacgcgca gcacgcgctt
     6781 tgtaatagat gtagtggaca atattgccga cagcagctca cctttattac cgacggcaac
     6841 ctacgaggac gcgtgatgaa ctacgacgac agcaatccct tcgcgaaaat tctgcgcggc
     6901 gaactaccct gcatcaaagt ggcggaaagc gatgcggcgc tggccttcat ggacctgatg
     6961 ccgcaagccg acgggcacgt gctggtcgtg ccgaaggaag cagcggttga gatattcgag
     7021 ttgtcggatg cgtcgacagt ggcctgcatg cgtatggcgc agaagctcgc catcgccatt
     7081 cgcgcggccc tgcgcccgga cggcgtgttc atcggacaat tcaatggcgc cgcagcggga
     7141 caaacggtgc cgcacgtgca ttttcacgtg atcccgcgct gggaaggaca accgctacgc
     7201 atgcatgcgc gcgaactcgc cgatgcggcc acgctcgaag cgctggccaa gcgcattcgc
     7261 gaccattggc gcgcccactg acgctattta cagccgatgc cgccagcggc gcagcggatg
     7321 ccgccagtcg cggcgaggtt tcacgggctc gagatcgatg tcgtgcagat gccgatgctc
     7381 ctgccacaat tcgtcggaaa aaagaaacgc gacgatgagc agcgggatga ccagaaaggc
     7441 gcctaatatc aagtgctcga tcacggtagc ctccatcgca atagcatgat ttcattgtag
     7501 agcaccgtat ggacccgcac ggtgccaagc ccctcacaaa atcgcgataa ccggcctgcg
     7561 acaacgcgac gcccctccct tgccgaaccc gcgcagcgtc tcgcgcttcg caagaacgcg
     7621 ctgcgcgggc gcgaggcgtt gtaagaactc cctgcgctga ggcctgacta acaggaaacg
     7681 caagcctgag ctctcacgct gag
//
LOCUS       DATASH010000778        10978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2502295,
            whole genome shotgun sequence.
ACCESSION   DATASH010000778 DATASH010000000
VERSION     DATASH010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 10978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10978
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2502295"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1561)
                     /locus_tag="VHV99_20845"
     CDS             complement(<1..1561)
                     /locus_tag="VHV99_20845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3637884.1"
                     /translation="MKLFTKGLLLIAVPSVVELALLGVVFDTQEQTAQAAQWVSNSKQ
                     ILYQSSAIVDPLLRQAARVRTSMVVGDPSFIDRHTVWVDLGDRLSNLAVLVADTPQQV
                     ERVRKMQQAIDTYRAQAATVSQALHAGRSLNSFASLDPGALPRQIALFRNELADFTNE
                     ASRLDAERGAALARRRERQQYALIAAVLGSMLIWAATAIVFARSIGRRLEVLTDNAER
                     LGNGRQLAAPLSGSDEIAALDAVLHQTGARLREAESEQALLKARLEARAGELAGVNEE
                     LRQETQDNEMFIYSVSHDLRSPLVNLQGFSKELQVSCDELDSIVAAAKLPEAEHRRMV
                     HILDGDVRESLQYLRTAVTQAASIIDALLRISRAGRLEYQWQRVSVGRVVGRVVDALQ
                     SAIGQREAVVTVRELPPAWGDPGAIEQIFSNLIGNAINYLDPARHGRIEIGALEPELE
                     AFDEREPRALRTRTYYVRDNGLGIPAAYMSKVFRAFQRLHGDVANGDGIGLAVVRRMV
                     ERHGGRVWVESA"
     gene            1963..2457
                     /locus_tag="VHV99_20850"
     CDS             1963..2457
                     /locus_tag="VHV99_20850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637885.1"
                     /translation="MRTLRFLQFFFRRIFMSFPKKRRRAKVDGDESFLAVLRHFKPFG
                     QLDTKIARARAQDEPVLYAHVLPGLDVLLCSVRGAQPPYPAPAELRRRCIESITNALE
                     QPLDGLENGGYWYEADGFGFLVFASRARGKILTEFGATRVGGARRGVRRQDAAGDAAP
                     RSLR"
     gene            complement(2714..5206)
                     /locus_tag="VHV99_20855"
     CDS             complement(2714..5206)
                     /locus_tag="VHV99_20855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEX3637886.1"
                     /translation="MAVSSTIGRRIGKIIAWLVAIIVVLIIALIIFILTFNWNRARPY
                     IDDKVTQAIGRPFAINGDLKVGWRHPVGETGWRGWVPWPRFSAANITIGNPDWTQQPH
                     FATLDEIDFEVKVLPLLAHDIVIPAINLVNPSVDLERLLDGRNNWTFKLPASTGPSEW
                     KLDLHDIAFAKGNIALSDQQKKVEMQIAIDTLGQSIPIGEAMKQQEAASRSASAEAIG
                     KAGANKLTAQANAQAVSEAAAASAAAASGASSTDITASGATAANGGSGALVAGGSSGS
                     SGEGGASAGAGGAAVTSASNASEAGTANSAKPVIPPYAIGWTVKGTYNKTPVLGSGKV
                     GGVLALQDANRPFPVQADVKAGDLHVGLVGTITDPAHLAAVDLRLWLQGNSLARLYSL
                     TGVTLPDTPPYATEGRLVGQFKSSGNVFKYENFTGRVGGSDLNGSLIYTAREPRPLLQ
                     GELVSHLLQFSDLAPIIGADSNASKAKRGDATAQPGNKALPVEEFRTDRWKAIDADVK
                     FTGRRIVKSPDLPITDLYTHVVMTDGVLSLEPLKFGVAGGALSSNIHLDGSTTPLKGR
                     FATSARHLKLKQLFPNFKTMQNALGEINGDAALTATGNSPAALAATSNGEVKALVTDG
                     TVSRLLMEAAGLNVANVVYEKLFGKRDVKINCAAADFVATNGVLDSRVFALDTDDAVI
                     DIDGNVNMRDETMDLGVHPHTKGFRVFSLRSPLYVKGTFKDPHVGVNAAALALRGGAA
                     VGLGLINPFAALIPLLAPSNNKPLPCAQLLSQVRQAPSAPPPGVKQQPKAAISLDGVP
                     VNKSSGGASAPAASEKKPAVVTPASAAEYKGS"
     gene            5418..6128
                     /locus_tag="VHV99_20860"
     CDS             5418..6128
                     /locus_tag="VHV99_20860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3637887.1"
                     /translation="MTDVPFVLAEGIARRDALRGQTLLQPTTFALRAGDRVAVTGPSG
                     SGKSVFLRTLALLDPLDAGRIMWHGAPVERAAIPRYRRNVAYIRQRPALLDGSVEDNL
                     RYPFELRTYRDVRFDRARAAKLAAQAGRTDDFLDKRASELSGGEAQITALIRVLQLAP
                     EVLLLDEPTASLDPESSHAIEGLVHAWFDAEPGRHASIWVSHDPAQAARMSERHLTMR
                     AGVLDESAATPAPQEFDR"
     gene            6125..6931
                     /gene="fetB"
                     /locus_tag="VHV99_20865"
     CDS             6125..6931
                     /gene="fetB"
                     /locus_tag="VHV99_20865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HEX3637888.1"
                     /translation="MTLQNLSLWDVAIAALLIVVNGVVSVALKLDLERKLAWAAVRTV
                     VQLLAIGYVLGWVFRYDHWFVVLPLMIVMTLIAGFAGAQRGSRTYAGQRADSVLSIWV
                     SSWLVAAVGLFVVIRIHPWYEPQYAIPILGMILGNTLTGVSLGIERMTEELTARRDRV
                     DMALALGATRWEAAQAPARQAVRAGMMPTLNQMAVVGVVSLPGMMTGQVLAGQSPLQA
                     VRYQIVIMFLIAAASALGTVGAVLLTYRRLFSAEHRFLAARLVERTAARR"
     gene            complement(7126..7689)
                     /locus_tag="VHV99_20870"
     CDS             complement(7126..7689)
                     /locus_tag="VHV99_20870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6013 family protein"
                     /protein_id="HEX3637889.1"
                     /translation="MSSSFKLSAVSVVASLACALAPGMFAPAAHAATPITVTSQSALD
                     GPIRYTVRVTSKQFGNSQETRTIRSGESDDFTWKTVPPGGPVPATDSCPNYASLPVDT
                     NGAMIRQTQIRFAPVVGGDGTASVQLSFQAQTPHGVKTVTSGGKTIKCPNDVSVSQIL
                     RFTMPTNGTTKTLTLNDGTEVAVTAKR"
     gene            complement(7953..8969)
                     /locus_tag="VHV99_20875"
     CDS             complement(7953..8969)
                     /locus_tag="VHV99_20875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEX3637890.1"
                     /translation="MKAVGLYRYLPIDQAESLVDVEIPKPEATGRDLLVKVEAISVNP
                     VDTKVRAPKDTVEKAPRVLGWDAAGTVVAVGPDVTLFKVGDPVFYAGSITRPGANSEF
                     HLVDERIVGRKPASFDFTHAAALPLTAITAWEALFDRLGVSPQGADEGRTVLIVGGAG
                     GVGSIGIQLAKQLAKLKVIATASRPESAKWATELGADHIVDHFDDMPAQLKKLGIDQV
                     DYVLIFNDTDKNFPAAAEVIKPQGRICSIVENRQPVPVELLKAKSAAFHWEFMFTRSM
                     FGTPDMIEQHKLLTEVARLVDAGTLCTTVGQNLGKINAANLRRAHQLLEEGRAIGKLV
                     LTGF"
     gene            complement(9281..9583)
                     /locus_tag="VHV99_20880"
     CDS             complement(9281..9583)
                     /locus_tag="VHV99_20880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637891.1"
                     /translation="MKASPQSSDGGKQADPHAKKHPRSMQEASAPLPHETDQDPESQH
                     EDNPRHVGKQAHSDVERGLKDTDRRGGDEYQQETQNDAHTDVNSDGKAGGGGRGKR"
     gene            complement(9628..>10978)
                     /gene="ligD"
                     /locus_tag="VHV99_20885"
     CDS             complement(9628..>10978)
                     /gene="ligD"
                     /locus_tag="VHV99_20885"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HEX3637892.1"
                     /translation="AHETKRMPFASAPRERSRTPVHWVEPVLVCECNFAEWTSDGIVR
                     QASFVSLRDDKPARQIVKEAPRKGADVQEQTDTASEAVPKKRTAKTTAASAAAEPART
                     SAAKPSTTKAAATKISAAKTSAKSTPAAKAAATRTPATKATAAKSKTAATPAEVAGVR
                     VSHPDRIIDKSTGTRKIDLVQYYESVADWMLPHLQERPVSLVRAPEDLGGELFFQKHS
                     QKLSIPNVTQHPGLDPGHPPLITVDTLKALVGAAQMGTIEFHTWNAVASNIEKPDRVV
                     FDLDPDASLGWERMIEAAQLTHSLLEELGLTSFCKTSGGKGFHVVVPLAKQAGWDEVK
                     AFSQAVAQHMAATLPKYFSAKMGAQNRKQKIFVDYLRNNRGSSTVAAFSARARPGLGV
                     SVPLAWDEVATTTSGDQWTIENVHERLEDLKRDPWADYAKTRQRITTAMKKRLDDAQ"
BASE COUNT         1797 a         3573 c         3468 g         2140 t
ORIGIN      
        1 ccgccgactc gacccacacg cggccgccat gccgttccac catccgccgc accacagcga
       61 ggccgatgcc gtcgccgttc gcgacatcgc cgtgcagacg ctgaaacgcc cggaatactt
      121 tcgacatata cgccgccgga atgccgaggc cgttgtcgcg cacataatag gtgcgcgtgc
      181 gcaacgcgcg tggctccctc tcgtcgaacg cttccagctc cggctccaat gcgccgatct
      241 cgatgcgccc atggcgcgcc gggtcgagat aattgatcgc gttgccgatc aggttgctga
      301 aaatctgttc gatcgcgccc ggatcgcccc atgcgggcgg caattcgcgc acggtgacca
      361 ccgcttcgcg ctgcccgatc gcactctgca gcgcatcgac cacgcggccc actacccgtc
      421 cgacactcac ccgctgccac tggtattcga gccggccggc ccgcgagatc cgcaataacg
      481 cgtcgatgat cgacgccgcc tgcgtcaccg cggtgcgcaa atattgcagc gattcgcgca
      541 cgtcgccgtc gaggatatgc accatacgcc ggtgctcggc ctccggcagt ttcgccgccg
      601 cgacgatgct gtccagttcg tcgcacgaca cctgcaattc tttggaaaag ccctgcagat
      661 tcaccaacgg cgagcgcaga tcgtgcgaca cgctgtagat gaacatttcg ttgtcttgcg
      721 tctcctggcg cagctcctcg ttgacgcccg ccagttcccc cgcgcgcgct tcgagccgtg
      781 ctttcagcag ggcctgctcg ctttcggcct cacgcaggcg cgcgccggtc tggtgcagca
      841 ccgcgtcgag cgcggcgatt tcgtcgctgc cggagagcgg cgccgcgagt tgccggccat
      901 tgcccagacg ttccgcatta tccgtcaaca cctccagccg ccgcccaata ctgcgcgcaa
      961 acactatggc ggtggccgcc cagatcagca tcgaacccaa caccgcggcg atcagcgcgt
     1021 attgctggcg ttcgcggcgc cgtgccaggg ccgcgccgcg ctcggcgtcg agccgcgaag
     1081 cctcgttagt aaaatcagcg agctcgttgc gaaacagcgc gatctgccgc gggagcgcgc
     1141 cggggtccag cgaggcaaac gaattcaggc tgcgcccggc gtgcagcgcc tgcgagaccg
     1201 tggcggcctg cgcgcgatac gtatcgatcg cctgctgcat cttgcgcacg cgctcgacct
     1261 gttgcggcgt atcggccacc agtaccgcaa gattcgaaag ccggtcgccg agatcgaccc
     1321 atacggtgtg gcggtcgata aacgacggat cgccgaccac catgctcgtg cggacccgcg
     1381 ccgcctgacg cagcagcgga tcgacgatag ccgacgactg ataaaggatc tgtttgctgt
     1441 tgctgaccca ctgcgcggcc tgcgctgtct gctcctgggt atcgaagacg acgcccagaa
     1501 gcgccagctc gaccacgctc ggcactgcaa tcagcagcag acccttggtg aatagtttca
     1561 tgttcgcccg cagggtcgcg aagggtcact catggcgcgg cggcgcgccc ggaccgcgta
     1621 ccccggcgtg tgggcggccc ctcgcggtgc cggattggcg agtccgccgc gccgcgaccg
     1681 gacgggccat tatcgacgtg aacgtgagat atttcaacgt agcggcaaga tgcgctcgcc
     1741 cacacaaaaa tgactttttt attggtatga gatgtttttt cggtggcgcg tgcgtctatg
     1801 atggaaacag atgtgctggg aagcgaaagc gcgcggcgag gcgattcggc atgatttgtg
     1861 cgggaggcgg tgggacggcg cagtcgccga atcaccgtga atccggccct gtcgtatgct
     1921 ataaaagatc gacgtgcggc gcgcgaagcc gggagttgtc gcatgaggac tctgcgtttc
     1981 ttgcaattct ttttcaggcg aatttttatg tccttcccga aaaagcgtag acgcgcgaag
     2041 gtggacggcg atgagtcgtt cctcgcggta ctacggcatt tcaagccgtt cggccaactc
     2101 gacaccaaaa tcgcgcgcgc tcgcgcgcaa gacgagccgg tgctctatgc gcatgtgctg
     2161 cccgggctcg acgttctcct gtgcagcgta cgaggcgcac agccgcccta tcccgcgccc
     2221 gccgaattgc ggcggcgttg catcgaatcc attaccaatg cgttggaaca gccgctcgat
     2281 gggctcgaga acggcgggta ctggtatgag gccgacggat ttgggttcct ggtctttgcg
     2341 agccgcgctc gcggcaagat cctcacggaa tttggcgcga cccgagtggg gggcgcccgc
     2401 cgcggggttc gcaggcagga tgctgcggga gacgccgcgc cgcgctcgtt gaggtaagtc
     2461 cttttctttt tccttcctct ggcagcgccg ttgtccgtac gtccgcggcg ctgatcctct
     2521 tctgtcgttt cccgttcgcc tgcgcagtgc tgcgatggtt tgcccacgcc cctacggcat
     2581 cgcctttctt ttttcccgct gtgcagtcag gattcgcccc gtggcggggg aacgctaatc
     2641 agccacaaga aaatcaggga aagggctgcg ccgaaggtcc aactccatca atccgcaggg
     2701 aaggcaaaaa acatcagctc cccttatatt cggccgcact ggccggcgtc acgacagcgg
     2761 gtttcttttc ggacgcagcc ggtgcggaag cgccgcccga agatttgttc acgggcacgc
     2821 catcgaggga aatcgccgct ttgggctgct gcttgacccc cggcggcggc gcgcttggcg
     2881 cttgacgaac ctgcgatagc aactgcgcgc acggcagcgg tttgttattg ctaggcgcca
     2941 gcagcggaat cagtgccgcg aacgggttga tcaagccgag tccgactgcg gcgccgcctc
     3001 gcagcgccag cgcggcggca ttcacgccca cgtgcgggtc cttgaacgtc cccttcacgt
     3061 acagcggcga gcgcagcgag aacacccgga agcccttggt atgcggatgc acgccaagat
     3121 ccatggtctc gtcgcgcata ttcacattgc cgtcgatgtc gatcaccgcg tcgtccgtgt
     3181 cgagcgcgaa gacgcgcgaa tccagtacgc cgttcgtcgc aacaaagtcc gccgcggcgc
     3241 agttgatctt cacgtcgcgt ttgccgaaca gcttttcgta gacgacgttg gccacgttca
     3301 gccccgccgc ttccatcagc aggcggctca ccgtgccgtc ggtgaccaat gccttcacct
     3361 cgccgttcga cgtcgccgcc aatgccgccg gcgaattgcc cgtcgcggtc agcgcggcgt
     3421 cgccgttgat ttcaccgagc gcgttctgca tcgtcttgaa gttggggaac aactgcttga
     3481 gcttcagatg ccgtgctgag gtcgcgaagc ggcccttcag cggcgtcgtg ctgccgtcca
     3541 gatgaatatt cgatgacagc gcgccgcccg ccacgccgaa cttcagcggt tcgagcgaca
     3601 gcacgccgtc ggtcatcacg acgtgggtgt acaagtccgt gatcggcagg tcggggctct
     3661 tcacgatgcg gcggccggtg aacttgacgt cggcgtcgat cgccttccag cggtcggtgc
     3721 ggaactcttc gacaggcaac gccttgttgc ccggctgcgc cgtcgcatca ccacgcttgg
     3781 ccttgctcgc gttcgagtcc gcgccgatga tcggcgccag atcggagaat tgcagcagat
     3841 gcgacaccag ttcgccctgc aacagcggac gcggttcacg tgccgtatag atcagcgagc
     3901 cattcagatc gctgccgcct acccggcctg taaaattttc atacttgaag acgttgccgc
     3961 tggacttgaa ctggccgacc agacgcccct cggtcgcata cggcggcgtg tcgggcaagg
     4021 tcacgccggt cagcgaatag aggcgcgcca ggctgttgcc ttgcagccag aggcgcaaat
     4081 cgaccgcggc gagatgcgcc ggatcggtga tcgtgcccac gaggccgacg tgcagatcgc
     4141 cggccttcac atcggcctgc accgggaacg gccggttcgc gtcctgcaac gccagcacgc
     4201 cgcccacctt gccgctgccc aacaccggcg tcttgttgta cgtacccttc acagtccaac
     4261 cgatcgcata cggcgggatc accggcttcg cgctgttcgc cgtgccggcc tcgctggcgt
     4321 tcgacgcgga agtcaccgcc gcgccgcccg cgcccgcgct tgcaccgccc tcaccacttg
     4381 aaccgctcga cccacccgca accagcgcgc ccgaaccgcc attcgccgcc gtcgcgcccg
     4441 acgcggtgat atccgtcgac gacgcgccgg acgccgccgc cgcggaagcc gccgcggctt
     4501 ccgatacggc ctgcgcatta gcctgagcgg tgagcttgtt ggcgccggcc ttgccgatcg
     4561 cctccgcaga ggcactgcgc gaggccgcct cctgctgctt cattgcctcg ccgatcggaa
     4621 tcgattggcc gagtgtatcg atggcaatct gcatctcgac cttcttctgc tgatcggaca
     4681 gcgcgatatt gcccttggca aacgcgatgt cgtgcagatc gagcttccat tcggaaggac
     4741 ccgtggaagc cggcagcttg aaggtccagt tgttgcgccc atccagcagg cgctccagat
     4801 cgaccgacgg attcacgagg ttgatcgcgg gaatcacgat gtcgtgcgcg agcagcggca
     4861 gcaccttgac ctcgaagtcg atttcatcga gcgtggcgaa gtgcggttgt tgggtccagt
     4921 cagggttgcc gatcgtgata ttggccgccg agaaacgcgg ccagggcacc cagccccgcc
     4981 agccggtttc gccgaccgga tgccgccacc ctactttcag atcgccgttg atcgcgaacg
     5041 gccggccgat cgcctgggtg accttgtcgt cgatgtaagg tcgggcccgg ttccagttga
     5101 aggtcagaat aaaaatgatc agcgcgataa tcagaacgac gatgatcgcc accagccacg
     5161 cgatgatttt gccaatccgc cttccgatcg tgctcgacac tgccattgag aagctccgcc
     5221 ttgttcttcg ttgtggatcg gggcagcagg tttcgtgcct ggaggtcgtg tccaattggt
     5281 gtcatgtcat catgtcgcgc agttcgagcg cgggttcgcg caataattcg ggcgtagctt
     5341 gccgtcaacc aaaacgtcgt tgcaacgcct cggcgcagcg tctgattcaa cgcaaaaaat
     5401 ccaccacgat ccaacttatg accgatgttc ccttcgtcct cgccgagggc atcgcccgac
     5461 gcgatgcctt gcgcggccaa acgctgctgc aaccgaccac cttcgccctc cgcgcgggcg
     5521 accgcgtcgc ggtcaccggt ccatcgggtt cgggcaagag cgtgtttctg cgcaccctcg
     5581 ccctgctcga tccgctcgac gcaggacgca tcatgtggca tggcgcgccg gtcgagcgcg
     5641 ccgccattcc gcgttaccgg cgcaacgtcg cgtatatccg gcagcggccc gcgctgctcg
     5701 acggcagcgt cgaggacaat ttgcgctacc cgttcgaatt gcggacctat cgcgacgtgc
     5761 gtttcgatcg cgcgcgcgcc gcgaagctcg ccgcccaggc cggccgtacc gatgactttc
     5821 tcgacaagcg cgcgagcgag ttgtccggcg gcgaggcgca gatcaccgcg ctgatccggg
     5881 ttctgcaact tgcgcccgaa gtgctgctgc tcgatgaacc caccgcctca ctcgacccgg
     5941 agtcgtcgca cgcgatcgaa ggcctggtgc acgcatggtt cgatgccgag ccgggccggc
     6001 atgcgtcgat ctgggtgtcg cacgatccgg cgcaagccgc gcgcatgagc gagcggcacc
     6061 tgacgatgcg tgccggggtg ctcgatgaat ccgcggcaac gcccgcgccg caggagttcg
     6121 accgatgaca ttgcaaaacc tgagtctctg ggacgtcgcg atcgccgcgt tgctgatcgt
     6181 ggtgaacggc gtggtgtcgg tggcgctcaa gctggacctc gaacgcaagc tcgcgtgggc
     6241 ggctgtacgc accgtcgtgc aattgctggc aatcgggtat gtgctcggct gggtgttccg
     6301 ttacgaccac tggttcgtgg tgctgccgtt gatgatcgtg atgacgctga tcgccggctt
     6361 cgcgggcgcg cagcgcggca gccgcacgta tgcgggtcaa cgtgcggaca gcgtgctgtc
     6421 gatctgggtc agttcgtggc tggttgccgc ggtcggactc ttcgtcgtga tccgcattca
     6481 tccctggtac gagccgcagt atgcgattcc gattctcggc atgatcctcg gcaatacgct
     6541 caccggggtg tcgctcggta tcgaacggat gaccgaggaa ctgaccgcgc ggcgtgaccg
     6601 tgtggacatg gcgctcgcac tcggcgcgac gcgctgggaa gcggcccagg cgccggcgcg
     6661 ccaggcggtg cgggcgggca tgatgcccac gctgaatcag atggccgtgg tcggcgtggt
     6721 gagtttgccc ggcatgatga ccgggcaggt gctggccggt cagtcgccat tgcaggcggt
     6781 gcgctatcag atcgtgatca tgttcctgat cgccgcggcg tcagccttgg ggacggtggg
     6841 tgcggtgctg ctcacgtatc ggcggctctt ctcggcggag caccggtttc tggcggcacg
     6901 gctggtggag cggaccgcgg cgcggcggtg aacagcggtg agcggcggcg agtggccgta
     6961 agccgcggtg agcggcggtg agcggcgatg cgtggcggcg agcggcggtg agccgcgccg
     7021 agttgcgttc agcgagacaa caaacaggca cagggcgacg aggttgcgtc gccggctcgc
     7081 cgcccagccc actgctgcga aagcggccga tgcgttacgt acgcctcaac gtttcgcggt
     7141 caccgccact tcggtgccgt cattgagcgt cagcgtcttc gtggtgccgt tggtcggcat
     7201 cgtaaaacgc agaatctggc tgacgctcac gtcgttcgga cacttgatcg ttttgccgcc
     7261 gctcgtcacc gtctttacgc catgcggcgt ctgcgcctgg aagctcaatt gcacggacgc
     7321 ggtgccgtca ccgccgacca ccggcgcaaa gcgaatctgc gtctggcgaa tcattgcgcc
     7381 gttggtatcg accggcagcg acgcgtaatt cgggcagcta tccgtggccg gcacggggcc
     7441 gccgggcggc acggttttcc acgtgaaatc gtccgattcg ccggagcgga tcgtgcgcgt
     7501 ttcctgtgaa ttgccgaact gtttcgacgt gacgcggacc gtgtaacgaa ttggaccgtc
     7561 gagggcggat tgcgacgtca ccgtgatcgg tgtggctgcg tgagccgccg gcgcaaacat
     7621 gccgggcgcg agcgcgcacg cgagagaagc gacaactgaa acggcggaga gtttgaagct
     7681 ggagctcata ctgtcacctc gttgttgtgc cgctgcgcgc ccgcttaagc gctattgcca
     7741 ctgcaccgcc tgaccgaagc ctgatgaggc tgatgatgcc cgttgggacc cggccagacg
     7801 agccgcgaga cacgcaactg tttgagcatg atgccccacc agtctaccgc caagcggcgc
     7861 cgcgcgcgct tcatacgaat cgggtgttaa cccccttgcg ttgaagcgcc ttctgcgcgt
     7921 gcgatgtgac agaagcgccg agctcgcgcc gttcagaagc ccgtcagcac cagcttgccg
     7981 atcgcgcgcc cctcttcgag caactgatgc gcacggcgca ggttcgccgc gttgatcttg
     8041 ccgagattct ggccgaccgt cgtgcacaag gtgcccgcgt cgacgagacg cgccacctcg
     8101 gtcagcaact tatgttgctc gatcatgtcg ggggtgccga acatcgaacg cgtgaacatg
     8161 aactcccagt ggaacgcggc gcttttcgcc ttcaacagtt cgaccggcac cggctgtctg
     8221 ttttcgacga tcgagcaaat gcggccctgc ggcttgatca cttcggcggc cgccgggaaa
     8281 ttcttgtcgg tgtcgttgaa gatcagcacg taatcgacct ggtcgatgcc gagctttttc
     8341 agttgcgcgg gcatgtcgtc gaagtgatcg acgatatgat ccgcgcccaa ctccgtcgcc
     8401 catttggccg actcgggacg cgaagcggtc gcgatcactt tcagcttcgc gagttgcttg
     8461 gccaactgga tgccgatcga accgaccccg cccgccccgc cgacgatcag caccgtgcgg
     8521 ccttcatccg cgccttgcgg cgatacgccg aggcggtcga agagcgcctc ccaggcggtg
     8581 atcgcggtca acggcaacgc ggcggcgtgc gtgaaatcga acgacgccgg tttgcgcccg
     8641 acaatgcgct cgtcgaccag atgaaactcg ctattcgccc ccggccgcgt gatgctgccg
     8701 gcgtagaaaa ccggatcgcc gaccttgaac agcgtgacgt ccggtccgac cgccaccacc
     8761 gtgccggcgg cgtcccagcc gagcacacgc ggcgccttct cgacggtgtc tttcggcgcg
     8821 cgcaccttgg tgtcgaccgg attgacggaa atggcttcga ccttcaccag cagatcgcgg
     8881 ccggtggctt cgggtttggg aatctcgacg tcgaccagcg attcagcttg atcgatgggc
     8941 aaataacgat agaggcctac ggctttcatc acagctcctg ttcttttaga tgaggttggg
     9001 agatcggcga cgttaccggc ggcgtggggc gccccgcggg caatcgatcg cattgccgtc
     9061 atcctaaggg ataacggctg aaaggtcttt ttggatttca gaagaacgga tcttcctgct
     9121 cgttcgacac ccactgcaaa ccccgcggcc aaagcctcat gcagcgaacg ggaacggctc
     9181 gatccggcaa attgatgaac gtgctcgatc gttatccgct cgcggagaat gtggcgatcg
     9241 gcgcgccctg acggttcccc gcgtcgactc accggcaccc tcagcgtttg ccgcgcccgc
     9301 caccgcctgc tttgccatcg gaattgacat ccgtgtgcgc gtcattctgc gtctcttgct
     9361 gatactcgtc gccgccgcgg cggtccgtat ccttgagtcc gcgctcgacg tcgctatgtg
     9421 cctgcttgcc gacatggcgc ggattgtcct cgtgctgcga ttccgggtcc tgatcggttt
     9481 cgtgcggcag cggcgcggac gcctcctgca tcgatcgcgg atgcttcttg gcatgcgggt
     9541 cggcttgctt gccgccgtcg gacgattgcg gtgaggcctt catggcttgc tcctgtttca
     9601 gattgaatga cttcgcccgg atcgcgctca ctgcgcatcg tccagacgct ttttcattgc
     9661 cgtggtgatg cgctgacgcg tcttcgcgta gtccgcccac gggtcgcgtt taaggtcttc
     9721 gagacgctcg tgcacgtttt cgatggtcca ttgatcgccg ctcgtcgtcg tggcgacttc
     9781 gtcccatgcg agcggcactg aaacgcccag acccggccgg gcgcgcgccg aaaatgccgc
     9841 caccgtactc gagccccggt tgttgcgcag atagtcgacg aagatcttct gcttgcgatt
     9901 ctgcgcaccc atcttcgcgc tgaagtactt gggcagcgtc gccgccatat gctgcgcgac
     9961 cgcttgcgag aatgccttca cttcgtccca gccggcttgc ttcgcgagcg gcaccacgac
    10021 gtgaaacccc ttgccgccgc tcgtcttgca aaacgaagtc aggccgagtt cctcgagcag
    10081 cgagtgcgtc aattgcgcgg cctcgatcat ccgctcccaa ccgagcgacg catcggggtc
    10141 cagatcgaac accacgcgat cgggcttttc gatgttcgac gccacggcat tccatgtgtg
    10201 gaattcgatc gtgcccatct gcgcggcgcc gaccagcgcc ttgagcgtgt cgacggtaat
    10261 cagcggcgga tggccggggt cgagtccagg atgctgcgtg acgtttggaa tcgacagttt
    10321 ctggctgtgc ttctgaaaaa acagctcacc gccgaggtcc tcgggtgcgc gcaccagcga
    10381 caccggccgc tcctgcagat gcggcagcat ccagtcggca accgactcgt agtactgcac
    10441 gagatcgatt ttgcgggtgc cggtgctttt gtcgatgatg cgatcgggat gggagacgcg
    10501 aacgccggcg acttcggccg gtgtcgcggc cgtttttgat ttcgccgcgg tggctttggt
    10561 tgcgggcgtc ctggttgctg cagctttcgc tgccggcgtg cttttggccg acgtctttgc
    10621 tgcagagatt ttcgtcgcgg ccgctttcgt cgtagacggc ttcgcggcgc ttgttctggc
    10681 aggttcggct gcggcactcg cagcggtcgt tttcgccgtg cgctttttcg gcacggcctc
    10741 ggatgcggta tcggtttgct cttgcacatc ggctcctttg cggggtgctt ccttgacgat
    10801 ctgccgcgcc ggcttgtcgt cgcgcagact cacgaacgat gcctgccgca cgatgccatc
    10861 gctcgtccat tcagcaaaat tgcattcgca gacgagtacg ggctcgaccc aatgcaccgg
    10921 cgtcctgctg cgctcgcgcg gcgcgctggc gaacggcatg cgcttggtct cgtgcgca
//
LOCUS       DATASH010000779         2838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2502352,
            whole genome shotgun sequence.
ACCESSION   DATASH010000779 DATASH010000000
VERSION     DATASH010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2502352"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1229)
                     /gene="xseA"
                     /locus_tag="VHV99_20890"
     CDS             complement(<1..1229)
                     /gene="xseA"
                     /locus_tag="VHV99_20890"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012329091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEX3637893.1"
                     /translation="MNPESPFASSATPGGEVVVPVSALNRAIGTMLERSFPLVWVAGE
                     VSNFTRAASGHWYFSIKDAQAQMRCVMFRGRAQYAEFTPREGDRIEVRALVTMYEPRG
                     ELQLNVEAVRRTGQGRLYEAFLRLKAQLEAEGLFAAERKRPLPAHPRAIGIVTSLQAA
                     ALRDVLTTLSRRASHIPVIVYPATVQGAGVSTKLAAMVDAANARREVDVLIVCRGGGS
                     IEDLWAFNEEVLARAIAESAIPVVSGVGHETDFTIADFAADVRAPTPTGAAELVSPQR
                     VLLLRELDHRHATLARGFGRMMERRAQQLDWLARRLVSPAERLTRQRTHLQQWSVRLA
                     SAGARPVRDARARFSLLQMRWQRWRPDLAAHQTKLGNLAQRLDAALLRQHERQVARID
                     TLAARLEVLSPQRTLERG"
     gene            1822..2838
                     /gene="lpxK"
                     /locus_tag="VHV99_20895"
     CDS             1822..2838
                     /gene="lpxK"
                     /locus_tag="VHV99_20895"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEX3637894.1"
                     /translation="MSELNNRLEARLAREWQQRGPLAWALMPFACVFGAIAAARRAAF
                     SFGWLKSVRIGVPVVVVGNVTVGGTGKTPTVIALVEALRAAGFKPGVVSRGYGARVKG
                     PTAVFSTSAAGVGGDEPLLIARRTGAPVWVCPDRVAAAQALCAAHRDVDVIVSDDGLQ
                     HYRLARDAELVVFDHRLGGNGFLLPAGPLREPLSRRRDATLINDPYARTLPAWPNTFA
                     LKLAPADAWHLDNPGLRRPLAQFSGDRVLAAAGIGAPERFFATLRAAGLKPATRALPD
                     HYAFERNPFTDVDADAILITEKDAVKLGSWHDARIWVVPVEAALDHRLIALVVEKVRG
                     RSPA"
BASE COUNT          445 a          969 c          927 g          497 t
ORIGIN      
        1 ccgcgctcca gcgtacgctg cggactcagc acttcgagcc gcgcggccag cgtgtcgatg
       61 cgtgcaacct gacgttcatg ctgacgcaac aatgccgcgt ccagacgttg cgcgagattg
      121 ccgagcttcg tctggtgggc ggcgagatcc ggacgccagc gttgccagcg catctgcagc
      181 agcgaaaacc gcgctcgcgc atcacgcacc ggccgcgcgc cggccgacgc gagccgtacg
      241 ctccattgct gcagatgcgt gcgttgccgc gtgaggcgct cagccggcga caccagccgg
      301 cgcgcgagcc agtcgagttg ttgggcgcgc cgctccatca tgcggccgaa gccgcgggcc
      361 agcgtcgcat gccggtgatc gagctcgcgc agcaacagca cgcgctgcgg actgacaagt
      421 tcggcggcgc cggtgggcgt cggtgcgcgc acgtcggcgg caaagtcggc gatcgtgaaa
      481 tcggtttcgt gacccacgcc gcttaccacc ggtatcgcgc tttcagcgat cgcgcgcgcg
      541 agcacttctt cgttgaacgc ccacaagtct tcaatcgagc cgccgccgcg gcacacgatc
      601 agcacatcga cttcacgccg cgcgttggcc gcatcgacca tcgcggccag tttggtgctg
      661 acgccggcgc cctgcaccgt cgccgggtac acgatcaccg gaatatgcga cgcgcgacgc
      721 gacaaggtgg tcaggacgtc acgcaatgcc gcggcctgta gcgatgtcac gatgccgatc
      781 gcgcgcggat gggccggcaa cggccgtttg cgttcggcgg caaagagacc ttcggcttcg
      841 agttgcgcct tcagcctgag aaacgcttcg tacagacgcc cttgcccggt gcggcgcacc
      901 gcttcaacgt tcagttgcag ttcgccgcgc ggctcgtaca tcgtcaccag cgcgcgcact
      961 tcgatgcgat cgccttcgcg cggcgtgaac tcggcgtact gcgcgcgacc gcggaacatc
     1021 acgcagcgca tctgcgcctg cgcgtccttg atcgagaaat accagtggcc gctcgcggcg
     1081 cgcgtgaagt tcgacacctc tccggccacc cagacaagcg gaaacgagcg ctcgagcatc
     1141 gtgccaatgg cacggttgag cgcggaaacg gggacgacga cttcaccgcc gggcgtggcc
     1201 gacgaggcaa aagggctttc gggattcatg cgaggggtat ctgtgactgg ccggacagac
     1261 gatagaccga gtggccatcg gcgtctagcg cagcagaaga attgttaaaa gtgtccacat
     1321 gtcgggaccc attgcacgcg tgccgtcaga tcgcccgcca agccgcaata cttcgaagca
     1381 tgtctttgat ttatatggtg tttatatctc actaatagga acgattgccg atacggagcg
     1441 ctcggcgcgg ccttctcagc ccatcgactg gtgggttctc cacaaagtta tccacaggct
     1501 gtgaatgccg ttcgacgcgc cgtgtcgccg cgccgcgcga ggcgcccgac ttgccgggcg
     1561 gcccgcacgc gcgctagagt ggcgggttcg acggcatgcc gaacgcccca caacgctcgc
     1621 ccttgcggca cactgcacca cactgcgcga gcgcatcgcc agttgccgct gcttcacctc
     1681 tttgctgcac ttttcacttc acctcttgat tgtccggaga accgcgttga tgcttgcccc
     1741 gcgtcttgcg cctagcgctt ccacgccgcc gtcccggctc cattgggcgt gcggtgcatc
     1801 gcccgtcctt gcggcttgcc gatgagcgaa ctgaacaacc gtctcgaagc acgcctggcg
     1861 cgcgaatggc agcagcgtgg cccgctggcg tgggcgctga tgcccttcgc gtgcgtgttc
     1921 ggcgcgattg ccgccgcgcg ccgtgccgct ttctcgttcg gctggctgaa gtccgtgcgc
     1981 attggcgtgc cggtggtggt ggtcggcaat gtgaccgtcg gcggcaccgg caagacgccg
     2041 accgtcatcg cgctggtcga agccttgcgc gccgccggct tcaaacccgg cgtggtgtca
     2101 cgaggttacg gcgcgcgcgt caagggaccg acggcggttt tctccacgtc ggcagcgggc
     2161 gtcggcggcg acgagccgtt gctgatcgcg cgccgcaccg gcgcgccggt gtgggtctgc
     2221 ccggatcgcg tcgcggcggc gcaggcgcta tgcgccgcgc accgcgacgt cgacgtgatc
     2281 gtcagcgacg acggcttgca gcattaccgc ctcgcccgcg acgccgaact ggtggtgttc
     2341 gatcaccggc tcggcggcaa cggttttctg ctgccggcgg gtccgctgcg cgagccgttg
     2401 tcgcgccgcc gcgatgcgac gctgatcaac gatccgtacg ctcgcacgct gcccgcctgg
     2461 ccgaacacgt tcgcactgaa actggcgccc gccgacgcgt ggcatctcga caatccgggc
     2521 ctgcgccgcc cgctcgccca gttcagcggc gatcgcgtgc tggccgcagc cggcattggc
     2581 gcgccggagc gttttttcgc gacgctgcgc gcggccggcc tcaagcccgc cacccgcgcg
     2641 ttgccggatc actacgcatt cgagcgcaat ccttttacgg acgtcgacgc ggacgccatc
     2701 ctgataacgg aaaaggatgc ggtaaaattg gggtcctggc acgacgcgcg catctgggtt
     2761 gtcccggtcg aagccgcgct cgatcatcgc ctcattgcat tagttgtgga gaaagtccgt
     2821 ggacgctcgc ctgcttga
//
LOCUS       DATASH010000780         4009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2107269,
            whole genome shotgun sequence.
ACCESSION   DATASH010000780 DATASH010000000
VERSION     DATASH010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2107269"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VHV99_20900"
     CDS             complement(<1..464)
                     /locus_tag="VHV99_20900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HEX3637895.1"
                     /translation="MMQFTQRARAIALRTAKHAALAGVLLAGAVTAMAQTDSPVGTWQ
                     TIDDHTGQPKALVQIAQDDNGSLSGKVIKGLNANNKPDRRCTECTDARKDQLILGMTI
                     INDMQRDGDGWDHGQILDPENGKLYKCKMHLEDGGNKLVVRGYIGVSLLGRS"
     gene            complement(725..1282)
                     /locus_tag="VHV99_20905"
                     /pseudo
     CDS             complement(725..1282)
                     /locus_tag="VHV99_20905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010092524.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
     gene            1285..2159
                     /locus_tag="VHV99_20910"
                     /pseudo
     CDS             1285..2159
                     /locus_tag="VHV99_20910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006026562.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
     gene            2207..2923
                     /locus_tag="VHV99_20915"
     CDS             2207..2923
                     /locus_tag="VHV99_20915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2968 domain-containing protein"
                     /protein_id="HEX3637896.1"
                     /translation="MDRKSKLRQIALAAIVAMGAVQGVNAQTLPDNGSSAGVVSQPGT
                     TTALPANTVTGQAQTSALTPDEAKQSAAGNVAELQQMIHGSDLSELRTTYNGSYGASL
                     LFYGKEMTYYVALFQQKNFWRVIKTQDAARADMIYKDFVRQTLQLSDVEIRRTQLEAQ
                     KAFTDRMIALSQDRANRLQADLDVAHQQQTIVANQQQQTRAEATALAQQKAAAQDQLR
                     AAQRQVRELQRQLETGLPLH"
     gene            complement(3530..>4009)
                     /locus_tag="VHV99_20920"
     CDS             complement(3530..>4009)
                     /locus_tag="VHV99_20920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5 family C-terminal domain-containing
                     protein"
                     /protein_id="HEX3637897.1"
                     /translation="ESTIVDLSRGFPALLRPGRVSPQDIADVLGEAPRLPDGSDATAP
                     RASGTLKAHYAPRTPLALLPFVALEPLLSARQADERVALVARASRAGHWADAEGVHFI
                     AAPEDPHVYARELYGLLRALDRANVTRILIEKLPDTIEWIAVNDRLGRAAAAFEAQG"
BASE COUNT          843 a         1216 c         1234 g          716 t
ORIGIN      
        1 tgcgagcggc ccagcagcga aacgccaata tacccacgca ccaccagctt gtttccacca
       61 tcttccaggt gcatcttgca cttgtagagc ttgccgtttt ccgggtcgag aatctgcccg
      121 tgatcccagc cgtcgccgtc cctttgcata tcgttgatga tcgtcatgcc gagaatcaac
      181 tggtccttgc gcgcgtcggt acattccgtg cagcggcggt ccggtttgtt gtttgcattg
      241 agacccttga tcaccttgcc gctcagcgag ccattgtcgt cctgtgcaat ctgcacgagt
      301 gctttgggct ggccggtgtg gtcgtcaatg gtctgccacg tgccgaccgg cgaatcggtc
      361 tgcgccattg ccgtgacggc gccggcgagc agcacgccgg cgagggcggc atgtttagcg
      421 gtacgaagcg cgatagcgcg cgcgcgttga gtgaattgca tcattgtctt cctccttgat
      481 taaagacggt gcgatcatgg tcgcgcggcg ttatcggcat ttcgggcggt cttcctggtg
      541 gccgccgtgg tgcctggtct gcccgatatg tcttattgct gcctctcagg ttcaacttcg
      601 gctacatcgg tccgcgagcc gcggcttgcg tgccgcggct cgcacatcgt cagcgcagaa
      661 tacgtgaaag taggcacgcc gtttgatagt ggaacctcta atcaggacgt ggcacttttg
      721 aatctcagtt cagctgatat gagaaagtag cgttgatgcg cgggctgcgc gacgcgcttt
      781 cgaccggcgc gtcgccgatc ggtttggcca ccgacaaatc caggctgtag tacctcgcgt
      841 ccgagatccg gaagccgaag gcaatcgacg acagcttcga cggcgacggc gtccccgcat
      901 gcagatacac gcgcgccatg tcgaacgaga tatacggtgt gagggtacgc agataggtga
      961 aacccggcgt gaacgcccgg ttgatttcga gcatcgcgcc ccatcccgaa tcgcccgacg
     1021 cctcgcccgg ttgatagcct tgtgcaaaac gttgcgcaac gaacgagatc tgttccgagg
     1081 tcggcagcga gacggcgctg tattgacccg tcaacgacac cgcggtgccg atcttcagcg
     1141 gccactggtt ggtgtgcgag aagctcgcgc cggttcgcac gaaagtgatc gacgccgggt
     1201 tcgtgacgat ggtgccaagg acgttcgagt cgccggactt cgacgcgccc aggatgtcga
     1261 acgccttcgc cacgttgata ctcgcgcatc gcgcaactgc tgcacgaaaa acatactcgc
     1321 tacggccagg ggccgttcgt cgcggtgaac tgcggtgcga ttccggacgg tctgttcgaa
     1381 tccctgctct tcggccactc gaaaggctcg ttcaccggcg cggtggtggc gcacaaaggt
     1441 tacttcgagc aggccgacgg cggcacgctg ttcctcgacg aaattggcga tctgccgctc
     1501 taccagcagg tcaagctgct gcgcgtgccg gaagacagcg cggtcacgcg aatcggctcg
     1561 gcgacgcccg tgaagctgga tttccggctg gtggccgcga ccaacaagca cctgccgcaa
     1621 ttggtgaaag agggcgcctt ccgcgcggat ttttactatc ggcttgcggt gatcgagttg
     1681 aagattccgt cactggaaga gcggggcgct gtcgacaaga tcgcgatctt caaggcattc
     1741 atcgcgcaga tcgtcggcgg cgagcggctt gcggctttgc cggacctgcc gtactggctc
     1801 gccgacgcgg tggccgacac ctacttcccc ggcaatgtgc gcgaactgcg caacctggcc
     1861 gagcggatcg gcgtgacggt acggcagatc ggcgcgtggg acgcgtgcgc ctgcagcggc
     1921 tgctcgcgct ggcgcgcgcc agccagccgg tgccggccga aagcgcggcc gaggtgccgg
     1981 tggatcgcag caaatgggac atggccgaac gcaaccgcgt gctggccgcg ctcgacgcaa
     2041 acggttggcg acgccaggat acggcactct atctgggcat cagccggaag gttttgtggg
     2101 agaagatgcg caagtaccaa atttttgacg aagagcccga aacacgcgaa agtgagtaat
     2161 aatgagatgg ttgtactaaa acaaaatctg ttcaagcagg atttacatgg accgaaagtc
     2221 gaaactacgg cagattgccc tggctgcaat cgtcgcgatg ggggccgtgc agggcgtgaa
     2281 tgcgcagact ctgccggata acgggtcgag cgcgggcgtc gtttcccagc cgggcacgac
     2341 gacggctttg ccggccaata cagtgacagg acaggcgcag acgagcgcat tgacaccgga
     2401 cgaagcgaag cagtctgccg cgggcaatgt tgcggaattg cagcagatga ttcacggctc
     2461 ggatctgagc gaattgcgca cgacgtacaa cggcagctac ggtgcgagct tgctgtttta
     2521 cggcaaggag atgacgtact acgtggcgtt gttccagcag aagaacttct ggcgcgtcat
     2581 caagacgcag gacgctgcgc gtgccgacat gatctacaag gattttgtgc gtcagacgct
     2641 gcaactgtcg gacgtcgaga tacgccgcac gcaattggaa gcgcagaagg cgttcacgga
     2701 tcggatgatt gcattgtcgc aggaccgggc caaccgcttg caggccgacc tggacgttgc
     2761 gcaccagcag cagaccattg ttgcgaatca gcagcaacag acccgtgccg aggccacggc
     2821 gctggcgcag cagaaggctg ccgcccagga ccagctgcgc gccgcgcaac gccaggtccg
     2881 cgaactgcag cgccaactgg aaacgggcct gccgttgcat tgagtgcgat ggtcggaaga
     2941 tggatgggcg gagcggcgtt aaaagtgccg cccgacccac tgaccgtacg agcggttgta
     3001 gtaatgcaag tcacttctcc cggcgacaca aagcaaagtc gcactgcgta aagccacgca
     3061 gtacaggacc tcgcggcaca aaacccagcg gtacaagcct gtggcacaaa gcccagcgac
     3121 acaaagccca gcgacacaaa gcctgcggta caaaggcccg caatccaaag cctgtgggaa
     3181 gcaacgttgc tgcctgaggg ggcgcccttt gtgcccactg gtttccttgg catacccatc
     3241 cgcgacgcac gtagtgcgga gtgggagcag atgagcgcct tgtcactagc gctgaatgtg
     3301 cgagaacacg gattccagat gcaccactac tgcgactgtg cgggggaccc gcttaggcgc
     3361 tggcggtgtg agccgctttc ttttgcctac ttttctttgc ggccggcaaa gaaaagtagg
     3421 tgccgccccg cacaggggca acactaataa accactaaca aatcaaggaa aggccaacac
     3481 cgccgccaac cagaaaaaag caccgcccag gcaaaaaaaa cccaacccat cacccttgcg
     3541 cttcaaaagc agccgcagcc cgccctaacc ggtcattaac agcaatccac tcaatcgtat
     3601 ccggcagctt ctcgatcaga atccgcgtga cattcgcgcg atcaagagcc ctcagcaaac
     3661 catacaattc acgcgcatag acatgcggat cctcaggcgc agcgatgaaa tgcacacctt
     3721 cggcatcggc ccaatgtccg gcacgcgacg cgcgcgccac caacgccacg cgctcgtccg
     3781 cttgccgtgc ggaaagcaaa ggctccagcg cgacgaaggg caatagcgcc aaaggcgtac
     3841 gcggcgcgta gtgcgccttc aacgtgccgg aagcccgcgg cgcggtcgca tccgagccgt
     3901 ccggcaaacg cggcgcctcg ccgagcacgt cggcgatatc ttgcggactc acacgccccg
     3961 gcctcaaaag cgccggaaaa ccgcgcgaca aatccacaat ggtcgattc
//
LOCUS       DATASH010000781         6841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5470956,
            whole genome shotgun sequence.
ACCESSION   DATASH010000781 DATASH010000000
VERSION     DATASH010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6841
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5470956"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VHV99_20925"
     CDS             complement(<1..325)
                     /locus_tag="VHV99_20925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006025722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX3637898.1"
                     /translation="MFRKILVTAAMLAAFGHAYAAVDVNTANEDALRGIKGIGPAKAK
                     AILEERSAHGPFKDPADLGKRVKGMGGHTVERLQAEGLAVGPAGAAANTQAAASAQSK
                     GAAAAG"
     gene            complement(403..1395)
                     /gene="rfaD"
                     /locus_tag="VHV99_20930"
     CDS             complement(403..1395)
                     /gene="rfaD"
                     /locus_tag="VHV99_20930"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HEX3637899.1"
                     /translation="MTLIVTGAAGFIGSNLVKALNERGEQRIIAVDNLTRADKFKNLV
                     DCEIDDYLDKTEFVERFKRGDFGKVRAIFHEGACSDTMETDGRYMMDNNFRFSRDVLD
                     VCLAQNIQFLYASSAATYGGSSRFVEEREVEQPLNVYGYSKFLFDQVIRRVLPAAKSQ
                     IAGFRYFNVYGPRETHKARMASVAFHNFNQFRAEGKVKLFGEYNGYAAGEQTRDFVSV
                     EDVVKVNLFFFDNPDKSGIFNLGSGRAQPFNDIASTVVNTLRALNNEPPLSLADQVQR
                     GLIEYIPFPDALRGKYQCFTQADLTKLRAAGYDAPFLSVQEGVDRYVRWLFGQV"
     gene            complement(1501..2508)
                     /gene="rfaE1"
                     /locus_tag="VHV99_20935"
     CDS             complement(1501..2508)
                     /gene="rfaE1"
                     /locus_tag="VHV99_20935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HEX3637900.1"
                     /translation="MPSPLNFRPVSPTMPEAVQSMPAAALIVVPRERLGAARVLVVGD
                     VMLDRYWFGDVNRISPEAPVPVVHVQRQEDRLGGAANVARNAVALGAQAGLLCVVGHD
                     EPGERIVQLLGESGVAPHLERDPDLLTTIKLRVLSRQQQLLRVDFENSPAHEVLLAGL
                     ARFDDLLPSHDVILMSDYAKGGLTHVTQMIAKAHAAGKPVLVDPKGDDWERYRGATLI
                     TPNRAELREVVGQWKSEEDLIARVTRLRADLEFKALLLTRSEEGMTLFSDDGILHASA
                     VAREVYDVSGAGDTVIATLAAMLGAGLTLVEAVGLANRAAGIVVGKLGTATVTYDELF
                     H"
     gene            complement(2462..3877)
                     /locus_tag="VHV99_20940"
     CDS             complement(2462..3877)
                     /locus_tag="VHV99_20940"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEX3637901.1"
                     /translation="MKITIIGTGYVGLVTGACLAEIGHDVFCLDVDPRKIEILNNGGV
                     PIHEPGLLEIIARTRKARRITFSTDIEASVAHGEVQFIAVGTPPDEDGSADLQYVLEA
                     ARNIGRTMNGFKVIVDKSTVPVGTAQRVRAVVEEELAKRGLAHSEQHRFSVVSNPEFL
                     KEGAAVDDFMRPDRIVLGSDEDEAGLRARELMKRLYAPFNRNHERTMYMDVRSAEFTK
                     YAANAMLATRISFMNEMSNLADRVGADIEAVRRGIGSDPRIGYHFLYAGCGYGGSCFP
                     KDVQALIRTASESGHNLRILEAVEEVNYKQKDVLVQKITATLGEDLSGRTFAVWGLAF
                     KPNTDDMREAPSRRVIAELLARGAQVRAYDPVAVAEARRVFAIDLHDAPEQQARLTFT
                     STQDETLTGADALVVVTEWKEFKSPDFVHLKSVLKSPLIFDGRNLYEPDAMIELGIDY
                     HSIGRPYAQPTEFPAGVANHA"
     gene            complement(4004..5179)
                     /gene="lapB"
                     /locus_tag="VHV99_20945"
     CDS             complement(4004..5179)
                     /gene="lapB"
                     /locus_tag="VHV99_20945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HEX3637902.1"
                     /translation="MDLDFWWLLVIPVAFAFGWMAARYDLKTLLSESANLPRSYFRGL
                     NFLLNEQPDQAIDAFIEVVKLDPETIELHFALGNLFRRRGETDRAIRVHQNLLSRTDL
                     PVTERDHALYELGQDFLKAGLLDRAEETFRSLETGNYALGAQRALLTIYQIEKDWVKS
                     IDIARHLETMGAESLDKEIAQFHCELAQEALQQKNADEARRQLGFALKANPTNVRATI
                     LFGDVDATAGLQEAAIVQWRRVEEQNPAYLPLVAEKLMKAYEALGRPQDGADLLTTWV
                     DRYPSNDLLDVTYQHIAAIRGPDAAHALARSQMQKSPNLAGMTRLLEAQEAAAEEPRR
                     SELELMRTLIRQRTKNLPRYTCQNCGFRARLFYWQCPGCSGWETYAPRRVEPITASN"
     gene            complement(5249..5542)
                     /locus_tag="VHV99_20950"
     CDS             complement(5249..5542)
                     /locus_tag="VHV99_20950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HEX3637903.1"
                     /translation="MKFIVWLIRVLVFVLLLVLALSNTQPATLNFLAGYAWSAPLILI
                     GLAFFVVGLLAGLVSSVPAMVRLRMENGRLKRELRVAREAPVVVEQPPMPPLI"
     gene            complement(5936..6262)
                     /locus_tag="VHV99_20955"
     CDS             complement(5936..6262)
                     /locus_tag="VHV99_20955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006998681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEX3637904.1"
                     /translation="MTKSELVAQLATRFPQLVLKDADFAVKTMLDAMSEALANGHRIE
                     IRGFGSFGLNRRPSRVGRNPKSGEKVLVPEKHVPHFKPGKELRERVDRRSGEPLKADE
                     PDDDDL"
     gene            complement(6295..>6841)
                     /locus_tag="VHV99_20960"
     CDS             complement(6295..>6841)
                     /locus_tag="VHV99_20960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX3637905.1"
                     /translation="GLPGGIDGLVHLSDLSWSETGEEAVRKYKKGDEVEAIVLGIDVE
                     KERISLGIKQLEGDPFSNFVAMNDKGSIVDGVVKTVDAKGAVVQLSAEVEGYLRASEI
                     AQDRVEDARNVLKEGDKVNAMIINIDRKSRGINLSIKAKDSAEQQEAIRGLASSDSSS
                     AATGTTNLGALLKAKLDGQNQ"
BASE COUNT         1302 a         2178 c         2051 g         1310 t
ORIGIN      
        1 tgcccgcagc ggccgcgccc ttggactgtg cagacgccgc agcctgcgtg ttagcagccg
       61 cgccagccgg accgacagcg agaccctccg cctgcaggcg ctcgacggta tgcccgccca
      121 tgcctttgac gcgcttgccg agatcggccg ggtccttgaa cggaccatgc gctgaacgct
      181 cttcgagaat ggctttcgct ttggcaggac cgatgccctt gatgccgcgc agcgcgtcct
      241 cgttagccgt gttgacgtcg accgccgcat acgcgtggcc aaaagcggcg agcatggccg
      301 cggtaaccag aatttttcga aacatatgga atctccctcg tacaaccggt tggcgaccat
      361 caccaaccgg gagattccag tttaaagagg tatccgaaag atttacacct ggccgaatag
      421 ccaacgtacg tatcgatcga caccttcctg cacgctcagg aacggcgcgt cgtaaccggc
      481 ggcgcgcagc ttcgtcaggt cagcctgcgt gaagcattga tacttgccgc gcaatgcatc
      541 ggggaacgga atgtattcga tcagcccgcg ttgcacctga tccgccagcg acaagggcgg
      601 ctcgttgttc aacgcacgca gcgtgttgac caccgtgctc gcgatgtcgt taaacggctg
      661 cgcacggcca ctgcccaggt tgaaaatgcc cgacttgtcc ggattatcga agaagaacag
      721 gttgaccttg accacgtctt cgacggagac gaagtcgcgc gtctgttcgc cggcagcata
      781 accgttgtac tcgccgaaca gcttgacctt gccctcggcg cggaattgat tgaagttatg
      841 aaacgccacc gacgccatgc gcgccttgtg cgtctcgcgc ggtccgtaca cgttgaaata
      901 gcggaagccg gcaatctgac tcttcgcggc gggcagcacg cggcgaatca cctggtcgaa
      961 caggaacttc gaatagccgt acacgttcag cggctgctcg acttcgcgct cttcgacgaa
     1021 gcggctcgag ccgccatacg ttgccgccga ggacgcatac agaaactgga tattctgcgc
     1081 gagacagaca tccagcacgt cacggctaaa gcggaagttg ttgtccatca tgtagcggcc
     1141 gtcggtttcc atcgtgtccg agcaggcgcc ttcgtggaaa atcgcgcgca ctttgccgaa
     1201 gtcgccccgc ttgaagcgtt cgacgaactc ggtcttgtcg agataatcgt cgatctcgca
     1261 gtcgacgagg ttcttgaatt tgtccgcgcg cgtgaggttg tcgaccgcga tgatgcgttg
     1321 ttcaccgcgc tcattgagcg ccttcacgag attgctgccg ataaagccgg ccgcgccggt
     1381 gacgatgagg gtcatgatga tcctgcggta atggtgttca gttgacgcct cgctgcttcc
     1441 ggcggattcc gctgcctcgc gtcactagcg ggtgcagggc agggaacccg gcgggccgca
     1501 tcaatggaag agttcgtcgt aggtgacggt ggcggtgccg agcttgccga ccacgatgcc
     1561 ggccgcgcga ttcgcgagcc cgaccgcctc gaccagcgtc aaacccgcgc cgagcatggc
     1621 ggcgagcgtg gcgatcacgg tgtcgcccgc gcccgacaca tcatacactt cgcgggcaac
     1681 cgccgaagcg tgcaggatgc cgtcgtcgga gaagagcgtc atgccctctt ccgagcgcgt
     1741 cagcagcaac gccttgaact cgagatcggc gcgcagcctg gtgacgcgcg cgatcagatc
     1801 ttcctcggat ttccactgtc cgaccacctc gcgcaactcg gcgcgattcg gcgtaatcag
     1861 cgtggcgccg cgatagcgct cccagtcgtc gcccttcgga tcgaccagca caggcttgcc
     1921 cgccgcatgc gccttcgcga tcatctgcgt gacgtgcgtg agaccgccct tcgcgtagtc
     1981 ggacatcagg atcacgtcgt gcgacggcag caggtcgtcg aaacgcgcga ggcccgcgag
     2041 cagcacttcg tgcgccggtg agttctcgaa gtcgacgcgc agcaactgct gctgacgcga
     2101 cagcacgcgc agcttgatgg tggtgagcag atccggatca cgctcgagat gcggcgccac
     2161 accgctttcg ccgagcaact gcacgatgcg ctcaccgggc tcgtcatggc cgaccacgca
     2221 cagcaaaccc gcttgcgcgc cgagcgcaac cgcattgcgc gccacattcg ccgcaccgcc
     2281 gaggcggtct tcctggcgtt gcacatgcac gaccggcact ggcgcttccg gcgaaatacg
     2341 gttcacgtcg ccgaaccagt agcgatccag catcacgtcg ccgaccacca gcacgcgcgc
     2401 cgcgccgagg cgttcgcgcg gcaccacgat aagtgccgcc gcaggcatgg actgaacggc
     2461 ctcaggcatg gttggcgaca ccggccggaa attcagtggg ctgggcatag ggtcgtccga
     2521 tcgagtgata gtcgatgccg agctcgatca tcgcgtccgg ctcgtacaga ttgcggccgt
     2581 cgaaaatcag cggcgacttc agcaccgatt tcaagtgcac gaaatccggg ctcttgaatt
     2641 ccttccattc ggtgactacc accagcgcgt cggcaccggt gagcgtttcg tcctgggtgc
     2701 tcgtgaatgt gagacgcgct tgctgctccg gcgcatcgtg cagatcgatg gcgaacacgc
     2761 ggcgcgcttc agccaccgcc accggatcgt atgcgcggac ctgagcgccg cgcgccagca
     2821 gttcagcgat cacgcgacgg ctcggcgcct cgcgcatgtc gtcggtattc ggcttgaatg
     2881 cgagccccca cacggcgaac gtgcggccgc tcagatcttc accgagcgtg gccgtgatct
     2941 tctgcaccag cacgtctttc tgcttgtagt tcacttcctc gaccgcttcg aggatgcgca
     3001 gattgtggcc gctttcgctg gcggtacgga tcagcgcctg cacgtccttc gggaagcacg
     3061 acccgccgta accgcaaccc gcatacagaa agtgatagcc gatacgcggg tccgagccga
     3121 tgccgcgacg caccgcttcg atatccgcgc cgacccggtc cgccagattc gacatctcgt
     3181 tcatgaacga aatgcgcgtg gcgagcatcg cattggccgc gtatttcgtg aattcagccg
     3241 agcgcacgtc catgtacatc gtacgttcgt gattgcggtt gaacggcgcg tacagacgct
     3301 tcatcagctc gcgcgcgcgc aggccggctt cgtcttcgtc gctgccgagc acaatgcgat
     3361 cggggcgcat gaagtcgtcg acggccgcgc cctccttcag aaactccggg ttggatacca
     3421 ctgagaagcg gtgctgctcg ctatgggcga ggccgcgttt ggccagctcc tcttcgacca
     3481 ccgcgcgcac gcgttgcgcc gtgccgaccg gcaccgtcga tttgtcgacg atcaccttga
     3541 agccgttcat cgtacggccg atgttgcgcg ccgcttcgag cacgtattgc aggtcggccg
     3601 agccgtcttc gtcgggtggc gtgccgacgg cgatgaactg cacctcgccg tgcgccacgc
     3661 tcgcttcgat atccgtcgaa aacgtgatgc ggcgagcctt gcgcgtacgg gcgatgatct
     3721 ccagcaaacc cggctcgtgg atcggcacac cgccgttgtt gaggatttcg atcttgcgcg
     3781 gatcgacgtc gagacagaag acgtcatggc cgatttcggc gaggcacgcg ccggtgacga
     3841 ggccaacata gcccgtgccg ataatggtga ttttcatacg ctttccggtg gagggttccg
     3901 gtgagggttt ctcgtagtga acaaggccgt catgatcccg cctggcctac ttcgcctggc
     3961 gggccgccgt ctttgcggct cgactacacg attgcatgcg cgatcagttc gacgccgtga
     4021 tcggttcgac gcgacgcggc gcataggttt cccaaccgct acaacccggg cactgccaat
     4081 agaacagccg cgccctgaaa ccgcaattct ggcacgtata ccgcggcaag ttttttgtgc
     4141 gctgacggat cagcgtacgc atcagttcga gctcgctgcg ccgcggctct tccgcggctg
     4201 cttcctgagc ctccagaaga cgcgtcatgc ccgccagatt cggcgacttc tgcatctgcg
     4261 agcgcgccag cgcatgcgcg gcgtcgggcc cgcggattgc cgcaatgtgc tgataggtca
     4321 cgtcgagcag atcgttcgac ggatagcggt ccacccacgt agtgagcaga tcggcgccat
     4381 cctgcggacg gccgagcgcc tcataggcct tcatcagctt ttcggcgacg agcggcagat
     4441 aggcgggatt ctgttcctcg acgcgccgcc actggacgat cgccgcctct tgcaaacccg
     4501 ctgttgcatc aacgtcaccg aacagaatcg tggcgcgtac gttggtcggg ttcgccttca
     4561 atgcaaaacc tagctgacga cgcgcctcgt ccgcgttttt ctgctgaagc gcctcctggg
     4621 cgagctcgca atggaactgc gcaatctcct tgtcgagcga ctcggcgccc atggtttcca
     4681 ggtggcgcgc gatgtcgatc gacttaaccc agtctttctc gatctggtag atggtgagca
     4741 gcgcacgctg cgcgcccaac gcgtaattac cggtttcgag cgagcgaaaa gtctcttcgg
     4801 cgcgatcgag caaaccggcc ttcagaaaat cctggcccag ttcatacagt gcgtgatcgc
     4861 gctcggtgac gggcagatcg gtgcggctca acaggttctg atgcacgcga atcgcgcggt
     4921 cggtctcgcc gcggcggcgg aacagattcc caagcgcaaa gtgcaactcg atcgtctccg
     4981 gatcgagctt gacgacttcg ataaatgcgt caatcgcctg gtcgggctgc tcgttgagca
     5041 ggaaattcag gccgcggaag tatgagcgcg gcaggttggc gctttcggac agcagcgtct
     5101 taaggtcgta gcgcgcggcc atccagccga acgcaaacgc gacgggtatg acaagcagcc
     5161 accagaagtc tagatccatg cgattaatgc aaatgtgcgg gacagcggaa acggaaagcc
     5221 gcgcctcgga agcgcggcag aagaaagctt agatgagcgg cggcatgggc ggttgttcga
     5281 ccaccaccgg ggcttcacgc gccacacgca actcgcgctt gagccggcca ttctccatgc
     5341 gcaggcgcac catggccggc accgacgaca ccagcccggc cagcagcccc accacgaaga
     5401 acgcgaggcc gatcagaatc aagggcgccg accacgcgta gccagcgagg aaattcagcg
     5461 tagcaggttg tgtattggag agtgcgagca ccagcagcag cacgaacacc agcacacgga
     5521 tcagccagac gataaatttc atgaataggt ctcttgacgg cagttgcggg gtgacgccgg
     5581 cttgatgacg cgtcgcgcca cggtgttgcc cgcgccgaag tgacccgtat tgtaattgaa
     5641 gccctcgcgg ctgggcagga agtggctgat tgaggacttg tgcgaggttg atcgtgccgc
     5701 aggcgcatgc ccgcgcgggc atgctggatt ttgaggatac accgcgggcg tacagggtcc
     5761 gccacgtcaa gcctgccagc gcaaaagcac tgtcgacaac aacgatgagc gaaaactacc
     5821 gacgaaaact gcggacaaaa aaaagcgccc gaaggcgcct ttttctggtc ttttgccgtc
     5881 tggctttggc cgctgacgct accaatgcca cgctgccaat cgcacagcga agcatttaca
     5941 ggtcgtcgtc atccggctca tcggccttca gcggctcgcc cgaacgtcga tctacccgct
     6001 cgcgcaattc cttgcccggc ttgaagtgcg gtacgtgttt ctcaggtacc aacaccttct
     6061 cgcccgactt cggattacgc ccgacgcggg agggacgacg attaaggccg aagctgccga
     6121 agccgcgaat ctcaatgcga tgaccgttgg caagcgcctc cgacatcgca tcaagcatcg
     6181 tcttcaccgc gaaatccgca tctttaagta caagctgcgg aaatcgcgta gccagctggg
     6241 cgaccaattc cgatttggtc atactgcagc agacctctca agaaaactca aggcttactg
     6301 attctggccg tcgagcttgg ccttcagcag cgcgccgagg ttcgtcgtgc cagtggcagc
     6361 cgagctcgag tccgacgaag ccaggccgcg gatcgcttcc tgttgctcag ccgaatcctt
     6421 agccttgatc gacaggttga tgccacgcga cttgcgatcg atgttgatga tcatcgcgtt
     6481 gaccttgtcg ccttccttca gcacgttgcg agcatcttcc acgcggtctt gtgcgatttc
     6541 cgaagcgcgc aggtagcctt cgacttctgc cgacagctga accactgcac cctttgcgtc
     6601 taccgtcttg accacgccgt cgacgatcga acccttgtcg ttcattgcaa cgaagttgct
     6661 gaacgggtcg ccttcgagtt gcttgatacc cagcgaaata cgttccttct cgacgtcgat
     6721 gccgagaacg atcgcttcca cttcgtcgcc cttcttgtac ttgcgaactg cttcttcgcc
     6781 cgtttcgctc cacgacaggt ccgacaggtg aaccagaccg tcgatgccgc ccggcagacc
     6841 g
//
LOCUS       DATASH010000782         6507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_129792,
            whole genome shotgun sequence.
ACCESSION   DATASH010000782 DATASH010000000
VERSION     DATASH010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6507
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129792"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /locus_tag="VHV99_20965"
     CDS             <1..1071
                     /locus_tag="VHV99_20965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX3637906.1"
                     /translation="LNLQMDRTRLQQLNLSASNVAQNVLISLSGSSQTSPGFWFNNKN
                     GVEYNVAVQTPQYQVSSIDELLRTPVSGSATGPTQLLGNLVLVSPQTQFAEVTHYNIR
                     PVIDLYVSVEKRDLGSVANQVDKLVDNVRASLPRGSQITVRGQVQTMRSSFFGLGLGV
                     AMAIVLVYLLIVVNFQSWVDPLIIVSALPAALAGIVWMLFLTGTHLSVPALTGAIMTM
                     GVATANSILMVSFARQRLSAGAPPLTAALEAGASRIRPVLMTAFAMIIGMIPMALGLG
                     EGAEQNAPLGRAVIGGLLFATVSTLFFVPLVFAGIHTRLARRHRDDDDTDTTGNHGNH
                     GESGGHGNDGPAPDHGGAGKPA"
     gene            1139..2362
                     /locus_tag="VHV99_20970"
     CDS             1139..2362
                     /locus_tag="VHV99_20970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX3637907.1"
                     /translation="MTEKTHASLAIPARETEGGHALPPRHREWKRAKIAIWIVLALLA
                     VGALRTVIADIMQSRSVADTTKQNATQYVNVVSPTQTEDGGNTLLPGTLRGNVESPIY
                     ARSNGYLLHWYADIGARVKQGQLLADLDTPEIDQELAQALAQRQQTSSSLGLAKSSLD
                     RWQQLRQRDAVSQQELDERQSTFSQDVANLAAADANVKRLQQLESFKRIVAPFAGVVT
                     QRNVDVGDLIDAGSGTSRALFALAQSDPLRVYVQLPQAYAQNVSVGQKVVVTQAELPD
                     QQFHGTITHISGAIDVPTRSLQVEVTLPNPDGKLRPGAYVQVAVPAVAHARLTVPGNA
                     LLFRAEGPRVAVVDANGNVVLHKIVIARDLGQSLEIESGIEANDKVIINPSDSIADGD
                     HVQITQPQKNGKGAS"
     gene            2404..3975
                     /locus_tag="VHV99_20975"
     CDS             2404..3975
                     /locus_tag="VHV99_20975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEX3637908.1"
                     /translation="MAQGALASAASAALLAACTVGPDYQRPQAEVPPSWQTDSYWRVA
                     VPSHAPISPDWWSTFRDPTLGALETQALAQNQTLAAASAHYEQSRATLANTRAQQIPE
                     VDAFAAASRFRISSDRPLTNYATPTQSTVQNSLQLGPTINYDTDLFGRIRREVESAAA
                     STQQSADDLANARLVLTTDLATDYFSLRELDAEIDVLNQSVKLQQKALDYVTTEHDLG
                     VVSGLDVLQQKSLLDSTRVQAQLLLNQRAQFEHAIAALVGVPAPQFAIEPKVLDVQVP
                     SIPLGLPSDVLQRRPDIASAERAMAAANAQIGVAKAAFFPSLTLTPSIGWETTQFASL
                     LSAPTLMWTLGAAVGQVLFDGGRRAANVQFASEGYKATEATYRQTVLNAFQQVQDGIT
                     GLSVLDGASKQSHEAVTDAQHLLALANDRYSGGLAAYLDVITAQQSLLTSERQDVQIH
                     GQQMTLSVSLVKALGGGWEAGTDVSGAQPRGNTTGTANAANTANAANTVNIATTANIA
                     NTKEAMTPAKSVARQ"
     gene            4039..4725
                     /locus_tag="VHV99_20980"
     CDS             4039..4725
                     /locus_tag="VHV99_20980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal response regulator transcription
                     factor"
                     /protein_id="HEX3637909.1"
                     /translation="MKLLIVEDEHKVVDYLRSGLTEQGWVVDVALDGEEGMHLATEFD
                     YDVIVLDVMLPKRDGFSVLKALRMRKSTPVIMLTARDHVNDRVRGLREGADDYLTKPF
                     SFLELVERLHALARRTRSQESTLISVGDLFVDLIGRRATRDGVRLDLTAKEFQLLSVL
                     ARRQGDILSKTAITELVWDVNFDSHTNVVETAIKRLRAKLDGPFSSKLLHTVRGMGYV
                     LEVREEAEQT"
     gene            4722..6179
                     /locus_tag="VHV99_20985"
     CDS             4722..6179
                     /locus_tag="VHV99_20985"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HEX3637910.1"
                     /translation="MNRSIARRLASMFALVALFVFTLIGTGLFLVLRSQLEHHLRESL
                     DDRTQIARIIVYHAVTPDKWRMAREKLTDMTPHDASTVYSISSTDPYFHYGNPVDGTV
                     VKRWPGGYARVTPPGGAEDMLTSTVTIPAYANRPPVQLQVAASYSPNVRTMRVFGSAL
                     AALSALGSLAVLLLSYSVTRLGLAPLTRLTRDASAVSPNNRSQRLNTASLPLELNDLA
                     NSFNGALERLDGAYGRLESFNADVAHELRTPVTILIGQTEVALTRNRSVDNLRQTLQS
                     NLEEFERMRAIINDMLFLARADQGERATGLIELSLAAEVAHTLEFLEIPLEEARVSAQ
                     LRGDAVTRVNRSLFGRACANLLMNAIQHCEPGAEITVTITRDADQLRVAVANPGAPIE
                     PAVLEHLFVRFYRAEESRTNSRENHGLGLAIVKAIAEMHRGTVSVESANGVNTFAFSV
                     AALRAEGDSPAAPRSSAGVTAEAGGYSSLVKGKSA"
     gene            complement(6146..6460)
                     /locus_tag="VHV99_20990"
                     /pseudo
     CDS             complement(6146..6460)
                     /locus_tag="VHV99_20990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491272.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
BASE COUNT         1215 a         2070 c         2105 g         1117 t
ORIGIN      
        1 ctcaacctcc agatggaccg cacgcgtctg cagcaactca atctgagcgc gagcaacgtc
       61 gcgcagaacg tgctgatttc gttgtcgggc agctcgcaga cgtcgccggg cttctggttc
      121 aacaacaaga acggcgtcga gtacaacgtc gcggtgcaga cgccgcagta ccaggtatcg
      181 tcgatcgacg aactgttgcg cacgccggta tcgggttccg ccaccgggcc cacgcaactg
      241 ctcggcaacc tggtgctggt ctcgccgcag acccagttcg cggaagtcac gcactacaac
      301 atcaggccgg tgatcgacct gtacgtgagc gtcgagaagc gcgacctcgg cagcgtggcg
      361 aatcaggtcg acaagctcgt ggacaatgtg cgtgcgtcgc tgccgcgcgg cagtcagatc
      421 accgtgcgcg gccaggtgca gaccatgcgc agttcgttct tcgggctcgg cctgggcgtg
      481 gcgatggcaa tcgtgctggt ctatctgctg atcgtggtga atttccagtc gtgggtcgat
      541 ccgctgatca tcgtcagcgc gttgccggcg gcgctagcgg gcatcgtgtg gatgctgttt
      601 ttgaccggca cgcatctgag cgtgccggcc ctgaccggcg cgatcatgac gatgggcgtc
      661 gccaccgcca acagtatttt gatggtgtcg ttcgcgcgcc agcggctctc cgccggtgca
      721 ccgccgttga cggccgcgct cgaggccggt gccagccgga tcaggccggt gctgatgacc
      781 gcgttcgcca tgatcatcgg catgattccg atggcgctcg ggctgggcga gggcgctgag
      841 cagaatgcgc cgctcggccg cgcggtgatc ggcggcttgc tgttcgccac tgtgtccacg
      901 ctgtttttcg tgccgctggt gttcgccggc attcataccc gcctggcgcg ccgccatcgc
      961 gacgatgacg acacggacac gacgggcaac cacggcaacc atggcgagag cggcggccac
     1021 ggcaatgacg gccccgcgcc ggaccacggc ggcgcaggca agcccgcgta acgttcaatg
     1081 taaatgacgg ccggctgcac tggccgactg agatggctga ctgagatggc tgactgagat
     1141 gaccgaaaaa actcatgcat cgctggcgat tcccgcgcga gagactgaag gtggccatgc
     1201 cttgccgccg cgccatcgcg aatggaagcg cgccaaaatc gccatctgga tcgtgctggc
     1261 gctgctcgcc gtgggcgcgt tgcgtaccgt gatcgcggat atcatgcaaa gccgctcggt
     1321 cgccgacacg acgaagcaga acgccacgca atatgtcaac gtggtgagtc cgacgcaaac
     1381 ggaagatggc ggcaacaccc tgttgccggg caccttgcgc ggcaatgtcg aatcgccgat
     1441 ctacgcgcgc tccaacggct atctgctgca ctggtatgcc gatatcggcg cgcgcgtgaa
     1501 gcaggggcaa ctgctcgccg atctggatac gccggaaatc gatcaggaac tcgcccaggc
     1561 attggcgcag cgtcagcaaa ccagttcgag tctcggcctc gccaaaagct cgctggaccg
     1621 ctggcagcaa ctgcgtcaac gcgatgcggt ttcgcagcag gaactcgacg aacggcaaag
     1681 caccttcagc caggacgtcg ccaaccttgc cgccgccgat gccaacgtga aacgccttca
     1741 gcaactcgaa tcgttcaagc gcatcgtcgc gccgttcgcg ggtgtggtca cgcagcgcaa
     1801 cgtcgacgtc ggcgacctga tcgatgccgg cagcggcacg agccgcgcgt tgttcgcgct
     1861 ggcgcaatcg gacccgttgc gagtctatgt gcaactgccg caggcgtatg cgcaaaacgt
     1921 ttcggtcgga cagaaggtgg tggtcacgca ggccgaattg ccggaccagc agttccacgg
     1981 cacgatcacg cacatctccg gcgcgatcga cgtgccgacc cgctcgctgc aagtcgaagt
     2041 gaccttgccg aatcccgacg gcaagttgcg gcccggcgcg tatgtgcagg tggcggtgcc
     2101 ggcggttgcc catgcgcgcc tgacggtgcc gggtaatgcc ttgctgtttc gtgctgaagg
     2161 cccgcgtgtc gcggtggtgg atgcgaacgg caatgtggtg ttgcacaaga tcgtgatcgc
     2221 gcgggacctg gggcagtcgc tcgagatcga aagcggcatc gaggcgaacg acaaggtcat
     2281 catcaacccg agcgattcga tcgcggacgg cgatcacgtg cagattacgc agccgcagaa
     2341 aaacggcaag ggggcgtcgt gatcgcgaag caggcgggcg ctgatcgggc agccacgata
     2401 cggatggcac aaggggcgct ggcgtccgcc gcgagcgcgg ccttgctggc ggcgtgcaca
     2461 gtcggcccgg actatcagcg gccgcaagcc gaagtgccgc ccagctggca aaccgattcg
     2521 tactggcgcg tcgccgttcc gtcgcatgcg cccatttcgc cggactggtg gagcacgttc
     2581 cgcgatccga cgctcggcgc gctggaaacg caggcgctcg cacagaatca gacgctggcg
     2641 gcggctagcg cgcactacga gcagtccagg gccacgctcg cgaatacgcg cgcccagcag
     2701 attcccgaag tcgacgcgtt tgcggcggca tcgcgcttca gaatctccag tgaccgcccg
     2761 ctcacgaatt acgccacgcc gacgcagtcg accgtacaga acagtctgca actcggtccg
     2821 acgatcaact acgacaccga tctattcggc cggatacgcc gcgaggttga aagtgccgcc
     2881 gcctccacgc aacaatcggc cgacgacctc gccaacgcac ggctcgtcct gaccaccgat
     2941 ctcgccaccg actatttctc gctgcgtgag ctcgacgccg agatcgatgt gctgaatcag
     3001 tcggtgaaac tgcagcaaaa ggcgctcgat tacgtgacga ccgagcacga tctcggcgtg
     3061 gtctccggtc tcgacgtgtt gcagcagaag tcgctactcg attcgacccg ggtgcaggcg
     3121 caactgctgc tgaatcagcg cgcgcagttc gaacatgcga ttgccgcgct ggtgggcgtg
     3181 cccgcgccgc aattcgcgat cgagcccaag gtgctcgacg tgcaggtgcc gtcgatcccg
     3241 cttggactac cgagcgacgt cttgcaacga cgcccggata tcgcatcggc ggagcgcgcg
     3301 atggccgcgg cgaacgcgca gatcggcgtt gccaaggcgg ccttttttcc gagcctgacg
     3361 ctgaccccga gcatcggctg ggaaaccacc cagttcgcca gtctgctgag cgcgccgacg
     3421 ctgatgtgga cgctcggcgc ggccgtcggc caggtgctgt tcgacggcgg acgccgcgcg
     3481 gccaatgtcc agttcgcaag cgaaggctac aaggccaccg aggcaaccta ccggcaaact
     3541 gtgctcaacg cgttccagca ggtgcaggac ggcatcaccg gtctatccgt gctcgacggc
     3601 gcgtcgaagc agtcgcacga ggcggtgacc gacgcccagc atttgctggc gctcgccaac
     3661 gaccgctatt cgggcggcct tgcggcgtat ctcgacgtca tcaccgcgca acagtcgctg
     3721 ctcaccagcg agcgtcagga cgtgcagatc cacggccagc agatgacgtt gtcggtgtca
     3781 ctggtgaagg cactgggcgg cggttgggag gccggcacgg acgtttcggg cgcgcagccg
     3841 cgcggcaata caacgggcac ggcgaacgca gccaataccg cgaacgcagc caacaccgta
     3901 aacatagcga ccaccgcgaa catagctaac acgaaggaag cgatgactcc cgcgaagtca
     3961 gtcgctcgac aatgacagag ccagacagaa aacggaaaaa cgaggcgttt cgtataatgc
     4021 agtttgaaga ggatgcgtat gaaattgctg atcgttgaag acgaacacaa ggtggtggat
     4081 tacctgcggt ccggtttgac cgagcagggc tgggtcgtcg acgtcgcgct cgacggtgag
     4141 gaaggcatgc acctggccac cgaattcgat tacgacgtga tcgtcctcga cgtcatgctg
     4201 cccaaacgcg acggctttag cgtgctgaag gcgctgcgca tgcgcaagtc cacgccggtc
     4261 atcatgctga ccgcgcgcga tcacgtgaac gaccgggtgc gcggtctgcg cgaaggcgcc
     4321 gacgactacc tcaccaaacc cttttccttt ctcgaactgg tcgagcggct gcacgcactg
     4381 gcgcgccgca cgcgttcgca ggagtcgacg ctgatttccg tgggcgacct gtttgtcgat
     4441 ctgatcggcc ggcgcgctac tcgcgacggc gtacgcctcg atctgaccgc caaggaattc
     4501 cagttgctga gcgtgctggc gcgccggcaa ggcgacattc tctcgaagac cgccattacc
     4561 gaactcgtgt gggacgtcaa tttcgacagc catacgaacg tggtcgaaac cgccatcaaa
     4621 cgattgcgcg ccaagctcga cggcccgttt tcctcgaagc tgctgcacac cgtacgcgga
     4681 atgggctacg tgctcgaagt gcgcgaagag gcagagcaaa catgaaccgc tcgatcgcgc
     4741 gccgcctggc gtcgatgttc gcgctggtgg cgctgttcgt gttcacgctg atcggcactg
     4801 ggctgtttct ggtgttgcgc agccagctgg aacaccacct gcgcgaatcg ctcgacgatc
     4861 gcacccagat tgcgcgcatc atcgtctacc acgcggtgac gccggacaag tggcgcatgg
     4921 ctcgcgagaa gctcaccgac atgacgccgc acgacgccag caccgtgtat tcgatatcga
     4981 gcaccgatcc gtatttccac tatggcaatc ctgtcgacgg cacggtagtg aagcgctggc
     5041 cgggtggcta cgcgcgcgtg acgccgccgg gcggtgccga ggacatgctt acctccacgg
     5101 tgacgatacc ggcttacgcc aatcgcccgc ccgtgcagtt gcaggtggcc gcgagctatt
     5161 cgccgaacgt ccgcacgatg cgcgtgttcg gctcggcgct tgcggcgctg tcggcgctgg
     5221 gcagcctcgc cgtgctgctg ctcagttact cggtcacgcg gctcggcctc gcaccgttga
     5281 cgcgcctgac gagagatgcc tccgctgtga gcccgaataa ccgctcgcaa cgtctgaata
     5341 ccgcctcgct gccgctcgaa ttgaacgatc tggccaactc gttcaacggc gcgctcgaac
     5401 gtctggacgg cgcatacggc cgcctcgaat cgttcaacgc ggacgtggct cacgaactgc
     5461 gcacgccggt gacgattctg atcggccaga ccgaagtggc gttgacgcgc aaccgctcag
     5521 tcgacaattt gcggcagacg ctgcagtcga atctcgaaga gttcgaacgc atgcgcgcga
     5581 tcatcaacga catgctgttc cttgcccgcg ccgatcaggg cgagcgcgcg acggggctga
     5641 tcgaattgtc gctggcggcc gaagtcgcgc atacgctcga gtttctggag attccgctgg
     5701 aagaagcgcg ggtgtccgcg cagttgcgcg gcgatgcggt gacgcgtgtg aaccgctcgc
     5761 tgttcggccg cgcctgcgcg aatctgttga tgaacgcgat ccagcactgc gagccaggcg
     5821 cggaaattac cgtgacgatc acgcgtgacg cggatcagtt gcgagtcgcg gtggccaatc
     5881 ccggcgcgcc gatcgagccg gcggtgctcg aacatctgtt cgtccggttc tatcgcgccg
     5941 aagagtcgcg caccaatagc cgcgaaaacc acgggctggg gctcgcgatc gtgaaggcga
     6001 ttgcggagat gcatcgcggc acggtgtcgg tggaaagcgc gaacggcgtc aacacgtttg
     6061 cgttttctgt cgccgcgttg cgcgcggagg gcgacagccc ggcggcgcca cgttcgagtg
     6121 ccggtgtgac ggctgaggca ggcggttact cgtcgctcgt aaagggcaaa tcagcctgaa
     6181 ccggtcgacg ctgcttcacc ggctgcgcat tgtccggcgt caacgcgctt acgcgcaggc
     6241 ccaggagccg cagcttgcgg gtcaattcca cccgtctgag acattcggtt gccgcgcgcc
     6301 gtatttcggt ggcatcggcg gtcggttcgt ccagcgtcag gtcgcgcgtg accgtgcgga
     6361 agtcgtcgta gcgcagcttg atgcccaccg tgcggcccac gtagcccttg cgaaccagat
     6421 cttccgcgac ccgcacgcat aagcgcgtaa acgaagtcga ccagggtacg tgctaaaaat
     6481 ccagcgtgaa ctgcggtgac tcgtcgc
//
LOCUS       DATASH010000783         3735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3887420,
            whole genome shotgun sequence.
ACCESSION   DATASH010000783 DATASH010000000
VERSION     DATASH010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3887420"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..1576)
                     /locus_tag="VHV99_20995"
     CDS             complement(125..1576)
                     /locus_tag="VHV99_20995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEX3637911.1"
                     /translation="MFHRPTAPFLLTLLASVCALSACTVGPDYAGAPQVAPDAAHASS
                     FVRTPAVGMVSTRAPSQWWLSLDDPQLNTLIDAALAHNPDVHAAQARLREARAQLQHQ
                     KANGMPTASFDAAALRVREPNLSSFSSSGSSSSGGGPLQLYTAGFDASWEIDLFGGTR
                     RAVEAATAEADAVDADLADTQVSLAAEVAQAYVDLRDEQQRLTLAQHSAELEQQMLTL
                     TQQRRAHGTAADVDVERLTTQVENTRATLTPLDEQVAESLDQLAVLTGQAPGALDQEL
                     SAQRPLPAVPASVAIGDPASMLQQRPDIRAAERRLASSNAQIGEHKADFFPKVTLFGD
                     IGYTAADPGHLVRKNNFSWIGAPYLQWNVLDFGRTLSSVRGAEASRDEADAKYAHTVL
                     AALQDANTSLSRYGHQREHLATLQQVQASADRSLILVRQRYTAGTSSLIDLLDTQRTQ
                     FSAQQNVVSGQAELLKDFVSLQKSLGLGWRVQG"
     gene            complement(1597..3198)
                     /locus_tag="VHV99_21000"
     CDS             complement(1597..3198)
                     /locus_tag="VHV99_21000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011658318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEX3637912.1"
                     /translation="MADATTAAASLPAAPAPEEERASAADWLAVAAGSLGALMATLDI
                     SITNSALPQIQGEIGATGTEGTWISTGYLMSEIVMIPLAAWLTRVFGLRNFLLANATL
                     FIAFSMMCGWSHSLPMMIAGRIGQGFAGGAMIPTAQTIIRTRLPRSQMPVGMTMFGLI
                     VLLGPLLGPVLGGWLAENISWSWCFFINLPVCIALITLLIVGLPSDRPQWSTFFKADW
                     VGIIGLTIGLSSLTVVLEEGQRERWFESSTIISLTCVSLAGMALIAISQLAAKRPIMR
                     LSLMRNPRYASVIVIVFAVGAGLYGVSYLLPQFLGIVSGYNAQQSGAIMLISGVPAFL
                     IMPILPRILGKLDFRMLVIGGLLLFALSCMLDIDLTAQSVGHDFFWSQLIRGFAQILA
                     LMPLNQASMAAVSREDSGDAAGLYNMARNLGGSVGLAVIGTVIDRRTTFHTAMIRESL
                     TANSLVGQERLTSSAASWFAQTGDMAYSKMRALGQIAAQIHMQATVITYSETFYLLGL
                     ALLACVPLALLLKTPRGPVSSSAGH"
     gene            complement(3191..>3735)
                     /locus_tag="VHV99_21005"
     CDS             complement(3191..>3735)
                     /locus_tag="VHV99_21005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEX3637913.1"
                     /translation="QIAATTAQIAQARAQLEAAEASARQAHLDLQDTVVRSVLAGKVG
                     DRSVRVGQYVQPGTRMLSVVPVQSTYLTANFKETQVGHMRVGQPVILHVDALPGTDLH
                     GVVDSFAPGTGAQFALLPPENATGNFTKIVQRIPVRIRIETGPETRSVLLPGLSVTAD
                     VDTRSSREGDERIKAENNHG"
BASE COUNT          724 a         1181 c         1180 g          650 t
ORIGIN      
        1 ccgtcacccg tcacccgtca cccgtcaccc gtcacccgtc acccgtcacc cgtcatttac
       61 catgttcgaa atttttcgtg ctgcctcaat gcaatcctgc ggcgatatcc tcaaagcgct
      121 cgcgtcaccc ttgcacgcgc cagccgagtc ccaggctctt ctgcaaagag acaaaatcct
      181 tgagcaattc agcctgtccc gagaccacat tctgctgcgc gctgaactgc gtgcgttggg
      241 tatcgagcag atcgatcagg cttgatgtac ccgctgtata acgctgacgc accaatatga
      301 gcgagcggtc cgccgacgct tgcacttgct gcagcgtagc cagatgttcg cgctgatgcc
      361 cataacgcga cagcgaagta ttcgcgtcct gcaacgccgc gagtaccgta tgcgcgtatt
      421 tcgcatcggc ctcgtcgcgc gatgcttcgg cgccgcgaac gctcgagagc gtgcggccga
      481 agtccagcac gttccactgc agataaggcg cacctatcca gctgaaattg ttcttgcgca
      541 caaggtggcc cggatcagcg gcggtatagc cgatgtcacc gaacaacgtc accttcggga
      601 agaagtcagc cttgtgctcg ccgatctggg cgttgctcga agcaaggcgt cgttccgccg
      661 cgcgaatatc gggccgttgc tgcagcattg aagcggggtc accgatagct acggacgccg
      721 gcacggccgg taacggccgc tgcgcagaga gctcctggtc aagcgcgccc ggagcctgtc
      781 ccgtcaagac cgcgagctga tcgagcgatt cggcgacttg ttcgtctagc ggcgtcaacg
      841 tcgcgcgggt gttctcgacc tgcgtggtca ggcgctccac atcgacatcg gccgccgtgc
      901 cgtgcgcgcg gcgctgctgc gtcaacgtga gcatctgctg ctcgagttcc gcggagtgtt
      961 gtgcaagcgt caggcgttgc tgctcgtcgc gcaggtcaac gtaagcttgc gcgacttccg
     1021 ctgcgagcga cacttgggtg tcggcgaggt cggcgtcgac tgcatccgct tctgccgtcg
     1081 cggcttccac cgcgcgtcgc gtgccgccga acaaatcgat ttcccacgat gcatcgaagc
     1141 ccgccgtata gagctgcagc ggtcctccgc cggacgacga tgaaccgctc gaactgaacg
     1201 aactcaggtt gggctcgcgc acgcgcaacg cggcggcgtc gaaagaggcg gtgggcatgc
     1261 cgttcgcttt ttggtgctgc agttgcgcgc gggcttcgcg caaacgcgcc tgcgccgcgt
     1321 gaacgtccgg gttatgggcg agcgcggcgt cgatcagcgt gttcagttgg ggatcgtcga
     1381 gcgacagcca ccattgactc ggtgcgcgtg tcgagaccat gccgaccgcc ggcgtacgga
     1441 cgaagctgga tgcatgggcc gcgtcgggcg cgacctgggg cgcgccggca tagtcggggc
     1501 cgacggtgca ggccgagagt gcgcagacgg acgccagcag cgttagtaaa aagggagcag
     1561 tagggcgatg gaacatcgtg gatagtgtct ttgccgtcaa tggccggcgg acgacgaaac
     1621 ggggccgcgc ggagtcttca ggaggagcgc aagcggcacg caggcaagca acgccagacc
     1681 gagcaaatag aacgtttcag agtacgtaat gacggtagct tgcatatgga tctgtgccgc
     1741 gatttggccg agcgcacgca ttttggagta ggccatgtcg ccggtttgcg cgaaccagct
     1801 cgcagcgctc gacgtcaggc gctcctggcc caccagcgag ttggccgtca gcgactcgcg
     1861 gatcatcgcg gtatggaagg tcgtgcgccg gtcgatcacg gtcccgatga cggcaagccc
     1921 aaccgagccg ccaagattgc gggccatgtt gtagaggccg gctgcatcgc cggaatcttc
     1981 gcgcgagacc gctgccatgg acgcctggtt caacggcatc agcgcgagga tttgcgcaaa
     2041 gccgcgtatg agctgggacc agaaaaagtc gtggccgacg ctctgcgcag tcaggtcaat
     2101 atcgagcatg cagctcaacg cgaacaacaa cagtccaccg atcacgagca tgcgaaagtc
     2161 gagcttgccg agaatgcgtg gcaggatcgg catgatgaga aacgccggca cacccgatat
     2221 caacatgatg gcgcccgatt gctgcgcgtt atatccggac actattccga ggaactgcgg
     2281 cagcaaatac gacacgccat acagccctgc gccgaccgcg aacacaatga caatgacgct
     2341 tgcgtaacgc ggatttcgca tcaggctcag acgcatgatc ggccgcttcg cggcgagttg
     2401 cgaaatggcg atcagcgcca tgcccgccag cgacacgcag gtcagcgaga taatcgtgct
     2461 ggactcgaac cagcgttcgc gttgaccttc ttcgagcacc acggtcaacg aactgaggcc
     2521 gatcgtgagg ccgataatgc cgacccagtc cgccttgaag aacgtactcc actgcggccg
     2581 gtccgacggc agtccaacga tcagcaaggt gatcagcgca atacagacag gcaggttgat
     2641 aaagaagcac cagctccagc tgatgttctc tgccagccag ccaccgagca ccggccccag
     2701 cagcggaccc agcaacacga tcaggccgaa cattgtcatg ccaacaggca tctgcgagcg
     2761 tggcaaccgg gtgcgaatga tggtctgcgc cgtcgggatc attgcgccgc ccgcgaagcc
     2821 ctggccaatg cgcccggcaa tcatcatggg caagctgtgc gaccatccgc acatcatgga
     2881 gaacgcgata aacagcgtgg cattcgcgag caggaagtta cgcaagccga acacacgcgt
     2941 gagccatgcg gcgagcggga tcatcacgat ctcggacatc aggtagccgg tcgagatcca
     3001 cgtaccctcg gtcccggttg caccaatctc gccctggatt tgcggcaagg ccgaattggt
     3061 gatcgagata tccagcgtcg ccatcagcgc gccgagcgag ccggccgcaa ccgcgagcca
     3121 gtccgccgcg ctcgcgcgct cctcctccgg cgcgggggca gctggcaagg aagcagctgc
     3181 cgtggtcgca tcagccatgg ttgttctcag ctttgatgcg ctcgtcgcct tcgcgcgagg
     3241 agcgtgtatc gacatcagcg gtcaccgaca aaccgggcag cagcacgctg cgtgtttccg
     3301 gccctgtctc aatgcgaatg cgcacgggaa ttcgctggac gatcttggtg aaattgcccg
     3361 tggcgttttc cggcggcagc agcgcgaact gggcaccggt gccgggggca aaactgtcca
     3421 ccacaccgtg caggtccgtg cctggcaacg catccacgtg caggatcacc ggctggccga
     3481 cgcgcatgtg gccgacctgc gtctccttga agttggccgt cagataagtg ctttgcaccg
     3541 gcactaccga cagcatgcgc gtgcccggtt gcacgtactg gccgacgcga accgagcggt
     3601 cgcccacctt gccggccagt acgctgcgca cgacggtgtc ctgcaggtcg aggtgtgctt
     3661 gccgcgcgct ggcctccgct gcctcgagct gggcacgcgc ctgcgcgatc tgcgcggtgg
     3721 tggcggcgat ctgtg
//
LOCUS       DATASH010000784         2342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5271444,
            whole genome shotgun sequence.
ACCESSION   DATASH010000784 DATASH010000000
VERSION     DATASH010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5271444"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..1067
                     /locus_tag="VHV99_21010"
     CDS             63..1067
                     /locus_tag="VHV99_21010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX3637914.1"
                     /translation="MNLNSRRHPVARKIVSMCVAASAVWCASASQAADQPVVGLITKT
                     DTNPFFVKMKQGAEAAASKDGAKLITAAGKFDGDNASQVTAIENMMTAGAKAILITPS
                     DTKAIVPSIKKARAAGVMVVALDTPTDPQDATDALFATDNFKAGVLIGKYAKAAMNGK
                     PAKIATLDLAPGVSVGVLRHNGFLEGFGVKEGDPSIVCSQDTRGDQAKGQTAMENCLQ
                     KSPDINVVYTINEPAAAGAYRALKAAGKDKSVMIVSIDGGCEGVRNVKASAIAATSQQ
                     YPLKMASLGVTAGVEYAKTGKKVSGYQDTGVTLITDKPMSGVDSKDTKFGLDNCWGNK
                     "
     gene            1188..2174
                     /locus_tag="VHV99_21015"
     CDS             1188..2174
                     /locus_tag="VHV99_21015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3637915.1"
                     /translation="MSTPSVPAGHSRTFAERLPSLAEIGPLIALVLACGFFISQSNRF
                     LSFQNLSLILQQTMVVAVIAIGQTLIVLTGGIDLSCGMVMAFGSIIMTKFAVTLGVPP
                     ILAILCGIAASTLFGALNGVLITRIKLPAFIVTLGTLNIAFALTQIYSNAESVSNLPD
                     AIMFFGNTFKLGPADVTYGTVLTLLMYLATWFVLRDTVPGRHLYALGNNPEAARLMGL
                     SSQKILLIVYSLAGAIYGIAALLSVSRTGVGDPQAGQTENLDSITAVVLGGTSLFGGR
                     GSIVGTLLGALIVGVFRNGLTLIGVSSVYQVLITGMLVILAVAADKLSHRRG"
     gene            2194..>2342
                     /locus_tag="VHV99_21020"
     CDS             2194..>2342
                     /locus_tag="VHV99_21020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEX3637916.1"
                     /translation="MSTPTTASAVMPVLQARGLVKRYGNVTALDGCDFEVLPGEILAV
                     IGDNGA"
BASE COUNT          428 a          753 c          728 g          433 t
ORIGIN      
        1 cgttcgcgtg cgtcaccgcc cgcagttcac aacgtcgtgg ccaataaccg taggagcgtc
       61 ccatgaatct gaattcccgc aggcatcctg tcgccagaaa aatcgtctcg atgtgcgtgg
      121 cggcatcggc cgtgtggtgt gcgagtgcga gccaggcggc cgaccagccc gtcgtcggcc
      181 tcatcaccaa gaccgacacg aacccgttct tcgtgaagat gaaacagggc gccgaagcgg
      241 ccgcctcgaa agacggcgcc aagctgatca ccgcggccgg caagttcgac ggcgataacg
      301 cgagccaggt caccgccatc gaaaacatga tgacggccgg cgcgaaagcg attctgatca
      361 cgccgagcga taccaaggcg atcgtgccga gcatcaagaa agcgcgcgcc gccggcgtga
      421 tggtggtcgc gctcgatacg ccgaccgacc cgcaagacgc caccgacgct ctcttcgcca
      481 ccgacaactt caaagccggc gtgctgatcg gcaaatacgc gaaagccgcc atgaacggca
      541 agccagcgaa gatcgccacg ctcgatctcg cgccgggcgt atcggtcggc gtgctgcgtc
      601 ataacggctt cctggaaggc ttcggtgtga aggaaggcga tccgtcgatc gtctgcagcc
      661 aggacacccg cggcgatcag gccaagggcc agacggcgat ggagaactgc ctgcagaagt
      721 caccggatat caacgtggtc tacacgatca acgaaccggc cgcggcgggc gcgtatcgcg
      781 cgctcaaagc ggcgggcaag gataagagcg tgatgatcgt ttccatcgac ggcggctgtg
      841 agggggtccg caatgtgaag gccagcgcga ttgccgcgac ctcgcagcag tatccgttga
      901 agatggcctc gctcggcgtg acggcgggtg tcgagtatgc gaagaccggc aagaaagtct
      961 ccggctatca ggacacgggc gtgacgctga tcacggacaa gccgatgtcg ggcgtcgaca
     1021 gcaaggatac gaagttcggc ctggataact gttggggcaa taagtaacac gcgttgagtg
     1081 gcggcccgct gctctgggcc gccctcggat caccgggtgc cggatcgccg gccgccgtat
     1141 tcgccacgtg gccagcgcgc cacgctcatg catcgaggac acctgtcatg tcgactcctt
     1201 ccgttcccgc cggccactcg cgtaccttcg ccgagcgctt gccgtccctc gccgaaatcg
     1261 gaccgctgat cgcgctggtg ctggcgtgcg gcttcttcat ttcgcagagc aaccggttcc
     1321 tgtcgttcca gaatctctcg ctgatcctgc aacagaccat ggtggtcgcc gtcatcgcca
     1381 tcggccagac gctgatcgtg ctgaccggcg ggatcgatct gtcgtgcggg atggtgatgg
     1441 cgttcggctc catcatcatg accaaattcg cggtcacact cggcgtgccg cccattctcg
     1501 cgattctgtg cggcatcgcc gcgagcacgc tgttcggcgc gctcaacggc gtgctgatca
     1561 cacgcatcaa actgccggcc tttatcgtca cgctgggtac gctgaacatc gcgttcgcgc
     1621 tcacgcagat ctattcgaat gcggagagcg tctcgaacct gccggacgcg atcatgttct
     1681 tcggcaacac attcaagctc ggccccgccg atgtcaccta cggcacggtg ctcacgctgc
     1741 tgatgtatct cgccacatgg ttcgtcttgc gcgacacggt gcccggccgg catctgtacg
     1801 cgctcggcaa caaccctgaa gcagcgcgcc tgatgggcct ctcgtcgcag aagattctgc
     1861 tcatcgtgta ttcactggcc ggcgcgatct acgggattgc cgcgctgttg tcggtgtcgc
     1921 gcaccggcgt cggcgatccg caggccggcc aaaccgagaa cctcgacagc atcacggccg
     1981 tcgtgctggg cggcaccagt ctgttcggcg gacgcggctc gattgtcggc acgctgctcg
     2041 gcgcgttgat cgtcggtgtg tttcgcaatg gcttgacgct gatcggcgtc tcttcggtct
     2101 accaggtgtt gatcaccggc atgctggtga ttctcgcggt cgccgcggat aaactttccc
     2161 atcgtcgcgg ttgagtactg aacggagcct gtcatgtcga cacctactac cgcttccgcc
     2221 gtgatgcccg ttctccaggc gcgcggactc gtcaaacgct acggcaacgt caccgcgctc
     2281 gacggttgcg atttcgaagt cttgcccggc gagattctcg ccgtgatcgg ggacaacggc
     2341 gc
//
LOCUS       DATASH010000785         8416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1713281,
            whole genome shotgun sequence.
ACCESSION   DATASH010000785 DATASH010000000
VERSION     DATASH010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 8416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8416
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1713281"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(792..1538)
                     /locus_tag="VHV99_21025"
     CDS             complement(792..1538)
                     /locus_tag="VHV99_21025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbtA family protein"
                     /protein_id="HEX3637917.1"
                     /translation="MVGKLLVRGMLAGIAAGLLTFGFARVVGEPQVDQAISFEEKADA
                     AKGEAPEPELVSRGTQAGLGLLTGVVTYGAAFGGLFSLVFAFAYGRVGALSVRALSAW
                     LALGAFITLVIVPNIKYPANPPSVGDPETIGTRTGLFFLMIAISLAAMVFSLKVRRRM
                     ALMLGPWNGSIVAGVVFIVIIAAVQLSMPVINEVPAAFPAVLLWKFRVAAIGMQVIMW
                     TTIGLLFGAMVERSKLLAPANRSAAKSAYL"
     gene            complement(1550..1771)
                     /locus_tag="VHV99_21030"
     CDS             complement(1550..1771)
                     /locus_tag="VHV99_21030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbtB-domain containing protein"
                     /protein_id="HEX3637918.1"
                     /translation="MSNAVFDSATQPVAQPTPIPLRELLPWVVFGGLLLLLALYFVGA
                     EEGATSLVPGMYVHEFVHDGRHLLGFPCH"
     regulatory      complement(1814..1953)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2010..2584
                     /locus_tag="VHV99_21035"
                     /pseudo
     CDS             2010..2584
                     /locus_tag="VHV99_21035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490760.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
     gene            complement(2702..3142)
                     /locus_tag="VHV99_21040"
     CDS             complement(2702..3142)
                     /locus_tag="VHV99_21040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637919.1"
                     /translation="MNKTGILAAAIVAVSLSALLPCSANADDAPSPCASLKRIVAAAP
                     DGLASLTPDDGKGVAQPYGDDAQCSASRGSYQCTWTPHHDAGSSADALQAVAADIASC
                     LPDATHDQNSPGRQHFYLGTRAKRTDIALTRADSNKLRLVVSGK"
     gene            complement(3301..4314)
                     /locus_tag="VHV99_21045"
     CDS             complement(3301..4314)
                     /locus_tag="VHV99_21045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3637920.1"
                     /translation="MTQPLITDDERAPNERPVAVVASRHLSVATQAGSGTVPVFVNGD
                     WLAPARDVKRAVILIHGRLRNADTYFDLAQRACALAGGDAADTLLIVPQFLATADVGA
                     HALPPSTLHWEWTSWMGGGNAEGPAPLSSFDVLDAILQTLASREQFASLTEVVIAGHS
                     GGGQVVQRYAVVTQGEAPLVARGIAPRYVVANPSSYVYFDAMRPTASGEFAAFDAAAC
                     PSFNRWKYGLEDLPAYASGQGGAGSADALESRYARRDVTVLLGGADCDPQHPALDRSC
                     AAQTQGEHRLGRGLAYARYMASRHPEAAAMHRTFVIDGVGHDAKGIVASAHGLAALFG
                     VGR"
     gene            complement(4368..5723)
                     /locus_tag="VHV99_21050"
     CDS             complement(4368..5723)
                     /locus_tag="VHV99_21050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3637921.1"
                     /translation="MSEASLNQRDGVVSTTEASNGLSPSMVRRAIFASVLGNGLEWFD
                     FLIYGYFAKIIAQVFFPGGGFVSIMLTLATFAVGFIVRPIGGILLGIYADRAGRRKAL
                     SLLIISMAASTLLMGLTPAYASIGIAAPLLVVLARLLQGLSVGGQFATASAMLVEYAP
                     PHKKMFYGSFNMSAQAFALLLSSGVGYLLTTQLTHDQLVAWGWRLPFLFGALAGPFGF
                     YIRHRVAESPEFERLLDHKDKPPRVTVRQFFRDNGDAAICAMGVIIVGAATNYVWHSY
                     LSVYVERQLHLQLSTALLGAFVSGVLNLFLFPLSGKLADRYGAYRLFYPIVIAWMVCV
                     YPLYHFVVTNPTPGHLFIAQMVATVFLAAMSGSHPGMLATLFPVRSRSAGVALSYNIA
                     VTLFGGMAPLTITGLTHVTGSSLTPAFYLIFAGFVSLAMVYFTRSGREPGAEPAKFVT
                     R"
     gene            complement(5720..6796)
                     /locus_tag="VHV99_21055"
     CDS             complement(5720..6796)
                     /locus_tag="VHV99_21055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3637922.1"
                     /translation="MTFNLSRRLAVGGAIAGAAMLALLTQPAFAVHAHQPHLKPVATV
                     ADTRFTIDTPQGHAEFPLYLSKDWNVAQPQVTRAVIVIHGKLRNADVYFRTAQNARDA
                     AHAEADSTLLIAPQFLATRDLRVHDEPADLLRWRGNAWMGGEAAQAPLPISSYAVLDA
                     IVTRLADRRLFPNLRHVVFAGHSGGGQVVQRYAAAARNIAVLTAEGIDVRYLVASPST
                     YAYFDAQRPNAQGLAAPFDAAQCADFNQWKYGMDNRPPYLDDRTPAQLEATYASRRID
                     YLVGGADDDPQQSALDKTCAAEAQGPQRVARAEAYYRYMQSRHPEGLKQSFHIVPGVG
                     HNGARMLTSVCALSAMFDTKGCEQ"
     gene            complement(6897..7193)
                     /locus_tag="VHV99_21060"
     CDS             complement(6897..7193)
                     /locus_tag="VHV99_21060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637923.1"
                     /translation="MKKQCAVAALAEVSCATAHAQASVTLYGVIDTNIEFLNHAGADV
                     RFKRMGVPGADYILSKRADVYATVAYAASTRYANHQYTPVGLTSDTASVRTRPA"
     gene            7394..8302
                     /locus_tag="VHV99_21065"
     CDS             7394..8302
                     /locus_tag="VHV99_21065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3637924.1"
                     /translation="MELRHLRYFLAVAEEGQFTRAAERLAMQQPPLSQQIRTLEEEIG
                     FELFVRMPRGVTLTPAGQAFAEDAQRLLQDLQQSVDKAHRIARGELGTISIGLTSSAA
                     FHPFTTEAIRAFRAVCPEVAVELAELNAAEIIERLAAGQIQAAFLRKPVDAREGVAFE
                     LLLDEPMVVVLPVGHPLLKGAGRKRPQVSLKALAQEDFILVRRPGAPGMYADILAACR
                     QAGFVPRIAREVPRMVSGINLVATGLGVTLVPASMQRYDQVGTVYCTLTNPAKLSAPL
                     HLAYPAGLRNSAATRFIELVKERVRR"
BASE COUNT         1754 a         2719 c         2513 g         1430 t
ORIGIN      
        1 caccctataa ggaagactcc atcgcgggaa tccgacaaat cctaaactga cgacttcctt
       61 ggcctctcac aaccgaggct gtcgacacgg tcagcacaac ccgtgagaaa tcacgaagga
      121 ccatgtagtc agccaacgag cgacgacggc aacgagaaaa gcgcacgcag aaaagtcctc
      181 gtgactaatc cggtcacgag aaatttctgt gcaaaagtag atggaggcta agaggccgga
      241 tcgggccgag aagaacgaga ctgtgtgtct acgtgataac gaagcgacgc gtcagccgct
      301 agcgttcgag cgaacaccaa agagttatcc ccggtttttg ttcgcaaggc tgtggataac
      361 gttccgacaa cagccctaag tatttgatcc acttagattc ctgcacgctg cgcgcgataa
      421 caacgcgcct accactacac gcagttcacg cgccgtgaca tgggatgcac cccggcttcg
      481 ggcaaccaag cgattcgcat atccttatgc gaccggggaa cttgtccaca gaaaacgttc
      541 acaagcctgt ggataacctt ctggaaagcg cgccaagtac ttgatgtatt gggcttctct
      601 cgtactgcac gtcggcgtga ccgctcgcgc ggtcgcgcct ttacgtcggg cgtaatcgga
      661 cttcgccgcg gcgtttgcgg ccgctcttct gcatcaaaaa gtaggcacct tcgccgcggc
      721 tttctcggcc gcgccctttg catcaatagt aagcggactc cgtcgcggtc ttcacggccg
      781 cgcctctcac atcaaaggta agcggatttc gccgcgctgc ggttcgcggg cgccagcagc
      841 ttgctccgct caaccattgc gccgaacagc aacccgatgg tggtccacat aatcacctgc
      901 atcccgattg ccgcgacgcg aaacttccac aacagcactg ccgggaaagc cgcgggcact
      961 tcgttgatca ccggcatcga caactgcacg gcagcaatga tcacgataaa caccacaccg
     1021 gccacgatcg agccattcca cgggcccaac atcagcgcca tgcggcgccg tactttcagc
     1081 gaaaacacca tcgcggccaa cgagatcgca atcatcagaa agaacaagcc ggtccgcgtc
     1141 ccgatcgttt cgggatcacc caccgaaggg ggattcgccg ggtatttaat gttcgggacg
     1201 atgaccagtg tgataaacgc gccaagcgcc agccacgcag acaacgcacg cacactcaac
     1261 gcaccaacgc ggccatacgc aaacgcgaac accaatgaga acagcccgcc gaaggcggcg
     1321 ccgtacgtca ccacgccggt caacaatccg agccccgctt gcgtaccgcg gctcaccagc
     1381 tcgggttcgg gcgcttctcc tttggcggcg tcggcctttt cctcgaagga aatcgcctga
     1441 tccacctgcg gctcgcccac cactctggcg aagccgaacg tgagcaatcc cgcggcgatg
     1501 cccgcaagca tgccgcgtac caacaattta ccaaccatgt tcgactccgt tagtgacagg
     1561 gaaagccgag cagatggcgg ccgtcatgca cgaattcatg cacatacatg ccaggcacga
     1621 gcgacgtggc gccttcttcg gctccaacga aatagagcgc gagcaacagc agcaagccgc
     1681 cgaacacgac ccaaggcagc aactcacgca gcggaatcgg cgtgggttga gcgacgggct
     1741 gggtggcgga atcgaaaaca gcgttggaca taatggacat ctcctggggg taacgcgccc
     1801 cgatagtcga ttgaagagga atgctgcgaa gctcaggtct ggctttcggc ttgtgaatgc
     1861 cgattacagt ggcgcgaccg cgccgggctc tcaccggctt ccgtgtttcg cagtgtcgct
     1921 attctacgcg ctaaactcga tgtcccggct acgcagagca tgcgccgcat gcgtcctgct
     1981 ttagtcgggc ctccatggaa atcaaccgaa tggacacaag gctgttactg atcagccacg
     2041 cagcgaccgc ggcgatgcgc gcgggccgct tccccgccga cgatccgctc gacgcacgcg
     2101 gcttcgccgc agcaggcgcc gcgcgtgcgc gcctgtcgat tccggaggac gcggcggctc
     2161 tcgtcagccc ttcggtgtgc gcgcgcgaaa cggcctcggc cttgggtctc gcgcggcggt
     2221 ccacgaggga ctggcggata tcgactacgg tctatggcat ggccgacgcg ttgccgatct
     2281 cgccatcgaa gcgccgcaag atctcaccgc atggacacac gatcccgacg ccgccgcgca
     2341 cggcggcgag tccttcagtc ggctcgtgaa acggatagga cagtggctcg atgcgttgga
     2401 cgaagcaccg cgcgatacgc aaaatgtcgt agccgtcacg cacgcaccgg tgatccgagc
     2461 cgccatcgtt catgcactcg gggcgtcgcc gcaggtgttt tcgcgcatcg agatcgcgcc
     2521 gctttcgatg gtcgagttgc gacgcacgcg acgcggttgg acatggtggc ccgcgtcacc
     2581 ctagcagtcg cagggaagtc ttcccccctt tttcgaggaa gtacgatgac aataaattaa
     2641 tggcccaggt gggtatggtg cgccgcgcac ggacaaccga ttcgaacccg cgtgtgctga
     2701 cttatttccc cgatacaacc aacctcagct tgttcgaatc agcccgcgtc aacgcgatat
     2761 cagtccgctt ggcccgcgtc ccgagataaa aatgctgacg tcccggcgaa ttctggtcat
     2821 gcgtcgcatc cggcaggcac gacgcgatgt ccgccgcgac agcctgcaac gcatccgcac
     2881 tggaaccggc gtcatggtga ggcgtccacg tacattggta actgccacgg cttgctgaac
     2941 attgcgcgtc gtcgccgtag ggctgcgcga cgcccttgcc gtcgtccggc gtcagcgagg
     3001 ccaaaccgtc cggcgccgcc gcgacgatcc gtttaagcga ggcgcacggg ctaggcgcat
     3061 cgtctgcatt cgcgctgcac ggcagcaacg cactcagcga tacagcgaca atcgcggcgg
     3121 cgagtattcc ggtcttattc atgtcggcgc agtccttcga aaggtggctc acagatggca
     3181 ttagcgcggt agcacgcttc gtgcccatgc acaccggcga ctcacgcctt cgtcggcgaa
     3241 gtccatagcc tgcgcatagc cctgcgccaa acgcatcgac aagcgcttta agagctcact
     3301 tcaacgcccg acgccgaaca acgcagccaa cccatgcgcc gatgcaacaa tccccttcgc
     3361 atcgtgccca acgccgtcga tcacaaacgt acgatgcatc gccgccgctt ccggatgcct
     3421 cgaagccata taacgcgcgt aagccagccc ccgcccgagc cgatgctcgc cctgcgtttg
     3481 cgcggcgcat gaacgatcga gtgccggatg ctgcggatcg caatccgcgc cgccgagcag
     3541 cacagtgacg tcacggcgcg catagcgcga ttcgagcgca tcagcggaac ccgcgccgcc
     3601 ttggcccgaa gcataagccg gcaaatcctc gagcccatac ttccagcgat taaacgacgg
     3661 acaagccgcc gcatcgaacg cggcgaattc gccggacgcg gtcggccgca tcgcatcgaa
     3721 atacacatac gacgaaggat tcgcgaccac atagcgcggc gcgatcccgc gcgcgacgag
     3781 cggcgcctca ccctgcgtca ccacggcata gcgctgcacc acctggccgc cgcccgaatg
     3841 cccggcaatc accacctcgg tcagcgacgc aaactgctcg cgcgacgcca gcgtctgcag
     3901 gatcgcgtcg agcacatcga aagaactgag cggcgccgga ccttcggcgt tcccgccgcc
     3961 catccagctc gtccattccc aatgcagcgt ggacggcggc aatgcatgcg cgccaacgtc
     4021 agcggtcgcg agaaactgcg gcacgatcag cagcgtatcg gccgcatcgc caccggcaag
     4081 cgcacacgcg cgttgcgcca gatcgaagta cgtgtccgcg ttgcgtaagc gtccgtgaat
     4141 caggatcacc gcgcgtttga cgtcacgtgc cggcgcaagc cagtcaccat ttacgaacac
     4201 cggcacagtg ccgctgcccg cttgcgtcgc gacactcaga tgccggctgg caacgaccgc
     4261 gaccggacgt tcattcggcg cgcgttcgtc atctgtgatc aaaggctggg tcatgcaata
     4321 agctcgtaat taatctaatg agtctgatga gtggcgatgt agcgaatcta gcgcgtgacg
     4381 aacttcgcag gctccgcgcc cggctcgcgt cccgagcggg tgaaatacac catcgcgagc
     4441 gatacgaagc cggcaaaaat cagatagaac gccggcgtca aactgctgcc ggtcacatgc
     4501 gtcaagccgg tgatggtgag cggcgccatg ccgccgaaca gcgtcaccgc gatgttgtac
     4561 gacagcgcca cgcccgccga gcggctgcgc acgggaaaca gtgtcgcgag catgcccgga
     4621 tgcgaaccgg acatggcggc gaggaatacc gtcgcgacca tctgcgcgat gaacagatgc
     4681 cccggcgtcg gattggtcac gacgaagtga taaagcggat acacgcacac catccatgcg
     4741 atcacgatcg gatagaacag ccgatacgcg ccgtaccgat cggcgagctt gccggacagc
     4801 ggaaacagga acagattcag cacgcccgac acaaacgcgc cgagcagggc ggtggacagc
     4861 tgcaaatgca attgccgttc gacatacacc gacagataag agtgccaaac ataattggtc
     4921 gccgcgccga cgatgatcac acccatcgcg cagatcgccg cgtcgccgtt gtcgcggaag
     4981 aactggcgca ccgttacgcg cggcggcttg tccttgtgat cgagcaaacg ctcgaactcc
     5041 ggcgactcgg ccacgcgatg ccggatgtag aaaccgaacg gccccgccaa tgcgccgaac
     5101 agaaacggca gacgccagcc ccaggccacc agttggtcat gcgtaagctg cgtggtcagc
     5161 agataaccga caccggagga aagcagcaac gcaaaggcct gcgccgacat gttgaagctg
     5221 ccgtagaaca tcttcttatg cggcggcgca tactcgacca gcatcgccga cgccgtcgcg
     5281 aactgcccgc ccaccgagag cccttgcaac aggcgcgcga gcaccaccag caacggcgcc
     5341 gcgataccga tgctggcata agccggcgtg aggcccatca gcagcgtgct tgccgccatc
     5401 gaaatgatca gcagcgaaag cgctttacgc cgccctgccc ggtcagcata gatgccgagc
     5461 agaatgccac cgatcggccg cacgataaaa ccgacggcga acgtggcaag cgtcagcatg
     5521 atcgacacga agccgccgcc gggaaagaac acctgcgcga tgatcttcgc gaagtagccg
     5581 tagatcagaa aatcgaacca ctcgagccca ttgccgagca cggaggcgaa gatcgcgcgc
     5641 cgcaccatcg aagggctcaa gccgttcgat gcctccgtgg tggacaccac gccatcacgc
     5701 tgattcaagc ttgcctcgct cattgctcac atcctttggt gtcgaacatc gccgacaggg
     5761 cacagaccga cgtcagcatg cgcgcgccgt tgtggcccac gcccggcacg atatgaaagc
     5821 tctgcttcag gccttccgga tgacgcgact gcatatagcg gtaatacgct tcggcacggg
     5881 ccacacgctg cggaccttgc gcttcggcgg cacaggtttt gtcgagcgcg ctttgctgcg
     5941 gatcgtcgtc cgcgccgccg accaggtagt cgatccggcg cgacgcataa gtcgcttcga
     6001 gttgcgcggg cgtacggtcg tcgagataag gcggccgatt gtccatgccg tatttccatt
     6061 gattgaagtc ggcgcactgc gcggcatcga acggcgccgc cagcccttgc gcattcggcc
     6121 gttgcgcgtc gaaatacgca tacgtcgacg ggctcgccac cagatagcgc acgtcgatgc
     6181 cttccgcggt cagcacggcg atgttacgcg cggccgccgc gtaacgctgc acgacctgcc
     6241 cgccgcccga atgcccggcg aacacgacat gccgcaaatt tggaaacagc ctgcgatccg
     6301 cgaggcgcgt cacgatcgcg tccagcaccg cgtacgaact gatgggcagc ggcgcctgcg
     6361 ccgcttcgcc gcccatccac gcattgcccc tccagcgcag cagatcggcc ggttcgtcat
     6421 gcacgcgcaa gtcgcgcgtg gcgagaaatt gcggcgcgat caggagcgtg ctgtcggcct
     6481 ccgcgtgggc ggcgtcgcgt gcattctgcg cggtgcgaaa gtacacgtcc gcgttgcgca
     6541 gtttgccgtg aatcacgatc accgcacgcg tcacctgcgg ctgcgcgacg ttccaatcct
     6601 tcgacagata cagcggaaat tcagcgtgcc cttgcggcgt gtcgatcgtg aaccgtgtat
     6661 ccgcgaccgt cgccaccggc ttcagatgcg gctgatgggc gtgcaccgcg aaggccggtt
     6721 gcgtgagcaa ggccagcatt gcggcacccg ctattgcgcc gcccaccgcc agacgccggg
     6781 aaagattgaa tgtcatgcag cttgctcctg atcaccgtgc actgccttga acgcagcgca
     6841 tcgtcaaatc cgtcgtggaa agcccaaaac tcagaaccgg tggcgaatgc cgagcgtcac
     6901 gccggtctgg ttcggaccga agcggtgtcg ctcgtcaagc cgaccggcgt gtactggtgg
     6961 ttggcataac gcgtggaagc ggcataggca acggttgcgt acacatcggc tcgcttcgac
     7021 agaatatagt ccgcgccggg cacgcccatc cgcttgaacc gcacgtcggc gcctgcatga
     7081 ttgaggaatt cgatgttcgt gtcgatcaca ccgtacagcg tgaccgatgc ctgagcatgc
     7141 gcagtcgcgc aactcacctc ggccaaggcg gccaccgcac attgcttttt cactagcgtc
     7201 tccggtcggt tttgttttat gtcgcgatgc ccctgagcct gcttgccgtg ggcaacgccg
     7261 gcatcggtcg gtgcgatgga cgagatcgta gaagcgggcg tggcaaatgt aaaatacctg
     7321 ttttatgcat tatccatatt tatagaatat ggcagtgaat acaacgcaga gcacgaccga
     7381 ggagacagaa cacatggagc ttcgccactt gcgctacttc ctggcggtcg ctgaggaagg
     7441 ccagttcacc cgtgcagctg aacggctggc tatgcagcaa cctccgctct cgcagcagat
     7501 tcgaacgctg gaagaagaga tcggcttcga actgtttgtg cgcatgccgc gcggcgtgac
     7561 cttgacgccg gcagggcagg ctttcgccga agacgcgcag cgcttgctgc aggacctgca
     7621 acagtcggtc gacaaggcgc accggattgc acgcggcgag ctcggcacga tctcgatcgg
     7681 cctcaccagt tcggcggcgt tccatccctt cacgacggag gcgattcgag cctttcgcgc
     7741 agtctgtccc gaggtcgcgg tcgagcttgc cgagctcaac gcggcggaga tcatcgagcg
     7801 tttggctgcg gggcagattc aggctgcgtt tctgcgcaag ccggtcgatg cacgcgaagg
     7861 cgtggcgttc gagttattgc tcgacgaacc aatggtggtg gtgctgccgg ttgggcatcc
     7921 gttgctgaag ggcgcgggca ggaagcggcc gcaggtgtcc ttgaaggcgc tggcgcagga
     7981 ggacttcatt ctggtgcgcc ggcctggggc gcccggcatg tatgccgata tcctggctgc
     8041 ctgccggcaa gccggatttg tcccccggat tgcgcgcgaa gtgccgcgca tggtgtcggg
     8101 gatcaatctg gtcgcaacag ggttgggcgt aacgctcgtg ccggcgtcga tgcaacgtta
     8161 cgaccaggtg ggaacggtgt attgcacgtt gacgaatccg gcgaagctga gcgcgccgct
     8221 gcatcttgcc taccccgcgg gactgcgcaa tagcgcggca acccgcttta tcgaactggt
     8281 gaaggagcgc gttcgccgct gaatgcgccg ggacttttgc aacgatgccg caaaggcctg
     8341 gaaaccggtg aagccgtttg gcacagcaag atcggcgtgt gtaggcatac aaattgcgtt
     8401 cgaaccggac cggctc
//
LOCUS       DATASH010000786         3804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6063644,
            whole genome shotgun sequence.
ACCESSION   DATASH010000786 DATASH010000000
VERSION     DATASH010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6063644"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="VHV99_21070"
     CDS             <1..790
                     /locus_tag="VHV99_21070"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX3637925.1"
                     /translation="ELGVQDVVIVTLKAHSLPGVVDAMQPLLGKHTSIVTSVNGIPYW
                     YFYQHGGKFAGTRLASIDPDGSQWTKLGPERAIGCVLYPAAEIVEPGVIKHVYGKKFP
                     IGEPNGERTPRIQQLHEIMQAAGFDAPIRDNIRDEIWLKLWGNLCFNPISALTHATLD
                     VLTSDPGTRAVSRTMMLEAKRIADQFGVHFRVDVEKRIDGAGAVGAHKTSTLVDLENR
                     RPMEIDPLLTVVQEMGRLVAEPTPTIDVVLALIKLRERMALQGD"
     gene            complement(877..2328)
                     /locus_tag="VHV99_21075"
     CDS             complement(877..2328)
                     /locus_tag="VHV99_21075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX3637926.1"
                     /translation="METETDLRRHDLLIDGKRLPPGTGEYSIDINPATEEPIALVAQG
                     SATDVDTAVRAARAALKVWNAIRAAERGRILMRLAGLMRANLEELAALESVDAGKPIS
                     AVMRQDIPAAIDTLEYYAGWCDKINGQVVPVRPDALTYTLREPVGVVAAIVPWNFPLM
                     IGMWKIAPALACGCTLIVKPAEITPLTALRIGELALEAGVPPGVLNIVTGKGRVVGDA
                     LVAHPGIDKVTFTGSPSVGRGILQGAASNFKRVTLELGGKSANLIFPDANLDNAVRAS
                     ASGIFFNTGQVCSAGSRILAHRDVYDEVVERLAARAKAIKVGDPAASETSMGPLISAA
                     QMKTVLGYVETGRSEGASLVTGGARVGERGFFVEPTVFANVEHEMRISQEEIFGPVAT
                     VIRFNDEADAIRIANGTLYSLAAGVWSADIGRVHRVARDLKAGTVWINTYGYTDVRLP
                     WGGSGDSGFGREHGDVAIENFTEPKAVWLAIDQ"
     gene            complement(2406..3392)
                     /locus_tag="VHV99_21080"
     CDS             complement(2406..3392)
                     /locus_tag="VHV99_21080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3637927.1"
                     /translation="MNVSLQQLKVFVAVARERSFTRAAREFDLTQSAVSRCVRELEDA
                     VELKLFDRTTRQVELTNAGASLERRIGRLLDEIELTLREERAAYDGHTGVVVLASNPV
                     LSSSWVAQGLARCASAFPELIVSVKDQPQSGVLASVEQGEVDFGVVSVAAPLGGDQLH
                     AQVVFTTPLHVVMPPAHPLARQAGVEWRALHEWALVTLNADTGMRAALELAFNAHGLK
                     RRPVQELGHVAAVLRMVELGLGVGVLPVDAHWLTLGTSLVSRPLVPEMNLTTLLVHRR
                     NRSLRPNAAAAWAQLASLGGAPAPSAGDTASFPSLGFTSQAAAPGGAILHEP"
     gene            complement(3423..>3804)
                     /locus_tag="VHV99_21085"
     CDS             complement(3423..>3804)
                     /locus_tag="VHV99_21085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxalate/formate MFS antiporter"
                     /protein_id="HEX3637928.1"
                     /translation="GSNPYAFMTFAALIFLFWGEIFSIFPAICADTFGSKYAAANAGT
                     LYTAKGTASLIVPIASVLSAAGGWNLVFVVSAVVTIAAGISAKFILAPMRSRWIESHN
                     DPQGVLAAAGSKSSLSHWPEQTGE"
BASE COUNT          753 a         1252 c         1133 g          666 t
ORIGIN      
        1 cgaactcggc gtgcaggatg tcgtgatcgt cacgctaaag gcgcactcat tacccggcgt
       61 ggtcgacgcc atgcagccgc tcctcggcaa gcacacgtcg atcgtcacca gcgtcaatgg
      121 gattccctac tggtattttt accagcacgg cggaaagttc gccggcacgc gcctcgccag
      181 catcgacccg gacggtagcc aatggaccaa gctcggtccc gaacgcgcga tcggctgcgt
      241 gctgtatccc gccgcggaga ttgtcgaacc gggtgtgatc aagcacgtgt acggcaagaa
      301 attcccgatc ggcgagccga acggcgaacg cacgccgcgc attcagcaat tgcacgagat
      361 catgcaggcg gccggtttcg atgcaccgat ccgcgacaat atccgcgacg aaatctggct
      421 caagctgtgg ggcaacctgt gcttcaatcc gatcagcgca ttgactcacg ccacgctcga
      481 tgtcctcacc agcgaccccg gcacacgtgc ggtatcgcgc acgatgatgc tcgaagccaa
      541 gcgcattgcg gatcagttcg gcgtgcattt tcgcgtcgat gtggaaaaac gcatcgacgg
      601 cgcgggcgcg gtcggcgcac ataagacctc gacgctggtc gatctggaga accgtcgtcc
      661 gatggagatc gatccgctat tgacggtggt gcaggaaatg ggccgcctcg ttgccgagcc
      721 cacgccgact atcgacgtcg tgctcgccct gatcaaactg cgtgaacgga tggccttgca
      781 gggcgattga acgcgcattc acgctgcgag caacgcgtca tcagcttcac aaaaggatcg
      841 aacgccacgc gccgttcgat cctttctttt gcaccgctac tggtcgatcg ccagccacac
      901 cgccttcggt tcggtgaagt tttcaatcgc cacgtcaccg tgctcgcggc caaaaccgga
      961 atcgccggaa ccgccccaag gcaaacgcac gtcggtatag ccgtaggtat tgatccatac
     1021 ggtgccggct ttcaggtcac gcgcaacccg atgcacgcgg ccgatatccg cgctccatac
     1081 gccggccgcg aggctataca acgtgccatt cgcgatacgg atcgcatcgg cttcgtcgtt
     1141 gaaacgaatc acggtggcta ccggcccgaa gatctcctcc tgcgaaatgc gcatttcatg
     1201 ctcgacgttg gcgaataccg tcggctcgac gaaaaagcca cgctcgccca cgcgcgcgcc
     1261 gccggtcacg agcgacgcgc cttcggaacg ccctgtctcg acatagccga gcacagtctt
     1321 catctgcgcc gcggagatga gcggccccat cgacgtttcg ctcgccgccg gatcgccaac
     1381 cttgatcgcc ttcgcacgcg ccgcgagacg ctcgaccact tcgtcgtaaa cgtcgcgatg
     1441 agcaagaatg cgcgagcccg ccgaacacac ctggccggtg ttgaagaaaa tccccgacgc
     1501 cgacgcgcgc acggcattgt cgagatttgc atccgggaag atcaggtttg ccgacttgcc
     1561 gccgagctca agcgtgacgc gcttgaagtt gctggccgcg ccttgcagaa tgccgcgtcc
     1621 cacggaaggc gagcccgtga aagtcacttt gtcgatgccc ggatgcgcca ccagcgcatc
     1681 gccgaccacc cgccccttgc cggtgacgat attcagtact cccggcggca cgcccgcttc
     1741 gagcgcgagt tcgccgatgc gcaacgcggt tagcggcgta atctccgcag gcttgacgat
     1801 cagtgtgcaa ccgcaggcca gcgcaggtgc gattttccac atgcctatca tcaacgggaa
     1861 attccacggc acgatcgcgg cgaccacgcc gaccggttca cgcaacgtgt acgtcaacgc
     1921 atcgggacgc accggcacca cttgcccgtt gatcttgtcg caccagcctg cgtagtattc
     1981 gagcgtatcg atcgctgccg gaatatcctg gcgcatcacc gccgagattg gcttgccggc
     2041 gtcaacgctt tcgagcgcgg cgagctcttc cagattcgcg cgcatcagac cggcgagacg
     2101 catcaggatg cggccccgct cggctgcccg tatcgcgttc cacactttca atgcggcacg
     2161 cgccgcgcgg accgcggtat cgacatcggt agcgctgcct tgtgcaacca gtgcaatcgg
     2221 ctcttcggtc gcagggttga tgtcgatgga atactcgccg gtgccgggcg gcaaacgctt
     2281 gccgtcgatc agcagatcat ggcgtctgag gtcggtttca gtttccatcg tgaagctcct
     2341 tgtgtgcaat gccggatgaa ccgactggcg gcggatcggc cgaggccagt ggctcgagac
     2401 gaggcttagg gttcgtgcag gatcgcgcca cccggcgccg ctgcctgaga tgtgaacccg
     2461 agcgaaggga acgatgccgt gtcgcccgca gaaggcgcag gcgcgccgcc gagcgaggcg
     2521 agttgcgccc atgccgccgc ggcgttgggt ctgagggagc gattgcggcg atgcacgagc
     2581 agcgtcgtca gattcatttc gggaaccagc ggccggctca cgagtgacgt gcccagtgta
     2641 agccaatgcg cgtcgacagg taaaacgccg acgccgaggc ccaattcgac catgcgcaac
     2701 accgccgcaa catggccgag ttcctgcacc ggccgccgtt tcaggccatg tgcgttgaag
     2761 gcaagttcga gcgcggcccg catgccggtg tcggcattca acgtgacgag cgcccattcg
     2821 tgcaacgcac gccattcaac gccggcttgc cgcgccagcg gatgcgcggg cggcatcacg
     2881 acatgcagtg gcgtcgtgaa aaccacttgc gcgtgcaact ggtctccgcc aaggggcgcg
     2941 gccaccgaga ctacgccgaa gtccacctcc ccctgctcga cactcgcgag cacaccgctt
     3001 tgcggttgat ccttgacgga cacgatcagc tcgggaaatg ccgacgcaca acgcgcaagc
     3061 ccctgagcca cccagctgga cgagagcact ggattgcttg ccagcacgac tacaccggtg
     3121 tgcccatcat aagcggcgcg ttcttcacgc agcgtcagct cgatttcatc gagcaggcgg
     3181 ccgattctgc gctccagact cgcgcccgca ttcgtcagtt ctacctggcg cgtcgtgcgg
     3241 tcgaacagtt tcaattcgac cgcgtcttcg agttcgcgca cacagcggct cactgccgac
     3301 tgcgtcagat cgaactcacg tgccgcgcgc gtaaagctcc tttcacgcgc gacggcgaca
     3361 aacaccttga gctgctgcaa cgaaacattc atgacgtcac tgccaggcgt ggacctgtca
     3421 ccctattccc ctgtctgctc aggccagtga ctcagcgagg acttgctgcc ggcagccgcc
     3481 agcacgcctt ggggatcatt gtgcgattcg atccagcgtg aacgcatggg cgcaagaatg
     3541 aacttcgccg atatacccgc ggcgatcgtg acgacggcgg agacgacaaa gacaagattc
     3601 cagccgcccg cggccgacag caccgacgca atcggcacga tcagtgaggc cgtgcccttc
     3661 gcggtataaa gcgtacccgc attggccgcc gcatatttgc tgccgaaggt gtcggcgcaa
     3721 atggcgggga agatcgagaa aatttcaccc cagaacagga agatcaacgc ggcgaacgtc
     3781 atgaacgcat acgggttgct gccg
//
LOCUS       DATASH010000787        11652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1912419,
            whole genome shotgun sequence.
ACCESSION   DATASH010000787 DATASH010000000
VERSION     DATASH010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 11652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11652
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1912419"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2291
                     /locus_tag="VHV99_21090"
     CDS             <1..2291
                     /locus_tag="VHV99_21090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004537876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HEX3637929.1"
                     /translation="KAIENLKKLSPAPFGVKDDAQYIQPANYDDDIEKLAECDLVIEA
                     IAERMDWKHDLYKKVSPHIAPNAIFATNTSGLSITDLSNGFPDELKARFCGVHFFNPP
                     RYMHLVELIPTASTRPEILDQLESFLTSVVGKGAVRAKDTPNFIANRVGIFSILAVIT
                     EAAKFGLRFDEVDDLTGSRLGRAKSATFRTADVVGLDTMAHVIKTMQDTLKDDPFFPV
                     YETPAVLAELVKKGALGQKTGGGFYRKEGKAIKVLDPKTGGYVDGGAKADELVGRILK
                     RPPAERLKLLRESEHPQAQFLWSIFRDVYHYIGVHLESIADNARDVDLAIRWGFGWNE
                     GPFEGWQTAGWKQVAEWVQEDIAAGKALSNVPLPSWVLEGPVAEKGGVHTNEGSWSPA
                     SKTFVPRSSLSVYDKQVFRAPLVGETSADPKTYGKTLFETDAVRAWVDDRAGENDVLI
                     VSFKSKMNTIGPSVIDGLTQAIELAEKEYKGLVVWQPTSLKLGTPGGPFSAGANLEEA
                     MPAFMMGGAKGIEPFVRKFQQGMLRVKYASVPVISAVSGIALGGGCELALHSAKRVAH
                     IESYIGLVEVGVGLVPAGGGLKEAALRAAEAATQVGATNDLLKFVQKSFENAAMAKVS
                     ASALDARAMGYLKPSDTIVFNVFELLDVAKKEARALSAAGYRPPLRVTQVPVAGRSAI
                     ATIKASLVNMRDGRFISEHDFLIASRIAEAVCGGDVEAGSLVDEEWLLKLERRAFVDL
                     LGTQKTQERIMGMLQTGKPVRN"
     gene            2368..3567
                     /locus_tag="VHV99_21095"
     CDS             2368..3567
                     /locus_tag="VHV99_21095"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011353151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX3637930.1"
                     /translation="MAKQLQDAYIVAASRTPIGKAPRGMFKNTRPDELLVHAIRSAVA
                     QVPGLDTKVIEDAIIGCAIPEAEQGLNVARMGALLAGLPNSVGGVTVNRFCASGLTAL
                     AMAADRIRVGESDAMIAGGCESMSMVPMMGNKPSMSPHIFDRNEDIGIAYGMGLTAEK
                     VADRWKISREAQDAFSVESHRRAIAAQQAGEFNDEIAAYTITERFPDLATGEVKVKTR
                     EVSLDEGPRGDTSMEGLAKLRTVFASKGSVTAGNSSQTSDGAGALIVVSEKMLKEFNL
                     TPLARFVSFAVRGVPPEIMGIGPKEAIPAALKAAGLKIEDMDWIELNEAFAAQSLAVI
                     QDLGLDPSKINPLGGAIALGHPLGATGAIRASTVVHGLRRRNLKYGMVTMCVGTGMGA
                     AGIIERL"
     gene            3729..4499
                     /locus_tag="VHV99_21100"
     CDS             3729..4499
                     /locus_tag="VHV99_21100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017334171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX3637931.1"
                     /translation="MTMDILLERADGVLTIAFNRPDRKNAITAAMYQAMADALVEAQG
                     DTSIRVILIRGSAGIFSAGNDLEDFMKTPPMGETAPVFQFLRAISSAEQPVVAAVAGP
                     AVGIGTTLLLHCDLVYAADTASFSLPFTQLGLCPEAASSLLLQRVAGYQAAAEKLLLG
                     EAFDAAEAHRMGFVNRVLPAAEVDAFAAAQAAKLAALPASSLRVTKSLMKRASHQELH
                     TQMTEEAVHFGKMLLAPEAREAFKAFFEKRKPDFRSFP"
     gene            complement(4625..6025)
                     /locus_tag="VHV99_21105"
     CDS             complement(4625..6025)
                     /locus_tag="VHV99_21105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012400578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX3637932.1"
                     /translation="MKILLDASKGVSLAEQLTCQLEEMIRSRQVQVGAKLPSIRQLAA
                     SHQISRSPIMEAYDRLVSKGLIQPKHGSGFYVTGRVESHDHWFRGANPCVARAASGNI
                     LRQFNCAGDAPPLSSGFIPEAWRDVEGIAQAVRQISRKDTASLVDYAAPQGDATLREQ
                     ISRRLLQLEIETHPQHILVTHSASHALDLVARAMLRPGDTVFVEDPGYFNLFGLLKLQ
                     GIRLVGVPRARTGPDINVIEDLLAVHQPKLFFVNTVFHNPTATNIAPQVAFRLLQLAH
                     LHDFSIVEDDIYADLQAVPSQRLAALDQLNRVIYVGGFSKTLSSSLRLGYIAAHPELI
                     GNLMSVKTLTSMGGTKLAESIVATLLESGIYRKHMEKLRYRVDDALAHAVEQLQHSGW
                     EVFEKPCGGMFVWSRVPNIDDSAVLVAEAQKFGIAIAPGANYRPNNETSPWLRFNAAY
                     VRDNRTLVFLASASRL"
     gene            6593..6895
                     /locus_tag="VHV99_21110"
     CDS             6593..6895
                     /locus_tag="VHV99_21110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006028428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX3637933.1"
                     /translation="MFKVVIDPQGLEARLLPGSCLTELEFELYGQESVSFGCKVGACG
                     ACVVEVVEGLANLGIKGSDEQRFLETLGYSGEEFRLACQCHLAGAVRIRAVSSLHA"
     gene            6928..7680
                     /locus_tag="VHV99_21115"
     CDS             6928..7680
                     /locus_tag="VHV99_21115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006399254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637934.1"
                     /translation="MKNRVKNVGYENRIPFINAARSAELRAQIDSIIDGQIDQWYKTV
                     PYAAHLEGKTVDSAYYQRHLIETAWRIRLLRVAEAKALVEIAKRSPEAAQIWANYERE
                     EMLHDELFIQDLERAGVSREAFLATEPYLSTKLLTGYFSYLLDHEGPLGVVAYSYLVE
                     YVNVKLEPRKLEALKESVGERSIGGQISHSHTDINDDHPGEVWAAIRHLIRSDADFDE
                     LKRYLAEHQTVLAMYFKELYEDKIGSALEAAA"
     gene            7747..9048
                     /locus_tag="VHV99_21120"
     CDS             7747..9048
                     /locus_tag="VHV99_21120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006028426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX3637935.1"
                     /translation="MEDLVSACRARNLDVWILTSRPLPEHGAIRLAALQGMVDELFVS
                     NAHELSTHDVENAIASLRGRGFRIKGCISVWEGYRALMAYGNALQGVADLAWDRVEQL
                     RNKLHVRLALKDAGLTNVSAQTLTPAVLEALKHDRGVYFVKPVHGIASYGAFRLTQET
                     TWQVLDAVVSQAQRDTVYTSVLGTQLTFMAEEYISGTEFSFEVLAVAGTPFVVGIHEK
                     CQLTESDGTVLENSCTSPPSSLTNDQIAAGLSWIASVLTCLKLDWGCFHIEARFDGYR
                     WELIEVNPRVGGSLISRSVLALNGTASLLDLWLDQLIFQSDGKPSQDYMRAVAELSYS
                     KEGRAPTDQATFFRVYFASPGQIESIRVEPTEPRPVLTQVLLKEGDEIEPAAREVFLG
                     QILWHMPREQRDRDLKHLLASSATAIKVRYQTAEPQKDSRA"
     gene            9045..10391
                     /locus_tag="VHV99_21125"
     CDS             9045..10391
                     /locus_tag="VHV99_21125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006399251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase"
                     /protein_id="HEX3637936.1"
                     /translation="MSGQAPILLIVDYNLTRVSDVAKIARYARHQYDTQAMLIRAQPG
                     ARDLEICDYAIDLDPLAQDFVATALNLLAPFRERIRAGVVFSDNAVQRGAELLERLNL
                     PTDSAALAAGAFSKRVYREKEVRIRDLMEAQSIMVPHCASVVTVDDLRAFAEDHPAGF
                     VVKPSCEGNNRGVVIVRKGDSLETAFAIVKPYLANGAVCEQLIPFRREFSFDGIGETE
                     FITEKVNADGPYPVEVAQILPARLTVTERTTLTRAGRLANTIVGQREGPFHNEIKLSD
                     DGRQAAVVEPNRRPAGMKIWTLAQAVFGIDFYGLWVDAALGEKRHVAVVKTSTEAATV
                     MLGVPADGRYASPGHDKAEALLRKAISHAAAICGIAADVIDVLEYTWLATDARSIPVI
                     PQENSDFAAQVCIAIDGCRTDMRRLVQALRQSWIAVLGEEIERAAPRDDRYYDQVA"
     gene            10405..>11652
                     /locus_tag="VHV99_21130"
     CDS             10405..>11652
                     /locus_tag="VHV99_21130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015602995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate--amine ligase"
                     /protein_id="HEX3637937.1"
                     /translation="MKILILHRVPYGRIQYERGIDHDVNDVTYLGTKEALATLPSRLR
                     HRAVARPGKRSAFEEALDWRDLRHSRFERVISLSEYELLDAAKLRELLGIQGPTLQDV
                     QLVRDKVLMKHAVGTARIRIPRFARLRALLEKAETAQWTGQTVLKPHSGASSEDVVVF
                     ASLVDALRAVTTGSTGIPGLDEGTPALELYQVEEFISGDVLHFDGLVANGQVLTITAS
                     RYVGTCLGFASGEPLGSFHFPVSATTRQWVADVLAAVSTRNGSFHLEAIEAQAGLVFL
                     EVGNRVGGADVVATFELATGVHLPSEELRILIGGEPTRELPPTQSSGAWHGWFVFPGH
                     TSTESVYQGIEGIDTYRRDPGVVRWVELPEGHALQRKVTYSAGEAPLAGIVAFPDPAA
                     TQAWLRGLFSAARARSAYFNTTQD"
BASE COUNT         2336 a         3271 c         3713 g         2332 t
ORIGIN      
        1 tgaaggcgat cgagaatctg aagaagctgt cgcccgcgcc gtttggcgtg aaggacgacg
       61 cgcagtacat ccagcccgcc aattacgacg acgacatcga gaagctcgcc gagtgcgacc
      121 tcgtgatcga agcgatcgcc gaacgcatgg actggaagca cgacctgtac aagaaggttt
      181 cgccgcacat cgcaccgaat gcgatcttcg cgaccaacac ctcgggtctg tcgatcaccg
      241 atctgtcgaa cggtttcccg gatgaactga aggcgcgctt ctgcggcgtg cacttcttca
      301 atccgccgcg ctacatgcat ctggtcgaac tgatcccgac cgcgagcacg cgtccggaaa
      361 ttctcgatca actcgaatcg tttctgacga gcgtggtcgg caagggcgcc gtgcgggcga
      421 aagacacgcc gaacttcatc gccaaccgcg tcggtatttt ctcgatcctc gccgtgatta
      481 ccgaagccgc gaaattcggc ctgcgtttcg atgaagtgga cgacctgacg ggctcgcgtc
      541 tgggccgcgc gaagtcggca acgttccgca cggcggatgt agtcggtctc gatacgatgg
      601 cgcacgtcat caagacgatg caggacacgc tgaaggacga tccgttcttc ccggtctacg
      661 aaacgccggc tgtgctcgcc gaactggtga agaagggcgc gctcggtcag aagaccggcg
      721 gcggcttcta caggaaggaa ggcaaagcga tcaaggtgct cgacccgaag acgggcgggt
      781 atgtcgacgg tggcgcgaag gccgacgaac tggttggccg cattctgaag cgcccgccgg
      841 cggaacgtct gaagctcttg cgcgaatcgg agcatccgca agcgcaattc ctgtggtcga
      901 tcttccgcga cgtgtatcac tacatcggcg tgcatctcga atcgatcgcg gacaacgcgc
      961 gcgacgtcga tctggctatc cgttggggct tcggctggaa cgaaggtccg ttcgaaggct
     1021 ggcaaacggc cggctggaag caggtcgccg aatgggtgca ggaagacatc gcggcaggca
     1081 aggcgctctc gaatgtgccg ctgccgtcgt gggtgctgga aggtccggtt gccgagaagg
     1141 gcggcgtgca tacgaacgaa ggttcgtggt cgccggcttc gaagactttt gtgccgcgct
     1201 cgtcgctgtc cgtctacgac aagcaggtgt tccgtgcacc gctcgtcggc gaaacctcgg
     1261 ctgacccgaa gacctacggc aagacgctgt tcgaaaccga cgccgtgcgc gcctgggtcg
     1321 acgaccgtgc cggcgagaac gacgtcctga tcgtgtcgtt caagagcaag atgaacacga
     1381 tcggaccgtc ggtgatcgac ggtttgacgc aagccatcga actcgccgag aaggaataca
     1441 aggggctggt cgtgtggcag ccgacgtcgc tgaaactcgg cacgccgggc ggcccgttct
     1501 ccgcgggtgc gaacctggaa gaagccatgc ctgccttcat gatgggcggc gcgaagggta
     1561 tcgagccgtt cgtgcggaaa ttccagcaag gcatgctgcg cgtgaagtac gcgagcgtgc
     1621 cggtgatctc ggcggtgtcg ggcatcgcgc tcggcggcgg ctgtgaactg gcgctgcaca
     1681 gcgcgaagcg tgtcgcgcac atcgaaagct acatcggtct ggtcgaagtc ggcgtgggtc
     1741 tcgtgcccgc gggcggcggt ctgaaggaag cggcgttgcg cgcggcggag gcggcaacgc
     1801 aagtcggcgc gaccaacgat cttctgaagt tcgtgcagaa gtcgttcgaa aacgcggcga
     1861 tggcgaaagt ctcggcgtcc gcactcgacg cccgcgcaat gggctacctg aagccgtcgg
     1921 acacgatcgt gttcaacgtg ttcgaactgc tcgacgtcgc gaagaaggaa gcgcgtgcgt
     1981 tgtcggcagc gggctaccgc ccgccgctgc gtgtcacgca agtgccggtg gcgggccgct
     2041 cggcgattgc gaccatcaag gcatcgctcg tcaacatgcg cgacggccgg ttcattagcg
     2101 agcacgattt tctgatcgcc agccgcatcg cggaagcggt gtgcggcggt gacgtggaag
     2161 ccggcagtct ggtcgacgaa gaatggctgc tgaaactcga gcgtcgtgcg ttcgtcgatc
     2221 tgctcggcac gcaaaagacg caggaacgga tcatgggcat gctgcaaacc ggcaagccgg
     2281 tgcgtaatta acgcggacga ccgcacttcg tgcatgggac gagagtaagc gctaaagcgc
     2341 taactctcgc cgacagagga gcttcaaatg gcaaagcaat tgcaagacgc atacatcgtc
     2401 gccgcgagcc gcacgccgat cggcaaggcg ccacgcggca tgttcaagaa cacgcgcccg
     2461 gacgaactgc tggtgcatgc gatcaggtcg gccgtggcgc aagtgcccgg cctcgatacg
     2521 aaggtgatcg aagacgccat catcggctgc gcgattccgg aagcggaaca aggcctgaac
     2581 gtcgcgcgca tgggcgcgct gctggcaggc ctgccgaact cggtcggcgg cgtgacggtg
     2641 aaccgcttct gcgcatcagg cctcaccgcg ctggcgatgg cggcagaccg catccgcgtc
     2701 ggcgaatcgg acgcgatgat cgccggcggc tgcgaatcga tgagcatggt gccgatgatg
     2761 ggcaacaagc cgtcgatgtc gccgcacatc ttcgatcgca atgaagacat cggcatcgcc
     2821 tacggcatgg gtctgacggc agagaaggtc gcggatcgct ggaagatcag ccgcgaagcg
     2881 caagacgcgt tctcggtcga atcgcatcgc cgcgcgatcg ccgcgcagca agccggcgag
     2941 ttcaacgacg aaatcgccgc ctacacgatc accgagcgtt tccccgatct cgccaccggc
     3001 gaagtgaagg tgaagacgcg cgaagtctcg ctcgacgaag gtccacgtgg cgacacctcg
     3061 atggaaggcc tcgccaaact gcgcaccgtg ttcgccagca agggttcggt gaccgccggc
     3121 aacagttcgc aaacgtcgga cggcgcgggc gcgctgatcg tcgtgtcgga aaagatgctg
     3181 aaggaattca acctgacgcc gctcgcccgt ttcgtcagct tcgccgtgcg cggcgtgccg
     3241 ccggaaatca tgggcatcgg tccgaaggaa gcgattccgg ccgcgttgaa ggccgccggc
     3301 ctgaagatcg aagacatgga ctggatcgag ctgaacgaag cgttcgccgc gcaatcgctg
     3361 gcggtgattc aggacctcgg tctcgatccg tcgaagatca acccgcttgg cggcgcgatt
     3421 gcgcttggcc atccgctcgg tgcgacgggc gcgattcgtg cgtcgacggt ggtgcacggc
     3481 ctgcgccgcc gcaacctcaa gtacggcatg gtgacgatgt gcgtcggcac cggcatgggc
     3541 gcggcgggca tcatcgagcg tctgtgatcg tgggtgccct ggtccccgcg gtggtcgctg
     3601 gagcgatcct cgccgtgatt gccgcagtga cccccgcagt ggtcagctaa ggaccgcgga
     3661 aacggttcgc aggccgcctg gcttgcgaac cgttttttcc attcagcagc aaagaggagt
     3721 acgaggagat gacaatggat attctgctcg agcgcgccga cggcgtgctg acgattgcct
     3781 tcaaccggcc cgacaggaaa aacgcgatca cggccgcgat gtatcaggcc atggctgacg
     3841 cgctggtcga agcacagggc gacacgtcga tccgggtgat tctgattcgc ggcagtgccg
     3901 gcatcttcag cgccggcaac gatctcgaag atttcatgaa gaccccgccg atgggcgaaa
     3961 ccgcgccggt gttccagttc ctgcgtgcga tcagctccgc ggagcagccg gtggtggcgg
     4021 cggtggcggg gccggcggtc ggcatcggca cgacgctgct gctgcactgc gatctggttt
     4081 atgcggcgga tacggcgagc ttttcgctgc cgtttacgca actcgggctg tgtccggaag
     4141 cggcgtcgag tttgctgttg caacgcgtgg cgggctatca ggcggcagcg gaaaagctgc
     4201 tgctaggcga agccttcgat gcagccgaag cgcaccgcat gggcttcgtg aatcgtgtac
     4261 tgcccgccgc cgaagtcgat gcgttcgcgg cagcgcaagc ggcgaagctg gcggcgctgc
     4321 cggcctcgtc gttgcgcgtg acgaagagtc tgatgaagcg cgcgagccac caggaactgc
     4381 acacgcaaat gacagaggaa gcggtgcact tcggcaagat gctgctcgcg ccggaagcgc
     4441 gcgaggcgtt caaggcgttc ttcgagaagc gcaagccgga tttccgttcc ttcccgtagg
     4501 gatttatcgg cccttattgt ctcccggcgt gtcgtggcgc tcgcgccacg cgcgccgtcc
     4561 atcacccatc cagatttatc tacgacgccg ggagccgctc agttcggcga ttgacgtgcg
     4621 cctcttacag acgcgatgcg ctggcaagga aaaccaacgt ccgattgtct cgcacgtaag
     4681 cagcgttgaa ccgcaaccat ggagacgtct cgttgttcgg ccgataattc gcgccgggtg
     4741 caattgcgat gccgaatttt tgagcttcgg caaccagcac agcagagtcg tcgatgttcg
     4801 gcactctgga ccagacgaac atccccccgc acggtttctc gaagacttcc cagcccgaat
     4861 gctgcaattg ttcgactgcg tgcgccagcg cgtcgtccac ccggtaccgc agcttttcca
     4921 tgtgctttct gtaaatcccg ctttccagca gcgttgcaac gatgctttcg gcaagtttgg
     4981 tgccgcccat gctggttagc gtcttgacgc tcatgagatt gccaatcagt tcgggatgcg
     5041 ctgcgatgta tccgagccgg agggaagacg acagcgtctt cgagaaaccg ccgacataaa
     5101 tgacgcgatt cagttgatcc agcgcggcga gccgctgcga cggcactgcc tgcaaatcgg
     5161 catagatatc gtcctcgacg atcgaaaaat catgcagatg cgcgagttgt agcagccgga
     5221 atgcgacttg cggggcgatg ttagtcgccg tcggattgtg gaacaccgta ttgacaaaga
     5281 agagtttcgg ctggtgaacg gctagcagat cttcgatgac gttgatgtca gggccggtgc
     5341 gagcgcgcgg aacgcctacc agccggatgc cctggagctt gagtagtccg aagaggttga
     5401 aatagcccgg atcttcgacg aacacggtgt cgcctggcct gagcatcgct cgggcgacga
     5461 ggtcgagcgc atggctcgca ctgtgcgtga cgagaatatg ctgcggatgc gtttcgattt
     5521 ccagctggag gagtcgccgg gagatctgct cccgcagcgt ggcgtcgcct tgcggggcgg
     5581 catagtcaac cagactcgca gtgtccttgc gcgagatctg ccgcaccgct tgcgcgatgc
     5641 cttccacgtc acgccacgct tctgggatga atccgctcga cagcggggga gcatcgccag
     5701 cgcaattgaa ttgccgcagg atattgcccg acgccgctct ggcgacgcaa ggattggctc
     5761 ctctgaacca gtgatcgtga gattcgacgc gtccggtcac gtaaaacccg gagccgtgct
     5821 tcggctggat cagccctttt gaaaccagcc gatcgtaggc ctccatgatc ggggagcggc
     5881 tgatctgatg cgacgctgca agttgccgga tggaaggtag tttggcgcca acctgcactt
     5941 gacgtgagcg aatcatttct tctagttggc aggtcagctg ttccgcgagt gaaacaccct
     6001 ttgaagcgtc aagcaggatt ttcatggaat gcgttgtcca aaagtgttcg ccgcatcgca
     6061 atatacactt tctggtgaca cggttgaagt gttcattcca ttgcagtctg ttttgcgcaa
     6121 acatgtaact cgatcgccac tgcggtggcg caaataattt aataccaagt cggctgtgtt
     6181 ttttggaaag gcacaccttt aaaaatgtat cttattgatc tttatcgaga aattttcatg
     6241 ggctaagagg ctttgtttga ctggctatgc actcgcacgg ttgtgcatgc caatgctttt
     6301 ctcttgatat gcccgaataa atcttaaggc gatgaatgat tgcgttttct ccggtgggaa
     6361 aacgcaacga atcgttattg ctggccaagt gtttgtttgg ctccggaaac ttaaagatcg
     6421 ccaacatagg ttcacgtgtc ttatttttca gatagcgaat ttaaaaaaat gtggcgcggc
     6481 cattaagttt ccgttccggg taatttgtat atttttgttg cagtggcttt tgggtccaat
     6541 ccggatttcg gtgaagataa aacaggggtt gatatgacgg ggcggagggc gtgtgtttaa
     6601 agtcgtaatc gacccgcaag gccttgaggc acggctgttg cctggcagtt gccttaccga
     6661 actcgagttc gaactgtacg gccaggagtc ggtttcattt ggctgcaagg tgggagcctg
     6721 cggcgcttgc gtggttgagg tcgtcgaagg actggcgaac ctgggcatca aggggagcga
     6781 tgagcagcgc ttccttgaga cgctcggcta ttcgggggag gagttccggc tcgcctgtca
     6841 atgtcacctg gccggcgcag tgagaatccg ggcagtgtcc tcgctgcacg catgaacatt
     6901 caatggattc aactgggagg aaaaatcatg aagaatcggg tcaagaatgt cggttatgaa
     6961 aatcggattc cgttcatcaa cgcggctcgc tctgcggagt tgcgcgcgca gatcgactca
     7021 atcatcgatg gtcaaatcga tcagtggtac aaaaccgtgc catacgcggc ccatctggaa
     7081 ggcaagacgg tcgactcggc ttactatcag cgccacctga tcgagacggc gtggcgtatc
     7141 cgcctgttgc gcgttgccga agcgaaggcg cttgtggaaa tcgcaaagcg cagccccgag
     7201 gcagcccaga tctgggcgaa ctacgagcgc gaggaaatgc tccatgacga gctgttcatt
     7261 caggatctag aacgagccgg cgtgtcccgg gaagcattcc tcgcaacgga gccgtatctg
     7321 tcaacgaaat tgctgacggg ttacttctcc tatctcctcg accacgaagg gccactcggc
     7381 gtcgtcgcct attcctatct ggtggaatac gtcaatgtga agctggagcc tcgcaagctg
     7441 gaggcgctca aggaatcggt cggtgagcgg agcattggtg gacagatctc gcactcgcac
     7501 accgacatca acgacgatca tcccggcgaa gtctgggctg ccattcgtca cctgattcgc
     7561 agcgacgcgg acttcgacga gttgaagcgc tacttggccg aacatcagac ggtgttggcg
     7621 atgtacttca aagaactcta cgaagacaag atcggctcgg cgctcgaagc tgcggcctga
     7681 cgctaacggg gagacacaat gaagtgcgat ggcgttctca tccttagtca ctgcggattc
     7741 tcgttcgtgg aagatcttgt ttcagcctgc cgcgcgagaa atctggacgt gtggatattg
     7801 acttccaggc cactaccgga gcacggtgcg attaggcttg cggctctgca gggaatggtc
     7861 gatgaacttt ttgtgagtaa tgctcatgaa ttgagcacgc acgacgtcga gaacgctatc
     7921 gcttcgttgc gtggccgcgg ctttcgtatc aagggatgta tcagcgtctg ggaaggctat
     7981 cgtgcgctga tggcatacgg caacgcgcta cagggcgttg ctgaccttgc gtgggatcga
     8041 gtggagcaac tgcgcaacaa gcttcacgtg cgtcttgcgc tgaaggacgc cggactcacg
     8101 aacgtgagtg ctcaaacgct tacgccggcg gtgctcgagg cgcttaaaca cgaccgtggg
     8161 gtgtacttcg tcaagcccgt tcacggcatt gcctcttatg gcgccttccg gctgactcag
     8221 gagacgacct ggcaggttct cgacgcagtt gttagtcagg ctcaaaggga cacggtatac
     8281 acctctgttc tcggaacaca gctcacgttc atggcggaag aatacatctc cggcacggag
     8341 ttcagtttcg aggtcctggc ggtggccggc acgccgttcg tcgtaggtat tcatgaaaaa
     8401 tgtcaactaa cggaaagtga cgggaccgtg ctggagaaca gctgtacaag cccaccttca
     8461 tcgctaacga acgatcagat agccgcgggg ttgtcctgga tcgcctcggt gctgacctgc
     8521 ctcaagctgg actgggggtg ctttcacatc gaagcacggt tcgatgggta ccgctgggag
     8581 ttgatcgagg tcaatccgag agtcggtggc agccttattt cgcgcagtgt cctggcgctc
     8641 aacgggactg ccagcctgct cgatttgtgg ctcgatcaat tgatttttca gtcggacggc
     8701 aagccatcgc aagattacat gcgcgctgtc gctgagttgt cgtactcgaa agagggacgg
     8761 gcgcccaccg accaggcgac ctttttccgg gtctattttg cgtcaccagg gcaaatcgaa
     8821 agtatccggg tcgaacctac tgaaccgcgt cccgtactca cgcaggtgct gttgaaggag
     8881 ggagacgaaa ttgaaccggc ggcgcgggag gtctttctcg gacagattct ctggcacatg
     8941 ccgcgtgagc aacgcgaccg cgatctgaag cacctgctgg cgtcgtccgc gacggccatc
     9001 aaggtgcgct atcagaccgc tgagccccaa aaggacagta gagcatgagc ggacaagccc
     9061 caatccttct gatcgtcgat tacaacctga cgcgcgtttc cgacgtcgca aagatcgccc
     9121 gatacgcaag acaccaatac gatacccagg cgatgctgat ccgcgcacag cccggggctc
     9181 gtgatctgga gatttgcgat tacgcgattg acctggatcc gctggcacaa gattttgtcg
     9241 cgacagccct gaaccttctt gctccgtttc gggagcgcat tcgcgcgggc gtagtcttct
     9301 ccgacaacgc ggttcagcga ggcgcggagt tgctcgagcg tctgaacttg ccgacggact
     9361 ccgccgcact cgctgcaggg gcgttcagca agcgcgtgta tcgcgagaag gaagtccgga
     9421 ttcgagatct catggaggct cagtcgatca tggttccgca ctgtgcaagt gtggtcactg
     9481 tcgacgatct tcgggcgttc gctgaagacc atccggcggg ttttgtcgtg aagccgtcgt
     9541 gcgaaggaaa taacaggggc gttgtcattg tgcgtaaggg agatagcctg gagactgcct
     9601 ttgcgatcgt caaaccttac ctggcgaacg gagcggtttg cgagcagctg attccttttc
     9661 gccgcgagtt ttcgttcgat ggaatcggtg aaacggaatt cattacggag aaggtcaatg
     9721 ccgatggccc gtacccggta gaggttgcgc aaatccttcc cgcgcgcctc accgtgacgg
     9781 agcggactac gctgacccgc gccggccggc tggccaatac gattgtcggc cagcgcgagg
     9841 gtccattcca taacgagatc aagctgagcg acgatggccg tcaggcggct gtggttgagc
     9901 cgaatcgcag gcccgccggc atgaaaatct ggacgctcgc gcaagcggtc tttggcatcg
     9961 atttctatgg cctctgggtt gacgcggcgt tgggtgaaaa acgtcacgtt gctgtcgtca
    10021 agacctctac cgaggcggct acggtgatgc ttggcgtacc ggcggacggc cgctatgcgt
    10081 ctcccgggca cgacaaggcc gaagcgttac tacgtaaggc catttctcat gcggcggcga
    10141 tctgcggcat tgcggccgac gtgatcgacg tgctggagta cacgtggctg gctactgatg
    10201 cgcgctcgat tccggtcatc ccgcaagaga attccgattt cgccgcacag gtttgcattg
    10261 cgatcgacgg atgccgcacc gatatgcgcc gcctcgtaca ggcactgcgg caatcgtgga
    10321 tagcggtgct gggtgaagag attgaacgcg cggcgcctcg cgacgatcgc tactacgatc
    10381 aggtcgcctg aagggaatgt ccagatgaaa atcctgatcc ttcatcgcgt tccctatgga
    10441 cgcattcagt acgagcgtgg catcgatcac gacgtcaatg acgtcaccta tctgggtacg
    10501 aaggaagctt tggccacgtt gccgtctcgc ctgcgccatc gggcggtggc ccgcccgggc
    10561 aagcgcagcg catttgaaga ggcgctggac tggcgtgacc tgcggcatag ccggtttgag
    10621 cgggtcattt cgctttcgga gtatgagttg ctcgacgcgg cgaaactgcg cgaattgctg
    10681 gggatacaag gacccacgct acaagacgtg cagttggtgc gtgacaaggt cttgatgaag
    10741 catgccgtcg gaaccgcgag gatccgcatc ccacgctttg cccgcttgcg cgcactgctt
    10801 gaaaaagcag agacggcgca gtggaccggg cagacagtgc tgaagcctca tagtggcgca
    10861 tccagcgagg acgttgtggt ctttgcgtcg ctggtcgatg ccctgagggc ggtgaccacc
    10921 gggtcaaccg gcattccggg actagatgag ggtacgccag cgcttgagct gtatcaagtg
    10981 gaggaattca tctcaggaga cgtcttgcat ttcgacgggc tcgtcgcaaa cggtcaagta
    11041 ttgaccatca cggccagtcg ctatgtcggg acgtgtctcg ggtttgcgag cggcgagcca
    11101 ctcgggtcgt tccacttccc tgtgtccgcg actacacgcc aatgggttgc cgatgtgctg
    11161 gccgcggtat ctacccgaaa cggcagcttc catctggaag cgattgaagc acaggcgggt
    11221 cttgtatttc tcgaggtggg caaccgggtc ggaggggctg atgtggtggc gaccttcgaa
    11281 ctggcaacag gcgtgcatct gccatcggaa gagttgcgca tcctgatcgg cggcgagccg
    11341 acgcgggaat tgccgccgac gcaatcttcc ggtgcgtggc atggctggtt tgttttcccc
    11401 ggacatacga gcaccgaatc cgtctatcaa ggtatcgagg gtattgacac gtaccgacgg
    11461 gatccgggcg tcgttcgatg ggtggaattg ccggaaggcc atgccttgca acgcaaggtg
    11521 acctattcgg cgggtgaggc gccgctggcg ggcattgtcg cttttcccga cccagcggcc
    11581 acgcaagcgt ggctgcgagg attgttttcc gccgctcgcg cacgctcagc gtatttcaac
    11641 acaacgcagg ac
//
LOCUS       DATASH010000788         3608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5272614,
            whole genome shotgun sequence.
ACCESSION   DATASH010000788 DATASH010000000
VERSION     DATASH010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5272614"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VHV99_21135"
     CDS             complement(<1..651)
                     /locus_tag="VHV99_21135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016354439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX3637938.1"
                     /translation="MKKTIVATAVLGSFTLTAHAQSSVTLYGLIDAGISYVSNARAGT
                     GHDSLVKYDDGVASGSRWGLRGNEDLGGGLKALFVLENGFNSGNGTLGQGGALFGRQA
                     YVGLSKDTIGSLTFGRQYSFSTDYLGGSYSTGSQTAAGNYAYHINDVDQLVSSRINNA
                     VKFNSVNFSGFTFGALYGFSNQAGAFAGAPATGTTAAPVAGSSRAYSFGVNFANGPF"
     gene            1563..1982
                     /locus_tag="VHV99_21140"
     CDS             1563..1982
                     /locus_tag="VHV99_21140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1810 domain-containing protein"
                     /protein_id="HEX3637939.1"
                     /translation="MDDLYDLQRFVDAQDPVYAQACDELRNGRKRSHWMWFVFPQIQG
                     LGSSAMAQRFAISSLAEAEAYLRHPLLGPRLRESTRLVNLVEERSIEAIFGYPDYLKF
                     RSSISLFARATSDNEVFVEALRKYFGGEADPQTLHLL"
     gene            complement(2054..3097)
                     /locus_tag="VHV99_21145"
     CDS             complement(2054..3097)
                     /locus_tag="VHV99_21145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013209204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase"
                     /protein_id="HEX3637940.1"
                     /translation="MLAIARKRVVATVNKILEAGPAPWFASALASAALTIGSAQAQPP
                     SFETFAVPTGSAPHDVAPAPDGAVWYTAQAQGAVGRLDPRSGRIDKIPLGAGSAPHGV
                     IVGPDRAAWVTDGGQNAILRIDPKTLAVTRFPLPKDRPNANLNTAVFDKQGHLWFTGQ
                     SGIYGELDPQHARMRVFDAPRGPGPYGICVTPDGSLYFASLAGSYLGRIDPGSGAAQV
                     IEPPTSNQGARRAWSDSKGRVWISEWNAGKVGVFDPATHQWREWRLPGNDPHAYAIFV
                     DNQDIVWLSEWSANALVRFDPRAERFDVFLLPRPHANVREMMGRPSEVWLSESGTDHL
                     LLYRSRAANASGN"
BASE COUNT          699 a         1062 c         1111 g          736 t
ORIGIN      
        1 gaacggaccg ttcgcaaaat tcacgccgaa actgtatgca cgcgacgatc ctgcgaccgg
       61 tgccgcggtc gtaccggtgg ccggtgcgcc ggcaaatgcg cctgcctgat tcgagaagcc
      121 gtacaacgcg ccgaacgtga agcccgagaa attcacgctg ttgaacttca ctgcgttgtt
      181 aatgcggctc gacaccagct ggtcgacgtc gttgatgtgg taggcgtaat tgcctgcggc
      241 agtctgactg ccagtcgagt aactgccgcc gaggtagtcg gtcgagaacg aatactgccg
      301 accgaaggtc agcgagccga tggtatcctt cgaaagaccg acatacgcct gacgaccgaa
      361 cagggcaccg ccctgaccga gcgtaccgtt gccgctgtta aaaccatttt cgagaacgaa
      421 aagggctttc aggccgccgc cgaggtcttc gttgccgcgc aggccccaac ggctgcccga
      481 tgcgacgcca tcgtcgtact taacgaggct gtcatggccc gtgccggcgc gtgcattgct
      541 aacgtagctg atgccggcgt cgatcaatcc gtaaagcgtc acgctacttt gcgcatgagc
      601 ggtcagcgtg aagctcccaa ggactgccgt tgccacgatg gtctttttca tttattccct
      661 tctttttatg gtgatggtac gacgcaagcc agcaagcgtc gcgtgagccg ggattgcgaa
      721 gctgcagtag tgcgtcggtc aaccttcctg cgctagttac gtaaattaga agtcttcgcg
      781 ccactcgtcc gtctgcttcg cccggcgacc cagccgagca gggctattgg cagcgaccct
      841 atgctagggt cattgctgta ttgcgaatag ttaaagttta ttattgtgtt gattcacatt
      901 cgcttatata tcgaccatga ggacgggtaa cgcgacactc acgtaattca tgtcgcggcc
      961 aaattttgag tggccggatc tccgggaacc cgatgtaact gccagtgccg tgcgggatga
     1021 ttgctgcaac agtcgaatgg agcgcgttac attgccgcgt gcgcggcatc aaccgaaccg
     1081 ccgactgagc ctcgttggct cgggttatgc ggtggatcgt caagctcggc gacgatatgc
     1141 attccaggca cgaccgctgg gctgggacca accgtcacgt ggcgcggctt tcgtactctc
     1201 cacgaactgg accgaaatga gtcgtgaacc gcctcggatc tttaggcaga acgagtgatg
     1261 tgttcagctt tagatttggg gaaacgttag cacatcaatt cggaggacta ctcacgttca
     1321 taaatcagac ggtcgccgat cctggtcgac cgtctccagc gggtcgacct cagccgatcg
     1381 caccgcggag gcgatgtcgc gggagcgcga aggccgccga tgcgctatcg ttaaaaacgc
     1441 aactctcgat gccgcattgg gcagcaggta attgccgcgg cgggcagcgg acgaggtggg
     1501 aaacaggtgc gagaatcggg gttcaagggt ggatttccac accggcatca acggaatacg
     1561 aaatggacga cctatacgac cttcagcgct ttgtcgacgc tcaagaccca gtgtatgcac
     1621 aggcatgcga tgaattgagg aatggacgta agcgaagcca ctggatgtgg ttcgtgtttc
     1681 cgcaaatcca gggccttggg tcaagcgcga tggcgcagcg gttcgctatc tcgtcgttgg
     1741 ccgaggctga agcctatctg cgccacccgt tactcggccc acggctgcgc gaatcgacgc
     1801 gactggtcaa cctcgtcgaa gagcgttcca tcgaggcaat tttcggatat ccggattacc
     1861 tgaagtttcg atcgtcgatc agcctgttcg cgcgcgccac gagcgacaac gaggtatttg
     1921 tggaagctct gcgcaagtat ttcggcggtg aagccgatcc gcaaacgttg cacttactct
     1981 aggccgtttt tgcgctgcgc ctgcgcgcgc ggcggagcgg acttcggaat ttcagtgtcg
     2041 gtactgtggg gcgtcaattc ccgctcgcat tggctgcgcg ggaccggtag agcagcagat
     2101 gatcggtgcc ggattcggac aaccagactt cgctcgggcg gcccatcatc tcacgtacat
     2161 tcgcgtgcgg gcgcggcagc agaaacacgt cgaaccgctc ggcacgcggg tcaaagcgca
     2221 ctagcgcgtt ggcgctccat tcactgagcc agacgatatc ctggttgtct acaaaaatgg
     2281 cgtatgcgtg cggatcgttg ccgggcaaac gccattcgcg ccactgatgc gtcgcgggat
     2341 caaatacgcc gacttttcct gcgttccact cgctgatcca gactcgcccc ttcgaatcgg
     2401 accatgcgcg gcgcgcgccc tgattcgacg ttggcggctc gatgacctgc gcagcaccac
     2461 tcccgggatc gatgcggccg agataactcc ctgccagcga cgcgaaatac aggctaccgt
     2521 ccggggtcac gcagatgccg tagggcccgg gcccgcgcgg cgcatcgaag actcgcatgc
     2581 gggcgtgctg cggatccagt tcgccataga tgccgctttg ccccgtaaac cagagatgcc
     2641 cttgcttgtc gaatacggcg gtgttcagat tggcgttcgg ccggtccttc ggcagcggaa
     2701 aacgcgtgac ggcgagcgtc ttcggatcga tgcgcaaaat cgcgttctgg ccgccgtcag
     2761 tcacccacgc cgcgcgatcg ggacccacga tcacgccgtg cggcgccgat ccggcgccga
     2821 gcggaatctt gtcgatcctg ccgctacgcg ggtccagccg tccgaccgcg ccctgcgcct
     2881 gcgcggtgta ccacacggcg ccgtccggcg ccggtgcgac gtcatgcggc gcactgccgg
     2941 tcgggacggc gaaggtctcg aacgacggcg gctgcgcctg cgccgagccg atggtgagcg
     3001 cagcgctcgc caaagccgac gcaaaccacg gtgccggccc cgcttcgagg atcttgttca
     3061 ccgttgccac aacccgtttc ctcgcgatcg ccaacatgtt cgcctccgtg tcacgggatg
     3121 agtttcaaag ggcgctgaca ggtttttatt gttgcactta tctgtagccg tcgctgcgtc
     3181 gactcattcg ccacgccggc cccggtggca atttaagttc acggtggcga agatcaatat
     3241 cgaagggagg atcatgtcga tggccgtggc tcaaatcgcg gccgaacccg gttgtccaag
     3301 cccgtgtgga tgggttccca tttctcgagt gggaccttaa cgaaggggtg gccgagaggc
     3361 ggctgcgact gtcaatcatg aggtcgtgtt cggccagtct cagccgttcg aagaagattt
     3421 gcgaccgttc gttggtagtc tgacaggagt cactcacggg cgaggccacc agatcgtcac
     3481 ccgtgcaacg gcgatgtcgt tgaagtagac gcgacactcg ctcgttgcgc aggcggcaaa
     3541 gcggtgagcg gaccgttatg cggtcgtgtt gatgttgctg cccagtgcta cgccattgag
     3601 tgtgtagg
//
LOCUS       DATASH010000789         3822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2703101,
            whole genome shotgun sequence.
ACCESSION   DATASH010000789 DATASH010000000
VERSION     DATASH010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2703101"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..71
                     /gene="rpsI"
                     /locus_tag="VHV99_21150"
     CDS             <1..71
                     /gene="rpsI"
                     /locus_tag="VHV99_21150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX3637941.1"
                     /translation="REVERKKVGFHKARRLKQFSKR"
     gene            338..703
                     /gene="erpA"
                     /locus_tag="VHV99_21155"
     CDS             338..703
                     /gene="erpA"
                     /locus_tag="VHV99_21155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007587442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEX3637942.1"
                     /translation="MNAVTDTPVTEMPAPFVFTDAAADKVKQLIEEEGNADLKLRVFV
                     QGGGCSGFQYGFTFDEAVNEDDTVMNKSGVQLLIDSMSYQYLVGAEIDYKDDINGAQF
                     VIKNPNATTTCGCGSSFSV"
     gene            complement(790..1920)
                     /locus_tag="VHV99_21160"
     CDS             complement(790..1920)
                     /locus_tag="VHV99_21160"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEX3637943.1"
                     /translation="MAQDPADGVYFGLMSGTSMDGVDGVAVRFAEGKPPQVLAEAFVG
                     FAAGLREALFALQQSGDNEIEREALAANALATRYTVCCHELLQASRVPASEVRAIGVH
                     GQTVRHRPERGYTRQINNPALLAEMMHIDVIADFRSRDVAAGGQGAPLVPAFHATVFG
                     AKDETRVVCNLGGISNITILNATGSVRGFDCGPANALLDEWAQRHLGKPFDENGHFAA
                     GGQVDRPLLNALLDEPFFSQQPPKSTGRDLFNAEWLDAKLQPFAAVAPADVQATLVAL
                     TAVTVAREIERHASDAKAVYVCGGGARNPEILKALQQALEDSGVSGVPVMTTDALGVP
                     PSQVEPLAFAWLAMRCMARLPGNLPAVTGAAAERVLGAIYPR"
     gene            2176..3420
                     /gene="tyrS"
                     /locus_tag="VHV99_21165"
     CDS             2176..3420
                     /gene="tyrS"
                     /locus_tag="VHV99_21165"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX3637944.1"
                     /translation="MTTESTKPSPAFPITDEVRHALAVTKRGVDELLIEDEFAQKLAK
                     SQATAKPLRIKLGLDPTAPDIHIGHTVVLNKMRQLQDLGHTVIFLIGDFTSLIGDPSG
                     RNATRPPLTREQIESNAKTYFEQAALVLDRDKTEIRYNSEWSMPLGADGMIKLASRYT
                     VARILEREDFTKRFQGGVPISIHEFLYPLMQGYDSVALNADLELGGTDQKFNLLVGRE
                     LQKQYGQEQQCILTMPLLEGLDGVEKMSKSKNNYIGISEKPTDMFGKLMSISDVLMWR
                     YFELLSFRPMDEIAGFRKEIEAGRNPRDFKVMLGQEIVARFHSQADAERALEDFNHRA
                     KGGVPDDIPSVTLAGAPLAIGQLLKQANLVPSTSEALRNIEQGGVKIDGTTVSDKGLK
                     VEAGEYVVQVGKRRFARVTLTA"
     gene            3461..>3822
                     /gene="dtd"
                     /locus_tag="VHV99_21170"
     CDS             3461..>3822
                     /gene="dtd"
                     /locus_tag="VHV99_21170"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEX3637945.1"
                     /translation="MIALIQRVRRAEVRVGERVTGAIEAGLLALVCAERGDTDAAADR
                     LLAKVLGYRVFSDAAGKMNLSVQNLDGAGRAGGLLLVSQFTLAADTNSGLRPSFTPAA
                     PPDEGERLFDYFVTAARA"
BASE COUNT          736 a         1208 c         1171 g          707 t
ORIGIN      
        1 ctcgtgaagt cgaacgtaag aaggtcggtt tccacaaggc acgtcgcctg aaacaattct
       61 cgaagcgtta atcgtttcgg gtgcgagtcg gctctcgggc cgatccgcag caaaagccgc
      121 caacgctttc gcgaaggcgg ctttttttat ggatttgcgg cttggcgtcg cgctaacggt
      181 tttattgccg attggcgatt tgatccgctc aggcaagctg gcggcggcat gagggccgtc
      241 cactgaatta ccataagaac ccattgattt tgtgggcttc ggcacgatat cgagatcagc
      301 cctacaatag cggttagaag ctttttggag agttcgaatg aacgcagtca ccgacacacc
      361 cgtgaccgag atgcccgccc ccttcgtttt taccgacgca gcggctgaca aggtcaagca
      421 actgatcgaa gaggaaggca atgcggacct caaactgcgc gttttcgtgc agggcggcgg
      481 ctgctcgggc tttcaatacg gtttcacgtt cgacgaagcc gtcaacgaag acgacaccgt
      541 catgaacaag agcggcgtcc agttgctgat cgattcgatg agctatcagt atctggttgg
      601 cgccgaaatc gactataaag acgacatcaa cggcgcgcag ttcgttatca agaatccgaa
      661 cgcgaccacc acgtgtggtt gcggctcgtc gttctcggtt tgatggcaaa ccgttccggt
      721 gacaggtggt agagaaaacg gggcttcggc cccgtttttt attgcctgag ctaaacctgg
      781 gctcctcgat tagcgcgggt agatcgcccc cagcacgcgc tcggcagcgg cgccggtgac
      841 tgccggcaaa ttgcccggca gccgcgccat gcagcgcatt gccagccatg cgaacgccag
      901 cggctcaacc tggcttggcg gcacgccgag cgcgtcggtt gtcatcacgg gtacgccact
      961 gacgccgctg tcctccagcg cctgttgcaa cgctttaaga atctccggat tgcgcgcccc
     1021 cccgccgcac acataaaccg ccttggcatc cgatgcatgc cgttcgatct cacgcgccac
     1081 cgtcaccgcg gtcagcgcga ccagcgtcgc ttggacgtcc gcgggggcaa ccgcggcaaa
     1141 cggctggagt ttcgcatcga gccattcggc gttgaacagg tcgcggcctg tgcttttcgg
     1201 tggctgttgc gagaagaagg gctcgtcgag caaggcgttc agcagcggac gatccacctg
     1261 gccgccggcc gcgaagtggc cattttcgtc aaaaggcttg ccgagatggc gctgcgccca
     1321 ctcgtcgagc agcgcgttcg ccggcccgca gtcgaagccg cgcacgctgc cggtcgcgtt
     1381 caggatcgtg atattgctga tgccgcccag attgcagacc acccgcgtct cgtctttcgc
     1441 accgaatacg gtggcgtgaa acgccggcac aagcggcgcg ccctggccgc cggccgcgac
     1501 atcgcggcta cgaaaatccg cgattacgtc gatatgcatc atttccgcga gcagcgccgg
     1561 gttgttgatc tgccgcgtgt aacccctttc cggccgatgc cgcaccgtct gaccgtggac
     1621 gccgattgcg cgcacttccg aggccggcac gcggctggcc tgcagcaatt cgtggcagca
     1681 cactgtgtag cgcgtggcga gcgcattggc agccagagct tcgcgttcga tctcgttgtc
     1741 acccgattgt tgcagcgcga acaacgcctc gcgcaagccc gctgcgaagc cgacaaacgc
     1801 ctcggccagc acctgcggcg gtttgccttc ggcgaatctg acggccacac cgtccacgcc
     1861 gtccatgctt gtgccggaca tcagtccaaa gtagacgcca tctgcggggt cttgcgccac
     1921 gttgcagctc ctctcgaggg ttcgttgcga ttcgttgtag cagattatcg gggcaagcgc
     1981 ggccgaattt aaagcgtcgc tatgctggcg gcgcgacgcc gagcgtggaa atcccacgcc
     2041 agataagccg tttgcggccc ttacggacgc tcatgaccac ttgaagtcat taagatcgcg
     2101 tgagcctgcg tctatgggcg cggcggcgcg catctatggg acaatctcct tttttcgcga
     2161 cttcacgttt ccagcatgac caccgagtcc accaagccca gccccgcctt cccgatcacc
     2221 gacgaagtcc gtcacgcgct cgcggtcacc aaacgcggtg tcgacgaact gctgatcgaa
     2281 gacgaattcg cgcaaaagct cgcgaagagc caggcgacgg ccaagccgct gcgcatcaag
     2341 ctcggcctcg atccgaccgc gcctgatatc cacatcggcc atacggtcgt gctgaacaag
     2401 atgcgccagt tgcaggatct cggtcacacg gtgattttcc tgatcggcga cttcacgtcg
     2461 ctgattggcg atccctcggg ccgtaacgcc acgcgtccgc cgcttacgcg cgagcagatc
     2521 gaatcgaacg cgaagacgta cttcgagcag gccgcgctcg tgctcgaccg cgacaagacc
     2581 gaaatccgct acaacagcga atggtcaatg ccgctcggcg ccgacggcat gatcaagctc
     2641 gcatcgcgtt acacggtggc gcgtattctc gagcgcgaag atttcaccaa gcggttccag
     2701 ggcggcgtgc cgatctcgat ccacgaattc ctgtacccgc tgatgcaagg ctacgactcg
     2761 gtcgcgctga atgccgacct cgagctcggc ggcacggacc agaaattcaa cctgctggtg
     2821 ggtcgcgaac tgcagaagca atacggtcag gaacagcagt gcatcctgac catgccgctg
     2881 ctcgaaggcc tggacggcgt cgagaagatg tcgaagtcga agaacaacta catcggtatc
     2941 agcgaaaagc cgacggacat gttcggcaaa ctgatgagta tttccgacgt gctgatgtgg
     3001 cgttacttcg aactgctgtc gttccgtccg atggacgaga tcgccggttt ccggaaagag
     3061 atcgaagcgg gccgcaaccc gcgcgatttc aaggtgatgc tcggtcagga aatcgtcgcg
     3121 cgcttccact cgcaagccga cgccgagcgc gcgctcgaag acttcaacca tcgcgcgaag
     3181 ggcggcgtgc cggacgatat cccgtccgtg acgctcgcgg gcgcaccgct cgcaatcggc
     3241 cagctgctca agcaggccaa cctcgtgccg tccaccagcg aggcgctgcg caacatcgag
     3301 cagggcggcg tgaagatcga cggcacgacc gtgtccgaca agggccttaa ggtcgaagca
     3361 ggcgagtacg tcgtgcaggt cggcaagcgc cgttttgcgc gtgtgacgct gacggcgtga
     3421 tgggacgcgg catgcctcgt cttaagctgg actgctgcgc atgatcgcgc taatccagcg
     3481 cgtgaggcgc gctgaagtgc gcgtcggcga acgtgtcacc ggcgccatcg aagcgggact
     3541 gctcgcgctg gtgtgcgccg agcgcggcga taccgacgca gccgccgacc ggctgctggc
     3601 gaaggtgctt ggctaccgcg tgtttagcga cgcggctggc aagatgaacc tctccgtgca
     3661 gaacctcgac ggggcaggac gcgccggcgg cctgctgctg gtctcgcagt tcacgctggc
     3721 cgccgatacc aacagcggcc tgcgcccgag cttcacaccg gccgcgccgc ccgacgaggg
     3781 cgagcgcctg ttcgattact tcgttacggc cgctcgcgcc ag
//
LOCUS       DATASH010000790         4039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3097642,
            whole genome shotgun sequence.
ACCESSION   DATASH010000790 DATASH010000000
VERSION     DATASH010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3097642"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="VHV99_21175"
     CDS             <1..421
                     /locus_tag="VHV99_21175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEX3637946.1"
                     /translation="ERVVQLNVQTVKTSLSEQQALGYAALSAQSVSEVIDLQSQQLPA
                     AVTKTFAYWRHVADIAVQTRNELFSAMQEHFGSSLQTFAKIFDVASSGGFVAQERIAR
                     SSLLVMTEPAVAAAERVAIVDSSGKVVSSDDVRGDLH"
     gene            708..1235
                     /locus_tag="VHV99_21180"
     CDS             708..1235
                     /locus_tag="VHV99_21180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3637947.1"
                     /translation="MTPNFESQECPNDSTGSQRGRFQNGAPVHVLVVDDSRPSADALG
                     AYLQAGGMSVRTVYHGSAALREAKAWIPDRIVLDVAMPGLSGIGVAAAQRSIEATGKI
                     PLLAFTAFDTADYIREMKEVGFDAVCRKPSELDQLEGMILKLLGQNAVDGFQIVPSTA
                     EPGPSDPGLDTVDRH"
     gene            complement(1460..2395)
                     /locus_tag="VHV99_21185"
     CDS             complement(1460..2395)
                     /locus_tag="VHV99_21185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637948.1"
                     /translation="MNHTRDPADYPLAAKDPALLVRQHEAAVSWGAVFAGGIGAAAFA
                     LILLTLGTGLGLTSISPWSSGPSNAKAFGFAAVVWICVTSILTSGLGGYLAGRLRSRW
                     LAVDADEIHFRDTAHGFLSWAVATLFTAAILTSAVSGIVRTGAQTAATTAAAGGSVAM
                     GTMQRGNAEAATNTWPLGYFVDSLFRNPSGSSLAAPATPEGRNDTARAETARIFLNSA
                     ATGDHLSPEDTAYLSQLVAQHTGLTPEAAQARVVTTYTRLLQKITALDAAAKDAADKA
                     RKVTVGASLWLFISLLMGAFSASLMATHGGRVRQL"
     gene            2626..3574
                     /locus_tag="VHV99_21190"
                     /pseudo
     CDS             2626..3574
                     /locus_tag="VHV99_21190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591820.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
     gene            complement(3505..3663)
                     /locus_tag="VHV99_21195"
     CDS             complement(3505..3663)
                     /locus_tag="VHV99_21195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637949.1"
                     /translation="MARCKRAPEGVTLVELKDALPEVGGGKLEAFRPNSSTQSAGCPS
                     RPARPVSW"
     gene            3730..>4039
                     /locus_tag="VHV99_21200"
     CDS             3730..>4039
                     /locus_tag="VHV99_21200"
                     /inference="COORDINATES: protein motif:HMM:NF027450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family zinc-binding domain-containing
                     protein"
                     /protein_id="HEX3637950.1"
                     /translation="MRRSRARNDTQDSVHDAVANAAAQFEAMSVHDKETLQRMIDYAQ
                     TGECRWRAILAHYGDTPSMQRCGVCDNCMNPPYIALAEQSIAVDDTLRDADASKPSSR
                     P"
BASE COUNT          807 a         1203 c         1279 g          750 t
ORIGIN      
        1 tgagagggtt gtccaattga acgtccagac tgtcaaaacc tcactgtccg agcagcaggc
       61 gttgggctac gcggcgcttt ctgcccaatc cgtcagcgaa gtaatcgatc ttcagtcaca
      121 gcagttgcca gccgcggtca cgaagacctt tgcatactgg cggcatgtgg cagacatcgc
      181 tgttcaaacg cgaaatgagc tcttctcggc gatgcaggag cacttcggaa gttcgcttca
      241 gacgttcgca aagatattcg acgtcgcgtc gtccggcgga ttcgtcgctc aggagcggat
      301 cgccagatcg agtctgcttg ttatgacgga accagccgtc gccgccgccg agcgggtggc
      361 gatcgtggac agttcgggca aggtggtttc ttcggacgat gtccggggcg acttgcacta
      421 ggattgcatc cacgcggccg ccgacggacg tgcagcgctc gattccgtcg gcgttggacg
      481 cgtgaggctt ggggcgcgct acggcgccag ttcccgagtc ccacgctgtc caatgaagaa
      541 ccggtgtgtg tcatgcgctt caatacggcc tgtggagaag gcaggtcgct gcgcaaactg
      601 gttgttcact taggtcgagt tgcggacgga tcggggtgtg ggaacggtcg ttacggtcac
      661 cgttggcacc agttcgatgt caacgctccg ccagcgagag aaaacccatg accccgaatt
      721 tcgaaagcca ggaatgcccc aatgattcga ccgggagcca gcgcggccgc tttcagaacg
      781 gagcgcctgt ccacgtgctt gtcgtcgacg attcgcgccc gtcggcagac gctctgggcg
      841 catacctgca agccggcggc atgagcgtga gaaccgtcta ccacgggtcg gccgccctga
      901 gggaagcgaa ggcgtggata cctgatcgca ttgttctgga tgtcgccatg cccggcctat
      961 ccggcatcgg cgttgcagcc gcgcagcgga gcatcgaggc gaccggaaag attccccttc
     1021 tggcattcac cgcgttcgat accgccgact acatccgtga aatgaaggag gttggtttcg
     1081 acgccgtgtg ccgcaagcct tccgaactag accagctcga aggcatgatc ctcaagctgc
     1141 tcggccagaa cgcagtcgat ggctttcaga tcgtgccatc caccgcggag cctgggccgt
     1201 ccgatccggg cctggacacg gttgatcgac attagcgcag agtcaagacg gacgcgacgt
     1261 tctcaattgc gccgcgcccg gtacgacctt ttcgacagtc agcgccgtag aacgtcagga
     1321 tgccccgacg atcgtcggca cgtcggcgcc taatgccaga acagcgcgat cagaataatg
     1381 atgggaatgg gtaccccgag catataaaga agaattgagc gcatagacac ctccttcggt
     1441 aaatgaattg aagtttcaat cagagttggc gtacgcggcc gccatgagtt gccatcagac
     1501 tcgcagagaa tgcgcccatc agaagcgaga tgaataacca caacgatgcg ccgactgtta
     1561 ctttgcgcgc cttgtcggcg gcatctttag cggcagcatc aagggcagtg attttctgca
     1621 gcaggcgcgt gtaggtggtc accacacgag cttgtgctgc ttccggcgta aggccagtat
     1681 gctgcgcgac aagctggctt aaataggcag tatcttccgg cgacaaatga tcgcccgttg
     1741 ccgcgctgtt aaggaatatc cgcgccgttt ccgcgcgggc cgtgtcgttg cggccttccg
     1801 gggtggcggg tgcggcgagt gacgaaccgg aggggttccg gaacagcgaa tcgacgaagt
     1861 agccgagcgg ccatgtgttc gtggcggcct ctgcattgcc acgttgcatc gttcccatcg
     1921 caaccgagcc gcctgccgcg gcagttgtcg cggcggtctg cgcgccggtc cgcacaatgc
     1981 ccgagacggc cgatgtcagt atggcggccg tgaagagcgt cgccacggcc cagctcagga
     2041 aaccatgcgc cgtgtcccgg aagtgaattt catcggcgtc gactgccagc cagcggctgc
     2101 gtagccgtcc cgccagatag ccgcctaatc ccgacgtgag aatcgaggtg acgcaaatcc
     2161 acaccacggc ggcaaagccg aacgcttttg cattagaggg tcccgatgac cacggggaga
     2221 tcgacgtcag gccgaggccg gtgcccaatg tcagaaggat gagtgcgaac gcggccgcac
     2281 cgatgccgcc agcgaacacg gctccccatg agaccgcggc ctcgtgttgc cgcactaaca
     2341 gcgcaggatc tttcgcggcc agcggatagt ccgctggatc tcttgtgtgg ttcattgaca
     2401 gcctccatga ccttgtcgat accggcatcg tccatatgcc gatgcgttcg gcatcgcggc
     2461 gatgccgtgc gggataaatc gtttgcgtgg gacgcatttt ttgtgccgct caggttccgt
     2521 tttccaactc cgcgtcctgg ccgtccgttc ataggcatag ggtttgctcg atgaagcggc
     2581 ggagccgcag cctttgactg tggtcaaacc tgaaacaaga aatacatgac gctagagcag
     2641 cggcttcgca gcatgcgcga gacgatgcgt gacatgttcg gcatcgcccg attgcgcgct
     2701 ggcaaggaag agatcatttg cagcgtgctg gaacggcgcg acacacttgc aacgatgccg
     2761 acaggcgccg gccaatccct ctgttaccag ttgccggcat tacatctcga tggcacgacg
     2821 ctcgtcgtat cgccgctcat cgccatgatg aaggaccagg ccgacaaact gctggcggcg
     2881 ggcgtcgact gtactttggt gaacagctcg tggcgcagcc gcgaagaacg ggaggcgttg
     2941 caacgcattc tggatggaaa gggcggcatc gtttttgtca cgccggagcg gctggcgcag
     3001 cctgcattca tgaaggtatt gcgggcgggt gccggacata gggtgggtct ggtggtggtc
     3061 gacgaagcgc attgcgtgtc gcaatggggc catgacttcc ggccggcgtt tctcgagatt
     3121 gccagcgccg tcaaggcgca cggccaaccg ccggttcgcc gtgcaggtga gggttggtgg
     3181 cggcaaaaat ggcgccgcgc gggccgcaca ggccaagcgc gagcagctgc gcgaagtgat
     3241 cgcgtcgatc acggggacgg gcatcatcta cacagcgacg gtacgcgacc cagacgagat
     3301 ttacgcctgg ctcgcagaag cgggcgagtc cgtgtcgcgt tatcacggca aactggcgcc
     3361 gagcgcgcgc gaagccgctt caggaaacat tcatgtcggg agcgacaagg ttaatggtgg
     3421 ccaccaacgc gttcggcatg ggtattgaca aatccgatat cccttcgtcg tccattacca
     3481 gatgccgggc agccttgacg cctactacca ggaaaccggc cgcgcgggcc gcgacgggca
     3541 acccgccgat tgcgtgctgc tattcggtct gaacgcctcc agcttgccgc cgcccacctc
     3601 gggcaatgcg tccttcagtt ccacgagcgt gacaccttcc ggcgcccgtt tacagcgtgc
     3661 cacgagcgca tcgtacaaac gccacgccag ttcaacgctc gatacctgcc ggcgagaaag
     3721 aactgctgga tgcgccggtc gcgggcacgt aacgacacgc aggatagcgt gcatgatgca
     3781 gtggcgaatg ccgccgctca attcgaagca atgagcgtcc acgacaagga aacgctgcaa
     3841 cggatgatcg actacgcgca gaccggcgaa tgccgttggc gcgcgatact cgcgcattac
     3901 ggtgatacgc cgtcgatgca gcgctgcggc gtctgcgaca actgcatgaa tccaccgtac
     3961 attgcgctgg cggagcagtc gatcgccgtg gacgacacgc tgcgcgacgc ggacgcatcg
     4021 aaaccatcgt cgaggccgc
//
LOCUS       DATASH010000791         4619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4878550,
            whole genome shotgun sequence.
ACCESSION   DATASH010000791 DATASH010000000
VERSION     DATASH010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4619
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4878550"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /gene="mraW"
                     /locus_tag="VHV99_21205"
     CDS             <1..292
                     /gene="mraW"
                     /locus_tag="VHV99_21205"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012402052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HEX3637951.1"
                     /translation="ELAELQVVLEAALSLLEQGGRLVVISFHSLEDRIVKRFMQAHAS
                     TPAVDRRLPIRAVDLPSPPLKIIGRVFASDAEVAANPRARSAVMRVAERIAP"
     gene            289..639
                     /gene="ftsL"
                     /locus_tag="VHV99_21210"
     CDS             289..639
                     /gene="ftsL"
                     /locus_tag="VHV99_21210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEX3637952.1"
                     /translation="MSRLNIFLLMILMGCALSVVNATNQQRQIFIQLQRAQSQERQLQ
                     QDYSQLQYQQSAQSKTSRIEQIATDSLKMQAVSTGRTQYLTLDPGAAKAEDAPIPTSA
                     PASAPVATRRGGVR"
     gene            636..2501
                     /locus_tag="VHV99_21215"
     CDS             636..2501
                     /locus_tag="VHV99_21215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX3637953.1"
                     /translation="MKKSSTRKSVAFSANPILSVRLPMWRSKLVVFMLFMAFVALTAR
                     AFWIQGPGNAFYQKQGEIRYQRRLELPATRGKILDRNGLVLATSLPVRAIWAIPESVP
                     DDLGADKLNQLGKLLDMTNTELRAKLSEDKNFVYVKRQVPVDVAAKVAALDIPGIYSR
                     DEYKRFYPEGEITAHLVGFTNVEDEGQEGVELGDQKLLAGLSGSRRVIKDRMGHIIED
                     VDEQVVPHNGQDVDLSIDSKIQYIAYTNLKAAVEKFKAKAGAAMVVDVRTGEVLALVN
                     YPTYNPNDRTHLTGDQLRNRILTDTFEPGSIMKPFTVSLALDLHRVTPTTLVDTGSGR
                     FVLDGAPITDDSGFGVLTVGGVIQKSSNIGATKIAMQLKPEEMWNMYTSIGLGQAPKV
                     GFPGAAAGRLRPWKSWRRIEQATMSYGYGLSVSLFQLGRAYTAIAHDGQIMPVSIFRT
                     PGDQPATGPQIFAPTTAREMRAILETVTAPGGTSPDAAVPGYRVGGKSGTAYKHGAHG
                     YDHSKYRASFVGMAPMPNPRIVVAVSVDEPTAGSHFGGQVSGPVFSSIVGDTLRSLNV
                     PPDMPVKQMVVSDDSSPAAPGAPPSAATVKKLSTSVGAKKMTIAANAKSHPGVVR"
     gene            2498..4090
                     /locus_tag="VHV99_21220"
     CDS             2498..4090
                     /locus_tag="VHV99_21220"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015602111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEX3637954.1"
                     /translation="MSALRKKHPAHRQIADALNWLHARVQPGAHLHADTRSLAAGDAF
                     FAYAVDGADNRPFIDGAIERGAAAVLVQPEAFSGAIDPSTTLAVPALNELAGSIASAW
                     YSDPSDAMLSIGITGTNGKTSCSQWISAALTALGKRCAIIGTLGTGLPGQLVHTGFTT
                     PDAPQLQRSLAQLRDAGAQAVAMEVSSHALHQGRVNGTAFDIAVFTNLTQDHLDYHGT
                     FEAYEAAKARLFAWPGLRAAVINRDDAAGRRLLASTKGHARTFAYGLDEALKDAPQGN
                     APGVDASGAAAPQADALLLASNVRATATGTAFHLTTSDWGNAEVEVQTLGAFNVSNLL
                     GVLGALLAAEVPFDAALAELAKLESVNGRMQRLGGRLQNDEPLVVIDYAHTPDALEKT
                     LEALRPMAAARGGELICIFGCGGDRDATKRPLMGAIAEKLADCVVVTSDNPRSEDPQS
                     IIEQIAAGMKDASKARRVEDRASAILQAIRGAAREDVIVLAGKGHEATQEIMGKKRAF
                     SDQDHARLALAARATHARGGGE"
     gene            4087..>4619
                     /locus_tag="VHV99_21225"
     CDS             4087..>4619
                     /locus_tag="VHV99_21225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX3637955.1"
                     /translation="MTQPGAAMFSLREAAALIPGATVLGDDSAVFERVSTDSRSAGPG
                     DLFVAIKGDRFDAHDFLPEVASRNVAAVLVSRTPDALRVPALRVTDTRAGLGALARGW
                     RRKFSMPLVAVTGSNGKTTVKEMIASIFAAAVGADACLATAGNFNNDIGLPLTLFRLH
                     TAHQLAVVELGMNHPGET"
BASE COUNT          854 a         1516 c         1478 g          771 t
ORIGIN      
        1 agagcttgcg gagctgcaag tcgttttaga agcagcgttg tcgctgttgg agcaaggggg
       61 gcggctggtg gtcatcagct ttcattcgct cgaggaccgg atcgtcaaac gattcatgca
      121 ggcgcacgcc agtacgcctg cggtggatcg ccgtctgccg attcgcgcag tcgatctgcc
      181 gagccctccg ctcaaaatca tcggccgcgt gttcgcaagc gacgctgaag tcgccgcgaa
      241 tccgcgtgcc cgttctgccg tgatgcgcgt ggcggagcgg atcgcaccat gagtcgcctc
      301 aacatcttcc tgctgatgat cctgatgggc tgcgctttgt ctgtcgtcaa tgcaaccaac
      361 cagcagcgtc agatctttat ccagttgcag cgcgcgcaat cgcaggagcg ccagctgcag
      421 caggattatt cacagcttca atatcagcag agcgcacagt cgaagacgtc gcgcatcgag
      481 cagatcgcca ccgattcgct gaagatgcaa gcggtcagca ccggtcgcac ccagtacctg
      541 acgctcgatc cgggcgcggc caaggctgag gacgcgccga tcccgacttc cgccccagcc
      601 tcggcgccgg tggcgacccg tcgcggaggc gtgcgatgaa aaaatcgtcg actcgcaaga
      661 gcgtcgcctt ttcggcgaac ccgattctgt cggtgcgcct gccgatgtgg cgctcgaaac
      721 tcgtcgtgtt catgctcttc atggcgttcg tcgcgctgac cgcgcgcgcc ttctggattc
      781 agggtccggg caacgcgttc tatcagaagc agggcgaaat ccgctatcag cgccggcttg
      841 aactgccggc cacgcgcggc aagattctcg accgcaatgg cctcgtgctc gccacgagct
      901 tgccggtgcg cgcgatctgg gccattccgg aatccgtgcc cgacgacctc ggcgcggaca
      961 agctgaacca gctcggcaag ctgctcgaca tgacgaacac ggagttacgc gccaagctgt
     1021 cggaagacaa gaacttcgtc tacgtgaagc gccaggtgcc ggtcgacgtt gccgcaaaag
     1081 tcgcggcact ggacattccc ggcatttact cgcgcgacga gtacaagcgc ttctatccgg
     1141 aaggcgagat cacggctcac ctggttggct tcaccaatgt cgaagacgaa ggccaggaag
     1201 gcgtcgaact cggcgaccag aaattgctgg ccggcctgtc gggcagccgc cgcgtcatta
     1261 aagaccgcat gggccacatc atcgaggatg tggacgagca ggtggtgccg cacaacggcc
     1321 aggacgtcga tctctcgatc gacagcaaga tccagtacat cgcgtatacg aatctgaaag
     1381 cggccgtcga gaaattcaag gcgaaggccg gcgcggcgat ggtagtcgat gtgcgcaccg
     1441 gcgaggtgct ggcgctggtc aactatccga cgtacaaccc gaacgaccgt acgcacctca
     1501 cgggcgacca gttgcgcaac cgcattctca cggacacctt cgagccgggt tcgatcatga
     1561 agccgttcac ggtgtcgctc gcgctcgacc tgcatcgcgt cacgccgact acactggtag
     1621 acacggggtc gggccgcttt gtgctcgacg gtgcgccgat taccgacgat agcggtttcg
     1681 gcgtgctgac ggtggggggc gtgatccaga agtcgagcaa catcggcgcg acgaagatcg
     1741 ccatgcagct caagcccgaa gagatgtgga acatgtacac cagcatcggt ctcggccagg
     1801 cgccgaaggt cggcttcccg ggtgcggcgg cgggccgtct gcgtccgtgg aagagctggc
     1861 gccgcatcga acaggcgacg atgtcgtacg gttacggctt gtcggtgtcg ctgttccagt
     1921 tgggccgtgc gtacaccgcg atcgcgcatg acggccagat catgccggtg tcgattttcc
     1981 gcacgcctgg cgatcaaccg gcaacggggc cgcaaatttt cgcgcccacc actgcccgcg
     2041 aaatgcgcgc gatcctcgaa acggtgacgg cgccgggcgg cacgtcgccg gatgcggccg
     2101 tgccgggcta tcgcgtcggc ggcaagagcg gtaccgccta caagcatggc gcccacggct
     2161 acgaccactc caagtaccgc gcatcgttcg tcggcatggc gccgatgccg aatccgcgca
     2221 tcgtcgtcgc cgtgtcggtg gacgaaccga cggcgggcag tcactttggt ggtcaggtgt
     2281 cgggcccggt gttctcttcg atcgtcggcg atacgctgcg ctcgctgaac gtgccgccgg
     2341 acatgccggt caagcagatg gtggtgtccg acgattcgtc gcctgccgca ccgggcgcac
     2401 cgccttcggc cgcaacggtc aagaaacttt ccaccagcgt cggcgcaaag aagatgacga
     2461 tcgccgccaa cgcgaaaagc catcccggag tcgtgcgatg agcgcgctgc gcaagaagca
     2521 cccagcgcac cggcaaatcg ccgacgcgct caactggctg cacgctcgcg tgcagccggg
     2581 cgcgcatctg catgccgaca cacgttcgct cgcggccggc gatgcgtttt tcgcctacgc
     2641 ggtcgacggt gcggacaacc gcccgttcat cgacggcgca atcgagcgtg gcgcggcagc
     2701 cgtgctggtc cagcccgaag cttttagcgg cgcgatcgat ccgtccacga cgctggccgt
     2761 gccggcgttg aatgaactcg ccggttcgat tgcgagcgcc tggtatagcg atccgagcga
     2821 tgcaatgctg tcgatcggca tcaccggcac gaacggcaag acttcgtgca gccaatggat
     2881 ttccgcggcg ctgaccgcgc tcggcaaacg ttgcgcgatc atcggcacgc tcggcacggg
     2941 tctgccgggt cagctcgtcc acaccggctt cacgacgcca gacgcgccgc aactgcaacg
     3001 cagtctcgcg cagttgcgcg acgccggtgc ccaggcggtg gcgatggaag tgtcgtcgca
     3061 cgcgctgcat caaggccgcg tgaatggcac tgcgttcgat atcgccgtgt tcacgaatct
     3121 cacgcaagac cacctcgact atcacggcac gttcgaagcc tacgaagccg ccaaggcgcg
     3181 tttgttcgcc tggccgggac tgcgcgccgc ggtgatcaat cgcgacgatg ccgccggtcg
     3241 ccgtctgctc gcgagcacaa agggccatgc acgcacgttc gcctacggcc tcgacgaagc
     3301 gctcaaagat gcgccgcaag gcaatgcgcc gggcgtcgat gcgtcgggcg ccgccgcgcc
     3361 gcaagccgat gcgttactgc ttgcctcgaa cgtgcgcgcc acggctaccg gcaccgcctt
     3421 tcacctgacc acatcagact ggggcaacgc cgaagtcgaa gtgcaaacgc tcggcgcatt
     3481 caacgtcagc aatctgctcg gtgtgcttgg cgcattgctc gccgccgagg tgccgttcga
     3541 cgccgcgctt gccgaactcg cgaagctcga atcggtcaat ggccgcatgc aacgtctggg
     3601 cggccgcttg cagaacgacg agccgctcgt cgtaatcgac tacgcgcaca cgccggacgc
     3661 gctggaaaag acactcgaag ctctgcgccc gatggcagcc gcacgcggcg gcgaactgat
     3721 ctgcatattc ggctgcggcg gcgaccgcga tgcgaccaaa cgtccgctga tgggcgcgat
     3781 cgccgagaag ctcgcggact gcgtggtcgt caccagcgac aacccgcgca gcgaagatcc
     3841 gcaatcgatc atcgagcaga tcgccgcggg gatgaaagac gcgtcgaagg cgcgccgcgt
     3901 cgaggaccgc gcaagcgcga tcctgcaagc gatccgcggc gccgcacgtg aagacgtcat
     3961 cgtgctggcc ggcaaggggc acgaagcaac acaggaaatc atgggtaaga agcgcgcttt
     4021 ctccgatcag gaccacgctc gccttgcgct cgccgcacgg gcaacacacg cacgcggagg
     4081 tggcgaatga cccagccagg tgccgcgatg ttctcgctgc gtgaagccgc cgcgctgatc
     4141 cccggcgcga ccgtgctggg tgacgacagc gccgtgttcg aacgcgtgtc gaccgatagc
     4201 cgcagtgccg gtcccggcga tctgtttgtc gcgatcaaag gtgatcgttt cgacgcgcac
     4261 gatttcctgc cggaagtcgc ctcgcgcaac gtcgcggctg tgctggtctc gcgcacgccc
     4321 gacgctttgc gcgtgcccgc tttgcgcgtc accgatacgc gcgcgggcct cggcgcgctc
     4381 gcacgcggct ggcggcgcaa attcagcatg ccgctcgtcg ccgtgacggg cagcaatggc
     4441 aagaccacgg tcaaggaaat gatcgcgtcg atcttcgccg ccgcggtcgg cgcggacgca
     4501 tgccttgcca ccgccggcaa cttcaacaac gacatcggct tgccgctcac gctgttccgt
     4561 ctgcataccg cgcatcaact agcggtggtc gaactcggca tgaaccatcc gggcgagac
//
LOCUS       DATASH010000792         2414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4879107,
            whole genome shotgun sequence.
ACCESSION   DATASH010000792 DATASH010000000
VERSION     DATASH010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4879107"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..2373)
                     /locus_tag="VHV99_21230"
     CDS             complement(139..2373)
                     /locus_tag="VHV99_21230"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEX3637956.1"
                     /translation="MAINFENRYTDVSGTDELHLSDYLRTIVRGWRTIVMVTLIALAL
                     GCAYAFLAPPTYRADVLFHVEDKTANANANGKDSLPPLTGMFDTKPSTAAEIELLKSR
                     LVTEETVKKLHLDLTAGPRYFPFIGGMIAGLVNGQWGFKLPQFVNLSGYAWGDESISV
                     SQFDTSKDVYDTTFTLISSNDGSYVLRDKNGIAILSGKVGETVQTDTADGPITLRVDK
                     LVGAAGSRFELQRASTLSTVDRLQKALVVQETTLQSGVIRASLEGGDSGLTAAIVNSM
                     AREFVRQDVESRSTEAEHMLAFLDQQLPGLRKELDDAEQRYNKFRNTHGTVDLGEESR
                     LLLQQIVDNKTKLMDLQQQRAEMSQRFTANHPTVASLDAQIAALQGAAATMNRSVAVM
                     PDTEQTALRLLRDVHVDTELYTNLLNSAQQLRVAKAGQVGNVRVVDFAEAPDEPVRPK
                     RVIAILIALGGGLVLGVLLTFFKRAMYGGVERPDELEGMLGVPVFAVVPRSQTQLKLQ
                     ENVMLRRRGLHVLAQQAPEDIAVEGVRNLRTSLQLSLDHAENNVVMITGSRPDSGKSF
                     LSVNLAALVASANKRVLIIDGDMRRGDVHSHFGVAHQPGLSDVLSGGDLSSMIQRDVL
                     PGLDVLAKGTLPSHPAELLMSKRFELMLEELKAQYDLVIVDTPPVLAVTDSTVIGKYA
                     GTTLLVVRHGRHPLNEVMETAKRLRNGGVGLRGVLLTDVPQEGAFLGSGYQGGYYGYD
                     SIAG"
BASE COUNT          419 a          807 c          719 g          469 t
ORIGIN      
        1 ccaggtacga tccgtcctgt ccagtgatgc cggtgatcag cgcgacctta cgtttcattg
       61 aaccttctcc tcgttaacct ttgcaatgcc aatccaccac ccggttgagc ctttgttgcg
      121 cggccggcgg ccgctgtctc aaccggcgat gctgtcgtag ccgtagtaac cgccctgata
      181 acccgagccg aggaacgcgc cttcctgcgg cacatcggtc agcagcacgc ctctgagccc
      241 cacgccgccg ttacgcagcc gcttggccgt ctccatgacc tcgttgagcg gatggcggcc
      301 atggcgcacc accagcagcg tggtgccggc gtacttgccg atcaccgtcg agtcggttac
      361 cgccagaacc ggcggcgtgt cgacgatcac gaggtcgtac tgcgccttca gttcttcgag
      421 catcaattcg aagcgcttgc tcattagcag ttcggccgga tgcgagggca gcgtgccctt
      481 ggcgagtacg tcgaggcccg gcagcacgtc gcgctggatc atcgagctca gatcgccgcc
      541 gctcagcacg tcggacagac ccggctgatg cgcgacaccg aaatgcgagt gcacgtcgcc
      601 gcgccgcatg tcgccgtcga tgatcagcac gcgcttgttc gccgacgcca ccagcgccgc
      661 cagattcacc gacaggaacg acttgccgga atcgggccgc gagccggtaa tcatcacgac
      721 gttgttctcc gcgtgatcga gcgagagctg caacgaggtg cgcagattgc gcacgccttc
      781 gacggcgatg tcttccggtg cctgctgcgc gagcacatgc agcccgcgac ggcgcagcat
      841 gacgttttcc tgcagtttta actgggtctg gctgcgcggc acgaccgcga acaccggcac
      901 gccgagcatg ccttcgagtt cgtccgggcg ttccacgccg ccgtacatcg cgcgcttgaa
      961 gaaggtcagc agaacgccga gcacgaggcc gccgccgagc gcgatcagga tcgcgatcac
     1021 gcgcttcggc cgcaccggtt catccggtgc ttcggcgaag tcgaccacgc gcacgttgcc
     1081 cacctgtccc gccttcgcca cgcgcagttg ctgcgcgctg ttcagcagat tggtgtacag
     1141 ctccgtgtcg acgtgcacgt cgcgcagcag acgcaatgcg gtctgctccg tatccggcat
     1201 gaccgccacg ctgcggttca tggtggccgc cgcgccttgc aaggcggcga tctgcgcatc
     1261 gagcgaggcc accgtcgggt gattcgccgt gaagcgttgc gacatctccg cgcgttgctg
     1321 ctgcagatcc atcagcttgg tcttgttatc gacgatctgt tgcagcagca ggcggctttc
     1381 ctcgcccagg tcgaccgtgc cgtgcgtgtt gcggaacttg ttgtagcgtt gctcggcatc
     1441 atcgagttcc ttgcgcaagc ccggcagttg ctgatcgagg aaggccagca tgtgctcggc
     1501 ttcggtcgaa cggctctcca cgtcctgccg cacgaactcg cgcgccatgc tgttgacgat
     1561 cgccgccgtc aagccgctgt cgccgccctc cagactcgcg cggatcacgc cggattgcag
     1621 cgtggtttcc tgcaccacca gagccttttg caggcgatcc accgtgctca gcgtcgaggc
     1681 ccgctgcaat tcgaaacgcg agccagctgc gccgaccagc ttgtcgacgc gcagcgtgat
     1741 cggcccgtcg gcggtatccg tttgcacggt ttcgcccact ttccccgaca gtatcgcgat
     1801 accgttcttg tcgcgcagca cgtacgaacc gtcattgctg gaaatcagcg tgaaggttgt
     1861 gtcgtacacg tccttcgacg tgtcgaactg cgacaccgaa atgctctcgt caccccatgc
     1921 atagccggac aggttgacga actgcggcaa cttgaagccc cattgcccgt tcaccaaccc
     1981 cgcaatcatt ccgccgataa acggaaaata gcgcggccca gccgtgagat cgaggtgcag
     2041 cttcttgacg gtttcctccg tgacgagacg cgacttcagc aactcgatct cggccgccgt
     2101 cgaaggcttg gtgtcgaaca tgccggtgag cggcggcagg gaatccttgc cgttcgcatt
     2161 ggcattcgcc gtcttgtcct cgacgtgaaa caagacatcc gcgcggtacg tcggaggcgc
     2221 gaggaaggcg tacgcgcacc ccagcgcgag cgcgatcagc gtcaccatca cgatcgtgcg
     2281 ccaacctcgc acgatcgtac gcagatagtc cgacagatga agctcgtccg ttccggacac
     2341 gtcggtgtag cggttttcga agttgattgc cattttcttc acccaacaaa gcccggcggt
     2401 tcaagatcac gttg
//
LOCUS       DATASH010000793         6787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_924615,
            whole genome shotgun sequence.
ACCESSION   DATASH010000793 DATASH010000000
VERSION     DATASH010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6787
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924615"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..93
                     /locus_tag="VHV99_21235"
     CDS             <1..93
                     /locus_tag="VHV99_21235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3637957.1"
                     /translation="RKTMLSVFDLAAHQPELVSQWRRKLSALLF"
     gene            complement(222..1079)
                     /gene="tcdA"
                     /locus_tag="VHV99_21240"
     CDS             complement(222..1079)
                     /gene="tcdA"
                     /locus_tag="VHV99_21240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HEX3637958.1"
                     /translation="MSSTTAHSDLTTGLDGSETADRARRFGGIARLYGGPALAAFEGA
                     HVAVIGIGGVGSWVAEALARSAVGTLTLIDLDNVAESNTNRQIHALDGNYGKPKVEAM
                     AERIAAINPFCDVRLIEDFVEPDNFAATLGDGFDYVIDAIDSVRTKTALIAWCVEKKQ
                     PLITVGGAGGQLDPTRIRIDDLALTIQDPLLSKVRGQLRKQHGFPRGPKAKFKVSAVY
                     SDEPLIYPEAAVCDIDEEAEHVTTSPGHTGPVGLNCAGFGSSVCVTASFGFAAAAHVL
                     RALAKQAAG"
     gene            1312..1953
                     /gene="pdxH"
                     /locus_tag="VHV99_21245"
     CDS             1312..1953
                     /gene="pdxH"
                     /locus_tag="VHV99_21245"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012399931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEX3637959.1"
                     /translation="MTSLAELRKNYSLGSLDIADIDRNPFRQFDTWFQQAVDAQLPEP
                     NTMTLATVDSRGRPSARIVLIKGVDERGFVFFTNYESRKGRELAANPYASLLFYWIEL
                     ERQVRVEGSIVKTSPEESDTYFASRPLGSRIGAWASNQSQVIESRSQLETREREISLQ
                     YGDQPPRPPHWGGYRLVPEAIEFWQGRPSRLHDRLLFTRSGENSDWQISRLSP"
     gene            2095..3309
                     /locus_tag="VHV99_21250"
     CDS             2095..3309
                     /locus_tag="VHV99_21250"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEX3637960.1"
                     /translation="MFWEKKLTQWVEDVKTKANLPARLVLWDGQQHDFGQFAAPQVTL
                     HVKSATALPYLLEPSLDNLGEAYVKGKIDIEGKLSDIINIGYQLARNTVTSASKLARV
                     RRYFNHSKSSDKKAIQYHYDVSNEFYKLWLDENMVYSCAYFENGDEDLATAQIKKIDH
                     ILTKIQLQPGQRLLDIGCGWGALVLRAAQKFGAKCVGVTLSQNQFDLATARVKAAGLE
                     SQIEIRLQDYRDVVGQFDRITSVGMFEHVGRKNLPGYFEKIRDLLVDDGVAMNHGITS
                     SDSDSGETALGGGEFIDRYVFPDGELPHISLALEAMQRGGLEAVDVESLRRHYAHTLD
                     LWAENFEAHADEARKLVDDEKFRIWRVYLAGCAYAFETDDVSIYQVVCRKAGRSAKTL
                     PWSRRYMYDKTL"
     gene            3412..4899
                     /locus_tag="VHV99_21255"
     CDS             3412..4899
                     /locus_tag="VHV99_21255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX3637961.1"
                     /translation="MDQSGTSEVEQPASGEHDAQADAVQFDLFGMPVETSATPLKADA
                     SVRGQRASAGGGGGAGAGTETAAGADTGAGADTETTTKVALTSSAAMPTSAATAPDER
                     RRQSRAKASGAALLWQEDDAPVPPEAETTTDAKANTAPATADTPKKRRTRDVLAAPPS
                     AELLALAADLPPRVHLGTSTWSFPGWNGIVYGDDYSNSKLSREGLTAYAAHPLLRTVS
                     IDRSFYQALTVTEYLRYAQQVPEHFRFIVKAPMTITDATVRAERGEPVSLNPCFLNAQ
                     MAIDEFVTPCLEGLGAKAGALVFQLSPLPDQMLAQPAAFIERLAGFLMALPKLPDGTC
                     YAIEIRDASLLTPRFIRTLKTAGVRYCVGIHARMPDPLRQAAALALLDGEPAGPLIVR
                     WSLHGGFKYEQAKAKYEPFNQLVDQDPTTRAALAELAARYALAGQTVVIAINNKAEGS
                     APLSCVELATSIIDAYARLAHEYEHEPQLPAEGLQQEDGERGEPA"
     gene            complement(4925..5473)
                     /gene="msrA"
                     /locus_tag="VHV99_21260"
     CDS             complement(4925..5473)
                     /gene="msrA"
                     /locus_tag="VHV99_21260"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX3637962.1"
                     /translation="MSQTGEVATLGGGCFWCLEAVYLGVEGVNSVESGYAGGQTQRPT
                     YEQVCDGVTGHAEVVNVDFDPAKIGYREILDIFFAIHDPTQLNRQGNDVGTQYRSVIF
                     THSDAQRETALQAIREIGEQQIYDGQIVTQVLPLDGNYWPAEAYHQNYFAQHPNQGYC
                     SFVVAPKVAKFRQKFAHRIKAS"
     gene            5730..6239
                     /locus_tag="VHV99_21265"
     CDS             5730..6239
                     /locus_tag="VHV99_21265"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX3637963.1"
                     /translation="MKRASPPNFDQAAFKQALSQFATGVTVITTRAASGQLIGITASS
                     FNSVSLNPPLVLWSLASRSASMPVFRANSHYVVNVLAASQLDLCKRFATVKGDRFEGV
                     SHAEGDTGMPVLDGALAWFECHNRSRYEEGDHVIFVGEVERCGVHENAAEIAPLVFQN
                     GLFHGLKPL"
     gene            complement(6342..>6787)
                     /locus_tag="VHV99_21270"
     CDS             complement(6342..>6787)
                     /locus_tag="VHV99_21270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX3637964.1"
                     /translation="ISNLDLAERISLSPSACLRRLRLLEEQGVIEHYRACLNREVLGF
                     ELEAFVQVSMRNDQENWHERFAEAVRDWPEVVAAFVVTGETHYLLRVLAHNLKHYSDF
                     VLQRLYKAPGVMDIRSNIVLQTLKEDSGVPVSLVTKTSGHSASHN"
BASE COUNT         1375 a         2155 c         2011 g         1246 t
ORIGIN      
        1 cgtaagacca tgttgtcggt gttcgacctc gccgcacatc agccggagct ggtgtcgcaa
       61 tggcggcgca agctgagcgc gttgttgttc tgatttgtat tgtgttcagg tgcggcgagg
      121 gtgccgcgcc tgaatgcgca gtgacatgaa ctgcagcaac gcgcgccctc gcaatagctc
      181 aatggcttaa tgcctgacgg cccgcgtcac gccctaccgc ttcaacccgc tgcctgcttt
      241 gccaacgccc gcaacacatg cgcggccgcg gcaaaaccga agctcgcagt cacacacacg
      301 ctcgagccaa atcccgcgca gttcaaaccg accggtcccg tatgacccgg cgacgtggtg
      361 acgtgctccg cttcctcgtc gatatcgcac acggcggctt ctggatagat cagcggctca
      421 tcggaataca ccgcgcttac cttgaacttc gccttcggcc cacgcggaaa accatgttgt
      481 ttgcgcagtt gtccgcgtac tttcgacagc aacgggtcct ggatagtcaa cgcgagatcg
      541 tcgatacgaa tgcgcgtcgg atccagttgg ccacccgcac cgcccaccgt aatcagcggc
      601 tgcttctttt cgacgcacca cgcgatcaac gcggtcttgg tgcgtacgct gtcgatcgca
      661 tcgatcacgt aatcgaaccc atcgccgagc gtcgccgcga aattgtccgg ctcgacgaag
      721 tcttcgatca gccgcacgtc gcagaacgga ttgatggccg cgatgcgttc ggccatcgcc
      781 tcgaccttcg gttttccgta gttaccgtcc agtgcgtgga tctggcggtt cgtgttgctc
      841 tcggcgacgt tgtcgagatc gatcaaggtc agcgtgccga ccgcgctgcg cgccaacgcc
      901 tccgcgaccc acgagccgac cccgccgatg ccgatcacgg cgacgtgcgc cccttcgaac
      961 gcggcgagcg ccggtccgcc gtacaagcgg gcaatgccgc cgaagcgccg cgcgcggtcg
     1021 gcggtttcac tcccgtcgag gccggtagta agatcggaat gcgcggtggt gctggacatg
     1081 gtgggaggct cgttcaaaac aggcaaaaca cagagtggtc acgctgaagc gttcgtagcc
     1141 cgtattttgc ctgatcgcgc acatccacat ggccgcacca atctggaggc acgaatcacc
     1201 gcaaacgtgg tgacctggat gtccacacag cggggtgggg cttgacctgg ttgcgcgcaa
     1261 aaaattcacg ccttagctat actaattgga ctgtagcgtt cgcataagaa catgacctca
     1321 ctcgccgaac ttcggaaaaa ctattcgctg ggttctctgg acattgccga tatcgaccgc
     1381 aacccgtttc gtcaattcga cacgtggttt caacaagcgg tagacgccca actgcccgag
     1441 ccgaatacga tgacgctggc cacggtcgac tcgcgcggcc ggccgtcggc gcgcatcgtg
     1501 ttgatcaaag gggtcgacga gcgcggcttt gtgttcttca ccaactacga aagccgcaag
     1561 ggccgcgaac tggccgccaa tccctacgct agcctgctct tctattggat cgagctcgaa
     1621 cgccaggtgc gcgtcgaagg cagcatcgtt aaaacgagcc ctgaagaaag cgatacgtac
     1681 ttcgcgtcgc gcccgctcgg ttcacgcatc ggcgcgtggg catcgaatca gagtcaggta
     1741 attgaaagcc gttcgcagct cgaaacacgc gaacgcgaaa tcagcctgca atacggcgac
     1801 caaccgccgc gcccaccgca ctggggcggt tatcgtttgg tacccgaagc aattgaattc
     1861 tggcagggcc ggccgtcgcg tctgcacgac cgtttgctct tcacgcgttc aggcgaaaac
     1921 agcgattggc aaatctcccg cctatcgcct tgaatccaaa tacaacgccg tatgcgcgtt
     1981 gcccacgcag cagcgcgcga cggccggatc gcgcctcgcg gtatgggagt tgcaccgcgg
     2041 tgagccgcga tccacacagg ctttgcttta gttcaacgga cacggagaga tcacatgttc
     2101 tgggagaaga agctgacgca gtgggtggaa gacgtcaaaa ccaaggctaa cctgccagca
     2161 cgcctggtgc tgtgggacgg ccagcaacac gacttcgggc agttcgccgc acctcaggtc
     2221 acgctacacg taaaaagcgc cacagcgctg ccatatctgc tcgaaccgag cctcgacaat
     2281 ctcggcgagg catacgtgaa gggcaagatc gacattgaag gcaagctgtc ggacatcatc
     2341 aacatcggct atcagctggc gcgcaacact gtcaccagcg cgagcaagct ggcgcgcgtg
     2401 cggcgctact tcaatcattc gaagtcgtcg gacaagaagg ccattcagta ccactacgat
     2461 gtctcgaacg agttctacaa gctgtggctc gacgagaata tggtgtactc ctgtgcgtac
     2521 ttcgagaacg gcgatgaaga cctcgccacc gcgcagatca agaagatcga tcacatcctg
     2581 accaagatcc agttgcaacc ggggcagcgg ctgctcgata tcggctgcgg ctggggcgcg
     2641 cttgtgctgc gcgcagcgca gaagttcggc gcgaagtgcg tcggcgtcac gttgtcgcag
     2701 aaccagttcg atctcgccac cgcgcgcgtg aaagccgcgg gtctggaaag ccagatcgag
     2761 attcgtctgc aggattaccg cgacgtcgtt ggacagttcg accgcattac cagcgtcggc
     2821 atgttcgagc atgtgggccg caaaaatctg ccgggttact tcgagaaaat tcgcgatctg
     2881 ctcgtcgacg acggcgtcgc catgaaccac ggcatcacgt cgagtgattc ggacagcggc
     2941 gaaaccgcgc tgggcggcgg tgaattcatc gaccgctatg tgtttcccga tggcgagctg
     3001 ccacacatca gccttgcgct cgaagccatg cagcgcggcg gccttgaggc ggttgacgtc
     3061 gaaagtctgc gccgtcacta tgcgcacacg ctggacctct gggcggaaaa cttcgaggcg
     3121 catgcggacg aagcccgcaa gctggtcgac gacgaaaaat tccgcatctg gcgcgtgtac
     3181 ctcgccggct gcgcgtacgc gttcgaaacc gacgacgtct cgatctacca ggtggtgtgc
     3241 cgcaaagccg gccgcagtgc aaaaaccttg ccgtggtcgc gccgctatat gtacgacaaa
     3301 acgttgtgat acggttgcgc cgcggcgatg tgccgttgct gcgcgccacg gcgatatgct
     3361 tgcatgagcg ggccgacgcg gcgcaaacct taaacaacag gtcgaacatc gatggaccag
     3421 agcgggacaa gcgaagttga acagccagcg agcggcgagc atgacgcgca ggcggacgcc
     3481 gtgcagttcg atctgttcgg gatgccggtg gagacgtcgg caacgccgtt gaaggcagat
     3541 gcgagtgtga ggggacagcg cgcgtctgct ggtggtggtg gtggtgccgg tgctgggacg
     3601 gagactgcgg ctggagccga taccggagca ggagccgaca ccgaaaccac gaccaaagtc
     3661 gctttaacaa gctcggctgc aatgccaacg tccgctgcca cagcgccgga cgagcggcgg
     3721 cgccagtcac gcgcaaaggc aagcggcgcc gccctgctct ggcaggaaga cgacgcaccc
     3781 gtgccgcccg aagccgagac cacgaccgac gccaaagcca acacagcgcc agcaactgcg
     3841 gacacgccaa agaaacgccg tacccgcgac gttctcgccg ccccaccctc tgccgaactc
     3901 ctggcgctcg ccgccgacct gccaccgcgg gtccacctcg gtacgtcgac atggtcgttt
     3961 ccaggctgga acggcatcgt ctacggcgac gactacagca atagcaaact ctcgcgcgaa
     4021 ggtctgacgg cttatgcggc gcatccgctg ctaagaacag tcagcatcga ccggtcgttt
     4081 taccaggcgc tcaccgtcac cgaatacttg cgctacgcgc agcaggtgcc cgagcacttc
     4141 cgcttcatcg tcaaagcgcc gatgacgatc acggacgcca ccgtgcgcgc cgaacgcggc
     4201 gagccggtct cgctgaaccc ctgctttctg aacgcgcaaa tggcgatcga cgagttcgtc
     4261 acgccatgcc tcgaaggcct cggcgcgaaa gccggcgcgc tggtgttcca gctctcaccg
     4321 ttgcccgacc agatgcttgc gcaaccggcg gcattcatcg agcgccttgc cgggttcctg
     4381 atggcgttgc ccaagctgcc tgatggcacg tgctacgcga tcgaaatccg cgacgcgagt
     4441 ttgctcacgc cgcgcttcat tcgcacgctg aaaacagcgg gcgtgcggta ttgcgtgggg
     4501 attcatgcgc ggatgccgga tccgctgcgc caggcggcgg cgctcgcgct gctggacggc
     4561 gagccggccg gtccgctaat cgtgcggtgg agtttgcacg gcggcttcaa atacgagcag
     4621 gcaaaggcca agtacgagcc gttcaaccaa ctcgtcgacc aggacccgac gacccgcgcc
     4681 gcgctcgccg agctggccgc gcgctatgcg ctggccggcc agacggtggt aatcgcgatc
     4741 aataacaagg cggaaggatc ggcgccgtta agctgcgtgg aactggcgac gtcgattatc
     4801 gacgcgtatg cgcggcttgc tcatgaatac gagcatgagc cgcagttgcc agctgaaggc
     4861 ctgcagcagg aagacggcga acgcggcgag ccagcctagg cctcgccgcc caccaccaac
     4921 ccgctcagct cgccttgatc cgatgcgcaa acttctgacg aaacttcgcg actttcggcg
     4981 ccactacgaa cgagcagtac ccttgattcg gatgctgcgc gaaatagttc tggtgatacg
     5041 cttcggccgg ccagtagttg ccgtcgagcg gcagcacctg ggtgacgatc tggccgtcgt
     5101 agatctgctg ttcgccgatc tcgcgaatcg cctgtaacgc cgtttcacgc tgcgcatcgg
     5161 agtgcgtgaa aatcaccgaa cggtattgcg tgcccacgtc gttaccctgc cggttcaact
     5221 gggtcggatc gtggatcgcg aaaaaaatat cgagaatctc gcgatagccg atcttcgccg
     5281 gatcgaagtc gacgttcacg acttccgcgt ggcccgtcac accgtcgcac acctgttcat
     5341 aggtcggccg ttgggtctgg ccgccggcat agcccgactc caccgagttc acgccttcga
     5401 cgcccagata caccgcttcg aggcaccaga aacacccacc acccaaggtg gcgacttcgc
     5461 ctgtctgact catgctgctt gctccttgat actgatagtc ggccgatgcc ggtcccgctt
     5521 cgtagcaatg ctcattccac aaccgggact ttccagctta gcggtttttc cactaagcgg
     5581 caggggtcgc ggccagtcca ccgcgattgc agttaaaatt gggacaaatc gacgattttc
     5641 actatgactt tcgaatcatt tgtttccaac cgcacgcgtg atgggtttag cctggggttc
     5701 agccggcccg gctccggggc ttccgcccga tgaagcgcgc cagcccaccc aatttcgacc
     5761 aggccgcctt caaacaggcg cttagccaat tcgccactgg cgtcactgtc attacaacgc
     5821 gtgccgcgtc cggccaattg atcggcatca cggccagctc cttcaattcg gtttcgctca
     5881 atccgccgct ggtgctgtgg agtctcgcca gccgctcggc gtcgatgccg gtgttccgcg
     5941 cgaacagcca ctacgtagtc aatgtgctgg ccgcgtcgca actcgatctg tgcaaacgct
     6001 tcgccaccgt gaagggcgac cgtttcgaag gcgtgtcgca cgccgaaggc gacaccggca
     6061 tgccggtgct cgacggtgcg cttgcctggt tcgaatgcca taaccgcagt cgctacgaag
     6121 aaggcgacca cgtcattttt gtcggcgaag tggagcgctg cggcgttcac gaaaacgctg
     6181 cggaaattgc gccgctggtc tttcagaacg gtctgttcca cggactcaag ccgctctgaa
     6241 gacggtgcgg cggcttttta acagcggtct caagcagctg gaaactgcgc tgcgcaacgc
     6301 gcaaagaaac aagccgccgc aagccgctcc gccgctcgca ctcagttatg cgatgcgctg
     6361 tgtccgctgg ttttcgtcac tagcgaaacc ggcacgcctg aatcttcctt cagcgtttgc
     6421 agcacgatat tcgaccgaat atccatcacg cccggcgcct tatagaggcg ttgcagcacg
     6481 aaatccgaat agtgtttgag attgtgggcg agcacccgca gcagataatg ggtctcgccg
     6541 gtcacgacaa acgcggcgac cacttccggc caatcccgca ccgcctccgc aaaacgctcg
     6601 tgccagttct cctggtcgtt gcgcatggac acctgcacga acgcttccag ttcgaaaccc
     6661 agcacctcgc ggttaagaca cgcgcggtaa tgttcaatga cgccctgctc ttccagcagg
     6721 cgcaggcgtc gtagacaggc cgacggcgag agcgaaatgc gctccgccag gtcgagattg
     6781 ctgatcc
//
LOCUS       DATASH010000794        11218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4684367,
            whole genome shotgun sequence.
ACCESSION   DATASH010000794 DATASH010000000
VERSION     DATASH010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 11218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11218
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4684367"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            336..1343
                     /locus_tag="VHV99_21275"
     CDS             336..1343
                     /locus_tag="VHV99_21275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEX3637965.1"
                     /translation="MDYPRIAVGALGGTICMTADNADAGALPRLSAQQLVTAVPGLAD
                     IAQIHAKTLLQLPSPSLNFAHVLDSLAWAERMVAAGAAGIVLTQGTDTLEETAFLLDL
                     LWTHPQPMVVTGAMRTPLAAGADGPANLLAAVACAAHGASRGRGVAVVMNNTIHEARW
                     VRKANSMSVETFESPVVGPAGMVLEGMPQYFRPPSRRYSPRFAKPDLSVRVALVETCL
                     GETGQMLDAVVHAGYHGIVIAAYGAGHVSDDEAQRIEALAPQLPIVISSRTGSGSTAA
                     RTYGFSGSEIDLARRGALFSGWLGPRKARLLLWLLLATGAPRNVCATAFEFWKRVDNA
                     D"
     gene            complement(1444..2346)
                     /locus_tag="VHV99_21280"
     CDS             complement(1444..2346)
                     /locus_tag="VHV99_21280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3637966.1"
                     /translation="MDAVSDLEFFVELVKHGSLSALARDVGVTPPAVSARLAQIERRL
                     GVRLLNRTTRRLSMTHEGEQYLTTGSKLLSALQELDRTISSSRATPKGLLRVNATFGF
                     GRRHIAPAIAAFHRQYPDVEVQLELTDRPLNLAEAAFDVGVRFGEIPDSRLIARKVAT
                     NRRMVCASPIYVRAKGEPDTPRDLQRHQCIVLRENDTAYGTWHFSKGKRHETVKVHGV
                     LSSNDGEATLVWALDGQGILMRSEWDATSYLQSGRLKQLLPDWSLPPADIYAVYPERL
                     NLTAKVTAFIEFLEGFLGKGPGAG"
     gene            2476..3891
                     /gene="leuC"
                     /locus_tag="VHV99_21285"
     CDS             2476..3891
                     /gene="leuC"
                     /locus_tag="VHV99_21285"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008346356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX3637967.1"
                     /translation="MPHTFYQKLVASHTVDRLDEQHVLLYADLHIMNEYTSPQAFAGL
                     AEKDVLVRRPRQQMGIVDHVIPTRAVPVAARTIDIVDASKQAANFTRNCVTQGIHLFD
                     VTDPAQGIEHVVAPEIGLIRPGMVVLCGDSHTTTYGALGALGFGIGTSEVEHVLATQT
                     LVYRVADDMRITVDGELPAGTTSKDLVLHIIARIGAQGARGFALEYAGSTIASLSAEA
                     RMTLCNMTVEAGARAALIAPDDTTIDYVRARVRDLDAATLDSATAAWRSLGSDSDAVF
                     DVEYHVDASQIAPYVTWGTSPDQAIPVDGHIPEVDTATGIDAATARKALDYIALPAGA
                     SIEGTPIDRVFIGSCTNARIEDLRAAAAMVRGRKVAASVRAMVVPGSGMVRDQAEREG
                     VAQILIDAGFEWRQPGCSMCLAMNDDVLAPGERCASTTNRNFEGRQGRGSKTHLMSPA
                     MAAAAAIAGHIVDIRKELVHG"
     gene            3884..4525
                     /gene="leuD"
                     /locus_tag="VHV99_21290"
     CDS             3884..4525
                     /gene="leuD"
                     /locus_tag="VHV99_21290"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008346354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX3637968.1"
                     /translation="MADRRLIQGTAAPLPINNLDTDQIMPKQFLLGIDKSGLARGLLY
                     DLRYDERGQARETFVLNRPEYRDTRILIGGSNFGCGSSREHAVWGLQQAGIEAVIAPS
                     YGEIFYFNAGNNRLLLVTLAPEIVDALVAELAGADTKQLSIDVENEVVVTASGERHPF
                     SIPSRQKRMFMDGLDMVQMTLQQSDQIDAFEKRHWAANPWARIDLPAQQRSTL"
     gene            4629..5960
                     /locus_tag="VHV99_21295"
     CDS             4629..5960
                     /locus_tag="VHV99_21295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016348650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3637969.1"
                     /translation="MPQPSEPANVPDAHALEARTVKKTTWRIVPFIIALYFVSFLNRV
                     NVGFAGLTMNHDIGLTPAMFGFGAGIFFVGYIVFGMPSNLALQRVGARRWIALLMVVW
                     GALSAATACVSGPYSFYVLRFLLGLAEAGFFPGIILYLSYWFPARHRALVTAIFMTAA
                     PLSNMIGSPISGAIMGMDGIAQIHGWQWLFILEAAPTIALGVIAYFFLTDKPEDARWL
                     ATDEREWLTLEMDRERSLKATHAASSVWAAMKDPRVLALALVYSGTSTGLYAIGIWAP
                     MIISHFGFSYFELGLVNAIPNVVAVVGMVLWAKNSDRTGERRLHVSIACLAAGAGMLL
                     AGYAPSALLLIIGLSVANFGINAAKPPLWSMPTQFLSGSAAAAGIALINAMGSLIGGT
                     IGPMVIGKLREVSGDYSLGLYFVSGTLIVSAVISWMFGRGQTKSSVTPTVS"
     gene            6711..8045
                     /locus_tag="VHV99_21300"
     CDS             6711..8045
                     /locus_tag="VHV99_21300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX3637970.1"
                     /translation="MSHSFPSPSIREIRDAIRAGTRTVDQTVQQAFEAAQRLQPELQA
                     FIHLPASPIVNRAAPHAPLAGVPVGVKDLIDTADMPTAYGSLAYEGRRPGKDAWVVAR
                     LREAGATILGKTVTTEFAWRQPGATTNPWNSDHTPGGSSSGSAAAVAAGIVPLALGTQ
                     TFGSVIRPAAYCGVVGVKPSYGAIARTGVLPLSGSLDHLGVFTTNVADAVHALSILAG
                     SDADDPHTSHNSRQGKNPGNAAKRPRRIGVLREQIGGPIDKAQQHVLNQLALRMRADG
                     AEIVGADLPAELLDAARHASILLAVEAALTHGELVDRSPALVSAPMKALVEEGRALPA
                     TEYAKAKVLQVKMARRFDRWLTEAAGLDALLVAPAAGEAPGGLDYTGDAAFCTPWTFL
                     GVPAVTLPVGFGPGGLPLGVQLVGASQHDAALLSLAEWVEGRTGWNIAVASR"
     gene            8141..9760
                     /locus_tag="VHV99_21305"
     CDS             8141..9760
                     /locus_tag="VHV99_21305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013343405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HEX3637971.1"
                     /translation="MSAEFLNPVRGIRGMAVTPHALASQSALAILRDGGNAVEAMIAA
                     AATIAAVYPHMNSIGGDGFWLISRPGEEPVGIEACGAAAAAATIDAYRAHGLQSIPFR
                     GPLAANTVAGTVSGWSLAHRWSTDTLGGKLPLTRLLEDAIAYAGRGIPVTRSQSECIA
                     LKRNELESVAGFAETFLTSDNPLGVPAVGDRFVQPRLAATLERLAHAGLDDFYRGDLA
                     RSIAADLRALDSPLSLADLEQHHARLRTPLALTHSLGTIYNMPPPTQGLVSLQILGVL
                     DRVLRADMDPLGPEYVHACVEATKLAFAIRDKHITDPDYMDVDPQALLAPSVLDAMAE
                     KISMSAAAPWGRGLGPADTVWLGVIDADGVAVSFIQSIYHEFGSGIVLADSGINWQNR
                     GCSFSLDASARNPLTPGRRPFHTLNPALARFADGRTMVYGNMGGDGQPQSQSAVFSRI
                     AHFGWNPQAAIDAPRWLLGRTWGQTNESLKLEARFPVDTFNTLRRLGHDVEQMRPYDE
                     AMGHAGAIVRHSNGCLEAGFDPRSDGAAAGW"
     gene            9921..11183
                     /gene="oxlT"
                     /locus_tag="VHV99_21310"
                     /pseudo
     CDS             9921..11183
                     /gene="oxlT"
                     /locus_tag="VHV99_21310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013343404.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate/formate MFS antiporter"
BASE COUNT         2027 a         3541 c         3471 g         2179 t
ORIGIN      
        1 gcgcctgcaa caggctgatc tcgatccacc gtgctacatt aattgcaact tgcaacaatt
       61 gaaattcaca cttttacagg cacgaagcgt gccataattc gggaaatcca gtacgtgtgg
      121 gcaagtcggg cgattgcccc accggcaagc acgcgctgca gtgcgcggga gttgtgcaag
      181 tgcgcgcgag gacggtgcat cacacagtac aagatcgtcg tcgcaccgat tccgggcgag
      241 agagcggcgt gactcatcgg tagcgatttt ttttggacgc tatttaaaat acgacttaag
      301 tacctatttt tcacacacgc gaaaggaacg tttcgatgga ctatccgagg atcgcagtcg
      361 gggcattagg gggcaccata tgcatgaccg ccgacaatgc cgatgctggc gcgttgccac
      421 gtctttccgc gcaacaactg gtgactgcgg tcccaggtct ggctgatatc gcgcagattc
      481 acgccaagac gcttttgcaa ttacccagcc cttcgctgaa ctttgcgcat gtgctggaca
      541 gtctggcgtg ggcagagcgc atggtggctg ccggcgccgc cgggatcgtg ctgacccagg
      601 ggaccgacac gcttgaggaa accgccttcc tgctcgatct tctgtggacg catccacagc
      661 cgatggtggt cacaggcgcg atgcgcacgc cgctggccgc aggcgcggac gggcccgcca
      721 atcttcttgc cgcggtggcc tgcgccgcgc acggcgccag tcgcgggcgc ggtgtagccg
      781 tagtcatgaa caacacgatc catgaggcgc gctgggtgcg caaagcgaac tcgatgtccg
      841 tcgaaacctt cgagtcgccg gtcgtggggc cagccggcat ggtgcttgaa ggtatgccgc
      901 agtatttccg cccaccgtca aggcgttatt ccccgcgctt cgcaaagccc gacctgtcgg
      961 ttcgtgtggc gctcgtcgag acctgtctgg gcgagacggg tcagatgctc gacgcggtcg
     1021 tccacgcggg gtatcacggg atcgtcatcg cggcctacgg cgcaggccat gtttcggatg
     1081 acgaagcaca gcgcatcgag gcgctggcgc cgcagctgcc gatcgtgatc tccagccgca
     1141 cgggatcggg atcgacggcc gcgcgcacct atggattttc cggctcggaa atcgacctgg
     1201 cccgccgtgg cgctttgttt tcggggtggc tcgggccgcg caaggcgcgc ctgcttctct
     1261 ggctgctgct cgccacgggc gcgccgcgca acgtatgcgc cactgcgttc gaattctgga
     1321 agcgcgtcga caatgccgac tagacgcccg gcacaatgtg cattgccagc cggctcgacc
     1381 gaggtgttcc gggttgcggt gcagatcgca gttgagcgag aaatcagcgg cttttttgcg
     1441 atttcagccc gctcccggcc ccttcccgag aaagccttcg agaaactcga tgaacgccgt
     1501 caccttcgcc gtcagattga gccgctcggg atacacggcg tagatatctg ccggcggcaa
     1561 cgaccaatcg ggcagcaact gcttgagacg gccagattgc agataggagg tcgcatccca
     1621 ctccgaacgc atcaggatgc cctgtccgtc aagtgcccag acgagcgtcg cttcgccatc
     1681 attgctgctc agcacaccgt gcaccttgac cgtctcgtgg cgcttgccct tgctgaaatg
     1741 ccaggtgccg taggccgtgt cgttttccct gagtacgatg cattgatgcc gctgcagatc
     1801 gcgcggcgtg tcaggctcgc ctttcgcgcg cacgtagatc ggcgacgcgc agaccatccg
     1861 gcggttcgtc gcgaccttgc gtgcgatcaa cctcgaatca ggaatctcgc cgaaccggac
     1921 gccgacgtca aacgccgctt cggcgagatt caacggccga tccgtcaact caagctgtac
     1981 ctcgacgtcg ggatactgcc gatgaaacgc tgcgatggcc ggggcgatgt gacggcggcc
     2041 aaacccgaac gtcgcattga cgcgcagcag cccctttggc gtcgctctgc tgctcgaaat
     2101 ggtccggtcg agttcctgca atgcagacag cagcttcgac cccgtcgtca ggtattgctc
     2161 gccctcatgc gtcatgctga gccgccgcgt ggtccggttc agcagccgca caccgaggcg
     2221 ccgctcaatc tgcgcaaggc gcgcgctcac cgctggcggc gtgaccccaa cgtcccgcgc
     2281 gagcgccgag aggctgccat gtttgacgag ttcgacgaaa aactcgaggt ccgaaaccgc
     2341 atccattgat aaatataatt taagagtagt ttaaattcat ctgaattcta tatcagaata
     2401 gccagattag actcctgtcc atcggtcgat gatgaccgcg ccccacgcct cagattcaac
     2461 cggccagagg agtccatgcc gcacaccttc tatcaaaagc tcgtcgcatc ccatacagtc
     2521 gaccgcctcg acgagcagca tgtgctgctg tatgcggatc tgcatatcat gaacgagtac
     2581 acgagtccgc aggccttcgc cgggctcgct gagaaagacg tcctcgtgcg ccggccgcgg
     2641 cagcagatgg gcattgtcga ccacgtcatc ccgacgcgcg cggtgccggt tgccgcccgc
     2701 acgatcgaca tcgtcgacgc atcgaagcaa gccgcgaatt tcacacgcaa ctgcgtcacg
     2761 caaggcattc acctgttcga cgtgaccgac ccggcgcaag ggatcgagca cgtcgtcgca
     2821 ccggaaatcg gactgatccg gcccggcatg gtcgtactgt gcggcgacag ccacacgacg
     2881 acctacggtg cgctcggcgc actcggcttc gggatcggca cttccgaagt cgaacacgta
     2941 ctcgcgacgc aaacgctcgt ctatcgcgtg gcggacgaca tgcggatcac cgtcgacgga
     3001 gaactgccgg ctggcaccac gtcgaaagac ctggtgctgc acatcattgc ccgtatcggc
     3061 gcacagggcg cccgcggctt cgcgctcgaa tatgcgggat cgacgatcgc ttcgctttct
     3121 gccgaggcgc gaatgaccct gtgcaacatg acggttgaag ccggcgcgcg cgctgcgttg
     3181 attgcaccag acgacacgac gatcgactac gtgcgggccc gcgtgcgcga tctggatgca
     3241 gcgacactcg atagtgcaac ggccgcatgg cgctcgctgg gctcggactc cgatgccgtc
     3301 ttcgatgtcg aatatcatgt cgatgcatcg caaatcgcgc cgtatgttac gtggggcacg
     3361 agtccggacc aggcgattcc cgtcgacgga cacattcccg aggtcgacac ggcaaccggc
     3421 atcgacgcgg caaccgcgcg caaggcactc gactacatcg cgctgcccgc cggcgcaagc
     3481 atcgagggca cgccaatcga tcgtgtgttc attggctcgt gcacgaatgc gcgcattgaa
     3541 gacctgcgag cggcagcggc catggtacgc ggacgcaagg tcgcggcatc ggtgcgcgcg
     3601 atggtcgttc ctggttccgg catggtccgc gaccaggccg agcgcgaagg cgtcgcgcag
     3661 atcctgatcg acgcgggctt cgaatggcgc cagcccggct gttctatgtg cctcgcgatg
     3721 aacgacgacg tcctggcacc gggcgagcgc tgcgcgtcga cgaccaatcg caacttcgaa
     3781 ggccgtcagg gccgcggcag caagacccac ctgatgagtc ccgccatggc cgccgccgcc
     3841 gcgatcgcgg gccatatcgt cgacattcgc aaggagcttg tacatggctg atcgccgtct
     3901 cattcagggc accgcagcac cgttgccgat caacaatctc gataccgacc agatcatgcc
     3961 gaagcagttc ctgcttggca tcgacaagtc ggggcttgcg cgtgggctgc tttacgacct
     4021 gcgttacgat gagcgcggtc aggcgcgcga gacgttcgtg ctgaatcgcc cggaatatcg
     4081 cgacacacgc attctgatcg gcggctcgaa tttcggctgc gggtcgagcc gcgaacacgc
     4141 cgtgtgggga ctgcaacaag ccggcatcga agcggtcatc gcgccgagct acggcgagat
     4201 cttctacttc aacgccggca acaatcgcct gctgctcgtg acgctcgcgc cggaaatagt
     4261 cgatgcactc gttgccgaac tggctggtgc ggacaccaag caactgtcga tcgacgtcga
     4321 aaacgaagtt gtcgtgaccg cctcgggcga gcgccatccg ttctcgatcc cgtcgcggca
     4381 aaaacgcatg ttcatggacg gtctcgacat ggtccagatg acacttcagc aatccgatca
     4441 gatcgacgcg tttgaaaagc ggcattgggc agccaatccg tgggcacgca tcgatcttcc
     4501 cgcccagcaa cgcagcactt tgtaactaga cgcgcacacg ccttccacgc gtgtgcgtca
     4561 gcatcgagcg acacgaattt caacctgata acgagaccgg cgcgggaccg cgccggcggg
     4621 agacaaccat gccccagcct tccgaacccg ctaacgtgcc ggacgcgcac gcgctagaag
     4681 cccgcacagt caagaagact acatggcgca tcgtgccatt catcatcgcg ctgtacttcg
     4741 tgagcttcct gaaccgcgtc aacgtcggct tcgccggcct gacgatgaat cacgacatcg
     4801 gactcacgcc ggcaatgttc ggcttcggcg cagggatctt ctttgtcggc tacattgtgt
     4861 tcggcatgcc gtcgaatctc gcgctgcagc gcgtcggcgc gcggcggtgg attgcgttgc
     4921 ttatggtcgt gtggggggcc ctctcggcgg cgaccgcgtg cgtcagcggt ccctatagct
     4981 tctatgtgct gcgcttcctg ctagggctgg cagaggccgg gttcttcccc ggcatcatcc
     5041 tgtacctcag ctattggttc ccggcccggc atcgggcgct tgtgacggcg atcttcatga
     5101 ccgccgcgcc gttgtcgaac atgatcggct cgccgatctc cggcgccatc atgggcatgg
     5161 acggcatcgc acaaattcat ggctggcagt ggctcttcat tctcgaagcg gcgccgacca
     5221 ttgcgctcgg cgtgatcgcg tacttcttcc tgaccgacaa gcctgaagat gcgcgctggc
     5281 tcgctaccga cgaacgtgag tggctcacgc tcgagatgga ccgggagcgt tccctcaagg
     5341 caacgcacgc cgcatccagc gtatgggcgg ccatgaagga tccgcgcgtg ctcgccctcg
     5401 ccctcgttta ttcgggcacg tcgaccggtc tgtacgcgat cggcatctgg gcgccgatga
     5461 tcattagtca cttcggcttc agctatttcg aactagggct cgtcaacgca attcccaatg
     5521 tcgtcgccgt cgtcggcatg gtgctctggg cgaaaaactc cgaccgcacc ggcgaacgca
     5581 gattgcacgt gtcgatcgca tgtctggccg caggcgcagg gatgctgctc gccgggtatg
     5641 caccgtccgc actgctcctg atcatcggac tgtcggtcgc gaattttggc atcaacgctg
     5701 cgaagccgcc gctgtggagc atgcctacgc agtttctctc cggttcggct gcggcagcgg
     5761 gcatcgcatt gatcaacgcg atgggcagtc tgatcggcgg gacgatcggc ccgatggtca
     5821 tcggcaagtt acgcgaagtc agtggcgact actcgctcgg actctacttc gttagcggca
     5881 cgctgatcgt gtccgcggtc atttcctgga tgttcggccg tggccaaacg aagtcgtccg
     5941 ttacacccac agtctcctga ttctgtcgtg cgaagctgcg cccgattttt cagcacttgg
     6001 atcagtgcgc aaaagtcaca caaaacctcg ccaggacgat aacgacgcaa acgcggttgc
     6061 ctaaccgcta acgcgtcgtc gaagctcctg acacagatga atgtagcggc ctttgccaca
     6121 gatcacgggg cagaagcgcc gctatccgga aagcactcgc tggcgtcggg gagaagtcgt
     6181 ttttcggctg cccccgacgc cacggcattt gtgcggcggc cgaagcgaca cttcgtgccg
     6241 catgccgcta gccaaaatgc cgactagatt gaattggcaa cgcattgtat cgagcgcccc
     6301 tctcgccgca acatagacgt tctgatgcgt tcgcgttggc accgcactta aattctggag
     6361 gcggtgctcg caccgatttt caatcctaaa tgtgttgcaa cgggctttcg ccggcgtttc
     6421 gcccagcggc ggtgcaccgc caattttgtc gtgccggatg caccgttttc gcgcgtatct
     6481 gcacactttc cgaacatggc agtgtgctta cagccagctg aacgcactcg attcgcactt
     6541 gtataagggc cttcacggga tggcacgctt cgtgcacatc gaatcaatgc gaatatttca
     6601 attgttgcaa tttgcatcta tatttcgcgg acaaccgacg tgcagcgtga cctgctgttc
     6661 cttcggcgca ccgtattgca gaaccttatt caccaggcac ttgccgcgaa atgagtcatt
     6721 cttttccttc cccgagcatc cgcgaaatcc gtgacgcgat ccgcgcggga acgcgtaccg
     6781 tcgatcagac tgtgcaacag gcattcgagg cggcacaacg gctgcagcct gagttgcaag
     6841 cgttcatcca tctgccggcg tcgccgatag tcaatcgtgc cgctccccac gctccgctcg
     6901 cgggcgtccc tgtcggcgta aaagacctga tcgatacggc cgatatgccc accgcgtacg
     6961 gatcgctggc ctatgaaggg cgcaggcccg gcaaggacgc atgggtggtc gcccgtttgc
     7021 gtgaagccgg tgccacgata ctcggcaaaa cggtcaccac cgagttcgcc tggcgtcagc
     7081 ccggcgccac gaccaacccg tggaatagcg atcacacgcc tggcggctcg tcgagcggct
     7141 cggctgctgc cgttgcggca ggcatcgtgc cgctcgcgct cggcacgcag acgttcggat
     7201 ccgtcatccg tcccgccgcg tattgcggtg tggtcggggt aaagccgagt tacggcgcga
     7261 ttgcgcggac tggcgtgttg ccgttgtccg ggtcgctcga tcaccttggc gtgttcacca
     7321 ccaatgtcgc tgacgcagtt catgcgctat ccattctggc gggtagcgat gcggacgatc
     7381 cacacacctc gcataacagt cgccagggga aaaacccggg caatgcggca aagcggccgc
     7441 gacgcatcgg cgtgttgcgc gagcagatcg gcggaccgat cgacaaggcg cagcagcacg
     7501 tactgaatca acttgccttg cgtatgcgcg ctgacggtgc ggaaatcgtc ggtgccgact
     7561 tgcctgccga actgctcgac gctgcacgac atgcttcgat cctgctcgca gttgaagcgg
     7621 cgctgacgca tggcgaattg gtagaccgtt cgccggctct tgtcagcgca ccgatgaagg
     7681 ctctggtcga ggaaggccgg gctttgcccg cgactgagta tgcgaaagcg aaggtattgc
     7741 aggtaaagat ggcgcgtcgc ttcgaccggt ggctcaccga agcggcggga ctggacgcgc
     7801 tgctggtagc gcccgcagcg ggcgaggcgc ccggcgggct cgactatacg ggcgatgcgg
     7861 cgttctgcac gccctggacc ttcctcggcg tgccggcggt gacgttaccg gtcggcttcg
     7921 gtccgggagg gttgccgctt ggcgtgcaac tggtcggggc aagtcagcac gatgccgcgt
     7981 tgctgtcgct tgccgagtgg gtagaaggta ggaccggctg gaacatcgca gttgcttcgc
     8041 gttgatgccg gcttcgttgt gtgcaggaag taacacgtga tcatgcgaac ccgcagcggc
     8101 tgagcgggga gcgattgatc aattgattgt ttgaggaaac atgtcagcag aatttttgaa
     8161 tccggtgcgg ggtatccgcg gaatggccgt cacaccccat gcactggcgt cgcaaagtgc
     8221 gctcgcgatc ctgcgcgacg gcggcaatgc cgtcgaggcg atgattgccg ccgcggcgac
     8281 gatcgcagcg gtgtatccgc atatgaacag catcggcggc gacggcttct ggttgatctc
     8341 gcggccgggc gaagagcccg tcggcatcga ggcgtgcggt gcggcggcag cggcggcgac
     8401 gatcgatgcc taccgcgcac acggcctgca atccatcccg tttcgcggcc cgctggcggc
     8461 gaatacggtg gccggaaccg tgtcgggctg gtcgctggcg catcgctggt cgactgatac
     8521 gctcggcggc aagctcccgc tcacgcgcct gctggaagac gcaatcgcct acgcaggacg
     8581 cggtattccg gtgacgcgca gtcagtccga atgcatcgcg ctcaagcgga acgaactcga
     8641 atcagtcgcg gggttcgccg aaacgtttct gacgagcgac aacccgctgg gcgtgccggc
     8701 cgtcggcgac cgctttgtgc agccgcgcct tgccgcaacg ctcgaacgtc tcgctcacgc
     8761 cggtctcgac gacttctacc ggggcgacct cgcacgcagc atcgcggccg acctgcgtgc
     8821 actcgatagt ccactgtcgc tagcggacct cgaacagcat cacgcaaggc tgcgcacgcc
     8881 gctggcactc acgcattcgc tcggcacgat ctacaacatg ccgccgccca cgcaagggct
     8941 ggtatcgctg cagattctcg gggtgctcga ccgtgtcctg cgcgccgata tggatccgct
     9001 cgggcccgag tacgtccacg cgtgcgtgga ggcgaccaag ctcgcgttcg ccatccgcga
     9061 caagcacatc accgatccgg attacatgga cgtcgatccg caggcgttgc ttgcgccgtc
     9121 tgtgctcgac gcgatggccg aaaagatttc aatgtccgcg gcagcgccgt ggggtcgggg
     9181 tctcggaccg gccgacaccg tgtggctcgg cgtgatcgac gccgatggcg tggccgtcag
     9241 cttcatccag agcatctatc acgagttcgg cagcggcatc gtgcttgcgg attcgggtat
     9301 caactggcaa aaccgcggct gcagcttctc gctcgatgcc tccgcgcgca atccactaac
     9361 gccaggacga cgtccgtttc acacgctgaa cccggcactc gcgcgcttcg ccgatggacg
     9421 cacgatggtg tacggaaaca tgggcggcga cggccagccg caatcgcaaa gcgccgtgtt
     9481 ttcgcggatt gcgcacttcg gctggaatcc gcaagccgct atcgatgcgc cccgctggct
     9541 gctgggtcgg acctgggggc aaaccaatga atcactcaag ctcgaggcgc ggtttcccgt
     9601 cgacacattc aatacgctgc gccgtctcgg ccatgacgtc gagcagatgc ggccgtacga
     9661 cgaagcaatg ggccacgccg gcgcgattgt ccggcacagc aacggatgtc tggaagcagg
     9721 tttcgacccg cgcagcgacg gcgccgcggc cggctggtag ggccacctgc tgaacagggt
     9781 gcggccagtc cgcccgcacc ctgttgaatc gatgacacgc gggcgttcgc gccgacggta
     9841 aggacagcgt acgtccgttc atctgtcgac acatcaatcc aaaacggaga aaaaacacct
     9901 tgaacacccc cacgcaatca ttgaaaggtg ccgccccgcc cggcgcccaa accgccgcgc
     9961 atacgcgctg gatccagctc gggctcggcc ttgtttgcat gatgacgatt tcgagtccgc
    10021 aatatgtatg gactctgatg acgaaaccgc tcatggccaa gctcggcatc gcgctgccgg
    10081 aactgcaggt gacattctcc ctgctggtgc tgctgcaggc gtttttctca ccgttccagg
    10141 gcgcgctgat cgatcgtttc gggccgcgca tgctgatctc gatcggtacg ctgctgtcag
    10201 gcgtgagctg ggtgttggcc tcgtatgcgc acagcacgtc gatgctgtat ctcacgtatg
    10261 gctgcatcgg cggtctcggt accggcatcg tctatgtcgg cgtggtggga ttcgtggttc
    10321 gctggtttcc cgatcggcgc ggtttcgccg ccggggtagc ggccgcgggc tatggcatgg
    10381 gcgccattgt cacgaccttt ccgatctccg catcgctggc aacgcacggc gtcgaatcca
    10441 cgctgtggct gtacggcatc atcttcgcgg cggtcggcct gctcgcctcg cagggtctgc
    10501 gagtaccgcc cgcggccgac aggatcggct cgacagtatc ccgcacagca gtcacgacgc
    10561 gcgattaccg cccgtccgag atgctcaaga cgccgctgtt ctggctgatg ttcctgatga
    10621 tgacgatgat gtctacgtcg ggcctcatgg tcacgtcgca gatggcaagc ttcgccgcgg
    10681 acttcggcgt cagcaagatc ctggtcttcg ggctcgccgc gctgccgctg gcattgacga
    10741 tcgaccgctt cacgaacggt ctgacccgtc cgctgttcgg ctggatatcg gatcgcctgg
    10801 ggcgcgaaaa cacgatgtgt ttcgcgttcg cgctggaagg tgtggcgatg gcgctctggc
    10861 tcatgacgcg cgacaacgcg gtgctgttcg tactgctgtc cggcgtggtg ttcttcggct
    10921 ggggtgaaat tttttcgctg tttccttcaa ccctgaccga cacgttcggt acccgttatg
    10981 gcaccacgaa ctaaggctgg ctttacatct cgttcgggat tggctcgatc ttcggcggcc
    11041 cgctggccgc gttgctgcat cagcacaccg gcagctgggt gccggtgttc ggctgcggga
    11101 ttgcgctcga tttgactacc gccgcgcttg cactgttcat gttgaagccg tcgcgcgcac
    11161 gttttgtcag gacgcgagga taacgagggt tgtgtcgtag tatggcggct caggcgaa
//
LOCUS       DATASH010000795         6416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2704908,
            whole genome shotgun sequence.
ACCESSION   DATASH010000795 DATASH010000000
VERSION     DATASH010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6416
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2704908"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VHV99_21315"
     CDS             <1..376
                     /locus_tag="VHV99_21315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX3637972.1"
                     /translation="GHRDKAIGESLLLLLSLKGFDAVYAADVIKLDRFLKWWKPHAIL
                     LDTRLDSASDYQFTRAVREEPSNAGILLLAMSNIWPLDPIAGLKQAGFDGHCRRPCSL
                     WRVVDILEGYFAQPNVGPAGPH"
     gene            complement(511..1050)
                     /locus_tag="VHV99_21320"
     CDS             complement(511..1050)
                     /locus_tag="VHV99_21320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX3637973.1"
                     /translation="MHSHELVKQLDIIPAEQLSAHLPAHVVGHLPAQGVTVFAVSDDA
                     SDTAAFSERYGFGLEDCANTIVVRYKKEGAEHYAALVSLGSLRLDINGAVKAALGAQR
                     LSFARREAAVEHSGMEFGGITAFGLPADWRILVDAAVLERAQIVMGAGVRAAKLLLAP
                     AVLQQWPRCEVASLTLPAE"
     gene            1244..2572
                     /locus_tag="VHV99_21325"
     CDS             1244..2572
                     /locus_tag="VHV99_21325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX3637974.1"
                     /translation="MDIDTLSAENLQLVARKQANWAISAFKQFADDRCAAMAASIAFY
                     AAFSLAPTLVMVIAVAGWFFGAEAARGELFTHIHGLLGDQAAAGVQTIVENAHHSGSA
                     GGIAAIISFTMLAIGASATFSSLNSALNIVWPYTGRRSSSVIAMVRVRLISFGLVLGV
                     AFLLIVSLVLDTVITFVGKWLWGNSPYVVIGNLLQLGVGLLVLAFAFAGLLKFLPDDK
                     VRWRDAFVGGIVAAVLFSAGKKLFALYIAHAGMASSFGAAGSLAVLLMWLYFSAAVLL
                     LGAEFSAARGRMHDPRGGWGIQEESPPGSRAKLASVLAASTVSADALLREDDRDARAA
                     ANFAPATGAAAATAPGAATPASTLRRTQARNKASARATALKIGRAVVLTEAQATRLAA
                     VTLVEAGRTAVAADRYVRRHPWTSVLFAAAAGLAAATIARRNEDENGRRG"
     gene            2910..4094
                     /locus_tag="VHV99_21330"
     CDS             2910..4094
                     /locus_tag="VHV99_21330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX3637975.1"
                     /translation="MKRIALSTLSLALLGVTGVAHAQSSVTLYGLIDESVQFAHNTVD
                     ASGKNSNQVGLVGGNLQGDRWGLKGTEDLGGGLKAIFQLENGFDVNSGKLGQSGRMFG
                     RQAYVGLTGDQWGTVTLGRQYDPLVDLVQPLTADNYFGSTFTTPGDVDNNDNTSRTNN
                     AVKYTSPVWGGFQFEGMYALGGVAGSTGSGQTWSGAATYATGPFSVAAGYFKADNGNT
                     AASRFTGTTPSWNGSTSDGTFDNQVSSFYASAKSIGIASVAAQYVAGPFTVNLRYSNA
                     LYKPDGLSGFGSTEKYNVGGVYLGYQATPAMLVGVGYIYTKATGDTSATYNQVSLGGD
                     YNLSKRTDIYLVGAWQHATGDQRNSATGGLTSAGAAIGSYSYNSASNDQALVSLGIRH
                     KF"
     gene            complement(4394..5266)
                     /locus_tag="VHV99_21335"
     CDS             complement(4394..5266)
                     /locus_tag="VHV99_21335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome-type protease"
                     /protein_id="HEX3637976.1"
                     /translation="MTYCVAMSVDEGLVFLSDTRTNAGVDHISTARKMSVFEHPGERM
                     LVLLGAGNLSLTQAVLHELSEPTDPAQPTLWSAPTMADAARVIGRAVRSVHQREAEAL
                     QEFGVDFNCSFILGGQIAGNRPRLFMIYAAGNFIEASAVNPYFQIGEAKYGKPIIDRV
                     LTPSTPLDEAAKCALISMDSTLRSNLSVGLPLDLLVYQKDSLRITRFVSIDHDNAYFE
                     MIHRTWGERLRQVFGEIPDPDWQDSPNVPVLQRERALVLHRAPVGADGVEHELDAKPA
                     QTLAQSEKGKAQRR"
     gene            complement(5363..6190)
                     /locus_tag="VHV99_21340"
     CDS             complement(5363..6190)
                     /locus_tag="VHV99_21340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEX3637977.1"
                     /translation="MYLTIRHDTSYRYEATVHYSIQQLRLTPSSGASQVVRRWSIDAP
                     GKLDATFDAYGNVLHTLVINKPHDEIRLHVAGEVDTIPLLDGQLPDAVGPIPLEHFTC
                     STRLTEADAAVRELAASVPSLASSSNLIALSEQILQRVKYNPGITEVTSTAAQALALG
                     NGVCQDHAHLMLACCRARGVPARYVSGYIEPGEVLSEGSPLGGEHGASHAWVDVWLDG
                     KGWISIDVTHATFASEIYCRLAVARDYEAAAPVRGRRIGGMDEQLKVSVTVSAQLPQ"
     gene            complement(6203..>6416)
                     /locus_tag="VHV99_21345"
     CDS             complement(6203..>6416)
                     /locus_tag="VHV99_21345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HEX3637978.1"
                     /translation="EGVCANLAMLRTSGSNQCERFAGKLRAELVYSDIRQIFEAGLHA
                     YLTQFLARVFELGNLVARTYLMLPVA"
BASE COUNT         1214 a         2021 c         2001 g         1180 t
ORIGIN      
        1 aggacatcgc gacaaggcga tcggcgaatc gctgttgctg ttgttgtcgc tgaagggatt
       61 cgacgcggtc tatgccgccg acgtgatcaa actggatcgc tttctgaaat ggtggaagcc
      121 gcacgcgatc ttgctggaca ccagactcga ttccgcctcg gactatcaat ttacgcgcgc
      181 cgtgcgcgag gagccatcga atgccggaat actgttactg gcaatgagca acatctggcc
      241 gctcgatccg attgccggac tcaaacaagc cggattcgac ggccattgcc ggcgaccgtg
      301 ttcgctctgg cgcgtggtcg atatcctcga aggctatttc gcccagccca acgtggggcc
      361 ggccggtccg cactgaacac ggcggggcgg atcactgccg ttgcggcagc gcgaacccgg
      421 caatggccgc cgccgattgc gcggtctgcg ccgcgccgcc ggcgttcccg gcgctctgct
      481 tcacggccgc gctacgctgt cccacgctgc tcactcggcc ggcagcgtaa gcgaggccac
      541 ttcgcagcgc ggccactgct gcagcaccgc gggcgccaac agtaacttgg cggcccgcac
      601 cccggctccc atcacgatct gagcacgttc cagcaccgcg gcatcgacga gaatgcgcca
      661 atctgccggc aagccgaagg cggtaatgcc gccgaactcc atcccgctat gctccacggc
      721 ggcctcccgc cgggcgaacg aaaggcgctg cgcgccgagc gcagccttca ccgcgccgtt
      781 gatgtcgagg cgcagcgaac ccaatgaaac cagcgccgcg taatgctcgg cgccctcctt
      841 cttgtagcgg accacaatcg tattggcgca gtcttcgagg ccgaagccgt agcgctcgct
      901 gaatgcggcg gtgtccgaag cgtcgtcgct gacggcaaat acggtgacgc cctgggccgg
      961 caaatgcccg acgacatgcg ccggcaaatg cgcgctcaat tgctccgcgg gaatgatgtc
     1021 cagctgcttg acgagttcgt gcgagtgcat ggtagaaaag agtgtgaagg tccagcgaca
     1081 ttctaacggc atgcctgcgg gtgccgcatc gcgggcatgc gggtgccgcg cttgttatag
     1141 tgacaggcac aaggtttgcg aaaggtatcg gtgcgaggac cgcctcgcca cctcgacggc
     1201 acgcacacgg catccgggcg caagtatccc attgagaacg accatggaca tcgacacgct
     1261 ttccgccgag aacctccagt tggtggcccg caagcaggcc aattgggcaa tcagcgcatt
     1321 caaacagttt gccgacgacc gctgcgcggc catggccgcc agcattgcct tctacgcggc
     1381 attctcgctc gcgcccacgc tggtcatggt gattgccgtg gcgggctggt tcttcggcgc
     1441 cgaagccgcg cgcggcgagc tgttcactca cattcacggc ctgcttggcg accaggccgc
     1501 cgccggcgtg cagaccatcg tcgagaacgc ccatcacagc ggcagcgccg gtggcatcgc
     1561 ggcgatcatt tcattcacga tgctggcgat cggcgcttcg gccaccttct cctcgctcaa
     1621 cagcgcactc aatatagtgt ggccgtatac cgggcggcgc tcgtcgagcg tgatcgcgat
     1681 ggtgcgcgtg cgcctgattt cgtttggcct cgtgctcggg gtcgctttcc tgctgatcgt
     1741 ctcgctggtg ctcgatacgg tcatcacgtt cgtcggcaag tggctgtggg ggaactcgcc
     1801 ctacgtggtg atcggcaacc tgctgcaact cggagtcggc ttgctggtgc tggccttcgc
     1861 ctttgccggt ctgctgaagt ttctgccgga cgacaaggtg cggtggcgcg atgcgtttgt
     1921 cggcgggatc gtcgcggcgg tgttgttttc ggcgggcaag aaactcttcg cgctctatat
     1981 cgcgcatgcc gggatggcga gctcgttcgg cgcggccggc tcgctggccg tgttgttgat
     2041 gtggctgtac ttctcggctg ccgtgcttct gctcggcgcg gagttttcgg cggcccgcgg
     2101 acgcatgcac gacccgcgcg gcggctgggg catccaggaa gagtcgccgc ccggcagccg
     2161 tgccaaactc gcttcggtac tggcggcctc gacggtatcg gccgatgcac tgcttcgcga
     2221 ggacgaccgc gacgcccgtg cggctgccaa ctttgctcct gcaactggag cggcagccgc
     2281 cacggcgcct ggcgcggcca ctcccgcgtc gaccttgcgg cgcacgcaag cgcgcaataa
     2341 ggcttcagcg cgggcgaccg cgctcaagat cggccgcgcc gttgtcctca ctgaagcaca
     2401 ggcgacacgt ctcgccgccg tcacgctggt ggaagccggc cgcacggccg tcgcggctga
     2461 tcgctatgtc agacggcatc cgtggacctc ggtgctgttt gcagcggcgg ccggcctggc
     2521 tgccgccacc attgcgcgcc gcaatgagga cgagaacggc cgtcgcggct gaggaccggg
     2581 acgccacgtc aagcgaaacg tgaccccact tttccatgga tgctgcagaa cgggtcaatg
     2641 cagttagacg actagattac aaaaatcatt caaacacgtg tctaaattga aggcactcga
     2701 accttcactg tcagcaaaac aacaataatg cgtcgtcaac gaatcaatat tgcgaaaacg
     2761 gcaataatcg ttaatggtct ttaaatacaa ggctttgcgt cgtttgtcac tttttagaga
     2821 cactcttttt tttctgttct ggtcgattga ggcagtgagc tattctcctt tccgcaacaa
     2881 cgaaactaat tatttgcgtg gagatatgga tgaaacgaat cgcactgtct accctctcgc
     2941 tggcgcttct cggcgtcacc ggcgtggcac atgctcaaag cagcgttacc ctgtacggct
     3001 tgatcgacga atcggtccag ttcgctcaca acacggttga cgcgtccggc aagaacagca
     3061 accaggttgg cctcgtcggc ggcaacctgc aaggcgatcg ctggggcttg aagggtacgg
     3121 aagatctggg cggcggcctg aaggcgattt tccaattgga aaacggcttt gacgtgaaca
     3181 gcggcaagct gggtcaaagc ggcaggatgt tcggtcgtca agcctacgtc ggcctgacgg
     3241 gcgaccaatg gggtacggtc acgctgggtc gtcagtatga cccgctggtc gacctggttc
     3301 agccgctgac ggctgacaac tacttcggtt ccaccttcac tacgccgggc gacgtcgaca
     3361 acaacgacaa cacctcgcgc acgaacaacg ccgtcaagta cacctcgccg gtgtggggcg
     3421 gcttccagtt cgaaggcatg tacgctctag gcggcgtggc tggctcgacg ggttcgggtc
     3481 aaacgtggtc gggcgctgca acgtacgcaa cgggtccgtt cagcgtggct gcaggctact
     3541 tcaaagccga caacggcaac acggcagcca gccgcttcac gggaacaacg ccgagctgga
     3601 acggcagcac gtcggacggc acgttcgaca accaggtcag cagcttctac gcatcggcga
     3661 agtcgattgg tatcgcttcg gtcgctgcgc aatacgtggc cggcccgttc acggtcaacc
     3721 tgcgctacag caacgcgctg tacaagccgg atggactgtc gggcttcggt tcgaccgaga
     3781 agtacaacgt cggcggtgtg tatcttggct accaggcaac cccggctatg ctggtgggcg
     3841 tgggctacat ctacacgaag gctacgggcg acacgagcgc aacgtataac caggtttccc
     3901 tgggcggcga ctacaacctg tcgaagcgca cggacatcta cctggtcggc gcatggcaac
     3961 acgcaaccgg cgaccaacgc aattcggcga ccggcggcct gacctctgct ggtgcagcga
     4021 tcggttcgta cagctataac tcggcttcca acgaccaagc attggtcagc ttgggcatcc
     4081 gccacaagtt ctaaccgaac ttgatggact gctgaagcaa cacgcagttg aagaccagcc
     4141 acgggcgcaa gccggtggct ggtttttttt cgtttaccgg ttggcgccgg cgtgcgcggc
     4201 gagccgttga ctcgtgacac gtgcgtatca cgctgcgaat agatggaccg ccacgcgcgc
     4261 actgcgcgtc cgtcgtcatt gacccgacag gatcaaccgc ggggaagtaa aagaaaaaag
     4321 ccgccggtaa gccattgtca ggctcacgac gacctcgctg tgaagcttcg gaccgcaagc
     4381 ggccctgaat tgcctagcgc cgctgggcct tgcccttttc cgattgcgcc aacgtctgcg
     4441 ccggcttcgc atcgagctca tgctcgacac cgtcggcgcc caccggcgcg cgatgcagca
     4501 cgagcgcccg ttcgcgctgc agcaccggca cattgggcga atcctgccag tccggatcgg
     4561 gaatctcgcc gaacacctga cgcagccgct cgccccacgt acggtgaatc atctcgaagt
     4621 acgcgttgtc gtgatcgatc gagacgaagc gcgtgatgcg cagcgaatcc ttctgataga
     4681 ccagcagatc gagcggcaaa ccgaccgaca gattcgagcg cagcgtcgaa tccatcgaga
     4741 tcagcgcgca cttggcggct tcgtcgagcg gcgtggaagg cgtgagcacg cggtcgatga
     4801 tcggtttgcc atacttggcc tcgccgatct ggaaatacgg attcacggcc gacgcttcga
     4861 tgaaattccc cgccgcatag atcatgaaca gacgcggccg gttgcccgcg atctggccgc
     4921 cgagaatgaa gctgcaattg aagtcgacgc cgaattcctg cagcgcctcg gcttcgcgct
     4981 gatgcacgct acgcaccgcg cggccgatca cgcgggccgc gtcggccatg gtgggcgcgc
     5041 tccagagcgt gggctgcgca ggatccgtcg gttcggaaag ctcgtgcagc acggcttgcg
     5101 tgagcgacag attgccggca cccagcagca cgagcatgcg ttcgcccgga tgctcgaaca
     5161 ccgacatctt gcgcgcggtg ctgatgtgat cgacacccgc attggtgcgc gtgtccgaga
     5221 ggaacacgag cccttcgtcc acggacatcg cgacgcagta agtcataacg aaaggtaccc
     5281 gcaaaaaacg ccagaacggc actattgtaa tgcggcgcgg atgcgccttt accgcatatt
     5341 atggccgctg gcagccttcg ctctattgcg gcaactgcgc actgaccgtg acggatacct
     5401 tcagctgctc gtccatcccg ccgatacgcc ggccgcgcac cggcgcggcg gcttcgtagt
     5461 cgcgcgccac ggcaaggcgg cagtagatct cgctggcaaa cgtggcgtgc gtgacgtcga
     5521 tggaaatcca gcccttgcca tcgagccaca cgtcgaccca tgcgtggctc gcgccatgct
     5581 ctcccccaag gggacttcct tcggagagaa cctcgccggg ttcgatgtag ccgctcacgt
     5641 accgtgccgg aacgccgcgt gcgcggcagc atgccagcat cagatgtgca tggtcctggc
     5701 atacgccgtt gccgagcgcg agcgcctggg cggccgtgct ggtcacttcg gtgatgcccg
     5761 ggttgtactt cacgcgctgc agaatctgtt cggacaacgc gatcagattg ctcgagctcg
     5821 cgaggctcgg caccgacgcg gccagctcgc gaaccgccgc atcagcctcg gtaagacgcg
     5881 tcgagcaggt gaaatgctcg agcggaatcg ggccgacagc gtcgggtaat tggccgtcga
     5941 gcagcggaat cgtgtccact tcacccgcca catgcagacg aatctcgtca tgcggcttgt
     6001 tgatgacgag cgtatgcagc acattgccgt aggcgtcgaa ggtcgcgtcg agcttgccgg
     6061 gcgcatcgat gctccagcgc cgcaccacct gcgaggcgcc gctcgagggc gtcagacgca
     6121 gttgctggat cgaataatgg acagtcgctt cgtaacgata ggacgtgtcg tggcggatcg
     6181 tcaggtacat aaaaaaactc cgtcaggcga ctggcagcat cagataggta cgcgcaacca
     6241 gattgccgag ctcgaacacg cgagcaagga actgcgtcag ataggcgtgc aggccggctt
     6301 cgaaaatctg ccggatgtcg gagtacacca gctcggcgcg cagcttgccg gcaaaccgtt
     6361 cgcactggtt cgagccggac gtgcgcagca tcgcgagatt cgcgcacacg ccttcg
//
LOCUS       DATASH010000796         9273 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5474656,
            whole genome shotgun sequence.
ACCESSION   DATASH010000796 DATASH010000000
VERSION     DATASH010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 9273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9273
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5474656"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            792..1895
                     /locus_tag="VHV99_21350"
     CDS             792..1895
                     /locus_tag="VHV99_21350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013343198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX3637979.1"
                     /translation="MVGKRFDRTMMASLAGVWVALAVSGCANVGQQGAQTGAVSGASG
                     ASAASSVPAAAAVPASDALAGTQVKLAADEGTPLKKAPPLTYRVKRGDTLARIAQHHH
                     CSVRQLQAWNGLKNSSRLKLGQVLHVASPETVRAVNAANAAANAAAHAAAVAAKAAAA
                     SAATGAQAAAQSSAQASAATGARATAQSSAQTTAATGAQAAAQSAAQAAAQSTAQATA
                     QSATQPSAPSAAEAREVARQTSRHANGVSLAWPAGGRVVEAFQPGETRGIEIGGKAGD
                     PVRAAADGKVMYAGTGLNDYGSLIIVQHNKDFLTAYSHNRKLLVKIGDIVRQGQQIAE
                     MGDENNSRVSVGFELRRDGKPIDPMPYLPQGRG"
     gene            complement(2089..2314)
                     /locus_tag="VHV99_21355"
                     /pseudo
     CDS             complement(2089..2314)
                     /locus_tag="VHV99_21355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491613.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
     gene            complement(2825..3223)
                     /locus_tag="VHV99_21360"
     CDS             complement(2825..3223)
                     /locus_tag="VHV99_21360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637980.1"
                     /translation="MNDQQCTEFLSEVRRPLLALHKAILDHERAAYEKEFGPVTPAAF
                     LQVLINGSGFRWLTPLSTVIANVDEILDDKEADDADRLAAVEAVTGLFSPEQPNNAFL
                     PRYLPLLHADPAILHHHGQVSQLLRSAEAK"
     gene            complement(3344..3622)
                     /locus_tag="VHV99_21365"
     CDS             complement(3344..3622)
                     /locus_tag="VHV99_21365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637981.1"
                     /translation="MRLAVPCCTELFPTRRVAPAHIREKAATVPFRLPFFLLPQRFDY
                     FAAQVDFRNEGAGRSAACPRNEKDAAAAFAGSETNFKSMRYHYRIGVI"
     gene            complement(3624..4280)
                     /locus_tag="VHV99_21370"
     CDS             complement(3624..4280)
                     /locus_tag="VHV99_21370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX3637982.1"
                     /translation="MVEFTLWDIARNLLLAARWTVLLSLIAFVGGGVVGLILLAMRVS
                     PIAWLRRIVVVYVEVFQGTPLLMQLFLTFFGLPLLGVDVSPWVAATVTLTLYTSAYLV
                     DIWRGCVEAVPRGQWAAGASLGMTFGQQLRYIVWPQALKIAVAPTVGFLVQVVKSTAL
                     ASIIGFTELTKTGTMITNAAFRPFPIYGMVAMIYFAMCFPLTWYARVLEKRQKVGQHR
                     "
     gene            complement(4283..5029)
                     /locus_tag="VHV99_21375"
     CDS             complement(4283..5029)
                     /locus_tag="VHV99_21375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX3637983.1"
                     /translation="MTYAFDFSGFGLYAGLLARGVAVTLGLTAVSTVLGGLVGIGGAS
                     IAVAGPKWARWVVATYVELIRNTPFIVQLFFIFFGLPSLGIHIDEVQASILAMTVNLG
                     AYAIEIVRAGIDSISRGQVQAAQALGLHGRQVFRYIVLPQALANVFPALLSQVLIVML
                     GSAVVSQISVPDLTYAANFIQSRNFRSFESYLIITATYLALSILLRQLLNRFGRGLFA
                     GRAARGADNAPRNWRTRLMWRGARTLPTER"
     gene            complement(5149..5973)
                     /locus_tag="VHV99_21380"
     CDS             complement(5149..5973)
                     /locus_tag="VHV99_21380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX3637984.1"
                     /translation="MNQPQVARNSTRRVVATLAATLAFTGLGALCAFPSAAKADALDT
                     IAKSGTVRIGVFEDYPPFGSIGPDMKPMGYDIDVANLIGKALNAKVELVQVTGDNRMA
                     YLADHKADMLLSVGQTPEREKVIDFSQPYAPYYLAVFGPKALPVKNAADLAGKSISVA
                     RGTLEDLSVTKIAPPTATIKRFDDPNGAISAFLSGQVQLMVVGNDVGATILARHPAND
                     PEQKFSLFSSPDHVGLNKNEPRLKQKVDETIAKARKDGTMNAISQKWLRAPLPADL"
     gene            complement(6032..7105)
                     /locus_tag="VHV99_21385"
     CDS             complement(6032..7105)
                     /locus_tag="VHV99_21385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3637985.1"
                     /translation="MTHLTLQAVTKRFNAAYAVDSVDLSVPDGKLVCFLGPSGCGKTT
                     LLRMIAGLETPSSGSIDFAGRDITHLPANQRDFGMVFQSLALFPHMTVAQNVAYPLRL
                     RKTAKDAQARRVTELLELIQLPHMANRPVTQLSGGQRQRVAIARAIASQPKLLLLDEP
                     LSALDAKLREAMQVEIRLLQQRLGITTIMVTHDQREAMTMADEIVVMEKGRIAQVGKP
                     LDIYRDPVSEFVADFIGLGNILPVTYDGAGGVSLPGGRRIAVAQPARVPESHSDIRLL
                     IRPEDVCVKSASADAGPNRLAGTVTFIRDVGASLEATIDCAGFTLTAATTPRETPGLT
                     LGMPVLAELPAHACKLIAARAAR"
     gene            complement(7164..8063)
                     /locus_tag="VHV99_21390"
     CDS             complement(7164..8063)
                     /locus_tag="VHV99_21390"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3637986.1"
                     /translation="MSTPIQTPRLTRTPGSPGAPRLPRLPTTMPGQLGKATALLAAIV
                     LFLAALPILTMITMSFSAADTLEFPPHAYGLHWYRAAWHSFVSPDASDSLSMGAALST
                     SLIVALSTMVIATLVSVPAAYALSRYRFRGKPAVEQLVALPLVYPLVMLGLSLLLVFN
                     VLPVELGVFRLIIAHVILALPFTVKNCAASVASIGPEFEEAACVMGASPTRALIDVIL
                     PLMRPGILAGMLFAFIVSFNEFTVTFFLYGIDTMTLPVWLYSRTVSSLDPTVFCFAVF
                     IVAIDFALIWLLEKLIGDEGVAL"
     gene            complement(8060..9001)
                     /locus_tag="VHV99_21395"
     CDS             complement(8060..9001)
                     /locus_tag="VHV99_21395"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3637987.1"
                     /translation="MRGSPSDAVSGTASDVRRASADAPHAPTGAIMTKARPWLLLTPI
                     LLFLAILGAAALVVLRMSFGTQGNEWRGFTLQNYADLLDGYFLKSLWLTLRLAFQSMV
                     CAVLLAIPVALAMARTKSRLARRLLLAGVLLPLLVNLLLQGYGWLIILGPAGLLNHAL
                     LGSGLVDRPVMWLYREHGVLLGLIQTAFPLAVLPLSSAMRAVSSSYEEAAATLGATRW
                     QTLRHVLLPLAMPGLVSGALLVFAYNASAFAVPLLLGGRRVPMLAVLVHDQVAPLLNW
                     PAASASGVVLMIATLTVMALSQRLVRRTQRLAEEPHA"
BASE COUNT         1868 a         2823 c         3025 g         1557 t
ORIGIN      
        1 tgttctgccg gtgctgcact tcacgcatca tccggtcggc ttcgtgctgt atcacggcaa
       61 gacctacagt tagttgccgg tcttatcgag ccacggcgga cagctggtcg acttcggttt
      121 tatcgagcgg gacgggcatg ccagggtccg ctcgccagca cattttcgct ccgcgccctc
      181 cgtcgcggcg atcggccatt caagttttgg cggcgctttg ccgttaatgc aaacgttgtc
      241 gtccggttgc ccggcggcgc agccgtttac gggcatttgc gaccgtgcgc gtagaggcct
      301 caggccgacg gtgcatatga ggcgcggaca gctccgatgc agcggctgat ttagccagag
      361 cggcggcgcc cggatctgca cggagctggg gtggttcgag ggcaaacggc gcgggactgg
      421 ttgcatacac aaccatgtcg caatacggcg agtaagcttt gtatcagatt gtttggagcg
      481 gagcgttttc tgtaaaacgc actttacaat tccgcgtgaa ttttaatcgg caatctgctc
      541 ttttcgcgga agatcgcctg tttgcggtat cgggtgcttc gtgtccggga tctggaatcc
      601 cggctgtccg gtatccgaat agccgggctt tcggcaaggc gaaaggggtt ggtttgaacg
      661 gcagggcatg ggtgggcgcc gcacgaggcg gactcgctgt gtcgcgaacg aaagcagcgc
      721 ttcacggaac gcgcgcatcg taagcgacgg ggcagcaccc ggctcgccaa taagaaagca
      781 ggagtcgaac aatggtggga aagcgcttcg acagaacaat gatggccagt ctggccggcg
      841 tgtgggtcgc gctggcggtc agcggttgtg cgaatgtggg gcagcagggc gcgcagacgg
      901 gcgcggtatc aggagcatcg ggtgcatcgg ctgcgtccag tgtgccggcg gcggctgctg
      961 tgcccgcctc cgatgcgctc gcaggcacac aggtcaagct ggccgctgac gaaggcacgc
     1021 cgcttaaaaa agcgccgccg ttaacttatc gcgtcaagcg tggcgacacg ttggcgcgca
     1081 tcgcgcagca tcatcattgc agcgtcagac agttgcaggc atggaatggc ctcaagaatt
     1141 cctcgcggtt gaagcttggg caggtgctgc atgtcgcctc gccggaaacg gtgcgggcgg
     1201 ttaatgcggc caatgcggcg gccaatgcgg ccgcccatgc ggcggcggtc gctgcaaagg
     1261 ctgcggcggc gtcggcggca accggcgcgc aggctgcggc gcaatccagc gcgcaggcgt
     1321 cggcggcaac cggcgcacgg gcaacggcgc aatccagcgc gcagacaacg gcggcaaccg
     1381 gcgcgcaggc agcggcgcaa tccgccgcgc aggcagcggc gcaatctacc gcgcaggcga
     1441 ctgcgcaatc agccacgcaa ccatccgcgc ccagcgccgc cgaggcgcgc gaagtcgcgc
     1501 ggcagacctc ccggcacgcg aacggggtgt cgctcgcatg gcctgccggc ggccgcgtcg
     1561 tcgaggcttt ccaaccgggc gaaacacgcg gcatcgaaat cggcggcaag gcgggcgatc
     1621 cggtgcgggc cgcggccgac ggcaaggtga tgtacgcagg cacgggcctg aacgactacg
     1681 gcagtctgat catcgtccag cacaacaagg actttctgac cgcctactcg cataaccgca
     1741 agctgctggt caaaatcggc gacattgtgc ggcaaggtca gcagatcgcc gagatgggcg
     1801 acgagaacaa ctcgcgcgtc tccgtcggct tcgagttgcg ccgtgacggc aagccgatcg
     1861 atccgatgcc gtatttgcca caaggccgcg gctaggtctg cgcccaggct cccggcaggc
     1921 tcgcggcccg cgtctcggac aaaaataaga gccggcgatt tttcgtctgc tctacacaaa
     1981 cagcgaaccc ggcaaattat ttgtcgggtt cgctgctttg tcacatccgt ccgtccggaa
     2041 gcggtagtga gcaaaacgcc gggtttgctg cttcgtcgca tccacttgtc cgagcgagac
     2101 gttctgagcg tcgcgctgcc cgaattggcc gtagcccagc agatcgtaga aatagacgga
     2161 gcgaatctgc gagatatggg gcgatgcgat gccacacata tgaagagaac ggcgtgccat
     2221 gaaccagcac cagcagcgga ccattgccgg ttacgccaaa gcgcacggtt tgatcgcgga
     2281 atggatagct ttcgggaagc cgccagcttt gcatagcggt gagcgtcgcg agaaagtctg
     2341 cttaccttgg caggcgtgtc caaagcacgc tgagcgcttt ttcgtagcgt tcctggcgag
     2401 gctcccgcaa aagctcacct ttgacacatc tttgttttag tcagcaaagc caggtaaatt
     2461 gaaaagaatg tcactcacgg cgtcgccgga ttgatcgctg agcgacacag gcacagcgca
     2521 ctgttacgaa tcaagcacaa atcaccttga accaaggtgt ctttgcgttg ggcgcgttcg
     2581 gaaagcctgg taatcgtcga agacgcatcg attccttatt caacaagaag acgcgcgatc
     2641 gaccgaaatg cacgcatggc aatggtgagc gaattcggga gccgcacgca caaccatcgg
     2701 cgggcgccgc acggtatcgc gccaggcgcg gcccccaatt gcaccacgcc cggcggtcgc
     2761 gtttaaccgc agcgacgacg tccacctcac gccaacgaag cgggcgagtc aacgcacccg
     2821 ctgcctattt cgcttcagcg ctgcgcagca gttgcgagac ctggccgtgg tgatgcagga
     2881 tcgccgggtc ggcgtgcagg agcggcaagt agcgcggcag gaacgcgtta ttgggctgct
     2941 cgggagaaaa gaggccggtg accgcttcaa ctgcggcgag ccgatcggcg tcatccgctt
     3001 ccttgtcgtc gagaatctcg tcgacattgg cgatgaccgt tgagagcggc gtgagccagc
     3061 gaaaccccga gccgttgatg agcacttgca ggaaggccgc cggcgtcacg gggccgaatt
     3121 ccttttcgta cgccgcgcgc tcgtgatcga gaatcgcttt atgcagcgcc agtagtggtc
     3181 tgcgaacttc cgagaggaat tcagtgcatt gctggtcgtt catcttgccg ctccttcgga
     3241 tagccatgcc ggatcgagcc gggcgatcgg gtcaagccag atccatgcta acaaaatcgt
     3301 tcgaggatgc atcagtaaga atgatgcgcg tacgcgaagc acgtcagatc acgccaatgc
     3361 ggtaatggta acgcattgat ttgaaattcg tttcacttcc agcaaaggct gcggcggcgt
     3421 ctttttcgtt gcgtggacat gccgcggacc tgcccgcccc ttcgttgcgg aagtcgacct
     3481 gggcggcgaa gtaatcaaac cgttgcggca ataaaaaaaa cggcaaccgg aacggaacgg
     3541 ttgccgcttt ttcgcggatg tgtgcggggg ctacccggcg tgtcgggaac agttcagtgc
     3601 agcaaggaac agctaaccgc aagtcaccga tgctgcccca ccttctgccg cttctccagc
     3661 acgcgcgcat accacgtcaa cggaaaacac atcgcgaagt aaatcatcgc gaccatgccg
     3721 tagatcggga acgggcggaa cgctgcattg gtgatcatcg tgccggtctt ggtcaactcg
     3781 gtgaagccga tgatcgacgc cagcgccgtg cttttcacga cctgcacgag aaagccgacc
     3841 gtcggcgcca cggcgatctt cagcgcctgc ggccagacga tatagcgcag ttgctggccg
     3901 aacgtcatgc cgagacttgc tcccgcggcc cattgaccgc gcggcacggc ttccacgcag
     3961 ccgcgccaga tatcgaccag ataggcgctc gtataaagcg tcagcgtgac ggtcgccgcc
     4021 acccagggcg aaacatcgac acccagcaag ggcaggccga agaacgtcag gaacagttgc
     4081 atcagcagcg gtgtgccctg aaacacttcc acatacacga cgacaatccg gcgcagccac
     4141 gcgatcggcg acacgcgcat cgcgagcagg atcagcccga ccacaccgcc gccgacaaac
     4201 gcgatcaacg acaacagaac cgtccagcga gcggcgagca gcagattgcg ggcgatgtcc
     4261 cacagtgtga attcgaccat gatcaacgct ccgtcggcag agtgcgggcg ccgcgccaca
     4321 tcaggcgggt gcgccagtta cgcggtgcgt tgtcggcgcc tcgcgcggcg cgcccggcaa
     4381 acagtccgcg gccaaaccgg ttcagcaatt ggcgcaacag aatcgacaag gccagatacg
     4441 ttgcggtgat gatcagatag ctctcgaacg agcggaagtt gcgcgactga atgaagttgg
     4501 ccgcataggt cagatcgggc acggagatct gcgaaaccac cgccgaaccg agcatgacga
     4561 tcagcacctg gctcaacagc gcgggaaaca cgttggcgag cgcctgcggc agcacgatgt
     4621 agcggaacac ttgccgtcca tgcaggccga gcgcttgcgc tgcctgcacc tggccacgcg
     4681 aaatcgaatc gatgccggcg cgcacgattt cgatcgcata cgcgccgaga ttgacggtca
     4741 tcgccagaat cgaggcctgc acctcgtcga tatgaatgcc gaggctcggc aagccgaaga
     4801 agatgaaaaa caactgtacg atgaacggcg tattgcggat caactcgacg taagtcgcga
     4861 ccacccagcg cgcccacttc ggcccggcca ccgcgatact cgcgccgcca atcccaacga
     4921 gaccgccgag cacggtggat acggcggtca gcccaagcgt gacggcgacg ccgcgcgcga
     4981 gcagccccgc atagaggccg aagccgctga aatcgaacgc ataggtcatg gcgtggcgct
     5041 catcataagg tcaaactccg gcgcaaccgc cccgaaggaa gtccccttgg gggtgccccg
     5101 aaggacgccc ccaaggaacc ggcttggtcg gttcgcgcaa taaaaggttc agaggtcggc
     5161 cggcagcggc gcacgcagcc atttctgcga aatcgcattc atggtgccgt ccttgcgggc
     5221 cttggcaatc gtttcgtcga ctttctgctt cagacgcggc tcgttcttgt tcaaaccgac
     5281 gtggtccggc gagctgaaca gcgagaactt ctgctccgga tcgttcgccg gatgacgcgc
     5341 gagaatggtt gcgccgacgt cgttgccgac taccatcaac tgtacctgac cagaaagaaa
     5401 tgccgaaata gcgccgttcg gatcgtcgaa acgtttgatg gtcgccgtgg gcggggcgat
     5461 cttggtcacg ctcaggtctt ccagcgtgcc gcgcgcgacc gaaatcgact tgccggcgag
     5521 gtcggcggcg ttctttacgg gcagcgcttt cgggccgaag acggcgagat agtagggcgc
     5581 ataaggctgc gagaagtcga tcactttttc gcgctccggc gtctggccga ctgaaagcaa
     5641 catgtcggcc ttgtgatcgg cgaggtaggc catgcggtta tcgcccgtca cttgcacgag
     5701 ttcgactttt gcgttcaacg ccttgccgat cagattggcg acgtcgatgt cgtagcccat
     5761 cggcttcata tccgggccga tcgagccgaa cggcggatag tcctcgaata cgccgatgcg
     5821 caccgtgccg gacttcgcga tcgtgtcgag cgcatccgcc ttcgcggccg acggaaacgc
     5881 gcacaatgcc ccgaggccgg tgaaggcgag cgtggcagcg agggtggcga cgacgcggcg
     5941 ggtgctgttg cgggcaactt gtggctggtt catggtcttt cctctgtcga aaactcggta
     6001 ttggaatggg tatctgtgag cgctgcgcgg gttaacgcgc cgcccgcgca gcaatcagtt
     6061 tgcacgcgtg cgcgggcaat tccgcgagca ccggcatgcc gagcgtcagt cccggcgttt
     6121 cgcgcggggt ggtcgcggcg gttagcgtga agccggcgca atcgatcgtc gcttcgagcg
     6181 acgcccccac gtcgcggata aaagtcaccg tgcccgcgag ccggttcggc ccggcatccg
     6241 cggaggccga tttgacgcac acgtcctccg gccggatcag cagacgaata tcgctatggg
     6301 actccggcac ccgcgccggt tgcgcaacgg cgatgcgccg gccgcccggc agactgacac
     6361 cgcccgcgcc gtcgtaggtg accggcagaa tgttcccaag accgatgaaa tcagcgacga
     6421 attcgctgac cgggtcgcga tagatatcga gcggtttgcc gacctgcgcg atccggcctt
     6481 tctccatcac cacgatttcg tcggccatcg tcattgcttc gcgttgatcg tgcgtgacca
     6541 tgatcgtcgt gatgccgagg cgctgttgca gcagccggat ttcgacctgc atcgcttcgc
     6601 gcagtttggc gtcgagcgcg gaaagcggtt cgtcgagcag cagcagcttc ggttgcgacg
     6661 cgatcgcgcg cgcaattgcc acgcgttgcc gctggccgcc ggagagttgc gtgaccggcc
     6721 ggttcgccat atgcggcaac tggatcaact ccagcaattc ggtgacgcgg cgcgcttgcg
     6781 cgtctttcgc ggtcttgcgc aacctgagcg gatacgcgac gttttgcgcg accgtcatat
     6841 gcggaaacag cgcgagcgac tgaaacacca tgccgaagtc gcgctggttg gcgggcagat
     6901 gcgtgatgtc gcggcccgcg aaatcgatgc tgcctgagct cggcgtttcc agtccggcga
     6961 tcatccgcag cagcgtggtc ttgccgcagc cggacggccc gagaaagcac accagcttgc
     7021 cgtcgggcac gctcagatcg acgctgtcga cggcatacgc cgcgttgaag cgcttggtca
     7081 cggcctgcaa ggtcagatgg gtcatcgaat gctccagcaa gaaggcgtca tcgcagcttg
     7141 aattgaaggg tggtccaagg cgttcaaagg gccacgcctt catcgccgat cagcttttca
     7201 agcagccaga tcagcgcgaa gtcgatcgcg acgatgaata cggcgaaaca gaacacggtc
     7261 ggatcgagcg atgacaccgt gcggctgtag agccacaccg gcaacgtcat cgtgtcgatg
     7321 ccatacagga agaacgtcac cgtgaattcg ttgaacgaga cgatgaacgc gaacagcatg
     7381 ccggcgagaa tgccggggcg catcaacggc aggatcacgt cgatcagtgc gcgggtcggg
     7441 ctcgcgccca tcacgcaggc ggcttcctcg aactcggggc cgatcgaagc caccgacgcg
     7501 gcgcaatttt tcacggtgaa cggcagcgcc agaatcacat gcgcgatgat caggcggaat
     7561 acgccgagtt cgaccggcag cacgttgaac accagcagca gcgacaagcc gagcatcacc
     7621 agtggataga cgagcggcaa cgccaccagt tgttcgaccg cgggcttgcc gcggaaacgg
     7681 tagcggctca gtgcgtaggc ggccggcacc gagacgagcg tggcgatcac catggtcgag
     7741 agcgcgacga tcaggctcgt gctgagcgcg gcgcccatcg agagcgagtc gctcgcgtcc
     7801 ggcgagacga agctgtgcca cgccgcgcga taccaatgca ggccgtaggc gtgcggcggg
     7861 aattcaagcg tgtcggcagc gctgaacgac atggtgatca tcgtcaggat cggcagcgcg
     7921 gcgaggaaca gtacgatcgc ggcgagcagc gcggtggctt tgccgagctg acccggcatg
     7981 gtcgtcggca agcgcggcag gcgcggtgca cccggtgagc ccggtgtacg tgtcaggcgc
     8041 ggagtctgga tcggcgtgct catgcgtgag gctcctcggc gagacgttgg gtacggcgca
     8101 cgagccgctg cgacagcgcc atcacagtca gcgtcgcgat catcaatacg acgcccgaag
     8161 cggaagcagc gggccagttc agcaagggcg cgacctggtc gtggaccagc accgcgagca
     8221 tcggcacacg gcgtccgcca agtagcaggg gcacggcgaa ggcactggcg ttgtacgcga
     8281 acacgagcag ggcgccggac acgagccccg gcatggcgag cggcagcaag acgtgacgca
     8341 gcgtttgcca acgcgtcgcg cccagcgtgg ccgcggcttc ttcatacgag ctcgagaccg
     8401 cgcgcatggc actcgacaac ggcagcacgg cgagcggaaa cgccgtctga atcaaaccga
     8461 gcaacacgcc gtgttcgcga tacagccaca tcaccgggcg atcgacgagg ccgctgccga
     8521 gcagtgcgtg attgagcagg ccggccggac cgagaatgat cagccagcca taaccctgca
     8581 acagcagatt gacgagcaag ggcagcagca cgccggccag caacaggcgg cgcgcgagcc
     8641 ttgacttggt gcgcgccatc gccaaagcga cgggaatcgc cagcagcacg gcgcagacca
     8701 tgctctggaa cgcgagcctc aacgtgagcc acaacgactt caggaaatag ccgtcgagga
     8761 gatcggcgta gttctgcagc gtgaagccac gccattcgtt gccttgcgtg ccgaagctca
     8821 tgcgcagtac gacgagcgct gccgcgccga gtatggcgag aaatagcagg atcggcgtga
     8881 gcagaagcca gggacgcgct ttcgtcatga tggcgccggt gggggcgtgt ggtgcgtcgg
     8941 cagaggcgcg gcgtacgtcg gacgccgttc ccgatacggc gtcggaaggg gaaccgcgca
     9001 tgccggacgg ggcgtcggac gccgttccca atgccacgtc gggcggtgcg tcggatggcg
     9061 ttttagaagc cgtgtcctga gccgtttccg aggccacgtt cgacgccact tccgagcccg
     9121 cttccgagcc cgatgaggca cgccgcgaaa agcgcgcgag catcgcggtc acgccgcgaa
     9181 aatttcggtg tagcgcttga tccatgccgg ctgcacgacg gcgagcgcct tgtcatcatg
     9241 caggatcgct ttctcggcaa tctgctgcgg gct
//
LOCUS       DATASH010000797        15764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3099386,
            whole genome shotgun sequence.
ACCESSION   DATASH010000797 DATASH010000000
VERSION     DATASH010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 15764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15764
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3099386"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VHV99_21400"
     CDS             complement(<1..195)
                     /locus_tag="VHV99_21400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3637988.1"
                     /translation="MSSTQLRVFLLFSAGYFVSYVFRGVNLGFAPFITHDLGLSATDL
                     GVLTSLYFLGFAGAQIPAGVL"
     gene            complement(398..2092)
                     /locus_tag="VHV99_21405"
     CDS             complement(398..2092)
                     /locus_tag="VHV99_21405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX3637989.1"
                     /translation="MRFIFSALLVLTLAACASQGPATNSPTTATNPASQQAVADALRK
                     TATAKETINVDQGSVTQLTSAYADLWGRIRRGFQMPDLQTDLVDMQVNWYAQRPDYVQ
                     RMTERSQKYLYHIVEELEARHMPTELALLPFIESAYSPQALSVAKAAGMWQFMPGTGR
                     TYNLKQNMWQDERRDVLASTSAALDYLSNLHDMFGDWQLALAAYNWGEGNVQRAIARN
                     EAAGLPTDYLSLRMPNETRNYVPKLQAVKNIVMNPQMYGLTLPSIPNHPYFVTVTTSH
                     DIDVDMAAKLANLSTDEFRSLNPSFRKPVILGATQPQILLPFDNASAFERNLKAYSGS
                     LSSWTTYTVTERAAPAAIAQKIGVDADTLMEVNKIPAGMRLKPGSTIVVPRGSDDDED
                     ISADVAESAVLAMEPDVPDTRKMLIRVRRNQTMAAVAVRYGVSIGQLKAWNRTHRDQV
                     SRGQVIVLHVPVGKAMPSEPGPERIATDVQGGGVEKIGTRVADTRNDSRYDKNKGRGR
                     SAVVKVSEPVGKASKPTTSVASKDKVTKVSASTSSKASKAAADSRPKTAANAKKGK"
     gene            complement(2249..3040)
                     /gene="gloB"
                     /locus_tag="VHV99_21410"
     CDS             complement(2249..3040)
                     /gene="gloB"
                     /locus_tag="VHV99_21410"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015600438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEX3637990.1"
                     /translation="MNALEYVPVPAFEDNYIWVVSDGHHAVVVDPGDAAPVRAYLAKR
                     GWRLSAILLTHHHQDHVGGVADLLNGQAVPVYGPAGEAIQHLTHRLENGDHVTIAAPA
                     LEFSVLDVPGHTSGHIAYFQAADPRGTPHVFCGDTLFACGCGRLFEGTPTQMLASLDS
                     LAALPGATEVHCAHEYTLSNIRFALACEPGNAELQAWRDKASGLRARHVPTLPTTIAH
                     ERAVNPFLRADSPVIQTTLQEQLHEKVPDRLTAFTLMREWKNRFR"
     gene            3060..3935
                     /locus_tag="VHV99_21415"
     CDS             3060..3935
                     /locus_tag="VHV99_21415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3637991.1"
                     /translation="MTDRAIIDWPAWTDSPPGRYVLDWEQTQLDRVVSDVFGYHALQL
                     GLPQLDALRENRMPCRGLVLDAASGASAPYTFPRGLGRAGNGLANGSAIDSRKDAAQG
                     LPAGLSAPAGRSAVWCDLLDLPFEAQSVDLIVMPHTLEFTSDPHRLLREAERVLMPEG
                     QLIILGFNSLSLWGARQSVGKMTGRPFVPAAVDLIAFTRLKDWIKLLGFDLERGRFGC
                     YRPPLGSDQWLSRYGFMEAAGDRWWPIFGATYMIKAIKRVRGMRLVGPLKVKKPVLAA
                     GLAPAATPNTRNHTQ"
     gene            3932..4378
                     /gene="rnhA"
                     /locus_tag="VHV99_21420"
     CDS             3932..4378
                     /gene="rnhA"
                     /locus_tag="VHV99_21420"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEX3637992.1"
                     /translation="MTSDIIDIYTDGACKGNPGRGGWGALLRFGDQEKELFGGEANTT
                     NNRMELMGVIAALEALKRPCKAVVHTDSQYVQKGISEWIHGWKKKGWITAAKQPVKNA
                     DLWKRLDALVAQHEIEWRWVRGHNGHPENERADQLANRGVASLAEI"
     gene            4460..5191
                     /gene="dnaQ"
                     /locus_tag="VHV99_21425"
     CDS             4460..5191
                     /gene="dnaQ"
                     /locus_tag="VHV99_21425"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEX3637993.1"
                     /translation="MRQLILDTETTGLNARTGDRILELGCVEMVNRRLTGNNLHFYIN
                     PERDSDPGALAVHGLTTEFLSDKPKFAEIADQFRDFIQDAELIIHNAPFDIGFLDAEF
                     ALLGLPPVKTYCGEIIDTLARAKQMFPGKRNSLDALCDRFGISNAHRTLHGALLDSEL
                     LAEVYLAMTRGQESLVIDMLGDSHGGGAHAPRIAIDSLNLIVIAASDDEVAEHQSVLD
                     GLDKAIKGTSVWRLEPAPAADEQTA"
     gene            5240..5314
                     /locus_tag="VHV99_21430"
     tRNA            5240..5314
                     /locus_tag="VHV99_21430"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5272..5274,aa:Val,seq:cac)
     gene            complement(5631..11210)
                     /locus_tag="VHV99_21435"
     CDS             complement(5631..11210)
                     /locus_tag="VHV99_21435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3637994.1"
                     /translation="MSSSFRLDMSGHSHFQVIREDGERTWCRVRRNDADGNEHVVLAV
                     RLTAERSSAADLDRLAHEYGLKEELDNAWALRPLELLREGRRTLLLFEDFEGEPLDAQ
                     LGAPLEMGRFLRVAVGIVTAVGQVHQRDLIHKDLKPSHILVNGSTEEVRLTGFGIASR
                     LPREWQTPEPPELIAGSLAYMAPEQTGRMNRSIDARSDLYALGVTLYQMLTGSLPFTA
                     TDPTEWVHCHVAKTPDAPNERVKNVPDVVSAIVMKLLAKTAEERYQTAGGVESDLRRC
                     LAEWQARGSIHTFAPGRDDTSDRLLIPETLYGREREIDTLLAAFERVVAGGGPELVLV
                     SGYSGIGKSSLVQELHRAVVSPHGLFASGKFDLQQRDIPYATLAQALQSLIRPLLGQT
                     DAELAPWREALAEALGPNGQMMMPLIPELRLFVGPQPPAPELPPQEAQRRFQLVFRRL
                     LGVFARAEHPLALFLDDLQWLDLATLDLLEHLVTQRELRHLLLVGAYRDNEVTPAHPL
                     MRRLAAIRSAGGQVQEILLTPLSVAGLGRLIADALQCAPQRAAPLARLVHVKTGGNPF
                     FAIQFLTVLWEEGLLAFVPGRRTWAWDLERIRAKGYTDNVVTLLLGKLARLPEETRDA
                     LTRLACLGTSAQTATLGTVLGVSEKAVHAALWDAVRSGLVLRSDRAYAFLHDRVQEAA
                     YALIPAGERPTQHLQIGRRLGLWTAPEKVEDNIFEVVNQLNRGAGLLISVEERQRVAE
                     LNLMAGKRAKQSMAYHSALTYLAAGRALLAEDCWDRRYALAFSLEFHHAECEFLTGQL
                     TGAEARLAALPTHAASLVDLAAVTRLRISLYMTAGENGHAIAVGIEYLRRVGIDCSPH
                     PTHDEVRKEIEHIWRQLGKRSIEALIELPPMSDPAACASMDVLAEILPPASWTDNDLY
                     CLVVCRMANLSLQYGNSDASCLAYVYLGMILGPHFGDYRAGFRFGQLAIDLVEKRRLD
                     RFKARVYMCFGYLVNPWLRHLNTCRSLLRRAFDMAQEAGDLTFTGYSYINTVTFMLAG
                     GDPLDEVQREAEAGLGFVRKAQFGVVVDLVTAQLGLIRTLRGQTPVFGCFNDDQFDED
                     RFEQHLQGDPGLAVVTCRYWIRKLQARYFALEHACAVAAAAKAEQWLWTSLSFFEVAE
                     YHFYSALAHAAQCDMTSVEERAEHLDALAAHHRQLQEWAEHCPANFSDRAALVRAELA
                     RLEGRELDAERFYEQAIRAAQDNGFVQNASLAFELVARFYAARGLDSFAGTYLRRARQ
                     GYLRWGAEGKVRQLDQLHPQLREDEPAPGPTSTIAAPVEHLDLATVIRVSQAVSGEIV
                     LDKLIDTLLRTALAQAGAGRGLLFFVAHGGEPRLRAEATIERDTVVTQLRDEPVTTAM
                     LPESILRYVMRTRENVILDDAIKHDVFCADPYIQQHQVRSALCLSLINQGGLVGMLYL
                     ENNLTPRVFMPTHVAVLKLLASQAAITLENALLYRDLAEREARIRRLVDANIVGIFIW
                     DLAGQILDANDAFLRIVGYGREDLVSGRMRWTDLTPPEWLDRDIQQRIPELRMTGTLQ
                     PFEKEFARKDSSRVPVLVGAATFEEGASQGVGFVLDLTERKRAEAEARESERRYREVQ
                     TELEHSNRAATMGQLMASIAHEVQQPIAATATSASAALRWLAANPANLAEVRQALDLI
                     VNEAIRAGGIVSGIRDLVRKAPPRKDRVDINEAVREVIELTRGEAAKNDVSVLTVLGD
                     GLPLVLGDRVQLQQVMLNLIVNAIEAMSETSMGPRELLISTTADSSNGVSIAVRDSGP
                     GLPEDGIERAFDPFYTTKADGLGMGLSICRSIVEAHRGRLWASANAPRGAVFQLVLPR
                     GEVEHAPSSENDGLPVA"
     gene            complement(11620..12426)
                     /locus_tag="VHV99_21440"
     CDS             complement(11620..12426)
                     /locus_tag="VHV99_21440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3637995.1"
                     /translation="MTRVLITGAAGHIGRVLRAGFQGHYALRLADIAPQAEAQAREEI
                     VTADITRLEDLLPVMEGVDVVVHLAGVPDEAPWERIRDMNVDGCYNVFEAARRAGVKR
                     VVFASSNHAVGFHRRDRMIDNTVEPRPDSRYGISKVFGEALGRMYADKHGMSVACIRI
                     GSFRPDDQPTAPRHLFTWISHRDMVQVTRRCIDYPDFHFAVVYGVSANARNRWDNANV
                     RFLGFEPEDDAELHAAQIAATGKAEDAREALFHGGFGCLVEFDGDPQRID"
     gene            complement(12458..12646)
                     /locus_tag="VHV99_21445"
     CDS             complement(12458..12646)
                     /locus_tag="VHV99_21445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637996.1"
                     /translation="MFLQYLEQARAERHQMIGSRRTGDREPSVGLRREHWVSWRDACL
                     EAQHRRSFGYAAQKSEAD"
     gene            complement(12716..12901)
                     /locus_tag="VHV99_21450"
     CDS             complement(12716..12901)
                     /locus_tag="VHV99_21450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637997.1"
                     /translation="MSNISGIVGLTMMGIAVQYFGGYNSGFVHGAAVDLLAVLAMLLV
                     IGRRAAGEAGETPTTAA"
     gene            13045..13257
                     /locus_tag="VHV99_21455"
     CDS             13045..13257
                     /locus_tag="VHV99_21455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637998.1"
                     /translation="MLTPSRATPESASGTIAATLRASNLLLMRLKNASATSPPPKYPI
                     PPHAHGNGTALRMLMCSMSRCSSTGQ"
     gene            13364..13636
                     /locus_tag="VHV99_21460"
     CDS             13364..13636
                     /locus_tag="VHV99_21460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3637999.1"
                     /translation="MYLRTGSPFLIGIAAILQPAVTWLRQTKSSNGTGVPIAASMRAI
                     TTLSAGCNQITGPVTLARHLSHQALPFEKFALDGQKGSDTSNRFLK"
     gene            complement(14028..15077)
                     /locus_tag="VHV99_21465"
     CDS             complement(14028..15077)
                     /locus_tag="VHV99_21465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013699837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX3638000.1"
                     /translation="MEQNEVILGVDTHLDTHVGAVISDTGRLLGTLSVSTDTAGYLDL
                     LTWANSFGPLRRAGVEGTGTYGAGLARLLRDHEIEVLEVNRPDRAMRRSRGKSDPTDA
                     ENAARAVLSGRATAIPKEQSGAAEAMRAVCVARRSAVKAKTQAINQLRALLVSAPQEI
                     RERLLKAKTAECVEGCARLRSLGKTPMLQTLTTTLRLLAKRWLTLAEELKALDSMLES
                     LTSQHARRLRERFGVGPQTAAVLVAVAGDNPERLKNEAALAALCGTSPLQSSSGKTVR
                     HRLNRGGDRSANNALWTIAMVRMRSDPRTRAYVERRTKEGMSNKEIHRCLKRYIVREL
                     YSLILADLADSTLAT"
     gene            complement(15291..15764)
                     /locus_tag="VHV99_21470"
                     /pseudo
     CDS             complement(15291..15764)
                     /locus_tag="VHV99_21470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         3046 a         4974 c         4827 g         2917 t
ORIGIN      
        1 caacacgccg gccgggatct gcgcgcccgc gaagccgaga aaatacagac tggtcagcac
       61 gccgagatcc gtggccgaca agccgagatc gtgcgtaatg aaaggtgcga aaccgagatt
      121 gacgccgcga aatacgtaag aaacaaaata accggcggaa aacaggagaa aaacgcgcaa
      181 ctgggtcgac gacataaact ttgagatgag cggccggatt tggcccaagc acgaaagata
      241 aatgaaacgg gcgaacttcg tccgaattga tctgcagaat gaaaaacgcc gtcaccatgg
      301 gtgacggcgt tggaataaca cggtgcgtga tcggagcgca attcatgcgc tgcccccgca
      361 acgcatgtgg tcactaccgt gggagccgct ttttggtcta cttacccttc ttcgcgttgg
      421 ccgcggtctt cgggcggcta tctgccgcgg ccttcgacgc cttgctggac gtggaagccg
      481 acaccttcgt caccttgtcc ttgctggcaa cggacgttgt cggcttgctg gctttgccca
      541 ccggctcaga cactttcacc acggcggaac ggccacgacc cttgttctta tcgtagcgcg
      601 aatcgttcct cgtgtctgcg acgcgggtgc cgatcttctc gacgccgccg ccttgcacgt
      661 cggtcgcgat gcgctccgga ccgggttcgc tcggcattgc cttgccgacc ggcacgtgca
      721 gcacaatcac ctgaccacgc gaaacctgat cgcgatgcgt gcgattccac gccttcaact
      781 gaccgatgga gacgccgtaa cgcacggcga cggccgccat cgtctgattg cgacgcacgc
      841 ggatcagcat cttgcgggtg tcggggacgt ccggctccat cgccagcacg gcgctttcgg
      901 cgacgtcggc gctgatgtct tcgtcgtcgt ccgaaccgcg cggcaccacg attgtggagc
      961 ccggcttcag acgcatgcct gccgggatct tgttcacttc cattaacgtg tcggcgtcga
     1021 caccgatctt ttgcgcaatc gccgctggcg cggcacgctc ggtgaccgta tacgtggtcc
     1081 acgacgacag ggaaccggag tacgccttca gattgcgttc gaacgcgctg gcgttgtcga
     1141 acggcagcag gatttgcggc tgcgtggcgc cgagaatcac cggctttctg aacgatgggt
     1201 tcagcgagcg gaattcgtcc gtggagagat tcgcgagctt ggccgccatg tccacgtcga
     1261 tgtcgtgcga ggtcgtgact gtcacgaaat acgggtggtt cggaatcgac ggcagcgtga
     1321 gcccatacat ctgcggattc atcacgatgt tcttgaccgc ctgcagcttg ggcacgtagt
     1381 tgcgcgtctc gttcggcatg cgcaggctga ggtagtcggt aggcagcccc gccgcctcgt
     1441 tgcgggcgat cgcgcgctgc acgttgccct cgccccagtt gtaggcggcg agcgccagtt
     1501 gccagtcgcc gaacatgtca tgcagattcg acagatagtc gagcgcggcg ctggtcgacg
     1561 ccagcacgtc gcggcgctcg tcctgccaca tgttctgctt gaggttatag gtgcggcccg
     1621 tgcccggcat gaactgccac atgcccgccg ccttcgcgac tgacaaggcc tgcgggctat
     1681 acgcggattc gatgaacggc agcagcgcaa gctcggtggg catatggcgc gcctcgagct
     1741 cttcgacgat gtgatacagg tacttttgcg agcgctcggt catgcgctgc acgtaatccg
     1801 ggcgctgcgc ataccagttg acttgcatat cgacgaggtc ggtttgcaga tccggcattt
     1861 gaaaaccacg gcgaatacgg ccccacaggt cggcgtacgc gctcgtcagt tgagtgaccg
     1921 agccctggtc gacgttgatc gtttctttgg cggtggctgt cttgcgaagg gcgtcggcta
     1981 cggcttgctg actggcgggg ttggttgcgg tggtggggct attcgtcgcc ggtccctgac
     2041 tcgcgcaagc ggcgagtgtc aggaccaaca acgcactaaa gataaatcgc atgaacgtct
     2101 cggcttccac aagggctgga atttgcagcc gatagtacga aacggcatcg tttacgtcaa
     2161 tcgaaaatta cgaaaaaaat caaggaaatc ttgggcttgg cgaatttttg cgattcatcg
     2221 cgtgagaaat accctgacgc cgaacgggtc aacggaagcg gtttttccat tcacgcatca
     2281 gcgtaaatgc cgtcaaacga tcgggcactt tttcgtgcaa ctgctcctgc aaggtggttt
     2341 gaatcaccgg actgtcggcg cgcaaaaatg ggttgacggc gcgttcgtgg gcgatcgtgg
     2401 tgggcagcgt cggcacatga cgcgcgcgca agccgctcgc cttgtcacgc caggcttgca
     2461 gttcggcgtt gccaggctca caggcgagcg cgaagcggat gttcgacaac gtgtattcgt
     2521 gtgcgcagtg aacctcggtt gcaccgggga gcgcggcaag cgaatccagc gacgccagca
     2581 tctgcgtggg cgtgccttcg aacaaccggc cgcagccgca cgcgaacagc gtgtcgccac
     2641 aaaacacgtg cggcgtgccg cgcgggtcgg ccgcctgaaa ataagcgata tgaccgctcg
     2701 tgtgacccgg cacgtcgagc acgctgaatt cgagtgcggg agccgcaatc gtcacgtgat
     2761 cgccattttc aagacggtgc gtgaggtgct ggatcgcttc accggcgggg ccgtacacgg
     2821 gaacggcctg gccgttcagc agatcggcca ccccaccgac atggtcttgg tgatggtgcg
     2881 tgagtaaaat agcgctcaac cgccaacctc gtttcgccag atatgcgcgc acgggggcgg
     2941 cgtcacccgg atcgacgacc accgcgtgat gcccgtcgga aacgacccag atgtaattgt
     3001 cttcaaacgc cgggactggt acgtactcga gcgcattcat aggcgaccca ttctctgata
     3061 tgactgatcg agcgattata gactggcccg cctggacgga ttcgccacct ggccgctacg
     3121 tgctcgactg ggaacagacc cagctcgatc gcgtggtgtc ggacgtgttc ggttaccatg
     3181 cgctgcaact gggcctgccg caactcgacg ccttgcgcga gaaccgcatg ccgtgccgcg
     3241 gacttgtgct ggacgcggca agcggcgcga gcgcgcccta cacttttccg cgcggtctgg
     3301 ggcgcgccgg gaatggcttg gcaaatggct cggcgattga ttccaggaag gacgccgcgc
     3361 aaggtctgcc cgcgggcttg tcggcgccgg cggggcgtag cgctgtctgg tgcgacctgc
     3421 tcgacttacc gttcgaggca caaagcgtcg acctgatcgt gatgccgcat acgctggaat
     3481 tcacgagcga cccgcatcgg ctgctgcgcg aagccgagcg cgtgctgatg cccgaaggcc
     3541 aactgatcat tctcggcttc aattcgctgt cgctgtgggg cgcgcgtcaa tcggtcggca
     3601 agatgaccgg ccggccattt gtgcccgcgg cagtcgatct gatcgccttc acgcgtttga
     3661 aagactggat caaactgctg ggtttcgacc ttgaacgcgg gcgcttcggc tgctatcgtc
     3721 cgccgctcgg cagcgatcaa tggctgtccc gctatggttt catggaagcc gccggcgacc
     3781 gctggtggcc aatttttggt gccacgtaca tgatcaaggc gatcaagcgc gtgcgcggca
     3841 tgcgcctcgt cggcccgctg aaagtgaaga aacctgtcct cgccgcgggc ctcgcgcccg
     3901 ccgccacccc gaacacacgc aaccatactc aatgacttcc gacatcatcg atatttatac
     3961 cgacggcgcc tgcaagggca accccggccg cggcggctgg ggcgcgctgc tgcgcttcgg
     4021 cgaccaggaa aaagaactgt tcggcggtga agccaacacc accaacaacc gcatggaact
     4081 gatgggcgtg atcgccgcgc tcgaggcact caagcgcccc tgcaaggccg tcgtgcacac
     4141 ggactcgcag tacgtgcaga aaggtatcag cgagtggatt catggctgga aaaagaaagg
     4201 ctggatcacc gcggcgaaac agccggtgaa aaacgccgat ctctggaagc ggctcgacgc
     4261 actcgtcgca cagcacgaaa tcgaatggcg ctgggtacgc ggtcacaacg gccacccgga
     4321 aaacgaacgc gcggatcaac tcgccaatcg cggcgtcgcg tcgctcgcgg aaatatgatg
     4381 cgtacggtgc cgcccgcagc ggcactaccg cgcgcgccct tctttctttg ctgatttttt
     4441 tagagcaggc taatccgaca tgcgtcaact catcctcgat accgaaacca ccggcctgaa
     4501 tgcacgaacc ggcgaccgga tccttgaact cggttgtgtc gaaatggtga accgccggct
     4561 cacgggcaat aacctgcatt tctacatcaa cccggaacga gacagcgatc cgggcgcact
     4621 ggcggttcac gggctgacca ccgaattcct gagtgacaag ccaaagttcg ccgagatcgc
     4681 cgatcagttt cgcgacttca ttcaggacgc ggaactgatc attcacaatg caccgttcga
     4741 catcggcttt ctcgatgccg aatttgcgct gctgggtttg ccgccggtga agacctactg
     4801 cggcgagatc atcgatacct tggcgcgcgc caagcagatg ttcccaggca agcgcaattc
     4861 actcgacgca ctgtgcgacc gcttcggcat cagcaacgcg caccgcaccc tgcacggcgc
     4921 attgctcgac tcggaactgc tcgccgaggt gtatctggcg atgacacgcg gccaggaaag
     4981 cctcgttatc gacatgctcg gcgactcgca cggcggcggt gcacacgcgc cgcgcatcgc
     5041 gatcgattcc ctgaacctga tcgtgattgc cgcgagcgac gacgaggtcg ccgaacatca
     5101 gtcggtactc gacggcctcg ataaagcgat caaaggcacg agcgtgtggc gtctcgaacc
     5161 cgcgccggcc gcggatgagc aaaccgctta aaaagtactt tacgggtgca aaaatccctg
     5221 acataattcg gctctcttcg ggtggttagc tcagcggtag agcactgcct tcacacggca
     5281 ggggtcacag gttcaatccc tgtaccaccc accagattcg cgtgacccgc agaaataaag
     5341 gccctcagac gaaagtcgag ggcctttttg tttgcaccag aagtcacgaa ttctggctct
     5401 agggcggaga aggggcggag tggaaaatta gcccagacaa aacatgatga caatcaacgt
     5461 ctggagcatc tgatggcagc aatctggaag cgcggcaact actgaatgca tgcccgccaa
     5521 gggctggtga ccggaagctc gataattgaa tgaggatgag ctacgcaacc tcctggctgt
     5581 ctttgaaaat cgtgtattcg tcgttttcga tccggtagac attgacttgc tcatgccacc
     5641 ggcagaccgt cgttctcact ggacggggca tgttcgactt ccccgcgcgg aagaacgagt
     5701 tgaaagacgg cgccacgcgg cgcgtttgcg ctcgcccaga gtcgtcccct atgcgcctcg
     5761 acgatcgaac ggcagatcga cagtcccatg cccaagccat ctgccttcgt cgtatagaac
     5821 ggatcgaagg cacgctcgat cccatcttca ggaagtcccg gtccagagtc ccgtacggcg
     5881 atagacacgc cattcgatga atccgcggtc gtgctgatca gcaactcgcg cgggcccatg
     5941 ctcgtttcgc tcatcgcttc gatggcattg acgatcaggt tgagcatcac ctgttgcagc
     6001 tgcacgcgat ctcccaggac gagcggtaac ccatctccga gtactgtcag cactgaaacg
     6061 tcgttcttcg ccgcttcgcc ccgcgtcagt tcgatcacct cgcggaccgc ctcgttgatg
     6121 tctaccctgt ccttgcgtgg aggcgctttc ctgaccagat cgcgaatccc gctaacgatg
     6181 ccgccggcac gtatcgcctc attgacgatg agatcgagcg cctgcctgac ctccgcaagg
     6241 ttcgccgggt tggccgccag ccagcgtaag gcggccgaag cagaagttgc tgtcgcggcg
     6301 attggttgct gcacctcatg ggcgatcgag gccatgagct gtcccatcgt cgcagcccgg
     6361 tttgagtgct cgagctccgt ctgcacttcg cggtagcgcc gctcgctctc gcgcgcttca
     6421 gcttccgccc gcttgcgctc ggtcaggtcg agcacgaaac cgacaccttg gctcgcacct
     6481 tcttcgaatg tcgccgcgcc gaccagaacc ggaacgcggc tgctgtcctt cctggcgaat
     6541 tccttctcga agggctgtaa cgtccccgtc atccgaagct ccgggatccg ttgttgtatg
     6601 tcgcggtcaa gccactcggg cggtgtcagg tctgtccagc gcatccgccc cgacacgagg
     6661 tcctcgcggc cgtatcccac gatacgcaga aatgcgtcat tggcgtcgag gatttgacct
     6721 gccaggtccc agataaagat gccgacaatg ttggcgtcga cgaggcgccg gatcctcgct
     6781 tcacgttctg cgaggtcgcg gtacaggagc gcgttctcaa gagtgatcgc ggcctgcgaa
     6841 gcgagtagct tcagcaccgc gacgtgggtt ggcatgaaga cgcgtggcgt cagattgttt
     6901 tcgaggtaca gcatgccgac gagtccgccc tggttgatca gcgacaggca gagcgcggag
     6961 cggacttgat gttgctggat gtagggatcg gcgcaaaaca cgtcgtgttt tatcgcatca
     7021 tcgagaatga cgttctcgcg cgttcgcatg acgtagcgga gtatcgattc gggcagcatc
     7081 gccgtcgtca cgggctcatc acgcagctgt gtgacaaccg tgtcgcgctc gatggtggct
     7141 tctgccctga gccgcggctc ccccccgtgc gcgacgaaaa acaggagacc gcgcccggct
     7201 cccgcctgcg cgagcgcagt gcgcaacagc gtgtcgatca atttatccag gacgatctcg
     7261 cccgagaccg cctgcgagac cctgatcacg gtagccagat cgagatgttc gacgggcgcg
     7321 gcaatcgtgc tcgtcggacc gggtgccggc tcgtcctccc gcagctgcgg atgaagctga
     7381 tcgagttgcc gcaccttgcc ctcagcaccc cagcgcaggt agccctggcg ggccctgcgt
     7441 agatacgtcc cggcgaaact atccagacca cgcgccgcgt agaagcgcgc caccagttcg
     7501 aaagcgagcg acgcgttctg gacgaagccg ttgtcctggg ccgcgcggat ggcctgctcg
     7561 tagaaccgct cggcgtcgag ttcgcggccc tccaatcggg cgagttcggc gcggaccagc
     7621 gcggcgcggt cggagaagtt cgccgggcag tgctcggccc actcctggag ctggcgatgg
     7681 tgcgcggcca acgcgtccag gtgctctgcc cgctcttcga cggaggtcat gtcgcattgg
     7741 gccgcgtgcg caagggcgct atagaagtgg tattcggcca cctcgaaaaa cgacagcgac
     7801 gtccagagcc actgctccgc cttcgccgct gccgcgacag cgcacgcgtg ctccagggcg
     7861 aagtagcgag cctgcaactt gcggatccag tacctgcagg tcactaccgc cagacccggg
     7921 tcgccctgca gatgctgctc gaagcgatcc tcatcgaact ggtcgtcatt aaagcagccg
     7981 aacaccggcg tctggccgcg caacgtccgg atcagcccaa gctgtgcggt gacgagatcg
     8041 acgacgacac cgaactgcgc cttacgcacg aagccgagtc cggcctcagc ttcccgctgc
     8101 acctcgtcaa gcggatcgcc gccggcgagc atgaaggtga ccgtattgat gtagctgtaa
     8161 cccgtaaagg taaggtcacc agcttcctga gccatgtcga aggcgcgccg caacaagctg
     8221 cggcacgtgt tcaggtgccg cagccacgga ttcacaaggt agccgaagca catgtagaca
     8281 cgggccttga aacggtccag ccggcgcttc tcgaccagat cgatggctaa ctggccgaag
     8341 cggaacccgg cgcggtaatc gccgaagtgc ggccccagga tcatgcctag atagacatag
     8401 gcgaggcatg aggcgtcgct gttgccatac tgcaggctca ggttcgccat ccggcagaca
     8461 acgaggcaat agagatcgtt gtcagtccac gaggccggcg gcaggatctc ggcgaggacg
     8521 tccatgctgg cgcaagcggc cggatcgctc atcggcggca gttcgatgag cgcctcgatc
     8581 gagcgcttgc cgagctggcg ccagatgtgt tcgatctcct tacgcacctc gtcatgcgtc
     8641 ggatggggcg aacagtcgat acctacgcgg cgcagatact cgatccccac cgcgatggcg
     8701 tgtccgttct cacccgcggt catatagagc gatatgcgca ggcgggtcac ggcggccagg
     8761 tccacgaggg aagcggcatg ggtcggtagc gcagccaggc gcgcctcggc gccagtcaac
     8821 tggccggtca aaaactcgca ttcggcgtgg tggaattcaa gcgagaaggc gagtgcataa
     8881 cgccggtccc aacagtcctc ggccagcagt gcccgaccgg cagccaggta ggtcagtgcg
     8941 gagtgatagg ccatcgactg cttggcgcgc ttgcccgcca tgagattcaa ctccgcgact
     9001 cgctgacgct cctccacaga aatgagcaac ccggcacccc ggtttagctg gttcacgacc
     9061 tcgaagatgt tgtcctcgac cttctccggt gccgtccaca atccgagccg cctgccgatc
     9121 tgcagatgct gggtcggccg ctcacccgcg gggatcaggg cataggccgc ttcctgcacg
     9181 cggtcatgca ggaaagcgta ggcgcggtcc gagcgcaaga cgagcccgga ccggactgcg
     9241 tcccatagcg ccgcatggac cgccttttct gacaccccga gaaccgtgcc tagcgtggcc
     9301 gtttgcgcgc tggttccgag acaggcgagc cgtgtcagcg catcccgtgt ttcctccggc
     9361 aggcgggcga gcttgcccag cagcaacgtc accacgttgt cggtgtaacc cttcgcgcgg
     9421 atgcgctcaa ggtcccaggc ccaggtccgc cggcctggga cgaaggcgag tagtccctcc
     9481 tcccaaagga cggtaaggaa ctggatggca aagaacgggt tgccgccggt cttgacgtgc
     9541 accagccggg ccagcggcgc cgcgcgctgc ggcgcacact gcagcgcgtc ggcgatcaac
     9601 cggccgaggc ctgcgacgct gagcggcgtc aggaggatct cctgcacctg gcctcccgcg
     9661 ctgcggattg ccgcgagccg ccgcatcagc gggtgcgcgg gggtgacctc gttgtcgcgg
     9721 taagcgccga cgagcagcag gtggcgcagc tcccgttggg tcacgaggtg ctccagcaga
     9781 tcaagcgtcg ccaggtcgag ccactgcagg tcgtcgagga acagcgccag cgggtgctcc
     9841 gcccgcgcga acacaccgag cagccgacgg aataccagct ggaaacggcg ttgcgcttct
     9901 tgcggcggca gttccggcgc tggcggctgc ggaccgacga acagtcgaag ctcgggaatg
     9961 aggggcatca tcatctggcc gttcgggccc agcgcctcag cgagcgcctc gcgccacggc
    10021 gccagttcgg cgtcggtctg gcccagcagc gggcggatca gactctggag cgcctgcgcc
    10081 agggttgcgt aaggaatgtc gcgctgctgc agatcgaact tgccggatgc gaacaggccg
    10141 tgcggcgaca caaccgcccg gtgcagctct tgaaccaggg acgacttgcc gatgccggaa
    10201 tagccggaca ccagtaccag ctccggtccg ccgccggcca ccacccgttc gaaggcggca
    10261 agcagggtgt cgatttcgcg ctcgcgcccg tacagcgtct cgggaatcag cagccggtcc
    10321 gacgtgtcat cccggcccgg tgcaaaggta tggatggagc ctcgcgcttg ccattccgcg
    10381 agacagcggc gcagatcgct ctcgaccccg cccgcggtct ggtaacgctc ctcggccgtc
    10441 ttggcgagca gcttcatgac gatggcagag accacgtccg ggacgttctt cacccgttcg
    10501 ttcggcgcgt cgggcgtttt cgcgacgtgg cagtgcaccc actccgtcgg atcggtggcc
    10561 gtgaatggca acgagcccgt gagcatctgg tagagcgtga cgccaagtgc gtagaggtcg
    10621 ctgcgggcgt cgatggagcg gttcatccgc ccggtctgct cgggggccat gtaggcgaga
    10681 ctgccggcga tcagttcggg cggctcgggc gtctgccact cgcggggcag gcgcgacgca
    10741 atgccgaagc cggttagtcg gacctcttcg gtcgagccat tgacgaggat atgggatggt
    10801 ttgaggtctt tgtgtatcag gtcgcgctga tggacctggc cgacggcagt gacaatgcca
    10861 accgccacgc gcaagaagcg ccccatctcc agcggcgcgc caagttgcgc gtcgagcggt
    10921 tcaccctcga agtcttcgaa caggagtagc gttcgacggc cttcgcgcaa tagttccagc
    10981 ggccgcagcg cccatgcatt gtccagctct tccttcaggc cgtattcgtg ggcaagacga
    11041 tcgaggtccg cggcgctcga gcgctccgca gtgagccgca cggctagcac cacgtgctca
    11101 ttgccgtcgg catcgttgcg tcggacccga caccacgtgc gctcgccgtc ttcccttatg
    11161 acctgaaagt ggctatgtcc agacatatca agccgaaacg acgagctcaa cgttgccctc
    11221 cgcagtagtc cgagagccgc tccagccgct tgcagacgct ctgccagcgg acgacccgcc
    11281 cgagccgtta aatcagtctg ccacaaacgc ggcgaattga cgaacgcgct cccccgactt
    11341 ctggatacgg tagcggggcg agacgcagtc ctgtgcgaac catctgggtc agttccccga
    11401 ctttagagcg gcggacttac gcaccaccta cacaaaatta cgctgaacga acagatctgc
    11461 acagcggcgc gagctgtttt cgaagcccac agatgccgac gcggtgccag acgactccgg
    11521 caccggccac ttgcttcacg tcgtagggtc acgaagggtg ccaattcgcg accgcatact
    11581 atagcgaccc gcaccttgcg acgatggacc cgtcactctc tagtcgatcc gctgcggatc
    11641 gccgtcgaat tcgacgagac agccgaagcc gccgtgaaaa agcgcctcgc gcgcatcttc
    11701 cgcctttcct gtcgccgcga tctgcgccgc gtgcagttcg gcatcgtctt ctggctcgaa
    11761 cccgagaaaa cgcacgttgg cgttgtccca gcgattgcgc gcattagcag agacgccata
    11821 cacgaccgcg aagtggaagt ccggatagtc gatgcagcgg cgcgtcactt gcaccatgtc
    11881 gcgatggctg atccatgtga agaggtgccg cggcgcggtt ggctggtcat ccgggcggaa
    11941 cgagccgata cggatgcacg caaccgacat gccatgcttg tcggcgtaca tccggccaag
    12001 cgcctcgccg aacaccttcg aaatgccgta gcggctatcc gggcgcggct cgacggtgtt
    12061 atcgatcatc cggtcgcgac gatgaaaacc cactgcatga ttcgagctcg cgaacaccac
    12121 acgtttgacg cccgctcgac gcgccgcttc aaaaacgttg tagcagccgt cgacgttcat
    12181 gtcgcggatg cgctcccagg gagcctcgtc cggaacgcct gccagatgca cgacgacgtc
    12241 cacgccttcc ataaccggca gaagatcctc cagccgcgtg atgtccgcgg tcacgatttc
    12301 ctcgcgcgcc tgggcctcgg cttgcggcgc aatgtctgcg aggcgtaacg catagtggcc
    12361 ctggaaaccg gcgcgcagta cgcgtccgat gtgaccggcc gcgccggtaa tcagtactcg
    12421 agtcattgat accatccctg tcattcggta aaagtagtta atcggcttcg ctcttttggg
    12481 cggcgtagcc gaacgagcgg cggtgttgcg cttcaagaca ggcatcgcgc cacgacaccc
    12541 aatgctcccg gcgcagcccg accgacggtt cccggtcgcc ggtccgccgc gacccgatca
    12601 tctggtggcg ctccgcgcgc gcctgctcca agtactgcag aaacacgatc gataccgaac
    12661 agatggcatg cgtccggcgc tcatacccgt gaatcgcccg gtcaaaacct agctttcatg
    12721 cggcggtggt aggcgtctcg cctgcctcgc cggccgcgcg tcgaccgatg accagcagca
    12781 tggccagcac ggcgagcaga tcgaccgccg cgccgtgcac gaagccgctg ttgtaaccgc
    12841 caaagtactg gaccgcgata cccatcatgg tcagcccgac gatgcccgaa atgttgctca
    12901 tcaggtgaac aaagccactt acgcccccaa agatgcctgc ccggcacaag ctcgtgcatg
    12961 gtggcccagc acgcttgcga cgaagccgtc aggaacagca cggcgaacgc gacaagcagt
    13021 acggcgggcg ccacggagtc gaccatgctg acgcccagca gggcgacacc cgaaagcgcg
    13081 agcggcacga tcgcagcgac gttgcgcgcc agcaacttgt tgctcatgcg cttgaaaaat
    13141 gcgtcggcga ccagcccgcc gccgaagtat ccgatcccgc cgcatgccca cggcaacggc
    13201 accgcgctca gaatgctcat gtgctccatg tcaagatgca gctcgacggg ccagtagtcc
    13261 gcgccaaatg tgtagtcccg gtcgaactcg cggaactcga cgttatggct atggaccatc
    13321 gtgcctggcg caatatccgt cggcggcgaa accgatcacc acgttgtatt tgcgaaccgg
    13381 ctcgcccttt ttgattggca tcgccgcgat cttgcaacct gcggtcacct ggctgcggca
    13441 aacaaaatct tccaatggaa ctggcgtacc gattgcggcg tcgatgcgcg caattaccac
    13501 attgtcagcc ggatgcaacc aaatcactgg cccggttacg cttgctcgtc atctcagtca
    13561 tcaggcattg ccctttgaaa aatttgcgct cgacggtcaa aaagggagcg atacgagtaa
    13621 tcgctttctt aagtagaact gcagtcgacg cgtcgctgcg tgaaacgcat gaattttggg
    13681 catcttatgg cacttccgcg ttgaattcaa gagaaagcgc ttacttgatt ctctgcggtt
    13741 tccgagtagg taaggaggtc gcctctgtcg cgctatggcg gccatgtacg caatcgaatt
    13801 tgagatggcc tcgatggcca tgacgggcaa gacgccgcgc aagacattac cgccagggat
    13861 ggcgaaggtg ctgaagcggg ctgcactatc cgctggacgg tgcgacacgt gtcgccgtca
    13921 gccgcgcgtc aagcggcagg tagttgacgg taaagctcgt cctggtcttg tcgtacgcgg
    13981 cgatcggaaa cgattgcagt gctgcgacct gctgtgacgc tcctatgtca agtggcaagc
    14041 gtcgaatcgg ccagatcggc gaggatgagc gagtacagct ccctcacgat atagcgcttc
    14101 aggcagcggt gaatttcctt gttggacata ccttccttcg tccggcgctc gacgtaagcc
    14161 cgggtccggg gatcgcttct catgcggacc atcgcgatgg tccacaaggc attgtttgcg
    14221 gaccggtcgc cgcccctgtt caaacgatgc cggaccgtct tgccggacga cgactgcaac
    14281 ggacttgtgc cgcagagtgc agccagcgca gcttcgtttt tcaggcgttc gggattgtct
    14341 cccgccacag caaccaatac cgcagcggtc tgagggccga cgccgaatct ttcgcgaagg
    14401 cgtctggcat gctggctggt caggctttcg agcatgctat cgagagcttt gagctcctct
    14461 gccagcgtca gccaacgctt cgccagcaac cgcagcgtgg tcgtgagcgt ttgcaacatt
    14521 ggcgttttgc ccaacgagcg aagacgtgcg caaccctcaa cgcattctgc agtcttcgct
    14581 ttcagaagac gctcacggat ttcctgagga gcgctcacga gcaaagcccg taactgattg
    14641 atagcctgcg tcttggcctt gaccgcgctg cgcctcgcaa cacagacggc gcgcatcgct
    14701 tcggcagcgc cagactgttc ctttggaata gccgtagctc tgccggaaag aaccgcgcgc
    14761 gctgcgttct cagcatcagt gggatcggac ttgcctctgg accggcgcat cgcgcgatct
    14821 gggcgattga cttcaagcac ctcgatctca tgatcgcgca gcaggcgggc caatcccgct
    14881 ccataggtgc ccgtcccttc aaccccggcg cggcgtaaag gaccgaacga attcgcccat
    14941 gtcagcagat caagatatcc cgccgtgtct gtcgataccg atagcgttcc gagaagccta
    15001 cctgtgtcgc taatcacagc cccaacatgc gtatccaggt gcgtgtcgac acccagaatc
    15061 acttcgtttt gttccatgcg tatatccgtt gtagtgggcg catcgccaac cccactcgca
    15121 ggacaggaca ctcacggtgc agaacaaagc tcctatcagg tcacagacgg tgagcccggc
    15181 aacgctcggg gaacgccgga atccgatcga caggtcaacg caaaggcagc ttggccaatc
    15241 ccagcacggg tcagatcggt ccgacattca gaagcgatct tagactgagt gtcccaacaa
    15301 tcgccgcgtc ggctcattga cgagcgcatg ccgtaggcct tcagagcgtc ctgaaacagg
    15361 ccgccgcaat actgctgccc cggtcgctat gcacgattgc accagcctca gggcgacgcc
    15421 ggaaccacgc catgcgtagc gcgtcggtga ccagttcagc cttcatgtgc ggctgcatcg
    15481 accagcccac cacctgccga ctgaacaggt cgatgatgac cgcgaggtac acccagccct
    15541 cggctgtcgc cagatacgtg atgtcggtgg tccagacgtg gttcggtgcg tcggcggtga
    15601 agttgcgctc cagtaggtta ggagccaccg gcaggttgtg attcgagttc gtcgtcgcga
    15661 tgtacttgcg cttgtgccgg gcacggatgc cgtgctgagc catcagcttg cgaacgcgct
    15721 ccttgcccac gcgcacgccg cgtgcaacca gttccttcca catg
//
LOCUS       DATASH010000798         2487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1321330,
            whole genome shotgun sequence.
ACCESSION   DATASH010000798 DATASH010000000
VERSION     DATASH010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1321330"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(353..1348)
                     /locus_tag="VHV99_21475"
     CDS             complement(353..1348)
                     /locus_tag="VHV99_21475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3638001.1"
                     /translation="MASPTSSTAARDDAPRHVVVVVAFDRINPFHLSVPCVVFGEDRR
                     DGGLPNFDFRVCAAEGGILTTTAGFSIAVTHGLEALADADTIIVPSWRDPAETPPAAL
                     LDALRAANARGAQLVGLCLGSFVLAATGILDGRPASTHWAWADDFAHRYPRVRLDPDV
                     LYVDDGNVLTSAGTAAGLDCCLHVLRKMCGAQAANYVARRLVVPPHRQGGQAQYIQQP
                     VAPNVRGDRLSCLLEWVSGNLDAPHTLDTLAERALMSRRTFTRRFRLATGTTVGAWLL
                     AQRLARAQQLLESTDESVEAIAGIAGFGSTASLRQHFAEAFRTSPSAWRREFRGV"
     gene            1538..2170
                     /locus_tag="VHV99_21480"
     CDS             1538..2170
                     /locus_tag="VHV99_21480"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HEX3638002.1"
                     /translation="MTAPRRALIVIDVQNEYVTGDLPIEYPDVQTSLANIGRAMDAAR
                     TAGVPVVVVQNFAPATSLLFARGSVGAELHPVVGSRPHDHYVEKSLPSAYAGTDLGAW
                     LAAREIDTLTVIGYMTHNCDASTVFHALHDGLAVEFLADATGSVPYENSAGFASAQDI
                     HRVFSVVLQSRFAAVASTEQWIAAVKSGAPLERSNIYASNQKARANQAAA"
BASE COUNT          481 a          830 c          801 g          375 t
ORIGIN      
        1 ctttgttgag ttggtgacgg cgacattagg gcaccggtaa cattggggcc accgccaccg
       61 ccaccgccac cgccaccgcc accgccaccg ccaccgccaa agccaaagcc aaagccaaag
      121 ccaaagccaa agccaaagcc aaaggccact gccaagcgca cctctgtcgc gctgacgcga
      181 ctacgaaatc ctggtgggtc actacgaaac gccgtcgact aggtggcgtt tgcggccctc
      241 gaagggctta tccggtctcg cttgtttcag ccccgatcgc cttgatccgc tcccacgcat
      301 gctcgccttt ccccgctctc aaacaaaaaa tatcgcgcgc cttcacacgc gctcacacgc
      361 cgcgaaactc ccgccgccat gcggacggcg aagttcggaa cgcctccgcg aaatgttgcc
      421 gtagcgaagc ggtcgagccg aaacccgcaa tccccgcgat cgcttccact gactcatccg
      481 tgctttccag caactgctga gcgcgggcga gccgctgcgc cagcagccat gcgccgacgg
      541 tcgtgccggt ggccagccgg aagcgccgcg tgaacgtgcg gcggctcatc aacgcgcgct
      601 cggcgagcgt atcgagcgta tgcggcgcat ccagattccc gctcacccac tcgagcagac
      661 aggacaggcg gtcgccgcgc acgttcggcg ccaccggttg ctgaatgtac tgcgcctggc
      721 caccctgccg atgcggcggc accaccaggc ggcgcgcgac atagttcgcc gcctgcgcgc
      781 cgcacatctt gcgcagcaca tgcaggcagc agtcgagacc ggcggcagtg ccggccgagg
      841 tgagcacgtt gccgtcgtcg acatagagca cgtccgggtc gagccgcacg cgcgggtagc
      901 ggtgcgcgaa gtcgtccgcc cacgcccaat gggtcgacgc cggccggccg tcgagaatac
      961 cggtggcggc cagcacgaac gagcccaggc acagcccgac cagctgggcg ccacgcgcat
     1021 tcgcggcgcg cagcgcgtcg agcagcgcgg cgggcggcgt ctcggccgga tcgcgccagc
     1081 tcggcacgat gatcgtgtcc gcgtcggcga gtgcttcgag cccatgcgtg acggcgatcg
     1141 agaagccggc ggtggtcgtc aggatgccgc cctcggccgc gcagacgcga aagtcgaaat
     1201 tgggcaaacc gccgtcacga cggtcctcgc cgaacaccac gcacggtacc gaaagatgga
     1261 acgggttgat gcggtcgaac gcgacgacca caacgacgtg gcggggcgca tcgtcgcgcg
     1321 ccgcggtgga ggaggtgggg gaagccatca gcgcagtcgt cctgtgaaat ccaacgatga
     1381 tccaacgatg gcccgattct agcgaatctt gtcgttcggg tcgctatcgg gaaaacggcg
     1441 agaggccaac aatgcagtca tcgccgctcc ctgctcgggg cccatgccgg gccgcgcagc
     1501 gcgagcccca accgattaac ccacaaggag tctggccatg accgcaccac gccgcgcgct
     1561 gatcgtcatt gatgtgcaga acgaatacgt caccggcgat ctgccgatcg aatatccgga
     1621 tgtgcaaacc tcgctcgcca acatcggccg ggcgatggat gcggcccgca cagccggcgt
     1681 gccggtggtg gtcgtgcaga atttcgcgcc ggccacctca ctgttgttcg cgcgcggcag
     1741 cgtcggcgcg gaactgcacc cggtggtggg ctcgcgcccg cacgatcact atgtggagaa
     1801 gtcgctgccg agcgcttacg ccggcaccga tctgggcgcc tggcttgccg cgcgagagat
     1861 cgacacgctg acggtgatcg gctacatgac ccataactgc gatgcgtcga ccgtattcca
     1921 cgcactgcac gacgggcttg cggtcgaatt cctggccgat gcgacgggct cggtgcccta
     1981 cgagaacagc gcgggttttg cgagtgcgca ggacattcac cgggtgttca gcgtggtgtt
     2041 gcagtcgcgc ttcgcggccg tggcgagcac ggaacaatgg attgcggcgg tgaagagcgg
     2101 agcgccgctc gaacgcagca acatctacgc gtcgaaccag aaggcgcggg cgaatcaggc
     2161 ggccgcctga ggctgctgcc tgaggctgcc gccagagcat gctgcaaggg gctcggtgaa
     2221 aggcctgctg caaagatctg ctgcaaaggc ctgcggtaag aacatgctgt taagacccgc
     2281 tgcaaaggcc tgcggtaaga acacgctgtt aagatccgct gcaaaggtct gcggtaagaa
     2341 cacgttgtta agacccgctg taagaccggc ggcagtagtg cctcttgtaa acgcctgcac
     2401 gcgcaaacaa aaaaaagcgg gcgggttgcg cgaatgcgca ccccgcccgc tgtttcgtgg
     2461 ccgcgctaat tattcagcta gcggacc
//
LOCUS       DATASH010000799         1801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1915421,
            whole genome shotgun sequence.
ACCESSION   DATASH010000799 DATASH010000000
VERSION     DATASH010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1915421"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /gene="aroA"
                     /locus_tag="VHV99_21485"
     CDS             complement(<1..882)
                     /gene="aroA"
                     /locus_tag="VHV99_21485"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009921655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX3638003.1"
                     /translation="MEHLDLGPFARASGTVRLPGSKSISNRVLLLAALAEGETVITNL
                     LDSDDTRVMLAALNTLGVEVQNNGERCVVKGTGGRFPVKSAELFLGNAGTAVRPLTAA
                     LALNGGEYRVHGVPRMHERPIGDLVDGLRQIGAKIDYEANEGFPPLRIHPAEIAVDQP
                     IRVRGDVSSQFLTALLMSLPLAQNTRGPIVVEVDGELISKPYIDITIKLMERFGMTVE
                     RDGWKSFTLPAGARYASPGTIAVEGDASSASYFLAAGALGGGPLRVEGVGRASIQGDV
                     NFADALIRMGANVMLGDD"
     gene            complement(935..>1801)
                     /locus_tag="VHV99_21490"
     CDS             complement(935..>1801)
                     /locus_tag="VHV99_21490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006750756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEX3638004.1"
                     /translation="RALRERAGGAGAAGEVVGVGRSVKSVERALELGVIDTAVFLDDD
                     RALELALRDADFVFIAAPVAQTEPLLRRIAPFLDARTIVTDAGSTKNDVVVAARAALG
                     DRVAQFVPGHPIAGRESSGPDAAIPDLYVDRNVVLCPLPENASAAVERVADMWLATGA
                     SVHRMTAEQHDSVFASVSHLPHVLSFALVEMILQAPDAALKFSFAAGGFRDFTRIAAS
                     SPEMWRDVCIANRAALLTEIDAYTNVLARFREAIATSDGAALETAFERSRTARTEWQQ
                     GGAIKAADESAQ"
BASE COUNT          354 a          606 c          534 g          307 t
ORIGIN      
        1 gtcatcgccc agcatgacat tcgcgcccat gcggatcagc gcatcggcga aattcacgtc
       61 accctgaatg ctcgcgcggc ccacgccttc cacgcgcaac ggaccaccgc ccagcgcgcc
      121 ggccgcgagg aaatacgacg ccgacgacgc atcgccttcc accgcaatcg tgcccggcga
      181 cgcgtagcgc gcgccggccg gcagcgtaaa actcttccag ccgtcacgtt cgacagtcat
      241 cccgaaacgc tccatcagct tgatcgtgat gtcgatatac ggcttcgaaa tcagctcacc
      301 gtccacttcc acgacaatcg ggccacgcgt gttctgcgcg agcggcaagc tcatcagcaa
      361 cgcggtcagg aactggctgg agacatcgcc acgcacgcgg atcggctggt cgacggcgat
      421 ttcagccgga tgaattcgca acggcggaaa accttcattc gcttcgtagt cgatcttcgc
      481 gccaatctgg cgcaagccat cgacgagatc gccgatcggc cgttcgtgca tgcgtggcac
      541 gccatgcacg cgatattcac cgccgttcag cgcaagcgca gccgtgagcg gacgcactgc
      601 agtaccggca ttgccgagaa aaagctccgc cgatttcacc ggaaagcggc caccggtccc
      661 cttcacgaca cagcgctcgc cattgttttg cacctcgacg cccaatgtgt tgagcgccgc
      721 gagcatcacg cgggtgtcgt cggaatcgag caggttcgtg atcaccgttt cgccttcagc
      781 cagcgcagcg agcagcaaga cgcggttaga aatgctcttc gatccgggta gccgaaccgt
      841 gccggacgca cgcgcaaaag gtccgagatc gaggtgttcc atgattaagt ccagttatct
      901 aatgtgcgga ttattgtgca gcttactgtg cggattattg ggccgactcg tcggcggctt
      961 tgatcgcgcc gccttgttgc cattcggttc gcgctgtgcg cgagcgttcg aaggcagttt
     1021 cgagcgcggc accgtcggag gtagcgatag cctcgcgaaa acgcgccagc acgttcgtat
     1081 aggcatcaat ttcggtcagc aacgccgccc ggttcgcgat gcacacgtcg cgccacattt
     1141 ccggactcga cgccgcgatc cgcgtgaaat cgcggaagcc gccggcggcg aacgaaaact
     1201 tcagcgcggc atcgggcgct tgcagaatca tctcgacaag cgcgaacgac aacacgtgcg
     1261 gcaaatggct caccgatgca aacacgctgt cgtgctgctc ggccgtcatc cgatgcacgc
     1321 tggcgcccgt cgcaagccac atgtcggcga cgcgctctac ggccgccgat gcgttctccg
     1381 gcagcgggca cagcacgacg ttccgatcca catagagatc gggtatggcg gcatcaggac
     1441 cactcgattc acggccggcg atcggatgcc ccggcacgaa ttgcgcgacg cgatcgccga
     1501 gcgctgcacg cgccgcgacg acaacgtcat tttttgtgct gccggcatcg gtgacgatgg
     1561 tgcgcgcgtc aaggaacggc gcaatccgcc tcagcaacgg ctcggtttgc gccaccggcg
     1621 cggcgataaa cacgaagtcg gcgtcacgca gcgcgagttc gagcgcgcgg tcatcgtcca
     1681 gaaaaacggc cgtatcgatc acgccgagtt ccagcgcacg ctcgacggac ttcaccgagc
     1741 gcccgacgcc gacgacttcg cccgctgcgc ctgctccgcc agcgcgttcg cgcaatgcgc
     1801 g
//
LOCUS       DATASH010000800         2559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4880541,
            whole genome shotgun sequence.
ACCESSION   DATASH010000800 DATASH010000000
VERSION     DATASH010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4880541"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1079)
                     /locus_tag="VHV99_21495"
     CDS             complement(<1..1079)
                     /locus_tag="VHV99_21495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HEX3638005.1"
                     /translation="MSEAARFTELSDLAPATESLREIRHHIHHHPELAYEEVQTGALV
                     AQKLEQWGWQVTRGVGQTGVVGSLKVGDGTRSIGIRADMDALPIIEQTGLPYASGTHG
                     KMHACGHDGHTTMLLGAAQHLAATRNFSGTVHLYFQPAEESGIDSGAMKMINDGLFER
                     FPCDAVFGLHNHPGEEPGVMLFRKGPFMSAGDKAIITIEGVGGHAARPHLTVDPVVVA
                     ASIVMALQTIVARNVDPSQPAVVTVGSMHAGTANNVISSSARLELSVRSFSPDVRALL
                     KKRITELAESQAASYGGKAVVEYIEGYPVVINSDAETDFAVQVARELVGDDKVVEQTD
                     ILMGSEDFAFMLQKRPGTFLRIGNGA"
     gene            complement(1297..1734)
                     /locus_tag="VHV99_21500"
     CDS             complement(1297..1734)
                     /locus_tag="VHV99_21500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638006.1"
                     /translation="MIKRLTFFALIGVTFQAHACNVTYSTASDSVDKAITEQGWDFKN
                     YDIICEKLRAANAALLVDGQATVLGGKSIAWASVKLREKNLTIFTADYGGKSTQTNDY
                     ASIDKADSMLITAINRAVESVDVDKAIKSLAEQRSLVKAAYSK"
     gene            complement(2043..>2559)
                     /gene="phnN"
                     /locus_tag="VHV99_21505"
     CDS             complement(2043..>2559)
                     /gene="phnN"
                     /locus_tag="VHV99_21505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphonate metabolism
                     protein/1,5-bisphosphokinase (PRPP-forming) PhnN"
                     /protein_id="HEX3638007.1"
                     /translation="AGKDSLLSFARSRLMGEPIVFAHRYITRPSGNGEEHIALSVEEF
                     ATRSVLGLFALEWSSHSLRYGIGIELDAWLTRGCTVVVNGSRQHLQHTLTRYRHTEVV
                     HVDAAPHILAARLGARARESAEQVAARLARRAPFSLPEGLRCTTIDNSGALEDAGHAL
                     IAFLQAEADVQ"
BASE COUNT          515 a          814 c          699 g          531 t
ORIGIN      
        1 gcaccgttgc cgatgcgcag gaacgtaccg ggccgcttct gcagcatgaa agcgaaatct
       61 tcgctgccca tcaggatgtc ggtctgctcg accaccttgt cgtcgccaac cagttcgcgc
      121 gccacttgta ccgcgaaatc cgtttcggca tccgaattga tcaccaccgg ataaccttcg
      181 atgtactcca ccaccgcctt gccgccatag ctcgcggcct ggctctccgc gagctcggta
      241 atgcgctttt taagcagcgc gcgcacgtcg gggctgaacg accgcacgct cagttccagc
      301 ctcgcgctgc tggagatcac attgttcgcg gtgcccgcat gcatcgagcc gacagtcacc
      361 accgccggct gcgacggatc gacgttacgc gcgacgatcg tctgcaacgc catcacgatg
      421 ctcgccgcca ccaccaccgg atcgaccgtc agatgcggac gggccgcatg accgccgacg
      481 ccttcgatcg tgataatcgc cttgtcgccc gccgacatga acggcccctt gcggaacagc
      541 atcacgcccg gctcctcgcc cggatgattg tgcagaccga acacagcgtc gcacggaaag
      601 cgctcgaaca ggccgtcgtt gatcatcttc atcgcgccgc tgtcgatgcc gctttcttcg
      661 gccggctgga agtacagatg cacggtgccc gaaaaattgc gcgtggcggc gaggtgttgc
      721 gcggcgccga gcagcatcgt tgtgtggccg tcgtggccgc atgcgtgcat cttgccgtgc
      781 gtgccgctcg cgtaaggcag accggtttgc tcgatgatcg gcagcgcgtc catgtcggcg
      841 cgaatgccaa tgctgcgcgt gccgtcaccc accttcaagg aacccaccac gccggtttga
      901 ccgacgccac gcgtgacctg ccagccccat tgttcgagct tctgcgccac caatgcgccg
      961 gtttgcactt cctcgtaggc gagctccgga tggtgatgga tgtgatggcg aatctcacgc
     1021 aggctctcgg ttgcaggcgc caggtcggac agttcggtaa aacgcgcggc ttcgctcatc
     1081 tatcgctcca tgagaattca cgccgcatca cgcggcaaac aggctagccc tatagctacg
     1141 ccgtcttgtt cgaagccggc cggggtcgac accggccagc aaagatccca ttgtgacaaa
     1201 gactgaattg ttccgcgact cttcgccgac gaagaatcca gtcggcgcga tcgtagacgt
     1261 ggaacactgc ggcctgaact ggccgaattg actggcttac tttgagtaag cggcttttac
     1321 caggctacgt tgttcggcca gggatttaat agccttatca acgtcaacgc tttcaacggc
     1381 cctgttgata gcagttatca acatcgaatc cgccttgtcg atgcttgcat agtcatttgt
     1441 ttgcgttgat tttccgccat agtccgcggt aaaaatcgtc agattctttt ccctcagctt
     1501 gaccgaggcc catgcaatcg acttgccgcc caaaaccgta gcctgcccgt caaccagtaa
     1561 cgcagcgttg gccgctctca atttttcgca gatgatgtcg taatttttaa aatcccatcc
     1621 ctgttccgtg atggctttat caacactgtc ggatgccgtg ctgtaagtaa cattgcacgc
     1681 gtgcgcctgg aaagtcacgc caatcaaggc gaaaaacgta agtcgcttaa tcatcaaact
     1741 cactcaagat aagaatgtag atattgaatt tgcgctttct gcgtggccaa gcgaattgca
     1801 ctggccgggc cggctggccc attcaatcgg ctagccgtgg ccctcgaggc cgtagcaata
     1861 gccgtaggcc tgttacccgc atgtgcacaa atccgcctcc gcctttctta tcggattcag
     1921 tttgaaagac ttgagggtta aatgaaaatt tattccaagc gccgagaaat caaggcgcgc
     1981 cccggtctgg tcgtcgtgcg gcgcaccgcg ttcgaggtgc gccgacacct gtggcagcca
     2041 ctttattgaa cgtcggcttc ggcctgcaag aaagcaatca gtgcgtgtcc cgcgtcttcc
     2101 agcgccccgg aattgtcgat cgtcgtgcac cgaagtcctt ccggcaatga aaacggtgcc
     2161 cgccgcgcga gcctggccgc gacctgttcc gccgactcac gcgcccgcgc gccaagccgt
     2221 gctgcgagaa tatgcggcgc ggcgtcgaca tgaacgacct ccgtatgccg ataacgcgtc
     2281 aacgtatgct gcaaatgctg acgcgatccg ttgacgacaa ccgtgcaacc gcgtgtaagc
     2341 catgcgtcga gctcgatacc aatgccatag cgcagcgagt ggctcgacca ttcgagcgcg
     2401 aacagaccga gcacggaacg cgtcgcgaat tcttcgacgc tcagcgcaat atgctcttca
     2461 ccgttgccgc tcggccgcgt gatgtagcga tgcgcaaaca caatcggctc gcccatcagg
     2521 cggcttcgcg caaaactcag caaggaatcc ttgcccgcg
//
LOCUS       DATASH010000801         3807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3693713,
            whole genome shotgun sequence.
ACCESSION   DATASH010000801 DATASH010000000
VERSION     DATASH010000801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3693713"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..1846)
                     /locus_tag="VHV99_21510"
     CDS             complement(62..1846)
                     /locus_tag="VHV99_21510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy polymerase domain-containing protein"
                     /protein_id="HEX3638008.1"
                     /translation="MPTPFTRYLSLILLSLALVLPYAVVNHTYPIPTFYAEFTALALY
                     LLLGAGVVLLVWTARPRVTFASPVVALVPLLFGLVLVAQSVLLPVSQPSMNWLGGGYL
                     LAAFMATHAGYGFTRVKLNETALRWAAGALIVGGLFAVFCQVIQLFHLEARVTPLVVA
                     YNNTVERRPFGNMAQANHLATYIAFAMAGALYLVQTRRLAVSIWLLVSTIFAVGLALT
                     VSRGPWLQMGVIVVAGFWMAFAQMRSQSHLRRSNREWLIPIALTVLFFVVNALVRWAN
                     VHYHLELGQSAAERFKDAGQIAPRLALWKYGWTMFKAHPLLGVGWGEFPSYQFEYAKS
                     LGGVEIANNSHDIFIDLLAKTGLIGLAIVLFGLITWLVRVVRAPQSAARVFGVALIGV
                     LVMHALVEYPQQYMFFLLPAMFVFGLLETRPLRLVPGRLSFGVFAAIVFGGLAALYPV
                     FRDYARAEVLYYGSRPAEQYRAAPSFLFQAWGEYGLATLLPMNSMDLQHKLAMHKQAI
                     ALLPGETVLRRYAVLQALNGDTAGAFDTVERLKIFAQELKDWPSQLSYLYELCDEQKT
                     LTGFKAELVKRYGTPPGDVNEDDDSGED"
     gene            complement(1983..2606)
                     /locus_tag="VHV99_21515"
     CDS             complement(1983..2606)
                     /locus_tag="VHV99_21515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEX3638009.1"
                     /translation="MIVTLPYSPFSLSAVSPRSAFERARWTAPLVRACRCFGRGFTLI
                     ELMIVLAIVGVIAAYAIPAYQDYLARSRVGEGLSLAASARLAVSENAASGNAFGGGYA
                     SPPATRNVESIHVDDDTGQITIAFTTRVAPAGSNTLTMVPSVPDNADAPTARIALSKG
                     AVQAGAVTWECFAGGKTASSLPAPGAGPAPADAPSLASNLAPPECRA"
     gene            complement(2736..3440)
                     /locus_tag="VHV99_21520"
     CDS             complement(2736..3440)
                     /locus_tag="VHV99_21520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX3638010.1"
                     /translation="MLEFFATLHWGAVIQIIVIDILLGGDNAVVIALACRNLPAEQRT
                     KGVLWGTAGAILLRVALIAFAVALLDVPLLKFAGGLLLLWIGVRLMAPAHDVHENIKP
                     ADKLMAAIKTIIIADAVMSLDNVIAIAGAAEAADPQHRLALVIFGLVVSIPLIVWGSQ
                     LVLKLLDRFPIVITLGAALLGWIAGGLIINDPAGDRWPILDTPAAEYGMCIAGALFVV
                     ILGYLLKRRNANRAAA"
     gene            complement(3589..>3807)
                     /locus_tag="VHV99_21525"
     CDS             complement(3589..>3807)
                     /locus_tag="VHV99_21525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX3638011.1"
                     /translation="NAAEWIKDNMKKPVVGFIAGVTAPPGKRMGHAGALISGGADTAE
                     AKLEIMEACGITVTKNPSEMARLLKAML"
BASE COUNT          851 a         1247 c         1135 g          574 t
ORIGIN      
        1 acgtcatcgc cccgctccac ctgtaaagct gcggctaagg cggcggctaa aacacagcgg
       61 ctcaatcctc gccgctgtcg tcgtcctcat tgacgtcgcc aggcggcgtg ccgtagcgct
      121 tgaccagttc cgccttgaaa ccggtcagcg tcttctgctc atcgcacagc tcatacaggt
      181 aggacaactg cgacggccag tccttcagct cctgagcaaa gattttcagg cgctcgacag
      241 tgtcgaacgc ccccgccgtg tcaccgttca atgcctgcag cacggcgtac cggcgcagca
      301 cggtttcacc cggcagcagc gcgatcgcct gtttgtgcat ggcgagcttg tgctgcagat
      361 ccatcgaatt catcggcagc aacgtcgcga ggccgtactc gccccacgcc tggaacagga
      421 acgacggggc cgcgcgatac tgctcggcag gacgtgagcc gtagtacagc acctcggcgc
      481 gcgcatagtc gcgaaacacc ggatacagcg cggccaggcc gccgaacacg atcgcagcaa
      541 acacaccgaa cgacaagcgg ccaggcacaa ggcgcagcgg ccgtgtctcg agcaagccga
      601 acacgaacat cgccggcagc aggaaaaaca tgtactgctg cggatactcg accaacgcat
      661 gcatgaccag cacgccgatc aacgcgacgc cgaacacgcg tgcggcgctc tgcggcgcgc
      721 ggaccacgcg caccagccac gtgatgaggc cgaacagcac gatcgcgagg ccgatcaagc
      781 cggttttcgc gagcagatcg ataaagatgt cgtgcgagtt attggcaatc tcgacgccgc
      841 ccagcgactt cgcatactcg aactggtagc tcggaaactc gccccagccg acacccagca
      901 gcggatgcgc cttgaacatc gtccagccgt acttccacag cgcaaggcgc ggcgcgatct
      961 ggccagcgtc cttgaagcgc tcggccgcgg actgcccaag ctccaggtga tagtgcacat
     1021 tggcccagcg gaccaacgcg ttgacgacaa agaacagcac cgtcaatgcg atcgggatca
     1081 gccattcgcg attgctgcgg cgaaggtgag actgactgcg catctgcgcg aaagccatcc
     1141 agaaacccgc caccacaatc acgcccatct gcaaccaggg tccgcgcgac acggtcagcg
     1201 cgagtccaac ggcgaaaatc gtcgacacga gcaaccagat gctgacggca agccggcgtg
     1261 tctgcacgag gtacagcgcc cctgccatcg cgaacgcgat ataggtggcc aggtgattcg
     1321 cctgcgccat attgccgaac ggccggcgct caacggtatt gttataggcg acgaccagcg
     1381 gcgtcacgcg cgcctcgagg tgaaatagtt ggatcacctg acagaacacc gcaaacaggc
     1441 caccgacgat caacgccccc gccgcccagc gcagcgctgt ctcgttcaac ttgacgcgtg
     1501 tgaacccgta gccggcgtgc gtcgccatga atgcggcgag cagatagccg ccgccgagcc
     1561 agttcatcga cggctgcgag accggcagca gcacggattg cgccaccagc accagcccga
     1621 acagcaacgg caccagcgcg acaaccggcg acgcaaacgt cacgcgcggc cttgccgtcc
     1681 acaccagcag cacgacaccc gcgcccaaca gcagatacag cgcgagcgcc gtgaattcag
     1741 cgtagaaagt cggaatcgga tacgtatggt tgacgactgc ataaggcagc accaacgcga
     1801 gagacagcag gatgagagac aggtagcgcg taaatggggt gggcattgaa taaccgatgg
     1861 gcgtcagcaa ccggcgcact atacaaaaaa attctcctgc tgtgcgccgg ccgggctgaa
     1921 atatcccgag aatcagtgtt ttacgtccgg attacgactc acccgctcgt gcgccccact
     1981 gctcaagcgc ggcactccgg tggtgcaaga ttcgacgcca acgacggcgc atcggcaggt
     2041 gcgggaccgg cgcccggcgc aggcaacgag gaggccgttt tgccgccggc gaaacattcc
     2101 cacgtgaccg cgcccgcctg cactgcgccc ttgctcaacg cgattcgcgc tgtgggcgcg
     2161 tcggcattgt cgggcaccga cggcaccatg gtcagcgtgt tcgaaccagc aggcgcgacg
     2221 cgcgtcgtga acgcgatggt gatctggccg gtgtcgtcgt cgacgtgaat ggactcgacg
     2281 ttgcgggtag ccggcggcga tgcatacccg ccgccgaacg cattgccgct agcggcgttt
     2341 tcagacaccg cgagccgcgc ggacgccgcc aacgagagac cctcgcccac ccggctgcgg
     2401 gccagatagt cctgatacgc ggggatcgca taggccgcga tcacgccgac aatcgccagc
     2461 acgatcatca gctcgatcag cgtgaaacca cgcccaaaac agcggcaggc acgtacgagg
     2521 ggcgcggtcc agcgcgctcg ttcgaacgcg ctccgggggg aaaccgcgga cagtgaaaaa
     2581 ggggaatacg gcaaagtaac gatcattggc aggctcctga aacgaaaaac gcctgcccct
     2641 tgaagtgggg acaggcgctt caatcgtagc cagcggtact cgttccacac agtcagccga
     2701 atggctaacg ttgcgatgac gcggcggtgt tcaggtcaag ccgccgcgcg attcgcgttg
     2761 cggcgcttga gcagataacc caggatcacg acgaacagcg caccggcgat acacatgccg
     2821 tactccgccg ccggtgtatc gagaatcggc caccggtcgc cggccgggtc gttgatgatc
     2881 aggccgcccg cgatccagcc gagcagcgcg gcgccgagcg tgatgacgat cggaaaacgg
     2941 tcgagcagct tcagcaccag ctggctgccc cacacgatca gcgggatgct caccaccagg
     3001 ccgaaaatca ccagcgccag gcgatgttgg gggtcggccg cctcggctgc accggcaatc
     3061 gcgatcacat tgtccaggct catgaccgca tcggcgatga tgatggtctt gatcgccgcc
     3121 atgagtttgt cggccggttt gatgttctcg tggacgtcat gcgcgggcgc catcagacgt
     3181 acaccgatcc acagcagcaa caggccgcca gcaaatttca gcaacggcac gtcgagcagc
     3241 gccacggcaa acgcaatcag cgccacgcgc agcaaaatcg cgccggcggt gccccacagc
     3301 acgcccttgg tacgttgctc agccggcagg ttgcggcagg caagcgcgat cacgacagca
     3361 ttgtcgccac ccagcaggat gtcgatgacg atgatctgaa tgacggcgcc ccaatggagc
     3421 gtggcgaaga actcgagcat ggctaaaaga ataagaagga atcagacgca cagccaataa
     3481 aaaaagcgag ggagtctaaa aactcccccg ctttttaaga ctgcccgctc acgaaaggat
     3541 ttcgtaagcg tatcaaaaaa cgcgccgtga ctgcacgcga atttcgattt acagcatcgc
     3601 cttcagaaga cgcgccattt ccgacgggtt cttcgtaacc gtgatgccgc aggcttccat
     3661 gatttccagc ttggcttcag ccgtatccgc accgcccgag atcagcgcgc cggcgtggcc
     3721 catgcgcttg cccggaggcg ccgtgacacc ggcgataaag ccaaccaccg gcttcttcat
     3781 gttgtccttg atccactcag cagcgtt
//
LOCUS       DATASH010000802        13076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6661540,
            whole genome shotgun sequence.
ACCESSION   DATASH010000802 DATASH010000000
VERSION     DATASH010000802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 13076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13076
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6661540"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..133)
                     /locus_tag="VHV99_21530"
     CDS             complement(<1..133)
                     /locus_tag="VHV99_21530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpB/DapX lipoprotein domain protein"
                     /protein_id="HEX3638012.1"
                     /translation="MTDLRLTKRFAVMLMAGGLVAGCSTSSPTKIDYKSDSKSKQVSL
                     "
     gene            594..1070
                     /locus_tag="VHV99_21535"
     CDS             594..1070
                     /locus_tag="VHV99_21535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2844 domain-containing protein"
                     /protein_id="HEX3638013.1"
                     /translation="MPNPCFSPALSPVIAASAIVAALLCVSADARAELGGTMPSHADS
                     TAAAPQTLLNGALRMRTLTDAGNTTINEYATNTGQIIAYTWQGPTMPDLRALLGKYAD
                     SYRTGATASAPDGNLHASRVARPDVIVESGGPMRGYAGRAWLPAALPAGVSAADFR"
     gene            1125..2402
                     /locus_tag="VHV99_21540"
     CDS             1125..2402
                     /locus_tag="VHV99_21540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3443 domain-containing protein"
                     /protein_id="HEX3638014.1"
                     /translation="MKTPSRFHALLLVCAALVTGCGGGGDTASSQAGAASPASSPGAA
                     GSSGVSAPSAGGLSSTATSVPQSTTPNVQPITVGAVPGLTRNILTTSVTVCQPGTSSC
                     ATIDNIQVDTGSHGLRILASALPSSLTLAAVGSGSGVTGECSVFGSGYTWGSVRSADV
                     RMAGQLAASLPIQLIADPTLPTVPSDCASSGPSMLTGSKPYANGILGVGLFAADCGSG
                     CVNSALPRWYYNCDGSGACLASTQALAQQVTNPVSRFALDNNGVVIDLPAIADAGAAS
                     VSGSMIFGIGTQANNTLAGASVVKANQVTGWVVTSSAGQTYAQSYIDSGSNGLFFSSS
                     QFPQCGYWYCPSSTQTANASITGTDGVSTTVSYSIGNSRALFASTNNAFNNLAGVVSN
                     GFGWGLPFFFGRRVYTAIASRATSAGPGPYYAF"
     gene            2702..4573
                     /locus_tag="VHV99_21545"
     CDS             2702..4573
                     /locus_tag="VHV99_21545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006052538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HEX3638015.1"
                     /translation="MELTFSLDHFEQLVYTQQHAQASVQLIAALALLQHTRGELDANF
                     QASGMTDLPPQGQRQRFCTRLTSAVSTLFADPDFRPPTECFRLFLTLHEWIGVLFSAT
                     ASGNADHVARYLNPRGPADAQFLPGDSFIEKLCVLYSTESELELDFAALWAYDKTIAA
                     CLALVLLAPVFKGSPSAHLKREALLEWLPGRLQQIDDLDDLPSAVLHNAYMFCSYADT
                     PRRHAVKQDINVLVRRKLAQLGLTDLPASKRTPVKSKSRRGKKPVMAVVLEWFSGAHS
                     IYRTHSRTLEAAREHFEVVGFGFGYAVDDVGRKIFDRFIELEQPEYIGECLKTIRDFA
                     EAEQPDVLYMPSVGMFVLTVFVSNLRVAPLQIAALGHPATTHSNKIDYISVEEDYVGD
                     PACFSEQLMKLPKDGQPYRPSVALPDIVARIPPPRETLQVVITASAMKLNPGFLDACR
                     AIGQRAATPVEFHFMSGVPSGLPFDRLRDVIAQALPGAVVHDFHDYAAYLARVNQSDL
                     FLSPFPFGNTNGIVDALTLGLPGVCKRGPEVFERIDGAQFERVGMPSWSTTDSVEAYI
                     TAAVRMIDQHDERTALRQRLIDTQAVERCFEGRPEAFGESVLKLLRKNKNRHVNGAA"
     gene            complement(4792..5361)
                     /locus_tag="VHV99_21550"
     CDS             complement(4792..5361)
                     /locus_tag="VHV99_21550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638016.1"
                     /translation="MKIRILTIACVTTAAALLAGCTAVYKNSDACEQLMRSKLAETSP
                     DTLKITHTGTGIDGSRVVVEGSIEHVVTASEVAAPAAASAAKLANAPRAASGVSAAST
                     VPAASSAADAAAASGSMAGAASGAAAASASVAASGAKATSIKAAKPKKTTTAAAAECT
                     FHGSVLGAFSWLAPATLIKPADKASEAAE"
     gene            5835..6458
                     /locus_tag="VHV99_21555"
     CDS             5835..6458
                     /locus_tag="VHV99_21555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium resistance transporter"
                     /protein_id="HEX3638017.1"
                     /translation="MFNLALLAVAAYVATNIDNLFVLLAFLGEAAGQRRHVIAGQFAG
                     SLTLIVGSILFAALLTRLPTGYVGLLGVLPIGVGLGKAWTRFRPGGADQEEEAQAAAR
                     KREPGAGAGSSWWTVACVAVANGSVNLAVYVPLYASHSHSEGMFISLVFVAMIGLWCA
                     GAVWLVDHPLLGAPIRRYGTALLPLILLAIGVSVIVQNDALRIVFGI"
     gene            complement(6598..6924)
                     /locus_tag="VHV99_21560"
     CDS             complement(6598..6924)
                     /locus_tag="VHV99_21560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638018.1"
                     /translation="MKRASTSRVAAVLLCAAACGVAAQAGAQGLSVQDDINAPQNSTR
                     VMPNAARGSEYPTHGNQQAGELNLNPTDPRAQLVNGLPNNTQSGGYGSPLAGVRTYVQ
                     PQMPAN"
     gene            7250..8128
                     /locus_tag="VHV99_21565"
     CDS             7250..8128
                     /locus_tag="VHV99_21565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX3638019.1"
                     /translation="MGAMNFLPIPQARSLRSTFAALSAALLLAACAAPTASTSFTAAD
                     SASLDAAISGPQRSDKNRARDVYRHPKDTLQFFDVRSSQTVLEIAPGGGWYTEILAPY
                     LHDHGKLYEAQYDSPDAASAAEEQAGRASFARKLAATPAVYGNVVVGTLRAGQFSGFP
                     ADGSVDQVLTFRNIHNWIKDGQIDANLRAFYTALKPGGVLGVEEHRAAPGTSLQQIID
                     TGYVTEAYVIEHARAAGFELLGESEVNSNPRDTKNYPHGVWSLPPTYEGHDVDRAKYA
                     AIGESDRMTLRFVKPR"
     gene            8492..10210
                     /gene="leuA"
                     /locus_tag="VHV99_21570"
     CDS             8492..10210
                     /gene="leuA"
                     /locus_tag="VHV99_21570"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEX3638020.1"
                     /translation="MLKNPATKYHSFKPINLTDRQWPSRVITRAPIWMSTDLRDGNQA
                     LFEPMNAQRKMRMFKTLVQIGFKEIEVAFPSASQTDFNFVRELIEGGHIPDDVTIEVL
                     TQARDDLIERTFESLRGAPRAIVHLYNATAPEFRRIVFGLEKSGVKELAQNAARTMKR
                     LVDATPETHFTLQYSPEVFSGTELEFAKEVCDAVFDIWQPTPEHKAIVNLPATVEMAT
                     PNVYADQIEWMHRNLARRDSLIVSVHPHNDRGTAVAAAELAVMAGADRVEGCLFGNGE
                     RTGNVDLVTLALNLYTQGVDPELDFSNINEVARTAEECTQLPVHPRHPYVGDLVFTAF
                     SGSHQDAIKKGFAVQKPDAVWEVPYMPIDPSDLGRTYDSVIRVNSQSGKGGIAYLLEQ
                     GYGVVLPRRLQVDFSSAVQRLTDSSGQEVTPAQIWELFQQEYVQNAAPIHYVGHSLSE
                     CDGREHIKLTVEIHGSRRVLNGEGNGPLDALMHAIGAPVRIQHYEERALTQGADARAV
                     AVAEMAGAEVAGGAFGVGIDANLVTASIRAVISGVNRAYARAGVEAQGRFFEVAMSDD
                     AEGVVV"
     gene            complement(10502..11290)
                     /locus_tag="VHV99_21575"
     CDS             complement(10502..11290)
                     /locus_tag="VHV99_21575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX3638021.1"
                     /translation="MIRLEKIDKYFGENRVLRSVDLQLASGNVTALVGPSGSGKSTLL
                     RCVNLLEIPESGSLELGDQRLEFSRDHKPSREAVLTIRRRTGMVFQNFQLFPHLTVRQ
                     NVMEGLITVLKWDKEKARVRADELLEKVGIAHKADAWPSTLSGGQQQRVAIARALAPS
                     PEVLLCDEPTSALDPGLAAEVVEVLKQLATEGMTMLMATHDLRLAATIARDVVFLNNG
                     VVIEAGPSRDIFMNPREPETARFVSTLTHSLPDAWTEQGGLEGE"
     gene            complement(11290..11967)
                     /locus_tag="VHV99_21580"
     CDS             complement(11290..11967)
                     /locus_tag="VHV99_21580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX3638022.1"
                     /translation="MPAWLHLMAQSLWPLLYAGLVFTVPLTLISFAIGLALAFIVALV
                     RLFGPRWAVAVVRFYVWLFRGSPLLVQLFVIFYGLPNVGIVLDPLTAAIIGFSLNVGA
                     YNSEVIRGVIESIPKGQWEAAYSMGMTRSQALRRAILPQAARVALPPLSNSFIALVKD
                     TSLAAVLTVPEVFQAAQRIASVTYEPLILYTEAALVYLVFSSVLSSAQVRLERKFGRH
                     ALFQAGN"
     gene            complement(11990..12772)
                     /locus_tag="VHV99_21585"
     CDS             complement(11990..12772)
                     /locus_tag="VHV99_21585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006052533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX3638023.1"
                     /translation="MKSIRSILLIVLLQAVTLSPAFAADELAQIKSAGVFKIGTEGTY
                     APFTYHDESGKLTGFDVEIGTAIAQRLGVKPQFVEGKWDGLIAGLDVNRYDAVINEVA
                     VTDARKAKYDFSDPYITSHAALIVASNNTTIKTFDDLKGKKSANTLTSNFGKIAAAHG
                     AEVIPVQGFNESIDLLTSGRVDATVNDSLSFLDFKKHKPDAKVKIATLDTSADSSDKS
                     AVLIRKGSPELQAAINKALADMRKDGTYAKISQKYFGKDVSQ"
BASE COUNT         2484 a         4094 c         3925 g         2573 t
ORIGIN      
        1 ccagtgacac ttgcttcgac ttcgagtcgc ttttgtagtc gattttggtc ggcgacgagg
       61 tgctgcagcc ggcgaccaga ccgcctgcca tcagcattac tgcaaaccgc ttggtaagac
      121 gaagatcagt catgttcagg gaatccttcc gctacgccac ggcaagtttc cgtggatcaa
      181 cccaacattt taccgatccc ggcgagacat gtgcaaaaac tggggttggc gcgcgaattg
      241 agcggcccgc ggccgaccga acgcagcagc gcgtggatcg gcgtggaggc ggctgaatcg
      301 ccacgatcca gcgagcctcc cggtgcgaaa agaggctccg ctccgctgca gtgatccgcg
      361 ctacaagccg ccaatttaac aatccacaac acttcccaat cgatcgtcgg atatgccgca
      421 cacgatcgtc agatcattga tttaaaaggc actttcgcgc ctggactaac cctattttcg
      481 agattacccg aagcgccgca caccgcacct gacaacgatc aggaaacctg agtgttatct
      541 tgaattagac gtctcaataa agattgaacg cgccaaccgc gagccagcta gccatgccaa
      601 atccttgttt ttcacccgcc ctttcacccg tcatcgccgc ctcggcaatc gtggctgccc
      661 ttctgtgcgt cagtgccgat gcacgtgccg aattgggcgg cacgatgccc agccatgcgg
      721 attcgacggc tgctgcgccc caaaccctgc tgaacggcgc cttacgaatg cgcacgctaa
      781 ccgatgctgg caataccacc atcaacgagt acgcgaccaa taccggccag atcattgcct
      841 acacgtggca gggcccgacc atgccggatc tgcgcgcgtt gctgggcaag tacgccgact
      901 cttaccggac gggcgccacc gcatcggcgc cggacggcaa tctgcacgcc tcgcgggtgg
      961 cccgcccgga cgtgatcgtg gaatccggcg gcccgatgcg cggctatgca ggacgcgcgt
     1021 ggctgccggc ggccttgccg gctggcgtct cggcagccga tttccgctaa cgccgcgcgc
     1081 acgccatcaa gaaaacgtct cgacgatccg ttgccgacat caacatgaag actccttctc
     1141 gtttccatgc cctgctcctc gtctgcgcgg cactcgtcac tggctgcggc ggtggcggcg
     1201 acaccgcttc ctctcaggca ggcgcagcca gcccggccag cagtccgggc gcggcgggct
     1261 cgtcaggggt tagcgcgcca tccgcgggtg gcctctccag cacggccacg tccgtgccgc
     1321 agtcgaccac gcccaacgtc cagccgatca cggtcggcgc cgtgccgggt ctgacgcgca
     1381 acatcctgac gacgagcgtc acggtctgcc agcccggcac cagcagctgc gcgacgatcg
     1441 acaacataca ggtcgacacc ggctcgcacg gcttgcggat cctcgcttcc gcgttgccgt
     1501 ccagcctcac gctcgcagcg gtgggttcag gcagcggcgt gacgggtgag tgctcagtat
     1561 tcggcagcgg ctatacgtgg ggctcggtgc gcagcgcgga cgtacggatg gccggccaac
     1621 tggccgcgtc gcttccgatc cagctgatcg ccgaccccac gctgcccact gtgccctctg
     1681 actgcgccag ctccggccca tcgatgctga ccggctcgaa accctatgcc aacggcattc
     1741 tcggcgtcgg cctgttcgcc gccgactgcg gcagcggttg cgtcaattca gctctgcccc
     1801 gctggtatta caactgcgac ggcagcggcg cctgcctcgc gagtacgcaa gcgctcgcgc
     1861 agcaggtcac caatccagtc agccgtttcg cgctcgacaa caacggcgtg gtgatcgacc
     1921 tgccggccat cgccgatgcg ggcgcggcca gcgtgtcagg ctcgatgatc ttcggcatcg
     1981 gaacccaggc gaacaacacg ctcgccggcg caagcgtcgt caaggcgaat caggtcacag
     2041 ggtgggtggt caccagcagc gccggccaga cttatgcgca gagctacatc gacagcggct
     2101 cgaatggctt gttcttcagc agtagccaat tcccgcaatg cggttactgg tattgcccga
     2161 gctcgacgca aacagcgaat gcctcgatca cgggcaccga cggcgtgtcg accacggtgt
     2221 cgtattcgat cggcaattcg cgtgcgctgt tcgcgtcgac gaataacgcg ttcaacaatc
     2281 tggctggggt tgtcagcaat ggcttcggct ggggcctgcc gttctttttc ggacggcggg
     2341 tctatacggc gatcgcgtcg cgcgctacct cggccggccc aggtccgtac tacgcgttct
     2401 gagtgcttga tgcccgagga tccaccttgc gaaatgtcta ctatcctgga tttctgcggg
     2461 ccgtctgcag acagcggcgc cgggcgggtt tgattgagca tctaagcaga ctctccctgt
     2521 cattagcgaa cccccacacc aaaacacaat aaatcccaca gaccgatcag acttacccgc
     2581 agggaaattt cctaaaccct catcccagac ttatttgata tggcctgact gcaataatca
     2641 tcgtttccgg ctagcgtcat ctcgatatcg ttagtcctgc atatctatca aagatcaaac
     2701 gatggaactc acgttttcgc tcgaccattt cgagcagctc gtttatacgc aacagcatgc
     2761 gcaggcctcg gtccagctca ttgccgcact cgcactcctc caacacacgc gcggcgaact
     2821 ggacgcaaac ttccaggcgt caggcatgac cgacctgcct ccccaggggc aacgccagcg
     2881 tttttgcacg cgcctgacga gcgccgtatc aacgttgttc gccgaccctg acttccggcc
     2941 gcccaccgag tgcttcaggt tgtttctcac gctgcatgaa tggatcggcg tgctgttctc
     3001 agccaccgcg tcaggcaatg cggaccatgt cgcccgctat ctgaatccgc gcggcccggc
     3061 ggatgcgcaa ttcctgccgg gagatagctt catcgagaag ctatgcgtgc tctattcgac
     3121 cgagtcagaa ctggaactcg acttcgccgc gctctgggcg tacgacaaaa ccatcgcggc
     3181 ctgtctcgcc ctcgttttgc tggcaccggt gttcaagggc tcgccgagcg cgcatctcaa
     3241 gcgcgaggcg ttgctggaat ggctgcccgg caggctgcag caaatcgacg acctcgacga
     3301 cctgccgtcg gccgtgctgc ataacgccta tatgttctgc agctatgccg acacgccgcg
     3361 gcggcatgcg gtcaagcagg acatcaatgt gctggtacgt cgcaagctgg cacaactcgg
     3421 cctgaccgac ctgcccgcgt caaaacgcac gccggtcaaa agcaaatcgc ggcgcggcaa
     3481 gaagcccgtg atggccgtcg tgctggagtg gttcagcggc gcacattcga tctaccgcac
     3541 gcactcgcgc acgctcgaag ccgcgcgcga acatttcgaa gtggtgggct tcggcttcgg
     3601 ttacgccgtc gacgacgtgg ggcggaagat cttcgatcgc ttcatcgaac tggagcagcc
     3661 ggaatacatc ggcgagtgtc tgaagacgat tcgcgacttt gccgaggccg aacagccgga
     3721 cgtgttgtac atgccgagcg tcggcatgtt cgtgctgacg gtgttcgtgt ccaacctgcg
     3781 cgtcgcgccg ctgcagatcg cggcgctcgg ccatcccgcc accacgcact ccaacaagat
     3841 cgactacatc agcgtcgaag aggactatgt cggcgacccg gcctgcttca gcgaacagct
     3901 gatgaagctg ccgaaagacg ggcaaccgta ccgcccgtca gtcgcactgc cggacatcgt
     3961 cgcgcggatt ccgccgccgc gcgaaacgct gcaggtggtc atcactgcca gcgcgatgaa
     4021 gctcaatccc ggctttcttg atgcatgccg cgcaatcggt cagcgggccg cgacgcctgt
     4081 tgagtttcac ttcatgagcg gtgtgccgtc cggtttgccg tttgaccgcc tgcgcgatgt
     4141 catcgcgcag gcgctgccag gcgccgtcgt tcacgacttt cacgactacg ccgcgtatct
     4201 ggcgcgcgtc aaccagtccg acttgttcct cagcccgttt ccgttcggca acaccaacgg
     4261 tattgtcgac gccttgacgc tcggcttgcc cggtgtctgc aagcgcggtc cggaagtgtt
     4321 cgagcgaatc gacggcgcgc agttcgagcg cgtgggcatg ccgtcgtgga gcactacgga
     4381 cagcgtcgag gcttatatca ccgcagccgt gcggatgatc gaccagcacg acgagcgcac
     4441 cgcgctgcgc cagcgcctga tcgatacgca ggcggtagag cgctgctttg aagggcggcc
     4501 ggaggcattc ggcgaaagcg tgttgaaact gctgcgcaaa aacaaaaacc gccacgtgaa
     4561 cggggcggct tgaggtacgt cttgaggcac ggcttgcgtc ggccggatca tgccgtggcc
     4621 gttgagcacg gcacgaagta ctcagcggaa tgctgaccgg cggcaacagg cgcgacgggc
     4681 attcagactg ctgaccaacc ccacgttttc cagagcgtgc ggttttgttt ttcaggcggc
     4741 gcggatcgct gtgtggagtt gagtccgcgg gcaagcaggg tcaggcaatt gctattcagc
     4801 agcttcgctg gctttatcgg ccggcttgat gagcgttgcc ggcgccaacc agctgaatgc
     4861 gcccagcacc gaaccgtgaa acgtgcactc ggcggcggcg gccgtggtgg tcttcttcgg
     4921 cttggcggct ttgatcgagg tagctttggc ccctgacgcg gcgacgctcg ccgaagcagc
     4981 cgcagcaccc gaagccgctc cagccatcga gccggaagct gccgcggcgt ccgccgctga
     5041 agaagccgcc ggcaccgtgg atgccgcgct cacgcccgaa gccgcccgag gcgcattcgc
     5101 cagtttcgcg gcggaagctg ccgcgggtgc cgcaacttca gaagccgtca ccacatgctc
     5161 gatcgatcct tcgacgacta cccgggagcc gtcgataccg gtgcccgtat gcgtaatctt
     5221 cagcgtatcc ggcgacgttt cagccagctt gcttcgcatc aactgctcgc atgcatccga
     5281 gttcttatag acggccgtgc agcccgctag cagcgcggca gcggtcgtga cacaggcgat
     5341 agtgagaatc cgaatcttca ttgatgttcc gtttttcaag ccgtcggcaa ttgtagctta
     5401 cgggctgatg ggtcgacagc gaggcccacg gaaggggcgc aggaacggga caggccagtg
     5461 acaggcgctg acgcagaacg ggacgggaag aggcgggact gggctgggct ggacggggct
     5521 ggactggact ggactgggct gggctgggct ggactgggct gggctggacg agccgagcca
     5581 gcacgggacg ggacggggcc gcaccgcacc gcaccgcacc gcaccgcacc gcaccgcacc
     5641 gcacagcacc gcaaaatatt aagccaccag gcgcacacat cgcccaacag acagagacac
     5701 ggcttgaccg cgacgctctg catcggcatc gtgtcgatcg gccgccatgc ctcaggcact
     5761 gccgcctctt cggctagacc cgcgttgctg ggtatagacc cgttttccca ctgtgcattg
     5821 accgcctttc ccgcatgttc aaccttgccc tgctcgctgt cgcggcttac gtcgcgacca
     5881 atatcgacaa ccttttcgtt ctgctcgcat tcctcgggga agcggccggc caacggcgtc
     5941 acgtgatcgc cgggcagttt gccggttcgt tgaccttgat cgtgggatcg attctgttcg
     6001 ccgcgttgtt gacacgctta cccaccggct atgtcggcct gctcggcgtc ttgccgatcg
     6061 gcgtgggtct gggcaaagcg tggacgcgct ttcgccctgg cggcgcggat caggaggaag
     6121 aggcgcaggc cgcggcgcgc aagcgtgaac cgggagccgg cgcaggctcc tcatggtgga
     6181 cagtcgcatg cgtggcggtc gccaatggtt cggtcaatct tgccgtctac gtgcccttgt
     6241 acgcgagtca ctcgcacagc gagggcatgt tcatctcgct ggtgttcgtc gccatgatcg
     6301 gcctctggtg cgctggcgcg gtgtggctgg tggaccatcc gttgctgggc gcgccgatcc
     6361 ggcgctacgg aaccgcgttg ctgccgctga tcctgttggc gatcggcgtc tcggtgatcg
     6421 tgcagaacga tgcgctgcgg atcgtgttcg gcatctgagt gagaccgccg ggaacaatca
     6481 caatccgatg atcgtgctat cggaccacac gccggaacgg aaaatcgcgg tccacgccag
     6541 gatgcgctgc tgtcagcgcg cgatgcagca ggaccggtct tcgcccgcgc gaaacgctca
     6601 gtttgcgggc atttgcggct gcacgtaggt gcgcacgccg gcaagcggcg aaccgtagcc
     6661 acccgactgc gtgttgttcg gcaaaccgtt gaccagttgc gcgcgcggat cggtcggatt
     6721 cagattcagt tcgcccgcct gctggttgcc gtgcgtcgga tattcgctgc cgcgcgccgc
     6781 attcggcatc acacgcgtac tgttttgcgg cgcgttgatg tcgtcctgca cgctcaggcc
     6841 ctgcgcacca gcctgtgcgg cgacaccgca cgcagccgcg cacagcaata ccgccgctac
     6901 cctcgacgtc gatgctcgtt tcatacctgc ctccagcaaa gtgaaatgaa aggcgaccat
     6961 gtcagcgagt tcaataccgg cgagccagcg cgctgacaga cgctgaacac attatttata
     7021 cgcgacagtg cgtctttcgg attcggctaa atctgcaccg ccggccttaa atacgcgccg
     7081 ccccttcctt cgaattaggc gaaatcctcg ccgtccgcta caaatcggca gagcgggcgc
     7141 aaaacgtggc ctggccgaag catccaggga cacggggaca tagcaaatac cgctggcgca
     7201 taaggttatg tcgatcggcg atttctttcc aggcggatcg cgcccgataa tgggcgccat
     7261 gaacttcctc cccattcccc aagcccgctc gttgcggtcc acgttcgcgg ccctgagcgc
     7321 cgcgctgctg ctggccgctt gcgccgcccc aacggcgtcg acctctttta cagccgctga
     7381 ttcagcctcg ctcgatgccg caatttccgg accgcagcgc agcgacaaga accgcgcgcg
     7441 cgatgtgtac cggcatccaa aggacacgtt gcaattcttc gacgtgagat cgtcgcagac
     7501 cgtgctggag atcgcgcccg gcggtggctg gtacacggag atactcgcac cctatttgca
     7561 cgaccacggc aaactgtacg aggcgcaata cgacagcccg gacgcggcgt cggccgcaga
     7621 ggagcaggct gggcgggcgt cgttcgcgcg caagctcgcg gccacaccgg ctgtgtacgg
     7681 caatgtggtg gtcggcacgc tgcgagcggg ccaattcagc ggttttccgg ccgacggcag
     7741 cgtcgaccag gtgctgacct tccgcaatat ccacaactgg atcaaggacg gccagatcga
     7801 cgcgaacctg cgcgccttct atacggcgct taaaccgggc ggcgtcctcg gtgtcgagga
     7861 acatcgcgcg gcgccgggca cttccctgca acagatcatc gacaccggtt atgtgaccga
     7921 ggcctatgtc atcgaacacg cgcgcgccgc aggcttcgag ttgctcggcg agagcgaggt
     7981 gaacagcaat ccgcgcgaca cgaaaaacta tccgcacggc gtgtggtcac tgccgcccac
     8041 gtatgaaggc catgacgtcg atcgggcgaa atacgcggcg attggcgagt cggatcgcat
     8101 gaccttgcgt ttcgtgaaac ccaggtgacg tacacgacgg gccggggttt cagtacgtcc
     8161 ccgctgggaa aacccctccg aacggcacgt tttctcgacg ggaaaggccg tcgcagaaag
     8221 cccggcgcgg attgcttcgt ctcaccgcgc ttgacgccgt cctaataata tggtcctatt
     8281 gcgcactatg taccgcgccc agatcactct atcgctacta ctagcccgct ctaccgggct
     8341 aggtctgctg ctgcgcgctt ccatctgata cacccgaagc accgctgaat tccccagtaa
     8401 ctgacccagc cccggtttcc gaccggcggc catgcttttg ttttcgtcgt ccggtcgaca
     8461 cccaggttga tcgatcccaa ggatgatgaa aatgttgaaa aaccctgcta ccaaatatca
     8521 ctcgttcaaa cccatcaatt tgacggaccg ccagtggccg tcgcgcgtca tcacgcgcgc
     8581 gccgatctgg atgagtaccg acttgcgtga cggcaaccag gcgctgttcg agccgatgaa
     8641 cgcgcaacgc aagatgcgca tgttcaagac gctggtgcag atcggtttca aggaaatcga
     8701 agtcgcattt ccgtctgcct cgcagaccga cttcaatttc gtgcgcgagc tgatcgaagg
     8761 cgggcatatc cccgacgacg tgacgatcga agtgttgacg caagctcgcg acgacctgat
     8821 cgaacgcacc tttgaatcct tgcgtggcgc gccgcgcgcc atcgttcacc tgtacaacgc
     8881 gactgcgccg gaattccgcc gcatcgtgtt cggcctcgaa aagagcggcg tcaaagaact
     8941 cgcgcaaaac gccgcgcgta cgatgaagcg tctcgtcgac gccacgccgg aaacacactt
     9001 cacgctgcaa tacagcccgg aagtgttcag cggcaccgag ctcgaattcg ccaaggaagt
     9061 gtgcgacgcc gtgttcgaca tctggcaacc gacgcccgaa cacaaagcca tcgtcaactt
     9121 gcccgcgacc gtcgaaatgg ctacgccgaa tgtgtacgcg gaccagatcg aatggatgca
     9181 ccgcaatctc gcacgtcgcg attcgttgat cgtttcggtg catccgcata acgatcgggg
     9241 cacggctgtg gctgccgcag aactggcggt gatggcggga gcggatcgtg tcgaagggtg
     9301 tttgttcggc aatggcgaac gtaccggcaa tgtcgatctc gtgacgcttg cgttgaatct
     9361 gtacacgcag ggtgtcgatc ctgaactcga cttttcgaat atcaatgaag tggcgcggac
     9421 cgctgaggaa tgcacgcagt tgccggtgca tccgcgtcat ccgtatgtcg gcgatctggt
     9481 gttcaccgct ttctccggct cgcatcagga tgccatcaag aaggggtttg ctgtgcagaa
     9541 accggatgcc gtctgggaag tgccttatat gccgatcgat ccgagcgatc tcgggcggac
     9601 ttatgattcg gtcattcgtg tgaatagtca gtcaggcaaa ggcgggatcg cttatttgct
     9661 cgaacagggt tatggggtgg tgctgccgcg gcgcttgcag gttgatttta gttctgctgt
     9721 gcagcggttg actgattcta gtgggcagga ggtcacgcct gcgcagatct gggaattgtt
     9781 ccagcaggag tatgtgcaga atgctgcgcc gattcattat gttgggcata gcctgtctga
     9841 atgcgatggg cgtgagcata tcaagctcac tgtcgagatt catggctcgc ggcgcgtttt
     9901 gaatggggaa gggaatgggc cgctcgatgc tttgatgcat gctatcggcg caccggtgcg
     9961 gattcagcac tatgaggagc gggctttgac tcagggtgcc gatgctcgcg ctgtggctgt
    10021 tgctgagatg gctggggctg aggtcgctgg gggcgcgttt ggggttggta ttgatgctaa
    10081 tctggttacg gcttcgattc gggctgttat tagtggggtt aatcgcgcgt atgcgcgggc
    10141 tggggttgag gctcaagggc ggttttttga agtcgctatg agtgatgacg cggagggtgt
    10201 ggttgtgtga cggcttttgg tttttttgtt gttggccttt ccttgttttg ttagtggtct
    10261 attagcgtcg cccctgtgcg gggcggcacc tacttttctt tgcctgccgc aaagaaaagt
    10321 aggcaaaaga aagcggctca ccccgccagc tcataagcgg ggctcccgca cagccacggt
    10381 agtggtgcat ctggaatctg tgctctcgcc cattcggcgc gcgtgacaag gcagtcttac
    10441 ttcccgcctc gcactgcgtg ctcgccggaa gggtctgctc gcaaaccagt gggtacatcc
    10501 cctactctcc ctccaggccg ccctgctccg tccatgcgtc cggcaagctg tgcgtcagcg
    10561 tcgagacgaa gcgcgctgtc tccggttcgc gcgggttcat gaaaatgtcc cttgatgggc
    10621 ccgcctcgat caccacgccg ttgttcagaa acaccacgtc tcgcgcaatc gttgcggcca
    10681 ggcgcaaatc atgtgtcgcc atcaacatcg tcatgccttc ggtggcaagc tgcttcagga
    10741 cttccactac ctccgcagcc agtcctggat cgagagccga ggtaggttcg tcgcacaaca
    10801 atacttccgg tgatggggcc agcgccctcg caatcgccac gcgttgctgc tggccgcccg
    10861 acagcgtcga gggccatgcg tccgctttgt gcgcaatgcc gactttttca agtagttcgt
    10921 ccgcccggac gcgcgccttt tccttgtccc acttcagtac ggttatcaag ccttccatca
    10981 cgttctggcg taccgtcaga tgcggaaata actgaaaatt ctggaacacc atgccggtgc
    11041 ggcggcggat cgtcagcacc gcctcgcgcg atggcttgtg atcgcggctg aattcaaggc
    11101 gctggtcgcc aagttcaagt gagccggact cagggatttc cagcagattc acgcaccgca
    11161 aaagcgtact cttgccgctg ccggacggac caaccagtgc cgtgacatta cccgacgcaa
    11221 gctgcaggtc cactgacctc agcacgcgat tctcgccgaa gtacttgtcg attttttcca
    11281 gtcggatcat cagttgcccg cctggaacag tgcgtggcgg ccgaacttgc gctcaagccg
    11341 cacttgcgcc gacgacaaca ccgaactgaa cacgagataa acgagtgccg cctcggtata
    11401 gagaatcaac ggttcatatg tcaccgacgc aatccgctgt gccgcctgaa atacctcagg
    11461 caccgtcagt accgcggcaa gcgaagtgtc cttcaccaat gcaatgaacg aattcgacag
    11521 cggcggcagc gcgacgcgtg ccgcctgcgg caagatcgcg cggcgcagtg cctgtgaacg
    11581 cgtcattccc atcgaatacg ccgcttccca ctgccctttc ggtatcgact cgatcacgcc
    11641 gcgtatcact tccgagttat acgcgccgac attcagcgaa aaaccgataa tcgccgctgt
    11701 cagcggatcg aggacgatgc cgacattcgg caagccgtag aaaatcacaa acagttgcac
    11761 gagtagcggc gagccgcgaa acagccatac gtaaaaacgc acgaccgcca ccgcccatct
    11821 cggcccgaac agccgcacca gcgcaacgat gaacgccagc gcaagcccga tcgcgaacga
    11881 tatcaaggtg agcggcacgg tgaatacgag ccccgcatac aacaggggcc acagcgactg
    11941 cgccatcaaa tgcaaccatg ccggcatgat tcagactcgc aggtgacgct tattgggaaa
    12001 cgtctttacc gaagtacttc tgcgagatct tcgcgtacgt gccgtctttc cgcatgtcgg
    12061 cgagcgcctt attgattgct gcctgcaact ccgggctgcc cttgcggatc agcaccgcgg
    12121 atttgtcgct gctgtcggcc gacgtatcga gcgtggcgat cttgaccttc gcatccggct
    12181 tgtgcttctt gaagtcgagg aacgacagcg aatcgttcac ggtcgcgtcg acgcgccccg
    12241 aagtcagcag gtcgatcgat tcgttgaagc cctgcacagg aatcacttcc gcgccgtgcg
    12301 cggccgcgat cttgccgaag ttgctggtca gcgtattggc cgacttcttg cccttcaggt
    12361 catcaaaggt cttgatcgtg gtgttgttcg aggccacgat cagcgccgcg tgcgaggtga
    12421 tgtacgggtc cgagaaatcg tatttcgctt tgcgtgcgtc ggtcaccgcg acttcattga
    12481 tcaccgcgtc gtagcggttc acatccagac cggcgatcag accgtcccat ttgccctcga
    12541 cgaattgcgg cttgacgccc aggcgctgcg cgatcgccgt gccgatctcg acgtcgaagc
    12601 cggtcagctt gccggattca tcgtggtacg tgaacggtgc ataggtgcct tcggtgccga
    12661 tcttgaacac acccgcggac ttgatctgcg cgagctcgtc agcagcgaat gcggggctca
    12721 gggtcaccgc ttgcagcagc acgatcagta gaatggaacg aatcgacttc atcaggtgcc
    12781 tccgtaaagt ttgctatgtt agttgcatgg ttttctatgc ggcattccgc cggcgcaaag
    12841 gctcggcaga gtgggcggac tgtagcaaac ggtctgcgtt tttacaaagg atcaggtttc
    12901 cgatcaatat gcgcatgcgt gctaagcgga cagccaaagc atcttacaca acgttgaatc
    12961 tgactttaca gcagatatga cagacgcggc atgcatcgca ccatggatcg cgtgccgggc
    13021 gccatcgggg aaaacgcggg agggtggcgg gagagtggcg ccacggcgaa cgcgcg
//
LOCUS       DATASH010000803         2327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_134659,
            whole genome shotgun sequence.
ACCESSION   DATASH010000803 DATASH010000000
VERSION     DATASH010000803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134659"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..964)
                     /gene="prmA"
                     /locus_tag="VHV99_21590"
     CDS             complement(62..964)
                     /gene="prmA"
                     /locus_tag="VHV99_21590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX3638024.1"
                     /translation="MSYRELVVELVREHADAFCDALLELGALSVSVEDADADTPDEQP
                     LFGEPGLTPDRSAWQRSRVIALLALDHEPAVLLTAAANEVGLDAAPSFTVRDVEDQDW
                     VRLTQSQFDPIPVGERIWVVPSWNDAPDPEALVLELDPGLAFGTGSHPTTRLCMEWLE
                     QSVEPGQSMLDYGCGSGILAILARKCGANPVYGIDIDPQAIESARHNSARNRAEVTYG
                     LPDECPSGAFDIVVANILSNPLKLMASMLSSKVKPGGRIALSGILARQADEVAQVYAR
                     WIDIGVWREHEGWVCLAGTRRESH"
     gene            complement(968..>2327)
                     /gene="accC"
                     /locus_tag="VHV99_21595"
     CDS             complement(968..>2327)
                     /gene="accC"
                     /locus_tag="VHV99_21595"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017777083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX3638025.1"
                     /translation="KILIANRGEIALRIQRACRELGVKTVVVYSEADKEAKYVKLADE
                     AVCIGPAPSNLSYLNMPALISAAEVTDAEAIHPGYGFLSENADFAERVEQSGFTFIGP
                     RPETIRMMGDKVTAKQTMIKTGVPCVPGSEGALPDDPKEIVKIARQVGYPVIIKAAGG
                     GGGRGMRVVHTEAALVNAVNMTREEAGRAFGNPQVYMEKFLENPRHIEIQVLSDSFRN
                     AIWLGERDCSMQRRHQKVIEEAPAPGIARRLIDRIGDRCADACKKMGYLGAGTFEFLY
                     ENGEFYFIEMNTRVQVEHPVTELITGVDIVQEQIRIAAGEKLAFRQRDITFKGHAIEC
                     RINAEDPFKFIPSPGRLTSWHMPGGPGIRVDSHAYNGYFVPPNYDSMIGKLIAYGATR
                     EQAIMRMRIALSEMVVEGIQTNIPLHRELMLDAKFVEGGTSIHYLENRLAAKLQAAPE
                     EA"
BASE COUNT          404 a          746 c          720 g          457 t
ORIGIN      
        1 tcgccaggag catattgagc cgataagccg gtaggctgtt ggttagacaa tacgccttat
       61 tctaatggct ttcgcgacgc gttcccgcta gacacaccca accttcatgt tcgcgccaga
      121 cgccgatatc gatccagcgc gcgtacacct gcgcgacttc gtccgcctgg cgcgcgagaa
      181 tgccggatag cgcgatgcgg ccgcctggct ttaccttcga cgacagcatc gacgccatca
      241 gcttgagggg gttggagagg atattcgcga ccacgatatc gaatgcgccg ctcggacatt
      301 cgtcgggcaa accgtacgtg acttcggcgc ggttgcgcgc gctgttgtgc cgcgccgact
      361 cgatcgcttg cgggtcgatg tcgatgccat acaccgggtt ggcgccgcat tttctggcaa
      421 gaatggcgag gatgccggag ccgcaaccgt aatcgagcat ggactgcccg ggctcgaccg
      481 attgctccag ccattccatg cacagccgcg tggtgggatg gctgccggtg ccgaacgcga
      541 ggcccgggtc cagctccagc accagtgctt cggggtccgg tgcatcgttc cacgaaggca
      601 ctacccagat gcgctcgcca accggaatcg gatcgaattg cgattgcgtg agccgcaccc
      661 agtcctggtc ttcgacgtcg cgcaccgtaa acgacggtgc ggcatcgagc ccgacctcgt
      721 tcgccgcggc ggtgagcagc accgcgggtt cgtggtccag cgcgagcaac gcgatcacgc
      781 gcgaacgctg ccacgcggag cgatccggcg taagaccggg ctcgccgaac agcggctgtt
      841 cgtcgggcgt gtcggcgtcc gcgtcttcga ccgataccga cagcgcgccc aactcgagca
      901 atgcatcgca gaatgcatcc gcgtgctcgc gcaccagttc gacgaccagt tcccgatagc
      961 tcatgactta cgcttcttcc ggcgcggcct gcagcttcgc ggccaaccga ttttcgaggt
     1021 agtggatgct cgtgccgcct tcgacgaact tggcgtcgag catcaactcg cggtgcagcg
     1081 ggatgttggt ctgaataccc tcgaccacca tttccgacaa cgcgatgcgc atccgcatga
     1141 tcgcctgttc gcgcgtggcg ccgtaagcga tcagcttgcc gatcatcgaa tcatagttcg
     1201 gcggcacgaa atagccgttg tacgcgtgcg aatcgacgcg gatgccgggg ccgcccggca
     1261 tatgccacga ggtcaaacgg cccggcgacg gaatgaactt gaacggatct tcggcgttga
     1321 tccggcattc gatcgcatga cccttgaacg tgatgtcgcg ctgacggaat gcgagctttt
     1381 caccggccgc gatgcggatc tgttcctgca cgatgtcgac gcccgtgatc agctcggtca
     1441 ccgggtgctc gacctgtacg cgcgtgttca tttcgatgaa gtagaactcg ccgttttcat
     1501 acaggaattc gaacgtgcct gcgcccagat agcccatctt cttgcaggcg tccgcgcaac
     1561 gatcgccgat acggtcgatc agacggcgcg cgatgcccgg cgccggcgct tcttcgatca
     1621 ccttctggtg acggcgctgc atcgagcagt cgcgctcgcc gagccagatc gcgttcctga
     1681 acgaatcgga cagcacctgg atttcgatgt gccgcgggtt ctccaggaat ttctccatgt
     1741 agacctgcgg gttgccgaac gcgcggccgg cttcttcgcg ggtcatgttg accgcattga
     1801 ccagcgccgc ttccgtgtgg accacgcgca taccgcggcc accgccgcca ccggcggcct
     1861 tgatgatgac cggatagccg acctggcggg cgatcttcac gatctctttc ggatcgtccg
     1921 gcaacgcgcc ttccgaaccc ggcacgcacg gcacgccggt cttgatcatg gtctgcttgg
     1981 ccgtgacctt gtcgcccatc atccggatcg tttccgggcg cgggccgatg aaggtgaagc
     2041 ccgactgctc gacgcgctcg gcgaagtcgg cgttctccga aaggaagccg tagccggggt
     2101 ggatcgcttc ggcgtcggtc acttcagccg cgctgatcag cgcgggcatg ttcagatagc
     2161 tcagattcga cggtgccggg ccgatgcaga ccgcctcgtc ggcgagcttc acgtacttgg
     2221 cttccttgtc ggcttccgaa tagacgacga ccgtcttgac gccgagttcg cggcaggcgc
     2281 gctggatacg aagcgcgatc tcgccacgat tggcaatgag gattttt
//
LOCUS       DATASH010000804         7715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5675689,
            whole genome shotgun sequence.
ACCESSION   DATASH010000804 DATASH010000000
VERSION     DATASH010000804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7715
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5675689"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..2183)
                     /locus_tag="VHV99_21600"
     CDS             complement(78..2183)
                     /locus_tag="VHV99_21600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018431635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEX3638026.1"
                     /translation="MLNRKSTSSSPRWSSRLYGSLYALVTAIYRGRPVWMVSFSTSEA
                     NLSEGLRAACASTAMLVLGNLLHDPLFAWAAIGAFWTCLADAAGSNRTRFASMMGFAL
                     LSTVCGGVTAFASGEGAVLAALAILVFTTLGAFGRIWGAATSQVTILAATACVVMVDR
                     PMHDFRGGMAFLGIYLVGCLFAVVLSLSIWRIHPFGPSRAALRAVYVRLADIAFDSAR
                     LLEQRAAPGQWATHAAKFRADARASLERSRKVLANVPGSRTAGRETYDNLLALLSESE
                     ALFAYLIAVTGACEKAPEDARRVKRTARLLTGMGETLRRIGAAANEAQWNRLAGLQLR
                     LRSLARRLESALMEPVTLKADFELVDFTPTHAQPMGWSDSAAGLLTRTWSTLKANLSL
                     QSVGLRHAARVGVTTTAGFLVIRALGLPFGYWATMATLLILQPSIAATWPRSIERAAG
                     SIVGGVLAAAIGYAIHSPIGISLAVFPLVVATMALRPVSYSLFVLFLTPTFVLVADFA
                     TPGANEFVYALTRLGNNVLGCVLALLATFYLWPTREKVDYRAYLGEAVRANLAYLRAA
                     LESPRRSERDMERLRRAAGLGSNNAEEAIGRIRLEKLEDSMVDTVTLTVLSVLRKMAG
                     TATQLRLSTNRRDMHDELGRWVAAASADIEAALERNLRPIRRDLPARDGLTSLEADAV
                     GEIALMHRLLTEQMREARG"
     gene            complement(2477..3004)
                     /gene="ppa"
                     /locus_tag="VHV99_21605"
     CDS             complement(2477..3004)
                     /gene="ppa"
                     /locus_tag="VHV99_21605"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007587974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEX3638027.1"
                     /translation="MSFNHVPAGKDLPQDFNVIIEIPAQSDPVKYEADKETGLLHVDR
                     FISTGMRYPANYGYIPQTLSGDGDPVDVLVITPFPLLAGSVVRARALGMLQMTDESGV
                     DAKLVAVAHDKVCPMTPDLKSIDDVPAYLKDQIKHFFEQYKALEKGKWVKVDGWAGIE
                     AAHKEITEGVANYKK"
     gene            3280..4719
                     /locus_tag="VHV99_21610"
     CDS             3280..4719
                     /locus_tag="VHV99_21610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011352864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX3638028.1"
                     /translation="MEEARHFIGGEWSAASGGETIAVIDPSDGQPFTELARGTAADID
                     AAVHAARRAFEGAWGAASAAERGRVLYRLSMLVAARQEELAQLEARDTGKPLKQARAD
                     SAALARYFEFYAGAADKLHGETLPYQAGYTVLTIREPHGVTGHIVPWNYPMQIFGRSV
                     GAALATGNACVVKPAEDACLSVLRVAELAAEAGLPAGALNIVTGFGHEAGAALARHPG
                     IDHISFTGSPETGKLVTQMAAENHVPVTLELGGKSPQIVFADADLDAALPVLVSAIVQ
                     NAGQTCSAGSRVLIDRAIYEPLLDRLSGAFHALRVGPSHADLDCGPLINAKQQQRVWD
                     FLSDAQHDGIPLAAHGEVIPEAPESGFYQAPTLLRDVPASHRLARDEVFGPVLAAMPF
                     TDEDEALALANGTQYGLVAGIWTRDGARQMRLARRLRSGQVFINNYGAGGGVELPFGG
                     VKHSGHGREKGFEALYGFTVLKTIAIRHG"
     gene            4822..5580
                     /locus_tag="VHV99_21615"
     CDS             4822..5580
                     /locus_tag="VHV99_21615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004543860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3638029.1"
                     /translation="MRLTGKTAIVTGGGSGFGEGIAKTYAREGANVVVNDLNGAAAER
                     VASEIALAGGKAIAVAGDVTQREDWRTLREAALEDFGSVQIVVNNAGTTHRNKPVLDV
                     TEAEFDRVYAVNVKSIYWSVQEFVPYFRAQGGGTFINIASTAGVRPRPGLVWYNGSKG
                     AVIIASKSLAVELGPDRIRVNCVNPVIGETALLSEFMGVEDTPENRKRFLAGIPLGRF
                     STPQDIANAALYLASDEAEFITGVCLEVDGGRCV"
     gene            5803..7122
                     /locus_tag="VHV99_21620"
     CDS             5803..7122
                     /locus_tag="VHV99_21620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3638030.1"
                     /translation="MASPANPLHHPGAGAPPSTFEEATYRKVTWRLAPLLMLCYVVAY
                     LDRVNVGFAKLQMTTDLGLSDAVYGFGAGIFFVGYFIFEIPSNVILHKVGARVWIARI
                     MVSWGVISMLTMFVTTPTMFYVMRFLLGLAEAGFFPGIILYLTYWYPSHRRGRMTTWF
                     MTAIALSGVIGGPVSGYILKSFNGMNGWHGWQWLFLLEGIPSVIVGVMVFLMLDDRIS
                     RAKWLTEEEKQLLERQVSAEEATKHDMPIRQVLTSGRVLMLSLTYFSFVMGLYGVSFW
                     LPTIIKATGVTDSFMIGLLSAVPFAAAVVAMVFVSRSADRTRERRWHIALPAFAGAIG
                     LVLSVVWAHNTVLAMASLTLATMGILTTLPLFWSLPTAILAGTGAAAGIAMINSIGNL
                     AGFLSPYAVGWLKQATAANDSGMYMLAAFMILGGLLAISVPAKMVNK"
     gene            complement(7417..>7715)
                     /locus_tag="VHV99_21625"
     CDS             complement(7417..>7715)
                     /locus_tag="VHV99_21625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004193604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3638031.1"
                     /translation="RYPDTTGGDALPLIQKAEGWQKDRPEDADLLFALGRLCLHQQLW
                     GKAQSFLERALKLADNETLKIRSHRALARLHEQLGDADKASQHYRESALAMNIV"
BASE COUNT         1418 a         2492 c         2431 g         1374 t
ORIGIN      
        1 gaacgagtcg agaatacaca tctgagaatc agaggggtgc gtcaagggct atcgcaagca
       61 atcctcatcc gcctcgccta ccccctcgcc tcccgcatct gctcggtcag cagccgatgc
      121 atcagcgcga tttcccccac cgcatctgct tccagcgagg tcagcccatc tcgcgcaggc
      181 agatcgcgcc gtatcggccg caagttccgc tccaaggctg cctcgatgtc ggcgctcgcc
      241 gcagcaaccc acctgccgag ttcgtcgtgc atgtcgcgcc ggttcgtact cagacgcagt
      301 tgcgtcgccg ttccggccat ttttcgcaac acgctcagca ccgtcagcgt caccgtgtcg
      361 accatcgagt cctcgagctt ttcgagtcga atgcggccga tggcctcctc ggcattattg
      421 ctgcccaagc cagcggctcg gcgcaaacgt tccatgtccc tctcgcttcg acgcggcgac
      481 tccagtgccg ccctcagata cgcgagattg gcgcgcaccg cctcgcccag ataggcgcga
      541 tagtcgactt tttcgcgcgt cggccacaga tagaacgtcg ccagcagcgc caacacgcag
      601 ccgagtacgt tgttgccgag ccgcgtgagc gcgtagacaa attcgttggc gcccggcgtc
      661 gcgaagtcag ccacgagcac gaaggtcggc gtcaggaaca gaacgaacag gctgtagctg
      721 accggacgca gcgccatcgt ggccaccacc agcggaaaga ccgccaatga aatgccgatg
      781 ggcgaatgaa tcgcatagcc gatcgcggcc gccagcacgc ccccgacgat gctaccggca
      841 gcgcgttcga tgctccgtgg ccaggtcgcg gcaatggacg gttgcaggat cagcaacgtc
      901 gccatggttg cccagtagcc gaagggaagc ccgagcgccc gtatgaccag aaagcccgcc
      961 gtcgtcgtga cgcccacgcg ggcggcgtgg cgcagtccga ccgactgaag cgagaggttt
     1021 gccttgaggg tcgaccatgt ccgcgtcaac agcccggctg cgctatccga ccatcccatc
     1081 ggttgggcat gggtcggcgt gaaatcgacc agttcgaagt cggccttgag cgtcacgggt
     1141 tccatcaacg cggattcgag tcgccgcgcg aggcttcgca agcgaagctg caggccggcg
     1201 agacggttcc attgcgcttc gttggcggcc gcgccgatcc ttcgcaacgt ctcacccatt
     1261 ccggtcagca gccgcgccgt gcgttttacg cgacgggcgt cttccggggc tttttcacag
     1321 gcgccggtga ccgcgatcag atacgcaaag agcgcttcac tttcgctcag cagggcgagg
     1381 aggttgtcgt aggtttcgcg gccggccgtt ctcgaaccag gcacgttcgc cagcaccttg
     1441 cgcgagcgct ccagcgaagc gcgcgcatcg gcgcgaaatt tcgcggcatg cgtggcccac
     1501 tgtccggggg ctgcgcgctg ctcgagcagg cgggcgctgt cgaatgcgat gtcagccagc
     1561 cgcacataca cggcacgcag cgcagcgcgg ctcgggccga acggatgaat acgccagatg
     1621 ctcaggctca gcaccacggc aaacaggcat ccaacgagat agatgccgag gaacgccatg
     1681 ccaccacgaa agtcgtgcat gggccggtcg accatcacga cgcaggcggt cgcggcgaga
     1741 atcgtcacct gcgatgtcgc ggcgccccaa atcctgccga acgcgccgag cgtcgtgaat
     1801 accagaatgg ccagcgccgc gagcaccgcc ccctcgcctg acgcgaaggc ggtcacgccg
     1861 ccgcacacgg tcgacaggag cgcgaagccc atcatcgagg cgaagcgcgt gcgattcgag
     1921 ccggcggcgt cggcgagaca ggtccagaaa gcgccaatcg cggcccacgc gaagagcgga
     1981 tcatgcagga gattgccaag caccagcatg gcggtcgacg cacaagcggc tctcagccct
     2041 tccgacagat tggcctcgct cgtcgaaaac gacaccatcc atacgggccg gccgcggtag
     2101 atcgcggtga cgagcgcata caggctcccg tacaagcggc tcgaccagcg cggcgacgat
     2161 gaggtggact tgcgattcag catccggcgg tagctcccaa aaacgcgtgg tggcgctgcg
     2221 tcagcacagt aatacgccga cggcaggaaa cctgctgcgg ccgccgaatg ataagggtta
     2281 acgctgacat ataaaaatgc cttcggttca tccagtcgat gcgatgtgtc tatgcgctgg
     2341 agccgccggc gagatgggcg cgcggccggc gctacgcgtt aggcgcgcag tggaatcgct
     2401 gaacacactg cccatgaaaa aaccgcgcgg cccttgcagg ctcgcgcggc tttttcatca
     2461 accggtgtaa cgcagcttac ttcttgtagt tcgccacgcc ttcggtgatt tccttgtgag
     2521 cggcttcgat gcccgcccaa ccgtcgacct tcacccactt gcccttctcc agcgccttgt
     2581 attgctcgaa gaagtgcttg atctggtctt tcaggtatgc cgggacgtcg tcgatcgact
     2641 tgaggtcggg ggtcatcggg cagaccttgt cgtgcgcgac ggcgacgagc ttcgcgtcca
     2701 cgcccgattc atcggtcatt tgcagcatgc cgagtgcgcg cgcgcggacc accgagccag
     2761 ccagcagcgg gaacggcgtg atgaccagca cgtcgaccgg gtcgccgtcg cccgacagcg
     2821 tttgcggaat gtagccgtaa ttcgccggat agcgcatgcc cgtgctgatg aaacggtcga
     2881 cgtggagcag gcccgtttcc ttgtcggctt cgtacttcac cggatcgctt tgcgccggga
     2941 tttcgatgat aacgttgaaa tcttgcggaa gatctttgcc tgcggggacg tgattgaagc
     3001 tcatgagcgc tctctgatca gatggaattc gaaaaacacg gcgcgcggcg cgcgggctcg
     3061 agcggcgcga atcgtaaaac tcgccagcac gaggcgctgc ggccagacag tgcggaatgc
     3121 gtcattatag ccaatcggcc gatggcgcct ctcgttggat cggcgcgata atcgactggc
     3181 gtctctcgat tgtgcctctc gtttgtgaac accgcgtagc tgaagcccgg catgaatccc
     3241 ggtctgaaca tcggctgcgc gacaccccaa ggagcatgca tggaagaagc ccggcatttc
     3301 atcggcggcg aatggtccgc tgcatcaggc ggcgagacga tcgccgtaat cgatccctca
     3361 gacggccagc cgttcaccga gctggcgcgc ggcacggcgg cggatatcga cgccgcggtc
     3421 cacgccgccc gccgcgcgtt cgaaggcgca tggggcgcgg caagcgccgc cgaacgcggt
     3481 cgcgtgctgt accgcctgtc gatgctggtc gccgcccgtc aggaggaact cgcgcaactc
     3541 gaagcccgcg acaccggcaa gccgctcaag caggcacgtg ccgactcggc agctctcgcg
     3601 cgctacttcg agttttacgc cggcgccgca gacaaactgc acggcgagac gctgccctat
     3661 caggccggct acacggtgct gacgatccgc gagccgcacg gcgtgaccgg ccatatcgtg
     3721 ccgtggaact acccgatgca gatcttcggc cgcagtgtcg gcgcggcgct cgccacgggc
     3781 aatgcgtgcg tcgtcaagcc ggcggaagat gcgtgcctct ccgtattgcg ggttgccgaa
     3841 ctggcggccg aggcgggttt gccggcgggc gcgctgaata tcgtgaccgg ttttgggcac
     3901 gaagcgggcg cggccctcgc gcgccatccc ggcatcgatc acatctcgtt caccggctcg
     3961 cccgaaaccg gcaagctcgt gacgcaaatg gccgccgaaa accacgtgcc ggtcacgctc
     4021 gaactgggcg gcaaatcgcc gcaaatcgtg ttcgccgacg ccgatctcga cgcggccttg
     4081 cccgtgctgg tgtcggcgat cgtgcagaac gccgggcaaa cctgctcggc gggcagccgc
     4141 gtgctgatcg accgggccat ttacgagccg ctgctcgacc ggctgagcgg cgcgttccac
     4201 gcgctgcggg tcggaccgtc gcatgccgat ctcgactgcg gtccgctgat caacgcgaag
     4261 cagcaacagc gcgtgtggga cttcctttcc gacgcgcaac acgacggcat cccgctggcg
     4321 gcgcacggcg aagtgatccc tgaagcaccg gaaagcggct tctatcaggc gccgacgcta
     4381 ttgcgcgacg tgccggcaag ccaccgcctc gcgcgcgacg aagtgttcgg tccggtcctc
     4441 gccgccatgc cgttcaccga cgaagacgag gcgctcgcgc tcgccaacgg cacgcagtac
     4501 ggcctggtcg ccgggatctg gacgcgcgac ggcgccaggc agatgcgcct cgcgcggcgc
     4561 ctgcgctccg gtcaggtgtt catcaacaac tatggcgcgg gcggcggcgt ggaattgccg
     4621 ttcggcggcg tgaagcattc aggccacggg cgcgaaaagg gtttcgaagc gctgtatggc
     4681 ttcacggtcc tgaaaaccat cgcgattcgt cacggctagt cagcggctcg cgtttggcag
     4741 caagcagaag cgaacgaaag tgaaaggaaa caagcgggaa caagcgcatc agcagcatcc
     4801 acaacaaaca gcggagacat tatgcggttg acaggtaaaa cggccatcgt cacgggtggc
     4861 ggctcgggtt tcggcgaagg catcgcgaag acctacgcac gcgaaggcgc gaacgtggtg
     4921 gtcaacgatc tgaacggcgc ggcggccgaa cgggtggcga gcgaaatcgc tctcgccggc
     4981 ggcaaggcga tcgcggtggc cggcgacgtc acgcaacgcg aggactggcg gacgctgcgc
     5041 gaagccgcgc tcgaagactt cggcagcgtg cagatcgtcg tcaacaatgc cggcaccacg
     5101 catcgcaaca aaccggtgct cgacgtgacg gaagccgaat tcgaccgcgt ctacgcggtg
     5161 aacgtcaaaa gcatctattg gagcgtgcag gaattcgtac cgtatttccg cgcgcaaggc
     5221 ggcggcacgt tcatcaatat cgcctcgact gccggtgtgc ggccgcggcc gggcctcgtc
     5281 tggtacaacg gcagcaaggg cgcggtgatc atcgcgagca agtcgctcgc cgtcgaactg
     5341 ggcccggacc ggattcgcgt caactgcgtg aacccggtga tcggcgagac ggcgctgctg
     5401 tcggaattca tgggcgtgga agatacgccg gaaaatcgca agcgcttcct tgcgggtatt
     5461 ccgctcggcc gtttctcgac cccgcaggat atcgccaacg cggcgctgta tctcgcctcg
     5521 gacgaggcgg agttcatcac cggcgtgtgc cttgaagtgg acggtgggcg ctgcgtgtaa
     5581 cgattgcgcg tccggcacaa ccggagccgg gcacgcggaa agcgaccggc tgcatgccgc
     5641 atcgaatcgg ttcgcctgct gcatgcgagc cggccgccgc gtgctgtgcc ctctagtcag
     5701 tgttttcccc cggtatcggt ttgccgtacc aaccaacccc tccagcacgg ctagaatcga
     5761 tcgacggcgg tacttcattt ccacaagaaa agaggagaca ccatggctag tcctgcaaat
     5821 ccgcttcacc atcccggcgc gggggcgcca ccatccactt tcgaagaggc gacctaccgt
     5881 aaggtcacct ggcggctcgc gccgctcctg atgctctgct acgtggtcgc gtatctggat
     5941 cgcgtgaacg tcggcttcgc caagctgcaa atgaccaccg acctggggtt aagcgacgct
     6001 gtttacggtt tcggcgcggg gattttcttt gtcggctatt tcatcttcga aattccgagc
     6061 aacgtgatcc tgcacaaggt gggcgcccgt gtgtggatcg cacggatcat ggtgtcgtgg
     6121 ggcgtgatct cgatgctgac catgttcgtc accacgccga ccatgttcta cgtgatgcgc
     6181 ttcctgctcg gtctcgccga agcgggtttc ttccccggca tcatcctgta tctcacgtac
     6241 tggtatccgt cgcaccgtcg tggccgcatg accacctggt tcatgacggc aatcgcgttg
     6301 tccggcgtga tcggcggccc ggtctcgggc tatatcctga agagcttcaa cggcatgaac
     6361 ggctggcacg gctggcagtg gctgtttctg ctcgagggga ttccgtcggt gatcgtcggc
     6421 gtcatggtgt tcctgatgct ggacgaccgt atctccaggg caaagtggct caccgaggaa
     6481 gaaaagcagt tactcgaacg ccaggtatcc gctgaagaag ccaccaagca cgacatgccg
     6541 atccgccagg tgctgacgag cggccgcgtg ctgatgctga gcctcacgta cttctcgttc
     6601 gtgatgggcc tgtacggcgt gagtttctgg ctgccgacca ttatcaaggc gaccggcgtc
     6661 accgactcgt tcatgatcgg cttgttgtcg gcggttccgt tcgccgccgc ggtggtcgcg
     6721 atggtgttcg tgtcgcgtag cgcggatcgc acgcgcgagc ggcgctggca tatcgcgttg
     6781 ccggccttcg cgggagcgat cggtctcgtg ctctccgtgg tgtgggccca caacaccgtg
     6841 ctggcaatgg cctcgctcac cctcgcgacc atgggcatcc tcaccacgct gccgctgttc
     6901 tggagtttgc cgacggcgat cctcgccggc acgggcgcgg cggccggcat cgcgatgatc
     6961 aattcgatcg gcaatctcgc gggctttctc agcccgtatg cggtcggttg gctcaagcag
     7021 gcgactgccg caaacgattc cggcatgtat atgctggcgg cattcatgat cctcggtgga
     7081 ctgctggcga tcagcgtgcc cgcgaagatg gtcaataagt agctggagtg tggctgaccg
     7141 atgaactcgt cgatgcgctc gcgtaggagc cgccgtcaaa cgtcctgagt gacgcgtcac
     7201 agttcatctt tttgcgagtt cacgccgcat gcacaatggt tcgtggttcg cgactcatag
     7261 tgcgcggtcc acggctcatg gctcacaccg caagccgcgc gttccaaaga aaagcccccg
     7321 gcatcttccg atgccggggg cttcttccct ttcaagctct gattcctgcg cactgcctga
     7381 gcgtttcaaa accgcgcgcc gcccccactc aaagcatcaa acgatattca tcgccaacgc
     7441 gctctcacga taatgctggc tcgccttatc cgcatcgccg agctgttcat gcaaacgcgc
     7501 caacgcgcga tgcgaacgaa tcttaagcgt ctcgttgtcg gccagtttca gcgcgcgttc
     7561 gaggaacgac tgcgccttgc cccacaactg ctgatgcaga cacaagcggc ccaatgcgaa
     7621 cagcaagtcc gcgtcttccg gacgatcctt ctgccaccct tcggccttct ggatcagcgg
     7681 cagcgcgtcg cccccggtag tatccggata acggc
//
LOCUS       DATASH010000805        10542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2706646,
            whole genome shotgun sequence.
ACCESSION   DATASH010000805 DATASH010000000
VERSION     DATASH010000805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 10542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10542
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2706646"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VHV99_21630"
     CDS             <1..201
                     /locus_tag="VHV99_21630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013338577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638032.1"
                     /translation="PPTYWRKRLYQLLDSGHLSHAQLCTVDSLLLQIDQFEAEPVWGA
                     ALATAAAALSQPAHLASASRSG"
     gene            495..1778
                     /gene="dcm"
                     /locus_tag="VHV99_21635"
     CDS             495..1778
                     /gene="dcm"
                     /locus_tag="VHV99_21635"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEX3638033.1"
                     /translation="MTLAAPLDLLRQARARFTQREIAAHVGKDIKTVRRWEKGETPCP
                     SMLEPALRDLLQSSARAKAGAATAGAQFRFIDLFAGIGGIRMGFEAHGGDCVFTSEWN
                     DFSTRTYRENYPADGEHALIGDIVAFPAEEVPSHDVLLGGFPCQPFSIAGVSKKNALG
                     RPHGFECTTQGTLFFDVARIIAAKRPAAFLLENVKNLLSHDKGRTFDVILQTLRDELG
                     YEVHYRVVDGQHFTPQHRERIIIVGFRGKTSFSWDDLRLPENGPRLGSILHRTDGSEP
                     VLPWDHDRFFDHAAKCVQPKYTLTPNLWTYLQNYAAKHRAAGNGFGFGMAYPDSVTRT
                     LSARYHKDGSEILVYQGEQLRPRRLTPRECARLMGFPDTFRIPVSDTQAYRQFGNSVV
                     MPVMREVARIMLPHVQILLAKETHHGSKQTLSLYS"
     gene            1809..2255
                     /gene="vsr"
                     /locus_tag="VHV99_21640"
     CDS             1809..2255
                     /gene="vsr"
                     /locus_tag="VHV99_21640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007587917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch endonuclease Vsr"
                     /protein_id="HEX3638034.1"
                     /translation="MVDIVDSATRSRMMSGIRGRNTKPELLIRSLLHGRGFRFRLDAR
                     DLPGRPDIVLPRYRAVILVHGCFWHGHDCHLFKWPQTRPEFWRDKIGRNRSNDDKVRA
                     ALLASGWRVGVVWECALRGANRDIEGVLQRLVEWLKSDAPSFEERA"
     gene            2357..2860
                     /locus_tag="VHV99_21645"
     CDS             2357..2860
                     /locus_tag="VHV99_21645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEX3638035.1"
                     /translation="MADFRLWSDEFPTNGFMPKAHEYHDKAFGVDGENISPALQWDAP
                     PADTQSFALTVHDPDAPTGSGFWHWVIVNIPADARSLPRNAGKADGSLLPQGALQVRN
                     DYGTVGFGGAAPPRGDKAHRYIFRLHALKVPHLPINADTTNAVARFMTHLNELDSTTH
                     TGLYELK"
     gene            2999..4453
                     /locus_tag="VHV99_21650"
     CDS             2999..4453
                     /locus_tag="VHV99_21650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3638036.1"
                     /translation="MHAQPSRKNGSTPGNAASGAPQGTGTPFADSTEQGLPIPQRYWA
                     MLVIALGLTLAVLDSAIANVALPTIARNLHASAAGSIWVVNAYQLAITISLLPLASLG
                     DRIGYRRIYLAGLILFTVASFGCALSTSLPTLALARVVQGFGAAGIMSVNTALVRMIF
                     PPAQLGRGVAINAMVVAVSSAVGPTVASGVLAVASWPWLFAINVPIGIAAIVGGFKAL
                     PMNPGHESPYDSLSAVMNAFVFGLLIFAVDGLGHGERLGWVVAEGLGAIVIGYFFVRR
                     QLTQSAPLLPIDLLRIPVFALSIGTSVCSFCAQMLAFVSLPFLLQDTLGFSQVDTGLL
                     MTPWPAVIIIAAPIAGVLADKVSSGWLGGVGLVAMTVGLLLLATLGAHPEPMQIAWRM
                     AVCGAGFGIFQSPNNRTILSAAPRERSGGASGMLGTARLTGQTLGAALVALIFGIAPQ
                     HGPTVVLYVAACFSAVAAVVSTLRVMQRVNVQAA"
     gene            complement(4594..6021)
                     /gene="otsA"
                     /locus_tag="VHV99_21655"
     CDS             complement(4594..6021)
                     /gene="otsA"
                     /locus_tag="VHV99_21655"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase
                     (UDP-forming)"
                     /protein_id="HEX3638037.1"
                     /translation="MSRLIVVSNRVAPTQEGRPSAGGLAIGVLDALKETGGVWFGWSG
                     ETVNEPSPPVIEKQGNVTYATVGLTKRDYDQYYRGFSNATLWPTFHYRNDLSRYDRQE
                     YAGYQRVNATLAKQLKALLKPDDIIWVHDYHLLPFARCLREFGVKNPIGFFLHIPFPV
                     PEVLRTIPPHEELVKAMCSYDVVGFQTEADRQSFVDFIERGQHGTSSEDGMVHAYNRF
                     LKVAAYPIGIYPDAIAKAAEQFTDRKPVRSLRDGMRGRKLIMSVDRLDYSKGLVERFQ
                     AFERLLLNAPGWHGRVSLVQIAPPTRADVQTYQRIRQTLEGEAGRINGRFAQLDWTPI
                     QYLNRKYERNLLMALFRQSQVGYVTPLRDGMNLVAKEYVASQDPADPGVLVLSQFAGA
                     AEQLPGALVVNPFDLSQMAEALERALSMPLAERQARHADMMAPMRENNLSVWRDTFLG
                     DLRNVATASSVTAKAVKLADVTASS"
     gene            complement(6232..6459)
                     /locus_tag="VHV99_21660"
     CDS             complement(6232..6459)
                     /locus_tag="VHV99_21660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638038.1"
                     /translation="MRHPALRRSARHSKRDSRPASSKNGPPEAQDPSAHDPSAQNRVA
                     PDAPPDGEYNQGGVRREGLEYQRDLGSEQDA"
     gene            complement(6613..6861)
                     /locus_tag="VHV99_21665"
     CDS             complement(6613..6861)
                     /locus_tag="VHV99_21665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638039.1"
                     /translation="MPEYTPAPALPLSKHAHRPEPIEPPSPSPAPDPGEPDTIPSPSP
                     TPDPIEPVAPPIGDPPPQPTQTPQASPRPPSHRSSMQL"
     gene            complement(8030..9430)
                     /locus_tag="VHV99_21670"
     CDS             complement(8030..9430)
                     /locus_tag="VHV99_21670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX3638040.1"
                     /translation="MPHVLIVDDDAGTREALSAIIGEDGLTTATAGDLREARIQLVRQ
                     MPDVVFTDLKLPDGSGVDLFEDLDPRSGVEVIVITGHATVESAVNALKMGATDYLVKP
                     INMQRVKAILSRLPRAGDLKAEIGTLRGELRRMGRFGLMLGNSPAMQEVYDQIGRVAP
                     TAASVMLVGESGTGKEVAAQTLHQLSLRRKHAFLAVNCGAISPNLIESEMFGHERGSF
                     TGADRQHKGYFERANGGTLFLDEITEMPIELQVKLLRVLETGMFMRVGTTKEIETDVR
                     LIAATNRDPEQAVLEGKLRLDLYHRLNVFPISLPPLRDRGKDVELLAQAFLDELNERH
                     STKKHFPAAVKDMLMSYPWPGNVRELKNYVQRAHIMSGSDSDSTATVPLQITLSKPAA
                     GTAITIPFGTSLAEADRQLILATLEQCGGVKTRAAEILGISLKTLYNRLVEYGNDARD
                     EGDAADEPRALKGADA"
     gene            complement(9749..10027)
                     /locus_tag="VHV99_21675"
     CDS             complement(9749..10027)
                     /locus_tag="VHV99_21675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638041.1"
                     /translation="MGRPKIGIFGALMALGGFLAVRYVQRNRAAQIPAARELSRWEGE
                     GGAVVAPATPSGSVTAASAGPSTPDRADGANGSAHPNGHGGAWPFPRS"
     gene            complement(10078..10395)
                     /locus_tag="VHV99_21680"
     CDS             complement(10078..10395)
                     /locus_tag="VHV99_21680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HEX3638042.1"
                     /translation="MTDTTQQLALGGQKIVDDLRVLLTDSEEMLRLAANVPGEGVDAL
                     RNRLGAHVETLQSTLGDAQRNAQRRYRAATVNTERYVRRNPWRSIGIAAGVGFLLGVL
                     SAR"
BASE COUNT         1949 a         3355 c         3176 g         2062 t
ORIGIN      
        1 ccccccacct actggcgcaa gcgcctatat cagttgctcg actccggcca tctgtcccac
       61 gcgcaactgt gcacggtcga cagcctcctg ctccagatcg accagttcga agccgaaccc
      121 gtgtggggcg ctgcgctggc cacggcagcc gccgcgctgt cccagccggc gcacctcgcg
      181 agcgccagcc ggtctggctg agacgctgca ggccttgatg gccggccttc cacgtcggca
      241 gtctcactgt ttcccagcta aaaaaaggcc gcgaaccctg tcgcggccgg aacacgtcgc
      301 aacgcgggga attcgcgtca cactggcagc ctacacgtcc aactgtgaca accgcattta
      361 ggccaactgt ggcaccgcat ttaggcgaat gccgttttgg cttatcgcaa attttctgcc
      421 cgtcgacggc aaaaatagga aaaagggacg accttaggtc ataatgtccg caaaaattcc
      481 cagcaaggac ctccgtgacc ctcgccgcac cgctcgatct gctcagacag gcgcgcgccc
      541 gcttcactca acgcgaaatc gccgcgcacg tcggcaagga catcaaaacg gtacgccgct
      601 gggaaaaagg cgaaactccc tgtccgtcca tgctcgaacc ggccttgcgc gatctgctgc
      661 agagcagtgc ccgggccaag gctggggcgg ccacggccgg cgcccagttc cgcttcatcg
      721 atctgtttgc cggcatcggc ggcattcgca tgggttttga ggcacacggc ggcgactgcg
      781 tcttcacgag cgagtggaat gatttctcca ccaggaccta tcgcgagaac tacccggccg
      841 acggcgagca cgcgctgatc ggcgacatcg tcgcgtttcc cgccgaagaa gtgccgagcc
      901 acgacgtgct gctcggcggc tttccgtgcc agccgttctc gattgccggc gtcagcaaga
      961 aaaacgcgct gggccgcccg cacggcttcg aatgcacgac gcaaggcacg ctgtttttcg
     1021 acgtcgcgcg catcatcgcg gcgaaacggc cggccgcgtt cctgctggag aacgtgaaga
     1081 atctgctctc gcacgataaa ggccgtacct tcgacgtgat cctgcagacc ctgcgcgacg
     1141 agctcggcta tgaagtgcac taccgtgtgg tcgacggcca gcatttcacg ccgcaacatc
     1201 gcgagcgcat catcatcgtc ggctttcgcg gcaagacgtc gttttcctgg gacgatctgc
     1261 gtctgccgga gaacggcccg cgcctcggct cgatcctgca tcgcacggac ggcagcgaac
     1321 cggtgctgcc ctgggaccac gatcgcttct tcgaccacgc cgccaaatgc gtgcagccga
     1381 agtacacgct cacgccgaat ctctggacct atctgcagaa ctacgcggcg aagcatcgcg
     1441 cggcaggcaa tggcttcggt ttcggcatgg cctatcccga cagcgtcacg cgcacgctct
     1501 ccgcgcgtta tcacaaggac ggctcggaga ttctggtcta tcagggcgaa cagttgcggc
     1561 cacggcgcct cacgccgcgc gaatgtgccc ggctgatggg ctttcccgat accttcagga
     1621 ttccggtcag cgacacccag gcttaccggc aattcggcaa cagcgtcgtc atgccggtga
     1681 tgcgggaagt ggcgcgcatc atgctgccgc acgtgcaaat cctgctcgcc aaggaaacgc
     1741 accatggttc gaagcaaacc ctttcgctct attcctgagc agcctgattc attacccagg
     1801 cgctaacgat ggtcgatatc gtcgacagcg caacgcgtag ccggatgatg tccggcattc
     1861 gcggccgcaa cacgaaaccg gaactcctga tccgcagcct gctgcatggc cgcggctttc
     1921 gcttccgtct ggacgcgcgc gatctgccgg gccgcccgga tatcgtgctg ccgcgctacc
     1981 gcgcggtgat tctggtccac ggctgcttct ggcacggtca cgactgtcac cttttcaaat
     2041 ggccgcaaac gcggccggaa ttctggcgcg acaagatcgg ccgcaatcgc agcaacgacg
     2101 acaaggttcg ggcggccttg ctcgcgagcg gctggcgcgt cggggtggtg tgggaatgcg
     2161 ctttgcgggg tgcgaatcgc gatatcgaag gcgtcttgca gcggctcgtc gagtggttga
     2221 agagcgacgc gccgagcttc gaggaacgcg cttgaaagct gcgtgtcgtc tgtccgatgc
     2281 gagggaatct cgaagccgct ggtgatacac tcgatcagct aaccatccca gaaccgctca
     2341 cgaagggagg cacacaatgg ctgatttccg tctctggtcc gacgagttcc ccaccaacgg
     2401 ctttatgccg aaagcccacg agtatcacga caaggctttc ggtgtggacg gcgagaatat
     2461 ctcgcccgcg ctgcaatggg acgcaccgcc ggccgacacc caaagcttcg cactcaccgt
     2521 gcacgatccc gacgcgccca ccggcagcgg cttctggcat tgggtgatcg tcaatattcc
     2581 ggccgatgcc cgctcgctgc cgcgcaatgc cggtaaggcc gacggctcgc tgctgccgca
     2641 aggcgcgctg caagtacgca acgattacgg cacggtcggc ttcggtggcg ccgcgccgcc
     2701 gcgcggcgac aaggcgcatc gctacatctt ccgcctgcat gcgctgaagg tgccgcatct
     2761 gccgatcaat gcggacacca ccaatgcggt cgcacgcttc atgacgcatc tgaatgaact
     2821 cgattcgacc acccacacgg gtctgtacga gctcaaataa cgcgtaagat ccgatgccag
     2881 cgcgctgttg cggcgccggc atcggcgccg cgcgccgtct tgtctttcga gagcaatagg
     2941 ccgcgcgcgc ggttcatcgg cgggtcgatg agccgcctca ctccaaatca aaaaaacgat
     3001 gcacgcacaa ccatcgcgca agaacggttc gacgccgggc aatgccgctt caggcgcccc
     3061 ccagggcacc ggcacaccgt tcgcggatag caccgaacaa ggcttgccga tcccgcaacg
     3121 ctactgggcc atgctggtta tcgcgctcgg cctcacgctg gcggtgctcg acagcgcaat
     3181 cgccaacgtc gcgctgccga ccatcgcgcg caatctgcac gcgagcgcag ccggctcgat
     3241 ctgggtcgtc aatgcttacc agctcgccat caccatttcg ctgctgccgc ttgcgtcgct
     3301 cggcgaccgt atcggctatc ggcgcattta tctggccggg ctgatcctgt tcacggtggc
     3361 gtcgttcggt tgcgcgctgt ccacctcgct gccgacgctc gcgctcgccc gcgtggtcca
     3421 gggtttcggc gcggcgggca tcatgagtgt caacaccgcg ctcgtgcgca tgatctttcc
     3481 gcccgcgcag cttgggcgcg gcgttgcgat caacgcgatg gtcgttgcgg tgtcgtccgc
     3541 ggtcggaccg acggtcgctt ccggggtgct cgccgtcgcc tcgtggccgt ggctgttcgc
     3601 gatcaatgtg ccgatcggga tcgcggcgat cgtaggcggc ttcaaggcgc tgccgatgaa
     3661 tcccggccac gagtcgccct acgactctct gagtgcggtg atgaacgcgt tcgtgttcgg
     3721 cctgctgatt tttgccgtcg atggtctcgg ccacggtgag cgcctcggct gggtcgtcgc
     3781 ggagggactc ggtgcgatcg tgatcggtta tttcttcgtg cgtcgtcaat tgacccagtc
     3841 cgcgccgctg ctgccgatcg atttgctaag gattccagtg ttcgcgctgt cgatcggtac
     3901 ctcggtgtgt tcgttttgcg cgcagatgct ggccttcgta tcgctgccgt ttctcttgca
     3961 ggacacgctc ggtttctcac aggtcgatac cggcttactg atgacgccgt ggccggcggt
     4021 gatcatcatc gccgcgccga ttgcgggtgt gttggcggac aaggtgtcgt caggctggct
     4081 cggcggcgtc ggacttgttg cgatgaccgt cggcctgttg ctgctcgcca ccctcggcgc
     4141 acatcctgag ccgatgcaga tcgcgtggcg catggcggtg tgcggtgcgg gcttcgggat
     4201 cttccagtcg ccgaacaacc gcaccatcct gtcggccgcg ccacgcgagc gcagcggcgg
     4261 agcgagcggc atgctcggca ctgcgcgcct gaccggacag acgctcggcg cggcactggt
     4321 cgcactgatc ttcggcatcg cgccccagca tgggccgact gtcgtgctgt atgtcgccgc
     4381 gtgcttttca gcagtcgccg ccgtggtcag cacgctgcgg gtgatgcagc gcgtgaatgt
     4441 gcaagcggcg tgagcgtgtt gcgagcaatg caacaagggc gcgaatcgca tggagcggtc
     4501 cgcgcccttc tttaatgctt cattcaaccg caaatcgttc gacgtgagca gcgcgcgcgg
     4561 cctccgaaaa aatccgcgcc gccactgagg ccgttacgac gatgccgtca cgtcagcgag
     4621 ctttaccgct ttggccgtca cggaagaggc agtcgcgacg ttgcgcaaat cgcccaggaa
     4681 cgtatcgcgc cagaccgaca gattgttctc gcgcatcggc gccatcatgt ccgcatgccg
     4741 cgcttgccgt tcggcgagcg gcatcgacag tgcgcgctcc agcgcctcgg ccatctgcga
     4801 cagatcgaac ggattgacga ccagtgctcc cggcagttgc tcggccgcgc cggcgaattg
     4861 cgacagcacg agtacacccg gatcggccgg atcctgcgag gcgacgtact ccttcgcgac
     4921 cagattcatc ccgtcgcgca gcggcgtcac gtaaccgacc tgcgattgac ggaacagcgc
     4981 catcagcaga ttgcgctcgt atttgcggtt cagatattga atcggcgtcc agtcgagttg
     5041 cgcgaaccgg ccattgatgc ggccggcttc gccctccagc gtctggcgga tgcgctgata
     5101 ggtctgcaca tcggcgcgag tcggcggtgc gatctgcacc agcgatacac ggccgtgcca
     5161 acccggcgca ttcaacagca accgctcgaa cgcctgaaag cgctccacga ggcccttcga
     5221 ataatcgagc ctgtcgacgc tcatgatcag cttgcgccca cgcatgccgt cacgcaggct
     5281 gcgcaccggc ttgcggtcgg tgaactgctc agcggctttg gcgatcgcgt ccggatagat
     5341 gccgatcggg tacgccgcca ctttcagaaa gcggttgtac gcgtgcacca tgccgtcctc
     5401 gctcgaggtg ccgtgctggc cgcgctcgat aaagtcgacg aacgactgac ggtccgcctc
     5461 ggtctgaaag ccgaccacgt cgtagctgca cattgccttg accagctctt cgtgcggtgg
     5521 aattgtccgg agtacttccg gaactggaaa cggaatatgc aggaagaaac cgattggatt
     5581 tttcaccccg aactcacgca ggcagcgcgc gaacggtagc aggtgatagt cgtgcaccca
     5641 gatgatgtca tcgggtttga gcagcgcctt gagctgcttg gcgagcgtcg cgttgacccg
     5701 ctgatagccc gcgtattctt gccgatcgta gcgcgacagg tcgttgcgat agtggaaggt
     5761 cggccacagt gtcgcgttgg aaaagccgcg atagtactgg tcgtagtcgc gcttggtgag
     5821 gcccaccgtc gcgtacgtca cgttgccctg cttttcgatc accggagggg acggctcgtt
     5881 caccgtctcg ccgctccagc cgaaccagac tccgccggtt tccttcaacg cgtccaacac
     5941 gccgatcgcg aggcccccgg ccgagggacg gccttcctgc gtcggcgcga cacgattcga
     6001 taccacgatc agtctgctca ttggtgctcc atctggtcag ttgtttcgtt gcgtacggat
     6061 aagtgccaaa aagaggtatg caaatcgcgt gccgtttttt gagatgttgg gaataaaaat
     6121 gtgtgcaaat gtgacggacg agcagaaacg cgcgactcag tagcgccacg aagaattttt
     6181 tgctgcttta catggcaaaa ccgggaccat tcttaccgct cgaccaaccg cttaagcgtc
     6241 ctgttccgag ccgagatcgc gttgatactc cagtccttcg cgacgcacac cgccctggtt
     6301 gtactcgccg tctggcggcg cgtcgggcgc gacgcggttt tgcgccgagg ggtcgtgcgc
     6361 cgaggggtct tgcgcttcgg gcggaccgtt cttgctgctg gccggccggg aatcgcgttt
     6421 cgagtggcgc gcggaacggc gcagtgctgg atgtctcatg atcgatgcct ccatgggaag
     6481 cccgccgcta tcgggaacgg catcaataca gtctagtggt cgcgacggta aattgccggt
     6541 aaaagatgcg ccggctttgt aacaagtaca taaaattgct gcgccggtat gtggcgcggc
     6601 gcacagaacg cgtcagagtt gcatgctcga tcgatgcgaa ggcggacggg gactggcttg
     6661 tggcgtttgc gtgggctgtg gtgggggatc gccgatcggc ggcgcaacgg gttcgattgg
     6721 gtcgggagtc ggtgaggggc ttggaatggt gtcgggttcg ccggggtcgg gtgccggcga
     6781 tggcgatggc ggctcgatcg gctctgggcg gtgagcgtgc ttcgagaggg gaagcgcggg
     6841 cgcgggggtg tattccggca tgttagttat cctccgtttt ttgctgtcct ctggagggca
     6901 gcaaggcttg tgccggtggg gtttttgcct tcgggctttt ttgtagatgg gtttgccatc
     6961 gcggcgctct ttgtgtgatt gcctcaggca ttgggttttt gcttgattta tttacctgct
     7021 cagggcgttt tcgctggttg cctgcggcgg tggcctttcc ttgatttgtt attggtttat
     7081 tagcgttgcc cctgtgcggg gcggcaccta cttttctttg cctgccgcaa agaaaagtag
     7141 gcaaaagaaa gcggctcaca ccgctagcgc ataagcgggt ccctcgcgca gttgcggtag
     7201 tggtgcatct gaaatctgtg ccctcgcgca ttcagcgtga gtgacaaggc gctcatactt
     7261 cccgcctcgc cctgcgtgct cgccggaagg gttcatcatg caaaccagtg gcttcgtttc
     7321 ggggcagtgg gccatcggct tcgcctcggc gagacgctga aaataccaga tagctttctg
     7381 ggtgccccgc caagcgcctc aacgactagc ggccgcgctg gggcacccaa ccaaaggaag
     7441 caactattgg cctaatgaag cagcagagaa cgataataac cgttggtttt actaagtttc
     7501 cacggagcct taaccataga gcacggttac cgacctgcgc aaagacttag caacagtgca
     7561 aggttcgctc aactgccgcc gagcggcaac aaaagtacat gtttgctgaa ccgccatcga
     7621 gcctcagcaa cagtgcatgg tttgctgaat tgccgctgag tgtcgacaac aacacacgtg
     7681 tttgatcaag taccgctacg gcgcgcgcag cgtcgccgga agcatgactg ctgtgtcact
     7741 gacgccgaat gtgcgagggc acggattcca gatgcgccac taccgtgact gtgcggggga
     7801 cccgcttatg cgctggcggt gtgagccgct ttcttttgcc tacttttctt tgcggcgggc
     7861 aaagaaaagt aggtgcccgc cccgcacagg ggcgacgcta atagaccact aacaattcaa
     7921 ggaaaggcca aaacgccaaa tcaaggaaaa ggccaaaaag ccagttcaac caaagaccaa
     7981 cgccgcaggc aaaacaaccc aaacccaccg ccggaggcaa aaaaaaccct cacgcatcag
     8041 cccccttcaa agcccgcggc tcatcagccg catccccctc atcacgcgca tcattcccat
     8101 actcaacaag ccggttatac aaagtcttaa gactaatacc aagaatctca gcagcccgcg
     8161 tcttcacccc accgcattgc tcaagcgtcg ccagaatcaa ctgccggtcc gcttcggcaa
     8221 gcgaagtccc aaacggaatc gtgatcgcag tacccgccgc cggcttcgac aatgtgatct
     8281 gcaacggcac cgtcgccgtg ctgtccgaat ctgaacccga catgatgtgc gcgcgctgca
     8341 cgtagttctt caactcgcgc acattacccg gccagggata cgacatcagc atgtccttca
     8401 ctgcggccgg gaaatgcttc ttcgtgctgt gacgctcgtt gagttcgtca agaaacgcct
     8461 gcgcaagcaa ctccacatcc ttaccccgat cgcgcaacgg cggcaggctg atcggaaaca
     8521 cattcagacg atggtacaaa tcgagccgca acttgccctc cagcaccgcc tgctcaggat
     8581 cgcggttggt cgccgcgatc agtcgcacgt ccgtttcgat ttccttggtc gtgccgaccc
     8641 gcatgaacat gccagtttcc agcacacgca gcaatttcac ctgcaactca atcggcattt
     8701 cggtgatttc gtcgaggaac agcgtgccgc cgttcgcgcg ctcgaaatag cctttgtgct
     8761 gacggtccgc gccagtaaac gagccgcgct cgtgaccgaa catttccgac tcgatcaggt
     8821 tcggcgagat cgcaccgcag ttcaccgcga ggaacgcgtg tttccggcgc aagctgagtt
     8881 ggtgcagtgt ctgagcggca acttccttgc cggtccccga ctcgcccacc agcatgaccg
     8941 aggccgccgt cggcgcaacg cggccaatct ggtcgtagac ctcctgcatg gccggtgaat
     9001 tgccgagcat caagccaaaa cggcccatac ggcgcaactc gccgcgcaac gtgccgattt
     9061 ccgccttcag gtcgccggcg cgcggcagtc gcgagagaat cgccttcacg cgctgcatgt
     9121 tgatcggctt caccaggtag tcggtggcgc ccatcttcag cgcgttgacc gccgactcca
     9181 ccgtggcatg gcccgtaatc acgatcactt ccacgccgga gcgcggatcg agatcttcaa
     9241 acagatcgac tccgctgccg tcgggcagct tcagatcggt aaagacaacg tccggcatct
     9301 gccggaccag ttgaatgcgc gcttcgcgca aatcgcccgc agtggcggta gtcagaccgt
     9361 cttccccgat gatggcggaa agcgcctcgc gggtaccggc atcgtcatcg acaatcagta
     9421 cgtgtggcat cgcgtctcga aagcccgaaa gcgtgaatga ggaagcatgc gaaaccgcat
     9481 gctgaaaaaa gccggccggc ggcttgccgc tgcgccgcga tgcgcccgga tgtgagcaca
     9541 cgccaacgtt tttacaactg cgtccaaccc tcgacttaat ccaatattgc aacaatcaac
     9601 agggaaatcg cccaatggac tgcgacattg ttgtctattt acctattata cggctttaac
     9661 gagataaatt tgtcatgcgc cccaaacgcc gccgtcaaag tttcacaccg gcgacgcggc
     9721 gcgcgcaagg ctctttcaag tgccgtgctt aactgcgtgg aaacggccat gcgccgccgt
     9781 gtccgttcgg atgcgccgag ccattcgcgc catccgccct gtccggcgtg gacgggccgg
     9841 cactcgccgc cgtcacgctg ccggacggcg tagcgggcgc caccactgcg ccgccctcgc
     9901 cttcccagcg gctcaattca cgcgccgccg gaatctgcgc ggcgcggttt cgctgcacgt
     9961 agcgcaccgc cagaaacccg ccgagggcca tcagcgcgcc gaagatgcca attttgggtc
    10021 gtcccatcgt gaactccttt gtttatcgtc gagcggatcg gcgtcgagca ttgcgcgtta
    10081 gcgcgcgctc agcacgccaa gcagaaagcc gacgcccgcg gcaatgccga tcgagcgcca
    10141 cgggttgcgc cgcacgtagc gttccgtatt gacggtggcg gcccggtagc gccgctgcgc
    10201 attgcgctgg gcatcgccaa gcgtcgattg cagggtttcg acatgcgcgc ccaaacggtt
    10261 gcgcagcgcg tcgacgccct cgcccggcac gttggcggca agccgcagca tctcttccga
    10321 gtccgttagc agcactctca gatcatcgac aatcttctgg ccgccgagtg caagctgttg
    10381 cgtggtgtca gtcatcattc gctcctatgt ctgaattcgg cgtttgcgtg ttatttcggg
    10441 tgcggcgcga accatcggaa ccgtatccgt gccgcgcgct tcgaaccagg acgttcaaaa
    10501 gcacacgcgt gtcgggttga cggcgcaaac cccgtgcccg gt
//
LOCUS       DATASH010000806         2816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_135290,
            whole genome shotgun sequence.
ACCESSION   DATASH010000806 DATASH010000000
VERSION     DATASH010000806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135290"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="VHV99_21685"
     CDS             <1..785
                     /locus_tag="VHV99_21685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="catalase-related domain-containing protein"
                     /protein_id="HEX3638043.1"
                     /translation="ERVEGTKIRVRSESFADHFSQAALFYNSMSQPEKDHIAAAYQFE
                     LGKVTKPEIRARVVNEILANFDAGLAAKVAEGLGLPAPKKGTAKLTGPKESPALSLLN
                     RVKPGIKTRKIALLAAPGSDGAAIRKLQQALKGEGAAPMLIAPTLAAIDGMAPDATIA
                     GLPSIMFDAVIVVGGEAGAKTLAQSGDARHFVLEAFKHLKALAAIGAGRDVLAAAHLP
                     DHADGVVSGDDKQAADVLKNFIKVAEQHRVWSRAAQAETVPA"
     gene            complement(914..2725)
                     /gene="treA"
                     /locus_tag="VHV99_21690"
     CDS             complement(914..2725)
                     /gene="treA"
                     /locus_tag="VHV99_21690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014971828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreA"
                     /protein_id="HEX3638044.1"
                     /translation="MTYPILTTLSRTRLRSALVAAVLWYASFAGAVLPIAPAVGGGIA
                     SAAMHVAPPTQASPVVAVPPSEQYPALYRDVELAHLFPDSKTFADMVPLSSPARITAA
                     YEAAKAQAGFSLGDFVKRNFVLPTRATKAYVSDPNQDVVSHIDTLWNVLRREPDSTAS
                     PWSSLLPLPYAYIVPGDRFDEIYYWDSYFIMLGLEQSGQYALVVDELKNFATLIDRYG
                     HIPNGNRTYYLSRSQPPFFAQMVRLVAEKDGDAVYVQYLPELQREYAYWMDGSDGLPA
                     GQASRHVVRLADGSLLNRYWDERAAPRDESYREDVVTAHQTPQRDSADLWRNLRAGGE
                     TGWDFSSRWFADGKTLATVDVTSLVPVDLNCLLVDLERTLAKAYRLRGDVTHAENMEQ
                     RATARADAIRRVLWDPQLQAFGDYDFVHRTLTHRLTAATVYPLYTGVASRQQAKTVAA
                     TVQHELLRPGGLATTQVNSGQQWDAPNGWAPLQYLAVIGLRRYSEPVLAQTIATRWIR
                     TNVSYYQHTGKLVEKYDVDAAAPGVAAGGGEYPLQDGFGWTNGVLRALLVLYPQTVQA
                     SRPSDIPEGPAGVSAAAASAAAAPKNTHRLHETRVNP"
BASE COUNT          557 a          821 c          926 g          512 t
ORIGIN      
        1 aggagcgcgt ggaaggcacc aaaattcgtg tgcgcagcga gtcgtttgcc gatcattttt
       61 cgcaggccgc gctgttttat aacagcatgt cccagcccga aaaagatcat atcgccgccg
      121 cgtatcagtt cgaactcggc aaagtcacca agccggagat tcgcgcgcgc gtggtgaacg
      181 agattctcgc taacttcgat gcggggctcg cagcgaaggt cgccgaagga ctcggcttgc
      241 ccgccccgaa gaaaggaacg gcgaagctca cggggccgaa ggagtcgccg gcgctcagtt
      301 tgctcaatcg cgtgaagccc ggcatcaaga cccgcaagat cgccttgctg gccgcgcctg
      361 gttccgatgg cgcggcgatc aggaagctgc aacaggcgct gaaaggcgag ggcgcggcgc
      421 cgatgctgat tgcaccgacg cttgccgcca tcgacggcat ggcgcctgac gcaaccattg
      481 ccgggttgcc gtcgatcatg ttcgacgctg tgattgtggt gggcggcgaa gcgggcgcga
      541 agacgctggc gcaatcgggc gatgcacggc acttcgtgct cgaagccttc aaacatctga
      601 aggcgcttgc ggcgatcggc gcggggcgcg acgtgctggc cgcggcccac ttgccggacc
      661 atgccgacgg cgtggtcagc ggcgacgaca agcaggcggc cgatgtgctg aagaacttca
      721 tcaaggtggc tgagcagcat cgcgtgtggt cgcgtgccgc gcaggcggag acagtgccgg
      781 cgtaatgacg gtatgacttg atcatcaggt ttgtctgagc attgcggctc ttcaagcagc
      841 cgcgttgctt aagcggactg gccggtgtga accggccagt tttttttgtg tgctgaaatg
      901 atgaaaccga cggttacgga ttgactcgcg tctcatgcag ccgatgcgta ttcttcggtg
      961 ccgcggcagc ggaagccgcg gccgccgaca ctcccgcggg gccttcagga atatcgctgg
     1021 gccgcgaggc ctgcaccgtt tgcggataga gcacgagaag ggcgcgcaac accccattgg
     1081 tccaaccgaa gccatcttgt agcggatact cgccaccgcc agcagcgaca cctggtgcgg
     1141 ctgcatcgac atcgtacttc tcgaccagtt tgccggtgtg ctgatagtag gagacgttgg
     1201 tcctgatcca gcgcgtggcg atggtttgcg cgaggaccgg ttcgctatag cggcgcaagc
     1261 cgatcactgc cagatactgc agaggtgccc aaccgttcgg tgcatcccat tgttggccgc
     1321 tattgacctg cgtggtggcg agcccgccgg ggcgtagcaa ttcgtgctgc acggtggcgg
     1381 cgacggtctt cgcctgctga cggcttgcta cacccgtata caacggatag accgtggcgg
     1441 cggtgagacg gtgcgtgagt gtgcgatgca cgaagtcata gtccccgaac gcttgcagtt
     1501 gcgggtccca cagcacgcgc cggatggcgt ccgcgcgcgc ggtggcgcgc tgctccatat
     1561 tttccgcatg cgtgacatcg ccgcgcagac ggtacgcttt cgccagtgtg cgttccagat
     1621 cgaccagcag gcaattcagg tcaacgggca cgagcgaggt cacgtcgacg gtggcgagcg
     1681 tcttgccgtc tgcgaaccat cgcgagctga aatcccaacc ggtttcaccg cccgcgcgca
     1741 gattgcgcca cagatcggcg gagtcgcgtt gaggcgtttg gtgcgcggtg acaacgtctt
     1801 cgcgatacga ctcgtcacgg ggcgccgcgc gctcgtccca atagcggttg agcagcgagc
     1861 cgtcggcgag gcgcactaca tgccggctag cctggcctgc cgggaggcca tcgctgccat
     1921 ccatccagta ggcgtattcg cgctgcaatt caggcaggta ctgcacatag acggcgtcgc
     1981 cgtccttctc ggcgacaagg cgcaccatct gcgcgaagaa cggcggctgc gaacggctca
     2041 gatagtaggt gcgattgccg ttcggaatat gcccataccg atcgatcagg gttgcgaagt
     2101 ttttcaattc gtcgacgacc agcgcatatt gtccgctctg ttccaggccg agcatgatga
     2161 agtacgaatc ccagtaatag atctcatcga aacgatcgcc cggcacgata tacgcgtagg
     2221 gcagcggcaa cagtgacgac cacgggcttg ccgtggaatc cggttcccgg cgcaggacat
     2281 tccagagcgt atcgatatga ctgaccacat cctgattcgg atcggagacg taggcctttg
     2341 tcgcgcgagt ggggagtacg aaattgcgct tcacaaaatc gccgagactg aaaccagcct
     2401 gcgcctttgc tgcctcataa gcggcggtga ttcgcgccgg cgacgaaagc ggcaccatat
     2461 cggcgaaggt tttgctgtct gggaagagat gagcgagttc tacatcacga tacaacgcgg
     2521 ggtactgctc ggacggcggc acggccacga cgggcgacgc ttgcgtgggc ggcgccacgt
     2581 gcatggcggc acttgcgatg cccccgccga cggctggcgc aatcggcaga acggccccgg
     2641 caaaagacgc gtaccacagg accgcagcga caagtgcgga acgaagacga gtacgagaga
     2701 gcgtggtgag aatgggatag gtcatcggcg acgggcaaga gtggggacgt aaacgatgaa
     2761 ggacagctat tcttctcacg attgactcgc gttagcaacg caaagttgcg cacaat
//
LOCUS       DATASH010000807         1746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4089260,
            whole genome shotgun sequence.
ACCESSION   DATASH010000807 DATASH010000000
VERSION     DATASH010000807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4089260"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VHV99_21695"
     CDS             complement(<1..363)
                     /locus_tag="VHV99_21695"
                     /inference="COORDINATES: protein motif:HMM:NF013577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEX3638045.1"
                     /translation="MNGILSRVRELQPDLPPKAKQVTELIIAQPESFIHMSITEVAET
                     CGVSEGTIVSFCRRVGVRGFQELKILLARDLIEPVRLIQEDLHRGDDPATVTDHIFAA
                     HAASLHETRRLLSMESLAK"
     gene            917..>1746
                     /locus_tag="VHV99_21700"
     CDS             917..>1746
                     /locus_tag="VHV99_21700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX3638046.1"
                     /translation="MTTRRDFLAGATAAAAFASIRQALAIPARRRTGTIKDVKHIVIL
                     MQENRSFDHYFGTLRGVRGFGDRFPIPLESGKSVWFQSDGTREITPYHRDSATSNALV
                     GYGTPHSFGDSQAAWNQGKMGLWAKYKTPYAMGYFGRDDIPFQFALAEAFTLCDAYHC
                     SITTGTDPNRITFWSGSNFNPALRAQGINCTTNDSEPNNSRCWPSPSKWVAGLPQPQP
                     TYKYVNSDFAWDTLPDLLQRAGVSWHIYQDMNDNWTGAMHGCLAFSSFRHAQPGSPNY
                     V"
BASE COUNT          345 a          569 c          500 g          332 t
ORIGIN      
        1 cttcgccaac gattccattg agagaaggcg gcgcgtctcg tggagcgaag cggcgtgggc
       61 ggcaaagatg tgatcggtga cagtcgccgg atcatcgccc cgatgcagat cctcctgaat
      121 cagacgaacc ggctcgataa gatcgcgcgc cagaagaatc ttcagctcct gaaatcctcg
      181 aacgcccacc cggcggcaaa aggaaacaat cgtgccttcg ctgacgccgc atgtctcggc
      241 aacctcggtg atcgacatgt gaatgaaaga ctcgggctgc gcgataatca gctcggtgac
      301 ttgcttggct ttcggaggaa gatcgggctg caattcacga acgcgcgata ggataccgtt
      361 cacggacggt cctgcactga agttcgatcg tgtcacccgc gtcctcgcga acaacgtagc
      421 aattcattgg gtcgatcgcc gggaacatga aaatgcggtg ccgattgcaa tgtcaatagc
      481 ttgtcgcctg gcgcgtacgg cacgctagcg gcgcttaccc acgctcttcc gttgcgatcg
      541 cttcggtacg cacgtgaagc cgtcattttc acacagccgt cgttttcatt ttgaatagtg
      601 gtgccgcgcg acgaggccgg gcttggtcgg cgcctcgagt tttgcgcgaa gcttgtcgcg
      661 cgcgagcggc ggaacattcc ggcgtcggca cgctccctcc atttcgagac actctcgctg
      721 gctctctgtt cgggcgctca acgtatactg agaaaaactc agcgtaaatt attccgtaaa
      781 taaatttttc acatctgcgt catggcgcgc tggtacgccc gcactgctga ccgaccccgc
      841 gcagtgtgcc cggcctgggc tgcacaacgc gatgcgtctt ctcaacccgt ttcaacccag
      901 ggaagggcga tacaggatga ccacacgtcg tgattttctg gcgggcgcca cagcggcggc
      961 tgcctttgcc agcatccgcc aggcgctggc cattccggca cggcgccgga ccggcaccat
     1021 caaggacgtc aagcacatcg taatcctgat gcaggagaat cgctcgttcg accactactt
     1081 cggcaccctg cgcggcgtac gtgggttcgg cgatcgcttt cccattccgc tcgaaagcgg
     1141 caagtcggtc tggttccagt ccgacggcac ccgtgagatc acgccctacc accgcgactc
     1201 cgcgaccagc aacgcgctgg tcgggtacgg cacgccgcat tccttcggcg acagtcaggc
     1261 ggcgtggaat cagggcaaga tgggcctctg ggccaagtac aagaccccgt acgcgatggg
     1321 ctatttcggt cgcgacgaca tcccgttcca gttcgcgctc gccgaggcat tcacactgtg
     1381 cgatgcctac cactgctcca tcaccaccgg caccgatccc aaccggatca cgttctggtc
     1441 gggctcgaac ttcaatcccg cgctgcgcgc gcagggcatc aactgcacca cgaacgattc
     1501 cgagcccaac aacagccgct gctggccgag cccgtcgaag tgggtggccg ggctgccgca
     1561 gccgcagccg acctacaaat atgtcaacag cgacttcgcc tgggacaccc ttcccgacct
     1621 gctgcagcgc gccggcgtca gctggcacat ctaccaggac atgaacgaca attggaccgg
     1681 cgcgatgcac ggctgtctgg cgttctccag cttccgccat gcacagccgg gtagccccaa
     1741 ctacgt
//
LOCUS       DATASH010000808         1512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6068642,
            whole genome shotgun sequence.
ACCESSION   DATASH010000808 DATASH010000000
VERSION     DATASH010000808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6068642"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="VHV99_21705"
     CDS             complement(<1..487)
                     /locus_tag="VHV99_21705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX3638047.1"
                     /translation="MRRRAWLAASVSAAAAMLASWAFDAHAGSPAARQVLVEAYGDST
                     TLGITCSDGHCGPQAQNAVSYLQDELQAAHGQRVRVTNYGVGGTMASQLRDGTGNRRA
                     GPTARLPWRERLAASSAQIVLINYGINEVMQNETPEQFYAAETTLVKTARALGKEPVL
                     QT"
     gene            complement(484..>1512)
                     /locus_tag="VHV99_21710"
     CDS             complement(484..>1512)
                     /locus_tag="VHV99_21710"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX3638048.1"
                     /translation="GLRAIAVLAVFAQHALKAPLWMGVDLFFVLSGFLITGILLERKA
                     RQQSYFGYFYARRARRILPPYVLLMVVSSILFGFGWAQHWQWYAFFAANIGDTLNQSG
                     HDSLNVLWSLAVEEQFYIFWPFVILLLPERVLGYVAAALIVLVPVLRALATPWFDSFW
                     PIYYLTPFRMDLLAAGALLAVAVRRDRHALEPFKGLAVLGFFAALTVLAWLHLHYPRF
                     RAANTPLSNAGLYSVSLVLCTSVVVIALQSKGIVKRLLCNPVLVYIGTISYTIYLIHL
                     SVLYALWPLHLNRYVTAALALAITVAYASITWFAFEKRLIFGSAGKAHASAGNVSGSA
                     SQAPQSRA"
BASE COUNT          311 a          481 c          487 g          233 t
ORIGIN      
        1 aggtctgtag aaccggctcc ttgccaagcg cccgggcggt tttcacgagc gttgtttccg
       61 ctgcgtaaaa ctgctcgggc gtctcgttct gcatcacttc gttaatgccg tagttgatca
      121 acacgatctg cgcggacgaa gcagcgaggc gttcgcgcca cggcaggcgg gcagtcggac
      181 cggcgcgccg gttgccggtg ccgtcgcgca actggctggc catcgtgccg cccacgccgt
      241 agttcgtcac gcgcacccgt tggccatgcg cggcctgcaa ttcgtcctgc agatacgaca
      301 ccgcgttttg cgcttggggt ccgcagtggc cgtcgctgca cgtgataccg agcgtggtcg
      361 aatcgccgta ggcttcgacc agcacctgcc tcgcggccgg gctgccggca tgcgcgtcga
      421 atgcccatga ggccagcatc gccgcagcgg ccgacacgct cgcggcgagc cacgcgcggc
      481 gcctcatgcg cggctttgcg gcgcttgcga cgcgcttcct gaaacattgc cggcgcttgc
      541 atgggctttg cctgccgagc cgaaaatcaa gcgcttttcg aacgcgaacc acgtgatgct
      601 cgcgtaggcg acggtgatcg cgagcgccag cgccgcggtc acgtagcggt tcagatgcag
      661 cggccacagc gcgtacagca cgctcaggtg gatcagataa atggtgtagc tgatggtgcc
      721 gatatagacc agcacggggt tgcacagcag ccgtttgacg atgcccttgc tttgcagggc
      781 gatgaccacg acggaagtac aaagcaccaa cgaaacgctg tagagcccgg cgttcgagag
      841 cggcgtattc gccgcgcgga accgcgggta atgcagatgc agccaggcaa gcacggtcag
      901 cgcggcaaaa aagccgagca cggcgaggcc cttgaatggc tcgagggcat ggcgatcgcg
      961 ccgcacggcg accgcgagta gcgcgccggc cgccagcagg tccatgcgga acggcgtcag
     1021 gtaatagatc ggccagaacg agtcgaacca cggcgtggcg agcgcgcgca gcaccggcac
     1081 cagaacgatc aacgcggccg ccacgtagcc gagcacgcgc tcgggcagca acaggatcac
     1141 gaacggccag aaaatataga actgctcttc aacggccagc gaccacagca cattcaggct
     1201 gtcgtgaccg ctctgattga gcgtatcgcc gatattggcc gcgaagaacg cgtaccactg
     1261 ccagtgctgc gcccagccga agccgaacag aatcgacgac accaccatca gcagcacata
     1321 cggcggcagg atgcgccgtg cccggcgtgc atagaaatag ccgaaatacg actgctggcg
     1381 tgccttgcgt tcgagcagga tgccggtaat cagaaagccg ctcaaaacaa aaaacagatc
     1441 gacgcccatc cacagcggcg ctttcagtgc gtgttgcgcg aatacggcaa gcacggcgat
     1501 ggcgcgcagg cc
//
LOCUS       DATASH010000809         4428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_7059173,
            whole genome shotgun sequence.
ACCESSION   DATASH010000809 DATASH010000000
VERSION     DATASH010000809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7059173"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            366..3545
                     /locus_tag="VHV99_21715"
     CDS             366..3545
                     /locus_tag="VHV99_21715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX3638049.1"
                     /translation="MKRNLFYRFLGRLRVGRKLLLIYLLDLSAVLYISGILIHEKYIA
                     IDFSDKELTGNAYVAAVHDALIHFALAGAGQPQTAAQLQRTASRLADAERRFGANMQS
                     AGINEQVLDALAASSAPHASAATVDRGLEATRALVTRVGNQSNLILDPDLDSYYSMSL
                     SILRYPELLGDVNRIGRHLHDAGASFSAARMSRDEMRTRYLVLEGQLDAVLQGLRSDY
                     AEASVANGTLTGTIGPPVGRLLAAIDAYRRVAGAAVDAGPDAVTLAALDAAQQRVVMS
                     VSDAWSVTGNQLERLLHERVRGLFERMWLHLGTALFLLCAILGMVYFVAQQISRPLRR
                     LARVMDTVRRTGDHSLRANWHSQDEIGQLVQGFNDMLAQLARERDVQKELEATARASA
                     AQYALVEATPVPMVVTAIPGHEVLHANGPALSWLNGCTVDPWAFGLDSEVRARFFQQL
                     SDRDAVDEFEVRWKAGSEPTWAMLSARRLDFQGRDAVLTAFTPINQIKLMEQRLELWG
                     KVFEASSEAILILDAQRRLVTANASFYRATGYRSDDVAGKAPAFLFGAQEQGDIFPAV
                     IDSVDRLSAWHGEAHVRRRAGSDYPAWLMVSAVRDRRANLSHYICTLIDITDRKKNEA
                     RIQFLAEHDVLTELPNRELFVKRLGVVLGNARRTGQRVAVLFIDLDRFKNINDSLGHH
                     IGDNLLRSVSRRLLQAVRSNDTVNRLGGDEFTVILNGVTNAADVSRTIEERLLPLVRQ
                     PHELGGVALPVSCSVGVALFPDDGEDIETLMQNADAAMYQAKGAGRNLVKFFAPDMAE
                     RARYRLTLEACLRTAIAMREFRLEYQPCVDAHTGELIAVEGLLRWNSSQIGSVSPAQF
                     IPVAEETQLIVPIGAWVIDQACQQVAAWDAEGLMQLRVSINLSAIQLRDPDLIGTLTD
                     SLARYRVAPERLELEITETVLMDNAENYLTTINAIRKLGVKLSLDDFGIGYSSLSYLN
                     RFPLDRLKIDRAFVQDMLDAPADLAIIRAIIELGHELGLRVVAEGVESEHQAHILRTI
                     GCDELQGFLFSKALSPADLWSWRTARMAA"
     gene            complement(3666..4070)
                     /locus_tag="VHV99_21720"
     CDS             complement(3666..4070)
                     /locus_tag="VHV99_21720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX3638050.1"
                     /translation="MFNFFDINSIIGQFPDKADSTIVDVYLTDEPSASSRIFRVYYPI
                     PRHYHETCEEHLYLLTGKVAFAIEDEAPRILSAGQLVTFERNTVHAITEILESPAIFF
                     TVDAPRRVPTDVVYVNPDDAKIRPFVTHLESY"
BASE COUNT          828 a         1355 c         1380 g          865 t
ORIGIN      
        1 aggagctgga ggcaggacgc gacgagctgc tgctcggcct catcgaactg cagcggaaat
       61 gtgcggattg accgctcgcc ggctaaggag acctcatggt gacaatgcct cattaccgtt
      121 acgcgaatga tccaacggag agccttattc gaaatgttcg caccgtgttg tcgcgtttcc
      181 ggcaccgtct tggagcgttt taagcaaaga ctaaagaaag tctcccactg gccgatagcg
      241 tgatcagggg cttttcccgc agtcggacct gacccaggtc cgacgttccc cgcacggaga
      301 aacgcagtga aacgtctggc ttgccgcacc cgggacgaaa gcttcgcgat cgagcgacgg
      361 cgcccatgaa acgcaacctg ttctaccggt ttctcggacg tcttcgcgtc ggccgcaagc
      421 tgctgctgat ctatctgctc gacctgagcg ccgtgctgta catcagcggc atcctgatcc
      481 acgagaagta catcgcaatt gacttctccg acaaggagct gaccggcaat gcctacgtcg
      541 cggccgtgca tgacgcgctt atccacttcg cactcgcggg cgccgggcag ccgcagactg
      601 cggcccaatt gcagcgcact gccagcagac tcgccgatgc cgagcgccgc tttggcgcga
      661 acatgcagag cgccgggatc aatgaacagg tgctcgatgc acttgcagcc agctcggcgc
      721 cgcatgcgag cgcagccacg gtcgaccgag gtcttgaagc gacgcgcgca ctggtgacgc
      781 gggtcggcaa ccagtcgaac ctgattctgg atccggatct cgacagctac tattcgatgt
      841 cgctgtcgat cctgcgctat ccggaactgc tcggcgacgt caaccggatc ggccgccacc
      901 tgcacgatgc cggcgcgagt tttagcgccg cacggatgag ccgcgacgag atgcgcaccc
      961 gctatctcgt gctggagggg cagctcgacg cagtattgca ggggctgcgc tccgactatg
     1021 cggaagcttc agtcgcgaac ggcacactga ccggcacgat cggaccgccg gtcgggcgat
     1081 tgctcgccgc gatcgacgcg tatcgccgtg tggctggcgc cgcagtcgac gccggcccgg
     1141 atgcggtcac gctggccgcg ctcgatgccg cacagcagcg cgtggtgatg agcgtaagcg
     1201 acgcgtggag tgtgaccggc aatcagctcg aaagacttct gcatgagcgc gtgcgcggcc
     1261 tcttcgagcg gatgtggctg cacctcggaa cagcactctt cctgctgtgc gcgatcctgg
     1321 gcatggtgta cttcgtcgcg cagcagatct cacggcccct gcgccggctc gcgcgcgtga
     1381 tggataccgt gcgccgcaca ggtgaccact ctctgcgcgc caactggcat agccaggacg
     1441 agatcggcca gctcgtgcaa ggcttcaacg acatgctcgc gcagctcgcc cgcgagcgcg
     1501 acgtgcagaa ggagcttgag gcgaccgcgc gcgcgtcggc cgcgcaatac gctctcgtcg
     1561 aggcgacccc agtgccgatg gtcgtcacgg cgatccccgg gcacgaggtg ctgcacgcga
     1621 acgggccggc gctttcctgg ctcaatggct gcacggtgga cccatgggcc ttcggtctcg
     1681 actcggaggt gcgcgcgcgt ttcttccagc aactctccga ccgcgatgct gtagacgaat
     1741 tcgaggtgcg ctggaaggcc ggcagcgaac ccacctgggc catgctgtcg gcgcggcgcc
     1801 tcgacttcca ggggcgcgac gccgtgttga ccgcattcac cccaatcaac cagatcaagc
     1861 tgatggagca gcggctcgag ctgtggggca aggtgttcga ggcgtcatcc gaggcgattc
     1921 tgattctgga tgcgcaacga cggctcgtga ctgccaacgc atcgttctat cgtgcgacag
     1981 gatatcgttc cgacgacgtg gcgggcaagg cgcccgcttt cctcttcgga gcgcaggagc
     2041 aaggcgacat ttttccggcc gttatcgatt ccgtcgaccg cctgagcgca tggcatggcg
     2101 aagcgcatgt gcggcggcgt gcgggcagcg actatccggc gtggctgatg gtgagcgccg
     2161 tgcgcgaccg gcgtgcgaac ctgtcgcact atatctgcac gttgatcgac attacggacc
     2221 gcaagaagaa cgaggcccgc atccagttcc tcgccgagca cgacgtgctg acggaactgc
     2281 cgaaccgcga gctgttcgtg aagcgccttg gcgtcgtact cggcaatgcg cgtcgcacgg
     2341 ggcagcgcgt cgcggtgctg ttcatcgatc tggaccgctt caagaacatc aacgattcgc
     2401 tcggccatca catcggcgac aacctgctgc gctcggtctc gcggcgcctc ctgcaggccg
     2461 tgcgcagcaa cgacacggtc aaccgcctcg gcggagacga gttcacggtg atcctcaacg
     2521 gcgtcaccaa cgcggcagac gtcagccgca cgatcgagga gcgtttactg ccgctcgtgc
     2581 gccagccgca cgagctcggc ggcgtcgcgc tgccggtgtc gtgtagtgtc ggcgtggcac
     2641 ttttccccga cgacggcgag gacatcgaga ccctgatgca gaacgctgac gccgcgatgt
     2701 accaggcgaa gggcgcggga cgtaacctcg tcaagttctt cgcgcccgac atggccgaac
     2761 gcgcgcgcta ccgcttgacg ctcgaagcgt gcctgcgcac cgcgatcgcg atgcgcgagt
     2821 tccggctcga atatcagccg tgtgtcgatg cgcacacggg tgaactgatc gctgttgaag
     2881 ggctgctgcg ctggaacagc tcgcagatcg gatcggtgtc gcccgcgcag tttattccgg
     2941 tcgctgaaga gacgcagctc atcgttccca ttggcgcctg ggtgatagac caagcctgcc
     3001 agcaggtcgc cgcgtgggac gcagaagggc tcatgcaatt gcgcgtctcg atcaacctgt
     3061 cggcaatcca gctgcgcgat ccagacctga tcggcacgct cacagacagt ctcgcacggt
     3121 atcgagtcgc ccctgagcgg ctcgaactcg agatcaccga gacagtgtta atggacaatg
     3181 cggagaacta tctcaccacg atcaacgcga tccgcaagct tggagtcaag ctttccctcg
     3241 acgatttcgg tatcggttac tcaagcctca gctatctgaa ccgattcccg cttgatcgcc
     3301 tcaagatcga tcgtgcgttc gtgcaagaca tgctcgatgc gccggccgac ctcgccatca
     3361 tccgggcaat catcgaactc ggccacgagc tgggtctacg ggtggtcgcc gagggcgtcg
     3421 agagcgagca tcaggctcac atcctgcgca ctatcggttg cgacgaattg cagggtttcc
     3481 tgttctcgaa ggccttgtcg cctgccgatt tgtggtcctg gcgtaccgcg cgcatggcgg
     3541 cgtgagcggc gcggtacgct tttcatggtc gtgtgaagca acccttcgcg tttctctaaa
     3601 gcggttgttc actggcagga aacggcaggt tgatagtctg atttcctgcc gccgcacgct
     3661 gacctttagt agctttccag gtgggtcaca aaaggccgga tttttgcgtc gtccggattc
     3721 acatacacga catcggtcgg cacacgacga ggagcgtcaa ctgtaaagaa gattgctggc
     3781 gactcgagta tttcagttat cgcgtggacc gtgttccgct caaaggtcac tagctggccc
     3841 gcagagagga tgcgaggcgc ttcgtcctca atcgcgaacg cgaccttacc ggtcaggagg
     3901 tagagatgtt cttcgcacgt ttcgtggtag tggcgaggta tcgggtaata aacacgaaag
     3961 attcttgagc ttgcagaagg ttcatcggtg agatagacgt cgacaatcgt gctatccgcc
     4021 ttgtccggga attgaccgat gatgctgttt atatcgaaaa aattaaacaa ttcgttctcc
     4081 gtgttatggg gtgaagcgac gaacaattcg acaggatgtg ccgttcggta gcgtcgacat
     4141 cgcgtggtgt atctggatga agccgtctat gcgctcatcg tggctgaatg catcctgcca
     4201 cgcggcagcg gaccggcgga atggcctcat ttggcaaatg acatttgcca aatgaggcta
     4261 tggttcatag ggaaccgttt cgctaccgcg taccattgaa ccccattgcg agcacgcccc
     4321 cgtcgctgtc gagctatctg cgtctgtgcc tggcctgctt acgattttct ttctttccac
     4381 aaccccgcgc ctgaggaggc gggggtcgtt gggggatttt tcgctcag
//
LOCUS       DATASH010000810         7023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5477582,
            whole genome shotgun sequence.
ACCESSION   DATASH010000810 DATASH010000000
VERSION     DATASH010000810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7023
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5477582"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VHV99_21725"
     CDS             complement(<1..518)
                     /locus_tag="VHV99_21725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HEX3638051.1"
                     /translation="MTIRVAINGYGRIGRNTLRAFYENGKKHDIEIVAINDLGDANTN
                     AHLTQYDTAHGKFPGEVSVDGDYLVVNGDKIRVLANRNPAELPWGELNVDVVMECTGF
                     FTTKEKASAHIKGGAKKVIISAPGGKDVDATIVYGVNHNVLKASDTVISNASCTTNCL
                     APLVKPLNDKIGL"
     gene            complement(614..2635)
                     /gene="tkt"
                     /locus_tag="VHV99_21730"
     CDS             complement(614..2635)
                     /gene="tkt"
                     /locus_tag="VHV99_21730"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX3638052.1"
                     /translation="MTTPSPSPTSLMANAIRALSMDAVQKANSGHPGMPMGMAEIGVA
                     LWSRHLRHNPKNPQWADRDRFILSNGHGSMLLYSLLHLTGYDLPIEELKNFRQMHSKT
                     PGHPEYGITPGVETTTGPLGQGLANAVGMALAESLLATEFNKPDAKIVDHHTYVFVGD
                     GCLMEGISHEACSLAGVLKLNKLIAFYDDNGISIDGEVVHWFHDDTPKRFEAYGWNVI
                     PNVVGHDVDAVDAAIKQAKQSDKPTLICCKTVIGEGAPTKAGSHDSHGSPLGDKEIAA
                     TREKIGWKWEPFVIPQEVYAAWDAKEAGARNESDWDKAFAAYAAKYPQEAAEFKRRDA
                     KQLPADWKEKAKAIIAGANERAETVATRKASQQAIEGLSAVLPELLGGSADLTGSNLT
                     NWKAARPVRVNAEGKAAGNYVNYGVREFGMSAAINGIALHGGFKAFGGTFLTFSDYSR
                     NALRVAALMKAPSIFVFTHDSIGLGEDGPTHQSIEHVASLRLIPNLQVWRPADTVETA
                     VAWTHAVEHHGPSCLIFSRQNLPFSERTDAQIANIEKGGYVLRDWNDEIVARKVILIA
                     TGSEVELALNAVEPLAREGIAARVVSMPSTTVFDKQDAEYRERVLPHGVRRVAIEAGV
                     TDFWRKYVGLEGGVVGIDTFGESAPAGVLFKHFGFTVEHVVETAKAALG"
     gene            2954..3835
                     /gene="speE"
                     /locus_tag="VHV99_21735"
     CDS             2954..3835
                     /gene="speE"
                     /locus_tag="VHV99_21735"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEX3638053.1"
                     /translation="MSASLLFHPAHDAVYGFEHARRLASVSSPYQHIEVWDTPQLGRL
                     FTLDGRPMTAVGDEFIYHECMVHPAALAHPAPRTALVLGGGDGGAARQLLKHPSIERI
                     VVAELDAEVVRMTREYLPEVHGGAFDDPRVELAIGDAAHFVSGAAASATAQFDLVVFD
                     LTPPDSPAAGLYTPDFYIQLKRVMRPTAALSLHLGSPYFQPQRVAGLLDDLRDTFAFV
                     RTMHTFVPLYGSLWMMATASDTLDPAALGVEALAERIAARQIDSLKHYDPALHAGLFS
                     ASRSVRDKLSQFLKPSS"
     gene            3950..4363
                     /locus_tag="VHV99_21740"
     CDS             3950..4363
                     /locus_tag="VHV99_21740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3638054.1"
                     /translation="MTASRPAGVPWLTPYLTVRDARAATAFFEAAFGFEVRDSVQDDG
                     VVMHVEMTYQGQLIVMFAPEGAFGSAAKTPKSAGAIAPQSFYVYVDDVDAVYTRALAA
                     GAKSLSEPQDQFWGDRFAQVEDLDGYRWALARHLS"
     gene            4383..5123
                     /locus_tag="VHV99_21745"
     CDS             4383..5123
                     /locus_tag="VHV99_21745"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEX3638055.1"
                     /translation="MPRFFVGTPLKPDDIMQLPDDVMRHILVLRLQPGDSIVLFNGEG
                     GEYSAELVEVERRSAKVRIHEFRNIEVEAPYHLTLAQGIAGGDKMDWLIEKAVELGAS
                     SFVPLTTTRSVVRLSGDRAQRRHVHWQGIVRASCEQCGRNRLPEVMPVREIATWLGAL
                     PRTPEVGEMRILLSPRASISFSALPANPPFGRVTVLVGPEGGFSAAEEAAATDHGFTA
                     VGLGPRVLRTETAGIAVLSALAARWGGW"
     gene            complement(5425..5985)
                     /locus_tag="VHV99_21750"
     CDS             complement(5425..5985)
                     /locus_tag="VHV99_21750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HEX3638056.1"
                     /translation="MSDNVYAHDSGVATDLFAAGDGNLFQRVMRMRAGDQGRDNQSEG
                     STVVSSNEEPMSLFKTVRPNIVQSIRAFRVQDLADEANQLGQHFLYAYCANAQSKQEV
                     LETIATSFLLPKHFGKNYDALYDCLTDLVQKAGAQPGFVIVLEQLPVAQKFDKEGRET
                     LLDVFREAAEFWAERKVAFRVFYSFA"
     gene            complement(6002..6418)
                     /locus_tag="VHV99_21755"
     CDS             complement(6002..6418)
                     /locus_tag="VHV99_21755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease"
                     /protein_id="HEX3638057.1"
                     /translation="MARKWQRMKGVLTGALTLCALCGSVPGAFARDYTAPAESQAQVQ
                     GTIAAAQLPREAVNTLNLIAAGGPYAYEKDGIVFGNRERLLPPHRRGYYHEYTVPTPR
                     SRNRGARRIVCGGPLRRIDNCYYSDDHYTSFNRIVE"
     gene            complement(6834..>7023)
                     /locus_tag="VHV99_21760"
     CDS             complement(6834..>7023)
                     /locus_tag="VHV99_21760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HEX3638058.1"
                     /translation="LPNIDAANISYNLLKTAAGNNIAIGPMLLGAARPVHVLTASATV
                     RRIVNMTALLVADVIASR"
BASE COUNT         1260 a         2216 c         2145 g         1402 t
ORIGIN      
        1 aggccaatct tgtcgttcag cggcttgacc agcggtgcca ggcagttggt cgtgcacgat
       61 gcattcgaaa tcaccgtgtc cgatgccttg agcacattgt ggttcacgcc gtagacgatg
      121 gttgcgtcga cgtccttacc gcccggcgcc gagatgatga ccttctttgc gccacccttg
      181 atgtgcgcgc tagccttttc cttggtcgtg aaaaagcccg tgcattccat caccacgtcc
      241 acgttcagct cgccccacgg cagttctgcc gggttgcggt tagccagcac gcggatcttg
      301 tcgccgttga cgacgaggta gtcgccgtcc accgacactt cgcccgggaa cttgccgtgc
      361 gccgtatcgt attgcgtcag gtgagcgttg gtgttggcat cgccaagatc gttgatggcg
      421 acgatttcga tatcgtgctt cttgccgttt tcatagaagg cgcgcagcgt gttgcggccg
      481 atccggccgt agccgttgat tgcgacgcga atcgtcatgg tctatctcct gatggctgaa
      541 aaaaatcgtc catgttggtc tgatttgccg ggcttgccgc gcgtggggta cgcgcggcag
      601 cctcaaacgc agatcagccg agtgcggctt ttgccgtctc gacaacgtgc tcgacggtga
      661 agccgaaatg cttgaacagc acgccagccg gggccgattc gccgaacgtg tcgatgccga
      721 ccacgccgcc ttccagaccc acgtacttgc gccagaaatc cgtgacaccc gcttcgatcg
      781 caacacggcg cacgccgtgc ggcagcacgc gttcgcggta ttcggcgtcc tgcttgtcga
      841 acacggtggt cgacggcatc gacacgacac gtgccgcgat gccttcgcga gccagcggct
      901 cgaccgcgtt caacgccagt tcgacttccg aaccggtggc gatcaggatg accttacgcg
      961 cgacgatctc gtcattccag tcgcgcagca cgtagccgcc cttctcgatg ttggcgatct
     1021 gcgcgtctgt gcgctccgag aacggcaggt tctgacggct gaagatcagg cacgacgggc
     1081 cgtgatgttc gaccgcgtgg gtccaggcca ccgccgtttc gaccgtatcc gccgggcgcc
     1141 acacttgcag attcgggatc agacgcaggc tcgcgacgtg ttcgatcgac tggtgggtcg
     1201 ggccgtcttc accgaggccg atcgagtcgt gcgtgaacac gaagatcgac ggtgctttca
     1261 tcagcgcagc cacgcgcaat gcattgcggc tgtagtccga gaacgtcagg aacgtgccgc
     1321 cgaaggcctt gaagccgccg tgcagcgcga tgccgttgat cgcggcgctc atgccgaact
     1381 cgcgcacacc gtagttcacg tagttgccgg cggccttgcc ttcagcgttc acacgcaccg
     1441 gcctggcggc cttccagttg gtcagattcg aaccggtcag atcggcggaa ccgccgagca
     1501 gttccggcag cacggccgac aaaccttcga tagcttgttg cgatgccttg cgcgtcgcga
     1561 cggtttccgc gcgttcgttc gcgccggcga tgatggcctt cgccttttcc ttccagtcgg
     1621 cgggcaattg cttcgcgtcg cggcgcttga attcagcggc ttcctgcggg tatttggccg
     1681 cgtacgctgc aaacgccttg tcccagtcgg attcgttacg tgcgccggct tccttcgcgt
     1741 cccacgctgc atagacttcc tgcggaatca cgaacggctc ccacttccag ccgatctttt
     1801 cacgcgtcgc cgcgatttcc ttgtcgccga ggggcgagcc gtgcgaatcg tggctgccgg
     1861 ccttggtcgg cgcgccttca ccgatcacgg tcttgcagca gatcagcgtg ggtttgtccg
     1921 attgcttggc ttgcttgatc gcggcgtcga ccgcgtcgac gtcgtggccg accacgttcg
     1981 ggatcacgtt ccagccgtac gcttcgaagc gcttcggggt gtcgtcgtgg aaccagtgga
     2041 ccacttcgcc gtcgatcgag atgccgttgt cgtcgtagaa cgcgatcagc ttgttcagct
     2101 tcagcacgcc cgcgagcgag caggcttcgt gcgagatgcc ttccatcagg cagccgtcgc
     2161 cgacgaacac atacgtgtga tggtcgacga tcttcgcgtc aggtttgttg aattcggtgg
     2221 cgagcagcga ttcggcgagc gccatgccga ctgcattcgc gaggccttgg ccgagcgggc
     2281 ccgtggtggt ttcgacgccc ggcgtgatgc cgtattccgg gtgacccggc gtcttggaat
     2341 gcatctggcg gaagttcttc agctcttcga tcggcaggtc gtagccggtc aggtgcagca
     2401 gcgagtacag cagcatcgag ccatggccgt tcgacagaat gaaacggtcg cgatcggccc
     2461 actgcgggtt cttcggattg tggcgcagat gacgcgacca caaagccacg ccgatttcgg
     2521 ccatgcccat cggcatgccc gggtgaccgg aattcgcttt ttgaacggca tccatggaca
     2581 acgcgcggat tgcgttggcc atcagggaag tgggtgaggg agacggggtc gtcatgtcga
     2641 gtccggagac agagtcagaa agcggtgcgc gcttgaggac cggatgctcg aatgccagtc
     2701 cactgcggcg cacggtgcgg cgccacgaga cgcgaaacgg gcagagcaaa cggacaaggc
     2761 tgaaagcgtg acattctaac agaacgttga tcgcggcccg ctcgggaagc cgcctggtgc
     2821 agtccggtcg tgccggctga gaaggcagct cgacgcgacg gctgcggcgc gcggagcgtc
     2881 tgccctttac aatcgggcaa cctcgattca ctctctcaat gtgccactgt gctccgcacg
     2941 gagcccgccc ttcatgagcg cttccctgtt gttccaccca gctcacgacg ccgtctacgg
     3001 ctttgagcac gcgcgccggc ttgcgagcgt gagctcgccg tatcagcaca tcgaggtgtg
     3061 ggacacgccg caactcggcc ggctattcac gctcgacggc cggccgatga ccgccgtcgg
     3121 cgacgaattc atttaccacg aatgcatggt gcatccggcc gcgctggcgc atccagcgcc
     3181 gcggacagcg ctggtgctgg gcggcggaga tggtggcgcc gcgcggcaat tgctcaagca
     3241 ccccagcatc gagcggatcg tggtggcgga gctcgatgcc gaagtcgtac gcatgacccg
     3301 cgagtatctg cccgaggtcc acggcggcgc tttcgacgat ccgcgcgtcg aactggcgat
     3361 cggcgatgcc gcgcacttcg tctcgggcgc tgcggcctcc gccaccgcgc agttcgatct
     3421 cgtcgtcttc gacctgacgc cgccggattc ccccgctgcg ggcctatata caccggactt
     3481 ctacatccag ctcaagcgcg tcatgagacc gaccgccgcg ctctcgttgc atctcggttc
     3541 tccctatttc cagccgcagc gcgtcgctgg cctgctcgac gatctacgcg acacattcgc
     3601 cttcgtgcgc acgatgcaca ccttcgttcc gctctacggt tcgctctgga tgatggcgac
     3661 cgccagcgac accctcgacc ctgcagccct cggcgtcgaa gcgctcgccg agcgtattgc
     3721 cgcgcgccag atcgactcct tgaagcacta cgacccggcc ctgcatgcgg gactgttttc
     3781 ggcatcccgc tcagtgcgcg ataaactaag tcaattctta aagccatcaa gctaacgcgt
     3841 gggcaaaatg cacggttcgg tcggcgtgcg ctatcggcgt atcgatacgc aaccggtcgc
     3901 ctggcctcgc agcatgcatc ccgcaacctc aatagatccg gagaacccca tgaccgcctc
     3961 ccgcccagcc ggtgtgccct ggttgacccc ctatctgacg gtgcgagacg cacgcgccgc
     4021 gaccgcgttc ttcgaagcgg cattcggttt cgaggtgcgt gacagcgtcc aggacgatgg
     4081 tgtcgtcatg cacgtggaga tgacttatca aggccagctg atcgtgatgt tcgcacctga
     4141 aggcgcgttc ggctcggcgg cgaaaacacc gaaaagcgcg ggcgcgattg cgccgcaatc
     4201 gttctatgtc tatgtcgacg atgtcgacgc ggtttacacg cgggcgctgg ccgccggcgc
     4261 aaaatcgttg agcgagccac aggatcagtt ctggggcgac cggtttgccc aggtcgaaga
     4321 tctggacggc taccgttggg cgctcgctcg ccacctttcc tgaaccaatg agtatttttc
     4381 tgatgcctcg cttcttcgtc ggtacgcctc tcaagcccga cgacatcatg cagttgcccg
     4441 atgacgtcat gcgccacatc ctcgtgctgc gtttgcagcc cggcgattcg atcgtgcttt
     4501 tcaacggcga aggcggcgaa tacagcgccg aactcgtcga ggtcgaacgc cgctcggcca
     4561 aggtgagaat tcacgaattc cgcaatatcg aggtagaagc gccgtatcac ctcaccctcg
     4621 cgcagggtat tgccggcggg gacaagatgg actggctcat cgagaaggcg gtcgaacttg
     4681 gagcctcgtc gttcgtgccg ttgaccacca cgcgcagtgt cgtgcgtctt tccggcgacc
     4741 gcgcgcagcg gcgtcacgtg cactggcagg gcatcgtgcg ggcgtcgtgc gaacagtgcg
     4801 ggcgcaatcg cctgccggaa gtgatgccgg tgcgcgagat cgccacgtgg ctcggcgcgc
     4861 tgccgcgcac gcctgaagtc ggggaaatgc gtattctcct gtcgccgcgc gccagcatca
     4921 gcttttcggc gctgcccgcc aatccgccgt tcgggcgagt gaccgtactg gtcggcccgg
     4981 aaggcggttt ttccgctgcg gaagaagccg ctgcaaccga tcatggattt actgcggtcg
     5041 gtctcgggcc gcgcgtgttg cgcacggaaa ctgcgggcat cgccgtgctc tcggcattgg
     5101 cggcccgctg gggcggctgg taagccagac tacgcgccag gcacagccag cttgggtggg
     5161 ataagcgaga cggcaggcga gagccaagct ggtcagctgt tcggccttag agctaagcca
     5221 agctaagcca ggtcaggtca ggtcaggtca ggtcaggtca ggtcaagctt accaaaccgg
     5281 cccaagctaa atgaagccgt ggcttcaaaa ccatacgctg gttcaaaacc ccaaaacaaa
     5341 ggcccgccag ttagcgggcc tttctctttc cttcgcggta gccgacgcgg agataaatcg
     5401 cccgcgcaaa ccacccgaca aatttcaggc aaacgaataa aacacccgga acgcgacctt
     5461 gcgttcggcc cagaactcgg ccgcttcacg gaacacgtcg agaagcgtct cacgcccttc
     5521 cttgtcgaac ttttgcgcaa ccggcagttg ttcgagcacg atcacgaagc cgggctgcgc
     5581 gccggccttc tggaccagat cggtcaggca atcgtagagc gcgtcgtaat tcttgccgaa
     5641 atgcttcggt aacaggaacg acgtcgcaat ggtctccagc acttcctgct tggactgagc
     5701 gttcgcacag tacgcgtata gaaagtgctg gccgagttga ttggcctcgt cagctagatc
     5761 ctgcacgcga aacgcgcgga tcgactgtac gatgttaggt cgtacggtct tgaaaaggct
     5821 catgggctcc tcgttcgatg aaaccacagt gctgccttcg ctctggttat ccctgccctg
     5881 gtcgccggca cgcatccgca tgacgcgctg gaacaaattg ccgtcgccgg ccgcgaaaag
     5941 atccgtcgcg actccggagt cgtgcgcgta gacgttgtcg ctcatgccgt tcatcccgat
     6001 gtcattcaac aatacgattg aaactggtgt aatggtcgtc cgagtagtaa cagttgtcga
     6061 tccgccgtag cggacctccg cagacgatgc gacgcgcccc acgatttcgt gaacgaggcg
     6121 tgggaacggt gtattcgtgg taatagccgc gccgatgcgg cggtagcaac cgttcgcgat
     6181 tgccgaatac gatgccatcc ttctcatacg cgtaaggccc gcccgcggca atcaagttca
     6241 atgtattgac cgcttcgcgt ggcaactgcg ccgccgcgat agtgccctgc acctgtgctt
     6301 gtgactcggc gggtgccgtg tagtcgcgcg caaacgcgcc aggcacggag ccgcataaag
     6361 cgcagagcgt caaagcaccg gtcagcacgc ctttcatgcg ctgccacttg cgtgccatga
     6421 atcaaggttc cccgctgtta gatcacgagt ttgacgacca tgaaagtcgt aagggtatcg
     6481 ctaaaacccc acagaatcaa cgttcaccgg acacgcgaaa gcgcccaatc gcgcgggaaa
     6541 gagccacgga tcgggctttg acgacttttg acagattatt tagcctgtac aggctaaaat
     6601 tagcccgatg aagcggaccg gtgcgccaag ccttcacgct atccagtgcg cagccatttt
     6661 ctgaagtgaa agccttgcgg tttctacagt ctttcggggg cggaatggcc tcgttcagcc
     6721 ccttttaggg tggcaatccc ctgttcaccc gcaggcgtgc ccattgcggg cacgcctttt
     6781 tttgcatggc gcccgccaaa ggccacgcca cgctgttagt cccgcatcag gaatcagcgg
     6841 gacgcgatca catccgccac cagcagcgcc gtcatgttga cgatccggcg caccgtggcc
     6901 gacgccgtca gcacatgcac cggcctggcc gcgcccagca gcatcgggcc aatcgcgatg
     6961 ttgttgcccg ccgcggtctt cagcaggttg tacgagatgt tggccgcgtc gatgttcggc
     7021 agc
//
LOCUS       DATASH010000811         3829 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5477736,
            whole genome shotgun sequence.
ACCESSION   DATASH010000811 DATASH010000000
VERSION     DATASH010000811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5477736"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(232..954)
                     /gene="mtgA"
                     /locus_tag="VHV99_21765"
     CDS             complement(232..954)
                     /gene="mtgA"
                     /locus_tag="VHV99_21765"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEX3638059.1"
                     /translation="MTATRRGSRPGPVRWVFYLGAVVAIAWFATQAFYFAQIAIWNYV
                     NPQSTAFMRSDAWRLSQDRPDLSVQHTWVPYEQISHNLKRAIIASEDANFVNNNGYET
                     DAILQAWERNKAKGKIVRGGSTITQQLARNLFLSREKSYIRKGQELIITWMLETLMDK
                     QRIFEIYLNSVEWGNGVYGAEAAAHYYYKTSAAKLTAAQSARLAVMLPQPKYFDEHRG
                     SGYLAQRSRVIARRMGAAELPE"
     gene            complement(1020..1883)
                     /gene="aroE"
                     /locus_tag="VHV99_21770"
     CDS             complement(1020..1883)
                     /gene="aroE"
                     /locus_tag="VHV99_21770"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX3638060.1"
                     /translation="MSANEARDRYAVIGNPVGHSKSPFIHGRFAAQTGEPIEYGHLLA
                     PVDAFVPHVRAFIEAGGRGLNVTVPFKLDAHAFADTMSPRAAAAGAVNTLRFDASGVY
                     GDNTDGFGLVRDIEVNLGVPLKGARILLLGAGGAARGVVLPMLDRAPHTLTIVNRTAA
                     KAEALVEQFADAARGAACRLSGGSARAIEAGTYDVIVNATAGSLDASLPECDDRAFGG
                     GTLAYDMMYGGHPTVFMEHAQKLGARGADGLGMLVEQAAESFYVWRGVRPDGAPVLAE
                     LRALLTAPPHA"
     gene            complement(1880..>3829)
                     /locus_tag="VHV99_21775"
     CDS             complement(1880..>3829)
                     /locus_tag="VHV99_21775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEX3638061.1"
                     /translation="DAAAQDIDLDFLWECAPEDEFPFATLGAEYFGESFGSIERAALV
                     LRMHGAPVYFRRKGRGMYQRAPQEQLKLALAGLERKRQQALVQAGYEDELKAGRLPEG
                     FSGSKALALLTRPDKNTIEYKALEGAAAARGISQARLMLECGGIPSARALHEAKFLSE
                     FFPHGTGFPPVTVGTLPEDLPEADVQAFSIDDVTTTEIDDAFSVEHLADGRVRIGVHI
                     AAPALGIVRGDEVDEIARARLSTVYMPGDKITMLPDSVVEAFTLAEGGLRPALSLYVI
                     INRETQEIVASETRAERVFVKNNLRHNTLDELVTEEALAAGTGDYPHKEDIAVLWPFA
                     QALFEKRQTARAGYGLRREVQRNTDFNFYVEGEHVTITPRRRGSPLDTIVAELAILAN
                     SSWGAFLHDHGVPGIYRTQRAFGAPTGPKRTRMQTNAAPHEGLGVTQYAWSTSPLRRY
                     VDLVNQWQLLACVQHGVTAKLAAPFKPKDADLFAVVQGFDDTYTAYADHQRRMEYFWC
                     LRWLKQENRKQVVASVVKGDLVRLEEIPLLLHVPGLGVHARGTRLQMEVMSLDELTVE
                     ASVRLLHVLDAPTVTSGSEAEEADEGEEEIIDVADESAEGEAEAQAEADLDGGAAEPG
                     DAENGAGADVGDASADQQHVAEPGR"
BASE COUNT          719 a         1320 c         1116 g          674 t
ORIGIN      
        1 ccgcttaaaa attagcggtg tgagccgctt tctcttgctt acttctcttt gcggcggcaa
       61 agagaagtaa gtgcccgccc cgcacagggg cgacgctaat agaccaataa caaatcaagg
      121 aaaggccaac gccgcaggca aacagaaaca agcgccgcgc aggcaacccg atcacgaaaa
      181 ggcccacccc gcacgcataa aaacaaaaaa ccagccgggc aggcccaacc cttattccgg
      241 caattccgcc gcccccatcc tcctcgcaat cacgcgcgaa cgttgagcca gataccccga
      301 gccccggtgc tcatcaaaat acttaggctg cggcagcatc accgccaacc gggccgactg
      361 cgcagcagtc aattttgccg ccgaagtctt gtagtaataa tgcgcggccg cctccgcgcc
      421 ataaacccca ttcccccact caacggagtt gagataaatc tcaaagatcc gctgcttatc
      481 catcaaggtt tcgagcatcc acgtaatgat cagctcctgc cccttgcgga tataactctt
      541 ctcccgcgac aaaaacagat tccgcgccag ctgttgggta atcgtcgacc cgccccgcac
      601 aatcttgccc ttggccttat tacgctccca cgcctgcaaa atggcgtccg tttcgtagcc
      661 gttgttattc acgaaattcg cgtcttcgga agcgataatc gcacgcttca ggttgtgcga
      721 aatctgctcg tacggcaccc acgtatgctg caccgaaaga tcaggccgat cctgcgacaa
      781 ccgccacgca tccgatcgca tgaaagccgt cgactgggga ttcacataat tccaaatcgc
      841 gatctgcgcg aagtaaaacg cctgcgtcgc gaaccacgca atcgccacca cggcccccag
      901 ataaaacacc caccgcaccg gacctggccg gctcccgcgc cgcgttgctg tcatcgtgac
      961 tggagtcctc aagtcggtga atcggtgagt gagcgtgttc cggaaaataa tgcagcgatc
     1021 taggcgtgcg gcggcgccgt caacagcgct cgcaattcgg ccagcaccgg tgcgccgtca
     1081 ggacgcacgc cacgccacac gtagaacgat tcggctgcct gctcgaccag catgccgaga
     1141 ccgtcggcgc cacgcgcacc gagcttctgc gcgtgctcca tgaacaccgt cggatggccg
     1201 ccgtacatca tgtcgtaagc gagcgtgccg ccgccgaacg cacggtcgtc gcactcgggc
     1261 aacgaagcat ccagactgcc cgccgtcgcg ttgacgatca cgtcgtacgt gcccgcctcg
     1321 atcgctcgcg cactgccgcc gctcagacgg caagcggcgc cacgtgcagc gtcggcgaac
     1381 tgctcgacca gcgcttcggc cttcgccgcg gtgcggttga cgatcgtcag tgtatgcggc
     1441 gcgcgatcca gcatcggcag cacgacgccg cgcgcggcac cgcctgcgcc aagcagcaaa
     1501 atccgcgcgc ccttcaacgg cacaccgaga ttcacctcga tgtctcgcac gaggccaaag
     1561 ccgtctgtgt tgtcaccgta gacaccgctc gcgtcgaacc gcagtgtgtt caccgcaccc
     1621 gcggccgccg cacgcggcga catggtgtca gcgaacgcgt gtgcgtcgag cttgaacggc
     1681 accgtgacgt tcaagccgcg tccgcccgct tcgataaaag cccgcacatg cggcacgaaa
     1741 gcatcgaccg gcgcgagcaa gtgcccgtac tcgatcggct cgccggtctg cgcggcaaac
     1801 cggccatgaa tgaacggcga cttgctatga ccgaccgggt tgccgatcac cgcgtagcga
     1861 tcgcgtgctt cgttagcgct catcgtcctg gctccgccac atgctgctga tccgctgatg
     1921 cgtcgccaac gtcggcgccg gcgccgtttt cggcgtcgcc cggttcagcc gcgccgccgt
     1981 cgaggtccgc ttcggcttgc gcctcggctt cgccttcggc gctttcgtcc gccacgtcga
     2041 tgatttcctc ttcgccctcg tcggcttcct cagcttcgct accgctcgtc acggtcggcg
     2101 cgtcgagcac gtgcaacagg cgcacggacg cttcgacggt cagttcgtcg agcgacatca
     2161 cttccatctg caagcgcgtg ccacgcgcgt gcacgccgag acccggcacg tgcagcagga
     2221 gcggaatctc ctcgaggcgc acgagatcgc ccttcaccac cgacgccacc acctgcttcc
     2281 ggttctcctg cttcaaccac ctcaggcacc agaaatactc catgcggcgc tggtgatcgg
     2341 catacgcggt gtaggtgtcg tcgaaacctt gcacgacggc gaacagatcg gcgtccttcg
     2401 gcttgaaggg tgcggccagt ttcgcggtca cgccgtgctg cacacaggcg agcaattgcc
     2461 actggttcac aaggtcaaca tagcggcgca gcggtgacgt gctccacgcg tattgcgtga
     2521 cgcccagccc ctcgtgcggc gcggcgttgg tttgcatgcg ggtgcgcttg ggaccagtgg
     2581 gcgcgccgaa cgcgcgctgc gtacgataaa tgcccggcac accgtggtcg tgcaggaacg
     2641 caccccacga cgagttggcg agaatcgcga gctccgcgac gatcgtgtcg agcggcgacc
     2701 cgcgccggcg cggcgtgatg gtgacgtgct cgccttccac gtagaagttg aaatcggtgt
     2761 tgcgctgcac ttcgcggcgc aggccgtaac cggcgcgcgc ggtctgacgc ttttcgaaca
     2821 acgcctgcgc gaagggccac agcacggcga tgtcttcctt gtgcggatag tcgccggtgc
     2881 cggcggcgag cgcttcttcg gtgaccagct cgtccagcgt gttgtggcgc agattgttct
     2941 tcacgaacac gcgctcggcg cgggtttcgc ttgccacgat ttcctgcgtc tcgcgattga
     3001 tgatcacata cagcgacaac gccggccgca agccgccttc ggccagcgtg aacgcttcga
     3061 cgacgctatc gggcagcatt gtgatcttgt cgccaggcat atagacggtc gacaagcgcg
     3121 cgcgtgcaat ctcgtcgacc tcgtcgccac gcacaatgcc gagcgcgggc gcggcgatgt
     3181 gcacgccgat ccgcacccgg ccgtcggcca gatgctcgac ggagaatgca tcgtcgattt
     3241 cagtggtggt gacgtcgtcg atcgagaacg cctgcacgtc ggcttccggc agatcttccg
     3301 gcaacgtgcc gacggtgacc ggcggaaaac cggtgccgtg cgggaaaaac tcggagagga
     3361 atttcgcctc gtgcagcgcg cgcgccgacg gaatcccgcc gcattcgagc atcaggcgcg
     3421 cctgcgagat gccgcgcgcc gctgcggcgc cctcgagtgc cttgtattca atcgtgttct
     3481 tgtcgggccg ggtgagcaaa gccagcgcct tgctgccgct aaagccttcc ggcaaacggc
     3541 ctgccttcaa ttcgtcttcg taacctgcct gcaccagcgc ctgctgacgc ttacgctcca
     3601 gcccggcaag cgccagcttg agctgctcct gcggcgcgcg ctggtacatg ccgcggccct
     3661 tgcggcgaaa gtagaccggc gcgccgtgca tgcgcagcac cagcgccgcg cgctcgatcg
     3721 agccgaacga ttcgccgaaa tactcggcgc ccagcgtggc aaacgggaat tcatcttccg
     3781 gcgcgcattc ccacagaaaa tccagatcga tgtcctgcgc ggcggcgtc
//
LOCUS       DATASH010000812        11238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5478062,
            whole genome shotgun sequence.
ACCESSION   DATASH010000812 DATASH010000000
VERSION     DATASH010000812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 11238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11238
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5478062"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(199..2352)
                     /locus_tag="VHV99_21780"
     CDS             complement(199..2352)
                     /locus_tag="VHV99_21780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004629 - phospholipase C activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase C, phosphocholine-specific"
                     /protein_id="HEX3638062.1"
                     /translation="MTSTSRRRFLQTVASSAGAAAALTALPESIRNALAVPAFSRTGT
                     IRDVEHIVVFMQENRSFDHYFGHIRGVRGYNDRFPIPLPGGKPVWYQPSKEDPTQPVL
                     PFHLNTATTSAQCLGDLDHNWYPTHAAINNGRYDQWPANKTDMTMGYHLRSDLPFHYA
                     LADAFTICDAYFCSLAGPTHPNRAYLMSGMVDPTGTQGGPLLDNNDFVDGDGPPKYQL
                     LSWTTYPERLQAAGISWQVYQQGLTGADPLNGNYGTNILQNFTNFVDAQPGSPLYERA
                     QTVRTIDDLKADVLANKLPQVSWLCPPAAYSEHPSYTPAYGAEYTSQILDALTSNPEV
                     WSKTVLFIMYDENDGFFDHLVPPQPATTPAQGQSTVSTDGEIHNVVNPGRGGSYTADG
                     LPYGLGPRVPMTIVSPWTKGGFVCSQVFDHTSVIRFIETRFGVQEPNITQWRRAVCGD
                     LTTAFDFRTPDSKVPPLPDTSNYKSIADNQCATQPKPTVPATPGVIDPQESGIRFARA
                     LPYELHVNGQTDVKKNTFEIVIANTGDQGAHFYVYATNRTDGPWRYTVEAGKSLSETF
                     DLTTTNGVYAFEVFGPNGFVRKFAGNTQQATAQTAQAAGWHGNNKPALPEIKVQYDVA
                     NGNVFLKFSNGGGGIARLTVTDNAYGARTRPVLVPAGANIEEAWVLASSHHWYDLTVT
                     SNDDASFSRRLAGHVENGRPSISDPAAVAPVLVVN"
     gene            complement(2413..2856)
                     /locus_tag="VHV99_21785"
     CDS             complement(2413..2856)
                     /locus_tag="VHV99_21785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638063.1"
                     /translation="MIHSQTFHNYFLKGLETASAITKLDADMRRHALLAITVFVAIAT
                     SLLSGCGADDAAPSSSATTAANSDAAQSAAMTPLSPGIANNDPAQSSAINPATDMFNA
                     PASLAAGPAAASDPIMQNIQASLAADSQQIAPVMHYAPGDNASSN"
     gene            2986..5145
                     /locus_tag="VHV99_21790"
     CDS             2986..5145
                     /locus_tag="VHV99_21790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEX3638064.1"
                     /translation="MTRIERPFDITRLEDEWLEADGFGGFASGTVGTLRTRRYHALLL
                     TATRAPGGRVVLVNGVEAWIEADGQRYPLSMQRYMPDVIYPDLTASLLAFDTDPWPTW
                     RLQLDPHTTLIAEVFVSKATRETVLRWRLQSDEADAPAKAFNLKVRPLLSGRDYHALH
                     HENPAFNFNAQTSGDAACVNWQPYGDLPIINAVTNGTYAHAPDWYRNFCYVRERERGL
                     DFSEDLATPGVFSFNLADGEAVMILSASNASPASATSATSATSATEVAGAQAVAAYAA
                     ESVGAAAFSTSQAGAKPAAYQTNSASTRPAAADTADSATTSPAAAHATQLAHTEQQRR
                     SALGSRLERSADAYVVARSNGRTVLAGFPWFTDWGRDTFIAMRGLLIATGRLAEAEAI
                     LLEWSGTLSEGMLPNRFPDDGDTPEYNSVDASLWFVIAVHDYLSTNHAQADTRTRLQQ
                     AVETILTGYTNGTRFNIKASADDGLLSAGVPGVQLTWMDAKVGDWVVTPRIGKPVEVQ
                     ALWINALRIAATWNPHWQPSAERALQAFHERFTDPSTQALFDNVDVDFVKGTSDRSIR
                     PNQIFAVGGLPFPLLDGAAARAVLDQVEAQLLTPLGVRTLAPSDPAYRGHYSGSPLER
                     DGAYHQGTVWPWLLGPFVEAWLRVHGAGPDARVQAKARFLDPLYAHLDHAGLDHVSEI
                     ADGDAPHTPAGTPFQAWSLGELLRIETLLAGREPIAA"
     gene            5197..8045
                     /locus_tag="VHV99_21795"
                     /pseudo
     CDS             5197..8045
                     /locus_tag="VHV99_21795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018438323.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
     gene            8196..9809
                     /gene="zwf"
                     /locus_tag="VHV99_21800"
     CDS             8196..9809
                     /gene="zwf"
                     /locus_tag="VHV99_21800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX3638065.1"
                     /translation="MSTTPNTPISPRPGAGHEASCKVSASPHDAPPSPAGKRPAPPCT
                     LVIFGAGGDLTKRLLMPALYNLAVDGLLDGGMKIIGVNHGERETSEWREDLHTSLQQF
                     AADKASTFHAGKLDDKAWDWVAQRLEYMAGEFETDDTFTKLKQELDQAPGGNVIFYLA
                     VSSRFFKPIVEHLGTAGLLKEGEGGSGGFRRIVIEKPFGTDLASARDLNAHILSYADE
                     SQVYRIDHFLGKDTVQSILAVRFANALFEPIWRREYIDSVQITAAETIGVEGRGKFYE
                     QTGAFRDMLPNHLFQLLGMVAMEPPNSFDAEAVRDKKAEIFDAILPLTADDVVFGQYE
                     KGPAGVGYREEPDVAPDSTTETYAAARVYVENWRWAGVPFYLRTGKRLAARRTEISVQ
                     LKPVPFRLFRDTPIDALTPNVLTLRIDPAHGTSFDFNVKTPGPVMQIGPVQSSFDYAD
                     FFAEQANVGYETLLYDCMLGDETLFQRADSIETSWCAVDDVLHPKTGGAMPVHGYAAG
                     SEGPAEADALLAQNGHAWRPLQKHAVEKK"
     gene            9941..10699
                     /locus_tag="VHV99_21805"
     CDS             9941..10699
                     /locus_tag="VHV99_21805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX3638066.1"
                     /translation="MLAIDVGGTGLKAAIIDADGQMKTERLRVATPHPCTPEQLVDAL
                     ARLVEPLVEKEPPTLMSIGFPGVVRNNRILTAPHLGVEGWHDIPLADSLAQRLGGLPV
                     RMINDAEMQGFAAIEGHGLEFVLTLGTGAGTALFRDGELMPHLELAHHPVSKKGVAYD
                     EYIGDAAREKVGNKRWNRRVEKVIGILSSLVNYDKLWLGGGNAARLTISLPANVATVS
                     NDAGIEGGARLWHPKSVRETRQLPEANARTGRFK"
     gene            10891..>11238
                     /locus_tag="VHV99_21810"
     CDS             10891..>11238
                     /locus_tag="VHV99_21810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018423926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEX3638067.1"
                     /translation="MCAVLTFAAVSARAADAFALSSPGLADGGTLDSSHAASANNCGG
                     GNVSPALQWRNAPAGTKSYAITIFDPDGAKGLGIVHWVLYGVAPSATGLGAGATPPAG
                     SVGGTNRTGGSGYY"
BASE COUNT         2176 a         3500 c         3496 g         2066 t
ORIGIN      
        1 gttgcttcaa cagcagccaa aattataagt gcgctctctt cttaaaattc actttgttaa
       61 gctaactatc taactttggc caatcggcct aagaccgatg caaaggttcc tcaatgcaac
      121 aaatcaaacg accaggcttc gatcaagcac cccaaacaaa gcagccgccc aaccgaagcc
      181 cagctccttc acccgccctt aattcaccac caacaccggc gccacagccg ccggatcact
      241 aatactcggc cgcccattct ccacatgccc ggccaaacga cgcgagaagc tagcgtcatc
      301 attactcgtc accgtcaagt cataccagtg atggctcgac gccagcaccc aggcctcttc
      361 gatattcgcc ccggccggca ccaacaccgg acgcgtccgc gccccatacg cgttatcagt
      421 caccgtcaac cgcgcaatac cgccgccgcc attgctgaac ttcaaaaaca cattcccgtt
      481 cgcaacatcg tattgcacct tgatctccgg cagcgcgggc ttattgttgc catgccaacc
      541 cgcagcctgc gcggtctgcg ccgtcgcttg ctgcgtatta cccgcaaact tccgcacgaa
      601 accattcggc ccaaacacct cgaacgcata cacaccgttc gtcgtcgtca gatcgaaggt
      661 ctcgctgagc gacttgccgg cctcgacggt ataacgccac ggaccatccg tgcgattcgt
      721 cgcgtacaca tagaaatgcg caccttgatc gccggtattg gcaatcacga tctcgaacgt
      781 gttcttcttc acatccgttt gaccgttcac atgcaactca tacggcaacg cgcgcgcaaa
      841 acgaataccg ctttcctgcg gatcgatcac acccggcgtc gcgggcacgg tcggtttcgg
      901 ctgtgtcgcg cactggttgt cggcgatgct cttgtagttg ctcgtgtccg gcagcggcgg
      961 cactttcgaa tccggcgtgc ggaagtcgaa cgccgtcgtc aaatcgccgc acaccgcgcg
     1021 gcgccattgc gtgatgttcg gctcctgcac gccaaagcgc gtttcgatga agcgaatcac
     1081 cgacgtatga tcgaacacct gcgaacacac aaaaccgccc ttggtccacg gcgacacgat
     1141 cgtcatcggc acgcgcgggc cgaggccgta gggcagaccg tccgcggtat aactaccgcc
     1201 gcgtcccgga ttcaccacgt tgtgaatttc gccatcggtg ctgaccgtcg actggccttg
     1261 cgcgggcgtc gtcgccggtt gcggcggcac gaggtgatcg aagaagccgt cgttctcgtc
     1321 gtacatgatg aacagcacgg tcttgctcca cacctcggga ttcgacgtca atgcatcgag
     1381 aatctgcgac gtgtattccg cgccgtacgc gggcgtatag ctcgggtgct cggagtacgc
     1441 agccggcgga cataaccacg acacttgcgg caacttgttc gccagcacgt cggccttcag
     1501 gtcgtcgatc gtacgcacgg tttgcgcgcg ctcatacaac ggcgaacccg gctgcgcgtc
     1561 gacgaagttc gtgaagttct gcaggatgtt ggtgccgtag ttgccgttca acggatcggc
     1621 gccggtcaaa ccttgctgat acacctgcca cgaaattccc gccgcttgca gacgttccgg
     1681 gtacgtcgtc cacgagagca attgatattt cggcggaccg tcgccgtcga cgaaatcgtt
     1741 gttgtcgagc agcggaccgc cctgcgtgcc ggtcggatcg accatgccgc tcatcagata
     1801 cgcccggttc gggtgcgtcg gtcccgccag cgagcagaag taagcgtcgc agatggtgaa
     1861 cgcatcggcc agcgcgtagt ggaacggaag gtcgctgcgc aggtgatagc ccatcgtcat
     1921 gtcggtcttg ttcgcgggcc actgatcata gcgcccgttg ttaatcgcgg catgggttgg
     1981 ataccagttg tggtcgagat cgccgagaca ttgcgcgctg gtggtcgccg tgttcagatg
     2041 gaacggcagc accggttggg tcgggtcttc cttcgacggt tgataccaca ccggcttgcc
     2101 gcctgggagc ggaatcggga agcggtcgtt atagccacgc acgccgcgta tatggccgaa
     2161 gtagtggtcg aacgatcggt tctcctgcat gaacacgacg atgtgctcga catcgcggat
     2221 cgtgcccgtg cgcgaaaatg cgggcacggc aagtgcgttg cgaatcgact cgggcagcgc
     2281 ggtcagcgcg gcagcagcgc cggcggatga agccacggtc tgcaggaaac ggcgacggct
     2341 agttgatgtc atcgaatatc accttctgcg ggggggaaga atagcgcggc tttgcgcgcc
     2401 agtggaatcg ggtcagttgc tgctggcgtt gtcgcccggc gcgtaatgca tcacgggcgc
     2461 gatttgctgg ctgtcggcag cgaggctggc ttgtatgttt tgcatgatcg gatcggacgc
     2521 agcggcgggc ccggctgcga gcgatgccgg tgcattgaac atgtcggtcg cgggattgat
     2581 tgctgaactt tgcgcggggt cgttgttagc aatgcccggc gataaaggcg tcatcgcggc
     2641 gctttgcgcg gcatcgctgt tggccgcagt cgtcgctgaa gacgaaggcg cggcgtcgtc
     2701 cgcgccacag ccgcttagca aggaagttgc aattgcaaca aaaacggtaa tagcgaggag
     2761 ggcgtgtctt ctcatgtctg catccaactt ggtgatggcg gatgcagtct ctaatccttt
     2821 caagaaataa ttgtgaaacg tttgcgagtg aatcaatatg catcgcaatg tgggttgttc
     2881 gaaaggaatc ggaaagcagt aagcaggaga atggtcacgt tagcgccata cgggcgaagc
     2941 ccaggcacac cgaacgattg gcggcttgca tatcccggta aattcatgac acgcatcgaa
     3001 agacctttcg acatcacgcg tctcgaagac gaatggctcg aggcagacgg cttcggcggt
     3061 ttcgcatcgg gaaccgtcgg cacgctgcgc acgcgccgct atcacgcgtt gctgctcacg
     3121 gccacgcgcg cgcccggcgg ccgcgtggtg cttgtcaatg gtgtggaagc ctggatcgaa
     3181 gcggacggcc agcgttatcc attgagcatg cagcgataca tgccggacgt gatctatccg
     3241 gacctgacgg cgagtcttct cgcattcgac accgacccgt ggccgacatg gcgtttgcaa
     3301 cttgatccgc acaccacgct gatagcggaa gtgttcgtga gcaaggcaac gcgcgaaacg
     3361 gttttgcgtt ggcggttgca gagcgacgag gcggatgctc cagcgaaagc gttcaatctg
     3421 aaagtcaggc ccctgctttc cggccgcgat taccacgcgc tgcaccatga aaaccccgcg
     3481 ttcaatttca acgcgcagac aagcggcgac gcggcatgcg tgaattggca accctacggc
     3541 gacctgccga tcatcaatgc ggtgaccaat ggcacctacg cgcacgcgcc tgactggtac
     3601 cgaaacttct gctacgtgcg cgagcgagag cgcggcctcg atttcagcga ggacctcgcc
     3661 acgcccggcg tattcagctt caacctcgcc gatggcgaag cggtgatgat cctgagcgcg
     3721 tcgaatgcat cgccggcgtc ggcaacgtcg gcaacatctg caacgtctgc aacggaggtg
     3781 gcaggcgcgc aggcagtcgc cgcgtatgca gcggagtcgg tcggcgcagc cgctttttca
     3841 acgagtcagg caggcgcgaa gcccgccgcc taccagacga actctgcgag cacacgaccg
     3901 gccgccgccg acacagcaga ctccgcaacc acaagcccag ccgccgccca cgctacccaa
     3961 ctcgcccaca ccgaacaaca acgccgcagc gcactcggct cccgcctcga acgttccgcc
     4021 gacgcctatg tcgtagcgcg tagcaacggc cgcacggtcc tcgccggctt tccatggttc
     4081 acggattggg gccgcgacac gttcatcgcc atgcgcggcc tgctgatcgc gacgggccgc
     4141 ctcgccgaag ccgaggcgat tctgctcgaa tggtcgggca ccttgtccga aggcatgctg
     4201 ccgaatcgct ttcccgacga cggcgacacc ccggagtaca actccgtcga tgcatcctta
     4261 tggttcgtca tcgccgtcca cgactatctg tcaacgaacc acgcacaagc cgacacccga
     4321 acgcgtctcc agcaggcagt ggaaaccatc cttacgggtt acacgaacgg cacacgcttc
     4381 aacatcaagg catccgccga cgacggcctc ctgagcgccg gtgtccccgg cgtgcaactc
     4441 acgtggatgg acgcgaaggt cggcgactgg gtggtcacgc cgcgcatcgg caaacccgtg
     4501 gaagtgcagg cgctctggat caatgcattg cgcattgcag caacatggaa tccacactgg
     4561 cagccgtccg cggagcgagc gttgcaagcg ttccatgaac gctttaccga tccgtcgacg
     4621 caagcgcttt tcgacaacgt cgacgtggat ttcgtgaaag gcaccagcga tcgctcgatc
     4681 cgcccgaacc agatcttcgc cgtgggcggt ctgccgtttc cattactcga tggcgcggcg
     4741 gcgcgcgcgg tgcttgacca ggtcgaagcg cagttgctca cgccgcttgg cgtcagaacg
     4801 cttgccccgt ccgatccagc ctaccgtggg cactacagcg gctccccgct ggagcgcgac
     4861 ggcgcctatc atcaaggaac ggtctggcca tggctgttag gcccgttcgt ggaagcatgg
     4921 ctacgggttc acggtgcggg gccggacgcc cgtgtacaag ccaaagcgcg ctttctcgac
     4981 ccgctgtacg cccatctcga tcacgcagga ctcgaccatg tgtccgaaat cgccgatggc
     5041 gacgcgccgc acacgcccgc cggcacgccg tttcaggcgt ggtcgctggg cgagttgctg
     5101 cgcatcgaaa ccctgcttgc cggccgggag cccatcgccg cttaaaccgc gctacgatgt
     5161 gagtcccgcc gccaagcccg acgcaaggac ggagtcatgc caccgctgcg cgctgccaat
     5221 ctgctcgaca ccatcgaagg ttcacggctg cattcagccg actgcgccca ttggcagcgc
     5281 tgggggccgt atctcagcga acgtcagtgg ggcacggtgc gcgaggatta cagtccggac
     5341 ggcaccgcct gggactactt tccgcacgac cacgcgcgca gccgcgcata ccgttggggc
     5401 gaagacggca ttgccggctt cggcgacgac aaactcagct ggtgcgtgtc gctcgcgctg
     5461 tggaaccgca aggatccgat catcaaggaa cgtctgttcg ggctcaccaa cgcacagggc
     5521 aatcatggcg aggacgtgaa ggagctgtac ttctacgtcg atggcacacc tacgcattcg
     5581 tatatgcgca tgctctacaa gtatccgcat gccgcctttc cgtacgacga cctggttcgg
     5641 gaaaacgcgc gacgcggcgg cgacatgccc gagtacgaga ttctcgatac cggcgtattc
     5701 gacgatctgc gttacttcga cgtgcaggtc gaatacgcga aacacgcgcc cgacgacatc
     5761 ctgatgcgcg tgacgatcga gaatcgcgcg ggccaggcgg cctcgctcga tgtgttgccg
     5821 cagatctggg cgcgcaattc gtggtcatgg aaggagagca aggacaggcc gtcgctcgtc
     5881 gcgggccagg accacaacgg tgaaacccat gtggtcggcc ggcagcatgg ccatgagccg
     5941 atcgtcgtga cggcgtggtc gaaagacgca ccgcagatgg cgtggctgtt ctgcgagaac
     6001 gataccaacg tcaagcggct gttcaacatg gagggcaccg ggccgttcaa ggacggcttc
     6061 aacgactacc tcgtgcacgg cgaccaacag gcagtacgtc gcgacaccgg caccaaggcg
     6121 gccgcgcatg cgtcgattga actggctccg catggccgcg ccgtggtgta catgcatgga
     6181 cgccagcgca tgtactggct ccgcatggcc gcgccgtggt gtacatgcgc tggcgtccgc
     6241 agtcgtgtcc cgacgataca cagctggatg ccgatgccgt gttcgcccac cgcattgccg
     6301 aggccgatga attctatggc gcgctgcaac acgagatcga cgagcccgat gcgcgcctcg
     6361 tgcagcgtca ggcgctcgcc ggcatgctgt ggtccaagca gtactaccag tacgacgtgc
     6421 accgctggct cgaaggcgat ccgctgcagc ccaaaccgtc cgcgaatcgc aaacgcagcc
     6481 gcaattcgga gtggcagcat ctctgcaacg ccgacatcgt gtcgatgccc gacaagtggg
     6541 aatacccgtg gtacgcgtca tgggacctgg cgtttcacgc ggccgcgttc gcgctgatcg
     6601 atccggcgtt cgccaagcgt caattgctgt tgctggtgaa ggaccgctat cagcatccga
     6661 acggccagct gcccgcctac gaatgggcgc tcggcgacgc gaatccgccg gtgcacgcct
     6721 gggccgcgtg gcgcgtctat gaaatcgacc gcgcgctgac cggcaaggcg gatcgcgatt
     6781 ttctggagct tgtttttcac aagctgttgc tgaatttctc gtggtgggtg aaccgcaagg
     6841 acgcggacgg ccggaatatt tttcagggtg gctttcttgg tctggacaac gtgggcatct
     6901 tcgaccgctc gtcgccgctg cccaccggcg gccatatcga tcagtcggac gggaccgcgt
     6961 ggatggccgc gtacgcgctc gacctgatgc gtatcgcgct cgaactggcg tatgcgaacc
     7021 atgtgttcgt cgatatcggc gtgaagtttt ttgagcactt cctgtatatc gccgaggccg
     7081 tgagttgcga cgacgactgc gagaccggtc tatgggatac cgaagacgaa ttcttctacg
     7141 acaaactgca cctgcccgac ggcaccagcg tgccgatgcg gttgcgctcg atcgtcggcc
     7201 tgattccgct gttcgccgtg cacgtgctcg aagagcgttt gcatggtcac ctgccgggtc
     7261 tgcgcgagcg gctcgtctgg ttcctcgaac atcgtcccga tctggcgaag ctggtctcac
     7321 gctggaatga gcccggcaag ggtaatgcct tgctgctctc gctgttgcgc gggcatcgga
     7381 tgaaagcgtt gctgcgccgt gcgctcgacg aaagcgaatt tctctccgat cacggcgtgc
     7441 gggcgttgtc gcgctttcat cgcgaccacc cgttcgtgtt cacgcacaac gacaccagcg
     7501 tcactgtcaa atatctgccg gccgaatccg acacgcgcgt gttcggcggc aattcgaact
     7561 ggcgcgggcc agtgtggatg ccggtgaact atctgctgat tgaatcgctg tacgaatttc
     7621 atcgctatta cggcgacgat ttccgcatcg agtatccgac cggctccggg cagaagttct
     7681 cactctgcga aatcggcgac aaactggcgc gccgggcgac cacgctgttc ctcaaggaca
     7741 aaaacggtga acgtccggtg atgggcgcgt atccgctctt gcaggccgac ccgcgttcgc
     7801 gcgacctcgt gctgttccat gagtattttc acggcgacaa cggccgtggc gtgggcgcct
     7861 cgcatcagac cggctggacc gggctggtgg cgttgctcct gcaaccgaga gtgatggccg
     7921 cgtcgggcaa tgtgccgcta gccagcgagt cggaccaggc accgattcca acgtcgacca
     7981 cggcgcccgc gcccgagtcc gcgtccgagc ctgcgcctgc gctgacaacc gcggtggcca
     8041 aatagcgcgt ggcgacgcag ccgcgcacga ggttggatag gcgatacagc gcgcggcatg
     8101 gacaggttta gcagttgttt ttgaactgtg cggcttcaag cgtggtcttt catgtaccgc
     8161 cacgtccgtt ccattggctt ccttaagcga atcccatgtc gaccactccg aataccccaa
     8221 tctccccccg ccccggcgcc ggccacgaag ccagttgcaa agtcagcgcg agcccgcatg
     8281 atgcgccgcc ctcgccggcg ggcaagcggc ctgcgccgcc ttgcacgctg gtgatcttcg
     8341 gtgcgggcgg tgacctgacc aaacgattgc tgatgccggc gctctacaac cttgcggtgg
     8401 acggcctgct cgacggcggc atgaagatca tcggcgtgaa ccacggcgag cgcgaaacga
     8461 gcgaatggcg cgaagacttg cacacgtcgc tgcaacagtt cgccgccgac aaggccagca
     8521 ccttccacgc cggcaagctc gacgacaaag cgtgggactg ggtcgcgcaa cggctcgaat
     8581 acatggccgg cgaattcgaa accgacgata ccttcacgaa gctcaagcaa gaactcgatc
     8641 aagcgccggg cggcaacgtg attttttacc tggcggtcag ctcgcgcttt ttcaaaccga
     8701 ttgtcgaaca tctcggtaca gcgggcttgc tgaaagaagg cgagggcggc agcggcggct
     8761 tccgccgcat cgtgatcgag aagccgttcg gcaccgatct cgcgtcggcg cgcgatctga
     8821 acgcgcatat tctgtcctac gcggacgagt cgcaggtgta tcgcatcgac cacttcctcg
     8881 gtaaggacac cgtgcagagc attctcgcgg tgcgctttgc gaacgcgttg ttcgagccga
     8941 tctggcggcg cgaatacatc gacagcgtgc agatcaccgc ggctgaaacg atcggtgtgg
     9001 aggggcgcgg caaattctac gaacagaccg gcgcgttccg cgacatgctg ccgaatcacc
     9061 tgttccagtt gctcggcatg gtcgcgatgg agccgcccaa ttcgttcgat gccgaagccg
     9121 tgcgcgacaa gaaggccgaa atcttcgacg ccatcctgcc gctgaccgcc gacgacgtcg
     9181 tcttcggtca gtacgaaaag ggtccggcgg gcgtgggcta tcgcgaggaa ccggacgtcg
     9241 cgcccgacag cacgaccgaa acctatgccg ccgcgcgcgt gtacgtcgag aattggcgct
     9301 gggccggcgt gccgttttat ctgcgcaccg gtaagcgcct cgccgcgcgt cgcacggaga
     9361 tctcggtgca actgaagccg gtgccgttcc gcctgtttcg cgacacgccg atcgacgcgc
     9421 tgacgccgaa cgtgctgacc ttgcgcatcg atcccgcgca cggtacgagc ttcgacttca
     9481 atgtgaaaac gccggggccc gtcatgcaga tcggcccggt gcaatcgtcg ttcgactatg
     9541 cggacttctt cgccgaacag gccaacgtcg gctacgagac tttgctgtat gactgcatgc
     9601 tgggcgacga gacactgttc cagcgtgccg acagcatcga aacgagctgg tgcgccgtgg
     9661 acgacgtgct gcatccgaag accggcggcg cgatgccggt gcacggctac gcggcgggca
     9721 gcgaaggccc cgcggaggcg gacgcactgc tggcacaaaa cggccatgcg tggcggcctt
     9781 tgcaaaagca tgcagtcgag aagaagtaac tccatgaaac ggatcaacac aatcggggga
     9841 caccgtggcc acacgtaaga cgagcaaaac gagcaaggca ggcaagacca cgaagccaga
     9901 caggacggct gggacaaaag caaccagcag taccgaacgg attctggcga tcgacgtcgg
     9961 cggcaccggc ctgaaggccg cgatcatcga cgcggacggg cagatgaaaa ccgaacgttt
    10021 gcgcgttgcg acgccgcatc cgtgcacgcc ggagcaactg gtggatgcgt tggcgaggct
    10081 ggtcgaaccg ctcgtcgaaa aggagccgcc cacgctgatg tcgatcggct tccccggcgt
    10141 ggtgcgcaac aaccggattc tgacggcgcc gcacctcggc gtggaaggct ggcacgacat
    10201 tccgctggcg gattcgctgg cgcagcgcct cggcggcttg ccggtgcgca tgatcaacga
    10261 cgcggagatg cagggctttg ccgcgatcga aggtcatggg ctcgagttcg tactaacgct
    10321 cggcacgggc gcgggcacgg cactgttccg cgacggcgag ttgatgcctc acctcgagct
    10381 tgcgcatcat ccggtcagca agaagggcgt ggcgtacgac gaatacatcg gtgacgcagc
    10441 gcgtgagaag gtgggcaata agcgctggaa tcgccgggtc gagaaggtga tcgggattct
    10501 gagttcgctg gtgaactacg acaagctgtg gcttggcggc ggcaacgcgg cgcgcttgac
    10561 gatcagcttg cccgccaacg tcgcgactgt ttcgaacgat gcgggcattg aaggcggcgc
    10621 gcgcctgtgg catccgaagt ccgtgcgtga gacgcggcag ttgccggaag ccaatgcgcg
    10681 gaccggccgg ttcaagtgat acgtgattgc cgggcggatt ctttgcacgt cggcggaata
    10741 aagggtttga tcgatacgtt tgctaccgga ggtagtcacg cacatccgtt gcgtgactgt
    10801 ccttttcaag tcggctaact tatacccggg caatcaacaa tgattcgaag cgataacgct
    10861 ggcgttgcac gacgccttct atctgtcggt atgtgcgccg tgctgacctt tgccgctgta
    10921 agcgcccgcg cggctgacgc gttcgcgctg tcgtcgcccg gtttggccga cggcgggacg
    10981 ctggactcga gccatgcggc gagcgcgaat aattgcgggg gtgggaatgt gtcgcccgcg
    11041 ctgcaatggc ggaatgcgcc tgcgggaacg aagagctacg cgatcacgat tttcgatccg
    11101 gacggcgcga agggattggg gattgtgcac tgggtgctgt atggcgtcgc gccctcggcg
    11161 accgggctgg gtgctggcgc tacgccgccg gccggcagtg tcggcggaac gaatcggacg
    11221 ggcggctcgg gttattac
//
LOCUS       DATASH010000813         3999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1521793,
            whole genome shotgun sequence.
ACCESSION   DATASH010000813 DATASH010000000
VERSION     DATASH010000813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1521793"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..732
                     /gene="rsmD"
                     /locus_tag="VHV99_21815"
     CDS             118..732
                     /gene="rsmD"
                     /locus_tag="VHV99_21815"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEX3638068.1"
                     /translation="MPRSAPSRAHGASSKGGKPHAIRIIGGDWKRTPLPVLDLDGLRP
                     TPDRVRETLFNWLGQRLDGQRCLDLFAGSGALGFEAASRGAARVLMVERNARAASQLR
                     ANQERLAARSIEIAEADGLRLAASLAPGSFEVVFLDPPFGDDLLDKALALAAPLLSPE
                     GFLYVEAGAALELDGNETLAGWEIVRHGKAGAVHFHLLQRENKE"
     gene            844..1359
                     /gene="coaD"
                     /locus_tag="VHV99_21820"
     CDS             844..1359
                     /gene="coaD"
                     /locus_tag="VHV99_21820"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEX3638069.1"
                     /translation="MVVAVYPGTFDPLTRGHEDLVRRASSIFDTLVVGVADSRNKKPF
                     FTLEERLDIAHEVLGHYPNVQVMSFKGLLKDFVRTNNARVIVRGLRAVSDFEYEFQMA
                     GMNRYLLPDVETMFMTPSDQYQFISGTIVREIAQLGGDVSKFVFPSVEKRLKEKVAAM
                     DSNDGVSAGQP"
     gene            1423..1680
                     /locus_tag="VHV99_21825"
     CDS             1423..1680
                     /locus_tag="VHV99_21825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013337947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HEX3638070.1"
                     /translation="MALMITDECINCDVCEPECPNDAISMGPEIYVIDPKKCTECVGH
                     FDEPQCIQVCPVECIPRDPEHLETGEGLMAKYHALQAAKSV"
     gene            complement(1929..3008)
                     /gene="hisC"
                     /locus_tag="VHV99_21830"
     CDS             complement(1929..3008)
                     /gene="hisC"
                     /locus_tag="VHV99_21830"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX3638071.1"
                     /translation="MSRYWSDVVHRLTPYVPGEQPVAAHPVKLNTNENPYPPSPRVLE
                     AIRQELGATGGSLRRYPDPTARKLRETVAAYHCIRPEHVFAGNGSDEVLALTFKALLK
                     HDKPLLFPDITYSFYPTYARLFEVDYRTIALDDSFGINVDDYTAPNGGVLFPNPNAPT
                     GRPLPLGEIERLLARNTESVVVIDEAYVDFGAESAIVLIDRYPNLLVIQTVSKSRSLA
                     GMRVGFAFGNPELIDALTRVKDSFNSYPLDRLAQVAAVAAYEDDAWFRENCAKVMASR
                     ERLAAGLTAVGFEVVPSAANLLFARHEGYDAAMLVARLTEKEIFVRHFKAARIDQHLR
                     ISVGTDAECDLLLDALRDIFSAYAG"
     gene            complement(3091..3693)
                     /gene="pth"
                     /locus_tag="VHV99_21835"
     CDS             complement(3091..3693)
                     /gene="pth"
                     /locus_tag="VHV99_21835"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX3638072.1"
                     /translation="MIKLIVGLGNPGAEYTATRHNAGFWLVDQLAREAGTTLRDERRF
                     HGFYAKARLHGEEVHLLEPQTYMNRSGQSVVAVAQFFKILPDEILVAHDELDMPPGGV
                     KLKLGGGSGGHNGLKDITAHLSSQQYWRLRIGIGHPRDLIPESARAGAKPDVANYVLK
                     PPRREEQDVIDASVERALAVMPQIIKGELERAMMQLHRNP"
     gene            complement(3835..>3999)
                     /locus_tag="VHV99_21840"
     CDS             complement(3835..>3999)
                     /locus_tag="VHV99_21840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEX3638073.1"
                     /translation="GHSLHAKDIVLPAGVALVAHVEAENPVIAAATIPAGAIAEGDAA
                     AAGEGETPAA"
BASE COUNT          771 a         1197 c         1245 g          786 t
ORIGIN      
        1 ccaccagtcg tattggccga acggcaaggc tggacgccgg gcgcccctgt ggtaacgttg
       61 gcgctcagtc cgcgcgcatc ggcgtcgcgg gcgccctaat aacgtaatcg aaactgcatg
      121 ccccgttctg ctccttcacg cgcccacggc gcttcgtcca aaggcggcaa gccgcacgcc
      181 atccgtatca tcggcggcga ctggaagcgc acaccgctgc ccgtgctcga cctcgacggc
      241 ctgcgcccca cgcctgaccg cgtgcgcgag acgctcttca actggctcgg ccaacggctg
      301 gacggccagc gctgcctcga tctgtttgcc ggcagcggtg cgcttggctt cgaagctgcc
      361 tcgcgcggcg cggcgcgggt gttgatggtc gagcgcaatg cgcgggccgc cagccagctg
      421 cgcgcgaacc aggagcgcct cgcggcgcgt tcgatcgaaa tcgcggaagc cgacggcttg
      481 cgcctcgcgg cgagtctcgc accaggatcg ttcgaggtgg ttttcctcga tccgccgttc
      541 ggcgacgatc tgctcgacaa ggcactcgcg ctggccgcgc cgctgttgag cccggaaggt
      601 tttctgtacg tggaagcggg cgccgcgctc gaactggatg ggaacgagac ccttgccggc
      661 tgggaaatcg tgcggcacgg caaggcggga gccgtccact ttcatttgct gcaacgcgaa
      721 aataaggaat aatgcgcgtt caaaacaagc tgcgggcggg ttaccgtcac gcatgcggtc
      781 ggtgccaaat gcgtcgcgtg gacgctttga cgtgcccatt tgtctgaaga gaggagaagt
      841 ctcatggtag tcgccgtgta cccgggcacg ttcgacccgc tgacgcgcgg tcacgaagac
      901 ctcgttcgac gcgcgtcgag cattttcgac acgctggtgg tgggcgttgc cgacagccgc
      961 aacaagaagc cgttcttcac actggaagag cgtctagaca tcgctcatga agtactcggg
     1021 cactacccga acgttcaggt gatgagcttc aaagggctat tgaaggactt cgtccgcacc
     1081 aataacgcac gagtgatcgt gcggggcctg cgcgctgtct cggacttcga atatgagttc
     1141 cagatggccg gcatgaatcg ctatttgttg ccggacgtcg aaaccatgtt catgacgccg
     1201 tccgatcaat accagttcat ctccggcacc atcgtgcgtg aaatcgcgca actgggtggc
     1261 gacgtcagca agttcgtgtt cccgtcggtt gaaaaacggc tgaaagagaa ggttgctgca
     1321 atggattcga atgacggtgt ttcggcgggg caaccgtaac cggcgtaaac tgtcggtttg
     1381 acggatcaaa gtcggcttcg gccggcgcga agtgagagaa gtatggcctt gatgattacc
     1441 gacgagtgca tcaattgcga tgtgtgcgag cccgagtgcc caaacgacgc gatttcgatg
     1501 ggcccggaaa tctacgtgat cgacccaaag aaatgcaccg agtgtgtcgg ccatttcgat
     1561 gagccccagt gtattcaggt gtgccccgtc gagtgcattc cgcgtgaccc cgagcatctg
     1621 gaaacgggag aaggtttgat ggcgaagtac cacgcgcttc aggctgcaaa gagtgtttga
     1681 tctggaagtg ccagcgcttc atgctgcaaa gagcgtttga tctggaagtg ccaacgcttc
     1741 agactgcaaa gagcgtttga tctggaagtg ccaacgcttc aggctgcaaa gagtgtttga
     1801 tctggaagtg ccagctcttc agactgtaaa gagcacgtgg gatttaacga tggtgaacgg
     1861 cgagacgcat gcctcgccgt tttcttttgg tgcggcgctc ttgtgttgcg cgtgcgattt
     1921 tccactcgtt atccggcgta agcgctgaaa atgtcgcgca gcgcatccag cagaaggtcg
     1981 cattcggcgt cggtaccgac cgagattcgc agatgctggt cgattcgcgc cgccttgaaa
     2041 tggcgcacga agatttcctt ctccgttagc cgtgccacaa gcatcgccgc atcgtagcct
     2101 tcatgacgcg cgaacagcag attcgccgcc gacggcacca cctcgaagcc cactgccgtc
     2161 aagcctgccg ccagccgttc acgactcgcc atgaccttgg cgcagttttc tcggaaccac
     2221 gcatcgtcct cgtatgccgc gacggccgcg acttgcgcca gacggtcgag cggatatgag
     2281 ttgaagctgt ccttcacgcg ggtcagtgcg tcgatcaatt ccggattgcc gaaagcgaag
     2341 ccgacgcgca tgccggccag cgaacgcgac ttcgatacgg tttggatcac cagcaggttg
     2401 ggatagcggt cgatcagcac gatcgccgat tcggcgccga agtcgacata ggcctcatcg
     2461 attaccacga ccgattcggt gtttctcgcc agcagacgct cgatttcgcc gagcggcagt
     2521 ggccgtccag ttggcgcatt cggattgggg aacagtacgc cgccgttggg cgccgtgtag
     2581 tcgtcgacat tgatcccgaa gctgtcgtcg agtgcgatgg tcctatagtc gacttcaaag
     2641 agccgcgcgt aggtcggata gaagctgtac gtgatatccg ggaacagcaa cggtttgtcg
     2701 tgcttgagca atgccttgaa ggtgagcgca agcacttcgt ccgagccgtt gccggcaaac
     2761 acgtgctccg ggcgaatgca gtggtacgca gcgaccgttt cgcgcaactt gcgcgccgtc
     2821 ggatccggat agcgtcgcag cgacccgccc gtggcgccca attcctgccg gatcgcttcg
     2881 agcacgcgcg gcgacggcgg atagggattc tcgttggtgt tcagtttcac cggatgcgcc
     2941 gccacgggct gctcgcccgg tacataaggc gtcagacggt gaacgacatc actccagtag
     3001 cggctcaagc gattctcctg tcgatggcga aacggcgaaa cggcgaaacg gcgaaacggc
     3061 gaaacgggcg cgtagggcgc gtagggccgc tcagggattg cgatgcagtt gcatcatcgc
     3121 ccgttcaagc tcgcctttga tgatctgcgg catcacggcc aaagcacgtt cgaccgacgc
     3181 gtcgatcaca tcctgttctt cccgccgcgg cggtttcagc acgtaattgg cgacgtccgg
     3241 cttggcgccg gcgcgcgcgc tttcgggaat cagatcccgc ggatggccga tgccgatccg
     3301 caaccgccaa tactgttgcg acgacagatg cgcggtgatg tccttcagcc cgttatgccc
     3361 gccgctgccg ccgccgagct tcaacttgac gccgccgggc ggcatatcga gttcgtcgtg
     3421 cgcgaccaga atttcgtccg gaagaatctt gaagaactgc gcgaccgcca cgaccgactg
     3481 ccccgagcgg ttcatatacg tctgcggttc cagcagatgc acttcttcgc cgtgaagccg
     3541 ggccttcgcg tagaagccgt gaaagcgccg ctcatcgcgc agcgtcgtgc ccgcttcgcg
     3601 cgccagttga tcgactagcc agaagccggc gttgtgacgt gtcgcggtgt attcggcgcc
     3661 cggattgccg agcccgacga tcagcttgat catgattgcg taggtccatc tggctctgag
     3721 ccgaacgcag ccgagcctaa aaaaccgaaa aaaaaacccg ccggggcgaa cctcggcggg
     3781 tcacatcgac cgtttcattg aaacggatcg agaggattgc tcgctcagtg cagcttaggc
     3841 agccggcgtt tcgccttcgc ctgcagcagc agcgtcgcct tcagcgattg cgccagccgg
     3901 gatcgttgca gctgcgatga ccgggttttc tgcttcaacg tgagcaacca gtgcaacgcc
     3961 tgccggcagg acgatgtcct tggcatgcag cgagtgacc
//
LOCUS       DATASH010000814         7688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6070014,
            whole genome shotgun sequence.
ACCESSION   DATASH010000814 DATASH010000000
VERSION     DATASH010000814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7688
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6070014"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(498..881)
                     /locus_tag="VHV99_21845"
     CDS             complement(498..881)
                     /locus_tag="VHV99_21845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013094374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638074.1"
                     /translation="MEFDSDWLTLGRHRVRLRSSRGFPTELMRSVAEVVRLAIDNNMS
                     ARARLVEIVFQHEQAYDIAVGTTLAEDRVCAPQLEAAIAVVLGLLPQQVNITVTTVSQ
                     DEVDLHFGVYERMLAEKVGVVPPIQ"
     gene            1125..1577
                     /locus_tag="VHV99_21850"
     CDS             1125..1577
                     /locus_tag="VHV99_21850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006751146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX3638075.1"
                     /translation="MSDLFSGTDLFSEFDRLQRQMDSLFGGFPSSIRSGRFGAFPQVN
                     IGTTDDSIEIVAFAPGLKLADLDLSIDKGLLTISGERKAAQVGSDDQTRGDTRTYAQE
                     RFTGSFRRVIELPQNADPDKVQAHYTNGCLSITVGKREASKPRAITVQ"
     gene            1614..2021
                     /locus_tag="VHV99_21855"
     CDS             1614..2021
                     /locus_tag="VHV99_21855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX3638076.1"
                     /translation="MNDRTQVAERDQKPVARREGEQPARRMTLTPAVDVFEDSQGITL
                     WADLPGVTRDKLDVKVHDGNLYIEAEAVVPTPAGLRLQHAEIREPHFARAFSLSPDFD
                     TSKIDANLQDGVLKLTIPRRDEARPRRIEVQTS"
     gene            2459..3022
                     /locus_tag="VHV99_21860"
     CDS             2459..3022
                     /locus_tag="VHV99_21860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006759512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX3638077.1"
                     /translation="MNPDLKKWNPFKFLRGSARRQDTDSAQSGPGSESSRVPWPDIPR
                     LFSRDPWRAMEDFFHDPFAARGALERWFGDFSSSRFQPRIDVVDEGKVLRVTVELPGM
                     EREDVTVSVEDSALVLHGEKRQDVHSEEDGCYRLERAYGTFTRTIPMPENADPDHALA
                     KFDRGVLTLTVPKQEQQRSASRTIDIG"
     gene            3068..3140
                     /locus_tag="VHV99_21865"
     tRNA            3068..3140
                     /locus_tag="VHV99_21865"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3101..3103,aa:Lys,seq:ttt)
     gene            complement(3322..3648)
                     /locus_tag="VHV99_21870"
     CDS             complement(3322..3648)
                     /locus_tag="VHV99_21870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638078.1"
                     /translation="MTKDTIPEKRYTLIVALLNRHAVRARHDLADTFVRRMAASHNRA
                     NEELEVVQRSQRTQADVLVVLPEGVVEILVDQSDKTTIANRSANGSRPRAPQTVSREL
                     RRSARS"
     gene            complement(3723..3866)
                     /locus_tag="VHV99_21875"
     CDS             complement(3723..3866)
                     /locus_tag="VHV99_21875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638079.1"
                     /translation="MIALLARDLASRQTGYNRIKRHAKRPSRQHLDLLIDRITCLDEP
                     ATF"
     gene            4348..5496
                     /locus_tag="VHV99_21880"
     CDS             4348..5496
                     /locus_tag="VHV99_21880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007589659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX3638080.1"
                     /translation="MRLADFILRDMDAILARWEAFAATLLPAARKMNSLALRDHAQPL
                     LEAVAADLLTLQTREAQLHTSMGPASRPTDAPQTAAQTHAILRAQGGFNINQLVAEYR
                     ALRASVLGLWMDDCRPEVPHPHDIIRFNEVIDQALAESVGSFSEQVEQARNLFLGMLG
                     HDMRSPLQTIQATALYLARLNAGDSVSDAAGRLIRTGARMQALLDDLCDFSRTRLGVG
                     IDITPARIDLAEIFADELDQLRAVHPDRRIVLEASGGGFQGIWDGPRLQQLLGNLVLN
                     AIKYGASDTPVRVVLTGDETDVQFEVRNAGPTVEPATLQRIFDPLERGPDAPQRHDGS
                     LGLGLFISRAIASAHGGTVDAHSAGPETVFTVRLPRQQEGPSPRERRH"
     gene            6162..6359
                     /locus_tag="VHV99_21885"
     CDS             6162..6359
                     /locus_tag="VHV99_21885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638081.1"
                     /translation="MTRRNDWTRDTSSEYISRFVDTFARGSFCGMRVSDFAVLEPFEV
                     DGVPVFPPVFGVRIPTIVRRQ"
     gene            6751..7254
                     /locus_tag="VHV99_21890"
     CDS             6751..7254
                     /locus_tag="VHV99_21890"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEX3638082.1"
                     /translation="MNVTTLKYRQLRPGSSGRLKVYDWMVGAVAELEKVASLNSVAVR
                     TSLGERRVSAEALAYSQSVREAIALQSEELHAALAKTFACCRRIEEITAENGGLLASV
                     MLKNVQTLVATTSALIGVQPAENGQQTMSEAVTKSLPGLVARRKIVPEPGSTAFFAER
                     VPRRHMH"
BASE COUNT         1441 a         2313 c         2455 g         1479 t
ORIGIN      
        1 gaaacacgaa gtccggcgtc ggtgtaaagc ggattccgga atccggaccg atgacgcgtc
       61 ccctgacggc gacatgctgt cccagataag gtagcaggag tttcgggtcc gtggcgccga
      121 cgttgccgag caggatgtcg gcacgacctt ctggtcgcaa ggcgaggccg ccgggcggtc
      181 cagcagcagg gttgtgtagt cacccggcgc gtattgtccg ttgccttgct gtatcgtgcc
      241 gccgatccag atgtcctgct gcaggggcaa cgttttcagg ggtggcccgg aaggtaccgc
      301 catggcaggc atgaaccggc aatcagcacg gctgccgcga gccgtgggcc tgacaggttt
      361 ttcatcatga ggctccctgt tcgttctccc gtccccgtcc cggtcgatga tccgggtcgc
      421 tccgaacggg caccaggcgg gcattcgggt tgcccgtcgc tgtgccctct ctccggattg
      481 aagcggtcac ccgacgatca ctggatcggt ggcaccacgc cgaccttctc ggcgagcatg
      541 cgttcataga ccccgaaatg caggtcaact tcgtcctggc tcacggttgt gaccgtgatg
      601 ttcacctgtt gcggcagcag gccgagcacc accgcgatcg ccgcttccag ttgcggcgca
      661 caaacgcggt cttcggcaag cgtcgtgccc accgcgatat cgtaggcctg ttcgtgctgg
      721 aagacgatct cgaccagccg cgcgcgggcg ctcatgttat tgtcgatcgc gagccgtacg
      781 acctccgcca cgctacgcat caactctgtc ggaaagcccc ggctcgagcg cagccggacg
      841 cggtgtctgc caagcgtgag ccagtccgaa tcgaattcca tggtgtcctc ctgttgtcgt
      901 cgcaatgcac cgcgataata ggatctggca ggcgattttc aagaccggaa ttccatcacc
      961 accccttgaa attttcgcca gaggtcctat cttggatatc gctcaggcag acgtgccggt
     1021 cacatccggt gcgctgcgtg tttcgatttg tttgccagcg gcctggcagg cggactggag
     1081 cgcgtaggcc cgttcgcctc ctcgctgaat cgaaggagga tacgatgagt gatctctttt
     1141 ccggaaccga cctgttcagt gagtttgacc gtctgcagcg gcagatggac agcctgttcg
     1201 gcggttttcc gtccagcatc cgctcgggcc gtttcggggc cttcccgcag gtcaatatcg
     1261 gtacgaccga cgactcgatc gaaatcgtcg cgttcgcgcc cggtctcaag ctggccgatc
     1321 tcgacctgtc gatcgacaag ggcctgctga ccatcagcgg tgagcgcaag gccgcccagg
     1381 tcggcagcga tgaccaaacc cgcggcgaca cccgcaccta tgcgcaggag cgcttcaccg
     1441 gcagtttccg gcgcgtgatc gaactgccac agaatgcgga ccccgacaag gtgcaggcgc
     1501 actacaccaa cggctgcctg tcgattaccg tcggcaagcg cgaagcgtcg aagccgcggg
     1561 ccatcactgt ccagtaaccc gtccggtaat gtcagccatt caaggagcaa gccatgaacg
     1621 acaggaccca ggtcgcggaa cgcgaccaga aaccggtagc acgacgcgag ggtgagcagc
     1681 cggcacggcg gatgacgctc acaccggccg tcgacgtgtt cgaggacagc cagggcatca
     1741 cgctgtgggc ggacctgcca ggcgtcacca gggacaaact cgacgtgaag gtgcacgacg
     1801 gcaatcttta tatcgaggcc gaagcggttg tgccgacgcc cgccggtctg cggctgcagc
     1861 acgccgagat ccgtgaaccg catttcgcgc gcgcgttctc gctgagcccg gacttcgaca
     1921 catcgaagat cgatgcaaac ctccaggacg gcgtgctgaa gctgaccatt ccgcgtcgcg
     1981 acgaagcacg cccgcgccgg atcgaagtgc aaacgagctg agcccggcag agccgcaacg
     2041 acgacaggag gaaaaccggt ggcggccgca ggtcgccacc gcccggttcg tggccgcgcg
     2101 gatgcagaac gtctggagcg cgaatacacg atcgggacat ggcgccctgt ggacatgccc
     2161 ggttcgtagg cggcctgccc gtccctgatt gatctgtcgt gctgacccga accaggcgcg
     2221 atgcccggct gctggtccag tggcatccgc cgcgggtcac tcagcgcggg actggccgct
     2281 gaggccgggt gcaatgcccc gccgcacgcc gtcaggctga ctgcaaacgg tgtatgcgcg
     2341 agcgccaagg caggcaacgg tggccgcctg ggccagtccg ggaacgcagc agaaacgttg
     2401 cggtgaaagc ccgatgacgg acatccggct ctcctgtcag accatcgagg agcatacgat
     2461 gaaccccgac ctgaaaaagt ggaacccttt caaatttctc cgcggttcag cacgcaggca
     2521 ggacacggac agcgcgcaga gcgggcctgg cagcgagtcg tcacgcgttc cgtggcctga
     2581 cattccgcga cttttctccc gtgatccgtg gcgtgcgatg gaagatttct ttcatgatcc
     2641 gttcgcagcg cgcggcgcgc tcgaacgatg gtttggcgac ttcagttcat cgcgtttcca
     2701 gccgcgcatc gacgtagtgg acgaggggaa ggtactgcgc gtgaccgtcg agctgccagg
     2761 catggagcgg gaggatgtga ctgtcagcgt cgaggacagc gcgttggtat tgcacggcga
     2821 gaaaaggcag gatgtgcaca gcgaggagga tggctgctat cggcttgagc gcgcatatgg
     2881 aaccttcacg cgcacgatcc cgatgccgga aaatgctgat cccgaccacg ctctcgcaaa
     2941 gtttgacagg ggcgtgctta cgttgaccgt gccgaaacag gaacagcagc gatccgcgag
     3001 ccgcacgatc gatattggct agcatcgggg gcatgacgct ggcgcggcac atgaatggga
     3061 gtcgctcggg tcgttagctc agcaggtaga gcagcggact tttaatccgt tggtcactgg
     3121 ttcgaatcca gtacggccca actccccgat gcgtctgccg cgggcagtga cgggcccacg
     3181 aattgacaca tgcatacgcg tcgaaggcag gagcgatacc acagcggaac agatggattt
     3241 gtggatttgt cctggctggt gcaggtcagc aggaaagccg cttccgtctt gtgacgcgtt
     3301 cattcgtgat aattgccgcc gctaactccg cgcactgcgg cgcaactctc gcgaaacggt
     3361 ttgaggtgct cttgggcgcg agccatttgc agatcgattc gcgatggtgg tcttgtcgga
     3421 ctggtcgacg aggatttcga ccacgccctc aggcaggaca acgaggacat ccgcctgcgt
     3481 gcgctgcgag cgctggacta cctccagttc ttcgttcgcc cgattgtgac tagctgccat
     3541 gcggcgcacg aacgtgtccg ccagatcgtg gcgggcgcgc accgcgtgcc ggttcagcaa
     3601 tgccacaatc agcgtgtagc gcttctcagg gatggtgtcc tttgtcacat tttccgcgtc
     3661 caggctgacg gcccgggtcg ccagcgaccg cagtttcgac gcaggaatgc cagcgatggc
     3721 atttagaaag tcgccggttc atcgagacag gtgatccggt cgatcagcag atcgagatgc
     3781 tggcgcgacg ggcgtttcgc gtggcgcttt atccggttgt agccggtctg gcggctcgcc
     3841 agatcgcggg caaggagggc gatcaagagc acgcctctgc tcgacggtga gcccgctcgg
     3901 caacacgccg gaacagccgc gtctgggtgt cacattgacc tgcttgtgat tcggtcccag
     3961 cgtcgggagc gccggaagtt caccactctg cgcgatcagc tcatgactcg tggcgttgat
     4021 aatgtcgact ggctggtcga tcgatggagc cgccgtatgc gccgtgcgtg tgcgcatgac
     4081 gctcgcctcg tagcagggtt cgaccgacca atcttaccga ttagttcgtc ggcagattta
     4141 cgcgcgcgat aatcggcata cgacagttgt gctaggccta acctgatatc gaggatgggc
     4201 cgtgtcgaag aggcgatcgc gttccacttc ttccggagtt gcgagtcacc gctggcgtaa
     4261 ggcgtggcgt cgtgccccag tggcgagtaa aatgcatggt gacataagct gaactggcgg
     4321 ctttccgctt ttatataagg gactggcatg cgactggcgg attttatcct gcgcgacatg
     4381 gatgccattt tggccagatg ggaggcgttt gctgccacgc tgctgccagc ggcgagaaag
     4441 atgaattcgc tggctttgcg cgaccatgca cagccgctac tggaagcggt cgccgccgat
     4501 ctgttgacgt tgcagacgcg cgaagcacaa ctgcatactt ccatgggacc ggcatccaga
     4561 ccgactgatg ccccgcagac tgcggcgcag acgcacgcaa ttctgcgtgc tcaaggaggg
     4621 ttcaacatca accagctcgt cgccgaatac cgtgcgctgc gcgccagcgt gctcgggttg
     4681 tggatggatg actgccggcc cgaagtgccc catccgcatg acatcatccg tttcaatgaa
     4741 gtgatcgacc aggcacttgc cgaatcggtc ggttctttca gtgagcaggt cgagcaggcc
     4801 cgcaacctgt tcctggggat gctgggccac gacatgcgca gcccgctgca aaccattcag
     4861 gcgacggctt tgtacctggc gaggctaaat gcgggtgaca gcgtgtcgga tgcggcaggc
     4921 cgtttgatcc gcaccggtgc ccgcatgcag gcactgcttg acgatctgtg tgatttcagc
     4981 cggacgcgac tgggcgtggg aatagacata acgccggccc gtatcgacct cgcagaaatt
     5041 ttcgccgacg agctagacca gttgcgcgcc gtgcatccgg accgccggat cgttttggag
     5101 gcatccggcg gcggctttca gggtatctgg gacggcccgc gcctgcagca gctgctcggc
     5161 aaccttgtcc tgaacgcgat caagtatggc gcgtccgaca cgccggtgcg ggtggtgctg
     5221 acaggcgacg agacggacgt ccagttcgag gtaagaaatg cagggcctac ggtcgagcca
     5281 gcgacgctgc agaggatatt tgacccgctc gagcgcggcc ccgatgcgcc acagcggcac
     5341 gacggcagtc tggggctcgg cctgttcatt tcgcgggcga tcgcctccgc gcacggcggc
     5401 acggttgacg cccactcggc ggggcccgaa accgtgttca cggtacgcct tccccggcag
     5461 caggagggac cgtcaccgcg tgagcgacgt cactaacgcg accagctggc tggatggccg
     5521 ccctttccgc atgtagccgt cgaattcgac gccggtagcc tgccggcaca ggcgttcgtc
     5581 cggcataagt tcggaaggtg ccatctcggg gcgcagatca aaaacgccat accgtatagc
     5641 tgtctgtcac tgcggcggcc ttcaaacatc cccttgtgcg gatattggtc agcgcggcga
     5701 cgtcccgcgc ctagacagtc gtgtcggccg gtgagtttcg cgttgcagcg gtgttcgcgg
     5761 ctccatgagg tggcttccgc ggacttcagg ctgaacagag ccttcggtgg gtggtacagc
     5821 ctgtcactgc gccgagcagt aatcgaagca tgccaatgac gaagcctgaa ctgatcggga
     5881 ggtatgccgt gtgtttgcaa gcgtgctcaa cgcggcgtca atatgcgtgt ggacgaaaac
     5941 atcggaggtg tcgcaccgtt cgaggccggt gctatcggaa tgaagggccg ggctgattga
     6001 tggcgtatgg tgccgtgctt gactccggca tgcagaaccg ttcggccggg tctaatctgg
     6061 tttgataaag ggagccgggg cgcgaccggg cgatgcccga gtcccgagag ccaatggaga
     6121 cggagaactg aaaatttaca cattgtgcat gctcgcccgt catgacaagg cgtaatgact
     6181 ggacacgcga cacgtcctct gaatacatct cgcgtttcgt tgacaccttc gcgcgcggat
     6241 cattctgcgg catgcgggtg agcgatttcg ccgtgctcga gcccttcgaa gtggacggtg
     6301 taccggtgtt cccgccggtg ttcggcgtgc gcataccgac cattgttcgg cgacagtgac
     6361 ccccgcgtgc gcatgcgtcc gttaccccct tggggaacgt tcgctcaagg cgctgtcagg
     6421 atcaccgggc cgaatgatgg aatgatggcc cgcctgggtc gcccctcacc cgccccttga
     6481 aaccatcgcg ggagctcccc ctcaaagccg tagatttgca tgaaatgtta cggccgatgc
     6541 tttcgcggtt gtcggcgttt gcattcacct accacgatac cgatggcacc tcgggaacgt
     6601 ccgttgcgta cgccactttc gagatcgcct gcaagccggc tccacgcgac gccgcgctcc
     6661 caccaatcca gcatccagcc atcggtgacc cgttgacggc tgcttccgcc acgcgggcgt
     6721 aggcctgcgt cctcctttcc ggagcgttcc atgaatgtca ctactctcaa atatcgtcag
     6781 ctccgccccg gcagctccgg caggctgaaa gtctacgact ggatggtcgg cgccgtcgct
     6841 gagctcgaga aagtggcaag tctgaattca gtagccgtta ggacgtcgct cggcgaacga
     6901 agggtttcgg cagaggctct ggcctattcg cagtcggttc gtgaagcgat tgctctccag
     6961 tcggaggagc tgcacgccgc actggcgaag acttttgctt gttgccggcg catcgaagag
     7021 atcactgcag aaaatggcgg tctcctcgcg tcagtaatgc taaagaatgt ccagactttg
     7081 gtggcgacaa cgagcgcgtt gattggcgtt cagccggcgg aaaatgggca gcagacaatg
     7141 tcggaggcgg ttacgaaaag tctaccgggg ctcgttgccc gcaggaaaat tgtgcccgaa
     7201 cccggatcaa cggcattctt cgccgagaga gtgccacgcc ggcacatgca ttgagagttg
     7261 gttcgagcat tatgctggaa tgcaaacgcg ctgggggatc tcggcatttc tgccgaccgc
     7321 acgtcggcct gtgccgcgac gatcagtgag caaggaccgt gcgcttcgcc ccgccgagcc
     7381 ggacggtcac cgcggcgttg cgcgactgcg gtttcccgta cgagggcgag gagatcgagg
     7441 ccgtcgtcga cagcatctgc attgcgcact tcccggaaga gacggccgcg gtcctgagga
     7501 tgtcgatccg aatgcctcat gcagccagat tgtgcgagac attactgata tgtggaggca
     7561 ggaggtatgt ccagatctag tcctctacag tggaccagac ggtccgcttt tgctaactat
     7621 gcggttgcaa ttctgtcggt tgctctggca ctcgtcgccg cgctgctgtt agaaccgcac
     7681 acgcagag
//
LOCUS       DATASH010000815         1644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4884660,
            whole genome shotgun sequence.
ACCESSION   DATASH010000815 DATASH010000000
VERSION     DATASH010000815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4884660"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VHV99_21895"
     CDS             complement(<1..494)
                     /locus_tag="VHV99_21895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX3638083.1"
                     /translation="MSHSLRVIFAGTPEFAAAALAAIHGAGFPVPLVLTRQDQPAGRG
                     MKLQASPVKRYAQEHGIDVAQPPSLRRTGKYPVEAAAAIDQLRATPHDVMVVAAYGLI
                     LPQEVLDIPPLGCINIHASLLPRWRGAAPIHRAIEAGDTETGITLMQMDVGLDTGAMI
                     SEART"
     gene            complement(526..1029)
                     /gene="def"
                     /locus_tag="VHV99_21900"
     CDS             complement(526..1029)
                     /gene="def"
                     /locus_tag="VHV99_21900"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010100727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX3638084.1"
                     /translation="MALLNILNYPDKRLHKIAKPVEVVNDRIRRLVADMAETMYAAPG
                     VGLAATQVDVHERVIVIDVSETHDELLAFINPEIIWSSDERKLSEEGCLSVPGIYDNV
                     ERAEKVRVRALNQKGEAFELDCEGLLAVCIQHEMDHLMGRVFVEYLSSLKQTRIKSKM
                     KKLAHAM"
     gene            1273..>1644
                     /locus_tag="VHV99_21905"
     CDS             1273..>1644
                     /locus_tag="VHV99_21905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEX3638085.1"
                     /translation="MHTLPATDIELAAWLRLSLAPGLKPAALRLLLSAFGLPEAIFAQ
                     STEALAEIAGEAAARAVLAPAGPGFDAQLDAVIAWGALPGNHVVTLDDPAYPPALLTM
                     PDPPPLLYIKGRLDLLHTRAVA"
BASE COUNT          286 a          517 c          517 g          324 t
ORIGIN      
        1 gtgcgcgctt cggaaatcat cgcgccggta tcgagaccaa cgtccatctg catcagcgtg
       61 atgccggttt ccgtgtcgcc cgcctcgatc gcgcggtgga tcggcgcggc gccgcgccag
      121 cgcggcaaca acgacgcgtg aatgttgatg cagcccagcg gcggaatatc gagcacttcc
      181 tgcggcagga tcagaccgta ggcggccacg accatgacgt cgtgcggcgt ggcgcgcaac
      241 tggtcgatag cggctgcggc ttccaccgga tacttgccgg tgcggcgcaa cgaaggcggc
      301 tgcgccacgt caattccatg ttcctgcgca taacgcttga cgggactcgc ctgcaatttc
      361 atcccgcgcc cagcaggttg atcttgccgg gtcagcacga gcggcaccgg aaaaccggcg
      421 ccatgaatcg cggccagcgc ggccgcggcg aactccggcg taccggcaaa gataacgcgc
      481 aacgaatgac tcatgaacag ggacagtagg cgaggtggac gcgcattaca ttgcgtgggc
      541 gagcttcttc atcttgctct tgatgcgcgt ctgcttcagc gacgacaggt actcgacgaa
      601 cacgcggccc atcaggtgat ccatttcgtg ctggatgcaa accgcgagca agccttcgca
      661 atccagctcg aaggcttcgc ccttctggtt cagtgcacgc acgcgcactt tttccgcgcg
      721 ctcgacgttg tcgtagatac cgggcaccga gaggcagccc tcttccgaca gctttcgctc
      781 gtcgctcgac cagatgattt ccgggttgat gaaggcgagc agttcgtcgt gcgtttccga
      841 cacgtcgatc acgatcacgc gttcatgcac gtccacctgg gtcgccgcga ggccgacgcc
      901 gggcgcggcg tacattgttt cggccatgtc ggcgacaagg cggcgaatgc ggtcgttgac
      961 cacttcgacc ggcttcgcaa tcttgtgcag ccgcttgtcc gggtaattga ggatgttcag
     1021 taaagccatg atcttgagtg tcttttatag gcgccgctga ccggtggaag gctagcgttg
     1081 gccgtttggc caggttgtgg gcgcatcgcg ttacgcagag gatagatgct gtcgaaccgg
     1141 ttcgattcaa cgcgccacgt gcgctaacga cgagcccgcg cgggtgggcg cggcgctgca
     1201 gttatttcgg atgatgaaaa ttttagcatg gcgcctgcct gcccgctggc cctgtaggag
     1261 gatccactgt caatgcacac cttgcccgca accgatattg aactcgctgc ctggctgcgg
     1321 ctttcgctcg cgccggggct gaaacccgcc gccctgcgct tattgctgag cgcttttgga
     1381 ttgccggaag cgattttcgc tcagtcgacc gaagcacttg ccgagatcgc cggcgaggct
     1441 gcagcgcgcg ccgtgctggc acctgccggc ccaggcttcg atgcgcagct cgacgcggtg
     1501 attgcatggg gcgcgctgcc tggcaaccac gtggtgacgc tcgacgaccc cgcctacccg
     1561 cccgcactac tgacgatgcc cgatccgccg cccctactat atataaaggg tcggctggat
     1621 ctgttgcaca cgcgggcggt cgcg
//
LOCUS       DATASH010000816         6870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1919982,
            whole genome shotgun sequence.
ACCESSION   DATASH010000816 DATASH010000000
VERSION     DATASH010000816.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6870
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1919982"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="VHV99_21910"
     CDS             <1..869
                     /locus_tag="VHV99_21910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX3638086.1"
                     /translation="DTLLDDDATYAMRAAFASEPKLVAAAGILVPVCGKSVSGRVFQW
                     FQTYEYMRNFIARFAWMRADSLLLISGAFASFKRDALVDVGGFDPQCLVEDYELIHRL
                     RRYSVDHGLGWDVRVVGDAHARTDAPDNLGSFLRQRRRWFAGFLQTQYWNRDMTGNRR
                     YGTLGMLMLPVKAIDTMQPIYGLTAFALLLGFLIGGHGAIVVSIFSVIFLKTAIDLAF
                     YLWCIHLYRRWTGERTGSSLWMAVLAAIAEPFTFQLVRHVGALLGWLHFLRGGRSWGV
                     QRRSGLVTHDES"
     gene            993..2231
                     /locus_tag="VHV99_21915"
     CDS             993..2231
                     /locus_tag="VHV99_21915"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX3638087.1"
                     /translation="MHAVPPLEQDTVDAPVDATAAHAATQANAPATGNVHTPDKDANG
                     APVRDLRRVGIHPDYWYPLAWSHEVKRGKTHGVTFAGEPIVLARTESGKVFALEDRCA
                     HRQVPLHQGVVDGESIRCGYHGWTYDCSGTCIDVPYLGRDRLPNGVRSYPCREVEGLI
                     FVFPGNAGLAEERPLPAFGAVSDKKYKTRRFGRPVNCHYSFMHENLMDMNHQFLHRRQ
                     MGQMRARSLGRRRGEGWVEVDYTFARMAGQQPIGEAIVFGQSRKTGGDNDKDVMTIRT
                     EYPYQTLQIRTSDQTLVMNLWICYVPLDREQRTNRTFGLLSIRKPGIPGVLNLAWPLL
                     VWFTERIFKEDREIVEAEQRAHDSQGADWNHEVFPVINELRSLLRESGAPDHVVGAGT
                     GGAAVIRFWDSRHGNPLSNT"
     gene            2713..3129
                     /locus_tag="VHV99_21920"
     CDS             2713..3129
                     /locus_tag="VHV99_21920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEX3638088.1"
                     /translation="MYKKGVAVEIQFSPERLNDGAGDPYWIDLTRAEAQRLLESLQVR
                     LAESGVGTVAPMVVSLDDTPALQAGAAPHAAAESPASADDFKQWVCVICGWIYDEAAG
                     LPEEGIAPGTRWADVPVDWRCPLCDVGKEDFALVEF"
     gene            complement(3126..3971)
                     /locus_tag="VHV99_21925"
     CDS             complement(3126..3971)
                     /locus_tag="VHV99_21925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEX3638089.1"
                     /translation="MMNRDNPVWLITGCSTGFGRELAKLVLERGWRAVVTARDPSKVK
                     DIAEGHGDRALVLPLDVTNRTQIEDVVTQAKQRFGRVDALVNNAGYGYLAAIEEGEDD
                     EVRAMFETNVFGLVDLTKAVLPIMREQHSGLIVNVSSIGGITSFAATGYYHATKYAVE
                     GLSESLATEVKPLGIDVLIVEPGPFRTNWAGPSIKQSATVIDAYAATAGERRKQTEAR
                     SGKQAGDPVRAAQAIIDAALSDNPPLRLLLGKTALELARKKLDFMRGDFDGWEATTIG
                     ADFPA"
     gene            complement(4050..4259)
                     /locus_tag="VHV99_21930"
     CDS             complement(4050..4259)
                     /locus_tag="VHV99_21930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638090.1"
                     /translation="MAYGMQRGVERRRPASRAAASPSTADMRKRTPEAIRRSHPLAGA
                     FVEALQSEAAALRLQLNVRTKRTQP"
     gene            complement(4287..5207)
                     /locus_tag="VHV99_21935"
     CDS             complement(4287..5207)
                     /locus_tag="VHV99_21935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX3638091.1"
                     /translation="MAMTISQLRAFTAVAEHGSIRAASRALGIAQSGITQQLQNLESI
                     LGATLFTRTNRGIALTALGQRLLQRASAILSECERAEQDVQQLGGDYAGEVTFGMTTE
                     PLIDAFAPVLTEFRARFERVAVHLRTGTSRMMISWIREGTLDFAVALVSKHTDTADLS
                     VTPFYPSDPVIVCRRGHPKAGATSLAELVDCTWVATPSPNLTGDPQVNRLNHLFESHG
                     LPPPRIIATVEGLYETLHLVGATDCLSLEASVVVKRGPFASTLTSINVHERAVEQNVC
                     LLQRAAIPVTPAAQELATMIASYTRTVRAQ"
     gene            5334..6101
                     /locus_tag="VHV99_21940"
     CDS             5334..6101
                     /locus_tag="VHV99_21940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018423936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX3638092.1"
                     /translation="MKIPLMILSAALAFSSGAFAADSATLRLGIDPSYPPMDAKAPDG
                     SFKGFDVDLGNEICRRIHARCQWVELEFSGMIPALQARKIDAILSSMAITEKREQQIL
                     FSSKLFQFKSRLIARQGSPLAAGTNALAGKQIGVQSGTQFEGYALKNWAPLGVHVVAY
                     KSQDEVFADLQNGRLDGALLGSVEADYGFLRTPAGKGFAFVGEPLSMGDRGVGIGLRK
                     DETAVQASINAAIASMLKDGTYAQIAKKYFDFDPYGN"
     gene            6141..>6870
                     /locus_tag="VHV99_21945"
     CDS             6141..>6870
                     /locus_tag="VHV99_21945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HEX3638093.1"
                     /translation="MNKQSIPLLSPAIGTHRELVSFHFGPADSGQKIYIQSSLHADET
                     PAMLTTVLLKRRLLELEKQGALNAEIVLVPVANPVGLGQYVLGQFVGRFDLGSGKNFN
                     RHFMQFSKLVARAKEVLGSDAAENRRIVRELIAAELAEQKPLTEFESLQLALLKLSFD
                     ADVVIDLHCSLEAAMHLYTSEAAWPEFEPLSRYLGAQASLLATNSGGESFDETHSLLW
                     WTLRQEMPVDKPVPNGTIAVTVECR"
BASE COUNT         1195 a         2022 c         2219 g         1434 t
ORIGIN      
        1 ccgacacgct gctcgacgac gacgccacct acgcgatgcg cgccgctttc gcgagcgagc
       61 cgaagctggt cgcggcggcc ggcattctgg tgccggtgtg cggcaaatcg gtttcgggcc
      121 gcgtgttcca gtggttccag acttacgaat acatgcgcaa cttcatcgcc cgttttgcgt
      181 ggatgcgcgc cgacagtctg ctgctgattt caggcgcgtt cgcgtcgttc aagcgcgacg
      241 cgttggtcga cgtgggcggc ttcgatccgc agtgcctggt cgaagactat gagctgattc
      301 accggctgcg ccgctattcg gtcgaccacg gtctcggctg ggacgtgcgc gtggtcggcg
      361 atgcccatgc gcgtaccgac gcgccggaca acctcggcag ttttctgcgg cagcgccgcc
      421 gctggttcgc cggcttcctg caaacgcagt actggaaccg cgacatgacc ggcaaccggc
      481 gctacggcac gctgggcatg ctgatgctgc cggtcaaggc catcgatacg atgcaaccga
      541 tctacgggct gaccgccttc gcattgctgc tcggttttct gattggcggg catggcgcga
      601 tcgtggtgtc gattttcagc gtgatcttcc tcaaaacggc gatcgacctc gcgttttatc
      661 tctggtgtat ccatctgtac cgccgctgga ccggcgagcg caccggctcc agcctgtgga
      721 tggcggtgct cgcggcaatc gccgagccgt tcacgttcca gttggtgcgg catgtcgggg
      781 cactgctcgg ctggctgcat ttcctgcgtg gcggacgctc gtggggcgtg caacggcgct
      841 ccggcctcgt gacgcacgac gaaagttagc gggcggtctc cggtagtcgg cgaatccata
      901 ccacttcggc ttcgagtaga ctgtacgacg atgtcggccc gcgaattgca ggccgatcac
      961 cttctatccg ctcgtgcacg gaggctccac gtatgcatgc tgttcccccg ctcgaacaag
     1021 acaccgtcga tgcgccggtc gatgcgacgg ctgctcatgc agccactcag gcaaacgcac
     1081 ctgccacagg gaatgtccac acgccggata aagacgccaa cggcgccccg gtgcgcgatc
     1141 tgcgccgtgt agggattcac cctgattact ggtatccgct cgcgtggtcg catgaagtga
     1201 aacggggcaa gacgcatggt gtgacgttcg cgggagagcc gatcgtgctg gcgcgcacgg
     1261 agtcgggcaa ggtgttcgcg ctcgaagatc gttgcgcgca tcgtcaggtg cccttgcatc
     1321 agggcgtggt ggacggcgaa tcgatccgct gcggctatca cggctggacc tacgactgct
     1381 ccggcacgtg catcgacgtg ccgtatctgg gccgcgatcg tctgccgaac ggcgtgcgtt
     1441 cttatccgtg ccgcgaagtc gaagggctga ttttcgtgtt tcccggcaat gccgggttgg
     1501 ccgaagaacg gccgctaccc gcgttcggcg ccgtatccga caagaagtac aagacgcgcc
     1561 gtttcggccg tccggtgaat tgccactatt cgttcatgca cgagaacctg atggatatga
     1621 accatcagtt tctgcatcgc cggcagatgg gtcagatgcg cgcgcgctcg ctggggcggc
     1681 ggcgcggtga aggctgggtc gaagtcgatt acacgtttgc gcgcatggcc ggccagcagc
     1741 cgatcggcga agcgatcgtg ttcggccaga gccgcaagac cggcggcgac aacgacaagg
     1801 acgtgatgac gatccgcacc gagtatccgt atcagacgct gcagattcgc acgtccgacc
     1861 agacgctggt gatgaattta tggatctgct acgtgccgct cgatcgcgag cagcgcacca
     1921 accgcacctt tggcctgctg tcgattcgca aaccgggtat tcccggcgtg ctgaatctcg
     1981 cgtggccact gctcgtgtgg ttcaccgaac gcattttcaa ggaagaccgc gagatcgtcg
     2041 aagccgaaca gcgtgcgcac gattcgcaag gcgcggactg gaatcacgag gtgtttccgg
     2101 tcatcaacga actacgcagc ttgctgcgcg agagcggtgc gccggatcat gtggtgggcg
     2161 ccggcaccgg cggtgccgcg gtgatccgct tctgggattc gcgtcacggc aatcccttgt
     2221 cgaatacctg atatcgggtt ttttggcgcc acgcgggacc gtaggatcat tggcgacgat
     2281 gagcgagagc cggcacagca attgtggcgg cggtgtctga gcgtgccggg gaggtggctt
     2341 ttcgtaagtt ggctttcccc ccggtggtga atgagtccat ctccgtgcgg gagggaatcc
     2401 aggccgttca agcagcgata atggcatctc acacgccagg atttgcgcca atcgagatac
     2461 gccggcctgc tacccgtgta ccatttcgct tttttcgatc tttaagaaca cctcgtgact
     2521 acgcaacaca tccctaccgc atcccgtaag tccctcactc tgctgcaact gcttggcctc
     2581 attggcgccg ctggactgat cgccgccatc gtgctgaacc agttcgtctg atccccgcca
     2641 cgaagccctc gcttcaagca tccgacccta gaccggcgct cgccgccggc ttcatcgata
     2701 ttccgatctg ccatgtacaa gaagggcgta gccgtagaaa tccagttttc gcctgagcgg
     2761 ctcaatgacg gcgccggcga cccgtactgg attgatttga cgcgggccga agcgcaacgg
     2821 ctgttggaaa gcttgcaggt acgcctcgct gaaagcggtg tggggacggt cgcgccgatg
     2881 gtggtgagcc tcgatgacac gccggcattg caagctggcg ctgcaccgca tgcggcggca
     2941 gaaagcccgg catccgcgga cgacttcaaa caatgggtct gcgtgatctg cggctggatc
     3001 tacgatgaag ccgccggctt gccggaagaa ggtatcgcgc cgggcacccg ctgggcggat
     3061 gtcccggtcg actggcgttg cccactatgc gacgtgggca aggaagattt tgcgctggtt
     3121 gagttttaag ccgggaaatc cgcgccgatc gtggtggctt cccagccatc gaagtcgccg
     3181 cgcatgaagt ccagcttctt gcgcgccagt tcgagcgcgg ttttgccgag cagcaagcgc
     3241 agtggcgggt tgtccgacag cgcggcgtcg atgatggctt gcgcggcgcg taccgggtcg
     3301 cctgcctgtt tgccgctgcg cgcttcggtc tgcttgcggc gctcgccggc cgtggccgca
     3361 taggcatcga tcacggttgc cgactgcttg atcgacggcc ctgcccagtt cgtgcggaac
     3421 ggacccggct cgacgatcaa gacgtcgatg ccgagcggct tcacctccgt cgcgagcgat
     3481 tcggacaagc cttccactgc gtacttggtg gcatggtagt agccggtcgc ggcgaaactc
     3541 gtaatgccgc caatcgacga cacgttcacg atcagcccgc tgtgctgctc gcgcatgatc
     3601 ggcagcacgg ccttcgtcaa gtcgacgaga ccgaacacgt tggtctcgaa catcgcgcgg
     3661 acttcgtcgt cttcgccttc ttcgatcgcg gccagatagc cgtaacccgc gttattgacg
     3721 agcgcgtcga cgcggccgaa acgctgtttg gcttgcgtga ccacgtcttc gatctgcgtg
     3781 cggttcgtca cgtcgagcgg cagaaccagt gcgcgatcgc cgtgtccttc ggcgatatct
     3841 tttaccttcg acgggtcgcg ggcggtgact accgcacgcc agccgcgctc cagcaccagc
     3901 ttcgccaatt cgcggccgaa gccggtggaa cagcctgtaa tcagccagac ggggttgtct
     3961 cgattcatca tttgggaaac tcctgttgag gggtcaaacg gcgtgtgaaa tgccgcgcgg
     4021 cgcagagatg cagggtatct gcaggtgcgt tatggttgcg tgcgtttggt gcgaacgttt
     4081 aattgtagcc gtagcgcggc agcttcgctt tgcagcgctt cgacgaacgc accggccagc
     4141 ggatggctcc tacggatggc ctccggcgtg cgcttgcgca tgtcagctgt gctgggcgaa
     4201 gcggcggcgc ggcttgcagg tcgtcgacgt tcaacgccac gctgcatgcc gtaggccatc
     4261 gctcgccttg ctgcatcgac ctggcctcat tgcgccctta ccgtgcgcgt gtacgacgca
     4321 atcatcgtcg cgagctcctg cgcggcaggg gtgaccggaa tcgccgcgcg ttgcaacaga
     4381 cagacgttct gttcaaccgc tcgttcgtgc acattgattg acgtcagtgt gctcgcaaac
     4441 ggcccacgct tcacgacgac cgacgcctcc agcgacaggc aatccgtcgc gccaaccaaa
     4501 tgcagcgtct cgtacagacc ctccaccgtc gcgatgattc tgggcggcgg caagccgtgg
     4561 ctttcgaaca gatggttcag acggttaacc tgcggatcgc cggtgaggtt cggcgagggc
     4621 gtggccaccc acgtgcaatc gacgagttcg gcaagcgacg tcgcccccgc tttcggatga
     4681 ccgcgacggc agacgatcac cggatcggac gggtagaacg gcgtgacgga cagatcggcc
     4741 gtgtcggtgt gcttcgatac cagcgcgaca gcgaaatcga gcgtgccttc gcgaatccag
     4801 gaaatcatca tccgcgaagt gccggtgcgc aaatgcaccg cgacccgctc gaagcgcgcg
     4861 cggaactccg tcaacaccgg cgcgaacgcg tcgatgagcg gctcggtggt catgccgaac
     4921 gtgacctcgc ccgcgtagtc gccgcccaat tgctgcacat cctgctcggc gcgctcgcat
     4981 tcgctgagaa tcgcgctggc gcgctgcaac agccgctggc cgagcgcggt cagcgcgatg
     5041 ccgcggttcg tgcgagtgaa taaggtcgcg cccaggatcg attcgaggtt ctgcaactgc
     5101 tgcgtgatgc cgctctgcgc gatgccaagc gcccgcgacg ctgcgcgaat gctgccgtgc
     5161 tcggcgacag cagtgaacgc gcgcaactgg gagatcgtca tggccatgag ggagccggtg
     5221 atcggtaaaa gtgatcatga tattactttc gggacttctt tgaggcgcgg cggcggctta
     5281 ggatgcccct gtgttaatcc cggtagtagt ccgttcaccc acacccgtct cccatgaaaa
     5341 ttccgctgat gattttgagc gccgcgctgg ctttcagcag tggtgcgttt gccgccgact
     5401 ccgccacgct gcgcctcggt atcgatccga gctatccgcc gatggacgcc aaggcgcccg
     5461 acggcagctt caaaggtttc gacgtcgatc tcggcaacga aatctgccgg cgcatccatg
     5521 cgcgttgcca gtgggtcgaa ctcgagttct ccgggatgat cccggcgctc caggcgcgca
     5581 agatcgacgc gatcctttcg tcgatggcga tcacggagaa acgcgagcag cagattctgt
     5641 tttcgtcgaa gctgttccag ttcaaatcac ggctgatcgc caggcaaggt tcgccgctcg
     5701 cagccgggac gaatgcgttg gccggcaagc agatcggtgt gcagtcgggg acgcagttcg
     5761 aaggctatgc gttgaagaac tgggcgccgc ttggcgtgca tgtggtcgcg tacaagagtc
     5821 aggatgaggt gtttgccgac ttgcagaacg gccgtcttga tggcgcgttg ctcggttcgg
     5881 tcgaagccga ctacggcttt ttgcgcacgc cggcggggaa gggatttgcg tttgtcggcg
     5941 agccgctttc gatgggtgat cgtggcgtcg gcatcggttt gcgtaaggat gagactgcgg
     6001 tgcaggcgtc tattaacgcg gcgattgctt cgatgctgaa agacggtacg tatgcgcaga
     6061 tcgcgaagaa gtacttcgac ttcgatccgt acggcaattg atttttcgtt ttacttctct
     6121 tctacagacc tctggctccc atgaacaagc aatcgattcc gcttctatcg ccggctatcg
     6181 gcacgcaccg cgaactggtg tcgtttcatt ttggtcccgc ggatagcggg cagaagattt
     6241 atatccagtc gtcgctgcat gccgatgaaa cgccggcgat gctgacgacg gttttgctta
     6301 agcgtcgact gctcgaactg gagaagcagg gtgcgttgaa tgccgagatt gtgctggtgc
     6361 cggtggcgaa tccggttgga ctggggcagt acgtgcttgg gcagtttgtt gggcgattcg
     6421 atctgggcag tggcaagaac ttcaatcggc atttcatgca gttttcgaag ctggtggcgc
     6481 gggctaagga ggttttgggt tcggatgctg cggagaatcg gcggattgtt cgggagttga
     6541 ttgctgctga actggctgaa cagaagccgt tgactgagtt tgagtctttg cagttggcat
     6601 tgctcaagct ctcattcgat gcggatgtag tgatcgattt gcattgctct ttggaagcgg
     6661 cgatgcatct gtataccagt gaggctgctt ggcctgagtt cgagcctctg tcacggtatc
     6721 tgggggcgca ggcttcgctg ctggctacga actcgggcgg ggagtcgttt gatgagacgc
     6781 atagcttgtt gtggtggacg ctgcggcagg aaatgcctgt tgataagccc gtgcccaatg
     6841 ggacgattgc tgtgacggtg gagtgtcgtg
//
LOCUS       DATASH010000817         5517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6863116,
            whole genome shotgun sequence.
ACCESSION   DATASH010000817 DATASH010000000
VERSION     DATASH010000817.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5517
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6863116"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..975
                     /locus_tag="VHV99_21950"
     CDS             <1..975
                     /locus_tag="VHV99_21950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX3638094.1"
                     /translation="AMQPLMPLWKDRQLAIVQGVSYPQPNLSHFRSIEIWDTGSRADQ
                     YLREGWLTRAFAQAPVPAGFAADGLVIGSAEMGPLANGARAIALFNPAQFVKAARLAT
                     PVSLHERNPELAHILDVENDIVKAADHLRPMQGEAQLKTVFPGGAFGSSIRAAMQALA
                     ASDAPKQVSLADTLQGQPKSGQGVAVIRLTLNGFDTHQNQPGQQAALLKQLAEGFASM
                     KSALTELGRWDETLVMTYGEFGRRPRENQSNGTDHGTVAAHFVMGGRVLGGLYGLPPA
                     LARLDGNGNLPVGVDFRQLYATVLGPWWGLEASTILQQRFEPLSLLRV"
     gene            complement(1556..2380)
                     /locus_tag="VHV99_21955"
     CDS             complement(1556..2380)
                     /locus_tag="VHV99_21955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018432023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HEX3638095.1"
                     /translation="MNFDEYCQQKAAPPGSSTYYALRQAPAASQPLLAALFALRREFE
                     ETVKETSDPAIGRTKLAWWQNEIAALASGSPSHPVSKALAAYLPDVQSEYPALQALLA
                     GFEMDLDQARYLDYPNLRRYVQGVGGTFASLVARATAKDGAQAPSWSAPLGEALQLAQ
                     FVVETGNDARHGRIYIPIDEMQRFKVTAADLINRKYTDAFTELMRFETKRARDALHAA
                     LAAIPANERRSQRTLVAQAALALALLDEIERDGYHVLHQRIALTPIRKLWIAWRAR"
     gene            2601..2687
                     /locus_tag="VHV99_21960"
     tRNA            2601..2687
                     /locus_tag="VHV99_21960"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2635..2637,aa:Leu,seq:tag)
     gene            complement(2783..3076)
                     /locus_tag="VHV99_21965"
     CDS             complement(2783..3076)
                     /locus_tag="VHV99_21965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008924403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638096.1"
                     /translation="MDINKQVAALTASELQTAGASQATAIAVSVLLRHLRSPELVKLL
                     SSAFENHQAVMLQTPWPDQMLQAFESTRRFLEGAAQAELPGTQADQQSPAPGA"
     gene            complement(3741..4193)
                     /locus_tag="VHV99_21970"
     CDS             complement(3741..4193)
                     /locus_tag="VHV99_21970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX3638097.1"
                     /translation="MKASAKAGLEQLLTYRLHVLNKLAERGIGERYQDKLGVTLPEAR
                     VIASVGSFGPFSIMELARHANLDKSQASRAAEALIKQGLVKRDPSAEDGRVVLVSLTP
                     EGRALYRKVMPIARKWNGDMFHCLDEQEKLAFGQALDKIINAMAEREE"
     gene            complement(4257..5402)
                     /locus_tag="VHV99_21975"
     CDS             complement(4257..5402)
                     /locus_tag="VHV99_21975"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEX3638098.1"
                     /translation="MPNSPSSPVVVIAPDSFKGSLSAEQVAQAIAAGILRARLDAEVR
                     ICPMADGGEGTLDAMLTSGGERRKLSVRGAAGPVREALTGLLADGSAIIETAEIVGIT
                     DPVGMGVPVEARSTRGMGEAIRALLDVGVRRFFVALGGSSTNDGGAGLLAGLGLKLFD
                     AQDKELDATPEQLARLARLDVSQLDARLADTQFVGMSDVDNPLTGDHGATAVFGPQKG
                     VKPEQIAAIDTVLGRFADLLEAALGHTARDQPGAGAAGGLGFALHMLGAQFEPGAETV
                     ARQIGLDAALEGATWLITGEGRSDVQTLHGKAPFIACRHAQAVGVPATLLSGAVDSAA
                     LPRLAEHFSGCFSPAPGPITLEVAIRDAARLLADEAEQLTRLKYGAR"
BASE COUNT          979 a         1654 c         1807 g         1077 t
ORIGIN      
        1 gccatgcagc cattgatgcc gctctggaaa gaccggcaac tcgcgatcgt gcagggcgtc
       61 agttatccgc aaccgaacct ctcgcatttt cgatccatcg agatttggga caccggttcg
      121 cgcgccgatc aatatctgcg cgaaggctgg ctcacgcggg cattcgcgca ggcgccggtg
      181 cccgccggtt ttgccgccga cggcctggtg atcggcagcg cggaaatggg accgcttgcc
      241 aacggcgcac gtgcgatcgc gctgttcaat ccggcgcagt tcgtcaaggc tgcgcgtctc
      301 gcgacaccag tgtcgctgca cgagcgtaat ccggaattgg cgcacattct cgacgtcgaa
      361 aacgacatcg tgaaagccgc cgaccatctg cgtcccatgc aaggtgaggc gcaattgaag
      421 acggtgttcc ccggtggggc attcggcagt tcgatcaggg ccgcgatgca agcgctcgcc
      481 gccagcgacg ccccgaagca agtctcactg gcggacacgc tccagggaca gccgaagagc
      541 gggcagggcg tcgcggtgat ccgtttgacg ttgaacggtt tcgatacgca ccagaaccag
      601 cccggtcagc aggcggcgct gctcaagcaa ctggccgaag ggtttgcatc gatgaagtcg
      661 gcgctgacgg aactcggccg ttgggacgag acgctggtga tgacctatgg cgaattcggc
      721 aggcgcccgc gggagaatca gagcaacggc accgaccacg gcaccgtggc ggcgcatttc
      781 gtgatgggcg ggcgcgtgct gggcgggttg tatggcttgc cgccggcgct tgcccggctc
      841 gacggcaatg gcaatttgcc ggtcggcgtc gatttccggc aactctatgc gaccgtgctc
      901 gggccgtggt ggggactcga ggcgtccacg atccttcagc agcggttcga gccgctgtcg
      961 ttgctacggg tctgatagtt ggcggtgctt gcgcgtcagc ttgaggtggc ggtcaactcg
     1021 acgcgtccgt gatgtgaaac agcgattcga gccgctgccg ttgctgcggg cctgataatt
     1081 agcggggttt gcacatcagt ttgaggtggc ggtcaactcg acgcgtccgt gatctgaaac
     1141 agcgattaga ggcgctgccg ttgctacggg tctgataatt agtggtggtt gcacatcagc
     1201 ttgaggtggc ggtcaactcg acgcgtccgt gatgtgaaac agcgattaga ggcgctgcca
     1261 ttgctacggg tctgataatt agcggtgctt gcgcgtcagc ttgaggtggc ggtcaactcg
     1321 acgcgtccgt gatgtgaaac agcgattaga ggcgctgcca ttgctacggg tctgataatt
     1381 agcggggttt gcacattagc ttgaggtggg cagccaactt gacgcgtacg atcttggagg
     1441 ggcggttcga gccgacgtcg ttgctagcag gtatctaata gttggtggca cttgcgcctc
     1501 agcatgaagt ggcggccatt gtgtcgcccg cgcccgctta ggccacttat cccgcttacc
     1561 tcgcgcgcca cgcgatccaa agtttgcgga tcggcgtcaa cgcgatgcgc tgatgcagca
     1621 cgtgatagcc gtcgcgttcg atttcatcga gcagcgccaa ggcgagcgcc gcctgcgcca
     1681 ccaaagtgcg ctgcgagcgc cgctcgttcg cggggatcgc ggcgagcgcg gcatgcagcg
     1741 catcgcgtgc ccgtttggtt tcgaaacgca tcagctctgt aaacgcatcg gtatatttcc
     1801 gattgatcag atccgccgcg gtgaccttaa agcgctgcat ttcgtcgatc ggaatataga
     1861 tgcggccgtg ccgcgcatca ttgccggttt cgacgacgaa ctgcgcgagc tgcaacgcct
     1921 caccgagcgg cgcggaccag ctcggcgcct gtgcgccgtc tttcgccgtg gcgcgcgcga
     1981 ccagcgacgc gaaggtcccg ccgacgcctt gcacgtaacg ccgcagattc gggtagtcga
     2041 ggtagcgcgc ctgatcgagg tccatttcga agccggcgag caatgcctgt aacgccggat
     2101 actcgctctg cacgtcgggc agataagccg ccagcgcctt tgaaaccgga tgcgacggcg
     2161 agcccgacgc gagcgcggcg atttcgtttt gccaccacgc gagtttggtg cggccgatgg
     2221 ccggatcgct ggtttccttg acggtttcct cgaactcgcg gcgcagcgcg aacagcgcgg
     2281 ccaggagcgg ttggcttgcc gcgggcgcct ggcgaagcgc atagtaagtg ctcgatccgg
     2341 gcggcgccgc tttctgctgg caatattcgt cgaaattcac gggattggaa tgtgggggaa
     2401 gggcaggcga agagggcacg gcgcgcgtgc aattagccaa aaattgtagc atcgacgtcc
     2461 ctcgggcaga tgcatgcgca aatgcagaca agcgcgaatc aatcggttag aatctcgcgc
     2521 ttacgctttc gggcgcggtt tttttacgcg atcgtgagcc tgcaggacaa cgggcagaac
     2581 gcacagcgtt ttgcccggct gcgagggtgg cgaaattggt agacgcacca ggtttaggtc
     2641 ctgacgcccg caagggtgta ggggttcgag tcccctccct cgcaccattc cgatgtagca
     2701 gagaaccccg ttaagtcttt ggactgacgg ggttttttgt ttttcaagtg atttgcgtcg
     2761 ggccgcgcga atccggccgt gctcaggcgc ccggcgctgg cgattgttga tccgcttgtg
     2821 tgcctggcaa ctcggcttgc gcggcgcctt ccagaaaccg gcgggtcgat tcgaacgcct
     2881 gcagcatctg atcgggccac ggtgtttgca gcatgaccgc ctgatggttc tcgaatgccg
     2941 acgacaacag ttttaccagt tcaggcgagc gcagatgacg cagcagcaca ctgacggcga
     3001 tcgcggttgc ctggctcgcg ccggcggttt gaagttctga ggcggtgagg gccgctacct
     3061 gtttgttgat gtccactgat acttccttgg tgaaatgtgg gagattgaag gctcggtggc
     3121 caatcccgaa ttttgacacg gttgacgggt tgtcgcgaac gctgctgcct cgcgcagccg
     3181 cgtggactca aagcgctgtg atggttttca gcgcagttaa tccgttaagc gatatgccgg
     3241 caaaaaacca gcgtgcatcg ccggatctac gcaggcattc gacccgtccg ctcgatgcat
     3301 caaaagtgtg atttacctga tattctgcgc gccgcttttc gctgtactac tgatgcaata
     3361 ctcgcggatt aatccgtgcg actaaacgga tctcgctaca gaagacacta aagagggatg
     3421 cggtgggggc ggccttgccg ctgatgatcg tgatctgctg gcgctcgtgc cggtcctcgc
     3481 tctattgacg ttgagcctta tgcttcaagg cacaggaaag agctggcttc ggccgcagga
     3541 gaatcccggc ggaacggatt gcacggaacc taaaaatttg gacgtgccga gagattgatt
     3601 tgcattgcgg gaatgtgctc ccgtgggcat gactggcgca atgacgggcg gggccgaatc
     3661 gcaggatgcg gtccgcagcg cggatcgaag caagcaatcg cgcagcgcgg aagatgcgcc
     3721 ccgtgccggc gggccgttgc ttattcctcg cgctccgcca ttgcgttgat gatcttgtcg
     3781 agcgcctggc cgaacgcgag cttttcctgt tcgtcgaggc agtggaacat atccccgttc
     3841 cacttacggg cgatcggcat caccttgcga tagagcgcgc ggccctccgg ggtgagcgat
     3901 accagcacga cgcgcccgtc ttcggcgctc ggatcgcgtt tcacgagtcc ttgcttgatc
     3961 aatgcctcgg ccgcacggct ggcctgactt ttgtccaggt tggcatgccg cgccaattcc
     4021 atgatcgaaa acggtccgaa cgagcccact gacgcaatta cccgcgcctc aggcaacgtc
     4081 acgccgagct tgtcctgata gcgttcgccg atgccacgct cggcgagttt gttcagcaca
     4141 tgcagacggt acgtcaggag ctgctcgagt cctgctttgg cggaggcctt catggtcttc
     4201 ccgtgatgag tgaagcggtc gtggcgctgt gattgttgcg gcaaccgtcg cgccggtcaa
     4261 cgcgcgccgt atttcaggcg cgtcaattgt tcggcctcgt ccgccagcaa acgcgccgcg
     4321 tcgcgaatcg ccacttcgag cgtgatcggc ccgggggccg gcgaaaagca gccactgaag
     4381 tgttcggcga ggcgcggcag cgccgctgaa tcgaccgcgc ccgacagcag cgtggctggc
     4441 acgcccacag cctgcgcatg acggcaagcg atgaacggcg ctttgccgtg tagtgtttgc
     4501 acgtccgagc ggccttcgcc ggtgatcagc caggtcgcgc cttcaagcgc tgcgtccaga
     4561 ccgatctgac gcgcgacggt ttccgcaccc ggctcgaact gcgcgcccag catatgcagc
     4621 gcaaagccga gaccaccggc cgcacccgcg ccgggttggt cgcgtgccgt gtgcccgagc
     4681 gccgcctcca acagatcggc gaagcggccg aggacggtat cgatagcggc aatctgttcc
     4741 ggcttcacac ccttttgcgg gccgaacacc gcggtcgcgc cgtgatcccc ggtgagtggg
     4801 ttatccacat ccgacatgcc gacgaactgg gtgtccgcga ggcgggcgtc cagctgcgac
     4861 acgtcgagac gcgcgagccg ggcaagctgt tcgggcgtcg cgtcgagttc cttgtcttgc
     4921 gcgtcgaaaa gtttcaggcc gaggccggcc agcaagccgg cgccaccgtc gttcgtgctg
     4981 ctgccgccga gcgcgacgaa aaagcgccgt acgccgacgt cgagcaatgc gcgaatcgct
     5041 tcgcccatgc cacgcgtgct gcgcgcctcg accggcaccc ccatgccgac cggatccgtg
     5101 ataccgacga tttccgccgt ttcgatgatc gcgctgccgt ctgcaagcag gccggtgagt
     5161 gcctcccgca ccggaccagc cgcgccgcgc acgctcagct tgcggcgctc gccgccgctc
     5221 gtgagcatcg cgtcgagcgt gccttcgccg ccgtcggcca tcgggcagat gcgcacctcg
     5281 gcatccagcc gggcgcgcag gatgccggcc gcaatcgcct gcgcgacctg ttcggcgctg
     5341 agcgaacctt tgaaggaatc gggagcaatg acgacgacgg gcgaggacgg cgaattcggc
     5401 atggtgtctc tgaatggttg aagtcgttac gaggacgggt gacatgaaca gatgcggcgc
     5461 agccgcgagc acgtccgtta gcttatcagc atgggcgcgc cgactgaata tgtcgaa
//
LOCUS       DATASH010000818         3756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_7061822,
            whole genome shotgun sequence.
ACCESSION   DATASH010000818 DATASH010000000
VERSION     DATASH010000818.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7061822"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VHV99_21980"
     CDS             <1..204
                     /locus_tag="VHV99_21980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638099.1"
                     /translation="GKSGQHQRAGQIGQLPVAPEHRRRNKPEHIGPQPDVFHSHDIRH
                     ALDVSGLRGTQPPQQGSSALSKK"
     gene            complement(569..3427)
                     /gene="treY"
                     /locus_tag="VHV99_21985"
     CDS             complement(569..3427)
                     /gene="treY"
                     /locus_tag="VHV99_21985"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HEX3638100.1"
                     /translation="MTVPRSTLRLQFHRGFTFDDAAKHVDYFATLGISHVYASPITTA
                     EPGSMHGYDTVDYTQVNAECGGEAGLKRLVEKLRAHNMGLIVDMVPNHMGVGGSSNAW
                     WLDILEWGRHSTYARHFDVDWHSPDPALRGKVLVPTLGAPYGEELAASRIALHFAADS
                     GRFHIGYGPHVFPVCPTDYASILQSADRPDLNALAERFQGLTTQPADQPRAAEGRDML
                     CEFVAQSGASAIELVLQAYAPEDPVTRDRLHRLIERQHFRLAWWRTASDEVNWRRFFD
                     ISTLAGVRVERPEVFEAVHALIFRLYQEGVVDGLRIDHVDGLAEPREYCQRLRQRLTE
                     LRDAAPYVVVEKILGRGEPLRDDWPVDGTTGYDFMSDVGALLHDPAGAEPLAQTWAEF
                     TGRSPRFTDEALVARRKILAENLSAELDRAARALHRIARDSLTTRDFTFTALRRVLTE
                     LVVHFPVYRIYPQNGLRSAADNVYFEQALAGARQTLSRADHVVLDRVNAWLGGSAEEV
                     PNARVSPSQQQSPNGAPPSHAGSARRTAQTLFSQLTAPAAAKAVEDTACYRYGRLLSR
                     NEVGSDPGEFALSVEQFHAANLERSQRFPHAMLATATHDHKRGEDVRARLAVLSEIAH
                     DWSTTLRAWSTLNAPHRRALDGKPISSVGQDTSYDWAPGPAAEAMLYQTLVGCWPPDL
                     KPDDEAGVKELAERVAQWQLKALREAKLQTNWLAPDEAYEAGCRDFLFDILAPQRRDG
                     FLRELSAFVTRIGRAGALNSLQQTVLRLASPGIPDLYQGTELWDFSLVDPDNRRPVDF
                     ARREAWLAQTPPSEFLASWHDGRVKLAVVQRVLALRAHLPELLSQGAYLPLQVRGAQA
                     SNVIAFARRHGNAWAVVVASRLAAGLLGEVGDVPMVDPAKWGDTALDMPADLTARALF
                     DWLSPAAPKVDEGGLLYLRDALGAMPIAVLVEDGVPRS"
     gene            complement(3424..>3756)
                     /locus_tag="VHV99_21990"
     CDS             complement(3424..>3756)
                     /locus_tag="VHV99_21990"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEX3638101.1"
                     /translation="AFQQAGVAAPDVEAPPPDQAPVDEALAFVAATPGPLVTFPLEDL
                     LALVEQPNLPGSIDEHPNWRRRVSVPIDELFEDDTFSDRLLAIDRARRSATVPASSSN
                     ASSEPDTP"
BASE COUNT          703 a         1249 c         1191 g          613 t
ORIGIN      
        1 ggaaaatccg gccagcacca gcgcgctggc cagatcgggc aactgcctgt agctccggaa
       61 caccggcggc gaaataaacc cgagcatatc ggtccccaac ccgacgtgtt ccactcccac
      121 gacatccgcc atgcgcttga cgtcagcgga ttgcgcggca cgcagccccc gcagcagggc
      181 tcgtcggcgc tcagcaagaa gtaatcgacc gcgtgggctt gcgggtccag cggaatcatc
      241 caacccgata tgtcgaccac ggtgccgtgc gatgggtcgg cacgcccggt ttggatagtt
      301 ggccagtcga tctgcatcgg cgcttttctc gggtgcgttt attggcgagc cgatattacc
      361 cgacttttat ccgatggcag cacgccgacc ctgcgctcag aaggcgtgag aaggagagga
      421 gagaggagcc ggagcagaag gagagaggag gcacggagag tgaagccagc tccaaggctt
      481 cggtcttcgg cgccatgaac aattgcgccg ctctgcccac cggcatgttg atcattcacg
      541 cggcaaagta gcggagcagt cggcgagatc aactgcgtgg cacgccgtcc tccaccagca
      601 ccgcgatagg catcgcgcct aacgcatcgc gcagatacag caagccgcct tcatcgacct
      661 tgggcgccgc cggactcaac cagtcgaaca gcgcccgcgc agtgaggtcc gcaggcatgt
      721 cgagcgctgt atcgccccat ttagccggat cgaccatcgg cacatcgccg acttcgccga
      781 gcaacccggc agcaagccgg ctcgccacca cgacagccca cgcgttccca tgccgccgtg
      841 caaacgcgat cacattcgac gcctgcgcac cgcgcacctg gagcggcaga tacgcccctt
      901 gactgagcaa ctccggtaaa tgcgcgcgca acgccagcac acgctgcacc accgcaagct
      961 tcacccgacc gtcatgccag ctcgccagaa actccgaagg cggcgtctgc gccagccacg
     1021 cctcgcgcct ggcaaaatca accggccggc gattgtctgg atcgaccaga ctgaagtccc
     1081 acaattcagt gccttgataa agatcgggaa tcccaggcga tgccagccgt aacaccgtct
     1141 gctgcaaact gttgagcgca cccgcgcggc caattcgcgt tacgaacgcg gacaactcgc
     1201 gcaggaaccc gtcgcgccgc tgcggcgcca ggatatcgaa caggaaatcg cggcagccgg
     1261 cctcatacgc ttcatccggg gcgagccagt tggtttgcag ctttgcttcc cgcagcgcct
     1321 tcaattgcca ttgcgcgacg cgctcggcca gttccttgac gcccgcttca tcgtccggct
     1381 ttagatccgg cggccagcag cccaccaggg tctgatacag catcgcctcg gcggcggggc
     1441 ccggcgccca atcgtagctc gtgtcctggc cgacgctgct gatcggcttg ccgtcgagcg
     1501 cacgccgatg cggcgcgttc aaggtcgacc acgcacgcaa cgtcgtgctc cagtcgtgag
     1561 caatctcgct caataccgcc agccgcgcgc gcacgtcttc accgcgcttg tgatcgtgtg
     1621 tggccgtggc gagcatcgcg tgaggaaaac gttgcgagcg ctcgagattg gccgcatgaa
     1681 attgctcgac cgacaaagca aattcgcccg ggtccgagcc gacttcattg cgcgacagca
     1741 aacgtccata gcgataacac gccgtatctt caaccgcctt cgcggcggcc ggcgcggtca
     1801 attgcgaaaa cagcgtctgc gcggtgcgcc gcgccgaacc cgcatgactg ggcggcgcgc
     1861 cattcgggct ctgctgttgc gatggactga ctcgcgcatt cggtacttct tcggcgctgc
     1921 cgccgagcca ggcgttcacc cggtcgagca ccacgtgatc cgcgcgcgac agcgtttgcc
     1981 gcgcgccggc cagcgcttgc tcgaaataca cgttgtcggc ggcgctgcgc agcccgttct
     2041 gcggatagat ccgatacacc ggaaaatgca cgaccagttc ggtcagcacg cgccgcagcg
     2101 cggtgaatgt gaaatcgcgt gtggtcaatg aatcgcgggc aatgcggtgc aacgcgcgcg
     2161 ccgcgcggtc cagttccgcc gagaggttct cggcgaggat cttgcgtcgc gcgaccaggg
     2221 cttcgtcggt gaagcgcggg ctgcggcccg tgaattcggc ccacgtctgc gcgagcggtt
     2281 cggcgccggc cggatcgtgc agcagcgcgc ccacgtcgct catgaaatcg tagccggtgg
     2341 tgccgtccac gggccagtcg tcgcgcagcg gctcgccgcg gccgagaatc ttttcgacga
     2401 ccacatacgg cgcggcgtcg cgcagttcgg tgaggcgctg gcgcagacgc tgacagtact
     2461 cgcgcggttc agcgagcccg tcgacgtggt cgatccgcaa accatccacc acgccttcct
     2521 gatacagacg gaaaatcagc gcgtggaccg cctcgaatac ttccggccgc tccacccgca
     2581 caccggccag cgtcgagatg tcgaagaaac gccgccagtt cacttcgtcc gaagccgtgc
     2641 gccaccacgc gagacggaag tgctgccgtt cgatcagccg atgcaaacgg tcacgcgtaa
     2701 cgggatcttc gggcgcatac gcttgcagca ccagctcgat cgctgaggcg ccgctttgcg
     2761 cgacgaattc acacagcatg tcgcgccctt cggcggcgcg cggctgatcc gccggctgcg
     2821 tcgtcaggcc ctggaaacgt tcagccagtg cgttgagatc ggggcggtcg gcgctttgca
     2881 aaatcgatgc gtaatcggtc ggacacaccg gaaacacatg cggcccatag ccgatgtgaa
     2941 agcgcccact gtccgccgca aaatgcagcg caatcctgct cgccgcaagt tcctcgccgt
     3001 acggcgcacc gagcgtcggc accagcacct tgccgcgcaa ggccggatcg ggcgaatgcc
     3061 agtcgacgtc gaaatggcgc gcgtacgtgc tatgccggcc ccattcgagg atatcgagcc
     3121 accacgcgtt gctcgagccg ccgacaccca tatggttcgg caccatgtcg acgatcagcc
     3181 ccatgttgtg cgcacgcagt ttttcgacga ggcgtttcag gccagcctcg ccgccgcatt
     3241 cggcattgac ctgggtgtag tcgacggtat cgtagccgtg catcgaaccc ggctcggccg
     3301 tggtgatggg cgacgcgtac acgtggctga tgccgagcgt ggcgaagtag tcgacatgct
     3361 tcgcggcatc gtcgaaggta aagcctcgat gaaattggag gcgaagcgtg gagcgcggga
     3421 cggtcatggc gtatcaggct ccgaagaagc gttggaagaa gaagcgggaa cggttgcgct
     3481 gcggcgcgcg cggtcgatgg ccagcaggcg gtcgctgaag gtgtcgtcct cgaacagttc
     3541 gtcgatgggc acactgacgc ggcggcgcca gttcggatgc tcgtcgatcg agccgggcag
     3601 attcggctgc tcgaccagtg cgagcaaatc ttcgagcggg aacgtgacga gcggcccagg
     3661 cgtggcggcg acgaaggcga gcgcttcatc cacgggcgcc tggtcgggcg gcggcgcttc
     3721 cacgtcgggc gcggcgacgc cggcttgctg gaacgc
//
LOCUS       DATASH010000819         6136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1523461,
            whole genome shotgun sequence.
ACCESSION   DATASH010000819 DATASH010000000
VERSION     DATASH010000819.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6136
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1523461"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="VHV99_21995"
     CDS             <1..632
                     /locus_tag="VHV99_21995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3638102.1"
                     /translation="HIVIGSSHAGRVEAAVTALGANAEGHALDLTDEHAIEAFFNMTG
                     SFDHLVFTAGDSLRLGEIASADLSLARRAFDIRYWGALAAVKHASPHIATDGSIVLTT
                     GIAALRPRSGWAFGASVCGAMEALTRALAVELAPLRVNAVSPGLIATNLWQNMSEADR
                     QAMYEQAGKHLPVGRVGEARDVAAAYLFLMESAFATGQTVVVDGGAVLV"
     gene            complement(764..5596)
                     /locus_tag="VHV99_22000"
     CDS             complement(764..5596)
                     /locus_tag="VHV99_22000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016354571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase subunit BcsC-related outer
                     membrane protein"
                     /protein_id="HEX3638103.1"
                     /translation="MLKRPLFPMKPHAGARAIALSLAAGCVANVPLTAFAQAASSAAN
                     AGAVTGGTAAGSGAAANSPLGVLIDQGRYWQSHRRGDLAEQAWQKVLRIDPKQPDALY
                     GMGIILADRKDGSGAQQYLQRLREAAPQYPNIDELGRRLGETSPRDQTVNDARRLAQS
                     GQSASAVQEYQRAINGKPATPELTLEYYQALAATPQGWDQARQGLTQLARENPNDPRY
                     ALALAQHLTYRDTTRRDGIARLAQLSTDSTVGEQAKKSWRQALLWLGARASDAPLYQA
                     YLQTSPDDPAVKARFDSMVQQDKQARDRAQENAAVDSRGRAVADGFAALDRGDIVTAR
                     ARFSAVLANSPNDADALGGMGIAALKQEKFDEARTYLERASRAGNPARWKDALTSATY
                     WSYTSAGIGARSNGEIAKAKSDFERAIALNPSDTTAQTMLGETLLNSGDPRGAEQAYR
                     MALRRQADNPDAIRGLVGALAAQGRGDEALSFANQLNAEQQAKAGGINKLRGEAQAAQ
                     ARAAEARGDLGTARSLFEDALLNNPDDVWMRLDLARIYVRQGAVGNARSMMDGLLAAH
                     PDMTDALYASALLSAETQDWATGLSQLERIPVNQRTTAMTVLQHRLWVHTQADLANRM
                     SRAGQTQQAEAILQAATPTAQGSPELIGVLASSYLQAGDSMRALSLVRGAMAAAPDDT
                     GLLLQYAGILTAVPVAAGQTNPQQEAELSSVMRRLAASQLTPQQRIDFNNLNIGIVVK
                     QADTVRQRGDLASAYDVIAPWLSAMPDNPDLQAALARLYTSAGDDRSALASYRAALAR
                     RPDDIDLQVAAIGAASGAKEFSFAEATANQALREAPEDPRVLAATGRMYHAEGKLTLA
                     SRYLQQALIAANTPVRQTARTAGQGTQPNVPRGWESAMQRIGSMPLPGTNPFEGKTAV
                     DTSAGAPSGAPTGPLSGLFTRSTAPMPGLPAGSSTYSQPSSPTQNVPNYLPPPQPAPF
                     STPYVAPYSTPGNVARPVATGGGYGPDTSGSSQSGGPAAPPLQPYPGQAPAQDSGQYQ
                     GQYQPQGQAYQQPQQPQYQQPYPAQNQQQYQQPYQPQAQQPQQPYPAQYQQTQQQVDP
                     AVSSPWPMSPAAREAQANAQQPQPHTYSSAPAAKSKHTSTRQQATSRNSRPADETDYR
                     QQPYPQQQQQYAQQGYGYPPPQPQQQQPYLNQGYGQPYIPQPPAGYGYAPAQPAQAYS
                     GMAGNNPTTIANAQTLGVADELAQINRARSSTVSGGVIFRSRTGEDGLSNLTDIEAPV
                     QGRIAAGNGHIVVTATPVTLDAGDAQSTADTLKRFGSGQAALSSPLVNNYGNQTATGV
                     GLSVGYETNSVKLDVGTTPLGFREENVVGGAQYNGSITDKVSYSIAVARRAVTDSLLS
                     YAGTRDQGQGLQWGGVTSNGGRMDLGWDDGTSGAYVNWAFQYYTGTNVASNTAEKGGG
                     GFYTRLFQDGNTTLTAGVNTTLMHYDKNLSYFTYGQGGYFSPQQYVILNLPVEYMGRN
                     GLFTYDLKGSIGVQHYRQDASNYFPITGNIQPGSSGAAGGVYSSSSKTGVSYSFSATG
                     EYQLAPQLTVGATASLGNAYQYREYLAAVYVRYSFTKQGGAVSFPPTPLTSPYLSLTN
                     "
     gene            complement(5590..>6136)
                     /locus_tag="VHV99_22005"
     CDS             complement(5590..>6136)
                     /locus_tag="VHV99_22005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013341569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HEX3638104.1"
                     /translation="QLAENSYKLVTTTAPHGFSPDWAAYKSGQFVVDPQTADTGSYDA
                     IRVYLWAGLASSADPLAKPWLAALGGMRRAVAQNGYPPERVNVATGAASGEGPLSYWA
                     ALAPYFKALGDDRGLGLARAHLAVLDAPVATSSGAAPANPDREPVYYDRVLGLFGAGF
                     IEGRYRFDEAGRLVPSWRSAC"
BASE COUNT         1017 a         1894 c         2016 g         1209 t
ORIGIN      
        1 cacacattgt gattggatcg agccatgcag gtcgtgtcga ggctgctgtc acggcccttg
       61 gcgcgaatgc cgaaggccac gcgctcgatc tcaccgatga acacgctatc gaagcgtttt
      121 tcaacatgac cggcagcttc gatcatctgg tgttcaccgc gggcgattcg ctgcggcttg
      181 gcgagatcgc ttcggcggat ttatcgctgg cgagacgcgc cttcgatatc cgctactggg
      241 gcgcgctcgc cgcggtcaag cacgcttcgc cgcatattgc gacggacggc tcgatcgtgc
      301 tgacaacggg catcgcggcg ttgcgtccgc gtagcggctg ggcattcggc gcgagtgtct
      361 gcggcgcaat ggaagcgctc acgcgggcgc ttgcagtcga actggcgccg ttgcgcgtga
      421 acgccgtgtc acccggactg atcgcgacga acctgtggca gaacatgtcg gaagcggacc
      481 ggcaagcgat gtacgaacaa gccggcaagc atttgcccgt cggacgagtg ggcgaggcgc
      541 gcgatgtcgc ggccgcgtac ttgtttctta tggagagcgc gttcgcaacg ggacagacgg
      601 ttgtggtgga tggaggggcg gtgttggttt aggggaggaa ggcgtgggct taagcgctgc
      661 agatacttgt attcaatcaa ctactcgcta gttcgcggca acaaaaaagt ggtttcggcg
      721 aactcgccca aaccactttt tttacgcctg attgatcacc ttatcaattc gttagcgaca
      781 gatagggcga ggtcagcggc gtcggtggga acgacactgc gccaccttgt ttcgtaaagc
      841 tgtatcgcac gtacacggcg gccaggtatt cgcgatactg atacgcattg ccaagagacg
      901 ctgtcgcgcc gaccgtaagt tgcggtgcaa gttgatattc gcccgtcgcg ctgaacgaat
      961 aagagacgcc tgttttgctg ctgctggaat aaacgcctcc ggcggcccct gaacttcctg
     1021 gctggatgtt gccggtgatt gggaaataat ttgacgcatc ctgccgatag tgctgcacac
     1081 cgatcgaacc cttgaggtcg taggtgaaca ggccgttacg gcccatgtac tcgaccggca
     1141 ggttcaggat cacgtattgc tgcgggctga agtagccgcc ctgcccatag gtgaagtacg
     1201 acaggttctt gtcgtagtgc atcagcgtgg tgttcacgcc cgccgtcagc gtggtgttcc
     1261 cgtcctggaa caggcgcgta tagaaaccgc cgccaccttt ttcggccgtg ttgctcgcga
     1321 cattggtccc ggtgtagtac tggaatgccc agttcacgta cgcgccgctc gttccgtcgt
     1381 cccaaccgag atccatgcgt ccgccgttcg acgtgacacc gccccattgc aagccttgac
     1441 cctgatcgcg cgtccctgca tacgagagca agctgtcggt gactgcccgg cgcgcaacgg
     1501 cgatcgagta cgaaaccttg tccgttatgc tgccgttgta ctgtgccccg ccgaccacgt
     1561 tttcttcgcg aaaaccaagc ggcgtagtgc cgacatcgag cttgacgctg ttcgtttcgt
     1621 atccgacgga caaacctacg ccggtcgccg tttggttgcc gtaattattg acaagaggcg
     1681 agctcaatgc ggcctgtccg gaaccaaagc gcttgagcgt gtcggctgtc gattgggcat
     1741 cgcccgcatc caacgtcacc ggagtcgcgg tcacgacgat atgcccgttc cccgccgcga
     1801 tgcgaccctg gaccggcgct tcgatatccg tcaggttcga cagcccgtcc tcgcctgtac
     1861 ggctgcggaa aatgacgccg cccgacaccg tgctagaccg ggcacgattg atctgcgcga
     1921 gttcatcggc gacgcccagc gtttgtgcgt ttgctatggt cgtcggattg ttacccgcca
     1981 taccggagta cgcctgtgcc ggttgtgcgg gagcgtagcc gtaaccagca ggcggttgcg
     2041 gaatgtaggg ctggccgtaa ccctggttca ggtacggctg ctgctgctgt ggctgcggcg
     2101 gcggataccc atagccttgc tgcgcatact gctgttgctg ctgcgggtaa ggctgctgcc
     2161 tgtagtccgt ttcatcggcc gggcgactgt tacgcgacgt ggcttgctgg cgcgttgacg
     2221 tatgtttcga tttcgccgcc ggcgcgctgg aatacgtgtg gggttgcggc tgctgcgcgt
     2281 tcgcttgcgc ttcacgcgca gcgggagaca tgggccaggg cgaactgacc gcggggtcga
     2341 cctgttgctg agtttgctga tactgcgccg ggtacggctg ctgcggctgc tgagcttgcg
     2401 gttgataagg ctgctgatac tgctgctgat tctgagccgg atacggctgt tgatattgcg
     2461 gctgctgcgg ctgctgataa gcctgcccct gcggctgata ttgcccctga tattgtcccg
     2521 aatcctgagc cggcgcttgc cccggatagg gctgcagcgg cggcgcagcc ggaccgccag
     2581 actggcttga acccgaagtg tcgggaccat atccgccgcc tgtcgctacc gggcgtgcaa
     2641 cattgcccgg cgtcgaatag ggagcaacat agggtgtcga aaaaggcgcg ggttgaggcg
     2701 ggggcaaata gttcggcacg ttctgcgtcg gtgaagaagg ctgggagtaa gtggacgatc
     2761 cagcgggcaa gccgggcatc ggcgcggtcg agcgcgtgaa cagaccgctc agcggcccgg
     2821 tcggtgcgcc cgatggtgcg cccgcagacg tatcgacggc ggtcttgcct tcaaaggggt
     2881 tggtgccggg cagcggcatc gacccgatgc gctgcatggc ggattcccag ccgcgcggca
     2941 cattgggttg cgtgccttgg ccagcagtac gcgccgtttg acgcacgggc gtatttgcag
     3001 cgatcaacgc ctgctgcaaa taccgtgatg ccagtgtcag tttcccttct gcgtgataca
     3061 tgcggccggt cgcggcgagc acgcgcgggt cctcgggagc ctcgcgcagc gcctgattcg
     3121 ctgttgcctc agcaaacgaa aattccttcg ccccggacgc cgcgccaatc gccgcaacct
     3181 gcaagtcgat gtcgtcagga cgtcgggcca gcgccgcgcg ataactcgcg agcgcgctgc
     3241 ggtcgtctcc tgcggaggtg tacaaacggg ccagcgcagc ttgcagatcg gggttatcgg
     3301 gcatggcgga aagccagggc gcgatcacat cgtaggcgct ggccaggtcc ccgcgctgac
     3361 gcacggtatc ggcctgtttc accacaatgc cgatgttcag gttgttgaag tcgatgcgct
     3421 gctgcggcgt gagctgcgaa gcagccagcc gtcgcatgac cgaactcagc tcggcttcct
     3481 gctggggatt cgtttgtccc gcggcaacgg gaacggcagt caggatacct gcatattgca
     3541 gcagcaggcc ggtatcgtcg ggagcagcgg ccattgcgcc gcgcaccagc gacaacgcgc
     3601 gcatgctgtc gcccgcctgc agatacgacg aagccagcac gccgatcagt tccgggctgc
     3661 cttgagcagt gggcgtagcg gcttgcagga tcgcctcggc ctgctgcgtc tgcccggcac
     3721 gcgacatccg gttcgcgagg tccgcttgcg tatgaaccca taagcgatgt tgcagcacgg
     3781 tcatggccgt cgtgcgctgg ttgaccggga tgcgttccaa ctgcgataaa cccgtcgccc
     3841 agtcctgcgt ttcggccgaa agcagcgcgc tcgcatagag cgcgtcggtc atgtccggat
     3901 gcgcggcgag caagccgtcc atcatgctgc gcgcattgcc gacagcaccc tggcgcacat
     3961 aaatgcgggc gaggtcgagg cgcatccaga cgtcgtcggg attgttcagc agcgcatctt
     4021 cgaagagact gcgcgcggtg cccaggtcac ctcgtgcttc cgcggcacgc gcctgcgctg
     4081 cctgcgcttc gccgcgcagc ttgttgatgc caccggcctt cgcctgctgt tccgcgttca
     4141 actggttcgc aaagctaagt gcttcgtcac cacggccttg cgcggccagt gcaccgacca
     4201 gtccgcggat cgcgtcaggg ttgtcggcct gacggcgcag cgccatccgg taggcttgtt
     4261 ccgcgccgcg agggtcgccg ctgttcagca acgtttcgcc gagcatggtc tgcgcggtgg
     4321 tgtccgacgg attcagcgcg atcgcgcgct cgaaatccga tttcgctttc gcgatctcgc
     4381 cattgctgcg cgcgccaatc cccgcgcttg tataactcca gtaagtcgcg ctggtgagcg
     4441 cgtctttcca gcgtgccgga ttgccggcac gcgaagcacg ttccaggtac gtgcgtgctt
     4501 cgtcgaattt ttcttgcttc aacgcagcga tgcccatgcc gccgagcgca tcggcatcgt
     4561 tcgggctatt cgccagcact gcggaaaaac gggcgcgcgc tgtgacgata tcgccacgat
     4621 ccaatgcggc gaaaccatcg gcgaccgcgc ggccgcggga atcgacagcg gcattctctt
     4681 gcgcacgatc gcgagcctgc ttgtcttgct ggaccatcga atcgaaacgc gctttgaccg
     4741 cgggatcgtc cggcgatgtc tgcaaataag cctgatagag cggggcgtcg gacgcgcgtg
     4801 cgccgagcca caggagagcc tggcgccagc tcttcttcgc ctgctcgccg acggtgctgt
     4861 cagtgctcaa ctgcgcaaga cgcgcaatgc cgtcgcggcg cgtggtgtcg cgataggtca
     4921 ggtgctgcgc gagcgccagc gcgtagcgcg ggtcgttggg gttttcgcgc gcgagctgcg
     4981 tgaggccctg acgcgcctga tcccagcctt gcggcgtggc ggcaagcgcc tggtaatact
     5041 cgagcgtgag ttcgggtgta gcgggcttgc cgttgatcgc gcgctgatat tcctgcactg
     5101 ccgatgcgct ctgaccactc tgcgcgagcc ggcgggcgtc gtttacggtc tggtcgcgcg
     5161 ggctggtttc gcccagccgc cggccgagtt catcgatatt cggatactgc ggcgcagctt
     5221 cccgaaggcg ctgcaagtat tgctgcgcgc cactgccgtc cttgcggtcc gccaggatga
     5281 tgcccatgcc atagagcgcg tcgggctgct tcgggtcgat gcgcagcact ttctgccacg
     5341 cttgttcggc cagatcgccc cgccgatgtg actgccagta gcggccctga tcgatcagca
     5401 cgccgagcgg cgaattggca gcagcaccac tgccggcagc ggtaccgccg gtaaccgcgc
     5461 ccgcattggc tgcgctgctg gcggcttgag cgaacgcggt cagcggcaca ttggcgacac
     5521 agcccgccgc gaggctcagc gcaatcgccc gcgcgccagc gtgtggtttc atggggaaca
     5581 gcggccgttt caacatgcac ttctccagct cggcacgagt cggccggctt cgtcgaaacg
     5641 gtatcggcct tctataaatc cggcgccgaa caagcccagc acccggtcgt aataaaccgg
     5701 ttcacggtca ggatttgcgg gcgcagcgcc cgaagaggtt gccacgggcg cgtcgagcac
     5761 cgcgagatgc gcgcgggcaa ggcccaggcc gcgatcgtcg ccgagcgcct tgaaatacgg
     5821 tgcgagcgcg gcccagtagc taagcgggcc ttcgccgctc gccgcccctg tcgccacatt
     5881 cacgcgttcc ggcggatagc cgttttgcgc gacggcgcga cgcatgccgc cgagcgccgc
     5941 gagccacggt ttggcaagcg gatcggcgct ggacgcaagc ccggcccaca gatacacacg
     6001 aatggcgtcg tagctaccgg tgtcggcagt ctgcggatcg acgacgaact gcccggactt
     6061 gtacgccgcc cagtcaggag aaaagccgtg cggcgcggtg gttgtgacga gcttgtagct
     6121 gttctcggcg agctgg
//
LOCUS       DATASH010000820         2174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3104499,
            whole genome shotgun sequence.
ACCESSION   DATASH010000820 DATASH010000000
VERSION     DATASH010000820.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3104499"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VHV99_22010"
     CDS             complement(<1..530)
                     /locus_tag="VHV99_22010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HEX3638105.1"
                     /translation="MNTFPDPRTRRFTLSAFATKWMGQSVEVGIVPVLVLLAIYLPFA
                     YFVIPESNALHLSLFGINLIGQIMCFALLALALDLVWGYAGVLSLGHGIFFGIGGYMM
                     AIYLLNGAYADTHVLPDFLQYMGASDFPAVWKMLSRLDVTIFATIVVTVLVAGAFGFV
                     TFRSRINGVYLSIITQA"
     gene            complement(527..2047)
                     /gene="urtB"
                     /locus_tag="VHV99_22015"
     CDS             complement(527..2047)
                     /gene="urtB"
                     /locus_tag="VHV99_22015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HEX3638106.1"
                     /translation="MLTSFCATDGDKAAALQQLVEAGVSDDTHERAWARQVVDAIRNG
                     ALVCGADGQATLQENPALDAATLQPRANAPADSPYVPSIVMMRRLAVASAEMDLFSPQ
                     QDTRATAARQVDKFFALLDPAVVARAVKAQHDPDIATILTLALAKRGLNSNVKADRLA
                     AIKAVAEVPSETARTALANFAAQDSVRSDPDVLAAVQGAIRHMDTWLSLGKGLSILFS
                     GLSYAGVLLLTALGLSIVFGLMGVINLAHGEFIMIGAYSTYLVERFMQAYLPGWFDAY
                     IFVAIPVAFVICAACGMLLEFFVIRFLYRRPLETLLATWAVSIALIKLIQVLFGSQNV
                     EFITPGFLNGGMQVAPGFFVTYNRVFAIVLAVLIFVATFAVVRFTKLGLLMRATTQLR
                     DMARCLGVPAERVDRIAFGLGAGLAGLAGVVLTQIASVNPSMGTGFVVDSFMVVVLGG
                     AGSLAGTVVSSLALGEINQFIEPFYGAVAAKVAVLLLIVLIIQKRPQGLFALKTRV"
BASE COUNT          497 a          696 c          647 g          334 t
ORIGIN      
        1 gcctgagtga tgatcgacag atacacgcca ttgatgcgcg agcggaacgt gacgaagccg
       61 aacgcgcccg cgaccagcac ggtgacgacg atcgtggcga agatcgtcac atccagccgc
      121 gacagcattt tccagaccgc gggaaagtcg ctcgcaccca tgtattgcag gaagtccggc
      181 aatacgtgcg tgtcggcgta ggcgccgttc agcagataga tggccatcat atagccgcct
      241 atgccgaaga agatgccgtg cccgaggctc aggacgcccg cataacccca taccagatcg
      301 agtgccagcg cgagcagcgc gaagcacatg atctgtccga tcaggttgat gccaaacagc
      361 gacagatgca gcgcattcga ctccggaatc acaaaatagg caaagggtag atagatcgcc
      421 agcaggacca gcaccgggac gatgccgacc tccacggact ggcccatcca tttcgttgcg
      481 aacgccgaca aggtgaatct cctggtacgc ggatcgggaa acgtattcat acgcgggtct
      541 tcaatgcaaa gagtccttgc gggcgcttct ggataatcag cacgatgagc agaagcacgg
      601 ccaccttggc ggcaaccgct ccgtagaacg gttcgataaa ctggttgatt tcgccgagcg
      661 cgagcgacga cacgactgtc cccgcgagac tgcctgcccc gccgagcacc accaccatga
      721 acgagtcgac gacgaagccc gtgcccatcg acggattgac gctggcaatc tgcgtcaaca
      781 ccacgccggc cagtcccgcg aggcctgccc ccagtccgaa cgcgatacga tcgactcgct
      841 cggcgggtac gccgaggcag cgcgccatat cgcgcaactg cgtggtggcg cgcatcagca
      901 aaccaagctt cgtgaagcga accacggcga aggtcgcgac gaagatcaac acggcgagca
      961 cgatggcaaa cacgcggttg taggtcacga aaaagccggg tgcaacctgc atgccgccgt
     1021 tgagaaagcc gggtgtgatg aactcgacgt tctgcgagcc gaacagcacc tgaatcagct
     1081 tgatcaacgc gatgctgacc gcccaggtag cgagcagcgt ctcgagcggc cggcgataca
     1141 gaaaccggat gacgaagaat tcgagcaaca tgccgcacgc tgcgcagatc acgaacgcca
     1201 cgggaatcgc aacgaaaatg taggcgtcga accagcccgg cagatacgcc tgcatgaaac
     1261 gttcgacgag atacgtggag tacgcgccga tcatgatgaa ttcgccatgc gcgagattga
     1321 tcacgcccat caggccgaac acgatcgaca ggccgagcgc cgtcagcaac aacaccccgg
     1381 cgtagctcaa gccgctaaac agaatcgaca agcccttgcc gagcgacagc cacgtgtcca
     1441 tgtggcgaat cgcaccctgc actgcagcca gcacgtccgg atccgagcgc acggaatcct
     1501 gcgccgcaaa gttcgctagc gccgtgcgcg cggtttcgct cggcacctcg gccacagcct
     1561 tgatcgccgc cagacgatcg gccttcacat tgctgttcag cccgcgttta gccagagcca
     1621 gcgtgagaat ggtcgcgata tccgggtcgt gctgcgcctt gaccgcacgg gcgacgacag
     1681 cgggatcgag caatgcaaag aacttgtcga cttgccgcgc agccgtcgcg cgggtgtcct
     1741 gctgtggcga gaagaggtcc atttccgccg acgccaccgc gagcctgcgc atcatcacga
     1801 tgctcggcac atagggcgaa tccgccggcg cgttggcgcg cggctgaagc gtcgccgcgt
     1861 cgagcgcagg gttttcctgc agcgtcgcct gaccgtctgc gccgcacacc agcgcgccgt
     1921 tacgaatcgc atcgacgacc tgacgcgccc acgcacgctc atgcgtatca tccgacacgc
     1981 cggcttcgac gagttgctgt agtgcagcgg ccttatcgcc gtccgtcgcg caaaacgacg
     2041 tgagcatcgc agcgcggccc ggcgcggcgc tcgcgctagt ggcccagaca agcgccaaac
     2101 aagcgatcgc gaaaaagatc tgcctgaaat tgatcatggg tgtttccatt cgatcctgct
     2161 acggggatcg tcaa
//
LOCUS       DATASH010000821         1923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5480588,
            whole genome shotgun sequence.
ACCESSION   DATASH010000821 DATASH010000000
VERSION     DATASH010000821.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5480588"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(281..371)
                     /locus_tag="VHV99_22020"
                     /pseudo
     CDS             complement(281..371)
                     /locus_tag="VHV99_22020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012492354.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            394..741
                     /locus_tag="VHV99_22025"
     CDS             394..741
                     /locus_tag="VHV99_22025"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX3638107.1"
                     /translation="MERFALLGDPQIGKAFADMVARLAAPHTVASLAECAYLSRSAFM
                     PRFSDALGQSPMQVLRDIRMRQAADQLRTGDYTLDQVARNAGYSSRGTFVRAFREVYG
                     TGPTEYRAESASA"
     gene            836..1309
                     /locus_tag="VHV99_22030"
                     /pseudo
     CDS             836..1309
                     /locus_tag="VHV99_22030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492679.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
     gene            1592..1870
                     /locus_tag="VHV99_22035"
     CDS             1592..1870
                     /locus_tag="VHV99_22035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638108.1"
                     /translation="MSRIDIPTPDQIPEGSKQILDTMTRQLHLTPNLFKIMALSPNAL
                     NGWAGLQGALAKTLDAKTLDLHSARRGHNLARISSLVRRAKGVFRDTE"
BASE COUNT          397 a          591 c          571 g          364 t
ORIGIN      
        1 ctgttcacga gcacgaccac gaatgccgcg cgcttcgttt cgctcgcacg tcgaaggagc
       61 gtgtgacaga tagtagtcag ggggggcgag agccagtagc atcgcgaatg cgtacaccat
      121 gttgtccgaa cggcaacaat ctacgacgga catgagaggc aatgcgagtg accgtctttt
      181 tgatcaacgt ttccttagga acgtcttcat ccatggggcg tcgatcccat taacgcctct
      241 atccggggcc gacagcgcgg attcatattt agggcccgtt tcataaaggc tgctcggtgc
      301 gccggagggt attccagcag atcaggcagc aaccgacttt gaggaaggcg gtgaatgtca
      361 acacggcgct cactcaggtc caacgcgctg tggatggaac gctttgccct gctcggtgat
      421 ccgcagatcg gcaaggcctt cgccgacatg gtcgcgcggc tggccgctcc gcacacggtg
      481 gctagcctgg ccgagtgcgc gtacctgagc cggtcggcct tcatgccccg cttctcggac
      541 gctctcggcc agtcgccgat gcaagtgctg cgcgacatcc gcatgcggca ggccgcggac
      601 cagttgcgca cgggcgacta cacgctcgac caggtcgctc gcaacgcggg ctacagtagc
      661 cgcggtacct ttgttcgtgc ttttcgcgag gtctatggca cgggcccgac cgaatatcgc
      721 gcggagtcgg caagcgcttg aaacggcgga cgatccgcac tgtctaccgg accctttgca
      781 aagcgttcgc ggctagactt cctataccga agcggaacct gaaaggagtt gcgaaatgtc
      841 tttcgaattc aaggttatgg aatcgcccgt aggcaagctc aaactcgttg cccgggacaa
      901 taagctcgtc gccattctct gggaaaacga tcgccccaat cgcgtgaagc tgggcgatat
      961 gcgcgaagtg ggtgacaacc cggtgctgct cgaaaccgcg cgtcaactcg gcgaatattt
     1021 cgccggcaag cggacgcgtt ttgatctgga catcgagttc gtcggcactg agttccagaa
     1081 gaaggtctgg gcagcgttgc tcacgatccc gttcggcgaa acgcgcagct atagcgacat
     1141 tgccaatcag atcggcaacg gccgcgcggt ccgggcggtg ggcgcggcaa acggccggaa
     1201 cccgctctcg atcatcgctc catgccatcg cgtgatcggt gcatcgggcg cgctgacggg
     1261 cttcgccggc ggactcgaaa ccaaggcgtt attgctcacg acggaagcca acgcgaacga
     1321 tcgccacgcg gcatgaacgc cgtatagaaa cggcaccatt cgcggcctgt ggcaggccgc
     1381 catcggcgcc ttgtattgac actccggtgt cgatgcaagg cgccgatttt ctttggtggt
     1441 cgccaccctg aagcgatgcg cacaaccgga cttttggccg cgtttggcgg accctctgca
     1501 aagctcgcgc acctggactt ctcatcaatg caggaacgca aacgcttcaa gcagatgccc
     1561 ttcaaaccga ttcaactctc aggagagttc catgtctcgc atcgacattc ccacgccgga
     1621 ccagattccc gaaggctcga agcagatcct cgataccatg acccggcaac tgcacctcac
     1681 gcccaacctg ttcaagatca tggccctcag tccgaacgcg ctgaacggct gggccggact
     1741 tcaaggtgct ctcgccaaga cgctcgatgc caagacgctc gacttacatt ccgcacgtcg
     1801 aggacacaat ttggcgcgaa tttcgtcgct agtccggcgg gcgaaaggcg tcttccggga
     1861 caccgagtag cctgagcagc tgacgctgca attccgtgag gggaacgtcg aagacctgca
     1921 cag
//
LOCUS       DATASH010000822         2817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3699502,
            whole genome shotgun sequence.
ACCESSION   DATASH010000822 DATASH010000000
VERSION     DATASH010000822.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3699502"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VHV99_22040"
     CDS             complement(<1..530)
                     /locus_tag="VHV99_22040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HEX3638109.1"
                     /translation="MSAIESVLQERRVFPPSAEAVGGATISGMDAYRALAAEAERDYE
                     GFWGRLARETLSWETPFTKVLDESKAPFYTWFEDGQINASYNSIDRHVEAGNGERVAI
                     IFEADDGTVTNVTYNDLLQRVSRFANALKKRDVKKGDRVVIYMPMSIEGIVAMQACAR
                     IGATHSVVFGGFSSKSL"
     gene            961..>2817
                     /locus_tag="VHV99_22045"
     CDS             961..>2817
                     /locus_tag="VHV99_22045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638110.1"
                     /translation="MTTSTGSATSAALTEQRLDAASDTVLPPDTPQSARRPPPIRDTS
                     SDSTDTGKASLRDRHGTSGDERVPPGSPQRSQGDGGVTADRNQRTPDSATTRTTPSSV
                     NLASAVSLARPVTVEPPSGRAAFSAPTPSTPSIPSTPSARPVLIGATQTELKQLLAAY
                     DTKTLPNMTHLSTSNDADEQNQAKLATDLNMSLKGYPPEVQQFEQTINDMQAQISTLP
                     QAEHQAYAGALATLDVAFRDSKDADGRRRADEQLSKLSDALIDRATIAENDPVERALG
                     VFNRPVGAGYLTDLGDRQQLSALGRLRAGFVTAATPAVRERYFALAAELKHNLQHKVG
                     TAIDQHTTQESSKWAAANGEVNRIIHEADMLTNDPGKRYELIGRQLYSTNPGSGRDEL
                     ADRRLLAFTQRMRDDPSLHGKLVNWSVEAGRKLNAAGVDGPKSYLDILNNLPPAGPDY
                     VRDLADQYNAVLHDTSYKDYSITPRARGEKLAEQVFEGATRFLLGLTPFAPVTAALDP
                     HSSLSPDTRLGIDLAAGLLGLFAGGGEAAFGERLAAKEAGAVFKAASGEHLPERPITT
                     GDKGAPLPAAPAIQSGQAGVQSARPAAQAAEQGLSVDAAVVEASQRISGTKAS"
BASE COUNT          565 a          951 c          843 g          458 t
ORIGIN      
        1 agcgacttcg acgagaagcc gccgaatacc accgaatgcg tggcgccgat gcgcgcgcac
       61 gcctgcatcg cgacgatgcc ttcgatcgac atcggcatat agatgacgac gcgatcgccc
      121 ttcttcacgt cgcgcttttt caacgcgttc gcaaagcgcg acacgcgttg cagcagatcg
      181 ttgtaggtga cgttggtgac ggtgccgtcg tcggcttcga agatgatcgc gacgcgctcg
      241 ccgttaccgg cttcaacgtg acggtcgatg ctgttatacg acgcgttgat ctggccgtct
      301 tcgaaccacg tgtagaaagg cgccttcgat tcgtcgagca ccttggtgaa gggcgtctcc
      361 cagcttagcg tctcgcgagc gaggcgcccc cagaagcctt cgtaatcgcg ttccgcttcg
      421 gcggcaagcg cccggtacgc atccatgccg gagatcgtcg caccgcccac tgcttcagcg
      481 gagggtggga aaacacggcg ttcctgaaga acagattcaa tcgcagacat cgacaacccc
      541 ttggtgaaga tgaaacttaa aacctgtgcc gacgcgccgg tggcgagcct tcgtgccgcg
      601 cgcaaatgac gcggcacgtg tctcctgatc cacggacaac ggggcgagca tgcggcactg
      661 cgtcacctga gcaacccctt ggtcggattg cagtcaagat aactgcagca acttacccgt
      721 cacttacgcc gcctgccctg cgccctgcat cggctcgctt gcgcaggttt cccatgttaa
      781 ctgaagcgct cgggccctgt catgaacttc gccctcgcat ggcatgcaaa gcgctgtcat
      841 acaggaatga cacccagggt tggtcctggg gtggtctcct gagcatgcat cgcaatcgat
      901 gcccatccgc gagcccggat cgcgcaggct cttcaccttg catggaacag gagcattcat
      961 atgacgacgt ccactggcag tgccaccagc gccgctttga ccgagcaacg tctcgatgcg
     1021 gccagtgata ccgttctgcc gcccgacacg ccacagtctg cgcggcggcc gccgcctatc
     1081 cgcgacacct catccgattc gactgacacc ggcaaggcat ccctacgcga tcggcatggc
     1141 acaagcggcg acgaacgcgt gccgccggga tcgccgcaac gatctcaggg tgacggcggc
     1201 gtcactgcag accgaaacca gcgcactccc gacagcgcta ccacaagaac gacgccgtct
     1261 tcagtgaatc tcgcgtcggc ggtgtcattg gcgagacccg tcacagtaga gccgccctcc
     1321 ggacgggccg cattctccgc gcccactccc tccactcctt ccatcccttc cacaccctcc
     1381 gcgcggccgg tactgatcgg cgccactcaa accgagctca agcaactgct cgccgcatac
     1441 gacacaaaga cgctgcccaa catgacgcat ctgtcgacga gcaacgacgc cgacgagcag
     1501 aaccaggcga agctggcgac cgatctgaac atgagcctca aaggctaccc gcccgaggtt
     1561 cagcaattcg agcagacgat caacgacatg caggcccaga tcagcactct gcctcaggcc
     1621 gagcaccagg cctacgccgg cgcgctggca acgctcgacg tcgcctttcg cgacagcaag
     1681 gacgccgacg gccgtcgacg cgccgacgaa caattgtcga agttgagcga cgcgctgatc
     1741 gaccgcgcca cgatcgcgga gaacgatccg gtcgaacgcg cattgggtgt cttcaaccgt
     1801 ccggtcgggg caggttatct gaccgatctg ggcgaccggc agcagctcag cgcattgggg
     1861 cggctacgcg cggggtttgt caccgccgcg acgccagctg tccgtgagcg ttatttcgcg
     1921 ctggcggccg aactgaagca caacctgcag cacaaggtcg gcacagcgat cgatcaacac
     1981 acgacccagg aaagcagcaa atgggcagcg gcgaatgggg aagtcaaccg gatcattcac
     2041 gaagccgaca tgctcaccaa tgatccgggc aagcgctacg aactgatcgg ccgtcagttg
     2101 tacagcacca acccgggctc tggccgcgac gagctggcag accgtcggct gctggcattc
     2161 acgcaacgga tgcgggacga cccctcgctg cacggcaagc tcgtcaactg gtcagtcgag
     2221 gcgggacgaa aactcaacgc cgctggcgtc gatggaccca aaagctatct ggacattctg
     2281 aataacctgc cgcccgccgg gccagactat gtccgcgatc tggccgacca gtacaacgcc
     2341 gtgctgcacg atacgtcgta caaggactat tcgatcacgc cacgcgcccg tggagaaaag
     2401 ctggccgagc aggttttcga aggcgccacg cgttttctgc tcggcctgac gccgttcgct
     2461 cctgtcacgg ccgcgctcga cccgcattcg tcgctgtcgc cagacacccg gctaggcatc
     2521 gacctcgcag ccggcctgct cgggctcttc gcgggcggag gcgaggccgc tttcggcgag
     2581 cggcttgccg cgaaggaggc cggagccgtc ttcaaggcgg cttccgggga acatctcccc
     2641 gagcgcccga tcacgacggg cgacaaaggg gctccgctcc cggccgcgcc agccatccag
     2701 tccggccagg caggcgtgca aagcgcgcgg ccggcggccc aagccgctga gcaaggtctg
     2761 agtgtggacg cggcagttgt ggaggcgtcg cagcgcatca gcggtacgaa ggcgagt
//
LOCUS       DATASH010000823         3256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2117892,
            whole genome shotgun sequence.
ACCESSION   DATASH010000823 DATASH010000000
VERSION     DATASH010000823.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2117892"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..1148)
                     /locus_tag="VHV99_22050"
     CDS             complement(66..1148)
                     /locus_tag="VHV99_22050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX3638111.1"
                     /translation="MISRPPVAPPESPAPPDRLKKQKTALLVLYIALVLLALWVVRDF
                     IAVAAWAGVIAIALWPLLRKLEANRWFAGRTTLVAAVLTLAIALLVVLPIGIGITEAL
                     REAHDVNEWFKTAQENGIPMPDIIERLPFGVQQISSWWQANLAPPLRDSAAMKGLHST
                     TVMTLGRHFGARAARAVMVFGFMLITLFVIFQAGPRLSGSLMRGVRRCFGDDGAHLLG
                     RMAAGVRGTVSGLVVVGLGEGVLMGVAYFVTGLPHVALLAFITAIAAMLPFCAPITFG
                     LAALWLFSQGAVAAAIGLAVFGSVVVFVAEHFVRPVLIGNSTRLPFLLVLFGILGGAE
                     TFGLLGLFIGPALMTVLMVLWTDLVQ"
     gene            1494..1778
                     /locus_tag="VHV99_22055"
     CDS             1494..1778
                     /locus_tag="VHV99_22055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3331 domain-containing protein"
                     /protein_id="HEX3638112.1"
                     /translation="MTLESIPLDGTNGVRIEILERSDTTLVIRWVEPGRCHYGEQRWR
                     RRSAHTSGTCAVTRRKIRRGDAVFKPAERPAPSNASAMICAEVLCELAEA"
     gene            2215..>3256
                     /locus_tag="VHV99_22060"
     CDS             2215..>3256
                     /locus_tag="VHV99_22060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HEX3638113.1"
                     /translation="MAEDTHETRAAAVPSVQLVAPQQFSLDVLLEKYAKGDEQTADDV
                     FKRVARGVAQAESEALREAVEARFVDNLRHGALGAGRIMSAAGTGIAATLINCFVQPV
                     GDAIQGVDEQGLPGIYVALLQAAETMRRGGGVGYNFSAIRPKGARVHTTSSSASGPCS
                     YMDVFDASCRTVESAGSRRGAQMAVLDCNHPDLIEFIEAKHSKGRWNNFNVSVGVTDD
                     FMRAVEDDQPWQLVHRADPSPALRAAGDVRQREDGLWVYSERPAREIWDRIMRSTYDV
                     AEPGIVFISRMNEDNNLRAVETIRATNPCGEQPLPAYGCCNLGPLNLTRFVLDPFAQM
                     KGRKPSFDWDGLA"
BASE COUNT          678 a         1099 c          934 g          545 t
ORIGIN      
        1 gatctattca cgtgcggctc gaatcgccag atcatgcgcg attcggacca ccaactaacg
       61 tgcgactatt gcacgagatc ggtccacagc accatcagca cggtcatgag cgcggggccg
      121 atgaaaagtc ccagcaaacc gaacgtctcc gccccgccga gaatgccgaa aagcaccagc
      181 aggaaaggta gacgtgtcga gttaccgatc aacaccggcc gcacgaaatg ttcagcgacg
      241 aacaccacga ctgagccgaa cacggccagc ccgatggccg cggcaaccgc accttgcgag
      301 aacagccata aggccgcaag gccaaacgtg atcggcgcgc agaacggcag catcgcggcg
      361 atcgccgtga tgaacgcgag cagcgcgaca tgcggcaagc cggtcacgaa ataggcgacg
      421 cccatcaaca cgccctcgcc aaggccgacc accaccagcc ccgacaccgt gccgcgcact
      481 cccgcggcca tgcgcccgag caggtgcgcg ccgtcgtcgc cgaagcagcg ccgcacgcct
      541 ctcatcaacg agccggacaa acgcgggccg gcctgaaaga tcacgaacag cgtgatcagc
      601 atgaagccga acaccatcac agcgcgcgcc gcgcgcgcgc cgaagtggcg gccgagcgtc
      661 atcacggtcg tgctgtgcaa ccccttcatt gccgccgaat cgcgcagcgg cggggccaga
      721 ttggcctgcc accagctcga aatctgttgc acgccaaacg gcaaacgctc gatgatgtcc
      781 ggcatcggaa tgccgttttc ctgcgcggtc ttgaaccact cgttcacgtc atgcgcttcg
      841 cgcagcgcct cggtaatgcc gataccgatc ggcagcacaa ccagcaatgc gatcgcaagt
      901 gtcagtaccg cggcaaccag cgtcgtgcgg cccgcgaacc agcggttagc ctccaacttt
      961 ctcaaaagcg gccacagcgc aattgcaatg acgcctgccc aggccgccac cgcgatgaaa
     1021 tcgcgcacga cccaaagcgc cagcaacacg agtgcgatgt acagcacgag caatgccgtt
     1081 ttctgttttt tcaggcgatc aggcggtgcc ggcgattcgg gtggcgctac cggggggcgt
     1141 gaaatcatgt gggctcttag ggtcgtgtgg atatgcgcgc gtgccggccg gaaaaatgca
     1201 aacccgctac acttgcatcg cacggtcgct catcaataac cacatcttaa aggaaggacc
     1261 ggcgcccttt gctggcggca ttctcgtcgc accgagcaca acactgcgtt gcaaaaatcc
     1321 accctatcgt aaattcacct cgcatatggc cagcaaagcc ccgtcgttac gggtaagtgc
     1381 ggatgtcaag tttcattgtt tctatttaga gaacagtgtt tcaaccgcgg cacaatggct
     1441 atccggcgcg cgcgggacta cattcggcac accacaatac ggagccgacg atgatgacct
     1501 tagagtccat cccccttgac ggtaccaatg gcgtacgcat cgaaattctc gagcgctccg
     1561 acacgacgct ggtgatccgc tgggtcgaac cggggcgttg tcactatggc gaacaacgtt
     1621 ggcggcggcg ctcggcgcat acctcgggca catgtgctgt cacgcggcgc aagattcgcc
     1681 gcggcgatgc ggtgttcaaa ccggcagaac ggccagcgcc ttccaacgcg tccgcgatga
     1741 tttgcgctga agttttatgc gagcttgctg aagcgtaagt tttttgtatg cgcggcactt
     1801 cgctgccgtg cgcttgcggc gccagccgcc gttggcctcc cacgtgagtt gtttgcgcga
     1861 gcttgccgcc accccctttt ttcggcgatg tctctcacac gctcggcagc gctcgcagct
     1921 tgagcacctt gccactcacc gcctcagcgc ctcaccacct caccacctca ccacctcacc
     1981 gcctcaccac ctcaccacct tataaaggca gcgcgaaagt ccactgcaac tggcggacgg
     2041 tcgatccgcg gcccagtccc caaacgcccg attgcggctc ttcgccacgt gcgctatggt
     2101 ggtccacgat gcgccaggtc accatgcctg ccagatgcgc accgttaaat gaatgccctg
     2161 catcccattc gaacgcgccg cagcccgtct cgcggcaaac gagaggcgac acccatggca
     2221 gaagacactc acgaaacccg cgccgcagcg gttccctccg tccagttagt tgcaccgcaa
     2281 caattctcgc tggacgtcct gctcgagaaa tacgcgaaag gcgacgagca aaccgccgac
     2341 gacgtgttca aacgcgtcgc ccgtggcgtc gcccaggcgg aatctgaagc attgcgcgag
     2401 gcggtggaag cgcgctttgt cgacaatctg cggcacggcg cactgggcgc cgggcgcatc
     2461 atgagcgcgg caggcacggg catcgccgcc acgctcatca actgcttcgt gcaaccggtc
     2521 ggcgacgcga ttcaaggtgt cgacgaacag ggccttcccg gcatctatgt ggcgttgctg
     2581 caggccgccg aaacaatgcg ccgcggtggt ggcgtcggtt acaacttctc cgcgatccga
     2641 cccaagggtg cgcgcgttca taccaccagc tcatcggcgt ccggaccgtg cagttatatg
     2701 gacgtcttcg acgcctcatg ccggaccgtc gaaagtgccg gctcgcggcg cggcgcgcaa
     2761 atggccgtgc tcgactgcaa tcaccccgat ctgatcgagt ttatcgaggc caaacattcg
     2821 aaaggccgct ggaacaactt caatgtgtcg gtgggcgtga ccgacgactt catgcgcgcc
     2881 gtcgaagacg atcagccatg gcaactcgtg catcgcgccg acccgtcgcc ggcgttgcgt
     2941 gcggccggcg acgtccggca acgcgaggac ggcctgtggg tttacagcga acggccggcc
     3001 cgcgagatat gggatcgcat catgcgctcc acctacgacg tcgccgagcc cggcatcgtg
     3061 ttcatctcgc gcatgaacga ggataacaat ctgcgcgccg tcgaaaccat ccgcgccacc
     3121 aacccgtgcg gtgaacaacc gctgccggcc tacggctgct gcaacctcgg accgttgaac
     3181 ctcacgcgtt tcgtgctcga tccgttcgcg caaatgaaag gccgcaagcc ttcgttcgac
     3241 tgggacggtc tcgcca
//
LOCUS       DATASH010000824         8718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2118023,
            whole genome shotgun sequence.
ACCESSION   DATASH010000824 DATASH010000000
VERSION     DATASH010000824.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 8718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8718
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2118023"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2112
                     /locus_tag="VHV99_22065"
     CDS             <1..2112
                     /locus_tag="VHV99_22065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HEX3638114.1"
                     /translation="SHVTCPRCNGTGHIRDTESSALQVLRIIQEEAMKENTAAIHCQV
                     PVEVTAFLLNEKRAEINKIESRFKVNVVLIPNKHLDTPHYKLERLRHDDARLDEPRAS
                     WKMAEEAARELESETGYSKRAEEVKPKQEAAVKGITPEKPAPSAPVRPVATPAPVAVT
                     PAGGGFIGWLKNLFGMQPAAPAPAAPATTEKQTRPQRGERTERGERTGERGGDRNRNR
                     RGGVGSRDAAGRAEGTTGGRQAQAQQPSQRRDEREPREGREAREGREPREARETREPR
                     GNREPREVREGREPREAREPREGREGRENRDRDNRGNDRAAETVDGAPRAERGERQER
                     GERRERGERAERGERRKQQPEAADAQAQNDALTAEELAQNQAALGENGAPVDQEAVAR
                     EGEERRRRRRGRRGGRREREEDVNGNLAADVAEAEGDNANVTDEAPLRTAHQPVEHKA
                     EANEVKPVEVVVAAVATETAVVSELHAAVETPALAVEKAARTETVVPVAEAPVTPVAV
                     EQAAQAPLAQVAAAPVEAAQAAPAVSETVSLVEPVAHVEAPVEARVETPVEARVETPA
                     VAPVVEAQAAPAPVETAAPAAVVEAPAAQPAPQAEIVEPQPVVAAVAPAVEPAAIEPA
                     PVAVAPQTPRNGGVAEEALKPVLEQAGLVWVNTDADKLRAAQEAAAQAAKPVRVGRER
                     KALPPVETAPMQMVETGKHPQ"
     gene            2373..3482
                     /gene="moaA"
                     /locus_tag="VHV99_22070"
     CDS             2373..3482
                     /gene="moaA"
                     /locus_tag="VHV99_22070"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012213801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEX3638115.1"
                     /translation="MSRRIIPLADLSAVPVISGPVQAPMGALHDTLARPLRDLRISVT
                     DRCNFRCVYCMPRAVFDKHYEFLPHGALLSFEEIERLARLFVAHGVEKIRLTGGEPLL
                     RKNLEHLIERLAQLTTPEGRPLDLTLTTNGSLLERKARSLKDAGLTRVTVSLDALDDT
                     LFRRMNDADFAVADVLDGIAAAHAVGLAPVKVNMVVKRGTNDSQIVPMARHFKDSGAV
                     LRFIEYMDVGTSNGWNMTEVLPSAEVVTRIAEHFPLVPLEAHSAAETAQRWGYVDGSG
                     EVGVISSVTRAFCGACTRARLSTEGKLYLCLFASSGHDLRALLRSGESDADIATAVAE
                     IWQGRSDRYSQLRGSSAAAPREQDCRRVEMSYIGG"
     gene            3495..4109
                     /gene="mobA"
                     /locus_tag="VHV99_22075"
     CDS             3495..4109
                     /gene="mobA"
                     /locus_tag="VHV99_22075"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HEX3638116.1"
                     /translation="MNPTRDHITGLVLAGGRGMRMGGLDKGLQPLHGEPLAAHVLKRL
                     APQTGALLISANRHPDVYSTLGAPFGAKVLADTLPGFPGPLAGLLAGLRTAETAYVLS
                     APCDSPGLPAELADRLAQALHSNQADIATVTTTDAAGHVSLHPVFALLRTGLADDLAS
                     FLEAGERKVRAWYVRHRTVEVAFTDERAFYNINSLQELADLERC"
     gene            4185..5486
                     /gene="glp"
                     /locus_tag="VHV99_22080"
     CDS             4185..5486
                     /gene="glp"
                     /locus_tag="VHV99_22080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEX3638117.1"
                     /translation="MTTLNEFSRCVTQYDPHALPVPAAQAIVRQWATPVTAVERVPLR
                     DALNRVLAADIVSPIDVPSHDNSAMDGYAFNRAALATNESTIDLTIVGKALAGHPFAG
                     QVGTTQCVRVMTGACMPADCDSVVPQELVERSADSTSIRFAANAVRAGANRRLAGEDL
                     ARGHAALRAGRIMRASDLGLLASLGIGEVNVRRRLRVAFFSTGDELRSLGEPLDAGSV
                     YDSNRYTLFAMLRRLNMETLDLGVVRDEPAALEAALRSAAASADVVLTSGGVSVGEAD
                     FTRQLLQTFGDVAFWSLAMRPGRPLAFGRVWSGEHPGLGQPALFFGLPGNPVAVMVAF
                     YQIVREVLLLMSGATPQALPVLRAASRQAIRKRAGRTEFQRGVAERDAHGQWHVTPTG
                     SQSSGVLSSMSEANCFIVLGHDEGEIAEGEHVDIMLFDCLI"
     gene            5601..5876
                     /locus_tag="VHV99_22085"
     CDS             5601..5876
                     /locus_tag="VHV99_22085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638118.1"
                     /translation="MKKQISFIAPGQTAKALILVYLTFSVPIVLLGVLVAFIRYGSVE
                     LSTVFSALLLNAILGFVLLWIACHAYNWVASRFGGIEIQLTDAPEEA"
     gene            5876..6364
                     /locus_tag="VHV99_22090"
     CDS             5876..6364
                     /locus_tag="VHV99_22090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3638119.1"
                     /translation="MSATIRAATPADTGAIFALTYELAEFESLTHVFVATEDGLRDAL
                     FGARPSIEALVAEDEGRLVGYALFFHNYSSFVGKRGLYLEDVYVQPGQRGSGLGTAIL
                     TRLAALAVERQCARFEWTVLDWNKQAISFYEKMGATVMPDWRVVRLAGDALERLARVA
                     AS"
     gene            complement(6443..7891)
                     /gene="rmuC"
                     /locus_tag="VHV99_22095"
     CDS             complement(6443..7891)
                     /gene="rmuC"
                     /locus_tag="VHV99_22095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEX3638120.1"
                     /translation="MTMILVAAVAVLAVALVIALALLMRGHGRADESERFEILSERID
                     AAADAQSHAYERLERQLRNDITETARVSRTEQSSGFAHFQQTLASQFSSMTTVQGGKI
                     DGFAQQLDAVRHSLQQQAQQARDEQGRTLRQFGETLSLQLGQLTEANDRRFAEVRATI
                     EQRLKDIEANNSTRLEEMRRTVDEKLHATLEQRLGESFKLVSDRLEQVHRGLGEMQTL
                     AAGVGDLKKVLTNVKTRGTWGEVQLEALLEQLLTADQYAKNVATVPKSADRVEFAIKL
                     PGRAATGESATPVWLPIDAKFPREDYERLIEAQERADPVAVEDASRALEARIRAEART
                     IAEKYVAPPHTTDFALLFLPTEGLFAEVLRRPGLTDQLQRDYRVTIAGPTTLTALLNS
                     LQMGFRTLAIERRSSEVWQVLGAVKTEFGKFGDVLARTKAQLETVTRSIEAAETRTRV
                     MNRKLRDVEALPGEEASGLLGDALPGVDPDEQ"
     gene            complement(7888..8166)
                     /locus_tag="VHV99_22100"
                     /pseudo
     CDS             complement(7888..8166)
                     /locus_tag="VHV99_22100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433401.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            8140..8433
                     /locus_tag="VHV99_22105"
                     /pseudo
     CDS             8140..8433
                     /locus_tag="VHV99_22105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433401.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            8430..>8718
                     /locus_tag="VHV99_22110"
     CDS             8430..>8718
                     /locus_tag="VHV99_22110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEX3638121.1"
                     /translation="MIMILVAAVAVLAVALVIALALLMRGHSRAEDSEQFDLLNERMD
                     AAADTQAHAYERLERQLRNDITETARVSRTEQSGGFAHFQQTLAAQFSSMTT"
BASE COUNT         1552 a         2859 c         2817 g         1490 t
ORIGIN      
        1 agccacgtga cctgcccgcg ctgtaacggc acgggccaca tccgcgatac cgaatcgtcc
       61 gcgctgcaag tgctgcggat cattcaggaa gaagcgatga aggaaaacac cgcggcgatc
      121 cattgccagg tgccggtcga agtgaccgcc ttcctgttga acgaaaagcg cgctgaaatc
      181 aacaagatcg agtcgcgctt caaggtcaac gtcgtcctga tcccgaacaa gcacctcgac
      241 acgccgcatt acaagctcga gcgcctgcgt cacgacgatg cgcggctcga cgaaccgcgc
      301 gcctcgtgga agatggctga agaagcagcc cgcgaactgg aatcggaaac cggttacagc
      361 aagcgcgccg aggaagtgaa gccgaagcag gaagcggcgg tcaagggcat cacgcctgag
      421 aagccggccc cgagtgcgcc ggtccgtccg gtggcaacgc cggctccggt ggctgtcacc
      481 ccggctggcg gtggctttat cggctggttg aagaatctgt tcggtatgca gccggccgca
      541 ccggcgcctg ccgcaccggc tacgaccgaa aagcagacgc gtccgcaacg cggcgaacgc
      601 acggagcgcg gtgagcgcac cggcgaacgc ggcggcgatc gcaaccgcaa tcgccgtggc
      661 ggcgttggca gccgcgatgc agcaggccgt gccgaaggca cgaccggcgg ccgtcaggct
      721 caagctcagc agccgtcgca gcgtcgcgac gaacgcgaac cgcgcgaagg ccgtgaggca
      781 cgcgaaggtc gtgaaccgcg tgaagcacgt gaaacgcgcg agccgcgtgg caatcgtgag
      841 ccgcgtgagg tccgcgaagg ccgtgaaccc cgcgaggctc gtgagccgcg tgaaggtcgc
      901 gagggccgcg aaaaccgtga ccgcgacaat cgcggcaacg atcgtgcggc ggaaaccgtc
      961 gacggcgcac cgcgcgccga gcgtggcgag cgccaggaac gcggcgaacg tcgtgagcgt
     1021 ggcgaacgcg ctgaacgtgg cgaacgccgc aagcaacagc cggaagcggc ggatgcacag
     1081 gcgcaaaacg acgcactgac ggcggaagaa ctggcgcaaa accaggccgc cttgggcgaa
     1141 aacggtgcgc cggtcgacca ggaagcggtg gcgcgtgaag gtgaagagcg ccgtcgtcgc
     1201 cgtcgcggcc gccggggtgg tcgtcgtgag cgtgaagaag acgtgaacgg caatctggct
     1261 gcggacgtcg cggaagctga aggcgacaac gccaatgtga ccgacgaagc gccgctgcgt
     1321 accgcgcatc aaccggttga gcacaaagct gaagccaacg aagtcaaacc ggtggaagtg
     1381 gttgtcgctg ccgttgcgac ggaaaccgct gtcgtcagcg aactgcatgc tgcagtcgaa
     1441 acgccggctc tggccgtcga gaaggctgcc aggacggaaa cggttgtgcc ggtcgcagaa
     1501 gcgccggtga caccggttgc cgtcgagcaa gcggcgcaag cgccattggc gcaagttgct
     1561 gccgcgccgg tcgaagccgc ccaggctgca ccggccgtga gcgagacggt ttcgctcgtc
     1621 gaaccggttg ctcacgtcga agcgcccgtc gaagctcggg tggaaacgcc cgttgaagct
     1681 cgggtcgaaa cgccggcagt tgcgccggtc gtcgaagcgc aagccgcacc ggctccggta
     1741 gaaaccgcag ctcctgcggc tgtggtcgaa gcaccggcag cccagccggc gccgcaagcc
     1801 gaaatcgtcg aaccgcagcc ggtagttgca gcggtcgctc cggcagttga gccggcagca
     1861 atcgagccgg cacccgtcgc agttgctccg caaacaccgc gtaacggcgg tgtggcggag
     1921 gaagccctga agccggtgct ggaacaagcc ggtctcgtct gggtgaacac ggacgccgac
     1981 aagctgcggg ccgcgcagga agctgctgcg caagcggcca agccggttcg cgtgggtcgc
     2041 gagcgtaagg cgttgccgcc ggttgaaacc gcgccgatgc aaatggtgga aaccggtaag
     2101 cacccgcagt aagcgaaccg ccgccacgca aaaagcccgc cctcatcggc gggctttttg
     2161 tttttatgga cgccgcggtt tttatggacg ccgcgcggcc aacccttgtg gcgcgcctta
     2221 tgcggctccc cgtatacccc gacagcgtcc gcagtccggc agccgccgaa tgcatttccg
     2281 ctagaatgaa gatccaggtt ccaatcgcag cgccaagcag caaccaggca gcacctgagc
     2341 agccccaaac gaagcatcga gcgaagcaat tcatgtcccg acgtatcatc cctcttgccg
     2401 atctcagcgc ggtgccggtc atctccggcc cagtgcaggc gcctatgggc gccctgcacg
     2461 acacgctggc gcgcccgctg cgcgatctgc gcatatcggt gacggatcgc tgcaatttcc
     2521 gctgcgtcta ctgtatgccg cgcgcggtgt tcgacaagca ctacgagttc ttgccgcatg
     2581 gcgcgctgct cagcttcgaa gaaatcgaac gattggcgcg gctcttcgtc gcacatggcg
     2641 tggagaaaat ccgccttacg ggcggcgagc cgctgctgcg caaaaatctg gaacacctga
     2701 ttgaacggct cgcgcaactc accacgccgg aaggccgccc actcgatctg acgctgacca
     2761 ccaacggttc actgctggag cggaaggcgc gcagcctgaa agacgccggc ctgacccgcg
     2821 tgacggtgag tctcgacgcg ctcgacgaca ccctgtttcg gcgcatgaac gacgccgact
     2881 tcgcggtcgc cgacgtgctg gacggaatcg ccgcggccca cgcggtaggg ctcgcgccgg
     2941 tgaaggtcaa catggtcgtc aagcgcggca ccaacgacag ccagatcgtg ccgatggcgc
     3001 gtcacttcaa agactcgggt gcggtgttgc gtttcatcga gtacatggac gtgggcacgt
     3061 cgaacggctg gaacatgacc gaagtgctgc cttccgccga ggtcgtgacg cggatcgccg
     3121 aacatttccc gctcgtaccg ctcgaggcgc acagcgcagc cgaaaccgcc cagcgctggg
     3181 ggtacgtgga cggcagcggc gaagtcggcg tgatttcgag cgtgacacgc gcgttttgcg
     3241 gcgcctgtac gcgcgcgcgt ctgtcgaccg agggcaagtt atacctgtgc ctgttcgcgt
     3301 cgtccggtca cgatctgcgc gcgttgctgc gcagcggcga gagcgacgcc gatatcgcca
     3361 cggccgtcgc cgaaatctgg caaggccgca gcgaccgtta ttcgcaattg cgcggcagca
     3421 gcgccgccgc gccgcgcgag caggactgtc gccgcgtgga aatgtcgtat atcggcggct
     3481 aaacggccga atccatgaac ccgacacgcg accacatcac cggtctggtc ctcgcaggcg
     3541 gacgcggcat gcgcatgggc gggctcgaca aagggctgca accgctgcac ggcgagccgc
     3601 tcgcggccca tgtgctgaag cggctcgcac cgcaaaccgg cgccttgctg atcagcgcca
     3661 atcgtcaccc tgatgtctac agcacactcg gcgcgccgtt cggcgccaag gtgcttgccg
     3721 ataccctgcc cggcttcccc ggcccgctgg cgggcctgct ggccggactg cgcaccgccg
     3781 aaaccgcgta cgtgctgagc gcgccctgtg attcgccggg actgcccgcc gaacttgccg
     3841 accggctcgc gcaggcgctc cactcgaatc aggctgacat cgccaccgtc acgaccactg
     3901 acgccgcggg ccacgtctcg ctccatcccg tgttcgcgct gctgcgcacg ggtctcgcag
     3961 acgacctcgc aagctttctg gaagccggtg agcgcaaggt ccgcgcgtgg tacgtacgcc
     4021 acaggacggt cgaagtcgcc tttaccgacg agcgcgcgtt ttacaatatc aattcgttgc
     4081 aagaactcgc cgacctcgag cgttgctgag gccccggttc aaatcccgct ccggccgcac
     4141 ggcaacagcc gcttcccgcc tctggtcgcg acgtctaccc cttcatgacc acgcttaacg
     4201 aattttcccg ctgcgtcacg cagtacgatc ctcacgcgct acccgtcccg gccgctcagg
     4261 cgatcgtccg tcaatgggcg acgcccgtca cggcggtcga acgcgtgcct ttgcgcgacg
     4321 cgctcaatcg cgtgctcgcg gccgacatcg tctcgccgat cgacgttcct tcgcacgaca
     4381 actcggccat ggacggctac gcgttcaatc gcgcggcctt ggcgacaaac gagtcgacga
     4441 tcgatctgac gatcgtcggc aaggcgctgg ccggccatcc gttcgccggc caggtgggca
     4501 caacgcaatg cgtgcgcgtc atgacgggtg cctgcatgcc ggctgattgc gacagcgtcg
     4561 tgccgcagga actcgtcgag cgtagtgccg attccacctc gattcgcttt gcggccaatg
     4621 ccgtgcgggc cggcgccaac cggcgtctgg ccggtgaaga tctcgctcgc ggtcacgccg
     4681 cgctgcgcgc cggccgcatc atgcgcgcgt cggacctcgg tttgctggcg tcgctcggca
     4741 ttggcgaagt caacgtgcgg cggcgtttgc gcgtcgcatt tttctccacc ggcgacgaat
     4801 tgcgctcact cggtgagccg ctcgacgcgg gttcggtgta cgacagcaat cgctacacgc
     4861 tgtttgcgat gctccggcgc ctgaacatgg agacgctcga tctcggggtg gtgcgcgacg
     4921 aacccgccgc gctcgaagca gcgctgcgca gtgcggcagc cagcgccgac gtggtgttga
     4981 cctcgggcgg cgtttccgtt ggcgaagcgg atttcaccag gcaattgctg cagacgttcg
     5041 gcgacgtcgc cttctggagt ctcgccatgc gcccaggccg gccgctcgca tttggccgcg
     5101 tctggtccgg cgagcatccg ggcctcgggc agcctgcgct attttttggc ttgccgggca
     5161 atcccgtggc tgtgatggtg gcgttctatc agatcgtccg cgaagtgctg ctgttgatgt
     5221 ccggcgcgac gccgcaggca ttgccggtct tgcgcgccgc cagccgccag gcaatccgca
     5281 agcgcgccgg ccgcaccgag ttccagcgcg gcgtggccga gcgggatgcg catggtcaat
     5341 ggcatgtcac gccaactggc tcgcaaagct ccggcgtgct gagttcgatg agcgaggcca
     5401 attgcttcat cgtgctcgga cacgacgaag gcgagattgc cgagggtgag cacgtcgata
     5461 tcatgctgtt cgactgtctc atctgatgac gtactgagcg cttctgttgt ggcctcgttt
     5521 ctagccggat ttgcagccgg atttccagcc tcattttgca gctttaccga ttaccacttc
     5581 gacacacggg tttgccttac atgaaaaaac agatctcgtt cattgcaccg ggacagacag
     5641 ccaaagcact gatcctcgtg tacctgacgt tctcggtacc gatcgtgctg ctcggcgtgc
     5701 tggtggcgtt catccgctac ggctcggtcg aactgagcac ggtgttcagc gccctgctgc
     5761 tgaacgcgat tctcggcttc gtcctgctgt ggattgcgtg ccacgcgtac aactgggtgg
     5821 catcgcgttt cggcggcatc gaaatccagc tgaccgacgc gccggaagaa gcgtaatgtc
     5881 cgcaacgatc cgcgccgcga cgcccgcgga tacgggcgca atcttcgcgc tgacgtacga
     5941 actggccgaa ttcgaaagcc tcacgcacgt attcgtcgcg actgaagacg gtttgcgtga
     6001 cgcgctgttc ggcgcgcggc cctcgatcga agcattggtg gcggaagacg aaggacgcct
     6061 cgtcggctac gcgctgttct tccacaatta ctcgagcttc gtcggcaagc gcgggctgta
     6121 tctggaagac gtctacgtgc aaccgggtca gcggggcagc ggtcttggca ctgcgatact
     6181 cacccggctg gcggcgctgg cagtcgagcg gcagtgcgca cgttttgaat ggaccgtgct
     6241 cgactggaat aagcaggcca ttagcttcta cgagaagatg ggcgcgacgg tgatgccgga
     6301 ttggcgtgtc gtgcgcctcg cgggtgacgc gctggaacgg ctggcaagag tggccgcttc
     6361 ctgagtttga actggctcgg cgccccctga aatcgatccg cagcgccgcg aaaaaatcag
     6421 gccgggtaac cacggtctgt cactactgct cgtcgggatc cacgcccggt agcgcatcgc
     6481 cgagcaatcc gctcgcctct tcacccggca acgcctcgac gtcacgcagc ttgcggttca
     6541 tgacgcgggt gcgggtttcc gcggcttcga tcgaacgcgt gacggtttca agctgtgcct
     6601 tggtcctggc cagcacgtcg ccgaacttgc cgaactccgt tttcaccgcg ccgagcactt
     6661 gccacacctc gctcgaccgc ctctcgatcg ccagcgtgcg gaagcccatc tgcaaactat
     6721 tgagcaaggc cgtgagcgta gtcgggccgg caatcgtcac gcggtagtcg cgttgcagct
     6781 gatcagtcag gcccggccga cgcagaactt ccgcgaacag gccttcggtc gggagaaaca
     6841 gcagcgcgaa atccgtggtg tgcggcggcg ccacatactt ctcagcgatc gtgcgtgcct
     6901 cggcgcgaat ccgcgcttcc agcgcgcgcg acgcgtcctc caccgcaacg ggatcggcgc
     6961 gctcctgagc ctcgatcagc cgctcgtaat cctcgcgcgg aaacttggcg tcgatcggca
     7021 accagaccgg cgtggcggat tcgcccgtcg ccgcgcggcc cggcagtttg atcgcgaatt
     7081 cgacgcgatc ggcgctcttc ggcaccgtag caacgttctt cgcgtattga tccgctgtga
     7141 gcaattgttc gagcaacgct tcgagttgca cttcgcccca cgtgccgcgc gttttgacgt
     7201 tggtcagaac cttcttcaga tcgccaacgc ccgcagccag tgtctgcatc tcgccgagtc
     7261 cgcgatgcac ctgctccagc cggtcggaca ccagcttgaa cgattcgccg aggcgttgtt
     7321 cgagcgtagc gtgcagtttc tcgtcgaccg tacggcgcat ctcttcgagc ctggtcgagt
     7381 tgttcgcctc gatgtctttc agccgctgct cgatggtcgc acgcacctcg gcgaagcggc
     7441 gatcgttcgc ctcggtgagc tgccccagtt gcaagctcag cgtttcgccg aactgcctga
     7501 gcgtacgccc ttgttcatcg cgtgcctgct gcgcctgctg ctgcaggctg tgacgcaccg
     7561 cgtcgagttg ctgcgcgaag ccgtcgatct tgccgccctg caccgtcgtc atactgctga
     7621 attgcgatgc cagcgtctgc tgaaagtgcg cgaatccgct gctctgctcg gtacgcgaca
     7681 cgcgcgccgt ttcggtgatg tcattgcgca gttgccgctc gagccgctca taagcatgcg
     7741 attgcgcatc tgccgctgcg tcgatacgct cgctcaaaat ctcgaaccgc tcgctttcgt
     7801 cagcgcggcc atggccgcgc atcagcaatg ccaaggcgat gaccagagcg accgccagca
     7861 cggcaacggc cgccaccaga atcatggtca tgaacgcgcc ttgccaagca cctgtgggtt
     7921 gatcggattc ggcggccggc cggcgcgcgg accttcaccc agcgccgaga tcagattgtc
     7981 cgcggcgagg ttcgccatgg cgcggcgcgt ggcttcggtc gcgctggcga tgtgcggcgt
     8041 cagtacgaca ttcggcacgc tcagcaggtc gggattcaga ttcggctcgc cctcatagac
     8101 atccagaccg gctgcggcaa tccgcttcga acgcagcgcc tcgaccagcg cggcatcgtc
     8161 gacgacgtcg cgctcgtcga ggcgttgcgc tcaaagcaga ttgcagcggc cggtctcgat
     8221 gtgttcgaag gcgagccgaa tctgaatcag gatctactca gcgtgccgaa tgtcgtgctg
     8281 acgccgcaca ttgccagcgc aaccgaagcc acgcgccgcg ccatggcgaa cctcgccgcg
     8341 gacaatctga tcgcgggcct gagtgaagga ccgcgcgcgg gccggccgcc gaatccgatc
     8401 aatcccgatg tcatcggcaa ggcgcgttca tgatcatgat tctggtggcg gccgtcgcag
     8461 tgctggcggt cgctttggtc atcgcgctgg ccttgctgat gcgcggccat agccgcgccg
     8521 aggacagcga gcagttcgac ttgctcaacg aacgcatgga tgccgcggcc gacacgcaag
     8581 cgcatgctta cgagcggctt gagcggcaac tgcgcaacga catcactgag acggcgcgcg
     8641 tgtcgcgtac cgaacagagc ggtggctttg cgcatttcca gcaaacgctg gccgcgcagt
     8701 tcagcagcat gacgacgg
//
LOCUS       DATASH010000825         5144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_336451,
            whole genome shotgun sequence.
ACCESSION   DATASH010000825 DATASH010000000
VERSION     DATASH010000825.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5144
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336451"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VHV99_22115"
     CDS             <1..210
                     /locus_tag="VHV99_22115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX3638122.1"
                     /translation="PMHVQKSADGYLDMIRRGGFRFEQRNVSLPYLWWSRAKDFGLLE
                     RIGLTHPQPGKRRETLVNVAAVKTD"
     gene            complement(295..738)
                     /locus_tag="VHV99_22120"
     CDS             complement(295..738)
                     /locus_tag="VHV99_22120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HEX3638123.1"
                     /translation="MKRNVLFAALFASLTMLSATQAFARDTIANYPVDAALQSEPGKV
                     SDDVKLYFAGQSHPAVLKTMGNFATNKKTNAFGKSDLQACQHVFLSAVIELQDRARKE
                     GGNAVINIKSNYKNEVRESATEFTCGAGAVVAGVALTGDVVTLRQ"
     gene            complement(874..2103)
                     /locus_tag="VHV99_22125"
     CDS             complement(874..2103)
                     /locus_tag="VHV99_22125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX3638124.1"
                     /translation="MSMALQRVVVTGMGIVSCLGNTLDEVAAALRAGRSRIERIEAWR
                     ERGFGSQVAGVASVAEEQPFERKLERFMGDTARFACHAAKKAIDDAGLGPAALRSPQA
                     GTVIGSGVGTMSTYDVAMSIANARGVDKVPPYTVPHAMSSTASANVAQVFGLEGVSYS
                     PSSACTTSALAIGQAMQLIQTGRQQIVLAGGSESLHDNMTLMFDSMGALSRGFNDTPQ
                     RASRPYALARDGFVIASGGGVLVLESLDHALARGARIYAELIGFGDCTDSAGMVTPRA
                     AGIARAMRGALGEARRRPDYINTHAPSTPLGDVEELRALVDVFGGEIPAFSSTKGMTG
                     HPLGACGAHEAIYTLLMMRDGFIAGTPEIEAAEPAVDGMPLVRTTRDACIGTAMSISY
                     GFGGSCASLMFAMWQGG"
     gene            complement(2100..3326)
                     /locus_tag="VHV99_22130"
     CDS             complement(2100..3326)
                     /locus_tag="VHV99_22130"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HEX3638125.1"
                     /translation="MKRVVITGMGGVTAFGDDWDAIEARLRSGVNAVRRMSEWDYFES
                     LHTRLACPLPGFTMPAHYPRKKTRSMGPVSMYSVRASELALADAGLADDLSIKDGRMG
                     VAYGSSSGSVQPIRAFGTMLETGSMSDVTSNSYVQMMPHTTAVNVSLFWDLKGRIIPT
                     SCACASGSQAIGYAYEAIQTGKQTLMLAGGAEEMSGPAVAVFDTLYATSTRNDEPRLT
                     PRPFDAARDGLVVGEGAATLVLEEYEHAVARGARIHAEIVGFGCNSDGAHMTQPTAET
                     MALAMQLALQDAQLAPEAIAYVNAHGTSTDRGDVAESHATAQTFGARMPISSLKSYVG
                     HTLGACGALEAWWTIEMMKRNWYAPTLNLENVDPACAPLDYIVGAGREIDADYVMSNN
                     FAFGGINTSLIFRRVR"
     gene            complement(3326..4051)
                     /locus_tag="VHV99_22135"
     CDS             complement(3326..4051)
                     /locus_tag="VHV99_22135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase FabG2"
                     /protein_id="HEX3638126.1"
                     /translation="MSRRVLVTGASRGIGRAIAYQLAADGFAVSVHCRTGRTEAEAVA
                     TGIAAQGGTARVLQFDVRERAVCREVLEADVAAHGPYYGIVCSAGVTRDAAFPALTEE
                     DWDIVIETGLDSFYNVVHPLTMPMVRAKKGGRIVTIASVSGVMGNRGQVNYSAAKAGL
                     IGASKALAVELATRNITVNCVAPGLIETGMLEEMPLEHALKTVPMNRVGQPAEVASVV
                     SFLMSDAASYVTRQVIGVNGGMV"
     gene            complement(4048..4533)
                     /locus_tag="VHV99_22140"
     CDS             complement(4048..4533)
                     /locus_tag="VHV99_22140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog family protein"
                     /protein_id="HEX3638127.1"
                     /translation="MTHTLSTEELVQQPIEAIIPHRGTMLLLDAVNTFADDTLSARAT
                     VHADAWYADDDGAMPAWIGIELMAQAIAAHVALLAMRGGGSARPGVLLGSRSYKALQP
                     SFASGAQLLINATELLRSEEGHGAYECTIHHGDVCCAEAVIKVFQPRDFQSFIEGSFS
                     S"
     gene            complement(4542..>5144)
                     /locus_tag="VHV99_22145"
     CDS             complement(4542..>5144)
                     /locus_tag="VHV99_22145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HEX3638128.1"
                     /translation="QGFASLESTSNVRSNPMSRNRCGINVGEGAAVFLMSREEAAVRL
                     AGVGESSDAHHISSPDPQGVGGELALRAALADAGVASAAIGYVNLHATATRKNDEMEA
                     KLMARVFPGCVATSGTKPLTGHQLGAAGATELAFAWLTLARDDVPLPRHLWDGDADPA
                     LPVLDLVESERFLPRGEGSQYVMSNSFAFGGSNVSLILGR"
BASE COUNT          887 a         1759 c         1574 g          924 t
ORIGIN      
        1 cccatgcacg tgcagaaaag cgccgacggc tatctcgaca tgatccgccg cggcggtttc
       61 cgcttcgagc agcgcaacgt ctcgctgccg tatctctggt ggagccgcgc gaaagatttc
      121 ggcctgctcg aacgcatcgg cctgacccat ccgcaacctg gcaagcgtcg tgaaacgctg
      181 gtaaatgtcg cggcggtcaa gacagactga atcttgccga ggcggtggtg caccaccgcc
      241 tcggcatcaa cgcgcgctta tttgggtgct gatttgcgta aactgcgtgc gcgcttactg
      301 acgcagcgtc accacatcgc ccgtcaacgc cacaccggcg accaccgcgc ccgcaccgca
      361 ggtgaactcc gtcgcgctct cacgcacttc gttcttgtag ttgctcttga tgttgatcac
      421 cgcgttgccg ccttccttgc gcgcgcgatc ctgcaattcg atcacggccg acaggaacac
      481 gtgctggcac gcttgcagat cgctcttgcc gaaggcgttg gtctttttgt tggtcgcgaa
      541 attgcccatc gtcttcagca cggccggatg cgactggccc gcgaaataca gcttgacgtc
      601 gtcactgacc ttgcccggtt cgctttgcag cgccgcatcg accggatagt tggcgatcgt
      661 gtcacgggcg aaagcttgag tagcgcttag catggtgagc gaagcgaaca gtgcggcaaa
      721 cagaacattg cgtttcatag aaactcctta gtggtttttg ttgctctggt gggttctaaa
      781 tctgattcga ctcaatttac ttccagcacg acgagctggc cgctcactgc gattgcggga
      841 gcgctagtgc ccgttcttta aatgacgacc gcgtcaaccg ccctgccaca tggcaaacat
      901 caggcttgcg caactgccgc cgaacccgta tgaaatcgac attgccgtgc cgatgcacgc
      961 gtcacgcgtc gtacgcacga gcggcatgcc gtcgacggcc ggctccgccg cctcgatctc
     1021 cggcgtcccc gcgataaaac cgtcacgcat catcagcaac gtgtagatcg cctcgtgcgc
     1081 gccgcaggcg ccgagcggat gcccggtcat gcctttggtc gacgagaacg cggggatttc
     1141 gccgccaaat acatcgacca aagcacgcaa ctcctcgaca tcgccaagcg gcgtcgacgg
     1201 tgcgtgggtg ttgatatagt ccggccgcct gcgcgcttcg cccagcgcgc cgcgcatcgc
     1261 tcgcgcgatg ccggccgcgc gcggcgtcac catgcccgcg ctgtccgtgc agtcgccgaa
     1321 gccgatcagc tcggcgtaaa tgcgggcccc gcgcgccagc gcgtgatcga gcgattcgag
     1381 caccagcacg cccccgcccg aggcgatcac gaagccgtcg cgcgccaaag cgtagggacg
     1441 cgaagcgcgc tgcggcgtgt cgttgaagcc gcgcgacagc gcccccatcg agtcgaacat
     1501 cagcgtcatg ttgtcgtgca gcgactcgct gccgcctgcc agtacgatct gctgacggcc
     1561 ggtttgaatc agctgcatcg cctggccgat ggcgagcgcc gacgtcgtgc acgccgacga
     1621 cggcgaatag ctcacacctt cgagtccgaa cacctgcgcg acgttggcgg acgcggtgct
     1681 gctcatcgca tgcggcaccg tgtacggcgg cactttatcc acgccgcggg cgttcgcaat
     1741 cgacatcgcc acatcgtaag tcgacatcgt gccgacgccc gagccgatca ccgtaccggc
     1801 ctgcggcgaa cgcaaggcgg cggggccgag cccggcatcg tcgatcgctt tcttcgcggc
     1861 gtggcacgcg aagcgcgcgg tgtcgcccat gaagcgttcg agcttgcgct cgaacggctg
     1921 ctcctctgca accgacgcga cgcccgccac ctgcgatccg aagccacgct cacgccacgc
     1981 ttcgatccgc tcgatgcgcg agcggcccgc gcgcaacgcg gcggccactt cgtcgagcgt
     2041 attgccgagg cacgacacga ttcccatgcc ggtgacgacc acccgttgca gcgccatgct
     2101 catcgaacgc gcctgaaaat cagcgacgtg ttgatgccgc cgaatgcaaa gttgttgctc
     2161 atcacatagt cggcatcgat ctcgcggccc gcgcccacga tgtaatcgag cggtgcgcag
     2221 gccggatcga cgttctccag attcagggtc ggcgcgtacc agttgcgctt catcatctcg
     2281 atggtccacc acgcttccag cgcaccgcat gcacccagcg tatggcccac gtagctcttg
     2341 agcgaactga tcggcatgcg cgcgccgaag gtctgcgcgg tcgcatggct ttcggcgacg
     2401 tcgccacggt ctgtcgacgt gccgtgcgcg ttcacatagg caatcgcttc gggtgccagt
     2461 tgcgcgtctt gcaaagcgag ctgcattgcc aacgccatgg tttcggcggt cggctgggtc
     2521 atgtgcgcgc cgtccgaatt gcagccgaag ccgacgatct ccgcatgaat ccgcgcgccg
     2581 cgcgccaccg cgtgttcgta ctcctcgagc accagggtgg ccgcgccttc gccgaccacg
     2641 aggccgtcgc gcgcggcatc gaatggacgc ggcgtcagac gcggctcgtc gttgcgtgtg
     2701 ctggtcgcgt acagcgtgtc gaacacggcg accgccgggc ccgacatttc ttccgcgccg
     2761 cccgccagca tcagcgtctg cttgccggtc tggatcgcct catacgcgta gccgatggcc
     2821 tggctgcccg atgcgcatgc gcacgaggtc ggaatgatcc gccctttcag atcccagaac
     2881 aggctgacgt tgaccgccgt ggtatgcggc atcatctgca cgtaactgtt cgacgtgacg
     2941 tcgctcatcg agccggtttc gagcatcgtg ccgaacgcgc gaatcggctg caccgagccc
     3001 gacgacgaac cgtaggcgac gcccatgcgg ccgtccttga tgctgaggtc gtcggcgagg
     3061 cccgcatcgg cgagcgccag ttcgctcgcg cgcaccgaat acatcgacac gggtcccatc
     3121 gagcgggttt tcttgcgcgg gtaatgcgcg ggcatcgtga atcccggcaa cggacatgcg
     3181 agccgcgtat gcagcgactc gaagtaatcc cattcggaca tccggcgcac cgcgttcacg
     3241 ccgctcctca ggcgcgcttc gattgcgtcc cagtcgtcac cgaacgcggt gacgcccccc
     3301 atgccggtaa tgacgacgcg tttcatcaca ccatcccacc attgacgccg atcacctgac
     3361 gcgtgacata cgaggccgca tccgacatca ggaagctgac cacggacgcc acttcggcag
     3421 gctggccgac gcggttcatc ggcaccgtct tcaatgcgtg ctcgagcggc atctcctcga
     3481 gcatgccggt ttcgatcaaa cccggcgcga cgcaattgac ggtgatgttg cgcgtcgcca
     3541 gttcgacggc gagcgccttg ctcgcgccga tcaggccggc tttcgccgcg ctgtagttga
     3601 cctggccgcg attgcccatc acgccggaga ccgacgcgat ggtgacgatg cgcccgccct
     3661 tcttcgcgcg caccatcggc atggtcaatg gatggacgac gttgtagaac gaatcgagac
     3721 cggtttcgat cacgatgtcc cagtcttcct cggtgagcgc ggggaacgcc gcgtcgcggg
     3781 tcacgcccgc gctgcacacg atgccgtagt acgggccgtg cgccgcgaca tccgcttcga
     3841 gcacttcgcg gcacacggcg cgctcacgca cgtcgaactg cagcacgcgc gccgtgccgc
     3901 cttgcgcggc gatacccgtg gcgaccgctt cggcttcggt gcggccggtg cggcaatgca
     3961 cggacaccgc gaagccgtcg gcggccaact ggtacgcaat cgcgcggcca atgccgcggc
     4021 ttgcgccggt aacgagaaca cgccggctca tgaactgaaa ctcccttcaa tgaatgactg
     4081 aaaatcacgc ggctgaaaga ccttgatgac cgcctcggcg cagcacacgt caccgtggtg
     4141 aatcgtgcat tcgtaggcgc cgtggccttc ttcgctacgc agcaattcgg ttgcgttgat
     4201 cagcaattgc gcgccgctcg cgaacgacgg ttgcaaagcc ttataactgc gtgagccaag
     4261 cagcacgccg ggacgcgcgc tgccgccgcc gcgcatcgcc agcaacgcga cgtgcgcggc
     4321 gatcgcctgc gccatcaatt cgataccgat ccaggcgggc atcgcgccat cgtcgtcggc
     4381 gtaccaggcg tcggcgtgga ccgttgctcg cgcgctgagt gtgtcgtccg cgaacgtgtt
     4441 taccgcgtcg agcagcagca tggtgccgcg atgcgggatg atcgcctcga tcggttgctg
     4501 gacgagttcc tcggtggata gcgtatgcgt catcgcgcgc gtcaccgtcc aaggatcagg
     4561 ctgacattgc tgccgccgaa cgcaaacgaa ttgctcatca cgtactgcga gccttcgccg
     4621 cgcggcagaa aacgctcgct ctcgaccaga tccagcaccg gcaaagcggg atcggcatcg
     4681 ccgtcccata gatgacgcgg caacggcacg tcgtcgcgcg cgagcgtcag ccatgcgaag
     4741 gcgagttcgg tggcgcccgc cgcgcccaac tggtgaccgg tcagcggctt ggtgccgctc
     4801 gtcgccacgc agccggggaa cactcgcgcc atcagcttcg cttccatctc gtcgttcttg
     4861 cgcgttgctg tggcatgcag attcacatag ccgatcgccg cggacgcgac gcccgcatcg
     4921 gccagcgccg cgcgcaatgc aagctcgccg cccacccctt gcggatccgg cgaggaaata
     4981 tgatgggcgt cgctcgattc gccgaccccc gcgagtctca ccgccgcttc ttcgcggctc
     5041 atcaggaaca cggcggcgcc ttcgccgacg ttgatcccgc aacgattgcg gctcattgga
     5101 ttgctgcgca cgttgctggt cgattcgagc gatgcgaagc cttg
//
LOCUS       DATASH010000826         3134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3501676,
            whole genome shotgun sequence.
ACCESSION   DATASH010000826 DATASH010000000
VERSION     DATASH010000826.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3501676"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..1218)
                     /locus_tag="VHV99_22150"
     CDS             complement(43..1218)
                     /locus_tag="VHV99_22150"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEX3638129.1"
                     /translation="MTSERAKRFPLGLEDLVREPRRPEGRPGEVRAAALAAAAALNAR
                     PQPAKNSPPGVAGKVQAKPPARAQSQPQATSQARPQARPQAKPSARAQSQPQVTSQTK
                     PQAKLPMKAPASSSPSSSVSTQPKPQTQQHAKPPAPSVAQGSAVRAAPKPAPVHQPAV
                     KLNERTAVPNVALNGALALTSERVRERMVERLRANGVTDQRVLNAMSVVPRHMFVDPG
                     LAAQAYEDAALPIGHHQTISKPSVVARMIELAAAGRALNTVLEIGTGCGYQAAVLSQV
                     ARDVYSIERIKPLSERAKTNLRPLRIPNIRLHYGDGRLGLPSAAPFDAIVIAAAGLDV
                     PQALLEQLAIGGRLVAPVGSQEGQNQVLTLVERLGPAQWRESRLDRVFFVPLKSGVI"
     gene            complement(1215..1973)
                     /gene="surE"
                     /locus_tag="VHV99_22155"
     CDS             complement(1215..1973)
                     /gene="surE"
                     /locus_tag="VHV99_22155"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEX3638130.1"
                     /translation="MRILLSNDDGYLAPGLAALYEALKPIADVTVMAPEQNCSGASNS
                     LTLSRPLSVLRSANGFYYVNGTPTDSVHIALTGMLDHTPDLVVSGINNGQNMGEDTLY
                     SGTVAAATEGIMFGVPAIAFSLVDKDWVHLEDAARVAAEIVAHYLEQPLPGHPLLNVN
                     IPNLPYEQLNGWHITRLGKRHPSQPVIRQTNPRGEPIYWIGPSGSARDASEGTDFHAV
                     ANGQVSITPLQLDLTHTQMLPAARDWARAGSGAS"
     gene            2198..>3134
                     /locus_tag="VHV99_22160"
     CDS             2198..>3134
                     /locus_tag="VHV99_22160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HEX3638131.1"
                     /translation="MRAIRCNQYGPPESLVVENLPDLEPPPGHVVIDVKAAAVNFPDV
                     LIIENKYQFKPPLPFTPGSEVAGIVRAVGADVTQFKPGSRVVAYTGTGGFAEQALAPV
                     AACVPLADGVDFELAAAFTLAYGTSHHAVVDRGQLKAGETMLVLGAAGGVGLAAVEIG
                     KALGARVIAAASSDEKLATCVKHGADATINYSSEDLRERIKALTAGQGPDVIYDPVGG
                     MYAEPAFRSIGWRGRYLVVGFANGEIPKLPLNLTLLKGASLVGVFWGDFAKREPQHNQ
                     AAFQQMTGWIAEGKLKPYVSARYALDDTGRALRDMA"
BASE COUNT          527 a          983 c         1064 g          560 t
ORIGIN      
        1 tcagtctggt tctttgcatc gcgcgcaaca tactcatcgg tgtcaaatca ctccggattt
       61 taagggtaca aagaaaacgc gatcaagccg cgactcgcgc cactgcgcgg gtccgaggcg
      121 ctcgaccaga gtcagcacct ggttctggcc ttcctgcgag ccgaccggcg cgaccagacg
      181 gccgccgatc gcgagttgtt caagtaatgc ttgcggtaca tcgaggccag ccgcggcgat
      241 cacgatcgca tcgaacggcg ccgccgacgg cagccccagg cggccatcgc cgtagtgcag
      301 ccggatgttc ggaatgcgca gcggacgcaa gttcgtcttc gcgcgttcgg acagcggctt
      361 gatgcgttcg atcgaataaa cgtcacgcgc gacctggctc agcactgccg cctgatagcc
      421 gcaaccggta ccgatttcga gcacggtgtt caacgcgcgg ccggcggccg ccagttcgat
      481 catccgggcg accacggagg gtttggagat cgtctggtga tggccgatcg gcaacgcggc
      541 atcttcatag gcctgggccg cgagacccgg gtcgacgaac atgtgacgcg gcaccaccga
      601 catcgcgttc aacacgcgct gatcggtcac accgttcgca cgcaggcgtt cgaccatccg
      661 ttcacgaacc cgttccgaag tcagcgccaa agcgccgttc aacgcgacgt tcggcacggc
      721 ggtacgctcg ttgagtttga cggcgggctg gtgcaccggc gccggttttg gcgcggcgcg
      781 cacagccgaa ccttgcgcga cggatggtgc gggtggtttg gcgtgttgtt gggtttgcgg
      841 cttgggctgg gtgctaaccg atgagctcgg tgacgaactc gccggtgcct tcattggcag
      901 cttcgcttgc ggctttgttt gcgacgttac ttgcggttgg ctctgcgccc tcgccgacgg
      961 cttcgcttgc ggccttgctt gcggccttgc ttgcgacgtg gcttgcggtt ggctctgcgc
     1021 cctcgccggc ggcttcgcct ggacttttcc cgcgacgccg ggcggcgagt tcttcgccgg
     1081 ctgcgggcgc gcgttcaacg cggcagccgc ggcgagtgcc gccgcgcgca cttcgcccgg
     1141 acgcccttca ggccggcgcg gttcgcgcac cagatcctcg aggccgagcg gaaagcgctt
     1201 tgcgcgctcg ctggtcatga agcgccgctg ccggcgcgcg cccagtcgcg cgctgcgggc
     1261 agcatttgcg tgtgggtcag gtcgagctgc aacggcgtaa tcgacacctg tccgttcgcg
     1321 acagcgtgga aatcggtgcc ttcgctcgca tcgcgcgcgc tgcccgacgg cccgatccag
     1381 tagatcggtt cgccgcgcgg gttggtctgg cggatcaccg gctgcgacgg atgccgcttg
     1441 ccgagacgcg tgatatgcca gccattgagt tgctcgtacg gcaggttcgg aatattgacg
     1501 ttcagaagcg gatgccccgg caacggctgc tcgaggtagt gcgcgacgat ctcggcagcg
     1561 acgcgcgcgg cgtcttcgag atgcacccag tctttgtcga ccagcgagaa ggcgatggcc
     1621 ggcacgccga acatgatgcc ctcggtggcg gcggcgacgg tgcccgaata cagcgtgtcc
     1681 tcgcccatgt tctggccgtt gttgatgccc gagacgacga ggtccggcgt gtggtccagc
     1741 ataccggtca acgcaatatg caccgagtcg gttggcgtgc cgttgacgta gtagaaaccg
     1801 ttcgccgagc gcagcacgga gagcggccgc gacaacgtca aggagttcga cgcgccgctg
     1861 cagttctgct cgggggccat cacggtgacg tccgcgatcg gtttgagcgc ttcgtaaagc
     1921 gcggcaaggc cgggcgccag atagccgtcg tcattgctga gtaggattcg catgcggcga
     1981 ttgtaaccga ggaaacaagg cacgcgaacg acacggcaga cgctgcgcgc atcacatgca
     2041 agcgcgcgca cgcggcgccg cgccaacggg tgcggacagc gcaagcatcc tgctccgggc
     2101 aatgaaaaag cacggtcgtg ctgattgctt tacactcaaa atggttgcga tgccccgaag
     2161 gacgtccctt gggggacctg atcgatatgg agacacgatg cgcgcgattc gctgtaatca
     2221 atacgggccg ccggaaagcc tggtcgtcga aaatctgccg gatctcgagc cgccgcccgg
     2281 ccacgtcgtg atcgacgtca aagccgcggc cgtcaatttt cccgatgtgc tgatcatcga
     2341 gaacaaatac cagttcaaac cacccctgcc ctttacgccg ggttcggaag tcgcgggcat
     2401 cgtgcgcgcg gtcggcgccg atgtgacgca gttcaagccg ggctcgcggg tggtcgcata
     2461 caccggcacg ggcgggtttg ccgagcaggc gctcgcgccg gtcgcggcgt gcgtgccgct
     2521 ggcggacggc gtcgacttcg aactggccgc cgcgttcacg ctcgcctatg gcacgtcgca
     2581 tcatgcagtg gtcgatcgcg gccagttgaa agccggtgag acgatgctgg tgttgggcgc
     2641 ggcaggcggc gtcgggctcg ccgcggtcga gatcggcaag gcactcggcg cgcgcgtgat
     2701 cgcggcggcg tcgagcgacg agaagctcgc cacctgcgtg aaacacggcg cggacgccac
     2761 catcaactac agcagcgaag atctacgcga gcgcatcaag gcgctgaccg ccggccaggg
     2821 tccggacgtg atctacgatc cggtcggcgg catgtatgcg gaaccggcgt ttcgcagcat
     2881 cggctggcgc ggacgttatc tggtggtcgg ctttgcaaac ggtgagattc cgaagttgcc
     2941 gctcaacctg acgctgctca aaggcgcgag tctggtcggc gtattctggg gcgacttcgc
     3001 caaacgcgag ccacagcaca atcaggctgc gttccagcag atgacgggat ggatcgccga
     3061 aggcaagctc aagccgtacg tgtccgcacg ttatgcgctc gacgacacgg gccgcgcgtt
     3121 gcgcgatatg gctg
//
LOCUS       DATASH010000827         1665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5282269,
            whole genome shotgun sequence.
ACCESSION   DATASH010000827 DATASH010000000
VERSION     DATASH010000827.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5282269"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..823
                     /locus_tag="VHV99_22165"
     CDS             53..823
                     /locus_tag="VHV99_22165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3638132.1"
                     /translation="MDLKLKGLKALVTGGTKGIGRAIAQTLAAEGADVALCARDGATV
                     EQTVADIVQVTGGAVKVCGASVDVSNGTALQAWTREAAAQLGGIDIVVANVSALSIGN
                     DLESWRKEFETDLMGTVNLIDAAMPFLEASKAASIIAISSVSGREIDFAAGPYGTFKA
                     ALIHYMQGLAYHLAPKGIRANAVSPGNVYFEGGVWDHIEHNDPKLFAEALALNPSGRM
                     ARPQEIANAVAFVASPAASFVSGANLVVDGALTRGVQL"
BASE COUNT          323 a          512 c          512 g          318 t
ORIGIN      
        1 cgcggcgatc agcaagaaat atcttgcacc ggctaactaa ggggacgcca ccatggactt
       61 gaaactcaaa ggtttgaagg cactcgtaac cggcggcacc aagggtatcg gccgcgccat
      121 tgcgcaaacg ctggcagccg aaggcgcgga cgtcgcgctt tgcgcccgtg acggtgccac
      181 tgtggaacag acggtggccg acatcgtgca agtcaccggt ggcgcggtca aggtgtgcgg
      241 cgcctcggtc gacgtttcga atggtacggc gttacaggcc tggacccgcg aggccgccgc
      301 gcaactgggc ggcatcgaca tcgtggtcgc caatgtgagc gcgctgtcga tcggcaacga
      361 tctcgaatcg tggcgcaagg agttcgaaac cgacttgatg ggtacggtca acctgatcga
      421 cgccgcgatg ccgtttctcg aagcgagcaa ggccgcctcg atcatcgcga tatcgagcgt
      481 gtccggccgt gaaatcgact ttgccgccgg cccttacggg accttcaagg cggcgctgat
      541 tcactacatg caaggcctcg cgtaccacct cgcgccgaag ggcatccgag cgaacgccgt
      601 atcgccgggc aacgtctatt tcgaaggcgg tgtgtgggat cacatcgaac ataacgatcc
      661 gaagctgttc gcggaagcac tcgcgttgaa tcccagcgga cgcatggcgc gcccacagga
      721 gatcgcgaat gcggtcgcgt tcgttgcgag tccggcggcg agcttcgtga gcggggcgaa
      781 tcttgtagtg gatggcgcgc tcacgcgcgg ggtgcagctc taagtgcctg gagctgcatc
      841 acttaggtcg attcgacaag aataaagcgc ccggccgttt cttggacgcg gcctgattgt
      901 cgacccgcaa ggttggccgg cggccggtcc tcccggtcca acggacgaag aaattctgac
      961 cgcgtcggtg aatctggccg acgcgcgtcg gaaccgccaa ttgaacgcgt ttgctcacgt
     1021 gttgcgtgac cgccgtccag atatctactg aaacgggccg tcaatcgtaa gcgttcaccc
     1081 ccctgcgcta tacgtccgta taggcggagt acgctaacgc ggcattggca tatccgaccg
     1141 tagagtagat tcgccctctg gctcgactta atcttctctc atcgctacac gctcgctgtt
     1201 tcaccagcga gaaaaagctc gcggttcgcc ccccgacgac atggcgctcc cggatgcccg
     1261 ccggccgatt tcgccttcgc aatgattctg gccagtgagc cgatcaaggc ccacacaagc
     1321 gcctgagacg tgctggcgta ggcggtacgc tcttcggtct ggctcccgca gcctttacat
     1381 gcaactagag gtcgcgaggt tttgcgcgac tgcaccgtct tcctccgggc ctcgacgatg
     1441 tattcaaccc aggcgaggtc gcgcccgcag acgaggctgc gagtctcgct tcactggccc
     1501 tgcgacgctc actgctgaat gccgatccct gcgtcatatt gccagggcgc cggaacgaac
     1561 cgcagacacc cgtcgtgagt gcttacatgc cgaggcgaaa ccatgaagtg gactgcggat
     1621 accttagcaa tcttgcgcga tttgcactcg ctgaaagtag ttgtg
//
LOCUS       DATASH010000828         2337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2317659,
            whole genome shotgun sequence.
ACCESSION   DATASH010000828 DATASH010000000
VERSION     DATASH010000828.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2317659"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..1142
                     /locus_tag="VHV99_22170"
     CDS             159..1142
                     /locus_tag="VHV99_22170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3638133.1"
                     /translation="MDNLGDIRLFVEAAQQGSLSAAGRKMGLTPAAASARLAKLEAGL
                     KARLFERTTRQLRLTDEGRLYLNCCRQALQSLDDAEAALQAGQGVVRGKVRISATSDF
                     GRNLLMHWLDEFNVLYPQVTFALTLSDSLSNLVQQDIDLAIRFGVPQDSSLVARMLAP
                     NRRVLCASPDYIARKGEPKDPQDLANFDCIVLGTASGPVNEWRFTRGDEVQHYTVPLE
                     TSRETNDGAVAREWARRGYGIVIKSMWDVEADLRADCLKILLPEWRYPDAPLHALYHR
                     NRFMAPRVRVLLDFLSERFAHVSDELEGLLGLPPEQPRTIAAAETASYEGL"
     gene            1369..>2337
                     /gene="miaB"
                     /locus_tag="VHV99_22175"
     CDS             1369..>2337
                     /gene="miaB"
                     /locus_tag="VHV99_22175"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEX3638134.1"
                     /translation="MTKKVYVKTFGCQMNEYDSDKMVDVLGAADGLVKTDTPEDADVI
                     LFNTCSVREKAQEKVFSDLGRVRELKEANPNLIIGVGGCVASQEGASIVARAPYVDLV
                     FGPQTLHRLPQMIDKRRESGRAQVDISFPEIEKFDHLPPARVDGPTAFVSIMEGCSKY
                     CSYCVVPYTRGEEVSRPLDDVLTEIAGLADQGVREVTLLGQNVNAYRGPLTQHSTDIA
                     DFATLIEYVADIPGIERIRYTTSHPKEFTQRLIDTYAKVPKLVSHLHLPVQHGSDRIL
                     MAMKRGYTVLEYKSVIRKLRAIRPDLSLSTDMIVGFPGETEEDFDKM"
BASE COUNT          469 a          717 c          724 g          427 t
ORIGIN      
        1 tcgagctcgg cggtgacgat cgttgcgtga tcgagttgca tagcgtgggc tgcgcgggag
       61 cacaaagggt ttacgaggac tgcattgtga agtggacgcg tcaatttgat aattggcgta
      121 gcatttgaag cattttcaaa ttattttgaa agattgagat ggataacctc ggtgacattc
      181 gcctgttcgt cgaagccgcg cagcagggca gcctctccgc ggcgggccgc aagatgggcc
      241 tgacgccggc cgccgccagc gcgcggctgg ccaaactcga agccgggctg aaggcgcgtc
      301 tgttcgaacg cactacccgt cagttgcggc tcacggacga aggccgcctc tacctgaact
      361 gctgccgcca ggcgctgcaa tcgctcgacg acgccgaagc cgcgctgcaa gcgggccagg
      421 gcgtggtgcg cggcaaagtc cgaatttcgg caacttcgga tttcggccgc aacctgctga
      481 tgcactggct cgacgaattc aacgtgcttt acccgcaggt gacgttcgcg ctgacgctgt
      541 cggactcgct atcgaatctg gtgcagcagg acatcgatct ggcgatccgc ttcggcgtgc
      601 cgcaggacag ctcgctggtc gcccgcatgc tggcgccgaa ccggcgcgtg ctgtgcgcgt
      661 cgccggacta catcgcccgc aagggtgagc cgaaagatcc gcaagatctg gcaaatttcg
      721 actgcatcgt gctcggcacc gcgtccgggc cggtcaacga atggcgcttc acgcgcggcg
      781 acgaagtgca gcactacacg gtgccgctcg agacctcccg ggaaaccaat gacggcgccg
      841 tggcccgcga atgggcgcgg cgcggctacg ggatcgtcat caagtcgatg tgggacgtgg
      901 aagcggactt gcgcgccgac tgcctgaaaa tcctgctgcc cgagtggcgc tatccggatg
      961 cgccattgca cgcgctttac caccgcaacc ggttcatggc gccgcgcgtg cgggtcctgc
     1021 tggattttct gagcgagcgc ttcgcgcacg tgtcggacga actggaaggc ctgctgggcc
     1081 tgccgccgga gcagccgcgg acaatcgccg cggcagaaac tgcatcctat gaagggctat
     1141 aatactgagc tacgctgcaa acgtgcattg aactggagcc tgactagccc gaaccgggtc
     1201 gaaaccgggc tctaacggtg cctgggaggt gcctggcagg tacctaaacg ggtagcgagc
     1261 gttcaagcgg ctgcccaagc gccgccccac ggcccgtccg cacgttttca tccctcttca
     1321 ccttttaaac gacgccccca ggttaaccac tgcgaccggc gaaattcgat gaccaagaaa
     1381 gtttatgtaa aaacctttgg ctgccagatg aatgagtacg actccgacaa gatggtcgac
     1441 gtactcggcg cggccgacgg actcgtcaag accgacacgc cggaagatgc ggacgtcatt
     1501 ctcttcaaca cctgttcggt gcgcgaaaaa gcccaggaaa aagttttctc cgacctcggc
     1561 cgcgtgcgcg agttgaaaga agcgaatccc aatctgatca tcggcgtggg cggctgcgtg
     1621 gcgagccagg aaggcgcgtc gatcgtggcg cgcgcgccgt atgtcgacct ggtgttcggt
     1681 ccgcaaacgc tgcaccgtct gccgcaaatg atcgacaagc gccgtgaaag cggccgcgcc
     1741 caggtcgata tctcgttccc ggaaatcgaa aagttcgacc atttgccgcc ggcgcgcgtc
     1801 gacggcccga ctgcgttcgt gtcgatcatg gaaggctgca gcaagtactg cagctattgc
     1861 gtcgtgccgt acacacgcgg tgaagaagtg tcgcggccgc tcgacgacgt gctgaccgaa
     1921 atcgccggcc tcgccgacca gggcgtgcgc gaagtcaccc tgctcgggca gaacgtgaat
     1981 gcgtaccgtg gaccgttgac acagcattcc accgatatcg ccgatttcgc cacgctgatc
     2041 gaatatgtcg cggacattcc gggcatcgaa cggattcgct acaccacgtc gcatccgaaa
     2101 gaattcacgc agcgcctgat cgacacctac gcgaaggtgc ccaagctcgt gagccacttg
     2161 cacttgcccg tgcagcacgg ttccgaccgt attctcatgg ccatgaaacg cggttacacc
     2221 gtgctcgaat acaagtcggt gatccgcaag ctgcgcgcga tccgtccgga tctgtctttg
     2281 tccacggata tgatcgtcgg cttccccggc gagacggagg aagacttcga caagatg
//
LOCUS       DATASH010000829         6115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4493691,
            whole genome shotgun sequence.
ACCESSION   DATASH010000829 DATASH010000000
VERSION     DATASH010000829.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6115
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4493691"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2132..2569
                     /locus_tag="VHV99_22180"
     CDS             2132..2569
                     /locus_tag="VHV99_22180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGDYG domain-containing protein"
                     /protein_id="HEX3638135.1"
                     /translation="MTELKNLDLRTDAAARRVVKNETVTVEFAATEGELMSLEGPNRY
                     ARGDALITGSTGDRWVVSRERFDAKYLPEDSTRAHGEPGAYRNRPAVVLAKQMNEAFS
                     LARSASGGDVLHGAAGDWIMQYAPGDYGVVQAARFAKVYKLAD"
     gene            complement(2714..3079)
                     /locus_tag="VHV99_22185"
     CDS             complement(2714..3079)
                     /locus_tag="VHV99_22185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3022 domain-containing protein"
                     /protein_id="HEX3638136.1"
                     /translation="MEAYQYDCANPEFEELARVISDLFPEQTQFIQRAGEDGTPTLAI
                     HWVAMRFGAAARRIVMAVVITPAALARYRAMPARLRGRSFAVLRAYVEASIGSLEEQY
                     ANGETVPREVTVDLGDEFA"
     gene            complement(3168..3980)
                     /locus_tag="VHV99_22190"
     CDS             complement(3168..3980)
                     /locus_tag="VHV99_22190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HEX3638137.1"
                     /translation="MVVAPLTLSSLAVATLFVAALPFLIYRRLRRTLALNPRDAITGI
                     AIFALFAMVIERALNDYLLHRNEATATFLSNPLAFVVYGALVAGVCEEVGRFIGMRLL
                     LKRAAAKSASGVAGKGDGTALTYGLGHGGAEAWLVGVLVQIQWILFAVFENRGELDGY
                     LSNLPTDSVMRIHLILASLTPQTAGIFALERVAALVFQIGLSVLMWRGVRAGWRGILP
                     LAIVLHALVDVPAALFQAQLVPLVAVDGAYAVGAVIVAGVLFSVFRRPAVAA"
     gene            complement(4053..5246)
                     /locus_tag="VHV99_22195"
     CDS             complement(4053..5246)
                     /locus_tag="VHV99_22195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX3638138.1"
                     /translation="MSTTPLAAPRRATITDVAREAGTGKTSISRYLNGEMSVLSPELR
                     ARIEAAIERLDYQPNQMARGLKRGRNRLIGMLLADLTNPYTVEVLQGVEAACHALGLM
                     PLICHAANEVEMERRYLQLLTTYRVEGVIVNALGVREETLRPVGGGGIPAVLVDRSVE
                     GLVADMVGLDNRAAAELGTRHLLESGFDDIWFVVQPFEQVSSRQLREAAFREVMRGEG
                     GGGHAGRTSGKSEGSKGGKGGARGHTLVLNLAEPAAVERALAELDCALDAAGGPRGAL
                     ASGADNAARISTGDAAGNATTTPPSKPSRIALFAANAPVALCLALHLKARYGADWQTH
                     VALLSIDDPEWAELTGVTTIRQPTYEIGYRAVEFLHERIEGIQATARDCLLPGELIIR
                     ASTLR"
     gene            complement(5243..>6115)
                     /locus_tag="VHV99_22200"
     CDS             complement(5243..>6115)
                     /locus_tag="VHV99_22200"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEX3638139.1"
                     /translation="RDTFVAALKDADGGIGSSVKITPAMLEGASRLKALSTISVGFDQ
                     FDVTDLTRRGIVLAHTPDVLTESTADTVFSLILAAARRVVELAGWVKAGQWKHGVGPQ
                     QFGVEVQGKTLGIVGLGRIGGAVARRAALGFNMKVLYTNRSANPQAEQAYGARRVELA
                     ELLASADFVCLQVPLTPGTTHMIGAAELKSMKKSAILINASRGATVDEKALIEALQNG
                     TIHGAGLDVFDTEPLPADSPLLKMANVVALPHIGSATHETRHAMARNAAENLVAALEG
                     TLATNIVNRDVLKK"
BASE COUNT         1007 a         1999 c         1918 g         1191 t
ORIGIN      
        1 tcccgacccc gtaccaccgc cgtgagctga cgtggtctgc gtagcagcca ccggctggct
       61 ccgcggtgac tggctaccgg cgctaccgtc ctgcttcgcg cctgcggctg gcggcggggg
      121 cgtctgggtt cccgtatcct ccgtcggtga ctgtgtttgt acggacttgt ccgaactgtc
      181 gggcttggcc tgtgtggcag attctccagt actactttgc gattgcgtcc cgacgttgtg
      241 cgcatccggg tgtgtttgcg tgctcttcga attgtccgac ccggtttgag tctcagcgga
      301 catgcttttg acttgcgtct gtgattgcac ggacacgggt accgatgtgt gctgtgaggc
      361 agaggtatgg gtagatggcc tcgtggactg cgtggcggca tcctgacttg ttgggcggtg
      421 ctgcgttgct tgtgtcgcac ccgcatcggt ttgcgtaccg cttgcaacct gaaatgggcc
      481 ggcttgggtg gccgttgtcg tcttcgtacc ttgcgcccta tccgttgacg cgtgggtctg
      541 gtcagtctgc tccgacgcat ctgcctttat ctgaacgggc tgagggggag gcgtgccggc
      601 cgatgtgggc tgaactgcgg tctgtgttgg cacagagcgt gtcgcgggcc catgctgact
      661 gccacgctcc tgcggggcag ctttgacctg aactcccgca ttgttttggc caggcgcttg
      721 cgtcgttgtc ccttgactaa tctgcgaacc ttgcgcggtt tgcgtcgcct ggtggctgtt
      781 cggcgaacca gccggcctgc gcgggtcatt cgacgccgga tcggtcgagt gctctcctgc
      841 acttcgtggg cgatcatggt ctggtccctg cgagtttgga gtctgcgtac cggaatgagt
      901 gggcgatgtg gtctgcaccg aggcttcgtg ggtttggggc gtggactgcg ccccctgcga
      961 gcgcgtcggc gtatttgcct gtgtttgctt gcctgactgg ttgggctgta cctgcgtacc
     1021 cgtgtcggac ttgctgatcg gcgcgggctg tgtaccagcg ttggcggttt gcggctgaac
     1081 ggtctgcgtc ccacgatctg tcggcgcgtt acgcatgctc ttcaactcga tctgccctcc
     1141 ggaggcggtc tggccggaat gctggggtac ttcgtagata ccttgccgtc gtggtgaggt
     1201 ccgataactc gacctcatat ccttcttgtg gggaaccctt gccagatcaa tgcctgtggc
     1261 aatgccattg gcgatgtcct tgctgagctg caaggcgaac tgtttagtct cctgtttatc
     1321 cttcgcgtgc gcgaggcttc ctgcatcctt gatcgctttt gtgccgcttc cgatcagatt
     1381 accgccaagg tgacctgcgc gattcgagcg ttgtatcagg ccgggccgtg acagcgaacc
     1441 cgttggcgtg gagccgccgc ccggcatgga tgggggcacc gcgctgatca ggcctccaac
     1501 cttgaactgg agtgaattgc cacttggcgg tcccgcagtt ggactaggcg atgcctgtgg
     1561 gcctgtattc gtagggcttg acccaggacc ggccggcgca cctcccgcac cgttatcttt
     1621 gtccggcttt ttttcctgct caccctcctt tgctcgaccc gacaccgagt ggccggcatc
     1681 tcgcccctca ccggctgcag gctccttttt gcggtcgata agcccgccct ccctgcccgg
     1741 ttccgcgccg gaccgcactg atggagttgt cgggtagatg cgaggctggc ggctgggcgc
     1801 agccggcgtt gttccacccg caccttgatt ctcgtcaacc tgatacatcg catgaccctt
     1861 cgcgtcgact ttcatagtcc acctccctgg tgtcacaatg tctgaatccg cagaacgatt
     1921 gtggcgacgc aagcgatcct tccggtatca catatgtagt gtccgcattg agagaacctc
     1981 cgtatttctg ccatcggtcc ggcctggcga aaccggtcac gatgaatcac aacgcgatgt
     2041 cgccgatgca aaaaatatat ccagatgcat ctggcatgat gcgaacatcc agccgcagcg
     2101 cggcgccacc ctcctcactc cagatcgctt catgacagaa ctcaaaaacc tcgacctgcg
     2161 caccgatgca gccgcccgcc gcgtcgtcaa aaatgaaacg gtgactgtcg agttcgccgc
     2221 taccgaaggc gaactgatga gcctggaagg tccaaaccgt tacgcccgtg gcgatgcgct
     2281 gatcactgga tcgaccggcg accgctgggt tgtttcgcgc gaacgcttcg acgccaaata
     2341 cctgccggaa gacagcaccc gcgcccacgg cgaaccgggt gcgtaccgca accgtccggc
     2401 tgtggtgctg gccaaacaga tgaatgaagc gttttcgctg gctcgctcag cgagcggcgg
     2461 cgacgtactg cacggcgcgg ctggcgactg gatcatgcag tacgcgcccg gcgactacgg
     2521 cgtggtgcag gccgcacgtt ttgcgaaggt ctataagctg gcggattgag tcacggcggg
     2581 tggtcacagc tgttgagttg cggctgtcga gttgcggctg tcgagttgcg gctgtcgagt
     2641 tgcagccctg cgatcacgtt tgtccgactg cgcccggacc cgccttcaaa ccgggagcct
     2701 tgagtcgacc gcttcaagca aactcgtcgc cgagatccac tgtcacttcg cgcggcaccg
     2761 tctcgccgtt cgcatactgc tcttcgagcg agccgatgct tgcttcgaca tacgcccgca
     2821 gcaccgcgaa actccggcca cgcagccgcg ccggcatcgc gcgataacgc gccaacgccg
     2881 ccggcgtgat caccacggcc atcacgatcc ggcgcgcggc cgcaccgaaa cgcattgcaa
     2941 cccagtgaat cgccaacgtc ggcgtgccgt cttcgcccgc acgctggatg aactgcgtct
     3001 gctcgggaaa cagatcgctg atgacgcgcg ccaattcctc gaactcggga ttggcgcagt
     3061 cgtattggta tgcttccatg aggtatgccg aaaggatgag cctgaacggt gtaccgaaag
     3121 ggtaccccga aagttagaag tcgcgaatgg cttgcgcgac cagcgcctta ggcggcgacc
     3181 gccggccggc ggaacacgct gaacaacacg cccgcgacga tcaccgcgcc caccgcatac
     3241 gcaccgtcca ccgctacgag cgggacgagc tgcgcctgga ataatgcagc cggcacgtcc
     3301 accagcgcgt gcagcacgat cgcgagcggc aggatgccgc gccagcccgc gcgcacgccg
     3361 cgccacatca gcaccgacaa gccgatctgg aacaccagcg cggccacccg ttccagcgcg
     3421 aaaatcccag ccgtctgcgg cgtcaggctg gcgaggatca ggtggatgcg catcaccgaa
     3481 tcggtcggca gattgctcag gtaaccgtcg agctcgccac ggttttcgaa aacggcgaac
     3541 aggatccatt gaatctgcac cagcacgccg acgagccacg cttcagcgcc gccgtgcccg
     3601 aggccataag tgagcgccgt gccgtcgccc ttgcccgcca cgcccgatgc cgacttcgcc
     3661 gccgcccgct tgagcagcag ccgcatgccg atgaaacgcc ccacttcctc acagacaccc
     3721 gccaccagcg cgccgtacac cacgaaggcc agcggattgg acaggaaagt agcggtcgct
     3781 tcattccgat gcagcaggta gtcgttcagc gcgcgctcga tcaccatcgc gaacaacgcg
     3841 aagatggcga tgccggtgat cgcgtcgcgc gggttcagtg cgagcgtgcg gcgcaggcgg
     3901 cggtagatca ggaagggtaa ggcggcgacg aacagggtcg cgacggcgag gctcgagagg
     3961 gtaagcggtg caacaaccat gagtttcctt gcgtttgacc ggcgcgcacc cgctgcgtgc
     4021 cgcagcccga atgatcgcag atcagcgcga agtcagcgca aagttgaggc gcggatgatc
     4081 aattcgcctg gcaggaggca atcacgcgcg gtggcttgaa tcccttcgat gcgctcgtgg
     4141 agaaactcga ccgcgcggta gccgatctcg taggtcggct ggcggatagt ggtgacgccg
     4201 gtcaattcgg cccattcagg atcgtcgatc gacagcagcg cgacgtgcgt ctgccaatcc
     4261 gcgccgtagc gcgctttcag atgcagcgcg aggcacagcg cgaccggcgc gttggccgcg
     4321 aacaaggcga tgcggctggg tttgctgggg ggagtggttg tggcgttgcc tgcggcatcg
     4381 ccggtgctga tgcgtgctgc gttatctgcc ccgctggcca gggcgccacg aggaccgccg
     4441 gctgcatcga gggcgcagtc cagttcggcg agggcgcgct cgacggctgc cggctccgca
     4501 aggttcagca ccagcgtatg cccacgcgcg ccgcccttgc cgcccttgct gccttcgctt
     4561 ttcccactag tcctgccggc atggcccccg ccaccctcac ccctcatcac ctcgcgaaac
     4621 gccgcctcgc gcagttgccg cgaactgacc tgctcgaagg gctgcaccac aaaccagata
     4681 tcgtcaaagc cgctttcgag cagatgccgc gtgccgagtt cagctgccgc ccggttgtcc
     4741 agtccgacca tgtcggcgac gagcccctcc accgaacggt cgaccagcac cgccggaatg
     4801 ccgccgcccc ctaccggccg cagcgtttcc tcacgcacgc cgagcgcgtt gacgatcacg
     4861 ccttccacac gatacgtggt cagcagttgc agatagcgcc gctccatttc gacttcattg
     4921 gccgcgtggc agatcagcgg catcagaccg agcgcgtgac aagcggcttc cacgccttgc
     4981 agcacttcca cggtataggg gttggtcaag tcggcgagca gcatgccgat caggcgattg
     5041 cggccacgct tgaggccgcg cgccatctgg ttcggctgat agtcgagccg ctcgatggcc
     5101 gcctcgatgc gtgcccgcaa ctcaggcgac agcacgctca tctcgccatt cagatagcgc
     5161 gagatgctgg tcttgccggt gccggcttcg cgcgcgacat cggtaatcgt cgcgcggcgc
     5221 ggggcggcaa gcggcgtcgt gctcatttct tcaacacgtc gcggttgacg atgttggtcg
     5281 cgagcgtgcc ctcgagggca gcaacgaggt tctccgccgc attgcgcgcc atcgcatggc
     5341 gggtctcgtg cgttgccgaa ccgatatgcg gcagcgcgac cacgttcgcc attttcagca
     5401 gtggcgaatc ggccggcagc ggttcggtgt cgaacacgtc cagacccgcg ccgtgaatcg
     5461 tgccgttttg cagcgcttcg atcagcgcct tttcatcgac cgtcgcaccg cgcgatgcgt
     5521 tgatcagaat cgcgctcttc ttcattgact tgagttcggc cgcgccgatc atgtgcgtcg
     5581 tcccgggggt gagcggcact tgcaggcaga cgaagtcggc gctggcgagc agctccgcca
     5641 actcgacgcg gcgcgcgccg taagcctgtt ccgcctgcgg gttcgcgctg cggttcgtgt
     5701 acagcacctt catgttgaag ccgagcgccg cgcgccgcgc gaccgcgcca ccaatccgcc
     5761 ccaagccgac gatgccgagt gtcttgccct gaacctcgac gccgaactgc tgaggcccga
     5821 caccgtgctt ccactgtccc gccttcaccc acccggcgag ctccacaacc cgccgcgccg
     5881 cggccagaat cagtgagaac accgtatccg cggtggactc ggtgagcacg tccggggtgt
     5941 gcgcgagcac gatgccgcga cgcgtgagat cggtaacgtc gaactgatcg aagcccaccg
     6001 agatggtcga cagcgccttc agccggctcg cgccttcgag catcgccggc gtgattttca
     6061 cgctcgaacc gatgccgccg tccgcatcct tcaaggcggc gacgaacgtg tcacg
//
LOCUS       DATASH010000830        18836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6865306,
            whole genome shotgun sequence.
ACCESSION   DATASH010000830 DATASH010000000
VERSION     DATASH010000830.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 18836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18836
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6865306"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="VHV99_22205"
     CDS             <1..512
                     /locus_tag="VHV99_22205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="allophanate hydrolase"
                     /protein_id="HEX3638140.1"
                     /translation="TNATDATDAPPAANSPAATAAKTAPTIDPLPFQEPSITLAVVGA
                     HLRGQPLNWQLQEAGARFIDATHTSSAYSLYALANTQPPKPGLVRVTNQQGVPIAVEV
                     WDVPLRSFGKFVADVPAPLGIGTVQLASGLTVKGFICEPSAVSDGSGATDITPFGGWL
                     AYLDSLNVN"
     gene            609..1706
                     /locus_tag="VHV99_22210"
     CDS             609..1706
                     /locus_tag="VHV99_22210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX3638141.1"
                     /translation="MTSRRNFIKSASLLTLGNILPIQSVFAAPKTTVGVIYVGARGDY
                     GYNQAQATAAAVIKKMPDVKVVEEENVPETIAVQKTMEAMIEQDGATLIFATSFGYFD
                     PHVLKMAAKYPKVRFAHCGGLWKQGNPSNITSYFGYIDECQYLNGVVAGHMTKSKKLG
                     FVAAKPIPQVLRNINSFTLGAQSVDPSITTQVIFTGDWSMPVKEAEATNSLVDQGCDV
                     LTCHVDGPKVVVETAEKRGAMSCGYHASQAALAPKGYLTGAEWDWATPYRQLVTGAQA
                     GTPQPNVLRGGLKEGFVKMSPYGAKVTAQAKQNADSIKSNMVAGNFVIFKGPMKDNKG
                     GTAIASGASHIQTDYTLESMNYLVAGVVGQI"
     gene            1763..2878
                     /locus_tag="VHV99_22215"
     CDS             1763..2878
                     /locus_tag="VHV99_22215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3638142.1"
                     /translation="MRPNAPTARVMLSLLPALPTALAVLGTLLLFSLFLLVQGQPVLD
                     AIGLIFEGAFGSSFAWQSTLLRASPLMLTALCVALPAQVGLIVIGGEGALALGGMCAA
                     IVPQMLPPGTPWLIATPLMAVAGMLAGGFWIGAVGALRQWRGVNETISSLLMSYIAIA
                     LFNHLVEGPLRDPASLNKPSTYPVPDAMMIGSIPGLDTHWGLFWGVVACIAAWVFVKF
                     STKGFAMRVVGGSHRAARLVGLPVNLLALTACVLGGAAAGLGGMFEVAAVQGSANASL
                     LAGYGYAGILVAFAARQNPLAIIACALMIGGIEASGSLLQRRLDLPDATTLVLQGLLF
                     ANLLAWEALGGRIAAWRVKLQAAAQTDLAVQLEQTHA"
     gene            2871..3797
                     /locus_tag="VHV99_22220"
     CDS             2871..3797
                     /locus_tag="VHV99_22220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3638143.1"
                     /translation="MPDMHSPIVILLLSLFAGAIRVSTPYLFVSLGECLTEKGGRVNL
                     GLEGILVSGAMSGYAGAFLTGSPWAGVLAAGLVGLLLGCLHGLVCSLPRVSDIAFGIA
                     LMLLGTGLAFYLGKPFIEPQAPMLPFIDLGTWTSSPQLHNALHINLLFVIGVVLAFVL
                     QWGLRNTRWGMVLRLVGDHAETARAMGYPLTRVRIAATAVGGVFAGIGGAYLSLVYPG
                     SWNEGLSSGQGLMAVALVIFARWQPLRCLWAALLFGAAGALGPALQAVGVTSGYYLYN
                     AAPYVLTLAIMIVNCRPDRTLAGAPGELSLTR"
     gene            3966..4700
                     /locus_tag="VHV99_22225"
     CDS             3966..4700
                     /locus_tag="VHV99_22225"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013698609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEX3638144.1"
                     /translation="MNRFIEAKPYPWPYDGNLRPDNTALVIIDMQTDFCGHGGYVDKM
                     GYDLSLTRAPIEPINSVLKPMRELGFTIIHTREGHRPDLSDLPANKRWRSRRAGTDGV
                     GIGDDGPCGKILVRGEPGWEIIDELKPLPGEIIIDKPGKGSFCATDLELILRTRRIVN
                     LVLTGITTDVCVHTTMREANDRGFECTVLADCCGATDKGNHDAALHMITMQGGVFGTV
                     SDSHALLATLLATTAAPAAALATSGR"
     gene            4704..6371
                     /locus_tag="VHV99_22230"
     CDS             4704..6371
                     /locus_tag="VHV99_22230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3638145.1"
                     /translation="MSIAPKALAVEVLNASKSFGAFRALDSVSIKVKAGTIHALLGEN
                     GAGKSTLVKGLVGYGVLDDGQIAADGREVHIASPRDAQALGIGMVYQHFTLASGLSVE
                     ENLLLARGQMPWKIDWKGERAALAAFMQRMPFRLPLDTPVAMLAAGEKQKLEILKQLY
                     LQQRFLILDEPTSVLTPQEADEVLGLMRDLTSRGELTVLMITHKFREVMAYADDVTVL
                     RKGRHVGTSAVADTSRDELATWMMGTAVTRASKDVPLGGAPKEVPLENEAPVMAAVTA
                     ERMPRKPVDDTAQVRLALRDVSVDDDRRHTAVKHATLSVRAGEILGLAGVSGNGQKEL
                     VEALVGQRQVKAGDMQVAGKAYHATREEMTQRRVFAIPEEPLKNACVAAMSVAQNLAL
                     RDFDRAPLRRGGWWLDRRAMRERAAQLISEFNVRPPLPERAIGTLSGGNVQRAVLARE
                     LGQPVDVLIVANPVFGLDFASVADIHARLMAARDAGAAVLLVSEDLDELLELADRIVV
                     IAEGKLVFETPAARADRTVLGQHMAGHGSDTPAHHAAAEETMKEAIG"
     gene            6368..7057
                     /locus_tag="VHV99_22235"
     CDS             6368..7057
                     /locus_tag="VHV99_22235"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEX3638146.1"
                     /translation="MTQTTALTIDAQPGPFTLAPTKTALIVIDMQRDFIEPGGFGESL
                     GNDVSLLAEIVPTVAALLAFARRHHWLVVHTRESHAADLSDCPAAKRLRGAPNARIGD
                     AGPMGRILIRGEPGNAIVEPLAPLAGELVIDKPGKGAFYATRLGEELAMRGVTHLVFA
                     GVTTEVCVQTTMREANDRGYDSLLIEDATASYFPAFKQATLDMVRSQGGIVGWTAPFS
                     SLTKFDETIPA"
     gene            7054..7455
                     /gene="hpxZ"
                     /locus_tag="VHV99_22240"
     CDS             7054..7455
                     /gene="hpxZ"
                     /locus_tag="VHV99_22240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalurate catabolism protein HpxZ"
                     /protein_id="HEX3638147.1"
                     /translation="MKVNQPDVVAQVQAAFIEYERALVDNDVAAMNALFWHTPETVRY
                     GIAEIQHGGEAIRAWRETCEPVPKSRKLHRTVVTTFGTDFATVSTEFTSDATPLIGRQ
                     MQTWARLGPTDGWKIVAAHVSLIAMPWNADK"
     gene            7477..8253
                     /locus_tag="VHV99_22245"
     CDS             7477..8253
                     /locus_tag="VHV99_22245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX3638148.1"
                     /translation="MNDDPTNLLETPAESDTPGSAGTQGMPAAPAAANPLAEQVYQQL
                     KSDIFSFRLFPGDRFSENGIAQHYGVSRTPMRDGLFRLQREGYLEVGFRRGWKVAPIN
                     FDQLDQLYDLRIVLEVAAVERVGAGGELADAAVDALKAIWCVEPDLRESDPVNLFGMD
                     ENFHRQLVAATGNAEMLRVHNEVTERIRIVRRLDFLKPHRTSATYDEHATMLNLIERN
                     RLTEAIILLRAHITQSKLEVRKITLSMLAAARDSKLPFVS"
     gene            complement(8472..9590)
                     /locus_tag="VHV99_22250"
     CDS             complement(8472..9590)
                     /locus_tag="VHV99_22250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HEX3638149.1"
                     /translation="MSKLHSAVKVGPYQFAHRVVLAPLTRMRAEPGARPGPLMAEYYA
                     QRTSEGGFLIGEATIAAPNGNGYLGAPGLYDDSQIAGWKQVTDAVHAKRGRIFLQLYH
                     AGRQSNSQLQPGGVQPVAPSAVPHGGVAYTEAGWIPNTPSRALTIPEIAELVESFRTA
                     AIRGKEAGFDGVELHAANGYLFDQFLQDGSNKRTDIYGGSFENRARFLIEATRAVISV
                     WGSERVAVRLGPSGTWGDMSDSNPEGLFSYVAQELAKLNLAYLHLIEPRVLGNVDDDS
                     KDPNPVAAQRIRKHYKGVIIAAGGFKAATAEAVLQAGDADLVAFGRDFIANPDLPERL
                     RLRLPLNPYDRPTFFGGTDVGYTDYPFYSTETTDAVAA"
     gene            9826..10710
                     /locus_tag="VHV99_22255"
     CDS             9826..10710
                     /locus_tag="VHV99_22255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013094288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX3638150.1"
                     /translation="MDSLSGISVFVQAAETRSFTEAGRLLGVSSSAVGKSVARTEERL
                     GVRLFHRSTRSITLTAEGALFLERCRRILGELEAAEAELSDAAGSPRGRLRISTPQLS
                     ELIMPALNGFMQQYPDIELDVDLSDRMVDIIEEGFDAVIRTGEQHDSRLVSRRLGSCA
                     QVLVASPGYLEKYGVPKHPSELVQHACLLHKFPASGKLERWPFQLAASEAEPELPQTF
                     VANTIEVLGFLALQNKGLTFVPTFLVRDALASGALQIVLDNFIDQTVTFWILWPASRY
                     ASPKLRVFIDYVSQNLRI"
     gene            10993..11172
                     /locus_tag="VHV99_22260"
     CDS             10993..11172
                     /locus_tag="VHV99_22260"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3638151.1"
                     /translation="MLERLTGTAERKAAFLANVPLKRAGTPEEIADAIVFVASDKASF
                     ITGELIRINGGKTAS"
     gene            complement(11194..12237)
                     /locus_tag="VHV99_22265"
     CDS             complement(11194..12237)
                     /locus_tag="VHV99_22265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX3638152.1"
                     /translation="MFGFLRGYFSNDLAIDLGTSNTLIYMRGKGIVLDEPSVVSIRQE
                     GGPNGKKIILAVGKEAKQMLGKVPGNIEAIRPMKDGVIADFNITQQMIKRFIQMAHES
                     RMFAPSPRIIICVPCGSTQVERRAIKEAAHSAGASQVYLIEEPMAAATGAGLPVSEAT
                     GSMVVDIGGGTTEVGVISLGGIVYKGSVRVGGDKFDDAIVNYIRRNYGMLIGEQTAEA
                     IKKEIGSAFPGSEVKEMEVKGRNMSEGIPRSFTVSSNEVLEALTDPLNQIVSAVKIAL
                     EQTPPELGADIAERGIMLAGGGALLRDLDRLLAEETGLPVFVAEAPLTCVVRGSGMAL
                     ERMDKFGGAFSYE"
     gene            12558..13772
                     /locus_tag="VHV99_22270"
     CDS             12558..13772
                     /locus_tag="VHV99_22270"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3638153.1"
                     /translation="MDETTQTTRCYDIISDEAEFRALQPEWDTLWSKANGYYYQSFSF
                     CWLAWKHVAKPQGRKLKCIVCREDGQLVMIWPLETSKRALWTYLVPLGPQGGDYTSVL
                     VENGAAAPALIEGAWETARRRCGADFIHLPYVRDTLHLHTLVMQERRVLFTEAHNASA
                     ATLRGQGSWEEYGRTLGTLFGKRPGSFVNRLAKEGKVAVRVVDPSEEAETASIIAWMF
                     NCKRQWSERVGKHSVWIDLPEFECFLGKLIYSPDVPSMARLIVVTLDGAPVAGIIVSF
                     GNPWASAIFAGYDPQYGRCCPGLIAVEHCVKWAFDNGFDLDFGVGTERFKSYWARGEA
                     STAWTVQIINSVWGLAAIRARRLSRELVSRVKRIRQAGAAAPEAGIEDLPLTPAMTLQ
                     SSEAFGQRPEHN"
     gene            complement(13937..14107)
                     /locus_tag="VHV99_22275"
     CDS             complement(13937..14107)
                     /locus_tag="VHV99_22275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638154.1"
                     /translation="MTLLIYLAGWIILIGGVAWGLMALHVAQHTIAIVAVIMLGLAVI
                     TGATRARNRDRS"
     gene            complement(14346..14762)
                     /locus_tag="VHV99_22280"
     CDS             complement(14346..14762)
                     /locus_tag="VHV99_22280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEX3638155.1"
                     /translation="MTQLEKVLYTGKTHTTGGREGASRSSDGRLDVKLSTPGTPGSGT
                     NPEQMFAAGWSACFIGAMGLAAAKLKIALPADTAVDAEVDLGTTDGAYFLQARLNVSL
                     PGLDREAAEALANAAHQTCPYSKATRGNIDVVIKVI"
     gene            complement(14787..15074)
                     /locus_tag="VHV99_22285"
     CDS             complement(14787..15074)
                     /locus_tag="VHV99_22285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013338980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638156.1"
                     /translation="MKKHLIVAAIFAVGTAIATPVFASSGYGPAPHYNPIAGAPASQR
                     GQSALTVRAEYADANANANAAMQSFGGIRETVSQSGGPVTAVSARSFYVHH"
     gene            complement(15192..15437)
                     /locus_tag="VHV99_22290"
     CDS             complement(15192..15437)
                     /locus_tag="VHV99_22290"
                     /inference="COORDINATES: protein motif:HMM:NF025045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4148 domain-containing protein"
                     /protein_id="HEX3638157.1"
                     /translation="MKTSILALALAASVTTGLAHASETAATNAADPGVVQSQQWSAAG
                     SGKGVVLKTRADVRHELERARHDGQLVYLNSNLYRGS"
     gene            15720..16207
                     /locus_tag="VHV99_22295"
                     /pseudo
     CDS             15720..16207
                     /locus_tag="VHV99_22295"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            complement(16258..16572)
                     /locus_tag="VHV99_22300"
     CDS             complement(16258..16572)
                     /locus_tag="VHV99_22300"
                     /inference="COORDINATES: protein motif:HMM:NF023239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3331 domain-containing protein"
                     /protein_id="HEX3638158.1"
                     /translation="MYSRLQSRPDPARQKEPTFTVSVLEYLPNKLVVISWHDPTMCNY
                     EEQIWSPALARHSGRCALSGSRISRGDTVYKPRTRGRIEPLNSDAMILASTLKSVPGS
                     YA"
     gene            complement(16897..17025)
                     /locus_tag="VHV99_22305"
     CDS             complement(16897..17025)
                     /locus_tag="VHV99_22305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638159.1"
                     /translation="MSHALQTLLFIGVNAYVEFTFVAWLNRGVYRRTVDYAAVCIQ"
     gene            17399..17812
                     /locus_tag="VHV99_22310"
     CDS             17399..17812
                     /locus_tag="VHV99_22310"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3638160.1"
                     /translation="MPRRYSGSEPRTTILQKTPVVSIVDDDQSVRRALGSLVRSMGWE
                     ARMFESAEAFLASGDVSDTACLLSDIRMSGMSGVEMHERLVAQGQAPATIFISAYPTP
                     SLQEKVTGNGALVLLQKPCQVSVMTHWLSVALGAQ"
     gene            18189..>18836
                     /locus_tag="VHV99_22315"
     CDS             18189..>18836
                     /locus_tag="VHV99_22315"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012366933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3638161.1"
                     /translation="MEQQSESGRGRVAVVTGGAMGIGADVAERLARSGYHAVLSDINR
                     GAADETAGKLRAQGLSASAVVMDVGNPESIAEGFAAIARDAGRCDVLVNCAGIATMLP
                     FIDYPLDVYLKTMQVNVTGTLLCAQHAARMMRPNGCGRIVNIASVAGMRAVGTGRTAY
                     GTSKGAVIALTRQMAVELCDYGITANAVSPGPVDTPMTKALHSAQFREEYSRAIPL"
BASE COUNT         3506 a         5709 c         6135 g         3486 t
ORIGIN      
        1 ccaccaatgc aacggatgcc accgacgcgc cgcccgccgc gaatagcccg gccgcaaccg
       61 cagcgaaaac cgcacccacg atcgacccgt taccgttcca ggagccgagc atcacgctcg
      121 ccgtggtcgg cgcgcatctg cgtggccagc cgctgaactg gcaacttcag gaagccggcg
      181 cgcgttttat cgacgcgacc cacaccagct ccgcttactc gctttacgcg cttgccaaca
      241 cgcagccgcc gaaaccgggg ctggtgcgcg tcaccaatca gcaaggcgtg cccattgccg
      301 tggaagtctg ggacgtgccg ctgcgcagct tcggcaaatt cgtcgccgac gtgcccgcac
      361 cgctcggcat cggtaccgtg caactcgcca gcggccttac cgtgaaaggc ttcatctgcg
      421 agccgtcggc ggtgagcgac ggcagcggcg caacagacat caccccgttt ggcggctggc
      481 tcgcttatct cgacagcctg aacgtcaact gaacgtcaac tgaacgccaa ctgaacgtca
      541 actgaacgtc aactgaacgt caactgaacg ccagcgctat tcaaccccca accaggaatt
      601 tggacaccat gaccagtcgc cgcaatttca tcaagagcgc cagcctgttg acgctcggca
      661 atattctgcc gattcaatcg gtgtttgccg cgccgaagac gacggtcggc gtgatctacg
      721 tcggcgcgcg cggcgactac ggttacaacc aggctcaggc aaccgctgcc gccgtcatca
      781 agaagatgcc tgatgtgaag gtggtggaag aggagaacgt ccccgaaacg atcgccgtgc
      841 agaaaaccat ggaagcgatg atcgaacagg acggcgccac gctgattttc gcgacctcgt
      901 tcggttactt cgatccgcat gtgctgaaga tggcggccaa atatccgaag gtgcgtttcg
      961 cgcattgcgg tggtttatgg aagcagggca acccgtcgaa catcaccagc tacttcggct
     1021 atatcgacga atgccagtac ctgaacggcg tggtcgcggg ccatatgacg aagagcaaaa
     1081 agctcggctt cgtcgccgcc aaaccgattc cgcaggtgct gcgcaatatc aattcgttca
     1141 cgctcggcgc gcagtccgtt gatccgtcga tcaccaccca ggtgattttc accggcgact
     1201 ggtcgatgcc ggtgaaggaa gcggaagcca ccaacagtct ggtcgatcag ggttgcgatg
     1261 tgctgacctg tcacgtcgac ggtccgaagg tggttgtcga aacggcggaa aagcgcggcg
     1321 cgatgagttg cggttaccac gcgagccagg cggcgctcgc gccgaaaggt tatctgaccg
     1381 gcgcggaatg ggactgggcg acgccgtaca ggcaactggt cacgggggca caggccggca
     1441 cgccgcaacc gaacgtgctg cgcggcgggt tgaaagaagg tttcgtcaag atgtcgccgt
     1501 acggcgcgaa agtcaccgcg caggcgaagc agaacgccga ctcgatcaaa agcaacatgg
     1561 tggccggtaa tttcgtgatc ttcaaaggac cgatgaagga caacaagggc ggcacggcga
     1621 tcgcctccgg cgcgagccat attcaaaccg actacacgct cgaaagcatg aactatctgg
     1681 tggcgggcgt cgtcggccag atctgatggt ggcctgcctg ttgtgtaagt gtgcgtgagt
     1741 ttgcatgagc gaaggagccg tgatgcgtcc caatgcccct accgcgagag tgatgctgtc
     1801 gctcttgccc gcactgccga ccgcgttggc ggtgctcggc acgttgctgc tgttctcgct
     1861 gttcctgctg gtgcagggac agccggtgct cgatgcgatc gggctgatct ttgaaggcgc
     1921 gttcggctcg tcattcgctt ggcagagcac cttgctgcgc gcgtcgccgc tgatgttgac
     1981 cgcgctgtgc gtcgcgctgc ccgcccaggt cgggctgatc gtgatcggcg gcgagggcgc
     2041 gctcgcgctg ggcggcatgt gcgcggcgat cgtgccgcag atgctgccgc ccggcacgcc
     2101 ctggctgatc gccacgccgt tgatggcggt ggccggcatg ctggccggcg ggttctggat
     2161 cggcgcggtg ggcgcgttgc ggcaatggcg cggcgtcaac gagacgatca gcagcctgtt
     2221 gatgtcgtat atcgcgatcg cgctcttcaa ccatctggtg gaagggccgc tgcgcgaccc
     2281 cgccagcctg aacaagccgt ccacctatcc ggtgcccgac gcgatgatga tcggctcgat
     2341 ccccgggctc gacacgcact gggggctgtt ctggggcgtc gtcgcttgca tcgcggcgtg
     2401 ggtgttcgtc aagttcagca ccaagggctt cgcgatgcgc gtggtgggcg gcagtcatcg
     2461 cgcggcgcgt ctggtcggtt tgccggtcaa tctgctggcc ctgacggcgt gcgtattggg
     2521 cggcgccgcg gcgggactcg gcggcatgtt cgaagtggcg gcggtgcaag gcagcgcgaa
     2581 tgcatccttg ctcgcgggtt atggctatgc gggcattctc gttgcgttcg ccgcacgcca
     2641 gaatccgctc gcgatcatcg cgtgcgcgct gatgatcggc ggcatcgagg cgagcggctc
     2701 gctgctgcag cggcggctcg atcttcccga tgccaccacg ctcgtcctgc aaggcctgct
     2761 gttcgcgaac ctgctcgcgt gggaagcgct cggcgggcgc atcgccgcat ggcgcgtgaa
     2821 gttgcaggcc gccgcgcaga ccgaccttgc cgtgcaactg gagcagaccc atgcctgata
     2881 tgcactcacc catcgtgatc ctgttgctgt cgctgttcgc cggtgcgatc cgcgtcagca
     2941 cgccttatct gttcgtcagt ctcggcgaat gcctgaccga gaagggcggc cgtgtgaatc
     3001 tcggcctcga aggcatcctc gtgtccggcg cgatgagcgg ctatgccggt gcttttctga
     3061 ctggctcacc gtgggccggt gtgctggcgg ccggtttggt cggcttgctg ctcggctgtc
     3121 tgcacggcct cgtgtgctcg ttgccgcgcg tatccgatat cgcgttcggt atcgcgttga
     3181 tgctgctcgg cacggggctc gcgttctatc tcggcaagcc tttcatcgaa ccgcaggcgc
     3241 cgatgctgcc gttcatcgac ctcggcacgt ggacctcgtc gccgcaattg cataacgcgc
     3301 tgcatatcaa cctgttgttc gtgatcggcg tggtgctggc gttcgtcctg caatggggct
     3361 tgcgcaatac ccgttggggg atggtgctga ggctggtggg cgatcacgcc gagacggcgc
     3421 gcgccatggg ttatccgctc acgcgcgtgc gcatcgcggc gacggcggtg ggcggtgtgt
     3481 tcgcgggcat cggcggggcg tatctgtcgc tggtttatcc aggtagctgg aatgaagggc
     3541 tctccagcgg acaaggactg atggcggtcg cgctggtgat tttcgcgcgc tggcagccgt
     3601 tgcgttgcct gtgggccgcg ttgctgttcg gtgcggccgg cgcgctcggg cctgccttgc
     3661 aggcggtcgg cgtgacgagc ggctattacc tctacaacgc ggcgccgtat gtgctgacgc
     3721 tcgcgatcat gatcgtcaat tgccgtccgg atcgcacgct ggcgggcgcg ccgggggaac
     3781 tgagcttgac ccgctgaacc gtgcctgctg aatctggccc actgaccttg aaccgctgac
     3841 cttgaaccgc tgactttgaa ccgctgacgt tggcccgctg aacctgactc gacgagcccg
     3901 cggttgccac actgattttg ccctgccgtg cgtgccttca aggacccaat aaggatctca
     3961 tcaccatgaa ccgattcatt gaagcgaaac cctatccgtg gccctacgac ggcaatctgc
     4021 gcccggacaa cacggcgctc gtgatcatcg acatgcagac ggatttctgc ggccatggcg
     4081 gctacgtcga caaaatgggc tatgacctgt cgctgacgcg cgcgccgatc gagccgatca
     4141 acagcgtgct gaagccgatg cgcgagttgg gcttcacgat cattcacacg cgtgaagggc
     4201 atcggccgga cctctccgac ctgcccgcga ataaacgctg gcgcagccgc cgcgcgggca
     4261 ccgacggcgt aggaatcggc gacgacggcc cctgcggcaa gattctcgtg cgaggcgaac
     4321 caggctggga aatcatcgac gaactgaagc cgctgccagg cgaaatcatc atcgacaaac
     4381 ccggcaaggg ctcgttctgc gcgacggacc tcgaactgat cctgcgtaca cgccgcatcg
     4441 tcaatctggt gctgaccggc atcaccaccg acgtctgcgt ccacacaacg atgcgcgaag
     4501 ccaacgatcg cggcttcgaa tgcacggtgc tggccgattg ctgcggcgcg accgacaagg
     4561 gcaatcacga cgccgcgctg catatgatca ccatgcaagg cggcgtgttc ggcacggtgt
     4621 cggactcgca cgccctgctg gctacgctac tggccacaac ggcggcgccg gcagcggcgc
     4681 tagccacttc aggcagataa accatgagca ttgcgccgaa ggcattggct gtggaagtgc
     4741 tgaacgcgag caagtcgttc ggcgcgtttc gcgcattgga tagcgtctcg atcaaggtca
     4801 aggccggcac cattcacgcg ctgcttggcg agaacggcgc gggcaagagc acgctcgtga
     4861 aggggctggt cggctatggc gtgctcgacg acggacagat cgccgccgat ggccgcgagg
     4921 tccacatcgc gtcgccgcgt gatgcgcagg cgctcggcat cggcatggtg tatcagcatt
     4981 tcacgctggc gtccggcctt tctgtcgaag agaacctgtt gctcgcgcgc ggacagatgc
     5041 cgtggaaaat cgactggaag ggcgagcgcg ccgcgcttgc cgcgttcatg cagcgcatgc
     5101 cgtttcgctt gccgctcgac acgccggtgg ctatgctcgc tgccggtgaa aagcagaaac
     5161 tcgaaattct gaagcagctc tatttgcagc agcgcttcct gattctcgac gaacccacct
     5221 cggtactcac gccacaagaa gcggatgaag tgctcggcct gatgcgtgat ctgacctcac
     5281 gcggcgagct gacggtgctg atgatcacgc acaagttccg cgaagtgatg gcctatgccg
     5341 acgacgtcac cgtgttgcgc aagggcaggc atgtcggcac gagcgcggtg gccgacacca
     5401 gccgcgacga actcgccacg tggatgatgg gcaccgccgt tacgcgcgcc tcgaaggacg
     5461 tccccttggg tggtgccccg aaggaagtcc ccctggagaa cgaagcgccg gtcatggccg
     5521 cggtgacggc ggagcgcatg ccgcgcaaac ccgtcgacga cacggcgcaa gtacgtctcg
     5581 cactgcgcga cgtatcagtg gacgacgatc gccgacacac ggcggtcaag cacgcgaccc
     5641 tgtccgtccg cgcgggcgaa atcctcggac tggcgggcgt gtccggcaat ggacagaagg
     5701 aactggtgga agcgctggtc ggtcaacgtc aggtgaaagc cggcgacatg caggtggcgg
     5761 gcaaggccta tcatgcaacg cgcgaagaga tgacccaacg gcgcgtgttc gcgattcccg
     5821 aagagccgct caagaacgcc tgcgtcgcgg cgatgagcgt ggcgcagaat ctcgcgctac
     5881 gcgacttcga tcgcgcaccg ttgcgacgtg gcggctggtg gctcgaccgg cgtgcaatgc
     5941 gtgagcgcgc cgcccagttg atcagcgagt tcaatgtgcg tccaccgttg cccgaacggg
     6001 cgatcggcac gctgtcgggc ggcaacgtgc agcgcgcggt gctggcgcgt gaactcggcc
     6061 agccggtgga tgtgctgatc gtcgcgaatc ctgtgttcgg tctggatttc gcgtcggtcg
     6121 ccgacatcca tgcccgtctg atggccgcac gtgatgcagg cgcggcggtg ctgctggtca
     6181 gcgaagacct cgacgaactg ctcgaactcg cggaccgcat cgtggtgatc gcggagggca
     6241 agctggtctt cgagacgccc gcggcgcgcg cggatcggac cgtgctgggt cagcatatgg
     6301 cagggcacgg cagcgatacg ccggcgcatc atgccgccgc cgaggaaacc atgaaggaag
     6361 ctatcggatg acacagacca ctgcgctgac gattgacgcc caaccaggcc cgttcaccct
     6421 ggcgcccacg aaaaccgcgc tgatcgtgat cgacatgcaa cgcgacttca tcgagccggg
     6481 cggcttcgga gaatcgcttg gcaatgacgt atcgctgctg gccgagatcg tgccgaccgt
     6541 cgcggcgttg ctggccttcg cgcgcaggca ccactggctc gtcgtgcata cgcgtgaatc
     6601 gcatgcggcg gatctgtccg actgcccggc ggcgaaacgg ctcagaggcg cacccaacgc
     6661 ccgcatcggc gatgcaggac caatggggcg catcctgatt cgcggcgagc ccggcaatgc
     6721 gatcgtcgag ccgctcgcgc cgcttgccgg cgaactcgtg atcgacaaac ccggcaaagg
     6781 cgcgttctac gccacccggc tcggcgaaga gctcgcgatg cgcggcgtca cgcatctggt
     6841 gttcgccggc gtgaccaccg aagtctgcgt gcagaccacg atgcgcgaag ccaacgaccg
     6901 cggttacgac agtctgctga tcgaagacgc gaccgccagc tatttcccgg cgttcaagca
     6961 ggcgacgctc gacatggtgc gctcgcaagg cggcattgtc ggctggaccg cgccattttc
     7021 ttcattgacg aaattcgacg agacgatccc ggcatgaaag tgaatcaacc cgacgtggtt
     7081 gcccaggtgc aggcggcatt tatcgaatac gagcgggcgc tggtcgacaa cgacgtcgcg
     7141 gcgatgaacg cgcttttctg gcacacgccg gagacggtcc gctacggtat cgccgaaatc
     7201 cagcatggcg gcgaggcgat ccgcgcatgg cgcgagacct gcgagccggt gccgaagtcg
     7261 cgcaaactgc atcgcaccgt ggtgacgacc ttcggcaccg acttcgccac cgtcagcacc
     7321 gaattcacca gcgacgcgac accgttgatc ggccgtcaga tgcagacgtg ggcgagactc
     7381 ggtcccaccg acggctggaa aatcgtcgcg gcgcacgtca gccttatcgc catgccatgg
     7441 aacgccgata aatagaaaaa aggggatgtg gcagcaatga acgacgaccc gacgaatctg
     7501 ctcgagactc ccgcagagtc tgacacgcca ggctctgcgg gaacgcaggg catgcctgcc
     7561 gcgccggctg cggccaatcc gcttgccgag caggtgtacc agcaactgaa gagcgatatc
     7621 ttcagcttcc gcctgtttcc cggcgaccgc ttttcggaaa acggcatcgc gcagcactac
     7681 ggcgtgtcgc gcacgccgat gcgcgacggc ctgtttcgtt tgcaacgcga ggggtatctg
     7741 gaggtcggat tccggcgcgg ctggaaagtc gcgccgatca atttcgacca gctcgatcag
     7801 ctctacgatt tgcgcatcgt gctggaagtg gcggccgtcg agcgcgtggg ggcgggcggc
     7861 gaactcgccg atgcggccgt cgacgcgctg aaggcgatat ggtgcgtcga gcccgactta
     7921 cgcgagagcg atccggtgaa tttgttcggc atggacgaga acttccaccg tcaactggtc
     7981 gccgcgaccg gcaatgcgga gatgctgcgc gtgcataacg aagtgactga gcgcatccgc
     8041 atcgtgcgcc ggctggactt cctgaaaccg caccgcacga gcgccaccta cgacgaacat
     8101 gccaccatgc tgaacctgat cgaacgcaac cggctgacgg aagcgatcat cctgctgcgc
     8161 gcgcatatca cccagagcaa gctggaagta cgcaagatta cgctgtccat gttggccgcc
     8221 gcgcgcgata gcaagttgcc gtttgtgtcc tgagtgagct cagtaccctg ggaattcgcg
     8281 aagggcgctg aaaaaatctc cgtcgcgaga agagtctggt catcgtcagg atggggacca
     8341 gcgccaatgt cgtgggcagc caatgagcgc tagccgacat gagcgagaac gcgaagatcg
     8401 acacgctcag gcacgggacg atcacgcggc tcggaccgag gcggtccact gactttccgc
     8461 cgatgaaccg cttacgctgc tacagcgtcc gtggtctcgg tgctgtagaa cgggtagtcg
     8521 gtatagccga cgtcggtgcc gccgaagaag gtcgggcggt cgtacgggtt cagcggcaac
     8581 cgaaggcgca ggcgctcggg cagatccggg ttggcgatga agtcgcggcc aaatgcgacc
     8641 aggtcggcgt cgcctgcctg cagcacggct tcggcggtag cggccttgaa gccgccggcg
     8701 gcgatgatga cgcccttgta gtgcttgcgg atccgttgcg cggcgaccgg gttcgggtcc
     8761 ttgctgtcgt cgtcgacgtt gcccagcacg cgtggctcga tcaggtgcag ataggcgagg
     8821 ttcagtttcg ccagttcctg cgcgacgtag ctgaagagtc cttccggatt gctatccgac
     8881 atatcgcccc aggtgccgct cgggccaagg cgcacggcca cgcgctcgct gccccacacg
     8941 gagatgacgg cgcgggtggc ttcgatcagg aagcgggcgc ggttttcgaa cgagccgccg
     9001 tagatgtcgg tgcgcttgtt gctgccgtcc tgcaggaatt ggtcgaacag gtagccattg
     9061 gcggcgtgca gttcgacgcc gtcgaagccg gcctctttgc cgcgaatcgc ggccgtgcgg
     9121 aagctctcca ccagctcggc gatctcgggg atggtcagcg cgcggctcgg cgtgttcgga
     9181 atccagcccg cttcggtgta ggcaacgccg ccatgcggca cggcggaggg cgccaccggc
     9241 tgcacgccgc ctggctgcaa ctggctgttg gattggcgtc cggcgtgata cagctgcagg
     9301 aagatgcggc cgcgtttggc gtgcacggcg tccgtcacct gcttccagcc cgcgatctgg
     9361 ctgtcgtcgt agaggccggg tgcaccgagg tagccgttgc cgttgggtgc ggcgatggtg
     9421 gcttcaccga tcaggaaccc gccttctgac gtgcgttggg catagtactc ggccatcaac
     9481 gggccgggcc gggcgcctgg ttcggcacgc atacgggtaa gcggtgccag cacgacgcga
     9541 tgggcaaatt ggtaaggacc gactttgact gcggaatgaa gtttggacat gacttcctcg
     9601 agatggggtg gctggtgacg cttgatcaac agcgggcttt cgaccttgag gagcagtgta
     9661 tggacggacg gggataagaa aaataggcta aggttccgaa tatatcggac aagcaagtcc
     9721 gtaatcgact cgttcgctgc ggcagaacga gtcgcagccg aggttcgaac gcttgcctgc
     9781 gtcgcccggc tggcacaaat cagcttcggg cctcgggatg cggcgatgga cagcctcagc
     9841 ggtatttcgg tgttcgtgca ggcggcggaa acccgcagct ttacggaggc gggacggctg
     9901 ctcggcgtat cgtcttcggc ggtcggcaaa agcgttgcgc gcaccgagga gcgcctcggc
     9961 gtgcggctct ttcaccgcag cacgcgcagc atcacgctga ccgccgaagg agcgttgttt
    10021 ctggaacggt gccggcgcat cctcggcgag ctcgaagcgg cggaagccga actgtccgac
    10081 gccgccggca gtccgcgcgg cagactgcgg atcagcacgc cgcaactgtc cgagctgatc
    10141 atgccggcgc tgaacggctt catgcagcaa tacccggaca tcgaactcga cgtcgatctg
    10201 tccgatcgca tggtggacat catcgaagaa ggcttcgatg cggtgatacg cacgggtgag
    10261 cagcacgact cgaggctggt gtcgcggcgc ctcggttcct gcgctcaggt gctggtcgct
    10321 tctcccggct atctggagaa gtacggcgtg cctaagcatc cgtccgaact cgtgcagcac
    10381 gcctgcctgc tacataaatt tcccgcgagc ggcaagctgg agcgctggcc gtttcaactc
    10441 gccgcgtcgg aggcggagcc ggagttgcca caaaccttcg tcgccaacac cattgaagtg
    10501 ctgggttttc tggcgcttca gaataagggg cttaccttcg tgccgacctt cctcgtgcgc
    10561 gatgcgctgg cgagcggtgc gttacagatc gtgctcgaca atttcatcga ccagaccgtc
    10621 accttctgga tcttgtggcc ggcaagccgt tacgcttcgc cgaagctgcg cgtgtttatc
    10681 gactacgtca gccagaatct gcggatctga cctgacaggg gcggtaaaga atcggctaag
    10741 agccgcgcca caggactaaa gtgtccgaaa tgatcggaac ctcagcctgt ttatcagcaa
    10801 ttgcctgaac tctactattg gcctgtcgct gcacagatgg tctgcagagc gtcaaacggg
    10861 agagttcttg tgaacagtat cgtcagtcga gctgcaggct ccggcaaaat cagggtcggc
    10921 gtcgtcgggc tcggcaacgg gtcggaatcg cacgggacag cgtgccccgc tttcgttcag
    10981 cacgagaccg cgatgcttga acggctaacc ggcaccgcgg agcgaaaagc cgcttttctc
    11041 gcgaacgttc cgctcaagcg cgcgggaacg cccgaagaga tcgccgacgc catcgtgttc
    11101 gtcgcatctg acaaggcgag ctttatcacg ggcgaactca tcaggatcaa cggcggaaaa
    11161 acggcctcgt aagccatcgt tcacgaactg ggcttactcg tacgaaaaag ccccgccgaa
    11221 tttgtccatc cgctccagcg ccattcccga accgcgcacc acacaggtca gcggcgcttc
    11281 ggcgacgaac accggcaggc cggtttcttc cgcgagcaga cggtccagat cgcgcagcaa
    11341 ggcgccgcca cccgcgagca tgatgccgcg ctcggcgatg tcggcgccca gttccggcgg
    11401 cgtctgctcc agcgcgatct tcaccgccga gacgatctga ttcagcggat ccgtgagcgc
    11461 ctcgagaacc tcgttgctgg acaccgtgaa gctgcgcgga atgccttcgg acatattgcg
    11521 gcccttgact tccatctcct tgacctcgga gcccggaaac gccgaaccga tttccttctt
    11581 gatcgcctcg gcggtctgct cgccgatcag catcccgtag ttgcggcgaa tgtagttgac
    11641 gatcgcatcg tcgaacttgt cgccgcccac gcgcaccgaa cccttataga caatgccgcc
    11701 gagcgagatc acccccacct cggtcgtgcc gccgccgata tcgaccacca tcgagccggt
    11761 cgcttccgac accggcaaac ccgcaccggt ggccgcggcc atcggctctt cgatcaggta
    11821 gacctgcgag gcgcccgcgc tatgcgcggc ttccttgatc gcgcggcgct cgacctgagt
    11881 cgagccgcac ggcacgcaaa tgatgatgcg cggcgacggc gcgaacatcc gcgactcatg
    11941 cgccatctgg atgaaacgct tgatcatctg ctgggtgatg ttgaagtcgg ctatcacgcc
    12001 gtctttcatc gggcggatcg cctcgatatt gcccggcacc ttgccgagca tctgtttcgc
    12061 ttccttgccg acggccagaa tgatcttctt gccattcggg ccgccttcct gacgaatcga
    12121 gaccactgag ggttcgtcca gcacaatgcc cttgccacgc atgtagatca gggtgttcga
    12181 cgtgccgagg tcgattgcca ggtcgttgga aaaatagccg cgcagaaaac cgaacatgca
    12241 aattcctgtc tcgctgatgc cgggcgcgtg agcgcgccgc ttggggatgc cgctcaaggg
    12301 cggcaggcga ccaggtggcg gtcgggagag agggaagaat aacagcttcg gacggaccgc
    12361 tacttggacg ggcgtacccg cagcgcgctg gcgccgtgct gcaccgcagc gtgcgccgtc
    12421 gcacacactg tcaaagggcg tcggtgcatg ctcctgtcat ccgcaccagg ccgcgccggc
    12481 ggcctcccat ggtcggcgtt tttcagcgtc gactggagcc agggcaaagc aagcggcaga
    12541 gcttaacgtg aggcaacatg gacgagacaa cacagacgac gcgatgctac gacatcatca
    12601 gcgacgaagc cgaatttcgc gctttgcagc ctgaatggga cacgttgtgg tcgaaggcga
    12661 acggctacta ctatcagtcg ttcagctttt gctggctggc ctggaaacac gtagcgaagc
    12721 cgcaggggcg caagctcaaa tgcatcgtgt gccgtgaaga cgggcaactg gtcatgatct
    12781 ggccgctcga aaccagcaag cgtgcgctct ggacatatct cgtgccgctc ggacctcaag
    12841 gcggcgacta cacgagcgtg ctggtggaga acggtgcggc ggctccggcc ttgatcgagg
    12901 gcgcgtggga gaccgcgcgc cggcgctgcg gcgccgattt cattcatctg ccgtatgtgc
    12961 gggatacgtt gcacctgcac acgctggtga tgcaggaacg cagggtgttg tttaccgagg
    13021 cccacaacgc gtctgccgcg acgttgcgcg gccagggcag ctgggaggaa tatggccgca
    13081 cgctaggcac gctgttcggc aaacggcccg ggtctttcgt gaaccgcctc gccaaggagg
    13141 gcaaggttgc cgtgcgcgtc gtcgatcctt ccgaggaagc cgagaccgca tccatcatcg
    13201 cgtggatgtt caattgcaaa cgccagtgga gcgaacgggt cggcaaacac agcgtgtgga
    13261 tcgatttgcc cgagttcgag tgctttctgg gcaagctgat ctattcgcct gatgtgccct
    13321 cgatggcgcg tctgatcgtg gtcaccctgg acggcgcgcc agtggccggg atcatcgtca
    13381 gtttcggcaa cccctgggcg agcgcgatct tcgccggtta cgacccgcaa tacggcaggt
    13441 gctgtcccgg tctgatcgcg gtggagcatt gcgtcaaatg ggccttcgac aacggcttcg
    13501 acctcgactt cggagtggga accgagcgct tcaagtcgta ctgggcgcgt ggggaagcgt
    13561 ccaccgcgtg gaccgtgcag atcatcaatt cggtgtgggg cctggcggcg attcgcgcgc
    13621 ggcgcctctc ccgcgagctc gtctcccgcg tgaagcgaat ccgccaggct ggtgccgcag
    13681 cgccggaggc gggcatcgag gacttgccgc tgacgccggc gatgaccctg cagagctcgg
    13741 aagcgttcgg ccagcgtcca gagcacaact gactgcaatt gaactgcgac tgaatcgcgg
    13801 cttagctgaa tgcaattgaa ctgcaactga atcgcggctt ggctgcaatg agccgcgacc
    13861 gaactgcaat tgtgccgcga ctgacgcaca acggagtcgc gtccgaaccg gctgcggcca
    13921 ttccccgttg cctggcttat gaccggtcgc ggttacgtgc ccgggtggcg cctgtgatca
    13981 cggcaaggcc gagcatgatg accgccacga tcgcgatcgt atgttgtgcg acatgcagcg
    14041 ccatcaagcc ccacgccacg ccgcctatca gaatgatcca gcctgccaga taaatgagca
    14101 gagtcatcgt ttttctcctt ttcgattcga cggatgggac cggattgcct gtcatgaaag
    14161 gcgttaccgg tcgccccgat tgggccgttg aagtcacgtg gccggcgcgc cgttgtagaa
    14221 cggcgccagc acacgcgatc agtgggtcta tcgagtagca gtttccgcgc ccggggcgct
    14281 gcatcatcga tgcgcaggcg ccgggagtcg aatggattcg aacgatacgc aacacgcact
    14341 ccgcgttaga tcaccttgat cacgacatcg atattgccgc gtgtcgcctt ggagtacggg
    14401 caggtttggt gcgcggcatt cgcgagcgct tcggcggctt cacggtccag acccggcagg
    14461 ctgacgttca gacgggcttg caggaagtag gcaccgtcgg tcgtgcccag gtcgacctcg
    14521 gcgtcaacgg cggtgtcagc gggcagggca atcttcagct tggcggccgc cagtcccatt
    14581 gcgccgatga aacaggcgga ccagcccgcc gcgaacattt gttccgggtt ggtgcccgat
    14641 cccggcgtgc ccggggtcga gagcttgacg tcaaggcggc cgtcggaact gcgagatgcg
    14701 ccttcccgac cacccgtcgt gtgggttttg ccggtgtaca gtactttttc gagctgcgtc
    14761 atgattgact ccttctttgg ttgagtttag tgatgcacgt agaaagatcg agcggaaact
    14821 gccgtgacag ggccacccga ttgggagacc gtctcccgaa tgccgccaaa cgactgcatc
    14881 gcggcgttcg cgtttgcatt agcgtctgcg tattccgcgc ggaccgtcag ggcgctttga
    14941 ccgcgttgcg aagcaggtgc gccggcaatc gggttgtagt ggggtgcggg accatatcca
    15001 ctgctggcaa agaccggtgt ggcgatggct gtgccgactg cgaagattgc tgcaacgatc
    15061 agatgctttt tcatggtgaa acctcgaatg agttttgttg catcaggcca ctcgtctctg
    15121 cgagtgaacc tgatatcaat aggatggctt attacgctcg aaggaaaatc actccttcgg
    15181 acgagggatg cctagctacc ccgatacaga ttgctgttga gatagacgag ctgtccgtca
    15241 tgtctggccc gctccagttc gtgtcggacg tctgcccggg ttttcaacac gacgcccttg
    15301 ccgctgccgg cagcgctcca ctgctgcgat tgcacgacgc caggatctgc tgcatttgtg
    15361 gccgccgttt cagaggcgtg agcgagaccg gtggtgacgg acgcggcgag tgccaaagcg
    15421 aggatggaag ttttcatgtc attctccgta atctaaagat gggaaaggcg catggacctc
    15481 agggagctag catggccgcc tgatctgcat ctgcggtatc ggatgcctgt cgtcttgcgt
    15541 tggatgcatt agaacgttcc gacgtatctg gcttgtgtcg aaaaggcggg caaatgcaat
    15601 gttgtgtatc gctgagacgc tcagatacat tgcgacacaa agcagccgtt cgaagaaccg
    15661 gagaaagatt ccgaccccga aggaacgcgc aaaagtgaat gagggagttg aatctgccga
    15721 tgccggagga gggccggctc cagctacgca agccgccgta cagcgaattg acggtgcgca
    15781 aactgtgcgg cctgcggttt gggctttatg cgtcgcctga ctatctcgaa cgcaagggcg
    15841 gggtcaagac gctcgccgag ctcaccaacc atgacgccat tgtgtttcgc ggatcgagcg
    15901 ggcgtctgcg atcctggagc gtgcgcgacg gcgctcggtg cgcgaggtgt cgctcccgcc
    15961 ggtgctggcg ctggccgatg gtcaggccct ggtggacgcg acagttgccg gcttcggtat
    16021 cacacagata cacgaccggg tcgcgctgcc gcatgtgcgc tcaggcaggc ttgttcctct
    16081 gctgccggaa gcggatgtga acggaccacc ggtccatgcc atcatttccc cgggtcagaa
    16141 aatggcggcg aagacgcgtg cggtggtcaa tcatcccgcc gagacgttcc agcagaccgg
    16201 gcagtaaagg acatcatccg cgcaatcgaa acgtcaaggc ctcgacatgc gaatgaatca
    16261 ggcatagctc ccgggcaccg acttcagtgt cgaggcgaga atcatggcgt cgctgttcaa
    16321 cggttcgatg cgtccccggg tgcgcggctt gtagaccgtg tcgcctctgc tgatgcgcga
    16381 gcctgacagc gcgcatcgcc cagagtgccg ggcgagcgcc ggcgaccata tctgctcttc
    16441 gtaattgcac atggtcgggt cgtgccagga gattacgacg agcttgttgg gtaagtactc
    16501 gagcacgctc accgtaaacg tgggctcttt ctggcgggca gggtctggcc gcgactgcag
    16561 tctcgagtac atgagctcat cagggaactc gcgaggcgca gcattgaact gctggagcgc
    16621 ggcgagtgtg cgactccagg ggtccgggtc gaccggcatc gcctcatggc gtgccgactt
    16681 gcgccggccg actctgccca tccccgcgga gacggggagc gggcgcgtgg accccacggg
    16741 cgccacgttc tggcgcgcag gggaggaata cgaaatatct gtctcggatg aaagttcgat
    16801 gggtgcatgg cggggcttat cgttcaacat catcttcctc ttgttagtaa gagtcgaagc
    16861 agtgattgag caaccgtggg gcaagggcac acgggcctac tggatgcaaa ccgcggcgta
    16921 gtcgaccgtg cgccggtaga cgccccggtt aagccacgcg acaaacgtga attccacgta
    16981 ggcgttgaca ccgatgaaga gcaaggtctg gagagcgtgc gacacggtca attcctttgg
    17041 agcgatgcgg cagttaaagg aaatacggtc aggccgacgg attgcgggcg tagaaaggcc
    17101 ggctgccggg tcgcggttcc gcacagggga tgaggactgg gttatcggga gaggcttccc
    17161 gcagtcatcg gagatgcgcc gatcgcgatg actcaagcgt acgcagcgca ggagagggaa
    17221 tctattctac ggtggggtat gggagtgccg cgatagcgta atccggctat acggcggtat
    17281 aacgcaggga ggccggcatg agcaggttgt cgcctccacg agcggcccgt tatacattcg
    17341 tatcatcgcg gtcgtcggtt ttgtaagcta ggcttggtat gcaggataaa cgcgcggaat
    17401 gccacgccgt tattcaggga gcgagccgag gaccaccatt ttgcaaaaga cgcccgttgt
    17461 atcaattgtc gacgatgacc agtcggttcg tcgtgcgctg ggcagcctcg tgcgatcgat
    17521 gggctgggaa gcgcgcatgt tcgagtccgc ggaagcgttt ctcgcatcgg gggacgtgtc
    17581 ggacacggcg tgtctcctgt cggacatccg catgtcggga atgtcgggcg tagagatgca
    17641 tgagcgtctc gtggcgcaag ggcaagcgcc ggcgaccatc tttatcagtg cctaccctac
    17701 gccatcgtta caggagaagg tgacgggcaa cggcgccctt gtgctgctgc aaaaaccctg
    17761 tcaggtgtcc gtcatgacac attggctctc ggtggcgctc ggcgcccagt agccgcgctt
    17821 aagagaagtc tgtactccag caccttattc ctttggtgcg atacctcgac cagcaaacga
    17881 gaaaaaaaag ccccgcgtag cggggcgaaa atgcgcacag gggtctgcgc agaggagttc
    17941 agaaggacga tgactcagta tgcatgcagt cagaaaaaca cgtccgcttc gtgtacagct
    18001 cacctgccca cgctcacctc cggtgacttt tcctcacact accccagcct taatatccat
    18061 tgagtcgcgc ccagccgact cagataattt gtccaccaga tttgccaacc gcgatcgacc
    18121 cgcactgccg cgcgctccga catgcccgcg ccatcctggg ggcgatcgcc acatagaaag
    18181 gagacaaggt ggaacaacag agcgaaagcg ggcggggcag ggttgcggtc gtgaccggcg
    18241 gtgcaatggg tattggtgcg gatgtggccg aaagacttgc gcgcagtggc taccacgcgg
    18301 tgctttccga catcaatcgc ggcgccgcgg acgaaacggc cgggaagttg cgcgcgcaag
    18361 gactctccgc ttctgctgtg gtgatggatg tgggcaatcc cgaatccatc gccgaaggct
    18421 ttgccgccat cgcgcgcgac gccggccgtt gcgacgtgct ggtcaactgc gcgggcatcg
    18481 caacgatgct tcctttcatt gattatccgc tcgacgtgta tctcaagacg atgcaggtca
    18541 acgtgaccgg cacgctgctg tgcgcgcagc acgcggcccg gatgatgagg ccgaacggct
    18601 gtggacgcat cgtcaatatc gcgtccgtgg cgggcatgcg cgcggtcggc accggccgca
    18661 ctgcgtacgg cacatcgaag ggcgcggtga tcgcgctgac ccggcagatg gctgtcgagt
    18721 tgtgcgacta cggcattacg gcgaatgcgg tctcgcctgg tcccgtcgat acgccgatga
    18781 caaaggcact ccattccgcc cagttccgcg aagagtattc gcgtgcgatt ccgctg
//
LOCUS       DATASH010000831         4829 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1328857,
            whole genome shotgun sequence.
ACCESSION   DATASH010000831 DATASH010000000
VERSION     DATASH010000831.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4829
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1328857"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            234..659
                     /locus_tag="VHV99_22320"
     CDS             234..659
                     /locus_tag="VHV99_22320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638162.1"
                     /translation="MQKLILPFVAGFLASLFFHESTLALLHAAGLIDPNGFSTAPFLP
                     LGLPEFIANAIWSAFWAVLMAWLLRVAPGRRAPWVPAFVFGGIAVTAASVFVVDPLRG
                     IWPSGNMLPRLAVGFAANAMWGWGALVFMRAFMASEDED"
     gene            complement(799..1935)
                     /locus_tag="VHV99_22325"
     CDS             complement(799..1935)
                     /locus_tag="VHV99_22325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX3638163.1"
                     /translation="MSISASPAVSDEVATYVANRIGFIELERPKALNALSTGMIRAVH
                     AALDQWRENPDVLAVVVRSAHPRAFCAGGDVRFLYESAERGEQDARDTFFIEEYRLNH
                     AIFTYPKPYIALTNGVVMGGGMGISQGAHRTGGLRVVTNSTKMAMPETRIGLFPDVGA
                     SWFLARTPGALGRYLAVTGETIGAADALYAGLADAYIADAALPALVDTLRSELFERGA
                     DVVACVERETAAHQVVPQPDASPLASTRALIDRHFALPDMTRILASLEQARAHGGETA
                     DWAEQTIAVLRERSPLSMAVSLEVVTRAEGSMADVLRGDLDLTRTSFAQGDTIEGIRA
                     RIIDKDNAPRWRFARIEDVSAAEVAKMFESPWPANEHPLRDLRA"
     gene            complement(1982..3151)
                     /locus_tag="VHV99_22330"
     CDS             complement(1982..3151)
                     /locus_tag="VHV99_22330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX3638164.1"
                     /translation="MGIVERKSRQKQALRERILDAARRIVMREGFAALSMRKIADAIE
                     YSPATLYLHFASRDEIAQALCAEGYAQLLETFVPLAQIADPAERLRALGRAYVAFGVA
                     HPETYRLIFMEDPSYTGAALGGMAAMSGTGVSVAANADAGTMDEAVAMPAARAAAAAR
                     AAATGTAAAETTAAETTAAEATAAEATAVGSHASGKASLAPAKSDDDPGDASLRIMLS
                     ALEELKAAGRLSASMDVAVWAEAFWATLHGIVALNLTCPVFPTAPLDTVVGVALDAWL
                     GTQGAGQPPRLTRSFRARKEKMPAEAPVESASELGGELAAQPSRPSAPSGKKFTKRPE
                     PSALKPATDADTHDPTKSATKSATKPATKPETKPETKPETKAPTRSATRPKATRA"
     gene            3306..4460
                     /locus_tag="VHV99_22335"
     CDS             3306..4460
                     /locus_tag="VHV99_22335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX3638165.1"
                     /translation="MQATGKVGLFGAAGAAGQIIAAALSAAGRDYRVVGRARAPLEAA
                     FGHDPHAEIVTWNPDDPASISAAAAGLQTVIYLVGVPYDQFAAHPPLMERTLSGVTAA
                     GVERFILIGTVYPYGRARGNPISENHPREPHTFKGKMRLAQENLVLAAHGRPSGSASG
                     SASKSASEGACGSASKSAREGASGSASEGASGSTSGRVSGGVSGSSRLSTLVLRLPDF
                     YGPGIERSLLNGVFEAAAHGKRAQVIGPVDVPHEFIYLPDIGPVVEKLTRTPEAYGRW
                     WHLAGAGTITQREVARQAYALAGREPKLMVAGKIMLRMLGLFNPLMRELVEMNYLMTE
                     PVVLDDAALTQLIGPIAKTSYEDGIRQAFDAARATASAGVDPHRDAAKTA"
     gene            complement(4480..>4829)
                     /locus_tag="VHV99_22340"
     CDS             complement(4480..>4829)
                     /locus_tag="VHV99_22340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HEX3638166.1"
                     /translation="VRGWPVLTGVPILPDTSDEATMEIRFPADAPAYRDSNLTVVFPA
                     LVDGEPVPCAISVEALEDHFGAHTEDLEGWMRAFDAGRPCIEAVAREHLQISHGTPVL
                     LKSGHFPPGNVAV"
BASE COUNT          836 a         1516 c         1559 g          918 t
ORIGIN      
        1 gcggcaaacg ccgtctgcat ggagaaacag tctatggatc ggggcacggc ttttgaacga
       61 ttttttttag cttaggtttt ccactttcgt gattagcccg acgctaaagc atacgggtgt
      121 tgcaccttcc tcacgctgga aacggggtcc ccctctatat aatggcgcca cgtttccaat
      181 aatccgatgc aggccgcgcg aaccacgccg cctgagctct cgcggtgcaa tgcatgcaaa
      241 aactcatcct gccgttcgtc gccggttttc tggcttccct gttctttcat gaatcgacgc
      301 tggcgctgct gcatgcggcc ggtctgatcg acccgaacgg tttttcaacc gcaccgtttt
      361 tgccgctcgg gttgcccgag ttcatcgcga acgcaatctg gagcgcgttc tgggcggtgc
      421 tgatggcgtg gctgctgcgc gtggcgcctg ggcggcgtgc gccgtgggtg ccggccttcg
      481 tattcggcgg cattgcggtg accgcggcga gtgtgttcgt ggtcgatccg ttgcgcggca
      541 tctggccgag cggcaatatg ctgccgcgcc tcgcggtggg ttttgcggcg aacgcgatgt
      601 ggggttgggg cgcgctggtg ttcatgcgcg ccttcatggc gagcgaagac gaggactgaa
      661 cgttttcagc tccgctcgcg agactatgct ggttcggtgg tttgcggttt gcggttctgc
      721 ggttctgcgg ttctgcggtt ctgcggaccg gcgggcagca tagcttctgc cgctgctatc
      781 gctgttgccc gagcgtgttc aggcgcgcag atcgcgcagc gggtgctcgt ttgccggcca
      841 cgggctttcg aacatcttcg ccacctcggc ggcgctcacg tcttcaatgc gcgcaaaacg
      901 ccagcgcggc gcgttgtcct tgtcgatgat gcgagcgcga atcccttcga tcgtgtcgcc
      961 ctgcgcaaag ctcgtgcgag tcagatccag atcgccgcgc agcacatccg ccatcgaacc
     1021 ctccgcacgc gtcaccactt ccagcgacac cgccatcgac agcggcgaac gttcgcgcaa
     1081 cacggcgatc gtctgctcgg cccaatcggc ggtttcgccg ccatgcgccc gcgcctgctc
     1141 cagcgaagcg agaatccgcg tcatgtcggg caacgcgaaa tggcggtcga tcaacgcacg
     1201 tgtgctcgcg agcggcgaag catcgggttg aggcacgacc tgatgcgcgg cggtctcacg
     1261 ttcgacgcac gcgaccacgt cagcgccgcg ttcgaacagt tcgctgcgca acgtgtcgac
     1321 tagcgcgggc aaggccgcgt cggcgatata ggcgtcggcg aggcccgcat agagcgcatc
     1381 ggcggcgccg attgtttcac cggtgaccgc cagataacgg ccgagcgcgc ccggcgtgcg
     1441 cgccagaaac cagctcgcgc cgacatcggg aaagagtccg atgcgggtct cgggcatcgc
     1501 catcttcgtc gagttggtca ccactcgcag gccgccggta cgatgtgcgc cctgcgaaat
     1561 ccccatgccg ccccccatca cgacgccgtt cgtcaacgcg atatagggct tcgggtaggt
     1621 gaagatcgcg tgattgagcc ggtattcctc aatgaaaaac gtatcgcgcg cgtcttgctc
     1681 gccgcgctca gccgactcat acagaaagcg gacgtcgccg cccgcgcaga acgcgcgggg
     1741 atgcgcgctg cgcaccacca cggcgagcac gtcgggattt tcgcgccact gatcgagcgc
     1801 cgcgtgcacg gcgcggatca tgccggtcga cagcgcgttg agcgccttcg gcctttccag
     1861 ttcgatgaaa ccgatgcggt tggctacgta agtcgcgact tcgtcgctga cggcgggcga
     1921 ggcggaaatg gacatggaga gctaagcgcg atggcgcgag atgagtcgga actgaacgtt
     1981 atcacgcacg cgttgctttc ggcctggtgg cggacctggt cggcgctttg gtttctggtt
     2041 tggtttctgg tttggtttct ggtttggtcg ccggtttggt cgccgatttg gtcgccgatt
     2101 tggtgggatc gtgggtatcc gcgtcggtgg cgggtttgag ggccgatggc tcaggccgct
     2161 tcgtgaactt cttgcccgac ggcgcggagg gccgagatgg ctgagcagcc agttcgccgc
     2221 ccaactcgct ggccgattcc accggcgctt cagcaggcat tttttctttg cgcgcgcgga
     2281 aggagcgtgt gaggcgaggc ggctgccctg cgccctgcgt gcccagccac gcatcgagtg
     2341 cgacacccac caccgtatcc agcggcgcgg tcggaaacac cgggcatgtg aggttgagtg
     2401 cgacgatgcc gtgcaacgtc gcccagaacg cctcggccca cactgccacg tccatggatg
     2461 cggacaggcg ccccgccgct ttcaattctt cgagcgcgga gagcatgatg cgcaacgaag
     2521 cgtcgccggg gtcgtcgtca gatttcgcgg gcgcgaggct cgcttttccg gaagcgtggg
     2581 aaccgacggc cgttgcttct gctgccgttg cttctgctgc cgttgtttct gctgccgttg
     2641 tttctgctgc cgctgttcct gtcgccgctg ctcttgctgc cgccgctgcc ctagccgccg
     2701 gcatcgccac cgcttcgtcc atcgtcccgg catccgcgtt cgcagccacc gacacaccgg
     2761 tccccgacat cgccgccata ccgcccaacg ccgcgcccgt atagctcgga tcttccatga
     2821 agatcaaccg ataggtttcc ggatgcgcga caccaaacgc cacataagcg cgcccaagcg
     2881 ccctcaaccg ctcagccggg tcggcgatct gcgctaacgg cacgaacgtc tccagtaatt
     2941 gcgcataacc ttccgcgcat aacgcctgcg cgatctcatc gcggctcgcg aaatgaaggt
     3001 acagcgtggc gggcgaatac tcgatggcgt ccgcaatctt gcgcatggac agcgcggcaa
     3061 agccttcgcg catgacgata cgccgcgcgg catcgaggat gcgctcgcgc agcgcctgtt
     3121 tctgacggct ttttcgttcg acgattccca tgacgaacat tctcccgttg acaaactgca
     3181 aagtcaaatt aaactgaaca gtgttcacta aacggtgttc attaaatttt cgtcggccat
     3241 tccagatcgt tgaagattag tcatcgctac ccgggcgcga tacggcgccg cctccaggag
     3301 tcgtcatgca agccacaggg aaagtgggat tattcggtgc cgcaggcgcg gccggtcaaa
     3361 tcatcgcggc ggcgctgagc gcggcgggcc gggattaccg cgtggtcggc cgcgcccgcg
     3421 cgccgctcga ggcggccttc ggccacgatc cgcacgcgga aatcgtcacg tggaatccag
     3481 acgacccggc ttcgatcagc gccgcggccg ccggcttgca gaccgtgatc tatctggtgg
     3541 gcgtgcctta cgatcagttc gccgcgcatc caccgcttat ggaaagaacc ctcagcggcg
     3601 tgacggcggc gggcgtcgaa cgcttcattc tgatcggcac cgtgtatcca tacggccgcg
     3661 cgcgcggcaa tccgattagc gaaaatcatc cgcgtgagcc gcatacgttc aaaggcaaga
     3721 tgcggctcgc gcaagaaaat ctggtgctgg cggcacatgg acgtccaagc gggagtgcaa
     3781 gtggcagtgc aagcaagagc gcaagtgagg gcgcatgcgg cagtgcaagc aagagcgcaa
     3841 gggagggcgc aagtggcagt gcaagtgagg gcgcaagcgg tagcacaagc ggccgtgtaa
     3901 gtgggggcgt aagcggatcg agccgcctgt cgacgttggt gctgcgcctg ccggactttt
     3961 acggccccgg cattgaacgc agtctgctca atggcgtgtt cgaggccgcg gcgcacggca
     4021 agcgcgcgca ggtaatcggg ccggtggatg taccgcacga attcatctac ttgccggata
     4081 tcgggccggt ggtcgaaaaa ctcacgcgta cccccgaagc atatggccgc tggtggcacc
     4141 tggccggcgc gggcacgatc acgcagcgcg aagtcgcgag gcaggcctat gcgctggcgg
     4201 gccgcgagcc gaagctgatg gtggccggca agatcatgct gcgcatgctc ggccttttca
     4261 atccgctgat gcgggaactg gtggagatga actatctgat gaccgagccg gtcgtgctcg
     4321 acgacgctgc actgacgcaa ctgatcggtc cgatcgccaa aacttcatac gaagacggta
     4381 tccgtcaggc gttcgacgct gcgcgcgcaa cggcatcggc gggtgtggac ccacatcggg
     4441 acgccgcgaa aaccgcctga ccgagggcag ccttcgcgtt taaaccgcga cgtttcccgg
     4501 cgggaaatgg ccgctcttga gcaacacagg cgtgccgtgg ctgatctgca aatgttcgcg
     4561 cgcaaccgcc tcgatacacg gacgcccggc gtcgaacgcg cgcatccagc cttcgagatc
     4621 ctcggtgtgc gcgccgaagt gatcctcgag cgcttcgacc gagatcgcgc acggcaccgg
     4681 ttcgccgtcg acaagcgcgg gaaacaccac cgtcagattg gagtcgcggt atgcaggcgc
     4741 gtccgcggga aaccgaattt ccatggtcgc ctcatcagaa gtatccggca ggatagggac
     4801 accggtgaga accggccacc cccggacat
//
LOCUS       DATASH010000832         8362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6271208,
            whole genome shotgun sequence.
ACCESSION   DATASH010000832 DATASH010000000
VERSION     DATASH010000832.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 8362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8362
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6271208"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(418..657)
                     /locus_tag="VHV99_22345"
                     /pseudo
     CDS             complement(418..657)
                     /locus_tag="VHV99_22345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000796.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1653..1856
                     /locus_tag="VHV99_22350"
     CDS             1653..1856
                     /locus_tag="VHV99_22350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015004218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX3638167.1"
                     /translation="MQTGTVKWFNDAKGFGFIAPDDGGEDLFAHFSEVKSDGFKSLSD
                     GQKVSFDVKLGPKGKQAANIQPL"
     gene            complement(1965..2264)
                     /locus_tag="VHV99_22355"
     CDS             complement(1965..2264)
                     /locus_tag="VHV99_22355"
                     /inference="COORDINATES: protein motif:HMM:NF019984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron maturase-specific
                     domain-containing protein"
                     /protein_id="HEX3638168.1"
                     /translation="MARDHGVGGQVEPLATRWANYFKVGSDSRAYRALDSYTATRLRR
                     WLRNKYKVRRSRGGSYPSSHLYGHFGLVRLTSTDKRQSRHPDSGRSRTRRCVHRR"
     gene            complement(2192..2755)
                     /locus_tag="VHV99_22360"
     CDS             complement(2192..2755)
                     /locus_tag="VHV99_22360"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HEX3638169.1"
                     /translation="MQHLIRMWLECPVEETDSRGRKKRTTEPRDSRRGTPRGSPISPL
                     LANIYMRRFVLAWKKLGLQRSLGRRIVTYADDLVILCKRGKAEEPLSRMRAIMDKLKL
                     TVNEEKTRICKVPEGEFDFLGYTFGRLYSATTGQARMGMRPSKKSIRRMVEKIHAMTA
                     LETAWQETTELVGRLNRSLRAGRTTSR"
     gene            3010..3411
                     /locus_tag="VHV99_22365"
     CDS             3010..3411
                     /locus_tag="VHV99_22365"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX3638170.1"
                     /translation="MSTAPAFWMIPSAPTPVGPFSHATETDGWVFLTGQMPTSPTDDA
                     APLPDGVVAQTQRVMDNLILVLQGVGLELRHVVAVRIFLTEFKRDYAAMNDTYRAYFP
                     ADALPARTCIGVTALARDALVEIDFIAKRPD"
     gene            complement(3431..6994)
                     /locus_tag="VHV99_22370"
     CDS             complement(3431..6994)
                     /locus_tag="VHV99_22370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3638171.1"
                     /translation="MQKILDRTQESLAHAFASLAQSAKRQQRLYLATIGSLMLIVVIF
                     TLLLAVLAATKQLDYRRALATQNAADIRALLHREQAFLARAELTLAYYNAFEGQLPLP
                     EGMAESVQKTGAARSLVEAAGAHIDLLIGDATRAAWGPQMNTRLWRLYQTGVSTLATE
                     QAFALRQRAMLVGLNEDYAVMLPAADAPGATSGSDALLLQPATIGTLRDTIERQLQAQ
                     TGKRVPGKGERIWLGPYRDPLQGVPVITAVSAYYSGGTPVTLITTSIPVSTLPDYFTR
                     PAAEGTLLITTPDRRVIVSSPPVDAGTSHMLSNLVANIPADGYRYTRDGAVLVEPLAP
                     DFGSLVGYLPWRALVGALRWQLATLAGLALLVLGSIALTARLWGLRLLQKTFAETTRA
                     LESETINHILVSATPIGLCIVRQSDFSILTANALADELLHIEAGSNRLPPHIVGEFSA
                     QAPDKPSLTAFAKVAAFVAPARPLPSQSQSHLLQPVTQAATQPVTQPQPDQGEAAPAQ
                     FLQFTYAAARYADEDVLFCAILDVTAQHALEQQLRLAQQTSESMMRARSNFFASMSHE
                     IRTPLNALLGNLELFARTPGLEAHTQRLATLEVAAGALRRIVNDILDFSKIDAGEMRL
                     MAESFRPIDDFENIALSYAPMTADRAIRFYSHLSPTLDQTLRGDRTRIAQIVNNLLSN
                     AFKFTSCGKITLSAEVKDDLQGRPILTCRVCDSGIGMDQALVARIFNPFEQGEASTSS
                     RYGGTGLGLSICARLCELMGGHITVESVLGVGSAFSASIPLALPPDELRVPAVEPTRR
                     GSVLVLCQERESGQLLDGWLQRAGWSAHTVTSTRSALAWLRVNRPQAMLITGEYSLDA
                     IAGLRAVQPVGAAWITRTGPHRPAARGEGVLEATEFSRTAILSAIELAAEGGAAADSA
                     EALAGEIAEALPPAVDPALHGLTVLVAEDNPLIQTLIVEQLDALGCLPTIAGDGRQAL
                     AAFELGHFEVVLTDIHMPVMDGYGLLAALRKAHADLPVLAFSAVTDNQQRKGWRERGF
                     TGYVAKPASLGELETALLAVASAGLRTGTSASKVTAAIAPVAPTTSAAAGASADPANM
                     LSADDKTRYTAMLKQHLQTDLPRLMAIVEQEDRKALGGWAHSAGGAFLVVQERQFAQQ
                     CRELQRLCENSEAWTTEMDERAISLHEAICDQFGLDEASMH"
     gene            7240..7583
                     /locus_tag="VHV99_22375"
                     /pseudo
     CDS             7240..7583
                     /locus_tag="VHV99_22375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490401.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
BASE COUNT         1639 a         2650 c         2532 g         1541 t
ORIGIN      
        1 ggatggcctg caacatatcc atcgatcgct ttccgttcat cggggttaaa aatgcgtcaa
       61 tgtctggcaa ccagccagtc atgccgcgcc gcaatatgcc tacggggcac aagatgaatc
      121 gaccttgaac tcggcgggtg cgcagatatg cgcgagcgcc tttccatagg cgcaccgcgg
      181 aacgttgcca tatattactt cggcgcaaag agggccgggg ttgcgagcga ctaaaagcgg
      241 caggaaaacc cgagatgcgt tcgatccgag gtgttcgtag ctgagctgcc cacgcgagac
      301 tagcgttcgg ccgggcccga cgcggacctc gaacgaacga cgcgcgtgat gaccatcgcc
      361 accacaagac gcgtgtcggt ttgtcgagta ttgcagcaag aaacggggag cgggtgcatg
      421 ggcgatcgat ttttccgtca ccgttgtgtc gacaagcgcc cgcttcacgc tcgcggcctt
      481 gatcacactg gcacgtttcg ctgcttcgat cgtctcggcc agctgctctt caacgccggc
      541 ctcgcccggt cgtttgcgcc agcgcgtaag acttgacggg tcgatcggcg gttcggtctg
      601 caaataggtc tcgccggtga aaaccttcca gtacgggttc tctacctatt gccagacgaa
      661 tggttctata tgggactgag ccttcacaat ctggcctcgc ttatatgagt cgtcgcctac
      721 catcctcttc tccataaaat cgtcgaaagc cggtccgtag caaagtggcg ttccgggctt
      781 tcaggctcga tttcggacga cagctaacgc gcaacgtggc actacattcc gtcagtacta
      841 caggataaaa acgagaacta aaaatccggc ttccggaaca ccttttccag cgtcagtatg
      901 gcttcgataa atcatataaa tgtggcacat agtttgagcg gtaagagatt gcgaattggc
      961 gggtaaaccc gattttaagg ggggtgatgt ttcctgtatc ttgttcatta cgtttataaa
     1021 gacatactga acatctgact aatagcaccg gactgagagg atgacgcctc ttgtgcagaa
     1081 tgccattatt cagacatata tgacggagag agaacacagt gaacgttgcc accaaagcag
     1141 acgactggac gagcccctac acccctcaaa accttgactc tgctgttgca catcttgagc
     1201 gggtcctatg cacagaaggt gcagattcat tattcgccca gacttactgg cgcgctcggg
     1261 ttcttcaggc gtatgcgacg cccggactcc tgcaacgaca acgggaacgg ctactgcgat
     1321 tgctcgaccg tctcgccagc gctcccacgg attcgagacg tcggatgcag accgcctgac
     1381 cttggtccct ccgcctagcg gcatctgacg ggcatagact tggaacggct gacttgcgca
     1441 cagaagcagg tggcacagat gcttgccctt cgcgctccat cgcagcaggc ttgcaaaccg
     1501 aacaaccatt cgcaggccag atactgtttt tacggggaag cggtaatagc ttgtcctccg
     1561 ggtgtccgca cgcctgacca gcgccttcag aggcactttt agagtttttc agcgagtcaa
     1621 tgactctttt ttaacttcct tggaatcaga tgatgcagac gggcacagtg aagtggttca
     1681 acgatgcaaa gggcttcggt ttcatcgcgc cggacgatgg tggcgaagat ctgtttgccc
     1741 atttttccga agtgaagtcg gacggcttca agtctcttag cgatggccaa aaggtcagct
     1801 tcgacgtcaa gctcggacca aagggcaagc aggccgcaaa tatccagccg ctctgagaac
     1861 ggcccggttc atgccgcacg catagtgcgg tatgaaaacc cacgcgagat aaccacacca
     1921 ttatctaaat ggacctgtca gcaagcgcat tcgtaaccgg gacctcacct cctatgaacg
     1981 catcgccggg tacgtgaacg accgctgtcg ggatggcgcg actgcctctt gtcggtagag
     2041 gtcagacgca cgagaccgaa gtgcccgtag aggtgtgagg atggataact cccgcccctc
     2101 gaacgtcgaa ccttgtactt gttgcgcaac caccgacgca accgcgtagc ggtgtagctg
     2161 tcgagtgccc gatacgcgcg gctgtccgag cctaccttga agtagttcgc ccagcgcgta
     2221 gcgagcggtt caacctgccc accaactccg tggtctcttg ccacgccgtc tcgagggcgg
     2281 tcatcgcatg aatcttctcg accatgcgcc gaatactctt cttcgacggg cgcatgccca
     2341 tgcgggcctg cccagtcgtc gcagagtaca gccgcccaaa cgtgtaaccc aggaagtcga
     2401 attcgccttc cggcaccttg cagattcgtg tcttctcctc gttaaccgtc agcttcagtt
     2461 tgtccatgat cgcgcgcatt cgtgacaagg gttcttctgc cttgccacgc ttgcacagga
     2521 tcacgagatc gtccgcgtag gtcacgatac gacggccgag actgcgctga agtccgagct
     2581 tcttccatgc cagcacgaac cggcgcatat aaatattcgc cagaagtggt gagatgggtg
     2641 aacctcgcgg agtgccacgt ctgctgtctc tgggctccgt cgtacgtttc ttccggcctc
     2701 ggctatcggt ttcttcaacg gggcattcca gccacatcct gatcagatgc tgcacgcgcc
     2761 gatcaacgac ctgtcacgac cgtgatgtac tttccgtctg aaacagacga cagaaacaat
     2821 ccgtacccct cgcgtacaca gtcatcggca agctgtttca tcggatctgc gtacggaaac
     2881 tgatgcacgt tccgcaagca gcgtgcgtgc ggcgacgcga cccgccacga gcgaagtgcc
     2941 gcccgccaga cattttgatc ggttagctta gcgtcatcga caatcgaaac ccgctagcca
     3001 accatcccca tgagcaccgc tcccgccttc tggatgatcc cgtccgcgcc gacgccggtc
     3061 gggccgttct cccacgccac cgaaaccgac ggctgggtgt tcctgaccgg ccagatgccg
     3121 acctcgccca ccgacgatgc cgcgccgttg cccgacggcg tcgttgcgca aacgcagcgt
     3181 gtgatggaca acctcatact cgtgttgcaa ggcgtcggcc tggaactgcg gcatgtcgtt
     3241 gccgtgcgta tcttcctgac cgagttcaag cgcgactatg cggcgatgaa cgacacctac
     3301 cgtgcctact tccccgcgga cgcgttgccc gcgaggacct gcatcggcgt cacggcgctg
     3361 gcgcgcgatg cgttggtcga gatcgacttc atcgcgaaac ggcctgactg atccgccgca
     3421 acgcggcgca tcaatgcatc gacgcttcat ccaacccgaa ttgatcgcag atcgcctcgt
     3481 gcaacgagat cgcgcgttcg tccatttcgg ttgtccaggc ttcgctgttc tcacataacc
     3541 gctgaagttc acggcattgt tgcgcgaact gccgctcctg caccacgagg aacgcgccgc
     3601 cggcgctgtg cgcccagccg cccaatgcct tccggtcctc ctgctcgacg atcgccatga
     3661 gcctcggcaa atcggtttgc aagtgctgtt tcagcatggc cgtgtaacgc gtcttgtcgt
     3721 ccgcgctgag catgttggcc ggatcggcgc ttgcaccggc cgcagccgag gtcgtcggcg
     3781 ccaccggcgc tattgcagcc gtcacttttg acgctgacgt cccggtccgc aaccccgccg
     3841 acgccaccgc gagcaacgcc gtctccaact cacccaacga cgccggcttg gcaacataac
     3901 cggtaaagcc gcgctcgcgc cagcctttcc gctgctgatt gtctgtgacc gcgctaaagg
     3961 ccagcaccgg caagtccgca tgcgccttgc gcagcgccgc gagcaatccg taaccgtcca
     4021 tgaccggcat gtggatatcg gtcagcacga cctcgaagtg tcccagctcg aacgccgcca
     4081 gcgcttgccg cccgtcgccg gcgatcgtcg gcaggcagcc gagcgcatca agttgttcga
     4141 cgatcaaggt ttggatcagc gggttgtctt cggcgacgag aaccgtcagg ccgtgcagcg
     4201 ccggatccac agccggcggc agtgcctcgg caatctcgcc ggcgagcgcc tcagcgctat
     4261 cggcagccgc cccgccctcg gccgcgagtt cgatggcgga caggatcgcc gtgcggctga
     4321 attcagtggc ttcgagcacg ccctcgccac gcgccgcggg acgatgcggc ccggttcgcg
     4381 taatccacgc ggcgccaacc ggttggaccg cgcgcaatcc ggcgatggcg tcgagactgt
     4441 actcaccggt gatcagcatc gcctgcggcc ggttcacgcg taaccaggcc agcgcgctac
     4501 gcgtcgacgt caccgtgtgg gccgaccagc ccgcgcgctg caaccagccg tcgagcagtt
     4561 gtcctgactc acgctcctgg cacaacacca gtacgctgcc acggcgggtc ggctcgacgg
     4621 ccggcacgcg caattcatcg ggcggcaacg caagcgggat cgaagcgctg aacgcactgc
     4681 ccaccccgag cacgctctcg acggtgatat gtccccccat caattcgcac agccgcgcgc
     4741 agatcgacag acccaaaccg gtgccgccgt agcggctcga ggtgctggcc tcgccctgct
     4801 cgaacggatt gaaaatgcgc gccaccagcg cctgatccat gccgatgccc gaatcgcaga
     4861 cccggcaggt caaaatcggg cggccttgca gatcgtcctt cacttcggca ctcaacgtga
     4921 tcttgccgca cgacgtgaac ttgaacgcgt tgctcaacag gttgttgacg atctgcgcga
     4981 tgcgcgtgcg atcgccgcgc aaggtctggt cgagcgtcgg cgagaggtgt gaatagaagc
     5041 ggatcgcgcg atccgccgtc atcggcgcgt aggaaagcgc gatattttcg aagtcgtcga
     5101 tcggccggaa cgattcggcc atgagcctca tctcgccggc gtcgatcttc gaaaagtcga
     5161 gaatgtcgtt gacgatgcgg cgcagcgcac cagccgccac ctccagcgtg gcgagccgtt
     5221 gcgtgtgcgc ttcgaggccc ggggtccgcg cgaacagttc gaggttgccg agcagcgcgt
     5281 tcaacggcgt gcggatttcg tggctcatcg acgcgaagaa attcgaacgc gcccgcatca
     5341 tgctttcgga agtctgctgt gcgagccgca gttgctgttc gagcgcatgc tgcgcggtca
     5401 cgtcgagaat cgcgcagaac aacacgtcct cgtcggcata acgcgcggcg gcgtaggtga
     5461 actgcaggaa ctgtgcgggc gccgcttcgc cctggtctgg ctgcggttgc gtgaccggct
     5521 gggttgcagc ttgcgtgacc ggttgcaata aatgcgactg cgactgcgac ggcaacggtc
     5581 gtgcaggcgc gacaaaagcg gcgaccttcg cgaacgccgt cagcgaaggc ttgtccggtg
     5641 cctgcgccga gaactcgccg acgatgtgcg gcggcaggcg gttgctgccc gcttcgatat
     5701 gcaacaactc gtcagccagc gcgttggcgg tgaggatcga aaaatcgctt tgccgaacaa
     5761 tgcataaacc gatcggcgtc gcgctgacga gaatgtgatt gatggtttcg ctttccagcg
     5821 cgcgcgtcgt ttcggcgaac gtcttctgta acagacgcag cccccataga cgagcggtga
     5881 gcgcgatcga tcccaatacg agcagggcaa ggccggcgag ggtcgccagt tgccagcgta
     5941 gtgccccgac taacgcccgc cacggcagat aacccaccag cgagccgaag tccggcgcga
     6001 gcggctcgac cagcaccgcg ccgtcgcggg tgtagcgata cccgtcggcg ggaatattgg
     6061 cgacgagatt gctcagcatg tgcgacgtgc ccgcgtcgac cggcggcgaa gacacgatca
     6121 cgcgacggtc tggcgtcgtg atcagcagcg tcccctcggc cgccggccgg gtgaaatagt
     6181 cgggcagcgt gctgaccggg atgctggtcg taatgagcgt gaccggcgtg ccacccgaat
     6241 agtaggcgct cacggcggtg atcaccggta cgccttgcag cgggtcgcga tagggaccga
     6301 gccatatccg ctcgcctttg cctggcacgc gcttgccggt ctgagcctgc aactgacgct
     6361 cgatcgtgtc gcgcagcgtg ccgatcgttg ccggttgcag cagcagcgcg tcggagcccg
     6421 atgtggcgcc cggcgcgtcg gcggccggga gcatgaccgc gtagtcttcg ttcagcccga
     6481 ccagcatggc gcgctggcgc agcgcgaagg cctgctcggt cgccagcgtc gagaccccgg
     6541 tctggtacag acgccagagt ctggtgttca tctgcggccc ccacgccgcc cgagtcgcgt
     6601 cgccaatcag cagatcgata tgcgcgccgg ccgcctccac aagactgcgt gccgcgcccg
     6661 ttttctggac cgattccgcc atgccctcgg gcagcggcaa ctgaccttcg aaagcgttgt
     6721 agtaggcgag tgtcagctcg gcacgcgcca aaaacgcctg ctcgcgatgc agcagcgctc
     6781 tgatatcggc ggcattctgc gtggccaagg cgcgccggta gtccagttgt ttggtcgcgg
     6841 cgagaaccgc gagaagcagc gtaaatatca ccacgatcag catcagcgag ccgatcgtcg
     6901 cgagatacaa gcgttgttga cgcttggcgc tctgcgcgag cgaagcgaac gcgtgggcca
     6961 gtgactcctg agtacggtcg aggatcttct gcatcgtctc gcttgaggag cggattcgcg
     7021 caggcagacc gctgcggatg tcggggtgac gaaatgcgtc tgcaggccgt gtccggcgca
     7081 gtgtaacgcg tttgaaggta cggcggcatg ccatccggca ccgtttccgc ggccattcgg
     7141 cacgcatgaa acaaccctgt attcacggct ctgcacccgc taaacccgag tggtttactg
     7201 ggaaaacccg caagcgtgcg gggttaatgc cgctatcccc tcgacctcgc gcgccgcacc
     7261 gaattgcagg cggcctcgtt cgaggaaacg gcggccacca tggaggcgct gactaccacg
     7321 gtgcaggcca acgccgacgc cagcgtgcgc gcgctcgatc tggcgaagga cgcacaagca
     7381 gcagcggccg tcgaagcggc gcgcgcgggc gaagcgggcc gctcgttcgc ggcggtcgcg
     7441 ggcgaggtgc ggatgctcgc gcaacgctac gcggcgtcgt cgaaggaaat caaatcgtgg
     7501 tcgaggcgag tgcaaccgaa gtggcgctcg gcaccgaact ggtggcacgg accacctcgc
     7561 agatgcgcgt gatcgacgaa gcgggtgagc cttttcaccg tcgccgatgc aacacccgtg
     7621 cggcgagctg tgcagccgct gcggcaggca cccacgcgcg acatgacgcc gcatcccgcg
     7681 cacaagccgg cggccggatt cacgcgcgat gctgcacatg gaaccgcgca cgaggtcgag
     7741 ccggatgatt tgacattggt ctgatagatc ggcgttactc ccacctcgac agcccggaat
     7801 tgacgcatca cgatgcacac gtcagcgccc aataagttgc aagggcaggc tgacccgagt
     7861 aacaacggcg tttagcagcc cgtcctattc aaggacgggc tatttttttg ccgagggttt
     7921 cgcgcgccgc gtccggccct aacccaggaa cgcgtcagca ccgtgacagg cggggagaca
     7981 aggattgcac cgtcacgggg cacgaaaaat gcgtccggtg gcgccgggag tcgcgttaaa
     8041 ctgattgtca cagtcgggcg tcacggcccg cattgaccgg cgcggcgcgc cagcgattgg
     8101 ccaacacagc gcagcggcac cgcggcaaac agacaggtga acgggcagac aacgaagtgt
     8161 gagcgcggcg cgacacgcgg cggccaacct gcccgtacaa tggttcgatc aacaacggaa
     8221 gaaggacaac ttcgatgcgc actaccgggt cctccgggtc aatggccctg ctcaccgaat
     8281 acgacgacgc aacggcgcgc gaactccgct cgctgcgcct cgaatcgaca gaagacggca
     8341 aaggcattct tctgatcgag gt
//
LOCUS       DATASH010000833         2692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_337573,
            whole genome shotgun sequence.
ACCESSION   DATASH010000833 DATASH010000000
VERSION     DATASH010000833.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337573"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1223)
                     /locus_tag="VHV99_22380"
     CDS             complement(<1..1223)
                     /locus_tag="VHV99_22380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX3638172.1"
                     /translation="MNKDSAQTALLWIVAAGFFMQSLDTTIVNTALPSIAKSLHVAPL
                     AMQPIVVAYTLTMAMLTPASGWLADRFGTRRVYFTAILVFVLGSICCASAHTLGQLVM
                     ARVLQGFGGSMLLPIGRLAVLRSVSAEQYVSALAFTSIAGQLGPIAGPTLGGWFVQAI
                     TWHWIFLINVPIGAVGLYAVRRFLPEHGETKAPPFDFVGCGLLSLCMIAFSLAIDSPV
                     ETHRAAWSAGLFALAVVSALAYIPHAKRRSVPLFKLSLFSEPNFSVGLIGNLVCRIGS
                     SAVPFLVPLLLQLQLGYSPLHSGLMMLPAAIAGTFAKRWIAPLIRRYGYDTFLLVNTV
                     IVGSSIVAFALITRATPIIVGIVILAVFGAANSMQFAAMNSVTLKGLSHEDAGSGNSL
                     FSMVQMLAIGLGVSIG"
     gene            1386..2246
                     /locus_tag="VHV99_22385"
     CDS             1386..2246
                     /locus_tag="VHV99_22385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3638173.1"
                     /translation="MLNPLWLKTFATVAACHSFTEAGRRLDLTQSSVSEHIRRLEQSV
                     GRRLLVRDTHSLAMTPDGEAMLAHASVILQALARAESQFRAPRLKGRVRLGSSDDVAL
                     GPLPTVLAAFRDAHPDVELEITIGMTGKLYELLDAGSIDLLVGKRRLGDRRGVPLFTG
                     RLEWLARSGTLVDLSQPLPLILVAEPSVTRAVVLDALAEAGFSWQVVCTSSSHAGCIA
                     AARGGLGITVRPQYLAARGLAPPLNAASLPTLPSVEFIALAAKRLSRPAGTLLQLLHD
                     SDLRGSWIGE"
BASE COUNT          541 a          836 c          871 g          444 t
ORIGIN      
        1 ccgatcgaaa cgcccagccc gatcgccagc atctgcacca tcgaaaagag gctgttgccg
       61 ctcccggcat cttcgtgcga gagaccctta agcgtgacgc tgttcatcgc cgcgaactgc
      121 atggagttgg ccgcgccgaa cacggccaga atcacgatcc cgacgataat tggcgtggca
      181 cgcgtaatca atgcgaacgc aacgatcgat gagccgacga tcaccgtgtt gacgagcagg
      241 aacgtgtcat agccatagcg gcgaatgagc ggggcgatcc agcgtttggc gaaagtgccg
      301 gcgatcgccg cgggcagcat catcaagccg gagtgcagcg gtgagtaacc cagttgcagt
      361 tgcagcagca atggcaccag aaacggcacg gcgctcgagc cgatgcggca caccagattg
      421 ccgatcagac cgacgctgaa attcggttcg ctgaagagcg agagtttgaa gagcgggacg
      481 ctacggcgtt ttgcgtgcgg aatataagcg agcgcgctga ccacagcgag cgcgaacaga
      541 cccgccgacc atgccgcacg gtgcgtttcc acgggcgagt cgatcgccag cgagaacgcg
      601 atcatgcaca gcgacagcag cccgcaaccg acgaagtcga atggcggcgc cttcgtttcg
      661 ccatgctccg gcaagaagcg ccgtaccgcg taaaggccca ctgcgccgat cggcacattg
      721 ataagaaaga tccagtgcca cgtaatggcc tgcacgaacc agccgccgag cgtcggcccg
      781 gcgatcggcc ccaactggcc ggcaatcgaa gtgaacgcga gcgccgacac gtattgctcg
      841 gctgagacgc tgcgcagcac ggcaagccgt ccgatcggca gcagcatcga accgccgaag
      901 ccttgcagga cccgcgccat gaccagttgg ccgagcgtat gcgcgctcgc gcagcagatc
      961 gagccgagca cgaacacgag gatcgccgtg aagtagacgc gccgggtgcc gaagcggtcg
     1021 gcgagccagc ccgaagcggg ggtgagcatc gccatggtca gcgtgtaggc gaccacgatc
     1081 ggctgcatcg cgagcggcgc gacgtgcagg ctcttcgcaa tcgacggcaa cgcggtgttg
     1141 acgatcgtcg tgtcgagcga ttgcatgaaa aagccggcgg cgacgatcca gagcagcgcg
     1201 gtctgggcgg aatccttgtt catattcaat agtgcgacgg ggcagcggga cggaagatcg
     1261 acggcctgtg cccaaggtaa ttattgcggg attccgtcat catattgaca tcctcgtcaa
     1321 tcgggaaccc cactagggtt attgactgtt atcgactttg ccgataacca gggtgacaat
     1381 gacgcatgct caatcccctc tggctcaaga ccttcgccac agtcgccgcc tgccacagct
     1441 tcaccgaggc ggggcggcgg ctcgatctga cgcagtcgag cgtcagcgaa catatccgcc
     1501 ggctcgaaca gagcgtcggg cggcgcctgt tggtgcgcga tacgcattcg ctcgcaatga
     1561 ccccggacgg cgaggccatg ctcgcgcacg ccagcgtgat cctgcaagcg ctcgcgcggg
     1621 ccgagtcgca gtttcgcgcc ccgcgcctga aagggcgggt gcggctcggt tcatcggacg
     1681 atgtcgcgct cggtccgctg cccaccgtgc tcgcggcgtt tcgcgacgca catcccgatg
     1741 tcgagctgga aatcaccatc ggcatgaccg gcaagctgta tgagttgctg gatgccggtt
     1801 cgattgatct tctcgtcggc aaacggcgtc tgggcgaccg ccgcggcgta ccgcttttca
     1861 cggggcgcct ggagtggctg gcacgcagcg gcacgctcgt cgatctcagc cagccgctgc
     1921 cgttgattct ggtcgccgaa ccgagcgtga cgcgcgcagt ggtgctcgat gcgctcgccg
     1981 aagccgggtt cagttggcag gtggtgtgca cgagcagcag tcacgcgggt tgtatcgcgg
     2041 ccgcgcgcgg cgggctcggc attacggttc gcccgcaata tctggccgcg cggggactgg
     2101 cgccccctct caatgccgcc agtctgccga cgctgccctc ggtggaattc atcgcgctgg
     2161 ccgccaaacg gctgagccgt ccggcaggca cgttgttgca attgctgcac gacagcgatt
     2221 tgcgcggttc gtggatcggt gagtgaaacc gtgcggcaga cgcgtgggtg agttgagaat
     2281 gcgctgaatg cagccgttca gcgcttagga tcaccaggca catgcacatg cacatgcaca
     2341 tgcacatgca acatcagggc acgtgccttg attcaggacg acggctgcaa cccgagcgtc
     2401 cttaccgctt acccgcgcga aatcagcgta agtcggccac agccgaatgc tggcggcagt
     2461 ttggaaacta cgtcgaggaa actggtaaag cggcaagaat ccacgaggga gcgattcggc
     2521 acgcgcagac cgaaggcagc ggaattgacc ggcgcttgct gccttttccg agcacgtgga
     2581 gtgtccattg agcctgcggg tcgacatcga ccctaggcag accgtccgct tcaggccacg
     2641 cgagttctgc aagtcgcacc gagtggtccc acacggcgtc ctcaatcgcc ag
//
LOCUS       DATASH010000834        11117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5283223,
            whole genome shotgun sequence.
ACCESSION   DATASH010000834 DATASH010000000
VERSION     DATASH010000834.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 11117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11117
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5283223"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..2318)
                     /locus_tag="VHV99_22390"
     CDS             complement(42..2318)
                     /locus_tag="VHV99_22390"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEX3638174.1"
                     /translation="MTYVVAVRAMCEFTARRGDLDLRFTPAPTSLEGMAGHVTVAGRR
                     ASGYETEITLTGTHRGLTVRGRADGYDPAANRLEEVKTHRGALERMPANHGTLHWAQA
                     LVYGHLLCRARGLAELTVALVYFDVGSQKETLLSETHTARELEIFFVEQCERFLDWAV
                     QEAAHRAARNAALGALQFPHGQFRSGQRELAVSVYRAARDGKPLMAQAPTGIGKTLAT
                     IFPLLKACASDQIDRVFFLTAKTPGRALALDAIETLHQGADQSAVRLPLRTLELVARD
                     KACEHPDKACNGESCPLARGFYDRLDAARSAALAGPRLDRASVREAALAHDICPYYLA
                     QELARWSDVVVGDYNYYYDASAMLYALTQINQWRVGVLVDEAHNLLDRARRMYSASLD
                     QAAFRLARKSAPATLKKPLERLQREWNAVKRAQTGTYQVYADVPDKLLSAAQNLIGAV
                     TDQLAEAPLSIDDASLRFFFDTMHFVALAEQFGEHSIFDITLTAERYTPRDKTSATLC
                     VRNVIPAPFLAGRYAAARTTVMFSGTLNPHHFYRDTLGLPEQTRWLDVEGPFRAEQLQ
                     VRVAGHVSTRWRDRERSLVPIVELIAKQYAAQPGNYLGFLSSFDYLQQVVALMRERHP
                     ELPVWAQEPGMDEAARDAFLARFRIMDQGVGFAVLGGAFSEGVDLVGQQLIGAFIATL
                     GLPQVNDVNEQMRRTMEAKFGNGYDYMYLYPGLQKVVQAAGRVIRTEQDQGVVHLIDD
                     RYRRPEVRRLLPRWWQVQ"
     gene            complement(2315..4309)
                     /locus_tag="VHV99_22395"
     CDS             complement(2315..4309)
                     /locus_tag="VHV99_22395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013341252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HEX3638175.1"
                     /translation="MATDAPSDIRSDAPYYLLNFERALAWLAERYDDLLDAHEHGFLR
                     DFAALPQHSRALLVRMLMRKGTLFRASRLSYDEIGCPLQAAAPLTALGWVDAEPALTL
                     DELFTLTTRPELLLIFADAIALIPGAKGLRKPDLLETLRPFYEGEGEGEGEGEGEGEG
                     EGEGEGEGEDKSEDEGEDEGADEGKERGAAAFPLSTWHSQTTDRVFHVAIAPLCERLR
                     LMFFGNLQQDWSEFVLADLGVFQYEKVAFAASSRAFQQRVDVDVYLALHACREALEWL
                     PTGEPEAEAIDELVAVIAAIEIANPWLETRRAKLLFRIGQHCERRQNWAAALAVYERC
                     AWPGARHRRLRVLERSERFDEAFALASQAAATPESEEEAQRVARMLPRLQRKRGERVA
                     RVPAARPVERSTLVLPRPQTPRSVEYVARDHLTCDSAPVHYVENALINSLFGLLCWEP
                     VFAALPGAFFHPFQRGPADLHAADFHARRAGQFAACLAQLDSGAYREAILRHLQSKAG
                     LQSPFVFWGLLTPELVALALDCLPAAHLKLWFERLLRDIRGNRSGLPDLIRFWPAERR
                     YELIEVKGPGDRLQDNQIRWLAYCAEHGMPVRVLDVRWADEDVAVSDVVAKVEIGEAA
                     EVAEVGATAVSNSAAAEAGSAAPNSAAAETRAMVPKEATA"
     gene            complement(4457..6325)
                     /locus_tag="VHV99_22400"
     CDS             complement(4457..6325)
                     /locus_tag="VHV99_22400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S10"
                     /protein_id="HEX3638176.1"
                     /translation="MALRFYLSLRQSLAAGSMAAALVAMALVFAVLAAFSQMCAAAEM
                     DDSSAATGRAETAASPASPVSVPAMPDMPAVADTPAMPAMPAMPDMPGVSDAQTETSG
                     QAVDTNGQPAKQPRANPSRTGRAPQVSATGRNDAQDNPQHNAKPDAAAIPVPPESAAV
                     TQHSIRLDGRKIDYTATAGNLLLHDRTGQANASVFYVAYTVATKAPATRPVTFLFNGG
                     PGAGSVFLMIGSFGPKRARTASPAITPPAPYVLDDNPDSLLGSTDLVFIDAVGTGLSK
                     VVGHGAGKDFWGVDKDLDAFSQFIDRYLTVNQRWNSPKYLLGESYGTARAAMLAYQLG
                     QNNIALNGVILMSSVLDSAAFSPGSDFKSESYLPSFAAIAWYHGKIVPKPASLPAFLD
                     EARAFARGPYAQALALGDALPDAQRDEIAARIARFTGLDVDYVKRSRLRLYPSRFRSQ
                     LLRDQSRSIGRYDARFEGIDYDGVSERPDFDASVSSVSSAFDAALHQHFAQDLHFIPT
                     DRYRVFNDAALTQWDWKHREWWGEHQSVPYAAGDLAEAMRQNPQLRVMSLNGYFDLAT
                     PFYATEYALAHLGLDAPLRANVRITYYPTGHMIYLDDAALHALRRDLASFYAAGGG"
     gene            complement(6480..7691)
                     /locus_tag="VHV99_22405"
     CDS             complement(6480..7691)
                     /locus_tag="VHV99_22405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX3638177.1"
                     /translation="MRYSNLVERLQGRRTSAWEIHRVAQQALARGEDVIVLSVGDPDF
                     ATPAPIVERAVEALRGGDTHYSAVSGREPLRAAIAAEHTSATGCAVNTANVILTAGAQ
                     NGVFATSLCLLQAGDEVIVPEPMYLTYEACVRAAGATLVPVPVDPARAFHLDCDALEA
                     AVTPRTQAIFFETPCNPTGVVMPRADLERIARIACEHDLWVLSDEVYAELTFEREHVS
                     VAALPGMAERTVTLGSLSKSHAMAGWRVGWAIGPATLIEHMGRLALAMHYGLPGFIQQ
                     AALTALQNKAQVVAEMREIYRRRRDVVFERLHRVPGLRCLLPEAGMFMMVDVSGTDLD
                     TVDFTWQLFRAQGVSLLDASAFGETANGFVRLGFVVEEASLIDACERIAAFVGGLRAV
                     KDGAGRRKPGA"
     gene            complement(7837..9318)
                     /locus_tag="VHV99_22410"
     CDS             complement(7837..9318)
                     /locus_tag="VHV99_22410"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013341258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEX3638178.1"
                     /translation="MPSIELRTRQSLALTPRLQQSVRLLQLSSLEFQQELRTALDTNP
                     FLEYDSTDTEDVALATAAPGEDAGAMPATDAVAAAEPESMPSEAGGNDTMEGAGQDDM
                     PGDFSGDYSSRSSTRQNGDSESSDPAEWARSQPTLREQLHDSLRLYRLDDRDRAVARF
                     IIEALDDDGYLRQELSDLADSVDLEPELTEEELLVALRLVQSLDRPGLGARSLSECLS
                     LQVNALPADTPGREVARQIVEHHLERLARREQAELQKQIGCSAEELRVACALVRKLDP
                     KPGNSYGRTEDNYVVPDVIVRQVRNKWVVTINPAVQPRARIHRMYAELFAQSAGASRS
                     PLAQQLQEARWLIRNAQQRFDTIQRVAECIVAHQKAFFQYGEIALKPMVLRDVAEELG
                     LHESTISRATGNKYMATPRGIFEFKHFFPRELGTESGGTCSAAAVRALLKEMIAAENT
                     RDPLSDVTLARMLADQGVLVARRTVAKYRHLMKVPPAELRRQI"
     gene            9848..10180
                     /locus_tag="VHV99_22415"
     CDS             9848..10180
                     /locus_tag="VHV99_22415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013341259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX3638179.1"
                     /translation="MKTIQLLKTAGSIVCVAFALNATAQSSAPSAASETIGQHVDDGT
                     ITTKAKASLLSAKNVKSTHIHVKTRNGVVWLTGSVPSADDKAAAGEVVKGLDGVSSVK
                     NHLKVTAE"
     gene            complement(10583..11035)
                     /locus_tag="VHV99_22420"
     CDS             complement(10583..11035)
                     /locus_tag="VHV99_22420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2169 family four-helix-bundle protein"
                     /protein_id="HEX3638180.1"
                     /translation="MATNVISVLNDLVETSKDGEKGFRKAAEDAHEAQLKSLFLSRAE
                     DCTRGARELQDAVQALGGKPETGGSVSGALHRGWVDVKSAVSNRSDHDILAECEKGED
                     VAKKRYHDALEKELPADVRAIVERQYQGVLQNHDRVRDLRDQYAAAKR"
BASE COUNT         2034 a         3609 c         3480 g         1994 t
ORIGIN      
        1 gcctgttgca cttgcctctt gcacctgcct gctgcacctg cctactgcac ctgccaccag
       61 cgcggcagca accgccgcac ttccggccgc cggtagcggt cgtcgatcag atgcaccacg
      121 ccctggtcct gctccgtacg aatgacgcgg cccgcagcct gcacgacctt ctgcaacccc
      181 ggatacagat acatatagtc gtagccgttg ccgaacttcg cctccatggt gcgtcgcatc
      241 tgctcgttga cgtcgttcac ttgcggcagg ccgagcgtcg cgataaaggc gccgatcaac
      301 tgctggccga ccagatcgac gccctccgag aacgcgccgc ccagcacggc gaagcccaca
      361 ccctggtcca tgatgcgaaa gcgcgcgagg aacgcgtcgc gcgccgcctc gtccatgccc
      421 ggctcctgcg cccacaccgg caattccgga tgacgctcgc gcatcaacgc caccacctgc
      481 tgcagatagt cgaagctgct caggaacccg agataattgc ccggctgcgc cgcgtactgc
      541 ttcgcgatca gctcgacgat cggcacgagc gaacgttccc ggtcacgcca gcgcgtcgag
      601 acatggccgg ccacacgcac ctgcaattgc tcggcgcgaa acggcccttc gacgtcgagc
      661 cagcgggtct gctcgggcaa acccagcgtg tcgcgataga aatgatgcgg attgagcgtg
      721 ccggaaaaca tcaccgtggt gcgcgcggca gcgtagcgcc ccgcgagaaa cggtgccggg
      781 atcacattcc gcacgcacag cgtggcactg gttttgtcgc gcggcgtgta gcgctccgcg
      841 gtgagggtga tgtcgaaaat cgaatgctcg ccgaattgct ccgccagcgc gacgaaatgc
      901 atggtgtcga agaaaaaccg cagcgacgca tcatcgattg atagcggggc ttcggcgagt
      961 tgatcggtca ccgcgccgat cagattctgg gcggccgaaa gcagtttgtc cgggacgtcg
     1021 gcatagacct gatacgtgcc ggtctgtgcg cgcttgacgg cgttccactc acgctgaagc
     1081 cgttcaagcg gcttcttcaa cgtggcgggc gcgcttttgc gtgcgagccg gaacgccgcc
     1141 tgatcgagcg acgcgctgta catgcgccgt gcccggtcga gcaggttatg cgcctcgtcg
     1201 accagcacgc ccacgcgcca ctgattgatc tgcgtcagcg cgtacagcat cgcgctggcg
     1261 tcgtaatagt agttgtaatc gccaaccacc acgtcggacc agcgcgccag ttcctgcgcg
     1321 agatagtacg ggcagatgtc gtgcgcgagg gccgcttcgc gcaccgacgc ccggtcgagc
     1381 ctcggccccg ccaacgccgc gctgcgggcc gcgtcgagcc ggtcatagaa gccgcgcgcg
     1441 agcggacagg actcgccgtt gcaagccttg tcgggatgct cgcaggcttt gtctcgcgcg
     1501 accagttcca gcgttctcaa cggcaagcgc accgcggact ggtccgcgcc ttgatggagg
     1561 gtttcgatcg cgtcgagggc aagcgcgcgg cccggcgtct tggcggtgag gaagaacacg
     1621 cggtcgatct ggtccgatgc gcacgccttg agtagcggaa aaatcgtcgc cagcgtcttg
     1681 ccgatgccgg tcggcgcctg cgccatcaac ggcttgccgt cacgcgccgc gcgatacacc
     1741 gacaccgcca gttcgcgctg accgctacgg aactggccgt gcggaaactg caaggcgccg
     1801 agcgcggcgt tacgcgcggc gcgatgtgcc gcttcctgca cggcccaatc gagaaagcgc
     1861 tcgcattgct cgacgaagaa tatctccagc tcacgggcgg tgtgcgtttc gctcagcagc
     1921 gtttccttct gactgccgac atcgaagtac accagcgcga ccgtcaattc ggcgagccca
     1981 cgggcacggc acagcagatg accgtacacc agcgcctgcg cccaatgcag cgtgccgtga
     2041 ttggcgggca ttcgttcgag cgcgccacga tgggtcttga cctcttccag ccggttcgcc
     2101 gccgggtcgt aaccgtcggc gcggccacgc acggtcaaac cacgatgcgt gccggtcagc
     2161 gtgatctcgg tttcgtagcc actcgcgcgc cgcccggcga ctgtgacgtg gccggccatc
     2221 ccttccagcg aggtgggcgc gggcgtgaag cgcagatcca gatcgccgcg cctcgcggtg
     2281 aattcgcaca ttgcccgcac ggcgaccacg taggtcatgc ggtggcctct ttcggaacca
     2341 tggccctggt ttccgcggcg gcgctgttcg gagccgctga cccggcttcc gcggcggcgc
     2401 tgttcgaaac cgctgtcgca cccacctccg cgacctcggc cgcctcgccg atctcgactt
     2461 tcgccaccac gtcggagacc gcgacgtcct catcggccca ccgcacgtcg agcacgcgca
     2521 ccggcatacc gtgctccgcg cagtaggcaa gccagcggat ctggttgtcc tgcaaacggt
     2581 cgccggggcc ctttacttcg atcaactcat agcggcgctc ggccggccag aaacgaatca
     2641 ggtccggcag gcctgaacgg ttgccacgga tatcgcgcaa caagcgctcg aaccagagtt
     2701 tcagatgcgc ggcgggcagg caatcgagcg ccaatgcgac cagttccggc gtcagcaaac
     2761 cccagaatac aaacggcgat tgcaggcctg ccttgctttg cagatgccgc agaatcgcct
     2821 cgcgataggc cccgctgtcg agttgcgcga gacacgccgc gaattgtccg gcgcgacgcg
     2881 catgaaaatc cgctgcatgc agatcggccg gcccgcgctg gaacggatgg aaaaacgcgc
     2941 cgggcagcgc cgcgaacacc ggctcccagc acagcaaacc gaacagcgaa ttgatcaacg
     3001 cgttttcgac gtaatgaacc ggcgccgagt cgcaagtcaa atgatcgcgg gcgacgtatt
     3061 cgaccgatcg cggcgtctgg ggcctcggca acaccagcgt gctgcgctcg accggccggg
     3121 cggcaggcac acgcgcgacc cgctcgccac gcttgcgctg caaacgaggc agcatgcggg
     3181 cgacgcgctg cgcttcctct tcgctttccg gcgtggcggc ggcttgcgaa gcgagggcga
     3241 aggcttcgtc gaagcgctcg ctgcgctcca gcacccgcaa acgccgatgg cgcgcgccgg
     3301 gccacgcaca acgctcatag acggcaagtg cggcggccca gttctgtcga cgctcgcaat
     3361 gctggccgat ccgaaacagc agcttcgcgc gacgcgtttc gagccagggg ttcgcaattt
     3421 cgatcgcggc gatcaccgcg acgagttcgt cgatcgcctc ggcttccggc tcgcccgtgg
     3481 gcagccattc gagcgcttcg cggcacgcat gcaacgcaag gtacacgtcg acgtccacgc
     3541 gttgctggaa tgcgcgcgac gatgcagcaa aagccacctt ttcgtactgg aacacgccga
     3601 gatcggcaag cacgaactcc gaccagtcct gctgcagatt gccgaagaac atcagacgca
     3661 gacgctcgca aagcggtgcg atcgcgacgt ggaagacgcg atcggtggtt tggctgtgcc
     3721 atgtggacag gggaaacgcg gcggcgcctc gttctttgcc ttcgtccgcg ccttcgtctt
     3781 cgccttcgtc ttcgcttttg tcttcgcctt cgccttcgcc ttcgccttcg ccttcgcctt
     3841 cgccttcgcc ttcgccttcg ccttcgcctt cgccttcgta aaacggtcgc agcgtttcca
     3901 gcagatcggg cttgcgcaag cccttcgcac cggggatcaa cgcgatggcg tccgcaaaga
     3961 tcagcagcaa ctcgggacgc gtggtgagtg tgaacagctc gtcgagcgtc agagccggtt
     4021 cggcgtcgac ccagccgagt gcagtcagcg gcgcagccgc ctgcaagggg caaccgatct
     4081 cgtcgtaaga aagacggctg gcgcggaaca gcgtgccttt gcgcatcagc atgcgaacca
     4141 gcaacgcgcg cgagtgctgc ggcaaagcgg cgaaatcgcg cagaaagcca tgttcgtgcg
     4201 cgtccagcag atcgtcgtaa cgctcggcga gccacgccag cgcacgctcg aagttcagca
     4261 ggtaataagg ggcatcagag cggatgtctg acggagcatc tgtcgccacg gagctttcac
     4321 gaaacctgta tgtttataca gtgatgatat caaaatccgt ggaggggtct gccggtaatg
     4381 cgttgccgaa agtggcaaag cgtcgccaga agtttcaatg ccgaccgcaa cgcacgcacc
     4441 gctgcagaca atgaaactag cccccacccg ccgcataaaa gctcgccaga tcccgcctga
     4501 gcgcatgcaa cgcggcatcg tcgagataga tcatgtgacc ggtcgggtag tacgtgattc
     4561 gcacgttggc gcgcagtggc gcgtcaagcc caagatgcgc gagcgcgtac tcggtcgcat
     4621 agaacggcgt ggccaggtcg aaatagccgt tcaacgacat cacgcgcagt tgcggattct
     4681 gccgcatcgc ttcggcgagg tcgcctgccg catagggcac gctctggtgt tcaccccacc
     4741 actcgcgatg cttccagtcc cactgcgtca acgccgcgtc gttgaagacg cgatagcggt
     4801 cggtggggat gaaatgcagg tcttgcgcga agtgctgatg gagtgcggcg tcgaaagcgc
     4861 tcgacacgct gctcaccgat gcatcgaaat ccggacgctc ggacacgccg tcatagtcga
     4921 tcccttcaaa acgtgcatcg tatcggccga tgctgcgcga ctggtcgcgc agcaattgac
     4981 tgcgaaagcg cgatggatag agacgcagcc ggcttcgctt cacgtaatcg acgtcgagcc
     5041 cggtgaaccg cgcgatgcgc gcggcgatct cgtcgcgctg cgcgtcaggt aatgcgtcgc
     5101 ccagcgcgag cgcttgcgca tagggccccc gtgcgaacgc gcgggcttcg tcgagaaagg
     5161 ccggcagact ggcgggcttc ggcacgatct tgccgtgata ccacgcaatg gccgcgaagc
     5221 tcggcaagta gctttcgctt ttgaaatcgg agcccggtga aaacgccgcg gaatcgagta
     5281 cggacgacat caggatcacg ccgttgagcg cgatattgtt ttgccccagc tgatacgcga
     5341 gcatcgcggc gcgcgcggtg ccgtacgatt caccgagcag atacttcggc gaattccagc
     5401 gctggttcac cgtcagatag cgatcgatga attgcgaaaa cgcgtcgagg tccttgtcga
     5461 cgccccagaa gtccttgccg gcgccatgcc cgacgacctt cgacaggccc gtgccgacgg
     5521 catcgataaa gaccagatcg gtggaaccga gcagactgtc ggggttgtcg tccagcacat
     5581 aaggcgcggg cggcgtgatc gcgggactcg ccgtgcgcgc ccgcttcggc ccgaacgagc
     5641 cgatcatcag gaacacactg ccagcgcccg gtccgccgtt gaagaggaat gtgacggggc
     5701 gcgtcgcagg cgccttggtg gcgaccgtgt aggcgacgta gaaaacgctc gcattggcct
     5761 ggccggttct gtcatgcagc agcaggttgc cggcagtcgc cgtgtaatcg atcttgcggc
     5821 cgtcgagccg gatcgaatgc tgggtcaccg cggcgctttc cggcggcacg gggatcgccg
     5881 ccgcatcggg tttggcgttg tgctgcgggt tgtcttgcgc atcgttccgg ccggtcgcgg
     5941 aaacctgtgg tgcgcggccg gtacgcgatg gattcgcgcg gggctgcttg gcgggttgac
     6001 cgtttgtatc gacggcttgc ccggaagtct ctgtctgagc gtcggacacg ccgggcatat
     6061 cgggcatggc gggcatggcg ggcatggcgg gcgtgtcggc cactgcgggc atgtcgggca
     6121 tggcgggcac actcaccggg ctggccggcg acgccgctgt ttccgcacgg ccagttgcag
     6181 cggatgaatc gtccatttcg gccgccgcgc acatctgact gaacgcggcg aggacggcga
     6241 agacgagcgc catcgccacc agcgcggccg ccatggatcc tgccgccagt gattgacgca
     6301 acgaaagata gaagcgaagg gccatggcgt ttccggctgc gagtcgatat gcgtcggtgt
     6361 tgcgttgcga tgttgcttgg cgctgttgcg tcgatatggc tgtcgaccat atcaagacga
     6421 actcggcgaa cgattcttga cgcaagcatc gcagccgtca atcgaaaact ggccgcgatt
     6481 caggcgcctg gctttcgcct gcctgcaccg tccttcaccg cccgcaatcc gccgacgaac
     6541 gcggcaatcc gttcgcacgc gtcgatcagg ctggcttcct cgaccacgaa ccccagccgc
     6601 acgaaaccat tcgcggtctc gccgaaggcg ctggcgtcga gcagcgacac gccttgggcg
     6661 cgaaacagtt gccaggtgaa atcgaccgtg tcgaggtccg tgccgctgac gtcgaccatc
     6721 atgaacatgc ccgcttcggg cagcaggcaa cgcaagcccg gtacacgatg caagcgctcg
     6781 aacaccacgt cgcggcgacg ccggtatatc tcgcgcatct cggcgacgac ctgcgccttg
     6841 ttctgcaagg cagtcaacgc tgcctgctgg ataaagcccg gcaaaccata gtgcatggcc
     6901 agcgcgagcc gccccatatg ctcgatcagc gtggcaggtc cgatcgccca gcccacccgc
     6961 caccccgcca tggcatgcga cttcgacaga ctgccgagcg tcacggtacg ttccgccatg
     7021 ccgggcagcg cggcaacgct gacgtgctcg cgctcgaagg tgagctccgc atagacctcg
     7081 tcggagagga cccacaagtc gtgttcgcaa gcgatccgcg cgatgcgttc gagatcggcg
     7141 cgcggcatca ccacgccggt cggattacag ggtgtctcga agaagatggc ctgggtgcgc
     7201 ggtgtgacgg ccgcttcgag cgcgtcgcag tcgaggtgaa aagcgcgcgc gggatcgacc
     7261 ggcaccggca cgagcgtggc gcccgcggca cgcacgcagg cctcgtaggt gagatacatc
     7321 ggctccggaa cgatcacttc gtcgcctgct tgcagcaggc acagcgaggt tgcgaacacg
     7381 ccgttctgcg cgcccgccgt caggatcacg ttggcggtgt tcacggcaca accagtggcg
     7441 ctcgtgtgtt cggctgcaat cgccgcacgc agcggttcac gtccggacac cgcgctgtag
     7501 tgcgtatcgc cgccgcgcaa ggcctcgacc gctcgctcga caatcggcgc gggcgtcgcg
     7561 aagtcgggat cgcccacgct cagcacgatc acatcttcgc ctctcgcgag ggcctgttga
     7621 gcgacgcgat gaatttccca cgccgacgta cgcctgcctt gcaaacgctc gaccagattc
     7681 gaatacctca tggaacacgc cccgtttcga cttgagccgc caatgtaatg gggcgtgcgc
     7741 gaaacgtcca gatacgaaaa agcgccacgc gagcacggag tctcgcgtgg cgcattagcg
     7801 atggccctcg tgtgagagtc cgcaacaagg ctgcccttaa atctggcggc gcagttccgc
     7861 cggcggcacc ttcatcaggt gacggtattt cgccaccgtg cgccgcgcca ccagcacgcc
     7921 ctgatcggcg agcatcctgg cgagcgtcac atccgacagc gggtcgcgcg tattctccgc
     7981 tgcgatcatt tccttgagca gcgcgcgcac ggccgcggcc gagcaggtgc cgccgctttc
     8041 cgtgccgagc tcacgcggga agaagtgttt gaactcgaaa atgccgcgcg gcgtagccat
     8101 atatttgttg ccggtcgcac gcgagatggt ggactcatgc aggccgagtt cttcggccac
     8161 atcgcgcagc accatcggct tgagcgcgat ttcgccgtac tggaagaacg ccttctgatg
     8221 cgcgacgatg cactcggcca cgcgttgaat cgtatcgaag cgctgctgcg cattgcggat
     8281 cagccagcgc gcttcctgca actgctgcgc aagcggcgag cggcttgcac cagccgactg
     8341 cgcgaacagt tcggcataca tgcgatggat gcgagcgcgc ggctgcacgg ccggattgat
     8401 cgtcaccacc cacttgttgc gcacctgacg cacgatgacg tccggcacca cgtagttgtc
     8461 ttcggtacgg ccgtagctgt tgcccggctt cgggtcgagt ttgcgcacca gcgcgcaggc
     8521 cacccgcagt tcctcggcgc tgcagccgat ctgcttttgc agctcagcct gttcgcggcg
     8581 cgcgagacgt tcaagatgat gttcgacgat ctgccgcgcc acctcgcggc ccggcgtgtc
     8641 ggcgggcaac gcgttgactt gcagcgatag acactcggac agcgagcggg cgccgagacc
     8701 cgggcgatcc agcgactgca cgaggcgcag cgcaaccagc aattcttctt cggtcagttc
     8761 aggttcgagg tcgacgctgt ccgcgagatc ggaaagctcc tgacgcagat agccgtcgtc
     8821 gtcgagcgcc tcgatgatga aacgggccac cgcgcggtcc cggtcatcga gccggtacag
     8881 ccgcagcgag tcgtgcaact gctcgcgcaa ggtgggctgg gaacgcgccc attcggcggg
     8941 atcgctactt tcggaatcgc cgttctggcg cgttgaactg cggctgctgt agtcgcccga
     9001 aaaatcaccg ggcatgtcat cctgccccgc gccttccatc gtgtcgttgc cgccggcttc
     9061 agacggcatg gattcaggtt cggccgcggc gaccgcgtca gtggcgggca tcgcgcccgc
     9121 gtcttcgccg ggcgccgcgg tggcgagggc gacatcttca gtgtccgtcg agtcgtactc
     9181 gaggaacgga ttggtgtcga gtgcggtgcg caactcctgc tggaactcca gagacgacaa
     9241 ctgcaacaga cgcaccgatt gctgcagacg cggcgtgagt gcgagagact gccttgtgcg
     9301 tagttcaatt gaaggcattg tagtgaggtc ccttaatagc tgagaaatgg tgtgcgagtg
     9361 tttggcctct acagagcaac tgtcatacca gctcgtccaa cacactgttt tttctgcgtt
     9421 ttgccttttc aggcgcgcgg attgcgcagc aggtgggcgc catttttaca aggccccggc
     9481 aatttccggc cctgggcctt gcgggccgcc gtatcgcgcg ccgcacctgc ccgctgccct
     9541 attcctcagc ctaaacgcat gtgccgtaac ggcttttccc atcatcgggt aagcccgccg
     9601 gttgccgggc acgcaccttg cgcttcagca ctatgtggac ggacgttggt ctttgcacgc
     9661 gagtcgatcg cccggtaacg cggtggcgaa agacagggag cgcatgcaca ggccgttgtc
     9721 cggtgtcgat aacgggccct gacggaaccg tagcggcaac gaccccacgc gagtcatcaa
     9781 cggtttgaac cgcgatcgac cgcaggcaac aagaccgcat ttttcatcaa tacccgaagg
     9841 agaaaccatg aagaccatcc aacttttgaa gaccgcaggt agcatcgttt gcgtcgcatt
     9901 cgcactgaac gctacggcgc aatccagcgc cccgtcggca gcgtccgaaa ccatcggcca
     9961 gcacgtcgac gacggcacca tcaccaccaa ggccaaggct tcgctgctga gcgcgaagaa
    10021 cgtcaagtcg acccatatcc acgtgaagac ccgcaacggc gtggtgtggc tcaccggttc
    10081 agtgccgtcg gccgacgaca aggctgccgc tggagaagtc gtcaagggcc tggacggtgt
    10141 ctcgagcgtg aagaaccatc tgaaggtcac tgcggaataa gatcgggcga tccgatagaa
    10201 aggagtcggc ttcggccggc tctttttttg gccgggcgtt tctggtgaga cagagagcca
    10261 ccgtactttg gtgagacaga cagacgtttt tggcgggact aacagccacc actttttggc
    10321 gggatagaca gtcgacgttt ttggcgagac agtcgtcagg atagcgtcgt ggcaagaata
    10381 gaaaagccgt ccgcaattca aatgtggacg gctttttcgt tgcagggcgg cttatgtcgc
    10441 ggcgttatct ttgtactggg ccttagggtc gatgccctat tgcatgacag tttcaactgc
    10501 ccctgttccc atggtctgaa acaaaccggc gctcgttaaa gcgccggcct gcggctcact
    10561 ccgccgctac cgccgctgcc aattaacgct tcgctgcggc gtactgatcg cgcagatcgc
    10621 gcacccggtc gtgattttgc agcacgcctt gatattgacg ctcgacgatg gcgcgcacgt
    10681 ccgcaggcaa ttccttttcg agcgcgtcgt gataacgctt cttcgcgacg tcttcgccct
    10741 tttcgcattc agcgaggatg tcgtgatcgc tgcgattcga gaccgccgac ttcacatcga
    10801 cccacccgcg atgcaatgcg ccgctgacgc tgccgcccgt ttcgggcttg ccgcccaaag
    10861 cctgcacggc atcctgcaat tcgcgcgcgc cgcgcgtgca atcttcggca cgggagagga
    10921 acagcgactt caattgtgcc tcatgcgcgt cttctgctgc tttgcgaaag cccttctcgc
    10981 catccttcga cgtttcgacc agatcgttca gcacggaaat aacgttcgta gccattcact
    11041 acctccaggg gtttcgttga tcgtggaatc cgctgccggc ctctgcgtac ttgtgcgaaa
    11101 caggcaatcg catcggc
//
LOCUS       DATASH010000835         2604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3700973,
            whole genome shotgun sequence.
ACCESSION   DATASH010000835 DATASH010000000
VERSION     DATASH010000835.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3700973"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VHV99_22425"
     CDS             <1..281
                     /locus_tag="VHV99_22425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638181.1"
                     /translation="APTYETYVHDFIPFEWRLPRIFARSDRAGKMSGHDGRSAFRDRF
                     RGNRFDERFDGNGELNHTGRFAVQFGNRGDLGDLRHRYNIHENRNLAT"
     gene            complement(411..1478)
                     /gene="leuB"
                     /locus_tag="VHV99_22430"
     CDS             complement(411..1478)
                     /gene="leuB"
                     /locus_tag="VHV99_22430"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014725192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEX3638182.1"
                     /translation="MKIAVLPGDGIGPEIVKEAVKVLNVLGEKFELEEAPVGGAGYET
                     KGHPLPDSTLALAKEADAILFGAVGDWKYDSLERALRPEQAILGLRKHLQLFANFRPA
                     ICYPQLTGASSLKEEIVSGLDILIVRELNGDIYFGSPRGVREAPDGLFAGSKEGFDTM
                     RYSEPEVRRIAHVAFQAAQKRSKKLTSVDKANVLETSQLWKDVMIDVSKEYADVELSH
                     MYVDNAAMQLVKAPKSFDVIVTGNLFGDILSDEAAMLTGSIGMLPSASLDKNNRGLYE
                     PSHGSAPDIAGKGVANPLATILSAAMMLRYSLNKAEQADRIENAVKKVLEQGYRTGDI
                     LTPGCKQVGTVAMGDAVVAAL"
     gene            complement(1505..2158)
                     /gene="leuD"
                     /locus_tag="VHV99_22435"
     CDS             complement(1505..2158)
                     /gene="leuD"
                     /locus_tag="VHV99_22435"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010099619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX3638183.1"
                     /translation="MDKFIVHTGVVAPLDRENVDTDAIIPKQFLKSIKRTGFGPNAFD
                     EWRYLDHGEPGQDNSKRPLNPDFVLNQPRYQGASVLLARKNFGCGSSREHAPWALGQY
                     GFRAIIAPSFADIFYNNCFKNGLLPIVLTEQQVDHLFNETYAFNGFKLTVDLEAQVVR
                     TSDGGTEYPFEVAAFRKYCLLNGFDDIGLTLRHADKIRQFEAERITKQPWLAHRIVG"
     gene            complement(2245..2403)
                     /locus_tag="VHV99_22440"
     CDS             complement(2245..2403)
                     /locus_tag="VHV99_22440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HEX3638184.1"
                     /translation="MIKNINRTTLLRRFALGTLTWLLLGLAGCNTVHGFGEDMSHLGN
                     SISNHADK"
     gene            complement(2409..>2604)
                     /locus_tag="VHV99_22445"
     CDS             complement(2409..>2604)
                     /locus_tag="VHV99_22445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013690586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEX3638185.1"
                     /translation="REPGCSMCLAMNADRLDPGERCASTSNRNFEGRQGAGGRTHLVS
                     PAMAAAAAIEGHFVDIRKLG"
BASE COUNT          513 a          796 c          745 g          550 t
ORIGIN      
        1 ccgcgccaac ctacgagacc tacgttcatg acttcatacc cttcgaatgg agacttcccc
       61 gcatctttgc ccgcagtgac cgcgcgggga agatgagcgg gcacgacgga cgatcagcgt
      121 ttcgtgatcg ttttcggggt aatcgttttg acgagcgatt tgatggtaat ggcgagctca
      181 accacacggg ccgttttgcc gtgcagtttg gaaatcgagg agatttggga gatctgcgcc
      241 atcgctacaa tatacacgaa aaccggaatc tggcgacgtg atcgtctgcc gttatcgtcc
      301 gtattacgca cgaattggcc tgtttcaagg ccaattcgcg ttttaaaggc cgctttaaag
      361 actgctcggc gacccaacta ccctctgtgc tacgttcgcg tcaaagcgcc ttacagagcg
      421 gcgacaaccg cgtcgcccat cgccacggtg ccgacctgct tgcaacccgg cgtcagaatg
      481 tcgcccgtgc gatagccctg ttccagcacc ttctttactg cgttttcgat gcgatccgcc
      541 tgttccgcct tgttcagcga atagcgcagc atcatggcgg ccgacaggat cgtggccagc
      601 ggattcgcca cgcccttgcc ggcgatgtcc ggcgcggaac cgtgcgacgg ctcgtacaag
      661 cccctgttgt tcttgtcgag cgaggccgac ggcaacatgc cgatcgaacc agtcagcatg
      721 gcggcttcgt cggagagaat gtcgccgaac aggttgccgg tgacgatcac gtcgaatgac
      781 tttggcgcct taaccagttg catggccgcg ttgtcgacat acatgtgcga caactcgacg
      841 tccgcgtatt ccttcgacac gtcgatcatc acgtccttcc agagctgcga cgtttcgagc
      901 acgttcgcct tatcgaccga ggtcagcttc ttgctgcgct tttgcgccgc ctgaaacgcc
      961 acgtgcgcaa tgcggcgcac ttcgggttcc gaataacgca tcgtgtcgaa gccttctttc
     1021 gagccggcga acagaccgtc cggcgcttca cgcacgccgc gcggcgagcc aaagtaaatg
     1081 tcgccgttca gttcgcgcac gatcaggatg tccagacccg aaacgatttc ttccttcagc
     1141 gacgaagcgc ccgtcagttg cggatagcag attgccggac ggaaattcgc gaacagttgc
     1201 aggtgcttgc gcaaaccgag aatggcctgc tccgggcgca gcgcgcgttc gagcgagtcg
     1261 tacttccagt cgccgacggc gccgaacagg attgcatcgg cttccttcgc cagcgcgagc
     1321 gtcgaatcgg gcagcggatg gcccttcgtt tcgtagcccg cgccgccaac cggcgcttct
     1381 tcgagttcga acttttcgcc gagcacgttc aggaccttga cggcctcctt gacgatttcg
     1441 ggaccgatgc cgtcgcccgg caagactgcg atcttcatgc gaatttcctt gatgattttt
     1501 tcgtttatcc gacgatccgg tgagccagcc aaggctgctt cgtaatccgc tcggcttcga
     1561 actggcgaat cttgtccgcg tgacgcagcg tcaaaccgat atcgtcgaag ccattcagca
     1621 ggcagtactt gcggaacgca gccacttcga acggatactc ggtgccgccg tccgacgtgc
     1681 gcacgacttg cgcttccagg tcgacggtca gcttgaagcc gttgaacgcg taggtttcgt
     1741 tgaacaggtg atcgacttgc tgttcggtca gcacgatcgg cagcaggccg ttcttgaagc
     1801 agttgttgta gaaaatatcg gcgaagctcg gcgcaatgat cgcgcgaaag ccgtattgcc
     1861 ccagtgccca cggcgcatgc tcgcgcgagc tgccgcaacc gaagttcttg cgagccaaca
     1921 gcaccgatgc gccctgatag cgcggctggt tcagcacgaa atccgggttc agcggacgct
     1981 tcgagttgtc ctgacccggc tcgccgtggt cgagataacg ccattcgtcg aacgcgttcg
     2041 gaccgaaacc cgtgcgcttg atcgacttca ggaattgctt cggaatgatc gcgtccgtgt
     2101 cgacgttctc gcgatcgagc ggcgccacga cgccggtgtg tacgatgaat ttatccatga
     2161 cgcttgcgcc tgtttcaaga ccggacgatg cgcgtgacag cctgaaggcc accacccgca
     2221 ctgccggcaa aaatcaaaaa ccgcttattt gtcagcgtga ttgctgatcg aattgccgag
     2281 gtgcgacatg tcctcgccga atccatgcac cgtgttgcag ccggccagac cgagcaatag
     2341 ccaggtcagc gtaccgagtg cgaagcggcg caatagagtg gtgcgattga tgttctttat
     2401 catgcgattt atccaagctt gcgaatatcg acgaaatgcc cttcgatagc cgcagcggca
     2461 gccatcgcgg ggctcaccag atgagtacgg ccaccggcgc cctgacgacc ttcgaaatta
     2521 cggttcgacg tggacgcgca acgctcgccc ggatccaacc ggtcggcgtt catcgcgaga
     2581 cacatcgagc agcccggctc gcgc
//
LOCUS       DATASH010000836         4073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3899691,
            whole genome shotgun sequence.
ACCESSION   DATASH010000836 DATASH010000000
VERSION     DATASH010000836.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3899691"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="VHV99_22450"
     CDS             <1..777
                     /locus_tag="VHV99_22450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX3638186.1"
                     /translation="FISNGVWDFDAFGQSNWSSASLVRGRNWPQSSNNFAYQSPKIAG
                     FDFYGQYALSNATNWNGNGTTPQGREAGAQITYTSSLFQIRGMYDEIRNPANGLLWGG
                     NVGANSNAANTANGVFAASREYTAMFNVFLGQFKIQGAYQAVRSAGATGVLPGQPTTL
                     DHEWGGVTWQATPAAALIAAVYHVNGNNGAGNATIYTFGGSYNLSKRTLLDLQAATVR
                     NSSTANFGLNANNAGFATATDNPLQGHQQSGVYAGIQHSF"
     gene            complement(1059..1397)
                     /gene="glnK"
                     /locus_tag="VHV99_22455"
     CDS             complement(1059..1397)
                     /gene="glnK"
                     /locus_tag="VHV99_22455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX3638187.1"
                     /translation="MKRITAVIKPFKLDEVREALAEVGLTGLTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIEVVVADNQCDQVIDAIIGAARTGKIGDGKIFVADVERVIRI
                     RTGEENEAAV"
     gene            complement(1542..3269)
                     /locus_tag="VHV99_22460"
     CDS             complement(1542..3269)
                     /locus_tag="VHV99_22460"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018431632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEX3638188.1"
                     /translation="MKTRIALAQINVTVGDFAGNVAKIVAAARAAHNDGAKLLIAPEL
                     ALSGYPPEDLLLRPAFYTASAAALTDLATQLKPFAGLHVIVGHPLREVTHPATHVAAH
                     GHGNANAPIERGVPPVDTFNAASLIVDGEVAGTYRKQDLPNTEVFDEKRYFASDPKPF
                     VFELDGVKYGVMICEDAWHASAAQMAKAAGAQVLLIPNGSPYHLNKEAVRFDILRARI
                     RETGLPMVYVNMVGAQDELVFDGGSFVLDGEGQLVAKMAQFEEGHAIVEFDGGKPLPG
                     KPLPGAIAPELSLEAQVYAALVMGVRDYINKNGFPGALIGLSGGVDSALVLAVAVDAL
                     GADRVRAVMMPSRYTADISTTDAAEMARRVGVRYDEIAIAPMFDAFRSSLADEFAGRA
                     EDATEENIQARIRGTLLMALSNKFGSIVLTTGNKSEMAVGYCTLYGDMAGGFAVIKDI
                     AKTLVYKLCHYRNQATAFGKQDVIPERILTRAPSAELRENQTDQDSLPPYEVLDAIMR
                     MYMEEDRSLAEIIAAGYAVDDVKRVTRLIKINEYKRRQAPIGIRVTHRAFGRDWRYPI
                     TSRYTEPVQ"
     gene            3402..>4073
                     /locus_tag="VHV99_22465"
     CDS             3402..>4073
                     /locus_tag="VHV99_22465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidogalycan biosysnthesis protein"
                     /protein_id="HEX3638189.1"
                     /translation="MNQERFDYRTGILASPSEVDAAEWNALLAQQSQPTPFLRHEFLS
                     ALSATRCAVADTGWAPQFVTLTDPRTDKLAAAAPVYLKGHSYGEYVFDWAWADAYKRN
                     GLPYYPKLLCAVPFTPVQGNRLLSADTHALRHLAATLMAFAEQADVSSLHVLFPTEAE
                     GAALTDMGMMQREGVQFHWLNDGYRDFDDFLSTLEQKKRKNIRAERRKVQEAGVTMRR
                     IRGEDI"
BASE COUNT          752 a         1266 c         1260 g          795 t
ORIGIN      
        1 tttatctcga acggcgtgtg ggacttcgac gcgttcggtc aatcgaactg gtcgtcggcg
       61 tcgctggtgc gtggccgcaa ctggccgcaa tcgagcaaca acttcgcgta ccagtcgccg
      121 aagatcgcgg gctttgactt ctatggtcaa tacgcgctgt cgaacgcaac gaactggaac
      181 ggcaacggca cgacccctca aggccgtgaa gctggcgcac aaatcaccta cacgagttcg
      241 ctgttccaga ttcgcggtat gtacgacgaa atccgcaatc cggcaaacgg cttgctgtgg
      301 ggtggcaatg tcggcgcgaa cagcaacgca gctaacacgg ccaacggcgt attcgcagca
      361 tcgcgcgaat acacggccat gttcaatgtg ttcctcggcc agttcaagat tcagggcgcc
      421 taccaggctg tccgttcggc aggtgctacc ggtgttctgc cgggccagcc gaccacgctc
      481 gatcacgaat ggggcggtgt gacgtggcaa gcaacgccgg ctgcggcact gattgcggcg
      541 gtgtaccacg tgaacggcaa caacggtgca ggcaatgcga cgatctacac gttcggcggc
      601 tcgtacaacc tgtccaagcg cacgctgctg gacctgcagg ctgcaacggt gcgtaacagc
      661 agtacggcta acttcggtct gaatgctaac aacgctggtt tcgccactgc aacggataac
      721 ccgctgcaag gccaccagca gtccggcgta tacgcaggta ttcaacactc gttctaagcg
      781 aacagtgtgc cccgagggga cttccttcgg ggcgcccccc aaggggactt ccttcggggc
      841 gtagtcacaa gtttcttctt caagagaatc tttttcacgc tgctgcgaga ggcgcgtatc
      901 ggtgcgatgt ccgaaagggt atcgcaccgt tttttattgg tgcgcacggc tttgccactg
      961 ctccggcgca cctgatcggt gcgcgcgttg cgcgaggcca ataccagcgc tttagcttta
     1021 gtgttttatc gtgcgggttg aatccgcgca cgaaccgctc aaaccgcggc ttcgttttcc
     1081 tcgcccgtgc gaatccggat cacacgctcg acgtccgcga cgaaaatctt gccatcgccg
     1141 atcttgccgg tgcgcgccgc gccgatgatc gcgtcgatca cctgatcgca ctggttgtcc
     1201 gcgaccacca cttcgatctt cactttcggc agaaagtcga ccacatactc tgcaccacga
     1261 tagagctcgg tgtggccttt ttgccggccg aagcctttga cttcggtcac ggtcagaccc
     1321 gtgaggccga cttcagcgag cgcctcgcgc acttcgtcga gtttgaacgg tttgatgact
     1381 gcagtgatgc gcttcatggc ggacctcgtc tcgtgattag aagttcgaaa ggtgaatgga
     1441 tgatttttat tctacgccgc gccggatgcg ttgcggcggt ccggcggtcc ggcgcgcgct
     1501 tcaggtctgc ttcacgtgag tccgcaatgg ccgttcgctt cttactgaac aggttcggtg
     1561 tagcgcgacg taatcgggta acgccagtcg cggccgaacg cgcgatgcgt gacacgaatg
     1621 ccgatcggcg cctgacggcg tttgtactcg ttgattttga tgaggcgcgt gacacgtttc
     1681 acatcgtcga cagcgtaacc cgccgcgatg atttccgcga gcgagcggtc ctcttccatg
     1741 tacatgcgca tgattgcatc gagcacttcg tacggcggca agctgtcctg atcggtctgg
     1801 ttctcgcgca attccgccga gggcgcgcgc gtcagaatcc gctcggggat cacgtcctgc
     1861 ttgccgaagg cggtggcctg gttgcggtaa tggcatagct tgtagaccag cgtcttggcg
     1921 atgtccttga ttaccgcgaa gccgccggcc atgtcgccat ataacgtgca gtaacccacc
     1981 gccatttcgc tcttgttgcc ggtggtcagc acgatcgaac cgaacttgtt cgacaacgcc
     2041 atcagcaagg tgccgcggat gcgcgcctga atgttttctt cggtggcgtc ttcggcgcgg
     2101 cccgcgaatt catcggcgag cgagctgcgg aacgcatcga acatcggcgc gatcgcgatt
     2161 tcgtcgtaac ggacgccgac gcggcgggcc atttcagcgg cgtcggtggt cgagatgtcc
     2221 gcggtgtagc gcgacggcat catcaccgcg cgcacgcggt cggcgccgag tgcatcgacc
     2281 gcgacggcca gcaccagcgc ggaatccacg ccgcccgaca ggccgatcag cgcgcccggg
     2341 aaaccgttct tgttgatgta gtcgcgcacg cccatgacga gtgcggcata cacctgcgcc
     2401 tccagggaga gctccggcgc gatcgcgccg ggcagcggtt tgccgggcag cggtttgccg
     2461 ccgtcgaact cgacgatcgc atggccctct tcgaactgcg ccatcttcgc gacgagctga
     2521 ccttcaccgt cgagcacgaa cgagccaccg tcgaatacca gttcgtcctg cgcgccgacc
     2581 atgttcacgt acaccatcgg caaaccggtt tcgcgaatcc gcgcgcgcag aatgtcgaag
     2641 cgcaccgctt ccttgttcag gtgatacggc gagccgttcg gaatcagcag cacttgcgcg
     2701 cctgctgcct tcgccatttg cgcagccgac gcatgccacg cgtcctcgca aatcatcacg
     2761 ccgtatttca ccccatccag ttcgaacacg aacggcttcg ggtcggacgc gaagtagcgc
     2821 ttctcgtcga acacctcggt attgggcaaa tcctgcttgc gataggtgcc agccacttcg
     2881 ccgtccacga tcagcgaggc cgcgttgaag gtatcgaccg gcgggacgcc gcgctcgatc
     2941 ggcgcgtttg cattaccatg gccgtgcgcc gcgacgtgcg tcgccgggtg ggtcacctcg
     3001 cgcagcggat ggccgacgat cacatgcaag ccggcgaacg gcttgagttg cgtggcgagg
     3061 tcggtcagcg cggccgcgct cgcggtgtaa aacgcgggac gcagcagcag atcttcgggc
     3121 ggatagccgg agagcgcgag ttcaggcgcg atcagcagct tcgcaccatc gttgtgcgcg
     3181 gcgcgcgcgg cggcgacaat cttcgcgacg ttgccggcga agtcgccgac ggtgacattg
     3241 atttgagcaa gagcgattcg ggtcttcatg gcaggatcga caggcaagcc ggatggcttg
     3301 cgagtaccgc ggctggttag ctcgacggca acggacaaat cggataaacg aacgacgcat
     3361 cgcttcacgc ctgcgtcgct cattgggaac agtcacacag attgaaccag gaacgctttg
     3421 attatcgcac gggcattctg gcgtcgccct cggaggtcga cgccgccgaa tggaatgccc
     3481 tcctcgcgca gcagtcgcaa cccacgccgt ttctgcggca cgagtttctg agcgcgctct
     3541 ccgcgacccg gtgcgcggtc gccgataccg gctgggcgcc gcaattcgtc acgctcaccg
     3601 acccgcgcac ggacaagctc gcggcggccg cgcccgtgta tctgaagggg cactcgtacg
     3661 gcgaatacgt cttcgattgg gcatgggccg atgcgtacaa gcgcaacggt ctgccgtatt
     3721 acccgaagct gctgtgcgcg gtgccgttta caccggtgca aggcaaccgg ctgttgtcgg
     3781 cggatacgca tgcgctcaga catctggcgg caacgctgat ggcgttcgcc gagcaagccg
     3841 acgtgtcgtc gctgcacgtg ctgtttccga ccgaagcgga aggcgctgcg ctgaccgaca
     3901 tgggcatgat gcagcgcgaa ggcgtgcagt tccactggct gaacgacggc tatcgcgact
     3961 tcgacgattt cctctccacg ctcgaacaga agaagcgcaa gaacatccgc gccgagcgtc
     4021 gcaaggtcca ggaagccggc gtgacgatgc ggcggattcg cggcgaagat atc
//
LOCUS       DATASH010000837         3861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2909971,
            whole genome shotgun sequence.
ACCESSION   DATASH010000837 DATASH010000000
VERSION     DATASH010000837.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2909971"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1680)
                     /locus_tag="VHV99_22470"
     CDS             complement(<1..1680)
                     /locus_tag="VHV99_22470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011546893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX3638190.1"
                     /translation="MQRSIATVSISGTLVEKLTAIQAAGFEGVEIFENDLLYFDGSPA
                     DVRRIAEDLGLKIMLFQPFRDFDGVSPERLERNLDRARRKFDVMHELGTDRILVCSNV
                     SPDTIGDDSLMTDQLGALARAAEAAGVIAGYEALAWGKHVKTYRHAWKLVNAVNHPNL
                     GLVLDSFHTLSLDDTVDGIAGIPGERIAFVQIADAPKLAMDVLEWSRHYRCFPGQGDF
                     DLAGFTAQVVKTGYSGPLSLEIFNDGFRAAPTTITAADGHRSLLFLEEQTRALLDATQ
                     QPVGDLYQSPAAPAHVGYQFLEFAVDHSTRAQLVDWLGKLRFRQAGQHRSKDVTLFQH
                     GAASIVLNAEPDSFANAFFQQHGLSLCASAFRVDDARQAFERAAGFGYAPFSGQIGPN
                     ERVLPAVQAPDGSLNYFVDETPDQPTLFEADFVLTDVNGPTEVGPLSRIDHVCLSVPA
                     PSLDTWVLFLRTAFGFQAEPGVLVPDPYGLVRSRALRSRDGSVRIILNASVDHHTAVA
                     EALHTYHGSGLNHVAFSTGDIFSAIPEFVADGLPVLRIPRNYYEDLAARYAL"
     gene            2005..2460
                     /gene="aroQ"
                     /locus_tag="VHV99_22475"
     CDS             2005..2460
                     /gene="aroQ"
                     /locus_tag="VHV99_22475"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013342752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEX3638191.1"
                     /translation="MSFASVLVLNGPNLNLLGTREPAFYGSETLDDVAKLCRDAGERL
                     DLSVDFCQSNAEHQLIDWLHAARTKVDGIVINPAAYTHTSVAIADALTAIEKPVIEVH
                     ISNVHRREAFRHHSYVSAVAEAIIIGCGTQGYVLALERMATILKNRAAK"
     gene            2457..3458
                     /locus_tag="VHV99_22480"
     CDS             2457..3458
                     /locus_tag="VHV99_22480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX3638192.1"
                     /translation="MNAQEHTQTSTQTSTQANTQANAQASTQASTRSWLVGLIGSGIG
                     GSLSPAMHEEEGSKLGLHYVYRRIDLEALKLDTTALADLLVAAERMGYNGLNITYPCK
                     QAVIPLLDELSDDARALGAVNTVLFKGGKRIGHNTDWSGFARAFQRGLPDVSLERVVQ
                     LGAGGAGAAVAHAALTMGTQTLTLFDVDAARAASLAAELQKRFPAATVSAGSSLAKSL
                     NESLNESLNESLAAANGLIHATPTGMAKLPGLPLPVELLHRALWVADIVYFPIRTALL
                     QAAEALGCRTLSGGGMAVYQAVDAMRIFTGLEPDAERVYTHFQSLLQRPEESPAAGR"
     gene            3520..>3861
                     /locus_tag="VHV99_22485"
     CDS             3520..>3861
                     /locus_tag="VHV99_22485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3638193.1"
                     /translation="MQQPIQSTLADAAGQTNATVSTARRSKARYQILSLLAVGTMINY
                     LDRTVLGIAAPQLTRELGINAALMGLLFSVFSWSYVVAQIPGGLFLDRFGSKLTYFLS
                     MTFWSLCTLAQG"
BASE COUNT          755 a         1224 c         1208 g          674 t
ORIGIN      
        1 cagcgcgtag cgggcggcca ggtcctcgta ataattacgc gggatacgca acacgggcag
       61 accgtcggcg acgaattcgg gaatcgcgct gaaaatgtcg ccggtgctga acgcgacgtg
      121 attcaagccc gagccgtggt acgtgtgcaa tgcctcggcc accgccgtgt gatgatccac
      181 cgacgcattc aatatgatgc gaaccgatcc atcccggctg cgcagcgcgc ggctgcgcac
      241 cagcccgtac gggtcgggca ccagcacgcc tggctcggcc tgaaaaccaa atgccgtgcg
      301 cagaaacaga acccacgtat ccagcgaagg ggccggcacc gacaggcaga cgtgatcgat
      361 acgcgaaagc ggcccaactt cagtcggacc gttgacgtcg gtcaggacga aatcggcttc
      421 gaacaacgtg ggctggtccg gcgtttcgtc gacgaaatag ttcaggctgc cgtcgggcgc
      481 ctgcacggcg ggcagcacgc gttcgttcgg gccgatctgg ccggaaaacg gcgcgtagcc
      541 gaacccggcg gcgcgctcga acgcctgcct ggcgtcgtcc acgcgaaacg ccgacgcgca
      601 cagcgacaaa ccatgctgct ggaagaaggc attggcgaag gaatccggct cggcgttcag
      661 cacgatcgac gccgcgccgt gctgaaacag cgtcacatct ttcgaacgat gctggccggc
      721 ctggcgaaag cgcagcttgc cgagccagtc gaccagttgc gcgcgggtgc tgtggtcgac
      781 cgcgaactcg agaaactggt agccgacgtg cgcgggcgcg gcgggcgatt gatagagatc
      841 gccgaccggc tgctgcgtgg cgtcaagcag cgcgcgagtc tgttcctcga ggaacagcaa
      901 cgagcgatgg ccgtccgccg cggtgatggt ggttggtgcg gcgcggaaac cgtcgttgaa
      961 aatttcgagc gagagcggcc cgctgtagcc ggttttcacg acctgcgcgg tgaaaccggc
     1021 cagatcgaaa tcgccctggc ccgggaaaca gcggtaatga cggctccatt cgagcacgtc
     1081 catggcgagc ttcggcgcat cggcgatttg cacgaaggcg atgcgctcgc ctggaatccc
     1141 ggcgatgccg tccaccgtat cgtcgagcga cagcgtatga aaactgtcga gcacgagccc
     1201 gagattcgga tgattgaccg cgttgacgag cttccacgca tgccggtagg tcttcacgtg
     1261 cttgccccac gcaagcgctt cataaccggc gatcacgccg gccgcttcgg ccgcgcgggc
     1321 cagtgcgccc aactggtcgg tcatcagcga atcgtcgccg atggtgtcgg gcgagacgtt
     1381 gctgcacacc agaatccggt ccgtgcccag ttcgtgcatc acgtcgaact tgcgcctggc
     1441 ccgatccaga ttgcgctcga gacgctccgg actcactccg tcgaaatcgc ggaacggctg
     1501 gaacagcatg atcttcagcc cgagatcctc ggcgatgcgc cgcacgtcgg cgggcgaacc
     1561 atcgaaatac agcaggtcgt tctcgaagat ttcgacgcct tcgaagcccg ccgcctggat
     1621 ggcggtcagc ttctcgacga gggtcccgct gatcgacacg gtggcaatcg aacgttgcat
     1681 gaacggctcc tgcgaaacgg tgaaacggct gttgggacgg cgggcagcgg gcgcgatgcc
     1741 gcgtccgcat gctttctgcc cgccatgctg caatgcggga aatggtctag ctggttacct
     1801 gaaactatct ggacgccggc aaccggcgcc cacatgcacc cacagtaacc gaaatttccg
     1861 acagtttata caaactaact agatggtaca aattcgtaaa aaccccgaat gacgattgcg
     1921 gccaatgcgc gcacactgca ttcacgttgc agagccccgg cgcgtggctc gctttatgcc
     1981 ttaccttttg caggagtggt tgtcatgagt tttgcatcag tgctggtcct caacggaccg
     2041 aatctcaacc tgctcggcac gcgtgagccg gctttttatg gctcggaaac actcgacgac
     2101 gtcgcgaaac tttgccgcga cgcgggcgag cggctggatc tgtcggtcga cttctgccaa
     2161 tcgaacgctg aacatcaact catcgactgg ctgcatgcgg cgcgcaccaa ggtcgacggt
     2221 atcgtgatca atccggcggc ttacacgcat acctcggtgg cgattgccga cgcgctcacc
     2281 gcgatcgaaa agcccgtcat cgaagtgcat atttcaaacg tgcatcgccg cgaagcgttc
     2341 cggcatcact cgtacgtgtc ggcggtggcc gaggcgatca tcatcggttg cggcacgcaa
     2401 ggctatgtgc tcgctctgga acggatggcg accatcctta agaatagggc agctaaatga
     2461 acgcacaaga gcacacccag acgagcactc agacgagcac tcaggcgaac actcaggcga
     2521 acgctcaggc gagtacccag gcgagcacac gttcctggct cgtcggtctg atcggctcgg
     2581 gcatcggcgg atcgctcagc cccgcaatgc atgaggaaga gggcagcaaa ctcggcctgc
     2641 actacgtgta tcggcggatc gatctggaag cgttgaagct cgacaccacg gcgctcgccg
     2701 atctgctggt tgccgccgaa cgcatgggct acaacggact gaacatcacg tacccgtgca
     2761 agcaggccgt cattccgctg ctcgacgaat tgtccgatga cgcgcgcgca ctcggcgcgg
     2821 tcaacacggt gctgttcaag ggcggcaaac gtatcggcca caacaccgat tggtccggtt
     2881 tcgcgcgtgc gtttcaacgc ggcctgccgg acgtatcact tgagcgcgtc gtgcaactgg
     2941 gcgcgggcgg cgcgggcgcg gcggtcgcgc atgccgcgct gacgatgggc acgcaaacgc
     3001 tcacgctctt cgacgtggat gccgcgcgcg ccgcctcgct cgccgcggaa ttgcagaagc
     3061 gtttccctgc cgccaccgtc agcgccggca gttcgctggc taagtcgctg aacgagtcgc
     3121 tgaacgagtc gctgaatgag tcgctcgccg ccgccaacgg cctgattcac gcgacgccta
     3181 ccggcatggc gaaattgccg ggcttgccgt tgccggtcga attgctgcat cgcgctctgt
     3241 gggtcgcgga catcgtttat tttccgatcc gcaccgcgct gctgcaggcc gccgaagcgc
     3301 tcggctgccg cacgctgagc ggcggcggca tggcggtata tcaggcagtc gacgcgatgc
     3361 gcattttcac agggctcgaa cccgacgccg agcgggttta cacgcatttc cagtccttgt
     3421 tgcaacgccc cgaagaaagt cccgctgcgg gacgctaagc cggttgcaca ggcaccccaa
     3481 ctatccggta cgaaacccga ccgaggagac agaacctcca tgcagcaacc gatccaatcc
     3541 acgctcgccg acgcggcagg ccaaacgaac gccacggtga gcaccgcgcg ccgctcgaag
     3601 gcccgctacc agattctctc gctgctggcg gtcggcacca tgatcaacta tctggaccgc
     3661 actgtgctcg gcatcgcggc gccgcagctg accagggaac tcggcatcaa tgccgcgcta
     3721 atggggctgc tgttctccgt gttctcgtgg agctacgtgg tggcgcagat tccgggcggc
     3781 ctgtttctcg accgcttcgg cagcaaactc acgtatttcc tgtcgatgac gttctggtcg
     3841 ctgtgcacgc tggcgcaggg g
//
LOCUS       DATASH010000838         2570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1924480,
            whole genome shotgun sequence.
ACCESSION   DATASH010000838 DATASH010000000
VERSION     DATASH010000838.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1924480"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="VHV99_22490"
     CDS             complement(<1..689)
                     /locus_tag="VHV99_22490"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX3638194.1"
                     /translation="MPPHNTDDVRIRELKELTPPAHLIREFACDETVSDVIYNSRNAM
                     HRILHGMEDRLIVIIGPCSIHDTKAAMEYAGRLVEQRKRFAGELEIVMRVYFEKPRTT
                     VGWKGLINDPHMDNSFKINDGLRTARELLLRINELGLPAGTEYLDMISPQYIADLISW
                     GAIGARTTESQVHRELASGLSCPVGFKNGTDGNVKIAVDAIKAASQPHHFLSVTKGGH
                     SAIVSTAGNED"
     gene            complement(1094..2560)
                     /gene="tldD"
                     /locus_tag="VHV99_22495"
     CDS             complement(1094..2560)
                     /gene="tldD"
                     /locus_tag="VHV99_22495"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEX3638195.1"
                     /translation="MNIIEPGIRNLATAKDILLTPYGLDESLLTRTLAEIFTHKIDYA
                     DLYFQYTRSEAWSLEEGIVKSGSFSIDQGVGVRAVSGDRTAFAYSDDLSPEAIRQAAI
                     ATRAIAKAGGGKQKIKAASSLTGIAGRDLYLPSDPLHSLDATAKVKLLERIEQMARGR
                     DPRIQQVMAGLAGEYDVVLVARSDGGFAADIRPLVRVSVTVIAEQNGRREIGSGGGGG
                     RFDYAYFTDEVLSGYVDDAVHAALVNLDARPAPAGAMTVVLGPGWPGVLLHEAIGHGL
                     EGDFNRKGSSAFAGRIGERVAAKGVTVVDDGTLPNRRGSLNIDDEGNPTQCTTLIEDG
                     ILKGYIQDTLNARLMKMPVTGNARRESYAALPMPRMTNTYMLNGDKDPQEIIASVKNG
                     LYAVNFGGGQVDITNGKFVFSASEAYMIENGKVTYPVKGATLIGSGPESLKFVSMIGN
                     DMKLDSGVGVCGKEGQSVPVGVGQPTLRIDRMTVGGTA"
BASE COUNT          465 a          771 c          818 g          516 t
ORIGIN      
        1 cagtcctcgt tgccggcggt cgagacgatc gccgagtggc cgcccttcgt gaccgacagg
       61 aaatggtgcg gctgtgacgc ggccttgatt gcgtcgacgg cgatcttgac gttgccgtcc
      121 gtgccgttct tgaagccgac cgggcacgac aatcccgaag ccagttcacg gtgcacttgc
      181 gactcggtcg tacgcgcgcc gatcgcgccc cacgagatca gatcggcgat gtactgcggg
      241 ctgatcatgt cgaggtattc ggtgccggcc ggcaggccga gttcgttgat acgcagcagc
      301 agttcgcgcg ccgtgcgcag gccgtcgttg atcttgaagc tgttgtccat gtgcgggtcg
      361 ttgatgagac ccttccagcc caccgtcgta cgcggctttt cgaagtacac ccgcatcacg
      421 atttcgagtt cgccggcgaa gcgcttgcgc tgctcgacga gacgcccggc gtattccatc
      481 gccgccttcg tatcgtgaat cgagcacggt ccgatgatga cgatcagacg gtcttccatg
      541 ccgtgcagga tgcgatgcat tgcgttgcgc gagttgtaga tcacgtcgga caccgtttcg
      601 tcgcaagcga attcgcggat caggtgagcg ggcggcgtga gttctttcaa ttcgcggatg
      661 cggacatcgt cggtattgtg cgggggcatg cttgttctcc tgaattgggt catagcgtgt
      721 tcgcgcgggc aacgaaggct gcgatcagac agcaatcagg cagcgatgcc agggcggaca
      781 acaatcaaaa aagtttcaac ggcaaaaggt tcaaagacgt gaggcgaaaa aaaaccgcca
      841 ggctagctgg cggttttcgt gaattctgag tgtcttgcgt gcactaacac ccctctcgat
      901 ccgccagcgg tctgagatgc caaaaaaagt aaaagtaaaa cttggcggac atggcgaatt
      961 aggtcgctgg tcaaaaaggg tgagtgactt tataacccgg gcaagctcgg ttgacaagct
     1021 acagggtata aaaatgcgga aaatccgcat acttcgcgca ttgcatgcac gaagtgtgcg
     1081 tctacatcaa aaatcaggcg gtgccaccaa cggtcatccg gtcaatgcgc agggtcggtt
     1141 gaccgacgcc caccggcacg ctctggcctt ccttgccgca cacgccgacg cccgaatcga
     1201 gcttcatgtc attgccgatc atgctgacga atttgagcga ttccgggccg ctgccgatca
     1261 gcgtcgcgcc cttgaccggg taagtgacct tgccgttttc gatcatgtac gcctcggagg
     1321 ccgagaacac gaacttgccg ttcgtgatgt cgacctggcc gccgccgaag ttcaccgcgt
     1381 acaaaccgtt cttcacggaa gcgatgattt cctgcgggtc cttgtcgccg ttgagcatgt
     1441 acgtgttggt catgcgcggc atcggcagcg cggcgtacga ctcgcggcgc gcattgcccg
     1501 tgaccggcat cttcatcagg cgcgcgttca acgtgtcctg gatgtagccc ttcaggatgc
     1561 cgtcttcgat cagcgtcgtg cactgggtcg gattgccttc gtcgtcgatg ttcagcgagc
     1621 cgcggcggtt cggcagcgta ccgtcgtcga ccaccgtcac gcccttcgcc gcaacccgtt
     1681 cgccgattcg ccccgcgaat gccgacgatc ccttgcggtt gaagtcgcct tcgaggccgt
     1741 ggccgatcgc ttcgtgcagc agcacgccgg gccagcccgg tccgagcacc acggtcattg
     1801 cgccggccgg cgccgggcgg gcgtcgaggt tcaccaatgc cgcatgcacg gcgtcgtcaa
     1861 cgtaacccga cagtacttcg tcggtgaaat aggcatagtc gaagcggccg ccaccgccgc
     1921 cgctgccgat ttcgcggcgg ccgttctgct cagcgattac tgtgaccgag acgcggacga
     1981 gcggacggat atcggccgca aagccgccgt cgctacgcgc gaccagcacc acgtcgtatt
     2041 caccggcgag gcctgccatg acttgctgga tgcgcgggtc gcggccgcgc gccatctgtt
     2101 cgatacgctc gagcagcttg accttggcgg tggcgtcgag cgagtgcagc gggtcggagg
     2161 gcaggtacag atcgcgcccg gcaatgccgg tcagcgacga ggccgccttg atcttctgct
     2221 tgccgccgcc cgccttggcg atcgcccgcg tggcgatggc cgcctggcgg atcgcttcgg
     2281 gcgacaggtc gtccgaatag gcgaaggccg tgcggtcgcc cgacacggcg cgtacgccga
     2341 cgccctggtc gatgctgaag ctgcccgact tcacgatacc ttcctcgaga ctccacgctt
     2401 cactgcgcgt gtactggaag tacaggtcgg cgtagtcgat cttgtgcgtg aagatttcgg
     2461 cgagcgtgcg ggtgagcagg gattcgtcga gaccgtaggg cgtcaggagg atgtccttgg
     2521 cggtggcgag attacggata ccgggttcga tgatgttcat gcgaagtatg
//
LOCUS       DATASH010000839         5866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_734047,
            whole genome shotgun sequence.
ACCESSION   DATASH010000839 DATASH010000000
VERSION     DATASH010000839.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5866
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734047"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..322
                     /locus_tag="VHV99_22500"
     CDS             20..322
                     /locus_tag="VHV99_22500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638196.1"
                     /translation="MGEFVPDYLLREQAAVGALVVFGVLRIIGAMLAYERGWRISVVE
                     KCFIAAAVVYCVPQLFELLTHMYGSRAAGAELEGKAAGCAIALFCAPILGHRLGLE"
     gene            349..522
                     /locus_tag="VHV99_22505"
     CDS             349..522
                     /locus_tag="VHV99_22505"
                     /inference="COORDINATES: protein motif:HMM:NF021447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YodC family protein"
                     /protein_id="HEX3638197.1"
                     /translation="MRLGKRSASLPKPAYRAGDTVTLRSGGRPMSVTWTGPVLFAPDY
                     WLICQWFDDDGEL"
     gene            complement(728..1657)
                     /locus_tag="VHV99_22510"
     CDS             complement(728..1657)
                     /locus_tag="VHV99_22510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEX3638198.1"
                     /translation="MNFDGLWIGQVAMAALMNVAFAFAVGSALLGAWLAKDARAKIAP
                     ARPAWLRAQRSMLTASVVLVLADLGWLLYQAASMSGVALPSAFGVVPTVLTQTHVGYG
                     WSVAFAGALVLLGTAMASHTGMLRNALLWLAVIAIAAGKASLGHAADAGPLSAAIGMQ
                     TLHVLVTSVWGGLAMAAGLAVLPALGTSTARGLLIRTATQVSSVSVVAVGLVLITGVF
                     NAVRGSGGSFEAIQMSTWGHVLTLKLALVVLALVLGGLNRFSALPRLRRTASTMDAHT
                     FVNVLYLEALAMIGVFVAAAVLSHSVPAFAALG"
     gene            complement(1654..2031)
                     /gene="copC"
                     /locus_tag="VHV99_22515"
     CDS             complement(1654..2031)
                     /gene="copC"
                     /locus_tag="VHV99_22515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis periplasmic binding protein
                     CopC"
                     /protein_id="HEX3638199.1"
                     /translation="MKLFNFSRPALHALTFGAAALVMTSTAFAHAHLVSSEPAANAAV
                     AAPAEVTIHFTEPLEPAFSRIDLSDASGKPAAPAVSQVDKSDAKVMHLALPQLTAGRY
                     AVHWIAVATDGHRTQGNFAFVVQ"
     gene            2398..3129
                     /locus_tag="VHV99_22520"
     CDS             2398..3129
                     /locus_tag="VHV99_22520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX3638200.1"
                     /translation="MELRVSSRRIFRSLLALMLIGSAGVYSAAKAQTQPKAPDTMEAR
                     VQGCTACHGSHGQGTDNDYFPRLAGKPADYLYNQLQNFREGRRKYPPMNYLVTYLSDD
                     YLHQIATYFSQQRPPYPTPAKPTVSAAIVARGQQIVLNGDASRQIPACAACHGKALTG
                     MEPAIPGLVGLHSDYISAQLGAWRSGSRHAIAPDCMHTIATRLTDDDVNAVASWLATQ
                     TAPQNPVPAPARSMKTPLACGSEPQ"
     gene            3151..4449
                     /locus_tag="VHV99_22525"
     CDS             3151..4449
                     /locus_tag="VHV99_22525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017334110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX3638201.1"
                     /translation="MKRKSLFALSAVVVVAAAALVPVLWSGGDNLHNGTAMAATPADQ
                     AALIKKGEYLARAGDCIACHTVRGGKQFAGGLPMATPFGTMFTPNITPDDQYGIGKWT
                     QDDFYRAMHTGRSKDGSLLYPGFPFTSYTKVTRADSDAIYAYLRSVTPVNVASRPHEL
                     KFPFNQRNMLIGWRTLFFREGEYKADPTKSVEWNRGAYLIEGLGHCGMCHTSINAMGG
                     PVSSAAFAGGLIPLQNWYAPSLTSNKEAGLGDWETKDIADLLKTGVSNRGAVFGPMAE
                     VVHNSLQYMSDEDINAMATYLKTIPQKSEAPEPLQLETSEKFGGELLKQGQKIYADNC
                     AKCHAENGLGMPPSFPPLANNQSIQMPSAVNPIRMVLNGGYPPSTDGNPHPYGMPPFA
                     QALSNTEVAAVVTYIRMSWGNHGTAVSPQQVSDLRSAPLD"
     gene            4568..5188
                     /locus_tag="VHV99_22530"
     CDS             4568..5188
                     /locus_tag="VHV99_22530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009914721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEX3638202.1"
                     /translation="MHVGERFNSITHLVGAVLSVAGLAALVTMGALDGDAYKVVSFSV
                     YGAMLFVLYAISTLYHSVRNPRVKAVLQKCDHSAIYLLIAGSYTPFTLVTLRGPWGWS
                     LFGVSWGLAALGIVQELTLGRRTRSVSMVLYVLMGWLALVAVRPLVQALPAAGTAWLV
                     AGGVIYSAGIYFFINDERIRHGHGIWHLFVLAGSLCQFVSVARYVA"
     gene            5445..>5866
                     /locus_tag="VHV99_22535"
     CDS             5445..>5866
                     /locus_tag="VHV99_22535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX3638203.1"
                     /translation="MSFDSLGLSEPLVRAVHELGYTTPTPIQTQAIPAVLNGGDLLAG
                     AQTGTGKTAGFTLPILQRLNTMPAVNTASGKRVVRALILTPTRELAAQVEESVRAYGK
                     YLKLKSTVMFGGVGINPQIGALRSGVDIVVATPGRLLD"
BASE COUNT         1115 a         1946 c         1781 g         1024 t
ORIGIN      
        1 aatgcatcgg ggaaacatca tgggcgaatt cgttccagac tatctgctcc gcgaacaggc
       61 cgccgtcggc gcgctggtgg tgttcggcgt gcttcgcatc atcggcgcga tgctcgccta
      121 tgaacgcggc tggcgcatct cggtagtcga aaaatgcttt atcgccgccg cggtggtgta
      181 ctgcgtgccg cagctcttcg agctgttgac gcatatgtac ggctcacgcg ccgcaggcgc
      241 ggagctggaa ggcaaggcag cgggctgcgc gatcgcgctc ttctgtgcgc cgattcttgg
      301 acatcggctc ggcctcgaat aaccggtttc actcagcgga agcacaccat gcgactggga
      361 aagagatctg cgagccttcc aaaacctgcc taccgcgccg gcgacaccgt gacgctcagg
      421 tccggtggac gtccgatgag cgtcacctgg accgggccgg tgctgtttgc gccagactac
      481 tggttgatct gccagtggtt cgacgacgac ggcgaactgt agcaggagat gtttccggaa
      541 gagacactgg cacgggcgga caacacgctc gccgcctaga gggcagtaac ggatcagccg
      601 gccaccgccg ggcgaccgtg tgtattgcgc cgcacgcttt tgctgtgcgg cgctttttta
      661 tgttgcattg aagaagcaac ggcggcgcgc actgcaattc gcgcggctaa cctctcccaa
      721 cgccaactca acccaacgcg gcaaacgccg gcacgctgtg cgacaacacg gcagccgcga
      781 cgaacacgcc gatcatcgcc agcgcttcca gatacagcac gttgacgaag gtatgcgcgt
      841 ccatcgtcga ggccgtgcgg cgcaaacgcg gcagcgccga gaagcggttg agtccgccga
      901 gcaccagcgc aagcacgacc agcgcaagct tgagcgtcag cacatggccc caggtgctca
      961 tctggatcgc ttcgaacgag ccgcccgaac cgcgcactgc attgaagacg ccagtgatca
     1021 gcaccagccc gaccgccacc accgatacgc tcgacacctg cgtcgccgta cggatcagca
     1081 gcccacgcgc ggtcgaggtg ccgagcgcgg gcaataccgc gagccccgcc gccatcgcca
     1141 gcccgcccca cacgctcgtg accagcacat gcagcgtctg catgccgatc gccgccgaga
     1201 gcgggccggc atcggccgca tgcccgagcg acgccttgcc cgccgcgatc gcgatcaccg
     1261 cgagccacag cagcgcattg cgcagcatgc ccgtatgact ggccatcgcc gtgcccagca
     1321 acaccagcgc gccggcgaac gccacgctcc agccgtagcc gacatgcgtt tgcgtcaata
     1381 cagtcggcac cacgccgaat gcggacggca acgcgacgcc gctcatcgag gccgcctgat
     1441 acaacagcca cccgaggtcc gccagcacca gcacgacgct ggccgtcagc atcgaccgtt
     1501 gcgcgcgcaa ccaggcgggc cgcgccggcg cgatcttggc ccgtgcgtcc ttggcgagcc
     1561 acgcgccgag caacgccgaa ccgacggcaa acgcaaacgc gacgttcatg agcgcggcca
     1621 tggcgacctg cccgatccac agtccgtcga agttcattgg acgacgaacg cgaagttgcc
     1681 ttgagtacgg tggccgtccg tggcaaccgc gatccaatgc accgcatagc ggccggccgt
     1741 caactgcggc agcgcgaggt gcatcacctt tgcgtcactt ttgtcgactt gcgacacagc
     1801 cggcgcggcg ggcttgccgc ttgcgtcact cagatcgatc ctgctgaaag ccggctcgag
     1861 cggctcggtg aaatggatcg tcacttccgc gggagcggcc actgcggcat tggccgcagg
     1921 ctcgctcgac accagatgcg catgcgcaaa agccgtggac gtcatgacca gagccgcggc
     1981 gccaaacgtc aacgcatgca gcgccggacg ggaaaaattg aatagcttca tgtaaggccg
     2041 atagcttatg gaaagtaaaa cggtagacgt tgagccgggg gcgtccgcgt cggacagaca
     2101 ccggcgcgcc cggaagtgta cgcttcgatg gtccccgcgc gcgtcggttc aacctggttt
     2161 gttacaagcc gggcataaac gatatctgcg tccgccatcg cgaataccaa atcgagatgg
     2221 ccgactcgcc tgcccgcgcg atggcgcggc aaactgtcgc atcggcgtaa caacgggaac
     2281 cttcttcaag ctgacaaata gccgccatca cccttcaatg ttagaatgct gcgtcgccgc
     2341 ggcgccggct gcccttcaca ccgaaccgct cgaagttcgg ccaggtcccc gattttgatg
     2401 gagttacgcg tgtcttcaag acgcattttc cgctcgctgc ttgcccttat gctgatcggc
     2461 tccgcgggtg tctatagcgc tgcgaaagcg cagactcaac cgaaggcccc cgatacgatg
     2521 gaagcgcgcg tgcaaggttg cacggcctgt cacggctcgc atggtcaagg caccgacaac
     2581 gactacttcc cgcgtctggc gggcaagccg gcagactatc tgtacaacca gttgcagaac
     2641 ttccgcgaag ggcgccgcaa gtatccgccc atgaactacc tcgtcacgta tctctcggac
     2701 gactaccttc atcagatcgc cacatacttc tcgcagcaac gtccgccgta ccccacgccg
     2761 gccaagccga ccgtgtcggc ggctatcgtt gcgcgcggcc agcagatcgt gctgaacggc
     2821 gacgcatcca gacagattcc ggcctgcgcg gcttgccacg gcaaagcgct gaccggtatg
     2881 gaacccgcta tcccgggtct ggtcggcctg cattcggact acatcagcgc gcagctgggc
     2941 gcgtggcgtt cgggctcgcg tcatgccatc gcacctgatt gcatgcacac catcgccacc
     3001 cgtctgaccg acgacgacgt gaacgccgtc gcctcatggc ttgccacgca aacggcccca
     3061 caaaaccccg tgccggctcc ggcccgctcg atgaagactc cgcttgcctg cggcagcgaa
     3121 ccgcaataag gcaccgggag agacactcaa atgaaacgca agtctttgtt cgccctctcg
     3181 gcagtcgtcg tcgttgcggc tgccgcactc gttcccgtcc tgtggtcggg cggcgacaat
     3241 ctgcataacg gcacggccat ggcggcaacg cccgccgacc aggccgcgct gatcaagaag
     3301 ggcgagtacc tcgcccgcgc cggcgactgt atcgcctgcc acacggtgcg cggcggcaag
     3361 cagttcgccg gcggcctgcc catggctacg ccgttcggca cgatgttcac gccgaatatc
     3421 acgcctgacg accagtacgg tatcggcaaa tggacccagg acgacttcta tcgcgccatg
     3481 cacacggggc gttcgaaaga cggcagcctg ctgtaccccg gcttcccgtt cacgagctac
     3541 acgaaggtca cgcgcgccga ttcggacgcg atctacgcgt acctgcgctc ggtcacaccg
     3601 gtgaacgtcg cgagccgtcc gcacgaactg aaattcccgt tcaaccagcg caacatgctg
     3661 atcggctggc gcacgttgtt cttccgcgaa ggcgagtaca aggctgatcc gaccaagtcg
     3721 gtcgaatgga accgtggcgc atatctgatc gaaggcctcg gccactgcgg catgtgccac
     3781 acgtcgatca acgcaatggg cggcccggtc agctcggcgg cattcgccgg cggcttgatc
     3841 ccgctgcaga actggtacgc accgtcgctg acgtcgaaca aggaagccgg cctcggcgac
     3901 tgggaaacca aagacatcgc cgatctgctg aagaccggtg tgtcgaaccg cggcgcggtg
     3961 ttcggtccga tggctgaagt ggttcacaac agcctgcagt acatgtcgga cgaagacatc
     4021 aacgcaatgg ccacgtacct gaagaccatt ccgcagaaga gcgaagcacc ggaaccgctg
     4081 cagctcgaga cttcggaaaa gttcggtggc gaactgttga agcaaggcca aaagatctac
     4141 gctgacaact gcgcgaagtg ccacgcggaa aacggcctcg gtatgccgcc gtccttcccg
     4201 ccgctcgcga acaaccagtc gatccagatg ccgtcggcgg tcaacccgat ccgtatggtg
     4261 ctgaacggcg gttatccgcc gagcacggac ggcaacccgc atccgtacgg catgccgccg
     4321 ttcgcgcagg cgctgtcgaa cacggaagtg gctgctgtcg tgacgtacat tcgtatgtcc
     4381 tggggcaacc acggcacggc cgtgtcgccg cagcaagtgt ctgatctgcg ttcggcgccg
     4441 ctcgactaaa cagcattaga agcacctggg gcgcggcctg cgaataacgc ggccgcgccc
     4501 ttcgtttttt caggaccggt atgatgtggg tattaaccct gatcaaaccg cgcaggagga
     4561 aagagccgtg catgtcggag agcgtttcaa cagcatcacc catctcgtcg gcgccgtgct
     4621 gtcggtggcg gggcttgctg cgctcgtgac gatgggcgcg ctcgacggcg acgcgtacaa
     4681 ggtggtgagt ttcagtgtct acggtgcaat gctgttcgtg ctgtatgcta tctcgacgct
     4741 ttaccacagc gtgcgcaacc cgcgcgtcaa agcggttctg caaaaatgcg atcattcggc
     4801 gatctacctg ctgatcgccg gcagctatac gccgttcacg ctcgtcacat tgcgcggacc
     4861 gtggggctgg tcgctattcg gcgtaagctg ggggcttgcc gctctcggca tcgtgcagga
     4921 actgacgctc gggcgacgca cccgcagcgt ttcgatggtg ctgtatgtct tgatgggatg
     4981 gctcgcgctc gttgccgtcc gcccgctggt tcaagcctta ccggcagcgg gtaccgcctg
     5041 gctcgtggcc ggcggggtca tctacagcgc cggcatctac ttcttcatca acgacgagcg
     5101 catccggcac ggacatggta tctggcacct gttcgtactg gcgggcagtc tgtgtcaatt
     5161 cgtcagtgtt gcgcgctacg tcgcgtgaca ccccacggcg gcgaaatgca acttaaggcc
     5221 agtcaacgtg tctgcatagc gcgttgaagc attcggcacg cgtttcccgc gtgccgccga
     5281 tttctctgcg accggaactg ggctttggcc tcgaacgcgc caagcgggtg tgttcatgcc
     5341 gtgcttgtgc ccgcttttag gcctgattcg ggccgcctgg gcccttttgg tgccgtctgg
     5401 tgccgtctgg tgccgttcgc gaaactgcat tgcaaagaac atttatgtct tttgattctc
     5461 tcggcttgtc cgaaccgctg gtccgcgctg tacacgaact cggctacacc accccgactc
     5521 cgatccagac ccaggcaatt cccgccgtgc tcaacggcgg cgatctgctg gccggcgcgc
     5581 agaccggcac cggcaagacc gccggcttca cgctgccgat cctgcagcgt ctgaacacca
     5641 tgcccgctgt caacacggct tcgggcaagc gcgtcgtgcg cgcgctgatc ctcacgccaa
     5701 cgcgtgagct ggccgcacag gtcgaagaaa gtgtgcgcgc ctacggcaag tacctgaagc
     5761 tcaagtcgac cgtgatgttc ggcggcgtcg gcatcaatcc gcagatcggt gcgctgcgca
     5821 gcggcgtgga catcgtcgtc gccacgccgg gccgtttgct cgacca
//
LOCUS       DATASH010000840         3042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1330949,
            whole genome shotgun sequence.
ACCESSION   DATASH010000840 DATASH010000000
VERSION     DATASH010000840.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1330949"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..94)
                     /locus_tag="VHV99_22540"
     CDS             complement(<1..94)
                     /locus_tag="VHV99_22540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX3638204.1"
                     /translation="MATLTPYSSQAPSRAEVDTLPGVTVMEFGTN"
     gene            319..699
                     /locus_tag="VHV99_22545"
     CDS             319..699
                     /locus_tag="VHV99_22545"
                     /inference="COORDINATES: protein motif:HMM:NF022400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HEX3638205.1"
                     /translation="MANKIYLRHPASDDTVAVATGFSWGALLLGFVWAMSKRMWFAAF
                     IMLAVNTLLFFSGLWGDTIGTIGLVLSVLFGIACGVYGNQWHRSALEQRGYVVVPPAG
                     EGSLTGADVAKAQATPARPPKQVP"
     gene            845..1318
                     /locus_tag="VHV99_22550"
     CDS             845..1318
                     /locus_tag="VHV99_22550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638206.1"
                     /translation="MLRRGLRCVAVIASLCAAPLAHSADPAAAPAPGVPVTPAAPGMQ
                     AAMPAAGSAGAPDDTFLLTIFLRHDESKPLPKINDQLRAQGFFKTFPPPGIEVVSWYV
                     MMGIGQVVTLRVPASRLREVNRAIETTAWGGYRTEFYPTYDYKAQAQKMRATDAK"
     gene            1492..2814
                     /locus_tag="VHV99_22555"
     CDS             1492..2814
                     /locus_tag="VHV99_22555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HEX3638207.1"
                     /translation="MLKTSRFFDLLHIPGFKPLAAATLMLGVAMSFTAPYLSLFGVER
                     AGMTPLKLGIFMTLIAASGVLASTFAGRWSDASGRHRPLLLAALVAAALGYLCLCVVR
                     DYRLLLAVGIVFIGAGGSAISMVFSFSRAALPVPDPAERAFASATLRTILSAAWVFGP
                     SVGALVLAGAGFYGLFLFAAASFATCATIVWRMREPQGHLGDHTVEDTASEPSASISV
                     PPLTAPGEDAHEHLPGVASPNDIRRAVAALTLLGLAANATMIVLPLYIVHGLNGTHLD
                     VSVMLGLGALMEIPMMLALGAKSSTLHKPNWLAACAAVHAVYFVCMSLAGTVHVLIPM
                     QMLNAFVVAVTSCLGMVYVQDLMPRAPGRATALFFNAARVGSILSGVLSGLLVQAFSY
                     RGTFLFCGLLALCALVLFAVPGYRYPQMWRAVRRFARTQYGKLQARRR"
BASE COUNT          427 a          983 c          997 g          635 t
ORIGIN      
        1 agttcgtgcc gaattccatc accgtgacgc cgggcagggt gtcgacctcg gctcgcgagg
       61 gcgcttgaga ggagtagggt gtcagggttg ccatgggtag ggtgctccga tgcttttact
      121 gtcgccgaca cgggttttcg cttgcgcggc gccctgcagc gtgtgatggg gtaaagagta
      181 gcagctatgg ctgggttcgt cagtggggcg ggcgcgtgcc tggccgcgca aatttcacac
      241 aaacacatgc cgttgtcagc gcggcatgtt aaacatcacg attcgcccta gcggcaccct
      301 cgcgttcagg aaaatggaat ggcaaacaag atctaccttc ggcatccggc cagcgacgac
      361 accgtggcgg tcgctaccgg cttcagttgg ggcgcgttgc tgttgggctt cgtctgggcc
      421 atgtcgaaga gaatgtggtt tgccgctttc atcatgctgg cagtcaatac gctgctgttt
      481 ttctccggct tgtggggcga tacgatcggc acgatcggac tggtgctgtc cgtgctgttc
      541 ggcattgcct gtggtgtcta tggcaaccag tggcaccgct cggcgctcga acagcgcgga
      601 tacgtcgtcg tgccgccggc cggcgagggc agcctgaccg gagcggatgt tgctaaagct
      661 caagccactc ccgcgcgtcc acctaagcaa gtcccctgaa gtacgcagcc gagtttgaag
      721 tacgcagtcg agtcctttcc cgccgccaag gagccatccg atgccgattg ccattgccat
      781 tgccattgcc ttaagcacgt ccaccccgtc tgtcccatct gtccgatctg tcacgcctat
      841 cgccatgctg cgccgcggct tgcgttgcgt ggcagtcatc gcctccctct gtgccgcgcc
      901 gcttgcgcac agcgccgacc ctgccgccgc gccggcgccg ggcgtgccgg tcaccccagc
      961 cgcacccggc atgcaagccg ccatgcccgc cgcaggctcg gccggcgcgc ccgacgacac
     1021 cttcctgctc accatcttcc tgcgtcacga cgagtcgaag ccgctgccga aaatcaacga
     1081 ccagttgcgc gcgcaaggat tcttcaagac ctttccgccg ccgggcatcg aggtcgtgtc
     1141 gtggtacgtg atgatgggca taggccaggt ggtcacgctg cgcgtgccgg ccagccgtct
     1201 gcgtgaggtc aatcgggcta tcgagacgac ggcatggggc ggctatcgca ccgagtttta
     1261 tccgacctac gattacaagg cgcaagcgca gaaaatgcgc gcaacggacg ccaagtagcg
     1321 gcgtctgcgt cgccggcgcg ttggcaatgg cgcgcaacgc gcttttggga taggggattg
     1381 cgggtttacg tgcccgcacg tcaggacgat aatgccccgg tcgttccggc cacgaagtca
     1441 gcggcgcgca gcgcgcgttg ccgacccgct tcccctgacc ctgcgcccct cgtgttgaaa
     1501 acctcccgtt ttttcgacct gctgcacatc cctggcttca agccgctggc cgccgccacc
     1561 ctcatgctcg gggtggcgat gtcgttcacc gctccgtatc tgtcgctgtt cggtgtcgag
     1621 cgcgccggca tgacgccgct caagctcggc atcttcatga cgctgatcgc cgccagcggg
     1681 gtgctcgcca gcaccttcgc cggccgttgg agcgatgcga gcggacggca tcggccattg
     1741 ttgctggcgg cactggtggc cgccgcactc ggctatctct gtctatgcgt ggtgcgtgac
     1801 tatcgcttgc tgctcgcggt cggcattgtc tttatcggcg cgggcggctc ggccatttcg
     1861 atggtgttct cgttcagccg cgcggcgctg ccggtgccgg accccgcgga acgcgcgttc
     1921 gccagcgcca ccttgcggac cattctttcg gcggcgtggg tattcggtcc gtcggtgggg
     1981 gcgctggtgc tcgccggcgc cggcttctac gggctcttcc tgttcgcggc ggcgagtttc
     2041 gcgacctgcg ccaccatcgt ctggcgtatg cgtgagccgc aaggccatct gggcgatcac
     2101 acggtggaag acacggcgtc ggagccgtcc gcctcgatca gtgtgccgcc gctgaccgcg
     2161 cccggcgaag atgcccacga gcacctgccg ggcgtcgcct cgccgaacga cattcgccgt
     2221 gccgtggccg cgcttacctt gctcggtctc gccgccaatg ccacgatgat cgtgttgccg
     2281 ctttacatcg tgcacggtct gaacggcacg catctggacg tgtcggtcat gctcggtctc
     2341 ggcgccttga tggagattcc gatgatgctc gcgctcggcg cgaagtcgtc gacgttgcac
     2401 aagcccaact ggctggccgc ctgcgccgcg gttcacgcgg tgtatttcgt ctgcatgtcg
     2461 ctggcgggga ctgtccacgt gctgattcca atgcaaatgc tcaatgcgtt cgtggtcgcg
     2521 gtgacctcct gcctcgggat ggtctacgtg caggatctga tgccgcgggc gccgggccgc
     2581 gcgaccgcgc tgttcttcaa cgcggcgcgg gtcggttcga ttctctccgg tgtattgtcg
     2641 ggtttgctgg tccaggcatt cagctatcgc ggcaccttcc tgttctgcgg cttgctggcg
     2701 ctttgcgcgc tggtgctgtt cgccgtgccg ggttaccgct atccgcaaat gtggcgcgcg
     2761 gttcggcgtt tcgcgcgcac gcaatatggg aagctgcagg cacgcaggcg atagggcctg
     2821 cggcgtcgag gcgatcgcgg gttatctgtt gtcgctgcaa atgccggggc gcgttcagtc
     2881 gagcgcgctg ccggcgcaag ccgtatcacc agtttcgcct ggagcacgta tccgatcatg
     2941 cggttttcgt cgatgccgaa ggtgctcgaa gtccatctga tcggccggcc cggcttaccg
     3001 tttccgggtg tcggtgaagc ggcgcagggg ccgggcgcgg cc
//
LOCUS       DATASH010000841         2635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5684082,
            whole genome shotgun sequence.
ACCESSION   DATASH010000841 DATASH010000000
VERSION     DATASH010000841.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5684082"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      complement(74..237)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     regulatory      complement(270..371)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(514..867)
                     /locus_tag="VHV99_22560"
     CDS             complement(514..867)
                     /locus_tag="VHV99_22560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638208.1"
                     /translation="MPRIVRVVAACALLTSLAACVVAPQQSAPAPRHNPQQIADQRLR
                     QVDGRIDNLSRHIDAHVNQGYYPPAQGAELHHRLDVIRQEAHEMAARHGGGLSSDEQR
                     LLNQELDNAAHAIGE"
     gene            complement(1070..2119)
                     /locus_tag="VHV99_22565"
     CDS             complement(1070..2119)
                     /locus_tag="VHV99_22565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEX3638209.1"
                     /translation="MSASLKIGLMGYGFAGATFHAPVIEHCGRASIAAIATSQPEHAL
                     ADYPHAKVVADLDALLALEEIDCVVIATPNDTHFDLARRTLEAGKHVVVDKPVTLTAA
                     DAHTLANIALVRSKLFVPFHNRRWDGDFLTVRDLLASRELGRITQYESHFDRFRPEVR
                     QRWREEASRGGGLLLDLGPHLIDQALALFGAPQTVSATVRTHREQASAPDYVHMQLGY
                     GEFEVVLHASALTALVAPRFAIHGTHGSYVKYGLDTQEDQLKAGLRPGDEGFGAGNAA
                     GVLRVLEGDQEVEREVPTRNGEYVGFYIALADAIQNGVKFPVTPQDAVDVMTVIELAA
                     RSSEEGVRLPFERIR"
BASE COUNT          464 a          818 c          832 g          521 t
ORIGIN      
        1 gggcatctcg cgaaacaaaa aaggccatgc ggcactacgc ctcgcggcct gctggctgcg
       61 agcgcttgcg ttgcatgacc cctctgtcct cgatacctga gagattgcgc tgccgggcga
      121 tttaaatgct gctgcaatcg ctgcggtgga acgcgcgccc cttcggtggg cagcctgcca
      181 aagtctgctc gatataaacg acgcagacgg ctactgccgc tctccagagt gcgaaaaacc
      241 caagcctgat gttgcgttga cgtgggtttt tcgacgcggt cggtcctttt gcctgagagt
      301 ttgcgggtgt gccccttcgg cggcgcggcc tgcgattttc tgcggccagc acgctctccc
      361 gacgcgtgcg gcgaatgtac gcgagggccg ggggcttgtc aaataaaggc ctgcgagggc
      421 catgggggtt ggcgccacag gtgcgcggaa atcaaaaaaa agcgcgggac gggctgcccg
      481 cgcttctctg cttcggaacc tgaaccggcg cgattattcg ccgatggcat gcgccgcgtt
      541 gtcgagttcc tgattcagca ggcgctgttc gtcgctggac agaccgccgc catgtcgcgc
      601 tgccatctca tgagcttcct gacggatcac atcgaggcgg tgatgcaact cggcaccttg
      661 cgcaggcggg tagtagcctt ggttgacgtg agcgtcgatg tggcggctca ggttgtcgat
      721 acggccgtcg acctgacgca gacgctgatc cgcgatttgc tgcggattat ggcgcggcgc
      781 cggcgcggat tgctgggggg ccaccacgca ggcggcgagg gaggtgagca atgcgcatgc
      841 tgctactacg cggacgatac ggggcatgga ctctccggga agtcgttgtc gttttgtggt
      901 gctacagggt agcaacggat tgccggagat tcacgacgac cgcgaagacg gggttatttg
      961 taacgttatg ttcctttccg ccttgtcagg catttcgtct ctggcgagag catgccgatg
     1021 ccgtggatgc ccccgccaga tagcccgcaa gcgccccgct ggcaaaaaat cagcgaatac
     1081 gctcaaacgg cagccggacg ccttcttccg aactccgggc agcaagctcg ataaccgtca
     1141 tcacgtcgac ggcatcctgt ggggtgacag gaaatttcac gccgttctga atggcatccg
     1201 ccaaggcgat atagaagccg acatattctc cgttgcgcgt cggtacttcc cgctcgactt
     1261 cctgatcgcc ttcaagcacg cgcaagacac ccgccgcatt gccggcgccg aagccctcgt
     1321 cgccgggccg caaaccggct ttcaactgat cttcctgcgt atcgagtccg tatttcacat
     1381 agctcccatg ggtgccgtga atcgcgaacc tcggtgcgac gagcgcagtc aacgcgctgg
     1441 catgcaacac cacttcgaac tcgccatatc ccaactgcat atggacgtag tccggcgcgc
     1501 tggcctgctc gcgatgcgtc cgaaccgttg ccgatacggt ctgcggcgcg ccgaacaacg
     1561 ccaacgcctg atcgatcaga tgcggcccaa ggtccaatag cagaccgccg ccgcgcgaag
     1621 cctcctcgcg ccaacgctgg cggacttccg gccggaaacg gtcgaaatgc gattcgtatt
     1681 gcgtgatgcg ccccagctcc cggctggcaa gcagatcgcg cacggtcagg aaatcaccgt
     1741 cccagcggcg gttgtgaaag ggcacgaaca gcttgctgcg gacgagcgca atgttggcga
     1801 gggtgtgggc gtcggccgcg gtgagcgtga ccggtttgtc gaccaccacg tgcttgcccg
     1861 cttccagcgt gcggcgtgcc aggtcgaagt gcgtgtcatt gggcgtggcg atgacgacgc
     1921 agtcgatttc ctcgagcgcc agcagcgcgt cgagatcggc caccaccttc gcgtgcgggt
     1981 aatcggccag cgcatgctcg ggctggctgg tcgcgatggc cgcgatgctc gcgcgcccgc
     2041 agtgctcgat cactggcgcg tgaaaggtcg cgccggcaaa accgtaaccc atcaatccaa
     2101 tcttcagcga tgcggacatg cgtgtcttcc tgcaaaaacg gcgcgccgct ttgccaccgt
     2161 ggcgacggcg cgcaacactg taattgtggc atgtcatgaa ggtggactgt cagcgttgtg
     2221 gtaggtgtct gtcagtgggg tcagtctgga cggcgttcgg ctgcccgttg cgacagcagt
     2281 cgggctagcg ggttcgagca tgcgtttttg tcgtcgggcg agcagcgatc gcggacatct
     2341 tttcgtcatc tgtcgtggca tccgggacag acatccgtcg cagtcatttc tgtcgcagcc
     2401 atccgtgtta acatcgctcc tctcgcgcgg gaacgcagcg ggccggaacg cctccgtacc
     2461 acctggggcg cgtgtttcag ccgctggcgc cgcgcagcat gcccccgggc cttcggtctc
     2521 gcgatttcgc acctaagcgc cctcaaccgc attacccatc cactgacgat gtccgcaggc
     2581 ctgaaccccg ctcaaaatga agcggtccgt tatcttgacg gtccgtgtct cgtgc
//
LOCUS       DATASH010000842         4356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3703255,
            whole genome shotgun sequence.
ACCESSION   DATASH010000842 DATASH010000000
VERSION     DATASH010000842.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4356
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3703255"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="VHV99_22570"
     CDS             <1..688
                     /locus_tag="VHV99_22570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX3638210.1"
                     /translation="FQVLTDCTTEVKKGEVVVVCGPSGSGKSTLIKTVNGLEPFQKGE
                     IVINGQSLTDKKTNLSKLRAKVGMVFQHFELFPHLSIVQNLTLAQIKVLGRSKDEATT
                     KGYKLLDRVGLRAHADKFPGQLSGGQQQRVAIARALSMDPIAMLFDEPTSALDPEMIN
                     EVLDVMVELAQEGMTMMCVTHEMGFAKKVAHRVIFMDKGLIVEDDRKEDFFANPKSDR
                     AKDFLAKILH"
     gene            complement(921..1853)
                     /locus_tag="VHV99_22575"
     CDS             complement(921..1853)
                     /locus_tag="VHV99_22575"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEX3638211.1"
                     /translation="MCQLLGMNCAAPTDVTFSFTGFAARGGVTDHHADGWGIAFFEDK
                     ACRLFIDHQSSATSPIAEMVKRYPIKSKNTIAHIRKATQGHILLENCHPFMRELWGRH
                     WIFAHNGDLKDYSPALNGVYQPVGTTDSELAFCALLQGLRKAFPGCQPPLEELFEALE
                     TLTREITQFGVFNFLMSNGQALFAHCSTHLHYIVRRWPFSTAHLVDADVSIDFAKYTT
                     PEDRVAVIATKPLTDNEVWTAFQPGDLMMFQHGDVIGRVNVPVPFSVQEKLRNPALDA
                     SASATTIAASVGAVATADLGVDLEAADDTAAFES"
     gene            2033..3103
                     /gene="pyrC"
                     /locus_tag="VHV99_22580"
     CDS             2033..3103
                     /gene="pyrC"
                     /locus_tag="VHV99_22580"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX3638212.1"
                     /translation="MTASNASPDSIDTVTLARPDDWHLHVRDGEMLAAVLPDTARQFG
                     RAIIMPNLKPPVTTTAMAEAYRERIVAAIPAGAKFEPLMTLYLTDNTPPDEIRRARES
                     GFVHGLKLYPAGATTNSDAGVTDIMKCAKTLEVMQETGMPLLVHGEVTDSSIDLFDRE
                     KVFIDRVMTPLRRAFPALKVVFEHITTKDAVDYIREAGVAPGLLGATITAHHLLYNRN
                     AIFQGGIRPHYYCLPVLKRETHRVALVEAATSGNPRFFLGTDSAPHPKGLKEHACGCA
                     GCYTALHALELYTEAFDKAGALDKLEGFASFFGADFYGLPRSAEKVTLRREEWMLPAE
                     LPVGDTPVVPLRGGESIGWRLV"
     gene            3115..3948
                     /locus_tag="VHV99_22585"
     CDS             3115..3948
                     /locus_tag="VHV99_22585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3025 domain-containing protein"
                     /protein_id="HEX3638213.1"
                     /translation="MQVQEATRVDFGFAAIDWSKPWFAQFAARGERWQQRALTSYAAL
                     LAEMNADAGEMQQVTGRGQRLAFIAQDDLPPGAAYEAHIASTGCVPTRHNLHDFFNGS
                     MWFAFPRIKAALNARQSAAIDASGVGPTRGGVRDTLTLFDENALLFACADPALSAALR
                     GFDWQTLLVAHRDAWGTRCEVRGFGHALLEKLVTPFKACTGHAWIVDVPSGYFGWDAA
                     ARDAWLDEAVSAALLGTEALTSRMFAPLPVLGIPGWWPENEVPAFYEDASVFRAGRRT
                     R"
     gene            4067..>4356
                     /gene="rnpB"
                     /locus_tag="VHV99_22590"
     ncRNA           4067..>4356
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VHV99_22590"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT          815 a         1288 c         1447 g          806 t
ORIGIN      
        1 gtttcaagtg ctgaccgact gcacgacgga agtcaaaaaa ggtgaagtgg tggtggtgtg
       61 cgggccgtcg ggctcgggca agtccacact gatcaagacc gtcaacggcc tcgagccgtt
      121 ccagaagggc gagatcgtca tcaacggcca atcgctgacc gacaagaaaa ccaacttgtc
      181 gaagttgcgt gcgaaggtcg gcatggtgtt ccagcacttc gaactgttcc cgcatctgtc
      241 gatcgtgcag aacctgacac tcgcgcagat caaggtgctt ggccgctcga aggacgaagc
      301 cacgaccaag ggctacaagc tgctcgatcg tgtcggtctg cgggcgcatg cggataagtt
      361 tccggggcag ttgtcgggcg gtcagcagca gcgtgtggcg attgcgcgcg cgttgtcgat
      421 ggaccccatc gcgatgctat tcgacgagcc gacctccgcg ctcgatccgg aaatgatcaa
      481 cgaagtgctc gacgtaatgg tcgaactcgc gcaggaaggc atgaccatga tgtgcgtcac
      541 gcacgaaatg ggctttgcca agaaggtcgc gcaccgcgtg atcttcatgg acaagggctt
      601 gatcgtggaa gacgaccgca aggaagattt cttcgcgaat ccgaagtcgg atcgtgcgaa
      661 ggattttctg gcgaagatcc tgcactgagg cttttgtcgg ggcacgctgc ttatgtggcg
      721 tggcccttgt agaaaaaaag ccgcttcatg ggatgaagcg gcttttttgg tttctgaagt
      781 tgagcttctg atgttcttcg caaacgaggc gcgggctgac cgttctgccg aacgtgaatc
      841 cggacggggc taacagcgct tgcgtttgcg aacgccgcgg tacggtcagc gttccggcat
      901 gacggggcgc acgcgccaga tcaggattcg aaagcggcgg tgtcgtccgc ggcttcgagg
      961 tcgaccccga ggtcggccgt ggccaccgcg ccaaccgaag ccgcaatcgt ggtggccgaa
     1021 gccgatgcgt ccagcgccgg gttgcgcagc ttctcctgca ccgaaaacgg caccggcacg
     1081 ttgacgcggc cgatcacgtc gccatgctgg aacatcatca ggtcgcccgg ctggaaggcg
     1141 gtccacactt cattatcggt gagcggcttg gtcgcgatca cggcgacgcg atcttccggc
     1201 gtcgtgtatt tggcgaaatc gatcgacacg tccgcatcga ccagatgagc ggtcgagaag
     1261 ggccagcgcc gcacgatgta gtgcagatgc gtcgagcaat gggcgaacag cgcctggccg
     1321 ttcgacatca ggaaattgaa cacgccgaat tgcgtgatct cgcgcgtgag cgtttcgagt
     1381 gcctcgaaca gttcttccag cggcggctgg caacccggaa aagccttgcg caacccttgc
     1441 agcagcgcgc agaacgcgag ctcgctatcg gtcgtgccga ctggctgata cacgccgttg
     1501 agcgccggcg aataatcttt caggtcgccg ttgtgcgcga agatccagtg gcgtccccag
     1561 agttcgcgca tgaacgggtg gcagttctcg agcaggatgt gcccttgcgt cgccttgcga
     1621 atatgcgcga tcgtgttctt cgatttaatc gggtagcgct tgaccatctc ggcgatcggc
     1681 gaggtcgccg atgactgatg atcgatgaac aggcggcacg ctttgtcttc gaagaaggcg
     1741 atgccccagc cgtcagcgtg gtgatcggtg acgccgccgc gcgccgcaaa gccggtgaac
     1801 gagaacgtga cgtccgtcgg cgcggcgcag ttcattccga gaagttggca catgtggcgg
     1861 gtaagcctgg gaacggggca agccgttaca atgatgattt tccaagcata tcaccgagcc
     1921 agaagcgcca aatagcaaaa ttgacgtgtg ttcaggccgc aacggtacga attgccgtcg
     1981 cgtgcccgcg tcgatcggcc ttttccccgc agaatctcgt tcccctctcg ccatgaccgc
     2041 ttccaacgcc tctcccgatt ccatcgacac cgttacgctc gcccgcccgg acgactggca
     2101 cctgcacgtt cgcgacggcg agatgctcgc ggccgtgctg ccggataccg ctcgccagtt
     2161 cggccgcgcg atcatcatgc ctaacctgaa gccgccggtc accacgacgg caatggccga
     2221 ggcgtaccgc gagcgcatcg tcgccgcgat cccggccggc gcgaagttcg aaccgctgat
     2281 gacgctgtac ctgaccgaca acacgccacc cgacgaaatc cgccgcgcgc gcgaaagcgg
     2341 cttcgtgcat ggcttgaagc tgtatccggc gggtgcgacc acgaactcgg acgcaggcgt
     2401 caccgacatc atgaagtgcg ccaaaacgct cgaagtgatg caggaaaccg gcatgccgtt
     2461 gttggtgcac ggcgaagtga cggattcgtc gatcgacctg ttcgatcgcg agaaagtgtt
     2521 catcgaccgc gtgatgacgc cgctgcgccg cgcgtttccg gcgctgaagg tggtgttcga
     2581 gcacatcacc acgaaagatg ccgtcgacta tatccgcgaa gctggcgtgg cgccggggct
     2641 gctgggtgcg acgattaccg cgcaccattt gctgtacaac cgcaacgcga tctttcaggg
     2701 cgggattcgc ccgcattact actgcctgcc ggtgttgaag cgcgagacgc atcgcgtggc
     2761 gctggtcgag gcggcgactt cgggcaatcc gcgcttcttt ctcggcaccg atagcgcgcc
     2821 gcatccgaag ggtttgaagg agcacgcgtg tggctgcgcg ggttgctaca cggcgctgca
     2881 tgcgctcgaa ctctatacgg aagcattcga caaagccggc gcgctggaca agctcgaggg
     2941 cttcgccagc ttcttcggcg cggacttcta tggtctgccg cgcagcgcgg agaaggtcac
     3001 gctgcgtcgc gaagaatgga tgctgccggc tgaattgccg gtcggtgaca cacctgttgt
     3061 gccgctgcgc ggcggtgagt cgattgggtg gcgcctcgtc tgaggctgcg ttagatgcag
     3121 gtgcaagagg cgacgcgcgt ggacttcggt ttcgccgcga ttgactggtc gaagccgtgg
     3181 ttcgcgcagt tcgcggcgcg cggcgagcgc tggcagcagc gcgcgctgac gagctacgcg
     3241 gccttgctcg cggaaatgaa tgccgatgcc ggcgagatgc agcaggtcac ggggcgcggt
     3301 cagcgcctcg cgttcattgc acaagacgat ctaccgccgg gcgcggctta tgaggcgcac
     3361 attgcgtcga cgggctgcgt gccgacccgc cataacctgc acgacttctt caatgggtcg
     3421 atgtggtttg cgtttccccg tatcaaggcg gcactgaacg cgcggcagtc ggcggcgatc
     3481 gacgcgtcag gtgtcggccc gacccgcggc ggcgtgcgtg acacgttgac cttgttcgac
     3541 gaaaacgcgt tgctgttcgc ctgtgccgat ccagcgttga gcgccgcgtt gcgcggattc
     3601 gactggcaga cgttgctggt tgcacatcgc gacgcctggg ggacgcgctg cgaagtgcgc
     3661 ggcttcggtc atgcgctgct ggaaaagctg gtcacgccgt tcaaggcgtg cacaggccac
     3721 gcgtggattg tcgacgtgcc gtccggatat ttcggctggg acgccgcggc gcgcgacgcc
     3781 tggctcgacg aggcggtgag cgcagcgctg ctgggcacgg aggcattgac aagccgtatg
     3841 ttcgcgccgc tccccgtgct gggcattcca ggatggtggc ctgagaatga ggtgccggcg
     3901 ttttacgagg atgcctcggt gtttcgagca ggccgccgca cacgctaggg gttgtcggct
     3961 cacggcgcgg cgcgagccga attgctcggg tcaggctagc cgccattcgg ctatttctct
     4021 cgccgacgcg gcttccatcc ttgcagatgt taaaatgcgc cccagcaaag caggccaggc
     4081 agtcgcggcc tccgcggttt cggccaaaga gggcgaggaa agtccggact ccatagggca
     4141 gggtgatggc taacggccat ccgtggtgac acgcggaata gggcaacaga aagcaaaccg
     4201 ccgatggccc ggcgcaagtc gggatcaggt aagggtgaaa cggtgcggta agagcgcacc
     4261 gcggctgctg caaggcagac cggcacggta acctccaccc ggagcaattc caagtaggca
     4321 ggcgcgcatc ttcgagatgc aggacggggc ccccgt
//
LOCUS       DATASH010000843         1556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2320795,
            whole genome shotgun sequence.
ACCESSION   DATASH010000843 DATASH010000000
VERSION     DATASH010000843.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2320795"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..689
                     /locus_tag="VHV99_22595"
                     /pseudo
     CDS             1..689
                     /locus_tag="VHV99_22595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018434454.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            957..1556
                     /locus_tag="VHV99_22600"
                     /pseudo
     CDS             957..1556
                     /locus_tag="VHV99_22600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018434454.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
BASE COUNT          281 a          517 c          502 g          256 t
ORIGIN      
        1 cgaccgtcgc ccatctcgcg gtgcacggct tgccgatctt tgtcgtcgac gacggcagcg
       61 atgaagcgac ccagcaggtg ctcgccgggt tggcgcggca gtacgccggg cagctcacgc
      121 tgctgcggtt gccggtcaac ggcggcaagg gcgctgctgt gatggcggga ctgcgcgccg
      181 cgcgcgctgc gaattacacg catgccttgc agatcgacgc cgacggccag cacgatgcgg
      241 ccgacgtgcc gcgttttatc gaagcggcac gcgccgagcc ggccgcggtg atcctcggcc
      301 gcccggtcta cgacgagagc gtgccgaaag cgcgcctcta tggccgctat ctgacgcacg
      361 tgtgggtgtg gatcgaaacg ctctcgctga cgattcgcga ttcgatgtgc ggtttccgcc
      421 tctatccgct tgcgttggcc tgcgagttga tcgacagcgt gcaactgccg acgcgcatgg
      481 atttcgacat tgaaattctc gtgcgcctgc actggcggcg cgcggcgttc cgctcgatgc
      541 cgacgcgtgt cacgtatgca gccgacggcg tttcgcattt cgacgtgctg tgggacaacg
      601 tgcgtatcag ccgcagtcac acgcggcttg tgttcgggat ggtgtggcgt ctgccgatgc
      661 tgctcgcgca caaggtgatg ccacgtcgcc cggcggtggc gagcgtgccg ggtgcagcgc
      721 gcgaacatgc caggcacgcc acgcagggca agcaggctaa ccatggcgag ccggctaatc
      781 atggcgaacg actcgaacaa ggcaggcctg gcgaaccaag cacgccagct gaaccagaca
      841 cacgagccga accagacgcg cgagccgaac cagacacgcg agccgaacca gacacgcgag
      901 ccgaaccaga cacgcaagcc gaaccacgcg cgccagccaa acaaaacgaa cagcccccgc
      961 aggactggtg gcgaatcgcc gaacgcggca gccatctggg catgtccttg ctcgcgctca
     1021 gttgcaagct gttcggccgc cgtttcaccg cgctctggtt gcatccgatc gttgcgtatt
     1081 ttctgctcac gggccgcgcc gcgcgcgagg catcgagcaa ctacttcacg cgaggcatcg
     1141 agcaactact tcacgcgtct tggcgaagcc gcgccgcacg gcgatacacc acgtccgggc
     1201 tggctatccg cctatcgtca catgctcgcg ttcgcgcaat ccggcttcga caagctcgcc
     1261 gcctggtccg ggcgcgtcaa caacgccgac gtcgaattcg aagatccttc ggcattcgaa
     1321 gcattggtgg caagcggcaa aggcgcgctc gtgatcggcg cgcatctcgg caatctggag
     1381 atgacccgcg cgcttgccgc gcagggcgcc tacgccaaag tcaccgccgt ggtgtacacc
     1441 gaacacgcgc ggcgcttcaa tagcgtgctg gcgtcggcca atagccagtt cgcgcggcac
     1501 ctgctcgaag tcagcgactt cggtcccgag accgcgatga tgatgcagga gcgtgt
//
LOCUS       DATASH010000844         2109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5285658,
            whole genome shotgun sequence.
ACCESSION   DATASH010000844 DATASH010000000
VERSION     DATASH010000844.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5285658"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="VHV99_22605"
     CDS             complement(<1..509)
                     /locus_tag="VHV99_22605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3638214.1"
                     /translation="MAKIFCVANQKGGVGKTTTAVNLAASLAAQGQRVLLIDLDPQGN
                     ATMGSGVDKAACANTVYEVLVDGVSVADARVRPEAVGYDMLPANRELAGAEVELVSVQ
                     NRERQLKAALAAVENEYDFILIDCPPALSLLTLNGLCAAHGVVIPMQCEYFALEGLSD
                     LVNTIKQVHA"
     gene            complement(601..1284)
                     /gene="rsmG"
                     /locus_tag="VHV99_22610"
     CDS             complement(601..1284)
                     /gene="rsmG"
                     /locus_tag="VHV99_22610"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEX3638215.1"
                     /translation="MTVSQKGRDSEALAPLLADGVRELGLVLSDAQLGKLLDYVALLS
                     KWNAVYNLTAIRDPRQMLIQHILDSLSIVPHLATRGVSSVLDVGSGGGLPGIVLAIVL
                     PDWTVTVNDIVHKKTAFQAQAKAQLGLANLSVVTGRVETLQPGAEVPAKFDVIVSRAF
                     AELADFVTLARHLVADKGAIWAMKGVRPDGEIERLPAGAHVEQIIRLNVPSLDAERHL
                     IKVRVDAES"
     gene            complement(1281..>2109)
                     /gene="gidA"
                     /locus_tag="VHV99_22615"
     CDS             complement(1281..>2109)
                     /gene="gidA"
                     /locus_tag="VHV99_22615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012402386.1"
                     /note="GidA; glucose-inhibited cell division protein A;
                     involved in the 5-carboxymethylaminomethyl modification
                     (mnm(5)s(2)U) of the wobble uridine base in some tRNAs;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEX3638216.1"
                     /translation="YEEAAAQGLLAGINAGLQVQGEDAWCPRRDQAYLGVLVDDLVTR
                     GVSEPYRMFTSRAEYRLSLREDNADMRLTEIGRELGVVDDIRWDTFSRKRDAVSRETE
                     RLRTTWVNPKTLPAEEATALLGKPIDHEYSLADLLRRPGVTYDGVCGLRAGACAAPDT
                     LAEDDVLLAQIKEQIEIGIKYQGYIDRQADEIERNESHESTRLPDGLDYAEVRGLSFE
                     ARQKLTQFRPETIGQASRISGITPAAISLLMVHLKRGLGRRSTKPAEPGTDSTPVTQ"
BASE COUNT          394 a          681 c          610 g          424 t
ORIGIN      
        1 gcgtgcacct gcttgatcgt gttcaccaga tcggacagac cctccagggc gaagtactcg
       61 cactgcatcg gaattaccac gccgtgcgcg gcgcacaagc cgttgagcgt cagcagcgac
      121 aaagccggcg ggcagtcgat caggatgaaa tcgtattcat tctcgacggc cgccaaggcg
      181 gccttcaatt ggcgctcacg gttctgcacg ctgaccagtt caacctcggc gccagccagt
      241 tcacggttgg cgggcagcat gtcgtaaccg acggcttccg ggcgtactcg cgcatcggcc
      301 accgatacgc catccaccag cacttcgtag acggtgtttg cgcatgcagc cttgtcgacg
      361 ccgctgccca tcgtggcgtt gccctgcggg tccagatcga tgagaaggac tcgctgtccc
      421 tgcgctgcca ggctcgcggc cagattgact gccgtcgtcg tcttgccgac gccgcccttc
      481 tggttcgcaa cgcagaagat ttttgccatc gttggtgtgt cccttttccc tttttgctgc
      541 ttgaataaag tagaaagcgc ttctggtacg cggtgcgcgt cctggatgtg gcggtggccg
      601 tcagctttcg gcgtccaccc gcaccttgat caggtgccgc tccgcgtcga gcgatggcac
      661 gttcagccga ataatctgct ccacgtgggc gcccgctggc aggcgctcga tctcaccatc
      721 cgggcgtaca cccttcatcg cccaaatcgc gcccttgtcc gcaaccagat gacgggccag
      781 tgtaacgaaa tccgcgagct ctgcgaatgc gcgcgacacg attacgtcga actttgccgg
      841 tacttcggcg cccggctgca aagtctcgac gcgtcccgtg acgaccgaca ggttcgccaa
      901 gccgagctgg gctttggcct gggcctgaaa agccgtcttc ttgtgaacga tgtcgttgac
      961 ggtgaccgtc cagtcgggca agacgatcgc cagcacgatg ccaggcaggc caccgccgga
     1021 acccacgtcc agcaccgagg acacaccgcg cgtggcgaga tgcggcacga tcgaaagcga
     1081 atccaggatg tgctgaataa gcatctgccg gggatctcgg atcgccgtca ggttgtagac
     1141 ggcgttccat ttggacagca acgctacgta gtcgagcagt ttgccgagct gcgcgtcgct
     1201 taaaacgagg ccgagttcgc ggacgccgtc cgctagcagc ggcgccaatg cctcactatc
     1261 ccttcccttc tgactcaccg tcattgcgtc accggcgtgc tatcggtgcc gggttcggcg
     1321 ggttttgtcg aacgacggcc caagccacgc ttcaagtgga ccatcagcaa ggagatggcc
     1381 gccggggtaa tgccggagat acgtgacgcc tgaccgatag tctcgggacg gaattgcgtc
     1441 aacttctgac gcgcctcgaa cgacagccct cttacctcgg cgtagtccaa tccatcaggc
     1501 aaacgcgtac tttcgtgtga ttcattccgc tcgatctcat cggcctggcg gtcgatataa
     1561 ccctgatact tgatgccgat ctcgatctgc tccttgattt gggcgagcag aacgtcgtcc
     1621 tcagccagcg tgtcgggcgc agcacatgca cccgcgcgca aaccgcagac gccgtcgtag
     1681 gtcacaccgg gacggcgcag cagatcggcc agactgtatt cgtggtcgat cggcttgccg
     1741 aggagtgcgg tcgcttcttc ggctggcaac gtcttcgggt tcacccacgt cgtccgaagg
     1801 cgctctgttt cacgtgaaac agcgtcgcgc ttgcggctga atgtatccca gcggatatcg
     1861 tcgaccacac cgagctcacg accgatctcc gtcagacgca tatcggcgtt atcttcgcgc
     1921 aaactcagac ggtattcggc acggctcgtg aacatgcgat acggctcgga aaccccgcgt
     1981 gtgaccaggt catcgactaa aacacccaaa tacgcctgat cacggcgcgg gcaccatgcg
     2041 tcctcgcctt gtacctgcaa gcccgcattg attcccgcca gcaaaccttg tgcggcagcc
     2101 tcttcatag
//
LOCUS       DATASH010000845         2449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6077344,
            whole genome shotgun sequence.
ACCESSION   DATASH010000845 DATASH010000000
VERSION     DATASH010000845.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6077344"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VHV99_22620"
     CDS             complement(<1..184)
                     /locus_tag="VHV99_22620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008342986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HEX3638217.1"
                     /translation="MKHIVKHIHFVGIGGVGMSGIAEVLLNLGYQVSGSDLSSNGVTD
                     RLITLGARVAIGHREEN"
     gene            complement(181..1326)
                     /gene="murG"
                     /locus_tag="VHV99_22625"
     CDS             complement(181..1326)
                     /gene="murG"
                     /locus_tag="VHV99_22625"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEX3638218.1"
                     /translation="MTVSTSSRAGTLMVMAGGTGGHVFPGLAVAHWMQARGWRVVWLG
                     NASGMEATLVPKHGIAMEYVRFGGVRGKGLKTKLMLPVNLLRACSQSLHALRKVKPDV
                     VLGMGGYITFPAGLMSKLSGTPLVLHEQNSIAGLANRVLAHIAKRVLVAFPNALPNAE
                     WTGNPIRAELGEALPPKERYAARNVSHEARLNVLVVGGSLGAAALNETVPKALALLDP
                     ALRPRVVHQAGAKHIDALKANYAASGLSADGDVQLVPFIDDMASAYAAADLVICRSGA
                     MTVSEIAAVGVAALFVPFPFAVDDHQTTNAAFLANSGAAHLIQQRDLSAEKLAGWLRE
                     QTREKLASMAEKARALAKPDATERVGRVCADVAGLSALIQAPKEIQP"
     gene            complement(1323..>2449)
                     /gene="ftsW"
                     /locus_tag="VHV99_22630"
     CDS             complement(1323..>2449)
                     /gene="ftsW"
                     /locus_tag="VHV99_22630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEX3638219.1"
                     /translation="YDHSLLWVVVALLGLGIVMVYSASIAMPDSPKYAQYHDYFFLVR
                     HLVSIATAVVASIIAFKIPVSTWDKYAAKFFLFSLFLLVIVLIPHIGKGVNGARRWIP
                     LGITNMQPSEIMKLAVTIYAANYTVRKQEFMHSIGRGFLPMAVAVGFVGALLLLEPDM
                     GAFMVIAVIAMGVLFLGGVNAKLFGGLVATAVGTFALLVWASPWRRERIFAYLDPWDD
                     RYAQGKAYQLTHSLIAFGRGEWFGVGLGGSVEKLNYLPEAHTDFILAVIGEELGFVGV
                     LIVILLFYWIVRRAFEIGRQALALDRTFAGLMAKGIGLWFGAQTFINMGVNLGLLPTK
                     GLTLPLVSYGGSGILLNCVALAVLMRVDYENRVLMRGGKV"
BASE COUNT          522 a          815 c          696 g          416 t
ORIGIN      
        1 tattctcctc acgatgcccg atcgccacgc gcgcacccag cgtgatgagt cgatcggtca
       61 cgccgttgct ggatagatca gagccgctga cctgatagcc gaggttgagc aacacctcgg
      121 cgatgccgct catccccacg ccgccgatgc cgacaaagtg aatgtgctta acgatatgtt
      181 tcatggctgg atttccttcg gcgcttgtat cagcgcgctc aagccggcga catcggcgca
      241 gacccggccg acgcgttcag ttgcgtcggg tttcgccagg gcgcgagctt tctcagccat
      301 cgaggcaagc ttctcgcgag tctgttcgcg caaccagcca gcgagctttt ccgccgacaa
      361 atcgcgctgt tgtattaagt gggccgcgcc cgaatttgcg aggaacgcgg cgttggtggt
      421 ctggtggtca tcgacggcaa aggggaacgg cacgaacaac gccgccacgc caacggccgc
      481 gatctccgat acggtcatcg cacccgagcg gcagatcacg agatccgctg cggcgtaagc
      541 gctcgccata tcatcgataa acggcaccag ttgaacatcg ccatccgccg ataaacccga
      601 cgccgcgtag ttcgctttca acgcatcgat atgcttcgcg cccgcctgat gcacgacgcg
      661 cggacgcagc gccgggtcga gcaacgcgag cgctttcggt acggtttcat tcaaagcggc
      721 tgcacccaga ctgcccccga cgaccagaac attaagacgc gcttcatgac tcacattccg
      781 cgcggcgtag cgttctttgg gtggcaatgc ctcgccaagt tccgcacgaa ttggattgcc
      841 ggtccattca gcattcggca gcgcattcgg gaacgcgacc agcacgcgtt tggcgatatg
      901 cgcgagcacc ctgttcgcca gtcccgcgat tgaattctgt tcgtgcagca cgagcggcgt
      961 gccactcagc ttcgacatca aacccgccgg aaacgtgatg tatccgccca tgccaagcac
     1021 cacatccggc ttcaccttgc gcagcgcgtg caggctttgc gagcatgcgc gcagcaggtt
     1081 caccggcagc atcagcttcg tcttgaggcc cttgccgcgc acgccgccaa agcgcacgta
     1141 ttccatcgcg atgccgtgct tcggcacaag cgtcgcttcc atgcccgacg cattgccgag
     1201 ccacacgaca cgccagccgc gagcctgcat ccagtgagcg accgcgagcc ccgggaacac
     1261 gtgtcccccg gtgccgccgg ccatcaccat cagcgtgcct gcacgagatg acgtcgaaac
     1321 cgtcatacct tgcctccgcg catgagcacc cggttttcgt agtcgactcg catcagcacc
     1381 gcaagcgcga cacagttcag caagatgccc gagccgccgt agctcaccag cggcaacgtc
     1441 aggcctttgg tcggcaggag acccaggttc acgcccatgt tgatgaaggt ctgcgcaccg
     1501 aaccacaagc caataccctt cgccatcaaa ccggcaaacg tgcggtcgag cgcaagcgcc
     1561 tgacggccga tttcgaatgc gcgccgcaca atccagtaga acaggaggat cacgatcagc
     1621 acgccgacaa agccgagttc ctcgccgatc accgcgagga tgaagtcggt gtgcgcttcc
     1681 ggcagatagt tgagcttctc gacactgccg cccagtccca cgccgaacca ttcgccgcgg
     1741 ccgaacgcga tcagcgagtg cgtcaactga taggccttgc cttgtgcata gcggtcgtcc
     1801 cacggatcga ggtaagcaaa gatccgttca cgacgccacg gcgatgccca cacgagcaac
     1861 gcaaacgtgc caacggccgt tgccaccaga ccgccgaaca gcttggcgtt cacgccgcca
     1921 aggaacagta cgcccatcgc gatgaccgcg atcaccatga aagcgcccat gtccggctca
     1981 agcagcagca acgcgccgac gaagccgacg gcaacggcca tcggcaaaaa gccgcggccg
     2041 atgctatgca tgaactcttg cttgcgcacc gtgtagttgg ccgcgtagat ggtcacggcg
     2101 agcttcatga tttcggacgg ctgcatgttg gtgatgccga gcggaatcca gcgacgcgcg
     2161 ccgttcacgc ccttgccgat atgcggaatc agcacgatca ccagcaggaa cagcgagaac
     2221 aggaagaact tcgccgcgta cttgtcccac gtggacaccg gaatcttgaa cgcgatgatc
     2281 gacgccacga cggccgttgc tatcgataca agatggcgca ccaggaagaa gtagtcgtgg
     2341 tactgcgcat atttcggcga atccggcatg gcgatcgacg ccgaatacac catcacgatg
     2401 ccgagcccga gcagcgcgac gaccacccac aggagcgagt gatcgtagt
//
LOCUS       DATASH010000846         3362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6077625,
            whole genome shotgun sequence.
ACCESSION   DATASH010000846 DATASH010000000
VERSION     DATASH010000846.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6077625"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="VHV99_22635"
     CDS             <1..892
                     /locus_tag="VHV99_22635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX3638220.1"
                     /translation="ACATLMSVHNSVGCGPILNFGTDAQKDRYLQDLATGRRIGAFCL
                     TEPQAGSEANNLRTRAVLRDGKWILNGNKQFVTNGARANIAIVFAVTDPDRGKRGLSA
                     FIVPTDTPGFNVGKPEHKLGIRASDTCPISLDDCAVPEANLLGEPGEGLRIALSNLEG
                     GRIGIAAQAVGIARAAFDAARRYANERIQFGKALKDHQTIANMLADMATRLNAARLLV
                     HHAARLRTAGRPCLSEASQAKLFASELAEEICSNAIQIHGGYGYLEDYAVERHYRDAR
                     ITQIYEGTSEVQRMVIARHV"
     gene            1136..2827
                     /locus_tag="VHV99_22640"
     CDS             1136..2827
                     /locus_tag="VHV99_22640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX3638221.1"
                     /translation="MTAAKGFLEARDLLLRHRTDYERAYREFAWPAPGEFNWALDYFD
                     VIARNNDNPALWIVDDPASDGLRLSYAQMSERSARMANFLRGLGVGRGDKLLLMLPNR
                     VELWDAMLAAMKLGAIVLPATTQLSADDVRDRVQIGGATFVVVDSAELAKFDALDVPL
                     TRISVGAPRDGWIDLAAAYDASPQFTPEGVTHATDPLLLYFTSGTTSKPKLVEHTHQS
                     YPVGHLSTMYWIGLQPNDIHWNISSPGWAKHAWSCFYAPWNAQACVFVFNFPRFVPKD
                     TLNALVRFNVTTLCAPPTVWRMLVQEHLTDYPVKLREIVGAGEPLNPEIIERVKHAWG
                     ITIRDGFGQTETTCQIGNPPGQPVVPGSMGRPLPGYKIELLDADDQPVTEGEISLPLA
                     DRPLGLMTGYANNPNATAQAMRNGFYHTSDVAMRREDGYYVYVGRADDVFKSSDYRLS
                     PFELESVLIEHEAIGEAAVVPSADALRLSVPKAFVTVRQGYEASPELARAVFAFSREK
                     LAPYKRIRRLQFSELPKTISGKIRRVELRRREMEREAEPARLPGEYWEEDFPDLR"
     gene            2887..>3362
                     /locus_tag="VHV99_22645"
     CDS             2887..>3362
                     /locus_tag="VHV99_22645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX3638222.1"
                     /translation="MSAIASNQAATVKLLINGEFVESKTTEWRDIVNPATQEVLARVP
                     FATADEVNEAIRSAHAAFKTWKDTPIGARMRIMLKFQALIREHSPRIAKTLSAEQGKT
                     IPDAEGDIFRGLEVVEHACSIGTLQQGEFAENVAGGVDTYTLRQPIGVCAGITPFN"
BASE COUNT          671 a         1078 c         1022 g          591 t
ORIGIN      
        1 cgcctgcgcc acgctgatga gtgtgcataa ctcggtgggc tgcggcccga tcctgaactt
       61 cggcaccgac gcgcaaaaag accgctatct gcaagacctc gccacgggcc gccgcatcgg
      121 cgcgttctgc ctgaccgagc cgcaagccgg ctccgaggca aacaatttgc gcactcgcgc
      181 ggtgctgcgc gacggcaaat ggattctcaa cggcaacaag cagttcgtga ccaacggcgc
      241 gcgcgccaat atcgcgatcg tgtttgccgt caccgatcct gaccgcggca agcgtggtct
      301 atcggccttc atcgtgccga ccgatacgcc gggcttcaac gtcggcaagc ccgaacacaa
      361 gctcggcatt cgcgcctcgg acacctgccc gatctcgctc gacgattgcg cggtgcccga
      421 agccaatctg ctcggcgaac caggcgaagg cctgcgtatt gcgttgtcga atctcgaggg
      481 tggccgcatt ggtatcgcgg cgcaggcggt cggcatcgcg cgggccgcat tcgacgccgc
      541 acgccgctac gcgaacgaac gcattcaatt cggcaaggcg ctgaaggatc accagaccat
      601 cgccaacatg ctcgccgaca tggcgacccg gctgaacgcc gcgcgcctgc tggtgcatca
      661 cgcggcgcgg ctgcgcacgg cgggcaggcc gtgtttgtcc gaggcctcgc aggccaagct
      721 gtttgcgtcg gaactcgccg aggaaatctg ctcgaacgcg atccagattc atggcggcta
      781 cggctatctt gaggactatg cggtggaacg ccattatcgc gacgcccgta tcacgcagat
      841 ttacgaagga accagtgaag tgcagcggat ggttattgca cgccatgttt aaacgcaggt
      901 tagatggcgt ttgagtgcaa aatagcttgc tagtagtaag aagcagaggc aaaaaacaga
      961 gcgcataccg cgccaccagc tttgcatggg accagagtaa gcgctgaagc gctaactccg
     1021 accgacagct ttgcatggga tcggagtaag cgctgaagcg ctaactccaa tcgacagctt
     1081 tgcatggggt cggagtaagc gctgaagcgc taactccgac cgacaggaga cgacgatgac
     1141 tgcagcaaag ggctttctcg aagcacgcga tctgttattg cgccatcgaa cggactacga
     1201 gcgcgcctac cgcgaattcg cgtggccggc gccgggcgag ttcaactggg cgctcgacta
     1261 cttcgacgtg atcgcgcgaa acaacgacaa tcccgcgctg tggatcgtcg acgatccggc
     1321 cagcgacggc ctgcgcctct cctacgcgca aatgtcggaa cgctcggcgc gcatggcgaa
     1381 cttcctgcgc ggcctcggcg tgggacgcgg cgacaaattg ctgctgatgc tgccgaaccg
     1441 cgtcgaactc tgggacgcga tgctcgcggc catgaagctc ggcgcgatcg tgctgcccgc
     1501 caccacccag ctttccgccg acgacgtgcg cgaccgcgtg cagattggcg gcgcgacgtt
     1561 cgtggtagtc gatagcgcgg agctggccaa gttcgatgca ctcgacgtgc cgctcacccg
     1621 catctccgtc ggcgcgccgc gcgacggctg gatcgacctt gccgcggcct atgacgcatc
     1681 gccacaattt acgcccgaag gcgtcacgca cgccaccgat ccgctgctgc tgtatttcac
     1741 gtcgggcacc acgtccaaac cgaagctggt cgagcatacg caccagagct acccggtcgg
     1801 gcatctgtcc acgatgtact ggatcggcct gcagccgaac gacatccact ggaacatcag
     1861 ttcgccgggc tgggccaagc acgcgtggag ttgcttctac gcaccgtgga acgcgcaggc
     1921 ctgcgtgttc gttttcaact ttccgcgctt cgtgccgaaa gacacgctca acgcgctggt
     1981 gcgtttcaac gtcaccacgc tgtgcgcacc gcccaccgtc tggcgcatgc tggtgcagga
     2041 gcatctcacc gactatccgg tgaagctgcg cgagatcgtc ggcgcgggcg agccgttgaa
     2101 tcccgagatc atcgaacgcg tcaaacatgc gtggggcatc acgattcgcg acggtttcgg
     2161 tcagaccgaa acgacctgcc agatcggcaa cccgccgggg caaccggtgg tgccgggctc
     2221 gatgggccgt ccgctgccgg gctataaaat cgaactcctc gatgccgatg atcaacccgt
     2281 caccgaaggc gaaatttcgc tgccgttggc cgaccgtccg ctcggtttga tgaccggcta
     2341 cgcgaacaat cccaacgcca ccgcgcaagc catgcgcaac ggtttctacc acacctccga
     2401 cgtcgcgatg cgccgtgaag acggctacta cgtctacgtc ggccgcgccg atgacgtttt
     2461 caaatcgtct gattaccgac tgagtccatt cgaactcgaa agcgtgttga tcgaacacga
     2521 ggcgattggc gaagcggccg tggtgccgag tgccgacgcg ttgcgtttgt cggtgcccaa
     2581 agcgttcgtc accgtgcgtc agggttacga agccagcccc gaactcgccc gcgcggtctt
     2641 cgcgttctcg cgcgaaaaac tggcaccgta caagcggatt cgccgcctgc aattcagcga
     2701 gctgccgaaa accatctccg gcaagattcg ccgcgtcgaa ttgcggcgcc gcgaaatgga
     2761 gcgcgaggcc gagcccgcgc gcctgcccgg cgagtattgg gaagaagatt ttccggacct
     2821 gcgttgatta tttttatttc ccgtcactgt tcggccgcga agcggccact gcacaggagt
     2881 tccagcatga gcgcaattgc ttcgaaccag gccgccaccg tcaaactgtt gatcaacggc
     2941 gagttcgtcg aatccaaaac caccgagtgg cgcgacatcg tcaacccggc tacgcaggaa
     3001 gtgctggcgc gcgtaccgtt cgccaccgcc gatgaagtga acgaagcgat ccgctccgcg
     3061 cacgccgcgt tcaagacctg gaaggacacg ccgatcggcg cgcgcatgcg catcatgctg
     3121 aagttccagg cactgattcg cgagcattcg ccgcgcatcg cgaagaccct cagcgccgag
     3181 cagggcaaga cgattcccga tgcggaaggc gacatcttcc gcgggctgga agtggtcgag
     3241 catgcctgct cgattggcac cttgcaacag ggtgaattcg cggaaaacgt ggcgggcggt
     3301 gtggatacgt acacgttgcg tcaaccgatc ggcgtatgcg ccggcatcac gccgttcaat
     3361 tt
//
LOCUS       DATASH010000847         7264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3110428,
            whole genome shotgun sequence.
ACCESSION   DATASH010000847 DATASH010000000
VERSION     DATASH010000847.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7264
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3110428"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VHV99_22650"
     CDS             <1..210
                     /locus_tag="VHV99_22650"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010101549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEX3638223.1"
                     /translation="FPFIMPSSLDGRSSLTVWDSTSSRMTLQIMLIAVIIFLPIVLIY
                     TSWVYRVMRGKVTAAALEENHHSMY"
     gene            239..355
                     /gene="cydX"
                     /locus_tag="VHV99_22655"
     CDS             239..355
                     /gene="cydX"
                     /locus_tag="VHV99_22655"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HEX3638224.1"
                     /translation="MWYFSWFLGIGVALAFGIINVMWLESRRPVEAGKQKTR"
     gene            complement(492..1814)
                     /locus_tag="VHV99_22660"
     CDS             complement(492..1814)
                     /locus_tag="VHV99_22660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1996 domain-containing protein"
                     /protein_id="HEX3638225.1"
                     /translation="MSMRSAICLMLFLPAGAAYAGGQFNVQCAYSHTLPDDAIVYPGQ
                     PGRAMVHDFFGNTGADAYSTYYSLNNDKLTTCNVAADLSSYWVPQLNRASGIVVPNYQ
                     KTYYKNDQPVVPLHTIPAGLEMLAGDHHSSVPKPQINYLCRGGSYTQIAPTNCPVVTD
                     DNGTYAQLNISVHFPDCWDGRTLVPNMANHIMNMAYRQSDGKCPAAYTVKIPELQLNV
                     AYDLGQDPDLSTAQLSMDPLLVNGSWVPQWGSLYTAHGDFINAWKTDSLQYAVDNCSN
                     QNIACNNSIPTYYSKASADVWMDGGGVVHASDATLTLDASGVVLVKFPTPTDLNAYPY
                     TNSYLQTLAQNVTDTDAVMLDLYAATTNWDDVATLPTAAACDASKRIGGIYLDNALQP
                     RINDITGYVASQVSAGAPQIGVCIRNATGRTIQISSRDGARTPALFMK"
     gene            complement(2071..3858)
                     /gene="nagE"
                     /locus_tag="VHV99_22665"
     CDS             complement(2071..3858)
                     /gene="nagE"
                     /locus_tag="VHV99_22665"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HEX3638226.1"
                     /translation="MDGNPFLKIQRLGRALMLPIAVLPVAGLLLRLGQPDVFNIKMIA
                     DAGGAIFDNLPLLFAIGVAVGFAKDNNGVAGLAGAIGYLVEIAVMKDINDKLNMGVLS
                     GIVAGIVAGLLYNRYKDIKLPDYLAFFGGKRFVPIVTGVVCLAIGIVFGYVWQPVQAV
                     IDTAGHWLTTAGALGAFVFGVLNRLLLVTGLHHILNSLTWFVFGSFTPPGGAAVTGDL
                     HRFFAGDPTAGTFMTGFFPVMMFGLPAACLAMYHEAPKERRAMVFGLLASMALTSFLT
                     GVTEPIEFSFMFLAPVLYAIHAVLTGLSLAICSALGIHLGFTFSAGFIDYVLNYGLST
                     KGWWAIPIGLVYMVVYYGLFRFFIRKFNMATPGREPAAEEEQVESFTAGGFVSPVAGA
                     AVPRAQRYIAALGGASNLSVVDACTTRLRLSVVDSSKVSESELKTIGARGVLKRGSTN
                     VQVIIGPEADIIADEIRTVIAQGGGEAVKDVAKPAAAAPAVAAAPVAAASGAQAGPLD
                     PDPLRWLAVFGGAGNVVSLDAVAATRLRIVVRDPSAVDRQRLATLDTAWVSTDTFHIV
                     VGDAAQRYAAQLATRLSPDTGAGTVPQPA"
     gene            complement(3956..6520)
                     /gene="ptsP"
                     /locus_tag="VHV99_22670"
     CDS             complement(3956..6520)
                     /gene="ptsP"
                     /locus_tag="VHV99_22670"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEX3638227.1"
                     /translation="MSHSEGHIVLLAPMTGPVVPLANVPDPVFSGGMFGDGMGIDPLE
                     GRLVAPCDGTITHLARTGHAVTLATAEGVEILLHIGIDTVELNGKGFAPMVAQGAHVR
                     AGDVLIEFDQDQVALNAPSLVSVIAIANSDAFEIVERVQDGMLKAGETPLLVLRARGG
                     EAAHASRQASATNVTEEARQQVTLVHAGGLHARPAARAREAARGFDARVEVRYEGRKA
                     AIESVVGLLGLGAGEGATVELLGVGPQAQAAIDAIAHELTREAHGEVEEKPARQSSPA
                     PQAVVHAAGETLAPNTLAGVCASPGVAVGKLVRWDDADIDPPEKANGTSAAESRLLDK
                     AIATVDADLGTTVRDASQRGAVGEAGIFAVHRVLLEDPTLVDAARDLISLGKSAGFAW
                     REAIRAQIAILTNIEDALLAERAADLRDIEKRVLRTLGYTNAAARTLPEEAVLAAEEF
                     TPSDLSTLDRSRVTALVMARGGATSHAAILARQAGIPALVAVGDALHAIPEGTQVVVN
                     ATTGRLEFAPTELDLERARLERSRLAGVREANRRTSQQAAATSDGRAIEVAANIATLD
                     DAKTAVDNGADSVGLLRTELLFIHRAAAPTIDEHRQSYQAIVDALSGRTAIIRTLDVG
                     ADKEVDYLTLPPEPNPALGLRGIRLAQVRPDLLDDQLRGLLAVQPLGAVRILLPMVTD
                     VGELIRIRNRIDEFARELGRTEPIEVGVMIEVPSAALLADQLSQHADFLSIGTNDLTQ
                     YTLAMDRCQADLAAQADGLHPAVLRLVAATVQGADKYGKWVGVCGALAGDPLAVPLLV
                     GLGVTELSVDPVSVPGIKARVRNLDYQLCRQRAQDALALESAQAVRAASRETWPLD"
     gene            complement(6559..>7264)
                     /locus_tag="VHV99_22675"
     CDS             complement(6559..>7264)
                     /locus_tag="VHV99_22675"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006024455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX3638228.1"
                     /translation="MEALRKAGALTVAAVNAPNSPLADACEFHLPLIAGPELSVAATK
                     SYIAMLSISALLVAHWQKDDALLGALNTLPGALQAAGKLDWSKAVDELRGIERMIVIG
                     RGLGLAIAQEAALKLKETSGIQAEAFSSAEVRHGPMELIDRDYPLLVFAPRGPEQAGL
                     LQLARDMQARGARVLLAAPADVPEATLPLATTAHAALDPIAAILSFYVMAAGLASARG
                     RNPDAPRHLNKVTETH"
BASE COUNT         1385 a         2320 c         2239 g         1320 t
ORIGIN      
        1 ttcccgttca tcatgccctc ctcgctcgac ggccgcagca gcctgaccgt ctgggattcg
       61 acctcgagcc gcatgacgct gcagatcatg ctgatcgccg tgatcatttt cctgccgatc
      121 gtcctgatct acacgagctg ggtgtatcgc gtaatgcgag gcaaggtgac cgccgcggca
      181 ctcgaagaaa atcatcattc gatgtattga ccggcaatac gattaacagg agtttgtgat
      241 gtggtatttc agttggtttc tcggcattgg cgtggcgctg gcattcggca ttatcaacgt
      301 gatgtggctg gaatcgcgcc ggcctgttga agcaggtaag cagaaaacac gttgatgcat
      361 ccgttgccgg ccgcccggcg ttgggccgaa gccggtaaac gccaaagcgg caccttgaat
      421 caaggtgccg cttttttgtt gacgagagac tcgaccggat gcggcgcttt cccgcttgcc
      481 agcgccgcgc gttacttcat aaatagcgcc ggtgttcgcg caccgtcgcg cgaagaaatc
      541 tgaatggtcc gtcccgtcgc gttgcgaatg caaacgccaa tttgcggcgc gcccgcggac
      601 acttgcgatg cgacatatcc cgtgatgtcg ttgatccgcg gttgcaacgc gttatccagg
      661 tagatgccgc caattcgctt gctcgcgtcg caggctgcag cggttggcag ggtcgcgaca
      721 tcgtcccagt tggtggtggc agcatagaga tccagcatca ctgcatcggt atccgtgacg
      781 ttctgggcca acgtctgcaa gtacgagttg gtgtacggat acgcattcag gtcggtgggc
      841 gtcggaaatt tgaccaggac gacgccgctc gcgtctaagg ttagcgtcgc atcgctcgca
      901 tgcacgacgc cgccgccgtc catccacaca tcagcgctcg ccttcgagta atacgtggga
      961 atgctgttgt tgcacgcgat attctgattg gaacaattgt cgacagcgta ctggagcgaa
     1021 tctgtcttcc aggcgttgat gaaatcgccg tgggccgtat acagacttcc ccattgcggt
     1081 acccacgagc cgttcacgag aagggggtcc atcgacaatt gcgcggtcga gagatcggga
     1141 tcctgcccca ggtcgtacgc aacgttgagt tgcagttccg gaatcttgac ggtataagcg
     1201 gccggacatt ttccgtcgga ctgccggtag gccatattca tgatgtgatt agccatgttc
     1261 ggcaccagag tcctgccgtc ccaacaatcc ggaaaatgga ccgagatatt aagctgcgcg
     1321 tacgtgccgt tgtcgtccgt cacgacaggg cagttggtcg gcgcgatttg cgtgtaggag
     1381 ccgccgcggc atagatagtt gatctgcggc ttgggcacgg acgaatggtg atcgcccgcc
     1441 agcatttcca gccccgcggg aatcgtgtgc agcggaacaa ccggctgatc gttcttgtag
     1501 tacgtcttct gatagttcgg cacgacgatc cccgaggcgc ggtttaactg cggcacccag
     1561 tacgatgaaa gatccgccgc gacattgcag gtcgtcagtt tgtcgttatt cagggagtag
     1621 tacgtgctgt atgcatcagc gcctgtgttg ccgaaaaagt cgtgcaccat ggcccggcct
     1681 ggctgtcctg gatagacgat cgcatcgtcc ggcaacgtat gcgaatacgc acattgaaca
     1741 ttgaactgac cacccgcgta ggcggcgcca gccggcaaga acagcatgag gcagattgct
     1801 gatcgcattg acatgagcaa ctcctgggcg cgaacgaaaa cgccttgccg atatgacaag
     1861 gcgctcgtat ctcgttaatt agcttaccca atcgttttca atacagacca tcgttacgca
     1921 taacattcgc cgccgcgctg ctgcgtcaca ccgctacgca tctaccgtgc atgaaaaaag
     1981 cccccggaaa tcccggaggc tttttgaata acgtggctga cgaacagagc ggaagactga
     2041 agtcacccgc agctcgaagt ctcgatgaga ttacgcgggc tgtggcaccg tacctgcgcc
     2101 cgtatccggc gacagacgcg tcgccagttg tgcggcgtag cgctgcgcgg cgtcgcccac
     2161 tacgatgtgg aacgtatccg tcgagaccca cgccgtatcc agcgtggcga gacgttgacg
     2221 atccaccgcc gacggatcgc gaaccacgat acgcaggcgc gttgccgcca cggcatccag
     2281 cgacacgacg ttgccggcgc cgccgaacac ggccagccaa cgcaacggat ccgggtcgag
     2341 cggaccggct tgagcaccgc ttgccgccgc gacgggcgca gccgcgacag cgggtgcggc
     2401 agcagccggc ttcgcgacat ctttcacagc ctctccacca ccttgcgcga tcacggtacg
     2461 gatttcatcg gcgatgatgt ccgcttccgg tccgatgatc acctgcacgt tggtcgagcc
     2521 gcgcttgagc acaccacgcg cgccgatcgt cttcagttcg ctttccgaga ccttgctgga
     2581 atcgaccacc gacagacgca gacgcgtcgt gcaggcatcc accaccgaca ggttcgacgc
     2641 gccacccagc gcagcgatat aacgctgtgc acgcggcacg gcggcacccg ccaccggcga
     2701 cacaaaaccg ccagccgtga acgactcgac ctgttcttcc tcggcggcgg gttcgcggcc
     2761 cggcgtcgcc atattgaact tgcgaatgaa gaagcggaac aggccgtagt acacgaccat
     2821 gtacacgaga ccgatcggaa tcgcccacca gcctttggtc gacaggccgt agttcagcac
     2881 gtagtcgatg aagccggccg agaaggtgaa gccgagatga atcccaagcg cggagcagat
     2941 cgccagcgac aagccggtca gcaccgcgtg gattgcatag agcaccggcg cgaggaacat
     3001 gaagctgaat tcgatcggct cggtcacgcc ggtcaggaac gaggtgagcg ccatcgacgc
     3061 caacaggccg aacaccatcg cgcggcgctc tttcggagcc tcgtgataca tcgcgagaca
     3121 cgcggccggc aggccgaaca tcatgaccgg gaagaagccc gtcatgaacg tgcccgcggt
     3181 cgggtctccc gcgaagaaac ggtgcaggtc gcccgtgacc gcggcgccgc ccggcggcgt
     3241 gaaggaaccg aacacgaacc acgtcagcga attcaggatg tgatgcagcc ccgtgacgag
     3301 cagcaaacgg ttcagcacgc cgaacacgaa tgcgccgagc gcgcccgcag tggtgagcca
     3361 atggccggcc gtatcgatga cagcctgcac cggctgccac acgtagccga acacgatgcc
     3421 tatcgccaga cagaccacgc cggtgacaat cggcacgaag cgtttccctc cgaagaatgc
     3481 gaggtagtcg ggcaacttga tgtccttgta cctgttgtac agcaagcccg cgacgatacc
     3541 cgccacgatc ccggacaaca cgcccatgtt gagcttgtcg ttgatgtcct tcatcaccgc
     3601 gatttcgacc agataaccga tcgcacccgc gagacccgcc acgccgttgt tgtctttcgc
     3661 gaagccgacc gccacgccga tcgcgaacag caacggcagg ttgtcgaaga tcgcgccgcc
     3721 ggcgtcggcg atcatcttga tgttgaacac atcgggctgg cccaggcgca gcagcaggcc
     3781 ggcaaccggc agcactgcaa tcggcagcat cagcgcgcgt cccaggcgct gaatcttcag
     3841 aaacggattc ccatccattg acacctccaa tccttgtctc gtggtcgact gtcgtcgttg
     3901 agttttgtag ctatacgaag taattgagcg aagctggttg cggaccgtgt ggggttcagt
     3961 ccaatggcca ggtttcgcgg cttgctgctc ttaccgcctg cgccgattcg agcgccaggg
     4021 catcctgggc gcgttgacgg cacagctgat aatcgaggtt gcgcactcgc gccttgatgc
     4081 ccggcaccga caccggatcc accgagagct cggtcacgcc aaggccgacg agcagcggca
     4141 cggcaagcgg atcgcccgcc aatgcgccgc atacaccgac ccatttgcca tacttgtccg
     4201 cgccttgcac cgtcgctgcg accaggcgca gcactgcggg atgcaagccg tccgcttgcg
     4261 ccgcgaggtc ggcctggcaa cggtccatgg cgagcgtgta ttgcgtcaga tcgttggtgc
     4321 cgatcgaaag gaaatccgcg tgttgtgaca gctgatcggc gagcaacgcg gccgacggca
     4381 cttcgatcat cacaccgact tcgatcggct cggtacgacc caattcgcgg gcgaattcgt
     4441 cgatgcgatt gcggatgcgg atcaattcgc cgacgtccgt gaccatcggc agcaggatgc
     4501 gtacggcgcc gagcggttgt accgccagca ggccgcgcaa ctgatcgtcg agcagatccg
     4561 ggcgcacttg tgcgaggcgg atgccgcgca ggccgagcgc cggattcggt tcgggtggca
     4621 gcgtcaggta atcgacttcc ttgtccgcgc cgacgtccag cgtgcggatg attgcggtgc
     4681 ggccgctcaa tgcatcgacg atcgcctggt agctctgacg atgttcatcg atggtcggcg
     4741 ccgcggcgcg gtgaatgaac agcagttcgg tgcgcaacag gccgacggaa tcggcgccgt
     4801 tgtcgaccgc ggttttggcg tcgtcgaggg tggcgatgtt cgcggcgact tcgatcgcgc
     4861 ggccatcgga cgtcgctgcc gcctgttgcg acgtgcggcg gttcgcttcg cgcacgccgg
     4921 ccaggcgact gcgttcaagg cgcgcacgtt cgagatcgag ctcggtcggc gcgaattcga
     4981 ggcggccggt ggtggcgttc accaccactt gcgtgccttc ggggatcgcg tgcaaggcat
     5041 cgccgactgc caccagcgcc ggaataccgg cctggcgcgc gagaatcgcc gcatgcgaag
     5101 tcgcgccgcc acgcgccatc acgagtgcag tgacgcgcga gcgatcgagt gtggacaaat
     5161 ctgacggcgt gaactcctcc gccgccaaca cggcttcttc cggcagcgtg cgcgccgcgg
     5221 cattcgtata gccgagcgta cgcaacacgc gcttttcgat gtcgcgcaga tcggcggcac
     5281 gttcggcgag caaggcgtct tcgatattcg tcaggatggc gatctgtgcg cggatcgctt
     5341 cgcgccacgc aaacccggcg ctcttgccga gactgatcag atcgcgcgcg gcgtcgacca
     5401 gcgtggggtc ttccagcagt acgcgatgca ccgcgaaaat gcccgcctcg cccaccgcac
     5461 cgcgttgcga cgcatcgcgc acggtggttc cgaggtcggc gtcgacagtg gcaatcgcct
     5521 tgtcgagcag acggctttcc gccgccgacg tgccattcgc cttttccggc ggatcgatgt
     5581 ccgcatcgtc ccagcgcacc agcttgccga ccgcgacgcc cggcgacgcg cacacacctg
     5641 cgagcgtatt cggcgcgagc gtttcgcccg ctgcgtgtac aaccgcttgc ggcgcgggtg
     5701 agctttgacg tgccggcttt tcttcgactt cgccgtgtgc ttcacgcgtc aattcatggg
     5761 caatcgcatc gatcgcggct tgagcttgcg ggccgacgcc gagcaactcg acagtcgcgc
     5821 cctctccggc accgagaccg agcaaaccaa ccacgctctc gatcgccgcc ttgcgccctt
     5881 cataacgcac ttcgacgcgc gcatcgaaac cgcgcgccgc ttcacgagcg cgcgcggccg
     5941 gacgtgcatg caaaccgcct gcgtgaacca gcgtgacttg ctgacgggcc tcttcggtca
     6001 cgttagtagc gctcgcctgg cgcgacgcgt gcgccgcctc gccgccgcga gcgcgcagca
     6061 ccagcaacgg cgtttcgcct gccttcagca tgccgtcctg cacacgctcg acaatctcga
     6121 acgcgtccga attggcaatc gcgatcaccg aaacgagact cggcgcgttc agcgcgacct
     6181 ggtcctgatc gaactcgatc aggacgtcgc cggcacgcac atgggcgcct tgcgcgacca
     6241 tcggcgcaaa gcccttgccg ttcagctcga ccgtatcgat gccgatgtgc agcagaatct
     6301 ccacaccttc ggccgtcgcg agcgtgaccg cgtgaccggt gcgcgcgaga tgggtgatgg
     6361 tgccgtcgca cggcgcgacg aggcgcccct cgagcggatc gatacccatg ccgtcgccga
     6421 acatgccgcc cgaaaacaca gggtcgggca cattcgccag cggcacgacc ggtccggtca
     6481 tcggcgcgag caaaacaata tggccttcag aatggctcat caagcgggac tcctcgacac
     6541 gtcgcatctg atttctcgtc agtgagtttc ggtgactttg ttgagatggc gtggcgcgtc
     6601 aggattgcgc ccgcgtgcgg aggcaaggcc ggcggccatc acgtaaaagg acaggatcgc
     6661 ggcaatcgga tcgagcgccg catgagcggt ggtggcgagc ggcagcgtcg cttccggcac
     6721 atcggccggt gcggcgagca gcacgcgggc gccgcgcgcc tgcatgtcgc gcgcgagttg
     6781 cagcaaaccg gcctgctccg ggccgcgcgg tgcaaagacc agcagcggat agtcgcggtc
     6841 gatcagctcc atcggaccgt gccgcacttc cgcgcttgaa aacgcttcgg cctggatgcc
     6901 ggaggtttcc ttcaatttca gcgcggcttc ctgcgcgatt gcaagaccca gaccgcggcc
     6961 gatcacgatc atgcgctcga tgccacgcag ttcatccacc gctttcgacc agtcgagctt
     7021 gcctgcggct tgcagcgcgc cgggcagcgt gttcaacgcg ccgagcaacg catcgtcttt
     7081 ctgccaatgc gcgaccagta gcgcggaaat cgacagcatc gcgatgtagc tcttggtggc
     7141 cgcgacactc agttccggac cggcaatgag cggcagatga aattcgcaag cgtcggccag
     7201 cggcgaattg ggcgcattca ctgcggccac cgtcaatgcg ccggccttgc gcaacgcttc
     7261 catc
//
LOCUS       DATASH010000848         2814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3902279,
            whole genome shotgun sequence.
ACCESSION   DATASH010000848 DATASH010000000
VERSION     DATASH010000848.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3902279"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VHV99_22680"
     CDS             <1..314
                     /locus_tag="VHV99_22680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638229.1"
                     /translation="VFQDLTLGRHDLADPVHGEDVLGHLLAIDVDDAGRVGAGGRGWM
                     GSRCYGRAVRPTPAGAAPSGAWNFATPRARAGGSGGIVLRGGGGAAEGVGSRTFNRFE
                     A"
     gene            complement(358..1446)
                     /locus_tag="VHV99_22685"
     CDS             complement(358..1446)
                     /locus_tag="VHV99_22685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
                     /protein_id="HEX3638230.1"
                     /translation="MAALTLQGVKKTYDGKQFVLHGIDVDVADGEFVVMVGPSGCGKS
                     TLLRMVAGLEGISEGTISIAGKVVNKLEPKDRNIAMVFQNYALYPHMSVAENMGYALK
                     IAGVDRAQIVKRVQAAAQILELEPLLARKPRELSGGQRQRVAMGRAIVREPAVFLFDE
                     PLSNLDARLRVQMRLEIQRLHARLATTSLYVTHDQIEAMTLAQRVIVMNKGHAEQIGA
                     PTDVYERPATVFVASFIGSPGMNLLEGRVSDDGSMFEVAGNGPKLPLTGVAAIGREVA
                     RGREWTLGIRPEHMTPGQADAAHATLTVDSCELLGADNLAHGRWGKHDVTVRLPHTHR
                     PAAGETLQVALPAQHLHFFDPSTGRRAN"
     gene            complement(1538..2383)
                     /gene="ugpE"
                     /locus_tag="VHV99_22690"
     CDS             complement(1538..2383)
                     /gene="ugpE"
                     /locus_tag="VHV99_22690"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HEX3638231.1"
                     /translation="MIENRRGFDLFCHAVLILGVVLVVFPVYVAFCAATMSEHEVFSV
                     PLSLVPSTHLFENIATVWSQGSGNAAAPFGRMLFNSLVMALVISIGKIAVSMISAYAI
                     VFFRFPFRNLAFWLIFITLMLPVEVRIFPTVQVVSSMHLSNTYSGLTLPLIASATATF
                     LFRQFFMTLPDELMEAARIDGAGAMRFFWDVVLPLSKTNMAALFVITFIYGWNQYLWP
                     ILITSQQSLTTAVVGIKSMIASGDTATEWHLVMTATLLAMLPPLAVVLTMQRWFVRGL
                     VDAEK"
     gene            complement(2380..>2814)
                     /locus_tag="VHV99_22695"
     CDS             complement(2380..>2814)
                     /locus_tag="VHV99_22695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX3638232.1"
                     /translation="LNAGQAMFLVVLASVWKQVSYNFLFFYAGLQAIPRSLIEAAAID
                     GAGPVRRFFGIALPLLSPTSFFLLVINITYAFFDTFPVIDAATGGGPAQATRTLIYKI
                     FAEGFQGLDIGSSGAQSVILMVIVVALTVVQFRFIERRVQYS"
BASE COUNT          547 a          870 c          890 g          507 t
ORIGIN      
        1 atgtctttca ggatttgacg cttgggcggc acgatcttgc cgacccggtt catggtgaag
       61 acgtattggg ccatttgctt gcaatagacg ttgacgacgc cggccgcgtg ggggcgggcg
      121 gtcgtgggtg gatgggtagt cggtgttatg gccgggcagt gcgcccgacg ccggccggcg
      181 cggcgccgag cggggcgtgg aacttcgcga cgccccgcgc gcgggcgggt ggttcaggtg
      241 gcattgtact tcggggcggg ggtggggcgg cagagggtgt cggttcacgc accttcaacc
      301 ggttcgaggc ttaaagccaa tctgccacgc cgccgtgccg cgagcaggtg ccgctgctca
      361 gttcgcccgc cgtcccgtcg acggatcgaa gaaatgcaga tgttgcgcgg gcaacgcgac
      421 ttgcaacgtt tcacctgccg cgggccgatg cgtgtgcggc aagcggaccg tgacgtcgtg
      481 cttgccccag cggccatgcg cgaggttgtc cgcgccgagc agttcgcagg agtcgacggt
      541 cagcgtggcg tgcgcggcgt ccgcctggcc cggcgtcatg tgctcagggc gaatgccgag
      601 cgtccattcg cggcccctgg caacttcccg gccgattgcc gccacgccgg tgagcggcag
      661 cttcggtcca ttgcctgcga cctcgaacat ggaaccgtcg tccgacacac ggccttccag
      721 cagattcatc cccggcgagc cgatgaagct cgcgacgaac acggtcgccg gtcgttcgta
      781 gacatcggtc ggcgcgccga tctgctccgc gtgccccttg ttcatcacga tcacgcgttg
      841 cgccagcgtc atcgcttcga tctggtcgtg ggtgacataa aggctagtcg tggcaagccg
      901 cgcgtgcagg cgctggattt cgaggcgcat ttgcacgcgc agccgtgcat cgagattgga
      961 cagcggttcg tcgaacagaa acacggccgg ttcgcgcacg atcgcgcggc ccattgcaac
     1021 gcgttgccgc tgaccaccgg acaactcgcg cggcttgcgt gcgagcagcg gttcaagctc
     1081 gagaatctgc gccgcagcct gcacgcgctt cacaatctgc gcgcgatcga cgcccgcgat
     1141 cttcagcgcg tagcccatgt tctcggccac gctcatatgc ggatacagcg cgtagttctg
     1201 gaacaccatc gcgatgttgc gatccttcgg ctccagtttg ttgaccactt tgccggcgat
     1261 cgagatggtg ccttcggaaa tgccttcgag cccggccacc atccgtagca acgtcgattt
     1321 gccacaaccc gacgggccca ccattacaac gaactcgccg tccgcgacgt ccacgtcgat
     1381 gccgtgcaac acgaactgtt tgccgtcgta ggttttctta acgccttgca gagtcagtgc
     1441 agccatgccg ttttaacctt ttcgttgctt gccgcgcgag ccacgcttgg cgcgggatgt
     1501 tcttttttat tccaggagca cgcccaaacg gtgccgctca cttctctgca tccaccagtc
     1561 cgcgcacgaa ccagcgctgc atcgtcagca cgacggcgag cggcggcaac atggcgagca
     1621 gcgtcgcggt catcacgaga tgccattcgg tggccgtgtc gcccgaggcg atcatgcttt
     1681 tgatgccgac cacggccgtg gtcaacgatt gctggctcgt aatcaggatc ggccacaggt
     1741 actgattcca gccgtaaatg aaggtgatca cgaacagcgc cgccatattc gttttcgaca
     1801 gcggcagcac cacgtcccag aagaaacgca tcgccccggc gccgtcgatg cgcgccgctt
     1861 ccatcaactc gtcgggcagc gtcatgaaga actggcggaa caggaaggtg gcggtggccg
     1921 aggcgattag gggcaacgtc aacccactgt aggtattgct cagatgcatc gacgacacga
     1981 cctgcacggt cgggaagatc cgcacttcga ccggcagcat cagcgtgatg aagatcagcc
     2041 agaacgcgag attgcgaaac ggaaaacgga agaacacgat cgcgtacgcg gagatcatcg
     2101 agacggcgat cttgccgatc gaaatcacca gcgccatcac gaggctgttg aacagcatgc
     2161 ggccgaacgg tgcggctgca ttgccgctgc cttgggacca cacggtggcg atgttttcga
     2221 acagatgcgt gctcggcacc agcgacaacg gcacgctgaa cacctcatgc tcgctcatcg
     2281 tggccgcgca gaacgcgaca tacaccggaa acaccaccag cacgacgccg agtatcagca
     2341 ccgcatggca aaagaggtcg aaaccgcgtc ggttctcgat catgagtatt gaaccctgcg
     2401 ttcgatgaag cggaattgca cgacagtcaa cgcgacgacg atcaccatca ggatgacgga
     2461 ctgcgcaccc gagctgccga tgtcgagtcc ctggaaacct tcggcgaaaa tcttgtagat
     2521 cagcgtgcgg gtggcttgcg cggggccgcc gcctgtcgcc gcgtcgatca cggggaaggt
     2581 gtcgaaaaac gcgtaggtga tgttgatcac cagcaggaag aagctggtcg gcgagagcag
     2641 tggcagggca atgccgaaga aacgccgcac gggaccggcg ccgtcgatgg ctgctgcttc
     2701 gatcagtgag cgcggaatcg cctgcaagcc ggcatagaag aacagaaagt tatagctgac
     2761 ctgtttccac acggatgcca gcacgaccag aaacatcgcc tggcccgcat tcag
//
LOCUS       DATASH010000849         8740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2912056,
            whole genome shotgun sequence.
ACCESSION   DATASH010000849 DATASH010000000
VERSION     DATASH010000849.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 8740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8740
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2912056"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="VHV99_22700"
     CDS             complement(<1..732)
                     /locus_tag="VHV99_22700"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3638233.1"
                     /translation="MSETLIVAGWIVKGVADRHTAEVIRDGALYQRDGVIVEIGGAQD
                     LVAKYPNAPRLGSPDTVLMPGFVNSHHHVGLTPLQLGSPDHALELWFASRMGTREIDL
                     YLDTLYSAFEMIASGITTVQHIHGWRPGPLSSIHEAASGVLKAYQDIGMRASYSFAVR
                     EQNLLVYESDEDFVKRLPADIGAKLAAHLKAQRVPLADHLQLFDMLTQDNEGQSLTRI
                     QLAPANLHWCSDETLLAIKERADKAG"
     gene            complement(762..1643)
                     /locus_tag="VHV99_22705"
     CDS             complement(762..1643)
                     /locus_tag="VHV99_22705"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX3638234.1"
                     /translation="MNRLATLSTLQVAALQLPPCTSDLASNEAVAFAAVHAAADDGAQ
                     LIVLPELSMVPYFASAPGGQYRDWAEPADGPLAARCAALARTRGIALFVPFYEIDRTT
                     GQYHNAVLGFDAGGNPLRAGPVARKLHLPVGDDPPPGYDEAAHFTPGDALHVVQVGAW
                     RIGVLVCYDRRFPECWRALRAAGADLVIVPVAGSGGDDMDFFVGELRTHARENGLAVV
                     CANKIGDEFLDGVRIDNYGESCVIAADGAVLTRRPGNEGPGIVSATLAHAQIAATRER
                     LSYFDHRRVDLYDTPSF"
     gene            complement(1640..2452)
                     /locus_tag="VHV99_22710"
     CDS             complement(1640..2452)
                     /locus_tag="VHV99_22710"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3638235.1"
                     /translation="MSQHKSASVQPPLVTCMGVGKTWQTDGAPFVALRDIELDIARRE
                     FVVFLGPSGCGKSTLLYLISGLEQVSSGRIMCGGAEVSGPGTDRGLVFQDASLFPWLS
                     VGENITFGLKMQHMPKENRRRIAEEILERVGLSEAIDKRPDQLSGGMRQRVAVGRALA
                     LKPEILLLDEPFAALDVQTRAKMQDFLIQVWQRSNVSMVFVTHHIDEAIALADRIVVF
                     TARPGRIKTIIPVDLPRPRDTRSRAFHDLSVHLTDLMRDEVDRAFAEQERVR"
     gene            complement(2454..3353)
                     /locus_tag="VHV99_22715"
     CDS             complement(2454..3353)
                     /locus_tag="VHV99_22715"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3638236.1"
                     /translation="MKADPTTLFAHRRVVRPAASVRPRSNLVRRLVPLGWGVASLTCF
                     IAAWELAWAAGWADPLLLPPPHIFLRNFASQAQYFIPTDVIGEASNATALKSLAMTIV
                     ASTLRVLSGLVLGFVCSVALGVAIRYFRLFGNLTLPTITLLAPISPIAWLPVAIFLFG
                     IGNAPAVFMVFISLFFVMTVSTISQIDNVSRTHLNVAAVMGATRAQTFRHVILPAILP
                     SLFMVLRMNLFGAWMVVLVAEATGVGSGLGQVVMLARNTFNSSLSFFTMTLIGVIGFA
                     FDVALRIVQRKVLFWVPNNGLGK"
     gene            complement(3350..4411)
                     /locus_tag="VHV99_22720"
     CDS             complement(3350..4411)
                     /locus_tag="VHV99_22720"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3638237.1"
                     /translation="MFRKFVQQDPNGYPLTELGSTRREFLMRGACLAGAAALGGAYLP
                     QAMAAGPLTLKATHGTGLCNCPFFLVKERNLAQGVSLDFVTTPSNADIAALFGAGVVD
                     VSVVPYTNFMTLYDAGAPIKIVAGSGVQGCVIVAQPGITSAAQLRGKTIGTFQADTLE
                     MLPYDYLKKAGMSFADVKVRYFGTSPELAQAFIAGNVDAMCHVEPYATQALKARPGSV
                     QLSNGVDVYGANYSDCVLAVRSKLLHENRDAVKALIKAMMTAQHQEELDRASAVKDTV
                     GKYFKTSYDATLDAAGKQPAMVDQRSNQNFILQRAQNLKDLRYIKRLPGPDMFDWGPL
                     NEVIKENADLYGQLKLKSA"
     gene            complement(4422..5339)
                     /locus_tag="VHV99_22725"
     CDS             complement(4422..5339)
                     /locus_tag="VHV99_22725"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3638238.1"
                     /translation="MQKLVLAHGSALSNLALFVAVDSGLFERHGLRAEAPPLTHFGST
                     AALLRSGEADLGTTGFTQPLVDHAQPDPLRIVGGSGKLGMALMGQPGLAMSDLRGQRV
                     GTFADDPMEVLLHDALRVHGMDSMQIERVLYPSLAEAVGDLLAGRLAALTLIEPWISR
                     LRPSGFEVLCDGLHAWGGDYPDTLLVARKSFLDAHPELIRAALRAMLDAQEAITNTPR
                     EALRASAHRYPEFTLDELLAGIAAQPPSVDIRPLRACILARARSLEALGRMQISGSIG
                     EIFSFDLLEQTLAQSRAETFISPSAVQPL"
     gene            5815..6546
                     /locus_tag="VHV99_22730"
     CDS             5815..6546
                     /locus_tag="VHV99_22730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX3638239.1"
                     /translation="MATDKKRARTAENEVAGSFDGAHRHVIYERLMNAISEHRLQPGT
                     KLSEDRLASIFNVSRTTVRSVLQQLSHELVVTIVPNRGAFVASPTVEEALELFEARRQ
                     IELPIIEKVCRCATKKDLARLRAHVAQEDEARKRDDRRTTVRLSGEFHVMLAQVSGNR
                     FIERTMRGLQMLTCLTILLYNTPTAQACLHDEHARLIDAVEAGNVKLAQKIMDEHLEH
                     IEATLRLQGPEEESDDLEAILVHGL"
     gene            complement(6559..7755)
                     /gene="argE"
                     /locus_tag="VHV99_22735"
     CDS             complement(6559..7755)
                     /gene="argE"
                     /locus_tag="VHV99_22735"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008777 - acetylornithine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HEX3638240.1"
                     /translation="MFDKPTLDTNERHAYLALTEELIRYDTTSSRSNLGLIEAIRDRL
                     RAHHIETLLVYDRARQKANLFATLPSFNGETNGGVVLSGHTDVVPVTGQNWSSNPFDP
                     VIRGDRLYGRGSCDMKGFIAVTLGLLPDIQKRRLREPVHFAFSFDEEVGCLGAPLLLA
                     ELEKRGIKPAGCIVGEPTSMRPVVAHKGNNAYRCCVRGVAAHSSRTPSGVNAIEYAAR
                     LICRIRDTADALRRNGPFDRAFDVPYATAQTGLIKGGNAINTIPDDCEFFFEYRNLPA
                     EDPQRFFEKIARYAREELEPQMREVSDAAAISFEKVGTTLSLDSAEQAAITRLVRTLC
                     NDFETRKVTYGTEAGLFSQAGIPTVICGPGSIDDAHKADESVELSQLAECDQFLRRVL
                     DTLTAP"
     gene            8166..>8740
                     /locus_tag="VHV99_22740"
     CDS             8166..>8740
                     /locus_tag="VHV99_22740"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HEX3638241.1"
                     /translation="MLPPVFAADMTRDNGSPVGDNQNSETAGPEGPVLLQDSHLIEKL
                     QRFDRERIPERVVHARGTGAFGEFVPSADISDLTTAQVFSPGSKTPVFVRFSTVMGYR
                     GSPEQARDPRGFAIKFYTRQGNWDMVGINWPIFFIRDAIKFPDFVHANKPSAVTGVQD
                     ANLAFDFFAHTPEATNMLTHLYSDEGMPDSY"
BASE COUNT         1724 a         2568 c         2751 g         1697 t
ORIGIN      
        1 gccggctttg tcggcgcgtt ccttgatggc aagcagtgtc tcatcgctgc accagtgcag
       61 gttggcaggt gcaagctgaa tccgggtcag cgattggcct tcgttgtcct gcgtgagcat
      121 gtcgaacaac tgcagatggt cggccagagg gacccgctgc gctttcagat gcgcggcgag
      181 cttcgctccg atgtccgccg gcaaacgttt aacgaagtct tcgtcggatt cgtagacgag
      241 caggttctgc tcgcgcacgg cgaagctgta cgaggcgcgc ataccgatgt cctgatacgc
      301 cttcagaacg cctgaagcgg cttcatgtat cgacgacaat ggacccggcc gccaaccgtg
      361 gatgtgctgc accgtggtaa tgccggacgc gatcatttcg aacgcggagt agagcgtgtc
      421 gagatagaga tcaatctcgc gcgtacccat ccggcttgca aaccacagtt ccagtgcgtg
      481 gtccggggag ccgagttgca gcggcgtcag gcccacgtga tggtgactgt tgacgaagcc
      541 tggcatcaac accgtatcgg gcgagccgag acgcggcgcg ttcggatact ttgcgaccag
      601 atcctgcgca ccgccgatct cgacgatcac gccgtcgcgt tgatacagcg cgccgtcgcg
      661 gatgacctca gcggtgtgcc ggtcggccac gcccttgacg atccagccag ccacaatcaa
      721 tgtttcggac atagttgctt ttacctgaat ggaggtgagt ttcagaacga tggtgtgtcg
      781 tatagatcga cacgccgatg gtcgaagtaa gagagccttt cgcgggtcgc cgcgatctgt
      841 gcatgcgcga gcgtcgcact gacaatgcca ggtccttcgt tgccaggccg tcgggtgagt
      901 acagcgccgt cggcggcaat cacacacgac tcgccatagt tgtcgatgcg cacgccatcg
      961 aggaattcgt caccgatctt gttcgcgcaa acgaccgcca gcccattttc ccgcgcgtgc
     1021 gtgcgcagct ccccgacgaa gaaatccatg tcgtcgccgc cgctgccggc gacgggcacg
     1081 atgacgagat cggcgccggc cgcacgcaac gcgcgccagc attctggaaa gcggcggtcg
     1141 tagcagacca gcacgccgat gcgccacgcg cctacctgca cgacgtgcag cgcgtcgcca
     1201 ggcgtgaagt gcgcggcttc gtcgtagccg ggcggcgggt cgtcgccgac aggcaggtga
     1261 agcttgcgcg caaccggacc ggcgcgcagc ggattgccgc cggcgtcgaa gccgagcacg
     1321 gcgttgtgat actggccagt agtccgatcg atttcgtaga acgggacgaa cagcgcgatg
     1381 ccacgcgtcc tggccagcgc ggcgcagcgc gcggcgaggg gaccatccgc gggctccgcc
     1441 cagtcacgat attgcccgcc cggcgcgctc gcgaagtacg gcaccatcga gagctcgggc
     1501 aagacgatca actgggcgcc gtcatcggcg gcggcgtgca cggcggcaaa tgccacggcc
     1561 tcgttgctgg caaggtcgct cgtgcaggga ggcaactgca gtgccgcgac ctgaagtgtg
     1621 ctcagggttg ccagccgatt catcggaccc gctcctgctc ggcgaaggcg cgatcgactt
     1681 catcgcgcat caagtcggtc aggtggacgc tgagatcgtg gaacgcccgg ctgcgcgtgt
     1741 ctcgcggacg aggaagatcg accggaatga tggtcttgat gcgccccggg cgtgccgtga
     1801 acacgacaat gcggtcggcg agcgcgatcg cttcgtcgat gtggtgagtg acgaagacca
     1861 tcgaaacatt gcttcgttgc cagacctgga tcaggaagtc ctgcatcttc gcgcgcgttt
     1921 gcacgtccag cgcggcgaac ggctcatcga gcagcagtat ctcgggtttc agcgcgagtg
     1981 cgcgtcccac tgctacacgc tggcgcatgc cgcccgagag ctggtcgggc cgtttgtcga
     2041 tggcttcgga caagccgaca cgctcgagaa tttcctctgc gatacgccgc cggttttcct
     2101 tgggcatgtg ctgcattttc aggccgaagg tgatgttttc gcccacgctc agccacggga
     2161 agagcgaggc gtcctggaag acgagtccac ggtccgtgcc cggtccgctc acctcggcgc
     2221 cgccgcacat gatgcgaccg ctggatacct gttccagtcc tgagatcaga tagagcaggg
     2281 tgcttttgcc acacccgctc ggaccgagga acacgacgaa ttcgcggcgc gcgatatcca
     2341 gctcgatatc acgcaaggca acgaatggtg cgccgtctgt ctgccatgtt ttgccgacgc
     2401 ccatgcaggt gacgagcggc ggttgcacgg aagctgattt gtgctggctc atatcatttc
     2461 cccaagccgt tatttggcac ccagaacagc accttgcgct gaacgattcg cagcgcaacg
     2521 tcgaaggcga agccgatgac gccgatcagc gtcatcgtga agaacgacag gctcgagttg
     2581 aaggtattgc gcgcgagcat gacgacctga ccgagcccgc tgccgacgcc ggtcgcttcc
     2641 gccaccagga ctaccatcca cgcgccgaag aggttcatgc gcagcaccat gaaaagcgac
     2701 ggcaaaatgg ccggcaggat cacatgacgg aacgtctgcg cgcgcgtcgc gcccatcacg
     2761 gcggcgacgt taaggtgggt acggctcacg ttgtcgatct ggctgatcgt cgagacggtc
     2821 atgacaaaaa acagcgagat gaacaccatg aatacagcgg gcgcgttacc gattccgaac
     2881 aggaagatcg cgaccggcag ccacgcgatt ggcgaaatgg gcgccagcag cgtgatcgtc
     2941 ggcaaggtga gattgccgaa caggcggaag tagcggatcg cgacgcccaa cgccacgctg
     3001 cagacgaagc ccagcaccag cccgcttaac acccgcaacg ttgatgcaac gatggtcatg
     3061 gcgagcgact tcagcgctgt cgcgtttgaa gcctcgccaa tcacgtcggt cggaatgaag
     3121 tactgcgctt gcgaggcgaa gttgcgcagg aagatatgcg gaggcggcag cagaagcgga
     3181 tctgcccaac ctgccgccca tgccagttcc catgcggcaa tgaagcacgt cagcgaggcg
     3241 acgccccagc caagcggcac gagtcgccgg acgaggttgg agcgtggccg gacacttgct
     3301 gccgggcgca ccacgcggcg gtgcgcgaag agggtcgtcg gatcggcttt catgcggatt
     3361 tcagcttgag ctggccgtag agatcggcgt tttccttgat cacctcattg agcggccccc
     3421 agtcgaacat gtcagggccg ggcagtcgct tgatgtagcg caggtccttc agattctgcg
     3481 cccgctgcag gatgaaattc tggttgctgc gctgatcgac catcgccggc tgcttcccgg
     3541 ctgcgtcgag cgtggcgtcg tagctcgttt tgaagtactt gccgacggtg tccttgacgg
     3601 ccgaggcgcg gtccagttct tcctgatgct gcgcggtcat cattgccttg atcagcgcct
     3661 tcaccgcatc gcggttttcg tgcaggagct tgctgcgtac tgcgagcacg cagtcggagt
     3721 agttcgcgcc gtatacatcc acaccgttcg aaagctgcac ggagcccggc cttgccttca
     3781 atgcctgcgt cgcgtaaggc tcgacgtggc acattgcatc cacgttcccg gcgatgaacg
     3841 cctgcgccag ctccggcgac gtgccgaagt agcgcacctt cacgtcggcg aagctcattc
     3901 cggctttctt caggtagtcg tagggcaaca tttcgagcgt atcggcctga aatgtgccga
     3961 tggttttgcc gcgcagttgc gccgccgacg tgatgcccgg ctgcgcgacg atcacgcagc
     4021 cctgcacgcc cgaacccgcg acgatcttga tcggtgcgcc ggcatcgtag agcgtcatga
     4081 agttggtgta cggaaccacg gagacgtcca ccacgcccgc gccgaagagc gccgcgatgt
     4141 ccgcattgga gggcgtggtg acgaagtcga gcgagacgcc ctgcgcgaga ttgcgttcct
     4201 tgacgagaaa gaatgggcaa ttgcagagac ccgtgccgtg cgtggccttg agggtcagcg
     4261 ggccggccgc catggcctgg ggaagatagg caccgcccaa cgccgcggcg ccggccagac
     4321 acgcaccgcg catcaggaat tcgcgtcgcg tactgccgag ttcggtcaag ggataaccat
     4381 tcgggtcttg ctgaacaaat ttcctgaaca tcgcgtggtc cttacagagg ttgaacggca
     4441 gatggggata tgaaagtttc cgcacgcgac tgcgcgagcg tttgttccag caggtcgaag
     4501 ctgaaaatct ctccgatcga gcctgaaatc tgcatgcgtc cgagcgcttc cagcgatcgg
     4561 gcacgggcga gaatgcacgc gcgaagcggg cgaatatcga cgctcggcgg ctgcgctgcg
     4621 atgccggcca gcagttcgtc gagcgtgaat tcggggtagc ggtgcgcgct ggcgcgcagg
     4681 gcttcgcggg gagtgttcgt gatcgcttcc tgcgcatcga gcatcgcgcg taaggccgcg
     4741 cggatgagct caggatgcgc gtcgagaaag ctcttgcgcg cgacgagcaa tgtgtcggga
     4801 tagtcgccgc cccaggcgtg caagccgtcg cacagtactt cgaagccgct cggccgaagg
     4861 cgcgaaatcc agggttcgat caacgttagc gccgcgaggc ggccggcgag cagatcgccg
     4921 actgcctcag cgagcgatgg gtacagtacg cgttcgatct gcatggagtc catgccgtgt
     4981 acccgcagtg catcgtgtag caggacttcc attggatcgt cggcgaaggt tccgacgcgc
     5041 tggccgcgca gatcgctcat cgcaaggccc ggctgcccca tgagcgccat accgagcttg
     5101 ccgctgccgc cgacaatgcg cagcggatcg ggctgggcat gatcgaccag cggctgtgta
     5161 aaaccggtgg tgccgagatc agcctcgccg cttctcagca atgcggcggt ggaaccgaaa
     5221 tgggtcaggg gcggtgcttc ggcgcgcaga ccgtggcgct cgaacaggcc gctatcgacc
     5281 gcaacaaaca acgcgagatt ggaaagcgca ctgccgtgcg ccagaaccag tttttgcatg
     5341 acgggccaag ggggcgttga gaatcgcgtc ggcagacggg ctgctggacg cgcgagcttg
     5401 accacgggtt agcgaagacc gtgccagcgg acgtcgatag gtgcgtactt tggcgtatcg
     5461 ctttgtttac aggatgttgt agatattgtt ctattaaaat ttataaaatt gtacacaatc
     5521 caaaatcgac ctatcttcga aagacgcacc acgatgtggc tgtaaggtca attttgcacc
     5581 ttcatgtcgc gctcggcaca gtggctgggc atcacgcgtt caagcctgtt tagttaggct
     5641 ttcaaggaag gacacgcaca gtcatacaga ttgccctcgt aactgaaata caatctggcg
     5701 tttaaattgt gtgcaatatc agccataatc tgtgttgcgg caaggttgtg taatcggtgc
     5761 attcaaccgc gcaggttgcc cttgtcgtga taactcatta cccaggaccc tttcatggct
     5821 actgataaga agagggcgcg gacagccgaa aacgaagtgg ctggctcctt cgacggcgcg
     5881 catcgccatg tcatttacga gcgcttgatg aatgcgattt cggagcatcg gctgcaaccc
     5941 ggcacgaagc tctccgaaga ccggctcgcc agtattttca atgtgagccg gaccacggtg
     6001 cgctcggtgt tgcagcaact gtcgcacgaa cttgtcgtga cgatcgtgcc gaatcgcggc
     6061 gcctttgttg cgagcccgac ggtggaggag gcgctcgagt tgttcgaggc gcggcgtcag
     6121 atcgaattgc cgatcatcga aaaggtgtgc cgttgcgcca cgaagaagga cctggcgcgc
     6181 ttgcgcgcgc atgtggcaca ggaagacgag gcccgtaagc gtgatgaccg gcgcaccacg
     6241 gtacggcttt caggcgaatt ccacgtgatg ctcgcgcagg tgtcgggcaa ccgcttcatt
     6301 gagcggacca tgcgcgggtt acaaatgctc acgtgcctga cgatcctgct ctataacacg
     6361 cctactgcgc aggcgtgtct gcacgacgaa cacgcgcgtt tgatcgacgc ggtggaggcg
     6421 ggcaacgtga agctggcaca aaaaatcatg gacgagcatc tcgagcatat cgaagcgacc
     6481 ttgcggttgc agggcccgga ggaagagagc gacgacctcg aggcgatcct cgttcatggc
     6541 ctgtgatggc gatccgattc agggcgccgt gagcgtgtcg agtacgcgcc tcagaaactg
     6601 gtcgcattcg gcgagttgcg acagttcgac ggattcgtcg gccttatgcg catcgtcgat
     6661 gctgcccggg ccgcaaatga ccgtgggaat tcccgcttgc gagaacaggc cggcttcggt
     6721 gccgtaagtc accttgcgtg tttcgaagtc attgcacaac gtgcgtacca gtcgagtaat
     6781 ggccgcctgt tcagcgctgt cgagcgacaa cgtggtgccg accttttcaa atgagatcgc
     6841 cgcggcatcg gagacttcac gcatctgtgg ctcgagttcc tcgcgggcat agcgggcaat
     6901 cttttcaaag aaccgttgcg gatcttcggc gggcagattg cggtattcga agaagaattc
     6961 gcaatcgtcc ggaatcgtgt tgatcgcgtt accgcccttg atcaggccgg tttgcgctgt
     7021 tgcatacggt acgtcgaagg cgcgatcgaa cgggccgttg cgacgcaggg catcggcggt
     7081 atcgcggatc cggcagatca ggcgcgcggc atattcgatc gcgttgacgc cgctcggcgt
     7141 acgcgacgaa tgggccgcca cgcccctcac gcaacaccgg taggcgttgt tgcccttgtg
     7201 ggcgacgacg ggccgcatgc tggtcggttc gccgacaatg cagcccgccg gcttgatgcc
     7261 gcgtttctca agttcagcca gaagcagggg cgcaccgaga cagccgacct cctcgtcgaa
     7321 cgagaaggcg aaatggacag gctcgcgtag ccggcgcttc tggatgtcgg gcagcaggcc
     7381 gagcgttact gcgataaacc ccttcatgtc gcacgagccg cggccgtaga gtcgatcgcc
     7441 gcggatgact ggatcgaagg gattacttga ccagttctgg ccggttacgg gaacgacatc
     7501 agtgtggccg gacagcacga cgcccccatt cgtctccccg ttaaaggacg gtagtgtcgc
     7561 gaacagattt gctttttgcc tggcccggtc atagaccagc agcgtttcga tgtgatgggc
     7621 tcgaaggcgg tcccggatcg cttcgatcag gccgagattc gaacggctac tcgtcgtgtc
     7681 gtagcgaatc agctcttccg tcaacgcgag ataggcgtgg cgttcgttag tgtccagtgt
     7741 tggcttgtca aacatcactt gtgccgtcgg gccctcggaa tgacatcgaa tgattgtacg
     7801 cggacgaaat tcacaacgtg tatgtcaatg aggttcccgc gaaattttct tctctccccg
     7861 caagtgttag aacccgggcc aaccgtttcc ttactattgg cataatgccg ccgtgcggtc
     7921 gcacgagttc ccggggcggt tctgttcgtt gcactgatat ccgcaagatt tgtcgcgcac
     7981 gcgcattccc aatgcatgac tatcgcgtgc attaggaatg gcgattggac cgctcaaagg
     8041 catcgacata gcatgcgatc tcgattagcg atgtcttgag tagtaactgt tcccaggaaa
     8101 caaatatgca acttgcaact aatgccttta aaagaacgct tttctccgtt gtcttgagcg
     8161 ccacgatgtt gccgccggtc tttgcagccg acatgacccg cgacaacggg tctcccgttg
     8221 gagacaatca gaattcggaa acggcgggtc ctgaaggtcc cgtgctattg caggattcgc
     8281 atctgatcga aaagctgcag cgtttcgatc gcgagcgtat tcccgaacgt gtcgttcatg
     8341 cgcgaggcac aggcgccttc ggcgaattcg ttccgagcgc cgacatatcc gacctgacaa
     8401 cagcgcaggt cttctcgccg ggcagtaaaa cgccggtatt cgtgcggttt tcaacggtga
     8461 tgggctaccg tggatctcct gaacaagcac gcgatccgcg cggtttcgcg atcaagttct
     8521 acaccaggca aggcaattgg gacatggtcg gcattaactg gccgatcttc ttcatccgcg
     8581 acgcgatcaa gttccctgac ttcgtccacg cgaacaaacc cagcgcggtc acgggggtcc
     8641 aggacgccaa tctggccttt gacttctttg cgcatacgcc cgaggcgacg aatatgctca
     8701 cgcatcttta tagcgacgag ggcatgcccg attcttacag
//
LOCUS       DATASH010000850        10963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5286730,
            whole genome shotgun sequence.
ACCESSION   DATASH010000850 DATASH010000000
VERSION     DATASH010000850.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 10963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10963
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5286730"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..366)
                     /locus_tag="VHV99_22745"
     CDS             complement(187..366)
                     /locus_tag="VHV99_22745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638242.1"
                     /translation="MNIHLHNADIVMIIALALLCSLLLALRFRPATWKGIVVEALAAN
                     AAAITAVIAFEMLLA"
     gene            complement(570..1877)
                     /locus_tag="VHV99_22750"
     CDS             complement(570..1877)
                     /locus_tag="VHV99_22750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3638243.1"
                     /translation="MSTSPISAAQPNATGQNSSARIIFASFIGTAIEFYDFYVYATAA
                     ALVIGPVFFPHGSATAQALSAFVTFGIAFVARPIGPFLFGHFGDRIGRKSTLVASLLV
                     MGVSTTLIGFVPGYDSIGSLAPILLCVLRFGQGIGLGGEWGGAALLATENAPAGKRGW
                     FGMFPQLGPSIGFLASNGLFFALALSLSDEQFRSWGWRVPFIVSAVLVALGLYVRLKI
                     AETPAFQAAIERKERVKVPIATLLSQHWRPTLLGALAMVVCYTLFYNATTFSLSYGVS
                     VLHIPRPTFLGMLCIAVVFMALATPLSAWASDRYGRKPVLIVGIIAAILSGFTMAPLL
                     GSGQTPQVLLFLVIELFLMGVTFAPMGALLPELFPTNVRYTGAGVSYNLGGILGASVA
                     PYIAQVLAAHGGLPWVGAYVSIAAAVSMIGVLCMRETRDARLM"
     gene            complement(2151..2573)
                     /locus_tag="VHV99_22755"
     CDS             complement(2151..2573)
                     /locus_tag="VHV99_22755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013338522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX3638244.1"
                     /translation="MAGCTITPPQRPLIITPAAAVNSATLDQYRSAVAKRIIERNPSY
                     VLRGTPQAMLRSLVVVSFTVDREGRVLESSVYRTNGDDEAESTALATLRRASPLPQPP
                     GKLLNGHGQVELFEDWLFNDNGKFQLREFASPQAQTID"
     gene            3108..10658
                     /locus_tag="VHV99_22760"
     CDS             3108..10658
                     /locus_tag="VHV99_22760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEX3638245.1"
                     /translation="MEHSSFALTRTQSRTLAHAAGPNLCAVYRIDGRPDVERLSAAVA
                     QLVVHCRPLAYRLVETEQGLRWAIRREVCAELNVIDLDSRDARDEAAALSLIDGLCAR
                     AFRMDAGLPWALTLLRDGERSYLVFACHAALLDRFSLKPLFNALSWFYCGEAPGAPLD
                     IDQQGLLDAERELLASDRLQIDLDFWIGQVGDASFEWHPPRRQNARADSSFSVRLDAQ
                     TGAALRSQAKALGIALDVLIKLCVHVLLHRMSGSRAVLTAHHQRGRGRHDETIGFDER
                     RRLLKTDFDDTMTLRQFLRHAAARAEMADFHAELPAFEVLHDIERREPGFVRATNVVC
                     ETDSLPYDALTLDALPVALLAAYSRRFAHEEIGVYLDVRERIALHVHSRHPQELDGLR
                     LAFEHLTALLARVGECLGQKLDAIDLYTPALRQRCAQWSDGGPLAAPARDVLDLFAQA
                     ALEHAGRPALSGPDAVFTYAQLALAAQRVGAALAMRAPAAPDPAAPDPCAPDPSAPDP
                     CAPDPAAPDSCAPDPSAPDPSAPDGRDTLIGICVSRGARIVPAMLGVLASGAGYLPLD
                     PQMPDERLRYIVEDAQLQAVIVDAATHERLSGLVDCALYRLDDLLDAPVAEARASRLA
                     SRLDSRSTPADPQRAAYVIYTSGTTGKPKGVVIERGMLAHFIASLTGRYRRDESTRWL
                     QFASVNFDASVMEIFNPLTHGGHLIVAPEAVRTDAEALSGFLDAERITHAFLPPAILK
                     LLPRRALPHLDTILCGGEASDDETIRFWSGVLRLSNIYGPTEATVMATDNTFDGDKPA
                     NQLGHPLPGYQIHLLDEEGEAVPLGAIGEICIGGAAVSRGYLQRPELTGQKFRGNPFG
                     PGRLYRSGDLGRFQPDGAIEFLGRSDFQVKIRGYRIELGEIEASIVEQPEVRSAYVGT
                     CERHGGTALVAWYVGASLEPAVLRHRLAQRLAHYMVPAFLLPIEALPLTLNGKIDRAR
                     LPAPQAGPADPANRAFDAFEQAVQGIWSEVLGVPLESVGEHSHFFQLGGHSLLATLVC
                     NRVSLNLHAPVKLKTLFEHPRFDAFCARARQGVEQGAMLPPLVAQPGEPMPVPVTSRL
                     VRMMHTRTLSEPDDNAYNIVMRVDFGGAANPLRLHQALVAVLTHNPIFNMSFAERDGR
                     LWMLPGTAVESPHGLIPLESCAASAIDARIDAMRALSLPLDCAPPWRAQLLMSPLENR
                     TSLLLAMHHAMFDGWSCKLLLDELTARYEGKPVSGQPLSWFDYGHWAPQLAASPRFAA
                     ARHYWMRKLDAVALRTELPFDALQREPNANRALKLQVRPDQMRRLRLIAEAHDMTLPP
                     VVFALYLVWLWRVSGQQHLACAYPYAGRDVPGSEGIYGMFVSMAVLVQHIDTRQGFGE
                     LARAVQRQMLDDRDHLLATPYDTDNAQLGALNTIFSLQNGIELNGRVGEAPYHAEEYP
                     SLTSKADLSAVFYPRPDGGLDGRFEYDSSVLHAASVEQMAEVFMTLCDAAGHQPDALI
                     GELDYLGDGQHAQCLGFACGEPLPDAPQSIPARFAEVVAANAARVAVRCDAEQLSYRE
                     LDALSDAIASGLTACMPGGARIGLSMQKSVLLVASVLGVLKAGCAYVPLDPAYPAERL
                     RYFVENCSVDTVLADETSRRALEAAGLGELRTLDPAQVAKGQPKALTQDARDTQAGAD
                     VQASTDPRADAQKQAMRPRRPSAVGPEALAYVIHTSGSTGKPKGVLVEHHSVVRMVAG
                     ASRALNYAPGSISTLAASTNFDASVLDLFLALLHGGTLIVLPEEARRDPRLLHSLLKA
                     ERVTHASLAPVVVQSLPREPLPDLQLLGFGGDTLDEPSAAWWSEHTRLFSLYGPTEIT
                     VMASCGQVLPGGPSRIIGKPLPGYRLYLLNAQRQLAPPGTVGEIYIGGENLARGYINQ
                     SAMTMERFVVDPFRDAPYALMYRTGDLGRFLADGTIEYFGRNDAQIKLRGFRIELGEI
                     ENCIAAAPGVAHAACAVWGEGEARYLAAYYVAAPGEEGAAPDEDTLRRHVGEALPDYM
                     VPACFIRLDALPASPNGKHDRKALPAPSRISGTPPRAGVEQRIAEIWQALLRVTAIGR
                     DDSFFRLGGNSILAVRMQGQLRDALGIEFPMAAFYRSPTIATLANGSHDDMIERALAD
                     AAQPFVVEHAAPVVSDSAGATPIRTMLLTGASGFLGIYLLHALCGRLDKVICLQRCPN
                     AAAGRAVLQDKAREAGLAIDFSRVEVIPADLAVPQLGLDTPRWRQLAGEVDAILHCGA
                     FVHHLHSYEEMKGANVGGTEALLRLALTQRRKPFCFVSTLSVGEMLADARYIDEQVSD
                     ARPLLDNGYLLTKWVGEQRVAECVRRFGLPAVIARPGNITGDSRTGFSNFAHNHFWLF
                     NKGCLQLGAYPRTAQEVEMMPVDLLAGAMTAMALHPYAGLRVANLNNPLALSQRQWFA
                     ALAACGLRAEPQAAADWQRRLANLDASNGLALIRDFYTGDLSAAPLPVEQIGTLTELA
                     RHGVDLTVDYGALIRLYVGYLREAGFLHSLPETAVP"
BASE COUNT         1890 a         3495 c         3598 g         1980 t
ORIGIN      
        1 gaacaatatg aaacgcttaa tcctgactct cgtcacaggc acgctgctcg caacggcgct
       61 tggaggttgc atcgttgcac cggcgcctgg ctattactac ggcggcggtg gttattacca
      121 tcacggtggc tactactacc gctaatggct tgcggcgccc atccgggcgc cgccgcttcc
      181 gaacgctcag gcgagcagca tttcgaaagc gatcaccgcg gtgatcgcag ccgcgttcgc
      241 ggccagcgcc tccacgacaa tgcccttcca cgtcgcggga cgaaaacgca aggccagcag
      301 cagcgaacac agcaacgcca gtgcgatgat catcacgatg tccgcgttgt gaagatgaat
      361 gttcatcaaa gcctccctgc tcgccatagg gttattggat tcgagtatag gcaagtcaaa
      421 aagacgcgca aggcagagga tttaccagca cagtgtgcaa aacggggaga aagacgggat
      481 gaccgccgat gcggccgcag cgcgcgtcaa ggcgcgcctg gcggcatcgg ggtaaagaag
      541 caatcagacc atcagcgcag cgcgcccgct tacatcaagc gcgcatcgcg cgtttcgcgc
      601 atgcacagca cgccgatcat gctgacggcc gcagcgatcg acacatacgc gcccacccac
      661 ggcaacccgc cgtgcgcggc cagcacctgc gcgatatacg gcgcgacaga cgcaccgaga
      721 atcccgccga ggttgtacga cacgcctgca ccggtatagc gcacgttggt cggaaacagt
      781 tccggcagca gcgcgcccat cggggcgaag gtcaccccca tcaggaacag ttcgatcacg
      841 aggaacagca gcacctgtgg tgtctgtccg ctgccgagca acggcgccat cgtgaagccc
      901 gacaggatcg ccgcgatgat gccgacgatc agcaccggct tacgcccgta acggtcgctc
      961 gcccaggccg acagcggcgt ggcgagcgcc atgaacacga ccgcgatgca cagcatgccg
     1021 aggaaagtgg gccgcggaat atgcagcacc gacacgccgt acgacagcga aaacgtcgtg
     1081 gcgttataaa acagcgtgta gcagaccacc attgccagcg cgccgaggag ggtcggccgc
     1141 cagtgctgcg agagcagcgt ggcgatcggc accttcacac gttccttgcg ttcgatcgcg
     1201 gcctggaagg ccggcgtctc ggcgattttc aggcgcacat acaagccgag cgcgactagc
     1261 accgcgctga cgatgaacgg cacgcgccag ccccagctgc ggaattgttc gtcgctcaaa
     1321 gacagcgcca aagcgaagaa cagaccgttg gaggcgagaa acccgatcga cggccccagt
     1381 tgcgggaaca tcccgaacca gccgcgtttg ccggccggcg cgttctcggt ggcgagcagc
     1441 gccgcgccgc cccattcgcc gccgaggcca ataccctggc cgaagcgcag cacgcacaac
     1501 aggatcggcg caaggctgcc gatcgagtcg tagccgggca cgaaaccgat caacgtggtc
     1561 gacacaccca tcactagcag cgaagcgacc agcgtcgact tacggccgat ccggtcgccg
     1621 aagtgaccga acaggaacgg gccgatcggc cgcgcgacga acgcgatgcc gaacgtgacg
     1681 aaagccgaca acgcctgggc ggtcgccgag ccgtgcggga aaaacaccgg tccgatgacg
     1741 agcgccgcgg cggtcgcata gacgtagaaa tcgtagaact cgatcgcggt gccgataaag
     1801 ctcgcgaaga tgatccgcgc gctgctgttc tgcccggttg cgttgggctg ggccgcggaa
     1861 atcggcgaag tggacatgca gggtctccta tgtcgtgatt cgtgatgccg ttgggcgccg
     1921 tgctcgtatg cgggcggcgc gtggtcgggc ggcatctttg tttggattcg tgcgcttcgg
     1981 cgcgtcttgc tggtggttaa agccatgctc ggcgcaagcg acgaagcgac agcttacaac
     2041 ggcagccggc cgacgtcagc ggtcggatga gattttagcg ctgctttaaa gccgcggtgc
     2101 cgccgcttgc gctgcgttag aagcgttcgg aaaccgcttt ggcgtcgcgt tcagtcgatg
     2161 gtttgcgctt gcggcgaggc gaactcgcgc aactggaatt tgccgttgtc gttgaacagc
     2221 cagtcttcga ataattcgac ctggccatgg ccgttcaaca atttgccggg cggctgcggc
     2281 aatggcgaag cgcgtcgcaa ggtggcaagc gcggtgcttt cggcttcgtc gtcgccattg
     2341 gtgcgataga ccgacgactc cagcacccgg ccctcgcgat ccaccgtgaa cgacaccacc
     2401 accagcgaac gcagcatcgc ttgcggcgtg ccgcgcaata cataggaagg gttgcgttcg
     2461 atgatgcgtt tggcgaccgc gctgcggtac tgatcgagcg tcgcactgtt gacggccgcc
     2521 gccggcgtga tgatcagcgg gcgttgcggc ggcgtgatgg tgcagcctgc catggctgcg
     2581 agcgtcagaa tcgaagcggc cgtcaatgcc gctgtcaatg ccgatgccgc gtgatgcgca
     2641 tgcgatagag acgcgctcac gcgtgaggcg aagcgtccgg tcaagcggcg aaggtggcgc
     2701 gtgcggatga gcatcgtcgt ggaaagcatc ggggcgggtc taacttgatg gtagtcaaaa
     2761 agcgccaggt ttcaattgtc gttcaccctg tatggcaatc ccaaaccacg tggcccggtt
     2821 cgctcgcacg ccatgcccag ggatctttcc attgcctttc tgctgcatgc atgagtggct
     2881 tcagtctgat gcgatttacc tgacatgatc cagctttaag caaacgtcct cacgctagtc
     2941 cgcacataac aggagttcta agagtcttcc tatggcgact cgatcctcac gtcaatagcg
     3001 tgtctgatcg gacgatcggg cgaagcgccg cggcgcggtc atgagccgtg cgcggccggc
     3061 gcgacgcgag ccgatcggac ctgtccatcc cgacaacgag gtatcgtttg gaacactctt
     3121 cctttgcgtt gacgcgcacg caatcccgca cgctggcgca cgccgccggt cccaatctct
     3181 gcgccgtcta ccggatcgat ggccgtcccg acgtcgagcg tctgtctgcc gcggtggcgc
     3241 aactggtcgt gcattgccgg cctctcgctt accgcctcgt cgaaaccgag cagggcctgc
     3301 gctgggccat tcgccgcgag gtgtgcgcgg aactgaacgt catcgatctc gacagtcgcg
     3361 atgcgcgcga cgaagccgcc gcgctgtcgc tgatcgacgg cctttgcgcg cgagcctttc
     3421 gcatggatgc cggactgccg tgggcgttga cgctgctgcg cgacggtgag cgaagttatc
     3481 tcgtgttcgc ctgtcatgcc gccttgctcg accgcttttc gctgaagccg ttgttcaacg
     3541 cgctttcgtg gttctattgc ggcgaggcgc ctggcgcgcc gctcgacatc gatcagcaag
     3601 gcctgctcga cgccgagcgc gaactgcttg cgagcgaccg tttgcagatc gacctcgatt
     3661 tctggatcgg ccaggtgggc gatgcctcgt tcgaatggca tccgccgcgc cggcagaacg
     3721 cgcgtgccga cagcagcttc agcgtgcgcc tcgatgcgca aaccggcgct gcattgcgca
     3781 gccaggcaaa agcactcggc atcgcgctgg atgtactgat caagttgtgc gttcacgtgc
     3841 tgctgcatcg catgagcggc agccgcgcgg tgctcaccgc gcatcaccag cgtggccgcg
     3901 gtcggcatga cgagacgatt ggtttcgacg aacggcggcg cttgctgaaa accgacttcg
     3961 acgacacgat gacgctgcgg cagtttctgc ggcatgcggc ggcgcgcgcg gagatggcgg
     4021 attttcatgc cgagctgccg gcctttgaag tgctgcacga tatcgagcgg cgcgagccgg
     4081 gtttcgtgcg cgcgaccaat gtggtgtgcg agaccgacag cttgccctat gacgcgctta
     4141 cgctcgacgc gctgcctgtg gccttgctgg cagcgtattc gcgccgtttc gcgcatgagg
     4201 agattggcgt ctatctcgat gtgcgcgaac ggatcgcgtt gcatgtgcat agccggcatc
     4261 cgcaggagct cgacggcttg cgcctcgcct tcgagcattt gacggcgttg ctggcgcgcg
     4321 tcggcgagtg tctcggacag aagctcgatg cgatcgatct ctatacaccg gcattgcggc
     4381 agcgttgcgc gcaatggtcc gatggcggcc cgctcgcggc gccggcgcgt gacgtgctcg
     4441 atctgttcgc tcaggcagcg ttggaacatg cagggcgtcc ggcgctcagc ggcccggacg
     4501 ccgtcttcac ttacgcgcaa ctggcgctgg ccgctcagcg cgtgggcgcg gcactcgcca
     4561 tgcgcgctcc agcggcaccc gatccagccg cacccgatcc atgcgcaccc gatccatccg
     4621 cacccgatcc atgcgcaccc gatccagccg cgcccgattc atgcgcaccc gatccatccg
     4681 cgccagatcc atccgcgccg gacgggcggg acacgctgat cggcatttgc gtctcgcgcg
     4741 gcgcgcgtat cgtgccggca atgctcggcg tgcttgccag cggcgcgggc tatctaccgc
     4801 tcgatccgca gatgcccgat gagcgtttgc gctatatcgt cgaggatgcg caactgcaag
     4861 cggtgattgt cgatgcggcg acgcacgagc gcctgagcgg gctggtcgat tgcgcacttt
     4921 atcggctcga cgatctgttg gacgcgccgg tggccgaggc acgcgcttca cgactcgctt
     4981 cacgactcga ttcacgatct acgccggccg atccgcaacg cgccgcctac gtgatctaca
     5041 cctccggaac gacgggcaaa cccaaaggtg tggtcatcga gcgcggcatg ctggcgcatt
     5101 tcatcgcctc gctgacgggc cgctaccggc gtgatgaaag cacccgctgg ctgcagttcg
     5161 catccgtcaa tttcgacgcg agcgtgatgg agatttttaa tccgttgacg catggcggcc
     5221 acctgatcgt cgcacccgaa gcggtgcgca ccgatgccga agcgctgtcc ggcttcctcg
     5281 acgccgagcg catcacgcat gcattcctgc cgccggcgat tctcaagctg ctgccgcgcc
     5341 gcgcgctgcc gcacctggac acgatcttgt gcggcggcga agcgagcgac gacgagacga
     5401 tccgcttctg gtccggcgta ctacgcctgt cgaacatcta cgggccgacg gaagccaccg
     5461 tgatggcgac cgacaacacc tttgacggcg acaaaccggc caatcaactc ggccacccgc
     5521 tccccggcta ccagatccac ttgctcgatg aggaaggcga agcggtgccg ctcggcgcta
     5581 tcggcgagat ctgcatcggc ggggcggcgg tgtcgcgcgg ctatctgcag cggcccgagt
     5641 tgaccgggca gaagttccgc ggcaatccat tcggcccggg ccgtctttac cgttccggcg
     5701 acctcggccg cttccagccg gacggcgcga tcgagtttct cggtcgtagc gattttcagg
     5761 tgaagattcg tggctatcgg atcgaactcg gcgagatcga ggccagcatc gtcgagcagc
     5821 cggaggtgcg cagcgcgtat gtcggcacgt gcgagcggca cggcggcacg gcgctggtgg
     5881 cgtggtatgt cggggcatcg ctggagccgg ccgtgctgcg gcatcgcctt gcgcaacgtc
     5941 tcgcgcatta catggtgccg gcattcctgc tgccgatcga ggcgctgccc ctgacgttga
     6001 acggcaagat cgaccgcgcc agattgccgg cgccgcaagc cggtccggcc gaccccgcga
     6061 accgcgcttt tgatgccttc gaacaggcag tgcaaggcat ctggtcggag gtgctgggcg
     6121 tgccgctcga aagcgtcggc gagcacagcc atttcttcca gttgggtggc cactcgttac
     6181 tcgccacgct cgtgtgcaat cgcgtcagtt tgaacttgca cgcgccggtc aaactgaaga
     6241 cgctgttcga gcatccccgg ttcgatgcgt tctgcgcgcg ggcgcgccaa ggtgtcgaac
     6301 aaggcgccat gctgccgccg ctcgtcgcgc agccaggcga acccatgccc gtgccggtga
     6361 caagccggct cgtgcggatg atgcacaccc gcacgctcag cgagccggat gataacgcct
     6421 acaacatcgt gatgcgggtg gatttcggcg gcgcggcgaa tccgctgcgg ttgcaccagg
     6481 cactcgtcgc cgtgctgacg cacaacccga tcttcaacat gagcttcgcc gaacgcgacg
     6541 gacgattgtg gatgctgccc ggcacggccg ttgaatcgcc gcatgggcta attccgctgg
     6601 aaagctgtgc cgcctctgca atcgatgccc gtatcgatgc catgcgcgcg ttgagcttac
     6661 cgctggactg cgcgccgccg tggcgcgcac aactgctgat gtcgccgcta gagaaccgca
     6721 ccagcttgct gctcgccatg catcacgcga tgttcgacgg ctggtcgtgc aagctgctgc
     6781 tggatgaact gacggcacgc tacgaaggca agccggtttc agggcaaccg ctctcgtggt
     6841 tcgactacgg ccattgggcg ccgcaactcg cggcatcgcc gcgctttgcc gccgcgcgtc
     6901 actactggat gcgcaaactc gacgctgtcg ccttgcgtac cgaactgccg ttcgacgcac
     6961 tgcaacgcga gcccaacgcg aaccgcgcgt tgaagctgca ggtgcggccc gaccagatgc
     7021 ggcgcttgag gctgattgcc gaggcgcacg atatgacttt gccgcccgtg gtgttcgcgt
     7081 tgtacctcgt ctggctgtgg cgcgtgagcg gtcaacagca cctggcttgc gcttatccgt
     7141 atgcgggccg cgacgtgccg ggcagcgagg gcatctacgg tatgttcgtc tcgatggcgg
     7201 tgctggtgca gcacatcgac acgcggcagg gtttcggcga actggcgcga gccgtgcagc
     7261 gtcagatgct cgacgaccgc gatcatctgc tcgccacgcc ttacgacacc gataacgccc
     7321 agctcggcgc gctgaacacg attttcagct tgcagaacgg tatcgaactg aatggccgcg
     7381 tcggcgaggc gccttatcac gccgaggaat acccttcgct gacgtcgaag gcggatctgt
     7441 cggcggtttt ctatccgcgg ccggacggtg gactggacgg gcgcttcgaa tacgacagtt
     7501 cggtgctgca tgcggcctcg gttgagcaaa tggccgaggt gttcatgacg ttgtgcgacg
     7561 ccgcgggtca ccagccggac gcgctgatcg gcgaactcga ctatctgggc gacgggcaac
     7621 acgcacagtg tttgggcttc gcttgcggcg agccgctgcc ggacgcaccc cagtcgatac
     7681 ccgcgcgttt cgccgaggtg gtcgcggcga acgcggcgcg cgttgcggtg cgctgcgacg
     7741 cagagcagct cagctatcgc gagctcgacg cgctcagcga tgccatcgcc agcggcctga
     7801 ctgcctgcat gccgggcggc gcgcgcatcg gtttgagcat gcagaagagc gtcttgctag
     7861 tggcgagcgt gctgggcgtg ctcaaggcgg ggtgcgcgta tgtgccgctc gatcccgcgt
     7921 atcccgccga gcgcttgcgc tatttcgtcg agaattgttc ggtcgatacg gtgctcgccg
     7981 atgaaacgag ccgccgcgcg ctggaggccg ccggtctcgg cgaattgcgc acgctcgacc
     8041 cggcgcaggt ggcgaagggg cagccaaagg cattgaccca ggacgcgagg gatacgcaag
     8101 cgggagcgga cgttcaggcg agcacggacc cgcgcgcgga cgcgcagaag caggcgatgc
     8161 ggccgcgccg gccgagcgcc gtcggcccgg aggcgctcgc ctatgtgatc cacacctcgg
     8221 gctcgaccgg taagccgaaa ggcgtgctgg tcgaacacca tagcgtcgtc cgaatggtcg
     8281 ccggcgcaag ccgcgcgctt aactatgcgc cgggcagcat cagcacgctt gcggcgtcca
     8341 ccaacttcga cgccagcgtg ctcgatctgt ttctcgcgct gctgcacggc ggcaccctga
     8401 tcgtgctgcc ggaggaggcg cgccgcgatc cgcggctgct gcacagcttg ctgaaggcgg
     8461 agcgcgtcac gcatgcatcg cttgcaccgg tggtggtgca gagcttgccg cgcgagccgc
     8521 tgcccgactt gcagttgctc ggcttcggcg gcgatacgct tgacgagccg agcgccgcct
     8581 ggtggagcga acacacgcgc ctcttcagcc tgtacggccc caccgaaatc acggtgatgg
     8641 cctcgtgcgg gcaagtgctg ccgggcggcc cgagccgcat catcggcaag ccgctgccgg
     8701 gttaccggct ctatttgctc aatgcgcagc ggcaactggc gccaccgggg acggtcggcg
     8761 aaatctatat cggcggcgag aatctggcgc ggggctacat caatcagtcg gccatgacga
     8821 tggagcgttt cgtggtcgat ccgtttcgcg acgcgccgta cgccctgatg tatcgcaccg
     8881 gcgacctcgg ccgctttctc gcggacggca cgatcgaata tttcggccgc aacgacgcgc
     8941 agatcaagct gcgcggtttt cgcatcgaac tcggcgaaat cgagaattgc atcgcggccg
     9001 cgccgggcgt cgcgcatgcc gcttgcgcgg tgtggggcga gggcgaggcg cgttatctgg
     9061 ccgcgtatta cgttgccgcg ccgggtgaag aaggcgctgc gcccgacgag gacacgctgc
     9121 gtcgtcatgt gggcgaggcg ctccccgact acatggtgcc ggcgtgtttt atccggctgg
     9181 acgcgctacc ggcatcgccg aacggcaaac acgatcgcaa ggccttgcct gcgccatcac
     9241 gcataagcgg tacaccgccg cgcgcaggcg tcgagcaacg catcgctgaa atctggcagg
     9301 cgctgctgcg tgtcaccgct atcggccgcg acgatagttt cttccggctc ggcggcaact
     9361 cgattctggc ggtgcggatg caggggcaat tgcgcgatgc gctcggtatc gagtttccga
     9421 tggctgcgtt ttaccgctcg ccgaccatcg ccacgctcgc gaatggcagt cacgacgaca
     9481 tgatcgaacg cgcgcttgcc gacgcagcgc agccgttcgt tgtcgagcat gccgcgcccg
     9541 ttgtttctga cagcgccgga gcaacgccga ttcgcacgat gctgctgacc ggcgcgagcg
     9601 gcttcctcgg catttacctg ctgcacgcgt tatgcggacg gctcgacaag gtgatctgcc
     9661 tgcaacgctg tccgaatgcc gcggcagggc gcgccgtgtt gcaggacaag gcgcgcgagg
     9721 ccggcctggc gatcgacttc agccgcgtcg aagtcatccc tgccgatctt gccgtgccgc
     9781 agcttggact cgacacgccg cgctggcggc aactcgccgg cgaagtcgac gcgatcctgc
     9841 attgcggcgc gttcgtgcat cacctgcata gctatgagga gatgaagggg gcgaatgtcg
     9901 gcggcaccga ggctttgctg cggctcgcgt tgacgcagcg gcgaaagccg ttctgcttcg
     9961 tctcgacgct gtcggtcggc gagatgctgg cggatgcccg gtacatcgac gagcaggtta
    10021 gcgacgcccg cccactgctg gacaacggct atctgctgac gaagtgggtc ggtgagcagc
    10081 gggtggcgga gtgcgtgcgt cgcttcggtt tgccggcggt gatcgcgcgg ccgggcaata
    10141 tcaccggcga tagccgtacc ggcttcagca acttcgcgca caaccatttc tggctgttca
    10201 acaaggggtg cttgcagttg ggcgcctatc cacggacggc gcaagaagtg gaaatgatgc
    10261 cggtcgatct gcttgccggc gcgatgaccg cgatggcact gcatccgtat gccggcttac
    10321 gggtggcgaa cctgaacaat ccgctcgcgc tcagccagcg gcagtggttc gcggcgctgg
    10381 ccgcttgcgg tttgcgcgcc gagccgcagg cggcggctga ctggcaacgg cggctggcga
    10441 atctcgatgc gtcgaatggg ctggcgctga ttcgcgactt ttataccggc gatctgtccg
    10501 ctgcgccctt gccggttgaa cagatcggca cgctgacgga gttggcgcgg cacggcgtgg
    10561 atctgacggt cgactacggc gcgctgatca ggctctatgt ggggtatctg cgcgaggcgg
    10621 gttttcttca ctcgttgccg gagacggccg tgccctgaag cgacgggcgg tgcatccgcc
    10681 cgtttcagct tgcacaccga acccgtgcgt gctttgtaat aggctgaaat atgagctgtt
    10741 ttctcttctg cccaaacacg cctacacttc gttggcctcc ttcgagaggc atcggtggaa
    10801 gtctttcatt tagcccgctt cgcgcgggct ttcttttttg cgcgacgctt tgcgccatcg
    10861 atcctgccag ccagccgcga aattgaaaaa ggcgaacccg tcgatgagtt cgcctttgtt
    10921 tgcgtgcttc gtttagtgcc cgcagtgacg cccgaggtaa tgt
//
LOCUS       DATASH010000851         5850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6472301,
            whole genome shotgun sequence.
ACCESSION   DATASH010000851 DATASH010000000
VERSION     DATASH010000851.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5850
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6472301"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1460
                     /locus_tag="VHV99_22765"
     CDS             <1..1460
                     /locus_tag="VHV99_22765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638246.1"
                     /translation="SRRAPARSHCRGLEMNYELLFRAGAHAAMRAGNGASAPGTPEVR
                     YALTGAKGGFARTLLAQTRLMPRLAPTLLCDLDVAGLRALCEELGYPAADLVECSTPD
                     EVAALPQNAIGLIADVALLDPARYNILVEATGNPSIGYRCARRALQHKRHVAMVSKEV
                     DAVAGVSLAQLAAREGVVYTTADGDQPSNLIGWVSWARTLGLEVVAAGKSSEYDLIFD
                     PATHSVTQLDETFAAPELAGLMKLGDDVRATLAARRAALNGRKTSATADYCEMAVVAT
                     STDLIPDVELLHYPVARTTELADIYALQEDGGVLGKRGVIDVFNMLRLPDEASFAGGV
                     FVVVRTHDAPTWALLAQKGHVVSRNGRYACMYLPYHFMGVETPISLLDAVLHGRPSGA
                     ETPRQCTILSGRASVDLPAGTVLKMGGHHHDVTGVQAVLIARNDAPRDIAPLYLAAHA
                     TLARDVKAGVLLSVDDIAGYDAELLSAWQTGFTPA"
     gene            1547..2896
                     /locus_tag="VHV99_22770"
     CDS             1547..2896
                     /locus_tag="VHV99_22770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015295 - solute:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate:H+ symporter"
                     /protein_id="HEX3638247.1"
                     /translation="MHGDAVTIVMAVIAIAVLVVLIARFKLSPFVALTLTAIGLGIVS
                     GVTPEHTVDAFAKGFGGALARTGPVVGLGALLGGILIGTGGADRIANAFIGTRPIYYA
                     PWTVCAAALLIGMPHLFDVSFIMLAPLVYTVAKRTGSHLLYIGLPLAAGLYVSHGLLP
                     PHPAPMLAVVAYHANTGLTLAYGLLIAIPTAVICGPLFTRVIRPYFGPAPDLNTGPVA
                     ASEPAANRPQVSLALSVTSVLIPPGLMLIGTMGMAYSVKGSLAYILFQSVNDPILSLL
                     AAVIFAFFALGVRGGFTLVDVQKMASKGLGPLGVILLILGAGGGFKEMLSAIHLDVII
                     VHHTAGWAVNPLVLAWAIAALLRICVGSATVATVAASGIVAPLLLSHPGVSPELMVLA
                     TASGAVMLSHVNDSGFWLFKEYFQLTVAQTLRTWTLMLTLQSFVGLAGVLLLETVIR"
     gene            2987..3805
                     /locus_tag="VHV99_22775"
     CDS             2987..3805
                     /locus_tag="VHV99_22775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008346923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEX3638248.1"
                     /translation="MPSHTEPGASAALDTPLIPDQRRESMLRQLRRHKVLSVHQLTVM
                     FDCSHMTVRRDIAVLEQAGLVYSVPGGVRIASQLNSEPSHQSKALIEQPQKQAIAVCA
                     AAFLRAGMTAYLDAGTTTLTLVPHILALSDMTVVTNDFPIVQELAEATHVNVIHTGGM
                     LDHANRSGVGGLAAATLRHIAVDVAFISASSWDLRRGVTTPSAPKVEVKQAAMAAAAQ
                     TVLLATSSKYGTFGLYKVARLEQFDHVISDEGLTEAAAVGIREAGVDLTVAKGE"
     gene            3955..4857
                     /locus_tag="VHV99_22780"
     CDS             3955..4857
                     /locus_tag="VHV99_22780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018434280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3638249.1"
                     /translation="MENLLKKLDLTSLRLFVAVCQEQNIARAAEREFIASSAVSRRIA
                     EIEAVIGLPVIQRQSRGITVTPVGETVLRYALAIIGNIEQMSAELSRFSSGAKGRVRV
                     VANLSSIVQFLPEDVAAFGRMFPEVSIELEEENSTDVLRIVDEHGADFGICNPVAGSE
                     AFEQVPYREDRLAVLVPGGHRLAGVPRVAFEELLDESFVGLRSESALTKLLAQQALSA
                     GRQLDVKIRVSSLDALCRMVHAGLGIAIVPEQVGLLYVNALDVRLLSLSDIWAVRHLI
                     MIFKSRDQLSASTAALVGFLGSKP"
     gene            5032..>5850
                     /locus_tag="VHV99_22785"
     CDS             5032..>5850
                     /locus_tag="VHV99_22785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HEX3638250.1"
                     /translation="MSDTIRLVGRVLYLSQDPAVIEAQLAGENFTRVSAGPLRDNVST
                     DEITPVTVMLTYDERLGQYPYVGFKAGERMPIGRNAIKDGGFQVTVAGKRYGKGSSRE
                     SSPLAELSAGIRLIVAESFERIYQQNCDNIGILTTTDFSVLDRLIAGEAVPIDEFLKG
                     RDALTQQIIRSGGLLAYSKFADWPAPRVRETANLAPTAAPANTTHATNATTATNATNA
                     TNTPEPKTLVEKIIERHLHPGIESAQRGDGVFIAADWRFSHDYFTGMCAHLMHRA"
BASE COUNT          990 a         1850 c         1935 g         1075 t
ORIGIN      
        1 ggtcgcggcg cgcaccggcg cgcagtcatt gcaggggact tgaaatgaac tacgagttgc
       61 tgtttcgcgc cggcgcccac gccgccatgc gtgccggaaa cggcgcatcg gccccgggaa
      121 cgccggaggt tcgctacgcg ctgaccggcg cgaagggcgg cttcgctcgc acgctgctcg
      181 cacaaacgcg cctgatgcca cggcttgcgc cgacgctgct gtgcgatctg gacgtcgcgg
      241 gcctgcgcgc gttgtgcgag gagctcggtt acccggctgc ggatctggtc gaatgcagca
      301 cgccggacga ggtcgccgca ctgccgcaga acgccatcgg gctgatcgcg gacgtcgcgt
      361 tgctcgaccc tgcgcgttac aacattctcg tggaagcgac cggcaatccg tcgatcggct
      421 atcgctgcgc acgccgcgcg ctgcagcaca agcgtcacgt ggcgatggtc agcaaggaag
      481 tggatgcggt ggccggcgtc tcgctcgcgc aactcgcggc gcgtgaaggc gtggtctaca
      541 cgaccgccga cggcgaccag ccgtcgaatc tgatcggttg ggtgagctgg gcgcgcacgc
      601 ttggcctcga ggtcgtcgcg gccggcaaat cgagcgaata cgatctgatt ttcgatcccg
      661 ccacccatag cgtgacgcaa ctggacgaga cctttgccgc accggaactc gccgggctga
      721 tgaaactcgg cgacgacgtg cgcgccacgc tcgccgcacg ccgcgcggcg ttgaacggcc
      781 gcaagacctc ggcaaccgcc gactactgcg aaatggcggt ggtcgcgacc agcacggatc
      841 tgatacccga cgtcgaactg ctgcattacc cggttgcccg cacaaccgaa ctcgcggaca
      901 tctacgcgct tcaggaggac ggcggggtgc tcggcaagcg cggcgtgatc gacgtgttca
      961 acatgctgcg cctgccggac gaagcgagct tcgccggcgg cgtgttcgtg gtggtccgca
     1021 cgcacgacgc gccgacctgg gcgctgctgg cgcagaaggg ccatgtggtg agccgcaatg
     1081 gccgctacgc ctgcatgtat ctgccgtatc acttcatggg cgtcgaaacg ccgatctccc
     1141 tgctcgacgc cgtgctgcac ggccggccgt cgggggctga aacgccgcgg cagtgcacga
     1201 tcctcagcgg gcgcgcgagc gtcgatctgc cggccggcac cgtgctgaag atgggcggcc
     1261 atcatcacga cgtgacgggt gtgcaggccg tgctgatcgc acgcaacgac gcgccgcgcg
     1321 acatcgcccc gttgtatctg gccgcgcatg cgacgctcgc gcgcgacgtc aaggccggcg
     1381 tgttgctgag cgtcgacgac atcgcaggtt acgacgccga actgctgtca gcatggcaga
     1441 ccggtttcac gccggcctga gacggctcgc cgtccggtag aaaagcatga cgccaccggc
     1501 gcaagccatt cgccagcggt ggtgaacatc aaaacaagga gacggtatgc acggggacgc
     1561 agtaacgatc gtcatggcgg tcatcgccat tgcggtgctg gtggttctga ttgcgcggtt
     1621 caagctgagt ccgttcgtgg cgctcacgct gaccgcgatt ggtctcggta tcgtctcggg
     1681 cgtcacgcct gaacatacgg tcgacgcttt cgccaaaggg ttcggcggcg cgctggcgcg
     1741 taccgggccg gtggtcggcc tgggcgcgct gctcggcggc attctgatcg gcaccggcgg
     1801 cgccgaccgc atcgcgaatg cctttatcgg cacgcggccg atctactacg caccgtggac
     1861 ggtttgcgcc gcagccttgc tgatcggcat gccgcatctg ttcgacgtca gcttcatcat
     1921 gctggcgccg ctcgtctata cggtggccaa gcgcaccggc agccatctgc tgtacatcgg
     1981 tttgccactt gccgccggtc tctatgtatc gcatggtctg ttgccgccgc atccggcgcc
     2041 gatgctcgcg gtggtcgctt atcacgcgaa cacggggctc acgctcgcct atggtttgct
     2101 gattgcgatt ccgaccgcgg tgatctgcgg tccgcttttc actcgcgtga tacggcctta
     2161 tttcggtccg gcgccggacc tgaatacggg accggttgcg gcgagcgagc cggcggcgaa
     2221 tcgtccgcag gtgtcgctgg cgctctccgt gacgagcgtg ctgattccgc cgggactgat
     2281 gctgatcggt acgatgggga tggcgtacag cgtcaaggga agcctcgcct acatcctgtt
     2341 ccagtcggtc aacgatccga tcctctcgct gctggccgcg gtgattttcg cgttcttcgc
     2401 actgggcgtg cgcggcggct ttacgctggt cgacgtgcag aaaatggcga gcaaaggctt
     2461 gggcccgctc ggcgtgatcc tgctgattct cggcgcgggc ggcggtttca aggagatgct
     2521 cagcgcgatt catctcgacg tgatcatcgt gcaccacacc gcgggctggg ccgtcaatcc
     2581 gctcgtcctc gcctgggcca tcgcggcgtt gctgcgcatc tgcgtgggtt ccgcaacggt
     2641 ggcgacggtg gccgcgagcg gcatcgtcgc gccgctcctg ctctcgcatc cgggcgtctc
     2701 gcctgaactg atggtgctcg cgacggcctc gggcgcggtg atgctctcgc acgtcaacga
     2761 ctccggcttc tggctgttca aggagtattt ccaattgacc gtcgcacaga cgctgcgtac
     2821 gtggaccctg atgctgacgt tgcagtcgtt cgtggggctg gccggggttc tgctgctcga
     2881 gacagtcatc cgctaggcgc cgaggtcaat ccgttaagat gggactgcgc cgtgtcatcc
     2941 cgctcaggtg agcacggcgc gtcacagccc gcattcagga tctgccatgc ccagtcacac
     3001 cgaacccggc gcgtccgccg cgctcgacac cccactgatt ccggatcaac ggcgcgaatc
     3061 gatgttgcgc cagttgcgac gccacaaagt gttgagcgtc catcaactca ccgtcatgtt
     3121 cgactgctcg cacatgacgg tgcggcgcga tatcgccgtg ctcgaacagg ccgggctggt
     3181 ctattcggtt ccgggcgggg tacggattgc gagccagttg aacagcgagc cgagtcacca
     3241 gtcgaaagcg ctgatcgagc aaccgcagaa acaggccata gccgtgtgcg cggccgcgtt
     3301 cctgcgtgct ggcatgacgg cgtatctcga tgcgggcacg accacgctta cgctcgtgcc
     3361 gcacattctt gccttgagcg atatgacggt cgtcaccaac gactttccga tcgtgcagga
     3421 gctggccgag gcgacccatg tgaacgtgat ccacacgggc ggcatgctcg accacgcgaa
     3481 tcgctcgggc gtgggcggtc tcgcggccgc tacgctgcgc cacatcgcgg tcgacgtggc
     3541 gtttatctcc gccagctcgt gggatttgcg gcgcggggtg acaacgccgt ccgccccgaa
     3601 agtcgaagtc aaacaggcgg ccatggcggc tgcggcgcaa acggtattgc tcgcgaccag
     3661 ttccaaatac ggcacgttcg gcttgtacaa ggtcgccagg ctcgaacaat tcgaccacgt
     3721 gatcagcgac gaaggactga ctgaagccgc cgcggtgggg atccgcgaag ctggcgtcga
     3781 tctgacggtc gccaagggcg agtgagcgtg cggcgcagtt ttttccgcgc gtatggagtc
     3841 cgctcctgaa aatggagcag attgcgtgaa gcgggggaag gctttgcgtg gatccgtgct
     3901 tttgcgtttc gcgcaagacg agtcacaatt ggggcttcac cgtgaagcga ccccatggaa
     3961 aacctcctca agaaactcga ccttacctcg ctgcgcctgt tcgtcgccgt ctgccaggaa
     4021 cagaacatcg cgcgcgcggc ggagcgtgag ttcatcgcgt cttcggcggt gagccggcgc
     4081 attgccgaga tcgaggcggt gatcggcttg cccgtcattc agcgccagtc gcgcggcatt
     4141 accgtgacgc cggtcggcga gaccgtgctg cgctacgcgc tcgcgatcat cggcaacatc
     4201 gaacagatga gcgccgagtt gtcgcgcttt tcgtccggag cgaaggggcg ggtgcgggtg
     4261 gtggcgaatc tgtcgtcgat cgtgcagttt ctgccggaag atgtggccgc attcgggcgc
     4321 atgtttccgg aggtgtcgat cgagctggaa gaggagaaca gcacggacgt gctgcgtatc
     4381 gtcgacgaac acggcgccga cttcggcatc tgcaaccccg tagcgggcag cgaagccttc
     4441 gaacaggtgc cgtatcggga agaccggctg gcggtgctgg tgccgggcgg tcatcgtctt
     4501 gctggtgttc ctcgcgtggc gttcgaggaa ttgctcgacg agagcttcgt cggcctgcgc
     4561 agcgagagcg cgctgaccaa gctgctcgca cagcaggcgc tcagcgcggg acggcagctt
     4621 gatgtgaaga ttcgcgtcag cagcctcgac gcgttgtgcc ggatggttca cgccgggctt
     4681 ggcatcgcga tcgtgccgga acaggtggga ctgctgtatg tgaatgcgct cgacgtgcgg
     4741 ttgctgtccc tgagcgatat ctgggccgtg cggcatctca tcatgatctt caagtcgcgt
     4801 gatcagctaa gcgcgagcac cgcggctttg gtgggctttc taggcagcaa gccttagcct
     4861 gagacgcctc gcatcattct ccgggcgaga cctgtgagac gcctcgcatc attcactggg
     4921 cgagacctgt gagacgcctc gcatcattct ccaggcgaga cgtctgcgtt gccggaccgg
     4981 cacttcggca tcgcgcacgc gcgccgtata gtgggcgcaa ggagaatgca tatgagcgat
     5041 acgattcgtc tggtcggccg cgtgctgtac ctgtcccagg acccggcggt gatcgaggca
     5101 caactcgcgg gtgaaaattt cacacgcgtg agcgccggcc cgctgcgcga taacgtctcc
     5161 accgacgaaa tcacgccggt caccgtcatg ctcacctatg acgaacgcct cggccagtat
     5221 ccctacgtcg gcttcaaggc gggcgagcgc atgccgatcg gccgcaacgc gatcaaggac
     5281 ggcggctttc aggtgaccgt ggcgggcaag cgctacggca agggttcgtc gcgtgaatcg
     5341 agcccgttgg cggagttgtc ggccggcatc cggttgatcg tcgccgagag tttcgagcgg
     5401 atctatcagc agaactgcga caacatcggc attctcacga ccactgattt ctccgtgctc
     5461 gaccgtctga tcgcgggtga ggcggtgccg atcgatgagt tcctcaaagg ccgcgatgca
     5521 ctcacgcagc agatcattcg cagcggcggg ctgcttgcgt acagcaagtt cgcggactgg
     5581 ccagcgccgc gtgtgcgcga gacggcaaat ctggcaccca cggcagcccc ggcaaacacc
     5641 acacacgcca caaacgcgac aaccgcgaca aacgcgacaa acgccacaaa cacccccgag
     5701 ccgaagacac tcgtcgagaa gatcatcgag cgtcatcttc atcccggcat tgaaagcgcg
     5761 cagcgcggcg acggcgtgtt catcgccgcc gactggcgct tcagccacga ctacttcacc
     5821 ggcatgtgcg cgcatctgat gcatcgcgcg
//
LOCUS       DATASH010000852         1921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2322051,
            whole genome shotgun sequence.
ACCESSION   DATASH010000852 DATASH010000000
VERSION     DATASH010000852.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2322051"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /locus_tag="VHV99_22790"
     CDS             complement(<1..826)
                     /locus_tag="VHV99_22790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018432339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX3638251.1"
                     /translation="MKIFITGASGFIGGSIAAHLVRAGHQVRGLIRKPEHSAELQRLG
                     IEPVIGTLDHRDLLIAEAKAADAVINAASSDHKGAVKALIDGLAGSGKPFLHTSGSSI
                     VGDASGGEAGEARIYHEDALPEPTADKAARVAIDQLVLKAAQQNMRSAVLCNTLIYGH
                     GAVPGSASVQLPRLVRQAQKSGVVRHVGSGGNIWSNVHVDDVAELYRLALEKTPAGTF
                     YFVESGEASFRDMSAAIARVMKLGEAQDWPLEEAQKEWGYEMASYGLGSNSRVRGER"
     gene            1222..>1921
                     /locus_tag="VHV99_22795"
     CDS             1222..>1921
                     /locus_tag="VHV99_22795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HEX3638252.1"
                     /translation="MMILKPGYLTLPQLRRIAREHIALQLDPASHAAIDACAKAVADI
                     AAKGEPAYGINTGFGRLASTHIPHDQLELLQRNLVLSHAVGVGEPMSRPVVRLLIALK
                     LSSLGRGHSGIRREVMEALITLYNADVLPVIPVKGSVGASGVLAPLAHMSATLLGVGE
                     VFARGERMPATEGLALVGLKPLTLQAKEGLALLNGTQASTALALYNMFAIEDLYRTAL
                     VAGALSVDAAAGSVK"
BASE COUNT          351 a          634 c          578 g          358 t
ORIGIN      
        1 cgcgctcgcc gcgcacgcgg ctgttcgaac cgagcccgta cgaagccatt tcgtaacccc
       61 attccttctg tgcttcctcg agcggccagt cttgcgcctc gcccagtttc atcacgcggg
      121 caatcgcggc gctcatgtcg cgaaacgacg cctcgccgct ttcgacgaaa tagaaggtgc
      181 ccgctggcgt cttctccagc gcgaggcgat agagttcggc gacgtcgtcg acatgcacgt
      241 tcgaccagat attgccgccg ctgccgacat gccgcaccac gccgctcttt tgcgcctggc
      301 gcacgagccg cggcaactgc acgcttgcgc tgcccggcac agcgccgtgg ccatagatca
      361 gcgtgttgca cagcaccgcc gagcgcatgt tctgctgcgc ggccttgagc acgagttgat
      421 cgatggcgac gcgcgcggct ttatccgcgg tcggctcggg cagggcgtcc tcgtgataga
      481 tgcgcgcttc gcccgcttca ccacccgagg catcgccgac gatgctcgag ccgctcgtat
      541 gcagaaacgg cttgccggaa ccggcgaggc cgtcgatcag cgctttcact gcacctttat
      601 ggtcgctgct cgcggcgttg atgaccgcgt cggcggcttt tgcttcggcg atcagcaggt
      661 cgcggtggtc gagcgtgccg atcacgggtt cgataccgag gcgttgcaac tcagcgctgt
      721 gctcgggctt gcggatcaaa ccgcgcacct gatggccggc acgcacgagg tgcgccgcga
      781 tcgaaccgcc gataaagccg cttgcgccag taatgaaaat cttcaagagg ttctccctag
      841 ggtggaaaca ccggaaacac gatgacatgg acgcagtatc cgacgtcttg attctcgcaa
      901 aaagcgtatt agtctcaaat gaatcttgat ttgaaatcaa aaatgaagac gtccgcctgc
      961 ggactccgcg gcccttcact attttttgtc tgcttgccgg cgtggaattt gaacgtgtat
     1021 atacaagtca aaggtcatga ccatcggcag atgcgcggag cgctccctcg cgccgatccc
     1081 cgccagcagc gatatgccgc caggcaggca tcccgcccta atggaaaccc ctggtccgga
     1141 acctgcaaaa accagttgta tatacaagct gacgccgcta gacttctctc aaattgtata
     1201 gacaagacag gacacaacat catgatgatt ctgaagcccg gttacctgac cctcccgcaa
     1261 ctgcgccgga tcgcgcgcga acacatcgcg ctgcaactcg atcccgccag ccacgccgcc
     1321 atcgacgcct gcgccaaagc cgtcgccgat atcgccgcca agggtgagcc ggcctatggc
     1381 atcaacacgg gtttcgggcg tctcgccagc acgcacatcc cgcacgacca gctcgagctg
     1441 ctgcaacgca atctggtgct gtcgcacgcg gttggcgtgg gtgaaccgat gtcgcgtccg
     1501 gtcgtgcgtc tgttgatcgc gctgaagctg tcgagcctcg gccgcggcca ctcgggcatc
     1561 cgccgcgaag tgatggaagc gctgatcacg ctgtacaacg ccgacgtgct gccggtgatt
     1621 ccggtcaagg gctcggtcgg cgcatcgggc gtcctcgcgc cgctcgcgca catgtcggcc
     1681 actttgctcg gcgtcggcga agtgttcgca aggggcgagc gcatgccggc caccgaaggc
     1741 ctcgcgctgg tcggcctgaa accgctgacg ttgcaggcga aggaaggtct ggcgctgctc
     1801 aacggcaccc aggcgtcgac ggcgctcgcg ctctacaaca tgttcgccat cgaagacctg
     1861 taccgcaccg ccctggtggc gggcgcgttg tccgtggacg cagccgcggg gtcggtcaag
     1921 c
//
LOCUS       DATASH010000853        20680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2716593,
            whole genome shotgun sequence.
ACCESSION   DATASH010000853 DATASH010000000
VERSION     DATASH010000853.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 20680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20680
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2716593"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            327..1013
                     /locus_tag="VHV99_22800"
     CDS             327..1013
                     /locus_tag="VHV99_22800"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006752465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX3638253.1"
                     /translation="MTGFDRQTISDTTAKMLLEVQAVHFNAEKPYIFTSGWASPVYID
                     CRKLISYPRVRRGLMEMAETTILRDVGYEQIDAVAGGETAGIPFAAWLSDRLMVPMQY
                     VRKKPKGFGRNAQIEGLLTEGQRVLLVEDLTTDSRSTINFINALRTAGATVNHCFVLF
                     HYNIFKESVSVLKDIDVDLHALATWWDVLRVAKANNYFDSKTLDEVEKFLHAPAEWSA
                     AHGGANATPQ"
     gene            complement(1115..1804)
                     /locus_tag="VHV99_22805"
     CDS             complement(1115..1804)
                     /locus_tag="VHV99_22805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3638254.1"
                     /translation="MQDPVRVVVADDHPVILFGAEQALLKFPGLQVVARARQSTELVK
                     VLQTVECDVLVTDLAMPGGQYGDGLPLIGYLRRNFPDLPIVVLTMLENAALLKRLSEL
                     GVTSVVNKSDDLSHIGLAVQHVSRNLEYMSPSVKASLDTLRMNAGGKNEEVMLSRREL
                     EVVRLFVSGMTIKEIAEQLNRSIKTISTQKNTAMRKLGIDRDSELFQYAQSNGLLNLS
                     SHSVDDTGTAS"
     gene            2070..2672
                     /locus_tag="VHV99_22810"
     CDS             2070..2672
                     /locus_tag="VHV99_22810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEX3638255.1"
                     /translation="MRSRCLVVSLALPFRRLPFLSCAMLGLALLAAHNPDGLAENAFT
                     LTSASFRAGGTVNTAQVFDQDDCKGGNRSPQLTWHGAPPGTRSFAVTVFDQDAPGRGW
                     WHWAVAAIPAAVDSLPENASSSGFLKKLGAVEARNDFDIDGYGGPCPPPGKPHRYVIT
                     VYALSSADLRLAQGRPALMFDHEISTATLGSARMVVNYGR"
     gene            2945..3517
                     /locus_tag="VHV99_22815"
     CDS             2945..3517
                     /locus_tag="VHV99_22815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEX3638256.1"
                     /translation="MSKIVVVYHSGYGHTKKVAEAVLAGTLEAGADAKLMPVGEIDDA
                     AWAELAAADAIIFGAPTYMGGPSADFKKFADASSKPWFSQAWKDKIAAGFTNSATMNG
                     DKFSTIQYFVTLAMQHSMIWAGTGMMPSNTKAATRNDLNYVGGFTGLLTQSPADASPE
                     EAPPAGDLETARMFGVRIAGVTARWNAAPR"
     gene            3808..4755
                     /gene="argC"
                     /locus_tag="VHV99_22820"
     CDS             3808..4755
                     /gene="argC"
                     /locus_tag="VHV99_22820"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006053040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEX3638257.1"
                     /translation="MSTKVFVDGQEGTTGLKIFEYLSQRADVEILRIEEAKRKDLEER
                     RRLINASDVTFLCLPDVASRESASLVENDRTVLIDASTAFRTSADWAYGLPELARSQR
                     ERLRTAKRIAVPGCHASAFVLAMRPLVEAGVVAPEFAAHAYSITGYSGGGKKMIADYE
                     AGGNDKLKSPRPYALGLTHKHLPEMAAHTGLKSAPVFTPIVGDFYKGLAVTTFFSPNQ
                     LAKKTTLQDVHALFAEYYAGEAFVHVAPFDAQANLDSGFFDVQANNDTNRVDLFVFGN
                     EERFVTVARLDNLGKGASGAAIQCMNLAIGAAEESGLKR"
     gene            5243..5518
                     /locus_tag="VHV99_22825"
                     /pseudo
     CDS             5243..5518
                     /locus_tag="VHV99_22825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006053039.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6085..6954)
                     /locus_tag="VHV99_22830"
     CDS             complement(6085..6954)
                     /locus_tag="VHV99_22830"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006052586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3638258.1"
                     /translation="MDIGFIGLGEMGAAMVANILKAGHQVRVWNRSPERAQPLADVGA
                     RVVATPAEAFAGDAVFSMLADDTALREVITASLLEHAPRGLIHVNMATISVALAEELA
                     TAHASRGVHYVAAPVMGRPDVAAAGKLTIVAGGPAESIDRVQPIFDAIGQKTWRIGSL
                     PQQANVMKLAANFMLGAAVETLGEAATLVTGHGLAMQDFLDVITSGLFPGPVYAGYGK
                     MIAEQRYEPALFKARLGLKDLRLALAAADAVTTPLPIASVVRDSLIEAMAHGDGEKDF
                     AVLGQVAARRAGR"
     gene            7049..7804
                     /locus_tag="VHV99_22835"
     CDS             7049..7804
                     /locus_tag="VHV99_22835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HEX3638259.1"
                     /translation="MVTATFRFYEELNDFLDRPLRRRAFSYACAPGATAKHMIEVLGV
                     PHTEVELILVNGESVGFNHLLSEGDRIAVYPKFEALDIQPLLRVREHPLRVVRFIADA
                     HLGGLAPLLRLAGFDTLYDNHYPDADIEALAAAQQRIVLTRDRELLKRRSITHGCYVR
                     TLRPHEQLREVFERLDLAGSAQPFRLCLMCNAPLRRIAKAEVGDRAPDGVLERHHQFV
                     TCDVCRRVFWEGTHWQRMRALMDSVAGAPERHA"
     gene            7943..8221
                     /locus_tag="VHV99_22840"
     CDS             7943..8221
                     /locus_tag="VHV99_22840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638260.1"
                     /translation="MAMKKTDLEKNKALKLNNAMKLSTADRFGKAAAAEPKLDRREQR
                     KLDQAQGLVAFACKLNGDLVTELKERAATHPEGMTGLLNEVIKRGLAG"
     gene            complement(8375..8587)
                     /locus_tag="VHV99_22845"
     CDS             complement(8375..8587)
                     /locus_tag="VHV99_22845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006052583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638261.1"
                     /translation="MKKFIVSLAAASIALVSVQAFAQASDAAAPAAAASTPKKHHIKK
                     LDTHGKRAHVSSAASAAGTNDKGTQN"
     gene            complement(8801..8977)
                     /locus_tag="VHV99_22850"
                     /pseudo
     CDS             complement(8801..8977)
                     /locus_tag="VHV99_22850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090795.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
     gene            9243..10172
                     /locus_tag="VHV99_22855"
     CDS             9243..10172
                     /locus_tag="VHV99_22855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018432577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX3638262.1"
                     /translation="MKPDTRRHLRANLLMLIAAMIWGSAFVAQRLSLDAIGPFLFTGL
                     RFLLGALVVLAMIVCARRAALAELSKREPGGARELFGAGVLLGLVLAASISLQQIGLQ
                     YTKVANAGFISSLYVVIVPLLGVLFRHRTGFGTWLGATLAALGMYFLSVNEHFSILYG
                     DWYQLAGALVISVQMMLVGRFALRHDPLMLALVQFVTCGLACLVVGLAIEPVSLVGIE
                     RAAPTILYGGALSVGIAYTIQVVAQKHATPSHAAVIFSMEGVFAALAGWLVLGETLSA
                     RALFGCTLMLAGLIACQVMPARRRASERSPHVS"
     gene            complement(10160..10642)
                     /locus_tag="VHV99_22860"
                     /pseudo
     CDS             complement(10160..10642)
                     /locus_tag="VHV99_22860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011694660.1"
                     /note="sigma 54-dependent; regulates genes involved in
                     forming a 2',3'-cyclic phosphodiester on RNA; incomplete;
                     partial in the middle of a contig; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-dependent transcriptional regulator"
     gene            complement(10867..11118)
                     /locus_tag="VHV99_22865"
     CDS             complement(10867..11118)
                     /locus_tag="VHV99_22865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018432576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638263.1"
                     /translation="MREASEQSLRFLVEKWLAPGPSVPVHVTEFSRTRLGGRRYVRVE
                     TSLEAGSRGLFFFRHDDGCWCVFPPTADTPKLFAYPRAA"
     gene            complement(11897..12589)
                     /locus_tag="VHV99_22870"
     CDS             complement(11897..12589)
                     /locus_tag="VHV99_22870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2242 domain-containing protein"
                     /protein_id="HEX3638264.1"
                     /translation="MLISFSQATATAAALLGLLTLAACSGATQPKFQQELFETGASPY
                     ARNFNSSTTDACEAARRALLSQGYLTTMTRTDTVDGTKNFQPTGDTHIAVEFHVVCTP
                     GEEASDTSIVYVNAVQNGFALKKSDTSASVGLSVLGSLSLPIRSNSDAMVKISSETIP
                     SGKFYDRFFGLVDHYLQTVVRTQPVANSRIETRMLPVPVETTIPTPVPEMPDAIDKAA
                     SLQPASGAGVAP"
     gene            complement(12770..13411)
                     /locus_tag="VHV99_22875"
     CDS             complement(12770..13411)
                     /locus_tag="VHV99_22875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018432573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX3638265.1"
                     /translation="MSRLLCLIVLSLGLMQAATADENNDRMSAYLTQKFGLAKEQAQK
                     ISDAVQSAASKYSLPPSLLLAIISIESRFKEKAKGANGATGLMQVVPGAHRGLLRNVK
                     DLTEPTTNIEVGSAILYGYMRSANGDMNAALKSYGGSQAYAKKVSLRVEDFAGVAGPQ
                     DVASRPDAQASVCEADRCAAPDNWADAFTIPAVNGAAVKGAAALPNASPATPR"
     gene            complement(13818..14783)
                     /locus_tag="VHV99_22880"
     CDS             complement(13818..14783)
                     /locus_tag="VHV99_22880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HEX3638266.1"
                     /translation="MRASRLLSILMTLQARGRVTAQSLADECAVSLRTIYRDIDALGA
                     AGVPIQSERGAEGGYRLLDGYRTRLNGLSSQEAEALFLAGLPGPVQALGLRAVMAGAQ
                     IKLLAALPVELRSTAERMRSRFHLDAPAWFSDADQPANLPLIASAVWEQHPLEIRYQS
                     WKAEKFRRIEPLGIVLKSGAWYVVGRVGTDIRIYRISRILELSMLNETFERPAAFDLA
                     AYWQDSTQRLSEEMHASEATLRLSPWGMQMLEVFVSPFARSAAKIGEPDPADGWRTVT
                     LPVGSVRQACAELLRFGTEADVLAPPELRARFAEVATALHRRYAQ"
     gene            14907..15557
                     /locus_tag="VHV99_22885"
     CDS             14907..15557
                     /locus_tag="VHV99_22885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX3638267.1"
                     /translation="MTPGRTITLFHSPQCRSVSALTLLEELGSPYQLKVLNMKAGEQR
                     KAPYLAINPLGKVPAILHGDALVTEQVAIFIYLADLFPDAGLAPALDDPSRGPYLRWL
                     VYYAACYEPALVDKAMKRDLAPLATSPYGDFDSMLGTLTSQLEATPYLLGDTMSAADI
                     LWGIALHWGMMFKLVPETPVVLGYAQRICSRPSFVKVSERDVALAAEHEAAVKAAG"
     gene            15757..16017
                     /locus_tag="VHV99_22890"
     CDS             15757..16017
                     /locus_tag="VHV99_22890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6723 family protein"
                     /protein_id="HEX3638268.1"
                     /translation="MSKTAFIPSVPATSEEDFEVSATSKLAGYRRFFGVLKVVRTTDG
                     RVLFPFDGAPELGPYATKLEAVAAAQVYGEHIVTSDLARPEL"
     gene            16175..16711
                     /locus_tag="VHV99_22895"
     CDS             16175..16711
                     /locus_tag="VHV99_22895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638269.1"
                     /translation="MAACCRSGFAQNADAEADACQKALGEADVIHAQVHIAAIYPATN
                     SVTLRGPHGNLADVDVNPKLGDVRKLRVGDKLNVAYQQALLLQIDTVRTKGVRERIET
                     TATIPASAGYASSAHRVQIVATVLKIDRKSRMVTLRGPKHRQVLRAAERIPLYELKVG
                     DSVRAEFISAAAVEVVRE"
     gene            16911..17195
                     /locus_tag="VHV99_22900"
     CDS             16911..17195
                     /locus_tag="VHV99_22900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016347703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638270.1"
                     /translation="MLDRTEFLLATRQLAAAAELIAKAGPENLQIDALQMLALFRKHE
                     YLSRDSSAVATNDDELFARTGQAALTMAGRKEFAASHALLRQARSLLPST"
     gene            complement(17206..17574)
                     /locus_tag="VHV99_22905"
     CDS             complement(17206..17574)
                     /locus_tag="VHV99_22905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015877532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638271.1"
                     /translation="MTDPGFIEHESAHSDLLCFLVATAAASYALTNEWRVDHVVACCR
                     QWLKKNRLKMRWPDRVRIGQLALKIAGNDLLAAGVAVRLSSVNALFTSEMELNEASTM
                     VQRMKALCHDALAAHHGSSV"
     gene            complement(17695..19512)
                     /gene="glmS"
                     /locus_tag="VHV99_22910"
     CDS             complement(17695..19512)
                     /gene="glmS"
                     /locus_tag="VHV99_22910"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX3638272.1"
                     /translation="MCGIVGAVAQRNIVPVLIEGLRRLEYRGYDSCGVAVLDNDGPRR
                     ARSVARVADLDEQVRESHLEGITGIAHTRWATHGAPVTDNAHPIFSKDELALVHNGII
                     ENYESLREMLRGKGYTFVSQTDTEVIAHLIHSLYRGDLFAAVREAVAQLHGAYAIAVL
                     HKDQPHTVVGARQGSPLVVGLGNGENFLASDALALAGSTERFIFLEEGDVCELSLESV
                     RIADRDGNEAQRDVRQVAAYGGAVELGPYRHFMQKEIFEQPRAITDTIPQGDSFDASI
                     FGEGADKVFADIDNLLILACGTSYYSGLTAKYWLESIAKIPTQVEIASEYRYRESVPN
                     PKSLVVVISQSGETADTLAALKHAQSLGHKHTLAVCNVGTSAMVRQTELSFLTHAGRE
                     IGVASTKAFTTQLVALFVLAATLGKLRGQVSAEQEAEYLKQLRHLPAALNSVLALEPQ
                     IIAWSEEFSRKEHALFLGRGLHYPIALEGALKLKEISYIHAEAYPAGELKHGPLALVT
                     EAMPVVTVAPNDALLEKLKSNIQEVRARGGELYVFADADTKIVNDEGLHVIRMPEHYG
                     LLSPILHVVPLQLLAYHTACARGTDVDKPRNLAKSVTVE"
     gene            complement(19573..>20680)
                     /gene="glmU"
                     /locus_tag="VHV99_22915"
     CDS             complement(19573..>20680)
                     /gene="glmU"
                     /locus_tag="VHV99_22915"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011353430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEX3638273.1"
                     /translation="PLLDPSAPTLVLYGDVPLTRASTLQALTDRAGQGGYGVLTVALA
                     DPSGYGRIVRDEQGKVARIVEQKDANAEQLLIAEINTGIIVAPTERLAGWLAALKNDN
                     AQGEFYLTDAVEMAIEAGLEVVTTQPDEEWETLGVNSKQQLAELERIYQHNVAGALLI
                     AGVTLADPARLDVRGTLECGRDVSIDVNCVFEGHVTLADNVTVGPNCVIRNATIGAGT
                     RVDAFTHIEGAEVGANVVLGPYARLRPGASLQDESHVGNFVEVKNAVLGHGSKANHLT
                     YIGDADIGARVNIGAGTITCNYDGANKSRTIIEDDVFVGSDTQLVAPVRVKRGVTIAA
                     GTTVWKDVEEDMLVLNDKSQTSKPGYVRPTRKKS"
BASE COUNT         4060 a         6429 c         6192 g         3999 t
ORIGIN      
        1 caagccactc acaagcaagc caaaaagcag actggcaagc cgcgcgggaa gcaatcgcaa
       61 tccgacgtcg ggcagagacc ctgcaagcct cgttcagcgg tgccgtgaca acggtcgttc
      121 gcctccgatg cccacgacgg actcctcggg aaggccattt cgggtaaaat gtcggcctac
      181 gcgcgtagca aaaaccggcc cgtactccac tgaccggttg tgtatttgcc taccaaggcg
      241 ccgcccatca tggcgcggca taccccgctt gctgcgccac cgggccccgc gcccgcttct
      301 cctcgctcac acccaaggat ccgcccatga caggcttcga tcgccagacg atctccgaca
      361 ctaccgccaa gatgctgctg gaagtgcaag cagtgcactt caacgcggag aaaccgtaca
      421 ttttcacgtc cggctgggcg agcccggttt atatcgactg ccgcaagctc atctcgtatc
      481 cgcgcgtgcg ccgtggcctg atggaaatgg cggaaaccac catcctgcgc gacgtcggct
      541 atgaacagat cgacgcggtg gccggtggcg aaaccgccgg catcccgttc gcggcatggc
      601 tgtccgaccg cctgatggtg cccatgcaat atgtgcgcaa gaagccgaag ggtttcgggc
      661 gtaacgcgca gatcgaaggt ctgctgaccg aagggcaacg cgtgctgctg gtcgaagatc
      721 tgacaacaga tagccgcagc acgatcaact tcatcaatgc gctgcgcacc gccggcgcca
      781 cggtgaacca ctgcttcgtg ctgttccact acaacatctt caaggaaagc gtgtcggtgc
      841 tgaaagacat cgacgtcgat ctgcacgcgc ttgccacgtg gtgggacgtg ttgcgcgtcg
      901 ccaaggcaaa caactacttc gacagcaaga cgctcgacga agtggaaaaa ttcctgcacg
      961 cgccggccga atggtctgcg gcacacggcg gcgccaacgc aacgccgcag taagccaagc
     1021 cgctcaatgc ggatggaata aaaaaggcca cctgtcgatg agacaggtgg ccttttttca
     1081 ttgctcgtcc agccggacga aggtacgacc gcggttagga cgcggtgccg gtatcgtcga
     1141 ccgaatgcga cgacagattc agcaagccat tgctctgcgc gtactggaac aactcggaat
     1201 cgcgatcgat gccgagcttg cgcattgccg tgttcttctg cgtgctgatc gtcttgatgc
     1261 tgcggttaag ttgctccgcg atctccttga tcgtcatgcc ggacacgaac aggcgcacaa
     1321 cctcgagttc gcgacgggac agcatcactt cttcgttctt gccgccggca ttcatgcgca
     1381 gcgtatcgag cgacgcctta acggacgggc tcatatattc gaggttgcga ctgacgtgct
     1441 gcaccgcaag cccgatgtgg ctcagatcgt ccgacttgtt gacgaccgag gttacgccga
     1501 gttcgctcag ccgcttgagc agcgcggcgt tttccagcat ggtcagcacc acgatgggaa
     1561 ggtcgggaaa attgcgccgc agataaccga tcagcggcaa accgtcgccg tattgaccac
     1621 ctggcatggc cagatccgtg acgagtacat cgcactcgac ggtctgcaac accttgacca
     1681 gttcggttga ctgtcgcgcg cgcgcgacga cctgcagacc tggaaacttg agcaaagcct
     1741 gttcggcgcc gaacagaatc accggatggt cgtcagcgac cacgaccctg actggatctt
     1801 gcattgccct cccctcgaca gataaaccac tctccaaaac ttctgacatg cctttctatt
     1861 cttggtactt agactgatcg ggcacgcgtc tggtgttcgt gcggacaatt cctccgacta
     1921 tagccgattt cacgccggct cagcccgccg tcagcaacaa tgatccgcag cacagctaat
     1981 gttagacttg attcgacctc ggggcacaaa atagggacaa taatcttcgc gaggtcaact
     2041 gcctcgcgcc ctgacaggag aaccggcaca tgcgctcgcg ttgcctggtt gtttcgcttg
     2101 ctttgccgtt ccgccgtctg ccgttcctct cctgcgcgat gctgggtctc gcgctattgg
     2161 cggcgcataa ccctgacggg cttgccgaga acgcgttcac gctaaccagc gccagtttcc
     2221 gtgcgggcgg tactgtgaat accgctcagg ttttcgatca ggacgactgc aaaggcggta
     2281 atcgctcacc gcaattaacg tggcacggcg cgccgccagg cacgcgcagc tttgccgtca
     2341 cagtgttcga tcaggatgcg cccggccgcg gctggtggca ctgggccgtt gcggccattc
     2401 cggccgccgt cgacagcttg ccggagaatg ccagctcgtc cggcttcctg aagaaactcg
     2461 gcgcggtcga agcgcgcaac gacttcgata tcgacggcta cggtggcccc tgtccgccgc
     2521 caggcaaacc tcaccgctac gtcatcactg tctatgcact gagcagcgcg gatctgcgcc
     2581 tcgcacaggg acgcccggca ctcatgttcg atcacgaaat cagtaccgcc acactcggca
     2641 gcgcgcgaat ggtggtcaac tacggacggt agcggcgtcc gtcaaaacct gtctcgaacg
     2701 atatcggctg cgtgcctgag cgcgactcac accacgcctt gtgccgagcc gctgcgcatt
     2761 ccgccggctc gacacgcaag ttggcgcacg gcttgcgtta catcgcgtcc gcagcggaat
     2821 tgctgtcatc cgcgcacgct aatgtgcaac agcggggcgt gggcgatgct tgtcgatgtc
     2881 gcgtcgccgt gtgcgcactg ttgccgtgct taaccctgct tttttaatct cgccggagaa
     2941 tgccatgtcg aagatcgtcg tcgtttatca cagcggctac ggccacacga agaaagtcgc
     3001 tgaggccgtg ctggccggca cgctcgaagc aggcgcggac gcgaagctca tgccggtcgg
     3061 cgaaatcgac gacgcggcct gggcagaact ggcggctgcc gacgccatca tcttcggcgc
     3121 tccaacatac atgggcggcc catccgccga cttcaagaaa ttcgccgacg cgagttcgaa
     3181 accgtggttc agccaggcgt ggaaggacaa gatcgcagcc ggcttcacca attcggcgac
     3241 catgaacggc gacaagttct cgacgattca atacttcgtc acactggcga tgcaacacag
     3301 catgatctgg gccggcaccg gcatgatgcc gtcgaatacg aaggcggcaa cccgcaacga
     3361 cctgaactac gtgggcggct ttaccggcct gctgacgcag tcccccgcgg atgcgtcgcc
     3421 ggaagaagcg ccgcccgcgg gcgatctgga aacggcgcgc atgttcggcg tgcgtatcgc
     3481 cggcgtgacg gcgcgctgga acgcggcgcc tcgataacgc gctgtccgag ggttcgcgtc
     3541 ggacagcggc cacggccctg ccgcgtgcgc tcaaagcaga cggcgcacac ttcgcgatgc
     3601 gcggcgtagc tcttgccaag cgcagctaat ctatgcggaa cccactcgca tcggctcgca
     3661 tctgtcgcgt aacaggtcgt gccaatgtca ctttcggcgc gcatcgtcgt tttttcaccc
     3721 tgttttgcgc gcccgccgcc gtcaggtctt aaaatggcgt tccggcgcgg tgtcgcgccc
     3781 cgtttccagt aagtcagtga gatcgagatg agcacgaaag tttttgtcga cggacaggaa
     3841 ggcacgaccg gcctgaagat tttcgaatac ctgtcgcaac gcgccgacgt agagatcctg
     3901 cgtatcgaag aagcgaagcg caaggacctc gaggagcgcc gtcgtctcat caatgcatcg
     3961 gacgtcacgt tcctgtgcct gccggacgtc gcctcgcgcg agtcggcctc gctggtcgag
     4021 aacgatcgca ccgtgctgat cgatgccagc accgccttcc gcacgtccgc cgactgggcc
     4081 tacggcctgc ccgaactggc gcgctcgcaa cgcgagcgtc tgcgcaccgc gaaacgcatc
     4141 gccgtgccgg gctgccatgc gtcggccttc gtgctcgcca tgcgtccgct cgtcgaagcg
     4201 ggggtcgtag cgcccgagtt cgcggcgcac gcttattcga tcaccggtta tagcggcggc
     4261 ggcaagaaga tgattgccga ctacgaagcc ggcggcaatg acaagctcaa gagcccgcgc
     4321 ccgtacgcac ttggcctcac gcacaagcat ctgccggaaa tggcggcgca cacgggcctg
     4381 aaatcggcgc cggtgttcac gccgatcgtc ggcgacttct acaagggcct cgcggtcacc
     4441 acgttctttt cgccgaatca gctggccaag aaaaccacgc tgcaagacgt acacgcactg
     4501 ttcgccgagt actacgcagg cgaggccttc gtgcacgtcg cgccgttcga tgcgcaagcg
     4561 aatctcgaca gcggcttctt cgacgtgcaa gcgaacaacg acaccaaccg tgtcgacctg
     4621 ttcgtattcg gcaacgaaga gcgcttcgtg accgttgcgc gcctcgacaa cctgggcaag
     4681 ggtgcatcgg gcgccgcgat ccagtgcatg aatctcgcaa tcggcgccgc cgaagaaagc
     4741 ggcttgaaac gctgagccag ttccagacac tgacggtaat tcgccgtcaa tccgccgaaa
     4801 gccggtctgc aaagaccggc tttttgtttt aaaagccagt catcagaatt cccttattgc
     4861 gcttaatcag tagtagcccg cattacttaa aaaatagaaa tgggaccgct cattaaccga
     4921 cggtaattca ttccttaaat caacccgccc agtatagttt tccgcctagg gaaactccgc
     4981 attttttcgc gaacggtcgt tcgaagatga tagagccatt ttgccgcaac ccttgcctgg
     5041 caagggtgtt cggcgaagca acgatgccgc tgccaagccc aaaattcttt ttttgtttaa
     5101 aaggcctccc cagtcccaca tggcgtgcgc gcggcaaaaa tcgccctgtt tgaatcagtc
     5161 tttttagacg gctcattcaa ttgacaggca ttaaacgcac agcgaaatta gctcccacaa
     5221 atacatgaag ggagacagca gcatgtcgca cgccattaca agaggcttgg caatggccat
     5281 tgccacggcc ccattcctca tcgcttgcgg cggcggcaac gccgataatc caggcacgat
     5341 cagctcgcca caatgctcgg gatcgagctg cggcgtgcaa ggtccgccgc ccacaggctc
     5401 gaatacgagc gcgctgtgtc ccgctaccgc cgacattgtg aagaccacgt tcctgggcgg
     5461 agcaggtagc ggcgaagtgg tacgggtcaa catcgacgcg acggcgatga cctacacgcc
     5521 ggactggtcg gcgtccagca agcgaacggc cagaccggtc tcgcaatcgc ctcaggcggc
     5581 ctgtacgcga tcgagatcca gggcacggtc aataacgata tcacgccgac gtcggccaat
     5641 gcggacacgc cgaacgcccc ctacttcggc atcggtgcgc agatcagcaa gtaaccagcc
     5701 aggaaaacga cgcaacagca gttcgcacca caggacatag atagaacaaa tggtggccgg
     5761 gctgggagcc ggccacacac aacgagaaaa ttctggaggc ggtatgaaat ggagaatcct
     5821 ttcggtgctc gctcttgctc gatgactgtg cgaagccgcc ggcacgtgaa cgggaggacg
     5881 ccggaaactc aggcaaaaaa aagcccgcct aagttggcgg gctgaatcca tatcagagga
     5941 gacatggagg agacaagaag aactatagca aatgctttgg tgcgtcgcaa catacaaatt
     6001 agggttgacc ctcgccgttg tataaatgca acaatttttg cgcggcgttt ttgcaaacga
     6061 agccggccgt agccggccgc gagctcagcg ccccgcccgc cgcgccgcca cctgtcccag
     6121 cacagcaaaa tctttctcgc cgtcgccatg cgccattgct tcgatcaggc tgtcgcgcac
     6181 cacgcttgcg atcggcaacg gcgtcgtgac cgcgtcagcg gccgccagcg cgagacgcag
     6241 gtccttcaat ccaagacgcg ccttgaacag cgccggctcg taacgctgct ccgcgatcat
     6301 cttgccgtag cccgcataaa ctggccccgg aaacaggccg ctcgtgatga cgtcgaggaa
     6361 gtcctgcatc gcgaggccgt ggccggtcac cagtgtggcc gcctcgccaa gcgtctcgac
     6421 ggccgcgccc agcatgaagt tggccgcgag cttcatcacg ttcgcctgtt gcggcagcga
     6481 accgatacgc cacgttttct ggccgatggc gtcgaaaatc ggctgtaccc gatcgatcga
     6541 ctccgccggc ccgcccgcca cgatcgtcag cttgccagca gcggcgacgt cgggccgccc
     6601 catcaccggt gcggcgacat agtgaacgcc gcgcgaggca tgcgccgtcg ccagctcctc
     6661 ggccagcgcc accgagattg tcgccatgtt cacgtggatc aagccgcgcg gggcgtgttc
     6721 cagcagcgac gcggtgatga cttcgcgcag cgcggtatcg tcggcgagca tcgagaacac
     6781 ggcgtcgccc gcaaacgctt cagccggcgt cgccacgacc cgcgcgccga cgtcggcgag
     6841 cggctgcgcg cgttccggcg agcggttcca cacgcgcacc tgatgcccgg ctttcaaaat
     6901 gtttgcaacc atcgcggcac ccatctcgcc gagaccgata aaaccgatgt ccatctgtca
     6961 ctccgtcttc taaagaactg gaagtaaagc acacccatgc gacacgtgca ggacagcgcg
     7021 caatttaagc cggtgcgata ctgttgcgat ggtcaccgcg acattccgct tctacgagga
     7081 gctgaacgat tttctcgacc ggcccttgcg ccggcgcgcg ttcagctacg cctgcgcgcc
     7141 cggcgcaacc gcgaaacata tgatcgaagt acttggcgtg ccgcataccg aggtcgagtt
     7201 gatcctggtg aatggcgaat cggtcggttt caatcatctg ctgtccgagg gcgatcgcat
     7261 cgccgtttat ccgaagtttg aagcgctcga catccagccg ctgttgcgcg tacgcgaaca
     7321 tccgctgcgc gtggtgcgct tcattgccga tgcgcatctg ggtggactcg ccccgctgct
     7381 gcggctggcc ggcttcgaca cactctacga caaccactat cctgacgcgg acatcgaagc
     7441 gctcgcggcc gcacaacaac gcatcgtact gacgcgcgat cgtgagctat tgaaacgccg
     7501 ctccatcacg catggctgct acgtgcgtac gctgcgtccg catgagcaac tgcgcgaagt
     7561 gtttgaacgg ctcgatctgg caggcagcgc gcagccgttc cgtttgtgtc tgatgtgcaa
     7621 tgcgccgctg cggcgtatcg ccaaagctga agtcggtgat cgggcgcccg atggcgtgct
     7681 ggaacgacac caccagttcg tcacctgcga cgtgtgccgg cgcgtgttct gggaaggcac
     7741 gcactggcaa cggatgcgtg cgctgatgga cagcgtggcc ggcgcgccgg aacgtcacgc
     7801 ttgaacacct gctcgatgct gtagatgtcg cgcggcccgc actccgctcc aacgccgctc
     7861 gagtgcggaa tcgccgcggc tcgctcgacg cgcggcgcga tgcgtacaat gcgggatcac
     7921 ttatctggca gaggccttcc gtatggcgat gaaaaagacc gaccttgaaa aaaacaaggc
     7981 actcaagctg aacaacgcaa tgaaactgtc gaccgcagac cgcttcggca aagccgcagc
     8041 cgccgagccg aagctcgatc gccgcgaaca gcgcaagctc gaccaggcgc aaggcctcgt
     8101 cgcgttcgcc tgcaagctga atggcgacct ggtcactgaa ctgaaagagc gggccgccac
     8161 acatccggaa ggcatgaccg gactgctgaa cgaggtcatc aagcgcggcc tcgcgggcta
     8221 aacgccgcaa aaaccactgt tcaacgcaaa aaggccagag catgaactct ggcctttttg
     8281 ctggcgacat ttaacaggca ctacgttcca ctcgccggct agcggcgcgc agaagagaac
     8341 cggccggatt cgcccgacgc aaccaggcgt gagcttagtt ttgcgtgccc ttgtcgttcg
     8401 tgcctgcagc cgaagcagcc gacgaaacgt gagcgcgctt gccgtgcgtg tccagcttct
     8461 tgatgtggtg cttcttcggc gtgctagcag cagcggccgg cgctgctgca tccgatgcct
     8521 gtgcgaaagc ttgaacggag accagcgcga tcgaagcggc tgcgagcgag acgataaatt
     8581 ttttcatgtg ttgttcccca aacctggacg actgagtcaa tgtgaatgaa atagctgcac
     8641 ggtgaagcag acagggcttg aggcgcctta tcgctccccg cgggagagta ttacgcagtg
     8701 taggcggttt gtcatgccca aaactcgata tgtattttcc gggagacagc ttgctttcgt
     8761 gatgctttca ctcattagca gagcgtatca gggagattac ctagtagcgt ttgcagagca
     8821 gatttcgcag cggactggcg cgctcctgga gagtcggcag tacccgatgc agcgctgcat
     8881 tcgccgactc ctggccgatc ggcatgctca cgctgatcac cgccacgacc gcgccatgcc
     8941 ggtcacgcaa cggcaccacg acaccgcgca tgctcaaact tcgtcgaatg cttcgatgat
     9001 cgccagcgcc ttcgcggcgc cggcgaccca gtctttctcg tccattgcgt ctgcccgaaa
     9061 gtcggcctgc ccggtgggtc agtcgtgtct cgagccgtcc ggtcattgcg cgattatcgc
     9121 gcgagtggct cgccgtgcaa ccggctggat tgtggaggcg tatccggcgc gcgtgcgcgc
     9181 ggataaaatc ggcgccgggc cctgcacgcc ttgttagaac aaccgaattc gaaaaactgc
     9241 ctttgaaacc tgatacacgc cgacacctgc gcgccaatct gctaatgctg atcgcggcca
     9301 tgatctgggg ctccgcgttc gtcgcgcaac gcctgagcct cgatgcgatc ggaccgtttc
     9361 tgtttaccgg actgcgtttc ctgctcggcg cgctggtcgt gctggcaatg atcgtctgcg
     9421 cgcggcgcgc cgcgcttgcg gaattgtcga agcgcgaacc cggcggcgca cgcgagctgt
     9481 tcggtgcggg tgtgctactg ggcttggtgc tggctgcgtc gatttcattg caacagatcg
     9541 gcctgcaata cacgaaggtc gccaacgccg gcttcattag ttcgttgtac gtggtgatcg
     9601 tgccgctgct gggcgtgctg tttcggcatc gaaccggatt cggcacgtgg ctcggtgcga
     9661 cgctcgctgc gctcggtatg tactttctga gcgtcaacga gcacttctcg atcctgtatg
     9721 gcgactggta tcagcttgcc ggcgcgttag tgatttccgt acagatgatg ctggtcgggc
     9781 gcttcgcgct gcggcatgac ccactgatgc tggcgctcgt gcagttcgtg acttgtgggc
     9841 tcgcatgtct ggtcgtcggc ctcgcgatcg agccagtcag tctggtgggg atcgagcgtg
     9901 ccgcgccgac tattttgtat ggcggtgcgt tgtccgtggg catcgcttat acgattcagg
     9961 tggtcgcgca aaagcatgcg acgccgtccc atgccgccgt gatcttcagt atggaaggcg
    10021 ttttcgccgc gctcgccgga tggctcgtgc tcggcgaaac actatcggcg cgcgcgctat
    10081 tcggttgcac gctgatgctc gcgggtttga tcgcgtgtca ggtgatgccc gcccggcgac
    10141 gcgcaagcga gcgttcgcct cacgtctcgt gagggtttag ccaatcgccc cattccacgc
    10201 cgaaacgtgc gagatattta cgcaagcggt cggcatcgtt cggctgcttc ctggcttgcc
    10261 gcgataccgc aaatagcgtg cgccccgctt ccgacagact cgacgacgcc tcgcacacct
    10321 ccagcacacg ctgcagttgc aagcgatcga acaggtcgta ctcgtcgacg cgcgcgccga
    10381 gggtgcgtga cactatgtcg ccgtcggcat ctccgagcgg aggtgtccat gtccgctgca
    10441 gacgctcgac ctcctgaagg gcgttgtctt ccgtgatgcg cccggcgtcg gccaaagtcg
    10501 ccatccgcgt caccgaggcc gatacctcgc ggaaattgcc cgtccacgcg gccttcgctg
    10561 acgtggcgaa cgtcagataa cggcgccgtg cttcgacgtt gaaacgcgcc agcttgccct
    10621 gctcgcgccc gaagcgacgc agtgatcgca gcgcagtctc gtcgaggtgg tacacacgcg
    10681 gcggcaggtg gcgtgcgtga aggggtagcg cgggggcatc ggcaaagcgc gggtcgcccg
    10741 ttcagcgatc cgcgccgggg ggcgcgctgg atgactgctt acgcaaggcg attcgcgtaa
    10801 agcacataaa gcacacagtc atcatttccc gacggcgagg cggccgcttg acggcggcgt
    10861 ccgccgtcaa gcggcgcgcg gatacgcaaa cagcttgggg gtgtcggcag tgggtggaaa
    10921 cacacaccag cagccgtcgt catgacgaaa gaaaaaaaga ccgcgtgacc cggcttcgag
    10981 agaagtctcc acgcgcacat agcgccttcc gcccagacgg gtgcggctga attcggtgac
    11041 gtgaaccgga accgaaggac ccggcgccag ccatttctcc accaggaaac gcagcgactg
    11101 ctcgctagct tctctcatga tccgctccgg accggcttgt cgtgatgcgt gtcgcgtgcg
    11161 gcttcgttgc tttcgccgct cccggcgccc tcctcgtcat gctcggagga tgcgagcgat
    11221 atggcccggt tagtggctgc cgcccgcagc gtcgtgcagt ggctggcatg cctgagatcc
    11281 gagagaagac ggactagctg tcgtgctgtt ctgcgtttca tgactcaccc ccgcactgca
    11341 ccaataactt cagaaaacac tttaactcta tgcctggata gggcgttgct gaaatggttt
    11401 tgactaaata gcaacagttg cgcttgcaaa aacagagacg caatctttgt accttccgac
    11461 ttcaatgtgc gcgccttgag gagttccggt ctgtacggtg gcgagcggat ggcgtcgacg
    11521 aaaaagctga gccgccgcct ggcgcaatgc ggccggatac gcaagttatc cacaattacc
    11581 tatgaataac ttcgggatag cgcatcggac agcctgtgcg cggaatcttg ataaggcgcg
    11641 gcagcgggcc agccccgtca aagttatccc ggctttgcag tttttctaca gagatgttcc
    11701 gctgggttat ggttttcgca tcaccttgat gtaacggcga atttgagcgt tatccacaga
    11761 cggggttaac ccttgttaac tattactaca tatatatacg tatgaagtaa aagacatagg
    11821 ataagtagca cagcctgatg agatcacagg ctgatcaggt cctggtcgag ctgaatccgc
    11881 aacattagcg gatgcatcaa ggcgctacgc cggctccgct tgcaggttgc agagaagcag
    11941 ccttgtcgat cgcgtccggc atctcgggaa caggcgtggg aatcgtcgtt tcgaccggca
    12001 cgggcaacat gcgggtctca atgcggctgt ttgcaaccgg ctgcgtgcgc accacggttt
    12061 gtaaataatg atcgacgaga ccgaaaaagc ggtcgtagaa cttgcccgac gggatggtct
    12121 cgctcgaaat cttgaccatc gcatcgctgt tcgagcggat cggcagcgac agtgatccga
    12181 gcacgctcag tcccacactc gccgaggtat cgcttttctt cagcgcaaag ccgttctgca
    12241 cagcattgac gtaaacgatg ctcgtatcgc tcgcctcttc acccggcgta cacaccacat
    12301 gaaattcgac ggcgatgtgc gtatcgccgg tcggctggaa atttttcgtt ccgtctaccg
    12361 tatcggtgcg cgtcatcgtc gtcagatagc cctgactcag cagcgcgcgg cgcgcggcct
    12421 cgcaggcatc cgtggtgctt gagttgaaat ttcgtgcgta aggactcgcg cccgtttcga
    12481 acaattcctg ctggaatttc ggctgcgtgg cgccgctaca ggcggcaagt gtcagcaggc
    12541 cgagcagcgc agccgctgtc gcagtggcct gggaaaacga gataagcatg gtcgatcgga
    12601 agaggcgcgg cgtgagctcg aatgggaatg cattgtagag cggttgccaa ctgtgcgtaa
    12661 aaggcgcgca gcgcacgccc cgcggcgcct aacgcggcgt gacgactctt ctccggggct
    12721 tcagtgagaa agcgcgacag agactcaaca agaggaagga agcttcagtt cagcgcggcg
    12781 ttgcgggcga ggcgtttggt agcgccgcgg cgcccttcac tgcggcgcca ttgacggccg
    12841 gtatcgtgaa agcgtcggcc cagttatccg gcgccgcgca gcggtcggcc tcgcacacac
    12901 tggcttgcgc gtccggacgc gatgcgacgt cctgtggccc agcgacgcct gcgaagtctt
    12961 cgacgcgcaa gcttaccttc ttcgcatatg cctgcgatcc gccataactc ttgagcgcgg
    13021 cgttcatgtc gccgtttgct gagcgcatgt agccgtacag gatcgccgag ccgacttcga
    13081 tgttagtagt cggctccgtg aggtccttga cgttcctcag cagcccgcga tgggcgcccg
    13141 gcaccacctg catcagcccg gtcgcgccgt tggcgccctt ggccttttcc ttgaagcggg
    13201 attcgatcga gatgattgcc agcagcaggg acggcggtag tgaatatttc gaagcggcgg
    13261 actgcaccgc gtccgaaatc ttctgagctt gttcctttgc cagcccgaat ttctgcgtta
    13321 gatacgcgga catgcggtca ttgttttcgt ccgccgtcgc ggcttgcatc aggccgagcg
    13381 aaagcacgat caggcaaagt aaccggctca taacggagga cggggaaaga aaggaattga
    13441 cgcattatag ctctgccccc aaaaggggca ccaaaccacc gaaattcctc agaggaaagc
    13501 ccacgagcgg gtgatttctg aacgcttcac gtgtcgcgtt acacgtagag gcgcagcgcg
    13561 ttcttcagcg tgtgaaaaca gggcgttgcg gaagaacatt cgtttgcgcc caacgctggc
    13621 tttgcgcgac acgcggagcg cctgggaagt tgaagtggct gaggtagcgg acgagaaccc
    13681 tcgacgcaca atcgatcgat atagcaggag gcgcaatcgc cggccgccgg attgtcgaca
    13741 gttctttgct tctgaatcac gatccgttag ttgcaattca catcgaggct ccaatcgaag
    13801 ttgctgaacc agcgccctca ttgcgcgtag cggcgatgca aggcggtcgc cacttccgcg
    13861 aaacgggcgc gtaattccgg tggcgccagc acgtcggcct cggtgccgaa gcgcagcaac
    13921 tccgcgcatg cctgcctgac cgagcccacc ggcaatgtga ccgtgcgcca gccgtcggct
    13981 ggatccggtt caccgatttt cgccgctgag cgtgcgaacg gtgagacgaa gacttcgagc
    14041 atctgcatgc cccatggcga gaggcgcagc gttgcctcgc tcgcgtgcat ctcttcggac
    14101 agacgctgcg tgctgtcttg ccagtaagcc gccagatcga aagcggccgg gcgctcgaat
    14161 gtttcgttca gcatgctcaa ttcgagaatc cgcgaaatgc ggtagatgcg gatatccgtg
    14221 ccgacccggc caaccacata ccaggctccg cttttcagca cgatgccgag cggttcgatg
    14281 cggcgaaact tttcggcttt ccaactctga tagcgaatct ccagcggatg ctgttcccac
    14341 accgcgctgg cgatcaacgg caggttcgcg ggctgatccg cgtcggaaaa ccaggctggg
    14401 gcatcgaggt gaaagcgcga gcgcatgcgc tccgcggtcg agcgcaattc gaccggcagc
    14461 gcggcgagca acttgatttg cgcgccggcc atcacggcgc ggagaccgag tgcttgcacc
    14521 ggtcccggca agccggcgag gaacagcgcc tccgcttcct gcgaagagag accgttcaga
    14581 cgcgtgcggt atccatcgag cagacgataa ccgccctccg cgccacgttc gctctggatg
    14641 ggaacgcctg cggcgcccaa ggcgtcgatg tcgcgataga tcgtgcgcag cgaaacggcg
    14701 cattcatcgg caagcgactg cgcggtcaca cgtccacgcg cttgcagtgt catcaggatg
    14761 gaaaggaggc ggctggctct catgccgaga aagtgtagtc atacctgaca caaactgaca
    14821 ggtacgaagg cctagtatgg ctgcatccgg cgccagatga atgctggtgt tccccaccga
    14881 tgttcattga ccaggaggcc ccagccatga ccccaggacg cacgatcacg ctgttccact
    14941 cgccgcaatg ccgctctgtc agcgcactca cgctgctcga ggagctcggc tccccgtatc
    15001 agctgaaggt gttgaacatg aaggcaggcg agcagcgcaa ggcgccctat ctcgcgatca
    15061 atccgctcgg caaagtgccg gcgattcttc acggcgacgc gcttgtcacc gaacaagtcg
    15121 ccatttttat ctatctggcc gacctgtttc ctgacgcggg tctcgcgccg gcgctcgacg
    15181 atccatcgcg cgggccatat ctgcgctggc tggtgtacta cgcggcatgt tacgagccgg
    15241 cgctcgtcga caaggcgatg aagcgcgacc ttgcgccgct tgccacctcg ccttacggcg
    15301 acttcgattc gatgctcggc acgctgacga gccagttgga ggccacgcct tatctgctcg
    15361 gggacacgat gtcggcggcg gatatcttgt ggggcatcgc gttgcattgg gggatgatgt
    15421 tcaagctggt gcccgaaacg ccggtcgtac tcgggtatgc ccagcgtatc tgctcgcggc
    15481 cgagctttgt gaaggtgagc gagcgggacg tcgcgttagc ggccgagcat gaggctgcgg
    15541 tcaaggccgc cggatgacgc tgcttacggc gaggcctgtc gcggtgatgc aggacgcggg
    15601 gcgcacgcgg cggccggttc gctgatcggc ttccggttga atgcgccggg atttgagaca
    15661 atgaaggccg ccggtgaatg tcctccccgg acaaccgggt gtcacctccg cctttcatgc
    15721 agatgccgcc ccattgaaca agagatgttt tcaggtatga gcaagactgc gtttattccc
    15781 agcgtccccg cgacgtcgga ggaagacttt gaagtttcgg ccacgtcgaa gctggctggc
    15841 taccggcgtt ttttcggcgt gttgaaggtg gttcgcacca ccgacggccg cgtgctgttt
    15901 ccgttcgacg gcgccccgga gctcggccct tacgcgacca agctggaggc cgtcgcggcg
    15961 gcgcaggtat atggcgagca catcgtgacc agcgatctgg cgcgcccgga gttgtaagcg
    16021 agggagcgtg gctgaggtgt tgtgatcacc tggattgatc gcttggcttg gtcattcgga
    16081 ttgtcatttg aggtggtcac ttcgggagca atggatgtct cgtctgcgca aagtgggctc
    16141 atcggccgcg gttgtatgga cggcggttgc gctgatggcc gcgtgctgtc gatccggctt
    16201 cgctcagaac gctgacgctg aagccgatgc ctgtcagaag gcgctcggag aagccgatgt
    16261 cattcacgcc caggtccaca tcgcagcgat ctaccctgcc accaacagcg tcacgctacg
    16321 tggtccacac ggcaatctgg cggatgtcga cgtcaacccc aagctgggcg atgtcaggaa
    16381 actacgggtc ggcgacaagc tgaatgtcgc gtaccagcag gcgctgctgc ttcaaatcga
    16441 cacggtgagg accaaaggcg ttcgtgaacg catcgagacg acggcgacga taccggcttc
    16501 agcgggatac gcatcgtcgg cgcatcgggt gcagatagtc gcgacggtgc tgaagatcga
    16561 ccgcaaaagc cggatggtga cgctgcgtgg gccgaagcat cggcaggtgc tgagggcggc
    16621 agagcgcatc ccgctctatg agttgaaagt cggggatagc gtgcgggcgg agtttatctc
    16681 ggcggctgcg gtggaggtgg tgagggagtg aggtgtccaa gccgcaccaa tagccggcgc
    16741 aagttccgat atttcacttc gctcgaaaag cagacgttct aacgcgtcaa tacgttcatc
    16801 gtcgaataga agcgtgttaa taaggtcgtt cgaatcacaa atgaatgcat ccgaggcaaa
    16861 tacgagcaag cagggacctc tcccggaggc tcttgccata cgaggtcagt atgctggacc
    16921 gcactgaatt cctgttggcc acgcgacaac tggcagccgc tgctgagctt attgccaagg
    16981 ctggtcctga gaatttgcaa atcgatgcgc tgcagatgct agcgttgttt cgcaagcacg
    17041 aatatttgag cagagattcg agtgctgtcg cgacaaacga tgacgaactg tttgcgcgca
    17101 caggccaagc cgcactaaca atggctggac gcaaggagtt tgccgcgtcg catgcccttc
    17161 tgaggcaggc gcggtcactg ttgccctcga cgtgaaaagc agttcctaca cgctggaacc
    17221 gtgatgtgcc gcgagtgcgt cgtggcaaag cgccttcatc cgctgaacca ttgtgcttgc
    17281 ctcgttgagt tccatttcgc tggtgaagag ggcattgacg ctagacaacc ggaccgcgac
    17341 cccggccgcc agcaagtcgt taccggcgat cttcagagca agttggccga tgcgaactcg
    17401 gtccggccag cgcatcttca ggcgattttt cttcaaccac tggcggcagc acgccacaac
    17461 atggtccacc cgccattcgt tagtcagcgc gtaggatgct gctgcagtcg cgacgaggaa
    17521 acatagcaag tcggaatgcg cactttcgtg ctcaataaat ccagggtcag tcatgactat
    17581 tggtttgcct gctatggtta agcaaaccca ctacgtgtgg gcgagggcgt gtgaatcaat
    17641 atggtcacaa tttcacggta agtgcagtgt agcgtttcag cccgcacctc accctcactc
    17701 caccgtcacc gacttcgcca gattccgcgg cttatccaca tccgttccac gcgcacaagc
    17761 cgtgtggtat gccagcaatt gcagcggcac cacatgcaga atcggtgaca acaagccata
    17821 gtgctccggc atccggatca catgcagacc ttcatcattc acgatcttcg tatccgcgtc
    17881 cgcgaacaca tagagctcac cgccccgcgc gcgcacttcc tgaatattcg acttcagctt
    17941 ctccagcagc gcgtcattcg gcgccaccgt caccaccggc atcgcttcgg tcacgagcgc
    18001 cagcggccca tgcttcagct cgccagccgg atacgcttcc gcgtgaatat aggaaatctc
    18061 cttgagcttc aacgcgccct caagcgcgat cgggtaatgc aagccgcgac cgaggaacaa
    18121 cgcatgctcc ttgcgcgaaa actcttccga ccacgcaatg atctgcggct ccagcgccag
    18181 cacgctattc aacgccgccg gcaagtggcg cagttgcttc agatactcgg cttcctgctc
    18241 cgcgctcacc tgcccgcgca gcttcccaag cgtcgcggcc agcacgaaca gggcaaccag
    18301 ttgcgtcgtg aacgccttgg tcgatgccac gccgatctcg cggcccgcgt gcgtcaggaa
    18361 cgacaactcg gtttggcgca ccatcgcgct cgtgccgacg ttgcacaccg ccagcgtatg
    18421 tttgtgcccc agtgactgcg catgtttgag cgccgccagc gtgtcggccg tttcgcccga
    18481 ctgcgaaatc accactacca gcgacttcgg attcggcacc gactcacggt accgatactc
    18541 gctggcaatt tccacctggg tcgggatctt cgcaatcgac tcgagccagt acttcgcggt
    18601 cagtcccgag tagtagctcg tgccacatgc cagaatcagc aggttgtcga tatccgcgaa
    18661 caccttgtcg gcaccttcgc cgaatatcga cgcgtcgaac gaatcgcctt gggggatcgt
    18721 gtcggtaatc gcgcgcggct gctcgaagat ttccttctgc atgaagtgac gatacgggcc
    18781 gagttcgacc gcgccgccat acgccgcgac ctggcgcacg tcgcgctgcg cttcgttacc
    18841 gtcacgatcg gcgatacgca cgctttcgag cgacagttcg caaacgtcgc cttcttcaag
    18901 gaagatgaag cgctcagtac tgccggcaag cgccagcgca tccgaagcaa ggaagttctc
    18961 gccgttgccg agacccacca ccagcggcga accttgccga gcgccgacca ccgtatgcgg
    19021 ctgatccttg tgcagcaccg cgatcgcata cgcgccgtgc aattgcgcga ccgcttcacg
    19081 caccgccgcg aacaggtcac cgcgatacag gctgtgaatc agatgcgcga taacctccgt
    19141 atcggtctgc gagacaaacg tgtaaccctt gcctcgcagc atttcacgca gagactcgta
    19201 gttttcgatg atgccgttgt gtaccaacgc gagctcatct ttcgagaaga tcgggtgcgc
    19261 gttgtcggtc accggcgcgc cgtgcgtcgc ccagcgcgta tgcgcgatac ccgtaatgcc
    19321 ttcaaggtgg ctttcgcgca cctgctcgtc gagatccgcc acacgcgcga cgctgcgagc
    19381 acggcgcggc ccgtcattat cgagtaccgc gacgccgcac gaatcgtagc cgcgatactc
    19441 gaggcgacgc agtccttcga tcaggacggg aacgatatta cgttgcgcaa ccgcgccgac
    19501 aatcccacac atggcttatt ccttttcagt gccgggtttc agggagcgcg ctccgcgcgc
    19561 gcgtcacgcc catcaacttt tcttcctggt cgggcgtacg tatcccggct tgcttgtttg
    19621 agacttatcg ttcaggacca gcatgtcttc ttcgacatcc ttccagaccg tcgtacccgc
    19681 ggcgatcgtc acgccgcgct tcacgcgcac tggcgctacg agctgcgtat ccgaaccgac
    19741 gaacacgtcg tcttcgatga tcgtgcggga cttgttcgcg ccatcgtaat tacaggtgat
    19801 ggtacccgcg ccgatattca cgcgcgcgcc gatatccgcg tcgccgatat acgtcagatg
    19861 attcgccttc gagccatgac cgagcaccgc gtttttcacc tcgacgaagt tgcccacgtg
    19921 cgactcgtcc tgcaacgacg cgcccggacg cagccgcgca taggggccga gcaccacgtt
    19981 cgcaccgacc tcggcgcctt cgatgtgcgt gaaagcgtcg acgcgcgtgc cggcaccgat
    20041 cgtcgcgttg cggatcacgc agttcggacc cacagtcacg ttgtcggcca gcgtgacgtg
    20101 gccttcgaac acacagttca cgtcgatcga gacgtcgcgg ccgcattcga gtgtgccgcg
    20161 cacgtcgagg cgagccgggt ccgccagggt gacgccggca atcagcagtg ccccggcgac
    20221 attgtgctgg tagatccgtt cgagttcggc aagctgctgc ttgctattca caccgagcgt
    20281 ttcccactct tcgtccggtt gggtggtgac gacttcgagc ccggcttcga tcgccatctc
    20341 gaccgcgtcg gtcagataga actcgccttg cgcattgtcg tttttcaatg ccgcgagcca
    20401 gccggcgagt cgctcggtcg gcgcgacgat gatgccggtg ttgatctcgg caatcagcaa
    20461 ttgctcggcg ttcgcgtctt tctgttcgac gatgcgcgcc accttgcctt gctcgtcgcg
    20521 cacgatgcgg ccgtagccac tcgggtcggc gagcgcgacg gtgagcacgc cgtagccgcc
    20581 ctgccccgcg cgatcggtca gcgcctgcag cgtgctggcg cgcgtgagcg gcacgtcgcc
    20641 gtaaagcacc agcgtgggcg cggacggatc gagcagcggc
//
LOCUS       DATASH010000854         3570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1135157,
            whole genome shotgun sequence.
ACCESSION   DATASH010000854 DATASH010000000
VERSION     DATASH010000854.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1135157"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="VHV99_22920"
     CDS             <1..533
                     /locus_tag="VHV99_22920"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEX3638274.1"
                     /translation="QHLKVGDEVLIGKKPVGTLMADNLLPGKTLWLLSTGTGLAPFMS
                     IIKDPDIYDRYERVVLTHTCRFVDELAYKEYITDHLPAHEHLGELVQEKLLYYPTVTR
                     EAFQNRGRITELIETEKLFADLGVPGFSLEDDRVMLCGSPHMLRDTRKLLDDLGFQEG
                     SNNAPGHYVVEKAFVG"
     gene            968..1819
                     /locus_tag="VHV99_22925"
     CDS             968..1819
                     /locus_tag="VHV99_22925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3638275.1"
                     /translation="MDTSTVVPFSNHNPPTPAYTAPQSAAERELAQLRARVRELSAEL
                     VQAQEAACRHVARELHDGVGAELTATRFALAGVETWLPADAPPQCSAALAVANRSLDA
                     VCAASRQAVAELHAPSLETGIVGALSQWTGDFAARTNLRTSFVCAADVRLTRLPADAA
                     LAVFRVAQEALNNIARHARAESADVRIEAGRRHLTLIVSDDGVGMTRNARSRRGHFGL
                     SGMQARCAAFDGTLRITARRGASSRGDSGKAPRGTTVRARFAWDAMLALAPGARRRAL
                     QSCSTPS"
     gene            1816..2472
                     /gene="rqpR"
                     /locus_tag="VHV99_22930"
     CDS             1816..2472
                     /gene="rqpR"
                     /locus_tag="VHV99_22930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050491.1"
                     /note="The RqpSR system (Regulating Quorum sensing and
                     Pathogenicity Sensor kinase and Response regulator)
                     co-occurs with and modulates the expression of
                     cis-2-dodecenoic acid quorum-sensing systems; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RqpR"
                     /protein_id="HEX3638276.1"
                     /translation="MSLRILLVDDHAVVRQGVRQLLLDRGVAREVTEAQSGAEALDAV
                     AQHSYDVVLLDISLPDMNGVEVLKRLKRKAPRVAVLMFSMYREDQYAVRALKAGAVGY
                     LSKTVDAAQMIGAIQQVAAGRKYVSPAMAEALADYVSFDGEQLPHEKLSDREYQTLCM
                     LASGKRLTDIAMALSLSVKTVSVYRTRLLEKMKLRNNAELTFYVMSNRLVDLNPAMAG
                     "
     gene            complement(2579..2773)
                     /locus_tag="VHV99_22935"
     CDS             complement(2579..2773)
                     /locus_tag="VHV99_22935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638277.1"
                     /translation="MDFTSAPFWDGVFTVAGQREQVWLKPSGRGVLPEHEKRAQSLFD
                     LDLRARIELRRRESDSYPRD"
     gene            2828..>3570
                     /locus_tag="VHV99_22940"
     CDS             2828..>3570
                     /locus_tag="VHV99_22940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX3638278.1"
                     /translation="MSLFRKKSVEHMIATSAQNAGLKKALGALDLTFLGVGAIIGTGI
                     FVLTGTGAVQAGPALMVSFLIAAVACGFAALAYAEFASTIPVAGSIYTYSYATLGELA
                     AWIIGWDLMLEYGLATSAVSVGWSGYLQSLLSGFGVSLPVALTAAPGALPGHETLFNL
                     PAFLVMMAITALLSLGVRESARINNIMVAIKVTVVLLVIGVGVFHVTPANWHPFMPNG
                     WNGVFGAAAVMFFAFIGFDSVSSAAEEVK"
BASE COUNT          636 a         1139 c         1112 g          683 t
ORIGIN      
        1 tgcagcatct gaaggtcggc gacgaagtcc tgatcggcaa gaagccggtc ggcacgctga
       61 tggccgacaa cctgctgccc ggcaagacgc tgtggctgct gtccaccggc acgggtctcg
      121 cgccgttcat gtccatcatc aaagatccgg acatctacga ccgctatgag cgcgtggtcc
      181 tgacgcacac ctgccgtttc gtcgacgagc tggcgtacaa ggaatacatc acggatcacc
      241 tgccggcgca cgagcatctg ggcgaactgg tacaggaaaa gctgctgtat taccccaccg
      301 tgacgcgtga agcgttccag aaccgcggcc gcatcaccga actgatcgaa accgaaaagc
      361 tgttcgccga cctcggcgtg ccgggcttct cgctcgaaga cgaccgtgtg atgctgtgcg
      421 gcagcccgca catgctgcgc gacacgcgca agctgctcga cgacctgggt ttccaggaag
      481 gcagcaacaa cgcgccgggt cactacgtgg ttgaaaaggc gttcgtgggt taaacggacg
      541 tccggattga gcggctcgcg ccgcctggtc agcagaaagg agccctcagg ctcctttttt
      601 attgccggtt acaaatcggc accgttgttc taacatttgg cgtcagtatt cttcctacaa
      661 atccgccctt acccaatatc aaccattgca tgaaatatag gcgccaaccc ttgatctgcc
      721 cgcatttatt cttttttttc agatatcatc gcggcgcacg aacagttgtt tgacatctcc
      781 gtcatgaggc gattgagttg ttactgaatg taaattccgt tacgtcatgc tgtagtgatt
      841 tgtccgtcgc gcgcacaacg ggtcaagcac atcactggag gccagagact cgcatgcgtt
      901 cttttgtctt cgcagcgccg atgcgcaacc gtttcgccgt ccacactgct tcctgagagg
      961 gaaagtcatg gatacgtcga ctgtcgtccc gttcagcaac cacaatccgc ccacccctgc
     1021 atacaccgcg ccgcaatccg ctgctgagcg ggagctcgcg caactgcgcg ctcgcgtgcg
     1081 cgaactgtct gccgaactcg ttcaggcgca ggaagctgcc tgccgtcatg tggcgcgcga
     1141 gttgcacgac ggcgtgggcg ccgagttgac cgcgacgcgc ttcgcgcttg ccggcgtcga
     1201 aacctggctg cccgccgatg cgccgccgca gtgctccgcc gcgctcgccg tcgcgaatcg
     1261 ttcgctcgac gccgtgtgcg ccgcgagccg ccaggccgtc gcggaattgc atgcaccgtc
     1321 gctcgaaacg ggcattgtcg gggcgctgtc gcaatggacc ggcgatttcg ccgcgcgcac
     1381 gaacctgcgc acgagtttcg tctgcgccgc cgatgtgcgc ctcacccgtc tgcccgccga
     1441 cgcagcgctg gccgtgttcc gggtcgccca ggaagcgctg aacaatattg ccaggcacgc
     1501 ccgcgccgaa tccgccgacg tgcgcatcga agccggccgc cgtcacctga cgctgattgt
     1561 cagcgacgac ggcgtcggca tgacgcgcaa cgcccgcagc cgtcgcggcc atttcggcct
     1621 gagcggcatg caggcgcgct gcgccgcgtt cgacggcacg ctgcggatca cagcccggcg
     1681 gggcgcctca tcgcgtggcg acagcggcaa agcgccgcgc ggcacgaccg tgcgggcacg
     1741 ctttgcatgg gatgcgatgc tggccctcgc gccgggcgcc aggcgtcggg cgctgcaatc
     1801 gtgcagcacg ccgtcatgag cttgcgcatc ctgctcgtcg acgaccacgc ggtggttcgc
     1861 cagggcgtcc gccagttgct gctcgaccgc ggcgtggcgc gcgaagtgac cgaggcgcaa
     1921 agcggcgccg aagcgctcga cgccgtcgcc caacacagtt acgacgtggt gctgctggac
     1981 atttcactgc ccgacatgaa tggcgtcgaa gtgctcaagc gtctgaagcg caaagcgccg
     2041 cgcgtggccg tgctgatgtt ctcgatgtac cgcgaggacc agtacgccgt acgcgcgctg
     2101 aaggccggcg ccgtcggcta cctgtcgaaa accgtcgacg ccgcgcagat gatcggcgcg
     2161 atccagcagg ttgcggcggg tcgcaaatac gtcagcccgg cgatggccga agcgctggcg
     2221 gactatgtct ccttcgacgg cgaacagttg ccgcacgaaa agctctccga ccgcgaatat
     2281 cagacgctat gcatgcttgc atccggcaag cggctgacgg acatcgccat ggcgctatcg
     2341 ctatcggtga agacggtcag cgtgtaccgc acgcgactgc tcgagaaaat gaagctacgc
     2401 aataacgccg aactgacgtt ctatgtgatg agcaaccgtc tcgtcgatct gaatccggcg
     2461 atggccggct gacgttctct ttgcgtgatt gctcaatagc gcgcgattgc ctgcacccag
     2521 attgcaggag tgcgacttcc ccgatttggg gcgcgcagcg aacgacgcct gatcttgttc
     2581 agtcccgcgg ataactgtct gattcgcggc ggcgcagctc gatgcgggca cgcaagtcga
     2641 gatcgaaaag gctttgcgcc cgtttctcgt gctcaggcag aacgccacgc ccagagggct
     2701 tcagccagac ctgctcgcgt tgcccagcaa cggtgaaaac accgtcccaa aatggtgcag
     2761 aggtaaaatc caaggtttcc cgacattcgg cccgcaagat gcgtgcacta ctggagaccc
     2821 accgccaatg tccctgtttc gcaaaaagag tgtcgagcac atgatcgcga cgagcgctca
     2881 gaacgccggc ctgaagaaag cgctcggcgc gctcgatctg actttcctcg gcgtcggcgc
     2941 cattatcggc accggcattt tcgtgctgac cggcacgggc gccgttcagg ccggcccggc
     3001 gctgatggtt tcgttcctga ttgcagcggt tgcctgcggg ttcgcggcgc tcgcctatgc
     3061 cgagttcgcg tcgaccattc cggtcgccgg atccatctat acgtattcgt acgcgacgct
     3121 cggcgaactg gccgcgtgga tcatcggctg ggacctgatg ctcgaatacg ggctcgcgac
     3181 atcggcggtg tcggtcggat ggtcgggcta tctgcaatcg ctgctgtcgg gcttcggcgt
     3241 gagcttgccg gttgcgctga cagcggcacc gggcgccttg ccggggcacg agaccttgtt
     3301 caacctgcct gctttcctgg tcatgatggc gatcaccgcc ttgttgtcgc tcggcgtgcg
     3361 tgaatccgcg cggatcaaca acatcatggt ggcgatcaag gtgaccgtgg tgctgctggt
     3421 gatcggcgtg ggcgtcttcc acgtgacgcc ggccaactgg catccgttca tgccgaacgg
     3481 ctggaacggc gtgttcggcg cggcggcggt catgttcttc gcgttcatcg gtttcgactc
     3541 ggtgtcgtcg gcggcggaag aggtgaaaaa
//
LOCUS       DATASH010000855        10491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5881244,
            whole genome shotgun sequence.
ACCESSION   DATASH010000855 DATASH010000000
VERSION     DATASH010000855.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 10491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10491
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5881244"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VHV99_22945"
     CDS             <1..292
                     /locus_tag="VHV99_22945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638279.1"
                     /translation="EPLSFAYFSLRQAKKSRCLPRTGATLIDQYQFKERPTPQANRKR
                     APRRQTTQGKANTTGKQKASAAQANNSGKGQHHRQTESEPHAGKQLKERPTP"
     gene            complement(660..1103)
                     /locus_tag="VHV99_22950"
     CDS             complement(660..1103)
                     /locus_tag="VHV99_22950"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEX3638280.1"
                     /translation="MSNPIKSSSAPSAASCTPLPAKRSGPLRALYALRHSCDGIVATL
                     REESAFRQEIALAAVLLPCAALMPVGAIERVLLIVSVLLVLLVELLNSSIEAAIDRIS
                     LERHALSKRAKDCGSAAVTMALLICAVTWGVLCGPLVYHWLRAQM"
     gene            1464..4049
                     /gene="bcsB"
                     /locus_tag="VHV99_22955"
     CDS             1464..4049
                     /gene="bcsB"
                     /locus_tag="VHV99_22955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004547646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HEX3638281.1"
                     /translation="MKPVVSFSLSLTALVGALYAATLAAAPSALPLSSVRVPSSATVA
                     VLPSPAAPGTAANGARAMNATHATAAALATNTTAATAATAATAATAATAATAATNASS
                     ASSASNASNATNATNATNATNATNATNATNATNATNASNAAASAAGSTTVHIPFSALG
                     AYRPLNLRGLEDVSVVNVGVRLDRVVNAARLRLRYTYSPALVFPISHFKVSINDEVVA
                     TVPFDREHAGQVVTQDIPLDPRFLTDYNQLGLRFIAHYTLDHCEDPDNSSLWADVSPT
                     SEIILDTSPIRLPNDLALLPAPFFDRRDVNRLRLPFVLPAAADNATLRSAGVLASWFG
                     ALADYRQARFPVSSTLPSDDQAVVVARSAQLPAALKLPPVNGPMLIVTDNPASVYKKL
                     LIVTGRTAAEVDQAAAALVLGRTAMAGPAVQVAQIDIGAPRKPYDAPRWLPVDRPVAF
                     KELIDDPSQLQVRGGSPDPIRLNLRVPADLFSWNGAGVPLNLKYRYTAPTVQNNSILA
                     VQINDQLVKSYRLPPASADDAQGRLQLPVLSSNGNRSTSALDIPAFRVGSSNQLQLRF
                     NLDSQKTGLCQGVASNPVQAAVDPDSTIDFSNFVHYAQMPNLAYFSNSGFPFTRYADL
                     SQTAVVIPAHPAPEELEALLTMLGHMGQWTGYPALRVQIARPAEITQLPGKDLLVIGG
                     NGSTPWFDRWHQALPLTIGANPNDAGEGVEGGKVTRATFSVKEQWHNGAHLPDGSADL
                     ERDGSLAALMGFELPGSKGRSVVALTATDQHQLLQLLDVLEKPDRVAQLQGDVALVRD
                     GEVDSMRVGEPYLVGYVPWYARLWSKAIQHPILLGVLGAIAGLLLAVGAFTALQELAA
                     RRRGV"
     gene            4053..5534
                     /gene="bcsZ"
                     /locus_tag="VHV99_22960"
     CDS             4053..5534
                     /gene="bcsZ"
                     /locus_tag="VHV99_22960"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HEX3638282.1"
                     /translation="MLPVKWKASLRQAPGAALAARMRLALVADTTVATRVSSGLSIRA
                     VSRASTSVAIGVAATLASGAFAASLSLPANAANNNDNAATTAAAATPATTTATTTTTT
                     TAPATAPAPAPAPAPAPAPAPAPRLTTPTPASCTAPAWPRWQQFKRDFISPEGRVIDV
                     GSADSRTVSEGQSYALFFALVADDRTTFDSILNWTEAHLAQGDLTAHLPAWLWGRSDS
                     GQWGVLDANASSDADLWIAYALLEAGRVWQERSYTARGAVLAKRVLDEETASVPGLGL
                     TLLPGPVGFHPAPDVWRVNPSYSPPQVLRGIGTLLPDDARWTRLLASTGRVLLDTAPH
                     GFSPDWALYRTKQGFAPDAQTQGESAYNAIRVYLWAGMLDPRDPLAQPLLTRFAPFAG
                     FIAAHGAPPEKVDTTNGSVGANMGNAGFSAAAVPFLDARGERAFGDAQIARMEQLDRE
                     TPAGYYTSVLSLFGLGWRDGRYRFAADGTLNLRWSSPCGVVSR"
     gene            5810..9586
                     /locus_tag="VHV99_22965"
     CDS             5810..9586
                     /locus_tag="VHV99_22965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase subunit BcsC-related outer
                     membrane protein"
                     /protein_id="HEX3638283.1"
                     /translation="MARMWSTKHRDDLALQSIQKALLIAPDDPSLLAQQVRTQLRLGQ
                     AQAAQTTLARLRAKAPGTLATQQIEDEFRVATNGREELATIRLMARSGQSAEATRRLV
                     ALFPHGAPSGSLGAEYYQIIAGTPEGRVQAVDALRQRIAADPSDVDSALVLANLLGAR
                     GETRAEANRIAWNLATRPDSDRPASLDAWRHVLQSAGADPAYLAALQAYLQLVPDDTE
                     FKDRVGVIEAQLEAQRQLERNPDYIAQQRGLQALARNDLTTAEPLLARAAQARATDPE
                     AVGGLGLVRMREGRRDEARTLFLRAAVLAPDNRGKWDSLARAAQFWGTLAQGREAAAA
                     GRPQDAERAARAALAMEPANADAKLMLADALLAQHDWSAAEPLLRDLLAAREPNLAAL
                     RSTQTLFENTGRAAQVAPLLDALQSRFTAADDRQSLAQLRADILSAEAEKLLAAGQRG
                     PAAQRYEAALRLAPDAPWTRFALARLYRDLGLPQLGRVVMDDGLAVSASPEMRYASAL
                     YRNSLDDIDGAQAVLAPIAGKDRTDGVRALERNLQAQQRLAQARAAFAQDDLAGATHA
                     LDQARSLAGDDPNLLAAVGALWIDQGQAERGLALLQNWIDTHPQQTDADVRLRYGDLL
                     GSAKRNDALDAWLTSLRSDAHLSPAQSARLEDQALRSILRQTDDALDRQDYAAARQLL
                     GRVSPAGRRDKRYAFEVADLERLQAHYAAARAALAPVLAATPNDPDAQLALARVLEQS
                     GERRTALDLVRTVVDGAAPDDVDTRLSAARRLAALRHPQEAQQITAALRIAYPARPDV
                     TVQSGRVAQDLGQYEDAASLYRLSLSQERAAGVTAGPDGTPAQAALADLQERRDPELE
                     TAWIPASKSGDAGISQFRAWQAPVYVQIPYRYDGHFFFHADTVHIDAGTLDVSDANAS
                     TRDTFGTLGAADLLAQSHNVAPQTVADYAATLPYLHQSATGVALGTGYTSDAWRFDFG
                     TTPLGFPVHYLVGGVRYQFDAGPASFSISGSRRPETSSLLSYAGMHDPLTGAVWGGVR
                     RDGIDFHSALDVGTASVFADVGAGMLTGRNVASNQEVTLRTGFTLPVYQRVNTLVSTG
                     LVGNVWHYTNNQRFYSFGQGGYYSPQRYLSLGVPIEWAGRRDALNWDLTATVGVSNSY
                     EKASPYYPNGLPALNSASAALNDLWKTASSAPGFSNLVNPASSTHGVAFSYGLSGIVQ
                     YRFNHYLVAGAQLSIDRSHDYAPSSALLYMRYTFEARKQDNSLSPTPVRLYSSF"
     gene            9622..>10491
                     /locus_tag="VHV99_22970"
     CDS             9622..>10491
                     /locus_tag="VHV99_22970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BcsE family c-di-GMP-binding protein"
                     /protein_id="HEX3638284.1"
                     /translation="MSTVSNSLKPTPAGAFVRRLLRAVRRAGSASDARLLGQLAIDAL
                     PAEWAALETGKLYAIYAAARTPGADALLWESAREAQARDVTVVLARARAQVAKRLREL
                     GFSSHAPASGWPRNLSVLAMPPAGPGDAGPHGVPPVPLAKVFGGLRALKRFGFRSRSL
                     YFVEGAERWFNWDDPAALAREGRMLANWCAVRDITVVLLLGSQSDVNADVNADADTDI
                     DAAAGSLFIEPTLDSAAAQLGRNEFHAACAGVARMERTHGELLWQVDFWRSDRTLVTG
                     EVRSLRFTDNGRLS"
BASE COUNT         1864 a         3644 c         3346 g         1637 t
ORIGIN      
        1 tgagccgctt tcttttgcct acttttcttt gcggcaggca aagaaaagta ggtgcctgcc
       61 ccgcacaggg gcgacgctaa tagaccaata tcaattcaag gaaaggccaa caccacaggc
      121 aaacagaaag cgagcgccgc gcaggcaaac aacgcaagga aaggccaaca ccacaggcaa
      181 acagaaagcg agtgccgcgc aggcaaacaa ctcagggaaa ggccaacacc acaggcaaac
      241 agaaagcgag ccccacgcag gcaaacaact caaggaaagg ccaacgccgt aggcaaacag
      301 aaaacgagca ccgcccgagc agacaattca ggtaaagcgc catccagcac cttcatctac
      361 gacagcgcgg ttagcgccca aaacccaccc cgccaccgcc gtgactgtcg cgttactgca
      421 acaacacggc atccgcgtct tcccaggaat cgcaaatcgc caagtcgcca gcagctatcg
      481 cattcgaatc agacgagcag ggcgcataga aatacgccaa cgcaccatcg aagagaacca
      541 gaaacccgaa ctttcaccct gcgctaacgt ttgcgcatcc caatccatcc acacgcaaac
      601 gatcaatacg cattacagaa gaaatcacgc gcacagcatg cgcgacacct ggccgcgact
      661 cacatttgcg cgcggagcca gtgataaacg agcggcccgc acaacacgcc ccacgtcacc
      721 gcgcagatca ggagcgccat cgtgaccgcc gcgctgccgc agtccttggc acgcttggac
      781 aatgcgtgcc gctcgagcga aatgcgatcg atcgcggcct cgatgctcga attcaacaac
      841 tccaccagca gtaccagcag cacggaaacg atcagtaata cccgctcgat cgcgccgacc
      901 ggcatcaacg cggcgcaagg cagcaaaacc gccgcgagag caatctcctg acgaaaagcg
      961 ctttcctcgc gcaacgtcgc aacaatgccg tcgcaggagt gccgcagcgc atacagcgca
     1021 cgcaaaggcc cgctgcgttt agcaggcaac ggcgtgcaag aagccgccga aggggccgaa
     1081 gaagacttaa tcggattgct catgatctgc ttcgagtcaa ggtggccgca gcatgcctca
     1141 aatgtcacaa attgtcgcgg acttgtgata tcttctcgcc cgcccaatgg cgccggaccc
     1201 ctgagccaca gtcctcggaa aaagcgcgaa gcaggcatcc ggcacgctat gccggacaca
     1261 ctccatgtgt tggccgtttg tcgtgtcgct cgtcattcaa ggttgtgcgg ggtcgtccgt
     1321 atacaagcgt aggggaccgc atcgcggtca tgtcgcattt ccgtttttca cgttttccgc
     1381 aaacttctgc ggcaggccga attcgcaacg atgtcccacg caatcatggg gcggatggcc
     1441 gacgtccaac aagctgatcg tccatgaaac ctgtcgtttc gtttagtctg tcgctgacgg
     1501 cgctcgttgg cgccttgtat gccgctacgc tagccgccgc gccttccgca cttcctttgt
     1561 cttccgtgcg cgttccgtcg tccgccaccg tcgctgtact gccgtcgccc gctgcgccgg
     1621 gcaccgctgc caacggcgcg cgtgcaatga acgcaaccca cgcaaccgcc gccgcgctcg
     1681 caaccaacac aacagcggca acggctgcaa cggctgcaac ggctgcaacg gccgcaacgg
     1741 ctgcaacggc cgcgaccaac gcgtccagcg cgtccagcgc gtccaacgcg tccaacgcga
     1801 ccaacgcaac caacgcaacc aacgcaacca acgcgaccaa cgcgaccaac gcgaccaacg
     1861 caaccaacgc aacgaacgcg tccaacgccg cggccagcgc agccggctcg accacggtgc
     1921 acataccgtt ctccgcgctg ggcgcgtatc ggccgctgaa tctgcgcggt ctcgaggacg
     1981 tgagcgtcgt caatgtcggc gtgcggctcg atcgtgtggt gaacgcggcg cggctgcgtt
     2041 tgcgctacac gtattcgccg gcgctggtgt tcccgatctc gcacttcaag gtgtcgatca
     2101 acgacgaagt ggtggcgacc gtgccgttcg atcgcgagca tgcagggcag gtcgtcacgc
     2161 aggacatccc gctcgatccg cgtttcctga ccgattacaa ccagcttgga ctgaggttca
     2221 tcgcgcatta cacgttggac cattgcgaag atccggacaa ctcgtcgtta tgggccgacg
     2281 tgagtccgac gagcgaaatc attctcgaca cgtcgccgat ccgcttgcct aacgatctcg
     2341 cgttgctgcc ggcgccgttt ttcgaccggc gcgatgtgaa ccgcttgcgt ctgcccttcg
     2401 tgctgccggc cgccgccgac aacgcgacgc tgcgcagcgc cggcgtgctg gcctcgtggt
     2461 tcggcgcgct cgccgattat cggcaggcgc gttttccggt ctcgtccacc ttgccgtcgg
     2521 acgatcaggc cgtggtggtg gcgcgcagcg cgcagttgcc cgcggcgttg aagctgccgc
     2581 cggtcaatgg gccgatgctg atcgtcacgg acaatccggc atccgtctac aagaagctgc
     2641 tgatcgtgac cggccgcacc gccgcggaag tcgatcaggc ggccgccgcg ctcgtgctcg
     2701 gcaggacggc gatggcgggt ccagcggtgc aggtcgcgca gatcgatatc ggcgcgccgc
     2761 gcaagcccta tgacgcgccg cgctggttgc cggtcgaccg tccggtcgcg ttcaaggagt
     2821 tgatcgacga tccgagccag ttgcaggtgc gcggcggttc gccggaccca atccgcctga
     2881 acctgcgcgt gcccgccgat ctgttttcat ggaacggcgc gggcgtgccg ctgaatctga
     2941 agtaccgcta tacggcgccg acggtgcaga acaactcgat cctcgccgtg cagatcaacg
     3001 atcaactggt caagtcgtac cggctcccgc cggccagcgc cgacgatgca cagggccgcc
     3061 tgcaactgcc ggtgctgtcg tcgaacggca accgttcgac gagcgcgctc gatattccgg
     3121 cgttccgcgt cggcagctcg aaccagttgc agttgcgctt caatctcgat tcgcaaaaga
     3181 ccgggctgtg tcagggcgtc gcgtcgaatc ccgtgcaggc ggcggtcgac ccggactcga
     3241 cgatcgactt ctcgaacttc gttcattacg cgcagatgcc gaatctcgcg tacttctcga
     3301 atagcggctt tccgttcacg cggtatgccg atctgtcgca gacggccgtg gtgattcccg
     3361 cgcatcccgc acccgaagaa ctcgaggcat tgctgacgat gctcggccac atggggcaat
     3421 ggaccggcta tccggcgttg cgcgtgcaga tcgcgcggcc ggccgagatc acgcaactgc
     3481 cgggcaagga tctgctggtg atcggcggca atggttcaac gccttggttc gatcgttggc
     3541 atcaggcgct gccgctgacc atcggcgcga acccgaacga cgctggcgaa ggtgtcgaag
     3601 gcggtaaggt cacgcgcgcc acgttttcgg tgaaggagca gtggcacaac ggcgcgcatc
     3661 tgccggacgg cagcgcggat ctcgaacgcg acggctcgct ggccgcgctg atgggtttcg
     3721 aattgcccgg cagcaaaggg cgcagcgtcg tcgcgctgac ggccaccgat cagcatcagt
     3781 tgctccagtt gctcgacgtg ctggaaaagc cggaccgggt cgcgcaactg caaggcgacg
     3841 tcgccctggt gcgcgacggt gaagtcgaca gcatgcgcgt cggcgaaccg tatctggtcg
     3901 gctatgtgcc ctggtatgcg cggctctgga gcaaggcgat tcagcatccg atcctgctgg
     3961 gtgtgcttgg cgcaatcgcc gggctgctgc tggcggtcgg cgcgttcacc gcgttgcagg
     4021 aactggccgc gcgccgccgg ggagtctgac cgatgctgcc ggtgaagtgg aaagcgtcgt
     4081 tgcggcaggc gccgggcgcg gcgctggcgg cgcgcatgcg cctggcgctg gtggcggaca
     4141 caacggttgc tactcgcgtg tctagcggct tgtctatccg cgcggtaagc cgtgcgtcga
     4201 ccagcgtggc catcggcgtg gccgccacgc tcgcgagcgg cgcgttcgcc gcgagcttgt
     4261 cgctgcccgc caacgccgct aacaacaacg acaacgcagc cacaaccgca gccgcagcca
     4321 cacccgcaac cacaaccgca accacaacca caaccacaac cgcacccgca accgcacccg
     4381 cacccgcacc cgcacccgca cccgcacccg cacccgcacc cgcacccaga ctcaccacac
     4441 ctacaccagc ctcctgcaca gcccccgcat ggccgcgctg gcagcagttc aagcgcgact
     4501 tcatctcgcc cgaagggcgc gtgatcgacg tcggctcggc cgattcgcgc acggtatccg
     4561 aaggtcagtc atacgcactg ttcttcgcgc tcgtcgccga cgaccgcaca acctttgatt
     4621 cgatcctcaa ctggaccgag gcgcacctcg cgcaaggcga cctgaccgcg catctgccgg
     4681 cctggttgtg gggccgctcg gacagcgggc aatggggcgt gctggatgcg aatgcttcgt
     4741 ccgatgccga tctgtggatc gcgtatgcac tgctcgaagc cggccgcgtg tggcaggaac
     4801 gcagctacac ggcgcgtggc gcggtactcg cgaagcgcgt gctcgacgag gaaacagcca
     4861 gcgttcccgg tctcggcttg accctgttgc cagggccggt gggctttcat ccggcgccgg
     4921 acgtgtggcg cgtcaatccg agttattcgc cgccgcaggt gctccgcggc atcggcacac
     4981 tgctgccgga cgacgcccgc tggacgcgtc ttctcgccag caccggccgg gtactgctcg
     5041 acaccgcgcc ccacggcttt tcaccggatt gggccctgta ccgcaccaaa cagggtttcg
     5101 caccggacgc ccaaacccag ggcgagagcg cctataacgc gattcgcgtc tacctgtggg
     5161 ccggcatgct cgatccacgc gatccgctcg cgcaaccgct gctcacccgc tttgcaccgt
     5221 tcgccggctt tatcgccgcg cacggtgcgc cgccggaaaa ggtcgatacg acgaacggca
     5281 gcgtcggcgc gaacatgggt aacgccggtt tctcggcggc agccgtgccg tttctcgacg
     5341 cgcgcggcga gcgcgcgttc ggcgatgcgc agatcgcgcg catggaacaa ctcgatcgcg
     5401 agacgccggc cggctactac accagcgtgc tgagtctgtt cgggctcggc tggcgcgatg
     5461 gccgctatcg ctttgccgcc gacggcaccc tcaatctgcg ctggagttcg ccgtgcggcg
     5521 tcgtttcccg ataacgtgcg cagcgctgct gctcggtctc gcggcgcaaa tggcgccggt
     5581 cgccgcatgg gcaggggcgc aggcgggcac gcagacaatc acgcagccgg gcacgcagac
     5641 aaccacccag ccgggcacgc aaataagcaa gcatccaggc acccagccgg gcactcaaat
     5701 aagcaagcgt ccaagcaccc agccggccgc gcaaataagc acgcaaccag gcaagcaacc
     5761 catcaccacc gcgccatcgc aaaacaccgc cgcgcagcgc ctgttcgcga tggcccgcat
     5821 gtggtcgacc aagcatcgcg acgatctcgc gttgcagtcg atccagaagg ccttgctgat
     5881 cgcgccggac gatccttcgc tgctggccca gcaagtgcgc acccagttgc gtctcggcca
     5941 ggcccaggcc gcgcagacga cgctggcgcg gctgcgggcc aaggcccccg gtacgctggc
     6001 cacgcagcag atcgaagacg agttccgcgt cgccaccaac ggccgcgagg aactggcgac
     6061 gatccgcctg atggcgcgta gcggccagtc cgccgaagcg acgcggcggc tggtcgcgct
     6121 gtttccgcat ggcgcgccgt cggggtcgct cggcgccgag tactaccaga tcatcgcggg
     6181 cacgcccgag ggccgcgtgc aggcggtcga cgccttgcgt cagcgaatcg ccgccgatcc
     6241 gtcggatgtc gattcggcgt tggtgctcgc caatctgctg ggcgcacgcg gcgagacgcg
     6301 cgccgaggcc aaccgcatcg cgtggaatct cgccacgcgg ccggattcgg atcgcccggc
     6361 gtcgctcgac gcctggcgtc acgtgctgca atccgcgggc gcggacccgg cttatctcgc
     6421 ggcgctgcaa gcctatctgc aactcgtacc ggacgacacc gagttcaagg accgggtcgg
     6481 cgtgatcgaa gcgcagctcg aggcgcagcg ccaactcgaa cgcaatcccg actacatcgc
     6541 gcagcaacgc ggcctgcagg cactcgcgcg caacgatctg acgacagccg aaccgctgct
     6601 cgcacgagca gcacaagccc gcgcgaccga tcccgaggcg gtcggcggcc tcggtctggt
     6661 tcgcatgcgc gaagggcggc gtgacgaagc ccgcacgctg tttctgcgcg cggccgtact
     6721 cgcgccggac aatcgcggga aatgggacag cctcgcgcgt gccgcgcagt tctggggcac
     6781 gctggcgcag ggccgcgagg ccgccgcagc cgggcggccg caggatgccg agcgggccgc
     6841 gcgcgccgcg ctcgcgatgg agccggccaa cgccgacgcc aaactgatgc tcgccgacgc
     6901 gctgctcgcc cagcacgact ggagtgccgc ggaaccgctg ctgcgcgacc tgctggcggc
     6961 gcgcgaaccc aacctggcgg cgctgcgcag tacccagacg ctgttcgaga acaccggccg
     7021 cgcggcccag gtcgcgccgc tcctcgacgc attgcaaagc cgcttcacgg ccgccgatga
     7081 ccgccagagc ctcgcgcaac tgcgcgccga catcctcagc gcagaagcgg aaaagttgct
     7141 cgcggcgggc cagcgcggcc cggccgcgca gcgctacgag gcggcgttgc ggctcgcgcc
     7201 cgatgccccg tggacgcgct ttgcgctcgc gcgtctatat cgcgatctgg gtctgccgca
     7261 acttgggcgg gtggtgatgg acgatggcct cgccgtgtcg gcatcgcccg aaatgcgtta
     7321 tgcgagcgcg ctctatcgca actcgctcga cgacatcgac ggtgcgcaag ccgtgctcgc
     7381 tccgatcgcc ggcaaggacc gcacggacgg cgtgcgcgcg ctcgagcgca atctgcaagc
     7441 gcagcagcgg ctcgcccagg cgcgcgccgc attcgcgcag gacgaccttg cgggcgcaac
     7501 acacgcgctc gaccaggcac gctcgctggc cggcgacgat ccgaacctgc tggccgcggt
     7561 cggcgcgctg tggatcgatc agggccaagc cgagcgcggc ctcgcgttgc tgcagaactg
     7621 gatcgacacg catccgcagc agaccgacgc ggacgtgcgt ctgcgctacg gcgatctgct
     7681 cggcagcgcg aaacgcaatg acgcattgga cgcgtggctg acatcgctgc gtagcgatgc
     7741 acacctgagc cccgcgcaaa gcgcgcgtct cgaagaccag gcgttgcgct cgattctgcg
     7801 ccagaccgac gacgcgctcg atcgccagga ctacgccgcg gcgcggcagt tgctcggccg
     7861 cgtcagtccg gccggccggc gcgacaaacg gtatgcgttc gaagtcgccg atctcgaacg
     7921 tctgcaggcg cactatgcgg cggcgcgcgc cgcgctggcg ccggtgctgg ccgcgacgcc
     7981 gaacgatccg gacgcgcaac tggcgctcgc ccgggtgctg gagcaaagcg gcgaacgccg
     8041 cacggcgctc gatctcgtgc gtacggtggt cgacggcgcg gcgcccgacg acgtcgatac
     8101 gcgcctgtcc gcggctcgcc gtctggccgc attgcgccac ccgcaggaag cgcagcagat
     8161 caccgcggcc ttgcggatcg cgtatcccgc gcggccggac gtgacggtgc aaagcggacg
     8221 ggtcgctcag gatctgggcc agtacgagga cgccgcatcg ctgtaccgcc tgtcgcttag
     8281 ccaggaacgc gcggcgggtg tgacggccgg ccccgacggc acacctgccc aggccgcgct
     8341 ggccgatctg caggagcgcc gcgatccgga gctcgaaacc gcatggatcc ccgcgtctaa
     8401 atcgggcgac gccggcattt cgcagtttcg cgcgtggcaa gcgcccgtgt acgtgcagat
     8461 tccgtatcgc tacgacggcc actttttctt ccacgccgat acggtgcata tcgatgccgg
     8521 cacgctcgac gtcagcgacg cgaatgcgtc gacgcgcgac acgttcggca cgctgggcgc
     8581 cgccgacttg ctcgcgcagt cgcacaacgt ggcgccgcag accgtggccg actacgccgc
     8641 gacgctgccg tatctgcatc aatcggccac cggcgtggcg ctcggcacgg gctatacgtc
     8701 cgatgcgtgg cgcttcgact tcggcacgac gccgctcggt tttcccgtgc attacctggt
     8761 gggcggcgtg cgctatcagt tcgacgcggg accggcgagc ttttcgatca gcggctcgcg
     8821 ccgtccggag acgagcagtc tgctgtcata cgcgggcatg cacgatccgc tgaccggcgc
     8881 cgtgtggggc ggcgtgcgcc gcgacggcat cgacttccac tcggcgctcg atgtcggcac
     8941 ggccagcgtg ttcgccgatg tcggcgcggg catgctgacc ggccgcaatg tggcgagtaa
     9001 ccaggaagtc accctgcgca ccggcttcac gctgccggtc taccaacgcg tcaacacgct
     9061 ggtgagcacg gggctggtcg gcaacgtctg gcactacacc aacaatcaac gcttctattc
     9121 gttcgggcag ggcggctatt acagcccgca acgctatctg tcgctcggcg tgccgatcga
     9181 atgggcgggt cggcgcgatg cgctgaactg ggacctgacg gccaccgtcg gcgtgtcgaa
     9241 ttcgtatgag aaggcctcgc cgtattaccc gaacggcttg cccgcgctga acagtgcatc
     9301 ggccgcactc aacgatctgt ggaagacggc cagctcggcg ccgggcttca gcaacctcgt
     9361 caatccggcc agctcgacgc atggcgtcgc gttttcgtat ggcttgagcg gcatcgtgca
     9421 gtaccgcttc aaccattatc tggtggcggg cgcgcagctg agcattgacc gttcgcatga
     9481 ctacgcaccg agttccgcgc tgctgtacat gcgctatacc ttcgaggcac gcaagcagga
     9541 caacagtctg tcgccgacgc ccgtgcgcct ttactccagc ttttgagcgg cgctacaacg
     9601 aacgagatca ggaagccacg cgtgagcacc gtttcgaatt cactcaagcc gacgccggct
     9661 ggcgcctttg tgcgacgcct gctgcgggct gtccgccggg cgggatcggc tagcgacgcg
     9721 cggctgctcg gccaactggc gatcgatgcg ctgcccgccg aatgggccgc gctcgaaacc
     9781 ggcaagctct acgcgatcta tgccgccgcg cgcacgccag gcgccgacgc gctgctctgg
     9841 gaaagcgcgc gcgaggcgca ggcgcgcgac gttaccgtgg tgctggcacg cgctcgcgcg
     9901 caggtcgcca aacgtctgcg cgaactcggt ttcagcagcc acgcgccggc ctccggctgg
     9961 ccgcgtaacc tgagcgtcct cgccatgccg ccggccggtc ctggcgacgc aggcccccac
    10021 ggggtgccgc cggtcccgct tgccaaggtg tttggcggtt tgcgcgcgct caagcgcttc
    10081 ggcttccgtt cgcgctcgct gtatttcgtc gaaggcgccg agcgctggtt caactgggac
    10141 gatccggcgg cactcgcgcg cgaaggccgg atgctcgcga actggtgcgc ggtgcgcgac
    10201 atcacggtgg tgttgctgct gggttcgcaa tccgacgtca atgcggacgt caatgcggat
    10261 gccgacacgg acattgacgc cgctgccggt tcactgttca tcgagcccac cctcgacagc
    10321 gcagcggcgc aacttggccg caacgaattt cacgcagcct gcgcgggcgt cgcgcggatg
    10381 gagcgcaccc acggcgagtt gctgtggcag gtggatttct ggcgctcgga ccgcacgctc
    10441 gtcaccggcg aggtgcgctc gctgcgcttt accgataacg gccgcctgag c
//
LOCUS       DATASH010000856         4124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2322599,
            whole genome shotgun sequence.
ACCESSION   DATASH010000856 DATASH010000000
VERSION     DATASH010000856.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2322599"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..1066)
                     /locus_tag="VHV99_22975"
     CDS             complement(11..1066)
                     /locus_tag="VHV99_22975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3638285.1"
                     /translation="MSELRVTSLTKSFDGQPVLHGVDLSVERGALLALLGPSGSGKTT
                     LLRVLCGFERADGGTVEIDGVRVAGPGIHVPSEARRIGYVPQEGALFPHLSVADNIVF
                     GLPRQQRRARYRVDELLALVGLPAAYGDRAPQALSGGQQQRVALARALAPSPSLVMLD
                     EPFSSLDAALRIETREAVANALAATGATAVLVTHDQAEALSLGHEVAVLWRGRLVQTD
                     SPEAVYRRPVSRELASFVGDAVIVPGSVDSRNSKQVICEFGTLPIARAMAAGDVDVMI
                     RPEQIRIVADHHMNGDGVQAVVEKVVFYGHDASVMLRSLTGERGVQIRVAGHSHPRVG
                     ERIALSVEGDVVAYPKG"
     gene            complement(1073..2716)
                     /locus_tag="VHV99_22980"
     CDS             complement(1073..2716)
                     /locus_tag="VHV99_22980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX3638286.1"
                     /translation="MSDVVKAVGVSPLPEPRLRKRAPRGLLVAAGLCALLVLMPLAFT
                     VYRAMTFGWDDAIELIFRPLVGELLINTLSITVSATLVSAVVGTAAAWFIERTRLPGR
                     RLWAALTVAPLAMPAFITSYAWVSLSLDLQDFAGALLVISTAYFPLVYLPVAAALRGM
                     DPALEESARSLGCGRVGVFMRVVLPQLRPALLGGMLLVALGVLSEFGAFTLLRFRTFT
                     TQIYAEYRTSFDSSGASVFACILIAMCLFCLALEMRVRGRSRYERVDRGVRRAALRYE
                     LGAWRWAVVAAFVVLAIVTLGVPVGMIGFWLTQSGAAAITPAEVSPELLIESTLSSIG
                     YGCAAAVVTTLACVPLAFLLVRFPSRMATTFERTVFLAQGLPSLVVALAIVSLAVHAL
                     RPLYQSAELLVVAYAILFLPLALVSVRAAFTQAQPRLEETARSLGLSWRETVRRVLLP
                     LAGPGLGAGATMVFISVVTELNSTLLLSPSGTRTLATQVWIDTSTLAFAAAAPYAALL
                     VGISLAASIALFTLLGRSAVAGRAAVLKEGLQAGSPPDK"
     gene            complement(2729..3754)
                     /locus_tag="VHV99_22985"
     CDS             complement(2729..3754)
                     /locus_tag="VHV99_22985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011351132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HEX3638287.1"
                     /translation="MNTTRSLPVLRTLCGIAVLAAAAALPLNSEAATLTLYNAQHEQV
                     VAALAKDFEKQSGVSVKVRNGEGPAMAAQLVAEGSASPADVYFTENSPELMLLDEKGL
                     LAKTDASTLSTIPSRYNSPQGDWVGVLARENVLVYNTKKIDAAQLPASLMDLAKPEWK
                     GRVGIAPSDGDFLPVVSAVIAMKGEAQALDWLKGLKKNSQIFDDDEGVVAAVNRGGVA
                     TGVVNNYYWARLHVQLGDSATRSAIYHFGNGDVGALVNVSGAAVLKSSKNQKEAQQFL
                     AYLVSERAQDLMAKGRIAFEYPLHAGVSPDPLLKPFDQLSPPSLTLEQLGDDSKAAKL
                     MRQAGLL"
     gene            complement(3832..>4124)
                     /locus_tag="VHV99_22990"
     CDS             complement(3832..>4124)
                     /locus_tag="VHV99_22990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HEX3638288.1"
                     /translation="ATNNGAQILRRSYSYNDGTDFYIERWPPWRQETEYDAGLIFVAH
                     QSDPRTGFIPINDRLAKFDMMNQFTTHVGSAVFACPPGVRPGSFIGAGLFET"
BASE COUNT          857 a         1389 c         1220 g          658 t
ORIGIN      
        1 cagcccatgg ctaacccttg ggataggcca cgacgtcgcc ttcgaccgac agcgcgatac
       61 gctcgccgac acgcggatgc gaatgccccg ccacgcgaat ctgcacgccg cgctcgccag
      121 tgagggagcg cagcatgacg ctggcatcgt gaccatagaa cacaactttc tcgaccaccg
      181 cctgaacgcc atcgccgttc atatgatgat cggcaacgat tcggatctgc tccggccgga
      241 tcatcacatc gacatcgccg gcggccatcg cgcgtgcgat cggcaaggtg ccaaactcgc
      301 aaatcacctg cttcgaattc cgggaatcga ccgagcccgg cacgatcacc gcgtcgccga
      361 caaaactcgc cagctcacgc gacaccggcc gccgatacac cgcctccggc gaatcggtct
      421 gcacgagacg gccacgccac aacaccgcaa cctcgtgccc cagcgacaac gcttccgcct
      481 gatcgtgcgt aaccagcacc gcggtcgcgc ccgtggcggc aagtgcgttc gccacggctt
      541 cacgggtttc gatccgcaac gcggcatcga gcgaagaaaa cggttcgtcg agcattacga
      601 gcgagggcga cggcgcaagt gcccgcgcca gtgcgacgcg ttgctgctgt ccgccggaca
      661 gtgcctgcgg cgcacggtcg ccatacgcgg ccggcaggcc aacgagcgcc agcaattcat
      721 ccacacgata ccgcgcccga cgctgctgcc gcggcaagcc aaagacgatg ttatcggcga
      781 ccgaaagatg cgggaacagc gcgccttcct gcggcacata gccaatccgg cgcgcctcag
      841 acggcacgtg aatgcccggc ccggctacgc gcacgccatc gatttcaaca gtgccgccgt
      901 ctgcccgctc gaagccgcaa agcacgcgca acagcgtggt cttgccgctg ccggacggcc
      961 cgagcagagc gagcaaggcg cctcgttcca ccgataaatc gacgccgtga agcacgggtt
     1021 gaccatcgaa ggatttggtc aggctggtaa cgcgaagttc gctcatatcg atttacttgt
     1081 caggcggcga accggcctgt agtccttctt ttaaaacagc cgcacgcccc gcgaccgccg
     1141 atcgcccgag caacgtgaac agcgcaatcg atgccgccag cgaaatgccc acgagcaatg
     1201 cggcgtacgg cgcagcggcg gcaaacgcga gcgtggaggt atcaatccag acttgagtgg
     1261 caagcgtgcg tgtgccgctg ggcgacagca ataacgtgga attgagttcg gtgacgaccg
     1321 agatgaagac cattgtcgcg cctgcgccga ggcctggacc cgccagcggc aagagcaccc
     1381 gccgcacggt ttcgcgccag cttagcccaa gcgatcgcgc ggtctcttcc agacgcggct
     1441 gcgcttgcgt gaacgccgcg cgcacactga ccagcgcgag cggcaggaac agaatggcgt
     1501 aggccacgac cagcagctca gcgctttgat acagcggcct caacgcgtgc acggccagcg
     1561 acacgatcgc cagcgcaacc acgagactcg gcaaaccttg cgcgaggaac acggtgcgtt
     1621 cgaacgtcgt cgccatcctg ctgggaaagc gaaccagcaa gaacgcgagg ggcacgcacg
     1681 cgagcgtagt gacgaccgcg gcggcgcaac cataaccaat cgacgacaac gtcgattcga
     1741 tcagcagttc cggcgacact tccgcgggcg taatggccgc tgccccgctt tgcgtcaacc
     1801 agaaaccgat catgccgaca ggcacgccca acgtcacgat cgccagcacg acgaacgcgg
     1861 cgaccacggc ccatcgccac gcgccaagtt cataacgcaa tgcggcacgc cgcacgccgc
     1921 gatcgacgcg ctcatagcgc gaacggcccc gcacgcgcat ttccagcgcg aggcagaaca
     1981 ggcacattgc gataagaatg cacgcaaata ccgacgcgcc gctgctgtcg aagctcgtcc
     2041 tgtattcggc atagatctgg gtagtgaagg tccgaaaacg caacagcgtg aacgcaccga
     2101 attccgacag aacgcccagc gccaccagca gcattccgcc aagcagcgcg ggccgcaact
     2161 gcggcaacac cacgcgcatg aacacgccaa cgcgcccaca accgagcgag cgcgcgcttt
     2221 cttcgagcgc cgggtccatg ccgcgcaagg cggcggcaac cggcaaatag acaaggggaa
     2281 aatacgcggt ggaaatcacg agcaacgcgc ccgcgaagtc ctgcaaatcg aggctcagag
     2341 agacccacgc ataactcgtg atgaacgcgg gcatcgcaag cggcgcaacg gtgagagccg
     2401 cccacagtcg acgcccgggc aaacgcgttc gttcaataaa ccatgctgcc gccgtcccca
     2461 ccaccgccga cacgagcgtg gcgctcacgg taatggacag tgtattgatg agcaactcac
     2521 ccaccagcgg cctgaaaatc agctcgatag cgtcgtccca gccgaacgtc atcgcgcgat
     2581 acaccgtgaa ggcgagcggc atcagcacca gcagcgcgca caatcccgcc gccacgagca
     2641 aaccccgcgg cgcgcgtttg cggaggcggg gttcaggcag cggggatacg ccaaccgcct
     2701 tcacgacatc gctcatactt tcctgcgctt acagtaatcc ggcctggcgc atcagcttgg
     2761 cggctttgct gtcatcgccg agttgttcaa gcgtcaacga cggcgggctc aactggtcga
     2821 aaggcttgag cagcggatcg ggagaaacac ctgcatgcag cggatattca aacgcgattc
     2881 ggcccttggc catcaggtcc tgcgcacgtt cgctgacgag atacgccagg aattgctgcg
     2941 cttccttctg gttcttgctc gacttcaaca cggccgcgcc cgagacgttc acgagtgcgc
     3001 ccacatcgcc gttaccgaag tgatagatcg cgctacgcgt cgcgctgtcg ccgagttgca
     3061 catgcaggcg cgcccagtag tagttgttca ctacgcccgt tgccacgccg ccgcgattca
     3121 ctgctgccac cacaccttcg tcatcatcga agatctgcga gtttttcttc agacccttga
     3181 gccagtccag cgcttgcgct tcgcccttca tcgcgataac cgcgctcacc accggcagga
     3241 aatcgccgtc gctcggtgca atgccgacgc ggcctttcca ctccggcttg gccaggtcca
     3301 tcagcgatgc cggcagttgc gccgcatcga tcttcttcgt gttgtagacc agcacgttct
     3361 cacgcgcgag cacgccgacc cagtcgccct gtggcgagtt gtagcgcgac gggatggtcg
     3421 agagcgtgga cgcatcggtt ttcgcgagca ggcctttctc gtccagcagc atcagttcgg
     3481 gagagttctc ggtgaagtag acatcggcgg gagaagccga gccttccgcg acgagttgcg
     3541 ccgccatggc cgggccttcg ccgttgcgga ccttcaccga aacgccgctt tgcttctcga
     3601 agtccttcgc cagcgcggcc acgacctgct cgtgttgcgc gttgtacaag gtcagcgtcg
     3661 ccgcttccga gttcagcggc aatgccgctg cggcagccag caccgcgatg ccgcaaagcg
     3721 tgcgcagcac gggcagggag cgtgtggtgt tcattgcaat cattcctttc gttgtggtgt
     3781 gccggcgtgt gccagcgagt gctttagctt gagcttccgg cttagtttga atcaggtttc
     3841 gaacagcccc gcgccaataa acgaaccggg ccgcacgccc ggcgggcatg cgaacaccgc
     3901 gctgcctaca tgcgtggtga actggttcat catgtcgaac ttcgccagac ggtcgttgat
     3961 cggaatgaaa ccggtgcgcg gatcgctttg atgggcgacg aagatcaacc ccgcgtcgta
     4021 ctcggtttcc tggcgccatg gcggccagcg ctcgatataa aaatcggtgc cgtcgttgta
     4081 ggaatacgaa cggcgcagga tctgcgcacc attgttcgtc gcct
//
LOCUS       DATASH010000857         1995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1729313,
            whole genome shotgun sequence.
ACCESSION   DATASH010000857 DATASH010000000
VERSION     DATASH010000857.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1729313"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..679)
                     /locus_tag="VHV99_22995"
     CDS             complement(41..679)
                     /locus_tag="VHV99_22995"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX3638289.1"
                     /translation="MKSFEFRRPLVVGIGGTTRAASSTERALRLALKGAEAEGARTRL
                     FGGTFLHSLPHYAPEHGERSAEQLELIDAVREADAVIIATPGYHGGVSGLVKNALDTL
                     EELRDDKRPYLDGRAVGCVVTAYGWQGGGAVLTSLRSIVHALRGWPTPYGAAVNSLET
                     HFETIDSCSDPKVAEQLATVGRQAAQFALAFNARDALGDRDLRTEGSATIAR"
     gene            1052..>1995
                     /locus_tag="VHV99_23000"
     CDS             1052..>1995
                     /locus_tag="VHV99_23000"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEX3638290.1"
                     /translation="MIEIRNLSQRFAGPRGWIEALHNVNLTIPPGEVFGIIGRSGAGK
                     STLVRTINLLTRPSEGSVIVDGRDLTTLPAAQLRTARRDIGMIFQHFNLLSSRTVFDN
                     VALPLELVGMKRGEIEATVLPLLELVGLTTQKDRYPAQISGGQKQRVGIARALASKPK
                     VLLSDEATSALDPETTRSILDLLRKINKELGLTIVLITHQMDVIKQVCDRVAVLDAGR
                     VVEEGNVVDVFMQPHHEVTRALIGDVIAHELPPALKLRVAERLKTGSGHLLRLAFTGS
                     GVDQPILSETIRRLELDFNILHGQIDEIQGQAFGSLAVL"
BASE COUNT          396 a          649 c          590 g          360 t
ORIGIN      
        1 cgcgccagca gacctccccg tccaccggcg cgagcgaaac tcaccgcgca atagtggcgg
       61 acccctcggt acgcaaatcc cgatcaccca acgcatcccg cgcattaaac gccagcgcga
      121 actgtgcggc ctgccgccca actgtcgcca actgctccgc gactttcggg tccgagcagg
      181 aatcaatcgt ctcaaagtgc gtttccagcg agttgaccgc tgcgccatac ggcgtgggcc
      241 agccgcgcaa cgcatgcacg atcgaacgca aggacgtgag cacggcacca ccgccttgcc
      301 agccataagc tgtcaccacg cagcccaccg cccggccgtc gaggtagggg cgcttgtcgt
      361 cgcggagctc ctccagcgtg tccagcgcgt tcttgaccag gcccgagacg ccgccgtggt
      421 aacccggcgt cgcaatgatc actgcatcgg cttcgcgcac cgcgtcgatc agttcgagct
      481 gttctgccga gcgttcgccg tgttcgggcg cgtagtgagg gagcgagtgc aggaatgtgc
      541 cgccgaacag gcgcgtgcgt gcgccttcgg cttcggcgcc tttcagggcg aggcgaagcg
      601 cgcgttcagt cgatgaggcc gcgcgggtcg tgccgccaat gccgaccacg agcggccggc
      661 gaaattcaaa gctcttcaac gaaatactcc caggagtgca gcgaagccgc gcttggcacc
      721 cgttcgtaca caagaacgcc gcggcatatt ggagacgcga tcacgcgagt tccacgcatg
      781 gatcatgccc ccatcttagc tatcgatatt gcgcgatgga accgagcttt gcttgaatcg
      841 atatgagcat tgcgtatttc gaagccgata aacacccctg ccggcccgcc acgcaacgta
      901 catacggata agtaattagc cttgcgcaat agattattaa gccgtggcgc gggttacgtt
      961 aaactttcgc ctctcgcgaa ccagggatga acggcggtcg gctgcccgag cgaactcgct
     1021 caacgggaag tccgccgctt tttttgttta catgatcgaa atacgcaatc tgtcgcaacg
     1081 cttcgcgggc ccacgaggct ggatcgaagc gctgcataac gttaatttga cgattccgcc
     1141 cggtgaagta ttcggcatca tcggccgcag cggcgccggc aaaagcacgc tggtacgcac
     1201 catcaacctg ctgacgcggc caagcgaagg cagcgtgatc gtggacggcc gcgacctcac
     1261 cacgctgccc gccgcgcaat tgcgcacggc acgccgtgac atcggcatga tcttccagca
     1321 cttcaacctg ctgtcgtcgc gcacggtgtt cgacaacgtc gcgctgccgc tcgagcttgt
     1381 cggcatgaag cgcggcgaga tcgaggcgac cgtgttgccg ctgctggaac tcgtcgggtt
     1441 gaccacgcaa aaagatcgtt atcctgccca gatcagcggc ggccagaagc agcgcgtggg
     1501 gatcgcccgt gcgcttgcca gcaagcccaa agtgctgctc tccgacgaag ccacgtccgc
     1561 gctcgatcct gaaaccacac gctccatcct tgatctgctg cgcaagatca acaaggaact
     1621 cggcctgacg atcgtgctga tcacgcatca gatggacgtg atcaagcagg tctgcgatcg
     1681 cgtcgcggtg ctcgatgccg gccgcgtggt agaggaaggc aatgtcgtcg atgtcttcat
     1741 gcagccgcat cacgaagtca cgcgcgcgtt gattggcgac gtgatcgcgc acgagttgcc
     1801 gcccgcgttg aagctgcgcg tggccgagcg tttgaaaacc ggcagcggtc atttgctgcg
     1861 gctcgcgttc accggctccg gcgtcgacca gccaattctc tctgaaacaa tccgccgctt
     1921 agaactcgat ttcaacatcc tccacggcca gatcgacgag atccagggtc aggcattcgg
     1981 ctcgctggcg gtgct
//
LOCUS       DATASH010000858         2357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4100146,
            whole genome shotgun sequence.
ACCESSION   DATASH010000858 DATASH010000000
VERSION     DATASH010000858.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4100146"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="VHV99_23005"
     CDS             <1..652
                     /locus_tag="VHV99_23005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016347476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3638291.1"
                     /translation="DMIDKAPTDWSDLLKSDYKNAVSLAGDPRSANQAIEAVYAAGLS
                     QSKGDAGKADQAGLKFFADLNRNGNFVPVIGKAASLAQGTTPIIVRWDYNALADRDTL
                     KGNPKVQVMIPKTGVVAGVYVQAISAYAPHPNAAKLWMEYLYSDEGQIGWLTGYCHPM
                     RYNELVAAKKVPQALLDKLPPPSSYKNVVFPTLPKQDASKDTITKHWNDAVGANVK"
     gene            790..1728
                     /locus_tag="VHV99_23010"
     CDS             790..1728
                     /locus_tag="VHV99_23010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX3638292.1"
                     /translation="MSASSDAGSVQPELLVPPALTPTPRVDGPGRASQWLAWIGVMPF
                     FTFALMFLILPTGFLMIGAFQDSQGHFTLANFRELFQPTVMDAYWISFKVSAASALGG
                     ALIGSLLAWAAVQGKLPGWIRPTLMTFSGVASNFAGVPLAFAFICTLGRVGLVTVLLK
                     KYLGFNLYATGFSILSFSGLTLTYLYFQVPLMVLIVTPALDGLRREWREACDCLGGTP
                     YQYWRYVALPVLWPSVLGATLLLFANAFGAVATAYALTGSSLNIVTILLFAQIRGDVM
                     HNQNLGYALALGMVLVTGLSNGGYIWLRSHAERGRR"
     gene            1725..>2357
                     /locus_tag="VHV99_23015"
     CDS             1725..>2357
                     /locus_tag="VHV99_23015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX3638293.1"
                     /translation="MKSQSRVGAWTAVIVGSLYFLIPLIATFEFSLRMKRGTYSFEAY
                     RIVLADPHFQASFGYSILMAILTIVVGLLLVVPTAYWVQLRLPKLRPVVEFITLLPLV
                     VPAIVIVFGYLRIYNSSSIVPFTGNERATDLLLVFGYVTLSLPYMYRAVDAGLRAVDV
                     RSLTEAAECLGANWPTILFKVIFPNIRSGILSGAFLTFAVVIGEFTLASLL"
BASE COUNT          391 a          718 c          759 g          489 t
ORIGIN      
        1 cgacatgatc gacaaggcgc cgactgactg gagcgatctg ctcaagtccg actacaagaa
       61 cgccgtctcg ctggcgggcg acccgcgctc ggccaatcag gcgatcgagg ctgtgtatgc
      121 agccggcttg tcgcaaagca agggcgacgc cggcaaggcg gaccaggccg gcctcaaatt
      181 cttcgccgac ctgaacagga acggcaattt cgtgccggtg atcggtaagg ccgcgtcgct
      241 cgcgcaaggc actacgccga tcatcgtacg ctgggactac aacgcgctcg ccgaccgcga
      301 cacgctgaag ggcaatccga aggtccaggt gatgattccg aaaacaggcg tggtcgccgg
      361 cgtgtacgtt caggcaatca gcgcgtacgc gccgcatccg aacgcggcca agctgtggat
      421 ggagtatctg tactcggacg aaggccagat cggctggctg acgggctact gccacccgat
      481 gcgctacaac gagctggtgg ccgcgaagaa agtgccgcaa gcgctgctcg acaagctgcc
      541 gccgccgtcg tcgtataaga acgtggtgtt cccgacgctg cccaagcagg acgcctccaa
      601 ggacaccatc acgaagcact ggaacgacgc agtcggcgcg aacgtcaagt gagcggcatg
      661 gctaaagtcg tcgcggctct caagcgtatg cgccgcgatg tcgaaccaaa gcgaaaagtt
      721 gtgccgtcgc gcgccgctca ttccgggtgg cgcgcgttcg cgttagccat aaagcccaaa
      781 gggtgagcta tgagcgcttc atcggatgcc gggtctgtgc agccggaatt gctggttcca
      841 ccagcgctaa cgccaacgcc tcgcgtcgac ggtccgggcc gcgcgtcgca gtggctcgcg
      901 tggatcggcg tgatgccgtt cttcacgttt gcgctgatgt tcctgattct gccgaccggc
      961 tttctgatga tcggcgcgtt ccaggactcg caaggccact tcacgctcgc caatttccgc
     1021 gagctgttcc agccgaccgt gatggacgct tactggatca gtttcaaggt gagcgccgcg
     1081 tcggcgctcg ggggcgcgct gatcggttcg ctgctcgcgt gggcggcggt gcagggcaaa
     1141 ctgccgggct ggattcgtcc gaccttgatg acgttctccg gcgtcgcgtc gaactttgcc
     1201 ggcgtgccac tcgcgttcgc gttcatctgc acgctggggc gggtcgggct cgtcacggta
     1261 ctgctgaaga agtatctcgg cttcaatctg tacgcgacgg gattcagcat tctgagtttc
     1321 tccggcctga cgctgaccta cctgtacttc caggttccgt tgatggtgct gatcgtcacg
     1381 cccgcgctcg acggcctgag gcgcgaatgg cgcgaggcct gcgattgcct cggcggtact
     1441 ccgtatcaat actggcgcta cgtcgcgctg ccagtgttgt ggccgagcgt gctcggcgcg
     1501 acgctgctgc tgttcgccaa tgcgttcggc gcggtcgcca cggcttacgc gctgaccggc
     1561 agctcgctga atatcgtcac gatcctgctg ttcgcacaga ttcgcggcga cgtgatgcac
     1621 aaccagaacc tcggctacgc gctcgcgcta gggatggtgc tcgtgactgg tctgtcgaat
     1681 ggcggctaca tctggctgcg ttcgcacgcc gaaagggggc gccgatgaaa agccagtcgc
     1741 gggtgggagc atggaccgcg gtgatcgtcg gttcgctgta ctttctgatt ccgttgatcg
     1801 cgacgttcga gttcagcttg cgaatgaagc gcggcacgta cagcttcgag gcgtatcgca
     1861 tcgtgctggc cgatccgcat tttcaggctt cgttcggcta ctcgattctg atggcaatcc
     1921 tgacgatcgt cgtcggcctg ttgctggtgg tgccgaccgc ttactgggtc cagctgcgtt
     1981 tgccgaagct gcggccggtc gtcgaattca tcacgctgtt gccgctggtg gtgccggcga
     2041 tcgtgattgt gttcggctat ttgcgcatct ataacagcag ttcgattgtg ccgttcaccg
     2101 gcaacgagcg cgcgaccgat ctgctgctgg tgttcggcta cgtgacgctg tcgctgccat
     2161 atatgtaccg cgcggtcgac gccggcctgc gcgcagtcga cgtgcgctcg ctcaccgaag
     2221 cggcggagtg cctcggcgcg aactggccaa ccattctgtt caaggtgatt ttcccgaata
     2281 tccgctcggg cattctgtca ggcgcgtttc tcacctttgc cgtggtgatc ggcgagttca
     2341 cgttggcgag cctgctc
//
LOCUS       DATASH010000859         2710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3313554,
            whole genome shotgun sequence.
ACCESSION   DATASH010000859 DATASH010000000
VERSION     DATASH010000859.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3313554"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2158)
                     /locus_tag="VHV99_23020"
     CDS             complement(<1..2158)
                     /locus_tag="VHV99_23020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016355439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HEX3638294.1"
                     /translation="MASLNKTSLTSAGRSLLKGANSVVQARRTRRIVIGLLLAVIVIG
                     RLGFFAAPPLIRHIAEQQLSAQLDRPASIERIALNPYTLSVEADRVHIGEAPANLGAD
                     AKSGDFVDIERLIVRPSWSSLFRLAPIINELKIDSPRFHIVRFDAQRFNFSDLVEKFS
                     KPSANPSTKPARFSVSNIRVENGRIDFDDRLLKTHHIIDRWSIGIPFIANLASTAELF
                     VTPSLQARIDGSPLSVHGRTKPFSESRESEITLQLDGLDVPQLASYAPASLPVAIKSG
                     RVSTNLDVSFELSADTPVVRVKGTVDLADLAVTDKQDAPLFAAQKLHVNAANLEPLRN
                     VYRLDEVRLAQPDVKLSRDKTGAFNFQKLSATTPASTAVATPASAAKPTDTDTTAAQP
                     LDLAIKHLAIDQGHIVFDDHLMTQSATLGLTALNVTLDDFSTLGKPPARYTLKTAFDH
                     GGGLDASGSFTLPGKNADAKLALSDLPLAPLQPYVANAVAARITDGSLGANLPLQVDW
                     SKPEPSIQVGAGDVTLKSLKLVPQGNNAANIAPVALASAQAKIRKIDVAARTAALDSV
                     VLNGLSIDAKRLKDGSIDLAALAGPHETAPEASATHKVAKANEAGPAWRYQIAQVSLN
                     DSSADFTDESTPRPVKLHIAPLQLGVKQVTDDLKKPLAIDGKLTMNGKGALAVAGTLT
                     AKPLSLALHVDASELDASAFEPYFGSNLNATVSSALL"
BASE COUNT          503 a          765 c          865 g          577 t
ORIGIN      
        1 tcagcagcgc gctcgagact gtcgcgttca ggttactgcc gaagtacggc tcgaacgccg
       61 atgcgtcgag ttcgctggcg tcaacgtgca gcgcgaggct caacggtttc gcagtcagcg
      121 tgccggctac ggccagcgcg cctttgccgt tcatcgtcag cttgccgtca atggcgagcg
      181 gttttttcag gtcgtcggtg acctgcttga cgcccaactg tagcggcgca atgtgcagtt
      241 ttaccgggcg cggcgtggat tcgtctgtga agtcggcgct gctgtcgttg agcgacacct
      301 gggcgatttg ataacgccac gccggacccg cctcattcgc ctttgcgacc ttgtgcgtcg
      361 cgctggcttc cggcgcggtt tcgtgcggac cggcgagcgc ggcgagatcg atactgccgt
      421 ccttcaggcg ttttgcatcg atggaaagac cgttcagcac gacgctgtcc agcgctgccg
      481 tgcgcgccgc gacatcgatc ttccggatct tcgcttgcgc cgacgccagt gcgacagggg
      541 caatgttcgc cgcgttgttc ccctgcggta cgagcttcag cgacttgagc gtgacatcgc
      601 cggcgccgac ctggatggac ggttcgggct tcgaccagtc cacttgcagc ggcagatttg
      661 cgccgagcga accgtcggtg atacgcgcgg ccaccgcgtt cgcgacgtaa ggttgcaacg
      721 gcgccagcgg caaatcgctc agcgcgagtt ttgcgtcggc gttcttgccc ggcagcgtga
      781 aactacccga tgcgtcgagg ccgccgccat gatcgaaggc cgtcttcagc gtgtagcgcg
      841 ccgggggttt gccgagcgtg gagaagtcgt cgagggtcac gttcaaagcg gtcaatccga
      901 gcgtggcgga ttgcgtcatc aagtggtcat cgaagacgat atggccctgg tcaatcgcca
      961 gatgtttgat cgcgaggtcg agcggttgcg ctgcggtggt gtcggtgtcg gtgggcttgg
     1021 cagcgctggc gggagtggcc acagctgttg aagcaggcgt tgttgcgctg agtttttgga
     1081 agttgaatgc gccggtttta tcgcgggaga gtttcacgtc cggctgcgcc aatcgcactt
     1141 catccagtcg atacacattt cgcagcggct cgagattcgc cgcattcacg tgcaactttt
     1201 gtgcggcaaa caacggcgcg tcctgcttgt ccgtcacggc cagatctgcg agatccactg
     1261 tccctttgac gcgaaccacc ggcgtgtcgg cgctgagctc aaagctcaca tcaagattcg
     1321 tcgacacgcg tccgctcttg atcgcaaccg gcagcgaagc gggtgcatac gacgccaatt
     1381 gcggtacgtc gaggccatcg agttgcagcg tgatctccga ctcgcgcgac tcggagaacg
     1441 gctttgtgcg cccgtggaca gacagcggcg aaccgtcgat acgcgcctgc agcgaaggcg
     1501 tgacgaacaa ttccgcggtg gaggcgagat tcgcaataaa cggaatcccg atcgaccagc
     1561 gatcgattat gtgatgcgtt ttaagcaggc ggtcgtcgaa atcgatccgg ccattttcga
     1621 cccgaatatt cgataccgag aatcgcgccg gtttggtgga tggattggca gaaggcttcg
     1681 agaacttttc gacgagatcg gaaaaattaa agcgttgcgc gtcgaagcgc acgatatgaa
     1741 agcgcggcga atcgatttta agttcgttga taatcggcgc gagacgaaac agcgacgacc
     1801 atgaagggcg cacgatcagg cgttctatat cgacgaaatc gccgcttttt gcgtctgcgc
     1861 ccaggtttgc gggcgcttcg ccaatatgca cgcggtcagc ttcgacactc agcgtatagg
     1921 gattaagcgc gattcgttcg atgctggccg ggcgatcgag ttgtgccgag agttgttgtt
     1981 cggcgatatg tcgaatgagc ggaggcgcgg cgaaaaaacc gagccggcca ataacgataa
     2041 cagcgagcag cagcccgatc acaatgcgcc gggtgcgtcg ggcttgcact accgagtttg
     2101 cccctttgag caggctacgc ccggcggatg tcagggaagt cttgttcagg cttgccatgc
     2161 gtgcctcgta gcgcggctgc gcacgcgggc acgagccacg cgcagccttg gggaaacagg
     2221 aggcctgagt atacgacgct gcgctggcaa attgaacccc aagaggggag gtcgttggca
     2281 cttgggggtt tggagggggc tacgttctgg cgttagcggt cggagttagt gccgggttgc
     2341 caggttccag cgttagcggt cggggtcatt gccgggttgc tactttcggc cttggcggtc
     2401 tgcgtgcctt agctgttttc tctatcacta ttagccactg tgcgtggcgg cacgtcattt
     2461 cttggtcctt agcgacaaag aaacgaagca aagaaaacgc ttttaaccac gctaccctaa
     2521 gtgtccacag cgtgcagttc ccattcatag gtgccccaaa agcacggtgc tcgccagagc
     2581 ggaggatgtg tgaaaccctc cttctcgcga atcctgacac gaacacgctt cgccccgtac
     2641 gctctcgggc aagcaccagt gttccacgga ccggcacggc ctaacgcatt tttgagacct
     2701 tgcttcttcg
//
LOCUS       DATASH010000860         1665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_343148,
            whole genome shotgun sequence.
ACCESSION   DATASH010000860 DATASH010000000
VERSION     DATASH010000860.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343148"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /gene="aroA"
                     /locus_tag="VHV99_23025"
     CDS             complement(<1..1028)
                     /gene="aroA"
                     /locus_tag="VHV99_23025"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018435646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX3638295.1"
                     /translation="MEFLDLGPFSRASGTVRLPGSKSISNRVLLLAALAEGETTITNL
                     LDSDDTRVMLDALEKLGVRLTRGGDTCVVTGTRGAFTARSADLFLGNAGTAVRPLTAA
                     LAVNGGDYRIHGVPRMHERPIGDLVDGLRQIGAKIDYEENEGYPPLRIRPAQIKADAP
                     IRVRGDVSSQFLTSLLMTLPLLRTESGVTTVQVDGELISKPYIEITIKLMERFGIKVE
                     RLEWHRFIVPAGQRYRSPGTIMVEGDASSASYFLAAGALGGGPLKVEGVGRSSIQGDV
                     GFADALIKMGANLQMGDDWIEVRGVGNDHGKLDPIDMDFNLIPDAAMTIAVAALFADG
                     TTTLRNIA"
     gene            complement(1047..>1665)
                     /locus_tag="VHV99_23030"
     CDS             complement(1047..>1665)
                     /locus_tag="VHV99_23030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEX3638296.1"
                     /translation="DAGSTKSDVVAAARAALGVRIGQFVPGHPIAGREASGPDAALPD
                     LYVNRNVVLCPLPENAPEAVERVAAMWRATGACVRDMTPGQHDRVLASVSHLPHVLSF
                     ALVEQILNSPDAELKFSFAAGGFRDFTRIAASSPEMWRDVCVANRVALLDELDAYTAV
                     LARLRAAIDAGDGAALEAVFARSRIARSEWQEQRTAGTALGDASK"
BASE COUNT          299 a          568 c          524 g          274 t
ORIGIN      
        1 ctggcgatgt tgcgcagcgt ggtcgtgccg tccgcgaaca gcgccgcgac ggcgatcgtc
       61 atcgcggcgt ccggaatcag gttgaagtcc atgtcgatcg gatcgagctt gccgtggtcg
      121 ttgccgacgc cgcgcacttc gatccagtcg tcgcccattt gcaggttcgc gcccatcttg
      181 atcagcgcat cggcaaagcc gacgtcgccc tggatgctgg accggccgac gccctccact
      241 ttcagcgggc cgccgccgag cgcgccggcc gcgaggaaat acgacgccga cgacgcatcg
      301 ccctccacca tgatcgtgcc cggcgaccga tagcgctgcc cggccggcac gatgaagcgg
      361 tgccattcga ggcgctcgac cttgatgccg aaacgttcca tcagcttgat cgtgatctcg
      421 atgtacggct tcgagatcag ttcgccgtcg acctgcaccg tcgtcacacc gctttcggtg
      481 cgcagcagcg gcaacgtcat gagtagcgaa gtcaggaact ggctcgacac gtcgccacgc
      541 acgcggatcg gcgcgtcggc cttgatttgc gccggacgaa tgcgcagcgg cggatagcct
      601 tcattctctt catagtcgat cttcgcgccg atctgccgca agccgtcgac cagatcgcca
      661 atgggccgct catgcatacg cggcacgccg tggatgcgat agtcgccgcc attcaccgca
      721 agcgccgcgg tcagcggacg cacggccgtg ccggcgttgc caaggaagag atcggcgcta
      781 cgcgccgtga acgcgccacg cgtgccagtc acgacacagg tgtcgcctcc acgcgtcagg
      841 cgcacgccga gcttttcaag cgcgtcgagc atcacgcgag tgtcgtcgga gtcgagcagg
      901 ttcgtgatcg tcgtttcgcc ttcggcgagt gccgcgagca gcagcacgcg attcgaaatg
      961 cttttcgaac cgggcaaacg aaccgtgccg gacgcacgag aaaaaggtcc gagatcgagg
     1021 aattccatga ttgagtccaa ttccggttat ttcgaagcgt cgccgagggc ggtcccggca
     1081 gtgcgctgtt cctgccattc gctgcgcgca atgcgcgagc gcgcgaatac ggcttcgagc
     1141 gcggcgccgt ctccggcgtc gatcgccgcc cgcaagcgcg cgagcaccgc cgtgtaggca
     1201 tccagttcgt cgagcagcgc aacccgattt gccacgcaca cgtcgcgcca catttccggg
     1261 ctcgacgcgg cgatacgcgt gaagtcacgg aagccgccag cggcgaatga gaatttcaac
     1321 tcagcgtcgg gcgaattgag aatctgctcg accagcgcga aagacaaaac gtgcggcaaa
     1381 tggctgaccg atgcgagcac tcggtcatgt tgcccgggcg tcatgtcccg cacgcacgcg
     1441 ccggtagcac gccacatcgc ggccacgcgc tcgaccgctt ccggcgcgtt ctccggcagc
     1501 ggacacaaca cgacgttgcg gttcacgtac aagtcgggca acgccgcgtc cgggccactc
     1561 gcctcacggc cggcaatcgg atgccccggc acgaactggc cgatgcgcac acccagcgcc
     1621 gcgcgtgccg cagcgaccac gtcggacttg gtgctgccgg catcg
//
LOCUS       DATASH010000861         1840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4100398,
            whole genome shotgun sequence.
ACCESSION   DATASH010000861 DATASH010000000
VERSION     DATASH010000861.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4100398"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1446)
                     /gene="pnp"
                     /locus_tag="VHV99_23035"
     CDS             complement(<1..1446)
                     /gene="pnp"
                     /locus_tag="VHV99_23035"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011657486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX3638297.1"
                     /translation="MTMFNKIVKEFQWGQHKVRLETGEVARQASGAVIVDVEDTVVLA
                     TVVGAKTAKPGQDFFPLTVDYLEKTYAAGKIPGGFFRREGRPSEGETLTSRLIDRPLR
                     PLLPEGFYNEVQVVIHVLSLNPEIPADIPALIGASAALAVSGLPFNGPVGAARVAYIN
                     NEYVLNPTRPQMKESALDLIVAGTERAVLMVESEAQQLTEEVMLGAVVFGHEQMQIAI
                     NAIHDLVSEGGKPEWDWQPAAKNEPLIARVTELAQAELLAAYQIRDKQARSTKLKEVY
                     AATSKALEEEAAAAGTVAADKASVGNVLFDIEAKIVRTQILNGEPRIDGRDTRTVRPI
                     EIRTGVLPRTHGSALFTRGETQALVVATLGTKGDEQNIDALEGEYRERFMLHYNMPPF
                     ATGETGRVGSPKRREIGHGRLAKRALAACLPSADEFGYSIRVVSEITESNGSSSMASV
                     CGGCLALMDAGVPMKAHVAGIAMGLILEDNKF"
BASE COUNT          317 a          582 c          553 g          388 t
ORIGIN      
        1 aaacttgttg tcttccagga tcaggcccat cgcgatgccg gcgacgtgcg ccttcatcgg
       61 cacgccggcg tccatcagtg cgaggcagcc gccgcacacc gaagccatcg acgatgaacc
      121 gttcgattcg gtgatttccg acacgacgcg gatcgaatag ccgaattcgt cggcgctcgg
      181 caggcacgca gccagcgcgc gcttggccag acggccgtga ccgatttcac ggcgcttcgg
      241 cgaaccgacg cggcccgttt cgccggtcgc aaacgggggc atgttgtagt ggagcatgaa
      301 gcgttcgcgg tactcgcctt cgagcgcgtc gatgttctgc tcgtcgccct tcgtgcccag
      361 cgttgcaacc accagcgctt gcgtttcgcc gcgcgtgaac agtgccgaac cgtgcgtacg
      421 cggcagcacg ccggtacgga tttcgatcgg gcgcaccgtg cgcgtgtcgc ggccgtcgat
      481 acgcggttcg ccgttcagga tctgcgtgcg gacgatcttc gcttcgatat cgaacagcac
      541 gttgccaacg ctagccttgt ccgccgcaac cgtgccggct gccgctgctt cttcttccag
      601 cgccttcgac gtggctgcgt agacttcctt cagcttggtc gaacgggctt gcttgtcgcg
      661 aatctggtaa gcggcgagca gctcggcttg tgccagttcc gtcacgcgcg cgatcagcgg
      721 ttcgttcttc gctgccggct gccaatccca ttccggcttg ccgccttcgc taaccagatc
      781 gtggatcgcg ttgatggcga tctgcatttg ttcgtggccg aacaccacgg cgccgagcat
      841 cacttcttcg gtcagctgtt gtgcttccga ttccaccatc agcaccgcgc gttccgtacc
      901 cgcgacgatc aggtcgagcg ccgattcctt catttgcgga cgcgtcgggt tcaacacgta
      961 ttcgttgttg atgtaagcca cgcgtgctgc gccgaccggg ccgttgaacg gcagaccgga
     1021 gacggcgagc gccgccgacg cgccgatcag cgcggggatg tcagcgggga tttccgggtt
     1081 cagcgacagg acgtggatca cgacctggac ttcgttgtag aagccttccg ggagcagcgg
     1141 gcgcagcggt cggtcgatca ggcgcgaggt cagcgtttcg ccttccgacg gacggccttc
     1201 gcggcggaag aaaccaccgg ggatcttgcc tgcagcgtag gtcttttcga ggtaatcgac
     1261 ggtcagcggg aagaagtcct ggcccggctt ggcggtcttt gcgccgacca cagtagccag
     1321 gacaacggtg tcttcgacgt cgacgatcac cgcaccgctt gcctgccgag cgacttcgcc
     1381 cgtttcgagg cggaccttat gctgtcccca ctgaaattct ttgacgatct tattgaacat
     1441 agtcattgct tttcctcatc gtaatgccgc gcggaccaga agcggcgcgg caacgccagg
     1501 accggcgccg catgggaaga gtagtgttat gccattccag agaaccacgc cctacgtcga
     1561 gtacgcgcgc gaaccgctgg aatgacacaa agctctgccc tgaagcccgg ccgtatgtct
     1621 gttttttgct gcctttttta ctgctgtttt cctgctagcc gccgtatgaa ccgttacgct
     1681 accgcggcct tgaggcagtg cgcatcacat gaagcgcacc gtgcaaaaac aaaatgcctg
     1741 tatcagtgga actgacacag gcatcttgct gaacgactgg ccgattactt acgcagaccc
     1801 agcttctcga tcagtgcgcg gtaacgatcc gcgtccttac
//
LOCUS       DATASH010000862         6688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3508547,
            whole genome shotgun sequence.
ACCESSION   DATASH010000862 DATASH010000000
VERSION     DATASH010000862.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6688
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3508547"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..980
                     /locus_tag="VHV99_23040"
     CDS             87..980
                     /locus_tag="VHV99_23040"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3638298.1"
                     /translation="MQAIRLKHRERRPASFYWLALLFGLFVLFLSGPVITIFILSFQG
                     PEGGLTFPMRGVSLHWFAVLRDGVGDIDIWAAFGRSVRLALAVTVLTVLFSVAAGLAF
                     RRRFRGDTAVFYVSVASLIMPSIIVSLGIGLTFRLLDNGVKYLASAWGYQSFADSYTT
                     SMGLFTSALGAHLTWTLPFGLLVMFAVFNRFNPAYEEASRDLGATPWQSFRHVVLPII
                     GPSVVGVAMFGFTLSWDEIARTSQAIGDQNTLPLELQGLTTSVTTPVIYALGTMTTVL
                     SLAVMVLCLLAVRGLRRRRAK"
     gene            1091..2362
                     /locus_tag="VHV99_23045"
     CDS             1091..2362
                     /locus_tag="VHV99_23045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX3638299.1"
                     /translation="MPASQSHPTLNQTTADAAELFGEDELTRRFAPLFARIAEGAVER
                     EQNRELAYAQVEWLREAGYTKLRVPKRFGGEGITLKAFLALVTRLGEADSNLQQILRV
                     HGGFIESLLENGDESLRDRWLTRIAQGQIIGGAISERTGVTHNSVRLTQANGAWHLDG
                     EKYYTTGTLYADWVDVTAHDGQSDVRVLVKSDTPGLERIDDWDGFGQRLTGSGTARFA
                     HVPVSLDNLYRRYSASEPRRNTLLTAYYQALHIANLAGISRAALRDAVAFTQGKTRTF
                     GIPGESSPRDNPLVHRVVGRLASLAYSTQSISASLASAIDEVSVARQEGRTGEQTYIH
                     LDIQTFQAQQIVIEQTLQAATLLFEVGGASATSETRRFDRYWRNARVLASHNPAIIRE
                     AAIGDFYLNGNAHNERFGVARNPEVAAVHHA"
     gene            2462..2980
                     /locus_tag="VHV99_23050"
     CDS             2462..2980
                     /locus_tag="VHV99_23050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018425120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HEX3638300.1"
                     /translation="MKNLTAYEPPVDEDQLLLKWIRRARESQMSHYDMADLLSARDRS
                     VGWLVTALTAFVGTAVFASLNSTAVSPALRIFVGLVSVAAAVSAALQTFLRYAERGEK
                     HRAAGARYGAVRRRLEAIFAGDADARDGHYLTAIRDELDRLAEDSPNVPPRVFYRTQR
                     TLSDEPRRSRDA"
     gene            4058..4783
                     /locus_tag="VHV99_23055"
     CDS             4058..4783
                     /locus_tag="VHV99_23055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638301.1"
                     /translation="MPGTTVFRSAITNGAQFEEYVNAIGRPLFAVSCGGDDPGAARAA
                     LDEWLFADDIESLMRAFLDWDARGIHMELEQPATNEAEGGGSWSVVGLPAHFNDAASN
                     LNSGLLGVMCKARGADGARQAFLESVFNDAWMYDFVTLRANRYDSFIEEWKSDDDKNL
                     RPDHYRAQKHFGVIADTGELGTDSRSLQYWVYIGVASGSLTAQQLRDTGLTCKGLIGQ
                     ACVINGLGEVERIGNIPIPRPAS"
     gene            complement(4827..5519)
                     /locus_tag="VHV99_23060"
     CDS             complement(4827..5519)
                     /locus_tag="VHV99_23060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018425121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HEX3638302.1"
                     /translation="MNSTPDAVSRPSIRALTLLEGRILGVLVEKQHTVPDSYPLSLNA
                     LTLGCNQKTGRAPVMNATEAEVLTAVDGLKRLSLVMEGSSSRVPRFEHNMNRVLGLPS
                     QSAALLTTLLLRGPQTAAELRLNSARLHGFADISSVEAFLDELAVNDPPRVVKLARTP
                     GERENRWTHLLCGEVSASDLAQPGGEDEVVPLSAFEAVKAEQKRLADEVSRLQAVVRR
                     MAAELGIEVGEL"
     gene            5971..6549
                     /locus_tag="VHV99_23065"
     CDS             5971..6549
                     /locus_tag="VHV99_23065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEX3638303.1"
                     /translation="MENTLHDDNVNITDRRIAQRLRGLRAERNWSLDDLARLSNVSRA
                     TLSRLENAAVSPTANVLGKLCVAYGLPMSRLMRMVEEDFAPLVPRDAQAVWTDASIGF
                     RRRSVSPPAQALSGEALECELEAGVRIDYDASPRAGLEHHLILLEGQLQITVDGRSHE
                     LQAGDCLRYQLFGPSAFVTPAHCAARYILFIV"
BASE COUNT         1295 a         2026 c         2000 g         1367 t
ORIGIN      
        1 gctaacgcga tcattctgct ggctgtcgtg ttgatgatcg tgtggggtct gacgcgggcg
       61 gtcgatatcc gcaaggagct gtgaacatgc aggccattcg ccttaaacat cgggagcgtc
      121 gaccggccag cttttactgg ctcgcgctgc tgttcggcct gttcgtgctg ttcctgtctg
      181 gaccggtcat tacgattttc attctttcgt ttcagggacc cgagggtggg ctgacgtttc
      241 cgatgcgtgg cgtgtcgttg cattggtttg ccgtgctgcg tgacggcgtt ggcgatatcg
      301 atatctgggc ggcattcggg cgatcggtgc ggcttgcgct ggctgtgact gtgctgacgg
      361 tgctgttctc cgtggcggcc ggattggctt ttcgccggcg ctttcgtgga gatacggcgg
      421 tcttctacgt gtcggttgcc agtctgatca tgccgtccat tatcgtctcg cttgggatcg
      481 ggttgacgtt ccggttgctc gataacggcg tgaagtatct cgcttccgcg tgggggtatc
      541 agtcctttgc cgacagctat acgacatcga tgggcttgtt cacgtcggcg ctgggcgcgc
      601 atttgacgtg gacgctgccg ttcggtctgc tggtgatgtt tgctgtgttc aaccgcttca
      661 atcctgctta tgaagaggct tcgcgagatc tgggcgccac gccgtggcag agcttccggc
      721 atgttgtttt gcctatcatc ggaccttcgg ttgttggcgt cgcgatgttc gggttcacgc
      781 tttcatggga tgagattgct cgcacctcgc aggcgattgg cgatcagaat actttgccgc
      841 tcgagttgca agggcttacc acgtcggtga cgacgcccgt gatttatgcg cttggaacga
      901 tgacgacggt tttgtcgctt gctgtgatgg ttttgtgttt gctcgcggta cgagggttga
      961 ggcgtcggcg ggcaaaatag ccatttcgct acgcgttcta agaatcagcc aaatacttgg
     1021 ttgctagcac cctctgcgtc ccgtaccgtt ctctgattgc gcccggcgcc cgccccgctt
     1081 tggagaactc atgccagcat ctcaatcgca cccaaccctg aatcagacaa ccgcagacgc
     1141 agcagaactg ttcggtgaag acgaactaac ccgccgcttc gcccccctat tcgcccgcat
     1201 cgccgaaggc gccgtggaac gcgaacagaa tcgggaactg gcttacgcgc aggtggaatg
     1261 gttgcgtgaa gccggctaca ccaaattgcg cgtgccaaag cgcttcggcg gcgaaggcat
     1321 cacgctgaaa gcgttcctgg cgttagtcac gcgtctcggc gaggcagact cgaaccttca
     1381 gcaaatcctc cgcgtgcacg gcggctttat cgaatcgctg ctggaaaatg gcgatgagtc
     1441 attgcgtgac cgttggctca cacgtatcgc tcaaggccag atcatcggcg gcgccatatc
     1501 cgaacgcacc ggcgtcaccc ataacagcgt gcgtctcacg caagccaacg gcgcgtggca
     1561 tctcgatggc gagaagtact acaccaccgg cacgctctac gccgactggg tcgacgtcac
     1621 cgcacacgac ggacaaagcg acgtgcgcgt gctcgtcaag agcgacacgc cgggcctcga
     1681 gcgcatcgac gattgggatg gcttcgggca gcgtctcacc ggcagcggca cggcgcgttt
     1741 cgcgcatgtg ccggtctcgc tcgacaatct gtaccgccgc tatagcgcct ccgaacctcg
     1801 ccgcaacacg ctgcttaccg cgtactacca ggcgctgcat atcgccaacc tggcgggtat
     1861 cagccgtgcg gcgctgcgcg atgcggtggc gttcacgcaa ggtaaaactc gaaccttcgg
     1921 tatcccgggt gaatcgagcc cgcgcgacaa tccgctggtg catcgcgtag tcggccggct
     1981 cgcgagcctt gcttactcga cgcagagcat cagcgcttcg ctagccagcg cgatcgatga
     2041 agtgtcagtc gcgcggcagg aaggccgcac tggcgaacag acctacatcc atctggatat
     2101 ccagaccttc caggcgcaac agatcgtcat cgaacaaacc ctgcaagcgg cgaccttgtt
     2161 gttcgaagtg ggcggcgctt cggcaaccag cgaaacgcgc cgcttcgacc gctattggcg
     2221 caatgcgcgg gtgctggcgt cgcacaatcc ggcgatcatc cgcgaagccg ctatcggcga
     2281 cttctatctg aacggcaacg cgcataacga acggttcggt gtggcgcgca atccggaagt
     2341 cgccgccgtt caccacgcct gaaaagcaaa accccagcgt tcagctcgcc gccctcatgt
     2401 ggcacagtat ggtcaggcgc atgcgccacg catgcgccgc tggacagccc tgacgagcga
     2461 catgaagaac ctgactgcgt acgagcctcc ggtcgacgaa gaccagcttc tgctgaaatg
     2521 gatccggcgc gcgcgcgaat cccagatgag ccattacgac atggccgacc tgctctcggc
     2581 gcgagaccgc agcgtgggtt ggctcgtcac cgcgttgacg gcgtttgtgg gcacggcggt
     2641 gtttgcgtca ttgaattcga cggccgtctc gccggcgctc agaattttcg tgggcctcgt
     2701 gagcgttgcg gcggcggtct cagccgcgct gcaaaccttc ctcagatatg cggagcgcgg
     2761 cgaaaagcat cgcgcggcgg gtgcgcgcta cggcgcggtg cggcgcaggc tggaggcgat
     2821 cttcgcaggc gacgccgatg cgcgtgacgg ccactatctc accgcgatcc gcgacgaact
     2881 cgaccggctc gccgaggatt cgccgaacgt gccgccgcgc gtcttctatc gcacccagcg
     2941 aaccctgtcc gacgaaccgc gacgcagccg cgacgcgtga gcggccgccg ttttgcctga
     3001 aaactatcgc atagcggata tcggcaacat cattggccga tcgcgttctt ccgccaactg
     3061 tagttgccga agaatgttgg gtcgtcgtaa atgttcaggg tcatctgtaa atagcattag
     3121 ccgtaattga atgcaacatg gccgcatcta taaagccggt tttggaccgt cttcatcgtt
     3181 tttaccacgc gaggtaaacg gttcaatcgc ccagacaggt ctggcttgct aaaagcaact
     3241 gattttcctg agcctgacct tcggacgaag aatgcatttc cactgactca agccagacgg
     3301 catgatctga aatcgatggc gccggtatct atgtagttaa aaatcgctta ataagcatga
     3361 cctgccaacc gtgcaaacga tctgttcctc ctggtcggaa gactgcatga aaacacaggc
     3421 tacactcgag cgtatagcgc ttcaagaatt cgcatgcatt acgcctggaa tagtttaatg
     3481 atgaaacgtg acatggcagc cgaacaggga tatgcacaaa taatggggaa gctataactt
     3541 tcccgtcatg tattgctatt aagcaggggg acgccttgtc tcctcgcggc tatcgatgcg
     3601 gggtttcgcg cagcaatacc gccgataccc ataaccacaa cgaccccgcc accctcacgg
     3661 cgatcgggaa gcagcgcccg atattcttca aaaaaaaagc ccgccaccgg cgggcataat
     3721 caccattcac ggctcgggga agccgcagtg acactcaggg aaccatcgaa ctgcatgaaa
     3781 atcttagctc attatcggat gaggctgcga gcaatttttc ataaaatcga aaataaaaat
     3841 acctatccgg catggcttat ttttcacttg ccgcagttta ttataatttt cagcaacaca
     3901 atttaagcat ttatgcaaga gtctcgacca attaagcatt tgccaaaatt tagcgcaccc
     3961 ttctcgccgt gataaaactt tagtattgtg tcggttcgcg cgtttcccaa taaaaaaatc
     4021 ccgtaacgca tggaggttat aaatgacgaa agctcccttg cccggcacaa cggtcttcag
     4081 gtcggccatt acaaatggcg cgcaatttga agaatacgtc aatgctattg ggcgcccgct
     4141 attcgcagtc agttgtgggg gcgatgatcc gggcgcggcg cgggcggcgc tggatgagtg
     4201 gctttttgcc gacgatattg aatctctgat gcgggcattt ctggattggg atgcgcgcgg
     4261 tatccacatg gaactggaac agccggcaac gaacgaagca gaaggtggtg ggtcctggag
     4321 cgtcgtggga ttgccggctc atttcaacga tgcagcgtcg aacttgaata gcggcttgct
     4381 cggtgtgatg tgcaaggcga ggggcgctga cggcgcccgc caggcgttcc tcgaatcggt
     4441 gttcaatgac gcgtggatgt acgatttcgt tacgctccgc gcgaaccggt acgacagctt
     4501 catcgaggaa tggaaatccg acgacgacaa gaatttgcgg ccggaccact atcgggcaca
     4561 gaagcatttc ggggtgatcg ccgataccgg cgaactgggt accgacagcc ggtcgctgca
     4621 gtactgggtc tatatcggcg tggcgagcgg cagcctgacg gcccagcagc tcagggatac
     4681 cggcctgacc tgcaaagggc tgatcggtca ggcgtgcgtg atcaatggtc tcggcgaggt
     4741 cgaacggatc ggcaatatac cgattccgcg ccctgccagt tgagctttcg gctgcggccc
     4801 cgcgttcggt tgagtggggt gttcgctcaa agctccccca cttcaatccc caactccgcg
     4861 gccatgcgcc gcactaccgc ctgaagccgg ctcacctcat cggcgagccg cttctgctcg
     4921 gctttcaccg cctcgaacgc cgacagcggc acgacctcat cttcaccgcc cggctgtgca
     4981 agatcacttg cgctgacttc gccgcagagc agatgcgtcc agcggttttc gcgctcgccc
     5041 ggcgtacggg ccagcttcac cactcgcggc ggatcgttga cagccagttc gtcgagaaac
     5101 gcttcgactg atgagatatc ggcaaatcca tgcaagcgcg cgctgttcaa gcgcaactca
     5161 gccgcagtct gcggcccgcg caaaagcaaa gtagtcagca acgcagccga ctgactcggc
     5221 aaccccaaca cacggttcat gttgtgctcg aagcgcggca cgcggctgct gctgccctcc
     5281 atcaccaggc tcaggcgctt caatccgtcg acggcggtca gcacctcggc ttcggtcgcg
     5341 ttcatcacgg gtgcgcggcc ggtcttctga ttgcagccca aggtcagcgc gttcagcgaa
     5401 agtggataac tgtcgggcac ggtgtgctgt ttttcgacaa gcacgccgag gatgcgcccc
     5461 tcaagcaagg tcaaagcgcg gatggacggg cgggaaacag cgtcgggagt ggagttcatg
     5521 gaaaatgtcg cggcatgcgc cgtcaggtct cgtgagtggt gtgctttgcc gctgtaccgc
     5581 ggcgttgcac agccgcgttg cacagccggg cggcgggttt tagtttgtgg gcactatgat
     5641 aaacgccggg cggctcgcgg tgtatgaatc ccggcaatgg cgagggcgcg gccacttgct
     5701 cgtggccccg gcagtgacgg aatgcagcca cccgtccttg gccccggcag tgacggaatg
     5761 cagccaccca cgccctggcc ccggcagtga cggaacacgg ccacccgcgc ctggccccgc
     5821 cagcgacgga acacggccac ccgcgccagg cctctgccgt ggcagaacgc ggtcacgcgc
     5881 cgccggcccc ggcagcgaca gaacgcggtc ctcaccaggc gcatttttgt ttcattgacg
     5941 attttccaca ttcgagataa tttcccggat atggaaaaca cgctccacga cgacaacgtc
     6001 aacatcaccg accgccgcat cgcccagcgt ctgcgcggat tgcgcgccga gcgcaactgg
     6061 tcgctcgacg atctcgccag gctcagcaac gtcagccgcg ccaccctgtc gcgcctcgaa
     6121 aatgccgccg tgagcccgac ggcgaacgtg ctcggcaagc tctgcgtcgc ctacggcttg
     6181 ccgatgtcgc gcctcatgcg catggtcgag gaagacttcg caccgctggt gccgcgcgac
     6241 gcgcaggcgg tctggaccga cgcgagtatc ggcttccggc gccgctccgt gtcgccgccg
     6301 gcccaggcgt tgagcggcga ggcgctggag tgcgaactcg aggccggcgt gcggatcgat
     6361 tacgacgcgt cgccgcgagc cggtctcgag catcatctga tcctgctcga agggcaattg
     6421 cagatcaccg tcgacggcag aagccacgag ttgcaagccg gcgactgtct gcgctatcag
     6481 ttgttcggcc ccagcgcgtt cgtcacgccg gcgcactgcg cggcccgcta cattctcttc
     6541 atcgtctgag ttcgaaccga catgagcgcg accaccatca cgaccctctc cgccgaggac
     6601 ctcgttcgcc atttatccga actcggcgcg ctgctgcgtg cctgcgtcca cgatggcgcg
     6661 agtgtcggat ttgtcctgcc gtatggtg
//
LOCUS       DATASH010000863         1984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3113356,
            whole genome shotgun sequence.
ACCESSION   DATASH010000863 DATASH010000000
VERSION     DATASH010000863.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3113356"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="VHV99_23070"
     CDS             <1..814
                     /locus_tag="VHV99_23070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006052247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEX3638304.1"
                     /translation="GHAGQDALNECMREHGGFEHNLQSLAVVDDLEEHYGAFNGLNLC
                     FETREGILKHCSRENARRLGALGERFMEGRQPSIEAQIANVADEIAYNNHDVDDGLRS
                     GLLTVEQLAEVALWQTHYEAARGDFPQIEGRRLIHETVRRIINTLIVDLIDTTLLNLA
                     RHAPVSLDDVRAAPPLVAHSEAIAAQAAALKRFLFKNLYRHYRVMRMANKARRVVAGL
                     FDAFTEDPRLLPPSYQSTDASLQPRLIAHYIAGMTDRYAVKEYQRLFVIDDN"
     gene            complement(949..1329)
                     /locus_tag="VHV99_23075"
     CDS             complement(949..1329)
                     /locus_tag="VHV99_23075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HEX3638305.1"
                     /translation="MTALPNTRDALGESWTTTSRRARRIARHSRHAAEDIAGELRNLM
                     SELESTLADGTQADAVALRGKLRKQLDAARARLNDTREAVRERAEVAIADADDYVHEN
                     PWQTVAIVGGVALIAGALFAARLR"
     gene            complement(1667..>1984)
                     /locus_tag="VHV99_23080"
     CDS             complement(1667..>1984)
                     /locus_tag="VHV99_23080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HEX3638306.1"
                     /translation="YLGVGVSRIWFTDEKITNGAFEASVLHGPTSVSTDSSWEPVFNA
                     GFTYAFNQHWFAGVSISYLPLSTTAKLNTAANTPAGTLTVQSQTKIKLNPIVTYVNIG
                     YRF"
BASE COUNT          394 a          613 c          618 g          359 t
ORIGIN      
        1 cggtcacgcg ggccaggacg cgctgaacga atgcatgcgc gaacatggcg gcttcgagca
       61 caacctgcaa agcctcgcgg tggtcgacga tctggaagag cactacggcg cgttcaatgg
      121 gctgaacctt tgcttcgaaa cgcgcgaggg cattcttaag cactgctcgc gcgagaacgc
      181 gcggcggctc ggcgcgctcg gcgagcgttt tatggagggg cggcagcctt ccatagaggc
      241 gcagatcgcc aacgtcgccg atgagatcgc gtacaacaat cacgatgtcg acgacggctt
      301 gcgttcgggc ttactcacgg tggagcagct tgccgaagtg gcgttatggc agacccacta
      361 cgaggcggcg cgtggcgatt tcccgcaaat cgaagggcga cggctgattc acgagacggt
      421 gcgccgtatc atcaacacgt tgatcgtcga cctgatcgat acgaccttgc tcaatcttgc
      481 aaggcacgcg ccggtctcgc tcgacgatgt gcgggccgcg ccgccgctgg tcgcgcacag
      541 cgaagcgatc gccgcgcagg ctgcggcgct caagcgcttt ctgttcaaga acctgtatcg
      601 acactatcgc gtcatgcgca tggctaacaa ggcgcgccgc gtggtggccg ggctgttcga
      661 tgcgttcacc gaagatccaa ggctattgcc acccagctat caatcgacgg acgcatcgtt
      721 gcaaccgcgg ctgattgccc actacatcgc gggtatgacg gatcgctatg cggtgaagga
      781 ataccagcgg ctgtttgtga tcgacgacaa ctgagcggac caagcggcga aacccggcgc
      841 cagtacccgg cgccggtacg gcggcatgat tcgcaacgcg agtcgccggc cgcggcgacc
      901 cgccagttcg ctattgcgcg ctaacgtagg gcgcttaccc caacgtgcct agcgcagccg
      961 cgccgcgaac agcgcacccg cgatcagcgc aacgccgccg acgatcgcca ccgtctgcca
     1021 cggattctca tgcacgtaat cgtcggcatc ggcgatcgcg acttcggcgc gttcgcgaac
     1081 ggcttcgcgg gtgtcgttca aacgggcacg cgcggcgtcg agctgcttac gtaatttgcc
     1141 gcgcagcgca acggcgtctg cctgggtgcc gtctgccaga gtggattcca gctccgacat
     1201 caagttgcgc agttcgccgg cgatatcttc ggccgcatgg cggctatggc gagcgatacg
     1261 ccgtgcacgg cggctggtgg tggtccagga ttcgccgagg gcgtctcgcg tgttcggaag
     1321 tgctgtcatg gtcgctccgt cgatgagggt gaagggtccc cacgctgtca cgcagacaca
     1381 gttgtcgcgc gggaaacacc ctgagaacgc aacttcggtg ccaaacccgc agcccctgtc
     1441 caccgtggca ccgccccaca ttcctcagca gatattgcac gagccggccg gatgatgggt
     1501 caaatttaca aacgctcccg cccgcgcgct gaagtgggct ccacacgccg caaaccgtct
     1561 cgcgacgcgc gcgaattaaa aaacgccgtc attgaatgca tgacggcgtt gtccaagcca
     1621 cgaaaattcg actaccctaa cttaacggcc ccaatttcgt gacgcgttag aaacggtaac
     1681 cgatgttgac gtaggtaacg atcgggttca gcttgatctt ggtttgcgat tgcacagtca
     1741 gcgtaccggc cggggtgttg gcggcggtgt tcagtttggc agtcgtgctg agcgggaggt
     1801 acgagatcga gacgcccgcg aaccagtgct ggttgaacgc ataggtgaaa cccgcattaa
     1861 acaccggctc ccacgaacta tccgtggaga cgctggtcgg accatgcaga acactcgcct
     1921 cgaacgcgcc attggtgatc ttttcgtcgg tgaaccagat gcggctcaca ccaacgccga
     1981 gata
//
LOCUS       DATASH010000864        16515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_542278,
            whole genome shotgun sequence.
ACCESSION   DATASH010000864 DATASH010000000
VERSION     DATASH010000864.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 16515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16515
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542278"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VHV99_23085"
     CDS             complement(<1..458)
                     /locus_tag="VHV99_23085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HEX3638307.1"
                     /translation="MFASAQARAEALTVTDDTGATVTLAAPAQRIVSLAPHVAELLFA
                     AGGGARVVGAVSYSDYPPQAKAIPRVGDNKALDLERIVAMKPDLIVVWRHGNAMRQID
                     RLRELHVPLFFSEPHRLDDIPVTIDKLGILLGTSGEANKASDAFRHDIAS"
     gene            complement(618..1625)
                     /gene="cobD"
                     /locus_tag="VHV99_23090"
     CDS             complement(618..1625)
                     /gene="cobD"
                     /locus_tag="VHV99_23090"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase CobD"
                     /protein_id="HEX3638308.1"
                     /translation="MAEPIRHGGNLREAARRYEIALGDWLDLSTGINPRAYQVPPVPM
                     DAWRRLPEDGDGLAELAAHHYGAPAALPVAGSQAAIRALPGVLKRGVAAVAPLTYGEY
                     APAFERAGHRIVTLDIASAALTDDIDHVIVSNPNNPTTERIGRDVLLHWHEQLAARGG
                     TLIVDEAFADAYGDAATSMASESGREGLVVLRSVGKFYGLAGIRAGFVLAAAPLRDAL
                     SHALGAWTVSGPARHAVQAAFNDLSWQAETRATLARDSARLAASVRNQGFDPYATPLF
                     VWFETDKAAALQHALATRGIWTRLFEHGASPSLRIGLTGSDGEWARFEEAFNASVATI
                     R"
     gene            complement(1618..2559)
                     /gene="cbiB"
                     /locus_tag="VHV99_23095"
     CDS             complement(1618..2559)
                     /gene="cbiB"
                     /locus_tag="VHV99_23095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HEX3638309.1"
                     /translation="MLLLSFYVTAMLAIAGVVIDRIFGEPAARHPLVAFGRLATHIET
                     KMNTGFRARPAGILAWLLAVVPPVMVSWILVSVLPFAAACAVHVALLWFSLGARSLRD
                     HIAPIARALGLGDLAAARKLTARIVSRDTTHADEAALSRAAVESALENGNDAIFGALF
                     WFVIAGGPGALMFRLANTLDAMWGYRTPRYLRFGWAAARFDDVLNFVPARLTAASYAL
                     MGDTRTAWRCWRTQAPLWDSPNAGPVMAAGAGSLNVLLGGPAIYNGALETRPVLGFGM
                     TASPRHVVAALRLVERTTLLWLAVLLVLAFTGVGFHG"
     gene            complement(2561..3097)
                     /gene="cobU"
                     /locus_tag="VHV99_23100"
     CDS             complement(2561..3097)
                     /gene="cobU"
                     /locus_tag="VHV99_23100"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007000026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HEX3638310.1"
                     /translation="MTSPDLTFILGGARSGKSAHAERLAADSGLPVTYVATARIGDAE
                     FAARVQQHRERRPAHWALVEAPLDLGGVLRELDREGHVVLIDCLTLWLANLLFPADGG
                     TPDGPPRQAFSDFEAALSTVRGKVIVVSNEIGLGVVPMGSMTRLYVDELGRLNQRVAA
                     ASTHVTMMVAGLPLVLKG"
     gene            3174..4658
                     /locus_tag="VHV99_23105"
     CDS             3174..4658
                     /locus_tag="VHV99_23105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016344571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HEX3638311.1"
                     /translation="MQFAPRGTLMIQGTTSDAGKSTLVAGLCRLARRGGTRVAPFKPQ
                     NMALNSAVTVDGGEIGRAQALQAIAAGVDAEIDFNPVLLKPTSDRGAQVIIHGHAHAN
                     LDARAYHEYKTVAFDAVLASYARLQRRFDAIIVEGAGSPAEVNLRDRDIANMGFAERV
                     DCPVVLVADIDRGGVFAHLLGTLACLSETERARIRGFVINRFRGDISLLQPGLDWLEA
                     QTGKPVLGVVPYLHGLTLDAEDMLPSQLHTRGDAGGEVLKVIVPALPRISNHTDFDAL
                     RAHAQVDFSYVRAGTAPPPADLIVLPGSKSVQRDLAWMRENGWDRAIERHLRYGGKVL
                     GVCGGLQMLGRTVDDPQGFEGPAGCVNGLGLLDLDTTLTADKLLDNVTGRLTLGAGPN
                     PAPVRGYEIHMGRTTGAALARPAVMLDGWRPDGAISADNQIAATYLHGIFDTPEACAA
                     LLEWAGLSNAEALDYPALREASLDRLADTLGQALDLDAIGAMFA"
     gene            complement(4688..5107)
                     /locus_tag="VHV99_23110"
     CDS             complement(4688..5107)
                     /locus_tag="VHV99_23110"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX3638312.1"
                     /translation="MATLRPDITLDSLRERQRGCLPDLLGVEVVSLESGTLTGKLTIR
                     HELLAPNGFLHAATVIGLADTCCGYACIAHLPEGAKNFTTVELKSNHVGTARDGVIAA
                     KATAVHLGRSTHVWDAIVTNGEGKTIALFRCTQMILY"
     gene            5269..5895
                     /locus_tag="VHV99_23115"
     CDS             5269..5895
                     /locus_tag="VHV99_23115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016344573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX3638313.1"
                     /translation="MTVIVVANPKGGVGKSTLSTNLAGYFAANGEWVALADLDKQQSA
                     HGWLGLRPDTLPKIEEWRTNVDAPSKPPRGLEKAVVDTPAGIHGTRLALALELADKVI
                     VPLQPSMFDILATQEFLERLQKEKAIRKGTIEVGVVGMRVDARTKSADQLHRFVEGLD
                     LPVLGFIRDTQNYVQVAAHGLTIWDVAKSRVEKDIEQWQPIIEWVSKK"
     gene            complement(5989..6375)
                     /gene="panD"
                     /locus_tag="VHV99_23120"
     CDS             complement(5989..6375)
                     /gene="panD"
                     /locus_tag="VHV99_23120"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEX3638314.1"
                     /translation="MQRTLLKSKIHRATVTHCELHYEGSCAIDEDLLEAANLVENEQI
                     DIWNVNNGERLTTYAIKGERGSGMISLNGSAARRAQLGDLVIIAAFALVDEAELKAGW
                     KPDLVFVDEKNRIKGNRDHVPTQNWT"
     gene            complement(6398..7231)
                     /gene="panC"
                     /locus_tag="VHV99_23125"
     CDS             complement(6398..7231)
                     /gene="panC"
                     /locus_tag="VHV99_23125"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011352891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX3638315.1"
                     /translation="MKVISSIHELRDQLRGQNRTAFVPTMGNLHEGHLSLMRLARQHG
                     DPVVASIFVNRLQFGPNEDFDKYPRTLQDDIEKLQSENVYVLFAPTEADLYPEPQQYR
                     VHPPTNLGDILEGEFRPGFFQGVCTVVMKLMSCVQPCVAVFGKKDYQQLMIVRQMCHQ
                     FALPTEIIAAETVRDADGLALSSRNRFLQAAERAEAPILAKALNRVRDAVLAGNRDFA
                     SIEKEAVESLAQRGWQPDYIAVRRRVDLLPPGPADTATPLVVLAAAKLGTTRLIDNLE
                     I"
     gene            complement(7340..8125)
                     /locus_tag="VHV99_23130"
     CDS             complement(7340..8125)
                     /locus_tag="VHV99_23130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016344576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HEX3638316.1"
                     /translation="MDGVAFAHLYGEPLWRLPTDLYIPPDALEIFLEAFEGPLDLLLY
                     LIRKQNFNVLDIPMADVTRQYLGYVDQIRESNLELAAEYLLMAAMLIEIKSRMLLPVK
                     KADTGEEAEDPRAELVRRLLEYEQMKLAAQRLDHLPQLGRDFLRAEVYVEQSSAQRFP
                     DVDANDLRAAWADVIKRARLVEHHKISREELSVREHMSTILRRLQNARFMEFGDLFDA
                     SRGVPVVVVNFIAMLELSREALIEITQAEPYAPIYVRLAYSPA"
     gene            complement(8248..8436)
                     /locus_tag="VHV99_23135"
     CDS             complement(8248..8436)
                     /locus_tag="VHV99_23135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017333879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HEX3638317.1"
                     /translation="MMYLSDITQFINQMKEQKPSLEAEQRQGRALLWDKQPIDLDERS
                     EIKTSRVEQTPYVYYQNF"
     gene            9109..10842
                     /locus_tag="VHV99_23140"
     CDS             9109..10842
                     /locus_tag="VHV99_23140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex family protein"
                     /protein_id="HEX3638318.1"
                     /translation="MRRVVWSLHALALFAVAQPAHAKYDVDIDAPRSVRSLLKDHLDL
                     SRFKMRDDISDEQFDFLVTATPQEVRDLVATDGYFTPVVRTDVKHDGDKKSVTISVDP
                     GPQTKVASVSLTFKGAITTEDPAQQNTARLAFSVKEGDPFSQGAWDDAKNAALKALQA
                     RRYLGAKISQSKARVNPRTHIADLSVTFDSGPTFTFGQLDISGVRRYPEKIIHNVNRI
                     NPGDTYDVARVSELQRQLQNTPYYASVAIDADNDVNKPNETPMHLKVSEYPYNSVRYG
                     VGYATDTGPHVQGAYSYLDTFGKAYPFTVSGRLDQTQQYGQVQLAMPPDGRTWVNSVL
                     ASYTNTNVSDTRIYSARVGVQRMRSLQNIDTTFALLLYADRLTQNLGPPTSSRALVPS
                     WQWVRRNVDDPLFPRSGNLIRAEADVAVKGILTDQTFGRVYTNLLQYLPLGKRDLFVF
                     RAEFGGVFTAGSSNGVPASLLFRAGGANSVRGYGYQSIGNNVDGSVLPTKYLMTGSSE
                     YQHWFSHDWGGAVFFDIGTATDTWKERVFEPGTGVGVRWRSPVGPVNVDIAYGFKNRD
                     IRPYLTLGIAF"
     gene            10880..14938
                     /locus_tag="VHV99_23145"
     CDS             10880..14938
                     /locus_tag="VHV99_23145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016344579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEX3638319.1"
                     /translation="MSDQNGQSPADQPDPSEDRGNGTSENGTPVPKPRRGGWGRLAKW
                     LGGIVLVLVLCFALGVAMILYAVNSERGTRYVWQAATSLLNARLSGTLDGGAIATGLR
                     LRNVHWKSLDGNGTDIAVDSVSGKWELTRAPLRFTIGYLHVGTVDARIAPSPKDTSKT
                     ELPTDLRLPIQLAISDVTLDKLRLHEGATTTEFSRLALDGHSDGRHHEARIQRLDTPF
                     GAITAALKLDGGARPFPLTGDAGYSGEVSGEAVQVAAHLTGSLENLAADINASGLKLK
                     GQAHVEAAPFGDVPLKRALVTIDHVNPQAFSPSAPFADLALRAELTPDPATPNALVVT
                     GPISIVNAKPGSIDKQLLPLIDAHANVKLDASAQTISDLNLRLLKDAIVTGGGTLKGG
                     TGQFDLKVAKLDLNAIEPTIRPTQFAGPVGIHLDGDKQTITLDLNDPKAALRAQGKVT
                     LDAKQTAFDGVKLSVGKGRIELSGALQKDIHSSYDLKAKVIDFNPLLLTDELVAKPPP
                     KGSKAKPVAKSRPIEAHVNGTLSAAGVLAPTLTTKAQFKLGDSVYDNLPLTGSGTIQV
                     VGTRILPSTATLSIAGNNVDLNGSFGAPSDRLRFRVDAPQLERLGFGLAGLVKADGDI
                     TGSFAHPNLAANYQVDSVVFGANRVGHAEGRAEARDGANGALVFTLQARDVSTDGVDL
                     ASLDANLNGTRANHTLNATAVGKVRGNAVNLALAANGRLTDARDGAHWDGTVTRLSNK
                     GTPNVNLDAPLTVSYAPNHVALGATRLSAEGAVLDLKSFALDGGRINSAGQLTNLSVA
                     RLLEIRQELEGGPPPARTDLVFDGDWNFALGATATGYVQLKRRSGDVSIDAARGVAAL
                     GITDITARADFTGGNRLNATVHAQATRIGVIDANVHTNLVARDGVLTVADEAPLSGAI
                     EANVPTLRTTGGLFGPAYLLDGRLGLKLTIAGIVAKPTLSGMLTGDALSVTVIDQGVQ
                     LKDGVIRIALSENLVDLQQVEFHGASGTLRATGKVRLDQQQPDLTASIIADKLELFAS
                     PDRQLQLSGSASVANAGLQGGMAIDGKFTIDHALFDLPEQSAPSLGDDVVIVRPDGTV
                     KGEDQKVVATGDKPVGAFAPRANIDINLGQKFRFRGAGADLGLAGTVTAMSAPNMPLR
                     AVGNVRVTPGSTYTAFGRRLGIENGFFTFNGPVANPGINILAMRRNQEIEAGVQVTGT
                     VQSPVAKLISEPNVPDNEKLSWLLFGHGTDQGNNVGQQSAMTNALALLGSSQGKRIAQ
                     TFGLDEFSIGTSEVGLTDPQVVMLSKAINERLVLGYEQGLQSASNAIKATLSLSRFWS
                     ISAYGGTFQGMDLLYTRRFDSWSRRNGPGDSK"
     gene            complement(15051..>16515)
                     /gene="metG"
                     /locus_tag="VHV99_23150"
     CDS             complement(15051..>16515)
                     /gene="metG"
                     /locus_tag="VHV99_23150"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008924243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX3638320.1"
                     /translation="AAPDDAVRAKLDEWLKAGLQDWDISRDAPYFGFEIPDAPGKYFY
                     VWFDAPIGYLASFTQLCARTGLRFDDFFGADSRAELHHFIGKDILYFHTLFWPAMLEF
                     SGHRTPTNVFAHGFLTVDGTKMSKSRGTFITAKSVIDTGINPEWLRYYFAAKLNHTME
                     DIDLNLGDFQARVNSDLVGKYVNIASRAAGFLIKRFEGRVLDSVMNHPLLLQLRGAVP
                     QIAALYEAREYGRALRTTMDLADAVNAFVDTAKPWDQAKDEANAAALHETCSVSLEAF
                     RLLSLALKPVLPVVAESVEGFLGIAPLVWADVSAPLSSSQPINAYKHLMTRVDPKQIE
                     ALIAANRESVAPGQAVAAAPAAASSSSSKKAAKVKEPEAEGVISIDDFAKVDLRIAKI
                     VNCTAVEGSDKLLQLTLDIGEENTRNVFSGIKSAYQPQDLIGKFTVMVANLAPRKMKF
                     GLSEGMVLAASADDEKAEPGLYILEPHSGAKPGMRVK"
BASE COUNT         3123 a         5095 c         5263 g         3034 t
ORIGIN      
        1 aagctggcga tgtcgtgacg gaatgcgtcc gacgccttgt tcgcttcacc cgatgtcccg
       61 agcaggatgc caagtttatc gatggtgaca gggatatcgt ctagcctatg cggctcgctg
      121 aaaaacagcg gcacatgcag ttcgcgcaac cggtcgatct gccgcatcgc attgccgtgc
      181 cgccagacga cgatcagatc gggcttcatc gcgacgatgc gttcgaggtc cagcgctttg
      241 ttatcgccga cacgcgggat ggctttggct tgcggcgggt agtcgctata gctgaccgct
      301 ccgaccacgc gtgcgccgcc gcctgccgcg aacaggagtt cggcgacgtg cggtgcgagg
      361 ctcacaatgc gctgtgcggg agcggcgagg gtgacggtgg cgccggtgtc gtcggtgacc
      421 gtgagagctt cggctcgggc ttgggccgat gcaaacatcg cggcggcggt gaaggtcagc
      481 gtgaggaagt ggcgcaaagt catgaggtgg attggttctt atgcgtcatg gtgggcgagc
      541 gctgtgcagg aagcacgcat actcgctttt gatgggaagc gcggcgggtg gaacaccgga
      601 cgtgaaggct caagcgctta ccggatcgtt gcaacggatg cgttaaacgc ctcttcaaac
      661 ctcgcccatt ccccgtccga tcccgtcaac ccaatccgca aacttggcga cgcgccatgc
      721 tcgaagagcc gcgtccagat cccgcgcgtg gccaaagcgt gttgcagcgc cgccgctttg
      781 tcggtctcga accacacgaa aagcggcgta gcgtaaggat cgaaaccttg attgcggact
      841 gacgctgcca gccttgcact atcgcgagcc agcgtggcgc gagtctctgc ctgccatgaa
      901 agatcattga acgcggcctg caccgcatgt cgtgccggac cgcttactgt ccacgcaccg
      961 agcgcgtgtg aaagcgcgtc gcgcaacggc gccgccgcga gcacaaaccc cgcgcgaatc
     1021 ccggccagcc cataaaactt gccgaccgag cgcagcacga ccagcccctc ccgcccactt
     1081 tccgatgcca ttgacgtggc cgcatcgccg taggcatcgg cgaacgcttc atcgacgatc
     1141 aacgtgccgc cgcgcgccgc caattgctca tgccaatgca gcaggacatc acggccgata
     1201 cgttcggtcg tcggattgtt aggattcgaa acgatcacat gatcgatatc atccgtgagc
     1261 gcagccgatg caatatcgag cgtcacgatc cgatgccccg cgcgctcaaa cgcgggtgcg
     1321 tattcgccat aggtcaacgg cgcaacggcc gcaacgccac gcttcaacac gcccggcagc
     1381 gcacgaattg ccgcctgact gcccgccacc ggcagcgccg ctggcgcgcc atagtgatgc
     1441 gctgcgagct cggcgagacc atcgccgtct tcgggcaaac ggcgccacgc gtccattggc
     1501 acaggcggca cctgatatgc acgcggattg atccccgtcg ataaatccag ccagtcgccc
     1561 agcgcgattt cgtatcgacg tgcagcttcc cgcaaattcc cgccgtgccg gatgggttca
     1621 gccatggaac cccacgcccg tgaaagccag caccagcagc accgcgagcc agagcagcgt
     1681 cgtgcgttcg accagccgca gcgccgccac cacatgccgg ggcgaggcgg tcatcccaaa
     1741 cccgagcacg ggccgcgttt ccagcgcgcc gttatagatc gccgggccgc cgagcaacac
     1801 gttcaggctc cccgcacccg ccgccatcac cggccccgcg ttcggactgt cccacagcgg
     1861 cgcctgcgtg cgccagcagc gccacgccgt gcgggtgtcg cccatcagcg cgtagcttgc
     1921 agccgtcagc cgcgcgggca cgaagttcag gacatcgtcg aaacgggcgg cggcccagcc
     1981 gaaacgcagg taacgcggtg tccgataacc ccacattgcg tcgagggtat tggcgaggcg
     2041 gaacatcagc gcgcctggtc cgcctgcgat cacgaaccag aacagcgcgc cgaaaattgc
     2101 atcgttgccg ttttccagcg cggattccac ggctgcgcgg gacagcgcgg cttcatcggc
     2161 gtgggtggtg tcgcgcgata cgatccgcgc cgtcagcttt cgcgcggcgg ccaggtcgcc
     2221 gagcccgagc gcgcgggcga tcggcgcgat gtgatcgcgc aggctcctgg cgccgagtga
     2281 aaaccacagc agcgcgacat gcactgcgca cgcggcggca aaaggcagca cactgacaag
     2341 aatccagctc accataaccg gcggcacaac ggccagcaac cacgcgagaa tgcccgccgg
     2401 ccgggcgcga aaacccgtgt tcatttttgt ttcgatatgc gtggcaagcc gcccgaacgc
     2461 aaccagcgga tgccgcgcgg ccggttcgcc gaagatccgg tcgatcacga cgccggcaat
     2521 ggcgagcatg gccgtgacat agaaggagag cagcagcatg tcagcctttg agtacgagcg
     2581 gcaggccggc gaccatcatg gtcacgtgcg tgctcgcggc ggcgacgcgc tggttgagcc
     2641 ggccgagttc atcgacgtaa aggcgcgtca tcgatcccat cggcaccacg cccagaccaa
     2701 tctcgttgct caccacgatc accttgccgc gcacggttga taatgcagcc tcgaagtcgc
     2761 tgaacgcttg ccgcggcggc ccgtcgggcg tgccgccgtc cgctgggaac agcaggttgg
     2821 cgagccataa cgtcaggcag tcgatcagca ccacatgacc ttcgcgatcc agttcgcgca
     2881 acacgccgcc cagatcgagc ggcgcttcga ccagcgccca atgcgccggc cggcgttcgc
     2941 ggtgctgctg cacgcgcgcg gcgaattcgg catcgccaat acgcgccgtc gccacgtagg
     3001 taacgggcaa gccgctgtcg gcggccaacc gttcggcatg cgcgctcttg cccgagcgcg
     3061 cgccgccgag aatgaaggtg aggtcgggtg aagtcatcgc aatattgtac cggcgggcgc
     3121 taccatagcg gcttcccaga gaaccgatgg aaccgatggc gacgcaaacc cccatgcaat
     3181 ttgcaccgcg cggcacgctg atgatccagg gcacgacctc cgacgccggc aagagcacgc
     3241 tggtcgcggg cctgtgccgg ctcgcgcggc gcggcggcac gcgggtagcg ccgttcaagc
     3301 cgcagaacat ggcgcttaac agcgcggtga ccgtggacgg cggggagatt ggccgcgctc
     3361 aggcgctgca ggcgatcgcg gcgggcgtgg acgccgaaat cgacttcaat cccgtgctgc
     3421 tcaagccgac cagcgatcgc ggcgcgcagg tgatcatcca cggccacgcg cacgccaatc
     3481 tggatgcccg ggcgtatcac gagtacaaaa ccgttgcctt cgacgccgtt ctcgcctcgt
     3541 acgcccggct tcagcggcgt tttgacgcga tcatcgtgga aggggcggga agcccggccg
     3601 aggtgaatct gcgagaccgg gatattgcca acatggggtt tgccgagcgt gtggattgcc
     3661 cggtggtgct ggtggcggat atcgaccgcg gtggcgtgtt cgcccatttg ctcgggacgc
     3721 ttgcgtgttt atcggagact gaaagggcgc ggattcgtgg cttcgtgatc aaccgttttc
     3781 gaggcgatat cagtctgttg cagcccggtc tcgactggct ggaagcgcaa accggcaagc
     3841 cggtgctggg cgtggtgccc tatctgcacg gcctgacgct ggatgccgag gacatgttgc
     3901 cgagccagct gcacacgcgt ggcgatgccg gcggcgaggt tttgaaggtg atcgtgccgg
     3961 cgttgccgcg catcagcaat cacacggatt tcgatgcatt gcgcgcgcat gcacaggtcg
     4021 atttctccta tgtgcgtgca ggaacggcgc cgccgccagc cgatctgatc gtgctgccgg
     4081 ggtcgaaaag tgtgcagcgc gacctggcct ggatgcgcga aaacggctgg gatcgcgcga
     4141 tcgagcggca tctgcgatac ggcggcaagg tgctgggcgt ttgcggcggc ctgcagatgc
     4201 tgggccgcac ggtggatgac ccgcagggat tcgaagggcc ggctggctgc gtgaatggcc
     4261 tgggcctgct cgatctcgac accacgctga cggcggacaa gctgctcgac aacgtgaccg
     4321 gccggctgac cttgggcgcc ggccccaacc cggcgcccgt gcggggctac gagatccaca
     4381 tgggccgcac gacaggcgca gcgctcgccc ggccggccgt gatgcttgac ggctggcgtc
     4441 cagacggtgc gatttccgcg gacaaccaga tcgccgcaac ctatctgcac ggcatcttcg
     4501 acaccccgga agcctgtgcg gcgctgctcg aatgggccgg tctgagcaat gccgaagcgc
     4561 tcgattaccc ggcgctgcgg gaggcttcgc ttgaccgtct cgccgatacg ctcggtcaag
     4621 cgctggatct tgacgctatc ggcgcgatgt tcgcctgaag gaagcgggcg actgcgtaag
     4681 ccctgcttca atacaggatc atctgcgtgc atcgaaacaa cgcgatggtt ttgccctcgc
     4741 cattcgtcac gatcgcgtcc cacacatgcg tgctgcggcc caggtgaacc gctgtcgcct
     4801 tcgccgcgat gaccccgtcg cgcgccgtac ccacgtgatt gctcttcagc tcgacggttg
     4861 taaaattctt cgcgccctcg ggcagatggg cgatgcaggc gtagccgcaa caggtgtcgg
     4921 cgaggccgat cactgtcgcc gcatgcagga agccgttcgg tgcaagcaat tcgtggcgga
     4981 tggtcagttt gccggtgagc gtgccgcttt ccagcgatac cacttccacg ccgagcagat
     5041 ccggcaggca gccgcgctgg cgttcgcgca gagagtcaag ggtgatgtcg gggcgcagtg
     5101 tggccatgtt gcggagtcct tgaagttgag cggcgttgtt gacgcgcatg gattgaaata
     5161 ggcaaatccg atagtatcaa cggctcgaac atttcactta atgcagcgct tacagcacgc
     5221 cgggattgtc gggcgtcggg cgcggccgac aacgcgtgga ttgaacctat gacggtgatc
     5281 gtggtggcga atccgaaggg cggcgtgggc aagagcacgc tgtccacgaa tctggctgga
     5341 tattttgcgg caaacggcga atgggtcgcg ctcgcggacc tggacaagca gcagtcagcc
     5401 cacggctggc tgggcttgcg cccggacacg ctgccgaaaa tagaagagtg gaggacaaac
     5461 gtcgacgcgc cgtctaagcc gcctagaggt ctggagaagg cggttgtcga cacgcctgcc
     5521 gggattcacg gcacccgtct cgcgctggcg ctggaactgg cggacaaggt gatcgtgccg
     5581 ttacagccgt ccatgttcga tattctcgct acgcaggaat tcctcgaacg gctgcagaaa
     5641 gagaaggcga tacgcaaggg tacgatcgag gtcggcgtgg tgggtatgcg ggtggacgcc
     5701 cggacgaaat cagcggatca actgcatcgg ttcgtggaag ggcttgacct gccggtgctg
     5761 ggttttatcc gcgatacgca gaactacgtg caggtcgcgg cgcacgggct gaccatctgg
     5821 gacgtcgcca aaagccgggt ggaaaaggat atcgagcagt ggcagccgat tattgaatgg
     5881 gtgtcgaaga agtagaagtc ggcatcctcc gccataaaaa acgccggtcg gcttcaattc
     5941 catgaaagcc aaccggcgtt tttgcgtctt gcactggcag aacccgattc aggtccagtt
     6001 ctgcgtgggc acgtgatcgc ggtttccctt gatacggttc ttttcgtcaa cgaacacgag
     6061 atccggcttc cagcccgcct tcagttccgc ctcatcgacc agcgcgaacg cggcaatgat
     6121 caccaggtcg cccaattgcg cccgtcgagc tgccgatcca ttcagcgaaa tcatcccgct
     6181 gccgcgctcg cccttgatgg cgtacgtcgt caggcgctcg ccgttgttca cgttccagat
     6241 gtcgatctgc tcgttttcaa ccagattcgc ggcttccagc aaatcctcgt cgatggcgca
     6301 cgagccttcg tagtgcaatt cgcaatgcgt caccgtggcg cggtgaatct tcgatttcaa
     6361 aagcgttcgc tgcatgcgtt tctctctcaa acttcgttca aatttccagg ttatcgatca
     6421 agcgcgtcgt gcccagcttc gcggcggcca gcacgacgag cggggtcgcg gtatcggccg
     6481 ggccgggcgg cagcaggtcc acgcgccttc tcaccgcgat gtaatcaggc tgccagccac
     6541 gctgcgcaag ggattccacg gcttcttttt ctatcgatgc aaagtcgcga ttgcccgcca
     6601 gcactgcgtc gcgtacgcga ttcagcgctt tcgcgaggat tggcgcctcg gcgcgctcgg
     6661 ccgcttgcag gaatcgattg cgcgagctga gcgccaggcc gtcggcgtcg cgcacggttt
     6721 cagcagcgat gatttccgtg ggcaatgcaa actggtggca catctgccgc acgatcatca
     6781 gctgttgata atccttcttg ccgaagacgg ccacgcacgg ctgaacgcag gacatcagct
     6841 tcatcacgac cgtacacacg ccctggaaga aacctgggcg gaattcgcct tccaggatgt
     6901 cgcccagatt ggttggcgga tgaacccggt attgctgcgg ctccggataa agatcggctt
     6961 cggttggcgc gaacagcacg tacacgttct cggactgcag cttttcgatg tcgtcctgta
     7021 gcgtgcgcgg gtatttatcg aaatcttcgt tcggtccgaa ctgcagccgg ttcacgaaaa
     7081 tgctggcaac caccggatcg ccgtgctggc gcgcgagccg catgagcgac aagtggcctt
     7141 cgtgcaggtt gcccatggtc gggacaaagg cggttcggtt ctggccgcgc aactggtcgc
     7201 gcagttcatg gatcgagctg atgactttca tctagcgggt gtctcctggc gggcagttgc
     7261 gaaaacactg ctccaagcgg aaagaaaggg gaagggcgct gcgctgctta tgcattgtgg
     7321 aaggccgcgc cacggaagct taagccggcg aatacgcgag tctcacgtag atgggcgcat
     7381 acggctcggc ctgagtgatt tcaatcagcg cttcacgcga cagttccagc atcgcgatga
     7441 aattcacgac cacaacggga acaccacgcg acgcgtcgaa gagatcgccg aattccatga
     7501 agcgggcatt ctgcaggcgc cgcaggattg tgctcatgtg ctcgcgcacc gacaattcct
     7561 cgcgcgagat cttgtgatgc tcgaccagcc gggcgcgctt gatgacgtcg gcccaggcgg
     7621 cgcgcaggtc gtttgcatcg acgtcgggga agcgttgtgc ggagctctgc tcgacgtaca
     7681 cctctgcccg caggaagtcg cggccgagtt gcggcaagtg gtccaggcgc tgagccgcga
     7741 gcttcatctg ctcgtattcc agcaagcgtc gcacgagttc ggcgcgcgga tcttccgctt
     7801 cctcacctgt atctgccttt ttcaccggca ggagcatgcg cgacttgatc tcgatcagca
     7861 tggctgccat caacaggtat tccgccgcga gttccaggtt ggattcgcga atctggtcga
     7921 cgtagcccag gtactggcgt gtcacgtcgg ccatcgggat gtcgaggacg ttgaaattct
     7981 gcttgcggat caggtacagc agcaagtcga gcggaccttc gaacgcttcg aggaaaatct
     8041 cgagcgcgtc cggtgggata tagaggtcgg tcggcagccg ccagagcggt tcgccgtaca
     8101 gatgagcaaa cgccacgccg tcaatggtgt tcggcgtgga gtcggtcgca ggcgcggcta
     8161 gcgccgcggc gcgatcatcc ttgcccgaat gcccggggtg tcccgaacgc tccggagctt
     8221 gcggcgcctc gcgggcggca ctcacgttca gaagttctgg taatagacgt agggcgtttg
     8281 ttcgactcgc gatgtcttga tttccgagcg ctcgtcgagg tcgataggct gcttgtccca
     8341 cagcagcgca cggccctggc gctgctctgc ttcgagcgac ggcttctgct ctttcatctg
     8401 attgatgaac tgggtgatgt ccgacaggta catgattcga cttccgttgg ctcagagcga
     8461 ttcgaaaacg ggcgcggcgg aggttccagg cgaatagccg ccgtgaattg ccgccgtgat
     8521 tcatcacgat tttaccgcaa gcatcaggca gcggagcggt taacacgtct caagcaggtc
     8581 cgaagcacct tccaagcacc tcccaagccc gcccgaaggt cgcggacaag cgggtacatc
     8641 ggccattcgg tctaccggag ccgccgtgca ggcaacgata tgcaatcgta aaacgatgtc
     8701 aattattgat gcaggcacgg aacaagctct gcagggagcc gtccaaggcg cacctctgcc
     8761 attttcagaa gcggtggggt taagcggggc ggcgatcggg ttgaatgttg tccgatcggc
     8821 caagctgtcg gcttttggga cagccggccg cgccagtgtg gtgaaatagc gcctgctgga
     8881 ctggcgctcg gcgtgtgcac aacacgttgc cagcatcgag ccgtcaccga acgccggctg
     8941 tcccgtgtca cccacgcagt cccattaatt caaccccgga ggtcacgttt ggcggggcgc
     9001 ctgtttgatc cgctgcgctt caagcgctgg caagccgatg accggcggct gaagccgccg
     9061 gtcatcgcgt cgtcgaaggc gccttcccgt gcgtggtggt tgtcatccgt gcgccgtgtc
     9121 gtctggtcgc tccacgcgct ggcgttgttt gctgtcgccc agcccgcgca cgcgaaatac
     9181 gacgtcgata tcgacgcgcc gcgcagcgtc cggtcgctgc tgaaagacca tcttgatttg
     9241 tcgcgtttca agatgcgtga cgacatcagc gacgagcaat tcgacttcct ggtcacggcc
     9301 acaccgcagg aggtccgcga tctggtcgcg accgacggtt atttcacgcc ggtcgtgcga
     9361 accgacgtga aacatgacgg cgataaaaaa tccgtcacta tcagcgtcga tccgggtccg
     9421 cagaccaagg tcgcctccgt ctcgctgacg ttcaagggtg caatcacgac cgaagacccg
     9481 gcgcagcaaa acactgcgcg tctcgcgttt tcagtcaagg aaggtgatcc gttctcgcaa
     9541 ggcgcgtggg acgacgcgaa gaacgccgcg ctcaaagcgc tgcaggcacg ccggtatctc
     9601 ggcgcgaaaa tctctcaatc caaggcacgc gtgaatccgc gcacgcacat agcggatttg
     9661 tcggtgacat tcgatagcgg cccgaccttc accttcgggc agctcgacat cagcggcgtc
     9721 cggcgttacc cggaaaagat cattcacaat gtgaaccgaa tcaatcccgg cgacacctac
     9781 gacgtcgcgc gcgtgagcga gttgcagcgg cagttgcaga acacgccgta ctacgcgtcg
     9841 gtggctatcg acgcggacaa cgacgtcaac aagccgaatg aaacaccgat gcacctgaag
     9901 gtcagcgagt atccgtacaa cagcgtgcgc tacggcgtgg gttacgcgac agatacgggc
     9961 ccgcacgtcc agggcgctta cagttacctc gataccttcg gcaaggctta tccgtttacc
    10021 gtctccggtc gcctggacca gacccagcaa tacggccagg tgcagctcgc gatgccgccg
    10081 gacggccgca cgtgggtgaa cagcgtgctg gcttcgtaca cgaacacgaa cgtatccgac
    10141 acgcgcatct acagcgcgcg cgtgggcgtg cagcgcatgc gctcgctgca gaacatcgac
    10201 accacgtttg ctctgctgtt atatgccgac cggctgacgc agaatctcgg gccaccaacc
    10261 tccagccgtg cgctcgtgcc gtcgtggcaa tgggtgcgcc gtaatgtgga cgatccgctg
    10321 tttccgcgct cgggcaacct gatccgggcg gaagcggacg tcgcggtgaa gggcatcctc
    10381 accgatcaaa cctttggccg cgtttatacg aacctgctgc aatacctgcc gctcggcaag
    10441 cgcgacctgt tcgtgtttcg cgcggagttc gggggggtgt tcacggcggg cagctcgaac
    10501 ggtgttcccg cgtcgctgct gttccgtgcg ggcggcgcga attcggtgcg cggttacggt
    10561 tatcagagca ttggtaataa cgtcgatggc tccgtcttgc cgaccaagta tctgatgacc
    10621 ggcagctcgg agtaccagca ctggttttcg cacgattggg gcggcgcggt gttcttcgac
    10681 ataggcaccg ccaccgatac ctggaaagag cgcgtgttcg agccgggcac gggcgtgggc
    10741 gtgcgctggc gcagtccggt cgggccggta aacgtggata ttgcctatgg cttcaagaac
    10801 cgggacatcc gcccgtacct gacgctcgga atcgcctttt gatgaatttg cctggattat
    10861 ttcgattgtt ccttcaagca tgagcgacca gaacggccaa tcccccgcag accagccgga
    10921 cccttccgag gaccggggga acggcacgtc tgaaaacgga acgcctgttc cgaagccgcg
    10981 gcgcggcggc tgggggcgcc tggccaaatg gctgggcggt atcgtgctgg tgctagtgct
    11041 gtgcttcgcg ctcggtgtcg cgatgatcct gtatgcggtg aacagcgagc gcgggacgcg
    11101 ctacgtgtgg caggcggcga cgtcgctgct taacgcccgc ttgagcggca cactcgacgg
    11161 cggcgcaata gcaacgggcc tgcgcttgcg caatgtgcat tggaagagtc tggacggcaa
    11221 cggaacggat attgccgtag acagcgtttc cggcaaatgg gaactgacgc gcgcgccgtt
    11281 acgtttcacc atcggttatt tgcatgtcgg tacggtcgat gcacgcattg caccctcccc
    11341 gaaagacacc agcaagaccg agttgccaac ggatttacgc ttgccaatcc agcttgccat
    11401 cagcgacgtc acgctcgaca agctgcgcct ccacgaaggc gcgacaacca ccgaattctc
    11461 gcggctcgca ctcgacggac atagcgatgg ccgccatcac gaggcgcgca tccagcgtct
    11521 cgatacgccg ttcggcgcca tcacggccgc attgaaactc gacggcggcg cgcgcccatt
    11581 tccgctgact ggcgatgccg gttattcggg cgaggtcagc ggcgaggccg tgcaggtggc
    11641 cgcgcatttg acgggttcgc tggaaaatct cgccgccgat atcaacgcca gcggtttgaa
    11701 gctcaagggc caggcgcacg tcgaggctgc gccgttcggc gacgtgccgc tcaagcgcgc
    11761 gctagtgacc atcgatcacg tcaatccgca ggcgttcagc ccgagcgcgc cgttcgcgga
    11821 tctcgcgctg cgggcagaac ttacccccga tccggctacg ccaaacgcgc tggtggtgac
    11881 cgggcctata tcgatcgtga acgcgaagcc cggttcaatc gataagcaac tgcttccctt
    11941 aatcgatgcc cacgcgaacg tgaagctcga tgcatcggcg caaactatct ccgacctgaa
    12001 cctacggctg ttgaaggacg ccatcgtcac cggcggcgga acgctcaagg gcgggacagg
    12061 ccagttcgac ctgaaggtcg cgaaacttga tctgaacgcg atcgaaccga ccatccgtcc
    12121 gacgcagttt gccggtccag tcggcattca tctcgatggt gacaagcaga ccatcacgct
    12181 ggatctgaac gacccgaaag ccgcgttgcg cgcgcaaggc aaggtcacgc tcgacgcgaa
    12241 acaaactgcg tttgacggcg tgaaactgag cgtaggaaag ggccgtatag agttgagcgg
    12301 cgcgctccag aaagacattc attcgagcta cgacctgaag gcgaaggtca tcgatttcaa
    12361 tccgctgctg ctgacggacg aacttgtcgc gaagcctccg ccaaagggat ccaaagcgaa
    12421 gccggtcgcg aagtcccggc ccatcgaagc acacgtgaac ggcacgctgt cggctgcggg
    12481 cgtgctcgcg cccacgctca ccaccaaggc ccagttcaag ctcggcgaca gcgtctacga
    12541 caacttgccg ctcaccggct cgggcacgat ccaggtggtg ggaacgcgca tcctgccgag
    12601 cacggcaacg ctatcgatcg cgggcaacaa cgtcgatcta aacggcagtt tcggcgcgcc
    12661 gagcgatcgc ttgcgttttc gtgtcgatgc accgcaactc gagcgcctgg gcttcggact
    12721 ggcaggactc gtcaaggccg acggcgatat caccggttct ttcgcgcatc cgaatctggc
    12781 ggctaactat caggtcgaca gcgtggtgtt cggtgcgaat cgtgttggcc atgcggaagg
    12841 tcgggcggaa gcccgcgacg gcgcgaacgg cgcgctggtg ttcacccttc aggcccgtga
    12901 tgtcagcacg gacggcgtcg atctcgcgag tctggacgcg aacctcaacg gcacgcgcgc
    12961 caaccatacg ctcaacgcga ccgctgtcgg caaggtgcgc ggcaacgcgg tcaacctggc
    13021 gctggccgca aacggccgcc tgaccgatgc ccgtgacggt gcccattggg acggcacggt
    13081 gacgcgcttg tcgaacaagg gcacgccgaa cgttaatctc gacgcgccgc tcaccgtgag
    13141 ctacgcgccg aatcatgtag cgctgggcgc gacgcgttta tcggcggaag gcgcggtgct
    13201 cgacctgaaa tcgttcgcgc tcgatggcgg gcgcatcaat tcggctggcc agttgacgaa
    13261 cctctcggtg gcgcgactgc tcgaaatccg tcaggaactg gagggcggac cgccgcccgc
    13321 caggaccgac ctggttttcg atggcgactg gaatttcgcg ctcggcgcca ccgcgacggg
    13381 ctacgtgcaa ctgaagcggc gttcgggcga tgtctctatc gatgcagccc gcggcgtcgc
    13441 ggcgctcggc atcacggaca tcaccgcccg cgcggacttc accggcggca atcggcttaa
    13501 cgcgacggtg cacgcgcagg ccacgcgaat cggcgtgatc gatgccaacg tccacacgaa
    13561 cctggtggcg cgcgacggcg tgctcacggt cgccgacgaa gcgccgctga gcggagcgat
    13621 tgaagcgaac gttcccacgc tgcgcacgac cggcgggctg ttcggcccgg cttatctgct
    13681 tgatggccgc cttgggctca agctgactat cgcggggatc gttgcgaaac cgacgttgtc
    13741 cggcatgctc accggtgacg cgttgtcagt cacggtcatc gatcagggcg tgcagttgaa
    13801 ggacggcgtg atccggattg ctttgtcgga gaatcttgtc gatcttcaac aggtcgagtt
    13861 tcacggcgcc agcggcacgt tgcgtgcgac cggcaaggtg cggctggatc agcagcagcc
    13921 ggacctgacg gccagcatca ttgctgacaa gctggaactg tttgcgtcgc ctgaccggca
    13981 gttgcagttg tcgggcagtg cttccgtggc gaacgcgggt ctgcagggcg ggatggcgat
    14041 tgacggcaag ttcaccatcg atcacgcgct cttcgatttg cccgagcaat cggcgccgtc
    14101 gctcggcgac gacgtcgtga tcgtgcgtcc cgacggtacg gtaaagggcg aggatcagaa
    14161 ggtggtcgca accggagaca agcccgtggg cgcgttcgcg ccgcgcgcga atatcgatat
    14221 caatctcggc cagaaattcc gcttccgtgg agcgggcgcg gaccttgggc tagccggcac
    14281 cgtcacggca atgagcgcgc cgaacatgcc gctgcgggca gtcggcaacg tgcgcgtgac
    14341 gccgggttcc acctatacgg cgtttgggcg gaggctggga atcgaaaacg gctttttcac
    14401 attcaacgga ccggtcgcga atcccggcat caatattctg gcgatgcggc gcaatcagga
    14461 gatcgaggcc ggcgtgcagg tgacaggcac ggtgcagtcg ccggttgcca agctgatttc
    14521 agagcccaac gtgccggata acgagaagtt gtcgtggctg ctgttcgggc atggcacgga
    14581 tcaaggcaac aacgtcggcc agcagagcgc gatgaccaat gcgctcgcat tgctcggaag
    14641 ttcgcagggc aagcggattg cgcagacgtt cggcctggat gagttttcta tcgggacgag
    14701 tgaggtgggg ctgaccgatc cgcaagtcgt gatgttgtcg aaggcgatca atgaacggct
    14761 cgtgctcggt tatgagcagg gtctgcaatc ggcgagcaat gcaatcaagg cgacgctgag
    14821 cctgtcgcgg ttctggtcga tatcggcgta cggcggcacg ttccagggca tggatctgct
    14881 ttatacccgg cgattcgatt cgtggtcgcg aaggaacggg ccgggcgact cgaagtaggt
    14941 tgcgaagtgc acgaacgtta aaaggccgta ttcaacgtga gttgcatcag ttgaatacgg
    15001 cctttgtttg gtcttgcgtt ttgttgcgcc cggtactttc agttcacgct ttacttcacg
    15061 cgcatgcccg gcttcgcgcc gctgtgcggt tcaagaatgt acaagcccgg ttcggctttc
    15121 tcgtcgtcgg cggaagcggc cagcaccatg ccttccgaca gcccgaattt catcttgcgc
    15181 ggggcgaggt ttgcgaccat gaccgtgaac ttcccgatca gatcctgtgg ctgatatgcc
    15241 gacttgatac cggagaacac gttgcgggta ttttcctcgc caatatccag cgtcagttgc
    15301 aacagcttgt ccgagccttc gaccgccgtg caattcacga tcttcgcgat tcgcagatcg
    15361 accttggcga agtcatcgat ggaaatcacg ccttccgctt ccggctcttt cactttcgct
    15421 gccttcttcg acgaggaact ggaagcagcc gcaggcgcgg cggcaaccgc ttgccccggc
    15481 gcaaccgatt cccggttcgc cgcgatcagc gcttcaatct gcttcggatc gacacgcgtc
    15541 atcaagtgct tgtacgcgtt gatcggctgc gaggaactga gcggcgcgga gacatccgcc
    15601 cagacaagcg gcgcgatgcc caggaatcct tccaccgatt ccgccactac cggcaacacc
    15661 ggcttcaacg ccagcgacag caagcggaat gcttccaggc tcacgctgca ggtttcgtgc
    15721 agcgcggcgg cgttggcttc gtccttggcc tgatcccagg gcttcgcggt atcgacaaac
    15781 gcgttgacgg catcggcgag atccatggtc gtgcgcaacg cccgcccgta ttcgcgcgct
    15841 tcgtagagcg ccgcgatctg cggcacggcg ccgcgcaatt gcagcagcag cggatgattc
    15901 attacgctgt ccagcacacg gccttcgaaa cgcttgatca ggaaccctgc cgcccggctc
    15961 gcgatattca cgtacttgcc gaccagatcg ctgttcacac gtgcctgaaa gtcgccaaga
    16021 ttcaggtcga tgtcttccat cgtgtggttc agcttcgcgg cgaagtagta gcgcagccac
    16081 tcggggttga tgcccgtatc gatcacgctt ttcgccgtaa tgaacgtgcc gcgcgacttg
    16141 gacatcttcg tgccgtcgac ggtaagaaag ccgtgagcaa acacgttcgt tggcgtccga
    16201 tggccggaaa attccagcat cgcgggccag aacagcgtgt gaaaatacag gatgtccttg
    16261 ccgatgaaat ggtgcagctc ggcgcgcgag tcggcgccga aaaagtcgtc gaagcgcagg
    16321 ccggtgcggg cgcagagctg cgtgaagctc gccagatagc cgatcggcgc atcgaaccag
    16381 acgtagaaat acttgccggg cgcatccgga atctcgaagc cgaaataggg cgcgtcgcgc
    16441 gagatgtccc agtcctgcag gccggccttg agccattcgt cgagcttggc tcgcacggcg
    16501 tcatccggag cggcg
//
LOCUS       DATASH010000865         2221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1137535,
            whole genome shotgun sequence.
ACCESSION   DATASH010000865 DATASH010000000
VERSION     DATASH010000865.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1137535"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1509)
                     /locus_tag="VHV99_23155"
     CDS             complement(<1..1509)
                     /locus_tag="VHV99_23155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638321.1"
                     /translation="MTRGLNYSGRFRILTPRSKTDRSPTGLRASSGLRRGRFSAKTTG
                     PIDWDEVRPAIREEMLPVILASILLAFMALMLSFSRGYVLLYGDAVAHLGIARRILDT
                     RNPGLVQLGGVWLPLPHLLMLPFVQRMEWWQSGLAGAWPSIGCYIAGVAGFYRLCRRM
                     LVPRWALTATAFYAVNPNLLYLATTAMTEPLFLALIIWMTLLTVECIDEIRADRQSGV
                     ARRLIGLGLLIFAAVFTRYDGWVLGAAVWCVVTGQLWRVRGMWRRVVPSFAVFTLLAL
                     SGPMLWFWYNQHFFHDPLDFMRGPYSAPAIEKKTSPPGSRHYRGWHNPAWALLFYTRT
                     AQVVAAAWETGFALMAAAVAGLWIAWRRRIKPASLLLWVPLAFYVYSVAYGSVPIFIP
                     QLWPHSYYNARYGMELLPALALFACVAAAALELKLKSAAAARVDGIAPEGRRPRWKLL
                     YPTALTLAFVNALAMMYGTTVVQRVVSLVRHRPSHVMAIYDPPLVLKEAIVNA"
     gene            complement(1454..2221)
                     /locus_tag="VHV99_23160"
     CDS             complement(1454..2221)
                     /locus_tag="VHV99_23160"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX3638322.1"
                     /translation="MFKARGIDTRGIERAPGLSFHWTGAYAGTLSEAQTLGTDLNVFE
                     KFEPKIPVEYLDTEYLFLANIDPVLQARVRSQMSNVRMVCGDTMNYWIKDHTENLLKV
                     LKELDVLLINDGEARMLSGEQNPVRAAEAVLAMGPKSLVVKHGEYGATAFFGERSFFG
                     ASSVPRPFRAPALPLAEVVDPTGAGDSFAGGFYGYLASQPELTPAVFRKAMFYGGVMG
                     SFAVERFGTERLQAVSREEIDARFELFREISHLDTAV"
BASE COUNT          500 a          786 c          579 g          356 t
ORIGIN      
        1 tgcgttgacg atcgcctctt tcagcaccag aggcggatcg tagattgcca tcacgtgcga
       61 cggccgatgc cgcaccaggc ttaccactcg ctgcactacg gttgtcccgt acatcatcgc
      121 cagcgcattt acaaacgcca gcgtcagagc ggtcggatac aacagcttcc accgtggccg
      181 acgaccttct ggagctattc cgtcgaccct agcagcagcc gccgatttca atttcagttc
      241 caacgcagcg gccgcaacgc acgcaaacag cgccagcgcc ggcaacaact ccatcccata
      301 tcgcgcgttg tagtacgagt gtggccacag ctgcgggata aagatcggca ccgacccata
      361 cgcaacggaa tagacataga acgccagcgg aacccacagc agcagcgacg ccggctttat
      421 ccgccgccgc caggcgatcc acaatccggc caccgcggcc gccatcagcg cgaagcctgt
      481 ctcccacgcc gccgccacta cctgcgccgt tcgcgtgtag aacagcagcg cccacgccgg
      541 attgtgccag ccgcgataat gcctcgaccc gggcggcgag gtcttcttct caatcgccgg
      601 cgccgaataa ggtccgcgca tgaaatccag cgggtcatgg aaaaaatgct ggttgtacca
      661 gaaccacagc atcggccccg agagggcaag cagtgtgaac acggcgaacg acggcaccac
      721 gcgccgccac attccgcgca cccgccaaag ctgccccgtc acgacgcacc aaaccgccgc
      781 acccagcacc caaccgtcat agcgcgtgaa aaccgccgcg aagatcagca agcccaagcc
      841 aatcaatcgc cgcgcaacgc ctgactgccg gtccgcacga atctcgtcga tacactccac
      901 ggttagcagc gtcatccaga tgatcaacgc cagaaacagc ggctcggtca tcgccgtcgt
      961 cgcgaggtac aacaggttcg ggttcaccgc gtagaacgcc gtggccgtca gcgcccatcg
     1021 cggcaccagc atgcgccggc acaggcgata aaagccggct acgccagcga tgtaacagcc
     1081 aatcgacggc catgcgcccg ccaacccgct ctgccaccat tccattctct ggacgaacgg
     1141 aagcatcagc agatgcggca gcggcagcca tacaccgccc agttgtacca gcccggggtt
     1201 gcgcgtatcg agaatccgtc gcgcgattcc caggtgtgcc accgcgtcgc catacagcag
     1261 cacgtatccg cgtgagaagc tgagcatcag cgccataaat gccagcagaa tcgacgccag
     1321 aatcaccggc agcatctcct ctcggatcgc cggcctcacc tcgtcccagt caatcggacc
     1381 agtcgtcttc gcactgaatc gcccacgcct cagtcctgat gatgcgcgca atccagtggg
     1441 actgcgatcc gtcttagacc gcggtgtcaa gatgcgaaat ctcccggaat aattcaaacc
     1501 gcgcgtcaat ctcctcacgc gaaactgcct gcagccgctc tgtgccaaag cgctccacgg
     1561 cgaacgagcc catcacgccg ccgtaaaaca tcgccttgcg aaataccgcg ggtgtaagct
     1621 ccggttgcga cgccagatag ccgtagaagc cgcccgcaaa cgaatcgcct gccccggtcg
     1681 gatcgacaac ttccgccagc ggcagcgccg gagctcgaaa cggacgcgga actgaagacg
     1741 caccaaagaa tgaacgctcg ccaaagaagg ccgtcgcgcc atattcgcca tgcttgacca
     1801 ccagcgactt tggccccatg gccagtacgg cctccgctgc gcgcaccgga ttctgctcgc
     1861 cggatagcat acgcgcttca ccgtcgttga tcagcagcac gtccagctct ttaagcacct
     1921 tcagcaggtt ctcagtgtga tccttgatcc agtagttcat cgtatcgccg cacaccatgc
     1981 gcacgttcga catctggctg cgcactcgcg cctgcagcac tggatcgatg ttcgcgagaa
     2041 aaagatactc cgtgtccagg tactccactg gaatcttcgg ctcgaacttc tcgaacacat
     2101 tcagatcggt gcccagagtc tgcgcttcgc tcagggtccc cgcatacgcg ccggtccagt
     2161 ggaaactcaa gccgggtgcg cgctcgatgc cacgcgtatc aataccacgc gccttaaaca
     2221 c
//
LOCUS       DATASH010000866         1816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_939533,
            whole genome shotgun sequence.
ACCESSION   DATASH010000866 DATASH010000000
VERSION     DATASH010000866.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939533"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..929
                     /locus_tag="VHV99_23165"
     CDS             222..929
                     /locus_tag="VHV99_23165"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX3638323.1"
                     /translation="MFWALLALPAFLMPRAAVLKVASWWAKSAIFLMRVICNIKVEFR
                     GVEKIPKGPLIVASKHQSMFETIALLGFFEAPFFVVKRQLNFIPLFGQYLVKTGMVAI
                     NRSEGRRALLSVMRRAAEEVKHGRQFVIFPEGTRTAPGAPPQYKGGVGMIYSDCGVPC
                     LPVALNSGLFWPRRTFMRYPGTLVLEFLDPIPPGLKRDEFLARVERTIENATDRIVAA
                     GREEQRRLFGGIPGKGE"
     gene            complement(959..1804)
                     /locus_tag="VHV99_23170"
     CDS             complement(959..1804)
                     /locus_tag="VHV99_23170"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX3638324.1"
                     /translation="MARIVGARMGELLGQPVIVENVTGAAGIIGVERVINAKPDGYTV
                     LLGTVGTHAYNQSIYRKRRYDAVGDFTPITLFSDQPMVLEGRKDLPANNLAEFIALVK
                     QNGTKMQYGSAGVGSTTHLACALLNARIGVNVTHVPYRGGGPAANDLIGGHIDYMCGN
                     IGTVVGRAAAKQSKPLALLSRERTPLMPELATAHEQGITDYDVVTWTAFFLPKGAPKD
                     IVAKLNEATHAAMDTPEIKTRFNAIGVVGVAPERRGPEYLAKYVVEEIARWEGPIKAA
                     GLQVD"
BASE COUNT          316 a          554 c          574 g          372 t
ORIGIN      
        1 ggtttggcgt cgatccgacc ctgttcgagc cgtccgacca gccgaccggt tcgctgcagg
       61 cgcaccgccc cgccaccgca caagctaact agggcatgat ccggaaaagt gtgaaacggt
      121 tttccgaaaa gatcatgcgc aaagaataaa gtcctggaat gtcgatgacc ctgatcttcc
      181 tgcgttcgct ggtcttcaac atcctgttct acgccgcctt cgtgttctgg gcgctgcttg
      241 cgctgccggc gttcctgatg ccgcgcgcgg cggtgctgaa ggtcgcgagc tggtgggcga
      301 agagcgcaat cttcctgatg cgcgtgatct gcaacatcaa ggtcgagttt cgcggggtcg
      361 agaagatccc caagggaccc ttgatcgtcg cttccaagca tcagtcgatg tttgagacca
      421 tcgcgctgct cggcttcttc gaggcgccat tcttcgtggt caagcggcag ctgaatttca
      481 ttccgctgtt cggccagtat ctggtcaaga ctggaatggt cgcgatcaat cgcagtgaag
      541 gcaggcgagc gctgctttcg gtgatgcggc gtgccgcgga agaggtgaag cacggccggc
      601 aattcgtgat ctttccggag ggcacgcgca cggcgcccgg cgcgccgccg caatacaagg
      661 gcggtgtcgg catgatctac agcgactgcg gcgtgccctg cctgccggtc gcgctcaatt
      721 ccggtctgtt ctggccgcgc cgcactttca tgcgctatcc cggcacgctg gtgctcgaat
      781 tcctcgatcc gatcccgccg gggctgaagc gcgacgaatt cctcgcgcgt gtcgaacgca
      841 cgatcgagaa cgcgaccgac cgcatcgtcg cggcggggcg ggaggaacag aggcggttat
      901 ttggggggat accggggaag ggggagtgat tttcagatcg tccggcggcg ggccggcatc
      961 aatccacctg caaacccgca gccttgatcg gaccttccca gcgcgcgatc tcctcgacca
     1021 catatttggc aaggtattcg ggcccgcgcc gctcaggtgc gacgccgact acgccgatgg
     1081 cgttgaaccg tgtcttgatc tcaggcgtgt ccattgcggc atgggttgcc tcgttcagtt
     1141 tggccacaat gtccttcggc gcgcctttcg gcaggaagaa agccgtccag gtcacgacgt
     1201 cgtagtcggt gataccctgc tcgtgcgccg tcgcaagctc cggcatcagc ggcgttctct
     1261 cgcgcgacaa cagcgcgagg ggcttcgatt gcttggcggc ggcacgcccc acaacggtac
     1321 ctatgttgcc gcacatataa tcgatgtggc cgccgatcag gtcgttcgcg gcaggcccgc
     1381 cgccccgata gggaacgtgg gtcacgttga cgccgatcct cgcgttgagc agcgcgcagg
     1441 caagatgcgt agtcgatcca acgccagccg agccgtattg catcttcgtg ccgttctgtt
     1501 tcaccagcgc gataaattcc gcaaggttgt tggcgggcag atctttgcgg ccttcgagca
     1561 ccatcggctg gtcggagaag agcgtgatag gggtgaagtc gcccaccgca tcatagcggc
     1621 gctttcggta tatcgattga ttgtaggcgt gggtgccaac agtgccgagc agcaccgtat
     1681 agccgtccgg cttggcattg atcacgcgtt cgacgccgat gatgccggct gcgccggtca
     1741 cgttttccac gatcaccggc tggccgagca gttcgcccat tcgcgctcca acgatgcggg
     1801 ccatcgtgtc gaaaac
//
LOCUS       DATASH010000867         1998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2521526,
            whole genome shotgun sequence.
ACCESSION   DATASH010000867 DATASH010000000
VERSION     DATASH010000867.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2521526"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..987)
                     /gene="puuE"
                     /locus_tag="VHV99_23175"
     CDS             complement(37..987)
                     /gene="puuE"
                     /locus_tag="VHV99_23175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase PuuE"
                     /protein_id="HEX3638325.1"
                     /translation="MPLDSNYPRDLIGYGRYPVQANWPGRARVAVQFVLNYEEGGENC
                     VLHGDPGSEQFLSEIVGAAAYPARHMSMESIYEYGSRAGVWRILREFEKRGLPLTVFG
                     VGMAIERHPDLGRAFVELGHEIACHGYRWIHYQNVSPEKEAEHMRLSMEAIERITGER
                     PLGWYTGRDSPNTHRLVAEHGGFLYDSDYYGDDLPFWMDVEVTGGAKSPQLIVPYTLD
                     TNDMRFASPQGFNTADHFFTYLRDAFDVLYEEGDEAPKMLSIGMHCRLLGRPGRFRAL
                     QRFLDHIEQHDRVWVTRRVDIARHWREHHPYQQNNRGAAA"
     gene            complement(1220..>1998)
                     /locus_tag="VHV99_23180"
     CDS             complement(1220..>1998)
                     /locus_tag="VHV99_23180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007588261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEX3638326.1"
                     /translation="EVIAVNPTMGPPSIEGYYDEALATPGLLAEVIAGEREGCDGYVI
                     ACFGDPGLYAARELARGPVIGIAEAAMHAASVLAPGFSVVTTLARTCGMAWHLAERYG
                     MKRFCRNVRATDVAVLDLDKPGSAARRVILDECRRALEEDGSDAIVLGCAGMAELCAE
                     IEDALGAPVVEGVTAALKWTEALVALRLSTAKRGDYARPLVKRYGGALEAFSPTDADP
                     NPQPPRDSAASTAESANSSGLLRVNTTAGPVGPASHILPV"
BASE COUNT          371 a          631 c          624 g          372 t
ORIGIN      
        1 tgctgttgag ttggtccaga gtgtattgca tcgccttcat gccgctgccc cgcggttgtt
       61 ttgctggtaa gggtgatgtt cgcgccagtg acgcgcgata tcgacgcgcc gcgtcaccca
      121 cacgcgatcg tgctgttcga tatggtcgag aaaacgttgc agcgcacgga aacgcccagg
      181 acggccaagc aaacggcagt gcatgccgat cgacagcatc ttcggggctt cgtcgccttc
      241 ttcgtagagc acgtcgaacg cgtcgcgcag gtacgtgaag aaatggtccg ctgtgttgaa
      301 gccctgcggg cttgcaaagc gcatatcgtt ggtgtcgagc gtgtacggca cgataagttg
      361 tgggctcttc gcaccgccgg ttacttcgac atccatccag aacggcaggt cgtcgccgta
      421 ataatccgag tcgtacagga agccgccgtg ttcggcgacg agacgatgcg tgttgggact
      481 atcacggccg gtataccagc cgagcgggcg ttcgccggta atgcgctcga tcgcttccat
      541 gctgaggcgc atgtgctccg cctctttttc cggcgacacg ttctggtagt ggatccagcg
      601 atagccgtgg caagcgatct catgaccgag ttcgacgaag gcgcgcccga gatcaggatg
      661 ccgttcgatc gccatgccga cgccgaacac cgtgagcggc aggccgcgct tttcgaattc
      721 acgcaggatg cgccatacgc ctgcgcgcga gccgtattcg taaatcgact ccatgctcat
      781 gtgacgcgcc ggataagccg ccgcgcccac gatttccgac agaaattgct ccgaacccgg
      841 atcgccatgc agcacgcagt tttcaccgcc ttcttcgtag ttcaggacga attgcacagc
      901 gacacgcgct cgacccggcc agttcgcctg caccgggtag cggccgtagc cgatcagatc
      961 gcgtgggtag ttcgagtcga gtggcatggt ttgacgaaag cgtggtgacg gtacgtaaaa
     1021 ttcgcgtgaa actcacgtga catgcacgtg gggtctgttt gcgtatgttt ggatatgact
     1081 cacgccggcg cggggaaaac agccactcaa tagtggcacc agaagcagca gcaaaatagc
     1141 gttcgggcac cagcgccgag tgggatcagt gtagcgaaaa cgcccgggtg cgcccataca
     1201 cccgggcaga tagtgcgtgt cagaccggaa gtatgtgcga agccggtccc accgggcctg
     1261 ccgtggtgtt tacccgcagt aagccgcttg aatttgcgct ttcggcggtg cttgcggccg
     1321 aatcgcgggg cggctgggga ttggggtccg catcggtcgg actgaaggct tccaatgcgc
     1381 cgccgtaacg cttgaccagt ggccgcgcat aatcgccccg cttggccgtg gagagccgca
     1441 gcgcgaccag cgcctcggtc catttgagcg cggctgtcac gccctcgact accggcgcgc
     1501 ccaacgcatc ttcgatctcc gcgcacaatt cggccatgcc ggcgcaaccg agcacgatcg
     1561 cgtccgaccc atcttcctcc agcgcgcgcc ggcattcgtc gaggatgacg cggcgcgccg
     1621 ccgagccggg cttgtccaga tcgagcacgg cgacgtcggt ggcccgcacg ttgcggcaga
     1681 aacgcttcat gccgtagcgc tcggcgaggt gccacgccat gccgcaggtg cgcgccaagg
     1741 tggtcaccac cgagaacccc ggcgccagca cgcttgccgc atgcattgcg gcttcagcga
     1801 taccgatcac cggaccgcgc gccagctcgc gtgccgcgta cagaccgggg tcgccgaagc
     1861 aggcgatcac gtaaccatcg cagccctcac gttcgccggc aatgacctct gccagcaagc
     1921 cgggcgtcgc cagtgcttcg tcgtaatagc cttcaatcga cggcggcccc atcgtcggat
     1981 tcaccgcaat cacttcgg
//
LOCUS       DATASH010000868         4791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1731403,
            whole genome shotgun sequence.
ACCESSION   DATASH010000868 DATASH010000000
VERSION     DATASH010000868.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1731403"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1600
                     /gene="bamA"
                     /locus_tag="VHV99_23185"
     CDS             <1..1600
                     /gene="bamA"
                     /locus_tag="VHV99_23185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006758559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEX3638327.1"
                     /translation="RGYLEFNIDSTQVSISPDKKDMYLTVTLHEGEPYTISSIKLAGN
                     LLDREPELAKLIKIKPGDRFSAEKLQATTKSIVDKLGEYGYAFATVNAQPQIDQEHHK
                     VDLTLQVDPSRRVYVRRINVVGNTRTRDEVVRREMRQLESSWFDSNRLALSKDRINRL
                     GYFTDVDVTTVPVEGTPDQVDVDVKVAEKPTGAITLGAGFSSTDKVVLSAGVSQDNVF
                     GSGTSLAVNVNTAKTYRTLTVTQVDPYFTVDGIKRITDVYYRTYQPLYYSTDSSFKIV
                     TIGGDLKFGIPFSEVDTVYFGAGLEQNQLDIDANTPASYIQYVKDFGRVSNNVPLTVG
                     WSRDARDSALVPSRGYFTQANAEYGTPIGGTQYYKADINAQYYYSFARGFVLGFNFQG
                     GYGNGLGGKPYPIFKNYYAGGIGSVRGYEPSSLGPRDKTTGDPIGGSKMLVGNVELTF
                     PLPGTGYDRTLRVFTFLDAGNVWADASSAATSVGSNGLRYGYGVGLAWISPIGPLKLS
                     LGFPLTKHTGDQYQKFQFQIGTAF"
     gene            1662..2198
                     /locus_tag="VHV99_23190"
     CDS             1662..2198
                     /locus_tag="VHV99_23190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEX3638328.1"
                     /translation="MTLLTGMFSKRVACAFALAMTLGVGMAGAAHAQEARIAAVNSDR
                     ILRESAAAKAAQVKLQAEFASRDKDLADKTQAIKALADELDKKGASMQPEVRAQKQRT
                     LSQMDFDLQRSQREFRDDLERRRNEELAAVLDRANKVIKQIAEQQHYDLIVQEAVYVS
                     PRIDITDQVLKALAASGN"
     gene            2412..3515
                     /gene="lpxD"
                     /locus_tag="VHV99_23195"
     CDS             2412..3515
                     /gene="lpxD"
                     /locus_tag="VHV99_23195"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEX3638329.1"
                     /translation="MAFTLEDIVQRFGGEVVGEGSQRVGSLAPLDQAGPDQLAFLANP
                     KYLSQVETTRAGAVLINADDLAKLSSPEGRNFIVTPNPYAYFARVAQTFIDMAAPKAM
                     PGTHPSATIDPSAQIAPSAVIGPHVTVEAGAVIGENARLDANVVIGRGTRIGAGSHLY
                     PNVAVYHGCKLGERVIVHAGAVIGSDGFGFAPDFVGEGDARTGSWVKIPQVGGVSIAA
                     DVEIGANTTIDRGAMSDTIIEECVKIDNLVQIGHNCKVGAYTVIAGCAGIAGSTTIGR
                     HCMIGGAVGIAGHVTLADHVIVTAKSGVSKSLLKPGMYTSAFPAVNHADWNRSAALLR
                     NIDKLRDRIKVLENAASADKPANAASSSAGDKA"
     gene            3759..4223
                     /gene="fabZ"
                     /locus_tag="VHV99_23200"
     CDS             3759..4223
                     /gene="fabZ"
                     /locus_tag="VHV99_23200"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEX3638330.1"
                     /translation="MSTEKINLDIHKILTLLPHRYPILLVDRVLELEPHKSIKALKNV
                     SINEPYFQGHFPTRPVMPGVLILEALAQTAALLTFSEEPSDPSNTLYLFVGIDNARFK
                     RVVEPGDQLILNCTFERHMRGIWKFKARAEVDGVVAAEADLMCAVRHTDKDA"
     gene            4330..>4791
                     /gene="lpxA"
                     /locus_tag="VHV99_23205"
     CDS             4330..>4791
                     /gene="lpxA"
                     /locus_tag="VHV99_23205"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004193051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEX3638331.1"
                     /translation="MSRIHPTAIVEPGAQLDESVEIGPYAVIGAHVTIGARTTVGSHS
                     VIEGHTTIGEDNRIGHYASVGGRPQDMKYKDEPTRLVIGDRNTIREFTTIHTGTVQDA
                     GVTTLGDDNWIMAYVHLGHDCHVGNNVILASNAQMAGHVTIGDHAIVGGMSG"
BASE COUNT         1002 a         1431 c         1521 g          837 t
ORIGIN      
        1 tcgcggctac ctcgagttca acatcgactc gacccaggtg tcgatctcgc cggacaagaa
       61 ggacatgtat ctgacggtca cgctgcatga aggcgagccg tacacgatca gcagcatcaa
      121 gctggccggc aacctgctcg atcgcgagcc ggagctggcc aagctcatca agatcaaacc
      181 gggtgaccgt ttctcggctg aaaagctgca agccaccacc aagtcaatcg tcgacaagct
      241 gggcgaatac ggctatgcgt tcgccaccgt caacgcacag ccgcagatcg atcaggaaca
      301 ccacaaggtc gacctgacgc tgcaagtgga cccgagccgc cgcgtatacg tgcgccgcat
      361 caacgtggtc ggcaacacgc gtacgcgcga cgaagtggtg cgtcgcgaaa tgcgtcagct
      421 cgaaagctcg tggttcgatt caaaccgtct cgcgctgtcg aaagaccgga tcaaccgtct
      481 cggctacttt accgatgtcg acgtgaccac cgtgccggtg gaaggcacgc cggaccaggt
      541 cgatgtggac gtcaaggtgg ctgaaaagcc gaccggtgcg atcacgctgg gcgcgggctt
      601 ctcatcgacc gacaaggtgg tgctctcggc gggcgtgtcg caggacaacg tgttcggttc
      661 gggcacgagt ctcgcggtga acgtgaatac cgccaagacg taccgtacgt tgacggtcac
      721 gcaggtcgac ccgtacttca cggtcgacgg tatcaagcgg attaccgacg tctactaccg
      781 cacgtaccag ccgctttact actcgacgga ttcgagcttc aagatcgtca cgatcggtgg
      841 cgacctgaag ttcggtattc cgttctccga agtcgacacg gtgtacttcg gtgccggcct
      901 cgagcagaac cagctggata tcgacgcgaa cacgcctgct tcgtacatcc agtacgtcaa
      961 ggatttcggt cgcgtttcga acaacgtgcc gctcacggtg ggctggtcgc gcgacgcgcg
     1021 tgacagcgca ctggtgccga gccgcggtta cttcacgcag gcaaatgcag aatacggcac
     1081 gccgattggc ggcacgcagt actacaaggc cgacatcaac gcgcagtact attactcgtt
     1141 tgcgcgcggc ttcgtgctgg gcttcaactt ccagggcggt tacggtaacg gccttggcgg
     1201 caagccatac ccgattttca agaactacta cgctggcggt atcgggtcgg tgcggggcta
     1261 cgagccgagt tcgctgggtc cacgtgacaa gactacaggc gacccgattg gcggttcgaa
     1321 gatgctggtg ggcaatgtcg agttgacgtt ccctctgccc ggcacaggct acgacagaac
     1381 gttgcgcgta tttacgttcc tggatgcggg taacgtttgg gcggacgcga gcagtgcggc
     1441 cacgtcggtt ggttcgaacg gcctgcgtta cggctatggt gtcggtctcg cgtggatctc
     1501 accgatcggc ccgctcaagc tcagcctggg cttcccgctc acgaagcaca ccggcgacca
     1561 gtatcagaag ttccagttcc agatcgggac ggcgttctga tcaggcggcc ggcgaccggc
     1621 ggctggcctc gggcagccgg ccaaactaca gtatcgagag gatgactttg ctaaccggta
     1681 tgttttcgaa acgtgtggca tgtgcgttcg cgctggcaat gacactgggt gtcggcatgg
     1741 cgggggcagc gcacgcgcag gaagccagga tcgctgcggt gaattcggac cgcatcctgc
     1801 gtgaatcggc tgccgcgaag gctgcacagg tcaagctcca ggccgagttc gccagccgtg
     1861 acaaggatct cgccgataag actcaggcaa tcaaagcact tgccgatgaa cttgataaga
     1921 agggcgcgtc gatgcagccc gaggtgcgtg cacagaagca gcgaacattg tcgcaaatgg
     1981 attttgacct tcagcgctcg caacgcgagt ttcgcgacga cctggagcgt cgccgcaacg
     2041 aagagctggc ggcggtgctc gaccgggcca acaaggtgat caagcagatc gccgagcagc
     2101 agcactatga cctgatcgtt caggaagcgg tgtacgtgag cccgcgcatc gacattaccg
     2161 atcaggtgct caaggccctg gctgcctcgg gtaactgaac gcgcccgctg ggtcgccggc
     2221 tatcggatcg ccggccgggg cggtagaacc gacagtttgg actgatttgc agcaacgagc
     2281 ttgcatggga ttggagtggc gctgggggac cagagtaagc gctaaagcgc taactctggt
     2341 cgacagcgtt gcatgggacc agagtaagcg ctaaagcgct aactctggtc gacacaggag
     2401 acagtatagg catggcattt acgctcgagg acatcgtcca acggttcggc ggtgaagtag
     2461 tcggtgaggg ttcgcagcgc gtcggcagtc tggcgccgct cgaccaggca ggcccggacc
     2521 agctggcgtt cctcgccaat ccgaagtacc tgtcgcaggt cgagacgacc cgcgcgggcg
     2581 cggtgttgat caacgccgac gacctggcga agctcagttc ccctgaaggc cgtaacttca
     2641 tcgtcacgcc gaatccttac gcctacttcg cgcgcgtcgc gcagactttc atcgacatgg
     2701 cggcgcccaa ggccatgccc ggcacgcacc cgagtgcgac gatcgacccc tcggcgcaaa
     2761 ttgcgccgag cgcggtgatc ggcccgcacg tcacggtgga agcgggcgcg gtgattggcg
     2821 agaacgcgcg cctggatgcc aacgtggtga tcggccgcgg cacgcggatc ggcgcgggct
     2881 cgcatctgta tccgaatgtc gcggtgtatc acggctgcaa gctcggcgag cgcgtgatcg
     2941 tgcacgcggg cgcggtgatc ggttcggatg gttttggctt cgcgccggat tttgtcggcg
     3001 aaggcgatgc gcgtaccggc agctgggtaa aaattccgca ggtgggcggt gtgtcgatcg
     3061 cggcggacgt cgaaattggc gcgaacacta cgatcgaccg tggcgctatg tccgacacga
     3121 tcatcgaaga gtgcgtgaaa atcgacaatc tcgtgcagat cggtcacaac tgcaaggtcg
     3181 gcgcttacac ggtgatcgcc ggctgcgcgg gcatcgcggg cagcaccacg atcggccgtc
     3241 attgcatgat cggcggcgcg gtcgggatcg cggggcacgt caccctggct gatcatgtga
     3301 tcgtcacggc gaagtcgggt gtgtcgaagt cgttgttgaa gcccggcatg tacaccagcg
     3361 cgtttccggc tgtgaatcac gcggactgga acaggagtgc cgccttgctg cgtaacatcg
     3421 acaaactccg cgatcgtatc aaggtgctcg aaaacgccgc ttcggccgac aagccggcca
     3481 acgcagcaag cagttccgca ggcgacaaag cctgagtaac cggattaacc cgcagagcac
     3541 ccgcagaacg tccgcaggcc gactcgggga acaattccgc caggcaaccg gcggctaccg
     3601 cgtaaaatag cgggcgagca tcaggaagca aagtcggttg ccgtgaccgg gtggcatggc
     3661 cgccggattt cggcaagcgc cggcagcacg cgttaccaac ccacctgcac cagcatccgc
     3721 agtcatcatc gcgcagtcaa tgcgtgagta gaaacaccat gagcaccgaa aaaatcaatc
     3781 tcgacattca taagattctc acgctgctgc cccatcgtta cccgatcctg ctggtcgacc
     3841 gggtgctcga actcgagccg cataagagca tcaaagcgct gaagaatgtg tcgatcaatg
     3901 agccgtattt ccagggtcac tttccgaccc ggccggtgat gcccggcgtg ctgattctcg
     3961 aagcgctcgc gcaaacggcg gccctgctga cgttttcgga agagccgagc gatccgtcga
     4021 acacgctgta tctgtttgtg ggcatcgaca atgcgcgctt caagcgcgtc gtggaaccgg
     4081 gcgatcagtt gatcctgaac tgcacgttcg agcgtcatat gcgcggcatc tggaagttca
     4141 aggcacgcgc cgaagtggat ggcgtcgtgg cggcggaagc cgacctgatg tgcgcggtgc
     4201 ggcacacgga caaagacgct tgaagatcgg cgtccgcgcg gccggcgact tgctcagcgg
     4261 cgtagttaag cccggcaagc tccgcccaga cccacccaga caacgcaaca gaatcagaag
     4321 cgaggacgca tgagcaggat tcatcccact gcgatcgtcg aaccgggcgc acaactcgac
     4381 gaatccgtcg aaatcggacc gtatgccgtg atcggcgcac atgtgacgat cggcgcgcgg
     4441 accacggtcg gttcgcacag cgtgatcgaa ggtcacacta cgattggcga agacaaccgg
     4501 attggccact acgcatcggt cggcggccgt ccgcaggaca tgaagtacaa agacgagcca
     4561 acccggctcg tgatcggcga tcgcaacacg atccgggaat tcaccacgat ccacaccggc
     4621 acggtacagg acgcgggcgt caccacgctg ggcgacgaca actggatcat ggcctacgtg
     4681 cacctcgggc acgactgcca cgtcggcaat aacgtcatcc tggcgagcaa tgcgcagatg
     4741 gccggccacg tgacgatcgg cgaccacgct atcgtcggcg gcatgtcggg c
//
LOCUS       DATASH010000869         3427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5290232,
            whole genome shotgun sequence.
ACCESSION   DATASH010000869 DATASH010000000
VERSION     DATASH010000869.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5290232"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /gene="cysW"
                     /locus_tag="VHV99_23210"
     CDS             <1..653
                     /gene="cysW"
                     /locus_tag="VHV99_23210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HEX3638332.1"
                     /translation="VFGLVASWCIAKFEFRGKALLTTLIDLPFSVSPVISGLIYVLMF
                     GAQGWFGPWLEAHNVQIIFAVPGIVLATIFVTFPFVARELIPLMQAQGNDEEEAAHVL
                     GASGWQIFRRVTLPNVKWGLLYGVILCNARAMGEFGAVSVVSGHIRGQTDTMPLHVEI
                     LYNEYNFSAAFAVASLLALLALVTLALKLLAERRMSQSIEAVQARPADIPVASIQN"
     gene            669..1727
                     /locus_tag="VHV99_23215"
     CDS             669..1727
                     /locus_tag="VHV99_23215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014897109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HEX3638333.1"
                     /translation="MSIIVRNLQKRFGDFVALDNVSLDFPSGELVALLGPSGCGKTTL
                     LRVIAGLEYADAGSVVLNGEDVAAVGARERQVGFVFQHYALFRHMTVFENVAFGLRVK
                     PRKERPSEAVIREKVHELLKLVQLDWLAQRFPSELSGGQRQRIALARALAVEPKVLLL
                     DEPFGALDAKVRKELRSWLRRLHDDLHISTIFVTHDQEEALEVADRIVVFNRGHVEQV
                     GSPQDVYDHPQTSFVYEFLGAANRLKGSVDANGFVAQGAAQPIATEANFVGPAFAYVR
                     PHDLTLFPTASGHRDGIVVDVRRVVTLGGSVRVDLEGAEGNVLEAELDRETWRGLNLN
                     IGDGVTAVPRVLRVFPAH"
     gene            1752..2678
                     /locus_tag="VHV99_23220"
     CDS             1752..2678
                     /locus_tag="VHV99_23220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011884743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HEX3638334.1"
                     /translation="MNFQQLRFVREAVRQNMNLTEVANVLYTSQSGVSKQIKDLEDEL
                     GVDIFIRRGKRLTGLTEPGKEVHQVIERMLLDAENLRRVARQFADQDNGHLVVATTHT
                     QARYALPKVIRQFTEVFPKVHLALRQGSPQQIAQMIINGEADIGISTEALDRYPDIVT
                     FPCYTWHHTVVVPKGHPLVGRANITLDEIAEYPIVTYDHDFTGRSHVDQAFAKAGALP
                     DVVLTAIDADVIKTYVELGLGIGIVAAMAFDEKRDTELVALDTQHLFEASTTRVGLRK
                     GAFLRAYAYRLIEMFAPQLDEAQIAAQLREAV"
     gene            2739..>3427
                     /locus_tag="VHV99_23225"
     CDS             2739..>3427
                     /locus_tag="VHV99_23225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008349891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEX3638335.1"
                     /translation="MNSSHTSLPRIAVLATGGTIAGEAGDAAKTSGYKAGVVGVDKLL
                     GAVPSLSKVAQIQAEQVASIDSKDMTPALWATLCARVNTLLVRADIDGIVITHGTDTL
                     EETAYLLHLTVKSGKPVVLTAAMRPATALSADGPLNLLNAVTVAASRAAWKQGVLIAF
                     NNQIHCARDVTKTSTYAVDAFRSPESGALGWVQDGQVEFQRSVVRPHTLHSPFQMAAD
                     LPSVEIVASYAG"
BASE COUNT          650 a          989 c         1112 g          676 t
ORIGIN      
        1 tggtgttcgg cctcgtggct tcgtggtgta tcgccaagtt cgagttccgc ggcaaggcgc
       61 tgctgaccac actgatcgat ctgccgttct cggtttcgcc ggtgatttcc ggcttgatct
      121 acgtgttgat gttcggcgcg cagggctggt tcggtccctg gctcgaagcg cataacgtgc
      181 agatcatctt cgcggtgccg ggcatcgtgc tggcgacgat cttcgtcacg ttcccatttg
      241 tcgcacgcga gctgatcccg ctgatgcagg cgcaaggcaa cgacgaagaa gaagctgcgc
      301 acgtactggg cgcgtcgggc tggcagatct tccgccgcgt gacgctgccg aacgtgaaat
      361 ggggtttgct atacggcgtg attctctgca atgcacgggc gatgggcgag ttcggcgcgg
      421 tatcggtggt atcgggtcat atccgcggac aaaccgacac catgccgctg cacgtagaga
      481 ttctctacaa cgaatacaac ttctcggcgg cgtttgccgt ggcgtccctg cttgcgctgc
      541 tcgcgctcgt cacgctggcg ctcaagctgc tggccgagcg ccgcatgtcg caatcgattg
      601 aagcagtgca ggcgcggccg gccgatattc cggtcgcgtc aatccagaac tagaaggcga
      661 aaatcaccat gagcatcatc gttcgcaatt tgcagaaacg cttcggcgat ttcgtcgcgc
      721 tggataacgt ctcgctcgat ttcccatcgg gcgagctcgt cgcgctgctc gggccgtcgg
      781 gctgcggcaa gaccacgctg ctgcgcgtga tcgcggggct ggaatacgcg gacgccggtt
      841 cagtcgtgct gaacggcgag gacgtggcgg ccgtgggcgc gcgggaacgg caggtcggct
      901 ttgtgttcca gcattacgcg ctgttccgtc acatgacggt gttcgaaaac gtggcgttcg
      961 gcctgcgcgt gaaaccgcgc aaggagcggc cgtccgaagc ggttatccgc gagaaagtgc
     1021 atgaattgct gaagctcgtg caactcgact ggctggcgca acggtttcca tcggagcttt
     1081 caggtggaca gcggcagcgg atcgcgctgg ctcgtgcgct ggcagtcgaa ccgaaggtgc
     1141 tgctgctcga cgagccgttc ggcgcactcg atgccaaggt ccgaaaggag ttgcgtagct
     1201 ggctgcggcg tctgcatgac gacctgcata tctcgacaat tttcgtcacg cacgatcagg
     1261 aagaagcgct tgaagtcgcg gaccggatcg tggtgttcaa tcgtgggcat gtagagcagg
     1321 tcggcagtcc gcaagatgtc tatgaccatc cgcagacatc gtttgtctat gaattccttg
     1381 gtgcggcaaa ccggctcaag ggcagcgtgg atgcgaacgg atttgtggcg caaggcgccg
     1441 cgcagccgat cgccaccgaa gcgaacttcg tgggtccggc gttcgcttac gtgcggccgc
     1501 acgacctgac actgttcccg acggcttccg gtcatcgcga tggcatcgtg gtcgatgtcc
     1561 gacgcgtcgt gacgctcggc ggctcggtgc gcgtggacct ggaaggcgcc gaaggcaacg
     1621 tgctggaagc ggaactggat cgcgaaacct ggcgtggatt gaacctgaat atcggcgacg
     1681 gcgtgactgc cgtgccgcgt gttctgcgcg tgttcccggc gcattgagct cactaaaaaa
     1741 gagaggctga catgaacttc cagcaattgc gttttgtacg cgaagccgta cgccagaaca
     1801 tgaatctgac cgaggtcgcg aacgtgcttt atacgtcgca gtcgggtgta tcgaagcaga
     1861 tcaaggatct cgaggacgaa ctcggcgtcg atatcttcat tcgacgcggc aagcgtctca
     1921 ccggcctgac cgagccgggc aaggaagtgc atcaggtgat cgagcgcatg ctgctcgatg
     1981 ccgagaatct gcgtcgtgtt gcccgccagt tcgccgatca ggacaacggc catcttgtgg
     2041 tggcgaccac tcacacgcag gcgcgttacg cgctgcccaa ggtgatccgc cagttcaccg
     2101 aggtattccc gaaggtccat ctcgcgctgc gtcagggcag tccgcaacag atcgcgcaga
     2161 tgatcatcaa tggcgaggcg gacatcggca tctcgacgga agcgcttgac cggtatccgg
     2221 acattgtgac gttcccgtgc tacacgtggc atcacacggt ggtcgtgccg aaggggcatc
     2281 cgctggtcgg tcgcgcgaac atcacgctcg acgagattgc tgaatatccg atcgtcacgt
     2341 atgaccacga tttcaccggc cgctcgcatg tcgatcaggc atttgcgaaa gcgggggcgc
     2401 tgccggacgt cgtcctgacg gctatcgatg ccgacgtcat caagacgtat gtggaactgg
     2461 ggctgggcat cggcattgtg gcggcgatgg ccttcgacga aaaacgcgac accgaactcg
     2521 tcgcgctcga cacgcagcat ctgttcgaag ccagcaccac gcgggtgggt ttgaggaagg
     2581 gcgctttcct gcgggcatac gcgtacaggc tgatcgagat gttcgcgccg caactcgatg
     2641 aagcgcagat cgcggcgcaa ttgcgggaag ctgtctagcc cttcaaaggc ttaagcgcgt
     2701 aagaatcagc aagccgccgc tccgctacaa ttacggccat gaactcctca cacacttcgt
     2761 tgccgcggat cgcggtgctg gctaccggcg gaaccatcgc gggcgaagcc ggcgatgccg
     2821 cgaaaacctc gggttacaag gccggcgtag tcggcgtcga taaactgctc ggtgccgtgc
     2881 cttcgctctc gaaagtagcg cagattcagg ccgagcaagt agcgagtatc gacagcaagg
     2941 acatgacccc ggcgctatgg gccacgctct gcgcgcgagt gaatacgttg ctggtgcggg
     3001 ccgatattga cggcatcgtc atcacgcacg gcaccgatac gcttgaagag accgcctacc
     3061 tgctgcatct caccgttaaa agcgggaaac cggttgtgct gacggcggcc atgcggccgg
     3121 cgacggcttt atccgctgat ggcccgctga atcttctcaa cgcggtgacg gtagcggcga
     3181 gccgggcggc atggaagcag ggcgtactga tcgcgttcaa caaccagatc cactgtgccc
     3241 gcgatgtgac caagacaagt acctatgccg tcgatgcctt ccggtcaccc gaaagcggcg
     3301 cgcttggctg ggtgcaggat ggccaggtgg aattccagcg ttcagtggtg aggccgcata
     3361 cgctgcatag cccgttccag atggcggctg atctgccttc ggtcgagatc gtggcgagtt
     3421 atgcggg
//
LOCUS       DATASH010000870         4713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2915873,
            whole genome shotgun sequence.
ACCESSION   DATASH010000870 DATASH010000000
VERSION     DATASH010000870.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4713
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2915873"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..974
                     /locus_tag="VHV99_23230"
     CDS             <1..974
                     /locus_tag="VHV99_23230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3638336.1"
                     /translation="KFSRDPRAPNLVDNVSFSVSAGETLCIVGESGCGKSVTSLALMG
                     LLPSPPANVVAGAAHFDGKDLFRQPERELADIRGNRLSMIFQEPMTSLNPAFTVGEQI
                     GESIRRHRDVERGQAREQALEMLRLVRIPAPETRLDAYPHQLSGGMRQRVMIAMALAN
                     RPRLLIADEPTTALDVTIQAQVLSLVKNLQAETGTAMVLITHDLGVVAEVADHVAVMY
                     AGRIVEYGTVADLFDDPQHPYTIGLMGAIPSVGKREGALATIRGSVPSPELMPHGCRF
                     APRCPFAEQRCIDAAPPDKVVAGVHRVACWLAPVEQVAAPLVRKEIA"
     gene            971..2101
                     /locus_tag="VHV99_23235"
     CDS             971..2101
                     /locus_tag="VHV99_23235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX3638337.1"
                     /translation="MTTPTTPTPHAQPASNAPAPIIEARSLTKHFGGTRQLFTRTPTV
                     YAVNDVSFAVQPGETFAIVGESGCGKSTLGRLLLRLIDSTDGRVFYQGKDITRQQGAP
                     LRRLRREMQIIFQDPFASLNPGMTIGQIVGEPIALHGLARNHAEHRERVAQLLRTVGL
                     QPAYADRYPHEFSGGQRQRIGIARALAGEPKLIVGDEPVSALDVSVQAQVINLLETLK
                     QDLGLTLIMVAHDLAVIRHMSDRVAVMYLGEIVELADVDALFDAPLHPYTQALLRAIP
                     ASSPHERRAKPALSGDLPSPTAPPSGCRFHTRCPHAKPHCAQARPLSERLDDGRQVAC
                     HFWREIQDAGGGAPLATSVSPKLIERLAIYRDRQTALAAAGD"
     gene            2204..3706
                     /locus_tag="VHV99_23240"
     CDS             2204..3706
                     /locus_tag="VHV99_23240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3638338.1"
                     /translation="MRRMLIAAALALSASAAMAQTAQTIRIGLQDDIGSLDPARSVQL
                     VDRIVFASMCNALVDITPDLKIVPMLATSWTTSADGKTLTFKLRQGVKFQDDEPFNAA
                     AVKANLDRDRTLQTSNRKSELASVDHVDVIDDYTVAIVLKQPDAALLATLTDRAGMML
                     APKTFADPAGVAAHPVCSGPYKFVQRVQNDRVVLEKFPGYWDADKYPIQRVVFLPIPD
                     STVRLANLRSGSLDMLERLAPSDVASAKSDTNNKFAPVSGFGYYYVTFNIGNGKRSNA
                     VLRDKRVRQAFELAIDRDAINQVIGAGIFAPANQAVPKSSPYYNASLPMQHRDIAKAK
                     ALLKAAGHEHVDIEFTYPNNTVSSQIVQMMQAMVSEAGFNLKLRPTDYATELNAAHSG
                     DFEAMYLGWSGRVDPDGNLHQFNTCAGNLNYAHYCNPAVDQLLDAARVKLTTAERKTE
                     YDAASKLLADDDPIIYVYNQPWPFELSKKVQGFTPYPDGLIRLRGVSIKG"
     gene            3713..4657
                     /locus_tag="VHV99_23245"
     CDS             3713..4657
                     /locus_tag="VHV99_23245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019247679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3638339.1"
                     /translation="MLQIIANRVLVAIPTLILVSMLIFGLQKLLPGDPVTAMAGEDQD
                     PQLIASLRVKYHLDEPVPTQYALWVRDVVHGDLGASLRTDVPVTSLIAQKLPVTLQLA
                     AMAMIFAIGIGIPLGVISAASRGGALDYGANVFALSGMSIPNFWLGIMLIFIVSVRWH
                     LLPSSGYVSPAEDFWLSIKTMLMPAFVLGASLGAQLMRHTRSAMLSVLRTDYIRTARA
                     KGLLRGTVVVKHAFRNALVPIVTVLALLFGELLAGAVLTEQVFTIPGFGKLIVDAVFN
                     RDYAVVQGVVLVTALCFIVVNLCADVLYVLLNPRLRRS"
BASE COUNT          814 a         1570 c         1514 g          815 t
ORIGIN      
        1 tgaagttttc gcgcgatcca cgcgccccga atctcgtcga caacgtctcg ttttcggtga
       61 gcgcaggcga aacgctgtgc atcgtcggcg aatcggggtg cggcaagagc gtgacgtcgc
      121 tcgcgttgat gggcctgctg ccgagcccgc cggcaaacgt tgtcgcaggc gccgcgcact
      181 tcgacggcaa ggatctcttc aggcagcccg agcgcgaact ggccgacatt cgcggcaacc
      241 ggctgtcgat gatctttcag gagccgatga cttcgctgaa tccggcgttc acggtcggcg
      301 agcagatcgg cgaaagcatc cggcggcatc gcgatgtcga gcgcgggcag gcgcgcgaac
      361 aggcgctgga gatgctgcgg ctggtgcgta tccccgcgcc ggaaacccgg ctcgatgcgt
      421 acccgcatca gctttccggc ggcatgcgcc aacgcgtgat gatcgcgatg gcgctcgcca
      481 accgcccgcg tctgttgatc gccgacgagc cgacgaccgc gctcgacgtg acgatccagg
      541 cgcaggtgct gtcgctcgtg aaaaatctgc aagccgagac cggtaccgcg atggtgttga
      601 tcacgcacga cctcggcgtc gtcgcggaag tcgccgacca cgtggcggtg atgtacgcgg
      661 gacgcatcgt cgaatacggc accgtcgccg atctgttcga cgacccgcaa catccctaca
      721 cgatcggcct gatgggcgcg attccgtcgg tcggcaagcg cgaaggcgcg ctcgcgacga
      781 tccgcggttc ggtgccgtcg cccgagttga tgccgcacgg ctgccgcttc gcgccgcgct
      841 gcccgttcgc cgagcaacgc tgcattgacg ccgcgccgcc cgacaaggtg gtcgccggcg
      901 tgcatcgcgt tgcgtgctgg ctcgcgccgg tcgaacaggt cgccgcgccg ctcgtgcgca
      961 aggaaatagc atgacgacgc ccactacacc gacgccgcac gcacagccgg cctcgaatgc
     1021 gccggcgccg atcatcgagg cgcgctcgct gacgaagcat tttggcggta cgcggcagct
     1081 cttcacgcgc acgccgaccg tctatgcagt caacgacgtg tcgttcgcgg tgcaacccgg
     1141 cgagacgttc gcgatcgtcg gcgaatccgg ctgtggcaag tccacgctgg gccgcctgct
     1201 gctgcgtttg atcgactcga cagacggccg cgtcttctat caaggcaagg acatcacgcg
     1261 tcagcaaggc gcgccgctgc ggcggctgcg ccgcgaaatg cagatcatct ttcaggatcc
     1321 cttcgcgtcg ctgaatccgg gcatgacgat cggccagatc gtcggcgagc cgatcgcgct
     1381 gcacgggctc gcgcgcaacc acgccgagca ccgcgagcgc gtcgcccaac tgctgcgcac
     1441 ggtcggcctg caaccggcct acgcggaccg ttacccgcac gaattctccg gcggccagcg
     1501 gcaacggatc ggcatcgcgc gcgcattggc cggcgagccg aagctgatcg tcggcgacga
     1561 gccggtgtcg gcgctcgacg tctccgtgca ggcgcaggtg atcaatcttc tcgagacact
     1621 gaagcaggac ctcggcctca cgctgatcat ggttgcgcat gacctcgcgg tgatccgcca
     1681 tatgagcgac cgcgtcgccg tgatgtatct cggcgagatc gtcgagctgg ccgatgtcga
     1741 tgcactgttc gacgcgccgc tgcatccgta cacgcaagcg ctgctgcgcg cgattccggc
     1801 gagcagcccg cacgagcggc gtgccaagcc tgcgttgtcc ggcgatctgc cgagtccgac
     1861 cgcgccgcct tccggttgcc gttttcatac gcgctgtccg catgcgaagc cgcactgcgc
     1921 gcaagcgcgg ccgctcagcg aacgactcga cgacggacgg caggtcgcgt gccacttctg
     1981 gcgcgagatc caggacgccg gcggcggcgc gccgctggcc acgagcgtca gcccgaaatt
     2041 gatcgagcgg ctcgcgatct accgcgaccg ccagacggcg ctggccgccg ccggcgactg
     2101 agccggtggt cgagatgaac ttcgagtgaa gaaccgaggc gcgccgcgcc gctgcccctt
     2161 cacgacaccg ttttatgaat tggcatacaa agggaaagtc ctcatgcgaa gaatgttgat
     2221 tgccgcggcg ctcgcactca gcgccagcgc cgcgatggcc cagaccgctc agacgatacg
     2281 gatcggcttg caggacgaca tcggttcgct cgatcccgca cgcagcgtgc agctcgtcga
     2341 ccggatcgtg tttgcgtcga tgtgcaatgc gctggtcgat atcacgcccg acctgaagat
     2401 cgtgccgatg ctggcaacct cgtggacgac gagcgccgac ggcaagacgc tgacgttcaa
     2461 gctgcgtcaa ggggtgaagt tccaggacga cgagccgttc aacgcggccg ccgtcaaggc
     2521 caacctcgac cgcgaccgca cgctgcagac gagcaaccgc aagagcgaac ttgcgtcggt
     2581 cgatcacgtc gacgtcatcg acgactacac ggttgcaatc gtgttgaagc agccggacgc
     2641 cgcgctgctc gcgacgctga ccgaccgcgc cggcatgatg ctcgcgccga agacgttcgc
     2701 cgatccggcc ggcgtcgccg cgcatccggt gtgttcgggt ccgtacaagt tcgttcagcg
     2761 cgtgcagaac gatcgcgtcg tgctcgagaa attccccggc tactgggacg ccgacaaata
     2821 cccgatccag cgcgtggtct tcctgccgat tccggacagc accgtgcgcc tcgccaatct
     2881 gcgttcgggt tcgctcgaca tgcttgagcg cctcgcgccg tccgacgtcg cgtcggccaa
     2941 gagcgacacg aacaacaagt tcgcgccggt cagcggcttc ggctactact acgtgacgtt
     3001 caacatcggc aacggcaagc gcagcaacgc ggtgttgaga gacaagcgcg tgcggcaggc
     3061 attcgaactc gcgatcgacc gtgacgcgat caaccaggtg atcggcgcgg gtatcttcgc
     3121 gccggcgaat caggcggtgc cgaagtcgag cccgtactac aacgcgtcgc tgccgatgca
     3181 gcatcgcgat atcgcgaagg cgaaggcgct gctgaaggcg gcggggcacg agcacgtcga
     3241 catcgagttc acttacccga acaacacggt gtcgagccag atcgtgcaga tgatgcaggc
     3301 gatggtcagc gaagccggct tcaacctgaa gctgcggccg accgactatg cgaccgaact
     3361 caatgccgcg cacagcggcg acttcgaagc gatgtacctc ggctggtcgg gccgggtcga
     3421 tccggacggc aacctgcacc agttcaacac gtgcgccggc aacctcaatt acgcgcatta
     3481 ctgcaacccg gcagtcgacc agttgctcga cgccgcgcgc gtgaaactga ctaccgcgga
     3541 gcgcaaaacg gagtacgacg cggcctcgaa gctgctcgcc gacgacgatc cgatcatcta
     3601 tgtctataac cagccgtggc cgttcgagct gtcgaagaag gtacaaggct tcacgcccta
     3661 tcccgacggc ctgatccgcc tgcgcggcgt cagtatcaag ggctgaccga tcatgctgca
     3721 gatcattgca aatcgcgtgc tggtcgccat tccgacgctg attctcgtgt cgatgctgat
     3781 ctttggcctg cagaagctgc tgcctggcga tccggtgacg gcgatggccg gcgaagatca
     3841 ggatccgcag ttgatcgcgt cgctgcgcgt caagtaccac ctcgacgagc cggtgccgac
     3901 gcagtacgcg ctgtgggtcc gtgacgtcgt gcatggcgat ctcggcgcgt cgctgcgcac
     3961 cgacgtgccg gtgacgtcgc tgatcgcgca gaagctgcct gtcacactgc agctcgcggc
     4021 gatggcgatg atctttgcaa tcggcatcgg cattccgttg ggcgtcatct ccgccgcaag
     4081 ccgcggcggc gcgctcgatt acggagcgaa cgtcttcgcg ctgtccggca tgtcgatccc
     4141 gaacttctgg ctcgggatca tgctgatctt cattgtgtcg gtgcgttggc atctgttgcc
     4201 gtcgtccggc tatgtgtcgc cggccgaaga cttctggctc agcatcaaga ccatgctgat
     4261 gcccgcgttc gtgctcggcg cctcgctcgg cgcgcaattg atgcgccaca cgcgcagcgc
     4321 gatgctgagc gtgctgcgca ccgactatat tcggaccgcg cgtgccaaag ggctgctgcg
     4381 cggaacggtc gtcgtcaagc acgcttttcg caacgcgctg gtgccgatcg tcaccgtgct
     4441 cgcgctgctg ttcggcgaac tgctcgcggg tgcggtgctg accgagcagg ttttcacgat
     4501 ccccggcttc ggcaagctga tcgtggatgc cgtgttcaat cgcgactacg cggtcgtgca
     4561 gggtgtcgtg ctcgtcaccg cgctatgctt catcgtcgtg aacctgtgcg ccgacgtgtt
     4621 gtacgtcctc ctcaatcccc gactgagacg cagctgatgg ctactcccgc cgcccccgtt
     4681 cacgcatcgg ccgctgcgtc gcaggcgacc gta
//
LOCUS       DATASH010000871        20631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_148953,
            whole genome shotgun sequence.
ACCESSION   DATASH010000871 DATASH010000000
VERSION     DATASH010000871.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 20631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20631
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148953"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2722
                     /locus_tag="VHV99_23250"
     CDS             <1..2722
                     /locus_tag="VHV99_23250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638340.1"
                     /translation="QAAADLNTIAAHTSGTINATAATTITGIATDVAAAVTAGDIILS
                     GTEAVTTTDASQAAADLNTIATYTSGAVNAAAASTVTGLVADLETVVASTNISLSGSE
                     TLSVTDPTVTMAELNALAALTLGTVDADAVLTVTGSVTDIETALASNRITFSAAESFT
                     VQDTTVAASDLNTIAGYTSGTVDATAATTIAGSAADVATALAAIDIRLSGTEIVAISD
                     TAAAAADLKAIAADTSGQVTATSVITLSGSAADVAAVVIASNISLSDAESVTISGTTA
                     AAADLNTIVNNTSGTVDAMALGTITGSAADLETALASTSITFAGNENLTLSDTSVAVS
                     VLSTLAGETTGTVNATAATLVTGTAAAVAAAVIAADITLSGSEAVTTSDATQAAGDLN
                     TIAANTSGTVNGAAATTITGSAVALNALLISSNIVTSGTENITVSDASVAASDLSAIA
                     GHTTGTVNAEATTITGSASAIIAAHSTSNISISSSAVYTVTGTATVSQVSTITGYTTG
                     AVAYSIYDSVANLSTSNAHYALLLAAATVTAYLDSSHNNLTLVSLDNGVGSIDLNGQA
                     ATLTNSELSLNIVDSVGGATVKEVATSNLDLSAGVDSYVNSLDLAGHTVKLTASEISG
                     LTLVDSVGGGTVTEKLVADTNLTGGVDSHVSNIDMAGHVVTLSASELATLSLSDSVGG
                     GYVIEQLQGSTNLTTGLNSHVQEIDLAGYTVKLTDTELASGLAHGVMDSSQGHTGSVV
                     EQFTTSVDLTLTSSTLNPLVHGLDLNGNSVTLTDTQLGQINFVNDTLGGGSVTESDTH
                     ALNNGDTLSLVTHSTTTLNLDLNNEVAANATVTIAGLQHNDTLNVGAGSTLTSAVGSA
                     QTVNQAGEYFFDTSAKTLTWDDSSSHVHSVVLANVTSVTLEPNQHTFTVI"
     gene            2871..4154
                     /locus_tag="VHV99_23255"
     CDS             2871..4154
                     /locus_tag="VHV99_23255"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX3638341.1"
                     /translation="MFLKNYMAGAVATALVTASLGASAADWSLGQMARQALATHPEIV
                     GKQYSLNAAEANVKAASWQRFPTLAVEGGKANDGNPRFTDISLTQPIWSGGRISGTIA
                     SAEAQKDAASASVDETREDILLRVATAYVEAMRQQARTAIAEKEVEQHQQLFDMISRR
                     VDAQASATVDRDLAQSRLYQADNDLSTAKQALATALSQLSQLVGQRVPAVDDYSIDQM
                     MPDSVDTAVDLATGVSPTLKRYDYQEKAAEADITVKKAAYLPQVSVRFDKFYGQDLIT
                     PQDTTRGSRVMLVIQAEPGAGLSAAAGVGAAVAAKNAAIEARTAALRDLRLLVSTDWD
                     EYMGARERFQSSTLSTASSKAVYESYVRQYTIGKKSWLDVMNTVREASQSEMTTADAK
                     AQMTAAALRLEVRTGETPFLDEASNVPPAQNNSTP"
     gene            4151..6268
                     /locus_tag="VHV99_23260"
     CDS             4151..6268
                     /locus_tag="VHV99_23260"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3638342.1"
                     /translation="MNPLLSAITLAAKAVGQTVPQALLQDVQTVLEVGADAPVLERVR
                     AAWQAGSLTGEVYELAAPGAGQGPFLARAASGEWWIVLARNADGSWTARAADGQIARL
                     DGLEGAVCIGLPVEHDDAFGGSDPADPSSPNTSAKRLVLQALWRNKSHYIEAILATVL
                     TNILAITTSLFSMQVYDRVIPNRGFATLVVLSVGVGAAILLELLLKHVRGASIERTAR
                     RIDESLSVWFYNRLINIRLEHRPSSIGSLASQVKGFESVRAILSSTSLFVLVDIPFAL
                     LFILIIGLIGGRLVLVPLLMFPISLCAGLMFQRQIAFHSRQNQGQSHRKAGLLVESID
                     GAESFKANGADWSLQRRWSGLVDDAGASDYKVKNYSSLSQHLTVALQQLGYVGLVAFG
                     AYLVADNKLTMGALIACTIISGRAMSPIAQLPATLVQWSNARAAANGLDKLICLPNDN
                     DARGHALVPEIVDGAMRIDGVKFSYGHGGDAAVDIEQLDIKTGERIGIIGSIGSGKST
                     LLKLMSGLFRPAAGRISINGIDMALILPEVLHEKIAYLPQEIRLISGTLRGNLVQGLP
                     DPGDERLLEVARLTGLMDLITRHPKGLALPITEGGRGISGGQRQLIGLTRLLLMRPKV
                     LVLDEPTASMDSATEKRIVAVIDAIAARGTTVLVATHKTALLPVTRRLLVMRDGRVVM
                     DGPRDQVIDKLFGTSPVASTSEV"
     gene            6277..7440
                     /locus_tag="VHV99_23265"
     CDS             6277..7440
                     /locus_tag="VHV99_23265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HEX3638343.1"
                     /translation="MSDITQEIPRARWALTACCGVAIVGFIVWARWASLDQITRAQGQ
                     VIASSRDQIIQSQDLMTIDAVLVKEGADVKRGQPLVRFERTRAEAAYLESQSKEMSLE
                     AEVARLHAEVYGGTPQFPKSLDAYSDIRSNEELLFRKRQAAVDDEVAAMRKSLEYVQD
                     ELNINLPLLASGDVSRADVLKLERQVADLQGQITNRRNKYMQDSQTDLSKALEDLAGV
                     DQVVAQRKDQLDHTVIDSPVDGIVRNVRISTPGGVARAGDEIMQIVPVDDELIIEAKV
                     KPKDIAFIKPGLPAKIKLDAYDYTIYGTLSGTVTYISADTLNDDTHPANEQPFYRVQV
                     KTNGNDLGRAVGHRISVQPGMTATVEIKTGSNTVLNYLTKPITKTVTESMGER"
     gene            complement(7501..8544)
                     /locus_tag="VHV99_23270"
     CDS             complement(7501..8544)
                     /locus_tag="VHV99_23270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX3638344.1"
                     /translation="MNLGANPIQVHSPLKPGIAHSHFDVRTKPPQRQLLAWRDRVGHV
                     IDVLPSLSQLDEPFQASIDRYQIGQLAFTDCRSDRMMLDRSQARISTDKVRDVAFHVF
                     LEGSVENITLRATPPKSAPAPAATILALDLNQPVRMQRNACRVLTLFVPSETVQAEFP
                     DAEAIHGRAIHGTTPLTRLVVDHLSALSQNIAQMSAESADEAIRSGAQMLIALFGKQA
                     LLSGNARAAGRAAMFGQARRYIQANRHNAALSPESVMSALGLPRQTLYRLFQHEGGVG
                     AYIRHIRLRHAADDLIRKPQTAVANIGYELGFKSATDFTRAFRRAYGMTPLDFRTLTA
                     QDRRSAVKPVRRT"
     gene            9147..9791
                     /locus_tag="VHV99_23275"
     CDS             9147..9791
                     /locus_tag="VHV99_23275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007584238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638345.1"
                     /translation="MKNGKPKFTRQHADGLFPDLKPSGAHIGSRPDNPFGGEAFEAVD
                     RHDRDEKSLWKENLVTLPMGIELPPGYESASRVREAIARAWRMKWVREARNEIVAEFP
                     EGWSAVRPKSGPVELRDANGVVRALYGWAEDAELRILPRYLVESQENSSNGLGSLLVR
                     DRENGHILERSSSWSAQTGTHHPDWARLSAWLDGQFPLHRDPLRLWTDCELNLR"
     gene            9800..10303
                     /locus_tag="VHV99_23280"
     CDS             9800..10303
                     /locus_tag="VHV99_23280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX3638346.1"
                     /translation="MQHLKYLSGYPSALQEKVQKLIADEQLGPYLAKRYPGTHGVQTD
                     RALYAYCAELKREHLRNGAQIDKVTYDSKLDVVRHALGLHTSISRVQGGKLKAKKEIR
                     VASLFKGAAPEFLKMIVVHELAHLKESDHNKAFYRLCEFMQPGYHQIEFDLRLYLTYR
                     DLTKSLS"
     gene            complement(10389..12707)
                     /locus_tag="VHV99_23285"
     CDS             complement(10389..12707)
                     /locus_tag="VHV99_23285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HEX3638347.1"
                     /translation="MKKPAARIEPYTHPAAGWGALKYVAINLIKEKVTGGDYRMLFRQ
                     NQPDGFDCPGCAWPDREHASTFEFCENGVKAVAAEATSKRVTPAFFKEHTVEELMAQS
                     DFELEQHGRLTDPLVYDAVRDRYVPIGWDEAFDLIAEHLKRLDNPDSAAFYTSGRASN
                     EAAFLYQLFVRMYGTNNFPDCSNMCHEATSRGLPHTVGIGKGTVTLDDFEHADTLLIF
                     GQNPATNHPRMLGELRECAKRGATIVSINPLKERGLERFASPQHPVEMITMGSTKISS
                     VFIRPKVGGDFALIKGVAKRVIELDDEALASGLERVLDVAFIAEHTVGFDAFADDLRA
                     ESWDTIVAESGVPFEDVLKLADIYVKGRAVISTWGMGLTQHKNSVPTVQILSNLMMMR
                     GNIGRRGAGLCPVRGHSNVQGDRTVGIEERPTEAFLDRLGEVYDFEPPREHGLDVVNT
                     IQAMLDGKVKVFIGLGGNFSIATPDTPRTWEAMRSCDLTVHITTKLNRSHLVHGRDAL
                     ILPTLGRTEIDLQNGVAQGVSVEDSMSMVHISYGMNKPASANLLSEIAIVARMAHATL
                     GSGKVDWLAQTADYALIRDGIAQVIEGFQDYNERLKNPGGFHLGVASRERIWKTPSGK
                     AQFLVHAIDVDTPIHQARAEHGERLMTLMTTRSHDQYNTTIYGLDDRYRGVYGQRRVL
                     FANKDDLAMLGFVAGQRVDITSVWADGIERHAAGFLLVEYDIPRGCLGAYYPETNPLV
                     PLSSVADGAGTPTSKSIPVLLKASAIVDAIAA"
     gene            12878..14311
                     /locus_tag="VHV99_23290"
     CDS             12878..14311
                     /locus_tag="VHV99_23290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX3638348.1"
                     /translation="MKLYEKLADELTEAVRRGVFAAGERIPSVRQASQQHGVSIKTVL
                     HAYALLESRGIVETRPQSGYFVRDAVATAVALDESRLSRQPAAQTPPVAAAVDVSRLV
                     LSTLRSIRAHDAVPLGSPYPDPSLFPWRRINQYANAIARRQASWNLIDDLPPGNPELI
                     RQIARRYAENGVPVDPDEIIITLGATEAINLSLQAVAKPGDTVAVESPTYYAMLHAIE
                     RLGMRAIEVATHPVHGIDIEALAQVIARQKIAACMVMPNFQNPLGFQMPDARKQQLVA
                     LLAAHEIPVIENDVYQELYFGEARPSTLKNFDTRGLVLHCASFSKSLTASYRIGWAMP
                     GRYRAQVEKLKFLNTLTTPSVPQVAVADYLKQDGYDRHLRHVRKVYRQQASIMTAMVQ
                     RFFPAGTRMSTPQGGYVLWVELPGRVDSMRLYQAALARSITIGPGMMFSMRDDFRHFI
                     RLNYSYPWTAQTEAALKTLGELVAHLT"
     gene            14342..14647
                     /locus_tag="VHV99_23295"
     CDS             14342..14647
                     /locus_tag="VHV99_23295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3638349.1"
                     /translation="MEDDELDPVLVAVLAQLWRAYQETPGGAWSLAKLSKQADVPMSG
                     LRRQLTALVDGGLVDTTFSEEGTGTARLSELGQGLCAQLFDESGPDAEQADPPPQVH"
     gene            14870..16012
                     /locus_tag="VHV99_23300"
     CDS             14870..16012
                     /locus_tag="VHV99_23300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX3638350.1"
                     /translation="MKTRLARALHHSLRIKSVRTVLAAAFGAALCTSLAFNAAAAAAA
                     SAQPLGIAFVYLGNPGDAGWTYAHEQGVKEVEAKFGDKIKVTRVENVPESADSERVFR
                     DLASKGNKIVVGSSFGFQDFELKVAKDFPDAVFEHATGYKKAANFATYDVRTYQSAYL
                     AGLVAGYTTKSNTLGFVGSVPVPEVVRNINAFTMGARSVNPKARVKVVWINSWFDPGR
                     EKQAAETLIGEGADVLIQNTDSTATMQTAEQKKVHAFGWDSDMKKFGPNAQLGACVSN
                     WGVYYTHLAQQVMAGTWNNSPVWWGLKEKAIDLADINTDAVSPAAQKALNQKRDDIIA
                     GKFNPFAGPIADQSGVVKVAAGKSLSDVELLRLNWFVQGVDGSLPK"
     gene            16056..16604
                     /locus_tag="VHV99_23305"
     CDS             16056..16604
                     /locus_tag="VHV99_23305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEX3638351.1"
                     /translation="MSLTVSTDAAAAGAAATYPPQGLTPTHEQIMRHLRHSSRVAERA
                     TLLGHHPFGAVLVGPDQETVLMEQGNVDTVNHAESVLARVAALNFTPEYLWSCTLYTS
                     VEPCCMCAGTMYWANIGRVVFGMTEKRLLEATGDHAENPTMSVDCRYVFDHCQKPVEV
                     IGPVAEVETEVMEVQRRFWSGR"
     gene            17028..17873
                     /locus_tag="VHV99_23310"
     CDS             17028..17873
                     /locus_tag="VHV99_23310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX3638352.1"
                     /translation="MKQHLVRWGCAAAGLLALPAVTFAQSQAYTSSTVNVRAGPASDY
                     PIVTQLPGGVPVTVMGCVSNYQWCDVAAPNLRGWVYAGRLSYPYQGSNVPVMSYGTVI
                     GLPIVTFSIGTYWGNYYRGRPWYGQQSRWAHHPPPPPPRPGAGRPPGGRPPGGPPPGN
                     AGGRPPGGPPPGNAGGRPPGGPPPGNAGGRPPGGPPPGNAGGRPPGGPPPGNAGGRPP
                     GNGGGHPAGGPPPGNAGGRPPGGNAGGRPPEGNAGGHPQGGGGRPSGEGNGGGGGGRP
                     PDQRG"
     gene            18319..20628
                     /locus_tag="VHV99_23315"
     CDS             18319..20628
                     /locus_tag="VHV99_23315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018435382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX3638353.1"
                     /translation="MTREATSDSTMGATPEGTHYGSNLAEGVLASERTVLRLITRNTP
                     LPELLNEVCRRAEALLGKGATCSILLLDADGVRARVGGAPSLLVHFSAAIDGVAIGPR
                     AGSCGTAMYERRMVAVEDIETDPLWADFRHLALPLGLRACWSVPFENDAGTVLGAFAV
                     YYRTSRRPTLEEEAMLHDISRSVGLAVHQDAMAQRLARSEEHHRLVVDHLNEGIVVQS
                     RDGVVLACNPSAQRILRANADLIGRDILSVMVRVYHEDGSPLTEAERPTTRVLATGKP
                     VLGTTVGLELTDGDVVWITENVVPIVKPGDSEPSSVLISFTDIGPVREAQRQLKFLAT
                     RDPLTGLYNRAYLAERMRDLFAPVGVTGMGELARVAVLFVDLDGFKKVNDTAGHEAGD
                     ALLCSVAERLSACVAPDDTLARVGGDEFVIVVSAYESTGHLIALAQRILEMIAVPFAV
                     ADNEYYMGASIGISLFPEDGQDVPTLMRNADSAMYHAKQCGRNNFQFFTAELNQHLQR
                     RFMIEQALRRALAANELSLVYQPIVDSQCGRTIGAEALLRWYNSELGNVSPVEFIPVA
                     EDAGLIGEIGGWVLARACEQIAQWRRTLAPDLIVAVNLSPRQFKGGLVERIERCLAQS
                     GLEPEALELEITERLLMSDSDAVLPMLSALNAMGVRISVDDFGTGYSSLSYLKRFPLH
                     NLKIDRSFVAGLPDHRDSIAITQAVVAMAHSLGMNVTAEGVETAEQAAFLRGIACDKQ
                     QGYFYSRPVGASAYARSLSDAQMGLARAS"
BASE COUNT         3920 a         6238 c         6779 g         3694 t
ORIGIN      
        1 gcaggcggcg gctgatctga acacgatcgc agcgcacacc agcggtacga tcaatgcaac
       61 ggcggccacg acgattaccg gcattgctac cgatgtggca gcagcggtga cggctggcga
      121 catcatcctg tcgggcaccg aagcggtaac cacgacggat gcgtcgcaag cggcggctga
      181 tctgaacacg atcgcaacgt acaccagcgg cgcagtgaac gcggcggcag cgagcacggt
      241 tacggggctg gtggcagacc tcgagacggt cgtggcttcg accaatatca gcctttcggg
      301 cagcgaaacg ctctcggtaa cggaccccac ggtgacgatg gccgaactga acgcgctcgc
      361 cgcgcttacc ctcggtaccg tcgatgcaga cgcggtgctc acggtcacgg ggtcggtgac
      421 agacatcgag acggccttgg cgtcgaatcg cattacgttc agcgccgccg aatcgttcac
      481 ggtacaggac accacggtgg cggcgtccga tctgaacacg atcgccgggt acaccagcgg
      541 gacggtggac gcaacggcag ccacgacgat tgccggttcg gccgcggatg tggcgactgc
      601 gctggccgcg attgacatca ggttgagcgg taccgagatc gtggcgatca gcgacaccgc
      661 ggcggcggcg gcagatctga aggcgatcgc tgcggacaca agcggtcagg tgacggcaac
      721 gtcggtgatc acgctgagcg gttcggccgc cgacgtagcg gctgtggtga tcgcgagcaa
      781 catctcgctg agcgatgccg agagcgtgac gatcagcggc accacggcgg cggcagccga
      841 tctgaacacg atcgtcaata acaccagcgg cacggtggac gcgatggcgc tgggcacgat
      901 cacgggatcg gcggccgacc tcgagacggc cctggcgtcg accagcatca ctttcgcggg
      961 caacgaaaat ctgacgctga gcgacacctc ggtagcagtg tccgtgttga gcacactcgc
     1021 cggcgaaacc accgggacgg tgaacgcaac ggcggctacc ctggtgacgg gtactgcggc
     1081 ggcggtggcg gcggcggtga tcgcggccga catcaccctg tcgggcagcg aggcggtgac
     1141 cacgtcggac gcgacgcagg cggcgggcga tctgaacacg atcgcggcga acaccagcgg
     1201 cacggtgaac ggcgccgcgg ctacgacgat taccggttcg gctgtggcgc tcaacgccct
     1261 gctgatctcc tccaatattg tcacttccgg taccgaaaac atcacggtgt cggatgcttc
     1321 ggtggcggcg tcggacctct ccgcgatagc cgggcacacc actggcaccg tcaatgcgga
     1381 ggcgaccacg ataaccggca gcgcgagcgc gatcattgcc gcgcacagca cgtccaacat
     1441 cagcatttcg agcagtgccg tctacacggt aaccggaacc gctacggtgt cgcaggtctc
     1501 gacgattacc ggctacacca ccggggcagt ggcctacagc atctacgatt cggtagccaa
     1561 cctgtccacc agcaacgcgc attacgctct gctgctggct gcggccacgg tcaccgctta
     1621 tctggacagc tcgcacaaca atctgacctt ggtatcgctc gataacggtg tcgggagcat
     1681 cgacctgaat ggccaggcgg ccacgctcac caacagtgag ctgagcctga acattgtcga
     1741 cagcgtcggt ggcgccacgg tgaaagaggt ggcgacgtct aacctcgacc tgtcggctgg
     1801 cgtggattcc tacgtgaact ccctcgatct ggccggccat accgtcaagc tcaccgcgtc
     1861 cgagatctcc ggattgacgc tggtcgactc ggttggcggc ggcacggtaa cggagaagct
     1921 ggttgccgat accaacctga ccggcggtgt ggacagccac gtgtccaata tcgacatggc
     1981 cggccatgtg gtcacgttga gcgcctctga attggccacg ctgtcattga gcgactcagt
     2041 gggtggcggc tacgtgatcg agcagctcca gggttcgacc aacctgacga ccggcctcaa
     2101 cagccatgtt caggagatcg atctcgccgg ttacacggtc aagctgacgg acacggaact
     2161 ggccagcggg ctggcccacg gtgtcatgga ctcgtcgcag ggccacaccg ggtcggtggt
     2221 ggagcagttc accacaagcg tcgacctgac gttgacgtcg agcacactca accccctcgt
     2281 gcacggactc gatctgaacg gcaattcggt gacgttgacc gatacgcagc tcggccagat
     2341 caacttcgtg aacgatacgc tgggcggtgg ctcggtgacg gaatccgata cgcatgcgct
     2401 gaacaacggc gacacgcttt cgctggtcac gcattcgacg acgaccctca acctcgacct
     2461 gaataacgag gtagcggcga acgccacggt gaccatcgcg ggcctgcagc acaacgacac
     2521 gctgaacgtc ggcgccggca gcaccctgac gtcagcggtg ggcagtgcgc agacagtcaa
     2581 ccaggcaggc gagtacttct tcgacaccag tgcgaagacg ctgacctggg acgactcgag
     2641 ttcgcatgtt cattcggttg tgctggccaa cgtgacgagc gtcacgctgg agccgaatca
     2701 gcacacgttc acggtgatct gaccgtgtcg aagcagcgcc gatgaagact gagtaagacc
     2761 gaatggcccc atcgaagtcc gatggggccg tttctcgatt gagctagaaa agcgttcgac
     2821 agacaacggg ggtcgaacgg ccgtaagaaa accagaagtt ggtgtacaaa atgttcttga
     2881 agaattacat ggcaggtgcg gtagcaaccg cgcttgttac ggcgagcctc ggggcctcgg
     2941 ccgccgattg gtcgctaggc cagatggcgc ggcaggcgct cgccacgcat ccggaaattg
     3001 tcggcaagca atattcgctc aatgcagcgg aagcgaatgt gaaggccgcg tcgtggcaac
     3061 gctttccgac cctcgccgtc gaaggcggca aggccaacga cggcaacccg cgctttaccg
     3121 atatcagcct gacgcagccg atctggtccg gcgggcgcat ctcggggacc atcgcctccg
     3181 ctgaggcgca aaaggacgcg gcctcggcga gcgtcgacga aacccgcgaa gatattctgc
     3241 tgcgggtggc gacggcttac gtcgaggcga tgcggcagca ggcgcgcacg gcgatcgccg
     3301 aaaaggaagt ggagcagcat cagcagctgt tcgacatgat cagccggcgc gtagacgccc
     3361 aggcgagcgc cacagtggac cgggacctgg cgcagtcccg cctgtaccag gcggacaacg
     3421 atttatcgac ggcaaagcag gcgcttgcca cagcgctctc acagctctcg cagctcgtcg
     3481 ggcagcgcgt gccggccgtc gacgactaca gcatcgacca gatgatgccc gattccgtcg
     3541 ataccgcggt cgacctggcc accggcgtgt cgccgacgct caagcgatac gattatcagg
     3601 aaaaggccgc cgaagccgat atcacagtga aaaaggcggc ttacctgccg caggtttcgg
     3661 tgcgcttcga caagttctac gggcaggatc tgattacgcc gcaagacacc acgcgtggaa
     3721 gccgcgtcat gctcgttatc caggcggaac cgggcgcggg cctgtcggcc gctgccggcg
     3781 tcggcgcggc tgtcgccgcc aagaacgccg ccatcgaagc acgcaccgcc gcgctgcgcg
     3841 acctgcggct gttggtgtcg accgactggg acgagtatat gggcgcccgc gagcgcttcc
     3901 agagctcgac cttgtccacc gccagttcga aggcggtgta cgagtcgtac gtgcgccagt
     3961 acacgatcgg caagaagtca tggctcgacg tgatgaatac cgtgcgggaa gcgtcccagt
     4021 ccgaaatgac gactgcggac gccaaggcgc agatgaccgc cgcggcgttg cggctcgaag
     4081 tcaggaccgg cgagacgccg ttcctcgacg aagccagcaa cgtccccccc gctcaaaaca
     4141 attcgacccc atgaacccgc tcctctccgc aattacgctg gctgccaagg cagtcggcca
     4201 gaccgtcccg caagcgctgc tgcaagatgt ccagactgtg ctcgaggtgg gcgccgatgc
     4261 gccggtgctc gagcgggtca gggcagcctg gcaggccggc agtctcaccg gcgaagtcta
     4321 cgaactcgcg gcgccaggcg ccggacaagg cccgtttctc gcgcgtgccg cgtcgggcga
     4381 atggtggatc gtgctggccc gcaacgccga cggcagttgg accgcacgcg cggccgacgg
     4441 ccagattgcg cggctcgacg gcctcgaggg cgccgtgtgc atcggcctgc ctgtcgagca
     4501 cgacgatgcg ttcggcggct ccgatccagc cgatccctcc agcccaaaca cgtccgccaa
     4561 gcgtctcgtg ctgcaggcat tgtggcgcaa caagtcgcac tacatcgaag cgatcctggc
     4621 gacggtgctg accaacatcc tcgcgatcac gacctcgctc ttcagcatgc aggtgtatga
     4681 ccgggtgatc ccgaaccgcg gcttcgccac actcgtggtg ctgtcggtcg gcgtcggcgc
     4741 ggcgatcctg ctggagttgc tgctcaaaca tgtgcgcggc gcgtcgatcg agcgcacggc
     4801 caggcgcatc gacgagagtc tgtcggtatg gttttataac cgcctgatca acatacggct
     4861 tgaacaccgg ccgtcgtcga tcggctcgct cgcctcgcag gtcaagggct tcgagtcggt
     4921 gcgcgcgatc ctgagttcga cctcgttgtt cgtgctggtc gatattccgt tcgcattgct
     4981 gtttatcctg atcatcgggc tgatcggcgg ccggctcgtg ctggtgccgc tgctgatgtt
     5041 cccgatctcg ctgtgcgcgg gcttgatgtt ccagagacag attgccttcc attcgcggca
     5101 gaaccagggg cagagccacc gcaaggccgg tttgctggtg gagtcgatcg acggcgcgga
     5161 atcgttcaag gccaacggcg ccgactggtc gctgcagcgc cgctggtcgg gactcgtcga
     5221 cgatgccggc gcgtctgact acaaggtgaa aaactattcg tcgctgtcgc agcacttgac
     5281 tgtggcgctg cagcagctcg gctatgtcgg gctggttgca ttcggcgcat atctggtcgc
     5341 cgataacaag ttgaccatgg gcgcgctgat tgcctgcacc attatcagcg ggcgggccat
     5401 gtcgccgatc gcgcaattgc ccgcgacgct ggtgcagtgg tccaatgcgc gcgccgcggc
     5461 gaacggactc gacaagctga tctgcctgcc caacgacaac gacgcgcgcg gccatgcact
     5521 ggttccggaa atcgtcgacg gcgcgatgcg catcgacggc gtcaagttca gttatggcca
     5581 tggtggcgac gcggccgtcg atatcgagca gctcgacatc aagaccggcg agcggatcgg
     5641 catcatcggc tcgatcggct caggcaagag cacgctgctg aaactgatgt cgggcctgtt
     5701 ccggcccgcc gctggccgga tctcgatcaa cggcatcgac atggcgctga tcctgcctga
     5761 ggtcctgcat gaaaagatcg cctacctgcc gcaggaaatc cgcctgatca gcggcacctt
     5821 gcgcggcaac ctcgtgcaag gcctgcccga tcccggcgac gagcgtctgc tcgaagtcgc
     5881 caggctgacc ggattgatgg atctgatcac ccggcatccg aagggactcg cactgccgat
     5941 caccgaaggc gggcgcggta tttccggcgg acagcggcaa ttgatcgggc tcacacggct
     6001 gttgctgatg cggcccaaag tgctggtgct cgacgagccg accgcatcga tggactcggc
     6061 caccgagaaa cgcatcgttg ccgtgatcga cgcgatcgcc gcgcgcggca caaccgtgct
     6121 ggtcgccacc cacaagaccg cgctgctgcc ggtgacccgg cggctgctgg tgatgcgcga
     6181 cggccgcgtt gtgatggacg gcccgcgcga tcaggtcatc gacaaactgt tcggcacgtc
     6241 gccggttgct tctacctctg aggtttgatt cgctttatgt cggacattac acaagaaatt
     6301 ccgcgtgccc ggtgggcgct gaccgcatgc tgcggggtgg cgatagtcgg tttcatcgtg
     6361 tgggcgcgct gggcttcgct cgatcagatc acgcgggcac aagggcaagt gatcgccagt
     6421 tcgcgcgacc agatcatcca gtcccaggat ctgatgacca tcgacgccgt tctggtcaag
     6481 gaaggtgcgg acgtcaagcg cggccagccg ctggtgcgct tcgagcgcac tcgcgccgag
     6541 gcagcctatc tcgaatcgca gagcaaggag atgtcgctcg aggcggaggt agcgcgtctg
     6601 cacgcggaag tttacggcgg cacgccgcag tttcccaaat cactcgacgc ctattccgat
     6661 atccgctcga acgaggaact gctgttccgc aaacggcaag cggcggtcga cgacgaggtc
     6721 gcggcgatgc gcaagtcgct ggaatacgtg caggacgaac tcaacatcaa cctgccgctg
     6781 ctggcatcgg gcgatgtcag cagggccgac gtgctcaagc tcgagcggca ggtcgccgat
     6841 ctccaggggc agattaccaa ccggcgcaac aagtacatgc aggattcgca gaccgatctg
     6901 tccaaggcgc ttgaggatct cgcgggcgtt gaccaggtcg ttgcgcagcg caaggaccag
     6961 ctcgaccaca cggtgatcga ttcgccggtc gacggcattg tgcgcaacgt ccgcattagc
     7021 acgcccggtg gcgtggcgcg cgccggcgat gaaatcatgc agatcgtgcc ggtcgacgac
     7081 gagttgatca tcgaggctaa ggtcaaaccg aaggacatcg ccttcatcaa gccagggctg
     7141 ccggcgaaga tcaagctgga tgcgtacgac tacacgatct acggcacgct gtcgggcacc
     7201 gtgacgtaca tcagtgcgga tacgctcaat gacgacaccc atcctgccaa tgagcagccc
     7261 ttctaccgcg tgcaggtgaa gacaaacggc aatgatctgg ggcgcgcggt ggggcatcgg
     7321 atttccgtgc agccgggcat gacggccacc gtggaaatca agaccggctc gaacacggtg
     7381 ctgaactacc tgaccaagcc gatcaccaag accgtgaccg aatcgatggg cgagcgctag
     7441 tgcgtgtcag gagggcgcag cgatgcgccc gtcaccgtgc tcgctcgaac cggtccgact
     7501 ttacgtccgg cgaaccggct tgaccgcgct tcgccggtct tgcgcggtga gcgtgcgaaa
     7561 atccagcggc gtcatcccat aggcccggcg gaaggcgcgc gtgaaatcgg tggcgctctt
     7621 aaaaccgagt tcgtagccga tatttgccac cgcggtctgt ggcttgcgga tcaggtcatc
     7681 ggctgcatga cgtagcctga tgtgacggat gtaggcgccc acaccgccct catgctgaaa
     7741 caggcggtac agggtctggc gaggaagtcc caacgcgctc atcacgcttt cgggcgagag
     7801 tgccgcgttg tgccggttgg cctgtatata tcgtctcgcc tggccgaaca tggccgcgcg
     7861 gccggctgcg cgcgcattgc cgctcaggag tgcctgtttg ccaaacagtg cgatcagcat
     7921 ctgcgcgccc gagcggatgg cttcgtccgc tgactcggcg ctcatctgtg cgatgttctg
     7981 actgagcgcg ctcagatggt cgacgactaa ccgcgtgagc ggtgtcgtcc cgtggatcgc
     8041 gcggccatgg atggcctccg catcaggaaa ttccgcctgc accgtctcgc tcggcacgaa
     8101 cagcgtgagc accctgcacg cgttgcgctg catcctgacc ggttggttca aatcgagcgc
     8161 caggatcgtt gccgcgggcg caggcgcgct tttggggggc gtggcgcgca gtgtgatgtt
     8221 ctcaacgctg ccctccagaa acacgtgaaa agcgacgtca cgcaccttgt cggtcgaaat
     8281 tcgggcctgg gagcggtcga gcatcatcct gtcggaccgg caatcggtaa acgcaagttg
     8341 gccaatctga tagcgatcga tcgaagcctg gaacggttcg tcgagctggg atagcgaggg
     8401 cagcacgtcg atcacatggc cgacccgatc ccgccaggct agcaactgcc gctggggcgg
     8461 tttcgtacgc acgtcgaaat gactatgggc aatgccgggt ttgagcggag aatgaacttg
     8521 aattggattc gcgccgaggt tcatgcgggc gctcctttac ggaaataggc aaagaggggt
     8581 ggatcgaaca gtgtccgaat ttaaccagaa acgtgtcgga atgtaaccgg aaaatgctct
     8641 tgacgaggca ctatttaaaa gaagatgtgc tcgaaaccgc taacaaaaag tctggcacgt
     8701 gggcgcgagt gacaaaagtg gccagccttc ttcagagggc tgccttgagg aagtcgagcc
     8761 gttgattcta aaaacgtaaa gttatcctat ggaaaacgtt tgcgaggcgc gttttattgg
     8821 gggtttgctg aagtggatag cgggctgagc aatataagga gaggccctaa gcgctgacct
     8881 tttccggctt tacttgccac ccattgactg gcgtgggcct ctcacgccga aacggcaatc
     8941 tcacgctaat tgcccgacga gacatgccgg cagaacgcgc cacgtcgcgc ggtccgttcg
     9001 ctcgatctga ccgtccgtta caatcaacca tgaagtccag tcgtggctag catgcgcgac
     9061 gagccggatc tgcagcgcgt tgttggaacg cagccggtct aatcacgttg ggtgttcatg
     9121 cgagcgccat ggagccggag aggccaatga agaacggaaa gccgaagttc acgcgtcagc
     9181 atgctgacgg attgttccct gatttgaaac cgagcggcgc ccacatcgga agtcggcccg
     9241 acaatccgtt tggcggcgaa gcgttcgagg cagtcgaccg gcatgaccgt gacgaaaaat
     9301 ctctctggaa agagaacctc gtcaccttgc ccatggggat cgagttgccg cccgggtatg
     9361 agtcggcctc ccgtgtgcgc gaagcgatcg cgcgggcgtg gcggatgaaa tgggtccggg
     9421 aagcgaggaa cgagatcgtt gcggaattcc ccgaaggctg gtcggccgtc aggccaaaaa
     9481 gcgggccggt ggagttacgg gacgcgaacg gggttgtccg cgctctatac ggttgggcag
     9541 aagatgccga actcaggatt ctgccgcgct atctggtgga atcgcaggag aacagctcga
     9601 acggtctcgg cagtttgctt gtacgtgatc gcgagaacgg acacattctt gagcgatcgt
     9661 cgagctggtc tgcgcagacg ggtacccatc atccggattg ggcgaggctg tctgcgtggc
     9721 ttgacgggca gtttccgctg catcgggatc cgttgcgcct atggacggat tgcgagctca
     9781 acttgcgtta aataccgcca tgcaacatct gaaatacctg agcggctatc cgtccgcgct
     9841 acaggaaaaa gtccagaagc tgattgctga cgagcaactg ggcccgtatc tggcgaagcg
     9901 ctaccccggc acacatggcg tgcaaacaga ccgggccctg tacgcgtact gcgcggagct
     9961 caagcgcgaa catcttcgca acggcgcgca aatcgacaaa gtcacttacg acagcaagct
    10021 cgatgtcgtg cggcatgcgc ttggcctgca cacgagcatc tcgcgcgtgc agggcggcaa
    10081 gctgaaggcg aaaaaagaaa tccgcgtggc gtcgctattc aaaggagcgg cgcccgaatt
    10141 cctgaaaatg atcgtcgtgc atgaactcgc tcatttgaaa gaaagcgacc ataacaaggc
    10201 gttctaccgc ctctgcgagt tcatgcaacc gggctaccat cagatcgaat tcgatctgcg
    10261 tttgtatctt acgtatcgcg atctgacaaa gagcctgagc tgagcttcgt tcaggcctca
    10321 ggcttcaggt gtcgcgcgcc gggcggcttt cgccacccgg aacacagtgc ccctgaaacc
    10381 accatccctc aggcagcaat cgcgtcaacg atcgccgacg ctttcaacaa caccggaatc
    10441 gacttcgaag tcggcgtccc cgcgccatcg gcaaccgaag aaagcggcac cagcggattc
    10501 gtctccggat agtaagcgcc aagacacccg cgcggaatgt cgtactccac cagcaggaac
    10561 ccggccgcgt gacgttcgat tccatcggcc cacacgctcg tgatatccac gcgctggccc
    10621 gcaacgaatc ccagcatcgc cagatcgtcc ttgttggcga acagcacgcg ccgctgcccg
    10681 tacacgccgc ggtaacggtc gtcgaggccg tagatcgtcg tgttgtactg gtcgtgcgaa
    10741 cgcgtcgtca tcagggtcat caggcgctcg ccgtgctctg cgcgcgcttg gtgaatcggc
    10801 gtgtcgacgt caatcgcatg cacgaggaac tgcgccttgc cgctcggcgt tttccagatc
    10861 cgctcgcgcg atgccacacc caggtggaag ccgcccggat tcttcagccg ttcgttgtag
    10921 tcctggaagc cttcgatcac ttgcgcgatg ccgtcgcgaa tcagtgcgta gtcggctgtc
    10981 tgagcgagcc agtcgacctt gccgctgccg agcgtggcgt gtgccatgcg cgcgacgatc
    11041 gcaatctccg acaacagatt cgccgaggcc ggcttgttca tgccatacga gatgtgcacc
    11101 atgctcatcg aatcctcgac gctcacgcct tgcgccacgc cattttgcaa gtcgatctcg
    11161 gtgcgcccga gcgtcggcag aatcagcgcg tcgcggccat gcacgaggtg gctgcggttc
    11221 agcttggtgg tgatgtgcac ggtcaagtcg caggagcgca tcgcttccca cgtgcgcggc
    11281 gtgtccggcg tggcgatcga gaagttgccg ccgaggccga tgaacacctt caccttgccg
    11341 tcgagcatcg cctggatcgt gttgacgacg tcgaggccat gctcgcgcgg cggctcgaaa
    11401 tcgtagactt cgccgagccg gtccagaaac gcctcggtcg gcctttcctc gatgccgacc
    11461 gtgcggtcgc cctgcacgtt cgagtgtccg cgcaccgggc acaagcccgc gccgcggcgg
    11521 ccgatattgc cgcgcatcat catcaggttc gacaggatct gcaccgtcgg cacggaattc
    11581 ttgtgctgcg tgaggcccat gccccacgtc gaaatcaccg cgcggccctt cacgtagatg
    11641 tcggcgagct tcaacacgtc ttcaaacggc acacccgatt cggcgacgat ggtatcccag
    11701 ctttccgcac gcaaatcgtc ggcgaacgcg tcgaagccga ccgtgtgttc ggcgatgaac
    11761 gcgacgtcga gcacgcgttc gaggcccgag gccagcgctt cgtcgtcgag ttcgatcacg
    11821 cgtttggcga cgcccttgat cagcgcgaaa tcgccgccga ctttcggccg gatgaacacg
    11881 gaactgatct tggtgctgcc catcgtgatc atctcgaccg gatgttgcgg gcttgcgaag
    11941 cgctcgaggc cacgttcctt cagcggattg atcgacacaa tggtcgcgcc gcgtttcgcg
    12001 cactcacgca gctcgccgag catgcgcgga tggttggtcg ccggattctg gccgaagatc
    12061 aggagcgtat cggcatgttc gaaatcgtcg agcgtgaccg tgcccttgcc gatgccgacc
    12121 gtgtgcggca agccgcggct ggtcgcttcg tggcacatgt tcgagcaatc ggggaaattg
    12181 ttggtgccgt acatgcgcac gaacaactgg tacaggaagg cggcttcgtt gctcgcgcgg
    12241 cccgaggtgt agaaggcggc gctgtcgggg ttgtcgaggc gcttgaggtg ttcggcgatc
    12301 aggtcgaagg cttcgtccca gccgatcggc acgtaacggt cgcgcacggc gtcgtagacg
    12361 agcgggtcgg tcagacggcc gtgttgttcc aattcgaagt ccgactgcgc catcagttct
    12421 tcaaccgtgt gttccttgaa gaacgccggc gtcacgcgct tgctggtcgc ctcggcggcc
    12481 acggccttga cgccgttttc gcagaactcg aaggtcgacg catgctcgcg atccggccat
    12541 gcgcaaccgg ggcagtcgaa gccgtctggc tgattctgcc tgaacagcat gcggtagtcg
    12601 ccgcccgtca ccttttcttt gatcaggttg atggcgacgt atttgagcgc gccccagccg
    12661 gcggcggggt gggtatacgg ttcaatgcga gctgcgggtt tcttcatgat cttgcctccg
    12721 gatgaggcct ggacgaggct ttagtgccga cgtgcagaag actactgtgt tccaaaagcg
    12781 gtgcagcata cagaaaatcg gaaagctgag ggatacagtt ctttgatatt tgtcatagac
    12841 tgacgtttcc gcccacacgt ttgtttgagc cacgtccttg aaactgtatg aaaaactcgc
    12901 cgatgaattg accgaagccg tgcgccgcgg cgtcttcgct gcgggcgagc ggattccatc
    12961 ggtgcgccag gcaagccagc agcacggcgt cagtatcaag accgtgctgc acgcatacgc
    13021 gttgctggaa agccggggga tcgtcgagac ccgtccgcag tcgggctatt tcgtgcgcga
    13081 cgcggtggcg acggcggtgg cgctcgacga gtcgcgtcta agccgccagc cggccgccca
    13141 aacgccgccg gtggccgccg ccgtcgacgt gagccggctg gtgctgtcga ccctgcgcag
    13201 catccgcgcg cacgacgccg tgccgctcgg ctcaccgtac cccgatccgt cgctgttccc
    13261 gtggcgccgc atcaaccagt acgcgaacgc gattgcccgc cgccaggcga gctggaacct
    13321 gatcgacgat ttgccgcccg gcaatcccga gctgatccgg cagatcgcgc ggcgctatgc
    13381 cgagaacggt gtgccggtcg atccggacga aatcatcatc acgctcggcg cgaccgaggc
    13441 gatcaacctc agtttgcagg cggtcgccaa gccgggcgat acggtcgccg tcgagtcgcc
    13501 gacttactac gcgatgttgc acgccatcga gcgtctcggt atgcgggcaa tcgaagtggc
    13561 cacgcatccg gtccacggca tcgatatcga ggcgttggcc caggtcatcg ccaggcaaaa
    13621 gatcgccgcg tgcatggtga tgccgaactt ccagaatccg ctcggtttcc agatgccgga
    13681 cgcgcgcaag cagcaactgg tcgcactgct cgccgcgcac gagattcctg tcatcgagaa
    13741 cgatgtctat caggagttgt acttcggcga ggcgcggccg tcgacgttga agaacttcga
    13801 taccaggggg ctggtgctgc attgcgcgtc gttttcgaag agcctgacgg cctcgtatcg
    13861 gatcggttgg gccatgccgg ggcgctaccg cgcgcaggtc gagaagctga agtttctcaa
    13921 tacgctgacc acgccctcgg tgccgcaagt cgccgtcgcc gactacctga aacaggatgg
    13981 ctacgaccgc catctgcggc acgtgcgcaa ggtgtaccgg cagcaggcaa gcatcatgac
    14041 agcgatggtg cagcggtttt tcccggcggg cacgcgcatg tcgacgccgc aaggcgggta
    14101 tgtgctgtgg gtagaattgc ccggacgcgt cgattcaatg cggctctatc aggcggcact
    14161 cgcgcgcagc atcacgatcg ggccgggcat gatgttttcg atgcgcgacg attttcgcca
    14221 tttcatccgg ctcaattaca gctatccgtg gacggcgcag accgaggcgg ctttgaagac
    14281 actgggcgaa ctggtcgcgc acctgacttg acaggtttgg agaggcaagg agacatacac
    14341 gatggaagac gacgaactcg atccggtgct ggtggccgtg ctggcacaac tgtggcgtgc
    14401 gtatcaggaa acgcctggcg gcgcgtggtc gctcgccaag ctttccaagc aggcggacgt
    14461 gccgatgagc ggcttgcggc ggcagttgac ggcgctggtc gacggtggtc tggtggacac
    14521 caccttcagc gaggaaggca cgggaacggc gcgcttgagc gagctcggcc agggcttgtg
    14581 cgcgcagctg ttcgatgaga gcgggcctga cgctgagcag gccgacccgc caccacaggt
    14641 tcattgagtg gcaacggaaa ttgacgtgtg aagctttgcc gatgtcctcg ttcgacgatg
    14701 tcgagcatat tttccgcgcg caaagataaa gatcccgata tgagcgggat aacgaataat
    14761 aatttttccg ctgaaattgg ccttcgaata tggcccccta tgctgaagcc tcgcccggga
    14821 gtgtcccggg cgctgtggca ggcacctgga aaccatcgtc ggagaattca tgaaaacaag
    14881 gctggcgcgc gcactgcatc actcattgcg gatcaaatct gtgcggacgg ttctcgccgc
    14941 ggcgttcggc gcagcacttt gcacatcgct cgcgttcaac gccgcggcgg ccgcagcggc
    15001 gagcgcgcaa ccgctcggca tcgccttcgt ctacctcggt aatccgggcg acgccggctg
    15061 gacctatgcg catgagcagg gcgtgaaaga ggtcgaggcg aaattcggcg acaagatcaa
    15121 agtgacgcgc gtcgagaacg tgccggagtc ggcggattcg gagcgggtgt ttcgcgacct
    15181 ggcgtccaag ggcaacaaga tcgtggtggg ctcgagcttc ggcttccagg acttcgagct
    15241 gaaagtcgcc aaggactttc cggacgccgt gttcgaacat gcgaccggct acaagaaggc
    15301 cgccaacttc gccacctatg acgtgcggac ctatcagagc gcctacctgg ccggcctggt
    15361 cgccggctat acgacaaagt ccaacacgct cggcttcgtc ggctcggtgc cggtgccgga
    15421 ggtggtgcgc aacatcaacg cgttcacgat gggggcgcgc tcggtcaacc cgaaggcgcg
    15481 cgtgaaggtc gtgtggatca acagctggtt cgatccgggc cgcgaaaagc aggccgccga
    15541 gacgctgatc ggcgaaggtg ccgacgtgtt gatccagaac accgattcga cggcgacgat
    15601 gcagacggcc gaacagaaga aagtgcatgc attcggctgg gactccgaca tgaagaagtt
    15661 cgggccgaac gcgcaactgg gcgcctgcgt gagcaactgg ggcgtgtact acacgcatct
    15721 ggcgcagcag gtgatggcgg gtacgtggaa caattcgccg gtgtggtggg ggctgaaaga
    15781 gaaggcgatc gatctggccg acatcaatac ggacgcggtg tcgccggcgg cgcaaaaggc
    15841 gctgaaccag aagcgtgacg atatcatcgc cggcaagttc aatccgtttg ccggcccgat
    15901 cgcggatcag tccggcgtgg tgaaggtcgc ggcgggcaaa tccttgagcg atgtggaact
    15961 gctgcgtttg aactggttcg tgcaaggcgt ggatgggtcg ctgccgaagt aatcaattga
    16021 ttggtttgcc taactattgc caaaggagat cgcctgtgag tcttaccgtc tcgaccgatg
    16081 ccgcagccgc tggcgcggcg gcgacttatc cgccgcaggg tttaacgccg acgcatgagc
    16141 agatcatgcg gcatctgcgg cattcgagcc gggtggccga gcgggcgacg ctgctgggcc
    16201 accacccgtt cggcgcggtg ctggtcggcc cggatcagga gaccgtcctg atggagcagg
    16261 gcaatgtgga cacggtgaat cacgccgagt cggtgctggc tcgcgtggcg gcgttgaatt
    16321 tcacgccgga gtacttatgg agttgtacct tgtacacatc ggtcgagcct tgctgcatgt
    16381 gtgccgggac gatgtattgg gccaatatcg gccgcgtggt gtttggcatg accgagaagc
    16441 gcttgctgga ggcgaccggc gatcatgccg agaatccgac catgagcgtg gattgccggt
    16501 atgtgttcga tcattgccag aagcccgtcg aggtgatcgg tccggtggcc gaggtggaga
    16561 ccgaggtgat ggaggttcag cggcgattct ggagcggcag atagcatgtt tgctcctcgg
    16621 cgctgctgag gtctgtttgc ctgcagcggc caggaatgaa aattaaagcc gggcgccgcg
    16681 cgggcaaaca caacgacgca aaaatctcac ggtggcgagt cagcctccac ggggtgcgag
    16741 catagccggt ccgcagagcc cgtcggcgcc gtgtggcgga tatttccact gaccgtgctt
    16801 ccctttgagc acaggagggc aacggtcatt cacaatgatc cccggattcg cgtttaccgg
    16861 acgctttcag gcatgagtat tgcaggcgcg gatggcaggc atgctttggc cgctacgtca
    16921 gcaggggcga tcacaggccc gacgcattca cacatgcgtg cagacaggca cgtgtgcggg
    16981 ccgtagcggc gcgcacgcaa gtgcgggacg taaaaacggg agaaaaaatg aaacaacacc
    17041 tcgttcgatg gggatgcgcg gcggcaggtt tgctggcgtt gcccgcagtg acgtttgcgc
    17101 agtcgcaggc ttatacgagc agcacggtca acgtgcgcgc cgggcccgct tcggattacc
    17161 cgatcgtgac gcagttgccg ggcggtgttc cggttaccgt gatgggttgc gtcagcaact
    17221 accaatggtg tgacgtcgcc gcgccgaacc tgcgcggttg ggtttatgcg gggcggctca
    17281 gctatcccta tcagggcagc aatgtgccgg tgatgagtta cggcaccgtg atcggactac
    17341 cgatcgtgac gttctcgatc ggtacttact ggggcaatta ttatcgaggc cggccgtggt
    17401 atggccagca atcccgctgg gcgcatcatc cgcctcctcc gccgccgaga ccgggtgcag
    17461 gacggccgcc aggcggacga ccgccgggcg gtccgccgcc ggggaatgcg ggcggaaggc
    17521 caccgggcgg tccgccaccg gggaatgcgg gcggaaggcc accgggcggt ccgccgccgg
    17581 ggaatgcggg cggacggcca ccgggcggtc cgccgccggg gaatgcgggt ggacggccac
    17641 cgggcggtcc gccgccgggg aatgcgggtg gacggccgcc ggggaacgga ggcggtcatc
    17701 cggccggcgg tccgccaccg ggcaacgcag gtggacgccc gccgggcggc aatgcaggtg
    17761 gccgtccgcc ggaaggcaac gctggtggtc acccgcaagg tggtggcggc aggccctcag
    17821 gcgaaggaaa cggcggtggc ggcggtggcc ggccaccgga tcagcgcggc tgatcgaaca
    17881 acgggcccaa gcggccagga gaagtgatcc tcgacccgct tggtcccgaa acgtgttccg
    17941 tctcgatgcg acacgcgagc ggaacccgtc cgatgcgtca cccattcgct ttagccgctc
    18001 aaccgatctc ggcaaaccga agtgcgccgt gacagtcttg gggcgttttt agcccgaagt
    18061 gagggcgaac ggcgcgtcta aacccacgcc gcccgaggcc cgcgcggcgc taatcgctca
    18121 cttggcgccc ctcgcgccgg cgggggcatg atcctcggat tcgccgtgtt gatgatgctg
    18181 tcgctgcatt ggttgaccgg ctgaccggcc ggtttatgca gttcgcgtca tgtatcgcga
    18241 ttaaagtgcg cgaccctcgc gtaaaccccg gtacgccgcg tacactacct tgccgatgtc
    18301 gggttagggg gcgcacaaat gacacgcgaa gcgacatcag atagcacgat gggagcaacg
    18361 cctgagggca cccactatgg gtcgaatctg gccgaggggg tgttggcgtc ggagcgtacg
    18421 gtgttgcggc tgattacccg caacacgccg ctgccggaat tgctgaatga agtatgccgt
    18481 cgcgcggaag cgctgctcgg caaaggggca acctgttcga tcctgttact ggatgcggac
    18541 ggcgttcgcg cgcgcgtggg cggcgcgcct tcgctgctgg tgcatttcag tgccgcgatc
    18601 gacggcgtcg cgatcggtcc acgggccggg tcctgcggca ccgcgatgta cgaacggcgc
    18661 atggtcgcgg tcgaagatat cgaaaccgat ccgctgtggg ccgatttccg gcacctcgca
    18721 ttgccgctcg gcttgcgcgc gtgctggtcg gtgccgtttg aaaacgacgc cggcacggtg
    18781 ctgggcgcgt tcgcggtcta ttaccgcacg tcgcgccggc cgaccctcga agaagaggcg
    18841 atgctccacg acatcagccg cagtgtcggt ctcgccgttc atcaggacgc catggcgcaa
    18901 cgcctcgcgc gcagcgagga gcatcatcgg ctggtcgtcg atcatctcaa cgagggcatc
    18961 gtcgtgcaat cgcgcgacgg cgtggtgctg gcctgcaatc caagtgcgca acgtattttg
    19021 cgggcgaacg ccgacctgat cggccgcgac attctgtcgg tgatggtacg ggtgtatcac
    19081 gaagacggtt cccccttaac cgaggccgag cgcccgacca cccgggtgct ggcgaccggt
    19141 aaacccgtgc tcggcaccac ggtcggcctg gagctgactg acggcgatgt cgtctggatt
    19201 actgagaatg tcgtgccgat cgtcaagccg ggcgacagcg aacccagctc ggtgctgatc
    19261 tcgtttaccg acatcgggcc ggtgcgcgag gcacaaaggc aactcaagtt tctggctacc
    19321 cgcgatccgc tcaccggcct ctacaaccgc gcctatctgg ccgagcgaat gcgcgatctg
    19381 ttcgcaccgg tcggcgtgac gggaatgggc gagctggcgc gggtcgccgt gctgttcgtc
    19441 gatctcgacg gtttcaagaa ggtcaacgac accgccggtc acgaggccgg cgatgcgctt
    19501 ctgtgcagcg tcgcggagcg gctctcggcg tgtgtcgcgc cagacgacac gctcgcgcgc
    19561 gtgggcggcg acgagttcgt gatcgtggtc agcgcgtacg aaagcacggg tcatctgatc
    19621 gcgctcgccc aacgcattct cgagatgatc gcggtgccgt tcgcggtggc ggacaacgag
    19681 tactacatgg gggcatcgat cggcatcagc ctgtttccgg aggacggcca ggacgtgccc
    19741 acgctgatgc gcaacgccga ctcggcgatg taccacgcga aacaatgcgg ccgcaacaac
    19801 ttccagttct tcaccgctga gctgaatcag catctgcaac gccgcttcat gatcgagcag
    19861 gccttgcggc gcgcgctcgc cgccaacgag ctgagccttg tgtatcagcc gatcgtcgac
    19921 agccagtgcg gccgcacgat cggcgccgaa gcgctgctgc gctggtacaa cagcgagctg
    19981 ggcaacgtct cgccggtgga attcatcccg gtggccgaag acgccggcct gatcggcgaa
    20041 atcggcggct gggtgctggc gcgggcttgc gagcagatcg cgcagtggcg gcgcacgctg
    20101 gcgccggatc tgatcgtcgc agtgaatctg tcgccgcgcc agttcaaagg cgggctggtg
    20161 gagcggatcg agcgctgtct cgcgcagtcg gggctcgagc cggaggcgct ggaactggag
    20221 atcaccgagc ggctgttgat gagcgacagc gacgccgtgt tgccgatgct aagcgcgttg
    20281 aacgcaatgg gcgtgcggat ttccgtcgac gatttcggca cggggtattc gtcgctgtcg
    20341 tatttgaagc ggtttccgct gcataacctg aaaatcgacc ggtccttcgt cgcgggcttg
    20401 ccggatcatc gcgattcgat cgcgatcacc caggcggtgg tggcgatggc ccattcgctc
    20461 ggcatgaatg tcactgctga aggtgtggag actgccgagc aggcggcgtt tttgcgcggg
    20521 atcgcttgcg acaagcagca ggggtatttt tatagccggc ctgttggggc gagcgcttat
    20581 gcgcgcagtc tgagcgacgc gcagatgggg ctggcacggg cgtcatgact g
//
LOCUS       DATASH010000872         2450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2916520,
            whole genome shotgun sequence.
ACCESSION   DATASH010000872 DATASH010000000
VERSION     DATASH010000872.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2916520"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            346..2193
                     /gene="recQ"
                     /locus_tag="VHV99_23320"
     CDS             346..2193
                     /gene="recQ"
                     /locus_tag="VHV99_23320"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEX3638354.1"
                     /translation="MSRPLEILNEVFGYPAFRGQQGEIVEHVASGGDCLVLMPTGGGK
                     SLCYQIPSLVRREAGCGAGIVVSPLIALMQDQVAALTEVGVRAAYLNSTLSSAEAMAT
                     ERALREGEIDLLYVAPERLMTPRFQELLERTRIGLFAIDEAHCVSQWGHDFRPEYIQL
                     SVLHERFPNVPRIALTATADAITRDEIIHRLALDDARIFVSSFDRPNIRYRIVEKDNA
                     RTQLLDFIRAEHSKPDGTTDAGVVYCLSRRKVEETAEWLKEKGMRALPYHAGMEFEIR
                     QKHQEMFQREEGIVMCATIAFGMGIDKPDVRFVAHLDLPKSVEGYYQETGRAGRDGMP
                     ANAWMAYGLGDVVQQRKMIDESDADDAHKRVQTGKLDALLGLCEAATCRRVRLLAYFG
                     ESSKPCGNCDNCIEPPDTWDATREAQMALSCVFRAQRASGFHFGAGHLIDILRGNRSE
                     KILQRGHEKLTTFGIGAALGEPEWRAIFRQLVAFGYLTVDHEGFGSLVLTEAAKAVLK
                     SEQNVTMRRYVKPTRTRQSSGRTSERADPTIGMGPRERARWERLRAWRTETAKSDGVP
                     AYVIFHDATLAEIARNGPDSIEDLRGIPGMGARKLDRFGDELLEVVAAD"
BASE COUNT          480 a          716 c          775 g          479 t
ORIGIN      
        1 ttccacaagg cacgcaagag caaggaactg tccgaccgcg agcgtttcga tcagatcgca
       61 gcggaagagt cgttcgaatt cggtacgccg gaaaccccgg ccgccgaaca gcagcactca
      121 ggcgagtaag tcctggcggc gtaagccgct tggggctcta gccagcgaag ccgctcagtt
      181 tcgactgagc ggtttttttt tgtcccacgc accgcgggcg cctcgctttt gtatgttttt
      241 gcggattcgt cggccgaacg gctaacgttg tcgcaaagcg cgagcgcagc acgaatgcgt
      301 tggtaaaatt cgtgtcaccg gctttacgct gcttctctca aatccatgtc ccgtccgctc
      361 gaaatcctca acgaagtctt tggctacccc gcgttcagag gacagcaggg cgaaattgtc
      421 gagcacgtcg ccagcggcgg cgactgcctc gtgctgatgc cgacgggcgg cggtaagtcg
      481 ttgtgctatc agattccgtc actggtgcgg cgcgaagctg ggtgcggagc gggcatcgtg
      541 gtgtccccgc tgatcgcatt gatgcaggac caggtcgccg cgctcacgga agtcggcgtg
      601 cgtgccgctt acctgaactc gacgctgtcg agcgcggagg cgatggccac tgagcgtgct
      661 ctacgtgaag gcgaaatcga tctgctgtat gtagcgccgg aacggttgat gacgcctcgt
      721 ttccaggagt tgctagaacg tacgcgtatt ggcctgttcg ccatcgacga ggcgcactgc
      781 gtgtcgcaat gggggcacga tttccgccct gaatatattc agctgtcggt gctgcacgag
      841 cgctttccga acgtgccgcg catcgcgctc accgcgaccg ccgacgcgat cacgcgcgac
      901 gaaatcattc accgtctcgc gctcgatgac gcgcgcatct ttgtctcgag cttcgatcgt
      961 ccaaacattc gctatcgcat cgtcgagaag gacaatgcgc gcacgcaatt gctcgacttc
     1021 attcgcgccg agcactccaa gccagacggc acgaccgacg ccggcgttgt ctactgcctg
     1081 tcgcgtcgta aagtcgaaga aacggcggag tggttgaaag agaaagggat gcgcgcgttg
     1141 ccttaccacg ccggcatgga gttcgaaatc cgccagaagc atcaggagat gtttcagcgt
     1201 gaggagggca ttgtgatgtg cgcgacgatc gcattcggca tgggaatcga caagccggat
     1261 gtgcgcttcg tcgctcacct tgatttgccg aagagtgtcg aaggttacta ccaggaaaca
     1321 gggcgcgcag gccgcgacgg catgccggcg aatgcctgga tggcgtacgg tctgggcgac
     1381 gtcgtgcagc agcgcaagat gatcgacgag tccgacgccg acgatgcgca taagcgcgtt
     1441 cagaccggca agctcgacgc gttactcggg ctgtgcgaag cggcgacctg ccgccgggtg
     1501 aggctgctcg cgtatttcgg cgaatcgagc aagccatgcg gcaactgcga caactgcatt
     1561 gagccgcccg acacatggga cgcgacgcgc gaagcgcaaa tggcgctgtc atgtgtgttc
     1621 cgcgcgcagc gggcgagtgg atttcacttt ggcgccggac acctgatcga tattctgcgt
     1681 ggcaaccgca gcgagaagat cctgcaacgc ggtcacgaga agctcaccac ctttgggatc
     1741 ggggcggcgc tcggcgagcc ggagtggcgc gccatctttc gccagctcgt cgcatttggc
     1801 tacctcaccg tcgaccacga aggcttcggc tcgctggtac tgactgaggc tgccaaagcg
     1861 gtgttgaaga gcgagcagaa cgtgacgatg cgccgctacg tgaagccgac gcgtacccgc
     1921 cagtcatccg gccgcaccag cgagcgtgcg gacccgacga ttggcatggg cccgcgcgag
     1981 cgcgcccgct gggaacggct gcgcgcctgg cgcacggaga cggcgaaaag tgacggcgtg
     2041 ccggcctacg ttatcttcca cgacgccacg ctggccgaaa tcgctcgcaa cggccccgat
     2101 tccatcgagg atttgcgtgg tataccgggt atgggtgccc gcaaactcga ccgtttcggg
     2161 gacgagttgc ttgaagtcgt agccgccgac tgagcgtcgg caagggcagt ttgaggtggc
     2221 caggcgtgcg aaacatcgca acctgttgac tcgtttagca ttttcggaat attatgctag
     2281 gttccggttc ttggaatgcc tgcgtgcgaa gtcaattcgt gcgctgacac ccggttccgg
     2341 aagttcgatg cgcaatcgtt tctgctttgc ccggaaacag atgcgtcgat tttgttcaat
     2401 ttcaggaata aacaatgcca accatcaatc aactggttcg caaaggccgc
//
LOCUS       DATASH010000873         8200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_345249,
            whole genome shotgun sequence.
ACCESSION   DATASH010000873 DATASH010000000
VERSION     DATASH010000873.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 8200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8200
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345249"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="VHV99_23325"
     CDS             complement(<1..854)
                     /locus_tag="VHV99_23325"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011544923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEX3638355.1"
                     /translation="MQKILVARPIFPDVIERLKQYFDVDWHQGDVLPVEELTRRLADK
                     DGALTAGDPIGAAVLAAAPRLRVVSNMAVGYNNFDMAAFNAANVLGTNTPDVLNESTA
                     DFGWALMMAAARRIAESEHWLREGKWQKWSYNSFLGSDIYGSTLGVIGMGRIGQALAR
                     RAKGFNMQVIYHNRSRVAPEIEAELNAQYASKQDLLQRADHVVLVLPYTKENHHTIGA
                     AELAQMKPTATLTNIARGGIVDDAALVEALRSKRIAAAGLDVYEGEPNLNPDLLSVPN
                     VVLTPHIA"
     gene            complement(922..2349)
                     /locus_tag="VHV99_23330"
     CDS             complement(922..2349)
                     /locus_tag="VHV99_23330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEX3638356.1"
                     /translation="MKRHAVWTSMVFVAALTLAGWPQLASAATLDGATLSALWGLPFA
                     GVLLSIAVFPLLAPAFWHHHFGKIAAAWALLFLVPFALTFGTALAFGTLVHAVLEEYI
                     PFIVLLAALYTVAGGICVRGNLHGTPRLNTAILALGTLLASIMGTTGAAMLLIRPLLR
                     ANDNRKHVVHVVVFFIFLVANAGGSLSPLGDPPLFLGFLQGVSFFWTTTHLAWPMLFV
                     CVVLLAAFYALDSYYFHRREEERSRFLDPTPDSPPLGIDGKINFVLLAAVIALVLMSG
                     LWKPDFAFDVFGTHVALQNAVRDVALIGVTLLSLALTPRAARAGNDFNWAPIEEVAKL
                     FAGIFVTIAPVITILRAGEAGAFAGIVHLVNPSGQPHDLMYFWATGLLSSFLDNAPTY
                     LVFFNLAGGDAQSLMTTGATTLAAISAGAVFMGANTYIGNAPNFMVKAIAESRGIRMP
                     SFFAYLGWSAAVLLPLFLVTGWLFF"
     gene            complement(2418..3080)
                     /locus_tag="VHV99_23335"
     CDS             complement(2418..3080)
                     /locus_tag="VHV99_23335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012363387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HEX3638357.1"
                     /translation="MDTSIARRNILARIRAAQGREPEPSASEREAAADYLARHPQGPR
                     PEMPADLITRFVEEAQKMATTVDTVEAPSEVPAAAYRYLTEHALPVQAIAWQTLQDLQ
                     WAEAGLNVEFRKPEDGDVVGLTGCFCATAETGTLALLSGPETYASAALLPETHIAIVP
                     ASRIVSGHEEAFGLIRSERGELPRAVNFVSGPSRTGDIEQTIVLGAHGPYRVHVIVVR
                     GA"
     gene            complement(3322..4518)
                     /gene="pncB"
                     /locus_tag="VHV99_23340"
     CDS             complement(3322..4518)
                     /gene="pncB"
                     /locus_tag="VHV99_23340"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0019358 - nicotinate nucleotide salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEX3638358.1"
                     /translation="MIITSLLDTDLYKFTMMQVVLHHFPAANVEYRFRCRTPNVDLVP
                     YIAEIRGEVRKLCELRFTDDELDYLRRMRFIKGDFIEFLALFHLNEKYISIEPSPKGN
                     GEIDIEIKGPWLHTILFEIPMLAIVNEVYFRNTQQKPDYSEGRDRLVEKIKLLGARPE
                     FADCKIADYGTRRRFSKQWHEEVILTLKAGLGEQFAGTSNVFYAMKHSLTPLGTMAHE
                     YLQACQALGPRLRDSQIFGFEMWAKEYRGDLGIALSDVYGMEAFLRDFDMYFCKLFDG
                     ARHDSGDPFDWGERLLKHYEANRCDPRTKILVFSDALDIPKVLQLYERFRGRCKLAFG
                     VGTNLTNDLGYNPLQIVIKMVRCNGQPVAKLSDSPGKNMCEDKAYLAYLRQVFGIAQP
                     EEDAAK"
     gene            4809..5132
                     /gene="fdxA"
                     /locus_tag="VHV99_23345"
     CDS             4809..5132
                     /gene="fdxA"
                     /locus_tag="VHV99_23345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HEX3638359.1"
                     /translation="MTHVVTESCIKCRYTDCVDVCPVDCFREGPNFLAIDPDECIDCA
                     VCVAECPVNAIYAEEDVPGDQQHFTELNADLAKNWPSITKTKASLPEADEFKDVKEKL
                     ALLAR"
     gene            5248..5323
                     /locus_tag="VHV99_23350"
     tRNA            5248..5323
                     /locus_tag="VHV99_23350"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5281..5283,aa:Asn,seq:gtt)
     gene            5386..5461
                     /locus_tag="VHV99_23355"
     tRNA            5386..5461
                     /locus_tag="VHV99_23355"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5419..5421,aa:Asn,seq:gtt)
     gene            5703..6179
                     /locus_tag="VHV99_23360"
     CDS             5703..6179
                     /locus_tag="VHV99_23360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HEX3638360.1"
                     /translation="MKPTLLRTALAVTTALLLTQAHSEEVGSVNTNFRVTGSDRVVVE
                     AYDDPLVQGVTCYVSRARTGGVKGTLGIAEDPTEASIACRQVGAINFTGPVKKQADVF
                     SVSMSLIFKSLHVVRIVDAKRSTLVYMTYSDRVVSGSAKNSVSAVPVPAGTTIPVK"
     gene            6404..7234
                     /locus_tag="VHV99_23365"
     CDS             6404..7234
                     /locus_tag="VHV99_23365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX3638361.1"
                     /translation="MMNAHRFQDSARYWRTPLLPGADLLTAEYHDHEFTPHWHDAYTI
                     PVIVAGAECYRYRGSDYVAEAGSVPIINPGELHTGSKAVEAGWRYRVMYAPVDFIHDL
                     ANEVAGKPQALPWFAPGVIRDHDLARRLARAHRLLEAGDDPLAAEAAMLDALSTLLAR
                     YSQTHPEPSRLAADDARVATMQERLTGDLAEALTLAEVARAAGLSPFHAARLFNQATG
                     LPPHAWRNQVRLQRSLAPLRAGVSVTEVAAAIGFTDQSHFTRHFRRMFGVPPGRWQGT
                     "
     gene            7573..>8200
                     /locus_tag="VHV99_23370"
     CDS             7573..>8200
                     /locus_tag="VHV99_23370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEX3638362.1"
                     /translation="MTHPNPGRPSSTGHLKEFTAGARDIIPMMVGAAPFGVIFGTLVA
                     SGPLHLWHGQLMSLVVFAGSAQFIALGLIAGHASFAVIWATTLVVNLRHVLYSATLAP
                     HVAHLSARWRWALGALLTDEVFAVAWEHYRHREPGTVGPYYFFGAGLAMYLNWQIWTV
                     AGLLFGAAFPGLQSLGLDFAMVATFIAIVVPQLVALGYIAAAATAGTLA"
BASE COUNT         1563 a         2625 c         2493 g         1519 t
ORIGIN      
        1 ctggcgatgt gcggcgtcag tacgacattc ggcacgctca gcaggtcggg attcagattc
       61 ggctcgccct catagacatc cagaccggct gcggcaatcc gcttcgaacg cagcgcctcg
      121 accagcgcgg catcgtccac aatgccgccg cgcgcgatgt tggtgagcgt tgcggtcggc
      181 ttcatctgcg cgagttcagc agcgccgatt gtgtgatggt tttccttcgt gtacggcaag
      241 accagtacca cgtgatccgc gcgttgcagc aggtcctgtt tggacgcgta ttgcgcgttc
      301 agctccgctt cgatctccgg cgcgacgcgc gaacggttgt gataaatcac ctgcatgttg
      361 aagcccttcg cacgacgcgc cagcgcttgc ccgatgcggc ccatgccgat cacaccgagg
      421 gtcgagccgt agatatcgct gccgagaaag ctgttatagc tccacttctg ccatttgccc
      481 tcgcgcaacc aatgctccga ttcggcgatg cggcgcgccg ccgccatcat caatgcccag
      541 ccgaaatccg cggtcgattc attcagcacg tccggcgtat tggtgccgag cacattcgcc
      601 gcattgaacg cggccatgtc gaaattgttg tagcccaccg ccatgttcga caccacgcgc
      661 agacgcggcg ccgccgcgag cactgccgcg ccgatcggat cgcccgcggt cagcgcgccg
      721 tctttgtccg cgagacggcg agtcagttct tcaaccggca acacgtcgcc ctggtgccag
      781 tcgacgtcga aatactgttt gagccgttcg atcacatcgg gaaagatcgg acgagcgacg
      841 aggatcttct gcattgcaat tctccgaata gcgcgtcgag ttaaatggtc tcggcgtcgc
      901 aaataagcat gaaacccggc gtcaaaaaaa cagccagccg gtgaccagaa acagtggcag
      961 caacaccgca gccgaccagc cgagatacgc gaaaaaactc ggcatgcgaa tgccccgcga
     1021 ttccgcgatc gctttcacca tgaagttcgg cgcgttgccg atatacgtat tggcgcccat
     1081 gaacaccgcg ccggccgaga tcgcggcgag cgtggtggcg ccggttgtca tcaggctctg
     1141 cgcgtcgccg ccggccaggt tgaagaacac caggtaggtc ggtgcattgt ccagaaacga
     1201 cgatagcagg ccggtcgccc agaagtacat caggtcgtgc ggctggccgg atgggttgac
     1261 cagatggacg atgccggcga acgcgcccgc ctcgccggcc cgcaggatcg tgatgaccgg
     1321 cgcgatcgtc acgaaaatgc cggcaaacag cttggcgacc tcttcgatcg gcgcccagtt
     1381 gaagtcgttg cccgcgcgtg ccgcgcgcgg cgtcaacgcg agcgagagca aggtgacgcc
     1441 gatcagcgcc acatcgcgca cggcattttg caacgcgacg tgcgtaccga agacgtcgaa
     1501 cgcgaagtcc ggcttccaga gcccgctcat caaaaccagc gcgatcacgg ccgcgagaag
     1561 cacgaagttg atcttgccat cgatgccgag cggcggtgaa tccggcgtcg gatcgagaaa
     1621 gcgcgagcgc tcttcttcac gccgatgaaa atagtacgag tccaatgcat agaacgcggc
     1681 cagcagcacg acgcagacga acaacatcgg ccacgccaga tgcgtggtgg tccagaagaa
     1741 actcacgccc tgcaggaagc cgagaaagag cggcgggtcg ccgagcggcg atagtgagcc
     1801 gccagcgttg gccaccagga agatgaagaa caccaccaca tgaacgacat gcttgcgatt
     1861 gtcattggcg cgcagcaatg ggcggatcag cagcatcgcc gcgccggtcg tacccatgat
     1921 gctggccaac aaggtgccga gagcgagaat ggcggtattc agccgcggcg tgccgtgcag
     1981 attgccgcgt acgcagatgc cgcccgccac cgtatagagc gccgccagca acacgatgaa
     2041 tggaatgtat tcttcgagca ccgcgtgcac tagcgtaccg aatgcgaggg ccgtgccgaa
     2101 agtaagcgca aacggcacca gaaacaggag cgcccacgct gccgcgatct tgccgaaatg
     2161 gtgatgccag aatgccggcg cgagcagcgg aaacaccgca atcgacaaca acacgcctgc
     2221 aaacggcaag ccccacagcg ccgataaagt ggcgccgtcg agcgtagccg ccgaagccag
     2281 ttggggccat cccgccagcg tcagtgccgc gacgaacacc atgctcgtcc atacggcatg
     2341 tcgtttcata tttttggaat ccttgttaca gcggacaact gagcgcgccg tcgcgcagat
     2401 accatctgtg gcccaactca ggcgccacgc acgacaatca catgcacccg ataaggccca
     2461 tgtgcgccaa gcacgatagt ctgttcgata tcgccggtgc gcgatggccc ggagacgaaa
     2521 ttgaccgcgc gcggaagttc accgcgttcg ctgcggatca ggccgaacgc ctcttcatgt
     2581 cctgagacga tgcgcgaagc cggcacgatc gcaatatgcg tttccggaag cagggccgcc
     2641 gatgcgtaag tctcgggccc ggacaacaga gccagcgtgc cggtctccgc agtcgcacag
     2701 aagcagccgg tgagtccgac cacgtcgccg tcttcaggct tgcggaattc gacgttgaga
     2761 ccggcttcgg cccactgtag atcctggagg gtttgccatg cgattgcctg caccggcaaa
     2821 gcatgttcag tgaggtagcg atacgcggcg gccggcacct cgctcggtgc ttcgaccgta
     2881 tcgaccgtgg tcgccatttt ctgcgcttct tcgacgaagc gcgtgatcag atcggccggc
     2941 atctccggac gcggaccttg gggatggcgc gccagataat ccgccgccgc ctcgcgctct
     3001 gaagcggacg gctcgggctc gcgcccctgc gccgcgcgga tgcgcgccag gatgttacgg
     3061 cgggcaatcg atgtatccat atgcgaaatc ctcgtgtcga cagccgtgcg atatggcgag
     3121 aattataccg gccaccccgc gcgcgaacac cctggccgaa tggcctatgg ccgcgtcctg
     3181 gcgacgcggc gagcgcaatt tccgtgaagc attggcacgt gagcggcaag cgaaccggcc
     3241 cctaccgggg gctcgccccc tcgacgtgcc tgcaacggtc actgtcttca cctgccgcac
     3301 aagagccgcg cgggcgcagc actacttcgc agcgtcttcc tcgggttgcg caatgccgaa
     3361 cacctgccgc agatacgcga gataggcctt gtcttcgcac atgttcttgc ccggcgaatc
     3421 ggacagcttc gccaccggct ggccattgca gcgaaccatc ttgatgacga tctgcaacgg
     3481 gttgtagccc agatcgtttg tcagattggt tcctacgccg aatgccagct tgcagcggcc
     3541 gcggaaacgc tcgtacagtt gcagcacctt cggaatgtcg agcgcgtcgg agaagacgag
     3601 gatcttggtg cgcggatcgc agcggttcgc ttcgtagtgc ttgagcaggc gctcgcccca
     3661 gtcgaacgga tcgccggaat cgtggcgcgc gccgtcgaac agcttgcaga agtacatgtc
     3721 gaagtcgcgc aggaacgcct ccatgccata cacgtccgac agtgcaatac ccaagtcgcc
     3781 gcgatattcc ttcgcccaca tttcgaagcc gaagatctgc gagtcacgca gccgcggacc
     3841 gagcgcctga cacgcctgca ggtactcgtg cgccatcgtg ccgagcggcg tgaggctgtg
     3901 cttcatcgcg tagaacacgt tgctggtacc ggcgaactgc tcaccgaggc cggctttcag
     3961 cgtgaggatc acttcttcgt gccattgctt cgagaagcgg cggcgcgtgc cgtaatcggc
     4021 gatcttgcag tcggcgaatt ccggacgcgc gcccagcagc ttgatcttct cgaccagacg
     4081 gtcgcgccct tcgctgtaat caggcttttg ctgggtgttg cggaagtaga cttcgttgac
     4141 gatcgcgagc atcgggatct cgaacaggat cgtgtgcagc cacggcccct tgatctcgat
     4201 gtcgatttcg ccgttgccct tcggcgacgg ttcgatcgat atgtatttct cgttcagatg
     4261 gaacagcgcg agaaactcga tgaagtcgcc cttgatgaag cgcatgcgcc gcaggtagtc
     4321 gagttcgtcg tcggtgaagc gcagctcgca cagcttgcgc acttcaccgc gaatctcggc
     4381 gatatacggc acgagatcga cattcggcgt gcggcagcga aaccggtact cgacattcgc
     4441 cgcgggaaag tgatgcaaca ccacttgcat catcgtgaac ttgtacagat cggtgtcgag
     4501 cagcgaagta ataatcatga tggtgcgaac tggactgcag gaaaaacgga aagcctggct
     4561 cgaccaaccg cgtcaaccca tgcgcgcgcg gcgcgccccg tgcgcgccga cttgacgcat
     4621 gttacccgaa tgcgtccgga ttcagcgcgc cctccgcctg ttgcgggact gttgcgccgg
     4681 acgccataac ctaatcacga aaacgtataa aaggcgttgc ccgctgccgt atgcggctct
     4741 tgacgttacg ttacaatagc gtttttggcg tttcgccttc cctgattccg catgcaggag
     4801 ctgcctgaat gactcacgtt gtgaccgaaa gctgcatcaa gtgccgctat accgactgcg
     4861 tcgatgtgtg cccggtggac tgctttcgcg aaggtcccaa cttcctcgcg atcgaccccg
     4921 atgaatgcat cgactgcgcc gtgtgcgtgg ccgagtgccc ggtgaatgcc atttatgccg
     4981 aagaagacgt gccgggcgac cagcagcact tcaccgagct gaatgccgat ctggcgaaga
     5041 actggccgag cattaccaag accaaggcgt cgctcccgga agccgacgaa ttcaaggatg
     5101 tgaaggaaaa gctggccctg ctcgcacgtt aagcagcatg gcccatgccc cgctgttgca
     5161 ttcgaatcaa atcgagatga acgcagggta ttgacaggtg cttaagctgc ttttacaatt
     5221 tcgcttctct gctgtttgtt gttctgttcc tcgatagctc agtcggtaga gcgccggact
     5281 gttaatccgt aggtccctgg ttcgagccca ggtcgaggag ccaagaattg caaaagcccg
     5341 ttaaccagta cgggcttttt tatgtagatc aagttgtact gctgttcctc gatagctcag
     5401 tcggtagagc gccggactgt taatccgtag gtccctggtt cgagcccagg tcgaggagcc
     5461 aaaaatttag aaggcccgca tccgtgcggg cctttttgtt ttggtcgttc tgttttggtc
     5521 tggcccattt cgatgcagga tcaagatgac tcgagtcggg gaacgaacgt ctatttcgac
     5581 cggcaggtag cgcatgacct cggtattcgc actgaccctc tggcggacac gccgttatac
     5641 gatggtgttt tgtcagcgca acctgctcgc gctcaaccca cgccctcctg cacggtcatc
     5701 ccatgaaacc caccttgttg cgcaccgcac ttgcagtcac taccgccctg ctgctgaccc
     5761 aagcacacag cgaagaagtc ggcagcgtca ataccaactt ccgcgtcacg ggctctgacc
     5821 gcgtggtcgt cgaagcctat gacgatccgc ttgtccaggg tgtgacctgc tacgtatcgc
     5881 gggcccggac cggcggcgtc aagggcacgc tcggcattgc cgaagatccg accgaagcgt
     5941 cgatcgcgtg ccgccaggtc ggcgcgatca acttcacggg ccccgtgaaa aagcaggccg
     6001 acgtgttttc cgtgagcatg tcgttgatct tcaaatcgct gcatgtggtg cggatagtcg
     6061 atgcaaagcg cagcacgctg gtgtacatga cctacagcga tcgcgtcgtc agcggcagcg
     6121 cgaaaaacag cgtgagcgcc gtgccggtgc cggccggtac gacgatcccg gttaagtgat
     6181 cgccgcccta tggcaaaaca cactgcacaa aggcatgaac ggcctgcgta acggcaccgg
     6241 cagcggccac ggcgcgctac actgagcagc catgccgggt ttgccgggtt tgccgggttt
     6301 gccggggttg ccggggttgc cggggttgcc ggggttgccg gggttgccgg gttgccgggt
     6361 tgccgggttg ccgggttgcc ggttgccgcc ccgccagagt tccatcatga atgcgcatcg
     6421 cttccaggat tccgcccgct attggcgtac gccgcttttg cccggcgcgg acctgctcac
     6481 ggccgaatat cacgaccacg agttcacgcc gcactggcac gatgcctaca cgattccggt
     6541 gatcgtcgcg ggcgcggagt gctatcggta tcgcggctcg gactatgtcg ccgaagcggg
     6601 cagcgtgccg atcatcaatc caggcgagtt gcacacgggt tcgaaggctg tcgaggcagg
     6661 ctggcggtat cgcgtgatgt atgcgccggt cgatttcatt cacgatctcg ccaatgaagt
     6721 cgccggcaag ccgcaagcct tgccatggtt cgcaccgggc gtgatccgcg atcacgatct
     6781 ggcgcggcga ctcgcgcgcg cgcaccggct gctggaggcc ggcgacgatc cgctcgccgc
     6841 cgaggccgcg atgctcgacg cgctgtccac cttgctggcc cgctattcgc agacgcaccc
     6901 ggaaccgtcg cggctcgccg ctgacgacgc ccgcgtcgcg accatgcagg agcgtctgac
     6961 tggcgacctg gccgaagctc tcacgctggc cgaagtcgcg cgagcagcgg gcctgtcgcc
     7021 gtttcacgcc gcacgccttt tcaaccaggc taccgggctg ccacctcacg cgtggcgcaa
     7081 ccaggtacgc ctgcaacgct cgctggcacc gttgcgcgcg ggggtctccg tgacggaggt
     7141 cgccgcagcg attggcttca cggatcagag tcatttcacg cgccacttcc ggcgcatgtt
     7201 cggcgtgccg ccgggacgct ggcaaggcac ctgaggcaac cctcacagga cggcaggata
     7261 ccgcccgcgg ggggtgtgtt tgcaacgcta tcgagccgcg tggccggtcg ttcagccatc
     7321 cccaacgcag caaagcaccc acactggtgg cccgcgcagg caacaccatg cagcgatgca
     7381 atcccagggc ctgcctctgc aggttccatt ccgattgggc cgcgctaggc ccgagccgcg
     7441 cgtcgcgcgt agcgcattgc atcagccccc ttaaccagac acggccctca gcagaatctg
     7501 cccgcccacc gcaagaacgt acaagccccc gcccccgccg ctcgctatgc ttccaaaatc
     7561 aaggaggcaa gattgaccca tcccaacccc ggccggccct cgagcacagg ccacttaaag
     7621 gaattcaccg ccggcgcgcg cgacatcatc ccgatgatgg tcggcgcggc gcccttcggc
     7681 gtgatcttcg gcaccctggt cgcgtccggt ccgctgcatc tatggcatgg ccagttgatg
     7741 tcgctcgtcg tattcgccgg ttccgcgcaa ttcatcgcgt tgggcctgat cgccggccac
     7801 gcgagttttg ccgtgatctg ggcgaccacg ctcgtcgtca acctgcgtca cgtgctgtat
     7861 agcgcgacgc tcgcgccgca cgtcgcgcat ctgtccgcgc gctggcgctg ggcgctcggc
     7921 gcgctgctca ccgacgaagt cttcgcggtc gcatgggagc actaccggca tcgggagccc
     7981 ggcacggtcg gcccgtacta cttcttcggc gcgggtctcg ccatgtatct gaactggcag
     8041 atctggaccg tcgccggcct gctgttcggc gccgcctttc ccggcctgca gtcgctcgga
     8101 ctcgatttcg cgatggtcgc caccttcatc gccatcgtcg tgccgcaact cgtcgcgttg
     8161 ggctacattg cggccgccgc gaccgccggg acgctcgcct
//
LOCUS       DATASH010000874         7431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_940838,
            whole genome shotgun sequence.
ACCESSION   DATASH010000874 DATASH010000000
VERSION     DATASH010000874.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7431
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940838"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VHV99_23375"
     CDS             complement(<1..250)
                     /locus_tag="VHV99_23375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX3638363.1"
                     /translation="MSAPIDSLQIGMHWFNERPGGLDRMFAALIDTLPAQGVNVRGMV
                     AGSPAVFDASGGSVLAFAGAQAPLGARLWGSRRQSRALR"
     gene            complement(247..1425)
                     /locus_tag="VHV99_23380"
     CDS             complement(247..1425)
                     /locus_tag="VHV99_23380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX3638364.1"
                     /translation="MRILIVTHVVRKNDGQGRVNYEIVLAALAAGHSVTLLAAEAAPD
                     LVQHPGVRFVRIAESRLPTRLVQYQLFAWRCGAWIRAHRQAFDVIHVNGFIAWARADV
                     NAAHFVHAGWYRCGFYPFRLLRGVYDAYQVLYTLLNIGCEKWAFRHAEVIVPVSHAVA
                     AEVRALGVGPARLQVIHNGVDIDEFAPGASERSRFDLPTAPFMLLFAGDLRMSRKNLD
                     TVLRALVYTSADVHLAVAGILHNSPYPALVASLGLTERVHFMDLVMDMPALMRSVDAF
                     VFPSRYEAMSLVLLEALAAGLPVITVRTAGGAEVITSRSGIVLDDPNDGAALAAAIER
                     LASDRAYARRLGQAAREVARSLSWQAMAERYLTVYEEVHARRTAQSLPAGCATASVEL
                     "
     gene            complement(1452..2996)
                     /locus_tag="VHV99_23385"
     CDS             complement(1452..2996)
                     /locus_tag="VHV99_23385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638365.1"
                     /translation="MARALVTPSTQVRLSTSTGKPRAFSGEAFASDMPSAPSRAARAQ
                     PKGLRHPLLLPLLLFAATLGLILLHQGRLVEYFFPLGAFGVASVLYWRSPAHYIGFVC
                     WLFFLTPEVRRLADFVNGAFNQQSPIMVATLLAVALTGLTLLKHVRLFGQRRAAPLVL
                     IVIALLYAFVVGMAQVGMAAATFTLINWLYPVMVAFYLTVTWRHYPAYHRVLLKTFIY
                     GGLLMSVYGLIEFISPMPWDAFWLISSKMLSEGQPVPFGMRVSSTMNSCGPFAVTLMT
                     ILLMSLAARGKARILLGCVGIPVLLLTSARSTWGGFAIALLYSFAMLDGKSRARLLAG
                     VLALAVVAAPLMMIDQVAEPVMLRLSTIQNLGQDDSYQARAEFYKSFMSSALTDIAGQ
                     GLGTTGLGSKLSDDKSAQAMLNFDSGLMEVPYVMGWPGTLLYTTGIVMLLWRAYRASR
                     RHPTDLLAISGVGVAVAIFSMMIFINTLTAVSGMFFFIGVTLPVISLRYARERHSPVA
                     ATRARP"
     gene            complement(2996..3529)
                     /locus_tag="VHV99_23390"
     CDS             complement(2996..3529)
                     /locus_tag="VHV99_23390"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3638366.1"
                     /translation="MNWKTLEFEQLSARELYLILRARSAVFVVEQSHVCLDADGRDES
                     ALHVFALEDVARPMPILAYARLQPGDAEDPEITIDKVLTSPLRRGDGTAEMLIERVLQ
                     AIAERWPGHAARVSAPLGLRDFYEQFGFRKTEGPYLEHGEPFIGLTRQVRGSPTLFGL
                     RRRERDGAAQVNTFELL"
     gene            3897..4148
                     /locus_tag="VHV99_23395"
     CDS             3897..4148
                     /locus_tag="VHV99_23395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX3638367.1"
                     /translation="MKTALRRILSESARLDVSPDTLADDADLYAAGLSSLATVHLMLA
                     IEDEFGIEIPDRMLTRRLFSSIDSMAAAVVELQQAKAAA"
     gene            4145..5443
                     /locus_tag="VHV99_23400"
     CDS             4145..5443
                     /locus_tag="VHV99_23400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX3638368.1"
                     /translation="MNAPLLDSAAMAAPLADAATEQAAVAGIVLSAVETEPGWRAAAQ
                     RCAAVATQFADAVDREARFPREAFDALKRERLLSAMVPAAFGGAGLSLADIGAICETL
                     AQGCASTAMVYAMHQIQVACIEAHGSESAWHRQLLPQLVEHQWLLASATSEETIGGNM
                     RTSACSVELDGEPGQQRFRIEKLAPTISYGAHADGILVTARRTAESAAADQVLIVALR
                     EATQLEKRGGWDSMGMRGTCSEGFRLVATGLAEQILPTPFAEIADQTMLPVSHTLWAS
                     VWTGVASDAVNRAKAFFRAQARSKPGAIPPAGLRLAEAVGLLQMMQARLSVALEAART
                     AHHAKHGGCQADAPLSAMLGFASDMNTLKTSISTTALQVVQEVLMICGMAGYKNGTEF
                     SVGRHLRDLYSAPLMINNDRIAQNTASLLLAQRPAAPGRA"
     gene            5445..6521
                     /locus_tag="VHV99_23405"
     CDS             5445..6521
                     /locus_tag="VHV99_23405"
                     /EC_number="6.2.1.n2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--[acyl-carrier-protein] ligase"
                     /protein_id="HEX3638369.1"
                     /translation="MNTMTDTAALAAATAAAAQETAAPSFRDELLAAGLLIDTGENGL
                     YGRSQIFEDVVERLNVAITHLGADQQPEVLRFPPAMRRTDFEDSEYLKSFPNLAGTIH
                     SFCGNDMGHQRLLRALDDAMTDSDDDRSDEWMAQQKPTRVVLTPAACYPIYPVVARRG
                     PLPADGRTIDVLSYCFRHEPSLDPGRMQMFRQREYVRLGSADQVMAFRQMWVERGSLL
                     ITLLQLPVEVDLANDPFFGRGGKIVADSQRAQALKFELLIPVADPRGKTACLSFNYHM
                     EHFGAIWKIACEDGAVAHTGCVGFGMERITLALFRHHGLDVNAWPADVRALLWGDTAG
                     RVAQGMQTMQAAATAAAESAGESA"
     gene            6518..>7431
                     /locus_tag="VHV99_23410"
     CDS             6518..>7431
                     /locus_tag="VHV99_23410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1839 family protein"
                     /protein_id="HEX3638370.1"
                     /translation="MNPSLIPAPKGGQSAFALPRRLDLPAQGGVTAPATALRQHAPHA
                     LHQGERVWQETNCYVDLWIELLHGFGLDPRAALGFTVTQDFEGDQFTFFKFPLEDLER
                     LYGTQVQELAIYDSLEARVLAQTSRGHTVLVEVDGYYLPNTRATSYRREHPKTTVGID
                     FIDPPARRLGYFHNTGYHLLDAEDYDGVFRKLPQFAQQPDLLFPYVEFAKQSRPALEG
                     TALAEASAELLCAHLGRRPLTNPISQWRAAFPAHLDMLLERGEAFFHPYSFNLMRQLG
                     ANFEFLSKYLLWLSAQGFEMPASIPAAA"
BASE COUNT         1404 a         2518 c         2337 g         1172 t
ORIGIN      
        1 cccgcagcgc ccgcgactgc ctgcgcgagc cccacagacg cgccccgagc ggcgcctgcg
       61 cgccggcgaa agccagcacg ctgccccccg atgcgtcgaa caccgcgggc gagcccgcca
      121 ccatgccacg gacattgacg ccctgcgccg gcagcgtatc gatcaacgcc gcgaacatgc
      181 ggtcgagccc gcctgggcgc tcgttgaacc agtgcatgcc gatttgcagc gaatcgatcg
      241 gcgcgctcat agctccaccg atgcggtcgc acagcccgcc ggcaacgatt gcgccgtgcg
      301 ccgcgcgtgc acctcttcat acaccgtcag atagcgctcc gccatggcct gccagctcaa
      361 gctccgggcg acttcgcgcg ccgcctgacc gaggcgccgt gcgtaggcgc gatcgctcgc
      421 gagacgttcg atcgccgcgg caagcgcggc cccgtcgttg ggatcgtcga gcacgatgcc
      481 gcttctggac gtaatcactt cagccccgcc cgcggtacgc acggtaatga ccggcaaccc
      541 cgcagccagc gcttcgagca gcaccaggct catagcttcg taacgcgagg gaaacacgaa
      601 cgcatcgact gaacgcatca gcgctggcat gtccatcacc aggtccatga agtggacccg
      661 ctccgtcaac ccgagcgacg ccaccagcgc cggataggga ctgttgtgca gaatccccgc
      721 gaccgccaga tgcacgtcgg ccgaggtgta cacgagcgcg cgcagcaccg tgtcgagatt
      781 cttgcgcgac atgcgcaagt cgccggcgaa cagcagcatg aagggcgccg tcggcaaatc
      841 gaagcggctc cgctcggacg cacccggcgc gaactcgtcg atatcgacgc cgttatgaat
      901 cacttgcagc cgcgccggcc cgacgcccag cgcgcgcact tcggcggcga ccgcatgcga
      961 taccggcacg atgacctccg catgccggaa ggcccatttc tcgcagccga tattgagcag
     1021 cgtgtagagg acctgatacg catcgtagac accgcgcaac aggcggaacg gatagaaacc
     1081 gcagcgatac cagccggcgt gaacgaaatg cgccgcattg acatcggcgc gcgcccacgc
     1141 gatgaagccg ttcacgtgaa tcacatcgaa cgcctgacga tgggcacgga tccacgcacc
     1201 gcaacgccat gcgaacagct gatactggac gagtctggtc ggcagccggc tttcagcgat
     1261 ccgcacgaag cgcacacccg ggtgctgcac caggtcgggg gccgcttcgg cggcgagcag
     1321 ggtcaccgaa tgaccggctg cgagcgctgc cagcacgatc tcgtagttga cacgtccttg
     1381 cccatcgttc ttgcgcacga catgggtgac gatcaaaatc cgcatcctgt tgtccttcct
     1441 ctggatattt ttcacggtcg tgcccgggtc gcggcgaccg gactgtggcg ttcacgcgcg
     1501 tagcgcaacg aaattaccgg cagcgtcacg ccgataaaga aaaacattcc cgacaccgcg
     1561 gtcaacgtgt tgatgaaaat catcatcgag aagatcgcca cggccacgcc gacgccggag
     1621 atcgccagca agtcagttgg gtggcgacgg ctcgcccgat aggcacgcca cagcagcatg
     1681 acgatgccgg tggtgtaaag cagcgtgccg ggccacccca tcacgtaggg cacctccatc
     1741 aggccgctgt cgaaattgag catggcctgc gcgctcttgt cgtcggacag cttgctgccg
     1801 agacccgtcg tgcccagccc ctggccggca atatcggtga gcgccgacga catgaacgat
     1861 ttgtagaact ccgctcgcgc ctggtagctg tcgtcctggc cgaggttctg aatcgtgctc
     1921 aagcgcagca tcaccggttc ggcgacctga tcgatcatca tcagcggcgc ggccaccacg
     1981 gccagcgcca gcacgccggc cagcagccgc gcccggcttt tgccgtcgag catcgcgaac
     2041 gagtacaaca gcgcgatcgc gaagccgccc cacgtgctgc gcgcgctggt gagcagcagc
     2101 acgggaatcc ccacgcagcc gagcaggatg cgggctttac cgcgcgcggc gagcgacatc
     2161 agcagaatcg tcatgagcgt gaccgcgaat ggcccgcatg aattcatcgt gctgctcacg
     2221 cgcatgccga acggcaccgg ctgcccctcg gagagcatct tcgaagagat cagccagaac
     2281 gcgtcccacg gcatcggcga gatgaactcg atcaggccgt acacactcat caggagtccg
     2341 ccataaatga aggtcttcag cagaacccgg tgataggcgg ggtaatggcg ccacgtgacg
     2401 gtgaggtaaa acgccaccat cacgggatac agccagttga tcagcgtaaa cgtcgccgcc
     2461 gccataccga cctgcgccat gccgaccaca aaggcataga gcaaggcgat cacgatcagt
     2521 accagcggcg ccgcacggcg ttgcccgaac aaccggacat gcttgagcaa ggtcaggccg
     2581 gtgagcgcga cggcgagcag cgtcgccacc atgatcgggc tttgctgatt gaacgcaccg
     2641 ttgacgaagt ccgcgaggcg ccgcacttcc ggcgtcagaa aaaacagcca gcagacgaat
     2701 ccgatgtagt gcgcgggcga gcgccaatac aacaccgacg ccacgccgaa cgcgccgagc
     2761 ggaaagaaat attcgaccag cctgccctga tgcagcagga tcaaacccag ggtcgcggca
     2821 aacaggagca acggcaacag gagcggatga cgcaagcctt ttggctgcgc tcgcgctgcc
     2881 cgggaaggcg cgctcggcat gtcagacgcg aacgcctcgc cggaaaacgc acgcggcttg
     2941 cccgtcgatg tcgagagtcg aacctgggtg gaaggcgtga ccagcgctcg cgccatcata
     3001 gaagctcgaa cgtgttgacc tgcgccgcgc catcgcgctc gcggcgccgc aggccgaaga
     3061 gcgtcgggct gccgcgcacc tggcgcgtca ggccgatgaa cggctcgcca tgttcgaggt
     3121 acggcccttc cgtcttgcga aagccgaatt gctcgtagaa gtcgcgcaag ccgagcggcg
     3181 cactcacccg cgcggcgtgt cccggccaac gttcggcgat cgcctgcagc acccgttcga
     3241 tcaacatctc agccgtgccg tcgccgcgcc gcagtgggct cgtcagcacc ttgtcgatgg
     3301 tgatttccgg atcttcggcg tcgccgggtt gcaggcgtgc gtaggccagg atgggcatgg
     3361 gccgcgccac gtcctcgagc gcgaagacgt gcagcgcact ctcgtcgcgg ccatcggcat
     3421 caagacagac atgcgattgc tcgaccacga agaccgcgct gcgcgcccgc aagatcaggt
     3481 acaactcacg cgccgaaagc tgttcgaatt ccagcgtttt ccagttcatc acgttcccca
     3541 gcggtaaata agttcggcga gcgcggcaac gctcaccgcc cttgatggcc tggctcgaca
     3601 cgccggcctg ttgtcatggc tacacggtac gtccgcgaaa cgcgtgtttc cgtgcgccat
     3661 cgcactgacg ctgggctttg acaattctta aatacgaggc gtcgggacat ggtgtagcga
     3721 ggagccgcca aaacagcttc agggcaccgt gaaagcgcgg caagcatgcg cgccgaaccg
     3781 ccaccagacg cgccgcgatg accccgcgcc gcgataaaca ccaaaaacga agacttctgt
     3841 tcgttttacg tcagcaacct tcaagagacc gttgaccacc aagggggcaa ctcaagatga
     3901 aaaccgcact gcgccgcatc ctttccgaat cagcccgtct cgacgtttcg ccggacaccc
     3961 tggccgacga cgccgatcta tatgccgcgg gcttgtcctc gctcgcgacc gtgcatctga
     4021 tgctcgccat cgaagatgaa ttcggcattg aaattccgga ccgcatgctc acgcgccggc
     4081 tgttctcgag catcgattca atggccgccg cggtcgtcga actgcaacag gcgaaggcgg
     4141 cagcatgaac gccccgctgc tggacagcgc cgcgatggcg gcaccgctcg ccgatgctgc
     4201 tacggagcag gccgccgtcg caggcatcgt gctgagcgcg gtggaaaccg aacccggctg
     4261 gcgcgccgcg gcgcaacgct gcgccgccgt ggccacgcag ttcgccgatg cggtggaccg
     4321 cgaagcgcgc tttccccgcg aagccttcga cgcgctcaag cgcgaacgtt tgttgtcggc
     4381 gatggtgccg gccgccttcg gcggtgcggg gctttcgctc gcggatatcg gtgcgatctg
     4441 cgaaacgctc gcgcagggct gcgcctccac cgcgatggtg tacgcgatgc atcagattca
     4501 ggtggcctgc atcgaggcac acggcagcga gtcggcgtgg caccggcagt tgctcccgca
     4561 actggttgag catcaatggc tgctggcttc ggcgacgtct gaagagacca tcggcggcaa
     4621 tatgcgcacc agcgcctgct cggtcgaact cgacggtgag cccggccagc agcgctttcg
     4681 catcgaaaag ctcgcgccga cgatttccta cggcgcgcac gccgacggca ttctcgtcac
     4741 cgcgcgccgc accgccgaat cggccgcggc cgaccaggtg ctgatcgtgg cgctacgtga
     4801 agcaacccag ctggaaaaac gcggcggctg ggattcgatg ggcatgcgcg gcacctgcag
     4861 cgagggcttc cggctcgtcg ctacgggtct cgcggagcag atcctgccga cccctttcgc
     4921 cgagatcgcc gatcagacca tgctgccggt ctcgcacacg ctgtgggcct cggtatggac
     4981 cggcgtggcc agcgacgcgg tgaaccgcgc gaaagccttc ttccgcgcac aggcccgctc
     5041 gaagcccggg gcgattccgc cggctggttt gcgcctcgcc gaggcggtcg gcctgctgca
     5101 gatgatgcag gcgcgcctct cggtcgcgct cgaagcggca cgcaccgcgc atcacgcgaa
     5161 acacggcggc tgccaggccg atgcgccgct ctcggcgatg ctcggcttcg cctccgacat
     5221 gaacacgctg aagaccagca tctcgaccac cgcactgcaa gtcgtgcagg aagtgctgat
     5281 gatctgcggc atggcgggtt acaagaacgg cacggaattc agcgtgggac ggcatctgcg
     5341 cgacctgtac tccgcgccgc tgatgatcaa caacgaccgt attgcgcaga acacggcgag
     5401 cctgttgctc gcacagcgtc ctgccgcccc ggggagagcc tgacatgaac acgatgaccg
     5461 ataccgccgc actggccgcg gcaactgccg cagccgctca ggaaaccgcg gcgccgagct
     5521 tccgcgacga actgctggca gcgggcctgc tgatcgatac cggcgaaaac ggcctgtatg
     5581 gccgcagcca gattttcgaa gacgtggtgg aacgtctgaa cgtagcgatc acgcatctgg
     5641 gtgcggacca gcagccggaa gtgctgcgct ttccgccggc gatgcgccgc accgatttcg
     5701 aagacagcga atatctgaag agctttccga atctcgccgg cacgatccac tcgttctgcg
     5761 gcaacgacat gggccaccag cgcctgttgc gcgcgctcga cgacgcgatg accgacagcg
     5821 acgacgatcg cagcgacgaa tggatggcgc agcagaagcc gacccgcgtg gtgctcacgc
     5881 cggccgcctg ctatccgatc tatccggtgg tggcgcggcg cggtccgctg cccgccgacg
     5941 gccgcaccat cgacgtgctg tcgtactgct tccgccacga accctcgctc gatccgggcc
     6001 gcatgcagat gttccggcag cgcgagtacg tgcgcctcgg cagcgccgac caggtgatgg
     6061 cgttccggca gatgtgggtc gaacgcggct cgctgctgat cacgctgctg caactgccgg
     6121 tggaagtgga tctcgccaac gatccgttct tcggccgcgg cggcaagatc gtcgccgata
     6181 gtcagcgtgc tcaggcgctc aagttcgaac tgctgattcc ggtggccgac ccgcgcggca
     6241 agacggcgtg cctgtcgttc aactatcaca tggagcactt cggcgcgatc tggaagatcg
     6301 cctgcgaaga cggcgcggtg gcgcatacgg gctgtgtcgg cttcggcatg gagcgcatca
     6361 cgctcgcgct gttccgccac catgggctcg atgtcaacgc gtggccggcc gatgtgcgcg
     6421 cgctcttgtg gggcgacacc gcggggcgcg tggcacaggg catgcagacc atgcaagcgg
     6481 cggccacggc ggctgcggag agcgccggag agagcgcatg aatccatcct tgatccccgc
     6541 gccgaagggc gggcaatccg ccttcgcgtt gccccggcgg ctcgacctgc cggctcaagg
     6601 cggtgtgacc gcgcccgcaa cggcgctgcg ccagcacgcg ccgcatgcgc tgcatcaggg
     6661 cgagcgcgtg tggcaggaga ccaactgcta cgtcgacctg tggatcgaac tgctgcacgg
     6721 cttcgggctc gatccacgcg cggcgctcgg ctttaccgtc acgcaggact tcgaaggcga
     6781 tcagttcacc ttcttcaaat tcccgctcga agacctcgaa cggctttacg gcacccaggt
     6841 gcaggaactg gcgatctacg actcgctcga agcccgcgtg ctcgcgcaaa cctcgcgcgg
     6901 ccacacggtg ctggtggaag tggacggcta ttacctgccg aatacgcgtg ccacgtcgta
     6961 tcggcgcgag catccgaaaa ccaccgtggg catcgacttc atcgatcctc ccgcgcgccg
     7021 gctcggctat ttccacaaca ccggctatca cctgctcgac gcagaagact acgacggcgt
     7081 gtttcgcaag ctgccgcaat tcgcgcaaca gcccgacctg ctgtttccgt atgtcgaatt
     7141 cgccaagcag tcgcggcccg cgctggaagg caccgcgctc gccgaagcat cggcggaatt
     7201 gttgtgcgcg catctgggcc ggcggccgct gaccaatccg atctcgcagt ggcgtgccgc
     7261 gtttcccgcg catctcgaca tgttgctcga gcggggtgag gcgttctttc acccgtactc
     7321 gttcaacctg atgcggcagc tcggcgcgaa cttcgagttt ctctcgaagt acctgctgtg
     7381 gctctccgcg caaggcttcg agatgccggc gtcgattccg gcggcggcgc a
//
LOCUS       DATASH010000875         1584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6083399,
            whole genome shotgun sequence.
ACCESSION   DATASH010000875 DATASH010000000
VERSION     DATASH010000875.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6083399"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            998..>1584
                     /locus_tag="VHV99_23415"
     CDS             998..>1584
                     /locus_tag="VHV99_23415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX3638371.1"
                     /translation="MTHTSQGISPLRQRMIDDMRMRQLSPKTQANYLRIVREFSRFLG
                     RSPDTATVEDLRGYQLHLVDHGTSPVSLNAAITGLKFFFEITLHEPDLMARMQPVRVP
                     STLPVVLSPDEVGRLIAATGNLKHQTALSVAYGAGLRASEVVALKVGDIDSQRMTLRI
                     EQGKGRKDRYAMLSPVLLERLRVWWRVAHAQGRMP"
BASE COUNT          290 a          477 c          524 g          293 t
ORIGIN      
        1 actggcgcgt gatattcccg cttaaatgcc gcgggcgtcg tacttcggcc attccgccag
       61 acgagcaccg tcggtgccgc tgatgggagt tcagggcact tgagtaatgc gttcgtcgga
      121 tggccgtcgc ggcctgccag atcgatttac gaaatcgaca tcatgtgagt tgaaagggta
      181 atggcggttt gatatcgagt tgaaaagaga tcttcgcgcg attcactgca atcgcaatat
      241 atcagcatat atagcgaggc gttgccttgc cgctcgtgcg ttccaggccg ttgcataggt
      301 gcttcgcgtg agttcgctac ggccgcgcaa tgttcgtgcg acgcctgaat ggacgcgcga
      361 cgaagcgcgg gtgttcaaca tggcgacgcc cgatgcgctc tttcagggaa ggtatccgga
      421 gtgccttcgc agcggctgac gctcgcgccg cagcgcggca agtggagcgt tggcattcgg
      481 cccgcagcac ggctgggcgc cggcggaagc gtcatggcag caacacgcat gtgcacgcgg
      541 caatttgtcg cgatcacgct tgctgcgact gattcttgat atcacgatcg agcaaagacc
      601 atcagataga tggcgcggga tcgaggtgtc gaggacgacg tggcgatatt tgccagcgcg
      661 gcgtgcggcc gatcaggctt gatgctgggc cagcggatcc tgctaacaga ccgtccgctc
      721 gtggtcgttg accattcgct ctcaacgccc gttgtctggc cggtaaggcc gaggacgcac
      781 gggtatgcgc gtcggggaga aggtccgtta ccaaggcgaa accgacgttc gaatgtccgg
      841 ctgagagcgc ggtcgagcgg ccgtagttgg ccggcgaacg aaaccgctcg cgcggtaagg
      901 gctccggcct gcgatagcgg taatggaggg gaaaccgact ctgttttcat gttgatgggc
      961 gaggcaatcc gcctcgtcag tcaacgggag cagagtcatg acccacacca gccagggcat
     1021 cagcccgctg cgccagcgca tgatcgacga catgcgcatg cgccagcttt cccccaagac
     1081 gcaggccaac tatctgcgca tcgtgcgcga gttttcccgg tttctcgggc gctcacccga
     1141 taccgctacc gtcgaggacc tgcgcggcta ccagttgcat ctggtggatc acggcacgtc
     1201 acccgtttcg ctgaacgccg cgatcaccgg cctgaagttc ttcttcgaga tcacgctcca
     1261 tgagcccgac ctgatggcgc ggatgcagcc ggtgcgtgtg cccagcacgc tgcccgtcgt
     1321 gctcagtccg gacgaagtgg gccgcctgat cgcggccacc ggcaacctga aacaccagac
     1381 ggcgctgtca gtcgcgtacg gcgcgggcct gcgcgccagt gaagtcgtcg cgctgaaggt
     1441 cggcgacatc gacagccagc gcatgacgct gcgcatcgag cagggcaagg gccgcaagga
     1501 ccgctacgcg atgctctcgc cggtgctgct cgagcgcctg cgcgtctggt ggcgcgtggc
     1561 gcacgcgcag ggcaggatgc ccga
//
LOCUS       DATASH010000876        21122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_345889,
            whole genome shotgun sequence.
ACCESSION   DATASH010000876 DATASH010000000
VERSION     DATASH010000876.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 21122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21122
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345889"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..1548)
                     /locus_tag="VHV99_23420"
     CDS             complement(34..1548)
                     /locus_tag="VHV99_23420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3638372.1"
                     /translation="MLATASATCALIVLDTNVVAVSLPSIARSFHASFADVEWVVSAY
                     MVAFASCLLPAGGLADRVGRKRMLLLGLAVFFLASLGCGAAPSAAMLNVARAVKGVGA
                     AMLLTAALAVIANTFHEGPARVRAWAVWGTCMGLATTVAPLVGGVITQWLGWRWIFLL
                     NLPVCAVLAWCASRSIRESRNPHPGPLDAAGSMLFGLALALGIWALIEVPADGLASWP
                     TGARLAACVLLLAAFVQVQRSRAHAMVDLALFRQPRFVAAVFAMFGYAACAQVMMTFL
                     PLYLQNAFGLSAVMAGVGMLPFALAMVLGPYIGAALARRMSGMALLSSGLLLIAAGNL
                     LTALVAGNARYALVALGMVVTGLGAGIMNGDTQKAIMACVPPDRTGMASGISTTTRFT
                     AIVTSVGVLGAVLAARTHAAFVAATQMTPDIGSALDAGFMSRVLAGDTAQAAAHLPPA
                     LRVILMSAAHASFASGFAAALYLAGAVAVSIAAGIWVLGRKGEAVRVSGVASNG"
     gene            1748..2623
                     /locus_tag="VHV99_23425"
     CDS             1748..2623
                     /locus_tag="VHV99_23425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3638373.1"
                     /translation="MRKLELRHIHAFVCVAKHLHFSRAADELDIAAPTLTKLIQEAER
                     LLGVRLFHRTKRSVALSAAGSAYLAEALSALGHLARGEERAILAERGELGRIEVGYVA
                     SAAYAGVLQAAVSGFRARYPGVDITIREVPMDSAADMLRDGLLDAAYVRPPLPLVDGI
                     QACTVHRDTFVLALPESSTLAGSPDIRPSQLRDQCFVLPEQEAGTFEVARRGRFSPRL
                     GPRPGTLAAVLACVSLGGNVAVVPHTLADCIALPGVVYRNIAGKPIASEIALAYRKFE
                     RAPAVRAFLEFARSR"
     gene            2823..3398
                     /locus_tag="VHV99_23430"
     CDS             2823..3398
                     /locus_tag="VHV99_23430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638374.1"
                     /translation="MSDPAGRLPNGLSSTIVAVKSASERGRAELIRDVVDAYRRLYGS
                     VQRFVLMLTDDQLNFESVGTLGSHSLNRLLSILAEQANAAAFVRLRDLKTSIEEARSA
                     EQLRDAIFSDASSNDLAALRKVVAELERLDAAFIGLCVGHVLEQHARAASDSQTAVVR
                     RAAGRTTAASAPAARRKSPSRAAPTLVRPND"
     gene            3539..4888
                     /locus_tag="VHV99_23435"
     CDS             3539..4888
                     /locus_tag="VHV99_23435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HEX3638375.1"
                     /translation="MHTPPFRLAQPSRLKLSGDELARTGLQATELCCTPAKVSFSNAK
                     RRARLAELDGQLHCSIIGTCLSTHELRKLVPKFTSLDRHDASDLEIHHSAVELAIDGG
                     AGAKALHKLLDEHYAAAIRRFDKAADEVELLKLWDEALKSGDVPPAYWALMTHPCATL
                     QVRQKAFGELHMLSHLVGAANRADIRRLVALEAENAELKEKIERQQNRLQEINTQRDA
                     SIAALNEQIAHLTALATRQTPTDPGDLEAEVARLRDKLADTDQRLALHTSRREAAEQR
                     VQQEQGAAFALRKSRDEALAQLLQVQSECDALERATVNAAAGYGVGVRQASLDGIRGK
                     RIVYVGGRPGSNAALRRLVEAAGGDFVVHDGGVEDRKGLLAAALPGADIVVFPVDCVD
                     HDSMSTLKRVCERHRIDYHPLRTASVASFVELVGRLRPEHLAQLGNPSPSAFCLRHG"
     gene            complement(4893..5291)
                     /locus_tag="VHV99_23440"
     CDS             complement(4893..5291)
                     /locus_tag="VHV99_23440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3638376.1"
                     /translation="MADTEKTKPRPATKSARPRPEVRFRMRIRTGDSVALGPGKVDLL
                     EAVREHGSISAAARSLDMSYRRAWLLIDELNRSLKAPATHSEQGGQSGGGCTLTPVGE
                     SIIRLYRDVEVEAQRCCAKQLAELTRLIRS"
     gene            complement(5341..5982)
                     /locus_tag="VHV99_23445"
     CDS             complement(5341..5982)
                     /locus_tag="VHV99_23445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006497847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifQ"
                     /protein_id="HEX3638377.1"
                     /translation="MSDEPLSPAADVAPPGADPLLTALDAQVAERTAEWLAAATNAAS
                     PDTQLFARLIAARDVRRELALLGLPPPAWHALLKRHFAHAPASVLAPRSALPPVDTRE
                     HAQFVATLHALLLTHSSTAVDANDAHCLASIIAHACLRPDHLWRDLGLAGREEVTWML
                     TRYFPALVVLNVGNLRWKKFLARQRALSVGLEPGPAPGCPRCEDYGHCFPKRH"
     gene            complement(6037..6525)
                     /locus_tag="VHV99_23450"
     CDS             complement(6037..6525)
                     /locus_tag="VHV99_23450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX3638378.1"
                     /translation="MDSVNVIAAPPLESALVLSGRFQRPLSFDLERLKAYESVEATPF
                     DLRCYTTNRFIRSVEPYRGVRLTTLILEAGLPNAEPVEFKRTVFVAVGHDGYAVTFSW
                     HELFNTPVGENVLIAYECGGRALDVEDGVPILFSGSDILPAPRHVKRLARIEAHVLKP
                     LA"
     gene            6621..7019
                     /locus_tag="VHV99_23455"
     CDS             6621..7019
                     /locus_tag="VHV99_23455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638379.1"
                     /translation="MSIRNISPGISATVGVAVSGDAVYVSDQANNDIVKSSLSALLGG
                     LQAVAPGAKFAQIDSPDLMAVDASGTLYTKCNTTGLCRIAPDGTICLLANDFQDARGM
                     AVDAPRRLLYAIDRAGSTGGTNYVRIFPLN"
     gene            7113..7988
                     /locus_tag="VHV99_23460"
     CDS             7113..7988
                     /locus_tag="VHV99_23460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011875863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX3638380.1"
                     /translation="MIRKLLASFVAVTGIEAAVPAFAQDNTVNVLYAGSLVNLMERSV
                     GPAFEKATGQQFRGYAAGSNKIANEIKGKLRRGDVFISASPTVNSSLMGEANGDHVTW
                     YVTFAESPLMIGYNPKSKFAADFKTKRWDQVLQEPGIRIGRTDPKLDPKGAFTVEMMT
                     KAAALYQQSDLVEKTLGDAENPAQVLPEETLVGRLQSGQLDAGFFYSTETSDLKIPAL
                     RPAPELQAKASYTLTILSDAPNRTGASSFVDFLLSAQGRALLKQHGVDVVKPTITGNA
                     QGVPPSVQAVIDGAQ"
     gene            8031..9869
                     /locus_tag="VHV99_23465"
     CDS             8031..9869
                     /locus_tag="VHV99_23465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013689494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3638381.1"
                     /translation="MKRSAARPLLWLAALLAVYLCAPFIASVPELGAADWPNVDWHAV
                     WSAVGISAASASVASLVILLGGVPLGYFLARSNSRKMALLGFVVQLPLALPPLTSGVL
                     LLFLLGPYSWVGRFTGGTLTDSFAGIVLAETFVAAPFLIIAAKSAFAAVDPVLDDVAA
                     TLGHHANSRFFRVILPIAWPAIRAGLALAWLRAFGEFGATVMVAYHPYSLPVYTYVVF
                     GGQGLPAMMPLLLPTLAIAIVCAALSIYSLRAKAAREQTENGDDTLEPGTDLTASRGE
                     TADLRLAFHLQRRLGAFELDIAWAPATRRLAIIGPSGSGKSLALRLIAGLESNASSDV
                     RLGTTDLSTLPPERRQIGYMPQDYGLFPHMTVAQQLAFPIDADAASARYWLDHLGLTQ
                     LVARLPHQLSFGQRQRVALARALTRHSELLLFDEPFAALDTPRRRRLQQSLRALQREI
                     TAVTIIVTHDPDEAALLADEVLVVEQGHVLQAGSIGAVFERPASMRVAELLGLHNVGV
                     GSMTAPRQIEIGNGLVIATGDSGSAMPAGQRVMWRISSHAVAISPDGAHAGVVDAVEL
                     RRGERYVRLNIAGVRFDIANEDARLREGSPCRIDVDASGVSVWNAS"
     gene            complement(9969..11411)
                     /locus_tag="VHV99_23470"
     CDS             complement(9969..11411)
                     /locus_tag="VHV99_23470"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEX3638382.1"
                     /translation="MQNDTSPLNAMASILSNPILNTDSYKASHYLQYPPEATAMFSYI
                     ESRGGRYDRTLFFGLQMLIKEYLCRPISAEMIGQAKAFFTGHGEPFNEAGWRYIVEHH
                     DGYLPVRIRAVPEGSVVPTHNVLVTVECDDPQVFWLASYLETMLLRVWYPITVATQSW
                     HLRKTIRAYLEKSSDDLTQLPFKLHDFGARGVSSAESAAIGGSAHLVSFMGSDTVLGV
                     VAANHYYNEPMAAFSVPAAEHSTITAWGRERETDAFRNMIAKFGKPGAIVSVVSDSYD
                     LFAALEVWGTDLKQAVLDSGGMLVIRPDSGDPLTIVLQTMRALEASFGSTVNGKGRRV
                     LNNVRVIQGDGVNPDSIEAILAAMDEAGFAADNIVFGMGGALLQQINRDTQRFAMKCS
                     AIRLGDEWHDVRKDPVTDAGKRSMKGRLTLLVNRHTGEYRTATLPLAWDDRTVEADWD
                     EALITVFDSGKLLVDVAFSEVRKRAHAGEG"
     gene            complement(11670..12872)
                     /locus_tag="VHV99_23475"
     CDS             complement(11670..12872)
                     /locus_tag="VHV99_23475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3638383.1"
                     /translation="MIDIFLISSSAERAPHIVARLDESGVVYRLRIAYCTLRQSRMHA
                     RAIRSADLLIVDDVDLSARDLDGVEETLACSPALHCLLVTPAPSTALLTAAMRVGVRE
                     VLSWPLDACEIAAALAHSAAKKNAGGHRAGRIVSLVSCKGGSGTTLIAVNLAYSLASL
                     RNSRVLLIDLSQQFGDASLLMADKPPPATLADLCSQIDCLDAALLDACVMRVHANLDV
                     LAGAGDPLKAAEVQPAQLEQVLMLVRERYDAVLIDIGQSLNPLTIHALDHSDAICMVV
                     RQNLLYLHGGRRMLDIFRELGYPSSKVRVVVNQYDKNAAINLPTLEQTLGAKVAHQLP
                     RDEKLVNEALNRGVPLVTSAHGSALAHGICLLADMLWPAIAEPRKGMPGRLFAARPDA
                     PPQLKPGH"
     gene            complement(12936..13223)
                     /locus_tag="VHV99_23480"
     CDS             complement(12936..13223)
                     /locus_tag="VHV99_23480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638384.1"
                     /translation="MKTNSKLAASRLDHHITAHRAALSYRRLSARQRALFAAPSADLL
                     TLVIAEAVKRKAQAEIQRDALRDAMFEMPVAPERGRDRGVDIYPADPDEYT"
     gene            14479..15915
                     /locus_tag="VHV99_23485"
     CDS             14479..15915
                     /locus_tag="VHV99_23485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX3638385.1"
                     /translation="MNTTCTPVEAERTVLYVSNSPDQDLASFLAASGWQAVHAKSTAV
                     AERMIERGNIKVGLVELPGDCTSQHLSALASCMRRVETNWVAQIAPGQAENELVSRFI
                     LDYCFDFVTRPWLNDRLVFALGHAHGLSSLRHAPVVPEPSLGRHGMVGQCEAMQQLYR
                     RIDKCGVTDAPVFVAGESGTGKELTARAIHERSPRAGRAFVAINCAAIPPSLLQAELF
                     GHERGAFTGALQRKIGRIESANEGTLFLDEIGDMPHECQAVLLRFLQEGTIERLGGNG
                     PINVDVRVISATHVDLDKAVEDGRFRSDLYHRLCVLRLVEPPLRERGGDIKLLANYAL
                     SMYRQDGARKLRGLSSDAIVAMSNYPWPGNVRELINCVRRAVVMSEGRFITASDLGLP
                     ETDHGPAVTLAEIRSKAEKDAIEHALQRHGYKLSEAAAELGISRATLYRLMHANRLHQ
                     EPAAGRASSANGGAESDDEAERHAPSLV"
     gene            16150..17973
                     /locus_tag="VHV99_23490"
     CDS             16150..17973
                     /locus_tag="VHV99_23490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3638386.1"
                     /translation="MADQHAPALVRRPGSVLPAQASPCDALAIRAQPLSFAHPCRRKK
                     LALAATILGSSMAFIDGSVVNVALPSIQTELGASVAAIQWVVNAYLLFLGALVLVGGS
                     LGDKLGRRTVFIAGIGLFTLASIGCGLAPGAGALIAARAVQGIGAALLVPSSLSIIGA
                     VFDDKERGQAIGTWAGVGAITSAVGPVAGGWLVDAFSWRAIFFLNVPVACATIALAIV
                     AVPDSRQEDDTLDLDPVLKASAQPNLDPALKASAQPKLDPALKASPQPKLDWLGAVTA
                     AAGLAALTYGLTIASARGFGDRLVLTSILGGLLVLSGFVALEAKTRNPMMPLDVFRSR
                     DFVGANLVTLLLYFGLGGALFFLPFTLIRAYGYSATEAGAALLPVPVTIGLLSRFTGG
                     LTSRYGARALLGTGPVIAALGFAMLALPWVRGEYWRGFFPALIVLGLGMTITVAPLTT
                     TVMSSVAAARTGVASGINNAVARVASLLAIAVLGIVFVRSHDAALDARLDALHVPQAA
                     RQAARLTPDSMDTSPAAAGASVASVASVASVASGASGASGASGAAAVAARAEVTHAQA
                     EALGAALQAVALASAGCALAGAALAALTIRAATGKTAVPQR"
     gene            18157..18495
                     /locus_tag="VHV99_23495"
     CDS             18157..18495
                     /locus_tag="VHV99_23495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HEX3638387.1"
                     /translation="MTITCFIRYQIDPFQRDAFDEYARNWGRIIPRCGGHLIGYFLPH
                     EGTDDIAWGLIAFNSLAAYEAYRATLRADPEARENFRFAQTKRFILREERTFTEVVDG
                     TLGVAAMGAM"
     gene            18737..>21122
                     /locus_tag="VHV99_23500"
     CDS             18737..>21122
                     /locus_tag="VHV99_23500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEX3638388.1"
                     /translation="MRKILHACFLVLLILGASPVFAAPAQPSAASAPSRAAVTLTPEQ
                     ARQALSVLNDPKRRAQVSDTLQAIAAAGALSAPPASAAAAASAPAAASSAATLVPAAF
                     QSNGLASQLARQGARWAAHLGVSLRGSVAALFDVASVRAWWNWQFTNPHARSVLAHVA
                     LSLLAALLPALVLEWLARRLLRRAYAVLAARRELRDEDSAAQADLPVDVLPQPANAPN
                     AAPPLPVSPGMAASADSPTSPASSTSSVSPASPASPAASASSVSSDATAQTAKPGASS
                     AAATSTTGKATEVKGKQSAVHHWTLLQRLPRALLTLVLRLLPLAVFVGATSALMSIFT
                     DDGTPQDRALDSLIDIYVLCRVIVIVSGFFLQPSAPRLRLLRMSDAWATFVQRWMVRI
                     VAVVGVGSAIAEIAQSLGLNDAAHLALMKVVALAGHIMISILILQCASPVGELIRRRF
                     AARESLQMLGNALADAWAWFAVFIVMALWFVWALDVQNGYHTLMHLGGITLAILVGAR
                     VASIVILGAMARLFHAQDDAEKSLAHRHAYRYYPLLRRMVGWIIGIVTALALLQVWGV
                     DVVDLFRSGSVAHRLASAIVTIGVAAVIALVVWESVNVSVERRLDRWTTSGDLVRAAR
                     LRTLLPMLRSALFCVIALVVVLTGLSELGVNVGPLLAGASIFGVALGFGSQKLVQDFI
                     TGIFLLMENAMQVGDWVTLAGVSGTVEYLSIRTVRLRGSDGSLYTVPFSSVSTVNNTN
                     RGLGNAAVKVNIVFGQDVDRAIDTLKEIGAALREDDKFKAGILSDFAFWGVDAVD"
BASE COUNT         3601 a         6923 c         6787 g         3811 t
ORIGIN      
        1 ccggattgcg cactgcatgg cgcgatcgcc tccctaaccg ttcgacgcaa ccccactcac
       61 ccggacagct tcgcccttgc gacccagcac ccagatgccc gctgcaatcg acaccgcaac
      121 cgcgccagcc agataaagcg ccgcggcaaa gccactggca aagctcgcgt gcgcggccga
      181 catcaatata acccgcagcg ccggcggcag atgcgcggcg gcttgcgccg tatcgccagc
      241 cagcacgcgc gacatgaaac ccgcgtcgag cgcgctgccg atatcaggcg tcatttgcgt
      301 ggcggcgacg aacgcggcgt gggtgcgggc cgcgagcacc gcgcccagca cacccactga
      361 ggtaacgatg gccgtgaagc gcgtcgtcgt gctgataccc gacgccatgc cggttcgatc
      421 aggcggcacg cacgccatga tggccttctg cgtatcgcca ttcatgatgc ccgcgcccag
      481 cccggtcacg accatgccga gcgccaccag cgcatagcgt gcgttgccgg cgaccagcgc
      541 cgtcagcaga ttgccggccg cgatcagcaa cagcccgctc gacagcaacg ccatgcctga
      601 catccgcctc gccagcgccg cgccgatgta cggcccgagc accatcgcca gcgcgaacgg
      661 cagcatgccg acgccagcca tcaccgctga caggccgaat gcgttctgca gatagagcgg
      721 caaaaacgtc atcatcacct gggcgcatgc ggcgtagccg aacatcgcga acacggcggc
      781 gacaaagcgc ggctggcgga acagcgcaag gtcgaccatc gcatgggctc gcgagcgttg
      841 cacctggacg aaggcggcaa gcagcagcac gcatgcggcg agtcttgcac cggtgggcca
      901 gctcgcaagc ccgtcggccg gcacctcgat caacgcccag atgccgagcg cgagtgcaag
      961 gccgaacagc atgctgccgg cggcatcgag cgggccgggg tgaggattcc tcgactcgcg
     1021 aatcgagcgt gacgcgcacc acgcgagcac ggcgcacacg ggcagattga gcaggaaaat
     1081 ccagcgccag ccgagccatt gagtaatcac accgccgacg agcggcgcca ccgtcgtcgc
     1141 aaggcccatg caggtgcccc acacggccca tgcgcgcacg cgtgcgggtc cttcgtgaaa
     1201 ggtgttggcg atcacggcga gcgcagcagt aagcagcatc gcggcgccga cgcccttgac
     1261 cgcgcgcgcc acgttcagca tggccgccga tggcgcggcc ccgcacccca gcgacgccag
     1321 gaagaacacc gcgagcccga gcagcagcat ccgcttgcgg ccgacgcggt cggcgagacc
     1381 gccggcgggc agcagacacg acgcgaacgc gaccatgtac gcgctgacca cccattcgac
     1441 atcggcgaaa ctcgcgtgaa acgagcgcgc aatcgacggc aaggagacgg ccacgacatt
     1501 ggtatcgaga acgatcagcg cacaggtcgc cgatgcggtg gccagcacga atgcggggtt
     1561 gacgggtttt gagccctttg agtcttcaga gccggagggc tgtgcgggcg gtgtcggacg
     1621 ggcggacggg gatggcatac gggcaggtgg aatcgaatcg aagtgattcg atggtagcga
     1681 agcgtctatt acctgtcatt ggcatagaat cacaaaataa ttagcgctga cggcaatact
     1741 gatgaccatg cgaaagctcg aactccgcca tatccacgcg ttcgtttgcg tcgcaaaaca
     1801 tctgcacttc tcccgcgccg cggatgaatt ggacatcgcc gccccaacgc taaccaagct
     1861 gattcaggaa gcggagcggc tgctcggcgt acggctcttc catcgcacca agcggtcggt
     1921 ggcattgagc gcggcaggca gcgcctacct cgccgaagcg ctcagcgcgc tcggccacct
     1981 cgcgcgcggc gaagagcgcg cgattctcgc cgagcgcggc gagttgggcc ggatcgaagt
     2041 cggttacgtc gcttcggccg cctacgcggg cgtcttgcaa gcggcggtca gcggttttcg
     2101 cgccaggtat ccgggcgtcg acattacgat tcgcgaggtg ccgatggaca gcgcagccga
     2161 catgctgcgt gacggcctgc tcgacgccgc ctacgtgcgc ccgccgttgc cgctcgtcga
     2221 cggtattcag gcatgcaccg tgcaccgcga tacgttcgtt ctcgcgctgc cggaaagctc
     2281 cacgctggcc ggctcgccgg acatcaggcc ctcccaactg cgcgatcaat gtttcgtgct
     2341 gccggagcag gaagccggca ccttcgaagt cgctcggcgc gggcgctttt cgccgcggct
     2401 cggacctcgt cccggcacgc tcgcggcggt gctggcctgc gtatcgctag gcggcaatgt
     2461 ggcggtcgta ccccatacgc tggccgattg catcgccttg ccgggcgtcg tctaccgaaa
     2521 catcgccggc aaacccattg cctcggaaat cgcccttgcc tatcgaaagt tcgaacgggc
     2581 gcccgccgtg cgggcgtttc tcgagttcgc gcgaagcagg taaaaccgcc cgcgctacac
     2641 gcgcggcgtc tttcgttata tcctgtcgaa tatcacggta tgcttcgtgg agcgcgctgg
     2701 cacgcccgtc aaatcgcgca cgacgaaaca aaacaagcaa gcaggagcca gcagcagtgc
     2761 ctagtcgatc cacctcccga tccacagcgc gagccacctc gcgatccaca ttgggatccc
     2821 cattgtccga cccggcggga cgcctcccga acgggctttc atcgaccatc gtcgcggtga
     2881 agtcggcatc tgaacgcggt cgcgccgaac tgatccgcga tgtggtcgac gcttaccggc
     2941 ggctttacgg cagcgtgcag cgcttcgtgc tgatgttgac ggacgaccag ctgaatttcg
     3001 aatcggtcgg caccttgggc tcgcactcgt tgaaccggtt gctgtcgatc ctcgccgaac
     3061 aggccaatgc ggcggctttt gtccgtttgc gcgatctgaa aacctcgatc gaagaagcgc
     3121 gttcagccga gcagcttcgc gacgcgattt tctcggacgc gtccagcaac gacctggccg
     3181 cgttacgcaa ggtggtggcg gaactcgaac ggctcgatgc ggcatttatc ggcctgtgcg
     3241 tcgggcatgt tctggagcag catgcgcggg cagcatccga ctcgcagacg gccgtcgtcc
     3301 ggcgcgccgc aggccgcact accgctgcct ctgctcctgc cgccaggcgc aaatcaccgt
     3361 cgcgcgctgc gcccacgctc gtccgcccca acgattaatg cgtcattgcc gcacatgaac
     3421 gttttctttc gcccgtcgtt atatcctttc gaatatatcg tcggcaatat gccgatggcg
     3481 agcgcattca gcccgctcgt tcagtcagcc tgacgccaga accggattcg aggtcctcat
     3541 gcacacgccg ccgtttcgtc tcgcccagcc ctcgcgcctc aaactgtccg gtgacgagct
     3601 tgcccgcacc ggcctgcagg cgaccgagct gtgctgcacc cccgccaagg tgtcgttttc
     3661 gaacgccaaa cggcgcgcgc gcctcgcgga actcgatggc caactgcatt gttcgatcat
     3721 cggcacctgt ctcagcacgc acgaactgcg caagctggtg cctaaattca ccagcctcga
     3781 tcgccacgac gcgagcgacc tggagattca ccactcggcc gtcgaactcg cgatcgatgg
     3841 cggcgcaggc gccaaagcgc tgcacaaatt gctcgacgaa cactatgccg cggcgatccg
     3901 ccgcttcgac aaagccgccg acgaagtcga gttgctcaag ctctgggacg aagcgctgaa
     3961 aagcggcgac gtcccgcctg cctactgggc tttgatgacg catccgtgcg cgaccttgca
     4021 ggtgcgccag aaagcgttcg gcgaattgca catgctgtcg cacctggtcg gcgcggcaaa
     4081 tcgcgcagac atccgcaggc tggtcgcact cgaagcggaa aacgccgaat tgaaagagaa
     4141 aatcgagcgg cagcaaaacc gcttgcagga gatcaacacg cagcgcgacg catcgatcgc
     4201 cgcgctcaac gagcagatcg cgcacctcac cgccttggcc acgcgccaga cgccgacaga
     4261 tcccggcgat ctcgaagccg aggtagcaag gctgcgcgac aagctggccg acaccgacca
     4321 gcggctggcg ctgcacacga gccgccgcga ggctgcggaa cagcgcgtgc agcaggaaca
     4381 aggcgcggcg ttcgcattac gcaagagccg ggatgaggca ttggcgcagc tcttgcaggt
     4441 gcaaagcgaa tgcgatgcgc tggaacgcgc aaccgtgaat gcagcggccg gctacggcgt
     4501 cggcgtacgg caagccagcc tcgacggcat acgcggcaaa cggatcgtgt atgtgggcgg
     4561 ccggcccggc tcgaacgccg cgctcaggcg gctggtggaa gcggccggtg gagatttcgt
     4621 ggtgcacgac ggcggcgtgg aagatcgcaa aggtctgctc gccgcggcgt tgccgggtgc
     4681 ggatatcgtc gtgtttccgg tcgactgcgt cgatcacgat tccatgagta cgctcaaacg
     4741 ggtgtgcgag cgccaccgga tcgactatca cccattgcgc acggcgagcg tggcgagctt
     4801 cgtggagttg gtgggccgcc tgcgccccga gcatcttgcc cagctgggca atccgtcgcc
     4861 gtcagcgttt tgcctgcgcc acggctgagc actcaggagc gaatcagcct ggtcagctcg
     4921 gcgagctgtt tcgcgcagca tctttgcgcc tcgacttcca cgtcacggta gagccggatg
     4981 atgctttcgc ccaccggcgt cagcgtacag ccaccgccgc tttgaccgcc ctgctccgag
     5041 tgcgtggcgg gcgccttgag cgaacggttc agctcgtcga tcagaagcca tgcgcgccgg
     5101 tacgacatgt cgaggctgcg cgccgcagcc gagatcgagc cgtgttcgcg cacggcttca
     5161 agcagatcga ctttgccggg tccgagcgcg accgaatcgc cggtgcggat gcgcattctg
     5221 aagcgcactt cagggcgcgg cctcgcggat ttcgttgccg gtctgggttt ggttttttcg
     5281 gtatcagcca tgcgcaaaag catacacgaa gcgcctcgcg ctgccatcct ggcatgggct
     5341 ctagtggcgc ttcgggaaac aatgcccata gtcttcgcag cgcgggcaac cgggcgcggg
     5401 gccgggctcg aggccgacgg aaagcgcgcg ttgccgggca aggaatttct tccagcgcag
     5461 attgccgacg ttcagcacga cgagcgccgg gaaataacgc gtcagcatcc atgtgacttc
     5521 ttcacggccc gcgaggccca ggtcgcgcca cagatggtcc ggccgcaggc acgcgtgagc
     5581 gatgatcgag gcgagacagt gggcgtcgtt cgcgtcgacg gcggtgctgg aatgcgtgag
     5641 cagcagcgcg tgcagcgttg cgacgaattg cgcgtgttcg cgggtgtcga cgggcggtaa
     5701 cgccgaccgg ggcgcaagca cggacgcggg cgcgtgtgcg aaatgccgct tcagtaatgc
     5761 gtgccaggcg ggtggcggca acccaagcaa ggcgagttcg cgacgcacgt cgcgcgcggc
     5821 aattagcctc gcgaacaatt gcgtatcggg cgacgccgcg ttcgtggccg ctgccagcca
     5881 ctccgccgtg cgctcggcaa cctgcgcatc gagcgccgta agcaacgggt cggcgcccgg
     5941 cggagcaaca tccgcagcag gcgaaagcgg ttcgtcagac atggcggcaa cgcgatgatg
     6001 aagagcggac catgttacgc ctgtcgctcg aatcccctag gccaaaggct tcaaaacatg
     6061 cgcctcgatg cgcgcgagac gtttgacgtg gcgcggcgcg ggcagaatgt ccgacccgga
     6121 aaacagaatc ggcacgccgt cttcaacatc gagcgcgcgg ccaccgcatt cgtacgcaat
     6181 caggacattc tcgccgaccg gcgtgttgaa cagttcatgc cacgagaacg tcacggcgta
     6241 gccgtcgtgc cccaccgcca caaacacggt gcgcttgaac tcaacgggct cggcgttcgg
     6301 caggcccgct tccagaatca gggtcgtcag acgcacgccg cgatacggct cgacgctgcg
     6361 gatgaaacga ttcgtcgtgt agcagcgcag atcgaacggc gtggcctcta cgctttcgta
     6421 agccttgagc cgctccaggt cgaaagataa ggggcgctga aaccgtccgc tcaggacgag
     6481 cgcgctttcg aggggaggcg cggcaatcac attcacacta tccataaacg gttatctcca
     6541 gcatcgatac ttgatgcagc ttgttcattc gttatgtccg tgtgtatatt acgctgcgat
     6601 gggcgcctcg gtgcaaaacc gtgtcgatac gcaatatatc gccaggcata tcggcgactg
     6661 taggcgttgc cgtatcgggc gacgccgtgt acgtctcgga tcaggcgaac aacgacatcg
     6721 tcaaatcgag tctgagcgcg ctgctcggcg gattgcaggc ggtggcgccg ggcgcgaagt
     6781 tcgcgcagat cgacagcccc gacctcatgg ccgtcgacgc aagcggcacc ctttacacca
     6841 agtgcaacac caccggcctg tgccggatcg cgccggacgg caccatctgc ctgcttgcaa
     6901 acgactttca ggacgcgcgc ggcatggccg tggacgcccc ccgccggctg ttgtacgcga
     6961 tcgaccgcgc cggcagcacg ggcggcacca actacgtgcg gattttcccg ctcaactgac
     7021 ctcactcgac ctcaccgaat gcgcagcaac ctgcaacatc acgcgatgac gtcgtaatat
     7081 agcggtctta ataacgcatc ggggttaatc gcatgattcg caagctgctc gcttctttcg
     7141 tggccgtcac tggcatcgag gcggccgtgc ccgccttcgc gcaggacaac acagtcaacg
     7201 tgctgtacgc cggttcgctc gtcaatctga tggagcgcag tgtcggcccc gccttcgaaa
     7261 aggccaccgg tcagcaattc cgcggctatg cggcgggttc gaacaagatc gccaacgaga
     7321 tcaaaggcaa gctgcgccgc ggcgacgtgt tcatcagcgc aagcccgacg gtaaacagca
     7381 gcctgatggg cgaggcgaac ggcgaccacg tcacgtggta cgtgaccttc gccgaatcgc
     7441 cgttaatgat cggctacaac ccgaaaagca agttcgcggc ggacttcaag accaagcgct
     7501 gggatcaggt gttgcaggag ccgggcatcc gtatcggccg taccgatccg aagctcgatc
     7561 cgaagggcgc ctttaccgtc gagatgatga cgaaggccgc ggcgctctac caacagtccg
     7621 acctggtcga gaagacactg ggcgacgcgg aaaatcccgc ccaggttttg cctgaagaaa
     7681 cgctggtggg ccgcctccaa tccggtcagc tcgatgccgg tttcttctac tcgaccgaaa
     7741 cctccgacct gaaaatcccg gcgctccgcc cggcgcccga attgcaggcc aaggccagct
     7801 acacgctgac gattctcagc gatgcaccga accgtacggg cgcgagcagc ttcgtcgact
     7861 tcctgctgag cgcgcagggc cgcgcgctgc tcaagcaaca cggcgtggac gtcgtcaaac
     7921 cgaccattac cggcaacgcg caaggcgtgc cgccttcggt gcaagccgtg atcgacggcg
     7981 cgcagtaagg cgtggtgaaa cagatggtga aacagatggt gaaacaggcg gtgaaacggt
     8041 ccgccgcgcg accgctattg tggctggcgg cgctgctggc cgtctattta tgcgcgccgt
     8101 ttatcgcgag cgtgccggag ctcggcgcgg ccgattggcc gaatgtcgac tggcacgcgg
     8161 tgtggtcggc ggtcggcata tcggcggcga gcgccagtgt ggcctcgctc gtgattctgc
     8221 tgggcggcgt gccgctcggc tattttctgg cgcgctcgaa ctcacgcaag atggcgctgc
     8281 tcggcttcgt cgtgcagttg ccgcttgcgt tgccaccgct cacgagcggc gtgctgctgc
     8341 tgtttctgct gggcccgtat agctgggtcg gccggttcac gggcgggacg ctgacggatt
     8401 cgttcgccgg tatcgtgctg gcggaaacct tcgtggccgc gcccttcctg atcatcgccg
     8461 cgaaatcggc gttcgccgcc gtcgatcccg tactcgacga cgtcgccgcg acgctcggcc
     8521 atcatgcgaa cagccgcttt ttccgtgtga tcctgccgat tgcgtggccg gcgattcgcg
     8581 cgggacttgc gcttgcctgg ttgcgtgcgt tcggcgaatt cggcgcgacg gtgatggtgg
     8641 cctatcaccc ctattcgctg ccggtgtaca cgtatgtcgt gttcggcggc cagggcttgc
     8701 cggcgatgat gcctctgctg ttgccgacgc tcgcgattgc gattgtctgc gcggcgctgt
     8761 cgatttatag cctgcgtgca aaagcggcgc gcgaacaaac cgaaaacggc gacgacacgc
     8821 tggaacccgg cacggatcta acggcgagcc gcggcgagac cgccgatctg cgcctcgcat
     8881 ttcatttgca gcgccgcctg ggcgcgttcg aactcgacat agcgtgggcg cctgcaacgc
     8941 ggcggctggc gatcatcggg ccgtcgggct cgggcaaatc gttggcgttg cgtctgattg
     9001 cggggctcga atccaacgcg tcgagcgacg tgcggctcgg cacaaccgac ttgagcacac
     9061 tgccgcccga gcgccgccag atcggctaca tgccgcaaga ctacggtttg ttcccgcaca
     9121 tgacggtcgc gcagcaactc gcgtttccta tcgatgccga cgccgccagc gcgcgttact
     9181 ggctcgatca tctcggcctc acacaactgg ttgcgcgctt gccgcatcaa ttgtcgttcg
     9241 gccaacggca acgcgtggcg ctggcgcggg ccctcacgcg tcatagcgaa ttgttgctgt
     9301 tcgacgagcc gttcgcggcc ctcgatacgc cgcggcggcg gcgtttgcag cagtcgctgc
     9361 gggccttgca gcgcgagatt acggcggtga cgatcatcgt cacgcacgat cccgacgaag
     9421 cggcgctgct tgctgacgag gtgctggtcg tcgaacaggg gcacgtgctg caggcgggtt
     9481 ccatcggcgc ggtgttcgaa cggccggctt cgatgcgcgt tgcggaactg ctcggcttgc
     9541 acaacgtggg cgtggggtcg atgactgcgc cccggcagat cgagatcggc aacgggctgg
     9601 tgatcgcgac aggcgatagc ggttccgcca tgcctgccgg gcaacgggtg atgtggcgca
     9661 tctcttcgca cgccgtggct atttcacccg acggcgcgca tgccggcgtg gtcgacgctg
     9721 tcgagttacg gcgcggcgag cgatatgtcc ggttgaacat cgcgggggtg cgtttcgata
     9781 tcgcgaacga agatgcccgg ctgcgggaag gaagcccttg ccggattgac gtcgacgcct
     9841 ccggtgtttc ggtctggaat gcgagctgac cggttggttc cggcgctggc agcggcatgg
     9901 cgattgggcc gcctcgccct cgccgctgcc ctgctgctgc cccgccgctg cgagcgccaa
     9961 acgcccgatt aaccctcgcc cgcgtgtgcc cgtttcctga cttcggaaaa cgcaacgtcg
    10021 acgagcaatt tcccggaatc gaaaacggtg atgagcgcct cgtcccagtc cgcttcaacg
    10081 gtacggtcgt cccacgccaa aggcagcgtc gccgtccgat actcgcctgt gtggcgattc
    10141 accagcaacg taagccggcc cttcatcgac cgcttaccgg catccgtcac ggggtccttg
    10201 cggacgtcgt gccattcatc cccgagccgg atggccgagc acttcatggc gaagcgttgc
    10261 gtatcgcgat tgatctgctg cagcaacgcg ccacccatgc cgaacacgat gttgtccgct
    10321 gcaaaaccgg cctcatccat ggccgcgagg atcgcctcga tcgaatccgg attcacgccg
    10381 tcgccctgaa tcacgcggac gttattgagc acgcgccgcc ccttgccgtt caccgtcgaa
    10441 ccaaacgatg cctcgagcgc ccgcatggtc tgcagcacga tcgttagcgg atcgccggaa
    10501 tcaggccgga tcaccagcat gccgccggaa tcgagcaccg cctgcttgag gtccgtgccc
    10561 catacctcga gtgcggcgaa caggtcgtag gaatcggaca ccaccgagac gatcgccccc
    10621 ggcttgccga acttcgcgat catgttgcgg aacgcgtccg tttcgcgctc gcgcccccat
    10681 gcggtgatgg tgctgtgctc cgcggccggc accgaaaacg ccgccatcgg ttcgttgtaa
    10741 tagtggttcg cggcgaccac accgagcacc gtgtccgagc ccatgaaact gacgagatgc
    10801 gccgagccgc cgattgccgc cgactccgcg ctcgacacgc cgcgcgcgcc gaaatcgtgc
    10861 aacttgaacg gcagttgcgt cagatcgtcg ctactcttct ccagataggc gcggatcgtc
    10921 ttgcgcagat gccagctttg cgtcgccact gtaatcggat accacacgcg caacagcatc
    10981 gtttccagat acgaggcgag ccagaacacc tggggatcgt cgcattcgac cgtgaccagc
    11041 acattgtgcg tgggcaccac cgagccttcc ggcaccgcgc ggatccgcac gggcagatag
    11101 ccgtcatggt gctcgacgat gtagcgccag ccggcttcgt tgaacggctc gccgtgcccc
    11161 gtgaaaaacg ccttcgcctg gccgatcatc tccgcgctaa tggggcggca cagatactcc
    11221 ttgatcagca tctgcagacc gaagaacagc gtgcggtcat agcgcccgcc gcgcgactcg
    11281 atgtacgaaa acatcgccgt cgcttcaggc ggatattgca ggtaatgcga cgccttgtac
    11341 gaatccgtgt tgaggatcgg attggacagg atcgacgcca tggcgttgag tgggcttgta
    11401 tcgttttgca tgggtgatgc agcgcgcgtg cggttctctc aagagtaatc aggtgggctc
    11461 ggggacgtgg cgcttacgtc gtcggtgaaa ctacaccata caccgccaga acacgcccgg
    11521 caccgcacct tacctcgttg cgcccgatgc cgcttgccgt cggacagagg gtgttcttcc
    11581 cgcacaatgc gctcgacctg cagccggctg tccatgtcat gccacgcgcg ccgcatgcca
    11641 ttccggcgag acgcggcaag cggccgggtt cagtgcccgg gcttcagttg cggcggcgca
    11701 tccggccgtg ccgcaaacag gcgtccgggc atgcctttgc ggggctcggc gatagccggc
    11761 cacagcatgt cggcgagcag acagatgccg tgcgccaatg cgctgccgtg ggcggaggtg
    11821 acgagcggca cgccgcggtt gagtgcctcg ttgaccagct tttcgtcacg cggaagctgg
    11881 tgcgcgactt tcgcgccgag cgtctgttcg agcgtgggca ggttgatggc tgcgttcttg
    11941 tcgtattgat tcaccaccac ccgcaccttg ctggacggat accccagttc cctgaaaata
    12001 tcgagcatgc ggcgcccgcc gtgcaggtaa agcaggttct gccgcaccac catgcagatg
    12061 gcatcgctat gatcgagtgc atggatcgtc agcggattga ggctttgacc gatgtcgatc
    12121 agtactgcgt cataccgttc gcgtaccagc atcagaactt gctccagttg cgcgggctgc
    12181 acttcggctg ccttgagcgg gtcgccggcg cccgccagca cgtccagatt cgcgtgtaca
    12241 cgcatgacac atgcgtccag caaggcggcg tcgaggcagt cgatctgcga gcacagatcg
    12301 gcaagcgtcg cgggcggcgg tttgtcggcc atcagcaggc tggcatcgcc gaattgctgg
    12361 ctgagatcga tcagcagcac gcggctgttg cgcagcgagg ccagggaata ggcgagattc
    12421 acggcgatca gcgtcgtacc gctaccgccc ttgcacgaga caagcgagac gatccgcccg
    12481 gcgcggtgcc cgcctgcgtt ctttttcgcc gcgctatgcg cgagcgccgc ggcgatctcg
    12541 caggcatcca gcggccatga cagcacctcc ctcacgccga cgcgcatcgc ggcagtcagc
    12601 aaggccgtgg atggcgccgg cgtgacgagc aggcaatgca aggcaggcga gcaggccagc
    12661 gtctcctcga cgccgtccag atcgcgcgcg ctcaaatcga catcgtcgac gatcagcaaa
    12721 tcggcgctcc tgattgcgcg ggcgtgcatg cgcgactgcc gcagcgtgca gtacgcgatg
    12781 cgcaagcgat acaccacgcc gctttcgtcg agacgcgcga cgatatgcgg cgcacgctcc
    12841 gcgctggacg aaatcaggaa gatgtcgatc atgccagggt ctccgccggc ctgaggcctg
    12901 tcagttggca aggccgcgtc aatcacagtc gacgctcagg tgtactcgtc gggatcggct
    12961 gggtagatgt ccacgccgcg atcccggcct cgttcgggcg caaccggcat ctcgaacatc
    13021 gcgtcgcgca gcgcatcgcg ttgaatctcg gcctgagcct tgcgtttcac cgcctcggcg
    13081 atgaccagtg tgagcagatc ggccgacggc gcggcgaaca aggcgcgctg ccgcgccgaa
    13141 agacgccgat aggacagagc ggcgcggtgc gccgtgatgt ggtggtccag ccgcgatgcg
    13201 gccagcttgc tgttcgtttt catgacaccc ccgaatgatg gaccctttgc ggaccgcatc
    13261 caggtaagcc gtgcctgcgc taatgcgcgg gcacggcgcc cggttcacct cgtaacatgc
    13321 gcccctctat ggagactcag gccgttcctc cccgcttcgt cgcgccggcc ggtcctgacc
    13381 gcgctgcccc ttccctgttc tgtgcgtcgc gtgcctgtaa attgcattga cacggtacct
    13441 gttgcttttt ccacttattc atttcggcta gctacattgg ccagctactt cggtcgtcca
    13501 cttcgagtcc gtccagcttg acggaaaaag tggcgaggtc agcgcttcac gatgtcgggt
    13561 gacgaatgac cgaaactccg ttccgatcat tcaccccgtt tttttgacat ccgcttttca
    13621 gtggccccgc cgcaccctcg caagacacat tggcatcccc ggccgcgctt acgaagacga
    13681 tgtttgccgc cgggctccag ctgctgtttc cccgtgcttc cggaccccgg cggcaatgca
    13741 ttggcttgcg aagctcttat tgcaatggct gtgccaagta ccttcaatcc cttgtcgcac
    13801 ggcgatttct gctgcaacgc ggtctgtccg gggcggcttg ccgtcacgcg ttgattcgga
    13861 ttcgacacgc aattgattca ccgcaagcgc agggcgggac gatctacctg tccgcgtgat
    13921 tgggcacggc cctcatccca ccctgtcagg ccgcgctttg cggcgcagcc cggtcaaatg
    13981 tgccgatact tcgggacacg aagcagcact tggagccgct gcccgacatg tcgcaggctt
    14041 gagacgcggc ggctcgatga tccggcatcc gtttgcacga tgattgccat gcgttttgcc
    14101 gcgctctgtg gtacacctcg catagccggg gttgacacta tttttgaacg gccagcaaac
    14161 acctcgcagc aggaaaaacg tttctgaatc ccgatttgtt tgtatggcgt tcgaaagcac
    14221 tttcgcttat ggttagtgca tgcgccgcat agagcgcctg tgtcagtcca ccgaggtaaa
    14281 aaacggctgt tgtgattcgg ggatgaaata gtctgacggg ttcagcgccg atcgctgttc
    14341 ccgttatctg cccgctgcgc gtcgcggccc gtgccgccgc tccatgcccc cgccattgcc
    14401 gctaccgtga tttgtcattg ccggtccgac cagtacgtcc aagcgcagtt ctgagagcag
    14461 caacgatctg ggggttacat gaatacgact tgcacgcctg tcgaagccga acgcaccgtt
    14521 ctgtatgtgt cgaactcgcc cgatcaggac ctcgccagct tcctcgcggc atcgggctgg
    14581 caagcggtgc acgccaaaag cacggcggtc gccgaacgca tgatcgaacg cggcaacatc
    14641 aaggtcggcc tggtcgaatt accgggtgac tgcacgtcgc agcatctgtc cgcgctggcc
    14701 tcctgcatgc ggcgcgtcga aaccaattgg gtggcgcaga tcgcgccggg ccaggcggaa
    14761 aacgaactcg tcagccgctt cattctcgac tactgctttg atttcgttac ccggccgtgg
    14821 ctgaacgatc ggctcgtgtt cgcgctcggc cacgcgcacg ggctatcgag cctgcggcat
    14881 gcgccggtcg tgccggagcc gtcgcttggc cggcacggca tggtgggaca atgcgaggcg
    14941 atgcagcagt tgtaccggcg tatcgacaaa tgcggcgtga ccgatgcgcc ggtgttcgtc
    15001 gccggcgaat cgggcaccgg caaggagctg accgcccgtg cgattcatga gcgctcgccg
    15061 cgcgcgggcc gggcgttcgt cgcgatcaat tgcgcggcca ttccgccgag cctgctgcaa
    15121 gccgaattgt tcggccacga acgcggcgcc tttaccggcg cgttgcaacg caagatcggc
    15181 cggatcgaaa gcgctaacga gggcacgctg tttctggatg aaatcggcga tatgccgcat
    15241 gaatgccagg cggtgctgct gcgcttcctg caggaaggca ccattgaacg gctgggcggc
    15301 aatggaccga tcaacgtcga cgtgcgggta atttcggcga ctcacgtcga tctggacaaa
    15361 gccgtcgaag atggccgctt ccgctccgac ctgtatcacc gcctgtgcgt gctgcggctc
    15421 gtcgagccgc cgttgcgcga gcgcggtggc gacatcaagc tgctcgccaa ctatgcgctc
    15481 agcatgtacc ggcaggacgg cgcgcgcaaa ctgcgcggac tttcgagcga cgcgatcgtc
    15541 gccatgtcga actatccgtg gccgggcaac gtgcgcgagc tgatcaactg cgtgcgccgt
    15601 gccgtggtca tgagcgaggg gcgctttatc actgcaagcg atctcggctt gccggaaacc
    15661 gaccacggcc cggctgtgac gctggcggaa atccgcagca aagccgaaaa ggacgccatc
    15721 gagcatgcat tgcagcggca tggctataaa ttgtccgagg ccgccgctga gctcggcatt
    15781 tcgcgggcaa ccctgtatcg cctgatgcat gcgaaccggc tgcatcagga accggcggcc
    15841 gggcgcgcgt cgagcgccaa cggcggcgcg gagtccgacg acgaggcgga gcggcacgcg
    15901 ccatcgctgg tctgacttcg taggccgcct aagcggggca agcctttttg ctcatcgaac
    15961 cgtagcgctt ttatcccgcg catccggtgc cgatgcgatg ggtctcgcat ggccgcgttc
    16021 ccggacaacg ctcaaatccc atggttccgt gccggttgcc gcggcccggc gcagcggacg
    16081 tcatgagcgc ctatcggatc gccccaggcg ttgctatgct ggaggccgtt catccatctg
    16141 ggagtggact tggccgatca gcacgctccg gcgcttgtac gccgccccgg ctccgttctc
    16201 cccgcccagg cgagcccgtg cgacgcgctc gccattcgcg ctcagcccct tagcttcgct
    16261 cacccgtgcc ggcgcaaaaa actcgcgctc gccgcgacca ttctcggctc gagcatggcg
    16321 ttcatcgacg gctccgtcgt caacgtcgcg ctgccgtcga tccagaccga gctgggcgcg
    16381 agcgtcgcgg cgatccagtg ggtggtcaac gcgtatctgc tgtttctcgg cgcgctggtg
    16441 ctggtgggcg gctcgctcgg cgacaagctc ggccggcgca ccgtgtttat cgcgggaatc
    16501 ggcctgttca cgctggcctc gatcggctgc ggcctcgcgc ccggcgccgg cgccctgatt
    16561 gcggcgcgcg ccgtgcaagg catcggcgcg gcgttgctgg tgccgagcag tctcagcatt
    16621 atcggcgcgg tgttcgacga caaggagcgt ggccaggcga tcggcacctg ggccggcgtc
    16681 ggcgcaatca cgtcggcggt cgggccggtg gcgggcggct ggctggtcga tgcgttttcg
    16741 tggcgggcga ttttctttct gaatgtgccg gtcgcatgcg cgaccatcgc gctggcgatc
    16801 gtcgccgtgc cggatagccg gcaggaggac gacacactgg acctcgaccc tgtgcttaag
    16861 gcgagcgcgc agccgaacct cgatcccgcg cttaaggcga gcgcgcaacc gaagctcgat
    16921 cccgcgctca aagcgagccc gcaaccgaag ctcgactggt tgggcgccgt cacagcggcc
    16981 gcggggctgg ccgcgttgac ctacggcctg accatcgcgt cggcgcgtgg cttcggcgac
    17041 cgcctggtgc tgacgtcgat cctcggcggc ctgctggtgc tgagcggatt cgtggcgctc
    17101 gaagcaaaga cccgcaatcc gatgatgccg ctcgacgtgt tccgttcgcg cgacttcgtc
    17161 ggcgcgaatc tcgtcacgct cttgctctac ttcgggctag gcggcgcgct gtttttcctg
    17221 ccgttcacgc tgatccgcgc gtatggctat agcgctaccg aggcaggcgc ggcattgctg
    17281 ccggtgccgg tcacgatcgg cctgttgtcg cgctttaccg gcggcctgac gagccgttac
    17341 ggcgcgcggg ccttgctagg caccggcccg gtgatcgcgg cgctcggctt cgcgatgctc
    17401 gcgctgccct gggtgcgcgg cgaatattgg cgcggctttt ttccggcgct tatcgtgctc
    17461 ggacttggga tgacgatcac cgtcgcaccg ttgacgacga cggtaatgtc ctccgtagca
    17521 gccgcgcgta cgggcgtcgc ttcggggatc aataatgccg tcgcccgggt ggcgagtctg
    17581 ctcgcgattg cggtgctcgg cattgtgttc gtgcggtcgc acgacgcggc gctcgatgcg
    17641 cggctcgatg cgttgcatgt cccgcaagcg gcgcgtcagg cggcacggct gacgccagac
    17701 tcgatggata cgagtccggc ggcggcaggg gcttcggttg cttcggttgc ttcggttgct
    17761 tcggttgctt cgggggcttc gggggcttcg ggtgcttcgg gggcggcggc cgtggcggca
    17821 cgggctgaag tgacgcacgc gcaagctgag gctctcggcg cggcattgca agcggtggcg
    17881 ctggcatcgg cgggatgcgc gctggccggc gcggcgctgg ccgccttgac gatccgcgca
    17941 gcgaccggaa aaacagccgt gccgcagcgc taaagggcgt gcatcaaggc gcgacgccgc
    18001 ttgcgccacc gccacgtgcg cggctttcgt ggccagcgaa cgcggcgctg cgtgcacaac
    18061 cacagttcag aaacggatga aacgtcccga gcgaacggtg gccatactgg gtggtactcg
    18121 gaggttcgag tctccgagcc aactgggagc ccgcacatga cgattacctg ctttatccgt
    18181 taccagatcg acccatttca acgcgatgca ttcgacgagt acgcgcgcaa ctggggacgc
    18241 atcattccgc gctgcggcgg tcatctgatc ggttattttt tgcctcatga agggaccgac
    18301 gacatcgcct ggggcttgat cgcattcaac agcctcgctg cctatgaagc ctatcgggcg
    18361 accctgcgag ccgaccccga agcgcgggag aatttcaggt tcgcgcaaac gaagcgcttc
    18421 attctgcgcg aggagcgcac cttcaccgag gtggtcgacg gcacgctcgg ggtggcggca
    18481 atgggggcga tgtaaaggag cgcgaacgga atcccgtgcg cagcagctgg agttaccggt
    18541 ccgcgcggtc tgccaggcaa gctttctgct tccggacgtt acaatttgct ccggttcgcc
    18601 gatcgcttgc gtaagcgctg gaagccgtgg aaggaacttg tacgaccaga atggtgtcgg
    18661 ttaagggtca caccgccgca ccgatccgcg cgttccggca ccgctgtccg caccttggta
    18721 ccccgcctta caccctatgc gcaaaatact ccacgcctgc tttctcgttc ttcttatcct
    18781 cggcgcctcg ccggtctttg ccgccccggc gcaaccctcc gccgcatccg cgccatcccg
    18841 cgccgcggtc acactgacgc ccgagcaggc ccgccaggcc ctcagcgtcc tcaatgaccc
    18901 caagcgccgc gcgcaagtca gcgacacgct ccaggcgatc gccgccgcag gcgcgctcag
    18961 cgcgccgccc gcctcggccg cggcggctgc gtcggcaccc gccgcggcca gcagcgccgc
    19021 cacgctcgtt ccagccgcgt tccagtccaa tggcctcgcc tcgcagctgg cccgtcaggg
    19081 cgcgcgttgg gccgcacacc tgggtgtgtc gttgcgcggc tcggtcgccg cgttgttcga
    19141 cgtcgcttcg gtgcgcgcct ggtggaattg gcagttcacc aacccacacg cgcgtagcgt
    19201 gctcgctcat gtcgcgttgt cgctgctggc ggcactgctg ccggcgctcg tgctcgaatg
    19261 gctcgcgcga cgcttgttgc gccgcgcata tgcggttctt gccgcgcgcc gcgaactgcg
    19321 cgatgaagac agcgcggcgc aagccgacct gcccgtcgac gtcctgccgc agccggccaa
    19381 tgcgccgaat gccgccccgc cgctgccggt ctctccgggc atggccgctt cggccgattc
    19441 acccacgtca cctgcttcgt ccacttcatc cgtttcacct gcttcgcccg cttcccctgc
    19501 tgcatccgct tcatccgttt cgtccgatgc aacggcccag acggcgaagc ccggcgcgtc
    19561 ctccgccgcg gccacctcca ccaccggcaa agccactgaa gtcaaaggca agcagagcgc
    19621 cgtgcatcac tggacgctcc tgcagcgtct gccgcgcgcg ctgctgacgc tggtgctgcg
    19681 cctgttgcca ctagcggtat tcgtcggcgc gacgagcgcg ctgatgtcga tctttaccga
    19741 cgacggcacg ccgcaagacc gcgcgctcga ttcgctgatc gacatctatg tactgtgccg
    19801 cgtgatcgtg atcgtcagcg gctttttcct gcagccgagc gcaccgcgcc tgcgtctgtt
    19861 gcgcatgagc gatgcatggg cgactttcgt gcagcgctgg atggtgcgga tcgtcgccgt
    19921 ggtcggcgtc ggttccgcga tcgcggagat cgcgcagtcg ctcggtttga acgacgccgc
    19981 gcatctcgcg ctgatgaagg tggtcgcgct cgccggccac atcatgatct cgatcctgat
    20041 cctgcaatgc gcgagtccgg tcggcgaatt gatccgcagg cgtttcgccg cgcgtgaatc
    20101 gctgcagatg ctcggcaatg cgctcgccga cgcgtgggcg tggttcgcgg tgttcatcgt
    20161 gatggcgctg tggttcgtct gggcgctcga cgtgcagaac ggctatcaca ccctcatgca
    20221 tctcggtggc ataacgctcg cgatcctggt cggcgcgcgg gtcgcctcga tcgtgatatt
    20281 gggtgcgatg gcgcggctct tccatgccca ggacgacgcg gaaaagtcgc tcgcgcatcg
    20341 gcatgcctac cgctactatc cgctgttgcg gcggatggtc gggtggatca ttggcatcgt
    20401 caccgcgctt gcgctgttgc aggtatgggg tgtcgacgtg gtcgacctgt tccgcagcgg
    20461 ttcggttgcc catcggctgg cttcagcgat cgtcacgatc ggcgtcgccg ccgtgatcgc
    20521 gttggtggtg tgggaaagtg tcaacgtctc tgtcgaacgg cggctggacc gctggaccac
    20581 cagcggcgac ctggtgcgcg ccgcgcgcct gcgcacgctg ctgccgatgt tgcgcagcgc
    20641 gctcttctgc gtgatcgctc tggtggtggt gctcaccggc ctgagcgaac tcggcgtgaa
    20701 cgtcgggccg ctgctggctg gcgcgagtat tttcggcgtg gcgctcggct tcggctcgca
    20761 aaagctggtg caggatttca tcaccggcat tttcctgttg atggaaaacg ccatgcaggt
    20821 cggcgactgg gtcacgctcg cgggcgtgtc cggcacggtc gaatatctgt cgatccgcac
    20881 ggtgcggctg cggggcagcg acggctcgct ttacaccgtg ccgttcagtt cggtctcgac
    20941 ggtcaacaac accaatcgcg ggctcggaaa tgcggcggtg aaggtgaaca tcgtgttcgg
    21001 ccaggacgtc gaccgggcga tcgacacgct caaggaaatc ggcgctgcgc tgcgcgagga
    21061 cgacaagttc aaggccggca tcctgtccga cttcgccttc tggggcgtcg atgccgtgga
    21121 cg
//
LOCUS       DATASH010000877         4006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2326884,
            whole genome shotgun sequence.
ACCESSION   DATASH010000877 DATASH010000000
VERSION     DATASH010000877.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2326884"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..626
                     /locus_tag="VHV99_23505"
                     /pseudo
     CDS             4..626
                     /locus_tag="VHV99_23505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012355663.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
     gene            705..3044
                     /locus_tag="VHV99_23510"
     CDS             705..3044
                     /locus_tag="VHV99_23510"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014251478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX3638389.1"
                     /translation="MSVPSVLTANPAAGAVASTTAELAIGGMTCASCVRRVEKALAKV
                     PGVGQVSVNLATEKATVQAGTAVSREQLVAAVTKAGYEATFIEPEAAPVAAPAHTGEL
                     VAVIVSALLSLPLVLPMLGLDLHLGVWVSLALASIVQFVLGARFYVGGFKAARSGEGN
                     MDLLVALGTSAAWGLSVYELFAHPGMSAHLYFEAAAVVITLVRFGKWLEARAKRQTTD
                     AIRALDALRPERARIRDAATGTERDVALADVRNGDMAIIRPGERLPVDGLIREGRTHV
                     DESLITGESLPVAKEIGARVTAGSINGEGVIAVETTATGAETTLARIIRLVESAQAAK
                     APIQRLVDRVSGIFVPVILVIALVTLVGWLLMGAGVETAVLNAVAVLVIACPCALGLA
                     TPAAIMAGTGVAARHGVLIKDPQALEMAHRIRIVAFDKTGTLTIGKPSMTVFEAAEGA
                     DRTEALGLAAAVQRLSDHPLARAVVAAADAERIEAVTVTATDAKAVAGRGVEARVGER
                     HLAIASDRWLDELQLTAPERLATRAAELEQLGNTVSWLVDLGGEGRSGAAPRMPDEPR
                     VLALLAFGDSLKPGARAAIERLKAMGIKSVLLTGDNAGSAAGVAKSLGIAPDAVHAHM
                     LPADKARVIAELKASAQGAVAMAGDGINDAPALAAADIGIAMATGTDVAMEAAGITLM
                     RGDPALVADAIDIARRTYRKIQQNLFWAFVYNLIGIPLAAFGLLNPMLAGAAMAFSSV
                     SVVTNALLLRTWRAQAGLTPVEAGATARGPDGKPITRPA"
     gene            complement(2966..3493)
                     /locus_tag="VHV99_23515"
     CDS             complement(2966..3493)
                     /locus_tag="VHV99_23515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX3638390.1"
                     /translation="MTHNRTIHRDTVRPEWVDYNGHLRDAFYMLIFSLATDAFMDRIG
                     LDDAARTSRGRSLFTLEAHINYLHEIKEGTAVRVEASVLAFDAKRVHLYMEMFAGEGT
                     GPIAASEQMLLHVDTTNGAKSATFDADIAGRIEAMLRDVPNEAPKEARYAGRVIGLPS
                     GPRAVAPASTGVKPA"
     gene            complement(3507..>4006)
                     /locus_tag="VHV99_23520"
     CDS             complement(3507..>4006)
                     /locus_tag="VHV99_23520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009975618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX3638391.1"
                     /translation="VGGEKTSEATLQAASAFYESIGMRALRVRKEVPGFIADRLLEAL
                     WREALHLVNEGVATTGEIDDAIRFGAGIRWSFMGTFLTYTLAGGEAGMRHFMQQFGPA
                     LELPWTKLVAPKLTDELIDRVVDGTTDQQGTHSIKQLERYRDDCITSVMTAIAEAKSR
                     HGIVD"
BASE COUNT          705 a         1231 c         1323 g          747 t
ORIGIN      
        1 ccgatctcca aggttttaca tcggctaatg gctgacaatc cgcttgcggc ccttgtcacg
       61 catggcgcaa acggctcgat gcgaaccacg tgccattcga gttcgacgcg agccagggtg
      121 aacacggcat tttgcgcgcg catgtggcgc gggcaaatcc aatttgtatg gagattgaga
      181 gcggctctgg cgcgctggtt attttccagg gcacgtccgg ttatatatcg ccaacttttt
      241 atccggccaa acaggaaacc catcggcaag tgccgactta taactatctg gtcgtgcacg
      301 cgcacggccg gatcgcgatc cgcgatgacg aggcattcgt gcgcggcgtg gtcgcgcgcc
      361 ttacccggaa aatggaagcc ggcgaagcgg tgccgtggaa aatgggcgat gccccgccgg
      421 atttcatcca gacaatgctg caagccgttg tcgggctcga aatcgaggtc acgcgcctcg
      481 tgggcaaggc gaagctgagt caggacgaaa cggcggccga ccgcatcggc gcgatcaacg
      541 gcgtgcgtca gcgcgaaacc gacgccgcga cagcgctcgc cgacgcaatg ttcaacgcac
      601 ttcctcctgt tgaaaataca ccttgacctt ccaatcattg gaatgtcgat actggtttca
      661 tcgagctgaa ttgaatggct cgcagtgaac cggaaaacct tgtcatgtcc gtaccgtctg
      721 ttttaactgc taatcctgct gctggcgctg ttgcgtccac taccgccgag ctcgctatcg
      781 gcgggatgac gtgcgcgtca tgcgtgcggc gcgtcgaaaa ggcgctggcg aaggtgccgg
      841 gcgtgggaca agtgtccgtc aacctggcga ccgaaaaagc gaccgtccaa gccggcacgg
      901 cggtttcgcg cgagcagctt gtcgccgccg tgaccaaggc tggctacgaa gcgacgttca
      961 tcgaaccgga agccgcgcct gtcgccgcgc ccgctcacac gggtgaactt gtagcggtga
     1021 tcgtgtcagc gctgctgagc ctgccgctgg tgctgcccat gctcgggctg gatctgcatc
     1081 ttggcgtctg ggtctcgctg gcgctcgcca gcatcgtgca attcgtgctc ggcgcgcgct
     1141 tttacgtcgg cggcttcaag gcggcacgtt ctggcgaagg caacatggac ttgctcgtgg
     1201 cgctcggaac ctcggctgcg tggggcttga gcgtgtacga gctgttcgcg catcccggca
     1261 tgtctgccca tctgtatttc gaggcagcgg cagtggtaat cacgctcgtg cgcttcggca
     1321 aatggctcga agcccgcgcc aagcggcaaa ccaccgacgc gattcgcgca ctcgacgcac
     1381 tgcgcccgga gcgcgcgcgg attcgcgatg cagcgaccgg gaccgaacgc gacgtcgcgc
     1441 tggcggatgt gcgcaacggg gatatggcaa tcatccggcc gggtgaaagg ctgccggtag
     1501 acggcctgat tcgcgaaggc cgcacgcacg tggatgaatc gctgattacc ggcgagagcc
     1561 tgcccgtggc gaaggaaatc ggggcacgcg tgacggcggg gtcgatcaac ggcgaaggag
     1621 tcatcgccgt ggaaacaacc gcgaccggcg ccgagaccac gcttgcccgg atcatccggc
     1681 tggttgaatc cgcacaggcc gcgaaggcgc ccattcagcg gctggtggac cgggtatcgg
     1741 ggatattcgt gccagtcatc ctggtgattg cactggtgac gctggtcggc tggctgttga
     1801 tgggcgcggg cgtggaaact gccgtgctga acgccgtcgc ggtgctcgtg atcgcgtgcc
     1861 cttgcgcgct cgggctggcg acacccgctg cgatcatggc cggaaccggc gtagcggcgc
     1921 ggcacggcgt gttgatcaag gacccgcaag cgctggaaat ggcgcatcgg atcaggattg
     1981 tcgccttcga taaaaccggc acgctgacta tcggcaagcc ttccatgacc gtgtttgagg
     2041 cggctgaagg cgcggaccgg acagaggctt tgggattggc ggcggccgtg cagcggctca
     2101 gcgatcaccc gctggcgcgc gcggtcgttg ctgccgccga cgccgagcgg attgaagccg
     2161 ttaccgttac cgcaaccgat gcgaaagcgg tggcggggcg cggcgtcgag gcgcgggtgg
     2221 gcgagcgtca tctggcgata gccagcgatc gctggctcga tgagttgcag ctcaccgcgc
     2281 cggaacggtt ggctacgcgg gcggcggaac tcgaacagct tgggaatacg gtgtcgtggc
     2341 tggtggatct gggcggggag ggccggtcag gcgctgcgcc acggatgccc gatgaaccgc
     2401 gcgtgctcgc cctgctcgcg ttcggcgact cgctgaaacc cggcgcgcgc gccgctatcg
     2461 aacggctgaa ggcaatgggc atcaagagcg tcctgctcac cggcgataac gcgggcagcg
     2521 cggccggcgt cgcgaaatcg ctcggcatcg cacccgacgc ggttcacgcg cacatgctcc
     2581 ccgccgacaa ggcgcgcgtg atcgcggaac tgaaggcatc ggcgcaaggc gcggtggcga
     2641 tggccggcga cggcatcaac gacgcgcccg cgctcgccgc cgccgatatc ggcatcgcga
     2701 tggcgaccgg caccgacgtc gccatggaag ccgccggcat cacgctgatg cgcggcgatc
     2761 cggccttggt ggcggatgcc atcgatatcg cgcggcgcac gtatcgcaag atccagcaga
     2821 acctgttctg ggcgtttgtg tacaacctga tcggcattcc gctcgccgcg ttcggactgc
     2881 tcaatccgat gctcgcaggc gcagccatgg cgttttccag cgtgagcgtg gtgacaaacg
     2941 cgttgctgct gcggacgtgg cgtgctcagg ctggcttgac gccggtcgaa gccggtgcca
     3001 ccgcccgcgg tcccgacggc aagccgatca cacggcccgc gtaacgggct tctttcggcg
     3061 cttcgttggg cacatcacgc aacatcgctt cgatgcggcc ggcgatatcc gcatcgaagg
     3121 ttgccgattt cgcgccgttg gttgtatcga cgtgaagcag catctgttcg ctcgccgcga
     3181 tgggcccggt tccctcgccc gcgaacatct ccatgtacaa gtggacgcgc ttggcatcga
     3241 acgcgagtac cgatgcctcc actcgaaccg ccgtgccttc cttgatctcg tgcagatagt
     3301 tgatgtgcgc ctcgagcgtg aacagcgaac ggccgcgcga ggtgcgggcg gcatcgtcca
     3361 gaccgatgcg gtccatgaac gcatcggtgg cgaggctgaa gatcagcatg tagaacgcgt
     3421 cacggagatg gccgttgtag tcgacccatt cgggcctcac cgtgtcgcga tggatcgttc
     3481 gattgtgcgt cactggtatg ccttctctag tccactatgc catgacggga tttcgcttcc
     3541 gctatggcgg tcatcacgct cgtgatgcag tcgtcgcggt agcgttccag ttgcttgatg
     3601 gagtgcgtgc cttgctgatc cgtcgtgcca tccaccaccc ggtcgatgag ttcgtccgtg
     3661 agttttggcg caaccagctt ggtccacggt aattctagcg ccgggccgaa ttgctgcatg
     3721 aaatgacgca tgccggcctc gccgcctgca agcgtatagg tcaggaatgt gcccatgaac
     3781 gaccagcgaa tgcctgcgcc aaaacgaatg gcgtcgtcga tttcgccggt ggtcgccaca
     3841 ccttcgttga ccaggtgaag cgcttcgcgc cagagcgctt ccagcaagcg gtccgcgata
     3901 aaaccgggaa cctctttgcg cactctcagc gcacgcatgc ctatcgattc ataaaatgcc
     3961 gatgctgctt gcaacgttgc ttccgaagtc ttctcgccgc cgacga
//
LOCUS       DATASH010000878         6351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3709118,
            whole genome shotgun sequence.
ACCESSION   DATASH010000878 DATASH010000000
VERSION     DATASH010000878.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6351
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3709118"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            462..857
                     /locus_tag="VHV99_23525"
     CDS             462..857
                     /locus_tag="VHV99_23525"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX3638392.1"
                     /translation="MHTISQDISTGRTRRTYSTSFKSELIEQCRQVGVSCSAVAISHG
                     MNPNVLRRWIKEAAHPSDAAATAPTPVDVELKPAFVALPMTVPTPQAPDAAQVNVRID
                     IQRNGTTVSVLWPHFCLSDSAAWVREVLR"
     gene            854..1189
                     /gene="tnpB"
                     /locus_tag="VHV99_23530"
     CDS             854..1189
                     /gene="tnpB"
                     /locus_tag="VHV99_23530"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEX3638393.1"
                     /translation="MIRIDAIWLATEPLDMRAGADTILTRVVKVFGSARPHHAYLFAN
                     RRSTRMKVLVYDGFGVWLAARRLNKGHFVWANGHSEIAETLNPEQLRALVIGLPWQTL
                     AHDHVIAVV"
     gene            1247..2587
                     /locus_tag="VHV99_23535"
                     /pseudo
     CDS             1247..2587
                     /locus_tag="VHV99_23535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006484960.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(2733..3737)
                     /locus_tag="VHV99_23540"
     CDS             complement(2733..3737)
                     /locus_tag="VHV99_23540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007749164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX3638394.1"
                     /translation="MRHSATQSFAVLVQKFFMQRLQQQQHASPCTVTAYRDSFRLLLD
                     FAHQRLHKRPIDITIEDLDAPFILDFLRYLEEDRRNSIRSRNARFAAIRSFMEYVSFE
                     EPSAMAIAQSILAIPMKRFEQPLVGFLSREHIEAILAAPDTGTWTGQRDHIMLATLYN
                     TGARVSELIDMRVSDLVLGPTSWVRIHGKGRKERSVPLWPDTAKDLKRWLRQYPRAPQ
                     EPLFPGRSGAPLTRIGFTDRLKLATQIASRQFPELVRRRIFPHLVRHSVAMHMLQSGV
                     PDTVIALWLGHESPATTHRYVEADLQMKERALKALQTPSRKPLRYRPKDDLLRFLEAL
                     "
     gene            complement(3734..4678)
                     /locus_tag="VHV99_23545"
     CDS             complement(3734..4678)
                     /locus_tag="VHV99_23545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012406572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX3638395.1"
                     /translation="MNPRTSFSTWIDDYLAVRRCAGFRLETEGMQLRQFARFAEQVGH
                     SGPLTVRLAVQWASSSHTGRRLTAARRIEVLRPFASYCRQFDPATEIPPRSLFGHAHR
                     RLTPHVFTGNEVLDLLAACDHLYPSGGLRGASCKTIFGLIAATGLRVSEATGLTRSDV
                     DLEQQLLQIRYGKFGKSRWVPIHPTTAEALRTYISKRDRDPLAGTTDAFFVFDCGRLA
                     STRSVERAFKVLRTALKWRVRGDHPAPRLQDLRHSFVCHRLQEWYARGLDVDRSILAL
                     STYLGHAKITDTYWYETATPELLAIAARRFVRCHGGAS"
     gene            complement(4675..5934)
                     /locus_tag="VHV99_23550"
     CDS             complement(4675..5934)
                     /locus_tag="VHV99_23550"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX3638396.1"
                     /translation="MSTYSRLKPGHILAQRWFARAALEAHANAYVQYLTERGYARETV
                     ERYFRSVAHFVRWISQQDVALCDLSETLVNRFLDGHLPRCRCAPRCRRARVDARAALK
                     HFLAMLGKAQTQLVPIVSASIAAELTDFERYLVEVRGLSNSTRTVRRRDVLEFLVDRF
                     GTSTVRISKLGPADVVRFVMRRTHGLMPGSVRSVVLSLRSYFLFKASCGTPTTPLIAA
                     LPRVALWRLAGLPEVLTPVEIGQLLNAFDRDSATGMRDFAITRCLLDLGLRRVEVAHL
                     RLDDIDWRAGVLRIRGKGKRTDVVPLPRLTGQAIAAYLEHGRPQTTRREVFVRHRPPL
                     NAAANVDIIRNAVRYAAERCGLQQRVRGTHIFRHTMACRMAQGGTPFKEIADLLRHRS
                     LNTTTIYAKVDLPALRRVALPWPGRRP"
     gene            6104..6301
                     /locus_tag="VHV99_23555"
                     /pseudo
     CDS             6104..6301
                     /locus_tag="VHV99_23555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013700242.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT         1347 a         1809 c         1934 g         1261 t
ORIGIN      
        1 gccgctgccg tcttgcatgg cgagttcggc cggagtggac tggacgcacc gctcgattcg
       61 aacgtgccga cgtggcccca gtcgcctaac gtgtcgctgg tgcctatccg agcatgtcga
      121 agcgatctgc tgccgagtcc gcgcgcatgc tcttcgcgta gctgctcccg cgagtcatga
      181 gcgcaccatc tgcgaccctg cgcccatacg gtcgttttcg cgcagtggct tgctgctccg
      241 caggatcggc cggccgccgc gtatcaggcg cgcgtcacgg cgactcagtc acacccggct
      301 aagtcggacg gtacctagac gaggccatct caaaaaaggg agatttgcgc acgccaagcg
      361 tctgcgacgt gcgcagttgg aatgtaagca tccggcgatc tcatctggtt ttctgttcct
      421 gggcgtaaga gcatacgttt ccacgatcag gaagcggaca cgtgcacact atttcccaag
      481 acatttcgac cgggcgcaca cggcggacct atagcacctc gtttaagtca gagcttatcg
      541 agcaatgtcg ccaggtcggc gtctcatgtt cagccgtggc gatctcgcat ggcatgaatc
      601 ccaatgtgct gcggcgttgg attaaagaag ccgctcatcc cagcgacgct gcggcgacgg
      661 cgcccacacc ggttgatgtc gagctcaagc cagccttcgt cgcgctgcca atgaccgtgc
      721 cgacaccaca agcgcccgat gcggcgcaag tgaacgtgcg aatcgacatt cagcgcaatg
      781 gcacaaccgt gtcggtgctg tggccgcact tttgtttgag cgacagcgca gcttgggttc
      841 gggaggtgct gcgatgatcc gcatcgacgc catttggctg gcgaccgaac ctctcgatat
      901 gcgtgccggc gccgatacca tccttacgcg tgtggtgaag gtatttggat ccgcacgacc
      961 tcaccatgcg tatctgtttg ctaaccggcg ttcgacacgc atgaaggtcc tggtgtacga
     1021 cggatttggc gtctggcttg ctgcacgacg cttgaacaag ggtcatttcg tttgggccaa
     1081 tggccatagt gagatcgctg agacattgaa tcccgagcaa ttgcgtgcac tcgtgatcgg
     1141 gctgccctgg cagacgttgg cgcacgacca cgtaatcgcg gtggtatagc aaatgacgta
     1201 aacagttttg attcccccag cctgtaggtg ctggcaaact tgatgcatga attcacccac
     1261 tgatctcgat gccctgaacc ccgatcaact gcgcgcactc gcgcggcagt tgatgaccca
     1321 ggtcgacgag aagacacgtg aatcacacta ccgccaaacg cgcattgacc agctcaccca
     1381 tgagctctcg gtcatcaaac gcctgcaatt cggcagacgt agcgaacagt tcacaaccga
     1441 gcagttgagc ttgctggacg aggccattga tgaggatttg gccgcgcttc aggtcgagct
     1501 ggatgaactg cgctcggacg cgccccagga gaagccccgc aagcaggcca aacgcttgcc
     1561 gttgccgccc cagctgcccc gtaccgacat ccatcacgag ccgacggagt tgagttgccc
     1621 ctgtggctgc cagcgcgtgc gtattggcga ggatgtcagc gagaaactcg actacgctcc
     1681 gggcgtgttc actgttgagc gccacatccg tgggaagtgg gcatgtaaag cgtgcgagac
     1741 gttgatccag gcgccggtgc cgccgcatgt gatcgacaaa ggaattccga ctaatggctt
     1801 gctcgctcag gtgttggtcg ccaagtatgg cgaccacctt ccactgtacc gacaggaaca
     1861 catcttcggc cgggcaggcc tagctattcc acaatcgacg ctcggcggct gggtgggcgt
     1921 atgcggtgtg cgtttgcagc ccttggtcga cgcgcttcgc gaggaactac tgcgccacgg
     1981 cgtcttgcat gcagacgaaa cgccggtgca gatgttgagc cccggcaaag ggaagacgca
     2041 tcgcgcctat ttatgggcgt acacgtcgac ccagttcgca gaggtgcgag cggtcgtgta
     2101 cgagttcgct gacggcagat ctggcgaaca tgcgcgcgcc ttcttgggca attggcgtgg
     2161 caagctggtc gttgacgatt acagcggtta taaagcagga tttaagctgg gcatcacgga
     2221 aattggctgc atggcacacg cacggagaaa gttcttcgat ctgcatgcga gcaagaagag
     2281 ccagatcgcc gagcaggcat tgaaattctt cgtggcgctc tacgacgtcg agcgcgcagc
     2341 cgccgactta gaccctgagc aacgacgagc gattcgtgag gagcgaggcc ggccaattgc
     2401 acaggcgttg cacgtatgga tgctcgcaca gcgcaaactt gtctccgagg gctcgccgat
     2461 cgccaaagca ctggactaca acctcaagcg ttggcaggcg ctcacgcgct atctcgacga
     2521 tcctcacgtg cccatcgata acaatcgaat cgagaatctg attagacctt gggccatcgg
     2581 acgggcttat cgtcggtttg cagttatcga aggttcgctg gaagtgtctc gagtggacaa
     2641 tcgacgatcg atgccggagc cctgtatttg caggacaacg gcatcgatgt ggatgcgtga
     2701 tccgtggtgc gccagctacg aactatccat aactagaggg cctccagaaa ccgaaggaga
     2761 tcatctttcg gccggtatcg aaggggcttg cgtgatggtg tctggagtgc cttcaatgcc
     2821 cgttctttca tctgcagatc cgcttcgaca tagcgatggg tggtagcggg actttcgtgt
     2881 ccaagccaca gcgcgatgac tgtatcggga acgcccgact gcagcatgtg catggcaacg
     2941 gaatggcgaa cgaggtgggg aaatatccga cgtcggacga gctctggaaa ctgtcgtgag
     3001 gcaatttggg tagctagttt caaccggtca gtgaaaccga ttcgtgtgag tggagcgcca
     3061 gatcgaccgg gaaatagcgg ttcctgaggg gcacgtggat attgccgtag ccagcgcttg
     3121 aggtctttgg cggtgtctgg ccacagcgga actgagcgct cctttcggcc cttgccatgg
     3181 atgcgaaccc atgaggtggg cccgagcaca agatcggaca cgcgcatatc gatgagttcc
     3241 gaaacacgag cgcctgtgtt gtaaagcgtt gccagcatga tgtggtcgcg ctgccccgtc
     3301 catgttccgg tatctggagc cgcgagaatc gcttcgatat gctcacggga cagaaatcct
     3361 accagtggtt gctcaaaccg cttcatggga atcgccagta ttgactgcgc tatcgccatg
     3421 gctgacggct cctcgaatga aacgtattcc atgaacgacc ggatggcggc aaaccgcgcg
     3481 ttccggctgc ggatggaatt gcgtcggtcc tcttcgaggt accggagaaa gtcgaggatg
     3541 aatggcgcgt ccaagtcttc gatggtgata tcgatgggcc gtttgtgcaa gcgttgatga
     3601 gcaaagtcca ggagtaaccg gaagctgtca cggtaggccg tcacagtgca agggctcgcg
     3661 tgttgttgct gttgcaagcg ctgcataaag aacttctgta ccaaaacggc aaaggactgg
     3721 gtggcggaat gtctcacgat gcacctccat ggcagcggac gaagcgccgg gctgcgatcg
     3781 ccagcagctc gggcgtagct gtttcatacc aatacgtatc cgtgatcttg gcgtgcccga
     3841 ggtaggtcga cagagcaaga atgctgcgat cgacgtccag cccgcgagcg taccactcct
     3901 gcaatctatg gcagacgaac gaatgtcgaa gatcttgcag ccgcggcgcg ggatggtccc
     3961 ctcgaacgcg ccacttcaag gctgtgcgca atactttgaa ggcacgctcg acgcttcgtg
     4021 tcgaggccag tcgaccgcaa tcgaacacga agaatgcgtc ggtggtacca gcgagaggat
     4081 cgcggtcgcg cttgctgatg taagtgcgca atgcttctgc cgtggtgggg tgtatcggca
     4141 cccagcgcga tttgccaaat ttgccatatc gaatttgtag cagctgttgc tcgagatcga
     4201 catccgaacg ggttagcccc gtcgcttccg agacgcgcag gcctgttgcc gcgatcaatc
     4261 caaagattgt cttacagctc gccccacgca ggccaccgga cggatataag tggtcgcagg
     4321 cagccaacag atcaaggacc tcgttgcctg taaaaacatg cggtgtcaac cgccgatggg
     4381 catgtccgaa caggcttcgc ggcggaatct cagttgcggg atcgaactgc cgacaataag
     4441 aggcaaaggg ccgaagcacc tcaatccggc gtgcagccgt tagccgtcgg ccagtgtgtg
     4501 aactgctggc ccactgaacg gcaagtcgta ccgtcagcgg accagaatgg ccaacctgtt
     4561 cggcgaagcg cgcaaattgc ctgagctgca tgccttcagt ttcaagtcga aagcctgcac
     4621 atcggcgcac ggccaggtaa tcgtcgatcc atgtcgagaa tgatgtacgt gggttcacgg
     4681 gcgcctccct ggccatggga gagccacgcg ccgaagcgcg ggaagatcga cctttgcgta
     4741 aatcgtcgtc gtgttgaggc tgcggtgacg caacaggtcg gcgatctcct tgaacggcgt
     4801 accgccttgt gccattcggc atgccatggt gtgtcggaat atatgcgtgc cgcgcacgcg
     4861 ctgctgcaga ccgcagcgtt cggcagcgta acgaacagcg tttcggatga tgtccacgtt
     4921 agccgcggca ttgagcggtg gccgatggcg gacgaagacc tcgcgccgtg tggtctgcgg
     4981 gcgtccgtgt tccagatacg ctgcgatcgc ctggccggtc aatcgtggaa gcggcacgac
     5041 gtcggttcgc ttgcccttgc cgcgaatcct cagcactcca gcgcgccagt cgatgtcatc
     5101 caggcgcagg tgcgcgactt ccactcgtcg caagcccagg tccagcaggc agcgcgtaat
     5161 tgcgaagtcg cgcatgcccg tggcgctgtc gcgatcgaaa gcgttgagca gttgcccgat
     5221 ctcgacaggc gtgagaacct cgggcagtcc ggcgaggcgc cacagggcga cccgtggcag
     5281 tgccgcgatg agtggtgtgg tgggtgttcc gcagctagcc ttaaacagga aatagctgcg
     5341 caacgagagg actacgctcc tgaccgaacc cggcatgagt ccatgcgtgc gacgcatgac
     5401 aaagcgaacg acatccgccg gaccgagctt cgaaatgcgt acagtgctcg tgccgaaacg
     5461 atcgacaaga aactcaagga cgtcgcgccg ccgaacggtg cgtgtgctat tggacagacc
     5521 gcggacttct acgagataac gctcgaaatc cgtcagttct gctgcaattg acgcggaaac
     5581 aatcgggacc agttgtgttt gagccttgcc gagcatggca agaaagtgct tcagcgcggc
     5641 gcgggcgtcg actctagcgc gacggcatcg tggggcgcat cggcaacggg gcagatgtcc
     5701 atcgagaaac cggttgacca gcgtctcgct gagatcgcaa agagcaacat cctgttgcga
     5761 gatccagcgg acgaagtggg cgacactcct gaagtagcgc tccactgtct ccctggcata
     5821 accgcgttca gtcagatatt ggacgtacgc atttgcatgc gcttccaatg cggctctggc
     5881 aaaccatcgt tgggcaagaa tatgcccggg tttgaggcgc gagtacgtgg acatggcaac
     5941 tctcctcgaa aacacgaaac agcatgagga aagtactcca agttatggaa gctcgccgga
     6001 ggtcggatac cactgaaaac aggtactatg tcccatatcc accgctcaac cttcgataac
     6061 tgcaaaccga cgataagatc atttatagaa aggattctat aaacgggcga actggttgtt
     6121 cgcgggttct ctacgcgctg gtcaacgagc ggccgccatc atgagtctgg tgcgttccgc
     6181 acaactcaac gggcacgacc cgtacgccta cctaaaagac gtcctcacgc gactgcccac
     6241 ccagcgagcc gaccagattg gggagttgct gccgcatcac tgggtaccag ctatcgcctg
     6301 atcgaagccg ccgatgactt taccgggtgc ttaccgtttt caggcgccgt g
//
LOCUS       DATASH010000879         6209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1535173,
            whole genome shotgun sequence.
ACCESSION   DATASH010000879 DATASH010000000
VERSION     DATASH010000879.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6209
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1535173"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VHV99_23560"
     CDS             <1..255
                     /locus_tag="VHV99_23560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638397.1"
                     /translation="PTTGLHPADVDRLMVQLQGLVDAGNTVVVVEHDMRVAAQSDWVV
                     DVGPGAGDAGGKIVASGTPAQVVRAKESRTAEYLRQHLVA"
     gene            complement(448..705)
                     /locus_tag="VHV99_23565"
                     /pseudo
     CDS             complement(448..705)
                     /locus_tag="VHV99_23565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006484960.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1388..2779)
                     /locus_tag="VHV99_23570"
     CDS             complement(1388..2779)
                     /locus_tag="VHV99_23570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX3638398.1"
                     /translation="MSSPTHHWIKRLAEIRKPAYLAIPDLIEEDLAAGRLRPRDRLPG
                     LRDLADELALNYTTVARAYAEAKKRGLLDSRAGSGTFVRGRTATLPLSGGGSIEMTMN
                     TPPEPADYAAHLRESAARLMANSDPYQLLRYHDFGGTPADKAAGTEWLKRRLAHCSPD
                     NVLVCPGIHSALVALVSQLARQSATICLDTLAYPGIKAIAAQLGVRLQALARDDEGPL
                     AHAFEALCKTDKPSAFYCNPTLQNPSTLTLTNKRREALADVALRYSVPIIEDDAYAML
                     PQLAPDTLASFAPELTYYITGMSKNLGAGLRVAYLHAPTPRQAQRLAGALRATTVMAS
                     PFTVSLATQWVADGTAQEMLSAIRSESKARQAIAARVLGASSYDAHPDGFHLWLPIPR
                     EGGWSASELALQLRSQGIAAVAAAAFSTDGNPPNALRVCLGGSKNRIECERALRGIAD
                     TLEQPHLLHSPVM"
     gene            3184..3666
                     /locus_tag="VHV99_23575"
     CDS             3184..3666
                     /locus_tag="VHV99_23575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_0572/Sll0783 family nitrogen starvation
                     response protein"
                     /protein_id="HEX3638399.1"
                     /translation="MPVVNKPLHQKGDYFVDYEEKVFEDVKAEPGEKALVTFHTVAFE
                     GSIGFVNLLQATRLQRKGFETSVLLYGPGVTLGLQRGFPTLGDEAFPGHLNFNKQLMK
                     FMEEGGKVYACRFALQALYGHGEASLIEGIRPISPLDVLDIQLLHRKDNALIIHTWTV
                     "
     gene            3685..4731
                     /locus_tag="VHV99_23580"
     CDS             3685..4731
                     /locus_tag="VHV99_23580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011880178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000257 - nitrilase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nit6803 family nitrilase"
                     /protein_id="HEX3638400.1"
                     /translation="MSEKRIVRAAAVQISPDFERSDGTLNKVCEAIDQAAREGVQLIV
                     FPETFVPYYPYFSFVRPPVASGADHMRLYEEAVVVPGPVTQAVAERARLHRMVVVLGV
                     NERDHGSLYNTQLIFDVDGQLLLKRRKITPTFHERMIWGQGDAAGLKVVQTGVARVGA
                     LACWEHYNPLARYALMTQHEEIHCSQFPGSLVGPIFAEQIEVTIRHHALESGCFVVNA
                     TGWLTEAQIASVTSDTNLQKALRGGCNTAIVSPEGQHLAAPLREGEGMVIADLDMALI
                     TKRKRMMDSVGHYARPELLSLAINERPATTVSPMPSPGNAWNASSSTDFNSTTGCRDE
                     FQRDPAGRKPAIDD"
     gene            4682..5794
                     /locus_tag="VHV99_23585"
     CDS             4682..5794
                     /locus_tag="VHV99_23585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_0568 family radical SAM protein"
                     /protein_id="HEX3638401.1"
                     /translation="MNSSETLPAASRQLMTELQSVGLRLADPGTGVASRRGGAGPSDH
                     KAVTVDGVTIMVPVHTNTAWHSPFVAAPADANGTSALLRGTIPIANISFPKVPRFYAM
                     QTLDGVPYSHIATLHSADVLATTVLQTCIRYESRRKTCKFCAIGQSLAAGRTIARKTP
                     EQLAEVARAAVLLDGVKHMVLTTGTPPTSDRGAQILCESAFAIRAAVDLPIQAQCEPP
                     DDDQWFARMKASGIDTLGMHLEVVTPSLRERIMPGKSGVPLSRYMEAFKAAVAVFGRG
                     QVSTYILAGLGDTAEAILAISRELIDLGVYPFVVPFVPISGTPLEDHPAPSPEFMKSV
                     LQPLGSMLAAAEMRSADIKAGCGKCGACSSLSSYEG"
     gene            5797..>6209
                     /locus_tag="VHV99_23590"
     CDS             5797..>6209
                     /locus_tag="VHV99_23590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_0567/Sll0786 family nitrogen starvation
                     N-acetyltransferase"
                     /protein_id="HEX3638402.1"
                     /translation="MFGEAIAGAPGELNDLDVAPAFYTPTEYRIKWTTLQWEADEAYK
                     LRRAVFCIEQGIFVGDDRDEIDERAQQLVAVSCMAGMPEQVVGTVRIHQEEPGVWFGS
                     RLAVHAAFRRHGKIGATLIRLAVTSAHALGCQTFLA"
BASE COUNT         1156 a         1865 c         1999 g         1189 t
ORIGIN      
        1 ccgaccaccg gcctgcatcc cgccgacgtc gaccggctga tggtgcaact ccagggactc
       61 gtggatgccg gcaacaccgt cgtcgtggtg gagcacgaca tgcgggtggc cgcacaaagc
      121 gactgggtcg tcgacgtcgg cccgggcgcc ggcgacgctg gcggcaagat cgtggcgagc
      181 ggcacaccgg cccaggtcgt gcgcgcgaag gaaagccgca ccgccgagta tctgcggcag
      241 cacctcgtag catgaaagaa ggaagcgtgt acgctagcga gcgccgcgct tctctttcag
      301 gaagtcgatc agcgcgcgca gtttgagcgg catttgcgcg cggctcgccg tgtagagaga
      361 gaaaccaagg aagctcggac accatgcgtc gagtacgcgc acgaggcagc catcgtcgag
      421 ttggtcgcgt acgtagtcct cgatgacatg cagcagaccc tgctgcagga tttcctgatg
      481 cagtgcgtcg cccagcggct gtagttgcat gccgtaccta ccgacccatg cgcccaatct
      541 cgatcgcggt atcgcgagag ccgcccgtgc aaagatctgc tcctgacgtt acaggggaag
      601 gtgctcagcg tacctggcaa ccagcaccga cgccagcagt cccgtggtcg ggataccctt
      661 gtcgatgatg tgcgcgggca ccgccgcctg aatcagtgtt tcgcagttcc ccggtagcgg
      721 cggtttgtcg gtgccgctcc acgagcgatc gatctatggc gacaagtggg caacgaacgt
      781 cccagcgcaa acggccgcgt tggccgaatt attcacgcag ctcgttcgac aggttccgga
      841 gaattgcagc cgttccaacg gcagagttct ccgctcgctg aacggcagaa tgtggccgcg
      901 tgccgccgga tgcaattgca tatgtgatcg aacggcacct ctggctgttc atacagcttc
      961 tattgtgttg catcgaccgg ttgaatccgc aaggtacaac ggccgttcca ttttccggtt
     1021 cgtccggcag accatcggcc atacccgccg ttcgcgttcg atcaactacg gtcgtcgcga
     1081 gcggccgctt tcggaattca cgaacgggca acttttcgag ccgtggccgt cctttactga
     1141 acgcccgcga gcgcgtgata gtttgctgta acggacgttc gaacgccaat cttcggcgaa
     1201 tgcgctctga gaaactgggc ttgtccacat ccggacaatg acaaaggcgc gtcggtaaac
     1261 ggattggggg agtgatctta cgcgatacgt taccgcgggt cgaccgacag cgcatcgacc
     1321 cagacgagtc cggtcctggg tgtttcaatt cgcgccatct gatcgtatca gcgccaacca
     1381 gattacttca catcactggc gaatgcagca gatgcggttg ctcgagcgtg tcggcgattc
     1441 cgcgcagcgc ccgctcgcat tcgatccgat tcttcgagcc tcccaggcat acgcgcagcg
     1501 cgttaggcgg attgccgtcg gtcgagaacg ctgcggcggc gacagcggca atgccttgac
     1561 tacgcaactg cagcgccagt tccgaagcac tccagccgcc ttcgcgggga atcggcagcc
     1621 acagatgaaa gccgtctgga tgcgcgtcat aggacgacgc gccgagcacg cgagcggcaa
     1681 tcgcctggcg cgctttcgac tcgctgcgaa tcgcgctgag catctcttgc gccgtgccgt
     1741 cggcaaccca ctgcgtggcg agcgacaccg taaatggact cgccatcacg gtggtcgcgc
     1801 gcagtgcgcc tgccaggcgc tgcgcctgac gcggagtcgg cgcgtgcaga tacgcgacgc
     1861 gcaacccggc acccagattt ttcgacattc ccgttatgta gtaagtcagc tcaggcgcga
     1921 aactcgccag cgtgtccggc gctagctgcg gcaacatcgc ataggcgtca tcctcgatga
     1981 tcggcacgct gtagcgcaac gcgacgtcgg cgagcgcttc gcgcctttta ttcgtgagcg
     2041 tcagcgtgct cggattttgc agcgtcggat tgcagtagaa cgcgctaggc ttgtcggtct
     2101 tgcagagcgc ttcgaacgca tgcgcgagcg gtccctcgtc gtcgcgcgcg agtgcttgca
     2161 aacgcacgcc gagttgcgcg gcgatggcct tgatgcccgg ataggcgagc gtatcgagac
     2221 agattgttgc actctggcgt gccagctgcg agacgagtgc gacgagcgcg ctatgaatgc
     2281 ccggacacac cagtacgttg tccggcgagc aatgagcgag gcggcgcttt aaccactccg
     2341 tgcccgccgc cttgtcggcc ggcgtgccgc cgaaatcgtg ataacgcaac agttggtacg
     2401 ggtcgctatt cgccatcaga cgggcggcgg attcgcgcag gtgtgcggcg tagtcagcag
     2461 gctcgggggg cgtgttcatc gtcatttcga tgctgccgcc accgcttagc ggcagcgtcg
     2521 cggtgcggcc gcgcacgaag gtgccgctgc cggcgcgcga atcgagcagg ccgcgctttt
     2581 tcgcctcggc ataggcgcgc gccacggtgg tgtagttcag tgccagttcg tcggccagat
     2641 cgcgcaggcc cggaagtcgg tcgcgcgggc gcaggcggcc ggcggcgaga tcttcttcta
     2701 tcagatccgg gatcgcgaga taagccggtt tgcggatttc ggcaagacgt ttgatccagt
     2761 ggtgagtcgg gctgctcatg gtgttcatgg aaagagaaga ctattggaag agacgtcacg
     2821 tatgtccgaa tcgccattgg aaagcgatcg cataacgtag tcggattgat cggattgatc
     2881 agattgattg gacggggcgc atgagtccat tgtggtgaat caccgcacct taacggttca
     2941 tttacaccat cttgattgca tctttctggt gcgatcgacg cctgctcggc cgctattatt
     3001 ggctggggct cgctgtatgc aaccttcggg ccaaaaattt ttcggcacac ctcccgaatg
     3061 attgtctatt tgatcggcaa gtgatcgcat ttgtggcgac catcattggc acggccgttg
     3121 cattagtggg ctcggcgcaa acgtaaagcg gcgccgttca cgcaaatcac atcggagagt
     3181 gccatgccag ttgtcaacaa accgctgcat cagaagggcg attacttcgt cgactacgaa
     3241 gagaaagtct tcgaggacgt gaaggccgag ccgggtgaaa aagcgctcgt cacgtttcat
     3301 acggtggcgt tcgaaggctc gatcggcttc gtcaatcttt tgcaagccac gcgtcttcaa
     3361 cgcaagggct tcgaaacctc ggtgctgctc tatggcccgg gcgtgacgct tggcctgcag
     3421 cgcggtttcc cgacgctcgg cgacgaagcg tttccgggtc atctgaactt caacaagcaa
     3481 ctgatgaagt tcatggagga aggcggcaag gtgtatgcat gccgcttcgc cttgcaggcg
     3541 ctgtatgggc atggcgaagc gtcgctgatc gaaggcattc gtccgatcag tccgctcgac
     3601 gtgctcgaca tccagcttct gcatcgcaag gacaacgcgc tgatcatcca cacctggacg
     3661 gtctgagcac aggcggccac caccatgtct gaaaaacgca tcgttcgtgc cgccgcagtc
     3721 cagatttcac cggacttcga gcgcagcgac ggcacgctga acaaggtttg tgaggcgatc
     3781 gatcaggcgg cgcgcgaagg cgtgcaactg attgtgtttc ccgaaacctt cgtaccgtat
     3841 tacccgtact tttcgttcgt gcggccaccg gttgcctccg gcgccgacca catgcggctt
     3901 tacgaagaag cggtggtggt gcccggcccc gtgacgcagg cggtggccga gcgtgcccgc
     3961 ctgcatcgca tggtcgtcgt gctcggggtg aacgagcgcg atcacggcag cctgtacaac
     4021 acgcagctga tcttcgatgt ggatgggcaa ctgttgctca agcgccgcaa gatcacgccg
     4081 acgttccatg aacggatgat ctgggggcaa ggcgatgcgg cgggactgaa ggtcgtgcaa
     4141 accggcgtgg cacgcgtggg tgcgctggcc tgctgggagc actacaaccc gctcgcccgc
     4201 tacgcgttga tgacgcagca cgaagaaatt cactgcagcc agtttccggg ctcgctggtg
     4261 gggccgattt tcgccgagca gatcgaagtg acgatccgcc atcacgcgct cgagtcgggt
     4321 tgcttcgtcg tgaacgcaac ggggtggctg accgaagcgc agattgcatc tgtcacgagc
     4381 gatacgaatc tgcaaaaggc gctgcgtggc ggctgcaata cggcgatcgt gtcacctgag
     4441 gggcagcatc tggctgcgcc gctgcgtgaa ggcgaaggca tggtgatcgc cgatctcgac
     4501 atggcgctga tcaccaagcg caaacgcatg atggattcgg tcggccacta tgcgcggccc
     4561 gaattgctga gcctcgcgat caacgagcgg ccggctacga cagtgtcgcc gatgccgtcg
     4621 ccgggcaatg cgtggaacgc ctcgtccagc accgatttca actcaaccac gggatgccgc
     4681 gatgaattcc agcgagaccc tgccggccgc aagccggcaa ttgatgactg aattgcagtc
     4741 ggtcggcttg cggctggccg atcctggtac cggcgtcgcc agccggcgcg gtggcgcggg
     4801 accgtccgat cacaaagccg tcacggtgga cggcgtgacc atcatggtgc cggtgcatac
     4861 gaacacggcg tggcattcgc cgttcgtcgc cgcgcctgcc gatgcaaacg gcaccagcgc
     4921 attgctgcgc ggcacgattc cgattgccaa tatcagcttt ccgaaggtgc cgcgctttta
     4981 cgccatgcag acgctcgacg gcgtgccgta ctcgcacatc gcaacgctgc acagcgccga
     5041 tgtgctggcg accaccgtgc tgcaaacctg cattcgctat gagagccgcc gcaagacgtg
     5101 caagttttgc gcgatcggtc aatcgcttgc tgcgggtcgc acgattgcgc gcaagacacc
     5161 ggagcagctc gcagaagtcg cgcgtgcggc tgtgctgctg gacggtgtca agcatatggt
     5221 gctgacgacc ggcacgccgc ccaccagcga ccgtggcgcg cagattctct gcgagagtgc
     5281 gttcgcgatc agggccgcgg tcgatctccc gatccaggcg caatgcgaac caccggacga
     5341 cgatcaatgg ttcgcgcgca tgaaggcgag cggcatcgac acgctcggca tgcatctcga
     5401 agtggtgacg ccgtcgttgc gcgaacgcat catgccgggt aagtcgggcg tgccgctgtc
     5461 gcgctatatg gaagcgttca aggcggcggt cgccgtgttc ggacggggac aggtgagcac
     5521 ctatatcctc gcgggactcg gtgacaccgc cgaagcgatt ctcgcgattt cgcgcgagct
     5581 gatcgatctc ggcgtgtatc cgtttgtcgt gccattcgtg ccgatcagcg gcacgccgct
     5641 tgaagatcat ccggcgccgt cgccggagtt catgaagtcg gtgctgcagc cgctcggctc
     5701 aatgctcgct gcggctgaaa tgcgctcagc ggacatcaag gccggctgcg gcaagtgcgg
     5761 cgcgtgctcg tccctgtctt catacgaggg gtgatcatgt ttggcgaagc gattgcgggt
     5821 gcaccgggtg aactgaacga cctggatgtt gcgccggcgt tttacacgcc gaccgaatac
     5881 cggatcaagt ggaccacgct gcagtgggaa gccgatgaag cctacaagct gcggcgcgcg
     5941 gtgttctgca tcgagcaggg catcttcgtc ggcgacgacc gcgacgaaat cgacgagcgt
     6001 gcccagcaac tggtggcggt gagctgtatg gccgggatgc ccgagcaggt ggtcggcacg
     6061 gtgcggatcc atcaggaaga gcctggcgtc tggttcggtt cgcggctcgc cgtgcacgct
     6121 gcgtttcgcc ggcacggcaa gatcggtgca acgctgatcc gtctcgccgt gacgagcgcg
     6181 catgcgctcg gctgtcagac attcctcgc
//
LOCUS       DATASH010000880         3246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6677442,
            whole genome shotgun sequence.
ACCESSION   DATASH010000880 DATASH010000000
VERSION     DATASH010000880.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6677442"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..138)
                     /locus_tag="VHV99_23595"
     tRNA            complement(52..138)
                     /locus_tag="VHV99_23595"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(102..104),aa:Leu,seq:cag)
     gene            455..2974
                     /gene="rnr"
                     /locus_tag="VHV99_23600"
     CDS             455..2974
                     /gene="rnr"
                     /locus_tag="VHV99_23600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEX3638403.1"
                     /translation="MQREKIIDKPLSKFPYPIPSREEILGVLRTSEAPLAANDIAEAL
                     SIKRQEREGFFKRLGAMERDGQIRLDQRNLYQLTHPSNFVAGRVQGHRDGYGFLIRDD
                     GQDDLFLPTGEMQKVMHNDRVLARIVGYDRRGRPEGHVVEVTDRANKRVIGRLLNENG
                     ALIVAPEDKRIGHDILIQQNTKKARVGQVVVVELTDFPSRHSQPLGRVVEVLGDIDDP
                     GMEIEIAVRKYGVPHEFSQAALDEAAKLPDEVRPVDARHRIDLRDVPLVTIDGEDARD
                     FDDAVYCEPVQVGRGEGFRLIVAIADVSHYVHPKSGLDADAIERSTSVYFPRRVIPML
                     PEKLSNGLCSLNPHVDRCVLVCDMIVTARGEVKAYQFYPGVINSAARLTYTEVAAVLK
                     NTKGPEAARRAALLPQLQNLYGVYKSLFAARQKRGAIDFDTTETYIVCNAQGKIEQII
                     PRTRNDAHKLIEECMLAANVCAADFLKRNKHPGLFRVHAGPTAEKLENLRTFLRGMGL
                     TLAGGDKPHASDYAALMAQIRERPDAQMLQTMLLRSMQQAVYSPDNIGHFGLAYEAYA
                     HFTSPIRRYPDLLTHRAIYAILQGRKYQPEPPQGVELNTALSPRARAMQQADEEKHGA
                     RNRSKDVAIWEELGLHCSANERRADEASRDVEAWLKCYFMRDKLGEEYGGMVNGVTSF
                     GIFVQLDSLFIEGLVHVTELGSDYFQYDEIKNELRGERTGIRYRLSDRVRVQVSRVDL
                     DARKIDFRLVRDTPIKPPVNRPALADKASGENSGARVRSLPPLEGGAGGAAAGAGGRR
                     KKAAPAQTAAVKEARAARGAAKKHGGAPAKAVSKPQARKKR"
BASE COUNT          670 a         1002 c         1015 g          559 t
ORIGIN      
        1 aacctcgcac aaaaaagtaa agccgcccgg aggcggcttt acttttgaat ctggtgccca
       61 agagaggact cgaacctcca cagtgttgcc accgctagga cctgaaccta gtgcgtctac
      121 caatttcgcc acctgggcag gcgatgaaca gcaaagaacg ccattatagc gacagattca
      181 gggctgtcaa gggtttctca aaagtcgctt aaacgacgcc gcagcgggcg tgcaggcggc
      241 tcacagcgac tattgacggg ccgcgtggcg cgcaagacgg gtgttagaat gcctcgcagt
      301 agttcgttgg cacggtgcac acgcccgtcg gactcagact tgaacgagcc ggacccgagc
      361 agaatctcca gtgcaattgc agtgcaactc gcaagtgcaa cttaagcgca acccaaacgc
      421 ttcttaagcc gagccacatc gctgaccgac gtccatgcaa cgagaaaaaa tcatcgacaa
      481 gcccttgagc aaatttccgt atccgatccc aagccgcgaa gaaatcctcg gtgttctgcg
      541 cacgagtgaa gcgccgcttg ccgcgaacga catcgccgaa gccctgtcga tcaagcgcca
      601 ggagcgcgaa ggatttttca agcgcctggg cgccatggag cgcgacggcc agatccggct
      661 cgatcagcgc aatctctatc agctgaccca tccgtcgaac ttcgtcgcgg gccgcgtgca
      721 gggccatcgc gacggctacg gttttctcat tcgcgacgac gggcaggacg atctgttcct
      781 gcccaccggc gaaatgcaga aggtcatgca caacgatcgc gtgcttgcgc gcatcgtcgg
      841 ctatgaccgg cgtggcaggc cggaagggca tgtcgtcgaa gtcacggatc gcgccaacaa
      901 gcgcgtgatc gggcgtcttc tgaacgagaa tggcgcgctg atcgtcgcgc ctgaagacaa
      961 gcgtatcggt cacgacattc tgatccagca aaacaccaag aaggccaggg tcggccaggt
     1021 ggtggtggtc gagttgaccg attttccgag ccgtcattcg cagccgctcg gtcgcgtggt
     1081 cgaagtgctc ggcgatatcg acgacccggg catggaaatc gaaatcgcgg tgcgcaaata
     1141 cggcgtgccg cacgaattca gccaggccgc gctcgacgaa gccgcgaagc tgcccgacga
     1201 agtccgtccg gtcgacgcgc gccatcgcat cgatctgcgc gacgtgccgc tcgtcacaat
     1261 cgacggtgaa gacgcccgcg acttcgacga cgcggtttat tgcgagccgg ttcaggtcgg
     1321 tcgcggcgag ggcttccgcc tgattgtcgc gatcgcggac gtctcgcact atgtgcatcc
     1381 gaagagcggg ctcgacgcgg acgcgatcga gcgcagcacc tcggtgtact tcccgcgccg
     1441 ggtgattccg atgctgccgg agaagctgtc gaacggcctg tgttcgctga atccgcacgt
     1501 cgaccgttgc gtgctggtgt gcgacatgat cgtcaccgcg cgcggcgaag tgaaggcgta
     1561 tcagttctat cccggcgtga tcaattccgc cgcacgcctg acttataccg aagtggcggc
     1621 ggtgctgaaa aacaccaagg gtccggaagc cgcacgccgt gctgccttgc tgccgcaatt
     1681 gcagaatctg tacggcgtct acaagtcgct gttcgccgcg cgtcagaagc gcggcgcgat
     1741 cgacttcgat acgactgaga cgtacatcgt ctgcaatgcg cagggcaaga tcgagcagat
     1801 cattccgcgt acccgcaacg acgcgcacaa gctgatcgag gaatgcatgc tggccgcgaa
     1861 cgtttgcgcg gccgacttcc tgaagcgcaa caagcatccg ggtctgttcc gcgtgcacgc
     1921 cgggccgacc gccgagaagc tcgagaatct gcgcaccttc ctgcgtggca tgggcctgac
     1981 gctcgccggt ggtgacaagc cgcatgccag cgattacgcc gcgctgatgg cgcagatccg
     2041 cgagcggccc gacgcgcaga tgttgcaaac catgctgctg cgctcgatgc aacaggccgt
     2101 ctacagcccg gacaacatcg gccacttcgg tctcgcctat gaggcgtatg cgcacttcac
     2161 gagcccgatt cgccgctatc ccgacctgct tacgcaccgc gcgatctacg ccattttgca
     2221 aggccgcaag tatcagccgg agccgccgca gggcgtcgag ttgaatacgg cgctgtcgcc
     2281 gcgcgcccgc gccatgcaac aggccgacga ggaaaaacac ggcgcccgca atcggtcgaa
     2341 agacgtggcg atctgggaag agctcggtct gcattgctcg gccaacgaac gccgcgcgga
     2401 cgaagcctca cgcgacgtcg aagcctggct caagtgctac ttcatgcgcg acaagctcgg
     2461 tgaagagtac ggcggcatgg tgaacggcgt aacctcgttc ggcattttcg tgcagctcga
     2521 ttcgctcttc atcgaaggcc tcgtgcatgt caccgaactg ggctcggact acttccagta
     2581 cgacgagatc aagaacgagt tgcgcggcga gcgcactggc attcgttacc gcctgtcgga
     2641 tcgtgtgcgc gtgcaggtga gccgcgtcga tctggatgcc cgcaagatcg atttccgtct
     2701 cgtgcgcgat acgccgatca agccgccggt gaatcgtccg gcgctcgccg acaaggcatc
     2761 cggtgaaaac agcggcgcac gcgtgcgttc gttgccgccg ctggagggcg gtgcgggtgg
     2821 cgcggcggca ggcgcgggtg ggcggcgcaa gaaggctgcg ccggcgcaaa ccgcggccgt
     2881 caaggaagca cgcgcggcac gcggcgcggc gaagaagcac ggcggcgcgc cggccaaggc
     2941 ggtatcgaaa ccgcaggccc gcaagaagcg ctgaagtcgc atgacggctt gaaccgcgcg
     3001 catcgggtac gttcagacgt accagttgcg cgcggtcttc gtttcatcgt agtcaagtcg
     3061 agtactggac aagcgtcgaa caggtatcgc ggcaggtacg caagcagcgc aataagcagg
     3121 ttcgtattca tcacagcagc acgcgttcaa ggcgcgctca atcaaaggtt tgtcagtcat
     3181 gtcacgtctc aaggttctat acggtttcca tgcagtgacc gcgcgtatgc ggcacgacgc
     3241 gtcgac
//
LOCUS       DATASH010000881         2888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1338212,
            whole genome shotgun sequence.
ACCESSION   DATASH010000881 DATASH010000000
VERSION     DATASH010000881.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1338212"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VHV99_23605"
     CDS             <1..404
                     /locus_tag="VHV99_23605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX3638404.1"
                     /translation="ADDLATRVKGIGTKSVTNLEAAGLTINGSSTPPTGAKAAAKSSS
                     TASTKAAPAAAPAATTAAATPAPATPAEASGAKASKSKNKSKAAAADTAASAPATAAA
                     ASAPADPSGTKAPKKAKKSKAASAAAASGGA"
     gene            581..1009
                     /locus_tag="VHV99_23610"
     CDS             581..1009
                     /locus_tag="VHV99_23610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HEX3638405.1"
                     /translation="MSLLDTIGSLVGKSPEGGGQQALISAALEFVNSQPGGLNGLIDR
                     FKEKGVGDVVTSWVSNGENQPIAADALHSVLGSETVTNLAAKAGMQPDQVTGLLSQLL
                     PHVVNAATPEGEVPAEGKLNSTTVLGALGGLSALFGKGNA"
     gene            complement(1160..>2888)
                     /locus_tag="VHV99_23615"
     CDS             complement(1160..>2888)
                     /locus_tag="VHV99_23615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3638406.1"
                     /translation="GAMDAGNMLKPALSRGELHCVGATTLDEYRKYIEKDAALERRFQ
                     KVLVDEPSVEATIAIMRGLQEKYELHHGVDITDPAIVAAAELSHRYITDRFLPDKDID
                     LIDEAASKIKMEIDSKPEEMDRLDRRLIQLKIEREAVKKEKDEASQKRLQLIEEEIER
                     LNREYSDLEEIWTAEKAAVQGSAQLKEEIEKTRAEITRLQREGKLEKVAELQYGKLPG
                     LEAQLKEVTQAEAKEQSSPTRRRLLRTQVGAEEIAEVVSRSTGIPVSRMMQGEREKLL
                     QIEEKLHARVVGQDEAISAVADAIRRSRAGLSDPNRPYGSFLFLGPTGVGKTELCKAL
                     ASFLFDSEDHLIRIDMSEFMEKHSVARLIGAPPGYVGYEEGGYLTEAVRRKPYSVILL
                     DEIEKAHPDVFNVLLQVLDDGRMTDGQGRTVDFKNTVIVMTSNLGSQVIQAMVGEPQE
                     AVKDAVWEEVKLHFRPEFLNRIDDVVVFHALDRSNIQSIARIQLQRLHERLAKLDMQL
                     LVSDEALEHVGKVGYDPLFGARPLKRAIQQEIENPVAKLILAGKFGPKDVIPVEVEDG
                     KLVFDRVVH"
BASE COUNT          519 a          988 c          847 g          534 t
ORIGIN      
        1 atgccgatga tctcgccacc cgagtcaaag gtattggcac caaatccgtg accaatctcg
       61 aagcggccgg cctgacgatc aacggctcgt ccacaccgcc tacgggcgcc aaggcggccg
      121 cgaagtccag cagcaccgcc agcaccaagg cggccccggc ggccgctcct gcggcgacta
      181 ccgcggccgc gacacccgca cccgcgacgc ctgctgaagc atccggcgcc aaggcgtcga
      241 agtcgaagaa caagagcaag gccgcggcgg ccgacacagc ggcttcggca ccggccacgg
      301 cggccgctgc cagcgcgccg gctgacccat ccggcaccaa ggcaccgaag aaggcgaaga
      361 agagcaaggc cgcatcggct gcggcggcct ctggcggcgc gtaattcagg tagcgcgccg
      421 gcagcatggc ggcagtgctg gttgcgtgcg atgcgcgcgt gatgagcacc tgacaccgcc
      481 ccgctgccgc ccgcctgcct accaccggcg ccgcgcaacc gcgcggcgtt ttcaccccgc
      541 cggctcgtcg aacacgggtc ggcattcaag agagaccgtc atgagcctac tcgacactat
      601 cggttccctg gttggcaaat cgcctgaagg cggcggccag caagccctca tttcagccgc
      661 gctggaattc gtcaatagcc agccgggcgg tttgaacggc ctgattgacc gcttcaagga
      721 aaagggcgtc ggcgacgtcg tgacgtcgtg ggtcagcaac ggcgagaacc agcccatcgc
      781 agccgatgcg ctgcacagcg tgctcggctc ggagaccgtc accaacctcg ccgccaaggc
      841 cggcatgcaa ccggaccagg tcaccggtct gctgtcgcag cttctgccgc acgtcgtcaa
      901 cgcggcgacg cccgaaggcg aagtccccgc cgagggcaaa ctgaattcga cgaccgtgct
      961 gggcgcgctc ggcggcctgt cggcgctgtt cggcaagggc aacgcataag actcagcagg
     1021 cgcgtccccc ggcaccgcct gataaccggc aaaagcaggc gaaaaaaagc ggcaacgaag
     1081 tcaccttcgt tgccgctttt taacgtcggc gggaacggcg tgaaaaccgc cgctccctgc
     1141 tgcgccgaac gcgttccgct taatgcacga cgcggtcgaa caccagcttg ccgtcttcca
     1201 cttcgaccgg aatcacgtcc ttcggaccga acttgccggc cagaatcagc ttggcgaccg
     1261 ggttctcgat ctcctgctgg atggcccgct tcaacggccg cgcaccgaac agcggatcgt
     1321 agccgacctt gccgacgtgc tcgagcgcct cgtccgacac cagcagttgc atgtcgagct
     1381 tggcaagccg ctcatgcaga cgctgcaatt gaatccgcgc gatcgactgg atgttcgaac
     1441 ggtcgagcgc atggaacacc accacgtcgt cgatccggtt caggaactcg ggccggaagt
     1501 gcagcttcac ttcttcccag accgcgtcct tcaccgcttc ctgcggctcg ccgaccatcg
     1561 cctggatcac ctgcgaaccg aggttcgagg tcatcacgat caccgtgttc ttgaagtcca
     1621 cggtacgacc ctggccgtcg gtcatgcggc cgtcgtcgag cacttgcagc agcacgttga
     1681 acacgtccgg atgcgccttc tcgatttcgt cgagcaggat cacgctgtac ggcttgcggc
     1741 gcacggcttc ggtcaggtag ccgccttctt cataaccaac atagcccggc ggcgcgccga
     1801 tcaaacgcgc gacgctatgc ttctccatga attcgctcat gtcgatgcgg atcagatgat
     1861 cttccgagtc gaacaggaac gaagccagcg ccttgcacaa ctcggtcttg cccacaccgg
     1921 tcggcccgag gaacaggaac gagccatacg ggcggttcgg atcggacaga cccgcgcgcg
     1981 aacggcggat cgcatcggcc accgcgctga tcgcttcgtc ctgaccgacc acgcgggcgt
     2041 gcagcttttc ttcgatctgc agcagctttt cgcgctcgcc ctgcatcatg cgcgacaccg
     2101 gaataccggt ggagcgcgag acgacttccg cgatttcctc cgcgcccacc tgggtgcgca
     2161 atagacgccg acgggtcggg ctgctctgct ctttcgcctc ggcctgggtc acttccttca
     2221 actgcgcctc gagacccggc agcttgccgt actgcaactc ggcgaccttc tcgagcttgc
     2281 cttcacgctg caagcgcgtg atttccgcac gcgttttctc gatctcttcc ttcaactgcg
     2341 cgctgccctg caccgctgct ttttccgcgg tccagatttc ttcgaggtcc gaatattcac
     2401 gattcagccg ctcgatttct tcttcgatca gttgcagacg cttttgcgat gcttcgtctt
     2461 tttccttctt gacggcttcg cgctcgatct tcaactggat caaacgacgg tcgagccgat
     2521 ccatctcttc cggcttcgag tcgatttcca tcttgatctt cgaggcggct tcgtcgatca
     2581 gatcgatatc cttgtccggc aggaagcgat ccgtgatgta gcgatgcgac agttcggccg
     2641 cggcgacgat cgccgggtcg gtgatatcga cgccgtgatg cagttcgtac ttctcctgca
     2701 aaccgcgcat gatcgcgatg gtggcttcca ccgacggctc gtcgaccagc accttctgga
     2761 agcggcgttc gagcgcggcg tctttttcga tgtacttgcg atactcatcg agcgtggtgg
     2821 cgccgacgca atgcagttcg ccgcgcgaaa gcgccggctt gagcatattg cccgcgtcca
     2881 ttgcgcct
//
LOCUS       DATASH010000882         2941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6279847,
            whole genome shotgun sequence.
ACCESSION   DATASH010000882 DATASH010000000
VERSION     DATASH010000882.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6279847"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..697
                     /gene="fixJ"
                     /locus_tag="VHV99_23620"
     CDS             53..697
                     /gene="fixJ"
                     /locus_tag="VHV99_23620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen response regulator transcription factor
                     FixJ"
                     /protein_id="HEX3638407.1"
                     /translation="MNSPVTTQETVFVVDDDEAVRDSLRWLLEANGYRVQCFSSAEQF
                     IDAWQPHQHPGQIACLILDVRMSGMSGLELQERLIADNASLPIIFVTGHGDVPMAVST
                     MKKGAMDFIEKPFDEAELRKLVERMLDKARSESTSVQQQRAAAERLGKLTAREHQVLE
                     RIIAGRLNKQIADDLGISIKTVEAHRANIMEKLNVNTVADLLRLALSNKPQAAQ"
     gene            1315..2634
                     /locus_tag="VHV99_23625"
     CDS             1315..2634
                     /locus_tag="VHV99_23625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013341411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HEX3638408.1"
                     /translation="MKRSGARRSRAFLACAALLGTPLFAGFVMPGVVHAQQGAAKISN
                     QQLDSLTAPIALYPDALLAQVLMASTFPQEVEAAAAWSKANSKLQGDDAVKAVASEPW
                     DPSVQSLVAFPQVLATMSSKPDWVAQIGNAFLAQPNDVMDSVQRLRKQAHSAGNLQTN
                     AQQKVVVEQSTIQIVPANPQIVYVPTYNPTVVYGTWPYPAYPPVYVPPPPGYAVASGL
                     AAGLAFGAGVAVANSLWGGFNWNTHDVNVNVNRYNNINVNNRINANSSTTHWNRNANI
                     NNANINRNNVNNNLSGAQRDAYRGRDDARAQAQQTLQNRTGQQMSGTASQRVQGIHQG
                     GGANPNVQNRAQNLNRDNALRGAGDGNAARQDAQRGEASRAKLAGGQHPGGGFGANGG
                     GQQYHGGGGGGFGAHGGGMQHGGGQMGGGGGRLGGGGAGGHHFGGRR"
     gene            2655..>2941
                     /locus_tag="VHV99_23630"
     CDS             2655..>2941
                     /locus_tag="VHV99_23630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2950 family protein"
                     /protein_id="HEX3638409.1"
                     /translation="MMRFFSRAPSRAHLRTLATALGSAGAMLIAPVLLGVANVAHAQA
                     VYPSATDAADALVDALARNDEAGLRHVLGNDFHQFIPAAGIGEDDIYQFLGA"
BASE COUNT          571 a          991 c          919 g          460 t
ORIGIN      
        1 cgagcaacgg cgggtcagag gcgccgacac cacaaaccgt tacgggagag ctatgaacag
       61 cccagtcacc acacaggaaa ctgtctttgt cgtcgacgac gacgaggccg tgcgagattc
      121 gctgcgctgg ctgctggagg caaacggcta ccgcgtgcaa tgcttctcca gcgccgagca
      181 gttcatcgac gcatggcagc cgcatcagca ccctggccag atcgcatgcc tgatcctcga
      241 tgtgcggatg tccggcatga gcgggctgga gttgcaggaa cgtctgatcg ccgacaacgc
      301 gtcgctgccg atcatcttcg tgacgggcca cggcgacgtg ccaatggcag tgtcgacgat
      361 gaaaaaaggc gcgatggact tcatcgaaaa accgttcgac gaagccgagt tgcgcaagct
      421 cgtggagcgc atgctcgaca aggcgcgcag cgagagcacc agcgtgcagc agcagcgcgc
      481 cgccgccgag cgtctgggca agttgaccgc gcgggagcat caggtgcttg agcgcatcat
      541 cgcggggcgc ctgaacaagc agatcgccga cgacctgggc atcagcatca agacggtcga
      601 agcgcatcgc gcgaacatca tggaaaagct caacgtcaac acggtcgccg atctgctgcg
      661 actcgcgctg tcgaacaagc cgcaagcggc gcaataaaac gccgggcggt attcccgcca
      721 caagttatcg agtgatcaac ggccggacta catgaaagtc cggcccgtgt cacttcttcg
      781 ccggcccgct cgccgcgctt catctgcttc gtccatgcgt cgcggttgcc ggcttgctgc
      841 cgggctcgct ttcgcgtctg cttcaagggc cgctgctagt cccgctctgc ggcccggcaa
      901 caaaagcgaa ccgtgcccat cctcgatgtg tccgcacaca tcgctcaatc ccatggcccg
      961 gcaatcacgg cgctgctcgc aacacctcaa tcctctgacc agattgacaa gcccggccac
     1021 gcctcctaag ttatacagaa ctcctcaacg gaaaccgtgg atgcgcgatc aatgcggcga
     1081 tgcgatgcgg cacatgtctt gcgcaattgc gcgaagcgcc gcgagcgcac gcagaggtcg
     1141 tggctcgacg ttctccactt tgccacggca tcggcatggg catcgccccg caccgcattg
     1201 ccgcgccccc acatctgcct gccagtactc ctgttcgcct cgccgcttgt gctttccttg
     1261 atttcatcgt gacgtatcga cgtctcgctg caacccaaac gggaggattt catcgtgaaa
     1321 cgatccggag cacgccgctc gcgcgcgttt ctggcttgcg ccgccctgct gggcacgccg
     1381 ctgttcgccg gtttcgtcat gccgggcgtc gtccatgcgc agcaaggcgc cgcgaagatc
     1441 tcgaaccagc aactcgattc gctgaccgcg cccatcgcgc tatatcccga cgcgctgctc
     1501 gcgcaggtgc tgatggcctc gaccttcccg caggaggtgg aagccgcggc cgcctggtcc
     1561 aaagcgaact cgaaactgca aggcgacgac gccgtgaagg cggtcgccag cgagccgtgg
     1621 gacccgagcg tgcaatcgct ggtcgcgttc ccgcaggtgc tcgcgacgat gtcgtcgaag
     1681 ccggattggg tcgcgcaaat cggcaacgct tttcttgctc agcccaatga cgtgatggac
     1741 tccgtgcagc gcctgcgcaa gcaggcgcac tcggccggca atctgcagac gaacgcgcag
     1801 cagaaggtgg tggtcgagca gagcacgatc cagatcgtgc cggccaatcc gcagatcgtg
     1861 tacgtgccga cctacaaccc gacggtcgtc tacggtacgt ggccgtatcc cgcatacccg
     1921 ccggtgtatg taccgccgcc acccggttat gcggtcgcga gcgggctcgc cgctggcctt
     1981 gcgttcggcg ccggcgtcgc cgtggcgaat tcgctgtggg gcggcttcaa ttggaatacg
     2041 catgacgtca acgtcaatgt gaaccggtac aacaacatca acgtgaacaa ccggatcaac
     2101 gcgaacagca gcacgacgca ctggaaccgc aacgccaaca tcaataacgc gaacatcaac
     2161 cgcaacaacg tcaacaacaa cctcagcggc gcgcagcgcg atgcgtatcg cggccgcgac
     2221 gacgcccgcg cgcaagcgca gcagaccctg cagaaccgca ccggccagca gatgagcggc
     2281 accgcgagcc agcgcgtgca aggaattcat caaggcggcg gcgcgaaccc gaatgtgcaa
     2341 aaccgcgcgc agaatctcaa tcgcgacaac gcgctgcggg gcgccggcga cggcaatgcc
     2401 gcgcgccagg acgctcaacg cggcgaagcg agccgggcca aacttgcggg cggccagcat
     2461 ccaggcggcg gctttggcgc gaacggcggc ggccagcaat atcacggtgg cgggggcggc
     2521 ggattcggcg cgcatggcgg cggcatgcag cacggcggcg gccagatggg tggcggaggc
     2581 ggcagactgg gcggtggtgg cgcgggtggg catcactttg gcggtcgccg ttgaacgttc
     2641 aacaggagtc tctgatgatg cgcttcttct cacgggcccc atcgcgcgcc cacctgcgga
     2701 cactcgccac cgccctcggc agcgccggcg ccatgctgat cgcgccggtg ctgctgggcg
     2761 tcgcgaacgt cgcccatgca caagccgtct acccgagcgc caccgacgcc gccgacgccc
     2821 tggtcgatgc cctcgcacgc aacgacgagg ccgggttgag gcatgtgctc ggcaacgact
     2881 ttcaccagtt catccccgcc gcgggcatcg gcgaggacga catctaccag ttcctcggcg
     2941 c
//
LOCUS       DATASH010000883         3687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5491793,
            whole genome shotgun sequence.
ACCESSION   DATASH010000883 DATASH010000000
VERSION     DATASH010000883.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5491793"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            449..1612
                     /locus_tag="VHV99_23635"
     CDS             449..1612
                     /locus_tag="VHV99_23635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX3638410.1"
                     /translation="MRRTSFLVRFILIGILLHIYVGFRLLPDMPIGGAARWACALWLV
                     LSIFLIPLGMLARTIKQQPLSDRLAWVGLLAMGFFSSLLVLTFARDLALASLLTVDAI
                     WPNTIAIAHWRTGSAAAVPLLALLSTLVGLFNARRRAKVVTIDIPIDDLPAALDGFTI
                     VQISDIHVGPTIKGRYVDAIVDAVNRLKPDLIAVTGDVVDGSVPQLTRHTQPLSRLSA
                     RHGAFLVTGNHEYYAGANAWIDEFRRLGLTVLLNEHVVVEHDGARAVIAGVTDFSAGH
                     HDPLHRSDPIAALAGAPGDVLIKVLLAHQPRSAEAAAAAGFTLQLSGHTHGGQFFPWN
                     FFVRFQQPFTAGLARLNGLWVYTSRGTGYWGPPKRLGAPSEITRLRLVPGEPD"
     gene            complement(1746..2267)
                     /locus_tag="VHV99_23640"
     CDS             complement(1746..2267)
                     /locus_tag="VHV99_23640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014973225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638411.1"
                     /translation="MKNKAPGIIAGALIAAAPFVSFAAGLPAPFEGSSTLQADGVVKF
                     VDQTKHSVTVLDAQGGEASFNITETGNLAQIRQGAKVHIRMMRNAMVSVTRGADGHTA
                     AQPVQQNTAQDVTAEVQAIDHASGIMALKSPNGAVFHIQGREPAKVAGVTPGMQVSVA
                     YAPQVSVAVAPAQ"
     gene            2930..3637
                     /locus_tag="VHV99_23645"
     CDS             2930..3637
                     /locus_tag="VHV99_23645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006396758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEX3638412.1"
                     /translation="MTIEFVWRLFAAFSCGVAIGLERQMRQRNAGLRTITLVASGACL
                     FVTLGVLTGNGTAGITQIAAYVVSGVGFLGGGVIMRDKGSIQGINTAATLWCSAAVGV
                     LCGAGHYGPALAGTAVVLLTNTVLREVSRTINATPVSNADLVREYVLTIVCREADEIH
                     IRTAVSNSMYSTPLSFQSLTSEDVEGDTGRIRVTATLKLHPKDQPKLEQMASRISMEK
                     SVSSVSWTAREAEPTPE"
BASE COUNT          674 a         1159 c         1083 g          771 t
ORIGIN      
        1 gtgatgtgcg tgatgtgcgt gatgcgcgtg atgtccgtgg cggacgtgat tgcctgatcc
       61 aagcagtttc atgatgcctc tcctgcctcg ccctctaccg gcgacaaagc gaaccatcgc
      121 gaaccatcgc aaggccgcaa cgaccctcag ccaggcgggt tctatttgag aatagtccac
      181 gctgagcaca attcaaagcc catttaatta gcatgtcacg gccattcagc cgggctgcgg
      241 gacgggcgaa ttgctgcaat gcgacacccg cctgccgcgt caaacctgca cgtgctcggc
      301 aatccggtgt gagccaattt ggtgccgtcg gcacggggtt gcacatctcg gtagaatggt
      361 ccggctcgtc cgctattcca aggttgctgt ttgcgagccg ccgccgtctt ttcactcgag
      421 ccggcccctt cccgcctcta tcctctttat gcgccgcaca tcgtttcttg ttcgcttcat
      481 cctgattggc atcctgctgc atatctacgt cggctttcgt ctgcttcccg acatgccgat
      541 tggtggcgcc gcccgatggg cgtgcgcgtt gtggctggtg ctgtcgatct ttctgattcc
      601 gctcggcatg ctggcgcgca ccatcaagca gcaaccgctc agcgacaggc tcgcgtgggt
      661 cggcctgctg gcgatgggtt tcttctcgtc gctgctggtc ctgacattcg cgcgcgatct
      721 ggcgctcgct tcgctgctca ccgtcgatgc aatctggccg aacacgattg ccattgcgca
      781 ctggcgcacc ggctcagcgg cagcggtacc gttgcttgcg ctgctctcga cactcgttgg
      841 cctgttcaac gcacggcgcc gtgccaaggt cgtgacgatc gatataccga tcgacgattt
      901 gccggcggcg ctcgacggct tcacgatcgt ccagatcagc gatatccacg tcggcccgac
      961 tatcaaaggc cgttatgtgg acgcaatcgt cgacgcggtg aatcggttga agccagatct
     1021 gattgctgtc accggcgatg tggtggacgg cagcgtgccg caactgacga ggcatacgca
     1081 gccgctgtcg cggctcagtg cgcgccacgg cgcgttcctc gtgaccggca accacgagta
     1141 ttacgccggc gcgaacgcct ggatcgatga gttccgacgc ctcgggttga ccgttctgct
     1201 caacgagcat gtggtcgtcg aacatgacgg cgcgcgcgcc gtgatcgccg gagtaaccga
     1261 tttttcggca ggccatcatg acccgttgca tcgcagcgac cccatcgcgg ccctcgccgg
     1321 cgcgccaggc gacgtgctga tcaaagtact gctcgcgcac cagccccgct cagccgaagc
     1381 ggccgcagcg gcgggtttca cgctgcaact atccggccac acgcatggtg gccagttctt
     1441 cccgtggaat ttctttgtac gctttcagca accgttcacg gcaggtctcg cacggttgaa
     1501 tggcctatgg gtctacacga gtcgcggcac gggctattgg ggaccgccga agcgcctggg
     1561 ggctccctcg gaaatcacgc gattgcgtct cgtgcccggc gagcccgact gaagcgtcga
     1621 agcggacgcc caatatcgcc gcgccgaagt catatcggat tgcgcaataa aaagcgcgga
     1681 ggtccgcaaa ggacctccgc gcttcggcgc tgacgcgccg cttcagttga caaacgttac
     1741 tgtgattact gcgcgggcgc cacggccacg ctgacttgcg gcgcgtacgc caccgacact
     1801 tgcataccgg gcgtcacacc ggcgaccttt gctggctcgc gtccctgaat atgaaacacc
     1861 gcgccattcg ggctcttcaa ggccatgatg ccggaggcgt ggtcgatggc ctgaacttcg
     1921 gccgtcacgt cctgcgcggt gttctgctgt accggttgag cagccgtgtg gccgtcggca
     1981 ccgcgcgtca cgctgaccat ggcgttgcgc atcatccgga tgtgcacctt ggcaccttgc
     2041 ctgatctgcg ccagattgcc cgtttcggtg atgttgaacg aagcttcgcc gccctgtgcg
     2101 tcgagcaccg tcaccgaatg tttagtctga tcgacaaact tgacgacacc gtctgcctgc
     2161 agcgtgctgc tgccttcaaa cggcgcgggc agaccggcgg cgaacgacac gaacggagca
     2221 gcagcaatca atgcaccggc aatgatgcca ggagctttat ttttcataca ttcctcaatt
     2281 ggggtgaaca gcggaaaaaa gacgatgttg tgcggatcgg atacgcctgg aaggacgcca
     2341 ggacagcaga acgattgcca ggcgaaaccg tgagaagtga aatgcgaatc gagcgccaaa
     2401 ccgcacagca tgtttcaaag tgtgcgaaca gtgtgttttc cgggataaat atcgtcaata
     2461 ttcggagtcc gtatttatgc gaggaaaata ctgatcctgt cgcgggaaat tccgcaagca
     2521 tctgtacgtg gtttccgccg ccctgcctca tccccgcttg acttaacttt gcagagctgc
     2581 ctaaaattcg cccaattcat ttaaccggaa tcacgatctt ggacagtagc agtcgtacca
     2641 gtcgagcttc gattcgcccg ctcgcctgat cggcaagatt tccgcgccag cctttgcctg
     2701 tcgccgtctt gccttcaggc ccgacggtgc acgtcgttta tatcctcgta ctacccgtga
     2761 agtgccgtat ggcgcgatgc cttgcgcgtg ctccatgact tttgcgccgc gagccctggc
     2821 tcgcttgcct cgccacacct gagcgcgccc gcgttccccc tgcggacacg tggtgctgtc
     2881 acgcttgggg ttcacactgt gcgaacacgc gccctaacct ttcttaccga tgacaattga
     2941 attcgtctgg cggctctttg ccgccttcag ctgcggcgtc gcgatcggcc tcgaacggca
     3001 gatgcgccag cgcaacgcag gcctgcgcac catcacgctg gtcgcgagcg gcgcgtgcct
     3061 gttcgtcaca ctcggcgtgc tcaccggcaa cggcaccgcc ggcatcacgc agattgccgc
     3121 gtatgtcgtt tcaggcgtag gctttctcgg cggcggcgtg atcatgcgcg acaagggttc
     3181 catccaggga atcaacaccg ccgcgacctt atggtgctcc gcggcggtcg gcgtattgtg
     3241 cggtgcgggc cattacggac cggcgctcgc cggcaccgcc gtcgtgctgt tgaccaatac
     3301 ggtgctgcgt gaagtgagcc gtacgatcaa cgcgacaccc gtttcaaacg ccgacctggt
     3361 gcgtgagtat gttctgacga tcgtatgccg cgaggctgac gagatccaca ttcgtaccgc
     3421 ggtatccaac tcgatgtatt cgacaccgct gtcgttccaa agcctgacga gcgaagacgt
     3481 ggaaggtgat acgggacgca ttcgcgttac ggcgacccta aagctgcatc cgaaagatca
     3541 gccgaaactg gaacagatgg caagccgcat cagcatggaa aagagcgtat cgagcgtcag
     3601 ttggaccgcg agagaagcgg agccgacgcc ggagtgagtc gccacgcgta agctcccccc
     3661 atattcgcgc gcatcgtcta cacttag
//
LOCUS       DATASH010000884         2221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4896654,
            whole genome shotgun sequence.
ACCESSION   DATASH010000884 DATASH010000000
VERSION     DATASH010000884.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4896654"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            419..1021
                     /locus_tag="VHV99_23650"
     CDS             419..1021
                     /locus_tag="VHV99_23650"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3638413.1"
                     /translation="MLSCIKSTWPPRLVTVPGLHGSEGAHWQTWLERQFARSLRVEQA
                     DWDTPDVAAWAKSLRDLLARERGPFVLAAHSFGCLATAHALQQASYANDVVGVLFVAP
                     ASPEKFAFAGPFDARRLGVPSILIGSETDPWMPLAGSRDLAQRFGSAFVNLGDAGHIN
                     TAAGFGPWPRAKYFVDTLVHCAAPLRFREETLGLAQHAFV"
     gene            1108..2133
                     /locus_tag="VHV99_23655"
     CDS             1108..2133
                     /locus_tag="VHV99_23655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015001460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX3638414.1"
                     /translation="MAGKTGTSRWLAAGMTAITLALGGVPAAHADTSLLNVSYDVTRE
                     LYKDINAGFIAAYKAKSGETVSIRQSHGASSAQALSVLQGLQADVVTMNQPNDIDLLA
                     EKGQLLPANWRTRLPDDSVPYTTTMVFLVRKGNPKHIKDWDDLAKPGMQMVIANPKTS
                     GNGRYAYLAAWGYRKLHGGTDAQAIDFEKAIFRNVPVLDTGGRGATTTFTQRGIGDVL
                     VTFENEVSLIATGVGDGNFEAVYPSVSLLAAPPVALVDKVVDKRGTRKEAQAYLDYLW
                     SPAAQEIIAQHHLRPRDKNVLARHAAEFKPIKTFTVEEIFGSWQKAQQTHFSDGGTFD
                     QIIVDKK"
BASE COUNT          424 a          703 c          711 g          383 t
ORIGIN      
        1 tgcaacgccc gcggcgctgc agacgacgct tgcggcatgg accacctcgt tttatggata
       61 ggcttcattc tactttttcg agagcactcg cgcgcgtttg cgcacggacg tgcggtttgg
      121 gcggcaaagc tcgcttgccc ccaatcctca cctcacctcg gctcacctcg gcccgggcgc
      181 cccgcctcgc gccgactggc gggccgcggg caaggcaagg gccgcggtta cggcaacggc
      241 cacggttacg gcaacggcca cggttacggc gagggccatg gttacggcga cggccacggt
      301 cacggcaagg gccgctgcgc cgcgcattta ccccataaac aaagcacaaa aacttggttc
      361 gcccttgcga cgcccggcgg gacaatcact tcccggattc cggcagcgaa ggactgacat
      421 gctttcatgc atcaaatcga catggccgcc gcggctcgtc accgttcccg gcctgcacgg
      481 cagcgagggc gcgcactggc aaacatggct cgaacggcaa ttcgcgcgct cgctgcgcgt
      541 cgaacaggcc gattgggaca ccccggacgt cgccgcctgg gcgaaatcgc tgcgcgatct
      601 gctcgcgcgc gaacgtgggc cgttcgtgct ggccgcccac agcttcggct gcctggcgac
      661 tgcgcacgcg ttgcagcagg cctcgtacgc gaacgatgtg gtcggcgtgc tgttcgtcgc
      721 gcccgcgagc ccggagaaat tcgctttcgc cggcccgttc gacgcacggc gcctcggcgt
      781 gccgtcgatt ctgatcggca gcgaaaccga tccgtggatg ccgctcgccg gttcgcgcga
      841 tctcgcgcag cgtttcggca gcgcattcgt gaatctcggc gacgcgggac atatcaacac
      901 ggcggcggga ttcggcccat ggccgcgcgc caaatatttc gtcgacacgc tggtgcattg
      961 tgcggcgccg ctgcgctttc gcgaggagac gctcgggctc gcgcagcacg cattcgtatg
     1021 agcgatcaga aagcgatccg ctagaatccc gcttcacgct gcacgttcgt gcggccgcag
     1081 attttttcat cgataggcgg ggataagatg gcaggcaaga cggggacatc gcgctggctg
     1141 gcggccggca tgacggcaat aacgctcgcg ctcggcggcg taccggcagc gcacgcggac
     1201 acatcgctgc tcaacgtgtc gtacgacgtg acacgcgagt tgtacaagga catcaacgcc
     1261 ggcttcatcg cggcatacaa agcgaagagc ggcgagaccg tgtcgattcg ccagtcacat
     1321 ggcgcgtcga gcgcgcaagc gctgtccgtg ttgcaaggac tgcaggctga cgtcgtgacg
     1381 atgaaccagc cgaacgacat cgacctgctc gccgaaaagg gccagctctt gcccgccaac
     1441 tggcgcacgc gcctgccgga cgacagtgtt ccgtacacta cgacgatggt gttcctcgtg
     1501 cgcaagggca acccgaagca catcaaggac tgggacgatc tcgccaagcc cggcatgcaa
     1561 atggtcatcg ccaacccgaa gacctcgggt aatggccgct acgcctatct ggcggcatgg
     1621 ggttatcgca agctgcacgg tggcaccgat gcgcaggcga tcgacttcga gaaggcgatc
     1681 ttcaggaacg tgccggtgct ggacacaggc gggcgcggcg cgacaacgac cttcacgcaa
     1741 cgcggcattg gtgatgtgct ggtgacgttc gaaaacgaag tgtcgttgat cgccacgggc
     1801 gtcggcgacg gcaatttcga agcggtgtat ccgtcggtga gcttgctggc tgcgccgcca
     1861 gtggcgcttg tcgacaaggt ggtcgacaag cgcggcacgc gcaaggaagc gcaggcgtat
     1921 ctcgactatc tgtggtcgcc ggccgcgcag gaaatcatcg cacagcatca tctgcgtccg
     1981 cgtgacaaga atgtgctcgc caggcacgcg gctgagttca agccgatcaa gactttcacg
     2041 gtcgaggaga tcttcggcag ctggcagaaa gcgcagcaaa cgcatttctc tgatggcgga
     2101 acgttcgatc agatcatcgt cgacaagaag taaatcttgg cctcagaagt agaatggccg
     2161 cctcgtttcc gaacggcacc ccagatcgtt ggaccgatgt ccaacttcag ggagcagttc
     2221 t
//
LOCUS       DATASH010000885         3374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2721634,
            whole genome shotgun sequence.
ACCESSION   DATASH010000885 DATASH010000000
VERSION     DATASH010000885.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2721634"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VHV99_23660"
     CDS             complement(<1..274)
                     /locus_tag="VHV99_23660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006046728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003860 - 3-hydroxyisobutyryl-CoA
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX3638415.1"
                     /translation="MSALPGVALDTSVEAEREILFRVVNRVAIITLNRPAVLNALSHA
                     MVRELAVLVEHCRTDDGIVALVLKGAGTKGFCAGGDVREVYRLAKNG"
     gene            complement(271..1116)
                     /locus_tag="VHV99_23665"
     CDS             complement(271..1116)
                     /locus_tag="VHV99_23665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX3638416.1"
                     /translation="MIELDYAHHGAVALLTLKRPPANAFTPDGLLQLQQTVERLNGEA
                     RVRAIVITGDGPKFFSAGADLNTFADGNREVARTAATRFGAAFETLQNARPVVIAAIN
                     GYAMGGGLECALACDIRIAEQHASMALPETAVGLLPCGCGTQTLPWLIGEGWAKRMIL
                     TGERVDASTALRIGLVEEVVEKGAAREAALTMAARVATLSPQAVGFSKTLIHQGRNGV
                     PRAAALAVERERFVDLFDGADQREGVNAFLEKRAPRWQVTQAGQAEQAAHAEQVANVA
                     QETHR"
     gene            complement(1116..2018)
                     /gene="mmsB"
                     /locus_tag="VHV99_23670"
     CDS             complement(1116..2018)
                     /gene="mmsB"
                     /locus_tag="VHV99_23670"
                     /EC_number="1.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008442 - 3-hydroxyisobutyrate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyisobutyrate dehydrogenase"
                     /protein_id="HEX3638417.1"
                     /translation="MKIGFIGLGNMGAPMALNLLKAGHAVNVFDLNAQAVQALVDAGA
                     KAGGSPKAAVSDVECVVTMLPAAAHVRSVLTADDGVFAGIAKGVTIIDSSTIDPASVK
                     AFAELAAQHGNSFVDAPVSGGTGGAAAGTLTFMVGGSAAAYEQVKPVLASMGKNIVHC
                     GETGTGQVAKICNNLVLGITMAGVAEAMSLGEALGIDSKVLGGIINTSTGRCWSSDTY
                     NPMPGVIETAPSTRGYTGGFGTDLMLKDLGLATDAAKSARQPVYLGALAQQLYQTMST
                     KGAGRLDFSAVIKLYRQDGKDADA"
     gene            complement(2192..>3374)
                     /locus_tag="VHV99_23675"
     CDS             complement(2192..>3374)
                     /locus_tag="VHV99_23675"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX3638418.1"
                     /translation="GLEVVEHACSIGTLQQGEFAENVAGGVDTYTLRQPIGVCAGITP
                     FNFPAMIQLWMFPMAIVCGNTFVLKPSEQDPMSTMQLVELALEAGVPKGVLNVVHGGK
                     DVVDALCTHDLVKAISFVGSTAVGTHVYRLGSEHGKRVQSMMGAKNHAVVLPDANREQ
                     TLNALAGAGFGAAGQRCMATSVVVLVGAAQQWLPDLVAKAKTLKVNAGNEPNTDIGPV
                     VSRAAKQRILGLIETGVKEGATLALDGRDVTVPGYEQGNFIGPTVFTDVTTEMEIYRQ
                     EIFGPVLVVLNAATLDDAIALVNRNPFGNGVGLFTQSGAAARKFQSEIDIGQVGINIP
                     IPVPVPFFSFTGSRGSKLGDLGPYGKQVVQFYTQTKTVTARWFDDDTVNDGVNTTISL
                     R"
BASE COUNT          579 a         1152 c         1008 g          635 t
ORIGIN      
        1 cgccattctt cgcgagccgg tacacctcgc gcacgtcgcc gccggcgcaa aaacccttgg
       61 tgcccgcgcc tttcagcaca agcgcaacga tgccgtcatc ggtacggcaa tgttcgacca
      121 gcacggcaag ttcacgcacc atcgcatgcg atagcgcatt gagcaccgcc ggccggttca
      181 gcgtgatgat cgccacccgg ttgacgacgc gaaacagaat ttcgcgctcc gcttcgacgc
      241 tggtatcgag cgcgacgccg ggcagggcgc tcatcgatga gtctcctgcg cgacgtttgc
      301 aacctgctca gcgtgggctg cctgctccgc ctgtccagct tgcgtcacct gccagcgtgg
      361 cgcgcgtttc tcgagaaacg cattgacgcc ttcgcgctga tcggcgccgt cgaacagatc
      421 gacaaagcgt tcccgttcca ccgccagggc cgccgcacgt ggcacgccgt tacggccctg
      481 atggatcagc gtcttgctga aaccgaccgc ttgcggactc aacgttgcga cacgcgccgc
      541 catcgtcaac gcggcctcgc gcgcggcgcc cttctccacc acttcttcga cgagaccaat
      601 acgcaatgcg gtagacgcat cgacgcgttc gccggtcagg atcatccgct tggcccagcc
      661 ttcaccaatc agccacggca acgtttgcgt gccgcagccg catggcagca agcccaccgc
      721 cgtttcaggc aaggccatcg acgcgtgttg ctcggcgatg cggatatcgc aggccaacgc
      781 gcattcgagt ccaccgccca tcgcatagcc gttgatcgcc gcaatgacca ccggccgcgc
      841 gttctgcaag gtttcgaacg ccgcgccgaa gcgcgtcgcc gccgtgcgcg cgacttcgcg
      901 attgccgtca gcgaaggtgt tcaggtcggc gcccgcactg aagaatttgg ggccatcgcc
      961 cgtgatcacg atggcccgca cgcgcgcttc gccgttcaga cgctcgacgg tctgctgtaa
     1021 ctgcagcaga ccatccggtg tgaacgcgtt cgcgggcggt cgtttgagcg tcagcagcgc
     1081 gacggcgccg tggtgtgcgt agtcgagttc gatcatcacg cgtctgcgtc cttgccgtcc
     1141 tggcgataga gcttgatcac cgccgagaag tccaggcgtc ccgcgccctt cgtgctcatc
     1201 gtctgataaa gctgctgcgc gagcgcgccg agatacaccg gttgacgcgc ggatttcgcg
     1261 gcgtcggtgg cgaggcccag gtccttgagc atcagatccg tgccgaagcc accggtatag
     1321 ccgcgcgtgg acggcgcggt ttcgatcacg ccgggcatcg ggttatacgt gtccgaactc
     1381 cagcagcggc ccgtcgaggt attgatgatg ccgcccagca ctttcgagtc gatgccgagc
     1441 gcctcgccca gcgacatcgc ctcggccacg cccgccatcg tgatgccgag cacgaggttg
     1501 ttgcagatct tcgcgacctg gccggtgccg gtctcgccgc aatgcacgat gttcttgccc
     1561 atcgacgcca acaccggttt cacctgctcg taagccgccg cactgccgcc gaccatgaag
     1621 gtcagcgtgc ccgccgccgc gccgccggtg ccgcccgaga cgggcgcgtc gacgaagctg
     1681 ttgccgtgct gcgcggccag ttcggcgaac gccttcacgc ttgccgggtc gatggtgctc
     1741 gaatcgatga tcgtcacgcc ttttgcaatg ccggcgaaca cgccgtcgtc cgctgtcagc
     1801 acgctgcgca cgtgcgcggc agcgggcagc atcgtcacca cacactcgac gtcggacacc
     1861 gccgctttcg gcgagccccc ggctttagcc ccggcgtcga ccagcgcctg caccgcctgc
     1921 gcgttgaggt cgaatacatt gaccgcgtgg cccgccttga gcaggttgag cgccattggc
     1981 gcgcccatgt tgccgagtcc gataaagcct attttcatgg tgtcatctcc tgtcggtggg
     2041 agttagcgct ttagcgctta ctcccacccc atgcaaagct gtcggtggga gttagcgctt
     2101 tagcgcttac tgccacccca tgcaaagctg tcgatgggag ttagcgcttt agcgcttact
     2161 cccatcccat gcaaagcatc gaccttggaa atcagcgcag gctgatcgtc gtgttgacgc
     2221 cgtcgttgac ggtgtcgtcg tcgaaccagc gggcggtgac ggttttggtc tgcgtgtaga
     2281 actgcacgac ctgcttgcca tagggtccga ggtcgccgag tttcgagcca cgcgagccgg
     2341 taaaactgaa gaacggcacg ggcaccggaa tcggaatgtt gatgccgacc tggccgatat
     2401 cgatctcgct ctggaacttg cgcgccgccg cgccgctctg tgtgaagagg cccaccccgt
     2461 tgccgaacgg attgcggttg acgagcgcga tcgcgtcatc gagcgtcgcg gcattcagca
     2521 ccaccagcac cgggccgaag atttcctggc ggtagatctc catctcggtg gtgacatcgg
     2581 taaagaccgt cgggccaatg aagttgcctt gctcgtagcc cggcaccgtc acgtcgcggc
     2641 catcgagcgc cagcgtggcg ccctctttca cgcccgtttc gatcaggccg agaatgcgtt
     2701 gcttcgccgc gcgtgagacc accgggccaa tatccgtatt cggctcgttg cccgcgttga
     2761 ctttgagcgt cttcgccttc gcgaccagat cgggcagcca ttgctgcgcc gcgcccacca
     2821 gcacgaccac cgaggtcgcc atgcaacgct gtcccgctgc gccgaaaccg gcaccagcca
     2881 gcgcgttcaa ggtctgctca cgattcgcat cgggcagcac gactgcgtga ttcttcgcgc
     2941 ccatcatcga ttggacgcgt ttgccatgtt cgctgccgag gcgatagacg tgcgtgccga
     3001 ccgccgtcga gccgacgaac gaaatcgcct tcaccagatc gtgcgtgcag agcgcgtcga
     3061 ccacgtcctt gccgccgtgc acgacattca gcacgccctt cggcacaccg gcttcgagcg
     3121 ccagttcgac cagttgcatg gtcgacatcg gatcctgctc ggacggtttc agtacaaacg
     3181 tattgccgca gacgatcgcc atcggaaaca tccacaactg aatcatggcg gggaaattga
     3241 acggcgtgat gccggcgcat acgccgatcg gttgacgcaa cgtgtacgta tccacaccgc
     3301 ccgccacgtt ttccgcgaat tcaccctgtt gcaaggtgcc aatcgagcag gcatgctcga
     3361 ccacttccag cccg
//
LOCUS       DATASH010000886         5525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5691667,
            whole genome shotgun sequence.
ACCESSION   DATASH010000886 DATASH010000000
VERSION     DATASH010000886.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5525
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5691667"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VHV99_23680"
     CDS             <1..273
                     /locus_tag="VHV99_23680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006401481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX3638419.1"
                     /translation="VALAAFAGVLAAPVIQVSPLMGQPMIITVFAVVVIGGMGSIMGS
                     ILTGLMLGVIEGLTRVFYPEASATVVFVIMAIVLLVRPAGLFGKEK"
     gene            273..1244
                     /locus_tag="VHV99_23685"
     CDS             273..1244
                     /locus_tag="VHV99_23685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018432441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX3638420.1"
                     /translation="MQRKVLYGLLLIGLVAVPVLGIYPLFVMKVLCFALFAAAFNLLI
                     GYTGLLSFGHAMFLASAGYVTGYAIQTLGFSPELGVIAGTAVATLLGLVVGFFAIRRQ
                     GIYFAMVTLALAQMVYFVFLQAPFTHGEDGLQGVPRGKLFGLLDLSSDVALYFVVLAV
                     MVLAFLLIVRIVHSPFGQVLVAIKENEPRAVSLGYDTDRFKLLAFILSAGLAGLAGSL
                     KVVVQGFETLGDAYWTMSGLVILMTLVGGMGTLFGPLLGATLIVALEDRLGDIGGALA
                     ATTGIDWFRSLGESVTIVTGVIFIACVLAFRRGIVGEIVARVRPLRA"
     gene            1778..2944
                     /locus_tag="VHV99_23690"
     CDS             1778..2944
                     /locus_tag="VHV99_23690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3638421.1"
                     /translation="MKSALRWMSAALVATGVSAAWSGHAFADVKIGVTVSATGPAASL
                     GIPEKNTIALLPKEVAGQKIDYIVLDDTTDSTEAVKNARKLTSEDHVDALIGSTVVPN
                     SLAMIDVAAESTTPMISMAAAASIVEPMDAKRAWVFKTPQNDILMATAIAQHMANHGV
                     KTVGFIGFADAYGESWFKEFGKAAELARIKIVANERFARNDASVTGQVLKIMSQNPDA
                     VLIAGAGTPAALPQKTLKERGYKGKYYQTHGVANNDFLRVCGKDCEGTYLPAGPLLVA
                     DQLPDSNPVKKPSLAYKHAYEAAYGAGSISTFGGHAWDAGLLLQHAIPVALKKGQPGT
                     PAFREALRAALEDTKDMPASHGIFNMSANDHAGLDQRARVMVEIVGGKWKLSGD"
     gene            3357..4517
                     /locus_tag="VHV99_23695"
     CDS             3357..4517
                     /locus_tag="VHV99_23695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015004091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3638422.1"
                     /translation="MKKTKQWVRTGIAMALMCGAGAAFAQVKIGVTLSTTGPAASLGI
                     PEKNTIALLPKEIGGKTVQYIILDDASDTGKAVQNTRKLIDEDHVDAIIGSTVTPNSL
                     AMLDPAAEGKTPVISLAASAAIISPMDAKRAWAFKPPQNDSLMADAIADYMEHHGVKT
                     VGFIGFADAYGDGWNNVFTAAAAAHHLTIVSNERFNRTDASVTGQVLKTMGANPDAVL
                     IAGAGTPSALPAKTLKERGYKGKVYQTHGVANNDFLRVCGKDCEGEFLPAGPILVADQ
                     LPDSNPVKKSSLAYKAAYEKAYGTGSVATFGGHAWDAGQMLEAAIPVALKTAQPGTEA
                     FRVALRGALENIKELPVSHGIMNTTTTNHNGLDKRARVIVQIVDGKWKLQND"
     gene            4726..>5525
                     /locus_tag="VHV99_23700"
     CDS             4726..>5525
                     /locus_tag="VHV99_23700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX3638423.1"
                     /translation="MDLSIAAILAQDGITTGAIYALLALALVLVFSVTRVIFIPQGEF
                     VSYGALTLAALQTQKFPATCWLLMAMGAACFVVEVAGLVRHPERRRHAARTLVMLVGK
                     YLLFPIAVYALTQGVFLHQLPMIAQIALTLLIVIPMGPFVYRLAYEPIAEATTLLLLI
                     VAVAVHFAMVGLGLVMFGAEGSRTTAFSDATFNLGSLSISGQSLWVVGTAAVLIGALY
                     LYFDRSISGKALRATSVNRLGARLVGIGTTQAGRLAFTLAAGLGALCG"
BASE COUNT         1036 a         1568 c         1793 g         1128 t
ORIGIN      
        1 gtcgcactgg cggcgtttgc cggcgtgctg gccgcgccgg tcattcaggt ctcgccgcta
       61 atgggtcagc cgatgatcat caccgtgttc gcggtggtcg tgatcggcgg gatgggttcg
      121 atcatgggct cgattctgac cggcctgatg ctcggcgtga tcgaagggct gacgcgcgtg
      181 ttttatccgg aagcgtcggc gaccgtcgtc ttcgtgatca tggcgattgt gttgctggtg
      241 cggccggcgg gtctcttcgg caaggaaaaa tgatgcagag aaaagtactc tacggtctgc
      301 tgctgatcgg gctcgttgcg gtgcccgtgc tcggcatcta tccgctcttc gtgatgaagg
      361 tgctttgctt cgcgctgttc gcggcggcgt tcaatctgct gatcgggtat accggcttgc
      421 tgtcgttcgg tcacgcgatg ttcctcgcgt cggccggcta cgtcaccgga tatgcgattc
      481 agacgctcgg cttttcgccg gaactcggcg tgattgccgg caccgcggtg gcgaccttgc
      541 tggggctggt cgtcggattc ttcgcgattc gccggcaggg catctacttc gcgatggtga
      601 cgctggcgct cgcgcaaatg gtctacttcg tgttcctgca ggcgccgttc acgcacggtg
      661 aggacggact gcaaggggtg ccacgcggca agttgtttgg tctgctggat ctgtcgtcgg
      721 atgtggcgct gtactttgtc gtgctggccg taatggtgct ggcgttcctg ctgatcgtgc
      781 ggatcgtgca ttcgccgttc gggcaggtgc tggtggcgat caaggaaaac gagccgcgtg
      841 cggtgtcgct cggctacgac acggatcgtt tcaagctgct cgcattcatt ctgtcggcgg
      901 gcctggccgg cctcgcgggc tcgctcaagg tggtggtgca gggcttcgag acgctcggcg
      961 acgcgtactg gaccatgtcc ggtctggtga tcctgatgac gctcgtgggc ggcatgggca
     1021 cgctgttcgg accattgctc ggcgcgacgt tgatcgtcgc gctggaagac cggctcggcg
     1081 atatcggcgg tgcgcttgcg gcgacgaccg gcatcgactg gttccgctcg ctgggcgagt
     1141 cggtgacgat cgtgacgggc gtgatcttca ttgcttgcgt gctggcgttc cggcgcggca
     1201 ttgtcggcga gatcgtcgcg cgggtgcggc cgctgcgggc ttgatgcgcg gcgacgtgta
     1261 atgacgcgca caattgattc ggcatccgat gtaattttca acattttgaa gactgctgct
     1321 ctagtgaatg gtggtatcgg agacatagtc aaagccgctg gcgacagcgc cagacgtgcc
     1381 ccatttcggt gccgcactgc accactaggg taattgctag ttgtagtcaa aggggtgctc
     1441 gtttaatctt catgcagttc tgatgcacca cgaattttgc aggtggagaa cgaggagaca
     1501 atgaggcact cagtgcccag acgaaagcgc tgttggattg atatccaaca gcgcttttta
     1561 tttgcacctt ttgtaaagta ccattcaaat ctcaagctaa ttcggtgccc cttttttatt
     1621 caccgaacca ctccccattt cacctgccgt gactccgcaa aatgcttgca gcgcggggct
     1681 ttcgtccgct aaactcccca ttcaccgacc gcctggtcgg cgattcaaat ccgcagattt
     1741 gaattcaatt gaataacggg gtagatagag gagcgggatg aagtcggcat tgcgttggat
     1801 gagcgcggca ctggtcgcca ccggggtatc cgcagcatgg agtggtcatg cattcgcgga
     1861 cgtgaaaatc ggcgtcacgg tgtcggcaac gggaccggcc gcgtcgctcg gtattccgga
     1921 aaagaacacg atcgcgctgc tgccgaaaga agtagcgggg cagaaaatcg attacatcgt
     1981 gctcgatgac accaccgatt ccaccgaggc cgtcaagaac gcacgcaagc tcaccagcga
     2041 agatcatgtg gatgcattga tcggctcgac cgtcgtgccg aactcgctgg ccatgataga
     2101 cgtcgcggcg gaaagcacca cgccgatgat ctccatggcc gccgcggcga gcatcgtcga
     2161 acccatggac gcgaaacgcg catgggtctt caagacgccg caaaacgaca ttctgatggc
     2221 cactgcgatc gcgcagcaca tggcgaatca cggcgtgaaa acggtcggct tcatcggctt
     2281 tgccgacgcc tacggcgaga gctggttcaa ggagttcggc aaggcagccg agctcgccag
     2341 gatcaagatc gtcgcgaacg aacgcttcgc gcgcaacgac gcttcggtga ccggccaggt
     2401 gctgaagatc atgtcgcaga atccggacgc ggtactgatc gccggcgcgg gcactccggc
     2461 cgcgctgcca caaaaaacgc tcaaggagcg cggctataag ggcaagtact atcagacgca
     2521 tggcgtcgcc aataatgact tcctgcgcgt gtgcggcaag gattgcgaag gcacctatct
     2581 gccggccggt ccgctgctgg ttgctgatca actgcccgat tcgaacccgg tgaagaagcc
     2641 ttcgctcgcc tacaagcatg cgtatgaagc cgcatacggc gcgggctcga tatcgacatt
     2701 cggcggtcac gcatgggatg caggcttgct gttgcagcac gcaattccgg tggcgctgaa
     2761 gaaaggtcaa ccgggcacgc ctgcgttccg cgaagcactg cgcgcggcac tcgaagacac
     2821 gaaagacatg cctgcttccc acggcatctt caacatgagc gcgaacgacc acgctggcct
     2881 cgaccagcgg gcgcgtgtga tggtggagat cgtcggcggt aagtggaagc tgtccggcga
     2941 ctgagcgaag gcgaagcgat acggaatctc atcaaaaaag acgcgagcag ttgacgcgtc
     3001 ttttttattg cccgtatcgg gtgaattgcg gtgcgtaaag tgggttggat aatcgaagaa
     3061 caagcgctcg aagcgcggtt gaagcggccg gtttgcggac caacccgcag ctttcggatg
     3121 agcgtctatt gaccgttgat gatgcaaacg caatacatac taatatgtta gtgttttgtg
     3181 cataagaaat cgcagtagtg gttcgcagca agggagtggc accagaagaa gggcgaaggc
     3241 attgatgctg agcagggaaa accatgcttg gcacgcgtga accctataac tagattcaga
     3301 cacccggccc gtgagccgga ttacagatac gcgctttaaa gcgttggaga cttgcaatga
     3361 aaaagacaaa gcaatgggta cgcaccggta tcgcgatggc gttgatgtgc ggcgcgggcg
     3421 cagcctttgc gcaggtgaaa atcggcgtta cgttgtccac cacggggccg gctgcatcgc
     3481 tcgggattcc tgaaaagaac accatcgcgc tgctgccgaa agagatcggc ggcaagacgg
     3541 tgcaatacat catcctcgac gacgcatcgg acaccggcaa ggccgtgcag aacacgcgca
     3601 agctgatcga cgaagaccac gtcgacgcca tcatcggttc gaccgtcacg ccgaactcgc
     3661 tggcgatgct cgatcccgcg gcggaaggca agacgccggt gatctcgctg gcggcatcgg
     3721 ccgcgatcat ttcgccgatg gacgccaagc gcgcatgggc cttcaaaccg ccgcaaaacg
     3781 acagcctgat ggctgacgca atcgccgact acatggagca tcacggcgtg aagacggtcg
     3841 gcttcatcgg cttcgcggat gcgtatggcg acggctggaa caacgtcttt accgctgcgg
     3901 ccgcggcaca tcatctgacg atcgtctcca acgaacgctt taaccgcacc gatgcatcgg
     3961 tgaccggcca ggtgctgaag acgatgggcg cgaaccccga tgcggtgctg atcgcgggcg
     4021 cgggtacgcc gtccgcgttg ccggccaaga cgctcaagga gcgcggctac aagggcaagg
     4081 tctatcagac gcacggcgtc gccaacaacg acttcctgcg cgtgtgcggc aaggactgtg
     4141 aaggcgagtt cctgccggcc ggtccgatcc tcgtggcgga tcagttgccg gactcgaacc
     4201 cggtgaagaa gtcgtcgctc gcgtacaagg cagcgtatga gaaagcgtac ggcacgggct
     4261 cggtggcgac cttcggcggt cacgcatggg acgcgggcca gatgctggaa gccgcgattc
     4321 cggtcgcgct gaagaccgct caaccgggca ccgaagcgtt ccgggtggca ctgcggggcg
     4381 cacttgagaa catcaaggaa ctgccggtct cgcacggcat catgaacacc acgacgacca
     4441 accacaatgg cctcgacaag cgcgctcgcg tgatcgtgca gatcgtcgac ggcaagtgga
     4501 agttgcagaa cgactaataa caaaagcggt acgcgcctct cgcgcatttg cggtgcatga
     4561 ctgacgccgc attgccattc tgttcgatga caatggcaac gaccgccgcg ccgtgtcccg
     4621 gcgcggcggt cgccttttct ggcactgcag gtccgtggcc ggccgcgggt tctgccgcca
     4681 cgactgtttt ccggtttcga tttcgacgct tcaggacgag gaagtatgga tctatcaatt
     4741 gcggcgatcc tcgcgcagga cggcatcacc actggcgcga tttacgcgct gctcgcgctc
     4801 gcgctggtgc tggtgttctc cgtgacgcgg gtgattttca tcccgcaggg ggaatttgtt
     4861 tcgtacggcg cgttgacgct cgccgcattg cagacgcaga aatttccggc gacctgctgg
     4921 ttgctgatgg caatgggcgc ggcgtgcttc gtggtcgagg tggcggggtt ggtgcgccat
     4981 cctgagcggc ggcggcatgc ggctcgcacg ctggtgatgc tggtgggcaa atacctgctg
     5041 tttccgatcg cggtttacgc gctcacccaa ggcgttttcc tacatcagct accgatgatc
     5101 gcgcagatcg ctctgacgct gctgatcgtg attccgatgg ggcccttcgt ctatcggctc
     5161 gcctatgagc cgatcgcgga ggcgaccacg ctgctgctgc tgatcgtggc ggtggcggtg
     5221 cactttgcga tggtcggcct cgggctcgtg atgttcggtg cggaaggctc gcgcaccacg
     5281 gcgttctcgg atgcgacttt caatctcggc agcttgtcga tttccgggca gagtttgtgg
     5341 gtggtcggta cggcggcggt gctgatcggt gcgctgtatc tgtacttcga ccgttcgatc
     5401 tccggcaagg ctttgcgcgc gacctcggtg aaccggctcg gcgcgcggct cgtcggcatc
     5461 ggcacgacgc aggctgggcg cctcgcgttc acgctcgccg caggcctcgg tgcgctgtgc
     5521 ggcat
//
LOCUS       DATASH010000887         3909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5292582,
            whole genome shotgun sequence.
ACCESSION   DATASH010000887 DATASH010000000
VERSION     DATASH010000887.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5292582"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="VHV99_23705"
     CDS             complement(<1..454)
                     /locus_tag="VHV99_23705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016347958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HEX3638424.1"
                     /translation="MESGYGVGIQSEREAALQQSQQKQDAPDTPGMYLALIDRMQSQG
                     LFYASLAHIDAYEKQYGSTPDTILLRADALRQTGQAQGSADAYTKLLNTPLAARGYRG
                     LGLLAGAAGEFAKASEEFGAATRLQPTDALTLSDLGYARVRLGDVAGAG"
     gene            complement(579..1589)
                     /locus_tag="VHV99_23710"
     CDS             complement(579..1589)
                     /locus_tag="VHV99_23710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006764470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX3638425.1"
                     /translation="MTAQTVFVLALLFAALGCALLGALAFTGLARMQRGARTLDHALD
                     QRAASSAARAIAAGHERRPTTDAKYAGEAGRGRANGGCLSRLADALSRVAVSWLDTPF
                     GAIVVAPEDRRLLDRCGFPDAKTRARFFAARVGCAALLPLLALGWFNGEPHLAMWMIA
                     AFVLGFMLPKWFVRRRATARQRSVTSELPMFVDLLRLLQGVGLSLDQSLQVMINDFAT
                     VLPVLSQELALAHRQFTTGRTREQSLSRLATSYESDDLNAVMRLVVQVDKHGGAVQEP
                     LRQFGDRLRETRRAALRERIGALTVKMTGVMVLTLLPALLIMTAGPGFIAVFHSLSTM
                     HR"
     gene            complement(1592..2605)
                     /locus_tag="VHV99_23715"
     CDS             complement(1592..2605)
                     /locus_tag="VHV99_23715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX3638426.1"
                     /translation="MSSGIGLVLVAALLCAAGGLLLWQRAHDNCERALTDRFVDRRVA
                     SVQGAMPGATSGTAPAQNRQRTISAAPAALSPNAAHGGREHLRHLQARLAHGADNLMN
                     RAGIASPRGLLWTACVVILPGACLVGLRTNSIVGLGFIVFGALCVFGFLSWRAQKRYR
                     HISRQLPIFLDGIVRLITIGNSVPAAFQAALATTDAPLRDCLDRVSRMLRSGIEIDVA
                     LRHVASVYRVRELELVGSVLRISVKYGGRSDVMLDRMAALMRDLEQAERELVALSSET
                     RLSAWVLGLLPALLGGFVMLSNPSYFLEMWSDPFGQRLVYVALGLQALGAFMLYRLVR
                     LKD"
     gene            complement(2598..>3909)
                     /locus_tag="VHV99_23720"
     CDS             complement(2598..>3909)
                     /locus_tag="VHV99_23720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006400297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HEX3638427.1"
                     /translation="AAHEHLLTRIEELGAEFGRWSRQAIQQFVDLEIDGFVRMRRIAL
                     NESEVRLVAQALTKELAGFGPIEDLLADPAVEDILINGFDDVYVSRHGMLVKIPVRFA
                     DNAHLLRIVRRILAPIGRRLDESNPMVDARLPDGGRVNVVIEPLSLDGPVVSIRKFRR
                     DPLRPDDLLSNGTFDHEICGLLDAAVAARCNVLVSGGTSSGKTSLLNALAFFIPKHER
                     VVTIEDTAELSLNHPHVVRLESRPGGFDGAGVVSIRDLLRNTLRMRPDRIIVGEVRGG
                     EVLEMLQAMNTGHDGSMGTVHASSARECLYRLEMLAGFAGFQGTESSLRRQIANAIDF
                     IVQIGRLSNGRRRILSVTEVTGLSDNIVATQELYRYEPIMEGGGELDNWVSLGIHPSS
                     PKLAKYRQSLGNDRSTDSFNDGFGRRDGGGGGGGFGRSGGGFNV"
BASE COUNT          797 a         1328 c         1157 g          627 t
ORIGIN      
        1 cgcctgcacc cgccacatcg ccgagacgca cgcgcgcgta acccagatcc gatagtgtga
       61 gcgcgtcggt tggctgcagg cgcgtggcag caccaaactc ttcggatgcc ttagcaaatt
      121 cccctgctgc gccagccagc aaaccgagac cgcgataccc ccgcgccgcc agcggcgtgt
      181 tcagcaattt cgtgtatgca tcggcgctgc cctgggcctg gccggtctga cgcaaggcgt
      241 cggcgcgcag cagaatcgtg tcgggcgtcg agccatactg tttctcgtac gcgtcaatgt
      301 gcgcaagcga cgcatagaac aaaccctgcg actgcatccg gtcgatcagc gcgagataca
      361 tgccgggtgt atcgggcgcg tcctgcttct gctgcgactg ctgtagcgcg gcttcgcgct
      421 ccgactgtat gccgacgcca taaccgcttt ccatctttga ggcacaacca cccagccccg
      481 ctaaaacggt ataggcaagc gtcaggacca caacgggcag gcggttctcg attgcgattg
      541 atttcatggg gtcactctct tcaacgtctc ttcaatgtct atctatgcat cgtggacagc
      601 gaatggaaca cggcgataaa ccccggaccg gctgtcatga tcaacagcgc gggcagcaag
      661 gtcagcacca tcacgccagt catcttcacg gtcaatgcgc cgatccgttc acgcaacgcc
      721 gcgcggcgcg tctcgcgcaa ccggtcgccg aactggcgca gcggttcctg taccgcccca
      781 ccgtgcttgt cgacctggac caccagccgc atcacggcat tcaggtcgtc gctttcatag
      841 cttgttgcga gccggctcag cgactgctca cgcgtacgtc cggtggtgaa ctgccggtgg
      901 gccagcgcga gttcctgcga gagcacgggc agcacggtcg cgaagtcgtt gatcatcact
      961 tgcaggctct gatcgagcga caggccgacg ccttgcagca aacgcagcaa gtcgacgaac
     1021 atcggtaact cactggttac cgagcgttga cgcgccgtcg cgcggcgccg cacgaaccat
     1081 ttcggcaaca tgaaacccag cacgaacgcg gcaatcatcc acatggcgag gtgcggttct
     1141 ccgttgaacc agcccagcgc aagcagtggc aacagcgcgg cgcaaccaac ccgcgccgcg
     1201 aagaaacgcg cgcgtgtttt ggcatcgggg aaaccacatc gatccagcaa acgccggtct
     1261 tcgggcgcca caacaatggc gccgaacggc gtatccagcc aactcaccgc cacgcggctg
     1321 agtgcatcgg ccaggcgcga caggcagccg ccattggccc gtccccgccc cgcctcaccc
     1381 gcgtacttcg cgtctgttgt tggccgccgt tcgtggcccg ctgcaatcgc gcgtgccgcc
     1441 gaactggccg cgcgttgatc gagcgcatgg tcgagcgtgc gcgcgccgcg ctgcatgcgc
     1501 gcgaggcccg tgaacgcgag tgcgcccaac aaagcgcaac cgagtgcggc gaagagcaag
     1561 gcaagaacga acacggtttg ggcggtcatg atcaatcctt caggcgcact agccggtaca
     1621 acatgaaagc accgagcgct tgcagtccca gcgccacata cacgaggcgc tgaccaaaag
     1681 gatcggacca catctccagg aaataggacg gattggagag catcacaaaa ccgccgagca
     1741 aagccggcaa gagcccaagc acccaggcag acaaacgcgt ctcggaggac agcgcaacca
     1801 gttcacgctc ggcctgttcg aggtcgcgca tcagcgctgc catgcgatcc agcatcacgt
     1861 cggaacggcc accgtacttc accgagatgc gcagcaccga accgaccagc tccagctcgc
     1921 gcacgcgata caccgaagcc acgtgtctca gcgccacatc tatttcgatg cccgagcgca
     1981 acatccgcga gacccggtcg aggcagtcgc gtaacggcgc gtcggtggtc gccagggcgg
     2041 cctggaacgc ggccggcacg ctattgccga tcgtgatgag ccgtacgatg ccatcgagga
     2101 atatgggcaa ctgacgcgag atgtgccgat accgcttctg cgcgcgccac gagagaaaac
     2161 caaacacgca aagcgcgccg aagacgataa agcccaatcc cacgattgaa tttgtccgca
     2221 gaccaaccag acatgcacct ggcaagataa ccacgcaagc ggtccataag agcccgcgcg
     2281 gactcgcgat acccgcgcgg ttcatcaggt tgtcggcgcc gtgagcaaga cgggcctgca
     2341 gatgacgcag atgttcgcgg ccaccgtgcg cagcattcgg tgacagcgcg gcgggagccg
     2401 cagatatagt gcgctggcgg ttctgggcag gcgctgtgcc cgatgttgcg cccggcatcg
     2461 cgccctgtac tgaagctacg cgccggtcga cgaaacgatc ggtcagcgcg cgttcgcagt
     2521 tgtcatgcgc gcgctgccac agcagcagcc caccggctgc gcacagcagc gctgcgacca
     2581 gcacgagacc gataccgcta gacattgaag ccccctccgg agcggccgaa accaccgcct
     2641 ccgccaccgc cgtcacgccg tccgaagccg tcattgaagc tgtctgtgct cctgtcgtta
     2701 ccgagggact gacgatactt ggcgagcttc ggcgagctgg gatgaatgcc gagcgagacc
     2761 cagttgtcca gctccccgcc accttccata atcggctcgt accggtacaa ctcctgggtc
     2821 gcgacaatgt tgtccgagag ccctgtcact tccgtcaccg acaaaattcg ccgccgtcca
     2881 ttggacaggc gcccgatctg cacgatgaag tcgatggcat tggcaatctg ccgccgcagg
     2941 ctcgactccg tgccctgaaa gccggcaaag cccgccagca tctcgagccg gtaaagacac
     3001 tctcgcgccg agcttgcgtg gacggtgccc atcgaaccgt cgtgcccggt gttcatagcc
     3061 tgcagcattt ccagcacttc gccgccgcgt acctcgccca cgatgatcct gtccggacgc
     3121 atccgcagcg tattgcgcaa gaggtcgcga atggacacca cgcccgcacc gtcgaacccg
     3181 cctggacggc tttccagccg caccacgtgc ggatgattaa gcgagagttc ggcggtgtcc
     3241 tctattgtca ccacgcgctc gtgcttcgga atgaaaaacg cgagcgcatt gagcaacgag
     3301 gtcttgcccg aactcgtccc gcccgacacc agcacgttgc agcgcgcagc gaccgctgcg
     3361 tcgagcaacc cgcagatctc atgatcgaag gtgccgttcg agagcaggtc gtcggggcgc
     3421 agcggatcac gacgaaactt gcgtatggat accaccggcc cgtcgagcga cagcggctcg
     3481 atcaccacgt tcacccggcc gccgtcgggc aggcgggcat ccaccatggg attggattcg
     3541 tccaggcgac gcccgatcgg ggcgaggatg cgccgcacga tccgcagcaa gtgcgcgttg
     3601 tctgcgaagc gcaccgggat cttcacaagc atgccgtgcc gcgacacata gacgtcgtcg
     3661 aagccgttga tcaggatgtc ctccacggcg ggatcggcga ggagatcttc gatcggcccg
     3721 aagcccgcca actccttcgt caacgcctgc gcgaccagcc gcacttcgct ctcgttgagc
     3781 gcaatacgcc gcatccggac gaagccgtcg atctccaggt caacgaactg ctggatcgcc
     3841 tggcgtgacc agcgtccgaa ctcggcgccc agctcttcaa tccgcgtaag cagatgttcg
     3901 tgcgccgcg
//
LOCUS       DATASH010000888        17077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1536071,
            whole genome shotgun sequence.
ACCESSION   DATASH010000888 DATASH010000000
VERSION     DATASH010000888.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 17077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17077
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1536071"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..984
                     /locus_tag="VHV99_23725"
     CDS             94..984
                     /locus_tag="VHV99_23725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010093171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX3638428.1"
                     /translation="MRIPPLKAIVAFESVARTKSVNRAADELGLTPSAVSHQIANLES
                     MVGRPLFTRLGRGLVLTPTGQQYLADVTGSLADLSRATERASSQSGVEILRIHSSPSF
                     GLMWLLPRLASFHEANGDIQLNLACSYEDVSFTSGYYDLDVRHGYAHWADGEIKTLRN
                     EFIAPLASEEYLRKFPVRTPEDLLERRLIFSETPLVQWKQWFGKFGVAVSRKAYDFAF
                     DRSYMSLEAAALGHGIALESTMLASVHLKRGSLVPVFDHTYAVEVGAHHLVYPSQNAD
                     LPRVAKFLTWVEQEISRHAM"
     gene            1174..1923
                     /locus_tag="VHV99_23730"
     CDS             1174..1923
                     /locus_tag="VHV99_23730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3638429.1"
                     /translation="MLLDSKVVIVTGAASPRGIGKATAKALAAQGARVVILDLKKEDA
                     ESAARDLGAEHLGLPCDVTDKKACIAAANAVIEKYGRVDGLINNAGITQPLKTMDIEA
                     ANFDAVVDVNLRGTLYMSQAIIPQMRKQKSGSIVCMSSVSAQRGGGIFGGPHYSAAKA
                     GVLGLARAMARELGPDNIRVNSITPGLIQTDITGDKLTTEMRADVIKGIPLGRLGDAA
                     DVANACLFLSSELSAYLTGITLDVNGGMLIH"
     gene            2001..3329
                     /locus_tag="VHV99_23735"
     CDS             2001..3329
                     /locus_tag="VHV99_23735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012405100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3638430.1"
                     /translation="MKTKATAPSMTGDVTSLADSASFESKTYAKVGRRLIPFLMLCYL
                     GAYLDRVNVGFAKLQMLNDLRFSETVYGVGAGVFFLGYFLFEVPSNLLLHRVGARNWL
                     ARIMLTWAVISACFVFVKSPTSFYVLRFMLGVAEAGFAPGVILYLTYWFPSARRAKAL
                     SLFFMAIPLAGIVGGPLSGYILHAFQGVHGLAGWKWLFILEALPSLCLGVAILFYLDN
                     GIAAAKWLTDAEKALLVRNVANDNAHKTAHVSIKSFIGDRRLWLMASIYFCVVLGQYG
                     LTFWLPTIIRKSGVADPLWVGIFTAIPYLCAIIALPLVGASADRHRERRLHLAIPMLV
                     SAAGFATLPMLGGVGASLVCVSLAAAGILASSSQFWSLPTALLGGMSAAAGIAAVNCF
                     ANLAGFFSPAIVGWLNDLTGKSTAGLIFISVAIVIGAGLVFLVPAKTVNR"
     gene            3375..4217
                     /locus_tag="VHV99_23740"
     CDS             3375..4217
                     /locus_tag="VHV99_23740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006397970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX3638431.1"
                     /translation="MNSPSSDTVALAEHAYRIRRNALLMGEVQGQGYIGQALDIADVL
                     AVAYFGAMRYQPDNPEWEGRDRFLLSNGHYAIALYAALFEAGFLPPEELETYGSDDSR
                     LPMSGMASYTPGMEMSGGSLGHGLTIAVGRCLGLKRKGSDAFVYTLFSDGELDEGAIW
                     EGLMSAAHWKLDNLIAMVDVNNQQADGPSSKIMAFEPLVEKLEAFGWFTQRVDGNDID
                     AVKAAFDAARNHPKEQPRIIVCDTSMGCGVPFLEEREKNHFIRVDAHEWQLALAALEA
                     GRQA"
     gene            4214..5215
                     /locus_tag="VHV99_23745"
     CDS             4214..5215
                     /locus_tag="VHV99_23745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEX3638432.1"
                     /translation="MSNTTTQKPRLKTSAMIASIAGEGQVTRSAPFGHALAQLAREKK
                     NVIGMTADLGKYTDLHIFGKEFPERYYQMGMAEQLLMGAAAGMAHEGAQPFVTTYAVF
                     ATRRAYDFMHQAIAEDNLDVKIICALPGLTTCYGPSHQAAEDLALMRAMPNMTVIDPC
                     DALDTEQMVPAIAAHNGPVYARLLRGNVPAVLDEYDYQFELGKAKLLRDGTEVLIISS
                     GIMTMRSLEVAKLLEADNVGVGVLHVPTIKPLDTETILREARRTGRMVVVAENHSVIG
                     GLGEAVATTLLTAGVTPMYRQIALPDEFLGAGALPTLHDRYGISTNVMADTIKGWLG"
     gene            complement(5730..5981)
                     /locus_tag="VHV99_23750"
     CDS             complement(5730..5981)
                     /locus_tag="VHV99_23750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018432656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-binding protein"
                     /protein_id="HEX3638433.1"
                     /translation="MSHVVVERSFPTPQSDAELSVVEDRLAPCSELHGIVWKRSVVSI
                     DRRHMICEYEAPDAETVRKVQREAGAAFDFIWSGDVIES"
     gene            complement(6023..9190)
                     /locus_tag="VHV99_23755"
     CDS             complement(6023..9190)
                     /locus_tag="VHV99_23755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3638434.1"
                     /translation="MRNADAPDAPVGPLTIRLLGELEVRRDAASVPLPPSRRTRALLG
                     YLVMSDGAIPRSALCDLLWDGPDDPRAALRWSLSKLRPLVDDPSQERIVANRTHVAFD
                     AAGAFVDSNRIQALLASGTERLPVAALAEAAQLLRGDFLGGLELPGCYRFHNWCVGQR
                     ERFGRIRGTVLRALIAALSCDLPLALRYGRELVETDPLDESAHATLISLLEATGRRSD
                     GDCHYRYARELLHREMGARACAMLDDAVRRVRHTTSLRHTSEYDHCALGDESRPSKHD
                     AGAAGQNERTPTRDQQPMAPLPLVGRGAERAKLDELLATSTPHGLTLLTGEPGIGKTR
                     LLDYFSQCASNASYRVLRSRCYEAEAVRPYGIWIDALRDLGIDALQPVDEHLAPFIRP
                     AGPGTPEMQRSERRRARFFEAVVGLLGRLCAEDRLAFVLDDLQWLDESSAALLHFVLR
                     RLDHELPVVFVAAARPAETEDNRSARILLHSLARDGRLIHVPLHPLSANEVGTLIEAS
                     GSDIRVEQALTDSGGNPLYLLELTRANRMDREPWTCTVDTLIADRLAPLDGPTRDLLS
                     FAASIGRAFAPEQLAQLLDRPLPELLSCVAELQRRGILAETTSTEFDFAHDLVRQAVY
                     RTLSQPHRRAIHRQIARQLLAESKHDPRLHGELAHHATLGGDSRMTARACVEASNHCL
                     RVFAATEARTLADRGLAHARSLPHGSERVQIEIQLLTARLVAVASSGEACAASMEQEF
                     EQAIHDAETLSLHADVVQGLHSLSWLTQQANDIERTRQVTIRAEMAARKADAATRCKQ
                     LANTGRCLLELERDLPRARGVLQEAGVLAEHLDLRVLELMWGEALLARADSELDIGCV
                     KLDEAVAQARAIGDHWREYQCLVWLATMTFERGAYSDVTRLAGEIVAAAQKMGDSGAP
                     FAEVISQISALRLPAVAPGMFAFEGLDALRRADDKRHLCYALNEVARVCLDRRCSADA
                     SALGHQALQAANALDSPTERIVATALLIEAAFAADKTEASDRLLSSLRELLGDKPLAP
                     RSRAVLQRLYDRYPGITTILQTQPH"
     gene            complement(9457..9801)
                     /locus_tag="VHV99_23760"
     CDS             complement(9457..9801)
                     /locus_tag="VHV99_23760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638435.1"
                     /translation="MLRSVVLSLCNYFKAQPLHLLGFHQKRYEQRMKRLPPHVRITAR
                     MDRCGDARSEKRPGRRKSGCAANRERLLFGHPVLLARRIVLEMAVRRLRRQPLADCSR
                     IGTRLLRKTLRR"
     gene            complement(10133..10825)
                     /locus_tag="VHV99_23765"
     CDS             complement(10133..10825)
                     /locus_tag="VHV99_23765"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3638436.1"
                     /translation="MKPSIVFSHGIWADGSCFQKLIPTLRAEGHEVMAAQYGLDTVKG
                     DVDATIRTFGRVSGPIVLVGHSYGGTVITHAGMDPRVAALVYIAALAPDETETSQGQQ
                     EKFATTDIFENIDVADGRVWLKPGSGVPCFAGDLSADEQEIAYATHFAPNADLFNQKL
                     DGIAWQSKPSWYIVATEDRTVNPELQRFVSKRMGATTVELKTSHVPMLSQPHAVLDVI
                     RKACAAVAAKQG"
     gene            complement(11236..14382)
                     /locus_tag="VHV99_23770"
     CDS             complement(11236..14382)
                     /locus_tag="VHV99_23770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012211067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HEX3638437.1"
                     /translation="MLPASFGLPGYAELQCASNFTFLQGASHAEELATRAAQLGYEAI
                     AITDECSLAGIVRAHVAAKETGIKLLVGAHFRVTNADGSPALAFTAIAQNRDGYGNLS
                     ELITLARTRTAKGTYVVYPHDLDHPDSPYTHLRALPDCLIILTPEFPAKDSRLDAQAQ
                     WAAQTFGTDRCWMGFTLHARALDDLHRNTVYDVAERHGLNVVATGQVLMHVRSRKPLQ
                     DTLTAIRLNRPVTECGFELTPNAEQHLRSRLRLANVYPADALAQTLNIASRCTFMLDE
                     LRYEYPDELVPPGTTPTAYLRRETYLGAQRRYPRGIPHSVQKQIEHELSLIADLKYEP
                     YFLTVYDIVRFARNEGILCQGRGSAANSAVCFCLGVTEVDPDRSSMLFERFISKERGE
                     PPDIDVDFEHQRREEVIQYIYRKYTRTRAALAAAVTTYRPRSAIRDSGKALGIDPMIV
                     DRVAKEHHWFDSSADLIRRFVESGLDPDTPMIQQWATLAAQLLGFPRHLSLHTGGFVI
                     AGGKLSRLVPVENASMQDRTVIQWDKDDLEALGLLKIDVLALGMLSAIRRTLELVSQQ
                     RGEPFEMQDIPPEDEATYDMICAADTVGVFQIESRAQMSMLPRLKPRKFYDLVIEVAI
                     VRPGPVQGGMVHPYLRRRQGLEPVSYPSPDVEQALKRTLGVPIFQEQVMQVAMLAAGF
                     SAGEADQLRRAMAAWKRKGGLQHYHDRIVEGMLQRGYDREFAESIFAQIQGFGEYGFP
                     ESHAASFALLVYASCWLKRHEPAAFLCAMLNSQPMGFYSPSQLVQDAKRHGVGVLPVD
                     VTISGWDSTMEGEGSMAPVRLGMSVIRGMREETARRIEAARAVRPFRDVADLANRSQL
                     DRHDLQVLAAANALKPLAGDRRQALWAAVAAVPDKDLLRATSREEDAPALTAPTEGQD
                     LIADYRSTGLTLGRHPLALLRKKLLSMRLMPAEALMRYRDGQLARACGIVTVRQRPGT
                     ANGVMFMTLEDETGSVNVIIWQSVLERQRREALGASLLAVYGVWQRQGDVKHLVAQRL
                     VDVSDLLGELPTASRNFH"
     gene            complement(14386..15858)
                     /locus_tag="VHV99_23775"
     CDS             complement(14386..15858)
                     /locus_tag="VHV99_23775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012211068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HEX3638438.1"
                     /translation="MQLWIGVHLPSLCLEVFRPKWSLEPDDDAGLAVLEKERVVAADP
                     SARAAGVCIGMRRGGVLTLAPATVMQERNSTAEVNAVREIATGLLNLSPQVVIAEEST
                     VLVDVSASLRLFGGIRPVRRMARRVADAMGVTATISVAPTGQAAWLLARYCGGVALSH
                     ASLSRALAGLPVNLPTPARRHLDWLTGIGCETVGQLMRLPRAGLKKRCGTALLDVLDR
                     ANGVAPEVYEWFETPPTFQARVELPDRIEHAEACVFAARRLVVQLTGWLSQKQLAVTR
                     IALLLEHERGRDAIDPTTIEVSLAEPTWREDHLMRLLRERLGRVELAAAVIALQLVVQ
                     EVKEAEPASDSLFPEPGGTAADHARLLELLVARLGAENVLKPAPVADHRPEVAARWVP
                     VTDKTKYVAPATDTPRPAWLLESPIKLLMRGERPFYGSPLRTVSSGERVEAGWFRGEP
                     VTRDYFVAEADDYICYCIYRERIGAVDEDEPRWYLHGLFG"
     gene            complement(15788..16495)
                     /gene="imuA"
                     /locus_tag="VHV99_23780"
     CDS             complement(15788..16495)
                     /gene="imuA"
                     /locus_tag="VHV99_23780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007589704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion DNA synthesis-associated protein
                     ImuA"
                     /protein_id="HEX3638439.1"
                     /translation="MSAVMLPEAIHPDLWKGNQLARAASRVVPCGDEALAAELPGGGW
                     PRGALTDLLVQQTGSGELRLLRPAFERLGSKPVVLLQPPHNVQPTAFAWWGLDPSILL
                     TVKASKTADALWAAEQILRAGTCGALVFWQQHVRPEAVRRLHLAAQTSESLFFMVRPL
                     ATTRDASPAPLRFAIRAAKGGVDVQFLKRRGPARDEPLFVSLTPSPILNVKRHATVDR
                     RPSPVVVPRSVPAEVVA"
     gene            16890..>17077
                     /locus_tag="VHV99_23785"
     CDS             16890..>17077
                     /locus_tag="VHV99_23785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HEX3638440.1"
                     /translation="MDIVRRSHRTDISGFADGRIGPYIAAFKQYLSQRRYAAKTVATY
                     LLCITHFAQWADSGPCRRR"
BASE COUNT         3194 a         5356 c         5335 g         3192 t
ORIGIN      
        1 cctctcagat gcgccaaatt tctggaatcg tcagtagcga agcagaagaa caccctgaag
       61 caagacggtt cgcgacccct gtacccagga acaatgcgca tccctccact aaaagccatc
      121 gtcgcgttcg agagtgtcgc ccgcacaaag agcgtcaatc gggcggctga tgaattaggg
      181 ttaacgccat cggcggtcag ccatcaaatt gccaacctcg aatccatggt cggaagaccc
      241 ttgttcacga gactggggcg gggactcgtg ctgacgccaa cgggtcagca atatctggcc
      301 gatgtcaccg gatcgctcgc cgatctgagc cgcgctacgg agcgcgcatc aagccagtcg
      361 ggtgtggaga ttcttcgcat tcattcgagc cccagcttcg ggctgatgtg gcttctgcca
      421 cgcctcgcgt cattccatga agcgaacggc gacattcagc tgaatctcgc gtgctcatac
      481 gaggatgtgt cgttcacctc cggttattac gatctcgatg tacggcacgg ctacgcccat
      541 tgggccgatg gcgagatcaa aacgcttcgc aatgaattca tcgcccctct tgcatcggag
      601 gaatacctga gaaaatttcc ggtgcgcacc cccgaggacc tgctcgagcg ccgcctgatc
      661 ttctctgaga ctccgctggt gcagtggaaa caatggttcg gcaagttcgg cgtcgcggtg
      721 tcccgaaagg catacgattt cgcgttcgac cgctcgtaca tgtcgctcga ggcggcggct
      781 ttgggacacg gcattgctct cgaaagcacg atgctcgcat cggtccatct gaaaagaggt
      841 tcgctcgtac cagtcttcga tcacacgtat gcggtcgagg tcggagccca tcacctcgtt
      901 tacccttccc aaaacgcgga cttgccgcgc gtggccaaat ttttgacctg ggtcgagcaa
      961 gagatcagcc gtcatgccat gtagcgccag gccacgtcag gcgggatgtc tgcggcctga
     1021 cgtatgcgaa aagcgcagct ttccacgcat gtgactgatc ggcgcaacgt cacctgaaaa
     1081 tctttcagca atagctgagc ctgactgcat tgatcgcaac ggttgaagcg cagaaactgc
     1141 gtctaccaac taacacgatc gatggagaca aatatgttgc tcgacagcaa agtggtaatc
     1201 gtcacaggtg cagcctcgcc gcgtggcatc ggcaaggcaa ctgcaaaagc actggctgcg
     1261 cagggtgcgc gagtggtgat cctggacctt aaaaaggaag acgccgaaag cgctgcgcgc
     1321 gatctcggcg cagagcatct tggactcccc tgcgatgtga ctgacaagaa agcgtgtatt
     1381 gcagcggcga acgcagtcat cgagaagtac ggccgggttg acggcctgat caacaacgcg
     1441 ggcatcaccc agccgttgaa gacgatggat atcgaagcgg ccaacttcga tgcggtcgtc
     1501 gacgtgaatc tgcgcggcac gctctatatg tcgcaagcga tcatcccgca gatgagaaag
     1561 caaaaaagcg gcagcatcgt ctgcatgtca tccgtttccg cgcaacgcgg cggcggcatc
     1621 ttcggcggtc cgcactatag cgccgccaaa gcaggtgtgc tcggtctcgc tcgcgcgatg
     1681 gcgcgtgaac tcggcccgga caacatccgc gtcaactcga tcaccccggg tctcattcag
     1741 accgacatca ccggcgacaa gctcacgacg gagatgcgcg ccgacgtcat caagggcatt
     1801 ccgctcggca ggctcggcga cgccgccgac gtcgcgaacg cgtgtctgtt cctctcaagc
     1861 gagctgtcgg cttacctcac cggcatcacg ctcgacgtca acggcggcat gctgattcac
     1921 taagtacgac atccgccgcg attgcggcgg atgagtcaat gcacccgaga aaacccgggg
     1981 cgatctggca ggagacaatc atgaagacga aggccactgc gccgtcgatg acgggcgatg
     2041 tcacgtcgct cgccgattcg gcgagcttcg aatcaaagac gtatgcgaaa gttggacggc
     2101 gcctgatacc gttcctgatg ctgtgctatc tgggcgcgta tctcgatcgc gtcaacgtgg
     2161 gctttgcgaa actgcagatg ctcaatgacc ttcgattcag cgaaacggtg tatggcgtcg
     2221 gcgctggagt tttctttctc ggttatttcc tctttgaggt accgagtaat ctcctgctgc
     2281 atcgagtggg cgcgcggaac tggctcgctc gcatcatgct gacgtgggcg gtcatttccg
     2341 cgtgcttcgt cttcgtgaaa tctcccacca gcttctatgt tctccgcttc atgttgggcg
     2401 tggccgaggc ggggttcgcg ccaggcgtca tcctgtatct gacgtactgg tttccttcag
     2461 caaggcgcgc aaaggcgctc tcactcttct tcatggcgat tccgctggcc ggcattgtcg
     2521 gcgggccgct gtccggttac atcctgcatg cgttccaggg tgtgcatggc ctcgcgggct
     2581 ggaagtggct gttcatcctt gaagcgctgc cgtcgctctg ccttggcgtg gcgatcctgt
     2641 tttacctcga caacggcatt gcggccgcca aatggctcac cgatgcggag aaggcgctcc
     2701 ttgtgcgaaa cgttgcgaac gataacgcac ataagaccgc ccatgtctcg atcaaatctt
     2761 tcatcggcga tcggcgtctg tggttgatgg cttcgatcta cttctgcgtg gtgctcggcc
     2821 agtacggtct cacgttctgg cttccgacga tcatccgcaa gtcgggcgtc gctgatccgc
     2881 tgtgggtcgg tatcttcacg gccattcctt acctctgcgc catcatcgcg ttgccactgg
     2941 tgggcgcaag cgctgaccgc catcgcgaac ggcgcctgca tcttgcgatc ccaatgctcg
     3001 tatcagcggc aggctttgcg acgctgccga tgctaggcgg agtcggtgca tcgctggtgt
     3061 gtgtcagcct ggctgccgcc ggcattctcg cttcgtcgtc gcaattctgg tcactgccga
     3121 ccgcgctcct tggtggcatg tccgcagcgg cgggtatcgc cgcggtgaat tgcttcgcca
     3181 atctcgcggg gttcttctcg ccagccatcg tgggctggtt gaacgatctg accggcaagt
     3241 cgacggctgg gctcatcttt atctccgtcg ctatcgtcat cggtgccggg cttgttttcc
     3301 tcgtgccagc caaaaccgtt aatcgctgac accgtctgct gcaaagactc acttttttaa
     3361 ttcaaggaga caccatgaac agtccgtcat cagatacggt ggcgctcgct gagcacgcat
     3421 accggattcg cagaaacgcc ttgctgatgg gcgaagtgca gggccagggc tatatcggcc
     3481 aggcactcga catcgccgat gtactcgcgg ttgcctactt cggcgcgatg cgctaccagc
     3541 ccgacaatcc cgaatgggaa ggccgcgacc gattcctgct gtccaacggc cactatgcga
     3601 ttgcactcta tgccgcgctg ttcgaagccg gcttcctgcc accggaagag ctcgaaacct
     3661 acggcagcga cgacagccgg ttgccgatgt ccggtatggc gagctacacg cccggcatgg
     3721 agatgtccgg cggctcgctt ggccacggcc tcacgatcgc ggtgggccgt tgtcttggac
     3781 tgaaacgcaa gggctcggac gcctttgtct acacgctctt ctccgatggc gaactcgatg
     3841 aaggtgcgat atgggaaggc cttatgtcgg cggctcactg gaagctggac aacctgatcg
     3901 ccatggtcga cgtcaacaac cagcaagctg acggtccctc ctcgaagatc atggcgttcg
     3961 aaccgctcgt cgagaagctc gaagcatttg gctggttcac gcagcgcgtg gacggcaacg
     4021 acattgacgc agtgaaggcg gccttcgatg ctgcacgcaa tcatccgaaa gagcagcccc
     4081 ggattatcgt gtgcgatacc agcatgggct gcggcgtgcc gttcctcgaa gaacgggaaa
     4141 agaaccattt catccgcgtc gatgcccatg agtggcaact ggcgctcgca gcgctcgaag
     4201 cagggagaca agcatgagca acaccaccac ccaaaagccg cgtctgaaga cttcggcgat
     4261 gattgcatcg atcgctggcg aaggacaggt cacgcgttct gctccgttcg gtcatgcgct
     4321 ggctcaactg gcacgcgaga agaagaacgt gatcggcatg accgccgacc tcggcaagta
     4381 caccgacctg cacatcttcg gcaaggagtt ccccgagcgt tactaccaga tgggtatggc
     4441 cgagcaactg ctgatgggtg cggcagccgg tatggcgcat gaaggtgcgc agccgttcgt
     4501 gacgacctac gccgtattcg caacccgtcg cgcgtatgac ttcatgcacc aggccatcgc
     4561 cgaggacaac ctcgacgtga agatcatctg cgcattgcct ggcctcacga cctgctacgg
     4621 cccgagccat caggcggccg aggatcttgc cctgatgcgg gcgatgccga acatgacggt
     4681 catcgatccg tgcgacgcac tcgacactga gcaaatggtg cctgcgattg ccgcgcataa
     4741 cggcccggtg tatgcgcgtc tgctgcgtgg caacgttccg gccgtgctcg acgaatacga
     4801 ttaccagttc gaactcggca aggccaagct gctgcgcgac ggcaccgaag ttctgatcat
     4861 ctcatcgggc atcatgacga tgcgttcgct ggaggtggcg aagttgctgg aagcggacaa
     4921 cgtgggagtt ggtgtattgc acgtgccgac catcaagccg ctcgacactg agacgatctt
     4981 gcgcgaagcc cggcgcacgg gtcgcatggt ggtggttgcc gagaatcact cggtgatcgg
     5041 cggccttggc gaagcggtcg cgaccacgct gcttacggcg ggcgtcacgc cgatgtaccg
     5101 tcaaatcgca ctgcccgatg aatttctcgg tgccggcgcg ttgccgacct tgcacgatcg
     5161 ctatggcatc tcgaccaacg tcatggccga tacaatcaag ggctggcttg gatgagcaca
     5221 cggtgtcatc cagacgctgc ggtacggcgg acatcgctcc gcctgcgtgt gaaacgagtc
     5281 gtctgctagc ttgagagggg gacggtgcgt cagtttcgtc gacgcaccgc taccgaaaaa
     5341 aacggaacgc tgacacttgt taagcaaagc ctccccgact gaagaagagc tctgaccggc
     5401 cgcttgcggc ttgtcttcgg cccccggaat gagcgagtga ctggcagcaa cgggtcgtcc
     5461 tgagtcggtc gcctcaggcg gagctcgggc cgtggttggc ctttagacgc tgccggcgtt
     5521 cggtctttga agcgaaagat cgacacagaa ccgcggtcat tctgatgtgt tgcggacagg
     5581 cgacgttagc tgcgtgccgc caccacgtcc ttcggggcac ggcgcccgcc agagcgcggt
     5641 agctgcgata agaaaacggc acgcgcggtg aggcaggagc ctcccagtgt ctgttcgaag
     5701 ccgcggctca ttgcgacgcg cgtgccgcct tagctttcaa tgacgtcgcc cgaccagatg
     5761 aaatcaaagg cagcaccggc ttcccgctgc accttccgga ccgtttccgc gtccggcgct
     5821 tcatactcac agatcatgtg ccggcgatcg atggaaacga cactgcgttt ccagacgatg
     5881 ccatgaagtt cgctgcacgg tgcgaggcgg tcctccacca cggacaactc cgcgtccgac
     5941 tgtggcgtcg gaaagctcct ttcaacgacg acgtgcgaca tgtcagccct cccatttctg
     6001 cacagtttgc attcgccaaa gattaatgcg gctgcgtttg aaggatcgtt gtaatgccgg
     6061 gatagcgatc gtaaagccgt tgcagtacgg cccgacttcg gggagcaagc ggcttgtcgc
     6121 ccagcaattc gcgcaacgaa gataaaaggc ggtcggaagc ctccgtcttg tcggcggcga
     6181 aagccgcctc gatcagcaaa gccgttgcaa cgattctctc ggtgggtgaa tcgagcgcgt
     6241 tggcagcctg cagcgcctga tgcccaaggg cgctcgcatc agcgctgcat cgacggtcga
     6301 gacagacacg cgccacctcg ttgagcgcat agcaaagatg ccgcttgtcg tcagctcgcc
     6361 gaagggcgtc aagaccttcg aacgcgaaca tgcctggggc aactgcgggc aggcgcagcg
     6421 ctgaaatctg actgatgacc tccgcaaagg gtgcgccgga gtcgcccatt ttctgcgcgg
     6481 ccgcgacgat ttcccccgcc agccgtgtaa cgtccgaata cgcgccccgc tcgaacgtca
     6541 tcgtcgcgag ccagacgaga cactggtact cgcgccagtg atcgccgatc gcgcgcgcct
     6601 gcgccacggc ttcgtcgagc ttcacgcatc cgatgtccag ttcgctgtcg gcccgtgcga
     6661 gcagcgcctc gccccacatc agttcgagga cgcgcaggtc gagatgttct gcgagcacgc
     6721 ccgcttcctg gaggacaccg cgcgcgcgag gcagatctcg ctccagttcg agcaaacagc
     6781 gacccgtgtt cgcgagctgc ttgcagcgcg tcgccgcatc ggctttgcgc gcggccattt
     6841 ctgcgcggat cgtcacctgg cgcgtgcgtt cgatgtcgtt cgcctgctgc gtcagccatg
     6901 acaggctatg gagtccctga accacgtcgg catgaagcga cagggtctcc gcgtcatgga
     6961 tagcctgttc gaattcctgc tccatcgacg cggcgcacgc ttcgcccgag ctcgcgacgg
     7021 caaccagccg cgcagtcagc aactggattt cgatctgaac ccgctcgctc ccgtgcggta
     7081 atgaacgcgc gtgagccagg ccgcggtccg cgagagtgcg cgcctccgtg gccgcaaaaa
     7141 cgcgaaggca atggttgctg gcttcaacgc atgctcgcgc tgtcatgcgc gagtcgcccc
     7201 ccaacgtggc gtggtgggcg agttctccat gcagccgcgg gtcatgcttg ctttcggcca
     7261 gcagttgccg cgcgatctga cgatgaatcg cacgcctgtg cggctgcgag agcgttcgat
     7321 aaacggcctg ccgcacgaga tcgtgtgcaa agtcgaactc cgtggatgtc gtctcagcca
     7381 ggattccacg acgctggagt tcggcaacgc acgacaggag ctcaggaagc gggcgatcga
     7441 gcagttgcgc aagctgctcg ggagcaaacg cacgaccgat cgatgccgcg aatgagagca
     7501 gatcacgcgt gggcccgtcg agtggcgcca gccggtcggc gatcaacgta tcgacggtac
     7561 acgtccacgg ttctctatcc atgcgatttg cgcgcgttag ctctagcagg taaagcgggt
     7621 tgccgccgct gtctgtcagc gcctgttcaa cgcggatgtc gctccccgat gcttcgataa
     7681 gcgtgcccac ttcgttagcg gaaagcggat gcagcggaac gtggatcagc ctgccgtcgc
     7741 gcgccagcga gtgcaacaga atccttgcgg accggttgtc ctccgtttcg gcgggacgtg
     7801 cagccgcgac gaagacaacg ggcagttcat ggtccagccg tcgcagcacg aagtgcaaca
     7861 gcgcggctga cgattcgtcg agccattgca gatcatccag cacgaacgcc aatcgatctt
     7921 cagcgcacag ccggccgagc agcccgacga ccgcttcaaa gaagcgcgcg cggcgtcgct
     7981 cgctgcgctg catttcaggc gttccaggcc cagcagggcg gataaaaggc gcgagatgtt
     8041 cgtcgacagg ctgtaacgca tcgattccga ggtcgcggag cgcgtctatc cagatgccgt
     8101 atggccggac agcctcggct tcatagcagc gactgcgcag cacgcggtac gacgcgttgg
     8161 acgcgcattg actgaagtag tccagcagcc gcgtcttgcc gatacccggc tcgcccgtca
     8221 gcagagtgag tccgtgcggc gtcgaagttg ccaacagctc atcgagtttc gcccgttctg
     8281 cgccgcgccc gacaagtggc aacggggcca tcggttgttg atcacgcgtc ggcgttcgtt
     8341 cgttttgccc tgctgcgcca gcgtcgtgtt tcgatgggcg gctctcgtcg ccaagtgcgc
     8401 aatggtcgta ctcgctcgtg tggcgcaacg aggtggtgtg gcgcacgcgc cggacagcat
     8461 catcgagcat cgcacacgcg cgcgcgccca tctcgcgatg gagaagctcc cgtgcatagc
     8521 ggtagtggca gtccccatcc gaacggcggc cggtcgcttc gagcaagctg atcaacgtcg
     8581 cgtgagccga ttcatcgagc ggatcggttt cgaccagctc acggccatac ctcaacgcaa
     8641 gcggcagatc gcacgagagc gccgcgatga gcgcgcgcag gaccgtcccg cgaatccgtc
     8701 caaaccgttc gcgctggccg acacaccagt tatggaaacg gtagcagccc ggaagttcga
     8761 gcccgccgag gaagtcacct cgtagcaact gcgctgcttc tgcgagcgcg gcaacaggca
     8821 atcgttcggt acccgatgca agcagcgcct gaatccggtt gctatcgacg aacgcgccgg
     8881 cggcatcgaa cgcaacatgt gtccggttgg cgacgatgcg ctcctgcgat gggtcgtcga
     8941 ccagcgggcg cagcttcgac agactccagc gaagcgcggc tcgcggatcg tcaggaccgt
     9001 cccagagcag atcgcagagg gccgatctgg gtattgcgcc gtccgacatc acgagatagc
     9061 ccagcagcgc gcgagtgcgg cgcgaagggg gcaacggtac gctcgcggca tcgcgccgca
     9121 cctccaactc gcctagcagg cgaatagtca aggggccgac gggagcgtca ggcgcatcgg
     9181 cgtttctcac gctggctcca accgtcaata caacgcttca gggggatgct catgtcgtcg
     9241 aacggcattc atcgaatgat cgcttgaatg caggttacta ccggagcgga acgctctgcc
     9301 acttgaaaat cgcgggaggc caacgtggca atccgtcaag gcaagctgaa tgagtacgtt
     9361 tttgcacttg tgcaccatcg caatccgatc cgaatcgatg cagcaccagt tgaaacaggc
     9421 acattccgac tgtatgaagc gctggctaac aatccgctat ctgcgcagcg tcttgcgaag
     9481 aagacgggtg ccgatgcgcg agcagtccgc gagtggttgg cggcgcaggc gacgcacggc
     9541 catctccaat acgatgcggc gcgccagcaa tactggatga ccgaacagca ggcgttcgcg
     9601 attagccgcg cacccggact ttcttctgcc gggacgcttt tcagatctgg cgtcgccgca
     9661 ccggtccatt cgcgcggtga tgcggacatg cggcggcagg cgcttcatcc gctgctcgta
     9721 ccgtttctga tgaaagccca aaagatgcag cggctgtgcc ttgaagtaat tgcacagcga
     9781 taagacgacg gaccgcagca aagcgttcgc aggcggctga ccgttgaatc tttgacaagg
     9841 atcatcgcga cgttcgtgat ggaatgccgg cgagggggcg gctacggcag acatcgggca
     9901 gtggtcggcc aggagcggta gctggcggct tgagacccaa tggccgttct cgctcttatt
     9961 tcggccgtta ccgagaagtc gcgcgcgtgc gaattctgtt tacttcaaaa gaccgtgcta
    10021 ccgttcctgg ccggagctcg tgtgatgctc cgaagcagtt ccatattgtt cgaacgtcag
    10081 cttcttcgac gaccacattt cccagatccc cgccgatatc gttccggacc gcctatccct
    10141 gcttggcggc gactgcggcg caggccttgc ggatcacgtc gagcaccgca tgcggctgcg
    10201 agagcatcgg gacgtggctg gtcttgagct cgacggtcgt ggcgcccatg cgctttgaga
    10261 cgaagcgttg caactcggga ttgaccgtgc ggtcctcggt ggccacgatg taccagctcg
    10321 gttttgactg ccaggcgata ccgtccagct tctggttgaa caggtcggcg ttcggcgcaa
    10381 agtgggttgc ataggcgatc tcctgctcat cagcagacaa atcgccggcg aagcacggga
    10441 cgccgctgcc gggcttcagc cagacgcggc cgtcagcgac gtcgatgttc tcgaagatat
    10501 cggtcgtggc gaacttctcc tgttggccct gcgacgtttc ggtctcgtcc ggggccaggg
    10561 ctgcgatgta gaccagagca gcaacccggg ggtccatgcc ggcgtgcgtg ataacggtgc
    10621 cgccgtagga gtggcctacc agcacgatcg gaccgctgac gcgtccgaat gtgcggatgg
    10681 tcgcgtcgac atcgcctttc acggtgtcca ggccgtattg ggccgccatc acctcgtgcc
    10741 cctccgctcg cagcgtcggg atcagcttct gaaaacagga accatcggcc cagatgccgt
    10801 gggagaaaac gatactcggc ttcatagtgc tctccttccg tgagtgcttg ctggcgcttg
    10861 cgggggcttg cggcgaccgc agtgactgcg gcagccaggc tgaggccgac gacgccgacc
    10921 agaacatcgc gacgggactc gcttaatttc ccgacaccga cctgttcagc ccgaagggct
    10981 cactccaaca gtgggtcaga attcggtgga aatcaaccgc cgctggcatc gctgcctgcg
    11041 cagcgacagg ctgtcgccct cggcacgttc cctccctgat tccccagacg cgtggagaat
    11101 cagtgctgag ttcaatgggt aagcagaagg ttgggacttg gtcatcccca cttgcagcta
    11161 gaacgccaac accgcaacct cgtcgtcctc tcgcctctgc gactcgcttt gtaactcgcc
    11221 ctcgccacgt cggattcagt ggaaatttct gctcgccgtc ggcagttccc cgagcaggtc
    11281 cgacacgtca acaagccgtt gtgcaacgag atgcttcacg tcgccctggc gctgccacac
    11341 gccgtagacc gcaagcaatg acgcgccgag cgcctcgcgc cgctgccgct cgagcaccga
    11401 ctgccagatg atcacgttga cggaccccgt ctcgtcctcg agggtcatga acatgacgcc
    11461 gttcgccgtg ccggggcgct gacgtaccgt gacaattccg caggcccgcg cgagctggcc
    11521 gtcgcggtag cgcatcagcg cttcggcggg catcaggcgc atcgacagca gcttcttgcg
    11581 cagcagggcg agtgggtggc ggccgagcgt caggccggtc gaacggtagt cggcgatcag
    11641 gtcctgtcct tccgtcggtg ccgtcagcgc cggcgcgtct tcctcgcgcg atgtcgcgcg
    11701 caacaggtcc ttgtccggga cggccgcgac ggcggcccac agcgcctgcc gccggtcacc
    11761 ggcgagcggt ttcaatgcat tggcggccgc gagcacctgc aggtcatggc gatccagctg
    11821 tgagcggttc gcgagatccg cgacatcgcg gaacggccgc accgcacgcg ccgcttcgat
    11881 gcgccgcgcg gtttcctcac gcatgccacg gatcacggac atgccgagac gcaccggcgc
    11941 catgctgcct tcgccttcca tcgttgaatc ccagccgctg atggtcacgt cgacgggcag
    12001 cacgccgacg ccgtggcgct ttgcatcctg aaccagttgc gacggcgagt agaagcccat
    12061 tggctggcta ttgagcatcg cgcacaggaa cgcggccggc tcatggcgct tcagccagca
    12121 gcttgcgtag acgagcagcg cgaagctcgc cgcgtggctt tcgggaaagc cgtattcgcc
    12181 aaatccctgg atctgcgcaa agatcgattc ggcgaactcg cggtcgtagc cgcgctggag
    12241 catcccttca acgatgcggt cgtgatagtg ctgcagtccg cccttgcgct tccatgcggc
    12301 catcgcgcgg cgcagctggt ccgcctcgcc ggcgctgaaa ccggccgcga gcattgcgac
    12361 ctgcatcacc tgctcctgaa agatcggcac gccgagcgtg cgcttcagcg cctgttcgac
    12421 gtccggactc ggatagctga caggttccag tccctgccgc cggcgaaggt acggatggac
    12481 catgccgccc tgcaccgggc ccggacgcac gatcgcgacc tcgatcacga ggtcgtagaa
    12541 cttccgtggc ttaagacgcg gaagcatgct catctgtgcg cgtgactcaa tctggaacac
    12601 gccgacggta tcggccgcac agatcatgtc gtacgtcgcc tcgtcctcgg gcggaatgtc
    12661 ctgcatctcg aacggctcgc cgcgctgctg cgacaccagt tcgagcgtgc ggcggatcgc
    12721 cgacagcatg ccgagcgcga gcacgtcgat tttcagcagg ccgagcgctt ccagatcgtc
    12781 cttgtcccac tggatcacgg tgcgatcctg catcgacgca ttttcgaccg gcacgagccg
    12841 cgagagcttg cctcccgcga tcacgaagcc gcccgtgtgc agtgaaagat gtcgcggaaa
    12901 gcccagtagc tgagcggcca gcgtcgccca ttgctggatc atcggtgtgt ccggatccag
    12961 cccactctca acgaagcgac ggataaggtc agcgctcgaa tcgaaccagt gatgctcctt
    13021 tgcgacgcga tcgacgatca tcggatcgat gcccagcgcc ttgccggaat cccggattgc
    13081 agagcgaggc cgatatgtcg tcaccgcggc tgcgagcgcc gcgcgggtcc gcgtgtattt
    13141 acggtagatg tactggatga cctcttcccg tctctggtgc tcaaaatcga cgtcgatgtc
    13201 cgggggttcc ccgcgctctt tactgatgaa gcgctcgaaa agcatgctgc ttctatcggg
    13261 atcaacctct gtgacgccca ggcaaaagca gactgcggaa ttggccgccg agccgcgccc
    13321 ctgacaaagg attccctcgt tgcgcgcaaa gcgcacgatg tcgtacaccg tcaggaagta
    13381 tggctcgtat ttcaggtcgg cgatcagcga cagttcgtgc tcgatctgct tctgaacgct
    13441 gtgcggaatg ccgcgcggat agcgacgctg ggcgcccaga taggtttcgc gtcgcaggta
    13501 ggcggtcggc gtggtgccgg gtggcacgag ttcgtcggga tactcgtatc gaagctcgtc
    13561 gagcatgaac gtgcagcgcg acgcgatgtt cagcgtctgg gcgagcgcgt ccgccggata
    13621 gacgtttgcc agccgcaacc gcgagcgaag atgctgttcg gcgttcggcg tcagctcaaa
    13681 cccgcattcg gtcaccggcc ggttcaggcg gatcgccgtc agcgtgtcct gcaacggctt
    13741 gcgtgagcgc acgtgcatca acacctggcc ggtggccacc acgttcaatc catgccgctc
    13801 ggcgacgtcg tagacggtat tgcgatgcag gtcatcgagt gcgcgggcgt gcagcgtgaa
    13861 ccccatccag cagcggtctg tgccgaacgt ctgtgccgcc cattgcgcct gcgcgtcgag
    13921 gcgtgaatcc ttcgcgggaa attcgggcgt caggatgatc agacaatctg gcagcgcgcg
    13981 cagatgcgtg tagggcgaat cgggatgatc gagatcgtgc ggatagacga catacgtccc
    14041 cttggccgtt cgggtgcgcg cgagcgtgat cagttccgac aggttgccgt agccgtcgcg
    14101 gttctgggcg atcgcggtga aggcgagggc aggtgagccg tccgcgttcg tgacgcggaa
    14161 gtgcgcccca accagcagct tgatgcccgt ttccttcgcg gcgacgtgcg cgcgcacgat
    14221 gcccgcaagc gagcactcgt cggtgatcgc aattgcctcg tacccaagct gcgccgcgcg
    14281 cgtggccagt tcctcggcgt gcgacgcgcc ctgcaggaac gtgaagttgg acgcgcactg
    14341 caactcggca tagccgggca ggccgaacga agcgggcagc atggcttatc cgaacagccc
    14401 atgcaggtac cagcgcggct cgtcttcgtc gacggcgcca atccgctcgc ggtagataca
    14461 gtagcagatg tagtcgtcgg cctcggcaac gaagtagtcg cgcgtcaccg gctcgccccg
    14521 gaaccagccc gcttcgacgc gttcaccgga tgacactgtg cgcagtggcg agccgtaaaa
    14581 tggtcgctcg ccgcgcatca gcaacttgat cggcgattcc agcagccagg cgggacgcgg
    14641 cgtgtccgtc gccggagcta cgtacttcgt cttgtccgtg acgggcaccc agcgggcggc
    14701 gacttcaggg cggtgatcgg caacgggcgc gggcttcagc acgttttcgg caccgaggcg
    14761 tgcgaccagc agttcgagca gccgcgcgtg atcggcggcc gtaccgcccg gctccggaaa
    14821 cagcgagtcc gacgcgggct ccgcctcctt cacttcctgc acgacgagct gcagcgcgat
    14881 gacggcggcc gcgagttcga cacggcccag ccgttctctc agcagtcgca tcaggtgatc
    14941 ctcgcgccac gtcggttctg ctagcgatac ctcgattgtc gtcgggtcga tcgcgtcccg
    15001 acctcgttcg tgctcgagca gcagcgcgat gcgcgtgacg gccagctgct tctgtgacag
    15061 ccagcccgtt agctggacga cgagccggcg ggccgcaaaa acacacgctt cagcgtgctc
    15121 gatgcgatcg ggcagttcga cgcgcgcctg aaaggtcggc ggcgtttcaa accattcata
    15181 cacttcgggt gcgactccat tggcgcggtc gagcacatcg agcagtgccg tgccgcagcg
    15241 ttttttcagt cccgcacggg gcaggcgcat aagctgtccg actgtctcgc agccgatgcc
    15301 cgtcagccag tcgaggtggc gtcgggcggg cgtgggcagg ttgaccggca gtccggcgag
    15361 ggcgcgtgac agcgaggcat gcgacagcgc aacgccgccg cagtaccgcg cgagcagcca
    15421 tgccgcctgt cccgttggtg cgacgctgat cgtggcagta acacccatcg cgtcggcgac
    15481 ccggcgcgcc atgcgacgca ccggccggat gccgccaaag agccgcagac tcgccgacac
    15541 gtcgacgagc accgtgcttt cctcggcgat cacgacctgc ggtgagaggt tcaacagtcc
    15601 tgtcgcgatc tcgcgcaccg cgttcacctc tgccgtgctg ttgcgctcct gcatgacggt
    15661 ggcaggcgcg agcgtcagga caccgccgcg acgcatgccg atgcagacgc ccgccgcgcg
    15721 cgcggacggg tcggcggcaa cgacgcgctc cttttcgagc acggcaaggc ctgcgtcgtc
    15781 atcgggttca agcgaccact tcggccggaa cacttcgagg cacaacgacg ggagatggac
    15841 gcctatccac agttgcatgg cgttttacgt tcaggatcgg ggagggcgtc agggacacga
    15901 acagcggttc atcgcgggcg gggccgcggc gcttcagaaa ctggacatcg acgccgcctt
    15961 tcgccgcgcg gatcgcaaat cgcagtggtg cgggcgacgc gtcacgtgtc gtcgccagcg
    16021 gccgcaccat gaaaaagagc gactcggacg tttgcgcggc caggtgcagc cggcgcaccg
    16081 cctcaggccg cacgtgctgc tgccagaaca cgagcgcgcc gcaggtgccc gcgcgcagaa
    16141 tctgctcggc ggcccacaac gcgtcggcag tcttcgatgc cttcaccgtc agcagaatgg
    16201 acgggtcaag gccccaccac gcgaacgcgg tcggctggac gttgtgcggc ggctgcagga
    16261 gcacgactgg tttggatccc aggcgctcga aggcgggtcg cagcagacgc agttcgccgc
    16321 taccggtctg ctggacgagc agatcggtca gcgcgccgcg tggccagccg ccaccgggca
    16381 gttccgccgc aagcgcttcg tcgccgcaag ggacgacccg ggatgcggcg cgggcaagct
    16441 ggttgccctt ccacaaatcg ggatggatcg cttcgggtag catgacggcg ctcatcgctc
    16501 tccccagaaa acactgtata tataaacagt agtatagcct ggtaaggcca cggtcatcgt
    16561 cggttttgtc ggtaaacgga attcgttgga ctagcgcgca accagctaga ctcggaagca
    16621 ctgaattgac tggactgccg ggaaagcgcg gaaatccaag ggctgccgcg cggcgcaggt
    16681 ggcccctcgc aacatgccca cgcggcttac gttcagaaag ggcgagtggc gcttcacgac
    16741 ccctatgctc aggtcacaac tatgcgcagc taatcggtcg ctgatggtgg cgacccatgc
    16801 atgacgaggc tttggcaagg tgagcaagca aggatctgga catagtttcg acctgtgatg
    16861 cagtggtggt cccacttctg gagatcacca tggacatcgt tcgccgctcc caccgtactg
    16921 acataagcgg tttcgccgac gggcggatcg ggccatacat tgctgcgttc aaacagtacc
    16981 tgagccaacg ccggtacgct gcaaagacgg tcgctactta cctgctgtgt atcacgcact
    17041 tcgcccaatg ggcagactcc gggccctgtc ggcggcg
//
LOCUS       DATASH010000889         4439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_151072,
            whole genome shotgun sequence.
ACCESSION   DATASH010000889 DATASH010000000
VERSION     DATASH010000889.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151072"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(428..1108)
                     /locus_tag="VHV99_23790"
     CDS             complement(428..1108)
                     /locus_tag="VHV99_23790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX3638441.1"
                     /translation="MTLLQTARRSSAPRETATASLAEQAYAFVKREIITMRLRPSEVL
                     NEAELMVLTGIGRTPVHQALHRLVHEGMLTIMPRKGIMVRPVSLDDVLAIIDVRLVNE
                     SYCVELAARHAQQHDFDAMQALLDRSSVCAAAHDIEGMMDIDREFHLAISAASRNPVL
                     ADILRGLHERSLRFWFISLSEPHHLEDVHEEHLELFRLLRERDAEGARQSVQKHIEAF
                     RATLFNRI"
     gene            complement(1314..2666)
                     /locus_tag="VHV99_23795"
     CDS             complement(1314..2666)
                     /locus_tag="VHV99_23795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3638442.1"
                     /translation="MNPPSLSANADASSDAWLEAAALRKITWRIMPFLFLVYVLSYLD
                     RVNIGYAKLQFTGDLGLSNAAYGLGAGIFFFGYFVFEVPSNLLLRKFGARATIARITM
                     LWGLLSCLMMFVRSETMFYVLRFFLGVAEAGLVPGVVLYLTFWFPSDRRARMVAVFMA
                     AIPVAGIIGAPLSGFLMSALHEAHGLRGWQWMFLIEGIPSILAGFWALAVLRNTPAEA
                     AWLTDDEKRVILGRLERENSAAAAAGAEHSLAVALRSGRFWLLTLIYFCLVTGNAGFS
                     FWLPQIVKDLGVTNLVTNGFVTAIPYLAAGIGMILIGRSSDITGERRWHYAVCCFIGA
                     AGLLGSASVTNSIPLAVTGLSIAYIGILAGFGIFWSMSTTFLQGTAAVAGIAVINSIA
                     NLAGYVSPYVLGIVKDATHSVTFGLVLIAGALIVGGLVTLMMPRVKVQHAGAVTPSNA
                     "
     gene            complement(2674..3363)
                     /locus_tag="VHV99_23800"
     CDS             complement(2674..3363)
                     /locus_tag="VHV99_23800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2848 domain-containing protein"
                     /protein_id="HEX3638443.1"
                     /translation="MQQVSFNVVDRSGDTRADIVEVNRLTIAGWAGRNQAAIEHHIAE
                     LAELGVKRPSTTPCFYRLGAELLTQAGQIDVVGVKSSGEAECVLVRSQAGLLVTVGSD
                     HTDREVEAYGVTVSKQICPKPVARDAWRFDDVAGHWDQLELRAFAVTNNGERRIYQQG
                     SVASLLPAADLLERAPVAEGAAMFCGTLAVQGGIVGMADGDALELELHDPILNRTLRH
                     AYRVHALPIVE"
     gene            3574..>4439
                     /locus_tag="VHV99_23805"
     CDS             3574..>4439
                     /locus_tag="VHV99_23805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX3638444.1"
                     /translation="MHHGIGFIQDLAVVMALAGVVTVLFHRLKQPVVLGYIAAGVIIG
                     PYTPPFQLIHDEQTIQTLGELGVVFLMFSLGLEFSLRKLFKVGATAIVAALSEIVLML
                     WIGYEIGSAFGWSSMDSLFLGAILAISSTTIIVKALSELGLKRESFAQLVFGILIVED
                     ILAIAMLVLLTGIAQTGELSAGVAVVTLGKLLLFMTVSLVVGILVVPRALNYVARARS
                     DEMLLVSVLGFCFGFCLLVVKLDYSIALGAFLIGAIMAESRHLHRIEHLIGPLRDAFS
                     AIFFVTIGLMLNP"
BASE COUNT          853 a         1320 c         1456 g          810 t
ORIGIN      
        1 atccgttcga gcgcggtttc gacgagcgcg cggctcgtgg tgcggccggc ggcgaggtcg
       61 gcggcaagct gggctagcgg cgggaagggc gtgaattcag tggccatggt gtcggtgtcg
      121 ggaagaatcc ggttgaacag agcgaagccg gttaaaggcg attggttaaa ggcgattaac
      181 tgtggaagat tatgcgttcg atgagatgcg ttggctgaca gggcgttcga taagttgcgt
      241 tgggtcagat gcggtggacg cagaggcgtt ggctgagagg cgttcgatag gttgcgttgg
      301 ctcagatgtg gtcgacgagt tgcgttggct cagatgcgtt cgatgaattg cgttggctga
      361 gaggcggtcg atgagttgcg ttggctgaga ggcggtcgat gagttgcatt ggctcaaatg
      421 cggccggtca aatacggttg aacagcgtcg cgcggaacgc ttcgatatgc ttttgcaccg
      481 attgccgggc gccttcggca tcgcgttcgc gcagcaggcg aaacagctcc agatgttctt
      541 cgtgcacgtc ttcgagatga tgcggctcgg acagcgaaat gaaccagaag cgcagcgagc
      601 gttcatgcag cccgcgcaga atgtcggcca gcaccggatt gcgcgacgcc gccgaaatcg
      661 ccagatgaaa ttcgcggtcg atatccatca tgccttcgat atcgtgcgcg gccgcgcata
      721 ccgacgagcg gtcgaggagt gcctgcatcg cgtcgaaatc gtgctgctgc gcgtggcgcg
      781 cggccagctc gacgcagtag ctttcgttga cgagccgcac gtcgatgatc gccagcacat
      841 cgtcgagcga aaccgggcga accatgatgc cctttctcgg catgatggtg agcatgcctt
      901 cgtgcaccag ccggtgcagc gcctgatgca ccggcgttct gccaatgccg gtcagcacca
      961 tcaattccgc ttcgttgagc acctcgcttg gacgcaagcg catcgtgatg atctcgcgct
     1021 tgacgaacgc ataggcttgt tcggcgagac tcgccgtggc ggtctcgcga ggggcactgg
     1081 aacggcgggc agtctgcaaa agcgtcatgt gaagaggaag cgggcggtgt cgtccgcatt
     1141 gtagagcaag gttttgcgcg gacgacggct ggcgggcggt tgctcaccgg acgctgtccg
     1201 tgcgcgatgg caaacaggca aggcaatcac gcaagcagcc gaccacgcaa ccacacaacc
     1261 acagcgacta cggcaaccac agcgactaca gcaaccacag caaccacagc aaatcaggcg
     1321 ttgctcggcg tcaccgcccc agcgtgctgc actttcacac gcggcatcat caacgtgacc
     1381 aacccgccga cgatcaacgc gcccgcgatc agcaccagcc cgaacgtcac gctatgcgtc
     1441 gcatccttga cgatgccaag cacatacggg ctcacatagc cggccaggtt cgcaatcgag
     1501 ttgatcacgg caatgccggc cacggccgcg gtgccctgca ggaacgtggt ggacatcgac
     1561 cagaagatgc cgaagcccgc cagaatgccg atgtacgcga tcgacaaacc ggtaacggcg
     1621 agcggaatcg aattggtcac cgaggcgctg ccgagcaaac ccgccgcgcc gatgaagcag
     1681 cacacggcat agtgccaacg gcgttcgccg gtgatatccg acgagcggcc gatcaggatc
     1741 atgccgatgc ccgccgccag ataaggaatc gcggtaacga agccgttcgt gacgagattg
     1801 gtgacgccga ggtccttgac gatctgcggc agccagaacg agaagcccgc gttgccggtg
     1861 acgaggcaga agtagatcag cgtcagcagc cagaagcggc ccgagcgcaa ggcaaccgcc
     1921 aggctgtgtt cagcgccggc cgccgctgcc gcggagtttt cgcgctcgag acggccgaga
     1981 atcacgcgtt tttcgtcgtc ggtgagccag gcggcttcgg cgggcgtgtt gcgcaacacg
     2041 gcgagcgccc agaagccggc gaggatcgac ggaatgccct cgatcaggaa catccactgc
     2101 cagccgcgca agccgtgcgc ttcatgcagc gccgacatca gaaagcccga taacggcgcg
     2161 ccgatgatgc ccgccaccgg aatcgccgcc atgaacacgg cgaccatccg cgcgcgccgg
     2221 tcggacggaa accagaaggt cagatacagc accacgcccg gcaccagccc ggcctcggcg
     2281 acgccgagga aaaagcgcag cacgtagaac atcgtttccg agcgcacgaa catcatcaga
     2341 cacgacagca gcccccacag catcgtgatg cgggcgatgg tggcgcgcgc gccgaacttc
     2401 ctgagcagca gattgctcgg cacttcgaag acgaaatagc cgaagaaaaa aatccccgca
     2461 ccgagtccgt acgccgcgtt cgagaggccg aggtcgccgg tgaactgcag cttcgcataa
     2521 ccgatgttga cgcggtccag gtacgacagc acgtagacga gaaacaggaa cggcatgatg
     2581 cgccaggtga tcttgcgcag cgcggcggct tcgagccagg cgtcggaaga cgcgtcggcg
     2641 ttggcggaga gcgaaggggg attcatgcgt gcctcattcg acgataggta gtgcgtgcac
     2701 gcggtaggcg tggcgcagcg tgcggttcag gatggggtcg tgcagttcga gttcgagcgc
     2761 gtcgccgtcg gccatgccga cgatgccgcc ttgtacagcc agcgtgccgc agaacatcgc
     2821 ggcgccctcg gcgacgggcg cgcgctccag cagatccgct gccggcagca gcgaggcgac
     2881 gctgccttgc tgataaatgc gccgctcgcc gttgtttgtc acagcgaagg cgcgcagttc
     2941 aagctgatcc cagtgaccgg cgacatcgtc gaaacgccag gcgtcgcggg cgaccggttt
     3001 cgggcagatc tgcttggaca cggtgacgcc gtaggcctcg acttcgcggt cggtatggtc
     3061 ggagccgacg gtgacgagca gtcccgcctg cgagcgcacc agcacgcact ccgcttcgcc
     3121 gctggatttc acgccgacca cgtcgatctg cccggcctgc gtcagcagct cggcgccgag
     3181 gcggtagaag cagggggtgg tcgacggccg cttcacgccg agttcggcca gttcggcgat
     3241 gtgatgctcg atcgccgcct ggttgcgccc ggcccagccg gcgatcgtca gacgattgac
     3301 ttcgacgata tcggcgcgcg tgtcgccaga acgatctact acattgaagg acacctgctg
     3361 catcactcga ctccaaagga aaatatgcgg caaatattat tgtgatattt cacaggtgtc
     3421 cagcgtgtca aaaatgtttc ggctcgagcc tcaaggcgtg cggcttgtga tccagccaat
     3481 cccaagccct ttcgacgttt gcgcgagtcg gcgcgtcgcc cctgtgcgtt acactgcgtt
     3541 tttgagacat tactttttgt aagggacaca tcaatgcatc acggcatcgg cttcattcag
     3601 gatctggcgg tcgtgatggc gctcgccggc gtcgtcaccg tgctgttcca tcgcctgaaa
     3661 cagccggtgg tgctggggta catcgccgcg ggcgtgatca tcgggccgta cacgccaccg
     3721 ttccagctga tccacgacga gcagaccatc cagacgctcg gcgaactcgg ggtggtgttc
     3781 ctgatgtttt cgcttgggct tgaattcagc ctgcgcaagc tgttcaaagt gggcgcgacg
     3841 gcgatcgtcg cggcgctttc cgaaatcgtg ctgatgctgt ggatcggtta cgagatcggc
     3901 agcgcgttcg gctggagttc gatggactcg ctgttcctcg gcgcgattct cgcgatctcg
     3961 tcgacgacca tcatcgtcaa ggcgctgtcc gagctgggcc tgaagcgcga gagctttgcg
     4021 caactggtgt tcgggattct gatcgtcgag gacattctcg ccatcgcgat gctggtgctg
     4081 ctcaccggca ttgcgcagac aggcgagttg agtgcgggtg tcgccgtggt gacgctcggc
     4141 aagctgctgc tcttcatgac ggtgtcgctg gtggtcggca ttctggtcgt gccgcgtgcg
     4201 ttgaactacg tggcgcgcgc caggagcgac gaaatgctgc tggtgtcggt gctcggcttc
     4261 tgcttcggct tctgtctgct ggtggtgaag ctcgattaca gcattgcgct cggcgcgttc
     4321 ctgatcggcg cgatcatggc cgagtcgcgc catctgcatc ggatcgagca tctgatcggg
     4381 cccctgcgcg acgccttctc cgcgatcttc ttcgtgacga tcggcttgat gctgaaccc
//
LOCUS       DATASH010000890         2014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5692398,
            whole genome shotgun sequence.
ACCESSION   DATASH010000890 DATASH010000000
VERSION     DATASH010000890.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5692398"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..670
                     /locus_tag="VHV99_23810"
     CDS             2..670
                     /locus_tag="VHV99_23810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX3638445.1"
                     /translation="MICDCDGVLIDSEAVAARMLVHELEARWPDADVEPVVLPLLGLR
                     IEKVLQGTAAQLGKRLSAEDIDAIRRAVEAAAMQAPTVEGIETALAQVPLTKACASNS
                     FRPYVETVLARTGLVKFFGDRLFCADAVPNPKPAPDVYLAAAHAFGLAPSACLVVEDS
                     VTGVTAAAAAGMTVLGFIGGGHASDAQIDALHAAGARHVFDDMLQLPELVAQWTLSAT
                     AATP"
     gene            838..1947
                     /gene="ugpC"
                     /locus_tag="VHV99_23815"
     CDS             838..1947
                     /gene="ugpC"
                     /locus_tag="VHV99_23815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEX3638446.1"
                     /translation="MASVTLRNIRKAYDDNEVMRDINLDILDGEFVVFVGPSGCGKST
                     LMRMIAGLEDISGGDLTIDGTRVNDVPPAKRGIAMVFQSYALYPHMTLYDNMAFGLKL
                     AGTKKPEVDAAVRNAAKILHIDHLLDRKPKQLSGGQRQRVAIGRAITRKPKVFLFDEP
                     LSNLDAALRVKMRLEFARLHDELKTTMIYVTHDQVEAMTLADKIVVLSAGNVEQVGSP
                     TMLYHAPANRFVAGFIGSPKMNFMDGVVQSVTHDGVTVRYETGETQRVAVEPGAVKQG
                     DKVTVGIRPEHLHVGSTEDGISARTMALESLGDAAYLYAESSVSPDGLIARIPPLERH
                     AKGEMQKLGATPDHCHLFDSEGKAFQRKIVEVLAA"
BASE COUNT          404 a          613 c          677 g          320 t
ORIGIN      
        1 gctgatctgc gattgcgacg gcgtgctgat cgacagcgaa gccgtggcgg cgcgcatgct
       61 ggtgcacgaa ctcgaagcgc gctggcccga tgccgatgtc gaaccggtgg tgctgccgct
      121 gctcggcctg cgcatcgaaa aggtcctgca aggcacggcg gcgcaactcg gcaagcgcct
      181 gagtgccgag gatatcgatg cgatccgtcg cgcggtggag gcggcggcga tgcaggcgcc
      241 cacagtcgaa ggaatcgaga ccgcgctcgc gcaggtgccg ctcaccaaag catgtgcgag
      301 caacagtttc cggccgtatg tggagacggt gctggcgcgc acgggccttg tgaagttttt
      361 cggcgaccgg ctgttttgcg ccgacgcggt gccgaatccg aagcccgcgc ccgatgtcta
      421 tcttgccgcg gcgcatgcct tcggcctcgc gccgtccgct tgcctcgtgg tggaagacag
      481 cgtgacaggc gtgacggccg cggccgcggc cggcatgacg gtgctgggct tcatcggcgg
      541 cggtcatgcg agtgacgcgc agatcgacgc gttgcatgca gcgggcgcgc gtcatgtatt
      601 cgacgacatg ttgcagttgc cggaactggt cgcgcaatgg acgctgagcg cgacggcagc
      661 gacgccgtaa gcgcggcagt tcaaagcaga tgggcggcga atgagcagga tctgaagcgc
      721 acatcgggaa gggaatcaac gcagctgctg aagaaacgga agcaccgaaa gcaccctgag
      781 ccactgacac agctgaagca ctggatagca acacacagca atacacggag acacatcatg
      841 gcaagcgtaa ctctgcgcaa catcagaaaa gcctacgacg acaacgaggt gatgcgcgac
      901 atcaacctcg acatcctgga cggcgaattc gtcgtgttcg tgggcccgag cggttgcggt
      961 aaatcgacgc tgatgcggat gatcgccggc ctcgaagata tcagcggcgg cgacctgact
     1021 atcgacggca cgcgcgtgaa cgacgtgccg cccgccaagc gcggcatcgc gatggtgttc
     1081 cagtcgtacg cgctgtatcc gcacatgacg ctgtacgaca acatggcgtt cggcctgaaa
     1141 ctcgccggca ccaagaagcc ggaggtcgac gccgccgtgc gcaacgcggc gaagatcctg
     1201 cacatcgacc atctgctcga tcgcaagccg aagcagctct cgggcggcca gcgccagcgc
     1261 gtcgccatcg gccgcgcgat tacgcgcaag ccgaaggtgt tcctgttcga tgaaccgcta
     1321 tcgaatctcg acgccgcgct acgggtgaag atgcgcctcg aattcgcgcg tctgcacgac
     1381 gagctgaaga ccaccatgat ctacgtgacg cacgaccagg tcgaagccat gacgctggcc
     1441 gacaagatcg tggtgctgtc ggcgggcaat gtggagcagg tcggcagccc gaccatgctt
     1501 tatcacgcgc cggccaatcg cttcgtcgcc ggttttatcg gctcgccgaa gatgaacttc
     1561 atggacggcg tggtgcaatc ggtcacgcac gacggcgtca cggtgcgcta cgaaaccggc
     1621 gagacgcagc gtgtcgcggt ggaaccgggc gcggtgaagc agggcgacaa ggtgacggtc
     1681 gggattcgcc ccgagcattt gcacgtaggc tcgacggaag acggcatctc ggcgcgcacc
     1741 atggcgctcg aatcgctcgg cgacgccgcc tatctgtatg cggaatcgag cgtttcgccg
     1801 gatggcctga tcgcgcggat tccgccgctc gaacggcacg ccaagggcga gatgcaaaag
     1861 ctcggcgcca cgccggatca ctgccatttg ttcgacagcg aagggaaggc attccagcgc
     1921 aagatcgtcg aagtgctggc cgcgtgatga agtgatcagg aaagcggcgc gcgcgccgct
     1981 ttccgcgcaa tcacattgcg atcggtaccg cggt
//
LOCUS       DATASH010000891         2483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_942817,
            whole genome shotgun sequence.
ACCESSION   DATASH010000891 DATASH010000000
VERSION     DATASH010000891.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942817"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..403
                     /locus_tag="VHV99_23820"
     CDS             2..403
                     /locus_tag="VHV99_23820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007745312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3638447.1"
                     /translation="MQLDHATIVTAELETARRFFVDVAGLTEGARPPFSIDGYWLYAN
                     GRPVIHLIDATVPAVAGRAAPRIDHIAFRLESADEWRALLQRLRVAQTPYQLAQVPPI
                     GPQRAELQLFVALAPGVVIEFVTALRHAQRN"
     gene            436..1602
                     /locus_tag="VHV99_23825"
     CDS             436..1602
                     /locus_tag="VHV99_23825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3638448.1"
                     /translation="MPIPLLALAISAFAIGTTEFVIMGLLPEVARDLAVSIPSAGLLV
                     SGYALGVAVGAPLLAVITSRMPRKLALQLLMGVFIVGNTLCAIASSYSLLMIARVVTS
                     FAHGSFFGIGAVVAASLVPAEKRASAIAMMFTGLTLANVLGVPFGTFVGQQFGWRAAF
                     WIISAFGVLSLAGVTALVPNRHDSGAVGLGHEVRVLKDPQVWTALAMTVLGFGGVFVV
                     FTYIAPILEQISGFSPRGVTLILVLFGVGLTIGNTVGGKLADRALMPSLMGILVALTV
                     VMAIFARTSHSQVAAAITIFVWGIAAFATVPPLQMRVVEKAAAAPNLASTLNIGAFNV
                     GNAGGAWLGGLVIDHGHSLDTLPWVAAAVSVAALLLTWFAARMDAPASTVAQGA"
     gene            1791..2480
                     /locus_tag="VHV99_23830"
     CDS             1791..2480
                     /locus_tag="VHV99_23830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEX3638449.1"
                     /translation="MHSPLALVRDPTADTDAPSRATEPFDALEHLVGVNLARLRAERQ
                     LSLDALARASGVSRAMLAQIESARSVPSIKVLCKVAAALKVSVAAFLRRHATNGFEHL
                     PAERSSRLVSSNGRYSARPLYPDAEPTAAEFHELRIAPLHTEAGTRRAPGTTVNLVVS
                     EGTLEVSVHDQRQLLATGDAIVFDADQPHSLRNPGDTEARAFRVTLKAETPPRWDVPA
                     PHDLTRQAAPV"
BASE COUNT          368 a          770 c          834 g          511 t
ORIGIN      
        1 tatgcaactc gatcacgcaa cgatcgtcac cgccgagctc gagaccgccc gacgtttctt
       61 cgtcgacgtc gccggattga cggagggcgc ccgtccgccg ttttcgatcg atggctactg
      121 gctgtatgcg aacggccgcc cggtgattca tctgatcgac gcgacggtgc ccgccgtagc
      181 gggcagggcc gcaccgcgca tcgatcacat cgctttccgg ctggagagcg ccgacgaatg
      241 gcgggcgcta ttgcagcgct tgcgtgtggc gcagacgcct tatcagctcg cgcaagtgcc
      301 accgataggg ccgcagcgag ccgaattgca actgttcgtc gcgctcgcgc ccggggtggt
      361 catcgaattc gtaaccgcgc tgcgccacgc tcaacgcaac tgacacgctc aactttaaaa
      421 cctggagtag aaaaaatgcc cattccgtta ctggcgctag cgatcagcgc tttcgccatc
      481 ggcacgaccg agttcgtgat catgggcttg ctgcccgagg tcgcacgcga cctggccgtg
      541 tcgattccgt cggccggttt gctggtgagc ggctatgcgc tcggcgtggc ggtcggcgca
      601 ccgctgctcg cggtgatcac cagcaggatg ccgcgcaagc tcgcgttgca gttgctgatg
      661 ggcgtgttca tcgtcggcaa tacgctgtgc gcgatcgcgt ccagctattc cctgctgatg
      721 atcgcgcgcg tggtgacgtc gttcgcgcat ggctcgttct tcggcatcgg cgcggtggtg
      781 gcggcttcgc tcgtgccggc tgaaaagcgc gcgagcgcga ttgcgatgat gttcaccggc
      841 ttgacgctgg cgaacgtgct gggcgtgccg ttcggtactt tcgtcggcca gcagttcggc
      901 tggcgtgcgg cgttctggat cattagcgcg ttcggcgtgc tgtcgctcgc aggcgtgact
      961 gcgctggtgc cgaaccgtca cgattccggt gcggtcggat tgggccacga agtgcgcgtg
     1021 ctgaaggacc cgcaagtctg gaccgcgctc gcgatgacgg tgctcggctt tggcggcgta
     1081 tttgtcgtat tcacctatat cgcaccgatt ctcgagcaga tcagcggctt ctcgccgcgt
     1141 ggcgtgacgc tgattctggt gttgttcggc gtgggcctca caatcggcaa caccgtgggc
     1201 ggcaagcttg cagatcgtgc gctgatgcct tcgctgatgg gcattctggt ggcgctcacg
     1261 gtggtgatgg cgattttcgc gcggaccagt cattcgcagg tggccgcggc gatcaccatt
     1321 ttcgtgtggg gcattgctgc ttttgcgacc gtgccgccgt tgcagatgcg cgtggtcgag
     1381 aaggccgctg ccgcgccgaa tctggcttcc acgctgaata tcggtgcgtt caacgtgggc
     1441 aatgcgggcg gcgcgtggct cggcggtctg gtgatcgatc acggtcattc gctcgatacg
     1501 ctgccttggg ttgcggcggc ggtgagcgtc gcggctttgt tgctgacgtg gttcgccgcg
     1561 cggatggatg cgccggcatc gaccgtcgcg cagggcgcgt gagtgtgcgt tttgtgcgga
     1621 tcccagtccg accccgccca tatgaaaaat tcgcacaagc tttgcgagaa tcgtcctccg
     1681 caatactgat aacagtgtga agataacttc gttgggcgcg gcgaggcgcg atgctatctt
     1741 gcttccacta accgcttgcc cgccgtccgg cggcgcgtct ggaggcaatc atgcattccc
     1801 cgcttgcgct ggttcgcgat cccacggctg acaccgatgc gccgtcgcgt gccactgaac
     1861 ccttcgacgc tcttgaacac ctggtcggcg tgaatctcgc ccgtttgcgc gccgagcgcc
     1921 aactgtcgct cgacgccttg gcccgcgcct ccggcgtctc gcgagcgatg ctcgcgcaaa
     1981 tcgaatcggc gcgcagcgtg ccgtcgatca aggtgctgtg caaggtggca gcggcgttga
     2041 aagtatcggt ggcggcgttt ctgcggcgtc atgcgaccaa tggcttcgag catttgccgg
     2101 cggagcgttc gtcgcggctc gtcagttcga atgggcgcta ttcggcccgg ccgctttatc
     2161 ccgacgccga gccgaccgcc gcggagtttc acgagttgcg tatcgcgccg ttgcataccg
     2221 aggccggcac gcgccgcgcg ccgggcacga cggtgaatct ggtagtgagc gaaggcacgc
     2281 tggaagtcag cgtgcacgat caacgccaat tgctggcaac cggcgacgcg atcgtcttcg
     2341 atgccgacca gccgcacagc ctgcgcaatc ccggcgacac ggaggcgcgc gcgtttcgtg
     2401 tgacgctgaa ggcggaaacg ccgccgcgct gggacgtgcc cgcaccgcac gatttgacgc
     2461 gccaggccgc gccggtttga cgg
//
LOCUS       DATASH010000892         5001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6085025,
            whole genome shotgun sequence.
ACCESSION   DATASH010000892 DATASH010000000
VERSION     DATASH010000892.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5001
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6085025"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1556)
                     /locus_tag="VHV99_23835"
     CDS             complement(<1..1556)
                     /locus_tag="VHV99_23835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HEX3638450.1"
                     /translation="MHVTHATPPNPSPLFLATKVFPPRLPAGLIDRPRLLSLAGKAEY
                     KRLTVIKAPAGFGKTSLALTWLDGLNAGGAFVAWLSLDTEDDEPARFFHHLAQALRNA
                     CASVGSSALSLTTEASLVPARSVVSTLINELVEVDEEVCLFVDDYHLISLFAIQDAMS
                     YFIANAPSNVHVVVCTRTDPPLPLANLRARNDLLEIDASALRFNFDETRSFVEHECPG
                     GLRAACVKSLFANTEGWAAALRISASVLSRDECKPDWDAGAPSGASRPFAAYLEDILK
                     RLRADMVGFMLRTSILDRLTASLCEAVTGIAPQQGMLETISARQLLLEPLDIEGHWFR
                     YHHLMGEYLRRRLETRHRDEVVDLHRRACQWYAKEEQWTDAVKHAIAAGDTQDAVSLM
                     GHCAMALVTKGDLLTLLGWQRQFPAHLMRGQVKVTLAIAWGMALAMRFDEALAILDEI
                     ELDAGAGSGEAQENIRWECQGIRSVVAGLQDDAPRALAIAQACLDRPSTDSWTTNVVS
                     NVVRFGHWKAG"
     gene            1933..2682
                     /locus_tag="VHV99_23840"
     CDS             1933..2682
                     /locus_tag="VHV99_23840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEX3638451.1"
                     /translation="MKVLVPVKRVVDANVRVRVKADGSAVDIANVKLSMNPFDEISVE
                     EAVRLKEAGVASEVVVVSCGVQGCQETLRTAMATGADRAILVQTDAELQPLAVAKLLK
                     ALVDKEQPQLVMLGKQAIDDDANQVGQMLAALLGWPQATFASKVRVVAGKATVTREID
                     GGLETIEVDLPAVVTTDLRLNTPRYATLPNIMKAKKKPLDVVTPDALGVDIAPRLTTL
                     KLVEPPKREAGIRVADAADLVAKLKNVAHVI"
     gene            2699..3646
                     /locus_tag="VHV99_23845"
     CDS             2699..3646
                     /locus_tag="VHV99_23845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX3638452.1"
                     /translation="MSILVIAEHDNQTLKAATLNTVTAAACLGDDIHVLVAGSQCAAV
                     AEQAASIAGVNRVRVADAPHYAHPLAENLATLVLTVAADYSHVLSPATTTAKNAMPRV
                     AALLDVAQISDIVAVEPADSFVHPIYAGNVLATVRSADHIKVITVRTTAFAAAQAVGG
                     TAAIEALAPAVEVPGSRFVSQQLTRSERPELTAAQRVISGGRGMGSCENFALLDDLAN
                     KLGAAVGASRAAVDAGFAPNDYQVGQTGKVIAPQLYIAVGISGAIQHLAGMKDSKVIV
                     AINKDEEAPIFQVADYWIVGDLFKVLPELSGELDKLQQN"
     gene            3661..4566
                     /locus_tag="VHV99_23850"
     CDS             3661..4566
                     /locus_tag="VHV99_23850"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEX3638453.1"
                     /translation="MSTPILHRWLAIAERAYQQAREYAKERIQGRLAGSKSGGAVAII
                     HHPDVRRMLMTMKAQIEAMRAFAYVMAADMDRAHRDPDVGERARRQVRVDLLIPVLKA
                     WCTELGVDIASMGVQVHGGMGYIEETGACQFLRDARIAPIYEGTTGIQAADLAGRKLA
                     SDQGAGMADLVAEMRQVAQELARSPDAQVASVGTALAAGIQALDDATVWMLHALATQP
                     DAALASSVDYLMLTGYVCGGWQMGRAALAASRKSAADEDPDFHHAKLATARFYADKIL
                     PKANALLETIRSGGSTGSSLPIEQF"
     gene            4640..>5001
                     /locus_tag="VHV99_23855"
     CDS             4640..>5001
                     /locus_tag="VHV99_23855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018435106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family protein"
                     /protein_id="HEX3638454.1"
                     /translation="MMKKLVYLALIPGAICSSAMAQSAGSLITSVGGAWLDFGPSSAT
                     SLQSSSPLGTFTSTGTGGQIHNTFTLELSNTYFVTDHIAFDLAAGVPPKLQLFAQGNA
                     RPFGAGGPSLNLGNLAPLA"
BASE COUNT          993 a         1489 c         1595 g          924 t
ORIGIN      
        1 cctgccttcc agtgcccgaa tcgcacgacg ttggagacga cattcgtggt ccagctgtct
       61 gtcgacggcc ggtcgaggca cgcctgcgcg atcgcaagcg cgcgcggtgc gtcatcctga
      121 agtccggcca ccaccgagcg gatcccttgg cattcccaac gaatattttc ttgtgcttcg
      181 cccgaacctg caccggcgtc aagctcgatc tcatcgagga ttgcaagcgc ttcgtcgaag
      241 cgcatcgcga gcgccattcc ccacgcgatc gcaagtgtca ccttgacctg cccgcgcatc
      301 aggtgcgccg ggaactgccg ttgccagccg agcagcgtga gcaggtcgcc cttcgtgacg
      361 agcgccatcg cgcagtgacc catcagggag acggcgtctt gcgtatcgcc cgcggcgatc
      421 gcgtgcttga ccgcgtcggt ccattgctcc tctttggcgt accactggca cgcgcgacga
      481 tgcaggtcca ccacttcatc gcgatggcgg gtttcgagcc gccggcgcag gtattcgccc
      541 atcaaatggt gatagcggaa ccagtgtcct tcgatgtcga gcggttcgag cagcaactgg
      601 cgcgcagaga tcgtctcaag catcccttgt tgcggcgcaa tgccggttac cgcttcgcat
      661 agcgacgccg tcaggcggtc gagtatcgac gtgcgcaaca tgaacccgac catatcggct
      721 cgcaaacgct tgagtatgtc ttcaaggtag gcggcgaacg gccgggaggc gccggacggc
      781 gcgcccgcgt cccaatccgg tttgcattcg tcccgcgata gcaccgaggc agaaattcgc
      841 agcgccgcag cccagccttc cgtgtttgcg aacaaggact tgacgcaggc agcgcgcagc
      901 ccgcctgggc attcgtgttc cacaaaactg cgggtttcgt cgaagttgaa acgaagtgcc
      961 gacgcgtcga tttccagcag gtcgttcctg gctctcaggt tggcaagcgg cagcggcggg
     1021 tcggtgcgcg tgcagaccac gacatgcacg ttggacggtg cgttcgcgat gaagtacgac
     1081 attgcatcct gaatggcgaa caggctgatc aggtggtagt cgtcgacaaa tagacagacc
     1141 tcctcgtcca cttcgacgag ttcgttgatc agcgtcgata cgactgagcg cgcaggcacg
     1201 agcgacgcct cggtggtcag gctgagcgcg gacgagccga cgctggcgca ggcgttgcgc
     1261 aatgcctgcg ccagatgatg aaagaagcgc gcgggttcgt cgtcctccgt gtcgagcgac
     1321 agccacgcga caaatgcgcc gcctgcattc agcccgtcga gccacgtgag cgcaagcgat
     1381 gtcttgccaa aaccggcagg cgccttgatg acggtaagcc gtttgtattc cgctttcccg
     1441 gcgagggaga ggagccgtgg ccgatcaatc aatccggcag gcaggcgcgg cggaaagacc
     1501 ttcgttgcga ggaagagagg cgacgggttc ggaggcgtcg cgtgcgtcac atgcatcagt
     1561 ggctccagat attcgggttg accctgactg ccatttcttg tctatgcgat cgcgctcccc
     1621 tgtaaggggg attttgcgtg gcgccgcgct cagacactat gggttccagt gaggagacgc
     1681 gcggttcgac aactgcaaaa cagcgccgga caacggtgcg gcctttgcct gaaccgatgt
     1741 tctgcttaag aaatgatacg cccaaccgcc ttgatgaagc agacatgtct gccgcgttca
     1801 atcgaatggc ggggtattgc gatgcacaaa ctcgggctcg cggtgcgtat cccgtacgaa
     1861 caagacgaaa cctcgatcga ccggttcgtg tggccggcgt ggcgtagtcc ttttatcgaa
     1921 acggagtagg caatgaaagt actggttcct gtgaaacgcg tcgtcgacgc gaatgtgcga
     1981 gtccgcgtga aagcggatgg cagcgccgtc gacatcgcta atgtgaagct gtcgatgaac
     2041 cccttcgacg aaatcagcgt ggaagaggcc gtgcgtttga aggaagccgg cgtggcgtcc
     2101 gaggtggtcg tcgtgtcctg cggggtgcag ggctgccagg aaacgctgcg caccgctatg
     2161 gcgacaggcg ctgaccgcgc catccttgtt cagaccgacg ccgagttgca gccgcttgcg
     2221 gttgcgaagc tgctcaaggc gctggtggac aaggagcagc cgcaactcgt gatgctcggc
     2281 aagcaggcta tcgacgacga cgcgaaccag gtcggccaga tgcttgcagc gctgctcggc
     2341 tggccgcagg cgaccttcgc atcgaaggtg agggtcgtag caggcaaggc gaccgtgacg
     2401 cgcgaaattg acggcggact tgagacgatc gaagtcgatc tgccagccgt cgtgaccacc
     2461 gatctccgcc tcaacacacc gcgctacgcg acgttgccca acatcatgaa ggcgaagaag
     2521 aaaccgctcg acgtggtgac acccgatgcg ctcggcgtcg acatcgcacc gcggctcacg
     2581 acgctcaagc tcgtggagcc gcccaaacgc gaggcgggta ttcgcgtggc cgacgctgcg
     2641 gatctcgtgg ccaagctgaa gaacgtcgca cacgtcatct gaaaaggaag ggggagacat
     2701 gtccatactt gttattgcag aacacgacaa tcaaacgctg aaggccgcga cgttgaatac
     2761 tgtcaccgcc gccgcgtgcc ttggcgacga cattcacgtt cttgtcgccg gatcgcaatg
     2821 cgcggcagtg gccgaacaag ccgcgagcat cgccggcgtg aaccgcgtgc gagtcgccga
     2881 tgcgccgcac tatgcgcatc cgttggccga gaacctggcc acgctggtct tgacggtcgc
     2941 agctgactac agccacgtgc tgtcgcccgc cacgaccacc gcgaagaacg cgatgccgcg
     3001 cgtggctgcg ctgctcgacg tcgcgcagat ctcggatatt gttgccgtcg agcccgccga
     3061 tagtttcgtg catcccattt acgcgggcaa cgtgctggcg acggtgcgaa gcgcggacca
     3121 catcaaggtc atcacagtgc gcacgacagc ctttgcggcg gcgcaggccg ttggcggcac
     3181 ggcggcgatc gaagcactcg cgccagccgt cgaagtccct ggctcacgtt tcgtgagcca
     3241 gcaactgacg cgctccgagc gccctgagtt gaccgcggcg cagcgagtga tctcgggtgg
     3301 ccgaggtatg ggttcctgcg aaaacttcgc actgctggat gatctcgcca acaaacttgg
     3361 cgcggcagtc ggcgcgtcgc gtgctgccgt ggatgcaggc ttcgcgccga acgattatca
     3421 ggtgggacag accggcaagg tcatcgctcc gcagctctat atcgcggtcg gcatctccgg
     3481 ggcgatccag catctggccg gcatgaagga ctcgaaggtg atcgtcgcga tcaacaagga
     3541 cgaggaggcg ccgatctttc aggtcgccga ttactggatc gtcggtgatc tcttcaaggt
     3601 attgccggag ttgtccggtg aactggacaa gctgcaacaa aactaacgcc ggagacgacg
     3661 atgagcacac ctatattgca ccgctggctc gcgattgccg aacgcgctta tcagcaagcc
     3721 cgcgaatatg cgaaggaacg catacagggg cggctcgcgg ggagcaaatc gggtggcgca
     3781 gtggcgatca ttcatcaccc ggatgtgcgg cgcatgttga tgaccatgaa ggcccagatc
     3841 gaagcgatgc gcgctttcgc atatgtcatg gccgctgaca tggaccgcgc gcatcgggac
     3901 cctgacgtcg gcgagcgcgc acgtcgccag gtgcgcgtcg atcttctgat tcccgtgttg
     3961 aaggcatggt gtacggagtt aggcgtcgat attgcgtcaa tgggcgtgca ggtgcacggc
     4021 gggatgggct acatcgaaga gactggcgca tgccagttcc tgcgcgacgc acgaattgct
     4081 ccgatctacg aaggcacgac cggcatccag gctgccgacc tcgcaggccg caagctcgcg
     4141 tcagatcagg gcgccgggat ggcagacctt gttgcggaga tgcgccaggt tgcgcaggag
     4201 cttgcgcgca gcccggatgc acaagtggcg tccgttggca cggcgctcgc ggctggtatc
     4261 caggcgctcg acgacgccac cgtctggatg ctgcacgcat tggcaacgca gcccgacgca
     4321 gcgcttgcca gttccgtcga ctatctgatg ctcaccggct acgtgtgcgg aggctggcag
     4381 atggggcgcg cggcgctcgc tgcgtcaaga aaaagcgcgg cggatgagga cccggacttc
     4441 catcacgcga agcttgccac tgcgcgcttc tacgccgaca aaattttgcc caaagcgaat
     4501 gcacttctgg agacgatccg cagtggcggg tcgactgggt cgagtctgcc aatcgagcag
     4561 ttctgaacag ttctgagcgg cgcaacaaaa tattgatgca aaaaatattg atatgtaatg
     4621 cgaaataaat aggggaaaaa tgatgaaaaa actcgtatac cttgcgttaa taccgggtgc
     4681 gatatgttcc agtgcgatgg cgcagagcgc gggcagtctt atcaccagcg tgggtggagc
     4741 gtggctcgat tttggtccgt cttccgcaac gtcgttgcag agcagttcgc ctttgggcac
     4801 atttacttca acgggcacgg gtggtcagat acataacacg ttcaccctgg agctatcgaa
     4861 cacttatttc gtcactgacc acattgcttt tgacctggca gcgggcgtac cgcccaagct
     4921 ccagctcttt gcgcagggca acgccagacc gttcggtgct ggcgggccat cgctcaacct
     4981 ggggaatctg gcgccgctcg c
//
LOCUS       DATASH010000893         9056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5692602,
            whole genome shotgun sequence.
ACCESSION   DATASH010000893 DATASH010000000
VERSION     DATASH010000893.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 9056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9056
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5692602"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2668
                     /locus_tag="VHV99_23860"
     CDS             <1..2668
                     /locus_tag="VHV99_23860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018425366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX3638455.1"
                     /translation="LQAKGLAPLDVVNAVNAQNLILPGGTAKIGTKEYNVQMNGSTDT
                     VAALNNLPIKTVGGGVVYVHDVAQVRDGYAPQTNIVRSDGKRAALLTIEKSGSTSTLT
                     IIQQVKAMLPHIAAGLPSALHITALADQSVFVKSAVLGVAREALIAACLTALMILLFL
                     GSWRATLIIAVSIPLAVLTSLIALAALGETINIMTLGGLALAVGILVDDATVAIENIT
                     HHLENGEPLHDAILNGSGEIAVPTFVSTLSICIVFVPMFLLSGVARYLFVPLAEAVVF
                     AMIASYFFSRTLIPTLALYLMRPRGNAPVTGNGPIAQLVRFQAGFEHRFELLRERYRG
                     VLSAGIARPRRFVAIFLLLCIGSLALVPFSGRDFFPAVDTGEIRLHLRAPTGTRIEDT
                     ASLTDQVEARVRSMIPKQELSAMLDNIGVPVSGINLTYDSSDPIGPEDADVMITLAAK
                     HKPTADYVATLRTTLAKDFPGVSFAFLPADIVSQILNFGLPAPIDVQIVGNKLNANRV
                     VANRLLTQLRGVRGLVDARIQQPGDEPAININVDRTRAIQAGLQQRDVAQNLLIALSG
                     SSQTTPNFWLDPKNGVSYPLMAMTPQYDIDSLQALANIPVAPSGTANSAASTAAAFAT
                     GTGASPQNLLGALGTLSRGTQQAVVSHYNVQPVLDIFASAQGRDLGGVATDVNRLVDQ
                     IRPQLPPGASIVVRGQVQAMHDSFAGLASGMVFAIALVYLLMVINFQSWLDPFITISG
                     LPGSLAGISWMLFSTGTRLSVPALTGTILCIGIATANSILVINTARELHHGGKPPLQA
                     ALEAGFSRFRPVLMTALAMLIGMLPMALGLGDGGEQNAPLGRAVIGGLALGTVSTLLF
                     VPVVYGMVHAWRDKRRAARQSTQEADLAARTH"
     gene            2688..3968
                     /locus_tag="VHV99_23865"
     CDS             2688..3968
                     /locus_tag="VHV99_23865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011545697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX3638456.1"
                     /translation="MNDSSLPPELDDPAGQSANRADRNVENAAENAAKHAAQAPAAAA
                     HPAPRRRLWPLALTGLAAALLLAGILPRVSESAALTQQTQAQSALSVSVVMPRPAPAS
                     NELLLPGAVTPFAEASIYARTSGYLAHWTSDIGAQVKSGQTLATIEAPELDAQLRQAR
                     ADEATAQANYDLAKTTALRWQAMLKTQSVAQQDADTKTSDMEARSAMLASAQANVAHL
                     AELVSYEKVTAPFDGVITARHVDVGALVTAGGTPGTAGMSGELFHIQQTGVLRVFVDV
                     PQNDAPYVTPDTAVYLTAQQYPGRHIAGRVARSTGSIDPASRTLRVEVDVDNKDGALM
                     PGAYVQVHLALQSSTPALDLPVSALLFRPDGVTVAVVDGNGDVVLKTVHVGRDFGTHV
                     EITTGLAATDRVIDNPGDSLSTGQVVQIAPATHN"
     gene            3987..5522
                     /locus_tag="VHV99_23870"
     CDS             3987..5522
                     /locus_tag="VHV99_23870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011880459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEX3638457.1"
                     /translation="MYAMPFTFTCPLLRRSLTVALLGALAACSTLPAYQRPTAAVPAQ
                     FAGATPGLAQAAPADAAPRGPWWTIFGDPVLDQLEARIDVSNQTVRKAVTQVQQARSM
                     VDYQRGGFFPTITAGTSQERFRTSQNIEYRAVAGKTIPDYSAGVSASWEPDLFGRVRD
                     SVANAQATADASAADLEAVRLSMSSDLAVDYFALRSLDTQKKLLDDSVHAYAEALNLL
                     QQQLKNGAIDASAVAQATTQLEATRTQDTDIDVTRAQMQHAIATLVGEPATTFALPAN
                     GSAVAPPAIPVGLPSQLLERRPDIAAAERRVAAANAQIGMAHAAFFPDLMLSASAGLE
                     SSFFAPWLSAPSLFWSLGSQLVGTLFDGGQRSATLHGATAQYDGTVADYRQSVLVAFQ
                     QVEDNLSALNSLADEAGSQQLATSAADLSLRLTTNRYNAGAVSYLDVVTAQTIALTNE
                     RTADQIAARRLESSVLLMKALGGTWYDVGTSATAADVAAAAVAAKPNGGDVAAVAAKP
                     NGG"
     gene            complement(5829..7361)
                     /locus_tag="VHV99_23875"
     CDS             complement(5829..7361)
                     /locus_tag="VHV99_23875"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HEX3638458.1"
                     /translation="MRGRSLAATLAIAFGVTTLAVFVLVGSYLYLALERQIKTQDNLD
                     IVLAARHARRLAQELETPKGIREHSDRLTSIVLGNEAMSMEVFGPDGNEVIEHNAAGA
                     FAAPRTANEAGSASATGVTSATSVTGGTSPTSGALIITALPPLERVPASARITEADIG
                     EWMGRNGAPIRGVLTDVRLRNGDTASVLVARNMSDRWLLLDRYRDELKLAGTAGLLLA
                     IVLSYLLIRAALRPLRDIAASASLVTVNRLHTRIEAARVPSELKALVSALNAMFERVE
                     LGFQRLSRFTADLAHDMRTPLSNMRGAAEVALARPRSVDEYETLLASNLEECDRLSRM
                     IESVLFLARAEHPQFVKRMREFGAALELKHIADYFEGIAEEAHVTMRVSGAASLTADL
                     ELFRRAVSNLLANAIRYTPRGGEIVLDAAASAAAVRITVSNPGQPIAAEHLERIFDRF
                     YRIDPSRSTLPSAGLSQASTGSTGLGLAIVRTIMELHGGTVRAESDEHGTRFILTFLR
                     TA"
     gene            complement(7361..8038)
                     /locus_tag="VHV99_23880"
     CDS             complement(7361..8038)
                     /locus_tag="VHV99_23880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006411957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal response regulator transcription
                     factor"
                     /protein_id="HEX3638459.1"
                     /translation="MRILVIEDELKTAAYLRKGLEESGYAVDVANDGPQGLILAQEEE
                     YDVIVLDVMLPGMDGWTVVKTLRNTRTTPVLFLTARDDVDDRVRGLELGADDYLVKPF
                     AFVELLARVRTLARRGPPRESELIKVGDLEMDVNRRRVKRGGLRIDLTPREFSLLQLL
                     ARRHGEVLSRTQIASYVWDMNFDSDTNVVEVAIRRLRTKIDDNFPVKLIHTVRGVGYV
                     LEVKDVG"
     gene            complement(8097..>9056)
                     /locus_tag="VHV99_23885"
     CDS             complement(8097..>9056)
                     /locus_tag="VHV99_23885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3638460.1"
                     /translation="LALRLALHTLWPLGVLVPVTIVLVLLVVARGLAPIGGLSRALAA
                     HSIDSPEPLRLDGAVPVEIKPLVEALNDLLQRLGTASQAQRTFIADAAHELRSPLAAL
                     KLQLQAASRDGSLKGEGQTLERLEGRVNRIIHLVQQLLTLAREDAHPATSMVPTSLRR
                     IGEQVVGDFSLLAEAGEIDLGLECEAARGASDAYTVLAEPHGMSVLLGNLVNNAIRHT
                     PRGGRVDVILRRAGDRLGFDVVDSGSGIPAAEIERVFDRFYRGEDARGEGSGLGLAIV
                     SRIAERHRLALSLRNNADRPGLRVSVFGLAEHEIGIRSDITGS"
BASE COUNT         1546 a         3064 c         2915 g         1531 t
ORIGIN      
        1 attgcaggca aaaggtcttg cgccgctcga cgtggtcaac gccgtgaacg cgcagaatct
       61 gatcctgccg ggcggcaccg caaagatcgg cacgaaggaa tacaacgtgc agatgaacgg
      121 cagcacggac acggtcgccg cattgaacaa tctgccgatc aagacggtag gcggcggcgt
      181 ggtgtatgtg cacgatgtgg ctcaggtgcg cgacggttac gcgccgcaaa ccaacatcgt
      241 gcgcagcgac ggcaagcgcg ccgcactcct gaccatcgag aaatccggca gcacgtcgac
      301 gctgacgatc atccagcagg tcaaggcgat gctgccgcat atcgcggccg gtttgccgag
      361 cgcgctgcac atcacggcgc tagccgacca gtcggtgttc gtcaaatccg cggtgctcgg
      421 cgtcgcccgc gaagcgctga tcgccgcctg cctgaccgcg ttgatgatcc tgctgttcct
      481 cggcagctgg cgcgccacgc tgatcatcgc cgtgtcgatt ccgctcgcgg tgctgacctc
      541 gctgatcgcg ctggccgcgc tcggcgagac catcaacatc atgacgctcg gcggccttgc
      601 gctggccgtg ggcattctcg tcgacgatgc caccgtcgcg atcgaaaaca tcacgcacca
      661 cctggagaat ggcgagccgc tgcatgacgc gattctgaac ggttcgggag aaatcgccgt
      721 gccgaccttc gtgtcgacgc tgtcgatctg catcgtgttc gtgccgatgt tcctgctttc
      781 cggtgtcgcg cgctatctgt tcgtgccgct cgccgaagcg gtggtgttcg cgatgatcgc
      841 ctcgtatttc ttctcgcgca cgttgattcc gacgctcgcg ttgtatctga tgcggccgcg
      901 cggcaatgcg ccggtgacgg gcaacggacc gatcgcgcaa ctcgtgcgct ttcaggccgg
      961 cttcgagcat cgttttgaat tactgcgcga acgctaccgc ggcgtgctca gcgcgggtat
     1021 cgcgcgcccg cgccgcttcg tcgcgatctt cctgctgctg tgcatcggct cgctcgcgct
     1081 cgtgccgttt tccggacggg acttcttccc cgctgtcgat accggtgaga tccggctgca
     1141 tttgcgcgcc cccaccggca cgcggatcga agataccgca agcctcaccg atcaggtcga
     1201 agcgcgtgtg cgtagcatga ttccgaagca ggagttgtcc gcgatgctcg acaacatcgg
     1261 cgtgccggtg agcggcatca acctgaccta cgattcatcg gacccgatcg gccccgaaga
     1321 cgccgacgtg atgatcacgc tcgcggcaaa acacaagccg accgcggact atgtcgcgac
     1381 gctacgcacc acactcgcca aggacttccc gggcgtgagc ttcgccttcc tgccggccga
     1441 catcgtcagc cagattctga atttcggcct gcccgcaccg atcgatgtcc agatcgtcgg
     1501 caataaactc aatgccaacc gcgtggtggc gaaccgcttg ctcacgcaac tgcgcggcgt
     1561 gcgtggcctt gtcgacgcgc ggatccagca gccgggcgac gagccggcga tcaacatcaa
     1621 cgtggaccgc acgcgggcaa tccaggcggg cttgcagcaa cgcgacgtgg cgcagaatct
     1681 gctgatcgcg ctgtccggca gctcgcagac cacgccgaat ttctggctcg atccgaagaa
     1741 cggcgtcagc tatccgctga tggcgatgac gccgcagtac gacatcgact cgctgcaggc
     1801 actggccaac attcccgtgg cgccaagcgg caccgcgaat tccgctgcaa gtaccgcggc
     1861 cgcttttgcc actggcaccg gcgcgagccc gcagaacctg ctcggcgcgc ttggcacgct
     1921 gtcgcgcggc actcagcaag ccgtcgtctc gcactacaac gtgcaacccg tgctcgacat
     1981 tttcgcgtcg gcacaaggcc gcgacctcgg cggcgtcgcg accgacgtga accgtctggt
     2041 cgaccagatc cggccgcaat tgccgccggg cgcctcgatc gtcgtacgcg gccaggtgca
     2101 ggcaatgcac gactcgttcg ctggcctcgc aagcggcatg gtgttcgcga tcgcgctcgt
     2161 ctatctgctg atggtcatca atttccagtc gtggctcgat ccgttcatca cgatcagcgg
     2221 cttgccgggc tcgctcgcgg gcatctcgtg gatgctgttc agcaccggta cccggctgag
     2281 cgtcccggcg ctcaccggca cgattctgtg tatcggcatc gccacggcca acagcattct
     2341 cgtgatcaac accgcacgcg aactgcacca cggcggcaag ccgcccttgc aggcggcgct
     2401 ggaagccggc ttcagccgct tccgccccgt cttgatgacc gcgctggcga tgctgatcgg
     2461 catgctgccg atggcgctcg gcctgggcga cggcggcgaa cagaacgcgc cgctcggccg
     2521 cgcggtgatc ggcggcctcg cactcggcac ggtctccaca ctgctgttcg tgccggtcgt
     2581 ctacgggatg gtgcatgcgt ggcgggacaa acgacgcgcc gcccgccaga gcacccagga
     2641 agccgacctt gccgcccgca cccattgatt ccgacgagag cctccctatg aacgattcat
     2701 ctcttccgcc cgaactcgac gatcccgcag ggcagtccgc gaaccgcgct gaccgtaacg
     2761 tggaaaatgc cgctgaaaat gccgcgaaac acgcggcgca agcgcccgcg gcggccgcgc
     2821 atcctgcacc gcgccggcgc ctgtggccac tcgccctgac cggccttgcg gccgccttgc
     2881 tgctcgccgg catcctgccg cgtgtctccg agagcgccgc cctgacgcaa cagacccagg
     2941 cccaaagcgc gctcagcgta tcggttgtca tgccgcgccc cgcgccggcc tcgaacgagt
     3001 tgctgttgcc cggcgcggtc acgccgttcg cggaggcgtc gatctatgcg cgcacgagcg
     3061 gctatctggc gcactggacg agcgacatcg gcgcccaggt caaaagcggc cagacgctcg
     3121 cgacgatcga agcccccgag ctggatgcgc aactgcgcca ggcccgcgcc gacgaagcca
     3181 ccgcgcaagc caactacgac ctcgcaaaga ccaccgcgct gcgctggcag gcgatgctga
     3241 aaacgcagtc ggtcgcgcag caggacgcgg acacgaaaac gagcgacatg gaagcgcgca
     3301 gcgcgatgct ggcgtccgcg caggcgaacg tcgcgcatct ggccgaactg gtctcgtacg
     3361 aaaaagtcac cgcgccgttc gacggcgtga tcaccgcgcg ccacgtcgat gtcggcgcgc
     3421 tggtgacggc gggcggcacg cccggtaccg ccggcatgtc tggcgagctg tttcatatcc
     3481 agcagaccgg cgtgctgcgg gtcttcgtcg acgtaccgca gaacgacgcg ccgtatgtca
     3541 cgccggacac cgccgtctat ctgactgccc agcagtatcc cggccgtcac attgccgggc
     3601 gggtggcgcg cagcaccggc tcgatcgatc cggcgagccg cacgttgcgc gtcgaggtgg
     3661 acgtcgacaa caaggacggc gcgttgatgc cgggcgccta tgtgcaggtg catctggcgt
     3721 tgcaatcgtc gacgcccgcg ctcgatttgc cggtcagcgc gctgctgttt cgcccggatg
     3781 gcgtgacggt cgcggtggtc gacggcaacg gcgacgtcgt actgaagacc gtgcacgtcg
     3841 gccgcgattt cggcacccat gtggaaatca ccacggggct ggcggccacg gatcgcgtga
     3901 tcgacaaccc gggcgattcg ctgagtacgg gacaagtcgt ccagatcgca ccggccacgc
     3961 ataactgagc gaccgtcatc gtaaggatgt atgccatgcc ttttaccttt acttgcccgt
     4021 tgctgcggcg ctcgctgacc gtggcgctgc tcggcgcgct tgccgcgtgc tcgacgctcc
     4081 ccgcttacca gcgccccacc gccgcagtgc ccgcgcaatt cgcgggcgcc acgcccggct
     4141 tggcgcaggc ggcgcccgcc gacgccgcgc cgcgcgggcc ctggtggacg attttcggcg
     4201 atcccgtgct cgaccagctc gaagcgcgca tcgacgtctc gaaccagacc gtccgcaagg
     4261 ccgtcacgca agttcagcag gcgcgctcga tggtcgacta tcagcggggg ggatttttcc
     4321 cgaccattac cgccggcacc tcccaggagc gcttccggac ctcgcagaac atcgagtacc
     4381 gcgcggtcgc gggcaagacc atccccgact attcggcggg cgtgtcggcg agctgggagc
     4441 ccgatctgtt cggccgcgtg cgcgacagcg tcgcgaacgc gcaagccact gccgacgcca
     4501 gcgcggcgga cctcgaagcg gtgcgtctgt cgatgagctc ggatctcgcc gtcgactatt
     4561 tcgcgctgcg ctcgctggat acgcagaaaa agctgctcga cgacagcgtg catgcgtacg
     4621 ccgaagcatt gaatctgctc cagcagcagc tgaagaacgg tgcgatcgac gcctccgcgg
     4681 tcgctcaggc caccacgcag ctcgaagcga cacgcactca ggacaccgac atcgacgtga
     4741 cccgcgcgca gatgcagcac gcgatcgcga ccctggtcgg cgaaccggct acgacctttg
     4801 ccctgccggc gaacggctcg gcagtggcgc cgccggccat tccggtcggc ttgccctcgc
     4861 agttgctcga gcggcggccc gatatcgccg ctgccgaacg gcgcgtggcg gcggcgaatg
     4921 cgcagatcgg catggcgcat gcggcgttct tccccgatct gatgttgtcg gcgagcgcgg
     4981 ggctggaaag cagcttcttc gcgccgtggc tgagcgcgcc gagcctgttc tggtcgctcg
     5041 ggtcgcaact cgtcggcacg ctgttcgacg gcggccagcg cagcgccacg ctgcatggcg
     5101 cgaccgcgca atatgacggc acggtcgccg attaccgcca gtcggtgctg gtggcgttcc
     5161 agcaggtcga ggacaacctc tcggcgctga attcgctcgc ggacgaagcc ggcagccagc
     5221 agctcgcaac cagcgccgcc gatctgtcgc tgcgcctcac gaccaatcgt tacaacgcgg
     5281 gcgcggtcag ctatctcgac gtggtgaccg cgcagacgat cgcgttgacg aacgagcgca
     5341 ccgcggatca gattgcggcg cgccggctgg agtcgagcgt gttgttgatg aaggcattgg
     5401 gcgggacgtg gtatgacgtt ggcacgagcg caacggcggc tgatgtggcg gctgcggcgg
     5461 ttgccgcgaa gcccaacggt ggtgatgtgg ctgcggttgc cgcgaagccc aacggcggtt
     5521 gatgtggctg cggcggttgc cgcgaagccc aaccgcggtt gatgtggcag ttgctgcggt
     5581 tgccgcgaag cctaacggcg gctgatgtgg cggcggtggt tgccgcgaag cccacggcgg
     5641 ctgatgtggc ggctgcggtt gccgtgaagc ccaacggtgg ttgatgtggc ggctgcggtg
     5701 gttgccgcga agcccggcgg cggttgccgc gcagcccggc ggcgctgaag ccggaattga
     5761 tccggctgca gccaagcgtg cgatgtgacc cgtggtgccg tccgaagtac ggccagaagt
     5821 gcggcctgtt acgcagtccg caggaacgtg agaatgaagc gcgtgccgtg ctcgtcgctt
     5881 tccgcgcgca cggttccgcc atgcaattcc atgatggtgc gcacgatggc aaggccgagt
     5941 cccgtcgatc ccgtggaggc ttgcgacaaa cccgcggaag gcaacgtgct gcgcgacgga
     6001 tcgatccggt agaaacggtc gaagatgcgt tccagatgct cggccgcgat cggttgcccc
     6061 gggttcgaca cggtgatgcg cacagcggcg gcggatgcgg cggcgtcgag cacgatctcg
     6121 ccgccgcgcg gcgtatagcg gatcgcgttg gcgagcaggt tgctgacggc gcggcggaac
     6181 agttcgaggt ccgcggtcag ggacgccgcg ccgctcacgc gcatcgtgac atgcgcttct
     6241 tcggcaatgc cttcgaagta gtcggcgata tgcttcaact ccagggcggc gccgaactcg
     6301 cgcatgcgtt tgacgaactg aggatgttca gcgcgagcca gaaacaggac gctttcgatc
     6361 atgcgcgaca ggcgatcgca ctcttcgagg ttcgacgcga gaagcgtttc gtattcgtcg
     6421 acggagcgcg gccgggcgag cgcgacttcc gccgcgccac gcatattgct caacggggta
     6481 cgcatatcgt gagcgaggtc ggcggtaaaa cgcgacaagc gctgaaagcc cagttcgacg
     6541 cgctcgaaca tcgcattcaa tgcgctcacc agcgccttga gttcgctggg cacgcgcgcg
     6601 gcctcgatgc gcgtatgcaa ccggttgacc gtcacgaggc tcgcgctcgc cgcgatatcg
     6661 cgcagcggcc gcagcgcggc gcggatcagc agataactca gcacgattgc cagcaggaga
     6721 cccgccgtac cggccagttt gagttcgtca cggtaacggt ccagcaaaag ccagcggtcg
     6781 ctcatgttgc gcgccaccag tacgctggcc gtgtcgccgt tgcgtaaccg tacgtctgtc
     6841 aggacgccgc ggatcggcgc gccattgcgg cccatccact cgccgatatc cgcttcggta
     6901 atgcgtgccg aagccggaac gcgttcgagc gggggcagcg cggtgatgat cagggcaccg
     6961 cttgtcgggc ttgtgccgcc ggtcacgctt gttgcgcttg tcacgcccgt tgcgcttgcc
     7021 gagccggcct cattggccgt acgaggcgcc gcgaaggccc ctgcggcgtt gtgttcgatc
     7081 acctcgttgc cgtccgggcc aaagacctcc atcgacattg cttcattgcc gagcacgatg
     7141 ctggtgagac gatcgctgtg ctcacgaatg cctttcgggg tttccagctc ctgagcgaga
     7201 cggcgcgcat ggcgagcggc aagcacgata tcgaggttgt cctgtgtctt gatctgccgt
     7261 tcgagcgcga ggtacagata actgccgacc agcacgaaca ccgccagcgt ggtgacgccg
     7321 aatgcaatgg ccagcgtggc ggccagcgag cggccgcgca tcaccccacg tccttcactt
     7381 cgagcacata gccgacgccg cgcacggtat gaatcagttt gacggggaag ttgtcgtcga
     7441 tcttggtgcg cagccggcga atcgccactt caacgacgtt ggtgtcgcta tcgaagttca
     7501 tgtcccacac ataggatgcg atctgcgtgc ggctcagcac ctcgccgtgg cggcgcgcga
     7561 gcagttgcag cagcgagaac tcgcgtggcg tgagatcgat acggagaccg ccgcgcttga
     7621 cgcgccggcg attgacgtcc atttccagat cgccgacctt gatcagttcg ctttcgcgcg
     7681 gcggcccgcg acgcgccagc gttcgcacgc gcgccagcaa ttccacgaac gcaaagggct
     7741 tgaccagata gtcgtccgcg ccgagttcca gaccgcggac gcgatcgtcg acgtcgtcgc
     7801 gggcggtgag gaacagcaca ggcgtggtcc gcgtgttgcg cagcgtcttg acgacggtcc
     7861 agccgtccat gccgggcagc atgacgtcga gcacgatgac gtcgtactct tcttcctgtg
     7921 cgaggatcaa accttgcggg ccgtcattgg cgacgtcgac cgcgtagccg gattcctcca
     7981 gaccttttct cagataggcc gctgttttca gctcgtcctc gatcaccagg attcgcattt
     8041 gacgctccgc gttgcattga tctctaaatc ataccggcaa gcgcggtgca tcgtgattac
     8101 gatccggtaa tgtcagacct gatcccgatc tcatgctcag ccaggccaaa aaccgaaacc
     8161 cgcaggccag gccggtcagc attgttgcgc agcgaaagcg caagtcgatg ccgctcggca
     8221 atccgcgaca caatcgcaag cccaagcccg ctcccctcgc cccgcgcgtc ttcgccgcga
     8281 taaaaccggt cgaaaacccg ttcgatctcg gccgccggaa tcccggaccc gctatccacc
     8341 acgtcgaatc cgaggcgatc gccggcacgc cgcaggatca cgtcgacccg cccgccgcgc
     8401 ggagtatggc gaatcgcgtt gttgaccagg ttgcccaaca atacactcat cccgtgcggt
     8461 tcggcgagca ccgtgtacgc atccgaagcg ccgcgcgccg cttcgcactc gagtccgaga
     8521 tcgatctctc ccgcctccgc gagcaaggaa aaatcgccca ccacctgttc gccgatccgc
     8581 cgcagactcg tcggcaccat cgacgtcgcc ggatgcgcgt cttctcgcgc cagcgtcaac
     8641 aactgctgca ccaggtgaat gatccgattc acgcgccctt cgagacgctc cagcgtctgc
     8701 ccttcgccct tcaacgaacc gtcgcgcgag gccgcctgca attgcagctt cagcgccgcg
     8761 agcggtgaac gcaactcgtg cgcggcgtcg gcaatgaacg tgcgctgcgc ttgcgaggcc
     8821 gtgccgaggc gctgcagcag atcgttgagc gcctccacca gcggcttgat ttcgaccggc
     8881 acggcgccgt ccagccgcaa cggctccggc gaatcgatcg agtgtgccgc gagcgcgcga
     8941 gacaggccgc cgatcggcgc caggccgcgc gccaccacca gcagcaccag cacgatcgtc
     9001 accggcacca gcacgccgag cggccatagc gtatgcagcg ccagccgcag tgcgag
//
LOCUS       DATASH010000894         4518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4504631,
            whole genome shotgun sequence.
ACCESSION   DATASH010000894 DATASH010000000
VERSION     DATASH010000894.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4518
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4504631"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..2334
                     /gene="tkt"
                     /locus_tag="VHV99_23890"
     CDS             226..2334
                     /gene="tkt"
                     /locus_tag="VHV99_23890"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX3638461.1"
                     /translation="MSTTEAQPAASDDSPDSLAASTPALFVAATQATQATQATQAEQA
                     TRPPQLPLADAIRFLSIDAILQAGEGHQGVPLGMAEIATALFTRHLKFNPADPSWPDR
                     DRFVLSNGHGSLLLYSLLYLTGYAAIGLDQLKTFRELGSHCAGHPEYEPAHGIEATTG
                     PLGQGIANAFGMAIAEAYLAAKFGSELVDHYTYAFVGDGCLQEGIGQEMISLAGHLRL
                     GKLILCWDDNQITDDGSTALSISEDVGARFRVAGWHVIEVDGHDLEAVSAALTIARKD
                     PRPSLLACKTIIGRGIARLQGQRGGHSGKLFQADADAAREQLGWPHAPFDLPAQVRDA
                     WVDAGRRSSDEYIAWHARLAALPDAQCAEFERVLAGGLPQGWRDVLDAYRKRAVERDE
                     PQPGITVSGEISDLFAPLLPERMIGCADLEAPTGHKRQLHAFTADDRSGAYVHCGVRE
                     HLMGAMANGMAAHGGIVATSVTYLAFSDYQRPAMRMAALMGLPVHFVFSHDSIGVGKN
                     GPTHQPVEILASLRAMPNMRVLRPADAVEAAECWALALEHRHGPSTLVFARQALPPVR
                     REHSAEPLSQRGAYVLAEAQGGPRAVTLLATGSEVALALQARSQLQDEGIATAVVSMP
                     CWEIFDEQDADYRATVLGVNTVRVGVEAALRFGWDRYLGERGGFVGMTGFGASASAET
                     LYEHFGITAQHVVAEAKRHL"
     gene            2363..3331
                     /locus_tag="VHV99_23895"
     CDS             2363..3331
                     /locus_tag="VHV99_23895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEX3638462.1"
                     /translation="MQKIVFLDRATLAPQIVLREPRFDHQLIEHDRTLPEQVLTRLAG
                     ASIAITNKVPLTKEVLAQLPDLKLVAVAATGTDCVDKAACQQLGIAVSNIRGYAINTV
                     PEHTFALILALRRNLIAYRDDVLAGEWQKSGQFCFFTHPIHDLAGARLGIIGEGVLGQ
                     RVAEIGKAFGMQPMFAAHKGRDGLGPLYTPWDEVLATSDIITVHSPLTPHTRNMLAMP
                     EFRAMKKKPLIINTARGGLVDEAALVQALDEGLISGAGFDVTAGGEPPPPESPLLRAA
                     GRPNVILTPHVAWASDEAQQSLADQLMDNIENFVDGAPTHLVQGAY"
     gene            3831..4484
                     /locus_tag="VHV99_23900"
     CDS             3831..4484
                     /locus_tag="VHV99_23900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638463.1"
                     /translation="MLVVEVVFGVFLLSHKSHRATTDPDVTTSNAVELAATSPQSGDT
                     HVTAGSVISAAPLSGDTATAATGDTAAATGAKQSTSGAVVTASDSPAKDAVQVPRLNT
                     RARESVAIHAYAGPKTGALPQTEIGRDSLHRHGSNPVGAAITDALVKESAKLDPALPP
                     PTQAIQPLQPIQAAQPALDDSYHRGSNPVAAAMTEQLVRESAKLDPGLPPPNQASMK"
BASE COUNT          853 a         1442 c         1396 g          827 t
ORIGIN      
        1 atcctgacga agggatgaag ccgattctcg gctacatcgc cgccttgatg gtcgggctga
       61 tcgtcgtggc cgcggtgccg tggatttcca tcggcttcct gcattgatgc gctgacggat
      121 gaggctgcgc cgcgcagcct ctgttgcgac attcgttcac tccattcgat tcgttttgat
      181 tgccgccgcc tcgcgtgtcg aggcggcgta gaggagcctt ttgccatgtc caccactgaa
      241 gctcaaccgg ctgcgtcgga tgattcgcct gattcgcttg cagcgagtac gccggcgctg
      301 ttcgttgcgg caacacaagc aacacaagca acccaagcaa cccaggcaga gcaagcaacg
      361 cgtccgccgc agttaccgct cgccgacgcg atccgcttcc tctccatcga tgcgatcttg
      421 caggccggcg agggccatca aggcgtgccg ctcggcatgg ccgaaatcgc caccgcgttg
      481 ttcacccgtc acctgaaatt caatcccgcc gatccgagct ggccggatcg cgaccgcttc
      541 gtgctgtcca acggccacgg ttcgctgctg ctgtattcgc tgctgtacct gaccggttac
      601 gcggcgatcg gcctcgacca actgaagacc tttcgcgaac tcggctcgca ttgcgcggga
      661 catcccgagt acgaaccggc gcatggtatc gaagcaacca ccggtccgct cgggcagggc
      721 attgccaacg cgttcggcat ggccatcgcc gaagcgtatc tggcggcgaa attcggcagc
      781 gaactcgtcg atcactacac gtatgcgttt gtcggcgacg gttgtttgca agaaggcatc
      841 ggtcaggaaa tgatttcgct ggcggggcac ttgcggctcg gcaaactgat tctctgctgg
      901 gacgacaacc agatcaccga cgacggcagc accgcgctat cgatcagcga agacgtcggc
      961 gcgcgatttc gcgtggccgg ctggcatgtg atcgaagtcg acggtcacga tctcgaagcg
     1021 gtgagcgcgg cgttgaccat cgcgcgtaaa gacccgcgac cgtcgctgct tgcgtgcaag
     1081 acgatcatcg gccgcggtat tgcgcgtttg caaggccagc gcggcggcca tagcggcaag
     1141 cttttccagg cggatgccga cgccgcccgc gagcaactcg gctggccgca tgcgccgttc
     1201 gacctgcccg cgcaagtgcg ggacgcatgg gtcgatgcgg gccggcgcag cagcgacgaa
     1261 tacatcgctt ggcacgcgcg tctcgctgcg ttgccagacg cgcagtgcgc cgagttcgaa
     1321 cgggtcctcg cgggcggctt gccgcaaggc tggcgcgacg tgctcgacgc ttaccgcaag
     1381 cgcgccgtcg aacgcgacga gccgcaaccg ggtatcaccg tgtcgggcga gatcagcgat
     1441 ctgttcgcgc cgctgctgcc cgagcgcatg atcggttgcg ccgatctcga ggcgcccacc
     1501 ggccacaaac gccagttgca cgccttcacc gccgacgacc gcagcggcgc ctacgttcac
     1561 tgcggcgtgc gcgaacatct gatgggtgcg atggccaacg gcatggcggc gcacggcggc
     1621 atcgtcgcga ccagcgtcac ctatctggcg ttctccgact accagcgtcc ggcgatgcgc
     1681 atggccgcgt tgatgggctt gccggtgcac ttcgtgttca gccacgactc catcggcgtc
     1741 ggcaagaacg ggccgacgca tcaacccgtc gagatcctcg cctcgttacg cgcgatgccg
     1801 aacatgcgcg tgctgcgtcc cgccgatgcc gtggaagcgg ccgagtgctg ggcactcgcg
     1861 ctcgaacatc gacacggccc gagtacgctg gtgttcgcgc gccaggcgtt gccgccggtg
     1921 cgccgcgagc atagcgccga accgttgtcg caacgcggcg cttacgtgct ggccgaggcg
     1981 caaggcggcc cgcgcgcagt gaccctgctc gcgaccggct cggaagtcgc gctggccttg
     2041 caggcgcgca gccagctcca ggacgagggc atcgcgaccg cggtggtgtc gatgccatgc
     2101 tgggaaatct tcgacgaaca ggacgccgat tatcgcgcca cagtcctggg tgtaaatacc
     2161 gtgcgtgtcg gagtcgaggc ggcgctgcgg ttcggctggg accgctatct cggcgagcga
     2221 ggcggttttg tcggcatgac aggcttcggc gcttccgcct ctgcggagac gttatatgag
     2281 cacttcggta tcacggcgca gcacgtcgtg gcggaagcga agcggcatct ataaaatcat
     2341 cgaggattct tcggagataa gcatgcaaaa aatcgttttt ctcgaccgcg ccacccttgc
     2401 gccgcaaatc gtattgcgcg agccgcgttt cgatcatcaa ctgatcgagc acgatcgcac
     2461 gttgcctgag caggtgttga cgcggctggc cggcgcatcg atcgcgatca ccaacaaggt
     2521 gccgctgacg aaagaagtgc tggcgcaatt gcccgatctg aaactcgtcg cggtggctgc
     2581 caccggcact gattgcgtcg acaaggcagc ttgccagcag ctcggcatcg ccgtgagcaa
     2641 tattcgcggc tacgcgatca acaccgtacc cgaacacacc ttcgcgctga tcctcgcgct
     2701 acggcgcaat ctcattgcgt atcgcgacga cgtgctcgcc ggcgagtggc agaagtccgg
     2761 gcaattctgt ttcttcaccc atccgattca cgatctggcc ggcgcgcggc ttggcatcat
     2821 cggcgaaggg gtgctcgggc agcgtgtcgc ggaaatcggc aaagcattcg gcatgcagcc
     2881 gatgttcgcc gctcataaag gacgtgacgg ccttggcccg ctttacacgc cgtgggacga
     2941 agttctcgcg accagcgaca tcatcaccgt gcacagtccg cttacgccgc acacgcgcaa
     3001 catgctggcc atgcccgagt tccgcgcgat gaagaagaag ccattgatca tcaacacggc
     3061 gcgcggcggt ctggtcgacg aagccgctct ggtgcaggca ctcgacgaag ggttgatcag
     3121 cggcgcgggt ttcgatgtga cggcaggcgg cgaaccaccg ccgccggaga gcccgctatt
     3181 gcgcgctgcc ggccgcccga atgtgatttt gacgccgcat gttgcatggg cctcggacga
     3241 ggcgcaacaa agtctcgccg atcagttaat ggataacatc gaaaatttcg tcgacggtgc
     3301 gccaacccat ctcgtgcaag gggcgtattg atttgacggg ggcgcggcgc cccgccgcgc
     3361 gtgaatcgtc ggatagtcgg atgaattgca accgcgacga tcgcatagag aactagctcg
     3421 ttgccgacaa aaagctgacc actccggacg agaaaaactg cctttcccca agcgaatgtt
     3481 tcatcacatc tagctgtggt ctccggtggc cgcgggcgcc gagaatgcgc gcggcttttc
     3541 ggactgtatt gctcggacca ggcgacctta cagcacagat ttccgtttcg cattagcata
     3601 cgagggtgtc ccgtgccgtt cggtggagta attcgccgga ccattgccgc gggaccgtca
     3661 acgtgtgcgg gtcgcccggt tcattcccca catctatcca tcgcaggctg acactgcgaa
     3721 cctgccgttt cacgtgcttt tcgagcggac ggccatcatt aatccaatcg tattgattga
     3781 acgaatcgtt ggttaatatg aggaatatac gtacttcatt gatagtcggc gtgcttgttg
     3841 tcgaggtggt gttcggcgtg tttttactgt cacacaaaag ccatcgcgcg acgaccgatc
     3901 ctgatgtcac gacaagcaac gcagtggaat tggctgcgac gtccccgcaa tcaggcgaca
     3961 cccatgtgac ggcgggcagt gtgatcagcg cagcgcctct ctccggcgac acagcaacag
     4021 cggcgaccgg cgatacagcc gccgcgaccg gcgctaaaca gtctacgagc ggggcagtcg
     4081 tcacggcatc cgactcaccg gccaaagacg ccgttcaggt gcctcgactg aacacgcgtg
     4141 cacgtgaatc cgtcgcgatt catgcttacg ccgggccgaa gaccggcgcg ttgccgcaaa
     4201 ccgaaatcgg acgtgacagt ctgcatcgtc acggctcgaa tccggtcggg gctgcgatca
     4261 cggatgcgct ggtcaaagag tccgccaaac tcgatccggc actcccgccg ccgacccaag
     4321 ccattcaacc gctccaacca atccaggcgg cccagccagc ccttgacgac tcataccatc
     4381 gcggctcgaa tcccgttgcc gccgccatga ctgaacaact cgtcagagaa tccgcgaaac
     4441 tcgatccggg actgccgccg ccgaatcaag ccagcatgaa gtagacgcgc gacgcgaagc
     4501 gtgggctact accggtag
//
LOCUS       DATASH010000895        10982 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1536874,
            whole genome shotgun sequence.
ACCESSION   DATASH010000895 DATASH010000000
VERSION     DATASH010000895.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 10982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10982
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1536874"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="VHV99_23905"
     CDS             <1..469
                     /locus_tag="VHV99_23905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004544347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEX3638464.1"
                     /translation="VGGTYGRSWGEVTTLLPWCVVAVPGFALLARPLDLLALGDDQAA
                     ALGLPIGVLRPLVLTVATLAACAAVAAVGPVGFIGLMAPHLASMLGARAHRTRLWLAA
                     ACGALVLVVADIAARTLLAPREIPAGVLTALIGAPYLLALLIVEARREKRGKR"
     gene            469..1308
                     /gene="fhuF"
                     /locus_tag="VHV99_23910"
     CDS             469..1308
                     /gene="fhuF"
                     /locus_tag="VHV99_23910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0033214 - siderophore-dependent iron import
                     into cell [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HEX3638465.1"
                     /translation="MGPRAGLSTIANRCGPHAQCLAAFAPESIAHYLAHVCLGTPDAS
                     DEPTLAENETGKPAVVAVTELASHRVALLDAMVKLYGGERETHARALLSQWSKYYFGF
                     AATAGFVAAAMLGRPLDMSPEKTHVVLRDGLPVALYFAQDALQAPASAPASRYTPLVD
                     HLNAVIETLAAMTKVAPRVLWSNAGNLLDYLFEQCTPLLDLADDAAWLFGPLAADGEV
                     NPLRMPVRLATPRAAGLPNPFSARRVCCVRYEIPGETQLCGRCPLLLTMSDAEIALQD
                     ASH"
     gene            1539..2474
                     /locus_tag="VHV99_23915"
     CDS             1539..2474
                     /locus_tag="VHV99_23915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX3638466.1"
                     /translation="MRATPLAQQTCTPLADSPSVSQASPSLPARPKRIVVLEFMFAED
                     LAALGITPVGMADPEYYPVWIGYDEARLASVPDIGTRQEPSLEAIAAIKPDLILGVGL
                     RHAPIFAALERIAPTVLFKYGPNFAEDGARVTQLDWGRTILRTIGCLTGREDAARAVE
                     AKVDAGFARDARRLAQAGRRGEQIAWLQELGLPDRYWAFTGNSTAAGVARALGLEPWP
                     AEPTREGTSYVTSEDLLKKPNLTVLFISATEPGVSLATKLDSPIWRFVPARSAGRVGL
                     VERNIWGFGGPMSALRMADTMTETLLSLPAPSASK"
     gene            2485..2616
                     /locus_tag="VHV99_23920"
     CDS             2485..2616
                     /locus_tag="VHV99_23920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638467.1"
                     /translation="MDCRYSTGLVREREEEREIKLRALEPLEYLGIARYAHCRTPGV"
     gene            complement(2653..4437)
                     /locus_tag="VHV99_23925"
     CDS             complement(2653..4437)
                     /locus_tag="VHV99_23925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic peptide export ABC transporter"
                     /protein_id="HEX3638468.1"
                     /translation="MTAANERYEASPSPSPSTAAHASAQAPGKPAMRLIVALLKRSRG
                     AFAIALTACVLNGIASVLLVATLSRALSLPGAADVSLAMRFALCAVLALVTRVITGVL
                     FARLSQDTMAQLREHVARRVAEAELRDVERIGAAPVQSVLTDDATNVSMLFFALPNLV
                     MHGSIVFGCLGYLAWLSWPVCLLALAAILVGSLGYHAGDKRAIASLEAAGRSQDKLFG
                     YLGALFTGAKELKLHQARSRQFLDGQLGSAIDEVRDHRRRAFSAYAVGVGWIVFLFYV
                     FLGVAAFWPALGVHADAPSASGYVVVFLFMLLPLDGLLNNVPTLNAARVSLDRIEKVM
                     SEFGALRTAPPPDENASHAPYQTLTLSNVTHSYFHERDERMFRVGPVNLTFRPGELVF
                     IVGGNGSGKTTLAKVLTGLYEPEDGTIELDGKPVGFAERAAYRQRFTAVFNDFHLFDA
                     LLGIVDPNDASRAQADARANALVAKLALDHKVQVVNGAFSTRALSTGQRKRLALVVAY
                     LEDRPFYLFDEWAADQDPQFKAVFYAQLLPELRSRGKTVLVISHDDRYFHLADRVLKL
                     DNGSIVSETYGVAQAVENKCAVANGTVR"
     gene            4731..>10982
                     /locus_tag="VHV99_23930"
     CDS             4731..>10982
                     /locus_tag="VHV99_23930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006026683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEX3638469.1"
                     /translation="MTSFPIALHHRIRALARIEPDAPALASFTPHTVRLTRGELDRRA
                     ARLAAQLRAAGVTTEVRVGVCVARSCDLFVALLAVLKAGGVFVALDPRHPAARLDWVA
                     RDAGLAHGIVDASADAVMRARFAQCFDVASDALAEVEVEVEVEAEAEAEAEAEAKADA
                     DVAAAADAARFDGDDEPVHPRAAAYMIYTSGSTGKPKAVAVEHGPLAAHGDALAESLP
                     IDGSDRVLHFASVNFDVSIEAWLVPLAVGGSVVISDPPPFAPEATHAFMLREGVTNTT
                     LPPAYLREFANVCERLGVPPSLRVLLFGGEAMSQDSFDEIRRVFPSIRLVNGYGPTEA
                     VISPMLWPVDPGTTPARHAGNGYASLPIGWPIGRRVARIEGAARRGEPGELLLGGVCL
                     ARGYHGRAALTAERFLPDANGAPGERIYRTGDLAREREDGSFDYLGRIDDQVQVRGVR
                     VEPGEIAACLLTHPAIADAGVLAETAGGRTQLIACVVLKEVAAAQVRGEAAHGDATRS
                     DATRSDAPRSDATHGDATHSDVTRSDAPHSDATHGDAVCGEKPRDAAASSDAALQAHL
                     AAHLPQAWLPHRFARFARLPYTLNGKLDRAALRDAVAAVPVASAADYVSPQTVTEQRL
                     ADIWQTLLNEPTPIGRADRFFSRGGDSLAAMQLQAAIRIEWRVNLRLDALFDDQALET
                     LAALIDNSETETAVNHPQRITVLQPATAAFVDRAASFAQQRFWVLAQTQDAGSAYHVA
                     VQWDVQGALDAGTLQRALDCLIERQQAWRTTLVENDDGIVMQRIHAELPVQIAEIELH
                     TSSEVERASRLAALMEQHVGAAFDLSNGPLVRATLITLTNNAQRFLLTTHHAISDGWS
                     SRCAFGELQAAYTAFATGRAPLLPALAVQYADYAQWQRDWLAAGEGERQLAYWRDALR
                     AAPEPLALPLDRPRSPQRDYRGGRLALRLPQAVSAAVRETARRAQASPFTVLLAAFDA
                     WLYRLTGATDIVVAAPIAHRQRAETAPLVGLFLNTLALRARVAPGQSFAALLDDVRRS
                     AFDAFAHQDVPFDQVLDAVKPPVRRGEEWLKVKFAQQFDLELTAELPGASVRLSAGLD
                     LAARFDLALDFTDDPRGIELVAAYALDGLDEATAQAWLDSYGTLVADAVRDPRRLIAE
                     LDCADVVGYGTARYGRALQHEADTVLALFARHASETPHRIALADADTQLTFAELDDAS
                     ERIALALQQRKVGAEQPVAVCIERSVHFAVALLGVMKSGAYAVPLDPVAPHERLAAAV
                     DACGAHWMLTAGGQQAIQAVGAAKTLDIDALTQCASSLSTHADASAQVGVNAKAESAA
                     NVEVHAADTPRPAPLPDQAAYLIFTSGSTGMPKGVVISHRALADYVVGMLDELAFAAD
                     ASMAMVSTVAADLGHTTLFGALCSGRTLHLLPAQCAFDPDRFAHEMRTRDVGVLKIVP
                     SHLHALLDAQNPADVLPTHALVTGGETLLWSLVARIAALKPACRVINHYGPTEATVGA
                     LTFDTSAPAQAGLRLPEVLAEQGGHFTRAAQSTQASQFTQSTQFTQATHAAQSTQSTQ
                     TAQTVPLGLPLPNAYACVLDSHGASVPPGGIGELYLGGPGLARGYLNRAAATAERFVP
                     HQFAPGERLYRTGDRVRLRADRRLDFLGRLDEQVKIRGYRVEPGEVSAALRALEGVAQ
                     AETLAVEHEGRLRLASFVASSNGARLDESALRAALTARLPDYMVPAVLLILAALPVTA
                     NGKVDRAALRALAVTPRPSVAEGDAPQGATEEALAAVWKEVLKAERVGRDDNFFELGG
                     DSILVLQVIARSRKRGVRFSPKQLFDAPTLAQLACVATTADAAAPIASKAQSATPASR
                     TQVSQAASRQATTLTPAQLRFFALDIPQRGHWNQSIELETQAPFDADAFARAFETLLT
                     HHEIFRHRFTLNGPREEWRLTFATRAFEALPLAVAAARDESDALAQFDALQSTLDLTY
                     GPLVCAFAALLPDGTTKLYLAIHHLIVDGVSWRVLLDDLDAAYRAARERRTIRLASTG
                     TNAQEWAARLARAALDATSSPIVTGVPHSAAFEATNSPFAADVPCSATPDATHSPFAT
                     EVPYWTALAA"
BASE COUNT         1895 a         3498 c         3637 g         1952 t
ORIGIN      
        1 ggtcggcggc acgtacgggc gcagctgggg cgaagtgacg acgctgctgc cgtggtgcgt
       61 ggtggcggtg cctgggttcg cgctgctggc acggccgctc gatctgctcg cgctaggcga
      121 cgaccaggcc gccgcgctcg ggttaccaat cggcgttttg cgtccgcttg tgctgacggt
      181 ggcgacgctg gccgcctgtg ccgcggtggc cgcggtcggg ccggtaggtt ttatcggttt
      241 gatggcgccg catctcgcat cgatgcttgg ggcgcgtgcc catcgcacgc gtctatggct
      301 tgctgccgcg tgcggtgcgc tggtactggt ggtcgccgat atcgcggcgc gcacgctgct
      361 cgcgccgcgc gagattcctg cgggcgtgct gacagcgttg atcggcgcgc cttacctgct
      421 ggcgttgctg atcgtggagg ctcggcgcga gaagcgaggg aaacggtgat gggaccacga
      481 gccggactat caacgatcgc aaacaggtgc ggcccgcacg cgcagtgcct cgccgctttc
      541 gcgccggaat cgatcgcgca ctatctggcg cacgtgtgcc tcggcacgcc tgacgcgagc
      601 gacgaaccga cgctcgcgga gaatgaaaca ggcaagccgg ccgtcgtcgc tgtaacggaa
      661 ctagcgagcc atcgcgtcgc cttgctcgat gccatggtca agctctatgg cggcgagcgc
      721 gaaacgcacg cacgtgcgtt gctctcgcaa tggagcaagt actacttcgg cttcgcggca
      781 accgctggct ttgtcgctgc ggcgatgctg ggccgtccgc tcgacatgtc gccggaaaag
      841 acgcacgtgg tgctgcgaga cggtctgcct gtcgcgcttt atttcgctca agacgcattg
      901 caagcacccg ccagcgctcc cgcgagtcgc tacacaccgc tcgtcgatca tttgaacgcg
      961 gtgatcgaga ccctcgctgc gatgacgaaa gtcgcgccgc gcgtgttgtg gagcaacgcg
     1021 ggcaatctgc tggattatct gttcgaacaa tgcacgccac tattggactt agccgacgac
     1081 gccgcctggc tcttcggccc gctcgccgcc gatggcgagg ttaatcctct gcgcatgccg
     1141 gtgcgtcttg ccacgccacg cgccgcgggc ttgccgaatc cgtttagcgc acggcgcgtg
     1201 tgctgcgtac gttacgaaat tcctggagag acgcaactgt gtggacgctg ccccttgcta
     1261 ttgacgatga gcgatgccga aatcgccttg caggacgctt cgcattgacg ggccgtgcga
     1321 gaaaaccgca acagttcaag cgcgtgcgtg cggctacggc agtggcagtc gcgctgcttg
     1381 ccaactcata tgcgttcgcg cagagcggtg ccggcgatgc aaccggcgcg acgaacgcaa
     1441 accgcgttgt caaacctgca ggcgagaaca acgcaagtaa cgcaactagc tttaacggtg
     1501 caaatggcga aagcactgct actggcgaag ccagggacat ccgcgccact ccgctcgcgc
     1561 agcaaacctg cacgccactc gccgacagtc caagcgtctc gcaagcaagc ccctcgttgc
     1621 ccgcacgacc gaagcgcatc gtcgtgctcg aattcatgtt cgccgaagac ctcgccgcgc
     1681 ttggcatcac cccggtcggc atggcggacc ccgagtacta cccggtgtgg atcggttacg
     1741 acgaagcacg ccttgcatcg gtgccggaca tcggcacgcg ccaggagccg agcctcgaag
     1801 caatcgcggc gatcaaaccg gacctgatcc tcggcgtagg cctgcgtcat gcgccgatct
     1861 ttgccgcgct cgaacggatc gccccgaccg tgctattcaa atacggcccg aactttgccg
     1921 aagacggcgc ccgcgtgacg caactcgatt ggggccgcac cattctgcgg acgatcggct
     1981 gcctgacggg acgtgaggac gcggcgcgcg ctgtcgaggc caaagtggat gcaggcttcg
     2041 cccgcgatgc gcggcgcctc gcgcaagccg gccgtcgagg cgagcagata gcgtggctgc
     2101 aggagttggg attgcccgac cgatactggg cctttaccgg caacagcacg gcagccggtg
     2161 tcgcacgcgc gcttgggctc gagccgtggc ccgcggagcc gacgcgggaa ggcacgtcct
     2221 atgtgacgtc ggaggatctg ttgaagaagc cgaacctcac ggtgctgttc atcagcgcga
     2281 ccgaacccgg cgtgtcgctc gcgaccaaac tggattcgcc gatctggcgc ttcgtgccgg
     2341 cccgtagcgc ggggcgtgtg ggtctcgtcg aacggaatat ctgggggttt ggcgggccga
     2401 tgtccgcgct gcggatggcc gatacgatga cggaaacgct gctatctctg ccggcgccgt
     2461 ccgcgagcaa gtaagcggtt gcaaatggat tgccgttatt cgaccggctt ggtccgtgag
     2521 cgcgaggagg aacgcgagat caagctgcgt gcgctcgaac cgctcgagta cctcggcatt
     2581 gcgcgatatg cgcattgccg cacgccgggc gtttgagcaa acacacccgg cgtggatctc
     2641 acgtaactca acttatctca ccgtgccgtt cgcaacggca cacttattct ccacggcctg
     2701 cgccacccca taagtttcgc tgacgatact gccgttatcc agcttcaaca cacgatccgc
     2761 gagatggaag tagcggtcgt cgtggctgat caccagcact gtcttgccgc gcgaacgcaa
     2821 ttccggcagc agttgcgcat agaacaccgc cttgaactgc gggtcctgat ccgcggccca
     2881 ttcgtcgaac aggtaaaacg gccggtcttc cagatacgcg acgactagcg ccaggcgctt
     2941 tctctgaccg gtggagagcg cgcgtgtcga gaatgcgccg ttcaccacct gcaccttgtg
     3001 gtcgagcgcg agctttgcaa ccagcgcatt ggcgcgggcg tcggcctgtg cgcgcgatgc
     3061 atcgttcgga tcgacgatgc cgagcagcgc gtcgaacaga tgaaagtcgt tgaacacggc
     3121 ggtgaagcgc tgccggtacg cggcgcgttc ggcgaagccg accggcttgc cgtccagttc
     3181 gatcgtgccg tcttccggtt catacagacc cgtgagcacc ttcgcgagcg tcgtcttgcc
     3241 gctgccattg ccgccgacga tgaacaccag ttcaccaggc cggaaagtca gattgaccgg
     3301 cccgacgcgg aacatccgct catcgcgttc atggaagtac gagtgcgtga cgttgctcag
     3361 cgtgagagtc tggtacggcg cgtgcgaggc gttttcatcc ggcggcggtg cggtgcgcag
     3421 cgcgccgaac tcgctcatca ccttttcgat gcggtcgagc gacacgcgcg ccgcattcag
     3481 cgtcggcacg ttgttgagca gaccgtccaa cggcagcagc atgaacagga acaccaccac
     3541 atagcccgaa gccgacggcg catcggcatg tacaccaagc gcgggccaga acgccgcgac
     3601 gcccagaaac acgtagaaca gaaacacgat ccagccgacc ccgaccgcat aggcgctgaa
     3661 cgcgcggcgc cgatggtcgc gcacctcgtc gatcgcggaa ccgagttggc cgtcgaggaa
     3721 ttgacgcgag cgcgcctgat gcagtttcag ttcctttgcg cccgtaaaca gcgcgccgag
     3781 atagccgaac agtttgtcct gcgagcggcc cgcggcttcg agcgaggcga tggcccgctt
     3841 gtcacccgcg tgatagccga gcgagccgac cagaatcgcg gcgagcgcga gcaggcagac
     3901 cggccacgac aaccacgcca gatagccgag gcagccgaac acgatcgagc cgtgcatcac
     3961 gagattgggc agcgcgaaga acagcatcga gacgttggtc gcatcgtcgg tcaggaccga
     4021 ctgcacgggc gccgcgccga tccgttcgac gtcgcgcagt tcggcctcgg cgacgcgccg
     4081 cgcgacgtgc tcgcgcaact gcgccatcgt gtcctgcgac agacgcgcga acagcacgcc
     4141 ggtaatcacg cgcgtgacga gcgcaagcac cgcgcataac gcaaagcgca tggcgagcga
     4201 cacgtcggcg gcgccgggta gcgacagcgc gcgactcaag gtcgccacga gcagcacgct
     4261 ggcgatgccg ttcaacacgc aggcggtgag cgcgatcgcg aaggcgccgc gcgagcgctt
     4321 gagcagcgcc acgatcaggc gcatggccgg cttcccgggc gcctgagcgg aggcgtgagc
     4381 ggcggttgag ggagaaggcg agggtgacgc ttcgtagcgc tcgttggcag cggtcatcgg
     4441 caatgggtcg cagtgggtcg cagtggatcg gtgagatgca gcgcatgcgg gcggcaaatc
     4501 ggcggggcgc aaagacgcgc ccctcgcctg atagacgaac gggcgttgca aacatttacc
     4561 gtctggcgcc gattggctct gcttacgacc gtccgtgact gaaaagatgg agtccgaata
     4621 aatagacggc ggcggtaaag attttcgatt gcggatcgtc acaccaatga agcggcccat
     4681 tgcggcctgc gacttggccg acgcggccag ttctaacgga tttcacagcc atgacgagct
     4741 tcccgattgc cctgcatcat cgaatccgcg ctttggcgcg catcgaaccc gacgcgcccg
     4801 cgcttgcatc gttcacgcca cataccgtgc ggctgacacg cggtgagttg gaccgccgcg
     4861 cggcgcggct tgccgcacaa ctgagggcag cgggcgtcac gacggaggtg cgcgtgggtg
     4921 tgtgtgtcgc gcggtcgtgc gatctgttcg tcgcattgct ggcggtgctg aaggcgggcg
     4981 gggtgttcgt cgcgctcgat ccgcgtcatc cggcggcgcg cctcgactgg gttgcgcggg
     5041 atgcggggct cgcacatggc attgtcgacg cgtccgccga tgcggtaatg cgcgcgcgtt
     5101 tcgcgcaatg cttcgacgtc gcaagcgacg ctttggcgga ggtggaggtg gaggtggagg
     5161 tggaggcgga ggcggaggcg gaggcagagg cagaggcgaa ggcagacgca gacgttgccg
     5221 ctgccgcaga cgcggcgcgt ttcgatggcg atgacgagcc ggtgcatccg cgcgcggccg
     5281 cttacatgat ctacacgtcc ggttcgacgg gcaagccgaa ggcggtggcc gtcgaacacg
     5341 gaccgcttgc cgcgcacggc gacgcgctcg ccgaatcctt gccgatcgac ggcagcgatc
     5401 gcgtattgca tttcgcctcg gtgaatttcg atgtgtcgat cgaagcatgg ctggtgccgc
     5461 tcgccgtggg cggcagtgtg gtgatcagcg atccgccgcc gtttgcgccg gaagccacgc
     5521 atgccttcat gctgcgcgag ggtgtgacca acaccacgct gccgccggct tatctgcgcg
     5581 aattcgccaa cgtttgcgag cggctcggcg tgccgccttc gttgcgcgtg ctgctgttcg
     5641 gcggcgaagc gatgtcgcag gacagcttcg acgaaattcg ccgcgtgttt ccgtcgattc
     5701 gtctcgtcaa tggctatgga ccgaccgagg cggtgatttc gccgatgctg tggccggtcg
     5761 atcccggcac gaccccggcg cggcacgcgg gcaacggtta tgcgtcgctg ccgatcggct
     5821 ggccgatcgg gcgccgggtc gctcgaatcg agggtgcggc gcggcgaggt gagccaggcg
     5881 aactgctgct tggtggcgtg tgcctcgcgc ggggttatca cggccgtgcg gcgctcaccg
     5941 ccgaacgttt tttgccggac gcaaacggcg cgccgggtga gcggatctac cgcaccggtg
     6001 accttgcgcg tgaacgcgag gatggatcgt tcgattatct cggccgcatc gacgatcagg
     6061 tgcaggtgcg cggcgtgcgg gtggagccgg gtgagatcgc ggcatgtctg ctgacgcatc
     6121 cggcgatcgc cgatgcgggc gtgcttgccg agacagcggg cggcagaacg caactgattg
     6181 cctgtgtggt gctgaaagag gtggcggccg cacaggtacg tggcgaagca gcgcacggcg
     6241 atgctacacg cagcgatgca acacgcagcg atgcgccacg cagcgacgca acacacggcg
     6301 atgcaacaca cagcgatgta acacgcagcg atgcgccaca cagcgacgca acacacggcg
     6361 acgctgtatg cggcgaaaag ccccgcgacg cagcagcatc cagcgacgct gcattgcagg
     6421 cgcacctcgc tgcgcatttg ccacaagcgt ggctgccgca tcgcttcgcg cgctttgccc
     6481 gtctacccta cacgctgaac ggcaagctcg atcgcgctgc tttgcgcgat gccgtggcgg
     6541 cagtgccagt tgcctccgca gccgactatg tctcgccaca aaccgtcaca gagcaacgcc
     6601 ttgctgatat ctggcaaaca ctcctgaacg agcccacgcc gatcggtcgc gccgatcgat
     6661 tcttttcgcg cggcggcgat tcgctcgcgg cgatgcagtt gcaggcggcg atccggatcg
     6721 agtggcgtgt gaatttgcgg ctcgatgcct tgttcgacga tcaagcgctc gagacactcg
     6781 cggcgctgat cgacaacagt gaaacggaaa ccgcagtgaa tcaccctcag cggatcacgg
     6841 tactgcagcc tgcaactgcc gcgttcgtcg accgtgctgc ttctttcgcg cagcagcgct
     6901 tctgggtcct tgcgcagact caggacgccg gctcggcgta tcacgttgcc gtgcaatggg
     6961 acgtgcaggg cgcgctcgat gccggcacat tgcaacgcgc gctcgattgt ctgatcgagc
     7021 gtcaacaggc ctggcgcacg acgctcgtcg aaaacgacga tggcatcgtc atgcagcgca
     7081 ttcatgccga gttacccgtg caaattgctg aaatcgagct gcataccagc agtgaggtcg
     7141 agcgcgcgtc gcggctcgcg gcgttgatgg agcaacacgt cggcgcagcg ttcgatctat
     7201 cgaacggtcc gctggtgcgc gccacgctca tcacgctgac caacaacgcg caacgcttcc
     7261 tgttgacgac ccatcatgcg atcagcgacg gctggagctc gcgttgtgcc ttcggcgaac
     7321 tacaggcggc ctacacggca ttcgcgacag gacgtgcacc gctattgccc gcgttagcag
     7381 tccaatacgc cgattatgcg caatggcagc gcgactggct cgccgcaggc gaaggcgaac
     7441 gacagctcgc gtattggcgc gacgcgttgc gtgccgcgcc ggaaccgctg gcgttgccgc
     7501 tcgaccgccc gcgttcgccg caacgcgact atcgcggcgg ccggctcgcc ttgcggctgc
     7561 cgcaggccgt ctctgccgcg gtacgcgaaa ccgcgcggcg cgcgcaggcg tcgcccttca
     7621 ctgtgttgtt ggccgctttc gacgcctggc tctatcggct gaccggcgca accgacatcg
     7681 tggtggccgc gccgattgcg catcggcagc gtgcggaaac cgcaccactg gtcggtctgt
     7741 tcctcaacac gctggcattg cgcgcgcgtg tggcgccggg ccagtcgttc gctgcattgc
     7801 ttgacgacgt acgccgctcg gcgttcgatg ctttcgcgca tcaggatgtg ccgttcgatc
     7861 aggtgctcga cgccgtgaaa ccgccggtgc ggcgcggtga agaatggctc aaggtcaagt
     7921 tcgcgcagca gttcgatctg gaattgacgg ctgaattgcc gggtgcgtcg gtgcggctgt
     7981 cggcggggct cgatctcgct gcgcgcttcg atctcgcgct tgatttcact gatgatccgc
     8041 gcggtattga actggtggcc gcttatgcgc tcgacggtct cgatgaagcg accgcacagg
     8101 cgtggctcga tagttatggc acgctcgttg ccgacgcggt gcgcgatccg cggaggttga
     8161 ttgctgaact cgactgcgcg gatgtggtgg ggtatggcac ggcgcgatat gggcgcgcgc
     8221 ttcagcatga ggcggacacc gtgctcgcgc ttttcgcgcg tcacgcgagt gaaacgccgc
     8281 atcgcatcgc gctggcggac gctgacacgc agctcacgtt cgccgaactc gacgatgcgt
     8341 cggagcgcat tgcgcttgcg ttgcagcaac gcaaagttgg cgcggagcag ccggttgcgg
     8401 tgtgtatcga gcgttcggtg cattttgcgg ttgcgttgct cggcgtgatg aagtccggcg
     8461 cgtacgcggt gccactcgat cccgttgcgc cgcacgagcg gttggcggct gccgtggatg
     8521 cctgcggcgc ccattggatg ctgaccgcgg gcggccagca agccatccag gccgtaggtg
     8581 ctgcaaagac gctcgatatc gacgcgctca cgcagtgcgc gtcgagtttg agcacacatg
     8641 ccgacgctag tgcgcaggtg ggagtcaacg cgaaagcgga gtcggcggcg aacgtggagg
     8701 ttcacgccgc cgacaccccg cgtccggcac cgctgcccga ccaggcggcc tatctgatct
     8761 tcacatcggg atcgaccggc atgcccaagg gcgtcgtgat ctcgcatcgc gcgctggctg
     8821 actacgtcgt aggcatgctg gacgaactcg cctttgccgc cgacgcttcg atggcgatgg
     8881 tctccaccgt cgccgccgac ctcggtcaca cgacgctatt cggtgcgctc tgctcgggcc
     8941 gcacgctgca tctgttgccg gcccaatgcg cgttcgatcc tgatcgcttc gctcatgaga
     9001 tgcgcacgcg cgacgtcggg gtgttgaaga tcgtgccgag ccatctgcat gcgctgctcg
     9061 atgcacagaa ccccgccgac gtgttgccca cgcacgcgtt ggtgacgggc ggcgagacgc
     9121 tgctgtggtc gctggtcgca cggatcgcgg cgcttaaacc ggcgtgccgt gtcattaatc
     9181 attacggccc gaccgaggcg acggtcggtg cattgacctt cgatacatcg gcgccggcgc
     9241 aagctggatt gcgcttgcca gaggttctcg ctgagcaagg gggacatttc acgcgtgctg
     9301 cgcaatccac gcaggccagc caattcacgc aatccaccca attcacgcag gccacgcacg
     9361 ccgcgcaatc cacgcaatcc acgcaaaccg cacaaaccgt ccccttaggc ctgccgttgc
     9421 caaacgcata cgcatgtgtg ctcgacagcc acggcgcaag cgtgccaccc ggcggaattg
     9481 gcgaacttta cctcggtggt ccgggccttg cgcgcggcta tttgaaccgc gctgccgcca
     9541 ccgccgagcg cttcgtaccg catcagttcg cgccgggcga gcgtctttac agaacgggcg
     9601 accgcgtgcg gctgcgtgcc gatcgtcggc tggattttct tgggcggctc gacgaacagg
     9661 tgaaaattcg cggctatcgc gtcgaacccg gtgaagtcag tgcggcgctg cgtgcgctcg
     9721 aaggcgtcgc tcaggcggaa acgcttgctg tggagcatga agggcgtttg cgcttggcat
     9781 cattcgttgc ctcgtcgaac ggcgcacgtc tcgacgaatc ggctttacgc gcagcattga
     9841 ccgcgcgtct gccggactac atggtgccgg ctgtgttgct gatcctcgcc gcgttaccgg
     9901 tgaccgcgaa cggcaaagtc gatcgagcgg cattgcgcgc gcttgctgtg acgccaaggc
     9961 catccgtcgc tgaaggtgac gcgccgcaag gcgcgacgga agaggcgctc gccgccgtct
    10021 ggaaggaagt gctcaaggct gaacgggtgg gccgcgacga taacttcttc gaactcggcg
    10081 gcgattcgat tctggtgtta caggtgatcg cgcgctcacg caagcgcggt gtgcgctttt
    10141 cgccgaagca gttattcgac gcgccgacgt tggcgcaact cgcctgtgtt gcaacaacgg
    10201 cggatgccgc ggcgccgatt gcatcgaagg cgcaatccgc gacgccggca tccagaacgc
    10261 aggtatcgca ggcagcgtcc cggcaagcca cgacgctcac cccggcgcaa ctacgtttct
    10321 tcgcgctgga tatcccacag cgtggccact ggaatcaatc gatcgaactc gagacccagg
    10381 cgccgttcga cgctgatgct tttgctcgcg cattcgaaac actgttgacg catcacgaga
    10441 tcttccgtca tcgttttacg ctgaacggtc cccgtgaaga atggcggctc acattcgcca
    10501 cgcgcgcgtt cgaggcacta ccgcttgcag tcgctgccgc gcgcgatgaa agcgacgcgc
    10561 ttgcccagtt cgatgcgttg caaagcacgc tcgatctgac ctacggcccg ctagtttgtg
    10621 cgttcgccgc gttgttgccg gacgggacga cgaagcttta tctggcgatt catcacctga
    10681 tcgtcgacgg cgtgtcgtgg cgcgtgttac tggacgatct cgatgccgcc tatcgtgcgg
    10741 cgcgcgagcg ccgcacgata cgtcttgcat cgacgggcac gaacgcgcag gaatgggcgg
    10801 ctcggctcgc gcgcgcggcg ctcgacgcaa caagctcgcc gatcgtcact ggtgttcctc
    10861 attcagccgc attcgaagca acgaactccc cgttcgccgc cgacgtgccg tgttcagcca
    10921 cgcccgacgc aacacactca ccattcgcca ccgaagtgcc ttattggacc gctctcgcgg
    10981 cg
//
LOCUS       DATASH010000896         3912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2328746,
            whole genome shotgun sequence.
ACCESSION   DATASH010000896 DATASH010000000
VERSION     DATASH010000896.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2328746"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..469
                     /locus_tag="VHV99_23935"
     CDS             92..469
                     /locus_tag="VHV99_23935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638470.1"
                     /translation="MSNAINTVGGSMPFDMGNLPDAAQNGIQTDASFSTVRMFSPDDT
                     GGAGNMPPAEMAHARHAAANDGDFDTQGAGTQGGGGGGADMAGAMSQIMDLIKTAISA
                     AMPLLGMVTSMMGKAGAAGGGAA"
     gene            737..1348
                     /locus_tag="VHV99_23940"
     CDS             737..1348
                     /locus_tag="VHV99_23940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638471.1"
                     /translation="MNSSPRLGPVDRHRLDDLVRRSNAGLYSAMSHFVDGGHRPIGES
                     LRSGALPDAVVQRFLDALQSMQDYVGTSYRVSRITRDGLAALQGDRGGRFVDLGAQCA
                     FVLPSSAATWAEHPAAAQRRDGAYPLFSIFDESVPQKNLSTALYADCVAVLPGTPLRL
                     KSLRIVAAHGERGPGRARIVAHFAAADQACGAPYDLYSGAPRK"
     gene            1552..2445
                     /locus_tag="VHV99_23945"
     CDS             1552..2445
                     /locus_tag="VHV99_23945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011549404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX3638472.1"
                     /translation="MNRYALLLITSMLLVGSNVGIGKSIVAFVPVPLFALLRFVIAMA
                     VLWPLLRIAKLRRVKRGEWLNLFLQALFGTFGFTLLMLGGVQRTSAVAAGVITSTIPA
                     VVALLSWLILKERPNGRALASIGLAIVGVVVINLAHGDEHVGGEASFTGNLMVLGAVC
                     CESLYIILSRRLTQTLAPIDICAYTHMFGLLLMLPFGATALLHFDYRAVPLSVWTLVL
                     WYGLSASIFSFWLWMKGIRHVPGSLAGVFSAVLPVAAAIYGIVFLGERPTPAHGIALA
                     CVVAGIGLASLKVKRVPPVAS"
     gene            2667..2789
                     /locus_tag="VHV99_23950"
                     /pseudo
     CDS             2667..2789
                     /locus_tag="VHV99_23950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010107934.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
     gene            2922..>3912
                     /locus_tag="VHV99_23955"
     CDS             2922..>3912
                     /locus_tag="VHV99_23955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEX3638473.1"
                     /translation="MTVIKQEDLIQSIADSLQYISYYHPLDYIQALGRAYELEQSPAA
                     KDAIAQILTNSRMCAEGKRPICQDTGIVTVFVKVGMDVRWDGATMGVTEMINEGVRRG
                     YLNPDNVLRASIVSPPEGARKNTTDNTPAVIHYEIVPGDKVDVQIAAKGGGSENKSKL
                     AMLNPSDSIVDWILKTVPTMGAGWCPPGMLGIGIGGTAEKAMVMAKESLMEPIDIQDV
                     IARGPQDWIEELRVELHEKVNALGIGAQGLGGLATVLDVKIMAAPTHAASKPVAIIPN
                     CAATRHAHFTLDGSGVAKLEAPSLDAWPKVQWEPNTETSKRVDLNTLTPEEVAG"
BASE COUNT          673 a         1281 c         1223 g          735 t
ORIGIN      
        1 cgggaatctg acaaccgtgg agcaccggct gtgattgttc gcgctaaaac ggcaaccgaa
       61 cggtttaact tttaatcacc aggatttcac aatgagcaat gcaatcaaca ccgtcggcgg
      121 cagcatgccg ttcgatatgg gcaatttgcc ggacgcggcg caaaacggca tccagaccga
      181 cgcaagcttc agcaccgtgc ggatgttttc ccccgacgac accggcggcg ccggcaatat
      241 gccgccggcc gagatggcgc acgcccggca cgcagccgcg aacgacggcg acttcgacac
      301 ccagggcgcg gggacccagg gcggcggcgg tggtggcgcc gacatggcgg gcgcgatgag
      361 tcagatcatg gacctcatca agacggccat ttcagccgcg atgccgctgc tcggcatggt
      421 cacgtcgatg atgggcaaag cgggtgcggc cggaggcggc gccgcttaac ggaagcctga
      481 ggtcacggcc ggccgtcgca gcgacgccgg ccggaagcgg ggcggcgcgt cagcaagccg
      541 ctccgcttcc tgtccccttc atgcccctgc ttcgtaggtc cgcttcatag ccagcttcat
      601 agccagcttc gtagccagct tcgtagccag cttcgtagcc cgcttcgtag cccgcttcgt
      661 agcccgcttc ctagccagct ttaatagccc gctccatagc ctcgctccct gctccgaact
      721 tccctcttag aacatcatga actcttcccc aaggctcggc cccgtcgatc gccatcggct
      781 cgacgacctc gtgcggcgct ccaatgcagg gctctacagc gccatgagcc atttcgtcga
      841 cggcggccat cgccctatcg gcgagtcgct gcgcagtggc gcattgccag atgcggtcgt
      901 gcaacgcttc ctcgatgcac tgcaatcgat gcaggactat gtcggcacct cgtaccgcgt
      961 gtcgcggatc actcgcgacg ggcttgccgc gctgcaaggc gaccgcggcg ggcgctttgt
     1021 ggacctcggc gcccagtgcg ccttcgtgct gccgtccagc gcggccacct gggcagagca
     1081 cccggctgcc gcgcaacggc gcgacggcgc gtacccgttg ttctcgatct tcgacgaatc
     1141 ggtcccgcag aagaatctgt cgaccgccct ctacgcggat tgcgtcgccg tgctgccggg
     1201 tacgccgctg cgcctgaaga gcttgcgcat cgtcgcagcc cacggcgaaa ggggccccgg
     1261 acgcgcgcgt atcgttgcgc attttgccgc ggcggatcag gcatgcggtg cgccatacga
     1321 tctgtacagc ggcgcgccgc gcaagtgacg cggttgataa cttcgatacg tttgacacct
     1381 ttgacacgag gcagatacct tgaacacgtt tgataccttc aacgcggttg ataccttcga
     1441 cacgttcgac gcatttgacg cgaggcaagc ggcgggcgcg cgagcaggtg cggggcgccg
     1501 gccagtccgg tagactatgc gccgacgcac gctcttatcc ggatcctcag cttgaatcgc
     1561 tacgccctgc ttctcattac ctcgatgctg ctggtcggca gcaatgtcgg aatcggtaaa
     1621 tcgatcgtcg cattcgtccc cgtccccctg ttcgcgctgt tgcgcttcgt gattgcgatg
     1681 gcggtgttgt ggccgctgtt gcgcatcgcc aagctgcgcc gcgtcaaacg gggtgaatgg
     1741 ctcaatctgt tcttgcaggc gctgttcggc accttcggtt ttacgctgct gatgctgggc
     1801 ggcgtgcagc gtaccagcgc ggtcgcggcc ggcgtcatca ccagcacgat tcccgctgtc
     1861 gtcgcgctgc tctcgtggct aatcctcaaa gagcgtccga acggtcgggc gctcgcatcg
     1921 atcgggctgg cgatcgtcgg cgtggtcgtc atcaatctcg cgcatgggga cgagcacgtc
     1981 ggcggcgagg cttcgttcac cggcaacctg atggtgctgg gcgccgtgtg ctgtgaatcg
     2041 ctttacatca tcctttcgcg tcgtctcacg caaaccctcg cgccgatcga catctgcgcc
     2101 tacacgcata tgttcggcct gctgctgatg ctgccgttcg gcgccaccgc gctgctgcac
     2161 ttcgactatc gcgcggtgcc gctcagcgtg tggacgctgg tgctatggta cgggctgtcg
     2221 gcgagcatct tttcgttctg gctgtggatg aagggcattc gtcatgtgcc gggcagtctc
     2281 gcgggcgtgt tcagcgccgt gctgccggtg gccgcggcga tttatggcat cgtctttctc
     2341 ggcgagcggc ccacacccgc acacggcatc gcactcgcct gcgtggtcgc ggggattggg
     2401 ctggcgagcc tgaaggtcaa gcgggtgccg ccggtagcct cctgaatacc gctgcctgtt
     2461 accaggagcc gctgcccgcc gcgccgtagc aaattcagca gtcgctgtac aatagcgcgc
     2521 ccgacgtaag ctttccggcc tgccttggcc gtgcaccgcg ccgtctgcgc cccggtttgc
     2581 atgcttgacc cgcgcgtttg atctgagcgc cgggtcggca catttggtcc gtgttccgtc
     2641 acctgcgctt catctgactt ttgcctatgt ccgctccgcg acccgactcc gcccgtcagc
     2701 gccgcccaat gcacccgctg cccgtcatca ttttcatgag ccgctggctg cagatgccgc
     2761 tctatctggg cctgatcgtc gtccaggctg cgaaatattt tcaggaaccc gcggctttgc
     2821 acgcgctggg tttgaacgct ttccctcccc tgaaggcgca cgactgaccg cactgtcccg
     2881 cgagtctgaa aacaagcgcc actgtcccca ctgagctagc catgaccgtc atcaaacagg
     2941 aagatctgat tcagagcatt gctgattcgc ttcagtacat cagctattac cacccgctcg
     3001 attacatcca ggccttgggc cgcgcttacg agctcgaaca gagcccggcc gcgaaagatg
     3061 cgatcgcgca gatcctcacc aacagccgca tgtgcgccga aggcaagcgc ccgatctgcc
     3121 aggacaccgg catcgtcacg gtgttcgtga aggtcggcat ggacgtgcgg tgggatggcg
     3181 cgaccatggg cgtgaccgag atgatcaacg aaggcgtgcg ccgcggttat ctgaacccgg
     3241 acaacgtgct gcgcgcgtcg atcgtgagcc cgccggaagg cgcgcgcaag aacacgacag
     3301 acaacacgcc ggccgtgatc cactacgaga tcgtgccggg cgacaaggtc gacgtgcaga
     3361 tcgcggccaa gggcggcggt tcggagaaca aatcgaagtt ggcgatgctg aatccgtcgg
     3421 actcgatcgt cgactggatt ctgaagaccg tgccgaccat gggcgcaggc tggtgcccgc
     3481 cgggcatgct cggcatcggc atcggcggca cggctgagaa agcgatggtg atggcgaaag
     3541 aatcgctcat ggagccgatc gacatccagg acgtgatcgc acgcggcccg caagactgga
     3601 tcgaagaact gcgcgtggag ctgcacgaga aggtcaacgc gctcggcatc ggcgcgcaag
     3661 gtctcggcgg tctcgccacc gtgctcgacg tgaagatcat ggcagcgccc acgcacgccg
     3721 cgagcaagcc ggtcgcgatc atcccgaact gcgcggccac gcgtcacgcg cacttcacgc
     3781 tggacggctc gggcgtggca aagctcgagg caccctcgct cgacgcatgg ccgaaggtgc
     3841 aatgggaacc gaatacggaa accagcaagc gcgtcgatct gaacacgctg acgccggaag
     3901 aagttgcggg tt
//
LOCUS       DATASH010000897         3518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3909248,
            whole genome shotgun sequence.
ACCESSION   DATASH010000897 DATASH010000000
VERSION     DATASH010000897.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3909248"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /locus_tag="VHV99_23960"
     CDS             complement(<1..915)
                     /locus_tag="VHV99_23960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX3638474.1"
                     /translation="MKTKAAIAWKAGAPLTIEEVDLEGPRAGEVLIEVKATGICHTDY
                     YTLSGADPEGIFPAILGHEGAGVIVDVGPGVGTLKKGDHVIPLYTPECRQCKFCLSRK
                     TNLCQAIRSTQGKGLMPDATSRFSLDGKPLFHYMGTSTFSNYIVVPEIAVAKVREDAP
                     FDKICYIGCGVTTGVGAVVYSAKVEAGANVVVFGLGGIGLNVIQGAKMVGADKIIGVD
                     INPARMELAKKFGMTHFINPNEVENVVDHIVQLTDGGADYSFECIGNTKVMRQALECT
                     HKGWGQSFIIGVAAAGEEISTRPFQLVTG"
     gene            complement(1169..2614)
                     /locus_tag="VHV99_23965"
     CDS             complement(1169..2614)
                     /locus_tag="VHV99_23965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEX3638475.1"
                     /translation="MSFLGIDLGTGSLKAAIIDENGRELAAASVAYALETPHPGWAET
                     SVQTWWRALCAAAARLPAGLRSDVQAIGFSGQMHGVVLIDADGEAVRPAMLWPDTRAL
                     AMLDQWPEPQPNPVAPGMAGPLLRWIVQNEPQSAQMTRWALQPKDWLRVALGGAIATD
                     PSDACATALADPAGVWDQALLERLELPHEWFAPLAPSDAAGGILSRQAAQELGLRAGI
                     VLAIGAADTPCAALGSGLARDGDALLTTGTGGQIVVLAEQAPQAIRGLHRYRAASDHW
                     YRMAAMQNVGVALERVRGWLSYEWADAYRDAFGDATAANSSAATGLTFLPYLTGERTP
                     WLNPAARGGWLGLALDHTRGAMMRAAFEGVAFSLRAGLEAIRASGATVTTLKLAGGGS
                     VDARWRQLLADALNVELHAVDCPNAAPRGAAILGGLASGYWHAGDLATLAPGATRVAG
                     PQGDAALAERYARFLDLYGRVETWFGNAPHP"
     gene            3039..>3518
                     /locus_tag="VHV99_23970"
     CDS             3039..>3518
                     /locus_tag="VHV99_23970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HEX3638476.1"
                     /translation="MQSNTVHPCDERLPAGQLLTLGIQHVLVMYAGAVAVPLIIGAAL
                     KLPKDQIAFLISADLFSCGIATLIQTLGLWIFGIRLPVIMGCTFAAVGPMVAIGTNPS
                     LGILDIFGSTIAAGVIGILLAPAVGKLLRFFPPVVIGVVISVIGLSLMEVGINWAAGG
                     "
BASE COUNT          627 a         1140 c         1087 g          664 t
ORIGIN      
        1 gcccgtcacc agctggaacg gacgcgtgct gatttcctcg cccgccgccg ccacgccgat
       61 gatgaacgac tggccccagc ccttgtgcgt gcactccagc gcctgacgca tcaccttggt
      121 gttgccgatg cattcgaacg agtagtccgc gccgccatcg gtgagttgca cgatgtggtc
      181 gacgacgttt tccacttcgt tcgggttgat gaagtgcgtc atgccgaact tcttagccag
      241 ttccatgcgg gccgggttga tgtcgacacc gatgatcttg tccgcgccaa ccattttcgc
      301 gccttggatc acgttcagcc caatgccgcc aagcccgaac accactacgt tcgcgcccgc
      361 ttccaccttc gccgaataca cgacagcgcc cacgccggtc gtcacgccgc agccgatgta
      421 acagatcttg tcgaacggcg cgtcttcacg caccttcgcg actgcgatct ccggcacgac
      481 gatgtagttc gaaaacgtgg acgtgcccat gtagtgaaac agcggcttgc cgtccagaga
      541 gaaacgcgac gtggcgtcgg gcatcagacc cttgccttgc gtcgaacgga tcgcctgaca
      601 gagattggtt ttgcgcgaca gacagaattt gcactggcgg cattctggcg tgtacagagg
      661 aatgacgtga tcacccttct tcagtgtgcc gacgccagga ccgacgtcga cgatcacgcc
      721 cgcgccttcg tggccgagaa tcgccgggaa gatgccttca ggatccgcgc ccgagagcgt
      781 gtagtagtcg gtgtggcaga tgccggtcgc tttcacttcg atcagaactt cgccggcgcg
      841 cggaccttcg aggtccactt cttcgatcgt caacggggcg cctgctttcc atgcgattgc
      901 tgctttggtt ttcatcgtga atgctcctat gagtctgtga atgcgtggcg cgtcgcgctg
      961 cgatattgcg cggcgcgccg atgacggggt cgatcaatgc ggtccgcctg ctgcgattcg
     1021 gacgcagcgc ttaattggct tgtcgggcag cggacccatt ctgccgctgc cgcagagacc
     1081 aaggtgccga cgcacaacgt agcgcgcgaa agccgtcatg gtattgcaaa gtgtgccgcg
     1141 gcattgttcg tacgtaacat cggttcgcct acggatgcgg agcgttgccg aaccatgttt
     1201 cgacgcggcc atacaaatcg agaaagcgcg cataacgttc ggcaagcgcc gcatcgcctt
     1261 gcggcccggc aacgcgcgtc gcgcccggcg caagcgtggc gagatcgccc gcgtgccaat
     1321 atccgctcgc caatccgccg agaattgcag caccgcgtgg cgccgcgttc ggacaatcga
     1381 ccgcgtggag ctcaacgttc agcgcatcgg cgagcaattg ccgccagcgc gcatcgactg
     1441 agccgccgcc cgcgagcttc aatgtcgtca ccgtcgcgcc gctcgcgcgg atcgcctcaa
     1501 gcccggcacg cagcgagaac gcgacgccct cgaatgcggc gcgcatcatg gcgccgcgcg
     1561 tgtgatcgag cgccaggccg agccagccgc cacgcgcggc cgggttgagc caaggggtgc
     1621 gttcgccggt gaggtacggc aggaaggtga gccctgtcgc agcgctggag ttagcggctg
     1681 ttgcgtcgcc gaacgcatca cgataggcat cggcccattc atacgacagc cagccgcgta
     1741 cgcgttcaag cgcgacgccg acgttttgca tcgccgccat ccgataccag tgatcgctcg
     1801 ccgctcgata gcgatgcagg cctcttatcg cttgcggcgc ctgttcggcg agcacgacga
     1861 tttgtccgcc ggtgcccgtg gtgagcaacg catcgccatc gcgcgcgaga ccgctgccga
     1921 gtgccgcgca tggcgtatcc gcagcgccga tcgccagcac gatgccggcg cgcagaccca
     1981 gttcctgcgc ggcttgccgc gacaaaatgc cacccgccgc atcggacggc gcgagcggtg
     2041 caaaccattc gtgcggtaat tccagccgct cgagcagcgc ttgatcccac acaccggcgg
     2101 gatcggcaag cgcggtcgcg caggcgtcgg aaggatcggt tgcgatcgcg ccgcccagcg
     2161 cgacgcgcag ccaatctttc ggttgcagcg cccagcgcgt catctgcgcg gactgcggtt
     2221 cgttctgcac gatccaacgt aacaaaggac cggccatgcc cggcgcaacg ggattcggct
     2281 gcggctcggg ccactgatcg agcatggcca aggcacgcgt gtcgggccag agcatcgcgg
     2341 ggcgcacagc ttcgccatcg gcgtcgatca gcacgacacc gtgcatctgc ccggagaagc
     2401 cgatggcctg cacgtcgctt cgcaatccgg caggtaagcg cgccgccgct gcgcatagcg
     2461 cgcgccacca ggtttgcacc gacgtttccg cccaaccggg atgcggcgtt tcgagtgcat
     2521 aagcgacact cgccgcggcc agctcgcggc cgttttcgtc aatgattgct gctttgaggg
     2581 agccggtgcc gaggtcgatg ccaagaaaac tcatgggcga tagaacgtca atttgaggcg
     2641 ggtgataagc acgcaaaagg tgcccggaac ggcgtgcctt ccatcaccgc tgcatcactt
     2701 tggaagcgcg cccgaatgcc cacggaaaac ccggaatcgc gggttaaccc cgccgcgcca
     2761 cgagctgcat gaaaacagca ctatgcggcg agcgagatac acctcattga ttctcacaca
     2821 tatcccgtta aggcgggcgc accctgctca tggaccgcac cgccgctgcg cgggacttac
     2881 cgccgatttg tgcggtgcgc ggatttcttt tggctttcaa atgggcctca tgcatatcgt
     2941 caccgggaat gccggaatag gccccagtgg tcttgttgca ctttttcgtt ccgcatacct
     3001 tggagtcatc ccaaaacgag atggtggaga gagacaaaat gcaatcgaac acggttcacc
     3061 cgtgcgacga gcgactgccc gcaggccagt tgctcacgct cggcatccag cacgttttgg
     3121 tgatgtacgc aggcgcggtt gcggtgccgc tgatcatcgg cgcggcgctc aaattgccga
     3181 aagaccagat cgcgtttctg atcagcgccg atctgttttc ctgtggcatc gccacgctga
     3241 tccagaccct cggtctgtgg atcttcggca tccgtctgcc ggtcatcatg ggctgcacct
     3301 tcgcggccgt cggtccgatg gtcgcgatcg gcaccaatcc atcgctcggc attctcgata
     3361 tcttcggctc gacgatagcg gccggggtga tcggcattct tctcgcgcca gcggtcggca
     3421 agttactgcg ctttttcccg ccggtggtga tcggcgtggt gatctcggtg atcggcctgt
     3481 cgctgatgga agtgggcatc aactgggcgg ccggcggt
//
LOCUS       DATASH010000898         2934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5293895,
            whole genome shotgun sequence.
ACCESSION   DATASH010000898 DATASH010000000
VERSION     DATASH010000898.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5293895"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(226..1014)
                     /gene="istB"
                     /locus_tag="VHV99_23975"
     CDS             complement(226..1014)
                     /gene="istB"
                     /locus_tag="VHV99_23975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEX3638477.1"
                     /translation="MNPIPELTSSLKQLRLSGILDTLEARNREAIDRKLAYTDFLTLL
                     IQDEIARRDHRKLDARMRRANFRGQKTLEGFDFDRLPNLNRAVVHDLATCRFIDEKVS
                     VLIAGPCGTGKSHLAQALGHAAARQGHDVLFMTQTRLLGALRNAQAVGSYERRFQALA
                     KVPLLIIDDFGLKPMRTPEDEDFHDLIAERYERTSTVLTSNLDFTEWGDAFATNKMLG
                     AATLDRLRHAAYRIVLDGESYRVPRPMPESNKSALAKGGKNKDS"
     gene            complement(1017..2543)
                     /gene="istA"
                     /locus_tag="VHV99_23980"
     CDS             complement(1017..2543)
                     /gene="istA"
                     /locus_tag="VHV99_23980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011235976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEX3638478.1"
                     /translation="MYQYRQVLVRMRQGDSDRDIDRSKIMGRKKLAAVREIATERGWL
                     SPGNALPDDAQLATAFSRKEFLPASCISSAEPWREQIAQWHDAGVPGTTIHSALVRNH
                     GYEGSYSSVYRLLQQLKAELTPHVPMRLEFKPADAAQVDFGAGPTITDAHTGEIHKTW
                     FFVMTLCWSRHQYVEFVRDQSVDTWLLCHRHAFEWFNGVVARVIIDNPKCAIVKACIY
                     EPEVQRAYAQCAEGYSFKVDPCPPRDPAKKGIVESGVKYVKSSFLPLREFRDLDDANR
                     QAQAWVMHEASTRTHGTTREVPLKRFEDVEQALLLPLPDVPPELATWARVKVHRDAHV
                     QFQRAYYSVPFRLAGKDLWLKATATMVQLYYEHELSASHVRQTAPGTRSTVADHMPPA
                     AQAWQLHDTQWCLREAQRIGPSCYALVHALFGDQVLIKLRAVQGVLRFAPQFGASRLE
                     AACRRANHFGTPSYKAVKLILQKGLDQSWLSTEPVESGATTYTHGGRFCRDTQTLLIH
                     "
     gene            complement(2656..>2934)
                     /locus_tag="VHV99_23985"
     CDS             complement(2656..>2934)
                     /locus_tag="VHV99_23985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638479.1"
                     /translation="VFGKRAPAVTRPIEQKESIKWLEGIDHLAALKVRCAQTRFVCVG
                     DRESDLYELFSAERCELSPLRSQTVFGIKAPGTFRRLGATSLSAYRRQ"
BASE COUNT          563 a          854 c          911 g          606 t
ORIGIN      
        1 gggcattgtg gggccaagct tagcgtgaac tatccaggcc cgaccagcat ccgctatcaa
       61 tgcaccacac atatcctcag ccgtgaccac gcctgctgtg tcatgttcgg cggcctcggc
      121 gcggatcaac tggtggccga gtgttgcctt tcgccgtaaa ggagccgagt gttttcggcg
      181 taatggcgcc agcgaaaggc gcctgattcg gggctcgaac ggggatcaag aatccttgtt
      241 tttacctcct ttcgcaagtg ctgatttgtt gctctcgggc atcgggcgtg gaactcggta
      301 gctctcgccg tcgagcacga tgcggtacgc ggcatgtcgc aggcgatcaa gcgttgcggc
      361 gccgagcatc ttgttggtcg cgaacgcatc accccattcc gtaaaatcaa gattgctggt
      421 caagacggtc gatgtacgtt cgtaccgttc ggcgatcaga tcgtggaagt cttcatcttc
      481 gggtgtgcgc atgggtttca gtccgaaatc gtcaatgatg agcagtggca ccttggcaag
      541 cgcctggaag cgccgttcgt aggagccgac agcctgtgcg ttgcgcagcg cgccgagaag
      601 ccgggtctgg gtcatgaaca ggacatcgtg accttgccgg gcggccgcat ggcccagtgc
      661 ctgcgccaga tgggacttgc ccgttccaca tggaccggcg atcagcacgc tgaccttctc
      721 atcgataaag cggcaggtcg ccagatcatg aacgacagca cgattgagat tcggcaggcg
      781 gtcgaagtcg aaaccttcca gtgtcttctg gccacggaag ttcgcgcggc gcatgcgggc
      841 gtcgagcttg cgatgatcgc gccgtgcgat ctcgtcctga atgagcaacg tcaggaaatc
      901 ggtataggcg agcttacggt cgatggcttc acggttgcgt gcttcgagcg tgtccaggat
      961 gccggacagt cgcaattgct tgagggaaga cgtgagttcg ggaattggat tcatgttcaa
     1021 tggatcagga gggtctgggt atcgcggcag aagcggccgc cgtgggtgta ggtggtcgcg
     1081 cccgattcaa cgggttcggt cgacagccag ctctggtcga gacctttctg gaggatcagc
     1141 ttgacggctt tgtagcttgg cgtgccgaag tgattcgcgc gacggcaggc cgcctccagg
     1201 cgagacgcac cgaactgcgg ggcgaagcgc agcacgccct gtacggcgcg cagcttgatg
     1261 agaacctgat cgccgaagag cgcgtgaaca agcgcgtagc aggacggccc gatacgttgc
     1321 gcttctctca ggcaccattg ggtgtcgtgc agttgccagg cctgtgctgc tggcggcatg
     1381 tgatcggcca ccgtgctacg cgttcctggc gcagtctggc ggacatgcga tgccgacagt
     1441 tcgtgctcgt agtagagctg caccatggtg gcggttgcct tgagccacag atctttaccg
     1501 gcaagccgga acggcacgga atagtaggca cgctggaact gcacatgggc gtcgcgatgg
     1561 accttcacgc gcgcccaggt tgccagctcg ggcggcacat cgggaagcgg cagcaacagc
     1621 gcctgttcga cgtcttcgaa acgcttgagc ggcacctcgc gcgtcgtgcc atgcgtccgg
     1681 gtgctggcct cgtgcatcac ccaggcttgc gcttgccggt tggcgtcatc aagatcacgg
     1741 aattcgcgaa gaggaaggaa gctcgacttc acgtatttga cgcccgattc gacaattcct
     1801 tttttagccg ggtccctcgg cggacacgga tcgaccttga acgaataccc ttcggcgcat
     1861 tgcgcgtagg cccgctggac ttcgggttcg tagatacagg ccttgacgat ggcacacttg
     1921 ggattgtcga tgatgacgcg tgcgaccacg ccgttgaacc actcgaaggc atggcgatga
     1981 cacagtaacc acgtgtcgac actctggtca cggacaaatt caacgtactg atgccgtgac
     2041 cagcacagcg tcatcacgaa gaaccacgtc ttatggatct cgccggtgtg cgcatcggtg
     2101 atggtcggac ctgcgccgaa atcaacctgt gcggcatcag caggtttgaa ctctagccgc
     2161 atcggtacat gcggcgtgag ctccgccttc aactgctgaa gcagcctgta tacggatgag
     2221 tagcttccct cgtagccgtg gttgcgcacc agcgcgctgt gaattgtcgt gccggggacg
     2281 cccgcgtcgt gccactgggc aatctgttcg cgccacggtt cggccgacga gatacagctc
     2341 gctggcagaa actccttgcg actaaacgct gtagcgagct gcgcgtcatc cggcagagcg
     2401 ttgcccggag aaagccaccc gcgttcggtt gcgatctccc gcacggctgc cagcttctta
     2461 cggcccatga tcttcgaccg gtcgatatcc cggtcggagt caccctggcg catgcgcacc
     2521 agaacctgtc gatattggta catctcgaac ttcctcttca agccaccctc cggagcaaat
     2581 gcgtgccggg agggtagcgg ttggcgaagt tcgagcgccc gactcagacg ccagtggcgc
     2641 ctttacggcg aaaatctact ggcgcctata cgccgaaaga cttgtggcgc ctaaacgccg
     2701 aaaagtccct ggcgccttta tgccgaaaac ggtttggctc cttaacggcg aaagttcaca
     2761 gcgttcggcg gaaaacagtt catacaggtc gctctcgcgg tctccaacac agacaaaccg
     2821 cgtctgtgca caacgtacct tgagtgccgc gagatggtcg attccttcaa gccatttgat
     2881 gctttccttc tgttcgatcg ggcgcgtaac cgcgggcgcc ctcttgccga acac
//
LOCUS       DATASH010000899         2825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_7075069,
            whole genome shotgun sequence.
ACCESSION   DATASH010000899 DATASH010000000
VERSION     DATASH010000899.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7075069"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..936
                     /locus_tag="VHV99_23990"
     CDS             34..936
                     /locus_tag="VHV99_23990"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006998898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEX3638480.1"
                     /translation="MDRDRLMSLAARPVRDWPIEIVDETGSTNADLMAQLKEQPRNKT
                     SASAPDSFSPRSTLKAADGTPFVRVAYSQTSGRGRRGRAWVAQPGNALLFSTACVLPR
                     PLEGLAGLSLAVGTAVLDALNSLPLAASARLALKWPNDILLDDGKLAGILIETAWNTP
                     RASAVVIGVGINLHGAAELAAELDEAQRRNALPVPGNTPASLSRAWPEANLTDTLAAV
                     LNALDATLARFAADGFRPFRQQWLAAHAYAGREVVLLEQGVEVARGLAVGIDDNGQLQ
                     IDTPTGLRTATTGDISLRLAEQRP"
     gene            933..1739
                     /locus_tag="VHV99_23995"
     CDS             933..1739
                     /locus_tag="VHV99_23995"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006998897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEX3638481.1"
                     /translation="MSGAPFLLIDAGNSRIKWSLVSAANETLNGGAFEHIDVEALLAD
                     ITDWSSLPKPAEAWISNVAGPRVGEWIAALLDARWPRIPRTIVRARAQQSGVTNRYTE
                     PAALGSDRWCGMIGAHAAYPGENLLIATVGTATTLEALLADGTFTGGLIAPGWLLMMQ
                     SLGTHTAQLPTLPPDTARRAIGSSSDIFATDTRTALSSGCLLAQAGLIERAWQDLRAQ
                     WNNDVRLILSGGGASEVAGALKVPHTRHDSLVLAGLALIAREAAVSRSLD"
     gene            1753..1992
                     /locus_tag="VHV99_24000"
     CDS             1753..1992
                     /locus_tag="VHV99_24000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013337869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638482.1"
                     /translation="MLRWLLALLFLANLLMFVVVQGVFGPLPSAGGREPQHLDRQVHP
                     ELVRVRPISPEEAVDQAVVGGPAPAAAVQAAPLTQ"
     gene            complement(2015..2506)
                     /gene="rfaE2"
                     /locus_tag="VHV99_24005"
     CDS             complement(2015..2506)
                     /gene="rfaE2"
                     /locus_tag="VHV99_24005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014895904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HEX3638483.1"
                     /translation="MPAPFERKIMTRDALAAVRPQFASPVVFTNGVFDILHRGHVTYL
                     ADAKALGATLIVGVNSDASVRMLGKGDDRPINREDDRMALLAALESVDWVVKFEESTP
                     LELIGELHPDVLVKGGDYDMDKLAESALVRSWGGKALAIPFEHDRSTTALLKKVRTQG
                     TEK"
     gene            complement(2680..>2825)
                     /locus_tag="VHV99_24010"
     CDS             complement(2680..>2825)
                     /locus_tag="VHV99_24010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX3638484.1"
                     /translation="KKVVERILHNLSILFERSFATAQELNRYRKEVVLRLQAAQSATQ
                     LPA"
BASE COUNT          488 a          937 c          893 g          507 t
ORIGIN      
        1 aactcgtctt tcccgctcga cgccgcctgg cggatcgacc gcgaccggct gatgtcgctc
       61 gccgcgcggc cggtgcgcga ctggccgatc gagatcgtcg atgaaaccgg ctccaccaat
      121 gccgacctga tggcgcagct caaggagcag cctcgcaaca agacatcagc gtcggcgccc
      181 gactcgttct cgccacgttc gacgctgaag gccgccgatg gcacgccgtt cgtgcgggtt
      241 gcgtactcgc agacatccgg ccggggccgc cggggccggg cgtgggttgc gcaaccgggc
      301 aacgcgttgt tgttttccac cgcctgcgtg ctgccccggc cgctcgaagg gctcgcgggc
      361 ctgagcctcg cggtcggcac agccgtgctc gacgcgctga acagcttgcc gctggcggca
      421 tcggcgagac tcgcgctgaa atggccgaac gacatcctgc tcgacgacgg caagctcgcc
      481 ggcatcctga tcgaaaccgc gtggaacacg ccgcgggcga gcgcggtggt gattggtgtc
      541 ggcatcaacc tgcacggcgc agcagaattg gcggccgagc tcgatgaagc gcagcgtcga
      601 aacgcccttc ccgtgccggg caatacgccg gcgtccctgt cgcgggcgtg gcccgaagcg
      661 aatctcaccg ataccctcgc tgccgtcctg aacgcactcg atgccaccct cgcgcgtttt
      721 gccgccgatg gcttccgtcc gttccgccag cagtggctcg ccgcgcacgc ctacgcggga
      781 cgcgaggtcg tgctgctcga acaaggcgtg gaagtggcgc gcgggctggc ggtgggtatc
      841 gacgataacg gccagttgca gatcgatacg ccgaccggcc tcaggaccgc caccaccggc
      901 gacatctcgc tgcggctcgc ggagcagcgg ccgtgagtgg tgcaccgttt ctacttatcg
      961 atgcaggcaa cagccggatc aagtggtcgc tggtgagcgc cgccaatgaa acgctgaacg
     1021 gcggcgcgtt tgagcacatc gacgttgaag cccttctggc ggatataacc gattggtcca
     1081 gcttgccgaa gcccgccgaa gcatggattt ctaatgtggc gggaccgcgc gtgggcgagt
     1141 ggattgccgc gctgctcgat gcacgctggc ctcgcatacc gcgcacgatc gtgcgcgcgc
     1201 gggcgcaaca atcgggcgtg acgaaccgct acacggaacc agcggcgctc ggcagcgacc
     1261 gatggtgcgg catgatcggc gcgcacgcgg cgtatcccgg cgagaacctg ttgatcgcga
     1321 ctgtcgggac ggcgacgacg cttgaagcgc tgctcgccga tggcacgttc acaggcggcc
     1381 tgattgcgcc cggctggctg ctgatgatgc aatcactcgg cacccacacg gcacaattgc
     1441 ccacgcttcc gcccgacacc gcccgccgcg cgatcggttc atccagcgat attttcgcta
     1501 ccgacacgcg tacggcgttg tcatcaggat gcctgctcgc gcaagctggc ttgatagaac
     1561 gcgcgtggca ggatctgcgc gcccaatgga acaacgacgt ccgcctgatt ctcagcggcg
     1621 gtggcgccag cgaagtggcg ggcgcgctca aggtgccgca tactcgccac gattccctcg
     1681 tgctcgcggg gcttgcattg atcgctcgcg aagccgccgt ttcccgttca cttgattgat
     1741 ggagcgcttt ccatgctgcg ctggctgctc gccctattgt ttctggcaaa cctgttgatg
     1801 ttcgttgtcg tgcaaggtgt gttcggaccg ctgccgtcgg ccggtggacg cgaaccgcag
     1861 catctcgacc ggcaagtaca tccggaactg gtacgggttc gcccgatcag ccccgaagaa
     1921 gccgttgacc aggccgtggt cggcggtccg gcgcccgcgg cggccgtgca ggctgcgccg
     1981 ctcacgcaat aagctctgcc tcgcggcgac gcgcttattt ctctgtgccc tgcgtgcgca
     2041 ctttcttgag caacgcggtc gtcgagcggt cgtgctcgaa tggaatggcc agcgccttgc
     2101 cgccccagct ccgcaccagc gccgattccg ccagcttgtc catatcgtaa tcgccgcctt
     2161 tcaccaacac gtccggatgc agttcaccga tgagttccag cggcgtcgac tcctcgaatt
     2221 tcacaaccca atccacactt tccagcgcgg ccagcagcgc catgcggtcg tcttcgcgat
     2281 tgatcggccg gtcatcgccc ttgccgagca tgcgcaccga cgcgtcactg ttcacaccga
     2341 caatcagcgt tgcgccgagc gccttcgcat cggcgagata ggtcacatgg ccgcgatgca
     2401 ggatatcgaa gacgccattc gtgaaaacca ccggcgacgc gaattgcggg cgcacagcag
     2461 cgagcgcgtc gcgcgtcatg atcttgcgtt cgaaaggagc gggcatgtct gtcaacaaat
     2521 gagggattca gaaggaccgt agtgtgccac gcgtgccggc gcgcgtttaa cttgcggccg
     2581 aacacaagcg tgtttgcgca tggagcgcac ccttcataaa caaacagccc gccggtcaca
     2641 aaccggcggg ctgttcagaa caacaagtcg aaattccggt caggccggca gttgtgtcgc
     2701 gctttgtgca gcctgcagtc gcaacaccac ttccttgcgg taccggttca actcctgcgc
     2761 cgtggcgaac gagcgctcga acagaatgga aaggttatgc aggatccgtt cgacgacttt
     2821 ctttt
//
LOCUS       DATASH010000900         2928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3512798,
            whole genome shotgun sequence.
ACCESSION   DATASH010000900 DATASH010000000
VERSION     DATASH010000900.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3512798"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="VHV99_24015"
     CDS             <1..664
                     /locus_tag="VHV99_24015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX3638485.1"
                     /translation="GNMNLVRATPEFLQALRRLCTEYGSVLIFDEVMCGFRVALGGAQ
                     EVYGITPDLTCLGKVIGGGMPAAAFGGRRDIMAHLAPLGGVYQAGTLSGNPIAVAAGL
                     KTLQLIQAPGFYETLSARTTRLAQGLATVAREAKVPFAADSLGGMFGLYFTDSVPTSF
                     AEVTKSDVPRFNAFFHKMLDAGVYFAPSAYEAGFVSIAHDDTIIDATIDAARGAFASL
                     AA"
     gene            735..1904
                     /gene="ribD"
                     /locus_tag="VHV99_24020"
     CDS             735..1904
                     /gene="ribD"
                     /locus_tag="VHV99_24020"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEX3638486.1"
                     /translation="MFSQTDFVHMERALALAKRGMYTTDPNPRVGCVLVKHGEVIGEG
                     FTQPAGQDHAEVRALKDARSRGHDLRGATAYVTLEPCSHFGRTPPCVNALIEAQVERV
                     VAAMEDPNPQVSGRGLKMLRDAGIEVRCGLLATEAHELNIGFVSRMTRDRPWVRMKVA
                     ASLDGRTGLPSGVSQWITGEAARADGHAWRARASAILTGIGTVRGDDPRMTVRAVDTP
                     RQPQRVLIDAQLDVPPEAQILAGAPTLIFCGNLDERHTERAAALRDRGAEIVQLANAA
                     GKVDLPAMLKVLGERQVNELHVEAGYKLNGSLLREGCVDELLIYLAPSLLGMDSMSMF
                     NLNAPETLDGRVQLNFHAVDRIGDDLRILARFVPRAVPDPAPETGTEPAPQPTSN"
     gene            1920..2549
                     /locus_tag="VHV99_24025"
     CDS             1920..2549
                     /locus_tag="VHV99_24025"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEX3638487.1"
                     /translation="MFTGIVAAVGRIESVKPLGTDGDAGVRLNVEAGGLDLDDVQLGD
                     SITIQGACMTVVEKTAHSFEVDVSRESLNCTVGLGAPGEVNLEKALRAHDRLGGHIVS
                     GHVDGLGTVTRFAPVGESHELRVLAPREIGRYLAYKGSITVNGVSLTVNSVEDRDDGC
                     EFSINLIPHTVQVTSLKNLREGSQVNLEIDLIARYVERMLSTSQDGHTR"
     gene            complement(2643..>2928)
                     /locus_tag="VHV99_24030"
     CDS             complement(2643..>2928)
                     /locus_tag="VHV99_24030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638488.1"
                     /translation="FHSNYFNGCWLDASRSPLNGKVTLSAPPILVTNFLVNHLADFRR
                     ANPGIQLSISGDIRPVSLSRSDLRRSPPIRAVMDFLLRIVAENPAFAKSV"
BASE COUNT          544 a          930 c          954 g          500 t
ORIGIN      
        1 gggcaacatg aacctcgtgc gcgccacgcc ggaattcctg caagccttgc ggcgcctgtg
       61 caccgagtac ggctcggtac tgattttcga cgaagtcatg tgcggtttcc gcgtcgccct
      121 gggtggcgcg caagaggtct acggcatcac gccggatctg acgtgtctcg gcaaagtgat
      181 cggcggcggc atgccggcgg cggccttcgg aggccggcgc gacatcatgg ctcacctggc
      241 gccgctcggc ggcgtctatc aggcgggcac gctgtcgggc aatccgatcg cggtcgccgc
      301 gggcctgaag acgctgcaac tgattcaggc gccgggcttc tatgaaacgc tcagcgcccg
      361 caccacgcgt ctcgcgcaag gtctcgcgac ggtcgcgcgc gaagcgaaag tgccgttcgc
      421 ggccgattcg ctcggcggca tgttcggcct ctacttcacc gattcggtac caacgagttt
      481 cgccgaagtc acgaagagcg acgtaccgcg cttcaacgcg ttcttccaca agatgctcga
      541 cgcgggcgtg tacttcgcgc cgtcggcata tgaagcgggc ttcgtttcga tcgcgcacga
      601 cgacacgatc atcgacgcga cgatcgacgc agcacgcggc gcatttgcct cgctcgcggc
      661 ctgaaacggg cgagacaagg aacccgccga taaccaagcc aattctgact agccactgca
      721 ccgaccggat accgatgttt tcgcaaaccg acttcgtcca tatggaacgc gcgctcgcgc
      781 tggccaagcg cggcatgtac acgaccgatc cgaatccgcg tgtcggttgc gtgctcgtca
      841 agcacggcga ggtgatcggc gaaggcttca cgcaaccggc cgggcaggac cacgccgaag
      901 tccgcgcgct gaaggacgcg cgctcgcgcg gccatgatct gcgcggcgcc acggcctatg
      961 tgacgctcga gccgtgcagc catttcggcc gcacgccacc ctgcgtgaat gcgctgatcg
     1021 aagcacaggt cgagcgcgtg gtcgcggcga tggaagatcc caatccacaa gtttccgggc
     1081 gcggtctcaa gatgctgcgc gacgccggta tcgaagtgcg ttgcgggctg ctcgccaccg
     1141 aagcgcacga actgaatatc ggcttcgtgt cgcgcatgac gcgggaccgt ccgtgggtcc
     1201 gcatgaaggt ggcggcctcg ctggacggcc gcaccggctt gccgtcgggt gtcagtcaat
     1261 ggattactgg cgaagcggct cgcgccgacg gtcatgcgtg gcgcgcccgc gcgtcggcga
     1321 ttctgacggg catcggcacc gtgcggggag acgatccgcg catgaccgtg cgagccgtcg
     1381 acacaccgcg ccaaccgcaa cgcgtgctga tcgacgccca gctcgacgtg ccgcccgaag
     1441 cgcagattct ggccggcgca cccaccctga ttttctgcgg caatctcgac gagcgtcata
     1501 ccgagcgcgc cgccgcgctg cgcgatcgcg gcgcggagat cgtgcaactg gcaaacgcgg
     1561 cgggcaaggt cgacttgccc gccatgctga aagtgctcgg cgagcgtcag gtgaacgaac
     1621 tgcacgtcga ggccggctac aagctgaacg gctcgctgct gcgcgaaggc tgcgtcgacg
     1681 agctgctgat ctatctggcg ccgagcctgc tcggcatgga ctcgatgagc atgttcaatc
     1741 tgaatgcacc ggaaacgctc gacggccgcg tgcaactgaa tttccacgcg gtcgaccgga
     1801 tcggcgacga tctgcggatt cttgctcgct tcgtgccccg cgccgtgccg gatccggcac
     1861 ccgaaaccgg caccgagccc gcaccccaac caacttcgaa ttgatcaagg attagcacga
     1921 tgtttacagg aatcgtcgcg gcagtcggcc gcatcgaatc agtcaaaccc ctcggcaccg
     1981 atggggatgc gggcgtgcgc ctgaacgtcg aagccggcgg tctcgatctc gacgacgtgc
     2041 aactcggcga cagcatcacg atccagggcg cctgcatgac ggtggtggaa aaaaccgcgc
     2101 attcgttcga agtggacgtg tcgcgcgaaa gtctgaactg cacggtcggt ctgggcgcgc
     2161 cgggcgaagt caatctcgag aaagcgctgc gcgcgcacga ccggctgggc ggccacatcg
     2221 tctccggtca cgtggacggc ctcggcacag tcacgcgttt cgcgccggtc ggcgagtcgc
     2281 acgaactgcg tgtgctggcg ccgcgcgaga tcggccgcta tctggcctac aaaggctcga
     2341 tcacggtcaa cggcgtgagc ctgacggtga actcggttga ggatcgcgac gacggctgcg
     2401 aattttcgat caacctgatt ccgcacacgg tgcaggtgac gtcgctgaag aacttacgcg
     2461 agggttcgca ggtgaatctg gagatcgatc tgattgcgcg gtatgtggag cggatgctca
     2521 gcacgtctca agacggccat acccgttgaa ataatgaggc acgttcggcg gcgtcgacgc
     2581 ggttcggatc gcgctgcgca tgctgtctca ttgaccgccc gcgcagatgc ctccctccgc
     2641 gtctagacac ttttcgcaaa agccggattc tccgccacga ttctcagcag gaagtccatg
     2701 acagccctga ttggcggcga acggcgcagg tcgcttcggc tgagcgaaac cggccgtatg
     2761 tcgccggaaa tggacagctg tatgccagga ttcgcgcgcc gaaagtcggc gagatggttc
     2821 accaggaaat tcgtgaccag gatgggcggt gcgctcagcg tgaccttgcc gtttagcggc
     2881 gaacggctgg cgtcgagcca gcaaccgtta aagtaattgg aatgaaaa
//
LOCUS       DATASH010000901         1968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3118234,
            whole genome shotgun sequence.
ACCESSION   DATASH010000901 DATASH010000000
VERSION     DATASH010000901.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3118234"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..913)
                     /locus_tag="VHV99_24035"
     CDS             complement(23..913)
                     /locus_tag="VHV99_24035"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016354688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX3638489.1"
                     /translation="MTSIGATGLKLSAGHDNPEQALAALAKVELVISDCDGTLLYTDK
                     SLGEPAKQAVRNLNAAGVRFTVASSRPGKGMHSIVETLGIQSPFSAFNGGKLVDPTGW
                     KTLSAHWLSRDAAQTALESLTRDGVDAWVFIDDQWYITNPGGEYVPLERRTVGYDAVV
                     VKRFEDIDLDSVEKIVASTKDAALLQRKEAEFQDTLKGRAMAMRSQAYYLDITHPLAN
                     KGEGVRELARLAGVALENVAVLGDMGNDVAMFDVAGVSIVMGQASPDVQSHATFVSAT
                     NDEDGFAAGLNGLLAARKGN"
     gene            complement(910..1761)
                     /locus_tag="VHV99_24040"
     CDS             complement(910..1761)
                     /locus_tag="VHV99_24040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX3638490.1"
                     /translation="MGTAARKAAVKKTSAVKKAAGKAAADKTARRSSAKERILAIDVG
                     GTGLKAAIIDAHGKMQTERVKVATPHPCPPDVLVQKLVELVGPLLTPLPATHISIGFP
                     GVVRDNHVLTAPNLGNEFWRNAPLADMLAEHLGHLPVRIINDAEMQGLAAIEGKGIEF
                     VLTLGTGAGTALFRDGELMPHLELAHHPVNKNKTYDEYLGNAARDKAGNKRWNRRVEK
                     IIGILSSLVNYDRLWIGGGNAANLNFELPDNVKVVSNEAGIEGGARLWHPRSVRETRQ
                     LPAGAFE"
     gene            complement(1772..>1968)
                     /locus_tag="VHV99_24045"
     CDS             complement(1772..>1968)
                     /locus_tag="VHV99_24045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX3638491.1"
                     /translation="SIETSWAAVDGVLHPEGGPLPVHGYTAGSAGPKEADDLLKADGR
                     AWRPLVADDHTQKTDHDDKN"
BASE COUNT          320 a          670 c          564 g          414 t
ORIGIN      
        1 cacgcgtgtc cagccaccaa cttcaatttc ccttccgggc cgccagcaaa ccgttgaggc
       61 ctgcggcaaa gccgtcttca tcgttcgtag ccgacacaaa cgtcgcgtgc gattgaacgt
      121 ctggcgacgc ctgccccatc acgatggaaa cacccgccac atcgaacatg gcgacgtcgt
      181 tgcccatgtc ccccagcacg gccacgtttt ccagcgcaac gcccgccagc cgcgccagtt
      241 cgcgtacgcc ctcgcctttg ttcgcgagcg gatgcgtgat gtcgaggtaa taggcttgtg
      301 agcgcatcgc catggcgcgg cctttgagcg tgtcctggaa ctcggcctcc ttgcgttgaa
      361 gcagcgccgc gtctttcgtc gacgccacga tcttttcgac cgagtccagg tcgatatcct
      421 cgaaacgctt gaccaccacc gcgtcgtatc ccacggttct gcgctctagc ggcacgtatt
      481 cgccgccggg attcgtgatg taccactggt cgtcgatgaa cacccacgcg tccactccgt
      541 cgcgcgtgag gctctccaga gccgtttgcg cggcgtcgcg cgacaaccag tgagcgctca
      601 gggttttcca gccggtgggg tcgacgagtt tgccgccgtt gaaagcggaa aacggcgact
      661 ggattccaag cgtttcgacg atcgaatgca tacccttgcc cggccggctg ctcgccaccg
      721 tgaagcggac acccgcggcg ttgagattgc gcacggcttg cttcgcgggc tcacccagcg
      781 atttgtccgt gtagagcaag gtgccgtcgc aatccgaaat cacgagttcg accttcgcca
      841 acgccgccag cgcctgctcc gggttgtcgt gaccggcact gagcttaaga ccggtagcgc
      901 cgatggatgt cattcaaacg ctccggccgg caattgccgc gtttcgcgca ctgaacgcgg
      961 gtgccagagt cgcgcgccgc cttcaatacc tgcttcgttc gagacgactt tcacgttatc
     1021 cggcaactcg aaattgaggt tggccgcgtt gccgccgcca atccacagac ggtcgtagtt
     1081 cacgagcgaa ctcaggatgc cgatgatctt ctccacgcgg cgattccacc gcttgttgcc
     1141 cgccttgtcg cgcgccgcat tgccgagata ttcatcgtaa gtcttgtttt tgttcaccgg
     1201 atgatgcgcc agctcgagat gcggcatgag ttcgccgtca cggaaaagcg ccgtacccgc
     1261 gcccgtgccc agcgtcagca cgaactcgat gcctttccct tcgatagcag caagcccctg
     1321 catctcggcg tcgttgatga tgcgcaccgg caggtgccca aggtgctcgg ccagcatgtc
     1381 cgcgagcggc gcgttgcgcc agaattcgtt gccaaggttc ggtgcggtca acacgtggtt
     1441 gtcccgcact acgcccggaa aaccaatcga gatatgcgtg gccggcagcg gcgtcaacag
     1501 cgggccgacc agttccacca gcttctgcac caacacgtcc ggcggacacg gatgcggcgt
     1561 ggccaccttt acccgctcgg tttgcatctt cccgtgcgcg tcgataatcg cggccttcag
     1621 gcccgtcccg cccacgtcga tggcgagaat ccgctctttc gcgctcgaac gtctggcggt
     1681 cttgtctgcg gctgccttgc ctgcggcttt cttcactgcc gaggtcttct tcactgcggc
     1741 cttgcgtgct gctgtcccca tacggttctc ctcagttctt gtcgtcgtga tcggtttttt
     1801 gcgtgtgatc gtccgccacc agcgggcgcc aggcgcggcc gtcggccttc aacagatcgt
     1861 cggcttcctt cgggcccgcg ctgcccgcgg tgtagccatg taccggcaac ggcccacctt
     1921 cgggatgcaa cacgccgtca accgccgccc agcttgtttc aatgctgt
//
LOCUS       DATASH010000902         6250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3711272,
            whole genome shotgun sequence.
ACCESSION   DATASH010000902 DATASH010000000
VERSION     DATASH010000902.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6250
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3711272"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1352
                     /locus_tag="VHV99_24050"
     CDS             <1..1352
                     /locus_tag="VHV99_24050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen debranching enzyme GlgX"
                     /protein_id="HEX3638492.1"
                     /translation="GPTLSFRGLDNSSYYRLVPDNPRHCINDTGTGNTLNLSHPRVLQ
                     MVTDSLRYWVTEMKVDGFRFDLATILGRELHGFDEGGGFLDSCRQDPVLSSVRLVAEP
                     WDCGPGGYQVGGFPPGWAEWNDRFRDTVRGYWKGDEGEVPDLAKRLTGSGDKFNRRGR
                     RPWASVNFVAAHDGFTLNDLVSYNDKHNEANGEDNNDGHSDNKSWNMGVEGPSDDPEI
                     RQQRERQKRNLLATLLLSQGTPMMLAGDEFGRTQQGNNNAYCQDNEISWVDWEGIDDD
                     GRALIEFVKNLTTLRHRLPVLRRGRFLSGEYNEALEVTDARWLAPDGTDLSQEQWDDP
                     SMRCFGLVIDGRAQASGIRRPASDATLLLVLNAHHDVVNFTLPDIPEGDQWTCLLDTN
                     MPVRSELPQFSAGDAYQVTGRSLLLFALEAPSRATQRVFDRLEEQLTTDENDPPAGS"
     gene            1510..1689
                     /locus_tag="VHV99_24055"
     CDS             1510..1689
                     /locus_tag="VHV99_24055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HEX3638493.1"
                     /translation="MEQEQTSREEQIRTRAYYLWEQAGDPKGTPDEYWEQARDEVEKE
                     APPVESGPVAGTPKK"
     gene            1712..3703
                     /locus_tag="VHV99_24060"
     CDS             1712..3703
                     /locus_tag="VHV99_24060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX3638494.1"
                     /translation="MKDFPDPRGARPAARIHAPPAAFGIDGLIAIAVGVTIVACLYFA
                     RAVLIPITLAILLSFLVAPLANALGRLKLGHVASVFAAVVISVSVIGMLGAVIATQLT
                     DLAAGMPRYQATIERKMDAAHSLTIGKLNRLAGAAGQALQRATVEPPQPQPPRDASSP
                     ASVHAPAAVPVEVREPVPTPFELARRVVSPAISPLETAFIVFVVMVVILLQRDDLRDR
                     AIRLFGSRDLHRTTTVMDEAARRLSRYFVSQLGLNAGVGVVIGAGLFFIGVPSPILWG
                     ILAALLRLVPYVGIWISATLATALAAAVSPGWTMAIWSLALFVTIELVVGQVVEPLLY
                     GRSTGLSPFSVVVAAIFWSWIWGPIGLILSTPLTLCLLVLGRHVRRLEFLDVLLGDQP
                     ALTPIENFYQRALAGDPDEAIEQAEALLRERSLSAYYDEVAIKGLQLAANDVTRGSVT
                     ATQLARIEATTNDLVDGLDGYEDSRPAPQPTEDDWSAAGPMAPAAEVEGTPRALRTTS
                     TGATEEGRGSDANAAANRVLCIPGRGPLDPLATTILLQLLGKHGFAARALPHEAASRA
                     SIDSLEADDVGTVCILYLQIDGIPSHLRYLVRRIRAKLPTVSIVVGLWAAQDTEKWSA
                     DLQSAMGADCYASSLQEVLTACRRIEVPQEDPGLAIVNG"
     gene            complement(3868..4050)
                     /locus_tag="VHV99_24065"
     CDS             complement(3868..4050)
                     /locus_tag="VHV99_24065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638495.1"
                     /translation="MPEHKEKHPHANKTEKQIDKEVEDSFPASDPPSTGGTTKIVPDK
                     HAASKNKPSTGKPAGR"
     gene            complement(4120..5670)
                     /locus_tag="VHV99_24070"
     CDS             complement(4120..5670)
                     /locus_tag="VHV99_24070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3638496.1"
                     /translation="MSTSARCVAQLSQLRADRAERIVVDGENILLVRDGDTVRAYAAD
                     CPHAGGPLEKGALCNGRIICPWHKATFDVTTGDVLEPPALVALDRYPVTVTGDDVIVT
                     PEKLPQPLRNASAKSPHFVVIGAGAAGAAACAALRECGFTGHVTLVGEEPHAPYDRTA
                     LSKFVPSGEMTPGDVPPLLAPDWLARHDVERLVAKVARLDVPGRTIHFETGGELAYDT
                     ALLASGSVPKIPSIPGHDLGGVYVLRTLDDAATLVDALGEDAGNAQVVILGSSFIGLE
                     TASSLRKRGTQVTVVSPETIPFAKQFGERAGTMFRELHERNGVVFHLGATVASLEGEE
                     GNVHEVMLESGEHVAADLVLLGTGVAPATGFVEGLPLQKDGGVIVNAGMQAAPHLYAA
                     GDIAVFPLHEDQEPVRIEHWRVAQQHARIAAENMCGARHRYVGVPYFWTYHFGKRFEY
                     LGHASEWDDIVIDGELDRQKFVALYVKEEKVVAVLACEREAQTAQLIDAMGSGLSRAD
                     AMKIVLSD"
     gene            5894..>6250
                     /locus_tag="VHV99_24075"
     CDS             5894..>6250
                     /locus_tag="VHV99_24075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018438050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEX3638497.1"
                     /translation="MEIVTVQRAERIAVDVPMPLRNFAATRRVLLIVLAVSIVFPLAC
                     LAGYGYFDYQRRIADSNDMIDRLARVTEEQAVKVLDLDQQMASRIVDLLGNDDDARIR
                     EQQAGLHDRLRQIGGDF"
BASE COUNT         1082 a         1976 c         2014 g         1178 t
ORIGIN      
        1 tcggcccgac gctctcgttc cgcgggctcg acaattcgag ctattaccgc ctcgtcccgg
       61 acaatccgcg ccactgcatc aacgataccg gcaccggcaa cacgctcaat ctgtcccatc
      121 cgcgcgtgct gcagatggtc accgacagcc tgcgctattg ggtgaccgag atgaaagtcg
      181 acggtttccg tttcgacctc gccacgattc tcggccgcga gctgcacggt ttcgacgaag
      241 gcggcggctt cctcgacagt tgccggcagg acccggtgct ctcctccgtg cggctggtcg
      301 ccgagccgtg ggattgtggc cccggcggct atcaggtggg cggctttccg cccggctggg
      361 ccgagtggaa cgatcgcttt cgcgataccg tgcgcggcta ctggaagggc gatgaaggcg
      421 aggttcccga tctcgccaaa cgcctgaccg gctcgggcga caagttcaat cgtcgcgggc
      481 gccgcccatg ggccagcgtg aacttcgtgg ccgcccacga cggcttcacg ctcaacgatc
      541 tggtctcgta caacgacaag cacaacgagg ccaacggcga agacaacaac gacggccatt
      601 ctgacaacaa gtcatggaac atgggcgtgg aaggcccgag cgacgatccg gaaatccgtc
      661 agcagcgcga gcgccagaag cgcaatctgc tcgccacgtt gttgctctcg caaggcacgc
      721 cgatgatgct cgccggcgac gaattcggcc gcacgcagca gggcaacaac aatgcgtatt
      781 gccaggacaa cgagatcagc tgggtcgatt gggaaggcat cgacgacgac gggcgcgcgc
      841 tgatcgagtt cgtcaagaat ctgaccacgc tgcggcatcg gttgccggtc ctgcggcgtg
      901 gacgctttct cagcggcgaa tacaacgagg cgctcgaggt gaccgacgcg cgttggctcg
      961 cgccggacgg caccgatctt tcgcaggaac agtgggacga tccgtcgatg cgctgcttcg
     1021 gcctcgtgat cgacggacgc gctcaggcga gcggcattcg acgtcccgcc tccgatgcga
     1081 ccttgctgct ggtgctcaac gcgcatcacg acgtggtcaa tttcacgctg cccgatatcc
     1141 ccgagggcga tcagtggacg tgtctgctgg acaccaatat gccggtgcgt tccgagttgc
     1201 cgcaattcag cgcgggcgac gcgtatcagg tgaccggccg ttcgttgctg ctgttcgcac
     1261 tcgaagcacc gagccgcgcg acgcaacgcg tgttcgaccg cctggaggag cagctgacga
     1321 ctgacgaaaa cgaccctccc gccggatcgt gagacggagc ttgaggctgg ccgtaagcgc
     1381 aagccgcgaa gtattgcgcc gggcatagcg tttgctcgat tccgcatagc cgcgcgtctg
     1441 ccgtattccg cgacagacgc gcactgcaac cggtgtcgat ttccgcccgg tccaaccgag
     1501 ggtgaacaaa tggaacagga acaaacgtcc agggaagaac agattcgtac acgcgcctac
     1561 tacctctggg agcaggcggg ggacccgaag ggaacaccgg acgagtattg ggagcaggcc
     1621 cgcgacgagg tcgagaaaga agcgccgccg gtagaaagcg gcccggtggc tggcacgccg
     1681 aagaagtgaa agaggtctga acggacacgc cgtgaaggat ttccccgacc cgcgtggcgc
     1741 ccggcccgcc gcccgcattc acgcaccgcc tgccgcattc ggcattgacg gcctgatcgc
     1801 gattgcggtc ggcgtcacca tcgtcgcctg cctgtacttt gcgcgtgccg tgctgattcc
     1861 gatcacgctc gccatcctgc tgagtttcct tgtcgcgccg ctggcgaatg cgctcggacg
     1921 tctgaagctc ggacatgtcg cgtccgtgtt cgccgcggtg gtgatttcgg tttcggtgat
     1981 cggcatgctt ggcgcggtga tcgctaccca gctgaccgac cttgccgcgg gcatgcctcg
     2041 ctatcaggcg accatcgagc gcaagatgga cgccgcgcac agcctgacca tcggcaagct
     2101 gaaccggctc gcgggcgcgg ccggccaggc cttgcaacgc gcgaccgtcg aaccgcccca
     2161 accgcaaccg ccgcgcgatg cctcatcgcc cgcgagtgtt cacgcgcctg cggccgtgcc
     2221 tgtcgaagtg cgcgaaccgg tacccacacc gttcgaactc gcgcggcgcg tcgtgtcgcc
     2281 cgcgatcagt ccgctggaga cggcgtttat cgtattcgtc gtgatggtgg tgatcctgct
     2341 gcaacgcgac gatctgcgcg atcgtgccat acgtctgttc ggttcacgcg acctgcatcg
     2401 cacaaccacc gtgatggacg aagccgcgcg gcgcctgagc cggtacttcg tttcgcaact
     2461 cggcctgaat gcgggcgtcg gggtggtgat cggcgcgggt ttgttcttca tcggcgtgcc
     2521 gagtccgatc ttgtggggga ttctcgccgc gttgttgcgg ctcgtgccgt acgtcggcat
     2581 ctggatttcc gcgacgcttg ccaccgcgct cgccgctgcc gtgagtcctg gctggaccat
     2641 ggcgatctgg tcgctggcgc tgttcgtgac gatcgaactg gtggtggggc aagtggtcga
     2701 gccgttgcta tacggacgaa gcaccgggct ctcgccattt tccgtcgttg tcgcggcgat
     2761 tttctggagc tggatctggg ggcccatcgg gttgattctg tcgacgccct tgaccttgtg
     2821 cctgctggtg ctcggacggc acgtgaggcg cctggagttt ctcgatgttc tgctgggcga
     2881 tcagccggcg ctgacaccga tagagaactt ttaccagcga gcgcttgcgg gcgatcccga
     2941 cgaagcgatc gagcaagcgg aggcactctt gcgcgagcgc tcgctgtcgg cttactacga
     3001 cgaagtggcg atcaagggct tgcaactcgc ggccaacgac gtaacgcgtg gcagcgtgac
     3061 ggcgacccag cttgcgcgga tcgaggcgac cacgaacgac ctcgtggacg gtctggacgg
     3121 ctacgaggac agcagaccgg cacctcaacc aacggaagac gactggagcg cggcggggcc
     3181 gatggcaccg gcagccgaag tggaaggcac cccgcgcgcg ctgcggacca cctcgacggg
     3241 cgctacggaa gaggggcgcg gcagcgatgc gaacgcggcg gcgaatcgcg tgttgtgtat
     3301 tcccggccgc ggtccgctcg atccgctcgc gaccacgatc ctgttgcagt tactcggcaa
     3361 gcacggtttc gcggcgcggg cattgccgca tgaagccgct tcgcgggcgt cgatcgatag
     3421 tctcgaggcg gacgatgtcg ggaccgtttg cattctctac ctgcagatcg atgggattcc
     3481 ttcgcatctg cgctatctgg tccgacgtat tcgcgcgaag ttgccgacgg tgtcgatcgt
     3541 agtggggttg tgggccgcgc aggatacgga gaagtggagt gcggatctgc aaagtgcgat
     3601 gggcgcggat tgttatgcca gctccttgca ggaagtgttg accgcctgcc ggcgcatcga
     3661 ggtgccccag gaggatccgg gactggccat cgtcaatgga tgatccttcc tgcctcttcg
     3721 agcagtgaaa tgcatcaacc gccgttcggc gaatcggatg ctccggaaac acccaatgca
     3781 tccggcgcat cctggcatcc gcctccgtga gtaccttgta aaggccgccc ggcagcctgg
     3841 ccggacggcc tgttcaagtg gaggcgccta gcggcctgcc ggcttgccgg tcgaaggctt
     3901 gtttttcgag gcggcgtgct tgtcggggac gatcttggtc gtaccgcctg tggaaggtgg
     3961 atcgctagcc gggaagctgt cttcgacttc cttgtcgatc tgcttctcag tcttgttggc
     4021 gtgtggatgt ttttccttgt gctctggcat ggcaacttct ccttcatgta agcgcggaaa
     4081 accgcgcggg cagactcttc aatcaccgca tgcctcgcgt caatcgctca gcacgatttt
     4141 catggcatca gcgcgagaca agccgctacc catcgcatcg atcagttgcg cggtttgcgc
     4201 ttcgcgttcg caggcgagca cggcgactac cttctcttcc ttcacataca gcgcgacgaa
     4261 tttctgccga tcgagttcgc cgtcgatcac aatgtcgtcc cactcgctcg catgaccgag
     4321 atactcgaag cgcttgccga agtgataggt ccagaaatac ggcacgccga cgtagcggtg
     4381 acgcgcgccg cacatgtttt cggcggcgat gcgcgcgtgt tgctgcgcga cgcgccaatg
     4441 ttcgatgcgc accggttcct gatcttcatg cagcggaaac acggcgatat cgccggctgc
     4501 gtaaaggtga ggcgcggcct gcatgcccgc gttgacgatc acgccgccat ccttttgcag
     4561 cggtaatcct tcgacgaacc cggtggccgg tgcaacgccc gtacccagca gtacgagatc
     4621 ggcggcgaca tgttcgccac tttcgagcat cacctcatgc acattgcctt cctcgccttc
     4681 gagcgaggcg actgttgctc cgaggtggaa caccacgccg ttgcgctcgt gcagttcgcg
     4741 aaacatggtg cccgcgcgct caccgaactg tttggcgaag gggattgttt ccggcgaaac
     4801 gacagtgact tgcgtgcccc gtttgcgcag cgacgaagcc gtttccagac cgatgaagct
     4861 gctgccgaga atcacgacct gcgcgttccc ggcgtcctcg ccgagcgcat cgacgagcgt
     4921 ggcggcgtcg tcgagcgtgc gcagcacgta cacgccgcca aggtcatgcc cgggaatgga
     4981 tggaatcttc ggcacgctgc cgctggcgag tagcgcggtg tcgtacgcca gttcgccgcc
     5041 ggtttcgaaa tggatcgtgc gcccgggcac atcgagccgc gcgaccttcg cgaccagacg
     5101 ttcgacgtcg tgccgcgcga gccaatccgg cgcaagcaaa ggcggcacat cgccgggcgt
     5161 catctcgccc gacggcacga atttgctgag cgcagtgcgg tcgtagggcg catgcggctc
     5221 ctcgccgact agcgtgacgt ggccagtaaa accgcattca cgtagcgccg cgcacgccgc
     5281 ggcacctgcc gcgcccgcgc cgatcaccac gaaatgcggg cttttcgcgg acgcattgcg
     5341 caacggctgc ggcagtttct ccggtgtgac gatcacgtcg tcgcccgtca ccgtcactgg
     5401 atatcggtcg agcgcgacca gcgcgggcgg ttcgagcacg tcgcccgtgg tcacatcgaa
     5461 agtggctttg tgccacggac agatgatccg gccgttgcac aacgcgcctt tctccaacgg
     5521 tccgccggca tgcggacagt ccgccgcata ggcgcgcacg gtgtcgccgt cgcgcaccag
     5581 cagaatgttt tcgccgtcca ccacgatgcg ttcggcgcgg tccgcccgta gttgcgatag
     5641 ctgcgcgacg catcgggcag acgttgacat ggtcgattcc tcgcggaaga taagtggaca
     5701 ccccgaacgt ccttgcggca gcaaaccgtg tgcctggtca cgcgccgctc cacgtgtgac
     5761 gttttgctcg catgccggcc tagggagaac ggcggaagtg cggtaaattc cgggaaaagg
     5821 aaatagcgca cgaacagcgt ggcgcgtgcg tggggagcag ttgttacgct cacctgacct
     5881 gtatttacct ttcatggaga tcgtgaccgt gcagcgtgcg gagcgtatcg cagtcgatgt
     5941 ccccatgccg ttgcgcaatt tcgcggcgac gcggcgcgtg ctgctgatcg tgcttgccgt
     6001 ttcgatcgtg ttcccccttg cgtgtctcgc gggttatggc tatttcgatt accagcgccg
     6061 catcgccgat tcgaacgaca tgatcgaccg gctcgcgcgc gtcaccgaag aacaggccgt
     6121 caaggtgctg gacctcgacc agcaaatggc ctcgcgtatc gtcgatctgc tcggtaatga
     6181 cgacgacgcg cggattcgcg agcagcaagc cggcctgcac gatcgtctgc ggcaaatcgg
     6241 cggcgacttt
//
LOCUS       DATASH010000903        11750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3513775,
            whole genome shotgun sequence.
ACCESSION   DATASH010000903 DATASH010000000
VERSION     DATASH010000903.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 11750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11750
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3513775"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(410..1396)
                     /locus_tag="VHV99_24080"
     CDS             complement(410..1396)
                     /locus_tag="VHV99_24080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3638498.1"
                     /translation="MKIDSHNLNDLMYFSQVVEHGGFSAAERVLGISKSRLSRRLTEL
                     EASLGVRLLQRSTRKLALTEAGQLFYQHCQAMLSEAQAAVNVVQQLRASPRGTVRVSV
                     PVTISQTILSQILPEFMHRFPEVRVVMRVTNRVVDLFEDSIDIALRVRSDPPENANIV
                     VRPLWRTQQMLVGAPSLLQQNAPPLLPADLSRFETLDVPTSDGRHVFDLIAPDGTRHA
                     HEHEPRLVTADLMTIREAVLSGVGIAALPEMMYGSALRAGQLSPVMPDWTFPTPQLYA
                     VFVSRQGMVPAVRAFVDYLVEMLDPDEGKHIMGECPTHEVRTMAEPVLAFAS"
     gene            1519..2397
                     /locus_tag="VHV99_24085"
     CDS             1519..2397
                     /locus_tag="VHV99_24085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX3638499.1"
                     /translation="MSTTRTIERTFPAVRTTEGGGFIVNRPFPTRLLMDFDPFLLLDE
                     MGPIDYAPGEAKGAPDHPHRGFETVTYVLEGQFGHKDSAGHSGTLHAGDVQWMTAGAG
                     VVHSEMPDPSFVRTGGRVHGLQLWVNLPRRDKMIAPRYQEMPSASIPVATSADGKVRV
                     KVIAGEALGVKAAIETRTPILYQHFSLQPGATIQQPVPADYRVFAYSLSGKGFYGAGE
                     TRQEIGAQTMVVFENDGESVTLAAGEEPLEVLLLGGVPLKEPVVRYGPFVMNTEGEIR
                     QAVIDYQAGRMGAITH"
     gene            2622..3020
                     /locus_tag="VHV99_24090"
     CDS             2622..3020
                     /locus_tag="VHV99_24090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018432407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX3638500.1"
                     /translation="MAESTVTAHIGSTNFQVLFDDGKHTWLADEPESLGGGDRGPTPV
                     ALLLSSLGACTSITLKMYAQRKGWPLADVRVALSLETGDAGSTIDRKIVLEGDLSDEQ
                     RERLLQIANACPVHKILTHSITIRSGLAVA"
     gene            3098..3580
                     /locus_tag="VHV99_24095"
     CDS             3098..3580
                     /locus_tag="VHV99_24095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX3638501.1"
                     /translation="MNFEHLIQINDPLNPLVDSMTREQLWEGLVLRAEQPQLFVIGLD
                     SCTILSREGDTLERELHYGQATVRDRVTLQDRQSVRYDILPTAEYVGGSLTMTIEQPD
                     DLQLFLRFEYATTLPVSDDQDAVQTQEIVKSAYRENDIDTVRLIRQYVQTRQEPGSLH
                     "
     gene            3767..4027
                     /locus_tag="VHV99_24100"
     CDS             3767..4027
                     /locus_tag="VHV99_24100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HEX3638502.1"
                     /translation="MTDLTHASTLSLRRPAAALTSRPKASTATATAAKPAADRANGSG
                     DTSDRVVRSDTLLQGHSHVSIVHNGETYQLRATRLGKLILTK"
     gene            complement(4323..5729)
                     /locus_tag="VHV99_24105"
     CDS             complement(4323..5729)
                     /locus_tag="VHV99_24105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX3638503.1"
                     /translation="MSTVMTRPGRLAAAGQWMQRHGAAIRGIQWVVVAVYAFLILVPA
                     VMPLPDDTAHLWNNLTLAAQFVFWGIWWPFVLLSMVMLGRVWCGVLCPEGALAEFASK
                     YGRGWAIPRWMRWGGWPFVAFGITTIYGQMVSVYQYPKAVLLVLGGSTFAAMIIGLLY
                     GREKRVWCKYLCPVNGVFSLLARLAPFHYKVDEDAWRRSYKNGEHGHRVIPINCAPLV
                     PLRNMKGASDCHMCGRCSGHRDAIALTWRAPSSEVVQLGDRQANPWDTALILYGLLGI
                     AIGAFHWTASRWFVDLKMFFATWLVDHDITWPLDTNAPWFLFTHYPEQNDVFTWLDGT
                     MVIGYILATALVYGTVLLVLLMGATRMLGRFSSVRLHHLTQGLIPIAGAGVFLGLSAT
                     TLSLLRAEHVSLWWASDMRIAILAIANLWSAWLAWLVTRRYSERFVQRGLAMVWFVAA
                     LGVVDSAWWLMFWGWASK"
     gene            complement(5726..6571)
                     /locus_tag="VHV99_24110"
     CDS             complement(5726..6571)
                     /locus_tag="VHV99_24110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009914340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HEX3638504.1"
                     /translation="MGQILFIVWRESVEALLVVGILYAWLKNGDDDARRGLPYLWGGV
                     VAGVIAAVALGAALVGFTEVLSGDAQDYFQTAMVLVACVLIVQMVLWMKQHGRTLKRD
                     MEQSLQKSTRDSNWWGVALLVALAIAREGSETVIFLYGLGFGQSGHVDGSQMLAVVIG
                     LGLAFLTYYILQLGGKFFSWRLFFRVTEIMLLFLGAGLFQTGVDKLIDKEILPTIIDQ
                     MWNSSAILDDSSTFGSLVATLTGYRAHPALMNLLAYAVYWAVVYLLVRRANRKPARQA
                     AGRAA"
     gene            complement(6654..6986)
                     /locus_tag="VHV99_24115"
     CDS             complement(6654..6986)
                     /locus_tag="VHV99_24115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006763873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX3638505.1"
                     /translation="MRINRTIAMFAASALLAGAAHAADLPTFKLEMNDGKLNPVRIEV
                     PAGQRIKIEIRNTGKGAAEFESVQLRKEKVLAPGGESFVVIAPQQPGEYKFFDDFHQQ
                     AQGVIVVK"
     gene            complement(7116..7664)
                     /locus_tag="VHV99_24120"
     CDS             complement(7116..7664)
                     /locus_tag="VHV99_24120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HEX3638506.1"
                     /translation="MRISSFVRGGAAAVAVAAAFSATAAEYPIGQHQIQGGMEIGAVY
                     LQPITMEPEGMMRKASDSDVHLEADIHAVKNNPTGFAEGDWMPYLQVRYELTKAGSSQ
                     TQKGDMMAMVANDGPHYGDNVKLQGPGKYHLKLIVEPPMQTGHMAFGRHVDKETGVGP
                     WFKPITLEYDFTYAGIGKKGGY"
     gene            complement(8006..10096)
                     /gene="uvrB"
                     /locus_tag="VHV99_24125"
     CDS             complement(8006..10096)
                     /gene="uvrB"
                     /locus_tag="VHV99_24125"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006053102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEX3638507.1"
                     /translation="MSEQHLTEADETLDESRFVTFEGSPFQLYQPYPPAGDQPTAIDT
                     LVEGVEDGLSFQTLLGVTGSGKTFTMANTIARLGRPAMVFAPNKTLAAQLYSEFREFF
                     PRNAVEYFVSYYDYYQPEAYVPQRDLFIEKDSSINEHIEQMRLSATKSLMERRDVIIV
                     GTVSAIYGIGNPSEYHKMILTLRTGDKLGQRDIIARLIAMQYNRNEADFQRGSFRVRG
                     DTIDIFPAEHAEMAVRIELFDDEVETLQLFDPLTGRVRQKIARFTVYPSSHYVTPRDT
                     VVRAVETIKTELRDRLEFFYSSGKLVEAQRLEQRTRFDLEMLQELGFCKGIENYSRHF
                     SGAAPGEPPPTLVDYLPSDALMLLDESHVLIGQLNGMYNGDRARKENLVDYGFRLPSA
                     LDNRPLKFNEFERKMRQVVFVSATPADYEQKTAGQVAEQLVRPTGLVDPEIEVRPART
                     QVDDVLAEINERVKVGDRVLVTVLTKRMAEQLTEFLADHGVKVRYLHSDIDTVERVEI
                     IRDLRLGTFDVLVGINLLREGLDIPEVSLVAILDADKEGFLRAERSLIQTIGRAARNV
                     NGKAILYADRVTDSMRRAIDETERRRAKQIAFNLENGITPRGVVKRIRDIIDGVYNVD
                     DARAELKEQQTRAKFEDMSEKQLAKELKRLEKQMMEHAKNLEFEKAAQTRDQLALLRQ
                     RVFGANVGDHVSGV"
     gene            10483..11682
                     /locus_tag="VHV99_24130"
     CDS             10483..11682
                     /locus_tag="VHV99_24130"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009932860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HEX3638508.1"
                     /translation="MSLFSAVELAPRDPILGLNEAFNAEARAPKVNLGVGVYFNEEGK
                     IPLLRAVRDAEKARVEAALPRGYLPIEGIAAYDAAVQKLLLGNDSPLIAAGRVVTAQA
                     LGGTGALKIGADFLKRLNPSAKVAISDPSWENHRALFESAGFEVVSYPYYDAHTQGVN
                     FDGMLSALNSYAAGTVVVLHACCHNPTGVDLSVDQWKQIVEVVKARNLVPFLDIAYQG
                     FGDNIDSDAAAVRLFAASDLNVFVTSSFSKSFSLYGERVGALSIITESKEESARVLSQ
                     LKRVIRTNYSNPPTHGGSVVAAVLASAELRATWETELGEMRDRIRAMRNGLVERLKAS
                     GVDRDFSFVNAQRGMFSYSGLTAPQVDRLREEFGIYAVSTGRICVAALNTRNLDAVAN
                     AIAHVLK"
BASE COUNT         2353 a         3707 c         3505 g         2185 t
ORIGIN      
        1 gccggcaagc aaacccgcaa gcgggcacgc ggcgccttac ccagcgcctg ccgaaaagcg
       61 caaaaaaccc ttgaaacaag ggccaaaaag taaaaggcct tcatcgtcag atgaaggcct
      121 tttttggtgc gcactcggcg ccttcgggcg gcgggatcat ttgccatcgg tcgaagaaga
      181 ttgatagctc gaatcaccgc atgcgacagc cgtgatcgac acggacagcg cggtcagccc
      241 tatcaggccg gctatcatag cggccatgag tttccgcata agagactcct tagcgaaggg
      301 atttcactat aggcccgcga gcgcgcgcat tcaagcaaac cgccattgaa agtactcatg
      361 acaagcggcg cttaaatgct aaaagcgatg ttgccgcacg aaataagtgc tatgacgcga
      421 acgcgagaac aggttctgcc attgtccgca cctcatgcgt cgggcactcg cccatgatgt
      481 gcttgccttc gtcgggatcg agcatttcga ccagatagtc gacgaacgcg cgcacagccg
      541 gcaccatgcc ctgacgcgac acgaatacgg cgtacaactg cggcgtcgga aaggtccaat
      601 ccggcattac cggcgacaac tggcccgcgc gcaacgcact gccgtacatc atctccggca
      661 acgcggcgat ccccacgccc gacaacaccg cttcacgaat cgtcatcaga tcggcggtga
      721 cgagacgcgg ttcgtgttcg tgcgcgtggc gcgtgccgtc gggggcaatc agatcgaaga
      781 cgtggcgtcc gtcggaggtc ggcacgtcga gcgtttcgaa acggctcaga tcggcaggca
      841 agagcggcgg cgcattctgc tgcaacaggc tcggcgcgcc aaccagcatc tgctgcgtgc
      901 gccacaacgg gcgcacgacg atgttggcgt tttccggcgg atcggagcgt acgcgcaacg
      961 cgatgtcgat cgaatcctcg aacagatcga ccacgcggtt cgtcacgcgc atgaccacgc
     1021 gtacttccgg aaagcgatgc atgaattccg gcaggatttg cgacaggatg gtctgcgaaa
     1081 tcgtcaccgg aacgctgaca cgcaccgtgc cgcgcggcga cgcacgcagt tgctgcacga
     1141 cgttgacggc cgcctgtgcc tcgctcagca ttgcctgaca gtgctggtag aagagctggc
     1201 cggcttcggt gagcgccagc ttgcgggtgg agcgctgtag cagacgtacg cccagcgagg
     1261 cttccaactc ggtcagccgg cgcgagaggc gcgacttgga aatgcccagc acgcgctcgg
     1321 cggcggaaaa accaccgtgt tcgacgacct gcgaaaaata catgaggtca ttcagattgt
     1381 gtgaatcgat cttcatctca tcgttccagt aatagaacaa tccattgcgt gacggcggct
     1441 ggcgcctagg aaaacggtcc ctataatatc gctatgtttc aaaaataacg ctattcccca
     1501 tttccggagg gacttttgat gagcacaacc cgcacgatcg aacgcacgtt tcccgctgtt
     1561 cgcacgaccg aaggtggcgg ctttatcgtt aaccgcccgt ttccgacccg cctgctgatg
     1621 gatttcgatc cgttcctgct gctcgacgaa atgggcccga tcgactacgc gccgggcgag
     1681 gccaaaggcg caccggatca tccgcatcgt ggtttcgaga cggtcacgta cgtgctcgag
     1741 gggcagttcg gccacaagga ttcggcgggc cattctggca ccctgcatgc cggcgacgtg
     1801 caatggatga cggcgggcgc gggcgtcgtg catagcgaga tgccggatcc ctcattcgtg
     1861 cgcacgggtg ggcgcgtgca cggcttgcag ttgtgggtga atctgccgcg gcgcgacaaa
     1921 atgatcgcgc cgcgttatca ggaaatgccg tcggccagca ttccggtcgc gacgtccgcg
     1981 gacggcaagg tgcgcgtcaa ggtgatcgcg ggcgaagcgc tcggcgtgaa ggccgcgatc
     2041 gaaacgcgca caccgatcct gtatcagcat ttctcgctgc agccgggcgc aacgatccag
     2101 caaccggtgc ccgccgacta tcgggtgttt gcctacagtc tgtcgggcaa gggcttttac
     2161 ggcgcagggg aaacgcgcca ggaaattggc gcccagacga tggtggtgtt tgagaacgac
     2221 ggagaatcgg tgacgctcgc agcgggcgag gagccgcttg aagtgctgct gctcggcggc
     2281 gtaccgctga aagaaccggt ggtgcgctac ggtcccttcg tgatgaacac ggaaggcgaa
     2341 atccgccagg ccgtgatcga ctaccaggca ggacgcatgg gcgcgatcac gcactgactc
     2401 gcttgagcac tgagcggcac cctgatgggg cgtgaccact cagacacgcc accatgccgc
     2461 ttcggcgcat gcaaagcggc gccgaagcgg gccaccgcgc cccgcccccg gcgcgaaggc
     2521 cgataaattc gttcacaata gcaattcatg ccgcgcgcca cgctgtctaa ccatgacaga
     2581 ccgtggcgcg cggcgatttc cgtccctgtt caggagcgcg catggcagag tccaccgtca
     2641 ccgcccatat cggttcgacg aatttccagg tccttttcga cgacggcaag cacacgtggc
     2701 tcgccgacga acccgagtcg cttggcggcg gcgatcgcgg gccgacgcct gtcgcgctgt
     2761 tgctgtcgag cctcggcgcc tgcacctcga tcacgctgaa aatgtacgct cagcgcaagg
     2821 gctggccgct cgccgacgtg cgcgtggcgc tgtcgctcga aaccggcgac gcgggctcga
     2881 ccatcgaccg caaaatcgta ctggaaggcg atctctccga cgagcagcgg gaacgactct
     2941 tgcaaatcgc caatgcgtgc ccagttcaca agatcctcac gcattcgatc acgatccgct
     3001 cgggtcttgc cgtcgcctga cgcgggaagg gccaggcggg ccggcaccgg ctaacggcgc
     3061 cgcacgtatt gcaacgcttt cataggaagc cgtcgttttg aatttcgaac accttatcca
     3121 gatcaacgat ccgttgaatc cgttggtcga ctcgatgacc cgcgagcagt tgtgggaggg
     3181 cctcgtgttg cgcgccgagc agccacagct gttcgtgata ggactcgaca gttgcaccat
     3241 tctttcgcgc gaaggcgaca cgctcgaacg ggaactgcac tacggccagg ccactgtgcg
     3301 cgaccgtgtc acgctgcagg atcgccagag cgtgcgctat gacattctgc cgaccgcgga
     3361 atacgtcggc ggctcgctga cgatgacgat cgagcagcct gacgacctgc aattgttcct
     3421 gcgcttcgaa tatgcgacga ccctgcccgt ctccgacgac caggacgctg tgcaaacgca
     3481 ggaaatcgtg aagtcggcat atcgcgagaa cgatatcgat accgtgcggc tgatccgtca
     3541 gtacgtgcag accaggcagg aacccggttc gttgcattga tgcgggtgcg ggcgctctgc
     3601 ccgcgcgccg tcgcgttgtt tggggcggcc tgatgggaga tggcctacct tcctgccccc
     3661 tttcattcag ctatcgaaag tcgaatccga tgaatttctc tttcttgtaa ataggaatag
     3721 ttatcattta gaattaatcc atcgaatcga ccaacagcta aaacgaatga ccgatctcac
     3781 gcacgcttcc acactcagcc tgcgccgccc ggcggccgcg ctgaccagcc gtccgaaggc
     3841 atcgacggca acggcaaccg ccgcgaaacc cgcagcggat cgcgccaacg gctcgggcga
     3901 cacgtcggat cgggtcgtgc gcagcgacac tttgctgcaa ggacatagcc acgtcagcat
     3961 cgtgcataac ggcgaaacct atcagctgcg tgcaacgcgc ttaggcaagc tcattttgac
     4021 gaagtaacag gcggcaacga atagcagtca gtaccaggta ttgtggggac cacctcctcg
     4081 agaggtgttg ggcattagcc agccacgacg gcttgcacgt gagaactaga ccccttcgtg
     4141 cagactccaa gccagccgtc gcagccagcc aggccgcttt tttggttctc acccaaggcg
     4201 gatgcacgaa acggcgtgtg tcgcggattc agaaattaaa aagccgtggg atggtttctc
     4261 ctccacggct ttttgttggc acggcgctgc cccgagtcgc gagcacgccg gagcattcag
     4321 acttacttcg aagcccagcc ccagaacatc agccaccagg cactgtcgac cacacccagc
     4381 gccgcgacaa accacaccat cgcaaggccg cgttgaacga agcgctcact atagcggcgc
     4441 gtaacgagcc aggcgagcca cgcgctccac aggttggcaa tcgccagaat cgcgatacgc
     4501 atgtccgacg cccaccacag cgacacatgt tccgcgcgca gcaacgacag cgtggtcgcc
     4561 gacaaaccga gaaacacccc cgcgccggca atcggaatca gaccttgcgt caggtgatgc
     4621 aaacgcacgc tgctgaaacg ccccagcatg cgcgtcgcgc ccatcagcaa caccagcagt
     4681 accgtgccat acaccagcgc cgtggcgagg atatagccga tcaccatcgt gccgtcgagc
     4741 cacgtgaaca catcgttctg ctcggggtag tgcgtgaaca gaaaccacgg cgcgttggtg
     4801 tcgagcggcc acgtgatgtc gtgatcgacc agccatgttg cgaagaacat cttcaggtcg
     4861 acaaaccagc gcgaagcggt ccagtgaaac gcgccgatcg cgatgccgag caagccgtac
     4921 aggatcaacg cggtatccca cgggttggcc tgcctgtcgc cgagctgcac gacttcagac
     4981 gacggcgcac gccacgtcag cgcgatcgcg tcgcgatgtc cgctgcagcg gccgcacata
     5041 tgacagtccg aggcgccctt catattgcgc agcggcacca gcggcgcgca attgatcgga
     5101 atcacgcgat gcccatgttc gccgttcttg tacgaacgac gccacgcgtc ttcatcgacc
     5161 ttgtagtgaa acggcgcgag acgcgccaga agcgaaaaaa ctccgttgac ggggcacaga
     5221 tacttgcacc agacacgctt ctcgcgtccg tacagcaagc cgatgatcat cgccgcaaag
     5281 gtcgagccgc cgagcaccag caacaccgcc ttcgggtact ggtagacgct gaccatctga
     5341 ccgtagatgg tggtgatacc gaacgcgacg aacggccagc cgccccagcg catccagcgc
     5401 ggaatcgccc agccgcggcc gtatttgctg gcgaattcgg cgagtgcacc ctccggacac
     5461 agtacgccac accagacgcg gccgagcatc accatcgaca gcagcacgaa cggccaccag
     5521 atgccccaga acacgaactg cgcggcaagc gtcaggttat tccacagatg cgcggtgtcg
     5581 tccggcaggg gcatcaccgc cggcacgagg atcaggaacg cgtataccgc cacgaccacc
     5641 cattgaattc cgcggatcgc agcgccgtgg cgctgcatcc actgaccggc cgcagcgagg
     5701 cgccccgggc gggtcatcac ggtgctcatg ctgcgcgccc tgcggcctgc cgcgccggct
     5761 tgcgattcgc gcgacgcacc agcagataga cgaccgccca gtacacggcg taggcaagca
     5821 gattcatcag cgccgggtgg gcgcgataac cggtgagcgt cgccaccagt gaaccgaacg
     5881 tgctcgagtc atcgaggatc gccgacgaat tccacatctg gtcgatgatt gtcggcagga
     5941 tttccttgtc gatcagcttg tccacgccgg tctggaacag acctgcgccg agaaacagca
     6001 acatgatttc agtgacgcga aagaagagcc gccacgagaa gaacttgccg cccaactgca
     6061 agatgtagta agtcaggaac gcgagaccga ggccgatcac gaccgccagc atctggctgc
     6121 catccacatg acccgattgg ccgaaaccga gaccgtacag gaagatcacc gtttcgctgc
     6181 cctcacgcgc gatcgccagt gcgaccagca acgcgacgcc ccaccagttc gaatcgcgcg
     6241 tgctcttttg cagcgactgt tccatgtcgc gcttgagcgt gcggccgtgc tgcttcatcc
     6301 acagcaccat ctgcacgatc agcacacacg cgaccagcac catcgcggtc tggaaataat
     6361 cctgtgcgtc accggagagc acttcggtga aaccgaccag cgccgcgcca agcgcgaccg
     6421 ccgcgatcac accggccacc acgccgcccc atagatacgg caaaccgcgg cgcgcatcgt
     6481 cgtcgccatt tttcagccac gcgtacagga tgccgacgac cagcaaggcc tcgacacttt
     6541 cccgccacac gatgaataga atctgaccca tccaagctct cccttctact cttctattgc
     6601 tttggcgaac gtgtgacccg actgctcaag gcccgcgctc gcatcccaag ccgttacttc
     6661 acgacgatca cgccctgcgc ctgctgatgg aaatcgtcga agaacttgta ctcgcccggc
     6721 tgctgcggag cgatcacaac gaacgactcc ccgcccggcg ccaacacctt ttctttacgc
     6781 aattgcacgc tttcgaattc ggcggcgcct ttgccggtgt tgcggatttc gattttgata
     6841 cgctgccccg ccggcacttc gatacgaacc ggattcagct tgccgtcgtt catttccagc
     6901 ttgaaggtcg gcagatccgc tgcgtgcgcg gcgcccgcca gtaaagcgga cgcggcgaac
     6961 attgcgatcg ttcggttaat tctcattagc cccttccgga aaacgcggcg cgatgggttt
     7021 catgacccgc acgccgcctg ctgcattcgt tcacccaccg acgcgcggcc acgcagagat
     7081 gcgtggcgaa ccgtgatgat ctgtgtccgc ggcgctcagt agccgccctt cttgccgata
     7141 cccgcgtacg tgaagtcata ttcgagcgta atgggcttga accacggacc cacgccggtt
     7201 tccttgtcga cgtggcggcc gaaagccata tggccggttt gcatcggcgg ttcgacgatc
     7261 agcttcaggt ggtacttgcc cgggccttgc agcttgacgt tgtcgccgta atgcggaccg
     7321 tcgttggcga ccatcgccat catgtcgccc ttctgggtct ggctcgaacc cgctttcgtc
     7381 agttcataac gcacttgcag atacggcatc cagtcaccct cggcgaaacc cgtcgggttg
     7441 ttcttgactg cgtggatatc tgcctcgagg tgaacgtcgg aatccgacgc cttgcgcatc
     7501 atgccttccg gttccatggt gatgggctgc agatagaccg cgccgatttc catgccgccc
     7561 tggatttgat gttggccgat cgggtattcc gctgccgttg ccgagaaggc cgctgctacg
     7621 gccacggctg cggcgccgcc acgcacaaat gaagaaatcc gcattgaaac tccttgtttt
     7681 tttatctgac cggaatcgaa cccattggaa cagtgcgttg cgcacgtctt ggtgcaacgg
     7741 cccaagcctt aaagagggcg attgaccgta agcaaaccgc atatgcgaac gttaatgcga
     7801 accattctca atattggtca agtttatcat tgttcgtcag ggaggacaaa tttggcggga
     7861 ggctaagcct tatggcagca gggtcttaag gccgtcttaa ggaatcgggg ccagagaggt
     7921 gatggcggga cgtctggcgt gttgccaggc tcgatgaggc tgggcacggc ccattgccgc
     7981 gcccagagcg gaagcgcgct tttttttaga cgccggacac gtgatcgcca acgttcgcgc
     8041 caaacacgcg ctgacgcagc agcgctagct ggtcgcgagt ctgtgcggcc ttttcgaact
     8101 cgagattctt cgcgtgctcc atcatctgct tttcgagacg cttgagttcc ttggcgagct
     8161 gcttctcgga catgtcctcg aatttcgcgc gcgtttgctg ttccttcagt tcggcgcggg
     8221 cgtcgtcgac gttgtacacc ccgtcgatga tgtcgcgaat gcgcttcacc accccgcgcg
     8281 gtgtaatccc gttttcgagg ttgaaagcga tctgcttggc gcgccgccgc tcggtttcgt
     8341 cgatcgcgcg acgcatcgag tcggtcaccc tgtccgcata cagaatcgcc ttgccgttca
     8401 cgttacgcgc ggcccggccg atggtctgga tcagcgagcg ctcggcgcgc aggaagcctt
     8461 ccttgtccgc gtcgagaatc gccaccagcg agacttccgg aatatccagc ccttcgcgca
     8521 gcaggttgat cccgaccagc acgtcgaatg tgccgagccg caaatcgcga atgatttcga
     8581 cccgttccac cgtatcgatg tcgctgtgca ggtaacgcac cttgacgccg tggtcggcca
     8641 gaaactcggt cagctgctcg gccatccgct tggtcagcac cgtgaccagc acgcggtcgc
     8701 cgaccttgac gcgttcgttg atttccgcga gcacatcgtc gacctgcgtg cgcgccggcc
     8761 tcacctcgat ttccggatcg acaaggccgg tcggacggac cagctgctcg gccacctgcc
     8821 ctgccgtttt ctgctcgtaa tcggccggcg tagccgacac gaacacgacc tgacgcatct
     8881 tgcgctcgaa ctcgttgaac ttgagcgggc gattatcgag tgccgacggc aggcgaaaac
     8941 cgtagtccac caggttctct ttgcgcgccc ggtcaccgtt gtacatgccg ttcaactggc
     9001 cgatcagcac gtgcgattcg tcgagcagca tcagcgcatc ggagggcagg tagtccacca
     9061 gcgtgggcgg cggctcgcct ggcgccgcgc ccgagaagtg ccgtgagtag ttctcgatgc
     9121 ccttgcagaa gcccagctcc tgcaacattt ccagatcgaa ccgcgtacgc tgctcgagcc
     9181 gctgcgcttc gacgagtttg ccgctgctat aaaagaattc gagccggtcg cgcagttcgg
     9241 tcttgatcgt ttcgaccgcg cgcaccacgg tatcgcgcgg cgtcacatag tgcgacgacg
     9301 ggtacacggt gaagcgggca atcttttgcc gcacgcggcc ggtgagcgga tcgaacagtt
     9361 gcagcgtctc gacctcgtcg tcgaacaact cgatgcgcac cgccatttcg gcgtgctcag
     9421 ccgggaaaat atcgatcgtg tcgccgcgca cgcggaacga accgcgctgg aagtcggctt
     9481 cattgcggtt gtactgcatc gcgatcagcc gggcgatgat gtcgcgctgg ccgagcttgt
     9541 cgccggtacg cagcgtcaga atcatcttgt gatattccga cgggttaccg ataccgtaaa
     9601 tcgccgacac cgtaccgacg atgatcacgt cgcgccgctc catcaggctc ttggtggccg
     9661 acaggcgcat ctgctcgatg tgctcgttga tcgacgaatc tttctcgatg aacagatcgc
     9721 gctgcggcac gtacgcttcc ggctggtagt agtcgtagta cgagacgaag tactcgacgg
     9781 cattgcgcgg aaagaactcg cggaactcgg aatagagctg cgcggcgagc gtcttgttcg
     9841 gggcgaacac catcgccggc cggccgagcc gcgcgatggt attggccatc gtgaaggttt
     9901 tgccggagcc ggtcacgccg agcagcgtct ggaacgacaa accgtcttcc acgccttcga
     9961 cgagcgtgtc gatcgccgtc ggctggtcgc cggcgggcgg atacggctga tacagctgga
    10021 acggcgagcc ttcgaacgtg acgaatctgg attcgtcgag cgtctcgtcg gcttcagtca
    10081 gatgttgttc ggacatgggg agcggcaccg ggccttgggc aaagaaccat tctaacgggt
    10141 tgcgggaacc cgggcggagc gggtctgcgt agggggttgg ggcggccgcg cgatgcttgt
    10201 ggcccttttt gcggggattg cttggggttt gcgtggggct tgctcgcgac cgtcccgaac
    10261 agatcgatta ggagcacatg acgcctgagt gcctgttttt caagcaaata attcagtcca
    10321 acggacgttg tcgtccgaaa aatgcccgcg gattcgctac aatgacaagt tgcgctcgct
    10381 cgccgatgcc cgccgccgcg gtctgaattg tccgtcagac acctttggcc gggccgcccg
    10441 cgatcgaaac cgccctcttc tcactactgc tgctcaccca tcatgtctct gttctccgcc
    10501 gtcgaacttg ctccccgcga cccgattctg ggcctgaacg aagccttcaa cgccgaggcg
    10561 cgcgcgccca aggtcaacct tggcgttggt gtgtacttca atgaagaagg caagattccg
    10621 ctgctgcgcg ccgtgcgcga tgcggaaaag gcacgcgtcg aagccgcgct gccgcgtggc
    10681 tatctgccga tcgaaggcat cgccgcctac gacgccgccg tacaaaagct gctgctcggc
    10741 aacgactcgc cgctgatcgc cgcaggccgc gtcgtgacgg cgcaagcact gggcggcacg
    10801 ggcgcgctga aaatcggcgc cgacttcctg aagcgcctga acccttcggc caaggtcgcg
    10861 atcagcgatc cgagctggga aaaccaccgc gcgctgttcg aaagcgccgg cttcgaagtc
    10921 gtgtcctacc cgtactatga cgcgcacacg cagggcgtga atttcgatgg catgctgagc
    10981 gcgctgaaca gctatgcggc gggcacggtt gtcgtgctgc acgcgtgctg ccataacccg
    11041 accggcgtcg acctgagcgt cgaccaatgg aagcagatcg tcgaagtcgt caaggcgcgc
    11101 aatctggtgc cgttcctcga catcgcgtat caaggtttcg gtgacaacat cgattccgac
    11161 gctgcagcag tccgtctgtt cgcggcatcg gacctgaacg tgttcgtgac atcgtcgttc
    11221 tcgaagtcgt tctccctgta cggcgagcgc gttggcgccc tgtcgatcat cacggaaagc
    11281 aaggaagaat cggctcgcgt gctgtcgcaa ctgaagcgcg tgatccgcac caactattcg
    11341 aacccgccta cgcacggcgg ctcggtggtc gcagcggtgc tcgcgtcggc ggaactgcgc
    11401 gccacgtggg aaacggaact cggcgaaatg cgcgaccgca ttcgcgccat gcgtaacggc
    11461 ctggtcgaac gtcttaaggc aagcggcgtg gatcgcgatt tcagcttcgt gaacgcgcaa
    11521 cgcggcatgt tctcgtactc gggtctgacc gcgccgcaag tggaccgtct gcgtgaagag
    11581 ttcggcatct acgctgtgag cacgggccgc atctgcgtgg ctgcgctaaa cacgcgcaac
    11641 ctcgatgctg tcgccaacgc gattgctcac gttctgaagt aatggtttga gcaagagcgg
    11701 caaagttaag cgccgctcga gttctgcaag gttgcatgcg tgaatgaaaa
//
LOCUS       DATASH010000904         3036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_7076140,
            whole genome shotgun sequence.
ACCESSION   DATASH010000904 DATASH010000000
VERSION     DATASH010000904.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7076140"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..163
                     /locus_tag="VHV99_24135"
     CDS             <1..163
                     /locus_tag="VHV99_24135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006400312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HEX3638509.1"
                     /translation="DGDGWDHGQILDPENGKLYKCKMHLEDGGNKLVVRGYIGVSLLG
                     RSQTWVRQQ"
     gene            complement(331..789)
                     /locus_tag="VHV99_24140"
     CDS             complement(331..789)
                     /locus_tag="VHV99_24140"
                     /inference="COORDINATES: protein motif:HMM:NF024736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX3638510.1"
                     /translation="MNPYRDINDEEWQRIAPLLPELRPRSELRGRPLANTRSVLNGVL
                     WVMYSGATWSAMPRKYPSYQTCHRRFKAWYESGVLKRVMDQLFGAASEELCQMMESRM
                     RTHVSAQQKSADAEKVPVMAPTASTAYSPGSTKPLPSSPFAFSSPFKHAA"
     gene            complement(952..2049)
                     /gene="zapE"
                     /locus_tag="VHV99_24145"
     CDS             complement(952..2049)
                     /gene="zapE"
                     /locus_tag="VHV99_24145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011402415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HEX3638511.1"
                     /translation="MNVTEYYEKELQTRGYQSDPAQRAAVDRLQRCYEEWVAYKARRS
                     NAFKKLINRPDPPRGVYMWGGVGRGKSFLMDSFYMIVPVQRKTRLHFHEFMREVHREL
                     EELKGTADPLDELARRIAKRYRLICFDEFHVSDIADAMILYRLLDKLFENGVQFVMTS
                     NYDPDTLYPDGLHRDRMLPAIELIKTKLDVINVDAGVDYRQRTLAQVEVYHTPLGAAS
                     DKALRDAFSRLAAVPDESPILHIEKRELKALRRADGVVWFDFATLCGGPRSQNDYLEL
                     ASRFHAVILSGVPQMSVRMASEARRFTWLIDVFYDHKVKLLMSAAVPPEQLYVDGPMA
                     NEFTRTVSRIVEMQSKEYLDTPRRIVDTSLT"
     gene            complement(2140..>3036)
                     /locus_tag="VHV99_24150"
     CDS             complement(2140..>3036)
                     /locus_tag="VHV99_24150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3638512.1"
                     /translation="TFDSVPKKLAVIGAGVIGLELGSVWRRLGSDVTVLEALPEFLGA
                     ADQALAKEAAKQFKKQGLDIHVGVKVGEIKTTDKSVTINYTDKDGNAQTLEADRLIVS
                     IGRVPNTDNLGLEAIGLKANERGFIDVDDHCATAVPNVYAIGDVVRGPMLAHKAEDEG
                     VLVAEIIDGQKPHIDYNCIPWVIYTEPEIAWVGKTEQQLKAEGREIKTGQFPFMANGR
                     ALGINKADGFVKMIADAKTDELLGVHIISANASDLIAEAVVAMEFKAASEDIGRICHP
                     HPSLSEVMREAALAVDKRALNM"
BASE COUNT          578 a          916 c          902 g          640 t
ORIGIN      
        1 ggacggcgac ggctgggatc acgggcagat tctcgacccg gaaaacggca agctctacaa
       61 gtgcaagatg cacctggaag atggtggaaa caagctggtg gtgcgtgggt atattggcgt
      121 ttcgctgctg ggccgctcgc agacctgggt tcgtcagcaa tagtcagcga tagtcagcaa
      181 actgagcgaa tcattcaggc gtcgacgcct gacgcgcgat ggtggtgttc catcaaggcc
      241 gctactgctg ccacgatgga accataaaaa accggccgac gattgctatc gtccggccgt
      301 tttttgtttg agtctggctg gttggcgttg tcatgcagcg tgcttgaagg gcgacgaaaa
      361 agcgaacggc gaagacggta acggcttggt cgatcccggg ctatacgccg tggaagcggt
      421 gggcgccata accggaacct tttctgcgtc agcgctcttc tgttgcgcgc tcacatgcgt
      481 gcgcatgcgc gattccatca tctgacagag ttcctcgctc gcggcaccga acagttgatc
      541 catcacgcgc ttgagcacgc cggattcgta ccatgccttg aagcggcgat ggcaggtttg
      601 atacgagggg tatttacgcg gcatggcgga ccaggtcgcg ccgctataca tcacccacag
      661 cacgccgttg agcactgagc gcgtattggc gagcggccgg ccacgcagtt cggaacgcgg
      721 acgcagttca gggagcaacg gtgcaatgcg ttgccattct tcatcattga tatcacggta
      781 cggattcatg gcttctcctt tgttagaacc atgacaaaga agatatccag tcgtccgcgc
      841 acggaatata agatcaatcc gaatcttgaa aatacggctt cgaacactgc cgattaggcg
      901 agcggaccgt gacctatcgc aaaagcatcg ccgcccgctt gacgcggccc ctcaggtcag
      961 cgacgtatcg acgatccggc gcggcgtgtc gagatattct ttcgattgca tttcaacgat
     1021 gcgcgacacc gtacgcgtga attcgttggc catcggaccg tccacataga gttgctcggg
     1081 cggcacggca gccgacatca gcagcttgac cttgtggtcg taaaacacgt cgatcagcca
     1141 ggtgaaacgg cgcgcttccg aagccatccg caccgacatc tgcggcacgc cggacaggat
     1201 caccgcgtga aagcggctcg ccagttccag gtagtcgttc tgcgagcgcg gaccgccgca
     1261 caacgtcgcg aaatcgaacc acacgacgcc gtcagcacgc cgcagggcct tcagctcccg
     1321 cttttcgatg tgcaggatag gactctcgtc cggaaccgcc gcaaggcgtg aaaacgcgtc
     1381 acgcagggct ttgtccgaag ccgcgcctag cggcgtgtga tacacctcga cttgcgccag
     1441 tgtgcgctgc cggtagtcga cacccgcgtc gacgttgatc acgtcgagct tcgtcttgat
     1501 gagttcgatc gccggcagca tgcgatcgcg atgcaggccg tccggataca gcgtgtccgg
     1561 atcgtagttg gacgtcatca cgaactgcac gccgttctcg aatagtttgt cgagcaggcg
     1621 atacaggatc atcgcgtcgg caatatccga tacgtggaat tcgtcgaaac agatcagccg
     1681 gtagcgtttg gcaatgcgcc gcgcgagttc gtcgagcgga tcggccgtgc ctttcaactc
     1741 ttccagttcg cgatgcacct cgcgcatgaa ctcgtgaaaa tgcagacgcg ttttacgctg
     1801 caccggcacg atcatgtaga agctgtccat caggaagctc ttgccccgcc ctacgccgcc
     1861 ccacatgtac acgccgcgcg gcggatccgg gcggttgatc agtttcttga acgcattcga
     1921 gcggcgcgcc ttgtatgcga cccactcttc atagcaccgc tgcagacggt cgactgccgc
     1981 gcgttgcgcc gggtccgatt gataaccgcg cgtctgcagt tctttctcgt agtattcggt
     2041 gacgttcatt gtgttgctgc aaaaagaaag gcgggaggga gtgaacccgc ccgccttggt
     2101 ggtgatgcca gaattgcgtt cagtcaggcg tgcgtgtgat tacatgttca gcgcgcgctt
     2161 gtccacggcc agtgccgctt cgcgcatgac ttccgacaac gacgggtgcg gatggcaaat
     2221 gcggccgata tcttccgacg ccgccttgaa ttccatcgcc accacggctt ccgcgatcag
     2281 gtcagaggcg ttcgcagaga tgatgtgcac gccgagcagt tcgtcggtct tcgcgtcggc
     2341 gatcatcttg acgaaaccat ccgccttgtt gatgcccagc gcgcggccgt tcgccatgaa
     2401 cgggaactga cccgtcttga tctcgcggcc ttcggccttc agttgctgct ccgtcttgcc
     2461 gacccacgcg atttccggtt cggtgtagat cacccacgga atgcagttgt agtcgatatg
     2521 cggcttctga ccgtcgatga tttcagcgac cagcacgcct tcgtcttccg ccttgtgcgc
     2581 gagcatcggg ccacgcacca cgtcgccgat cgcgtacacg ttcggcaccg ccgtcgcgca
     2641 gtggtcgtcg acgtcgataa agccgcgctc gttcgccttc aggccgatcg cttccagacc
     2701 gaggttgtcg gtgttcggca cgcggccgat cgacacgatc aggcggtcgg cttcgagcgt
     2761 ttgcgcgttg ccgtccttgt ccgtgtagtt gatcgtgacg ctcttgtccg tggtcttgat
     2821 ttcgccgacc ttcacgccga cgtggatgtc caggccttgc ttcttgaatt gcttggcagc
     2881 ttccttggcc aacgcctgat ctgccgcgcc gaggaattcc ggcagcgctt ctagcacggt
     2941 cacgtccgaa ccaagacggc gccacaccga gccgagttcc aggccgatca caccggcgcc
     3001 gatcacggcg agtttcttcg gcacggagtc gaacgt
//
LOCUS       DATASH010000905         8182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1143155,
            whole genome shotgun sequence.
ACCESSION   DATASH010000905 DATASH010000000
VERSION     DATASH010000905.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 8182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8182
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1143155"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            538..1533
                     /locus_tag="VHV99_24155"
     CDS             538..1533
                     /locus_tag="VHV99_24155"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX3638513.1"
                     /translation="MTPFSVLDLSPVNTGATPADAFKNTLDLAQHAENWNYRRFWLAE
                     HHNMTGIASAATSVVIGYVAGGTKKIRVGSGGVMLPNHAPLVIAEQFGTLASLYPDRI
                     DLGLGRAPGTDQSTARALRRDLQNSADSFPDDVVELQRYFADPVPGQRIRAVPGAGLD
                     VPLWLLGSSLFSAQLAAALGLPFAFASHFAPDYMFSALKVYREQFRPSATLDKPHAMV
                     GVNLFAADSNEEAQHLFTSLQQQFINLRRGTPGQLQPPVERLEASEMELKGVAHSLAC
                     SVIGDRDTVREGLLSIIDQTGADELMLTAQIYDHTARLRSFEIAAQVREELSAGK"
     gene            complement(1609..2793)
                     /locus_tag="VHV99_24160"
     CDS             complement(1609..2793)
                     /locus_tag="VHV99_24160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3638514.1"
                     /translation="MNFRRQSLFAMLSACVLAAAPGVFAATPASAAVADGPASAVGAV
                     SAWRTASGPASASSTVAQPDFAAAGVAPAAAADNSGPAYGPELQGFNYPAPVEQYDFA
                     SQGVALHMAYMDIKPEHANGRTAVLLHGKNFCAATWDATIHRLSDAGYRVIAPDQIGF
                     CKSSKPEHYQYSFQQLARNTHALLASLGVTDATMIGHSTGGMLAIRYALMYPHETQQL
                     VLVNPIGLEDWKAKGVPSLSVDQWYERELKTTADGIRRYEQATYYAGQWRADYEPWVQ
                     MLAGMYRGPGKQIVAWNSALLDDMIYTQPVVYELSHLTMPTLLLIGQKDTTAIGKDAA
                     PPEVRAKIGHYPELGKAAAKAIPHATLVEFAGSGHAPQMQDPQAFYKALLEGLAVVPA
                     NR"
     gene            3357..4622
                     /gene="glgC"
                     /locus_tag="VHV99_24165"
     CDS             3357..4622
                     /gene="glgC"
                     /locus_tag="VHV99_24165"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEX3638515.1"
                     /translation="METPARLNDLQRTTLAIVLAGGRGTRLGPLTNKRVKPAVHFGGK
                     YRIIDFALSNCLNSGIRRIAVVTQYKAHSLLRHMQRGWGFLRGEFGEFIDLWPAQQRV
                     EGAHWYRGTADAVFQNLDIIRSIRPKYVVVLAGDHIYKMDYTRMIADHAESGADCTVG
                     CIEVPRMEAVAFGVMAVDENRRVTGFVEKPADPPAMPGRPDSALASMGIYVFSSDYLY
                     SLLEENISSVDTDHDFGKDILPRVVTQGTAIAHPFSMSCVSSSPDVEPYWRDVGTIDA
                     YWAANLDLASTIPTLDLYDRNWPIWTYQEQLPPAKFVRDMKGLQGSGNNLIVCGGCVI
                     SGSQISRSVLSSNVKVSSFCNINEAVLLPQVTVGASCRLQKVVVDRGCAIPDGTVIGE
                     DPVADAERFYRTDDGVVLVTPEALRQKGT"
     gene            4750..6210
                     /gene="glgA"
                     /locus_tag="VHV99_24170"
     CDS             4750..6210
                     /gene="glgA"
                     /locus_tag="VHV99_24170"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEX3638516.1"
                     /translation="MTIRALHVASELYPLLKTGGLADVAGALPPALSELGADVRVLLP
                     GFPAVVAGLTDLRPVAQLGRRFDSPGVTLEQGTLPSNGLIVYVIRAGTLYDRPGNPYL
                     NDEHVPYGDNAQRFALLGWVAAQLAQHLDPAWAPQIVHAHDWHAGLAPAYLKAAERQH
                     GRSFARTVFTVHNLAYQGVFPAHLFGQLALPDDFFSMHGVEFYGQLSFLKAGLYYSDR
                     ITTVSPTYAREIQTLAQGGGLDALLRHRTHDLSGILNGVDYAVWNPATDALLEDHYTA
                     SRLAGKLACKEALQKRFGLAQKSDALLFGVVSRLTEQKGLDLLLETVPEIVKRGGQLV
                     VFGTGDPALEHGLKRVAHAHPESVAVELGFDETLAHTIVAGSDVIAVPSRFEPCGLTQ
                     LYALAYGSLPLVHCVGGLADTVVDASLENLADDLATGFVFERFEPKGLTAAIRRAFAL
                     HQRRTEWKATQRRAMRQDFGWGASAERYLTLYRELA"
     gene            6523..7389
                     /gene="pdxY"
                     /locus_tag="VHV99_24175"
     CDS             6523..7389
                     /gene="pdxY"
                     /locus_tag="VHV99_24175"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HEX3638517.1"
                     /translation="MTKNVLSIQSHVVFGHAGNSAAVFPMRRLGINVWPLNTVQFSNH
                     TQYGHWTGGAIEASQMEELVEGIGAIGMLPRCDAVLSGYLGTPEQAQSVLEIVKAVKA
                     ANPRAWYFCDPVMGAASGCKVEPGIQEFLVRTMPEMADAMAPNHTELQRLVGREIDTL
                     EEAVTACREVIARGPKLMLVKHLLDRNSPADRFNMLVVTEREAWMGQRPLYPFARQPV
                     GVGDLTSAVFVARTLLGDSIRAAFEHTLAAVNAVVKATWQAGRYELELVAVQNEIAQP
                     REWFDAWVAETA"
     gene            7550..8011
                     /locus_tag="VHV99_24180"
     CDS             7550..8011
                     /locus_tag="VHV99_24180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018418547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638518.1"
                     /translation="MDGYIRSEREEFFEQLCMSVDADEAHEQEAIEYFENQFDQADFD
                     PAQWLDIALYYSPAVARGIVDMVTPDDKARSNIAEVIADNLDISYGEDECQQFAETIE
                     FALNNGVPVDLDLVLDGCQRAIDDLDTWADEETKAPLLRLREELLRQQGER"
BASE COUNT         1574 a         2681 c         2502 g         1425 t
ORIGIN      
        1 gggcgcatcg ccgagacaag gccgatggct cccacctgct cccaaacgaa gccgctggtt
       61 tgcatgatga acccttctgg cgagcacgca gggcgaggcg ggaagtatga cagccttgtc
      121 actcgcgccg aatgtgcgag agcacggatt cgagatgcac cactaccgcg actgcgcggg
      181 ggacccgctt atgggctggc ggtgtgagcc gctttctttt gcctactttt ctttgcggca
      241 ggcaaagaaa agtaggtgcc cgccccgcac aggggcgacg ctaatagacc actatcaatt
      301 caaggaaagg ccaacaccat aggcagacag acaaacaagc accacgcatg caaccaactc
      361 aacaacgggc caacgccgca ggcaaccaga caaccaaccg ccccgcagcc accctgaaca
      421 ggcaaaacca acacccctcc cacgaacgtg gacatccccc accatgagca gaaaacccaa
      481 acctttgcca caatgcccca tcgcgcgaaa gcgcccgaaa ccgctaatga acagaaaatg
      541 acccccttct cggtcctaga tctatcgccg gtcaacaccg gtgccacccc cgcggacgcg
      601 ttcaaaaaca cattagacct cgcccagcac gcggaaaact ggaactaccg ccgcttctgg
      661 ctagcagaac accacaacat gaccggcatc gcgagcgcgg ccacctcagt ggtgatcggc
      721 tacgtcgccg gtggaacaaa gaaaatccgc gtcggctccg gcggcgtcat gctgccaaac
      781 cacgcaccgc tagtcatcgc ggaacaattc ggcaccctcg cctcgctcta ccccgatcgt
      841 atcgacctgg gcctcggccg cgcccccggc accgatcaaa gcaccgcccg cgcactgcgc
      901 cgcgatctgc aaaacagcgc cgactctttc cccgacgacg tcgtcgagct gcaacgctat
      961 ttcgccgacc ccgtgccggg ccagcgcatt cgcgcagtcc ccggcgcagg tctcgatgtg
     1021 ccgctgtggc tgctcggatc gtcgctgttc agcgcacaac tggctgcggc tctcggtctg
     1081 ccgttcgcgt tcgcatcgca cttcgcgccc gattacatgt tcagcgcgct caaggtctat
     1141 cgcgaacaat tccgcccgtc ggcgacgctc gataaaccgc acgccatggt cggcgtcaac
     1201 ctgttcgccg ccgatagcaa cgaagaagca cagcatctgt tcacctcgct gcagcagcag
     1261 ttcatcaacc tgcggcgtgg cacgcccggt cagttgcagc cgccggtcga acgtctcgaa
     1321 gcgtcggaga tggagttgaa gggtgtcgcg cactcgcttg cctgctccgt gatcggcgat
     1381 cgcgatacgg tacgcgaagg actgctgtcg atcatcgatc agaccggcgc ggatgaattg
     1441 atgctgaccg cgcaaatcta cgatcacaca gcgcggctgc gctcgttcga gatcgccgcg
     1501 caagtccgcg aagagttgag cgccggtaag taacgtcgcc ggcaagcaag aaaaaagggc
     1561 ggcctgtctt aacaaaggcc gcccttcttt ttttgtcgac gccaaaactc accgatttgc
     1621 cggcaccacc gccagcccct cgagcagcgc cttataaaac gcctgcgggt cctgcatctg
     1681 cggcgcgtgc cctgaaccgg cgaattcgac gagcgtcgcg tgcggaatcg ccttcgcggc
     1741 cgccttgccc aactccgggt aatggccgat cttcgcgcgc acctcgggcg gcgcggcgtc
     1801 cttgccgatc gcagttgtgt ccttctggcc aatcagcaag agcgtgggca tggtcaggtg
     1861 actcagctca tacaccacag gctgcgtgta aatcatgtca tccagcagcg cggaattcca
     1921 cgccacgatc tgtttgcccg ggccgcggta catgcccgcc agcatctgca cccacggctc
     1981 atagtccgcc cgccattggc ccgcgtaata ggtggcctgc tcgtagcggc gaatgccgtc
     2041 cgcggtcgtc ttcagttcac gctcatacca ttggtcaacc gataacgacg gcacaccctt
     2101 cgccttccag tcctcgaggc cgatcggatt gaccagcacc agttgctggg tttcatgcgg
     2161 atacatcaac gcatagcgga ttgcgagcat gccgccggtc gaatggccga tcatggtcgc
     2221 gtcggtcacg ccgagcgatg cgagcagcgc atgcgtgttg cgcgccagtt gctgaaaact
     2281 gtactgatag tgctcgggct tgctcgactt gcaaaagccg atctggtccg gcgcgatcac
     2341 ccgatagccg gcgtcgctca aacgatgaat ggtcgcgtcc cacgtcgccg cgcagaaatt
     2401 cttgccatgc agaagcacgg ccgtgcggcc gttcgcatgc tccggcttga tatccatgta
     2461 cgccatgtgc agcgccacgc cttgcgaggc gaagtcgtac tgctcgactg gcgccggata
     2521 gttgaagccc tgcagctcag ggccgtacgc cgggccgctg ttgtccgcgg ccgctgcggg
     2581 agcgacaccc gcagcagcga aatcgggctg ggcaacagta ctggaagcag aagcaggacc
     2641 ggaagcagta cgccaggcac taaccgcacc aacggcacta gcaggaccat cggccaccgc
     2701 ggcactggcc ggcgtggcgg caaagacacc tggcgccgcg gctagtacgc aggcgctcaa
     2761 catggcgaac aaagactggc ggcgaaaatt catcgattgt tttcttgtaa tgagaaatca
     2821 gcggacaaca tgcaggcctc gcgcgccgac gagccaacgc gcgcaccccg ttcagagcgc
     2881 acgattctac gacgcgccgt ccgactctgc tgccgcacgc gggcgctgac ggcggagccg
     2941 gccggtttgg cacgcatatt gcggcgctgc acctcgcacc ctatgtggcg tggcgcacgg
     3001 atgcctgggg tgcaagggtt gatcgtgcgc aagtaggcag gcgaccggca tccgcacaat
     3061 tcacactact cccccgcggc aaatcagtgt tagaactagc agacggggcc gcgaagacgg
     3121 cgccgcccgg cagcacgaat catcagcgga gagcagcgga acagaagcag gcgaacaaga
     3181 catccggggc atgctaatca gcaactatct tgcatgggac cggagtaagc gccatagggc
     3241 tggagcagac gctactaaag cgtcaacttc agccgacacg ctaactccag tcgatgcgct
     3301 gcatggcacc ggggtaagcg ctaaagcgcc aactccggtc gacaaaggag acagacatgg
     3361 aaaccccggc acggctgaat gatctgcaac gcaccaccct cgccatcgtc ctcgcgggcg
     3421 gacggggcac gcggcttggg ccgctcacca acaagcgcgt caaaccggcg gtgcacttcg
     3481 gcggcaaata caggatcatc gacttcgcgc tctccaattg tctgaactcc ggcatccgcc
     3541 gcatcgcggt ggtcacgcaa tacaaggcgc actcgttatt gcgccacatg cagcgcggct
     3601 ggggcttcct gcgcggcgaa ttcggcgaat tcatcgatct gtggccggcc cagcagcgtg
     3661 tggaaggcgc gcactggtac cgcggcaccg ccgacgccgt atttcagaat ctcgacatca
     3721 tccgctcgat ccggccgaaa tatgtggtgg tgctggcggg cgaccacatc tacaagatgg
     3781 actacacgcg catgatcgcg gaccacgcgg aaagcggcgc ggattgcacg gtcggctgca
     3841 tcgaggtgcc gcgcatggag gccgtcgcgt tcggcgtgat ggcggtggat gaaaaccgcc
     3901 gcgtgaccgg cttcgtcgaa aaacccgccg atccgcccgc catgccgggc cgccccgatt
     3961 cggcgctcgc gagcatgggc atctatgtgt tcagttccga ttacctgtat tcgctgctcg
     4021 aggagaacat ctcgagcgtc gataccgatc acgacttcgg caaggacatc cttccgcgcg
     4081 tggtcacaca gggcacggca atcgcgcatc cgttcagcat gtcgtgcgtg tcgtcgagcc
     4141 cggacgtgga gccatattgg cgcgacgtcg gcacgatcga cgcctactgg gcagccaacc
     4201 tcgacctcgc ctccaccatt ccgacgctcg acctttacga ccgcaactgg ccgatctgga
     4261 cgtatcagga gcagttgccg cccgccaaat tcgtccgcga catgaaaggg ttgcaaggct
     4321 ccggcaacaa tctgatcgtg tgcggcggct gtgtgatttc gggctcgcag atttcgcgct
     4381 ccgtgttgtc gtcgaacgtc aaagtgagtt cgttctgtaa tatcaatgag gcagtcttat
     4441 tgccgcaggt caccgtcggc gcgagctgcc ggctgcagaa ggtggtggtg gaccgcggct
     4501 gcgccattcc ggacggcacg gtgatcggcg aagatcccgt ggccgacgcc gaacgcttct
     4561 atcgcacgga cgacggcgtc gtgctggtga cgcccgaggc actgcggcag aaggggacgt
     4621 gatccgcgcc ggaacggcaa agcgaaagga ctgaacgggc tggcggagcc gccacccgtc
     4681 ttgccgctcc tgtaagcctt catgtcaccg ttgcgcaaac ctcgcactaa caggaacgag
     4741 accgagccta tgacgattcg cgccctgcac gtcgcaagcg agttgtatcc cctcctcaaa
     4801 acgggcggtc tcgccgacgt cgcgggcgcg ctgccgccgg cgctgagcga gctcggcgcc
     4861 gatgtacggg tgctgctgcc cggcttcccg gcggtggtcg ccggtttgac cgacttgcgg
     4921 ccggtggcgc aactgggccg ccgtttcgat tcgccaggcg tcacgctcga acagggcacg
     4981 ctgccgtcga acggtctgat cgtctatgtg atccgcgctg gtacgctata cgaccggccc
     5041 ggcaacccct atctcaacga cgaacacgtg ccgtacggcg acaacgcgca acgtttcgcc
     5101 ctgctcggct gggtcgccgc gcaactggca cagcacctcg acccggcgtg ggcgccgcag
     5161 atcgtccacg cgcacgactg gcacgccggg ctcgcgccgg cgtatctgaa ggccgctgaa
     5221 cggcagcacg gccgctcttt cgcacgcact gtcttcaccg tgcacaacct ggcgtatcag
     5281 ggcgtttttc cggcgcatct gttcggccag ctggcgctgc cggacgactt cttcagcatg
     5341 cacggcgtcg aattctacgg gcagttgtcg ttcctcaagg cgggcctcta ctacagcgac
     5401 cgtatcacca ccgtcagccc cacctatgcc cgcgaaatcc agacactcgc ccagggcggc
     5461 ggcctcgatg cattgctgcg ccatcgcacg cacgatctga gcggcatcct gaacggcgtc
     5521 gactacgccg tgtggaatcc cgccaccgac gcgctgctcg aagatcacta cacggccagc
     5581 cgtctggccg gcaagctggc ctgcaaggag gcgctgcaga aacgcttcgg cctcgcgcag
     5641 aaaagcgatg cgctgctgtt cggcgtggtg agccggctga ccgagcagaa aggcctcgac
     5701 ctgctgctcg agacggtccc cgaaatcgtc aaacgcggcg gccagctggt ggtgttcggc
     5761 acgggcgatc cggcgctcga acacggtttg aaacgcgtcg cgcatgcgca tccggaatcg
     5821 gtggcggtcg aactgggttt cgacgaaacg ctcgcgcaca caatcgtcgc cggcagcgac
     5881 gtgatcgcgg tgccgtcgcg cttcgagcca tgcgggctga cgcaactgta tgcgctggct
     5941 tatgggtcgc tgccgctcgt gcattgcgtc ggcggcctgg cggacacggt ggtcgacgcg
     6001 tcgctcgaaa atctcgccga cgatctcgcc accggcttcg tgttcgaacg attcgaaccg
     6061 aaaggcctga cggcagcaat ccgccgcgcg tttgcactcc accagcgccg caccgagtgg
     6121 aaagccacgc agcgtcgcgc gatgcgtcag gatttcggct ggggcgcttc ggccgagcgc
     6181 tatctgacgt tgtatcgcga actcgcctga agcgcggctg cgcaaaacct cgacaacggc
     6241 ccactcacgt cgcgctggtt tttacgcaag ccggcgccct aagtgcccct tcgtcccggc
     6301 gcccccgccc ctccgtttga tggttcgacg caacaacacg gggacggatc acctcgcgtg
     6361 tgacaaactc atgtattgtt tcctgactac gcagattggc gtttttcttg catcatctgt
     6421 aacagtcatg ccgcttcggg cgcccgggcc atagccggtt cgttgccgcg gccatccttc
     6481 cgctctcatt gatcgcgcat caccgcctgc gccggccaca ccatgacgaa aaacgttctg
     6541 agcatccagt cacatgtcgt cttcgggcac gccggcaaca gcgcagccgt gtttccgatg
     6601 cgccggctcg gcatcaatgt ctggccgctc aataccgtcc agttctcgaa tcacacgcaa
     6661 tacggccatt ggacgggcgg tgcgatcgag gcctcgcaaa tggaagagct ggtcgagggg
     6721 atcggcgcga tcggcatgct gccgcgctgc gacgccgtgc tgtccggcta tctgggcacc
     6781 cctgagcagg cgcaatcggt gctggaaatc gtcaaggccg tgaaggctgc gaatccacgc
     6841 gcgtggtact tctgcgatcc ggtgatgggc gccgcgagcg gttgcaaggt cgaaccgggc
     6901 atccaggaat ttctcgtgcg gaccatgccg gagatggccg acgcgatggc accgaaccac
     6961 acggagttgc aacgtctggt aggccgcgag atcgatacgc tcgaggaagc cgtgaccgcc
     7021 tgccgcgagg tcatcgcccg cgggccgaaa ctcatgctgg tcaaacatct gctcgatcgt
     7081 aacagccccg ccgaccggtt caacatgctg gtcgtcaccg agcgcgaggc gtggatgggc
     7141 cagcgtccgc tctacccgtt cgcgcggcag ccggtgggcg tcggcgatct gacgagcgcg
     7201 gtgtttgtcg cgcgcacgct gctgggcgat tcgatccgcg cggccttcga acacacgctg
     7261 gccgccgtga acgcggtagt caaggcgacg tggcaggctg ggcgctatga actggaacta
     7321 gtggccgtgc agaacgaaat cgcgcaaccg cgtgaatggt tcgacgcctg ggtggcggag
     7381 acagcgtaag cctataatgc gtgccgcgtg ggcgaggcaa gcacccgccc gcaccgcatt
     7441 tttcaggcat ccggcatccg gcatccgggc tttcgggctt tcgggctttc gggctttcgg
     7501 gtatccggca ccggcatccg gcatccggca ttcagcagac gaggcatcga tggacggtta
     7561 tatccgcagc gagcgggaag aattttttga acagttgtgc atgagcgtcg acgcggacga
     7621 agcgcacgag caggaagcga tcgagtactt cgagaaccag ttcgaccagg ccgacttcga
     7681 tccggcacag tggctcgaca tcgcactcta ctattcgccg gcggtagcgc gcggcatcgt
     7741 cgacatggtg acgcccgacg acaaggcgcg cagcaacatc gccgaagtga tcgccgacaa
     7801 cctcgacatt tcgtacggcg aggacgagtg ccagcagttc gccgagacca tcgaattcgc
     7861 actcaacaat ggcgtgccgg tggacctcga cctggtgctc gacggctgcc agcgcgcgat
     7921 cgacgatctc gacacctggg ccgatgaaga gaccaaggcg ccgctactgc gtctacgtga
     7981 agagttgctg cgccagcagg gcgagcgttg agggcgtttt gcctggcctg cgtttttgtc
     8041 cggctggctt gtcgttttga ttggcgtatt cgcgggtctt gttccgcgaa tgcgtgacta
     8101 gtaaggccgg cgcgcacgtc ggctcgttga acggcttggc cagcattgct ttgctgggcc
     8161 gtcccgctgg tctaccgggg cg
//
LOCUS       DATASH010000906         3648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4704214,
            whole genome shotgun sequence.
ACCESSION   DATASH010000906 DATASH010000000
VERSION     DATASH010000906.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4704214"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VHV99_24185"
     CDS             <1..437
                     /locus_tag="VHV99_24185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX3638519.1"
                     /translation="PSQKVLFSGDLVEYDAACYCGDAQLEQWPATLEALRALTADKLV
                     PGRGPALTTPEDVNKGLDYTKDFVTTLLQQGREAVEQKLDLKAAMAHTRKAMDPKFGH
                     VFIYEHCLPFDVSRAFDEASGITHPRIWTAQRDKEMWDALQA"
     gene            500..2218
                     /locus_tag="VHV99_24190"
     CDS             500..2218
                     /locus_tag="VHV99_24190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013342335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3638520.1"
                     /translation="MSINYQTLSFEYQPCREQSEQSGQGGAEQAPYPVIVVGAGPVGL
                     ATAIDIAQQGVPVVLVDDDCSLSTGSRAICFSKRSLDIFDRLGCGQRMVDKGISWNVG
                     KVFLKDELVYTFNLQPEAGHNRPAFINLQQYYVEGFLLERAREMANLEIRWKSKVVGV
                     QQNGTPGTAQAGVTLTVDTPDGQYPLCGRYVVAADGSRSPMRNLMGLDSKGVTFKDRF
                     LIADVKMEAEFPTERWFWFDPPFHPNQSVLLHRQPDNVWRIDFQLGWDADPVLEKTPE
                     RVIPRVRALLGPDAKFELEWVSVYTFSCLRMERFRHGNVLFAGDSAHGVSPFGARGAN
                     SGVQDAENLAWKLAMVLEGRASDALLDTYASEREFAADENIRHSTRSTDFITPKSPVS
                     RVFRDAVLKLARYHPFARQLTNSGRLSVPAVLRDSPLNTSDGDSFEGLMVPGASCVDA
                     PVQVGGQPAWLLQQLGQQFTGVLFCGSQAIDQATRAALDDLRSGPIPLKLVVVTSGDA
                     QVAAASGANVVHDVDGLAAARYDAKPGTFYLIRPDQHVCARRRQLDASFVVGALKRAL
                     CVEETA"
     gene            2270..2506
                     /locus_tag="VHV99_24195"
     CDS             2270..2506
                     /locus_tag="VHV99_24195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2783 domain-containing protein"
                     /protein_id="HEX3638521.1"
                     /translation="MALNTQPNLTRPDDFYEALIDMHRDLDDAQSQSANAQLILLLAN
                     HIGDHPTLLEAMRHAREGVIDAAVRGSQGHSLAA"
     gene            2583..3278
                     /locus_tag="VHV99_24200"
     CDS             2583..3278
                     /locus_tag="VHV99_24200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HEX3638522.1"
                     /translation="MYSIEPIPLPHDGPSDISLWRLDFAFDTPLEDAAFAPLSDEERA
                     RAQSFRRHEDALRFATVRAALREQLARRLGIAAYAIRFNLDANRRPHLANSDAYDFNV
                     SHAGAHGLIAMSPRRRVGVDIEQYSDKFDWRSIASLTLDASEAAWIEQLGVTQQFAAF
                     YDAWVAKEALVKTTGVGIKRGLQHLTVLPRDSAEVRLRNPIPEDMREIAAQWVAAPQA
                     YAACVAWSITTFP"
     gene            complement(3389..>3648)
                     /locus_tag="VHV99_24205"
     CDS             complement(3389..>3648)
                     /locus_tag="VHV99_24205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012429000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638523.1"
                     /translation="QESRRRARYGSLCNALRYAPADIDLCLLSSDATASAFDLAEHGQ
                     WHVLDLPMSGKIRAAVALAQPVTHVHAFGWPKALVFGKPAA"
BASE COUNT          711 a         1091 c         1174 g          672 t
ORIGIN      
        1 tgccgtcgca gaaggtgttg ttctccggcg acctggtcga atacgacgcc gcctgctatt
       61 gcggcgatgc tcaactggag caatggccgg ccacgctcga agcgctgcgc gcgctgacgg
      121 ccgacaagct cgtgccgggc cgcggccccg ccttgaccac gccggaagac gtcaacaagg
      181 gcctcgatta cacgaaagat ttcgtcacca cgctgctgca gcaaggccgc gaagccgtcg
      241 aacagaagct cgatctgaaa gccgcgatgg cacacacccg caaggcgatg gacccgaagt
      301 tcggccacgt gtttatctac gagcactgcc tgccgttcga cgtttcacgt gccttcgacg
      361 aagcgagcgg catcacgcat ccgcgcatct ggaccgcgca gcgcgacaag gagatgtggg
      421 acgcgctgca agcctgataa cggcatggaa tgccggaaag cgtcaagcgg agcagagcag
      481 agagggacgg agacacaaga tgagcatcaa ctaccagacg ctgtcgttcg agtatcagcc
      541 gtgtcgagaa caaagcgaac aaagcgggca aggcggtgcg gagcaggcgc cctatcccgt
      601 gatcgtggtc ggcgcggggc cggtgggcct cgctaccgca atcgatatcg cgcagcaggg
      661 cgtgccggtc gtcctggtcg acgacgattg ttcgttgtcc accggttcgc gggcgatctg
      721 cttctcgaag cgttcgctcg atatcttcga ccggctcggc tgcgggcagc ggatggtgga
      781 caagggcatc agctggaatg tcggcaaggt gttcctgaaa gatgaactgg tgtacacgtt
      841 caatctgcag cccgaagcgg gccacaaccg gcccgcgttc atcaacctgc agcagtacta
      901 cgtcgaaggc tttctgctcg aacgcgcgcg ggagatggcg aatctcgaga tccgctggaa
      961 gagcaaagtg gtcggcgtgc agcagaacgg cacgccgggc acggctcaag ccggcgtgac
     1021 gctgactgtc gatacgcctg acggtcagta cccattgtgc ggccgctacg tggtggccgc
     1081 cgacggctcg cgcagtccga tgcgcaatct gatgggactc gacagcaagg gcgtcacatt
     1141 caaagaccgc ttcctgatcg ccgacgtgaa gatggaagcg gagttcccga ccgagcggtg
     1201 gttctggttc gatccgccgt tccatccgaa tcaatcggtg ctgttgcatc gtcagccgga
     1261 taacgtgtgg cggatcgatt tccagttagg ctgggacgcc gatccggtgc tggaaaaaac
     1321 gccggaacgt gtgattccgc gcgtgcgtgc attgcttggg cccgacgcga agtttgagct
     1381 ggaatgggtc agcgtctata cattttcgtg tttgcgcatg gagcggttcc ggcacggcaa
     1441 tgtgttgttc gcgggcgatt cggcgcatgg cgtgtcaccg tttggtgcac gcggcgcgaa
     1501 tagcggcgtg caggacgcgg aaaacctcgc atggaaactg gcaatggtgc tcgaaggtcg
     1561 agcgtccgat gccttgctcg acacgtatgc aagcgagcgc gaattcgccg cggacgaaaa
     1621 catccgccac tccacgcgtt caaccgattt catcacgccg aagagtccgg tcagccgcgt
     1681 gtttcgcgat gctgtgctga agctcgcgcg gtatcacccg tttgcgcggc agttgacgaa
     1741 tagcggccgc ctgtcggtgc cggcggtgct gcgtgattcc ccgctgaata catcggacgg
     1801 cgacagcttc gaaggtttga tggtgccggg cgcatcgtgc gtcgatgcgc cggttcaggt
     1861 gggcgggcaa ccggcatggc tgcttcagca actcggccag caattcacgg gcgtgttgtt
     1921 ctgcggctcg caagctatcg atcaggcgac gcgagctgcg ctggatgatt tgcgcagcgg
     1981 tccgattccg ctgaagcttg tcgtggtaac gagtggcgat gcacaggtcg ctgcggcttc
     2041 gggtgcaaat gtggtgcacg acgtcgatgg tcttgcagcc gcgcgttatg acgccaaacc
     2101 cggcacgttc tatttgattc gcccggacca gcatgtctgc gcgcgccggc ggcaactcga
     2161 tgcgagcttt gtggtggggg cgttgaagcg cgcgttgtgc gtggaagaga cggcctgagg
     2221 atatcggcac atgcagcgca tccgcacaag aaaacagcag gagacagcca tggcattgaa
     2281 cacgcaaccc aatctcacgc gccccgacga tttctacgag gcgttgatcg acatgcatcg
     2341 cgatctggac gatgcacaga gtcagtcggc caatgcgcag ctgatcctgt tgctcgccaa
     2401 ccatatcggc gatcacccga cgctgctcga agcgatgcgg cacgcacgtg agggcgtgat
     2461 cgacgctgcg gtgcgaggct cacaggggca ttcgctggct gcttgaatca acaggagcgt
     2521 cagatcgggt acagtcgcgc atattccctt gcgactgtac gttcaacatc cgaaccttcg
     2581 cgatgtattc gatcgaaccg attccattgc cgcacgacgg cccgtccgac atttcgttgt
     2641 ggcggctcga tttcgcgttc gacacgccgt tggaggatgc ggccttcgcg ccactcagcg
     2701 acgaagaacg cgcgagagcc caaagctttc gccgccacga agacgcgctg cgttttgcaa
     2761 cggtgcgcgc ggcactgcgc gagcaactcg cgcggcgtct cggcatcgcg gcatacgcca
     2821 tccggttcaa tctcgatgcg aaccgccgtc ctcatctcgc caattccgac gcatacgatt
     2881 tcaacgtctc ccatgcgggc gcgcatggac tgatcgcgat gtcgccgcgc cggcgcgtcg
     2941 gcgtcgatat cgagcagtac agcgataagt tcgattggcg ttcgatcgca tcattgacgc
     3001 tcgatgccag cgaggcggca tggatcgagc agctaggcgt cacgcagcag ttcgccgcgt
     3061 tttacgacgc atgggtcgcc aaggaagcgc tcgtcaaaac gacgggtgtg ggcatcaagc
     3121 gcggcttgca gcatctgacg gtgctgccgc gtgacagtgc cgaggtgagg ctgcgcaatc
     3181 cgattccgga ggacatgcgc gagattgcag cgcagtgggt cgcggcaccg caggcttatg
     3241 ccgcatgcgt ggcgtggtcg atcaccacat tcccttgaga catcctgggt cgaacgcgag
     3301 ccgcgttgct cgatccgccc aacacgtggc cgactgcatc gccgcgcagc gcgtcaatcc
     3361 atccgattcg caataacggc acgccacact atgcagcggg cttgccaaac accaacgcct
     3421 tcggccatcc aaaggcatgc acgtgcgtga ccggctgcgc gagtgcaacc gccgcgcgaa
     3481 tcttgcccga catcggcaga tcgagcacat gccactggcc atgttcggca agatcgaatg
     3541 cgctggctgt ggcatcgctc gacagcagac acagatcgat atcggcgggc gcgtagcgca
     3601 acgcattgca taggctgccg taacgcgcgc gtcggcgtga ctcttgcg
//
LOCUS       DATASH010000907        12496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6086762,
            whole genome shotgun sequence.
ACCESSION   DATASH010000907 DATASH010000000
VERSION     DATASH010000907.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 12496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12496
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6086762"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /gene="nodI"
                     /locus_tag="VHV99_24210"
     CDS             complement(<1..819)
                     /gene="nodI"
                     /locus_tag="VHV99_24210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004538328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation factor ABC transporter ATP-binding
                     protein NodI"
                     /protein_id="HEX3638524.1"
                     /translation="MSEAAIEFHQVRKSYGEKIVVDGLSFHVNAGECFGLLGPNGAGK
                     TTTLRMLLGIAAPDGGTIRLCGEPIPGRARVARARVGVVPQFDNLDPDFTVRENLLVF
                     GRYFGLSAAQCRAMVPSLLEFARLENKADARVSELSGGMKRRLTLARALVNDPDVLIM
                     DEPTTGLDPQARHLIWERLRSLLTRGKTILLTTHFMEEAERLCHRLCVIEEGRKIAEG
                     APSELIASEIGCDVIEIFGPDPVALRDELAPLVERTEISGETLFCYVNDAQPVHA"
     gene            1123..1593
                     /locus_tag="VHV99_24215"
     CDS             1123..1593
                     /locus_tag="VHV99_24215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX3638525.1"
                     /translation="MASYQKILLCYDGSREGRKALRCGADLALDLKAETHLLSVVDMR
                     SSIAQSAGLLTDVACGSFEKTARDILQEGVDWLTERGVTAQGHFAFGHPIDEIANLAN
                     ELHVDLVVVGHRCRTGLSRWWMGAGNTPLLDRVSCSILVACSSAQEQEQQAAAA"
     gene            1615..1730
                     /locus_tag="VHV99_24220"
                     /pseudo
     CDS             1615..1730
                     /locus_tag="VHV99_24220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018418191.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
     gene            complement(1887..2561)
                     /locus_tag="VHV99_24225"
     CDS             complement(1887..2561)
                     /locus_tag="VHV99_24225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2939 domain-containing protein"
                     /protein_id="HEX3638526.1"
                     /translation="MAVSTRTKRNATRPLIVTLIVIAVIAALGFGYASPYMALNNLKR
                     AADARDAQTVNEYVDFPALRESLKQQVTGLLSRRLDAHGSGNPLAAIGAMIGVALIGP
                     LVDAYATPDGVAALLNGIPPRGDPGERPPAPPAAGNSPDAAAPASVPAAHAAGNGVNS
                     AANSGNSGENSATPPQPPQTTAGYRSLNEFVVTYQHGAGDARYSAIFRREGLFTWKLT
                     AVNLNE"
     gene            complement(2732..3076)
                     /locus_tag="VHV99_24230"
     CDS             complement(2732..3076)
                     /locus_tag="VHV99_24230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018432112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638527.1"
                     /translation="MERLARLLPFRQFGRLRHLRKLVPCSLIEPSSASAPSLFDAPAS
                     ASSLPSSLPAFARVSLNSGLNTAEPARRAPVRVYHGPSRLIMVGTVDAVCRMIDRCIA
                     DEANVHGAVFEA"
     gene            complement(3213..3863)
                     /gene="lexA"
                     /locus_tag="VHV99_24235"
     CDS             complement(3213..3863)
                     /gene="lexA"
                     /locus_tag="VHV99_24235"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009912524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEX3638528.1"
                     /translation="MTKLTARQQQVFDLVRRAIERTGFPPTRAEIAAELGFSSANSAE
                     EHLRALARKGVIELAAGASRGIRLLTGAEDSPYQFTLPHASIMQLSLPLIGRVAAGSP
                     ILAQEHISQHYACDPALFSSKPDYLLKVRGLSMRDAGIFDGDLLAVQKKSEAKDGQII
                     IARLGDDVTVKRLKRRPNGIELIAENPDYDNIFVETGSAEFALEGIAVGLIRPGEF"
     gene            4180..5220
                     /locus_tag="VHV99_24240"
     CDS             4180..5220
                     /locus_tag="VHV99_24240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006759460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX3638529.1"
                     /translation="MNHQGTGLAGKTKKLIAALAFGAAAALGVMAQAHAADTTLLNVS
                     YDPTRELYQDVNQAFGKEWKAKTGETVTFKQSHGGSGAQARSVLDGLQADVVTLALAY
                     DIDALASKGLVDKGWQKRLPDNASPYTSTIVFLVRKGNPKHIKDWDDLIKPGVSIVTP
                     NPKTSGGARWNYLAAWAYAEHLPGGNDQKAKDFVGKLYKNAGVLDSGARGATTSFVQR
                     GIGDVLIAWENEAFLSLKDFGPDKFEIVVPSVSILAEPPVAVVDKVVDRHGTRKVAEA
                     YLNFLYSEQGQEIAARNFYRPRSNKVPAELTAKFPKLKLYTIDDSFGGWANAQKTHFA
                     DGGVFDSIYSPQ"
     gene            5378..6280
                     /gene="cysT"
                     /locus_tag="VHV99_24245"
     CDS             5378..6280
                     /gene="cysT"
                     /locus_tag="VHV99_24245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEX3638530.1"
                     /translation="MTTLTFRKPSALPGFGLTLGITVAYLSLVVLIPLAAAFLKTATL
                     DWPQFVRAVSSPRVLASYRLTFFSALGGALINAVFGFLVAWVLVRYTFPFKRIVDAVV
                     DLPFALPTSVAGISLAAVYAGNGWIGQFLEPLGIKIAFTPAGVLVALTFIGLPFVVRT
                     VQPVLEEFEREQEEAAACLGASRWLTFRRVVLPAVFPALLTGFALAFARALGEYGSVI
                     FIAGNVPMKSEITSLLIITKLEQYDYAGATAIAVVMLVVSFLMLLFINTLQWYLQRRT
                     SRGGADPAPAAASVAAIGVGGGAQ"
     gene            6277..7299
                     /gene="cysW"
                     /locus_tag="VHV99_24250"
     CDS             6277..7299
                     /gene="cysW"
                     /locus_tag="VHV99_24250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HEX3638531.1"
                     /translation="MSRDSVKNAATGAVAVAPRAPLNVARRPDPVTEPPIVRWVLTGV
                     ALLFLALFLVVPLVAVFYQALAKGVGFYLESLADPDALSAIKLTVITAAIAVPLNLVF
                     GLAASWCIAKFEFRGKALLTTLIDLPFSVSPVISGLLYVLMFGAQGWFGPWLLDHNVQ
                     IIFAVPGIVLATIFVTFPFVARELIPLMQAQGNDEEEAAHVLGASGWQIFRRVTLPNV
                     KWGLLYGVILCNARAMGEFGAVSVVSGHIRGQTDTMPLHVEILYNEYNFSAAFAVASV
                     LALLALVTLGLKLLAERHMSAELSAARDVPAYAGPVTPTSASQSVNAPHVSMNAVKHP
                     LKQGEL"
     gene            7299..8357
                     /locus_tag="VHV99_24255"
     CDS             7299..8357
                     /locus_tag="VHV99_24255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HEX3638532.1"
                     /translation="MGITVRNLQKRFGDFVALDNVSLDFPPGELVALLGPSGCGKTTL
                     LRVIAGLEYADGGQVVLQGQDVATVGAREREVGFVFQHYALFRHMTVFENVAFGLRVK
                     PRKERPSEAVIREKVHELLKLVQLDWLAQRYPSELSGGQRQRIALARALAVEPKVLLL
                     DEPFGALDAKVRKELRSWLRRLHDDLHISTIFVTHDQEEALEVADRIVVLNRGHVEQV
                     GSPQDVYDHPQTSFVYEFLGAANRLHGNVDASGFVVDGAALPVAIKADFKGPAFAYVR
                     PHDLQLYPQASGHREGIVVDVRRVVTLGGSVRVELAGREGNVLEAELDRESWRDLQLA
                     IGDGVTAVPRALRVFPAQ"
     gene            8465..9391
                     /locus_tag="VHV99_24260"
     CDS             8465..9391
                     /locus_tag="VHV99_24260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011884743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HEX3638533.1"
                     /translation="MNFQQLRFVREAVRQNMNLTEVANVLYTSQSGVSKQIKDLEDEL
                     GVDIFIRRGKRLTGLTEPGKAVHLLIERMLLDAENLRRVARQYADQDSGHLVVATTHT
                     QARYALPKVIRQFTEVFPKVHLALRQGSPQQIAQMIINGEADIGISTEALDRFPDIVT
                     FPCYSWHHVVVVPKDHPLVGRENLTLDEIAEFPIVTYDQDFTGRSHIDQAFAKAGALP
                     DVVLTAIDADVIKTYVELGMGIGVVAAMAYDPKRDTELVALDTQHLFEASTTRVGLRK
                     GAFLRAYAYRLIEMFAPQLNEAEIAAQLREAV"
     gene            9426..10469
                     /locus_tag="VHV99_24265"
     CDS             9426..10469
                     /locus_tag="VHV99_24265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEX3638534.1"
                     /translation="MNTPTSSSATSSVAGATPRLPRIAVLATGGTIAGAAADATNTSG
                     YQAGVVGVDQLLAVVPALSTVARIAAEQIASVDSKDMAMALWTTLAQRINTLLAEDDI
                     DGVVVTHGTDTLEETAWLLHLTIKSDKPVVLTAAMRPASALSADGPLNLLNAVTVAAN
                     AGARGQGVLVAFNNKIHSARDVVKTSTYAVDAFQSPEIGALGWVQDGRVEFQRSVVRP
                     HTRATEFVIGAKWPHVEVVASYAGVTRIAVDALVAAGVRGIVVAGTGNGSIHASMQQA
                     LADAASQGVAVVRSSRVGSGHVMRNGAAADDALGFVSAGSLNPYKARVLLMLALAAGG
                     TGPVALQKTFDTY"
     gene            complement(10630..11031)
                     /locus_tag="VHV99_24270"
     CDS             complement(10630..11031)
                     /locus_tag="VHV99_24270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3597 domain-containing protein"
                     /protein_id="HEX3638535.1"
                     /translation="MSIFGDIVNKLFGKAKPDQPAPAVEPTPDPAAAQAAAPAATAAP
                     APLADVDVAVVMDQFVSESGQTLNWRTSIVDTLKALGVDSSLEHRKQLAKELKYSGDT
                     NDSASMNIWLHKQVMQALAANGGKLPPDLAA"
     gene            complement(11600..>12496)
                     /locus_tag="VHV99_24275"
     CDS             complement(11600..>12496)
                     /locus_tag="VHV99_24275"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0019580 - galactarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HEX3638536.1"
                     /translation="GFIGLGIMGAHMARNLIKGGHTLFVNGAYPVPEDLSKSASVVAN
                     STAVAQAADIVIIMVPDTPDVANVLFADDGVAAGLAQGKLVIDMSSISPLDTQAFAKK
                     INALGVDYLDAPVSGGEVGAREASLTIMVGGPEKAFATARPLFELMGKNISLIGDNGA
                     GQTCKVANQIIVALNIEAVAEALLFASRSGADPERVRKALMGGFASSRILEVHGERMT
                     RRKFDPGFRIELHQKDLNLALDGARKLGIALPHTASAQQLFSVCAANGGKAWDHSAMV
                     RALEIMANFEVAQAPGSEAKAA"
BASE COUNT         2265 a         3790 c         4046 g         2395 t
ORIGIN      
        1 ggcatgcacc ggttgggcat cgttcacata acagaacagc gtctcgccgc tgatctcggt
       61 gcgctcgaca agcggcgcca gttcatcgcg caacgcgacg ggatcgggtc cgaagatttc
      121 gatcacatcg caaccgatct cggatgcgat cagctcgctc ggcgcacctt cggcgatctt
      181 gcgtccttcc tcgatcacgc agaggcggtg gcacagccgt tcggcctcct ccatgaagtg
      241 cgtggtcagc agaatggtct tgccgcgtgt gagcagagag cgcagccgct cccagatcag
      301 atggcgcgcc tgcggatcga gaccggtggt cggctcgtcc atgatcagca cgtcggggtc
      361 gttgacgagc gcgcgagcca gcgtgaggcg ccgcttcatg ccgcccgaca gctcactcac
      421 gcgcgcgtcc gccttgttct cgaggcgcgc gaattcgagc agcgaaggca ccatcgcgcg
      481 gcactgcgcg gcgctcagac cgaagtagcg tccgaacacc agcaggttct cgcggaccgt
      541 gaaatcagga tcgagattgt cgaactgcgg taccacgccg acccgcgccc gcgcaacgcg
      601 cgcgcggccg ggaatcggct cgccgcacaa gcggatcgtg ccgccatccg gcgcggcgat
      661 gccgagcagc atgcgcaacg tcgtggtctt gcctgcgccg ttcggaccga gcaggccgaa
      721 acattcgcca gcgttcacat gaaacgacag cccgtcgacg actatttttt cgccgtagct
      781 ttttctaacc tggtgaaatt caatggccgc ttcagacatg gatcgatcga ggctccgcaa
      841 aagacgcaaa tggccgccaa cgccgccagg acccctgagg cccctgaggc cgcccattct
      901 atggcatcgg caacgcagtg ttgaccgccg ccggctgcgc ggaatggcgt ttcttgcgct
      961 gcgacaatag gtccatcggt gattcaaacc cggcccagac aggcccacga cgcagcgcgc
     1021 acctgtttcg ggcgcagtta gcgttttcca tcccttgcaa cctgaattgc ggcgcaccat
     1081 gattgattca gaccgcgccc ctcgcgggat gggagagaaa tcatggcgag ctaccagaaa
     1141 atcctgttgt gctacgacgg ctcgcgcgaa ggccgcaaag cgttacgttg cggcgccgac
     1201 ctggcgttgg atctgaaggc ggaaacgcac ttgttgtcgg tggtcgacat gcgctcgagc
     1261 attgcgcaga gcgcgggcct gctgaccgat gtagcgtgcg gaagcttcga aaaaacagcc
     1321 cgcgatattt tgcaggaagg ggtggactgg ctcaccgaac gcggcgtgac cgcgcaaggg
     1381 cattttgcgt ttggccatcc aatcgacgag atcgccaacc ttgccaacga gttgcatgtc
     1441 gatctcgtgg tggtcggcca ccgttgccgc accggtctgt cacgatggtg gatgggcgca
     1501 ggcaatacgc cgctgcttga ccgggtctcg tgcagcattc tggtggcgtg ttcttccgcg
     1561 caggagcagg agcagcaagc agcggctgcc tgacgcacgt ggggcggggg gcgtggactg
     1621 tcacgatggt ggatgggcgt gggaataccc cgctgctcaa ccggatctct tgctgcattc
     1681 tgatggcgtg ttcttccgcg caggagcagc agcaagcggc ggctgcctga cgcacgtcgg
     1741 ggcggagggc gtgtgctggg ctcgacgggc cacatggact gaattggcta tgtggtccct
     1801 ggtgccaggg agccgaacgt tcgctcgccc agggcgaccg ttcggccaaa cggtgattcg
     1861 ctcaagcaat agcgctcgcc aaacgatcac tcgttcaggt tgaccgcggt gagcttccac
     1921 gtgaacaatc cttcgcgccg aaagatcgct gaataacgcg catcgccagc accatgttgg
     1981 taggtgacga cgaactcgtt caggctgcga tagcctgcgg ttgtctgcgg cggttgcggt
     2041 ggcgttgcac tgttctcgcc gctattgccg ctattggccg cactatttac accattgccc
     2101 gcggcgtggg ctgcaggcac cgatgcaggc gccgcggcgt ccggcgaatt tccagcggca
     2161 ggcggcgccg gtggccgctc acccggatcg ccgcgcggcg gtatgccgtt cagcaaagcc
     2221 gcaacgccgt cgggtgttgc ataagcatcg accagtggac cgatcaaggc cacgccgatc
     2281 attgcgccaa tggccgccaa cggattgccg ctgccatgcg cgtcgagccg ccgcgataac
     2341 aagccggtga cctgctgctt caggctctcg cgcaaggcgg ggaagtcgac gtattcattg
     2401 acggtttgcg catcacgcgc atccgccgcg cgtttgaggt tattcagcgc catgtagggc
     2461 gacgcgtaac cgaaccccaa tgcggcgatc accgcaatca caatcaatgt gacgattaga
     2521 ggccgggttg cgttgcgttt ggtgcgtgtt gaaactgcca tcgagcggga ttctccatca
     2581 ggtgtgttct aacgtggacc gcggccacgc gaaaacgatc ccagacgatg tgctgtgaag
     2641 gcggtacggc agcccaaaaa acttctcgcg gcatagcctg catatccgcg ccgcacaacc
     2701 gcattggagc ggagtgcccg cccagtgccg cttatgcctc gaacaccgcg ccgtgcacat
     2761 tggcctcgtc ggcgatacag cgatcgatca tgcggcatac ggcgtcgacc gtgccgacca
     2821 tgatcagccg cgacggcccg tgatagaccc gtaccggcgc gcgacgcgcc ggttcagccg
     2881 tattcagccc tgaattcaac gacacgcgag caaacgccgg caatgaggac ggcaagctcg
     2941 atgcgctggc gggcgcgtcg aacagcgacg gagcactggc cgaggacggc tcgatcagtg
     3001 agcagggcac cagcttgcgc agatggcgca aacgaccgaa ctggcgaaaa ggcagcaggc
     3061 gggcaaggcg ttccatgagt ctctccaggc gattcaacgt tggggcagcg ttcgggcagc
     3121 tttgaggcat tttcgggggg cggctggggg ccgctttgag gcagccctca ggcagctttt
     3181 gagccggctt cgggcggcgc aggagcgagt ggttagaact cgcccggacg gatcagcccg
     3241 acagcgatac cttcgagcgc gaattccgca ctgccggttt caacgaagat gttgtcgtaa
     3301 tcggggttct cggcaatcag ctcgataccg ttcggccggc gcttcagccg cttgaccgtc
     3361 acgtcgtcgc caagccgcgc gatgatgatc tggccgtctt tggcttcgct ttttttctgc
     3421 accgcaagca agtcaccgtc gaagatgccc gcgtcgcgca tggacaggcc gcgcaccttc
     3481 agcaggtagt ccggcttgct cgagaacagc gccgggtcgc atgcgtagtg ctgcgagata
     3541 tgttcctgcg cgaggatcgg gctacccgcg gccacgcggc cgatcagcgg caacgacagt
     3601 tgcatgatgc tggcgtgcgg cagcgtgaac tggtacggcg aatcttccgc gcccgttagc
     3661 aggcgaatgc cgcgcgatgc gccggccgcg agttcgatca cacccttgcg agccagcgcg
     3721 cgcaaatgtt cttctgccga attggccgag ctgaaaccca gctcggcggc gatctccgcg
     3781 cgggtgggcg gaaagccggt gcgttcgatg gcccggcgga ccagatcgaa aacctgctgc
     3841 tgtcgtgcgg tgagtttggt catggcgcta ctgtatggat gaacaggtag ctgtattttt
     3901 atacagtatt ccgcgcaatt caagctttac tttaagttcg tggcgcgggg tggcgttcga
     3961 gcgtcggttt ccgacgtctc aacagccgtt ttcgtgccgc aacgccttat ccagccgcca
     4021 ttaccacttt tgcgtattaa agaatgagga aacattattt ttaatgatga aagcccttcg
     4081 atagactggc ctcccagttg gcgctcatgt gcacgtgacc gcgacgcaaa acaacaccac
     4141 acgctgtttc agcggaccac cacgcggaga aagttggcaa tgaatcatca aggcacgggg
     4201 ttggcaggca agaccaaaaa actcatcgcg gcgctcgcat tcggcgcggc agcggcgctc
     4261 ggcgtcatgg cgcaggcaca tgcagccgac acgacgctgc tgaacgtgtc gtacgacccg
     4321 acgcgtgagt tgtaccagga cgtcaaccag gcattcggca aggaatggaa ggcgaaaacg
     4381 ggcgagacgg tcaccttcaa gcagtcgcac ggcggctcgg gtgcgcaagc acgctcggtg
     4441 ctggacggtc tgcaggcgga cgtggtgacg ctggcgctcg cctacgacat cgacgcactg
     4501 gctagcaagg gtctggtcga caagggctgg caaaagcgtt tgccggacaa cgcctcgccg
     4561 tacacgtcga cgatcgtgtt cctggtgcgc aagggcaacc cgaagcacat caaggactgg
     4621 gacgatctga tcaagccggg tgtgtcgatc gtcacgccga atccgaagac ctcgggcggc
     4681 gcgcgctgga actacctggc cgcatgggcc tatgctgaac atctgccggg cggcaacgac
     4741 cagaaggcca aggacttcgt cggcaagctc tacaagaacg ccggcgtgct ggattcgggc
     4801 gcgcgtggcg cgaccaccag cttcgtgcaa cgcggtatcg gcgacgtgct gatcgcgtgg
     4861 gaaaacgaag cgttcctctc gctgaaggac tttggtccgg acaagtttga aatcgtcgtg
     4921 ccgtcggtga gcattctggc cgagccgccg gttgcggtgg tggacaaggt ggtcgacagg
     4981 cacggcacgc gcaaggtggc cgaagcgtat ctgaacttcc tgtacagcga gcaaggtcag
     5041 gaaatcgccg cgcggaactt ctaccgtccg cgttcgaaca aggtgccggc tgagctgacg
     5101 gccaagtttc cgaagctgaa gctgtacacg attgacgatt cgttcggcgg ctgggcgaac
     5161 gcgcagaaga cgcactttgc cgatggcggc gtgttcgatt cgatctactc gccgcagtaa
     5221 ggcgcgctta gccgcggtta gcaatagaca gctggatccg cgccgttcgg cgcaacgtat
     5281 cacccagcca cgaagcgcgc ccaccgggcg cgcttttttg gcgagccagg cggccgaaaa
     5341 aagagccgcc ggcctcaacc ctgaaagagt actcagcatg acgacgttga ccttccgaaa
     5401 accgagcgcg ttgcccggtt ttggcctgac acttggcatc acggtggctt atctgagcct
     5461 cgtggtgctg attccgcttg cggcggcctt tctcaagacc gcgacgctcg actggcctca
     5521 gttcgtgcgc gcggtgagct cgccgcgcgt gctcgcgtcg tatcgcctca cgtttttctc
     5581 ggccctcggc ggcgcgctga tcaacgcggt gttcggcttt ctggtcgcgt gggtgctggt
     5641 gcgctatacg tttccgttca aacgcatcgt cgacgcagtg gtcgatctgc cgtttgcttt
     5701 gccgacctcc gtggccggca tttcgctcgc ggccgtgtat gcgggcaacg gctggatcgg
     5761 gcagtttctc gaaccgctcg gcatcaagat cgcgtttacg ccggccggtg tgctggtcgc
     5821 gctgaccttc attggtttgc cgttcgtcgt gcggaccgtg cagccggtgc tcgaagagtt
     5881 cgaacgcgaa caggaagaag ccgccgcgtg cctcggcgcg tcgcgctggc tgacgtttcg
     5941 ccgcgtggtg ttgccggcgg tgttccccgc gttgctgacc ggcttcgcgc tggcgttcgc
     6001 acgggcgctc ggcgaatacg gctcggtgat tttcatcgcc ggtaacgtgc cgatgaaatc
     6061 ggaaatcacg tcgctgctga tcatcaccaa gctggagcaa tacgactatg ccggcgccac
     6121 ggccatcgcg gtcgtgatgc tggtggtgtc gttcctgatg ctgttgttca tcaatacgct
     6181 gcagtggtat ctgcagcgcc gcacgagtcg tggcggagcg gatcccgcgc cggccgccgc
     6241 gagtgtggcg gcgatcggtg tcggcggagg tgcgcaatga gccgcgattc cgtcaagaac
     6301 gccgcgactg gcgcggttgc cgtggcgccg cgtgcgcccc tcaatgtcgc gcgccggccc
     6361 gatccggtca ccgagccgcc catcgtgcgc tgggttctga ctggcgtcgc gttgctgttt
     6421 ctcgcgctgt tcctcgtcgt gccgctcgtc gccgtgttct atcaggcgct cgccaagggc
     6481 gtcgggttct atctggaatc gttggccgat ccggacgcat tgtcggccat caagctcacc
     6541 gtgatcactg cggcgattgc cgtgccgctg aatctggtgt tcggtcttgc ggcgtcatgg
     6601 tgtatcgcca agttcgagtt ccgcggcaag gcattgctga ccacgttgat cgatctgccg
     6661 ttctcggtct cgccggtgat ctccggtttg ctctacgtgc tgatgttcgg cgcgcaaggc
     6721 tggttcggtc cgtggttgct agaccacaac gtgcagatca ttttcgcggt gccgggcatc
     6781 gtgctggcga cgatcttcgt cacgttcccg ttcgtcgcgc gtgaactgat tccgctgatg
     6841 caggcgcaag gcaacgacga agaagaagcc gcgcacgtgc tcggcgcgtc gggctggcag
     6901 atcttccgcc gcgtcacgct gcccaacgtc aaatggggcc tgctgtacgg cgtgattctg
     6961 tgtaacgcac gggcaatggg cgagttcggc gcggtgtcgg tggtgtccgg ccacattcgt
     7021 ggccagaccg acacgatgcc gctgcacgtc gaaattctct ataacgaata caacttctcg
     7081 gcggcgttcg ccgtggcgtc ggtgctggcc ttgctcgcac tcgtcacgct cggtctgaag
     7141 ctgctcgccg agcgccatat gtcggcggaa ctgtcggccg cgcgcgatgt gcccgcgtat
     7201 gccgggcctg tcacgccgac ttccgcttca cagtcagtca acgcaccgca tgtgtcgatg
     7261 aatgcagtca agcacccgct caagcaagga gagctgtaat gggtatcacc gttcgtaacc
     7321 tgcaaaagcg cttcggcgat ttcgtcgcgc tcgataacgt ttcgctagac tttccgccag
     7381 gcgaactggt tgcgctgctc gggccatcgg gttgtggcaa gaccaccttg ctgcgtgtga
     7441 tcgccggact tgaatacgcg gacggcggcc aggtcgtgct gcaaggtcag gacgtcgcga
     7501 cggtgggcgc gcgtgaacgt gaagtcggct tcgtgttcca gcattacgcg ctgttccgtc
     7561 acatgacggt gttcgagaac gtcgcgttcg gcttgcgcgt gaagccgcgc aaggagcggc
     7621 cttcggaagc ggtgattcgc gagaaggtgc atgaactgct gaagctcgtg caactcgact
     7681 ggcttgcgca acgctatccg tcggaactgt cgggcggcca gcggcagcgg attgcgctgg
     7741 cgcgcgcact ggctgtcgaa cccaaggtgt tgttgctcga cgaaccgttc ggcgcgctgg
     7801 acgcgaaggt gcgcaaggaa ttgcgcagct ggctgcgtcg tttgcatgac gacctgcata
     7861 tctcgacgat tttcgtcacg cacgaccagg aagaagcgct tgaagtagcc gaccggatcg
     7921 tcgtgttgaa tcgcggacat gtggagcagg tgggcagtcc gcaggacgtg tacgatcatc
     7981 cgcaaacttc gttcgtctac gagtttctcg gcgcggcgaa ccgtctgcat ggcaacgtgg
     8041 atgcgagcgg cttcgtggtc gacggcgcgg cgctgccggt cgcgatcaag gctgacttca
     8101 aggggccggc cttcgcctat gtacgtccgc acgatctgca gttgtacccg caagcatcgg
     8161 gccaccgcga gggcattgtg gtcgacgtgc ggcgcgtggt gacgctcggc ggctcggtgc
     8221 gtgtggaact ggcgggccgc gagggcaatg tgctcgaggc ggaacttgat cgtgaatcgt
     8281 ggcgcgatct gcaattggca atcggcgatg gtgtcactgc cgtgccgcga gcgttacgcg
     8341 tatttcccgc gcaatgagcg gcgccatgag cgactccgta agcgacagcg taagcaccgc
     8401 aacaacactc ggcgtttgaa tcgaactcta ataaattaac tctggcccaa ccggagacat
     8461 acagatgaat ttccagcaat tgcgctttgt gcgcgaggcc gtgcgtcaga acatgaatct
     8521 gactgaggtg gcgaacgtgt tgtacacgtc gcagtcgggc gtatcgaaac agatcaagga
     8581 tctggaagac gaactcggcg tcgacatttt cattcgacgc ggcaagcgtc tgacagggct
     8641 taccgagccg ggcaaggcgg tgcatctgtt gatcgagcgg atgctgctcg atgcggagaa
     8701 tctgcgccgc gtcgcgcgtc aatacgcgga tcaggacagc ggccacctcg tggtggcgac
     8761 gacacacacg caggcgcgtt acgcgctgcc gaaggtgatt cgtcaattca ccgaggtgtt
     8821 cccgaaggtg catctggcgc tgcgccaggg cagcccgcaa cagatcgcgc agatgatcat
     8881 caacggcgaa gcggatatcg gcatttcgac cgaagcgctc gaccgcttcc cggatatcgt
     8941 cacgttccca tgctattcgt ggcatcacgt cgtggtggtg ccgaaggacc atccgctggt
     9001 gggccgcgag aatctgacgc tcgacgagat cgccgaattc ccgatcgtca cgtacgacca
     9061 ggacttcact ggccgctcgc acatcgacca ggcgttcgcg aaagcaggcg ccttgcccga
     9121 cgtcgtgttg accgcgatcg atgccgacgt gatcaagaca tatgtcgaac tcggcatggg
     9181 catcggcgtg gtcgccgcca tggcttacga cccgaagcgc gatacggaac tggtcgcgct
     9241 cgatacgcag catctgttcg aggcgagcac gacgcgggtc ggcttgcgca agggcgcgtt
     9301 cctgcgtgcg tacgcgtacc ggctgatcga aatgttcgcg ccgcagttga acgaagcgga
     9361 aatcgcggcg cagttgcgcg aggcggtttg aggcggcgcg tggcgcatcg cttacaatcg
     9421 ccgccatgaa tactccgact tcctcttccg cgacgtcttc cgttgcaggc gctacgcccc
     9481 ggctgccgcg catcgccgtg ctggcaaccg gcggcacgat cgccggagcg gccgcggacg
     9541 ccaccaacac gtccggctac caggccggtg tggtcggcgt cgatcaattg ctggcggtgg
     9601 tgccggctct gtccacggtc gcgcggatcg cggccgaaca gattgccagc gtcgacagca
     9661 aagacatggc aatggcgcta tggaccacgc tcgcgcaacg catcaatacg ctgctggccg
     9721 aagatgacat cgacggcgtg gtggtcacgc atggcaccga tacactcgaa gaaaccgcct
     9781 ggctgctgca tctgacgatc aagtcggaca agccggtcgt gctgaccgcg gcaatgcgcc
     9841 cggcatcggc gctctccgcc gatggcccat tgaatctgct gaacgccgtg acggttgcgg
     9901 cgaacgcggg ggcgcgcggg cagggcgtgc tggtggcctt caacaacaag attcacagcg
     9961 cacgcgacgt ggtcaagacg agcacgtatg ccgtcgacgc gttccagtcg ccggagatcg
    10021 gcgcgcttgg ctgggtgcag gacggccgcg tcgaattcca gcgcagcgtg gtgcgcccgc
    10081 atacgcgcgc gacggaattc gtgatcggcg cgaagtggcc gcacgtggaa gttgtcgcga
    10141 gctatgcagg cgtcacgcgg atcgccgtgg atgcgttggt ggcagccggc gtgcgcggca
    10201 tcgtcgtggc gggtacgggc aatggctcga ttcacgcctc catgcaacag gcattggccg
    10261 atgcggcctc gcaaggcgtg gcggtggtgc gttcctcacg cgtaggctca ggccacgtga
    10321 tgcgtaatgg cgcggccgcg gacgatgcgc tcggtttcgt cagcgcgggc tcgctgaatc
    10381 cgtacaaggc gcgggtgctg ctgatgctcg cactggccgc gggcggcacg gggccggttg
    10441 cgttgcagaa gactttcgat acgtactgat tgatttagcg cggatggccc ggttcaaagt
    10501 cagaaccgtg tcccgcacat gaagcttaaa taacaacgcg gcgcccaggt catcgacctg
    10561 agcgccgcgt tgtcgttttc agcttaagtt ttgtcgctat cgccgcaacc aggcgacgcc
    10621 ggcaagccgt caggccgcca gatccggagg caacttcccg ccgtttgccg ccagtgcttg
    10681 catcacctgt ttgtgcagcc agatgttcat gcttgccgaa tcgttcgtgt cgccactgta
    10741 cttcagctcc ttggcgagct gcttgcgatg ctcgaggctg ctgtcgacgc cgagcgcttt
    10801 cagcgtgtcg acgatcgacg tgcgccagtt caacgtttgc ccgctctcac tcacgaactg
    10861 gtccatgacg acagcgacgt cgacgtcggc cagcggcgcg ggtgccgctg tagccgccgg
    10921 tgcagcagct tgcgccgctg ccggatccgg tgtcggctca accgcgggcg ccggctggtc
    10981 gggcttcgct ttgccgaaga gcttatttac gatgtcacca aagatgctca ttctgaatcc
    11041 tttgcagatg ggttactggc gcaagcacat tccgcttacg gcttgaacac tggttgggga
    11101 atcgggcgaa aatcgggcga atgaagcaga ggacatgctg gccacgatcg gcaagccgca
    11161 cttgaacgct gatacgccca caaccagact caacgagtgt aggagaaaag cgcggaccga
    11221 tgtgtcaaaa aaggtcgtgc cgcgggcgcg gagttgcaag caaatgcaag cggacttgcg
    11281 agcagacgtg cgcgcagact tgcaaagaga cgtacgagcc ggcggcatgc atgatgcagc
    11341 cgctgcgaga gattggcgga aaacgcataa gttgaaatcc gacagccgga cacccgacga
    11401 cgacgtctga ccgaccaagg cttccccggc gccgccgggt gccgctgcgg accccacttg
    11461 ccgctctcag cccagagcgg cacgatttgc cctttgcgac gtgtatatcg cacgacgcaa
    11521 agagcgcccc acctgcaatt gccatgacga tgacgttaca acgatcatga caacctgtgt
    11581 gactccacta ccggatggtt caggccgctt tggcttcgct gcccggcgct tgcgcgactt
    11641 cgaaattcgc catgatttcc agcgcgcgga ccatcgccga atggtcccat gccttgccgc
    11701 cgttggctgc gcacacgctg aacagttgct gtgcgctcgc cgtatgcggc agggcgatgc
    11761 cgagtttgcg cgcgccgtcc agtgcgaggt tcagatcctt ctggtgcagt tcgatgcgaa
    11821 agcccggatc gaatttgcgc ctggtcatgc gctcgccgtg cacttcgaga atccgcgatg
    11881 aagcgaagcc gcccatcaag gcttttcgca cacgttccgg atcggcaccc gagcgcgaag
    11941 cgaacagcaa cgcttcagca actgcttcga tgttcagcgc gacgatgatc tggttcgcga
    12001 ccttgcaggt ttgacccgcg ccgttgtcgc cgatcagcga gatgttcttg cccatcagtt
    12061 cgaacagcgg cctggccgtg gcgaacgctt tttccggacc gccgaccatg atcgtcagcg
    12121 acgcttcacg cgcgccgact tcgccgcccg agaccggtgc atccaggtag tcgacgccca
    12181 gcgcgttgat cttcttcgcg aaggcttgcg tgtcgagcgg cgagattgag ctcatgtcga
    12241 tcaccagctt gccttgcgcg agaccggcgg cgacgccgtc gtcggcgaac agcacgttgg
    12301 cgacgtcagg cgtgtccggc accatgatga tgacgatgtc ggcagcttgt gcgacagcgg
    12361 tcgaattggc gacgacgctc gcgctcttgc tcagatcttc cggcaccggg tacgcgccat
    12421 tcacgaacag cgtgtggccg cctttaatga ggttgcgcgc catatgcgcg cccatgatgc
    12481 cgaggccgat aaaacc
//
LOCUS       DATASH010000908         5486 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2330808,
            whole genome shotgun sequence.
ACCESSION   DATASH010000908 DATASH010000000
VERSION     DATASH010000908.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5486
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2330808"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VHV99_24280"
     CDS             <1..529
                     /locus_tag="VHV99_24280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX3638537.1"
                     /translation="QRIFGEHVALYNHRFVIKDKFSSSKVFLHQDSCYHLGNHNKCSL
                     FVPLSAVDPDNGGMSFHLGSHRLGSLGDAGEINPESFEIKWPKVTPELQPGDFVIMNS
                     SLWHESGPNVSGVNRIMADIIVQPADDPTGKALLSGSWQTDIFYSPENCIRYFVNSRV
                     LKIIKYEKERAAAQI"
     gene            750..1151
                     /locus_tag="VHV99_24285"
     CDS             750..1151
                     /locus_tag="VHV99_24285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638538.1"
                     /translation="MPAIRVLCKPREATVKAIKHYDTQVTHSLKQRILSVKPGQDIWI
                     WGLSAFMATALTNLGDARHSISGIFDSRYPQDSNLGIKVQVEPSFASKADSPDRALVI
                     CGSTYSAVQKVIRAKVQAAFPDAEFFVVSGS"
     gene            complement(1189..3564)
                     /locus_tag="VHV99_24290"
     CDS             complement(1189..3564)
                     /locus_tag="VHV99_24290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family V/XII"
                     /protein_id="HEX3638539.1"
                     /translation="MKTTMKRSLLVLAGVCTSLTVTLAAPAQAIAAVEVQPALQPLPT
                     VWPRDFDSAQQRIELYQPQVEGWRGNRIEGRAAFAVGAKSATPDYGVAHFKARAEIDK
                     TSSTVRLLDIVIDQVEMPATPDAAAGVKTALQARIPKDGLTTSLEQLQASYARTHEGG
                     EPGIAVQNDVPNILFAERPTLLVLVDGDANWRALGDTYYQRLINTRAMLLKDASGALH
                     LNAAGYWYDAPALGGPWHLNSAPPHELVAAAKSAQTNLKSDPMLPADGKTPAQAPDVV
                     VAAQPAELVVTNGKPSLQPVKGVNLLSVTNADHALFVEPRDNHYYLLLSGRWFSAASL
                     AGPWAFVDGKSLPADFSKIAADDPHANALVSVPGTPQAKEAAIAATIPQTATVSRKTT
                     TLKVVYDGAPKFVGIAGTSLSYAVNTATPVIQTAPSQFFAVANGIWFTASAASGPWHV
                     ADTVPASIYTIPAASPVHYVTYVRVYSTTPDTVVVGYTPGYMGVVVNADGTVVYGTGY
                     VYAPYVGDIYYGCPATYGYGAGFALGTTVGFAFGFAIGATWGAPSPYWGPYWGGGPWN
                     WQYANVNQANIYGRWGGQATVTHASGWNGWTGNSWSGSAASGFNPYTGGHFQASRGAV
                     KNAYNGNFAAGRQGSFSDPSTGRSGAARGGVVGNEATGDYAAGRQRAGYNAQTGRYGA
                     AEADVTGNAYSGDHQAGTKGIVGNKDTGRAVAWNNGNVYAGSDGNVYRHDQGGGWEQH
                     GADGWQPVQPNANQTQRLDSLRQGHDLGNARASGQFQPQRRFGGGGGRFRR"
     gene            complement(3663..>5486)
                     /locus_tag="VHV99_24295"
     CDS             complement(3663..>5486)
                     /locus_tag="VHV99_24295"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HEX3638540.1"
                     /translation="FDDKQDFEDAQRGFIATIPNGVIKTDDGGISWSLNKYKFLEGQA
                     PDTVNPSLWRNAQLNMFNGLFKVTDHVYQVRGLDLANMTIVEGDDGIIIIDVLVTKEA
                     ARAALDLYYQHRPKKPVLAVIYSHSHADHYGGVKGVVDEADVKSGKVKIIAPEGFLAE
                     AVSENVFAGNAMGRRTLYQYGAFLPASPRGQIDAGLGKTTSFGTLGLIAPTDTISKTG
                     ETRTIGGVQFEFQMAPGTEAPSEMLIYMPQFKVLDTAEDTTHTLHNMYTLRGAQVRDA
                     SNWWKVLNEAIVRYGDKTDVVISQHQWPTWGQERAVSYIAGTRDMFKYLHDQSLRLAN
                     EGYTMTEIGEQIQVPDSIGKKWYNRGYYGSVNHDSKAVYQRYLGWYDSNPANLHPLPP
                     EQESKEFVRYMGGADAVMAKAKQDFDKGNYRWVATAMNKVVFADPDNKAARNLEADAL
                     EQLGYQTENPTWRNEYLMGAYELRNGVPKVPGVNTVTPDAVAAMSPALLLDYMGIRLN
                     GPKASGKHMTINWDLGNGEKYAIELRNSVLIYTPNATLPNADATLTMSKDAFASLLMG
                     GRTVDQETQSGNAKVAGDPQKVTELFGMLDSFNPMFNIVTP"
BASE COUNT          970 a         1763 c         1631 g         1122 t
ORIGIN      
        1 tcagcgcatc tttggcgagc atgtggcgtt gtacaaccac cgctttgtca tcaaagacaa
       61 attcagttcg agtaaggtct tcctgcatca ggattcttgc tatcaccttg gaaaccataa
      121 caagtgcagc ctgtttgtcc cgctcagtgc ggtcgatccg gacaacgggg gaatgagctt
      181 tcacctcggt tcacacaggc tggggtctct tggcgatgcg ggggaaatca accccgaaag
      241 cttcgagatc aaatggccga aggtcacgcc tgaattgcaa ccgggcgatt tcgttattat
      301 gaacagctcg ctctggcatg aatccggtcc aaacgtgagc ggcgtcaacc gaatcatggc
      361 cgacatcatc gtccaacctg ccgacgaccc gacgggaaag gccttgctgt ctggctcgtg
      421 gcagacggac atcttctatt caccggagaa ctgcattcgt tatttcgtga attcccgggt
      481 cttgaagatc atcaagtacg agaaagagcg cgcggccgcg cagatctgag cgaagtgctc
      541 atgcctatcg gcgccggatc gagtcgccaa ggcgtacgaa cccatttgaa gatcagaggc
      601 atagccatgc tagtcatgcc tccggcgcgc tcaagttttc cttcaacaat ttggaacact
      661 tgtattactt tacagactgt tcgcttatcg ggttgattca gaaagcaggc cttcgtgtac
      721 tcagcttcga gacgcagtca ctaccccgta tgcccgctat tcgcgtgcta tgtaagccac
      781 gggaagcgac cgtaaaggct atcaaacact acgacacgca ggtcacgcac agcctgaaac
      841 aaagaatatt gtctgtcaaa ccgggacagg atatctggat ctggggtcta tccgctttca
      901 tggcaaccgc actgacaaat ttgggcgacg cgcggcatag catcagcgga atcttcgact
      961 cgcgctaccc tcaggatagc aacctgggca tcaaggttca ggttgaaccc agctttgcct
     1021 ctaaagccga ttcgcccgat cgagcactcg tcatttgcgg ctcgacctat tcagctgttc
     1081 agaaggtgat ccgcgccaaa gtgcaagccg ccttcccgga tgcagagttt ttcgttgttt
     1141 cgggaagttg atatggaggg aaagcgggcg tagggtctgc tttccacttc accgtctgaa
     1201 ccttccaccc ccgccgccaa accgccgctg cggctgaaac tgcccactcg cgcgcgcatt
     1261 cccaagatca tgcccttgcc tgagcgaatc cagccgctgt gtctggttgg cgttcggctg
     1321 caccggctgc cagccatcgg cgccgtgctg ctcccagcca ccgccctgat catgccggta
     1381 aacattgccg tcgctgcccg catacacatt gccgttattc cacgcaacag cgcgccccgt
     1441 gtccttgttg cccacgatgc ctttcgtgcc cgcctggtga tcgcctgaat aagcgttgcc
     1501 ggtcacgtcc gcttcagccg cgccataccg cccggtctgc gcgttgtagc cggcccgctg
     1561 ccggcccgcc gcataatcgc cggtggcctc gttgcccacc acgccaccgc gcgccgcgcc
     1621 gctgcgtccc gtcgacggat cgctgaacga gccttgccgt ccggccgcga agttgccgtt
     1681 gtacgcattt ttaaccgcgc cgcggctcgc ctggaagtga ccaccggtgt acggattgaa
     1741 accggatgcc gccgatcccg accagctatt gccggtccag ccgttccagc cgctcgcgtg
     1801 ggtcacggtg gcctgccctc cccagcggcc gtaaatattg gcctggttga cgttcgcgta
     1861 ttgccagttc cacggaccac cgccccaata cggtccccag tagggcgacg gagcgcccca
     1921 cgtggcgccg atcgcgaagc cgaacgcgaa gcccaccgtg gtgccgagcg cgaagcccgc
     1981 gccgtagcca taggtggccg gacagccgta gtagatgtcg ccgacatacg gcgcatagac
     2041 gtagccggtg ccgtacacga cggtgccgtc cgcattgacg accacgccca tgtagcccgg
     2101 cgtatagccg accaccaccg tatccggtgt cgtcgaatag acgcggacgt acgtcacgta
     2161 atgcacgggc gaagctgccg gaatcgtata gatcgaggcc ggcacggtat cggcgacgtg
     2221 ccatgggccg cttgccgcgc tcgccgtgaa ccagatccca ttcgcaaccg cgaagaattg
     2281 cgaaggcgcg gtctggatca ccggcgtcgc cgtgttcacc gcatacgaca acgacgtgcc
     2341 cgcgatgccg acgaacttcg gcgcgccgtc ataaacgacc ttcaacgtcg tggtcttgcg
     2401 cgacacggtc gccgtctgcg ggatcgtcgc ggcgatcgct gcttctttcg cctgcggtgt
     2461 acccggcacg gagacgagcg cgttcgcatg cgggtcgtca gccgcgatct ttgagaagtc
     2521 ggcgggcagg ctcttgccgt cgacgaaggc ccaggggccg gcgaggctcg ccgcgctgaa
     2581 ccagcggccc gacagcagca ggtagtagtg gttgtcgcgc ggctcgacga agagcgcgtg
     2641 gtcggcgttg gtgacggaaa gcagattcac acccttcacc ggctgcagcg aaggcttgcc
     2701 gttcgttacc accagctcgg ccggctgcgc ggcgaccacc acgtccggcg cctgcgcagg
     2761 cgttttgccg tcggcgggca gcatcgggtc ggacttcaga ttcgtttgcg cgcttttagc
     2821 cgcggcgacc agttcgtgcg gcggcgcgct gttcagatgc cacggaccgc cgagcgctgg
     2881 cgcgtcgtac cagtagcccg cggcgttcag atgcaacgcg ccgctcgcgt ccttgagcag
     2941 catggcgcgc gtgttgatca ggcgttgata gtacgtgtcg ccgagcgcgc gccagtttgc
     3001 gtcgccgtcg acgagaacga gcagcgtcgg gcgctcggcg aagaggatgt tgggcacgtc
     3061 gttctgcacc gcgatgccgg gttcgccgcc ttcgtgagtc ctggcataac ttgcctgcaa
     3121 ctgttcgagc gaggtggtga gtccgtcttt cggaatccgt gcctgcaagg ccgttttgac
     3181 gccggcggcg gcgtccggcg tggccggcat ctccacctgg tcgatgacga tgtcgagcaa
     3241 tcgcaccgtc gaactggtct tgtcgatttc agcgcgcgcc ttgaaatgcg cgacgccgta
     3301 gtccggcgtg gcgctcttcg cgccgacagc gaaggccgcg cgaccttcga tccggttgcc
     3361 tcgccaccct tccacctgcg gctgatacag ctcgatacgc tgctgggcgc tgtcgaaatc
     3421 gcgcggccag acagttggga ggggctgcaa tgccggctgc acctcgacgg cagcgatcgc
     3481 ttgagcaggc gcggcaagcg tcactgtcag cgacgtgcag acgccggcga gcaccagcaa
     3541 cgagcgcttc atcgtggttt tcatggcaca cgatccttca gcgtaaacat cggtgaatcg
     3601 aaaacgtcgg cacgggtggc tcgcgacaag ccggccgccc gtgctccctg cgatgcagac
     3661 cgttacggcg tcacaatgtt gaacatcgga ttgaagctgt ccagcatgcc gaacagttca
     3721 gtcaccttct gcggatcgcc ggccaccttc gcgttacccg actgcgtctc ctggtcgacc
     3781 gtccggccac ccatcaacag cgatgcaaac gcatccttgc tcatggtcag cgtggcatcc
     3841 gcattcggca gcgtcgcgtt cggcgtgtag atcagcaccg aattgcgcaa ctcgatcgcg
     3901 tacttctcgc cgttcccgag atcccagttg atggtcatat gcttgccgct cgccttcgga
     3961 ccgttcaggc gaatgcccat gtaatccagc agcagtgccg ggctcatcgc cgccaccgca
     4021 tccggcgtca ccgtgttcac gcccggcacc ttcggcacgc cgttgcgcag ttcgtacgcg
     4081 cccatcagat actcgttacg ccatgtcggg ttttcggtct ggtagccgag ctgttccagt
     4141 gcgtccgctt cgaggttgcg cgccgccttg ttgtccggat cggcgaacac caccttgttc
     4201 atcgcggtag cgacccagcg gtagttgccc ttgtcgaagt cctgtttcgc ctttgccatc
     4261 accgcatccg cgccgcccat atagcgcacg aattccttcg actcctgttc cggcggcagc
     4321 ggatgcaggt tcgccgggtt cgagtcgtac cagccgagat aacgctggta cacggccttc
     4381 gaatcgtgat tcaccgagcc gtagtagccg cggttgtacc acttcttgcc gatggaatcc
     4441 ggcacctgaa tctgctcgcc gatctccgtc atcgtgtagc cctcgttcgc gagccgcaac
     4501 gactgatcgt ggaggtactt gaacatgtcg cgagtcccgg cgatgtagct gaccgcccgc
     4561 tcctgccccc acgtcggcca ctgatgctgc gagatcacga cgtcggtctt gtcgccgtaa
     4621 cgcacgatcg cttcgttgag caccttccac cagttcgagg catcgcgcac ctgcgcaccg
     4681 cgcagcgtgt acatgttgtg cagcgtatgc gtggtgtctt cggcggtatc gagcaccttg
     4741 aactgcggca tgtagatcag catttcggac ggcgcttcgg tgcccggggc catctggaat
     4801 tcgaactgca cgccgccgat cgtgcgcgtc tcgccggtct tgctgatcgt gtcggtcggc
     4861 gcaatcagac cgagcgtgcc gaacgaggtg gtcttgccga ggccggcgtc gatctgtccg
     4921 cgtgggctcg ccggcaggaa cgcgccgtat tgatagagcg tgcgacggcc catcgcattg
     4981 ccggcgaaca cgttttcgct gacggcttca gcgaggaagc cttccggtgc aatgatcttc
     5041 accttgccgc tcttcacatc ggcttcgtcg accacgccct tcacgccacc gtagtggtcc
     5101 gcgtggctgt gcgaatagat cacggcgagc accggcttct tcggacgatg ctggtagtac
     5161 agatcgagcg cagcgcgcgc ggcttctttg gtgaccagca cgtcgatgat gatgatgccg
     5221 tcgtcgccct cgacgatcgt catgttcgcc agatcgaggc cgcgcacctg atagacgtga
     5281 tcggtgacct tgaagaggcc gttgaacatg ttgagctgcg cattgcgcca caagctcggg
     5341 ttcaccgtgt cgggcgcctg accttccaga aacttgtact tgttcagact ccagctgatg
     5401 ccgccgtcgt cggtcttgat cacgccgttc ggaatggtcg cgatgaaacc gcgttgcgcg
     5461 tcttcgaaat cctgcttgtc gtcgaa
//
LOCUS       DATASH010000909         2376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2330891,
            whole genome shotgun sequence.
ACCESSION   DATASH010000909 DATASH010000000
VERSION     DATASH010000909.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2330891"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..1598)
                     /locus_tag="VHV99_24300"
     CDS             complement(60..1598)
                     /locus_tag="VHV99_24300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX3638541.1"
                     /translation="MTNTTIDVTELTVEQVQSGFASGALTSESLTAAFLERIAQFNPR
                     YNAIIFLNLKALDDARAIDARRAAGEALGPLAGVPVVVKDTMDMAGFPTTAGWTLLYS
                     PTGGVDLMPATDSPVVARMRAAGCVILGKTNVPVLSATGSHANDSWAGPTLNAVDTSF
                     MPGGSSAGTATAVGASMAVLGLAEETGGSIQNPASAQALVGIKPTFALVPNAGVMPLA
                     GSTRDVVGPIARCVRDAALTLDVLAGFSEEDLKTVAGVGMKPRGGYAAGLREDALNGK
                     RLGLYGPGWRDRALSAETIALYERAQKELEARGATLIADPFSGSGFAALGKPAPGSDH
                     FDARGMESLPYDMQKYLERLGPGNTIKTWTDFVDATRAEDPFGPSGVLTYMSSLPDME
                     ANLRDAAAPPVIGAFYALRETYLSIFEEVMTKHALDGLVFPQLRDALPPREGTETLHE
                     TTVCEINIAGLPGVTLPAGYYESGSPFNLIFVGRRWSEPALLAMAYDYETAGRHRRTP
                     VLTT"
     gene            complement(1624..>2376)
                     /locus_tag="VHV99_24305"
     CDS             complement(1624..>2376)
                     /locus_tag="VHV99_24305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3638542.1"
                     /translation="ASHGGADPGNQQDAGTVLFEATQLCSGYGRIPILNQVSFSVKAG
                     ERVGILGHNGMGKTTLLRTLTGHLPTTSGTVRFNGVDITHYSATARAKAGIGYVPQGR
                     EIFPTLSVMENLRVGCLLSKRGEDATIAEILELFPRLKAYLDRPGGALSGGEQQLLAL
                     ARCLCGGPKIVLLDEPTEGIQPSIIDEIIDTLKSVAEQRSLTILLVEQNLNFIASLSQ
                     RILILQKGTVTREIPPEIVTDPAMLDEFAGIA"
BASE COUNT          424 a          729 c          750 g          473 t
ORIGIN      
        1 tgtgctcgga cataaggtgt tctgttcctg ctcttccaag atggcaaccg tcctcatatt
       61 caagtcgtaa ggacgggcgt tcgccgatga cgtccggcgg tttcgtaatc gtatgccatc
      121 gcgagaagcg ccggctcgct ccatcggcgg ccgacaaaaa tcaggttgaa cggagagcct
      181 gattcgtaat atccggccgg caacgtaacg cccggcaagc cagcgatgtt gatctcgcat
      241 accgtcgttt cgtgcagcgt ttccgttcct tcgcgcggcg gcaatgcatc gcgcagttgc
      301 gggaacacga ggccgtcgag cgcgtgcttt gtcatgactt cttcgaagat cgagaggtag
      361 gtctcgcgca gcgcataaaa cgcgccgatc acgggcggcg ccgccgcgtc gcgcaagttt
      421 gcttccatgt ccggcagcga cgacatgtag gtcagcacgc cggacggccc gaacggatct
      481 tcggcgcggg ttgcatcgac gaaatccgtc cacgtcttga tcgtgttgcc cggaccaaga
      541 cgttcgaggt acttctgcat gtcgtaaggg aggctttcca tgccgcgcgc gtcgaagtga
      601 tccgatcccg gcgcgggctt gccgagcgcc gcaaaaccgc tgcccgaaaa ggggtctgcg
      661 ataagagtcg cgccgcgtgc ttcgagctct ttttgcgcgc gttcatagag cgcgatggtt
      721 tccgccgata acgcccggtc gcgccagccc gggccgtaga gcccaagccg cttgccgttg
      781 agggcgtctt cgcgcaagcc cgctgcatat ccgccgcgcg gcttcatgcc gacgcccgcc
      841 accgtcttga ggtcctcttc agagaatcct gcgagcacgt cgagcgtcaa tgcggcatcg
      901 cgtacgcatc gcgcgatggg gccaactaca tcgcgcgtgc tgcctgcaag cggcatcacg
      961 cccgcgttgg gaacaagcgc aaaggtcggc ttgatgccga ccagcgcctg tgccgatgca
     1021 gggttctgga tcgacccgcc ggtttcctcg gcgagaccga gaacggccat gctcgcgccg
     1081 accgccgtcg cagtgcccgc gctgctgccg ccgggcatga acgatgtatc gaccgcgttg
     1141 agcgtgggtc ccgcccagct atcgtttgca tgcgaccccg ttgcgctgag caccggcacg
     1201 ttcgtcttgc cgaggatcac gcaaccggct gcgcgcatgc gagccacgac tggcgaatcc
     1261 gtggcaggca tcaggtccac gccgccagtc gggctgtaca gcagcgtcca gccggcggtt
     1321 gttgggaaac ccgccatgtc catggtgtcc ttcacaacaa ccggtacgcc cgcgagcggc
     1381 ccgagcgctt cgcctgccgc gcggcgagcg tcaatggcgc gcgcgtcatc gagcgctttg
     1441 aggttcagga agatgatcgc gttatagcgc ggattgaact gtgcaatacg ttcgaggaac
     1501 gccgcggtca gcgactcgga agtgagcgcg ccgctggcaa agcctgactg gacttgttcg
     1561 acggtgagtt cggtgacatc gatggtcgtg ttggtcatgg aagaattccg gtttcaaagg
     1621 ggctcaggca atgccggcga actcgtcgag catcgccggg tcggtgacga tctcgggcgg
     1681 gatctcgcgc gtgacggtgc ccttctgcag gatcaggatg cgttgcgaca gcgaagcaat
     1741 gaagttgagg ttctgctcga ccagcaagat ggtgaggctg cgttgctcgg caacgctttt
     1801 caatgtgtcg atgatttcat cgatgatcga aggctggatc ccttccgtgg gttcatcgag
     1861 caatacgatt tttggtccgc cgcagaggca tcgcgcgagc gccagcagtt gttgctcacc
     1921 gcctgagagc gcaccgccgg gacgatcgag ataggctttc aagcgtggaa acagttcgag
     1981 aatttccgcg atggtcgcat cctcgccgcg cttggacagc aggcatccca cgcgcaggtt
     2041 ttccattacg ctcagtgttg gaaatatctc gcgaccctgc ggcacatagc caattccagc
     2101 tttcgcacgc gccgtcgcgc tgtagtgcgt gatgtccacg ccgttaaagc gcacggttcc
     2161 ggaagtggtc ggcagatgcc ctgtcaacgt gcgcagcagc gtggtcttgc ccatgccgtt
     2221 gtgaccgaga atccccacgc gctcgccggc ctttaccgag aaactgacct ggttgagaat
     2281 cgggatgcgc ccgtagccgg agcacaactg cgttgcctcg aaaagcaccg tgcctgcgtc
     2341 ttgttgattt cctggatcag ctccgccgtg cgatgc
//
LOCUS       DATASH010000910         5314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1935225,
            whole genome shotgun sequence.
ACCESSION   DATASH010000910 DATASH010000000
VERSION     DATASH010000910.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5314
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1935225"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..230)
                     /locus_tag="VHV99_24310"
     CDS             complement(69..230)
                     /locus_tag="VHV99_24310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638543.1"
                     /translation="MAPGGASNPLAPITGLLGTVTGALPGTATSAGGSTGGGLLGGPG
                     GPGSLAVKK"
     gene            complement(697..1908)
                     /locus_tag="VHV99_24315"
     CDS             complement(697..1908)
                     /locus_tag="VHV99_24315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3638544.1"
                     /translation="MSDRSSEFATLPAAHRDLSGTARQRARIATMAVFFIAGMMYASW
                     GVHVPTVRDRFHLNAAMLSFALLAVAGGSIGAMAANASWIARVGTRRACLTGGLAMAV
                     CAALILIVPAYWMLLIVLAVFGAGMATLDVAMNAEASAVEKTLGKPIMSSLHGMFSVG
                     GMFGAAVGGALLARGMAPALHLALAAAISALVLIAACPSVLPHVPHADHPDAATPRAN
                     RWRSPALWALGAMALVALIAEGAMYDWATVYMRDVVLSTPALASAAYAAFSAGMAAAR
                     FAGDAVRARFGAPQLVMASASLACAGMVGALLLPHPVAALLGFTLMGLGLANMMPVLF
                     AAAASVKGIHAAEGLAHVAGLAYFGLLLGPVIIGAVTQVTSLPIGLSMVAVCAALIAI
                     AGPKVLRRLKI"
     gene            2187..2849
                     /locus_tag="VHV99_24320"
     CDS             2187..2849
                     /locus_tag="VHV99_24320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX3638545.1"
                     /translation="MNIPAHAPLHLLHSAATGTLATHARQPEGFPYPSVLPFAPDPQH
                     RPTILVSRLAEHTHNLHADSRAGFLVVDAPDGDVLSGQRVTLLGTFEQVDSSPDVVQR
                     YLRYHPDAERYLVLGDFTFWTMRLERLRYIGGFGAMGWLAGAELDPLPSLGVEEESAL
                     IGHFAASSSSTRRLELLGVDRYGADLKVDGARTRIAFDDVKLNTEALREALEDCIQRH
                     AN"
     gene            3246..3548
                     /locus_tag="VHV99_24325"
     CDS             3246..3548
                     /locus_tag="VHV99_24325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HEX3638546.1"
                     /translation="MSSYKELLAQREKLEKQIEEAKSREYAEVLNEIKQKMADYGISL
                     TELGGGRGAKAAKAGRPRAGVAPKYRDPDSGSTWSGRGKPPRWIAGLDREKFLIQK"
     gene            3805..5043
                     /locus_tag="VHV99_24330"
     CDS             3805..5043
                     /locus_tag="VHV99_24330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX3638547.1"
                     /translation="MPSTATVQFAEKHRVARKSTFVSIALNTVLMTLQIVIGVFAHSQ
                     ALIADGIHSLADLISDFVVLIANRHSGAKPDADHNYGHSRYETVASLFLGALLIVVGV
                     GMVWRAGTRLADLHSIPAVHMSALAVAVLVLVCKESLFRYMLREAQRVRSAMLIANAW
                     HARSDAASSLVVALGIIGSLAGVRLLDPIAAAIVGFMVARMGWMFGWDALQDLSDRAL
                     DDVASADMRALLLATPGVRDVHEMRTRKMGDFALIDAHILVDPQISVSEGHYIAESAR
                     LRVLADNRVLDALIHVDPENDAVARPPVNLPTRERIVAEVDAAFAAGGAKPAAVNIHY
                     LSAGLDVEVVLPAAASLAAESDAQCLARVDLAALKRRLGARKLSVLRGLDALSDESSS
                     AMKAGSGIARAKAAEAHSAG"
BASE COUNT         1074 a         1614 c         1597 g         1029 t
ORIGIN      
        1 gacccggctc gccactccct tcggcgcttg tttgccgcgc tgttatttgc tgtcgttatt
       61 tgtcttcgtc actttttcac cgcgagactg cctgggcctc ctggaccgcc cagcaggccg
      121 ccgcctgtcg agccgcctgc cgaggtcgcc gtaccgggca atgcgccggt gacggtgccc
      181 agcaggccgg tgatcggcgc aagcgggttg ctggcaccgc ccggtgccac ccgaacccgc
      241 agccgagccg agcgtgcctg tcagcgacga caccgccgcc gtcaccggtg ccagcggatt
      301 gccgctactg ccggtcaccg tgctgaggac gccggagagt ggtgcaagtg ggttggcctg
      361 gtcaacgtac tgccgcatcc ggtcagtaag atcatcgacg acacgcacat ggcgatcgcc
      421 gacagtttga tattgcgatt catggtccgc tcccccgtaa tctggaaatt gctgaggagc
      481 taactgcaag gcctatgcca tttgcgattg catggccaat cagtcaaata aattattatt
      541 aatcaatgag ttgtgcgtag tcgcccgata tagtcaaaag cttggttccg ccgacgtgcc
      601 gaggaacaca tcggcgtgca gacctcgcgt aacgtaacgt aaggttgcgg ctttcgcatg
      661 gcagggcgcc cgccgataca gcagcgtgcg tctgcgtcag atcttcaaac gccgcagcac
      721 cttcggaccc gcgatcgcga tcagcgccgc gcacacggcc accatggaca agccgatcgg
      781 caaactggtc acctgcgtca ccgcgccgat gatcaccggc ccaagcagca gcccaaagta
      841 cgccagcccg gcgacatgcg cgagcccttc ggccgcgtga atacccttga cgctcgccgc
      901 tgccgcgaac agcacgggca tcatgttcgc gagcccgaga cccatgagcg tgaagccaag
      961 cagtgcggcg accggatgcg gcagcagcag tgcgccgacc atgcccgcgc aggcaagcga
     1021 tgcgcttgcc atcaccagtt gcggtgcgcc gaagcgggca cgcacggcgt cgccggcaaa
     1081 acgcgctgct gccatgccgg ccgaaaatgc cgcgtaggcc gcgctcgcga gggcaggcgt
     1141 ggacagcacg acgtcgcgca tatagacggt ggcccagtcg tacatcgcgc cttcggcgat
     1201 cagcgcgaca agtgccatcg cgccgagcgc ccacaaggca ggtgagcgcc agcggttggc
     1261 gcgtggtgtg gccgcgtcag gatgatcggc atgcggcacg tggggcagca cggaaggaca
     1321 ggccgcgatc agcaccagcg cgctgattgc cgcagccaag gcgagatgca gcgccggtgc
     1381 catgccgcgc gccagcaagg cgccgcccac cgccgcgccg aacatgccgc cgacgctgaa
     1441 catgccgtgc agtgacgaca tgatcggctt gccgagcgtt ttctcgacag cgctggcttc
     1501 cgcgttcatc gcgacgtcga gcgtagccat gcccgcgcca aagacagcca gtacgatcag
     1561 cagcatccag taagccggca cgatcaggat cagcgccgcg cacaccgcca tggccagacc
     1621 gcctgtcaga caggcgcggc gcgtgccgac ccgcgcaatc cacgaagcat tcgccgccat
     1681 agcgccgatc gagccaccgg ccacggccag cagcgcgaac gacagcatcg ccgcattcag
     1741 atggaagcgg tcgcgcacgg tcggcacatg cactccccac gacgcgtaca tcatgccggc
     1801 gatgaagaac accgccatcg tggcgatgcg ggcacgctgc cgtgcggtgc cggagagatc
     1861 gcgatgagca gcagggaggg tggcgaattc ggaagaacga tcggacactg aaaactcgtg
     1921 ggagagagaa cgcgggggaa agcgcgttga aagcggaatt tccgattcta acgaagcacg
     1981 ctgtcttatg cttggcgcga attggtccaa cgcttacgat tgttcggctg cacgcgaaag
     2041 tggcggggcg gctcagcggc agtgcgctgc actgtcgccg gggtgttgct gattcaaccg
     2101 cgggtccgcg gcctctctta ccttaggtgt ttgtattgcc gatttgcagc ccggcatccg
     2161 ccgctcatcc atcgaggaac gcgcacttga acattcccgc tcacgctccg ctgcatctgc
     2221 tgcatagtgc ggctaccggt acgctcgcaa cccacgcgcg tcagccggaa ggttttcctt
     2281 atccttcagt gctgccgttt gcaccggacc cgcagcaccg cccgacgatc ctcgtgagcc
     2341 ggctcgccga gcacacgcac aacctgcatg ccgactcgcg cgccggcttt ctcgtggtcg
     2401 acgcaccgga tggcgacgta ttgagcgggc agcgcgttac cttgctcggc accttcgaac
     2461 aggtcgactc gagccccgac gtcgtgcagc gctatctgcg ctatcacccg gacgcggaac
     2521 gatacctggt actcggcgat ttcaccttct ggaccatgag actcgagcgg ttgcgctaca
     2581 tcggcggctt cggcgcgatg ggctggctcg cgggcgccga actcgatcca ttgccgtcac
     2641 tcggcgtcga agaagaaagc gcactgattg gacatttcgc cgcatcttcc tccagcacca
     2701 gaagacttga attgctcggc gtcgatcgat atggtgccga tctgaaggtg gacggcgcgc
     2761 gcacccgtat tgcctttgat gacgtcaaac tcaataccga agccttgcgt gaagcgctcg
     2821 aggattgcat ccagcgccac gctaattaag tttggatcgc tatttcggga aaacactcat
     2881 gccgcaataa caccgcagag caggtctgca aatatcagcc gaagtttcat cttgaagctt
     2941 tcatgggatg aaatgttggt ggcagctttc tatcttaaat ttcatttttt gatagttaat
     3001 cccataatta ctgatccgcg accacgtttt tcagcaaaat acgaaatagg cgttgaaagg
     3061 gattcggcac aacatcgcac ctgcatgaaa ttttttgaaa cgaattgaga ttaattttac
     3121 gagagcgctt ttgcagtctt tctattttgt gttaatctcg ccatcaaatt ccgaggcatg
     3181 aacaccttca aagggcaagc tggcttaaga cccgcagtca tttatccaaa ccacaaaggg
     3241 aacctatgtc gtcctacaag gaactactcg ctcagcgcga gaagctcgaa aaacagatcg
     3301 aagaagcaaa gtcgcgcgaa tacgcagaag tgttgaatga gatcaagcag aaaatggccg
     3361 attacggcat ttcgctgact gaactcggtg gtggccgtgg cgccaaggct gctaaggccg
     3421 gtcgtccgcg tgcaggcgtc gcgccgaagt atcgtgaccc ggatagtggc agcacgtggt
     3481 cgggccgcgg caagccgccg cgctggatcg caggtctgga tcgcgagaag tttctgatcc
     3541 agaagtaata ccaatagggc aatcaggggc gcggatagcg ctcgccggtg tcagaaaaaa
     3601 ccgcgtaccg ttccagggcg cggtttttta ttgtctatgc aaattgtcgt gacgctttgt
     3661 cgcacgcgct ccatgaaact ggaatgcgaa tgctttttgt tccgataagc agatgattta
     3721 aaaggatttt ttgtggtcta cccggtgtat atcgacatgc gccgggtaga atacaagcat
     3781 tgaactattc acttcgtttc agcaatgcct tccaccgcca ccgtccagtt cgccgagaaa
     3841 catcgcgtcg cgcgcaaaag tacctttgtc agtattgcgc tcaatacggt gctcatgacg
     3901 ctgcaaatcg tcatcggcgt gtttgcccat tcgcaggcgt tgatcgccga cggtatccat
     3961 tcgctagccg atctgatttc cgattttgtc gtgctaatcg ctaatcggca tagcggggcc
     4021 aagcccgatg cggaccataa ttacggacat agccgctatg aaacggtcgc ttcattattt
     4081 ttaggggcgc tgctgattgt ggttggcgtc ggtatggtgt ggcgcgccgg cacgcgtctc
     4141 gccgatctgc acagcatccc cgccgttcat atgagcgcgc tcgcggtcgc agtgctcgtg
     4201 ctggtttgca aagagagcct gtttcgctac atgctgcgcg aagcgcaacg cgtgcgttcg
     4261 gcaatgttga ttgcgaacgc atggcacgca cgctcggacg ccgcttcgtc attggtggtc
     4321 gcgcttggca ttatcggcag cctcgcgggc gtgcgcctgc ttgatccgat cgccgcggca
     4381 atcgtcggtt tcatggtggc gcgcatgggc tggatgttcg gttgggacgc gctgcaagat
     4441 ctatccgacc gcgcgctcga cgatgtcgcc tctgccgata tgcgcgcgct gttgcttgcg
     4501 acgcccggtg tgcgcgacgt gcatgaaatg cgcacgcgca aaatgggtga cttcgctctg
     4561 atcgatgcgc atattctggt cgacccgcag atttcggtgt cggaagggca ctacatcgcg
     4621 gaatcggcgc gcttgcgcgt gctggccgac aatcgcgtgc tcgacgccct gatccacgtc
     4681 gacccggaga acgacgcggt tgcgcggccg cctgtcaatc tgccgacccg ggagcggatc
     4741 gttgccgaag ttgacgcggc gttcgccgct ggtggcgcga agcccgccgc ggtcaatatt
     4801 cactacttga gtgcagggct cgatgttgaa gtggtcttgc cggcggcggc ctcgctcgct
     4861 gccgaaagcg atgcgcaatg ccttgcgcgc gtcgatctgg ctgcgctcaa gcgccgtctc
     4921 ggcgctcgca aactgagtgt gctgcgggga ttggacgcac tgtcggacga atccagctcc
     4981 gccatgaaag cagggagcgg catcgctcgc gcgaaggcag cggaagcgca tagcgccggt
     5041 tgaccgaacg tcctgcgacg cgaaccggcg acatgcgtta gtcgacatgt cgcgaaggct
     5101 cgacatcatc gcagtggggc gtcactgctt ctcagtgtca cagtgttgcg gcgtcaaggc
     5161 accccggcaa ggccgcacag catcaaggca cctcgcttca aaacgccggc atcaaggcat
     5221 caaggcgtca atgcatcaat gcatcaaggc attaatgcat caaggcatca acgcatcaag
     5281 gcatcaacgc accaaggcac caaggcacca aggc
//
LOCUS       DATASH010000911         9650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3120156,
            whole genome shotgun sequence.
ACCESSION   DATASH010000911 DATASH010000000
VERSION     DATASH010000911.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 9650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9650
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3120156"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1153)
                     /locus_tag="VHV99_24335"
     CDS             complement(<1..1153)
                     /locus_tag="VHV99_24335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3638548.1"
                     /translation="MTSAFTTATDPLESAVSKVKRHILPLFLIMFIANYIDRVNIGFV
                     NSHMQTDLGIGAAAYGLGSGLFFVGYALFEVPSNVLMQKYGARAWLTRIMGTWGLVAA
                     AMAFVWNDTSFYVLRFLLGVAEAGFFPGVVFYFTQWLPQKERGKAVAVFLGGSALASV
                     LSGPITGSLLSIRGLGLHGWQWMFLIEGAFSIVLCGVSWVLLKSRIRDASWLTAEERT
                     ILETSIAAEQAEREAHGGAHLPAMKLLKDPQILLFCFLYFAIQLTIYAATFWLPTIIR
                     KMGGLSDFEVGMLNAIPWLIAMFAMYCFAVLSAKWRFQQVWLAVSLVISACGLFASTS
                     GNPVLSFVAICFSAIGFKAAASLFWPIPQGYLDARVAAAVIALINSVGNL"
     gene            1414..2736
                     /locus_tag="VHV99_24340"
     CDS             1414..2736
                     /locus_tag="VHV99_24340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3638549.1"
                     /translation="MKTLATTPANEPQTATAATAEEDALYALVTRRLMPILLTAFCIA
                     YLDRINVGLAKLQMASDLGFSDAVYGFGAGIFYLAYAIFEVPSNLIMHRVGARRWIAR
                     IAVSWGLVSMCMLFVNTPASFYALRLLLGMAECGLFPGIILYLTYWYPAHRRGRIVAM
                     FMAALPISGLVGGPLSGWIMQRFTGVSGLHGWQWLFFLEALPAVVIGVVIWLLLPDRV
                     VDARWLSDEQKKVIAANIAREDAQKPTSSTRSVFTSGRMWLMSLMLFAFATGNMGISF
                     WLPTVVQRAGHGSALNVGLLTAIPYAFAAVAMVTLSMSSDKRRERRWHLAVPAFAGAA
                     LLALSTVWSNDLTMTVVTMTAASMCVFAVAPLLWAQPTALLSGGAAAAGIAMISSLGN
                     LAGLFSPSLFGLLTARTHSTSAGMFLLAGFMVLGGLLALVTPARLVNK"
     gene            2926..3636
                     /locus_tag="VHV99_24345"
     CDS             2926..3636
                     /locus_tag="VHV99_24345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEX3638550.1"
                     /translation="MSSLTEKVVATLSDEIRRGTLRPGDRIPTEVAMMKQLSVSRSVV
                     REAISRLQAAKVVETRHGIGTFVLEPATEKSMQLPAADLSSMLDVMAIIEFRIDVEAA
                     SAALAAARRTEQNLKQIRGALAHFESELERGSTDTLAHDIEFHLQIARASGNRYFFDV
                     LSQLGRAVSPRTRLGSAEIAELDHIELLRNVLSEHQLIYRAIERQDADDARAAMRMHL
                     SNSRERLRRAHELSKAGG"
     gene            3894..4640
                     /locus_tag="VHV99_24350"
     CDS             3894..4640
                     /locus_tag="VHV99_24350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3638551.1"
                     /translation="MKLTGNTILITGGGSGIGRGIAEAFHQLGNQVIIAGRRKANLEE
                     TIAANPGMQSIELDITDPDNITAVSQQLIAAYPSLNVLINNAGIMQIDDLSKPVDDVL
                     LVSTLATNLMGPIRLTGALVEHLQQQDHATIINVSSVLGFVPMAIAGVYSSTKAAIHS
                     YTQSLRYKLKDTSVRVIEIAPPWVQTDLLNSKEEPRAMPLAPFIEQTMHELGTDADEA
                     LVEIARPLRDNAGPNEAAFVTQFNDQFAGV"
     gene            4787..5371
                     /locus_tag="VHV99_24355"
     CDS             4787..5371
                     /locus_tag="VHV99_24355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEX3638552.1"
                     /translation="MPKPSHKDTLITQGMRVIHQYGFNGASVRDIVKAAGVPHGSFTN
                     HFASKEAFGLEVIELYFEHARCELRDTLCNDELKPLFRLGKYFDVHIRLISQNDSRNG
                     CLLGNFAADSSDSEPIRARINAIFAEIEKCVLYCLTAAVVARELRADTDCEKLAAFIV
                     SSMQGAFLLAKAQRDPAPVVNLKDVLFRWVLQRA"
     gene            6491..7297
                     /locus_tag="VHV99_24360"
     CDS             6491..7297
                     /locus_tag="VHV99_24360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3638553.1"
                     /translation="MEFRDDTLTHFEAHGAAPLPISTEHSHIEHEGAQIWYASYGTGA
                     PVILLHGGLGHSGNWGYQIPALTGSGHRVVVIDSRGHGRSTRDARPYRYELMASDVLT
                     VMDTLKLHRAAIVGWSDGACVALVLASQAPERVAGVFFFGCNMDPSGTREFAPTPIIE
                     RCFSRHTQDYAHLSATPGEFDAFVGAVSEMMKTQPDYSAHDLSQIRVPVAIVQSEHDE
                     FIKPEHADYLARSIPGAELILLRGVSHFAPLQRPEQFNRVMLDFLDRLLS"
     gene            7695..7922
                     /locus_tag="VHV99_24365"
     CDS             7695..7922
                     /locus_tag="VHV99_24365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638554.1"
                     /translation="MKGFRFGSTQGAFYIMPGRDGWEATYGNETLGEFSSPQQAADEV
                     ARGLTCPHLSEVDTSTLEIPEKLSDWEIVHV"
     gene            complement(8095..>9650)
                     /locus_tag="VHV99_24370"
     CDS             complement(8095..>9650)
                     /locus_tag="VHV99_24370"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEX3638555.1"
                     /translation="SVLNKNALRSFIEAEIADAKAKGVLFSVHLKATMMKVSDPIIFG
                     HVVSVFYKDVLTKHADALAQAGFNPNNGIGDLYARLKDLPTETREAIEADIKAQYAQR
                     PQLAMVNSDKGITSLHVPSDVIVDASMPAMIRESGKMWGADGALHDAKAVIPDRCYAG
                     VYQAVIEDCKKNGAFDPVTMGTVPNVGLMAQAAEEYGSHDKTFQISANGVVRVTDAAG
                     TVLIEQAVEAGDIWRMCQTKDAPVQDWVKLAVNRARATNTPAIFWLDAARAHDAQIIR
                     KVEHYLKDHDTSGLDIRVMTPVEATKFSIERIRAGKDTISVTGNVLRDYLTDLFPIME
                     LGTSAKMLSVVPLMAGGGMFETGAGGSAPKHVQQLVEEGFLRWDSLGEFLALAASLEH
                     LSGAYHNPKAQVLARTLDQATGKFLDNDKSPARKIGGIDNRGSHFYLAMYWAEALAAQ
                     TEDAALQAQFAGVAKAMADNEAKIIEELAAAQGKPVDIGGYYRPNVELTSKAMRPSAT
                     LNKIVDAVA"
BASE COUNT         1962 a         3045 c         2794 g         1849 t
ORIGIN      
        1 cgaggttgcc taccgaattg atcagcgcta tcactgcggc agccacgcgc gcatccagat
       61 agccctgcgg aatcggccag aagagcgaag ccgccgcctt gaagccaatc gccgagaagc
      121 agatagcgac gaacgacagc accggattgc ctgacgtcga ggcgaacagc ccacacgccg
      181 agatcaccag cgacacggcc agccacacct gctggaaacg ccatttcgcc gacagcaccg
      241 caaagcagta catcgcgaac atggcgatga gccaggggat cgcattgagc atgccgactt
      301 cgaagtccga gagtccaccc atcttgcgga tgatcgtcgg cagccagaag gtggccgcgt
      361 aaatggtcag ctgaatcgca aagtacagga aacagaataa aagaatctgc gggtccttca
      421 gcagcttcat ggctggcaga tgcgcgccgc cgtgcgcttc gcgctccgcc tgctccgccg
      481 cgatcgacgt ttcgaggata gtccgctctt cagccgtcaa ccacgaggca tcgcgaatgc
      541 gcgacttcag cagcacccag ctgacaccgc acagcacgat cgaaaaggcg ccttcgatca
      601 ggaacatcca ctgccagccg tgcagaccca agccgcgaat ggacagcagg ctgcccgtga
      661 tcggcccgga cagaaccgac gcgagcgccg agccccccag aaagaccgcc acggccttgc
      721 cgcgttcttt ctgcggcagc cattgggtga agtaaaacac cacgccgggg aagaagccgg
      781 cctcggcgac accgagcaga aagcgcaata catagaacga ggtgtcgttc cagacaaacg
      841 ccatggcggc cgcgaccagc ccccacgtgc ccatgatgcg ggtcagccac gcgcgcgcgc
      901 cgtacttctg catcaacaca ttcgacggca cttcgaacag cgcgtagccg acgaagaaca
      961 agccgctccc caaaccatat gcggccgcgc cgataccgag gtccgtctgc atatgggaat
     1021 tgacgaaacc gatgttcacg cggtcgatgt agttcgcgat gaacatgatc agaaatagcg
     1081 gcagtatgtg ccgcttgacc ttcgagacgg cggattcgag cgggtcggtc gccgtggtga
     1141 aagcagatgt caaggttgtc tccagtttcg gccacccggg cggccggatc gaatgcaacg
     1201 ctgcgcattc gaacaggcgg gtgatcccga aacgcgtgat atgttgtctg atgacagtct
     1261 acatcgatcg agtcattcac tccaatgcgg tgtttaccct gttgtcaatg cgctatacga
     1321 cgcgcgcgaa gcgctaatat actgcgcttg acgccggaca gccaacttgc actaagacgt
     1381 ccgatgacta aaacgacacc ttggagatga cacatgaaga ccctggcgac gacacccgcc
     1441 aatgagccgc aaacggcgac ggccgccacg gccgaggaag acgccctcta tgcgctggtc
     1501 actcgcaggc tcatgccgat cctgttgacc gcgttctgca tcgcgtatct cgaccgcatc
     1561 aacgttggcc tcgccaagtt gcagatggct tccgacctgg gcttcagcga cgccgtctat
     1621 ggtttcggcg cgggcatttt ctacctcgcc tacgccattt tcgaagtccc cagcaatctc
     1681 atcatgcatc gggtcggcgc gcgccgctgg atcgcgcgca ttgccgtgtc gtggggcctc
     1741 gtgtcgatgt gcatgctgtt cgtgaacacg ccggcctcgt tctacgcgct gcgactgctg
     1801 ctcgggatgg ccgaatgcgg tctcttccca ggcatcatcc tgtatctgac ctactggtac
     1861 ccggcgcacc gccgaggccg gatcgtcgcg atgttcatgg cggcgctgcc catttccggg
     1921 ctggtcggcg gtccgctgtc gggctggatc atgcagcgtt tcaccggggt gtcgggcctg
     1981 cacggctggc aatggctgtt tttcctcgag gcgctgccgg cggtggtgat cggcgtcgtc
     2041 atctggctcc tgttgccgga ccgggtggtc gacgcgcgct ggctctccga tgagcagaag
     2101 aaggtgatcg ccgccaatat cgcgcgagag gatgcgcaga agcccacctc cagcacgcgc
     2161 agcgtattca cgagcggccg catgtggctg atgagcctga tgctgttcgc gttcgccacc
     2221 ggcaacatgg gcatcagctt ctggctgccg accgtcgtgc agcgcgcggg ccacggcagc
     2281 gcgctgaacg tcggcctcct gaccgccatt ccgtacgcgt tcgcggcggt cgccatggtg
     2341 acgctgtcga tgagttcgga caagcggcgc gaacgccgct ggcacctcgc cgtgcccgca
     2401 tttgccggcg ccgcgctgct ggctctgagc acggtgtgga gcaacgacct cacgatgacg
     2461 gtcgtgacga tgaccgcggc atcgatgtgc gtgttcgccg tggcgccgct cctgtgggct
     2521 cagccgaccg cactgctgag cggcggagcg gctgccgccg gcatcgcgat gatcagttcg
     2581 ctcggcaatc tggcgggact cttctcgccg tcgctgttcg gcctgctcac ggcgcgcacg
     2641 cacagcacct cggccgggat gtttctgctg gcgggtttca tggtgctggg tggacttctc
     2701 gcgctggtga cgcccgcgcg cctggtcaac aagtgatttc acacgagcat gccgggagcg
     2761 cgagattcga ttccggggga caggtcgtgt cgccggatcg aatcttgtcc ttcgggtcgc
     2821 tcacgagttt gactggcgct atgatgcccg tgtcgagact tcaattgacg tccctcgcgc
     2881 gcgcaacgcg cgtatttcca gccgattagt caaccagcca cagaaatgag cagcttaact
     2941 gagaaggtgg tggccaccct ttccgatgaa atccgccgcg gcaccctgcg gccgggcgac
     3001 cgcatcccca ccgaggtcgc aatgatgaag cagctctcgg tgagccgctc cgtggttcgc
     3061 gaagccatct cacgtttgca ggcggcgaaa gtggtcgaga cgcgtcatgg catcggcacg
     3121 ttcgttctcg aaccggccac ggaaaagtcc atgcaactgc ccgccgccga cctgtccagc
     3181 atgctcgacg tgatggcgat catcgaattc cgtatcgacg tcgaggcagc ctcagcggcg
     3241 ctcgccgccg cgcggcgcac cgagcagaat ctcaagcaga ttcggggcgc gctggcgcat
     3301 ttcgaatccg aactcgaacg ggggagcact gacacattgg cgcacgacat cgagtttcat
     3361 ctgcagatcg cgcgcgcgag tggcaaccgc tacttcttcg atgtcctcag ccagctcggc
     3421 cgcgcagtga gcccgcgtac acggctcggc tccgcggaga tcgcggagct cgaccatatc
     3481 gaactcctgc gcaacgtgct cagcgagcat cagttgattt atcgggccat cgaacgtcag
     3541 gacgccgacg acgcgcgcgc cgcaatgcgg atgcatttga gtaacagccg cgagagactg
     3601 cgacgcgcgc acgaattgag caaagcgggc ggctgacaag cggcgtttgc ttcatctggc
     3661 tcgccgttcc aattcgctca gccgagcctt acaagtacat ttgtattgtt agacaaacct
     3721 gggcatcaag ttgccttatc gaccaaacgc cccgtaggaa tctattgttt gcctaagcga
     3781 acaactgcat tcatccaacg ctgcttgatc aagttaatac gctcgtccat atttcagcat
     3841 cgcttgttgc tgaatttctt cggcacccgt ttaaccgact cgagaggcat ctcatgaaac
     3901 tcactggaaa caccatcctt atcaccggcg gcggatcggg catcggccgc ggcatcgccg
     3961 aagcgttcca ccaactcggc aaccaggtca tcatcgccgg acgccgcaag gcgaatctcg
     4021 aagagacgat cgccgccaat cccggcatgc agtcgatcga actcgatatc acggatcctg
     4081 acaacattac cgccgtgtcg caacagctga tcgcggcgta tccctcgctg aacgtgctga
     4141 tcaacaacgc cggcatcatg cagatcgacg acctcagcaa gcctgtcgac gacgttttgc
     4201 tcgtctcgac gctcgccacc aacctgatgg gcccaatccg cctgacgggc gcactcgtcg
     4261 agcatctgca gcaacaggac cacgcgacga tcatcaacgt ctcgtcggtg ctcggcttcg
     4321 tgccgatggc aatcgccggc gtgtattcgt cgacgaaagc cgccatccac tcgtacacgc
     4381 agtcgctgcg ctacaagctg aaagacacct cggttcgcgt gatcgaaatc gcgccgccat
     4441 gggtgcagac cgacctgctg aacagcaagg aagaaccgcg cgcgatgccg ctcgcgccct
     4501 tcatcgagca gaccatgcat gaactcggca cggatgccga cgaagcgcta gtcgagatcg
     4561 cccgcccgct gcgtgacaac gcgggcccga acgaagccgc tttcgtcacg cagttcaacg
     4621 accagtttgc tggcgtataa ccgtcatagg ggcgagccgg tatcgcactg tggcgatgcg
     4681 gccggctccg catagactcc tcaggccgcc taaagccgct cacgccacgg cccgcattga
     4741 accatgacac aatgcgggcc attcgtatcg ccgaaccatt tcgaccatgc cgaagccttc
     4801 gcataaagac acgctgatca cccagggaat gcgcgtgatc caccaatacg gattcaacgg
     4861 cgcgagcgtg cgcgacatcg tcaaggcggc gggtgtgccg cacgggtcgt ttacgaacca
     4921 cttcgcgtcg aaagaggcgt tcgggctgga agtcatcgaa ctttatttcg agcacgctcg
     4981 ctgtgagctt cgcgatacgc tctgtaacga cgaactcaaa ccgctgttcc gtctcggcaa
     5041 atacttcgac gtccatatcc ggctcatcag ccagaacgat tcgcgcaacg gctgcctgct
     5101 cggcaacttc gctgcggatt cgagcgacag cgaaccgatc cgcgcgcgga tcaatgcgat
     5161 ttttgccgaa atcgaaaagt gcgttctcta ttgcctcacg gcggcggtgg ttgcgcgtga
     5221 gttgcgcgcc gatacggact gcgagaaact cgctgcgttc attgtgtcgt cgatgcaagg
     5281 cgcgtttctg ctcgcgaaag cacagcgcga tcccgcgccg gtcgtcaatc tgaaggacgt
     5341 gctgttcaga tgggttttgc aacgggcata agaaggagca tctcatggcg ccgcccaacc
     5401 acgcagcaga ttgtggcgca cggttttggc gcctttcctt gatctggctc tttggccttt
     5461 ccttgatctg ggtctttggc ctttccttga tctgggtctt tggcctttcc ttgaattgtt
     5521 agtggtctat tagcgtcgcc cctgtgcggg gcgggcacct acttttcttt gcctgccgca
     5581 aagaaaagta ggcaaaagaa agcggctcac accactagtt cttaagcggg ccccccgcac
     5641 agtcgcggta gtggtgcatc tggaatccgt gccctcgcac aatcggcgtt agtgaccagg
     5701 ccatcatcag ctcccactcc gcactgcgtg cgtcgcggat gggtcggcat gggaaaccac
     5761 gggtttcgtt tgcgcgtggt gggggtctcg gcgaggcgcc cacgcgacag tggcaagcga
     5821 gaagatcgat agaagcgaag aagcgaagaa gcgaaaaggc gatatacgcc aacacactgt
     5881 gctgaatgaa gcgtgactgc gaaaacgtag tgctccgttt tatgtaatgc gcgtcggcgc
     5941 gcagtgccgc cggaagcatg ctgaccttat agagaagcca atggctcaca cgagtcccaa
     6001 gcgcagccgc tctctcgata tcccgttccg ccgagcacgc agtgcgaggc gggaagcatg
     6061 actgccttgt caccaacgcg gagtgtgcga gggcacagat tccagatgca ccactaccgc
     6121 gactgtgcgg gggacccgct tatgagctgg cggggtgagc cgctttcttt tgcctacttt
     6181 tctttgcggc cggcaaagaa aagtaggtgc cgccccgcac aggggcaacg ctaatagacc
     6241 aataacaatt caaggaaagg ccaaagagcc agatcaagga aaagccaacg ccacaggcac
     6301 acagacaaac aagcgccgca caggcaaaaa aaaccagcaa aagccctaca ccaaaagaca
     6361 gccagatccg aacaaccacc gccagacaaa aaaaagtcca aaaacccgta gccaacttga
     6421 tttcccacca gcgccgctgg caacctacca agatcctcgc actgcgagcc cacccgaaag
     6481 ggaccctctt atggaattcc gcgacgacac cctcacccat ttcgaagccc atggcgcagc
     6541 cccactacca atctcgaccg agcacagcca catcgagcac gaaggcgcac aaatctggta
     6601 cgcgtcatac ggcaccggcg cgccggtgat cctgctgcat ggcggtctgg gccacagcgg
     6661 caactggggc tatcaaatcc cggcgctgac cggctccgga catcgcgtgg tggtaatcga
     6721 cagccgcggc cacggccgca gcacccgcga tgcgcgcccc tataggtacg agttgatggc
     6781 ctccgatgtc ctaaccgtca tggacacgct gaagctccat cgcgccgcga tagtaggctg
     6841 gagcgacgga gcctgcgtcg cgctggtact cgccagccag gcccccgagc gcgtggccgg
     6901 cgtgttcttc ttcggctgca acatggaccc gagcggcacc agggaattcg cgccgacgcc
     6961 aatcatcgag cgctgcttca gccggcatac ccaagactac gcacacctat cggcgacgcc
     7021 cggcgagttc gatgccttcg tcggcgcggt cagcgaaatg atgaaaacgc agcctgatta
     7081 ctcggcgcac gatctcagcc agattcgcgt gccggttgcg atcgtgcaaa gcgagcacga
     7141 cgagttcatc aagccggaac acgccgacta tctggcccgc agtattcccg gcgcggagct
     7201 gattcttctg cgtggcgtga gccacttcgc gccgctacaa aggccggagc aattcaaccg
     7261 cgtgatgctc gattttctgg accggcttct gtcctgatca cgacgaccgc acatgcaggc
     7321 tcgcttcgcc aagcgggcat cacgccccat tatttggtaa aagccccgaa atttcacgat
     7381 gtgacatgag ccattgcttc gtaaaaaatc gtggtgcgtg gcacaaattc gtcatttcgc
     7441 aatggtgccc cacgttgcac atcgcaaaat gaaacgtcta acacgttatt ttaaaacgat
     7501 attttttgat cgaacgaacc atctatcaag ccctttcacc atgacctgct gacgtagact
     7561 cttttacaag agccgacgct tcatgtggtg cagcgaaata gacgggacaa aacgcagttg
     7621 cagtatgtgc ccccgctcca tgcatgaagc cgctgcggca cagcggtttt taaatggtaa
     7681 ttgggagact aaaaatgaaa ggctttcgct ttggttcgac tcagggagcg ttctacatca
     7741 tgccgggtcg ggatggctgg gaggcaacct acggcaacga aacgctggga gagttttcga
     7801 gcccgcaaca agctgccgat gaagttgcgc gcggcctgac ctgcccgcac ctctccgaag
     7861 tcgacacgtc gacgctcgaa attccagaaa aactcagcga ttgggaaatc gttcacgtgt
     7921 gatggcggca ggaccgccac ctacgtgcga gtagaaacga cgatggcgct ccataggagc
     7981 gccatcgtct tgcttactgc ttacttcagt gcttgctttg gtaccttgag cggcagcctc
     8041 agggcctcat tccgcacctt attcagcacc ttattcagca ccgcatccgg caaatcacgc
     8101 gaccgcgtcc acgatcttgt tcagcgtggc gctcgggcgc atggccttgc tcgtcagttc
     8161 gacgttcgga cggtagtaac cgccgatatc caccggcttg ccttgcgctg ccgccaactc
     8221 ttcgatgatc ttcgcttcgt tgtccgccat ggccttcgcc acgccggcga actgcgcctg
     8281 caacgcggca tcttcggttt gcgcggcaag tgcttcggcc cagtacatgg ccaggtagaa
     8341 gtggctgccg cgattgtcga taccgccaat cttgcgcgcc ggcgacttgt cgttgtcgag
     8401 gaacttgccg gttgcctgat ccagcgtcct cgccagaacc tgcgccttcg ggttgtgata
     8461 cgcgccgctc aggtgttcga gggaagccgc caacgccagg aattcaccga gcgaatccca
     8521 acgcaggaaa ccttcttcga ccagttgctg aacgtgcttc ggtgccgaac cgccggcgcc
     8581 ggtttcgaac atgccgccgc cggccatcag cggaacgacc gacagcatct tcgcgctggt
     8641 gcccagttcc ataatcggga acaggtcggt caggtagtcg cgcagcacgt tgccggtgac
     8701 cgagatcgtg tccttgccgg cgcggatgcg ctcgatcgag aacttcgtcg cttcgaccgg
     8761 cgtcatgacg cgaatatcca gaccgctggt gtcgtggtcc ttcaggtagt gctcgacctt
     8821 cctgatgatc tgggcgtcgt gcgcgcgagc cgcgtccagc cagaaaatag ccggcgtgtt
     8881 ggttgcgcgt gcgcggttca ccgcgagctt gacccaatcc tgaaccggcg cgtccttggt
     8941 ctggcacatg cgccagatgt cgcccgcttc aactgcctgc tcgatcagca ccgtaccggc
     9001 tgcgtcggtc acgcggacca cgccgttcgc cgaaatctgg aacgttttgt cgtgtgaacc
     9061 gtattcctca gcggcttgcg ccatcaggcc gacgttcggc accgtgccca tggtgaccgg
     9121 atcgaaagcg ccgttcttct tgcagtcttc gatcaccgcc tggtagacgc cggcgtagca
     9181 acggtccgga atcactgcct tggcgtcgtg cagtgcaccg tccgcgcccc acatcttgcc
     9241 cgactcgcga atcatggccg gcatggaagc gtcgacgatc acgtcgctcg gcacgtgcag
     9301 gctggtgatg cccttgtccg agttgaccat ggccagttgc ggacgttgcg cgtattgagc
     9361 cttgatgtcg gcttcaatcg cttcgcgcgt ttcagtcggc aaatccttca ggcgcgcgta
     9421 caggtcgccg atcccgttgt tcgggttgaa gccggcttgc gccagcgcgt cggcgtgctt
     9481 ggtcagcacg tctttgtaga acaccgacac cacgtgaccg aagatgatcg gatccgacac
     9541 cttcatcatg gtggctttca ggtgcaccga gaacagcacg cccttcgcct tcgcgtcggc
     9601 gatttccgct tcgatgaagc tgcgcagcgc gttcttgttc agcaccgaag
//
LOCUS       DATASH010000912         4207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2331195,
            whole genome shotgun sequence.
ACCESSION   DATASH010000912 DATASH010000000
VERSION     DATASH010000912.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2331195"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VHV99_24375"
     CDS             complement(<1..340)
                     /locus_tag="VHV99_24375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638556.1"
                     /translation="MRVRGRSFSLLLMAGLLRLFFCPAGGWKMATITENVFIGCWGLF
                     GFFDVVFKWGNVLFFAFHAGHSVFGVFWHCVEECDLGPAGASVWWGFGFIFIRVSFLV
                     FLLVWFIWFFY"
     gene            319..2049
                     /gene="aepX"
                     /locus_tag="VHV99_24380"
     CDS             319..2049
                     /gene="aepX"
                     /locus_tag="VHV99_24380"
                     /EC_number="5.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050188 - phosphoenolpyruvate mutase
                     activity [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate mutase"
                     /protein_id="HEX3638557.1"
                     /translation="MNAREPAFISASRSARLRQMLVSNKLEFMMEGHNGLSARIVREA
                     GFKAIWASGLAISAQFGVRDNNEASWTQVVDNLEFMADASDLPILLDGDTGYGNFNNV
                     RRLVRKLEQRGIAGVCIEDKQFPKTNSFINGEAQPLADMDEFCGKIKAGKDSQSDENF
                     SIVARVEALIAGWGMDEALRRAEAYRQAGADAILIHSKLSRPDEILEFAREWAGRGPL
                     VIVPTKYYSTPTEVFREAGISTVIWANHLIRAATSAMQAVAKDIHSSETLVNVEDSIA
                     AVNEIFRLQDADEYSDAEDRYLSSGRVAGAAIVLAASRGKGLEAVTEDRPKVMLPIAG
                     KPLLRWLIDAFKKQSVNDITVVGGYRADAIDTAGIKLVVNERHAQTGELASLACAVDK
                     LAGDTVISYGDLLFRSYILRDLVESEAAFSVVVDSSLTDAENASVRDFAWCSAADDRG
                     LFGNKVTLRHVSSGQEATSAIASQTPHGRWVGLLNVRGEGRERLQAAIAKLQARADFD
                     SLDMPALLNALIEAGEAIEVQYVHGHWRGVNDLEDFRRAGDFAHAQAPFAAAVGSGAG
                     NADAVSGATQ"
     gene            2046..3212
                     /gene="aepY"
                     /locus_tag="VHV99_24385"
     CDS             2046..3212
                     /gene="aepY"
                     /locus_tag="VHV99_24385"
                     /EC_number="4.1.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033980 - phosphonopyruvate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonopyruvate decarboxylase"
                     /protein_id="HEX3638558.1"
                     /translation="MIEAAQFVEVARERGFDWYAGVPCSYLTPFINYVLQDESLHYVS
                     AANEGDAVALIAGVTLGAKNGRRGITMMQNSGLGNAVSPLTSLTWTFRLPQLLIVTWR
                     GQPGVADEPQHALMGPVTPAMLETMEIPWETFPTEADAIGPALDRALAHMDETGRPYA
                     LVMQKGSVAPYELKDSGTLTRRAPAAPRSQFHDVAASELATRHDALQKVIAHTPLEST
                     VVLASTGFCGRELYAIDDRPNQLYMVGSMGCVTPFALGLALARPDLHVVALDGDGAAL
                     MRMGAFATLGAYGPSNLTHVLLDNGAHDSTGGQATVSPQVSFAGVAAACGYASAVESD
                     DVNVIDALFEAAPSPHGPRFARLTIRRGTPDGLPRPTITPPDVKTRLMRHIGAA"
     gene            3224..>4207
                     /locus_tag="VHV99_24390"
     CDS             3224..>4207
                     /locus_tag="VHV99_24390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate aminotransferase"
                     /protein_id="HEX3638559.1"
                     /translation="MLLLNPGPVTLTERVRNSLLQTDLCHRESEFFDLQDEARTRLVD
                     LYGLDANEWQAVLMTGSGTAAVESMTAALVPQNGKLLVVENGVYGERISQIATQYGIA
                     HESLKHDWMQAPDLAKLAERLDADKAFTHVAVIHHETTTGRLNDLQALGALCRERGLR
                     LLVDGVSSFGAEAIDFADASLDAVAATANKCLHGVPGASFVLVRRAALAQAASRTYYL
                     DLGRLARLQDQRNTPFTPSVHAYYALVEALRELADEGGWQARHARYAALAEQARAGLA
                     ERGIGSVLAPEQSSVVLRAYRLPEGVAYPRLHDALKARGFVIYAGQGGLSAE"
BASE COUNT          854 a         1324 c         1305 g          724 t
ORIGIN      
        1 tataaaaaaa ccaaataaac caaaccaata aaaaaaccaa gaaagacact cgaataaaga
       61 taaaaccaaa accccaccaa accgaagccc ccgcagggcc caaatcacat tcctccacac
      121 aatgccaaaa aacaccaaaa acagaatgcc ctgcatgaaa tgcaaaaaac aacacatttc
      181 cccacttaaa aacaacatca aaaaacccaa acaaacccca acaaccaata aaaacatttt
      241 ccgttatagt cgccattttc cagccgcccg ccgggcaaaa aaagagcctc aataagcccg
      301 ccatcaggag taaagaaaat gaacgcccgc gaacccgcat tcatctccgc ttcgcgcagt
      361 gcacgcttgc gccagatgct cgtcagtaac aaactcgaat tcatgatgga gggccacaac
      421 ggcctctccg cccgtatcgt ccgcgaagcc ggcttcaagg cgatctgggc ttcgggcctc
      481 gcgatctccg cccaattcgg cgtgcgcgac aacaacgaag ccagctggac gcaagtcgtc
      541 gataacctcg aattcatggc cgacgccagc gatctgccca tcctcctcga tggcgatacc
      601 ggctacggta acttcaacaa cgtgcgccgc ctcgtacgca aactcgaaca acgcggcatc
      661 gccggcgtct gtatcgaaga caaacaattc ccgaaaacga atagcttcat caacggcgaa
      721 gcccagccgc tcgccgatat ggacgagttc tgcggcaaga tcaaagccgg taaagactcg
      781 cagtcggatg aaaatttctc gatcgtcgcc cgcgtcgaag cactcatcgc cggctggggc
      841 atggacgaag cgctgcgtcg cgcggaagcc tatcggcaag ccggcgccga cgcgatactg
      901 attcacagca agctctcgcg ccccgatgaa atcctcgagt tcgcgcgcga atgggccggc
      961 cgcgggccgc tcgtcatcgt gccgaccaag tactacagca cgcccaccga agtgttccgc
     1021 gaggccggca tcagcacggt catctgggcg aatcatctga tccgcgcggc gacctcggcc
     1081 atgcaggccg tcgccaagga cattcactcg agcgaaacgc tcgtcaacgt cgaagacagc
     1141 attgccgcgg tcaacgaaat cttccgtctg caagacgccg acgaatactc ggatgccgaa
     1201 gaccgctatc tgtcgagcgg acgcgtggcc ggtgcggcca tcgtgctcgc ggcaagccgc
     1261 ggcaaggggc ttgaggccgt cacggaagac cgtccgaaag tcatgctgcc gatcgccggc
     1321 aagccgctgt tgcgctggct catcgatgcg ttcaagaagc agagcgtcaa tgacatcacc
     1381 gtggtcggcg gttatcgcgc cgatgcgatc gatacggccg gtatcaaact cgtcgtcaat
     1441 gaacgtcacg cgcaaaccgg cgaactggcg tcgctggcct gcgcggtcga caagcttgcg
     1501 ggcgacaccg ttatttcgta cggcgatctg ctgttccgca gctatatcct gcgcgacctg
     1561 gtcgagagcg aagcagcgtt cagcgtggta gtcgattcgt cgctgaccga tgcggaaaac
     1621 gccagcgtgc gcgacttcgc atggtgttcg gcagccgacg atcgcggtct gttcggcaat
     1681 aaggtcacgc tgcgtcacgt gtcgagcggg caggaggcaa cttccgcgat cgcttcgcaa
     1741 acgccgcatg gccgctgggt gggtctgctg aacgtacgtg gcgaagggcg tgagcgtctg
     1801 caagcggcca tcgcgaaact gcaggcgcgc gcggatttcg attcgctcga tatgcccgcg
     1861 ctgctgaatg cgctgatcga agcgggcgaa gcgatcgagg tgcaatacgt gcatggtcat
     1921 tggcgcgggg tgaacgatct ggaagacttc cgtcgtgccg gcgatttcgc gcatgcgcag
     1981 gcgccgttcg cggcagcggt cggtagcggc gcaggcaacg cggacgcagt tagcggagcc
     2041 acgcaatgat cgaggccgcg cagtttgtcg aggtggcgcg cgaacgcggc ttcgactggt
     2101 acgcaggcgt gccgtgctcg tatctgacgc cgttcatcaa ctacgtattg caggacgagt
     2161 cgctgcatta cgtgtcggcc gccaatgaag gcgatgcggt tgcgttgatc gcgggcgtca
     2221 cactcggcgc gaagaacggc cggcgcggca tcacgatgat gcagaactcc ggcctcggca
     2281 atgccgtgag tccgttgacc tcgcttacgt ggaccttccg tttgccgcaa ttgctgatcg
     2341 tcacgtggcg tgggcagccc ggcgtcgcgg acgaaccgca gcacgcgctg atgggcccgg
     2401 tcacgcccgc catgctcgag acgatggaaa tcccgtggga gactttcccg accgaagccg
     2461 acgcgatcgg cccggcgctg gatcgcgcgc tggctcacat ggatgaaacc ggccgtccgt
     2521 acgcgctggt aatgcaaaag ggcagcgtgg cgccgtatga gttgaaagac agcggcacgc
     2581 tcacgcgccg tgcgccggcc gcgccgcgtt cgcaattcca cgacgtcgcg gcgagtgaac
     2641 tggcaacgcg tcacgacgca ttgcagaaag tcatcgcgca cacgccgctc gaatcgacgg
     2701 tggtgctcgc gtcgactgga ttttgcggcc gcgaactcta tgcaatcgac gaccgtccca
     2761 accagttgta catggtcggc tcgatgggtt gcgtgacgcc gttcgcgttg ggtcttgcgt
     2821 tggcgcgtcc tgatctgcac gtggtcgcgc tcgacggcga cggcgccgcg ctgatgcgca
     2881 tgggcgcctt cgcgacgctc ggcgcgtacg gtccgtcgaa tctcacgcac gtgctgctcg
     2941 ataacggcgc gcacgattcg accggcggtc aggcgacggt gtcgccgcaa gtatcgtttg
     3001 cgggtgtcgc cgcggcgtgc ggttatgcgt cggcggttga aagcgacgac gtcaacgtaa
     3061 tcgacgcgtt gttcgaagct gcgccgtcgc cccatggccc gcgcttcgcg cgactgacga
     3121 ttcgccgcgg tacgcccgat ggcctgccgc gtcccactat caccccgccc gatgtgaaga
     3181 cgcgcctgat gcgtcacatc ggcgccgctt aaggagtgcg ctcatgttgc tgctcaatcc
     3241 cggcccggtg acgcttaccg aacgcgtccg caacagcctg ttgcagacgg atctgtgcca
     3301 tcgcgaaagc gaatttttcg acttgcagga cgaggcgcgc acgcgtctcg tcgatctgta
     3361 cggcctcgac gcaaatgaat ggcaagcggt cttgatgacc ggctcgggca ccgcggccgt
     3421 tgaaagcatg accgccgcgc tggtgccgca aaacggcaag ctgctggtcg tcgaaaatgg
     3481 cgtgtacggc gagcggattt cgcagatcgc cacgcaatac ggtatcgcgc atgagtcgct
     3541 gaaacacgac tggatgcagg cgccggatct cgcgaagctc gccgaacgcc tcgacgcgga
     3601 caaagcgttc acccatgtcg ccgtgattca tcacgaaacc acgacaggcc gtctgaacga
     3661 tctgcaggcg ctcggcgcgc tgtgccgtga acgcggtctg cgtctgctgg tggatggcgt
     3721 cagcagcttc ggcgccgaag cgatcgattt cgccgacgcc agtctcgacg ccgtcgcggc
     3781 gaccgcgaac aagtgtctgc atggcgtgcc cggcgcgtcg ttcgtgctgg tgcggcgcgc
     3841 ggcgctcgcg caggcggcga gccgcacgta ttacctggac ctcgggcgcc tcgcgcgttt
     3901 gcaggatcag cgcaatacgc cgttcacgcc gtcggtgcat gcgtactacg cgctggtcga
     3961 agcgctgcgc gaactcgccg acgaaggcgg ttggcaagcg cgccatgcgc gttatgccgc
     4021 gctcgccgag caggctcgcg cgggcctggc ggagcgggga atcggcagtg tgttggcgcc
     4081 ggagcagtca tcggttgtgc tgcgcgcgta tcgcttgccg gagggcgtgg cgtatccgcg
     4141 gctgcacgac gcgctgaagg cgcgcggttt cgtcatctat gccggtcaag gcggcttgtc
     4201 ggccgaa
//
LOCUS       DATASH010000913         1598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_7077807,
            whole genome shotgun sequence.
ACCESSION   DATASH010000913 DATASH010000000
VERSION     DATASH010000913.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7077807"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..1317)
                     /locus_tag="VHV99_24395"
     CDS             complement(109..1317)
                     /locus_tag="VHV99_24395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX3638560.1"
                     /translation="MSEANMSETKSDPIVIVGVARTPMAAFHGDFASLTAPQLGSVAI
                     EAAVQRAGLKPGQIDEVVMGCVLPAGLGQAPARQAALGAGLPLSTGSTTVNKMCGSGM
                     RAAMFAHDMLAAGSVDVIVAGGMESMTNAPYLLPKARNGMRMGHGQVIDHMFYDGLED
                     AYEKGRLMGTFAEECAASFDFTREAQDAFAVESLNRAKRANEDGSFAWEIAPVKVASR
                     KGDVTIDHDEQPFKANLDKIPTLKPAFSKTGSVTAANSSSISDGAAALVMMRESTAKR
                     LGVEPLARVVGHSTFAQEPAKFTTAPVGAIRKLFEKNGWRADEVDLYEVNEAFAVVTM
                     AAMKEHQLPHDKVNVNGGACALGHPIGASGARILVTLIGALKKRGLKRGVATLCIGGG
                     EATAMGIELV"
     gene            complement(1338..>1598)
                     /locus_tag="VHV99_24400"
     CDS             complement(1338..>1598)
                     /locus_tag="VHV99_24400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015001469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HEX3638561.1"
                     /translation="INDAWARGQELTVHGWVYGVHDGNVRNLGMTISDAAALDATYRN
                     CVAAVSASRAHNADNDMVAADAAQLGDVPAIVEGVIKELKHE"
BASE COUNT          273 a          545 c          463 g          317 t
ORIGIN      
        1 actgatgcgc aaactcctga ccgatgccgc gcgatgcacc gacgatcaac actgtcttca
       61 tcgaatcacc tcaagccctt aaaccctcaa acgcctgcaa acagcgcgtt aaaccagttc
      121 aatgcccatc gccgttgctt ccccgccgcc gatgcacagc gtcgcgacgc cgcgcttcag
      181 accgcgcttt ttcaacgcgc cgatcagcgt gacgagaatc cgcgcgcccg atgcgccgat
      241 cgggtggccg agcgcacaag cgccgccgtt cacgttgacc ttgtcatgcg gcaactgatg
      301 ctctttcatc gccgccatcg tgacgaccgc gaacgcttcg ttgacttcat acaggtccac
      361 ttcgtcggcg cgccagccgt tcttctcgaa cagcttgcga atcgctccga caggtgcagt
      421 ggtgaacttt gccggttctt gcgcgaaggt cgaatggccg accacacgag caagcggttc
      481 gacgccgaga cgtttcgcgg tcgattcgcg catcatcacg agcgccgctg cgccgtcgga
      541 aatcgacgac gagttcgccg ccgtcaccga gccggttttg ctgaacgcgg gcttgagcgt
      601 cggaatcttg tcgaggttcg ctttgaacgg ctgctcgtcg tgatcgatgg tcacgtcgcc
      661 cttgcggctt gccaccttca ccggtgcgat ttcccaggca aacgagccgt cttcattcgc
      721 gcgcttcgcc cgattcaggg actcgacggc gaacgcatcc tgcgcttcgc gcgtgaagtc
      781 gaacgacgcc gcgcattcct cggcgaacgt gcccatcaaa cggccctttt cgtaggcgtc
      841 ttcgagaccg tcatagaaca tgtggtcgat cacctggccg tggcccatgc gcatgccgtt
      901 acgcgccttc ggcaacaggt acggcgcgtt ggtcatgctc tccatgccgc cagcgacgat
      961 cacgtcgacc gagccggcgg ccagcatgtc gtgcgcgaac atcgccgcgc gcatgccgga
     1021 gccgcacatc ttgttgacgg tggtgctgcc ggtgctcaac ggcaagccgg ccccgagcgc
     1081 ggcctgacgc gcgggcgcct ggccgagacc cgcgggcaat acgcaaccca tcacgacttc
     1141 gtcgatttgc ccgggcttca gaccggcgcg ctgcaccgcg gcttcgattg caaccgagcc
     1201 caactgcggc gcggtcaacg aagcgaaatc gccatgaaat gccgccatcg gcgtgcgcgc
     1261 cacgcctacg attacgatcg gatcagactt tgtctcgctc atgtttgcct cactcatgcg
     1321 ggtttcattc atgcgggtta ttcgtgctta agttctttga tcacaccttc gacgatggcc
     1381 ggcacgtcgc cgagctgggc cgcgtcggcg gcgaccatat cgttgtccgc gttgtgcgcg
     1441 cggctcgccg acaccgccgc gacgcagttc ctgtaggttg catcgagtgc ggcagcgtcg
     1501 ctaatcgtca tgccgaggtt gcgcacgttg ccgtcgtgca cgccatacac ccagccatgc
     1561 acggtgagtt cctgaccgcg tgcccacgcg tcgttgat
//
LOCUS       DATASH010000914         2221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3121012,
            whole genome shotgun sequence.
ACCESSION   DATASH010000914 DATASH010000000
VERSION     DATASH010000914.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3121012"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1796)
                     /locus_tag="VHV99_24405"
     CDS             complement(<1..1796)
                     /locus_tag="VHV99_24405"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HEX3638562.1"
                     /translation="MVAVVASGCYFLAYASQASADTTAAADRQAVLSKYCFMCHNEKL
                     KSGGLALSTIDLANLGKNPEKWEKVVRKLRTGAMPPGKMPRPDKAMTETIVVGIETEL
                     DRAALDNPNPGRPSLQRLNRSEYRNAIRDLLALDIDAASMLPADAAGYGFDNNADALS
                     LSPALTERYLGAAAKISQMALSRPRGLPTPETFFVPTDRNQAVRVSDDLPFGSRGGLV
                     VHHYFPADGQYLFEMRPKESGVAGGFEGITAEVHQVEVRLDDVKVWSGTIGGQEFVHK
                     RGPNAGGATEEELNKKVLEGLKFHVPVKAGEHLIQAYFIAKTEAYVEDLFDPSIRREN
                     YRNPAGPPKLSTLTVTGPQSGTAAVSETPSRQRILLCAPQSAKDETCAKKIISTLARR
                     AYRRPVTEADLQAPLVSFRGGLARAGFESGIEMALRSILVSPNFLFRFEDQPAGLADH
                     APYRINDLELASRLSFFLWSSIPDDELLGVAENKSLHQPEVLKQQVHRMLADSRSQAL
                     VDNFAGQWLFIRNVTTHQPSPEILFHFDDNLRQAFEQETELFFNSIIRENRSVTDLLD
                     ANYTFLNQRLAEHYGIQGVRGERFRRVELPED"
     gene            complement(1805..>2221)
                     /locus_tag="VHV99_24410"
     CDS             complement(1805..>2221)
                     /locus_tag="VHV99_24410"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX3638563.1"
                     /translation="GVNITPLMAAAGISWASNQDRASEEQVLEAVKLLVEELGADVNF
                     VADTGETAMHAAAYRGANNVVQYLFDKGAKLDVVDKSGRTPLAIADGVEYGNSFAAQP
                     QTAELLRKLGAKEIPCPNLCPNLILEENLPPEEAKK"
BASE COUNT          406 a          672 c          678 g          465 t
ORIGIN      
        1 ctatcttcgg gaagctcgac gcgccggaat cgttcgccgc gcacgccttg gattccgtag
       61 tgttcggcca gccgctggtt caagaaagtg taattggcgt cgagcaggtc agtcacgctg
      121 cggttctcgc ggatgatgct attgaagaac aactccgttt cctgctcgaa ggcctggcgc
      181 aggttgtcgt cgaagtgaaa caggatctcc gggctcggct ggtgcgttgt cacgttgcgg
      241 atgaacagcc actggccggc gaagttgtcg accagcgcct gcgagcgcga atccgccagc
      301 atccgatgca cctgctgctt caagacctcg ggctgatgga ggcttttgtt ttcggcgacg
      361 ccgagcagtt cgtcatccgg gatactgctc cacaggaaga aggagagtcg cgatgccaat
      421 tccaggtcgt tgatgcgata cggcgcatgg tcggccagcc ccgcgggctg gtcttcgaaa
      481 cggaacagga agttcgggct caccagaata cttcgcaggg ccatctcgat gccggattca
      541 aatccggcac gcgccaaacc gccgcggaag gaaaccagcg gggcctgaag atcggcttcc
      601 gtcaccgggc ggcggtacgc acgccgtgcc agcgtcgaaa taatcttctt cgcgcacgtc
      661 tcatccttcg ccgactgagg ggcgcagagc aaaattcgtt gccggctcgg cgtttccgag
      721 accgctgccg ttccggattg cggaccggtg accgtcagcg tcgaaagctt aggcggccca
      781 gccggattgc ggtagttttc gcggcgtatg gacggatcga aaaggtcttc gacgtatgct
      841 tcggtcttag cgatgaaata cgcttgaatc aaatgctcac cggccttcac cggcacgtgg
      901 aactttaacc cctccaggac tttcttgttg agttcctctt ccgtcgcccc gcccgcattc
      961 ggtccacgct tatgcacgaa ctcttgcccg ccgatcgtgc ctgaccagac cttgacatcg
     1021 tccagcctga cctcgacttg atgcacttct gccgtgatgc cctcgaaccc gccggcaacg
     1081 ccgctctctt tgggacgcat ttcaaacaag tactggccgt cggccggaaa atagtgatgc
     1141 accaccagcc cgccgcgcga tccaaagggc agatcgtcac tgacgcggac ggcttggttg
     1201 cgatcggtcg gcacgaagaa agtttcggga gtcggcaagc cgcgcggacg cgagagcgcc
     1261 atctggctga tcttcgccgc cgcgccgaga taacgctcgg tcagtgccgg cgatagcgac
     1321 agggcgtcag cgttattatc gaacccgtag cccgcggcgt cggccggcag catcgaagcg
     1381 gcgtcgatat cgagggccaa cagatcgcgg atggcattgc ggtattcgga gcggttcagg
     1441 cgttgcagcg acgggcgccc tggattggga ttgtcgagcg cagcccggtc cagctctgtt
     1501 tcgatgccaa cgacaatggt ctcggtcata gccttgtccg gccgcggcat tttgccggga
     1561 ggcatagcgc cagtccggag cttgcgaact actttctccc acttctcggg attcttgccc
     1621 aagttggcta ggtcgatcgt gctgagtgca aggccgccag actttaattt ctcgttgtgg
     1681 cacatgaagc agtacttact gagaacggcc tgacggtcgg cagcggccgt tgtgtcggcg
     1741 gatgcctgtg atgcgtaggc gagaaagtaa caaccagagg cgacgaccgc gaccaaacga
     1801 ggtctcattt cttagcctcc tcgggcggca agttttcttc caggatcaag ttcgggcaca
     1861 gattcggaca cggtatttct ttcgccccca gcttacgcag cagctcggcc gtctgcggct
     1921 gggcggcgaa tgaattgccg tactcgactc cgtcggctat ggccaatggc gttcgcccgc
     1981 ttttgtcgac tacgtcgagc ttcgcgcctt tgtcgaagag atactgcacc acgttgttgg
     2041 cgccgcggta agcggccgcg tgcatcgctg tctcgccggt gtcggcgacg aagttgacgt
     2101 cggcgcccag ttcttctacc agcaacttga ccgcttccag cacctgttcc tcgctggccc
     2161 ggtcttgatt ggaggcccac gagatgcccg cggcagccat caacggcgtg atgttgacgc
     2221 c
//
LOCUS       DATASH010000915        11338 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4902110,
            whole genome shotgun sequence.
ACCESSION   DATASH010000915 DATASH010000000
VERSION     DATASH010000915.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 11338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11338
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4902110"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="VHV99_24415"
     CDS             complement(<1..298)
                     /locus_tag="VHV99_24415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011352307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX3638564.1"
                     /translation="MNAKKVLILGVNGFIGHHLSKRILETTDWEVFGMDMQTERLGDL
                     VNHERMHFFEGDITINKEWVEYHIKKCDVILPLVAIATPATYVKQPLRVFELDFE"
     gene            complement(295..1230)
                     /locus_tag="VHV99_24420"
     CDS             complement(295..1230)
                     /locus_tag="VHV99_24420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase"
                     /protein_id="HEX3638565.1"
                     /translation="MKPRAVVFAYHNVGVRCLQVLLARGVDVALVVTHEDSPTENIWF
                     GSVASVAAGYGIKVVTPADPKSAELREALSAARPDFIFSFYYRHMLPVDLLALAARGA
                     YNMHGSLLPKYRGRVPTNWAVLHGETETGATLHEMAAKPDAGAIIAQTPVPILPDDTA
                     AQVFDKVTVAAEQTLWRVLPALLAGVAPHLPNDLSHGSYYGGRKPEDGRIDWTQTAQQ
                     VYNLIRAVAPPYPGAFADVEGHRFIIARARLAAPGALRSDLPPGLHVSDNAVFAICGD
                     GRAITIHELRHQHDGTETVATPAEFAQLIQIPRHS"
     gene            complement(1227..2270)
                     /locus_tag="VHV99_24425"
     CDS             complement(1227..2270)
                     /locus_tag="VHV99_24425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3638566.1"
                     /translation="MSYSEHRAVTPEVSIIIPVYNEEAGLAALFARLYPALDALGTAY
                     EVIFINDGSRDKSAALLAEQFRARPDTTRVILLNGNYGQHMAILAGFEQSRGEIVITL
                     DADLQNPPEEISKLVNKMREGYDYVGTIRLQRQDSLWRRKASRAMNRLRERITRIKMT
                     DQGCMLRAYSRYIIDTINRCGEINTFIPALAYTFAQNPVEIEVAHEERFAGESKYSLY
                     SLIRLNFDLVTGFSVVPLQWLSFIGVILSLGSAALFVLLLIRRFIIGAEVQGVFTLFA
                     VIFFLLGVIIFALGLLGEYIGRIYQQVRARPRYLVQTILEQRDGTTVTEAPRQAVVQA
                     VQAAPLIDQGPNP"
     gene            complement(2277..3428)
                     /locus_tag="VHV99_24430"
     CDS             complement(2277..3428)
                     /locus_tag="VHV99_24430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006496385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEX3638567.1"
                     /translation="MSQSTVPFLPFVKPEIDEETIQGVADVLRSGWITTGPQNQKFEA
                     ALSEFCGGRPVRTFNSGTATLEIGLRIAGVGEGDEVITTPATWVSTSNVIYEVGATPV
                     FADIDPVTRNIDLDLLEKAITPRTKAIIPVYLSGLPVDLDRLYEIARAHKLRVIEDAA
                     QAFGSTWKGERIGKLGDIVSFSFHANKNLTSIEGGALVLNNEEEAILAQKYRLQGITR
                     TGFDGMDCDVLGGKYNLTDVAARVGLGQLPHLERFLAQRKKLVRAYFAGLEGGAAVKL
                     GVGLPFADFENSNWHMFQITLPLEKLSIDRAGFMGQLKERGIGSGVHYPAIHLFSLYH
                     TKGFREGMFPHAERFGATTVTLPLFTLMNEGDVERVCRAVNEICEQYGK"
     gene            complement(3691..4062)
                     /locus_tag="VHV99_24435"
     CDS             complement(3691..4062)
                     /locus_tag="VHV99_24435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006482863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HEX3638568.1"
                     /translation="MNPISLFCILAGVTLNAGAQLLLKAGTNAVGHFEFTRANILPIA
                     FRLATQPPIIGGLACYVISVGVWVIGLSRVDVSIAYPMLSLGYVVNAFAAWYLFGEVM
                     SVQKLVGIGVILIGVVVLARS"
     gene            complement(4088..5836)
                     /locus_tag="VHV99_24440"
     CDS             complement(4088..5836)
                     /locus_tag="VHV99_24440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX3638569.1"
                     /translation="MNDTPTRLPLNRTTLLLLVLALAVIWFVPLGWRHLLPSDEGRYA
                     EMAREMFVTGDWITPRYNGYKYFEKPPLQTWANALTFAWFGVGEWQARLYTALTGFAG
                     VLLIGFTGARVFNVATGVFAAIVLATSPYWNLMGHFNTLDMGLSFWMELTLCALLLAQ
                     RPKLPSANVRAWMWVCWGSMALAVLSKGLVGVILPGAVLVLYSLLARDWALWKRLHLI
                     GGLIVFFAIVTPWFVLVQQRNPEFLNFFFIVQQFKRYLTPEQNRPGPFYYFVPVLLVG
                     FLPWLSVTFQSVRHAWRLPRQPNGFAPVTLMLVWTAFIFLFFSASHSKLLSYTLPIAP
                     PIALVIGMYLPLVTRDQLRRHLAGYALFLVVAAFAALFMSRLGNARNPTELYVEYRTW
                     VLAALGVAFVLTLVALWLNRRSRAGTLGAVASFGAAWLLLGTIAGTGHDVFGRLSSGA
                     PLAPAIKAEIAKLPADTPFYSVGVLDHTLPFYVDHTTIMVEHPDELAFGVSVEPQKWV
                     PSVDAWIERWKAERYALALIPPPTYDRLLKEGLPMQVIARDPRRVVVMKPVAVPAPAS
                     GAAAPAAPAAVEKPQQ"
     gene            complement(6120..6494)
                     /locus_tag="VHV99_24445"
     CDS             complement(6120..6494)
                     /locus_tag="VHV99_24445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HEX3638570.1"
                     /translation="MKLHQDSSGALNTVTGYGADYVEINLVRHSGSILVLPEAPVIPW
                     PVSSFDDLSAEHFAMLVESAPEVVVFGSGERLRFPHPRLTAALTAKRIGVETMDFKAA
                     CRTYNILMAEGRKVAAALLIEA"
     gene            7022..8269
                     /locus_tag="VHV99_24450"
     CDS             7022..8269
                     /locus_tag="VHV99_24450"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018432089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX3638571.1"
                     /translation="MPAVKPILKSNKLLNVCYDIRGPVLEHAKRLEEEGHRIIKLNIG
                     NLAPFGFDAPDEIIQDMILNLPGSSGYSDSKGVFAARKAIMHYTQQKGVHGVELDDIY
                     IGNGASELIVMALQGLLNDGDEVLLPAPDYPLWTAGVSLSGGTPVHYICDESNSWMPD
                     LDDIRAKITPNTRALVVINPNNPTGALYSDELLLGLIEIARQHGLVIFADEVYDKIVY
                     DGKKHTSMAALSEDVLTVTFNSLSKSYRSCGYRAGWMFISGLTGENRRNAKDYFEGLG
                     ILASMRLCPNVPGQYAIQTALGGYQSINDLIVPSGRLYKQRELAYDMLTAIPGVSCVK
                     PEAALYMFPRLDPKVYPIQDDQQFILDLLLEERVLLVQGTGFNWKTPDHFRVVFLPNV
                     DDLADSINRIARFLDGYRKRHTA"
     gene            8346..9632
                     /locus_tag="VHV99_24455"
     CDS             8346..9632
                     /locus_tag="VHV99_24455"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008349813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEX3638572.1"
                     /translation="MEPIKVGLLGFGTVGSGTFTVLRRNQEEIKRRAGRGIEIARIAV
                     RNPAKATVALGSAAGTVALTDDFNAVVDDPSIDIVAEMIGGTGVARDLVLRAIKNRKH
                     VVTANKALLAVHGTEIFEAARANGVMVSFEAAVAGGIPIIKALREGLTANRIQYIAGI
                     INGTTNYILSEMRDRGLDFATALKAAQELGYAEADPTFDIEGVDAAHKATIMSAIAFG
                     VPVQFDKAYVEGISKLAAIDIKYAEELGYRIKLLGIARRTDKGIELRVHPTLIPEKRL
                     LANVEGAMNAVVVHGDAVGTTLYYGKGAGAEPTASAVVADLVDVTRLHTADPEHRVPH
                     LAFQPDSLSSTPILPIDEVTSGYYLRLRVADVTGVLADITRILADRGISIDALLQKES
                     EQVDANGKGETDIILITHETVEKNVNAAIKTIEALN"
     gene            9641..11092
                     /gene="thrC"
                     /locus_tag="VHV99_24460"
     CDS             9641..11092
                     /gene="thrC"
                     /locus_tag="VHV99_24460"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018418155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX3638573.1"
                     /translation="MNYLSTRGAGAGERHTFSDILLGGLAKDGGLYLPAEYPRVTADE
                     LTRWRALPYADLAFEILSKFSDDIPAEDLRALTRKTYTAQTYCNVRDDEGAAQITPLK
                     TLGVENGTTLSLLELSNGPTLAFKDMAMQLIGNLFEYALARHGETLNILGATSGDTGS
                     AAEYAMRGKQGISVFMLSPHKKMSAFQTAQMYSLQDPNIFNIAIEGVFDDAQDIVKAV
                     SNDHAFKAKYKIGTVNSINWARVVAQVVYYFKGYFAATKSNDERVSFTVPSGNFGNVC
                     AGHIARMMGLPIEKLVVATNENDVLDEFFRTGIYRVRKSAETYHTTSPSMDISKASNF
                     ERFVFDLLDRDPARVLQLFRDVEEKGGFDLAASGDFARVKAFGFVSGRSSHEDRVDAI
                     RDVFERYDTMIDTHTADGLKVAREHLQAGIPMIVLETAQPIKFGETIREALLREPERP
                     AAFSGLESLPQRFEVLPADVQRVKDYIVANTGE"
BASE COUNT         2203 a         3485 c         3643 g         2007 t
ORIGIN      
        1 cctcgaagtc gagttcgaac acgcgcagcg gttgcttaac gtacgtggcg ggcgtcgcga
       61 tcgcgaccag cggcaggatc acatcgcact tcttgatgtg atactcgacc cactccttgt
      121 tgatcgtgat gtcgccttca aagaagtgca tccgctcatg attgaccagg tcgcccagac
      181 gttcggtctg catgtccatc ccgaagactt cccaatcggt cgtttcgaga atgcgtttgg
      241 acaggtgatg gccgataaag ccgttcacac ccagaatcag gactttttta gcgttcatga
      301 atgacgggga atttggatga gctgggcgaa ttcggcgggg gtggcaacgg tttcggtgcc
      361 gtcgtgctga tgccgcaatt cgtggatggt gatcgcgcga ccgtcgccgc atatcgcgaa
      421 aacggcatta tcgcttacgt gcaggcccgg cggcaaatcc gagcgaagcg cgccgggcgc
      481 ggcgagacgc gcccgggcga tgatgaaacg gtggccttcg acgtcggcaa acgcgccggg
      541 atagggcggc gcgaccgcgc ggatcaggtt gtagacctgt tgcgcggtct gcgtccagtc
      601 gatccggccg tcttccggct tgcgcccgcc gtagtagctg ccgtgcgaga ggtcgttcgg
      661 caggtgcggt gcaacgcctg ccagcaaagc cggcagcaca cgccacagcg tctgctcggc
      721 ggccaccgtg accttgtcga acacctgcgc ggcggtgtcg tcgggcagga tcggcaccgg
      781 cgtttgcgcg atgatcgcgc ccgcgtcggg cttggccgcc atttcgtgca gcgtcgcgcc
      841 ggtttcggtt tcgccgtgga gcaccgccca gttggtcggc acgcggccgc ggtacttcgg
      901 cagcagcgag ccgtgcatgt tgtaagcgcc ccgtgcggcg agcgccagca ggtcgaccgg
      961 caacatgtgg cggtagtaaa aagagaagat gaagtccggc cgtgcagcgc tgagcgcctc
     1021 gcgcaattcc gcgctcttcg gatcggccgg cgtgacgacc ttgatgccgt acccggccgc
     1081 aaccgaggcg acgctgccga accagatgtt ctcggttggg ctgtcttcgt gcgtgacgac
     1141 cagcgccacg tcgacgccgc gcgcgagcag cacctgcagg cagcgcacac cgacgttgtg
     1201 atacgcgaat acgacggcgc gcggcttcat ggattcggcc cctgatcgat tagcggcgcg
     1261 gcttgcacgg cctgtaccac cgcctgacgc ggcgcttcgg tcacggtggt gccgtcgcgc
     1321 tgttcgagaa tcgtctgcac cagataacgc ggacgggcgc gcacctgctg gtaaatccgt
     1381 ccgatgtatt cacccagcag gcccaacgcg aagatgatca cgccgagcag gaagaagatg
     1441 acagcgaaca gcgtgaacac accttgcact tccgcgccga tgatgaagcg gcgaatcagc
     1501 agcagcacga acaacgcggc ggagccgagc gacaggatca cgccgatgaa cgacagccat
     1561 tgcagcggca ccaccgagaa gccggtgacc aggtcgaaat tcaggcggat caggctgtag
     1621 agcgagtact tcgattcgcc ggcaaaacgc tcttcgtgcg cgacttcgat ctcgaccggg
     1681 ttctgcgcga aggtgtacgc gagcgccgga atgaaggtgt tgatttcgcc gcagcgattg
     1741 atcgtgtcga tgatgtaccg gctgtacgcg cgcagcatgc agccctggtc ggtcatcttg
     1801 atgcgggtga tgcgctcgcg cagacggttc atcgcgcgtg aggccttgcg gcgccacagg
     1861 ctgtcctggc gttgcaggcg aatcgtgccg acgtagtcgt aaccttcgcg catcttgttg
     1921 accagcttgc tgatttcttc cggcgggttc tgcagatcgg cgtcgagcgt gatgacgatc
     1981 tcgccgcgcg attgttcgaa acccgccaga atcgccatgt gctggccgta gttgccgttc
     2041 agcagaatca cgcgggtcgt gtcgggccgc gcgcggaact gctcggcgag cagggcggcg
     2101 gatttgtcgc ggctgccgtc gttgatgaag atcacttcat aggcggtgcc gagcgcgtcg
     2161 agcgcggggt aaaggcgcgc gaacagcgcg gccagcccgg cttcctcgtt gtacaccggg
     2221 atgatgatcg acacttccgg tgtgacggcg cgatgttccg aataactcat ttccgcttat
     2281 tttccgtatt gttcgcaaat ttcgttgacc gcgcggcaga cccgttccac gtcgccttcg
     2341 ttcatcagcg tgaagagcgg caacgtgacg gtggtggcgc cgaagcgctc cgcgtgcggg
     2401 aacatgcctt ccctgaaacc tttcgtgtga tagagcgaga acagatggat cgccgggtaa
     2461 tgcacgcccg aaccgatgcc gcgttccttc aactggccca tgaaacccgc gcggtcgatc
     2521 gagagctttt cgagcggcag cgtaatctgg aacatgtgcc agttgctgtt ctcgaagtcc
     2581 gcaaacggca gacctacccc cagtttcacc gcggcgccgc cttcaaggcc ggcgaaatac
     2641 gcgcgcacga gctttttgcg ctgtgcgagg aaacgttcga gatgcggcaa ctggccgagg
     2701 ccgacgcgcg cggcgacgtc cgtcaggttg tacttgccgc ccagcacgtc gcagtccatg
     2761 ccgtcaaaac ccgtacgggt gatgccttgc aggcggtatt tctgggcgag gatcgcttcc
     2821 tcctcgttgt tcagcaccag cgcgccgcct tcgatcgagg tcagattctt gttcgcgtgg
     2881 aagctgaacg agacgatatc gccgagcttg ccgatgcgct cgcctttcca cgtcgagccg
     2941 aatgcctggg ccgcgtcttc gatcacgcgc agtttgtggg cgcgggcgat ctcgtacaga
     3001 cggtccaggt cgaccggcag accggacaaa tacaccggaa tgatcgcctt ggtgcgcggc
     3061 gtgatggcct tttcgagcaa atcgaggtcg atgttgcgcg tgaccgggtc gatgtcggca
     3121 aacacgggcg tggcgccgac ttcgtagatc acgttgctgg tcgacaccca cgtggcgggg
     3181 gtggtgatga cttcgtcgcc ttcgcccaca ccggcgatcc gcagcccgat ttcgagcgtc
     3241 gcggtgccgg aattgaacgt acgcaccggc cggccgccgc agaactcgga caacgccgct
     3301 tcgaacttct ggttctgcgg gccggtcgtg atccaaccgg agcgaagcac gtcggcgacg
     3361 ccctgaatcg tttcttcatc gatctcaggt ttgacaaacg gcaaaaacgg gactgttgac
     3421 tggctcatga atgcttcgct cgattgaaag gggcgcaacc cgaagggcgc gaagtagatc
     3481 acaaaccact cgcaaaccca aggccacgac taaaaaaagg cgcaaccgcc aagcctaccg
     3541 cgcctaacct tacgttttac ttagccggcg gcggtgcccg gcttcccaga tcgctggcag
     3601 ccgggatttt cgcagcggct gcacccgcac ccgcaccggc aataaacggt aataagccgt
     3661 gcccggcacg gcacggccgg ggcatacgac ctagctgcgc gccaggacga cgacgccgat
     3721 caggatgacg ccgatcccga ccagcttctg gactgacatc acttcgccga atagatacca
     3781 cgccgcgaac gcgttgacga cgtatccgag cgacagcatc gggtaggcaa tcgacacgtc
     3841 cacccgcgag agcccgatca cccaaacgcc cacgctgatc acgtagcagg ccagcccgcc
     3901 gatgatcggc ggctgggtcg ccaggcgaaa ggcgatgggc aggatgttcg cacgggtgaa
     3961 ttcgaagtgt ccaacggcat tcgttccggc tttgagcaac aactgcgcgc cggcgttcaa
     4021 cgtaacgccg gcgagaatgc aaaagaggga aatcgggttc atcgaacggt tagatcctga
     4081 gatggggtca ttgttgtggt ttttctactg ccgccggtgc cgctggtgcc gctgcgccgg
     4141 atgcgggcgc gggcaccgcc acgggcttca tcaccaccac ccggcgcggg tcgcgcgcga
     4201 tcacctgcat cggcagcccc tctttgagca gcctgtcgta agtcggcggc gggatcagcg
     4261 cgagcgcgta gcgctcggcc ttccagcgtt cgatccacgc gtcgaccgac ggcacccatt
     4321 tctgcggttc gacggatacg ccgaatgcca gttcgtcggg atgttcgacc atgatcgtcg
     4381 tgtggtcgac atagaacggc agcgtatggt cgagtacgcc gaccgagtag aacggcgtat
     4441 cggccggcaa ctttgcgatt tcggccttga tggcgggcgc gagcggcgcg cccgaactca
     4501 aacggccgaa cacgtcgtga cccgtgccgg caatcgtacc gagcagcagc caggccgcgc
     4561 cgaagctcgc cacggcgccc agcgtgccgg cgcggctgcg gcggttcagc cacagcgcga
     4621 ccagcgtgag cacgaaggcg acgccgagtg ccgccagcac ccatgtgcgg tactcgacat
     4681 atagctcggt cgggttgcgc gcgttgccca ggcgcgacat gaacagcgcg gcgaacgcgg
     4741 cgaccacgag aaacagcgca tagccggcca ggtggcggcg cagttgatca cgtgtgacga
     4801 gcggcagata catgccgatg acaagggcga tcggcggcgc gatcggcagc gtgtaggaca
     4861 ggagcttcga gtgcgacgcg ctgaagaaca ggaagatgaa cgcggtccac accagcatca
     4921 gcgtgaccgg cgcgaaaccg ttcggctggc gcggcaggcg ccacgcgtgg cgcacgctct
     4981 ggaaggtcac cgacaaccac ggcaggaagc cgaccagcag caccggcacg aaatagtaga
     5041 acggccccgg gcggttctgt tcaggcgtca gataacgctt gaactgctga acaatgaaga
     5101 agaagttcag gaattccggg ttgcgctgct gtaccagcac gaaccatggc gtgacgatcg
     5161 cgaagaacac gatcaggccg ccgatcagat gcaggcgctt ccacagggcc cagtcgcggg
     5221 cgagcaggct gtacagcacc agcaccgcac ccggcaggat cacaccgacg agacccttgg
     5281 agagcaccgc gagcgccatc gagccccagc acacccacat ccatgcccgc acgttggcgc
     5341 tcggcagttt ggggcgctgc gccagcagca gcgcgcacag cgtcagctcc atccagaacg
     5401 acaggcccat gtcgagcgtg ttgaagtggc ccatcaggtt ccagtacggc gacgtggcca
     5461 gcacgatcgc cgcgaatacg ccggtcgcca cgttgaacac gcgcgcgccg gtgaagccga
     5521 tcagcagcac gccggcaaag cccgtcagcg cggtgtaaag ccgcgcctgc cattcgccga
     5581 cgccgaacca cgcgaacgtg agcgcattcg cccaggtttg cagcggcggt ttctcgaagt
     5641 acttgtagcc gttatagcgc ggcgtgatcc agtcgccggt gacgaacatc tcgcgcgcca
     5701 tttcggcgta gcgcccctcg tcgctcggca gcagatggcg ccagccgagc ggcacgaacc
     5761 agatcacggc gagtgccagc accagcagca ggagcgtggt gcgattgagc ggtagccttg
     5821 tcggcgtatc gttcatgtat ttcaaccttt tgggcgacgc caggtgcggc gtggcagtgt
     5881 tgtatgaaat tgaggacggc gctccggcag cctgctttcc ggagatgcaa cattcattcg
     5941 gacgctaacc ggaccacgct gctttggggc gccgccaaag cgccaatggc gcgcagtgta
     6001 cgttatcggc cgtgacgcgc ccagggcgcg cagtcggttc gtgtcgctgg gcacggcgcg
     6061 agtccccgga tcacgagcat gcgtgcgcag cccgcatggg tgcggcgccc gccaggccgt
     6121 tacgcttcga tcagcaacgc ggctgcgacc ttgcggccct cagccatcag aatgttgtag
     6181 gtgcggcagg cggccttgaa gtccattgtt tcgacgccga tgcgtttggc ggtgagcgcg
     6241 gcggtcaggc gcggatgcgg aaagcgcagc cgttcgccgc tgccgaacac caccacttcg
     6301 ggcgccgatt ccaccagcat ggcgaagtgc tcggcgctca ggtcgtcgaa agaagagacg
     6361 ggccaaggga tgacgggcgc ttccggcagc acgagaatgc tgcccgaatg gcgcaccaga
     6421 ttgatttcga catagtcggc gccgtagccg gtaacggtgt tgagggcgcc gctggagtcc
     6481 tgatgtaatt tcaaattcgt tttccgaagt cgtcgtgagg catcatgcgt gtgggcggcc
     6541 tgaccgggca gaacggaagc agggcggttt gacgcgagtg ggacacgcga gtggcacaga
     6601 agcggccgaa aaggcttgcc gttgcggttg gtgcattgcg gaagtcggcc aaaatccgct
     6661 aaattataac tttttagccg ccttgcggcg cccccgctca agtgctgcct catgtgcggt
     6721 cataagccgc gctgagggcg cggacgcgac ccaccggcta cctgcccggt tctgcacgtt
     6781 ttcggctgtt ttcggtctgc tgcttttggt ctgctttggc ctgcctggag cggtctggtt
     6841 gccgcaccgg cgcaatcggc cgcagcgcgc ccgactggcc cggaaagccg gaactccgcg
     6901 tcttgcggcc cgatccgtgt ctggcggctg cgccgcgtca taatcccccg tatcggcgga
     6961 cttgcccggg gtcccgcttt ttcacccccg cttgttcgcc caagcccatc aacaggatga
     7021 agtgcccgcc gtgaaaccga ttctcaaatc caacaagttg ttgaatgtct gctacgacat
     7081 tcgcgggcct gtcctcgaac atgcaaagcg gctcgaagaa gagggccacc gcatcatcaa
     7141 gctgaacatc ggcaatctcg cgccgttcgg cttcgacgcg ccggatgaaa tcatccagga
     7201 catgatcctg aatctgccgg gttcgtccgg ctactcggat tccaagggtg tgttcgccgc
     7261 gcgcaaggcg atcatgcatt acacgcagca aaagggcgta catggcgtcg aactggacga
     7321 catctatatc ggcaatggcg cctccgagct gatcgtgatg gcgctgcagg gtctgctgaa
     7381 cgacggcgac gaagtgttgc tgcccgcgcc ggactacccg ctgtggaccg caggcgtaag
     7441 cctttccggc ggcacgcccg tacactacat ctgcgacgaa tcgaacagct ggatgcctga
     7501 tctggacgac atccgtgcaa aaattacgcc gaatacgcgt gcgctcgtcg tcatcaatcc
     7561 gaataacccc accggcgcgc tgtactcgga cgaactgctg ctcggtctga tcgagatcgc
     7621 ccgccagcac ggcctcgtga tcttcgccga cgaggtctac gacaagatcg tctacgacgg
     7681 caagaagcac acgtcgatgg ccgcgctctc cgaggacgtg ctcaccgtca ccttcaacag
     7741 cctctcgaag agctaccgtt cgtgcggcta ccgcgccggc tggatgttca tttcgggcct
     7801 gaccggcgaa aatcgccgca atgccaaaga ctacttcgaa gggctcggca ttctggcctc
     7861 gatgcgcctg tgcccgaacg tgcccggcca gtacgcgatc cagaccgcgc tcggcggcta
     7921 tcaaagcatc aacgacctga tcgtgccgag cgggcgcctg tacaaacagc gcgaactcgc
     7981 gtatgacatg ctgacggcca tccccggcgt gagctgcgtg aagccggagg cggcgctgta
     8041 catgttcccg cgcctcgatc cgaaggttta tccgatccag gacgaccagc agttcatcct
     8101 cgaccttctg ctggaagagc gcgtgctgct ggttcaaggc accggattca actggaagac
     8161 gccggatcac ttccgcgtcg tgttcctgcc gaacgtggac gacctcgcgg attcgatcaa
     8221 ccggatcgcg cgtttcctcg acggctaccg caaacgccac acggcttgag atggacggcg
     8281 cgcctcgcgc gcgccgctgc cagccccagt ttccttttaa tcatttactc gaaaacacac
     8341 gctgcatgga accgatcaaa gttggactgc tgggcttcgg cacggtaggc agcggcacct
     8401 tcacggtact gcgccgcaat caggaagaaa tcaaacgtcg cgcgggccgc ggcatcgaga
     8461 ttgcgcgcat tgccgtgcgc aaccccgcca aggcgaccgt ggcgctcggg agtgctgccg
     8521 gcaccgtggc gctgaccgat gacttcaacg cggtggtgga cgatccgtcg atcgatatcg
     8581 tggccgaaat gatcggcggc acgggcgtgg cacgcgacct cgtgctgcgc gcgatcaaaa
     8641 accgcaagca tgtggtcacc gccaacaagg cgctgctcgc ggtgcacggc acggagattt
     8701 tcgaagcggc gcgcgccaac ggcgtgatgg tgtcgttcga agcggcggtg gcgggcggca
     8761 ttccgatcat caaggcgctg cgcgaagggc tcacggccaa tcgcatccag tacattgccg
     8821 gcatcatcaa cggcacgacg aactacatcc tctcggagat gcgcgaccgt gggctcgact
     8881 tcgcgaccgc gttgaaagcc gcgcaggaac tgggttacgc cgaagccgat ccgaccttcg
     8941 acatcgaagg cgtggacgcc gcgcacaagg cgacgatcat gagcgcgatc gcgttcggcg
     9001 tgccggtgca gttcgacaag gcgtacgtcg aaggtatcag caagctcgcg gcgatcgaca
     9061 tcaaatacgc cgaggaactc ggctaccgca tcaagttgct cggtattgcg cgccgcacgg
     9121 ataagggcat cgaactgcgc gtgcatccca cgctgattcc tgaaaagcgc ctgctggcga
     9181 acgtggaagg cgcgatgaac gcagtcgtcg tgcatggcga cgccgtgggc accacgctgt
     9241 actacggcaa gggcgcgggc gctgagccga cggcgtccgc ggtggtggcc gacctggtcg
     9301 acgtgacgcg tctgcatacc gcggatccgg agcatcgcgt gccgcatctg gcgttccagc
     9361 cggacagcct gtcgagcacg ccgatcctgc cgatcgacga agtgacgagc ggctactacc
     9421 tgcgtctgcg cgtggcggac gtgaccggcg tgctggccga catcacacgt attctggcgg
     9481 ataggggcat ctcgatcgac gcactgctgc agaaggaatc ggagcaggtc gacgcgaacg
     9541 gcaagggcga aaccgacatc atcctgatta cgcacgaaac ggttgagaag aacgtcaacg
     9601 cggcgatcaa gacgatcgaa gcgctgaact aggccgaact atgaactatc tctctacgcg
     9661 cggcgccgga gccggcgagc gccatacatt ttccgacatt ctgctgggtg gtcttgcgaa
     9721 agatggcggc ctgtacttgc cggcggaata tccgcgcgtc acagctgacg agttgacgcg
     9781 ctggcgcgcg ctgccctacg cggacctcgc gttcgaaatc ctgtcgaaat tcagcgacga
     9841 cattcccgcc gaagacctgc gcgcgctgac gcgcaagacc tatacggccc agacgtattg
     9901 caatgtgcgt gacgacgaag gcgccgcgca gatcacgccg ttgaagacgc ttggcgtcga
     9961 gaacggcaca acgttgtcgc tgctggaact gtcgaacggc ccgacgcttg cgttcaagga
    10021 catggcgatg cagttgatcg gcaacctgtt cgaatacgcg ctggcgaggc acggcgaaac
    10081 cctgaacatt ctcggcgcga cttccggcga caccggcagc gcggctgaat atgcgatgcg
    10141 cggcaaacag ggcatcagcg tgttcatgct ctcgccgcac aagaagatga gcgcgttcca
    10201 gaccgcgcag atgtatagcc tgcaggaccc gaacatcttc aacatcgcga tcgagggcgt
    10261 gttcgacgac gctcaggaca tcgtcaaggc ggtctcgaac gatcacgcgt tcaaggcgaa
    10321 gtacaagatc ggcacggtca actcgatcaa ctgggcacgc gtcgtggcgc aggtcgtcta
    10381 ctacttcaag ggctactttg ccgcgacgaa gtcgaacgac gagcgtgtgt cgttcacggt
    10441 gccgtcgggc aatttcggca atgtgtgcgc gggtcacatc gcgcggatga tgggtttgcc
    10501 gattgagaag ctggtggtgg cgacgaacga gaacgacgtg ctcgacgagt tcttccgcac
    10561 gggcatttac cgtgtgcgca agtcggcgga gacgtatcac acgaccagcc cgagcatgga
    10621 catttcgaag gcttcgaact tcgagcgttt cgtattcgat ctgctggatc gcgatccggc
    10681 gcgcgtgttg caactgttcc gcgacgttga ggagaagggc ggattcgacc tcgcggcgag
    10741 cggcgatttt gcgcgcgtga aggcgtttgg tttcgtatcg gggcgcagca gccacgagga
    10801 ccgggtggac gcgatccgcg acgtctttga gcgttacgac acgatgatcg acacgcacac
    10861 ggcagacggc ctgaaggtgg cacgcgagca tttgcaagcg ggcatcccga tgatcgtgct
    10921 ggagacggcg caaccgatca agtttggcga aacgatccgc gaagcgctgc tacgcgaacc
    10981 ggagcgccca gccgcgtttt cgggtctgga atcgctgccg cagcgattcg aagtattgcc
    11041 ggcagacgtt cagcgcgtga aggactacat cgtcgcgaat acgggcgagt aaacggcaag
    11101 tatggagcgc gccagagtcg cgctccatac gaccagcgcg ccagtttcag gaaataccgc
    11161 gacggcgcgc gcagcgccgc cggaagtatg acggctgtgt cactcacgcg gagtgtgcga
    11221 gaacacagat tccagatgca ccactaccgt ggctgtgcgg gggacccgct tatgagctgg
    11281 cggtttgagc cgctttcttt tgcctacttt tctttgcggc agggtaacta ttcagcgg
//
LOCUS       DATASH010000916         1659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1145722,
            whole genome shotgun sequence.
ACCESSION   DATASH010000916 DATASH010000000
VERSION     DATASH010000916.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145722"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            327..1418
                     /locus_tag="VHV99_24465"
     CDS             327..1418
                     /locus_tag="VHV99_24465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010091768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX3638574.1"
                     /translation="MRAGAALLLAAAALPALAALPIGRIKLPPGFHIEVLSDAVPTAR
                     AMALSPKGILYIGSLDGHVYALELQNGHVTARHVIVSGLETPAGVAWRDGTLYVSAVS
                     NILRFDAIDTHLSDPPKPAIVTDKLPTETHHGWKFIAFGPDGKLYVPQGAPCNVCLKD
                     RDRFGLIGRMDPDGSHYEVVARGIRNTVGFAWHPATHDLWFTDNGRDLLGDDVPDDKL
                     NRAPRAGMDFGFPYCHGGDTPDPEFGKDHPCSAFTPPVAKLGAHVAALGMRFYSGSMF
                     PAAYLDNIFIAEHGSWNRSKKVGYRVVRVITNPDGSNAHQEVFAEGWLQPNETVWGRP
                     ADVQPMPDGSLLISDDYAGAVYRVTYSAP"
     gene            complement(1479..>1659)
                     /locus_tag="VHV99_24470"
     CDS             complement(1479..>1659)
                     /locus_tag="VHV99_24470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638575.1"
                     /translation="TDAHSTPRGVAPVEAARDCIDGVADWWAPRVPRRATCTEVRVIV
                     SAGRAVCRCSDSAGA"
BASE COUNT          308 a          560 c          495 g          296 t
ORIGIN      
        1 ggcgcttctt ggagccgcca cgagccaagc ggatgatgac catactagaa tccttgaaaa
       61 ccggggttcg gaacgactga aacacgcgat tatagcggga aaccgacgcc ataacaaaca
      121 cttaccggga taaactggca gaccaggttg cgccggccat acaggcaagg ctcccggacc
      181 gcgccgaaac gcccgaatta cctgatggaa cgcccgtgaa actcctgccg ccgtctgcgt
      241 ttttcttcgc ttcgttcatc gtctttcggc ggattgccgc ttttttgttg cctgctgggc
      301 gttcgggcac agtccgcgct caggctctgc gagcgggcgc agctttgttg ctcgctgccg
      361 cggccctgcc agcgctggcc gcgttgccga tcgggcgcat caaactgccg cccggcttcc
      421 atatcgaggt cctgtcggac gcggtgccga ctgcccgagc catggcgctc tcgccaaagg
      481 gcattctcta cataggcagc ctcgatggcc acgtctatgc gctcgaactg caaaatgggc
      541 acgtgacggc acgccatgtc atcgtctcag ggctggaaac gccggccggc gtagcgtggc
      601 gcgatggcac gctgtatgtg tccgcagtgt cgaatattct acgcttcgac gcgatcgaca
      661 cccatttgag cgacccaccc aagccggcca ttgtcaccga caaactgccc accgagactc
      721 atcacggctg gaaattcatc gctttcgggc cggacggcaa gctgtacgtg ccgcaaggcg
      781 cgccctgcaa cgtctgcctg aaagatcgcg atcgcttcgg gctgattggc cgtatggacc
      841 cggacggcag ccattatgaa gtcgtggcgc gcggcattcg caacacggtc ggcttcgcgt
      901 ggcatccggc aacccatgac ctgtggttca ccgacaacgg ccgcgacttg ctgggcgatg
      961 acgtaccgga cgacaaactc aaccgcgcgc cgcgcgccgg catggacttc ggctttccgt
     1021 attgccatgg cggcgatacg ccggaccccg aattcggcaa agatcacccg tgcagcgcct
     1081 tcacgccgcc ggttgccaaa ctcggtgcgc acgtggccgc gctcggcatg cgcttttaca
     1141 gtggctcgat gtttccggcc gcctatctag acaatatttt tatcgccgaa cacggttcgt
     1201 ggaatcgcag caagaaggtc ggctatcggg tcgtgcgcgt gatcaccaac ccggacggca
     1261 gcaacgcgca tcaggaagtc tttgccgaag gctggctcca gccgaacgaa acggtgtggg
     1321 ggcggccagc cgacgtacaa ccgatgcctg acggctcact gctgatcagc gacgactacg
     1381 caggcgcggt ctatcgcgtt acctactcag cgccgtagcc ttggccttga ccgacgcgca
     1441 gccgccagct caggccgccc gtcaaatccg cgccgcgatc aggcacccgc actgtcggag
     1501 catcggcaca ctgcgcgacc cgcgctcaca atcacgcgca cttccgtgca ggtcgcacgc
     1561 cgcgggaccc gcggcgccca ccaatcagcc acgccgtcga tgcagtcgcg cgccgcctcg
     1621 actggcgcca cgccgcgtgg cgtagaatgc gcgtcggtc
//
LOCUS       DATASH010000917         5115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4108752,
            whole genome shotgun sequence.
ACCESSION   DATASH010000917 DATASH010000000
VERSION     DATASH010000917.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5115
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4108752"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..812
                     /locus_tag="VHV99_24475"
     CDS             213..812
                     /locus_tag="VHV99_24475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HEX3638576.1"
                     /translation="MPEQLWLPGLEAPPVPTDGLFFAVFPDTHTAAGISKLAQQLCGE
                     TRSKSKPLAANRLHVTLLHLGNFAGGLPPARVEAAMSAAASIRMEPFSVEFDSAHSFA
                     LKPRPGPLVLGGGEGVAGLHALHDVLLPALQNAGFGDRADSANPPYTPHVTLAYGMPW
                     IAPRPVEAVSWNVREFALVHSLLGRTRHVVLARWPLAAG"
     gene            1378..1779
                     /locus_tag="VHV99_24480"
     CDS             1378..1779
                     /locus_tag="VHV99_24480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ family domain-containing protein"
                     /protein_id="HEX3638577.1"
                     /translation="MKLLDALVEQRIAAAAARGEFDDLPGAGAPLLLEDDALVPEEVR
                     VANRILKNAGFVPPAVEQLRALRDLQAELNAVNDEASRCRLQARMLALDMALESLRGG
                     PLVLPREYCRRIAERLSERVGNLNAAEAGSQ"
     gene            1845..2516
                     /gene="tadA"
                     /locus_tag="VHV99_24485"
     CDS             1845..2516
                     /gene="tadA"
                     /locus_tag="VHV99_24485"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEX3638578.1"
                     /translation="MAGTATLPAAQPESAGSGTAVPVSDPAQTVAATAPFHTDPPPGE
                     LPAPPVSERDRRFMALAQAAAEEARAAGEVPVGAVLVRGDEVIATGFNHPIGAHDPSA
                     HAEMVALRAAAQAAGNYRLPGCELYVTLEPCLMCAGAIMHARIARVVFGARDPKTGAC
                     GSVVDAFANPQLNHHATVTGGVLETECGAALRSFFAERRRASREARAVARGEAHTEAG
                     PAEPL"
     gene            2544..3470
                     /gene="ldcA"
                     /locus_tag="VHV99_24490"
     CDS             2544..3470
                     /gene="ldcA"
                     /locus_tag="VHV99_24490"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018435542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HEX3638579.1"
                     /translation="MTVHRTIELIAPSGYPHDSEVLHRALRRLHAQGHRVNGVEATRR
                     RYQRFAGTDGERAADLNRLADPSRELPDIVLAVRGGYGAVRILHGLDYDGLQRRLTDQ
                     PVALVGHSDFTAVQLALLARAGLKTFGGPMLMSDFGAEDLSEFTMQHFWSALTQPTMT
                     VTGNAPQAQTADVSGTLWGGNLAVLASLIGTPYMPPVQGGILFVEDVNEQPFRLERML
                     YQLHLAGILAQQQALVLGDFSGGKPYDYDNGYDVHAMIEQVRSVVGIPVITGLQFGHV
                     PNMLTLPVGADAHLVAGAHGFKLTLSGYPSLG"
     gene            3789..4529
                     /locus_tag="VHV99_24495"
     CDS             3789..4529
                     /locus_tag="VHV99_24495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX3638580.1"
                     /translation="MSDTDSASSNGSKPEAIAERIRAAILEHRLAPGAKLTEAQLCEV
                     FDVKRGPIRQALAQLATDHLVDLEPNRGAFVASPSLQEVHEVFEMRRIIELAVVEKIC
                     GGHGMRRLKSIGSMIGRERKAFETRDFPAWIRLSGEFHTELAGLTGNTVLCDCLNGLV
                     ARSTLISALYESLGRSPCSFEDHEAILAALDAGDAKEAAALMARHLQTVELKMLDRPA
                     RGAADLHEVFGALNGASRVSSKGKSAPD"
     gene            4866..>5115
                     /locus_tag="VHV99_24500"
     CDS             4866..>5115
                     /locus_tag="VHV99_24500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HEX3638581.1"
                     /translation="MAQFSAASGSPAIPTYADDGAPSDPSIPAGYSERLYNEDLAPLR
                     HQTWGAYNIFAFWMSDVHSVGGYVFAGSLFALGLTSWKV"
BASE COUNT          953 a         1539 c         1632 g          991 t
ORIGIN      
        1 ccgatcttca tcgatactgt atgaatgtac agtatcgtgc gtggacggcc agtgagtgca
       61 agttcattgg gcgtgaacgg gcgcgggata ctaggggtgg gctaaacctc gccttgccct
      121 atcgagtcgt taaaattttc agatgcgtgg gcaagcccgc atcgatgcga ggagattctt
      181 cgaaatgtct gcgtcaccca ttcaggcgcg ctatgcctga gcaactctgg ctgcccggac
      241 tcgaggcgcc acctgtgccg acagacggcc tgttttttgc cgtctttcca gatacgcata
      301 cggcggcggg catctcgaag ctggcgcaac agctttgcgg ggagacgcgc tcgaaaagca
      361 agccgctcgc ggccaaccgc ttacacgtca cgctgctgca cctcgggaat ttcgcgggcg
      421 gtttaccgcc ggcacgcgtc gaagcagcaa tgagcgcggc cgcgtcgatc aggatggagc
      481 cttttagcgt cgagttcgac agcgcccaca gttttgcgct gaagccgcgg ccggggccgc
      541 tggtgttggg cggaggcgag ggtgtcgctg gacttcacgc gcttcatgac gtgctcttgc
      601 ccgcattgca aaacgctgga tttggagatc gcgcggactc ggccaatcca ccgtacacgc
      661 cgcacgtgac cctcgcttac ggcatgccct ggatcgcgcc acgtcctgtt gaagccgtca
      721 gttggaacgt gcgcgagttt gctttggtgc acagcctgct cgggcgaacc cggcatgtcg
      781 ttctggcgcg ctggccgttg gcggccggat gagggtactg gttcgtgcgg gccgttgtga
      841 actaatgcgt tgaggctttg aggcgttgag aactagaacc gcatgccggt tgcgcaaccg
      901 ccggcaaagc caccaccgct tccgcccgcg cctccgcaga acacaccgca gcctgatagt
      961 gcggccgaca tcgtaatggc cgccactgtc acgcgtaggt atcgcttgaa agcgtgcttg
     1021 cgaagttgcg cgggcagtgc gctcaggcgc ggatcgaagc gtgcggcatc agtggcataa
     1081 gcggtactag cgggaagtgc ggtgtcggag cgatggttgg tgattccgga ttgcatagcg
     1141 taaatgggct ccatgagtag tgcgtttgcg ggcggctgtt ttgtgtcgcc cgagcgaaag
     1201 atgtttgtaa gttttggcgg tgcgaagcgg cggcgccggc aaccctctac cctaagccac
     1261 gtcatgcatc cttcgaatgc gcaaaatctg aaaaccaggt agattcgtcg gtcagacagc
     1321 cagaaccgtc gcgtgctccc tgatagaatg cttgggccgc aacgtagtgg accgcagatg
     1381 aaattgcttg atgcgttagt cgaacagcgt attgccgccg cagccgcgcg cggtgagttc
     1441 gatgatttgc cgggcgcggg cgcgccgctg cttctggagg acgatgcact ggtcccggag
     1501 gaggtgcgtg tcgccaaccg gattttgaaa aatgcgggtt tcgtgccgcc agctgtcgag
     1561 cagctgcgtg ccttgcgcga cctgcaagcg gagctgaatg ccgtgaacga cgaggcttcc
     1621 cgctgccgtc tccaggcgcg catgctggcg ctcgacatgg cacttgaatc gctgcgcggc
     1681 ggcccgctgg ttctgccgcg cgaatactgc cggcgcattg ccgagcggct gtcggaacgc
     1741 gttggcaacc tcaatgcggc tgaagcgggt tcgcagtgag cgagccgctt gcccgctgtg
     1801 tcgagtcagg cccggagccg gtcgagtccg cggagtgtgt tgaattggcc gggacggcga
     1861 cgttgccggc tgcccagcct gaatccgctg gttccggcac ggcggtgccg gtgagcgatc
     1921 cggctcaaac agtcgccgcc actgcgccgt tccacaccga tcctccgcca ggcgaattgc
     1981 ccgcaccgcc tgtgtcggag cgcgatcgcc gcttcatggc gctcgcccag gccgccgccg
     2041 aagaggcccg cgcagccggc gaagtgccgg tcggcgcggt actcgtgcgc ggcgacgaag
     2101 tcatcgccac ggggttcaac catccgatcg gtgcgcacga tccgtccgcg cacgcggaaa
     2161 tggttgcgtt gcgggcagcc gcacaagccg ccgggaacta ccgtctgccg ggctgcgagc
     2221 tctacgtgac gctcgaacct tgcctgatgt gcgccggcgc gatcatgcat gcgcgcatcg
     2281 cccgcgtcgt gttcggggcg cgcgatccga agacgggcgc atgtggcagt gtcgtcgatg
     2341 cattcgcgaa tccacagttg aatcaccacg ccacggtgac gggcggcgtg ctggaaaccg
     2401 aatgcggcgc cgcgctcaga tcgttctttg ccgagcggcg tcgcgccagt cgtgaagcac
     2461 gcgcggtggc acgaggtgaa gcgcacaccg aagcggggcc ggctgagccg ctctaaaaca
     2521 acaacgtcca acaggtctcg ccaatgaccg tccatcgcac cattgaactg atcgcaccgt
     2581 ccgggtatcc gcacgactcc gaagtgctgc atcgcgcgtt gcgccgcttg catgctcaag
     2641 ggcatcgcgt caacggtgtc gaggcgacca ggcgccgata ccagcgcttc gccggcactg
     2701 acggcgagcg tgccgccgat ctgaaccggc tggccgaccc ctcgcgtgaa ttgcccgaca
     2761 tcgtgctagc cgtgcgcggt ggctacggcg cggtgcgcat cctgcacgga ctcgactacg
     2821 acgggctgca acggcggctg accgatcaac cggtcgcgct ggtgggtcat agcgatttca
     2881 ccgcggttca gctggctctg ctggcgcgcg ctggactgaa aactttcggt ggcccgatgt
     2941 tgatgagcga cttcggcgcg gaagatctga gcgaattcac catgcagcac ttctggtccg
     3001 cgctgaccca gccgaccatg acggtgacgg gcaacgcgcc gcaagcgcag actgccgacg
     3061 tgagcggcac cttgtggggc ggcaatctgg cggtgctggc atcgctgatc ggcacgccgt
     3121 acatgccccc ggtgcagggt ggcattctgt tcgtcgaaga tgtcaacgaa cagccgttcc
     3181 ggcttgagcg aatgctttat cagttgcacc tggcgggcat ccttgcgcag cagcaggcgc
     3241 tggtgctcgg cgatttctcc ggcggcaaac cctacgatta cgacaacggc tacgacgtgc
     3301 acgcgatgat cgagcaggta aggtcggtgg tcgggattcc ggttatcacc ggactgcaat
     3361 tcggccacgt tcctaacatg ctgacgctgc cggtcggcgc ggacgcgcac ctcgtcgccg
     3421 gcgcgcatgg gttcaaattg acgttatcgg gctatccgtc tctcggctga cattccgtag
     3481 gggcagggaa gcagcgggca tttgcccgct ttcttttcga gtttttaacg caactaacgg
     3541 tctagtattt gtctttaccg ccaacagctg tgtccgtgaa ggtacctcta ttttgttgac
     3601 aaaaaccgct gccaatagac ggcttcgtca cgccttaatt aaactggtac ggaagccacg
     3661 caaggctcat gcactgcgcc aatgggtccc caaccaatgg cactatcatg attgtcaaaa
     3721 ttaaaagcaa aaattgttga caatttttat gtccatccta tgatgaccaa cataccgaga
     3781 caaacatcat gtccgacaca gattccgcca gctcgaatgg ttcgaaaccc gaagcgatcg
     3841 ccgagcgcat ccgcgccgcg atcctcgagc atcggctcgc acctggtgcc aagctgacag
     3901 aagcacagtt atgcgaagta tttgacgtga agcgaggacc gatccgccag gcgctcgcgc
     3961 aactggcgac cgaccacctc gtcgacctcg agccgaatcg tggtgcgttc gttgccagcc
     4021 cgtcgctgca ggaagtgcat gaagtgttcg aaatgcgccg gatcatcgaa ctggccgtgg
     4081 ttgaaaagat ttgcggcggt catgggatgc gccgtttgaa gagcatcggc agcatgatcg
     4141 gccgtgaacg caaagcgttc gaaacacgtg attttccggc gtggattcgt ctgtcgggcg
     4201 agtttcatac cgagttggcc gggctcaccg gcaacaccgt gctgtgcgat tgcctgaacg
     4261 gactggtggc gcgctccacg ctgatttcgg cactgtacga gtcgctcgga cgcagccctt
     4321 gctcgttcga agaccacgaa gcgattctcg ccgcgctcga cgccggcgat gcaaaagagg
     4381 ccgcggcact gatggcgcgc catttgcaaa ccgtcgagtt gaagatgctg gaccgccccg
     4441 cacgtggcgc tgccgatctg catgaagtgt ttggcgcact taacggtgcg tcccgggttt
     4501 catccaaagg caagtccgcg ccagactgag gcggcggccg gtgcgcgctc cttgctggtg
     4561 cgcaccgcac tgaattgaac tgaagcgaat caagcgttgc aaggcggctc ggcgtgagcg
     4621 ctgccggctt atgcacgctt ggacgtcatc gaatggcagc cttgaaagta gtcacgcagc
     4681 gccgggattc gcggctaccg gcgctgcgat aaccacaagt acatcaagta cggagacatc
     4741 cgcggcacgg tcaatcagct agcttgaacc ttctgtttcc cgatcaggga agaaggacgc
     4801 gactggcatg gccgatgcaa cgtccttcga ttcgcaatcg acggtcccta tcccaaggag
     4861 gagtcatggc tcagttcagt gcagcgtccg gcagtcccgc aattcccacc tatgcagacg
     4921 acggcgcgcc cagcgatccg tcgatacccg caggctacag cgagaggctg tacaacgaag
     4981 acctggcgcc gctgcgccat caaacctggg gtgcctataa catcttcgca ttctggatgt
     5041 cggacgtgca cagcgtcggc ggctatgtgt tcgcgggcag tctgttcgcg ctcgggctga
     5101 cgagctggaa ggtat
//
LOCUS       DATASH010000918         7012 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6089476,
            whole genome shotgun sequence.
ACCESSION   DATASH010000918 DATASH010000000
VERSION     DATASH010000918.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7012
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6089476"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..704
                     /locus_tag="VHV99_24505"
     CDS             18..704
                     /locus_tag="VHV99_24505"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX3638582.1"
                     /translation="MLTALAFDAYATSSAARQVLVEAYGDSTTLGITCSNGHCGPQAQ
                     NAVAYLQDELQARYGERVRVTNYGVGGTMASQLRDGTGNRRAGPTAGLPWQERLAASS
                     AKIVLINYGINEVMQNQTPEQFYAAETTLVKTARALGKEPVLQTSNPMPDSRLNTRLA
                     AMAAMTRRVATEQQVPLVDQFAYISDLPDWKTLMSDGAHPKPGLYRLKAEQDFQVVDP
                     LVRRLLDGTL"
     gene            759..1802
                     /gene="gmd"
                     /locus_tag="VHV99_24510"
     CDS             759..1802
                     /gene="gmd"
                     /locus_tag="VHV99_24510"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006028634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX3638583.1"
                     /translation="MSQPRKAIITGVSGQDGAYLTKLLLDKGYQVTGTYRRTSSVNFW
                     RIRELGVLDHPNLRLVEHDLTDLGSTLRLLEGAQADELYNLAAQSFVGVSFDQPVTTA
                     EVTGIGALNLLEGIRILNPKTRYYQASTSEMFGLVQAVPQREDTPFYPRSPYGVAKLF
                     AHWSTINYRESYNLFASSGILFNHESPLRGREFVTRKITDTVAKIKLGKQDVLELGNL
                     SAKRDWGFALEYVEGMWRMLQADEPDTFVLATGRTETVRDFVRMAFAAAGYQIEWSGK
                     EERETGIDVATGKTLVRVNPKFYRPAEVDLLIGCADKAHEKLGWKPETTLEQLCHMMV
                     HADLGRNEHHETF"
     gene            complement(1899..3065)
                     /locus_tag="VHV99_24515"
     CDS             complement(1899..3065)
                     /locus_tag="VHV99_24515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX3638584.1"
                     /translation="MTTSSIKSLQIGMHWFPERAGGLDRMYYSLVGALPGAGVAVRGV
                     VAGSPKVAADTNGAINGFGSAAQSLPLRLMAARRALRQEISTSRPDVISSHFALYTFP
                     GLDVTRGIPQVSHFQGPWADESHVEGADSLGQRAKRYLEQSVYARSSRLIVLSEAFGK
                     ILTSRYRISPERVRVVPGCVDVEQFNLPITPAEARLKLQLPQDRPIVLAVRRLVRRMG
                     LEDLIDAVKLLKRTEPDVLLLIAGKGRLEGELQARITEAGLEDNVKLLGFVPDQHLAA
                     LYRAANISVVPTVALEGFGLITVESLASGTPVLVTPVGGLPEAVAGLSPNLVLPETGA
                     KAIAEGLAGALNGSLKLPDAEACRRYAREHFDNAVIAKRVASVYSEAIGAGGVH"
     gene            complement(3266..4495)
                     /locus_tag="VHV99_24520"
     CDS             complement(3266..4495)
                     /locus_tag="VHV99_24520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX3638585.1"
                     /translation="MRVAIVTHVVRHNDGQGRVNHEIARAALAENIAVTLVASHVAPD
                     LLAHPNVRWVPMKIGKWWPTNLLRQQVFAFRSAMWLRAHRREYDVLHVNGFITWMPAD
                     VNTSHFVHSGWFGSKYYPFGLTKGVWSAYQSVYTRCNAVLERWAYRRSKVITAVSQKV
                     ADEIRAIGLTPHNRVDVIYNGVDTQGFAAATGDRAKFGLPADAFLLLFVGDLRTPRKN
                     LGTVLQALRHLPDHVQIAVAGFLPGSPYPEEAKALGIAHRVHFLGLVKDMPVLMHSVD
                     AFVFPSRYEAMSLSLLEAMAAGLPVVTARTAGGAEIITPECGIVLDDPDDPKALAGAV
                     ARLADNGEARRAMGVAANELATGFGWARMAAQYIALYRQLAGQQKDRRRNEAEASVVA
                     KTDALTLNTLAGQKGAE"
     gene            complement(4492..5955)
                     /locus_tag="VHV99_24525"
     CDS             complement(4492..5955)
                     /locus_tag="VHV99_24525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEX3638586.1"
                     /translation="MSTITRAGGDNAGRVLSGARNEWLPEALLWLITAMLIIAHQGTV
                     LTLGFPVLAILTGLWLYFKSPARYIGFMWWLWFLSPEVRRLADWSKGSFTPTSLIQVA
                     PLAVTMISAFSLLRYYKLLSQRRGLPVLLILMGLAYAFMIGVMSSGPLAATYDLANWL
                     YPILIGFHIMAHTRQYPKYRDTIVNTFIWGMLAMGGYGLIQFFIMPQWDALWMLGSQM
                     NSQGDPVPMGVRVFSTMNSSGPFAFAMMGAMVYVMAATHRVRWIAAALGFFSFALSLV
                     RSTWGGWVIALLILLVKSNNKVRVRIVASAILLAGLCVPLLAVGPVAERMQARLTTIV
                     NLNDDQSYAARNEFYATFAKTAFTDVSGEGMGATGTSTKLSNDGGQLGQYGNFDSGVM
                     NIPFVLGWPGTLLYMSGIVWLVVRAVLASFKLRNDKFVSACLSLSLATFAMLVFTNSL
                     VGTGGLLLFMSVFSILSAVHYEKMNRGRTLFFHGGTD"
     gene            complement(5959..>7012)
                     /locus_tag="VHV99_24530"
     CDS             complement(5959..>7012)
                     /locus_tag="VHV99_24530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3638587.1"
                     /translation="IHTWRNRAEMLRSDVIWTHTEQEWLSAALMLLLSGRKAGAEAAP
                     LLLAQSVWLLDKWPSYGILRRWLYRKLMSRADQLTTLASENADLCQRYFNRDATPLLY
                     GLNTQDFPVKAPTGWTPHTPIRIAAIGNDRDRDWETLIKAFGNDARYTVKLATRRRIP
                     ASLRAPNVEIALFSGIKKQHELYDWADVIVVPLRPNSHASGITVMLEAAAVGKPMVVT
                     NVGALQDYFPEGEAAYIPPFNPQALRETVDQLAASPMDALRQAQAAAAGLLSRDLTTQ
                     NYAMQHVRITQEMLLSRAASRGRVAPGAADADATAPRVAPAAAPVNQPVRVRAGSQGQ
                     AQGQRQRPASQSRGGL"
BASE COUNT         1324 a         2312 c         2185 g         1191 t
ORIGIN      
        1 tgtcggctgc ggcggcggtg ctgactgcac tggcattcga cgcatatgcg acgagctccg
       61 ccgcgaggca ggtgctggtg gaggcctacg gcgattcgac cacgctcggt atcacttgca
      121 gcaacggcca ctgcggaccc caggcgcaaa acgccgtggc gtatctgcag gacgaattgc
      181 aagccaggta cggtgagcgc gtgcgcgtca cgaactacgg cgtgggcggc acgatggcca
      241 gccagctgcg cgacggcacc ggcaatcgcc gcgccggtcc gacagccggc ctaccgtggc
      301 aggaacggct cgccgcttcg tccgcgaaga tcgtgttgat caactacggc atcaacgaag
      361 tgatgcagaa ccagacgccc gagcagtttt acgcggccga aacgacgctt gtaaaaaccg
      421 cccgcgcgct cggcaaggag ccggttctgc agacctcgaa tccaatgccg gatagccggc
      481 tcaatacgcg gctcgccgcg atggccgcga tgacgcgccg cgtggccacc gaacagcagg
      541 tgccgctcgt cgatcagttt gcctatatca gcgacctgcc ggactggaag accttgatgt
      601 ccgacggcgc gcatccaaag ccgggcttgt accggctcaa ggcggaacag gattttcagg
      661 tcgtcgaccc gctggtgcgg cgactgcttg acggcacgct ttgaagcgct gtttcaagtg
      721 cacttctctt tctcttttac caatctcgaa ggcaagccat gagccaacca cgcaaagcga
      781 tcatcaccgg cgtctccgga caggacggcg cctatctgac caaactgctg ctcgacaagg
      841 gctatcaggt gaccggcact taccggcgca cgagttcggt caacttctgg cgcattcgtg
      901 agctcggcgt gctggatcat ccgaatctgc gtctggtcga acacgatctg accgacctgg
      961 gctccacgct gcgtctgctc gaaggcgcgc aggccgacga gctgtacaac ctcgccgcgc
     1021 agagcttcgt gggcgtttcg ttcgaccagc ccgtgacgac cgctgaagtg accggcatcg
     1081 gcgcgctgaa tctgctcgaa ggcattcgca ttctgaatcc gaagacgcgc tactaccagg
     1141 cgtcgacctc ggaaatgttc ggtctcgtgc aggcggtgcc gcaacgcgag gacacgccgt
     1201 tctatccgcg cagcccgtat ggcgtggcca agctgttcgc gcactggtcg accatcaact
     1261 atcgcgagtc gtacaacctg tttgccagca gcgggattct gttcaatcac gaatcgccgc
     1321 tacgtggccg cgaattcgtc acacgcaaga tcaccgacac ggtcgcgaag atcaagctcg
     1381 gcaaacagga cgtgctggaa ctcggcaacc tgagcgccaa gcgcgactgg ggcttcgcgc
     1441 tcgaatacgt ggaaggcatg tggcgcatgc tgcaagccga cgaacccgac accttcgtgc
     1501 tcgccaccgg ccgcaccgag acggtccgcg acttcgtgcg catggcgttc gccgccgcgg
     1561 gttatcagat cgaatggtcg ggcaaggagg agcgtgagac cggtatcgac gtcgccaccg
     1621 gcaagaccct ggtgcgcgtg aatccgaagt tctaccgccc ggcggaagtg gacctgctga
     1681 tcggctgcgc ggataaggcc catgagaagc ttggctggaa gccggagact acgctcgaac
     1741 aactgtgcca catgatggtt catgcggatc ttgggcgcaa cgagcatcac gagacattct
     1801 gaaccggtgt gacgacgagc ggccgcgtgc ggtcgcccga tgcaaaaaag gcccgatcgt
     1861 catgattcag gccttttttc tactacgtca tgaaaacttc aatgcacccc gcccgccccg
     1921 atcgcctcgc tatacaccga ggccacccgc ttcgcgatca ccgcgttatc gaagtgttca
     1981 cgtgcatagc ggcggcaagc ctcggcatcc ggcaacttta gcgagccatt caacgcgccc
     2041 gccagacctt ccgcaatcgc cttcgccccc gtctccggca ataccagatt cggcgacaac
     2101 cccgccactg cttccggcaa gccgcccacc ggcgtcacca gcacgggcgt gccggaagcc
     2161 agcgattcga cggtaatcag cccgaagcct tcaagcgcca ccgtcggcac cacgctgatg
     2221 ttcgccgcac ggtacaaggc cgccagatgc tgatcgggca cgaagcccag cagcttcaca
     2281 ttgtcctcga gaccggcttc ggtgattcgc gcctgcagtt cgccttcgag ccgccccttg
     2341 cccgcgatca gcagcagcac atcgggctcg gtgcgcttga gcagcttcac cgcatcgatc
     2401 agatcctcga gccccatacg gcgcaccaga cgccgcaccg ccagcacgat cggccggtct
     2461 tgcggcaatt gcagcttcag gcgcgcctcg gccggcgtga tcggcagatt gaactgctcg
     2521 acatccacgc agcccggcac gacgcgcacg cgctccggtg aaatgcgata acgcgaagtc
     2581 agaatcttgc cgaaggcctc cgacaacacg atcagccgcg acgagcgcgc atacaccgat
     2641 tgctccagat agcgcttggc ccgctggccg agcgagtcgg ccccttccac atggctctcg
     2701 tcggcccacg gtccctgaaa atgcgagacc tgtggaatgc cgcgcgtcac gtcgagtccc
     2761 ggaaacgtgt acagcgcgaa gtgcgacgaa atcacgtcgg gacgcgaggt gctgatctcc
     2821 tggcgcagcg cgcggcgtgc cgccatcagg cgcagcggca gcgattgcgc ggccgagccg
     2881 aagccgttga tcgcgccgtt ggtgtccgcg gcgactttcg gcgagccggc caccacgccg
     2941 cgcaccgcga cgcccgcgcc cggcaatgcg ccgaccagcg agtaatacat gcggtcgagg
     3001 ccgcccgcgc gctcgggaaa ccagtgcatc ccgatctgca gcgatttgat tgagctcgtc
     3061 gtcatgattg ttgcctctgt gcgttctgaa agttgtgatg gaatgcggca cgtttcggcc
     3121 agccggttcg tcaacgggtc cgcgcgttgg ctcacgttcg ggtcccgccg gcgctgcgga
     3181 tcaaggcgtc cgttcgggct ccgtcggcgc tccatatcaa ggctcacgtt cgggtcccgc
     3241 cggcgctgcg gatcaaggcg tccgttcatt ccgcgccctt ctgcccggcc agcgtgttca
     3301 gcgtcagcgc atcggtcttg gcgaccaccg aagcttccgc ctcattgcgt cggcggtcct
     3361 tctgctgccc cgccagttgc cggtacagcg cgatgtattg cgcggccatg cgggcccagc
     3421 cgaagccggt cgcaagttcg ttcgcggcga cgcccatcgc gcggcgcgcc tcgccgttgt
     3481 cagcgaggcg cgcgacggcg ccggccaatg ccttgggatc gtccgggtcg tcgagcacga
     3541 tgccgcattc cggcgtgatg atttcggcgc cgcccgcggt gcgcgcggtg acgaccggca
     3601 agcccgcggc catcgcttcg agcagggaca ggctcatcgc ttcgtaacgc gacgggaaca
     3661 cgaacgcgtc gacggaatgc atcagcaccg gcatgtcctt caccaggccg agaaaatgca
     3721 cacgatgcgc aatcccgagc gctttcgctt cttccggata cgggctgccc ggcaggaatc
     3781 cggccaccgc gatctgcacg tggtcgggca aatgcctgag tgcttgcaac accgtgccga
     3841 ggtttttgcg cggcgtgcgc aagtcgccga cgaacaacag caggaaggcg tccgcaggca
     3901 aaccgaattt cgcgcggtcg cccgttgcag ccgcgaagcc ttgcgtatcg acaccgttgt
     3961 aaatcacgtc gacgcggtta tgaggcgtca agccgatcgc gcgaatttca tcggcgacct
     4021 tttgcgacac cgccgtgatc accttcgagc ggcggtaagc ccagcgttcg agcaccgcgt
     4081 tgcagcgcgt ataaaccgac tgatacgcgg accacacgcc cttggtgagt ccgaacgggt
     4141 agtacttgct gccgaaccag ccgctatgca cgaaatgcga ggtgttgacg tcggcgggca
     4201 tccacgtgat gaagccgttc acgtgcagca cgtcgtattc ccggcgatgg gcgcgcagcc
     4261 acatcgcgct cctgaacgcg aagacctgct ggcgcagcag attcgtcggc caccatttgc
     4321 cgattttcat cggcacccag cggacgttcg gatgcgcgag caaatccggc gcgacgtgcg
     4381 acgcgaccag cgtgaccgcg atattttcag cgagggcggc gcgcgcaatc tcgtgattga
     4441 cgcgtccctg gccgtcgtta tggcgtacga cgtgcgtaac aatggcgact ctcaatcagt
     4501 gcctccgtgg aaaaatagcg tgcgtccgcg gttcatcttt tcgtagtgca ccgcggaaag
     4561 gatcgaaaaa acactcatga aaagcagcag gccgccggtg ccgaccagcg aattggtgaa
     4621 caccagcatc gcgaaagtcg cgagactgag gctcaggcag gcggacacga acttgtcgtt
     4681 gcgcagcttg aacgacgcga gcaccgcgcg caccaccagc cacacgatgc cggacatgta
     4741 cagcaaagtg ccgggccagc cgagcacgaa cgggatgttc atcacgccgc tgtcgaagtt
     4801 gccgtactgg ccgagctggc cgccgtcgtt cgaaagcttg gtggaggtgc cggttgcgcc
     4861 catgccttcg ccggagacat cggtaaaggc tgttttcgca aaggtcgcgt agaactcgtt
     4921 acgcgcggcg tagctctggt cgtcgttgag gttgacgatg gtggtgaggc gcgcctgcat
     4981 ccgctcggcc accgggccga ccgcgagcag cggcacgcac aggccggcca gcaaaatcgc
     5041 actcgcgacg atccgcaccc gcaccttgtt gttcgacttg accagcagga tcaacagcgc
     5101 gatgacccag ccgccccagg tcgaacgcac gaggctcagc gcgaacgaga agaagccgag
     5161 cgccgccgcg atccagcgca cgcggtgcgt cgccgccatc acgtagacca tcgcgcccat
     5221 catcgcgaac gcgaacgggc ccgacgagtt catcgtgctg aacacgcgca cgcccatcgg
     5281 cacgggatcg ccttgcgaat tcatctgcga gccgagcatc cacagcgcgt cccactgcgg
     5341 catgatgaag aactggatca ggccgtagcc gcccatcgcc agcatgcccc agatgaaggt
     5401 attgacgatg gtgtcgcggt atttcggata ctgacgcgtg tgcgccatga tgtgaaagcc
     5461 gatcaggatc ggatagagcc agttcgccag gtcataggtc gcggcgagcg gcccgctcga
     5521 catcacgccg atcatgaacg cgtaggcaag ccccatcagg atcagcagca ccggcaggcc
     5581 gcgccgttgc gacagcagct tgtagtaccg caataaggag aacgcactga tcatcgtcac
     5641 cgcgagcggt gcgacctgga tcaggctggt cggcgtgaac gagcctttgg accagtcggc
     5701 gagacgccgc acttccgggc tcaggaacca cagccaccac atgaagccga tgtagcgcgc
     5761 cgggcttttg aagtaaagcc acaggcccgt caggatcgcg agcaccggga agccgagcgt
     5821 gagcaccgtg ccctgatgcg cgatgatcag catcgcggtg atcagccaca gcagcgcttc
     5881 gggcagccat tcattgcgag cgccggacag cacccgcccc gcgttgtcgc cgccggctcg
     5941 cgtgatggtc gacatcgttc agaggcctcc gcgtgattgg cttgcagggc gttggcgttg
     6001 accttgcgct tgcccttggc tccccgcgcg aactcgcacc ggctggttca cgggggcggc
     6061 ggccggcgcg acacgcggcg cagttgcgtc ggcgtcggct gcgccaggcg ccacgcgtcc
     6121 acgcgacgcg gcgcgcgaca gcaacatctc ctgcgtgatc ctcacatgct gcatcgcata
     6181 gttctgcgtg gtcagatcgc gcgacagcag accggcagcc gccgcctgcg cctggcgcaa
     6241 cgcatccatc ggcgaggccg cgagctgatc caccgtctca cgcaacgcct gcgggttgaa
     6301 cggcgggata taagccgcct cgccctccgg aaaataatcc tgcagcgcgc cgacgttcgt
     6361 gaccaccatc ggcttgccga ccgccgcggc ttccagcatc acggtgatgc ccgaggcgtg
     6421 ggaattcggc cgtaagggca cgacgatcac gtcggcccag tcgtacagtt cgtgctgctt
     6481 tttgatgccc gagaacaacg cgatctcgac attcggcgcg cgcagcgagg cgggaatccg
     6541 ccggcgtgtc gcaagcttca ccgtatagcg cgcatcgttg ccgaacgcct tgatgagcgt
     6601 ctcccagtcg cggtcgcgat cgttgccgat cgcggcgata cggatcggcg tgtgcggtgt
     6661 ccacccggtg ggcgccttca cgggaaagtc ctgcgtattc aggccgtaca acagaggcgt
     6721 cgcgtcgcga ttgaagtagc gctggcacaa gtctgcgttt tcgcttgcaa gcgtagtgag
     6781 ttgatcggcg cggctcatca gcttgcgata cagccatctg cgcagaatcc cataggaagg
     6841 ccatttatcg agcagccaca cgctttgcgc gagcagtaga ggcgctgcct cagcccccgc
     6901 cttgcggccg ctcagcagca gcatcagcgc ggcggacagc cattcctgct cggtatgcgt
     6961 ccagatcacg tccgagcgca gcatctcggc gcggttgcgc catgtatgga tg
//
LOCUS       DATASH010000919        11981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3914034,
            whole genome shotgun sequence.
ACCESSION   DATASH010000919 DATASH010000000
VERSION     DATASH010000919.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 11981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11981
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3914034"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..907)
                     /gene="ugpA"
                     /locus_tag="VHV99_24535"
     CDS             complement(23..907)
                     /gene="ugpA"
                     /locus_tag="VHV99_24535"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011350698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HEX3638588.1"
                     /translation="MEKRSSFGTSVLPYLLVAPQLLITLLFFLWPAGEALWQSTQSQD
                     AFGTSSEFVGLANFKQLFADPLYLSSFNTTLIFCALVTVSGLVISLLLAVCADRVTRG
                     AKTYQTLLIWPYAVAPAIAAVLWSFLFNPSIGLVTYALAKYGIVWNHALNAGQAMFLV
                     VLASVWKQVSYNFLFFYAGLQAIPRSLIEAAAIDGAGPVRRFFGIALPLLSPTSFFLL
                     VINITYAFFDTFPVIDAATGGGPAQATRTLIYKIFAEGFQGLDIGSSGAQSVILMVIV
                     VALTVVQFRFIERRVQYS"
     gene            complement(979..2304)
                     /gene="ugpB"
                     /locus_tag="VHV99_24540"
     CDS             complement(979..2304)
                     /gene="ugpB"
                     /locus_tag="VHV99_24540"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HEX3638589.1"
                     /translation="MSCKPLFRSLSVAAVLSFGISQAAHAATEIQFWHGMEAALGERL
                     NDIANAFNASQSDYKIVPVFKGTYDQTLAAGIAAYRSGNAPAILQVYEVGTATMMQAK
                     KAVIPVSEVFKQAGVPLDEKAFVPTIASYYSDAKSGELISMPFNSSTPVLYYNKDAFK
                     KAGLDPNQPPKTWAELQVDAQKLKASGMACGYSSGWQSWIQLENYSAWHGAPFASKNN
                     GFDGADAQLEFNKPLQVAHIQFLQNMEKEGTFTYVGRKDEPVSKFYSGDCGIITNSSG
                     SLATIKKYAKFNFGTGMMPYDASVKGAPQNAIIGGASLWVLSGKDPAVYKGVAKFLAY
                     LSSAPVAAKWHQDTGYLPVTTAAYQLTQQQGFYEKNPGSDTAIKQMLNKPPLPFTKGL
                     RLGNMPQIRTVIDEELEQVWAGKKTPQQALDSSVSRGDELLRRFEKAGN"
     gene            2760..3665
                     /locus_tag="VHV99_24545"
     CDS             2760..3665
                     /locus_tag="VHV99_24545"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015600414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX3638590.1"
                     /translation="MGCALTSEMADARVTPVTSLPKIIAHRGGTGDAPENTLEAIRQS
                     IAHHADEMWLTVQLSKDGVPVLYRPADLSALTNAKGPVSEFTAAELARVNAGWNFKSG
                     DIYPYRNHPIGIPTLREALRTIPLATPIILDMKALPAGPQAQAVAQVLNEESAWSRVT
                     IYSTEADYQQSFAAYSQARLFESRDATRGRLVRVLLNQGCVDAPAEHTAAAFELHRTM
                     TVVEKFTLGEGRSEVQATLWTPTTVACFRQKPDVRIVAIAVNNAADYRTAACLGIDAV
                     LADSPQRMAAIKAGMAMPLRCEAQK"
     gene            complement(3870..4964)
                     /gene="ychF"
                     /locus_tag="VHV99_24550"
     CDS             complement(3870..4964)
                     /gene="ychF"
                     /locus_tag="VHV99_24550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEX3638591.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTKAGIAAENYPFCTIEPNVGIV
                     EVPDARLKALAEIVKPERILPAVVEFVDIAGLVAGASKGEGLGNQFLANIRETDAITH
                     VVRCFEDENVIHVANKIDPLSDIEVINTELALADLATVEKALTRYSKAAKSGNDKEAA
                     RNAAVLEKVRAQLDQAKPVRALDLSDEEKATLKPFCLITAKPTMYVANVKDDGFQNNP
                     HLDAVTKFAAAEGAPVVAVCAAIEAEIADLEEADMEVFLADMGMEEPGLNRVIRAGFK
                     LLGLQTYFTAGVKEVRAWTIHVGDTAPQAAGAIHTDFERGFIRAQTIAFNDFITYKGE
                     QGAKEAGKMRAEGKEYVVHDGDVMNFLFNV"
     gene            5317..5622
                     /locus_tag="VHV99_24555"
     CDS             5317..5622
                     /locus_tag="VHV99_24555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638592.1"
                     /translation="MVAWLSPNYEEAQAATDRQTVDCMVKQLTLEDKRNIAQFADTND
                     FDSLWRVYDRVFPDCAVRGDQRDRKSELVTSAWRVLSTDRDFMLLRETHAAHAALGR"
     gene            5687..6859
                     /locus_tag="VHV99_24560"
     CDS             5687..6859
                     /locus_tag="VHV99_24560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006029814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiH/UbiF family hydroxylase"
                     /protein_id="HEX3638593.1"
                     /translation="MNAHHQTFDAAVIGGGLVGKTAALALTQGGLRVALLAQPCAALP
                     AGAAFDSRVYALSSSSQALLERLRVWQALDLSKLGPVYDMRVYGDAHAELHFSAFQAS
                     VPQLAWIVESSVIERALDAALRFQPNLTWIDARAQALDFTADGASVGLANGNVLEADL
                     VVGADGAHSLVRAQIGSKMNRRDYKQTGVVANFKAEKPHGETAYQWFKDGEIIALLPM
                     PDGHVSLVWSARTEHAEQLIALDPAQLAAEVERVTGGQFGALECMTPAQGFPLALQTV
                     DKLVAPRVALVGDAAHLIHPLAGQGMNLGLRDVAALAETVARKEAFRDLGDMVLLRRY
                     ERARREDIRALMVATDGLQKLFSAPGPFAKVLRNTGMAFVGAQPFIKRWLVSAALG"
     gene            6994..7719
                     /locus_tag="VHV99_24565"
     CDS             6994..7719
                     /locus_tag="VHV99_24565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HEX3638594.1"
                     /translation="MKKSFRIAAVVLAIAAVAIGCSAQADQTTDKLKATLQTRLNDVT
                     IKSVSKSPIAGLYEVNLGTQIIYSDANGDYLMLGDMVDAKTRKNLTEARLSETNRIDF
                     ASLPFADAVKVVKGNGARKIAVFSDPNCPYCKQLETSLKSIDNVTVYTFLYPVLSPDS
                     TVKSKSIWCSPDRAKAWESWMQDHQSPTAAGTCNTAAIDKNLALGRAMGVDGTPTVFL
                     ADGRRLPGAVPADRLDREMSAVH"
     gene            7815..9605
                     /locus_tag="VHV99_24570"
     CDS             7815..9605
                     /locus_tag="VHV99_24570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX3638595.1"
                     /translation="MKPIRYTIVPKQPAAHLFEVTVTVADPDPAGQRFMLPVWIPGSY
                     MVREFARNIVTLRAVNEAGRKVRVEKTDKHTWQAAPVKGALTLRYEVYAWDLSVRAAH
                     LDDTTGFFNGTSVFLSPLGHEEAQCVVDIQKPEGAAYRNWRVATALPEARGTKRYGFG
                     EYRAQNYDELVDHPVTLGEFALASFKAHGVPHDIVIAGRVIGLDMARLSADLKRICEA
                     QIALFEPKSKKAPVDRYVFMTQAVTDGYGGLEHRASTALICNRTDLPVEGRDTPTEGY
                     RTYLGLCSHEYFHTWNVKRIKPAVFAPYDLSVENYTPLLWLFEGFTSYYDDLILVRTG
                     LISQDDYLGMLGKVIGGVLRGSGRLKQTVAESSFDAWVKYYRQDENAPNAIVSYYTKG
                     SLVALAFDLTIRAQTSNRKSLDDVMRLLWQRFGRDFYRGKPVGVEASEIEALFAEATG
                     AQLGELFAEAVHSTHDLPLDTLLAPFGVTLAPELDKNGKPSLGARMRSGADCTLAAVH
                     EGSAAQKAGLSAGDVLVALDGLRVTGSNLDALLSRYLPGAKVEVHAFRRDELRTAQVK
                     LDGSEVARYKLTVSDKQPAARKARERWLAS"
     gene            9759..10355
                     /locus_tag="VHV99_24575"
     CDS             9759..10355
                     /locus_tag="VHV99_24575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012402075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX3638596.1"
                     /translation="MTTILQINSAARSQGANSTLLVNELTAKLQQSNPGAQVVVRNLQ
                     AEPLPHLDDAVLGAFFTPADQRTPEQVTIAARSEALIAELQAADIVVIGAPMYNFGIS
                     SQLKTYFDFIARAGITFQYTANGPVGLVEGKKVFVVSARGGKYLGTPNDSQTPYLKSF
                     LGFLGMTDVSFIYAEGLNMGPEAASAALAGAREAIAAA"
     gene            complement(10576..11415)
                     /locus_tag="VHV99_24580"
     CDS             complement(10576..11415)
                     /locus_tag="VHV99_24580"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX3638597.1"
                     /translation="MTSASRTRSNSSQASLFGDASATPSSATAQQQTSVAADSPPPTL
                     EAQFAALPPAWRAHLKPFIESDAYAPLCRFVDGERAAGKTVYPTDVFRALRLTSPDEV
                     KVVILGQDPYHGEDRGTPQAHGLAFSVAPNVRTPPSLRNIFKEIAASLGHEPPRHGCL
                     DTWAKQGVLLLNTVLTVERDSAASHAKRGWEKCTDTLIHELAVRHDGLVFMLWCAHAQ
                     AKRALLGGKSHYVLEAPHPSPLSAHRGFLGCGHFASANEYLVKHGRTPIDWRLPDAAQ
                     MLA"
     gene            complement(11629..>11981)
                     /locus_tag="VHV99_24585"
     CDS             complement(11629..>11981)
                     /locus_tag="VHV99_24585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEX3638598.1"
                     /translation="LEIEALLRQCDEPAAAQALRRAAPELIELFRTNFTRTLWNVALD
                     GSEVEAAIDQGDVIAEVNGEMRRAPISEVELELKSGDEAVLDTLAKELGKQVAGLAPD
                     NISKAQRGYQLRAD"
BASE COUNT         2225 a         3649 c         3836 g         2271 t
ORIGIN      
        1 tcgaaaccgc gtcggttctc gatcatgagt attgaaccct gcgttcgatg aagcggaatt
       61 gcacgaccgt caacgcgacg acgatcacca tcaggatgac ggactgcgca cccgagctgc
      121 cgatgtcgag tccctggaaa ccttcggcga agatcttgta gatcagcgtg cgggtggctt
      181 gcgcggggcc gccgccggtg gcggcgtcga tcacggggaa ggtgtcgaaa aacgcgtagg
      241 tgatgttgat caccagcagg aagaagctgg tcggcgagag cagcggcagt gcaatgccga
      301 agaaacgccg cacgggaccg gcgccgtcga tggctgctgc ttcgatcagc gagcgcggaa
      361 tcgcctgcaa gccggcatag aagaacagaa agttatagct gacctgtttc cacacggatg
      421 ccagcacgac cagaaacatc gcctggcccg cattcagcgc gtgattccac acgatgccgt
      481 acttggcgag cgcataggtg acgaggccga tgctcgggtt gaacaggaac gaccacaaca
      541 cggcggcgat ggccggcgcg accgcatacg gccagatcag gagcgtctgg taggtcttcg
      601 cgccgcgcgt gacgcgatcc gcgcagaccg ccagcagcaa ggagatgacc agtcccgaga
      661 ccgtgaccag cgcgcagaaa atcagcgtcg tattgaacga cgacagataa agcggatcgg
      721 cgaataactg cttgaaattg gcgagtccga caaactcgct cgacgtgccg aacgcgtcct
      781 ggctctgcgt ggattgccac agcgcttcgc ccgccggcca gagaaagaac agcagcgtga
      841 tcaggagttg cggcgcaacc agcaggtagg gcagcacgct ggtgccgaag ctagagcgct
      901 tttccatcga ggattcccaa ggttctactg caacatcggg tttcggtgcg cacgcaccga
      961 aaccccgccg gcgggtactt agttacccgc tttctcgaag cggcgcagca gctcgtcgcc
     1021 acgcgacacg gatgaatcga gcgcctgttg cggcgtcttc ttccccgccc agacctgttc
     1081 gagttcttcg tcgataacgg tgcggatctg cggcatgttg cccagacgca aaccctttgt
     1141 gaacggcagc ggcggcttgt tgagcatctg tttgatcgcc gtgtcgctgc ccggattctt
     1201 ctcgtagaag ccttgctgct gcgtcagttg atacgcggcg gtcgtgaccg gcagatagcc
     1261 ggtgtcctga tgccacttcg ccgcaaccgg tgcggacgac agatacgcga ggaacttcgc
     1321 cacgcccttg tagacggccg ggtctttgcc cgacagcacc cacagactgg cgccgccgat
     1381 gatcgcgttc tgcggcgcac ccttcacgct cgcgtcgtac ggcatcatgc cggtgccgaa
     1441 gttgaacttg gcgtacttct tgatcgtggc gagcgacccc gacgagttcg tgatgatccc
     1501 gcagtcgccg ctatagaact tcgacacggg ttcgtccttg cggccgacgt acgtgaaggt
     1561 gccttccttc tccatgttct gcaggaactg gatgtgcgcg acctgcagcg gcttgttgaa
     1621 ttcgagctgg gcgtcggcgc cgtcgaatcc gttgttcttc gacgcgaacg gcgcgccatg
     1681 ccaggcgctg taattttcaa gctgaatcca gctctgccag cccgacgaat agccgcacgc
     1741 catgcccgag gccttcagct tctgcgcatc cacctgcaat tcagcccagg ttttcggcgg
     1801 ctgattcgga tcgagcccgg ctttcttgaa agcgtccttg ttgtagtaca gcacaggcgt
     1861 tgagctgttg aacggcatcg agatcagctc accgcttttg gcgtcgctgt aatagctggc
     1921 gatggtcggc acgaaggcct tttcgtcgag cggtacgccg gcttgcttga acacttcaga
     1981 gaccgggatc acggcctttt tcgcctgcat catcgtggcg gtgccgactt cgtagacttg
     2041 cagaatggcc ggcgcgttgc cgctacgata agcggcgatg ccggcggcga gcgtctggtc
     2101 gtaggtgcct ttgaacaccg gcacgatctt gtaatcgctt tgcgacgcgt tgaaggcgtt
     2161 ggcgatatcg ttcaggcgtt cgccgagggc ggcttccatg ccgtgccaga actggatttc
     2221 agtggcggcg tgggctgcct gactgatgcc gaagctcaat acggcggcca cggaaagcga
     2281 acggaacagc ggcttgcaac tcattgattt ggtctccttt gtcgaccgga gttagcgctt
     2341 tagcgcttac tccggtccca tgctttgcgg tcgaccggag tagcgcttta gcgcttactc
     2401 cggccccatg caacatgctt gttgaacgga acgggcgcgg acgggaatcg cgcgattcta
     2461 tttgtgttcg atgacaatcc ggcgtcggct gctggccggc cccccgcttc cggcaggcgc
     2521 cgaatgcggc aatttaacat cggctgtgac gaaacggaaa caatgcgcgt ctgaagccgc
     2581 cgggacctgc gacgtcaaga cccaacacgc gctgcttgtg cgcgggcatc atggacggtg
     2641 aggcggcggc gtgcttcgcg cagtcatgca tgtttcctga tgacattttt taataaggat
     2701 tccagatgct aagtgccggg cggttggcag tttgcgtttc gatagcaggc ttcatggcga
     2761 tgggttgtgc gttgacatct gagatggcgg acgccagagt gacaccggtg acatccttgc
     2821 cgaagatcat tgcgcatcgc ggcggcacag gcgacgcgcc ggagaacacg ctcgaggcga
     2881 ttcgccagtc aatcgcgcat catgccgacg agatgtggct gaccgttcag ttgagcaagg
     2941 acggcgtgcc tgtgttgtac cgtccggccg atctgtcggc attgaccaat gcaaaggggc
     3001 cagtctccga attcacggcg gcggaactcg cgcgcgtgaa cgccggctgg aatttcaaga
     3061 gcggcgacat ctatccctat cgcaatcacc cgatcggtat tccgaccttg cgcgaggcat
     3121 tgcgcacgat tccgctcgcc acgccgatca tcctggatat gaaagcgttg ccggctgggc
     3181 cgcaggctca ggccgtggcg caagtgctca acgaagaaag cgcgtggtcg cgcgtgacga
     3241 tctattcgac cgaagcggat tatcagcaga gcttcgccgc ctattcgcag gccaggctgt
     3301 tcgagtcacg cgatgcgaca cgcggacgcc tcgtgcgcgt gctgctcaac cagggctgcg
     3361 tggacgcgcc ggctgagcac accgccgccg cgttcgagtt gcaccggaca atgaccgtgg
     3421 tggaaaaatt tacgctgggc gaaggccgct ccgaggttca ggcaacgttg tggaccccca
     3481 caacggtcgc gtgtttccgg cagaagccgg atgtacggat cgtcgcgatc gcggtgaaca
     3541 atgcggccga ctatcggacg gcggcgtgtc tggggatcga tgcagtgctg gcggattcgc
     3601 cgcaaaggat ggctgcgatc aaggcaggta tggctatgcc gctgcggtgt gaggcgcaga
     3661 agtaggcata gaagtagcct cagaagtagg cgcagaagcg ggagcagaag cgggcgcact
     3721 tcggcccata aaaaaagccc gcatttcgcg ggccttctcc aagtacggcg gagttcagcg
     3781 atcaccggct accacctcgc gcggaggctg aacctcaaaa ccggcggggc tcagcattcg
     3841 ctggctgccg cctcgcgccg aggcaaacct caaacgttga acaggaagtt catcacgtcg
     3901 ccgtcgtgca cgacgtattc cttgccttcc gcacgcatct tgccggcttc tttcgcgcct
     3961 tgctcaccct tgtaggtgat gaagtcgttg aaggcgatcg tctgcgcgcg aatgaagccg
     4021 cgctcgaagt cggtgtgaat cgcgcccgct gcctgcggtg ccgtatcgcc gacatggatc
     4081 gtccacgcgc gcacttcctt cacgccggcc gtgaagtagg tttgcaggcc cagcaacttg
     4141 aaacccgccc gaatcacccg gttcaggccc ggctcttcca tgcccatgtc ggcgaggaac
     4201 acttccatat ccgcttcttc gaggtcggcg atttccgcct cgattgccgc gcacacggcg
     4261 acgaccggcg caccttcggc tgccgcgaac ttcgtgaccg cgtccagatg cgggttgttc
     4321 tggaaaccgt catctttcac gttggcgacg tacatggtcg gcttggcggt gatcaggcaa
     4381 aacggcttga gcgtggcctt ttcctcatcc gacagatcga gcgcgcgaac cggcttcgcc
     4441 tggtcgagtt gcgcgcggac cttttccagc acggcggcgt tcctggcggc ttccttgtcg
     4501 ttacccgact tggcggcctt cgaatagcgt gtcagcgcct tttcgacggt ggcgagatcg
     4561 gccagcgcca gttcggtgtt gatcacctcg atatccgaca gcggatcgat cttgttcgcg
     4621 acgtggatca cgttctcgtc ttcgaagcag cgcaccacgt gcgtaatcgc gtcggtttcg
     4681 cgaatgttcg cgaggaactg gttgcccaga ccttcaccct tgctcgcacc ggccacgaga
     4741 ccggcgatgt cgacgaattc caccacggcc ggcaagatgc gctccggctt gacgatttcg
     4801 gcgagcgctt tcagacgcgc gtccggcact tcgacgatgc cgacgttcgg ctcgatcgtg
     4861 cagaacggat agttttccgc ggcgatgccc gccttggtca gcgcgttgaa cagggtggac
     4921 ttgccgacgt taggcaggcc gacgatgccg catttgaggc tcatggaaat ccttcagtga
     4981 atcagtaagt tgcgtgatgc gctcgacgct gtttgcagga ttcgaaaagc cttcgcgggt
     5041 ggcgtggcgg aggaaacgca gcggagcggg acaagacacc agatcggggc gttgcctcga
     5101 cgattctggg cgagttttgt cacggaaagc gcaattgtaa cccgttcgga tggggcgacc
     5161 gggaagggcg cgggaaaccg tgctgggccg gaaatatccg gtgggaacat tgtaatatgc
     5221 gggtccgggg ccgctagagc cctattccgg ggaaacacga tgacaataaa taaatgggct
     5281 aggtggatat tgtgcgccgc gctgattggc gcgggcgtgg ttgcctggct ctcgccgaac
     5341 tacgaggaag cccaggccgc cactgatcgc cagacggtgg actgcatggt gaagcagctc
     5401 acgctggagg acaaacgcaa cattgcgcag ttcgccgata ccaatgattt cgactccctg
     5461 tggcgggtgt acgaccgcgt cttcccggac tgcgccgtac ggggcgatca gcgagaccgt
     5521 aaaagcgaac tggtgacgag cgcgtggcgc gtcctgtcaa ccgaccgtga tttcatgctt
     5581 ttgcgcgaaa cccatgcggc gcacgccgcg ctcggacgct aggtttccgc ccaaatgccc
     5641 catgcgtcga tccgtccccc gcaacccccg cggctataat gcgcggatga acgcacacca
     5701 ccagaccttt gatgccgccg tgatcggcgg cgggctcgtc ggcaagaccg cggcgctggc
     5761 gctgacgcaa ggcggactac gcgtggcact acttgcgcaa ccctgcgctg cactgcccgc
     5821 cggcgcggcc ttcgactcgc gggtctacgc gctgtcgtcg agttcgcagg cgctgctgga
     5881 gcggttgcgg gtctggcagg cgctcgattt gtcaaagctc gggcccgtct acgacatgcg
     5941 tgtctacggc gatgcccacg ccgaactcca tttttccgcg tttcaggcgt ccgtgccgca
     6001 actggcgtgg atcgtcgagt cgtcggtcat cgagcgtgca ctggatgccg cgctgcgttt
     6061 ccagccgaat ctcacctgga tcgacgcgcg cgcgcaggcg ctggacttta ccgccgacgg
     6121 tgcaagcgta ggtctcgcca acggcaatgt gctcgaggcc gatctggtgg tcggagcgga
     6181 cggcgcgcat tcgttggtgc gtgcgcagat cggctcgaag atgaaccggc gcgactataa
     6241 gcagaccggc gtggtcgcga acttcaaggc cgaaaagccg cacggcgaaa ccgcgtatca
     6301 atggttcaag gacggtgaaa tcatcgcgct gctgccgatg ccggacggcc acgtctcgct
     6361 ggtgtggtcg gcgcgcacgg aacacgcgga gcaactgatc gcgctcgatc cggcacaact
     6421 cgccgcggag gtcgaacgcg tgaccggcgg tcagttcggc gctttggaat gcatgacgcc
     6481 cgcgcaaggc ttcccgctcg cgctgcaaac cgtcgacaag ctggtggcgc cgcgcgtcgc
     6541 gctggtgggc gacgccgcgc acctgattca cccgctggcg ggccagggca tgaacctcgg
     6601 tttgcgcgat gtcgcggcgc tcgccgagac ggtcgcccgc aaggaagcgt tccgggatct
     6661 cggcgacatg gtcctgttgc gccgctacga acgcgcccgc cgcgaagaca tccgcgcgct
     6721 gatggtcgcc accgacggcc tgcaaaaact gttttcggcg cccgggccgt ttgccaaggt
     6781 gttgcgcaat accggcatgg cgtttgtggg cgcacagccg ttcatcaagc gctggctggt
     6841 gtcggccgcg ttggggtaaa gcgcccgaac ccgcgtgccc gaccggcgag gcgggtatca
     6901 tgaacggatg gacgccattc tgggcaccaa tctcgtcaca cccgaaacgt cgaaaaacgc
     6961 gcaccgcgcg cgcttgaact ccaggaattc agcatgaaaa aatcctttcg catcgccgcc
     7021 gtcgtgctcg cgatcgccgc cgtggcaatc ggctgctccg cgcaggccga tcaaaccacc
     7081 gacaagctca aagccacgct gcaaacgcgc ctgaacgacg tgacgatcaa gagcgtctcg
     7141 aagtcgccga ttgccgggct ctacgaagtg aacctcggca cgcagatcat ctatagcgat
     7201 gcgaacggcg actacctcat gcttggcgac atggtcgacg cgaagacccg caagaatctg
     7261 acggaagcgc gtctgtcgga gaccaaccgc atcgacttcg cgagcctgcc gttcgcggac
     7321 gcggtgaagg tcgtgaaggg caacggcgcg cgcaagatcg ccgtgttctc cgatccgaac
     7381 tgcccgtact gcaagcagct cgaaacgtcg ctcaagtcga tcgataacgt cacggtctac
     7441 accttcctgt acccggtgct gtcgccggat tccaccgtca agtcgaagtc gatctggtgt
     7501 tcgcccgacc gcgcgaaggc gtgggaatcg tggatgcagg atcatcagtc gcccacggca
     7561 gccggcacct gcaacaccgc cgcgatcgac aagaacctgg cgcttggccg ggccatgggc
     7621 gtcgacggca cgccgaccgt atttctggcc gacggccgcc gcctgcccgg tgccgtgccg
     7681 gcggaccgcc tggacaggga aatgtccgcc gtgcactgag cacgcaacgc aagtcgaaga
     7741 aaggaggcgc gcagtgcgcc tcctttcaca tcgagccccg aacatccccg cctgaaacca
     7801 tctaatcgcc gccgatgaag ccgatccgct acaccatcgt tcccaagcaa cccgccgccc
     7861 atctgttcga agtcaccgtg actgttgccg atcccgatcc ggccgggcag cgtttcatgc
     7921 tgccggtctg gatcccgggc agctacatgg tgcgcgagtt cgcgcgcaac atcgtcacgc
     7981 tgcgcgccgt caacgaagcg ggccgcaagg tgcgcgtcga aaagaccgac aagcacacct
     8041 ggcaggccgc gccggtcaag ggcgcgttga cgctgcgcta tgaagtgtat gcgtgggatc
     8101 tgtcggtgcg cgccgcgcat ctggacgata cgacgggttt tttcaatggc accagcgtgt
     8161 tcctgtcgcc gctcggccat gaggaagccc aatgcgtggt cgatatccag aagccggaag
     8221 gcgcggcgta tcgcaactgg cgcgtcgcga ccgcgttgcc ggaagcgcgc gggacgaagc
     8281 gctacggctt cggcgaatat cgcgcgcaga actacgatga gctggtcgat catccagtca
     8341 cgttaggcga gttcgcgctg gcgagcttca aggcgcacgg cgtaccgcac gacatcgtga
     8401 tcgcaggccg tgtgattggg ctggatatgg cacgtctgtc cgccgatctg aagcgcatct
     8461 gcgaagcgca gatcgcgttg ttcgagccga agtcgaagaa ggcgcccgtg gatcgctacg
     8521 tgttcatgac gcaggcggtc acggatggtt acggcgggct ggaacatcgc gcgtcgacgg
     8581 cgctgatctg caaccgcacc gatttgccgg tggaaggccg cgataccccc accgaaggct
     8641 accgaaccta tctcggtctg tgcagccacg aatacttcca cacctggaat gtgaagcgca
     8701 tcaagccggc cgtgtttgcg ccgtacgacc tgagtgtcga aaactacacg ccgctgctgt
     8761 ggttgttcga aggctttacc tcttactacg acgatctgat ccttgtgcgc acgggcctga
     8821 tctcgcaaga cgactatctc ggcatgctcg gcaaggtaat cggcggggtg ttgcgcggca
     8881 gcggccggct gaaacagacg gtggccgaaa gctcgttcga cgcgtgggtc aaatactatc
     8941 ggcaggacga aaatgcgccg aacgcaatcg tcagctatta caccaagggc tcgctcgtcg
     9001 cgctcgcatt cgatctgacg atccgcgcgc agacgagcaa ccgtaaatcg ctcgacgacg
     9061 tgatgcgtct cttgtggcag cgctttgggc gcgacttcta tcgcggtaag cccgttggcg
     9121 tcgaagcaag cgagatcgag gcgctttttg ccgaagcgac gggtgcgcaa ctgggcgagt
     9181 tgttcgccga agctgtgcac agcacgcacg atctgcccct cgacacgctg ctcgcgccgt
     9241 ttggcgtcac gctcgcgccg gaactggaca agaacggtaa gccgtcgctc ggcgcgcgca
     9301 tgcgcagcgg cgcggattgc acgctggcag cggttcacga aggcagcgcg gcgcagaaag
     9361 ccggactttc ggcgggcgac gtcctggtcg cgcttgacgg tttgcgcgtg accggctcga
     9421 atcttgacgc cctgttgtcg cgctatctgc cgggtgcaaa agtcgaagtt cacgccttcc
     9481 gccgcgacga gcttcgcacc gcgcaagtca agctggacgg gtctgaggtg gcgcgctaca
     9541 agctcaccgt gagcgataaa cagcctgcgg cgcgcaaggc gcgggagcgc tggctggcaa
     9601 gctgatcgtg attctggtcg tcgtaatcga cagcgccagg cgggggattg ttctaccagt
     9661 gcaacaatcc ttcgctgccg gacggctttt tcccgggccg gtaaaaaaac acaatggctc
     9721 cactggcgcc acactgcgcg cacctactgg agtcaaccat gaccacgatc ctgcaaatca
     9781 actcggcagc ccgttcgcaa ggcgcgaact cgacgctgct cgtcaacgag ctgaccgcaa
     9841 agctgcaaca atcgaatccg ggcgcgcaag ttgtcgtccg taacctgcaa gctgaaccgc
     9901 tgccgcacct ggacgacgcc gtcctcggcg cgttcttcac gccggcagac cagcgcacgc
     9961 cagaacaagt cacgatcgcc gcgcgcagcg aagcgctgat cgccgaactg caagccgccg
    10021 acatcgtcgt gatcggcgca ccgatgtaca acttcggcat ctcttcgcaa ctgaagacgt
    10081 acttcgactt catcgcacgc gccggcatta cgttccagta cacggcgaat ggtccggtag
    10141 gtctcgtgga gggtaagaag gtgttcgtgg tatcggctcg cggcggcaag tatctgggca
    10201 ccccgaacga cagccaaacg ccgtatctga agagcttcct gggtttcctc ggcatgaccg
    10261 atgtgagctt catctacgct gaaggcctga acatgggccc ggaggctgcg tccgccgcat
    10321 tggctggtgc gcgtgaagcg atcgctgctg cgtaaggtgt gaggtttgtc ccgcgccggg
    10381 tgattgcggc gcggcggaat gaaaaaacgc cacggggtgt gattccgtgg cgtttttgtt
    10441 ttttgcggct gcgatcctgg gttactggtt atcgggtttc ggtctcggtt tccagtttcc
    10501 agtttccagt tttcggtttc cggtttccga tctttggtca gtgtcggtgc gcgcgtgcct
    10561 cgcgcgattg acgtcttatg ccagcatctg cgccgcgtcg ggcaagcgcc aatcgatcgg
    10621 tgtgcggccg tgcttcacga gatattcgtt cgccgacgca aagtgcccgc agccgagaaa
    10681 gccgcgatgc gccgacagcg gcgacggatg cggcgcctcc agcacgtaat gcgacttgcc
    10741 accgagcagc gcgcgtttgg cttgtgcatg cgcgcaccac agcatgaaca cgagaccgtc
    10801 gtgacgcacg gccagttcgt ggatcagcgt gtcggtgcat ttctcccagc cgcgtttcgc
    10861 gtgactcgcg gctgaatcgc gctcgacggt cagtaccgtg ttgagcagca atacgccttg
    10921 cttggcccaa gtgtcgaggc agccatgacg cggcggctcg tggccgaggc tggcggcaat
    10981 ttccttgaag atgttgcgca gcgacggcgg cgtgcgcacg ttcggcgcca ctgaaaacgc
    11041 cagtccgtgc gcttgtggcg tgccgcggtc ttcgccgtgg tacggatcct ggccgaggat
    11101 cacgactttg acttcgtcgg ggctggtgag gcgcagggcg cggaacacgt cggtgggata
    11161 gacggttttg ccggcagcgc gctctccgtc gacgaaacgg cataacggcg cgtaggcgtc
    11221 gctttcgatg aacggtttga ggtgtgcgcg ccaggccggg ggcagggcgg cgaactgggc
    11281 ttcgagggtc ggcggcggcg agtcagcggc aacggatgtc tgctgctgcg ctgttgcgct
    11341 ggacggcgtg gccgaggcgt cgccgaacaa cgaggcttgc gacgaattgg agcgggtgcg
    11401 ggaagctgag gtcatggcga ggaagtgtcg cagcaaacgc gggctagctc aagtttgtgc
    11461 tgcttgagcg ctgcgcattg tgctggcgtg cggcggtatt gcgattggac ttcagcgcgt
    11521 ggtgcaatcg gcgacacagc tgcggtgaca caactgcggc gaaacgcaaa cggcgaaacg
    11581 cgaacggcga aacgcaaacg tcgcggcaca aacccgcgcg ccgacggctt agtctgcccg
    11641 caattgatag ccgcgctgag ctttgctgat gttgtccggc gcaagcccgg ccacctgttt
    11701 gcccaattct ttcgccagcg tatccagcac cgcttcgtcg ccggacttca gttccagttc
    11761 gacttcagag atcggcgcgc ggcgcatttc gccattcacc tccgcgataa cgtcgccctg
    11821 gtcgatcgcg gcttcgactt cggagccgtc cagagcgaca ttccacagcg tgcgcgtgaa
    11881 attcgtgcga aacagttcga tcagttccgg cgcagcccga cgcagggctt gcgcggcggc
    11941 gggttcgtca cactggcgca gcaaggcttc gatctccagc t
//
LOCUS       DATASH010000920         3433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_353031,
            whole genome shotgun sequence.
ACCESSION   DATASH010000920 DATASH010000000
VERSION     DATASH010000920.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353031"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VHV99_24590"
     CDS             complement(<1..353)
                     /locus_tag="VHV99_24590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEX3638599.1"
                     /translation="MKAAAAHREPAPQADQARADGASPYATISDPHRSDIANVTRRLL
                     ILFALVAGLVAACGLTYSIAWQRGIDNLRHNAAVRVDRTTSALKSTLERYESLPYLLA
                     EHPIVQDVLVNPSPA"
     gene            complement(412..1692)
                     /locus_tag="VHV99_24595"
     CDS             complement(412..1692)
                     /locus_tag="VHV99_24595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HEX3638600.1"
                     /translation="MKKPIHKVLYVQVIVAIIIGIALGHFSPDFAVDMKPLGDGFIKL
                     IKMVIGPIIFCTVVTGIAGMEDMKKVGRVGGKALLYFEVVSSFALVLGLIATHVLKPG
                     VGFNIDPATLDGKAVASYAAKAHGQTTVDFLMHIIPDTLVSAFAQGEILQILLIALLF
                     GAVLATAGEKGKVVTNFIDGLSHVLFGVVRIITKLAPIGAFGAMAFTIGKYGIGSLLP
                     MLKLIGTFYLTSIVFVVVVLGIIARVVGFNILRFVGYIKEEMLIVLGTSSSEAALPQL
                     MLKLEKLGCSRSVVGLVVPTGYSFNLDGTNIYMTMAVLFIAQATNTDLTWTQQLTLLA
                     VTMLTSKGASGVTGAGFITLAATLAVVPTIPLSGMVLILGIDRFMSECRALTNIVGNG
                     VATVVVSAWEKELDRNKLNAALRGDVAVKEPAGA"
     gene            complement(2134..3204)
                     /locus_tag="VHV99_24600"
     CDS             complement(2134..3204)
                     /locus_tag="VHV99_24600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3638601.1"
                     /translation="MAFLEIENLHKAFGANAALHHFDMKIERGEFITFLGPSGCGKTT
                     VLRMIAGFETPTRGTIRLDNKDVTHLRTRQRKVGMVFQSYALFPNMTVADNIGFGLKI
                     THRPQAEIKKRVEEMLQLIKLPHLGERYPWQLSGGKQQRVALARALAGKPQVLLLDEP
                     LSALDAKIRISLRQDIRALQRELGITSIFVTHDQEEALSISDRIVVMNEGRVEQIGSP
                     LEIYNYPRTRFVASFVGTLNILAGHVIDPKTGKMAVDGQELTTTQELPADDAGKQRML
                     ALRPEAIVLEPAAPGRNTLNATVEEVNFLGAVVRIRTRVKDAVISLDVFNDPNRGLPE
                     RGQPVSLGFSHENLLVLEEGGA"
     gene            complement(3275..>3433)
                     /locus_tag="VHV99_24605"
     CDS             complement(3275..>3433)
                     /locus_tag="VHV99_24605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3638602.1"
                     /translation="DRPAFGPYLQLIGANRAYEPSALAISAFVMTWASMGLIQVFGSA
                     RALSGQKI"
BASE COUNT          673 a         1067 c         1021 g          672 t
ORIGIN      
        1 ttggctggac tgggattgac cagcacgtct tgcacaatgg gatgttcggc cagcagataa
       61 ggcagcgact cgtagcgctc gagcgtgctt ttcagcgcgc tcgtagtgcg atcgacccgc
      121 accgcggcgt tgtgccgcaa attgtcgatg ccgcgctgcc atgcgatgct gtacgtgagc
      181 ccgcacgccg ccacgagccc agccacgagc gcgaagagga tcagcaggcg gcgcgtcacg
      241 ttggcaatat ccgatcggtg aggatcagat attgtggcat aaggcgacgc gccgtcggcg
      301 cgagcttggt cggcctgcgg cgcgggttcc cggtgcgcgg ccgccgcttt catctgtggg
      361 cgtccgcggc cgtgatcggg agggtggcgg aagtcgtgac cgttgcgcgg cttaagcgcc
      421 agccggctct ttaaccgcga cgtcgccccg caatgcagcg ttcagcttgt tgcggtccag
      481 ttccttttcc catgccgaca ccaccaccgt cgccacaccg ttgccgacga tgttcgtcag
      541 cgcgcggcat tcgctcatga agcggtcgat tccgagaatc agcaccatgc ccgacagcgg
      601 aatggtcggc accacagcga gcgtggcggc gagcgtgatg aagcctgcgc ccgtcacacc
      661 gctcgcgccc ttcgaggtca gcatcgtcac cgccagcagc gtgagctgct gcgtccacgt
      721 gaggtcggtg ttggtggcct gggcgatgaa cagcaccgcc atcgtcatgt agatgttggt
      781 gccgtcgagg ttgaacgagt aaccggtcgg caccacgagg cccaccaccg agcgcgagca
      841 gccgagtttc tcgagcttca gcatcagttg cggcagcgca gcttcggaag agctcgtgcc
      901 gagcacgatc agcatctctt ccttgatgta gccgacaaaa cgcaagatgt tgaagcccac
      961 cacgcgcgcg atgatgccga gcacgaccac cacgaacacg atcgacgtca gatagaacgt
     1021 gccgatcagc ttgagcatcg gcagcagcga gccgatgccg tacttgccga tcgtgaacgc
     1081 catcgcgccg aacgcaccga tcggcgcgag cttcgtgatg atgcgcacga cgccgaacag
     1141 cacatgcgaa agcccgtcga tgaagttcgt gacgaccttg cccttctcac cggccgtggc
     1201 cagcaccgcg ccgaacagca gcgcgatcag caggatctgc aggatctcac cctgtgcgaa
     1261 cgcggacacg agcgtgtccg ggatgatgtg catcaggaag tcgaccgtgg tctggccgtg
     1321 cgccttggcg gcgtacgaag cgaccgcctt gccgtcgagc gtggccgggt cgatgttgaa
     1381 gccgacacca ggcttcagca catgggtcgc gatcaggccg agcaccagcg cgaacgacga
     1441 gacgacttcg aaatacaaca gcgccttgcc gccgacgcgc ccgaccttct tcatgtcttc
     1501 cataccggcg atacctgtca ccaccgtaca gaagatgatc ggcccgatca ccatcttgat
     1561 cagtttgatg aagccatcgc cgagcggctt catgtcgacg gcgaagtccg gcgaaaaatg
     1621 gccgagcgca atgccgatga tgatcgccac gatcacctgg acgtacagca ctttgtggat
     1681 aggtttcttc acgatgtttc ctgcgatatg ggtgagctca aggcctgcca cgctgcgcat
     1741 catgcgaacg acgagtcgtc acgcctgtgc acggcataag gccgcgacaa gacgacgaat
     1801 tcagaaatac cggggaggga aatcagacat cgttgtctcc ttgctttagt tgtcggaccg
     1861 aggcggccgc gtcttctaaa tcgcaaggtc gatgccagct tgatgcacgg cgaatcgctt
     1921 tgatttttat aggttttttg gtgcgctacg gcgtgcactg tccgagattc cggaaatccg
     1981 gacgaggggt gtcggggaat ccggaatcct tcaggcgacg acctcgggaa aacccgcgga
     2041 gccttttggg ccgcgcgttt cgcgcaatcc ggaggtcaca attctggctt ctttgcgtcg
     2101 ctttcgctcg cttcaggtgc tgctgtccgg cttctatgcg ccgccttctt ccagcaccag
     2161 caggttctcg tgcgagaagc cgagcgacac cggctgtccc cgctcgggca aaccgcgatt
     2221 cggatcgttg aacacatcga gcgaaatgac cgcatccttc acgcgcgttc tgatacgcac
     2281 caccgcgccc aggaaattca cttcttcgac ggttgcgttc agcgtgttgc ggcccggcgc
     2341 agccggctcc agcacaattg cttcgggccg taaggcgagc atgcgttgtt tgccggcgtc
     2401 gtctgccggc agttcttgcg tggtggtcag ttcctgaccg tcgacggcca tcttgccggt
     2461 tttcgggtcg atcacatgcc ccgccagaat attgagcgtg ccgacgaacg acgcgacaaa
     2521 gcgcgtgcga ggatagttgt agatctccaa aggcgagccg atctgttcga cacgcccctc
     2581 gttcatcacg acgatgcggt ccgaaatcga caacgcctct tcctggtcgt gcgtcacgaa
     2641 aatcgacgtg atgcccaact cccgctgcag cgcccgaatg tcttgccgca acgaaatgcg
     2701 aattttcgca tcgagcgccg acagcggttc gtcgagcaac agcacttgcg gcttgccggc
     2761 aagtgcgcgc gccaatgcca cacgctgctg cttaccaccg gacaattgcc acggataccg
     2821 ttcaccgaga tgcggcagct tgatcagttg caacatctcc tcgacgcgct tcttgatctc
     2881 cgcctgcgga cggtgcgtga tcttcagtcc aaagccgatg ttatcggcca ctgtcatgtt
     2941 cggaaacagc gcatacgact gaaacaccat gccgaccttg cgctgccgcg tgcgcaaatg
     3001 cgtcacgtcc ttgttatcca gccggatggt gccgcgcgtc ggcgtttcga agccggcgat
     3061 catccgcagc acggtagtct tgccgcaacc ggacggtcca aggaacgtga tgaattcgcc
     3121 gcgctcgatc ttcatgtcga agtgatgcag cgccgcgttc gccccaaagg ccttatgcag
     3181 attttcgatc tcaaggaatg ccatgattcg ctgcctcagt ggtcaaactt agtggtcaag
     3241 cttagtggtc aaactcagtc gtcaaactta gtcgtcaaat tttctggcca ctaagcgcgc
     3301 gggccgagcc gaatacctga atcaagccca tcgacgccca cgtcatcaca aacgcgctga
     3361 tcgcgagcgc cgacggttca tacgcgcggt tcgctccgat caattgcaga tacgggccga
     3421 acgcggggcg gtc
//
LOCUS       DATASH010000921         1833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2728650,
            whole genome shotgun sequence.
ACCESSION   DATASH010000921 DATASH010000000
VERSION     DATASH010000921.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2728650"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1833
                     /locus_tag="VHV99_24610"
     CDS             <1..>1833
                     /locus_tag="VHV99_24610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX3638603.1"
                     /translation="TFARAKVDDGKLRGVALESLQDGQPQVRQSIDRASHFLSLVSLL
                     TALLAAVAIAMASHRFARRHLDGCAAMRCLGVSQGTLRSLFIGEFLAVGLLGSAVGVV
                     LGFGGHLVLLDWLGTLVDVELPPPSVWPALQGIATGLVLLLGFAVPPLLPLTRVPPVH
                     VIRRELGDAGRVAWIAYGVGVLLFAGLLVLAAGEWKLGGIVAGGFAGGLVLFGGIARV
                     ALWAAARFVRSERVGAGVGWRYALASLERRSGASALQITALGIGLMCLLLIAMTRSDL
                     IAGWRQSTPPDAPNEFLIDVQPDQRAGVTDYLKQHGQGDAVLSPMVRGRLVAVNGKPV
                     SPDNYEKADAKRLVDREFNLSYTTELPSDNRVVGGDWYGTDGKVQVSIEEGLAKIIHV
                     KLGDTMRFDVAGMQVEGPVTSLRKVDWNSFKVNFFVLMPPPSISDLPATFITSFHLPP
                     GQQSFIDGLINKYPSVTAIDIAPILAQIQRVLEQVIGAVQFLFVFTLAAGVLVLYAAL
                     AGTRDERVHESALLRALGASHKQVRSVQTAEFVAVGALSGLMAALGAQGIGYVLATRV
                     FEFHIDFNPWLVPAGIVAGVVCSGVGGWMSLRHVLSRPALQSLR"
BASE COUNT          254 a          543 c          656 g          380 t
ORIGIN      
        1 ccacgttcgc ccgcgccaag gtggacgacg gcaagctgcg cggcgtcgcg ctcgaatcgt
       61 tgcaggatgg ccagccgcaa gtgcgtcagt ccatcgaccg cgccagtcat ttcctctcgc
      121 tcgtctcgct gctcaccgca ctgctcgcgg cggttgccat tgcaatggcc tcgcatcgct
      181 tcgcgcgccg gcatctggac ggctgcgcgg ccatgcgctg cctcggcgtg agccagggga
      241 cgttgcggtc tctgtttatc ggcgaattct tggctgtcgg gttgctgggc agcgcggtgg
      301 gtgtcgtgct cggcttcggc gggcatctgg tactgctgga ctggctgggg acgctcgttg
      361 atgtcgaatt accgccgccc agcgtctggc ccgcgttgca gggtattgcg accggactgg
      421 tgctgttgct tggtttcgcc gtgccgccgt tgctgccgct gacccgtgtg ccgccggtcc
      481 acgtgattcg ccgcgagctt ggcgatgccg gccgggtagc gtggatcgcc tacggcgtgg
      541 gcgtgctgct gttcgccggc ctgctcgtgc tcgcggccgg cgaatggaaa ctgggcggga
      601 tcgtggcggg cggattcgcg ggcggtctgg tgctgtttgg cggtattgcg cgcgtggcgc
      661 tatgggcggc cgcgcggttt gtgcgcagcg aacgcgtggg cgcgggagtg ggttggcgat
      721 acgcgctggc gtcgcttgag cggcgcagcg gcgcgagcgc gttacagatc acggcgcttg
      781 gcatcggcct gatgtgcctg ctgctcattg caatgacgcg cagtgacctg attgcgggct
      841 ggcggcaatc gacgccgccg gacgcaccga acgaatttct catcgatgtc caacccgacc
      901 agcgcgccgg cgtcaccgat tacctcaaac aacacgggca gggtgacgca gtgttgtcgc
      961 ccatggtgcg tggtcgcctg gtcgccgtga acggcaagcc ggtcagtccc gacaattacg
     1021 aaaaggccga cgcaaagcgt ctggttgacc gcgagttcaa cctctcgtac accaccgaac
     1081 tgcccagcga taaccgcgtg gtcggcggcg actggtacgg cacggacggg aaggtgcagg
     1141 tatcgataga agaagggctc gcgaagatca tccacgtgaa gcttggcgac acgatgcgtt
     1201 tcgacgtcgc cggcatgcag gtcgaagggc cggtcacgag cctgcgcaag gttgactgga
     1261 attcgttcaa ggttaacttc tttgtgctga tgccgccgcc gtccatcagc gatctgccgg
     1321 ccacgttcat cacgagtttc catttgccgc cggggcagca gagtttcatc gatggcttga
     1381 tcaacaagta tccgagcgtg accgccattg atatcgcgcc aattctcgcg cagatccagc
     1441 gcgtgctgga gcaggtgatt ggcgccgtgc agttcctgtt tgtcttcacg ctcgcggcgg
     1501 gcgtgctcgt gttgtacgcg gcgctggccg gcacgcgcga cgagcgtgtg cacgagtccg
     1561 cgttgctgcg tgcgctcggg gcatcgcata agcaggtgcg ctcggtgcag accgcggagt
     1621 tcgtggccgt gggtgcgttg agcgggctaa tggcggcgct tggtgcgcag ggaattggct
     1681 atgtgctggc gacgcgcgtg tttgagtttc acatcgattt caacccgtgg ctcgtgcctg
     1741 cgggcatcgt ggcgggtgtc gtgtgttcgg gcgtgggcgg ctggatgagc ctgcgccacg
     1801 tgttgtcgcg gcccgccttg caatcgttgc gcg
//
LOCUS       DATASH010000922        12927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1147597,
            whole genome shotgun sequence.
ACCESSION   DATASH010000922 DATASH010000000
VERSION     DATASH010000922.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 12927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12927
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1147597"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1366)
                     /gene="fliF"
                     /locus_tag="VHV99_24615"
     CDS             complement(<1..1366)
                     /gene="fliF"
                     /locus_tag="VHV99_24615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HEX3638604.1"
                     /translation="MDSSANSLINPDARMGLAGAQPGGAAAGAGQAGGAADLGGLGAN
                     LGGNFGQRLSGLAQMRGNPRAPLIFAVALLVAIVAGLFLWSRAPDYKVLYSNLSDRDG
                     GAIITALQAANIPYKFSDSGGAILVPAEQVHEMRLRLASQGLPKSGSVGFELMDNQKF
                     GISQFAEQVNYQRALEGELERTIESISSVKSARVHLAIPKPSVFVRDKEAPSASVLVN
                     LYPGRALDEGQVLAITHLVASAVADMPVKGVTILDQDGNLLTQSSTGSGLDASQLKYR
                     QQIERNTQQRIDAILEPLFGSGNAHSQVSADIDFSHSEQTSENYGPNGSPQQAAIRSQ
                     QSSTATEMSQAGASGVPGALSNQPPQPVSAPITAASGTAGVTTTPVSDRKDSTTNYEL
                     NKTVQHLEQPMGGIKRLSVAVVVNYQRVVDARGHATMQPVTADKLAQVNQLVKDAMGF
                     DATRG"
     gene            1604..1945
                     /gene="fliE"
                     /locus_tag="VHV99_24620"
     CDS             1604..1945
                     /gene="fliE"
                     /locus_tag="VHV99_24620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HEX3638605.1"
                     /translation="MTLPVNALSSALQQMQSMAAQAAGSSTAAGVADASGAASAGGFA
                     SALKASLDKISSDQTKAVGESQAFELGASNVSLNDVMVDMQKANVGFQFGLQVRNKLV
                     SAYSDIMQMSV"
     gene            2242..2679
                     /gene="fliS"
                     /locus_tag="VHV99_24625"
     CDS             2242..2679
                     /gene="fliS"
                     /locus_tag="VHV99_24625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HEX3638606.1"
                     /translation="MLSPGDSGANAYAHVGVETEVMGASPHRLIVMLYQGARRAIAQA
                     RTHIQMGNVPAGGEAIGKAIQIVESGLQQSLNVEAGGEIAERLNALYSYIARRLLEAN
                     IKQSEAMLVEVDGLLATLEEAWSGIAPEIARMAAQPAAAESMR"
     gene            2676..3002
                     /locus_tag="VHV99_24630"
     CDS             2676..3002
                     /locus_tag="VHV99_24630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HEX3638607.1"
                     /translation="MTSNVEYFARYEAIAAISRRMLTAARRALWSDLAHLQEEYRNLV
                     ESLRDAETGVKLSDAERMRKYALICQILADDAAIRDLANPRMANLSALFAGRPTRVLK
                     ELYGAR"
     gene            3085..4494
                     /locus_tag="VHV99_24635"
     CDS             3085..4494
                     /locus_tag="VHV99_24635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HEX3638608.1"
                     /translation="MNGIDTVVSSLLANRVDSLLNLAPSSVTASQTGAAGVDSAPITV
                     TPLTPPATPPASAQTALSAVALTLNAIINSGGEATPAVLGQVPIWPAAPALDVEPGGL
                     PLFDTPAASSTNPNAAAATATANVAAAQVPVAALAAALEQTVGVSGLFYESHLAQWLA
                     GQRPPAALADEAQNKLVAAATQLPLDWASDADQASSSNAAPRQGTGTGTGPNGPANGA
                     PDGNAAARATPSIQTAQAARFVAGEVLASSLSDLNGLSAHASVHSAAAQLADDGSSQN
                     SQSMAAAVHPATVPLVRQQLDLLATGQFRWTGEAWPGAKLDWTIEQEGDEWDRSGGGT
                     ASEEDHPWRTRLTLSLPTLGTVDADLTLTGSRLVARVQASPAGAARMAMQGESFRQRL
                     AAAGIELNGLTIREIGGGAPANAAGAVGAAATAQAAASAYARSAASASTADKVPAGRR
                     DARAGRPGGAPLDDFDWDM"
     gene            4494..4859
                     /locus_tag="VHV99_24640"
     CDS             4494..4859
                     /locus_tag="VHV99_24640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HEX3638609.1"
                     /translation="MSRRHRRSAAALVYDAQGSDPAPRVIAKGYGMLAELIVQRAKEA
                     GLYVHEAPEMVSLLMQVDLDTRIPPELYQVVAELLAWLHRLESGAEPEPESAAAGVQD
                     VIDVVATDITDVSGGAVGR"
     gene            complement(5053..6471)
                     /locus_tag="VHV99_24645"
     CDS             complement(5053..6471)
                     /locus_tag="VHV99_24645"
                     /EC_number="1.2.1.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010100937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050269 - coniferyl-aldehyde dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006081 - cellular aldehyde metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coniferyl aldehyde dehydrogenase"
                     /protein_id="HEX3638610.1"
                     /translation="MKNDLPEVAALEALLRDQRHAYLRAPYPSWESRAAHLKALRKVM
                     LDNRDALADAMNADFGNRAKQEVLLAEFLLIKGEIDAALRHGKRWMKAQRRSTNKWLM
                     PARAKVVPQPLGVVGIIVPWNYPVLLAAGPLISALTAGNRAIIKMSELTPRTSALFEQ
                     LIGQAFARDHVAVVNGDAALGAAFSAQPFDHLLFTGSTRVGHEVMRAAAEHLTPLTLE
                     LGGKSPAIIGLHARFDNAVDNLIAGKTLNAGQTCVAPDYVLVPRGKEQAFIARARARM
                     AKMYPGFAHNLDYTSIISERHFARLQRLADEAQAAGAQLHMLTDSTPDEASRRFPLIA
                     VTNAPDESALMQEEIFGPLLPIVSYDTLDDAIAWINARPRPLALYLYADDTATIERVT
                     RETIAGGMAINETLMHLACESLPFGGVGASGMGAYHGYEGFVTFSKMKPVLTQARLNA
                     RGWISPPYGKRVNALLKLMMRF"
     gene            complement(6673..8358)
                     /locus_tag="VHV99_24650"
     CDS             complement(6673..8358)
                     /locus_tag="VHV99_24650"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HEX3638611.1"
                     /translation="MKAHSFGRTHDVTNQAPPLADYNLFSSDVALSAALEREGAAWHR
                     EGLQRHGAALTTPETLALAELANRHTPELFTHSPRGERIDALEFHPSWHTLLSLLRRE
                     GLHALPFSEPQRGAMVARCAGYFLHAQLESGSLCPLTMTFASIPVLQREPALFDTLRD
                     KLYTREHDPRDLPLAQKTSAMIGMGMTEKQGGSDVRSNQTRAYATRGSGRGAEYRLVG
                     HKWFFSAPQCDAHLVLARTDDHEGLSCFFVPRFAPDGSKNAVQIQRLKDKLGNRSNAS
                     SEVEFLDAFGIMIGDEGRGVPTIIEMANYTRLDCVIGSAALMRAALVQAIHHARHRSA
                     FGRHLADQPLMRNVLADLALESEAATVLLMRLARAFEASADASSSAPAERAWRRIVTP
                     ASKYWVCKRTLEFTGEAMEVWGGNGYVETGPMARFYREAPVNSIWEGSGNVMCLDVLR
                     AMEREPEAAQALFAAWQADAQGHPAVSAALGKLAATLNGAPDTRESSARRIAQQIVLI
                     AQATLLVKHAPPEVAEAFIATRLADGCGESGRVYGTLPVTFDHAAIIERAFPV"
     gene            8914..10656
                     /locus_tag="VHV99_24655"
     CDS             8914..10656
                     /locus_tag="VHV99_24655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX3638612.1"
                     /translation="MSINVIQLIQSALTDSVVRQLAARFGLPPDATQKVLATTGPALV
                     AGLMQKGATLDGARSLFATILAPEVNGHIAEQLPQLLGTTTGVSQLEGVGRQLLERAL
                     DRRVDTLSDEVATQTGVPAHATHAMTGIVGATLLGLLKRHFLEGQGNVGHLPTLLGHQ
                     LPAVAPHLNDRLMTGLGLSGLGAFTGNILSQLKAVSAHIDHPTPAAKPVAEALSSIRV
                     PADAVVREERRPRKWLWWLLAAVAAVLAFLFLRGCHSEQSAERSNAEAAVTAQPASAP
                     VEASAAAAATPAASDVAASTPAAAAAASAASDAQLPAAAPAPTRDSVLSLKVDAGGKP
                     TLTATLGSEAEKTQLIEALTRRFGADRFVANVTIDQDTKPADWLAHLDGLLPLMALPG
                     AEVKVDGAHIELSGSAANARLGWLDKLKSLFGASYQIGSFNVEQAVANATETFRGAIK
                     GLLTPDSPCAAADVVKVLNLQVINFTSASARVPASAIEDLNQSAQVLNACARNGKTAK
                     LEVAGYSDNVGGAQANLQLSKKRAEAVRAYLVKTGVPADSLVAQGYGDAQPVASNDTA
                     SGRFANRRIEFVAQ"
     gene            complement(10813..11769)
                     /locus_tag="VHV99_24660"
     CDS             complement(10813..11769)
                     /locus_tag="VHV99_24660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX3638613.1"
                     /translation="MELRALRYFVEVVRQQSFTVAAEQMFVTQPTISKMVKSLEDEIG
                     SPLLLRDGRQMVLTDAGRIVYQRGQDVLAAHAQLQAELNDLGTLGRGELTIGIPPMGG
                     SLFTPAIAAFRQRYPKIELKLFEQGARAIEAALIHGELELGGVLQPVDPENIEVLPMT
                     RQLLWLVARTGSRWDDLHEVPLAELAHEPFVFYGESLALNDVVVNACRTAGFAPTIVG
                     RSGHWDFMAALVLAGVGIALLPAPYCQRLDAAQFTCRPVVEPEIPWEMAIGWRRNGYL
                     SHAARAWLEVARETLPGQAGDDFMVGPGIGISGITAPSQTPS"
     gene            11993..12790
                     /locus_tag="VHV99_24665"
     CDS             11993..12790
                     /locus_tag="VHV99_24665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEX3638614.1"
                     /translation="MTPISIIIPCYNGAATLARALQSCLIQPEAAQIIVVDDGSTDAS
                     AELVAYCGSLDERVGLLRMPVNGGAARARNWGAMHASHSLLAFLDADDEYLPGALAAA
                     SAFLVQNPREVSVRLDVEYTDFPADIVSHAHFDKHAATLSNTVPSSLIIRRAVYAALG
                     GFPMDDAFRRIGGEDGAFSWALREIFGNRRLDDVKRVRMHYHAGIHAERYFRIALGMR
                     KPDPADLDDAFRFSRQFVETARAGIEQLRAASVAGEATAAMIPNQAA"
BASE COUNT         2188 a         4196 c         4302 g         2241 t
ORIGIN      
        1 cgccgcgcgt ggcgtcgaag cccatcgcgt ccttcaccag ttgattgacc tgggcgagtt
       61 tgtcggcggt gaccggttgc atcgtcgcgt ggcccctggc gtcgaccacc cgctgatagt
      121 tgaccaccac ggccaccgac agacgcttga tgccgcccat cggttgctcc agatgctgca
      181 cggtcttgtt gagctcgtaa ttggtggtcg aatccttgcg atcgctgacc ggcgtggtcg
      241 tcacaccggc cgtgccgctg gccgccgtga tcggtgcgga aacaggctgc ggcggctggt
      301 tcgacagcgc gcccggcacg cccgaggcgc cggcttgcga catctcggtg gcggtgctcg
      361 attgctggct gcggatcgcc gcctgctgcg ggctgccgtt cggaccgtag ttttccgagg
      421 tctgctcgct gtgggagaag tcgatatcgg cgctgacctg cgagtgcgcg ttgccggagc
      481 cgaacagggg ctccaggatc gcgtcgatgc gctgttgcgt gttgcgttcg atctgctgac
      541 ggtacttcag ttgcgaggcg tccagaccgc tgccggtgga cgactgcgtc agcagattgc
      601 cgtcctggtc gaggatggtc acgcccttca ccggcatgtc cgccaccgcc gacgccacca
      661 gatgggtgat cgccagcacc tggccttcgt cgagcgcgcg acccggatag aggttgacca
      721 gcacggaagc cgacggcgct tccttgtcgc gcacgaacac ggaaggtttc ggaatggcca
      781 gatgcacgcg cgctgacttc accgacgaaa tcgactcgat cgtgcgctcc agttcacctt
      841 ccagcgcgcg ctgataattg acctgctcgg cgaactggct aatgccgaat ttctggttgt
      901 ccatcagttc gaaaccgacc gagccgctct tgggcagccc ttgcgaagcc agacgcaggc
      961 gcatttcgtg cacctgctcg gccggcacga ggatcgcgcc accggagtcg gagaacttgt
     1021 agggaatgtt ggcggcctgc agcgcggtga tgatggcgcc gccgtcgcgg tccgacaggt
     1081 tgctgtagag caccttgtag tccggcgccc gcgaccacag gaacagaccc gcgacgatgg
     1141 cgaccagcaa cgcgaccgcg aaaatcagcg gcgcgcgcgg gttgccgcgc atttgcgcga
     1201 gccccgacag gcgctggccg aagttgccgc ccaggttagc cccgaggccg ccgagatcgg
     1261 ctgcgccgcc ggcctggccg gcgccggccg cggcgccgcc tggctgcgcg ccggccaggc
     1321 ccatgcgggc gtcggggttg atcaacgagt tggctgacga atccatgcgt cgggtatctc
     1381 cgggatgcct ttgcgtttct gtcgcttagc cgcaccgagg gtcggcgggc gcacggacag
     1441 agactttcac cattgaaatt atccgcagcg ccgctcaacc cgattgcttg aatagagccg
     1501 ggtttccccc ctagtttcgg ggctttcccg gatggtccac tgctatgctt tcgctcagat
     1561 cggtacggtt tctggcatgc cggtcgtcat tcctggagag aacatgactt tgcccgtgaa
     1621 cgcgctctcg tcggcgctgc aacagatgca gtcgatggcg gcgcaagcgg ccggcagcag
     1681 cacggcggcc ggcgtggccg acgcgtccgg cgcggcttcg gccggcggtt tcgcctcggc
     1741 gctgaaggcc tcgttggaca agatcagcag tgatcagacc aaggcggtcg gcgaatcgca
     1801 ggcgttcgaa ctcggcgcgt ccaacgtttc gctgaacgac gtgatggtgg acatgcagaa
     1861 ggccaacgtc ggtttccagt tcggcctgca ggtgcgcaat aaactcgttt cggcctatag
     1921 cgacatcatg cagatgtcgg tgtaactgcg attcaaagcg attcgggcgg tacggctgtt
     1981 ccgcccgccc gaacgcatgc gcccagttgc aacgccgtgc ggctgcacgg caggacagtc
     2041 cgcccggcgc gcccttgcgg cgcttcgtgg cagacggcct tgccgcgccc agcgcgatat
     2101 cgtccccgta atcctcctta catcggccta aagctttccc gctgcctatc cgataaccag
     2161 atacaggcag agatcggggt gcggcaatgc atccggatcg ggtaccacga ccaaacgtag
     2221 gacaagagag gaggagcgcc gatgctgtcg ccaggagact ctggagccaa tgcgtacgcg
     2281 cacgtcggcg tcgagacaga ggtgatgggt gcaagtccgc atcgtctgat cgtgatgttg
     2341 tatcagggag cccggcgagc catcgcgcaa gcgcgcacgc atattcagat gggcaatgtg
     2401 ccggctggag gagaggcgat cggcaaggcg attcagatcg tcgaaagcgg gctgcagcag
     2461 tcgctcaacg ttgaagcggg cggcgagatt gcagagcggt tgaacgcgct atatagctat
     2521 atagcgcgcc ggctgctcga agccaatata aaacagagcg aggcgatgct ggtcgaggtg
     2581 gacggcctgc tggcgacgct cgaagaggca tggagcggca ttgcccccga gatcgcgcgg
     2641 atggcagccc agccggccgc cgcggaaagc atgagatgac atcgaacgta gaatatttcg
     2701 cccgctacga ggcgatcgca gcgatttccc gccgcatgct gacggctgcc cggcgtgccc
     2761 tgtggagcga tctggcccac ttgcaggagg aatatcggaa tctggtggaa tcgttgagag
     2821 acgcggaaac cggcgtcaag ctaagcgatg cggagcgcat gcgcaaatac gcactgatct
     2881 gtcagattct ggccgacgac gctgcgatcc gcgatctggc gaatccgcgt atggccaatc
     2941 tgtcggcgct gttcgccgga cggccgactc gcgtgctcaa ggaactgtac ggggcgcgct
     3001 gagttcatcg gcgcagcgcg cgtttgcgcg agtctgcccg acagtatgtg agcgcgttgc
     3061 cgccaatgtg acgaaggaat cggtatgaac ggaatcgaca cggtcgtcag ttcgctgctc
     3121 gcgaaccggg tcgacagtct gctcaatctc gcgcccagca gtgtgaccgc ctcccagacc
     3181 ggtgcggcag gcgtcgatag cgcgcctatc accgtcacac ccctcacgcc acccgccacc
     3241 ccgccggctt ccgcgcaaac cgcgctgtcc gccgtcgcgc tgacgctcaa tgcgatcatc
     3301 aactcgggcg gcgaagcaac tccggcggtg ctcggccagg tgcccatctg gccggccgcg
     3361 cccgcgctcg atgtcgagcc cggcggcttg ccgctcttcg atacgccggc tgcttcatcc
     3421 acgaatccga atgccgccgc ggcgacggcc accgccaatg tggcggcggc tcaggtgccg
     3481 gtcgcggcgc tcgcggccgc actcgaacag acggtaggcg ttagcggcct gttctacgaa
     3541 tcccatctcg cacaatggct ggcggggcag cggccgcccg ccgcgctcgc tgacgaggcg
     3601 cagaacaagc tggtggcggc cgcgacgcaa ttgccgctcg attgggcgag cgacgccgat
     3661 caggcttcct cgtccaacgc ggcaccccgt cagggcacgg gcacgggcac aggccccaac
     3721 ggcccggcca acggcgcgcc ggacggcaac gcggcagccc gtgcgacgcc gtcgatccag
     3781 accgcgcagg ccgcgcgttt cgtagcgggc gaggtgttgg cgagttctct gtccgacctg
     3841 aacggcctgt cggcgcacgc gagcgtgcac agcgccgcgg cgcaactggc cgatgacggg
     3901 tcgtcgcaaa attcgcaatc gatggcggcc gcggttcatc ccgcgactgt tcccctggtg
     3961 cgtcagcaac tcgatctgct tgccaccggg cagtttcgct ggaccggcga agcatggccg
     4021 ggcgccaagc tcgactggac cattgagcag gaaggcgacg aatgggaccg cagcggcggc
     4081 ggcacggcaa gcgaggagga tcacccctgg cggacgcgcc tcacgctttc gctgccgacg
     4141 ctcggcaccg tcgatgcgga cctgacgctg accggctcgc ggctggtcgc gcgcgtgcag
     4201 gcgagtccgg ccggtgcggc gcggatggcg atgcaggggg agagcttccg gcaacgactc
     4261 gcggcggcgg gcatcgaact gaacggcctg acgattcgcg aaatcggcgg cggcgcgccg
     4321 gctaacgcgg cgggcgcggt gggggcagcg gcaaccgcgc aagcggcggc gtcggcgtat
     4381 gcgcgctcgg cggcgtcggc ttcgacggct gacaaggtgc cggcggggcg ccgcgatgcg
     4441 cgtgctggtc gcccgggcgg cgcgccgctc gacgatttcg actgggatat gtgatgagcc
     4501 gcagacatcg ccgcagcgcg gccgcgctcg tctacgacgc acaaggcagc gatcccgcac
     4561 cgcgcgtgat cgccaaaggc tacggcatgc tggccgagct gatcgtgcaa cgcgccaagg
     4621 aggctggcct gtatgtgcac gaggcgccgg aaatggtctc gttgctgatg caggtcgatc
     4681 tcgatacgcg gattccaccc gagctttatc aggtggtggc ggagttgctc gcctggctgc
     4741 atcggctgga aagcggtgcc gagcctgagc cggaaagcgc tgcggctggt gttcaggacg
     4801 tcatcgatgt ggttgcgacg gatataacgg acgtttcggg cggggcggtt gggcgttgag
     4861 tgctgcaagg gaccgtcgtg tcggctgctt gtcacgcggt cgccgtggcg tcgtgcggtt
     4921 gacccggttg acccggttga ccgtcccacc gttgcctact ccatgggtga tcgccctgcg
     4981 gttgcctgcc gcgcggatgg cgccccacgg atgcccgccg cacggatggc cgctccacga
     5041 atggccgccg catcaaaacc gcatcatcag cttcagcaac gcattcaccc gcttgccata
     5101 cggcggggaa atccacccac gcgcgttcag gcgcgcctgc gtcagcaccg gcttcatctt
     5161 cgagaaggtg acgaagcctt catagccgtg atacgccccc atcccgctcg ccccgacgcc
     5221 gccgaacggc agactttcgc acgccagatg catcagcgtt tcgttgatcg ccatgccgcc
     5281 cgcgatggtt tcgcgcgtga cgcgttcgat ggtggcggtg tcgtcagcgt agagatacag
     5341 cgcaagcgga cgcggacgtg cgttgatcca cgcgatcgcg tcgtcgagcg tgtcgtagga
     5401 gacgatcggc agcagcgggc cgaagatttc ctcctgcatc agcgcgcttt catccggcgc
     5461 attcgtgacg gcgatcagcg ggaagcgacg gcttgcctcg tccggcgtgc tgtcggtgag
     5521 catgtgcagt tgcgcgccgg cggcctgcgc ttcgtcggca agacgttgca gccgcgcgaa
     5581 gtgccgctcc gagatgatcg acgtgtaatc gaggttgtgc gcaaagccgg ggtacatctt
     5641 cgccatccgc gcgcgcgccc gtgcgatgaa agcctgctcc ttgccgcgcg gcactagcac
     5701 gtagtccggc gcgacacagg tctggcccgc gttgagcgtc ttgccggcga tgaggttatc
     5761 caccgcgttg tcgaagcgcg catgcagacc gatgatggcc ggcgatttgc cgccaagctc
     5821 aagcgtgagc ggcgtcagat gttcggcggc ggcgcgcatc acttcatggc cgaccctggt
     5881 cgagccggtg aacagcaggt ggtcgaacgg ttgcgcgctg aacgcggcgc ccaatgcggc
     5941 gtcgccgttc accaccgcga cgtgatcgcg cgcgaaggcc tggccgatca attgctcgaa
     6001 tagcgccgag gtgcgcggcg tcagctccga catcttgatg atcgcgcggt tgccggccgt
     6061 cagcgcgctg atcagcggac cggcggcgag cagcaccgga taattccacg gcacgatgat
     6121 gccgaccacg ccgagcggct gcggcacgac tttcgcgcgc gccggcatca gccatttgtt
     6181 ggtgctgcgg cgttgtgctt tcatccagcg tttgccgtgc cggagcgcgg cgtcgatctc
     6241 acccttgatc agcaggaatt cggcgagcaa cacttcctgt ttcgcgcgat tgccaaagtc
     6301 ggcgttcatc gcgtctgcga gtgcgtcgcg gttgtcgagc atgactttgc gcaacgcctt
     6361 tagatgcgcg gcgcggcttt cccacgacgg atacggcgcg cgcaaataag catggcgttg
     6421 atcgcgtagc agcgcttcga gcgcggcgac ctcgggtaga tcgttcttca tgtgggtcca
     6481 ctccctgatg gatatcggtg cgcgttgagt cgcgcttatc tgcttggttg tcctgtcttg
     6541 ctggcaacga tcttgcgaaa gcggtgtaaa agcgcgggcg agtgggtccg ctcaagagtg
     6601 gcagcgcggc atcgctgtcg atccgctcgc ttcagagcgg gacgcacacc gcgtcgattc
     6661 gctcacttcg cttcaaacgg gaaacgcccg ctcgatgatc gccgcgtggt cgaacgtgac
     6721 cggcagcgta ccgtacacgc ggccgctctc gccgcagccg tcggcgagcc gcgtcgcgat
     6781 gaaggcctcg gcgacttccg gcggcgcgtg cttcaccagc aaggtggctt gcgcgatcag
     6841 cacgatctgc tgcgcgatcc gccgcgcgga gctttcccgc gtgtcgggtg cgccgttcag
     6901 cgtggcggcg agcttgccga gcgcggcgct cacagccggg tgtccttgcg catccgcttg
     6961 ccacgcggcg aacaaggcct gcgcggcttc gggctcgcgc tccatcgcgc gcaacacgtc
     7021 gaggcacatg acattgcccg agccttccca gatcgaattc acgggcgcct cgcgatagaa
     7081 gcgcgccatt ggaccggtct cgacataacc gttgccgccc cacacttcca tcgcctcgcc
     7141 ggtgaactcg agcgtgcgct tgcagaccca gtacttcgac gccggggtga cgatccggcg
     7201 ccatgcgcgt tctgccggcg cggacgacga cgcatccgct gacgcttcga aagcgcgtgc
     7261 gagtcgcatc aacagaaccg tcgcggcttc cgattcgagc gccaggtcgg cgagcacatt
     7321 gcgcatcagc ggctgatcgg ccagatgccg gccgaaggcg ctgcggtgcc gtgcatgatg
     7381 aatcgcctgc accagcgccg cgcgcatcaa ggccgcgctg ccgatcacgc aatcgagcct
     7441 cgtgtagttc gccatttcga tgatggtcgg cacgccgcgg ccttcgtcgc cgatcatgat
     7501 cccgaacgcg tcgaggaact cgacctcgct gctggcattc gaccgattgc cgagcttgtc
     7561 tttcagacgc tggatctgca cggcgttctt gctgccgtcc ggtgcgaaac gcggcacgaa
     7621 gaagcatgag aggccttcgt gatcgtcggt acgggcgagc accagatgcg cgtcgcattg
     7681 cggcgcggag aaaaaccatt tgtgaccgac aaggcggtat tcggcgccgc ggccgctgcc
     7741 gcgtgtggca tacgcgcggg tctggttgct gcgcacgtcc gagccgcctt gtttctcggt
     7801 catccccatg ccgatcattg cggaggtttt ctgcgcgagc ggtaagtcgc gtggatcgtg
     7861 ttcgcgggta tagagcttgt cgcgcagtgt gtcgaacaac gcgggttcgc gttgcagcac
     7921 cgggatgctg gcgaaggtca tcgtcagcgg gcagagggaa ccggattcga gttgcgcgtg
     7981 caggaagtag ccggcgcaac gcgcgaccat tgcgccgcgc tgcggctccg aaaacggcaa
     8041 cgcgtgcagg ccttcgcggc gcaacagcga cagcagcgta tgccacgaag gatgaaactc
     8101 cagcgcgtcg atgcgttcgc cacgcgggct gtgcgtgaaa agttcgggcg tatggcggtt
     8161 cgccagttcg gcgagcgcga gcgtttccgg cgtggtgagc gccgcgccat gccgctgcag
     8221 gccttcgcga tgccacgcgg ccccttcgcg ttcgagggcg gcggacaacg ccacgtcgct
     8281 cgaaaacagg ttgtagtccg cgagcggcgg cgcctgattc gtcacgtcgt gggtgcggcc
     8341 gaagctgtgc gctttcatct gctgtctccg ttcaccggaa gcgcgccgtc gctgctcgac
     8401 ggtgtgctgt tttatcaccg cgtgtcatgc cgcacgctca gcggacggcg cccggcgcgc
     8461 cgcgtttgaa tgccttgcga agccgcccgg tcggcggatg cgcgtctttt atcatagggt
     8521 cgcagggacg ctttcgttta gaggaatcgc tctgacacga acggcgatgg cgatggcggg
     8581 aactgccggc gtcgccgctt gacggcgcat cagcgcttga cagcgagcgt cgcccgagtc
     8641 gggtggcgcc ttacgttgct cttcgcgcgt tcagcgtgcg agcccgaggc acgcatgtgt
     8701 gcaaaatcca ctagtcttcg cttgcctcac tccggtcacg gcggcgcatc aatcttccta
     8761 atctgggtcg caaatataag aatatttagg cgaacgtcgg ttaattcgtc ataaattgtg
     8821 ccgttccgtt tatgagattt ccccaaacct ataatcgcgc cgcgaaaaag agcgcgtcag
     8881 gtgacgcgtt cgggaaatca aggaagcgaa gcgatgagta tcaatgtgat tcagctgatt
     8941 cagtccgcgt tgacggacag cgtcgtgcga cagctggcgg cccgcttcgg gctgccgccg
     9001 gacgcaaccc agaaggtgct ggcaacaacc ggcccggcgt tggtcgccgg cctgatgcag
     9061 aagggcgcga cgctcgacgg cgcgcgctcg ctgttcgcca cgatcctcgc gcccgaggtg
     9121 aacgggcaca tcgccgagca gttgccgcaa ctgctcggca ccaccacggg cgtgagccag
     9181 ttggaaggcg tggggcggca gttgctcgag cgcgcgctcg atcgccgcgt cgatacgctc
     9241 agcgacgaag tcgcgaccca aaccggcgtg ccggcgcatg ccacgcacgc catgacgggt
     9301 atcgtcggcg caacgctgct gggcctgttg aaacggcatt ttctggaagg tcagggcaac
     9361 gtcggccact tgccgacact gctgggccat caactgccgg ccgttgcacc gcacctgaac
     9421 gaccgtttga tgacgggcct cggtctgagc ggactcggcg cattcaccgg caatattctg
     9481 tcgcagttga aagcggtctc ggcgcatatc gaccatccga cgcctgccgc caagccggta
     9541 gccgaagccc tgtcgagcat ccgtgtgcct gccgacgcag tggtgcgcga agagcgccgt
     9601 ccgcgcaagt ggctgtggtg gctgctcgcg gcggtcgccg ccgtgctggc tttcctgttc
     9661 ctgcgcggtt gccatagcga gcaatcggcg gaacgcagca acgcggaagc cgcggtgact
     9721 gcgcaacccg cgtccgcacc ggtggaggcg tcggccgccg cggctgcaac acccgcggct
     9781 agcgatgtcg cggcttcgac gcccgctgcc gccgctgcag caagcgccgc cagtgacgcg
     9841 cagctacccg cagccgcacc cgcgccgacc agggacagcg tgctgagcct caaggtcgac
     9901 gccggcggca agccgacgct caccgcgacg ctcggcagcg aagccgaaaa gacccagttg
     9961 atcgaggcgc tgaccaggcg cttcggcgca gaccggttcg tcgcgaacgt cacgatcgat
    10021 caggacacca agccggccga ctggctcgct cacctggacg gcctgctgcc gttgatggca
    10081 ctgccgggtg cggaagtgaa agtggatggc gcccacatcg aattgagcgg cagcgcggcg
    10141 aacgccaggc tcggctggct cgacaagctg aagtcgctgt tcggcgcgtc gtaccagatc
    10201 ggttcgttca acgtcgaaca ggcggttgcc aacgcaaccg agaccttccg cggcgcgatc
    10261 aagggcctgc tcacgccgga cagcccgtgc gcggcagcgg acgtggtcaa ggtgctgaat
    10321 ctgcaggtga tcaacttcac gagcgccagc gcccgcgtgc cggcctcggc gattgaagac
    10381 ctgaaccagt cggcgcaagt gctgaacgcc tgtgcgcgca acggcaagac ggccaagctc
    10441 gaagtggccg gctattcgga caacgtcggc ggcgcacaag ccaatctgca attgtcgaag
    10501 aagcgcgctg aagccgttcg cgcgtatctc gtgaagacgg gggtgccggc ggattcgcta
    10561 gtcgcgcaag gttatggcga cgcgcaaccg gtcgcgagca acgacacggc gagcggccgc
    10621 tttgcgaacc gccgcatcga gttcgtcgcg caatagtagt gacggcgtcg tcaaggtaaa
    10681 cctcgcagta atcccttggg gcgagccaaa aaaaaacatg ccgcccggtt cgcgccgggc
    10741 ggcatgttgt catttggccg gccgcgtttg cggtggccgc atttgcgcta gtcgcattcg
    10801 cgctggcccc atttacgacg gtgtctggct cggtgcggtg atgccggaga taccgatacc
    10861 cggccccacc atgaaatcgt caccggcctg gcccggcagc gtctcgcggg ccacctccag
    10921 ccacgcgcgc gccgcgtgcg acagatagcc gttacgacgc cagccgatcg ccatttccca
    10981 cggaatctcc ggttccacca ccgggcggca ggtgaactgc gccgcgtcga gccgctggca
    11041 atacggcgcg ggcagcagcg cgatgccgac gccggccagc accagcgccg ccatgaaatc
    11101 ccaatgcccg ctgcgcccga cgatggtcgg cgcaaacccg gcggtgcggc acgcattcac
    11161 cacgacatcg ttgagcgcga ggctctcgcc gtagaacacg aacggctcgt gggcgagctc
    11221 ggcgagcggc acttcgtgca ggtcgtccca gcgcgaaccc gtgcgcgcga cgagccacag
    11281 caattgccgc gtcatcggca gcacctcgat gttttccggg tcgaccggtt gcagcacgcc
    11341 gcctagctcc agctcgccgt ggatcagcgc ggcttcgatc gcgcgcgcgc cctgctcgaa
    11401 cagcttcagt tcgattttcg ggtagcgctg gcggaacgcg gcaatggccg gcgtgaagag
    11461 cgagccgccc attggcggaa tgccgatggt cagctcgccg cggccgagcg tgccgagatc
    11521 gttcagttcg gcctgcaact gcgcgtgcgc cgccagcacg tcctgtccgc gctggtagac
    11581 gatccggccg gcgtcggtga gcaccatctg acgtccgtcg cgcaacagca gcggcgaacc
    11641 gatttcgtct tccagcgact tgaccatttt gctgatggtc ggctgcgtga cgaacatctg
    11701 ctcggcggca acggtgaagc tctgttgacg gactacttcg acgaaatacc gcagcgcgcg
    11761 tagttccacg atctcggctc cagaattcca atttggaatg ttccggacga ttgtaagtca
    11821 tcgtatttat attgcggccc aacttgatgg cgcctgttcg tagcggcgcc gttcaggcgc
    11881 ggcgcgtagt tcggtgcaat cgcgcagatc gcttttccag gatggacgcc gccggaaacg
    11941 ggctaccatc ggcggttctc taccgttcgg cccgcagcgc ccgtttcaat tcatgacgcc
    12001 catctcgatc atcatcccct gctacaacgg cgcggcgacg ctcgcccgcg cactccaaag
    12061 ttgcctgatt caacccgaag ctgcgcagat catcgtggtg gacgacggtt cgaccgatgc
    12121 gtccgccgag ctcgttgcct attgcggaag cctcgacgaa cgcgtgggcc tgttgcgcat
    12181 gccggtcaac ggcggcgcgg cgcgagcgcg caactggggc gcgatgcatg cgtcgcacag
    12241 cctgctggcc ttcctcgatg ccgacgacga atacctgccc ggcgccctgg ccgccgccag
    12301 cgcgtttctc gtgcagaacc ccagggaagt gtccgtgcgg ctcgacgtgg aatacacgga
    12361 tttcccggcc gacatcgtca gccacgccca tttcgacaag catgcggcga cgctcagcaa
    12421 tacggtgccg agcagcctga tcatccgccg cgcggtgtat gccgcgctcg gcggcttccc
    12481 gatggatgac gcgtttcgcc gcatcggcgg agaagacggg gcgttttcct gggcgctgcg
    12541 cgagattttc ggcaaccggc gcctcgacga tgtgaagcgc gtgcgcatgc attaccacgc
    12601 cggcattcac gccgaacgct atttccgcat cgcgctcggc atgcggaagc cggatcccgc
    12661 cgatctggat gacgcatttc gtttttccag gcagttcgtc gaaaccgcgc gcgcgggtat
    12721 cgagcaattg cgcgcggcaa gcgtcgctgg cgaagccacc gcggctatga ttccgaatca
    12781 ggccgcgtag gttccagaca ccgaagcagc ccgacagcag gacaaggtgc gccgcgcccg
    12841 cactagactc ctgccaaagc cccaaagccc caaagcccca aagccccaaa gccccaaagc
    12901 cccaaagacc caaagcccca aagcccc
//
LOCUS       DATASH010000923         3306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3123352,
            whole genome shotgun sequence.
ACCESSION   DATASH010000923 DATASH010000000
VERSION     DATASH010000923.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3123352"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /gene="acpS"
                     /locus_tag="VHV99_24670"
     CDS             complement(<1..400)
                     /gene="acpS"
                     /locus_tag="VHV99_24670"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEX3638615.1"
                     /translation="MILGIGSDLVDVGRIEKVIERHGERFLARIFTPTERARAERRAD
                     RAATYAKRFAAKEACAKALGTGLRKGVFWRDMGVVNLPSGRPTLKLSGGALARLKAIT
                     PEGCEAQIDLTITDEGSMAQAFVVISAVRRA"
     gene            1038..1931
                     /locus_tag="VHV99_24675"
     CDS             1038..1931
                     /locus_tag="VHV99_24675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEX3638616.1"
                     /translation="MNDARTDDDEQLSPRVAALESTVTSLAADVPRTDDVAERLAALE
                     HAVADHGQQLQVRAVMDWIERATLRSAPLVSVVLPTRDRRDLLPRAIESVQKQSYGNW
                     ELLIVDDGSVDGTAEFLAGLAGDRVRCFRHHGAGACAARNVALAQVRGDLIAYIDDDN
                     MMHPAWLKAIVWGFEQRPEANVLYGGYVVDDAWRINKKRSGDLPRLFFLPYDHHQVAR
                     RSIADMGCIAHRAGLPEARFDESLREMGDWDLFLRLTRTAPPLALPAIACFYTTDAPN
                     RLSHGPTFAADVAAVRAKNRR"
     gene            complement(1874..2701)
                     /locus_tag="VHV99_24680"
     CDS             complement(1874..2701)
                     /locus_tag="VHV99_24680"
                     /inference="COORDINATES: protein motif:HMM:NF016459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638617.1"
                     /translation="MGQLFQSFWWGGPLSPYEWLSLKSFIDFGHDFDLYSFDSHIAVP
                     AQVRVRDAAEIAGRAEFFVYESGFGKGSPAAFANLFRYKLLAERGGWWVDTDVVCLSD
                     AIPDYPQFVARQVDGLINNAVLRFERRHPVMLRCIELAEEKGRSVRWGDTGPQLLTRI
                     MQELGETQGIFDPSVCYPVDHLHALDVLRPSALARVTARTKGSQLLHLWNQLLKQAGV
                     RKTHLPPRGSLLRALVERHQIGGWDGEYDEAEVSAAVERYRRFLARTAATSAANVGP"
     gene            2996..>3306
                     /locus_tag="VHV99_24685"
     CDS             2996..>3306
                     /locus_tag="VHV99_24685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HEX3638618.1"
                     /translation="MRIAKGIALKLASLLLFGVMSALIRGFGDTIPVGQVVFFRSAFA
                     IVPVVILYAARGELVTTLRTEHPFRHIARGIISVLGMFLSFAALARLPLVDATAISFA
                     AP"
BASE COUNT          583 a         1109 c         1022 g          592 t
ORIGIN      
        1 ccgcgcgccg caccgccgat atgacgacga aggcctgggc catcgaacct tcgtcggtaa
       61 tggtcaggtc gatttgcgct tcgcacccct ccggcgtgat ggctttaagc cttgcgagcg
      121 ctccgcccga aagcttgagc gtcggccggc ccgacggcag gttgacgacg cccatgtcgc
      181 gccagaacac gcccttgcgc aaacccgtgc ccagcgcctt cgcgcacgcc tccttcgcgg
      241 cgaagcgctt ggcgtaggtc gccgcgcgat cggcgcgccg ttcggcgcgc gcgcgctccg
      301 tgggcgtgaa gatgcgcgcg agaaaccgct ccccatggcg ttcgatgact ttctcgatcc
      361 gcccgacgtc caccaggtcg gagccgatgc cgagaatcat cgccagtgtc cctgccgcac
      421 gttccgaagc aaacttcgga atcgaaggac actagcaaat ctatgattct agtgccgctt
      481 tgaacccgaa gttcgcaatc ggactcgccg caattgtagt gcgaacttcg ggttcgcggc
      541 actagagcgt gatgactttt cttcgaatcg tcatcacgct ctaacttctt cttggcgcat
      601 gatgttttcc gaaaaccggt acccactttt cggcatcatg cgctagcatg gcgtttcgga
      661 cgatacacgg gacggcgccg aagcgctacc tgctcgctct gggcttgaaa aaggcgcgcc
      721 ggaccttgct ctcgaatgac ggcgcggcca caagcccgac ggaggtggcc acccgcttcg
      781 gtttccgtgc gcttggccgt cgcgtggcac gcaggcatga cggcactccc gcgaactacc
      841 gtagccgggg aagcccttcc gccacacgac gccggtaatg ctagcttagt tggcgatcct
      901 gccctatggg acaattgaac gcgcggcgcg gaatgcgtac ctgaattttg ccgcgacgtt
      961 ccggcctgcc ggcctgtttc gagtgagcat cctcgcgtcg cagcgagggg tccgcatgtc
     1021 ttgaggagcg aagccacgtg aatgacgcac ggacggatga cgacgaacaa ttgtcgccgc
     1081 gcgtcgccgc gctcgagagc acggtgacga gcctcgcagc cgatgtgccc cggaccgacg
     1141 acgtagccga gcggctcgcc gcgctcgaac atgcggtcgc cgatcacggg caacaactgc
     1201 aggtgcgcgc cgtcatggat tggatcgagc gcgccacgct gcggagtgcg cccctggtgt
     1261 ctgtggtgct gcccacgcgc gatcggcgcg atcttctccc gcgcgcgatc gagtcggtgc
     1321 aaaagcaaag ctacggcaac tgggagctgc tcatcgtcga cgacggcagc gtcgacggca
     1381 ccgccgaatt tcttgccggc cttgccggcg accgtgtgcg ctgtttccgt caccacggcg
     1441 cgggcgcgtg cgcggcgcgc aacgtcgcgc tcgcccaggt gcgcggcgat ctgatcgcct
     1501 acatcgacga cgacaacatg atgcacccgg cctggctcaa ggcaatcgtg tggggcttcg
     1561 agcagcggcc ggaggcgaat gtgctctacg gcgggtacgt ggtggacgac gcgtggcgca
     1621 tcaacaagaa gagatcaggc gacctgccgc gccttttttt cctgccctac gaccatcatc
     1681 aggtcgcccg caggagcatc gccgacatgg ggtgcatcgc ccatcgcgcc ggcctgccgg
     1741 aagcccgctt tgatgaatcg ctgcgcgaga tgggcgactg ggacctgttc ttgcggctga
     1801 cccgcacggc gccgcccttg gcgctgcccg cgattgcctg cttctacacg accgacgcgc
     1861 ccaaccggct cagtcacggg ccgacgttcg ccgcggacgt ggcggcggtc cgcgccaaga
     1921 accggcgata acgttccact gccgccgaca cttccgcttc gtcgtattcg ccgtcccatc
     1981 ctccgatctg atgtcgttcg accagggcgc gaagcagcga gccgcgcggc ggcagatgcg
     2041 tcttgcgcac gccggcttgc ttcagcagct ggttccacag gtgcagaagc tgcgacccct
     2101 tcgttcgggc ggtgacgcgt gcgagcgccg aggggcgcag gacgtcgagg gcgtgcaagt
     2161 gatcgacggg ataacagacg gacggatcga aaataccctg cgtttcgccc aattcctgca
     2221 tgatccgggt cagcagctgc ggaccggtgt cgccccagcg gaccgaccgc cccttttcct
     2281 ccgccagctc gatgcacctc agcatgacgg gatggcggcg ctcgaagcgc agcaccgcgt
     2341 tgttgatcag tccgtcgacc tggcgcgcca cgaattgggg ataatcagga atggcatcgg
     2401 acaggcaaac aacgtcggtg tcgacccacc agccgccgcg ctcggccagc agcttgtagc
     2461 gaaacaggtt ggcaaacgcg gccggggacc ccttcccgaa accgctttca tagacgaaga
     2521 actccgcccg gccggcgatt tccgccgcgt cgcgcacgcg gacctgcgcc ggaaccgcaa
     2581 tgtgagagtc gaacgaatac aggtcgaagt cgtgtccgaa atcgataaat gatttcaggc
     2641 tcaaccattc gtacggagac aggggcccgc cccaccaaaa cgattgaaaa agctggccca
     2701 tttcgggatc ccgatcggtt gcgcaagcct cgtctaggta ccatatcaca ccgacgcgat
     2761 ctcatcttcg ctcacgattc cgtgagacac cggtgtcccg tccagcaggt ccccacaccg
     2821 ataaagaaat aacacgattt tgccggctgt tgggcgcatg acacacgagc ctttgccata
     2881 cgtttccgac cgacgacgca tgcacacgca accgacttcc ggcgaagctg atggcgcgat
     2941 cgattcgcat gccgaaatca ctcccgccct ccgggcggcg cctgggcgcc gctccatgcg
     3001 tatcgccaag ggaattgcgc tcaagctcgc atcgctgctg ctgttcggcg tgatgtcggc
     3061 gctcattcgc ggcttcggcg acacgattcc ggtcgggcag gtggtgttct tccgcagcgc
     3121 cttcgcgatc gtgccggtgg tgatcctcta cgcggcgcgg ggcgaactcg tgaccacgct
     3181 gcgcaccgaa catccgttca ggcacatcgc acgcggcatc atcagcgtgt taggcatgtt
     3241 cctgagcttt gcggcgctgg cgcgcttgcc gctcgtcgac gccaccgcca tttccttcgc
     3301 cgcgcc
//
LOCUS       DATASH010000924        11095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6484961,
            whole genome shotgun sequence.
ACCESSION   DATASH010000924 DATASH010000000
VERSION     DATASH010000924.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 11095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11095
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6484961"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /gene="adh"
                     /locus_tag="VHV99_24690"
     CDS             complement(<1..817)
                     /gene="adh"
                     /locus_tag="VHV99_24690"
                     /EC_number="1.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEX3638619.1"
                     /translation="MNHAEMQFLTTEFPYKKQYGNFIGGEWVKPVGGEYFDNVSPITG
                     EPFTSIPRSREADIELALDAAHRAKAAWGKTSTTDRANILNRIADRMEANLQRIAVAE
                     TIDNGKPLRETMAADIPLAIDHFRYFAGAVRAQEGSLSEIDHDTVAYHFHEPLGVVGQ
                     IIPWNFPILMAVWKLAPALAAGNCVVLKPAEQTPASILVLLELIHDLLPPGVLNVVNG
                     FGLEAGKPLASSKRIAKIAFTGETTTGRLIMQYASQNIIPVTLELGGKSPNIFF"
     gene            1144..2217
                     /locus_tag="VHV99_24695"
     CDS             1144..2217
                     /locus_tag="VHV99_24695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3638620.1"
                     /translation="MTIESRSDNQITPTRPTHAQQPSFCVQTRVAYDADEQARNLHGW
                     NQTYDQLTAGRFVGGLTELCLDHMQVFVETTSHTLRQTCEVQEDVYWFGIPACPKGSG
                     RIDAQVIAGDALAFRPGGIEFELLTSAGYEIFGVVVKGEVLRRYAAEVERVGLADHLP
                     NTQVVSIGMARKARLCASLRQLLDDGAASGAPLSSFARNNLQASVLASLFDVGALPAA
                     GQVEMPARPRRQSIVSGAREYVLANRDRAINVPELCERLHVSRRTLQYCFQDVLGMAP
                     ATYLRAIRLNGARRDLCNASRETRSVQDVAAAWGFWHLSQFATDYRKLFGMRPSDTLK
                     AAAATRQATGDRSFAHQDHAAHC"
     gene            complement(2371..4854)
                     /locus_tag="VHV99_24700"
     CDS             complement(2371..4854)
                     /locus_tag="VHV99_24700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX3638621.1"
                     /translation="MPHANHAEADRPEQAKACAHGHEGHTHEHADDDAQCHGPSKSGG
                     PAHAAHAQERDHGHAHEHGECCNHGHQAHANESAHAHSHASGDSCGHGHADTDCCAAM
                     PVMPRPIKLPQSEVVGSDVRTAIRIMQMDCPTEEALIRKKFSRMPYVRSMDFNLMQRV
                     LTVVHAPDALDSILAAIRSLDFTPELADAVSGAASTRAPSAPPKPWWPLALAGIAAAA
                     SEAASWLGAPVWLAAGLAILAIASCGLTTYKKGWLAIRNGNLNINALMSIAVTGALIL
                     QQWPEAAMVMVLFTIAELIEAKSLDRARNAIQGLMQLTPEQASVRQADGSWRPTDLKA
                     IALGAVVRVKPGERIALDGEIVAGRSSLDQAPITGESLPVDKIVGDAVFAGTINQAGS
                     FDYRVTAAASNTTLARIIHAVEEAQGTKAPTQRFVDQFARVYTPIVFAAALAVAVLPP
                     LLFGGQWHEWVYKALVMLVIACPCALVISTPVTIVSGLAAAARKGILIKGGTYLEQGR
                     KLSWLALDKTGTITHGKPVQTEFEMLAEVEIDVDVDVDVVRCRTLAASLAGRSDHPVS
                     MAIAAAAKSAGVVSATVDAFEALAGRGVRGEIDGLPYWLGNHRLVEELGRCSASLETR
                     LDALERQGKTVVMLMDAERVLALFAVADTVKDTSRAAIAELQRLGVRAAMLTGDNPHT
                     AAAIAQQVGIDEARGNQLPEDKLNAVAQWSQEGATVGMVGDGINDAPALARADIGFAM
                     GAMGTDTAIETADVALMDDDLRKIPLFIRLSKATHSVLVQNITLALGIKSVFLVLTVM
                     GLGTMWMAVFADVGASLLVVGNGLRLLRK"
     gene            4935..5366
                     /gene="cadR"
                     /locus_tag="VHV99_24705"
     CDS             4935..5366
                     /gene="cadR"
                     /locus_tag="VHV99_24705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cd(II)/Pb(II)-responsive transcriptional
                     regulator"
                     /protein_id="HEX3638622.1"
                     /translation="MKIGELAKIAHCTTETIRFYEKEGLLPEAERTEANYRSYTARHV
                     ERLRFIRNCRALDMTHDEIRELLRLTDAPADGCGGINALIDEHIAHVDARIEELKQLK
                     VQLTTLREQCHGEQAVEDCGIVQGLTEMDVSATRARHTHLG"
     gene            5537..5737
                     /locus_tag="VHV99_24710"
     CDS             5537..5737
                     /locus_tag="VHV99_24710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638623.1"
                     /translation="MFTCRNQPCGEQWEMSDVVIKNEGQGLLFRCPMCGARNYVERFD
                     GEDGSVLYEQIEGRPATGPMAE"
     gene            5781..7184
                     /gene="dbpA"
                     /locus_tag="VHV99_24715"
     CDS             5781..7184
                     /gene="dbpA"
                     /locus_tag="VHV99_24715"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HEX3638624.1"
                     /translation="MNSPIQAGAPFSQLQLPPATLANLTQLGYVEMTPIQAASLPIAL
                     AGNDLIAQAKTGSGKTAAFSLALLNRLDVRNFAVQAMVLCPTRELADQVTQEIRRLAR
                     AEENIKVLTLCGGTPMRPQTASLEHGAHIVVGTPGRIMDHLERGSLPLQSLNTLVLDE
                     ADRMLDMGFFDDIATVVKQCPKERQTLLFSATYPEGIVKLSQQFLRNPKEVKLAERHD
                     NTKIRQRFYEVTEDQRLHAVGLLLNHYRPVSTLAFCNTKQQCRDLLDVLRAQGFHALA
                     LHGELDQRERDQVLIQFANRSCSVLVATDVASRGLDIAELEAVINVDVTPDPEVHVHR
                     IGRTGRADQEGWALSLASMNEMGRVGSLEQAQKREVEWHKLADLKPASNERLLPPMET
                     LQILGGRKEKIRPGDVLGALTGEAGFAGSQIGKINVTEMSTYVAVERSVARDALRKLS
                     AGKVKGKKVKVRMMDDA"
     gene            7682..8836
                     /locus_tag="VHV99_24720"
     CDS             7682..8836
                     /locus_tag="VHV99_24720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012327613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX3638625.1"
                     /translation="MQSAMQARSKLPDVGTTIFTVIGQLAAQHDALNLSQGAPNFAPD
                     AKLIDGVAQAMRAGHNQYAPMAGIATLREALADKVETLYGVRYDPSSEVTVIASASEG
                     LYSTISALVHPGDEVIYFEPSFDSYGPIVRLQGATPIPIKLSLSDFRVNWDEVAAAIT
                     PKTRMIIINTPHNPTATVFSEADIARLKAVTRNTDIVILADEVYEHVVFDGAKHQSMA
                     CDAELAERSVIVSSFGKSYHVTGWRVGYCLAPAALMDEIRKVHQFMVFSADTPMQYAF
                     VDALANRESYLGLSAFYQKKRDLLTHALRDSRFELLPSEGSFFMLARFRGFSEEGDSD
                     FVLRLIRDARVATIPLSAFYTDGTDSGLIRLSFSKDDATLIEGARRLCEI"
     gene            9031..9825
                     /locus_tag="VHV99_24725"
     CDS             9031..9825
                     /locus_tag="VHV99_24725"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3638626.1"
                     /translation="MTLLKPLLALACAFTSIAASSAAFAADTSTLRFGLEAQYPPFES
                     KGPNGELQGLDIDVGNAVCVAAHMTCKWVETSFDGLIPALQGRKFDAINSAMNATEQR
                     RRAIDFTTVVYRVPSQLIAKRDSGLLPTPASLKGKRVGVLQASIQETFAKTHWEPAGV
                     AIVPYQDQNQVYADLVAGRLDATLVLAPAGQTGFLSKPSGEGYAFVGQPVRDDKILGS
                     GIAYGIRKGDTALRDQLNAAIAKVQADGTVKTLAAKYLGNIDVSAK"
     gene            complement(9897..10643)
                     /locus_tag="VHV99_24730"
     CDS             complement(9897..10643)
                     /locus_tag="VHV99_24730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011886133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HEX3638627.1"
                     /translation="MSKSKLNPADLASPLSEEEFDELDEFLISDLTSEQTLTIMGLDG
                     YLTAMAIGPTTVSPGYWLPGVWGPSEDDTPAFESAGQAQHILGLILRYMNGIIDDVEH
                     DPDGFEPVFGVHRIDDSGSEYIDGEAWAMGFMQGLLLVEQDWQALFDSEQGREWLEPI
                     RLMGGLDLTLEDEELIGTPQLDEALTKQIPASVAAIYRFWLPHRQAVYERTLAATVQR
                     TEPKVGRNDPCPCGSGKKFKKCCGAAATLH"
     gene            complement(10777..10852)
                     /locus_tag="VHV99_24735"
     tRNA            complement(10777..10852)
                     /locus_tag="VHV99_24735"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10817..10819),aa:Lys,seq:ctt)
BASE COUNT         2105 a         3398 c         3491 g         2101 t
ORIGIN      
        1 cgaagaaaat gttcgggctc ttgccgccga gttcgagcgt cacgggaatg atgttctggc
       61 tcgcgtactg catgatcaaa cggcccgtgg tcgtctcgcc ggtgaaagcg atcttcgcga
      121 tgcgtttgct cgatgcgagc ggtttacccg cttcgaggcc gaaaccgttc accacgttga
      181 gcacgcccgg cggcagcaaa tcgtggatca gttcgagcag cacgagaatc gacgcaggcg
      241 tttgctcggc cggcttcagc acgacgcaat tgccggcagc caatgcaggc gcgagcttcc
      301 acactgccat caggatcggg aaattccacg gaatgatctg gccgaccacg ccgagtggtt
      361 cgtgaaagtg ataagcaacg gtgtcgtgat cgatctcgga gagcgagcct tcctgcgcgc
      421 gcacggcgcc ggcgaaatag cggaaatgat cgatggccag cgggatgtcg gcggccattg
      481 tttcgcgcag cggcttgccg ttgtcgattg tttcggccac ggcgatgcgt tgcagattcg
      541 cttccatccg gtcggcgata cggttcagga tattcgcgcg atcggtggtc gacgtcttgc
      601 cccacgctgc tttcgcacga tgcgcggcgt cgagcgcgag ttcgatatcg gcttcgcgtg
      661 agcgcgggat cgacgtgaac ggctcaccgg tgatcggcga cacgttgtcg aaatactcgc
      721 cgcccacggg cttcacccat tcgccgccga taaaatttcc atactgcttt ttgtacggga
      781 attcggtggt cagaaactgc atctctgcgt gattcatctg tgtgctcctc acatgttgaa
      841 tgggctatat tcggcgcgat atttcgcttg gccgcgaatg gctacagcca gaactgtgcc
      901 aatgcaccgc gctggtgagc cgtgcacgtg cagcaggcac gtctgcaaag tggggcgtaa
      961 agcgctgttc aaccggtcga gagatgaacg cgtcattttt gatcagtgtg cgcagtgctg
     1021 ttcaggaatt gagcgggacg tgagcacctg gcgttccggt ccaagccaac gaggcaagtg
     1081 gcggctgcgt tcggcatggg aattgccttc ctttgcactt caatacttca ttcgcggatc
     1141 ggtatgacga ttgaatcccg cagcgacaac cagataacac cgacgcggcc aacgcatgcg
     1201 caacagccga gcttttgcgt gcagacccgc gtcgcctacg acgccgacga gcaggcgcgc
     1261 aaccttcacg gatggaatca gacctacgat caactgaccg caggccgctt cgtcggcggt
     1321 ttgacggagt tgtgtctcga tcatatgcag gtgttcgtcg aaacgaccag ccatacgctg
     1381 cggcaaacct gcgaagtgca ggaggacgtc tactggttcg gcattccggc ttgccccaag
     1441 ggttccggtc gtatcgacgc gcaggtgatc gcgggcgatg cgctcgcatt ccgtccgggc
     1501 gggatcgaat tcgaattgct gacctcggcg ggctacgaaa ttttcggcgt ggtggtgaag
     1561 ggtgaagtgc tgcggcggta tgcggccgaa gtggagcgag tgggtcttgc cgaccatctg
     1621 ccgaacacgc aagtcgtgtc gatcggcatg gctcgcaagg cgcgtttgtg cgcgtcgctg
     1681 cgccagcttc tcgatgacgg cgcggcgagc ggcgcgccgc tgtcctcgtt cgcacgcaat
     1741 aatttgcagg cgtcggtgtt ggcgtcgctg ttcgatgtgg gcgcgttgcc ggccgccggg
     1801 caggttgaaa tgcccgcgcg tccccggcgt cagtcgatcg tgtcgggggc gcgcgagtat
     1861 gtgctggcta accgggaccg cgcgatcaat gtgccggaat tgtgcgagcg cttgcatgta
     1921 agccgtcgca cgttgcaata ctgctttcag gacgtgctcg gtatggcgcc cgccacgtat
     1981 ctgcgcgcga tccggctgaa cggcgcgcga cgcgatctgt gcaatgcgtc gcgtgaaacg
     2041 cgctcggttc aagacgtcgc ggccgcgtgg ggcttctggc atctgagcca gtttgcgact
     2101 gactaccgga agctgttcgg catgcggcct tcggatactt taaaggccgc agccgccact
     2161 cgccaggcga cgggagatcg ttcgttcgct caccaggatc acgctgcgca ttgctgataa
     2221 atcgcacgcg gctggcagcg cgacgcgacc atgcttggca ggcaacagcc aacagccaac
     2281 agccaacagc caacagacag cagacagcag acagcaggca gcaggcagca ggctccgcga
     2341 atgcgtggca tgacacggac ccgcgcacac ttatttgcgc aacagccgca aaccattccc
     2401 caccaccagc aggctcgcgc ccacatcggc gaacacggcc atccacatcg tgcccagacc
     2461 catcacggtg agcacgagga agacgctctt gatgcccagc gccaacgtga tgttctgcac
     2521 cagcaccgaa tgggtcgcct tcgacagacg gatgaatagc ggaatcttgc gcagatcgtc
     2581 gtccatcaag gcgacgtcgg cggtttcgat cgcggtgtcg gtgcccatcg cgcccatcgc
     2641 gaagccgatg tccgcgcgcg ccaaggcggg ggcgtcgttg atgccgtcgc cgaccatgcc
     2701 gactgtcgcg ccttcctgcg accactgcgc caccgcgttc agcttgtctt cgggcaactg
     2761 attgccgcgt gcttcatcga tgccgacctg ctgcgcgatg gcggcggccg tgtgcgggtt
     2821 gtcgccggtg agcatggcgg cacgcacgcc gagacgctgc aattcggcga tggcggcgcg
     2881 gctcgtgtcc ttcaccgtat cggcgacggc gaacagcgcg agcacgcgtt cggcatccat
     2941 cagcatcacg acggttttgc cttgccgctc gagcgcatcc agccgcgttt cgagcgacgc
     3001 tgaacaacgc ccgagctcct cgaccagccg atgattgccg agccaatacg gcaagccgtc
     3061 gatttcgccg cgcacgccgc ggcctgccaa ggcctcgaac gcatcgacgg tggcactgac
     3121 gacgcctgcg cttttcgccg cagccgcaat cgccatcgac accggatgat ccgagcgacc
     3181 cgcgaggctt gccgccagtg tccggcaacg aacgacatcg acatcgacat cgacgtcgat
     3241 ttcgacctcg gccagcatct cgaactcggt ttgcaccggc ttgccgtgcg tgatcgtgcc
     3301 ggttttatcg agcgcgagcc agctgagctt gcggccctgt tcgaggtagg tgccgccttt
     3361 gatcaggatg cccttgcggg cggcggcagc gaggccgctg acgatggtca ccggcgtcga
     3421 gatcaccagc gcgcacggac aggcgatcac gagcatcacc agcgccttgt agacccattc
     3481 gtgccactgt ccgccgaaca gcaacggcgg caacaccgcg acggcgagtg cggcagcgaa
     3541 cacgatcggc gtgtagacgc gcgcgaactg atcgacgaaa cgctgagtcg gcgctttggt
     3601 gccttgcgct tcctcgaccg cgtgaatgat gcgcgcgagc gtcgtgttgc tggctgcggc
     3661 cgtgacacga tagtcgaacg agccggcctg gttgatcgtg ccggcgaaca ccgcgtcgcc
     3721 gacgatcttg tcgaccggca ggctttcgcc ggtgatgggc gcctgatcga ggctcgagcg
     3781 gcccgcgacg atttctccgt cgagcgcgat ccgttcgccc ggcttcacgc gcaccaccgc
     3841 gccgagcgcg atcgccttga gatccgtagg ccgccagctg ccgtcggctt gccgcacgct
     3901 ggcttgttcg ggcgtgagtt gcatcaaccc ttgaatggca ttgcgggcgc ggtcgagcga
     3961 cttcgcttcg atcagttcgg caatcgtgaa gagcaccatc accatcgcgg cttcgggcca
     4021 ctgctgcagg atcagcgcgc ccgtcaccgc gatgctcatc agcgcattga tgttcaggtt
     4081 gccgttgcgg atcgcgagcc agcccttctt gtacgtcgtg aggccgcagg aggcgatcgc
     4141 gaggatcgcc agacccgccg ccagccagac cggggcgcca agccagctgg cagcctctga
     4201 ggccgccgcg gcgatacctg cgagcgccag cggccaccac ggcttgggcg gcgcgctggg
     4261 tgcgcgcgtg gacgcagcgc cgctgacggc gtccgccagc tcaggcgtga agtcgagcga
     4321 acggatcgcg gcgaggatcg agtcgagggc gtccggtgcg tgcacgacgg tcaacacgcg
     4381 ctgcatcagg ttgaagtcca tgctgcgcac gtagggcatg cggctgaatt ttttgcggat
     4441 cagcgcttct tcagtcgggc aatccatctg catgatgcgg atcgcggtgc gcacgtcgct
     4501 gccgacgact tcggattgcg ggagcttgat cggcctgggc atgacaggca tcgccgcgca
     4561 gcagtccgtg tcggcgtggc cgtggccgca gctgtcgccg cttgcgtgcg agtgcgcgtg
     4621 agccgattca ttggcgtgcg cctggtggcc atggttgcag cactcaccgt gctcatgagc
     4681 gtgtccgtgg tcccgttcct gagcatgcgc tgcatgggcg gggccgccgg atttgctcgg
     4741 gccgtggcac tgtgcgtcgt catccgcatg ctcgtgagta tgcccttcgt gcccgtgggc
     4801 acaggctttg gcctgttccg ggcggtccgc ttcggcgtgg ttcgcatgag gcatgggtgg
     4861 cttcctggtt cgatttgtgg tctagtaaac accttgaagc cactacaagg tcaaggccgc
     4921 aatccggagg gtccatgaaa atcggcgaac tggcgaaaat cgcccattgc acgaccgaaa
     4981 ccatccgttt ctacgaaaaa gagggcctgt tgcccgaagc ggagcgcacc gaggccaatt
     5041 accgcagcta tacggcgagg cacgtcgaac gcctgcgctt catccgcaat tgccgcgcgc
     5101 tggatatgac ccacgacgag atccgtgagt tgctgcgtct cacagacgca ccggcggacg
     5161 gctgcggcgg catcaatgcc ttgattgacg agcacattgc gcacgtcgac gcacgcattg
     5221 aagaattgaa gcaactgaaa gtgcaactga ccacgctgcg cgagcaatgc catggcgaac
     5281 aggccgttga agactgcggc atcgtccagg gccttaccga aatggacgtg agcgcaacgc
     5341 gcgcgcgaca tacgcatctg ggctgagtgc ttcgcccgta ctgacggcgg caatgacagc
     5401 agcaccgact gcggcaccgg ccgcagcaat gacggcggca ccggccgcac caccggccgc
     5461 ggcaatgaca gcagtcacgg ctgggccctt catccggcct gcctgataaa caatcgaatc
     5521 cagttggaga atcaacgtgt ttacctgtag aaaccagccc tgcggcgagc agtgggaaat
     5581 gtcggatgtc gtcatcaaga atgaagggca ggggcttctg ttccgttgcc cgatgtgcgg
     5641 tgcgcgcaat tacgtcgaac gtttcgacgg ggaagatggc tcggtgctgt acgagcagat
     5701 cgaaggccgt cccgccactg gccccatggc cgaatagtat ttagccttcc gcctcttcag
     5761 tcgatagcga gccccctcct atgaacagtc ccattcaagc cggcgcgccg tttagccaat
     5821 tacagctgcc gcccgccacg ctcgccaatc tgacgcaact cggctatgtc gagatgacgc
     5881 cgattcaggc cgcaagtctg ccgatcgcac tggccggcaa cgatctgatc gcccaggcga
     5941 aaaccggcag cggcaagacc gcggcgtttt cgctggcgct gctcaaccgc ctcgacgtgc
     6001 gcaacttcgc cgtgcaggcg atggtgctgt gcccgacgcg cgaactcgcc gatcaggtca
     6061 cgcaggaaat ccgccgtctg gcgcgcgccg aggaaaacat caaggtgttg acgctgtgcg
     6121 gcggcacgcc catgcgtccg caaaccgcca gcctcgaaca cggcgcgcac atcgtggtcg
     6181 gcacgccggg ccggatcatg gaccatctcg agcgcggcag cctgccgttg cagtcgctca
     6241 acacgctggt gctcgacgaa gctgaccgca tgctcgacat gggtttcttc gacgatatcg
     6301 ccaccgtcgt gaagcaatgt ccgaaagagc gccaaacgct gctgttctcc gcgacgtacc
     6361 cggaaggcat cgtcaagctg agccaacaat tcctgcgtaa tcccaaggaa gtgaagctcg
     6421 ccgagcggca cgacaacacc aagattcgtc agcgcttcta tgaagtgacc gaagaccagc
     6481 gcctgcatgc agtcggtctg ctgttgaacc actatcgtcc ggtgagcacg ctggcgttct
     6541 gcaataccaa acagcaatgc cgcgatctgc tcgacgtgct gcgcgcgcag ggttttcacg
     6601 cgctcgcgct gcacggcgaa ctcgatcaac gtgagcgcga tcaggtgctg attcagttcg
     6661 cgaaccgcag ttgctcggtg ctggtcgcta ccgacgtcgc ctcgcgcggt ctggacatcg
     6721 cggaactcga agcggtgatc aacgtcgacg tgacgccgga tccggaagtg cacgtgcacc
     6781 gtatcggccg cacggggcgc gccgatcagg aaggctgggc gctcagtctc gccagcatga
     6841 acgagatggg ccgcgtgggc agtctcgaac aggcgcagaa gcgtgaagtc gagtggcaca
     6901 agctggccga tctcaaaccg gcgagcaacg agcgcctgct gccgccaatg gaaacgttgc
     6961 agattctcgg cggccgcaag gaaaagatcc gcccgggcga cgtgctcggc gcgctgaccg
     7021 gcgaagcagg tttcgccggt tcgcagatcg gcaagatcaa cgtgaccgag atgtcgacct
     7081 atgtggccgt ggagcgcagc gtcgcgcgcg acgcactgcg caagctcagc gccggcaagg
     7141 tgaagggcaa gaaggtcaaa gtccgcatga tggacgacgc ttgatcgtca actagtttgc
     7201 ttttgacgtg ccgtttgctc gagaatgcgg ctgtgcgaga accatcgcat tggcgctgga
     7261 ttcgcggggt agacggcata cggttgacaa gaaaaaacgg ctgcgaccgc ctggtcgcgg
     7321 ccgagtggtc tgcatcatcg ctgcgcgcca atgcgccaat gcgccaatgc gccaatgcgc
     7381 caacgcgtca acgcaccaaa tcgcaccttc gcctcgccgc cgcgcatcac attacgtcat
     7441 acctcggctg tttcacgcgt tctccgcgtg gctgtatttc tcccgccaaa cccccgactc
     7501 cattgcctta gcctgcccca gcgcggcatt gcgcgagcca tgacgtaaac gcatgccgcg
     7561 catgacaaag tttcgtttgt gaccgttctt tggctgatgc ctaatcatcg aaccggatag
     7621 gaccgccgcg caaacggcgc tccgcgaatc cgctgccgga tggagaaagg gagaaagcct
     7681 tatgcagagc gccatgcaag cccgctcgaa actgcctgac gtaggcacga cgatttttac
     7741 cgtgatcggc caactggccg cgcaacacga tgcgctgaac ctgtcgcaag gcgcgccgaa
     7801 tttcgcgccg gacgcgaaac tgatcgacgg tgtcgcgcaa gccatgcgcg ccggtcataa
     7861 ccagtacgcg ccgatggccg gcatcgctac gctgcgcgaa gcgctcgccg acaaggtcga
     7921 gacgctatac ggcgtgcgct acgatccgtc gagcgaagtc accgtgatcg ccagcgccag
     7981 cgaaggcctg tattcgacca tcagcgccct ggtgcatccg ggcgatgaag tgatctattt
     8041 cgagccgtca ttcgatagct acggcccgat cgttcgcctg caaggcgcga cgccgatccc
     8101 gatcaaactg tcgttgagcg atttccgcgt gaactgggac gaggttgccg ccgcgatcac
     8161 gccgaagacg cgcatgatca ttatcaacac gccgcacaac cccaccgcga ccgtgttcag
     8221 cgaggccgac atcgcgcgtc tgaaggcagt gacgcgcaac accgacatcg tgattcttgc
     8281 cgacgaagtc tacgaacatg tggtgttcga cggcgccaaa catcagagca tggcgtgcga
     8341 cgccgaactc gcggagcgca gcgtgatcgt gtcgtcgttc ggcaagtcgt atcacgtgac
     8401 cggttggcgc gtcggctatt gcctcgcgcc cgccgcgctg atggacgaga ttcgcaaagt
     8461 ccatcagttc atggtgtttt ctgccgatac accgatgcag tacgcgttcg tcgatgcgtt
     8521 ggccaatcgc gagagctatc tcggcctatc cgcgttctat cagaagaagc gcgatctgct
     8581 cacgcatgca ctgcgcgatt cgcgcttcga gttgttgccg agcgaaggca gcttcttcat
     8641 gctcgcgcgt ttccggggct tttcagagga aggcgatagc gatttcgtgc tgcgcctgat
     8701 tcgcgatgca cgcgtcgcga cgatcccgct gtcggcgttt tataccgatg gcacggattc
     8761 cggcctgatc cgcctgagct tctcgaagga cgacgcgacc ttgatcgaag gcgcgcggcg
     8821 tttgtgcgaa atctgattgg ccggtttgtg cgaggttttc gtggcgagcg catgaagagc
     8881 tgacctgatc gcagaaggcc gcgatcagct tggcgcaagc gcgaaccggc agcatttttg
     8941 accgcagtat gatcatcgcg ccatggcgtc gttatgcatc agcgcgttgc atgccgcgtt
     9001 acaacaatat cgaatgaaaa gggacacgtt atgacattgc tcaagcctct cctggcgctg
     9061 gcctgcgcat tcacgtcgat tgccgcgtcg tcagccgcgt tcgccgccga tacgtcgacg
     9121 ctgcgtttcg gactcgaggc gcagtatccg ccgttcgaat cgaaagggcc gaacggtgaa
     9181 ctgcaaggtc tcgacatcga cgtcggcaat gccgtgtgcg tcgctgcgca catgacgtgc
     9241 aaatgggtcg aaacctcgtt cgacggtttg attcccgcgc tgcagggccg caagttcgac
     9301 gcgatcaatt cggcgatgaa tgccaccgaa cagcgccgtc gggcgatcga tttcacaacc
     9361 gtcgtgtatc gcgtgccgtc gcagttgatt gcgaagcgcg acagcggttt gctgccgacg
     9421 ccggcatcgt tgaagggcaa acgcgtcggt gtgttgcagg catcgattca ggaaaccttt
     9481 gcgaagacgc attgggaacc ggctggcgtc gccatcgtgc cgtatcagga ccagaaccag
     9541 gtttatgcgg acctcgtggc gggtcggctc gatgcgacgc tcgttctcgc gccggccggc
     9601 cagacgggtt ttctgtcgaa gcctagcggc gaaggctacg cgttcgtcgg tcaacctgtg
     9661 cgcgacgaca agattctcgg cagtggtatt gcgtatggga ttcggaaggg cgataccgcg
     9721 ctgcgtgatc agctgaacgc ggcgatcgcg aaggttcagg ctgatgggac ggtgaagacg
     9781 ctggccgcga agtatctcgg caatatcgac gtctcggcga agtaacttac tgaccgtgtg
     9841 tggcaggcat ggctcctgcc gcccgaaagt ggacgtccac gcttacgtac tgagcttcag
     9901 tgaagcgttg ccgcagcgcc gcagcacttc ttgaatttct tgccactgcc gcaagggcac
     9961 ggatcgttgc ggccgacttt gggttcggtg cgctggaccg tggcggctaa cgtgcgctcg
    10021 tagacggcct ggcggtgggg cagccagaaa cgatagatcg cggcgacgct ggcgggaatc
    10081 tgtttggtga gggcttcgtc cagttgcggc gtgccgatca attcttcgtc ttccagcgtg
    10141 agatctaggc cgcccatcag gcgaatcggc tccagccatt cccggccctg ctcgctatcg
    10201 aacagcgctt gccagtcctg ttcgacgagc aggaggcctt gcatgaagcc cattgcccac
    10261 gcttcgccgt cgatgtattc gctgccgctg tcgtcgatgc ggtggacacc aaacaccggc
    10321 tcgaacccat ccgggtcgtg ctcaacatcg tcgatgatgc cgttcatgta ccgcaagatt
    10381 agcccgagaa tgtgctgtgc ctgacccgct gattcgaacg caggcgtgtc atcctcgctg
    10441 gggccccaca cacccggcag ccagtaaccg ggcgatacgg tagttggccc gatggccatc
    10501 gcggtcagat aaccgtccag gcccatgatc gtgagggtct gttccgacgt caggtcggaa
    10561 atcagaaact catcgagttc gtcgaactct tcttcggaga gaggggaggc cagatcggcg
    10621 ggattgagtt tcgatttgga catgacgagg ctccggcgct gcaaatcgtg gatcccgata
    10681 gcttatcggg ggaatgcgga gtcggtctgc tcaaacggca ccggcgccac aaagcaaaaa
    10741 gcccagtcgc aaggaccggg ctttttgttg aatctttggt ggggcgtgag tgactcgaac
    10801 actcgaccta cggattaaga gtccgctgct ctaccaactg agctaacgcc cccaacagaa
    10861 gcgaaattat gccgaaactt tccgagattg ccaagcccct ttagcagatt tcctaaaaaa
    10921 atctgagagg cgcagtggcc cggcaccagc gctgcgagca gtcacgcgtc ggaagggcgc
    10981 acggttcgcc gtcccggtat tatgtcgcga caacttgccg cacgaagcct cgcgcggctt
    11041 tcgaccgggg accgccatgg atgacaaaga gattattgcg ttgctcgaca taata
//
LOCUS       DATASH010000925         1687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2135792,
            whole genome shotgun sequence.
ACCESSION   DATASH010000925 DATASH010000000
VERSION     DATASH010000925.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2135792"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1687)
                     /gene="dnaE"
                     /locus_tag="VHV99_24740"
     CDS             complement(<1..>1687)
                     /gene="dnaE"
                     /locus_tag="VHV99_24740"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007744169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX3638628.1"
                     /translation="CAVMAEARKPLLPICPKVRPGATEAETVQAMAEEGLRARLVAEA
                     LPEGEQEPYWQRLKFELGVIIQMGFPGYFLIVADFINWAKNNGVPVGPGRGSGAGSLV
                     AYALGVTDLDPLRYNLLFERFLNPERVSMPDFDIDFCQHGRDRVIQYVKEKYGADAVS
                     QIATFGTMAAKAAVRDIGRVLDLGYMFTDGIAKLIPFKPGKHVTIADAMKEEPLLQER
                     FDNEDEVHQLLELAQRVEGLTRNVGMHAGGVLIAPGKLTDFCPLYTQGDESGVVSQYD
                     KDDVEAVGLVKFDFLGLTTLTILDWAERYIRRLDPSKKDWSLAQVPLDDAASFSILKK
                     ANTVAVFQLESRGMQGMLKDAQPDRFEDIIALVALYRPGPMDLIQSFCARKHGREVVE
                     YPDPRVESVLKETYGIMVYQEQVMQMAQIIGGYSLGGADLLRRAMGKKKAEEMAEHRE
                     LFRQGAAKNGLTGDKADEIFDLMEKFAGYGFNKSHAAAYALLAYYTAWLKAHHPAEFM
                     AANMSLAMDDTDKVKILFEDCLTNKMAVLPPDVNLSAYRFEPVAEADGKRSKTI"
BASE COUNT          291 a          558 c          508 g          330 t
ORIGIN      
        1 ggattgtttt cgaccgcttg ccgtccgctt ccgctaccgg ctcgaaacga tatgcggaca
       61 gattgacgtc cggcggcagc acggccatct tgttcgtgag gcaatcttcg aacaggatct
      121 tgaccttgtc cgtgtcgtcc atggcgagcg acatattcgc cgccatgaat tcggccggat
      181 ggtgcgcctt cagccacgcg gtgtagtacg caagcagcgc atacgccgcc gcgtgcgact
      241 tgttgaagcc gtagcccgcg aacttctcca tcaagtcgaa gatttcgtcc gctttgtcgc
      301 cggtcagccc gttcttcgcg gcgccctggc ggaacagctc gcggtgctcg gccatttcct
      361 cggccttctt cttacccatc gcgcggcgca gcaagtcggc accgccgagc gaataaccgc
      421 cgatgatctg cgccatctgc atcacctgct cctggtagac catgatgccg taggtctctt
      481 tcagaacaga ttcgacgcgc ggatccggat actccaccac ttcgcggccg tgcttacgtg
      541 cacagaagct ctggatcagg tccattgggc ccggacggta caacgcgacc agcgcgatga
      601 tgtcctcgaa gcggtcaggc tgcgcgtctt tcagcatgcc ttgcatgccg cggctttcca
      661 gctggaacac ggcgaccgtg tttgctttct tcaggatcga gaacgacgcc gcgtcgtcga
      721 gcggcacctg cgcgagcgac cagtctttct tcgacggatc gagacggcgg atatagcgct
      781 cggcccaatc cagaatcgtc agcgtggtga ggcccaaaaa gtcgaacttc acgaggccga
      841 cggcttcgac gtcgtccttg tcgtactggc tcacgacgcc gctttcgtcg ccctgcgtat
      901 agagcggaca gaaatcggtc agtttgccgg gcgcgatcag cacgccgccg gcgtgcatac
      961 cgacgttacg cgtgaggcct tccacgcgct gcgcgagttc gagcaactgg tgcacctcgt
     1021 cttcgttgtc gaagcgctcc tgcaacagcg gctcttcctt catcgcgtcg gcgatggtga
     1081 cgtgtttgcc cggcttgaac ggaatcagct tggcgatgcc gtccgtgaac atgtagccga
     1141 gatcgagcac gcggccgatg tcgcgcaccg ccgctttcgc cgccatcgtg ccgaaggtgg
     1201 cgatctgcga cacggcgtcc gcgccgtact tctccttgac gtactgaatc acacggtcgc
     1261 ggccgtgctg gcagaagtcg atgtcgaagt cgggcatcga cacccgctcc ggattcagga
     1321 aacgttcgaa cagcaggttg tagcgcagcg ggtcgagatc ggtcacgcca agcgcgtagg
     1381 cgaccagcga acccgcgccc gagccccggc ccggtccgac cggcacgccg ttgttcttcg
     1441 cccagttgat gaagtccgcc acgatcagga agtagcccgg aaagcccatc tggatgatga
     1501 cgccgagctc gaatttcaac cgctgccaat acggctcctg ctcaccctcg ggaagggcct
     1561 ccgcgaccag ccgcgcgcgc aggccttcct cggccatggc ctgcaccgtc tccgcctcgg
     1621 tggcgccggg gcggaccttc gggcagatag gcagcagcgg cttgcgcgcc tcggccatga
     1681 ctgcgca
//
LOCUS       DATASH010000926         3431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_7081134,
            whole genome shotgun sequence.
ACCESSION   DATASH010000926 DATASH010000000
VERSION     DATASH010000926.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7081134"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /gene="secD"
                     /locus_tag="VHV99_24745"
     CDS             <1..1035
                     /gene="secD"
                     /locus_tag="VHV99_24745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013338068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEX3638629.1"
                     /translation="RIIDASAGFDEHQRPSVNIRLDSAGGRAVRSVSRDNIGKQMAMV
                     LFEKGKGEVLTVATIQSELGDRFQITGQASPQGAADLALLLRAGSLAAPMDIIEERTI
                     GPSLGADNIRKGFHSVVWGFVAIAVFMIAYYMLFGVISMIGLSVNLLLLIAVLSMLQA
                     TLTLPGIAAIALALGMAIDANVLINERVREELRHGAPPQLAIQNGYAHAWATILDSNV
                     TTLIAGLALLAFGSGPVRGFAMVHCIGILTSMFSAVFFSRGIVNLWYGGKKKLKSLAI
                     GQVWRPQPEGIDGAAAYLGNEDAATDTARAVAAASAKPKAKAAAQARAKPSVRRRDAS
                     NTPQKPGSSR"
     gene            1054..2004
                     /gene="secF"
                     /locus_tag="VHV99_24750"
     CDS             1054..2004
                     /gene="secF"
                     /locus_tag="VHV99_24750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011852175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEX3638630.1"
                     /translation="MEFFRFRKDIPFMQRALIFNAISLLTFLAAVFFLVHRGLHLSVE
                     FTGGTVIEVQYPGAAPLEPVRGTLNKLGYPDAQVQNFGTSRDVLIRLPLKQGYTSAQQ
                     SDQVMGALKGQDAQVQLQRVEFVGPQVGKELATDGLLALACVVVGIVIYLSFRFEWKY
                     AVAGVIANLHDVVIILGFFAFFQWEFSLSVLAAVLAVLGYSVNESVVIFDRIRETFRR
                     ERKMTVVEVINHAITSTMSRTIITHGCTQMMVLSMFLFGGPTLHYFALALTVGILFGI
                     YSSVFVAAALAMWFGVKREDLIKDKKERADPDDPNAGAQV"
     gene            complement(2096..3430)
                     /locus_tag="VHV99_24755"
     CDS             complement(2096..3430)
                     /locus_tag="VHV99_24755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006760287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3638631.1"
                     /translation="MMMCGTLIIAVLPTYQSIGLAAPVILVLARLMQGFSAGGEFGSA
                     TAFLAEHVPGRRGFFAVIIAKLFFPAGNDTVSLLLTLGTFGVSFFMRPLGAIVIGAYA
                     DRAGRKAALTLSILLMMCGTLIIAVLPTYQSIGLAAPVILVLARLMQGFSAGGEFGSA
                     TAFLAEHVPGRRGFFASWQVASQGLTTLLAAGFGVLLTGKLSPEQMGSWGWRVPFFFG
                     LLIGPVAWYIRTKLDETPEFLAAETTTTPLRDTFTSQKLRLVIAIGVVVLGTVSTYLV
                     LFMPTYGVKQLGLAPSVAFAAIALTGLIQMVFSPLIGHLSDRHGRTTIMLISALLLLV
                     LIYPAFVYLVAHPTFGTLIALQIVFGFLMTGYFAALPGLLSEMFPVQTRTTGMSLAYN
                     IAVTIFGGFGPFIIAWLISFTGSKAAPSYYMIFAAAISLVALMAARRKLGFR"
BASE COUNT          662 a         1105 c         1069 g          595 t
ORIGIN      
        1 cgcatcatcg acgcgtcggc aggcttcgac gaacatcagc gtccgtcggt caacatccgt
       61 ctggattcgg ccggtggccg cgcggtgcgc agcgtttcgc gcgacaacat cggcaagcag
      121 atggccatgg tgctgttcga gaagggcaag ggcgaagtgc tgacggtggc gaccatccag
      181 tcggaactgg gtgaccgctt ccagatcacc ggccaggcct cgccgcaagg cgccgccgac
      241 ctcgcgctgc tgctgcgcgc cggttcgctg gctgcgccga tggacatcat cgaagaacgc
      301 acgatcggcc cgagcctcgg cgcggacaac atcaggaagg gcttccactc ggtggtgtgg
      361 ggcttcgtgg cgatcgccgt cttcatgatc gcgtactaca tgctgttcgg cgtgatctcg
      421 atgatcggcc tgtcggtcaa cctgttgctg ctgatcgccg tgctgtccat gttgcaagcc
      481 acgctcaccc tgccgggtat cgcggctatc gcgctcgcgc tcggtatggc aatcgacgcg
      541 aacgtgctga tcaacgaacg ggtgcgtgaa gaactgcgcc acggcgcgcc gccgcaactg
      601 gcgatccaga acggctacgc gcatgcatgg gcgacgattc tcgactcgaa cgtgaccacg
      661 ctgatcgccg gtctcgccct gctcgccttc ggctccggcc cggtgcgcgg cttcgcgatg
      721 gtccactgta tcggcatcct gacgtcgatg ttctcggcgg tgttcttctc gcgcggcatc
      781 gtcaatctct ggtacggcgg caagaagaag ctcaagtcgc tggccatcgg tcaggtgtgg
      841 cgtccgcagc ccgaaggtat cgacggcgct gccgcctacc tcggcaacga agatgctgcg
      901 accgacacag cacgagccgt cgccgcggca agcgccaagc ccaaggccaa ggctgcggcc
      961 caggcgcgcg ccaagccgag cgtgcgccgc cgcgacgcgt cgaatacgcc gcagaaaccg
     1021 ggttcatccc gctgaacccc ggagaacaag accatggaat ttttccgttt tcgcaaagac
     1081 attccgttca tgcagcgtgc gttgatcttc aacgcaatct cgctcctgac gttccttgcc
     1141 gctgtgttct tcctggtaca tcgcgggctg catctgtcgg tggaattcac cggcggtacg
     1201 gtcatcgagg tgcagtatcc tggcgccgcg ccgctcgaac cggtgcgcgg cacgctcaac
     1261 aagctcggct atcctgacgc gcaagtgcag aacttcggca cctcgcgcga cgtgctgatc
     1321 cgtctgccgc tcaagcaggg ttacacctcg gcgcaacaga gcgatcaggt gatgggcgcg
     1381 ctgaagggtc aggacgcgca ggtgcagttg cagcgtgtcg agttcgtcgg cccgcaggtt
     1441 ggcaaggaac tcgctaccga cggcctgctc gcgctggcct gcgtggtggt cggcatcgtg
     1501 atctacctgt cgttccgctt cgaatggaag tacgcggtgg ccggcgtgat cgcgaacttg
     1561 cacgacgtgg tcatcattct cggcttcttc gcgttctttc agtgggagtt ctcactgtcg
     1621 gtgctggccg cggtgcttgc ggtgctcggc tactcggtga acgaatcggt cgttatcttc
     1681 gaccggattc gcgaaacttt ccgccgcgaa cgcaagatga ccgtggtcga ggtgatcaac
     1741 cacgccatta ccagcacaat gtcgcgcacg atcatcaccc acggctgtac gcagatgatg
     1801 gtgctgtcga tgttcctgtt cggcggccct acgctgcact acttcgccct ggcgctgacg
     1861 gtgggtattc tgttcggtat ctactcgtcg gtgttcgtcg ccgcggcgct cgccatgtgg
     1921 ttcggcgtga agcgcgaaga cctgatcaag gacaagaagg aacgcgccga tccggacgat
     1981 ccaaacgcag gcgcgcaagt ctgatcctgc cgtacgcaga catttgactc gagtgcaata
     2041 aagaaaggcc ggttctctcg aaccggcctt ttacgtttac cgccgttact gggctttatc
     2101 tgaacccaag cttacggcgc gccgccatca gcgccaccag actgatcgcc gcggcaaaaa
     2161 tcatgtagta gctcggcgcg gccttcgaac ccgtgaagct gatcagccac gcgatgatga
     2221 acggcccgaa gccgccgaag atcgtcacgg caatgttata cgcgagcgac atgccggtcg
     2281 tccgtgtctg caccgggaac atctccgaga gcaaacccgg cagcgccgcg aagtaacctg
     2341 tcatcagaaa gccgaacacg atctgcagcg cgatcagcgt gccgaaggtc ggatgcgcga
     2401 ccagatacac gaaggccggg tagatcagca cgagaagcag cagcgccgag atcagcatga
     2461 tcgtggtgcg cccgtggcgg tccgacagat ggccgatcag cggcgagaac accatctgga
     2521 tcagaccggt cagcgcaatc gccgcgaacg cgaccgacgg cgccagcccc aactgcttca
     2581 cgccgtacgt cggcatgaac agcaccagat aggtcgacac ggtaccgagc accaccacgc
     2641 cgatcgcaat cacgaggcgc agcttctggc tggtgaaggt atcgcgcagc ggcgtcgtgg
     2701 tggtttccgc cgcgagaaac tcgggtgttt cgtcgagctt ggtgcggatg taccacgcaa
     2761 ccggtccaat cagcaggccg aagaagaacg gcacgcgcca gccccacgag cccatctgct
     2821 ccggcgagag cttgccggtc agcagcacgc caaagccagc tgcgagcagc gtggtgagcc
     2881 cctggctcgc gacctgccag ctcgcgaaga aacctcgccg ccccggcacg tgctccgcca
     2941 gaaaagccgt ggcactgccg aactcgccgc ccgctgaaaa gccttgcatc aggcgcgcca
     3001 gcacgaggat cacgggtgcg gcgaggccga tgctctgata cgtcggcagc accgcgatga
     3061 tcagtgtgcc gcacatcatc agcaggatcg acaacgtgag cgccgccttg cgtccggcgc
     3121 ggtcggcgta agcgccgatc acgatcgcgc cgagcggccg catgaaaaac gacacgccga
     3181 aggtgccgag tgtcagcagc aacgagacgg tgtcattacc cgccgggaaa aacagcttcg
     3241 cgatgatgac cgcgaaaaaa ccacgccgcc ccggcacgtg ctccgcgaga aaagctgtgg
     3301 cactgccgaa ctcgccgccc gctgaaaagc cctgcatcag acgcgccagc acgaggatca
     3361 cgggcgcggc gaggccgatg ctctgatacg tcggcagcac cgcgatgatc agtgtgccgc
     3421 acatcatcag c
//
LOCUS       DATASH010000927         6547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4904735,
            whole genome shotgun sequence.
ACCESSION   DATASH010000927 DATASH010000000
VERSION     DATASH010000927.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6547
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4904735"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VHV99_24760"
     CDS             <1..538
                     /locus_tag="VHV99_24760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018425038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEX3638632.1"
                     /translation="AGAKRAIGERAAEFVPDGASLIIDSGSTTQAVARALLDRHRLSV
                     YTNDWRIALLLGRRNDNRVTLLGGELSDIEDATFGLDTVHQLTQYHADFAFVGAGGIS
                     PDGYLTDYSRMAAEVRSRMIAAADMVVVVADHSKFGRVTPVRINGFESARYLVTELAP
                     EKTLGKAITAHGPEIMIA"
     gene            complement(575..1684)
                     /locus_tag="VHV99_24765"
     CDS             complement(575..1684)
                     /locus_tag="VHV99_24765"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015603459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HEX3638633.1"
                     /translation="MLGNDPILLTPGPLSTSPQTREAMLRDWGSWDAAFNRMTHDVCA
                     ELVKIVHGENDYVCVPLQGSGTFAVEAALGTLVPRQGCVLVPNNGAYCARLVRILQRM
                     GIAYVELALREDEPVSAAAMEDAFNRNSRITHVAQVHLETSAGLLNPLDDIAAVCQRH
                     GKSLIVDAMSSFGALPIDLRRGGIDALISASGKCLEGVPGMGFVIVRRSVLGECEGRS
                     PSLALDLHDQYVYMEKTTQWRFTPPTHVVAALRRALDQFIAQGGQAARGARYRQNCAA
                     LVDGMKALGFEPFLKPEVQASVIVTFHAPRDPAWNFADFYAAVREAGYVLYPGKLTEM
                     ETFRVGCIGAIDENELRNAVAAIGRTVEKLGIRVR"
     gene            2052..3143
                     /gene="phnS"
                     /locus_tag="VHV99_24770"
     CDS             2052..3143
                     /gene="phnS"
                     /locus_tag="VHV99_24770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_function: GO:0033226 - 2-aminoethylphosphonate binding
                     [Evidence IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX3638634.1"
                     /translation="MTKTNSLHATAGLARKLLPALVAAAAFGGMAMQAHAADAVVLYT
                     ADGLENLYKDVLPAFEKKEGVKVNIVTAGSGEVVNRATIEKDQPKADVLVTLPPFIQQ
                     ADQSGLLQAYQSANYKNVPAIAKASNGTWATFVNNYFSFAINPDVVKTQPKTFADLLH
                     PDYSGKIAYSNPATAGDGMAVIILTSALMGEDKAFDYLKKLEQSAKFHTKGTGYLDVL
                     LSRNEIAFANGDLQMDLDDAANGGLSLKPIFLAASAGGKPTTFQLPYAIALIKNGPNQ
                     AEGKKLIDYLMSTDVQAKVPDIFGIPARTDVPLTGKNGEAVKQAIAGVTLIPVDWNQV
                     MAKKADWTTRWKNEVIGSSGKQLEVVKPK"
     gene            3233..4333
                     /gene="phnT"
                     /locus_tag="VHV99_24775"
     CDS             3233..4333
                     /gene="phnT"
                     /locus_tag="VHV99_24775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate ABC transport system
                     ATP-binding subunit PhnT"
                     /protein_id="HEX3638635.1"
                     /translation="MNTASLANPGALGTSPDALDAARSNTPGGVQIDHLTVRFGTRTV
                     LDDLSLTIQRGELLTVLGRSGCGKTTLLRFIAGFIEADGLAGTLTVAGHDLTHVPPHK
                     RNLGLLFQSYALFPHLSVFDNVAFGLRARRTPSKDVTRRVADALKLVQLGDAGHHMPA
                     QLSGGMQQRVALARALVIEPDVLLLDEPLSALDANLRASVRSELKALHERLPNLTIVC
                     VTHDQDDALVLSDRTLLMREGRIAQLGTPQQLYDTPNDGFVARYLGAANLLPPQVAFG
                     MGDVRYNERERLACLRPEALRIVPLGEGQLHGAITSVEWYGAVLSVSVALDAMPNEPV
                     LVTMQRGHGMMPEKGARVSLRYEADDVVLINP"
     gene            4311..5258
                     /locus_tag="VHV99_24780"
     CDS             4311..5258
                     /locus_tag="VHV99_24780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018435709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate ABC transporter permease
                     subunit"
                     /protein_id="HEX3638636.1"
                     /translation="MSSLSTPDTPDSMSAAAAADARRTASAASHTAAVKRRERAAQWH
                     LLFLAVVVLGPLVIYPLVRLVLLSLTGPHGLSLQAYSAFFGNPETRGVVGTTLWILLA
                     SAGLASLLGVALASMLFFKPFPGARMVTRFLELFVAFPSFLVAFTLIFLYGSQGSVSI
                     GLQRLFHLEAPPLDFLFGVGGVILAEVVFYAPFVVRPTLASFATLDMRLIEAARSLGA
                     SGWMVAFKVILPLAWPGIAAGTILCFLLTLNEFGILLVLGSAHLITLPVAIYSSATVD
                     LDLPTAAAGAVVMLIMSLSLYALYRQVNRRKVKGAGHGR"
     gene            5248..6111
                     /gene="phnV"
                     /locus_tag="VHV99_24785"
     CDS             5248..6111
                     /gene="phnV"
                     /locus_tag="VHV99_24785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate ABC transport system,
                     membrane component PhnV"
                     /protein_id="HEX3638637.1"
                     /translation="MAVELDPTVLPVMHKKARPVRRSLPVRVLGGLFLGFAALLCFWL
                     FVLPVVVVALSSVAGHWSGTILPDSLSMRWFERLGSSDFDALTTSLEIGMGVAVLGTI
                     LGLWLALALEGRDRRGLGAFVDTIAMMPNGVPSVVLGLAVLIAYHKRPFDLSNSAAIV
                     VLVQLALVLPFCYRCAAAALRPELTVLREAAASLGAPPSMVLRRVVLPQLVPAIRASL
                     ALGFALSLGELGATLTVYPPGFATVPIVVVGQVERGYYLPASALSLILLMVSLAALLL
                     IAARVPRRRVD"
     gene            6186..>6547
                     /locus_tag="VHV99_24790"
     CDS             6186..>6547
                     /locus_tag="VHV99_24790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007587365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX3638638.1"
                     /translation="MTEYMLTASVDPIEVVRRHSLTTLVRDEIERHIVEWALEPCDKL
                     NEADWAARLQVSRGPVREAFRALEQAGLVRTEKNRGVFVRTVSLTEADEIYAVRAVLE
                     EAACRMLAARIDARELAA"
BASE COUNT         1106 a         2058 c         2109 g         1274 t
ORIGIN      
        1 cgccggtgcg aagcgggcga tcggcgagcg cgcggccgag ttcgtgccgg acggcgcttc
       61 gctgatcatc gattcgggta gcaccactca ggcggtggcg cgggcattgc tcgaccggca
      121 tcgcctgtcg gtctatacga acgactggcg tatcgcgctg ctgctcggcc gccgcaacga
      181 taaccgcgtg acgctgctcg gcggcgaact gtcggatatc gaagatgcca ccttcggcct
      241 cgacacggtc catcagctga cgcaatacca cgcggacttc gcctttgtcg gcgcgggcgg
      301 catttcgccg gacggctacc tgaccgacta cagccgcatg gccgccgaag tgcgcagccg
      361 tatgatcgcc gcggccgaca tggtggtggt cgtcgccgat cactcgaagt ttgggcgcgt
      421 gacgccggtg cgcatcaacg gcttcgagtc ggcgcgttat ctggtcaccg aactggcgcc
      481 ggagaagacg ctcggtaagg cgatcacggc gcatgggccg gaaatcatga ttgcctgaag
      541 cgcaaggcgg gcccacccgc catcgcgccg cgcattacct cacacggata cccaacttct
      601 cgaccgtacg gccgatcgcc gccaccgcgt tgcgtagctc gttctcatcg atcgcaccga
      661 tgcagccgac gcggaacgtt tccatttcag tgagcttgcc cggatagagc acatagcccg
      721 cctcgcgcac cgcggcataa aagtccgcaa aattccacgc agggtcgcgc ggcgcgtgga
      781 acgtgacgat caccgacgcc tgcacttcag gcttcagaaa cggctcgaag ccgagcgctt
      841 tcatcccgtc caccaacgcc gcgcagttct gcctgtagcg tgcgccgcgc gccgcttgtc
      901 cgccttgcgc gatgaactgg tcgagtgccc gacgcaacgc ggcgaccacg tgagtcggcg
      961 gcgtgaagcg ccactgcgtc gtcttctcca tataaacgta ctgatcgtgc agatccagcg
     1021 cgagcgatgg cgaacgtcct tcgcattctc ccagcacact gcgtcgcaca atcacgaagc
     1081 ccattcccgg cacgccttcc aggcatttgc cgctcgccga aatcagcgcg tcgatgccac
     1141 cgcgccgcaa gtcgatcggc aacgcgccga acgaactcat cgcgtcgacg atcaggctct
     1201 tgccgtgccg ctgacatacc gcggcgatgt cgtcgagcgg attgagcagg ccagcgctgg
     1261 tttccagatg cacctgcgcg acgtgggtga tgcgcgaatt gcgattgaag gcgtcttcca
     1321 tggcggcggc gctgaccggt tcgtcctcgc gcagggccag ttcgacataa gcgatcccca
     1381 ttcgctgcaa tatcctgacc agacgggcac aatacgcgcc gttgttcgga accagcacgc
     1441 agccttgacg cggcaccagc gtgccgagcg ccgcttcgac cgcgaacgtg ccgctgcctt
     1501 gaagcggcac gcatacatag tcattttcgc catgtacgat cttcaccagc tcggcgcata
     1561 cgtcatgagt catgcggttg aacgcggcgt cccacgaacc ccagtcgcgc aacatcgcct
     1621 cgcgggtttg cggcgaagtg gagagggggc ccggcgtcaa caggattggg tcgtttccaa
     1681 ggatcacaca gcctccgtca tcagattgaa tgtccgtcgc ccacgtgggg tgacgaaaag
     1741 attgatttgg atattattgg cgaattgtgt cattttttgg aagccgacag aatagtcatg
     1801 gcgttgtcat ggaaacctgt aatccttgcc gtgcccgatc aggcacggca ggcagcgcga
     1861 gtgtcgtaga tgggctgtag tcaggctgga aaagaagcgg caaagttgtc ggcaatctgc
     1921 aatccatacg aaaactgcac gacaactggc tgcgggcaac acgcaagcgg cacggcgcta
     1981 ccgccgttca atcacaacag gtatcgggaa aatcccgacc gtctggtttt ttgcctttcg
     2041 gagagagcga catgacaaag acgaattccc ttcacgccac agctggcctc gcacgcaaac
     2101 tgttgccggc attggtcgcc gccgcagcgt tcggtggcat ggcgatgcaa gcgcatgcgg
     2161 ctgacgcagt ggtgctgtac accgccgacg gcctcgaaaa cctctacaag gatgtgctgc
     2221 cggccttcga aaagaaggaa ggcgtgaagg tcaacatcgt cacggcaggc agcggcgaag
     2281 tggtgaaccg cgccaccatc gaaaaggatc agccgaaggc cgacgtgctc gtcacgctgc
     2341 cgccgttcat ccagcaagcc gaccagagcg gcctgttgca ggcttatcag agcgccaact
     2401 acaagaacgt gccggccatc gccaaggcgt cgaacggtac atgggcgacg ttcgtgaaca
     2461 actacttctc gttcgcgatc aacccggacg tcgtcaagac ccagccgaag accttcgccg
     2521 acctgctgca cccggactac agcggcaaga tcgcttactc gaacccggcc acggctggcg
     2581 acggcatggc cgtgatcatc ctgacctcgg cgctgatggg cgaagacaag gcgttcgatt
     2641 acctgaagaa gctcgagcag agcgccaagt tccacaccaa gggcaccggc tacctcgacg
     2701 tgctgctctc gcgtaacgaa atcgccttcg ccaacggcga cttgcagatg gatctggacg
     2761 atgcggccaa cggcggtctg tcgctcaagc cgatcttcct cgcagccagc gccggcggta
     2821 agccgaccac tttccagctg ccgtatgcga tcgccctgat caagaacggc ccgaatcagg
     2881 cagaaggcaa gaagctgatc gactacctga tgtcgactga cgtgcaagcc aaggtgccgg
     2941 acatcttcgg tatcccggcc cgtaccgacg tgccgctcac cggcaagaac ggcgaagcag
     3001 tcaagcaggc catcgccggc gtgacgctga ttccggtcga ctggaaccag gtgatggcga
     3061 agaaggctga ctggaccact cgctggaaga atgaagtgat cggttcgtcg ggcaagcaac
     3121 tcgaagtcgt caagccgaag taagccggac cattcgtcat agagtcaggc ggcgcttccc
     3181 gagttcgtgc gaggcgccgc gtcagtatta atccagttgg aggatgaacc cggtgaatac
     3241 ggccagcctt gctaaccccg gcgcgctcgg cacctcgccg gacgcgctcg acgccgcgcg
     3301 ttcgaacacg ccgggcggcg tgcagatcga tcatctgacc gtgcgtttcg gaacgcgcac
     3361 cgtgctcgac gatctgtcgc tgaccattca gcgcggcgaa ttgctcaccg tgctcggccg
     3421 cagcgggtgc ggcaagacca cgctgttgcg cttcatcgcc gggttcattg aagcggacgg
     3481 actggccggc accttgacgg ttgccggcca cgacctgacg catgtgccgc cgcacaagcg
     3541 caatctcggc ttgctgtttc agagctatgc gctgttcccg catctgtcgg tgttcgataa
     3601 cgtggctttc gggttgcgcg cccgccgcac gccgtcgaag gatgtcacgc ggcgtgtcgc
     3661 cgatgccttg aagctcgtgc aactgggcga cgccgggcat cacatgcccg cgcaattgtc
     3721 gggcggcatg cagcagcgcg tggccttggc gcgcgcgctt gtgatcgagc ccgacgtgct
     3781 gttgctcgac gaaccgcttt ccgcactcga cgccaatctg cgcgcctcgg tgcgttccga
     3841 actgaaggcg ctgcatgagc gcctgccgaa tctgacgatc gtctgcgtga cgcacgatca
     3901 ggacgacgcg ctggtactct ccgaccgtac gctgctgatg cgcgaaggcc gcatcgcgca
     3961 actcggcacg ccgcagcagt tgtacgacac gccgaacgac ggctttgtcg cgcgttatct
     4021 gggcgcggcc aacctgctgc cgccgcaggt cgcattcggc atgggcgacg tgcgctacaa
     4081 cgaacgtgag cggttggcct gtctgcgtcc ggaagcgctg cggatcgtgc cgctcggcga
     4141 agggcagttg catggcgcga ttacgtcggt cgaatggtac ggcgcggtgc tgtcggtgtc
     4201 ggtcgctctg gacgcgatgc ctaacgagcc cgtgctggtc accatgcagc gtggccacgg
     4261 catgatgccg gagaagggcg cgcgtgtttc actgcgttat gaggctgacg atgtcgtcct
     4321 tatcaacccc tgatacgcct gattcgatga gcgcggcggc cgccgccgat gctcgccgca
     4381 ctgccagtgc ggcctcgcac acggcagcgg tgaagcggcg cgaacgcgcg gcgcaatggc
     4441 acctgctgtt cctggcggtg gtcgtgctcg gaccgctggt tatttatccg ctggtgcgtc
     4501 tggtgctgct gagtctcacc ggtccgcacg gtttgagcct gcaagcctac tccgcgtttt
     4561 tcggcaatcc ggaaacgcgc ggcgtggtcg gcacgacgct gtggattctc cttgccagtg
     4621 cggggctcgc gtccttgctc ggcgtcgcgt tggcgtcgat gctgtttttc aagccgtttc
     4681 ccggcgcgcg gatggtcacg cgtttcctcg agctgttcgt cgcgttcccg tcgtttctgg
     4741 tcgccttcac gctgattttt ctgtacggct cgcaaggttc ggtcagcatc ggcttgcagc
     4801 ggctgttcca tctggaggcg ccgccgctcg attttctgtt cggcgtgggt ggcgtgattc
     4861 tcgccgaagt ggtgttctac gcgccgttcg tagtgcgtcc gacgctggca tcgtttgcca
     4921 cgctcgacat gcgtctgatc gaggctgccc gcagtctcgg cgcaagcggc tggatggtcg
     4981 cgttcaaggt gatcctgccg ctcgcgtggc cgggcatcgc tgcgggcacg atcctgtgtt
     5041 tcctgctgac gttgaacgag ttcggcattc tgctcgtgct cggcagcgcg catctcatca
     5101 cgctgccggt cgcgatctac agttccgcga cggtcgacct cgatctgccg actgccgccg
     5161 ccggcgcggt cgtcatgctg atcatgtcgt tgtcgctgta cgcgttgtac cgtcaggtca
     5221 accgccgcaa ggtgaaagga gcgggccatg gccgttgaac tcgacccgac cgtcttgccg
     5281 gtcatgcaca agaaggcgcg gccggtgcgc cgcagtctgc cggtgcgcgt gctgggcggc
     5341 ctgtttctcg gctttgccgc gttgctgtgc ttctggctat tcgtcctgcc ggtggtggtg
     5401 gtcgcgctat cgagcgtggc cgggcactgg tcgggcacga tcctgccgga cagtctcagc
     5461 atgcgctggt tcgaacggct gggttccagc gacttcgacg cgctgaccac gagcctggaa
     5521 atcggcatgg gcgtcgccgt gctcggcacg attctcgggc tatggctggc gctggcgctc
     5581 gaaggacgcg accggcgcgg tctcggcgcg ttcgtcgaca ccatcgcgat gatgcccaac
     5641 ggcgtgccga gcgtggtgct tgggctggct gtgttgatcg cgtaccacaa gcgtccgttc
     5701 gatctgtcga actcggcggc gatcgtcgtg ctcgtgcagc tcgcgctggt gctgccgttc
     5761 tgctaccggt gcgcggccgc cgcattgcgc cctgaattga ccgttctgcg cgaagcggca
     5821 gcgagtctgg gtgcgccgcc gtcgatggtg ctgcgtcgcg tcgtgctgcc gcagttggtg
     5881 ccggccattc gcgcgagcct cgctttgggc ttcgcgctgt cgctcggcga acttggcgcc
     5941 acgctcaccg tgtatccgcc cggtttcgcg acggtgccga tcgtcgtggt cggtcaggtg
     6001 gagcgcggtt attacctgcc ggcctccgcg ttgtcgctga ttctcctgat ggtctcgctt
     6061 gccgcgctgc tgctgatcgc ggcgcgtgtt ccgcgtcgtc gggtggactg acgcgcatct
     6121 tctgagggtt gctttggcgt gccggcggcg cgtcgacaca tatcgatgtg gcaaaatttg
     6181 tcaaaatgac cgaatatatg ctaactgctt ctgtcgatcc gattgaggtt gtgcgcaggc
     6241 attcgctgac gacgctggtc cgcgacgaaa tcgaacggca catcgtcgaa tgggcgctcg
     6301 agccgtgcga caaactcaac gaagccgatt gggccgcgcg tttgcaggta tcgcgcgggc
     6361 cggtgcgcga ggcgttccgc gcgttggagc aggccggcct ggtgcgcacc gagaaaaacc
     6421 gcggtgtgtt cgtgcggacg gtgtcgttga cggaagcgga tgagatttac gcggtgcgtg
     6481 cggtgctcga agaggcggcg tgccggatgc tggctgcgcg tatcgatgca cgcgaactgg
     6541 ccgcgtt
//
LOCUS       DATASH010000928        12992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6685595,
            whole genome shotgun sequence.
ACCESSION   DATASH010000928 DATASH010000000
VERSION     DATASH010000928.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 12992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12992
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6685595"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1918)
                     /locus_tag="VHV99_24795"
     CDS             complement(<1..1918)
                     /locus_tag="VHV99_24795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HEX3638639.1"
                     /translation="MAAALVLVACATVSQAEGSTASNSSTTSASGTSSTSGTTSPSSP
                     SSPSSTSSPSSTSSPPIDDLSPSTMERDVHLFVIQKDGSVEEHDDTVLRANTTSGVDD
                     IAQRYVWFNKDIEQVQLLGAETIDPNGVAHPVGPEAIRDVQEPRSAGAPSFEDGVLRT
                     VIFPGVEPGSRVHLAFRKTRTKPLQAGTFAYLVEPTRDPVEMQRLIFDLPADVPLYAD
                     ARGYVALPPVTANGRTRYEFDYRHGPYAPLEAGAVGYANWGDRLMVSTVPDFASFAAR
                     YRGPASDATASDPAIVRLAQSLTAEASDPRAKAQILYDWMRLNIRYVALFLGETAAIP
                     HKAIDILHNRYGDCKDHVALYGALLAALGIRSEAVLLNLGPYYSLPDVPGYGASAINH
                     AIVWIPELSLFADTTAGGVGFGYLPPSVMDRPVLLVDAGVLSRTPATQMRERTARLQI
                     DIDESGAANYAYRVEDDGYTAELERNTFRRATRQRTQQVAANRLLLTGLHGTAQLRVG
                     DVGATGGPFSTSMQGRVEHFVWTDGTTAVPALTSLSGGMAGQVQAWLAEPARTQPWAC
                     IGGEFDETLEMALPGFARVTDVPPDATVKDRFFEFSSRYVFDPTARVLQVRRHLRATF
                     GHQMCSADEFAEVKD"
     gene            complement(1942..2841)
                     /locus_tag="VHV99_24800"
     CDS             complement(1942..2841)
                     /locus_tag="VHV99_24800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018439061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX3638640.1"
                     /translation="MGKIAVTLQQALAQRAREGMRGSTSARALAQGEGWEVADVLCTF
                     GPRDQPFEERHAGVCIAMVVAGSFGYRAGTGRELLTPGALLLGNPGDCFECGHRHAAG
                     DRCIAFRYAPAYFERLAADAGIASGNLNFRLARIPSLAALSPLTARACAGALEAGETV
                     WGELAAELAVATLKAAGGLTHAEPPASATAWSRVAQTVRLIDDTPGATHTLTSLAAAA
                     GLSEFYFLRTFQRVSGVTPHQYVLRARLRAAALRLHDDNAKVVDIALDCGFNDLSNFN
                     HAFRAEFASSPRAWRARGRARRA"
     gene            2950..3513
                     /locus_tag="VHV99_24805"
     CDS             2950..3513
                     /locus_tag="VHV99_24805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018439062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638641.1"
                     /translation="MMNMLDDTLDREFHAALSAPARAADIAAADDVYGWLIGSWDMDV
                     LHYRVDLGSARRRGEIHFGWVLEGRAVQDVWIMPPQSERHNGLEPGGTMYGTTLRVWD
                     PALRAWRVTYLNPLTGQRDELTGRRVGNDLVQIGTHADGTPIRWNFTDITSDSFRWTG
                     VALAQDGVTWTLEAEFHARRRRQVARH"
     gene            complement(3574..4239)
                     /locus_tag="VHV99_24810"
     CDS             complement(3574..4239)
                     /locus_tag="VHV99_24810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638642.1"
                     /translation="MEDSDELLLPVWRANLVLLTREVGAATRLARMMTFSASYLKLIL
                     SGQREFSEEFVRGIEAVTGLPNGWMNASHTQEDIPAKAREAIDNEQPLARFRGTAHPV
                     RKKTVLRPPEPIFGQTGPAKRVEEETLDAETHRRQAHFRKVRDVAMQDVRRFERHLSH
                     APVDLASMRSKVEEVIAAADLDDPIQADLAGRLEQIEKHRHLLLRHVERLQALLGQIG
                     EGD"
     gene            4556..4693
                     /locus_tag="VHV99_24815"
     CDS             4556..4693
                     /locus_tag="VHV99_24815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638643.1"
                     /translation="MKKIILALAAAVAALSAIAPAQAYEHHHKECHKVRVHHHWENRC
                     H"
     gene            complement(4980..5801)
                     /gene="hutG"
                     /locus_tag="VHV99_24820"
     CDS             complement(4980..5801)
                     /gene="hutG"
                     /locus_tag="VHV99_24820"
                     /EC_number="3.5.1.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050129 - N-formylglutamate deformylase
                     activity [Evidence IEA];
                     GO_process: GO:0019557 - histidine catabolic process to
                     glutamate and formate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate deformylase"
                     /protein_id="HEX3638644.1"
                     /translation="MTASSIATTPPVLSLHQGSLPLLISIPHLGTQIPADIAATMTPV
                     AQRTDDCDWHLDRLYAFARRMGASILAPTYARYVIDLNRPPDGANLYPGQDTTGLLPV
                     DTFDKEPLYREGHLPTDAELARRRDAYWKPYHDALVSELAALKAKHGKVLLWEAHSIR
                     SHVPRFFEGRLPDFNFGTSNGASAVTGVGEELAAVVERHGGYSAVANGRFKGGYITRQ
                     YGQPSQGVHAVQLELSQITYMEEQMPYAYDETLAMKVQPLLEELVATALERVKAA"
     gene            complement(5892..7289)
                     /locus_tag="VHV99_24825"
     CDS             complement(5892..7289)
                     /locus_tag="VHV99_24825"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HEX3638645.1"
                     /translation="MMQANQSLFAEHAYLPDGWRRNVLLEWDAHGTLTSVTPDTARVP
                     DGVEKAAGPVLPGMPNLHSHAFQRAMAGLTEYRAAAGTAATDNFWSWRDLMYRFAARI
                     TPEGLAAVAQWLYIEMLKAGYTSVCEFHYVHHTPDGSRYPNQAELAQRVVDAASTSGI
                     GMTMLPVLYQYSGFGSRAPREDQRRFINTPESLLELLGTVRAARPESAALRYGVAPHS
                     LRAVSADSLHELLSGIDRSAPVHIHIAEQTAEVDDCLETEGARPVQWLLDRFNVDSRW
                     CLVHATHVDANETLALAKSGAVAGLCLTTEANLGDGIFPAHEYLDAQGRIGVGSDSHI
                     GVDWRAELRLLEYGQRLTRRQRNVLASARATHVADRLFDAALDGGAHATGRAVGALQA
                     GKRADWLVLDPAHSSIAEHAPNAWLSGVVFCEHGETPIRDVYTGGDKVVDNRRHRDEE
                     GAYARYRGALADLLR"
     gene            complement(7300..8523)
                     /gene="hutI"
                     /locus_tag="VHV99_24830"
     CDS             complement(7300..8523)
                     /gene="hutI"
                     /locus_tag="VHV99_24830"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEX3638646.1"
                     /translation="MKQTVWHHLKLCPQGDPQHTLPDAAIAVENGKIAWLGAASELPA
                     EYATWPREDLHGAWVTPGLVDCHTHLVYGGQRADEFAQRLAGVSYEEIARQGGGIVST
                     VRATRAADEDSLFRQSAARLEPLLAEGVTVVEIKSGYGLDLASERKMLRVARRLGERY
                     PVTVYTTFLGAHALPPEFAGRADAYIDEVCNTMLPALADEGLVDAVDVFCERIGFSLE
                     QSERVFKAAERHKLPVKMHAEQLSNGGGTALAARHHALSADHLEFLDEAGVAAMKASG
                     TVAVLLPGAYYFIRETQLPPLDLLRRYQVPIAISTDSNPGTSPTMSLLLMMNMATTLF
                     RMTVPEVLQGVTSHAARALGKADTHGSLEAGRAADFAVWSVATLAELAYWIGRPLCAR
                     VVRAGETVYARRELS"
     gene            complement(8636..9238)
                     /locus_tag="VHV99_24835"
     CDS             complement(8636..9238)
                     /locus_tag="VHV99_24835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HEX3638647.1"
                     /translation="MATTTLIRGADLVAAPWKNGGGVTREVAAFPLHAGLDIFTWRVS
                     IADVAQAGPFSRFAGIDRTLVLLSGAGMLLDETGDTDVHAVKTHALTQPLDIAQFAGE
                     ARIDARLVDGATRDFNLMVRRGAAVGETEVWRGATQRTLSADVVLLFCASGSLQVALR
                     GDAQPRALEADDTLRIDTPNALPCVVEGAGALLAISIRYM"
     gene            complement(9271..10956)
                     /gene="hutU"
                     /locus_tag="VHV99_24840"
     CDS             complement(9271..10956)
                     /gene="hutU"
                     /locus_tag="VHV99_24840"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015600548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEX3638648.1"
                     /translation="MNNPKHIDPRLDPTRTIRAPRGEKTCKTWIAEAAYRMIQNNLDP
                     EVAEHPHALVVYGGIGRAARNWDCFDQILASLKDLNEDETLLIQSGKPVGVFRTHADA
                     PRVLLANSNLVPHWATWEHFHELDRKGLMMYGQMTAGSWIYIGSQGIVQGTYETFFSV
                     ANQHFNGDPSGRWILTGGLGGMGGAQPLAATMAGFSMIAVECDETRIDFRLKTRYVDK
                     KAQTLDEALAMLEEAKKAGKPVSIGLLGNAADVFAECVARDITPDCVTDQTSAHDPIH
                     GYLPQGWNVEDWRERMKTVPDSIVLPAKQSMAKQVQAMLTLQERGAATLDYGNNIRQM
                     ALEMGVENAFDFPGFVPAYIRPLFCEGKGPFRWVALSGDPEDIYKTDAKVKELIPDDP
                     HLHNWLDMARERIAFQGLPARICWVGVKDRYRLGQAFNEMVRNGELKAPIVIGRDHLD
                     TGSVASPNRETESMKDGSDAVSDWPLLNALLNTAGGASWVSLHHGGGVGMGFSQHAGV
                     VIVADGTDAAKERLGRVLFNDPATGVMRHADAGYELAQETAREAGLNLPMLGR"
     gene            complement(11049..11744)
                     /gene="hutC"
                     /locus_tag="VHV99_24845"
     CDS             complement(11049..11744)
                     /gene="hutC"
                     /locus_tag="VHV99_24845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine utilization repressor"
                     /protein_id="HEX3638649.1"
                     /translation="MNAPAYQGIKDFILGRIHAGEWAEGDQVPSENELAREFNVARMT
                     VNRALRELTSEQVLTRVQGSGTFVARPKYESTLVAIRSISDEIVARGHRYQAKVLHIG
                     ASIADEALAEEMRVSAGSPVFHSRVLHFENDEPVQLEERWVNPAVAPEYALQDFTNTT
                     PNQYLVRVAPLQRVEYRIEALAADADTRELLTMDELEPCLVLHRRTWSQSQVASIANL
                     WHPGSRYRFTGHF"
     gene            complement(11782..>12992)
                     /gene="hutH"
                     /locus_tag="VHV99_24850"
     CDS             complement(11782..>12992)
                     /gene="hutH"
                     /locus_tag="VHV99_24850"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEX3638650.1"
                     /translation="LGRGHSGIRREVMEALITLYNADVLPVIPVKGSVGASGDLAPLA
                     HMSATLLGVGEVFARGERMPATEGLALVGLKPLTLQAKEGLALLNGTQASTALALYNM
                     FAIEDLYRTALVAGALSVDAAAGSVKPFDARIHELRGHQGQIDAAAAYRSLLHGSGIN
                     VSHADCDKVQDPYSLRCQPQVMGACLDQMRHAAEVLLLEANAVSDNPLIFPDTGEVLS
                     GGNFHAEPVAFAADNLALAVAEIGALAERRIALLIDATLSGLPPFLVRDGGVNSGFMI
                     AHVTAAALASENKTLAHPASVDSLPTSANQEDHVSMATFAARKLGDIAENVANILSIE
                     LLAAAQGVDLRAPHKTSPSLQKVMDAVRKDVAHYELDHYFAPDIEAVTHLVQDGAIAK
                     LSPFSFASEQ"
BASE COUNT         2333 a         4282 c         4070 g         2307 t
ORIGIN      
        1 cgtccttcac ttcggcgaat tcgtcagcgg aacacatctg atgcccaaac gtggcgcgaa
       61 ggtgtcgtct gacctgcagg acccgcgcgg tgggatcgaa cacgtagcga gaagaaaact
      121 cgaagaaccg atccttcacc gtggcatccg gcggaacatc cgtcacgcgc gcgaagcccg
      181 gcagcgccat ctccagcgtc tcgtcaaact ccccgccgat gcacgcccat ggctgcgtcc
      241 gggcgggctc cgcaagccat gcctgcacct gccccgccat gccgccggac aagctggtca
      301 gcgcgggcac ggccgtcgtg ccgtcggtcc agacaaagtg ttcgacgcgt ccttgcatcg
      361 acgtggaaaa cgggccgccg gttgcgccca catcgcccac gcgcaactgc gcggtgccat
      421 gcaagccggt cagcaataga cgattcgccg cgacctgttg cgtacgctgg cgcgtcgccc
      481 gtcgaaacgt attgcgttcg agctccgcgg tatagccatc gtcctccacg cggtaagcat
      541 agttcgccgc cccgctctca tcgatatcga tctgcagacg cgcagtgcgc tcgcgcattt
      601 gcgtggccgg cgtgcgcgac aacaccccgg cgtcgaccag caggaccggc cggtccatga
      661 cgctcggcgg caaatagcca aagccgacgc cgcccgccgt agtgtcggcg aacaacgaca
      721 actcaggaat ccagacgatt gcatgattga tcgcgcttgc accgtatccg ggcacgtccg
      781 gcaaactgta gtaaggcccg agattgagca gcacggcctc gctgcggatg ccgagcgcgg
      841 cgaggagcgc gccgtacaac gcgacgtggt ccttgcagtc gccatagcgg ttgtgcagga
      901 tgtcgatcgc cttgtgcggg atcgccgccg tttcgccgag aaacagcgcg acgtagcgga
      961 tattcaaacg catccagtcg tacaggatct gcgccttcgc gcgcgggtcg ctggcctcgg
     1021 cggtcaggct ctgtgcgagt cgaacgatcg ccgggtcgct cgccgtcgcg tcggaggcag
     1081 gaccgcgata gcgcgcggca aaactcgcga agtccggcac cgtcgacacc atcaaccggt
     1141 cgccccagtt cgcatagccg accgcgcccg cttcgagcgg cgcatagggg ccgtgccggt
     1201 agtcgaactc gtagcgggtg cggccgtttg ccgtcaccgg tggcagcgcg acgtagccac
     1261 gcgcatcggc ataaagcggc acgtcggcgg gcaggtcgaa gatcagacgc tgcatctcga
     1321 ccgggtcgcg cgtcggttcg accaggtagg cgaaggtgcc ggcctgcagg ggcttcgtgc
     1381 gggtcttgcg aaacgccaga tgcacgcgcg agccaggctc gacgcccgga aagatcaccg
     1441 tgcgcagcac cccgtcttcg aaactcggcg cgcccgccga gcggggttcc tgcacgtcgc
     1501 ggatcgcctc ggggccgacc ggatgcgcga cgccgttcgg gtcgatcgtt tcggcgccca
     1561 gcaattgcac ctgctcgata tccttgttga accacacgta gcgttgcgca atatcgtcga
     1621 cgccgctcgt cgtgttggcc cgcaggaccg tgtcgtcgtg ttcttcgacg gagccgtctt
     1681 tctggatcac gaagagatgg acgtcgcgct ccatcgtcga gggggagagg tcgtcgattg
     1741 gggggcttga ggtgcttgag gggcttgagg tgcttgaggg gcttgagggg cttgaggggc
     1801 tggtggtgcc ggacgtactg gacgtacctg aagcgctggt ggtgcttgag ttgctggcgg
     1861 tactcccctc agcctgcgag accgttgcgc acgcaaccag caccaacgcg gcggccagcc
     1921 tgatgaccag agcgcaccgc gtcatgcgcg acgcgccctg ccccgcgcgc gccatgcccg
     1981 cggactcgac gcaaactccg cgcgaaacgc atgattgaaa ttcgacagat cgttgaagcc
     2041 gcaatcgagc gcgatatcga cgaccttcgc gttgtcgtcg tgaaggcgca gcgccgccgc
     2101 tcgcaaacgt gcccgcagca catattgatg cggcgtcacg ccgctcacgc gctggaacgt
     2161 acgcaggaaa tagaactcgc tcaggcccgc cgcggcagcg aggctcgtga gcgtatgcgt
     2221 tgcgccaggg gtgtcgtcga tcaggcgcac cgtctgcgcg acgcgcgacc aggccgtggc
     2281 gcttgcaggt ggttccgcat gcgtcaaccc gcccgccgcc ttgagcgtcg cgacggccag
     2341 ttcggccgcg agttcgcccc acaccgtctc gcctgcttcc aacgcgccgg cacaagcccg
     2401 cgcagtcagc ggcgacaacg cggctagcga tggaatgcgc gcgagcctga agttgagatt
     2461 gccgctcgcg atgcccgcat cggcggcgag ccgctcgaag tacgcgggtg cgtagcggaa
     2521 ggcgatgcag cgatcgccgg ccgcatgccg atggccacat tcgaaacagt cacccggatt
     2581 gccgagcagc agcgcgcccg gcgttagcaa ctcacgcccc gtgcccgccc ggtagccgaa
     2641 cgaaccggcg accaccatcg cgatgcatac accggcgtga cgttcttcga acggctggtc
     2701 gcgcgggccg aaggtgcaaa ggacgtccgc gacctcccag ccctcgccct gggccagcgc
     2761 acgcgcgctc gtgctgccgc gcatgccttc ccgtgcgcgc tgcgcgagtg cctgctgcaa
     2821 agtgacggca attttcccca agatgcacct cggctaagac cgtatcgtgg ttttagcaca
     2881 tcggcggccg gcatggtttt gcccattccg gcgagagcgc tttccactgg aacacccaaa
     2941 ggagcacaca tgatgaacat gctggacgac accctcgacc gcgagtttca cgcggcattg
     3001 agcgccccgg cgcgcgccgc cgacatcgcc gcggcggacg atgtttacgg ctggctgatc
     3061 ggcagttggg acatggacgt gctgcattac cgcgtcgatc tgggcagcgc gcgccgccgc
     3121 ggcgagatcc atttcggctg ggtactggag ggccgcgccg tgcaggacgt ctggatcatg
     3181 ccgccgcaga gcgagcgtca caacgggctc gaacccggcg gcacgatgta cggcaccaca
     3241 ctgcgagtgt gggacccagc actgcgggcg tggcgtgtga cctacctcaa tccgctgact
     3301 ggccagcgcg atgaactgac cggccggcgc gtcggcaatg atctcgtgca gatcggcacg
     3361 cacgccgacg gcacgccgat tcgatggaat ttcaccgata tcacgagcga ttcgttccgc
     3421 tggaccggcg tcgcgcttgc gcaggatggc gtgacgtgga cgctcgaggc ggaatttcac
     3481 gcgcggcgca ggcgtcaggt ggctcgacat taggctgcgg cgtggcggcg agcggcttca
     3541 ggcccacatg aaacagaaac cctgctgcgg gcatcaatcc ccctccccga tctgcccaag
     3601 caaagcctgc aagcgctcca catgcctgag cagtaaatgc cgatgctttt cgatctgctc
     3661 cagccggccg gccagatcag cctgaatcgg atcgtcgaga tcggcggcgg cgatcacctc
     3721 ttccactttc gagcgcatcg acgcaaggtc caccggcgca tgactcaaat gccgctcgaa
     3781 acgccgcacg tcctgcatcg ccacatcgcg gaccttgcga aaatgcgcct gacgccgatg
     3841 cgtttcggcg tcgagcgtct cttcttccac gcgtttggcc gggcccgtct gcccgaaaat
     3901 cggctcgggc ggccgcaaca cggtcttctt gcgcaccgga tgcgcggtgc cgcgaaaccg
     3961 cgcaagcggc tgttcattgt cgattgcttc gcgcgccttg gccggtatgt cctcttgcgt
     4021 gtgtgacgcg ttcatccagc cgtttggcaa gcccgtgacc gcctcgatac cgcgcacgaa
     4081 ctcctcgctg aattcgcgct ggccggacaa gatcagtttc agatagctcg cggaaaaggt
     4141 catcatgcgc gcgagacgcg tcgccgcgcc cacctcgcgg gtcagcagca ccaggttcgc
     4201 tcgccagacc gggagcagta attcgtcgga atcttccatc gctgggtctt ccatcttttg
     4261 cccgatgtac ggacgacgcg atacacacgc gtcatcgaat tgaaagggta gccgtttcac
     4321 ggcgtggcgc gcaatcccgg agcgaacata tggcggctcc agatgtgttg aaattacgcg
     4381 ctacgctgcg ccgccgctca gggataaaac gccacgaagc tcgtcgtttt gagttgaccc
     4441 atgcattcgg ccagcgttcc tgttttgagc gcgcgatctg tggcatcctt gcgcgtcgtt
     4501 gcggcacgct tcgtgctgca gcgttagcac catcacgtcg attaaggatt ttgtaatgaa
     4561 gaaaatcatt cttgccctcg cagcagccgt agctgccctc agcgcgatcg ctcccgccca
     4621 ggcctacgag catcatcaca aggaatgcca caaagtgcgc gtccaccatc attgggaaaa
     4681 ccgctgccac taatggccgg gtctcgcctg cgagggtgaa ccgagggtga ataaaaagcc
     4741 ccgctttacg cggggctttt tttacgcccg ggcgcgtgtg ccggcatgtt ctgcaacgct
     4801 ctataagttc tgcgcgaaac cgccagcatg caatcacgga cacatgctgt tacacctttg
     4861 cagtgtaggg atgcctcaag acgacctcgc gcgacgcgaa gcgccatgaa cgccgtaggg
     4921 cgatcagatg aaaaacgacg acagcgccgc cgagcggctc gacgcgcgcg agtggcgggt
     4981 caagctgcct tgacgcgctc caaagccgtc gccaccagtt cttcaagcag aggctggacc
     5041 ttcatggcca gcgtttcgtc atacgcatac ggcatctgct cctccatata cgtaatctgc
     5101 gacaactcga gctgtaccgc gtgcacaccc tgcgagggct gcccatactg ccgcgtgata
     5161 tagccgccct tgaagcgccc attggcgaca gcggaatacc cgccatgccg ctcgaccacc
     5221 gcggccagtt cctcgcccac tcccgtcacc gcgctggcgc cgttcgacgt gccgaaattg
     5281 aaatccggca gacgtccctc gaagaaccgc ggcacatgcg agcggatcga atgcgcctcc
     5341 cacagcagca ccttgccgtg cttcgccttc agcgcggcaa gctcgctcac cagcgcatcg
     5401 tgatagggct tccagtaagc atcgcgccgg cgtgcgagtt cggcgtcggt cggcagatgg
     5461 ccttcgcggt acaagggttc cttgtcgaag gtatcgaccg gcagcaagcc ggtggtgtcc
     5521 tgccccggat agagattcgc gccatcgggc ggacggttca gatcgatcac ataacgcgcg
     5581 tacgtcggcg ccaggatcga tgcacccatg cgccttgcaa acgcgtagag acggtcgagg
     5641 tgccagtcgc agtcgtcggt gcgctgcgcg accggcgtca tcgtcgcggc gatatcggcg
     5701 ggaatttgcg tgcccagatg cgggatcgaa atcagcaacg gcaggctgcc ttgatgcagc
     5761 gacaaaaccg gcggagtagt tgcaatagac gaagcagtca tgtcagtccg gaatcgatgg
     5821 gatcgaagga aatctcagtt tattcggcaa ctcacgcgcg catttcgctt gggcatgtgg
     5881 cattcagccg ctcacctgag caaatcggcg agcgccccac gatagcgcgc gtaagcaccc
     5941 tcttcatcgc gatgccgacg gttgtcgacg accttgtcgc cgcccgtgta gacgtcgcga
     6001 atcggcgtct cgccatgctc gcagaataca acgcccgaga gccacgcgtt cggcgcatgc
     6061 tcggcgatgc tcgaatgagc cggatcgagt accagccagt cggcacgctt gccagcctgc
     6121 agcgcgccga cggcacggcc ggttgcgtgc gcgccgccgt cgagcgcggc gtcgaacaaa
     6181 cgatcggcca catgcgtggc ccgcgccgat gccagcacgt tgcgctgacg ccgcgtaaga
     6241 cgctggccgt attcgagcaa acgcaattcc gcccgccagt cgacgccgat atggctatcc
     6301 gaaccgacgc cgatgcgccc ttgcgcgtcg aggtactcgt gcgccggaaa aatgccgtcg
     6361 ccgagattgg cttcggtggt caaacacaaa ccggcgaccg cgccgctttt cgcgagcgca
     6421 agcgtttcgt tcgcatcgac gtgcgtggca tgcacgaggc accagcggct atcgacattg
     6481 aagcgatcga gcagccattg caccggccgc gccccttcgg tttcgaggca gtcgtcgact
     6541 tcggcggttt gctcggcgat atggatgtga accggcgcgc ttctatcgat accgctcagc
     6601 aattcatgca acgagtcggc cgataccgcg cgcaacgaat gcggcgccac gccataacgc
     6661 aacgccgcgc tctcaggccg cgccgcgcgc accgtgccga gcagttcgag cagactctcc
     6721 ggcgtattga taaagcgccg ctgatcttcg cgcggtgcac gcgaaccgaa accgctgtac
     6781 tgatacagca cgggcagcat cgtcatgccg atgccgctcg tcgaagccgc gtccaccaca
     6841 cgctgcgcca gctccgcctg gttcggatag cggctgccat ccggcgtgtg atgcacgtag
     6901 tggaattcgc agaccgacgt atagcccgct ttcaacatct cgatgtacaa ccactgcgcg
     6961 acggcagcga gtccttccgg cgtgatccgc gcggcaaacc gatacatcag atcgcgccag
     7021 ctccagaagt tatcggtggc cgctgtgccc gctgcggcgc gatattcggt caggccggcc
     7081 atcgcgcgtt gaaacgcatg cgagtgaaga ttcggcatgc cgggcaatac gggaccagcg
     7141 gctttttcaa cgccgtcggg aacccgggcc gtatcgggcg taaccgacgt cagcgtgcca
     7201 tgggcgtccc attccagcag tacgttgcgg cgccagccat cgggcagata ggcgtgctcg
     7261 gcgaatagcg attggtttgc ttgcatcatc tctcagaccc tagctcaatt cacgccgtgc
     7321 ataaacggtc tcgcccgcgc gcaccactcg cgcgcacaac ggacgcccaa tccagtaagc
     7381 cagctcggcc agcgttgcca ccgaccacac cgcgaaatcc gccgcgcgcc ctgcttccag
     7441 cgaaccgtgc gtatctgcct tgccgagcgc acgcgccgca tgcgaggtca cgccttgcag
     7501 cacttcgggc acggtcatgc ggaacagcgt ggtcgccatg ttcatcataa gcagcagcga
     7561 catggtgggc gatgtgcccg gattgctgtc ggtggaaatc gcgatcggca cctgatagcg
     7621 ccgcagcaag tccaaaggcg gcagttgcgt ctcgcggatg aagtagtacg cgccgggcag
     7681 cagtacggcg acggtgccgg atgctttcat cgcggcgaca cccgcttcgt cgaggaattc
     7741 gaggtgatcc gcggacagcg catgatggcg cgcggccaat gccgtgccgc cgccgttcga
     7801 caactgctcg gcgtgcattt tcaccggcaa cttatggcgt tcggcggctt tgaacacgcg
     7861 ctcgctttgt tccagcgaaa aaccgatacg ctcgcaaaac acgtcgaccg cgtcgaccag
     7921 gccttcgtcg gcgagcgcgg gcagcatcgt gttgcagact tcgtcgatat aggcgtcggc
     7981 ccttcctgcg aattcgggcg gcaatgcatg cgcaccgaga aacgtcgtat agaccgtcac
     8041 cgggtagcgc tcgcccaacc ggcgcgcaac gcgcagcatc ttgcgttcgc tggcgagatc
     8101 gaggccgtag ccggatttga tttcgaccac cgtcacgcct tcggcgagca gcggttcgag
     8161 gcgcgcggcc gattggcgga acagcgagtc ttcgtcggcg gcacgcgtgg cgcgcaccgt
     8221 cgagacaata ccgccgccct gccgcgcgat ttcctcatag ctgacccccg cgaggcgctg
     8281 cgcgaattca tcggcgcgct gcccgccgta caccagatgc gtatggcaat cgacgagacc
     8341 cggcgtcacc cacgcaccgt gcagatcttc gcgcggccat gtggcgtatt cggcgggtaa
     8401 ttcggaggcc gcaccgagcc atgcgatctt gccgttttca acggcaatcg cggcatcggg
     8461 cagcgtgtgt tgaggatcgc cctgggggca cagtttcagg tgatgccaga cggtttgctt
     8521 catcgcgtgc tctttgcagc tcgttgcgtg tctgtggtgg ttcgcggcgc gcttcgcctg
     8581 gcttgcttcg ccaggcctta ggcgcgtgcg cgaatcgtcg tcatcgttac ggtcatcaca
     8641 tatagcggat gctgatcgcg agcaacgcgc ccgcgccctc gaccacgcat ggcagcgcgt
     8701 tcggtgtgtc gatgcgcagc gtgtcgtcag cttcgagcgc gcgcggttga gcgtcgccac
     8761 ggagcgcgac ctgaagcgaa ccgcttgcgc agaacaacag caccacgtcg gcggacaacg
     8821 tacgctgcgt agcaccgcgc cacacttctg tctcgcccac cgccgcgcca cggcggacca
     8881 tcaggttgaa gtcgcgcgtg gcgccgtcga cgaggcgtgc atcgatccgc gcttcgcccg
     8941 caaactgcgc gatatcgagc ggctgagtca atgcatgggt cttcacggcg tgcacgtcag
     9001 tgtcgcctgt ttcgtcgagc aacatgcccg cgccggacaa caacaccaac gtgcggtcga
     9061 tacccgcaaa acgcgagaac ggaccggcct gcgccacgtc ggcgatgctc acgcgccacg
     9121 tgaagatgtc gagccccgcg tgtagcggaa acgccgccac ctcacgcgtg acgccgccgc
     9181 cgtttttcca cggcgccgcc acgaggtcgg cgccgcgaat cagcgtcgtg gttgccatgt
     9241 tatcgacaag cgggctgtga agcgccacga tcaacgaccc agcatcggca gattcaaacc
     9301 ggcctcgcgc gccgtttcct gggcgagttc atagcccgca tccgcgtgac gcatcacacc
     9361 ggtcgccggg tcgttgaaca gcacgcggcc gaggcgttct ttggctgcat ccgtgccgtc
     9421 ggcgacgatc acgacgcccg cgtgctggct gaagcccatg ccgaccccgc cgccatgatg
     9481 cagcgagacc cacgacgcgc cgcctgcggt gttcagcagt gcgttgagca agggccagtc
     9541 gctgaccgcg tccgagccgt ccttcatcga ttcggtctcg cgattcgggc tggccaccga
     9601 accggtatcg aggtgatcgc ggccgatcac gatcggcgcc ttcagctcgc cgttcctgac
     9661 catttcgttg aaggcctggc ccaaacgata acggtctttc acgccgaccc agcagatccg
     9721 cgccggcagc ccctggaacg cgatgcgttc gcgcgccatg tcgagccagt tgtgcagatg
     9781 cggatcgtcc gggatcagtt ccttgacctt cgcatcggtc ttgtagatgt cttccggatc
     9841 gcccgacagc gcgacccacc ggaacgggcc cttgccttcg cagaacaacg gacggatata
     9901 cgccggcacg aagcccggga aatcgaacgc gttttccacg cccatttcca atgccatctg
     9961 acggatgttg ttgccgtagt cgagcgtggc cgcgccgcgt tcctgcagcg tcagcatcgc
    10021 ctgaacctgc ttggccatcg actgcttggc gggcagcacg atgctgtccg gcacggtctt
    10081 catacgctcg cgccagtctt cgacattcca gccttgcggc aggtaaccgt gaatcggatc
    10141 gtgcgcgctg gtttgatcgg tcacacaatc cggcgtgatg tcacgcgcca cgcattcagc
    10201 gaacacatcc gctgcgttgc cgagcaggcc gatcgacacc ggcttgcccg ctttcttcgc
    10261 ctcttcgagc atggcgagcg cttcgtcgag cgtctgcgct tttttgtcga cgtagcgcgt
    10321 cttcagacgg aagtcgatac gcgtttcatc gcattccacc gcgatcatcg agaagccggc
    10381 catggtcgct gccagcggtt gcgcgccgcc catgccgccg agaccgcccg tcagaatcca
    10441 gcggcccgac ggatcgccgt tgaaatgctg attcgccacc gagaagaacg tttcgtaggt
    10501 gccctgcacg atgccctggc tgccgatgta gatccagctg cccgccgtca tctgcccgta
    10561 catcatcaag cccttgcgat cgagttcgtg gaagtgttcc cacgtcgccc aatgcggcac
    10621 cagattcgaa ttcgccagca agacgcgcgg cgcgtccgca tgcgtgcgaa acacaccgac
    10681 cggcttgccc gattgaatca gcagcgtctc gtcttcgttc aggtccttca gcgacgcgag
    10741 gatctgatcg aagcaatccc agttacgcgc ggcacggccg atgccgccgt acacgaccag
    10801 cgcatgcgga tgctcggcga cttccgggtc cagattgttc tggatcatcc ggtacgcggc
    10861 ttccgcgatc caggtcttgc aggttttttc gccgcgcggt gcgcggatcg tgcgggtcgg
    10921 gtccagacgc gggtcgatgt gtttcgggtt gttcatggtg gccctcggaa tgcgtgaaaa
    10981 tgttcgggag aagatcggaa aataagccgg gacaacgagt ctgaaaaaga gtctgaaaag
    11041 cggcgaaatc agaaatgtcc ggtgaagcga tagcggctgc ccggatgcca gagattggcg
    11101 atcgaagcga cctggctttg cgaccacgtg cgccgatgca gcacgagaca cggttcgagt
    11161 tcatccatgg tcagcaactc gcgcgtgtcc gcgtccgccg ccaatgcttc gatgcggtac
    11221 tcgacccgct gcaacggcgc cacgcgcacc agatactgat tcggcgtggt gttcgtgaag
    11281 tcctgcaagg catactcggg cgcgaccgcc gggttgaccc agcgttcttc gagctgcacc
    11341 ggttcgtcgt tttcgaaatg cagcacgcgt gaatgaaaca ccggactgcc cgcgctcacc
    11401 cgcatttctt cggcgagcgc ttcgtcggcg atgctcgcgc cgatgtgcag caccttcgcc
    11461 tgataacgat gaccgcgcgc gacgatctcg tcggagatgc tgcggatcgc caccagcgtc
    11521 gattcgtact tgggccgggc cacgaacgtg cccgagccct gcacacgcgt gagcacctgc
    11581 tccgaagtca actcgcgcaa cgcgcggttg accgtcatgc gcgcgacgtt gaattcgcgc
    11641 gcgagctcgt tttcggaggg cacctggtcg ccttcagccc attcgcccgc atggatgcgg
    11701 ccgaggatga agtccttgat gccctggtag gccggtgcgt tcattggctt gatgtgcgtg
    11761 cttgaaacgt gttgtgcgag cttactgttc ggacgcgaac gagaacgggc tcagcttcgc
    11821 gatcgcgccg tcttgcacga gatgcgtcac cgcctcgata tccggtgcga agtagtgatc
    11881 gagctcgtaa tgcgcgacat ccttgcgcac cgcgtccatc actttctgca agctcgggct
    11941 ggtcttgtgc ggcgcgcgca gatcgacgcc ttgcgccgcg gcgagcaact cgatcgacag
    12001 aatgttcgcc acgttttcag cgatgtcgcc gagcttgcgc gcggcgaacg tcgccatcga
    12061 aacgtgatct tcctggttcg cggaggtggg cagcgaatcg accgaagccg gatgcgcgag
    12121 cgtcttgttc tcggaggcga gcgcggcagc cgtcacgtga gcgatcatga agccggagtt
    12181 cacgccgccg tcgcgcacga ggaacggcgg caggcccgac agcgtcgcgt cgatcagcag
    12241 cgcgatgcgg cgctcggcca atgcgccgat ttcagcgaca gccagcgcga ggttgtcggc
    12301 agcgaaggca accggctcag cgtggaagtt gccgcccgac agcacttcgc ctgtgtccgg
    12361 gaaaatcagc ggattgtcgg acacggcgtt ggcttccagc agcagcacct cggccgcatg
    12421 gcgcatctga tccagacacg cgcccatcac ctgcggctgg cagcgcaggc tgtacgggtc
    12481 ctgcaccttg tcgcaatcgg cgtgcgaaac attgatgccc gagccgtgca acagcgaacg
    12541 gtacgccgcg gccgcgtcga tctgaccttg atggccgcgc agttcgtgaa tgcgcgcgtc
    12601 gaacggcttg accgaacccg cggctgcgtc caccgacaac gcgcccgcca ccagggcggt
    12661 gcggtacagg tcttcgattg cgaacatgtt gtagagcgcg agcgccgtcg aggcttgcgt
    12721 gccgttcagc agcgccagac cttccttcgc ctgcaacgtc agcggtttca ggccgaccag
    12781 cgcgaggcct tcggtggccg gcatgcgctc gcccctggcg aacacttcgc cgacgccgag
    12841 caaggtggcc gacatatgcg cgagcggcgc gaggtcgccc gatgcgccga ccgagccctt
    12901 gaccggaatc accggcagca cgtcggcgtt gtacagcgtg atcagcgctt ccatcacttc
    12961 gcggcggatg cccgagtggc cgcggccgag gc
//
LOCUS       DATASH010000929        11974 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5104357,
            whole genome shotgun sequence.
ACCESSION   DATASH010000929 DATASH010000000
VERSION     DATASH010000929.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 11974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11974
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5104357"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..5118
                     /locus_tag="VHV99_24855"
     CDS             <1..5118
                     /locus_tag="VHV99_24855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018432369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HEX3638651.1"
                     /translation="GLHSPESDLRYADPQPALFSFNSAYGACEVCRGFGRVIGVDLGL
                     VIPDARKTLREGAIKPMQTPAWKECQDDLMRYAAKADIRRGTPWGELTDAERDWVING
                     SPDLNGKWQSHWYGVKRFFEYLESKAYKMHIRVLLSKYRSYTPCETCGGARLKTESLL
                     WRLGSKQNADEVLAPSQRFMPRGVEWSRAQLESLPGLTVHDLMLMPIERIRRFFDEIS
                     LPSALLDDALKLLLAEVRTRLKYLCDVGLGYLTLDRQSRTLSGGEVQRINLTTALGTS
                     LTKTLFVLDEPSIGLHPRDLNRIVEAMHRLRDAGNTLVVVEHDPSVMLAADRLIDMGP
                     GPGERGGSIIYDGAPEAIRSSGTLTGEYLGGRRHVADAAHWSQRPVDAGTPRIVLEGA
                     TEHNLQDVSVEIPLERLVCVTGVSGSGKSTLLQDVLYPAMARHFGLATESPGAFRSLT
                     GADQVTDVVFVDQSPIGKTARSNPASYVGAFDEIRKLFAKAPLAQQRGYGPGMFSFNS
                     GDGRCPTCGGSGFEHIEMQFLSDVYLRCPDCDGRRYRAELLEVKIERGEPVRALSIAD
                     VLELTVSEAIAYFAKDAEVLRVLQPIVDVGLEYVKLGQPVPTLSGGEAQRLKLAGFLA
                     ESAQARTARSAKQPVAKRLFMFDEPTTGLHFDDIAKLMQAFGKLLASGHSLIVIEHNL
                     DVVRAADWLIDLGPEGGDGGGRVLCAGTPEEVKLCPESHTGEALLQYDRAMNAAAEPA
                     SQGIPLQKALSAARARRAIEGEDVVRIVNAREHNLKALDVDIPHGKFNVITGVSGSGK
                     STLAFDILFHEGQRRYLESLNAYARSIVQPAGRPEVDAVYGIPPTVAIEQRLSRGGRK
                     STVATTSEVWHFLRLLYVKLGLQHCIHDGTPVTSQSVESIAAQLLRDHKGQHVGFLAP
                     LVVNRKGVYTDLAKWAKARGNTHLRVDGEFVPVDPWPKLDRFREHTIELPVTDLVVSP
                     DNEAELRQALDQTLEIGKGVMHLLAPLDGLHDAISGGRATAKLGTVKVLSTKRACPVC
                     GTSYPELDPRMFSYNSKHGWCTTCVGTGLALTREQRAAYDDTVMVEDNRGREQSLPSE
                     EQEPEGLVDEPCADCSGTRLNPTARAVFFGEQAIVDVAQWTVSDTRAWIDTLEMSGRD
                     AEIARDVVSEIGSRLQFLEEVGLGYLSLDRAAPSLSGGEAQRIRLAAQLGSNLQGVCY
                     VLDEPTIGLHPRDNQILLNALRKLGEKGNTLVVVEHDEDTIRRADHIIDIGPGAGKRG
                     GTLVAQGSVADLSAQPDSLTGQFLAHPIVHPLQPRREVVAPTKRATAVPENWLTVHGG
                     KLHNLRNVTVGIPLSRLVAVTGVSGSGKSTLARDVLMANLLDAVGRSVLSSPATRRAR
                     AAAETQPAANRRSSVLARTAPRAPLNVTHAWQGCDAITGWETIDRVLEVDQTPIGKTP
                     RSCPATYIGVWDAIRKLFAGTLEARARGYTASRFSFNTGEGRCPACEGQGVRTIGMSF
                     LPDVKVPCDVCHGQRFNPETLAVTWRGKNIGDVLTMEIDEAVEFFAPISNIAHPLQLM
                     KDVGLGYLTLGQPSPTLSGGEAQRIKLVTELSKVRDDITRRGQKPPHTLYVLDEPTVG
                     LHMADVAKLIRVLHRLADGGHSVVVIEHDLDVIAEADWIIDLGPEGGVGGGTIVAATD
                     PEGLLRVAASHTGAALRPVLQRAQSDAAMMAGEGTNG"
     gene            5241..6677
                     /locus_tag="VHV99_24860"
     CDS             5241..6677
                     /locus_tag="VHV99_24860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004532569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX3638652.1"
                     /translation="MIDERWAQAARLLTSRAAFWSLRIVDEQTDDHEIRNDIAQPMRT
                     VRDRGAMLIAWVGAGAGYAATANLSAAGLQAALNLATARAQASAAMSLIDHREVARPA
                     VSGHYVSPNAQHALPSRAEWLDRLGVECASANLDTRIVERVAAVQITHTDQLYITGDG
                     VRIDQQFQFVMPQLSVAAHANGDTQVRTLGGDYGTLGQGGMEVLTRFGFDGSGVRVAN
                     EALQLLGAPNCPSGKRDLLLMPDQMMLQIHESIGHPLELDRILGDERNFAGWSFVKKE
                     MFGSYRYGSALLNVTFDPELREEAAAYAFDDDGTEAKKHYLIRDGVLERPLGGALSQQ
                     RANMAGVANSRASSWNRPPIDRMANLNIEPGESSLEQMIGNIEHGILMRTNTSWSIDD
                     HRNKFQFGCEFGQLIENGKLTQIVKQPNYRGISANFWRSLSAVGNADTREVYGTSMCG
                     KGEPAQIIRVGHASPACVFSNIDVFGGA"
     gene            6677..8038
                     /locus_tag="VHV99_24865"
     CDS             6677..8038
                     /locus_tag="VHV99_24865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX3638653.1"
                     /translation="MSQFMSSRSGVLVDWQQHFRSLADAIERLQQGAETTLSSFAGEQ
                     SDFIRFNSGKVRQIGSVSQGKLTLRLIDGARQAYSTLTVCGDLQQDLDDVSAALATLR
                     ESLRGAADDPHLLFDTSAWQRTTQRSGKLPEPDSLLRTVAECAQGLDFVGFYAGGTIV
                     RGFASTSGSRGWYEVDNFNFSWSLYDPSGRAIKTTYAGDEWSEAVFAHKVEQAAARLP
                     VLARTPRTLAPGRYRSYLAPEALAELLSVTAWSGFSARAQASSRSELYKLHVGEIAVD
                     PRVTISEDLALGITPGFNDDGYLRDSVPLIQSGHSAERLTNARSAREYGLTPNGALAN
                     ESPAALSMQAGDLLEEDVLAKLDTGLYIGNLWYVNFSDRMNCRLTGMTRFATFWVENG
                     QIVAPLEAMRFDDSLYRLLGSELEQLGAHAEMLLSDSTWGERATGGMQLPGILTRSFE
                     LTL"
     gene            8094..8339
                     /locus_tag="VHV99_24870"
     CDS             8094..8339
                     /locus_tag="VHV99_24870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638654.1"
                     /translation="MTSDVARPGWFMVGAAAVLDPTSSHGVLKEILSGVTAAHLIAAV
                     LADKASADDAAHAYHDWLADWSTPMPRNWRTSIVSSA"
     gene            complement(8362..8706)
                     /locus_tag="VHV99_24875"
     CDS             complement(8362..8706)
                     /locus_tag="VHV99_24875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4148 domain-containing protein"
                     /protein_id="HEX3638655.1"
                     /translation="MKLATRTLVATLLLIGSASAMAAPGLTQQQCSSYPFKRLKGEVT
                     HSQLMQELGELEALGYDPDHDDDEYPSDLQQAQQKLQAEYRADCMPAANLTTSQNSGT
                     LAPSQASTTPAG"
     gene            9068..9745
                     /locus_tag="VHV99_24880"
     CDS             9068..9745
                     /locus_tag="VHV99_24880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015877212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3638656.1"
                     /translation="MSRVLTIEDDEITANEIVGELKSRGFTVDWVANGRDGMARALSE
                     DYDVITLDRMLPGVDGLTILTTMRSIGIQTPVLMLSALGDVDERVRGLRAGGDDYLTK
                     PFDPEEMTARLEVLLRRSQTPATPLETQLRVGPLELDLISRKVQRDGEEIALLPTEYR
                     VLEFMMRHVGQTITRTMLFEAVWGYHFDPGTNLIDVHMGRLRKKIDPLGVSPMIQTVR
                     GSGYILA"
     gene            9759..11156
                     /locus_tag="VHV99_24885"
     CDS             9759..11156
                     /locus_tag="VHV99_24885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX3638657.1"
                     /translation="MDPTFPAQSALGQRWHSTTFRLLSIYGVIFSLSVMVLLGYIGWA
                     VTGEMERETDVVMDWQLVYFDSVPDAGLADAIHRRMEHERMHTNYYGLFAADGRLIAG
                     DVLVYPSQLPTDRSGKTLDLVLSIARNDQVPVVRAMAERRNDGSTLVIARDLTHVLRI
                     RETIINALVGGGIVCLLAGVAGGLALSVRQMRRLKAIRRVTQRIAQGDLAQRLPMGGR
                     DEVDMLAHLVNHMLAEVERLMNEVKGACDGIAHDLRTPLAHVRTLLAHAAERTDTLDD
                     AALSQLVERARTETDALLDRFRAMLRISEIGTLQRRGGFGEMQLETLVQEVGELYEPL
                     AESRSIQLSVHVQPVDAIHGDRALLFEALSNLVDNAIKFTPEGGAVRMELRQTSSGPQ
                     VDIVDNGYGIAADERDAVLQRFYRGERTRHLAGSGLGLSIVSAVMRVHDFTMKIGNAQ
                     PGTRISIECWSRTLA"
     gene            11192..11905
                     /locus_tag="VHV99_24890"
     CDS             11192..11905
                     /locus_tag="VHV99_24890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012339832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3638658.1"
                     /translation="MRILFVGPSHPEAAWLFKALQESAHSLQRADDLRDGVFLASQEP
                     YDAVVLMVLEVGFYSSLQGFIGEFAAAGNGAAIVAVLGAATAQDRTRVLRAGADACFC
                     QPYSFIEMHERMAALQRVAGGRVAVGGGSGFVSAAASVLPAVGGACLDTATRELVFDG
                     RRVAVTRREFLLLECLLKQVNAPVARDQLIRYAWPEKEDVDPSSVNLVVSRLRRKLVG
                     DVPEVRIETVSRFGYQVSV"
BASE COUNT         2178 a         3530 c         3954 g         2312 t
ORIGIN      
        1 ggtctgcaca gccccgagag cgacctgcgc tatgcggatc cgcagccggc gctgttctcg
       61 ttcaattcgg cttacggcgc atgcgaggtc tgccgcgggt tcggccgcgt gattggcgtc
      121 gacctcggcc tcgtgatccc cgacgcgcgc aagacactgc gcgaaggcgc catcaagccg
      181 atgcaaacgc cggcctggaa agaatgccag gacgacctga tgcgctatgc ggcgaaagcc
      241 gatattcgcc gcggtacgcc ttggggcgag ttgacggacg ccgagcgcga ctgggtcatc
      301 aacggttcgc cggatttgaa cggcaagtgg caaagccatt ggtacggcgt caaacgcttc
      361 ttcgaatatc tcgaatcgaa agcgtacaag atgcacatcc gcgtgctgct gtcgaagtac
      421 cgcagctata cgccgtgtga gacctgcggc ggtgcgcgtt tgaaaaccga gtcgctgtta
      481 tggcgcctcg gtagcaagca gaatgcggac gaggtgctcg cgccctcgca gcgcttcatg
      541 ccgcgcggcg tcgagtggtc gcgtgcgcaa ctcgagtcct tgccgggttt gaccgtgcac
      601 gatctgatgc tgatgccgat cgagcgcatc cggcgtttct tcgacgagat cagcctgccg
      661 agcgcgctgc tcgacgacgc gctcaaacta ttgctcgccg aggtgcgtac gcgcctcaag
      721 tatctgtgcg acgtcgggct cggctatctg acgctcgacc ggcaaagccg cacgctctcg
      781 ggcggcgagg tgcagcggat caatctgacc accgcgctcg gcacatcgct gaccaaaacg
      841 ctgttcgtgc tcgacgagcc gagcatcggc ctgcatccac gcgatctgaa ccggatcgtc
      901 gaggccatgc accggctgcg cgacgcgggc aatacgctgg tggtggtcga acacgatccc
      961 tcggtgatgc tcgcggccga tcgcctgatc gatatgggcc cggggccggg cgagcgcggc
     1021 ggctcgatca tctacgacgg tgcgcctgag gcgatccgct cgtccggcac gctgaccggc
     1081 gagtacctgg gtggtcgccg gcatgtggcg gacgcggcgc actggtcgca gcgtccggtg
     1141 gatgccggca cgccacgcat cgtgctcgaa ggggcgaccg aacacaatct gcaagacgtc
     1201 agcgtcgaga ttccgttgga acggctcgtc tgcgtcacgg gggtgtccgg ctccggcaag
     1261 tccaccttgc tgcaggatgt gttgtatccg gccatggcgc ggcacttcgg cctcgccaca
     1321 gaatcacccg gcgcattcag aagcctgacc ggcgccgacc aggtcactga cgtggtattc
     1381 gtcgaccagt cgccgatcgg caagaccgcg cgctcgaatc cggccagcta cgtgggcgca
     1441 ttcgacgaaa tccgcaagct gttcgccaag gcgccactcg cgcagcaacg cggttacggc
     1501 cccggcatgt tcagcttcaa ctccggcgac gggcgctgcc cgacctgcgg cggctcgggc
     1561 ttcgaacaca tcgaaatgca gttcctgagc gacgtctacc tgcgttgccc ggattgcgat
     1621 gggcggcgct atcgagcgga actgctggaa gtgaaaatcg agcgcggcga acccgtacgc
     1681 gccttgagca ttgccgatgt actggaattg acggtaagcg aagccatcgc ctacttcgca
     1741 aaggatgccg aagtattacg cgtgttgcag ccaatcgtcg acgtcggtct cgaatacgtg
     1801 aagctcggtc agccggtgcc cacgttatcc ggcggcgagg cgcagcggct caagcttgcc
     1861 ggcttcctcg cggaatcggc gcaggcgcgc accgcgcgca gcgcgaagca accggtcgca
     1921 aaacgcctgt tcatgttcga cgaaccgacc accgggctgc atttcgacga catcgccaaa
     1981 ttgatgcagg cattcggcaa gctgctggca agcggccatt cgctgatcgt gatcgaacac
     2041 aatctcgacg tggtccgtgc ggccgactgg ctgatcgacc tcggccctga aggcggtgac
     2101 ggcggcggca gggtgctgtg cgcgggcacg ccggaagagg tcaagctctg tccggaatcg
     2161 cataccggcg aggcgctgct gcaatacgac cgcgccatga acgcagcggc cgaacctgcg
     2221 tcgcagggta ttccgctgca gaaggcactg agcgcggcgc gcgcgcggcg ggcgatcgaa
     2281 ggcgaggatg tggtgcgcat cgtcaatgcg cgcgagcaca atctgaaagc gctggatgtg
     2341 gacattccgc acggcaagtt caatgtgatc accggcgtgt ccggttcggg caagtccacg
     2401 ctggccttcg acattctgtt ccacgaaggg cagcgccgtt acctcgaatc gttgaacgcg
     2461 tacgcacgct ccatcgtgca gccggccggc cggcccgagg tggacgcggt gtatggcatt
     2521 ccccccaccg tcgcgatcga acagcgtttg tcgcgcggcg gccgcaagag tactgtggcg
     2581 accacgtccg aagtctggca cttcctgcgc ctgctctatg tgaagctcgg tctgcaacat
     2641 tgcatccacg acggcacgcc ggtcacgtcg caaagcgtcg aatcgattgc cgcgcaattg
     2701 ctgcgcgatc acaaagggca gcacgtcggt ttcctcgcgc cgctggtcgt caaccgcaaa
     2761 ggcgtctata ccgacctcgc caagtgggcg aaagcgcgcg gcaatacgca tctgcgtgtc
     2821 gacggtgaat tcgtgccggt ggacccgtgg ccgaagctcg atcgtttccg cgagcacacc
     2881 atcgagttgc cggtgaccga cctcgtcgtg tcgccggaca acgaagcgga actgcgccag
     2941 gcgctcgacc agacactgga aatcggcaag ggcgtcatgc atctgctggc gccgctcgac
     3001 ggtttgcacg acgcgatcag cggcggccgt gccacggcga aactcggcac ggtgaaggta
     3061 ctgtccacca agcgcgcctg tccggtctgc ggcacgagct atcccgaact cgacccacgc
     3121 atgttctcgt acaacagcaa gcacggctgg tgtacgacgt gcgtcggcac gggcctcgcg
     3181 ttgacgcgcg aacagcgcgc ggcatacgac gacaccgtga tggtcgaaga caaccgtggc
     3241 cgcgagcaga gcttgccttc cgaggaacag gaaccggaag gcctcgtcga cgaaccgtgt
     3301 gcggattgca gcggcacgcg cttgaaccct actgcacgcg ccgtgttctt cggcgagcaa
     3361 gccatcgtcg acgtcgcgca atggacggtg tccgacacgc gcgcgtggat cgacacattg
     3421 gagatgagcg gtcgagacgc ggaaatcgcg cgcgacgtgg tcagcgaaat cggcagccgc
     3481 ctgcaatttc tggaagaagt cggactcggt tatctgagcc tcgatcgcgc ggcgccgagc
     3541 ctctcgggcg gcgaggcgca gcgtatccgg cttgccgcgc aactgggcag caacctgcaa
     3601 ggcgtgtgct acgtgctgga cgagccgacc atcggcctgc atccgcgcga caaccagatt
     3661 ttgctcaacg ccctgcgcaa gctgggcgaa aagggcaaca cgctggtggt ggtcgagcat
     3721 gacgaggaca cgatccggcg tgctgatcac atcatcgata tcgggcccgg cgcgggcaag
     3781 cgcggcggca cgctggtcgc gcagggcagc gtggccgatc tgtcggcgca gcccgattcg
     3841 ctgaccgggc agtttctcgc ccatccgatc gtgcatccgt tacagccgcg ccgcgaggtc
     3901 gtcgcgccga ccaaacgcgc aaccgcggtg ccggagaact ggttgacagt gcacggcggc
     3961 aaactgcaca acctgcgcaa cgtcaccgtc ggcattccgc tttcacggct ggtggcggtg
     4021 accggcgtga gcggttcagg caaatcgacg cttgcgcgcg atgtgctgat ggccaacctg
     4081 ctggatgcag tggggcgctc ggtgctgtcg tcgccggcta cgcggcgcgc gcgcgcggcg
     4141 gccgagaccc agccggcggc gaaccggcgt tcaagcgtgt tggcgcgcac ggcgccgcgt
     4201 gcgccgctga acgtcacgca cgcctggcaa ggatgcgatg cgatcaccgg ttgggaaacc
     4261 atcgaccgtg tgctcgaagt cgatcagaca ccgatcggca aaacaccgcg ctcgtgtccg
     4321 gcaacttaca tcggcgtatg ggatgcgatc cgcaagctgt tcgccggcac gctcgaagcg
     4381 cgcgcgcgtg gctatacggc atcgcgtttc tcgttcaaca ccggcgaagg acggtgcccg
     4441 gcctgcgaag ggcagggcgt gcgtaccatc ggcatgagct tcctgcccga cgtgaaggtg
     4501 ccatgcgacg tttgccacgg tcagcgcttc aatccggaga cgctggcggt cacgtggcgc
     4561 ggcaagaaca tcggcgacgt gctgaccatg gagatcgacg aagccgtcga gttcttcgca
     4621 ccgatctcga acatcgcgca tccgttgcaa ttgatgaagg atgtcgggct cgggtatctc
     4681 acgcttgggc agccgtcgcc tacgctatcg ggtggcgaag cgcagcgtat caagctcgtc
     4741 acggagttga gcaaggtgcg cgacgacatc acgcggcgtg gtcagaagcc gcctcatacc
     4801 ttgtatgtgc tggacgaacc gactgtcggc ttgcatatgg cggacgtcgc gaaactgatt
     4861 cgcgtgctgc atcgtctcgc ggacggcggc catagcgtcg tggtcatcga gcacgatctc
     4921 gacgtgattg ccgaggcgga ctggatcatc gatctcggtc cggaaggggg tgtgggtggt
     4981 ggcacaatcg tcgccgctac cgaccccgaa ggcctgttac gcgttgccgc cagccacaca
     5041 ggggcggcgt tgcgtcccgt gctccagcga gcgcaaagcg atgcggcaat gatggcgggc
     5101 gagggcacta acggttgagg cagcgaagcg gcgcaataaa ctttgcgctg cttcgaatct
     5161 tgcaacaccc agtcgctgct ggcgtcgttg cgaatcgcgt cgcgccgcgg gcgccgcaga
     5221 gactcctacg agaatcgcta atgattgacg aacgttgggc gcaagccgcc cggttgctga
     5281 cgagccgcgc ggcgttctgg tcgctgcgca ttgtcgatga acagaccgac gatcacgaga
     5341 tccgcaacga catcgcgcaa cccatgcgca cggtacgcga tcgcggcgcg atgctgatcg
     5401 catgggtggg cgcgggagca ggctatgcgg ccaccgcgaa tctgagtgcc gctggcctgc
     5461 aggcggcgct gaatctcgcc acggcccgcg cgcaagcgag cgcggccatg tcgttgatcg
     5521 accatcgcga agtcgcgcgt cctgccgtga gcggccacta cgtatcgccg aatgcgcagc
     5581 atgcgctgcc gagccgcgcc gaatggttgg accgcctcgg cgtggaatgc gcgagcgcga
     5641 atctcgacac acggattgtc gaacgcgtcg cggccgtgca gattacgcat accgatcagc
     5701 tttacatcac cggtgacggc gtgcggatcg atcagcagtt ccagttcgtg atgccgcaat
     5761 tgagtgtggc cgcgcacgcg aacggcgata cgcaagtgcg tacccttggc ggcgactatg
     5821 gcaccttggg gcagggcggc atggaggtgc tgacgcgctt tggcttcgac ggctcgggtg
     5881 tgcgtgtcgc caatgaggcg ctgcaattgc tgggcgcgcc gaattgcccg tcgggcaagc
     5941 gcgatttgct gctgatgccc gatcagatga tgctgcagat tcacgaatcg attggccatc
     6001 cgctcgaact cgatcgtatt ctcggcgacg agcgcaactt cgccggctgg agcttcgtca
     6061 aaaaggaaat gttcggctcg taccgttacg gctcggcact gctgaacgtg accttcgacc
     6121 cggaactacg cgaagaagcc gcagcgtacg cattcgacga cgacggcacg gaagcgaaaa
     6181 agcactatct gatccgcgac ggcgtgctcg aacgtccgct cggcggcgcg ctttcgcagc
     6241 aacgcgcgaa tatggcgggc gtggcgaatt cacgcgcgtc gagctggaac cggccaccga
     6301 tcgaccggat ggcgaatctg aacatcgaac ccggcgagag ctcgctggaa cagatgatcg
     6361 gcaacatcga acacggtatt ctgatgcgca cgaatacgtc ctggtcgatc gacgaccatc
     6421 gcaacaaatt ccagttcggc tgcgagttcg gtcaactgat cgaaaacggc aagcttactc
     6481 agatcgtcaa gcaaccgaat taccgcggca tctcggcgaa tttctggcgc agcctgagcg
     6541 cggtgggcaa tgcggacacg cgtgaggttt acggcacgtc tatgtgcggc aagggtgagc
     6601 cggcgcagat tattcgcgtc ggccacgcat cgccggcttg cgtgttcagc aacatcgacg
     6661 tgttcggagg cgcatgatga gccaattcat gtcctcgcgt tctggcgtgt tggtcgattg
     6721 gcagcagcat ttcaggtcgc ttgccgatgc gatcgaacgc ttgcagcagg gcgcggaaac
     6781 cacgctgagt tcattcgccg gcgagcaatc cgactttatc cgcttcaact ctggcaaagt
     6841 gcggcagatc ggcagcgtgt cgcaaggcaa gctgaccttg cgtttgatcg acggcgcacg
     6901 gcaagcctat tccacattga ccgtctgcgg cgatctgcaa caggatctgg acgatgtgag
     6961 cgcggcgctc gccaccttgc gtgaaagcct gcgcggcgcg gcggacgatc cgcatctgtt
     7021 gttcgacacg tcggcatggc agcgcaccac ccaacgttcg ggcaagctgc cggagccgga
     7081 cagtctgctg cgcaccgttg ccgaatgcgc gcaaggactg gatttcgtgg gcttttacgc
     7141 cggcggcaca atcgtgcgcg gtttcgcgtc cacgagcggc agccgcggtt ggtacgaagt
     7201 cgataacttc aatttcagct ggtcgctata cgatccgagc ggccgcgcga tcaaaacgac
     7261 ttacgccggc gacgagtgga gcgaggctgt ttttgcccac aaggtcgagc aggcagcagc
     7321 gcgtctgccc gtgctcgcgc gtacgccgcg aacattggcg cctgggcgct atcgctcgta
     7381 tctggcgcct gaagcgctcg cggaattgct cagcgtgaca gcgtggagcg gtttctccgc
     7441 gcgcgcccag gcgagttcgc gcagcgagct atacaaactg catgtgggag aaatcgcagt
     7501 cgacccacgc gtcacgatca gcgaagatct ggcgctcggt attacacccg gcttcaacga
     7561 tgacggctac ctgcgcgaca gcgtgccgtt gatccagtcc ggccacagcg ccgaacgcct
     7621 gaccaatgca cgcagcgcgc gcgagtacgg tctgacgccc aacggtgcgc tggcgaacga
     7681 gtcgccggcg gcgctctcga tgcaagcggg cgatctgctt gaagaagacg tgctggcgaa
     7741 actcgatacg ggtctctaca tcggcaatct ctggtacgtg aatttctcgg accgaatgaa
     7801 ttgccgcctg accggcatga cgcgctttgc gaccttctgg gtcgagaacg ggcaaatcgt
     7861 tgcgccgctc gaagcaatgc gtttcgacga cagtttgtac cgtctgctcg gcagcgaact
     7921 cgaacaactc ggcgcgcatg cggaaatgct cttgagcgat tcgacatggg gcgagcgcgc
     7981 cacgggcggc atgcaattgc ctggcatcct gacgaggtcg ttcgaattga cgctgtgagc
     8041 ctgtgaatca agacgcgccg cgcgatgatt gagcaggaca accggaacgt tgcatgacga
     8101 gcgacgttgc gcggccgggc tggttcatgg ttggcgccgc cgccgtgctc gaccctacgt
     8161 cgtcacatgg cgtgctgaag gaaatcctgt cgggcgtcac ggcagcacat ctgattgccg
     8221 ccgtgctcgc cgacaaggcc tcggcggacg acgcggcgca cgcctatcac gactggcttg
     8281 ccgactggtc aacaccgatg ccgcgcaatt ggcgcacttc tatcgtgagc tcggcgtgac
     8341 cggttcggct agacgctcac actagccggc cggggtcgtc gatgcttgcg aaggcgcgag
     8401 ggtgcctgag ttttgcgagg tcgtcaggtt cgcggccggc atgcagtccg cgcgatattc
     8461 ggcttgcagt ttttgctgtg cctgttgcag atcgctgggg tattcgtcat cgtcgtggtc
     8521 ggggtcgtag ccgagcgctt ccagttcgcc gagttcttgc atcaactggc tgtgcgtgac
     8581 ttcgcctttg agtcgtttga acgggtagct gctgcattgc tgctgcgtga ggccgggcgc
     8641 ggccatcgcc gatgcgctgc cgatcagcag aagcgtggcg acgagtgttc tggttgcgag
     8701 tttcattttt ataccatcca cgataaagtt tgagtcgggc cgcaaggcaa gccgcgggat
     8761 ggcagtaggt tagttgagcc gtcctacagg agcggcgtcg ggacaatgaa atttgtttta
     8821 tgatgtcgtc gttcgttttc agcgcactta cggcgaaatt aaatatttct tcatgaagcg
     8881 gatatttatc cgccatacga gcgcgtgtag cctgctgaca ggcactgagg cgaagcggcg
     8941 acagccgtcg aatcgcacgc ccgtgctctg aacgcgcaag ccgaatatgg catcatgtcc
     9001 tcctactgat aaccggtcgc gtccgccgct tgcttcggta gagcggactg cggcgcatgc
     9061 aatggtcatg tcgcgagtgc tgacgatcga agatgatgaa attaccgcga atgaaattgt
     9121 cggggaactg aaaagccgcg gcttcaccgt ggattgggtg gccaacggcc gcgacggcat
     9181 ggcgcgcgcg ctcagcgaag attacgatgt gatcacgctc gaccgcatgc tgccaggcgt
     9241 ggacggtctc acgatcctta ccaccatgcg cagcatcggc attcaaacgc cggtgctgat
     9301 gttgagcgcg ctgggcgatg tggacgaacg ggtgcgcggc ctgcgcgccg gtggcgacga
     9361 ttatctgacc aagcctttcg atcccgaaga aatgaccgcg cgtctcgaag tgctgttgcg
     9421 ccgcagtcag acaccggcca caccactcga aacacagctg cgggtcggtc cgctcgaact
     9481 cgatctgatt tcccgcaagg ttcagcgcga tggcgaagag atcgccttgc tgcccaccga
     9541 atatcgtgtg cttgaattca tgatgcggca cgtgggacag accattacac gcaccatgct
     9601 gttcgaagca gtgtggggtt accacttcga cccgggcacc aatctgatcg atgtgcacat
     9661 gggccggctg cgcaaaaaaa tcgacccgct cggcgtctcg ccgatgattc agaccgtgcg
     9721 tgggtcgggt tatatcctcg catgagcagg gcactttgat ggatccgacc ttcccagctc
     9781 agtctgcgct cgggcagcgc tggcattcca ccacgtttcg cctgctatcg atctacgggg
     9841 ttatcttttc gctttcggtg atggtgttgc tgggctatat cggttgggcc gtgaccggcg
     9901 aaatggagcg cgagaccgat gtggtgatgg actggcaact ggtctacttc gattcagtgc
     9961 ctgacgcagg ccttgccgac gcgattcatc ggcgtatgga acacgagcgc atgcacacca
    10021 attactacgg tctgttcgcc gcggacggcc gtttgatagc cggtgacgtg ctggtgtatc
    10081 catcgcaact gcccactgat cgctcaggca aaacgctcga tctcgtgctt tcgatagcgc
    10141 gcaacgacca ggtgccggtg gttcgcgcca tggccgagcg ccgcaatgac ggctcgacgc
    10201 ttgtcatcgc gcgtgatctg actcatgtcc tgcggattcg cgagaccatc atcaacgcac
    10261 tggtgggcgg cgggatcgtg tgcctgctgg cgggcgtggc gggcggcttg gcgctgagcg
    10321 tgcggcagat gcgccgactg aaagcgatcc ggcgggtcac gcagcgtatt gcgcagggcg
    10381 atctcgcgca gcgcttgccg atgggcggac gcgacgaagt ggatatgctc gcgcatctgg
    10441 tgaaccatat gctcgcggaa gtcgaacgcc tcatgaatga agtgaagggc gcctgcgatg
    10501 gcattgcgca cgatctgcgc acgccgctcg cgcatgtgcg aaccttgctg gcgcatgcgg
    10561 cggagcgcac cgatacgctc gacgacgccg cgctgtcgca actcgtggag cgcgcgcgca
    10621 ccgagaccga tgcattgctc gaccggtttc gcgcgatgtt gcgtatctcc gagatcggca
    10681 cgctgcagcg gcgcggcggt ttcggcgaaa tgcagttgga gacgctggtg caggaagttg
    10741 gtgaactcta cgaaccactc gccgagagcc gctcgattca gctgtccgtg catgtgcaac
    10801 cggtcgatgc gattcatggc gaccgtgcgt tgctgttcga agccttgagc aatctggtgg
    10861 ataacgcgat caagttcacg cctgaaggcg gggcggtgcg catggaattg cgccagactt
    10921 cgtcggggcc acaggtggat attgtcgata acgggtacgg cattgccgcg gatgagcgcg
    10981 atgcggtcct gcagcggttt tatcgtgggg aacgcacccg gcacctggct ggatcggggt
    11041 tgggtttgag catcgtttca gcggtgatgc gggtgcatga cttcacgatg aagatcggga
    11101 atgcgcaacc tggcacccgg atttccatcg aatgctggtc gaggacgctc gcatgaggtc
    11161 ttgttcttat cttgtcatgg cagggtggtg aatgcgcatc ctgtttgttg ggccttctca
    11221 tccggaagct gcgtggctgt tcaaggcgct gcaagagagc gcgcatagtc tgcaacgagc
    11281 cgacgatctg cgggatgggg tttttcttgc ttcgcaggaa ccgtacgatg cggttgtttt
    11341 gatggtgctt gaggttgggt tttattcttc cttgcagggg tttattggag agttcgctgc
    11401 ggcggggaat ggagcggcga ttgttgccgt gcttggggcg gctaccgcgc aggatcggac
    11461 aagggttttg cgcgccggcg cggatgcgtg tttttgccag ccttattcgt ttattgaaat
    11521 gcatgagagg atggccgctt tgcagcgggt tgcgggtggg cgagtggccg ttggcggtgg
    11581 ttccgggttt gtttctgctg cggcttctgt tttgcccgct gttggcgggg cttgcctgga
    11641 taccgctacg cgggaactcg tttttgacgg gcggcgggtt gccgttacgc ggcgggaatt
    11701 tctcttgctt gagtgcctgc tgaagcaggt caatgcaccg gtggcaaggg atcagctgat
    11761 ccgttatgcc tggccggaga aggaggatgt tgatccttct agtgtcaatc ttgttgtgtc
    11821 caggttgcgg cgcaagcttg taggggatgt gcctgaagtg cggattgaga cggttagtcg
    11881 gtttgggtat caggtttctg tataaagggt ttttcttgcg gggcgctttt gggttgtttt
    11941 tgcttagggt gttggccttt ccttgtgctg gttg
//
LOCUS       DATASH010000930         5737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_749821,
            whole genome shotgun sequence.
ACCESSION   DATASH010000930 DATASH010000000
VERSION     DATASH010000930.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5737
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749821"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VHV99_24895"
     CDS             complement(<1..358)
                     /locus_tag="VHV99_24895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX3638659.1"
                     /translation="MEFLDLGPFSRASGTVRLPGSKSISNRVLLLAALAEGETTITNL
                     LDSDDTRVMLAALEKLGVRLTREGDTCVVTGTRGAFTARTADLFLGNAGTAVRPLTAA
                     LAVNGGDYRIHGVPRMH"
     gene            complement(377..1318)
                     /locus_tag="VHV99_24900"
     CDS             complement(377..1318)
                     /locus_tag="VHV99_24900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEX3638660.1"
                     /translation="MTDVAAFSFNKLVIFGVGLIGGSLARALRERGETAGARAVMGVG
                     RSSASTARALELGVIDGAAALNDDAALRDALAGADFVLLAAPVAQTQPLLERIAPFLD
                     ASTIVTDAGSTKSDVVAAARAALGARVGQFVPGHPIAGREASGPDAALPDLYVNRNVV
                     LCPLPENAPEAVERVAAMWRATGACVRDMTPEQHDRVLASVSHLPHVLSFALVEQILT
                     SPDAALKLSFAAGGFRDFTRIAASSPEMWRDVCVANRVALLDELDTYTAVLARLRAAI
                     DAGDGTALEAVFARSRVARSEWQEQRTAGAALGDASK"
     gene            complement(1370..2482)
                     /gene="hisC"
                     /locus_tag="VHV99_24905"
     CDS             complement(1370..2482)
                     /gene="hisC"
                     /locus_tag="VHV99_24905"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX3638661.1"
                     /translation="MTTSFGPSYVRAIAPYIAGKPISEVAREFGLDEATIVKLASNEN
                     PLGMPESAQRAIAQAASELGRYPDANAFELKAALSERYGVPADWVTLGNGSNDILEIA
                     AHAFVEKGQSIVYAQYSFAVYALATQGLGARAIVVPAVKYGHDLDGMLAAITADTRLI
                     FVANPNNPTGTFIEGARLEAFLDKVPRHVVVVLDEAYTEYLPEEKRYDSIAWVRRYPN
                     LLVSRTFSKAFGLAGLRVGFAIAQPELTDLLNRLRQPFNVNTLAQAAAIAALNDKAFL
                     EKSAALNAQGYRLLTEGFDKLGLEYVPSDGNFVLVRVGNDDAAGNRVNLELLKQGVIV
                     RPVGNYGLPQWLRITIGLPAENEAFLAALEKTLATA"
     gene            complement(2564..3646)
                     /gene="pheA"
                     /locus_tag="VHV99_24910"
     CDS             complement(2564..3646)
                     /gene="pheA"
                     /locus_tag="VHV99_24910"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012328120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEX3638662.1"
                     /translation="MDDELNTRLKPLRERIDALDAQLIALLNQRAAVALEVGEVKKHF
                     NAPVFRPEREQQVIARLQDMSDGPLASEHISAIWREIMAASRALEKTIKAAYLGPVGT
                     YSEQAMHEYFGQSIEGLPCPSIDEVFRSVEAGAAEFGVVPVENSTEGAVSRTLDLLLQ
                     TQLTIGGELALPIHHNLLTLNGGLTGATRVCAHAQALAQCQRWLATNAPHLERQAVSS
                     NAEAARMAAEDPTVAAIAGDRAATHYGLQVAYALIQDDPHNRTRFVMIGKERTGVSGH
                     DQTSLIVSVANEPGAVFKLLEPLARHSVSMTRFESRPARVGTWEYYFYIDVEGHRDEP
                     AVAAALTELGEKAAFLKILGSYPRAR"
     gene            complement(3725..4807)
                     /gene="serC"
                     /locus_tag="VHV99_24915"
     CDS             complement(3725..4807)
                     /gene="serC"
                     /locus_tag="VHV99_24915"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006476577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HEX3638663.1"
                     /translation="MRVFNFSAGPAAMPEEVLRQAADEMLDWQGSGMSVMEMSHRGKE
                     FMSIHEEALVDLRELLAVPASHRILFLQGGGLGENAIVPMNLMGAKPRADFIVTGSWS
                     QKSLKEAQKYGAVHLAASGQTAEGFTRAPARSEWQLSDDPAYVHLCTNETIHGVETFE
                     IPDLGDIPLVADASSHILSRPMDIAKYGVLFGGAQKNIGMAGVTVVIVREDMLDRAQA
                     ICPSAFEWKTVAENNSMYNTPPTYAIYVAGLVFKWLKKQGGLIAMEARNLEKSKLLYD
                     AIDSSSFYLNKVERGSRSRMNVPFFLADESRNEDFLAGAKARGMVQLKGHKSVGGMRA
                     SIYNAVPLEGVKALVEYMKEFEQRSA"
     gene            complement(5165..>5737)
                     /locus_tag="VHV99_24920"
     CDS             complement(5165..>5737)
                     /locus_tag="VHV99_24920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HEX3638664.1"
                     /translation="AALVPTFAMAQALQSQAPAAAPAAAAAPVDPAKQAAIKDLLDAI
                     DAQKLVGAIGNSAQMQAKQLVPAILSDALSENKTMTDKQKQASVPTLQKNSVPKLVDG
                     AGQVFATDSFRQDAMQAQYDAYAKYYSTSEIKDLTAFYKSPTGRKFIQVQDQVGRDVV
                     NGLMQKYMPQSIKATRDQADKEVASVKPAK"
BASE COUNT         1072 a         1857 c         1751 g         1057 t
ORIGIN      
        1 catgcatacg cggcacgccg tggatgcgat agtcgccgcc attcaccgca agcgccgcgg
       61 tcagcggacg cacggccgtg ccggcgttgc caaggaacag atcggcggta cgcgccgtga
      121 acgcgccgcg cgtgccggtc accacacagg tgtcgccttc gcgggtcaag cgcacgccca
      181 gcttttcgag cgcggcgagc atcacgcggg tgtcgtcgga gtcgagcagg ttggtgatcg
      241 tcgtttcgcc ttcagcgagc gcggcgagca gcagcacacg attcgaaatg cttttcgaac
      301 cgggcaaacg aaccgtgcca gacgcacggg aaaagggtcc gagatcgagg aattccatga
      361 ttgagtccaa ttccggttat ttcgaagcgt cgccgagggc cgccccggca gtgcgctgtt
      421 cctgccattc gctacgcgca acgcgcgagc gcgcgaacac ggcttcgagc gcggtgccgt
      481 cgccggcgtc gatcgcagcg cgcaagcgcg cgagcaccgc cgtgtaggtg tccagttcgt
      541 cgagcagcgc aacccgattg gccacgcaca cgtcacgcca catttccggg ctcgatgcgg
      601 cgatccgcgt gaagtcacga aagccgccgg cggcgaatga caatttcaac gcggcatccg
      661 gcgaagtgag aatctgctcg accagcgcga aagacaaaac atgtggcaaa tggctgaccg
      721 acgcgagcac gcggtcatgc tgctcgggcg tcatgtcccg cacacacgcg ccggtcgccc
      781 gccacatcgc ggccacgcgc tcgaccgctt ccggggcgtt ctccggcagc ggacacaaca
      841 cgacgttgcg gttcacgtac agatcgggca acgccgcatc cgggccgctc gcctcacggc
      901 cggcaatcgg atgccccggc acgaactggc cgacgcgcgc accgagcgcc gcgcgtgccg
      961 cagcgaccac gtcggacttg gtgctgccgg catcggtgac gatcgtgctt gcgtcgagaa
     1021 acggcgcaat gcgttcgagc agcggctgcg tctgcgcgac aggtgcggcg agcagcacga
     1081 agtcggcgcc ggcgagcgca tcgcgcaacg cggcgtcgtc attcaacgcc gcggctccgt
     1141 cgatcacgcc caactccaac gcccgcgccg tcgacgcgga cgaccgcccg acacccatca
     1201 ccgcacgcgc gccagccgtc tcgccccgct cgcgcagcgc gcgcgcaagc gagccgccga
     1261 tcaggccgac accgaaaatc accagcttgt taaaagagaa cgctgccacg tcggtcacag
     1321 aataagcgcg ccttccatca agtgaagcgc gcgggtcgag gtcaaccgct tacgcggtgg
     1381 caagcgtctt ttcgagcgcc gcgaggaacg cttcgttttc tgccggcagg ccgatcgtga
     1441 tgcgcagcca ttgcggcaaa ccgtagttgc caaccgggcg cacgatcacg ccctgtttca
     1501 gcagttccag gttgacccgg ttgccggctg cgtcgtcgtt gccaacgcgc accagcacga
     1561 aattgccgtc cgacggcaca tactcgaggc cgagcttgtc gaacccttcg gtcaacaggc
     1621 gatagccttg cgcgttcaag gccgcgcttt tttccagaaa cgccttgtcg ttcagtgccg
     1681 cgatcgccgc ggcttgcgcg agcgtgttca cgttgaatgg ctgacgcaga cggttcagca
     1741 gatcggtcaa ctccggctgc gcaatcgcga agcccacgcg cagaccggcg aggccaaacg
     1801 ctttcgagaa cgtgcgtgac accagcagat tcggataacg gcgcacccac gcgatcgagt
     1861 cgtagcgctt ttcttccggc agatattccg tatacgcctc gtcgagcacg acgaccacgt
     1921 gacgcggcac cttgtcgaga aacgcctcga gcctcgcgcc ttcgatgaac gtgccggtcg
     1981 gattattcgg gttcgcgacg aagatcagac gggtgtctgc agtgatcgcg gcgagcatcc
     2041 cgtcgagatc gtggccgtat ttgacggccg gcacgacgat cgcacgcgca cccaagcctt
     2101 gcgtggcaag cgcgtacacc gcgaatgaat attgcgcgta gacgatcgac tgccccttct
     2161 cgacgaacgc atgcgcggca atttcgagaa tgtcgttgct gccgttgccg agcgtgaccc
     2221 agtcggccgg cacgccgtag cgctcgctca acgcagcctt cagctcgaat gcattcgcgt
     2281 ccggatagcg gccgagttcg ctggccgctt gcgcgatggc ccgctgtgcc gattccggca
     2341 tgccaagcgg attttcattc gacgcgagct tcacgatggt cgcttcgtcg aggccgaact
     2401 cgcgcgctac ttccgaaatc ggcttgccgg cgatgtaagg cgcaatcgcg cgcacatagg
     2461 aaggaccgaa ggacgttgtc atgaaaatct ccgaacgact aattggtagt gaatgaacat
     2521 gcggcaggcg cgcccgggtt gcatgagcgc gcctcgacag acgttagcgg gcgcgcggat
     2581 acgagccaag tatcttcagg aacgcggcct tctcgccgag ttcggtgagc gctgcggcga
     2641 ccgccggctc gtcgcgatgg ccttcgacgt cgatatagaa gtaatactcc cacgtgccga
     2701 cgcgcgccgg gcgcgactcg aaacgggtca tggagacgct atggcgcgcc aaaggctcca
     2761 gcaacttgaa cacggcgccc ggctcgttcg ccactgaaac gatcaacgac gtctggtcgt
     2821 gcccgctaac gccggtgcgc tccttgccga tcatcacgaa acgcgtgcgg ttgtgcggat
     2881 cgtcctggat cagcgcgtag gcaacctgca gaccgtaatg cgtcgcggcg cgatcgccgg
     2941 caatggcggc gacagttgga tcttccgccg ccatgcgcgc ggcttcggca ttgctcgaca
     3001 ccgcctggcg ctcgagatgc ggcgcgttgg ttgcgagcca gcgctggcat tgggcgagcg
     3061 cctgcgcatg tgcgcacacg cgcgtcgcgc cggtgaggcc accgttcagc gtcagtagat
     3121 tgtggtgaat cggcaacgcc agttcgccgc caatcgtgag ctgggtttgc agcaacaaat
     3181 ccagcgtgcg cgacaccgcg ccttcggtcg aattttcgac cggcacaacg ccgaattcgg
     3241 cggcgcccgc ttcgaccgag cgaaacactt cgtcgatcga cgggcacggc agaccttcga
     3301 tcgactgacc gaagtattcg tgcatcgcct gttcgctata ggtgccgacc ggaccgagat
     3361 acgcggcctt gatggtcttc tcgagcgcgc ggctcgcggc catgatctcg cgccagatcg
     3421 cgctgatatg ctcgctggcc agcggcccgt cgctcatgtc ctgcaggcgg gcgatcacct
     3481 gctgctcgcg ctcggggcga aacaccggcg cgttgaaatg ctttttgacc tcgcccactt
     3541 caagcgccac tgcggcgcgc tgattgagga gagcgatcaa ctgcgcgtcg agcgcgtcga
     3601 tgcgttcgcg aaggggcttg agtcgggtat tgagttcgtc gtccatgcgt gaaaacggcc
     3661 ctgccggtag ctgctgcaca aatgcgtgct ggaaaggata cgcgtgcctg aatgtccgcg
     3721 acaatcaggc gctgcgctgt tcgaattcct tcatgtattc gacaagcgct ttgacgcctt
     3781 cgagcgggac ggcgttataa atcgacgccc gcatgccgcc gacggacttg tggcccttta
     3841 gctgcaccat tccccgcgct ttcgcgccgg ccaggaaatc ttcgttgcgc gactcgtcgg
     3901 cgaggaaaaa cggtacgttc atccgcgagc gcgagccacg ctcgaccttg ttcagataga
     3961 agctgctcga atcgatcgcg tcgtacagga gcttcgattt ttcgaggttt cgcgcttcca
     4021 tcgcgataag gccgccctgc ttcttcaacc acttgaacac caaccccgcg acgtaaatcg
     4081 cgtacgtggg cggcgtgttg tacatcgaat tgttttcggc aacggttttc cattcgaacg
     4141 ccgacgggca gatcgcctgc gcgcgatcca gcatgtcttc gcgcacgatc acgaccgtca
     4201 cgcccgccat gccgatgttc ttctgcgcgc cgccaaacag cacaccgtat ttggcgatgt
     4261 ccatggggcg cgacagaata tgcgacgacg cgtccgctac cagcggaata tcgccgagat
     4321 cggggatctc gaacgtctcg acaccgtgaa tcgtttcgtt cgtgcacaaa tgcacgtaag
     4381 ccggatcgtc cgacagttgc cactccgagc gtgccggcgc gcgcgtgaag ccctcagccg
     4441 tctggccgct cgccgccaga tgcacggcgc cgtacttctg cgcttctttt agcgacttct
     4501 gcgaccacga acccgtcaca atgaaatccg cgcgcggctt cgcgcccatc agattcatcg
     4561 gcacgatcgc gttttcaccc aagccgccgc cttgcagaaa caggatgcga tgactggcgg
     4621 gcaccgcgag caactcgcgc aggtcgacga gcgcttcctc gtggatcgac atgaattctt
     4681 tgccgcgatg gctcatttcc atcacgctca tgccgctgcc ctgccaatcg agcatctcgt
     4741 cggccgcctg acgcagcact tcttcgggca tggccgctgg gccggcggag aaattaaaaa
     4801 cgcgcatcgt gagatcctgg aagctggcgc aaaaaacgga cagaacgcgc aaaaatcggc
     4861 gaagtctgca gccgctaagg cagccgttgc agcgacagcc gctgcaaagt tagcgacgcg
     4921 agttcggtga cgcgagttta acgacgcgcg ttgacaacga tttcgcgccc aaaaagaaaa
     4981 tggccgtctg cgcgtgtgcg caaacggcca ttatcgcatt gtcgccacca acccgccaaa
     5041 ccccgcatag cgtatagctt gccgagaagc ggctagcgcg gtgtcagcgc agcctttcga
     5101 cctgttcagc ccgataagtc ggctcagcga gtaggcccaa tgagcttgct cacttcgtca
     5161 aaacttactt agccggcttg accgaagcga cttccttgtc agcctggtca cgcgttgcct
     5221 tgatcgattg cggcatgtac ttttgcatca agccattcac gacgtcgcgg ccaacctggt
     5281 cctggacctg gatgaacttg cggccggtcg ggctcttgta gaacgcggtc agatccttga
     5341 tttccgacgt gctgtagtac tttgcatacg cgtcgtactg agcttgcatt gcgtcctggc
     5401 ggaagctgtc cgttgcgaac acctgacccg caccgtcaac cagcttcggc accgagttct
     5461 tttgcagcgt cgggacggaa gcctgctttt gcttgtccgt catcgtcttg ttttcgctca
     5521 gggcgtccga caggatggcc ggaaccagct gcttagcttg catttgcgcg ctgttgccga
     5581 ttgcaccgac gagcttctgt gcgtcgattg cgtccagcag gtccttgatt gcagcctgct
     5641 tggccggatc aaccggtgcc gcagcagcag ccggagccgc cgccggggct tggctctgca
     5701 gcgcttgagc catagcgaag gtcggcacca gagcagc
//
LOCUS       DATASH010000931         6740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1544265,
            whole genome shotgun sequence.
ACCESSION   DATASH010000931 DATASH010000000
VERSION     DATASH010000931.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6740
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1544265"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3967
                     /locus_tag="VHV99_24925"
     CDS             <1..3967
                     /locus_tag="VHV99_24925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HEX3638665.1"
                     /translation="FPAWELAHPYRMIAHNGEINTVKGNVNWLNARTGAIASHVLGDD
                     LPKLWPLIYPGQSDTASFDNCLELLVMAGYPLVHAMMMMIPEAWEQHTLMDDNRRAFY
                     EYHAAMMEPWDGPAAIAFTDGRQIGATLDRNGLRPARYIVTDDDLVIMASEAGTLTIP
                     ESKIVKKWRLQPGKMFLIDMEHGRIIDDKELKDNLANAKPYKSWIDAVRIKLDEIEPK
                     AEDVATERREPAALLDRQQAFGYTQEDLKFLMAPMAQAGEEAVGSMGNDSPLAVMSNK
                     NKTLYHYFKQLFAQVTNPPIDPIRENMVMSLVSFVGPKPNLLDTNNINPPMRLEVSQP
                     VLDFKDIAKIRAIDQYTGGKFSSYELNICYPVAWGKEGIEARLASLCAEAVDAVRSGY
                     NMLIVSDRKTDRDNVAIPALLATAAIHTHLVQHGLRTSAGLVVETGSARETHHFALLA
                     GYGAEAVHPYLAMETLGQLAAGLKGDLSPEKAVYNFTKAVGKGLMKVMSKMGISTYMS
                     YTGAQIFEAVGLAEDLVQKYFKGTSSKVSGIGLFDVAEEAIRLHRDAFGDNPVLANML
                     DTGGEYAYRVRGEEHMWTPDAIAKLQHSARSNSYQTYKEYAHLINDQTKRHMTFRGLF
                     EFKFDPTKAIPLDEVESAKDIVKRFATGAMSLGSISTEAHATLAVAMNRIGGKSNTGE
                     GGEDVNRYRNELRGIPIKNGDTMKSVIGDEVIVDIPLKDGDSLRSKIKQVASGRFGVT
                     AEYLASADQIQIKMAQGAKPGEGGQLPGHKVSEYIGKLRYSVPGVGLISPPPHHDIYS
                     IEDLAQLIHDLKNANSAASVSVKLVSESGVGTVAAGVAKAKADHVVIAGHDGGTGASP
                     LSSVKHAGTPWELGLAETQQTLVLNQLRGRIRVQADGQMKTGRDVVIGALLGADEFGF
                     ATAPLVVEGCIMMRKCHLNTCPVGVATQDPVLRAKFQGQPEHVVNFFFFVAEEAREIM
                     AQLGIRKFDDLIGHAELLDMKKGVEHWKAKGLDFSRVFYQPQVPAEVARKHVDAQDHG
                     LEKALDHVLIEKAKAAIEKGEHVSFIQPVRNVNRTVGAMLSGTIAKKYGHDGLPDDTI
                     HIQLKGTAGQSFGAFLARGITLDLVGDGNDYVGKGLSGGRIIIRPTNDFRGKSEENII
                     CGNTVMYGAIEGEAFFRGVAGERFCVRNSGATAVVEGTGDNGCEYMTGGTVIVLGETG
                     RNFAAGMSGGIAYVFDPDSTFAAKCNKSMVALDPVLQQAEQERTVDKSLWHTGTTDEA
                     LLKGLIESHFQFTGSPRAKALLENWDASRRQFVKVFPTEYKRALGEMAAKKANKEVLA
                     A"
     gene            4081..5550
                     /locus_tag="VHV99_24930"
     CDS             4081..5550
                     /locus_tag="VHV99_24930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016346426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HEX3638666.1"
                     /translation="MGKATGFLEFERRHEAYEAPLTRVKHYKEFVAALTDDEAKIQGA
                     RCMDCGIPFCNNGCPVNNIIPDFNDLVFHQDWKNAIEVLHSTNNFPEFTGRICPAPCE
                     AACTLGINDDAVGIKSIEHAIIDKAWAQGWVAPLPPKHKTGKKVAVVGSGPAGLAVAQ
                     QLARAGHDVTVFEKNDRIGGLLRYGIPDFKLEKWLIDRRMRQMEAEGVTFRANVFIGR
                     ADSLPEYIGNTAKETITPAELKEQFDAVVIAGGSETPRDLPVPGRELEGIHYAMEFLP
                     QQNKVNAGDKVANQLLAKGKHVVVIGGGDTGSDCVGTSNRHGAKSVTQFELLPQPPEE
                     ENKPLVWPYWPIKLRTSSSHDEGCERDWAVATKRLEGKNGKVEKLIAARVEWKDGKMQ
                     EVPNSEFEMKADLVLLAMGSTQPVSPVLEAFGVDKDARGNVRAATEGDKAYYTSVDKV
                     FTAGDMRRGQSLVVWAIREGRQCARSVDAFLMGHSELPR"
     gene            complement(5913..>6740)
                     /locus_tag="VHV99_24935"
     CDS             complement(5913..>6740)
                     /locus_tag="VHV99_24935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006052253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3638667.1"
                     /translation="ELAQPALAVLRDAGIVHREVSAAQCVEIEPGLRWARQTPLSGLY
                     LPDDEAGDCARFTRELRAICERHGVRFRFDTRVAGLDVERNAVRGVRIESERGSETVQ
                     ADAVVVALGVDSAALLAPLGVKVPLYPVKGYSATLPVTDEEKAPRAALMDESLKTAIT
                     RFGNNLRVAGTAELGNRQTTLREQALQTLMKVLGDWFPHAANPTSAHFWVGRRPMTPD
                     GAPLLGPSGVDKLWLNVGHGSTGWAMSMGSGRVVADLITQRKPEIDLDGLTLNRYRK"
BASE COUNT         1398 a         2051 c         2146 g         1145 t
ORIGIN      
        1 gttcccggcg tgggaactgg ctcacccgta ccggatgatc gcccacaacg gcgaaatcaa
       61 cacggtgaag ggcaatgtca actggctgaa cgcccgcacc ggcgcgatcg cgagccacgt
      121 gctcggcgac gacctgccga aactgtggcc gctgatctat ccgggccaat cggacaccgc
      181 gtcgttcgac aactgtctcg aactgctggt gatggccggc tacccgctcg tccacgccat
      241 gatgatgatg atcccggaag cctgggagca gcacacgctg atggacgaca accgccgcgc
      301 gttctacgaa taccacgccg cgatgatgga gccgtgggac ggccccgccg cgatcgcctt
      361 caccgacggc cgccagatcg gcgcgacgct cgaccgtaac ggtctgcgtc cggcgcgcta
      421 catcgtcacc gacgacgacc tggtcatcat ggcgtcggaa gcgggcacgt tgacgatccc
      481 cgagtcgaag atcgtcaaga agtggcgtct gcagccgggc aagatgttcc tgatcgacat
      541 ggaacacggc cgcatcatcg acgacaagga actgaaggac aacctcgcga acgccaaacc
      601 gtacaagagc tggatcgacg ccgtgcgcat caagctcgac gaaatcgagc cgaaggccga
      661 agacgtcgcg acggaacgcc gcgaacccgc tgccttgctg gatcgccagc aggcgttcgg
      721 ctacacgcag gaagacctca agttcctgat ggcgccgatg gcgcaagccg gcgaagaagc
      781 cgtcggttcg atgggcaacg actcgccgct ggcggtcatg tccaacaaga acaagacgct
      841 ctatcactac ttcaagcagc tgttcgcgca agtcacgaac ccgccgatcg acccgatccg
      901 tgaaaacatg gtgatgtcgc tggtgtcgtt cgtcggtccg aagccgaacc tgctcgacac
      961 gaacaacatc aacccgccga tgcgtctcga agtatcgcag ccggtgctcg acttcaagga
     1021 catcgcgaag atccgcgcga tcgatcagta cacgggcggc aagttcagct cgtatgaact
     1081 gaacatctgc tatccggtgg cgtggggcaa ggaaggcatc gaagcgcgcc tggcctcgct
     1141 gtgcgcggaa gccgtcgatg cggtcaggtc cggctacaac atgctgatcg tgtcggatcg
     1201 caagaccgac cgtgacaacg tcgcgattcc ggcattgctg gccaccgccg ccatccacac
     1261 gcatctcgtg cagcacggtc tgcgcacgag cgcgggtctc gtcgtggaaa ccggctcggc
     1321 gcgtgaaacg caccacttcg cgttgctcgc gggctacggc gcggaagccg tgcacccgta
     1381 cctcgcgatg gaaacgctcg gccagctcgc agcgggtctg aagggcgacc tctcgccgga
     1441 aaaggcggtg tacaacttca ccaaggcagt cggcaagggc ctgatgaagg tcatgtcgaa
     1501 gatgggcatt tcgacctaca tgtcgtacac cggcgcgcag attttcgaag ccgtcggtct
     1561 cgctgaagat ctggtgcaga agtacttcaa gggcacctcg tcgaaggtta gcgggatcgg
     1621 cctgttcgac gtcgcggaag aagcgatccg tctgcatcgc gacgcgttcg gcgacaaccc
     1681 ggtgctcgcg aacatgctcg acacgggcgg cgagtacgcg taccgcgtgc gcggcgaaga
     1741 acatatgtgg acgccggatg cgatcgccaa gctgcaacac tcggcgcgca gcaactcgta
     1801 ccagacgtac aaggaatacg cgcatctgat caacgatcag accaagcgcc acatgacgtt
     1861 ccgcggcctg ttcgaattca agttcgatcc gaccaaggcg atcccgctcg acgaagtgga
     1921 atcggcgaag gacatcgtca agcgtttcgc caccggcgcg atgtcgctgg gctcgatctc
     1981 gaccgaagcc cacgccacgc tggccgttgc gatgaaccgc atcggcggca agtcgaacac
     2041 cggtgaaggc ggtgaggacg tgaaccgtta tcgcaacgaa ctgcgcggca ttccgatcaa
     2101 gaacggcgac accatgaagt cggtgatcgg cgatgaagtg atcgtcgaca ttccgctgaa
     2161 ggacggcgat tcgctgcgct cgaagatcaa gcaggtggcg tcgggccggt tcggcgtgac
     2221 ggcggaatac ctcgcgtcgg ctgatcagat ccagatcaag atggcgcaag gcgcgaagcc
     2281 gggcgaaggc ggtcagttgc cgggccacaa ggtgtcggaa tacatcggca agctgcgcta
     2341 ctcggtgccg ggcgttggcc tgatttcgcc gccgccgcac cacgacattt actcgatcga
     2401 agatctggcg cagctgatcc acgacctgaa gaacgccaac tcggcggcga gcgtctcggt
     2461 gaagctggtg tcggaatcgg gcgtcggtac ggttgcagcg ggtgttgcca aggcgaaggc
     2521 cgatcacgtc gtgatcgccg gccatgacgg tggcacgggc gcttcgccgt tatcgtcggt
     2581 gaagcatgcc ggcacgccgt gggaactggg tctggctgaa acgcagcaaa cgctggtgct
     2641 gaaccaactg cgcggccgta tccgcgtgca ggccgacggt cagatgaaga ccggccgcga
     2701 cgtcgtgatc ggcgcgctgc tcggcgcgga cgaattcggc ttcgcaacgg cgccgctcgt
     2761 cgtcgaaggc tgcatcatga tgcgcaagtg ccacctgaac acctgcccgg tcggcgtcgc
     2821 gacgcaagat ccggtgctgc gtgcgaagtt ccagggtcag ccggaacatg tcgtcaactt
     2881 cttcttcttc gttgcggaag aagcgcgcga aatcatggcg caactgggca tccgcaagtt
     2941 cgacgacctg atcggccacg ccgaactgct cgatatgaag aagggcgtcg aacattggaa
     3001 ggcgaagggt ctggacttct cgcgcgtgtt ctatcaaccg caagtgccgg ccgaagtggc
     3061 acgcaagcac gtcgacgcgc aggatcacgg cctcgagaag gcactggatc acgtgctgat
     3121 cgagaaagcg aaagccgcga tcgagaaggg cgagcacgtc tcgttcatcc agccggtgcg
     3181 caatgtgaac cgtacggtcg gcgcgatgct gtccggcacg atcgcgaaga aatacggcca
     3241 cgacggtctg ccggacgaca cgattcacat ccagttgaag ggcaccgcgg gtcagagctt
     3301 cggcgcgttc ctcgcccgag gcattacgct ggacctggtc ggcgacggta acgactacgt
     3361 cggcaagggc ctctcgggcg gtcgcatcat catccgtccg accaacgatt tccgcggcaa
     3421 gtcggaagag aacatcatct gcggcaacac ggtgatgtac ggcgcgatcg aaggcgaagc
     3481 gttcttccgc ggcgtcgccg gcgagcgttt ctgcgtgcgt aactccggtg cgacggcggt
     3541 tgtcgaaggc acgggcgaca acggttgcga atacatgacg ggcggcacgg tgatcgtgct
     3601 cggcgaaacg ggccgtaact tcgcggcggg tatgtcgggc ggtattgcct acgtgttcga
     3661 tccggacagc acgttcgcgg ccaagtgcaa caagtcgatg gtcgcgctcg atccggttct
     3721 gcaacaggcc gaacaggaac gcactgtcga caagagcttg tggcacacgg gcacgaccga
     3781 cgaagccctg ctcaagggtc tcatcgaaag tcacttccag ttcacgggtt cgccgcgtgc
     3841 aaaggcattg ctcgaaaact gggatgcgtc gcgccgtcaa ttcgtgaagg tgttcccgac
     3901 cgaatacaag cgcgcactgg gcgagatggc agcgaagaag gcgaacaagg aagtactcgc
     3961 cgcttaactg tccgcaaccg ccgacgtcac atttagccgt acccgccgcg cctcaccgtt
     4021 caatcggtcc gcgcggcggg tgaagatcac ccacctgata cagatagaga agagaaccac
     4081 atgggcaagg caaccggttt tctcgagttc gagcgccgcc acgaggcgta cgaagctccg
     4141 ctcacgcgtg tgaagcacta caaggaattc gtcgcggccc tgaccgacga cgaagcgaag
     4201 attcaaggcg cgcgttgcat ggactgcggc attccgttct gcaacaacgg ctgcccggtg
     4261 aacaacatca tcccggactt caacgacctg gtgttccatc aggactggaa gaacgcgatt
     4321 gaagtgctgc actccaccaa caacttcccc gagttcacgg gccgcatctg cccggcgccg
     4381 tgcgaagcgg catgtacgct cggcattaac gacgacgcag tcggcatcaa gtcgatcgaa
     4441 cacgcgatca tcgacaaagc gtgggcgcaa ggttgggtcg cgccgctgcc gccgaagcac
     4501 aagaccggca agaaggtcgc cgtggtcgga tcaggccccg ccggtttggc cgtcgcgcag
     4561 caactcgcgc gcgcggggca cgatgtcacc gtgttcgaaa agaacgaccg catcggcggt
     4621 ttgctgcgtt acggcattcc cgacttcaaa ctggagaagt ggttgatcga ccgccgcatg
     4681 cgccagatgg aagcggaagg cgtgacgttc cgcgccaacg tgttcatcgg ccgggcggat
     4741 tcgctgccgg agtacattgg caataccgcg aaggaaacca tcacgccggc cgaactgaaa
     4801 gaacagttcg acgcagtggt gattgctggc ggttcggaaa cgccgcgcga tttgccggtg
     4861 cccggccgtg agctggaagg catccactac gcgatggaat tcctgccgca gcagaacaag
     4921 gtcaacgccg gcgacaaggt cgcgaaccag ttgctcgcca agggcaagca tgtggtcgtg
     4981 atcggtggtg gcgatacggg ttcagactgc gtcggcacgt cgaatcgtca tggcgcgaag
     5041 agcgttacgc aattcgagct gctgccgcaa ccgccggaag aagagaacaa acctttggtt
     5101 tggccgtact ggccgatcaa gttgcgtacg tcgtcgtcgc atgatgaagg ctgcgagcgc
     5161 gattgggcgg tggcgaccaa gcgtctcgaa ggtaagaacg gcaaggtcga gaagctgatc
     5221 gccgcgcgtg tcgaatggaa ggacggcaag atgcaggaag tgccgaactc ggaattcgaa
     5281 atgaaggccg atctggtgct gctggccatg ggctccacgc agccggtgtc gcccgtgctc
     5341 gaggcattcg gtgtcgacaa ggacgcgcgt ggcaacgtgc gtgccgcgac tgaaggcgac
     5401 aaggcgtatt acacctcggt ggacaaggtg ttcacggcgg gcgatatgcg tcgtggccag
     5461 tcgctggtgg tgtgggcgat tcgcgaaggc cgtcagtgcg cacgttcggt cgacgccttc
     5521 ctgatgggtc attcggaact gccgcgttaa gtgtcttttg tcgaccagag ttggcgcttt
     5581 agcgcttact ctggtcccat gcagcgcggt cgaccagagt tggcgcttta gcgcttactc
     5641 tggtcccatg caaggcggtc gagcagactt agcgcgttac tccagtccca tgcaacacgg
     5701 ttgctgctgg tttccgcttt aaggcgtaga acccagctaa tgcttgcagg aagaggtgga
     5761 gacgacaagg cagtacataa gcggtacgcc aaagcggtaa aaaacaaagc cgggcacctg
     5821 ggagggtgac ccggcttttc cgtttctgcg acgcgcagcg cgtcccatca atgcgttgag
     5881 tcgcgagcga cgtcccgcga taggtcgcgc ggttatttcc gataccggtt caacgtgagt
     5941 ccgtccagat cgatttccgg tttgcgctgc gtaatcaaat ccgcgaccac ccggcccgaa
     6001 cccatcgaca tcgcccaacc cgtcgagcca tgtccgacgt tcagccaaag cttgtccacg
     6061 cctgacggac caagcaacgg cgcgccatcg ggcgtcatcg gacggcggcc cacccagaag
     6121 tgcgcggagg tgggattggc cgcgtgcgga aaccagtcgc cgaggacttt catcagcgtc
     6181 tgcaaggcct gttcgcgtaa cgtggtctgg cgattgccga gttccgccgt gcccgccacg
     6241 cgcaggttgt tgccgaagcg cgtgatcgcc gttttcagcg actcgtccat cagcgctgcg
     6301 cgcggggctt tttcttcatc ggtgacgggc agcgtcgccg aatagccttt gaccggatag
     6361 agcggtacct tcacgccgag tggcgcgagc agcgccgcac tgtcgacgcc cagcgccacg
     6421 accaccgcat ccgcttgcac cgtctcgctg ccacgctcgc tttcgatccg cacgcctcgc
     6481 accgcattgc gttctacatc gagccccgcc acgcgagtgt cgaagcgaaa ccgcacgcca
     6541 tggcgctcgc agatcgcacg caattcgcgg gtgaagcggg cgcaatcgcc ggcttcgtcg
     6601 tcggggagat agagacccga gagcggcgtc tgacgcgccc agcgcaaacc cggttcgatc
     6661 tcgacgcact gcgccgcgct cacttcacga tgcacgatcc ccgcatcgcg cagaacagct
     6721 aacgccggtt gcgcgagttc
//
LOCUS       DATASH010000932         2362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3125397,
            whole genome shotgun sequence.
ACCESSION   DATASH010000932 DATASH010000000
VERSION     DATASH010000932.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3125397"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1282
                     /locus_tag="VHV99_24940"
     CDS             <1..1282
                     /locus_tag="VHV99_24940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004537186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate-binding module family 20
                     domain-containing protein"
                     /protein_id="HEX3638668.1"
                     /translation="DSNIGGYHLVWSRDLFKFANALITAGDTTTPATVVNYLFNTLQQ
                     KTDCGAAEYDADGCAQGFSRVGRFPQNSWVSGWPYWQGTQMDEQAMPILLAWRLGSGV
                     YNALWPKIALTADYILRTGPWTYQDRWEEDSGYSPSTIAAEVAGLVAAARIAQDNGDS
                     ARAGSYLAAADYDAVLAMNVKDSDQAWFRYNFDGYGEHNDGSNFDGTGVARAWPIFTA
                     ERGMFSIASSGVGSSGTAYLNAVKSYATPEGFISEQIWTNTVTLPGNWQVVTPPEFTA
                     GAPTKSMAPLNWAMGEYISLLASIHAGRVVDIPSIVCARYSNCVIAAASGQAGVTISV
                     KAATQPGQYMYVTGSTDALGNWNTDLGLPVDPANYPVWSNAVNLPASSSVQYKYYRKN
                     ADGSVTWEKVSGGGNRTLNVPASGGVTLNDTVGW"
     gene            1815..>2362
                     /locus_tag="VHV99_24945"
     CDS             1815..>2362
                     /locus_tag="VHV99_24945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX3638669.1"
                     /translation="MLTLKSDLHGNHLLGALPAHEWQALTPHLELVQLRTGQLLCDSG
                     QRIHHVYFPTTAIISLLHSMEDGSSAEIAAIGREGMTGVPILTGGDTMPTRVQVQCPG
                     FAYRMSAAALREQFNRSDFLRRLMLLYMQALLTQVAQTAACNRHHSLSKQLCRWLLIE
                     IDRTPSNEFQVTQQMIADMLGVR"
BASE COUNT          500 a          686 c          724 g          452 t
ORIGIN      
        1 cgacagcaat attggcggct atcacctcgt gtggtcacgc gatctcttca agttcgcgaa
       61 tgcactgatc accgccggcg atacaacgac acccgccacc gttgtcaatt acctcttcaa
      121 tacgctgcaa cagaagactg attgcggcgc tgccgaatac gatgccgacg gttgcgcgca
      181 aggattcagc cgcgtcggcc gcttcccgca gaattcgtgg gtgagcggct ggccatactg
      241 gcaggggacg caaatggacg agcaagccat gccgatcctg ctcgcgtggc gtctcggttc
      301 gggcgtctat aacgcgttgt ggccgaagat cgcgctcacc gctgattaca ttctccgcac
      361 agggccgtgg acctatcagg atcgctggga agaggactcg ggctattcgc catcgacgat
      421 agccgcagag gtggcgggcc tcgttgccgc tgcgcgcatt gcgcaagata acggcgacag
      481 tgcgcgcgcg ggcagctacc ttgcagccgc cgactatgat gccgtgctcg cgatgaacgt
      541 gaaggactcg gaccaggcgt ggttccgcta taacttcgac ggttacggcg aacacaatga
      601 cggcagcaat tttgacggca ccggcgttgc ccgcgcgtgg cccatcttta cagccgagcg
      661 cgggatgttc agcatcgcga gcagcggagt gggcagcagc ggcacggcgt acctcaacgc
      721 ggtcaagtcc tatgcgacgc cggaaggctt catctccgaa cagatctgga ccaacaccgt
      781 gacgctgccc ggcaactggc aggtcgtgac gccgccggaa ttcaccgccg gtgcaccgac
      841 caaatccatg gcgccgctca actgggcgat gggcgagtac atcagcctgc tggcatcgat
      901 tcacgcgggc cgggtcgtcg atattccgtc catcgtgtgt gcgcgttata gcaattgcgt
      961 gatcgcggcg gcaagcgggc aggccggcgt cacgatcagc gtcaaggccg cgacgcagcc
     1021 ggggcagtac atgtatgtaa ccggcagcac ggacgcgctc ggcaactgga acaccgacct
     1081 cggactgccg gtggacccgg ccaattatcc ggtgtggagc aacgcggtga atctgccggc
     1141 gagcagcagc gttcaataca agtactaccg caagaacgcc gatggaagcg tgacatggga
     1201 gaaggtatcc ggaggtggaa atcgcacgct gaacgtgccg gcgtcgggcg gcgtcacatt
     1261 aaacgatacg gtcggctggt aagcgtcgag cggtgtttga gaccacgcag taagcaaacc
     1321 tctcccggtc gcgccggatc gggcagacat ttctgtatca cttttaaacc agccggcaaa
     1381 tgcttatgcg accggggttt tgccgcccgc aaaacagcgg ttgcaaatgg cgtttcaacg
     1441 atcggatgcg cctccagcaa ggccttgagt acacgaaagc gtttgcgcga ttagtcggat
     1501 ggatggcact cgttcgatcg aacgctgttc aagagtgaat caaaaacgtt ctcgtcccca
     1561 ctgtgcgctg ttgcatatcc agggaagcgc catgctggca tagggttttc gagaactggc
     1621 acggtcctcg ctatatgtgt ggtgcagcac tagccagggt cgagattcga aatcgagcaa
     1681 acgacgctgg cagatctgct tacggggctg gtcgcaacat gtcttcgatg gatgccgtca
     1741 gatgcatcac gagacgtgag gtggaccaga ataaaaaata cgcgcacagc gcacgaatca
     1801 gagaagaaaa aatcatgttg accctcaaat cagacctgca cggcaatcat ctgttgggcg
     1861 cgcttccggc gcacgaatgg caagcgctta cgccgcacct ggaactcgtt cagcttcgta
     1921 caggccaatt gctttgcgat tcagggcaac ggattcatca cgtgtatttc ccgacgacgg
     1981 ccatcatctc tttgcttcac tcgatggaag acggcagctc ggcggagatc gcggccatcg
     2041 gtcgtgaagg catgaccggc gttcctatcc tgaccggcgg cgacaccatg ccgacgcgcg
     2101 ttcaggttca atgcccgggc tttgcgtatc gcatgagcgc tgcggccttg cgtgagcagt
     2161 tcaatcgctc ggatttcctg cgccgcctga tgctgctgta catgcaagcg ctgctgacgc
     2221 aagttgcgca gacggccgcg tgcaaccgtc accactcgct gagcaagcag ttgtgccgct
     2281 ggttgctgat tgagattgac cgtacgccgt ccaacgaatt ccaggtgacg cagcagatga
     2341 ttgccgacat gctgggcgtg cg
//
LOCUS       DATASH010000933         8106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4906377,
            whole genome shotgun sequence.
ACCESSION   DATASH010000933 DATASH010000000
VERSION     DATASH010000933.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 8106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8106
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4906377"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2654)
                     /locus_tag="VHV99_24950"
     CDS             complement(<1..2654)
                     /locus_tag="VHV99_24950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011353392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEX3638670.1"
                     /translation="MTARLPIDGTPALADYKLSDNLTATRGRIFLTGTQALVRLALMQ
                     RAADRARGMNTAGFISGYRGSPLGMVDQQLWKAKKLLTASDIRFLPAINEELGGTAVL
                     GTQRVESDPERTVEGVFAMWYGKGPGVDRAGDALKHGNAYGSSPHGGVLVVAGDDHGC
                     VSSSMPHQSDFALMAWHMPVVNPSNIADMLEFGLYGWALSRSSGAWVGYKAISETVES
                     GSTVDLDALQTEWATPQDFETPAGGLHNRWPDLPSLTIESRMHAKLDAVRHFARTNSI
                     DKWIAPSPHANVGIVTCGKAHLDLMETLRRLDLTIADLETAGVRIYKVGLSFPLEMTR
                     IDAFVSGLSEVLVIEEKGPVIEQQIKDYLYNRTQGTRPIVVGKNAEDGTLLLSSLGEL
                     RPSRILPVFANWLAKHKPALDRRERVVDLVAPQILSNAADSVKRTPYFCSGCPHNTST
                     KVPEGSIAHAGIGCHFMASWMERDTTGLIQMGGEGVDWASHSMFTKTRHVFQNLGDGT
                     YFHSGILAIRQAVAAKATITYKILYNDAVAMTGGQPVDGSISVPQIARQVEAEGVSRF
                     VVVSDEPEKYDGHHDQFPKGTTFHHRSEMDTVQRELRDTDGVTVLIYDQTCAAEKRRR
                     RKKGEFPDPDKRLFINEEVCEGCGDCGVQSNCLSVEPVETALGRKRRIDQSSCNKDYS
                     CVNGFCPSFVTVEGGKLKKAAGAAFDPVALAARVEALPIPATHLDAAPYDILVTGVGG
                     TGVVTVGALISMAAHLEGKSASVLDFMGFAQKGGSVLSFVRFAARDEWLNQVRIDTQQ
                     ADVVLACDMVVGASADALQTVRHGRTRIVANTHPIANATFVQNPDASLHADALVDKMR
                     HAAGAEFMSTCDAQALSAKFLGDTIGAN"
     gene            complement(2947..3942)
                     /locus_tag="VHV99_24955"
     CDS             complement(2947..3942)
                     /locus_tag="VHV99_24955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HEX3638671.1"
                     /translation="MATEPSDRFSGIGDDEARAQKRRARGSRELRLDDRVVIAHGMPT
                     PLWQDLYHRALVVRWPTFFVSLGVLFVLLNTVFASLYMFGRAPIANQFPAGFGGAFFF
                     SVETLATVGYGDMHPQTVYAHWIATLEIFVGMSGIALATGLIFARFSRPHAKIMFARY
                     AVVRPLEGRMTLMVRAANARQNVIAEARAKLRMMRLDTTVEGYTLRKLYDLTLVRDQH
                     PVFKLGWVMMHVIDENSPLFGETAETLKGRDASLLLTLEGVDESTSQTMQARHMWPCE
                     QILWQYRFVDVMREEGGVSHIDYSHFDEVVPLDSAPAASVAQAPAAAEPATGSRS"
     gene            complement(4179..4424)
                     /locus_tag="VHV99_24960"
     CDS             complement(4179..4424)
                     /locus_tag="VHV99_24960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013337813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638672.1"
                     /translation="MAEQLFLYGVYSIHVRPLELNGARWDAEYEIRHREKAVKPWTTI
                     GGDSGYGDEAEAVAAAHQQAVDDIEHGAGIPKPRAFP"
     gene            complement(4486..4710)
                     /locus_tag="VHV99_24965"
     CDS             complement(4486..4710)
                     /locus_tag="VHV99_24965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638673.1"
                     /translation="MDDITYTKGIYTATASTREVKSDTWQGVVTLSRDEGDADQENTV
                     YEVDATSSTPEEALEEAKALAHRILGEIEL"
     gene            complement(4838..5755)
                     /locus_tag="VHV99_24970"
     CDS             complement(4838..5755)
                     /locus_tag="VHV99_24970"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HEX3638674.1"
                     /translation="MSNLSATTEFPFFVSAGDILTDLVRTGASQWLSRPGGAGWNVAR
                     CVARLGLPTACAGSLGVDNFSDDLWNASVAAGLDMRFMQRVERPPLLAIVHQTHPPAY
                     FFMGEHGADLAFDPARLPAGWIEQVKWAHFGCISLVRQPLGDTLAGLAAELRARGVKI
                     SFDPNYRNLMEQGYEPTLRKMAALADLIKVSDEDLRMLFKTDDEAGALAQLRAMNPAA
                     TVLVTRGPETSMLIDGAMMVEARPPRVEVVDTVGAGDASIGGLLFSLMTAPQRTWPEH
                     LAFSLAAGAAACRHAGAHAPTLDEVVALL"
     gene            complement(5761..7032)
                     /locus_tag="VHV99_24975"
     CDS             complement(5761..7032)
                     /locus_tag="VHV99_24975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HEX3638675.1"
                     /translation="MTQSDPLHPANGVAVPAVDSFRSRDFLLSHVQDTLRFYAPHVFD
                     PSGGFFHFFRDDGSVYDKTTRHLVSTCRYVFNYAMAYRQFGDPQHLEYARHGLRFLRE
                     AHWDAGHEGYDWELEWHDGHKRTLDATRHCYGLAFVLLAYSHAAMAGIEEARPMIGAT
                     FELMEHRFWDAAAGLYADEATPDWRVSSYRGQNANMHTTEALLAAHEATGHLVYLDRA
                     ERVASNITLRQAKLSQGLVWEHFHADWSVDWHYNEEDSSNIFRPWGFQPGHQTEWAKL
                     LLILERFRPLPWLLPRAIELFDAAMTHAWDEDHGGLYYGFGPDGTVCDHDKYFWVQAE
                     TFATAALLARRTGNERFWDWYDEIWRYSWAHFVDHKYGAWYRILTCDNRKYSDEKSPA
                     GKTDYHTMGACYEVLAHALPGSAATASESAE"
     gene            complement(7029..8015)
                     /locus_tag="VHV99_24980"
     CDS             complement(7029..8015)
                     /locus_tag="VHV99_24980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX3638676.1"
                     /translation="MATTIRDVARAASVSIGTVSRALKNQPGLSEATRERVIEAARQL
                     GYDAAQLRPRIRRLTFLLHRQHNNFAVSPFFSHVLHGVEDACRERGIVPSVLTAGPTE
                     DVIQQMRLHAPDAVAIAGFVEPETLSTLVAMQRPLVLIDLWAPGLRSVNLDNAAGATL
                     AMRHLFEQQRKRVAFIGGSLAHFSIAQRALGYRRAFFEAGLLFDPSLEVTIDAGLDPD
                     SGAARAMHRLLDAPGPRPDAVFAYNDAAALAALRACLARGIRVPEDIAIIGFDDIPAA
                     AHATPPLSTISVDKEALGRRGVELLLEDMPAELEVHLPVHLIARASTLVKTP"
BASE COUNT         1461 a         2531 c         2630 g         1484 t
ORIGIN      
        1 atgttcgcgc cgatcgtgtc gcccaaaaac ttcgctgaga gcgcctgtgc gtcgcaagtc
       61 gacatgaatt ccgcgcccgc cgcatggcgc atcttgtcca ccagtgcgtc cgcgtgcagg
      121 ctcgcatcgg ggttttgtac gaacgtcgcg ttcgcaatcg ggtgcgtgtt ggcgacgatg
      181 cgcgtgcggc catgacgcac ggtctgaagc gcgtcggcgc tcgctccgac gaccatgtcg
      241 cacgcaagca ccacgtcggc ctgctgcgta tcgatacgca cctggttgag ccactcgtcg
      301 cgcgcggcga atcgtacgaa cgaaagcacg gagccgccct tttgcgcgaa gcccatgaag
      361 tcgagcaccg aggcgctctt gccttccaga tgcgcagcca tgctgatcaa tgcgccgacg
      421 gtcacgacac cggtgccacc cacgcccgtc accaggatgt catacggcgc ggcatcgaga
      481 tgcgtcgcgg gaatcggcaa cgcttcgacg cgtgcagcga gggccacagg atcgaacgcg
      541 gcgcccgcgg ctttcttcag cttgccgcct tccacggtga cgaagctcgg acagaatccg
      601 ttcacgcagg aatagtcttt attgcacgac gattgatcga tgcggcgctt gcgccccaac
      661 gcggtttcaa ccggttcaac cgacaaacag ttcgactgca cgccgcaatc cccacagcct
      721 tcgcaaactt cctcgttgat gaagagacgc ttgtccggat cgggaaactc gccttttttc
      781 ctgcgacgcc gtttttccgc cgcacaggtc tggtcataga tcagcacggt cacgccgtcg
      841 gtatcgcgca attcgcgctg gacggtgtcc atttcgctgc gatggtggaa cgtcgtgccc
      901 ttcggaaact ggtcatgatg gccgtcgtat ttctccggct catcgctgac cacgacgaag
      961 cgcgacacgc cttcggcctc cacctgacgc gcgatttgcg gcacggagat actgccgtcg
     1021 accggttggc cgccggtcat cgcgaccgcg tcgttataga gaatcttgta cgtgatcgtc
     1081 gctttcgcgg ccaccgcctg gcggatcgca agaatgcccg agtgaaagta ggtaccgtcg
     1141 ccgagattct ggaacacgtg gcgcgttttc gtgaacatcg aatgcgaggc ccaatcgacg
     1201 ccttcaccgc ccatctgaat caggccggtg gtatcgcgct ccatccacga cgccatgaaa
     1261 tggcagccga tccccgcatg cgcgatcgag ccttcaggca ctttggtcga cgtgttgtgc
     1321 gggcagcccg agcagaaata cggcgtacgt ttcacgctat ccgcggcatt cgagaggatc
     1381 tgcggcgcga ccagatcgac cacacgctcg cggcgatcca acgccggctt gtgtttcgcg
     1441 agccagttcg cgaacaccgg cagaatgcgt gacggccgca attcacccag cgacgacagc
     1501 aaaagcgtgc cgtcttcggc attcttgccc accacgatag gacgcgtgcc ttgcgtgcgg
     1561 ttgtacagat agtctttgat ctgctgctcg atgaccgggc ctttttcctc gatcaccagc
     1621 acttcggaca aaccggacac gaacgcgtcg atgcgcgtca tttccaacgg gaacgacagc
     1681 cccactttgt agatccgcac gccggccgtt tccagatcgg cgatggtcag atcgagccgg
     1741 cgcagcgttt ccatcaagtc gagatgcgcc ttgccgcacg tcacgatacc cacgttcgca
     1801 tgcggactcg gcgcaatcca tttgtcgatg ctattggtgc gcgcgaaatg gcggacggcg
     1861 tcgagctttg cgtgcatccg cgattcgatc gtcaggctgg gcagatcggg ccagcggtta
     1921 tgcaggccgc ctgcgggtgt ttcgaaatcc tgtggggtcg cccattcggt ttgcagcgcg
     1981 tcgaggtcta cggtcgagcc ggattcgacg gtttcggaaa tcgccttgta gccgacccac
     2041 gcacccgagg agcgcgacag cgcccagcca tacaggccga actcgagcat gtcggcaatg
     2101 tttgacggat tcaccaccgg catatgccac gccatcaggg cgaaatcgct ctggtgcggc
     2161 atcgacgacg acacgcagcc gtggtcgtcg cctgccacca ccagcacgcc gccgtgcggc
     2221 gacgagccgt acgcattgcc gtgtttcagc gcatcgcccg cgcgatccac gccggggccc
     2281 ttcccgtacc acatcgcgaa cacgccttcg acggtgcgct cagggtccga ttcgacccgc
     2341 tgcgtaccga gcaccgcggt gccgcccagt tcttcgttga tcgccggcaa aaagcgaata
     2401 tcactcgccg tgagcagttt tttcgctttc cacaactgct ggtcgaccat gccgagcggc
     2461 gagccgcgat agccgctgat gaagccggcc gtgttcatgc cgcgcgcgcg gtcggctgcg
     2521 cgctgcatca gcgcgaggcg caccagcgct tgcgtgccgg tcaggaagat gcggccgcgc
     2581 gtggcggtga ggttgtcgga cagcttgtag tcggcgagag cgggcgtgcc gtcgatgggc
     2641 aggcgagcgg tcatgggtga gtctccattt gtcgatcgga gtcaatgctt cagcactgac
     2701 tccgatccca tgcaatttga ttgctgtttg catccgcgcg ccgtggaggg ggaccacaaa
     2761 aggtgacgcg gcgaaagaga ctctattttt tagcgccgat tggagtgaat tttttctact
     2821 tggcgggacg ttcaacgaaa cagcgcgacg agccacgcgt tttaatgaag ttttgagatg
     2881 gatctgccac gcggcggccg ggttcgggta tgccctggtg tatttccgca cgtacggcgc
     2941 gcgactttaa ctccgtgaac ccgtggctgg ttctgccgct gcgggagcct gcgccacact
     3001 cgcggccggc gcagagtcga gcggcaccac ctcgtcgaaa tgcgagtaat cgatgtgact
     3061 cacaccgcct tcctcgcgca tgacgtcgac gaagcggtat tgccagagga tctgctcgca
     3121 aggccacatg tggcgtgctt gcatggtctg cgaggtcgat tcgtcgaccc cttcgagcgt
     3181 gagcagcagc gacgcgtcgc ggcccttgag cgtttcggcg gtttcgccga acagcgggct
     3241 gttctcgtcg atgacatgca tcatgaccca gcccagtttg aacacgggat gctggtcgcg
     3301 taccagcgtc agatcgtaga gcttgcgaag cgtataacct tcgacggtgg tgtccagccg
     3361 catcatgcgc agcttcgcac gcgcttcggc gatgacgttc tggcgcgcgt tggcagcgcg
     3421 caccatcagc gtcatccggc cttcgagcgg ccgtacgact gcatagcggg cgaacatgat
     3481 cttggcgtgc gggcgtgaga agcgcgcgaa gatcagcccc gtcgccaacg cgatgccgga
     3541 catgccgaca aagatttcga gcgtcgcgat ccagtgcgcg tagacggttt gcgggtgcat
     3601 gtcgccatag ccgaccgtcg cgagcgtttc gacgctgaag aaaaatgcgc cgccaaagcc
     3661 cgctggaaac tgattggcga tcggcgcccg gccgaacatg tagagcgagg caaacacggt
     3721 gttgagcagc acgaacaaaa ccccgagcga gacaaagaac gtcggccagc ggacgacgag
     3781 tgcacggtgg tagagatcct gccagagcgg cgtcggcatg ccgtgcgcga tgactacacg
     3841 atcgtccagg cgcaattccc ggctgccgcg cgcgcgacgt ttctgcgcac gcgcttcgtc
     3901 gtcgccgatg ccggagaagc ggtctgacgg ttcagtagcc atcgcgtgcc cgtggagaag
     3961 aaaacgcgca aagcgtagca cgccggttag cgcggggaag gtgattttcg gcgcgctgca
     4021 ggaacggtgg aaggagcgcc tctacggcgt gctgcagggg cgtcggcggt gcttccaagg
     4081 cgtgcttgcg gtgcttgggt gcttcgtaca agcgcacggc gaacctgcga actcgctcgc
     4141 gtgacgcaca cgccaccatc aatgcgctcg ccgtgggtct acggaaaggc tcgcggcttc
     4201 ggaatgccgg cgccgtgttc gatgtcgtcg acggcttgct ggtgcgcggc ggcgacggct
     4261 tccgcctcgt cgccgtagcc gctatcgcca ccgatggtgg tccacggctt gacggccttc
     4321 tcgcgatgcc ggatttcgta ttccgcgtcc cagcgtgcgc cgttgagttc gagagggcgg
     4381 acgtgaatcg agtacacgcc gtagaggaag agctgttcag ccatgatcgc ctccagccgg
     4441 ttgattcaga ggtggcgcac tgcgacgggc gcggcgccgg gttgcctaga gttcgatttc
     4501 cccgaggatg cggtgcgcga gggccttggc ttcctcgagc gcttcttccg gcgtggacga
     4561 ggtggcgtcg acttcgtaga ccgtattctc ctggtcggcg tcgccttcgt cgcgggaaag
     4621 ggtgaccaca ccctgccacg tgtcgctctt tacttctcgt gttgatgcgg tggccgtgta
     4681 gatgcctttg gtataagtga tgtcatccat ggcgacgctc cttgttccaa aggtgtgggt
     4741 tttttcatcc tagcaccgat ggtcgaggtt cggttttttg cccgaacccg cttgaccacc
     4801 gcgttcacaa ccctacgcgc cgcacaagac aaacaagcta cagcagcgcc accacctcat
     4861 ccagggtagg cgcatgcgca cccgcgtggc ggcaagccgc ggcgccggcg gccaaagaaa
     4921 aggcaaggtg ctccggccac gtccgctgtg gcgcggtcat caagctgaac agcagaccgc
     4981 cgatagaagc atccccggca cccacggtat cgacaacttc aacccgaggc ggccgagcct
     5041 ccaccatcat cgcgccgtcg atcagcatgg acgtctccgg cccgcgcgtc accagcaccg
     5101 tggcggccgg attcatggcc cgcaactggg caagtgcgcc ggcttcgtcg tccgtcttga
     5161 acagcatccg caaatcctca tccgacacct tgatcagatc ggccagcgcg gccatcttcc
     5221 gcaacgtcgg ctcgtaaccc tgttccatca ggttccggta attcggatcg aagctgatct
     5281 tcacgccacg tgcccgcaac tcggcggcca acccggcaag cgtgtcgccg agcggctgac
     5341 gcaccaggct gatgcaacca aaatgcgccc acttcacctg ttcgatccag ccagccggca
     5401 agcgcgcggg atcgaaggcc agatcggcgc cgtgctcgcc catgaaaaag taggccggcg
     5461 gatgggtctg atgaacgatc gccagaaggg gcgggcgctc cacgcgctgc atgaagcgca
     5521 tgtcgagccc cgcggcgacg cttgcgttcc acaggtcgtc ggagaaatta tcgacgccca
     5581 gcgagccggc gcacgcggtc ggcaaaccga gccgcgccac gcagcgcgca acgttccagc
     5641 cggcgccgcc aggacgcgag agccattgcg aggcgccggt ccgcaccaga tcggtgagga
     5701 tgtcgccggc cgagacgaag aaggggaatt cggtggtcgc gctcaggttg ctcattttgc
     5761 ttactccgcg gattcggacg cagtggccgc gctgccaggc agtgcgtgcg ccagcacctc
     5821 atagcacgcg cccatcgtgt gatagtcggt cttgccggca ggactctttt cgtcgctgta
     5881 cttacggttg tcgcacgtga ggatgcggta ccacgcgccg tacttatgat cgacgaaatg
     5941 cgcccagctg tagcgccaga tctcgtcgta ccagtcccag aagcgttcgt tgccggtgcg
     6001 cctggcgagc agcgccgcgg tcgcgaaggt ctccgcctgg acccagaaat acttgtcgtg
     6061 gtcgcacacg gtgccgtccg ggccgaagcc gtagtagagg ccgccgtgat cttcgtccca
     6121 ggcatgcgtc atcgcggcgt cgaatagttc gatcgcacgc ggcagcagcc acggcaatgg
     6181 acgaaagcgc tcgagaatca gcagcagctt ggcccattcg gtctggtgcc ccggctggaa
     6241 tccccacggg cggaagatgt tcgaactgtc ctcctcgttg taatgccagt cgaccgacca
     6301 gtccgcgtga aagtgctccc acacgaggcc ctgcgagagt ttcgcctgac gcagcgtgat
     6361 gttcgacgcc acccgttccg cgcgatcgag atagaccagg tggccggtcg cctcgtgcgc
     6421 ggccagcagc gcctcggtgg tgtgcatgtt cgcgttctgg ccacggtacg aactgacgcg
     6481 ccagtcgggc gtcgcttcgt ccgcgtagag gccggcggcg gcgtcccaga agcggtgttc
     6541 catcaactcg aaggtcgcgc cgatcatcgg cctggcttcc tcgatgccgg ccatcgccgc
     6601 atgcgaatac gcgagcagca cgaacgcgag gccgtagcag tggcgcgttg cgtcgagcgt
     6661 gcgtttgtgg ccgtcgtgcc attcgagttc ccagtcgtag ccttcgtgcc cggcatccca
     6721 gtgcgcttcg cgcaggaagc gcaatccgtg gcgcgcgtac tcgagatgct gcgggtcgcc
     6781 gaactggcga tacgccatcg cgtagttaaa gacgtagcgg cacgtgctta ccagatgccg
     6841 cgtggtcttg tcgtagacgg agccgtcgtc gcggaagaaa tggaagaagc cgccgctcgg
     6901 atcgaacacg tgcggcgcgt aaaagcgcag cgtgtcctgt acatgcgaga gcagaaagtc
     6961 gcggctgcgg aagctgtcga cggccggcac ggcgacgccg ttggcggggt gaagcggatc
     7021 ggattgcgtc atggcgtttt caccaaagta ctggcacggg caatgagatg gacgggcaag
     7081 tgcacttcga gttcggcggg catgtcttca agcagcagct cgacgccccg ccggccgagc
     7141 gcttccttgt cgaccgaaat ggtggacagc ggcggcgtgg catgagcggc ggcgggaatg
     7201 tcgtcgaagc cgatgatggc gatgtcctcg ggcacgcgga tgccgcgcgc gaggcacgcg
     7261 cgcagcgcgg cgagcgcggc ggcgtcgttg taggcgaata cggcgtcggg gcgtgggccg
     7321 ggcgcgtcga gcaagcggtg cattgcgcgt gcggcgccac tgtcagggtc gagcccggcg
     7381 tcgatggtga cttccagcga cgggtcgaac aataagcctg cttcgaaaaa cgcgcgccgg
     7441 taaccgagcg cgcgctgggc gatgctgaag tgcgcgagcg agccgccaat gaacgcgacg
     7501 cgcttgcgct gctgttcgaa cagatgtcgc atggcgagcg tggcgccggc ggcgttgtcg
     7561 agattcaccg agcgcaggcc gggcgcccac aggtcgatca agacgagcgg gcgttgcatc
     7621 gccaccagcg tgctcagcgt ttcaggttcg acaaaaccgg cgatcgccac cgcatccgga
     7681 gcatgcaacc gcatctgctg gatcacatct tctgtcgggc cggcggtcag caccgaaggg
     7741 acgatgcccc gttcacggca cgcgtcttcc acgccgtgca gtacgtgcga gaagaacggg
     7801 ctgacggcga agttgttgtg ctgacgatgc agcagaaagg tgaggcggcg gatgcgcggg
     7861 cgcagttggg cggcgtcata gccaagctgc cgcgccgctt cgatgacgcg ttcgcgggtg
     7921 gcctcggaaa ggccaggctg gttcttcaat gcgcgtgaga cggtgccgat cgacacgctg
     7981 gccgcgcggg cgacgtcgcg gatggttgtg gccatcgaat gaggcggccg caagcggggc
     8041 ggccgcacgg tgtttgggtt cgagcgattg tatagtaaaa cgtggctaaa acagcgctgt
     8101 aaacac
//
LOCUS       DATASH010000934         3636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_951796,
            whole genome shotgun sequence.
ACCESSION   DATASH010000934 DATASH010000000
VERSION     DATASH010000934.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951796"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /gene="pdxJ"
                     /locus_tag="VHV99_24985"
     CDS             complement(<1..660)
                     /gene="pdxJ"
                     /locus_tag="VHV99_24985"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006762396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEX3638677.1"
                     /translation="MSFFLTSPNVIDLGVNIDHVATLRNARGTSYPDPIHAALMAEEA
                     GADAITLHLREDRRHIVDADVRKLRPLLKTRMNLECAVTPEMLDIACEVQPHDVCLVP
                     EKREELTTEGGLDVAGQFEAVRAACKQLADANSRVSLFIDPDETQIRAAHEAGAPVIE
                     LHTGRYAEAHDPAEQQREYERIVRAVEFGATLGLKVNAGHGLHYTNVQQIAAIEGIVE
                     LN"
     gene            complement(657..1577)
                     /gene="recO"
                     /locus_tag="VHV99_24990"
     CDS             complement(657..1577)
                     /gene="recO"
                     /locus_tag="VHV99_24990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX3638678.1"
                     /translation="MGTNDAWMTLNSDADPDDSAPAAPAREPAKPARSTRKSSSSARS
                     AQAAPGAEGEPRKAPARRAPRTPASDYRIAEQPAFVLHSYPYRETSLIIDVLSRDHGR
                     LALVAKGAKRPHSALRGVLQTFQPLALSWSGKSEVRTLTGAEWVGGMLPLAGDALLCG
                     FYVNELLVKFCAREDPHPQLFHHYVVTMTRLAHDEPPVQVLRSFERVLLRETGYAMAL
                     DRTVARKAVQAEGRYVFDPERGVREVSDDLPAQWPVIAGQTLLDMEQDDYHRAQTVAQ
                     SKTLMRFLLNTYLGGTPLATRQILIDLQNL"
     gene            complement(1564..2463)
                     /gene="era"
                     /locus_tag="VHV99_24995"
     CDS             complement(1564..2463)
                     /gene="era"
                     /locus_tag="VHV99_24995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009913163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEX3638679.1"
                     /translation="MNAPTPTGFRCGMVAIVGRPNVGKSTLMNALVGQKVSITSRKAQ
                     TTRHRITGIHTLEDAQYIFVDTPGFQTKHSGALNRSLNRAVTSTLTSVDAILFVIEAG
                     RFGPDDQKVLDLIPPSVPTLLIANKLDRVSDKDSLFPFMQQVSALHKFAEIVPLSAKN
                     PDDIKRLMATVKPFLPEGAPIYGEDDLTDRSERFLAAEILREKVFRWTGDELPYTSTV
                     LIDKFETEGRLRRIFATIMVERDTHKAMIIGQKGAKLKQISTEARLDMEKLFDGPVYL
                     ETFIKVKSGWADNEAGLRAYGYE"
     gene            complement(2519..>3636)
                     /locus_tag="VHV99_25000"
     CDS             complement(2519..>3636)
                     /locus_tag="VHV99_25000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative dsRNA-binding protein"
                     /protein_id="HEX3638680.1"
                     /translation="RFGKLDEGDLSRVRANLVKQQSLYEIAQALNISEGLRLGEGELR
                     SGGFRRPSILADTLEAVLGAIFLDGGFDAAQTVIKRLYVPILDHIDPRTLGKDAKTLL
                     QEYLQGHKIALPTYTVVATHGAAHNQQFEVECTVPKLDVKVSGSGASRRAAEQAAAKK
                     ALDEVMAAAPAVVAKPKRSKGARAAKNAEQEVVPGVTGVQAALDLRSPDRKNERGAGR
                     GESRTAPVTESAAEKSTLTATSAPLAVIRAAHVEYSAQDKSDRADKAERADKTTVHAS
                     DRAGDKATADRPAEKADAKSADGKSEGTKSMDTRAAEAKPAEAKPEAAAIRSTDKHRS
                     REATPGAAVPTPGVPAPVEHEPGVADAVQTRVADAGH"
BASE COUNT          581 a         1131 c         1193 g          731 t
ORIGIN      
        1 attcagctcg acgatccctt cgatcgcagc aatctgctgg acgttggtgt agtgcaggcc
       61 gtggcccgca ttcaccttga ggccgagcgt cgcgccgaat tcgaccgcac gcacgatgcg
      121 ttcgtattcg cgctgctgct ccgcgggatc gtgggcttcc gcgtaacggc cggtgtgcag
      181 ctcgatcacc ggcgcgcccg cttcgtgcgc ggcgcgaatt tgcgtttcgt cggggtcgat
      241 gaacagcgac acgcgcgagt tcgcgtccgc cagttgcttg catgcggcgc gcaccgcctc
      301 gaactgtccc gcgacgtcga ggccgccttc ggtcgtcagt tcctcgcgct tttccggcac
      361 gagacacacg tcgtgcggtt gcacttcgca cgcgatgtcg agcatttccg gcgtgaccgc
      421 gcattcgaga ttcatgcgcg ttttcaggag cgggcgcagc ttgcgcacgt cggcgtcgac
      481 gatatggcgg cggtcttcgc gcagatgcag cgtgatcgca tcggcaccgg cttcctcagc
      541 catcagcgcg gcgtggatcg gatcgggata agacgtgccg cgcgcgttgc gcagcgtggc
      601 gacgtgatcg atgttcacgc ccaggtcaat cacattcggc gacgtaagaa agaagctcat
      661 aagttctgca agtcgatcag gatctggcgc gtcgcgagcg gcgtgccgcc aaggtaagtg
      721 ttgagcagga agcgcatcag cgttttgctt tgcgccacgg tctgcgctcg atggtaatcg
      781 tcctgttcca tatcgagcaa ggtctgtccg gcgatcaccg gccattgtgc gggcagatcg
      841 tcggacactt cgcgcacgcc gcgctccggg tcgaacacat agcggccttc ggcctgcacg
      901 gctttgcgcg cgacggtgcg atcgagcgcc atcgcataac cggtttcgcg cagcagcaca
      961 cgttcgaacg aacgcagcac ctgcacgggc ggttcgtcat gcgcgaggcg cgtcatggta
     1021 acgacgtagt gatggaacag ttgcggatgc ggatcttcgc gcgcgcagaa tttgaccagc
     1081 agttcgttga catagaagcc gcacagcagc gcatcgcccg ccaagggcaa catgccgccg
     1141 acccattcgg caccggtcag cgtgcgcact tcggatttgc cggaccatga caacgcaagc
     1201 ggctggaagg tctgcagcac gccgcgtaaa gcggagtgcg gacgtttcgc gcccttcgcg
     1261 acgagcgcga ggcggccgtg atcgcgcgag agcacgtcga tgatcagact ggtctcgcgg
     1321 tacggatagc tgtgcaggac aaaagcgggt tgctccgcga tccggtagtc ggaagcgggg
     1381 gtgcgaggcg cgcggcgcgc cggcgctttg cgcggttcgc cttcggcgcc tggagcagcc
     1441 tgagcgcttc tggcgctcga tgaggatttc cgggtgctgc gagcgggttt ggccggctcg
     1501 cgagccggcg ctgccggcgc cgagtcgtcc gggtcagcgt cggaattcag cgtcatccac
     1561 gcgtcattcg tacccatacg cacggagtcc ggcttcgttg tccgcccagc cgctcttcac
     1621 cttgatgaag gtttccagat acaccggacc atcgaacagt ttttccatgt cgaggcgcgc
     1681 ttcagtgctg atctgcttca gcttggcgcc cttctggcca atgatcattg ctttgtgcgt
     1741 atcgcgctcg accatgatgg tcgcgaaaat gcggcgcaga cggccttcgg tttcgaactt
     1801 gtcgatcagt accgtgctcg tgtacggcag ttcgtcgccg gtccagcgaa acactttctc
     1861 gcgcaggatt tcggcggcga ggaagcgttc gctgcgatcg gtcaggtcgt cttcgccata
     1921 gattggcgcg ccttccggca ggaacggctt gacggtcgcc atcagacgct tgatgtcgtc
     1981 cggattcttc gccgacaacg gcacgatctc ggcgaacttg tgcagcgcgc tgacctgctg
     2041 catgaacggg aacagcgagt ccttatccga cacgcgatcg agcttgttcg cgatcagcag
     2101 cgtgggcacc gatggcggga tcagatcgag caccttctga tcgtccgggc caaagcggcc
     2161 ggcttcgatc acgaacagga tcgcgtcgac cgaagtcagc gtggacgtga ccgcgcggtt
     2221 gagcgagcgg ttcagcgcgc cgctgtgttt ggtctggaaa cccggcgtgt cgacgaaaat
     2281 gtactgcgcg tcttcaagcg tgtgaatgcc ggtgatgcgg tgccgcgtgg tctgcgcctt
     2341 gcgcgacgtg atactgactt tctggccgac cagcgcattc attagcgtgg acttgccgac
     2401 attcgggcgg ccgacgatcg cgaccatgcc gcagcgaaaa ccagtgggag tgggagcgtt
     2461 catattcggg ctacggcaag ttcagatcga cgcgcgggga atggcgcgcc gacggcaatc
     2521 aatggcccgc atcggcgacg cgggtttgca ccgcgtcggc tacgccgggt tcgtgttcga
     2581 caggtgccgg cacgccaggc gtgggcacgg ctgcgccggg tgtggcttca cggctgcggt
     2641 gtttgtcggt gctgcggatg gcggcggctt ccggtttggc ttcggcgggt ttggcttcgg
     2701 cggcccttgt gtccatggac tttgtgcctt cggatttgcc gtccgcggat ttggcgtcgg
     2761 ccttctcggc cggcctgtcc gccgtggctt tgtcaccagc ccgatcgctc gcgtgaacgg
     2821 tcgttttgtc tgcccgctca gccttatccg cgcggtccga cttgtcctgc gcgctgtatt
     2881 cgacgtgcgc ggcacgaatc acagccagcg gcgcggacgt tgcagtcagg gtcgacttct
     2941 cggctgcaga ctccgtcacc ggcgcggtac gggactcacc tctcccggct ccgcgctcat
     3001 tcttgcgatc cgggctgcgc aaatcgagcg cggcctgtac gccggtcacg ccggggacga
     3061 cctcctgctc ggcattcttc gccgcacggg cgcctttcga gcgcttgggc ttggcgacca
     3121 cggcgggcgc agcggccatc acctcgtcga gcgccttctt ggcggcggcc tgctcggccg
     3181 cacgacggct cgcgcccgaa ccggacacct tgacgtccag cttcggcacc gtgcattcga
     3241 cttcaaattg ctgattgtgc gccgcaccat gagtcgcgac gaccgtatac gtgggcaacg
     3301 cgatcttgtg accctgcaaa tactcctgca gcagcgtctt ggcgtcttta ccgagcgtgc
     3361 gcgggtcgat atggtcgaga atcggcacgt aaaggcgctt gatgaccgtc tgcgcagcgt
     3421 cgaacccacc gtcgaggaag atcgcgccca gcacggcttc gagcgtatcg gcaaggattg
     3481 acgggcgacg gaaaccgccg ctgcgcaact cgccttctcc cagccgcagg ccttcggaaa
     3541 tattcagggc ctgagcgatt tcgtaaagcg actgctgttt gactagattg gcacggacgc
     3601 gagacagatc gccctcgtcc agcttgccga aacgtt
//
LOCUS       DATASH010000935         6479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4513002,
            whole genome shotgun sequence.
ACCESSION   DATASH010000935 DATASH010000000
VERSION     DATASH010000935.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6479
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4513002"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1068)
                     /locus_tag="VHV99_25005"
     CDS             complement(<1..1068)
                     /locus_tag="VHV99_25005"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEX3638681.1"
                     /translation="MHIGVPAETRAHETRVAATPETVKKYVTQGHRVTIQNGAGTGAS
                     FPDEAFTAAGAEIVDGATAFGADLVLKVQSPTDAELPLLKRGATLVGMLDPFNTENSS
                     KLAAAGVTAFALEAAPRTTRAQSLDVLSSQANIAGYKAVLLAATLYPRFMPMLMTAAG
                     TVKAARVLILGAGVAGLQAIATAKRMGAVIEASDVRPAVKEQIESLGAKFLDVPYETD
                     EEREAAQGVGGYARPMPPSWLTRQSALVHERAKQADIVISTALIPGRAAPTLISVETV
                     QAMKPGSVLVDLAAGRGAEYEGRRGGNCPLTEADQVVLKHGVTIVGYTNLASMVPADA
                     SSLYARNLLDFLKLIITKEGAL"
     gene            1259..1741
                     /locus_tag="VHV99_25010"
     CDS             1259..1741
                     /locus_tag="VHV99_25010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX3638682.1"
                     /translation="MKPETWVPHVTVAAVVERNGRFLVVEEHTAEGLRLNQPAGHLEA
                     GETLLEAVVRETLEETAHPFTPEALVGIYMTHFERPGSEGVTYLRFTYCGASGEPDAA
                     RALDPDIVRTLWMSADELRACPERHRTPLVMQCLDDYLAGRRIPLDFVHTHSVGHKQT
                     "
     gene            1893..3074
                     /gene="mnmA"
                     /locus_tag="VHV99_25015"
     CDS             1893..3074
                     /gene="mnmA"
                     /locus_tag="VHV99_25015"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEX3638683.1"
                     /translation="MSKQKVVVGMSGGVDSSVTAWLLKEQGYDVVGLFMKNWEDDDDS
                     EYCSTRQDWIDVVSVADLIGIDVEAVNFAAEYKDRVFAEFLREYSAGRTPNPDVLCNA
                     EIKFKAFLDHAMSLGAETIATGHYARVRENNGRFELLKAFDHTKDQSYFLHRLNQAQL
                     SKTLFPLGEIPKTKVREIAEQIALPNAKKKDSTGICFIGERPFRDFLNRYLPTKPGPM
                     KTTEGKVVGEHIGLAFYTFGQRKGIGLGGSKDGSGEPWFVAGKDIASNTLYVAQGHDH
                     PWLLSHTLSAGNTSWVAGEPPADGFACGAKTRYRQADAPCTFGAAASAVASVAASAAG
                     AGEGLFELNFDAAQWAVTPGQSAVLYDGDVCLGGGIIEHAVTGQPTARQAQTAALLTA
                     R"
     gene            3341..4951
                     /locus_tag="VHV99_25020"
     CDS             3341..4951
                     /locus_tag="VHV99_25020"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HEX3638684.1"
                     /translation="MFSRRYLAMWCATVLLAVCAAFAATHHLSWFWIIVPAALVALGL
                     FDLTQERHAILRNYPLWGHLRFLFEFIRPEIRQYFVENDTDEKPFSRAQRSIVYQRAK
                     NDVDSRPYGTELDVKAVAHEWISHSLAPTTLDNHDFRIVVGPERKQPYSMSIFNVSAM
                     SFGSLSANAIMALNLGAKKGNFAHDTGEGSMSKYHREHGGDIIWEIASGYFGCRNDDG
                     TFSAEKFARQAAEPQVKMIEVKLSQGAKPGHGGVLPAAKITPEIAETRGVPMGRDCIS
                     PATHSEFSSPRGLLEFVDRLRTLSGGKPTGFKLCIGHPWEFFGIAKAMLETGILPDFI
                     VVDGAEGGTGAAPLEFTDHVGVPLQEGLLLVHNTLVGIGLRQRIRIGASGKMITAFDI
                     TKTLAIGADWVNAARGFMFAVGCIQAQTCHTCRCPTGVATQDPVRQRALVVPDKADRV
                     FNFHHNTLHALKEIIQAAGLKHPAELRAHHIVRRVSSHEVQLMSELLKYLDPNDLLKG
                     NYRYTLYEKYWPLAQSDSFAPKVELAAT"
     gene            5036..5311
                     /locus_tag="VHV99_25025"
     CDS             5036..5311
                     /locus_tag="VHV99_25025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638685.1"
                     /translation="MGFHIDEVRVYAFPSGAEMTDRSGPAFRPRYHHLFQSSPSLKAE
                     LVSRAVHGNIVIDSERVSGFLNDDSVRHAIAIYQVGATKIEKVWFVS"
     gene            5451..6302
                     /locus_tag="VHV99_25030"
     CDS             5451..6302
                     /locus_tag="VHV99_25030"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX3638686.1"
                     /translation="MVTPSSVPLPLGERTQLVQTRHGWMLANPNDFYLGRALIEYGEC
                     NELESAVLRQLLVRPGIVVEVGANIGVHTVPLAREAQARRQVLVAFEPQPVIFQNLCA
                     NLAANELHNVLAWPYACGAGAGTLHFTRPDYDAPGNFGGVSMQTEAGANTIAVPCVRL
                     DDVLRSHTVGLLKLDVEGFELSALQGAEETIARSRPVIYVENDRLDRSQALIEWLWSK
                     EYRMWWHLPPLFNPGNFFGRAENIYGRLLSCNMLALPRELEMEIEGFVEVTDADSATH
                     ALMSLGS"
BASE COUNT         1230 a         1973 c         2017 g         1259 t
ORIGIN      
        1 cagggcgcct tccttcgtga tgatcagctt gaggaagtcg agcaggttgc gtgcgtagag
       61 tgacgacgcg tcggcgggca ccatcgaggc cagattcgta tagccgacga tcgtcacgcc
      121 gtgtttgagg acgacctgat ccgcttcggt caacggacag ttgccgccgc gccggccttc
      181 gtactccgcg cctcggcctg cggcgaggtc caccagcacg gagccgggtt tcatcgcctg
      241 cacggtttcc accgaaatca gcgttggcgc ggcccggcct ggaatcaatg cggtggagat
      301 cacgatgtcg gcctgtttgg cccgttcatg caccaatgcc gactggcgcg taagccacga
      361 aggcggcatc ggccgcgcgt agccaccgac gccctgcgcg gcttcacgct cttcatcggt
      421 ttcgtacggc acgtcgagaa acttggcgcc aagcgattcg atctgctcct tcaccgccgg
      481 acgcacgtcg gacgcttcaa tcacggcgcc catgcgcttg gcggtcgcaa tcgcctgcaa
      541 acccgccacc cccgcgccga gaatcagcac acgggccgct ttcacggtgc cggcggcggt
      601 catcagcatc ggcataaaac gcggatagag cgtggctgcc agcagcaccg ccttgtagcc
      661 ggcgatgttc gcctgcgagc tcaaaacatc cagactctgc gcacgcgtgg tgcgtggcgc
      721 ggcctccaaa gcgaacgcgg tgacgcccgc agccgccagc tttgatgagt tttcggtatt
      781 aaaaggatcg agcatgccga ccagcgtcgc gccgcgcttg agcagcggca attcggcatc
      841 ggtcggggac tggactttaa ggacgagatc cgcgccgaat gcggtcgccc catcgacgat
      901 ttccgctccg gcggctgtaa aggcttcgtc cggaaagctc gcgccggtgc cggcgccgtt
      961 ctggatcgtg acccgatggc cctgggtcac atacttcttg accgtttcgg gcgtcgcggc
     1021 gacgcgggtt tcgtgcgcgc gcgtctctgc tggcactccg atgtgcatcg ttgttcctcc
     1081 tcgacttcct gttttacgac agatgtgccg acgaggcacg ccggcttgcg attgcaacgg
     1141 ctaacgcgtc actttaaccg aaactggggg gtgcaaagaa atcggggaca atccgggtgt
     1201 cctggccgct cgcccgcttc ccggcagccc aggcccataa acggtaaaat gcgcagccat
     1261 gaaaccggaa acctgggtgc cgcacgtcac cgttgcggcc gtcgtcgagc gtaacggacg
     1321 ctttctcgtc gtcgaggaac ataccgccga aggactgcgg ctgaatcagc ctgccggcca
     1381 tctggaagcg ggcgaaacgc ttctggaagc ggtcgtccgc gaaacgctcg aggaaaccgc
     1441 gcatccgttc acgcccgagg cgctggtcgg catctatatg acgcatttcg agcggcccgg
     1501 cagtgagggc gtgacctatc tgcgcttcac ctattgcggc gcgagcggcg aaccggacgc
     1561 ggcacgcgcg ctcgatcccg acatcgtccg cacgctgtgg atgagcgccg acgaattgcg
     1621 cgcctgcccc gaacggcatc gcacgccact cgtcatgcaa tgtctcgacg attacctcgc
     1681 gggacggcgt atcccgctcg acttcgtgca cacgcattcg gtcgggcata agcagacgta
     1741 agcggctgta ggctgtgtcg ttagggatag ccagacatag cccggcctaa cccgtcatag
     1801 acgggcctga gccgccacaa gcggccaatc aggcgaccaa accacggtca acccacgatc
     1861 aatgaagtca cgcagcaccc agtgagcagc ttatgagcaa acagaaagtc gtagtaggca
     1921 tgtcgggcgg cgtcgattcg tcggttaccg cgtggctgct gaaggaacag ggctacgacg
     1981 tggtcggcct gttcatgaaa aactgggaag acgacgacga tagcgaatac tgctcgacgc
     2041 ggcaggactg gatcgacgtc gtctcggtgg cggacctgat cggcatcgac gtcgaagcgg
     2101 tcaacttcgc cgccgaatac aaagaccgcg tgttcgccga attcctgcgc gaatactcgg
     2161 ccggccgcac gccgaacccg gacgtgctgt gcaacgccga gatcaaattc aaggccttcc
     2221 tcgaccacgc gatgtcgctc ggcgcggaaa ccatcgcgac cggccactat gcgcgtgtac
     2281 gtgagaacaa cggccgcttc gaactgctga aggccttcga tcatacgaag gatcagtcgt
     2341 acttcctgca tcggctgaac caggcgcagt tgtcgaagac gctgtttcct ctgggcgaaa
     2401 ttccgaaaac caaagttcgc gagatcgccg aacagatcgc gctgccgaat gcgaaaaaga
     2461 aagattccac cggtatctgc ttcatcggcg aacggccgtt ccgcgacttc ctgaaccgct
     2521 atctgccgac caaacccggt ccgatgaaaa ccaccgaagg caaagtggtc ggcgaacata
     2581 tcggcctcgc gttctacaca ttcggacagc gcaagggcat tggcctcggt ggcagcaagg
     2641 acggcagcgg cgagccgtgg ttcgtcgccg gcaaggatat tgcgtcgaac acgctgtatg
     2701 tcgcgcaagg gcacgaccat ccgtggctgt taagccatac gttgagcgcg ggcaatacga
     2761 gctgggttgc gggcgagccg ccggccgacg gcttcgcgtg cggcgcgaaa acgcgttacc
     2821 ggcaagcgga cgcgccgtgc acgttcggtg cggctgcaag tgcggttgca agtgtggcgg
     2881 caagcgcggc gggtgccggc gagggtctgt tcgaactgaa tttcgacgcc gcccaatggg
     2941 ctgtcacacc ggggcaatct gcggtgctct acgatggcga cgtgtgtctc ggcggcggca
     3001 tcatcgaaca cgcggtgacc gggcagccca cggcgcgcca ggcgcaaacg gccgcgttgc
     3061 taactgcccg ttgagccagg agttttatcg tgtggcaacg tttagcatcg ctcgacgtcg
     3121 ccgcacgcgc ttcactttcc cggcttatct tcagccttca aacttcaggc ttcaaacttc
     3181 aaccttcacg cacttcacgc ttcactcacg cgtttcatct tcgagccgca gccagcttca
     3241 ccctaacaag cagttgtgcg tttcccttcg cggcggattc cgtctgcgcc gacagcgtcg
     3301 acgatccctc cgtgacgatc ttcactgcac tggagtcccc atgttttctc gacgttatct
     3361 ggcgatgtgg tgcgcaaccg tgctgctcgc cgtctgtgcg gcgttcgccg cgacgcatca
     3421 cctttcgtgg ttctggatca tcgtgccggc ggcgctggtc gcgctcggcc tgttcgacct
     3481 gacgcaggaa cgccacgcga ttctgcgcaa ctatccgctg tggggccact tgcgtttcct
     3541 gttcgaattc atccgcccgg aaatccgcca gtatttcgtc gagaacgata ccgacgagaa
     3601 accgttctcg cgcgcgcaac gcagcatcgt ctatcagcgc gcgaagaacg acgtggacag
     3661 ccgcccgtac ggcaccgagc tcgacgtcaa agccgtcgcg catgaatgga tcagccactc
     3721 gctcgcgccg accacgctcg ataaccacga cttccgcatc gtcgtcgggc ccgagcgcaa
     3781 gcagccctat tcgatgtcga tcttcaacgt ctccgcgatg agcttcggct cgttgtcggc
     3841 gaacgcgatc atggcgttga acctcggcgc gaagaaaggc aacttcgcac acgacacggg
     3901 cgaaggttcg atgtcgaaat accaccgcga gcatggcggc gacatcatct gggaaatcgc
     3961 ctcgggctac ttcggctgcc gcaacgacga cggcaccttc agcgccgaga agttcgccag
     4021 gcaggccgcc gaaccgcaag tgaagatgat cgaggtgaag ctctcgcaag gcgcgaagcc
     4081 cggtcacggc ggcgtgctgc cggccgccaa gatcacgccc gagattgcgg agacgcgcgg
     4141 cgtgccgatg ggccgcgact gcatctcgcc cgccacccac tccgaattct cgtcgccgcg
     4201 cggcctgctc gaatttgtcg accgtttgcg cacgctgtcc ggcgggaagc cgaccgggtt
     4261 caagctgtgc atcgggcatc cgtgggaatt cttcggcatc gcaaaggcga tgctggagac
     4321 ggggattctg ccggacttca tcgtggtcga cggcgcggaa ggcggcacgg gcgcagcgcc
     4381 attggagttt accgatcacg tcggcgtgcc cttgcaggaa ggcctgctgc tggtgcacaa
     4441 cacgctggtc ggcatcggct tgcgtcagcg catccggatt ggcgcgagcg gcaagatgat
     4501 taccgcgttc gacatcacca agacgctggc gatcggcgcc gactgggtca acgcggcgcg
     4561 cggcttcatg ttcgcggtcg gctgcattca ggcgcagacc tgtcacacct gccgctgccc
     4621 gacgggcgtc gccacccagg acccggtgcg tcagcgtgcc ttggtggtgc cggacaaggc
     4681 cgaccgcgtg ttcaacttcc atcacaacac gttgcatgcg ctgaaggaaa tcattcaggc
     4741 cgcggggctc aagcaccctg ctgaactacg cgcgcatcac atcgtgcggc gcgtgtcgtc
     4801 gcatgaggtg cagttgatgt ccgaactgtt gaagtatctc gatccgaatg atctgctgaa
     4861 gggcaactat cgctatacgc tgtacgagaa gtattggcct ttggcgcaaa gcgattcgtt
     4921 tgcgccgaaa gttgaactgg cggcgactta acccggttat ggtggcgatc ggctggcgtg
     4981 ccgatcgtcg caacgtttta gacgtggatt acgagttcag gatctggaag cgtttttggg
     5041 tttccatatc gatgaggtga gggtctatgc gttcccgtca ggcgccgaga tgacagatcg
     5101 ttccggtccg gcctttcgcc cccggtatca tcacttgttc cagtcgagcc cttcgctcaa
     5161 ggccgagctt gtaagtcgtg ctgtccacgg caacattgtg atcgacagcg agcgtgtgtc
     5221 gggctttctg aacgacgatt cagtccgcca cgcgattgcc atctatcaag tgggtgctac
     5281 gaagatcgag aaggtttggt tcgttagcta ggaaatcagg aagtcctcgc ggagtcttag
     5341 gttttgacgg cgagttcttg cgcaaaaata agaatactta agactccgtc ttatatacaa
     5401 ggacagcggc ggaccgtaca tttgcgtggt cttgttcccg ctcaatgttc atggttacgc
     5461 cctcttctgt tccgctgccg ctcggcgagc gcacgcaact cgtccagacc cgccatggct
     5521 ggatgctcgc caatccaaac gacttctatc tcggccgcgc cctcatcgaa tacggtgaat
     5581 gcaacgaact cgaatctgct gtgttgcgac agttgctggt gcggccgggg atcgtcgtgg
     5641 aagtcggcgc gaatatcggc gtgcataccg tcccgttggc gcgtgaggcg caggcgcgcc
     5701 gccaggtgct ggtcgcgttc gagccacagc cggtgatctt tcagaacctc tgcgctaatc
     5761 tcgctgccaa cgagttgcat aacgtgctgg catggccgta tgcgtgtggg gcgggtgcag
     5821 ggacgctgca tttcacgcgg cccgactatg acgcaccggg taacttcggt ggcgtgtcga
     5881 tgcagacaga ggctggggcg aacacgatcg ccgtgccgtg cgtgcggctg gatgacgtac
     5941 tgcgctcgca taccgtcggg ctgctgaaac tcgacgttga aggcttcgag ttgagcgcgc
     6001 tgcaaggcgc ggaggaaacc atcgcgcgct cgcggccagt gatttacgtg gagaacgacc
     6061 ggctcgatcg gtcgcaggcg ttgatcgagt ggttgtggtc gaaggagtat cggatgtggt
     6121 ggcatttgcc gccgctgttc aatccgggga atttctttgg gcgcgcggag aatatctacg
     6181 gcaggttgct gtcgtgcaat atgctcgcgc tgccgcgcga gttggagatg gagattgagg
     6241 ggtttgtcga ggtgacggat gcggattcgg caactcatgc gttgatgagt ttgggtagtt
     6301 agcggagcgc gaggcgacga aatccaactt ccaggtgaaa agcacggcaa acgggcagtc
     6361 atacgtgaac ggtcatttga agatcgcacc attattgctg ccatgtggtg gccacgaatg
     6421 ctgactctct tccccacgca ctaaacgaca acgggccgct caaagcggtc cgttgccgc
//
LOCUS       DATASH010000936         3235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_751705,
            whole genome shotgun sequence.
ACCESSION   DATASH010000936 DATASH010000000
VERSION     DATASH010000936.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751705"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="VHV99_25035"
     CDS             complement(<1..905)
                     /locus_tag="VHV99_25035"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018434358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX3638687.1"
                     /translation="MAIYLDDPQRKALARQAASWTWRTQWPTWLLIVTIYGGWFGVAT
                     HACALGLPLTTVLLALLGTWYLSLQHELLHGHPTRSPLFNALLGFAPLAVWFPYGVYR
                     DSHLQHHDDPHLTRPERDPESYFVSQHVWQRAGFAIRALLIFRNTFVGRLLVGPAFAI
                     AATGADALDKIKHGDWRDVPTWLAHLTVLAALTVWLQRVCGIPAWVFIVGAGYGSLSL
                     GSIRSFQEHRAAQAHEHRTVINEAAWFWRLLFLNNNYHLVHHDLPHVPWFALRRVFEM
                     SRQQYIERSGGFLVKGYSEWLRLYA"
     gene            complement(1187..1963)
                     /locus_tag="VHV99_25040"
     CDS             complement(1187..1963)
                     /locus_tag="VHV99_25040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HEX3638688.1"
                     /translation="MTLRKTTRQQLIILRIAAALAVAFTATACARLADVDPDALWKIV
                     DLRCVPSQQATGTPGQCTTVDLDKRYVILKDIVGRSQHLLIPTDRVTGIESPFILAPN
                     AQDYWDDAWASRRYVEKSVKRSLPDNQLGLEINSQYRRSQNQLHIHIDCLHGNVSDTL
                     ARHAKDAPGEWHWDTLDGQRYRIMRVTSLTQANNPFRIVARDNRNTAAMAMQTILVTG
                     AGPSAQKDGWLIVNSGLDADNGSGSAESLLDHACRVADGG"
     gene            complement(2121..>3235)
                     /locus_tag="VHV99_25045"
     CDS             complement(2121..>3235)
                     /locus_tag="VHV99_25045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013342953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX3638689.1"
                     /translation="LLDAGAIVLGKANMHELAFGITSTNLAVHAGPVRNPYDPSLIPG
                     GSSGGTAVAIAARIVPAGLGTDTGGSARIPAALTGTAGFRPSVGNGGAERRYYDPNAV
                     VPISHTRDTVGPMARTVADIALLDGVITGAGPLPALTLSGLRIGLPAPLWEGLERQVE
                     DVARAALRRLEAAGVTFVPVAMSELETLNGQVGGPLAIHEPREDVLAWLVANDAPVRT
                     VAELATRIASPDVRAIYDGVLADVLGAHYDAALKQWRPRLQRYVAATFADERLDALLF
                     PTTRLVAVQIDDLNGSSTVSIDGAASIDTMDAFLRNTDPASTSGIPGLSLAAGMSASG
                     LPVGLELDGPLGDDRRLLAIGVAVEQLLGVLPAPVL"
BASE COUNT          608 a          999 c         1054 g          574 t
ORIGIN      
        1 aatgcataaa gcctgagcca ttcgctgtaa ccctttacca gaaagcctcc cgagcgttct
       61 atgtactgct ggcgagacat ttcaaagacc cgtcgaagcg cgaaccacgg cacatgcggc
      121 aagtcatgat gcaccagatg gtagttattg ttcaggaaca gcaatcgcca gaaccaggcc
      181 gcctcgttga tgacggtgcg atgctcgtga gcctgtgccg cccgatgttc ctgaaacgag
      241 cggatcgagc ccaacgacaa cgagccataa cccgcgccga cgataaacac ccacgcgggg
      301 attccacaga cgcgttgaag ccacaccgtc agcgcggcta gcacggtcag atgcgcgagc
      361 cacgtgggca cgtcgcgcca atcgccgtgt ttgattttgt cgagcgcgtc cgcgcccgtt
      421 gccgcaatgg caaaagcggg tccgaccagc aagcggccga cgaacgtatt gcggaagatc
      481 agcagcgcgc gaatcgcgaa acccgcacgt tgccacacat gctggctgac gaaataactt
      541 tcgggatcgc gctctggacg tgtcagatgc ggatcgtcgt gatgctgcag atgcgaatcg
      601 cgatagacgc catagggaaa ccacaccgcg agcggcgcga acccgagcag tgcgttgaag
      661 agcggtgagc gggttgggtg accgtgcagc aattcatgtt gcagggagag ataccacgtg
      721 ccgagcaacg ccagcagcac ggtggttagc ggcaggccga gcgcgcacgc atgcgtcgcc
      781 acgccgaacc atccgccgta gatcgtgacg atcagcagcc acgtcggcca ttgcgtgcgc
      841 catgtccagc ttgcagcctg gcgggcgagt gctttgcgtt gcgggtcgtc gaggtaaatc
      901 gccatcggct ggatcggtcg gaatgcaggt ttggaccgat cctacggacc aattcacgcg
      961 gttagaacca ttttgttcag caatgcatct gcaaaaacgt gcatagagca gggggacata
     1021 ttgctgtgcc ttagccggca ccctggtcgg tgtcagtcag ccgtcgccgg gcgacaggca
     1081 agccttgcaa ggcactcgtc agttctccgc aggtgagccg tcagttctcc gcagaggagc
     1141 cgtcagttct ccgcagttga gtcgtcaatt tgccgcagat gagccgtcaa ccaccgtccg
     1201 ccacccggca ggcatgatcg agcaaacttt ccgccgagcc gctgccgttg tctgcatcga
     1261 ggccgctatt cacgatgagc cagccgtcct tctgcgccga tggccccgcg cccgtcacga
     1321 ggatggtttg catcgccatt gccgcggtgt tcctgttgtc acgcgcgacg atgcgaaacg
     1381 gattgttcgc ctgagtgagc gaggtgacgc gcatgatccg gtagcgctgg ccgtcgagcg
     1441 tgtcccaatg ccactcgcct ggcgcgtcct tcgcgtgacg cgcgagcgtg tcgctcacat
     1501 tgccgtgcag gcaatcgata tggatatgca actggttttg cgagcggcgg tattgcgagt
     1561 tgatttcgag gccgagttga ttgtcgggca aggaccgctt gacggacttc tcgacatatc
     1621 gccgcgatgc ccatgcatcg tcccagtagt cctgtgcgtt cggcgccaat atgaatgggc
     1681 tttctatgcc ggtgacgcgg tcggtcggaa tcagcagatg ctgtgagcgg ccgacgatat
     1741 ccttcaggat cacgtagcgt ttgtcgagat cgacggtcgt gcattggccc ggtgtaccgg
     1801 tggcctgttg cgacggcacg cagcgcagat cgacgatttt ccagagcgcg tctggatcga
     1861 cgtcggcaag gcgcgcgcag gcggtggcgg tgaaggcgac cgccagggcg gcggcgatgc
     1921 gcagaatgat tagctgttgg cgggtggttt ttcgcagtgt catcggcagg cggattgata
     1981 cttttttatt ggaggtttca acagcatgtg caatggcaac ttcggcggcg tatcgaaagc
     2041 cgttgccacg ggttttcgtg cactcaaaaa catcaatctg gcaatcaccc gcggagaaac
     2101 caaagacatc tttaaagccg tcaaagcaca ggggccggca gcacaccgag caattgctcg
     2161 actgcgacgc cgattgccag cagccgccgg tcgtcgccga gcggtccatc cagttccagg
     2221 ccgaccggca agccgctggc gctcatcccc gccgccagcg acaaacccgg aatgccggac
     2281 gtgctggccg gatccgtatt gcgcaggaac gcgtccatcg tatcgatcga cgcggcgcca
     2341 tcgatcgata ccgtcgacga gccgttcagg tcgtcgatct gcaccgcgac gagacgcgtg
     2401 gtcgggaaca gcagcgcgtc gagccgctca tcggcgaatg tcgccgcgac ataccgctgc
     2461 aagcgcggcc gccattgctt cagcgccgcg tcgtaatggg cgccgagtac gtcggcgagc
     2521 acgccgtcgt agatcgcgcg cacgtccggg ctagcgatgc gggtcgccag ttcggcgacg
     2581 gtcctgacgg gcgcatcgtt ggcgacgagc caggcgagca cgtcctcgcg cggctcgtgg
     2641 atcgcgagcg ggccgcctac ctggccgttc aaggtttcca gctcgctcat cgcgacgggc
     2701 acgaaggtga cgcccgcggc ttcgagccgg cgcaaggcgg cgcgcgcgac gtcttccacc
     2761 tgcctttcga gtccttccca gagcggggcg ggcaagccga tacgcaagcc gctcagcgtg
     2821 agcgcgggca gcggtccggc accggtgatc acgccgtcga gcaaggcaat atcggcgacc
     2881 gtgcgcgcca tcggacctac cgtgtcgcgt gtatggctga tcggcacgac agcattcgga
     2941 tcgtaatagc gccgctcggc gccgccgttg cccaccgacg gacgaaaccc cgcggtgccc
     3001 gtcaacgcgg ccgggatgcg ggcggagccg ccggtgtcgg tgccgagacc ggcgggcacg
     3061 atgcgcgcgg cgatcgccac ggcggtgccc cccgacgagc cgccggggat cagcgagggg
     3121 tcgtacggat tgcgcaccgg cccggcgtgc acggccaggt tcgtgctggt gatgccgaac
     3181 gccagttcgt gcatgttggc tttgccgagc acgatcgcgc cggcatcgag cagcc
//
LOCUS       DATASH010000937         2365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5106437,
            whole genome shotgun sequence.
ACCESSION   DATASH010000937 DATASH010000000
VERSION     DATASH010000937.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5106437"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..156)
                     /locus_tag="VHV99_25050"
     CDS             complement(<1..156)
                     /locus_tag="VHV99_25050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3638690.1"
                     /translation="MSRRVLVTGASRGIGRAIAYQLAADGFAVSVHCRTGRAEAEAVA
                     TGIAAQGG"
     gene            complement(153..632)
                     /locus_tag="VHV99_25055"
     CDS             complement(153..632)
                     /locus_tag="VHV99_25055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007744135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog family protein"
                     /protein_id="HEX3638691.1"
                     /translation="MNNNIFGSIETILPHRGTMLLLDGVSACTDETATAYASVRADAW
                     YANEDGAMPAWIGIELMAQTIAAHVALLAMRAGGRARPGVLLGTRRYEVHVPVFARGA
                     RLRIEAKELLRGEEGHGAYECSIHHDQDGRTVCCAEAVIKVYQPADFQSFILGSFGS"
     gene            complement(650..1834)
                     /locus_tag="VHV99_25060"
     CDS             complement(650..1834)
                     /locus_tag="VHV99_25060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEX3638692.1"
                     /translation="MNGPRVYLHALGMINALGDSMDTIVPALAAGIAPGMRTIHTGIG
                     DTFAGSVTAPLNLAPPDSLSRYDCRNNRLLLAALAQIAPAVEAARERYGAHRIGVVLG
                     TSTSGIEAAEAAFVYQAQAGKLPVSFDYAQVEIGAVAPFAAAALDLHGPAFTISTACT
                     SSAKAFVSARRLLQLNLCDAVVVGGIDSLCELTVQGFASLESTSSTRSNPMSRNRCGI
                     NVGEGAAVFLMSRDEGAVVLAGVGESSDAHHISAPDPLGAGGEIALRAALADAGLVPA
                     DIGYVNLHATATRKNDDMEAHLMARVFPHGVAASGTKPLTGHQLGAAGATELGFAWLT
                     LARDGVALPRHLWDGEADPALPVLDLVGSERFLSRTVGARYVMSNSFAFGGSNVSLIL
                     GR"
     gene            complement(1831..2365)
                     /locus_tag="VHV99_25065"
                     /pseudo
     CDS             complement(1831..2365)
                     /locus_tag="VHV99_25065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017778077.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
BASE COUNT          475 a          788 c          705 g          397 t
ORIGIN      
        1 gccgccctgc gcggcaatgc cagtcgcgac cgcttccgct tcagcgcgtc cggtgcggca
       61 atgcacggag actgcgaagc cgtctgccgc gagttgatac gctatcgcgc ggccgatgcc
      121 acggcttgcg cccgttacaa gtacacgccg gctcatgatc cgaaactccc aagaataaat
      181 gactgaaagt cagccggttg atacaccttg atgactgcct cggcacagca cacagtgcgg
      241 ccatcctggt cgtgatggat cgagcattca tacgcgccat ggccttcttc gccacgcagc
      301 agttctttcg cttcgatgcg caagcgggcg cctcgcgcga ataccggtac atgtacctcg
      361 tatcggcgcg ttccgagcag gacgccggga cgtgcccgac cgcctgcgcg catcgcgagt
      421 aaagctacgt gcgcggcgat tgtctgcgcc atcaattcaa tgccgatcca cgccggcatc
      481 gcaccgtcct cgttggcgta ccaggcatcc gcgcgcacgc tcgcgtaagc cgttgcggtt
      541 tcgtccgtgc aggcgctgac gccgtcgagc aacagcatcg tgccacggtg cggcaagatg
      601 gtttcaatgg agccaaagat gttgttattc atagaggtcg tcacagtcgt caacggccga
      661 gtatcaggct gacgttgctg ccgccaaagg caaacgagtt gctcatcaca taccgcgcgc
      721 ccactgtgcg cgacagaaac cgctcgctgc cgacgagatc aagcaccggc aacgccgggt
      781 cggcctcgcc gtcccacaga tgacgcggca aagcgacacc gtcgcgcgcc agcgtgagcc
      841 acgcgaagcc cagttcggtc gcccccgcgg cgccaagttg atgcccggtc agcggtttgg
      901 tgccgctcgc cgcgacgccg tgcgggaaca cccgcgccat gagatgcgct tccatgtcgt
      961 cgttcttgcg cgtcgccgtt gcatggagat tcacatagcc gatatcagcc ggcacaagac
     1021 cggcatccgc caatgcggcg cgcaatgcga tttcaccgcc cgcgccgagt ggatcgggtg
     1081 cagaaatgtg atgcgcatcg cttgattcgc ccacgcccgc cagtaccacc gcaccttcat
     1141 cgcgactcat caggaacaca gccgcgccct cgcccacgtt gatcccgcag cgattgcggc
     1201 tcatcggatt ggagcgtgtc gaactggtcg attcgagcga cgcaaagccc tgcacggtca
     1261 gttcgcataa cgaatcgata ccgccgacca cgaccgcgtc gcataggttc aactggagca
     1321 accgacgcgc ggaaacgaaa gccttggcgc tcgatgtgca cgcggtcgag atcgtgaatg
     1381 ccgggccgtg aaggtcgagc gctgcggcgg cgaacggcgc aaccgcgcca atctccactt
     1441 gggcgtagtc gaagctcacc ggcaatttac ctgcttgcgc ctggtacaca aacgcagcct
     1501 cggccgcttc aatacccgat gtactcgtgc caagcaccac gccaatccga tgcgcgccat
     1561 aacgctcgcg agcggcttct accgccggtg cgatctgcgc gagcgcagca agcagcaggc
     1621 gattgttgcg gcagtcgtaa cgcgaaagtg agtccggagg cgcaagattg agcggcgcgg
     1681 tcacgctgcc ggcaaacgta tcgccaatgc cggtgtggat cgttctcatg cccggcgcaa
     1741 tgccggccgc gagcgccggc acgatcgtgt ccatgctatc gccgagcgca ttgatcatcc
     1801 ccagcgcatg cagataaacc cgcggcccgt tcatgcggcc ctccttcgtt ccgatggcat
     1861 gcctaccggc gcaagcagca ccgataccgc gataccaagt gcgagcgtcg cgccgaaact
     1921 cctcagtgcg ggcatcgcgc tcatggcgag cagaccaaat gacagcaggg ttgttgccgc
     1981 cgacagcagc acgcctgtcc acactgcgcc aagattggcg tccgcgcgtg cggcgccttc
     2041 gcgcaggaac acggcatagt ttgcgcctac cccgagtacg agcattaacg cgagccagtt
     2101 aaacagattc agcggcacgc ctgcatagcc gaatacggcg agcgtcacgc cgaccgcgag
     2161 caagacgggc agcgtgacga caatgccgcc aagcggccgg tagcgcgcca tcatcagcaa
     2221 gagcacgctt agcagcgcgc ctgcgagcca gaggccgcta tcgacacgat aagcgccgaa
     2281 cagcgtctat acgctcaagg ctttatcgac gaaggttacg cccggtaatg catgtgctgt
     2341 ggatatcagc acgggttcat ttgcg
//
LOCUS       DATASH010000938         3559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_160556,
            whole genome shotgun sequence.
ACCESSION   DATASH010000938 DATASH010000000
VERSION     DATASH010000938.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160556"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VHV99_25070"
     CDS             <1..286
                     /locus_tag="VHV99_25070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX3638693.1"
                     /translation="ALAAARDAAPLLQRGMLFEEVPADWLRIVRELDCVSLHASHRYL
                     TEPLVAEIKATGLRVLAYTVNDPERARLLALWGVDMICTDRIDRLEHAMF"
     gene            492..1604
                     /locus_tag="VHV99_25075"
     CDS             492..1604
                     /locus_tag="VHV99_25075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3638694.1"
                     /translation="MKRTALARLLSMSAAAGVAAAGVNSASAAPLDALVAAAKQEGQL
                     TVIALPHDWCNYGELVAGFQKKYGIKINELNPDAGSGDEVEAIKANKGNKGPQAPDVI
                     DVGLSFGPSAKAEGLLQPYKVASWNSIPKESKDADGYWYGDYYGVLAFEVNADMIDKA
                     PSDWSDLLKSDYKNAVSLAGDPRSANQAIQAVFAAGLSQSKGDVSKADKAGLKYFADL
                     NKSGNFVPVIGKAASLAQGTTPIVVRWDYNALADRDTLKGNPKVQVVIPKTGVVAGVY
                     VQAISAYAPHPNAAKLWMEYLYSDEGQIGWLTGYCHPMRYNELVAQKKVPQALLDKLP
                     PPSSYKNVVFPTLSQQDAAKDTITKHWDEAVGANVK"
     gene            1851..2789
                     /locus_tag="VHV99_25080"
     CDS             1851..2789
                     /locus_tag="VHV99_25080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX3638695.1"
                     /translation="MSASSDAGSVQPELLVPPVPTPTPRVDGPGRASQFLAWIGVVPF
                     FTFALLFLILPTGFLMVGAFQDSQGHFTLANFRDLLQPTVMDAYWISFKVSAASALGG
                     ALIGSLLAWAAVQGKLPGWIRPTLMTFSGVASNFAGVPLAFAFICTLGRVGLVTVLLK
                     KYLGFNLYSTGFSVLSFSGLTLTYLYFQIPLMVLIVTPALDGLKREWREACDCLGGTA
                     FQYWRHVALPVLWPSVLGATLLLFANSFGAVATAYALTGSSLNIVTILLYAQIRGDVM
                     HNANLGYALALGMVLVTGLSNGGYIWLRSRAERGRR"
     gene            2786..>3559
                     /locus_tag="VHV99_25085"
     CDS             2786..>3559
                     /locus_tag="VHV99_25085"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3638696.1"
                     /translation="MKGQSRVGAWTAMIVGSLYFLIPLLATFEFSLRMKRGVYSFEAY
                     SVVLSDPRFQASFGYSILMAVLTIIVGVLLVVPTAYYVQLRLPKLRPVVEFITLLPLV
                     VPAIVIVFGYLRIYNSSSILPLTGNERATDLLLVFGYVTLSLPYMYRAVDAGLRAVDV
                     RSLTEAAECLGANWPTILFKVIFPNIRSGILSGAFLTFAVVIGEFTLASLLDRPAFGP
                     YLQLIGANRAYEPSALAISAFVMTWASMGLIQVFGSARAL"
BASE COUNT          625 a         1039 c         1156 g          739 t
ORIGIN      
        1 ggcgctcgca gcggcgcgcg atgcggcgcc cctgctgcaa cgcggcatgc tgttcgaaga
       61 agtgccggcc gactggctgc gcatcgtgcg ggaactggac tgcgtgtcat tgcatgcgag
      121 ccaccggtat ttgaccgagc ctctggtcgc tgagatcaag gcgaccgggt tgcgcgtgct
      181 ggcctacacg gtgaacgatc ctgagcgggc gcggctgctc gcactatggg gcgtcgacat
      241 gatttgtacg gatcgcatcg acagactgga acacgcgatg ttttgagcgt tgaggaacgg
      301 gaacccggcg cacaccacgg gttgagcccc ggagaacccg gaccgggcac tcgccatgtt
      361 ttaggcgccg gtgaacccgg accgagcatg gcgcgggtgc gcgaatgcgc gcttgcacgg
      421 gcgtgtgcca atagagctag aaactagaaa ttttccaaac gtagcaattt cggcaaagga
      481 tggggaagac tatgaagcgc accgcgttag ctcgtttact ttcgatgtcg gccgctgcgg
      541 gtgtcgccgc agccggcgtg aacagcgctt cggccgcgcc gctcgacgca ctggtcgcag
      601 ccgccaaaca ggaaggccaa ctcaccgtga tcgcgttgcc gcacgactgg tgcaactatg
      661 gtgaactggt ggccggcttt cagaagaagt acggcatcaa gatcaacgag ctgaatccgg
      721 atgcgggttc gggcgacgaa gtcgaagcca tcaaggcgaa caagggcaat aagggtccgc
      781 aagcgcccga cgtaatcgac gtgggtctgt cgttcggtcc gagcgcgaag gccgagggtc
      841 tgctgcagcc gtacaaggtc gcgtcgtgga actcgattcc gaaagagtcg aaagatgccg
      901 atggctattg gtatggcgat tactacggcg tgctcgcgtt tgaagtgaac gccgacatga
      961 tcgacaaggc gccgtccgac tggagcgatc tgctcaagtc agactacaaa aatgccgtct
     1021 cgctggccgg cgacccgcgt tcggccaatc aggcgatcca ggccgtgttt gccgcgggcc
     1081 tctcgcaaag caagggcgat gtaagcaagg cggacaaggc cggcctcaaa tacttcgctg
     1141 atctgaacaa gagcggcaac ttcgtgccgg tgatcggcaa ggccgcgtcg ctcgcgcagg
     1201 gcaccacgcc gatcgtcgtg cgttgggact acaacgcgct ggccgaccgc gacacgctca
     1261 agggcaatcc gaaggtgcag gtggtgattc cgaaaacggg cgtggtcgcc ggtgtgtacg
     1321 ttcaggcaat cagcgcttat gcgccgcatc cgaatgccgc caagctctgg atggaatacc
     1381 tgtactccga cgaaggacag atcggctggc tgaccggcta ttgccatccg atgcgctata
     1441 acgaactggt ggcccagaag aaggtgccgc aggcgctgct cgacaagctg ccgccgccgt
     1501 cgtcgtacaa aaacgtggtg ttcccgacac tgtcccaaca ggacgccgcc aaagacacca
     1561 tcacgaagca ttgggacgaa gcggtcggcg cgaacgtcaa gtagtcggcg tggcgcatgt
     1621 ccgcgaacgg atcgggctcg tcgcgcgagc tgccacggcg acgaatttgc gactgcgtcc
     1681 agccacgatt gatgagcaaa agcggttgcg gccgcagcgg agcagcgagc atttgcaagc
     1741 tgctgcacaa gccgtggcgt ctagcagcga cgtgtagttt caacggtagc gcgtcgctca
     1801 actagagcgg cgcgcctggc gttaggcgca aaaagccgca agggtgagct atgagtgctt
     1861 catcggatgc cggatcggtg cagccggaat tactggttcc gcctgtgccc acgccaacgc
     1921 ctcgcgtcga cggtccgggc cgcgcgtcgc agttccttgc gtggatcggc gtggtgccgt
     1981 tttttacctt cgcgttgttg tttctgattc tgccgaccgg ctttctgatg gtcggcgcat
     2041 ttcaggattc gcaaggccac ttcacgctcg ccaatttccg tgatctgctg cagcccacgg
     2101 tgatggacgc ctactggatc agcttcaagg taagcgctgc ctccgcgctc ggcggcgcat
     2161 tgatcggctc gctgctcgcg tgggcggcgg tgcaaggcaa actgccgggc tggattcgtc
     2221 ctacgttgat gacgttctcc ggcgtcgctt cgaatttcgc cggcgtgcca cttgcattcg
     2281 ccttcatctg cacgcttggg cgcgtcggtc tcgtgaccgt attgctgaag aagtacctcg
     2341 gcttcaatct gtactcgacg ggcttcagcg tcttgagctt ttcggggttg acgctgacct
     2401 acctgtactt ccagattcca ttgatggtgc tgatcgtcac gcccgcgctc gacggcttga
     2461 agcgcgaatg gcgtgaagcc tgcgactgcc tcggcggcac ggccttccaa tactggcgcc
     2521 acgtggcgtt gccggtgttg tggccgagcg tgctcggcgc gacgctgctg ctgttcgcca
     2581 attcgttcgg cgcggtcgcc acggcttatg cgctgaccgg cagttcgctg aacatcgtca
     2641 cgatcctgct gtacgcgcag attcgcggcg acgtgatgca caacgcgaat ctcggctacg
     2701 cgctcgccct cggcatggtg ctcgtgacgg gtctgtcgaa tggtggctat atctggttgc
     2761 gttcacgcgc tgaaaggggg cgtcgatgaa aggccaatcg cgtgtgggcg cgtggaccgc
     2821 gatgattgtc ggttcgctgt actttctgat tccgttgctc gccacgttcg agttcagcct
     2881 gcgcatgaag cgcggcgtct atagcttcga agcgtatagc gtcgtgctga gcgatccgcg
     2941 gtttcaggcc tcgttcggct attcgatcct gatggcggtg ctgacgatta tcgtcggcgt
     3001 gttgctggtg gtgccgaccg cgtactacgt gcagttgcgg ttgccgaagc tgcggccggt
     3061 cgtcgaattc atcacgctgt tgccgctggt ggtgccggcg atcgtgatcg tgttcggtta
     3121 tctgcgcatc tataacagca gctcgattct gccgctcacc ggcaacgaac gcgcgaccga
     3181 cctgttactg gtgttcggtt acgtgacgct ctcgctgccc tatatgtatc gcgcggtcga
     3241 tgccggtttg cgcgcggtcg acgtgcgttc gctgaccgaa gccgccgaat gtctcggtgc
     3301 gaactggccg acgattctgt tcaaggtgat cttcccgaat atccgctcgg gcattctgtc
     3361 cggcgcgttt ctcacctttg cggtggtgat cggcgagttc acgctggcaa gtctgctcga
     3421 ccgccccgcg ttcggcccgt atctgcaatt gatcggagcg aaccgcgcgt atgaaccgtc
     3481 ggcgctcgcg atcagcgcgt ttgtgatgac gtgggcgtcg atgggcttga ttcaggtatt
     3541 cggctcggcc cgcgcgctt
//
LOCUS       DATASH010000939         5747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3521619,
            whole genome shotgun sequence.
ACCESSION   DATASH010000939 DATASH010000000
VERSION     DATASH010000939.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5747
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3521619"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(376..1152)
                     /locus_tag="VHV99_25090"
     CDS             complement(376..1152)
                     /locus_tag="VHV99_25090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006052429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3638697.1"
                     /translation="MGRSINLEGKVALITGASSGLGKRFAQVLSQAGAKVVLASRRTE
                     RLKELRAEIEASGGAAHVVSLDVTDYQSIKSAVAHAETEAGTIDILVNNSGVSTTQKL
                     SEVTPADFEYVFDTNTRGAFFVAQEVAKRMIMRGNNAQKPSYRIINIASMAGLRVLPQ
                     IGLYSMSKAAVVHMTKAMALEWGKHGINVNAICPGYIDTEINHHHWSTEQGQKLVSLL
                     PRHRVGTPEDLDGLLLLLAADESQFINGSVIAADDGFGLA"
     gene            1341..3014
                     /locus_tag="VHV99_25095"
     CDS             1341..3014
                     /locus_tag="VHV99_25095"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEX3638698.1"
                     /translation="MTPASLIEQYGPRESMEYDVVIVGGGPAGLSAAIRLKQLAAEKS
                     VELGVCVLEKGSEIGAHILSGAVMDQRAITELIPDWKEKGAPLTVDVTEDKFLFLTET
                     GSKSVPVWALPDNFKNHGNYVVSLANVTRWLGQQAEALGVEIFPGFPAAEILYNDDGS
                     VKGVATGNLGIGKDGEPTENFQLGMELHAKYTLFCEGARGHLGRQLNDKFRLREGVDP
                     QVYGIGIKELWEIDPSKHKPGLVMHTAGWPLETDTYGGSFLYHMDNNQVVVGFVVGLG
                     YTNPYLSPFEEFQRYKTHPAIRAVLEGGKRVSYGARAITAGGLLSLPKLVFPGGALVG
                     DDAGFLNASRIKGSHAAIKTGMLAAEAAFDAVQAGRTSDELTAYPESFKTSWLHTELH
                     RARNFKQWMSKGLYLGTLMVGIEQKLFGGNVPWTLHHQHSDHEMLKPASQCKPIVYPK
                     PDGKLTFDRLSSVFISNTNHEENQPAHLTLKDPSVPVNVNWQTYAGPESRYCPAAVYE
                     FVKNDDGSERLVINAQNCVHCKTCDIKDPTQNIVWVTPEGGGGPNYPNM"
     gene            complement(3116..4591)
                     /locus_tag="VHV99_25100"
     CDS             complement(3116..4591)
                     /locus_tag="VHV99_25100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3638699.1"
                     /translation="MTDTTSEPADRQLAALRVRAAAPQAGAADCLVFGQTLLQRAGRD
                     KASQREALAALVRAYQLDPSGEPTLLHTIAQTAFIVRDWVLVDSAATLLLERNREDAS
                     ALIWRAAAVQQRNDFDTAERLLREAVRVVPGNPVALHKLALCVKEQARFAQAEALLRQ
                     VLVLSPNNAHAQFDLSELEIRTGRFAEGWAHYESRVAFGDDLNNAQHALAAISPHWWG
                     ESLEGKTLIVYGEQGNGDCLWAVRFLPRLAARARREGGRVIFGHDGPLRHLFERMLPA
                     DLPLETSLETRPDFHCGLMSLPLRLGVFDATGWGAPYLSAEPARVQAWCERVQAHTAG
                     KPGNRKVGLVWNGNPDHIRDRRRSVPVEQLESILNVSGVTYFALSPGRSETVALWRAK
                     GLDVVDLTHHFQSGFDDVAALLANLDLVVTIDSGPAHLAGALGVPTCLMIDHVSAWFW
                     GDESRTTPWYDSIELFRQPEIGAWAPVLAKVRERLEMLRSA"
     gene            complement(4646..5746)
                     /gene="aroC"
                     /locus_tag="VHV99_25105"
     CDS             complement(4646..5746)
                     /gene="aroC"
                     /locus_tag="VHV99_25105"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX3638700.1"
                     /translation="MSGNTLGTLFTVTTFGESHGPAIGCVIDGCPPGMALNEADIQLE
                     LDRRKPGTSRHVTQRQEEDKVEILSGVFEGQTTGAPIALLIRNTDQRSKDYGNIADTF
                     RPGHADYTYWQKFGIRDYRGGGRSSARLTAPTVAAGAVAKKWLREKFGTEIRGYMAAL
                     GEINVPFVDWAHVRENPFFVPNAEIVPELEDYMDALRKDGDSIGARINVVASGVPVGL
                     GEPLFDRLDADIAHAMMGINAVKGVEIGAGFASVAQRGSVHGDELTPEGFVGNHAGGV
                     LGGISTGQDITVSIAIKPTSSIRTPRRSIDKAGQPAVVETFGRHDPCVGIRATPIAES
                     MLALVLIDHALRNRAQCGDVAVSTPKIAASAP"
BASE COUNT         1153 a         1802 c         1759 g         1033 t
ORIGIN      
        1 gcgtccatgt cgccccagcg gatcggcatc gacatctcga aaactgcatg aaaatcgctc
       61 atcgcacttc tttaacggat tgaaatcagg tgaagccggc acgcgggccg gcgcagaaat
      121 gacaaacaaa ataacgcagg cctgccttca gctgaaggcc tgcgcgcgta aaacagtttt
      181 tcttcgactg tgtgccgatc cgcggcggtg gacgtgcgct gccgcccttc ggctacacgc
      241 aagacgccca cgccgagcga ggcggcgctg acccgaaggc gccacagctt cccgacggcg
      301 gcgcaaaagt cgcgtcacgc catccaaccg aggccgaatg cgccccagcg cggccccgag
      361 cggcccaaag agcgttcagg ccagcccgaa cccgtcgtcg gcggcaatca ccgagccgtt
      421 gatgaactgc gattcgtcgg ccgccagcag cagcaacagt ccgtccagat cctccggcgt
      481 gcccacccga tgacgcggca gcaacgacac cagcttctgc ccctgctcgg tcgaccagtg
      541 atggtgattg atctcagtat cgatatagcc cgggcagatg gcgttcacgt tgatgccgtg
      601 cttgccccac tccagcgcca tggccttggt catatggacg accgcggcct tgctcatcga
      661 atacaggccg atttgcggca gcacacgcaa acccgccatc gacgcgatgt tgatgatccg
      721 gtacgacggc ttctgcgcat tgttgccgcg catgatcatg cgcttggcga cttcctgggc
      781 gacaaagaag gcgccgcgcg tgttagtgtc gaacacatat tcgaagtcgg ccggcgtgac
      841 ttccgagagc ttctgcgtgg tcgacacgcc tgaattgttc accagaatgt cgatcgtgcc
      901 cgcctcggtt tccgcgtgag caacggcaga tttgatgctc tgataatccg tgacgtcgag
      961 cgagacaacg tgcgccgcgc cgccggaggc ttcgatctcg gcacgcagct ccttcagacg
     1021 ctcggtgcgg cggcttgcca gcacgacctt cgcgcctgcc tgcgacaata cctgagcaaa
     1081 gcgctttccc aacccgctcg aggcgcccgt gatcagcgcg accttgcctt ccagattgat
     1141 cgaacggccc attgtcgttc ccttgttcgg ttattgttac agacgcagtt gacgggtgcg
     1201 gtaagcccgt cactacgggt cataccgtat cacaaaaata gaacggtcgt gctaatctct
     1261 gctcatcggg ttgcagtgca agagaccatc gcccacaata cgatcccgaa aaaagcattc
     1321 taacggtaag aggagcatca atgacccccg caagtctcat tgagcagtac ggcccgcgcg
     1381 agtcgatgga atacgacgtg gtgatcgtcg gcggcggccc ggctgggctg tccgcagcga
     1441 tccgcctgaa gcagctggcg gctgaaaaaa gcgtcgagct tggcgtgtgc gtactcgaaa
     1501 aaggctcgga gatcggggct catatcctgt cgggggcggt gatggatcag cgcgccatca
     1561 ccgaactgat tccggactgg aaagaaaaag gcgcgccgct gacggtagac gtcacggagg
     1621 acaaattcct cttcctgacc gaaaccggct cgaagagcgt gccggtctgg gcgctgccgg
     1681 ataacttcaa gaaccacggc aattacgtgg tcagcctcgc gaatgtcacg cgctggctgg
     1741 gtcagcaggc tgaagcgctg ggtgtcgaaa tttttccggg ctttccggcc gcggagattc
     1801 tctacaacga cgatggctcg gttaaaggcg tcgccacggg caatctgggc atcggcaagg
     1861 atggcgagcc gactgagaat ttccagctcg gcatggaact gcacgcgaag tacacgctgt
     1921 tctgcgaagg cgcacgggga catctcggtc gccagttgaa cgataaattc aggctgcgcg
     1981 aaggcgtcga tccgcaggtc tacggtatcg gcatcaagga actgtgggaa atcgatccgt
     2041 cgaaacacaa gccgggtctg gtcatgcaca cggccggctg gccgctggag accgacacgt
     2101 acggcggctc gttcctgtat cacatggata acaaccaggt ggtggtgggt ttcgtcgtcg
     2161 gtcttggcta cacgaacccg tatctgtcgc cgttcgagga gtttcagcgc tacaagacgc
     2221 atccggccat ccgcgcggtg ctcgaaggcg gcaagcgggt gtcgtacggc gcgcgggcga
     2281 ttacggccgg cggcctgctg tcgctgccga agctggtgtt cccgggtggc gcgctggtgg
     2341 gtgacgatgc gggcttcctg aacgcatcgc ggatcaaggg ctcgcatgcg gcgatcaaga
     2401 ccggcatgct ggcggctgaa gcggcctttg atgccgtgca ggcaggccgc accagcgatg
     2461 aactcaccgc gtatccggag tcgttcaaga cctcgtggct gcacaccgaa ctgcatcgcg
     2521 cgcgcaattt caagcagtgg atgagcaagg gcctgtacct cggcaccttg atggtcggca
     2581 tcgagcagaa gctgttcggc ggcaatgtgc cgtggacgct gcatcaccag cattcggatc
     2641 acgagatgct gaaacctgcg tcgcagtgca agccgattgt ttatccgaag ccggatggga
     2701 agctgacgtt cgatcgtctc tcgtcggtgt tcatttcgaa caccaaccat gaagagaacc
     2761 agccggctca cctgacgttg aaagaccctt cggtgccggt gaatgtgaac tggcagacct
     2821 atgcgggacc ggagtcacgc tattgcccgg cggcggtgta cgagttcgtg aagaacgacg
     2881 acggcagcga acggctggtg atcaacgcgc agaactgtgt gcactgcaag acctgcgaca
     2941 tcaaggaccc gacccagaac atcgtctggg tcacgcctga aggtggcggc ggtccgaact
     3001 atccgaatat gtaatgcaca caagcgccgc ttccaagcgg cgttttttta tcggcgcgcg
     3061 atcgcaacgc gatgagtggg cggacgcagg agatgagatt tgcaatacaa aaaactcaag
     3121 cgctccgcag catttcaagc cgttctcgca ctttcgccag caccggcgcc cacgcgccaa
     3181 tctcaggctg tcgaaagagt tcaatagaat cgtaccaagg cgtggtgcgc gattcgtcgc
     3241 cccaaaacca tgccgacaca tgatcgatca tcaaacacgt gggcacgccg agcgctcccg
     3301 ccagatgcgc aggcccgcta tcgatcgtga cgaccagatc cagattcgcc agcaacgcag
     3361 cgacgtcgtc gaatccggac tgaaaatggt gggtcaagtc gacaacatcg agacccttgg
     3421 cccgccacag cgcgaccgtt tcactgcgcc caggcgacaa cgcaaaatag gtcacaccgg
     3481 acacattcaa tatcgactcg agttgctcga ccggcaccga gcgccgccta tcgcggatat
     3541 ggtcaggatt gccgttccac accaggccga ccttgcgatt gcccggcttg ccggctgtgt
     3601 gtgcctgaac ccgctcacac catgcctgta cacgcgcagg ctcggcgctc agatacggcg
     3661 caccccaccc tgttgcatcg aaaacaccaa gccgtaacgg caagctcatc aatccgcaat
     3721 ggaaatcagg ccgcgtttca aggcttgttt cgagcggcag gtcggcaggc agcatccgtt
     3781 cgaatagatg ccgcaacggc ccgtcgtgac cgaaaatcac ccggccgccc tcacgccgcg
     3841 cgcgcgcggc tagccgcggc agaaagcgca ccgcccaaag acagtcgcca ttgccttgct
     3901 cgccatacac gatcagcgtt ttaccttcca gcgattcgcc ccaccaatgc gggctgatcg
     3961 cggcgagcgc atgctgcgcg ttattcagat cgtcgccgaa cgcgacgcgt gattcgtaat
     4021 gcgcccagcc ctcggcgaac cgtcctgtgc ggatttcgag ttcagacaga tcgaactgcg
     4081 catgcgcgtt attcggcgac agcacaagca cctgcctgag caatgcttcc gcctgcgcga
     4141 aacgcgcctg ctccttcacg cacaacgcga gcttgtgaag cgccaccgga ttgccgggca
     4201 cgacacgcac cgcttcgcgc aacagccgct cggccgtgtc gaagtcgttg cgctgttgca
     4261 cggcggcggc gcgccagatc aacgcgctgg cgtcttcgcg gttccgttcg agaagcagcg
     4321 tcgcggcgga atcgacgagc acccagtcgc gcacgatgaa cgccgtctgc gcaatcgtgt
     4381 gaagcaatgt gggttcgccc gaagggtcga gctgataagc gcgcaccagc gcggcaagcg
     4441 cctcgcgctg gctggctttg tcacggccgg cgcgttgcag cagcgtttgt ccgaacacga
     4501 gacagtcggc tgcaccggct tgcggcgcgg cggcgcgcac gcgcagtgcc gcgagttggc
     4561 gatctgccgg ctcggaggtc gtatcggtca tggagacgat gccacggcgc gcggtgcagg
     4621 tacgaaggcg ctcggagaac ccgctttaag gcgcgctcgc cgcgatcttc ggtgtgctca
     4681 cggcgacatc gccgcattgc gcacggttac gcaatgcgtg gtcgatcaac accagcgcga
     4741 gcatggattc ggcgatcggc gtggcgcgga tgccgacgca cgggtcatgc cgcccaaaag
     4801 tttcgacgac agccggctgc cctgccttgt cgatcgagcg gcgcggcgtg cgaatgctcg
     4861 aagtcggctt gatcgcgatg gacaccgtga tgtcctgccc ggtcgaaata ccaccaagca
     4921 ccccgcccgc gtgattgccg acgaagccct ccggcgtcaa ttcgtcgcca tgcaccgagc
     4981 cgcgttgcgc gacactggcg aagcccgcgc cgatctcgac gcccttcacc gcgttgatgc
     5041 ccatcatggc atgggcaatg tccgcatcga ggcggtcgaa cagcggctcg ccgagcccga
     5101 ccggcacccc ggaagccacg acattgatgc gtgcgccgat cgagtcaccg tccttgcgca
     5161 acgcgtccat ataatcttcg agttccggca cgatctccgc attcggcacg aagaacgggt
     5221 tctcacgcac gtgcgcccag tcgacgaacg ggacattgat ctcgccgagc gccgccatgt
     5281 agccacgaat ctctgtaccg aacttttcgc gcagccattt cttcgccacc gcgccggctg
     5341 ccacggtcgg cgcagtcaaa cgggccgacg aacggccgcc gccgcgatag tcgcgaatgc
     5401 cgaatttttg ccaataggtg tagtcggcgt gaccgggacg gaaggtgtcc gcgatgttgc
     5461 cgtagtcctt actgcgctga tcggtattgc ggatcagcag cgcgatcggc gcaccggtgg
     5521 tctggccttc gaacacgccc gagaggatct cgaccttgtc ttcttcctga cgttgcgtga
     5581 cgtgacgcga cgtgccgggt ttgcggcggt cgagttcaag ctggatatcg gcttcgttaa
     5641 gcgccatgcc gggcgggcag ccgtcgatca cgcagccgat agcggggccg tgcgattcgc
     5701 cgaaggtcgt gacagtgaaa agcgtaccga gcgtgttgcc ggacatg
//
LOCUS       DATASH010000940         4320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1744369,
            whole genome shotgun sequence.
ACCESSION   DATASH010000940 DATASH010000000
VERSION     DATASH010000940.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1744369"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1285)
                     /locus_tag="VHV99_25110"
     CDS             complement(<1..1285)
                     /locus_tag="VHV99_25110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HEX3638701.1"
                     /translation="MEKSLLKNIAINFAGLILPVFVSLATVPAYIHGMGIERYGVINL
                     VWALIGYFSVLDLGISMATENHIAKARQSNDGTIQRIFWSAFFLNLATGVVGAVLIWF
                     GAYVYVVHIATIEPVFQREVLHALPWIALAVPIANISWVFSGAIGGMEKFALFNTNQT
                     IGTFLFQLLPLGALYLISPSLAVVVPAAVIARFLAALLLGYGTFRAIGIKRISKPEWP
                     LIRELFNYGRWLLLFSGASMIATTLDRVVIGSMLGAKYVAYYATPQNLVTRMNLLPSA
                     MLRTLFPRFSASASDHADELSHQALAFLNCAFTPCVVVALFALAPFLNVWLGHEMAVQ
                     SAPVGRILVIGVWLAGQSSIIGVLIQAKANPAQVARVGWIGLPVFALVLWLSIRWYGL
                     LGAGVAVVAKAFFDYAAFLYFSKLKPRPILRNMAGH"
     gene            complement(1533..3026)
                     /locus_tag="VHV99_25115"
     CDS             complement(1533..3026)
                     /locus_tag="VHV99_25115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638702.1"
                     /translation="MLLAPVLSGRQYLRELRLLQQNWLETGGIHVTPPPADADYAEAV
                     GHRLIASSVAMLSKFDLLRDTRLAERAPRRLLVLDSYDPSRSKKLVEACSAAGADAQF
                     EAFDECAAFMQESGVTETPREAIARIVQWLGVAESTAPESTLAAVIVKSPETPLLIAD
                     GVRETPVIFDNGRLFGILCEPEEGTQSRRSVVFVNPGATHRIGDARISVTLARRLARQ
                     GFASLRFDVGTLGDSAAAVDKRPLSLLFSRAGCDDVVSAAAFLAERGFDDVSCFAVCS
                     GAYVCLHAAPASPHIRSLVLVNAPKFGFPEDPMSFAVNDSRPKTATSTLMRSFKTAGK
                     WKRVLRGQRRLTPIVKELARHLGARMKASVAYLAEQTIGVDSAGSRVRAMVRGLGERD
                     VKVRFVYGAGDPGMDEFAVFFGAFGRRMRKWRNVETVHRDSLDHACFSYSAREDMMAS
                     FLRFMQGESPQAARSGLGRTRLRALLPRFSARAGRPDEMHAKAMESR"
     gene            complement(3039..3443)
                     /locus_tag="VHV99_25120"
     CDS             complement(3039..3443)
                     /locus_tag="VHV99_25120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3638703.1"
                     /translation="MRRQLNNDAPSGRNIEMTPVIFDHCFGWLHEGQPHATRGVVLCQ
                     PFGREAMWVHKGWRVFAEALADSGMPTLRFDYAGTGDSAGESEDGEQVERWLQSIRSA
                     IACLKESTGVTQVVLCGLRLGATLAALVAAEQ"
     gene            3653..>4320
                     /locus_tag="VHV99_25125"
     CDS             3653..>4320
                     /locus_tag="VHV99_25125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX3638704.1"
                     /translation="MVTAVHQPSPSDTPRFSVCICTRNRPGDLARALRSVEASTYAAH
                     QIVVSDDSTDTLTREMMAADFPRVVFLEGPRRGLGANRNCALDAVTGTHVAFIDDDAT
                     LSADFLSQMADSLQHGGQHPEKLIITGYELNRGVPVYANATTFLGFQSRPYRTGDHHD
                     TVVINSAIFPAWLFKLIRFDEHLVYGYDEVDIVMRATLQGRCAVRLLPDVFNSHFPST
                     VNRD"
BASE COUNT          926 a         1377 c         1271 g          746 t
ORIGIN      
        1 gatgtccggc catgttgcgc agaatcggcc gcggcttgag cttcgagaaa taaaggaacg
       61 ccgcatagtc gaagaaggcc ttcgcaacca ccgccacgcc agcgcccagc aggccgtacc
      121 agcggatgct cagccacagc accagcgcga agacgggcaa accgatccag ccgacccggg
      181 cgacttgcgc cggattggcc ttggcctgga tcagcacgcc gatgatgctc gattgccccg
      241 cgagccatac gccaatcaca agaatgcgtc cgacaggggc ggattgaact gccatctcat
      301 ggccgagcca gacgttcagg aacggcgcca gcgcgaacaa cgcaaccacc acgcacggtg
      361 tgaacgcgca attcaggaac gcgagcgcct ggtgcgagag ttcgtcggcg tggtcgcttg
      421 cggaggctga aaaacgcggg aacagcgtac gcaacatggc gctcggcagc aggttcatgc
      481 gcgtgacgag gttttgcggc gtggcgtaat acgccacgta cttggcaccg agcatggaac
      541 cgatcaccac ccggtcgagg gttgtggcaa tcatgcttgc gccagagaac aacagcagcc
      601 agcgcccgta attaaagagt tcgcgaatca gcggccattc cggcttcgaa attcgcttga
      661 tgccgatcgc gcggaatgtg ccgtagccaa gcagcagggc cgctagaaac cgtgcaatga
      721 cggctgccgg cacgaccacc gccaacgacg gcgagatcag gtacagcgcg cccagcggca
      781 gcaactggaa caggaacgtg ccaatggtct ggttggtgtt gaacagcgca aatttctcca
      841 taccgccgat cgcgccgctg aacacccacg agatgttggc aatgggcacg gccagtgcaa
      901 tccacggaag cgcatgcagc acttcgcgct ggaacaccgg ttcgatggtc gcgatatgca
      961 cgacgtacac atatgcgcca aaccagatca gcactgcacc aacaacgccc gtcgccaggt
     1021 tcagaaagaa tgcactccag aagatgcgct ggatcgtgcc gtcgttggac tgacgcgcct
     1081 tggcgatgtg gttttcagtc gccatcgaaa tgccgagatc gagcacgctg aaataaccaa
     1141 tcaatgccca taccaggttg atgacgccgt agcgttcaat ccccatgccg tgaatgtagg
     1201 cgggcaccgt cgcgagagag acgaacacgg gcaggatgag cccggcgaaa ttgatcgcta
     1261 tgttcttgag aagacttttt tccattgagc aacgggccga ttacaacaaa cacacacagg
     1321 atgaatcaag gcaccgcgca cagctttgcg gtgaatgcag gcgcgttgca gcgcacgggt
     1381 gatgcacgtt catgtgcacg cagcgggtgc ctgggccgaa ggtcagacga attacacgcc
     1441 agagtaaagg cgcgtcacat cagggtctgt acgcagacga acgcattgcg ccgcggcctc
     1501 agatctcttg caattgctta cattctttcg gttcaccgcg attccatcgc tttggcatgc
     1561 atctcgtccg gccggccggc acgcgcgctg aaacgcggca gtaatgcacg caggcgtgtc
     1621 cggccaagac cactacgcgc ggcttgcggc gactctccct gcatgaatcg aaggaacgac
     1681 gccatcatgt cctcgcgcgc cgaatacgaa aagcacgcgt gatcgaggga atcgcgatgg
     1741 acggtttcca cgttgcgcca cttgcgcatc cgccgtccaa acgcgccaaa aaacacagcg
     1801 aactcgtcca tgcccggatc tcccgcgccg tagacgaagc gaaccttcac gtcgcgctcc
     1861 cctagtccgc gaaccatcgc gcgcacccgc gaacccgctg aatccacgcc aatcgtctgc
     1921 tcggccaaat aagccacact ggccttcatg cgcgcgccta aatgccgtgc cagttctttc
     1981 acgatcgggg tgaggcgccg ctggccacgc agcacacgct tccatttgcc cgccgtcttg
     2041 aaggagcgca tgagggtgct ggttgccgtc ttcggccggc tgtcgttcac ggcgaacgac
     2101 atcggatctt ccgggaaacc gaatttgggc gcgttcacaa gcaccagcga acgaatatgg
     2161 ggactggcag gcgccgcgtg caagcacacg taggcgccag agcagaccgc gaaacacgag
     2221 acatcgtcga aacctcgctc ggccaggaac gcggcggcgc tcaccacgtc gtcgcagccg
     2281 gcgcgcgaaa acagcaggga gagcggacgt ttgtcgacgg cggccgcgct gtcgccgagc
     2341 gtgccgacat cgaaacgcag cgacgcaaaa ccctggcgcg caagccggcg ggcgagcgtg
     2401 acggagatgc gcgcgtcgcc tatccgatga gtcgcacccg ggttgacgaa tacgaccgag
     2461 cggcgcgact gcgtgccctc ttccggctcg cacagaatgc cgaacaggcg gccgttgtcg
     2521 aagatcacgg gcgtttcgcg caccccgtcg gcaatgagga gcggagtttc aggcgacttg
     2581 acgatcaccg cggccagcgt agattcaggc gccgtcgact cagccacacc caaccactgg
     2641 acaatgcgcg caattgcctc tcgcggcgtc tcggtcaccc cggattcctg catgaatgcg
     2701 gcgcattcgt cgaaggcctc gaactgcgcg tccgctccgg cggcgctgca agcttcgacg
     2761 agtttcttcg agcgggacgg atcgtaagaa tcgagcacga gcaagcgccg cggcgctcgt
     2821 tcggccagcc gtgtgtcccg cagcagatcg aacttgctca gcatggcgac gctcgatgcg
     2881 atcaggcgat gccctactgc ctccgcatag tcggcatcgg ccggcggagg ggtgacatga
     2941 ataccgcctg tttcaagcca gttctgctgg agcagacgca gttcacgcaa gtattgccgg
     3001 cccgacaaca cgggggcgag cagcaccagt tgatcgacct attgctcggc cgccaccagc
     3061 gcggcgagcg tggcgcccag acgcaagccg cacaggacca cttgcgtgac cccggtcgat
     3121 tctttcaagc aggcgatcgc cgaacgaatg ctttgcagcc accgctcgac ctgttcaccg
     3181 tcctcagatt cgccagccga gtcgccggtg ccggcgtagt caaagcgaag cgtgggcata
     3241 cctgagtccg cgagggcttc ggcgaacacg cgccagccct tgtggaccca catcgcctcg
     3301 cggccaaaag gctggcaaag caccacgccg cgcgtggcat gtggctgacc ctcgtgcaac
     3361 cagccaaagc agtgatcgaa aattacaggc gtcatctcga tgttgcgccc cgaaggagcg
     3421 tcgttattaa gttgtctgcg cattacactt tcacaaggct gatgattccg tgcgcccgca
     3481 cacactgcac aagcgcaccc tgatatttcc gccatcgcga gcacttcccc gccaccgtta
     3541 ttcctccgat gttcgatgtc ccacagacgg ctttttcatt gccgagtcaa atagggcatc
     3601 tcgccgtgtc gtgcatgcgc ggcacagaac aagaaaatgc cggggaaaac acatggtcac
     3661 cgctgttcac caacccagtc cgtccgatac gccgcgcttc tcggtatgta tctgtacgcg
     3721 taatcgtcca ggggacctcg cccgggcgtt gcgttcggtc gaagcctcca cgtatgcggc
     3781 gcatcagatc gtagtgtcgg acgacagcac cgatacgctc acgcgtgaaa tgatggccgc
     3841 tgactttccg cgcgtcgtct tcctggaagg accccgccgc ggccttggcg cgaaccgcaa
     3901 ctgcgcgctt gacgcggtaa ccggcacgca cgtggcattc atcgacgatg acgccacgct
     3961 cagcgccgac ttcctgagtc agatggccga ctcgctgcag catggcgggc aacatccgga
     4021 aaaacttatt attacgggat acgaattaaa tcgcggtgtg cccgtttatg ccaacgcaac
     4081 cacattcctc ggtttccagt cacggcccta ccgtaccggc gatcaccacg acaccgtggt
     4141 catcaatagc gccatttttc ccgcgtggct cttcaagctg atccgcttcg acgagcatct
     4201 ggtctatggt tatgacgagg tcgatatcgt catgcgtgcc acgctgcagg gaaggtgcgc
     4261 ggtccggctt ctgccggacg tgttcaattc gcatttcccg tcgacggtga atcgcgacta
//
LOCUS       DATASH010000941         5009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6290585,
            whole genome shotgun sequence.
ACCESSION   DATASH010000941 DATASH010000000
VERSION     DATASH010000941.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5009
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6290585"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1091)
                     /gene="metG"
                     /locus_tag="VHV99_25130"
     CDS             complement(<1..1091)
                     /gene="metG"
                     /locus_tag="VHV99_25130"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008924243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX3638705.1"
                     /translation="MSAPATDLSASAPSGRRQVLVTSALPYANGQIHIGHLVEYIQTD
                     IWVRTLRMHGHEVYYVGADDTHGTPVMLRAEKEGLTPKQLIDRVWQEHKRDFDSFGIS
                     FDNYYSTDSEENRGLSESVYLALKDAGLIDARDIEQAYDPVKEMFLPDRFIKGECPKC
                     GAKDQYGDSCEVCGSTYLPTELVNPYSVVSGATPIRKTSTHYFFRLSDPRCENFLREW
                     VSGLAQPEATNKMREWLGDAGEAKLADWDISRDAPYFGFEIPGAPGKYFYVWLDAPVG
                     YYASFKNLAEKRGLDFDAWIRKGSTAEQYHFIGKDILYFHTLFWPAMLEFSGHRTPTN
                     VFAHGFLTVDGAKMSKSRGTFITAQSVIDT"
     gene            complement(1485..2138)
                     /locus_tag="VHV99_25135"
     CDS             complement(1485..2138)
                     /locus_tag="VHV99_25135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX3638706.1"
                     /translation="MNAKIMTRLAVFAVAGSLLAGCATQQGTDTAVGTGVGAATGAAI
                     GAIFGGGKGAAIGAGAGAAVGGITGYNWQAIHNKLSGATKGTGTQITEQPDGSLKLNI
                     PSSVTFDTNSYAIKPSFAPVLDQLTQTMQQNPELIAQVIGYTDSTGQPGYNQTLSVNR
                     AESVTGYLGQRGVAPQRLSAQGMGQNQPVADNNTEAGRAANRRVEIYLRATAQHTTQ"
     gene            2481..3569
                     /gene="apbC"
                     /locus_tag="VHV99_25140"
     CDS             2481..3569
                     /gene="apbC"
                     /locus_tag="VHV99_25140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015600266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HEX3638707.1"
                     /translation="MSIDRALVDAALAAVTDPNTGKTYAAARNIRNVAVEGDTVSLEV
                     VLGYPAKRQFDAIRAQFADVLKAVPGVLNARVEVSQQIAAHTVQRGVKLLPNVKNIVA
                     VASGKGGVGKSTTAVNLALALASEGASVGILDADIYGPSLPMMLGIVGRPESPDEKSM
                     NPMTGHGLQANSIGFLIEQDNPMVWRGPMATSALEQLLRQTNWQDLDYLIVDMPPGTG
                     DIQLTLSQRVPVTGAVIVTTPQDIALLDAKKGLKMFEKVGIPILGIVENMGMHICSNC
                     GHEEHIFGAGGGERMGKEYGVDVLGSLPLDIAIREQADSGNPTLVADPQGRIAEIYRS
                     IARKVAIHIAERARDMTSKFPSIVVQNT"
     gene            3700..4233
                     /locus_tag="VHV99_25145"
     CDS             3700..4233
                     /locus_tag="VHV99_25145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEX3638708.1"
                     /translation="MGKRIDGQAVHAFIVLAASSVLLCGCSAFLRPQEKRADAQLLPT
                     VGNQAHGLVTFIERSDGVQVTYNIAGMPPNSDHALQVHERGDCNAADGSSAGQVFSPA
                     AERLKAGARVEGDLGNIHADANGVATGFIVAPDVSLDGIRSVLQRAVLLHHDATDPYA
                     YPPHGAGPALACGLIRQ"
     gene            4377..4946
                     /gene="dcd"
                     /locus_tag="VHV99_25150"
     CDS             4377..4946
                     /gene="dcd"
                     /locus_tag="VHV99_25150"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEX3638709.1"
                     /translation="MTIKSDKWIRRMAAAHNMIEPFAPDQVRVSEDGRKIVSYGTSSY
                     GYDIRCADEFKIFTNINSTIVDPKNFDEKSFVDFKGDVCIIPPNSFALARTVEYFRIP
                     RSVLTVCLGKSTYARCGIIVNVTPFEPEWEGHVTLEFSNTTPLPAKIYANEGVAQVLF
                     FESDEICETSYADRGGKYQGQHGVTLPKT"
BASE COUNT          929 a         1511 c         1596 g          973 t
ORIGIN      
        1 gtgtcgatca cgctttgtgc ggtgatgaac gtgccgcgcg acttcgacat cttcgcgccg
       61 tccacggtca ggaagccgtg cgcgaacacg ttggtcggcg tgcggtggcc tgagaattcg
      121 agcatggccg gccagaacag cgtgtggaaa tacagaatgt ctttgccgat gaagtggtac
      181 tgctcggcgg tcgagccttt acgaatccac gcgtcgaaat cgaggccgcg cttttcggcg
      241 aggttcttga agctcgcgta atagccgacc ggcgcgtcca gccatacgta gaaatatttg
      301 cccggcgcgc ccggaatttc gaagccgaag tacggggcgt cgcgcgaaat gtcccagtcg
      361 gcaagcttgg cttcgccggc gtcgccgagc cattcgcgca tcttgttggt ggcttccggc
      421 tgcgccaggc cgctgaccca ctcgcgcagg aaattctcgc agcgcggatc ggacaggcgg
      481 aagaagtagt gcgtcgaggt cttacgaatc ggcgtggcgc cagaaacgac cgagtacgga
      541 ttgaccagtt cggtgggtag ataggttgaa ccgcatactt cgcagctgtc gccgtattga
      601 tccttcgcgc cgcacttcgg gcattcgccc ttgatgaagc ggtccggcag gaacatttcc
      661 ttgacggggt cgtatgcctg ttcgatgtcg cgcgcgtcga tcaggcccgc gtctttgagc
      721 gccaggtaga ccgattcgct caacccgcga ttctcttcgg aatcggtcga gtagtaattg
      781 tcgaacgaaa tcccgaagct gtcgaaatcg cgcttgtgct cttgccagac gcgatcgatc
      841 aactgcttcg gcgtcaggcc ttctttctcc gcgcgcagca tgaccggcgt gccgtgcgtg
      901 tcgtcggcgc ccacgtagta gacctcgtgc ccgtgcatcc gcaacgtccg gacccagata
      961 tcggtctgga tatattcgac cagatggcca atatggattt gcccattcgc gtacggaagg
     1021 gccgacgtga cgagaacctg gcgacggccc gacggcgcgc ttgcggagag atcggttgcg
     1081 ggtgctgaca taaggatggt gggagcctgc ggtgggacga aacgttgatt ctagcagggc
     1141 aggcgcccgg ggcggactgg gccgcctggt cgctgccggg ggacgatggc taccggctgg
     1201 agggtgccgg agcgggtgcg tttgaaaacg gcgtgaaaac cgggcgccca aaaaaaaccg
     1261 gggctgttaa ccccggagca acaacgacaa gaggagaatg gtgacgcggc ggcttcgcca
     1321 gccacgtacc gtaaagacag acagcggatt ttcggcagag ttcgaaattt tttcgctctc
     1381 gtgcgagatt ttttcggtct gcttgttacg gcttgccgga cgccggtgga aacagcgtca
     1441 agcgcttcgc gcccggttgc ttcttcctct ttcagcctgt gctgttattg cgtggtgtgc
     1501 tgagccgtgg cgcgcagata gatttccacg cgacgatttg cggcacggcc ggcttcggtg
     1561 ttgttgtcag caaccggttg gttctggccc atgccttgtg ccgacaggcg ttgcggcgcg
     1621 acgccgcgtt gccccagata gcccgtcacg ctttccgcgc ggttgaccga cagcgtctgg
     1681 ttgtaacccg gctgacccgt gctatccgta tagccgatca cctgcgcgat cagttccgga
     1741 ttctgctgca tggtctgcgt caactgatcc agcaccggcg cgaacgacgg cttgatcgcg
     1801 tagctgttgg tgtcgaacgt gaccgagctc gggatgttca gtttgagcga gccgtccggc
     1861 tgctcggtga tctgcgtgcc ggtgcccttg gtggcgcccg acagcttgtt gtgaatggct
     1921 tgccagttgt agccggtgat accgcccacc gctgcgccgg cgcccgcccc gatcgccgcg
     1981 cccttgccgc cgccgaagat cgccccgatc gccgcgccgg tggcggcgcc cacgccggtg
     2041 ccgacggccg tgtcagtgcc ctgttgggtt gcgcagccgg ccagcaacga accggcaacg
     2101 gcgaaaacag ccaggcgagt catgattttt gcattcattt tttgattcct ctcttgagtc
     2161 aaacgagtaa aacggtggaa gcggtgaaac tatccgccaa acatggcggc cgtaaagcgt
     2221 gttactcgac gttaggagaa tcctgcgtct tctcaggtcc cggtccgaca gtgaagaagg
     2281 gtcttccggc aaggcttggc gccagccact acaatgatga ctgacgtacc cagcgatgcg
     2341 accccttggt gcgctgcctg cccgaagggt gcacggcaca cctggcttta gcaatgctgc
     2401 ctaacaattt ttttcaaatt ccggcgcgtg gccgagacct tggattccgc cgcgccgcag
     2461 gtgttcctca cggagtgtca atgagtattg atcgggcttt ggtcgacgct gcccttgcag
     2521 ccgtcactga ccccaacaca ggcaagacgt acgcggctgc caggaacatc aggaacgtgg
     2581 ccgtggaggg tgacacggtt agcctggaag tggtactcgg ctatccggct aagcggcagt
     2641 tcgacgcgat ccgcgcgcag ttcgccgacg tgctgaaagc cgttcccggc gtgctgaacg
     2701 cgcgcgtcga ggtgtcccag cagatcgcgg cgcacacggt gcagcgcggc gtcaaactat
     2761 tgccgaatgt taaaaatatc gtcgccgtgg cgtcgggcaa gggcggcgtc ggcaagagca
     2821 cgaccgccgt gaatctcgca ctggcactgg cgagcgaagg tgcgtcggtc ggcattctcg
     2881 atgcggatat ttacggcccc tcgctgccga tgatgctcgg cattgtcggc cgtccggaat
     2941 cacccgacga aaagtcgatg aacccgatga ccggccacgg cttgcaggcc aactcgattg
     3001 gctttctgat cgagcaggac aacccgatgg tgtggcgcgg cccgatggcg acctcggcgc
     3061 tcgaacagct gctgcggcag accaactggc aagatctcga ctacctgatc gtcgacatgc
     3121 cgccgggcac cggcgacatt cagctgaccc tgtcgcagcg cgtgccggtg accggcgccg
     3181 tgatcgtcac gacgccgcag gacatcgcgc tgctcgacgc gaagaagggt ctcaagatgt
     3241 tcgagaaggt cggcattccg atcctcggca tcgtcgagaa catggggatg catatttgct
     3301 cgaattgcgg ccacgaagag catatcttcg gcgcgggcgg cggcgaacgg atgggcaagg
     3361 aatacggcgt cgacgtactc ggcagcctgc cgctcgatat cgcgatccgg gagcaggccg
     3421 actcgggcaa ccccaccctc gtggcggatc cgcagggccg tatcgctgag atctaccggt
     3481 cgatcgcgcg caaggttgcg atccatatcg ccgagcgggc tcgcgacatg acctcgaagt
     3541 ttcccagcat cgtcgtgcag aacacctagc agccattcgg atgaggggtc aaagcggggc
     3601 cagggcgtca atttgcgccg ggtctcgcgg tatcatgtcg acttcacagg ttcggctcgg
     3661 cggccgcagg ggcggccacc aggccggcaa gaggatcgca tgggaaaacg aatcgacggg
     3721 caggcggtgc atgcgttcat cgtcctggct gccagcagcg tgctgttatg cggctgtagc
     3781 gcgttcctga ggccacagga aaagcgtgcc gacgcgcagt tgctgcccac cgtgggcaat
     3841 caggcgcacg gcctcgtaac gttcatcgag cgttcggacg gcgtgcaggt cacctacaac
     3901 atcgcgggca tgccgcccaa tagcgaccat gccttgcaag tgcatgagcg cggcgattgc
     3961 aatgccgccg acggctccag cgcgggccag gtgttttcgc cggccgccga gcgtctgaaa
     4021 gccggcgccc gcgtcgaagg cgacctcggc aacatccacg cggacgcgaa cggcgtggcg
     4081 accggcttca tcgttgcgcc ggacgtgtcg ctcgacggta tccgttcggt gttgcaacgc
     4141 gcggtgctgt tgcatcacga cgcgaccgat ccttatgcgt atccgccgca tggtgcgggc
     4201 cccgcgctcg catgcggctt gattcgccag tgagggcggc gcaggcggcg ctcaggcaat
     4261 gtccgcagca gcaatctcac gatcgcgtaa aataagcgcc tttcgctgac gggcccggta
     4321 cttgccgggt tgttacccgt cacacatctg tcactcaagt cgagcagcgt tcacctatga
     4381 ctatcaaatc cgacaagtgg atccggcgca tggccgcggc gcacaacatg atcgagccgt
     4441 ttgcgcccga tcaggtccgc gtctccgaag acggccggaa gattgtcagc tacggcacgt
     4501 cgagctacgg ctacgacatc cgctgcgccg acgaattcaa gatcttcacg aacatcaact
     4561 cgaccatcgt cgatccgaag aactttgacg agaagtcgtt tgtcgacttc aagggcgatg
     4621 tctgcatcat cccgccgaat tcgttcgcgc tggcccgcac ggttgaatat ttccgtattc
     4681 cgcgcagcgt cctgaccgtg tgtctgggca agtccaccta tgcgcgctgc ggcatcatcg
     4741 tcaacgtgac gccgttcgag ccggaatggg aagggcacgt cacgctcgaa ttctcgaaca
     4801 cgacaccttt gcctgcgaaa atctacgcga acgaaggcgt cgcccaggtg ctgtttttcg
     4861 aaagcgacga gatttgtgaa acgtcgtacg cggatcgcgg cggcaaatat caaggtcagc
     4921 acggcgtcac gttgcccaag acgtgacgcc cggcagcact gacccaccag ctattcgggt
     4981 gaccgctgct cctgcaaggc gcagcggtc
//
LOCUS       DATASH010000942         2338 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4907569,
            whole genome shotgun sequence.
ACCESSION   DATASH010000942 DATASH010000000
VERSION     DATASH010000942.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4907569"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VHV99_25155"
     CDS             complement(<1..201)
                     /locus_tag="VHV99_25155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006400867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX3638710.1"
                     /translation="MHFITDADVRRLITMRDAIEELRLALEEHGRGQARIQPRVRTRG
                     DDIMLSTMGAILPNAGVCGAKVY"
     gene            complement(245..1237)
                     /locus_tag="VHV99_25160"
     CDS             complement(245..1237)
                     /locus_tag="VHV99_25160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEX3638711.1"
                     /translation="MTEKANIIVIGTGGTIAGQGKAANNTSAYVCSVRGIDDILSTIP
                     HACALANLRTEQLLQNGSENFSNEHLLAIGKRVSTLLAQHDVDGAVIAHGTDTIEETA
                     YFLHLTLKSSKPVVVVGSMRPPSAMSSDAALNLYDAIAVAAHPSSVGLGTLVFANDEI
                     HTARDVAKSNSFKIEAFRSPYGPLGFVIEGQPRYYRHPSRAHTLATPWSIETLGPLPQ
                     VDIVYAYGALAPEAVSAITARAQGLIYAGTGNGNVAAHLIEPLRDAVGRGVHVVRASR
                     TGSGIVLRNGAQPDDAYGWLTVEDQIPSKARILLMLALTQARDLQALQAVFERY"
     gene            complement(1295..>2338)
                     /locus_tag="VHV99_25165"
     CDS             complement(1295..>2338)
                     /locus_tag="VHV99_25165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006400865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3638712.1"
                     /translation="RNDSPVRFRPKLSTEQWRWCFQFLSACTREQSELTTRKLLSLSF
                     LSRTLLHELIAAEPSLDFDFVRSGKLVLHRDAGAMQSAVKLLEFQRTLGCEQQALSAN
                     ACVELEPTLAHLRPSLAGGIHTPSEDTADCQRFCKSLEALLRARGVQFLMNTSIDCLR
                     AESGGRVEAWSGGAPLASDHVVVASGAAAARLLKSVSIRVPVYPLKGYSLTFDLQPQT
                     AAPHISITDFSRKVVYARLGKRLRVAGIADIDGYSLEPDPTRLVTLRAEAAAMYPDIV
                     RSCPGSEWIGLRPATPRGTPIVGPTRYRNLWLNVGHGALGFTLATGSAALLAQWLANS
                     NGYELRSTFALSH"
BASE COUNT          429 a          685 c          769 g          455 t
ORIGIN      
        1 atacactttc gcgccacaca cgccggcgtt cggcagaatc gctcccatcg tgctgagcat
       61 gatgtcgtcg cccctggttc tgacgcgcgg ctgaatccgt gcctggcccc tgccgtgttc
      121 ttccagcgcg agacgcagtt cctcaatcgc gtcgcgcatc gtgatgaggc ggcgtacgtc
      181 ggcgtcggta atgaagtgca tgatgtgtgc ttttggtggg gtcggttgtg aaggagcgcg
      241 cgcttcagta gcgctcgaag acagcttgca acgcttgcaa gtcgcgggct tgtgtcagtg
      301 ccagcatcag aaggatgcgg gcttttgagg gaatctgatc ctcgacggtg agccagccgt
      361 aggcgtcgtc gggttgcgcg ccgttacgca acacgattcc gctgccagtg cgcgatgcgc
      421 gcacgacatg aacgcctcgc ccgacagcat cgcgcagcgg ctcgatgaga tgcgctgcga
      481 cattgccgtt gccggtgccc gcatagatca aaccttgcgc gcgggcggtg atcgcgctca
      541 ctgcttccgg tgcaagcgcg ccgtaggcgt agacgatgtc gacctggggc aatggcccga
      601 gcgtctcgat cgaccacggt gtcgcgagcg tatgggcacg cgatggatga cggtagtagc
      661 gtggctgacc ttcgatcacg aaaccaagcg ggccgtacgg tgagcggaac gcctcgatct
      721 tgaagctgtt gctcttcgca acgtctcgtg cggtgtggat ttcgtcgttg gcgaacacga
      781 gtgtgccgag gccaaccgac gatgggtgtg cagcgaccgc gatcgcatcg tagaggttca
      841 gcgccgcgtc ggaactcatt gccgacggcg ggcgcattga gccaaccacc acaaccggtt
      901 tggagctctt gagcgtgaga tgaaggaagt acgcggtctc ctcgatcgtg tcggttccgt
      961 gcgcgatcac agcgccatcg acgtcgtgtt gcgcgagcag tgtggacacg cgcttaccga
     1021 tcgcgagcag atgctcgtta ctgaaattct cggagccgtt ttgcagcaac tgctccgtgc
     1081 gcagattcgc cagcgcgcag gcatggggaa ttgtgctgag aatgtcatcg atgccacgca
     1141 ccgaacacac gtatgcggac gtgttgttgg cggctttccc ctgaccggca atcgtgccgc
     1201 ctgtgccgat aacgatgatg ttggcctttt ctgtcatgcg atgtcgatga tgttgggaga
     1261 gtaagcacgt cacgaagcgc atcgcctcgc gggttcaatg tgaaagcgca aatgtgctgc
     1321 gcagttcgta gccgttgctg tttgccaacc actgcgcgag cagtgccgct gaacccgtcg
     1381 caagcgtgaa gccgagcgcg ccatggccga cgttgagcca cagattgcgg tagcgcgtcg
     1441 gtccgacgat cggcgtgcca cgcggcgtcg cggggcgcaa cccgatccat tcgctacccg
     1501 gacaagatct gacgatgtcg ggatacattg cagccgcctc tgcccgcagc gtcacaaggc
     1561 gtgtgggatc gggttcgagc gaataaccgt cgatatcggc gatgcccgcg acgcgcagcc
     1621 gtttgccaag ccgcgcatac actactttgc ggctgaagtc ggtgatgctt atgtggggcg
     1681 cagccgtctg cggttgcagg tcgaacgtca ggctgtagcc tttgagcgga tagaccggca
     1741 cacggatgga aacagacttc agtaaccggg ccgcagccgc gcctgaggcg acgacgacat
     1801 gatcggatgc gagcggcgca ccgccgctcc aggcttcgac acggccgccc gattcagccc
     1861 tcagacaatc gatcgatgtg ttcatcagga actgcacgcc gcgagcgcgc agcagcgctt
     1921 cgaggctctt gcagaagcgt tggcaatcgg ccgtgtcctc gcttggcgta tggatgccgc
     1981 ccgcgagaga aggccgcagg tgcgcgagcg tgggttcgag ttcgacgcac gcgttggcgc
     2041 tcaatgcctg ctgttcgcag ccgagcgtac gctggaattc aagcagtttg accgcgctct
     2101 gcatcgcacc cgcgtcacga tgcagcacga gcttgccgga gcggacgaaa tcgaagtcga
     2161 gtgagggttc ggcggcgatc agttcgtgaa gcagcgtccg actcaggaac gacagtgaca
     2221 gcagcttgcg tgtcgtgagt tcgctctgtt cgcgcgtgca tgccgacaga aactgaaagc
     2281 accagcgcca ctgttcggtg ctcagcttcg ggcgaaagcg caccggcgaa tcgttgcg
//
LOCUS       DATASH010000943        13148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2928721,
            whole genome shotgun sequence.
ACCESSION   DATASH010000943 DATASH010000000
VERSION     DATASH010000943.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 13148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13148
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2928721"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..1124)
                     /locus_tag="VHV99_25170"
     CDS             complement(147..1124)
                     /locus_tag="VHV99_25170"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX3638713.1"
                     /translation="MKICIFGAGAIGGLMGVQLARAGADVSFIARGAHLAAMREHGAR
                     LIMDGETFSAPVRCTSDPAELGVQDVVIVTLKAHSLPGVVDAMQPLLGKHTAIVTGVN
                     GIPYWYFYQHGGKFAGTRLASIDPDGSQWNKLGPERAIGCVLYPAAEIVEPGVIKHVY
                     GKKFPIGEPSGERTPRIQQLHEIMQAAGFDAPIRDNIRDEIWLKLWGNLCFNPISALT
                     HATLDVLTSDPGTRAVSRTMMLEAKRIADQFGVHFRVDVEKRIDGAGAVGAHKTSTLV
                     DLENRRPMEIDPLLTVVQEMGRLVAEPTPTIDVVLALIKLRERMALQGD"
     gene            complement(1393..2013)
                     /locus_tag="VHV99_25175"
     CDS             complement(1393..2013)
                     /locus_tag="VHV99_25175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013341757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEX3638714.1"
                     /translation="MSITAWLFFLPACFAINLAPGPNNLLSINVAARHGFMTAFVGGT
                     GRLVAFALMLVLAATGLAVVLHASEWFFLAIKLAGAAYLIWLAIQLWRSDAPAIDTTR
                     QDDASLWRIARQECFVAAGNPKAILVFTAFLPQFVDIAKPMLPQFAVLGASFLVLEWV
                     AIALYSWAGMHLGKWLVRTRVRRWFNRCCGAFLAVIGASFLLVRRT"
     gene            complement(2164..2703)
                     /locus_tag="VHV99_25180"
     CDS             complement(2164..2703)
                     /locus_tag="VHV99_25180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638715.1"
                     /translation="MLKGCGVVALPLMLSACSWSWFGLNSPPRAATHSTGWLSVAPAR
                     DFAYMPARNGQVSPNRIENTAMTGIVLKNDINETVRNSIASSLQVAGFHLTEGRKVLS
                     GSIEKFTVDDERSPAFWTLKMRYVVTDATTQKVVFSTIKTVRQKSPKFTSNIIAVEDT
                     VKLSVDALISDPGFVKSVN"
     gene            complement(2943..3569)
                     /locus_tag="VHV99_25185"
     CDS             complement(2943..3569)
                     /locus_tag="VHV99_25185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEX3638716.1"
                     /translation="MHEARIFSSTEVFALPTYTLPAPLSAELEIRKSRFIAHAIPVAD
                     RDAAMVELRRLRDEHPGATHVCWALLAGGQSGMSDDGEPSGTAGRPILEVLRHHDLDG
                     VLAAVVRYYGGVKLGAGGLVRAYTDAIAAALLDAPRVERIAQVALIVEVSYADEAKVR
                     RWIEAEGHGLVDSVYGMLVRMTVRLPVNAVDDARLALVDMTQGKAGFF"
     gene            3717..4343
                     /locus_tag="VHV99_25190"
     CDS             3717..4343
                     /locus_tag="VHV99_25190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011355998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEX3638717.1"
                     /translation="MLDFSTLGTFVAVVLGLFLIPGPAVLLVLTRTVHGGRKAGILTG
                     LGIAVGDFIHTLGAAVGLSALLMTSALAFNAVKFVGAAYLVYLGVRALREKQASAHLP
                     EVAPVSASKAFFQAIPAEVLNPKTALFFLAFLPQFVRPEHGSTFLQFTTLGLIFVGMS
                     ALYTTLIVLTIRPLGKLVKRLTWLSRWQNKIIGVLFISLGLRVATQTR"
     gene            complement(4409..4777)
                     /locus_tag="VHV99_25195"
     CDS             complement(4409..4777)
                     /locus_tag="VHV99_25195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638718.1"
                     /translation="MRLPPFEPPTLAELRAWWRTRDEQAIQRLILEIQRQRLTLLELR
                     NLIDSGVQQARAADRTLVERGEPLMTLRIRIAQEVLRVGDIDDTRQMSRAEQERLAVR
                     TEGQMEYAREGRLRRQRRNI"
     gene            complement(4927..5607)
                     /locus_tag="VHV99_25200"
     CDS             complement(4927..5607)
                     /locus_tag="VHV99_25200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018438338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX3638719.1"
                     /translation="MPTSTKKETRPRGRPRAYDSDQALADARDVFWRSGYAGTSLDQL
                     SAATGMNRPSLYGAFGDKHALYLATLERYIEAGRLAMVHALSEDLPLPQALLRVYDGA
                     LAWYLPADGDPLGCFMIGTAVVEAVDDAEVRATLAEGLRTYDKAFEKRFKLAQATGEL
                     AADAQPPLLARIASAILHSLALRARAGDSRASLRATAVAGVALICGSAGASPGPATGK
                     ATAKGRGR"
     gene            5673..6311
                     /locus_tag="VHV99_25205"
     CDS             5673..6311
                     /locus_tag="VHV99_25205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009771184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HEX3638720.1"
                     /translation="MDLYFSPLACSLATRIALYEAGAQAGFIQVDTKSKRLSDGSDFY
                     PISPLGQVPVLRTDEGWLLSENTAILPYVADRYPAARLAPAAGTAQRARLQQWLGFIS
                     TELHKAVFVPLLDARAPEEVKRYVRDKASLRLGVLQDHLAHSEYLLDGFSVGDAYLVT
                     VLNWASYGGIDLSQWPTVAEYYKRLMRRPSVAKAAAEEIALFEEEVARRAKS"
     gene            complement(6334..6591)
                     /locus_tag="VHV99_25210"
     CDS             complement(6334..6591)
                     /locus_tag="VHV99_25210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2866 domain-containing protein"
                     /protein_id="HEX3638721.1"
                     /translation="MVEDTVFEHLRAMPGNEWVRQIHSCKVSDPLQPPWGRSYRLVEW
                     TMKHTPESSRRVVPAESTPLEIAQAVVSHIPGRRFCLHGDD"
     gene            6753..6887
                     /locus_tag="VHV99_25215"
                     /pseudo
     CDS             6753..6887
                     /locus_tag="VHV99_25215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591666.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
     gene            complement(6910..8694)
                     /locus_tag="VHV99_25220"
     CDS             complement(6910..8694)
                     /locus_tag="VHV99_25220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX3638722.1"
                     /translation="MIQKKKSQSHDPYAPVAKNPLLAARLPMWRSKLVVLLVFGAFAS
                     LAGRAFWVQVVNQDFYVDQGQRRYQRTIELDATRGHIVDRNGSMLAVSLATYEIWATP
                     RLLDEAAFAPLSRLLDLPLAELRRRLNGDKTFVLLKRQVNADTADHLNKLGLAGITQI
                     ADSKRFYPEGESAAHVVGFTDIEDNGQEGVELAANEQLLGVPGQREVIRDRLGRVVSE
                     TRPLVPAQNGDTIRLTIDRRIQQLAYAQLKDAVAKHHAEAGSVVVLDARNGEILALAN
                     YPSFDPNDRARLTGRQLRNRAVVDTFEPGSTIKPVVVALSIDEGKVRPQSVIDTAPGW
                     YKIGPAVIHDTSNHGAMTVAEAVQKSSNIALAKLALNLPAEKIWNKYQEYGLGLRPEL
                     TFPGVASGKVRPYKRWRPIEQATMAYGYGLSTSLLQIAQVYTAYAGDGTLHHVSLLRK
                     DTNADSNSDTSAAKVADRNADVGTATQAGDKGHAVTTPATARAIRSMLEMAAGPGGTG
                     RAAAVEGYRIGGKTGTARKQVGATYAKNRYRALFVGMAPMSDPRLIVAVMIDDPAGKA
                     FYGGTVAGPVFSGVTGGALQLLGVPPDA"
     gene            complement(8817..9440)
                     /locus_tag="VHV99_25225"
     CDS             complement(8817..9440)
                     /locus_tag="VHV99_25225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006029060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HEX3638723.1"
                     /translation="MLLHLIYLLAIVAEAMSGALMGMRRGMDRFGLCLVGTVTALGGG
                     TVRDVLLGHYPLAWISHPDYVLITIGAASIAAASAKWLRNWQRLFVTVDALGLIAFTV
                     IGCDVAATLDVSPTIVVLAGAITGVCGGMLRDLLCNQMPLVLREELYASIALGAGALY
                     VALQAWGVESGPASIVVLAGGFAVRMLAVRFRWQLKVFTAADSGGAS"
     gene            9900..10019
                     /locus_tag="VHV99_25230"
                     /pseudo
     CDS             9900..10019
                     /locus_tag="VHV99_25230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
     gene            complement(10040..11581)
                     /locus_tag="VHV99_25235"
     CDS             complement(10040..11581)
                     /locus_tag="VHV99_25235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX3638724.1"
                     /translation="MTLNKKLASMIAVLWTGLILIGGFGAWQNRASMISDRRDQLTSL
                     IDQANHIVNRYYTLAQQHTITEDDAKKQALESLAAMRYGKDGYISVNDSQPVMLMHPI
                     KQDMVGKNLAQYTDPAGNHLFVDIVNAGNHDGGGFVDYLWSKPGSDKPVAKTSFAVHF
                     APWDWYIVTGMYMDDVQRAFYANLLRWLAITVTLGAIATIVMLLVLRSIRRTLGGDLE
                     VAVEAAQLMARGNLATRVPVQANDTGSLLHALHTMQAGLVDTVSRVRLGTENINIGAT
                     EIAAGNTDLSQRTEEQAAALVETASSMDQMTVNVKQNADSALQAAQLAGQAADVATRG
                     SRVVDDVVRTMGEITSSSRQIGDIIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAV
                     VAAEVRSLAQRSATAAKEIKALIETSTHSVDAGASLVANAGSTMGEIVQSVRRVNEIL
                     EEISHASREQSAGIEQVNRAVGEMDQVTQQNAALVEQAAAAAHSLKDQVGVLREAISS
                     FALPA"
     gene            12934..>13148
                     /locus_tag="VHV99_25240"
     CDS             12934..>13148
                     /locus_tag="VHV99_25240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012217178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEX3638725.1"
                     /translation="MNAREPAFISASRSARLRQMLVSNELEFMMEAHNGLSARIVREA
                     GFKAIWASGLAISAQFGVRDNNEASWT"
BASE COUNT         2481 a         3990 c         4086 g         2591 t
ORIGIN      
        1 gaacttcact gaacccaagg cggtgtggct cgctatcgat cagtagcatt aaaaaaagga
       61 tcgaacgcat gcggcgttcg atcctttctt gtttgcgctt gccgatatgc ggcgcagagc
      121 gatcttccga tcgctctttc cgaatctcaa tctccctgca acgccattcg ttcgcgcagt
      181 ttgatgagcg ccagcacgac gtcgatagtc ggcgtgggct cggcgacgag gcggcccatt
      241 tcctgcacca ccgtcaacag cggatcgatc tccatcggac gacggttctc caggtcgacc
      301 agggtcgagg tcttgtgtgc gccgaccgcg cccgcgccat caatacgctt ttccacgtcg
      361 acgcgaaagt gcacgccgaa ttgatccgcg atgcgcttgg cctcgagcat catggtgcgc
      421 gataccgcgc gcgtgccggg gtcgctggtg agcacgtcga gcgtggcatg cgtcaatgcg
      481 ctgatcgggt tgaagcacag attgccccac agcttgagcc agatttcgtc gcggatattg
      541 tcgcggatcg gcgcatcgaa acccgccgcc tgcatgattt cgtgtagctg ttgaatgcgc
      601 ggcgtacgtt ctccgctcgg ctcgccgatc gggaatttct tgccgtacac gtgcttgatg
      661 acgcccggtt cgacgatctc cgcggcggga tacagcacac agccgatcgc gcgttcgggg
      721 ccgagcttgt tccattggct gccgtccggg tcgatgctgg cgaggcgcgt gccggcgaat
      781 tttccgccgt gctggtaaaa gtaccagtag ggaatgccat tgacgccggt gacgatcgcc
      841 gtgtgcttgc cgaggagcgg ctgcatggcg tcgaccacgc cgggtaatga gtgcgccttg
      901 agcgtgacga tcacgacgtc ctgcacgccg agttcggctg ggtcggacgt gcagcgtacc
      961 ggcgcgctga acgtttcacc gtccatgatc aggcgcgcgc cgtgttcgcg catcgccgcg
     1021 agatgcgcgc cgcgcgcgat gaaactcacg tccgcgccgg cacgcgccag ttgcacgccc
     1081 attaaaccgc cgatcgctcc tgctccgaag atacagatct tcatgatgct gcccctgtga
     1141 cgatgagaag tagcttgccc gatgccgcat attagccttg ctgcggtggc gggtcactct
     1201 ttgagcatac gggcgcagga cgattactac tagttaaagt ttatgcgttt atgcattagc
     1261 tatcgtttat atatgcgcag acgggcgcgg ttaccgtgcc ccggtttaaa tggtcagtga
     1321 aaaacctgtg tggccgagcg tcagcggcga gatggttcgg taagcttagt attgcgtttc
     1381 cggcgacgac gactacgtcc gccgaaccag cagaaagctc gcgccgatta ccgccaggaa
     1441 cgccccgcag cagcggttga accagcggcg aacccgcgtg cgaaccagcc acttgccaag
     1501 atgcatgcct gcccacgaat aaagcgcgat cgcgacccat tcaagtacca gaaagctcgc
     1561 gccgagcacg gcgaactgcg gcagcatcgg tttggcgata tcgacgaact gcggcagaaa
     1621 tgcggtgaaa accagaatcg ccttcggatt gcccgccgcc acgaagcatt cttgccgcgc
     1681 gatccgccag agcgacgcat cgtcctgccg ggtcgtatcg atggccggcg cgtcgctgcg
     1741 ccacaactgg atcgcaagcc agatcagata ggcggcgccg gccagtttga tcgccagaaa
     1801 gaaccactcg gatgcgtgca gcacgacggc aagtccggtc gcggccagca ccagcatcaa
     1861 cgcgaacgcg acaagccggc cggtgccgcc gacgaacgcc gtcataaagc cgtgacgcgc
     1921 ggcgacgttg atcgacagaa gattgttcgg gccgggcgcg aggttgatcg cgaagcaggc
     1981 gggcagaaag aacagccagg cggtgatgga catgacggct cgcacggaga aggggacgtg
     2041 agtgcaacca attgggcgcg cgaaatggcg aagcggcaag caccggcgac gtcctgattt
     2101 cgcgtttccc cgatatgtgc caattgtagc gaatcggcga acgcaccgat tcagtccgtc
     2161 ggctcaattc accgatttga cgaagcctgg gtcgctgatc aacgcatcca cgcttagctt
     2221 gaccgtgtcc tcgacggcga tgatgttgct ggtgaattta ggcgacttct ggcggaccgt
     2281 cttgatggtt gagaacacaa ctttctgggt cgtggcgtcg gtcacgacgt agcgcatttt
     2341 cagtgtccag aatgctggag agcgctcatc gtctaccgtg aacttctcga tgctgccgct
     2401 cagaaccttc cggccctcgg tgagatgaaa gccggcgacc tgcaagctgc tggcaatgct
     2461 gttgcgcacg gtttcgttga tatcgttttt caacacgatg ccggtcatcg ccgtgttttc
     2521 gatccggttc ggcgacacct ggccgttgcg agccggcatg taggcgaaat cgcgggcggg
     2581 ggcaacgctg agccagccgg ttgaatgcgt ggccgcgcgc ggtgggctat tcaggccgaa
     2641 ccaggaccag gagcaggcgg acagcatcaa cggcagtgcg acgacgccgc aacccttgag
     2701 cacagcggat ctcagggccg gacgcggggg accgagcgat tgccgcagag actggctctg
     2761 ttgcggcgac tcggacgctg tgctggaaaa agcggcagtc gaggtcagag gcgttgcctt
     2821 ttaagttgga tcatcccggt acagagcagc atggtaacag cgagccgatc ccgtggatcc
     2881 tcttaactgg atcaggggtc ggaatgctcg aacgatcgaa acagccacgc tcatcataac
     2941 gatcaaaaaa atccggcttt cccctgagtc atatccacga gggctaaccg ggcatcgtcc
     3001 accgcgttga cgggcagccg gacagtcatc ctgaccagca tgccgtagac gctgtccacg
     3061 agtccgtgac cttcggcctc gatccatctg cgcaccttcg cttcatcggc gtagctcact
     3121 tcgacgatca gcgcaacctg ggcaatcctt tccacccgcg gtgcgtccag gagcgccgca
     3181 gcaatcgcgt cggtatacgc gcgcaccagg ccgccggcgc ccagcttcac gccgccgtaa
     3241 taccgcacga cggccgccag cacgccgtcc agatcatgat gccgcagcac ttcgagaatg
     3301 ggccggcccg cggtgccgga cggctcgccg tcgtcggaca tgcctgactg gccgcctgcc
     3361 agcagcgccc agcacacgtg cgtcgcgcca ggatgttcgt cgcgcaggcg ccgcaactcg
     3421 accatcgcgg cgtcgcggtc ggcgacggga atcgcatgcg cgatgaaacg gctcttacga
     3481 atctccagtt cggcggagag cggcgcgggc aaggtataag tgggcaaggc aaagacttca
     3541 gtggatgaaa aaatgcgggc ctcatgcatg gccacatgcg gatctcgtgc gtgggtctca
     3601 cgcggccctg tgcccgcaat ggtaacggat cgccaatagg taatcggcgt tcgtctacaa
     3661 tcgtccgctg aacgcgcgcc ttccggctgc accgggcgca ccaccaagga gacaacatgc
     3721 ttgatttttc cacgctagga acgttcgttg ccgttgtatt agggctcttt ctgattcccg
     3781 gtccggccgt gctgctggtt ttgacgcgta cggtgcatgg tgggcgcaag gcgggcatac
     3841 tcaccggtct cggtatcgcg gtgggcgatt tcatccacac cctgggtgcg gcggtcgggc
     3901 tgtcagccct gctgatgaca tcggcgctag ctttcaatgc cgtgaaattc gtcggcgccg
     3961 cctatctcgt ctatctgggt gttcgcgcct tgcgagagaa gcaggcgagc gcgcatttgc
     4021 cggaggtggc gccggtatcc gcttctaaag ccttctttca ggcgatccct gctgaagtgt
     4081 tgaaccccaa gaccgcgctg tttttcctcg cgttcctgcc gcagttcgtt cgtcctgagc
     4141 acggctccac gttcctgcaa ttcacgacgc tgggactcat cttcgtcggc atgagcgcgc
     4201 tttatacgac gctgatcgtg ctgaccattc gtccgctggg caaactcgtc aaacgcctga
     4261 cctggcttag ccgctggcaa aacaagatca tcggcgtgct gttcatttcg ctcgggttgc
     4321 gcgtcgctac gcaaacgcgc tgacgagtcc ggaagccgca tgcctttttt gggttcgggc
     4381 gcgcggaaat cgcgttgcca cgctcgcgct agatattccg gcgctgccgt ctgaggcggc
     4441 cttcgcgcgc gtattccatc tggccctcgg tgcgcacggc gagccgttcc tgttcggccc
     4501 ggctcatctg ccgcgtatcg tcgatgtcgc cgacccgcag cacttcctgc gcgatccgta
     4561 tgcgtaatgt cattaagggt tcaccgcgtt cgaccagcgt gcggtcggcg gcgcgcgctt
     4621 gctggacgcc gctgtcgatc aggttgcgca actccagcag cgtgagccgc tggcgctgga
     4681 tctcgagaat gagccgctgg atggcctgct cgtcgcgcgt gcgccaccag gcgcgcagtt
     4741 cggcaagcgt gggaggttcg aagggaggaa gacgcattgc tcagacgaaa agtactgtat
     4801 atgtgtacag tatatcccgt ctttttgggg caacgcatgg cgcgcgttgc ttgcctcgca
     4861 agccccgcaa acgggagcta caacgctcac gcgagcgtga gcatgatgga catcccttcc
     4921 gcctggctag cgtccacgtc ctttcgctgt ggctttgccc gttgccggtc caggtgacgc
     4981 gcccgcactg ccgcatatca atgccacccc ggcgaccgct gtcgcccgca acgacgcacg
     5041 cgaatcgccc gcacgggcgc gcaacgcgag gctatgcaaa atcgcggacg cgatcctcgc
     5101 gaggagaggc ggttgcgcat cggccgccag ttcgccagtt gcctgcgcca gcttgaagcg
     5161 tttctcgaag gccttatcgt aggtgcgcag tccctcggcc aacgtcgcac ggacctcggc
     5221 gtcatccacc gcttcgacca cggcggtgcc gatcatgaag cagccgagcg gatcgccgtc
     5281 ggcgggcaag taccaggcga gtgcgccgtc ataaacgcgc agcagtgcct gcggcaacgg
     5341 caggtcctca ctgagcgcat gaaccatcgc gagccggccc gcttcgatat aacgttcgag
     5401 ggtggccaga tacaacgcat gtttgtcgcc gaatgcgccg tacaggctcg ggcggttcat
     5461 gccggtcgcg gcactcaact gatccagtga ggtgcccgca tagccgctac gccagaacac
     5521 gtctcttgca tcggcgagcg cctggtcgga atcataggca cgcgggcggc cacgcgggcg
     5581 ggtttctttt tttgtactgg tcggcataaa aatatggcgt aagagattta tatgcaatat
     5641 agtacaaaaa caccaacgac aggggattcc tcatggatct gtacttctcg ccgctcgcgt
     5701 gctcgctggc cacccgaatc gcgttgtacg aggcgggagc gcaggctggg ttcatccagg
     5761 tcgacacgaa gtcgaaacgg ttgtcggacg gctcggactt ctatccaatc agtccgctcg
     5821 ggcaggtgcc ggtgctgcgt acggacgaag gctggctgtt gagcgaaaac accgcgatcc
     5881 tgccttatgt cgccgatcgt tatccggccg cacggctcgc gcctgccgcg ggcacggcgc
     5941 agcgcgcgcg gctgcaacaa tggttgggtt ttatcagcac ggagttgcac aaggcggtgt
     6001 tcgtgccgct actcgatgcg cgtgcgcctg aagaagtgaa gcgctatgtg cgcgacaagg
     6061 cctcgttgcg gctgggcgtt ctgcaagatc atctggcgca cagcgaatat cttctcgatg
     6121 ggttcagcgt gggcgacgct tatctggtca ccgtgctgaa ctgggcgtcg tatggcggca
     6181 tcgatctgtc gcaatggccg acagttgctg agtactacaa gcgattgatg cggcgcccga
     6241 gcgtcgccaa agccgcagcg gaagaaatcg ctttgtttga ggaagaagtc gcgaggcgtg
     6301 cgaaaagttg aggacaggtt gccggctgcg cgattagtcg tcgccgtgca ggcagaagcg
     6361 gcgccccgga atgtgcgaga cgaccgcctg agcgatttcg agtggcgtgc tttccgcagg
     6421 caccacgcgg cggctcgatt cgggggtgtg cttcatggtc cattcgacga gtctgtacga
     6481 gcgtccccac ggaggttgca aagggtctga gaccttgcac gagtggatct ggcgtaccca
     6541 ttcgttgccg ggcatcgcgc gcaaatgctc aaataccgtg tcttccacca ttgtttgact
     6601 ccttcttcgc aggcatgctg tccagctatc ggcgcgtcgg cgacatccct cgagaaaaaa
     6661 gcgccgccga tgttctggcg gctcaacagg ggatgttgtg cgtcatagtt acaggtaaga
     6721 attaagccaa acttaagatc tcgctcaaat ccgcaccggc tgacgcggcc gcgcaaagcc
     6781 gcacgatgat ggaagaggcc gatcgggcga tgtaccaggc caaacacggt ggccgcaatc
     6841 gcatcgaggc attggcgaaa gcgggcagcg ataatgcggc atgctgaccg tcgcgaccag
     6901 ccttttattt cacgcatccg gcggcacgcc cagcaattgc aatgcaccgc cggtgacccc
     6961 gctaaacact gggccggcca ccgtcccacc ataaaatgcc ttgccggcgg ggtcgtcgat
     7021 catcacggcg acgatcagcc gcgggtcgct catcggcgcc atgccgacaa acagcgcgcg
     7081 gtaacggttt ttcgcgtaag tcgcgccgac ctgtttgcgt gccgtgccgg ttttgccgcc
     7141 gatccggtag ccttccaccg ctgcggcgcg ccccgtgccg ccaggccccg cagccatttc
     7201 gagcatcgag cggatcgcgc gcgcggtggc gggtgtcgtg actgcgtgcc ccttgtcgcc
     7261 ggcctgcgtc gcggtgccaa cgtccgcgtt tctgtccgcg actttggccg cgcttgtgtc
     7321 cgagttactg tccgcgtttg tgtctttacg cagcagactc acgtgatgca gggtgccgtc
     7381 cccggcatag gcggtgtaga cctgcgcgat ctgcagcaac gaagtggaca ggccatagcc
     7441 atacgccatg gtcgcctgtt cgatcgggcg ccagcgtttg tatgggcgca ccttgcccga
     7501 tgcgacaccc gggaaggtca gctcgggcct caacccaagt ccatattcct gatacttgtt
     7561 ccagatcttt tcggcgggca ggttcagtgc gagtttggca agtgcgatat tgctcgactt
     7621 ctgcacggct tcggcgacgg tcatcgcgcc atgattcgac gtgtcgtgaa tcacggccgg
     7681 gccgatcttg taccagcccg gcgcggtatc gatgacgctt tgcggccgta ccttgccttc
     7741 gtcgatcgag agggcgacga cgactggctt gatggtcgag cccggctcga aggtatcgac
     7801 caccgcgcga ttgcgcagtt gccgcccggt gagacgggcg cggtcgtttg gatcgaagct
     7861 cggatagttg gcgagcgcga gaatctcgcc gttgcgcgca tcgagcacga ccacgctgcc
     7921 ggcttcggcg tgatgtttgg cgacggcgtc cttcagttgt gcgtaggcga gctgctgaat
     7981 gcgccggtcg atcgtcaggc ggatcgtgtc gccgttttgc gcgggcacga gcggacgggt
     8041 ctcggacacc acccggccca gccggtcgcg gatcacttcg cgctggcccg gcacgccgag
     8101 cagctgctcg ttggcggcga gctcaacgcc ttcctgaccg ttgtcctcga tatccgtgaa
     8161 gccgaccacg tgtgccgccg attcaccctc ggggtaaaag cgcttcgaat cggcgatctg
     8221 cgtgatgccg gcgaggccca gtttgttgag gtggtcggcg gtgtcggcat tcacctggcg
     8281 cttgagcagc acgaaggtct tgtcgccgtt cagacgccgg cgcagttccg ccagcggcag
     8341 atcgagcagt ctggaaagcg gcgcgaacgc ggcttcgtcg agcagcctgg gcgtggccca
     8401 gatctcatag gtcgcaaggc tgacggcgag catcgaacca ttgcgatcga cgatgtgccc
     8461 gcgcgtcgca tcgagttcga tggtgcgctg atagcgtctt tgcccctgat cgacgtagaa
     8521 gtcctgattg accacctgca cccagaacgc gcgccccgcc agtgacgcga acgcgccgaa
     8581 caccagcagg acgacaagtt tcgagcgcca catcggcagg cgcgcggcca gcagcgggtt
     8641 cttcgcgacg ggcgcgtacg gatcgtgcga ctgcgacttc ttcttctgga tcatgagtgc
     8701 ggtgagcgaa cagcgatgcg tggcgagctg gaaccggcga tgccggcggc tggttgcgcg
     8761 tggctgtcga gcgtgctcct cgagtgaagg ccacacgcgc gtggcgtgca gcgggattag
     8821 cttgcgccgc ccgaatcggc ggcggtgaat accttgagtt gccaacgaaa ccgcacggcc
     8881 agcatgcgca ccgcaaagcc gccggccaat accacgatgg aggccggccc cgactccacg
     8941 ccccaggctt gcagcgcgac atacagcgcg cccgcgccga gcgcgatgct cgcgtacagc
     9001 tcctcacgca gcacgagcgg catctgattg cacagcaggt cgcgcagcat gccgccgcac
     9061 acgccggtga ttgcgccggc cagcacgaca atcgtcggac tcacgtcgag cgtcgcggcg
     9121 acatcgcaac cgatgaccgt gaacgcgatc aggccgagtg cgtcgaccgt gacgaacagc
     9181 cgttgccagt tgcgcaacca tttcgcgctg gccgcggcaa tcgacgccgc cccgattgta
     9241 atcagaacgt aatctggatg cgagatccat gcaaggggat agtggcccag caacacgtcg
     9301 cgcaccgtgc cgccgccgag cgcggtgacg gtgccgacga ggcacagacc gaagcgatcc
     9361 atgccgcggc gcatgcccat caatgcgccg gacatggctt cggcgacgat cgcaagcagg
     9421 tagatgaggt ggagcaacat gacagcgggc cctggatggc ggcgctcgcg aaaggaaggg
     9481 ctctgcctgg cgcttgccgc gaggcgcgcg tttgaaagcg gccattatcg gaagcggcgc
     9541 gtgctgatga aaatgcctta agccgaattc ggcgatgcca aattcggctt aatctggttg
     9601 cgggattggg cgtggtaaac gttcgctgaa tggtgagggc ctttgtcctg gcgcggcaga
     9661 cgcagacata ggcacagacg caggcgcagg cgcagacgca ggcacaggca caggcacagg
     9721 cacaggcaca ggcacttgcg cagatgcgga caccggccac aaaaaagccg cgcggacttg
     9781 aaaaatccgc gcggctgttt cgttcgatta atgcgtcgac tccgacgcgg cgtgttaaag
     9841 aggcgctctc tgcctgtaga gcgccttctt tttaccgcct tcttttcccg gtgtcccgct
     9901 gggcagcggt gagtctggtg tcgtgcctgc tgattggctt ctacttcact cgtccgacgg
     9961 cgatccacgc gaacatgaaa gcgttcaacg tgaaccgcat cggcgacttc aggtttctgc
    10021 ccagcagcag ctagcggcct caggccggca aagcgaagct cgagatcgcc tcgcgcagca
    10081 cgcccacctg gtccttcagc gaatgggccg cggcggcggc ttgttcgacg agcgcggcat
    10141 tctgctgcgt gacctgatcc atttcgccga ctgcgcgatt gacctgttcg atgcccgcgc
    10201 tctgttcgcg cgacgcgtgg ctgatctcct cgagaatttc gttgacgcgg cgtaccgact
    10261 gcacgatctc gcccatggtc gaaccggcat tggcgaccag cgacgcgccg gcgtcgacgg
    10321 aatgggtcga ggtctcgatc aacgccttga tttccttcgc ggccgtggcc gagcgttgcg
    10381 cgaggctgcg cacttccgcg gcgaccaccg cgaagccgcg tccctgttcg cccgcgcgcg
    10441 ccgcttcgac ggcggcgttg agcgcgagga tgttggtctg gaacgcgatg ccgtcgatca
    10501 cgccgatgat gtcgccgatc tgccgcgagc tgctggtgat ttcgcccatt gtgcggacca
    10561 cgtcgtcgac gacgcggctg ccacgggtgg ccacatccgc ggcctgaccg gcgagttgtg
    10621 ccgcttgcag cgcgctgtcc gcattctgct tcacgttgac cgtcatctga tccatgctcg
    10681 acgccgtttc gacgagcgcc gcggcctgtt cttcggtgcg ctgcgagaga tctgtgttgc
    10741 cggcggcgat ttcggttgca ccgatattga tgttttcggt accgaggcgc acgcgcgaaa
    10801 ccgtatcgac cagaccggcc tgcatggtgt ggagcgcgtg cagcagactg ccggtgtcgt
    10861 ttgcttgcac cggcacgcgc gtggcgaggt tgccgcgcgc catcaattgg gccgcttcga
    10921 ccgcgacttc gagatcgccg ccgagcgtgc gacggatgct gcgcaacacc agcagcatga
    10981 cgatggtggc gattgcgccg agcgtcacgg tgatggcgag ccagcgcagc agattggcgt
    11041 agaacgcgcg ctgcacgtcg tccatgtaca tgccggtgac gatgtaccag tcccacggcg
    11101 cgaagtggac cgcgaagctg gtttttgcga cgggtttgtc gctgcctggt ttggaccaca
    11161 gatagtcgac gaagccgccg ccgtcgtgat tgcctgcgtt gacgatgtcg acgaacagat
    11221 ggttgccggc cgggtcggtg tactgtgcca ggtttttgcc gaccatgtcc tgcttgattg
    11281 ggtgcatcag catgacgggc tgcgagtcgt tgacggagat gtagccgtct ttgccgtagc
    11341 gcatggcggc gagcgattcg agcgcctgct ttttggcgtc gtcttcggtg atcgtgtgtt
    11401 gttgcgctag cgtgtagtag cggttgacga tgtggttggc ctggtcgatc agcgaggtga
    11461 gctgatcgcg gcggtccgag atcatcgacg cgcggttttg ccatgcgccg aagccgccga
    11521 tcaggatcag gccggtccag aggaccgcga tcattgaggc tagttttttg ttcagggtca
    11581 tagcagtggg atttgctgtt tacttcgctt tcgcgagggg gcgggcaggg tgttgcgctt
    11641 tactctttca ctcttttgct ttttcgcttg cagcggtggc ttgctgctta ctgtgtttac
    11701 ggcgatgttt ctttgctctg caggggaggg gaaacccgtt gtttatttgt ctgattaaat
    11761 gaagattttt ttttccctgc ccggggctta ttcggccgtc ggcatgcggt ttggcctttc
    11821 cttggtctgg ttttttggcc tttccttgaa ttgatattgg tttattagcg tcgcccctgt
    11881 gcggggcggg cacctacttt tctttgcctg ccgcaaagaa aagtaggcaa aagaaagcgg
    11941 ctcacaccgc cagcgcataa gcgggtcccc cgcacagtca cggtagcggt gcatctggaa
    12001 tccgtgttct cgcatactcc gcgtcagtga cacagcagtc atacttccgg cggcgctgcg
    12061 cgccccgaag ggtgcttcac aaaaccaatg gttttggagt ggatcgccac agagcacttc
    12121 acaaaaccaa tggttttggg cggatcgtcg cagagcactt tcaaaatccg tgagtgttgg
    12181 gcacgtcggc aaagggcggt tcataaaacc gttgggtccc aggcgcctcg gcgaggctaa
    12241 gccgatagtg cgcacagccc gtaaacaagg cccgtggttt cctatgtagc gccattttcg
    12301 ccgaggcgaa gccgacggcc ccatcacgcc caaacgaaac cattggtttc cgtgatgaac
    12361 ctttccggcg aacacgcagt gtgaggcggg aagtatgact gcctggtcac cgacgctaag
    12421 tttgcgaggg cacggattcc agatgcacca ctaccctaac tgtgcgaggg acccgcttaa
    12481 gaatttgcgg tgtgagccgc tttctttgct tatctttctt tgcggcggca aagaaagtaa
    12541 gtgccgcccc gcacaggggc aacactaata gaccactaac ataattaatg cagaacgcac
    12601 ctaaaccaat aaaatacaaa aacccaaaat aaaccaaatc caataagaaa cctaaaaata
    12661 ccctaaaata aacataaaaa gaaaaatccc atcaactcca tgcccagcag ggcccaaatc
    12721 acattcctcc acacaacgcc aaaaaacacc aaaaaacaga aagccctgca tgaaatgcaa
    12781 aaaacaacac atttccccac ttaaaaacaa catcagaaaa cccaaacaaa ccccaacaac
    12841 caatagaaac attttccgtt atagtcgcca ttttccagcc gcccgccggg caaaaaaaga
    12901 gccctaataa gcccgccatc aggagtaaag aaaatgaacg cccgcgaacc cgcattcatc
    12961 tccgcttcgc gcagcgcacg cctgcgccag atgctcgtca gcaacgaact cgaattcatg
    13021 atggaggccc acaacggcct ctccgcccgt atcgtccgcg aagccggctt caaggcgatc
    13081 tgggcttcgg gcctcgcgat ctccgcccaa ttcggcgtgc gcgacaacaa cgaagccagc
    13141 tggacgca
//
LOCUS       DATASH010000944         4291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3719687,
            whole genome shotgun sequence.
ACCESSION   DATASH010000944 DATASH010000000
VERSION     DATASH010000944.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3719687"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VHV99_25245"
     CDS             complement(<1..350)
                     /locus_tag="VHV99_25245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemoreceptor glutamine deamidase CheD"
                     /protein_id="HEX3638726.1"
                     /translation="MSSSLPIATNLYFDNHFQRPGVKLLPNEFYTTHEDMVLVTVLGS
                     CVAACIQDRTAGIGGMNHFMLPDDGADAGQPASDSMRYGAYAMEVLINELIKAGGRRE
                     RFEAKVFGGGAVLAG"
     gene            complement(347..1264)
                     /locus_tag="VHV99_25250"
     CDS             complement(347..1264)
                     /locus_tag="VHV99_25250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008924153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEX3638727.1"
                     /translation="MEPARPGEQGRDFEFTSADFARIRDLIHRGAGISLSDHKRDMAY
                     SRLARRLRARGLETFKQYLDLLEAENDPAEWEAFTNALTTNLTAFFREAHHFPILAEF
                     VPRRAQPVSVWCSAASTGEEPYSIAMTLIEALGDSGARQASVLATDIDTQVLAKAEAG
                     MYQFDQVKHLSPERLKRFFLKGTGAHTGMVKVRPEVRAMVRFEQLNLTDRDYQLRAQF
                     DAIFCRNVMIYFDKPTQAQVLARFEPLMKSGGLLFAGHSENFTYVTQAFKLRGQTVYE
                     LTRDAGAAAGTPARATGHAGSAKTSGVIA"
     gene            complement(1550..3499)
                     /locus_tag="VHV99_25255"
     CDS             complement(1550..3499)
                     /locus_tag="VHV99_25255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX3638728.1"
                     /translation="MLSRWSIRTSLTIVGIILVALTVVVGALGLTALNDSSQSLDRIA
                     RGDLVAIHALDDASSYLLRSRLAVDRFNTLSAAGNADEAKKAIDRAQELLTKGNQSWQ
                     SYLDAPKKGIDQALLDDVVARRTGLMHDGLDPEFAALNANDVAAYHAIADTKISPMFI
                     AYDNAATAVVKALQQSTMDQQAGAQSNISLMTTLIVIVTAIALLLVVGIRFALGGLIV
                     QPLNDATACFERIASGDLSERIEVFSRNEVGRLFAGIKRMQDSMATMVKSVHSSAESI
                     DTGAREIAMGNTDLSQRTEQQAASLQETASSMEQLTGTVRQNAENARQASQLAVNASD
                     IATRGGDVVSQVVTTMEGIAASSRKVVDIIGVIEGIAFQTNILALNAAVEAARAGEQG
                     RGFAVVAGEVRSLAQRSASAAKEIKELIGDSVDKVQSGSALVGRAGTTMDEIVQAVRR
                     VTDIMGEISAASEEQSGGIEQVNRAVVQMDAVTQQNAALVEQAAAAAASLEEQTRQLQ
                     AVVNVWKVADGQTRAGATAVRTQAAMRRNGGTNGSANSNADGATLGGKYAQPARAPQQ
                     AHGNAAAHSAASSATSAATHTATHTATHTATHAAPASANHGADTARAEPALKPRTARA
                     ASESTARPAVASAASAGSDADWETF"
     gene            complement(3591..4121)
                     /gene="cheW"
                     /locus_tag="VHV99_25260"
     CDS             complement(3591..4121)
                     /gene="cheW"
                     /locus_tag="VHV99_25260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX3638729.1"
                     /translation="MAEVQSINSNVANATNGRRDARQADAGGQEFLVFTLGAEEYGID
                     ILKVQEIRGYDNVTRIANAPEFIKGVINLRGIIVPIVDMRIKFHLGSVEYDHQTVVII
                     LNVAHRVVGMVVDGVSDVLTLATDQIMPAPEFGATLTTEYLTGLGTVDGRMLILMDIE
                     KLMTSREMALIESLGA"
BASE COUNT          682 a         1414 c         1338 g          857 t
ORIGIN      
        1 ccggccagca ccgcgccgcc gccgaacacc ttggcttcga agcgttcgcg ccggccgccg
       61 gccttgatca gttcgttgat cagcacttcc atcgcgtacg cgccgtagcg catcgaatcc
      121 gacgcgggct ggccggcgtc cgcgccgtcg tcaggcagca tgaaatggtt catgccgcca
      181 atgcccgccg tgcgatcctg gatgcaggcc gcgacgcacg aaccgagcac ggtgacgagc
      241 accatgtctt cgtgggtcgt gtaaaactcg ttcggcaaca gcttcacgcc gggccgttgg
      301 aagtgattgt cgaagtacag attcgtggca atcggcaggc tgctgctcat gcgatcaccc
      361 cgctagtctt tgcgctgcct gcatggcccg tggcgcgtgc cggcgtgccg gcggcggcgc
      421 ctgcgtcgcg cgtcagttca tagacggtct ggccgcgcag cttgaacgcc tgcgtcacgt
      481 aggtgaagtt ctccgaatgg ccggcgaaca gcaaaccgcc cgatttcatc aacggctcga
      541 agcgcgcgag cacctgcgcc tgcgtcggct tgtcgaaata gatcatcacg ttgcggcaga
      601 agatcgcgtc gaattgcgcg cgcaactggt aatcgcggtc agtcaggttc agttgctcga
      661 agcgcaccat tgcgcgcact tcggggcgca ccttcaccat gccggtatgc gcgcctgtcc
      721 ccttcaggaa gaagcgcttg agccgctcgg gcgacagatg cttcacctgg tcgaactggt
      781 acatgccggc ttcggctttc gccagcactt gcgtgtcgat gtcggtggcg agcacgctcg
      841 cctgacgcgc gccgctgtcg cccagcgctt cgatcagcgt catcgcgatc gagtacggtt
      901 cctcaccggt cgaggccgcc gagcaccaca ccgaaaccgg ctgcgcacgg cgcggcacga
      961 actcggcgag gatcggaaaa tgatgggctt cgcggaagaa cgcggtcagg ttggtggtca
     1021 acgcattggt gaacgcctcc cactcggccg gatcgttttc cgcttcgagc agatcgagat
     1081 actgcttgaa ggtctcgagg ccacgggcgc gcaggcgccg cgccagacgg ctgtacgcca
     1141 tgtcgcgctt gtgatccgac agcgaaatac ccgcgccgcg atgaatcaga tcgcgaatgc
     1201 gggcgaaatc cgccgacgtg aattcgaaat cccgtccttg ctcgccgggt ctggccggtt
     1261 ccacccgctc cggaactgcc ggttgcggag gtgccgcttg cggaggtgcc gcttgccggc
     1321 gttgctgtgc gcgcgttgcc atcatcagag ttcctgcctg cgatacgagc catcccactt
     1381 ttcgcccgaa gcatggcttc cggcgaaagg ggttgtgttc gcggggtcgc gcccgcccgc
     1441 gagttctgcc aacacgcagt cggcccgcga cacgcttctt aagcgtgctt cgatgattcg
     1501 cccgtcatgg agcgagatgc cgaatgaccg catgtctttg tgccacttcc tagaacgttt
     1561 cccagtccgc gtccgatcct gccgacgccg cgcttgcaac ggccggacgt gctgtggatt
     1621 cggatgcggc gcgggccgtc ctgggtttca gagccggttc ggcacgtgcc gtgtcggcgc
     1681 catggttggc cgaagccggg gctgcgtggg ttgccgtgtg ggtagccgtg tgggttgccg
     1741 tgtgggtggc cgcggaggtt gccgaacttg cagccgaatg cgcggcggcg ttgccgtgcg
     1801 cctgctgcgg cgctctggcc ggctgagcat acttgccgcc gagcgtcgcg ccatccgcgt
     1861 tgctgtttgc gcttccattc gtgccgccgt tcctgcgcat cgcggcttgc gtacgcaccg
     1921 cagtggcgcc ggcgcgcgtc tggccatcgg ccaccttcca gacattcacc accgcctgca
     1981 attgacgcgt ctgttcttcg agcgacgccg ccgcagccgc cgcctgttcg accagcgcgg
     2041 cgttctgctg cgtcaccgcg tccatctgca cgaccgcgcg attcacctgt tcgatgccgc
     2101 ccgactgctc ttcggaagcc gcgctgattt cgcccatgat gtccgtcacg cggcgcaccg
     2161 cctgcacgat ctcgtccatc gtggtgcccg cgcggccgac cagcgccgag ccgctttgca
     2221 ccttgtcgac cgagtcgccg atcagttcct tgatctcttt cgcggcgctc gcgctacgct
     2281 gcgcgaggct acgcacttcg cccgcgacca ccgcgaaacc gcggccctgt tcgcctgccc
     2341 gtgcggcttc gaccgccgca ttcagcgcga gaatattggt ctggaaggca atgccctcga
     2401 tcacgccgat gatgtcgacg accttcctgg aactggccgc gatgccctcc atcgtcgtga
     2461 cgacctggct taccacgtcg ccgccgcgcg ttgcgatatc cgacgcattg acggccagtt
     2521 ggctcgcctg gcgtgcattc tcggcgttct ggcgcacggt gccggtcaac tgctccatgc
     2581 tcgaagcggt ttcctgcagc gatgcggcct gttgctcggt gcgttgcgag agatcggtgt
     2641 tgcccatggc gatttcgcgc gcgccggtat cgatcgattc ggcgctgctg tgaaccgact
     2701 tcaccatcgt cgccatgctg tcctgcatcc gcttgatgcc ggcgaagagg cggccgactt
     2761 cgttgcggct gaatacttcg atccgctcgg acagatcgcc cgacgcaatc cgttcgaagc
     2821 acgccgtcgc atcgtttagc ggctgcacga tcaggccgcc cagcgcgaag cggataccca
     2881 cgaccagcag caacgcgatc gcggtgacga tgacgatcaa cgtggtcatc agcgagatgt
     2941 tcgattgcgc gcccgcctgc tgatccatgg tgctttgctg caatgccttg accaccgccg
     3001 ttgcggcatt gtcgtaggcg atgaacatcg ggctgatctt cgtgtcggcg atggcgtgat
     3061 aggcggcgac gtcgttcgca ttcagggccg cgaactccgg atcgaggccg tcatgcatca
     3121 agcccgtgcg cctcgcgacc acgtcgtcga gaagcgcctg atcgatgcct ttcttcggcg
     3181 catcgagata gctctgccag ctctgattgc ctttggtcag caactcctgc gcgcgatcga
     3241 tcgctttctt cgcttcgtcc gcgttgccgg cggccgagag cgtgttgaag cgatccaccg
     3301 cgagacgcga acgcaacagg tacgacgacg catcgtcgag cgcgtgaatg gcgaccagat
     3361 cgccgcgcgc aatacggtcg agcgactgac tcgagtcgtt cagcgcggtt agaccaagcg
     3421 cgccgaccac gacggtcagc gcaaccagaa tgatccccac tatcgtcagc gatgtgcgaa
     3481 tcgaccacct gctcagcatc ttggcactcc ctgttcgatc tatctttcgt actcacgcga
     3541 actgacgcgt tttgacgttc tagcggcccg gcggtccggc gatccggcgt tcaggcgccg
     3601 agcgattcga tcagcgccat ttcgcggctg gtcatcagct tttcgatgtc catcaggatc
     3661 agcatgcggc cgtcgaccgt gcccagccct gtcaggtact cggtcgtcag cgtcgcgccg
     3721 aattccggcg ccggcatgat ctggtcggtg gcgagcgtga gcacgtccga cacgccgtcc
     3781 accaccatcc cgaccacgcg atgcgcgacg ttcaggatga tcacgacggt ctgatggtcg
     3841 tactcgacgc tgcccagatg aaacttgatg cgcatgtcca cgatcggcac gatgatgccg
     3901 cgcagattga tcacgccctt gatgaactcc ggcgcattgg cgatacgcgt gacgttgtcg
     3961 tagccgcgga tttcctgcac cttgagaatg tcgatgccgt actcttcggc gccgagcgtg
     4021 aagacgagga attcctgacc gccggcgtct gcctgccgcg cgtcgcggcg gccattcgta
     4081 gcgttcgcga cgttcgaatt gatggattgg acttctgcca cgttagcccc caaacggttg
     4141 ggatgaaata aattgagtga gtgttgcgac aactgcgtgc gttgcaaacg ccattgggaa
     4201 tgccatgacg gatctcatgc gaggctcagc gcgccgtgcg catggcgcgt ttcgcgattc
     4261 agcgccgcca cgtccacgat cagcgcgacg c
//
LOCUS       DATASH010000945         4934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_953277,
            whole genome shotgun sequence.
ACCESSION   DATASH010000945 DATASH010000000
VERSION     DATASH010000945.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4934
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_953277"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1070
                     /locus_tag="VHV99_25265"
     CDS             <1..1070
                     /locus_tag="VHV99_25265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEX3638730.1"
                     /translation="DYHLIPFAQALRAAGVKNRIGFFLHIPFPASQVLLAVPPHRELV
                     EALCSFDLLGFQTAPDLRAFCDYVVNEANGTVDVAASGPLTVHAFGRSLRAAAYPIGV
                     YPDEIAELAKAGERGKPVRTMKTALHSRKLIMSVDRLDYSKGLVERFRAFERLLEHAT
                     AQRNKVSFLQIAPPTRADMHAYQDIRLQLEGESGRINGRFAELDWTPILYIHKQYERS
                     VLAALFRTAHVGYVTPLRDGMNLVAKEYVSAQDPENPGVLVLSRFAGAAQELDGALIV
                     NPVDIDGMAEALAKALEMPLVERQARHRDMMVQLRENNVSVWRDNFMRDLQSVDAAKS
                     TRTGKVRAGSARKAVRETLAG"
     gene            complement(1204..3039)
                     /locus_tag="VHV99_25270"
     CDS             complement(1204..3039)
                     /locus_tag="VHV99_25270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3638731.1"
                     /translation="MEHLTIAQRNALNAKLASYAHRPLAFLFRFIRRHPLAHAIVLCS
                     VFAAVGCALASQYAIKHLIDVLGEGRHHPGPLWGAFAILVGLIAADNLLWRVGGWVAA
                     HTFVAVTGDLRRDLFQYLSGHSPTYYSEKQPGMLASRITATSNAVYTAENTTAWNVLP
                     PCIAVLGAIIMIIAVNPLMAGGLMLCSAILSVVLYKLAGRGSARHHHFATKAASVDGE
                     LVDVISNMGLVRAFGMTFREQQRFGATVKAEMEARQQSLLYLEKLRLLHAVITALLSA
                     GLLGWALWLWDQGKATSGDIVLVSSLGFTILHGTRDLAVALVDVTQHVARLAEAVRTL
                     LEPHGMPDRDGASELVPQGGRVDFESVTFAYPRRRPILDHFDLHIEPGQRVGLIGKSG
                     AGKSTVLALLQRFYETQGGAIRIDGQEIAGITQDSLRHAIALVPQDISLFHRTVYENI
                     AYGRPEASREEVVAAAREARCSDFIEAMPEGFDTIVGDRGVKLSGGQRQRIAIARAIL
                     KNAPILLLDEATSALDSASEEAIQKALDRLMVGRTVIAIAHRLSTLNNFDRIIVMSQG
                     KVIDDGSPEALRQRPGLYRDLLSKQYGKGTTLHIGGKKVDEQHVV"
     gene            3731..4795
                     /locus_tag="VHV99_25275"
     CDS             3731..4795
                     /locus_tag="VHV99_25275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX3638732.1"
                     /translation="MRIAQIAPLHEAVPPKLYGGTERVVSYLTEALVEQGHDVTLFAS
                     GDSQTSAKLEAFWPQALRLDPTIRDVMAPHMLLLEEVRRRADEFDVLHFHIDYYPFSL
                     FARQPVPFLTTLHGRLDLPELQPIFNTFSDVPVVSISDNQRSPLQQANWLQTVYHGLP
                     ENVLTPIKDVEPGYLAFLGRVSPEKGLDRAIRIAGQAGMKLKVAAKIDKADRAYYEEV
                     IKPLMALPHVEYIGEIGEAEKREFLGNAHALVFPIDWPEPFGLVMIEAMACGTPVVAF
                     NRGSVPEVIENGVSGFVVEDEISAVAAVKRLHTLPRAKVRAAFEARFSSKVMAQNYVA
                     TYEALLRQKHRTVLREVNAS"
BASE COUNT          930 a         1501 c         1565 g          938 t
ORIGIN      
        1 acgactatca cctgattccg ttcgctcagg cgttgcgcgc ggccggcgtg aaaaatcgca
       61 tcggtttctt tctgcatatt ccgtttccgg cgtcgcaggt attgctggcg gtgccgccgc
      121 atcgcgagct ggtcgaagcg ctctgttcgt tcgatctgct cgggttccag accgcgccgg
      181 atttgcgggc gttctgcgac tacgtcgtca acgaggcgaa cggcacggtc gacgtggccg
      241 ccagcggacc gctgaccgtg catgcattcg gccgcagctt gcgcgcggcg gcttatccga
      301 tcggtgtcta tccggacgaa atcgccgagc tcgcgaaagc aggcgagcgc ggcaagccgg
      361 tgcgcaccat gaagacggca ctgcattcgc gcaagctgat catgagcgtg gaccgcctcg
      421 attattcgaa ggggctggtc gagcgttttc gcgcgttcga gcggctgctg gaacacgcga
      481 ccgcacaacg caacaaggtg tcgttcctgc aaatcgcgcc gccgacgcgc gccgacatgc
      541 acgcctacca ggacatccgc ttgcagctgg aaggggagtc ggggcgcatc aatggccgtt
      601 tcgccgagct ggactggacg ccgattctct acatccacaa gcagtacgag cgctccgtgc
      661 tggccgcgct gtttcgtacg gcgcatgtcg gctacgtcac gccgttgcgc gacggcatga
      721 atctcgtcgc caaagagtat gtgtcggcac aggatccgga gaatccgggc gtgctcgtgc
      781 tgtcacgctt tgccggcgcg gcgcaggaac tggatggcgc gttgatcgtc aatccggtcg
      841 acatcgacgg gatggccgag gcgctagcca aggcgctgga gatgccgctt gtggaacgtc
      901 aggcgcgtca tcgcgacatg atggtgcagc tgcgcgagaa caatgtgtcg gtgtggcgcg
      961 ataacttcat gcgcgatctg cagagcgtgg atgcggcgaa gagcaccagg acggggaagg
     1021 tgcgtgctgg ctcggccagg aaagccgtgc gcgaaacctt ggcgggatag gtgggattca
     1081 ggcggcgatt caggccggaa tcaggccgga ttcgggccga ttgcatctac ccggaaaaac
     1141 agaaaagccg ctcgaaagcg gcttttctgt tttttgctga agtcgcagag ggcggcgcat
     1201 cgcttagacc acgtgctgct cgtccacttt cttgccgccg atatgcagcg tggtgccctt
     1261 gccatactgc ttcgaaagca ggtcgcggta gaggcctggg cgttggcgca acgcttccgg
     1321 actgccgtcg tcgatcacct tgccctggct catcacgatg atccgatcga agttgttcag
     1381 cgtcgacaac cggtgcgcga tcgcgatcac cgtgcggccc accatcagcc ggtcgagcgc
     1441 cttctggatc gcttcttccg atgcactgtc gagcgccgag gtggcctcgt cgagcagcag
     1501 gatcggggcg ttcttcagga tcgcgcgcgc aatcgcgatc cgctgacgct gtccgccgga
     1561 gagcttcacg ccacgatcgc cgacgatcgt gtcgaaccct tccggcatgg cttcaatgaa
     1621 gtcgctgcag cgggcttcgc gcgcggcggc gaccacttct tcgcggctcg cttcagggcg
     1681 gccgtaagcg atgttctcgt acacggtgcg atggaacagc gagatatctt gcggcaccag
     1741 cgcgatggcg tgacgcaagc tgtcctgggt gatcccggcg atctcctggc cgtcgatcct
     1801 gatcgcaccg ccctgcgtct cgtagaaacg ctgcagcagc gccagcacgg tggacttgcc
     1861 cgcgcccgac ttgccgatca ggccgacccg ctggcccggt tcgatatgca ggtcgaagtg
     1921 atcgagaatc ggccggcggc gcggataggc gaacgtcacg ctttcgaaat cgacccggcc
     1981 gccttgcggc acgagttcgc tcgcgccgtc gcggtcgggc atgccgtgcg gctcgagcag
     2041 cgtgcggaca gcttcggcga gccgcgcgac gtgctgggtg acgtcgacga gcgccacggc
     2101 cagatcgcgc gtgccgtgca ggatcgtgaa gcccagcgag ctgaccagca cgatgtcgcc
     2161 ggaggtcgcc ttgccttgat cccacagcca cagcgcccag cccagcaagc ccgccgacag
     2221 cagcgcggtg atgaccgcat gcagcaggcg cagcttttcc agatacagca ggctctgctg
     2281 gcgtgcttcc atttccgctt tgacggtcgc gccgaaacgc tgctgttcgc gaaacgtcat
     2341 gccgaaggcg cgcacgaggc ccatgttgct gatcacgtcg accagctcac cgtccaccga
     2401 cgccgccttg gtagcgaagt gatggtgccg ggccgaaccg cgccccgcca gcttgtaaag
     2461 cacgaccgac agaattgccg agcacagcat caggccgccc gccatcagcg gattcacggc
     2521 gatgatcatg atgattgcac cgagcaccgc gatacatggc ggcaacacgt tccaggcggt
     2581 ggtgttctcg gcggtgtaga cggcgttcga ggttgccgtg atgcggctcg ccagcatgcc
     2641 cggctgtttc tccgagtaat aggtgggtga gtggccgctc aaatactgga acaggtcgcg
     2701 ccgcaggtcg cccgtgaccg ccacgaaggt gtgcgcggca acccagccgc cgacccgcca
     2761 cagcaggttg tcggcggcga tcaggccgac caggatggcg aacgcacccc acagcggacc
     2821 ggggtgatgc cggccttcgc cgagcacgtc gatcagatgc ttgatcgcat attgagaagc
     2881 gagcgcacag cccacagccg cgaacacgct gcacaggacg atcgcatgcg cgagcggatg
     2941 gcggcggata aagcggaaaa gaaacgcaag cggccgatgc gcatagctcg cgagctttgc
     3001 gttcagggca ttacgctggg cgatggttaa gtgttccaat tgatcgtgag gtcgatgtgt
     3061 ttcggattgt gcatgcaagg catgggccac agcaaagcgt tcgctgcgaa gccgatgctt
     3121 cgcattgtaa acatccaaaa gcgattgcgc cctgtgtatc cgggcgatgg cgcgactgat
     3181 ttcgcgatag atgagcgcga cctactgttg ctcgagccgc cagcggaccg gtggttcacg
     3241 caggttgcgg actatttcag gatataatta caaattgcat tcaaccgcgg tgcggcattt
     3301 ctgcaatgtc tgccggtaaa gttgatgcac gagcttacag atgccggaat cgaggtctgc
     3361 gctgcaagcc agttgctact gtagaactgt cttagaaaac aagggtttgc aaagtgtttc
     3421 gcctttgtaa caacgtaaag actttgaaat gttgtggtgc agcgagattc ccggcactga
     3481 taatgcatgc tctgtaggcc cgtcgaaata tctgacagtt tgtcaaccta ggtgtcatgt
     3541 gtgcgtttac cggcacatgc gcagacggcg aacagctgat ctggctcact acccaagcga
     3601 tccatcgcag gatttctcga aagaatcagg cccgcgccgg catatccggc cagactgccc
     3661 tggcgagcaa gcgagtcgct tttaccaaac gcagtacctt tgcctgattt tttacgagga
     3721 gttcttcatt atgcgaatcg ctcaaatcgc tcccttgcac gaggcggttc ctcccaagct
     3781 ttacggtggt acggaacgcg tagtgtccta cctgaccgaa gcattggttg aacagggcca
     3841 cgacgtcacg ctcttcgcga gcggcgattc gcaaacctcg gcgaaactcg aagcgttctg
     3901 gccgcaggcg ctgcgcctcg acccgaccat ccgcgacgtg atggcgccgc acatgctgct
     3961 gctcgaagaa gtgcgccgcc gtgcggacga attcgacgtg ctgcatttcc acatcgatta
     4021 ctacccgttc tcgctgttcg cccgccagcc ggtgccgttc ctgaccacgc tgcacggccg
     4081 tctcgatctg ccggaactgc aaccgatctt caatacgttc agcgacgtgc cggtggtgtc
     4141 gatttcggac aaccagcgta gcccgctgca acaggcgaac tggctgcaaa ccgtttatca
     4201 cggcctgccg gaaaacgtgc tcacaccgat caaggacgtc gagccgggct acctcgcatt
     4261 cctcggccgc gtctcgccgg agaagggtct cgaccgcgcg atccgtatcg ccggccaggc
     4321 aggcatgaag ctcaaggtcg ccgccaagat cgacaaggcc gaccgtgcct attacgaaga
     4381 agtgatcaag ccgctgatgg cactcccgca cgtcgagtac attggcgaaa tcggcgaagc
     4441 cgaaaagcgt gagtttctcg gcaacgcgca tgcactggtg ttcccgatcg actggccgga
     4501 gccgttcggc ctggtgatga tcgaagccat ggcgtgcggt acgccggtcg tcgcgttcaa
     4561 tcgcggttcg gtgccggaag tgatcgaaaa cggcgtgtcg ggttttgtcg tcgaagacga
     4621 aatcagcgcg gttgccgccg tgaagcgtct gcacacgctg ccgcgcgcca aggtgcgtgc
     4681 cgcgttcgaa gcgcgcttct cgtcgaaggt gatggcgcag aactacgtcg ccacctacga
     4741 agcgctgctg cgtcagaagc atcgcacggt gctgcgcgaa gtcaacgcca gctaagtgct
     4801 ataaggaaag gcagtcggac cgtcttgccg gagcgccggc ggcgcttttt gctcggcgcc
     4861 ccccggcgat caggtggtcc ggctgtgccg aacaacaccc cgtgtgcgtc tcgcccacgg
     4921 ggcgttgttg catg
//
LOCUS       DATASH010000946         7971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4713011,
            whole genome shotgun sequence.
ACCESSION   DATASH010000946 DATASH010000000
VERSION     DATASH010000946.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7971
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4713011"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="VHV99_25280"
     CDS             complement(<1..880)
                     /locus_tag="VHV99_25280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638733.1"
                     /translation="MWIYISWMKMEWLHEFITSSFDNGLFEKSMIDLWLGKTSTDLTP
                     EAATYSSAVKAAFSDYQADKSTIDATRLSSEDRYGAELYQLSNSTGAPGSSPNAHSVI
                     PILIKIKAYETCRYRNVFGRPAEDSISSAVELESLFSLPATSREARATEVEFQKFLKS
                     ELRPRMFFDLDENCKKGCETAREIYIDSLKNEIAKHGGILNFSDISTSAKFTRNIIRT
                     TINDCRNFLYNYRGPTYRSACDEKSAKLLQSRYAAMYLSILAKRSREYLDILIGLRTR
                     LPSVSNADTNILLLDSR"
     gene            complement(1645..3360)
                     /locus_tag="VHV99_25285"
     CDS             complement(1645..3360)
                     /locus_tag="VHV99_25285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007745825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX3638734.1"
                     /translation="MPLASEPSCAAQTTGARLVVDALLTHGVERVFCVPGESFLAVLD
                     SLHDETDRIQTIVCRHEAAAANMAEAVGKLTGRPGVAIVTRGPGATHASIGVHTAFQD
                     STPMILLIGQCAREHLDREAFQEIDYRRMFGQMAKWVAQIDDPKRIPEYLSHAFHTAT
                     SGRPGPVVLSLPEDVLSDACELVAGAPGYKRVAASPSAVQMEELRRLLENAQRPMVIA
                     GGSGWTPDACADLQRFVENWQLPISLAFRFQDTLDNEHSNYAGDVGLGINPALAQRIR
                     DADLLLTLGPRLGEATTNGYTLLDIPKTKQTLVHVHQGAEELGRVYAADLPIVSGMPE
                     LAALLAELRPSSDKLAWAGAAEEAHREYLDWRKPRPIPGDVQMGEVIQQLRAHLPEDA
                     ILTNGAGNYATWLHRHFSYRHFRSQLAPTSGAMGYGVPAAIAAKSMYPQRAVVALAGD
                     GCFMMSAQELATAMQYDLHVIFIVVNNSHFGTIRMHQERHYPNRVHGTGLTNPDFAAF
                     ARSFGAHGETVERTNDFLPALQRSMKAGRAAVIEIRMPQEASTPGATLEQIREQGRKL
                     RATGA"
     gene            complement(3413..4801)
                     /locus_tag="VHV99_25290"
     CDS             complement(3413..4801)
                     /locus_tag="VHV99_25290"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015004074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HEX3638735.1"
                     /translation="MAVSVFDLFKIGIGPSSSHTVGPMRAALMFAQGLERDGLLGATA
                     SVKADLYGSLGATGKGHGTDRGVMLGLMGDAPDTVDPDTIAQRLEAVKASRKLALLGT
                     HEVPFVQKDHISFYRQALAEHPNGLKLHAFNAQGETLRESTYLSVGGGFVVTAGAPNT
                     KVLSAVEQLPHSFRSGNELLALCKSTGKSIAQLMWENERVWHTEEETRTGLLKIWDVM
                     QSCVSRGCGINNPDAEGNLPGPFQVKRRAPQLYRALSGNPELALRDPLSMVDWINLYA
                     IAVNEENAAGGRVVTAPTNGAAGIIPAVLHYYTRFMPGSNQQGVIDFLLTAAAIGILY
                     KLNASISGAEVGCQGEVGVACSMAAGALAAVMGGTPAQVENAAEIGMEHNLGLTCDPV
                     GGMVQIPCIERNAMGSVKAVNAARMALRGDGTHYVSLDSVIKTMMQTGADMKTKYKET
                     SRGGLAVNIVEC"
     gene            complement(4830..5960)
                     /locus_tag="VHV99_25295"
     CDS             complement(4830..5960)
                     /locus_tag="VHV99_25295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEX3638736.1"
                     /translation="MARKVRLMQSRTEAKRAQMGQQACMLLLAGAAVLLPLRQAQAAM
                     NFCAAPALQTSERTNADPGVKALVSNVQAHLNDPPHALAKLHTEGTLPHEGIYDQSVE
                     AEKDLDLLRDAALAWRATSDDRYLKLVDRLLYAWVTTYQPSFNPIDETHFDGLILAYD
                     MTASALPVKTRNASMAFLTKLANGYIAQIDAQPRPLKGTFKNNWQSHRIKLIAMAAFT
                     LDNRKMINAAQRLFDEHIGDNIAPDGSTFDFGERDALHYVTYDLQPLVTAALAARRHN
                     RNWLPEKAANGASLQAALNWLAPYADGSKTHEEFVHSSVPFDAKRREAGLPGYSGQWD
                     PKNATELFHLAARLDGRYTPIALQLAPTPPAWLAVCLPLAAR"
     gene            complement(6078..>7971)
                     /gene="gcvP"
                     /locus_tag="VHV99_25300"
     CDS             complement(6078..>7971)
                     /gene="gcvP"
                     /locus_tag="VHV99_25300"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015004072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEX3638737.1"
                     /translation="TAQALLAIMASMYAVYHGPHGLKTIALRVNRIAALLAEGAKQLG
                     YTLVNETFFDTLTFEGGARTQALHDAAKAKRINLRRVSDTQVGLSIDETTSRRDLADL
                     LAVFAEAAGAQDVPQVDALDAVVAASNTVSVPAALERTSAYLTHHVFNRHHSETEMLR
                     YLRSLSDKDLALDRSMIPLGSCTMKLNATSEMLPVTWPEFGQIHPFAPAEQTVGYRDM
                     IDQLEEMLVTATGYAAVSLQPNAGSQGEYAGLLIIHAYHASRGEAHRNVCLIPASAHG
                     TNPASAQMAGMQVVVVACDAQGNVDIEDLKKKADQHADKLAAIMITYPSTHGVFEANV
                     REICEIVHAHGGQVYVDGANMNAMVGLCAPGQFGGDVSHLNLHKTFCIPHGGGGPGVG
                     PVAVGAHLAQFLPNQISSGYERAPNGIGAVSGAPYGSASILPISWMYIAMMGAKNLTA
                     ATETAILNANYVAKKLAPHYPVLYSGPGGLVAHECILDLRPIKETSGITVDDVAKRLA
                     DYGFHAPTMSFPVPGTLMVEPTESESKEELDRFIEAMIAIREEIRAVEDGRSDREDNP
                     LKHAPHTAAVVIANDWKHAYARETAAYPLPTLIAKKYWPPVGRADNVYGDRNLFCSCV
                     PIADYE"
BASE COUNT         1503 a         2443 c         2374 g         1651 t
ORIGIN      
        1 cgcggctgtc gagcagcaga atgttggtat ctgcattaga cacagacggc agccgagttc
       61 tcaggcctat caaaatgtct aaatattccc gggaccgctt tgcaagtata gagagataca
      121 tggcggcata tctgctctgc aagagctttg cgctcttctc gtcgcacgct gagcgatagg
      181 taggtcctct ataattatat aaaaaattcc gacagtcatt tatcgttgtt cgaatgatat
      241 tgcgggtaaa ttttgcactt gtcgaaatgt cggaaaaatt caggattcca ccgtgtttgg
      301 ctatctcatt ttttaatgaa tctatatata tttcccgtgc tgtctcgcaa ccttttttgc
      361 agttctcgtc caggtcaaag aacatccgcg gccggagttc ggatttaagg aatttctgaa
      421 actctacttc ggtcgcacga gcctcgcgcg aggtggcggg taacgaaaac agcgattcca
      481 gttcaacggc ggaagaaata gaatcttctg cgggccgacc aaatacattg cggtaacgac
      541 aggtttcata cgcttttatt tttattaaaa tggggatgac gctatgggcg ttcggtgaag
      601 aaccaggggc gccagtcgaa ttggaaagtt ggtatagttc ggctccatag cggtcctctg
      661 aggataggcg tgttgcgtcg atagtgcttt tgtcagcttg atagtcggaa aaagctgctt
      721 ttacagcgct ggagtatgtg gccgcctcgg gcgtcaaatc agttgaagtc ttgcccagcc
      781 acaaatcaat catcgatttt tcaaataatc cattatcgaa gctactggta atgaattcgt
      841 gtagccactc cattttcatc caggatatat aaatccacat gtgatactcc tgattgtgtg
      901 aaccggtttg aaccaggctg tgagcagcca cgcggggtaa atcttcggca gtatcgggat
      961 ggtgaggacg cgcgttcagc ccgcccgctc tcctgctggc tcgcccagat agcgttgaag
     1021 aagcggttcc cgtcaggttg aacccagact ctttcgccct ctatgagcgc ccagtagtgg
     1081 ttctttgcca cctcgtacat acagcgttgg cgctgccccc agatgcatta aaagacgcac
     1141 ggtttcgacc gttccagacg tggagcgccg ggtgctcatc aatttcatcg tgctggcgtc
     1201 gcttcgttct ggtcgtggaa tcgttctctg cgtagctgcg cataagccgc ctccggatcg
     1261 gacagttgac tttgaataga accgtgagtg atccggtcgc gacgcgcgcc gtttccggca
     1321 acaggtcacc actacgcagc gaccctggtc gccaggggac gcggttctat caggaaaaat
     1381 ctgaacgagc gattgcctgc tcacgacggg taaccgcacg ttggcgaaga cgcacctgcc
     1441 ggtgcttcgc atgaatcgca ctgaaccctc gacagcgtcg aagattagtg gaggtgaatt
     1501 gaaatatagc cagtgcgcat gaacatctac gtgatggcgg gatgataatt ccgtcatgag
     1561 acggttcagc ggcgccgcgg cgacagcgct gcgacatcgc gcgcagcgct gtcgccgtcc
     1621 agtaactgcg aaagtggagc gccactaagc tccggttgct cgcaatttcc tcccttgctc
     1681 gcgaatctgc tccaacgtag cgcccggtgt actcgcctcc tgcggcatgc gaatctcgat
     1741 cactgctgca cgcccggctt tcatcgaacg ctgcaacgcc ggtaggaaat cgttcgtgcg
     1801 ctccaccgtc tcaccatgcg cgccgaacga cctcgcaaac gccgcgaagt cgggattcgt
     1861 gagaccggtg ccgtgcacac gattcggata gtgccgttcc tgatgcatcc gtatcgtgcc
     1921 gaagtggctg ttgttcacga cgatgaaaat cacatgcaag tcgtactgca tcgccgtcgc
     1981 gagttcttgc gcggacatca tgaagcagcc gtcgcctgct aacgcgacta ctgcgcgttg
     2041 cggatacatc gacttcgccg cgattgccgc gggtacaccg tagcccattg cgccactggt
     2101 cggcgcgagt tgcgagcgga aatgccgata cgaaaaatgc cgatgcagcc acgttgcgta
     2161 attgcccgca ccgttcgtga ggattgcgtc ttccggtaga tgcgcgcgca actgctggat
     2221 gacttcgccc atttgcacgt cgccgggaat cgggcgtggc ttgcgccagt cgagatactc
     2281 gcgatgcgct tcttcggcgg cgccggccca tgcgagcttg tcggacgacg gcctcaactc
     2341 ggcaagcaac gccgccagtt caggcatgcc ggagacgatc ggcaggtccg ccgcatagac
     2401 ccgcccgagt tcttccgcac cttgatgcac gtgcaccagc gtttgcttcg tcttcgggat
     2461 atcgagcaac gtgtagccgt tcgttgtcgc ttcgcctagc cgtggaccca acgtgagcag
     2521 caagtccgca tcgcggatac gttgagccag cgccggattg ataccgaggc cgacatcacc
     2581 cgcataattc gaatgctcgt tgtcgagtgt gtcctgaaag cggaacgcga ggctgatcgg
     2641 caattgccaa ttctcgacga agcgttgcag atcagcgcat gcgtcgggcg tccaaccgct
     2701 gccgccggca atcaccatcg gccgttgcgc gttttccagc aatcgacgca actcttccat
     2761 ctgcaccgca gacggcgacg ccgccacacg cttatatccc ggtgcgccgg ccacaagctc
     2821 acacgcgtca ctcaacacat cttccggcaa cgacaacacg actggccccg gccgccccga
     2881 tgtcgccgta tgaaacgcat ggctcagata ttcgggaatg cgcttcggat cgtcgatctg
     2941 cgcgacccac ttcgccatct gaccgaacat gcgccgatag tcgatttcct gaaaggcctc
     3001 gcgatcgaga tgctcacgcg cgcattggcc gatcagcagg atcatcggcg tcgagtcctg
     3061 aaacgcggtg tgcacgccaa tcgacgcatg cgtcgcgccg ggtccgcgcg tgacgattgc
     3121 gacgcccgga cgaccggtca atttcccgac cgcttcggcc atgttcgctg ctgccgcttc
     3181 gtgccggcaa acgatggtct ggatacgatc ggtttcgtcg tgtagcgaat cgagtacagc
     3241 gagaaagctc tcgccgggta cacagaaaac acgttcgacg ccatgcgtca gcaaggcatc
     3301 gacgacgaga cgcgcgcctg tggtttgggc ggcgcaagaa ggttcagaag cgagcggcat
     3361 ggcgatcaag cctccagatg ttgcgatagt tgcgatgggc gcagcggtgc cctcaacact
     3421 ccacgatatt caccgccaag ccaccgcgcg acgtttcctt gtacttcgtc ttcatgtccg
     3481 cgccggtctg catcatcgtc ttgatcacgg agtccagcga cacgtaatgg gtaccatcac
     3541 cgcgcagcgc cattcgcgcg gcgttcaccg ctttgaccga gcccatcgca ttgcgctcga
     3601 tacacggaat ctgcaccatc ccgccgaccg gatcgcacgt cagcccgagg ttatgctcca
     3661 tgccgatctc ggcagcgttt tccacttgcg caggcgtacc acccatcacc gccgcgagcg
     3721 caccggcggc catcgaacag gccacgccca cttcgccttg gcagcccact tccgcgcctg
     3781 aaatcgacgc gttcagcttg tacagaatgc cgatcgccgc agccgtcagc aggaagtcga
     3841 tcacgccttg ctgattcgag cccggcatga aacgcgtgta gtaatgcagc accgccggaa
     3901 tgatgccggc cgcaccattg gtcggagccg taaccacgcg cccgcccgcg gcgttctctt
     3961 cattcacggc gattgcgtag agattgatcc agtcgaccat cgacagcgga tcgcgcagcg
     4021 ccagctcagg attaccggac aacgcgcgat acaactgcgg tgcccggcgc ttcacctgga
     4081 acggaccggg caagtttcct tccgcatccg gattgttgat gccacaaccg cgcgacacgc
     4141 acgactgcat cacgtcccag atcttcagca agcccgtacg cgtctcctct tccgtgtgcc
     4201 agacgcgctc gttttcccac atcaattgcg cgatgctctt gccggtcgat ttgcacagcg
     4261 cgagcaattc attgccgctg cgaaacgaat gcggcaattg ttcgacggcg ctcagcacct
     4321 tcgtgttcgg cgcgcccgcc gtcaccacga aaccgccgcc caccgataga tacgtcgact
     4381 cgcgcagcgt ctcgccttgc gcattgaacg catgcagctt gagaccattc ggatgctcgg
     4441 ccagtgcctg gcgataaaac gaaatgtgat ctttctgcac gaacggcacc tcgtgcgtgc
     4501 cgagcaaagc gagcttgcgc gaggccttca ccgcctccag acgctgcgcg attgtgtcgg
     4561 gatcgaccgt atcaggcgca tcgcccatca agccgagcat cacgccgcga tcggtgccgt
     4621 gccccttgcc ggtcgcgcca agcgaaccgt acaaatccgc tttcaccgat gccgtcgccc
     4681 caagcagccc gtcgcgttca agtccttgcg cgaacatcag cgccgcgcgc atcggcccga
     4741 ctgtatggga actggacgga ccaatgccga ttttgaagag gtcgaacacg ctcactgcca
     4801 tttactgctc cctgctgata aaagttggac tagcgcgccg ccagcggcaa acacacggcg
     4861 agccacgcgg gcggcgtcgg tgcgagctgc agcgcgatcg gcgtatagcg tccatcgaga
     4921 cgcgccgcca ggtgaaatag ttctgtcgcg tttttcggat cccattgacc cgagtagcca
     4981 ggcaagccag cctcgcgacg tttggcatcg aacgggacgc tcgaatgcac gaattcttca
     5041 tgggtcttgc tgccgtccgc atacggcgct agccagttga gcgcggcttg cagtgacgcg
     5101 ccgttggcgg ccttctccgg caaccagttg cggttgtggc ggcgcgcggc cagcgcggcg
     5161 gtcacgagcg gttgcaggtc gtaagtgacg tagtgcagcg cgtcgcgctc gccgaagtcg
     5221 aacgtcgagc cgtccggcgc gatgttgtcg ccgatatgct cgtcgaacag acgctgcgcc
     5281 gcgttgatca tcttgcggtt gtcgagcgtg aacgcagcca tggcgatcag cttgatccgg
     5341 tggctctgcc agttgttctt gaacgtgccc ttgaggggcc gcggctgcgc gtcgatctgc
     5401 gcgatatagc cgttggcgag cttggtgagg aacgccatcg atgcattgcg cgttttcacc
     5461 ggcaacgcgc tcgcggtcat gtcgtaggcg aggatcaggc cgtcgaaatg ggtttcgtcg
     5521 atgggattga agctcggctg gtaagtcgtg acccacgcat acagcaaacg gtcgaccagc
     5581 ttcagataac gatcgtcgct cgtggcgcgc caggcgagcg ccgcatcgcg cagcagatcg
     5641 agatcctttt ccgcttcgac gctctgatcg tagatgccct cgtgcggcag cgtgccttcg
     5701 gtatgcagct tcgccagcgc atgcggcggg tcgttcagat gcgcctgcac gttgctcacc
     5761 agcgccttga cgcctggatc ggcattcgtg cgctcgctgg tttgcagggc gggcgccgca
     5821 caaaaattca tcgcggcctg cgcctgccgc agcggcagca gcacggccgc gcctgccaac
     5881 agcagcatgc aagcctgttg ccccatttgg gctcgcttcg cttcggtccg gctttgcatc
     5941 aatcgcacct ttcgcgccat gcgaaactcc tcgttaggct gattcggtgt gtgatgttag
     6001 ccgtttgcgg acgccagcga cagaaccgcg caacgcctgc ttacaacctt caggcattgc
     6061 gcggtttgcg gacacgctta ctcgtagtcg gcgatcggca cgcaggagca gaacagattg
     6121 cggtcgccgt acacgttgtc cgcacggcca accggcggcc agtacttctt cgcgatcagc
     6181 gtgggcagcg gataggcggc ggtttcgcgc gcatacgcat gcttccagtc gttcgcgatg
     6241 acgaccgctg ccgtatgcgg cgcgtgcttg agcggattgt cctcgcggtc cgagcggccg
     6301 tcttcgacag cacggatttc ctcgcggatc gcgatcatcg cttcgatgaa gcggtcgagt
     6361 tcttccttcg attccgattc ggtcggctcg accatcagcg tgcccggcac cgggaagctc
     6421 atggtcgggg cgtggaagcc gtagtcggcc aggcgcttgg cgacgtcgtc cacggtgatg
     6481 ccgctcgttt ccttgatcgg acgcagatcg agaatgcact cgtgcgcgac caggccgccg
     6541 gggcccgaat acagcacggg gtaatgcggc gcgagcttct tcgcgacgta gttcgcgttg
     6601 aggatcgcgg tttcggttgc cgccgtgagg ttcttcgcgc ccatcatcgc gatgtacatc
     6661 cacgagatcg gcaggatcga cgcggagccg tacggtgcgc cggacaccgc accgatgccg
     6721 ttcggcgcgc gctcatagcc cgacgagatc tggttcggca ggaattgcgc gagatgcgcg
     6781 ccgaccgcga ccggaccgac gcccggtccg ccgccgccgt gcggaatgca gaaggtcttg
     6841 tgcaggttca ggtgcgagac gtcgccgccg aactgccccg gtgcgcacag gccgaccatg
     6901 gcgttcatgt tggcgccgtc cacatagacc tggccgccgt gcgcatgcac gatctcgcag
     6961 atttcgcgga cgttcgcttc gaacacgccg tgcgtggacg gatacgtgat catgatcgcc
     7021 gcgagcttgt cggcgtgctg atcggctttc ttcttcaggt cttcgatatc gacgttgcct
     7081 tgcgcgtcgc acgcgacaac gaccacctgc atgccggcca tttgcgccga cgccgggttc
     7141 gtgccgtgcg ccgaagcggg gatcaggcag acgttgcgat gcgcttcacc gcgcgacgcg
     7201 tggtaagcgt ggatgatcaa gaggccggcg tactcgcctt gcgagccggc gttcggttgc
     7261 aacgacaccg ccgcgtaacc ggttgcggtg accagcatct cttcgagctg atcgatcatg
     7321 tcgcggtagc cgacggtttg ctcagccggt gcgaacggat ggatctggcc gaattcgggc
     7381 cacgtgaccg gcagcatttc cgaggtcgcg ttcagcttca tcgtgcacga gccgagcggg
     7441 atcatcgagc ggtcgagcgc gaggtccttg tccgagaggc tgcgcagata gcgcagcatt
     7501 tccgtttccg aatgatgacg gttgaacacg tggtgcgtga ggtacgcgct ggtgcgctcc
     7561 aaagcggccg gcaccgatac ggtgttcgat gcggcgacga ccgcgtcgag cgcatcgact
     7621 tgcggtacgt cttgcgcgcc cgctgcttct gcgaagacgg cgagcagatc ggccagatcg
     7681 cggcgcgacg tggtttcgtc gatcgacaga ccgacttgcg tgtcgctcac acggcgcaaa
     7741 ttgatgcgct tcgctttcgc ggcgtcgtgc aatgcttgag tacgtgcgcc gccttcgaac
     7801 gtcagcgtat cgaagaacgt ttcgttgacg agcgtgtagc cgagttgctt cgcgccttcc
     7861 gcgagcaaag cagcaatgcg gttcacgcgc agcgcgatcg ttttcagacc gtgcgggccg
     7921 tgatagacgg cgtacatgct ggccatgatc gcgagcagcg cttgcgcggt a
//
LOCUS       DATASH010000947         2612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5897829,
            whole genome shotgun sequence.
ACCESSION   DATASH010000947 DATASH010000000
VERSION     DATASH010000947.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5897829"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..1390
                     /gene="eno"
                     /locus_tag="VHV99_25305"
     CDS             107..1390
                     /gene="eno"
                     /locus_tag="VHV99_25305"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX3638738.1"
                     /translation="MSAIVDIIGREILDSRGNPTVECDVLLESGTMGRAAVPSGASTG
                     SREAIELRDGEAGRYGGKGVLKAVEHINTEISEAIMGLDASEQAFLDKTLLELDGTDN
                     KSRLGANAMLAVSMAVAKAAAEEAGLPLYRYFGGSGAMQLPVPMMNIVNGGAHANNSL
                     DIQEFMIVPVSQPTFREALRCGAEVFHALKKILSDRGMSTAVGDEGGFAPNFGSNDEC
                     LSTILQAIEKAGYRAGEDVLLALDCAASEFYHDGKYQLAGEGLQLSSAEFADYLANLA
                     DKFPIVSIEDGMHESDWAGWKILTDKLGKKIQLVGDDLFVTNTRILKEGIEKGIANSI
                     LIKINQIGTLTETFAAIEMAKRAGYTAVISHRSGETEDSTIADIAVGLNAGQIKTGSL
                     SRSDRISKYNQLLRIEEDLGDIASYPGKSAFYNLR"
     gene            1498..1929
                     /gene="ftsB"
                     /locus_tag="VHV99_25310"
     CDS             1498..1929
                     /gene="ftsB"
                     /locus_tag="VHV99_25310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HEX3638739.1"
                     /translation="MRLVTAVLIVLLALIQYPLWWGHGGWLRVHELQQQLAQQLQKNA
                     DSKLRNERIQGEVQDLQNGTAAVEERARYEMGMVKDGEVFVQFVSPNAPLPNANTSSA
                     TTSTRGEVSAAPLHMVPNPESRARPDRKHGGKAAKDKKPAH"
     gene            complement(2180..2560)
                     /locus_tag="VHV99_25315"
     CDS             complement(2180..2560)
                     /locus_tag="VHV99_25315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638740.1"
                     /translation="MDIPTSLPHCRRTFTVLTALTGSAVLMGCVMDPPGPSPIYSRLP
                     ADQSGMASTAQPLTPEERARYDAIDRQVLSDQNQAIAAENAAQAYSRYYSPPVSVYGS
                     YYGGGWGHGWGTGVGVGYAPYWGW"
BASE COUNT          563 a          769 c          821 g          459 t
ORIGIN      
        1 cccgatctga acccaatggt cgtccaacgt gtctggttag aggctgtgct tcgtatgcca
       61 gacgccgttt caccgtaaga atccaacgtc atttcttgag gaaaccatga gtgctatcgt
      121 agatatcatc ggtcgagaga ttctcgattc gcgaggcaac cccaccgtcg aatgcgacgt
      181 gctgctcgag tcgggcacga tgggccgcgc tgcggtgccg tcgggtgcgt cgaccggttc
      241 gcgtgaagcg atcgaactgc gcgatggtga agccggccgt tacggcggca agggcgtgct
      301 gaaggccgtc gagcacatca acaccgaaat ctccgaagcg atcatgggcc tcgacgcttc
      361 cgagcaggcc ttcctcgaca agaccctgct ggaacttgac ggcacggaca acaagtcgcg
      421 cctcggcgcg aacgcgatgc tggctgtgtc gatggccgtc gccaaggccg ccgctgaaga
      481 agccggcctg ccgctgtacc gctacttcgg cggctcgggc gcgatgcagc tgccggtgcc
      541 gatgatgaac atcgtcaacg gcggtgcgca tgcgaacaac agcctggaca tccaggaatt
      601 catgatcgtg ccggtcagcc agccgacctt ccgcgaagca ctgcgctgcg gcgccgaagt
      661 gttccacgcg ctgaagaaga tcctgtcgga ccgcggcatg agcacggcgg tgggcgacga
      721 aggcggcttc gcgccgaact tcggcagcaa cgacgaatgc ctgtccacta ttctgcaagc
      781 tatcgagaaa gcgggctacc gcgccggtga agacgtgctg ctcgcactcg actgcgcagc
      841 aagcgagttc taccacgacg gtaagtacca gctggcgggc gaaggcctgc aactgtcgtc
      901 ggcggaattt gccgactacc tggcgaacct ggccgacaag ttcccgatcg tttcgattga
      961 agacggcatg cacgagagcg actgggcagg ctggaagatc ctcaccgaca agctcggcaa
     1021 gaagatccag ctggtgggcg acgacctgtt cgtcaccaac acgcgcatcc tgaaggaagg
     1081 catcgagaag ggcatcgcca actcgatcct gatcaagatc aaccagatcg gtacgctgac
     1141 ggaaaccttc gcggcaatcg aaatggccaa gcgcgccggc tacacggctg tgatctcgca
     1201 ccgctcgggc gaaaccgaag attcgacgat tgcggacatc gccgtcggcc tgaacgccgg
     1261 tcagatcaag acgggttcgc tgtcgcgttc cgaccgtatc tcgaagtaca accagttgct
     1321 gcgtatcgaa gaagacctcg gtgatatcgc gagctatccg ggcaagtcgg cgttctacaa
     1381 tctgcgctaa tgaccttttc gatagcgccg accactgagc cggttatcct tcgtttctga
     1441 ttgaccccgc cgccctgcgt ataacgcagg gcggcgtgta tttattgtgc ttacttcatg
     1501 cggcttgtca ctgctgtcct gatcgtacta ctggcgctga tccagtaccc gctctggtgg
     1561 gggcacggcg gctggttgcg cgtgcatgag ttgcagcagc agctggcgca gcaactgcag
     1621 aagaatgccg attcgaagct gcgcaacgag cgtatccagg gcgaagtcca ggatttgcag
     1681 aacggcacgg ctgcggtgga agagcgggcg cgctacgaaa tgggcatggt gaaggacggc
     1741 gaagtattcg tgcagttcgt ctcgccgaac gcgccgttgc cgaacgcgaa cacgtcttcg
     1801 gctaccacct cgacgcgggg tgaagtgtcg gcggcgccac tgcacatggt gccgaatccg
     1861 gagtcgcgtg cgaggccgga tcgcaagcat ggtggtaagg cggcgaaaga taagaagccc
     1921 gcgcactgag ttgattcagg ctggttgagt tcgcggacga acgcaaaagc aacggcagaa
     1981 acaaacgcaa aagcgaacgc aaaagcgaac gcaaaagcga acgcaaaagc gaacgcaaaa
     2041 gcgaacgcaa aagcgaacgc aaaagctctg ggaacgaaaa aggcgcggtg tgaaacccgc
     2101 gcctttttct ttgggcttgc gaaacccgca tctctgccgc gttgcggtga cgggtacgcg
     2161 agcgcgtgcc cgcttcggct taccagcccc agtacggcgc gtaacctacg ccgacacccg
     2221 taccccagcc gtgcccccaa ccgccgccgt agtagctgcc gtacacgctg acgggcggcg
     2281 aataataacg tgagtacgcc tgagcagcgt tctcggcggc aatggcctga ttctgatcgg
     2341 atagcacctg acggtcgatc gcatcgtagc gcgcgcgttc ctcaggtgtg agcggctggg
     2401 cggtgctggc catacccgat tggtcggccg gtagccggct ataaatcggc gacgggcccg
     2461 gtggatccat cacgcagccc atcagtacag cgctgcctgt gagggcggtc agcacggtga
     2521 acgtgcgcct gcaatgcggc aacgaagtag gaatgtccat ttttgatctc cattggtcgg
     2581 aggtatgagc agaacatgcg cagcgcgcac cg
//
LOCUS       DATASH010000948         4352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3720504,
            whole genome shotgun sequence.
ACCESSION   DATASH010000948 DATASH010000000
VERSION     DATASH010000948.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4352
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3720504"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            261..>4352
                     /locus_tag="VHV99_25320"
     CDS             261..>4352
                     /locus_tag="VHV99_25320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014252664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3638741.1"
                     /translation="MSEEGTPANLVTPSFLAGGGELGALIRAHDWTQTALGAPENWPQ
                     GLKIAIRIMLTSRQPIWIGWGDELLYFYNDAYKSIIGGKHPAMLGQPTQVVWHEIWAD
                     IEPLLDTALKGAEGTFVEQKLLIMERNGFPEETYYTFSYSPIPGDDGKAGGILCANSD
                     GTMQVVGERQLALLKELAAASPNGRDWREACELSARALQSNPQDLPFALLYAGEPGSS
                     TVTLVGSCGIEPGHPAAPESMSLVSAPVWPFADVLKRQLPQMVRGLTERFGMALPSGP
                     WHLAPEQAVILPVSAGSETAPAVLLIVGLNPCRLFDDAYRSFLNLTAGQIGAAIGYAQ
                     AYQEERRRAEALAEIDRAKTTFFSNISHEFRTPLTLMLGPLEELLAKPRLGASTDNPR
                     PGSDADDRALIEVTHRNGLRLLKLVNALLDFSRIEAGRIQIHTEPTDVAGFTAELASL
                     FQSAIEAAGLRLEVQIPPTPIVVQLDREMWEKVVMNLLSNAYKFTFFGTIRVVVRAVE
                     GGGAEVSVSDSGIGIAEDEMPRLFERFHRVAGAPGRSVEGSGIGLAMVQELVKLHGGT
                     VQVESRLGDGACFTVTLPHGAALSHAAHEAADEAVRAAMSKHARTYVDAALRWSPENE
                     ILADASSAMTPAGKAAPAVGPTAAARLLVVDDNADLRDYMSRILRTAGHTVQLASDGL
                     AALEAARAAPPDLILSDVMMPRLDGFGLLRELRADPELRDTPVLMLSARAGEEARVDG
                     IEHGADDYLTKPFSARELLARVAGNLQLARLRRETEMKLREESRTLEILNRVGSTVAA
                     ELDLNRAVQIVTDAATELTGAAFGSFFYNVVDQAGGSYMLYTLSGVPKEAFEKFPMPR
                     NTAVFAPTFNGEGIVRVDDITKDPRYGHNSPHRGMPEGHLKVCSYLAVPVQSRSGEVV
                     GGLFFGHPEPAVFTERAERIVAGIAAQAAIAIDNARLYQAAQTEIAERAKAQSALHDL
                     NETLERRVIETVADRDRLWELSEDLLVVADVEGRLQRVSPSWSSALGHNAQWLMSRSY
                     FDLVHPDDIETVRTHLAELRRTGVPVRYENRFKRLDGSWRWVAWRLALDPDTARIHGV
                     GRDVTADKETTEALRHAEEALRMAQKMEAIGKLTGGVAHDFNNLLQVIGGNLQLLAKD
                     VAGADKPEQRVRNALAGVARGANLASQLLAFGRRQPLAPKVVNLGRFVRGLDDMLRRA
                     LGDGVEIETVVSGGLWNTLVDPFQVENALLNLAINARDAMKGHGKLTIEAGNAALDDV
                     YATRNAEVTPGQYVMLAVTDTGSGMSPEVRERVFEPFFTTKPEGQGTGLGLSMVYGFV
                     KQSGGHVKIYSEQGHGTTIRIYLPRVRQEEDLETNIDAGPAKGG"
BASE COUNT          776 a         1390 c         1449 g          737 t
ORIGIN      
        1 gcggcccctg gagccgcgcg acctcgacct tgtgcggtcc tggcgcaatg aaagggttga
       61 tctaaaaacc ctttcatttc aagtctttca cgcgtcaccc caccttcctc cacaacgccg
      121 ctccgtgcga gaatcgcgct acgtcggacg gctgccaccg ggcagccgtc cggcgtgcct
      181 ggccttgttt ttgctattcc agtccgcacg aattcgaaac agcagcgtag gagatcgcgt
      241 tgaaaaagcc ggacagcgtc atgtcggagg agggaacgcc cgcgaacctc gtcacgccgt
      301 cgtttcttgc cggcggcggt gaactgggtg cgttgatccg cgcccacgac tggacgcaga
      361 cggcgctcgg cgcccccgag aactggccgc aagggctgaa gatcgcgatt cgcatcatgc
      421 tgacttcgcg tcagccgatc tggatcggct ggggtgacga gcttctctac ttttacaacg
      481 acgcctacaa atcgatcatc gggggcaagc acccggccat gctcggtcag cccactcaag
      541 tcgtgtggca tgaaatctgg gccgatatcg agccgctcct ggataccgcg ctcaaaggcg
      601 ctgagggcac cttcgtcgaa cagaagctgc tcatcatgga gcgcaacggc ttccccgagg
      661 aaacgtatta caccttctcg tacagtccga ttcccggcga cgacggcaag gccggcggca
      721 tcctctgcgc caatagcgac ggcaccatgc aggtggtcgg tgaacggcaa ctggcgctgc
      781 tgaaggaact ggcggcggcc agtccgaacg gccgcgactg gcgtgaggcg tgcgaactga
      841 gcgcgcgtgc gttgcagtcc aatccgcaag atcttccctt cgcgctgctg tatgcggggg
      901 agccgggcag cagcacggtc acgctagtcg gctcgtgcgg catcgagccg ggtcatccgg
      961 cggcgcctga atcgatgagc ctggtcagcg ccccggtgtg gccgttcgcg gacgtgctga
     1021 agcgccagct gccgcagatg gtgcgcggct tgacggagcg tttcggcatg gccttgccga
     1081 gcggcccgtg gcacctcgcg cccgagcaag cggtcatcct gcctgtctca gcgggtagcg
     1141 agacggcgcc ggcggtgctg ttgatcgtcg gtctgaatcc ttgccgtctt ttcgacgacg
     1201 cctatcgcag tttcctgaat ctgaccgctg gccagatcgg cgcggcgatc ggctacgcgc
     1261 aggcctacca ggaagagcgg cggcgcgccg aggcgctggc cgaaatcgac cgcgcgaaaa
     1321 ccaccttctt ctcgaacatc agccacgaat tccgcacgcc gctcacgctg atgctcggcc
     1381 cgctcgagga attgctcgcc aagccgaggc ttggcgcgag cacggataac cctcgtcccg
     1441 gcagcgatgc cgacgaccgc gcgctgatcg aggtcacgca tcgcaacggc ttgcgtctgc
     1501 tgaaactcgt caacgcgctg ctcgactttt cgcggatcga ggcgggacgc atccagattc
     1561 acaccgaacc gaccgacgtc gccggcttca cggccgaact cgcgtcgctg ttccagtccg
     1621 ctatcgaggc agccggcttg cggctcgagg tgcagattcc gcccacgccg atcgtcgtgc
     1681 aactcgaccg cgagatgtgg gaaaaggtcg tgatgaatct gctgtcgaac gcgtacaagt
     1741 tcacgttctt cggcacgatc cgggtcgtgg tccgtgctgt cgagggcggc ggtgccgaag
     1801 tcagcgtgag cgactccggt atcggcattg cggaagatga aatgccgcgc ctcttcgagc
     1861 gctttcatcg tgtggcgggt gcacccggcc gctcggtcga gggcagcggc atcggtctcg
     1921 cgatggtgca ggagctcgtg aagctgcacg gtggcaccgt acaagtcgaa agtcgtctgg
     1981 gcgacggcgc gtgctttacg gtcacgctgc cgcacggcgc ggcgctgtct catgcggcac
     2041 acgaagccgc ggacgaagcg gtgcgcgccg ccatgagcaa gcacgcgcgc acctatgtcg
     2101 atgccgcatt gcgctggagt ccggagaacg aaattctcgc cgatgcgtcg tccgccatga
     2161 cgccggccgg gaaggcggca cctgctgtcg ggccaacggc ggccgcgcgc ttgctggtcg
     2221 tcgacgacaa cgccgacttg cgcgactaca tgagccgcat cctgcgcacg gccggccaca
     2281 ccgtccaact ggccagcgac ggcctggcgg cgctcgaagc ggcacgcgcc gcgccgcccg
     2341 acctgatcct gtccgacgtg atgatgccgc gcctcgacgg cttcggcctg ctgcgtgaac
     2401 tgcgcgccga tcccgagttg cgcgacacgc cggtactgat gctgtccgcg cgcgcgggcg
     2461 aagaggctcg tgtggacggt atcgagcacg gtgccgacga ttatctgacc aagccatttt
     2521 ccgcccgcga actgctcgcg cgcgtggcgg gcaatttgca actcgcacgg ctgcgtcgcg
     2581 agaccgagat gaagttgcgc gaggaatcgc gcacgctcga aattctcaac cgcgtcggct
     2641 cgacggtcgc ggccgaactc gatctgaacc gcgcggtgca gatcgtgacc gacgccgcca
     2701 cggaattgac cggcgccgca tttggttcgt tcttctacaa cgtagtcgac caggcgggcg
     2761 gcagctatat gctgtacacg ctctccggtg tgccgaaaga agcgttcgag aagtttccca
     2821 tgccgcgcaa cacggcggtg ttcgcgccga cgttcaatgg cgaaggcatc gttcgcgtcg
     2881 acgacatcac caaagatccg cgttatgggc acaactcgcc gcatcgtggc atgccggaag
     2941 gccacctgaa ggtgtgcagc tatctggccg tgccggtgca atcgcgcagc ggcgaagtgg
     3001 tcggcggctt gtttttcggc catccggaac ccgctgtgtt caccgagcgt gcggaacgga
     3061 ttgtggccgg tatcgcggct caggcggcga ttgcgattga caacgcgcgc ctctatcagg
     3121 cggcgcaaac cgaaattgcc gaacgcgcca aggcgcaaag cgcgctgcat gatctgaacg
     3181 agacgctcga gcgccgcgtg atcgagacgg tggcggaccg cgaccggctg tgggagctga
     3241 gcgaggacct gctcgtggtg gccgacgtgg aaggccgctt gcagcgcgtg agcccgtcgt
     3301 ggagcagcgc cctcggacac aatgcgcaat ggctgatgtc gcgttcctac ttcgatctgg
     3361 tccatccgga cgacatcgag accgtgcgca cgcacctggc cgaactgcgc cgcacgggtg
     3421 tgccggtgcg ctacgaaaac cgcttcaagc ggctcgacgg cagctggcgc tgggtcgcct
     3481 ggcggctcgc gctcgatccg gacaccgcgc ggattcacgg cgtgggccgc gacgtcaccg
     3541 ccgacaagga aaccaccgag gcgctgcgcc acgcggaaga agcgctgcgc atggcgcaga
     3601 agatggaggc gatcggcaag ctgacgggcg gcgtcgcgca cgatttcaac aacctgctgc
     3661 aagtgatcgg cggcaacctg caattgctcg cgaaagacgt cgccggcgcc gacaagcctg
     3721 agcagcgcgt gcgcaatgca ctggcgggcg tggcgcgcgg cgcgaatctc gcgtcgcaat
     3781 tgctggcgtt cgggcggcgc cagccgcttg cgccgaaggt ggtcaatctc ggccgcttcg
     3841 tgcgtggact cgacgacatg ctgcggcgcg cgcttggcga tggcgtcgaa attgaaacgg
     3901 tcgtgtccgg tggtttgtgg aatacgctgg tcgatccctt ccaggtcgaa aacgcgctgc
     3961 tcaatctggc gatcaacgcg cgcgacgcga tgaaaggcca cggcaagctc acgatcgaag
     4021 cgggcaacgc tgcgctcgac gatgtctacg ccacgcgcaa tgccgaagtc acgccgggcc
     4081 aatacgtgat gctcgcggtc acggataccg gctcggggat gtcgccggaa gtgcgcgagc
     4141 gcgtgttcga accgttcttc acgaccaagc cggaaggaca gggcacgggt ttgggcctga
     4201 gcatggtgta tggcttcgtg aagcagtccg gcggccatgt gaagatctac agcgagcaag
     4261 gccacggtac gacgattcgc atctatctgc cgcgcgtgcg tcaggaagaa gacctcgaaa
     4321 cgaatatcga cgccgggccc gcgaagggcg gc
//
LOCUS       DATASH010000949         2826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1350201,
            whole genome shotgun sequence.
ACCESSION   DATASH010000949 DATASH010000000
VERSION     DATASH010000949.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1350201"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="VHV99_25325"
     CDS             <1..395
                     /locus_tag="VHV99_25325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016346070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX3638742.1"
                     /translation="MLQPGDMLHVDSRDDNTVYVMGEVTKPTAVSPLRDGTLTLGEAI
                     VQAGQINQDTSNASQLFVVRQATGDSPVIYRLDARSPVSMLLANQFPLEAKDVVYVDN
                     SGLVRANRVLNLLLPAINAGLTAAIVTK"
     gene            483..2717
                     /locus_tag="VHV99_25330"
     CDS             483..2717
                     /locus_tag="VHV99_25330"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016346069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEX3638743.1"
                     /translation="MAINFENRYADVVSTGDELRLSDYLAAILGSWRIIAMMTLVVVA
                     LGTAYAFIASPVYRADAMIQVTESNPGNNANNANANAVQTVSQMFDAKSTTAAEIELL
                     RSRLVVEDTVNKLHLDISARPRYFPIVGGMIGDILGTRNGQAPVSWLSGFAWGSESIS
                     VPKFDTPKEAYDKKYILTAGENGSYVLSDPDGIAFLNGKVGQEASGTSALGPVTLLVE
                     NMSGEPGAKFELSRASTLGTVDALQKRLTVAETTLQSGIIALSLEGHDSKLTSQIVNN
                     IARRFIQQDLDTRSAEAGKTLAFLEQQLPEVRAQLDQAEQKYNNFRNKQGTVDLSEES
                     RLLLQQVVDNKSKLMDLQQQRAELSQRFTANHPSVAALDAQIGALQAAQGSLTKNVSV
                     LPDTEQTALRLLRDVRVDTELYTNLLNSAQQLRVAKAGQVGNVRVVDFAEAADDPVRP
                     KRLLVILISLAGGLVLGILVAFIRKSLYGGVERPEEIENRLGVRVFAIVPRSSQQLRL
                     QQKVGSRSQGLHVLAATSPEDAAVEGIRGLRTSLQLQMADARNNVVMLTGSRPDAGKS
                     FLSVNLATLVASTRKRVLLIDGDMRRGDVHSHFGVRHMPGLSDVLMGSDIESAVLHDV
                     LPGVDLLTKGSLPSHPSELLMSDRMHEVLEVLKHKYDLVIIDTPPVLAVTDATVIGKH
                     AGTSLLVVRHGRNQVEEIGETMKRLQHGGVNMKGVLLTDVPQSRLMSGSAYTNYYGYE
                     SIPE"
BASE COUNT          573 a          835 c          907 g          511 t
ORIGIN      
        1 tcatgctgca gccgggcgac atgctgcatg tggactcccg cgacgacaac accgtctacg
       61 tgatgggcga ggtgaccaag ccgacggccg tgtcgccgtt gcgcgacggc acgctcacgt
      121 tgggtgaagc aatcgtccag gcgggccaga tcaaccagga cacctccaat gcgtcgcagc
      181 tgttcgtggt tcgccaggca accggcgatt cgccggtgat ctatcgcctc gatgcacgct
      241 cgccggtgtc catgctgctc gcaaaccagt ttccgctcga ggcgaaggat gttgtctatg
      301 tcgacaacag tggtcttgtc cgcgccaacc gcgtgttgaa cctgttgttg ccggctatca
      361 acgcgggcct gacggccgcg atcgtaacca agtagtcctt ggagctgcgt ccggcgctcg
      421 gacgtcgtga agacatcagg cgccggcccg tttcgattgt tcgtttgagt gagcgacaaa
      481 aaatggccat caattttgaa aaccgctatg cggatgtggt gtcgacaggg gacgagctgc
      541 gcttgtccga ctacctggcg gccatcttgg gaagctggcg aatcatcgcc atgatgaccc
      601 tcgtggtggt cgcactcggc acggcctacg cattcattgc gtcgccggtt tatcgcgcag
      661 acgcgatgat ccaggtgacc gagagcaatc cgggaaataa cgccaataac gccaatgcga
      721 acgcggtgca gaccgtgtcg caaatgttcg atgcgaagtc gacgacggcg gccgaaatcg
      781 agttgctgcg ctcaagactg gtggtcgagg acacggtgaa caagctgcat ctggatatca
      841 gcgcgcggcc gcgctatttc ccgatcgtgg gcggcatgat cggcgacatc ctgggcacgc
      901 gcaatggtca ggcgccggtg agctggctgt cgggtttcgc atggggcagc gaaagcatct
      961 cggtgccgaa gttcgacacg cccaaggaag cctacgacaa gaagtacatc ctgacggccg
     1021 gcgaaaacgg cagctatgtg ctgagcgacc cggacggcat tgcctttctg aacggcaagg
     1081 tcggccagga agcgagcggt acgagcgcgc tcgggcccgt cacgctgctg gttgaaaaca
     1141 tgagcggcga gccgggtgcc aagttcgaac tgtcgcgtgc ttcaacgctc ggtaccgtgg
     1201 acgcgttgca aaagcgcctg acggtggctg aaacaacgct gcagtccggc attatcgcgt
     1261 tgagccttga aggccacgac agcaagctga cgtcgcagat cgtgaacaac attgcgcgcc
     1321 gcttcattca acaggatctc gatacgcgtt cggcggaagc cggcaagacg ctcgcgttcc
     1381 tcgagcagca gttgccggaa gtgcgcgcac aactcgacca ggccgagcag aagtacaaca
     1441 acttccgcaa caagcaaggc acggtcgacc tgagcgaaga aagccgcctg ttgctccagc
     1501 aagttgtcga caacaagtcg aagctgatgg acctgcaaca gcaacgcgcc gaactttcgc
     1561 agcgtttcac cgcgaaccat ccgtcggtgg ccgcgctcga cgcgcagatc ggtgcattgc
     1621 aggcggctca aggttcgctt accaagaacg tttccgtgct gcccgatacc gaacaaaccg
     1681 cgctgcgcct gctgcgcgac gtgcgggtgg ataccgagct ctatacgaac ctgcttaaca
     1741 gcgcgcagca gttgcgcgtg gcgaaggctg ggcaagttgg caacgtgcgg gtggtcgact
     1801 tcgctgaagc ggctgacgac cctgtgcgcc cgaagcgcct gctcgtgatc ctgatcagcc
     1861 tggccggtgg cctggtgctc ggcatcctcg tggcgttcat ccgcaagtcg ctgtatggcg
     1921 gcgtggaacg tccggaagaa atcgaaaacc gcctgggcgt gcgtgtgttc gccatcgtgc
     1981 cgcgcagcag ccagcaattg cgcttgcagc agaaggtcgg ttcgcgtagc cagggtcttc
     2041 acgtcctcgc cgcgacctcg ccggaagacg cggcagtgga agggattcgc ggcctgcgca
     2101 cgtcgctgca actgcagatg gccgatgcac gcaacaacgt ggtcatgctg acgggttcgc
     2161 gcccggacgc cggcaagtcg ttcctctcgg tcaacctggc aacgctggtg gcatcgacga
     2221 gaaagcgtgt actgctgatc gacggcgaca tgcgtcgcgg cgacgtccac tcgcacttcg
     2281 gcgtgcgcca tatgccgggc ttgtccgacg tgctgatggg ctcggatatc gaatccgcgg
     2341 tgctgcatga cgtgctgccg ggcgtcgacc tgctgaccaa gggttcgttg ccgtcgcatc
     2401 cgtcggaact gctgatgagc gaccgcatgc atgaggtgct ggaagtactc aagcacaagt
     2461 acgacctcgt gatcattgat accccgcctg tgctggcggt caccgacgca accgtgattg
     2521 gcaagcatgc cggcacgagc ctgctggtgg tgcgccatgg ccgcaaccag gtggaagaga
     2581 tcggcgaaac gatgaagcgc ctgcagcacg gcggcgtgaa catgaagggc gtgctgctga
     2641 ccgatgtgcc gcagtcgaga ctgatgtccg gcagcgcgta cacaaactac tacggctacg
     2701 agagcatccc tgaatgatgc ctggacggga agaccttgcg gcgcgcggcg agtttgatgt
     2761 cggcgccgca catccaatct gaaaagacga gaggtacacg atgaaacgca aggtcgcttt
     2821 gatcac
//
LOCUS       DATASH010000950         2129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2733217,
            whole genome shotgun sequence.
ACCESSION   DATASH010000950 DATASH010000000
VERSION     DATASH010000950.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2733217"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            291..593
                     /locus_tag="VHV99_25335"
     CDS             291..593
                     /locus_tag="VHV99_25335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018431982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI-like protein"
                     /protein_id="HEX3638744.1"
                     /translation="MHFLLMYELSADYLERRAAFRDRHLTAAWVSVERGELLLGGALE
                     APIDRAMLLFEADSPAVAEAFARADPYVTEGLVTRWEVRKWNTVAGEGAAQPVRPT"
     gene            complement(633..1676)
                     /locus_tag="VHV99_25340"
     CDS             complement(633..1676)
                     /locus_tag="VHV99_25340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016354482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3638745.1"
                     /translation="MTDRVHAMRVFIRVVDTNSFSRAAESLGMPRASVSTTIQQLEAL
                     LGVQLLARTTRRLNLTAEGAECYERCVRILADIDDLEAGFHGRDERLRGRLRIELPDT
                     VATALVVPALPDFHARYPHIELSIGICNRAVDLVGEGVDCSVQLGELPDSGLIARRLG
                     SFEHVTCATPAYLEAHGTPKTLDELAQHIAVNCVSPRTGRPCDFDFEVDGEAVTVKMA
                     GFVQVTDEHAYLACGLQGLGLIQPPRIAALPYLRSGQLREVLPQWKSMPMNVSVAFVK
                     SRQAAPRVSAFVDWLAELFERSQEMDDTLTRLFSTARRPAVASASASARAAAPRPAAA
                     RDEDAHEETTSES"
     gene            complement(1824..>2129)
                     /locus_tag="VHV99_25345"
     CDS             complement(1824..>2129)
                     /locus_tag="VHV99_25345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX3638746.1"
                     /translation="MVRMMEPQSEGIPVEVYCFTAVTAWTQYERIQGDVFDHLLSILP
                     EMGLRLYQAPSGADMSGLVSRATGGSAIGAIGASSAIGPSAMGGADSGRLRGEFPTL"
BASE COUNT          408 a          640 c          677 g          404 t
ORIGIN      
        1 aggcgaaaaa gccgcggaac aggcatgctc cggagtatgt ccgacgctgc cggaatattg
       61 agtgtcgcgg gtaattcgcg aatctgcgcg aaggtaaaag tcgaaagtgt cgccatgatc
      121 gcacacacgg cctcaggcat acagcttgac gtaactacca aatcttacac gcgcgtatta
      181 cagtgcttat aagcggcacg atcgtgctat ttaattcgat ttcattcgat ttcattcgat
      241 ttcaaacgac cgcgacgcgc tccgcgtcgc ctgcaatggg agacaagctt atgcactttc
      301 tattgatgta cgaattgagc gccgattatc tcgaacgccg ggcagcgttt cgtgaccgcc
      361 atctgacggc ggcgtgggta tcggtggagc gtggggaatt gttgctgggc ggggcgctgg
      421 aggcgcccat cgaccgggcg atgctgctgt tcgaggcgga ttcgcccgcc gtggcggagg
      481 cgttcgcgcg ggccgatcca tatgtgaccg aggggttggt cacgcgctgg gaagtgcgca
      541 agtggaatac cgtggcgggc gaaggggctg ctcagccggt caggccgacg tgagcgcccc
      601 cgcgcccggg tagcggtcat gcatgcggcg cctcatgact cgctggtggt ttcttcgtgc
      661 gcgtcctcgt cgcgggctgc ggccggcctt ggagccgcgg cccgcgcgga tgcggaagcg
      721 gatgcgaccg ccgggcggcg agccgtgctg aacaggcgcg tgagcgtatc gtccatttcc
      781 tgcgagcgct cgaacaattc cgccagccag tccacgaacg cgctgaccct cggcgcggct
      841 tgccggctct tgacgaatgc caccgataca ttcattggca ttgacttcca ttgcggcagc
      901 acttcccgca gttgcccgga acgcaggtac ggtaacgcgg caattcgtgg cggttgaatc
      961 agcccgagcc cttgcaggcc gcacgcgaga taggcgtgtt cgtcggtgac ctgaacaaat
     1021 cccgccattt tcacggtcac cgcttcacca tccacttcga agtcgaaatc acacggccgg
     1081 cccgtgcgag gcgacacgca gttcaccgcg atgtgctgcg cgagctcgtc gagggtcttc
     1141 ggcgtgccgt gcgcttccag atacgcgggt gtcgcacagg tcacgtgctc aaagctgccg
     1201 aggcgccgcg cgatcagccc ggaatcgggc aattcgccaa gctgcacgct gcaatccacg
     1261 ccttcgccga ccaggtcgac ggcgcgattg cagataccga tcgacaactc gatatgcgga
     1321 taccgcgcat ggaaatcggg cagcgcgggc acgaccagcg cggtggcgac cgtatcgggc
     1381 aactcgatgc gcagacggcc gcgcagacgc tcgtcgcgac cgtgaaagcc cgcttccaga
     1441 tcgtcgatat ccgcgagaat gcgcacgcag cgctcgtaac actcggcccc ttcggccgtg
     1501 agattgagac gccgcgtggt gcgtgcgagc agttgcacgc cgagcagcgc ctcgagttgt
     1561 tgaatggtcg tcgagacgct tgcacgcggc atgccgagag attcggcggc gcgcgagaag
     1621 ctgttggtat cgacgacacg gatgaaaaca cgcatagcat gaacgcgatc ggtcatggga
     1681 gttttcctgt agaagcgatg aatgcgatga ccggcgggca ccttcggttg atgcagaagg
     1741 aatgccggca aggttcacac tttaggcatc aggacggttc gcgtgaatac ataaaagtcg
     1801 cagattcttg cctgcttctc cagctagagc gtggggaact cgccgcgcaa acggccgctg
     1861 tccgcgccgc ccatggcgct tgggccgatt gcactgcttg cgccgattgc gccgattgcg
     1921 ctacctccag tcgccctgct caccaatccg ctcatgtctg caccggacgg cgcttgatag
     1981 agccgcaggc ccatctccgg caggatagac agcagatggt cgaacacatc gccctggatg
     2041 cgttcgtatt gtgtccatgc agtgacggcg gtgaaacaat acacctcgac cggaatgcct
     2101 tccgattgtg gttccatcat gcgcaccat
//
LOCUS       DATASH010000951         3695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6489429,
            whole genome shotgun sequence.
ACCESSION   DATASH010000951 DATASH010000000
VERSION     DATASH010000951.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6489429"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..1658
                     /gene="garD"
                     /locus_tag="VHV99_25350"
     CDS             69..1658
                     /gene="garD"
                     /locus_tag="VHV99_25350"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006756361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HEX3638747.1"
                     /translation="MEQPPLYIRVHPNDNVAIVVNDGGLGEGAVFPDGLALRERVPQG
                     HKVALADLAEGDEVVRYNVVIGYALKALPKGSWINEHVIRMPSPPGLEDLPIATIKPP
                     AMPPLEGFTFEGYRNADGSVGSRNILAITTTVQCVADVVQHAVTRIKAELLPNYPNVD
                     DVVSLGHTYGCGVAIDAPDAMVPIRTVRNISLNPNFGGEVMMVSLGCEKLQPERLMPP
                     GTIPIAAAANVAEVADIGDIEADMNGGVVVLQDDSHVGFQSMIESIMRMADGHLKRLN
                     NRRRETCPASDLVIGVQCGGSDAFSGLTANPAVGFATDLLVRAGATVMFSEVTEVRDG
                     VDQLTARAANADVAAAIIREMQWYDDYLKRGGADRSANTTPGNKKGGLSNIVEKAMGS
                     IIKSGNSAISGVLSPGEKVRQKGLIYAATPASDFICGTLQVAAGITLHVFTTGRGTPY
                     SLAEVPVIKVATRSDLARRWHDLMDIDAGSIATGAATIEDVGWELFRLMLDVASGRKE
                     TCAEKLKLHNALTLFNPAPVT"
     gene            1733..2575
                     /locus_tag="VHV99_25355"
     CDS             1733..2575
                     /locus_tag="VHV99_25355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3638748.1"
                     /translation="MTDSNLDRAAAQKPFRRLLLTGAAGNLGQQLRGALAAWADVVRI
                     SDIAPLGDVAAHEEASIVNLADEAAVHALLEGVDAVVHLGGISVEAPFEDLLEANIRG
                     LYNLYSAAQKQGVKRIVYASSNHVVGFHPTTSVLDIDAPPRPDTLYGVTKCFGESLSR
                     YYFDRFGLETVCLRIGSSFEQPKNPRMLVTYLSFRDFIELVRCSLFTNRVGHAIVYGV
                     SDNRARWVDNTKAAFLGFRPQDSSVEFEHLFPIAAPTAELDDPAQHFQGGAFILTGPM
                     EPKR"
     gene            2572..3513
                     /locus_tag="VHV99_25360"
     CDS             2572..3513
                     /locus_tag="VHV99_25360"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX3638749.1"
                     /translation="MSALALPPVERVEAAGQVPASVGESPVWRAAEQALYWVDIPARK
                     IVRLRLGSGERSEWVLPEKVACFAFDRRGTVLAGCETGLFAVTLTESTPKRTSSAEPQ
                     SVTGRKLAAPVFAFDDMRFNDGRCDRQGRFWSGTMVQDMAAANPAGALYRFDEHGVLS
                     QPVVDALITQNGLAWSPDGTTMYLSDSHPLRRQIWAFDYDTETGEPSNRRVFADLHDY
                     AGRPDGAAVDADGCYWICANDAGLLLRFTPQGKLDRQIAVPAVKPAMCAFGGNNLDTL
                     FVTSIRPAAGASEHDGHLFAVRPGVSGLPEPEYAGEL"
BASE COUNT          665 a         1124 c         1202 g          704 t
ORIGIN      
        1 cccatgttgt aggatgacgt atgaatggtc ggcgcaccaa gcctgaaacc gccataagga
       61 tcgatgtgat ggaacaaccc cccctctata ttcgcgttca ccccaacgac aacgtcgcga
      121 ttgttgtcaa cgacggcggt ctgggtgagg gcgccgtatt tcccgatggc ctcgccttgc
      181 gcgaacgtgt cccgcagggg cacaaggtcg cgctggccga tcttgcggaa ggcgacgagg
      241 tcgtccgcta taacgtggtg atcggttacg cactcaaggc cttgccgaag ggcagctgga
      301 tcaacgagca cgtcatccgc atgccgagtc cgcctggact ggaagatctg ccgatcgcga
      361 ccatcaagcc gccggcgatg ccgccgctgg aaggtttcac gttcgagggc taccgcaatg
      421 ccgacggttc ggtgggctcg cgcaacattc tggccattac gaccacggtg caatgcgtgg
      481 ccgatgtggt tcagcacgcg gtcacgcgca tcaaggccga actgctgccg aattatccca
      541 acgtcgacga cgtggtgagc ctcggccaca cctatggttg cggcgtcgcg atcgacgcgc
      601 cggacgcgat ggtgccgatc cgcacggtac gcaacatcag cctgaatccg aatttcggcg
      661 gcgaagtgat gatggtcagc ctcggttgcg agaagctgca gcctgaacgt ctgatgccgc
      721 cgggcacgat tccgattgcc gcggctgcga atgtcgccga agtcgccgac attggcgata
      781 tcgaggccga catgaacggc ggcgtggtgg tgcttcagga cgactcgcac gtcggcttcc
      841 agtcgatgat cgagtcgatc atgagaatgg ccgacggcca tctgaagcgg ttgaataacc
      901 gtcgtcgcga aacctgcccg gcctccgatc tggtgatcgg cgtgcaatgc ggcggcagcg
      961 acgcgttttc cgggctgacc gcgaatcccg cggtgggctt cgccaccgat ctgctggtgc
     1021 gcgccggcgc gacggtgatg ttctccgagg tcaccgaagt gcgcgacggc gtcgatcaac
     1081 tgacggcgcg cgcggccaac gcggatgtgg ctgcggcgat catccgcgag atgcagtggt
     1141 acgacgatta tctgaagcgc ggcggtgccg accgtagcgc caacaccacg cctggcaaca
     1201 agaagggcgg cttgtccaac atcgttgaaa aggcgatggg ctcgatcatc aagtcgggca
     1261 actcggcgat ctccggcgtg ctgtcgccgg gagaaaaggt caggcagaag ggcctgattt
     1321 acgcggccac accggccagc gatttcattt gcggcaccct gcaagtcgcg gcgggcatca
     1381 cgctgcatgt gttcaccacg ggccgcggca cgccgtacag tctcgcggaa gtgcccgtca
     1441 tcaaggtggc gacgcgctcc gatctcgcgc gccgctggca tgatctgatg gacatcgacg
     1501 ccggcagcat tgcaaccggc gcggcgacga tcgaagacgt gggttgggag ctgttccgcc
     1561 tgatgctcga cgtcgcaagc ggacgtaaag agacctgcgc ggagaaactc aaactccaca
     1621 acgcattgac gttgttcaat ccggcgccgg tgacctaata gtgctgcgag tgccggcagt
     1681 gctgattgtg gtgctgctgg tggctgaaat tcatcaagaa aagaactgag acatgactga
     1741 ctcgaacctt gaccgcgcag cggcacagaa gccctttcgc cgtcttctgc tcaccggcgc
     1801 tgccggtaat ctcggccagc agttgcgcgg tgcgctcgcc gcgtgggccg atgttgtgcg
     1861 catcagcgat atcgcgccgc tcggcgacgt ggcggcgcat gaagaagcct cgatcgtgaa
     1921 tctcgcggat gaagccgcgg ttcacgcgtt gcttgaaggc gtggatgcgg ttgttcatct
     1981 cggtggcata tccgtcgagg cgccgttcga agatctgctc gaggcgaata ttcgcggcct
     2041 gtacaacctc tattcggccg cgcagaagca gggcgtcaag cgcatcgtct acgcgagctc
     2101 gaatcatgtg gtgggtttcc atccgaccac gtcggtgctc gatatcgacg cgccgccgcg
     2161 tcccgacacc ctgtacggcg tgacaaagtg ctttggcgaa tcgctgtcgc gctattactt
     2221 cgaccgcttt ggtctcgaga cggtgtgcct gcgcatcggc tcgtcgttcg aacaaccgaa
     2281 gaatccgcgc atgctcgtca cctatctgag ctttcgcgac ttcatcgaac tggtgcgctg
     2341 ctcgctgttc accaaccggg tcgggcacgc gatcgtctat ggcgtgtcgg ataaccgggc
     2401 caggtgggtg gataacacca aggccgcgtt cctcggcttt cgtccgcagg acagttcggt
     2461 cgagttcgag cacctctttc ccatcgcggc gcctaccgcc gagctcgatg acccggccca
     2521 gcatttccag ggcggcgcct tcatactgac cgggccgatg gagccgaagc gatgagcgcg
     2581 ctggcgctcc cgcccgtgga gcgtgtagag gcggcggggc aggtgcccgc gtcggtcggc
     2641 gagagtccag tatggcgtgc ggcggaacag gcgctctact gggtggatat tccggcgcgg
     2701 aagatcgtgc gactgcggct cggttcggga gagcggtctg aatgggtgtt gccggagaaa
     2761 gtcgcttgct tcgcgttcga ccgccgcggc actgtgttgg cgggctgcga gacgggactt
     2821 tttgcggtca cgctaacgga aagcacgcct aagcgtacgt ccagtgccga accgcaatcc
     2881 gtgaccggca ggaagctcgc cgcgccggtc ttcgcgttcg acgatatgcg cttcaacgac
     2941 ggccgttgcg accgccaggg acgcttctgg tccggcacga tggtgcagga catggcggcg
     3001 gcgaatcctg ccggcgcgct ctatcgcttc gatgagcacg gcgtcttgtc gcagccggtc
     3061 gtcgacgcgt tgatcacgca aaacggtctg gcgtggtcgc ccgatgggac cacgatgtat
     3121 ctgtcggact cgcatcctct gcgcaggcaa atctgggcct tcgattacga caccgaaacg
     3181 ggcgagccga gcaatcggcg cgtgttcgct gatctgcatg attacgccgg gcgtcccgac
     3241 ggtgccgcgg tggacgcaga cggctgctac tggatctgcg cgaacgacgc gggactgttg
     3301 ctccgcttta cgccgcaagg caagctcgac cggcagattg ccgtgccggc cgtgaagccg
     3361 gcgatgtgcg cgttcggcgg caacaatctg gatacgttgt tcgtgacgtc gattcgtccc
     3421 gccgctggcg ctagcgaaca cgacggccat ctctttgctg ttcgccctgg cgtgagcggc
     3481 ctgcccgagc ccgaatacgc gggcgaactc taatcgataa gcacatcagg cgcgccgggg
     3541 tgggtcatac ccggcgcgat tcactccatg gccgtttgat tcatcactcg tcgtacaaca
     3601 cttctctgcc ttgtggtgta gggaccacat ttcaagacgc ttgtatccag gaaaaacagc
     3661 gcgtccggac acctgctcga ccacgcggcg tcgtt
//
LOCUS       DATASH010000952        17643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4909276,
            whole genome shotgun sequence.
ACCESSION   DATASH010000952 DATASH010000000
VERSION     DATASH010000952.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 17643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17643
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4909276"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(435..3242)
                     /locus_tag="VHV99_25365"
     CDS             complement(435..3242)
                     /locus_tag="VHV99_25365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3638750.1"
                     /translation="MPALCEICNARPAVARVTVVQNGERKTLSICDYDYRQLMRHQSM
                     VNPFDSLLGGGGLSRFFGGLGGAGADDEDGDEDAGFEAELPRESVDATDAFSEQTLEL
                     LQRAAEKAHELGRNELDTEHLLYVLADADVCAALLKELKLSPQDIKAYIDQHAQKGTA
                     SPDAPIEKMTVSPRLKKAFQYAFQASRDLGHSYVGPEHLLIGLAAVPDSIAGALLKKY
                     GVTPEALRQKVVKVVGKGAEDGRVDTPTGTPTLDKFGRDLTAMARQGKLDPVLGRAQE
                     IESTIEVLARRKKNNPVLIGEPGVGKTAIVEGLAQRIINGDVPEVLRGKRLVEVNINS
                     MVAGAKYRGEFEERAKQLIDEVTAKQDELILFIDELHTIVGAGQGGGEGGLDIANVLK
                     PALARGELSLIGATTLNEYQKYIEKDAALERRFQPVLVPEPTVEQTIVILRGLRDKLE
                     AHHQVTFADDAFVAAAELSDRYITSRFLPDKAIDLIDQAAARVRIGATSRPAAIQELE
                     AEIAQLKREQDYASSRKRFDEAKNFEARIGEKQAKLDELMEAWQRKTGSETLEVTVQS
                     IAEVVSRLTGIPVTELTQEERQKLLDMEKKLRERVVGQDDAVIAVSDAVRLSRAGLGQ
                     ANRPIATFLFLGPTGVGKTELAKALAETVFGDEQAVIRIDMSEYMERHAVARLIGAPP
                     GYVGYDEGGQLTERVRRRPYSVILLDEIEKAHPDVNNVLLQVFDDGRLTDGKGRVVDF
                     SNTIIIATSNLGASIIMDNLEKPEADRQAEKAIREELMGVLKGHFRPEFLNRIDEIIV
                     FHGLSRENIHSIVRIQLERVERTAAAQDITLKIDESVIDHLADAGYRPEFGARELKRQ
                     IRQELETRLAKEILGDRLKSGDTVDVSYDKASDEVKFSKSEAPPTPKATGGKGGNGRA
                     KKKPSKETRSPEPAESADAKK"
     gene            complement(3523..3942)
                     /locus_tag="VHV99_25370"
     CDS             complement(3523..3942)
                     /locus_tag="VHV99_25370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HEX3638751.1"
                     /translation="MLKSHVGHAEERIMSEQQGKLSDEFIAQQRQRLEALRRQLLGAE
                     ENTIANQRTAEEEHGDEAEEFEDEAQGMAQNEVNQALHDVNDQRISDIGRALEKIDEG
                     TYGLSDASGEPIPKARLESVPEAIFTVAEQSSREARK"
     gene            complement(3942..4139)
                     /locus_tag="VHV99_25375"
     CDS             complement(3942..4139)
                     /locus_tag="VHV99_25375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638752.1"
                     /translation="MQEFDFYINLKKPTLGLYVRTGAGLPDLVDISQWQFDGHVWQSE
                     LTPDILKGLEANGHAFQELGE"
     gene            complement(4184..4435)
                     /locus_tag="VHV99_25380"
     CDS             complement(4184..4435)
                     /locus_tag="VHV99_25380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638753.1"
                     /translation="MAQNEISYRRFVISATISPLQGNRGIVTIDVVTSDPERIADLGT
                     ERFLHVERWVERNDPAFLQVVVDECKVTIDHYANNVDDS"
     gene            4613..4996
                     /locus_tag="VHV99_25385"
     CDS             4613..4996
                     /locus_tag="VHV99_25385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6566 family protein"
                     /protein_id="HEX3638754.1"
                     /translation="MMVFRIAHRIVDGHPSNAAKEKTMPASTVSYGEFEIAVHPERNP
                     RGAWIARVNVTRGDRTVVSVRPETVQPEWLTEEEATRDGVEWGRRFIDREFNTPQSRS
                     WVADRAHAESWFRDVEEPPGSETSA"
     gene            complement(5020..5379)
                     /locus_tag="VHV99_25390"
     CDS             complement(5020..5379)
                     /locus_tag="VHV99_25390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HEX3638755.1"
                     /translation="MMTEVTWLVVADGGRGRVFQAPGLTLDLREIEDLINNEFTGTML
                     TEKDRLKFAKRVSDYLEEGRLHQRYDRLRLAMESKFLGMVKANLSEQTRRLIFEQLSE
                     DLSTLNTKEIEAHLRRH"
     gene            complement(5863..6870)
                     /gene="rhaS"
                     /locus_tag="VHV99_25395"
     CDS             complement(5863..6870)
                     /gene="rhaS"
                     /locus_tag="VHV99_25395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0033296 - rhamnose binding [Evidence IEA];
                     GO_process: GO:0015762 - rhamnose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnose ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX3638756.1"
                     /translation="MFNPLRHTGKAALCIALVALSCAASAAGLKSGLKIAFVPKQINN
                     PYEVIADDGGMAAIKEFGGVGKVVGPSDAGASSQVQYINTLITQRQDAIVIAANDANA
                     VVPYLKKAMSQGIKVVTFDSDTAPEGRQLFVNQANAEGIGRGQIQLVAKLMGGEGEFA
                     VLSATPNATNQNTWIKWMQEELKKPEYSKIKLVKIAYGNDDDQKSFVETQGLLQAYPN
                     LKAIVAPTTVGIAAAARYISSSSSKGKVQVTGLGTPNQMRAFVKNGTVKAFQLWDPNQ
                     LGYLAGYAAAALSSGTISGKEGESFDAGKLGKRTIGPQGEIILGPPTTFDSSNIDNFN
                     F"
     gene            complement(6973..7989)
                     /locus_tag="VHV99_25400"
     CDS             complement(6973..7989)
                     /locus_tag="VHV99_25400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3638757.1"
                     /translation="MAKPDSALLTRKRETPLQWEVLLVIVLALSLALGRWLSPVFLTS
                     ANVSNILADLTEIALMALPMTLIIVAAEIDLSVASVLGASSALMGVLWHMGLPMPVVI
                     VLVLIAGALAGLLNGLVIVKLNLPSLAVTIGTLALFRGLAYVLLGDQAVADFPAGYTA
                     FGMDTVGSTFIPLPFAIVIVGAVLFTVLLQSTAFGRSLYAIGANPTAAAFSGIEVAKI
                     RLRLFVLSSAMSALAGVVYTLRFTSARGDNGEGFELSVIAAVLFGGVSIFGGRGSMFG
                     VLLSLLIIGVLKNALTLDDVSSETLTIVTGVLLLASVLIPNLVARWRAARDRRFIAKS
                     ASSL"
     gene            complement(7982..9040)
                     /locus_tag="VHV99_25405"
     CDS             complement(7982..9040)
                     /locus_tag="VHV99_25405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018425424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3638758.1"
                     /translation="MMRHSPTHPAPVRAPVPKRTGSAPGGFVASIARSRETTLFVVLI
                     LLIAGTGLAKPQFLNLQNLRDVLLNVSIISLLTAGMTVVILMRHIDLSVGSTVGVSAY
                     AVGSLYVAFPHMPVIVALLAGLAIGLVAGGINALLVAVGRVPSLVATLSTLYIFRGAD
                     YAWVHGGQINATSLPDAFSRLATGTFFGIPTLALIAIVVLAALAVYLKQFRGGREHYA
                     IGSNPEAARLAGVNVERRVMAGFLLSGAIAGFAGALWLARFGTVDASTAKGIELQVVA
                     AAVVGSVAITGGVGTILGATLGALVLGVISIALVVLHVSPFWEQAIQGALIVAAITAD
                     TLLARSVAKRMMRKRDHG"
     gene            complement(9037..10566)
                     /locus_tag="VHV99_25410"
     CDS             complement(9037..10566)
                     /locus_tag="VHV99_25410"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEX3638759.1"
                     /translation="MQQSPTVAPRLELRHASKSFGRVRALSEGDLALWPGEVHALLGE
                     NGAGKSTVVKILAGVHQPDTGELLVDGVARRFATPAEARDAGLAVIYQEPTLFFDLSI
                     AENIFMGRQPVDRIGRIQYDAMRREVDGLLASLGVDLRADQLVRGLSIADQQVIEIAK
                     ALSLNANVLIMDEPTAALSLPEVERLFAIVRKLREREVAILFITHRLDEVFTLTQRVT
                     IMRDGAKVFDGLTADLTTEAIVAKMVGRDLETFYPKADRPPGEVRLSVRGLTRVGVFK
                     DISFDVRAGEIVALAGLVGAGRSEVARAIFGIDPLDSGEISIVGKRLTAGSPAAAVRA
                     GLALVPEDRRQQGLALELSIARNASMTVLGRLVKHGLISTRSETQLADQWGTRLRLKA
                     GDPNAPVGTLSGGNQQKVVLGKWLATNPKVLIIDEPTRGIDVGAKAEVYSALAELVRD
                     GMAVLMISSELPEVLGMADRVLVMHEGRISADIARAEADEERIMGAALGQPIPPLGHA
                     A"
     gene            complement(10686..11579)
                     /locus_tag="VHV99_25415"
     CDS             complement(10686..11579)
                     /locus_tag="VHV99_25415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018425426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3638760.1"
                     /translation="MQVVDSHIHLWDLKTHRYPWLENPGVSFVGDARELKHDYLLDDL
                     LGEAGDIDVLKLVHVEANHDPADPVEETRWLQSIADRPASRGRPNALVAAVDLSAPNA
                     PEILEAHASFANTRGIRQILNVHENKLFDYVGRHFMREPQWRGHFALLRRYDMSFDLQ
                     LYPSQMEEAAALARAHGETIFIINHAGMFVDRGSIAGYRAWRDGLRLLAGCRNVAVKI
                     SGLAMFDHQWTVESLRPYVLETIDTFGVERAMFASNFPVDRLFGSYADLWHAYASIVD
                     GASVAEKEALFCRNAERFYRI"
     gene            complement(11622..11945)
                     /gene="rhaM"
                     /locus_tag="VHV99_25420"
     CDS             complement(11622..11945)
                     /gene="rhaM"
                     /locus_tag="VHV99_25420"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HEX3638761.1"
                     /translation="METIAFRMVLNPGMREEYERRHAQIWPELVDALHNAGVRDYRIF
                     FDADSDHLFAILTRSTNHTMNQLPQLDVMRKWWDYMADIMQTAPDHTPLQQPLEPVFH
                     LNSLS"
     gene            12078..13076
                     /locus_tag="VHV99_25425"
     CDS             12078..13076
                     /locus_tag="VHV99_25425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3638762.1"
                     /translation="MSQHSAAIALVNRLKFKHLALLVALDDARNLHQAAEAINVAQPS
                     ASRMLGDIEEAFGFLLFERNARGMTPTPLGVVTLAYARRALAELTRFAEDLDVKRRGG
                     HGQLTVGAIMGAAPDLLAMAVAALKTESPLLNVRILGETSDQVVQLLHRREVDLALGR
                     LTSPLQHNDFSFEPLARETLLLVVRSVHPLAQRSRITLRELIDWPWVAQPVTSPARVL
                     FEEELARAGLATPVNLTECASIFATLQLLENYDAVAMLPESVVRDHVRGKLLVALPLE
                     IGKSLAGFGILTRKEEALAEPALRFIDLLRGFSRKLARDDSAVTEATPVIVSAPVH"
     gene            complement(13092..13874)
                     /locus_tag="VHV99_25430"
     CDS             complement(13092..13874)
                     /locus_tag="VHV99_25430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3638763.1"
                     /translation="MLLKDKVVIVTGGSRGIGRAIAVACATEGADVAINYWGDNDASY
                     GRRSAVAEVVGAIEALGRRVIAIEGNVAARETGHELVRQTVEAFGKVDVLASNAGICP
                     FHAFLDMPAEILESTVAVNLNGAFYVTQAAAQQMKAQGTGGAIVATSSISALVGGGMQ
                     THYTPTKAGVHSLMQSCAVALGPYGIRCNSVMPGTIATDLNAEDLADEAKKAYFEKRI
                     PLGRLGRPEDVADCVVFLASERARYVTGAALLVDGGLFVNLQ"
     gene            complement(13989..15167)
                     /gene="rhmD"
                     /locus_tag="VHV99_25435"
     CDS             complement(13989..15167)
                     /gene="rhmD"
                     /locus_tag="VHV99_25435"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HEX3638764.1"
                     /translation="MAMPTIRHVRAFIVRGGGADYHDQPGGHWIDDHISTPMARYPEY
                     RQSRQSFGINVLGTLVVEIEASDGTVGFAVTTGGEIGAFIVEKHLARFLEGQLVTDIE
                     KMWDQMYFSTLYYGRKGVVLNTISGVDLALWDLLAKVRKEPVYQLLGGPVRDELVFYA
                     TGARPDLAKEMGFIGGKLPLQHGPAEGEAGLRKNLEKLAEMRSRVGDDFWLMYDCWMS
                     LDVPYATRLAQAAHDYGLKWLEECLPPDDYWGYAELRRNVPRGMMVSTGEHEATRWGF
                     RMLLEMACCDLIQPDVGWCGGMTELIKISALADAHNVMVVPHGSSVYSYHFVVTRHNS
                     PFAEFLMMAPKADEVVPMFTPLLLDEPVPVNGRMKVPDTPGFGVRLNPECALVRPYPR
                     "
     gene            complement(15216..16544)
                     /locus_tag="VHV99_25440"
     CDS             complement(15216..16544)
                     /locus_tag="VHV99_25440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3638765.1"
                     /translation="MNPHSSTSLEAINSKVMRRLLPFLLLMYVLAFLDRANIGFAQKA
                     LQHDTGLSNAAFAFGAGVFFVGYALFEVPSNLLLHKVGARAWMCRIMVTWGLVSAAMC
                     LAHTPTAFYSLRFLLGVAEAGFFPGVIYYLTHWFPQAARARAVGVFYFGAPLAFIFGS
                     PLSGSLLELHGTMGLAGWQWLFLIEGTLASLVGVWAFWYLDNRPEDARWLDAEQRTLL
                     RSALDDDARAASSHGPHHILAALVDRRVLLLSAIYLLIQMSVYGVIFYLPQQVAALLG
                     TTVGLRVGLVAALPWLCALAVTWYVPRRADRTGGHRRWAVALLVLAGLGIGASGLAHN
                     PSIGLIALCCAASGFIAAQPLFWTFPTRYLTGAAAAGGIALINSLGGLGGFIAPSLRT
                     AAERAFSSTSAGLIVLGVSSLLAALMIGTLLRRDGTDSSWSFQHLLHRAR"
     gene            complement(16642..17394)
                     /locus_tag="VHV99_25445"
     CDS             complement(16642..17394)
                     /locus_tag="VHV99_25445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3638766.1"
                     /translation="MNRIDLEGRVVAITGGARGIGYAVAQRALNSGASVALWDIDAER
                     LARSVSELSGLGKVTAVTVELTQEASVDQAVAQTVAAHGALDVLINCAGITGGNGTTW
                     ELDPEVWRRVIDVNLIGPYLTCRAVVPQMLKQGYGRIVNIASVAGKEGNPNASHYSAS
                     KAGLIGLTKSLGKELATKNILVNAVTPAAAKTEIFDSMSQQHIDYMLSKIPMNRFLLP
                     EEAASLILWLSSEDCAFSTGAVFDLSGGRATY"
BASE COUNT         3444 a         5635 c         5147 g         3417 t
ORIGIN      
        1 cgaagatttc catgatcgat caacggctcc gagtggcaac ggcatgtgcg taaagacggg
       61 caccatgtgg cgacggaacg gctacgcggc acatcgtagc gcgataacgt gaggtctgca
      121 taaaaacggg gggaatcagg aatcgcgcgt atgtaagaga catgcgggtg cgcgattaac
      181 gccaacccgg attcggggtg ccgcgagcct tcgtggcgtg tctccatcgt gcacgcgtga
      241 ttcatcccgg agtctggccg atcgggaaga tgagtctggt ctcaccgaag ttgatgcacg
      301 gcgaaaggta caggcaaaca gcggcccacg tggacagtgc aagtacagtg agaccgccgt
      361 ctgtttcaac cggtcttcag aggccgcacg tcgtcagcga tgacgctgcg gcttctccca
      421 gcttgctggc tacgtcattt cttcgcgtcg gcgctttcag ctggctcggg gctgcgggtt
      481 tctttagacg gttttttctt cgcgcgtccg ttgccgccct ttccgcctgt cgccttgggt
      541 gtaggcggcg cctcgctctt gctgaacttc acttcgtcgc ttgccttgtc gtaactgacg
      601 tcgacggtat cgcccgactt cagccggtca ccgagaatct ccttcgcgag ccgcgtttcc
      661 agttcctggc gaatctggcg cttcaactcg cgtgccccga attccggccg atagccggca
      721 tcggcgagat gatcgatcac ggattcgtct atcttgaggg taatgtcctg ggcggccgcc
      781 gtgcgctcca cacgttcgag ctggatccgc acgatcgaat ggatgttctc acgcgacagc
      841 ccatggaaga cgatgatctc gtcgatccgg ttcaggaatt cagggcggaa gtgccccttg
      901 agcacgccca tcaattcctc gcggatcgct ttttcagcct ggcgatccgc ttcgggtttc
      961 tcgaggttgt ccatgatgat cgatgcgccg agattgctcg tcgcaatgat gatcgtgttg
     1021 ctgaaatcca ctacacgtcc cttgccgtcg gtcagccgcc cgtcgtcgaa cacctgcagc
     1081 agcacgttgt tcacgtcggg gtgggccttc tcgatctcat cgagcaggat cacgctgtac
     1141 gggcggcgcc ggacgcgctc ggtcaactga ccgccttcgt cgtaaccgac gtagcccggc
     1201 ggggcgccga tcagccgtgc aaccgcgtga cgctccatgt attcggacat gtcgatacgg
     1261 atgaccgcct gctcgtcgcc gaacaccgtt tccgccagcg ccttcgcgag ttcggtcttg
     1321 ccgacgccgg tgggtccgag aaacaggaac gttgcaatcg gccggttcgc ctggcccagg
     1381 cccgcacggg acaggcgcac ggcatcgctc acggcgatca ccgcgtcgtc ctggccgacc
     1441 acgcgctcgc gcagcttctt ctccatatcc agcagtttct gccgctcttc ctgcgtgagt
     1501 tccgtgacgg gaatgccggt gagacgcgac acgacttcgg cgatggactg cacggtcacc
     1561 tcgagtgttt ccgagccggt cttgcgctgc caagcctcca tcagctcgtc gagtttcgcc
     1621 tgcttctcgc caatccgcgc ctcgaaattc ttcgcctcat cgaagcgctt gcgcgaggac
     1681 gcatagtcct gctcacgctt gagctgggcg atttcggctt cgagctcctg aatggccgcc
     1741 ggtcgcgagg tcgcgccgat ccgcacgcgt gccgccgcct ggtcgatcag gtcgatggcc
     1801 ttgtccggca aaaaacgcga cgtgatgtag cgatcggaaa gttcggcggc ggcgacgaac
     1861 gcatcgtcgg caaacgtcac ctgatggtgc gcctcgagct tatcgcgaag cccacgcaga
     1921 atgacgatgg tctgctccac cgtgggttcg ggtaccaaca ccggttggaa gcgccgttcg
     1981 agcgcggcgt ctttttcgat gtacttctga tattcgttga gtgtcgtggc gccaatcaga
     2041 ctgagttcgc cacgcgcaag cgcgggcttg agcacgttcg cgatatcgag gccgccttcg
     2101 ccaccgcctt gtcccgcgcc gacgatcgtg tgcagttcgt cgatgaacag gatcaattcg
     2161 tcctgcttcg ccgtgacctc atcgatcagc tgcttggcgc gctcctcgaa ttcgccgcga
     2221 tacttcgcgc cggccaccat cgagttgatg ttgacctcga caagccgctt gccgcgcaga
     2281 acctcgggca cgtcgccgtt gatgatgcgc tgtgccagcc cttcgacgat cgcggtcttg
     2341 ccgacacccg gctcgccgat cagtacaggg ttgttcttct tgcgccgcgc gagcacttcg
     2401 atcgtgctct cgatttcctg cgcgcggccg agcaccggat cgagcttgcc ctgacgcgcc
     2461 atggccgtga gatcacgccc gaatttgtcg agcgtgggtg tgccggttgg cgtgtcgaca
     2521 cggccgtcct ccgcgccttt gccgacgact ttcacgacct tctgccgcaa cgcctccggc
     2581 gtcacgccat acttcttcag cagtgcgccc gcgatgctgt ccggcaccgc cgcgaggccg
     2641 atgagcagat gctccgggcc aacataggaa tgccccagat cacgcgacgc ctggaacgcg
     2701 tattggaacg cctttttcag gcgcggcgaa accgtcatct tctcgatcgg cgcgtcaggt
     2761 gaggcggtgc ccttttgcgc atgctggtcg atataagcct tgatgtcctg cggtgagagc
     2821 ttgagctcct tcagcagcgc ggcgcacacg tcggcgtccg cgagcacgta aagcaggtgc
     2881 tcagtgtcga gttcgttgcg gccgagctcg tgtgcttttt ccgcggcgcg ttgcaacaac
     2941 tcgagtgttt gctcgctgaa agcatcggtt gcatcgaccg actcacgtgg caattcggcc
     3001 tcgaaacccg cgtcttcgtc tccatcttcg tcatcggctc ctgcgcctcc caggccgccg
     3061 aagaagcgcg acaagccgcc tcctccgagc agtgaatcga atgggttgac catgctttgg
     3121 tgccgcatca attgccgata gtcgtagtcg caaatcgata aggtcttccg ctcgccgttt
     3181 tgcacaacgg tcacacgagc aacagcggga cgggcattac agatttcgca gagggcgggc
     3241 atcgagagtc acctttttcg gttaaggcat ccggctataa caggagaacc ctgatagcgt
     3301 ggcgcggcgg gcgcggtata tgcatggacg tcgagcgaac tttgtctgac acatgcgggg
     3361 cgcgtgcggg gatcgatctc agcatgttta gcatgccgaa gccccaaatc cgcccttgga
     3421 ttctatgagc ggtctgtcga taaagaaata tttgtttttc cgatggtcga aggcgattca
     3481 atacgcgccg ctacacattg gaagccgtca ttttgcaagc cgtcatttgc gggcctcacg
     3541 gctactctgc tctgcaacgg tgaaaatagc ttccgggacg gattcgagcc gtgctttcgg
     3601 tatcggctcg ccactggcat cggagagacc gtaagtcccc tcgtcgatct tctcaagcgc
     3661 gcgcccgata tcgctgatgc gctggtcgtt cacgtcatgg agtgcctgat tgacctcgtt
     3721 ctgcgccatt ccctgcgcct catcctcgaa ctcttcggcc tcgtcgccgt gctcttcttc
     3781 ggccgtccgc tgattcgcga tcgtgttttc ctctgcgcct agcagttgcc ggcgcaacgc
     3841 ctcgaggcgc tggcgttgct gcgcgatgaa ctcatcactc aatttgccct gttgctccga
     3901 catgattctc tcctcggcat ggccgacatg tgatttcaac atcactcacc caactcctga
     3961 aatgcgtggc cgttggcctc gagccccttc aggatgtcgg gtgtcagctc gctttgccac
     4021 acgtgtccgt cgaattgcca ctggcttatg tcaacgaggt cgggaagtcc ggcgcccgtg
     4081 cgcacataga gtccgagtgt cggcttcttc aggttgatgt agaaatcgaa ttcctgcatt
     4141 tcaatacctc atgcaaggta gcgtttgcgg ccagacgcaa gaatcaacta tcgtcgacgt
     4201 tgtttgcgta atggtcgata gttactttac attcgtccac aacgacctga agaaaggcag
     4261 gatcatttct ttcaacccac cgctcaacgt ggaggaagcg ttccgtgccg agatcggcaa
     4321 tacgctccgg atccgaggtc acgacgtcga tcgtcacgat cccccggtta ccctgcagag
     4381 gggagatcgt cgcggaaatg acaaaccgcc ggtatgagat ttcattctgt gccatgcagc
     4441 ctcccctcaa tcgagaatat ggcgcctcgc gcgcccgcca cacaggaagt ctacgagcaa
     4501 gtcacgacga tgtgaacgcc ggttttctcc gatgccagat atgggtgaaa aacgcctatc
     4561 gggttgccat tgcgccttga tatccgcact cccggaatgc tggaattgcg gcatgatggt
     4621 gttcaggatc gcgcatcgga ttgtggacgg acacccatcg aacgctgcaa aggagaaaac
     4681 gatgccagca agcactgttt cttacggcga gtttgagatc gccgtgcacc ctgagcgcaa
     4741 cccgcgcggc gcctggatcg caagggtgaa cgtgactcgc ggcgatcgca cggttgtcag
     4801 tgttcgcccc gagaccgttc aacccgagtg gctcactgaa gaagaagcga cgagagacgg
     4861 ggtcgaatgg gggcgccggt tcatcgaccg tgagttcaac acgccgcagt cccgctcctg
     4921 ggttgccgac cgcgctcacg cagaaagctg gtttcgcgat gtagaagaac cgcctggttc
     4981 cgaaaccagc gcataagtct tgcgaccgaa ggcggccagt cagtgccgcc gcagatgggc
     5041 ctcgatttct ttcgtattaa gtgtcgacag atcctcgctt agttgctcga atatcaatcg
     5101 acgggtctgc tcgctcagat ttgctttgac catgccgaga aatttcgact ccatggcaag
     5161 tctcagccga tcgtagcgct gatggagccg cccttcttcc agataatcgg atacccgctt
     5221 tgcaaatttt agccggtcct tttccgtcag catcgtgccg gtaaattcat tgttgatgag
     5281 atcttcgatt tcccgcaagt cgagtgtgag tcctggcgcc tggaaaacac gcccgcggcc
     5341 tccgtcggcc acaacaagcc aggtaacttc ggtcatcatg cacctcttgg caggaattcc
     5401 gctcgttcgg cgctcgccgc gacgggtcgc tttcgttctc gccgaagacc gcgaccctcg
     5461 actgttgtgt caacgaccat ccgctgtccg aacaactctt actcaagata gcagttcggg
     5521 ccaactacgc ccaaggtcaa tttgccaatt tatcgtatcg gtccaaagtt cgtgtggaag
     5581 agccgcgttc gcgtatcgca agcaattccg gatcgggtcg cttcgtatgc atatcctgta
     5641 cctgcattct ggcggatgga accggtcagc ccaggatcgg gctcgtcgca cgatctgcgg
     5701 cggcgcgatt gagaaacgag cggccccgtc gtcgattgac cagacggggc cgctgtagtc
     5761 gaggtacacc gaaatgtcca aaggcttgcg atacgaaagc ttctgcctcg cgccgcgtac
     5821 ttggcgtagc aatgcccggc gtcgcggcgc ccggctcgct gcctagaaat tgaagttgtc
     5881 gatattgctc gaatcgaagg tggtcggcgg tccgagaatg atttcgcctt gcgggccgat
     5941 cgtgcgctta cccagtttgc cggcatcaaa cgactcgcct tccttgccgc tgatcgtccc
     6001 cgacgacagc gccgcagcgg cataacctgc cagatagcca agctgattcg gatcccaaag
     6061 ctggaacgcc ttaaccgtcc cgttcttgac gaacgcacgc atctggttcg gcgtgcccaa
     6121 gccggtcacc tgcaccttgc ccttgctcga tgacgacgag atgtaacgcg ccgccgccgc
     6181 gatgccgacc gtagtcggcg cgacgatcgc cttcagattc ggatacgcct gcagcaagcc
     6241 ttgtgtttcg acgaacgact tctggtcgtc gtcgttgccg tacgcgatct tcacgagctt
     6301 gatcttcgag tactcgggct ttttcaactc ctcctgcatc cacttgatcc acgtgttctg
     6361 attggttgca ttaggcgtgg ccgacagcac cgcgaattcg ccctcgccgc ccatcagttt
     6421 ggccaccagc tggatctgcc cccggccgat cccttccgca ttcgcctggt tgacgaacaa
     6481 ttgacggcct tcgggcgctg tgtccgagtc gaaggtcacg accttgatgc cctgcgacat
     6541 cgctttcttc agatacggca cgacggcgtt cgcgtcgttg gccgcgatca cgatcgcgtc
     6601 ctgccgctgc gtgatcaacg tgttgatgta ttgcacctgc gacgacgcgc cggcatccga
     6661 gggccccacg accttgccca cgccgccgaa ttccttgatc gctgccatgc cgccgtcgtc
     6721 ggcaatcact tcataagggt tgttgatctg cttcggcacg aaagcaatct tcagaccgct
     6781 tttcagcccc gctgccgacg cggcacagct gagcgcgacg agagcaatgc acagcgcagc
     6841 tttgccggtg tgacgtagag gattgaacat aaggcgtctc ctgctttgtt gttggacgaa
     6901 aacctgtccg ggttgtaggt atcgggtatt cgatgtctgg tatttcttct ggcgccggtt
     6961 ttaagaagaa cgttataaag aagaagcgga cttcgcgatg aaacgccggt cacgcgccgc
     7021 ccgccagcgg gcaaccagat tgggaatcag caccgatgcc agcagcagga cgcccgtcac
     7081 gatcgtcagc gtttcgctcg atacatcatc tagcgtcagc gcgtttttca acacgccgat
     7141 aatcagcagc gacaacagga caccgaacat cgaaccacgt ccaccgaaaa tactcacgcc
     7201 gccgaacagc accgccgcga tcaccgacaa ttcgaaacct tcgccgttat cgccgcgcgc
     7261 gctggtgaag cgcagcgtat agacgacgcc ggccagcgcg ctcatcgcgc tggacaacac
     7321 gaacagccgc agccggatct tcgcgacttc gatgccggag aacgccgcgg cggtgggatt
     7381 cgcgccgatt gcgtagaggc tgcggccgaa tgcggtggac tgcagcaaca ccgtaaacag
     7441 caccgccccg acgatcacga tcgcaaacgg caacggaatg aaggtcgagc caaccgtatc
     7501 catgccgaac gccgtatacc ccgccggaaa atccgcgacc gcctgatcgc cgagcagcac
     7561 atacgcgaga ccgcgaaaca gggccagcgt accgatcgtg accgccagcg aaggcagatt
     7621 gagcttcacg atcacgaggc cgttcagcaa gcccgccaac gcaccggcaa tcaataccag
     7681 cacgatcacc accggcatcg gcaagcccat atgccacagc acgcccatca gcgcactgga
     7741 cgcgcccagc accgacgcca ccgaaagatc gatttccgcg gcgacgataa tcagcgtcat
     7801 gggcaacgcc atcaacgcga tctcggtcag atcggcgagg atgttgctga cattcgcact
     7861 ggtgagaaac accggcgaca accagcgccc gagggcgagc gatagcgcca gcacgatgac
     7921 cagcaacact tcccattgca acggcgtttc gcgtttgcgc gtgagcaacg cggaatcggg
     7981 tttagccatg atcgcgcttc ctcatcatgc gtttggcgac ggagcgggcc agcaaggtat
     8041 cggcggtaat cgcggcgacg atcagcgcgc cctggatcgc ctgctcccag aatggcgaga
     8101 cgtgcagcac gaccagcgcg atgctgatca cccccagtac gagcgcgccg agcgtggcgc
     8161 cgagaatggt gcctacccca cccgtgatcg cgacactgcc caccacggcg gcggccacta
     8221 cctgcaattc gatgcccttt gccgtgcttg catcgacggt gccgaagcgc gcgagccaca
     8281 atgcgcccgc aaagccggca atcgcgccgg acagcaaaaa gcccgccatc acgcgacgtt
     8341 cgacgttcac gccggcaagc cgtgccgcct ccgggttcga gccgatcgcg tagtgttcgc
     8401 ggccgccgcg aaactgcttc agatagacag ccaacgctgc cagcacgacg atcgcgatga
     8461 gcgcgagcgt cggtatgccg aagaatgtac cggtggcgag acgggaaaac gcatcgggca
     8521 aactggttgc attgatctgc ccgccgtgga cccacgcgta gtccgcgccg cgaaagatgt
     8581 agagcgtgga gagcgtggcc accagcgacg gcacgcgtcc cactgcgacc agcagcgcat
     8641 tgatcccgcc cgccaccaga ccgatcgcaa gtccggccag caacgcgacg atcaccggca
     8701 tatgcggaaa cgccacgtac aggctgccga ccgcataggc gctcacgccg accgtcgatc
     8761 ccaccgacag atcgatatgc cgcatcagga taacgaccgt cataccggct gtcagcaagc
     8821 tgataatcga cacattgagc agcacgtcac gcagattctg cagattcaga aactgcggtt
     8881 ttgcgagccc ggtgcccgcg atcaacagga tcagcacgac gaaaagcgtc gtctcgcggc
     8941 tcctggcaat gctcgcgacg aagccgcccg gcgcgctgcc ggtacgcttg gggacgggtg
     9001 cccgaaccgg cgcgggatga gttggcgaat ggcgcatcat gcggcgtgtc ccaacggggg
     9061 aatcggttgg ccgagcgccg cgcccatgat gcgttcttcg tcggcttcag cgcgcgctat
     9121 atcggcgcta atacggccct cgtgcatcac cagcacgcga tcggccatgc cgagtacttc
     9181 gggcagttcg ctcgaaatca tcagcacggc catgccgtcg cgcaccagct ccgccaacgc
     9241 gctatacacc tccgctttgg cgccgacgtc gataccgcgc gtgggttcgt cgatgatcag
     9301 gaccttcggg ttagtggcaa gccacttgcc cagcacgacc ttttgctgat tgccgccgga
     9361 gagcgtgccg accggcgcat tcggatcgcc cgctttgaga cgcaagcggg tgccccactg
     9421 atcggccagt tgcgtttcgc tgcgggtgga aatcaggcca tgtttgacga gccgtccgag
     9481 cacggtcatc gaggcattgc gcgcaatgct cagctccaat gccagccctt gctggcgacg
     9541 gtcttccggt accagcgcga ggccggcacg caccgccgcg gccggactgc ccgcggtgag
     9601 acgcttgcca acaatggaaa tctcgcccga gtcgagggga tcgatgccga agatcgcgcg
     9661 ggccacttcg ctgcggcccg cacccacgag gcccgccaac gcgacgatct cgccggcgcg
     9721 gacgtcgaag gaaatgtcct tgaagacgcc tacgcgcgtg agtccacgca ctgacaggcg
     9781 cacttcaccc ggcggccggt cggccttggg atagaacgtt tccagatcac ggcccaccat
     9841 cttcgccacg atggcttcgg tggtgagatc ggcggtgagg ccgtcgaata ctttcgcacc
     9901 gtcgcgcatg atcgttacgc gttgcgttag cgtgaagact tcatcgagcc ggtgcgtaat
     9961 gaacagaatc gccacctcgc gctcgcgcag cttgcgtacg atcgcaaata gccgctccac
    10021 ttcgggcagc gacaaggcgg cggtcggctc gtccatgatc agcacgttcg cgttcaacga
    10081 caacgccttg gcaatttcga tcacctgctg atcggcgatc gacagaccac gcaccaattg
    10141 atccgcgcgc agatcgacgc cgagcgaggc cagcaagcca tccacctcgc ggcgcatggc
    10201 gtcgtattga atacggccga tccggtcgac tggttgccgt cccatgaaga tgttctccgc
    10261 gatcgagaga tcgaagaaca gcgtcggctc ctgatagatc accgcgaggc ccgcgtcacg
    10321 cgcttccgcg ggcgtggcga aacggcgcgc cacgccgtcg accagcagct cgccggtatc
    10381 cggctgatgc acgcccgcga gaatcttgac gaccgtcgat ttgcccgcgc cgttttcgcc
    10441 gagcagcgca tgcacttcgc ccggccacaa cgcgagatcg ccctcggata acgcgcggac
    10501 ccggccgaag gatttgctgg cgtgccggag ttcgagcctg ggcgccacgg ttggggattg
    10561 ctgcactacg tgtctccttc ctgttcgctt tcgagtgtgc gtcagatgcg ctgtgtcggg
    10621 tgaactgagt caggtccgct gaatcaagtc cgccgggtca ggacgtttga gtcaggtccg
    10681 ccaggtcaga tacgataaaa gcgctccgcg ttacggcaaa acaacgcttc tttttcagca
    10741 acgctcgcgc catccacaat cgacgcatac gcgtgccaca aatccgcata agagccgaac
    10801 aaccggtcga ccggaaagtt agacgcgaac atcgcgcgct ccacgccgaa ggtgtcgatc
    10861 gtttcaagca cataaggccg caggctttcg accgtccact gatgatcgaa catcgcgagt
    10921 ccgctgatct tcaccgcgac gttcctgcaa cccgcgagca gtcgcaaccc gtcacgccac
    10981 gcgcgataac cggcaatgct gccgcggtcc acgaacatgc ccgcatggtt gatgataaaa
    11041 atcgtctcgc catgcgcgcg cgccaacgca gccgcttctt ccatctgcga cggatacagt
    11101 tgcaggtcga acgacatgtc gtaacggcgc agcaacgcaa aatggccgcg ccactgcggc
    11161 tcgcgcatga aatggcggcc gacgtaatcg aacagcttgt tctcgtgcac gttcaggatc
    11221 tgcctgatgc cacgcgtatt ggcgaacgac gcatgcgctt cgagtatttc aggtgcgttc
    11281 ggcgcggaca gatccacggc ggcgacaagc gcattcggcc taccgcgcga cgccggacga
    11341 tccgcgatgg attgcagcca gcgggtttcc tcgaccggat cggcgggatc gtggttcgct
    11401 tcgacgtgca ccagtttcag cacgtcgata tcgccggcct caccgagcag gtcgtcgagc
    11461 agatagtcgt gcttgagctc gcgcgcgtcc cccacgaacg acacacccgg gttttccagc
    11521 cacggatagc gatgcgtctt cagatcccac agatggatat gcgaatcgac aacctgcatg
    11581 cgacaccctc cgcccataag gcccaagcaa agtctgcaac gtcagctaag tgaattcaga
    11641 tgaaagaccg gctcgagtgg ttgctgaagc ggtgtatggt caggcgctgt ctgcatgatg
    11701 tcggccatgt aatcccacca tttgcgcatc acgtcgagtt gcggcagctg attcatggtg
    11761 tgattcgtgc tgcgcgtcag aatggcgaaa aggtgatccg agtccgcgtc gaagaagatc
    11821 cgatagtcgc gcacaccggc gttgtgcaac gcatcaacca gctcgggcca gatttgcgca
    11881 tgacgtcgtt cgtattcctc gcgcatgccg gggttgagca ccatccggaa agcgattgtt
    11941 tccattgcgg cctgtctccc gtcgtatccg ggcgctttct atgagaagcg actgatacga
    12001 ctataattcc tgcgtcgata gcatctcaat ccgttgttgg gattgggcga tccacaaacc
    12061 gaatcgcacc cgcaaccatg agccaacact cagctgccat cgcactcgtc aatcggctca
    12121 agttcaagca cctcgcactg ctcgtcgcgc tcgacgacgc gcgcaatctc catcaagccg
    12181 ccgaggcgat caacgtcgcg caacccagcg cgagccgcat gctcggcgac atcgaggaag
    12241 cgttcggctt cctgctgttc gagcgcaacg cgcgcggcat gacgcccacg ccgctcggcg
    12301 tggtcacgct ggcgtatgcg cggcgggcgt tggccgagtt gacccgcttt gccgaagacc
    12361 tcgacgtcaa acgccgcggc gggcatggac aattgaccgt cggcgccatc atgggcgccg
    12421 cgcccgatct gctggccatg gcggtagccg ccctcaaaac cgaaagcccc ttgctgaacg
    12481 tgcgcattct tggcgaaacc agcgaccagg tcgtgcaatt gctgcatcgc cgcgaagtcg
    12541 atctggcctt gggacgcctg accagtccgt tgcaacacaa cgatttcagt ttcgagccgc
    12601 tcgcccgcga gacgttgctg ctggtggtgc gttcggtgca tccgttggca cagcgctcgc
    12661 gcatcacgtt gcgggagctg atcgactggc cgtgggtggc gcaaccggta acgagcccgg
    12721 cgcgcgtgct gttcgaagag gaactcgcgc gtgccgggct cgcgacgcca gtcaatctga
    12781 ccgagtgcgc gtcgatcttc gccacgctgc aattgctcga gaactacgac gccgtggcca
    12841 tgctgcccga gtcggtggtg cgcgatcatg tgcgcggcaa actgctggtc gcgttgccac
    12901 tcgaaatcgg caagagcctc gccggtttcg gcattctcac gcgcaaggaa gaagcccttg
    12961 ccgaaccggc gctgcgcttt atcgatttgc tgcgcggctt ctcgcgcaag ctcgcacgcg
    13021 acgattccgc cgtgactgag gccacgcctg tgatcgtctc cgcaccggtt cattgacgtc
    13081 ggcatcgcgc gttactgcaa gttgacgaac aggccgccgt ccactagcag cgcggcgcct
    13141 gttacgtaac gcgcgcgctc agaagcgagg aataccacgc agtcggccac atcctccggg
    13201 cgacccagtc tgcccaatgg aatgcgcttt tcgaagtagg cttttttggc ttcgtcggcg
    13261 agatcttctg cgttcagatc ggtggcgatc gtgccgggca tcactgaatt gcagcgaatg
    13321 ccgtatggcc ccaatgccac cgcgcaggac tgcatcaacg aatgcacgcc cgccttggtc
    13381 ggcgtgtagt gggtttgcat gccgccgcct accagcgcgc tgatcgaact cgtcgccacg
    13441 atcgcgccgc ccgtgccctg cgctttcatc tgctgcgcgg cggcttgcgt gacgtagaac
    13501 gcgccgttca gattgactgc caccgtcgac tccaatattt cagcgggcat gtcgagaaag
    13561 gcatggaatg ggcagatgcc ggcattgctc gcgagcacgt ccactttgcc gaaggcctcg
    13621 acggtctggc gaacgagttc gtgaccggtt tcgcgcgcgg ctacgttgcc ttcgatcgcg
    13681 atcacacgcc gccccagcgc ttcgattgcg ccgaccactt ccgcaacggc cgaacggcgc
    13741 ccgtaagatg cgtcgttatc gccccagtag ttgatcgcca catccgcgcc ttcggttgcg
    13801 cacgcaaccg ctatcgcgcg gccaatgcca cgcgagccgc cggtgacgat cacgaccttg
    13861 tccttgagca acacgtcatt ctcctgttgg ctgggtcaat acccggtctt gcaggttggg
    13921 cctgagttca ccgcgcattc atcaaagcgc attcatcaaa acgcattcac cacgtcttca
    13981 ccgcgcaatc atcgcggata agggcgtacc agcgcgcatt ccggattcaa tcgcacaccg
    14041 aagcccggcg tgtccggcac tttcatccgt ccgttcacag ggaccggttc atcgagcagc
    14101 agcggcgtaa acatcggcac cacttcatcg gccttcggcg ccatcatcag aaactcggca
    14161 aacggcgagt tgtgccgcgt gacgacgaag tgatagctat acaccgatga cccgtgcggc
    14221 accaccatca cgttgtgcgc atcggcgagc gcggagatct tgatgagttc ggtcatgccg
    14281 ccgcaccagc ccacatccgg ctgaatcaga tcgcagcacg ccatctccag cagcatgcga
    14341 aagccccagc gtgtcgcttc atgctcgccg gtcgacacca tcatgccgcg tggcacgttg
    14401 cggcgcagtt cggcgtaacc ccagtagtcg tccggcggca gacattcttc gagccacttc
    14461 agaccgtagt cgtgcgccgc ctgcgcgagc cgcgtggcat acggcacgtc aaggctcatc
    14521 cagcagtcgt acatcagcca gaagtcgtcg ccgacacggc tacgcatctc ggcgagcttc
    14581 tccaggtttt tcctgagacc ggcctcgcct tccgcgggac catgctgcaa cggcaattta
    14641 ccgccgatga aacccatctc tttcgccaga tccggacgcg cgccagtcgc gtagaacacg
    14701 agttcgtcgc gcaccgggcc gcccaatagt tggtacaccg gctccttgcg gaccttcgcg
    14761 agcaaatccc acaacgcgag atcgacgccc gagatcgtat tcagcacgac gccttttcgg
    14821 ccgtaataca gtgtcgagaa atacatctga tcccacatct tctcgatgtc ggtaacaagc
    14881 tggccttcga gaaagcgcgc gagatgtttt tcgacgatga atgcgccgat ctcaccgccc
    14941 gtcgtgaccg cgaaaccgac tgtgccgtcg ctcgcttcaa tttcgacgac cagcgtgccg
    15001 agcacattga taccgaacga ctggcggctc tggcgatact ccggatagcg cgccatcggc
    15061 gtggaaatgt gatcgtcgat ccagtgtccg ccgggttgat cgtgatagtc cgcaccgccg
    15121 ccgcggacga taaaggcacg cacgtgccgg atagtaggca tggccatgaa aagggctcca
    15181 ttgttggtgc gcagaacgtg cagccgaaaa tgcgcctagc gggcgcggtg cagtaagtgt
    15241 tggaaagacc agcttgaatc tgtaccgtca cggcgcaaca gcgtgccgat catcaacgcc
    15301 gccagcagac tcgacacgcc caatacgatc agccctgccg aggtcgacga aaacgcccgt
    15361 tccgccgcgg tgcgcaggct tggcgcaatg aatccgccga gaccgccgag cgaattgatc
    15421 aacgcgatcc cgcctgccgc cgcggccccc gtgaggtagc gcgtcggaaa tgtccagaac
    15481 agcggttgcg ccgcgatgaa accactggcc gcgcagcaca gcgcaatcaa accgatcgac
    15541 gggttatgcg cgagccccga ggcgccaatg ccaaggcctg ccagcaccag cagcgccacc
    15601 gcccagcgcc ggtgaccgcc cgtgcgatcc gcacgacgcg gcacgtacca cgtcacggcc
    15661 aacgcacaca gccagggcaa cgcggccacg aggcccacgc gcaagccgac ggtcgtgccg
    15721 agtaaagcgg ccacttgctg cggcaaatag aagatcacgc cgtagacgct catctgaatc
    15781 agcagataga tcgccgagag cagcaacaca cggcggtcca ccagcgcggc gagaatatga
    15841 tgcggtccat gagaggaggc ggcgcgcgca tcgtcgtcca gtgcgctgcg caacagggtg
    15901 cgctgctctg cgtcgagcca gcgcgcgtct tcagggcggt tgtcgagata ccagaacgcc
    15961 cataccccga ctaacgaggc caacgtgccc tcgatcagga atagccactg ccagccggcc
    16021 aaacccatcg tgccgtgcaa ttcgagcagg gaacccgaca gcggactgcc gaagatgaac
    16081 gccagcggcg cgccgaaata aaacacaccc accgcgcgcg ctcgcgccgc ttgtggaaac
    16141 caatgggtca ggtagtagat cacgccgggg aagaaacctg cttcggcgac accaagcaga
    16201 aaacgcagtg agtagaacgc cgtcggcgta tgcgcaagac acatcgccgc cgagacgagc
    16261 ccccatgtca ccatgatccg gcacatccat gcacgcgcgc cgaccttatg cagtagaaga
    16321 ttgctcggta cttcgaacaa tgcataaccg acgaagaaca cgcccgcacc gaaggcaaaa
    16381 gccgcattgg acaaacccgt atcgtgctgc aacgccttct gcgcgaagcc gatattggca
    16441 cgatcgagaa acgccagcac gtacatcagc agcaggaacg gcaacagccg gcgcatgacc
    16501 ttgctgttga tcgcctccag tgacgtggac gaatgcggat tcatgtcgcc tccttcgcag
    16561 agcatgcggg tcgcgctccg cgtttttttt ccttctgcat ggctactgct accgccacgg
    16621 tccaggtgac acggtgacga ctcagtaagt agcgcgaccg cctgacagat cgaacaccgc
    16681 gccggtgctg aacgcacagt cttcagaaga gagccagaga atcagcgagg ccgcctcttc
    16741 cggcaacaga aaccggttca ttggaatctt cgagagcatg tagtcgatat gctgctgcga
    16801 catcgaatcg aagatctccg ttttggccgc cgcgggcgtg accgcattga cgaggatgtt
    16861 cttcgtcgca agctctttgc ctagcgattt ggtcaagccg atgagcccgg ctttggacgc
    16921 gctgtagtgc gaggcgttcg ggttgccctc cttaccggcc accgaagcga tattgacgat
    16981 ccgtccgtag ccctgcttga gcatttgcgg tacgaccgcg cggcaggtga gataggggcc
    17041 gatcagattc acgtcgatca cgcggcgcca gacctcgggg tcgagttccc acgtcgtgcc
    17101 gttgccgccg gtgatgccgg cgcagttgat caacacgtcg agcgcgccat gggcggcgac
    17161 cgtttgcgcc accgcctgat ccactgaggc ttcctgggtg agttcgaccg tgacagccgt
    17221 tactttgccc agtccgctga gttcgctcac gctgcgcgcg agccgctcgg cgtcgatgtc
    17281 ccatagggca accgacgcgc ctgaattcag cgcccgttga gccactgcgt agccaatgcc
    17341 gcgcgcgccg ccggtaatgg cgacaactct tccttccaga tcgattcgat tcatcgctgt
    17401 gctcctatgg caggcctcgc cggcttgcct ttcagttgtt gtaattctgc tgtccgtgag
    17461 gcttctgctt tcgttcagca aatatacgag cggtgcgatg cgctaacaat ccgtgtattg
    17521 gagagggtga taacaaaaac ggatcgcggg ggggcttggc ggcgagcgtg cggggttgga
    17581 agtgctctca ctttcgcaag acgcaaaagc cgcttcgatc aaaatccatg caatggcatc
    17641 ccg
//
LOCUS       DATASH010000953         4374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6096243,
            whole genome shotgun sequence.
ACCESSION   DATASH010000953 DATASH010000000
VERSION     DATASH010000953.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6096243"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /locus_tag="VHV99_25450"
     CDS             <1..1098
                     /locus_tag="VHV99_25450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspD"
                     /protein_id="HEX3638767.1"
                     /translation="NLGSGTSSIVDLTAAGATVANNPAALAGQTNILAQGLNIGLLHK
                     FGNFFGLGGLLQALSTSSDANILSTPNLITLDNEEAKIVVGQNVPVVTGSYATPTANT
                     GTSVSAFNTFDRRDVGVTLHVKPQITTGGILKLQLYQEDSSVDNTTINNPGGVTINTR
                     SIQSTILADDGEIVVLGGLMQDQYSTSNSKVPLLGDIPWIGQLFRSEAKTRTKTNLMV
                     FLRPVIVRDQQTSSQISNDRYEYLRQQQYNSTSDNRIEKDKDVPVLPPAPIGPSQGGV
                     PSQNLFDLDNMTRSPSQAGSTTLPQAAPPKVQDQQGQYQGQQQYQTAQPPGQYQGQPQ
                     YQGQPANVNNYAVPNNSAPGVPLNGDAGAKP"
     gene            1095..2582
                     /gene="gspE"
                     /locus_tag="VHV99_25455"
     CDS             1095..2582
                     /gene="gspE"
                     /locus_tag="VHV99_25455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HEX3638768.1"
                     /translation="MNTLNASVANVATSTPSPLAARLIPYAFARTGQILVAHQHADSI
                     EVWISDKTSDAALAEVARNFGALSIVRLPVAELSAAINSAYSRNDGSAAQVVGEVEGE
                     VDLSRLMQDIPEIEDLLESEDDAPIIRMINALLTQAAREQASDIHIEPFETSSVVRFR
                     VDGTLRDVVRPKKALHGALISRIKIMAQLDIAEKRLPQDGRITLRVGGRPVDVRVSTL
                     PTGHGERAVLRLLEKDAQRLNLETLGMARDTLVHFDKLIGRPHGIVLVTGPTGSVKTT
                     TLYAAMSRLETATTNIMTVEDPIEYDLGGIGQTQVNERIGMTFARALRSILRQDPDII
                     MIGEIRDLETAQIAVQASLTGHLVLATLHTNDAASAVTRLTDMGVEPYLLASSLLGVL
                     AQRLVRQLCPVCKEERSEDGHIHWHAVGCDKCGHSGYAGRRGVYELLLLDDPLRALIH
                     RNASDAEILTAGRDQGMRTLREDANRWLATGATSLEEVLRVTGGD"
     gene            2589..3806
                     /gene="gspF"
                     /locus_tag="VHV99_25460"
     CDS             2589..3806
                     /gene="gspF"
                     /locus_tag="VHV99_25460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HEX3638769.1"
                     /translation="MPAFRFEAIDQAGKSQKGVLDADSARAARSQLRTQGLTPLVVEA
                     AATRTRGERKQRLSLGKRLSQREQAILTRQIASLLTAGLPLGETLSVLTDQSERDYVR
                     ELMAAIRGEVLGGHSFANALAEHPRDFPEIYRALVAAGEHTGKLGLVLSRLADYIEQR
                     NALKQKIVLAFTYPAIVTLIAFGIVTFLLSYVVPQVVNVFASTKQALPFLTIAMMALS
                     GFVRNWWWAGLIGVALLSWMIKSLLARPGPRMAFDRWLLTAPLLGKLVRGYNTVRFAS
                     TLAILTAAGVPILRALQAAGETLSNRAMSANVEDAIIRVREGTSLSRALGNTRTFPPV
                     LVHLIRSGEATGDVTTMLDRASEGEARELERRTMFLTSLLEPLLILAMGGVVLVIVLA
                     VMLPIIELNNLVQ"
     gene            3820..4233
                     /locus_tag="VHV99_25465"
     CDS             3820..4233
                     /locus_tag="VHV99_25465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HEX3638770.1"
                     /translation="MNAFQTRLLSLIALAIFCATLTWWVITLTTHQSAPLPAAAAGRA
                     PSVEQAATLFGGQLQHQENQDVHLFGILALQHGAAAIVSYGGEPAKAISLGGPLTPGV
                     TIKEVRSRSIIIDRKGKRSEIFLPPNAPGPTIYVR"
BASE COUNT          863 a         1328 c         1418 g          765 t
ORIGIN      
        1 aacctgggct cgggtacgtc gagcatcgtc gacttgactg cagccggcgc cacggtcgcg
       61 aacaatccgg cggcgcttgc aggccagacg aacatcctcg cgcaggggtt gaacatcggc
      121 ctgctgcaca agttcggcaa cttcttcggc ctgggtggat tgctccaggc gctgtcgaca
      181 tcgtcggatg cgaacatcct gtctacgcct aacctcatca cgctggataa cgaggaagcg
      241 aagatcgtgg tcggccagaa cgtgccggtg gtcaccggtt cgtacgccac gccgaccgcc
      301 aatacgggca cgtcggtatc cgcgttcaat acgttcgacc ggcgcgacgt gggcgtgacg
      361 ctgcacgtga aaccgcagat cacgaccggc ggcattctga agctccagct atatcaggaa
      421 gactccagcg tcgataacac cacgatcaac aatcccggcg gcgtgacgat caacacgcgc
      481 tcgatccagt cgaccatcct ggcggacgat ggtgaaatcg tcgtgctcgg cggcttgatg
      541 caggaccagt attcgacgag caacagcaaa gtgccgctgc tcggcgatat cccctggatc
      601 ggccagttgt tccgcagcga agcgaagacg cggaccaaaa ccaatttgat ggtgttcctg
      661 cgcccggtga tcgtgcgcga tcagcagacc agttcgcaga tatcaaacga tcgctacgaa
      721 tatctgcgcc agcaacagta caactccacc tccgataacc gtatcgaaaa ggataaggac
      781 gtgcctgttc tgccgccggc gccgattggg ccgagccagg gcggtgtgcc gtcgcagaac
      841 ctgttcgacc tggacaacat gacacgctcg ccgtcgcagg ccggcagcac tacgttgccg
      901 caggcggcgc ctccgaaggt gcaggatcaa caaggccagt atcaaggcca gcagcaatac
      961 cagacggcgc agccgccggg ccagtatcaa gggcagccgc agtatcaggg ccagccggcc
     1021 aacgtgaata attacgctgt gccgaataac tcggcgccag gggttccgct caacggcgac
     1081 gcgggagcga agccgtgaac acgctgaacg cgtccgtcgc gaacgttgcc acgagcacgc
     1141 cgtcgccgct cgcggcacgg ctgatcccgt acgcgttcgc gcggaccgga cagatcctgg
     1201 tggcgcacca gcacgcggac agcatcgaag tgtggatcag cgataaaacc agcgacgccg
     1261 cactcgccga agtcgcccgc aacttcggcg cgctttccat cgtgcgtttg ccggtggccg
     1321 aactgtcggc tgcgatcaat tcggcgtact cacgcaatga cggcagcgcg gcgcaggttg
     1381 tgggcgaagt ggaaggcgaa gtcgatctgt cgcggctgat gcaggacatt cccgaaatag
     1441 aggatttgct cgaatcggaa gacgacgcgc cgatcatccg catgatcaac gcgctgctga
     1501 cgcaggcggc gcgcgaacag gcgtcggata ttcatatcga gccgttcgag acgtcttcgg
     1561 tggtgcgctt tcgtgtcgat ggcaccttgc gcgacgtcgt gcgcccgaag aaagcgctgc
     1621 atggtgcgct gatctcgcgt atcaagatca tggcgcaact ggatatcgcg gaaaaacggc
     1681 tgccgcagga tggccgcatt acgctgcgcg taggcggcag gccggtggac gtgcgcgtgt
     1741 ccacgctgcc gaccggtcac ggcgaacgcg cggtgctgcg tctgctggaa aaagatgcgc
     1801 agcggctgaa cctcgaaacg ctgggcatgg cgcgcgacac gctggttcat tttgacaagc
     1861 tgatcggccg tccgcacggc atcgtgctgg taaccggtcc gacgggttcc gttaaaacga
     1921 ccacgcttta tgccgccatg tcacggctcg aaactgccac caccaacatc atgacggtgg
     1981 aagatccgat cgaatacgac ttgggcggga tcggtcagac gcaggtgaac gagcgcattg
     2041 gcatgacctt cgcgcgcgcg ttgcgatcca ttctgcggca ggacccggac atcatcatga
     2101 tcggggaaat ccgcgacctg gaaacggcgc agatcgcggt gcaggcatcg ctgacgggtc
     2161 acctcgtgct ggcaacgctg cacacgaacg acgcggcatc cgcggtcacg cgtctcaccg
     2221 atatgggcgt cgaaccatat ctgctggcgt cgtcattgct cggcgtgctg gcacagcggc
     2281 tggtgcgcca gttgtgtccg gtatgcaagg aagagcgctc ggaagatggc cacattcatt
     2341 ggcacgcggt cggatgcgac aagtgcggcc actcgggtta tgcgggacgt cgcggcgtgt
     2401 acgaactgct gctcctcgac gatccgctgc gcgcgctgat tcatcgaaat gcatcggatg
     2461 ccgaaatttt gacggccggc cgcgatcaag gaatgcgcac actgcgcgag gatgcaaacc
     2521 ggtggctcgc gaccggcgca acgtcgctgg aagaagtgct gcgcgtgacg ggcggagatt
     2581 aaacatccat gccggcgttt cgttttgagg caatcgatca ggcgggcaag tcgcagaaag
     2641 gcgtactcga tgccgacagc gcccgcgcgg cccgcagcca actgaggacg caggggctga
     2701 cgccgctcgt ggtcgaggcg gcggctactc gcacacgggg cgagcgcaaa cagcggttat
     2761 cgctgggaaa acgcttgtcg caacgggagc aagccattct cacgcggcaa atcgccagct
     2821 tgctgacagc gggtttgccg cttggcgaaa cgctgtcggt gctgaccgac caatcggagc
     2881 gcgattacgt tcgcgagctg atggccgcga tccgcggcga agtgctcggc ggccattcgt
     2941 tcgcgaatgc cctggcggaa catccgcgag atttccccga gatttatcgc gcgctggtgg
     3001 cagccgggga acataccggc aagcttggac tggtgctgtc gcggctcgcg gattacatcg
     3061 aacaacgcaa cgcgctgaag cagaagatcg tgctcgcgtt cacctatccg gcgatcgtca
     3121 cgttgatcgc attcggaatt gtcacgttcc tgctgagcta cgtggtgccg caagtggtga
     3181 acgtgttcgc cagtacgaag caggcgctgc cgttcctcac gatcgcgatg atggcgctct
     3241 ccggcttcgt gcggaactgg tggtgggcag ggctgatcgg cgtggcgctg ctgagctgga
     3301 tgatcaagag cctgctcgcg cgtcccgggc cgcgcatggc gttcgaccgg tggctgctca
     3361 ccgcgccgtt gctcggcaaa ctcgtgcgcg gttataacac cgtgcgtttt gccagcacgc
     3421 tcgcgatcct gacagcggcc ggcgtgccaa ttttgcgcgc actgcaagcc gcgggcgaga
     3481 cgctgagcaa ccgcgcgatg agcgcgaatg tggaagacgc gatcatccgt gtgcgcgaag
     3541 gcacgtcgct gtcgcgcgcg ctcggcaata cgcggacatt tccgccggtg ctggtccatc
     3601 tgatccgctc cggcgaagcc accggcgacg tcaccaccat gctcgaccgg gcgtccgaag
     3661 gcgaagcgcg cgaacttgaa cgccgcacga tgtttctcac gagcctgctg gaaccgctgc
     3721 tgatcctggc gatgggcggc gtcgtgctgg tgatcgtgct ggcggtgatg ctgccgatca
     3781 tcgaactgaa taatctggtg cagtgaaaaa gcccatccca tgaatgcatt ccagacccgc
     3841 ctgctatcgc ttatcgcgct cgccattttc tgcgcgacgc tgacgtggtg ggtgatcacg
     3901 ctgacgactc accagagcgc gccattgccc gcggcggcgg cggggcgcgc gccgtcggtg
     3961 gaacaggcgg ctacgttgtt cggcgggcag ttgcagcatc aggagaacca ggacgtgcac
     4021 ttgttcggga tcctcgcgct gcaacacggc gcggcggcga tcgtcagcta cggcggcgaa
     4081 ccggcgaagg cgatctcgct gggcgggccg ctgacgccgg gcgtgacgat caaggaagtg
     4141 cgctcgcgct cgatcatcat cgatagaaag ggcaagcgct ccgaaatatt cctgccgccg
     4201 aacgcgcccg gcccaaccat ttatgtgcgc tgaggcggtc ctggatcaaa cggatcggac
     4261 gaggcgacgc ttacgaagtg aaatccgccc aaccggacct atagaatgct tgcctgcccg
     4321 caacgatcgt tgattgttcc gatgaatggt ccatggtctg aataagggcg gcgg
//
LOCUS       DATASH010000954         7107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_557679,
            whole genome shotgun sequence.
ACCESSION   DATASH010000954 DATASH010000000
VERSION     DATASH010000954.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7107
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557679"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="VHV99_25470"
     CDS             complement(<1..696)
                     /locus_tag="VHV99_25470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX3638771.1"
                     /translation="MTSRNFSIETIGIVGTGAMGRGIAQIAALAGLTVRLYDTNPAAI
                     GAARDYLAETFAKLTAKGKLDQARSLAALANVSGAQVIGDLADCDLVVEAIVEKLEVK
                     QALFRELEAVVSGRCLLASNTSSLSITAIAAGCTNPSRVAGYHFFNPVPLMKVVEVIH
                     GLRTDPAAGDALMNLARRMGHTPVRAKDMPGFIVNHAGRGMNTEGLRVASEGVASFAD
                     IDRIMREQAGFRLG"
     gene            824..1471
                     /locus_tag="VHV99_25475"
     CDS             824..1471
                     /locus_tag="VHV99_25475"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006052875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEX3638772.1"
                     /translation="MIKLCGFALSNYYNKVKFVLLEHGIPFEEVFVLPSQEATMLEHS
                     PLGKVPYIQTEHGDLCESQSIVEYLAARFPDKAIFSPDPWEAAKERELIMFVDVHLEL
                     TARNLYKQAFFGGTVTDATKGRVEKLLAHHIAGFRRIAKFGPYLRGEHFSVADAAGYV
                     SLPLVGMATQTVYGRDFLLDAGVDWKSYVKVINARPAAQRVTEDRKAYIAASRPA"
     gene            complement(1581..2753)
                     /locus_tag="VHV99_25480"
     CDS             complement(1581..2753)
                     /locus_tag="VHV99_25480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006025129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX3638773.1"
                     /translation="MNAPHDTPMSPSFPSRLPDVGTTIFTVMSALAAEKGAVNLGQGF
                     PDFGCDPRIVDAVANAMRDGHNQYPPMAGAAPLRQAISDKIANLYGRRYDATSEITVT
                     AGATQALLTAILCTVHPGDEVIVVEPTYDSYLPSIELAGGKPVFVTLEAPDYKIPFDK
                     LAAAITPKTRLLLINTPHNPTGTVWRSEDMRKLEEIVRGTNVLILSDEVYEHMVYDGA
                     PHESVARYPELAQRSFVVSSFGKTYHVTGWKVGYVAAPAALTAEFRKVHQFNVFTVNT
                     PMQVGLAEYMKDPAPYLDLPAFYQKKRDFFRAGLANSRFKLLPCTGTYFQCVDYSAIS
                     DLPEADFAQWLTGEVGVAAIPVSAFYHEAHESGVVRFCFAKQEATLATALERLARL"
     gene            complement(2890..3381)
                     /locus_tag="VHV99_25485"
     CDS             complement(2890..3381)
                     /locus_tag="VHV99_25485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HEX3638774.1"
                     /translation="MPGPHASHGALRVVLDSNVWIDILVFDDPHTRPIRAALESGALA
                     ALIDARCLAELAYVLDYPQFVHRNVDKAAALAAVARLAQLVEPAAPAEDARPLPKCKD
                     RDDQKFLELAHAAQAHWLVSKDRAVLKLARRIARDFGFQIAQPAPFVSACMSAADEPA
                     PAV"
     gene            3439..4221
                     /gene="yaaA"
                     /locus_tag="VHV99_25490"
     CDS             3439..4221
                     /gene="yaaA"
                     /locus_tag="VHV99_25490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEX3638775.1"
                     /translation="MIIVLSPAKSLDYETPPHVKKHTIPDFVDDAAELIGGLRRLSPQ
                     QIGSLMSISDQLAHLNFQRYAEWSPSFGTHNAKQAVLAFNGDVYEGFNARTLSSADLD
                     YAQSHVRVLSGLYGLLRPLDLLQPYRLEMGTRFVNARGKDLYAFWGERITQALNAQLK
                     KNTVASRVLVNCASGEYFKSVKPKLLDAPIVTPVFEDWKGGRYKIISFHAKRARGLMA
                     RYAVENRLDKPEQLKDFDSEGYAFDAEASNDSTYVFRRRIAD"
     gene            4256..5410
                     /locus_tag="VHV99_25495"
     CDS             4256..5410
                     /locus_tag="VHV99_25495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14-type cytosolic carboxypeptidase"
                     /protein_id="HEX3638776.1"
                     /translation="MTLSITSNFDAGAIEVLACEDAGNIRLRVRPDSHAEFAQWFYFR
                     LSGAAGERCVMTFENAAACAFAEGWRDYQAVASYDRVNWFRVPTSYDGRVLTIDHTPD
                     FDRIYYAYFEPYGEERHSEFLGAVQQLPQATLTELGKTVEGRPMSLLTLGTPQTGDTP
                     RKKIWLIARQHPGETMAEWFIEGLVKRLVGWGDWVGDPVARKLYDHAVFYIVPNMNPD
                     GSVHGNLRTNATGANLNREWMEPDAARSPEVLVVRDAIHATGCDLFFDIHGDEALPYV
                     FVAGSEMLPGFTERQGEEQKAFIEAFKHASPDFQDKYGYAASKYRQDALKLASKYIGN
                     EFGCLSLTLEMPFKDNANLPDERVGWNGERSASLGAAMLQAILRHVETFA"
     gene            complement(5528..6196)
                     /locus_tag="VHV99_25500"
     CDS             complement(5528..6196)
                     /locus_tag="VHV99_25500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638777.1"
                     /translation="MDRTNVPRRLLRLVGRLATFVASLSLICTAPAYADQLEQHNDLV
                     NKFVNDMHADPLVADCAAHGDFVASTSTVFDHVEFPPSSFDSAHASVTPWNDSFDEGK
                     QRVKVDNIVTVEGLGVRQSGDGDPADLKFRCGYVGTQMLAFSWNDPVPPARAHSSGSA
                     GSQASSGKHKSVKGKHAAGGKTATKKATGKSSKASSGKSSRATSSKSSSKSAAKKSAA
                     KKTQ"
     gene            complement(6363..7037)
                     /locus_tag="VHV99_25505"
     CDS             complement(6363..7037)
                     /locus_tag="VHV99_25505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HEX3638778.1"
                     /translation="MGFLYTPLPFWVAVGGWIATAIVVALALWKNPFKRLQDGTLQHV
                     WLAIIVAVSVLWASNAWLDDGTVMHLLGATLVVTLFDWALALIAMAIVTGLAAVIFDA
                     PWQGIALTFLVFGALPVGISTLIQRASIAWLPRNLFMFIFGQGFVSPAIAVSLTAAAA
                     LEIHIALADGSMTVVPAGYAFSVLLLATGEAWFTGMSTALIAVYRPAWVTTYDVRRYR
                     LGGPRT"
BASE COUNT         1372 a         2091 c         2277 g         1367 t
ORIGIN      
        1 cccgagacga aagcctgcct gctcgcgcat gatacggtcg atgtcggcga agctcgccac
       61 gccttcgctc gccacgcgca ggccctctgt attcatgccg cggccggcat ggttgacgat
      121 gaagccgggc atgtctttcg cgcgcaccgg cgtgtggccc atgcgacgcg cgagattcat
      181 cagcgcgtcg ccggcggcgg gatcggtgcg cagtccgtgg atcacttcga cgaccttcat
      241 caacggaacc ggattgaaga aatggtaacc ggctacgcgt gacggattcg tgcagcccgc
      301 cgcaatcgcg gtaatcgaca acgacgacgt gttcgacgcc agcaggcagc gcccgcttac
      361 cacggcttcg agttcgcgaa agagggcttg cttcacttcg agcttctcga cgatcgcctc
      421 gacgaccaga tcgcaatcgg ccagatcgcc gatgacctgc gcgccgctca cgttcgccag
      481 cgcggcgagc gagcgggcct gatcgagctt gcctttggcg gtcaatttgg cgaaagtctc
      541 ggcaaggtag tcgcgcgcgg cgccgatcgc ggcgggattc gtgtcgtaca gacgcacggt
      601 aaggcccgcc agtgcggcaa tctgggcgat gccacgaccc attgcaccgg ttccgacaat
      661 gccgatagtc tcgatactga aattgcgaga ggtcattgcg atgctcgact ccatggttat
      721 tttagaatag cacgatcgtg caatttaccg gatgattgat cgaaggccgc cgcttttgat
      781 gtcgacgagc ggtctcatac aacccgaagg aaggagacac ccgatgatca aactgtgcgg
      841 tttcgcgctc tcgaactatt acaacaaggt gaagttcgtg cttctcgaac acggcattcc
      901 gttcgaggaa gtgttcgtgc tgccgagcca ggaagcgacg atgctcgagc attctccgct
      961 cggcaaggtg ccctatatcc agaccgagca cggcgatctg tgcgaatcgc aaagcatcgt
     1021 cgagtatctg gccgcgcgtt ttcccgacaa ggcgattttc tcccctgacc catgggaggc
     1081 ggccaaggag cgcgagttga tcatgttcgt ggacgtgcat ctcgaactga ccgcacgcaa
     1141 tctctacaag caggcgttct tcggcggtac cgtgacggac gcgaccaagg ggcgcgtcga
     1201 gaagctgctc gcgcatcaca ttgccggctt caggcggatc gcgaagttcg ggccgtattt
     1261 gcgcggcgag catttcagtg tcgccgacgc ggcgggttat gtgagcttgc cgctggtcgg
     1321 catggcgacg cagactgtct acgggcgcga cttcctcctc gacgccggtg tggactggaa
     1381 aagctatgtg aaggtgatca acgcccggcc ggccgcgcag cgcgtgaccg aagatcgcaa
     1441 ggcgtatatc gcggcctcgc gtccggcctg acgtacgacg gtgtggcggc tgaaatgaat
     1501 gcgcgcgcgc gacgcacgca taggccgttg cgcgcgatgg cgaggaacgt gggttcgttc
     1561 gcagcatcgc gcgggacatc ctagagccgc gccagccgct cgagcgcggt ggcgagtgtg
     1621 gcttcctgct tggcaaagca gaaacgcacc acgcccgatt cgtgggcttc gtgatagaac
     1681 gccgagaccg gaattgccgc gacaccgacc tcgccagtca gccattgcgc gaaatcggct
     1741 tcgggcagat cgctgatcgc cgaatagtcg acgcactgga agtacgtgcc cgtgcaaggc
     1801 agcagcttga agcgggaatt cgcgaggccg gcgcggaaga agtcgcgttt cttctgataa
     1861 aacgcgggca gatccagata cggtgccgga tccttcatgt attcggcgag gccgacctgc
     1921 atcggcgtgt tgaccgtgaa cacgttgaac tggtgcacct tgcggaattc agcagtgagc
     1981 gcggcaggcg cggccacgta gccgaccttc cagccggtga cgtgataagt cttgccgaag
     2041 ctcgacacca cgaaactgcg ctgtgccagt tccggatagc gcgccacgct ttcgtgcggc
     2101 gcgccgtcgt acaccatgtg ttcatagact tcgtcggaca gaatcagcac gttggtgccg
     2161 cgcacgattt cctccagctt gcgcatgtct tcgctgcgcc agactgtgcc ggtcggattg
     2221 tgcggcgtgt tgatcagcag gagccgcgtt ttcggcgtga tcgcggccgc gagcttgtcg
     2281 aacggaatct tgtagtcggg cgcttcgagc gtgacgaata ccggcttgcc gccggccagc
     2341 tcgatcgacg gcaggtagct gtcgtaggtc ggctcgacca cgatcacttc gtcgcccgga
     2401 tgcacagtgc agagaatcgc cgtcagcaac gcctgggtgg cgcccgccgt cacggtgatc
     2461 tcgctggtgg cgtcgtagcg gcggccgtag aggttcgcaa tcttgtccga aatcgcctga
     2521 cgcagcggcg cggcgccggc catcggcggg tattgattgt ggccgtcgcg catggcgttc
     2581 gcgaccgcgt cgacaatgcg cggatcgcaa ccgaagtcgg gaaagccctg gccgaggttc
     2641 accgcgcctt tttcggcggc gagcgcactc atcacggtga agatcgtcgt gccgacgtcc
     2701 ggcaggcgag acggaaacga gggactcatg ggcgtgtcgt gcggtgcatt catggcgctg
     2761 tccggagcgt ggtcgagtga attggcgaaa ggcgtcgttt gaaaaggacg aagcctgatt
     2821 gtagggaaaa cgcggcgctg cgtgcgcgcc gtcggcgcaa cgttggccat ccggccaggt
     2881 gtcatggcgt catacggcgg gcgcgggttc gtctgccgcg ctcatgcagg cgctgacgaa
     2941 cggcgccggt tgtgcaatct gaaaaccgaa gtcgcgcgca atccgcctgg caagtttcaa
     3001 caccgcgcgg tctttcgaca ccagccagtg ggcctgcgcg gcgtgcgcga gttcgaggaa
     3061 tttctggtcg tcgcggtctt tgcatttggg cagcggcctc gcatcttccg cgggtgccgc
     3121 gggctcgacc agctgcgcga gccgtgcgac agcggcaagt gcggccgcct tgtcgacatt
     3181 ccgatgcacg aattgcggat agtcgagcac gtaggcgagt tcggcgaggc agcgcgcgtc
     3241 gatcagcgcg gccagcgcgc cactttcgag cgcggcgcgg atcggccgcg tgtgcggatc
     3301 gtcgaacacg aggatatcga tccagacatt ggagtcgagg acgacgcgca acgcgccgtg
     3361 tgaggcatgg ggaccgggca ttcgttacaa tctggctttc gtctttgacc gccaggcacg
     3421 gcgtgcctgc aagcctctat gataatcgtt ctgtcgccgg cgaaatcgct cgactacgaa
     3481 acccctccgc acgtcaaaaa gcacacgatc cccgatttcg tcgacgacgc ggccgaattg
     3541 attggcgggt tgcgccgttt gtcgccgcag cagatcggct cgctgatgag catttccgat
     3601 cagcttgcgc acctcaattt ccagcgttac gcggagtggt cgccgagctt cggcacgcac
     3661 aatgccaagc aggctgtact cgcgttcaat ggcgacgtgt acgaaggctt caacgccagg
     3721 acgttgtcgt cggccgatct ggattacgcg caaagccacg tgcgcgtgct gtccggcctg
     3781 tacggactgc tgcgcccgct cgacctgctg cagccgtacc ggctcgaaat gggcacgcgc
     3841 tttgtcaacg cgcgcggcaa ggatctgtac gcgttctggg gcgagcggat cacgcaggct
     3901 ttgaacgcgc aactgaagaa gaacaccgtt gcctcgcgcg tgctggtcaa ctgcgcttcg
     3961 ggtgagtact tcaagtcggt caagcccaag ctcctcgacg cgccgatcgt cacgccggtg
     4021 tttgaagact ggaagggcgg ccgctacaag atcatcagct tccacgcaaa gcgcgcgcgc
     4081 ggtctgatgg cgcgctacgc ggtcgagaac cgcctcgaca agcccgaaca gttgaaggac
     4141 ttcgactccg aaggctatgc gttcgacgcc gaagcctcga acgattccac ttacgtattc
     4201 cgccgccgca tcgctgacta ggcgattgcc gcgcacacaa gcagggaagc tcatcatgac
     4261 gttatcgatt acgagcaact tcgacgccgg tgcgatcgaa gtgctggctt gtgaagacgc
     4321 tggcaacatc cgtctgcgcg tacggcctga tagtcacgcc gagtttgcgc agtggtttta
     4381 tttccgtttg tccggcgcgg ccggcgagcg ctgcgtgatg accttcgaaa atgcagcggc
     4441 gtgtgccttc gccgaaggct ggcgcgacta tcaggcggtg gcaagctacg accgggtgaa
     4501 ctggttccgc gtgccgactt cctacgacgg ccgcgtgctg acgatcgacc acacgcctga
     4561 tttcgaccgc atctattacg cctatttcga gccgtatggc gaggaacgtc actctgagtt
     4621 tctcggcgcg gtccagcagt tgccgcaggc cacgctcacg gaattgggca agactgtcga
     4681 aggccggccg atgtcgctgc taacgctggg cacgccgcaa accggcgaca cgcccaggaa
     4741 gaagatctgg ctgatcgcgc gtcagcatcc tggtgagacg atggccgaat ggtttatcga
     4801 aggtctcgtc aagcgtctgg tgggctgggg cgattgggtg ggcgacccgg tggcgcgcaa
     4861 gctctacgat cacgcggtgt tctacatcgt gccgaacatg aatccggacg gcagcgtaca
     4921 cggcaatctg cgcaccaacg cgaccggtgc gaacctgaac cgcgaatgga tggagccgga
     4981 cgccgcgcgc agtccagagg tgctggttgt gcgtgacgca attcacgcca ccggttgtga
     5041 tctctttttc gatatccacg gcgacgaagc gctgccgtac gtgttcgtgg ccggctctga
     5101 aatgttgccg ggtttcaccg agcggcaggg cgaggagcag aaggcgttta ttgaggcgtt
     5161 caagcatgcg agtcccgact tccaggacaa gtatggctac gccgcgagca aatatcgcca
     5221 agatgcgctc aagctggctt caaagtacat cggcaatgaa ttcggctgtc tgtcgttgac
     5281 gctggagatg ccgttcaagg acaacgccaa cctgcccgac gagcgggtgg gttggaacgg
     5341 cgaacgtagt gcgtcgctgg gcgccgcgat gctgcaggcg atcttgcggc acgtggagac
     5401 gtttgcctga cctgacgtta cctcatagac aaacggggca cccactttgg aagtgggtgc
     5461 cccgtttctt ttgatgtttg catggcccgc ggcccgcgtc agttgaatcc gctacggggc
     5521 tggccagtta ttgagtcttt ttcgcagcag atttcttcgc ggccgatttg ctcgacgact
     5581 tgctggaagt cgctcgcgaa gattttcccg agcttgcttt cgaggacttg ccggtcgctt
     5641 tcttcgtcgc cgttttgccg ccagcggcgt gtttgccctt cacgctctta tgcttgcccg
     5701 aggacgcttg cgatcccgcc gatccggaac tatgcgcacg ggccggtggc accggatcgt
     5761 tccagctgaa cgcgagcatc tgggtgccta catagccgca acggaacttg agatcggctg
     5821 gatcgccgtc gccgctctgg cggacaccga gaccttcgac ggtcacgatg ttgtccacct
     5881 tgacacgctg cttgccctcg tcgaacgaat cgttccaagg cgtgaccgac gcatgcgcgc
     5941 tgtcgaagga gctgggtgga aattcgacat ggtcgaagac ggttgatgtg cttgcaacaa
     6001 aatcgccatg cgcagcgcag tccgcgacta acgggtctgc atgcatgtca ttgacaaatt
     6061 tgttgacgag atcgttgtgc tgttcgagtt gatcggcgta tgcgggagca gtgcaaatga
     6121 gcgagagact cgctacaaat gttgctaacc ggccgaccag ccgcagcaat cggcgcggta
     6181 cgttagtgcg atccatctag ttcgttagac gcttaggaga cgtgaggcac gtaagatgca
     6241 cagcgagagg gatgagttca atcctgacac aaatattttg tcctgtcgtg gtgcgcagac
     6301 tcaagcgctt acgtgccggg atggctgatt gccgcagata ggtgctcgat gccgcgtctc
     6361 tctcaggtgc gtggtccgcc caatcgataa cgacgtacat cataagtggt gacccacgcc
     6421 ggacgataga ccgcgatcaa tgccgtcgac atgcctgtga accaggcctc gccggtggct
     6481 agcagaagca cgctgaacgc gtagccggcc ggcaccacgg tcatcgaacc gtcggccagc
     6541 gcgatgtgaa tttcaagtgc cgcggccgcc gtgagggata cggcaatggc gggtgacaca
     6601 aaaccttggc cgaagatgaa catgaataga ttgcgaggca gccaggcaat gctggcgcgc
     6661 tggatcaagg tcgagatgcc aaccggcagc gcgccgaaca cgaggaaagt cagcgcaatg
     6721 ccctgccagg gggcgtcgaa gataacagcg gcgagaccgg tgacgatcgc catggcgatc
     6781 agcgccaacg cccaatcgaa tagcgtgacg accaacgtgg cgccgagcag atgcatgacc
     6841 gtgccgtcgt cgagccacgc gttgctcgcc cacagcaccg acaccgcgac gatgatcgcg
     6901 agccagacat gttggagcgt gccgtcttga agtcgtttga aaggattttt ccacagcgcg
     6961 agcgcaacca ctatcgcagt ggcgatccag ccaccgacag cgacccagaa cggaagcggt
     7021 gtgtagagga aacccatgta tctcatatta ctcgttggac gagaaaaggt gtgagcggat
     7081 tccatgccag ccggcgcaaa cgctgcc
//
LOCUS       DATASH010000955         2241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2141003,
            whole genome shotgun sequence.
ACCESSION   DATASH010000955 DATASH010000000
VERSION     DATASH010000955.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2141003"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VHV99_25510"
     CDS             <1..220
                     /locus_tag="VHV99_25510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3638779.1"
                     /translation="ADGQKEALNLIRQGKYGATALNDPALVARTAVDIGMKAAKGEST
                     NVPKISYTPSAVINQSNVDKFYNPKAIF"
     gene            286..1794
                     /locus_tag="VHV99_25515"
     CDS             286..1794
                     /locus_tag="VHV99_25515"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEX3638780.1"
                     /translation="MIPFIALTGVDKRFGGIHAIEDVDFDVLGGEVHALVGENGAGKS
                     TLMRIIGGGHQPDAGTIKVEGREMSLRNPHAAMEEGIAVIHQETALAPDLSVAENVFL
                     GALPAVIDWRSLRKRAHVLIASLGFDIDPSALVGSLSTAQCQVIEIAKALSRQVKLLV
                     LDEPTAALAPSDANKLLEIVRDLRSRGVGIVYISHRLEEVFAIADRITVLKDGKRVDT
                     VKPADLNMDELIRKMVGRPLSVLFPQRHATLGDEVLKVTGLVRGHAVRNVSFTLRAGE
                     VVGLGGLIGSGRTEVARLIFGADTRDSGTIELKQKLLAIRSPMAAVKAGIGFVPEDRK
                     GQGAVLSMPIRVNATLAALKSVSRPGGFLAFGRERAFVQKLMDQLRIKARSMDADVST
                     LSGGNQQKVVLAKWFHADGDLIILDEPTRGVDVGAKVEIYTLINQLAERGKAVLVISS
                     EHQELMGLCDRILVMGEGEIRGELQPADFSEEQILSLSLRRAPDEAPATLTH"
     gene            1817..>2241
                     /locus_tag="VHV99_25520"
     CDS             1817..>2241
                     /locus_tag="VHV99_25520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3638781.1"
                     /translation="MNPSAAAQKNQKTQKTHQAQTGQARHWWSVARRFNTVAILIVLV
                     IAASFVSGDFFSVANLSNISRQVAGVGIMSVGMLLVVLTGGIDLSVGSIAALGSVVSA
                     MLIPQYGLSVALLVTLLVGGVCGFVSGALIAWFRLTPFVV"
BASE COUNT          415 a          672 c          699 g          455 t
ORIGIN      
        1 cgccgacggt cagaaggaag cgctcaacct gatccgccaa ggcaagtacg gcgcgaccgc
       61 gttgaacgat ccggcacttg ttgcacgcac ggctgtggac attggcatga aggcggcgaa
      121 gggcgaatcc accaacgtgc cgaagatttc gtacacgcct tccgccgtga tcaaccagag
      181 caacgtcgac aagttctaca acccgaaagc gatcttctaa agactcgcgg gttcatgcaa
      241 cgcgcgccgg ccccgccggt gcgcgtgcat tctggagaca ccttcatgat tccgtttatc
      301 gccctgaccg gcgttgacaa gcgcttcggc ggcattcacg ccattgaaga cgtcgacttc
      361 gatgtccttg gcggcgaagt ccatgcgctc gttggcgaga acggcgcggg taagtcgacg
      421 ctgatgcgca ttatcggcgg cggccatcaa cccgatgccg gaacgatcaa ggtcgaagga
      481 cgtgagatgt ccttgcgcaa tccgcatgcg gccatggaag aaggcattgc ggtgattcat
      541 caggaaacgg cgttggcgcc ggatttgtcc gtggctgaaa acgtgttcct cggcgcgttg
      601 ccggccgtga tcgactggcg ttcattgcgc aagcgcgcgc atgtattgat cgcgagtctc
      661 ggctttgaca tcgacccttc agcgctggtc ggcagcctgt ccacagcgca atgccaggtg
      721 attgaaattg ccaaggcgct gagccgccag gtgaagctgc tggtgctgga cgaacccact
      781 gccgcgctcg cgccgtccga tgccaacaag ttgctcgaga tcgtgcgcga cctgcgttcg
      841 cgcggcgtgg gcattgtgta tatctcgcat cggctggagg aggtatttgc gattgcggac
      901 cgcatcaccg tgctcaagga tggcaagcgc gtggacaccg tcaagcctgc tgatctcaac
      961 atggacgaac tcattcgcaa gatggtcggc cgcccgcttt cagtgctgtt tccgcagcgt
     1021 cacgcgacgc tcggcgacga agtgctgaag gtcacgggcc tcgtacgcgg tcatgccgtg
     1081 cgcaacgtgt cgttcacgtt gcgcgccggc gaagtggtcg gtctgggtgg cctgatcggc
     1141 tcggggcgca cggaagtcgc ccggctgatc ttcggcgcgg acacccgtga ttccggcacc
     1201 atcgaattga aacaaaagct gctggcgatc cgctcgccaa tggcagcggt caaggccggc
     1261 atcggctttg ttcctgaaga ccgtaaaggc caaggcgcgg ttttatcgat gccgatccgc
     1321 gtcaacgcca cactcgccgc gctcaaatcc gtcagccgcc cgggtggctt tctcgccttc
     1381 ggccgcgagc gtgcgttcgt tcagaagttg atggaccaac tgcgcatcaa ggcccgttca
     1441 atggacgccg atgtctccac gctatcaggc ggcaaccagc aaaaagtcgt gctcgccaaa
     1501 tggtttcatg cggacggcga cctgatcatc ctcgacgaac ctacgcgcgg tgtcgacgtg
     1561 ggcgccaagg tggaaatcta tacgctgatc aatcaacttg ccgaacgggg caaggcggtg
     1621 ctcgtgatct cgtcggagca ccaggagttg atgggcctct gcgaccgcat cctcgtgatg
     1681 ggcgaaggcg agattcgtgg cgaactccag cccgccgatt tcagcgaaga gcagatcctc
     1741 tcgctctcct tgcgtcgcgc gccggacgaa gcgccggcaa cgcttactca ctaaaccaga
     1801 cacggagact cgcatcatga atcccagcgc tgcggctcag aagaatcaga agactcagaa
     1861 gacgcatcag gcgcagaccg gccaggcgag acattggtgg tcggttgcac gccgcttcaa
     1921 cacggttgcg atcctgattg tgctcgtgat cgccgcgtct ttcgtatcgg gcgatttctt
     1981 ctcggtggct aatctgtcca acatctcgcg tcaggtcgcg ggcgtcggca tcatgtcggt
     2041 gggcatgctg ctggtcgtgc tgaccggtgg tatcgacttg tcggtcggct ccattgcagc
     2101 gctgggcagc gtggtgtcgg ccatgcttat tccccaatac ggcctgagcg tcgccctgct
     2161 cgtcaccctg ctggtcggtg gtgtatgcgg ctttgtatcg ggcgcgttga tcgcgtggtt
     2221 ccgtctcacg ccgtttgtcg t
//
LOCUS       DATASH010000956         2099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3524291,
            whole genome shotgun sequence.
ACCESSION   DATASH010000956 DATASH010000000
VERSION     DATASH010000956.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3524291"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..913)
                     /locus_tag="VHV99_25525"
                     /pseudo
     CDS             complement(1..913)
                     /locus_tag="VHV99_25525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612082.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1002..2006)
                     /locus_tag="VHV99_25530"
                     /pseudo
     CDS             complement(1002..2006)
                     /locus_tag="VHV99_25530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT          374 a          704 c          562 g          459 t
ORIGIN      
        1 ccgtccatgg acaagcggat gccccggctg agcacggcct cggtgaacac tgtcgcggtg
       61 aactggctgc cctggtcggt attgacgatc tcaggttgcc cgtacttcgc gaacgcttct
      121 tccaacgcct cgacagcgtg catcgcctcc atcgtgatcg ctacgcggtg ggccagtacc
      181 ttccggctgg cccagtccac cacggcgctc aggtacacga agccacgcgc catcggtata
      241 taggtcgtat ccagtgccca gacctggttt gcccgattga tcgtcatgcc ccgcagcaga
      301 tacggccaga tcttgtgctg cgcattgcgt cggctcgtgt tcggcttgca gtacagcgcc
      361 tcgataccca tgcgcttcat cagcgtgcgc acgcgcctgc ggccaatctc gggtccctcg
      421 cgacgcagca gtcgcgccag catccgcgcc ccggcaaacg gaaactccat gtgcagttca
      481 tcgatccggc gcatcagcaa ctgatctgcc tcgctcaccg gttgcgcccg gtaatacacg
      541 ctcgatcttg cgataccaac gagtcgcgcc tgctgccaga ccggcaatgc gtgcgtacga
      601 tcgatcatcg ctttgcgctc agcaatcccg ctttgttgag cgcgccgctt aaaaaatcgt
      661 tctccagcgt gagctggccg atcttcgcat gtagctcctt caaatcgact tgcggctcgc
      721 tcgacgccgt gccccctgcg ccaaacacgt cggcggcacg ttcctgcaac tgccgcttcc
      781 attcggtgat ctggttcggg tgcacatcga actgctgcgc tagttcggct agcgtccgct
      841 cacccttgac cgccgccatc gccactttcg ctttgaacac ggctgagtgc gtccgtcggg
      901 ttctcttcgt catcttcaag gttcctttgt cggcattatc gccggctcag gccccgagcg
      961 ttccacttat ccgactgtcc gaatttgcgc ggccacctct caggcccgtt tttcgtgcgc
     1021 ggaattagcc catagccttt ttcgcgaggt aagtgcccaa gcatcgtaaa gcggcttgat
     1081 ctttccacca acgtcccgat ggctgatcgg tttaagccga taatcaaatc accctcccaa
     1141 tggcctggca cagcacgatc ttgcacctct gcaggccggc tggaaatcat cacctcctcg
     1201 ctgacgtgcg cccacgccct ggcgcgcgct ctcgcccggg gaacgcgcaa cgcgcgcccg
     1261 gtgcgaaggc aacccaccag ctcgcgcttg agcgcgccgc gcccttgaat atagagcgct
     1321 tgataaatgg cttcgtgaga gatgcgcatg gactcatcat ccgggaaatc agcctgcaac
     1381 cgattagcaa tttgttcagg tgaccagccg ttgacccact ttcggtcacc gcgatgtggc
     1441 ttgtttcgcc ccttgaacgg tgcctgtctg ggcccggcaa tttcacgacc attggcgtca
     1501 cgtactttgc cctccaggtg gtcctgaacg tattggcgca gacgtgcatt agtcgccagc
     1561 ttcgctggtt ttggtcgttt ggcgaaccgt tccgccttcc actgcgcgac cgaagcacga
     1621 tactcgagac acccattacg tgtcgccgcg ttgcgcgtga gctctcgcga aacggttgat
     1681 ggactgcgtc caatatgtcg ggcgatctcg cgcaccccga ggccttggac cgaaagcaat
     1741 gctatctctt ctcgctcagc gaaccacaga tatcgaccag cgacaggctt cgacatgaat
     1801 agtggcatgc cgccacgata gcggaaccag cgcgagccta ccgcttgaga tacgccggcc
     1861 gcctccgctg ctttctcgct tgtaatgccg gttgcgatct gttgccaaaa gcgacgctcg
     1921 acctcatttc taagcgacgg cgcacccggt gagtacatcg cctctcgccc tgtcagccga
     1981 tacacctgct ttgcacttcg tgccatgcaa cacctcctcg atcaaaggtg ttgcgacgac
     2041 cggttgaatc cgccaacacg ggactccgtc gagttggcaa cactggaatg ggtggcctg
//
LOCUS       DATASH010000957         1862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1944814,
            whole genome shotgun sequence.
ACCESSION   DATASH010000957 DATASH010000000
VERSION     DATASH010000957.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1944814"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VHV99_25535"
     CDS             complement(<1..347)
                     /locus_tag="VHV99_25535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX3638782.1"
                     /translation="MRFEGTSGYVATEDLKVAVNAAITLERPLLVKGEPGTGKTVLAL
                     EVAKALNAPLIEWHVKSTTKALQGLYEYDAVSRLRDSQLGDTRVHDIRNYIKRGKLWD
                     AFVSDERPVLLID"
     gene            595..>1862
                     /locus_tag="VHV99_25540"
     CDS             595..>1862
                     /locus_tag="VHV99_25540"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HEX3638783.1"
                     /translation="MGIFDALTTAVTGLQAQSFALQNISGNIANSQTTGFKETNTSFQ
                     DLVTSASPNAQTSGSVLASSVATNSVQGAIQSEQVSTYMAINGDGYFTVAQPTSVTGN
                     APVFSGVNLYTRRGDFQENSQGFLVNGAGYYLMGIPIDRTTGNQVGSVPQVLQFGNNE
                     IPAQVTTQIQYQANLPTVPHTTNANASIPGSELINPVDFTSNPLLLPATIRGSGATLQ
                     PDAPAVDTGTTDISGLPTGGNAGTLQINGTNITINATDSATTIVQKINAVTGTTGVTA
                     TLNPSNNLVLTSKDALTNISTAGTVPPALVTELGLATTANATNLLNQGAVTSGQTLTI
                     TVGTTNPTLTVTFGTNAGDVQTLAQLNAKLAALVGGKASVKAANGDLTIAADNLTDQV
                     TVGGTANTANFGVNTLTASPPTVGPVIGNDV"
BASE COUNT          372 a          633 c          557 g          300 t
ORIGIN      
        1 tcgtcgatca gcagcaccgg ccgctcgtcg gagacgaacg cgtcccacag cttgccgcgc
       61 ttgatataat tgcggatgtc gtgcacgcgc gtgtcgccca gctggctgtc gcgcagcctt
      121 gagaccgcgt cgtattcgta gagcccctgc agcgccttgg tggtcgactt gacgtgccat
      181 tcgatcagcg gcgcgttgag cgccttggcg acttccaagg cgagcacggt cttgccggtg
      241 cccggctcac ccttgaccag cagcggccgc tcgagggtga tcgcggcgtt caccgccact
      301 ttcaaatctt cggtggcgac gtagccgctg gtgccttcga aacgcataag agcctctggc
      361 aagagattgc ttggcgcggc gaccataagg agcacgcgcc gtcacggcaa ggatcggcag
      421 aagttgccgg ccccggcgca tcctgcccag cgggcggccg ggcgctaatg accaagcttc
      481 tgtttttacg cgcctttcga catggcacga ggtttgcgac ggctgcgcca gccatgcggc
      541 cgccgcgtcg agccccgacg cgacggcgct gctgcgatgg caatggaggc ctcgatgggc
      601 attttcgacg ctctcaccac cgcggtgacg ggtttgcagg cgcagtcgtt cgcgctgcag
      661 aacatttcgg gtaacatcgc gaattcgcag acgaccgggt tcaaggaaac caacacgagc
      721 tttcaggacc tcgtcacgtc tgcctcgccc aacgcgcaga cctcaggcag cgtgctcgcc
      781 agctccgtgg caacaaacag cgtgcagggt gcgatccaga gcgagcaggt gagcacctac
      841 atggcgatca acggcgacgg ttatttcacc gtcgctcagc ccacctcggt cacgggcaat
      901 gcaccggtgt tcagcggcgt gaacctctac acccggcgcg gcgatttcca ggaaaacagc
      961 cagggcttcc tggtcaacgg cgccggctat tatctgatgg gcatcccgat cgaccggacc
     1021 accggcaatc aggtgggcag cgttccgcag gtgcttcagt tcggcaacaa cgagattccc
     1081 gcgcaggtga cgacacagat ccagtatcag gcgaacctgc ccacggtccc gcacacgacg
     1141 aacgcgaacg caagtatccc gggctcggag ctgatcaatc cggtcgactt cacgtcgaat
     1201 ccactgctgt tgcccgcgac catccgcggc agcggcgcga cgctgcagcc ggacgctcct
     1261 gccgtcgaca ccggcacgac cgacatttcg ggccttccca cgggcggcaa tgcaggaacg
     1321 ctgcaaatca acgggacgaa catcacgatc aacgccactg actcggcgac caccatcgtg
     1381 caaaaaatca atgccgtgac cggcacgacc ggcgtcacgg cgacgctcaa tccctcgaat
     1441 aatctcgttc tcaccagcaa agacgccctg acgaacatca gcaccgccgg taccgttccg
     1501 ccggcgctag tgacggaact cggtctcgcc acgaccgcca atgccacgaa tctccttaac
     1561 cagggggcgg tgaccagcgg acaaaccctg acgattacgg ttggcacgac caatccgacg
     1621 ttgaccgtca ccttcggcac caacgcgggc gacgtgcaga cgctcgcgca actcaacgcc
     1681 aagctcgccg cgctcgtcgg cgggaaagcc tcggtcaagg ccgcgaacgg cgatctgacc
     1741 atcgcggccg acaacctgac cgaccaagtc accgtgggcg ggacggccaa taccgcgaat
     1801 ttcggggtca acacgctgac cgccagtccg ccgacggtcg gaccggtgat cggcaacgac
     1861 gt
//
LOCUS       DATASH010000958         3001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3330738,
            whole genome shotgun sequence.
ACCESSION   DATASH010000958 DATASH010000000
VERSION     DATASH010000958.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3330738"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VHV99_25545"
     CDS             complement(<1..500)
                     /locus_tag="VHV99_25545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018417939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX3638784.1"
                     /translation="MKGVANVQDVLAIETRGQPADLPSSTYEMICQGAAINPVAPALS
                     FFATADDHRNAECWTYSELVRDVTRTANMFARLGVQRDSVVAYVLPNLPETHFVIWGG
                     EAAGIVCAINPLLESDAIGELLNASCASVLVTLAPFPGIDLWQKVQAVLHKLPCLRHL
                     VLVNPA"
     gene            complement(497..1453)
                     /locus_tag="VHV99_25550"
     CDS             complement(497..1453)
                     /locus_tag="VHV99_25550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006482290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX3638785.1"
                     /translation="MALHVLHYLHQDRAQWGVVANGAITPIPGEFRSTAEFIEANPVE
                     RLFLLSGPTIPESEVQWLSPVTANQQFICQGANYRQHMIESGMDPDVKKFNMIFTKAT
                     SCIVPADSPVVKPEGVQFLDYEIELGLVLRRDISSRETVTEENLHEYIAGAVIVNDYS
                     ARDIQIPQMQFYKGKSYRTFGPVGPYLCLLEKQDIAKLKELVLTLTVNGTVRQNDSTA
                     GLVYGPAETLTELSGIHDLRAGDLLATGTPSGCALSIPSPEKQRAAAQLPEAEKWSLF
                     LKMQEQRPQYLRVGDVVEAHIASYDRAINLGMQRNVVMEPAA"
     gene            complement(1488..2558)
                     /locus_tag="VHV99_25555"
     CDS             complement(1488..2558)
                     /locus_tag="VHV99_25555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017335048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3638786.1"
                     /translation="MKLTTAQPARHPDPTTKASALAYLIFERPDLGKAEQFLNDFGLQ
                     TVSRGEALLLLRGTGASHFCYVVRKAPKFRFVGFGLQVDSRAALDALAMLPGASEAER
                     SPLPGGGYIVQLTDPSGFRVDAIWGQAPASTLSHRPPLPFNSVDETVRINGTQRSPER
                     APEVIRLGHVVLELADYQETCAWYAQHFGLIPSDVQVLPDGSPVVAFMRLDLGDKPAD
                     HHTLALAQGFLPTYSHSAYEVVDADAVGMGQRILREKGWTHAWGIGRHILGSQIFDYW
                     QDPWGDKHEHYCDGDLFTADAPTGVHGVSREAMAQWGPTMPRSFTKPRFTPASIVALF
                     RNVRRSPDLTLSKLRTLAKLFA"
     gene            complement(2568..>3001)
                     /locus_tag="VHV99_25560"
     CDS             complement(2568..>3001)
                     /locus_tag="VHV99_25560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006410181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-(3-hydroxyphenyl)propionate hydroxylase"
                     /protein_id="HEX3638787.1"
                     /translation="STKLVRGAVIAQGWVRGSDGTTRLSDDVLGQGLALIGFGVDAAA
                     ALEHATAAAFARAGGRVVQIAHRGQRLHLAPRDSWEDLEGVFLPGLVPLGWAAVVRPD
                     KTIVHDGPATDANRIVRESLALLGNPLSVRTPEVETAIRTA"
BASE COUNT          589 a          919 c          924 g          569 t
ORIGIN      
        1 tcggccggat tcacgagcac cagatgccgc aggcaaggga gcttgtgcag caccgcttgc
       61 accttctgcc aaaggtcaat tccgggaaac ggcgccaggg tcacgagcac actcgcgcag
      121 gaagcgttga gcagttcacc gatggcgtca ctctcaagca gcggattgat cgcgcagaca
      181 atcccggcgg cttcaccacc ccagatcacg aaatgcgttt cagggagatt gggcagaacg
      241 tatgcgacaa ccgagtcgcg ctgtactccc aggcgcgcaa acatgttcgc ggtgcgcgtg
      301 acatcgcgca cgagctcgct atacgtccag cactcagcgt tccggtggtc gtcggccgtg
      361 gcgaagaacg aaagtgcggg tgccaccgga ttgatggcgg cgccctgaca gatcatctcg
      421 taggtactcg acggaagatc cgccggctgc ccccgcgttt caatagcgag gacatcctgt
      481 acgtttgcga cgcctttcat gccgccggtt ccataacgac gttgcgctgc atgcccagat
      541 tgatcgcgcg gtcatagctg gcaatatgtg cctccacgac gtcacccacg cgcagatact
      601 gcggcctttg ctcctgcatc ttcaggaaca gactccattt ctccgcttca ggcagctgcg
      661 ccgcggcgcg ttgcttctcg ggtgacggaa tgctcagtgc gcagccggaa ggcgtgccgg
      721 tcgcgagcag gtccccggcg cgcaggtcgt gtatgcctga gagctccgtc aaggtctctg
      781 ccggaccata gaccaggccc gccgtggagt cattctgccg gaccgttccg tttaccgtca
      841 gcgtcagcac gagctccttc agctttgcaa tatcctgttt ctccaggagg cagaggtacg
      901 ggccgacagg gccgaacgtg cgatagctct tgcccttgta aaactgcatt tgcggaatct
      961 ggatatcgcg cgcggaatag tcgttgacga tcacggcgcc ggcgatgtac tcatgcaggt
     1021 tctcctcggt gacagtttcg cgggacgaaa tgtcgcgtct gaggacgaga ccgagttcga
     1081 tctcgtagtc gaggaactgc actccctctg gtttaaccac gggcgagtcg gccggaacga
     1141 tgcagctcgt cgccttcgtg aagatcatgt tgaatttctt cacatccggg tccatgccgg
     1201 actcgatcat atgctggcga taattcgcgc cctggcagat gaactgctga ttggcggtga
     1261 cgggcgacaa ccattgcact tccgattcgg gaatcgtcgg gccgctcagc aggaaaagcc
     1321 gctcgaccgg gttagcctcg ataaactcgg cggtcgacct gaattcgccg ggaatcggcg
     1381 tgatagcgcc attagcgacc acgccccact gggcgcggtc ttgatgcaaa taatgcagaa
     1441 cgtgaagtgc catttatcga tctccggatt ctatggaagg ggttggttca ggcgaaaagc
     1501 tttgccagcg tgcgcagctt gctcagcgtc aggtcggggc tgcggcgcac gttcctgaag
     1561 agcgccacga tgctcgcagg cgtgaatctg ggcttggtga aactgcgcgg catcgtcggt
     1621 ccccattgcg ccatcgcctc acggctcaca ccatggacac cggtcggtgc atcggccgta
     1681 aagagatcgc cgtcgcagta gtgctcgtgc ttgtcgcccc atgggtcctg ccagtagtcg
     1741 aagatctggc tgcccagaat atggcggccg atcccccacg cgtgcgtcca gcccttctcg
     1801 cgcaggatgc gctggcccat gccaaccgcg tcggcgtcaa cgacttcgta ggcgctatgg
     1861 ctgtaggtgg gaaggaagcc ttgtgcaagc gcgagcgtat ggtgatcggc cggcttgtca
     1921 ccaagatcga gccgcatgaa ggcgacgacg ggcgagccat cgggaagcac ctgcacatcg
     1981 ctcgggatca agccgaaatg ctgcgcatac catgcgcagg tttcctgata gtcggcgagt
     2041 tcgagcacga catgcccgag ccggataact tcgggcgctc gctcgggcga tcgctgcgtc
     2101 ccgttaatcc ggacggtctc atccacagag ttgaacggca acggcggcct atgggatagc
     2161 gtcgaagctg gcgcctggcc ccatattgca tcgacgcgaa agccggaagg gtcggttaac
     2221 tgaacgatat agccgccgcc gggcaagggc gaccgttcgg cctcggaggc gcctggcagc
     2281 atggcaagtg catcaagagc ggccctgctg tcgacctgca gcccaaaacc gacgaagcgg
     2341 aatttcggcg ccttgcgcac gacatagcag aaatgcgaag cacccgttcc ccgcagaagc
     2401 aatagcgctt caccgcgcga tacggtctgt agcccgaaat cattgagaaa ttgctctgct
     2461 ttcccaaggt ctggccgctc gaatatcaga taggctaatg ccgacgcttt cgtcgtggga
     2521 tcaggatgac gggccggctg tgcagtggtc agtttcataa cggataatca ggcggttcga
     2581 atagcggttt cgacttccgg agtccgcacg ctcaagggat tacccagcag ggcgagactc
     2641 tcgcgcacga tacggtttgc atcggtggcc gggccgtcgt ggacgatcgt cttgtcaggc
     2701 cgcactacgg cagcccatcc gaggggcacc agcccgggaa gaaatacgcc ttcgaggtct
     2761 tcccagctgt cgcgcggggc aagatgcagt cgttgccccc gatgggcaat ctggacaacg
     2821 cgccctccgg cacgcgcaaa cgcggcggcc gtcgcgtgct cgagcgcagc cgccgcgtcg
     2881 acgccaaatc cgatcagcgc gagaccctgc ccgagcacgt cgtcgctcag acgcgtggtg
     2941 ccatccgaac ctctcaccca gccttgcgcg atgactgcgc cacgaacaag cttcgtcgac
     3001 g
//
LOCUS       DATASH010000959         2768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2341672,
            whole genome shotgun sequence.
ACCESSION   DATASH010000959 DATASH010000000
VERSION     DATASH010000959.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2341672"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..305)
                     /gene="rpsT"
                     /locus_tag="VHV99_25565"
     CDS             complement(27..305)
                     /gene="rpsT"
                     /locus_tag="VHV99_25565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEX3638788.1"
                     /translation="MANSAQARKRARQAAKANSHNSALRSKFRTAIKAVRKAIDAGDK
                     AKAAEIFLASSKTIDIIADKKIVHKNKAARHKSRLAAAIKGLQASAAQ"
     gene            810..2360
                     /gene="murJ"
                     /locus_tag="VHV99_25570"
     CDS             810..2360
                     /gene="murJ"
                     /locus_tag="VHV99_25570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEX3638789.1"
                     /translation="MNLIRALLTVSGFTLLSRVTGLARETLIARAFGASQYTDAFYVA
                     FRIPNLLRRISAEGAFSQAFVPILAEFKNQQGHDATKALVDATSTVLAWALAVLSVIG
                     VVGASGVVFVVASGLAHEGHAYQLAVAMTRIMFPYIIFISLTSLASGVLNTYKNFSLP
                     AFAPVLLNVAFIFAAVFLAPRLQTPVYALAWAVIVGGALQFVVQLPGLKKIDMVPRIG
                     LSPLRALAHRGVKRVLAKMVPAMFAVSVAQISLIINTNIASHIGPGAVSWINYADRLM
                     EFPTALLGVALGTILLPSLSKAHVDADSHEYSSLLDWWLRITFLLAAPSAIALFFFAQ
                     PLTAVLFNYGKFDGNSVVMVSRALAAYGVGLVGLILIKILAPGFYAKQDIKTPVKIGI
                     GVLVVTQLSNYVFVPVFAHAGLTLSVGLGACVNALLLFIGLRKRGIYMPSSGWLKFFV
                     QLLGACLVLAGVMRWLAISFDWIGMHSQPVSRIVLLGACLVLFAALYFGMLWLMGFKY
                     AYFKRRVK"
     gene            2366..>2768
                     /locus_tag="VHV99_25575"
     CDS             2366..>2768
                     /locus_tag="VHV99_25575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEX3638790.1"
                     /translation="MSRVLDYFSTLVAEDESLPLTETALSIAQDAYPDLDLQSVLAEI
                     DELVLRLRRRIPDDANVRQKVVILNRFFFRELGFASNLNDYYDPDNSHLNAVLKRRRG
                     IPISLAVLYLEMAEQIGIPVRGVSFPGHFLLR"
BASE COUNT          456 a          859 c          853 g          600 t
ORIGIN      
        1 cgcccaccga agcgggctga ccgcaattac tgggctgcgg acgcctgcag acccttgatg
       61 gctgcagcca gacggctctt atggcgagca gccttgttct tatgaacgat tttcttgtcg
      121 gcgatgatgt cgatggtctt cgacgatgcg aggaagattt cagcggcctt ggccttgtcg
      181 ccggcgtcga tcgccttgcg cacagccttg atggccgtgc ggaacttcga gcgcagtgcc
      241 gagttgtgcg agtttgcctt ggcggcctgg cgggcgcgct tgcgtgcttg tgcggagtta
      301 gccatgacgg ttccttatcc tgttcctgtt tccagtaccc ggccttcaat gttgaggcga
      361 ggtgctgctt ctcgatccga acccctggaa gcgattgccc aagggcgcaa aagtgtcgaa
      421 aaatcgatcg aactacgcga aggcgggccc atgttttcag gtattcagat gcattgacag
      481 acggcgctgc gccaacctga gaatcccgtc cgaagaccat tcgaaaactg agcaagcttc
      541 gaaaccgacg attatagcaa caaaatcagg cacgtgacaa cggaaaacgc gcgccgccgc
      601 agtggccagt cacgcgtcgc tttggcgttt atgcgctggt cctcgtgcaa gggcgccggc
      661 ggaaagtgcc gacggtaccg gcacggccgc aaaagacgca aaaatttgca ctttccggcc
      721 gatcgagacg aacgtccgca cggcaaatcg gtccgaatcg cgttccggct cgtcgcccgc
      781 aatggcggga cccgtataat aagcgcccca tgaatctaat ccgagccctg ctgacggtca
      841 gcggcttcac gctgctgtcg cgcgtgaccg gactggcccg tgaaacgttg atcgcccgtg
      901 cgttcggcgc cagtcaatac accgacgcgt tctacgtcgc ctttcgcatt ccgaacctgc
      961 tgcgccgcat ttccgccgaa ggcgcgttct cgcaggcctt cgtgccgatc ctcgccgagt
     1021 tcaagaacca gcaaggccac gacgccacca aggcgctcgt cgacgcgacc tcgaccgtgc
     1081 tcgcgtgggc tctcgccgtg ctgtcggtga tcggcgtggt cggcgcttcg ggcgtcgtgt
     1141 tcgtagtcgc gtctggtctc gcccacgagg ggcacgcgta ccagcttgcg gtcgcgatga
     1201 cgcgcatcat gttcccgtac atcattttca tctcgctgac gtcgctggcg tcgggcgtgc
     1261 tgaatacgta caagaacttc tcgctgcccg cgttcgcgcc cgtgttgctg aacgtcgcgt
     1321 tcatcttcgc ggcggtgttc ctcgcgccgc gcctgcaaac gccggtctat gcgctcgcat
     1381 gggcggtgat cgtcggcggc gcgctgcaat tcgtggtgca gctgccgggc ctcaaaaaga
     1441 tcgacatggt gccgcgcatc ggcctgagtc cgctgcgcgc gctcgcgcat cgcggcgtga
     1501 agcgcgtact cgcgaagatg gtgccggcga tgttcgcggt gtcggtcgcg cagatcagcc
     1561 tgatcatcaa cacgaacatc gcgtcgcaca tcgggccggg cgcggtgtcg tggatcaact
     1621 acgccgaccg gctgatggag tttccgaccg cgctgctcgg cgtcgcgctc ggcacgatcc
     1681 tgttgccaag cctgtcgaag gcgcacgtcg atgccgattc gcacgagtat tcgtcgctgc
     1741 tcgactggtg gctgcgcatc acgttcctgc tcgccgcgcc aagcgcgatc gcgctgttct
     1801 tcttcgcgca accgctgacg gccgtgctgt tcaactacgg caagttcgac ggcaactcgg
     1861 tcgtgatggt gagccgcgcg cttgccgcct acggcgtcgg tctggtcggc ctgatcctga
     1921 tcaaaatcct cgcgccgggc ttctacgcga agcaggacat caagacgccg gtgaagatcg
     1981 gcatcggcgt gctggtcgtc acgcagctga gcaactacgt gttcgtaccg gtgttcgcgc
     2041 atgcgggtct tacgctgagc gtcgggctcg gcgcttgcgt caatgcgttg ctgctgttca
     2101 ttggtctgcg caagcgcggc atctatatgc cgtcgagcgg ctggctcaag ttcttcgtgc
     2161 agttgctcgg cgcgtgcctc gtgctcgccg gcgtaatgcg ctggcttgcg atcagcttcg
     2221 actggattgg catgcatagc cagccggtca gccgcatcgt gttgcttggt gcgtgcctcg
     2281 tgctgttcgc cgcgctatat ttcggtatgc tttggctgat gggcttcaag tacgcgtatt
     2341 tcaaaaggcg agtgaagtga tcaccatgtc gcgggttctc gattatttca gtacgctcgt
     2401 cgccgaagac gagagcctgc cgctgaccga gacggcgctc tcgatcgcgc aggacgccta
     2461 tcccgacctc gatctgcagt cggtgctcgc cgagatcgac gagctagtgc tgcggctgcg
     2521 gcgccgcata cccgacgatg ccaacgtccg tcagaaggtt gtcattctca atcgtttctt
     2581 tttccgcgaa ctcggtttcg cgagcaatct gaacgattac tacgaccccg acaacagcca
     2641 tctgaatgcc gtgctcaagc gccgccgcgg cattccgatt tcgcttgcgg tgctgtatct
     2701 ggagatggcc gagcaaatcg gcattccagt gcgcggcgtg tcgtttcccg gtcatttcct
     2761 gctgcgcg
//
LOCUS       DATASH010000960         3395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2735724,
            whole genome shotgun sequence.
ACCESSION   DATASH010000960 DATASH010000000
VERSION     DATASH010000960.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2735724"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="VHV99_25580"
     CDS             complement(<1..965)
                     /locus_tag="VHV99_25580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007744964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HEX3638791.1"
                     /translation="MRACPALFISAPASNQGKTTVTAGLARYHARQGLRVRVFKTGPD
                     FLDPMILERASGAPVYSLHLWMVGESGCRALLAKAAQEADLILIEGVMGLFDGTPSSA
                     DLATTFGVPVLAVIGAHGMAQTFGAMALGLARYRADLPFYGVLANRVASARHAQMLSE
                     AIPEGIRYCGHLPSTDDIALPGRHLGLTQAAEVTDIDARLDRAADAIGETTLRELPPA
                     VMFADEALEQPPRLLEGMHIAIARDAAFSFIYPANLDLLTAMGATLAPFSPLADEPVP
                     AHADALYLPGGYPELHAARLAANAVSAASIRAHVEAGKAIVAECGG"
     gene            complement(967..1569)
                     /gene="cobO"
                     /locus_tag="VHV99_25585"
     CDS             complement(967..1569)
                     /gene="cobO"
                     /locus_tag="VHV99_25585"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX3638792.1"
                     /translation="MKTDEESHLRMTQRRKEGHEKKQAAAALEKGLLIVNTGTGKGKT
                     TAAFGMAVRVLGHGMRLGVVQFIKGALHTSERDFLGAIAHCDFITMGDGYTWDTQNRD
                     ADIATARKGWDAARTMIESGEYQMVVLDELCTVLKYEYLPLDEVLTVLTARPEMLHVV
                     VTGRHAPEALIEAADLVTEMRAIKHPYREQGVKAQRGVEF"
     gene            complement(1566..1976)
                     /locus_tag="VHV99_25590"
     CDS             complement(1566..1976)
                     /locus_tag="VHV99_25590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007744951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HEX3638793.1"
                     /translation="MSAWVIGIGCRRGVSVEQIHAAVFAALGTRPLASARALASIDTK
                     KDETALIEFAARHSLPLRFFSKQRIAQVETGTSERVQALLGIDGVCEPCALLASHNGR
                     IIVPKTATDGVTVAIAEDDLKSPIRQPTDNERTS"
     regulatory      complement(2140..2477)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     regulatory      2638..2918
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3100..>3395
                     /locus_tag="VHV99_25595"
     CDS             3100..>3395
                     /locus_tag="VHV99_25595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014897050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX3638794.1"
                     /translation="MQMRKIPVTVVTGFLGSGKTTLMRHILQNAKGLRIAVIVNEFGE
                     LGIDGEILKGCNIGCEDESATDQPNLYELSNGCLCCAQLNGKQGALPDPPVVPT"
BASE COUNT          636 a         1043 c         1065 g          651 t
ORIGIN      
        1 ccgccgcatt ccgcgacgat tgctttaccc gcttccacat gcgcgcgaat cgaggcggca
       61 ctcactgcgt tcgctgcaag ccgggcggcg tgcaattccg gatatccgcc cggcagataa
      121 agtgcgtcgg catgcgcggg aacgggttca tcggcgagcg gtgagaaggg cgcgagcgtt
      181 gcccccatcg ccgtcagcag atcgagattg gccgggtaga tgaacgaaaa cgcggcatcg
      241 cgggcaatgg cgatgtgcat gccctcgagc aaacgcggag gctgttcaag ggcttcgtcg
      301 gcgaacatta ccgcgggcgg caactcgcgc aacgtcgtct cgccaatggc atccgctgcg
      361 cgatccagcc gcgcatcgat gtccgtcacc tctgctgctt gcgtgagacc gaggtgtcgc
      421 ccgggcagcg caatgtcgtc ggtgctcggc aaatgaccgc aatatcgaat gccttcagga
      481 atcgcttcgc tcagcatttg cgcatgccgt gccgacgcca cgcgattggc gagcacgcca
      541 taaaacggca ggtccgcgcg atagcgcgcg agtccgagcg ccattgcgcc gaaggtctgc
      601 gccatgccgt gcgccccgat cacggcgagt accggcacac cgaacgtggt ggcgagatcg
      661 gcgctgctgg gggtgccgtc gaagagaccc atcacgcctt cgatcaggat caaatcggcc
      721 tcctgcgcgg ctttcgcgag cagcgcgcgg cagccgcttt caccgaccat ccacaaatgc
      781 agcgaataca ccggcgcgcc ggacgcgcgt tccaggatca tcggatcgag gaagtcgggg
      841 ccggtcttga agacccgcac tcgcagacct tgcctcgcgt gatagcgcgc gagcccggcg
      901 gtgacggtgg tcttgccctg gttcgatgcg ggtgcgctga tgaataaagc ggggcaggcg
      961 cgcacgtcag aactcaacgc cgcgttgcgc cttgacccct tgctcgcgat acggatgctt
     1021 gatcgcgcgc atctccgtca caagatcggc tgcttcaatc agcgcctcgg gcgcatggcg
     1081 gcccgtcacg accacatgca gcatctcggg ccgggcggtg agcaccgtga gcacttcgtc
     1141 gagcggcagg tactcgtatt tgagcaccgt gcacaattca tcgagtacga ccatttgata
     1201 ctcaccgctt tcgatcatcg tgcgcgcggc gtcccagccc ttgcgagccg tggcgatatc
     1261 ggcatcgcga ttctgcgtgt cccacgtgta gccgtcgccc atggtgatga aatcgcagtg
     1321 cgcgatcgcg ccaaggaaat cgcgttcgga tgtatgcaac gcgcccttga tgaactgcac
     1381 tacgccgagc cgcatgccat gcccgagcac acgtacggcc atgccgaacg ccgcggtggt
     1441 cttccccttg cccgtgcccg tattgacgat caggagaccc ttctccagcg ctgcggcggc
     1501 ctgctttttc tcgtggcctt ccttgcgccg ctgagtcatg cgcagatgcg attcttcgtc
     1561 cgttttcatg atgttctctc gttgtcggtc ggttgcctga ttggtgactt aaggtcgtct
     1621 tcagcgatag cgacggtcac gccatcggtc gctgttttcg gcacgatgat gcgtccgttg
     1681 tgcgatgcca gcagcgcgca gggttcgcag acaccgtcga tgccgagcaa cgcctgaacg
     1741 cgttccgacg tgccggtttc aacctgcgcg atgcgttgct tcgaaaaaaa acgcagcggc
     1801 aaactgtggc gagcggcgaa ttcgatcagt gcggtttcgt ctttcttggt atcaatcgat
     1861 gccagcgcgc gagcgctcgc cagcggccgc gtccccagcg ccgcgaacac ggcagcatgg
     1921 atctgttcga cgcttacgcc gcgccggcag cctatgccga tgacccatgc gctcatgatt
     1981 gcgcccggtt cgcgcgacct ggaaacgcgc tggttagccg gtggaacgat cgacgcgagg
     2041 gcggacgaaa gcgctgaaaa attggattca tgtcagtcga taaaagcatt gcgtccttca
     2101 tccgtccctc gcggatgtgg cgtccggcgt gcttttagag cacgccactt ggcgactggc
     2161 cggtatccgg gctgggcgac cgcattccgc tcgccttccc gctcgtcgga gcagtggctt
     2221 tggatgaaat gggcgcgcgg ttcgaatgag agtcatgggt gaaagtcacg agtgaaagtc
     2281 acgaacgaga gtcagtcgac gcgcgcgggt cgcttaccgt tgcggggaca gcacaggttt
     2341 tgtcgacacg gcgcgcgagg cctgaagcgt cagtcttgag cgttgggcct tgaaaatacg
     2401 cgcgtgtgca tcctgtttcc cgtttaacta cgcgcgccgg aaaagcgcgc gtgagcacca
     2461 gacgcgggcc aaagatagca cagcgggtgt tggttgcgct gggcttttag aggcaggaac
     2521 cgaccggcag cacaggtttt ttgcgctcgc agcggtatga gatctctcca ggagccgcgg
     2581 cccgaaccct cgcccacgct ccgctcaaac cttgacgcta atcccccatc ggcctagact
     2641 tcgcggtagt tttctggtgc tcgcgcgcgg tctctgctgc gcgcagttaa acgggaagca
     2701 gggagcgttg ccggatcccc cggtcgtgcc aacctgcgct gcccccgcaa cggtcaagcg
     2761 aaaatcgcgc gacgtggtct taccctgatt cacgccgggt tcatgccatt tcgcgcggta
     2821 aagccggctt cgaaaaacca ctgcatggct atgtcatgcg ggaaggtgaa gcggcatttt
     2881 cgccagcccg gataccggcc ggaacgagtc aacacctgaa gcggacgatg tcgcggggat
     2941 gcggcatacg tttgacactt ttcactgtgc tgttccggat tgccatcctg catcgcgcac
     3001 tcgctgcgac acccgcgtgt gcattttact gctcgtacga ctcagcaaag ggccaggcat
     3061 tccggcagca agcagccaat cgatactacg gagcaacgga tgcaaatgcg caaaatcccg
     3121 gtcacggtcg taacgggttt cctcggcagc ggcaagacca cgctgatgcg gcacatcctg
     3181 caaaacgcga aagggctgcg gatcgcggtg atcgtgaacg agttcggcga actgggcatc
     3241 gatggcgaaa tcctgaaggg ctgcaacatc ggatgcgagg atgaaagcgc aacggatcag
     3301 ccgaatctgt atgagttgtc aaacgggtgt ctctgctgcg cgcagttaaa cgggaagcag
     3361 ggagcgttgc cggatccccc ggtcgtgcca acctg
//
LOCUS       DATASH010000961         8334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5110657,
            whole genome shotgun sequence.
ACCESSION   DATASH010000961 DATASH010000000
VERSION     DATASH010000961.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 8334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8334
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5110657"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="VHV99_25600"
     CDS             complement(<1..433)
                     /locus_tag="VHV99_25600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3638795.1"
                     /translation="MDLRHLPSLNAIKAFEAAARHESFSRAADELFVTHGAVSHQIRA
                     LEAELGITLFARDGKRVRLTDTGRRYAMRVRTALMALADATREIRAGDRERRLVVSML
                     TSFAARWVTPRIGSFIEAHPQWDLELLSTNALTDFARDDVDV"
     gene            532..858
                     /locus_tag="VHV99_25605"
     CDS             532..858
                     /locus_tag="VHV99_25605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015001499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2917 domain-containing protein"
                     /protein_id="HEX3638796.1"
                     /translation="MREISSSIAFEIRSGETVPMKVARSTKLVVRGGAVWVTRSDDTE
                     DYWLQPGHTLRLRRGERLWLSAEGNTQAKVAFSVPTRCDERALNWFARVGERLAARMR
                     GGWRTV"
     gene            1064..1789
                     /gene="lipB"
                     /locus_tag="VHV99_25610"
     CDS             1064..1789
                     /gene="lipB"
                     /locus_tag="VHV99_25610"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEX3638797.1"
                     /translation="MCAAPVSPSPLPATAPLTLRWLGSELYEASFESMRAFTDARTAD
                     TPDEIWLVEHPPVFTLGQAGDPAHLLAADSGIPLVKVDRGGQITYHGPGQVVAYLLLD
                     LRRRKLMVREMVTRIEQAVIDTLAAYNLAGERKAGAPGIYVAPGPHAGLHAGAKIAAL
                     GLKIRNGCSYHGVSLNVNMDLRPFLAINPCGYAGLETVDMATLGVAAGWDHVARTFAA
                     RLTANLDGSPAAVAQPQAGALTA"
     gene            1803..2810
                     /gene="lipA"
                     /locus_tag="VHV99_25615"
     CDS             1803..2810
                     /gene="lipA"
                     /locus_tag="VHV99_25615"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015601526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX3638798.1"
                     /translation="MTDVTANLAASPAPDAVPASAAAYDATAKQKAQAKTARIPIKIV
                     PIEKLKKPDWIRVKAATGNSRFYEIKQILREHNLHTVCEEASCPNIGECFGKGTATFM
                     IMGDKCTRRCPFCDVGHGRPDPLDVDEPGNLARTIAALKLKYVVITSVDRDDLRDGGA
                     AHFVECIRQTRELSPETRIEILTPDFRGRLDRAIGILNAAPPDVMNHNLETVPRLYKE
                     ARPGSDYAHSLKLLKDFKALHPEVATKSGLMVGLGETEEEILQVMRDLREHDVDMLTI
                     GQYLQPSEHHLPVRSYVHPDTFKMYEEEAYKMGFTHAAVGAMVRSSYHADLQAHGAGV
                     V"
     gene            complement(2928..3062)
                     /locus_tag="VHV99_25620"
     CDS             complement(2928..3062)
                     /locus_tag="VHV99_25620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638799.1"
                     /translation="MPNLIDRLIGDDAFRDRFILFLYPFTIIGGIISVTCSLLAHHYR
                     "
     gene            complement(3119..3610)
                     /locus_tag="VHV99_25625"
     CDS             complement(3119..3610)
                     /locus_tag="VHV99_25625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638800.1"
                     /translation="MIGGLPSIRIPMIDFLSRVSWLFFNVGVPVFAPIALLPLLNFSR
                     HYRHVAQDITRRSIQDGQLFWVVISMCASGCYEIGCALSEAPTRSTLALMLAALLWHA
                     LFIVVASILVCLGAADAAARSTTRAARHAREQRRGGRLMWFSLLVTTVVAASFSASHY
                     SFT"
     gene            3866..4348
                     /locus_tag="VHV99_25630"
                     /pseudo
     CDS             3866..4348
                     /locus_tag="VHV99_25630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493272.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            4466..6133
                     /locus_tag="VHV99_25635"
     CDS             4466..6133
                     /locus_tag="VHV99_25635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEX3638801.1"
                     /translation="MLILLNLLAGVALLVWGSHIVRTGILRVLGADLRSVLGRSTSSR
                     WRAFLAGVGVTSLVQSSNATAVIVTSFAAQGLLPLTSGLAIMLGADVGTALMARVLTL
                     DLSWLSPILILFGVPLFLSRKQTRLGQVGRTLLGLGLILLALHLIVEAAQPMMQGAGV
                     RVMFGALTGDTMLDALVGAAFAVLSYSSLAAVLLTATLASSGVISLKVALCLVIGANL
                     GSGLLALMGTVAQNAAARRLALGSLAFKLAGALLILPFAPLLARGLPVLIANPREAVV
                     GFHVIYNTLRCCACLSLIDPVARICTRVLPDRPLPNGELRPLHLDAAALSTPTLALAH
                     AAREVLRIGDIVRTMLDNVAELIHQNSIEKARETVRMDDDVDELYAAVKSYLTLISRE
                     QLDEADSRRWTDIIALTINLEHAGDIIERIVGDIEEKKIAHRLSFSEEGLGELDDLQA
                     RLVSNLQLGLSVFLNNDLRCAERLLAEKERFRDLERAYSYRHLDRLAGQTLRSIETSA
                     LHLDLISDMKRLNSLFCSTAYPVLDAAGALHDTRLRKRVLDTMHVKE"
     gene            complement(6148..6918)
                     /locus_tag="VHV99_25640"
     CDS             complement(6148..6918)
                     /locus_tag="VHV99_25640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEX3638802.1"
                     /translation="MWQEDRHQRIRALLSTLQRVSTERIMADLGVSRETVRRDLLDLE
                     ALGELRRVHGGAIKPAEEAPIAERAHMRVKAKTAIAKAATRLIASGQTLFIDAGTTTA
                     ALTEELAKLANLTIVTNSIDVALKMRGAVDQTEAANEVILLGGSISDRAMATTGATTV
                     LDIQRYRADMALLSPVGIDHRHGATNYDHAETEVARAMVANADRVVILADYSKIGQRS
                     RISYCPVDRIDVLVTNKKAAEAADFASLKKKLEEIVLA"
     gene            7494..8258
                     /gene="ugpQ"
                     /locus_tag="VHV99_25645"
     CDS             7494..8258
                     /gene="ugpQ"
                     /locus_tag="VHV99_25645"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEX3638803.1"
                     /translation="MGNGIDRTLSADWPYPKLVAHRCGGTLAPENTLAGFDACVRYGY
                     RMVEFDAKLSADNQLFLLHDDTLERTTNGQGAAAEHTWKQLAALDAGAWFGPQFAGTH
                     LPTLAKAAGRCAQDGLAANIEIKPCPGRDEITGRLVASGALTLWQGQTPPLLSSFSFE
                     ALAAARDAAPLLQRGMLFEEVPADWLRIVRELDCVSLHASHRYLTEPLVAEIKATGLR
                     VLAYTVNDPERARLLAQWGVDMICTDRIDRLEHAMF"
BASE COUNT         1593 a         2523 c         2641 g         1577 t
ORIGIN      
        1 ccacgtcgac gtcgtcgcgt gcgaagtccg ttagcgcgtt ggtggacagc aattcgaggt
       61 cccattgtgg atgcgcctcg ataaagctgc cgatacgtgg cgtcacccaa cgcgccgcga
      121 acgatgtgag catggaaacg accagccgcc gttcgcggtc gccggcgcgg atttcgcgcg
      181 tggcgtcggc gagcgccatg agcgcggtgc gcacgcgcat ggcatagcgg cggccggtat
      241 ccgtgagccg tacgcgcttg ccgtcgcggg cgaacagcgt gatgcccaac tccgcttcca
      301 gcgcgcgaat ctgatggctg acggcgccgt gcgtgacgaa cagttcgtcc gcggcgcgcg
      361 agaagctctc gtggcgggcg gcggcttcga atgccttgat tgcattcaat gaggggaggt
      421 gccggaggtc catcgcaata tttctcacat agaggtgaaa atagatcgtt ttgcgtaaac
      481 ccttgctgag cctaggattg tagtcatgaa acgaactttg gcgagggcat catgcgagaa
      541 atttcctcta gtatcgcttt tgaaatccgc agcggcgaga ccgttcccat gaaggtagca
      601 cgcagcacca agctcgttgt gcgtggcggc gcggtgtggg taacgcgcag cgacgacacg
      661 gaagactact ggctgcagcc cggtcacacg ctgcgcttgc gccgcggcga acgcttgtgg
      721 ttgagcgccg agggcaacac acaggcgaag gtcgcgtttt ccgtgccgac ccgctgtgac
      781 gaacgtgcgc tgaactggtt cgcacgggtc ggcgaacggc tggcggcccg gatgcgtggg
      841 ggctggcgga cggtctgaat ccagcaggcg ccgccgtgct gcggatgagc gcagttgaaa
      901 ggcgctggag atgagagcgg ccctccgggc ggaacagcgt ggcattggca agatgccacg
      961 ctgcaccaat gcgatgctct ggcgtgaagc cgcagcgacg ctgatgcgcg gatgccagtc
     1021 acatccggcg gggattcaca gatttcggta aactattggg atcatgtgcg ccgccccggt
     1081 ttccccgtct cccttgcccg ccacggcgcc gctcacgctg cgctggcttg gcagcgaact
     1141 ctacgaagcc agtttcgaat caatgcgcgc gttcaccgac gcgcgcactg ccgatacacc
     1201 agacgaaatc tggctggtcg aacacccccc cgtcttcacg ctcggccagg ccggcgaccc
     1261 ggcgcatcta ttggccgccg atagcggtat tccacttgtc aaggtcgacc ggggcgggca
     1321 gatcacctac cacggccccg ggcaggtggt tgcctacctg ctgctggacc tgcgccgccg
     1381 caagctgatg gtgcgcgaga tggtcacgcg gatcgagcag gccgtgatcg ataccctcgc
     1441 ggcgtataat ctcgccggag aacgcaaggc gggcgcccct ggcatctacg tggcgcccgg
     1501 gccgcacgcc gggctgcatg ccggcgccaa aatcgccgcg ctgggtctga aaatccgcaa
     1561 cggctgcagc tatcacggcg tgagcctgaa tgtgaacatg gacttgcggc cgtttctggc
     1621 catcaaccca tgcggctacg cagggctcga aacagtcgac atggcaacgc tgggcgttgc
     1681 ggccggctgg gaccacgtgg cccgtacctt tgccgcacgc ctcaccgcaa acctcgacgg
     1741 cagtcccgcg gccgtcgccc aaccgcaggc cggtgctctc accgcttgac tggaccgaac
     1801 gaatgactga cgttaccgcg aacctcgcag cttctcccgc tcctgatgcc gtgccggctt
     1861 ccgccgctgc ctacgacgct accgcgaagc agaaagcaca agccaaaacc gctcgtatcc
     1921 cgatcaagat tgtcccgatc gaaaagctga aaaagccgga ctggatccgc gtcaaagcgg
     1981 ccaccggcaa ttcgcgcttc tacgaaatca agcagatttt gcgcgagcac aacctgcaca
     2041 cggtgtgcga agaagcgagc tgcccgaata tcggcgaatg cttcggcaag ggcaccgcga
     2101 cgttcatgat catgggcgac aagtgcacgc gccgttgccc gttctgcgac gtcggccacg
     2161 gccgccccga tccgctcgac gtggatgagc cgggcaacct ggcccgcacc atcgccgcgc
     2221 tgaaattgaa gtacgtggtg atcacgagcg tcgatcgtga cgatctgcgt gacggtggcg
     2281 ccgctcactt cgtggaatgc atccgtcaga cgcgcgagct gtcgccggaa acacgcatcg
     2341 aaattctgac gccggacttc cgcggccgtc tggaccgcgc gatcggcatt ctgaacgccg
     2401 cgccgcccga tgtgatgaac cacaacctcg aaacagtacc gcgtttgtac aaggaagcgc
     2461 gccccggttc ggactacgcg cattcgctga agctgctgaa ggacttcaag gcgctgcatc
     2521 cggaggtcgc gaccaagtcg ggcctgatgg tcggtcttgg cgaaaccgag gaagaaatcc
     2581 tgcaagtgat gcgcgatctg cgcgagcacg acgtggacat gctgacgatc ggccagtatc
     2641 tgcaaccgtc ggaacaccat ctgccggtgc ggtcgtatgt gcatccggac acgttcaaga
     2701 tgtacgaaga agaggcctac aagatgggct tcacgcacgc agcggtgggc gcaatggttc
     2761 gctcaagcta ccacgctgat ctgcaggcgc atggagcagg ggtggtctga acacttaaat
     2821 gtgttgtgag tttgaaaatg caaagcccgc acgtgttgcg ggctttgttt tttggggcgc
     2881 gggagtgggc ggttcagcag ctccgctcgg cgatctgcct gcgccgccta gcggtaatgg
     2941 tgcgcgagca gactgcaagt aaccgatatg attccgccaa tgatcgtgaa cggatagaga
     3001 aagaggatga agcgatcgcg aaatgcgtcg tctccaatga ggcggtcgat caaattgggc
     3061 atgttgaatc tccttgtgag actagggtga aaagtatatc tcagtgcgtt tgaattagtt
     3121 aggtaaacga ataatgagat gcggaaaagg aggcggcaac caccgtggtt acgagcaagg
     3181 aaaaccacat caaccttccg ccgcgcctct gttctctcgc atgacgcgct gcacgagtcg
     3241 ttgaacgagc cgctgcatcg gccgcgccca gacacaccag tattgacgct acgacgataa
     3301 agagcgcgtg ccagagaagc gcggccagca tgagcgccaa cgtgctacgc gtcggcgctt
     3361 cgctcaaagc acaaccaatc tcgtagcacc cgctcgcaca catcgaaatg acgacccaga
     3421 aaagctgacc atcctggatg gatcgccgcg taatgtcttg cgccacgtgc cggtagtggc
     3481 gcgaaaaatt cagcagagga agtaatgcaa tcggcgcaaa aacaggcacg ccgacattga
     3541 aaaacaacca gctcacgcgg ctaagaaaat cgatcatagg aatgcgaatt gaaggcaagc
     3601 cgccgatcat gccgccgcca cggaagcacg acaatccgcc gaacggccag cttgcgctaa
     3661 ggcatcccaa gccgtcatca ttcgctggaa cgcacccact gcgaaccgca acagtcgcgc
     3721 cgatccagct cagcgtgaaa agcgattctt gagcagcgtc attagtaatc ctggattaat
     3781 tagaaccgcg cgcggacaaa ctccctatag tcgaaagcct cccagacaac acacactaaa
     3841 ggcggctgca tgtcgcctac gaaccgcgct cggcctgagc tttcagcgcg tgccgaaggc
     3901 gcagcgcgag cgcatcgcgt tggagatact gaacctggtg ggcctcgagc agcatgcgcg
     3961 ctcgcgggtc gacgcgctgt cgggtggcat gcagcagcgc gtgggagttg cacgcgcgtt
     4021 ggcgagcgat ccgcaagtgc tgctgatgga cgagccgatg ggcgcacttg acgcgatgac
     4081 gcgcgagtcg atgcaggagc ttgtactgga cgtctggggc cgcacgcgca agacggtgtt
     4141 tttcatcacg catagcgtgg aggaagcgct gtttctagcg acacggctgg tcgtgatgac
     4201 tctggggccg ggccgtatcg ccgagagcta cgacctgcca ttcgcgcggc gctttctcga
     4261 gacacgcgac gcgcgcggtg ccgtggaagg gacgggtgta gttgttgcgg ccaaggccgt
     4321 ccagtggttg cacgagcgcg tcgtttaaaa gcacaaactt acaatatggc aaaaatgtgc
     4381 aacactactg tcataaacat cccgggtgcc ggctcacgcg gcatgcggtt cgggtcatgg
     4441 cagcaggacg gatgcgcagt gccttatgtt gattctcctt aaccttctgg ccggtgtggc
     4501 gctcctcgtg tggggctcac acatcgtgcg taccggcatc ctgcgcgtgc tcggcgccga
     4561 cctgcgcagc gtcctcgggc gcagcaccag cagccgctgg cgtgccttcc tcgcgggcgt
     4621 cggcgtcacg agtctcgtgc agagcagcaa tgcaacggcg gtgatcgtca cgtcgttcgc
     4681 cgcacaaggt ctgctgccgc tcaccagcgg tctcgcgatc atgctcggcg ccgacgtcgg
     4741 caccgcgctc atggcgcggg tgctcacgct cgatctgtca tggctgtcgc cgatcctgat
     4801 tctgttcggc gtaccgcttt ttctgtcgcg caagcaaacc cgtctcggcc aggtcggccg
     4861 tacgctgctc gggctcggct tgattctgct ggcgctgcat ctgatcgtcg aagccgcgca
     4921 gccgatgatg caaggcgccg gcgtgcgcgt gatgttcggt gcgctgaccg gcgacaccat
     4981 gctcgacgcg ctagtcggtg cggcatttgc ggtgctgtcc tattcgagtc tcgcggcggt
     5041 cctcttgacg gcaacgcttg cttcgtccgg cgtgatttca ctgaaggtgg cgttgtgtct
     5101 cgtgatcggc gcgaacctcg gcagcggcct gctcgcgctg atgggcacgg tcgcgcagaa
     5161 cgcggcggcg cggcgcctcg ccttgggcag tctggcgttc aagctggccg gcgctttgct
     5221 gatcctgccg ttcgcgccgc tgcttgcacg cgggctgccg gtgctgatcg cgaatccgcg
     5281 cgaagcggtg gttggctttc acgtgatcta caacacgctg cgttgctgcg catgcctttc
     5341 gctgatcgat ccggtggcgc gcatctgtac gcgggtgttg cccgaccggc cgctgccgaa
     5401 cggcgaactg cggccgctgc atctggatgc cgcagcgctt tccacgccca cgctcgcact
     5461 cgcgcatgcc gcacgggagg tgctgcgcat cggcgacatc gtgcgcacca tgctcgataa
     5521 cgtcgcggaa ctgattcatc aaaacagcat cgagaaggcg cgcgaaacgg tccgtatgga
     5581 cgacgacgtg gacgagctct acgccgcggt caaaagctat ctgacgctga tctctcgcga
     5641 gcaactcgac gaagccgaca gccggcgctg gaccgacatc atcgcgttga cgatcaatct
     5701 ggagcacgcg ggcgacatca tcgaacggat cgtcggcgat atcgaggaaa agaagatcgc
     5761 gcaccggttg tcgttttcgg aggaagggct aggcgaactg gacgatctgc aggcgaggct
     5821 cgtcagcaat ctgcagcttg ggctgtcggt gtttctgaac aacgatctgc ggtgtgccga
     5881 gcggctgctg gcggagaagg agcgctttcg cgacctggag cgcgcgtatt catacaggca
     5941 cctggaccgg cttgccgggc aaaccttgcg cagcatcgaa accagtgcgt tgcatctgga
     6001 tctgatcagc gacatgaagc ggctcaattc actgttttgt tcaaccgcct atccggtgct
     6061 cgacgcagcc ggcgcgttgc atgacacgcg cctgcgcaag cgtgtgctcg atacgatgca
     6121 tgtgaaggag taggccgagg cctacgccta cgccagaacg atctcttcca gcttcttttt
     6181 gagcgacgca aagtcggcgg cttcggccgc ctttttgttg gtgacgagta catcgatccg
     6241 atcgaccgga cagtacgaga tccggctgcg ctggccgatc ttgctgtagt cggcgaggat
     6301 caccacgcgg tctgcgttag cgaccatggc gcgcgccact tcggtttcag catgatcgta
     6361 gttggtcgcg ccatgccggt gatcgatacc gaccggcgag agcaaggcca tatccgcccg
     6421 atagcgttgg atgtcgagca cggtagtggc gccggtggtc gccatggcgc gatcgctgat
     6481 cgaaccgccc agcaggatca cttcgttggc ggcttccgtt tggtccacgg cgccgcgcat
     6541 tttcagcgcg acgtcgatcg aattggtgac gatcgtgagg ttcgccagct tggccagctc
     6601 ttcagtaagc gccgccgtgg tggtgcccgc atcgatgaaa agcgtctggc cgctcgcgat
     6661 caaccgcgtg gccgccttgg cgatcgccgt tttggctttg acacgcatgt gggcgcgctc
     6721 ggcgattggc gcttcctcag cgggtttgat cgcgccgcca tgcacgcgcc gcaattcacc
     6781 cagcgcttcc aggtcgagca gatcgcgccg cacggtttcg cgcgacaccc cgagatccgc
     6841 catgatccgt tcggtcgaca cgcgttgaag cgtggacagc aacgcgcgaa ttctctgatg
     6901 acggtcttcc tgccacatgc attttccctt ggatgtgaac gcgcgctcac acggcttgtt
     6961 tttgatcgtg tcatcgtgga catggatgtg tgtccggacg atgtcaagcg tactacttta
     7021 gccgattgtg ttgtattttt ttggcagctt gcaagtttgt gtatttcacc ttagccttcg
     7081 cagatcgaag cacggcaccg ttttcgaaca tcaaatgttc ggaaaggaat ttgaaatgtc
     7141 cgattccgag cattttgcga cgcttttttg gcactttgat tgactcgtgc cgacgccata
     7201 atggatctcg ggatccgcgg cctgaaccat ttgctgcaac gcgcgacgct gcctgaaagc
     7261 cgcacggttc ggcatcctga caataagatt gccgggcgga cgcgcagctg accgtcgcga
     7321 tgcgcgcgcg gcgctggcca tatggccagc tatcgcagcg tcgcccttgg cgcgatacta
     7381 cgcacggggc tcacgcgtaa gcgctgcacg gtgcggtggt gggatggcgg cagtggcagc
     7441 agggaacgtg tcgagagcat atttaacccg ctctaactgg aaggtaacga gacgtgggaa
     7501 acgggattga tcgcacctta tccgcagact ggccttatcc gaaactggtt gcgcatcgtt
     7561 gcggcggcac gctggcgccg gagaacacgc tggccggttt cgacgcgtgc gtgcgctacg
     7621 gctaccgcat ggtcgaattc gacgccaaac tctccgccga caaccagctc tttctgctcc
     7681 acgacgacac gctggaacgc acgaccaacg gccagggcgc cgctgccgaa cacacgtgga
     7741 aacaactggc cgcgctcgat gccggcgcct ggttcgggcc gcagttcgcc ggcacgcacc
     7801 tgccaacact agccaaggcc gccgggcgtt gcgcacagga tggccttgcc gccaacatcg
     7861 aaatcaaacc ctgtccgggc cgtgacgaga tcactgggcg cctcgtcgca agcggcgcac
     7921 tgactttgtg gcaggggcag acgccgccgc tgctttcatc gttttcgttc gaggcgctcg
     7981 cagcggcgcg cgatgcggcg cccttgctgc aacgcggcat gctgttcgaa gaagtgccgg
     8041 ctgactggct gcgcatcgtg cgggaactgg actgcgtgtc attgcatgcg agccaccggt
     8101 atttgaccga gccgctggtc gctgagatca aggcgaccgg gttgcgcgtg ctggcctaca
     8161 cggtgaacga tcctgagcgg gcgcggctgc tcgcacaatg gggcgtcgac atgatttgta
     8221 cggatcgcat cgacagactg gaacacgcga tgttttgagc gttgaggaac gggaacccgg
     8281 cgcacaccac gggttgagcc ccggagaacc cggaccgggc actcgccatg tttt
//
LOCUS       DATASH010000962         4309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2142584,
            whole genome shotgun sequence.
ACCESSION   DATASH010000962 DATASH010000000
VERSION     DATASH010000962.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2142584"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..174
                     /locus_tag="VHV99_25650"
     CDS             <1..174
                     /locus_tag="VHV99_25650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008351691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEX3638804.1"
                     /translation="QPVSLLIFLLVPEQATQQHLEILSEIAQLLSDREARERLHTEED
                     RESLHRLLTQWQP"
     gene            351..1319
                     /gene="hprK"
                     /locus_tag="VHV99_25655"
     CDS             351..1319
                     /gene="hprK"
                     /locus_tag="VHV99_25655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HEX3638805.1"
                     /translation="MDTSSINAQSIFDDNAATLKLSWLTGHEGWERGFSSESVANATS
                     SADLVGHLNLIHPNRIQVLGDAEIDYYKRQTDEDRSRHMAELIALEPPFLVVAGGVAA
                     PPELVLRCTRSSTPLFTTPMSAAAVIDSLRLYMSRILAPRATLHGVFLDILGMGVLLT
                     GDSGLGKSELGLELISRGHGLVADDAVDFVRLGPDFVEGRCPPLLQNLLEVRGLGLLD
                     IKTIFGETAVRRKMKLKLIVQLVRRPDGEFQRLPLESQTVDVLGLPISKVTIQVAAGR
                     NLAVLVEAAVRNTILQLRGIDTLRDFMDRQRLAMQDPDSQFPGKLI"
     gene            1457..2350
                     /gene="rapZ"
                     /locus_tag="VHV99_25660"
     CDS             1457..2350
                     /gene="rapZ"
                     /locus_tag="VHV99_25660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_038458391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEX3638806.1"
                     /translation="MRIILITGISGSGKSVALNSLEDAGYYCVDNLPPRFLPQLANYL
                     ADDGQERLAVAIDARSSASLDEMPAMIRDLSRAHDVRVLFLNASTQSLIQRFSETRRR
                     HPLSGSTAHDADVGLLTSLAEAIERERELVAGLAEFGHQIDTSNLRANVLRAWVKRFI
                     EQEDTGLVLMFESFGFKRGVPLDADFVFDVRTLPNPYYDHELRPLTGLDKPVMDFLDA
                     LPVVHEMIDDIEKFLVKWLPHFRDDNRSYLTVAIGCTGGQHRSVFIAETLAARLATSA
                     NVIVRHRDAPVHVGESSKLVA"
     gene            2390..3022
                     /locus_tag="VHV99_25665"
     CDS             2390..3022
                     /locus_tag="VHV99_25665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX3638807.1"
                     /translation="MSSLTVLADVPLFPLHTVLFPDGLLPLKIFEARYLDMARDCLRE
                     KTPFGVCLLKSGAEVAREEEPSVPEAIGCLAEIEECDVEAFGMLLIRARGTRRFRLLS
                     HRVESSGLLVGMAEPLGEDIPLEGNEQLAKFGACAEVLERIIATIRERDPDSLPFAEP
                     FRLEDPSWVSNRLAEVLPIALRARQKLMELQDAGARIDVVHHYMQQHQLL"
     gene            complement(3051..4166)
                     /gene="mutY"
                     /locus_tag="VHV99_25670"
     CDS             complement(3051..4166)
                     /gene="mutY"
                     /locus_tag="VHV99_25670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEX3638808.1"
                     /translation="MPSRLSPSAPSVASAFPVMSDFSARLIAWQRRHGRHDLPWQNTR
                     DPYRIWLSEIMLQQTQVSTVIPYYAKFLARCPDVASLAAAPADDVMALWAGLGYYSRA
                     RNLHRCAQVVVEQHGGVFPGSAEALAELPGIGRSTAAAIASFAFGARATILDGNVKRV
                     LARVFGVEDFPGEKKVENAMWTLAESLLPSNAADDDVSAYTQGLMDLGATLCVRGKPD
                     CLRCPFATDCVANVTGRQRELPTARPKKAVPTRRTWMLVLRDGNAVMLEKRPPSGIWG
                     GLWSLPEAADEAALAERARAFGGDGAVSPLAPFTHVFTHFKLDIEPRLAELDRGVGAL
                     AALGDADTAWVALSDLDSFGVPAPVRKLLDGLHGSLL"
     gene            complement(4174..>4309)
                     /locus_tag="VHV99_25675"
     CDS             complement(4174..>4309)
                     /locus_tag="VHV99_25675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HEX3638809.1"
                     /translation="ESGYFQLDYFVYDRAGLPCRVCGTPIKQIVQGQRSTYFCPTCQR
                     "
BASE COUNT          810 a         1388 c         1307 g          804 t
ORIGIN      
        1 cagccggtct cgctgctgat cttcctgctc gtgcctgagc aggccaccca gcagcacctc
       61 gaaatccttt cggaaatcgc ccagttgctg tccgatcgcg aggcccgcga gcgcctgcac
      121 acggaagaag accgcgaatc gttgcatcgg ctgctcactc agtggcaacc ttgatgcatc
      181 ggcggttgtc cgcacgccga tcgtttgccc gctcgttcgc ctggttaacc gcccgctcgt
      241 ttgtccggtt atccacgtac gcgcgagtac agcccggcat ggttcgcggc tcgcgttagc
      301 gcggccgcac cctttgccga gcaagcggcc cacactggag tcactgactc atggatacgt
      361 ccagcatcaa cgcccagagc attttcgacg acaacgccgc cacgctgaag ctgagctggc
      421 tgacggggca tgaaggctgg gagcgcggct tttcttcgga atcggtcgcc aatgccacgt
      481 cgagcgccga ccttgtcggc cacttgaacc tgatccaccc gaaccggatc caggtgctcg
      541 gcgatgccga aatcgactac tacaagcgcc aaaccgacga agaccgttcg cgccacatgg
      601 ccgagctcat cgcgttggaa ccgccgtttc tagtggtggc gggcggcgtt gccgcaccgc
      661 cggaactggt gctgcgctgc acgcgctcgt ccaccccgct cttcaccacg ccaatgtccg
      721 ccgccgcggt gatcgacagc ttgcgcctct atatgtcgcg cattctggcg ccgcgcgcca
      781 cgctgcacgg cgtgtttctc gacattctcg gcatgggcgt gctgctcact ggcgattccg
      841 gcctcggcaa gagcgaactc gggctagaac tgatcagtcg cggtcacggc ctcgtcgctg
      901 acgatgcagt ggatttcgtg cgcctcggtc cggatttcgt cgaaggacgc tgcccgccgc
      961 tgctgcaaaa tctgctggaa gtgcgcggtc tcggcctgct cgacatcaag acgatcttcg
     1021 gcgaaaccgc ggtgcgccgc aaaatgaagc tgaaactgat cgtgcaactg gtgcgccgtc
     1081 ccgacggcga attccagcgg ttgccgctgg aaagccaaac cgtcgacgtg ctcggcttgc
     1141 cgatcagcaa ggtcacgatt caggttgcgg ccggccgcaa cctcgcggtg ctggtcgaag
     1201 cagccgtgcg caacacgatc ctgcaactgc gcggcatcga tacgcttcgc gacttcatgg
     1261 accgccagcg cctggccatg caggacccgg acagccagtt tcccggcaaa ttgatctgaa
     1321 tccgcggcat tcggcaacac gccgcggcac gccgcagtac tccacggcaa catgaggccc
     1381 acagtcaacg gcaggctgcg gcgccacgcg cacgtggacc agatgctatg atgaactgta
     1441 cggatttcac gactccatgc gcataatcct gatcaccggt atttccggct ccggcaagtc
     1501 agttgccctg aactcgcttg aagacgcagg ctattactgc gtcgacaatc tgccgccgcg
     1561 ttttctgccg caactcgcca actatctcgc cgacgacggc caggaacgcc tcgcggtcgc
     1621 gatcgacgcg cgctcgagcg cctcgctcga cgaaatgccc gcgatgatcc gcgacctctc
     1681 gcgcgcccat gacgtgcgcg tccttttcct gaatgcaagc acgcagtcgc tgattcaacg
     1741 cttctccgaa acgcgccgcc gtcatccgct atccggttcg accgcgcacg acgcggatgt
     1801 cggcctgttg acgtcgcttg ccgaggcgat cgaacgcgaa cgcgaactcg tggccggtct
     1861 cgctgagttc ggccatcaga tcgacaccag caatctccgc gcaaacgtgc tgcgtgcatg
     1921 ggtcaagcgg ttcatcgagc aggaggatac gggtctcgtg ctgatgttcg agtccttcgg
     1981 cttcaaacgc ggcgtaccgc tcgacgccga ttttgttttc gacgtacgca cgctgccgaa
     2041 cccgtactac gatcatgaat tgcggccgct cacgggcctc gacaaaccag tgatggattt
     2101 tctcgatgcg ctgccggtcg tgcatgaaat gatcgacgac atcgaaaagt ttctcgtcaa
     2161 atggctgccg catttccgcg acgacaaccg tagttatctg acggtcgcga tcggctgcac
     2221 gggcggccaa caccgctcgg tgttcattgc ggagacgctc gccgcgcgtc tcgcaacgtc
     2281 ggcaaatgtc attgtgcggc accgcgatgc gccggttcat gtcggcgaat catcgaagtt
     2341 agtggcttaa ctggccgcat cgcgcggcct caactcgaag cgttgcgcca tgtcttcttt
     2401 aactgtgctt gccgatgtac cgctgtttcc gctacatacg gtgctgtttc cggatgggct
     2461 gctgccgctg aaaatcttcg aagcgcgcta cctcgacatg gcgcgcgact gtctgcgtga
     2521 aaaaacgccg ttcggtgtgt gtctgctgaa aagcggtgcg gaagtagcgc gcgaagaaga
     2581 gccttcggtg ccggaggcaa ttggatgcct tgccgaaatc gaagagtgtg atgttgaagc
     2641 cttcggcatg ctgctaatcc gggcgcgcgg cacgcggcgg tttcgcctgc tgtcgcatcg
     2701 cgtggagagc agcggtttgc tggtcggcat ggcggagccg ctcggagaag acatccccct
     2761 cgagggcaac gagcaattgg ccaaattcgg cgcgtgtgcg gaagtgctgg agcggatcat
     2821 cgcaacgatt cgcgagcgcg atccggacag tttgccgttc gccgagccgt tcaggctgga
     2881 agatccgtcg tgggtgtcga accgtcttgc ggaagtgctg ccgatcgccc ttcgtgcaag
     2941 gcaaaagctg atggaattgc aggacgccgg cgcgcggatc gacgtggttc atcactatat
     3001 gcagcaacat caattgcttt gatttgacgt gctttatgct cggaggtttg ctagagcagc
     3061 gagccgtgca gaccatccag cagtttgcgc accggcgcgg gcacgccgaa cgagtcgaga
     3121 tcgcttagcg cgacccatgc ggtgtccgcg tcgcccaatg ccgccaatgc gcccaccccg
     3181 cgatccagtt cagcgagccg cggttcgata tccagcttga agtgcgtgaa cacgtgcgta
     3241 aacggcgcga gcggcgagac cgctccgtcg ccgccgaagg cgcgcgcacg ctcggcgagc
     3301 gcggcttcgt cggcggcttc gggcaggctc cacaaaccgc cccagatgcc cgacggcgga
     3361 cgcttttcca gcatcacggc gttaccgtcg cgcagcacca gcatccacgt gcggcgcgtc
     3421 ggcaccgctt tcttcggccg tgccgtgggc agttcgcgct gacgtccggt gacgttggcc
     3481 acgcagtcgg tggcgaacgg gcagcgcagg cagtccggct tgccgcgcac gcataacgtt
     3541 gcgccaaggt ccatcaggcc ttgcgtgtag gcgctgacat cgtcgtccgc tgcgttggac
     3601 ggcagcagcg attccgccag cgtccacatc gcattctcga ctttcttttc gcctggaaaa
     3661 tcttcgacgc cgaatacccg cgccaatacg cgcttcacgt tgccatcaag aatcgtcgcg
     3721 cgcgcgccga acgcgaaaga ggcgatcgcc gccgccgtcg agcggccaat gcccggcaat
     3781 tccgcgagcg cttcggccga cccgggaaac acgccgccat gctgctcgac caccacctgc
     3841 gcgcagcggt gcagattgcg ggcgcgcgag taatagccga ggccggccca caacgccatc
     3901 acatcgtccg cgggcgcggc ggcgagcgac gcaacatcag gacaacgcgc aagaaacttt
     3961 gcgtagtacg gaattaccgt cgacacctgg gtctgctgca gcatgatttc cgacagccag
     4021 atgcgatagg gatcgcgcgt gttctgccac ggcagatcat gacgaccatg ccgacgctgc
     4081 cacgcaatca gccgcgcgga aaaatcagac atgaccggga aagcagacgc aacagacggg
     4141 gccgacgggc ttaaacgaga aggcattgaa gatttagcgc tgacaggtgg gacagaaata
     4201 ggtggaacgc tgcccctgca cgatctgttt gatcggcgtt ccacacacgc ggcatggcag
     4261 ccccgcgcga tcatagacga aatagtcgag ctggaagtaa ccgctttcg
//
LOCUS       DATASH010000963        14023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_956720,
            whole genome shotgun sequence.
ACCESSION   DATASH010000963 DATASH010000000
VERSION     DATASH010000963.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 14023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14023
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956720"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..1143
                     /locus_tag="VHV99_25680"
     CDS             202..1143
                     /locus_tag="VHV99_25680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011353408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase A"
                     /protein_id="HEX3638810.1"
                     /translation="MKILFYMPHSDSAAWLHDLTRALPQAELREWQAGDTKPADFAVT
                     WRPPREMLAGRDDLRAIFNLGAGVDAILSLEHEHPGTLPRNAQLIRLEDTGMAAQMAE
                     YVTHAVLRYLRRFDEYQTLQGERRWHVLDAHPRETFTVGVLGLGVLGAHVAQAVAAFG
                     MPVRGYSRSARQIDGVTTFAGAAQFDAFLDGVKVLVNLLPHTPDTGDVLNQRTFSKLA
                     KGAYLINVARGGHLVEQDLLEALASGQLAAATLDVFREEPLPPDHLFWQEPRITITPH
                     VSALTLREESVAQVARKMAALMRGETVSGVVDIERGY"
     gene            1422..2348
                     /locus_tag="VHV99_25685"
     CDS             1422..2348
                     /locus_tag="VHV99_25685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEX3638811.1"
                     /translation="MALPQHVKIVEVGPRDGLQNEKEFVPTAIKIELINRLSAAGFRN
                     VEAASFVSPKWVPQMADGADVMAGIERRAGTIYSVLTPNLRGFEGALAARADEIVIFG
                     AASEAFSQKNINCSIAESIDRFAPVAQAAQEHGLRIRGSVSCALGCPYQGDVPVASVV
                     DVVERFAALGCDEIDIADTIGVGTPKRTREVFEAVTRVFPRERLSGHFHDTYGQALAN
                     IYAALQEGVEIYHASVAGLGGCPYAKGATGNVATEDVLYLMNGLGIETGIDLAQVVAI
                     GDFISTSIARPNVSRAGKALLAKARSEQPNCV"
     gene            2378..2899
                     /locus_tag="VHV99_25690"
     CDS             2378..2899
                     /locus_tag="VHV99_25690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX3638812.1"
                     /translation="MTDHASLDSDDLAALPDSARRVALLLRERGHAGRIVMLPETGKT
                     SAEAAAGLGCSVAQIAKSILFRRREDDAPVLVIASGANRVDEKKVAAQVGEIGRADAK
                     FVREKTGYAIGGVCPIGHATEPVTLIDADLLALESLWAAAGHPHAVFNLTVQELIALT
                     GAPAIDVALRETV"
     gene            2904..3140
                     /locus_tag="VHV99_25695"
     CDS             2904..3140
                     /locus_tag="VHV99_25695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HEX3638813.1"
                     /translation="MTAGIDSEDDEAAVPSPCISVCKMDASTGWCEGCLRTIDEIAGW
                     SLFDDDAKRAVWDAIEERHAQFMAKQTNPAKVQR"
     gene            3137..3646
                     /locus_tag="VHV99_25700"
     CDS             3137..3646
                     /locus_tag="VHV99_25700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX3638814.1"
                     /translation="MNAAPRIVTIGETFHSTLELSADSVKSFATLVNDFNPLHHDEAY
                     AAQSRFGGLIASGTQPTAHFMALLATHFSTYAQPLGLEFDIKLKKAVHANDTLAITWR
                     VRDAYWKPSLNGDLTHLEGSVVNQRGETMLTGTSTILVMAKPEASESANADSDVDTSV
                     NTRTNTNAP"
     gene            3643..4563
                     /locus_tag="VHV99_25705"
     CDS             3643..4563
                     /locus_tag="VHV99_25705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX3638815.1"
                     /translation="MTRLPESIRVFERGWLSSNNVLLVDDTCAALVDTGYATHAPQTV
                     ALVQKTLGARPLDLIVNTHLHSDHCGGNALLQSAWPCRIAIPASEADAVRDWDESRLT
                     FRATGQRCERFSFTETITPGAQLRLGALNWQVLGAPGHDPHSLMLYCAAERILISADA
                     LWENGFGVIFPELEGESGFAEEQAVLEAIAKLDVRLVIPGHGSPFTNVEQALEQAFSR
                     VAWLRADPARNARNALKVLIVFKLLEVRAMSFDTLLQMLDDAAVMRAAAIMLKPRGEW
                     PALIRTIVDELAARNGPLVIDGERIVAREA"
     gene            complement(4969..5868)
                     /locus_tag="VHV99_25710"
     CDS             complement(4969..5868)
                     /locus_tag="VHV99_25710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX3638816.1"
                     /translation="MASNEIRRGAAEMSMAMLMSGTIGWLVVSSRQSPFNVVFFRCIF
                     GGATLALVCAVLGLFRRKLFSWKMLGLALLGGAAIVINWVLLFAAYSRASISMATAVY
                     NTQPFMLVALGALVFRERISASTLAWLVVAFIGLVFVVKVEPAVLAMPGHYLVGVAYA
                     VGAGAMYAVSSIITKHLKGTPPHLIALIQVSLGIVMLAPFVRFDALPTSGVQWLELIV
                     LGIVNTGLMYVLLYGAIQKLPTSMTGALSFIYPVVAIIVDRVAFGQTLAWIQVLGAVL
                     ILLAAAGVNLGWRIVPQKRLSST"
     gene            5983..6453
                     /locus_tag="VHV99_25715"
     CDS             5983..6453
                     /locus_tag="VHV99_25715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX3638817.1"
                     /translation="MTKRLTSPIPAILDETDRALLAALAADARQPVSELARLVGLSAP
                     STAERVRRLEAQGVIERFTVQIDPRALGFTLQAIVRVKPLPGQLHLVEDVIRRIPEFV
                     ECDKVTGDDCFICRLYLHSIDQLDEILSKVTERAETSTAIVKSTPVARRLPPLG"
     gene            complement(6634..7524)
                     /locus_tag="VHV99_25720"
     CDS             complement(6634..7524)
                     /locus_tag="VHV99_25720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3638818.1"
                     /translation="MAFEDFTPFRVAVGDVDIFGVKGGAGPPLLLLHGHPQSHLIWQR
                     CAAPLAEHFTVIATDLRGYGASGKPPTDAGHTPYSKRVMAAEQVAVMRHFGFERFLLC
                     AHDRGARVAHRMALDHADAVERLMLLDIAPTLAMYEATDRAFATLYFHWFFLIQPEPL
                     PETLIGANPAAYVDAVMGSRHAGLAPFAPEALDAYRSALAQPGAVHAMCEDYRASASI
                     DLEHDRADIERGHKIGCPLRVLWGDKGVIEKCFDALAEWRHVARDVSGRSLSCGHYIP
                     EEAPDELVAEMLSFFEAVEE"
     gene            7834..8793
                     /locus_tag="VHV99_25725"
     CDS             7834..8793
                     /locus_tag="VHV99_25725"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015001449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HEX3638819.1"
                     /translation="MALPAVLQKLALPVVASPMFIVSYPELVLAQCKAGIVGSFPALN
                     ARPAELLDEWLTQIQTQLAEHKAANPGAIIGPIAVNQIVHQSNTRLEHDVRVCVEHKV
                     PIFITSLRAPAREIVDAVHSYGGIVLHDVINLRHAQKALEAGVDGLILVASGAGGHAG
                     TTSPFALVGEVRRMFDGPIVLSGSIANGGSILAAQAMGADLAYMGTRFIATREAHAVD
                     SYKQAIVNSTASDIIYTNLFTGVHGNYIRESIVNAGLDPEALPESDKTAMNFGSDKAK
                     AWKDIWGAGQGVGLMDDVPSVGELVQRLTNEYNDAKARLGIAR"
     gene            complement(8869..12330)
                     /locus_tag="VHV99_25730"
     CDS             complement(8869..12330)
                     /locus_tag="VHV99_25730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX3638820.1"
                     /translation="MTDLSTPQRAGGHKLPAGRRLRFVTAAALFDGHDASINIMRRIL
                     QTSGVEVIHLGHNRSVDEVATAALQEDADGVAVSSYQGGHNEYFRYLVDLLRARGGER
                     IKVFGGGGGVIVPEEIAELERYGVEKIYSPHDGQRLGLQGMIDDMIGRCAEVARAAAA
                     VRESPVGEWVADLSACGLPNFANSNEQPDPAAFTFRRVAQLISAYEAGRVRSAEQEIL
                     SVLAEATEVPVLGITGTGGAGKSSLTDELIRRFRLDYGDALTIAVLAIDPSRRKSGGA
                     LLGDRIRMNAIGDWGGGARVYMRSMATRDASSEITSSLEDVLTLCKAAGFDLIIVETS
                     GIGQGDAAIVPFVDEPLYVMTPEFGAPSQLEKIDMLDFASFVAINKFDRKGAPDALRD
                     VAKQVQRNRGDFTKAPDAMPVFGTIASRFNDDGVTALYHHVAEALRSHGLRAGGGRLP
                     PVGDLRFSSGRNAIVPPARVRYLADVAQTVHAYRERANAQARVARERWQLTEARRMLG
                     ETDAGAGAGAGAGTDTDTGAGTGTGTGTGTGTSAAASSANTPQTQLDALIAQRTAALG
                     EHERKLLDAWPQTVAAYSGDEHIVRIRDREIHTALTVTTLSGCEVRKVALPKFVDHGE
                     ILRWLMLDNLPGYFPFTAGVFPFRRENEDPTRMFAGEGDPLRTNRRFKLLSDGMPAKR
                     LSTAFDSVTLYGEEPDERPDIYGKVGNSGVSVATLDDMKTLYDGFDLCAPQTSVSMTI
                     NGPAPTILAMFFNVAIDQQIARLAQQQGRPLTEDELAAARRTALENVRGTVQADILKE
                     DQGQNTCIFSTEFSLKVMGDIQAYFVDHGVRNFYSVSISGYHIAEAGANPISQLAYTL
                     ANGFTYVEAYLARGMSIDDFAPNLSFFFSNGMDPEYTVLGRVARRIWAIAMRERYGAN
                     ERSQKLKYHVQTSGRSLHAQEIDFNDIRTTLQALIAIYDNCNSLHTNAFDEAITTPTE
                     DSVRRAVAIQLIINREWGLAKNQNPNQGSFVIEELTDLVEEAVLTEFDRLTERGGVLG
                     AMESGYQRGRIQDESMLYEHRKHDGSYPIVGVNTFLSAHPHEAPQPIALARSTDEEKQ
                     SQLKRLRDFQARHRDDAPAALERLKRAVIDDDNVFAVLMDVVRVCSLGQITHALFEVG
                     GQYRRNM"
     gene            complement(12396..13334)
                     /locus_tag="VHV99_25735"
     CDS             complement(12396..13334)
                     /locus_tag="VHV99_25735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3638821.1"
                     /translation="MDTLVSMKVFRHVVEVGSFVGAAERMEMSAAMASKHVMHLEQQL
                     GARLLNRTTRRVAPTEAGREYYERLSQALTELEEAGQVVGAASVVPQGRLRVSSLSAF
                     GLSHVMAAVADYASQYPQVTVDMTLSDRVVELIDEGYDVAIRASPSGLKSSSLIARQI
                     ATAHLVLCASPAYLRRHGTPKTIADLARHNYLQYAGVSALEIASATGDGSPRVRLSGN
                     LIVNHLEAQRVIVLQGAGIAMLGTEVIGDDLAAGRLVPLLVDDVPPRELPIHVVYASR
                     RHLSAKVRSFVDFLAERFANEALWPSLEQIKALAVR"
     gene            complement(13598..>14023)
                     /locus_tag="VHV99_25740"
     CDS             complement(13598..>14023)
                     /locus_tag="VHV99_25740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX3638822.1"
                     /translation="ATAVYQYVNFNNVPGDLGSFDTSGVPGLKSQSVAQAGVTYDLKF
                     VKFFGQYMYTYNQQTISSWHVNTGQGGVTVPVGPGTVMASYAYSRDGGGLDQTRQTAS
                     LGYDYPLSKRTDVYAAYMYDKISSQSSGNTYGVGMRAKF"
BASE COUNT         2665 a         4425 c         4470 g         2463 t
ORIGIN      
        1 ggggcaagcg agctttgccg cccaaaccgc acgtccgtgc gcaaacgcgc gcgagtgctc
       61 tcgaaaaagt agaatgaagc ctatccataa aacgaggtgg tccatgccgc aagcgtcgtc
      121 tgcagcgccg cgggcgttgc agcccatcta cccagccttt cccgccatca gactcgataa
      181 cgcagacgcc ttccagaccg tatgaaaatc ctcttctaca tgccgcattc cgactccgcc
      241 gcgtggcttc acgacctgac ccgcgcgctg ccgcaagcgg aactgcgtga atggcaggca
      301 ggcgacacca aacccgccga tttcgcggtg acatggcgtc cgccgcgtga gatgctggcc
      361 ggccgcgacg atctgcgcgc catcttcaat ctcggcgcgg gcgtcgatgc gatcctctcg
      421 ctcgaacacg agcaccccgg cacgttgccg cgcaacgcgc agttgatcag gctcgaagac
      481 acgggtatgg ccgcgcagat ggcggagtat gtcacccatg ccgtgctgcg ctacctgcgc
      541 cgcttcgacg agtatcagac gctgcaaggc gagcgccgct ggcacgtgct cgatgcccat
      601 ccgcgcgaga ccttcaccgt cggcgtgctc ggcctgggtg tgctcggcgc gcatgtcgcg
      661 caggcagtcg cggccttcgg catgccggtg cgcggatata gccgcagtgc gcggcaaatc
      721 gacggcgtga cgaccttcgc aggcgccgcg cagttcgatg cgtttctcga cggcgtgaaa
      781 gtgctggtta acctgttgcc gcacacgccg gatacgggcg acgtgctgaa ccagcgcact
      841 ttctcgaagc tcgcgaaggg tgcgtatctg atcaatgtcg cgcgtggcgg gcatctggtc
      901 gagcaggatc tgctcgaagc gctcgccagc ggccagctcg ccgccgcgac gctggacgtg
      961 ttccgtgagg aaccgttgcc gcccgatcat ctgttctggc aggagccacg cattacgatc
     1021 acaccgcatg tgtccgcact cacgttgcgc gaagagagtg tcgcgcaagt cgcccggaaa
     1081 atggcggcgc tgatgcgcgg cgaaacggtg agcggcgtgg tcgatattga gcgcgggtac
     1141 tgagcgtggg tagcaggtat ggatattcga cgtggacagc gagccgatac tggatacgga
     1201 tatcgaacac agttttgaac agtatcgaat caaactgaag gagcgccgcc acgcgcgtgg
     1261 ctgattgcat gggaccggag taatgcgcta aagcgcaact ccggtcggcc gctttgcatg
     1321 ggaccggagt aatgcgctaa agcgcaactc cggtcgaccg ctttgcatgg gaccggagta
     1381 atgcgctaaa gcgcaactcc ggtcgacaca ggagacacat catggccttg ccccagcacg
     1441 tcaaaatcgt cgaagtcggt ccgcgtgacg gactgcagaa cgagaaagaa ttcgtcccaa
     1501 ccgcgatcaa gatcgagttg atcaaccgct tgtcggcggc aggttttcgc aacgtggaag
     1561 cggcgtcgtt cgtatcgccg aaatgggtgc cgcagatggc cgacggcgcc gatgtcatgg
     1621 ccggcatcga acgccgtgca ggcacgatct actcggtgct gacgccgaac ctgcgaggat
     1681 tcgaaggcgc actcgccgcg cgcgcggacg agatcgtgat cttcggcgcg gccagcgaag
     1741 cgttctcgca gaagaacatc aattgcagca tcgcggaaag catcgatcgg ttcgcgccgg
     1801 tcgcgcaggc ggcgcaggag catggcctgc ggattcgcgg cagcgtgtcg tgtgcgctgg
     1861 gttgtccgta tcagggcgac gtgccggtgg cgtcggtggt cgatgtggtc gagcgctttg
     1921 ctgcgctcgg ctgcgatgag atcgatatcg cggatacgat cggcgtcggc acgccgaagc
     1981 gcacgcgcga agtgttcgag gccgtcacca gggtgtttcc gcgtgaacgt ctgtcgggcc
     2041 actttcacga cacctacggt caggcgctcg cgaacatcta cgccgcgttg caggaaggtg
     2101 tcgagatcta tcacgcctca gtggcggggc tcggcgggtg cccgtatgcg aagggcgcaa
     2161 ccggcaacgt cgcgaccgaa gacgtcctgt atctgatgaa cggcctcggt atcgagaccg
     2221 gcatcgacct tgcacaagtc gtcgcgatcg gcgatttcat ttcgacgtcg attgcccggc
     2281 cgaacgtatc gcgcgccggc aaggcgctgc tcgccaaagc gcgcagcgaa cagcccaact
     2341 gcgtttgact cttgccatcc cttcaggaca tagaacgatg acggaccacg cttctctcga
     2401 ttccgacgat ctcgccgcgt tgcctgactc cgcccgccgc gtcgcgctgc tgttgcgcga
     2461 acgcggccac gcgggacgga tcgtgatgct gccggagacc ggcaagacct ccgccgaagc
     2521 cgctgccggc cttggctgct cggttgcgca aatcgccaag tcgatcctgt ttcgccgccg
     2581 tgaagacgat gcgccggtcc tcgtgatcgc gagcggcgcg aaccgtgtcg acgaaaagaa
     2641 agtcgcggcg caagtcggcg agatcggccg tgcggatgcg aagttcgtcc gcgagaaaac
     2701 cggttacgcg atcggtggcg tgtgcccgat cggccatgcc acggaaccgg tcacgctgat
     2761 cgacgccgat ctgctggcgc tcgagagcct gtgggccgcc gcgggtcatc cgcatgcggt
     2821 gttcaatctg accgtgcagg agctgatcgc attgacgggt gcgccggcca tcgacgtggc
     2881 attgcgcgag acggtatgaa gccatgacag cgggcatcga tagcgaagac gacgaggcgg
     2941 ccgtgccctc accctgcatc agcgtgtgca agatggatgc gtccaccggt tggtgcgaag
     3001 gctgtttgcg cacgatcgac gagatcgcgg gctggtcgtt gttcgacgac gacgcgaagc
     3061 gcgcagtctg ggacgcaatc gaggagcgtc acgcgcagtt catggcaaag cagacaaacc
     3121 cggcgaaggt gcaacgatga acgccgcacc gcgcatcgtg acgatcggcg agacgttcca
     3181 ctcgacgctc gaactatcgg cggactcggt gaaatctttc gccacgctcg tcaacgactt
     3241 caacccgctg catcacgacg aggcgtacgc cgcgcaaagc cgctttggcg gtttgatcgc
     3301 atccggcacg caacccaccg cgcatttcat ggcgttgctg gccacgcact tctcgaccta
     3361 cgcacagccg ctcggcctcg aattcgatat caagctgaag aaggcggtcc atgcgaacga
     3421 cacgctggcg atcacctggc gcgtgcgcga tgcttactgg aagccgagtc tgaacggcga
     3481 tctgacgcat ctcgaaggga gcgtggtgaa ccagcgcggc gaaaccatgt tgactggcac
     3541 gtcgacgatc ctggtgatgg cgaaacccga ggcatcagag agtgcgaacg cagacagcga
     3601 cgtggacaca agcgtgaaca cacgcacgaa cacaaacgca ccatgaccag actgccggaa
     3661 tcgatccggg tcttcgaacg cggctggctt tcgtcgaaca acgtgctgct ggtcgacgac
     3721 acctgtgccg cgctcgtgga taccggttac gcaacgcacg cgccgcaaac cgtcgcgctg
     3781 gtgcaaaaaa cgctcggcgc gcgaccgctc gatctgatcg tcaacacgca tctgcattcg
     3841 gatcactgcg gcggcaacgc gctattgcag tcggcgtggc cgtgccgtat cgcgattccc
     3901 gcatccgaag cggacgcagt acgcgattgg gacgaaagcc gtctgacgtt tcgcgccacc
     3961 ggccagcgct gcgagcgctt cagcttcacc gaaacgatca cgcccggcgc gcaactgcgg
     4021 ctcggcgcgc tgaattggca agtgcttggc gcgcctggcc acgatccgca ttcattgatg
     4081 ctgtattgcg cggctgaacg catcctgatc agcgccgacg cgctgtggga aaacggcttt
     4141 ggcgtgattt tccccgagct ggaaggtgaa agcggtttcg ccgaagaaca ggcggtgctc
     4201 gaagccattg cgaagctcga tgtgcggctc gtgattcccg gccatgggtc gcccttcacc
     4261 aatgtcgaac aggcgcttga acaggcgttt tcgcgcgtcg cgtggctgcg cgccgatccg
     4321 gcgcgcaatg ccaggaacgc actgaaggtg ctgatcgtgt tcaagctgct cgaagtccgc
     4381 gcgatgagct tcgacacgtt gctgcaaatg ctcgacgacg ccgccgtgat gcgcgctgcg
     4441 gcaatcatgc ttaagccgcg tggtgaatgg cccgcgctga tccgcacgat cgtcgacgag
     4501 ttggcggcca ggaacggccc actggtgatc gatggcgagc gcatcgtcgc gcgcgaggcg
     4561 tgaaagcgcg ggtgaggtga gcgcgagaag cggggatgca ggtaagcagg gatgcgggta
     4621 agtggggcgc gcgggcattg gcacgcgtat aggccgcata tgacacgtaa aacaccaaaa
     4681 ccgtcgccac aaaaagaaag ccgctcgacc atcaaggcga tcggcggaaa ttctgtcgga
     4741 cgtgatcagc gtcgaggtga atgatacgcg ccccggtttt ttgaccgcat cagtgatttc
     4801 cctagaggga cttgacgcgg ccttttttaa acgcatatac agcgaacgat acgaagcctc
     4861 ggagtcgcga gtggaacgcc attgatccgc ctatatgaac aatgcgcaca tcatccagtt
     4921 gctccgtaat ccgtaatatt ttccgctacc gcatttgcaa cgcgctcctc aggtcgagga
     4981 cagacgtttc tgcggcacga tccgccagcc gagattcacg cccgcggccg cgagcaggat
     5041 cagcaccgcg ccaagcacct gaatccacgc gagtgtctgc ccgaacgcaa cccgatcgac
     5101 gatgatcgcc accacgggat agatgaacga caacgcaccg gtcatcgagg tcggcagctt
     5161 ctggatcgcg ccgtaaagca gcacatacat gagccccgtg ttgacgatac cgagcacgat
     5221 caattcgagc cactgcacgc cgctggtcgg cagcgcatcg aaacgcacga acggcgccag
     5281 catcacgatg ccgagcgaca cctggatcag cgcgatcaga tgcggcggcg tgcccttcaa
     5341 gtgcttcgtg atgatcgacg agactgcata catggccccc gcgcccacgg catacgcaac
     5401 cccgaccaga tagtgacccg gcatcgccag caccgccggc tcgaccttca ccacgaacac
     5461 gaggccaatg aacgcgacca ccagccaggc gagcgtcgac gcactgatgc gctcgcgaaa
     5521 caccagcgcg ccaagcgcga ccagcatgaa aggctgcgtg ttgtagacgg cggttgccat
     5581 cgagatcgaa gcacgcgaat aggccgcgaa cagcaacacc cagttgatga cgatcgccgc
     5641 gccgccgagc agcgcgaggc cgagcatctt ccacgaaaag agcttgcgcc tgaacaggcc
     5701 gagcacggcg cacacgagcg ccagcgtcgc gccgccgaag atgcagcgaa agaacactac
     5761 attgaacggg ctctgtcgcg acgacaccac cagccagccg atggtgccgg acatcagcat
     5821 ggccatgctc atttcggccg cgccgcggcg aatttcgttt gacgccatga ttcgatcctc
     5881 gaattgattc ctgtatgagc agcagtgtaa ttaatccggg cgcccgaata catggctaaa
     5941 cttaagtcgt ctcccgagac cacctaataa tcgaaggcca tcatgaccaa acgccttacg
     6001 tcgcccatac ccgcgatact cgatgaaacc gaccgcgcgc tgctcgccgc actggcggcc
     6061 gacgcgcgcc aaccggtcag cgaactggcg cgtctggttg gcctctcggc cccgagtacg
     6121 gccgaacgcg tccgccggct cgaagcacaa ggcgtgatcg agcggttcac cgtacagatc
     6181 gatccgcgcg cgctcggctt cacgttacag gcgatcgtgc gcgtgaagcc cttgcccggg
     6241 cagctgcatc tggtggaaga cgtgatccgg cggattccgg agtttgtcga atgcgacaag
     6301 gtgacgggcg acgattgctt catctgtcgc ctgtatctgc attctatcga tcagctcgac
     6361 gagattctgt cgaaggtcac cgagcgcgcg gagaccagta ccgcgatcgt taagtcgacg
     6421 ccggttgcgc gccgtttgcc gccgcttggc tgagcgaaaa cgcggggggc gcgtcgcccc
     6481 gcgcttgagc gataccgtgg ccgagtcgtg cggtagacga agtcgtggcc acctctcggg
     6541 tggacggacc ggcggcccca acgcgggtga acgaacggcg gccgcgtcgc gcatgaacga
     6601 cccggcgccc gcattgcata taaacgattt gacttactct tcgaccgctt cgaagaacga
     6661 cagcatttcg gcaaccagtt cgtccggcgc ttcttcagga atgtaatgcc cgcacgacaa
     6721 cgaacgcccg ctcacgtccc gcgccacgtg gcgccattcc gccagcgcgt cgaagcactt
     6781 ctcgatcacc cccttgtcgc cccacagcac gcgcagcgga cagccgatct tgtggccacg
     6841 ctcgatatcg gcccggtcgt gttcgaggtc gatgctcgcc gacgcccggt agtcctcgca
     6901 catcgcatgc acggcgccag gttgcgccag cgcgctgcga tacgcgtcaa gcgcttcggg
     6961 tgcgaacggc gccaggcccg cgtgacggct gcccatcacc gcgtcgacat aggcggccgg
     7021 gttcgcgccg atcagcgtct ccgggagcgg ctccggctgg atcaggaaga accagtggaa
     7081 gtaaagcgtc gcgaaggcgc gatcggtggc ttcatacatc gcgagcgtgg gcgcgatgtc
     7141 gagcagcatc aaacgctcga cggcgtcggc atgatcgagc gccatccggt gcgcgacccg
     7201 ggcgccgcga tcatgcgcgc acagcaggaa tcgttcgaag ccgaagtggc gcatcacggc
     7261 gacctgctct gccgccatca cgcgtttgga atacggcgtg tgcccggcgt cggtgggcgg
     7321 tttgcccgag gcgccgtagc cgcgcaagtc ggtggcgatg acggtgaaat gttcggccag
     7381 tggcgccgcg caccgctgcc agatcagatg cgactgcggg tgaccgtgca gcaacagaag
     7441 gggcggccct gccccgccct tcactccgaa aatatcgacg tcgcccacgg cgacgcgaaa
     7501 gggcgtgaaa tcctcgaagg ccatagcgag tctcttgtcg tttgggagtg aaagcattgt
     7561 aggagcgcaa cgtgccggcg cggggtgggt taggccacgc aagcatagaa actgaagact
     7621 ccctccaacc gttcgaaaca gacacgactc gaccgcttcg cctataatcg aacgatcgtt
     7681 cttaaatttt ggggcgcggt ggcaatgcgt agcggggttg tgacggcagc atgacgctcg
     7741 tcaacctcga cgcaccacag gccatgtgcc gctaaactca tattcgccgg gcgctctgcg
     7801 ccgcaccggt ccgttcatca ggagactcgc accatggcat tgcccgccgt cctgcaaaaa
     7861 ctcgcgctgc ccgtcgtcgc ttcgccgatg ttcattgtca gctatcccga actcgtgtta
     7921 gctcaatgta aggcgggcat tgtcggctcg ttccccgcgc tgaatgctcg cccggccgaa
     7981 ctgctcgacg aatggctcac gcagatccaa acacagctcg ccgaacacaa ggccgccaat
     8041 cccggcgcga tcatcgggcc gattgccgtc aatcagatcg tccatcagtc gaatacgcgg
     8101 ctcgagcacg acgtgcgggt gtgcgtcgaa cacaaggtgc cgattttcat caccagcctg
     8161 cgcgcgccgg cccgggagat cgtcgacgcg gtgcatagct acggcggcat cgtgctgcac
     8221 gacgtgatca atctgcggca tgcgcagaag gcgctggaag cgggtgtcga tgggctgatc
     8281 ctggtggcgt cgggtgccgg cggccatgcg ggcacgacct cgccgttcgc gctggtcggc
     8341 gaggtgcggc gcatgttcga cggcccgatc gtgctgtccg gctcgatcgc caacggcggc
     8401 tcgattctcg ccgcgcaagc catgggggcg gacctcgcgt acatgggcac gcgcttcatc
     8461 gcgaccaggg aagcgcacgc ggtcgacagc tataaacagg cgatcgtcaa ttccaccgcg
     8521 tcggacatca tctacacgaa cctgttcacc ggcgtgcacg gcaactacat ccgcgaaagt
     8581 atcgtgaacg cggggctcga tccggaggcc ctgccggaat cggacaagac cgcgatgaac
     8641 ttcggcagcg acaaggcaaa ggcgtggaaa gacatctggg gcgcaggcca gggggtcggc
     8701 ctgatggacg atgtgccgag cgtgggcgag ctggttcagc gtttgacgaa cgaatacaac
     8761 gacgcgaaag cgcggctcgg catcgcacgg taagtcgttg agtatgatcg agtgacggta
     8821 ttgcagcggc gccgcctgag gcgaagccgc tgcacggcaa gcaacgattc acatattgcg
     8881 gcggtactgc ccgccaactt caaacaacgc gtgcgtgatt tgccccagcg agcagacgcg
     8941 caccacgtcc atcagcaccg cgaacacgtt gtcgtcgtcg atcactgcgc gcttcaaacg
     9001 ctcaagcgcc gcgggtgcgt cgtcgcgatg ccgcgcctga aaatcgcgta gacgcttcaa
     9061 ctggctctgc ttctcttcat cggtggagcg ggccagcgca atcggctgcg gcgcttcatg
     9121 cggatgcgcg ctcaagaacg tgttcacacc aacaatcgga tacgagccgt cgtgcttgcg
     9181 atgctcatac agcatcgact catcctgaat gcgcccgcgc tgatagccgc tttccatcgc
     9241 gccgagcacg ccgccgcgct cggtgagtcg atcgaattcc gtcagcacgg cttcttccac
     9301 cagatcggtc agctcctcga tcacgaagct gccctgattc ggattctgat tcttcgcgag
     9361 gccccattca cggttgatga tcaactggat cgccaccgca cggcgcaccg aatcctcggt
     9421 gggcgtggtg atcgcttcgt cgaacgcatt ggtgtgcagc gaattgcagt tgtcgtagat
     9481 cgcgatcagc gcttgcagtg tggtgcggat atcgttgaaa tcgatctcct gcgcgtgcag
     9541 gctgcggccc gaggtttgca cgtggtattt gagcttctgg ctgcgttcgt tggcgccata
     9601 acgctcgcgc attgcaatcg cccagatgcg tcgtgcgaca cgaccgagca cggtgtattc
     9661 cgggtccatg ccgttcgaaa agaagaacga cagattcggc gcgaaatcgt cgatcgacat
     9721 gccgcgcgcg agataggctt cgacatacgt gaagccgttc gcgagcgtat aggccagctg
     9781 cgagatcggg ttcgcgccgg cctcggcaat gtgatagccg gagatcgaca ccgaatagaa
     9841 attgcgcacg ccgtgatcga caaagtacgc ctgaatatcg cccatgactt tcaggctgaa
     9901 ctcggtcgag aagatgcagg tgttctggcc ttgatcttcc ttcaggatgt cggcttgcac
     9961 cgtgccgcgc acgttttcca gcgctgtgcg tcgcgcggcg gcgagttcgt cttctgtgag
    10021 cggacggcct tgctgctgcg ccaggcgggc gatctgctga tcgatcgcga cgttgaaaaa
    10081 catcgcgaga atcgtcggcg ccgggccgtt gatcgtcatc gataccgacg tctgcggcgc
    10141 gcacagatcg aagccgtcgt agagcgtttt catgtcgtcg agcgttgcaa ccgagacgcc
    10201 ggaattaccc accttgccgt agatgtcggg ccgttcgtcc ggctcttcgc cgtagagggt
    10261 gaccgaatcg aaggccgtcg aaagacgttt ggccggcatg ccgtcggaga gcaatttgaa
    10321 acgacggttg gtgcgcaacg gatcgccttc gccggcgaac atgcgcgtcg ggtcttcgtt
    10381 ttcgcggcgg aatgggaaca cgccggcggt gaacggaaaa tagccgggga gattgtcgag
    10441 catcagccag cgcaggatct cgccgtggtc cacgaatttc ggcagcgcga ctttgcggac
    10501 ctcgcaaccg gatagtgtcg tgacggtgag cgctgtgtgg atctcacggt cgcgaatgcg
    10561 aacgatgtgt tcgtcgccgg aataggcggc gacggtttgc ggccaggcgt cgagcagctt
    10621 gcgttcatgt tcgccgaggg cggcggtgcg ttgggcgatg agggcgtcga gttgggtttg
    10681 gggcgtgttc gcggagcttg ccgcagcgct tgtgcctgtg cctgtgcctg tgcctgtgcc
    10741 tgtgcctgcg cctgtgtctg tgtctgtgcc tgcgcctgcg cctgcgcctg cgcctgcatc
    10801 ggtttcgccg agcatccggc gcgcttcggt gagttgccag cgctcacgcg ccacgcgcgc
    10861 ttgcgcgttg gcacgctcgc ggtacgcgtg gacggtttgc gcgacatccg ccagatagcg
    10921 cacgcgggcc ggcggcacga tggcgttgcg gccactcgaa aagcgcaagt cgccgaccgg
    10981 cggcagacgt ccgccgccgg cgcgcaagcc atgcgacctc aacgcctcgg caacgtggtg
    11041 atagagcgcc gtcacgccgt cgtcgttaaa gcgcgaagcg attgtgccga acacgggcat
    11101 cgcgtcgggc gcctttgtga agtcgccgcg attgcgctgg acctgcttcg ccacgtcgcg
    11161 caatgcatcc ggcgcaccct tgcgatcgaa cttgttgatc gcgacgaagc tggcaaagtc
    11221 gagcatgtcg atcttttcca actggctcgg cgcgccgaac tccggcgtca tcacatagag
    11281 cggctcgtcg acgaacggca caatcgccgc gtcgccctgc ccgatcccgg acgtctcgac
    11341 gatgatcaga tcgaagcccg cggccttgca cagcgtgagc acgtcttcca atgaactcgt
    11401 aatttcgctc gatgcgtcgc gcgtcgccat cgagcgcatg tagacgcgcg cgccaccgcc
    11461 ccaatcgccg attgcgttca tgcggatgcg gtcgccgagc agcgcgccgc cggatttgcg
    11521 gcgcgacggg tcgatcgcca gcacggcgat ggtgagggcg tcaccgtagt cgagccgaaa
    11581 gcggcggatc agctcgtccg tgagcgagga tttgccggcg ccgccggtgc cggtgatgcc
    11641 gagcaccggc acctccgtcg cttcagcgag gacggagagt atttcctgct cggccgatct
    11701 gacgcggccc gcttcgtacg cgctgatcaa ttgcgcgacg cggcggaagg tgaaggcggc
    11761 ggggtctggc tgttcgttgg agttggcgaa gttcggcaac ccgcacgccg acagatccgc
    11821 cacccactcg ccaaccggac tctcccgtac ggcggcagcg gctcgcgcca cttcagcgca
    11881 gcgcccgatc atgtcgtcga tcatgccttg caggccgagc cgctggccgt catgcggcga
    11941 atagattttc tcgacaccat aacgttcgag ttcggcaatt tcttcaggga cgatcacgcc
    12001 gccaccgccg ccgaacacct tgatacgctc gccgccgcgc gcacgcagca gatcgacgag
    12061 atagcggaag tattcgttgt ggccgccctg gtagctcgac acggcaacac catcggcatc
    12121 ctcttgcagg gcagcggtgg cgacttcgtc gaccgagcgg ttgtggccga ggtggatcac
    12181 ctccacgccg ctcgtttgca gaatgcgccg catgatgttg atcgacgcgt cgtggccgtc
    12241 gaacaaggcg gcggcggtga caaagcgcag gcgacggccc gcgggcagct tgtggccgcc
    12301 agcgcgctgc ggcgtggaca gatcagtcat gtctcccccc ggatcgttac gcgtatgggt
    12361 gctggcaacc agtatagcca aatggatgtg agcgcctaac gcaccgccag cgccttgatc
    12421 tgctcgagcg aaggccaaag cgcttcgttc gcaaaccgct cggcaagaaa gtcgacaaac
    12481 gagcgcacct tggccgacag atgccggcgg ctcgcgtaga ccacatggat cggcaactcg
    12541 cgcggcggca cgtcatccac caacagcggc acgaggcgcc ccgcggcgag atcgtcgccg
    12601 atcacctcgg tgccgagcat ggcgatgccc gcgccctgca gcacgatgac gcgctgcgcc
    12661 tccaggtggt tgacgatcag attgccggac agccgcacgc gcggcgagcc atcgccggtc
    12721 gcggaggcga tttcgagcgc ggacacgccc gcgtattgca gatagttgtg gcgcgccaga
    12781 tcggcgatgg tcttcggtgt accgtgccgg cgcagatagg cgggcgacgc gcacaacacc
    12841 agatgggcgg tcgcgatctg ccgcgcgatc agcgaagacg acttcagccc gctcggcgaa
    12901 gcccggatcg cgacgtcgta gccttcgtcg atcagttcga ccacgcggtc cgacagggtc
    12961 atgtcgacgg tgacctgcgg gtactgcgag gcgtagtcgg ccaccgcggc catcacgtgg
    13021 ctcaaaccga atgccgacag cgacgatacc cgcaagcgcc cttgcggcac gacgctcgcc
    13081 gcgccgacca cctgcccggc ttcttcgagt tcggtgagcg cctggctcaa gcgctcgtag
    13141 tattcgcggc ccgcctcggt cggcgcgaca cgccgcgtgg tgcggttcag caggcgcgcg
    13201 ccgagctgct gttcgaggtg catcacgtgc ttgctcgcca tcgccgccga catctccatc
    13261 cgttcggccg cgccgacaaa gctgccgact tctaccacat ggcgaaacac tttcatgctg
    13321 acgagggtat ccatctcaat cgctcgctgg aggaatcaat caacgccatt ttgccgggtt
    13381 aatcaaaaat tggtcagcaa atcggggcgc tgcgggcgcc agaagcgaca aagcccacct
    13441 tccggagaag gcgggctttg aggtgtcgcc tgattcggca gggcgggctt ggcgtatcgc
    13501 ctgattcagc agggcggcct tggagcatcg cctgattcgg cagggcggct ccagccgctg
    13561 cccgcaagca ggcgccgctc gcctcgcccc ggcacgccta gaacttcgcg cgcatgccga
    13621 cgccgtacgt gttgccgctc gactggctgc tgatcttgtc gtacatatac gccgcgtaga
    13681 catccgtgcg cttggagagc ggatagtcgt agccgagcga cgccgtctga cgggtctggt
    13741 cgaggccgcc accgtcgcgc gaataggcgt acgacgccat caccgtgccc gggccaaccg
    13801 gtaccgtgac gccgccctgc cccgtattca cgtgccagct cgaaatggtc tgctggttat
    13861 acgtgtacat gtactggccg aagaacttca cgaacttcag gtcgtacgtg acgccggctt
    13921 gcgccacgct ctggctcttc aggcccggaa cgcccgacgt atcgaagctg ccgaggtcgc
    13981 ccggcacatt gttgaagttc acgtactgat acaccgcggt cgc
//
LOCUS       DATASH010000964         2223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5706059,
            whole genome shotgun sequence.
ACCESSION   DATASH010000964 DATASH010000000
VERSION     DATASH010000964.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5706059"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="VHV99_25745"
     CDS             complement(<1..809)
                     /locus_tag="VHV99_25745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX3638823.1"
                     /translation="MNDRFVTFKRQPESPQAVGRGSLFGHYSTPSTLPAIRDLRPAAP
                     SVALAGPMRGRSVHQNNDAFFSGKKLAFFLEKTTFSKTKQFFARHRRRLMPATRTMIA
                     RRAGVSVATVDRVLRDDQAVRPETAERVHLALANLRDERASRGRPAKVTSFRVAFVLP
                     QVNSRFLDHVERDIALSASFFREHRITPSFYRCDFSSQRETAAFAAEAIDYDALVLAP
                     LDYPWVERFIEEMSDAHVPVVTVFSDLPASRRAAYVGVDNRIAGRTEGLLMG"
     gene            1349..>2223
                     /gene="oxlT"
                     /locus_tag="VHV99_25750"
     CDS             1349..>2223
                     /gene="oxlT"
                     /locus_tag="VHV99_25750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate/formate MFS antiporter"
                     /protein_id="HEX3638824.1"
                     /translation="MSADLQGGASSWRASPWVQLVFGVICMAMIANMQYGWTLFVNPI
                     NEKYQWGRTAIQVAFTIFVVTETWLVPIEGYLVDKYGPRPVVVVGGLLCAVAWALNSV
                     ASSLPLLYIAAAIGGVGAGAVYGTCVGNALKWFPNRRGLAAGITAAGFGAGSAATVVP
                     IASMIKSSGYEATFLWFGLGQGVIVFILGLALLAPPATLLASAKSAVQRVVYNATPRE
                     VIGSPVFWVMYLMFVMMAAGGLMATAQLGPIAKDFGLHDSPVSLLGLTLPALTFALTI
                     DRVLNGLTRPFFGWIS"
BASE COUNT          445 a          624 c          673 g          481 t
ORIGIN      
        1 cccatcagta gtccttccgt gcgtccggca atcctgttgt cgacacccac gtacgcggcg
       61 cgccggctcg cgggtaaatc ggagaacact gtgacgactg gcacgtgggc gtcgctcatc
      121 tcttcgatga aacgctcaac ccatggatag tcgagaggtg cgagaaccag cgcgtcgtag
      181 tcgatcgctt cagcggcgaa tgccgcggtc tcgcgttgac tggaaaaatc gcagcggtaa
      241 aacgaagggg ttatgcggtg ctcgcgaaag aagctggccg acagcgcaat atcgcgctcg
      301 acgtgatcga ggaagcggga gttcacttgc gggagcacga acgcgactcg gaacgaagtg
      361 acctttgcgg gccggccgcg gctcgcccgc tcatcacgca gattcgcgag tgcaagatgc
      421 acgcgttcag cagtttccgg acgcacagcc tgatcgtcgc gcaggacacg atctaccgtg
      481 gcgacgctga cgccagctcg acgcgcaatc atcgtacgag tagccggcat gagtctcctc
      541 cggtgccgag caaagaattg ttttgtttta ctgaaggtag ttttttctaa aaaaaatgca
      601 agctttttcc ccgagaaaaa agcgtcattg ttttgatgaa cagagcggcc tctcataggg
      661 cccgcaagcg caacggacgg tgcagcggga cgaagatcgc gaatcgcggg gagagtcgat
      721 ggcgtcgaat agtgcccgaa gagagaaccg cggccaactg cctgcggcga ctcaggctgt
      781 cgcttgaacg ttacaaaacg gtcattcaaa ccgcgcctga catggtgcgt taaaggaccc
      841 ctgcccgtat gcgccgtcgt gcaatcacgg gaaagcataa agggcaggcg ctggacacat
      901 cctcacgaat ccgcggttcg atgtcgaacc ggaacttgct tggaacttga accgttactc
      961 cgtgccgctc gcgcaacttc gcgcactctt gctcgataga tcgacgtcga cgagcatcga
     1021 aatttctttc aaataaaaaa ctaacattac tttttacttt catctataaa ggcaaagcat
     1081 gttgcggaca gccttgtccc gttccatgtc ctcggcccgc cggtgttgtc ggcgggctct
     1141 ttttttgcaa cgaaaataat gttggttctt acatacgatt agctttcatt tcaagaatta
     1201 cgtggtgata accctggcca ttctttcact tgacttactt gatataccac atactgtatg
     1261 aggtagatca ccggctatcg cgccggtgac gggcggagaa gccactttgg ccccgcaaac
     1321 atcaggcagt caggcctagg agacagttat gagcgcggat ctacagggag gggcgtcgtc
     1381 gtggagggcg tcgccgtggg tgcaactcgt atttggtgtg atttgcatgg caatgatcgc
     1441 gaacatgcag tacggctgga cgttgttcgt gaatccaatc aacgagaaat accaatgggg
     1501 tcgcactgcg atccaggttg cattcacgat ctttgtggtg acggaaacgt ggctcgtgcc
     1561 gatcgaaggc tatctcgtcg acaagtacgg tccgcgtccc gtcgtggtcg tcggcggcct
     1621 gctctgcgca gtggcatggg cgctcaactc ggtggcgtcg tcgctgccgc tgctgtatat
     1681 cgcggccgcc attggcggcg tcggcgcggg cgcagtgtac ggcacctgcg tgggcaacgc
     1741 gttgaagtgg tttcccaatc gccgcgggct tgccgcgggg ataaccgcag ccggttttgg
     1801 cgcagggtcg gcggccaccg tcgtgccgat tgccagcatg atcaagagca gcggctacga
     1861 agccacgttc ctctggttcg gtcttggcca gggtgtcatc gttttcatac tcggtctcgc
     1921 gctgctcgcg ccgccggcca ctctgctcgc gtcggcgaag agcgcggtgc agcgcgtggt
     1981 ctacaacgcg acgccacgcg aagtgattgg ctcgccggtg ttctgggtca tgtacctgat
     2041 gttcgtgatg atggctgcgg gcggcctgat ggcgaccgcg cagctcggac cgatcgcaaa
     2101 agacttcggt ctgcacgatt cgccggtttc gctgctcgga ttgaccttgc ccgcactgac
     2161 cttcgcgctg accatcgacc gcgtgctgaa cggtttgacc cggccgttct tcggctggat
     2221 ctc
//
LOCUS       DATASH010000965         7885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1748967,
            whole genome shotgun sequence.
ACCESSION   DATASH010000965 DATASH010000000
VERSION     DATASH010000965.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7885
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1748967"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /locus_tag="VHV99_25755"
     CDS             complement(<1..1038)
                     /locus_tag="VHV99_25755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX3638825.1"
                     /translation="MKSAFSFFPAWPLTPDAIFWAGLALLAAGLCGELCYRAWRLPRI
                     SGYAVIGLVAGSAGFGVIDADSASAARPLLDVALGLLLFELGSRLDLRWIRRNPWLIL
                     SSVAEATLTFALVLPVLLFLNVPLMVATVLAAIAMATSPAMVIQLKTELRAEGQVTQR
                     LLTLTALNSMYAVVIEKLVSSWLHQEIYGNVFATILQPLYLLVGSLVLAYLLARTCTF
                     FYRRLNMQDEHSFVALFGLVLLAIAVAHLFKLSTILALLAAGIIVKNHEARPQLWPQH
                     FGTAGWLLTVILFVLTLTSFEWKDIALGGVAALGLIVARLIAKLVGVMAFAKPSGLNW
                     KQGMALGLSLSP"
     gene            complement(1922..3889)
                     /gene="mnmC"
                     /locus_tag="VHV99_25760"
     CDS             complement(1922..3889)
                     /gene="mnmC"
                     /locus_tag="VHV99_25760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HEX3638826.1"
                     /translation="MTDPLIPAVLAFRDNGTPFSPLYDDIYHSAVGGLEQAQYVFLRG
                     NQLPERWQGRRVFTVLETGFGMGINFLTTWAAWRADPARCERLHFVSTEKHPFTQADL
                     RSVYAATISDPAIAALAKTLAGEWPMLVPGTHRLEFEGGRVTLTLVFADAMESLPALR
                     LRADAFYLDGFAPARNPDMWTPAIFKALARLAGEGATFATYSSAGDIKRALTQNGFVY
                     QKIDGFGWKRAMLVGHFAPRWRVRRYEPPAPLEVDERHAIVIGTGLAGCAAIERLASR
                     GWRVTSLERHASVAQDASGNPAGVFHPMISRDDSVASRVTRAGFLYALGRWAALERLG
                     HPPLRGARGLLQIAADDEEARSMSEAIAAFGYPSEYVTPVSLSEAQRLAGMPLAHSGW
                     LFPRGGWIDPASLCAAQCAAAGDLLERRFGVEVARLERSGNQWTAFDAEGQAVARAPV
                     VIVASAHDAARIAGLRHAPTRSIRGQLTLLPPGTVSPLRMPVMGVGYAVPLTDGVTLT
                     GATYELDDPDTSLRRDGHLENLKRVAQMLPPFDGIVDRTRAAALAGRVAFRCVTSDRM
                     PMIGNFGDEAASTRDAQRLRGAWPLDLPRVDGLYGAFAYGSRGLVWAALGAELIASQI
                     EGEPWPLERDLAEDIDPARFLLRALRQGTVS"
     gene            4306..4584
                     /locus_tag="VHV99_25765"
     CDS             4306..4584
                     /locus_tag="VHV99_25765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008352404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX3638827.1"
                     /translation="MNKQELIDAVAGQTGASKAQTGETLDTLLEVIKKSVSKGDAVQL
                     IGFGSFGSGKRAARTGRNPKTGETIKIPAAKTVKFTAGKAFKDAVNKR"
     gene            complement(4704..6014)
                     /locus_tag="VHV99_25770"
     CDS             complement(4704..6014)
                     /locus_tag="VHV99_25770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX3638828.1"
                     /translation="MNQPLLPVTVLSGFLGAGKTTLLNHILANRAGLRVAVIVNDLAA
                     VNIDATLVRDAAALSHVEQQLVELSNGCICCTLRDDLLVEIKRLAGEKRFDAILIEST
                     GVAEPMPIAETFTFVDDNGVSLSDVAQLDTMVTVVDAFNFLRDYASADALAERGIAAS
                     EEDDRTLVELLIEQIEFCDVLVVNKADLVSADELTRLQRILARLNPRAAQVVSRFGEV
                     PLAEVLNTGRFDFDEASSAPGWLASLNHEHDHEHEHGHAHHGEADEFGIGNFIYRARR
                     PFHPERLWTLLHQEWKGVLRSKGFFWLATRNDIAGSLSQAGGACRHGPAGMWWAAQER
                     SEWPNGDDELAAEIAADWFGDPDDLSIGDRRQELVMIGVGIDPATWQAKFDACLLTDA
                     EYATGPQAWQQFADPFPAWDFDEDDHDHHDHDGHGHGEIVHRHD"
     gene            complement(6257..6703)
                     /locus_tag="VHV99_25775"
     CDS             complement(6257..6703)
                     /locus_tag="VHV99_25775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX3638829.1"
                     /translation="MKRTFVTVAAGLAMLVAAGSARADCFDAAAGYQKVNPLILRAIA
                     WQESHNRPDALHKNANGSTDYGVMQINSVHLPTLAQYGISQGTLMEPCKNVYIAAWHL
                     RRQMNKYGNTWQAVGAYHSETPALRDKYAQQIAAILRKWNLMPAAR"
     gene            7232..>7885
                     /locus_tag="VHV99_25780"
     CDS             7232..>7885
                     /locus_tag="VHV99_25780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018432422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HEX3638830.1"
                     /translation="MALRRVATALLVAGLITAQTAQAQVTLNFVNADIDQVAKAIGAA
                     TGKTIIVDPRVKGQLNLVSENAVPEDQALKTLQSALRMQGFALVQDHGVLKVVPEADA
                     KLQGVPTYVGNAPVARGDQVVTQVFVLKNESANNLLPVLRPLISPNNTVAAYPANNSL
                     VVTDYADNVRRIAQIIAGVDTAAGQSVSVMPLKNANALDIAAQLTKMLDPGAIGSTDA
                     "
BASE COUNT         1673 a         2413 c         2426 g         1373 t
ORIGIN      
        1 cggcgataac gacaaaccga gcgccatccc ctgcttccag ttcaagccgc tcggcttggc
       61 aaaggccatc acgcccacca gtttcgcaat cagacgcgca acaatcagac ccagcgcggc
      121 cacgccgccc agcgcgatgt ccttccattc gaacgaagtc agcgtcagca cgaacagaat
      181 cacggtcaac aaccagccgg ccgtaccgaa atgctgtggc cacaactgcg gacgcgcttc
      241 atgattcttc acgatgatgc ccgcagccag cagcgcgaga atcgtcgaca gcttgaacag
      301 atgcgctacc gcaatggcca gcagcacgag gccgaacagc gcgacgaacg aatgctcgtc
      361 ctgcatgttc agacggcgat agaaaaacgt gcaggtgcgc gcgagcagat aagcgagcac
      421 gagcgagccg accagcaaat aaagcggctg cagaatcgtc gcgaaaacgt tgccgtaaat
      481 ttcctgatgc aaccagctcg agacgagctt ttcgatgacc accgcataca tgctgttcag
      541 cgcggtcaag gtcaacagac gctgggtcac ctggccttcc gcgcgcagtt cggttttgag
      601 ctggatcacc atggccggtg acgtcgccat ggcaatggcg gcaagcaccg tcgccaccat
      661 cagcggcaca ttcaggaaca acaaaaccgg cagaaccagc gcgaaagtca acgtggcttc
      721 ggcaacgctc gacagaatca gccagggatt gcggcgaatc cagcgcaaat cgagccggct
      781 acccagttcg aacaacaaca aaccgagcgc gacatcgagc aacggccgcg cggcgctcgc
      841 ggaatccgcg tcgattacgc caaaaccggc ggaacctgcg accagaccga tgaccgcata
      901 cccggagatg cgcggcaaac gccatgcgcg atagcaaagt tcaccgcaca ggccggcggc
      961 gagcagcgca agaccagccc aaaaaatggc gtcgggggtg agtggccacg ctggaaaaaa
     1021 tgaaaacgcc gacttcatcg tgatgggttc tccttgtcag caacggcaga cgacacgaac
     1081 cggcgcaaac accggcgagc gggtgggcac tcgaggcgct cactgtcgcg atcgcttcgc
     1141 gtggtccgcg ttgcgagttt tcaatgatcg gatgatctga acgcgccaca gccgcaggga
     1201 gcgcggcgcg ttcgggggtg cggccgtagc aaccggcccg gcacggtgaa agaacgccgt
     1261 cgactttgaa tcagattctg attgagcggc accgttccgt tgcagcgtgt gccgccagag
     1321 gcgaggcacg acgcggaaaa gaacggcgat tgtgccatag ctttttcagg cttgactgaa
     1381 aagacaggaa catgcaggta aaacggtaaa aacgtacgtt atcttcgcaa gatgaggaaa
     1441 aagcgaccgg tttaaaaccg ggtcgggctc tcatagggta cagattcgac gtggcagatg
     1501 tgtttcccag ggcacagcgg gaggtgtttt tctccgaact tttggcgatt cgagctcagc
     1561 gatcatgcgc gatctggcca cggcctttct gtttacaggt ttaccgtatg tatacgtagt
     1621 aacagttaac aagctgcctc gatttctgtg gataacccca gtttacccaa tcaattcatt
     1681 acgttgccac gcgcacaaca ggatgcacag catgtgcgtc aacggcgggt atacaagggt
     1741 aacttttcgc ggttttcggt ggccgcaaag ttaccccgtt tacgtccaca acagctccac
     1801 atgagttgcg ccggtaaaca gcgcggttat ccacaagttt cagtggataa ctcctgtcgc
     1861 gacaggcttg gtccgcaaag tttgtttccc ggcgcgttgg cccgatgtca gtctcgggcg
     1921 gttaactcac tgtgccttgg cgcaacgcgc gcaagagaaa gcgagccgga tcgatgtctt
     1981 cagccagatc ccgttcgagc ggccacggtt caccttcgat ctgcgaggca atcagctcgg
     2041 cgccaagcgc cgcccacacc agtccgcggg aaccgtaggc gaatgcaccg tacaagccgt
     2101 cgacacgcgg caaatcgagc ggccacgcgc cacgcaggcg ctgggcgtcg cgcgtcgatg
     2161 cggcctcgtc gccgaagttg ccgatcatcg gcatccggtc gctcgtcacg cagcgaaacg
     2221 ccacgcgccc ggcgagcgcc gctgctcgcg tccggtcgac gattccgtca aagggcggca
     2281 gcatctgcgc gacacgcttc aggttctcca gatggccatc tcgccgcagt gaggtgtcag
     2341 ggtcgtcgag ttcgtaggtg gcgccggtca gcgtgacacc gtcggttagc gggaccgcgt
     2401 agccaacgcc catcaccggc atgcggagcg gggatacagt gcccggcggc agcaaggtca
     2461 actggccgcg gatgctgcgg gtcggggcgt gccgcaaccc cgcgatacgc gcggcgtcgt
     2521 gggcgctcgc gacgatgacc accggcgcgc gcgcgacggc ctgcccttct gcgtcgaaag
     2581 cggtccattg attaccggac cgctcgaggc gcgccacctc cacgccgaaa cggcgttcga
     2641 gcagatcgcc cgccgccgcg cactgcgccg cacaaagcga tgccgggtcg atccagccgc
     2701 cgcgcgggaa gagccagccg ctgtgtgcga gcggcatgcc ggcaagcctt tgcgcttcgc
     2761 tcagcgatac gggcgtgacg tattcagacg gataaccgaa cgccgcgatt gcctcactca
     2821 tcgagcgggc ttcttcgtcg tccgcagcga tctgcaacag gccgcgagcg ccgcgtaatg
     2881 gagggtgacc aagccgttcg agcgctgccc agcgtccgag cgcgtagaga aagcccgcac
     2941 gcgtgacgcg cgacgcgacg ctgtcgtcgc gcgagatcat tggatggaac acgccggccg
     3001 ggttgcccga ggcgtcctgc gccaccgacg cgtgccgctc cagcgatgtg acgcgccaac
     3061 cgcgagaggc caggcgctcg atcgccgcgc agccggcgag cccggtgccg atcacgatcg
     3121 catgccgttc atcgacctca agcggcgcgg gcggctcata gcggcgcacg cgccagcgtg
     3181 gcgcgaaatg gccgaccagc atcgcgcgtt tccagccgaa gccgtcgatt ttttggtaca
     3241 caaagccgtt ttgcgtcaac gctcgcttga tgtcgccggc actgctgtag gtcgcgaatg
     3301 tggcaccctc gccggccaat cgagcgagcg ctttgaagat cgcgggcgtc cacatatcag
     3361 ggttcctggc cggcgcgaaa ccgtcgagat agaacgcatc agcgcgcagc ctcaacgctg
     3421 gcaggctttc catcgcgtcg gcgaagacga gcgtcagcgt gacccgtccg ccctcgaatt
     3481 cgagccggtg cgtgccgggg acgagcatcg gccattcacc ggccagcgtt ttcgccagtg
     3541 cggcaatcgc cggatctgaa atcgtcgccg catagacgct acgcaaatcg gcttgagtaa
     3601 acggatgctt ttcggtcgag acgaaatgca gccgctcgca acgcgccgga tcggctcgcc
     3661 aggcggccca ggttgtcagg aagttgatgc ccatgccgaa gccggtttcg agcacggtga
     3721 aaacgcggcg gccctgccag cgctccggca actgattgcc gcgaaggaac acgtattgag
     3781 cctgttcgag gccgccgaca gcgctgtgat aaatgtcgtc gtaaagcggc gaaaagggcg
     3841 tgccgttgtc gcgaaacgcg aggacggcgg ggatcagcgg atcggtcatg cgcagcggaa
     3901 aatgacgctc gaaagcgcga gtcagacgtt accgccagcg cgcgcaactc gccgggcggc
     3961 gaaaaagacg ggctgcaacg ttggcaaaaa ccaacgctaa gccccgccca tactgggttt
     4021 cagccctcga ttgaggctcg cgaagagaaa aaggaagggt ttcccctcaa tctcagcccc
     4081 ggaaccgctt aattcttcga aacccttatc ctgattgggt ttgcgctgcg ctatcatagc
     4141 aagcgccgcg tagggagcgg cggcacggcc gacgggcagt caaccgcccg gcgctaattg
     4201 tccggagggg atccggagcc gcagctgctc gacggcgcgg cccgcgcacg tataatcggc
     4261 gcgttcgcgc tgttcgtgtc aacctaaact cagaaaggaa ccttaatgaa caaacaggaa
     4321 ctgatcgacg ccgtcgcagg acagaccggc gccagcaagg ctcaaaccgg tgagacgctg
     4381 gacacgttgc ttgaagtgat caagaagtct gtgtcgaagg gtgacgcggt ccagttgatc
     4441 ggcttcggca gcttcggttc gggcaagcgc gcagcacgta cgggtcgtaa tcccaagacc
     4501 ggcgaaacca tcaagatccc ggcagccaag accgtcaagt tcacggctgg caaggcgttc
     4561 aaggacgccg tcaacaagcg ctaagcagat tgctgccagg cagttgacac ccgccaacgg
     4621 cgggtttttt ttcgtccgcg attttttgtg ctcgccgcga cgggcgccgc ggccagacaa
     4681 tcagctaaga gtggcaaggc cactcagtcg tggcgatgca cgatttcacc gtggccatga
     4741 ccgtcgtgat catgatggtc gtggtcatct tcgtcgaagt cccatgccgg gaacggatcg
     4801 gcgaattgtt gccacgcttg cgggcccgtc gcatattctg cgtcggtcag caggcacgcg
     4861 tcgaacttcg cttgccaggt agccgggtcg atgccgacgc cgatcatcac cagttcttgc
     4921 cgacgatcgc cgatgctcag atcgtccgga tcgccgaacc agtcggcggc aatttcagcg
     4981 gccagttcgt catcgccgtt cggccattcg ctgcgttcct gagcggccca ccacattccc
     5041 gccggaccat gccggcaagc gccgcccgcc tgcgacagcg aaccggcgat atcgttacgc
     5101 gtggcgagcc agaagaagcc cttgctgcgc aagacgccct tccactcctg atgcaggagc
     5161 gtccacaggc gctccggatg aaacggccgc cgcgcgcggt aaatgaaatt gccgatgccg
     5221 aattcgtccg cttcaccgtg atgcgcatgg ccgtgttcgt gctcgtggtc atgttcatga
     5281 ttgagcgacg ccagccagcc cggtgcgctc gatgcctcgt cgaaatcgaa gcgtccggta
     5341 ttcagcacct cggcaagcgg cacttcgcca aaacggctca ccacctgcgc ggcgcgcgga
     5401 ttgaggcggg cgagaatgcg ctgcaaacgc gtgagttcat cggcgctcac cagatcggcc
     5461 ttgtttacca ccagcacgtc gcagaattca atctgctcga tcagcaactc gaccagcgtc
     5521 cgatcgtcct cttcgctcgc ggcaatgccc cgctccgcga gcgcgtcggc tgaagcatag
     5581 tcgcgcagaa agttgaacgc atcgaccacc gtcaccatcg tatcgagctg cgcgacatcg
     5641 gacagcgaga cgccattgtc gtcgacaaag gtgaaggttt cggcaatcgg catcggctcg
     5701 gccaccccgg tcgactcgat caggatcgca tcgaagcgct tttcgcccgc cagccgcttg
     5761 atctcgacca gcaaatcgtc acgcaacgta cagcagatgc agccattcga cagttcgacg
     5821 agttgctgtt cgacatgcga gagcgcggcg gcatcgcgca cgagggttgc gtcgatattg
     5881 acggcggcca gatcgttgac gatcacggcg acgcgcaacc ccgcgcgatt cgcaaggatg
     5941 tggttcagga gcgtggtctt gccggcgccc agaaaaccgg agagcacggt gacgggcaat
     6001 agcggctggt tcatcgcagt acggaaaaac aagaggattg gaaggcgtgc cgggcttggg
     6061 tcgcatgact caggggccag aagatggccg aacgccgacg gcagcatgaa gccgcattgt
     6121 gcatcaaaca gggactggtc gcgtgacggg ccggatcaga aaatgcgagc agccatgctg
     6181 acaggcgcgg gctaaaaagc cgcatccgcg tgagtggcag cacctgtgcc gatgaaccta
     6241 tccacgaaaa taccgctcag cgtgcggccg gcatcagatt ccatttgcgc aggatcgcgg
     6301 cgatctgctg cgcgtattta tcgcgcagcg ccggcgtctc ggagtgatac gcgccgactg
     6361 cctgccaggt attgccatac ttgttcatct gacggcgcag atgccatgcc gcgatgtaga
     6421 cgttcttgca aggctccatc agcgtgccct gcgagatacc gtactgggcg agcgtgggca
     6481 gatgaaccga attgatctgc atcacgccgt agtcggtcga accgttggcg ttcttatgca
     6541 acgcatccgg ccgattatgc gactcctgcc acgcaattgc gcgcagaatc aagggattga
     6601 ctttctggta cccagctgcc gcgtcaaagc aatcggcgcg tgcggaccct gctgcaacca
     6661 gcatggcgag tcctgcggcg acagtgacga aggttctttt catcagtcca gatcggtcaa
     6721 gatgactaaa caaatcgcaa gggtaaggtt cggtaaggca atgcatcggc caaacttcag
     6781 ccgctggaag ccggtaaggc tcactggttg ggcactttgt cgcgcatcgg ggaaaacccg
     6841 cgttggcaag tcgcaaccag tacctcgaac ggctcgtatc ataccggcag gcctgcacgc
     6901 gtcaagttgg ctaaccgaca taatccagcg taaaacgaag cgtttccgtg gtccagcgca
     6961 cagaagctca tttgctttca attgggtgac gatccgtgac ggtcttttag agtaatcgca
     7021 cgtcgccgtt tgttttgtga caaaaacgac atgaaaagct gttactgttc atttttttcg
     7081 gacattggat ggctggcatc cggggcgttg acggcccgtg agggtggctg gcagaccgat
     7141 agggcggcgg cctgctcaaa tgttcgcatt gcatgaccgt cgacggctga ggccgtcggc
     7201 atcgatacca catttcatga gaagaaatcg tatggctttg cgtcgcgtcg caacggcgct
     7261 gctggtggct ggattgatca ccgcgcagac agcacaggca caggtgacac tcaacttcgt
     7321 gaatgccgac atcgatcagg tggccaaggc gatcggcgcg gcaaccggca aaacgatcat
     7381 cgtcgacccg cgcgtgaagg gtcagctcaa cctcgtgtcg gaaaatgcgg tgcctgaaga
     7441 tcaggcactc aagaccttgc aatccgcatt gcggatgcag ggcttcgcgc tggtgcagga
     7501 ccacggcgtg ctgaaggtcg tgcccgaggc tgatgccaag ctgcaaggcg tgccgactta
     7561 cgtcggcaat gcgccagtcg cgcgtggcga tcaggtggtc acgcaggtgt tcgtgctgaa
     7621 gaacgaatcg gccaacaacc tgctgccggt attgcgtccg ctgatctcgc cgaacaacac
     7681 agtggcggcc tacccggcca acaattcgct tgtcgtgacc gactacgccg acaacgtgcg
     7741 gcgcatcgcg cagatcatcg cgggtgtgga tacggctgcg ggccagtcgg tctcggtgat
     7801 gccgctgaag aacgcgaacg cgctcgatat cgccgcgcag ttgaccaaga tgctcgaccc
     7861 cggcgcgatc ggcagcaccg acgca
//
LOCUS       DATASH010000966         5510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2538860,
            whole genome shotgun sequence.
ACCESSION   DATASH010000966 DATASH010000000
VERSION     DATASH010000966.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5510
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2538860"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            252..983
                     /locus_tag="VHV99_25785"
     CDS             252..983
                     /locus_tag="VHV99_25785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175943.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX3638831.1"
                     /translation="MTKNTARALALALVCAAPAALALAPAMARAQSVTQYQPAGVLSL
                     NAQASAEVPQDVVDITLFYEQEASDPSALTSTLNQRADAALQKARSVSGVTARTGSFS
                     IYPSTDRDGRISAWRGRTEVVLESHDFAAVSKLAGQMASIMQVGNVQFSLSPETQRAA
                     EQKLTGEAIKSFREQAASSAQAFGYSGYSIREVNVGHNGVMPRPMMMMSARAMGADAK
                     ASAPVPLEGGTSTVTVNVSGSVQMK"
     gene            complement(1174..2079)
                     /gene="lgt"
                     /locus_tag="VHV99_25790"
     CDS             complement(1174..2079)
                     /gene="lgt"
                     /locus_tag="VHV99_25790"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX3638832.1"
                     /translation="MLIHPNFDPVAIHLGPLAVRWYGLMYLVAFIAAIVVGRLRLRLP
                     YVAAQGWTAKDIDDMLFYGVLGTILGGRLGYVLFYKASFYFAHPLDIFKVWEGGMSFH
                     GGFLGVTLAMVLFAYQRKRSWLQVTDFVAPMVPTGLAAGRLGNFINGELWGRVTDPSA
                     PWAMLFPGAAPDDAAWLTAHPQLAAQWHLNEVFAHYHMLPRHPSELYEIALEGVALFF
                     VLFFFSRKPKPMGAISAIFLIGYGLARFTVEFAREPDDYLGLLAMGLSMGQWLSLPMI
                     LAGIGMLVWSYRRARREPAQAVGAN"
     gene            complement(2213..3280)
                     /locus_tag="VHV99_25795"
     CDS             complement(2213..3280)
                     /locus_tag="VHV99_25795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3638833.1"
                     /translation="MLAPIPDLRQLRYFVTVAEEKHFGRAAARLSMTQPPLSQAVRAL
                     EETLGVVLFARTKRSVELTSVGADLLPEVQRLLASAEGLKPLAQSLARGEAGVLSLAF
                     VSTADYGLLPLLLRDFGARHPRVRLELTEATSDVQVDELVAGRIDAGLVIAPLPSRHA
                     TQLSWLPIAREPLVIAMSSDMAARLGGALGSDAGTNAKADAKADAKAATKPLTKPAAG
                     VHNNPHAEWLDTPISLRDVADEPLVIFPRRLAPGFYDIIMDCYGVAGVAPRVGQEAIQ
                     MQTIVSLVSAGMGVALVPQSLRNLRRTGVVYRPLSESVPAIETGLVWRTAEVSPVLAG
                     FIEIVRAHAAIAGAQYTAPRV"
     gene            3495..5168
                     /gene="ilvD"
                     /locus_tag="VHV99_25800"
     CDS             3495..5168
                     /gene="ilvD"
                     /locus_tag="VHV99_25800"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011694362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX3638834.1"
                     /translation="MAYNRRSKNITQGVARSPNRSMYYALGYQKEDFDKPMIGIANGH
                     STITPCNAGLQRLADAAVVAVKGADANPQIFGTPTISDGMSMGTEGMKYSLVSREVIA
                     DCVETCVQGQWMDGVVVIGGCDKNMPGGMIGILRANVPSIYVYGGTIRPGNWKGTDLT
                     IVSSFEAVGEFTAGRMSQEDFEGVEKNACPSTGSCGGMYTANTMSSSFEAMGMSLLYS
                     STMANPDQEKVDSAAESARVLVEAVKKDLKPRDIVTKKSIENAVAVIMATGGSTNAVL
                     HFLAIAHAAEVEWAIEDFERMRKKVPVICNLKPSGQFVATDLHKAGGIPQVMKILLDA
                     GLLHGDCLTITGKTIAEELKDVPSKPRADQQVIFPIEQALYKEGHLAILKGNLAVDGA
                     VAKITGLKNPVITGPARVFDDEQSALEAILADKVKAGDVVVLRYLGPKGGPGMPEMLA
                     PTSAIIGKGLGESVGLITDGRFSGGTWGMVVGHVAPEAFVGGTIAFVQEGDSITIDAH
                     KLLLQLNVDDAEIERRRAAWQAPKPRYTRGVLAKYSALALPANKGAVSG"
     gene            5370..>5510
                     /locus_tag="VHV99_25805"
     CDS             5370..>5510
                     /locus_tag="VHV99_25805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HEX3638835.1"
                     /translation="MKSMSHAMLAAAGVLSAVLGVGSPAAHAQAPAGLALAQQQNCMS
                     CHS"
BASE COUNT         1133 a         1737 c         1736 g          904 t
ORIGIN      
        1 gtgactgaag ccgaatggcg cttcatcacc aaacgtcttg tttgacggtg catacgcgca
       61 tacgacccgt tgtcgtttaa atggcgcttg aatcatgcgc aaatctgcgc gtgcatgaag
      121 gcaagcgttg agcaaacgtc aaattaggga accaagctaa ggttacaggc gcctaacgga
      181 ctcgcaaacg ttgtgccatc gcacggcgtt cgctccaatc gcgcaccact gttcaagcaa
      241 ggagtccagg aatgacgaaa aacaccgctc gtgcactcgc tctcgcgctc gtatgcgccg
      301 cccctgccgc gctcgcactc gcgccggcca tggcccgcgc gcagagcgtg acgcagtatc
      361 agcccgcggg cgtactgtcg ctgaatgcac aggcaagcgc ggaagtgccg caagacgtcg
      421 tcgacatcac gctgttctac gaacaggaag cgagcgatcc gtccgcgctg acctccacgc
      481 tgaatcaacg cgccgatgca gcgctgcaaa aagccaggag tgtgagcggt gttaccgcac
      541 gcaccggttc cttctcgatc tacccgtcca ccgatcgtga cggccgcatt tccgcatggc
      601 gcggtcgcac ggaagtcgtg ctcgaatcgc acgacttcgc cgcggtgtcg aaactcgcgg
      661 gccagatggc atcgatcatg caggtcggca atgtgcagtt ctcgctgtcg cctgaaacgc
      721 agcgtgctgc tgagcagaag ctcaccggtg aagccatcaa gtccttccgt gagcaggccg
      781 cttcgtcggc gcaggcgttc ggctatagcg gctattcgat tcgcgaagtc aacgttgggc
      841 acaacggcgt catgccgcgt ccgatgatga tgatgagtgc ccgtgccatg ggcgccgatg
      901 cgaaggcatc cgcgccggta ccgctcgaag gcggtacgtc gaccgtgacg gtcaacgtgt
      961 cgggctcggt gcagatgaag taacccgttc tacgctagat gtgcttgcgt tgaggcaagc
     1021 aaagtcaagt aaaaagccac ggcatgccgt ggcttttttc atgggctgcc cactacgata
     1081 aaacgtaacg ggcagttcgc gctaacgaac cgcgccagct ctgcggcgcg tggacaccac
     1141 ctggaacctt atccgtacga taaaccgccg cgctcagttc gcgccgaccg cttgcgccgg
     1201 ctcacgccgc gcacggcgat aggaccacac cagcatcccg atgcccgcaa ggatcatcgg
     1261 cagcgagagc cattggccca ttgagaggcc cattgcgagc aggccaaggt aatcgtccgg
     1321 ctcacgcgcg aattccacgg tgaagcgtgc gaggccgtag ccgatcagga atatcgcgga
     1381 aatcgcgccc atcggctttg gcttgcgcga aaagaagaac agcacgaaga acagcgcgac
     1441 accttcgagc gcgatctcat acaactccga cggatgacgc ggcagcatgt gatagtgcgc
     1501 gaacacttcg ttcaaatgcc actgcgcggc cagttgcgga tgcgcggtaa gccacgcggc
     1561 gtcgtccggc gcggcgcctg ggaacagcat cgcccacggc gccgacgggt cggtcacacg
     1621 gccccacaac tcgccgttga tgaagttgcc gaggcgccct gccgcgagcc cggtcggcac
     1681 catgggcgcg acgaaatcgg tgacctgcag ccacgagcgt ttgcgctgat acgcaaacag
     1741 caccatcgcc agcgtcacgc cgaggaagcc gccgtgaaac gacatgccgc cctcccacac
     1801 cttgaagatg tcgagcgggt gagcgaaata aaaactcgcc ttgtagaaca gcacatagcc
     1861 gagccggccg ccaagaatcg taccgagcac gccgtagaac agcatgtcgt cgatatcctt
     1921 cgcggtccag ccttgcgccg caacgtacgg caaacgcagc cgcagccggc cgacgacgat
     1981 cgccgcaatg aatgcaacga ggtacatcag tccataccag cgcacagcga gcggccccag
     2041 atgaatggca acggggtcga aattcgggtg aatgagcatc gtgttgtgtt atgggcagtt
     2101 caggttcaga agcggatttc taatggattt cgggtagcag ccagcggttt gcgcacatgc
     2161 ggcgcatgcg ttggacggtg cggctcgcaa tgcgttcgcg gccgcgccaa acctaaacac
     2221 gaggcgccgt gtactgcgcc ccggcgatgg ccgcatgcgc tcgcacgatc tcgatgaagc
     2281 cggccaacac cgggctcacc tccgccgtgc gccagaccag ccccgtctcg atggccggca
     2341 ccgattcgga gagcggccga tagacgacac cggtgcggcg caggttacgc agcgattgcg
     2401 gcaccagtgc gacacccatt ccggctgaca cgaggctcac gatcgtctgc atctggatcg
     2461 cctcctggcc gacccggggt gcgacgcccg ccacgccgta gcaatccata atgatgtcat
     2521 aaaagcctgg cgccagccgt cttgggaaga tcacgagcgg ctcatcggcg acgtcgcgca
     2581 ggctgatcgg cgtatcgagc cactcggcgt gtgggttgtt gtgcaccccg gcggcgggtt
     2641 ttgtaagggg ttttgttgcg gcttttgcgt cggcttttgc gtcggctttt gcgttggtcc
     2701 ccgcgtcaga ccctaacgcg ccgcccaaac gcgccgccat gtccgacgac atcgcgatca
     2761 ccagcggctc acgcgcgatc ggcagccatg acagctgcgt ggcgtggcgc gacggcagcg
     2821 gcgcaatgac gagccccgcg tcgatacgcc cggccaccag ttcgtcgacc tgcacgtcgc
     2881 tggtggcttc ggtcaattcg aggcgcaccc gcggatggcg cgcgccgaaa tcgcgcagca
     2941 gcagcggcag caggccgtaa tcggccgtcg agacgaaggc cagcgagagc acccccgcct
     3001 cgcctcgagc gaggctctgc gccaacggct tcagtccctc ggcgctcgcc agcaggcgct
     3061 gcacttcggg cagcaggtca gctcccaccg aagtgagctc caccgagcgc ttggtccgcg
     3121 cgaacaacac gacgccgagt gtctcctcca acgcccgaac cgcctgcgac aacggcggct
     3181 gcgtcatgga aaggcgcgcc gccgcgcggc cgaagtgctt ttcttcggcg acggtcacga
     3241 aatagcgcaa ttggcgcaaa tcaggaatgg gagcgagcat gattaatata ttttacgacc
     3301 taatacaggc taaataatat attggacaca cgtttcgcaa aactgcatcc ttgcgcgacg
     3361 cgtccgaact aaactggact gacgcgccgg ccgaagccgt cggcgacgca ttggcagcgc
     3421 agtcaccgcc tctgcggcag cactgagccc cgcatacgcg gcacgggcca aaacaaaaca
     3481 gattggagtt ccccatggca tacaaccgtc gctcgaaaaa catcacgcaa ggcgtggcgc
     3541 gttcaccgaa ccgctccatg tactacgcac tcggctatca gaaggaggac ttcgacaaac
     3601 cgatgatcgg catcgccaac ggccactcga ccatcacgcc gtgtaacgcc ggcctgcagc
     3661 gtctggccga cgcagcggtc gttgcggtca agggcgccga cgcgaatccg cagatcttcg
     3721 gcacgccgac gatttcggac ggcatgtcga tgggcaccga aggcatgaag tactcactgg
     3781 tgtcgcgcga agtcatcgcc gactgtgtcg agacgtgcgt gcaaggccag tggatggacg
     3841 gcgtcgtcgt gatcggcggt tgtgacaaga acatgccggg cggcatgatc ggcatcctgc
     3901 gcgccaacgt gccgagcatc tacgtgtacg gcggcacgat ccgcccgggc aactggaagg
     3961 gcaccgacct gaccatcgtg tcgtcgttcg aagcagtggg cgaattcacg gccggccgga
     4021 tgtcgcagga agatttcgaa ggggtcgaaa agaacgcgtg cccgtccacg ggttcgtgcg
     4081 gcggcatgta taccgccaac acgatgagtt cgtcgttcga ggcaatgggc atgtcgctgc
     4141 tgtactcgtc gacgatggcc aatccggatc aggaaaaggt cgactcggcc gccgaatcgg
     4201 cacgcgtgct ggtcgaggcg gtcaagaagg atctgaagcc gcgcgacatc gtgacgaaga
     4261 agtcgatcga aaacgcggtg gccgtgatca tggccaccgg cggttcgacc aacgccgtgc
     4321 tgcacttcct cgccatcgcg catgcggctg aagtggaatg ggccatcgaa gacttcgagc
     4381 gcatgcgcaa gaaagtgccg gtgatctgca acctgaagcc ctcgggccag ttcgtggcga
     4441 ccgatctgca taaggcaggc ggtattccgc aagtcatgaa gatcctgctg gacgcgggtc
     4501 tcctgcacgg cgattgcctc acgatcaccg gcaagacgat cgctgaagaa ctgaaggacg
     4561 tgccgagcaa gccgcgcgcc gatcagcagg tcattttccc gatcgaacag gcgctgtaca
     4621 aggaaggcca cctcgcgatc ctgaagggca acctggccgt cgacggcgcg gtggccaaga
     4681 tcacaggcct gaagaacccg gtcattaccg gcccggcccg cgtgttcgac gacgagcaaa
     4741 gcgcgctgga agcgattctc gccgacaagg tcaaggccgg cgacgtggtc gtgctgcgtt
     4801 acctcggccc gaagggcggc cccggcatgc cggaaatgct cgcgccgacc tcggcgatca
     4861 tcggcaaggg gctcggcgaa agcgtcggcc tgatcaccga cggccgcttc tcgggtggca
     4921 cgtggggcat ggtggtgggg cacgtcgcgc cggaagcgtt cgtgggcggc acgatcgcat
     4981 tcgtgcagga aggcgattcg atcaccatcg acgcgcacaa gctgctgttg cagttgaacg
     5041 tcgacgacgc cgaaatcgaa cgccgccgcg cggcatggca ggcgccgaag ccgcgttaca
     5101 cacgtggcgt gctggcgaag tactccgcgt tggcgctacc cgccaacaag ggcgcggtgt
     5161 cgggctgaac ggcatcggcg tgcatcgagc gcgcgcttcg attcgtctcc ggcaacgggg
     5221 cggatctgga gcaccgctcg aagctgcaca tgctacctga gctacgtaaa agcgcagaga
     5281 gtgcaaaggg gcgcacggga aaccgtgcgc ccctttgctt ttataatgcg tgcaccacga
     5341 gtcgggcact tgcaccggcg gagaccagaa tgaaatcaat gtcgcatgca atgcttgccg
     5401 cggctggtgt gctgagcgct gtgttaggcg tcggcagtcc ggcggcgcac gcccaggccc
     5461 ccgcgggcct cgcgcttgcc cagcagcaga actgcatgag ctgccactcg
//
LOCUS       DATASH010000967         3917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3131065,
            whole genome shotgun sequence.
ACCESSION   DATASH010000967 DATASH010000000
VERSION     DATASH010000967.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3131065"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1013
                     /locus_tag="VHV99_25810"
     CDS             <1..1013
                     /locus_tag="VHV99_25810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017330632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX3638836.1"
                     /translation="PIGVVACLIPFNYPVYTFMRKVAPALIAGNTVVVRPSNNTPSSA
                     FEIARAVENAGLPAGVVNILAMNHATAEAFCTHPKVGMITLTGSVGAGRKVLDYCKAN
                     IAKPSLELGGKTPAIIEADADLEKAARELVASKTTHCGQLCTAIERVYVQESVHDRFV
                     ALLKKHMCAVEFGDRSTQPSLMGPLVNEASRQLIHGMVERAIAAGATLETGGRLPQGK
                     GFFYPATLLSNCRQHMEIIQEETFGPIMPVVKYRTLDEALEMANDHQFGLSSVLYTEN
                     YRSAMKIANGIEAGELYVNRTPADPYHGFHAGWKRSGLGGDDGKHGMLEFTQTRLVIM
                     KY"
     gene            1163..2488
                     /locus_tag="VHV99_25815"
     CDS             1163..2488
                     /locus_tag="VHV99_25815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3638837.1"
                     /translation="MSSQPVQSTASLASSAAETALQSNKAQRYIQLMLLVLAAGAIYP
                     ILYLRQVYQPTMLEVFHITDSQLGYLYSSLGTIFLLSYLPSGWLADRIAPRLLICFSL
                     IATGALGLWYSTAPSFHVLMLIFGGWGVSTGLTFWAAVIKRVTMIAGTHEQGRFFGLL
                     DGGRGLIEAMLATIAITLFAWFTQTRGEPVAVGFKLVVYMYAFLCIALGVVLLLVKDP
                     QGVEDAAANRAAKKRSNVLVDLKTLASIPELWLVAAIVFCGYQVFWATYSFSAYLHEG
                     EIGLTVVMAGTITTLKLWMRPIGGIGGGFLGDRYSKVSVLVIALFLAALSLLGLMAAP
                     RISSHVLLVFLVLFIGILTYAIRGLYWSLLDRCNIPLETTGLAIGLISVLGYSPDVFL
                     PLINGYLTQNFPGVFGYQLYFGYVAGMATLGGFAGLALRNMLKRKELRK"
     gene            2485..3702
                     /locus_tag="VHV99_25820"
     CDS             2485..3702
                     /locus_tag="VHV99_25820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016969212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX3638838.1"
                     /translation="MKVVSLETHIVAVPPPHVGGMYWIFVRLKTDCGIEGVGEIYSAT
                     FHPKAMTHIIDDVFGRYLLDKDPHHIERLWREAYSSGFTQRPDLTMMGVVSGLEMACW
                     DIIGKAANKPVYALLGGMVNPRLRSYTYLYPKNSRGEYDYDDPDLAAECAVENVKRGF
                     TAVKFDPAGPYTAYSGHQLSMEVLDRCETFCRRVRDAVGSKADLLFGTHGQMVPSSAI
                     RLAKRLEKYDPLWFEEPVPPGQEGAMAQVARHTSIPIAAGERLTTKYEFFKLLEAGAA
                     SILQLNVARVGGLLEAKKVAAIAEVYYAQIAPHLYNGPIGAAASIQLAACTPNFLIQE
                     SIGTWDGFHAAVLKKPIQWEDGYIIPSQEPGLGVELNMDVVRQHTPYTGERLHLQMAA
                     QPADVKDLAPARG"
     gene            3804..>3917
                     /locus_tag="VHV99_25825"
     CDS             3804..>3917
                     /locus_tag="VHV99_25825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase family protein"
                     /protein_id="HEX3638839.1"
                     /translation="MNYDYIIVGAGSAGCILANRLSASGQHSVLLLEAGGKD"
BASE COUNT          738 a         1169 c         1220 g          790 t
ORIGIN      
        1 agccgatcgg cgtggtggcc tgcctgatcc cgttcaacta cccggtctat acgttcatgc
       61 gcaaggtcgc gcccgcgctg atcgcgggca acaccgtggt cgtgcggccg agcaacaaca
      121 cgccgagctc ggcgttcgaa atcgcgagag ccgtcgaaaa cgccggcctg cccgcgggcg
      181 tcgtcaatat tctggcgatg aaccacgcaa ccgccgaagc gttctgcacg catccgaagg
      241 ttggcatgat cacgctgaca ggcagcgtcg gcgcgggccg caaggtgctc gactactgca
      301 aggcgaacat tgcgaagccg tcgctcgaac tcggcggcaa gacgcccgcg atcatcgagg
      361 ccgacgctga tctcgagaag gccgcgcgcg aactcgttgc gtcgaagact acgcattgcg
      421 gccagctgtg caccgcgatc gaacgcgtgt acgtgcagga aagcgttcac gaccggttcg
      481 tcgcactgct gaagaaacac atgtgcgcgg tcgagttcgg cgatcgcagc acgcagcctt
      541 cattgatggg gccgctcgtc aacgaagcgt cgcgccagtt gattcacggc atggtcgagc
      601 gcgcgattgc cgcgggcgcc acgcttgaaa ccggcggcag gctgccgcaa ggcaagggct
      661 tcttctatcc ggctacgttg ttgtcgaact gccgtcagca catggaaatc atccaggaag
      721 aaaccttcgg cccgatcatg ccggtcgtca aataccgcac gctcgacgaa gccctggaga
      781 tggccaatga ccatcagttc ggtttgtcct cggtgctcta cacagagaac taccgcagtg
      841 cgatgaagat cgcgaacggc atcgaggcag gcgagttgta tgtgaaccgc acgcccgccg
      901 atccgtatca tggcttccac gcgggctgga agcgttcggg tctcggcggc gacgacggca
      961 agcacggcat gctcgaattc acgcagaccc gtctcgtgat catgaagtac taagcagagc
     1021 cggtgcgcgc cgtgccacgt aattgatcgc gatggcgcgc agctctttga aactcgcgaa
     1081 aacccgcggc cagcacgctg caaatccata acaaaacaga ttggagaccc ggcttggcgc
     1141 tgtgtctctc aggagcgctc acgtgtcatc tcaacccgtt caatccaccg cttcgctcgc
     1201 ctcgagcgcc gcggaaaccg cgttgcaaag caacaaggcg caacgctata tccagctcat
     1261 gttgctggtg ctggcggccg gcgcgatata cccgattctg tatctgcggc aggtctatca
     1321 gccgacgatg ctcgaagtgt ttcacatcac agatagccag cttggctatc tgtattcgtc
     1381 gctcggcacg atctttctgt tgagttatct gccgagcggc tggctcgccg accggatcgc
     1441 cccacgcctg ttgatctgtt tttcgctgat cgcaacgggc gcactaggcc tgtggtattc
     1501 gaccgcgccg tcgttccatg tgctgatgct gatcttcggt ggctgggggg tgtcgaccgg
     1561 tttgaccttc tgggctgccg tcatcaagcg cgtcacgatg atcgccggca cgcacgaaca
     1621 gggccgcttc tttggcttac tcgacggcgg acgcggcttg atcgaagcca tgcttgcgac
     1681 catcgcgatc acgctgttcg cgtggttcac gcagaccaga ggcgagccgg tcgcggtggg
     1741 tttcaagctg gtcgtctaca tgtacgcatt tctgtgcatc gcgctcggtg tggtgctctt
     1801 gctcgtcaaa gacccgcaag gcgttgaaga cgccgccgcc aaccgtgcag cgaagaaacg
     1861 cagcaacgtg ctggtcgatc tgaaaacgct cgcctcgatt cccgagttgt ggctggtcgc
     1921 ggccatcgtg ttctgcggct atcaggtgtt ctgggccact tacagctttt ccgcgtatct
     1981 gcatgaaggc gaaatcggct tgacggtggt gatggccggc acgatcacca cgctcaagct
     2041 gtggatgcgg ccgatcggcg gcattggcgg tggctttctc ggcgaccgct attcgaaggt
     2101 ctcggtgctg gtgattgcgc tcttcctcgc cgcactgtcg ctgctgggcc tgatggcggc
     2161 gccgcgtatc tcgagccacg tgctgctggt gttcctcgtg ctcttcatcg gcattctgac
     2221 ttacgcgatt cgcggtctgt actggtcgct gctggatcgc tgcaatatcc cgctcgaaac
     2281 gacgggcctc gccattggcc tgatctccgt actcggctat tcgcccgacg tgttcctgcc
     2341 gttgatcaac ggctatctga cgcagaactt cccgggcgtc tttggttacc agctttactt
     2401 cggctatgtg gcaggaatgg ccacgctcgg cggctttgcc ggcctcgcgc tgcgaaacat
     2461 gcttaaaaga aaggagttgc gtaaatgaaa gtcgtctcgc tggaaacgca tatcgtcgcc
     2521 gtaccgccgc cgcacgtggg cggcatgtac tggatcttcg tcaggctcaa gaccgattgc
     2581 ggtattgaag gcgtcggcga aatctattcg gcgacgtttc atccgaaggc gatgactcac
     2641 atcatcgacg atgtattcgg ccgctacctg ctcgataagg atccgcatca tatcgaacgg
     2701 ctctggcgcg aagcgtattc gagcggtttc acgcaacgcc ccgatctgac gatgatgggc
     2761 gtggtcagcg ggctggaaat ggcgtgctgg gacatcatcg gcaaagcggc gaacaaaccg
     2821 gtgtacgcgc tgctcggcgg catggtgaac ccgcgtctgc gctcgtacac gtacctgtat
     2881 ccgaagaaca gccgtggcga atacgactac gacgatcccg atctcgccgc cgaatgcgcg
     2941 gttgaaaacg tgaagcgcgg cttcaccgcg gtgaagttcg atccggcagg gccgtacacg
     3001 gcttattcgg gccatcaact gtcgatggaa gtgctcgacc gttgcgagac tttctgccgc
     3061 cgcgtgcgcg atgcggttgg cagcaaggcc gatctgctgt tcggcacgca cgggcagatg
     3121 gtgccgtcgt cggccatccg gctggccaaa cggctcgaga aatacgatcc gttgtggttc
     3181 gaagaaccgg tgccgccggg gcaggaaggc gcgatggcgc aagtcgcgcg gcacacgagt
     3241 attccgatcg ccgcgggcga gcgcctgacc accaagtatg agttcttcaa actgctcgaa
     3301 gcgggcgcgg cgtcgatcct gcaactgaat gtggcgcgcg tgggcggcct gctcgaagcg
     3361 aagaaagtcg cggcgatcgc cgaggtgtat tacgcgcaga tcgcaccgca cctgtacaac
     3421 gggccgatcg gcgcggctgc cagcattcag ctcgccgcct gcacgccgaa tttcctgatc
     3481 caggaaagta tcggcacgtg ggacggtttt catgcggcgg tgttgaagaa gccgattcaa
     3541 tgggaagacg gctacatcat tccttcgcag gagccgggct tgggcgttga attgaacatg
     3601 gacgtggttc ggcagcacac gccgtacacc ggcgagcgtc tgcatctgca aatggcggca
     3661 caaccggccg acgtgaaaga cctcgcgccg gccaggggtt gaaccgcgcc tgaacctgcc
     3721 gggttctgac gaagggttca ggctgcaagt ttcaggttgt ttcacgcatt caatggattg
     3781 ggcgccgtcg ggcgacatag agcatgaact acgattacat catcgtcggt gcgggttcgg
     3841 cgggctgcat tctcgccaat cgcctttcgg cgtcgggcca acattcggtg ctgctgctcg
     3901 aagcaggcgg caaggac
//
LOCUS       DATASH010000968         4042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6295306,
            whole genome shotgun sequence.
ACCESSION   DATASH010000968 DATASH010000000
VERSION     DATASH010000968.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6295306"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="VHV99_25830"
     CDS             <1..640
                     /locus_tag="VHV99_25830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HEX3638840.1"
                     /translation="HRRNAPAIGGMSALIPIKNDPAANDKAMGGVRSDKARDAGDGYD
                     GGWVAHPGLVPIAMEEFVKVLGDKPNQIGKQRDDVLVTATDLMDFRPEAPITEGGLRN
                     NINVGIHYLGSWLAGNGCVPIHNLMEDAATAEISRSQVWQWIRSPKGNLEDGRKVTVE
                     LVRELSAQELDKVKQAVGGDTKPYDRAAQIFEEMSTSERFTDFLTLPLYEEI"
     gene            complement(794..1195)
                     /locus_tag="VHV99_25835"
     CDS             complement(794..1195)
                     /locus_tag="VHV99_25835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HEX3638841.1"
                     /translation="MQTVFVYGTLRAGEVNDISEAAARNDIAAPNLLGTATVRGHLFD
                     FGSYPGLVVDEAGVDVTGDVYEIDDELVAVLDEIEAVYPGVEDRFLAREVMVKVDGNV
                     VNCRFYPVAPNAVKGLPEIRSGDWVEYRGTR"
     gene            complement(1278..1772)
                     /gene="rraA"
                     /locus_tag="VHV99_25840"
     CDS             complement(1278..1772)
                     /gene="rraA"
                     /locus_tag="VHV99_25840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HEX3638842.1"
                     /translation="MSFATADLCDAHEDQLTLGTLRVLEPVFHLFSRAECFSGEAVTL
                     KVFEDNALIRATLEEKGAGRVLVVDGGGSLRCALVGDNLAQIAEQNGWAGIVLNGCVR
                     DTLELNEVDVGVAALATCPRRGQTLGTGERDVPVQLPGALVRPGEWIYADIDGVLVAS
                     ASLN"
     gene            1930..2799
                     /locus_tag="VHV99_25845"
     CDS             1930..2799
                     /locus_tag="VHV99_25845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX3638843.1"
                     /translation="MNSSADSALSSQIRFVDIPPEFQPTQAHPIRVRSRLMPSGWRIA
                     RHTHAWAQVAYASRGVLRVATTGTTWMVPPSRAIWLPPHVTHEVVAVEEAFLRTLYIT
                     ESTVPAGLDTPRVVEVSDLLREVIAALDTPGISAAREQLLGALALDELTRSEPLPLSV
                     PMPNEKRLRALCEAVIADPTNGDSLEQWASSVGASTRTIARLFRQELGVSFSQWRQQA
                     ILARAIPLLSQGRPLSHVAQELGYQSQSAFSAMFRRAFGESPRAFIERGSEHRAESGS
                     RGETEADEETAQK"
     gene            complement(2832..3296)
                     /locus_tag="VHV99_25850"
     CDS             complement(2832..3296)
                     /locus_tag="VHV99_25850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3638844.1"
                     /translation="MFDPTEAPSPFYLRLASMDDFEFAEALTRNNMGGYYHRHHLVWR
                     GDLFLGSWRESENFILELDGKPIGVLRITQEGDSLHIRDVQIAEGHRRLGAGTYLLDM
                     SHQWARERGLRELQLRVFVDNPAARLYQRKGYKLTGPRLAQLGAIRHLARRV"
     gene            complement(3449..3525)
                     /locus_tag="VHV99_25855"
     tRNA            complement(3449..3525)
                     /locus_tag="VHV99_25855"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3489..3491),aa:Asp,seq:gtc)
     gene            complement(3621..3696)
                     /locus_tag="VHV99_25860"
     tRNA            complement(3621..3696)
                     /locus_tag="VHV99_25860"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3660..3662),aa:Glu,seq:ttc)
     gene            complement(3758..3834)
                     /locus_tag="VHV99_25865"
     tRNA            complement(3758..3834)
                     /locus_tag="VHV99_25865"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3798..3800),aa:Asp,seq:gtc)
     gene            complement(3929..4004)
                     /locus_tag="VHV99_25870"
     tRNA            complement(3929..4004)
                     /locus_tag="VHV99_25870"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3968..3970),aa:Glu,seq:ttc)
BASE COUNT          806 a         1274 c         1192 g          770 t
ORIGIN      
        1 ccatcgtcgc aatgcgccgg caatcggcgg catgagcgcg ctgatcccaa tcaagaacga
       61 tccggccgcc aacgacaagg ccatgggcgg tgtgcgctcc gataaggcgc gcgatgcggg
      121 ggatggttac gacggcggct gggtcgcgca tccgggcctc gtgccgatcg cgatggaaga
      181 attcgtcaag gtgctcggcg acaagccgaa ccagatcggc aagcaacgcg acgacgtgct
      241 cgtcacggcc accgacctga tggacttccg tccggaagcg ccgatcaccg aaggcggtct
      301 gcgcaacaac atcaacgtcg gcattcacta cctgggttca tggctcgcgg gcaatggctg
      361 cgtgccgatt cacaacctga tggaagacgc ggcgaccgcg gagatttccc gctcgcaggt
      421 gtggcagtgg attcgctcgc cgaagggcaa tctcgaagac ggccgcaagg tcacggtcga
      481 gctggtgcgc gagttgtcgg cacaagaact cgacaaggtg aaacaggccg tcggcggcga
      541 tacgaagccg tacgatcgcg ctgcacaaat cttcgaggaa atgtcgacgt cggagcggtt
      601 caccgacttc ctgacgctgc cgctgtacga ggaaatctga ggcatctgag cggcctcatg
      661 catcaacaat aacgacgacg taccgggctc gctttcacca gccacgcaag ataaaagagc
      721 ggcccggatc gcaaggctga ccgaccggac ggcccgacag gcgcatgcct gccgggctgc
      781 ttttttgacg ccctcagcgc gtgccgcgat actcgaccca atcgcccgac ctgatttccg
      841 gcaggccctt taccgcattg ggcgcgaccg gatagaagcg gcagttcacg acgttaccgt
      901 ctaccttcac catcacttcc cgcgcgagga aacgatcttc gacgccggga tataccgctt
      961 cgatctcatc gagcaccgca accagttcgt cgtcgatttc atagacgtcg ccggtgacat
     1021 ccacgcccgc ttcgtcgacc accagacccg gatacgagcc gaagtcgaac agatggccgc
     1081 gtaccgtggc cgtgccgagc agattcggcg cggcgatgtc gttgcgcgcc gcagcctcgc
     1141 tgatgtcgtt tacttcaccg gcccgcaatg tgccgtatac aaagacggtc tgcatcgtcg
     1201 tttgctctcc ttgataatgg gtttcagctt gcctcggcgg tagccggggt tcggtttgcc
     1261 cttgcagttc cgaaggttca gttcagcgac gcgctcgcca ccagcacgcc gtcgatatcc
     1321 gcatagatcc attcgccagg acgcactagc gcgcccggca attgcaccgg cacatcgcgc
     1381 tcgccggtgc cgagcgtctg gccgcgccgt gggcaggttg ccaacgccgc cacgccgacg
     1441 tcgacttcat tgagctccag cgtatcgcgc acgcagccgt tcagcacgat acccgcccag
     1501 ccgttctgct ccgcaatttg cgcgagatta tcgcctacca gcgcgcaacg cagactgcca
     1561 ccgccgtcga ccaccagcac ccggcccgcg cccttctcct cgagcgtggc gcgtatgagc
     1621 gcgttgtctt cgaacacctt tagtgtcacc gcttcgccgc tgaaacactc ggcgcgactg
     1681 aagagatgaa agaccggctc gagcacgcgc agcgtaccca gcgtgagttg gtcctcgtgc
     1741 gcgtcgcaca ggtcagcggt tgcaaagctc atgagttcat cctcgggatc cgtgggcaat
     1801 cgggacatta tgcacgttga cgctgatgta accacgcatg aaacgctcta tgcttgagca
     1861 tccgtgcacc tggtgccata tttgcagtca ccaccgtttt gagctctgcc cattggatcg
     1921 atcttcaaca tgaattcgtc cgctgactca gccctgtcga gccagatccg cttcgtcgac
     1981 atcccacccg aattccagcc gacgcaagcg catccgattc gcgtgcgctc gcggctgatg
     2041 ccgtcaggct ggcggatcgc gcggcacacg cacgcgtggg cccaggtggc ctatgcgtcg
     2101 cgcggcgtac tgcgagtcgc gacgaccggc acgacatgga tggttccgcc atcgagagcg
     2161 atctggctgc caccgcacgt gacacacgag gtggtcgcgg tcgaagaggc atttctgcgc
     2221 acgctctaca ttaccgagag caccgtcccg gccggactgg atacaccgcg cgtggtcgaa
     2281 gtatcggatc tgctgcgcga agtcatcgcc gcgctcgata cgcctggtat ttcagcggca
     2341 cgtgaacaac tgctaggcgc gcttgcactc gacgaactga cgcgttcaga gccgctgccc
     2401 ctgtccgtgc cgatgcccaa cgagaaacgc ttgcgcgcat tgtgcgaggc cgtgatcgcg
     2461 gacccgacga acggcgactc gctcgaacag tgggcctcga gcgtcggtgc gagtacgcgc
     2521 acgatcgcgc gcctgtttcg tcaggaactg ggcgtgagct tttcgcaatg gcgtcagcag
     2581 gccattctcg cgcgcgcgat tcccctcttg agccagggac ggccgctatc gcatgtcgcg
     2641 caggagttgg gctatcagag ccagagtgcg ttctcggcga tgttccggcg ggcattcggc
     2701 gaaagtccgc gcgcgttcat cgagcgtggc tctgaacatc gtgcggagag cgggagtcgt
     2761 ggtgaaacag aggccgacga agaaaccgcg caaaagtgac tgcgcgactc gtcaccgcta
     2821 gccgcggtgc gtcaaacgcg ccgcgccaga tgacggatcg ccccgagttg cgccagacgc
     2881 ggtccggtca gcttgtaccc tttgcgctga taaagccgcg cggcaggatt gtcgacgaac
     2941 acgcgcaact gcaattcgcg cagcccgcgc tcgcgtgccc actgatgcga catatcgagc
     3001 agatacgtgc ccgcgccaag ccgccgatgc ccttcggcga tctgtacgtc gcggatatgc
     3061 agcgagtcgc cttcctgggt gatgcgcagc acgccgatcg gcttgccgtc caactcgaga
     3121 atgaaatttt ccgactcgcg ccaactgccg agaaacagat cgccacgcca gaccagatgg
     3181 tgccggtgat aatagccacc catgttgttg cgagtcagcg cctcggcgaa ctcgaaatcg
     3241 tccatgctgg cgaggcgcag ataaaacggt gacggagctt cggttggatc gaacatggtg
     3301 gaacgacgaa gatgggtcgg gtcaacggta aaacaggccg cgcgcgctgg caatggctgt
     3361 ttgtcccgag catacccgga cgatatccag gcagaccggc tgcgcaaata aaaaagccca
     3421 cttctttcga agtgggcttt ctaaaatctg gcggagcgga cgggactcga acccgcgacc
     3481 cccggcgtga caggccggta ttctaaccaa ctgaactacc gctccagatt ttttgcaccg
     3541 cggcttgtga gctttgctca ttcaccaact gcaccgctca tcgcgctaac tcatattgca
     3601 actgagcgac accgatatcc tggcgtcccc taggggattc gaacccctgt actcaccgtg
     3661 aaagggtgat gtcctaggcc tctagacgaa ggggacaacg tactgcttac acctttaatg
     3721 aaaaagcccg ttcaagtttg catgaacggg ctttgtctgg cggagtggac gggactcgaa
     3781 cccgcgaccc ccggcgtgac aggccggtat tctaaccaac tgaactacca ctccagtttg
     3841 cctatcatga cttgcgagcg ttggtttttt ctctgcaagc tgcgttgctt tactgcttct
     3901 tatcgaacac taaagcgacg ctgatgtttg gcgtccccta ggggattcga acccctgtac
     3961 tcaccgtgaa agggtgatgt cctaggcctc tagacgaagg ggacataatc ttttcagatt
     4021 tgtctttatc ttgctgctaa ct
//
LOCUS       DATASH010000969         7810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1946597,
            whole genome shotgun sequence.
ACCESSION   DATASH010000969 DATASH010000000
VERSION     DATASH010000969.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7810
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1946597"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VHV99_25875"
     CDS             <1..588
                     /locus_tag="VHV99_25875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638845.1"
                     /translation="PQIAPESAGDLFDATEIDEILTLRILTMTDEEKREMIATDERAR
                     ALLARTEGLGAEQMQKLHGTLRQVRALDAASVGASISPTQTAVPVSPWAELDARPQLA
                     CVRVAGVEIRVGDQVRLRPRGRADIFDIALSGMVATIESIERDFDDHVHVAVTVDDDP
                     GKDFGMQRMPGHRFFFGPDEVEPLGHVAGARRGLV"
     gene            585..1136
                     /locus_tag="VHV99_25880"
     CDS             585..1136
                     /locus_tag="VHV99_25880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HEX3638846.1"
                     /translation="MSTILVAGIGNVFFGDDGFGVEVVRRLQEQLAAGELPAFAAGVN
                     VADFGIRGVDLCYALLDGVDSAILIDATQRGGAPGTLYVIEPSMEESGGTANEDPYVV
                     PILMSPHEMDPARVLQTVRMLGGGCEDIVVLGCEPQDFGCGGGEEGRMSLSRPVAAAV
                     EQAAEVVVRLVTARLDQRVARVA"
     gene            1323..3734
                     /gene="hypF"
                     /locus_tag="VHV99_25885"
     CDS             1323..3734
                     /gene="hypF"
                     /locus_tag="VHV99_25885"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HEX3638847.1"
                     /translation="MSTHAQPAGQGSTQHPALAGPVGEEIRVRGLVQGVGFRPTVWRL
                     AHACGVAGDVCNDCDGVLIHAWGDAAMLQRFIDSLSAECPPLARIDAIERHPLSEAAE
                     ASDFRIVPSVSGHVQTGVTPDAVICADCAMEIADPANRRYRYPFTNCTHCGPRLSIVQ
                     AIPYDRANTTMAPFVMCDACRAEYDNPADRRFHAQPVACPACGPRVWLESRDGARVDG
                     DDPCRAASQLLRHGAIVAIKGLGGFQLACHASDETAVARLRRLKRRERKPFALMAHDL
                     QAVRRYCVPSEAECALLASAGGPIVIMSADGAECVAPSVAPGIGTLGFMLPSTPLHRL
                     LMESIDSALVVTSGNTSDEPPCIDNADARARLGRIADVFLMHDRDISRRVDDSVARVV
                     HGTARIVRRARGYAPAPLLLPEGFAATPAVLAMGGELKNTFCLARDSQAIVSHHIGDL
                     EDALTYADYRRAVAQYLHLFEHEPQVVALDLHPEYLSRKIGYELAQTLRIPVVEVQHH
                     HAHLAACMAENGVAFDASPMLGVALDGLGYGDDGTLWGGEFMLADYRGYTRLGQFKPV
                     AMPGGARAVDEPWRNAYAHLRAAFDWYGFTRHYSGLDIQQFLSSRPRAALDSMIAQRV
                     NSPLASSAGRLFDAVAATLDVCRERVLYEGQAAIELEALVDQHTLRNDSDVHAYPFEL
                     TNTMPGGLRCIEPRPMWEALLGDLLRGTPVPVIAVRFHKGLAIAIVRMVECLADLVTG
                     SADARSVALSGGVFQNRVLFEQVATRLGEAGFRVLTHRQVPANDGGLSLGQAVIAAAQ
                     RRDGV"
     gene            3781..4056
                     /locus_tag="VHV99_25890"
     CDS             3781..4056
                     /locus_tag="VHV99_25890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011880075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HEX3638848.1"
                     /translation="MCLGIPGQIVEVTDAANDLALVDVGGVRRVINIAFVIDDDRPAS
                     ACIGQWVLVHVGFAMSRLDEQEAARTLALLHELGVAQGEMNELRSAP"
     gene            4088..4795
                     /locus_tag="VHV99_25895"
     CDS             4088..4795
                     /locus_tag="VHV99_25895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX3638849.1"
                     /translation="MPEHESLQALYPFLHGARRDADRLDAALLESVHRKAQESVDAKT
                     RFFEENAPEVVRVAHAIADVYRRDGRLFTMGNGGSSCDAAHIAVEFLHPVTTGRPALT
                     AINLVADMAMLTAVGNDVGFNHIFVRQLVAQARRGDALIGVSTSGNSENLLAAFAKAK
                     EMELLTVGLSGHDGGRMATSAALDHCLVVRSDSIHRVQETHVAIYHIVWDLVHTLLAD
                     DRGGLGASATAAAGGTP"
     gene            4792..6039
                     /gene="hypD"
                     /locus_tag="VHV99_25900"
     CDS             4792..6039
                     /gene="hypD"
                     /locus_tag="VHV99_25900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HEX3638850.1"
                     /translation="MKYVDEFRDPEKAKTLAREIRALVSRIERAKQRPLQIMEVCGGH
                     THTIFRYGIQQLLPDAVEFVHGPGCPVCVLPMGRVDDCVALAERPEVIFTTFGDAMRV
                     PGSKKSLLKAKAEGADVRMVYSPLDALKIAQLNPEREVIFFGLGFETTMPSTAMTVLQ
                     AHVRKVTNFSLFCNHITIIPTIKAILDSPDLHLDGFLGPGHVSMVIGTRPYDFIARHY
                     RKPITVAGFEPLDVLQSLWMVLKQIADSRCEVENQYGRIVPEDGNAQALAAVARVFET
                     REFFEWRGLGSIDHSGVRVREAFAGFDAERKFAVPNLKIADPKACQCGEVLKGVIKPH
                     QCKVFGTGCTPESPLGSLMVSSEGACAAYYNYGRIDMSRIEGRQRARQAATPAAACLG
                     AGGMSDTAALANTVPTPTLRGGL"
     gene            6036..7127
                     /gene="hypE"
                     /locus_tag="VHV99_25905"
     CDS             6036..7127
                     /gene="hypE"
                     /locus_tag="VHV99_25905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HEX3638851.1"
                     /translation="MNDRASTPIALGAPPRVRRVRDASVNLAHGSGGRAMRDLIEDVF
                     VSTFDNPALAALEDQAVFALGDLAAHGDRLAFTTDSYVVDPLFFPGGDIGTLAVSGTV
                     NDLAVCGATPLYLSCAVVIEEGFSVDLLRRVAASMHRVALEAGVAIVTGDTKVVERGC
                     ADKLFINTAGIGVVRRDVSISARNARAGDVVIVNGYLGDHGAAILVARQQLALEADIE
                     SDCCPLNGLIAAMLDACPQIHCLRDATRGGVATVLNEFAQSSNVTIRLDEAALPLREQ
                     VQGACEILGLDPLYLANEGKLVAIVPAEAAGRVLVAMRAHPAGREAAVIGTVEGNEAK
                     ANGLVVMQTVFGGQRIVDMLVGEQLPRIC"
     gene            7146..7475
                     /gene="hypA"
                     /locus_tag="VHV99_25910"
     CDS             7146..7475
                     /gene="hypA"
                     /locus_tag="VHV99_25910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HEX3638852.1"
                     /translation="MHELSIANSVVEICAEQAHGARVRCVTLEIGQLSAVMPEAIRFC
                     FDVCAKDTAVEGATLDIVVMPGEARCLACGATLAVDVPFGQCACGSENLELISGQQLQ
                     IRRMEVV"
     gene            7475..>7810
                     /locus_tag="VHV99_25915"
     CDS             7475..>7810
                     /locus_tag="VHV99_25915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638853.1"
                     /translation="MCTTCGCSNTRLATVTDLDAVKAQEDNAQSQSQLPSNREAAAVG
                     SSETAYRRVDEHLHASLTAHTPGHSHSHMHSRHESSGHAHHDGHSHGGDHAHSHAHEH
                     GHDDGHVQQH"
BASE COUNT         1327 a         2368 c         2710 g         1405 t
ORIGIN      
        1 ccgcagatcg cgcccgaaag cgccggcgat ctgttcgacg ccacggagat cgacgagatt
       61 ctcacgctgc gcattctcac catgacggac gaagaaaagc gcgaaatgat cgccaccgac
      121 gagcgcgctc gcgcgttgct cgcgcgcacc gaagggctcg gcgccgagca gatgcagaag
      181 ctgcacggca ccttgcggca ggtgcgcgcg ctcgacgcgg cgagcgtggg cgcgtccatt
      241 tctccgacgc agaccgccgt gcccgtttcg ccatgggccg aactcgacgc gcggccgcag
      301 cttgcctgcg tgcgggtcgc gggcgtggag atccgcgtcg gcgatcaggt gcgcttgcgt
      361 ccgcgcgggc gcgcggacat cttcgatatc gcgctgagcg gcatggtcgc gacgatcgaa
      421 tcgatcgaac gcgatttcga cgaccacgtg catgtcgccg tgaccgtcga cgacgatcca
      481 ggcaaggact tcggcatgca gcgcatgccg ggccatcggt tcttcttcgg gccggacgag
      541 gtcgagccgc tcggtcacgt tgccggcgcc cggcgggggc tcgtatgagc acgattctgg
      601 tggccggcat cggcaatgtg tttttcggcg acgacgggtt cggtgtcgaa gtggtgcggc
      661 gtttgcagga gcagctggcc gcgggtgaac taccggcatt cgccgcgggc gtgaacgtcg
      721 cggacttcgg cattcgaggc gtcgatctgt gttacgcgct gctcgacggc gtggacagcg
      781 cgatcctgat cgacgcgacg cagcgcggcg gcgcgcccgg cacgctgtat gtgatcgagc
      841 cgtccatgga agagtcgggc gggacggcga acgaagatcc ctacgtcgtg ccaatcctga
      901 tgtcgccgca cgagatggac ccggccaggg tgctgcaaac cgtgcggatg ctcggcggcg
      961 ggtgcgagga catcgtcgtg ctcggctgcg agccgcagga tttcggctgt ggcggcggtg
     1021 aagaaggacg catgagtttg agccggcccg tggcggccgc ggtggaacag gccgctgaag
     1081 tagtggtgcg gctcgtgacg gcgaggctgg accagcgcgt cgcgcgggtg gcctgacata
     1141 ccgggtgcgc ggataggcga tggatacggc gtgaaataac tgaagcggga gacatcatga
     1201 aaggattctt caagtacctc ttgttaccgg cgttgctggt cggcgtgttg gcgaatatga
     1261 aggacatcag gcgctatatc cgtatccgca acatgtaggc gacaacggcc agggagatgg
     1321 cgatgagcac gcatgcacag cccgccgggc agggatcgac gcaacatcct gcgctcgcgg
     1381 gtccggtcgg cgaggagatc cgcgtgcgcg gactggtgca gggcgtcggc ttccggccga
     1441 ccgtgtggcg gctggcgcat gcctgcggcg tggccggcga cgtgtgcaac gactgcgacg
     1501 gtgtgctgat tcacgcatgg ggcgacgcgg cgatgctgca gcgcttcatc gacagcctca
     1561 gcgccgagtg tccgccgctc gcgcggatcg acgcaatcga gcggcatcca ttgagcgagg
     1621 cggccgaggc gagcgacttt cgtatcgtgc cgagcgtgag cggccatgtg caaaccggcg
     1681 tcacgcccga cgcggtcatc tgcgccgact gcgcgatgga gatcgccgat ccggcgaacc
     1741 gccgctatcg ttatccgttc accaactgca cgcattgcgg ccccaggctt tcgatcgtgc
     1801 aggccattcc ctacgatcgc gccaatacga caatggcgcc gttcgtcatg tgcgacgcgt
     1861 gccgcgcgga atacgacaat cccgccgacc ggcgctttca cgcgcaaccg gttgcgtgcc
     1921 ccgcgtgcgg accgcgcgtc tggctggaga gccgcgacgg cgcgcgtgtc gatggcgacg
     1981 acccgtgccg tgcggcgagt caactgttgc gacacggtgc gatcgtcgcg atcaagggct
     2041 tgggcggttt ccaactggcc tgccatgcga gcgacgaaac cgcggtcgca cgtctgcggc
     2101 ggctgaaacg gcgcgagcgc aaacccttcg cgctgatggc gcacgatctg caagccgtgc
     2161 gccgctattg cgtgccgagc gaagccgaat gcgcgctgct ggcgagcgcc ggcggaccca
     2221 tcgtgatcat gtctgccgac ggcgccgaat gcgtcgcgcc gagcgttgcg ccgggcatcg
     2281 gcacgttggg cttcatgctg ccatccacgc cgctgcaccg gctgctgatg gagtcgatcg
     2341 attcggcact ggtcgtgacg agcggcaaca ccagcgacga gccgccgtgc atcgacaacg
     2401 cggacgctcg cgcgcggctc ggccgtatcg ccgatgtctt tctgatgcac gatcgcgaca
     2461 tttcgcgccg ggtcgacgat tcggtggcgc gggtggtgca cggtacggcg cggatcgtgc
     2521 ggcgcgcgcg cggctatgcg ccggcccccc tgctgttgcc tgaaggtttc gccgccaccc
     2581 ccgccgtgct ggcgatgggc ggcgaactca agaacacttt ctgccttgcg cgcgattcac
     2641 aggcaatcgt ctcgcatcac atcggcgatc tggaagacgc gctgacgtat gcggactatc
     2701 gccgcgcggt ggcgcagtat ctgcatctgt tcgagcatga accgcaggtg gtcgcgctcg
     2761 atctgcatcc cgagtacctg tcgcgcaaga tcggctacga gctcgcgcaa accttgagga
     2821 ttcccgtggt cgaggttcag catcatcacg cgcatctcgc cgcgtgcatg gcagaaaacg
     2881 gcgtcgcctt cgatgcatcg ccgatgctcg gcgtcgcgct cgacggcctg ggttacggcg
     2941 acgacggcac gctgtggggc ggcgaattca tgctggccga ctaccgcggc tatacgcggc
     3001 tcgggcagtt caagcccgtg gcgatgcccg gcggcgcgcg cgccgtcgac gagccgtggc
     3061 gcaatgccta cgcgcatttg cgggcggcct tcgactggta cggatttacg cggcattaca
     3121 gcggtctcga catacagcag ttcctgagca gccgtccgcg cgccgcgctc gactcgatga
     3181 tcgcgcagcg cgtgaacagc ccgctggcga gttccgcggg ccgtttgttc gacgcggtag
     3241 cggccacgct cgacgtgtgc cgcgagcgtg tgctttacga aggccaggct gctatcgaac
     3301 tggaggcgct ggtcgatcaa cacacgctgc gcaacgatag cgacgtccac gcgtacccgt
     3361 tcgaattgac taacaccatg ccgggtggtt tgcgctgcat cgaaccccgg ccgatgtggg
     3421 aagccttgct aggcgaccta ctgcgcggca cgccggtgcc ggtgattgcc gtgcgttttc
     3481 acaagggcct ggcgattgcg attgtgcgca tggtggagtg tcttgccgat ctggtgacgg
     3541 gctccgcaga cgcgcgcagc gttgcgcttt cgggcggtgt gttccagaac cgtgtcctgt
     3601 tcgaacaggt tgcgacgcgg cttggcgaag caggttttcg cgtgctgacg caccgccagg
     3661 tgccggccaa cgacggcggt ttgtcgctcg ggcaggcggt catcgcggcg gcgcagcggc
     3721 gcgatggggt ttgaagcgtg cgtggagtag cggtgcagaa ccgggtatgg ggagagcgac
     3781 atgtgcttgg gcatcccagg gcagatcgtc gaggtgacgg atgcggcgaa cgatctcgcg
     3841 ctcgtcgacg taggcggtgt gcggcgcgtg atcaacatcg cgtttgtgat cgacgatgac
     3901 cggccggcga gcgcgtgcat cggccagtgg gtgctcgtgc atgtcggctt cgcgatgagc
     3961 cggctcgacg agcaggaagc ggcgcgcacg ctcgcgctgc tgcacgagtt aggcgtcgcc
     4021 cagggcgaga tgaatgaact gcgcagcgcg ccgtgagcgc gccaacatca gcatacggag
     4081 acccgccatg cctgaacacg aatcgctgca agcgctctat ccgttcctgc atggcgcccg
     4141 tcgggatgcg gaccggctcg acgccgcgtt gctcgaatcg gtgcatcgca aggcgcagga
     4201 aagcgtcgat gcgaaaacgc gcttcttcga agaaaacgcg cccgaggtcg tccgcgttgc
     4261 tcacgccatt gctgacgtgt atcgccgcga tggccggctc ttcacgatgg gcaacggtgg
     4321 ttcgagctgt gacgcggcgc atatcgccgt cgagtttctg catcccgtga cgacgggacg
     4381 ccccgcactc accgccatca atctcgtcgc cgacatggcc atgttgacgg cagtcggtaa
     4441 cgacgtcggc ttcaatcaca ttttcgtacg gcaactggtc gcccaggcgc ggcgcggcga
     4501 tgcactgatc ggcgtgtcga cgagcggcaa ttcggagaac ctgctggccg ccttcgcgaa
     4561 ggcgaaagag atggaactgc tgaccgtcgg actatccggt cacgacggcg gacgcatggc
     4621 cacgagcgcc gcgctcgatc attgcctggt ggtgagaagc gacagtatcc atcgtgtgca
     4681 ggagacccac gtcgcgattt atcacattgt atgggacctc gtgcatacgc tgctcgccga
     4741 cgaccgtggc ggcctcggcg cttcggccac ggcggctgcg ggaggcacgc catgaaatac
     4801 gtcgatgagt ttcgcgaccc ggagaaggcg aaaaccctcg ccagggaaat ccgcgcgctg
     4861 gtgtcgcgca tcgagcgcgc gaagcagcgg ccactgcaaa tcatggaagt ttgcggcggc
     4921 cacacccata cgattttccg ctacggcatc cagcaattgc tgcccgacgc cgtggagttc
     4981 gttcacggcc ccggctgccc ggtatgcgtg ctgccgatgg gccgcgtcga cgattgcgtc
     5041 gctttggcgg aacgcccgga agtgatcttc acgacgttcg gtgacgcgat gcgtgtgccc
     5101 ggttcgaaga agagcctgct gaaggcaaaa gccgagggcg ccgacgtgcg catggtctat
     5161 tcgccgctcg atgctttgaa gatcgcgcag ctcaatcccg aacgggaggt catcttcttc
     5221 ggcctgggct tcgagacgac catgccgagc accgcaatga ccgtcttgca ggcgcacgtg
     5281 cgaaaggtca cgaatttctc gctgttctgc aatcacatta cgatcattcc gaccatcaag
     5341 gcgattctag attcgccaga cctgcacctc gacggctttc tcggcccggg acatgtcagc
     5401 atggtgatcg gcacgcggcc ttatgacttc atcgcgcgtc actatcgcaa gccgatcacg
     5461 gtggcggggt tcgagccgct cgacgtgctg caatcgctgt ggatggtgct gaaacagatc
     5521 gcggacagcc gctgcgaagt tgagaaccag tatgggcgga tcgtgcctga ggatggcaat
     5581 gcgcaggccc tggcggcggt ggcgcgcgtg ttcgagacgc gtgagttctt cgaatggcgt
     5641 ggactgggct cgattgatca ttccggtgtg cgggtgcgcg aggcgttcgc gggattcgat
     5701 gccgagcgca agttcgcggt gccgaacctg aagatcgccg accccaaggc atgtcaatgc
     5761 ggcgaggttc tcaagggtgt gatcaagccg catcaatgca aggtgttcgg caccggctgt
     5821 acgcccgaat cgccgctcgg ttctttgatg gtttcgagcg agggcgcgtg tgcggcgtat
     5881 tacaactacg gacggatcga tatgtctcgc attgagggac ggcaacgcgc gcggcaagcg
     5941 gcgacgccgg ccgcggcatg tctgggcgcg ggtggcatgt ccgatacggc cgcgctggct
     6001 aacacagtcc ccacgcccac cctgcgaggc gggctatgaa cgatcgcgct tccacgccaa
     6061 tcgcactggg cgcgccgccg cgcgtgcgcc gcgtgcgtga cgcttcggtc aatcttgcgc
     6121 atggcagcgg cggccgagcg atgcgcgatc tgatcgaaga cgtattcgtc tcgaccttcg
     6181 acaatcccgc gctcgcggcg ctcgaagatc aggccgtgtt cgcgcttggc gatctcgccg
     6241 cgcacggcga ccggctcgcc tttacgacgg atagctacgt ggtcgacccg cttttctttc
     6301 cgggcggtga catcggcacg ctggccgtgt ccggcaccgt caatgacctg gcggtgtgcg
     6361 gcgcgacgcc tttgtatctg tcgtgcgcgg tggtgatcga ggaaggtttc tctgtcgatc
     6421 ttctgcgccg tgtcgcggcg agcatgcacc gtgttgcgct cgaggctggt gtcgcgatcg
     6481 tcacgggcga cacgaaagta gtggaacgcg gttgcgccga caagctgttc atcaacacgg
     6541 caggcattgg cgtggtacgc cgcgatgttt cgatttcggc gcgcaacgcg cgggccggcg
     6601 acgtggtgat cgtcaacggg tacctcggcg atcacggcgc ggcaattctg gtggcgcgtc
     6661 aacagttagc actcgaagct gacatagaga gcgattgttg tcccctcaac ggcctgatcg
     6721 cggcgatgct cgacgcgtgc ccacagatcc attgtctgcg cgatgcgacg cgcggcggtg
     6781 tcgcgaccgt gctcaacgaa ttcgcgcaaa gttcgaacgt gacgatacgg ctcgatgaag
     6841 ccgcgctgcc cttgcgcgag caggtgcagg gcgcctgcga gatcctcggg ctcgatccgc
     6901 tgtatctcgc caatgaaggc aagctggtgg ccatcgtgcc cgccgaggcc gccggacgcg
     6961 tgctggtagc catgcgtgcg cacccggcgg gacgcgaggc cgcggtgatc ggcacggtcg
     7021 aggggaacga ggcgaaggcg aacggactgg tcgttatgca gacggtgttc ggcgggcagc
     7081 gcatcgtcga tatgctggtg ggcgagcaac tgccgcgcat ctgctaaagg ttcgagggaa
     7141 gcgacatgca tgaattgagt atcgcgaaca gcgtcgtcga gatttgcgcg gaacaggcgc
     7201 acggtgcgcg tgtgcggtgc gtgacgttgg agattgggca gttgtcggca gtgatgccgg
     7261 aggcgatccg gttttgcttc gatgtatgcg cgaaagacac ggccgtggag ggggcgacgc
     7321 tggatatcgt cgtgatgccc ggcgaggcac gctgtctggc gtgcggtgcg acgctcgcgg
     7381 ttgacgtgcc gtttgggcaa tgcgcgtgcg gcagcgagaa cctcgaactg atttccggcc
     7441 agcagttgca gattcgacgg atggaggtgg tgtgatgtgt acgacatgcg gatgctcgaa
     7501 tacgcggctt gcgacggtga ccgatctgga tgcggtgaag gcccaggaag acaatgccca
     7561 gtcgcagtcg caattgccat cgaaccggga agccgcggca gtcggctcgt ccgaaactgc
     7621 ctatcggcga gtcgacgaac atcttcatgc aagtttgact gcgcacacgc ccggtcattc
     7681 gcattcgcac atgcatagcc gtcacgagtc gagcggacac gcacatcacg atggacattc
     7741 gcatggcggt gaccatgcgc attctcacgc gcatgaacac ggtcacgacg atggacatgt
     7801 ccagcaacac
//
LOCUS       DATASH010000970         5160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1946621,
            whole genome shotgun sequence.
ACCESSION   DATASH010000970 DATASH010000000
VERSION     DATASH010000970.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5160
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1946621"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1800)
                     /locus_tag="VHV99_25920"
     CDS             complement(<1..1800)
                     /locus_tag="VHV99_25920"
                     /inference="COORDINATES: protein motif:HMM:NF024739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX3638854.1"
                     /translation="MSLIDHQRRKLLQLMGAAGAVGLSLGSPLAMAASESLAATTFPG
                     AWEESQRKILLPAFRKAAGTSAVLTASQAVDTLTKLVAARNNPPFDVVMMDEGPYLEG
                     VAQGVFEPIPAAKVPNLANLPAHFIDPKGLGAYVSAQVYGIAYNTERVKTTPKSWNDL
                     LKPEFKGRVGLVSLDSTLGTVWMVALAKMLGGDEDHMDPAFDYIKRLMPNVGAVAANP
                     GALGTLFQQGQVDIACHYINNVESLKAKGVPVALTRPDSNWGIVRSTMNIVKNTRAAD
                     LAAAYINTALSVEVQQQMTSAPYYVAPTNTKVPFGAALAAIAHNANDMTSFVQVDWAR
                     INPRRAEYIDRFNRLVKVLRLMNANHRMPRDWLLVLPLGLFFIAFVGVPLLLVALISL
                     HNDTAMTHFGIRQYMVFFGDSYNRGILWQTLWLGVATTLVTIVLGYPVAHLYVVSPPR
                     WRRVLMVLILLPLLTSSVVRTFAWVVILGNEGLVNTLLMALGHAGPPIKLLYTPLAVI
                     LSLAQIELPLMVLPLVTTLTSLDTRLPEASHALGASSWRTWRTITVPLAMPGLLSGTL
                     LVFTGAVSAFVVQTLVGGGQLMYMPFYIYEQAIQ"
     gene            complement(1887..3074)
                     /locus_tag="VHV99_25925"
     CDS             complement(1887..3074)
                     /locus_tag="VHV99_25925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3638855.1"
                     /translation="MSTPHSIDSQSKHGTARAGLAAAQARAAQTGAAVQMRGIRHRFA
                     AVPVIEDVTLEIAPGELVALLGPSGCGKSTLLRILAGLQVQSEGEVRVGGQRVDGLAP
                     RQRGVGIVFQNYALFPHMTVAANVAYGLEANGVARRDAARKATQMLEMVRMEAFAKRY
                     PRELSGGQQQRVALARTLAVEPRILLLDEPFAALDKNLRLDMQIEIRRLQRELNITTV
                     MVTHDQEEAMSMADRVAVLNRGRLEQYDSAVAIYDQPATPFVATFVGTANLLPVTLRV
                     HGDQCLANFDDGAVLAVAQPGPWRDAGAALLAVRPEQWAWQLPQPGAGAPQLLATVQL
                     AMPIGPTLMVDLQTDAGPAVKISVPRAEGAGLRPGLRVALDFKPGARIRTFKAPQPGT
                     AGV"
     gene            complement(3071..4429)
                     /locus_tag="VHV99_25930"
     CDS             complement(3071..4429)
                     /locus_tag="VHV99_25930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3638856.1"
                     /translation="MTALPIEFDFDLLLLGATVVTMDPAQPLLHDAAIGIRGNRLAFV
                     GRAQDLPVQMRARSTRRLAGRVIVPGYVNVHTHAILTMVRGVAEDLGFAPAYTPGIPH
                     GHDVSPDEAIALARLGALEAMLFGSTLINDTYVHADLTMEAMADLGLRVYSCGRLHDV
                     DFSGVADGRWEYHAATGERTLNDALTLAGRWNRKPNGRTGVQLAVHAPDTCSDDFLRL
                     IAEAARTHDLGVTTHLAQSRTEVSVVKKRSGKSPAQLLDEVGLLNERLVAAHCLYLDD
                     DDIARVGRAGITVAHIPKGNATGGTMAPTPRLRAAGARIALGTDNMHADMTEVMRWAL
                     AIARIQLGEVAADWQPADALAMATHNGAHAMGLGDELGCLKTGWLADLAIFDFQRAHL
                     TPHPNPLGTLVHTGLGRDVEMVIVDGEIVVDGGAPTRCDADAVIAAGKHAATQLWKRA
                     SA"
     gene            complement(4452..5153)
                     /locus_tag="VHV99_25935"
     CDS             complement(4452..5153)
                     /locus_tag="VHV99_25935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX3638857.1"
                     /translation="MLPSVSLQVEHGETVVLIGPSGSGKTTLLRCLNFLERPDGGTIR
                     LGGERIGLSEQGRTLPEAELARQRSRIGFVFQRFNLFAHLSALDNVAAGPQRVLGLSR
                     DAARARAQLALEKVHLAQHLGKRPAQLSGGQQQRVAIARALAMEPEMILFDEPTSALD
                     PELVNEVLDVMRDLATARMTMMVVTHEMSFARNVAHRVVFMDAGAIVEQGPPGDVFGR
                     PREERTRRFLQHLHT"
BASE COUNT          911 a         1648 c         1730 g          871 t
ORIGIN      
        1 ctggatggcc tgttcgtaga tgtagaacgg catgtacatc agctggccgc cgcccaccag
       61 ggtttgaacc acgaacgcgc tgaccgcccc ggtaaacacc agcaaggtac cgctgagcaa
      121 acccggcatc gcaagcggca cggtgatcgt tctccacgtg cgccagctgc ttgcccccag
      181 cgcgtgcgac gcctcgggca agcgcgtgtc gagcgaggtg agggtggtga cgagcggcaa
      241 caccatcaac ggcagctcga tctgcgcaag cgagagaatc accgccagcg gcgtgtacag
      301 gagcttgatc ggcgggcccg cgtgtccgag cgccatcagc agcgtgttga cgagcccttc
      361 attgccgaga atcacgaccc aggcaaacgt gcgcaccacc gaactggtca gaagcggcag
      421 caggatcagc accatcagca cgcgccgcca gcgcggcggg ctcaccacgt acaggtgcgc
      481 gaccggataa ccgagcacga tcgtcaccag cgtggtcgcc acaccgagcc agagcgtttg
      541 ccagaggatg ccgcggttat aactgtcgcc aaagaacacc atgtattggc gaatgccgaa
      601 atgcgtcatg gctgtgtcgt tgtgcaggct gatcagcgcg acgagcagga gcggcacgcc
      661 cacaaacgcg atgaagaaca ggcccagcgg caacactagc agccagtcgc gcggcatccg
      721 gtgattcgcg ttcatgaggc gtaagacctt gaccagacgg ttgaaacggt cgatgtattc
      781 cgcgcggcgc ggattgatac gtgcccaatc aacctgcacg aacgacgtca tgtcattggc
      841 gttgtgagcg atcgcggcga gcgctgcacc gaacggcact ttggtgttgg tcggcgcaac
      901 gtagtagggc gcgctcgtca tctgttgctg cacttcgaca ctcaacgcgg tgttgatata
      961 cgccgcggcg agatccgccg cccgggtgtt cttcacgatg ttcatcgtgc tgcgcacgat
     1021 gccccaattg ctatcggggc gcgtgagcgc aaccggcacg cccttggcct tgagcgattc
     1081 cacgttgttg atgtagtggc acgcgatatc cacctgacct tgctggaaca gcgtaccgag
     1141 cgcgcctgga ttggcggcca ccgcgccgac gttcggcatc agacgcttga tgtaatcgaa
     1201 cgccgggtcc atgtgatctt cgtcgccgcc cagcatcttg gcgagcgcca ccatccatac
     1261 ggtgcctagc gtggaatcga gactcacgag gccgacgcgg cccttgaatt ccggcttgag
     1321 cagatcgttc caggatttcg gcgtggtctt cacacgctcc gtgttgtacg caatgccgta
     1381 gacctgcgcg gacacatagg cgcccagacc tttcggatcg atgaagtgcg cgggcagatt
     1441 cgccaggttc ggcaccttgg ccgccggaat gggttcgaac acgccttgcg cgacgccctc
     1501 caggtagggg ccctcgtcca tcatgaccac gtcgaacggc gggttgttgc gcgcggccac
     1561 cagcttggtc agcgtgtcga ccgcttgcga ggcggtgagc acggcactcg tgcccgcggc
     1621 cttgcgaaac gcgggcaaca ggatcttgcg ttgcgattct tcccacgcac cggggaacgt
     1681 ggtggcggcg agggattcgg acgcagccat ggcaagcggg ctgccgagcg acaggccgac
     1741 ggcccccgct gcgcccatca gttgcaggag cttgcggcgt tggtgatcga tcagcgacat
     1801 aggacctcag gaaggatagt tgaacaggat gaacgggcga tgctgaagcg ggcggcgcgt
     1861 tgaatcagga cgatcgtgac catgcgctaa acccctgcgg taccgggttg gggcgccttg
     1921 aatgtccgta tccgtgcacc cggcttgaaa tcgagcgcga cgcgcaagcc ggggcgcaaa
     1981 ccggcaccct cggcgcgcgg cacggaaatc ttcacggccg gtccggcatc ggtctgcaga
     2041 tcgaccatta gcgtggggcc gatcggcatc gccagttgca cggtagccag caactgaggc
     2101 gcgccggcgc cgggctgcgg caactgccac gcccattgct cggggcgaac cgccagcagc
     2161 gccgcgccgg catcgcgcca cgggccgggc tgcgccacgg ccagcacggc gccatcgtcg
     2221 aagttggcaa ggcactggtc gccgtgcacg cgcaaggtga ccggcagcag attcgccgtg
     2281 ccgacgaagg tggcgacgaa gggcgtggcg ggctgatcgt agatcgcgac cgccgagtcg
     2341 tattgttcga gccgcccgcg attgagcacg gcgacccggt cggccatgct catcgcctct
     2401 tcctgatcgt gcgtcaccat cacggtcgtg atattcagtt cgcgctggag acggcggatt
     2461 tcgatttgca tatcgagccg caggtttttg tcgagtgccg cgaaaggctc gtccagcagc
     2521 aggatgcgcg gctcgacggc aagcgtgcgg gccagtgcca cgcgttgttg ctgaccgccc
     2581 gacaactcgc gcggatagcg cttcgcgaat gcttccatgc gtaccatctc gagcatctgc
     2641 gtggccttgc gagccgcatc acgacgcgcg acgccgttgg cttccagacc gtaggcgacg
     2701 ttggcggcga ccgtcatgtg cggaaacagc gcatagttct gaaagacgat gcccacgccg
     2761 cgttgacgcg gcgcgaggcc gtccacgcgt tggccgccca cacggacttc accctcggac
     2821 tgcacctgca gaccggcgag gatgcgcaac agggtgctct tgccacagcc cgatgggccg
     2881 agcaatgcaa ccagctcgcc tggggcaatt tcaagcgtga cgtcttcgat caccggcacg
     2941 gcggcgaaac gatgccggat gccacgcatc tgtacggcgg cgccggtttg cgcggcgcgt
     3001 gcttgcgcgg ccgcaaggcc ggcgcgcgct gtgccgtgtt tagactgcga atcgatactg
     3061 tgcggggtgc tcacgcgctg gccctcttcc atagttgcgt ggcggcgtgc tttccagcgg
     3121 caatgacggc atcggcgtcg caacgggtgg gcgcgccgcc gtccaccacg atctccccgt
     3181 ccacgatgac catctcgaca tcacgcccaa gtcccgtgtg caccagcgtg cccaacggat
     3241 tggggtgcgg agtcagatgc gcgcgctgga aatcgaagat ggcgaggtcg gcgagccagc
     3301 ctgtcttgag gcagccgagt tcgtcgccaa gccccatggc gtgcgcgccg ttgtgcgtcg
     3361 ccatggcgag cgcatcggcg ggttgccagt ccgcagccac ttcgcccaac tggatgcgcg
     3421 cgatggccag cgcccagcgc atgacctcgg tcatatcggc gtgcatgttg tcggtgccca
     3481 atgcaatgcg cgcgccggcg gcgcgcagac gcggcgtagg cgccatcgtg ccgcccgtcg
     3541 cgttgccctt ggggatgtgc gccaccgtga tgccggcccg gcccacgcgt gcgatgtcgt
     3601 cgtcgtccag atacaggcag tgcgccgcta ccagccgttc gttgagcagc ccgacttcgt
     3661 ccagcaactg ggcggggctc ttgcccgaac gcttctttac gacgctgact tcggtgcggc
     3721 tttgcgcgag gtgagtcgtc acgcccagat catgcgtgcg tgccgcctcg gcgatcagac
     3781 gcagaaagtc gtcggagcag gtgtcaggcg catgaacggc tagttggacg cccgtgcgcc
     3841 cgttgggctt gcgattccag cggccggcca gcgtcagtgc gtcgttgagc gtgcgctcgc
     3901 cggtcgcggc atggtattcc cagcgcccgt ccgcgacgcc ggagaaatcg acgtcgtgca
     3961 ggcggccgca tgaatacact cgcaggccga ggtcggccat ggcctccatg gtcagatcgg
     4021 cgtggacgta ggtgtcgttg atcagcgtcg agccgaacag catggcttcg agcgcgccga
     4081 gacgcgccag cgcgatcgct tcgtccggac tcacgtcatg gccgtgggga atccctggcg
     4141 tgtaagcggg cgcgaagccc aggtcttcag cgacgccgcg caccattgtc agaatggcgt
     4201 gggtgtggac gttcacgtag ccgggcacga tgacgcgtcc ggcaagccgg cgcgtgctcc
     4261 gcgcgcgcat ctgtacgggc agatcctgcg cccggccgac aaaggcgagc cggttgccgc
     4321 gaatgccgat cgccgcgtcg tgcaacagcg gttgagccgg gtccatcgtc acgacagtgg
     4381 cgcccagcag caacaggtca aaatcaaatt cgataggtaa ggcagtcatc agccaggtgg
     4441 tccggttcag gtcaggtatg caggtgctgc aggaaacgcc gcgtgcgctc ttcgcgcgga
     4501 cggccgaaaa cgtcgccggg ggggccttgt tcgacgatgg cgccggcgtc catgaagacc
     4561 acccgatgcg cgacgttgcg cgcgaagctc atttcgtggg tcacgaccat catcgtcatc
     4621 cgggcggtgg cgagatcgcg catcacgtca agcacttcgt tgacaagttc cgggtcgagc
     4681 gccgaggtcg gttcgtcgaa caggatcatc tccggttcca tcgcgagcgc gcgggcaatc
     4741 gccacgcgtt gctgctggcc gccagagagt tgcgccggcc gtttgccgag gtgttgcgcg
     4801 agatggactt tttccaatgc caactgggcg cgagcgcggg ccgcatcgcg cgacagcccc
     4861 agcacgcgct gagggccggc ggcgacgtta tccagtgcac tcagatgcgc gaacaggttg
     4921 aagcgctgaa acacgaagcc gatgcggctg cgctgccgcg ccagttcggc ttcgggcagc
     4981 gtgcggcctt gctccgacag cccgatacgc tcgccgccaa ggcggatcgt gccgccgtcc
     5041 ggacgctcca ggaagttgag acagcgcagg agggtagtct tacccgaacc cgacggcccg
     5101 atcagcacga cggtctcgcc gtgttcgacc tgcagcgaga cggagggcag aatgaccttg
//
LOCUS       DATASH010000971         4158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1354036,
            whole genome shotgun sequence.
ACCESSION   DATASH010000971 DATASH010000000
VERSION     DATASH010000971.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1354036"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="VHV99_25940"
     CDS             <1..730
                     /locus_tag="VHV99_25940"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEX3638858.1"
                     /translation="SPTMTKLHEAIVVDLVDAIVSGVYPVGSSLPNEMELAVAHGVSR
                     TAAREAMQKLASLGLIQSRRRKGATVLARESWKLLDASVLDMAVLRVEDVSFFQALVE
                     ARLLIEPRAAELAAARASERDLAGIEASLNAMASEADGTRGADWAAADVAFHAGIIEA
                     AGNWVFTQLIGTVRAALDAGIRLTGKQALSAQASLEQHRAVFDAIRSRRPEEAHAAMT
                     RLLLATQRDFNALEKGAVRATLGR"
     gene            861..2573
                     /locus_tag="VHV99_25945"
     CDS             861..2573
                     /locus_tag="VHV99_25945"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012402777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HEX3638859.1"
                     /translation="MRKLRSQQWFGTHGKDGFIHRSWMKNQGIPHDNFDGRPVIGICN
                     TWSELTPCNAHFRELADYVKRGVREAGGLPLEFPVMSLGESNLRPTAMLFRNLASMDV
                     EESIRGNPLDGVILLVGCDKTTPALLMGAASCNLPALAVSGGPMLNGRYRGKPIGSGT
                     GVWQMSEEVRAGTMTQEEFTEAESCMNRSRGHCMTMGTASTMASMVEALGMGLPHNAA
                     IPAVDARRQVLAHMAGRRIVDMVHEDLTMDRILTREAFENAIRTNAAIGGSTNAVVHL
                     IALAKRIGVPLTLDDWELGSDVPCLVNLQPSGEYLMEDFYYAGGLPAVLKQLGEQGLL
                     HKNALTVNGKTIWDNVSSARNDDEKVITLFTAPFKPKAGIAVLRGNLAPNGAVIKPSA
                     ATQHLLKHRGRAVVFENVEELHAKVDDEALDIDEHCVMVLKGAGPKGYPGFAEVGNMP
                     LPKKVLQKGITDMVRISDGRMSGTAYGAVVLHVSPEAAAGGPLALVQTGDMIELDVEA
                     RRLHLDVSDEELERRRALWTKPASPLRGYVKLYVDHVLQADQGADLDFLVGSSGAPVP
                     RDSH"
     gene            2593..3513
                     /locus_tag="VHV99_25950"
     CDS             2593..3513
                     /locus_tag="VHV99_25950"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014251570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX3638860.1"
                     /translation="MHTTAKNDFSPAGIWPVLYAYFDEHNRLDRDAMRAQVERTIAAG
                     APGIVILGLATEVQRLSRAEREQVVNWASEDIASRVPLAVTVTGDTVREQTEFGNWAV
                     ARGAQWLILQPPSTRTEPERFYFDFFAAVMNGLTDTAGAGIQNAPEYLGVGLSPEALA
                     ELAQQCPNFRWLKGEGAATVLRTTIDCLAAAGSTMPVFNGRGGQELLDNLRAGCAGMI
                     VAPDSFDHQVEIYRAFAHDPAAAQLHYEEVLPAIVFVMQSLDALTCYGKRIAAWRMGF
                     EVRHDRGMAPTAFGVECARRFADSLGSYGG"
     gene            complement(3534..>4158)
                     /locus_tag="VHV99_25955"
     CDS             complement(3534..>4158)
                     /locus_tag="VHV99_25955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018435506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX3638861.1"
                     /translation="FVFIEDWYWATQRIPEVEASKTSADDMSCLVLASGPADHQETCS
                     LFFVEAINAARSRIWITTPYLIPDEAVFSALRLAVLRGVDVRILIPSRRDHRVVFAAS
                     KLYAYDALLAGVKIFRYRPGFLHQKVVLVDDMSAAIGSANLDNRSFRLNFEIMVLTVD
                     RGFASEVEKMLLDDFAQSWEISRDDYLGAPAWRRMAMHVARLFSPIL"
BASE COUNT          818 a         1211 c         1370 g          759 t
ORIGIN      
        1 ctcgccaacc atgacgaagc tgcatgaggc gatcgttgtc gatctagtcg acgcgatcgt
       61 ctcgggcgtc tacccggtcg gctcgtcctt gccgaacgaa atggaactcg ccgtcgcaca
      121 cggcgtaagc cgcacggcgg cgcgcgaggc gatgcagaag ctcgcttcac ttggcctgat
      181 ccagagcagg cgacgcaagg gcgcgaccgt tctcgcgcgt gaatcgtgga aattgctgga
      241 tgcgtcggtg ttggacatgg cggtcttgcg cgtagaggac gtgtcgtttt ttcaggcact
      301 ggtcgaagcg cgcctgctga tcgaaccacg tgccgccgaa cttgctgcag caagggcttc
      361 cgagcgtgat cttgcaggga tcgaagcttc gctgaacgcc atggccagcg aagcggacgg
      421 cacgcgcggc gcggactggg cggcggcgga tgtcgctttt cacgcgggca ttatcgaagc
      481 ggccgggaat tgggtcttca cgcaactgat cggcacggtc cgggccgcgc tcgacgcagg
      541 cattcgcctg accggtaagc aagcgctgtc ggcgcaggcg tcgctggaac agcatcgcgc
      601 tgtattcgat gcaatccgga gccgccggcc cgaggaggcg cacgcggcaa tgacgcgact
      661 gttgctcgcc acccaacgtg acttcaacgc gctggagaag ggcgcggttc gagcaacgct
      721 tggccggtag cgatcaggcg gccggcgacg ctgattcaca ggaacaagaa aaggacatcg
      781 ggtaatcgcg cgcggtccac gaaccggcat gcccatccct cactactcca cataacggcc
      841 ctccgcgagg agacaaagac atgcgcaaac ttcgctcgca acaatggttc ggtacacacg
      901 gcaaggacgg tttcattcac cgctcgtgga tgaaaaacca gggcattcca cacgacaatt
      961 tcgatggccg tccggtgatc ggcatctgca acacctggtc ggaactgacg ccgtgcaacg
     1021 cccatttccg cgagctggcc gactacgtga aacggggtgt tcgcgaggcg gggggactac
     1081 cgctcgaatt cccggtgatg tcactcggtg aatcgaactt gagaccgacc gccatgctgt
     1141 tccgcaatct ggcctccatg gatgtggagg aatccattcg cggcaatccg cttgatggcg
     1201 tgatcctgct cgtcggctgc gacaagacta cacccgcgtt gctgatgggc gcggcatcgt
     1261 gcaacttgcc cgcgctcgcc gtatccggcg gaccgatgtt gaacggccgg tatcgcggca
     1321 agccgatcgg ctcgggcacg ggcgtctggc agatgtcgga ggaagtacgc gcaggcacga
     1381 tgacgcaaga ggaattcacc gaagcggagt cgtgcatgaa ccgttcgcgc ggacactgca
     1441 tgacgatggg aacggcttcc accatggcgt cgatggtcga ggcgctcggc atgggcttgc
     1501 cgcataacgc ggccattccc gccgtcgatg cgcgccgtca ggtgctggca catatggcgg
     1561 gccgcaggat tgtcgatatg gtgcatgaag acctcaccat ggaccggatc ctcacgcgcg
     1621 aagccttcga aaacgcgatc cgcacgaatg ctgcgatcgg cggatcgacg aacgcggtgg
     1681 tgcatctgat cgcactcgcc aagcgcattg gcgtgccgtt gacgcttgac gattgggaac
     1741 tgggttcgga cgtaccgtgt ctcgtgaatc tccagccatc gggtgagtat ctgatggagg
     1801 atttctacta cgcgggcggc ttgccggccg tgctgaagca gcttggcgaa cagggtttgc
     1861 tgcacaaaaa cgcgctgacc gtgaacggca agacgatctg ggacaacgtg tccagtgccc
     1921 gcaacgacga cgaaaaagtc atcacgctgt tcaccgctcc gttcaagccg aaggcgggta
     1981 tcgccgtgct gaggggaaat cttgcgccaa atggcgccgt gatcaaaccg tcggcggcaa
     2041 cgcaacattt gctgaaacat cgcggccgcg cggtcgtgtt cgagaacgtg gaggaattgc
     2101 acgcgaaagt ggacgacgaa gcgcttgata tcgatgagca ctgcgtcatg gtgttgaagg
     2161 gcgcagggcc gaagggctat ccggggttcg ctgaagtcgg caacatgccg ctgccgaaaa
     2221 aggtgctgca gaagggtatt acagacatgg tgcgcatttc ggatggccgg atgagcggca
     2281 ctgcgtacgg cgcggtggtg ttgcacgtgt cgccggaagc ggcagcgggc ggcccgctcg
     2341 cgctcgtgca gaccggcgac atgatcgagc tcgatgtcga ggcgcgccgg ctgcatctgg
     2401 acgtatccga cgaagaactc gagcggcggc gtgcgttatg gaccaagccg gcgtcgccac
     2461 tgcgcggtta tgtgaagcta tacgtcgatc acgtgctgca ggcggatcag ggcgcagacc
     2521 tcgatttcct cgtcggctcg agcggcgcgc ctgtgccgcg cgactctcat tgaacattgg
     2581 agatcaggtc cgatgcatac gactgcaaag aacgattttt cgcctgccgg tatctggcca
     2641 gttctctacg cttatttcga tgaacacaac cggcttgatc gtgatgccat gcgggcgcag
     2701 gttgagcgga ccatcgccgc aggtgctccc ggtatcgtga tactcggtct tgcaaccgag
     2761 gtgcagcggc tgtcgcgcgc cgagcgtgag caagtggtga actgggcgag cgaggacatt
     2821 gcatctcgtg tcccgctggc ggttactgtc accggcgaca ccgtccggga gcagactgaa
     2881 ttcggcaatt gggccgtggc gcgcggtgcg caatggctga tcctgcaacc gccgtccacg
     2941 cgcaccgagc ccgaacgctt ttacttcgat ttcttcgccg ccgtcatgaa cgggttgacg
     3001 gacacggccg gcgcagggat tcaaaatgcg cccgagtacc tgggcgtcgg tttgtcgccg
     3061 gaagcgctcg ccgagctggc gcagcaatgc ccgaacttcc gctggttaaa gggtgagggc
     3121 gcggcaacgg tgctgcgtac gaccatcgac tgcctggcgg cggccggcag cacaatgccc
     3181 gtcttcaacg ggcgcggcgg ccaggagttg ctcgacaatc tgcgcgccgg ctgcgcgggc
     3241 atgatcgtcg cgccggacag cttcgatcat caggtggaaa tttatcgggc attcgcgcac
     3301 gatccggccg cggcgcagtt gcactatgag gaggtgttgc cggcgattgt cttcgtgatg
     3361 cagtcgctcg atgcgctcac gtgttacggc aagcgcatcg cggcgtggcg aatggggttt
     3421 gaggtcaggc acgatcgcgg gatggcgccg acagcattcg gtgtggaatg tgcacggcgc
     3481 ttcgccgata gtctcggatc ttacgggggt taaaagcgcg ggttaaaacc gcgtcaaaga
     3541 atcggtgaga acaagcgcgc gacgtgcatc gccatgcggc gccacgcagg ggcgccaaga
     3601 taatcgtcgc ggctgatttc ccatgattgc gcgaagtcat cgagcagcat tttctcgact
     3661 tcgctggcaa agccccggtc cacggtcagg accatgatct cgaagttgag gcgaaacgag
     3721 cggttgtcga gattcgcgct gccaatggcg gcggacatgt cgtccaccag caccactttc
     3781 tgatgcagga agcccgggcg atatcgaaaa atcttcacgc cggcgaggag tgcgtcgtag
     3841 gcatagagtt tcgatgcagc aaacaccacg cgatgatcgc gccggctcgg aatcaggata
     3901 cgcacgtcca cgccgcgcaa tacggcgaga cgcagcgccg agaacacggc ttcgtcgggg
     3961 atcagatacg gcgtcgtgat ccagatccgc gaacgagccg cgttgattgc ttcgacgaaa
     4021 aacagcgagc acgtttcctg atggtcggcg gggccgctcg cgaggacgag gcagctcatg
     4081 tcgtccgccg atgtcttcga cgcttccacc tccggaatgc gttgcgtcgc ccagtaccag
     4141 tcttctatga acacaaac
//
LOCUS       DATASH010000972         1878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6492857,
            whole genome shotgun sequence.
ACCESSION   DATASH010000972 DATASH010000000
VERSION     DATASH010000972.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6492857"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /gene="xseA"
                     /locus_tag="VHV99_25960"
     CDS             <1..859
                     /gene="xseA"
                     /locus_tag="VHV99_25960"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEX3638862.1"
                     /translation="AAAQQKIERAIASGDALAKLAAMVEAASARREIDVLIVCRGGGS
                     IEDLWAFNEEVLARAIAESAIPVVSGVGHETDFTIADFAADVRAPTPTGAAELVSPQR
                     VLLLRELDHRHATLARGFGRMMERRAQQLDWLARRLVSPAERLTRQRTHLQQLSVRLA
                     SAGAGPVRDARARFSLLQMRWQRWRPDLAAYQARLGNLAQRLDAALLRQHERQVARID
                     TLAARLEVLSPQRTLERGYAAMIDARSGRAVRAPSSLKPGRRLTVHLAEGSADIALAD
                     VQERLADGL"
     gene            1079..1657
                     /gene="sodB"
                     /locus_tag="VHV99_25965"
     CDS             1079..1657
                     /gene="sodB"
                     /locus_tag="VHV99_25965"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006024786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HEX3638863.1"
                     /translation="MEHTLPPLPFARNALAPHMSEETLEFHYGKHHQTYVTNLNNLIK
                     GTEFESLSLEEIVKKSSGGVFNNAAQVWNHTFFWNSLSPQGGGAPTGALADAINAKWG
                     SFEKFKEEFAKTAVGTFGSGWAWLVKKADGSLDLVSTSNAATPLTTDAKALLTIDVWE
                     HAYYIDYRNARPKFVEAYWNIVNWEFAAKNFA"
BASE COUNT          354 a          563 c          622 g          339 t
ORIGIN      
        1 tgctgcagcg cagcaaaaga tcgagcgcgc gatcgcgtcg ggcgacgcgc tcgccaagct
       61 ggcggcgatg gtcgaggcgg ccagtgcgcg gcgcgaaatc gacgtgctga tcgtgtgccg
      121 cggcggcggt tcgatcgaag acctgtgggc cttcaacgaa gaggtgttgg cgcgcgcgat
      181 tgcggaaagc gcgatcccgg tggtgagcgg cgtcggtcac gaaaccgatt tcacgatcgc
      241 tgatttcgcc gccgacgtgc gcgcgccgac gccgaccggc gccgccgaac tcgtcagccc
      301 gcaacgcgtg ctgctcctgc gcgaactcga tcatcggcac gcgacgctcg cacgcggctt
      361 tggccgcatg atggagcggc gcgcgcagca actcgactgg ctcgcgcgcc ggctcgtgtc
      421 gccggccgag cgtctgacgc ggcaacgcac gcatctgcag caattgagcg tgcggcttgc
      481 gtcggcgggt gcgggtccag tgcgtgacgc gcgggcgcgc ttttcgttgt tgcagatgcg
      541 ctggcagcgc tggcgtccgg atctggctgc ttatcaagcg aggctcggca atctcgcgca
      601 acgtctggac gcggctctgt tgcgccagca tgaacgacag gtcgcgcgca tcgatacgct
      661 tgccgctcgg ctcgaggtgc tgagtccgca acgcaccctg gagcggggct atgcggccat
      721 gatcgatgcg cgcagcggcc gcgcggtgcg cgcgccgtca tcgttgaaac cggggcggcg
      781 cctgacggtg cacctcgccg aaggttcggc cgacatcgca ctggccgatg tgcaggagcg
      841 tctcgccgac ggtttgtgac acttcgccga cggccgcggc agtcatgcgc gtgactgccg
      901 gatgaggcgg gcacacgccg tgcgaatgcg atgattacac tctatgcgcg gcgtgttttc
      961 cgctgccggg cataaagtcc gttggtttgc ggggttattc caagtcgcct acaatcgagt
     1021 gctctaaagc gttatccgca gactccccca caacagatac agacagaagg aaagcaccat
     1081 ggaacatacg ctcccgccgc tgccgttcgc gagaaacgca ctcgctccgc acatgtcgga
     1141 agagacgctc gagtttcact acggcaagca tcaccagacc tatgtgacca acctcaataa
     1201 tctgatcaag ggtacggagt tcgagagcct gtcgctggaa gagatcgtca agaagtcgtc
     1261 gggcggcgtg ttcaacaacg cggctcaagt gtggaaccac acgttcttct ggaacagcct
     1321 gtcgccgcaa ggtggcggcg caccgaccgg cgcgctggct gatgcaatca acgccaagtg
     1381 gggctcgttc gagaagttca aggaagaatt cgcgaagacc gcggtcggca ctttcggctc
     1441 cggctgggca tggctcgtga agaaggccga cggttcgctc gacctcgtgt cgacgagcaa
     1501 cgccgctacg ccgctgacca ccgatgcaaa agcactgctg acgatcgacg tgtgggaaca
     1561 cgcgtactac atcgactacc gcaatgcgcg tccgaagttc gtggaagcgt actggaatat
     1621 cgtcaattgg gaatttgctg cgaagaactt cgcgtgagat tgctggctat cgcatgacgg
     1681 gctaattggc atcgcctgat caatcgcgcg atttcgcatt gcctaccgct atggcggtcg
     1741 gcagcggtag tgaaaagtaa aagccctcct gagtgagggc tttttatttg tgcgccgagc
     1801 atgcgcaaca gcttgggggt gcaagtcccc tgtccagcct gatggggcga aggactagcg
     1861 aaacgcaagg gcgtcgtc
//
LOCUS       DATASH010000973         5573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2539459,
            whole genome shotgun sequence.
ACCESSION   DATASH010000973 DATASH010000000
VERSION     DATASH010000973.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5573
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2539459"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VHV99_25970"
     CDS             complement(<1..518)
                     /locus_tag="VHV99_25970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018444185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX3638864.1"
                     /translation="MSGTLLLSEQLIARASVTPDDQHCQNLLIERLMAIGFECETIAS
                     GGVTNLWAVKRGTDGTDGKLLAFAGHTDVVPTGPLEQWTSPPFAPTHRDGKLYGRGAA
                     DMKTSIAAFVVSSEEFIAAHPAHRGSLAFLITSDEEGPATDGTVKVIEALKARGERLD
                     YCIVGEPTSSATL"
     gene            complement(633..995)
                     /locus_tag="VHV99_25975"
     CDS             complement(633..995)
                     /locus_tag="VHV99_25975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HEX3638865.1"
                     /translation="MAAKKIVVYGIPNCDTVRKARVWLEEHDVPFEFHDFKKAGVSTA
                     LLQDWLKDVTLDELINRRGTTWRGLSDTYKDEAGSTAGAIALMIHKPSIIKRPVLVVN
                     GRVKSLGFDAEKYETLFV"
     gene            complement(999..1826)
                     /gene="dapD"
                     /locus_tag="VHV99_25980"
     CDS             complement(999..1826)
                     /gene="dapD"
                     /locus_tag="VHV99_25980"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007580558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEX3638866.1"
                     /translation="MSQQLQQIIDTAWDNRAELSPKSAPAEVRDAVAHAIEQLDKGLA
                     RVAEKRDGEWIVNQWLKKAVLLSFRLEDNAPMPAGGYSQFYDKVPSKFANYTAEDFAA
                     GGFRVVPPAIARRGAFIAKNVVLMPSYTNIGAYVDEGTMVDTWATVGSCAQIGKNVHL
                     SGGVGIGGVLEPLQANPVIIEDNCFIGARSEVVEGVIVEENSVISMGVYLGQSTKIYD
                     RETGSVTYGRIPAGSVVVAGNLPSKDGSHSLYCAVIVKKVDAKTRAKVGLNELLRGD"
     gene            complement(1865..3091)
                     /gene="dapC"
                     /locus_tag="VHV99_25985"
     CDS             complement(1865..3091)
                     /gene="dapC"
                     /locus_tag="VHV99_25985"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006498676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HEX3638867.1"
                     /translation="MNPLLQKLQPYPFEKLRALFKRVTPCADLAPISFGIGEPKHATP
                     ALIREAVVNAMSGLASYPATAGSEPLRQAIADWTKERYGLPAVNAATEVLPVSGSREA
                     LFSLAQTVIDSRSAGEPPVVVCPNPFYQIYEGAALLAGAEPYFVNSNPARNFACDYAA
                     VPDAVWARTQLLYVCSPGNPTGAVLTLEDWRELFALSDRHGFVIASDECYSEIYFNEA
                     APPLGCLEAAHKLGRGFDRLVMLSSLSKRSNVPGMRSGFVAGDASILKQFLLYRTYHG
                     AALSPMFQAASVVAWKDEAHVRENRALYLKKFTTVTPMLAEVLDVRLPDAAFYLWANV
                     ARTGLTDTEFAARLYADYNVTVLPGSYLARDAHGTNPGSGFIRVALVADVDECVEGAR
                     RIVEFCSTLAQHRLPQ"
     gene            3637..>5573
                     /gene="smc"
                     /locus_tag="VHV99_25990"
     CDS             3637..>5573
                     /gene="smc"
                     /locus_tag="VHV99_25990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013338875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEX3638868.1"
                     /translation="MRLTSIKLAGFKSFVDPTHFQVPGQLVGVVGPNGCGKSNIIDAV
                     RWVLGESRASELRGESMQDVIFNGSTARKPGSRASVELIFDNADGRAGGQWGTYAEIA
                     VKRVLTRDGTSSYYINNLPARRRDIQDIFLGTGLGPRAYAIIGQGMIARIIEAKPEEL
                     RVFLEEAAGVSKYKERRRETENRLHDTRENLTRVEDIVRELSANLEKLEAQAIVATKY
                     KALQADGEEKQRLLWLLRKNEAGNESERQQRAISDAQIELEAQTAKLREVEAQLETLR
                     VAHYSASDAMQGAQGSLYEANSEVAKLEAEIRFIVESRNRVQAQIAALSAQREQWQMQ
                     AEKARGEITDAEEALAVGEEKAAIAQETATAKHDELPALEARFRDAQAELNAERGGIA
                     QTEQALKLEAAHQRNADQQLQQLQQRHERLKSEAGGLDAPDEAQLEELRMQLAEHEEI
                     LAEAQARLSDAQDTLPRLDAERRAAQEKVQAESAQIHQLEARLAALKQLQENVQTEGK
                     IQPWLDKHELGSLPRLWKKLHVEAGWETALESVLRERIAALEVSNLDWVKAFATDAPP
                     AKLAFYAPPNACRPQETPAGLRPLLALIRIDDAGLRAVLTDWLGQIFVADDIAQALAS
                     RSQLPDGAAFVVKAGHVVTRVG"
BASE COUNT         1116 a         1700 c         1756 g         1001 t
ORIGIN      
        1 agcgtcgcgc tggaagtggg ctctccgaca atgcagtaat ccagccgctc gccacgcgct
       61 ttcaaagctt cgatcacttt gacggtgccg tcggtggcgg ggccttcttc atcgcttgta
      121 atcaggaagg cgagcgagcc gcgatgcgca gggtgcgccg cgatgaactc ttcgctcgac
      181 acaacgaacg ctgctatgga cgtcttcatg tccgctgcgc cgcggccgta cagcttgccg
      241 tcgcggtggg tcggcgcgaa gggtggcgac gtccattgtt ccagcggacc ggtcggtacc
      301 acgtcggtgt ggccggcaaa cgctagcaac ttgccgtcag tcccgtccgt gccacgtttg
      361 acggcccaga gattcgtgac gccgccggac gcgatcgttt cgcactcaaa gccaatcgcc
      421 atcaaccgct cgatcagcag gttctggcag tgctggtcgt cgggcgtgac ggacgcgcgc
      481 gcgatgagtt gttccgagag aagaagggtg ccggacatgg attcgagcca ctttcaataa
      541 aaatgccggc gcggcggccg gcagggatca agattcgcgc gccttttatc gggggcacgc
      601 gatgctgcgc ttaaaagctt gaggctcaat tcttaaacga acagcgtttc gtacttctcg
      661 gcatcgaaac cgagcgattt cacgcggccg ttcacgacga gcacgggccg cttgatgatc
      721 gacggcttgt ggatcatcaa cgcgatcgcg cctgcagtgg aaccggcttc gtccttgtag
      781 gtgtccgaca ggccgcgcca ggtcgtgcca cgccggttga tcaattcgtc gagcgtgacg
      841 tccttcaacc agtcctgcaa cagggccgtc gaaacgccgg ctttcttgaa gtcgtggaat
      901 tcgaacggca cgtcatgttc ttccagccac acgcgcgctt ttctcacggt gtcgcaattc
      961 ggaattccat acacgacgat ctttttcgcg gccatggatc aatcgcctcg cagcaactcg
     1021 ttcaggccga ccttggcgcg tgtttttgcg tccaccttct tcacgatcac cgcgcagtac
     1081 aagctgtgcg aaccgtcctt cgaaggcagg ttgcccgcga ccaccaccga gcccgccgga
     1141 atgcggccat acgtgaccga acccgtttcg cgatcataaa tcttggtgct ctggccgaga
     1201 tacacgccca tggagatgac tgagttctct tccacgatca cgccttccac cacttccgaa
     1261 cgtgcgccga taaagcagtt gtcttcgatg atcaccgggt ttgcctgcag cggttccagc
     1321 acgccgccaa tgcccacgcc acccgacaag tgcacgttct tgccgatctg tgcgcacgaa
     1381 ccgaccgtcg cccaggtatc gaccatcgtg ccttcatcca catatgcgcc aatgttcgta
     1441 tacgacggca tcaatacaac gttcttcgcg atgaacgcac cgcggcgagc aatggccggc
     1501 ggcacgacgc gaaagccgcc cgcggcgaaa tcttcagccg tgtagttggc gaacttcgac
     1561 ggcactttgt cgtagaactg gctgtatccg ccagccggca tcggtgcatt gtcttccagg
     1621 cggaacgaca gcagcaccgc cttcttcagc cattgattca cgatccattc gccgtcccgt
     1681 ttctcggcaa cgcgtgccaa gcctttatcg agttgctcga tggcgtgagc gacagcgtcg
     1741 cgcacctcgg cgggggccga tttcggcgac agctcggcgc ggttgtccca ggcggtatcg
     1801 atgatctgct gaagttgttg cgacatggtc gtgacttaag tgagtgctgc gatttaagga
     1861 ggggttactg cggcagccga tgctgagcca gcgtgctgca aaattcgacg atacgccgtg
     1921 caccttccac acattcgtcg acgtcggcga ccagcgcaac gcgaatgaag ccgctgccgg
     1981 gattcgtgcc gtgggcgtcg cgtgccagat acgagcccgg caaaaccgtc acattatagt
     2041 cggcgtacag gcgggcggcg aactcggtgt cggtgaggcc ggtgcgcgcc acgttcgccc
     2101 acagatagaa cgcggcgtcg ggcagacgga cgtcgagcac ctcggcgagc atgggggtga
     2161 cggtcgtaaa tttcttgagg tacagcgcgc ggttctcgcg cacatgcgct tcgtctttcc
     2221 acgcaacgac gctcgctgcc tggaacatgg gagagagcgc tgcgccgtga taggtgcggt
     2281 agagcaggaa ttgcttgagt atcgacgcgt cgccggcgac aaaacccgag cgcatgccgg
     2341 gcacgttcga gcgcttggac aagctcgata acatcaccag ccggtcgaaa ccgcggccga
     2401 gcttgtgtgc ggcttccagg cagccgagcg gaggcgcggc ttcgttgaaa tagatctcgg
     2461 aataacattc atcggaggca atcacgaagc cgtggcgatc cgagagcgcg aatagctcgc
     2521 gccagtcttc cagcgtcagc acggcgccgg tcgggttgcc cggcgagcag acgtagagca
     2581 attgggtgcg cgcccagacg gcatcgggga cggctgcgta atcgcaggcg aagttgcgcg
     2641 ccgggttgct gttcacgaag tacggctcgg cgccggccag cagcgctgcg ccttcataga
     2701 tctggtagaa cggattcggg cagacgacaa cgggtggttc acccgcgctg cggctgtcga
     2761 tcactgtctg ggcgagcgaa aaaagcgctt cgcgcgagcc ggaaacgggc aaaacttccg
     2821 tggcggcatt gaccgcgggc aggccgtagc gttctttcgt ccagtcagcg atggcctggc
     2881 gcaacggctc gctgcccgca gtggcgggat aggacgcgag gccggacatc gcgttcacga
     2941 ctgcctcgcg gatcagcgcc ggcgttgcat gtttcggctc gccgataccg aagctgatgg
     3001 gcgccaaatc cgcgcacggc gtaacgcgtt tgaatagcgc gcgcaacttt tcgaatggat
     3061 acggctgaag tttctggaga agtggattca cggcgaagtc ggtgcagacg ttgaaacgac
     3121 aaggctcgga ttatatcccc ctcgtcgctc gccgtagctc cctggttggg tttgccgggg
     3181 ctttgagacg gctctgccgg cgcgcggaac gcggatttcc gcagtggaca tgcgctcgac
     3241 catggccgcg gccagccaag ccgaaatcgc ccggcatcga ccgtgcggaa cccgggccgg
     3301 cctgccgttg cgaggagaaa tccctgtgtc agcaaaccaa aagaattcaa gacgtgcgat
     3361 ggtatgattg tcaccgttcg ccgggttgcg tgcttgcggt cggttacagg ttgtatataa
     3421 gagttatata tataagccgt cgagcaatag caccacgggt tgcgctccgc cgcttcgaat
     3481 cgagctttga tcgaatcgag ctggattcaa cggggttcga cccaccgccc gagcgttttc
     3541 gaccggcgtt agcagttatc tcctgccgca caagcatcac ggctcggagg ctcgccggcc
     3601 cgttctcccg aattcttttc aatcagcgat atcgccgtgc gtctgacctc gataaaactc
     3661 gctggcttca agtccttcgt cgatcccacc catttccagg tcccaggcca gcttgttggc
     3721 gtggtggggc cgaatggctg cggcaaatcc aacatcatcg acgccgtgcg ctgggtgctc
     3781 ggcgaatcgc gcgcttccga gctgcgcggc gagtcgatgc aggacgtgat cttcaacggc
     3841 tccacggcgc gcaagccggg cagccgcgcc agcgtcgaac tcatcttcga taacgccgac
     3901 ggccgggccg gtggccagtg gggcacctac gccgaaatcg ccgtcaagcg cgtcctcacg
     3961 cgcgacggca cgtcgagcta ttacatcaac aacctgccag cccgccgccg cgatatccag
     4021 gacatcttcc tcggtacagg cctcggaccg cgcgcctacg cgatcatcgg gcagggcatg
     4081 atcgcgcgga tcatcgaagc gaagcccgaa gaactgcgcg tgttcctgga agaagccgca
     4141 ggcgtgtcca agtacaagga acgccgccgc gagaccgaaa accgcctgca cgatacccgc
     4201 gaaaacctga cccgcgtgga agacatcgtg cgcgagttga gcgcgaatct ggagaagctc
     4261 gaagcccagg cgattgtcgc gacgaagtac aaggcgctgc aggccgacgg cgaggaaaag
     4321 caacggcttc tgtggttgtt gcgcaaaaac gaggcgggca acgagtcgga gcgccagcag
     4381 cgcgcgatca gcgacgcgca gatcgagctc gaagcgcaaa ccgccaagct ccgtgaagtc
     4441 gaagcgcaac tggaaacgct gcgggtggcg cattacagcg caagcgacgc catgcagggc
     4501 gcccagggct cgctgtacga agcgaactcg gaagtggcca agctcgaggc ggagatccgc
     4561 tttatcgtgg aatcgcgtaa tcgggtgcag gcgcagatcg ctgcgttgag cgcccagcgc
     4621 gagcagtggc aaatgcaggc cgagaaagcc cgtggcgaaa tcacggatgc cgaagaggcg
     4681 ttggctgtcg gcgaagaaaa ggccgcgatc gcgcaggaaa cagctaccgc aaaacacgac
     4741 gaattgcccg cgctcgaagc acgttttcgc gacgcccagg ccgagttgaa cgcagagcgc
     4801 ggcggtatcg cgcagaccga acaagcgttg aaactcgagg ccgcgcatca gcgcaacgcc
     4861 gaccagcaac tgcagcagtt gcagcagcgt cacgaacgcc tgaaaagcga agcgggcggc
     4921 ctggacgcgc ctgacgaagc gcagctcgaa gagttgcgca tgcaactcgc tgagcacgaa
     4981 gaaattctcg ctgaagctca agcccggctc tccgatgcac aggacacgct gccgcgtctg
     5041 gatgccgagc gtcgcgcggc acaagagaaa gtgcaggccg aaagcgcgca gattcaccag
     5101 ctcgaagcac ggctcgcggc gttaaaacag ttgcaggaaa acgtccagac ggaaggcaag
     5161 atccagccgt ggctcgacaa acacgagctc ggttcattgc cgcgcctgtg gaaaaagctg
     5221 cacgttgaag caggttggga aacggcgctg gagtcggttc tgcgtgagcg gatcgcggcg
     5281 ctggaagtat cgaatctcga ctgggtgaag gcgttcgcga ccgacgcgcc acccgcgaag
     5341 ctcgcgttct acgcacctcc caacgcctgc cgtccgcagg aaacgcccgc cggcttgcgg
     5401 ccgctgcttg cgctgatccg tatcgatgac gcaggtctgc gcgccgtgct gaccgattgg
     5461 ctgggccaga tttttgtcgc cgatgacatc gcccaggcgc tcgcctcgcg ttcgcaattg
     5521 ccagacggcg ctgcgttcgt ggtgaaggcc gggcatgtgg tgacgcgcgt cgg
//
LOCUS       DATASH010000974         6049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4320101,
            whole genome shotgun sequence.
ACCESSION   DATASH010000974 DATASH010000000
VERSION     DATASH010000974.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6049
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4320101"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="VHV99_25995"
     CDS             <1..457
                     /locus_tag="VHV99_25995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HEX3638869.1"
                     /translation="ETMAAICLEAEKSEQSELDRDFLDRTFTRIDQSIAMGALFTAFH
                     LGAKAIVALTESGSTALWMSRHWTHVPIFALTPRVGSERAMAIYRNVTSLHLDTSTDR
                     DTALQQALETVVGKGYASRGDMVVLTVGEPMGQAGGTNTLKIVRVGEPY"
     gene            887..1951
                     /gene="fba"
                     /locus_tag="VHV99_26000"
     CDS             887..1951
                     /gene="fba"
                     /locus_tag="VHV99_26000"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589515.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEX3638870.1"
                     /translation="MPLVSMRQLLDHAAENGYGIPAFNVNNLEQVQAIMAAADKVNAP
                     VIMQASAGARKYAGEPFLRHLIEAAVESYPHIPVVMHQDHGQSPAVCMAAIRSGFTSV
                     MMDGSLEADGKTVASYEYNVEVSRKVVEAAHSIGITVEAELGVLGSRETMKGDKEDGH
                     GAEGTMTREQLLTDPEQAADFVKLTQCDALAIAIGTSHGAYKFSKKPTGDILSIQRIK
                     EIHQRIPNTHLVMHGSSSVPQELLAEIREFGGDMKETYGVPVEEIQEGIKNGVRKVNI
                     DTDLRLAITGAIRRYMATNPGKFDPRDYLKPAREAAMKICLERFTQFGCEGQAGKIKP
                     VSLDKIAEKYKSGELAQLVR"
     gene            2084..2974
                     /locus_tag="VHV99_26005"
     CDS             2084..2974
                     /locus_tag="VHV99_26005"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX3638871.1"
                     /translation="MSTLYESTLHSLPLLGRGKVRDNYAVGNDQLLIVTTDRLSAFDV
                     IMGEPIPNKGRVLNEMANFWFEKLKHVVPNHLTGVAPESVVAADEVEQVKGRAVVVKR
                     LEPILVEAVVRGYLAGSGWKDYQATGSVCGVELPPGLQNAQKLPEPIFTPAAKAEMGH
                     HDENITYNEMERRIGTELSATIRDISIRLYKEAADYAATRGIIIADTKFEFGLDNHGQ
                     LYLMDEALTADSSRFWPADQYQVGTNPPSFDKQFVRDWLETQPWKKEPPAPKLPDDVV
                     TKTGEKYQEALERLTGHKLA"
     gene            3123..3641
                     /gene="purE"
                     /locus_tag="VHV99_26010"
     CDS             3123..3641
                     /gene="purE"
                     /locus_tag="VHV99_26010"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007581425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX3638872.1"
                     /translation="MSEAQTAHTHGAPVVGVLMGSSSDWEVMKNAVAILQEFGVPYEA
                     KVVSAHRMPDEMFVYAERARERGIRAIIAGAGGAAHLPGMLAAKTTVPVLGVPVASKY
                     LKGVDSLHSIVQMPKGVPVATFAIGEAGAANAALFAVSILSSTSAEYAEKLAAFRVRQ
                     NEAAHAMVLPAL"
     gene            3746..4942
                     /locus_tag="VHV99_26015"
     CDS             3746..4942
                     /locus_tag="VHV99_26015"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEX3638873.1"
                     /translation="MNPDNTPVSPILPGAWLGMVGGGQLGRMFCFAAQAMGYRVAVLD
                     PDETSPAGAVADRHIRAAYDDEASLTELARLCAAVSTEFENVPAASLDFLARTTFVSP
                     AGRCVAVAQDRIAEKRFIASSGVAVAPHVVIESSDALAALDDAKLEAVLPGILKTARM
                     GYDGKGQIRVRNAEEVREAHASLAGVPCVLEKRLPLKFEVSALIARAASGASVVYPLA
                     QNTHRDGVLSHTIVPAPDASSTLVGQAQQAALQIADKLGYVGVLCVEFFILEDGSLVA
                     NEMAPRPHNSGHYTVDACATSQFEQQVRAMTGMPLGDTRQHSPAVMLNILGDVWFPDG
                     PKRAAVTPPWHEVAAMPAARLHLYGKEEARCGRKMGHVNFTAATLEEARTAGRDCARL
                     LHIITS"
     gene            4953..6005
                     /locus_tag="VHV99_26020"
     CDS             4953..6005
                     /locus_tag="VHV99_26020"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX3638874.1"
                     /translation="MPDQSKPAEGGAANALPVSAEQIGHAAALLYAGGLVAFPTETVY
                     GLGGDAESPDAVARIYAAKGRPANHPVIVHLAPQGDPNYWVEHLPAEAQRLIDAFWPG
                     PLTLILKRAARIDAAVSGGQDSVGLRCPSHPVAQALLEAFSALRGGHGGVAAPSANRF
                     GHVSPTTAQHVREEFGGAIHVLDGGASDVGIESTILDLSRGFPALLRPGRVTPQDIAD
                     VLGEAPRLPDGSDATAPRASGTLKAHYAPRTPLALLPFDALQSLLAARQGDERVALVA
                     RASRAGEWAHAEGVHFIAAPEDPHVYARELYGLLRALDRADVSRILIEKLPDTIEWIA
                     VNDRLGRAAAAFEAQG"
BASE COUNT         1121 a         1840 c         2041 g         1047 t
ORIGIN      
        1 cgagacgatg gcggcaatct gtctcgaagc cgagaaatcg gagcagtcgg agctggaccg
       61 cgatttcctc gaccgcacgt tcacgcggat cgaccagtcg atcgcgatgg gcgcgctgtt
      121 cacggcgttc catctcggcg cgaaggcgat cgtggcgttg accgaatccg gctcgaccgc
      181 gctgtggatg tcgcgtcatt ggacccatgt gccgattttc gcgctcacgc cgcgcgtcgg
      241 cagcgaacgg gccatggcga tctaccgcaa cgtcacgtcg ctgcatctgg acaccagcac
      301 tgaccgcgac accgcgttgc agcaggcgct ggagaccgtg gtcggcaagg gctacgcgtc
      361 gcgtggcgac atggtggtgc tgaccgtcgg cgagccgatg ggccaggcgg gcggcacgaa
      421 tacgctgaag atcgtgcggg tcggcgagcc ttattgatct ggtagcaacg atgttgcatg
      481 cgatcggcgc gctctgcgtg ggagtgctcg atcggcgcgc tctgcgtggg gccagagtga
      541 gtgctcaggc gctagctctg ggtgaccgcg ttgcatggga ccggagtagg cgctatggga
      601 ccggagtagg cgccaaagcg ctaactcggg tcgacagcgt tgcatgggat ccgagtaagc
      661 gctaaagcgc taactctggt cgacacgcca actcgggtcg acacacgctt gattgagcga
      721 agcaggctgc ccataccccg cgagaggcgg cgcacagact ccaatcgcgc gtgaaggaaa
      781 acgcgcttta gggaatgggt gaaagggtat gcagcttgct tcgcgataaa atcgcccaat
      841 aagatgccga cggcacagta ttcgtttcaa tctaaggagt tacagcatgc ctctcgtatc
      901 aatgcgtcaa ttgctggacc atgccgccga aaacggttac ggcattccgg cattcaacgt
      961 aaacaacctg gagcaggtgc aggcgatcat ggcggcggcc gacaaggtca acgccccggt
     1021 catcatgcag gcgtcggccg gcgcgcgtaa gtacgcgggc gagccgttcc tgcgccatct
     1081 gatcgaagct gcggtcgagt cgtacccgca tattccggtc gtgatgcacc aggaccacgg
     1141 ccagtcgccg gcggtctgca tggcggcgat ccgcagcggt ttcaccagcg tgatgatgga
     1201 cggttcgctc gaagccgacg gcaagacggt cgcctcgtac gagtacaacg tcgaagtctc
     1261 gcgcaaggtg gttgaagcgg cgcactcgat cggcatcacg gtggaagccg aactgggcgt
     1321 gctcggttcg cgggaaacca tgaagggcga caaggaagac ggccacggcg cggaaggcac
     1381 gatgacccgc gagcagttgc tgaccgaccc ggagcaggca gccgacttcg tcaagctgac
     1441 gcaatgcgac gccttggcga tcgcgatcgg tacgtcgcac ggcgcgtaca agttcagcaa
     1501 gaagcccacg ggcgacatcc tgtcgatcca gcgcatcaag gaaattcacc agcgcattcc
     1561 gaacacgcac ctggtgatgc acggttcgtc gtcggtgccg caggagctgc tcgcggaaat
     1621 ccgcgaattc ggcggcgaca tgaaggaaac ctacggcgtg ccggtcgagg aaattcagga
     1681 aggtatcaag aacggcgtgc gcaaggtcaa tatcgacacc gacctgcgtc tggcgatcac
     1741 cggcgcgatc cgccgctaca tggcgaccaa tccgggcaag ttcgatccgc gcgactacct
     1801 gaagccggcg cgcgaagccg cgatgaagat ctgtctcgag cgcttcacgc aattcggctg
     1861 cgaaggccag gctggcaaga tcaagccggt ttcgcttgat aaaatcgcgg agaaatacaa
     1921 gtcgggcgag ctcgcacagc ttgtccgcta agctataact aagcgtcagg cttggccgat
     1981 tggccaggtt gccgttagat cgccgttccc gcatcatccg gggaacggcg tttccctttt
     2041 gttccggtca cactttttgc cccactttta gcgaaccacg acgatgtcta ccctttacga
     2101 atccacgctc cactcgctgc cgctgctcgg ccgcggtaaa gtccgcgaca actacgcggt
     2161 gggcaacgac cagttgctga tcgtcacgac cgaccgtctg tcggcgttcg acgtcatcat
     2221 gggcgagccg attccgaaca agggccgcgt gctcaacgaa atggcgaact tctggttcga
     2281 gaaactgaag catgtggtgc cgaatcacct cacgggcgtg gcgcccgaat ccgtggtggc
     2341 ggcagacgaa gtcgagcagg tgaagggccg tgcggtcgtg gtcaagcgcc tcgaaccgat
     2401 cctcgtcgaa gcggtggtgc gcggctatct ggccggcagc ggctggaaag actatcaggc
     2461 aaccggctcg gtgtgcggcg tggaactgcc gcccggcctg caaaacgcgc agaagctgcc
     2521 cgagccgatt ttcacgccgg cggccaaagc cgaaatgggc caccacgacg aaaacatcac
     2581 gtacaacgag atggagcgcc gcatcggcac cgaactgtcg gccacgatcc gcgacatctc
     2641 gatcaggctg tacaaggaag ccgctgacta tgcggccacg cgcggcatca tcatcgcgga
     2701 cacgaaattc gagttcggcc tggacaacca cggccaactg tatttgatgg acgaggcgtt
     2761 gaccgccgat tcgtcgcgtt tctggccggc tgaccagtat caggtcggca cgaacccgcc
     2821 gtcgttcgac aagcagttcg tgcgtgactg gctcgaaacc cagccgtgga agaaagagcc
     2881 gcccgcaccg aagctgccgg acgacgtggt cacgaagacg ggcgagaagt accaggaagc
     2941 cctcgaacgc ctgaccggac acaagctcgc ctgagcacgc acgcctggta gatccgcaac
     3001 gtgcgaaaaa acgccgggcc gtcccaggtt tttcgccccg cagattttcg ccgcgcaggg
     3061 agtcctgccg gtgatcaccc ctcgggggct ggcgcgctct caagaaacaa ggaagccgta
     3121 agatgagtga agcacaaact gcccatacgc atggcgcgcc cgttgtcggc gtgctgatgg
     3181 gctccagctc cgactgggaa gtgatgaaga acgccgtggc gattctgcag gaattcggcg
     3241 tgccgtacga agcgaaggtc gtgtcggcgc accgcatgcc cgacgagatg tttgtctatg
     3301 ccgagcgcgc gcgcgagcgc ggcattcgcg cgatcatcgc gggtgcgggc ggcgcggcac
     3361 atctgccggg catgctggcc gccaagacca cggtgcccgt gctcggcgtg ccggtggcga
     3421 gcaagtatct gaagggcgtg gattcgctgc attcgatcgt gcagatgccc aagggcgtac
     3481 cggtcgccac gtttgccatc ggcgaagcag gcgcggcgaa tgcggcgctg ttcgcggtgt
     3541 cgattctgag cagcacgagc gccgagtatg cggaaaaact ggcggcgttc cgcgtgcgcc
     3601 agaacgaagc ggctcacgca atggtgctgc cggcgctgta agcgccaagg ccgattgctt
     3661 ttagacgtcc cccaatgtct gacccccggc cggatgccgc tattgcacgt tttgcatgca
     3721 catccggccg cgaccgacca ctaagatgaa cccagacaac acaccggttt caccgattct
     3781 gcccggcgca tggcttggca tggtcggtgg cggccagctc ggccgcatgt tctgttttgc
     3841 cgcgcaggcg atgggctatc gcgtcgctgt gctcgacccg gacgaaacca gtccggcggg
     3901 cgccgtggcc gaccgccata tccgtgcggc ttacgacgac gaagcgtcgc tgaccgaact
     3961 cgcgcgcctg tgcgcggcgg tctcgaccga attcgagaac gtgccggccg cgagcctcga
     4021 tttcctcgcc aggaccacgt tcgtcagccc ggcgggccgt tgcgtcgccg tggcgcaaga
     4081 ccggatcgcc gagaagcgtt ttatcgcgtc gtcaggcgtg gcggtggcgc cgcacgtcgt
     4141 gatcgagtcg tcggatgcgt tggcggcgct cgacgatgcg aagctcgagg cggtgctgcc
     4201 gggcattctc aagactgccc gcatgggcta tgacggtaaa ggccagatcc gcgtgcgcaa
     4261 cgcggaggaa gtgcgcgagg cgcatgcatc gctcgccggc gtgccttgcg tgctggaaaa
     4321 gcgcttgccg ctgaagttcg aggtgtccgc gctgattgcg cgcgcggcga gcggcgcgtc
     4381 ggtggtgtat ccgctggcgc agaacacgca tcgcgacggc gtgctgtcgc acaccatcgt
     4441 ccccgcgccg gacgcaagct cgacgctcgt cgggcaggca caacaggccg cgctgcaaat
     4501 cgccgacaag ctcggctatg tgggcgtgct gtgcgtcgag ttcttcatct tggaagacgg
     4561 atcgctcgtc gccaacgaaa tggcgccgcg cccgcacaac tccggccact acaccgtcga
     4621 tgcctgcgcc accagccagt tcgaacagca agtgcgcgcg atgaccggta tgccactcgg
     4681 cgacacgcgc cagcattcgc cggccgtgat gctgaatatc ctcggcgacg tctggttccc
     4741 ggacggcccg aagcgcgcgg cggtcacgcc gccgtggcac gaagtcgccg cgatgccggc
     4801 ggcgcgtctg cacctgtacg gcaaggaaga ggcgcgttgc ggccgtaaga tgggtcacgt
     4861 gaacttcacc gccgcgacgc tcgaagaagc ccgcacggct ggccgcgatt gcgcgcggct
     4921 cctgcacatc atcacgagtt gacgcgagcg ccatgccgga tcaaagcaaa cctgccgaag
     4981 gaggcgctgc aaacgccttg cctgtgagcg ccgagcagat cgggcacgcg gccgcgctgc
     5041 tctacgcggg tggtctggtc gcgttcccca cggagactgt ctacggactc ggcggtgacg
     5101 ccgagagtcc ggacgcggtc gcacgtattt atgcggccaa gggcaggccg gcgaatcatc
     5161 cggtgatcgt gcacctggcg ccgcagggcg atccgaacta ctgggtcgaa catttgcctg
     5221 ctgaggcgca gcgcttgatc gatgcgttct ggcctgggcc actcacgttg atcctgaagc
     5281 gcgcggcgcg aatcgatgcg gcggtgagcg gcgggcagga ttcggtgggg ctgcgctgcc
     5341 cgtcgcatcc ggttgcccag gctttgctgg aagcgttcag tgcgttgcgt ggcgggcatg
     5401 gcggtgtggc ggcgccgtcg gcgaatcgct ttggccatgt gagcccgacg actgcgcagc
     5461 atgtgcgcga agagttcggc ggcgcgattc atgtgctgga cggcggcgcg tcggacgtcg
     5521 ggattgagtc gacgattctg gatttgtcgc gtggatttcc ggcgctgtta aggcctggcc
     5581 gcgtcacgcc gcaagatatt gccgatgtgc tcggcgaggc gccgagattg ccggacggat
     5641 cggatgcgac tgcgccgcgc gcgtcgggga cgttgaaggc acattacgcg ccgcgcacgc
     5701 cgttggcttt gctgcctttt gatgcgttgc agagtttgct tgcggcgcgc cagggcgatg
     5761 agcgggttgc cttggtggcg cgggcgtccc gcgcagggga gtgggctcat gccgagggcg
     5821 tgcatttcat tgccgcgcct gaagatcctc acgtttatgc gcgcgagttg tatggcttgc
     5881 taagggcgct cgaccgcgcg gatgtgagcc ggattctgat tgagaagttg ccggacacga
     5941 tcgagtggat cgctgtgaac gatcggctgg gcagggccgc ggctgcattc gaagcgcagg
     6001 ggtaggacct gcctgattgg ttcggcttgc tcaccggcgc cgccacaat
//
LOCUS       DATASH010000975         2398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_362575,
            whole genome shotgun sequence.
ACCESSION   DATASH010000975 DATASH010000000
VERSION     DATASH010000975.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362575"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            582..1382
                     /locus_tag="VHV99_26025"
     CDS             582..1382
                     /locus_tag="VHV99_26025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX3638875.1"
                     /translation="MTSNQANPANPNGHGTAGEGVSAVTPTASPTFSPLYQQIKGLIT
                     QSLESGEWKPGEIIPSEVELAARFKVSQGTVRKAIEELAADNLLVRRQGKGTFVATHN
                     EDRAQFRFLRLLADDGAEHPHVSRLLECRRLRASADIARQLALKPADPVVLIKRLLQF
                     DGEVTVLDEIWLPGAVFRGLTLERLSEYKGPLYAMFETEFGTRMIRATEKIRAVAAEP
                     SVADLLHVPAGFPLLSVERVSYTYGDRPVEVRRGWYVTTGYYYQNDLS"
     gene            1540..1956
                     /gene="sdhC"
                     /locus_tag="VHV99_26030"
     CDS             1540..1956
                     /gene="sdhC"
                     /locus_tag="VHV99_26030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEX3638876.1"
                     /translation="MAEAVKKPRPEFRNIGIGQILTAYRLPLAGRVSILHRLSGGLLF
                     VFLPFLLYLFDQSLTSELSFEVFKGFLSNIIVKLITLVLAWAFLFHFCAGVRHLFMDT
                     SHGLTTKEKGKQTSIVVLVVSSLLTIAFALKLFGAF"
     gene            1961..2329
                     /gene="sdhD"
                     /locus_tag="VHV99_26035"
     CDS             1961..2329
                     /gene="sdhD"
                     /locus_tag="VHV99_26035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018439830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEX3638877.1"
                     /translation="MAANNRIGPKRLVVGAHYGLRDWLAQRITAAIMAIYTIILLAWF
                     FGARDFSYDGWASIFATQWMKLATFVTLLSLFYHAWVGIRDIWMDYVKPVGTRLFLQA
                     LTIVWLLACAGYAAQILWRV"
BASE COUNT          484 a          672 c          715 g          527 t
ORIGIN      
        1 ttggcgatgc ggaacagcag ggagtaagcg atttgacctg cggcgccggt gacggcaacg
       61 cgctttgcgg gcttagccat tgagaaatct ccaggacgat gcgttagacg ctagggaaaa
      121 cgccattcta tatgcgcgtt cagcgaagcg ttgtgaaaca cggcggaatt cacggttcgc
      181 gtacggcccc ggaatgtgcc gaagctgtgc ggcgtgctgc ggcaagaccg ccatgccgga
      241 aacctgtact gttgagcgga ggcggggcat gttgcgccgg gtagccgctg cacggtgcgg
      301 gctcctgcaa cagcgcgttt cggcaagcgt ctgaccaacg aaggcggcga atcgaaacct
      361 ggactgcgtg ggaacagcat ggtgcagagt agctgccggt gcattgcggc gctgtattgc
      421 atcgtgcaaa acagccgccg aaaaacccgc aaacccttgc tcgacaagga gtgtaggagt
      481 cgtcagacac aaagtcaaca ttatcttatg tcttatataa gacacatcta ttgcggggaa
      541 aaccctagac gcggcccggc cctttatgat gaaatgcgcg gatgacttcg aaccaggcga
      601 accccgcgaa tccgaacggc catgggaccg cgggcgaggg tgtatccgct gtcacgccca
      661 ctgcgtcgcc cactttcagc cccttgtatc agcagatcaa gggcttgatt acgcagagcc
      721 tcgaaagcgg tgaatggaaa cctggcgaga tcattcctag tgaagtcgaa ttggcggcca
      781 gattcaaggt cagtcaggga actgtgcgca aagcgatcga agagcttgct gccgacaacc
      841 tgctggtgcg ccgccagggc aagggcactt ttgttgcgac gcacaacgaa gaccgtgccc
      901 agtttcgctt cctcagatta ctggccgatg acggcgcgga acacccgcac gtcagccgct
      961 tgcttgaatg ccggcgcttg cgcgcgtcgg cggacatcgc ccggcaactc gctctgaagc
     1021 ccgccgatcc ggtggtgctg atcaagcgct tgctgcagtt cgacggcgaa gtcaccgtac
     1081 tcgacgaaat ctggttgccc ggcgcggtgt tccgcgggct cacgctcgag cggctgtcgg
     1141 agtataaagg tcccctctat gcaatgttcg agactgaatt cggcacgcgc atgatccgcg
     1201 cgacagagaa gatccgcgcg gtcgcagccg agccttcagt ggctgacctg ttgcatgtgc
     1261 cggcgggttt tccgctgctt tcggtcgaac gcgtgtccta tacatatggc gaccggccgg
     1321 ttgaggtgcg ccgtggttgg tatgtcacaa ccgggtatta ctatcagaac gatctgagct
     1381 gaatcgggat tataaaaggc gccgcgtccc acacgcgcgc ctgtttgaag gcgcctttat
     1441 cgcgcacgct gtaacggacc tgtcgtggtt ttcgctgcag cgcgatatga aaaggcgcta
     1501 aaattgcgga ttagtgtgac tacatagtag gggtctagca tggctgaagc cgtaaaaaaa
     1561 ccgaggccgg aattccggaa catcggtatc gggcagattt tgacggcata tcgtctccct
     1621 ctagcggggc gtgtgtcgat cttgcatcgc ttaagcggtg ggctgctttt tgtttttctt
     1681 ccgttcctgc tgtacctctt cgatcagagc ctgacttccg aacttagttt cgaagtcttc
     1741 aaaggtttcc tctccaacat catcgtcaag ctcatcacgc tcgttctcgc gtgggccttc
     1801 ctgttccact tctgcgcagg tgtgcgtcac ctgttcatgg acacaagcca cggcctcacg
     1861 accaaagaga agggcaagca aacctccatc gtggtgctgg tcgtctcgtc gcttctgacg
     1921 attgcatttg cgctcaaact cttcggagca ttctaaaaaa atggcagcta ataaccgaat
     1981 cggtccgaag cgtctcgtcg tcggcgcgca ttacggtctg cgcgactggc tcgcacagcg
     2041 catcaccgcc gccatcatgg cgatctacac gatcatcctg ctcgcgtggt tcttcggcgc
     2101 gcgcgatttc tcgtatgacg gctgggcgtc gatcttcgcc acgcaatgga tgaagctcgc
     2161 cacgttcgtc acgttgctct cgctgttcta tcacgcgtgg gtgggtatcc gcgacatctg
     2221 gatggactat gtgaagcccg ttggcacgcg cctgtttctt caggcgctga cgatcgtctg
     2281 gctgctcgca tgtgcgggct acgctgcgca gattctctgg agagtgtaaa agaatggctg
     2341 caatcaagaa ttctctgccg cgtcgcaagt ttgacgtggt tatcgtcggc gcaggcgg
//
LOCUS       DATASH010000976         1721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5508732,
            whole genome shotgun sequence.
ACCESSION   DATASH010000976 DATASH010000000
VERSION     DATASH010000976.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5508732"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1134
                     /locus_tag="VHV99_26040"
     CDS             <1..1134
                     /locus_tag="VHV99_26040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX3638878.1"
                     /translation="PPVTVLTAHATASNTIEAMRLGAFDHLTKPIGRADLAQVLQRML
                     ASVVESSGGVRTEEPEALIGSSEAMRTVQKTIGVLADSDATVLITGETGTGKEVVARA
                     IHDHGQRKRGPFVAVNCAAIPADLLESELFGHMRGAFTGASADREGAFRQAEGGTLFL
                     DEIGDMDIAMQAKILRALQERVVVPVGGKPVPINARVIAATHRDLVRSVRDGTFREDL
                     FYRLHVVPIHLPPLSERIADIVPLAEYFPNRATAGKRLSADAAAHLIRYGWPGNVREL
                     KNTTERAAVMVRGEYIHATDLAFLEDTGSTQGPIFDWPDEDLPSALARLEEMLIRRAL
                     RHSGNNRTDAARRLNVNRQLLYAKLKRYGIEVADSSREGGEGG"
BASE COUNT          309 a          534 c          561 g          317 t
ORIGIN      
        1 ccgccggtga ccgtgctgac cgcgcacgcc accgcctcca atacgatcga ggccatgcgt
       61 ctgggcgcct tcgatcatct gaccaagccg atcggccgcg ccgatctcgc ccaggtgttg
      121 cagcgcatgc tggcgagcgt cgtggaatcc agcgggggtg tccgcactga ggagcccgag
      181 gcgctgatcg gctcgagcga ggcgatgcgc accgtgcaga agacgatcgg cgtgctcgcc
      241 gatagcgacg ccacggtgtt gatcacgggc gaaacgggca ccggcaagga ggtggtcgcg
      301 cgcgcgattc acgatcacgg gcagcgcaag cgcgggccat tcgtcgcggt caattgcgcg
      361 gcgattccgg ccgatctgct ggaaagcgaa ctcttcggcc acatgcgtgg cgcattcacc
      421 ggcgcgagcg ccgatcgcga aggggcgttc cgccaggccg aaggcggcac cctgtttctc
      481 gacgagatcg gcgacatgga cattgccatg caggccaaga tcctgcgcgc gttgcaggaa
      541 cgcgtcgtgg tgccggttgg cggcaagccg gttccgatca atgcgcgggt gatcgcggcg
      601 actcaccgcg atctggttcg cagtgtgcgg gacggcacgt ttcgcgagga tctgttctac
      661 cggctgcatg tggtgccgat ccacttgccg ccgctgtccg aaaggatcgc cgacatcgtg
      721 ccgctcgccg aatattttcc gaaccgcgcg acggcgggca aacgcctgtc cgcggatgcg
      781 gcggcgcatc tgatccgcta tggctggccc ggcaacgtgc gcgaactcaa gaatacgacg
      841 gagcgcgccg ccgtgatggt gcgcggcgaa tacattcacg cgaccgatct ggcgttcctg
      901 gaggatacgg gatcgacgca gggcccgatc ttcgactggc cggacgagga cctgccctcg
      961 gcgctcgcgc ggctcgagga aatgctgatt cgccgcgcgt tgcggcacag cggcaataac
     1021 cgcaccgatg ccgcgcggcg gctcaatgtg aaccgccagc tgctgtacgc gaagctcaag
     1081 cgctatggga tcgaggtggc cgactcgtcg cgggagggtg gcgagggagg gtagtcgtca
     1141 aggcgaacgg ctcgctagac gtttgcgcgc tcgcggctga ggtttagcgg tgcatctctc
     1201 tttgcggtcc atagaaatca aaaacggtca gttcacgcgg cttattacgc agcgctgaat
     1261 taagccggac atcgtctgct tcgcaggcat tcgacgtgac ttgcggcgcg cgaatcgtga
     1321 tgttttttga tcggccgctc gttgagcaaa tgccgttcga atcaccctgt ttcgccgcgt
     1381 gacgcggtag cagtaaggct ctccacttcg tccgggtcga agcgaacgca ccgcttgcgt
     1441 cacacaaaaa aaagcgtcgg gcgcgttccc tgtcccacgt ccaggcgatt taaagaacct
     1501 ccgtcaatcc cggcaatgcg ggccagcgca catagcgatg catgagccgg tcatctcaat
     1561 cccgcgacag atccgcctct acgccggctt tcgtctcaag gttgcgacat gtcggtccac
     1621 ggctcgcaaa agcgacttct tcgcacgacg tcgcggcatg tttgaccgga gtattcgtca
     1681 atccattgcc agtcagcgag gcttagggtt ttccttcatt c
//
LOCUS       DATASH010000977         7998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5113370,
            whole genome shotgun sequence.
ACCESSION   DATASH010000977 DATASH010000000
VERSION     DATASH010000977.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7998
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5113370"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2079
                     /locus_tag="VHV99_26045"
     CDS             <1..2079
                     /locus_tag="VHV99_26045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEX3638879.1"
                     /translation="RLIVSTLFAEPFRALHVSRLAPTYSQLIATLAQAAQATMQHDGT
                     GTDTSPHIALLTPGPFSETYFEHVFLARYLGVTLVEGKDLTVRDDMLYLKTLAGLERV
                     HVVLRRLDDAFCDPVELRADSSIGVPGLLQVMRAGKVIVSNVPGSGFVESPALHGFLP
                     GIAEVLLDEDLVLPSVATWWCGEKAARNHAFARLDEAFIVPTWPVAARDAPPGIEQGR
                     QRLATWRERIEAMPDAYTIQQPQRFSCTPRYEDGTIGRRPSVLRVYAIADVNGGWHVM
                     PGGFTRMAAERQATVSMQYGGSSVDTWVLSSQPTSTFTLLPSPMKPADLARKHRTVSS
                     RAAENLFWAGRYGERAENNVRLLRLILGSLEGNDADAMFPTLVELALHCGLVQSGDLF
                     SPHSPQAFERALVANLSESTGAASIGQNLNCQARSSGEVRGRLSNDHWRTILAARNDF
                     RDALQKLMPAAGLSGNGNGNGSASGSSQGSANGHGRAERSERYDRYDRVTLMNALEHL
                     SVQLSAISGAQGDRMTRDEAWRLLFVGRHIERVSAMTSFLRVVADKGQLATPAGFDLL
                     LQLFDSTLTYRSLYPGRFEVPALLDLLVIEPTNPRGIYGVYERLRKKLDEIAVAAGSM
                     RHRPFAELMLPAASLPTLESLCATDGNGSYVDLIVVCDQIGGFVGAAAHEISARYFSH
                     ASTVASQVWS"
     gene            2076..2966
                     /locus_tag="VHV99_26050"
     CDS             2076..2966
                     /locus_tag="VHV99_26050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEX3638880.1"
                     /translation="MKHAPTVLSVSHRTTYRYSTYVETAQHLATIRPIACAWQRVVSH
                     TEKIEPEPSYLNSRIDAFGNDVLYFALDAPHERLQLVSETTVALMPRWTDLDPEATPE
                     WEEVADALRFWVGGEFRPEAEFCFASPNIVPRPSLRAYALPSFPPGMPIAAGAIDLMH
                     RIHEDFAYKPSATMFDTPAERAFELKSGVCQDFAQVMIGCLRSLGLPARYVSGYLRND
                     PPPGQPRLIGADASHAWVSVHCPEVGWIDLDPTNDVLADMDHVTLAIGRDYSDVSLLR
                     GMILGGGAHRVDVGVTVLAL"
     gene            3216..3965
                     /locus_tag="VHV99_26055"
     CDS             3216..3965
                     /locus_tag="VHV99_26055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX3638881.1"
                     /translation="MNAEQSVTGAERVLLVLAALASHGKAMSVKDLLAVTGLAQSTLY
                     RQIALLKRWGFVAENAGHYAPGPISLQLALGFDVNSLLVEASRSEMQQLARASQESIG
                     LVVAVKHQVMCLEMVDSQHSLRCSFEKGRAVPLKAGASAKSLLAFMADKARTEVLDTV
                     FDNDPAGRAAIEAELEQIRAQGYAVSDSEVDPGVWGVSAPIFHRSGRGVGSSASITLM
                     APSTRAIGRESQFIDGTLRTARAISGLMQTD"
     gene            4108..4863
                     /locus_tag="VHV99_26060"
     CDS             4108..4863
                     /locus_tag="VHV99_26060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX3638882.1"
                     /translation="MNLNRLLSLACVTVAVTFAGFSGAASAQTPDVLNVATDATFPPM
                     EYTENGARTGFDIDLMNALAKAMGKRVQWTDIDFKGLIPGLIAHRFDAAISGIYITDD
                     RAKVVDFTDSYYAGGLVALVKSDSPVKSVTDLNGKKVSVQVGTKSVNFLRDNFPQINR
                     VEVEKNQEMFDLVGIGRADAAVTGKPAAYQLVKTRPGFRVLDKQLTTEAYGIAVRKDE
                     PQLKAAFNTALAKIKADGTYAAILHKWFGATAQ"
     gene            4881..5549
                     /locus_tag="VHV99_26065"
     CDS             4881..5549
                     /locus_tag="VHV99_26065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX3638883.1"
                     /translation="MELDFSPVIAGWPDIMHGALVTVEVTAAALALSCVLGLLIGIGR
                     LTPKRRVVYAFCTAYLTFFRGTPLLVQLFLLFFGLPQFGILLPAFVCGMLGLGLYSAA
                     YVSEIVRGAIQSVDRGQMEAARSIGMSSGQAMRAIILPQAIVRMIPPLGNEFIALIKN
                     SALVSLLTIDDLMHEGQKIISVSYRSLEVYLAIALVYLVLTQVTNYALHRVERRLRAG
                     GMVQ"
     gene            5546..6322
                     /locus_tag="VHV99_26070"
     CDS             5546..6322
                     /locus_tag="VHV99_26070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX3638884.1"
                     /translation="MNTVAKVVEPIVSIRGLTKSFGSHTVLNGIDFDIQPQQVVVVIG
                     PSGSGKSTFLRCCNGLEEAEGGTIDICGHRLVDQGAMLKERSLNALRTEVGMVFQSFN
                     LFPHLSVLHNITVGPRMLRGASKAEAEAAAIALLEKVGLAHKAHVMPASLSGGQKQRV
                     AIARALAMQPRVMLFDEPTSALDPELVGEVLQVMKLLASEGMTMVVVTHEMGFAKEVA
                     DVVVVMDGGVIVEAGPPAEIFSAPSQPRTRAFLQAVLSRA"
     gene            6319..7437
                     /locus_tag="VHV99_26075"
     CDS             6319..7437
                     /locus_tag="VHV99_26075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HEX3638885.1"
                     /translation="MRVPFDEKVWSGRSDDGEPGDTRRVFNQVVPFAFGRVERHENEL
                     SVAPRIGGDAPVIVGFGSDEGVRRNQGRTGAAHAPKELRRVLAGLPAKTSMTALADAG
                     DVVCDDGDLEGAQAELANVVSEVLANGGRPLVFGGGHEVAWGTYCGLRMHLERVAMGT
                     GTGTGTGRGADVDVDVVAVAGDDRGIEVDTDAISTATAAAAKATRKLLIINFDAHFDL
                     RQKRPANSGTPFDQIANDCAERGVPFNYACFGVSDLGNTASLFAHAEELGVRYVLDID
                     MQEPQLPQRLDDLEKLLDLADDVYLTIDLDVLPAATAPGVSAPAALGVPLSVVEAMVQ
                     RVRASGKLRVMDIAEYNPSLDQDKRTARAAARLAYRLL"
     gene            7747..>7998
                     /locus_tag="VHV99_26080"
     CDS             7747..>7998
                     /locus_tag="VHV99_26080"
                     /inference="COORDINATES: protein motif:HMM:NF024418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR domain-containing protein"
                     /protein_id="HEX3638886.1"
                     /translation="MNRKTYRLVYSRLRGMLIAVEETATATGKETGQTTVCNGANSAS
                     HSSLLALRQIAFAALALVGAFPSWSGAQIVPGGAHAPSVV"
BASE COUNT         1435 a         2322 c         2615 g         1626 t
ORIGIN      
        1 cgtctgatcg tctcgacgct gttcgccgaa ccgttccgcg cgctgcatgt gagccgcctc
       61 gcgccgacct attcgcaact gatcgccacg ctcgcgcagg ccgcacaagc cacgatgcag
      121 cacgacggta cgggcaccga tacttcgccg catatcgcgt tgctcacgcc ggggccattt
      181 agcgaaacct atttcgagca cgtctttctc gcgcgctatc tcggcgtcac gctggtggaa
      241 ggcaaggacc tgaccgtgcg cgacgacatg ctgtatctga agacgctcgc cggtctggaa
      301 cgtgtgcacg tcgtgctgcg ccgtctggat gatgccttct gcgatccggt cgaactgcgg
      361 gcggattcga gcatcggcgt gccgggcctg ctgcaggtga tgcgcgcggg caaggtgatc
      421 gtctcgaacg tgccgggttc gggctttgtc gagtcgccgg ctttgcatgg ctttttgccg
      481 ggcatcgccg aagtgctgct cgacgaagac ctcgtgctgc cgagcgtggc gacctggtgg
      541 tgcggcgaaa aggcggcgcg caaccacgcg ttcgcgcgtc ttgacgaagc gttcatcgtg
      601 ccgacgtggc cggtcgccgc acgcgatgcg ccgcccggga tcgaacaggg caggcagcgc
      661 ctcgcgacgt ggcgcgaacg tatcgaagca atgcccgacg cctacacgat ccagcagccg
      721 cagcgttttt cctgcacgcc gcgttacgag gacggcacga ttggccgccg tccgtcggtg
      781 ctgcgcgtct atgcgatcgc ggatgtgaac ggcggctggc atgtgatgcc gggcggcttc
      841 acgcgcatgg ccgccgagcg tcaggcgacg gtgtcgatgc aatacggcgg cagcagcgtc
      901 gatacgtggg tgctgtccag tcagccgacg tcgaccttca cgctgctgcc ttcgccgatg
      961 aaacccgccg acctcgcgcg caagcatcgc accgtgtcga gccgcgcggc ggagaatctg
     1021 ttctgggccg gacgttacgg cgaacgcgcg gaaaacaatg tgcggctgtt gcgcctgatt
     1081 ctcggctcgc tggaaggcaa tgatgccgac gcgatgttcc ccacgctggt cgagctcgcg
     1141 ctgcattgcg gtctcgtgca atccggcgat ttgttctcgc cgcattcgcc gcaggcgttc
     1201 gagcgcgcgc tggtcgcgaa tctaagtgag agcaccggcg ctgcgagcat cggtcaaaac
     1261 ctcaattgcc aggcgcgttc gagcggcgaa gtgcgtgggc gtttgtcgaa cgatcactgg
     1321 cgcacgattc tcgcggcgcg caacgacttc cgcgatgcgt tgcagaagtt gatgccggct
     1381 gcgggtttga gcggcaatgg caatggcaat ggtagcgcta gtggttcatc gcaaggcagc
     1441 gcgaatggtc acggccgcgc ggagcgcagt gagcgctacg accgttatga ccgcgtgacc
     1501 ctgatgaatg cgcttgaaca cctgtcggtg cagttgtcgg cgatcagcgg cgcacaaggc
     1561 gaccgcatga cacgcgacga agcatggcgt ctgctgttcg tcgggcggca catcgaacgg
     1621 gtatcggcga tgacgtcgtt cctgcgcgtg gtcgcggata agggccaact cgcgacgccg
     1681 gccggcttcg atctgctgct gcaattgttc gacagcacgc tgacgtatcg ctcgctgtat
     1741 ccgggccgtt tcgaagtgcc ggctttgctc gacctgctgg tgatcgagcc aaccaatccg
     1801 cgcggcatct acggtgtcta tgagcgcctg cgcaaaaagc tcgacgagat tgccgtggcc
     1861 gcgggcagca tgcggcatcg tccgtttgcg gaattgatgt tgcctgctgc ttcgttgccg
     1921 acgctcgaat cgctgtgcgc aaccgacgga aatggttcat acgtcgatct gattgtggtg
     1981 tgcgaccaga ttggcggctt tgtcggcgcg gcagcgcatg agatcagcgc gcgttatttc
     2041 agtcatgcca gtacggtggc ttcccaggtg tggtcatgaa gcacgcaccg acggtgttgt
     2101 ccgtctcgca tcgcacgact tatcgctact ccacgtatgt ggagaccgcc cagcatctcg
     2161 cgacgatccg gccgatcgcc tgtgcgtggc agcgggtggt gtcgcacacc gaaaagatcg
     2221 aaccggagcc gtcgtatctg aatagccgta tcgatgcatt cggcaacgac gtcctgtatt
     2281 ttgcgctgga cgccccgcac gaacggctgc aactcgttag cgaaaccacg gtggcgctca
     2341 tgccgcgttg gaccgacctc gaccccgagg cgacgcctga atgggaggag gtggcggacg
     2401 cgctgcgttt ttgggtgggt ggcgagttcc ggcccgaggc ggagttctgc tttgcgtcgc
     2461 cgaatatcgt gccgcggcct tcattgcgcg cttacgcgtt gccgagcttt ccgcccggca
     2521 tgccgatcgc ggcgggtgcg atcgatctga tgcacaggat tcacgaagac ttcgcgtaca
     2581 aaccttcggc gacgatgttc gatacgccgg ccgagcgcgc tttcgaattg aagagtgggg
     2641 tatgccagga cttcgcgcag gtgatgatcg gctgcttgcg ttcgctgggt ttgccggcgc
     2701 gttacgtgag cggctatttg cgcaacgatc cgccgccggg gcaacctcgt ttgatcggtg
     2761 ccgatgcgtc gcatgcgtgg gtgtctgtgc attgcccgga agtcggctgg atcgatctcg
     2821 atcctaccaa tgacgtgctc gccgatatgg atcatgtaac gctcgcgatc gggcgcgatt
     2881 acagcgacgt gtcgttgctg cgcgggatga ttctgggggg tggggcgcat cgggtggatg
     2941 tgggggtgac ggtgttggcg ctttagcttg cggttttaat tcgctgcttc tgcacgcggt
     3001 gctattcccg ccgcgttttc ccgcttcacc cgttagggac accgccttct ggtcagacag
     3061 attctcccca cgcggtacat ctgcagtcgc acgccttttc tccccctttc ggggaaaacc
     3121 ctctccctcc attcccactc cccgggaatc ccatttttga aatccgagaa cgttctggca
     3181 tcctgattcc gttattcatc gtgggtagct tcatcgtgaa cgcggaacag agtgtgacag
     3241 gggctgaacg ggtgctgctg gtgctggcgg cgcttgccag tcatggcaag gccatgtcgg
     3301 tcaaggatct gctcgccgtc accggtctcg cgcaaagcac gctgtatcgg cagatcgcct
     3361 tgctcaagcg ctggggtttc gtcgcggaaa acgcgggcca ttacgcgccc ggcccgatca
     3421 gtttgcaact cgcccttggc ttcgacgtga attcgctgct cgtcgaagcg agccgcagcg
     3481 aaatgcagca gctcgcacga gcttcgcagg aaagcatcgg ccttgttgtc gcggtcaagc
     3541 atcaggtgat gtgcctggaa atggtcgaca gccagcactc gttgcgctgc tcatttgaaa
     3601 aaggccgcgc ggtgccgctg aaagccggcg cttcggcgaa atcgctgctg gctttcatgg
     3661 ccgacaaggc gcgcactgag gtcctcgata cggtgttcga caacgacccc gccggccgtg
     3721 ccgcgatcga agccgagctc gaacagattc gcgctcaagg ctacgccgtc agcgatagcg
     3781 aagtcgatcc gggcgtgtgg ggcgtgagcg ccccgatctt tcatcgcagc ggacgcggcg
     3841 tgggcagcag cgcgtcgatc acgctgatgg cgccgtccac gcgcgccatc ggccgcgaaa
     3901 gccaattcat cgacgggacg ctacgtacgg cccgcgccat ttccgggctt atgcaaaccg
     3961 attgatcggg cagggccgct cgcggccgta ttcgatctgg ataccgaacg aactgaacga
     4021 accaagccag ccaaaccaac caagccagcc aaatcaacca agccagccag ccaaaccaac
     4081 caagccacaa ggagccctcc catgaaactg aatctgaatc gcctgttgtc cctcgcatgc
     4141 gtcacggtcg cagtgacgtt tgccggcttt tccggcgcgg cgtcggcgca aacgccggac
     4201 gtgctgaacg tcgccaccga cgcgaccttc ccgccgatgg aatacaccga aaacggcgca
     4261 cgcacaggat tcgacatcga cctgatgaat gcgctcgcga aagcgatggg caaacgcgtg
     4321 caatggaccg acatcgactt taaaggcctg attccgggct tgatcgcgca tcgcttcgac
     4381 gcggcgatct ccggcatcta catcaccgac gaccgcgcca aggtagtcga cttcaccgac
     4441 tcgtactacg caggcggcct cgtcgcgttg gtgaaaagcg attcgccggt caagtcggtc
     4501 actgatttga acggcaagaa agtgtcggtc caggtgggca cgaagtcggt caacttcctg
     4561 cgcgacaatt tcccgcagat caaccgcgtg gaagtggaga agaatcagga aatgttcgac
     4621 ctggtcggca tcggccgtgc tgacgccgct gtgaccggca agccggccgc ctatcagctg
     4681 gtaaagacgc gccccggctt ccgtgtgctg gacaaacagt tgacgaccga agcctacggc
     4741 atcgccgtgc gcaaggacga accgcaactg aaagcagcct tcaacacagc actggcgaag
     4801 atcaaggccg atggcacgta cgcggcgatt cttcacaagt ggttcggcgc caccgcgcaa
     4861 taaacggctg agcagacaca atggaactcg atttttcgcc ggtgattgcc ggttggccgg
     4921 acatcatgca tggcgcactg gtgacggtcg aagtgaccgc cgccgcgctc gcactgagct
     4981 gtgtgctggg cctgttgatc ggtatcggcc ggctcacgcc gaagcggcga gtcgtgtacg
     5041 ccttctgcac cgcgtacctc acgttcttcc gcggtacacc gttgctcgtg caactattcc
     5101 tgttgttctt cgggttgccg caattcggca ttctgttacc ggctttcgtg tgcgggatgc
     5161 tggggctcgg cctgtattcg gcggcgtatg tgtcggagat cgtgcgcggc gcgattcagt
     5221 cggtggatcg cgggcagatg gaagcggcac gctcgatcgg catgtcgtcg gggcaggcga
     5281 tgcgcgcgat cattttgccg caggcgatcg tgcggatgat cccgccgctt ggcaacgagt
     5341 tcatcgcgtt gatcaagaac tcggcgctgg tgtcgctgct gacgatcgac gacctgatgc
     5401 acgaaggtca gaagatcatc agcgtgtcgt accgttcgct cgaagtgtat ctggccatcg
     5461 cgctggtgta tctggtgttg acgcaggtga ccaattacgc gctgcatcgt gtggagcgcc
     5521 gcttgcgtgc aggagggatg gtgcaatgaa taccgtggcc aaagtggttg aacctatcgt
     5581 cagcattcgt gggctaacca aatcgttcgg ctcgcatact gtgttgaacg gcatcgactt
     5641 cgacattcag ccgcagcagg tggtggtggt gatcgggccg agcggctcgg gcaagagtac
     5701 gttcctgcgt tgctgcaatg ggctcgaaga ggctgaaggc ggcacgatcg atatctgcgg
     5761 gcatcggttg gtcgatcagg gcgcgatgct caaggagcgc tcgctgaatg cgctgcgcac
     5821 tgaagtgggc atggtgtttc agtcgttcaa tctgtttccg catctgtcgg tgctgcataa
     5881 catcacagtc ggtccgcgga tgttgcgtgg cgccagcaag gccgaagccg aagcggctgc
     5941 gatcgcgttg ctggaaaaag ttggcctcgc gcacaaggcg catgtgatgc cggcgagttt
     6001 gtcgggcggt cagaagcagc gcgttgcgat tgcccgcgcg ttggcgatgc aaccgcgcgt
     6061 gatgctgttc gacgagccca cgtccgcgct cgatcccgag ttggtcggcg aagtgctgca
     6121 ggtgatgaag ctgctcgcga gcgaaggcat gacgatggtg gtcgttacgc acgaaatggg
     6181 tttcgcgaag gaagtcgccg acgtggtggt ggtgatggac ggcggcgtga tcgtcgaggc
     6241 gggaccgcct gctgagattt tctcggcgcc gtcgcagccg cgcacgcggg ccttcctgca
     6301 ggcggtgctg tcgcgcgcat gagagttcca ttcgacgaga aggtgtggag cggccgttcc
     6361 gatgatggcg agcccggcga tacgcggcgt gttttcaatc aggtggtgcc gtttgcgttc
     6421 ggccgcgtgg agcggcatga gaatgagttg agcgttgcgc cgcgcattgg aggcgatgcc
     6481 ccggtgatcg tcggttttgg ctccgatgaa ggcgtgcggc gcaatcaagg gcgtacgggc
     6541 gcggcacacg ccccgaagga actgcgtcgc gtgctggcgg gtttgccggc gaagacctcg
     6601 atgaccgcgc tcgctgatgc gggcgatgtg gtgtgcgacg acggcgatct cgaaggcgcg
     6661 caggcggaac tcgcgaacgt ggtgagcgaa gtgctcgcga acggcggccg gccgttggtg
     6721 ttcggcggcg ggcatgaggt tgcgtggggg acgtattgcg gtttgcgcat gcatctggag
     6781 cgtgtggcta tgggcacagg cacaggcaca ggcacaggca ggggcgcgga cgtggatgtg
     6841 gatgtggttg cagttgcagg tgacgacaga ggcatagagg tagacacaga tgcgatttcg
     6901 acagcaaccg ctgccgcggc caaggcaacc cgcaagctgc ttatcatcaa cttcgacgcc
     6961 cacttcgatc tgcgccagaa gcggcccgct aattcgggca cgccgttcga tcaaatcgcg
     7021 aacgattgcg ccgaacgagg tgtgccgttc aactacgcct gctttggtgt cagcgatctg
     7081 ggcaacaccg cttcgctgtt cgcgcatgca gaggaactgg gcgtgcgcta cgtgctagac
     7141 atcgacatgc aggaaccgca attgccacag cgtctggacg atctcgagaa gttgctggat
     7201 ttagcggacg acgtctacct aaccatcgat ctcgacgtgc tcccggctgc cacggcaccg
     7261 ggcgtatcgg cgccggccgc gttaggcgtg cccttgtccg tggtcgaagc gatggtgcag
     7321 cgcgtgcgcg cgtcgggcaa gctgcgcgtg atggatattg cggagtacaa cccgtctctc
     7381 gatcaggaca agcggacggc gagagcagcg gcgcgattgg cgtatcggtt gctttgactg
     7441 atcatgatcg agttgtactg actcgcgcat gctgcacaaa aagcggctcg ccggattatc
     7501 tcggcgagcc gtttttctat ttcagccgcg tgttcatgct gtcccgtcct gtgttttcct
     7561 tacacatact ccgcgcgtgt tttgtgccag tcagggatgc gttatatcgc ggtagggctc
     7621 gataggatta atcttattta gtagaaattg ttgcattgtt gctttcttga caattattga
     7681 cgcattttca agcgtcctcc gatatttttg cgatctctta taaatcagta agacaaaaca
     7741 aaaagaatga atcggaagac gtacaggctg gtttacagcc ggctgagagg aatgctgatt
     7801 gccgtggagg aaaccgccac ggcaacggga aaggaaaccg gccagaccac ggtttgcaac
     7861 ggcgctaatt cggccagcca ctcatcgcta ctcgcactgc ggcagatcgc gttcgccgcg
     7921 ctggcgctag tgggtgcgtt cccctcatgg tcaggcgcac agattgttcc gggcggcgcg
     7981 cacgcgccgt ctgtcgtc
//
LOCUS       DATASH010000978         3983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_759159,
            whole genome shotgun sequence.
ACCESSION   DATASH010000978 DATASH010000000
VERSION     DATASH010000978.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3983
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759159"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VHV99_26085"
     CDS             complement(<1..430)
                     /locus_tag="VHV99_26085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006053033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX3638887.1"
                     /translation="MRLFLHSVLLALCAAVGAAASASTVISRSFHSEALGRDWAYTIY
                     LPTGYRHDAVRIPVLYLLHGNNGDANDWLTQGHLQTTADALIDRKEMPPVAIVMPQGG
                     TDWYVDRKEKMETAFFDDLLPEIETRYAVATQRGGRMIGGV"
     gene            complement(560..1669)
                     /locus_tag="VHV99_26090"
     CDS             complement(560..1669)
                     /locus_tag="VHV99_26090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OpgC domain-containing protein"
                     /protein_id="HEX3638888.1"
                     /translation="MQKSQSRLIELDFFRGLVLLIIVVDHIGGSILSRVTLHAYALCD
                     AAEVFVFLGGFATATAYAALAERRNEASARSRFLRRSLEIYRAFLITAGLMLLVSAVL
                     TAFSINGPNLATADLDDLIAAPVAALRDILLFRRQPYLASVLPMYAFFALLVPMILPL
                     ARRKPWLLLAGSVALWAGAPAIDAYLPAAPDMHWDFNPFAWQLLFVLGVLARCQPVYQ
                     RLSAHRLGWLISVLAFAVVAAAAYYKLFIEREPLDGSLKQNLWYLRAINFLAIAWLVA
                     NLIQLGWARKLAQWMPWVGMIGRQGLLCFIAGAVISLVVDSVLYDATDGYLNYPLGLV
                     ADAFAIGALFAVARASEPLKRCLTRLWSLRLGTAP"
     gene            complement(1731..2342)
                     /locus_tag="VHV99_26095"
     CDS             complement(1731..2342)
                     /locus_tag="VHV99_26095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638889.1"
                     /translation="MKISLHSSNGTTRSDRFLLTLLEAFIGPGALSGNPHMPLPRRPS
                     GVASISQNVAKSPLRATPSSFAALIQVNDAAPAALKPAVARTAPPRHDASNRCLFEWL
                     ARGCPGSGWTVVSSTGRLGFVMGRNIFESFRGAAAELRTVSRVIFTGLCKRTARGRFK
                     RRCKWPPAGGRPVDGHPVGAAVHGVAILQGGRPPQYLIPFQKA"
     gene            2341..3513
                     /locus_tag="VHV99_26100"
     CDS             2341..3513
                     /locus_tag="VHV99_26100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX3638890.1"
                     /translation="MNKQVFALAVSTALSAASLATVSAPAGAQTSVTLYGVLDEGINY
                     TNNVGRGHVYELASGDAQGSRWGLKGAEELGGGLKAIFQLENGFDVSSGRLNQGGRMF
                     GRQAFVGLSSEPYGTLTFGRQYDSVVDYLAQTTANGNWAGLLFSHPYDNDNTDNTFRL
                     DNSVKYTSPSLSGFQFGGVYSFSNDTGFANNRAYSFGGQYAYGGLLVAAAYLQADNPG
                     NGSNGAITANDASFIAARMRVFGGGITYTFGPATAGFVYTNSNYLDPTANGYLGVTPL
                     VPPGVLLNSLKYQNFEVNGKYQISPMLFVGAQYVYTMETYDASSGGVKPKIHSFGLMA
                     DYNLSKRTDVYIQGEYQRVTGDPTYSILDDAFTPGTQSPSSTSKQVVVRAAIRHKF"
     gene            complement(3599..3898)
                     /locus_tag="VHV99_26105"
     CDS             complement(3599..3898)
                     /locus_tag="VHV99_26105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638891.1"
                     /translation="MGGFFFGALVAAVVMILLCNAGEFSPFVAKPHALEQVIVTALIS
                     LVIVDAKPLAYKTVWRERRCSFVLDEDLSATIRGRTFGIPAGVVIGILIQNYVMN"
BASE COUNT          842 a         1222 c         1240 g          679 t
ORIGIN      
        1 acacgccgcc gatcatccgc ccgccacgtt gcgtcgcgac cgcatagcgc gtttcgatct
       61 caggcaacag atcgtcgaag aacgccgtct ccatcttctc cttacgatcg acataccagt
      121 ccgtaccgcc ttgcggcatc acgatcgcga ccggcggcat ttccttgcga tcgatcaacg
      181 cgtcggcggt ggtttgcaga tggccctggg tgagccagtc gttggcgtcg ccgttattgc
      241 cgtgcagaag atagagcact ggaatgcgga cggcgtcgtg gcgatagccg gtgggcaaat
      301 agatcgtgta ggcccagtcg cggccgagcg cctcggagtg gaagcttcgg ctgatgacgg
      361 tgctggcgct cgccgccgcg ccgacagcag cgcatagcgc aaggagtacg gagtgtagga
      421 atagacgcat gtcagtgtaa gaggccgcca cgcaggtcgc gcgcggccgt catgctatca
      481 gcatcggcgg ggaaatcggc aagcggcagt gagccaggcg gcaaaagccc gcgcttgcgt
      541 gcgcaggctg cccgctcatt cagggcgccg ttccgagccg caagctccaa aggcgcgtca
      601 agcaccgctt gagcggctcc gacgccctcg cgacggcgaa cagcgcgccg atcgcaaacg
      661 catccgccac cagcccgagg ggatagttca gatagccgtc ggtcgcgtca tacagcaccg
      721 aatcgacgac cagcgaaatc accgcgcccg cgataaagca cagcagcccc tgacggccga
      781 tcatgccgac ccacggcatc cattgagcga gctttctggc ccagccgagc tggatcaggt
      841 tcgcgacgag ccacgcaatc gcgagaaaat tgattgcgcg caggtaccag aggttttgct
      901 tgaggctgcc gtcgagcggc tcgcgttcga taaacagctt gtaataggcc gcggccgcca
      961 ccacggcgaa cgccagcacg ctgatcagcc agccgagacg gtgcgcgctg agccgctgat
     1021 aaaccggctg gcaacgtgcc agcacgccga gcacgaacag cagctgccag gcaaacgggt
     1081 tgaaatccca gtgcatgtcc ggcgcggcgg gcaggtaggc gtcgatagcg ggtgcgccgg
     1141 cccacagtgc gacgctgccg gccagcagca gccacggctt cctgcgcgcg agcggcagga
     1201 tcatgggcac gagcagcgcg aagaacgcat acatcggcaa cacggaagcg agatacggct
     1261 gacgccggaa tagcaggatg tcgcgcaggg cggccacggg ggccgcgatc agatcgtcga
     1321 gatccgcggt ggcgagattg ggcccgttga tactgaacgc tgtcagcacg gcgctcacca
     1381 gcagcatcaa acccgcggtg atgaggaagg cgcgatagat ttcgagcgag cgccgcaaga
     1441 agcggctgcg cgcgctcgcc tcgttacgcc gttcggccag cgccgcgtag gctgtggcgg
     1501 tggcgaagcc gccgaggaat acgaagactt cggccgcatc gcacagcgca taggcatgca
     1561 gcgtgacgcg cgacagaatg ctgccgccga tgtgatcgac gacgatgatc aggaggacca
     1621 gcccgcggaa aaaatcgagc tcgatcaagc gggattgcga cttttgcatt gtgtggcgcc
     1681 aaaagcagcc ccgtgcgacg gcgcgctagt tcagcgcggc aggcgtctca tcaggctttt
     1741 tgaaatggga tcaggtactg cggcgggcgg ccgccctgga ggatagccac cccatgaacc
     1801 gccgccccca ctgggtgacc atccacgggg cggccacctg cgggcggcca cttgcatcgc
     1861 cgcttgaacc ggccgcgcgc ggtgcgcttg cagaggccag taaaaatgac ccgactgacg
     1921 gtgcggagtt ccgctgcagc gccgcgaaag ctttcgaata tatttcttcc catgacaaag
     1981 ccgaggcggc ctgtgctgga tacaaccgtc cacccgcttc ccggacaacc gcgcgccagc
     2041 cactcgaaca ggcagcgatt gcttgcatcg tggcggggcg gcgcagtcct ggcgacagcc
     2101 ggcttaagtg cggccggcgc ggcgtcgttt acctgaataa gcgccgcgaa agaagagggc
     2161 gttgcacgca gtggagattt cgcaacgttt tgagatatgg acgcgacacc cgatggacgg
     2221 cgaggtaacg gcatgtgagg atttcctgaa agcgcgccgg ggccgataaa cgcctccaga
     2281 agcgtcaaaa gaaagcggtc ggatcgagtt gtcccattac tcgagtgcag ggagatcttc
     2341 atgaacaagc aagtgttcgc gctggctgtc tccaccgctt tgtccgccgc ctcgttagct
     2401 actgtctccg cgcctgccgg tgcccagacg agtgtgacgc tctacggcgt gctcgatgaa
     2461 ggcatcaact acaccaacaa cgtcggccgc ggccatgtct atgaactcgc aagcggcgat
     2521 gcgcaaggca gccgctgggg cttgaaaggc gccgaggaac tcggcggcgg cctgaaggcg
     2581 atcttccagc tggaaaacgg cttcgacgtc agctcagggc ggcttaacca gggcggccgg
     2641 atgttcggcc gtcaggcgtt cgtcggcttg agttcggagc cctacggcac cttgaccttc
     2701 gggcgtcagt acgattcggt ggtcgactat ctggcgcaga ccacggccaa cggcaactgg
     2761 gccggtttgc tgttctccca cccgtacgat aacgacaaca ccgacaacac attccgactc
     2821 gacaacagcg tcaagtacac cagcccgtcg ctctcgggct ttcagtttgg cggcgtgtac
     2881 agcttcagca atgacacggg cttcgccaac aatcgcgcgt acagtttcgg cggccagtac
     2941 gcctacggcg gactgctggt ggccgcggcg tatctgcagg ccgacaaccc cggcaacggc
     3001 tcgaacggcg cgatcaccgc aaacgacgcc agctttattg ccgcccgcat gcgcgtgttc
     3061 ggcggcggca tcacttatac cttcggcccg gctactgccg gcttcgtgta caccaactcg
     3121 aactatctgg atccgaccgc caacggctat ctcggcgtga cgccgctcgt accgcccggt
     3181 gtgctgctga actcgctgaa atatcagaac ttcgaggtga acggcaagta ccagatttca
     3241 ccgatgctgt ttgtgggggc ccagtacgtg tacacgatgg aaacctacga cgcctcgagc
     3301 ggcggcgtga agccgaaaat tcattcgttc ggcttgatgg cggactacaa cctgtccaaa
     3361 cgcaccgacg tgtacattca gggcgaatat cagcgggtga ccggtgaccc cacctattcg
     3421 attctggacg atgccttcac gcccggcacc cagtcgccgt catcgacgtc gaagcaggtc
     3481 gtggtgcgcg cggcgatacg gcataagttc tagagatgac tgggtgcgcc agtcgtgtac
     3541 tgaatgatga ccgcgagttc aggcgtgctt gaatgtcgct ttcctgtgtg acgggaaatc
     3601 agttcatcac gtaattctga atcagaatgc cgatgaccac gccggcggga atgccgaagg
     3661 ttctaccgcg aatggtcgcg ctcagatctt cgtcgagtac gaagctgcag cggcgttcac
     3721 gccatacggt tttataggcc aacggctttg catcgacgat aacgagggaa atgagcgcgg
     3781 tcacgatgac ctgttccaaa gcatgcggct tcgccacgaa cggcgaaaat tcaccggcat
     3841 tgcatagcaa tatcatcacg accgcagcga ccagggcgcc gaaaaagaaa ccacccatga
     3901 atctgttcgc tgaattaaga gaatccactt aaacctcaaa tcatccaccg ctttttatcg
     3961 agccggcgaa gtaaaaaata tac
//
LOCUS       DATASH010000979         2408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4719173,
            whole genome shotgun sequence.
ACCESSION   DATASH010000979 DATASH010000000
VERSION     DATASH010000979.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4719173"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VHV99_26110"
     CDS             <1..691
                     /locus_tag="VHV99_26110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HEX3638892.1"
                     /translation="GAVEEQTWSSEKYLDFVPKLFEAVRDKFGFETHLLHDVHHRLTP
                     IEAARLGKSVEPYRLFWLEDPTPAENQAGFRLIREHTVTPIAVGEVFNSIWDCKQLIE
                     EQLIDYIRATLTHAGGITHLRRIADFASLYQVRTGCHGPSDLSPVCMGAALHFDLWVP
                     NFGVQEYMGFPQEALDVFPHAWRFERGMMHPGEAPGHGVDIDEAAAARYPYDPAYLPV
                     ARLEDGTLWNW"
     gene            739..2154
                     /locus_tag="VHV99_26115"
     CDS             739..2154
                     /locus_tag="VHV99_26115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3638893.1"
                     /translation="MNPARPQAAAPASTAMLRRVAFASTIGTAAEYYDFFVYGTAAVL
                     VFGQKFFPSTDPLIGTLAAFATYAVGFLARPLGGIVFGHFGDRVGRKKALIVTILIVG
                     LGTFAIGLLPDYQSIGIWAPVALIVIRVLQGFGVGGEQAGAVLLTAEYAPPVRRGFFA
                     SLVQLGAPAGFLIPSGLFALLSATLTQSQLLDWGWRIPFLASALLVAVGLYIRLRTEE
                     SPIFATMQRTKAVASRPVVEVVRQFGPIIVKGVGAKLIEACAFAMYTMIVLAYGKAHG
                     IAQSTLLETVIVAVALELVTIPLAGALSDRIGRRPTFIAGALLHVALVVPFFMAIDSG
                     NRAAIQLVMILAISVGHSLCYAPQAALFPELFPARVRCSGIALIWQIGSLLGSGILGL
                     LAVKLIQVTHGNSLALIVYVAVLGIVSAVSLFALPETAPLRRGGDLHDWGGGEPVEAH
                     PAAHALSSSSASSSSSFVARH"
     gene            2186..>2408
                     /locus_tag="VHV99_26120"
     CDS             2186..>2408
                     /locus_tag="VHV99_26120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX3638894.1"
                     /translation="MLAAVLHEPKLIRIDEVEPPEPGPGQVRVRVRAGGICGSDLSYY
                     FKGRSGDFAVREPFVLGHEVAGEIDSLGEG"
BASE COUNT          375 a          751 c          778 g          504 t
ORIGIN      
        1 gggcgcggtg gaagagcaaa cctggtcgtc ggaaaagtat ctcgacttcg tgccgaagct
       61 ctttgaagcg gtgcgcgaca agttcggctt cgagacgcat ttgctgcatg acgtccacca
      121 ccgtttgacg ccgatcgaag ccgcgcgcct gggcaaatcg gtcgaaccgt atcgcctgtt
      181 ctggctggaa gaccccacgc ccgcggaaaa ccaggccggt ttccggctga ttcgcgaaca
      241 tacggtcacg ccgatcgcgg tcggcgaagt cttcaacagc atctgggact gcaagcagtt
      301 gatcgaggag caactgatcg actacatccg cgcgaccttg acgcacgcag gcggcattac
      361 gcatttgcgg cgcattgcgg attttgcttc gctgtaccag gtgcgaaccg gttgccacgg
      421 gccgtcggat ctgtcgccgg tgtgcatggg cgcggcgctg cacttcgatt tgtgggtgcc
      481 gaatttcggc gtgcaggagt acatgggttt tccgcaagaa gcgctcgatg tattcccaca
      541 tgcatggcgc ttcgagcgcg gcatgatgca tccgggtgaa gcgccgggcc acggcgtcga
      601 tatcgacgaa gcggcggccg cgcggtatcc ctacgacccc gcatatctac cggtcgcacg
      661 tctcgaagac ggcacgctgt ggaactggtg agctgatttc gctcgaccaa cctcaattac
      721 aaaatcatgg agacacgcgt gaacccagca cgtccgcaag ccgccgcgcc ggccagcacc
      781 gccatgctgc gccgggtggc cttcgcctcg accatcggca ccgcggccga gtactacgac
      841 ttctttgtct atggcacggc cgccgtgctg gtgttcggtc agaagttctt tccctccacg
      901 gatccgctga tcggcacgct ggccgctttc gcgacctacg cggtcggctt tctggcgcga
      961 ccgctgggcg gcatcgtgtt cggtcatttc ggcgatcgcg tcggtcgcaa gaaggctttg
     1021 atcgtgacga tcctgatcgt cgggttgggg acgttcgcga tcggcttgtt gccggactat
     1081 cagtcgatcg gtatctgggc gccggtcgcg ctgatcgtga tccgcgtgct gcaaggcttc
     1141 ggcgtgggcg gcgagcaggc gggcgctgtc ttgctcaccg ccgaatatgc accgcctgtg
     1201 cggcgcggct tcttcgcgag cctcgtgcaa ctgggcgcac cggccggatt cctgattccg
     1261 tcgggactgt tcgcgctgct ctccgcgacg ttgacgcagt cgcaattgct cgactggggc
     1321 tggcgcattc cgtttctcgc cagcgcgctg ctggtcgccg tgggcctgta tatccgcttg
     1381 cggaccgaag agtcgccgat cttcgccacg atgcagcgta ccaaggcagt ggcttcgcgc
     1441 ccggttgtgg aagtggtgcg gcagttcggc ccgatcatcg tcaaaggcgt cggcgccaaa
     1501 ttgatcgagg cttgcgcctt cgcgatgtac acgatgatcg tgctcgccta cggcaaggca
     1561 cacggcatcg cgcaaagcac gctgctggaa acggtgatcg tcgcggtggc gctcgagctc
     1621 gtgacgattc cgctcgccgg cgcgctgtcc gaccggatcg gcaggcgtcc gacctttatc
     1681 gccggtgcgt tgttgcacgt cgcgctggtg gtgccgttct tcatggcgat cgatagcggc
     1741 aatcgcgcag cgatccagct cgtgatgatc ctcgccatta gcgtgggcca cagtctttgc
     1801 tatgcaccgc aagcggcgct gtttcccgaa ctgtttcccg cgcgcgtacg gtgcagcggc
     1861 atcgcgttga tctggcagat tggctcgttg ctcggcagcg gcatactcgg cctgctggcc
     1921 gtcaagctga ttcaggtgac acatggcaat tcgcttgcat tgatcgtcta cgtggccgtg
     1981 ctcgggatcg tgtcggctgt gtcgcttttt gcattgccgg aaaccgcgcc tctgcgccgc
     2041 ggcggcgatc tccatgattg gggcggcggt gagccggtag aagcgcatcc ggcagcgcac
     2101 gcgctctcgt cttcgtctgc atcctcatct tcgtctttcg tcgcgcgtca ctgagcgcgc
     2161 gacctttgcc acgccggagt ccatcatgct tgcagccgta cttcacgaac ccaaactgat
     2221 ccgtatcgac gaagtcgagc cacccgagcc cggccccggc caggtgcgag tccgcgtgcg
     2281 cgccggcggc atttgcggct cggatctgtc gtactacttc aaaggcagga gcggcgattt
     2341 cgcggtgcgc gagccgttcg tgctcggaca cgaagtggcc ggcgagatcg attcgctcgg
     2401 cgagggcg
//
LOCUS       DATASH010000980         2352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3925909,
            whole genome shotgun sequence.
ACCESSION   DATASH010000980 DATASH010000000
VERSION     DATASH010000980.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3925909"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VHV99_26125"
     CDS             complement(<1..438)
                     /locus_tag="VHV99_26125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HEX3638895.1"
                     /translation="MNTVDLTTSPARASEALAAHAASVAAPAAGKRSWLSLIGWIAVL
                     AVLGGAWHGADMRPWDLLSDSGNMGQFAKDFFPPDFTEWRSYLHEMMVTLSVAVWGSA
                     LSLVCAVPCGLMCARNMAPQWVVQPVRRLMDACRAINEMVFAML"
     gene            complement(435..1412)
                     /gene="phnD"
                     /locus_tag="VHV99_26130"
     CDS             complement(435..1412)
                     /gene="phnD"
                     /locus_tag="VHV99_26130"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018423454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX3638896.1"
                     /translation="MKFLRSLITLTVGAAAFACVATAQAEDINLGIISTDSSSVLKER
                     WEPLIADMNKQTGLNVKAFFATDYAGIIEGMRFNKVQVGYFGNASAIEAVDRSQGEVF
                     AKIQYANGNAGYYSVLITNVNSRFKTLDDVFKNTKDVTLGFGDPNSTSGTLIPGYYLF
                     AKHNTPVNTSFKTVLPSSHEANLLAVVNNKIDIATNNTEMLDTLKKEHPDKFAQVRVL
                     WTSPLIPSDPLVWRKDLPQATKDKIRKFFVNYAKTDPHEKAVMAGITGYAGFAASSDE
                     QLVSIRQIALFQQKQKIESDTHLSDNDRKTQLAALDAKLNALNTAVTKQ"
     gene            complement(1455..2351)
                     /gene="phnC"
                     /locus_tag="VHV99_26135"
     CDS             complement(1455..2351)
                     /gene="phnC"
                     /locus_tag="VHV99_26135"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HEX3638897.1"
                     /translation="MRIERLSKTFGNGRKALDEIDLRVEPGEMVALIGASGSGKSTLL
                     RHIAGFTPSDAQPSQIAILGRPIQQNGRIVREVRSIRRDIGFVFQQFNLVNRLSVESN
                     VLIGALARLPLWRRLTGRFPRAERALSLAALNEVGIGDHARERAANLSGGQQQRAALA
                     RALVQRARIVLADEPIASLDPESSRRVMEMLRTLNIEHQLTVLVSLHQVDIAMQYCPR
                     TVALRRGKVVYDGPSTELTPALLKKLYGDDARELLDQAAADSDAAPTASNASGIETGV
                     ATQDTAPTSTRYAFALNPAHSN"
BASE COUNT          426 a          710 c          751 g          465 t
ORIGIN      
        1 cagcatcgcg aagaccattt cattgatcgc gcggcacgca tccatcaggc ggcgcaccgg
       61 ctgcacgacc cactgcggcg ccatgttgcg cgcacacatc agaccgcacg gcaccgcgca
      121 taccagcgag agagccgagc cccacaccgc caccgagagc gtcaccatca tctcgtgcaa
      181 atagctgcgc cattcggtga agtccggcgg gaagaagtcc ttggcgaact ggcccatgtt
      241 gccggagtcg gagagcaggt cccacggccg catatcggcg ccgtgccacg ccccacccaa
      301 cacggcaagc acggcgatcc agccgatcag cgacagccag ctgcgcttgc ccgcggcggg
      361 ggcggcaacg ctcgccgcgt gcgcggcgag cgcttcgctc gcacgcgcgg gcgacgtggt
      421 cagatcaact gtgttcattg cttggtgacg gcggtgttga gtgcgttcag cttcgcatcg
      481 agcgcagcca gctgcgtctt gcggtcgttg tcggaaagat gcgtatcgct ctcgatcttc
      541 tgcttctgct ggaacaacgc gatctggcga atcgacacca actgctcatc cgacgatgcg
      601 gcgaagcccg catagccggt aatcccggcc atcacggcct tttcgtgcgg atcggtcttc
      661 gcatagttca cgaagaattt gcggatcttg tccttggtgg cttgcggcag gtccttgcgc
      721 cacaccagcg ggtcggacgg aatcagcggc gaggtccaca gcacgcgcac ttgcgcgaat
      781 ttgtccggat gctctttttt caacgtgtcg agcatctcgg tgttgttggt cgcgatgtcg
      841 atcttgttgt tcaccacggc cagaaggttc gcttcatggc tcgacggcag caccgtcttg
      901 aacgacgtgt tgaccggcgt gttgtgtttg gcgaacaggt aatagcctgg gatcagcgtg
      961 cccgacgtgg agttcgggtc gccgaaaccg agtgtgacgt ccttcgtgtt cttgaacacg
     1021 tcgtcgagcg tcttgaagcg gctgttcacg ttggtgatca gcaccgagta atagccggca
     1081 ttgccgttcg catactggat cttcgcgaac acttcgcctt gcgagcgatc caccgcttcg
     1141 attgccgacg cattgccgaa atagccgacc tgtaccttgt taaagcgcat gccttcgatg
     1201 atgccggcgt agtcggtcgc gaagaacgct ttcacgttca ggccggtctg cttgttcatg
     1261 tcggcgatca gcggttccca gcgctctttc agcacggagg acgaatcggt cgaaatgatg
     1321 ccgaggttga tgtcctcggc ttgtgcggtg gcgacgcagg cgaatgcggc cgcgccgacg
     1381 gttagcgtga tcaatgaacg caggaatttc atcgctttga gatccggtag agaaaacaga
     1441 atgggtgggc gcgctcagtt cgagtgcgcc ggattgaggg cgaacgcata acgcgtcgag
     1501 gtgggtgcgg tgtcttgcgt cgctacgccg gtttcgatgc cgctggcgtt gctggccgta
     1561 ggagcggcat cgctgtcggc ggctgcctga tcgagcagtt cgcgggcgtc gtcgccatag
     1621 agctttttga gcagggcagg cgtgagctcg gtggaagggc cgtcgtagac caccttgccg
     1681 cgtcgcaacg caaccgtgcg cgggcaatat tgcatcgcga tgtcgacctg gtgcagcgag
     1741 accagcacag tcagttgatg ctcgatgttg agcgtgcgca gcatttccat cacgcggcgc
     1801 gacgattctg gatcgagcga ggcgatcggt tcgtcggcga gcacgatgcg cgcgcgttgc
     1861 accagcgcac gcgccaatgc cgcgcgttgt tgctggccac cggagaggtt ggcggcgcgt
     1921 tcgcgcgcat ggtcgccgat gccgacttca ttgagcgcgg ccaacgacag ggctcgctcg
     1981 gcgcgcggaa agcgtccggt aagacggcgc cacaaaggca gccgcgcgag cgcgccgatc
     2041 agcacattgc tctccacgga aagccggttc accagattga attgctggaa tacgaagccg
     2101 atatcgcggc gaatgctacg cacttcccgc acgatacggc cgttctgctg gatcggccgg
     2161 ccaagaatcg cgatctgcga gggctgcgca tccgagggcg taaagcccgc aatatggcgc
     2221 agcagcgtcg acttgcccga ccccgaggcg ccgatcaacg caaccatttc gcctggctcg
     2281 acacgcagat cgatttcgtc cagcgccttg cggccgttgc cgaacgtctt gctcaaccgt
     2341 tcgatacgaa tt
//
LOCUS       DATASH010000981         2362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_168121,
            whole genome shotgun sequence.
ACCESSION   DATASH010000981 DATASH010000000
VERSION     DATASH010000981.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168121"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /locus_tag="VHV99_26140"
     CDS             <1..1028
                     /locus_tag="VHV99_26140"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX3638898.1"
                     /translation="MGASFVAVNAGKQSVTLNLKDPRGKAILRELVKTADVLVENFRP
                     GVMTRLGLDFDALREVNPKLVYCAISGFGMDGEFSKRPAYDQIIQGISGVMSVTGDAD
                     SAPLRVGYPVSDTVGGLTAAFGICAALVDARASGHGRMLDVSMLESTLATMGWVVSNY
                     LNAGVTPEPMGNENFTAAPSGTFKTGDGPLNIAANENKQFASLCHLIGRADLLDDARF
                     SERNARKVNRAALKAEIEAALASDNATNWEAKFFEAGVPAGRVMSVPEILAHPHLESR
                     EFIREFTGDGSAERQRVTRAGFRLSDGDTAPTTPAPALSAHTREHLASLGYDDAQIDH
                     LRSEGVI"
     gene            1031..1855
                     /locus_tag="VHV99_26145"
     CDS             1031..1855
                     /locus_tag="VHV99_26145"
                     /EC_number="4.1.3.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citryl-CoA lyase"
                     /protein_id="HEX3638899.1"
                     /translation="MTQAFDAAKLAAEYWSTSIIDIHPGSIKVRGFPIQELIGNVSFP
                     QMIWLMLRGELPDAAQARLFEAALVASVDHGPHAPSIAISRMATSCGLPLNGAMASAI
                     NALDDVHGGAGQQAVELYDLIAELMEVGATLEEAVEQGVDAFIAEHGKYLPGFGHRFH
                     PVDPRAVRLLSLVDDSVEQKNISGRYAAIARGIEALLKQRKNKPVPMNIDGATAVIYA
                     ELGFAPELARGVFCLSRAVGILSHAWEQRGRKERNKGPMPRQMNYTYTGKPERHLS"
     gene            complement(1843..>2362)
                     /locus_tag="VHV99_26150"
     CDS             complement(1843..>2362)
                     /locus_tag="VHV99_26150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEX3638900.1"
                     /translation="GALYQRSLNVGDILVPLMRELAETSGESVSFYVRDEAVRVCLHR
                     VDSTHAVRFHVREGDVLPLESGSGGHALLAFSGMPGEPYEKIREDFYCVSIGERDRET
                     AGISMPVFGVRQSVRGVLTLAGPSSRIDQAFFERNLAALFDCAARATDALGGDSRAMR
                     NAQRRAAVVQDR"
BASE COUNT          455 a          746 c          739 g          422 t
ORIGIN      
        1 atatgggggc ttcgttcgtt gcggtgaatg cgggcaagca gtccgtcacg ctcaatctga
       61 aagatccgcg cggcaaggcc attttgcgtg agctcgtgaa aaccgcggac gtgctggtcg
      121 aaaatttccg gcccggtgtg atgacgcggc tcggcctcga tttcgacgcg ttgcgcgagg
      181 tgaatccgaa actcgtgtat tgcgcgatct caggtttcgg catggacggc gagttttcga
      241 aacgccccgc ctacgatcag atcatccagg gtatctcggg tgtgatgagc gtgaccggcg
      301 atgccgacag cgcgccgttg cgcgttggct atccggtgtc ggacacggtg ggcggcctca
      361 cggccgcgtt cggcatctgc gcggcgctgg tcgatgcccg cgcgagcgga catggccgca
      421 tgctcgacgt ttcgatgctg gagtccacgc tggcgacgat gggctgggtc gtgtcgaact
      481 atctgaacgc cggcgtcacg cctgagccga tgggcaacga gaacttcacc gccgcgcctt
      541 cgggtacttt caaaacgggc gacgggccgc tcaatatcgc cgcgaacgag aacaagcagt
      601 tcgcgagtct gtgccatctg atcggccgcg ccgatctgct cgacgacgcg cgtttctccg
      661 agcgcaacgc acgcaaagtg aatcgcgcgg cattgaaggc cgaaatcgaa gcagcgctgg
      721 caagcgacaa cgcgacgaac tgggaagcca aattcttcga agcaggtgtg ccggccgggc
      781 gcgtgatgtc ggtgccggag atcctcgcgc atccgcatct ggaatcgcgt gagttcatcc
      841 gcgaatttac cggcgacggc agcgctgagc ggcaacgcgt gactcgcgcg ggctttcgcc
      901 tctccgacgg tgacacggcg ccgaccacgc ccgcaccggc tttatcggct cacacgcgtg
      961 agcatctcgc ctcgctcggt tatgacgacg cgcaaatcga ccacctacgc tccgaaggag
     1021 tgatctgaac atgacgcaag cattcgacgc cgccaagctc gccgccgagt actggagcac
     1081 ctcgatcatc gatattcatc ccggctcgat caaggtgcgt ggtttcccga tccaggagtt
     1141 gatcggcaac gtcagcttcc cgcaaatgat ctggctgatg ctgcgcggcg aactgcctga
     1201 cgccgcgcaa gcgcgtttgt tcgaagcggc gctggtggcg tcggtggatc atggcccgca
     1261 tgcaccgtcg atcgcaatct cgcggatggc aaccagttgc ggtttgccgc tgaacggcgc
     1321 gatggcttca gcgatcaacg cgctggatga tgtgcatggc ggcgccggtc agcaggcggt
     1381 cgaactgtac gacttgattg ctgaattgat ggaagtgggc gccacgttgg aagaagccgt
     1441 cgagcaaggc gtcgacgcct ttatcgccga acacggcaaa tacttgcccg gcttcggaca
     1501 tcgttttcat ccggtcgatc cgcgggcagt ccggctgctt tcgctcgtgg acgacagcgt
     1561 cgaacagaaa aatatcagcg ggcgatacgc cgctatcgca cgcggcattg aggccttgct
     1621 caagcagcgc aagaacaaac ccgtgccgat gaacatcgac ggtgcaaccg ctgtgatcta
     1681 cgccgagctc ggctttgcgc cggaactggc gcgcggcgtg ttctgcctgt cacgtgcggt
     1741 cggcattctg tcgcatgcat gggagcaacg cggacgcaag gagcgcaaca aaggcccgat
     1801 gccgcgccag atgaactaca cctacacggg taagcccgag cgtcacctgt cctgaacgac
     1861 ggctgcgcgc cgctgcgcat tgcgcatggc gcgcgagtcg ccgcctaacg catccgttgc
     1921 gcgcgcggcg caatcgaaca gcgcggcgag attgcgttcg aagaaagcct gatcgatacg
     1981 cgagcttggg cccgcgagcg tcagcacgcc acgcacactc tggcgcacac cgaataccgg
     2041 catcgagatc ccagccgtct cccgatcgcg ctcgccgatc gatacacaat agaagtcctc
     2101 gcgaatcttc tcgtacggct cacccggcat gccgctgaac gccagcaagg catggccgcc
     2161 ggagccgctt tccagcggca gtacgtcccc ttctcgcacg tgaaaacgca ctgcatgcgt
     2221 cgagtccaca cgatgcaggc acacgcgcac cgcctcgtcg cgcacgtaaa aggacacgct
     2281 ttcgccgctc gtttccgcaa gctcgcgcat cagcggcacc agaatatcgc cgacgttcag
     2341 gctgcgctga tacagcgcgc cg
//
LOCUS       DATASH010000982         6601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1949228,
            whole genome shotgun sequence.
ACCESSION   DATASH010000982 DATASH010000000
VERSION     DATASH010000982.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6601
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1949228"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..796
                     /locus_tag="VHV99_26155"
     CDS             158..796
                     /locus_tag="VHV99_26155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011694426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEX3638901.1"
                     /translation="MKKLLSILFLSLGLVAATAHASPAAPVSGKDYTVLPTPQPTDVP
                     AGKIEVTEFFWYGCPHCNEFNPFLEAWAKKQGPDVVFKRVPVAFRDDFIPHSKMYHAL
                     DALGLAAQLTPKIFNEIHVNKDYLLTPEDQAKFLAKNGVDPKKYMDAYNSFSTQSALQ
                     KDKKLLDDYKIDGVPTLAVQGKYETGPAATDSLPGTIQVLDYLVQQIRAKKM"
     gene            813..1586
                     /locus_tag="VHV99_26160"
     CDS             813..1586
                     /locus_tag="VHV99_26160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3638902.1"
                     /translation="MSSPLKVFITGASSGIGLALAAEYARRGAILGLVARRGDALAAF
                     QQSHPQHSISIYSVDVRDAEALAEAAAQFIAQHGLPDVVIANAGISRGAVTGHGDLRT
                     FREVMDVNYFGMVATFEPFAAALAAAKTGTLVGIASVAGVRGLPGSGAYSASKSAALK
                     YLEALRVEMRPLGVGVVTIAPGYIRTPMTEHNPYTMPFLMDAERFAVKVARAVERQTP
                     FAVFPWQMRIVAALLHVLPRRLYDLAFERAPRKPRVVAE"
     gene            1590..2483
                     /locus_tag="VHV99_26165"
     CDS             1590..2483
                     /locus_tag="VHV99_26165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HEX3638903.1"
                     /translation="MQPRAVDAAGVAHEMAGAHASGVDRRGARGSGVDPLGAPPSGAN
                     VRIVSLVPSITELLFALGLDRQIVGRTGFCVHPRDKVRQVRKVGGTKAVNVDAIRALR
                     PTHLIVNIDENERDTVEQLRAFVPHIVVTHPLTPQDNLSLYSLLGAIFGRVQEAQRLS
                     EALEARLREAAAQAFATQNVLYLIWREPWMTVARDTYISAMLRLVNWQTLPDVEGGPA
                     GAARYPTLDFDHAPWLAGVDRILLSSEPYRFTQAHCDALKRDPRLASKRIELIDGELV
                     SWYGVRAIDGIGYLLGRAVAP"
     gene            2933..4519
                     /locus_tag="VHV99_26170"
     CDS             2933..4519
                     /locus_tag="VHV99_26170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3638904.1"
                     /translation="MRFKLLAAALLFTAPALVLAKPLTVCTESSPDGFDVVQFNSLVT
                     TNASADVIFNSLVSYDESAKKVVPALADKWDVSADGLTYTFHLRPNVQFQTTDYFKPT
                     RALNADDVVFTFDRMLNDSNPWHKVAGASGFPHAQSMGLPKLIKSISKVDDNTVKFEL
                     NAPDATFVSILTMGFASIYSAEYADQLLKAGKQLDLNSKPIGTGPFELKSYTKDAVIR
                     YDVNPTYWGPKPKIDRLIYAITPDATVRAQKVKAGECEIALSPKPQDLAEAKGDKSLA
                     IVQTPAFMTAFVALNTQKKPLDNQKVRAALNMAFDRTTYLKAIFDNTATPAVNPYPPN
                     TWSYDKAVKAWPYDPAKAKKLLADAGYPNGFETTIWVRPNGSVLNPNPKAGAELLQAD
                     FAKIGVKADVKVIEWGELIKEAKQGQHDTLFMGWAGDNGDPDNYLSPLFSCNAVKSGI
                     NFARFCDPDLDKLIADGKASPDQAKRAKAYEQAQQIIHDQALWIPLGYPTAAAIARTN
                     VSGYHVSPFGRQNFGTVAVQ"
     gene            4764..5132
                     /locus_tag="VHV99_26175"
     CDS             4764..5132
                     /locus_tag="VHV99_26175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEX3638905.1"
                     /translation="MSYWDKNAAVSYLQWHAESASQGHCARYVREAIQREGISLMLTY
                     SARNYGSSLICTGFYEVSDANPQKGDVVVIQAIMNHPDGHMAMYDGAIWISDFKQQHG
                     FYPGPAYRSARPSYKIYRHD"
     gene            5142..5606
                     /locus_tag="VHV99_26180"
     CDS             5142..5606
                     /locus_tag="VHV99_26180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3828 domain-containing protein"
                     /protein_id="HEX3638906.1"
                     /translation="MRKSLVLLIATFAVVLSAFFQPFAIAQVHQATTPEASTKAFYTW
                     FIKRDSENRDYALMDKEVYRYVSKPTVDFLRAEYKHNKFAERAEYFTKVQDYDREDWL
                     AHIAPHPMILLDDVALVPVTFGSTEKKTILAFLRKENGIWKITKVDDTQDDK"
     gene            complement(5692..>6601)
                     /locus_tag="VHV99_26185"
     CDS             complement(5692..>6601)
                     /locus_tag="VHV99_26185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX3638907.1"
                     /translation="QGWTVVDCGIASDTIKENWEKVFDSVLDGLPVLRVIVTHCHPDH
                     IGLAHWICSGGDKKRWDVRLWTTLGEYMQARVMAAGDGSNAGGEGAARHFARHGLSDQ
                     ESLEKLRNRTSYYSTLVPALPGQYRRLREADAVKIGGKTWRVVTGFGHSPEHCALHCE
                     ETGTLISGDMVLPRISTNVSVFDMEPEGTPLALYLESLGRYETMPEDTLVLPSHGKPF
                     RGVRTRIKQLREHHDARLAEVREACAAKPQSAADIVPIMFKRQLDIHQMTFAMGEALA
                     HLHLLWLAGELKRTQDADGVIRFSAA"
BASE COUNT         1365 a         2095 c         1929 g         1212 t
ORIGIN      
        1 caacggtgat ttgcagggac gcgccgccac cgaaccatcg gcgtgcggcg cgtgtcacaa
       61 gcacaacgga cgactagagc gtctaacgcc gaatctggaa acggtgacgg gcattgcgtg
      121 gcgtcaccgc tttaccgcct acttggatcg acaacacatg aaaaaactgc tgagcattct
      181 gttcctctcg ctgggcctgg ttgctgccac ggcgcatgca tcgccggccg cgccggtgtc
      241 cggcaaggac tacaccgtgc tgcccacgcc gcaacctacg gacgtgccgg ccggcaagat
      301 cgaagtcacc gaattcttct ggtacggttg cccgcactgc aatgaattca acccgttcct
      361 cgaagcatgg gcgaagaagc aaggtccgga cgtggtgttc aagcgcgtgc cggttgcctt
      421 ccgcgacgac ttcatcccgc actcgaagat gtaccacgcg ctcgacgcac tcggcctcgc
      481 tgctcaactc acgccgaaga tcttcaatga gatccacgtc aacaaggact acctgttgac
      541 gccggaagat caggccaaat tcctcgcgaa gaacggcgtc gatccgaaga aatacatgga
      601 cgcgtacaac tcgttctcca cgcaaagcgc gctgcaaaaa gacaagaagc tgctcgacga
      661 ctacaagatc gacggcgtgc cgacgctggc agtgcagggc aagtacgaaa ccggcccggc
      721 cgcgaccgac agcctgccgg gcacgatcca ggtcctcgac tatctggtgc agcagatccg
      781 cgccaagaag atgtaatagc cgaggcgccg gaatgagttc acccctgaag gttttcatta
      841 ccggcgcctc gagtggcatc ggcctcgcgc ttgccgccga atatgcacgg cgcggcgcga
      901 ttctcggcct ggttgcccgc cgcggcgacg ccctcgccgc tttccagcag tcccatccgc
      961 agcattccat ctccatctat tccgtcgacg tccgcgacgc cgaggcgctc gccgaagcgg
     1021 ccgcgcagtt catcgcgcaa catggtttgc cggacgtcgt gatcgccaat gccggcatca
     1081 gtcgcggcgc ggtcaccggg cacggcgatc tgcgcacctt ccgcgaagta atggacgtca
     1141 actacttcgg catggtcgcc accttcgagc cgttcgccgc cgcgctggcc gccgcgaaga
     1201 caggcacgct ggtcgggatc gccagcgtcg ccggcgtgcg tggcttgccg gggtcgggcg
     1261 cgtacagcgc gtcgaagtcg gccgcgctca agtatctgga agcgttgcgc gtcgagatgc
     1321 ggccgctggg cgtcggcgtg gtcaccatcg cgcccggcta tatccgcacg ccgatgaccg
     1381 agcacaaccc gtacaccatg ccgttcctga tggacgccga gcgcttcgcg gtgaaggtcg
     1441 cccgcgcggt cgaacggcag acgcccttcg ccgtgtttcc gtggcagatg cgcatcgtgg
     1501 cggcgctgct gcatgtgctg ccgcgccggc tctacgacct cgcgttcgaa cgcgcgccgc
     1561 gcaagccccg cgtcgtcgcc gaataaatca tgcagccgcg tgcggtcgat gcagccggcg
     1621 tcgcgcacga aatggcgggc gcccatgcgt cgggcgtcga taggcggggc gctcgtgggt
     1681 cgggcgtcga cccgctcggc gcgcccccct ccggcgccaa tgtccgcatc gtctcgctgg
     1741 tgccaagcat caccgaattg ctgttcgctt tggggctcga caggcagatc gtcgggcgca
     1801 ccggtttttg cgtgcatccg cgcgacaagg tgcggcaggt gcgcaaggtc ggcggcacga
     1861 aagccgtcaa cgtcgacgcg attcgtgctt tgcggcccac ccatctgatc gtcaatatcg
     1921 acgagaacga acgcgatacc gtcgagcagt tgcgcgcgtt tgtgccgcat atcgtcgtca
     1981 cccatccgct aacgccgcag gacaatctct cgctgtactc attgctgggc gccatcttcg
     2041 gccgcgtgca agaggcgcag cgcttgagcg aggcgctcga agcgcgtctg cgcgaagccg
     2101 ccgcgcaggc gtttgccacg caaaacgtgt tgtatctgat ctggcgcgag ccgtggatga
     2161 cagtcgcacg cgatacctac atctcggcga tgttgcggct cgtgaactgg cagacgctac
     2221 ctgacgtgga aggcggaccg gcaggcgccg cgcgctatcc cacactcgac ttcgatcacg
     2281 cgccttggct agccggcgtc gatcgcatac tgctctccag cgagccgtat cgcttcacgc
     2341 aagcgcattg cgacgcgctg aagcgcgatc cgcgtctcgc cagcaagcgc atcgagttga
     2401 tcgatgggga actggtgtcg tggtacggcg tgcgcgcgat tgacggcatc ggttatctgc
     2461 tcgggcgcgc ggtcgcacca tgagcgctgg cgcgaagccc tggccaccga gtagcgccgc
     2521 ctgacgctcg catgagccgc gtcactgccg cacgccgcct ggagacgacc ctgggcgaag
     2581 ccactgccac acgccacgtg gagacgaccc tgggcgaagc cactgccgca cgccccgtgg
     2641 agacgaccct gggcgaagcc actgcgcgag gccgtgcccc gcacaccgac tcccgctgcc
     2701 catatggata tgcggattaa tttgttgtcg ccgcaaccac ccttcgataa gctttcgcga
     2761 aggccgcgac gcgcatgggc tatagcagcc acaccatgcg cccgcgccgg aaacgtatca
     2821 caataaagac gacggccaga ccggccaccg atcaccggtg cgttcgcccg tcaaccgaat
     2881 acaaccacac caccagacgc agtgcctgac tgcgcttgtt atgggagatc ctatgcgctt
     2941 caaactgctc gcagccgcct tactgttcac ggcgcccgcg ctcgtgctcg ccaagccgct
     3001 gaccgtctgc accgagtcga gccccgacgg ctttgatgtc gtgcagttca attcgctcgt
     3061 gacgaccaat gcgtcggccg acgtgatctt caactcgctg gtctcgtacg acgagtccgc
     3121 gaagaaagtc gtgcccgcgt tggccgacaa gtgggacgtg agcgccgacg gcctcaccta
     3181 cacgttccat ctgcgcccga acgtgcagtt ccagactaca gactacttca aacccactcg
     3241 cgcgctgaat gccgacgacg tcgtcttcac attcgaccgc atgctcaacg acagcaatcc
     3301 gtggcacaag gtcgcgggcg cgagcggctt cccgcatgcg caatcgatgg gcttgccgaa
     3361 gctgatcaag tcgatcagca aggtcgacga caacaccgtc aagttcgaac tcaacgcgcc
     3421 ggacgcgacc ttcgtgtcga tcctgacaat gggtttcgcc tcgatctatt cggctgaata
     3481 cgccgatcag ttgctcaagg ccggcaagca actcgacctg aattcgaagc cgatcggcac
     3541 cggtccgttc gaattgaaga gctacaccaa agacgcggta atccgctacg acgtgaaccc
     3601 cacgtactgg ggcccgaaac cgaagatcga ccgcctgatc tacgcaatca cgccggacgc
     3661 caccgtgcgc gcgcaaaagg tgaaggccgg cgaatgcgag atcgcgttgt cgccgaagcc
     3721 gcaagatctc gccgaagcga agggcgacaa atcactcgcc atcgtgcaga cgcccgcgtt
     3781 catgactgcg ttcgtcgcgt tgaacacaca gaagaagccg ctcgacaacc agaaggtgcg
     3841 cgccgcgctg aacatggcgt tcgaccgcac cacgtacctc aaggcgatct tcgacaacac
     3901 cgcgacaccg gcggtcaatc cgtatccgcc gaacacgtgg agctacgaca aggccgtgaa
     3961 ggcgtggccg tacgatccgg cgaaggcgaa gaagctgctc gcggacgcgg gctatccgaa
     4021 cggcttcgag acgacgatct gggtgcgtcc gaacggcagc gtgctgaacc cgaatccgaa
     4081 agccggcgcc gaactgctgc aagccgactt cgcgaagatc ggcgtgaagg ctgatgtgaa
     4141 ggtgatcgaa tggggcgagc tgatcaagga ggccaaacaa ggccagcatg acacgctgtt
     4201 catgggctgg gccggcgaca acggtgatcc ggataactat ctgtcgccgc tcttcagttg
     4261 caatgcagtg aagtcaggga tcaatttcgc gcgcttctgc gatccggatc tggacaagct
     4321 gatcgccgac ggcaaggcat cgccggatca ggccaaacgc gcgaaggcgt atgaacaggc
     4381 gcagcagatc atccacgatc aggcgctttg gattccgctg ggttatccga ctgcggcggc
     4441 gatcgcgcgc acgaacgtga gcggctatca cgtgagtccg tttggacggc agaactttgg
     4501 gacggtggcg gtgcagtaat cgcgcctgcg tctggatgta cgttaaaaag cccggttcga
     4561 aagcaccggg ctttttttgt cgcgagcatc gcgatattgc tctcggattc aagcgataca
     4621 gtatgcattg caggtatctc gataacgaat attttcagag tttactttaa tcatcgaaaa
     4681 ggttaaacag ttgttaaatt ttcaacgcgg ccccgataaa taacgctagg atgtttttcc
     4741 aatttacttt ggatacctat tttatgtctt attgggacaa gaacgccgct gttagctatc
     4801 ttcagtggca tgccgaatct gcaagtcaag ggcattgtgc aaggtacgtg cgagaggcga
     4861 tccagcgcga gggaatttca cttatgctca cgtactccgc gaggaactac ggctcctcat
     4921 taatctgcac gggcttttac gaggtaagcg acgcaaatcc gcagaaaggg gatgttgtcg
     4981 tgatccaggc cattatgaat catcccgatg ggcatatggc aatgtacgac ggtgcgatct
     5041 ggatttccga cttcaaacaa cagcacggct tctaccccgg tcctgcgtat agaagcgcgc
     5101 gtccttccta taagatctat cgtcatgact gagcgcaaat catgcggaaa tccctagttc
     5161 ttcttattgc gaccttcgct gttgttctaa gcgccttttt ccaaccgttt gcgatcgcgc
     5221 aagtgcacca ggcgacgacg ccagaagcca gcacaaaggc gttctacaca tggttcataa
     5281 aacgggactc tgaaaaccgc gactatgccc tcatggacaa agaggtctat cgctatgttt
     5341 caaagccaac cgtcgacttc ctgagggccg aatataagca caacaagttt gctgagaggg
     5401 ccgagtactt tacaaaggta caggattacg accgggaaga ttggctcgcg catattgcgc
     5461 cgcatccgat gatcctgctt gatgatgttg cgcttgttcc cgttacgttc ggatcgacgg
     5521 agaagaaaac cattcttgca tttctacgca aagagaatgg catctggaag atcaccaagg
     5581 tagacgacac gcaggatgac aagtagcgtt tgggaacacc gcctgatcaa cgtggcgtct
     5641 gccaattaaa gcccgcgtcc gcgggcctaa ttcatacaca caccaccgca atcaagccgc
     5701 cgaaaaccgg atcaccccat ccgcgtcctg cgtgcgcttc aactcgcccg cgagccacag
     5761 caaatgcaaa tgcgccaacg cctcgcccat cgcgaacgtc atctgatgga tgtcgagctg
     5821 gcgtttgaac atgatcggca cgatatccgc ggcgctctgc ggcttcgccg cgcaggcttc
     5881 gcgcacttca gccagacgcg catcatgatg ctcacgcagt tgcttgatac gcgtacgcac
     5941 gccgcgaaac ggcttgccgt gtgacggcag cacgagcgtg tcttcgggca tcgtttcgta
     6001 gcggccgagc gattccagat acagcgcgag cggcgtgcct tccggctcca tgtcgaacac
     6061 cgacacatta gtcgagatgc gcggcagcac catgtcgccg gagatcagcg tgccggtctc
     6121 ttcgcaatgc aacgcgcagt gttccggcga atggccgaag ccggtcacca cgcgccacgt
     6181 cttgccgccg atcttcacag catcggcttc gcgcagacgg cggtactgcc cgggaagcgc
     6241 cggcacgagg gtcgagtagt agctcgtgcg attgcgcagc ttctcgagcg actcctgatc
     6301 gctcaaacca tgacgcgcga agtgccgcgc ggcgccctcg ccgcccgcat tcgaaccgtc
     6361 gccggccgcc atcacgcggg cctgcatgta ttcgccgagc gtcgtccaca aacgcacgtc
     6421 ccagcgtttc ttgtcgccac cagagcaaat ccagtgcgcg aggccgatat ggtccggatg
     6481 gcaatgcgtc acgatcacgc gcagcaccgg caagccgtcg agcacagagt cgaacacttt
     6541 ctcccagttc tccttgatcg tgtccgaggc aatgccgcaa tcgactaccg tccagccttg
     6601 c
//
LOCUS       DATASH010000983         3309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2739822,
            whole genome shotgun sequence.
ACCESSION   DATASH010000983 DATASH010000000
VERSION     DATASH010000983.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2739822"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="VHV99_26190"
     CDS             complement(<1..675)
                     /locus_tag="VHV99_26190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3638908.1"
                     /translation="MVEAFEVTKRFGSTPALKDVSIRVMPGESHALVGRNGAGKSTLV
                     SILTGLRKPDTGEVRFGGVAAPSIADRDAWRERVACVYQHSTIIRDLSVAENLFINRQ
                     PARGGVIDWRAMRRDARKLLDHWKIDVREDARAADLSVEARQLVEIARALSYGARFII
                     LDEPTAQLDGDEIKRLFRRISELQREGVTFLFISHHLQEVYEICQAVTVLRDARHIVS
                     APVCALP"
     gene            complement(777..1826)
                     /locus_tag="VHV99_26195"
     CDS             complement(777..1826)
                     /locus_tag="VHV99_26195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX3638909.1"
                     /translation="MSFAKGPHAARRKFRSSLFAVAAAACVAGLAVNGAARAADAGKI
                     GLDLPLLTSPFWQSYNNYLPKYAKDMGMDILAPVNSNGDPVQQITDMNNLLNLGSKGI
                     VVGPLDSAAISRALDAAAAKNVPVVAVDVAPTQGKVAMVVRADNRAYGEKACKYIGEH
                     VKSGKVVQIMGDLASVNGRDRSEAFRSCLKGYPGLSLLEIPASWKGDVAATALDSLLT
                     ANPDVKAIYMQAGGVYLSPTLQTLRRKQMLFPAGDAKHVVIVSNDGIPQEFEAIRRGD
                     IDATVSQPADSYAKYGLFYIKAALAGQTFKPGPTDHGSNIVQLSPGVLEDQLPAPLVT
                     KSNVDDKALWGNTVK"
     gene            2244..3038
                     /locus_tag="VHV99_26200"
     CDS             2244..3038
                     /locus_tag="VHV99_26200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX3638910.1"
                     /translation="MDADDKDDADRYRAPALDKGLDILELLAEQKEGLTRAEITKLLG
                     RNASEMYRMLERLVARQYVVRSAAGDRYSLSLKLYALAHRHPPMNRLISEALPLMQRF
                     ADAAEQSCHLVVYDRGNLLVIAQVDGPGTWGMSVRLGSRVGLIDTGSGRVMLAFQSVE
                     QREQMLAEHTKVKGEVTIDRDALEQACARIRAAGFSQKDSQQTFGVTDVTFPIQGPAG
                     QAIAVLTCPYLRRIDEYVAPTLEAATALLRETVQALSMFREQSRST"
BASE COUNT          625 a         1013 c         1024 g          647 t
ORIGIN      
        1 cggcaacgcg caaaccggcg cgctgacgat atgccgcgcg tcgcgcagca ccgtcacggc
       61 ctggcagatt tcatagacct cttgcagatg atgcgagata aacaggaacg tcaccccttc
      121 gcgctgcaac tcgctgatgc ggcggaacag gcgcttgatc tcgtcgccgt cgagctgcgc
      181 ggtgggttcg tcgaggatga tgaagcgcgc gccatacgac aacgcccgcg caatctccac
      241 cagttggcgc gcttccacgc tcagatcggc ggcgcgtgcg tcttcacgta cgtcgatctt
      301 ccagtggtcg agcaacttac gcgcgtcgcg gcgcatcgcg cgccaatcga tcacaccgcc
      361 gcgtgccggc tggcggttga tgaacagatt ctctgcaact gagagatcgc ggatgatcgt
      421 cgagtgctga taaacgcagg caacgcgttc acgccatgcg tcgcggtccg cgatcgaggg
      481 cgcagccacg ccgccgaaac gcacttcacc ggtatcgggc ttgcgcaggc cggtcaggat
      541 cgacaccagc gtcgatttgc ccgcaccgtt gcgcccgacg agcgcatgcg actcgcctgg
      601 catcacgcgg atgctcacgt ctttcagcgc aggcgtggag ccgaagcgtt tggtgacttc
      661 aaacgcttcg accaccgcgt gaagatcctg cgcgccgaag gcagcccccg tgggggatgc
      721 cccgaaggaa actccctcgg gggatgggac caacggaacg gagggcgtcg gctcgctcat
      781 ttaacggtat tgccccacaa cgctttgtcg tcgacattcg acttcgtcac gagcggtgcg
      841 ggcaattgat cttcgagtac gcccggcgac agctggacga tgttgctgcc atgatcggtc
      901 gggcccggct tgaaggtttg cccggccaac gcggccttga tgtagaagag gccgtatttc
      961 gcatacgaat cggcgggctg cgacacggtg gcgtcgatgt caccgcgacg gattgcctcg
     1021 aactcctgag gaatgccgtc gttactgacg atcacgacat gctttgcatc gccggcggga
     1081 aacagcatct gcttgcggcg cagggtttgc agcgtcggcg acagatagac gccgcccgcc
     1141 tgcatgtaga tcgccttcac gtcgggattc gcggtgagca gactgtcgag cgcggtggcg
     1201 gcgacatcgc ctttccagct tgccggaatc tcgagcagcg acaaacccgg ataacccttc
     1261 aaacacgagc ggaacgcttc ggaccggtcg cgcccattca ccgacgccag gtcgcccatg
     1321 atctgcacaa ccttgcccga cttcacatgc tcgccgatgt acttgcacgc tttctcgccg
     1381 taggcgcgat tgtcggcgcg taccaccatc gccaccttgc cttgcgtcgg cgcgacgtcg
     1441 accgcgacca ccggcacgtt cttcgccgct gcggcgtcga gcgcgcggct gatcgccgcc
     1501 gaatccaacg ggccgaccac gatgcccttc gagccgagat tcagcaggtt gttcatgtcg
     1561 gtgatctgct gaacgggatc gccgttcgaa ttgaccggcg cgaggatatc catgcccatg
     1621 tccttcgcgt acttgggcag atagttgttg tacgactgcc agaacggcga cgtgagcaac
     1681 ggcagatcga gaccgatctt gccggcgtcg gcggctcgtg cggcgccatt aacggcgagg
     1741 ccggcgacgc aagctgccgc cgcgacggca aacagtgaac tgcggaactt acggcgagcc
     1801 gcgtgcggcc ctttcgcgaa cgacatggtt tgtctccttc tcctgtgtcg gctggagtta
     1861 gcgctttagc gcttactcca gccctatgca ccgctgtcgg ctggagttag cgctttagcg
     1921 cttacttcag ccccatgcac cgctgtcggc tggagctagc ggtttagcgc ttactccggt
     1981 cccatgcacg gtagtgcggt atggaactac gcaacccgcc gcgtgatggc gagcacttcg
     2041 tcctgcgctt tcttatgaag tattggtgtg tattgattca ccaatgaacg tattattcat
     2101 atacggactt ttcgaagtca aggaacgtgc actgcaccat gcgtcggtgt ttgccctgga
     2161 ctcgcgacac ttcgcatgag aatcaccccg cacttacact cacgcgccag ataatgagaa
     2221 aagcacacgg agccttgctc gccatggacg cagacgataa agacgacgcc gaccgttacc
     2281 gcgcgcccgc gctagacaag ggtctcgaca ttctcgaact gctcgccgag cagaaagaag
     2341 gactgacgcg cgccgaaatc accaagctgc tgggacgcaa tgcgagcgag atgtaccgga
     2401 tgctggagcg actggtcgcg cgacagtacg tagtgcgctc ggcggcgggc gaccgttatt
     2461 cgttgagcct gaagctatat gcgctcgcgc atcgccatcc gccgatgaac cggctgatct
     2521 ccgaagcgct gccgctgatg cagcgctttg ccgatgctgc cgagcaatcg tgtcatctcg
     2581 tggtgtacga ccgcggcaat ctgctggtga tcgcgcaggt ggatggaccg ggcacgtggg
     2641 ggatgtcggt acggcttggt tcgcgggtcg gcctgatcga taccggttcg ggccgcgtga
     2701 tgctcgcgtt tcaaagcgtc gagcagcgcg aacagatgct tgccgaacac accaaggtga
     2761 agggcgaagt cacgatcgat cgcgatgccc tggaacaggc atgcgcgcgg attcgcgcgg
     2821 cgggtttttc gcaaaaagac agtcagcaga ctttcggcgt gaccgacgtt acgtttccga
     2881 ttcaggggcc ggccggtcag gcgattgcgg tgctcacctg cccgtacttg cgacgcatcg
     2941 acgaatatgt cgcgccgact ctggaggcgg cgactgcgct gttgcgcgag acggtgcagg
     3001 cgttgtcgat gtttcgggag cagagccgct caacctgacg caccttgggg taacggcaaa
     3061 tgggtgcttc aatgcgtggc cggcgcgccg tcattcaggg ctcggctctc gcatgaagct
     3121 atgtcaacgc gacctataaa gtgttgccat aggctctgtc aaaagttgac ataagcgccg
     3181 tcgagcgcct caccgttgtt atcgttggcg cgaagcgatc gcgagttgat gtcatctctt
     3241 ccgaagcgcc tcgcctggca catggtttag aatgacgacc ctctcaaaaa tcgcgccgta
     3301 tgggatacc
//
LOCUS       DATASH010000984        13835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2542462,
            whole genome shotgun sequence.
ACCESSION   DATASH010000984 DATASH010000000
VERSION     DATASH010000984.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 13835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13835
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2542462"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(218..1246)
                     /locus_tag="VHV99_26205"
     CDS             complement(218..1246)
                     /locus_tag="VHV99_26205"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013337766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEX3638911.1"
                     /translation="MNDDILDPQDGADSAVPFARLKPEIVLDALDSVLSSVGVYTDGR
                     MLPLNSYENRVYQVGVEDGPPVVAKFYRPERWTDAAILEEHAFVADLAAREIPAVPAR
                     VFEGRTLHTFDGFRFSIFERRGGRAPDLDRRDTLEWLGRFIGRIHAVGQTQNYVERPT
                     LDINTFGYEPRDFLLSHRFVPDDVRTAWKTVVNLALEGVERAFERAGEIRMLRMHGDC
                     HPSNVLWTDAGPHFVDFDDSRMGPAVQDLWLLLPGERAEASRALADLLAGYEDFCDFE
                     PRELYLVEALRTLRLIHYQAWLARRWDDPAFPAAFPWFNTQRYWEDRILEMREQLGAM
                     EEGPLWPV"
     gene            complement(1349..1561)
                     /locus_tag="VHV99_26210"
     CDS             complement(1349..1561)
                     /locus_tag="VHV99_26210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638912.1"
                     /translation="MQIGSIVRSVHIAVPQGARGIVMRILGDMAMVAWYAGEPGTSEH
                     LNTEPFFLEDLIDTGEQVRPSSAQVH"
     gene            1978..5160
                     /locus_tag="VHV99_26215"
     CDS             1978..5160
                     /locus_tag="VHV99_26215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX3638913.1"
                     /translation="MWIVRLALRRPYTFVVLALLLLIVGPLTILRTPTDIFPNIDIPV
                     LSVIWSYNGLPADEMEKRIVLNYERGLSTAVNDIEHTESTSLNGIAVVKIFFQPHANI
                     DEALAEVTALSQTQLRSLPPGITPPNILRYNASTVPILRLALSSASLTEQELFDFGNN
                     FLKTQLATVPGASAPLPYGGKQRQIMVDIDSRKLQERNLSPMDVVNAVTAQNLILPSG
                     TAKIGSTEYSVEMNGSPDSLAGLNNIPIKSTANGTVYIRDVAHVRDGFQPQTNIVRVN
                     GQRAALLTINKSGNTSTLEIVDRIKKMMPTLRNLVPASLNIDPVADQSLFVRASVQGV
                     LREALIAACLTGLMILLFLGNWRATLIIAVSIPLSMITSIIALSMLGETINIMTLGGL
                     ALAVGILVDDATVAIENISHQLEQGKTLEQAILDGAHQIAIPTLVSTLSICIVFVPMF
                     LLTGVAHYLFIPLAEAVVFAMLASYFFSRTLVPTLAKYLLRHHHKPADLHHAPPQTRN
                     PFMRVHYAFEGGFARFRDRYREFLEARVARPGLFVTLFLVCCGASMLLMPFLGRDFFP
                     AVDAGTIALHLRAKTGMRVEETAVVTDRVDTRIRQLIPPAELHSLIDNIGLPVSGINL
                     SYSNTGTIGTSDSDILITLNPDHHPSADYVRTLRRTLTDEFPGVQFAFLPADIVSQTL
                     NFGMPSPIDIQIVGRDVAGNRVFAAQLLNRLRTVPGLADARIQQPADLPRIFIDVDRT
                     RAQQAGFSQRDIASNLLITLSGSQQTTPTFWLNPRNGVSYNVITEAPQYTIDSLQSLA
                     NIPLNANGRSNILGSLATMKREAGNAVLTHYNAQTTIDIFGTADGRDLGGVSDDIRKI
                     IDDSKANLPKGSSIEVRGQVQTMNDSFSGLFAGLVFAILLVYLLIVVNFQSWLDPFII
                     ITALPGALAGIVWMLFLTHTTLSIPALTGAIMCIGIATANSILVISFAREQLLEHGDA
                     TRAAIEAGFTRFRPVLMTALAMVIGMVPMAIGLGEGGEQNAPLGRAVIGGLTVGTLAT
                     LIFVPVVFSMIYRRLAARRQRAAEHVVQKP"
     gene            5237..6613
                     /locus_tag="VHV99_26220"
     CDS             5237..6613
                     /locus_tag="VHV99_26220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX3638914.1"
                     /translation="MEGKRPDGSNSALNPAKQKRARWVIVAVAVVVAVLAAQGIWSRH
                     DAHAALERDAEHASQTSVEVVRPQKSTVGLDLVLPGNVQAFLDTPIYARTNGYLKKWY
                     ADIGAKVKNGQLLAEIDTPEVDDQLRAARADLANADANYALAKSTADRWTEMLKSKSV
                     SKQETDEKVGDMLAKKGTLDAARFNVARLEKTQSFQKVYAPFDGIVTARNVDVGALID
                     AGSSGGPAKELFHVAQADRLRVYVNVPQAYALQVRAQQTAFLTLTETPSKHYPGTVAR
                     TAGAVDPQQRTMLVEVDVDNRNGDLLPGAYAQVHFALGAGAAPFTLPGNALLFRPDGV
                     KVATVDPQHKVKLLPVSLGTDFGTRVAVASGLQGNEQVILNPQDSIVDGAPVRIVPGK
                     AGAGAGGVSSVEGAPGASGAAAAGEGAAGASGAAAAGEGTTGASGAAATGVPAAHVAG
                     LQAVRNAL"
     gene            6610..8118
                     /locus_tag="VHV99_26225"
     CDS             6610..8118
                     /locus_tag="VHV99_26225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEX3638915.1"
                     /translation="MSAPALPRRFAPRLTRVALLGAFACSLTACTVGPDYVKPPAATA
                     AAYKELEGTGWKPAQPADTLTRGAWWSVYADPSLDALEQQVASANQNVQAAEARFRAA
                     RATVAQYRSNFFPIVSANGDYSRTRTSENVLHKSTAGLTLNDYLVQADASWEPDLWGR
                     VSRSVEGAKAEAQASAADAQAVLLSMQAELATDYFELRGFDQERQLLDDTIKAYKEAL
                     DLTQHRYAGGIATDADVAQAQTQLQTTQAQALDLGVQRSQLEHAIAILIGQTPSTFSL
                     PVAPLTVVPVVAAAGVPSALLERRPDVASAERHVADMNAQIGVATAAFFPSLILSVTG
                     GLEATNYSQWLLAPSRLWSLGPTLAGTLLDFGGRASVKEQARAHYDESVAQYRQTVLT
                     AFGQVEDNLAALRVLEQEAAAQDDAVAAAQRSLAVVSNRYKNGAITYLDVVVAQTTAL
                     TNERDAVSIARRRMAASVALVKALGGGWDASALPTDEQIVHPTAPASEAAHG"
     gene            8300..9589
                     /locus_tag="VHV99_26230"
     CDS             8300..9589
                     /locus_tag="VHV99_26230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3638916.1"
                     /translation="MTAPPESHRTATSAVPAPPATSPSAAVPDVAHTPYLERGTRAYW
                     RASIALLFAGYATFSLLYCVQPLLPAFSKAFSVTPAQSSLSLSVTTAALALAIFVAGF
                     VSEGWSRHKLMTASLTLSALLTIGVSIAPQWHQLLVLRALEGLALGGVPAVAMAYLAE
                     EVHPDGLGLAMGLYVGGTAIGGMAGRVITGVVADLFSWRVAIGTIGVLGILSMLAFRA
                     LLPPSRHFVPRRGLGFAHHHAALLKQFTRPGLPLLFLLGFVLMGSFVTLYNYIGYRLL
                     APPYLLSQTEIGAIFVVYLTGVIASPWSGRMADTFGRARVLIGSLLLMALGLALTLLH
                     PIAAIAIGIACLTFGFFAGHSVASGWVGRLAKEAKGQAAALYLLAYYIGSSVVGSYGG
                     HVWAAYGWNGVAGLVGVLLVVGIVAAVRLRQREETAG"
     gene            9939..10124
                     /locus_tag="VHV99_26235"
     CDS             9939..10124
                     /locus_tag="VHV99_26235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010100910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638917.1"
                     /translation="MTTYFTIGDFILVIPMALAGALFVGALPCKTELRHNALRVLGAL
                     IGVVFAVVLVEGLPALV"
     gene            complement(10315..11358)
                     /gene="mgrA"
                     /locus_tag="VHV99_26240"
     CDS             complement(10315..11358)
                     /gene="mgrA"
                     /locus_tag="VHV99_26240"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HEX3638918.1"
                     /translation="MAYEAASERYSDMQYRVCGKSGLKLPALSLGLWHNFGDTTPIST
                     QRDILRTAFDLGITHFDLANNYGPPYGSAETNFGRLFKDDFKPYRDELLISSKAGWDM
                     WPGPYGQGGGSRKYVLASLDQSLQRMGLDYVDIFYSHRFDADTPLEETAGALASAVQQ
                     GKALYIGISSYSATKTLEMARLLAEYKVPLLIHQPAYNMLNRWIERELLDALEKAGAG
                     AIAFTPLAQGLLTGKYLNGVPEDARVNKPGGGSLKQEHLSPQNIEHVRKLNDIAQRRG
                     QSLAQMALAWALRDPRVTSVLIGASRAEQVRENVGALKNLAFSKEELAEIDRYATEGG
                     VNLWEKPSTDQAI"
     gene            11580..11786
                     /locus_tag="VHV99_26245"
     CDS             11580..11786
                     /locus_tag="VHV99_26245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3185 family protein"
                     /protein_id="HEX3638919.1"
                     /translation="MTKAISIALVVGGIVLLYFGGQAFNSVSSEVSRVFTGSPTNKAI
                     MLLVGGVIATIAGLTGMAVSGRKR"
     gene            complement(11847..12608)
                     /locus_tag="VHV99_26250"
     CDS             complement(11847..12608)
                     /locus_tag="VHV99_26250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX3638920.1"
                     /translation="MSLHSKKEQIHTLREQGFVVVPGLVSPERCGELKQIAERQLQEA
                     AAPIEFEADLQYPGAPESKHAPGGHTVRRLLDAYGRHPSFAQWATAPEIRGWMELYFG
                     EEPRLSRAHHNCMMTKHPAYGSLTGWHRDVRYWSFERDDLVSVWVAVGSETVDNGGLW
                     FVPKSHSAAFTSERFDEAKFFRSDLEENQALIRTAVSPELKAGDVVFFHCNTLHSAGK
                     NLSDQVKFSLVFTYHGASNVPLPGSRSAGKPEIAF"
     gene            13070..13438
                     /locus_tag="VHV99_26255"
     CDS             13070..13438
                     /locus_tag="VHV99_26255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638921.1"
                     /translation="MHTLLSTRLTRAALKAARPARRHLVRALRHHATRTRALGEQWRE
                     AKAVDGIRTWFHTFFSALRLRGSSRRFSLRTLLRKPTPLRLTAKQTAPVPAPQSTSRR
                     PRRMSTSGSTGWFAFAFASR"
BASE COUNT         2554 a         4417 c         4212 g         2652 t
ORIGIN      
        1 ttgagaatcc agcacccaga aacagtttct cgcttgactt ccaatacggt tgcttttctt
       61 tgcggcaggc aaagaaaagt aggtgcccgc cccgcacagg ggcgacgcta atagaccaat
      121 atcaattcaa ggaaaggcca acgccgcagg caaacagaac cacgaacgag caaccacccc
      181 aaatcaaaaa accccaacac cctacaaccg caaacactca aaccggccaa agcggccctt
      241 cctccatcgc accgagctgc tcacgcattt cgaggatgcg gtcttcccaa tagcgctgcg
      301 tgttgaacca cggaaacgcc gccggaaagg ccgggtcatc ccaacggcgc gcgagccacg
      361 cctggtaatg aatcagccgc aacgtgcgca acgcttcgac gagatacagt tcgcgcggct
      421 cgaagtcgca gaagtcttcg taaccggcga gcagatccgc cagcgcacgt gacgcctcgg
      481 cgcgctcgcc cggcaacagc agccacaaat cctgcaccgc cggacccatg cggctgtcgt
      541 cgaagtcgac gaaatgcggg cccgcgtcgg tccacaacac attgctcgga tggcaatcgc
      601 catgcatccg cagcatgcga atctcgccgg cacgctcgaa ggcgcgttcg acgccttcaa
      661 gcgcgagatt caccacggtc ttccacgccg tccgcacgtc gtcgggcacg aagcggtgcg
      721 acagcaggaa gtcgcgcggc tcgtagccaa acgtgttgat gtcgagtgtg ggacgctcga
      781 catagttctg cgtctggccc accgcatgga tacggccaat aaatcgcccc agccattcga
      841 gcgtatcgcg ccggtcgaga tcaggcgcgc ggccgccgcg gcgctcgaaa atcgaaaagc
      901 ggaacccgtc gaaggtatgc aacgtgcggc cttcgaaaac acgcgccggc accgccggta
      961 tctcacgcgc ggcgagatcg gcgacaaagg catgttcttc aagaatggcg gcgtcggtcc
     1021 aacgctcggg acggtagaac ttggcgacca ccggcggacc gtcttccacg ccgacctggt
     1081 acacgcggtt ctcgtagctg ttgagcggca gcatgcgtcc gtcggtgtac acgccgacgc
     1141 tgctgagcac gctgtcgagc gcgtcgagca cgatttccgg cttgagccgg gcgaaaggca
     1201 cggcgctatc ggcgccgtcc tgtgggtcga gaatgtcgtc gttcatgccc gcattgtgcg
     1261 ccgcgccgcc gtcaaagacg agggcgccgg ctcaccttgc ggcttacagt caccttcaat
     1321 agccatgaaa cggactcaca aatcagtttc aatgcacctg cgcgctcgac ggacgcacct
     1381 gctcgcctgt atcaatcaga tcttcaagga aaaagggttc agtgttgaga tgctccgaag
     1441 tgccgggctc gccggcatac caggccacca tggccatgtc gccgagaatg cgcatgacaa
     1501 tcccgcgcgc gccttgcggc acggcgatat gcaccgatcg gacaatggaa ccgatttgca
     1561 tgatgtttcc ccgtttctcc catagccggc tttatgattt ccgcgccggt caaggtgatg
     1621 ataaaagcgc cgcttcgccg ataacgttgc gtacgcaccc aatctgctgc gcgtatgacg
     1681 cgtgaaaggt tcgagtcatt gttcccgtgt tgcggggcca tgaaaggggc gaactcaaga
     1741 tcgtcgaccc gtcgtttcgc cctattccca ttacaacgcg ctgggccgat gatctgggca
     1801 taaagcgtcg taacgagacc atcataaacc cagccggggc gatatatcaa aatcatggac
     1861 cgccagaaca cccccattaa tccagtcatc gtgctcaccg tccgccgccg tggcatgctc
     1921 tttagccgct gcgcgcgcca cgccggcggc ttcggcgttc atcgaccgca ctgacccatg
     1981 tggattgtcc gtctggcgct gcggcgcccc tatacgtttg tcgtgctggc cctgcttttg
     2041 ctgatcgtcg gtccgttgac gattttgcgc acacccaccg acatcttccc caatatcgac
     2101 attccggtgc tctcggtgat ctggtcgtac aacggactgc cggccgacga gatggaaaag
     2161 cgcatcgtgc tcaactacga gcgcgggctg tcgaccgcgg tcaacgatat cgagcacacg
     2221 gagtcgactt cgctgaacgg gatcgcggtg gtaaagatct tttttcagcc tcacgcgaac
     2281 atcgacgaag cgctcgccga agtcacggcg ctgtcgcaga cgcaattgcg ctcgttgccg
     2341 cccggcatca cgccgccgaa tatcctgcgc tacaacgcct cgacggtgcc gatcctgcgc
     2401 ctcgcgctgt cgtccgcttc actcaccgag caggagctgt tcgacttcgg caataacttt
     2461 ctgaagacgc agctcgccac cgtgccgggc gcatcggcgc cgctgccgta tggcggcaag
     2521 cagcggcaga tcatggtcga tatcgattcg cgcaagctgc aggagcgcaa tctctcgccg
     2581 atggacgtgg tcaacgccgt caccgcgcag aatctgatcc tgccttcggg tacggcgaag
     2641 atcggctcga cggaatattc ggtggagatg aacggcagtc cggattcgct ggcgggcctc
     2701 aataacattc cgatcaaatc caccgccaac ggcaccgttt atatccgcga cgtcgctcac
     2761 gtacgcgacg gtttccagcc gcagaccaac atcgtgcgcg tgaacggcca gcgtgccgcg
     2821 ttgctgacca ttaataaaag cggcaacacg tccacgctcg aaatcgtcga ccgtatcaag
     2881 aagatgatgc cgacgctgcg caatctcgtg ccggcttcgc tgaacatcga tccggtcgcc
     2941 gatcagtcgc tgttcgtgcg tgcttccgtg cagggcgtgc tgcgcgaggc gctgatcgcc
     3001 gcatgcctga ctggcctgat gattctgctg ttcctcggca actggcgcgc cacgctgatc
     3061 atcgccgtgt cgattccact gtcgatgatc acctcgatca ttgcactgtc gatgctcggc
     3121 gaaaccatca acatcatgac gttgggcggc ctcgcacttg cggtcgggat tctggtcgac
     3181 gacgcgacgg tggcaatcga aaacatcagc catcagctgg agcaaggcaa gacgctggag
     3241 caggcgattc tcgacggcgc gcatcagatc gcgatcccta ctttggtgtc gacgctctcc
     3301 atctgcatcg tgttcgtacc gatgtttctg ctgacgggcg tcgcgcacta tctgttcatt
     3361 ccgctcgccg aagccgtggt gttcgcgatg ctggcgtcct acttcttctc gcgcacgctg
     3421 gtgccgacgc tcgccaagta tctgctgcgc catcaccaca agccggcgga tctgcatcac
     3481 gcgccaccac aaacacgcaa tccgttcatg cgcgtgcact atgcattcga gggcggcttc
     3541 gcgcgttttc gcgaccgtta ccgcgaattt ctcgaagcgc gggtggcgcg cccgggtctg
     3601 ttcgtgacgc tgtttctggt ctgctgcggt gcctcgatgc tgctgatgcc gtttctcggc
     3661 cgtgacttct ttccggcagt cgatgcaggc acgatcgcgc tgcatctgcg cgccaaaacc
     3721 ggcatgcggg tcgaggaaac cgcggtggta accgatcgcg tcgacacgcg cattcgtcag
     3781 ttgataccgc cggccgagtt gcattcgctc atcgacaata tcggcttgcc ggtctcgggt
     3841 atcaacctct cgtacagcaa caccggcacg atcggcacct cggattcgga cattctcatc
     3901 acgctgaatc ccgatcatca tccgagcgcg gactacgtgc gcacgctgcg ccgaaccctc
     3961 accgacgaat tccccggcgt gcagttcgcc ttcctgccgg cggatatcgt cagtcagacg
     4021 ctcaacttcg gcatgccctc gccgatcgat attcaaatcg tcggacgtga cgtagccggc
     4081 aatcgcgtct tcgccgcgca gttgctgaat cgcctgcgca ccgtgccggg tcttgccgac
     4141 gcgcgcatcc agcaacccgc cgatctgccg cgcatcttta tcgacgtaga ccgcacgcgc
     4201 gcccagcagg caggcttttc gcaacgcgac atcgcgagca atctgctgat cacgctgtcc
     4261 ggcagccagc agaccacgcc gaccttctgg ctcaatccgc gcaacggcgt gagctacaac
     4321 gtgatcacgg aagcgccgca atatacgatc gattcgctgc aatcgctcgc gaacattccg
     4381 ttgaacgcga acggccgcag caatatcctc ggctcgctcg ccacaatgaa acgcgaggcg
     4441 ggcaatgcgg tgctcacgca ctacaacgca caaaccacca tcgacatttt cggcaccgcc
     4501 gacggccgcg atctcggcgg cgtatccgac gacatccgca agatcatcga cgacagcaag
     4561 gccaatctcc ccaaaggttc gagcatcgag gtgcgcggcc aggtgcagac gatgaatgat
     4621 tcgttctcag gtctgttcgc cggtctcgtg ttcgcaatcc tgctggtcta tctgctgatc
     4681 gtggtgaatt tccagtcgtg gctcgatccg ttcatcatca tcaccgcgct gcccggcgcg
     4741 ctggcgggca tcgtgtggat gctgtttctc acccacacca cgctgtcgat tccggcgctg
     4801 accggcgcga tcatgtgtat cggcatcgcc accgcgaact cgattctggt gatcagtttc
     4861 gcgcgcgaac agttgctcga acacggcgac gcgacacgcg cggcgatcga ggcgggcttc
     4921 acgcggttcc gtccggtgct gatgacggcc ctcgcaatgg tgatcggcat ggtgccgatg
     4981 gcaatcggtc tcggcgaagg tggcgagcag aatgcgccgc tcgggcgcgc ggtgatcggc
     5041 ggtttgacgg tcggcacgtt ggctacgctg atcttcgtgc cggtggtgtt ttcgatgatc
     5101 tatcgccggc ttgccgcgcg gcgtcagcga gcggccgagc acgtggtgca aaagccatga
     5161 cggccagcgc acgacacacg gtacgaaaca cgagaggcag cgtcgactgc ccaggcattc
     5221 tgatagggga agtttgatgg aaggaaaacg tccagacggg tccaactccg cgctcaatcc
     5281 ggcgaagcaa aagcgcgcac gctgggtcat cgtggcggtg gccgtggtgg tggccgtact
     5341 cgcggcacaa ggcatctggt cgcgccatga cgcgcacgca gcgctcgaac gcgatgccga
     5401 gcatgcgagc cagacaagcg tcgaagtcgt gcgcccgcag aagtcgacgg tgggactcga
     5461 cctcgtgttg cccggcaacg tgcaggcgtt tctcgacacg ccgatttacg cgcgcaccaa
     5521 cggctatctg aagaagtggt acgcggacat cggtgccaag gtgaagaacg gccagttgct
     5581 cgcggaaatc gacacgcccg aagtggacga tcaactgcgc gccgcccgcg ccgatctcgc
     5641 gaatgcggac gccaactacg cactcgcgaa aagcaccgcc gaccgttgga ctgagatgct
     5701 gaaaagcaaa tcggtttcga agcaggaaac cgacgagaag gtcggcgaca tgctcgcgaa
     5761 gaaaggcacg ctggacgccg cgcgcttcaa tgtggcgcgc ctagagaaaa cgcaatcgtt
     5821 ccagaaggtg tacgcgccgt tcgatggcat cgtaacggcg cgcaacgtcg atgtgggcgc
     5881 gctgatcgat gcgggcagtt ccggcggacc ggcgaaggaa ctgttccatg tcgcgcaggc
     5941 ggacagattg cgtgtgtatg tgaacgtgcc gcaagcgtat gcgctgcagg ttcgcgcgca
     6001 gcagaccgcg tttctgacgc tgaccgagac gccgtcgaaa cactatccgg gtacggtggc
     6061 gcgcactgcg ggcgccgtcg atccgcaaca acggaccatg ctcgtcgaag tcgatgtcga
     6121 caatcgcaat ggcgatctgc tgccgggcgc ctatgcgcag gtgcatttcg ctttgggcgc
     6181 gggcgcggca ccgtttacgc tgccaggcaa cgcgctgctg ttccggccgg acggcgtcaa
     6241 ggtagcgacc gttgatccgc agcacaaggt gaagctgctg ccggtttcgc ttgggacgga
     6301 cttcggtacg cgcgtggccg tcgcgtccgg cttgcaaggc aatgagcagg tgattctgaa
     6361 tccgcaggat tcgatcgtcg acggcgcacc ggtgcggatc gtgccgggga aagcgggggc
     6421 tggtgctggg ggtgtttcga gtgtcgaggg tgcaccgggt gcctctggcg cggccgcggc
     6481 tggtgaaggt gcagcgggcg cttctggcgc ggccgcggct ggcgaaggta caacgggcgc
     6541 ttctggcgcg gccgcgactg gcgtgcctgc tgcgcacgtc gccggtctgc aagccgtgag
     6601 gaacgcactg tgagcgcgcc ggcactgcct cgtcgtttcg cgccacggct cacacgtgta
     6661 gcgctgcttg gtgcgttcgc ctgctcgctc accgcttgca ccgtcggccc cgactacgtc
     6721 aagccgccgg ccgccactgc cgccgcctac aaggaactcg aaggcaccgg ctggaagccc
     6781 gcgcaacccg ccgacacgct cacgcgcggc gcgtggtgga gcgtctatgc ggacccatcg
     6841 ctcgacgcgc tcgagcagca ggtcgcgagc gctaaccaga acgtgcaggc cgccgaggcg
     6901 cgctttcgcg ccgcccgcgc gacagttgcg caataccgct cgaacttctt ccctatcgtc
     6961 agcgcgaacg gcgattattc gcgcacgcgg acttcggaga atgtgctgca caaatcgacc
     7021 gcgggtctca cgctgaacga ttacctcgta caagccgatg cttcgtggga accggacttg
     7081 tggggccgcg tatcgcgcag cgtcgaaggc gcgaaagccg aagcgcaggc gagcgccgcc
     7141 gacgcgcaag ccgtgctcct ctcgatgcag gccgaactgg ccacggacta cttcgaactg
     7201 cgtggtttcg atcaggaacg acaacttctc gacgacacga tcaaagccta taaggaagcg
     7261 ctcgatctga cgcaacaccg ctacgccggc ggcatcgcca ccgacgccga cgtcgcgcag
     7321 gcgcagacgc agttgcaaac cactcaggcg caagcactcg acctcggcgt gcagcgctcg
     7381 caactcgaac acgcgatcgc gatcctgatc ggccagacac cctcgacctt ttccctgccg
     7441 gttgcaccgc tcaccgtagt gccggtggtc gcggcggctg gcgtgccctc cgcgttgctc
     7501 gaacgacggc ccgacgtcgc gtcggcagag cggcacgtcg ccgatatgaa cgcgcagatc
     7561 ggcgtggcga cggcggcgtt ttttccgagt ctgattttgt cggtgacggg gggcctcgaa
     7621 gcgaccaact acagccaatg gctgttggcg ccgagccgtc tgtggtcgct cgggccgaca
     7681 ctcgccggta cgctgctcga cttcggcgga cgtgcgtcgg tgaaagagca agcccgtgca
     7741 cactacgacg aaagcgtcgc acagtaccgc cagacagtgc tgaccgcgtt cgggcaggtc
     7801 gaagacaacc tcgccgcatt gcgcgtgctt gaacaggaag ccgccgcaca agacgacgcg
     7861 gtagccgccg cgcagcgttc gctggcggtc gtgtcgaacc gctataagaa tggcgcgatc
     7921 acgtatcttg atgtcgtggt tgcgcagacc accgcgctga ccaatgagcg ggatgcggtg
     7981 tcgattgctc gccgtcgtat ggcggcgagc gtcgcgttgg tcaaggcgtt gggcggcggt
     8041 tgggacgcgt cggcgttgcc gaccgacgaa cagatcgtgc atcccaccgc gcctgcgagc
     8101 gaagcggcgc acggttagct gttcgccgtg gacagttgcg gttgtggttt aaggcacccc
     8161 aagggatgcg caaaccgcag cgagcacacc agttgcacgc gtcgcggcaa cgccccgtgg
     8221 cgtgtcgcgc ccactcgccc gaccttttca aagtatcctt aactccttcc accacctcgt
     8281 cttcccagga gtttttttcg tgaccgcacc gccggaatcc caccggactg ctacttcagc
     8341 tgttcccgct ccacccgcta cttccccctc cgctgccgtg cctgacgtcg cccacacccc
     8401 ctatctcgaa cgaggcacgc gcgcctattg gcgcgccagc atcgcgttgc tattcgccgg
     8461 ttacgccacc ttctcgctgc tgtattgcgt acagccgttg ctgcctgcat tctccaaggc
     8521 gttcagcgta acgccggcac aaagcagctt gtcgctctcg gtgacgacgg ccgcgctcgc
     8581 gctcgccata ttcgtggccg gcttcgtctc ggaaggctgg agccgccata agctgatgac
     8641 tgcatcgctc acgctgtcgg cgctattgac gatcggcgtc tcgatcgcgc cgcagtggca
     8701 tcaactgctc gtgttgcgcg cgctcgaagg cctcgcgctg ggcggcgtgc ctgccgtcgc
     8761 gatggcgtac ctcgcggagg aagtgcaccc cgatgggctc ggtctggcga tgggactgta
     8821 cgtcggcggg acggcgatcg gcgggatggc ggggcgggtg attaccggcg tcgtcgcgga
     8881 tctgttttcg tggcgcgtgg caatcggcac gatcggcgtg ctcggcattc tctccatgct
     8941 cgcgtttcgc gcgctgctgc caccgtcgcg ccatttcgtg ccgcgtcgcg gactcggctt
     9001 tgcccatcac cacgccgcgt tgctgaagca attcacgcgg cccgggttgc ccctgctgtt
     9061 tctgctcggc ttcgtgctga tgggcagctt cgtcacgcta tacaactaca tcggttaccg
     9121 gctgcttgcg ccgccctacc tgctgagcca aacagagatc ggcgcgattt tcgtcgtcta
     9181 tctgacgggc gtgatcgcct cgccgtggtc gggccgcatg gccgatacgt tcggccgggc
     9241 acgcgtgctg atcggcagct tgctgctgat ggcgctcgga ctcgccctca cgttgctcca
     9301 tccgatcgcg gcaattgcaa tcggcatcgc ctgcttgacg ttcggctttt tcgccggaca
     9361 ttcggtggca agcggctggg tcggacgcct cgcgaaagaa gccaagggac aggcggccgc
     9421 gctgtatctg ctcgcgtatt acatcggctc cagcgtggtc ggttcgtatg gcgggcacgt
     9481 gtgggccgct tatggctgga acggtgtcgc gggactggtg ggcgtgctgc tcgtcgtcgg
     9541 catcgttgcg gcagtgcgct tgcgtcaacg cgaagagacc gcaggatgaa ggcatgccgc
     9601 ggcccggttt cgggaaaccg ggctcggacg cgacccggag tcaggcctaa aaagccgcgc
     9661 gtcgcagcag acgagaacaa tccgccgcgc atcaaattgg cttaaaaagt aaggctcatg
     9721 taagcgaaca cttcattttt tacgacgttt ttgctgcggc gcgtcaatcg ccgcggccct
     9781 tgcagcattg agttcagcag tctgcgaaga cgcgctgcag cgccgcaagc cggtgttatg
     9841 ttgatctaac cgtcgcctat actcgattca ggcgctggcg gtgcatgact ccagaaatag
     9901 agccgctgcc cgcgccggta ctagacaagg agacgagtat gacgacctac ttcacgattg
     9961 gcgatttcat cctggtcatc ccgatggcgc tggccggcgc tctgtttgtc ggcgcgcttc
    10021 cctgcaagac ggagttgcgt cacaacgcgc tacgcgtgct aggcgcgctg atcggcgtgg
    10081 tgttcgcggt cgtgctggtc gaaggtttgc ctgcgctggt gtagcggggt ccggcgcaga
    10141 cggccaggac gcttcgggga tagttcgatc cggatagttc ggttgactga acgcagtcac
    10201 tttggtcgct gcgtgggaaa cgtgtgtatc tggacacctt gattaacaaa gccgccccag
    10261 cgggcggctt tgctattgct acgacgaaat tcccacgact gaaacgctac gcggtcagat
    10321 cgcctgatcg gtcgaaggct tttcccacag attgacgcca ccttcagttg catagcggtc
    10381 gatctccgcc agctcttcct tcgagaacgc gagattcttc aacgcaccga cgttctcgcg
    10441 cacttgctcc gcacggctcg cgccgatcag cacggacgtc acccgcggat cccgcagcgc
    10501 ccatgcgagc gccatttgcg caaggctttg tccgcgacgc tgcgcgatgt cattgagctt
    10561 gcgcacgtgc tcgatgtttt gcgggctcag atgctcctgc ttcaacgaac cgccgcccgg
    10621 cttgttgacg cgcgcgtctt ccggcacgcc attcagatat ttgccggtga gcaggccctg
    10681 cgcgagcggc gtgaacgcga tcgcccccgc gccggccttc tcgagcgcat ccagcagttc
    10741 gcgttcgatc cagcggttga gcatgttgta cgccggctga tggatcagaa gcggcacctt
    10801 gtactcggcg agcagtctgg ccatttcgag cgtcttggtt gccgagtacg acgaaatacc
    10861 gatgtacagc gccttacctt gctgcacggc gctcgccaat gcaccggcgg tttcttcgag
    10921 cggcgtatcg gcatcaaagc gatgcgaata gaaaatgtcg acgtaatcga gacccatgcg
    10981 ttgcaggctc tgatcgagac tcgcgagcac gtacttgcgc gagccgccgc cctggccata
    11041 aggacccggc cacatgtccc aacctgcctt cgacgagatc agcagttcgt cgcgatacgg
    11101 cttgaagtca tctttgaaga gacggccaaa gttggtttcg gcgctgccgt acgggggacc
    11161 gtagttgttg gcgaggtcga agtgagtgat gcccagatcg aaggccgtgc gcaggatatc
    11221 gcgctgtgtg gagatcggcg tggtgtcgcc gaagttgtgc cacaggccca gcgacagcgc
    11281 cggcagtttg agacccgatt tgccgcagac gcggtattgc atgtccgaat agcgttctga
    11341 agctgcttcg taagccattg ctagtgtcct tgatggtgag gtcgcccggc ggttgcgggc
    11401 gtgggaagat cacatgctgc gagaggcgtg ccggttctac gtttaggtat gagtgacgct
    11461 agaccgctat ggtagccaat cgacgtgatg cctgcagacg cccacagcat gcaagatgga
    11521 cgattgcttg cagctcccct acactttggc cgatcaaggc ttatcgaagg ggtggattca
    11581 tgacgaaggc gatttcaatc gcgctggttg tcggcggtat cgtgctgttg tatttcggcg
    11641 ggcaggcgtt caactcggtc agcagcgagg tgtctcgcgt ctttaccgga tcgccgacca
    11701 acaaggcgat catgctgctc gtgggcggtg ttatcgccac gattgccggc ctgaccggga
    11761 tggctgtttc agggcgcaag cggtagagcg ttaacgctta cgcggcgcga gaccaacaca
    11821 cattcgcgcc gcgccaggca aaaaacctaa aaggcaattt ccggcttgcc ggccgagcgg
    11881 gatcccggca gcggcacatt gctcgccccg tgataggtaa acaccaaaga aaatttcacc
    11941 tgatcgctga ggttcttgcc ggccgaatgc agcgtgttgc agtgaaagaa caccacgtcc
    12001 ccggccttga gctcgggcga aacggcggtg cgaatcaagg cctgattttc ttccagatcg
    12061 gagcggaaga acttggcctc gtcgaagcgc tcggaagtga aggcagcgct gtgcgacttc
    12121 ggcacaaacc acaacccgcc gttatcgacc gtctccgacc ctactgcgac ccacaccgat
    12181 accaggtcgt cacgctcgaa tgaccaatac cgcacgtccc gatgccagcc ggtcaggctg
    12241 ccatacgcgg ggtgcttggt catcatgcaa ttgtgatgcg cgcgcgataa gcgcggctct
    12301 tcaccgaagt acaactccat ccagccacga atttccggcg cggtcgccca ttgcgcaaaa
    12361 ctcggatgac ggccgtacgc atccagcaag cggcgaaccg tgtggccacc cggcgcatgt
    12421 ttggattccg gcgcgcccgg atactgcaga tctgcttcga actcgatcgg cgcggccgct
    12481 tcctgcaact ggcgctccgc aatctgcttc agttcgccgc aacgctcggg cgacaccaat
    12541 ccgggcacga cgacaaaacc ctgctctcgc aacgtgtgaa tctgctcttt cttcgaatga
    12601 agtgacatgg tgttccgtta ctgtcggcta gctgatgctc gattgtaaaa cgggcgcgat
    12661 cggactgcgc gttgtttcgc cttgcgcgaa gccgcagcct tacgcactac tttcatgcga
    12721 aagccgattt gcagagcacg ctgtgagggc acgctgcacg ataatcgacc gcaaagggcg
    12781 cgaccctgca agcgtgcacc tcacgactta cccgtaaaaa cccgtattcg cagggtaaaa
    12841 acggactttc tgacgtcacg aatcgcgtgt agcctttcac gcatgttgat cgctcttccc
    12901 gcactttcac ggcgcacaca ggccgctgtg gccgactccg ccgtcctccg tgttcttatc
    12961 agtcgaattc cgggctctcg cgccgtcctt cgcggcaccg ccattggcac atttggtgct
    13021 ccttctggcg cacactccgt cgcgcgacac gttgccaacc tgtgaagcca tgcatactct
    13081 tctgagcacc cgtcttaccc gcgccgcgtt gaaagcggcg cgtccggcgc gtcgccatct
    13141 cgtgcgcgcg ctgcgtcacc atgccacccg cactcgcgca ttaggcgaac aatggcgtga
    13201 agccaaagcg gtcgacggca ttcgcacgtg gttccacacc ttcttctccg cactgcgcct
    13261 gcgcggcagt tcgcggcgct tttcgctgcg cacgctgctg cgcaaaccga cgccgctgcg
    13321 ccttaccgcc aagcagactg ctccggtacc agcaccgcaa agcacgagcc gccgcccgcg
    13381 acgtatgtcg acgagcggca gcaccggctg gtttgcgttt gcgtttgcga gtcgctgaat
    13441 gagcgactag gtggaaggct ttgccggtcg ctgaatgagt cgctgaatga gcggccgaat
    13501 aggcgactgc gccgcgcatg cgcggccagg cgagtcgttg agagcgaacc gcgagccagc
    13561 ctcgaacgca cccccactcc cgatggaagc gggtacggac ggctgcggcc gcattgacgc
    13621 cctcatcgcc ctagggcaca gcgtcttcga tggcaataaa agaccccgcc cggctcgcgc
    13681 cggcggggtt ttttactaac gcggactgcg cgcttattcc gcgagcgcgg cgaccggttc
    13741 ggtgcctgct gcttcggcga gtgccagagc cttgtccgtg gcttcccacg tgaattccgg
    13801 ctcttcgcgg ccgaagtgac cataggccgc gctct
//
LOCUS       DATASH010000985         3249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2542628,
            whole genome shotgun sequence.
ACCESSION   DATASH010000985 DATASH010000000
VERSION     DATASH010000985.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2542628"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(252..1559)
                     /locus_tag="VHV99_26260"
     CDS             complement(252..1559)
                     /locus_tag="VHV99_26260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HEX3638922.1"
                     /translation="MADSYFPRWRVQPNAAEGRIVGTDERLAWPQMFAMGIQHVVAMF
                     GSTVLAPLLMGFDPNLCIFMSGIGTLLFFVLVGGRVPSYLGSSFAFIGLVIAVTGYGG
                     HGPNMNIPVALGGIIACGVVYAIVGLIVSAVGTKWIETLMPPVVTGAIVCVIGLNLAP
                     IAVHGVSGSNFDSWMALVTVLCVGAVAVFARGMLQRLLILIGLLMAYVIYAIVTNGLG
                     MGKPIDFAIVANAAWFGMPHFTAPVFNVQAMTLLAPIAVILVAENLGHIKAVSAMTGQ
                     NLDRYVGRAFLGDGLATIVSGFAGGTGVTTYAENIGVMAVTKIYSTLVFVIAAVIALV
                     LGFSPKFGAVIQTIPGPVLGGVSIVVFGLIAVTGARIWVVNKVDFSDNRNLIVAAVTL
                     VLGAGDFSLKLGGFGLGGIGTATFGAIILYALLRREPARGPVA"
     gene            complement(1888..>3249)
                     /locus_tag="VHV99_26265"
     CDS             complement(1888..>3249)
                     /locus_tag="VHV99_26265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX3638923.1"
                     /translation="AANLGYATDDAGRQKLKNGIVASLKEMHGAYDQYRATIATSKGQ
                     EMFDEVLRKSAVWEGIVHQQIALEAIPAGVDNNELVRRAIASSEALRDRITALIDYRR
                     QQANEAQQEASADYQHMRIVMLALVLGAIAAGTLLATMIARRLARQLGGEPDYAMHIA
                     NRIADGDLSVRVDTRAGDTDSMLFALRNMRERLAGIVSGISESSESILMASGEIAQGN
                     TDLSQRTEEQAAALQETASSMQELTSTVKLNAENAQQAGGVAHGASEVAARGSHLVGD
                     VVETMRELAAGSKRMTDIIVVIEGIAFQTNILALNAAVEAARAGEQGRGFAVVAGEVR
                     SLAQRSAVSAKEIKELIESSTARVGSGAELAERAGQTMAEVTHAVKRVTDIMGEISSA
                     SQEQSTGIEEVNRAVAQMDDVTQQNAALVEQAAAAAASMADQARQLQAAVTVFSLDAA
                     RHG"
BASE COUNT          701 a         1165 c          863 g          520 t
ORIGIN      
        1 ttgagaatcc agcacccaga aacagtttct cgcttgactt ccaatacggt tgcttttctt
       61 tgcggcaggc aaagaaaagt aggtgcccgc cccgcacagg ggcgacgcta atagaccaat
      121 atcaattcaa ggaaaggcca aaaacccaga tcaaggaaag gcccacacca taaaagcaca
      181 gacaaacaag cgccgcacag gcaaccagat caagcaaaca gaaacaagcg cgagcaacca
      241 aaaacccaac atcaagccac cggcccccgc gccggctccc tcctcaggag agcataaaga
      301 atgatcgcac cgaacgtagc ggtcccaatc ccaccaagcc caaacccgcc aagcttcaac
      361 gaaaaatccc cagcccccag caccagcgtc accgccgcaa caatcagatt ccgattgtcg
      421 gagaaatcca ccttattaac gacccaaatc cgcgcgccag tcaccgcgat caaaccgaac
      481 acaacaatcg acaccccgcc gagcaccggc cccggaatgg tctgaatcac cgccccaaac
      541 ttcggcgaaa agccaagcac cagcgcaata accgcagcaa taacaaacac cagtgtcgaa
      601 taaatcttgg tcacggccat cacgccaatg ttctcggcgt aagtcgtcac cccggtgcca
      661 ccggcgaaac cggagacaat cgtcgcgagt ccatcgccga gaaatgcacg gccgacgtaa
      721 cgatccaggt tctgaccagt catcgcactc accgccttga tgtgcccaag attctccgcc
      781 accagaatca cagcaatcgg tgcgagcagc gtcatcgcct gcacattgaa aaccggcgcc
      841 gtgaagtgcg gcataccgaa ccacgcagca ttcgccacaa tcgcaaaatc gatcggctta
      901 cccatgccga gtccattcgt cacgatcgca tagatgacat acgccatcag caaaccgatc
      961 agaatcagca aacgctgcag catgccgcgc gcaaacaccg cgaccgcgcc gacgcacaag
     1021 acggtcacca gcgccatcca cgaatcgaag ttgctgccgc tcaccccatg caccgcgatc
     1081 ggcgcaagat tcagcccgat cacgcagacg atcgcgcccg tcacgacagg cggcatcagc
     1141 gtttcaatcc acttcgtgcc gaccgccgag acgatcaggc cgacgatcgc gtacaccacc
     1201 ccgcacgcga tgatcccgcc caacgccacc ggaatgttca tgttcgggcc gtggccgcca
     1261 taccccgtca ctgcgatcac aaggccgatg aacgcgaagc tcgagccgag gtagctcggt
     1321 acgcgcccac ccaccagcac gaagaacaac agcgtgccga tccccgacat gaagatgcac
     1381 aggttcggat cgaagcccat cagcaacggc gcaagcacgg tcgagccgaa catcgcgacg
     1441 acgtgctgaa tacccattgc aaacatctgc ggccacgcaa gccgctcgtc ggtgccgaca
     1501 atacggccct cggcagcgtt cggctggacg cgccagcgtg ggaagtagga atcggccatg
     1561 gcgggggttc tcctctgtcg gcgttttagt ggacggcgcc gcgaactgcg ccgccggtga
     1621 attacgcgcg agtgtacgga gagccaccag gcctggcaag cgcgacaact tgcatgcctg
     1681 aaaccgcgca atgggcgcta cggacgcaac gccctcgaat atcatggagg taaccacgca
     1741 ccgccctgaa ctatcaccga cgcaagcagt cacgcccgcg acgatcacca gctcgagcag
     1801 cacgccactg atccatcacc ggcgcaacag ttacgccctt acgacgacct gcgcgatcag
     1861 ccgcgccgct caccatcggc cactcaatca gccgtgccgt gccgcgtcca gcgagaacac
     1921 cgtgaccgct gcttgcaact gcctcgcctg gtccgccatc gacgccgccg cggccgccgc
     1981 ctgctcgacc agcgccgcgt tctgctgggt cacgtcgtcc atctgcgcaa ccgcgcggtt
     2041 cacttcctcg atgccggtgc tctgctcctg cgaggccgac gaaatttcac ccatgatgtc
     2101 ggtcacgcgc ttcaccgcat gggtgacttc cgccatcgtc tgaccagccc gctcggccag
     2161 ttcagcgccg ctgcccacgc gcgccgtcga gctctcgatc agctccttga tctccttggc
     2221 cgacaccgca ctgcgctggg ccagtgaacg cacttccccc gccaccacgg caaagccgcg
     2281 tccctgttcg ccggcacgcg ccgcttccac cgctgcattc agcgcgagaa tgttggtctg
     2341 aaacgcgatc ccctcgatca ccacgatgat gtcggtcatg cgcttcgagc cggccgccaa
     2401 ctcgcgcatc gtctcgacca catcgccgac gaggtggctg ccgcgcgccg ccacttccga
     2461 cgcgccatgc gcgacgccgc ccgcctgctg cgcgttctcg gcgttcagct tcaccgtcga
     2521 cgtcaactcc tgcatgctcg acgcggtttc ctgcaacgcg gcggcctgct cttcggtgcg
     2581 ttgcgagaga tcggtgttgc cctgcgcgat ctcgcccgag gccatcagga tcgattcgct
     2641 cgactcgctg atgcccgaca cgatgccggc cagccgctca cgcatgttgc gcagcgcgaa
     2701 cagcatgctg tcggtgtcgc ccgcacgagt gtcgacgcgc acgctcaggt cgccgtcggc
     2761 gatacggttg gcaatgtgca ttgcgtaatc cggttcgccg ccgagctgac gcgcgaggcg
     2821 tcgcgcaatc atcgtcgcga gcagcgtgcc cgccgcgatc gcgcccaaca ccagcgccag
     2881 catcacgatg cgcatgtgct gatagtcggc gctcgcctcc tgctgtgctt cgttggcttg
     2941 ctgacggcga tagtcgatca gcgccgtgat gcgatcgcgc agcgcctcgc tcgacgcaat
     3001 cgcgcggcgc acgagttcgt tgttgtcgac gccggccgga atcgcttcga gagcgatctg
     3061 ctgatggacg atgccctccc acacggccga cttgcgcagc acttcgtcga acatctcctg
     3121 acccttgctg gtcgcaatgg tcgcgcgata ctgatcgtag gcgccgtgca tctccttgag
     3181 cgatgcgacg atgccgttct tcaacttttg tcggccggca tcgtcggttg cgtagccgag
     3241 attcgcggc
//
LOCUS       DATASH010000986         7774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1357474,
            whole genome shotgun sequence.
ACCESSION   DATASH010000986 DATASH010000000
VERSION     DATASH010000986.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7774
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1357474"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..141
                     /locus_tag="VHV99_26270"
     CDS             <1..141
                     /locus_tag="VHV99_26270"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017333983.1"
                     /note="Transfers the gamma-phosphate of ATP to the 4'
                     position of a tetraacyldisaccharide 1-phosphate
                     intermediate to form tetraacyldisaccharide
                     1,4'-bis-phosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEX3638924.1"
                     /translation="ILITEKDAVKLGSWHDARIWVVPVEAALDHRLIALVVEKVRGRS
                     PA"
     gene            122..325
                     /locus_tag="VHV99_26275"
     CDS             122..325
                     /locus_tag="VHV99_26275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEX3638925.1"
                     /translation="MDARLLEILVCPICKGPLSYDRSAQELTCNADKLAYPIRDGIPV
                     MLVDEARQTVEGTPVDLNPGSVA"
     gene            493..1293
                     /gene="kdsB"
                     /locus_tag="VHV99_26280"
     CDS             493..1293
                     /gene="kdsB"
                     /locus_tag="VHV99_26280"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEX3638926.1"
                     /translation="MTNANATTPPFIAVVPARLASTRLPNKPLADIGGKPMVVRVAER
                     ARESGAQQVLIASDAQAVLDVAREHGFEAVLTRADHPSGTDRLAEVAAQFGWSDETIV
                     VNVQGDEPLIDPALVCGVASHLASSQGCAIATAAHPITDPAEIFNPNVVKVVLDARGV
                     ALYFSRAPIPWARDAYQPHWPNVASMPAPPAPAVVHRHIGLYAYRAQFLRTYPGLAIS
                     PIEQIEALEQLRAMWHGERIAVLVTQNAPLPGVDTPADLARVQALFRS"
     gene            1510..2175
                     /gene="adk"
                     /locus_tag="VHV99_26285"
     CDS             1510..2175
                     /gene="adk"
                     /locus_tag="VHV99_26285"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEX3638927.1"
                     /translation="MRLILLGAPGAGKGTQANFIKEKFGIPQISTGDMLRAAVKAGTP
                     LGLEAKRFMDAGELVTDELIINLVKERLQQPDCANGYLFDGFPRTIPQAEAMKHADVA
                     IDYVLEIDVPFDEIIVRMSGRRSHAASGRTYHVKFNPPKVEGVDDVTGEALIQREDDK
                     EETVKKRLDVYVAQTKPLIEYYNSWAENGDSSTSLKAPQYRRISGLGTVDEIRDRAFD
                     ALK"
     gene            2480..3238
                     /locus_tag="VHV99_26290"
     CDS             2480..3238
                     /locus_tag="VHV99_26290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEX3638928.1"
                     /translation="MEIPDNVFLITGGASGLGAATARLLVENGGKVVLADLNQDAGEA
                     LAKELGGVFVKCDVSREDDATEAVEAATKLGTLRGLVNCAGVAPAIKTVGKDGPHPLE
                     SFARTISINLIGTFNMIRLAAAAMSKNEPNANGERGVIINTASVAAYDGQIGQAAYAA
                     SKGGVVGMTLPIARDLSRNAIRVMTIAPGIFETPMLLGMPQEVQDALGAMVPFPPRLG
                     KPAEYAMLAKQIFDNPMLNGEVIRLDGAIRMQPK"
     gene            complement(3419..4261)
                     /locus_tag="VHV99_26295"
     CDS             complement(3419..4261)
                     /locus_tag="VHV99_26295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB1 family protein"
                     /protein_id="HEX3638929.1"
                     /translation="MTRVLDYFSTLVAEDDSLPLTEAALSLAQDAYPDLDLQGTLAEI
                     DELVVRLQRRMPDDADIRQKVGILNRFFFRELGFASNLNDYYDPDNSHLNAVLKRRRG
                     IPISLAVLYLEMAEQIGVPARGVSFPGHFLLRVTTPDGDVMLDPTSGHSLSESQMVDM
                     LEPYVASAGESVSRALRMLLQPATRREIIARMLRNLKSTYLQTERWQRLLAVQQRLVI
                     LLPESIEEVRDRGFAYARLDYLRPALEDLERYLGDRPDAEDATVVESQLHELRQRTQY
                     DDRD"
     gene            complement(4267..5817)
                     /gene="murJ"
                     /locus_tag="VHV99_26300"
     CDS             complement(4267..5817)
                     /gene="murJ"
                     /locus_tag="VHV99_26300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEX3638930.1"
                     /translation="MNLFRALLTVSGFTLLSRVTGLARETLIARAFGASQFTDAFYVA
                     FRIPNLLRRISAEGAFSQAFVPILAEFKNQQGHDATKALVDATSTVLAWALAILSLIG
                     VVGASGVVFIVASGLAHEGEAYALAVTMTRIMFPYIIFISLTSLASGVLNTYKNFSLP
                     AFAPVLLNVAFIVAAVFVAPRLQTPVYALAWAVIAGGVLQFLVQLPGLKKIDMIPRIG
                     LNPVKALAHRGVKRVLSKMVPAMFAVSVAQISLIINTNIASRIGPGAVSWINYADRLM
                     EFPTALLGVALGTILLPSLSKAHVDADPHEYSSLLDWGLRVTFLLAAPSAVALFFFAQ
                     PLTATLFHYGKFDGNSVVMVARALSAYGIGLIGLILIKILAPGFYAKQDIKTPVKIGV
                     GVLIVTQLSNYLFVPIFSHAGLTLSVGLGACVNALFLFLGLRKRGIYMPSHGWLKFFV
                     QLLGACLVLAGTMHWLAISFDWIGMHSRPVDRIVLLGACLVLFAALYFGMLWLMGFKY
                     AYFKRRVK"
     gene            6439..6717
                     /gene="rpsT"
                     /locus_tag="VHV99_26305"
     CDS             6439..6717
                     /gene="rpsT"
                     /locus_tag="VHV99_26305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEX3638931.1"
                     /translation="MANTAQARKRARQAAKANSHNSALRSKFRTAIKAVRKAIEAGDK
                     AKAAEIFQASSKTIDIIADKKIVHKNKAARNKSRLAAALKGLQAPAAQ"
     gene            complement(6939..7286)
                     /locus_tag="VHV99_26310"
     CDS             complement(6939..7286)
                     /locus_tag="VHV99_26310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3579 domain-containing protein"
                     /protein_id="HEX3638932.1"
                     /translation="MAEAAPTEYFIQGITSTGKKFRPSDWSERLAGVMACFGPSPRGK
                     GPNAYLQYSLYVRPTMIGDLKCVILDSRLRDVEPMAFDFVLNFAKDNDLLVTEACELQ
                     LEHATPLAKRHVI"
BASE COUNT         1586 a         2447 c         2331 g         1410 t
ORIGIN      
        1 atcctgataa cggaaaagga tgcggtaaaa ttggggtcct ggcacgacgc gcgcatctgg
       61 gttgtcccgg tcgaagccgc gctcgatcat cgcctcattg cattagttgt ggagaaagtc
      121 cgtggacgct cgcctgcttg aaattctcgt ctgcccgatc tgcaagggcc cgctcagcta
      181 cgaccgttcc gcgcaggaac tgacctgcaa cgcggacaag cttgcctatc cgatccgcga
      241 cggcattccc gtcatgctgg tcgacgaagc gcggcaaacc gtggaaggca cgccggtcga
      301 tctgaatccg ggctccgtcg cctgagtcag ctttgcccga tgcagggcgc tgggttccgc
      361 gttctgcgcg ccccgcgctt tggtctgcat cattgatcag cggtcgcgat ccgcatttgc
      421 cgtctttccc gtcagcgccc gcggtgtgct cgtgagcgct tccttccggt ttcatcgccg
      481 cccccccttc cgatgaccaa cgccaacgcc actactcccc ccttcatcgc cgttgtgccg
      541 gcccggctcg cctcgacgcg tctgcccaac aagccgctcg ccgacatagg cggcaagccg
      601 atggtggtga gggtcgccga acgcgcacgc gaatcgggcg cgcaacaggt gctgatcgcc
      661 tccgacgcgc aagcggtgct ggatgtggcc cgcgagcacg gcttcgaagc cgtgctgacg
      721 cgcgccgatc acccgtcggg caccgaccgt ctcgcggaag tggcggcgca gttcggctgg
      781 agcgacgaga cgatcgtggt gaacgtgcag ggcgacgagc cgctgatcga cccggcgctc
      841 gtgtgcggcg tggcgtcgca cctcgcttcg agccaaggat gcgcaatcgc caccgccgcg
      901 catcccatca ctgacccggc ggaaattttc aatccgaacg tcgtcaaggt cgtgctcgac
      961 gcgcgcggcg tcgcactgta cttctcacgc gcgccgattc cgtgggcacg cgacgcgtat
     1021 cagccgcatt ggcccaatgt ggcgtcgatg cccgccccgc cggcgcccgc ggtcgtccac
     1081 cggcacatcg gcctgtatgc ctatcgcgcc caattcctgc gcacctatcc aggtctcgcg
     1141 atctcgccaa tcgagcagat cgaggcgctc gaacaactgc gcgcgatgtg gcacggtgaa
     1201 cgaatcgcag tgctcgtcac tcagaatgcg ccgctgcccg gcgtcgacac gcccgctgat
     1261 ctcgcgcgcg tgcaggctct attcaggtct tgagcgtaaa aacccatggc ataatcggtt
     1321 ggttgtgcgc gcctcccgtt aaacgcgaga gtcagggttt gccccggtcg cgcgccgtgt
     1381 tcatcttgca gcgccgtcgt gttcgacgtg cagcgcgaga ctcgaaccgg cagctgccgc
     1441 gggtccctcg acgaatcgtg ggatgactgc agcgccaaca aagaattcac atagatctgg
     1501 agatatcaca tgcgtttgat ccttttgggt gcacccggcg cgggtaaggg cactcaggca
     1561 aacttcatca aggaaaagtt cggcattccg cagatttcca ccggtgacat gctgcgcgca
     1621 gccgtcaagg caggcacgcc gcttggcctc gaagcgaaac gcttcatgga tgccggcgaa
     1681 ctggtcacgg acgagctgat catcaacctg gtgaaggaac gcctgcagca gccggattgc
     1741 gcgaacggtt atctgttcga cggttttccg cgcaccattc cgcaagctga agccatgaag
     1801 catgcggacg tcgcgatcga ttacgtgctg gaaatcgacg tgccgttcga cgagatcatc
     1861 gtgcgcatga gcgggcgccg ctcgcacgcc gcgtcgggcc gcacgtacca cgtcaagttc
     1921 aacccgccga aggtcgaagg tgtcgacgac gtgacgggcg aagcgctgat ccagcgcgaa
     1981 gacgacaagg aagaaacggt caagaagcgc ctcgacgtgt acgttgccca aaccaagccg
     2041 ctgatcgagt actacaacag ctgggcggaa aacggcgatt cgtcgacctc gctgaaggcg
     2101 ccgcaatatc gccgcatttc gggcctcggc acggtcgacg aaattcgcga ccgcgcgttc
     2161 gatgcgttga aataattctt cgcatcagcg ttggttgttc gaaagacccg cccgattccg
     2221 gcgggttttt ttatggcgcg gcgcacggcg cacggcgcac gtgcttgtgc gtaattgcgt
     2281 ggtcttgcgc gctctccttt ttccgccggc ttccgtattg attagccatc catagcggtt
     2341 tgcaggcgca aggccgcgcc acgccctacc cgctgcccca cccgtcgctt cgctttccgc
     2401 gctgcagcat agccgttaca atcgatcatc gaaaagatat tcgatcgaga cattactgaa
     2461 ccgaagcaaa ggagaagaca tggagattcc tgacaacgta ttcctgatca ccggcggtgc
     2521 atcaggcctc ggcgcggcca cagcgcgcct tctcgtcgag aacggcggca aggtggtgct
     2581 cgccgatctg aatcaggatg ctggtgaggc actggcaaag gaactcggcg gcgtgttcgt
     2641 caaatgcgac gtgagccgcg aagacgacgc caccgaagcc gtcgaagccg ccacaaagct
     2701 cggcacgctg cgcggcctcg tcaattgcgc gggcgttgcg ccggcgatca agacagtcgg
     2761 caaagacggt ccgcatccgc tcgaatcgtt cgcgcgcacc atctccatca atctgatcgg
     2821 cacctttaac atgatccggc tcgccgccgc ggccatgtcg aagaacgaac cgaatgcgaa
     2881 tggcgagcgt ggtgtgatca tcaacaccgc atcggtcgct gcgtatgacg gccagatcgg
     2941 ccaggccgct tatgcggcgt ccaagggcgg cgtggtcggc atgacgctgc cgatcgcacg
     3001 cgatctgtcg cgcaacgcga ttcgtgtgat gacaattgca ccaggcatct tcgaaacgcc
     3061 gatgctgctc ggcatgccgc aggaagtgca ggacgcgctt ggcgcgatgg tgccgttccc
     3121 gccacgtctc ggcaaaccgg cagagtacgc gatgctggcc aagcagatct tcgacaatcc
     3181 aatgctcaac ggcgaagtga ttcgcctgga cggcgcgatc cgcatgcagc cgaagtaagg
     3241 ctggatcccg cgtgccgctt gatgcggccg cgccaatgaa aaacgcctgc gcatgagtca
     3301 tgtgcaggcg ttttgatttg accgggtttt gctcatcaac ggcggctgga cgcagccgct
     3361 gattccttgc cttcgctcct tgagctatga gcctgatccg cgggcgcaca aagcgctttt
     3421 agtcgcggtc gtcgtactgg gtgcgttgac gcagttcatg taactgcgac tccaccaccg
     3481 tcgcgtcttc cgcatccggc cggtcgccga gatagcgctc gagatcctcg agcgccggac
     3541 gcaggtaatc gagccgcgca taggcgaaac cgcgatcgcg cacttcctcg atgctttccg
     3601 gcaacaggat cacgagacgc tgctgcacgg cgagcaaacg ctgccagcgt tccgtctgaa
     3661 gataagtcga cttcagattg cgcagcatcc gcgcgataat ctcgcggcgc gtggccggct
     3721 gcagcaacat gcgcagcgcc ctgcttaccg actcacccgc cgacgccaca tacggctcca
     3781 gcatgtccac catctgcgat tcggacagcg aatgcccgct ggtcggatcg agcatcacgt
     3841 cgccatccgg cgttgtcacg cgcagcagga agtggcccgg aaacgacacg ccgcgcgccg
     3901 gaacgccgat ctgttcggcc atttccagat acagcacggc cagcgaaatc ggaatgccac
     3961 ggcgccgctt gagcaccgcg ttcagatggc tgttgtcggg atcgtaataa tcattgaggt
     4021 tgctggcgaa acccagctcg cggaaaaaga accggttcag aatgccgacc ttctgccgga
     4081 tgtcggcgtc gtccggcatg cggcgctgca agcgcacgac caactcgtcg atctcggcca
     4141 gcgtgccttg caaatcgaga tcgggataag cgtcctgcgc gagcgagagg gccgcctcgg
     4201 tcagcggcag gctgtcatct tcggcaacca gcgtgctgaa atagtcgaga actcgcgtca
     4261 tcgtgatcac ttcactcgcc ttttgaaata cgcgtacttg aagcccatca gccaaagcat
     4321 accgaaatat agcgcggcaa acagaacgag gcacgcaccc agcaacacga tacggtcgac
     4381 tggccggcta tgcatgccga tccagtcgaa gctgatcgcg agccagtgca tcgtaccggc
     4441 cagcaccaga caggcgccga gcaactgcac gaaaaacttc agccagccat gcgacggcat
     4501 atagatgccg cgtttgcgca ggccgaggaa caggaacaat gcattgacgc aagcgccaag
     4561 cccgacgctc agcgtaaggc ctgcatgcga aaaaatcggc acgaacaggt agttgctcag
     4621 ctgcgtgacg atcagcacac cgacgccgat cttcaccggc gtcttgatgt cctgcttcgc
     4681 gtagaaaccg ggcgcgagga tcttgatcag aatcaggccg atcaggccga tgccgtaggc
     4741 cgacagcgca cgggcgacca tcaccaccga gttgccgtcg aacttgccgt agtggaacag
     4801 cgtggcggta agcggctggg cgaagaaaaa caacgcgacg gcgctcggcg cggccagcag
     4861 gaaggtgacg cgcagccccc agtcgagcag cgacgaatac tcgtgcggat cggcatccac
     4921 gtgcgccttc gaaaggctcg gcagcaggat cgtgccgagc gcgacgccga gcagcgcggt
     4981 cgggaactcc atcagccggt cggcgtagtt gatccaggag accgcgcccg gaccgatgcg
     5041 cgacgcaata ttggtgttga tgatcaggct gatttgcgcg accgacacgg cgaacatcgc
     5101 cggcaccatc ttcgacagca cccgcttcac accgcgatgc gcgagcgcct tcaccggatt
     5161 caacccgatg cgcggaatca tgtcgatttt cttcagaccc ggcagttgca cgaggaattg
     5221 cagcacaccg ccggcgatca ctgcccacgc gagcgcataa accggcgttt gcaggcgagg
     5281 cgcgacgaac accgccgcga cgatgaaggc gacgttcagc aggaccggcg cgaacgcggg
     5341 cagcgaaaag ttcttgtacg tattcagcac gccggacgcc agcgacgtca gcgagatgaa
     5401 aatgatgtac gggaacatga tgcgcgtcat ggtgaccgcg agcgcatacg cctcaccctc
     5461 atgcgcgaga cccgaggcga cgatgaacac cacgcccgac gcccccacca ccccgatcag
     5521 cgagagaatc gcgagcgccc aggcaagcac cgtcgacgtg gcatcgacta gcgccttggt
     5581 ggcgtcgtgc ccttgctggt tcttgaactc ggcgaggatc ggcacgaagg cctgcgagaa
     5641 agcgccctcg gcggaaatgc ggcgcagcaa gttcggaata cggaaggcga cgtaaaacgc
     5701 gtcagtgaat tgactggcgc cgaacgcgcg agcgatcaac gtttcgcggg ccagtccggt
     5761 cacgcgcgac agcagcgtga agccgctgac cgtcagcagg gctcggaata gattcatggg
     5821 gcgcttatta tacgggtgcc gcaggtgcgg acgacgagcc ggaacgcgaa tcggaccgat
     5881 ttgcgccgca gacgttcgtt cggatgcgtg gatcgggttg tttttttgtt gcttgcggca
     5941 cacgttgcgc gcgggaacaa ccaaaggtgt cgcactggtt cgacgcgctg acgcgcggtg
     6001 tgggagtggc ggttgccccc gttcttctcc cggtgggctc cgatggcaca cccacagccc
     6061 gcccctttcc ccagcccctc cccattgagg cttacaaatc caggggcatg tccggcaggg
     6121 cctgcgcatc gaattggcga ttctccgttg ccacgtgcct gattttgttg ctataatcgc
     6181 cggtttcgaa gctcgctcaa ttttcggacg gggttcaggc acgcagtacg cgagcatcgc
     6241 tcaaaccgta ttgaggcgcc gaccaaacct cgaaacacac cggaacacgg gcccgccttc
     6301 gcgtagtccg atcgattttt tcgactcatt tcgacacttt tgcgctcttg ggcaatcgct
     6361 tccaggagtt cgatctaaaa gcagcaccta ccacttgaag gtcaggtact ggaaacagga
     6421 acaggataag gaaccgtcat ggctaacacc gcacaagcac gtaagcgcgc ccgccaggcc
     6481 gccaaggcaa actcgcacaa ctcggcactg cgctcgaagt tccgcacggc tatcaaggct
     6541 gttcgcaagg caatcgaagc cggtgacaag gctaaggccg ctgagatctt ccaggcatcg
     6601 tcgaagacca tcgacatcat tgccgacaag aaaatcgttc acaagaacaa ggcagctcgc
     6661 aacaagagcc gtctggctgc agccctcaag ggtctgcaag cgcccgcagc gcagtaaatt
     6721 cggtcagccc gcttcggtgg gctacctcct gtttccgctg caacggaaaa agctcgcttt
     6781 ggcgggcttt tttgctttga actgacaggt ctgtaccaca ctctgtgact tgtcgcggcg
     6841 cgctcgcggt tgacgaatta cccgctgaag ccacgacctc agcccaaaaa aacccgctgc
     6901 tcgcgggttt tttcttttgc cgttccggct gacttacgct atatcacgtg ccttttggct
     6961 agcggcgtgg cgtgctcaag ctgcaattcg cacgcctcgg tcacgagaag gtcattgtct
     7021 ttcgcaaagt tcaggacgaa atcgaaggcc atcggctcga cgtcgcgcaa gcgcgaatcg
     7081 agaatcacgc atttaaggtc gccgatcatg gtgggccgga cgtacaacga gtattgcagg
     7141 taggcattcg gccccttgcc acgcgggcta ggaccaaagc acgccatcac acccgcgagc
     7201 cgctccgacc agtcgctcgg ccgaaatttt ttcccggtcg acgtgatgcc ctgaatgaaa
     7261 tattcggttg gagctgcttc ggccatgtag gaatacctat gtgactgccc agcgggacgg
     7321 ctgcaaagcg gactgcaaag tggctttggg atggctggac acgtcggccg cgcctgaatg
     7381 gcgagctgcg ccgccagaaa ccgcgaaggg ccggacaagc cgcctcggcg ctcatcatga
     7441 cggcgcaccg cgaggaaact ttccggagcg cgcgagccgt gtcccgcaag ctcaataccg
     7501 gcctgctggg cttagggatc accggtagat tatagcgcag cggacctttt tccgcagcgc
     7561 acacaagctc cgccgtaagg ttacaggagg ccttcaaagc gctttcccgg ccttttggcc
     7621 gactcgtcaa acggggcgaa atcctttatg ctgcattgag ttaccacaaa cgacggcggc
     7681 gccgtccggc aatcctgccg ggtgagaccg ccgtatttgt ttcatgaccg ccaagaaaat
     7741 tcgccactac ctgcagttca aggatttctc gctg
//
LOCUS       DATASH010000987         3349 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6299046,
            whole genome shotgun sequence.
ACCESSION   DATASH010000987 DATASH010000000
VERSION     DATASH010000987.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6299046"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..444
                     /locus_tag="VHV99_26315"
     CDS             169..444
                     /locus_tag="VHV99_26315"
                     /inference="COORDINATES: protein motif:HMM:TIGR01727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX3638933.1"
                     /translation="MVAHQGPTSELFARPRHPYTRALLDAAPSTEPEIERSRHVPLLS
                     GDPPSPSSPPAGCRFHTHSAFSEPVCEIAEPILRLVDEHRRVACHLA"
     gene            complement(494..1252)
                     /locus_tag="VHV99_26320"
     CDS             complement(494..1252)
                     /locus_tag="VHV99_26320"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX3638934.1"
                     /translation="MSNVGRRGNTALLDQPKTRIAVATAVAFGLWCAALVLIGGLLVT
                     PEITPPSPPNLDAQLVEIEPPPPRISSPAESAPAHPAHLAPPANVKHATPPTVHHATP
                     VQEEPSQHAAVPVAPPAAPSPVAAPSAAPSPAPKAPPVSATPSASTTNRSPAATDLAA
                     NGSGHTGARAITQPLPEIPDDLREAAYHAIALARFTIHPDGSADVELLQPTQQPRLNQ
                     LLLSALHNWRFFPATENGKPVESHQDVRVHLVVQ"
     gene            complement(1245..1643)
                     /locus_tag="VHV99_26325"
     CDS             complement(1245..1643)
                     /locus_tag="VHV99_26325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX3638935.1"
                     /translation="MKLKRSRQARRGKIEIIPMIDVMFFLLATFMLASLSMQRLDSMK
                     IDLPGGQAQRLNLDTPLTLTVTRANAIFVNRQPVPLAALAGDLRPLLKPDGNLVVAAD
                     DAASHGVVVKAMLEARKAGVQHFLIAVHHE"
     gene            complement(1640..2365)
                     /locus_tag="VHV99_26330"
     CDS             complement(1640..2365)
                     /locus_tag="VHV99_26330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX3638936.1"
                     /translation="MNDFSELFLAVRLGGWVIYPLTVLAVAGLAILLDKAYSLHRFAR
                     IPARIDQLIEAPVVDFAQTRAQIAALPERHALRSFAAPILDGGSAPIWWIESRAETAA
                     QHIERALNRGLWLLETIVTAAPLLGLLGTIIGMMHSFRLIGGQGVVNPTGVTGGVAQA
                     LIATAIGLVIALVALFAFNYFSRRVGELLDHFERVGTRLIGQLRLDQEDPEAEAQRRL
                     DRERAEGPAHLRLAREHAPGPRT"
     gene            complement(2412..>3349)
                     /locus_tag="VHV99_26335"
     CDS             complement(2412..>3349)
                     /locus_tag="VHV99_26335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX3638937.1"
                     /translation="SKTIGRLFGVYVQDEWKVNDKLIINYGLRYDKMNEVVDASQLSP
                     RIGLVYKATPSTTLHAGYARYFTPPPLELIAPTTVAQFNNTTNQAANPQNSPVQPERS
                     DYFDVGVTQRVNSAITVGVDAYYKKAKDLLDEGQFGTALINTPFNYQTGHDYGVEFTA
                     NYKDEHFSAYANLALSRATGENIISGQFNFDPAELAYIASHPVNLDHDQLVTGSAGIA
                     YTFARTTYSADTTLGSGLRSGFANTDHVPFYVNVNLAVIHHFQAPLIGNFDGRLVVVN
                     LLNRTYELRDGSGIGVGAPQFAPTRAFYAGITKYF"
BASE COUNT          585 a          980 c         1130 g          654 t
ORIGIN      
        1 cctgatggct atctgttgcg atttgcccaa gatcgcccac taacggagga cctcacgtga
       61 ccctccgccg tcgtaggcag gcaaacctta tttcgtacgc taacgacaag gttgtcagat
      121 ctggatttac accggtggcg acaatatgtc agctgagcgg acttcccaat tgtcgcgcac
      181 caaggcccta catctgagct ctttgcgcgg ccgcgccatc cctatacgcg tgccctgctg
      241 gatgcagccc cgtcgaccga acccgagatt gaacgttcgc gtcatgtgcc attactttcg
      301 ggtgacccac cgagccccag ttcgccgccg gcgggctgcc ggtttcacac acacagcgcc
      361 ttttcggagc cggtctgtga aatcgcggag ccaatcctta gactggttga tgaacaccgc
      421 cgagtggcct gtcacctagc ttagtaactt tgtccgtacc tagatgttca gcctatagca
      481 acaagttgtt cggctactgg accacgaggt ggacgcgaac gtcttgatgg ctctcgaccg
      541 gcttgccgtt ctcggtggcg gggaaaaaac gccagttgtg cagcgccgag agcagcaact
      601 ggttcagacg cggctgttgc gtcggctgca ggagttcgac gtcggcggag ccgtccgggt
      661 gaatcgtgaa gcgcgcaagc gcaatggcgt gatacgcggc ttcgcgcaag tcgtccggga
      721 tttccggcag tggctgcgtg attgcgcgtg cgccagtatg gccgctgccg ttggcggcga
      781 gatcggtcgc ggccggtgaa cgattggtgg tgctggcgga tggcgtggcc gataccggcg
      841 gcgccttagg tgcgggggat ggcgcagcgg aaggcgccgc aacgggcgag ggtgcagcag
      901 gcggcgccac aggcacggca gcgtgctggg acggttcttc ctgcacagga gttgcgtgat
      961 gcaccgtcgg cggtgttgcg tgcttaacgt tcgccggggg tgccagatgc gccggatgtg
     1021 ccggcgcgga ctccgcgggg gagcttatgc gcggcggcgg gggctctatt tcaacgagtt
     1081 gcgcgtcgag gttcggggga gacggcggcg tgatctcggg cgtgacgagc agtccgccga
     1141 tgagcacgag cgctgcgcac cacaacccga acgcgacggc cgtcgcaacg gcgatgcgtg
     1201 ttttcggttg gtccagcaag gccgtgttgc cgcggcggcc aacgttactc atgatgaacg
     1261 gcgatcagga agtgctgtac gccggctttc cgggcttcga gcatcgcctt gacgaccacg
     1321 ccgtgcgacg ccgcgtcgtc ggcggcaacc accaggttgc cgtccggttt cagcaacgga
     1381 cgcaggtctc ccgctagcgc cgcgagcggc actggctggc ggttgacgaa gatggcattg
     1441 gcgcgcgtga cggtgagggt gagcggtgtg tccagattga gccgttgcgc ttggccgccc
     1501 ggcaagtcga ttttcatcga gtcgagccgc tgcatcgaaa gcgatgcgag catgaacgtt
     1561 gccagcaaaa agaacatcac gtcgatcatc gggatgattt cgatcttccc gcgccgagcc
     1621 tgtcttgagc gtttcagttt cacgtgcgcg gccccggcgc atgctcacgg gcgagccgca
     1681 agtgcgccgg cccttccgcg cgctcccggt cgagccggcg ttgcgcctct gcttccggat
     1741 cttcctggtc gagtcggagc tgcccgatca gccgtgtgcc gacccgctcg aagtgatcga
     1801 gcagctcgcc gacccgccgg gagaaataat tgaacgcgaa gagcgcgaca agagcgatca
     1861 ccaggccgat cgcggtggcg atcagcgctt gcgcgacacc gcccgttaca ccggtcgggt
     1921 tcacgacgcc ttgtccgccg atcagccgga acgagtgcat catgccgatg atcgtgccga
     1981 gtagtcccag cagaggcgct gctgtcacga tggtttccag cagccacagc ccgcggttca
     2041 gcgcgcgctc gatatgctgc gcggcggtct cggcgcgtga ctcgatccac cagatcggcg
     2101 cactgccgcc gtcgaggatc ggcgcggcga agctgcgcag cgcatggcgc tcgggcagcg
     2161 ccgcaatctg tgcgcgtgtc tgcgcgaaat cgacgacggg ggcttcgatc agttggtcga
     2221 tgcgggccgg aattcgcgcg aaccggtgca gcgaatacgc cttgtcgagg aggatcgcaa
     2281 ggccggccac cgcgaggacg gtcaaggggt agatgaccca tccgcccagg cgcacggcga
     2341 gaaacagttc ggaaaaatcg ttcatggtgt cgtgacttcc tgatcatgcg gcggcatgcc
     2401 gcgcaggcgg tttaaaagta tttcgtgatg cctgcgtaga aggcgcgcgt cggtgcgaac
     2461 tgcggggcgc caacgccaat tccggaaccg tcgcgcaatt cgtaggtgcg attgagcaga
     2521 ttgaccacga ccaggcgtcc atcgaagttg ccgatcaggg gcgcctggaa gtgatggatg
     2581 actgcgaggt tgacatttac atagaacggc acatggtccg tgttcgcaaa tccgctgcgc
     2641 agaccgctgc ccaaggtggt gtccgcgctg taggtcgtgc gcgcaaacgt gtaggcgatg
     2701 cccgccgaac cggtgaccaa ctggtcatgg tcgaggttga cgggatggct ggcgatgtac
     2761 gcaagttccg cgggatcgaa attgaactgg cctgagatga tgttctcgcc agttgcgcgg
     2821 ctgagcgcca gattcgcgta cgcggaaaaa tgctcatcct tgtagttggc ggtgaattcg
     2881 acgccgtagt cgtggccggt ctggtagttg aacggcgtgt tgatcagggc ggtgccgaac
     2941 tggccttcgt cgagcagatc cttcgccttc ttgtagtaag catccacgcc taccgtgatg
     3001 gcggagttaa cgcgctgggt tacgccgaca tcgaagtagt cgctgcgctc gggttgcacc
     3061 ggcgaatttt gcggattcgc ggcctggttc gtcgtgttgt tgaactgcgc caccgtggtc
     3121 ggcgcgatca gttcgagagg gggaggcgtg aagtaccgcg cgtaacctgc atgcagcgtg
     3181 gtgctcggcg tcgccttgta caccaggccg atgcgcgggc tcaactgact ggcgtcgacg
     3241 acttcgttca ttttgtcgta gcgcagcccg taattaatga tgagcttgtc gttcaccttc
     3301 cactcgtctt gcacatagac gccgaagagc cggccgatcg tcttgctcg
//
LOCUS       DATASH010000988        11759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6496610,
            whole genome shotgun sequence.
ACCESSION   DATASH010000988 DATASH010000000
VERSION     DATASH010000988.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 11759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11759
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6496610"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(361..1422)
                     /locus_tag="VHV99_26340"
     CDS             complement(361..1422)
                     /locus_tag="VHV99_26340"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX3638938.1"
                     /translation="MQPVNLQHAVQFAIDHETPWDREVDGVWGVHSDDPPPWNRLLGP
                     VHGRGPVSGVITRAGRPLTAWGEPDRADLTFSIAKTYLALLAGVAHDRGLMPDVDEPV
                     HVRVPGIGFDDEHNASVSWTHLLQQTSEWQGVCFGLPDEADHYRYVRFGKEPDGKKGQ
                     RRPLRVPGTYWEYNDVRINQFSLALLHLFRRPLPEVFREAIMRPLDASENWQWVGYDN
                     AWVEVGGQRVQSVPGGTHWGGGLSISAQDQIKVAQLLLGQGSVNGQQIVSSEWLQRML
                     TPCAIAPFYGCLVWLNHERSVFPSVPASSYFAIGAGGSFTWIEPERELAVVVRWIDPA
                     HADEFFGLVLKAVDEGATG"
     gene            complement(1432..2583)
                     /locus_tag="VHV99_26345"
     CDS             complement(1432..2583)
                     /locus_tag="VHV99_26345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HEX3638939.1"
                     /translation="MDSDLIDMMMKWRHALHSCPETAFEEVATADLVAGVLRQHGFEV
                     QGGIGRTGVVGSMTRSAGPAIGLRADMDALPIVEANSFAHRSQHEGKMHACGHDGHTA
                     MLLGAAVALSRDPGWRGTVHVIFQPAEEGPAGGGRMMVEDGLFERFACDAVFGLHNWP
                     GIPRGHFAINDGPMMASFDTFEIVVDGNGSHAAMPEEGSDVLVCASQIVTALQTIISR
                     RISPKESGVVSVTQIHGGEAWNVLPDRAVIRGTVRCFSSDVQERIRASIDEISTGIAR
                     ALGTNATLDYRYAYPATINTAAQADLAAAAAGATVGVASVQRGCVPSMASEDFAFMLH
                     AKPGAYIWMGVDEATPAAPLHNPHYDFNDAALAIGATYWVNLVKLFCAR"
     gene            complement(2709..4001)
                     /gene="oxlT"
                     /locus_tag="VHV99_26350"
     CDS             complement(2709..4001)
                     /gene="oxlT"
                     /locus_tag="VHV99_26350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate/formate MFS antiporter"
                     /protein_id="HEX3638940.1"
                     /translation="MSTQDFGARSRAPLALSLSLQSRWWQLLCGLVCAMVASNPQYIW
                     TLLTRPLTAQLGVSLPSLQVTFSILIVCQTFLSPLEGYLIERFGPRWLLAAGGAITGL
                     SWVLTSRAESLLSVYLCYGVLGGIGVGIVVVGTIGLMARWFPDRRGFAIGVVMAGFGM
                     GAMLTTFPITNSIAKYGYQHTLVVFGVILGVVGILAALGMRLPPPGYMADWRPQKAAA
                     AAPDTRPGSMLRTPLFWLMFVMMSMMATSGLMVTSQMAVFTRDFGMSGVTVFGLAALP
                     LALTIDRVANGVTRPMFGWISDLIGREHTMLIAFGMEAIAMTLWLTSRHDPLLFVLLS
                     GVVFLGWGEIFSLFPSTLTDTFGGKHAIVNYACLSYAQGVGSILGGPVAALLHEHTGG
                     WDAVFGTAIALDSCTAVLAIAALRPMRRAWFARTSSRS"
     gene            complement(4699..5712)
                     /locus_tag="VHV99_26355"
     CDS             complement(4699..5712)
                     /locus_tag="VHV99_26355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX3638941.1"
                     /translation="MEDYFVFNAGNVVLQSGITFRDAKLAYQTYGKLNADKSNVILYM
                     TSFSAHHYDIEWMVGPGKALDTNKYFVVIPNLFGNGLSSSPSNATEPFNGSRWPNFTI
                     ADNVAIQHQLLTQELGIEVLQMACGWSMGGLQAYQWASMYPDMVRRLVVLCGAAKASP
                     HNQVFIDGVRATLTTDAAFQNGMFVERPERGLRAMGRNYAAMAMSQTFYRNEVWRQAG
                     FSSLEDYLVMSWEANFLKRDGNNLLAHLWTWQHANIAANSTYNGDFHAALKAITAKAL
                     IMPSTTDLYFQVEDNRLEVEQMRNATLMPISSIWGHRAGLPTANVEDAAFITAAITRH
                     LED"
     gene            complement(5946..7604)
                     /locus_tag="VHV99_26360"
     CDS             complement(5946..7604)
                     /locus_tag="VHV99_26360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3638942.1"
                     /translation="MRDFDFKTAAPSAAGPATTVLAVNDLTISLPAGADRPHAIRHVS
                     LDVRQGEIVCLLGESGSGKSVIAQAVMGLLPASLKALDGSIELLGEDVLTASPDRIRQ
                     LRGEKMAMVFQEPMTALNPIMTCGAQVDEMLAQHSRAGVGERRRRILEIFAHVRLPEP
                     ERIYASYPHQLSGGQRQRIVIAMALILKPSLLICDEPTTALDVTTQAEILKLIRELQT
                     ESGTAVLFITHDFGVVAEIADRVVVLRLGDMVEQGDKLQVLQQPREPYTRMLLAAVPA
                     LVPRTRPPVEKAYPLLDARGIGKTYVTGSWPGRKRKVRAASDVSLMVHPGETVGIVGE
                     SGSGKSTVARCIARLIEPTAGDIFADGRSVAKARGRALYPFRRGVQVVFQDPYRSLDP
                     RQTVGASIVEGPLNFGVPYAQAWQRAEELMTLVRLRPDALNRYPSEFSGGQRQRISIA
                     RALACEPQVLIADEAVSALDVSVQAQILDLLDDIQQRLRIGILFITHDLRVASQICDR
                     VIVMRRGEIVEEGAIQDVFLAPRHEYTQALLAAAPGRGFAFGAA"
     gene            complement(7609..8526)
                     /locus_tag="VHV99_26365"
     CDS             complement(7609..8526)
                     /locus_tag="VHV99_26365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3638943.1"
                     /translation="MLANLEVAPAVRSRTPLWRRVLRNWSVRLGCGALLLLLFVAVAA
                     PWLGTIDPTAMDPASANLAPGVTAEFSDLSGATFQHFFLMGTDSLGRDTWSRVVYGAR
                     VSLSVGITVALLALTFGALVGVAAGYFRRLDDVIMRAMDSLMAIPSILFAITLMALWR
                     PTLVTVIVAITIPEIPRVARLVRSIVLTIREEPYIEAAISLATPTVKILWRHILPNAI
                     APLIVQGTYVCASAILVEAILSFLGVGLPPDIPTWGNVMADGRMQFNQYPQTVLFPGV
                     FLALTVLAVNILGDGLRDTLDPKFNKRGS"
     gene            complement(8589..9530)
                     /locus_tag="VHV99_26370"
     CDS             complement(8589..9530)
                     /locus_tag="VHV99_26370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3638944.1"
                     /translation="MAFFLFRRVLSTAPVLVVVALIVFMILRLTPGDPAAALVGDNGT
                     SADIARIHASLGLDRPLPVQFVKWTGQLLQGNLGDSYYMKQPVTRLIAQRIAPTVSLS
                     ALTLVMTLLLAVPLGVIAASRHGGWLDRMLMGVSVMGFSVPSFVVGYILIWAVALHAK
                     LLPVQGYAPLSAGIGPWLTHLILPAITLSIVYLALIARVTRAAVAEALTEDYIRTARA
                     KGISEARVLIRHALANAAVPIITVIGIGVGLLIGGVVVTETVYAIPGLGQLTVDAVLA
                     RDFPLVQGITLFFSFVYVLINLLVDMSYLLLDPRIRY"
     gene            complement(9627..11207)
                     /locus_tag="VHV99_26375"
     CDS             complement(9627..11207)
                     /locus_tag="VHV99_26375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3638945.1"
                     /translation="MLKRLFTQCVAGALIASAATMVSAQDESKVLRIVPQADLKILDP
                     IWTTAFITRDHGYMIYDTLFGVDANGVVKPEMVDTYTTSPDGKTWTFTLRKGLAFSDG
                     KPVTSADVIASITRWGKRDVFGERMFTALQSFDAVDAKSFRMTFKQPFPMVLDALGKP
                     SGSAAFIMPKRVADTPADQQISDYTGSGPYIFKQDEYRPGAKIVYIKNPNYVPRPEPA
                     SGTAGGKHVYVDRVEWIVLKDAQTQANAIANGEVDMIEWMPAEQYAAFKSNPKLELVK
                     PTPAGLFDLHLNHLVAPFNNPKIAQAAIMAINQEALMRAQLVNKDLYRTCSSIYPCDS
                     PLASTDTAYFTGKPQFAAARNLLKEAGYDGKPVVLLYPSDYAVLNKFPPVMAQLLKQA
                     GFNVDLQAMDWSTLVTRRTSKAAADKGGWNLFITGWAPGDTMNPLFFTPLTGNGEKGW
                     FGWATDDKVEQLKNEYLASTDAAQRKKIATAIQLETYQSGILAPLGDFDFYSVIRKGV
                     VSGVVPAPVNVFWNIRKS"
     gene            complement(11488..>11759)
                     /locus_tag="VHV99_26380"
     CDS             complement(11488..>11759)
                     /locus_tag="VHV99_26380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638946.1"
                     /translation="RNVPFACVKNRYLGRTLNSVQKSPLRVSHAALAPGIGATTILYC
                     VMHRPRAHLHNARALQRVLAGGAIARLPHTYWISRMMARFVPAKV"
BASE COUNT         2442 a         3702 c         3503 g         2112 t
ORIGIN      
        1 ggctgcatat cgacgaaacg cagcacgcgc aggtcgcgaa gcgttacgtc acattcggct
       61 tcgtaaaggc cagacgccac gtccttggcc aacttgctca gtacatcgag gcggaagttt
      121 aaatgttcca gtcttggata cggcgccttc gacatgctgt tctcccattc agctactctg
      181 attcagttgc acgctgcaac ttttaaattt tacgtgtttc gcgcacgaaa catgccatcg
      241 tgcggaaacc cccatacgtg cagacaagcc gggggctcgc caaacggcat gagagtaccg
      301 caacgcgcac gaatggggca agcgcgcacg atgccggtgc ggcaagtgga tggacgctga
      361 ttaacccgtc gccccctcgt cgaccgcctt gagcaccaaa ccgaaaaact cgtccgcatg
      421 cgccgggtcg atccagcgca ccaccaccgc cagctcgcgt tcaggttcga tccaggtgaa
      481 cgaaccaccc gcgccaattg caaagtagct ggacgccggc acgctcggaa agacgctacg
      541 ctcgtggttg agccagacca ggcagccata gaacggtgcg atcgcacacg gtgtcagcat
      601 gcgctggagc cactcgctgg aaacgatctg ctgcccattg acgctgccct gacccagcag
      661 cagctgagcc accttgatct ggtcctgggc gctgatcgac aggccgccgc cccaatgcgt
      721 gccgcccgga accgactgca cgcgctgccc gcccacctcc acccaggcat tgtcgtaacc
      781 gacccattgc cagttctcgc tcgcgtcgag tggccgcatg atcgcttcgc gaaacacttc
      841 cggcaacggg cggcggaaca gatgcagcag cgccagcgaa aactggttga tgcgcacgtc
      901 gttgtattcc cagtaggtgc cgggaacccg taacggccgc cgctgccctt tcttgccatc
      961 gggctccttg ccgaatctga cgtaccggta gtggtcggcc tcgtcgggca agccgaaaca
     1021 cacaccttgc cattcgctcg tctgctgcag cagatgcgtc cagctcacgc ttgcattgtg
     1081 ttcgtcatca aagccgatac ccggcacgcg cacgtgcact ggctcgtcga catcgggcat
     1141 caggccgcga tcgtgcgcaa cgccggccag cagcgcgaga taagtcttgg cgatgctaaa
     1201 ggtcaggtcg gcacgatccg gttcgcccca cgcagtgagc gggcggccgg cgcgagtgat
     1261 gacgccagac accgggccac gcccatgcac cggtccgagc agccggttcc agggtggcgg
     1321 gtcgtccgaa tgtacgcccc atacgccatc gacctcgcgg tcccagggcg tttcatgatc
     1381 gatcgcgaat tgcacagcat gctggagatt gacaggttgc atgagggtcc tttaccgggc
     1441 acaaaacagc ttgacgagat tcacccaata agtcgcgccg attgcaagcg cggcatcgtt
     1501 gaaatcgtag tgaggattat ggagcggtgc tgccggagtc gcctcgtcaa cacccatcca
     1561 gatgtacgcg ccgggttttg catgcaacat gaacgcaaaa tcttccgagg ccatcgaggg
     1621 cacacaacct cgctgcacgc tggcaacacc gacggtcgcg ccggcggccg cagctgcaag
     1681 atcggcttga gcggcagtgt tgatggtggc cggatacgcg tagcgatagt cgagcgtagc
     1741 gtttgtgccc agtgcgcgcg caatacccgt ggagatttcg tcgatcgagg cgcggattcg
     1801 ttcctgcacg tcgctcgaga agcagcgcac ggtgccgcga atgaccgcac ggtcaggcag
     1861 cacgttccac gcttctccac catgaatttg cgtgacgctc acgacgcctg attctttcgg
     1921 cgaaatccgc cggcttatga ttgtctgcaa tgcggtgacg atctggctgg cacacaccag
     1981 cacgtcgctc ccctcctccg gcatcgcggc atgcgaaccg ttgccgtcga caacaatctc
     2041 gaacgtgtcg aacgacgcca tcatcggacc gtcgttgatc gcaaaatgtc cgcgcgggat
     2101 gccgggccag ttgtgcaaac caaacacggc gtcgcacgca aagcgctcga agagtccgtc
     2161 ttcgaccatc atcctgccgc cgcccgccgg cccttcttct gctggctgaa aaatcacatg
     2221 taccgtgccg cgccagccgg ggtcgcggct caacgcgaca gcggcgccga gcagcattgc
     2281 tgtatggccg tcatggccac acgcatgcat cttgccttca tgttgcgacc gatgcgcgaa
     2341 gctattggct tcaacgatcg gcagcgcgtc catgtcggcg cgcaagccga tggccggtcc
     2401 cgcgctgcgg gtcatgctgc ccaccacacc ggttcgtcca atgccgccct gtacctcgaa
     2461 gccatgctgt cgcaatacgc cagcgacgag atcggcggtt gcgacctcct cgaacgcagt
     2521 ttccggacat gagtgcagtg cgtgccgcca cttcatcatc atgtcgatca ggtcgctatc
     2581 catcggaatt caatcccttt cagtggaaaa ttgaggagac aaaaaaagca gagaatcgac
     2641 gaagcggcac agcatccgcg cagattcgaa tgggcgagat tgctggtaag cggccgtggc
     2701 ccgttgcatt acgagcgact ggatgtgcgg gcaaaccacg cgcggcgcat cggcctgagt
     2761 gcggcgatcg cgagcacagc ggtacaacta tccaacgcga tagccgtgcc gaacaccgcg
     2821 tcccaaccac ccgtatgctc gtggagtagc gcggccaccg ggccgcccag gatcgagccg
     2881 acgccttgcg cataagacag gcacgcatag ttgacgattg catgcttgcc gccaaaggtg
     2941 tccgtgagcg tcgaagggaa aagcgaaaag atctcgcccc agccgagaaa gacgacgcct
     3001 gacaacagca cgaacaacaa cgggtcgtgc cgcgacgtga gccacaaggt catcgctatc
     3061 gcttccatcc cgaaggcgat cagcatcgta tgctcgcgcc cgatcaggtc ggagatccac
     3121 ccgaacatag gccgcgtcac gccgttcgcc acgcgatcaa tggtcaacgc aagcggcaac
     3181 gccgcgaggc cgaacacggt gactcccgac atgccgaagt ctcgcgtgaa gactgccatt
     3241 tgtgaggtca ccatcaggcc ggaggttgcc atcatcgaca tcatcacgaa catcagccag
     3301 aacagcggcg tgcgcagcat gctgccgggc cgcgtgtcgg gtgcggcagc ggcggctttc
     3361 tgcggccgcc agtccgccat atagccgggc ggcggcagcc gcatgccgag cgcggcaagt
     3421 atgccgacca caccgaggat cacgccaaag acgaccagcg tatgctgata gccgtatttg
     3481 gcgatgctat tggtgatcgg aaacgtcgtg agcattgcac ccatgccgaa gcccgccatc
     3541 accacgccaa tcgcaaaacc gcgacggtct gggaaccagc gcgccatcag cccgatcgtg
     3601 ccgaccacga cgatacctac gccaatcccc ccgagaacgc catagcagag atagacggac
     3661 agcaaagact cggcgcggct cgtcagtacc cagctaagcc ccgtgatcgc gccgccggcg
     3721 gccagcagcc agcgcggccc aaagcgttcg attaaatagc cttcgagcgg cgacagaaaa
     3781 gtctggcaga caatgagaat ggagaaggta acctgcagac tcggcagcga cacgccgagt
     3841 tgcgctgtca gcgggcgcgt cagcaacgtc cagatgtatt ggggattaga ggcgaccatg
     3901 gcacatacga ggccacacag caattgccac cagcggctct gtagtgaaag tgaaagtgca
     3961 agtggcgcgc ggctgcgggc gccaaaatcc tgggtgctca tgcttcaact ccgtacgaat
     4021 aagtgacacg aaatgacttg ggcagtgcgc ttgcctggag gcctgaaacg acgctcctgg
     4081 acaacgcgct cagtgcatcg gagcgtaagt tcagatgacc cagccttgaa aacggcacct
     4141 tcgacgatct gccggtgtgt aggcatcccg ccgatatagt tgcaatttcc aataattgcc
     4201 attcacatgt gactgcgatt tgcacgaagt gtgccacgac gcgggaaacc ccgtgttcat
     4261 ggacaaatca ggtgcttttc atactgcgac gcacgcgtga ccatgcgcga gaatcatgcg
     4321 ccgagcgcgc gagaaaggtg cactgcgcga ctgaaattca gtgttgcacc gattccgggc
     4381 gtaaccttgg cattcgtcga gcctgtacgg ttgcgacgat ccaggggagt ggttcaaatg
     4441 tgacgacgca gtctgccgaa acaccccgca gttcgtcgcg gtgcgcacgg gaggtcgtgc
     4501 gacctggcgt cagggttcgg tggcccggat gattatcatg ccggggacgc ctgtggcgtc
     4561 cccggcatgt tgcgctcgcg cgctcatctg catcagcgac ttccgcaatg ttgcccgaca
     4621 gcatcggcgt ggtggcttgc ggtgccgaag aaggactgca ggaaaagcac gagacgccgc
     4681 cgcggcatgc ccagcgcgtc aatcctcaag atgccgggtt atcgcagcgg taataaacgc
     4741 ggcatcttcc acattggctg tcggcagccc ggcgcgatga ccccatatcg acgaaatggg
     4801 catgagcgtg gcgttgcgca tctgctcgac ttcgagacgg ttgtcttcca cctgaaaata
     4861 cagatcggtt gtgctcggca tgatcaacgc ctttgccgta atcgctttca gtgccgcgtg
     4921 aaaatcgccg ttgtaagtgg aattagcggc gatgttcgca tgctgccagg tccatagatg
     4981 agccagcagg ttgttgccgt cgcgcttcag gaaattcgct tcccacgaca tcacgagata
     5041 gtcttcgagc gaggaaaagc cggcctgccg ccacacttcg ttacgataaa acgtctgcga
     5101 catcgccatc gcggcgtagt tgcgacccat ggcccgcaga ccgcgttcgg gccgctcaac
     5161 gaacattccg ttttgaaagg ccgcatcagt cgtcagggtc gcgcgcacgc catcgatgaa
     5221 aacctgattg tgcggcgagg cctttgccgc gccgcagagc acaaccagcc gcctgaccat
     5281 gtccggatac attgatgccc attgatacgc ctgcaacccg cccatcgacc acccgcatgc
     5341 catttgcaga acttcgatgc cgagttcttg cgtcaggagc tggtgttgaa tcgcgacgtt
     5401 atcggcaatc gtaaaattcg gccaacggct cccgttgaac ggctcggttg cgttgctggg
     5461 cgaggacgac agtccattgc cgaacagatt cggaataacg acaaagtact tgttagtgtc
     5521 tagcgccttc cccggaccga ccatccactc gatgtcatag tgatgcgcgg aaaacgacgt
     5581 catatacaga atgacattcg atttatctgc gttcagcttt ccgtaggttt gataggcgag
     5641 cttggcatct ctgaaggtga taccggattg caacaccacg tttccggcat tgaatacgaa
     5701 atagtcttcc attgataggg ggtccctctt ggtcaaatgt ggcgaatggt aatggaaaaa
     5761 tcagatagac gtctcgatgc gccgccgtgg catgaagctc ggccatacac gcttcacgca
     5821 cgcgggagcg atcttcacca gcagttcagc agtgaacgcg tcccacagca ggtagtggat
     5881 agcatgagtg ccagcggcgc cgctgaccgg aaactcatga aatcactgtg atcaaagggc
     5941 ccgcctcaag cggcgccgaa cgcaaagccg cgccctggcg ccgccgccag caaggcctgc
     6001 gtatattcgt ggcgcggcgc gaggaacacg tcctggatcg ccccttcctc gacaatctcg
     6061 ccgcgccgca tcacgatgac ccgatcgcag atctggctgg ccacccgcag atcgtgcgta
     6121 atgaacagaa taccgatccg cagccgctgc tgaatgtcat ccagcaggtc gagaatctgc
     6181 gcttggaccg agacgtcgag tgcggacacc gcctcgtcgg caatcaacac ttgtggctcg
     6241 catgccagcg cccgcgcgat cgaaatgcgt tgccgctggc ccccggagaa ttcgctaggg
     6301 tagcggttca gcgcatcggg tctgagccgt accagcgtca tcaattcttc agcacgttgc
     6361 cacgcttgag cgtacggcac gccgaaattc aacggccctt cgacgatcga ggcgcccact
     6421 gtctggcgcg gatccagcga ccggtagggg tcctggaaca cgacctgtac gccgcgtcga
     6481 aacggatata gcgcgcggcc gcgcgctttg gccaccgatc ggccgtcggc gaagatatcg
     6541 cccgcagtcg gctcgatcag ccgcgcaatg cagcgcgcga cagtcgattt tcccgagccg
     6601 gactcgccca cgattccgac cgtttcgccc ggatgcacca tcagcgaaac atcactggcc
     6661 gcccgcacct tgcgcttgcg gcccggccag gagcccgtca catacgtctt cccgatcccg
     6721 cgcgcgtcga gcagcggata ggctttctcc actggtgggc gcgtgcgcgg caccagcgcg
     6781 gggaccgctg ccagcagcat acgggtatag ggctcgcgcg gctgctgcaa tacttgcaac
     6841 ttgtcgccct gctcgaccat gtcgcccaac cgcaacacca cgacacggtc agcaatctcg
     6901 gcgacaacgc cgaaatcgtg cgtgatgaag agcacggcgg ttccgctctc cgtctgcaat
     6961 tcacgaatca gcttgaggat ctcagcctgg gtcgtgacgt cgagcgccgt ggtgggctcg
     7021 tcgcagatca gcagcgacgg cttcaggatc agcgccattg cgatcacgat gcgctggcgc
     7081 tgtccacccg acaactgatg cggataagac gcgtaaatac gctccggttc cggcagcctg
     7141 acgtgggcga agatctcgag gatgcgccgg cgccgctcgc cgacgccggc acggctatgc
     7201 tgtgccagca tctcgtcgac ctgcgcgccg cacgtcatga tcggattgag ggccgtcatc
     7261 ggctcctgga aaaccatcgc cattttttcg ccgcgcaatt ggcggatgcg atccgggctg
     7321 gctgtcagca cgtcctcgcc gagcagttcg atgctgccgt ccagcgcctt gagcgacgct
     7381 ggcagcaagc ccatcactgc ctgtgcgatc accgatttgc ccgagccaga ctcgcccagc
     7441 aggcagacaa tctccccctg ccgcacgtcg agcgagacgt ggcggatcgc atgggggcgg
     7501 tcggcaccgg ccggcaggga aatggtgagg tcattcacgg caaggacggt tgtggccggc
     7561 ccggcggcgg agggtgccgc cgttttgaag tcgaaatcgc gcatcgcgtc agctcccccg
     7621 tttgttgaat tttggatcga gcgtgtcacg caatccgtcg cccaggatat tgaccgccag
     7681 caccgtcagt gccagaaaaa caccagggaa cagcacggtc tgaggatact ggttgaactg
     7741 catgcggccg tcggccatga cgttgcccca cgtcggaata tccggtggca ggccgacgcc
     7801 gagaaacgac aggatcgctt ccacgaggat ggccgacgcg cacacgtagg ttccttgcac
     7861 gatcagcggc gcgattgcat tcggcagaat atgacgccac agaatcttga cggtcggcgt
     7921 ggccaacgaa atggccgcct cgatgtaggg ttcctcgcga atggtcagca cgatcgagcg
     7981 taccaaccgc gcgacgcgcg gaatttcggg aatcgtgatc gcgacaatca ccgtgaccag
     8041 cgtcggtcgc cagagcgcca tcagcgtaat cgcgaacagg atgctgggaa tcgccatcaa
     8101 gctgtccatg gcgcgcatga tgacgtcgtc gaggcggcga aaataccccg ccgccacgcc
     8161 gaccagtgcg ccgaaggtga gcgccagcag tgccaccgtg atgccgaccg acagggacac
     8221 gcgcgcaccg tataccaccc ggctccaggt atcccggccg agactgtcgg tgcccatcag
     8281 gaagaagtgc tggaacgtcg cgcccgacag atcactgaat tcggccgtca cgcccggcgc
     8341 gagattggcg ctcgcgggat ccatcgcggt cgggtcgatc gtgccaagcc acggtgcggc
     8401 gacggctacg aagagcagaa gcaacagtgc gccgcatccc agacgcaccg accaattccg
     8461 cagcactcgg cgccacaacg gtgtgcggga ccgtacagcc ggcgccacct ccaggttggc
     8521 aagcatggcg cccggcgcgg caagggtcat cggatcgtga tcgtcgctca tgtccaatcg
     8581 tccttcactc aatagcggat acgcgggtcg agcaacagat agctcatgtc gaccagcagg
     8641 ttgataagca cgtaaacaaa ggaaaagaac aacgtgatgc cctgtaccag cgggaagtcg
     8701 cgcgccagta ccgcgtcgac cgtcaattgc ccgagcccgg ggatcgcgta gacggtttcg
     8761 gtgaccacca cgccaccgat cagcaagcca acgccgatgc cgatcaccgt aatgatcggt
     8821 accgcggcat tggccagcgc atggcgaatc aacacgcgtg cttcggagat acctttggcg
     8881 cgggcagtgc ggatgtagtc ttcggtcagc gcctcggcaa ccgcggcgcg cgtcacgcgg
     8941 gcaatcagcg caaggtagac gatggacagc gtaatggcgg gcaggatcag gtgtgtgagc
     9001 cacggaccga tgcccgcgct cagcggcgcg tatccctgca ccggcagcag cttcgcatgc
     9061 aaggcgaccg cccatatgag gatatagccg accacgaacg acggcactga gaatcccatc
     9121 accgacacgc ccatcagcat ccggtccagc cagcctccgt gccgcgaggc ggcgatcacg
     9181 ccgagcggca ccgcgagcag gagtgtcatc accagcgtca gggcggacag cgagacggtc
     9241 ggtgcgatgc gttgcgcaat caaccgggtg accggctgtt tcatgtagta cgagtcgccg
     9301 aggttgccct gcagcaactg cccggtccat ttgacgaact gcaccggcag cggccggtcg
     9361 agcccgaggc tcgcgtggat ccgcgcgatg tcggcgctag tgccgttgtc accgacgagc
     9421 gccgcagccg gatcgcccgg cgtcaggcgc aaaatcatga atacgataag ggcgacaacg
     9481 accagcactg gcgccgtcga caatacgcgc cggaaaagaa agaaagccat gtcggttacg
     9541 ctccaaagcc ggttgacaga tggacgggct cgcgtttgct ctgcgagacg gcggccggcc
     9601 ggctaacagc ccgccgccga caccggttag ctcttgcgaa tgttccagaa cacgttgacc
     9661 ggagccggga ccacgccgct gaccacgccc ttgcggatca ccgaatagaa atcaaagtcg
     9721 cccaacggcg ccaggatccc gctctgatac gtttccagct ggatcgccgt agcgatcttc
     9781 tttcgctgcg cagcatcggt gctggccagg tattcgttct tgagctgctc gaccttgtcg
     9841 tcagtcgccc agccgaacca gcccttctcg ccattgcccg tcagcggcgt gaagaacaac
     9901 ggattcatcg tgtcgccggg tgcccagccg gtaatgaaca ggttccagcc acccttgtcg
     9961 gccgccgcct tgctggttcg gcgcgtcaca agcgtggacc agtccatcgc ctgaaggtcg
    10021 acattgaagc cggcctgctt caacagctgc gccatgactg gcggaaattt attcagtact
    10081 gcgtagtccg acggatacag caacaccacc ggcttgccgt cgtagcccgc ttccttcagc
    10141 agattgcggg ccgccgcgaa ctggggcttg ccagtgaaat aggcggtgtc ggtcgacgcc
    10201 agcggcgaat cgcatgggta aatcgacgaa cacgtccggt acagatcctt gttcaccagt
    10261 tgggcgcgca tcaacgcttc ctggttgatc gccatgattg ccgcctgagc aatcttcggg
    10321 ttgttgaacg gcgccacgag atgattcagg tgcaggtcga agagcccggc gggcgtcggc
    10381 ttcaccagct ccagcttggg attgctcttg aacgcggcgt actgctcggc gggcatccat
    10441 tcgatcatgt ccacttcacc gttcgcaatc gcattggcct gcgtctgcgc gtccttgagc
    10501 acgatccatt cgacccggtc gacatagaca tgcttgccgc ccgcggtacc ggaagccggc
    10561 tccggtcgcg gcacatagtt cgggttcttg atgtacacga tcttcgcgcc agggcgatac
    10621 tcgtcctgtt tgaagatgta ggggcccgac ccggtgtagt cggagatctg ctggtccgcc
    10681 ggcgtatcgg cgacccgctt cggcatgatg aacgcggcgc tgcccgacgg cttgccgagc
    10741 gcgtccagga ccatcgggaa gggttgcttg aaggtcatgc ggaaggactt ggcgtcgacc
    10801 gcatcgaacg actgcagggc cgtgaacatt ctttcaccga acacgtcgcg cttgccccag
    10861 cgcgtgatcg acgcgataac gtcagccgag gtgaccggct tgccgtcgct gaacgcgagc
    10921 cccttgcgca gcgtgaacgt ccaggtcttg ccgtccggcg aggtcgtata cgtgtcgacc
    10981 atctccggtt ttactacgcc attggcatcc acgccgaaca gtgtgtcgta gatcatgtag
    11041 ccgtgatcgc gcgtgatgaa tgccgtggtc cagatcggat cgaggatctt cagatcggct
    11101 tgcggcacga tccgtaacac cttggattca tcctgcgcag acaccatcgt cgcagcgctc
    11161 gcaatcagcg ccccagccac gcattgagta aacagtcgct tcaacatgct tcactccaag
    11221 aggaagaaat gacacaaaat gaattcgggc cgtgcttgcg tcgttattag cggccgagtt
    11281 cactcgcccc ctccttcgac gtgttcagta agcgtgttga gacagtggta cggcgcgtca
    11341 cgtccctgcc cggacgccgc actccacgcc cacgcgtcga gtgcatccag acggaacttc
    11401 agatgcttca atttcggtta tgcgcccgca acaggcagat ctccgatcca ccgtgctaca
    11461 ttaattgcaa cttgcaacaa ttgaaattca cactttcgca ggcacgaagc gtgccatcat
    11521 tcgggaaatc cagtacgtgt ggggaagtcg ggcgattgcc ccaccggcaa gcacgcgctg
    11581 cagtgcgcgg gcgttgtgca agtgcgcgcg aggacggtgc atcacgcagt acaagatcgt
    11641 cgtcgcaccg attccgggcg cgagagcggc atgactcacc cgtagcgggc ttttttggac
    11701 gctatttaaa gtacgaccta agtacctatt tttcacacac gcgaaaggaa cgtttcgag
//
LOCUS       DATASH010000989         3067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2741187,
            whole genome shotgun sequence.
ACCESSION   DATASH010000989 DATASH010000000
VERSION     DATASH010000989.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2741187"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1380)
                     /gene="gatA"
                     /locus_tag="VHV99_26385"
     CDS             complement(<1..1380)
                     /gene="gatA"
                     /locus_tag="VHV99_26385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006494379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEX3638947.1"
                     /translation="MHEKSLTELRAALAAKECSAVELAQLYLKRIDAANSLNAFIQVD
                     ADLTLAQAKAADALLHTGHAGPLVGLPIAHKDVFVTKGWRSTAGSKMLSNYASPFDAT
                     VVARLQNAGMVCVGKTNMDEFAMGSSNENSYFGPVQNPWDRKAVPGGSSGGSAAAVAA
                     RLAPAATGTDTGGSIRQPASFSGITGIKPTYGRVSRYGMIAFASSLDQGGPMAQSAAD
                     CATLLNAMAGFDERDSTSLTRENEDFTRYIGKTWKEDSADKPLAGLRIGLPKEYFGAG
                     LADDVRASIDAALKQYEALGATLVDVSLPKTELSIPVYYVIAPAEASSNLSRFDGVRF
                     GHRAAEYRDLLDMYKKSRAEGFGPEVKRRILVGAYVLSHGYYDAYYLQAQKIRRIIAQ
                     DFQEAFKQCDVIMGPVAPSVAWDLGAKGDDPVQMYLADIYTLSVSLAGLPGMSVPCGF
                     GAGANAQRPV"
     gene            complement(1412..1711)
                     /gene="gatC"
                     /locus_tag="VHV99_26390"
     CDS             complement(1412..1711)
                     /gene="gatC"
                     /locus_tag="VHV99_26390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012399478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEX3638948.1"
                     /translation="MALTLTDVKRIAHLARLELADAEAEHTLVQLNDFFGLVEQMQAV
                     DTTGIAPLAHPIEQIEDVALRLRDDAVTETVEREAFQRPAPAVQDGLYLVPKVIE"
     gene            2092..>3067
                     /locus_tag="VHV99_26395"
     CDS             2092..>3067
                     /locus_tag="VHV99_26395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX3638949.1"
                     /translation="MFGFLRSYFSNDLAIDLGTANTLIYMRGKGIVLDEPSVVSIRQE
                     GGPNGKKTIQAVGKEAKQMLGKVPGNIEAIRPMKDGVIADFTVTEQMIKQFIKTAHES
                     RMFSPSPRIIICVPCGSTQVERRAIKEAAHGAGASQVYLIEEPMAAAIGAGLPVSEAT
                     GSMVVDIGGGTTEVGVISLSGIVYKGSVRVGGDKFDEAIVNYIRRNYGMLIGETTAEL
                     IKKTIGSAFPGSEVKEMEVKGRNLAEGIPRSFTVSSNEILEAFTDPLNSIVSAVKSAL
                     ERTPPELGADIAERGMMLTGGGALLRDLDRLLAEETGLPLLVAEDPLTC"
BASE COUNT          580 a          977 c          941 g          569 t
ORIGIN      
        1 caccggacgc tgcgcattcg cgcccgcgcc gaagccgcac ggcacgctca tgcccggcaa
       61 accggcgagg ctcaccgaca gcgtgtagat gtcggccaga tacatctgca ccggatcgtc
      121 gcccttcgcg ccgaggtccc acgccaccga cggcgcgacc ggccccatga tcacgtcgca
      181 ctgcttgaac gcttcctgga agtcttgcgc gatgatccgg cggatcttct gcgcctgcag
      241 gtagtacgcg tcgtagtagc catgcgacag cacataggca cccaccagaa tgcggcgctt
      301 cacttccgga ccgaagcctt ccgcccgcga cttcttgtac atgtcgagca gatcgcggta
      361 ttccgcggcg cgatggccga agcgcacgcc gtcgaaacgc gacaggttcg acgaagcttc
      421 cgccggcgcg atcacgtagt acaccgggat cgacagttcg gttttcggca gcgaaacatc
      481 cactagcgtt gcgccgagtg cttcgtattg cttcaaggcg gcgtcgatcg aggcgcgcac
      541 gtcgtccgcg aggcctgcgc cgaagtattc cttcggcagg ccgatgcgca ggcccgcgag
      601 cggcttgtcc gcgctgtctt ctttccacgt cttgccgata tagcgcgtga agtcttcgtt
      661 ctcgcgcgtg aggctggtcg aatcgcgctc gtcgaaacca gccatggcgt tgagcagcgt
      721 cgcgcaatcc gcggcgcttt gcgccatcgg gccgccctga tccagcgacg aagcaaacgc
      781 gatcatcccg taacgcgaga cacggccata cgtcggcttg atgccggtga tgccggagaa
      841 cgatgccggc tgacggatcg agccaccggt atccgtgccg gttgcagcgg gcgcaagacg
      901 agcggccacg gccgctgccg agccacccga cgaaccgccc ggcacagcct tacgatccca
      961 cggattctgc acgggaccaa agtacgaatt ctcgttggac gagcccattg cgaactcgtc
     1021 catgttggtc ttgcccacgc acaccatgcc ggcgttctgc agacgcgcga ccaccgtcgc
     1081 gtcgaacgga cttgcgtagt tcgacagcat cttcgagccg gccgtggagc gccacccctt
     1141 ggtgacgaat acgtccttgt gcgcgatcgg caggccgacc agcgggccgg cgtggccggt
     1201 gtgaagcagc gcgtcggcag ctttggcctg cgcgagcgtc aggtcggcgt cgacctgaat
     1261 gaaggcgttc aggctgttgg ccgcgtcgat ccgcttcaga tacagctgcg ccagttcgac
     1321 tgcggagcat tccttggccg ccagtgcggc acgcagttcg gtcaagcttt tttcatgcat
     1381 tgcgttttat cctgggcttt attcgcggtt cttactcgat caccttcggc acgaggtaca
     1441 ggccgtcttg cacggccggc gccgggcgct ggaaggcttc gcgttcgacc gtttcggtca
     1501 ccgcgtcgtc gcgcagacgc agcgcgacgt cttcgatctg ctcgatcgga tgggcaagcg
     1561 gggcgatgcc ggtggtatcg accgcctgca tctgctcgac gaggccgaag aaatcattga
     1621 gctggacaag cgtgtgttca gcctcggcat cagccagttc gagccgcgcg agatgcgcga
     1681 tgcgtttaac atcggtcagg gtcagagcca tgcagtcacc ggaaaatgtg gtggaccgcc
     1741 gcagcgcgag aaaaacgatg ctgcgacagc gggttacgac actggaggaa tgcctcgaaa
     1801 aaggggccag cggtggcaaa aagtgccgtc cgtttccttc aaaacaacca aaattataag
     1861 gtatcattac gcgttcgacc caaacccgga ccgacttacc aaggcctttc tgaactattc
     1921 ccaaagatcg ttcggatttt tctgacctgg ccttgcaccg gcctccggta gactccctcg
     1981 cagcttcaag ttcctgtggc gccgcttccg cccggttgaa gctgttattt tttccgctgc
     2041 cgccctgcgc aaacgttgcg agggccggcc ccaagcgaga caggattttg aatgttcggt
     2101 tttctgcgca gctacttctc caacgatctg gcgattgacc tcggcaccgc caacacgctc
     2161 atctatatgc gtggcaaggg tattgttctt gacgaaccgt cagtggtctc gatccgccag
     2221 gaaggcggtc ccaacggtaa gaaaaccatt caggcagtcg gcaaggaagc caagcagatg
     2281 cttggcaagg tgccgggcaa catcgaggcg atccgcccga tgaaagacgg cgtgatcgcc
     2341 gacttcaccg tcaccgaaca gatgatcaag cagttcatca agacggctca cgaatcgcgt
     2401 atgttctcgc cgtcgccgcg catcatcatc tgcgtgccgt gtggttcgac ccaggtcgag
     2461 cgtcgtgcga tcaaggaagc ggctcacggc gcgggtgcgt cgcaggtcta cctcatcgaa
     2521 gaacccatgg cagcggcaat cggcgcaggc ctgccggtgt cggaagcaac cggctcgatg
     2581 gtcgtcgaca tcggcggcgg cacgaccgaa gtcggcgtga tctcgctcag cggcatcgtg
     2641 tacaagggct cggtgcgcgt cggcggcgac aagttcgacg aagccatcgt caactacatc
     2701 cgccgcaatt acggcatgct gatcggcgag accaccgccg agctcatcaa gaagacgatc
     2761 ggctccgcgt ttcccggctc ggaagtcaag gaaatggaag tcaagggccg caacctcgcc
     2821 gagggcatcc cgcgcagctt cacggtgtcg tcgaacgaaa ttctcgaagc gtttacggat
     2881 cctttgaaca gcatcgtctc ggcggtgaag agcgcgctcg agcgcacgcc gcccgagctg
     2941 ggcgccgaca tcgccgagcg cggcatgatg ctgacgggcg gcggcgcgct gttgcgcgac
     3001 ctcgatcgcc tgctcgcgga agaaaccggc ctgcccttgc tcgtcgccga agacccgctc
     3061 acgtgcg
//
LOCUS       DATASH010000990         5446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3336305,
            whole genome shotgun sequence.
ACCESSION   DATASH010000990 DATASH010000000
VERSION     DATASH010000990.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5446
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3336305"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VHV99_26400"
     CDS             complement(<1..195)
                     /locus_tag="VHV99_26400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638950.1"
                     /translation="MCDASILTPDAKIGAVPGLAPTPYSFQDDCRGLRIPSIVKANHS
                     MPTVAIAIFPGVQALDVAGPV"
     gene            213..1154
                     /locus_tag="VHV99_26405"
     CDS             213..1154
                     /locus_tag="VHV99_26405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3638951.1"
                     /translation="MVIKNLLRRLDLTTLQLFLAVYEEGTLTRAAEREAIAVSAASKR
                     LLELEQAVGATLFQRNARGMTLTPAGETLLHHARRVMRDIENIGIELAGHASGVRGYV
                     RMMANLSAIVEFLPEDLRAFQLQYERVKLDLEERPSGGVVEAVDDSLADLGICSGDAD
                     TRDLHVAHYRHDSLVLVMRDDHPLAARASIAFAETLDGDHVGLHAASSINARTHMAAR
                     QTGKPLRLRIHVPGFDAVCRMVQAGMGVGVLPLKVYQLMGRSLGLVGVPLEDEWSERS
                     LVLVVRDVEALSPVSRLLFDHLRSIEASEAPLAVAGT"
     gene            1260..2453
                     /locus_tag="VHV99_26410"
     CDS             1260..2453
                     /locus_tag="VHV99_26410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX3638952.1"
                     /translation="MGGPLQGIRVIEIGTLIAAPFAARLMAEFGAEVIKIEAPGTGDP
                     LRKWRKLHEGTSLWWYLQSRNKKSICVNLKSADGIDIVKRLAADADVVIENLRPGALE
                     KLGLGWDVLHAINPKLTMVRISGYGQSGPYRDRPGFGAIGEAMGGIRYTTGEVDGAPA
                     RVGVSLGDSLASLHGVIGALMSLLRVKTGQGEGQVVDVSLVESVFNLMESLVPEYDLL
                     GHVRERSGGALPGIAPSNTYPTEDDGYVVIAGNSDPIFKRLMQVIGRTDLADDPALAH
                     NDGRAKQCALLDQAIAAWTSHHSTNDVLAALERAEVPAGRIYSVAEIVADPHYQARDM
                     LLQTQLPGGASVKMPGIVPKLSDTPGEVHWQGPTLGEHTGSVLSALGFANEDIERLRR
                     EGAVQ"
     gene            2450..3397
                     /locus_tag="VHV99_26415"
     CDS             2450..3397
                     /locus_tag="VHV99_26415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013342157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEX3638953.1"
                     /translation="MKASDTNLDKLIVQEVAPRDGLQIEPTWVDTADKIALINALSTA
                     GFTRIEAGSFVSPKAIPALRDGEAVFQQIERHPGVIYVALIPNLKGAERALASRADEL
                     NLVMSASQTHNRANMRMSCEASLAAFGDIVRHVKASGVLLNASVATAFGCPFEGKIDE
                     DRVIGIVDTYREMGIQGITLADTTGMANPRQVSRLVTRVLERLPVSALTLHFHNTRGL
                     GLANVLAAYEAGARRFDAALGGLGGCPFAPGASGNICTEDLVNMCDEIGIPTAIDLEK
                     LLALSRGLPALLGHDVPGQLAKAGRNRDRHPVPEYVLQI"
     gene            3459..4790
                     /locus_tag="VHV99_26420"
     CDS             3459..4790
                     /locus_tag="VHV99_26420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3638954.1"
                     /translation="MSDLGAVASGGREYSADDAVAIIRKVAWRLMPLIMVCYLFAFFD
                     RINISFAKFQLQSDLGLSDTAYGLGASLFVIGYVLFEVPSNLFLYKVGARRWIARIMI
                     SWGLATAAMVFVNNEWQFYGLRFVIGAMEAGFAPGVLYYLTLWFPPSYRGRITSMLFL
                     ASAFSGLVGAPLAGLVIGHMNGVLGMPGWHWLFLLGGLPCVLLGYLVLKLLKDRIEDA
                     GWLSSTEKSFLATQIAQQSRHPGTGHSLLGAIRTPGFLTLGLVYFLIQVASYGLNFWA
                     PHLIRVAGTNNPTVIGLLTAVPYVCGAICMVVVGRLSDASGERRKFVFGLLVMAAVGF
                     FAAGFFDKQTTLLVVALAVMGAGVVASIPAFWALPPKLVTGAGAAGGIALINTLGQLG
                     GIVSPVMVGRVKDLTGSTTPALYVIGAMSLVCALIVLYGLPDSLRQKDAAL"
BASE COUNT          945 a         1667 c         1756 g         1078 t
ORIGIN      
        1 gacgggaccg gcgacatcga gcgcctgcac gccgggaaag atcgcgatcg cgacggtagg
       61 catcgaatga tttgctttga caatggacgg aatccgcaga ccgcggcagt cgtcctggaa
      121 ggaataggga gtaggggcta agccgggcac cgcacctatt ttcgcatcag gcgtcaaaat
      181 agacgcatcg cacaaccacg cggcgctccg ccatggtaat caaaaacctt ctacgaaggc
      241 tggacctcac cacgctccaa ctcttcctcg ccgtctacga agaaggcacg ctcacgcgtg
      301 ccgcggagcg cgaggccatt gccgtatccg ccgcgagcaa acgtctgctc gaactcgaac
      361 aggcagtcgg cgccacgctt tttcagcgca acgcgcgcgg catgacgctg acacccgccg
      421 gcgaaacgct gctgcatcac gcgcgccggg tgatgcgcga catcgaaaac atcggtatcg
      481 aactggccgg ccatgcgagc ggcgtgcgcg gctacgtgcg gatgatggcc aacctctcgg
      541 cgattgtcga gtttctgccg gaggatttgc gcgccttcca gttgcagtac gaacgtgtca
      601 agctcgatct cgaagagcgg ccgagcggcg gcgtggtgga agccgtcgac gacagcctcg
      661 ccgacctcgg catctgttcc ggcgacgccg acacgcgcga tctgcacgtt gcgcactatc
      721 ggcacgattc gctggtactg gtgatgcgcg acgatcatcc gctggccgcg cgcgccagca
      781 tcgccttcgc cgaaacgctg gacggcgatc acgtcggcct gcatgcggcc agttcgatca
      841 acgcccgcac ccacatggcc gcacgccaaa ccggcaagcc gttgcgtttg cgcatccacg
      901 tgccgggttt cgacgcggtc tgccgaatgg tgcaggccgg catgggcgtc ggcgtcttgc
      961 cgctgaaggt gtatcagctc atgggccgct cgcttggcct cgtcggcgtg ccgctggaag
     1021 acgaatggtc cgaacgcagc ctcgtgctgg tggtgcgcga cgtcgaggca ctgtcgccag
     1081 tgagccgttt gttgttcgac cacttgcgtt cgatcgaggc gtccgaagcc ccgctggccg
     1141 tcgccggcac ctgagcgttc gccgttcgcg aacggttgtt ggcgaattgc ggttggacgc
     1201 cgttgcagtg ctgctcctaa gctgtctctc aacacgaaac cagcttggga gacagcatta
     1261 tgggtggacc ccttcaaggt atccgcgtca tcgagatcgg aaccctcatc gccgcgccgt
     1321 ttgccgcgcg cctcatggcc gagttcggcg ccgaagtcat caagatcgaa gcccccggca
     1381 ccggcgaccc ccttcgcaaa tggcgcaagc tgcacgaggg cacctcgctg tggtggtatc
     1441 tgcagtcgcg taacaagaaa tcgatctgcg tgaacctgaa gtcggccgac ggtatcgaca
     1501 tcgtcaaacg gctcgccgcc gatgccgacg tcgtgatcga aaatctgcgc cccggcgcgc
     1561 tcgaaaagct cggcctcggc tgggacgtgc tgcacgcgat caatcccaag ctgacgatgg
     1621 tccgcatctc cggctacggc cagtccggcc cgtaccgcga ccggccaggc ttcggcgcga
     1681 tcggtgaagc gatgggcggc atccgctata cgacaggcga agtggacggc gcaccggcac
     1741 gcgtcggtgt cagcctcggt gattcactcg cttcgctgca tggcgtgatc ggtgcgttga
     1801 tgtcgctgct gcgcgtcaag acgggacaag gcgaaggtca ggtggtggac gtctcgctgg
     1861 tcgaaagcgt gttcaatctg atggaaagcc tcgtgcccga atacgacctg ctcggtcacg
     1921 tgcgcgagcg cagcggcggc gcgttgcccg gcatcgcgcc gtcgaatacc tatccgaccg
     1981 aagacgacgg ctatgtcgtg atcgccggca acagcgatcc gatcttcaag cggctgatgc
     2041 aggtaatcgg ccgcaccgat ctcgccgacg atcctgcgtt ggcccacaac gacggccgcg
     2101 cgaagcaatg cgcgctgctc gatcaggcga tcgccgcatg gacttcgcac cattccacca
     2161 acgatgtact cgccgcgctc gaacgcgctg aagtgccggc gggacgcatc tattccgtgg
     2221 ccgagatcgt cgccgatccg cactaccagg cgcgcgacat gttgttgcaa acgcaactgc
     2281 ctggcggcgc ctcggtgaag atgccgggca ttgtgccgaa gctgtcggac acgccgggcg
     2341 aagtgcactg gcaaggtccc acactgggcg agcacaccgg cagcgtgctg agcgcactcg
     2401 gattcgccaa cgaagatatc gagcggctgc gccgcgaggg agctgtgcaa tgaaagcgag
     2461 cgacacgaat ctcgacaagc tgatcgtgca ggaagtcgcg ccgcgcgacg gcttgcagat
     2521 cgaacccaca tgggtcgaca ccgccgacaa gatcgcgctg atcaacgcgc tttcgactgc
     2581 gggcttcaca cgcatcgagg cgggctcgtt cgtctcgccg aaagcgattc cggcgttgcg
     2641 cgacggcgaa gccgtgttcc agcagatcga gcgtcatccg ggcgtcatct acgttgcgct
     2701 gattccgaat ctgaagggcg ccgaacgcgc gctggcctcg cgtgccgatg aattgaatct
     2761 ggtgatgtcg gcgagccaga cgcacaaccg cgccaatatg cggatgagtt gcgaagcgtc
     2821 gctggcggcg ttcggcgata tcgtgcgcca cgtcaaggcg tcgggcgtgt tgctgaatgc
     2881 ctcggtggcg accgcgttcg gttgtccgtt cgaaggcaaa atcgacgaag accgcgtgat
     2941 cggcattgtc gatacgtatc gcgagatggg catacagggc atcacgttag ccgatacgac
     3001 cggcatggcg aatccgcgcc aggtttcgcg gctagtcacg cgcgtgcttg aacgactgcc
     3061 ggtttccgcg ctcacgctgc acttccacaa cacgcgcggt ctgggtctcg ccaacgtgct
     3121 ggccgcttac gaagccggcg cgcgccgctt cgacgcagcg ttgggtggcc tcggcggctg
     3181 cccgttcgca ccgggtgcct ccggcaacat ctgcaccgaa gacctcgtga atatgtgtga
     3241 cgaaatcggc atcccgactg cgatcgacct ggaaaagttg ctcgcgttgt cgcgcggatt
     3301 gcctgcgctg ctcggccatg acgtgcccgg gcaactggcg aaggccggcc gtaaccgcga
     3361 ccggcacccc gtgcccgaat acgttctaca gatctgacac tttccctaac tgattacacg
     3421 accggacggc atcgccgaag ccggcattgg agacaagcat gagtgacctt ggcgcagtcg
     3481 caagcggcgg ccgtgagtac agcgcggacg acgcagtggc aatcatccgc aaagtagcgt
     3541 ggcggctcat gccgctcatc atggtctgct acctgttcgc gtttttcgat cgcatcaata
     3601 tcagcttcgc gaagttccag ttgcaaagcg accttgggct ctccgatacc gcgtatggtc
     3661 tcggcgcgag tctcttcgtg atcggctatg tgctgttcga ggtaccgagc aatctgtttc
     3721 tgtacaaggt tggcgcgcgt cgctggattg cgcggatcat gatttcatgg ggcctcgcga
     3781 cggcggcgat ggtgttcgtc aacaacgaat ggcagttcta tggactgcgc tttgtgatcg
     3841 gcgcaatgga agcgggcttt gcgccgggcg tgctgtatta cctcacactg tggtttccgc
     3901 cgagctatcg cggacgcatt acgtcgatgc tgtttctggc gtccgcgttc tccggtttgg
     3961 tcggcgcacc gctcgcgggg ttggtgatcg gccatatgaa tggcgtgctc ggcatgccgg
     4021 gctggcactg gttgttcctg cttggcgggc tgccgtgcgt tttactcggc tatctcgtgt
     4081 tgaagctgct gaaggatcgg atcgaggatg cggggtggtt gtcgtcgacg gagaagtcct
     4141 tcctggcgac gcagattgcg cagcaaagcc ggcatcctgg cacggggcat tcgttgctcg
     4201 gcgcaatcag gacgccagga ttcctgacct tgggcctggt gtacttcctg attcaggtgg
     4261 cgtcgtacgg cttgaatttc tgggctccgc atttgattcg cgtcgcgggc acgaacaatc
     4321 cgacggtgat tggtttgctg acggcggtgc cttatgtgtg cggtgcgatc tgcatggtcg
     4381 tggtggggcg gctgtctgac gcgagcggcg agcggcgcaa gtttgttttc ggattgctgg
     4441 tgatggcggc ggttggattc tttgccgccg ggttcttcga caagcagact acgttgctgg
     4501 ttgtcgcatt ggcggtgatg ggcgctgggg tggttgcttc gattccggcg ttctgggcac
     4561 tgccgccgaa gttggtcacg ggcgcgggcg cagccggcgg gattgctttg atcaacacgc
     4621 tcggccagct tggcgggatt gtcagccctg tgatggtcgg ccgggtgaag gatctgaccg
     4681 gtagtacgac gccggcgttg tatgtgatcg gcgcgatgag tctggtttgc gcgttgatcg
     4741 tgttgtatgg gttgcctgat tcgctacggc aaaaggatgc cgcactttga tggcggcagt
     4801 tgcgtggacg aaagcgccgc ggccgcgcga catgacggtc agcctctgcc atgcggcgct
     4861 gcgatctcct gcaaatccag atcgtgttcg aggctggtca gcagtgcatc atgaatcaga
     4921 cccggtcggt tggttttgtc tgcgtggcgc ttgtcgtttt tatgcgtgcg gtgttgggcc
     4981 tttccttgaa ctggctcttt ggcctttcct tgatttgtta tcggtctatt agcgtcgccc
     5041 ctgtgcgggg cgggcaccta cttttctttg cctgccgcaa agaaaagtag gcaaaagaaa
     5101 gcggctcaca ccgccagcgc ataagcgggt cccccgcgca gtcgcggtag tggtgcatct
     5161 ggaatccgtg ccatcgcaca ttcggcgtga gtgacaaggg cgtcatcagc tcccactccg
     5221 cactgcgtgc gtcgcggatg ggtatgcatg ggaaaccagt ggcttcgctt cgcgcactgg
     5281 gagccatcgg cttcgcctcg gcgatgcgcc tccgcactca tcagcaaaca cccgcttcaa
     5341 gtcaatgtcg agtgtcgcac cagcacccgc acccgcaccc gcacccgcac ccgcacccgc
     5401 acccgcaccc gcacccgcac ccgcacccgc acccgcaccc gcaccc
//
LOCUS       DATASH010000991         7178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5710972,
            whole genome shotgun sequence.
ACCESSION   DATASH010000991 DATASH010000000
VERSION     DATASH010000991.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7178
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5710972"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(362..1186)
                     /gene="hpnK"
                     /locus_tag="VHV99_26425"
     CDS             complement(362..1186)
                     /gene="hpnK"
                     /locus_tag="VHV99_26425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis-associated protein HpnK"
                     /protein_id="HEX3638955.1"
                     /translation="MAAKLRGLIVTADDFGLDHRVNLAVEQAHRHGVLTAASLMIGAP
                     AAGDAVARARALPALRVGLHLVLADGDAVAPRAGIAALLDEHGRFGDNMVRDGVRFFF
                     LPHVRKQLAREIRAQFEAFARTGLTLDHVNTHKHFHVHPTVLGLILEIGREYGMRAMR
                     LPFEASAPLWLRPWISQVRGRLDRAGIVHNDYVVGIANSGRMDEAAWLAALADLPAGV
                     GEIYCHPAVAGDRVLSRGMREYRHVDELQALLSARVADVIRESGARVGGFGDVLKG"
     gene            complement(1176..2468)
                     /gene="hpnI"
                     /locus_tag="VHV99_26430"
     CDS             complement(1176..2468)
                     /gene="hpnI"
                     /locus_tag="VHV99_26430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohopanetetrol glucosamine biosynthesis
                     glycosyltransferase HpnI"
                     /protein_id="HEX3638956.1"
                     /translation="MLARALTACQWILLSGCVPASLYAMLAAVAMPFFAARRSGAASV
                     AHAASAAARTAHLQHPFAHIGVSVLKPLCGAEPRLYENLRTFCDQRHGHFQLVLGVSS
                     PDDAAIAVVRRLQAAFPMRDIELAVDTRVHGSNLKVSNLINMAERARHDVIVIADSDI
                     AVEVDYLDSVAAPLADPRVGVVTCLYVAQSVGGFWPRVGALFINEWFAPSVRVAHAAG
                     SQRFGFGATLALRRATLERIGGFEALKNCLADDYWLAEHVRALGLRTVLSRVMVATDV
                     IEPTFSALWQRETRWLRTIRSVSPRGFAFLFVTFPTPWLLAGAWLTSRLANGPIDGVH
                     LWAALASGVSTVAGFAARMLLHARAARHERTFWRDLPLVPLRDTLLACQWFAGAFGSH
                     VTWRGARVPVGAPSSAPTPTTHGAALDAMDVMETSDGG"
     gene            complement(2481..2822)
                     /locus_tag="VHV99_26435"
     CDS             complement(2481..2822)
                     /locus_tag="VHV99_26435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638957.1"
                     /translation="MKPVTADMLRAHLMAAALTAMGTEVLALLGLLVLPVSQQIFSST
                     TFQFIALALIVCGVLVMRQVARAAFDLAIQSHHATSAGQPVLIEADCPRRWFVILHLR
                     FTGRPFVLAGH"
     gene            3174..3833
                     /locus_tag="VHV99_26440"
     CDS             3174..3833
                     /locus_tag="VHV99_26440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007583686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3638958.1"
                     /translation="MRVLLVEDDDLIGCGIEAGLRQAGFTVDWARDGHKAGLALDTTA
                     YALVLLDLGLPKVPGMELLKRLRDAGKDVPVLVLTARGTVVDRVGGLEAGADDYLGKP
                     FDLTELIARCRALLRRAQGRSVEVIRYQDLTVNPAAQTAELGNTRVPLTSREWAILMQ
                     LLTNQGIPQSRSRLEESLYGWQEEIESNAIEVHVSNLRKKLGAKLIKTVRNIGYVVEK
                     T"
     gene            3833..5233
                     /locus_tag="VHV99_26445"
     CDS             3833..5233
                     /locus_tag="VHV99_26445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018417792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3638959.1"
                     /translation="MSASIRRRLVLLVLTSIVLIWGIALISSYRQASREVGEWEGARL
                     AELAQILALLDERNLTVLANARIDVREEEKGGEPGANNSDDDDTLPRDALFQVLSANG
                     EVLAGSPQLHTLRAWDLPVPPASGAQDMTLGGQMWHSFTLRDAALGHTVRVFEPANTR
                     SDLVSGVASRIARPTLVALPVLALLVWFSIGWSLAPLKTLSRAIRARDINRLEPVDIG
                     RVPTEVRPLVDAINLVLSRLRHSLEHERAFTADAAHELKSPLAAIKVQAQVALAEPDT
                     ALQRLAMERVVQGVDRSARLAEQLLLLARLDVHEKLSTARLRPAAVAKDALLANERNA
                     QQKNIRVTLIGDMRAEINAEPVLIGILLDNLLDNAIKYGRAGGCIEVAIQHEVGRVQL
                     CVRDDGPGVAPSDLARLTNRFFRATGTQATGSGLGLSIVARIAEHFGASLHLGTGIEE
                     HGLAVEVSFPAYGGAH"
     gene            complement(5255..6349)
                     /locus_tag="VHV99_26450"
     CDS             complement(5255..6349)
                     /locus_tag="VHV99_26450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEX3638960.1"
                     /translation="MKHLGRIAALAGLLVSLWLVWQENPVAVLGALRAAGAGLFLAAI
                     AHVLPMLANACDWRLLIRGANRPTIAAMLHLVWVRESVNGMLPVARVGGEVVSFRMLK
                     RWGVRPSMSVGSIVVDMQLTVISQLLFTMVGIGFLFADAHSDTLRLAGQLAWGVVVLT
                     PLLVLFALVQHASPFERITRVLNRMTSGKLAALVGQSAQIDQVIKLIWRRRGAVLRYL
                     FFWQPLQCFLTSLEIWLALYFLGVQITLVEAVVFESLIQAISSAAFFVPGALGVQEGG
                     FILIGGALGLDPSICLALAGARRIRDLLIFVPGLIAWQFAESSNREPRRMERTEPAER
                     TEPAEWAERTEPAELTEWAERPAVGETSRR"
     gene            complement(6439..>7178)
                     /locus_tag="VHV99_26455"
     CDS             complement(6439..>7178)
                     /locus_tag="VHV99_26455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018434626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3638961.1"
                     /translation="DYLRNVIGELQKPGVGLVTCVYRGAPDPGFWPRMSAKATNYQFL
                     PGVVTGLALGLARPCFGQTIAMRRETLEKIGGFTPFVKHLAEDHAIGEAVRMIGEKVV
                     IPPFTISHACVESSATQLIAHELRWSRTIRRIDRLGHLGSALIHPLAFALLAIAFSGG
                     ALWSWPLALVAVCVRLVLKLLSDRALQQAHRDLWLLPLWDIVSFSIFVASFWSSRVSW
                     RGFSFKVDGDGLLSAVQDGMKDEQQVG"
BASE COUNT         1566 a         2432 c         2105 g         1075 t
ORIGIN      
        1 tgctgtaaca ccagatccac cgcgtcttcc ttgaactgcg ccgagaattc ccgtcttccc
       61 atttgacacc tcatcgtttg gttagaagtc taccttttga ggtgtccagc tttattaggc
      121 cactacagtg cccgccccgc acaggggcga cgctaataga ccaagatcaa ttcaaggaaa
      181 ggccaaaaag ccagttcaaa caaaggccaa aaagccactt caagaaaagc ccaaaaaccc
      241 agttcaagaa aagaccaaca ccaaaaaacc acacccaaac aatcgcccca ggcaaaaaaa
      301 tcagcaaaac cccaacaccg caagcaaaca aagcaccaat cgccgcccag gcaaaaaaac
      361 ctcaaccctt caacacatcc ccaaacccgc ccacccgggc acccgactcc cgaatcacat
      421 cggcgaccct cgccgacaag agcgcctgca actcatcaac atgccgatac tcccgcatcc
      481 cacgactcaa aacccgatcg ccggcaaccg caggatgaca ataaatctcc cccaccccag
      541 caggtaaatc ggccagcgcg gcaagccacg ccgcctcatc catccgccca ctattagcaa
      601 tcccaacaac ataatcgttg tgcacgatcc cagcccgatc caacctcccc cgcacctgcg
      661 agatccacgg ccgcaaccac agcggcgcgc tcgcctcgaa cggcaaacgc atcgcccgca
      721 tcccatactc gcggccgatc tccagaatca acccaagcac cgtcggatgc acatgaaaat
      781 gcttgtgggt attgacatga tcgagcgtca aacccgtcct cgcaaaagcc tcgaactgcg
      841 cgcgaatctc ccgcgccaac tgtttgcgaa catgcggcaa aaagaaaaac cgcaccccgt
      901 cccgcaccat gttgtcgccg aaacggccat gctcgtcgag caatgcggca atccccgcgc
      961 gcggcgcgac cgcgtcgccg tcagccagca cgagatgcag gccgacccgc aacgcgggca
     1021 gcgcgcgagc acgcgccacc gcatcgcccg cggcaggtgc accgatcatc aggctcgcgg
     1081 ccgtcagcac gccgtgccgg tgcgcctgtt cgaccgccag gttcacccga tgatccagac
     1141 cgaaatcgtc ggcagtaacg atcagcccgc gcaacttagc cgccatccga agtctccatg
     1201 acatccatcg catccaacgc cgcaccatgc gtcgtcggcg tcggtgccga cgacggcgct
     1261 ccgaccggca cgcgcgcgcc acgccacgtc acatgcgaac cgaacgcgcc cgcgaaccat
     1321 tggcatgcca gcaaggtgtc gcgcaatggc acgagcggca aatcgcgcca gaaagtgcgt
     1381 tcatgacgcg cggcacgcgc atgcagcagc atgcgcgcgg caaaaccagc caccgtgctg
     1441 acgccgctcg cgagcgccgc ccacaggtgc acgccgtcga tgggaccgtt cgcaaggcgg
     1501 ctggtgagcc atgcgccggc aagcagccac ggcgtcggga aggtgacgaa caaaaatgca
     1561 aagccacgtg ggctcaccga gcggatcgtg cgcaaccagc gcgtctcacg ctgccacaac
     1621 gcggaaaaag tcggctcgat gacatcggta gccaccatca cgcgcgaaag caccgtccga
     1681 agcccgagcg cgcgcacgtg ttcggcgagc cagtaatcgt ccgcgagaca gtttttcagc
     1741 gcctcgaatc cgccgatccg ctcgagcgtc gcgcgccgta acgcaagcgt cgcgccgaaa
     1801 ccgaaccgtt gcgaccctgc cgcatgcgcg acccgcaccg atggcgcaaa ccactcgttg
     1861 atgaatagcg cgccaacccg tggccagaaa ccgccgacgc tttgcgccac gtagagacag
     1921 gtcaccaccc ccacacgcgg atcggcgagc ggcgccgcca cgctgtcgag ataatcgact
     1981 tcgaccgcga tgtcgctatc cgcaatcaca atcacgtcgt ggcgcgcccg ctccgccatg
     2041 ttgatcagat tgctgacctt gagattgctg ccgtgtaccc gcgtatcgac cgctagctcg
     2101 atgtcgcgca tcggaaacgc ggcctgcaaa cggcgcacca cggcgatggc ggcatcgtcc
     2161 ggcgacgaga caccgagcac gagctggaag tgcccgtggc gctggtcgca gaaagtcctg
     2221 agattctcgt agagacgtgg ctcggcgccg catagcggct tgagcacgct gacgccgatg
     2281 tgcgcaaaag gatgctgaag atgcgcagtc cgggcggcgg cactcgcggc gtgcgcaaca
     2341 ctcgcggccc cgcttcggcg cgcggcgaag aacggcatgg ccaccgcagc cagcatcgca
     2401 tagagcgagg ccggaacgca gccggataac agaatccact ggcatgccgt caatgcgcgg
     2461 gccaacatcg cctgtttccg tcagtgcccg gccagcacga atggtctgcc cgtaaagcgc
     2521 agatgcagaa tcacgaacca gcgccgcggg caatccgctt cgatcaaaac gggctggccg
     2581 gcagacgttg cgtggtggct ttgaatcgcg aggtcgaacg ctgcgcgcgc gacttggcgc
     2641 atcacgagga cgccgcagac gatcagtgca agcgcgatga actggaacgt cgtggaacta
     2701 aagatctgct gggaaaccgg caagaccagc agccccagca aggcgagtac ttcggtaccc
     2761 atggccgtga gcgcagccgc catcagatgg gctcgcagca tgtcggcggt gactggcttc
     2821 atggcttggt ctcgtgacct gaacaaacgc acgccaacgt ggcgtggcgc gaatgactgg
     2881 caaaagcgcc gacgcaaggc accgggaaaa gcgtcggccg tacatcccgt cgaagtgctt
     2941 acgattaagt tgcgatgatc tgacaacacg acgtcgtttc gcatgctcat atcagccacg
     3001 cgatagtgac ctgttgagcc gcgatagtag cgattcgaac tgaaggaaac cttaaggaag
     3061 aaagaggtat aaagcgtgtc atgaacatgc tcgttacact gcgggtatgc ggctcggcgc
     3121 ttactcaaaa gccactgccg ctatggaatg gacatcgaac ggaatttctc cccatgcgtg
     3181 tactcctcgt agaagacgac gacctgatcg gctgcggcat cgaagctgga ctgcgtcaag
     3241 ccggtttcac ggtcgactgg gcacgcgacg gccacaaggc cggcctcgcc ctcgacacca
     3301 ccgcctatgc actggtacta ctcgatctcg gcttacccaa agtgccaggt atggaactgc
     3361 tgaaacggct gcgcgacgcc ggcaaggacg tgccggtgct ggtgctgacc gcgagaggca
     3421 cggtggtgga ccgcgtcggc ggcctcgaag ccggcgccga cgactacctt ggcaagccct
     3481 tcgatctgac cgagctgatc gcccgctgcc gggcgctgct gcgccgcgct caagggcgca
     3541 gcgtcgaggt gatccgctat caggatctga cggtcaatcc cgccgcgcag accgccgaac
     3601 tcggcaatac gcgcgtgccc ctcacttcgc gtgaatgggc gatcctgatg cagttgctga
     3661 ccaaccaggg cattcctcag tcgcgctcgc gccttgaaga gagtttgtat ggatggcagg
     3721 aagaaatcga aagcaatgcg atcgaggtcc atgtgtccaa tctgcgcaaa aaactcggcg
     3781 ccaaattgat caagaccgtg cgcaatatcg gctacgtggt ggagaaaacg taatgtcggc
     3841 atccatccgc cggcgcctgg tgctgctggt cctgaccagt atcgtgctga tctggggcat
     3901 cgcgctgata tcgagctatc gtcaggcctc gcgcgaagtc ggtgaatggg aaggggcgcg
     3961 ccttgcggag ctcgcgcaaa tcctcgcgct gctcgacgaa cgcaatctga ccgtgctcgc
     4021 caacgcgcgc atcgacgtgc gcgaagaaga aaaaggcggc gaacccggcg ccaacaacag
     4081 cgacgacgac gacaccttgc cgcgtgacgc gctcttccag gtactcagcg cgaatggcga
     4141 ggtactcgcc ggcagcccgc aattgcacac gctgcgcgcg tgggatttgc ccgtgccgcc
     4201 cgcaagcggc gcgcaagaca tgacgctggg cggtcagatg tggcactcgt tcaccttgcg
     4261 cgatgctgcg ctcggccaca ccgtgcgtgt gttcgaaccg gccaatacac gcagcgacct
     4321 ggtgagcggg gtcgcgagcc ggatcgcccg gcccacgctc gtcgcgctgc cggtgctggc
     4381 gctgctggtg tggttcagca tcggctggag cctcgccccg ttgaagacgc tctcgcgcgc
     4441 catccgcgcg cgtgacatca accgtctcga accggtcgac atcggccggg tgccgaccga
     4501 ggtacggcca ctcgtcgacg cgatcaatct ggtgctgtcg cgcttgcggc actccctcga
     4561 gcacgaacgc gcgtttaccg ccgacgccgc ccatgaactg aagtcgccgc ttgccgccat
     4621 caaggtgcag gcacaggtcg cactggccga accggacacg gcgctacagc ggctggcgat
     4681 ggaacgcgtg gtgcaaggtg tcgatcgcag cgcgcggctc gccgaacagt tgctgctgct
     4741 cgcgcgtctg gatgtgcacg aaaaactctc gaccgcgcga ctcaggcccg cggcggtggc
     4801 gaaagacgcg ttgctcgcca acgaacgcaa tgcgcagcaa aagaacattc gcgtcacgct
     4861 catcggcgac atgcgagccg agatcaatgc ggagcccgtg ctaattggca tcctgctcga
     4921 caatctgcta gataacgcca tcaaatacgg acgcgcgggc ggctgtatcg aggtggcgat
     4981 acagcatgag gtcggccggg tgcagctctg cgtgcgcgac gacggccccg gcgtcgcgcc
     5041 cagcgatctc gcgcgcctca cgaaccggtt cttccgcgcg accggcactc aggccaccgg
     5101 cagcgggctg ggcctctcga tcgtcgcgcg catcgccgag catttcggtg cgagcctgca
     5161 tctcggcacg gggatcgaag agcacggcct cgcggtggag gtgtcgtttc ccgcttatgg
     5221 gggcgcgcat tgagggtgtc gcgctcgcgc cgcgctagcg ccggctcgtt tcacccacgg
     5281 ccggccgctc tgcccattcc gtcagttctg ccggttccgt tcgctctgcc cattctgccg
     5341 gttccgttcg ctccgccggt tccgtccgct ccatccgcct cggctcgcga ttcgacgact
     5401 cggcaaactg ccacgcaata agtcctggca cgaagatcag cagatcacga atgcgccgcg
     5461 cgccggcgag cgcgaggcag atggaaggat cgagacccag cgcgccgccg atcagaatga
     5521 agccgccctc ctgcacaccc aaggcgcccg gtacgaagaa cgctgcactg ctgatcgcct
     5581 ggatcagcga ttcgaacacc accgcttcga ccagcgtgat ctgaacgccg agaaagtaca
     5641 gcgcgagcca gatctccagt gacgtcagga agcactgcag cggttgccag aagaacagat
     5701 agcgcagcac cgcgccgcgc cggcgccaga tcagcttgat gacctggtcg atctgcgcgg
     5761 actgcccgac gagcgcggcg agcttgccgc tcgtcatacg attgagaaca cgcgtgatgc
     5821 gctcgaacgg actggcatgc tgcacgagtg cgaacaacac cagcagcggg gtcagcacca
     5881 ccacgcccca tgccagttga ccggcgagcc gcagagtatc ggagtgcgca tccgcgaaca
     5941 gaaaaccgat gccgaccatc gtgaacagca actggctaat gaccgtgagc tgcatatcga
     6001 cgacgatgct gccgaccgac atggacggcc gcacgcccca gcgcttgagc atccggaacg
     6061 acaccacctc gccgccgaca cgcgccaccg gcaacatgcc attgacggat tcgcgcaccc
     6121 agaccagatg cagcatcgca gcgatagttg gccggttcgc gccgcgaatc aacaagcgcc
     6181 aatcgcaggc attggcgagc atcggcagaa cgtgcgcgat cgcggccaga aaaagcccgg
     6241 cgcccgcagc acgcaatgcg ccgagtacgg cgacgggatt ttcctgccag accagccaca
     6301 acgacaccag caagccggca agcgcggcga tgcggccgag atgcttcatc acgcggccac
     6361 cctgcgcgcg tgcttcaagg ccgtcgtgcc cgctgacctg ccgtcactag ccgcttcaaa
     6421 agggccaccc atcatgcctt acccaacctg ctgttcatcc ttcattccat cctgcacggc
     6481 cgacaacagg ccgtcgccat ccaccttgaa actgaaaccg cgccagctca cgcgcgagga
     6541 ccagaagctc gccacgaaaa tcgaaaacga tacgatatcc cacaacggca agagccacag
     6601 gtcgcgatgc gcctgctgca acgcgcgatc ggacagcagt ttcaggacga gccgcacgca
     6661 tacggcaacc agcgcgagcg gccacgacca cagcgcgccg cccgagaagg cgatggcgag
     6721 cagcgcgaaa gcgagcggat gaatcaacgc cgagccgaga tggccgagcc gatcgatgcg
     6781 gcgaatcgta cggctccagc gcagttcgtg cgcaatcagt tgcgtcgcgc tcgattccac
     6841 gcaggcatgc gagatcgtga acggtggaat caccaccttc tcgccgatca tgcgcacggc
     6901 ctcgccgatt gcatggtctt cggccagatg cttcacgaac ggcgtgaatc caccgatttt
     6961 ttcgagggtt tcgcgtcgca tcgcaatggt ctgcccaaag cagggtcggg caagaccgag
     7021 cgccaggcct gtcaccacgc cgggcaggaa ctggtagttg gtggccttgg ccgacatgcg
     7081 cggccagaaa ccaggatcgg gcgcaccacg atacacgcag gtgacgagac cgacgccagg
     7141 cttctgcaac tcgccgatca cattgcgcag ataatccg
//
LOCUS       DATASH010000992         3544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2741391,
            whole genome shotgun sequence.
ACCESSION   DATASH010000992 DATASH010000000
VERSION     DATASH010000992.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2741391"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..164
                     /locus_tag="VHV99_26460"
     CDS             <1..164
                     /locus_tag="VHV99_26460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3638962.1"
                     /translation="RTITLPVRMYMYATESIDPMMASVSALVIFITAGAMIVLDRVYG
                     LNRILIGSH"
     gene            197..550
                     /locus_tag="VHV99_26465"
     CDS             197..550
                     /locus_tag="VHV99_26465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX3638963.1"
                     /translation="MTNLPSATSTGQPHTATDAAPQFVRLTEAHRKQVTLTLDGVAIT
                     ALTGDTLLSAILLHTRRVRDTEFSGEPRAGFCLMGACQDCWVQLEEGTRIRACSTFVE
                     EGLRVMTRPAGATGT"
     gene            574..2004
                     /locus_tag="VHV99_26470"
     CDS             574..2004
                     /locus_tag="VHV99_26470"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012405813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HEX3638964.1"
                     /translation="MHRVVIAGAGPAGVRAAQTLVAAGIVPVVIDEAPRAGGQIYRQP
                     PADAGFARSKRSLYGMEAGKADAVHSTMAGLLSKLDYRPDTLVWGCGAAQLDTLHAGR
                     QRTVPYSHLLIATGATDRVLPFPGWTLPGVYTLGAAQIALKSHGCSIGRRVVLAGTGP
                     LLYLVAYQYAKAGAQVEAVLDTNPFSRQFAATPRLLRQPSIFAKGLYYVGWLAARGVR
                     IERGVTLVRATGDKAVQAMTYRDGEQELTIPCDAVGFGYGLRSETQLADLAGCRFRFD
                     PLNRAWLPERDCAGRSSVPGVYLAGDGAGIAGADAAELAGRRAALAMLEDLGHRVENA
                     DVQAQAAKLDRQLANIAEFRIGLEQAFAPPAGCASHWPDDMTVCRCEEIDAGTLRRCI
                     RGGEASEINRLKALTRVGMGRCQGRMCGAAAAELLSAETGKPISDVGRLRGQAPIKPV
                     PLSIAMMDEVSEADHLAAIPEHARDD"
     gene            1997..3130
                     /locus_tag="VHV99_26475"
     CDS             1997..3130
                     /locus_tag="VHV99_26475"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013342648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX3638965.1"
                     /translation="MTETLRFDVLVVGGGLVGSSTALMLAKRGLKVGLFERRYCGAQA
                     SGVNYGGVRRQGRPAEQLPLAVRARSIWDQLPELVGTDGEFVVSGHLRLARSEADLAA
                     LEAYAQVANPFGLKLQLMSGSTFRRRYPWLGAAAVGGSLCPGDGHANPRVVSPAFARA
                     ARAAGADVREQTMVTNIVRAGDRFVVEARHDSGAAIEVRSDWLVNAAGAWGNTVAGYF
                     GETIPMEPIYPNMWVTEPLPYFIDHNLGVYGGGIYARQVARGNVVIGGGRGIGDGEYA
                     QPLTETTRTVMRAACALLPALKDALLLRTWTGVEGSTPDDNPVIGFSRTTPRLLHAFG
                     FSGGGFLLAPGVGAVLSDLVVEGESGTPIEAFSIGRFGNGLGC"
BASE COUNT          589 a          949 c         1238 g          768 t
ORIGIN      
        1 cgcggaccat cacgttgcct gtgcgcatgt acatgtacgc aaccgaatcg atcgacccga
       61 tgatggcgtc ggtatcggcg ctcgtgatct tcatcacggc cggggcgatg atcgtgctcg
      121 accgcgtgta tggactgaac cggatcctga tcggttcaca ttgattcgct cggcccccac
      181 gaattcactc tacgcgatga caaaccttcc cagcgcaaca tccaccggac agccgcatac
      241 cgcaaccgat gccgcaccgc aattcgtgcg tcttacggaa gcgcatcgca agcaagtgac
      301 gttgacgctg gatggtgttg cgatcacagc cttaactggt gatacgttgc tgagtgccat
      361 cctgctgcac acacgacggg tgcgcgacac cgagttcagt ggcgaaccgc gtgcgggatt
      421 ctgtctgatg ggcgcgtgcc aggattgctg ggtgcaactg gaagagggca cacgaatcag
      481 ggcgtgctcg acgttcgtcg aagaagggct gcgtgtcatg acccggcctg ccggagcgac
      541 cggaacgtga gtacgacgat cgataaaaaa agcatgcatc gggtcgtcat cgcaggcgcg
      601 ggaccggccg gcgtgcgtgc ggcccagacg ttggtcgcgg cggggattgt gccggttgtg
      661 atcgatgaag cgccgcgtgc gggtggccag atctatcgcc agccgccggc cgatgcaggt
      721 tttgcccgca gcaaacggtc gctgtatggc atggaagcag gcaaggccga tgcagtgcat
      781 tcgaccatgg cagggttgct ttccaaactc gactatcggc cggacacgct ggtgtgggga
      841 tgcggcgccg cgcagctcga taccttgcat gccggacgcc agcgcacggt gccttattcg
      901 cacttgctga ttgcgaccgg cgcgacggat cgcgtgctgc cgtttccggg ctggacgctg
      961 ccgggcgtct acacgcttgg cgctgcgcag attgcgctga aatcgcatgg ctgttcgatt
     1021 ggacgtcgcg tggtgttggc gggaaccggg ccgttgcttt atctcgtggc gtatcaatac
     1081 gcgaaggcgg gcgcgcaggt ggaggcggtg ctcgacacca atccattctc tcgtcagttc
     1141 gccgcgacgc cgcgattgtt gcggcagcca tccatcttcg caaagggcct ctattacgtc
     1201 ggctggctgg cggcgcgcgg ggttcgtatc gaacgtggcg tcacgctcgt gcgcgccacg
     1261 ggcgacaaag ccgtgcaggc gatgacctat cgcgatggcg aacaagagct gaccattccc
     1321 tgcgatgcgg tcgggtttgg ctatggcctg cgttctgaga cgcagcttgc cgatctggcg
     1381 ggctgccgtt ttcgcttcga tccgctcaat cgcgcgtggc tgccggagcg cgattgcgcc
     1441 ggtcgcagtt cggtgccggg cgtttatctc gccggcgatg gcgcgggtat cgccggtgcg
     1501 gatgctgcgg aactggctgg ccgtcgcgcg gcgctcgcga tgctggaaga cctggggcat
     1561 cgggttgaga acgctgacgt tcaggctcag gctgcgaagc tggaccggca actggcgaat
     1621 atcgctgagt ttcgtattgg gttggagcag gcgtttgcgc cgcccgccgg ttgcgcgtcg
     1681 cactggccgg acgacatgac ggtgtgccgt tgcgaggaga tcgatgccgg cacgctgcgc
     1741 cgttgtattc gaggaggcga agctagtgag atcaaccggc tgaaggcgct tacgcgtgtg
     1801 ggcatgggcc gttgccaggg acgcatgtgc ggcgcggcag cggcggaatt gctgagcgcg
     1861 gagacgggca aaccgatctc ggatgtcgga cgtttgcgcg gacaggcgcc gatcaaaccc
     1921 gtgcctttat cgatagcgat gatggatgag gtatccgaag cggatcatct tgccgccatc
     1981 ccggagcatg cacgtgatga ctgagacttt gcggtttgat gtgctggtcg tgggcggcgg
     2041 gttggtggga tcatcaacag ccttgatgct ggcaaagcgc gggcttaagg tcggactttt
     2101 tgaacgccga tattgcggcg cgcaggcgag cggtgtgaac tacggcggtg tgcgccgtca
     2161 ggggcggccg gccgaacaat taccacttgc ggtgcgtgca cgatcgatct gggaccagtt
     2221 gccggaactc gtgggcaccg atggagagtt cgttgtatcg gggcatttgc ggctggctcg
     2281 cagcgaggcg gatcttgcgg cgctcgaagc gtacgcgcag gttgccaatc cattcggact
     2341 taagttgcaa ctgatgagcg gatctacgtt caggcgtcgt tacccgtggc ttggggcggc
     2401 tgcggtgggt ggctcgttgt gtcccggtga cggtcatgca aatccacgcg tggtatcgcc
     2461 cgcgttcgcg cgggcggcgc gtgcagccgg tgctgatgtc cgtgagcaga ctatggtcac
     2521 gaacatcgtg cgtgcggggg atcggtttgt tgtcgaggcg cggcatgact cgggtgctgc
     2581 gatcgaggtc cggagcgact ggctggtcaa tgctgccggc gcgtggggca acacggtggc
     2641 cgggtacttt ggcgagacca ttccaatgga gccgatctat ccgaacatgt gggtgactga
     2701 gccgctgccc tatttcatcg atcacaatct tggggtttat ggcggcggca tttatgcccg
     2761 tcaggtggcg cgtggcaatg tggtgattgg tggcggtcgt gggattgggg atggtgagta
     2821 tgcccagccg ctcacggaga ctacgcggac ggtgatgcgt gcggcgtgtg cgcttttgcc
     2881 agcgttgaaa gatgcgttgt tgttgcggac ctggaccggt gtggagggca gtacacctga
     2941 cgataacccg gtgatcggtt ttagccggac tacgccaagg ttgctgcacg catttggatt
     3001 ttcggggggc gggtttttgc ttgcgccagg ggttggagcg gtgctttctg acctggtcgt
     3061 tgagggggaa tcggggacgc cgattgaggc gttttctatt ggtcggtttg ggaacggttt
     3121 ggggtgctag gttttggggt tagcggtcgg ggtcaatgcg aggtttcggg gttagcggtt
     3181 ctgggtcaat gccgggttgc tgctttcggt gtttgtggtc tgcgtgagtt ggctaatttc
     3241 tttatcactg ttagccactg tgcgtggcgg cacgtcattt ctttgtccaa agcgacaaag
     3301 aaacgaagca aagaaagcgc tttcaaccac gctaccctaa gtgtccacag cgtgcagttt
     3361 ctattcatag gtgccccaaa agcacggtgc tcgccagagc cacggatgtg tgaacccctc
     3421 cttctcgcga atcctgacac gaacacgctt cgccccgtac gctctcgggc aagcaccagt
     3481 gttccacgga cccgctcggc ctaacgcatt tttgagacct tgcttcttcg tgggttggcg
     3541 acgt
//
LOCUS       DATASH010000993         2794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4522975,
            whole genome shotgun sequence.
ACCESSION   DATASH010000993 DATASH010000000
VERSION     DATASH010000993.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4522975"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..994
                     /gene="argF"
                     /locus_tag="VHV99_26480"
     CDS             65..994
                     /gene="argF"
                     /locus_tag="VHV99_26480"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004190177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX3638966.1"
                     /translation="MTAKKIRHYLQFKDFSLEDYEYVLERARILKRKFKNYETYHPLH
                     DRTLAMIFEKNSTRTRLSFEAGIFQLGGHAVFMSTRDTQLGRGEPIEDAAQVISRMVD
                     IIMIRTFGQDILTRFAENSRVPVINGLTNEYHPCQVLADIFTYFEHRGPIRDKTVAWV
                     GDANNMLYTWIEAAQILGFKLRLSTPPGYKLDRAMVAAESAPFYQEFDDPNEACTGAD
                     LVTTDVWTSMGFEAENEARKKAFADWCVDAYMMARANSDALFMHCLPAHRGEEVSAEV
                     IDGPQSVVWDEAENRLHVQKALMEYLLLGKLNH"
     gene            1232..2458
                     /locus_tag="VHV99_26485"
     CDS             1232..2458
                     /locus_tag="VHV99_26485"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEX3638967.1"
                     /translation="MSDIKKVVLAYSGGLDTSVILKWLQDNYDAEVVTFTADIGQGEE
                     LEPARKKALQLGIKQENIFIEDLREEFVRDYVFPMFRANTIYEGEYLLGTSIARPLIA
                     RRQIEIARATGAQAVSHGATGKGNDQVRFELGYYALEPGIKVIAPWREWDLLSREKLL
                     TYAEKAGIPIELKHKQGGAPYSMDANLLHISFEGRHLEDPKAEAEADMWRWTVSPEQA
                     PDQAEYLDIEYEHGDPVAINGKRLSAAEMLTELNRLGGKHGIGRLDLVENRYVGMKSR
                     GCYETPGGTIMLKAHRGIESITLDREVAHLKDDLMARYASLIYNGYWWSPERRAIQVL
                     IDHTQEKVNGWVRVKLYKGSVSVVARDSKETLFDKTIATFDDDGGAYNQADAGGFIKL
                     NALRMRIAENARRQRS"
BASE COUNT          627 a          865 c          823 g          479 t
ORIGIN      
        1 gttaccacaa acgacggcgg cgccgtccgg caatcctgcc gggtgagacc gccgtatttg
       61 tttcatgacc gccaagaaaa ttcgccacta cctgcagttc aaggatttct cgctggaaga
      121 ctacgagtac gtgctggaac gcgcgcgcat tctgaaacgc aaattcaaga actacgagac
      181 ttatcacccg ctgcacgacc gcacgctggc gatgatcttc gagaaaaact cgactcgcac
      241 gcgcctctcg ttcgaagccg gcatcttcca gctgggcggc cacgccgtct tcatgagcac
      301 gcgcgacacg cagctcggcc gcggcgaacc gattgaagac gcagcgcaag tgatctcgcg
      361 catggtcgac atcatcatga tccgcacgtt cggccaggac attctcacgc gctttgccga
      421 gaattcgcgc gtaccggtga tcaacgggct gaccaacgaa taccacccgt gccaggtgct
      481 ggcggacatc ttcacgtact tcgagcatcg cggtccgatt cgcgacaaga cggtcgcgtg
      541 ggtcggtgac gcgaacaaca tgctgtacac gtggatcgaa gccgcccaga ttctcggttt
      601 caagctgcgc ctgtccacac cgccgggcta caagctcgac cgcgccatgg tcgcggctga
      661 aagcgcaccg ttttaccagg aattcgacga tccgaacgaa gcctgcacag gcgccgacct
      721 ggtcaccacg gacgtgtgga ccagcatggg cttcgaggcc gaaaacgaag cacgcaagaa
      781 agccttcgca gactggtgcg tggacgccta catgatggcg cgtgccaatt ccgatgcatt
      841 gttcatgcac tgcctgcccg cccaccgcgg cgaggaagtc agcgcggaag tgatcgacgg
      901 cccgcaaagc gtcgtgtggg acgaagcgga aaatcgtctg cacgtgcaaa aggcattgat
      961 ggaatatctg ctgctcggca agctgaatca ttaagcaggc cacgtgcagc acctgcgtga
     1021 agcgcgacgg ggccttgaat acgaggcaag gcatccacgt ttagcgccac gcatcgagcc
     1081 gcggtccacc gctacgcgcg gacccggcca tgtcacctca ctgccccaaa aacgcgggca
     1141 ggtggtggtt tagtgtcaaa atagtggttt tccgcgctcc ggaaccgccg gcgcgccttg
     1201 ttttcccgct ttctccagct acgagttcat catgagcgat atcaagaaag tcgtgctcgc
     1261 ctattcgggc ggcctcgaca cctccgtcat cctgaagtgg ttgcaagaca attacgacgc
     1321 cgaagtcgtc acgtttacgg ccgacatcgg ccagggcgaa gagctggaac cggcgcgcaa
     1381 gaaagccctg caactcggca tcaagcagga aaacatcttc attgaagacc tgcgcgaaga
     1441 attcgtgcgc gactacgtgt tcccgatgtt ccgcgccaac acgatctatg aaggcgaata
     1501 cctgctgggc acgtcgattg cccgtccgtt gatcgccagg cgtcagatcg aaatcgcccg
     1561 cgccaccggc gcgcaagcgg tgtcgcacgg cgcgaccggc aagggcaacg accaggttcg
     1621 cttcgaactc ggttactacg cgctggaacc gggcatcaag gtgatcgcac cgtggcgtga
     1681 atgggatctg ctgtcgcgcg aaaagctgct cacgtacgct gaaaaagccg gcattccgat
     1741 cgaactgaag cacaagcaag gcggcgcgcc gtattcgatg gacgcgaacc tgctgcacat
     1801 ctcgttcgaa ggccgtcacc tggaagaccc gaaggccgaa gccgaagccg atatgtggcg
     1861 ttggacggtg tcgccggaac aggcgcccga ccaggctgaa tacctcgaca tcgagtacga
     1921 gcacggcgat ccggtcgcga tcaacggcaa acgtctgtcg gcggctgaaa tgctgaccga
     1981 gttgaaccgt ttgggcggca agcacggcat cggccgtctg gatctggtcg aaaaccgtta
     2041 cgtcggcatg aagtcgcgcg gctgctatga aacgccgggc ggcacgatca tgctgaaggc
     2101 gcaccgcggc atcgagtcga tcacgctcga ccgcgaagtg gctcacctga aagacgatct
     2161 gatggcgcgc tacgcctcgc taatttacaa cggctactgg tggagcccgg agcgccgcgc
     2221 aatccaggtg ctgatcgacc acacgcaaga gaaggtcaac ggctgggtgc gggtgaagct
     2281 gtacaagggc agcgtgtcgg ttgtcgcgcg cgattcgaag gaaacgctgt tcgacaagac
     2341 catcgctacg ttcgacgacg acggcggtgc ttacaatcaa gccgacgctg gcgggttcat
     2401 caagctgaat gcattgcgca tgcggatcgc ggagaacgca cgtcgtcagc gtagctgagg
     2461 gcttgcgttt cggtgaaggc taacttcgcg acagcgaagt taagcgccag gaagcgcggc
     2521 cgacaccgaa gcgcagacgc aaaaaagggc gcccggagga aactcccggc gcccttttca
     2581 attgctacgt tgcacttatc cgctcacgaa gaacctgatc cgcattcagt ttcaaaccgc
     2641 gtatggcagc gcgaccacca actcccccgc gcagatcgcc gcatcgtcat tgaccgacgc
     2701 cagcgactgc cgtgtcacaa gcaaaccggc tccgcttcca acgccacgcc aaaccgcgcc
     2761 aacacatccc gctgaaccgc tttcgcgagc gcca
//
LOCUS       DATASH010000994         2405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6300519,
            whole genome shotgun sequence.
ACCESSION   DATASH010000994 DATASH010000000
VERSION     DATASH010000994.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6300519"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..423
                     /locus_tag="VHV99_26490"
     CDS             235..423
                     /locus_tag="VHV99_26490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638968.1"
                     /translation="MPALIKNQLYRPSVVLPMVALMQVMVSQDFNLGQVGWVVAARGA
                     QAALQRSRELICAAHCHA"
     gene            516..1916
                     /locus_tag="VHV99_26495"
     CDS             516..1916
                     /locus_tag="VHV99_26495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEX3638969.1"
                     /translation="MPQHFDAVVIGTGQGGAPLAVRLGQSGRKTAVIERAAFGGTCVN
                     VGCTPTKSYVASARAAHVARHAAELGVQVSGSVTVNLAAVKARKDEIIGESRDGVEKW
                     LRGADNVTVFNGHARFTGAHTLSISGPDGTVREDISADEIFINTGTRAVVPPLEGLER
                     IRYYTNSNLLELTELPDHLAIVGGSYIALEFAQIYRRFGSRVTVLVRGERVLTREDAD
                     FAKSVQKVLAREGIEFVFGVQPSRVEPHPHRENAVCIGFEQNIPVLEASHLLFATGRE
                     PNTDDLGLAAAGIATDRHGTIPVDGQLRTNVPGIWAIGDINGRGAFTHTSYDDFHIVA
                     ANLLDGGSRSVDTRIMAYAVFVDPPLARVGLSEADVRKTGRDARIATMPMTRVGRARE
                     RGETDGFMKVLVDAHSKQILGAAIHGIEGDEAIHTFIDLMTAGAPYPTLQYAMHIHPT
                     ISELVPTLLDGVKPMK"
     gene            2054..2401
                     /locus_tag="VHV99_26500"
     CDS             2054..2401
                     /locus_tag="VHV99_26500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX3638970.1"
                     /translation="MKRAINSGNLFDFGAPKSSGEQLDSLAEQQGVSIERIVSRGHAS
                     PPDFWYDSPRAEWVVLLSGAATLEFESDPQPHPMKPGDHVLIEAHCRHRVAWTSGTEP
                     SVWLAVYYPGVRR"
BASE COUNT          467 a          767 c          779 g          392 t
ORIGIN      
        1 tcctgatgct gtcggccatg gccaccgccg caggcgcggc aaaaccactt cgccagacgg
       61 gctaatacaa catcggactc cgttgccgcg ggcgcccaga ctcgattgtt cagatcggct
      121 gacaagtgcc ttcgcgttcc cggtatttat ccgcgcccca agtctcccta gaattctccc
      181 atcgaatgta aattccgcgc catgcctaag cggcccggcg cgacgggagc cgttatgcca
      241 gccttgatca aaaaccagct ttatcggcca tcggtggtcc tgccgatggt cgccctcatg
      301 caagtgatgg tgtcgcagga cttcaacctc ggccaggtcg gctgggtcgt ggcagcgcgc
      361 ggcgcgcagg ccgcactgca acgctcgcgc gaattgatct gcgcggcgca ctgccacgcc
      421 tgaacgacgc aaactcctag aaacgacgat cacggcaacc caaggcgctg agggtaagat
      481 gctacgacca gcaccccagc ctcgggaggt ttgccatgcc acagcacttc gacgccgtcg
      541 tgatcggcac gggacaaggc ggtgcgccgc tcgctgtgcg tctcggtcaa agcggacgta
      601 aaaccgcggt catcgaacgc gcggccttcg gcgggacctg tgtgaatgtc ggctgcacgc
      661 cgaccaagtc gtatgtggcc agcgcccgcg cggcccacgt ggcgcgccac gccgccgagc
      721 tgggagtgca ggtcagcggc tcggtcaccg tcaatctggc ggcggtcaag gcgcgcaagg
      781 acgagatcat cggcgaatcg cgtgacggcg tcgagaaatg gctgcgcggc gcggataacg
      841 tgaccgtctt caatggccac gcgcgcttca ccggcgcgca tacgttgtcc atcagcgggc
      901 cggacggtac ggtgcgcgag gacatcagcg ccgacgaaat cttcatcaat accggcacgc
      961 gcgcagtcgt gccgccgctc gaaggacttg agcgaattcg ttactacacc aactccaatc
     1021 tgcttgaact gacggaactg ccggaccatc tggcgattgt cggcggcagc tatatcgcgc
     1081 tcgaattcgc gcagatttat cgccgcttcg gcagccgggt gacggtgctg gtacgcggcg
     1141 agcgcgtgct cacgcgcgag gatgccgact tcgccaagtc cgtgcagaag gtgctggcac
     1201 gtgaagggat cgagtttgtt ttcggcgtgc agccttcgcg ggtcgaaccg catccgcatc
     1261 gcgagaacgc ggtgtgtatc ggcttcgagc agaacattcc cgtgctcgaa gcctcgcacc
     1321 tgctgttcgc caccggccgg gaaccgaata ccgacgacct cggcctcgcc gccgccggca
     1381 tcgcgacgga caggcacggc acgattcccg tcgacggcca gttgcgcacc aacgtgccgg
     1441 gcatctgggc gatcggcgac atcaacggac gcggcgcctt tacccacacg tcctacgacg
     1501 acttccacat cgtcgcggcc aatctgctcg acggcggctc gcgcagcgtc gacacgcgga
     1561 tcatggcgta tgccgtgttc gtcgatccgc cgctcgcgcg cgtcgggctt tcggaggccg
     1621 atgtacgcaa gaccggccgc gacgcgcgga tcgccaccat gccgatgacg cgggtgggcc
     1681 gtgcccgcga acgcggcgag accgacggct tcatgaaggt gctggtcgac gcccacagca
     1741 agcagattct tggcgcggcg atccatggca tcgaaggcga tgaagccatc catacgttta
     1801 tcgacctcat gacggccggg gcgccttatc cgaccttgca gtacgcgatg cacattcatc
     1861 cgaccatcag cgaactggtg ccgaccttgc tcgatggggt gaagccgatg aagtgagcgc
     1921 gcgagcggca agcgcgaggg acgaacgagc gctgcggacg agcgcaagac gaacgccgct
     1981 acgagtgcaa cgaagagtgc aaggaacgcg agcaaagaac gcgagcaaag aacgagtgcc
     2041 aggagcacag gccatgaaac gcgcgatcaa cagcggcaat ctgttcgatt tcggtgcacc
     2101 gaagagctcc ggcgagcaac tcgattctct ggcagagcaa cagggcgtga gcatcgagcg
     2161 gatcgtctcg agggggcacg cgagcccgcc ggacttctgg tacgacagtc cgcgggccga
     2221 gtgggtcgtg ttgctgagcg gcgcggcaac gctcgaattc gaaagcgatc cgcagcctca
     2281 tccgatgaag ccgggcgacc acgtgctgat cgaagcgcat tgccggcatc gggtggcatg
     2341 gacgagcggc acggagccga gcgtgtggct agcggtttac tatcctggcg ttcgccgctg
     2401 attga
//
LOCUS       DATASH010000995         1804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3729939,
            whole genome shotgun sequence.
ACCESSION   DATASH010000995 DATASH010000000
VERSION     DATASH010000995.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3729939"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..257
                     /locus_tag="VHV99_26505"
     CDS             12..257
                     /locus_tag="VHV99_26505"
                     /inference="COORDINATES: protein motif:HMM:NF042466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase beta-barrel
                     domain-containing protein"
                     /protein_id="HEX3638971.1"
                     /translation="MHWLIDALAAPFRCTVKVRYRQVDQAAVFEPRSDGTARILFDDP
                     QRAVTPGKYAVAYENDRCLGGAVIESVNSASEQQAAA"
     gene            390..>1804
                     /gene="acnA"
                     /locus_tag="VHV99_26510"
     CDS             390..>1804
                     /gene="acnA"
                     /locus_tag="VHV99_26510"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003805666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEX3638972.1"
                     /translation="MTNSFNARTTLNSGGRSYDIWSLAALPKEKVARLPYSLKILLEN
                     LLRFEDGVNVTRADIDALLSWDPAAVPSYEIAFTPARVILQDFTGVPCVVDLAAMRDA
                     IVKLGGDAERVNPLAPAELVIDHSVQVDEYGTPGSLAANNKIEFARNGERYSFLRWGQ
                     TAFRNFQVVPPNTGIVHQVNLEYLGRVIFDGEVNGKPRAYPDTLVGTDSHTTMINGLG
                     VLGWGVGGIEAEAAMLGQPVTMLIPQVIGFRLNGKLKPGATATDLVLTVTEMLRKKGV
                     VDKFVEFFGDGLANLPLADRATIANMAPEYGSTCGIFPIDEETIRYLELSGRPRERID
                     LVAAYAKAQGMWRNNGDKPADYTDVLELDLSTVEPSLAGPRRPQDRVPLQNAKSVYET
                     NGKKTSDERAVKNAGAKGVATATLDGKSFELKDGAVLIAAITSCTNTSNPSVMLGAGL
                     LARKALAKGLTSKPWVKTSLAP"
BASE COUNT          365 a          565 c          539 g          335 t
ORIGIN      
        1 ctacggggcc tatgcattgg ctcatcgacg ccctggccgc accattccga tgcacagtga
       61 aggtacgtta ccggcaagta gaccaggccg cggtattcga gcccaggagt gatggcacgg
      121 cgcggattct gttcgatgac ccacagcgcg cagttacgcc cggtaagtac gctgttgcgt
      181 acgaaaatga ccgctgtctc ggcggtgctg tgattgagtc ggtgaattca gcgtcagagc
      241 aacaggcggc ggcgtagcga aatctatata attcgccgcg ctattttcct attggcggaa
      301 attgcctgcg gcaatttccg cagacatcag ggtcgaccgc cggcaaaaag cgtggttttt
      361 gccggctgct cagtggacgt ggagaattca tgactaacag ctttaacgcg cgaacgacgc
      421 tcaacagcgg cggtcgctct tacgatatct ggagcctcgc cgctcttcct aaagaaaagg
      481 ttgcgcgact gccctactcc ctcaagatcc tgctcgagaa cctgctgcgc ttcgaagacg
      541 gtgtgaacgt cacacgtgct gacatcgatg cgctgctttc gtgggatcca gccgccgtac
      601 cttcgtacga gatcgccttc acgcccgccc gcgtgattct tcaggacttc actggcgtgc
      661 cttgcgtcgt cgatctagca gcaatgcgcg atgcgatcgt aaagctcggt ggcgatgcgg
      721 agcgtgtcaa tccgctcgcg cccgccgaac tcgtcatcga tcactcggtg caagtcgatg
      781 aatacggaac gccgggctcc ctcgcggcca acaacaagat cgagtttgcc cgcaacggcg
      841 agcgttattc gttcctgcgc tggggccaga ccgccttccg caatttccag gtcgttcccc
      901 cgaacaccgg catcgtgcac caggtgaacc tggaatacct gggccgagtt attttcgacg
      961 gtgaagtcaa cggcaagccg cgcgcttatc cggacacgct ggtcggcacc gactcgcaca
     1021 cgaccatgat caacggcctc ggcgtgttgg gctggggtgt cggcggcatc gaggctgaag
     1081 cggccatgct cggccagccc gtgaccatgt tgattccgca ggtcatcggc ttccgcctga
     1141 acggcaaact caagcccggc gccaccgcga ccgatctcgt gttaaccgtg accgaaatgc
     1201 tgcgcaagaa aggggtcgtg gacaagttcg tcgaattctt cggcgacggg ctcgcgaacc
     1261 tgccgctcgc cgatcgcgcg acgattgcca acatggcgcc ggagtacggc agcacgtgcg
     1321 gcattttccc gatcgacgaa gagacgattc gttatctcga gctgtcgggc cgccctcgtg
     1381 agcgaatcga cctggtcgcc gcctacgcga aggcccaggg aatgtggcgc aacaacggcg
     1441 acaagcctgc agactacacc gacgtgttgg aactcgacct cagcacggta gagcccagcc
     1501 tcgccggtcc gcgtcgcccg caggaccgcg taccgctgca gaatgccaag tccgtatacg
     1561 agacgaacgg caagaagact tccgatgagc gcgcggtcaa gaatgccggc gccaagggtg
     1621 ttgcaacagc gaccctcgac ggcaagagct tcgaactgaa ggacggcgcg gtgctgatcg
     1681 cagccatcac cagctgcacc aacacgtcca atccgtcggt gatgctcggt gccggactgc
     1741 tggctcgcaa ggcgctcgcc aagggactca cctccaagcc gtgggtgaag accagcctcg
     1801 ctcc
//
LOCUS       DATASH010000996         4962 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6895514,
            whole genome shotgun sequence.
ACCESSION   DATASH010000996 DATASH010000000
VERSION     DATASH010000996.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4962
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6895514"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /gene="hrcA"
                     /locus_tag="VHV99_26515"
     CDS             <1..850
                     /gene="hrcA"
                     /locus_tag="VHV99_26515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEX3638973.1"
                     /translation="PHTSAGRIPTPRGYRLFVDTMLTVESAADEEAVMRTVKTTLQAG
                     EPQKIVAAAASVLSNLSQFAGVVLTPRRSHVFKQIEFMRLSDKRILLIIVTPEGDVQN
                     RIMATQRDFSPSQLVEASNYINAHFAGLSFDDVRRRLREEIDELRGDMTTLMHAAVTA
                     STDESDTGETVLISGERNLLEVADLSSDMARLRKLFDVFDQKTSLLQLLDVSSHAAGV
                     QIFIGGESNLVPIEEMSVVTAPYEVNGKIVGTLGVIGPTRMAYNRVIPIVDITARLLS
                     LTLSQQ"
     gene            1027..2097
                     /gene="hemH"
                     /locus_tag="VHV99_26520"
     CDS             1027..2097
                     /gene="hemH"
                     /locus_tag="VHV99_26520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEX3638974.1"
                     /translation="MRFDLERPSQNATSHRVAVLLINLGTPDAPTPRAVRRYLAQFLS
                     DPRVVEIPALLWQVILRLLILPFRGRASAKKYAAVWMPEGSPLRVFTEKQVEGLRHLL
                     QLNDYTVLVDYAMRYGTPGIPAMLNQLKLAGVERVLLMPMYPQYSSSTTATAFDDAFS
                     ALKRMRNQPEIRTVRQYADHPAYIAALAAQVHQYWHQHGKPDFAAGDKLVLSFHGVPK
                     RTLDLGDPYHEQCQQTGALLMQALGLSQVECRITFQSRFGKAEWLQPYTAPTLKELGS
                     AGVRRADVFCPGFTADCLETIEEIGMEVRDEFVHAGGKEFHRIPCLNASQPWIAALGE
                     IVAQNLQGWPVQAVSVPHTSGA"
     gene            2106..2513
                     /locus_tag="VHV99_26525"
     CDS             2106..2513
                     /locus_tag="VHV99_26525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015001663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEX3638975.1"
                     /translation="MNYRIATDPGAKLRIDKWLWAARFFKTRSLAADAVEKGHVRIGG
                     ASVKPAKDVRVGDLVEVEIERMVWQVEVLGVCDVRGPASVAQTLYTETEESRVKRQVE
                     QERRKTYREPAAALHGRPTKRDRRTIDKLSGGD"
     gene            complement(2485..2646)
                     /locus_tag="VHV99_26530"
     CDS             complement(2485..2646)
                     /locus_tag="VHV99_26530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3638976.1"
                     /translation="MKLTFAISVVALVLIAGTTTICMSGAVNDHTTEYGGVHATMEQL
                     FNPHLKVCR"
     gene            3023..3607
                     /gene="grpE"
                     /locus_tag="VHV99_26535"
     CDS             3023..3607
                     /gene="grpE"
                     /locus_tag="VHV99_26535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013338097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX3638977.1"
                     /translation="MENTQENPTSQNPTAADETARQAAEAAAPQQEAAANAAPEAPAT
                     GAEAALAEAEAKIAELQESFLRAKAETENVRRRAQEDVAKAHKFAIESFAEHLLPVID
                     SLEAAVAHSSDDLQKVREGVELTLRQLTGALEKGRVVALNPVGEKFDPHRHQAISMVP
                     ADQDPNTVVAVLQKGFVIAERVLRPALVTVAAPK"
     gene            3721..4139
                     /locus_tag="VHV99_26540"
                     /pseudo
     CDS             3721..4139
                     /locus_tag="VHV99_26540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069709.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
     gene            4293..>4962
                     /locus_tag="VHV99_26545"
     CDS             4293..>4962
                     /locus_tag="VHV99_26545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX3638978.1"
                     /translation="MGKIIGIDLGTTNSCVAIMEGNSVKVIENSEGARTTPSIIAYME
                     DGEILVGAPAKRQSVTNPRNTLYAVKRLIGRRFEEKEVQKDIGLMPYKIMKADNGDAW
                     VEVRDQKLAPPQISAEVLRKMKKTAEDYLGEPVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLEVKRIINEPTAAALAFGLDRKESRDGKIAVFDLGGGTFDISIIEIADVHGEKQFE
                     VLSTN"
BASE COUNT         1025 a         1467 c         1553 g          917 t
ORIGIN      
        1 tccgcatact tcagcgggcc ggatcccgac gccgcgcggc taccgcctgt tcgtcgatac
       61 catgctgacg gtggaatccg ccgcggacga ggaagcggtg atgcgcaccg tcaagactac
      121 gctgcaggcg ggcgaaccac agaaaatcgt cgccgcggcg gccagcgtgc tgtccaatct
      181 gtcgcagttc gccggtgtcg tgctcacccc gcgccgcagc cacgtgttca agcagatcga
      241 attcatgcgc ctgtcggaca agcgcatcct gctgatcatc gtgacgcccg agggcgacgt
      301 gcagaaccgt atcatggcca cccagcgcga cttctcgccg tcgcaactgg tggaagcctc
      361 caattacatc aacgcgcatt tcgccggcct ctcgttcgac gacgtgcgcc gccgcctgcg
      421 cgaagaaatc gacgaattgc gtggcgacat gaccacgctg atgcatgccg cggtcacggc
      481 cagcactgat gaatctgaca ccggcgagac cgtgctgatt tccggcgagc gtaacctgct
      541 cgaagttgcc gacctttcgt cggacatggc gcgcttgcgc aagctgttcg atgtgttcga
      601 ccaaaagacc agtctccttc aattgctcga cgtgtcgagt cacgccgcgg gcgtgcagat
      661 ttttattggc ggcgagtcga atctcgtccc gatcgaggag atgagcgtgg tgaccgcgcc
      721 atatgaggta aacggcaaga tcgtcggcac gctcggcgtg atcgggccga cccgcatggc
      781 ctataaccgc gtgattccga tcgtggatat caccgcgcgg ctgctttccc tgaccctcag
      841 ccagcagtaa cacctgcggt cacggcgggc tgcggccgct gatgttgcaa atgatgcgcg
      901 acggcgcgcg gcttgccaat tggccgaatg gccaggggtc cctggcgaac ccgcaatgga
      961 ccgcgccgct ataatgaatc ccaccttgaa tcaactttcc gcctaaagcg gttatgcctg
     1021 ctgcctatgc gctttgacct cgagcggccc tcgcagaacg ccacgtcaca ccgtgtcgcg
     1081 gtgctgctga tcaatctcgg cacgcccgac gcgccgacgc cgcgcgcggt ccgtcgctat
     1141 ctcgcgcagt ttctgtccga tcctcgcgtg gtcgaaattc cagcgctgct ctggcaggtg
     1201 atcctgcggc tgttgatttt gccgttccgg ggccgcgcgt cagcgaagaa gtacgcagcc
     1261 gtgtggatgc cggaaggctc gccgctacgc gtcttcacgg aaaagcaggt agaaggcttg
     1321 cgccatctgc tgcagttgaa cgactacacg gtgctggtcg actacgcgat gcgttatggc
     1381 acacccggca ttccggccat gctgaaccag ctcaagctgg cgggcgtcga gcgcgtgctg
     1441 ctgatgccca tgtatccgca atattcctcg tcgaccacgg caacggcctt cgacgatgcc
     1501 ttttcggccc tcaagcgcat gcgcaatcag ccggaaatcc gcaccgtgcg ccagtacgct
     1561 gatcatccgg cgtatatcgc cgcgctcgct gcccaagtcc atcaatactg gcatcaacac
     1621 ggcaagccgg attttgcagc gggcgacaag ctggtgctca gttttcacgg tgtacccaag
     1681 cgcactttgg atctgggcga tccgtaccac gagcaatgcc agcaaacggg cgcgttgttg
     1741 atgcaggcgc tgggattgag ccaggtcgaa tgccgtatca cgtttcagtc gcgcttcggc
     1801 aaggccgaat ggttgcagcc gtacaccgcc cccacgctga aggaacttgg ctcggcgggc
     1861 gtgcggcgtg ccgacgtgtt ctgcccgggc tttaccgccg attgccttga aacgattgaa
     1921 gaaatcggca tggaagtgcg tgacgagttc gtgcatgcgg gcggcaagga atttcaccgg
     1981 attccctgcc tgaacgcctc gcagccgtgg atcgccgcac taggagaaat cgtcgcgcag
     2041 aatctgcagg gctggcccgt gcaagcggtg tcggtgccgc acacgagcgg ggcttaagca
     2101 ggcttatgaa ttacaggatt gcaacggacc cgggcgcgaa gctgcgcatc gacaaatggc
     2161 tttgggcggc acgcttcttc aagacccgct cgctcgcggc ggatgcggtc gagaaaggcc
     2221 atgtgcgcat tggcggggcc agcgtcaagc cggcgaaaga tgtgcgcgtc ggcgacctgg
     2281 tcgaggtcga gatcgagcgg atggtgtggc aggtggaagt gctgggcgtg tgcgacgtgc
     2341 gtggcccagc cagcgtcgcg cagacgcttt atacggaaac cgaagagagc agggtgaagc
     2401 ggcaggtcga gcaggaacgg cgcaagacgt accgcgaacc ggctgccgcc ttacacggca
     2461 ggccgaccaa gcgcgaccgg cgcactatcg acaaactttc aggtggggat tgaacaactg
     2521 ttccatcgtc gcgtgcacac cgccgtactc ggtggtgtga tcgttgacgg ccccggacat
     2581 gcagatggtc gtggtgcccg cgatcagtac caatgcgacc accgatattg caaaggtcag
     2641 tttcacggta ctcccctgga aagattcagg atggaaacgg gcgaatcagg tggaatttga
     2701 aaagtgtcgg taaggcttac gtcagattag ggttaacgtg tcttttccga tgctttattg
     2761 gagcataggc cacttgtagg acgcactcaa tctcattctg attgcgacgc actaatggtt
     2821 gtaacggggc atttctgccg cacgtccttg gtgcgaaaag ttccgggaga gccgcttgaa
     2881 atgggtcttt ccggccccat ctgccgtttc gatacgcatt aagcactgag ttgtctgtcg
     2941 caattttgca acgcacaaat tgcgcgcgcc ttgatgcgtt gccgccactg ttagcctcaa
     3001 ctttttaacg actttcagcg acatggaaaa cacgcaagag aacccgacga gccagaatcc
     3061 cacggccgcc gacgaaacgg cgcgccaggc cgcagaggcc gcagcaccgc agcaggaagc
     3121 ggctgcaaac gctgctcctg aagcgccggc aacgggcgcc gaagctgcac tggccgaagc
     3181 agaagcgaag atcgccgagc tgcaggaaag cttcctgcga gcgaaggctg agaccgagaa
     3241 cgtgcgccgc cgcgcccagg aagatgtcgc caaggcccac aagttcgcga tcgaaagctt
     3301 cgctgagcat ttgctgccgg tgatcgacag cctcgaagcg gcggtcgctc actcgtccga
     3361 cgatctgcag aaggtgcgcg aaggcgtcga actcacgctg cgtcaactca ccggcgcgct
     3421 ggaaaagggc cgcgtcgtcg cactgaatcc ggttggcgag aagttcgacc cgcaccgtca
     3481 ccaggctatt tcgatggtgc cggcggatca ggacccgaac accgtcgttg cggtgctgca
     3541 aaaaggcttt gtgatcgccg agcgcgtgct gcgtccggcc ctcgtaaccg tcgcggcgcc
     3601 gaagtaagca gcccaagcgg aaatttagaa aagaagccgt ttataagccg tttcgcctga
     3661 caggcgtcgt cgtcgtggcc aacattaccc gtcgtcactg ccgcgtttgc tgtcttcggc
     3721 atgcaggcgc tcagcgctga gctgttcggc accgtgctgg ctgaagtcgg gatgagttgg
     3781 acatggtgtt tttctggggc ccagctgctt taaccgcgag atcgccagga agagggcgac
     3841 gctggctccg gccggccgcg atccgtgcgc tcggtttcag atggttttac agcaacgtct
     3901 acgcgcatcg caaaccaggc cggcgtttcg tgtttcacgg cgtggccacc cgtgcttctt
     3961 ttgccgtggc gagtggcgcc gtgacaagct gctgggccgc gcaaccggct ggcatggcgc
     4021 tgaatggttc gaggcggcgg tagcagcggc gcgggcaccg attcggggcg acccgggcgg
     4081 ccaatgcggc cggcatcgtg acgaacggtg aaaaaagcgg caaaagttcg gttaattgaa
     4141 aaaaattaaa gaccgcatcg taaaagaggt cttgaaaacg atgcggacgc acttatgttg
     4201 agtgcaggta tgaattgaga gcggatcgcc tgaccgccga atgggcaaac agggcgattc
     4261 ccgcagcgat caaagttagg agattagtga aaatgggcaa aatcatcggc atcgacctcg
     4321 gcacgaccaa ctcgtgcgtg gcgatcatgg aaggcaattc ggtcaaggtg atcgagaact
     4381 cggaaggtgc gcgcaccacg ccgtcgatca tcgcttacat ggaagacggc gagatcctcg
     4441 tcggcgcgcc tgcgaagcgt cagtcggtca cgaacccgcg gaacacgctg tacgcggtca
     4501 agcgcctgat cggccgccgc ttcgaagaaa aagaagtgca gaaagacatt ggcctgatgc
     4561 cgtacaagat catgaaagcc gataacggcg acgcatgggt cgaagtgcgc gatcagaagc
     4621 tggctccgcc gcaaatctcc gcggaagtgc tgcgcaagat gaagaagacc gctgaagact
     4681 acctcggcga gcccgtcacc gaagcggtga ttacggttcc cgcgtacttc aacgacagcc
     4741 agcgccaggc aaccaaggac gctggccgca ttgccggtct ggaagtgaag cggatcatca
     4801 acgaaccgac cgccgccgcg ctcgcgttcg gactggaccg gaaagaaagt cgtgacggca
     4861 agatcgccgt gttcgacctc ggcggtggca cgttcgacat ttccatcatc gagatcgctg
     4921 acgtgcatgg cgagaagcag ttcgaagtgc tgtccacgaa cg
//
LOCUS       DATASH010000997         4039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4325020,
            whole genome shotgun sequence.
ACCESSION   DATASH010000997 DATASH010000000
VERSION     DATASH010000997.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4325020"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /gene="ndhC"
                     /locus_tag="VHV99_26550"
     CDS             complement(<1..206)
                     /gene="ndhC"
                     /locus_tag="VHV99_26550"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX3638979.1"
                     /translation="MNLAAYFPVLLFLIVGTGLGVALVSIGKILGPNKPDTEKNAPYE
                     CGFEAFEDARMKFDVRYYLVAILF"
     gene            complement(368..452)
                     /locus_tag="VHV99_26555"
     tRNA            complement(368..452)
                     /locus_tag="VHV99_26555"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(416..418),aa:Leu,seq:gag)
     gene            complement(565..942)
                     /gene="secG"
                     /locus_tag="VHV99_26560"
     CDS             complement(565..942)
                     /gene="secG"
                     /locus_tag="VHV99_26560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEX3638980.1"
                     /translation="MLYLKTLIIVVQLLSALGVIGLVLLQHGKGADMGAAFGSGASGS
                     LFGATGSANFLSRTTAVLAAVFFVTTLTLTYLGSYRAKPSAGLLGAAVTAPVAASAAA
                     APAGGSAVLPASAASVPATDVPK"
     gene            complement(1025..1804)
                     /gene="tpiA"
                     /locus_tag="VHV99_26565"
     CDS             complement(1025..1804)
                     /gene="tpiA"
                     /locus_tag="VHV99_26565"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX3638981.1"
                     /translation="MSKQRAKLVVGNWKMHGRLAENAALLQAVTQGASELPADVRVGV
                     CVPCPYLAQTQSLLDGSRVVWGVQDVSAFTHGAYTGEVAAQMVVDFGATLAIVGHSER
                     RAYHRESAELVAVKTQRALEAGLTPIVCVGETLEEREAGSTEQVVGMQLDEVLAKLSV
                     EEAGRIVVAYEPVWAIGTGKSATSDQAQAVHAFLRSRLAAKGAAVADVPLLYGGSVKP
                     ENAEELFREPDIDGGLIGGASLKDQDFLAICKAAGATSVAS"
     gene            complement(1937..2953)
                     /locus_tag="VHV99_26570"
     CDS             complement(1937..2953)
                     /locus_tag="VHV99_26570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEX3638982.1"
                     /translation="MKAIEITEFGAPEVLKLAERPMPQPKAGEVLIKVAASGINRPDV
                     FQRKGGYAPPPGASDLPGLEVAGEIVGGGIDEKNNPFGLKVGDRVCALLAGGGYAEYA
                     AAPLLQCLPVPAGLSDVEAASLPETFFTVWSNVFDRAQLGKGEGAPNETLLVQGGSSG
                     IGVTAIQIAHALGFRVFATAGSDEKCRACEALGAERAINYKTEDFVEVIKSLTNDRGV
                     DVILDMVAGSYVPRELTALADGGRIVLIALLGGAKAELNLNEVLRRRLTVTGSTLRPR
                     PIEFKAKIAAQLKERVWPHLEDGRIKPVVHRVFPAAEAAAAHALMESSTHVGKIVLTW
                     GQDA"
     gene            complement(3197..>4039)
                     /locus_tag="VHV99_26575"
     CDS             complement(3197..>4039)
                     /locus_tag="VHV99_26575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX3638983.1"
                     /translation="SEITESNGSSSMASVCGGCLALMDAGVPMKAHVAGIAMGLILED
                     NKFAVLTDILGDEDHLGDMDFKVAGTEQGVTALQMDIKIQGITKEIMQVALAQAKEGR
                     MHILGKMTSAVSGANTQLSEFAPRMITIKINPEKIRDVIGKGGSVIRALTEETGTTID
                     ISDDGIVTIASTSSEGMAEAKKRIENITLEVEVGQVYEGTVLKLLDFGAIVNILPGKD
                     GLLHISEIANERIKDINDYLKDGQQVKVKVIQTDEKGRVRLSANALLNEGANGAPQGE
                     PTPQ"
BASE COUNT          793 a         1357 c         1092 g          797 t
ORIGIN      
        1 atgaagagaa tggcgacgag gtagtagcgc acgtcgaact tcatgcgcgc atcttcgaat
       61 gcttcgaagc cgcactcgta cggtgcgttt ttctcggtat cgggcttgtt gggaccgagg
      121 atcttgccga tgctgaccag tgctacgcct aaaccggtgc ccacaatgag gaacaacaag
      181 acggggaaat aggctgcgag gttcaagact atcctctttc ggttggttct gaatgccgcc
      241 aagagcatag cactcctgac gcgaaatcac ttcgacaacg cacatgccgc aagcccaacg
      301 caagccgcgc gtcagaagtg aaaatgccag ccgaagcgaa gcaagcggct ggcatttaga
      361 taacatttgg tgccgacggc gagactcgaa ctcgcacagc tttcgccact accccctcaa
      421 gatagcgtgt ctaccaattt caccacgtcg gcactaactg caatccggga aaacaactta
      481 taaaacccgc gaatcgcttc aagacttaga ttgtaactgg tctaaacagt ttgttcaacg
      541 cacaaaaaca ctttgaacga aaatttattt cggcacgtcc gtagcgggca cggatgcagc
      601 cgacgccggc aatactgccg agccacctgc cggcgcagct gccgcggaag ccgccacagg
      661 cgcagtcaca gccgcaccca gcaagcctgc ggacggtttc gcacgatagg aaccgaggta
      721 ggtgagcgtc aatgtcgtta caaaaaacac ggctgcgagg acggccgtgg tacgcgacaa
      781 aaagtttgcc gaaccggtcg cgccgaacag gctgcccgat gcgccgctac cgaaagcagc
      841 acccatatcg gcgcctttgc cgtgttgcag caagacaagg ccgatgaccc caagtgccga
      901 caacagctga acgacgataa tcaatgtttt caaatacagc atcacactca cctgagtctt
      961 tatttaaccg cgccacacaa agtgtgccgc gcgggattgg gtcatcctcg ccgaggcgcc
     1021 ctgcttaact ggcgacgctc gtcgcgccgg ccgccttgca gatcgccagg aaatcctggt
     1081 ctttcaacga cgcgccgccg atcagcccac cgtcgatatc cggctcgcgg aacaattctt
     1141 ccgcgttctc cggcttcacg ctgccgccgt acaacaacgg cacatccgct actgccgcgc
     1201 cctttgccgc caggcgcgaa cgcaggaacg catgcacggc ttgcgcctga tccgacgtcg
     1261 cgctcttgcc ggtgccgatc gcccagaccg gctcgtatgc aacgacgatg cgtcccgctt
     1321 cttccaccga cagcttcgcc agcacctcat ccaactgcat gccgaccact tgctccgtcg
     1381 agccggcctc gcgctcctcg agcgtttcgc cgacacatac gatcggggtc aagcctgctt
     1441 ccagcgcgcg ctgcgtcttc accgcaacca actccgcgct ttcacgatgg taggcacggc
     1501 gttccgagtg cccaacgatc gcaagcgtag cgccgaaatc gaccaccatc tgtgcggcca
     1561 cttcgcccgt ataggctcca tgcgtgaacg ccgagacgtc ctgcacgccc cacacgaccc
     1621 ggctgccgtc cagcaacgat tgagtctgcg cgagataagg gcacggcacg cagacgccca
     1681 ctcgcacatc agccggcaat tcactcgcgc cttgcgtcac cgcctgcagc agcgctgcgt
     1741 tttcggcgag gcgcccgtgc atcttccagt taccgactac cagcttggct cgttgtttcg
     1801 acatcgtctc gtcgtctcat cttcttgaat tggcggcgga cttgatttgc gcttacagcc
     1861 cgcctttcgg attcatgcgg cgtgcgcaaa acgcgcaatt ttactgcgtg gggtggatcg
     1921 ggtcaaacag accgtgtcaa gcgtcctgac cccaagtcag cacaatcttg ccgacatgcg
     1981 tgctgctttc catcagcgca tgtgcggcgg cggcctccgc ggctggaaac acacgatgca
     2041 ccaccggctt gatacgaccg tcttcgagat gcggccatac gcgctctttc aattgcgcgg
     2101 cgatcttcgc cttgaactcg atcggccgcg gacgcaacgt ggaacccgtc accgtcaagc
     2161 ggcggcgcag cacttcgttc agattcagct ccgctttcgc gccgcccagc aatgcgatca
     2221 gcacgatgcg gccgccgtcg gccagcgcgg tcaactcacg cggcacataa ctgcccgcca
     2281 ccatgtcgag gatcacgtcg acgccgcgat cgttcgtcag cgacttgatg acttcgacga
     2341 aatcctcagt cttgtagttg atcgcacgct cggcgccgag cgcctcacac gcgcggcact
     2401 tttcgtccga tccggcggtg gcgaacacac gaaagcccag cgcatgcgcg atctggatcg
     2461 cggtcacgcc gataccgctc gagccgccct gcaccagcag cgtctcgttc ggcgcgccct
     2521 cgcccttgcc aagttgcgca cggtcgaaca cattgctcca caccgtgaag aatgtttccg
     2581 gcagcgaagc cgcctcgaca tccgacaagc cggcgggcac cggcaagcac tgcaacagcg
     2641 gcgcggccgc gtactccgcg taaccgccgc ccgcaagcaa ggcacacacg cggtcaccca
     2701 ctttcaaacc gaacggattg ttcttctcgt cgatgccgcc gccgacgatc tcaccggcca
     2761 cttccagccc cggcagatcc gaggcgcccg gcggcggcgc atagccgcct ttgcgctgga
     2821 acacatcagg ccggttgatg cccgaagccg ccaccttgat cagcacctcg cccgctttcg
     2881 gctgcggcat gggccgctct gccaacttca ggacttccgg tgctccgaat tcggtgattt
     2941 cgatcgcttt catctgcgtc aactccaaga ttggattgcg ttgcctaccg gcgccccaca
     3001 gggagtcccc cgccccgaag gaagtcacct tgggggattg ggggacacga atcgcccgac
     3061 tccaacgaaa cgttccatgc aaacagagta cccgcttatg tccgtttgca tcgacctgca
     3121 acgcaatcca ccctgcaccc ttcatgaaaa acggccggcg cgcattcacg ctgccggccg
     3181 tcgtcctgtt accgcattac tgcggcgtcg gttcgccttg cggcgcgccg tttgcgcctt
     3241 cgttcagcaa cgcattggcc gacagacgca cgcggccctt ttcgtccgtc tggatgacct
     3301 tgaccttcac ctgctgaccg tccttcaggt agtcgttgat gtccttgata cgctcgttgg
     3361 cgatttcgga aatgtgcaac agaccatctt tacccggcag gatgttcacg atcgcgccga
     3421 aatcgagcag cttgagcacg gtgccttcgt acacctggcc cacttcgact tccagcgtga
     3481 tgttctcgat acgcttcttc gcttcggcca tgccttcgct gctggtgctg gcgatagtga
     3541 cgatgccgtc gtccgaaata tcgatcgtcg tgccggtttc ttcggtcagc gcgcggatca
     3601 ccgaaccgcc cttgccgatc acgtcgcgga tcttttccgg attgatcttg atggtgatca
     3661 tgcgcggtgc gaattccgac aattgcgtgt tcgcgcccga cacagccgag gtcatcttgc
     3721 cgaggatgtg catacggcct tccttcgctt gcgcgagggc gacctgcatg atttccttgg
     3781 tgatgccctg gatcttgatg tccatctgca gcgcggtcac gccttgctcc gtacccgcca
     3841 ccttgaagtc catgtcgccg aggtgatctt cgtcgccgag gatgtcggtc aggacggcaa
     3901 acttgttgtc ttccaggatc aggcccatcg cgatgccggc gacgtgcgcc ttcatcggca
     3961 cgccggcgtc catcagtgcg aggcagccgc cgcacaccga agccatcgac gatgaaccgt
     4021 tcgattcggt gatttccga
//
LOCUS       DATASH010000998         9694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_566160,
            whole genome shotgun sequence.
ACCESSION   DATASH010000998 DATASH010000000
VERSION     DATASH010000998.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 9694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9694
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566160"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1207
                     /locus_tag="VHV99_26580"
     CDS             <1..1207
                     /locus_tag="VHV99_26580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HEX3638984.1"
                     /translation="KARMNLDARAYHDYKPVAFKAVLESYARLQAAYDTIFVEGAGSP
                     AEINLRERDIANMGFAEAVDCPVVLVADIDRGGVFAHLTGTLACLSESEQARVRGFII
                     NRFRGDVGLLKPGLDWLEAKTGKPVLGVVPYLHGLTLDAEDMLPPELRAAHSGQSDAA
                     GRMLRVVVPVLPHISNHTDFDALRAHPQVDFHYVRSGTPPPAADLIILPGSKNVPGDL
                     AFLRAQGWDAVLQRHLRYGGRVIGICGGMQMLGREVADPHGVEGAPGTSVGLGWFDYS
                     TVLTREKTLKNVTGRLALAGSPQVAGYEIHMGETQGPALNAPALQLGEAPDAHPDGAI
                     SADGQILATYVHGLFDTPAACAALLAWAGLSDAGEIDYPALREASLDRLADTLAEHLD
                     LSKLFAAIG"
     gene            complement(1400..1819)
                     /locus_tag="VHV99_26585"
     CDS             complement(1400..1819)
                     /locus_tag="VHV99_26585"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX3638985.1"
                     /translation="MSELRQEYTIERLHERQKGTLPDLLGVHILALEHGTLSAELIVR
                     EELLAPNGFLHAATVIGLADTACGYACLAHLPETARNFTTVELKSNFLGTATEGTIRA
                     VAKAVHLGRSTQVWDATVTDPNGRTIALFRCTQMVLY"
     gene            2029..2667
                     /locus_tag="VHV99_26590"
     CDS             2029..2667
                     /locus_tag="VHV99_26590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015001984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX3638986.1"
                     /translation="MTVIVVANPKGGVGKSTLSTNLAGYFASLGEWVALADLDKQHSA
                     QAWLSLRPPALPPIETWEVDPENPVKPPRGLEHAVVDTPAGLHGNRLNIALDLADKVI
                     VPLQPSMFDILATQEFLERLAKEKAVRKGAIEIGVVGMRVDARTRSAEQLHRFVEGLK
                     LPVLGYLRDTQNYVQLAAHGLTLWDVAKSRVEKDLEQWQPIIQWTDGAGKKA"
     gene            complement(2847..3233)
                     /gene="panD"
                     /locus_tag="VHV99_26595"
     CDS             complement(2847..3233)
                     /gene="panD"
                     /locus_tag="VHV99_26595"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEX3638987.1"
                     /translation="MQRNMLKSKIHRVAVTHCELHYEGSCAIDEDLLEAANIVENERI
                     DIWNINNGERFSTYAIKGERGSGMISLNGSAARRAQLGDLVIIAAFAVVDETELKAGW
                     KPDLVFVDDNNKIKGSRDHVPTQNWT"
     gene            complement(3284..4120)
                     /gene="panC"
                     /locus_tag="VHV99_26600"
     CDS             complement(3284..4120)
                     /gene="panC"
                     /locus_tag="VHV99_26600"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011352891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX3638988.1"
                     /translation="MKVISSIHELRDQLRGQNRTAFVPTMGNLHEGHLSLMRLARQHG
                     DPVVASIFVNRLQFGPNEDFDKYPRTLEADIDKLQKEGVYVLFAPTERDLYPEPQEYR
                     VHPPHDLGDILEGEFRPGFFQGVCTVVMKLMSCVQPRVAVFGKKDYQQLMIVRSMCHQ
                     FALPTDIIAAETVRDADGLALSSRNRFLQTAERAEAPVLAAELNRVRDAVLAGDRDFA
                     KLEQAAMAALAARGWQPDYIAVRKRSNLLPPASPQDANAELVVLAAAKLGTTRLIDNL
                     EI"
     gene            complement(4341..5213)
                     /locus_tag="VHV99_26605"
     CDS             complement(4341..5213)
                     /locus_tag="VHV99_26605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010102217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HEX3638989.1"
                     /translation="MSHADEAKGATAAPDAALAAPATDSTPDIVDGIAFARLYGEPLF
                     KLPTDLYIPPDALEVFLETFEGPLDLLLYLIRKQNFNVLDIPMADVTVQYLGYVDQLR
                     QTNLELASEYLLMAAMLIEIKSRMLLPMKKADSGDEAEDPRAELVRRLLEYEQMKLAA
                     QRIDHLPQLGRDFLRAEVYIEQSITPRFPDVDSEDLRAAWADVIKRAKLVQHHKISRE
                     ELSVREHMSTILRRLQNARFMEFSDLFDTSKGVPVVVVNFIAMLELCRESLIEITQAE
                     PFAPIYVRLAYLPA"
     gene            complement(5218..5403)
                     /locus_tag="VHV99_26610"
     CDS             complement(5218..5403)
                     /locus_tag="VHV99_26610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008924036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HEX3638990.1"
                     /translation="MYQSDITQFLNQLKQQKPNLEAEQRRGRALLWDKQPIDLEERAE
                     QKASRVEQTPYSYYQNF"
     gene            5811..7649
                     /locus_tag="VHV99_26615"
     CDS             5811..7649
                     /locus_tag="VHV99_26615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex family protein"
                     /protein_id="HEX3638991.1"
                     /translation="MAGCAIEEPRMRRGKSGDARGHLVPIRRWLRAWLTFGIVMLMLA
                     TVDAHAARRAAASYKVDIEATPRSLRKLLEAHLDVARFARRPDISDDQFEFLITATSQ
                     QVRDLAATQGYFTPVVRTDVRTVDDQKHVTVSVDPGPQTLISSISLSFRGPVLTEDPA
                     QENAARFAFSLHEGDPFSQGDWDDAKNASLKALQARRYLGAKIYHSEARIDPRTHEAK
                     LSVTYESGPTFTMGKLDVSGTRRYPEQIVDNVNPISVGDIYDVQRVTELQRQLQNTPY
                     YASVAIDVDSDPAKPVDTPMHVKVSEYPYNSIRGGVGYSTDNGPLVQGSYSYLDTFGK
                     AWPFTISGRVDQIQQYGQIQLAMPPGPRAWTNSVLASYTTTDVSDTRIYSIRGGVQRA
                     RTSQFIDYNYSILYYQDRLDQNAIAPTTSRALVPSWSWTRRNTDDPLFPRSGNLIHVE
                     TGFAVKGVLTDQTFVRGYARGQQYLPIGKEDLVLFRAELGGVFTSGSSSGIPASLLFR
                     AGGSNSIRGYSYESIGNNVDGSVLPTKYLVTASAEYQHWFTHDWGAAAFFDVGTATDT
                     WGEKVFYPGVGVGARWRSPVGPVNVDVAYGIRNRSVRPYLTLGIAF"
     gene            7678..>9694
                     /locus_tag="VHV99_26620"
     CDS             7678..>9694
                     /locus_tag="VHV99_26620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation and assembly module protein TamB"
                     /protein_id="HEX3638992.1"
                     /translation="MTTDVSAQPPAQPPGAHPPGQPPGQQPAPDAPQPKRRRARLLLK
                     TLVWTLAMLVLLVAVAIGLLYGALTTERGTAYAWQAAVKLLGGKLTGTLDSGALSTGV
                     QLRQVRWRSLDGSGTDIQIDRVAGRWALSRKPWRFAIDYLHIGTIDARVGPSSSSSSG
                     PLKLPQDLRLPMQLEVRDVEVDKLLLRQGGSTTELSRFMFHGRSDGRHHEAAIDRLDT
                     PFGAVTVAAKLDGVRPFPLTGDVGYSGKVNNEAVQVGGHLSGSLENLIAELDASGMKL
                     AGHARVEATPFGDVPLQRATLTFDHVNPQAFAPGAPLADLAVRAELAPVGQGAAGAVV
                     LGAASGASGVSGAGAAGAASPAGASGTASAGRAGGTAKSAGNASAGSTRSASNASAGS
                     TGNATSAAPNNAPGIAGRHVVKNGKGGVNTDATEKGAAGFAVAGHVSIANAKPGAIDQ
                     NLLPLIDAQTDVRLDAQAQRISNLNVRLVKSATLTGGGALTGKRGQFDLHVAGLDLNA
                     LEATVRPTQLSGPIGIRLNDDVQSLTLDLADPKAGLRAQGKVTFDPARMSFNDVRITS
                     GKGRIDLSGALKHDANSTYNLKAQLTDFDPLTLASQMPSRTPVTTAAGTAKATQATKA
                     TKAKATQATQATQATQANQANQANQANQANQANQANQANQANQANQAN"
BASE COUNT         1769 a         3027 c         3152 g         1746 t
ORIGIN      
        1 caaggcgcgc atgaatctcg atgcacgcgc gtatcacgac tacaagccgg tcgccttcaa
       61 ggctgtgctg gaatcgtatg cgcgtctcca ggcggcgtac gacacgatct tcgtcgaagg
      121 cgcgggcagc cccgctgaga tcaatctgcg tgagcgcgat atcgccaaca tgggtttcgc
      181 tgaagcggtc gattgtccgg ttgtcctggt ggccgacatc gaccgcggtg gggtgttcgc
      241 gcatctgacc ggtacgctcg cgtgtttgtc ggaaagcgag caggcgcggg tgcgcggctt
      301 catcatcaac cgctttcgcg gcgatgtcgg tttgctcaag cccgggctcg actggctcga
      361 agcgaagacc ggcaagccgg tgctcggcgt ggtgccctac ctgcacggtc tgacgctcga
      421 cgccgaagac atgctgccgc ccgaactgcg cgccgcgcat agcggccaaa gcgacgcggc
      481 gggccggatg ttgcgagtgg tcgtgccggt gttgccgcac atcagcaacc acaccgattt
      541 cgatgcgctg cgcgcgcatc cgcaggtcga ttttcattac gtgcgaagcg gcacgccgcc
      601 tccggcagcc gatctgatca ttttgccggg ctcgaagaat gtgcccggcg acctggcctt
      661 tctgcgtgcg cagggctggg atgcggtgct gcaacggcat ctgcgctacg gcggccgtgt
      721 gatcgggatt tgtggcggca tgcagatgct ggggcgcgaa gtggccgatc cgcacggcgt
      781 cgaaggcgcg cctggaacga gcgtgggcct cggctggttc gattattcga ccgtgttgac
      841 gcgtgaaaag acgctgaaga acgtgacagg gcgccttgca ttggcgggtt cgccccaggt
      901 cgccggctac gagattcaca tgggagagac gcaaggcccg gcgctgaatg cccccgcgct
      961 gcaattgggc gaagcgccag acgctcatcc cgacggcgca atctcggccg atggccaaat
     1021 cctcgctacc tacgtgcacg gcctgttcga tacgccggcc gcgtgcgccg cattgctcgc
     1081 gtgggcgggg ttgagcgatg ccggggaaat cgactatccg gcgttgcgtg aggcttcgct
     1141 ggatcgtttg gccgacacgc tcgccgaaca tctcgatctg tcgaagcttt ttgccgcgat
     1201 tgggtgacgg ttcgatcgga tggtcgtgat tttcccgtat tgggaaacaa tcaagataaa
     1261 aaacgcagtt ttttgcctcg ttgcaccttg ccggacaacg aaaaaagcac gcgctgtgat
     1321 gccgcgtgct tttttttttg gcgtcgcgcc cagataattt gggcacttcg agccccttcg
     1381 ccacgcccgc atcactcagt caatacagca ccatctgcgt gcagcgaaac agcgcgatcg
     1441 tcctgccgtt cggatcggtg acggtggcgt cccacacctg cgtactgcgg cccagatgca
     1501 cggccttggc gaccgcgcgg atcgtgccct ccgttgccgt accgagaaaa ttgcttttca
     1561 gctccacggt ggtgaaattg cgcgccgtct cgggcaagtg ggccaggcat gcatagccgc
     1621 aggcggtatc ggcgagaccg atcacggtgg cggcatgcag aaagccgttc ggtgccagca
     1681 attcctcgcg cacgatcaat tccgcgctca gagtgccgtg ctccagcgcc agaatatgca
     1741 cgcccagcaa atcgggcagc gtgcccttct ggcgctcatg caggcgctcg atggtgtatt
     1801 cctggcggag ttcgctcatg agattcgagt cctcgatgga aaaaacaggg ggtagggcgg
     1861 ccaattagcc gtttgggggc ttcggccccg gtgcatttgt cgatattatc aacggctgga
     1921 tcggcgggtg aacctgaaac ggcacgaatc aggacacgcc cgtgcaagtc ggagcaaaca
     1981 gcgcggcaaa ccggctggcg gcgcccagcc ttctttccgg gagaattcat gacggtgatc
     2041 gtggtggcga atccgaaggg cggcgtgggc aaaagcacgc tgtccaccaa tctggcgggc
     2101 tatttcgcgt cgctcgggga atgggtcgcg ctggcggacc tcgacaaaca gcactccgcg
     2161 caagcgtggc tgtcgctacg gcccccagcg ctaccgccca tcgagacttg ggaggtcgat
     2221 ccggaaaacc ccgtcaaacc acccaggggt ctggaacacg cggtagtgga cacgccggcc
     2281 gggttgcatg gcaaccgtct gaacatcgcg ctggatctgg ccgacaaggt gatcgtgcca
     2341 ctgcagcctt ccatgttcga tattctcgcc acccaggaat ttctcgagcg gctggcaaaa
     2401 gagaaggcgg tcaggaaagg cgcgatcgag atcggcgtcg tggggatgcg cgtcgacgcg
     2461 cgcacgcgct cagcggagca gttgcatcgt tttgtcgagg gattgaagct gccggtgctg
     2521 ggctatctgc gcgacacgca gaattacgtg caactcgcag cgcacggcct gacgctgtgg
     2581 gacgtcgcaa aaagccgcgt ggagaaggat ctggagcagt ggcaaccgat catccaatgg
     2641 actgacggtg ccggcaagaa ggcttgaggg agcttgggcg gctcgaggcg accgacgtcg
     2701 gctcagagtg agcctgagac gcgcttgaag caggcttgat gagaagtctt gcgtgcccgc
     2761 ggggagccct tgaggcgtgc ctgaaatgcc gtccagatac gcggcgtggc agcccgccgc
     2821 ttaaaaccgc gccgtgaacg acaggttcag gtccagttct gggtcggcac gtgatcgcgg
     2881 ctgcccttga tcttgttgtt gtcgtcgacg aacaccagat ccggcttcca gcctgccttc
     2941 agctccgttt cgtcgaccac cgcgaacgcc gcgatgatca ccagatcgcc cagttgtgcg
     3001 cgccgcgcgg ccgaaccgtt cagcgaaatc atgccgctgc cgcgttcgcc cttgatcgca
     3061 tacgtcgaga aacgctcgcc gttgttgatg ttccagatgt cgatccgctc gttttcaacg
     3121 atgttcgcgg cttccagcaa gtcttcgtcg atcgcgcacg agccttcgta gtgcagttcg
     3181 cagtgcgtga ccgcgacgcg atggattttc gacttcagca tgttgcgttg catgaccaat
     3241 cccttgtggc ttcttacgcg cgcttgcgca gactaagacg gcttcagatt tcgaggttgt
     3301 cgatgagccg agtggtgccg agtttggcag cggccagcac gaccaactcc gcgttcgcgt
     3361 cttgcgggct cgccggcggc agcagattcg agcgcttgcg cacggcgatg tagtcaggct
     3421 gccagccgcg cgcggcgagt gccgccattg ccgcctgttc gagtttcgcg aagtcgcgat
     3481 cgccggcgag gaccgcatcg cgcacgcgat tgagttcggc ggccagcacc ggcgcctcag
     3541 cgcgctcggc cgtctgcaga aagcggttac gcgaactgag cgcgaggccg tcggcgtcac
     3601 gcacggtttc cgccgcgatg atgtcggttg gcagcgcgaa ctggtggcac atgctgcgca
     3661 cgatcatcaa ttgctgataa tcctttttgc cgaacaccgc gacgcgcggc tgcacgcacg
     3721 acatcagctt catcaccacc gtgcacacgc cctggaagaa gcccggccgg aactcgcctt
     3781 cgaggatgtc gccgagatcg tgcggcggat gcacacggta ctcctgcggc tccggataca
     3841 gatcgcgctc cgtcggcgcg aacagcacgt agacgccttc tttctgcagc ttgtcgatgt
     3901 cggcttcgag cgtgcgcggg tatttgtcga aatcttcgtt aggaccgaac tgcaagcggt
     3961 tgacgaagat gctcgccacc accggatcgc cgtgctggcg cgcgaggcgc atcagcgaca
     4021 gatggccctc atgcagattg cccatggtcg gcacaaaggc ggtgcgattc tggccgcgca
     4081 actggtcgcg caattcatgg atcgagctga tgactttcat gatcggagag gaatttcctc
     4141 gcgcggcgcg agactcggtg ggcgcaaaaa ctcaggtttt cgcacgtccc gcagcctcgc
     4201 tggtgatgac aggttcggag cgcgggattg tagtggaatt gttccggcgg cgcaccaagg
     4261 tagtggattt tgttccgtgg agcgcactga aacgaggcgt ccgcggcagc aatggcgcac
     4321 gcagaccccg ttttgcggcc tcaggccggc agataggcga ggcgcacata gatcggggcg
     4381 aacggctcag cctgagtgat ttcgatgagc gactcacggc acagctcgag catggcgatg
     4441 aaattcacca cgaccacggg cacgcccttg ctcgtgtcga acagatcgga gaattccatg
     4501 aaacgcgcgt tctgcaagcg ccgcaggatc gtgctcatat gctcgcgcac cgacaattcc
     4561 tcgcgcgaaa tcttgtggtg ctgaaccagc ttggcgcgct tgatcacgtc ggcccacgcg
     4621 gcgcgcaggt cttcgctatc cacatccgga aagcgcggcg tgatgctttg ctcgatatac
     4681 acctcggcgc gcaggaaatc gcggccaagt tgcggcagat ggtcgatgcg ctgtgcggcg
     4741 agcttcatct gctcgtattc gagcaggcgg cgtaccagtt ccgcacgcgg atcttcggct
     4801 tcgtcgccgc tatcggcctt cttcatcggc aacagcatgc gcgacttgat ctcgatcagc
     4861 atcgcggcca tcagcagata ctcggacgcg agttcgagat tggtctgacg caactggtcg
     4921 acgtagccga ggtattggac cgtgacatcc gccatcggga tgtcgagcac attgaaattc
     4981 tgcttgcgga tcaggtacag caacagatcc agcgggcctt cgaaggtttc gagaaacacc
     5041 tcgagcgcgt ccggcgggat gtacagatcc gtcggcagct tgaagagcgg ctcgccgtac
     5101 aggcgcgcga acgcaatgcc gtcgacgatg tccggcgttg aatcggtggc gggcgcggca
     5161 agcgcggcgt caggcgccgc tgtggcgccc tttgcctcgt cggcgtgact cacgcgctta
     5221 gaagttttga tagtacgagt aaggcgtctg ctctacgcgc gaggctttct gctcggcgcg
     5281 ttcttcgagg tcgatcggct gtttgtccca cagcagcgcc cggccacggc gctgttcagc
     5341 ttccagattc ggtttttgtt gcttgagctg gttcaggaac tgggtgatgt ccgattgata
     5401 catggctaga cgtccgtggt ttggggaatg gcggctgccc agcgggaatc ccgcgcggcg
     5461 tcgctgctat tccataattt taccgcatgc gccgcgcgcg gtcgaaaaac gctgaccgac
     5521 gcgagcggcc agcctcccaa aacgtccgga acccgcgctg ctgcgatgcg tcaaaggcgg
     5581 gcgcgtcgct ggcggacggt gtgcgaatcc gggatgtgcg tccgggcgga tggcgttgcg
     5641 caggctattt cagcgcggcg taccagccaa ggggcgcgcc gctcccgacg cggcaaacgc
     5701 gcctttttaa gacgtggcgc ggcttcgccg atgtggtcaa atagcgcgag cttcggccgc
     5761 cgcggcgcgc cagagcgaca gaattcatca accacggccg gaggtcctgc ttggcggggt
     5821 gtgcgatcga agagccgcgt atgcggcgcg gcaaaagcgg tgacgcgcgc gggcatctgg
     5881 tgcccattcg gcgctggctg cgcgcgtggc tcacgttcgg catcgtcatg ctcatgctgg
     5941 ccacggtcga cgcgcacgcg gcgcggcgcg ccgccgccag ctacaaggtc gatatcgaag
     6001 ccacgccgcg ctcattacgc aagctgcttg aagcgcacct ggacgtggcg cggttcgcca
     6061 ggcgtcccga catcagcgac gaccagttcg aattcctgat taccgccacg tcccagcagg
     6121 tgcgcgatct ggccgccacg cagggctatt tcacgccggt cgtgcggacc gacgtgcgta
     6181 ccgtcgacga ccagaagcac gtcacggtga gcgtcgatcc cggcccgcag accctcattt
     6241 cgtcgatttc gctgtcgttt cgcggcccgg tgctgaccga agacccggcg caggaaaacg
     6301 ccgcgcgctt cgcgttttcg ctgcatgaag gcgatccgtt ttcgcaaggc gactgggacg
     6361 acgcgaaaaa cgcgtcactc aaggccttgc aggcgcgccg ttatctgggc gcgaagattt
     6421 accactccga ggcgcggatc gatccgcgca cgcacgaagc gaagttgtcg gtgacgtatg
     6481 aaagcggtcc gaccttcacg atgggcaagc tcgacgtatc cggcacgcgg cgctatccgg
     6541 aacagatcgt cgacaacgtg aatccgattt cggtcggcga tatctacgac gtgcagcgcg
     6601 tcaccgaatt gcagcggcag ttgcagaaca cgccgtacta cgcgagcgtg gcgatcgacg
     6661 tggatagcga tccggccaag ccggtcgaca cgccgatgca cgtgaaggtg tcggaatatc
     6721 cgtacaacag tatccgcggc ggggtcggtt actcgaccga caacggcccg ttggtccagg
     6781 gttcgtactc gtatctcgac acgttcggca aggcgtggcc gttcacgatc agcggacgcg
     6841 tcgaccagat tcagcagtac ggacagattc aactcgcaat gccgccgggg ccgcgcgcat
     6901 ggaccaatag cgtgctggct tcctacacca cgaccgacgt ctccgacacc cgcatctaca
     6961 gtattcgcgg cggcgtgcag cgggcccgta cgtcgcaatt catcgactat aactactcga
     7021 tcctttacta ccaggaccgg ctcgaccaga acgcgatcgc gccgaccacc agccgcgccc
     7081 tggtgccgtc atggtcgtgg acccgccgca ataccgacga cccgctgttt ccgcgttcgg
     7141 gcaacctgat tcacgtcgaa actggcttcg cggtcaaagg cgtgctgacc gatcagacgt
     7201 tcgtgcgcgg ctacgctcgc ggccaacagt acctgccgat cggcaaggaa gacctcgtgc
     7261 tgttccgtgc tgaactcggc ggcgtgttca cgagcggcag ttcgagcggg attccggctt
     7321 cgctgctgtt ccgcgcgggt ggttcgaatt cgatccgtgg ctacagctat gagagcatcg
     7381 gtaacaacgt cgacgggtcg gtgctgccta ccaagtatct cgtgacggcg tcggccgaat
     7441 atcagcactg gttcacgcac gactggggcg cagccgcgtt cttcgacgtc ggtaccgcca
     7501 ccgatacctg gggcgagaaa gtcttttatc ccggcgtcgg tgtcggagcg cgttggcgca
     7561 gcccggttgg cccggtcaat gtcgacgtcg cttacggcat ccgcaacagg agcgtgcggc
     7621 cgtacctgac gctcggcatc gccttctgat ggaaacctgc tcttacatga tccacgcatg
     7681 accacggacg tttccgccca accacccgcg cagccgcccg gcgctcatcc gcctggacag
     7741 ccgcccgggc agcagcctgc gcctgacgcg ccgcagccga agcgccgtcg cgcccgcctg
     7801 ctgctgaaaa cgctcgtgtg gacgctcgcc atgctggtgc tgctggttgc cgtggcaatc
     7861 ggcctgctgt acggcgcgct gacgaccgag cgcgggactg cctacgcgtg gcaggcggcc
     7921 gtcaaactgc tcggtggcaa actgaccggc actctggaca gcggtgcgct ttcgaccggc
     7981 gtgcaactgc ggcaggtgcg ctggcgcagt ctcgacggca gcggcaccga tatccagatc
     8041 gaccgcgtgg ccggccgctg ggcactttcg cgcaagccct ggcgcttcgc gatcgattat
     8101 ctgcatatcg gtacgatcga cgcccgtgtc ggcccttcgt cgtcgagcag cagcgggccg
     8161 ctgaaactgc cgcaagacct gcgcttgccg atgcaactcg aagtgcgcga cgtagaggtc
     8221 gacaaactgc tgttgcgcca gggcgggtcg acgaccgagc tttcccgttt catgttccac
     8281 ggccgcagtg acggccggca ccacgaagcc gcgatcgatc gattggacac gccgttcggc
     8341 gcggtgacgg tggcggccaa gctcgacggg gtgcggccgt ttccgctgac cggcgacgtc
     8401 ggctattccg gcaaggtcaa caacgaggcg gtgcaggtgg gcggccatct gagcggctcg
     8461 ctggagaatc tcatcgccga actcgatgcc agcggcatga agctcgccgg ccatgcgcgg
     8521 gtcgaggcca cgccattcgg cgacgtgccg ttgcaacgcg ccacgctgac tttcgatcac
     8581 gtcaatccgc aggcatttgc gccgggtgcg ccgctggcgg atctggcggt gcgggcggaa
     8641 cttgcgccgg tggggcaggg tgcggcaggg gcggtggtat tgggcgcggc tagtggggcg
     8701 agcggggtga gtggtgctgg cgcggcaggg gcggcgagtc ccgccggcgc ttccggcacc
     8761 gcgagtgccg gccgtgctgg cggcaccgcc aagagtgccg gtaacgcgag tgctggcagc
     8821 accagaagtg ccagtaatgc gagtgccggc agcaccggta atgccactag cgctgctccg
     8881 aacaacgctc ccggcattgc cgggcggcac gttgtcaaaa acggcaaagg cggcgtcaat
     8941 accgacgcga cagaaaaagg cgcagcgggt ttcgccgtcg ccggccatgt ctcgatcgcc
     9001 aacgccaaac ccggtgcgat cgaccagaat ctgctgcctt tgatcgacgc gcaaaccgac
     9061 gtgcgcctcg acgcccaggc gcagcgcatc tcgaacctga acgtacggct ggtgaaaagc
     9121 gccacgctca ccggcggcgg cgcgctgacc ggcaagcgcg gccagttcga cctgcacgtg
     9181 gccgggctcg acctgaacgc gctcgaagcg accgtgcggc cgacccagct ttccggtccg
     9241 atcggcatcc gtctgaacga cgacgtgcaa agcctgaccc tcgatctcgc cgacccgaaa
     9301 gcggggctgc gcgcccaggg caaggtgacc ttcgacccgg cgcgaatgag cttcaacgac
     9361 gtgcggatca cgtcgggcaa aggccgcatc gatctgtccg gcgcgctcaa gcacgacgcg
     9421 aattcgacct acaacctgaa agcgcaactg accgatttcg atccgttgac gctggcttcg
     9481 cagatgccgt cccgcacgcc ggtaaccacg gcggcaggga ctgccaaggc gacccaggcc
     9541 acgaaggcca cgaaggccaa ggccacccag gcgacccagg cgacccaggc gacccaggca
     9601 aaccaggcaa accaggcaaa ccaggcaaac caggcaaacc aggcaaacca ggcaaaccag
     9661 gcaaaccagg caaaccaggc aaaccaggca aacc
//
LOCUS       DATASH010000999         1605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1359539,
            whole genome shotgun sequence.
ACCESSION   DATASH010000999 DATASH010000000
VERSION     DATASH010000999.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1359539"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1419)
                     /gene="pnp"
                     /locus_tag="VHV99_26625"
     CDS             complement(<1..1419)
                     /gene="pnp"
                     /locus_tag="VHV99_26625"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX3638993.1"
                     /translation="MRMFKKIVKEFKWGQHNVRLETGEIARQAGGAVIVDVEDTVVLA
                     TVVGAKTAKPGQDFFPLTVDYLEKTYAAGKIPGGFFRREGRPSEGETLISRLIDRPLR
                     PLFPEGFYNEVQVVIHVLSLNPDVPADIPALIGASAALAISGLPFNGPVGAASVAYIN
                     NEFVLNQTRPQMKESALDLIVAGTERAVLMVESEAQQLTEEVMLAAVVFGHDQMQIAI
                     DAIHELVAEGGKPEWDWVPAPKNEPLIARVTELAQAELLSVYQIRDKSARSAKLKEVY
                     ASTTAKLTEEAVGKGETPADKATIGNVMFDIEAKIVRTQILNGEPRIDGRDTRTVRPI
                     EIRTGVLPRTHGSALFTRGETQALVIATLGTKGDEQNIDALEGEYRERFMLHYNMPPF
                     ATGETGRVGSPKRREIGHGRLAKRALAACLPSADEFGYSIRVVSEITESNGSSSMASV
                     CGGCLALMDAGVPMKAHVAGIAM"
BASE COUNT          296 a          513 c          477 g          319 t
ORIGIN      
        1 catcgcgatg ccggcgacgt gcgccttcat cggcacgccg gcgtccatca gcgcgaggca
       61 gccgccgcac accgaagcca tcgacgacga accgttcgat tcggtgattt ccgacacgac
      121 acggatcgag tagccgaatt cgtcggcgct cggcaggcaa gcagccagcg cgcgcttggc
      181 cagacggccg tggccgattt cacggcgctt cggcgaaccg acgcggcccg tttcgccggt
      241 cgcgaacgga ggcatgttgt agtggagcat gaagcgctcg cgatactcgc cttcgagcgc
      301 gtcgatgttc tgctcgtcgc ccttcgtacc gagcgttgcg atcaccagcg cctgcgtttc
      361 gccgcgcgtg aacagcgccg aaccgtgcgt acgcggcaac acgccggtac ggatttcgat
      421 cggacggacg gtacgcgtgt cgcggccgtc aatacgcggc tcgccgttca ggatctgcgt
      481 acggacgatc ttcgcttcga tatcgaacat cacgttgccg atagtcgcct tgtcagccgg
      541 cgtttcgccc ttgccaacgg cttcttcggt cagcttggcg gtcgtcgatg cgtagacttc
      601 cttcagcttg gcgctgcgag ccgacttgtc gcggatctga taaacggaca gcaactcggc
      661 ttgcgccagt tccgtcacgc gtgcgatcag cggttcgttc ttcggcgccg gcacccagtc
      721 ccactcaggc ttgccgcctt cagcgacgag ttcatggatt gcatcgatag cgatttgcat
      781 ctggtcgtga ccgaacacca cggcggcgag catgacttct tcggtcagct gctgcgcttc
      841 cgattccacc atcagcactg cgcgttccgt gccggccacg atcaaatcga gcgccgattc
      901 cttcatctgc ggacgcgttt ggttcagcac gaattcattg ttgatatacg cgacgctggc
      961 agcgcccacc gggccgttga acggcagccc ggagatggcc agagccgccg acgcgccgat
     1021 cagcgcgggg atgtcggcag gcacatcggg attgagcgac agcacgtgaa tcacgacctg
     1081 gacttcgtta tagaagcctt ccgggaacag cggacgcaac gggcggtcga tcaggcgcga
     1141 gatcagcgtt tcgccttccg acggacggcc ttcgcgacgg aagaaaccac ccggaatctt
     1201 gccggctgcg taggtctttt ccagataatc gacggtcaac gggaagaagt cctggcccgg
     1261 ctttgccgtt ttcgcgccga caaccgtcgc gagcaccacg gtatcttcca catcgacgat
     1321 cacggcgcca ccggcctgac gtgcgatttc accggtttcc aggcgaacgt tgtgttgtcc
     1381 ccacttgaat tccttgacga tttttttaaa cattctcact ccttcttcgt tatgcctcgc
     1441 agcgtcctgg cgtgttgtcg tgtgctccgg agcatccgga acccgcgcac acgccgatga
     1501 aagctgcgga gccaagcgat aaccggaacc gcgcaagaga ggggtatatg tcattccaac
     1561 gcaacacgac acgcgatgcg catgacgcgc tggaatgaca caaag
//
LOCUS       DATASH010001000         6405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4722704,
            whole genome shotgun sequence.
ACCESSION   DATASH010001000 DATASH010000000
VERSION     DATASH010001000.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6405
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4722704"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VHV99_26630"
     CDS             complement(<1..207)
                     /locus_tag="VHV99_26630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018902755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HEX3638994.1"
                     /translation="MSEFCNESGGRQSLDELDTPCLLLDEARMTRNIERLRAHLGTLG
                     VQLRPHLKTPKSIEVAQRTMDSAAG"
     gene            complement(204..1760)
                     /locus_tag="VHV99_26635"
     CDS             complement(204..1760)
                     /locus_tag="VHV99_26635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HEX3638995.1"
                     /translation="MKSPRIAILGFAIESNRFAPVSTRADFVSRAYLQDAALLADAKS
                     AAPVMTPEIPAFVRTMDAIRPWTPVPILFANAESGGPVEHGFFADTLREFEQRLRAAL
                     PLDAVYICEHGAAITTEEDDPDGAVFTLVRSIVGPAVPIVATVDLHANISDRMVDAVD
                     ALVSYRRNPHTDMAERGADAARIMIELLDGMRTSVAHVRMPVCSPPTQLLTAPGTGPY
                     AEMIRRAEALDDPRIVNISVAGGFAFADTPKNGLTVLVTTRGDAQLAAQIAHDLASYS
                     WQERAAFTPQLTSLDEAVRVAKATGDDASRTPVLLADVADNPGGGGRGNTPFILKALH
                     EQDVRGAILGVITDPDLVADAYEAGIGETFHARFNRSETTVYSDPFDVPARVVRLHDG
                     KGVGRRGQLAGCSFDLGPSVALQIGGVTVIVISKRHQCHEPMFFEMFGIDIASARVVA
                     LKSRGHFRAAFDEFFSGEQILSVDAPGLTSPILARFDFKKLPRPVVPMDDIANWIPTV
                     RAVQPRSDAQ"
     gene            1975..2874
                     /locus_tag="VHV99_26640"
     CDS             1975..2874
                     /locus_tag="VHV99_26640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3638996.1"
                     /translation="MISTEDLHFFITVTASASLADAARKLDVTPPAVTQRLRSLEKRL
                     GMRLIDRSGRHLVLTDEGELFALHARRICDDIGNLSDTLASRRGTVAGHLRVIAPLGF
                     GQRYVAPAVAQFRCLYPEVTASLMLSDRPAHISDDMWDLMVHIGELRDSTLVSRRLVP
                     NRRVLCAAPQYLAQRGEPRHPDDLRVHDCIALRENDEDVTMWRFMPKHHGTPANVRIE
                     PFLTSNDGGTVRDWALAGLGIIVRSEWDVAEHVRAGRLVPLLDEWQLPDADIVALISS
                     RHGRSLRATRFLDHLHSSLSQLR"
     gene            complement(3023..3994)
                     /locus_tag="VHV99_26645"
     CDS             complement(3023..3994)
                     /locus_tag="VHV99_26645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012733392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX3638997.1"
                     /translation="MPSDAQLLLLDKNEVESLLLPDDVMQAVREAFVLHSQREGRVFR
                     VVREPLVTGGIFGIKSGDVPAQGLLGFKAAGFWPTNRALNGEPHQATIMLIDPATGRP
                     LCMIDGNAVTTMRTGAAGGLGLLHLARADSTRVCVFGSGVQARVQLTFALQLMPSLVR
                     VRYLTANGQRDPAFEAHFEQRCDISHANDGDRAVSESDIVITATPGAGALFDLHSVQP
                     GTHLNCVGADTKGKRELPEGLLGRVRLFVDDQVQARQIGETQWAADHPCTELGDLLTG
                     KAQANRREDDITVFDMTGLALQDLTVARMLCERARETGVGTSLAWPW"
     gene            complement(3984..4613)
                     /locus_tag="VHV99_26650"
     CDS             complement(3984..4613)
                     /locus_tag="VHV99_26650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005014755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX3638998.1"
                     /translation="MSNQTKEQQVLVAQLKQVALGLGETFAPFCEVVVHDLLDAEHAI
                     AAIHNNLSGREVGDPATELGLARIGDAAYPQVVANYANRFADGRQAKSTSIGIKDSKG
                     QYVAALCINIDLTLFGALQNVLGQFARTDDSAGTNEKLDPPGAEGIRMRIDQFAARLA
                     TTPRALKTEERRILMKELRESGSMDVRRSMEVVAQHLGVSRATVYNDAK"
     gene            complement(4942..5850)
                     /locus_tag="VHV99_26655"
     CDS             complement(4942..5850)
                     /locus_tag="VHV99_26655"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3638999.1"
                     /translation="MLNSAYRYFYAVALEGSIRRAAERVHISASALSRQVRLLESEFG
                     QALIEQHGRRIVLTGAGNILREHIRQLMLQDAAVKDAIRTSNGLQFGHVKVASGNGYV
                     PDLADAVIPEFLARHPQMTYGIDIGNGDAVLRMVIDEVVDIGITMNPPQHHLIKVVHS
                     APAPLLVVLPLGHALSSAESVSLDDLQQFAIGLLPSQHRIRQSIKVVEDAVGIKLQPV
                     IESNSYEILKSLVKNKLAITILPEFAIKTSGWESIFKLIPLQHERLAQTTSGIIARQG
                     RAIPESVMEFVSFVIARHNAFSGSLP"
     gene            complement(6184..>6405)
                     /locus_tag="VHV99_26660"
     CDS             complement(6184..>6405)
                     /locus_tag="VHV99_26660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639000.1"
                     /translation="KRTGQGELLDATVLIFERYRVSQGSDAARVAGFSGGGLLSTGLA
                     RTPEAHSVCSKPVGCVATVSVRFGGASRP"
BASE COUNT         1296 a         1944 c         1835 g         1330 t
ORIGIN      
        1 acccgctgcg ctgtccatcg tgcgttgcgc gacctcgatc gatttcggcg tcttcagatg
       61 cggccgaagc tgcacgccca acgttccgag gtgtgcgcgc agccgctcga tgttgcgggt
      121 catccgtgct tcatccagca acagacacgg tgtgtccagt tcgtccagcg actgacggcc
      181 gccgctttca ttgcaaaact cactcattgc gcgtcgctcc gcggctgaac agcacggact
      241 gtcgggatcc aattcgcaat gtcgtccatg ggtacgaccg gcctcggtaa tttcttgaag
      301 tcgaagcgag cgagaatggg cgaagtcagg cccggcgcat cgacgctcaa gatttgttcg
      361 ccagagaaga attcatcgaa tgcagcccgg aaatggccgc gcgacttcag cgcgacaacg
      421 cgcgcgctgg cgatatcgat gccaaacatt tcgaagaaca tcggctcgtg gcactggtgc
      481 cgtttgctga tgacgatgac ggttacgccg ccgatctgca gcgcaaccga cggtccgagg
      541 tcaaagctgc agccggccaa ctgtccgcgc cggccgacac ctttgccgtc atgcagccgc
      601 accacgcgcg ccggcacgtc gaacgggtct gaatagacgg tagtttcgct gcggttgaac
      661 cgggcgtgga acgtttcccc gatgccggcc tcatatgcgt ccgccacgag atccggatct
      721 gtgatgacac cgaggatcgc accacgcaca tcctgttcgt ggagtgcctt gagaatgaac
      781 ggcgtgttgc cgcgtccgcc gccgccagga ttgtcggcta cgtcggcaag cagcaccggg
      841 gttcgcgacg catcgtcgcc tgttgctttc gcgacgcgga ctgcctcgtc gagggacgtc
      901 agctgcggcg taaacgcggc acgctcttgc cacgagtatg acgcaagatc gtgcgcaatc
      961 tgggccgcga gctgcgcatc gccgcgcgtg gtgacaagta ccgttagacc attcttcggt
     1021 gtatccgcaa atgcgaatcc gcctgcaacc gagatgttca cgatgcgcgg atcatcgagc
     1081 gcttcagcgc gtcggatcat ttctgcatag ggccctgtgc cgggcgctgt cagcaactgc
     1141 gtcggcggtg aacagacggg cattcgcaca tgcgcgacgg acgtgcgcat accgtccagg
     1201 agttcgatca tgatccgcgc cgcgtcggcg cctcgctcgg ccatatcggt gtgaggattg
     1261 cgacggtacg atacgagcgc atcgactgcg tcgaccattc ggtccgaaat gttggcatgc
     1321 aggtcgacgg tggcgacgat cggcaccgct ggcccgacaa tgctgcgtac gagcgtgaaa
     1381 accgcgccgt cgggatcgtc ttcttctgtc gtgatggctg cgccgtgctc acagatgtag
     1441 acggcgtcaa gcggcaacgc cgcccgcagc cgctgctcga attcacgcag cgtatcggcg
     1501 aagaatccgt gttcgaccgg tccgccagac tccgcgttag caaagaggat cgggaccggc
     1561 gtccatggtc ggatcgcatc cattgttcgg acgaacgccg ggatttccgg cgtcatcacg
     1621 ggtgccgcgc ttttcgcatc agccagaagc gcggcgtctt gcagatacgc acgcgatacg
     1681 aaatcggcgc gtgtggaaac cggcgcaaac cggttcgact cgatcgcgaa gccaagaatg
     1741 gcaatacgcg ggcttttcat taaacactcc ttatcggccg gcgtcttcgg ctggcattgg
     1801 gcgctgacgt ctcggcgggt cattgaagct aagttaccat cgacagcacg gcggcgtggc
     1861 gttggtgtac acgaacatcg gcacttgagt tcgcgcgcgc ggtgcaaaga gtattggcca
     1921 ccaaatagtt gtgcgggtta acatcacctt attgcttcgt taacctgtaa tttaatgatc
     1981 tcgaccgaag acctgcattt tttcatcacg gtcacggcgt ccgcttcgct cgcggacgcc
     2041 gcgcgcaagc tcgatgtgac gccgcccgct gtcacacaac gactgcgttc tctggaaaag
     2101 cggctgggaa tgcgtttgat cgatcgctcg gggcggcacc tggtactgac cgatgagggc
     2161 gaactgtttg cgctccacgc gcgcaggatc tgcgatgaca tcggcaatct atcggacacg
     2221 ctggcgtcgc gacgtgggac ggtcgcggga catcttcgag tgattgcgcc gctcggcttc
     2281 gggcagcgat atgtcgcccc cgccgtcgcg caattccgct gcctctatcc ggaagtgacc
     2341 gcaagcttga tgctgtccga tcgacccgcg cacatttcgg acgacatgtg ggacctgatg
     2401 gtccatatcg gcgagctacg cgattcgaca ctggttagcc ggcggctcgt gccgaaccgg
     2461 cgcgtcctgt gcgcggcacc gcaatatctc gcgcagcgcg gcgaaccacg gcacccggac
     2521 gatctgcggg tgcacgactg cattgcgcta cgcgaaaacg atgaagatgt gacgatgtgg
     2581 cgcttcatgc cgaagcacca cggcacaccg gcgaatgtca ggatcgaacc gttcctgacg
     2641 agcaacgacg gcggcaccgt gcgcgattgg gcacttgccg gactcgggat cattgtgcgt
     2701 tccgagtggg acgtcgccga acatgtccgt gcgggtcgac ttgtcccact gctcgatgag
     2761 tggcaattgc cggatgccga tatcgtcgcg ctgattagct cgcgacatgg acgctcattg
     2821 cgcgctacgc gctttctcga ccatctgcac tcgtcgttat cgcaactgcg ttagctgcca
     2881 tccgtcgtgg ccgaatctgc cgcggcgggt cacaatcttc cgctactccc cgggcatgag
     2941 gcaaccgcgc ggcttgaaga agcgccgcgc cggatgtctc gtactccaga aaaagtatcg
     3001 ggtggcagac tgacccgatc gactaccaag gccaggctag cgaggttccc acgccggttt
     3061 cacgggcacg ctcacacagc atgcgcgcca cggtcaggtc ctggagtgcc aggcctgtca
     3121 tatcgaacac cgtaatgtcg tcttcgcggc ggttcgcctg tgccttccct gtcagtagat
     3181 cgcccagttc cgtgcacggg tggtcggccg cccattgcgt ttcaccgatc tggcgcgcct
     3241 ggacttgatc gtcgacgaat agacgaacgc gccccaacag gccctcggga agttcacgct
     3301 ttcctttggt gtcggcgcct acgcaattaa ggtgcgtgcc gggttgaacc gagtgcaaat
     3361 cgaacagcgc tccagcacct ggggtagccg tgattacgat gtcgctttca ctaactgcac
     3421 gatcaccgtc attcgcgtgc gagatgtcgc agcgctgctc gaagtgagct tcgaacgcgg
     3481 ggtcacgctg gccattcgca gtcaggtagc gaacacgaac aagcgatggc atgagctgca
     3541 gagcgaaggt cagttgcacg cgcgcttgta cgcccgaacc gaagacgcac actcgagtgc
     3601 tgtccgcgcg tgcaaggtgc agtaagccaa gtccgccagc cgcacccgtg cgcatggtgg
     3661 tcaccgcatt gccgtcgatc atgcagagag gccgacccgt agccggatcg atcaacatga
     3721 tggttgcctg gtgaggctca ccattcagtg cacgattggt aggccagaat cccgctgcct
     3781 tgaaacccag cagcccttgc gccggaacat cgcctgattt gataccgaat atgccgcccg
     3841 tgaccaacgg ttccctgacc acgcgaaaaa cacggccttc gcgctggcta tgcagaacaa
     3901 acgcctcgcg caccgcttgc atcacatcat cgggcaacag caatgactcg acttcattct
     3961 tgtcgaggag caacaattga gcgtcacttg gcatcgttgt acaccgtagc gcgcgatacg
     4021 cccaaatgtt gcgcgacgac ctccatcgat cgacgtacgt ccatgctgcc cgactcccgg
     4081 agctctttca tcaaaatccg cctttcctct gtcttcaatg ctctgggtgt cgtcgcgagc
     4141 cgcgcggcga attggtctat ccgcattctt attccttccg ccccaggggg gtcgagcttt
     4201 tcattcgtac cggcagaatc gtccgtgcgg gcgaactgcc ccagcacgtt ctgcaaagca
     4261 ccaaaaagcg tgagatcgat attgatgcac agagcggcaa cgtactgccc tttcgagtcc
     4321 ttgatgccga ttgacgtgct ttttgcctgc cggccgtcgg cgaagcgatt tgcgtaattg
     4381 gcgaccactt gcggatacgc cgcatcaccg atgcgcgcca agccaagttc cgtggcagga
     4441 tctcctacct cacgacccga caaattgttg tgaatcgccg cgatggcgtg ctcggcgtcc
     4501 agcaggtcat gcacaactac ttcgcaaaac ggcgcaaacg tctcacccaa gcccagcgcg
     4561 acttgcttca gttgtgcgac cagcacctgc tgttctttcg tttgatttga catttgaaca
     4621 atatatccaa cattagacac gtcgtcaaca cgcgagcctc agtgtgcact tcggcgggtt
     4681 tttgcttgtt ctgaacgagc cgtcagtatg cgaccttgac ggtcggcgcg tcctccggac
     4741 agcgcctgcg ccggctgtag atctgcgcgg tcgcgatgtt cgcgcggccg ctcggtaccc
     4801 ggctcgttgc ccatgcgcac tcgaaattcc gggagcaaac gctagttcgc gccggcttac
     4861 aagcctttat ccccaacgtc cggcaacctc cgcttcgcac cgtcacgctt cgcaagtcgt
     4921 acttccgtag aaccggatgc cctatggcag gctgccggaa aatgcgttat gacgagcgat
     4981 gacaaagctt acaaattcca tgaccgattc tggaattgct cgtccctgcc gggcgatgat
     5041 gcccgatgtc gtttgagcca atcgctcatg ctgcaatgga attaacttaa atatggattc
     5101 ccagccactg gttttaattg caaattcagg caagatggtt attgcgagct tgttttttac
     5161 aagtgatttc aatatttcat aagagttact ttcaatcacc ggctgcaact tgatcccgac
     5221 tgcatcctcc actactttta tactctgtcg aatacgatgt tgcgagggca gaaggccaat
     5281 cgcaaattgc tgaagatcgt ccaggctcac cgattcggct gacgacaagg catgtcctag
     5341 cggaagaacg accaggagcg gcgccggcgc ggaatgaaca accttgataa ggtgatgctg
     5401 cggtggattc atggttattc caatatccac aacttcatcg attaccatcc tcaatacggc
     5461 atcgccgttg cctatgtcga taccgtacgt catttgcgga tgacgcgcta aaaattctgg
     5521 tattacagca tcggctaaat ctggaacata tccatttcct gatgccactt tcacatgccc
     5581 gaattgcaag ccgttcgagg tgcgaatggc gtcctttacc gcggcgtcct gcagcatgag
     5641 ctgacgaata tgctcacgca atatgtttcc cgcccctgtc agcacaatgc gtcggccgtg
     5701 ctgctctatc agggcctgtc caaactcgct ttctagcaat ctgacctgac ggctaagcgc
     5761 tgaagcggaa atgtggacac gttccgcggc tcgccgaatt gaaccctcca gagcgaccgc
     5821 gtaaaaatac cgataagcag aattcaacat gattggcgta atcagctagc ctggatttca
     5881 cgattgtgag ggaagcgcct cgggcgtacg gcagccagag actcgcggcg atgtattcga
     5941 ttgggacacg agatggctct ggatacatac ctttccagag cgcggcaagg gtcagacgca
     6001 acgatagcac cgatggagca agagcctgcg gagcctatct tctcgacgca gcatggagga
     6061 ggattgcgcg cctcctgcga gcatcacggt gcggttcctt gttcaaggtc agatgccgcc
     6121 gccgcttcat cgacggatgc acgacaccta tcgcgcgtcg actgagggtc gaagggcccg
     6181 ctgtcagggc cgtgatgcac cgccaaatct gacagaaacg gttgcgacac aaccaaccgg
     6241 ctttgagcag acggaatgcg cttctggtgt ccgggcaagc ccggtgctca aaagccctcc
     6301 gcccgagaaa ccagccacac gcgccgcatc cgatccctgg ctgacgcgat agcgctcaaa
     6361 aattagaaca gtcgcatcca ataattcgcc ttgaccggtt ctttt
//
LOCUS       DATASH010001001         3674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5712322,
            whole genome shotgun sequence.
ACCESSION   DATASH010001001 DATASH010000000
VERSION     DATASH010001001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5712322"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(347..1369)
                     /gene="galE"
                     /locus_tag="VHV99_26665"
     CDS             complement(347..1369)
                     /gene="galE"
                     /locus_tag="VHV99_26665"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEX3639001.1"
                     /translation="MTTKGTILVTGGAGFIGSHTCVELLNGGYDVVVIDNLVNSNRES
                     LRRVEEITGKPVTFYEADARDEAALNRIFDAHQITGAIHFAALKAVGESVAKPIEYYS
                     NNVGSLLTLLGVMRERNVKQFVFSSSATVYGVPKSSPIDESFPLSATNPYGQSKLIAE
                     QVLRDLEVADPSWRIATLRYFNPVGAHKSGLIGEDPAGIPNNLMPYVAQVAVGKLEKL
                     RVFGGDYDTPDGTGVRDYIHVVDLARGHLAALDALVKRDASFVVNLGTGKGYSVIDVV
                     KAFEKAAGRPVPYEIVARRPGDVASCFADPAAAEKIIGWRAQFGIERMCADHWRWQST
                     NPQGFA"
     gene            complement(1382..2530)
                     /locus_tag="VHV99_26670"
     CDS             complement(1382..2530)
                     /locus_tag="VHV99_26670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX3639002.1"
                     /translation="MKPALKSTPALRITLVCNTAWAIYTYRQGLIRMLVGRGVDVTVL
                     APRDRTFDLLTAMGCRCIELPVASKGTSPRDDLRTLLSLYRLYRSIRPHVVFHYTIKP
                     NIYGSVAAKLAGVQSVAVTTGLGYVFIQHSRAALVAKKLYRFAFRFPREVWFLNRDDQ
                     AAFVEQNLLVHPERARLLHGEGVDLEQFAFTPLPERAEFRFVLIGRLLWDKGVGEYVE
                     AARRVRERYPQARFQLLGPVGVDNPSAITREEVAVWERDGIIEYLGEAHDVRPFIAEA
                     DCVVLPSYREGVPRTLMEASAMGRPIVTTDVPGCREVVADGVNGLLCEVRNAESLARK
                     LAQMLDMSGAERRAMAERGRQKVAQEFDERGVVETYKDLVQKMTGVLL"
     gene            complement(2592..3419)
                     /locus_tag="VHV99_26675"
     CDS             complement(2592..3419)
                     /locus_tag="VHV99_26675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3639003.1"
                     /translation="MTNSSAAAHALDDVAVLMPAYNGQADVDLTLASFSESALVHVLI
                     VDDGSATPIVAPAIPNMKIEVLRMSRNSGIERALQTGIDALAQRGFRYAARIDAGDRS
                     VPQRLTRQRRFMELHPQVAGLGMWTQVVTREGKPLFMLTPPAEPDQIRRLRFFRSCLA
                     HPSMMLRVDAVRAAGNYRAAYPSAEDLDLFLRLMERYDCANLPELGLYYELNEGGISA
                     TKRRRQVNSTLRLQLRYFNAANLYDWLGLAKNLLHLVTPYRALQRVKRTLLAPRASQ"
     gene            complement(3416..>3674)
                     /locus_tag="VHV99_26680"
     CDS             complement(3416..>3674)
                     /locus_tag="VHV99_26680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018431750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HEX3639004.1"
                     /translation="IDIFRWAVWLSVPAGIEAIGNLIVLKYQAKFVLLSKWLLALAVA
                     FAVNLLAIPRLGAYGALVGLAAGYLAAASVNLYYIRFKLRP"
BASE COUNT          711 a         1176 c         1125 g          662 t
ORIGIN      
        1 cggcttgccg gttcatagct tccgtacgct gctcgatgac ctggccacac tcacctacaa
       61 catctgccac acgccgctga atccggacgc cagaatcgtc atgtccacgc ggcccacacc
      121 ggttcaggaa aaggcctttc gccttctgaa cgtcaacccc gattgtaccc agtagccgtc
      181 cccttcgcgc gcgcataacc gtgataaatc aaaggattat gcgcgccggc acccaaagct
      241 cggattagta atatgcgtaa ttacccgctt ggtgcaatcc cgcaatcgac ggcggcggag
      301 cccatgcctg gcgactcctt cgcgccgccg tcgagcttcg ttcaactcag gcaaatccct
      361 gcggattcgt ggactgccag cgccagtgat ccgcgcacat ccgctcgatg ccgaactgcg
      421 cgcgccagcc gatgatcttc tcggccgcgg ccggatcggc gaaacacgag gcgacgtcgc
      481 cggggcggcg cgccacgatt tcatacggca ccggccggcc ggcggccttc tcgaatgcct
      541 tgaccacatc gatcacgcta tagcccttgc ccgtgccgag gttcaccacg aagctggcgt
      601 cgcgcttgac cagggcgtcg agcgcggcca gatgtccgcg cgccagatcg acgacgtgga
      661 tatagtcgcg cacgccggtg ccgtcgggcg tgtcgtagtc gccgccgaac acgcgcagct
      721 tctcgagctt gccgaccgcc acctgcgcga catacggcat caggttgttc ggaatgccgg
      781 cgggatcttc gccgatcagg ccgcttttgt gcgcgccgac cggattgaag tagcgcagcg
      841 tggcaatgcg ccacgacgga tcggccactt ccagatcgcg caatacctgc tcggcgatca
      901 gcttggactg gccgtaggga ttggtggccg acagcgggaa tgattcgtcg atcggcgagc
      961 tcttcggcac gccgtagacg gtggccgacg aactgaacac gaactgcttg acgttacgct
     1021 cgcgcatcac gccgagcagg gtcagcaggc tgccgacgtt gttgctgtag tactcgatcg
     1081 gcttggccac cgactcgccc accgccttca gtgcggcaaa gtgaatcgcg cccgtgatct
     1141 ggtgggcgtc gaaaatacgg ttgagcgccg cttcgtcgcg cgcgtcggct tcgtagaagg
     1201 tcacaggctt gccggtgatt tcctccacgc gccgcagcga ctcgcggttg ctgttcacga
     1261 gattgtcgat taccacgacg tcgtaaccgc cgttcagcag ttcgacacag gtgtgcgagc
     1321 cgataaatcc tgcaccacct gttaccagaa tcgtaccctt cgtggtcatg ctgttactcc
     1381 cttaaagtaa aacacccgtc attttctgca cgaggtcttt atacgtttcg acgacgccgc
     1441 gttcgtcgaa ctcttgcgcg actttctgtc gaccgcgttc ggccattgcg cggcgctcgg
     1501 cgccgctcat gtcgagcatc tgcgccagtt tccgagcaag actttctgca ttgcgtactt
     1561 cgcacagcaa tccattgacg ccgtccgcca ccacctcacg gcagccgggc acatccgtcg
     1621 tcacgatcgg ccggcccatc gccgatgctt ccatcagcgt gcgcggcacg ccttcccgat
     1681 atgacggcag gacgacgcag tccgcctcgg cgatgaaggg gcgtacgtcg tgcgcctctc
     1741 caagatactc gatgatgcca tcacgctccc acacggccac ctcttcgcgt gtgatcgcgc
     1801 tcgggttgtc gacgccgacc ggaccgagca gctggaaccg cgcctgcggg taacgttcac
     1861 gcacacgccg tgcggcttcg acatattcgc cgacgccctt gtcccacaag agccgcccga
     1921 tcagcacgaa cctgaattcc gcccgctccg gcagcggcgt gaaggcgaac tgttcgagat
     1981 cgacgccttc gccatgcaac agccgcgcac gctcaggatg caccaacaaa ttctgctcga
     2041 caaacgcggc ctggtcgtca cggttcaaaa accagacttc acgcggaaaa cggaaggcga
     2101 aacggtacag ctttttggcg acaagcgccg cgcgactgtg ctggatgaac acgtaaccga
     2161 gcccggtagt caccgccacc gactgcacgc ccgccagttt cgcggcgacc gagccgtaga
     2221 tgttcggctt gatcgtgtag tggaacacga catgcgggcg gatgcttcga tacaaacgat
     2281 acaacgacaa aagcgtgcgc aggtcgtcgc gcggactggt gcctttggac gccaccggca
     2341 attcgatgca gcggcacccc atcgcggtga gcagatcgaa ggtgcggtcg cgcggcgcga
     2401 gcaccgtcac gtcgacgccg cgtccaacca gcatgcggat cagcccttgc cgatacgtat
     2461 agattgccca ggcggtatta cagacgagtg taatgcgcag cgcgggcgtc gacttcaagg
     2521 caggcttcat gcgggaacgg gctcggcggt gaatgacggg gtgaaatgag cgggtgagtg
     2581 acgcaatgga atcattggct ggcacgcggc gcaagcaacg tgcgtttgac ccgctgcaag
     2641 gcgcggtaag gcgtcaccag atgcagcaga ttcttggcga gacccagcca gtcgtacaga
     2701 ttggcggcgt tgaagtagcg cagttgcagc cgcagcgtcg agttgacctg gcggcgccgt
     2761 ttggttgcgc taatgccacc ttcgttcagc tcgtaataga gcccgagctc cggcagattc
     2821 gcgcagtcgt agcgctccat caaacgcagg aacaggtcga ggtcttcggc cgaaggatac
     2881 gccgcgcgat agttgccggc cgcgcgaacg gcgtcgacgc gcaacatcat cgaagggtgc
     2941 gcgaggcacg aacggaagaa gcgcagacgc cgaatctggt ccggttcggc gggcggcgtc
     3001 agcatgaaaa gcggcttccc ttcacgcgtg acgacctggg tccacatgcc gaggccagcc
     3061 acttgcggat gcagttccat gaacctgcgt tgtctggtga gccgctgcgg cacgctgcgg
     3121 tcgcccgcgt cgatgcgggc cgcgtagcga aaaccgcgct gcgcaagggc gtcgatgccg
     3181 gtttgcagtg cgcgttcgat gccgctgttg cgcgacatac gcagcacttc gatcttcatg
     3241 ttcgggatcg ccggcgcgac gatcggtgtc gcgctgccgt cgtcgacgat cagtacgtgg
     3301 accagcgcgc tttcgctgaa cgaggccagc gtgagatcga cgtcggcctg gccgttgtac
     3361 gctggcatca ggacggcgac gtcgtcgagg gcatgcgctg cggcggatga attcgtcatg
     3421 ggcgcaattt gaaacggatg taataaagat taactgacgc ggcggcgaga tagccggccg
     3481 cgagtcccac cagcgcaccg taagcgccga gccggggaat cgccagcaga ttgacggcga
     3541 atgcgaccgc cagcgccaac agccacttgg aaagcaggac gaacttggcc tgatatttca
     3601 gcacgatcag attgccgatc gcttcgatcc cggccggcac cgaaagccac acggcccagc
     3661 ggaaaatgtc gatg
//
LOCUS       DATASH010001002         2229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3532906,
            whole genome shotgun sequence.
ACCESSION   DATASH010001002 DATASH010000000
VERSION     DATASH010001002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3532906"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VHV99_26685"
     CDS             <1..199
                     /locus_tag="VHV99_26685"
                     /inference="COORDINATES: protein motif:HMM:NF013653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEX3639005.1"
                     /translation="ESSGNRLRVISASTDCDNDALLFKVEVEGDGLVCHEGTVSCFTK
                     PIAVSPNAADTTNDTANGATK"
     gene            199..1077
                     /gene="hisG"
                     /locus_tag="VHV99_26690"
     CDS             199..1077
                     /gene="hisG"
                     /locus_tag="VHV99_26690"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEX3639006.1"
                     /translation="MSQKKLKLGIPKGSLQDATIALFERAGWRIFASGRSYFPTIDDV
                     EIECMLVRAQEMARYVEHGALDAGLTGRDWILENQSDVEYVTSLTYSKQSRQTVKWVL
                     AVPEDSPFQKPEDLAGKIIATELVEFTKRYFASKNIPVTVEFSWGATEVKPPTLADAI
                     VEVTETGSSLRANRLRIIETLMESETQLISNKTAYQDAWKREKIDNISLMLNAAIAAQ
                     GRVGLLMNARKADLETITAVLPALNSPTVSELRDPEWVALNTILDETQVRDVIPKLKL
                     AGATGIVEYPLSKVVL"
     gene            1190..>2229
                     /gene="hisD"
                     /locus_tag="VHV99_26695"
     CDS             1190..>2229
                     /gene="hisD"
                     /locus_tag="VHV99_26695"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX3639007.1"
                     /translation="MSTASVEPVVRKILAAVEKRGERAVLEYATKLDGLAKSQPLLVA
                     REEMRAAWESTDPKLQEAMRIAQANIRAFAEAQKASEWTIDRDGVKTGQIVRPLASVG
                     CYVPGGRYPLPSTLLMTVTPAQVAGVERIVVCSPKPARETLAAAWLAGVTEFYRIGGA
                     QAIAALALGTTTPTGTTGTGGSIAPVDKIVGPGNLYVTAAKMAVSHRCGIDMPAGPTE
                     IVVTSETGKAADIAADLVAQAEHDPETLAVMITTNVELATATAAEVKLQVKANPLAKQ
                     SIAARGCIFVTKTITEARELTNRLAPEHLTVDSAADLKWVRNAGSVFVGPYSPQSMGD
                     YISGPNHVLPTGR"
BASE COUNT          443 a          718 c          732 g          336 t
ORIGIN      
        1 cgagagcagc ggcaaccggc tgcgcgtgat ctcggcctcc accgactgcg acaacgatgc
       61 gctgctcttc aaggtcgagg tcgaaggcga tggcctggtc tgccacgagg gcaccgtgag
      121 ctgcttcacc aaaccgattg ctgtctcccc caacgccgca gacaccacga acgacaccgc
      181 caacggagcg accaagtaat gtctcagaag aagctgaagc tcgggattcc caagggcagc
      241 ctgcaggatg ccaccatcgc tctgtttgaa cgcgccggct ggcgcatctt cgccagcggc
      301 cgcagctact tccccaccat cgacgacgtc gagatcgagt gcatgctcgt ccgcgcgcag
      361 gagatggccc gctacgtcga gcatggcgcg ctggacgccg gcctgaccgg ccgcgactgg
      421 atcctcgaga accagtccga tgtggaatat gtcaccagcc tgacctactc caagcagagc
      481 cggcagacgg tgaagtgggt gcttgcggta ccggaggatt cgccgttcca gaagcccgag
      541 gacctcgcag gcaagatcat cgccaccgag ttggtcgagt tcacaaagcg ctacttcgcg
      601 agcaagaaca tcccggtcac ggtggagttc agctggggtg cgaccgaggt gaagccgccg
      661 acgctggccg atgccatcgt cgaggtcacc gagaccggct cgagcctgcg cgccaaccgc
      721 ctgcgcatca tcgagacgct gatggagtcc gagacgcagt tgatctcgaa taagaccgcc
      781 tatcaggacg cgtggaagcg cgagaagatc gacaacatct cgctgatgtt gaatgccgcg
      841 atcgccgcgc agggccgtgt gggcctgctg atgaacgcgc gcaaggcgga tctcgaaacg
      901 atcacggcgg tgctgccggc gctgaactcg ccaaccgtct cggagttgcg cgatccggag
      961 tgggtggcgc tgaacacgat tctcgatgag acgcaggtgc gcgacgtgat tccgaagctg
     1021 aagctcgccg gcgccactgg catcgtcgaa tacccgctga gcaaggtcgt actctaacgc
     1081 tgcatcgctt caacaaagag ggcaacgcac gatgaaggta gtacgcacat ttggacgcag
     1141 cgcgcagacc gccgaggagc tgatccagag cctcgaacag cgcggcgcgg tgagcaccgc
     1201 aagcgttgag ccggtggttc gcaagatcct cgccgcagtc gaaaagcgcg gcgagcgcgc
     1261 cgtgctcgag tacgccacca agctggacgg tctggcgaag agccagccgc tgctggtcgc
     1321 acgcgaggag atgcgcgccg cctgggagag caccgatccc aagctgcagg aggcgatgcg
     1381 catcgcgcag gccaacatcc gcgcctttgc cgaggcgcag aaggccagcg agtggacgat
     1441 cgatcgggac ggcgtgaaga ccggccagat cgtgcgtccg ctggccagcg tgggctgcta
     1501 cgttccaggt gggcgctacc cgttgccctc gaccttgctg atgacggtga cgcccgcaca
     1561 ggtggctggc gtggagcgca tcgtcgtctg ctcgccgaag cccgctcgcg agacgctggc
     1621 cgccgcatgg ctggctggcg tcaccgagtt ctatcgcatc ggcggagcgc aggccatcgc
     1681 cgcgctcgcg ctgggcacga ctactccaac aggcacaacg ggcaccggcg gcagcatcgc
     1741 gccggtcgat aagatcgtcg gtccgggcaa tttatatgta accgcggcga agatggcagt
     1801 aagccaccgc tgcggcatcg acatgccggc gggaccgacc gagatcgtcg tcaccagcga
     1861 gaccggcaaa gccgcggaca tcgccgccga tctggtcgcg caggccgaac atgatcccga
     1921 gacgctggcc gtgatgatca ccaccaacgt ggagctggcc acggccaccg ccgcggaggt
     1981 gaagctgcag gtgaaggcga atccgctggc gaagcagtcg atcgccgcgc gcggctgcat
     2041 ctttgtgacc aagacgatta cagaagcccg tgagctgacc aaccggctcg caccggagca
     2101 tctgaccgtc gactccgccg ccgacctgaa gtgggtgcgc aatgcgggct cggtgttcgt
     2161 cgggccgtac tcgccgcaat cgatgggcga ctatatttcg gggcccaatc acgtgcttcc
     2221 caccggccg
//
LOCUS       DATASH010001003         3425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6498969,
            whole genome shotgun sequence.
ACCESSION   DATASH010001003 DATASH010000000
VERSION     DATASH010001003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6498969"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..262
                     /locus_tag="VHV99_26700"
     CDS             11..262
                     /locus_tag="VHV99_26700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004529359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639008.1"
                     /translation="MRTTGSSGSMALLTEYDDATARELRSLRLESTEDGKGILLIEVD
                     ERKPGIHREVRYEITPAELIAAIRAHGAELPGEQHNYRQ"
     gene            610..1602
                     /locus_tag="VHV99_26705"
     CDS             610..1602
                     /locus_tag="VHV99_26705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein/thiosulfate
                     sulfurtransferase GlpE"
                     /protein_id="HEX3639009.1"
                     /translation="MLHDLVEQYGPALVFVNVLAASLGLPVPAMPSLVLFGAMAAMHP
                     GSVGTQLLPVLILSIFATLIGDSAWYLAGRMYGGNTLKTICRLSLSRDTCVKKTERFF
                     GRWGVRVLAVAKFVPGLSIVSIPMAGAMGTRYRTFLTYDSIGAALWSGTGLIIGALFA
                     RQIDMLFAMAGRLGRTAALVAVALLLLYAAYRWIRRRQLISRLATQRIEVDELAALVA
                     AGKTPVLFDIRSHEKRVLDPFVIPGSQFADERQLDEIVATYPHDQKVVIYCSCPNEIS
                     AAWMAKQMSEAGFADVLPLRGGMEAWRDSGKTVDALPHTPPPHVAVDDIAPKAV"
     gene            1696..3408
                     /locus_tag="VHV99_26710"
     CDS             1696..3408
                     /locus_tag="VHV99_26710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3639010.1"
                     /translation="MLSTQETEGQQAIVADAPFSSLATRMHQMFPELTCAEIDRLRRF
                     GEVGHWEAGELLFETGHTGPGMFVLLNGRVKVYQRDGIGREVLIAEHGAGHFLAEVGQ
                     LSGRPALVNGMAVGAVDALLIPPAKLRALIVAEAELGERIMRALILRRVSLIEKGAGG
                     PILIGNSSDARLVMLQGFLSRNGHPHSVIDERDEDALRLIEQFAAQREDMPLVICPDG
                     SVLRHPSMPELATCLGLLPDLDDSHVYDVAIVGAGPAGLATAVYAASEGLSVIVLDSR
                     APGGQAGASSRIENYLGFPTGISGQALAGRAFVQAQKFGAHVAIPVHVKALHCAESPY
                     RLELKCAGHITARTIVIASGAVYRRPALKGLDRFDGRGVYYLASPVEAKLCKRQEIVI
                     VGGGNSAGQAIVYLATHAAKVHVLIRRSGFEATMSRYLIDRIRSLPNVFVHPHSEVGQ
                     LEADEIGLASVSLKKPLPDGTTSIDTRHLFLFTGADPNTDWLRTCGVELDDKGFVLTG
                     TSADGISVCDLATTVEGVYAIGDARAGSTKRVAAAVGEGAAVVAQIHQLLAVSAGEAV
                     ALGA"
BASE COUNT          579 a         1091 c         1100 g          655 t
ORIGIN      
        1 gacaacttcg atgcgcacta ccgggtcctc cgggtcaatg gccctgctca ccgaatacga
       61 cgacgcaacg gcgcgcgaac tccgctcgct gcgcctcgaa tcgacagaag acggcaaagg
      121 cattcttctg atcgaggtcg atgagcgcaa gccaggcatt caccgcgaag tgcgctatga
      181 aatcacgcct gccgaactga ttgcggcgat tcgcgcgcat ggcgcggagc tgccgggcga
      241 acagcacaat tatcgtcagt aaaactgcca cgcccggcat tgcgctcacg cgcgaatgcc
      301 gggtgcagcc tgcctctaca cgcccgctct cgtttgggca ctgcaggatc tctttctcct
      361 acgggcgtcc gtagggcgtt attcgccacc cgctagccgc gcacgcttca atccatttcg
      421 atttgttcca ttcgtgcttc tatccgcgct tttaatcctg cgggtatacc ggagacgcgt
      481 ttttaacctc ccgattgcgg acccgttttc cgggcgtttg cacgtcttga aaatgcgtcc
      541 ataatccttg cattctcctt tgcttttccc tacccgcgcc gttcccgaaa gggcgacgga
      601 agtaagccga tgctacatga ccttgtcgag caatatggac cggcgctcgt cttcgtgaac
      661 gtgctggccg cctcgctcgg cctgccggtg cccgcgatgc cgtcgctcgt gttgtttggc
      721 gcgatggcgg ccatgcatcc cggctcggtg ggcacgcaat tgttgccggt tctgatcctg
      781 tcgatattcg ccacgctgat cggcgacagc gcctggtatc tcgccggccg catgtatggc
      841 ggcaatacgc tgaaaacgat ctgccgtctt tcgctctcgc gcgacacctg tgtaaaaaag
      901 accgaacgct ttttcggccg ctggggcgtg cgcgtgctgg ccgtggcgaa gttcgtgccg
      961 ggcctgtcga tcgtgtcgat cccgatggcc ggcgcaatgg gtacgcgcta ccgcacattt
     1021 ctcacttacg acagcatcgg cgccgccctg tggtcgggta ccggtttgat catcggtgcg
     1081 ctgttcgccc gccagatcga catgctgttc gccatggccg gacgcctcgg gcgcacggct
     1141 gcgctggtgg ccgtcgcgtt gctgctgctg tatgcggcct atcgctggat tcgccggcgc
     1201 caactgattt ccaggctcgc gacccagcgg atcgaagtcg atgaactcgc agcgctggtc
     1261 gcggccggca aaacgccggt actgttcgat atccgttcgc acgagaagcg cgtgctcgat
     1321 ccgttcgtga ttccgggctc gcaattcgcg gacgagcggc aactcgacga aatcgtcgcg
     1381 acgtatccac acgatcagaa ggtagtgatt tactgttcat gcccgaacga aatatccgcg
     1441 gcatggatgg ccaaacagat gagcgaagcc ggcttcgccg acgtgctgcc gttacgcggc
     1501 ggcatggaag cctggcgcga ctcgggcaag acggtagacg cgctgcccca tacgccgccg
     1561 ccccacgtgg cggtcgacga tatcgcgccg aaggccgtgt agcgcagcag cgcaccccta
     1621 tcggcgccct cgcgcgcccg cgccgcttcg ctacccgtgt cgatcaatcc ggataaacac
     1681 agaaggagag gttcaatgct gtcgactcaa gaaacagaag ggcaacaagc catcgtcgcc
     1741 gacgcgccgt tttcgtcgct cgcgacacgc atgcaccaga tgttccccga actcacgtgc
     1801 gcggaaatcg atcgtctgcg ccggttcggc gaagtgggcc actgggaagc cggtgagctg
     1861 ctgttcgaga ccggccacac cggaccgggt atgttcgtgc tgttgaatgg acgcgtgaag
     1921 gtttatcagc gcgatggcat cgggcgcgaa gttttgatcg cagaacatgg cgccgggcat
     1981 tttctcgccg aagtcggtca gttgtccgga cggcccgcgt tggtcaacgg catggccgtc
     2041 ggcgcggtcg acgcgttgct gatcccgccg gccaagctgc gcgcactgat cgtcgccgag
     2101 gccgagctgg gcgagcgcat catgcgcgcg ctgattttgc ggcgcgtgtc gctgatcgaa
     2161 aagggcgcgg gcggcccgat cctgatcggc aacagcagcg acgcgcggct cgtcatgctg
     2221 caaggcttcc tgtcgcgcaa cggccatcct cattccgtca tcgacgaacg cgacgaggac
     2281 gcgctgcgtc tgatcgagca attcgccgcg caaagagaag acatgccgct ggtgatctgc
     2341 cccgacggtt cggtgctgcg tcatccgagc atgccggaac tcgccacctg cctcggcttg
     2401 ctgcccgatc tcgacgattc gcacgtgtac gacgtcgcga tcgtcggcgc gggaccggcc
     2461 ggcctcgcca cagcagtata cgcagcgtcc gaaggcctct cggtcatcgt gctcgacagt
     2521 cgcgcgccgg gcgggcaggc gggcgccagt tcgcggatcg agaactatct cggctttccg
     2581 acggggatct ccggccaggc gctggcggga cgtgcgttcg tgcaggcgca gaaattcggc
     2641 gcgcacgtgg cgattccggt ccacgtcaag gcgctgcatt gcgcggagtc gccgtaccgg
     2701 ctggaactca agtgcgccgg gcatatcacc gctcgcacca tcgtgatcgc gagcggcgcg
     2761 gtgtatcgac gccctgccct caaagggctc gaccgtttcg atgggcgcgg cgtctactac
     2821 ttggcgtcgc cggtcgaggc caaactgtgc aaacgccagg agatcgtgat cgtgggcggc
     2881 ggcaattcgg cgggccaggc gatcgtctat ctggcgacgc acgcggccaa ggtccacgtg
     2941 ctgatccgcc gaagcggctt cgaagccacc atgtcgcgct atctgatcga ccgcatccgc
     3001 tcgctgccga atgtattcgt gcatccgcat tcggaagtgg gccagctgga ggcggacgaa
     3061 atcgggctgg cgtcggtctc gttgaagaag ccgctgccgg atggcaccac gagcatcgac
     3121 acgcggcatc tgttcctttt caccggcgcg gatccgaata ccgactggct gcgcacctgt
     3181 ggggtggaac tggacgataa gggcttcgtg ctgaccggca ccagcgcgga cggaatctcc
     3241 gtctgcgatc tcgcgacgac ggtcgaaggc gtgtatgcga tcggcgacgc gcgcgccggc
     3301 tcgacgaaac gggttgcggc ggcggtcgga gaaggcgcgg cggtggtcgc gcagatccat
     3361 cagctgctgg ctgtctcggc gggcgaagcg gtcgctttgg gcgcctgagc ggccgcgttc
     3421 ggaca
//
LOCUS       DATASH010001004         2753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3731048,
            whole genome shotgun sequence.
ACCESSION   DATASH010001004 DATASH010000000
VERSION     DATASH010001004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3731048"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..980
                     /locus_tag="VHV99_26715"
     CDS             <1..980
                     /locus_tag="VHV99_26715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX3639011.1"
                     /translation="NRFLLIGLVVIAAFVAVLLFAVARLVGRPLAELTQASERIAAGD
                     LSVRLATTREDDIGRLMIAIDGIATGLARIVTQVRSASADMSDGTGRIAAGSSDISLR
                     IATQASSLEETAASMEQITSTVQQNAANTAQADQLVASASEAARAGGDAVGRVVATMG
                     EIDASARKISDITSVIEGIAFQTNILALNAAVEAARAGEHGRGFAVVASEVRALAQRS
                     SAAVKEIEALIAESVGRVADGYRIAEEARGTMNGIVERVARVNAIIGEISVASREQSS
                     GIEQVNLAVSQIGEATQQNAALVGEAEQSTAGLHEQAEQLSRAVSVFRL"
     gene            complement(1060..1680)
                     /locus_tag="VHV99_26720"
     CDS             complement(1060..1680)
                     /locus_tag="VHV99_26720"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX3639012.1"
                     /translation="MSDSNAVHFYNPDEGHKLRHDPFKAIVAPRVIGWISSCDPDGHV
                     NLAPYSFFGAFASFPHIIGFSSEGRKDSIANIEATGEFVWNLSSKPLAEKMNRSSAAV
                     PHEIDEFELAGLTKAPGVNVAVPHVAESPAALECKLLQIIRLKSLDGTPMNNWLALGQ
                     VVGVHIRREFLKDGLFDTAAAQPIMRAGYRGDYAGIGEMFEMIRPG"
     gene            complement(1677..2615)
                     /locus_tag="VHV99_26725"
     CDS             complement(1677..2615)
                     /locus_tag="VHV99_26725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX3639013.1"
                     /translation="MNQITLGVVGSGLMGVGIATQAALHGYLTIVHDIDAARLASVHG
                     KAEAILDELIDAGVFTADNKPDVLARIETTDQLERVATAGFVVEAIPEVLELKNRTYR
                     ALEPLLRDDAIFASNTSGFPPDLLAQVLQNKARFVIAHFWNPPHTIPLVEVVPGTETD
                     PDVTRRTADLMTAIGMEPVVLTKAIPGFVGNRLQFAVLREALAIVRSGAATAEVVDTV
                     MKASLGRRYGMVGPLESADLGGLDTLLDIGTHLMPELAKDEDVLQLMREHVQKGRTGA
                     RSGQGFYEWDDAHHARVRHGRKRLIHHTAKKTGKPS"
BASE COUNT          500 a          854 c          886 g          513 t
ORIGIN      
        1 gcaaccgctt cctgctgatc gggttggtgg tgatcgccgc gttcgtggcc gtgctgctct
       61 tcgcggtagc caggctggtg ggccggccac tcgcagagct gacgcaagcc tctgaacgca
      121 tcgcggcggg cgatctgagc gtgcggctcg cgaccacgcg ggaggatgat atcggccggt
      181 tgatgattgc catcgacgga attgcgaccg ggcttgcgcg catcgtgacg caggtacgca
      241 gcgcatcggc ggatatgtcc gacggcacgg ggcggattgc agcgggcagc agcgacatct
      301 cgctgcgcat cgcgacacag gcaagcagtc tcgaagaaac ggcggcgagc atggagcaga
      361 tcacgtcgac cgtgcaacaa aacgccgcta acacggcgca ggcggatcaa ctcgtggcat
      421 cggcatccga agccgcgcgc gccggtggcg acgcagtcgg ccgcgtggtc gcgacgatgg
      481 gtgaaatcga tgcgtccgcg cgcaagattt cagacatcac gtcggtgatc gaaggcatcg
      541 cgttccagac caacatcctg gcgttgaacg cggccgtgga ggccgcgcga gccggagagc
      601 acggcagggg ctttgccgtg gtggcgtcgg aggtgcgcgc gctggctcag cgcagctcgg
      661 cggcggtcaa ggaaatcgag gcgctgatcg cggagtcggt cggccgggtc gccgatggtt
      721 accggattgc ggaagaagcc cgcggcacga tgaacgggat cgtggaacgc gtcgcgcggg
      781 tgaacgcgat cattggcgag atcagcgtgg cgtcgcgtga acaatcgagc ggcatcgagc
      841 aggtgaacct tgccgtgtcg cagatcggcg aagcgactca gcagaacgcg gcgctagtcg
      901 gcgaagcgga acaatcgact gccggcttgc atgagcaagc cgaacagttg agccgtgcgg
      961 tgagcgtgtt tcggctctga tcgcgcgcgg cggcgggggc gtcgtggatg atccgaacag
     1021 ccccgcccgc ccggaatgac cgcggcgcct cggccggcgt tagcccgggc ggatcatctc
     1081 gaacatttcg ccgatacccg cgtaatcccc gcggtaaccc gcacgcatga tgggctgcgc
     1141 ggcggcggta tcgaagagac cgtccttcag gaactcgcgc cggatatgca cgccaactac
     1201 ttgcccgagg gcgagccagt tgttcatcgg cgtgccgtcc agggatttga gccggatgat
     1261 ctgcagcaac ttgcattcga gcgcggccgg tgactccgcc acgtgcggca cggccacatt
     1321 cacgcccggc gctttcgtga gtcccgccag ctcgaattca tcgatttcgt ggggtaccgc
     1381 cgcggacgag cggttcatct tttcggcaag cggcttgctc gacagattcc acacgaactc
     1441 accggtcgct tcaatattcg cgatgctgtc cttgcgcccc tcgctcgaga atccgatgat
     1501 gtgcgggaac gaagcgaacg cgccgaaaaa actgtagggc gcgaggttca cgtgcccgtc
     1561 cggatcgcag ctcgaaatcc agccgatcac tcgcggcgcg acgatcgcct tgaacgggtc
     1621 atggcgaagc ttgtggccct cgtcgggatt gtagaagtga accgcgttgc tatcgctcat
     1681 gagggctttc cggtcttctt tgcagtgtgg tgaatcaggc gcttgcgccc gtgtctgact
     1741 cgcgcatggt gcgcgtcgtc ccattcataa aacccttgcc cgctgcgcgc gcccgtccgc
     1801 cctttctgca cgtgctcgcg catcagttgc aatacgtctt cgtctttcgc cagctccggc
     1861 atcaggtgtg tgccgatatc cagcaacgta tccagcccgc cgagatccgc actttccagc
     1921 ggtccgacca tgccatagcg ccggccgagt gatgccttca ttaccgtgtc gactacctcc
     1981 gccgtcgcgg cgcccgaacg cacaatggca agcgcttcgc gcagcacggc aaactgcagc
     2041 cggttgccga caaaacctgg aattgccttc gtcagcacaa ccggttccat gccgatggcc
     2101 gtcatcaggt cagccgtgcg gcgcgtgaca tccggatcgg tttcggtgcc cggcacgact
     2161 tcaaccagcg gaatggtatg cggcggattc caaaaatgcg cgatgacaaa acgcgccttg
     2221 ttttgcagca cttgcgcgag cagatcgggc ggaaagccgc tcgtgttgct ggcgaaaata
     2281 gcgtcgtcgc gcagcagcgg ttcgagcgcc ctgtaggtgc ggttcttcag ttcgagcact
     2341 tcgggaatgg cttccacgac aaaacccgcc gtggccaccc gctccagttg atcggtagtt
     2401 tcaatacgcg cgagtacgtc cggcttattg tccgcagtga agacgccggc gtcgatcaat
     2461 tcgtcgagga tcgcttctgc ttttccatgc accgatgcaa gccgtgcggc gtctatgtcg
     2521 tgaacaatgg tcaggtagcc atgcagcgcc gcctgcgtcg caatgccgac gcccatcaag
     2581 ccgctgccca ccacgccgag tgtgatttga ttcaaatttt ccccgcaatg cttagattcc
     2641 aagcttcgat tcgatggcct gttcgggtcc gttcgggtcc cgacgggcca tgaagccgtc
     2701 cgatacggcc ttcaaggccg cgtctcttaa ggatagcgca gcgcctccaa tcg
//
LOCUS       DATASH010001005         4780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6699663,
            whole genome shotgun sequence.
ACCESSION   DATASH010001005 DATASH010000000
VERSION     DATASH010001005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4780
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6699663"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="VHV99_26730"
     CDS             <1..657
                     /locus_tag="VHV99_26730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX3639014.1"
                     /translation="YQSIFDGVLNHRLTPGMKLPEPELCQLFGVGRTVARRVLEKLAY
                     DGIVVLRPNKGAVIAEPTLEETREIFEARRSVERMLVELAVQRASAKDILALRQQLAS
                     EHEAMHRFDQPSWVTLASGFHMRIAALAGNSILQNYLKELVSRCSLIVGVYEPPGHAP
                     CEHAEHAAIVDCIETRDAAGAIAHMEAHLRDLEERIETSRMRGEKSLGQLLGITGYAT
                     "
     gene            674..1264
                     /locus_tag="VHV99_26735"
     CDS             674..1264
                     /locus_tag="VHV99_26735"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX3639015.1"
                     /translation="MEIDFDAITEYQRYKLMASLIVPRPIALVTTLGADGTINAAPFS
                     MFNMLGEEPPIVMISINRVGDGTLKDTAVNIARTGEFVVHLADEAIAEKMHRCGERLP
                     PNVSELAEVGLTPVASTHVAPPRIAEAPVAFECTLWETLETTSRQIFIGRVHRLHARD
                     ELIDTGTWRVRLQHYFPVGRFGASDYVTTRDRFTLG"
     gene            complement(1266..2852)
                     /locus_tag="VHV99_26740"
     CDS             complement(1266..2852)
                     /locus_tag="VHV99_26740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HEX3639016.1"
                     /translation="MHDTRLARSNFGMVTSPHALASEAGRDVLRAGGNAIEAAIAIGA
                     ALCVTYPHFTGLGGDAFWVIGDRQGMLRTLSGIGQAAQQLPAFSDAIPLRGAGSALTT
                     AATVDTWHQAYQISKDAWGGAQSWSSLFDRALEYASDGFPVTPSQHFWQRMRADELHA
                     LPGFAAAFAPQGRTPAIAERFVQPALAKSIERIARLGAREFYEGELAGRIARGLREAG
                     SPLTSSDLARTRARDEAPLRVAYRGGELVSMRPPTQGVTTLQIMGILARFDIAQIAEG
                     SADYYHVLVEAVKCAFVDRDRFVSDPEFNAVPVDDMLSPAALDAHARSINPRAARAWP
                     HVFRPGDTVFIGATDSEGRSVSVLQTLYFDWGSGVVAGDTGILWHNRGASFSTNAMHH
                     NVVQPGKRPFHTLNPGMYLKDGRPHLLFGTQGADGQPQTLAAILTRLIDYGMDPLTAL
                     ARPRFLLGKTFSDTRDALKLEEDAGAEVFAELAARGHEVSVMPAQSPLAGHPGVIRLE
                     RNGAASGAHDPRSDGRALGV"
     gene            3203..>4780
                     /gene="atzF"
                     /locus_tag="VHV99_26745"
     CDS             3203..>4780
                     /gene="atzF"
                     /locus_tag="VHV99_26745"
                     /EC_number="3.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004039 - allophanate hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase"
                     /protein_id="HEX3639017.1"
                     /translation="MPTASPLKVRSLLAAYAAKRLTPTEVVTSALARIVDIDRPEVWI
                     LRVPAADVLARANALEALYADQGSAVFERMPLFGVPFAVKDNIDVAGLPTTAACEPFS
                     YTPDTSAFAVQRLIDAGAILIGKTNLDQFATGLVGTRSPYGAVRNTEFPQRLSGGSSS
                     GSAVAVAAGCVAFSLGTDTAGSGRVPAGFNGLVGLKPSLGLVSKRGVVPACRSLDTIS
                     VFAHDVDDAWHVFSEIACFDPLDGYSRKVPGLGLLRGAPRIGVPDHVEFYGDAAANQA
                     FRAALDTLSTQLHLGPTPIDFKPLKQVSALLYDGPWVAERRAALGAFFETHHEAINPV
                     VATVIGKADRYSAADAFNGQYALADLKRHAEQLFETIDILIVPTTPTHPLIADVQANP
                     VELNSQLGYYTNFVNLLDLCALAVPCQRRHDGLPAGVTLIAPSGADRRLAELGARIQA
                     LFANATEAPNESETAAVTGSVSVAEKKARPAANTIKPLPFQEPTVALAVVGAHLRGQP
                     LNWQLQEAGARFIDATHT"
BASE COUNT          849 a         1553 c         1541 g          837 t
ORIGIN      
        1 tatcagtcga tcttcgacgg cgtgctgaac caccgtttga cgcccggcat gaagctgccg
       61 gagcccgagt tgtgccagct gttcggcgtg ggtcgtacgg tcgcgcggcg ggtgctggaa
      121 aagctcgcct acgacggcat cgtcgtgctg cggccgaaca aaggcgcggt gatcgccgag
      181 ccgacgctgg aggaaacgcg cgagatcttc gaagcccggc gctccgtcga acgcatgctg
      241 gtcgagttgg ccgtgcagcg tgcgagtgcg aaggacatcc tggccttgcg ccagcagttg
      301 gcgagcgagc acgaggcgat gcatcgcttc gaccagcctt cgtgggtcac gctcgccagt
      361 ggttttcata tgcgcatcgc cgcgctcgcg ggcaactcca ttctgcaaaa ctatctgaag
      421 gaactggttt cgcgctgctc gctcattgtc ggcgtgtatg aaccgccggg gcatgcgccg
      481 tgcgagcacg cggagcatgc ggcgattgtc gattgcatcg agacacgcga tgccgcgggc
      541 gcaatcgccc atatggaagc gcatcttcgc gatctcgagg agcggatcga aacgtcgcgg
      601 atgcgcggcg agaaaagcct cggccagttg ctcggcatca ccggctacgc cacttagacc
      661 caggccttca cacatggaaa tcgacttcga cgcaatcacc gaataccagc gctacaaact
      721 gatggcgagc ctgatcgtgc cgcgcccgat cgcgctcgtc accacgctcg gtgcggacgg
      781 cacgatcaat gccgcgccgt tttcgatgtt caacatgctc ggagaagaac cgccgatcgt
      841 catgatcagt atcaatcgcg tcggcgatgg cacgctcaag gacacggcgg tgaacatcgc
      901 gcgaactggt gagtttgtcg tgcatctcgc cgacgaggcg atcgcggaga aaatgcatcg
      961 ttgcggtgag cggcttccac cgaatgtgag cgaactcgcc gaggtcggcc tgacgccggt
     1021 ggcgagtacg catgttgcgc cgccgcgtat tgccgaagcg ccggtggcgt tcgaatgcac
     1081 gttatgggag acgctggaga cgaccagccg gcagattttc atcggccgcg tgcatcggct
     1141 gcatgctcgc gatgagttga tcgacaccgg gacctggcgc gtacggctgc agcattactt
     1201 tccggtggga cgcttcggcg ccagcgacta tgtcacgaca cgcgaccggt ttacgctcgg
     1261 ctgactcaaa cgccaagcgc ccggccgtcg ctgcgcggat cgtgagcgcc tgaggccgcg
     1321 ccgttccgct cgaggcggat cacgcccgga tggcccgcca atggactctg cgccggcata
     1381 acgctcacct catgcccgcg tgcggcgagt tcggcgaaca cctcggcgcc cgcgtcttct
     1441 tcgagcttca acgcatcacg cgtatcggag aaggtcttgc caagcagaaa gcgcggtcgg
     1501 gcgagggcag tgagcgggtc catcccgtag tcgatcaggc gcgtgagaat cgcggccagc
     1561 gtttgcggct gaccgtcggc gccttgcgtg ccgaacagca ggtgtggccg gccgtccttc
     1621 agatacatgc cggggttgag cgtatggaaa ggccgcttgc ccggttgcac gacgttgtga
     1681 tgcatggcgt tcgtgctgaa cgacgcgccg cggttgtgcc agagaatccc cgtatcgccc
     1741 gccaccacgc cgctccccca gtcgaagtaa agcgtttgca gcacgctgac gctgcggcct
     1801 tcgctatccg ttgcgccgat aaacaccgtg tcgcccgggc gaaacacgtg gggccatgcg
     1861 cgcgccgcac gcggattgat ggagcgtgcg tgtgcatcga gcgcggcggg cgacagcatg
     1921 tcgtcgaccg gcacggcgtt gaactcagga tcggacacga agcggtcgcg gtccacgaac
     1981 gcgcatttca cggcttccac cagcacgtgg tagtaatcgg cgctgccctc ggcgatctgc
     2041 gcgatatcga aacgagcgag gatgcccatg atctgcagtg tcgtcacacc ttgggtgggc
     2101 ggccgcatgc tgaccagttc gccgccacga taagcgacac gcaacggcgc ttcatcgcgc
     2161 gctcgggttc gcgccagatc gctcgacgtc aacggtgagc cggcctcgcg caaaccgcgt
     2221 gcgatccgcc cggctagctc gccctcataa aattcgcgcg cgcccaagcg tgcaatccgc
     2281 tcgatgcttt tcgccagcgc gggttgcacg aaacgttcgg cgatggcggg agtgcggccc
     2341 tgcggtgcga aagccgccgc aaaaccgggc agagcgtgca gttcgtccgc ccgcatgcgt
     2401 tgccagaaat gctgcgaggg cgtgacggga aacccatcgg atgcatattc gagcgcacgg
     2461 tcgaacaacg acgaccacga ttgcgcgccg ccccatgcgt ccttgctgat ctgataggcc
     2521 tgatgccatg tatcgacggt cgcggccgtg gtgagtgccg agccagcacc gcgcaacgga
     2581 atcgcgtcgc tgaacgcggg cagctgctgc gcggcctggc cgatgcccga cagcgtgcgc
     2641 aacatccctt gccggtcacc gatcacccag aacgcgtcac cgcccagtcc ggtgaaatgc
     2701 ggatacgtca cgcagagcgc tgcgccgatc gcgatcgcgg cttcgatcgc attgccgccg
     2761 gcacgcaata cgtcgcggcc cgcctcgctc gccagcgcat gagggctggt gaccatgccg
     2821 aagttcgaac gggcgagtcg ggtgtcgtgc atcgatactc cgcaatgagg aatgagctgt
     2881 gatcgaactc gtcggagcag cgagtataag ttcgcgcgcg gcgccgaaaa aaatcgatgc
     2941 gtaagggcat cgtgcgatcg cggcgcgccc tgtgcgccag gcgcgaatgc ggggcgcgtg
     3001 agccccgtgt tggggagcgt tgcaccatga cgcattgatc gaacggtgcc ggctcggccc
     3061 ggcccgagcg ctgggaaaat cgtcgcaaag ccaattctgg taaggctggc agcgcgctcg
     3121 ggcaagctcg caacgcactt ggcatagctc ctgctaagtt gagtccaaac ttgtattcaa
     3181 gattgaactc aaggaaaaac cagtgccaac tgcttcgccc cttaaggtcc gtagtctcct
     3241 cgcggcttat gctgccaaac gtcttacgcc cactgaagtc gtgacgtcgg cgcttgctcg
     3301 catcgtcgac atcgaccggc cggaagtctg gatcttgcgt gttcccgccg cagacgtgct
     3361 ggcccgcgct aacgcactcg aagcgcttta cgcagaccaa ggcagcgccg tgttcgaacg
     3421 gatgccgctg ttcggcgtgc cgttcgcggt caaagacaat atcgacgtcg ccggcctgcc
     3481 gaccaccgcc gcgtgcgagc ctttcagcta tacgccggac acttcggcat tcgctgtgca
     3541 acgcctcatc gatgccggcg cgatcctgat cggcaagacc aatctcgacc agttcgcgac
     3601 cggcctcgtc ggcacgcgtt ctccatatgg cgcggtgcgg aacaccgagt ttccccagcg
     3661 cctgtcgggc ggctcgagtt ccgggtccgc ggttgccgtg gccgcgggtt gcgtcgcctt
     3721 ttcgctcggc accgatacgg ccggatccgg gcgcgtaccg gccggtttca acgggctggt
     3781 cggcctgaag ccgtcgctcg gactcgtcag caagcgcggt gtcgtgccgg cgtgccgcag
     3841 tctcgacacg atctcagtgt tcgcgcacga cgtcgacgat gcgtggcacg tgttcagtga
     3901 aatcgcgtgc ttcgatccgc tcgacggtta ctcgcgcaag gtaccgggtc tcggtctcct
     3961 gcgtggcgcg ccgcggatcg gtgtgccgga tcatgtcgag ttctacgggg acgccgcggc
     4021 gaatcaggct ttccgcgccg cgctcgatac gctctcgacc cagttgcatc tcggtcccac
     4081 gccgatcgac ttcaagccgc tcaagcaggt ctccgcgctg ctctacgacg gcccctgggt
     4141 ggcggaaagg cgcgccgcgc tcggtgcctt cttcgagacg catcacgaag cgatcaatcc
     4201 ggtcgtcgca acggtaatcg gcaaagcgga ccgctacagc gccgccgacg cattcaacgg
     4261 ccagtacgca cttgccgacc tgaagcggca cgccgaacag ctcttcgaga cgatcgacat
     4321 cctgatcgtc ccgaccacgc cgacgcatcc attgatcgcc gacgtgcagg ccaacccggt
     4381 cgagttgaac agccagcttg gctactacac gaatttcgtc aatctgctcg atctgtgtgc
     4441 gttggccgtg ccgtgccagc gccgtcatga cggtttgcca gccggcgtca cgctgatcgc
     4501 gccgagcggc gcggaccggc gcctcgccga gttgggcgcg cggatccagg cgctgttcgc
     4561 gaacgccacc gaggccccca acgagagcga gacggcggcc gtgactgggt ccgtgagcgt
     4621 ggccgaaaag aaagcgaggc ccgccgcaaa cacgatcaag ccactcccgt ttcaggagcc
     4681 gaccgtcgcg ctcgccgtgg tcggcgcgca tctgcgtggc cagccgctga actggcaact
     4741 tcaggaagcc ggcgcgcgtt ttatcgacgc gacccacacc
//
LOCUS       DATASH010001006         4566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1756731,
            whole genome shotgun sequence.
ACCESSION   DATASH010001006 DATASH010000000
VERSION     DATASH010001006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1756731"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="VHV99_26750"
     CDS             complement(<1..942)
                     /locus_tag="VHV99_26750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018435424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HEX3639018.1"
                     /translation="MRSIMHANRLHLLLPFALPAAADASTALHDIQSPALDRLIARAT
                     LVERVVGEDFQRTLPHERWVARQFGALPSGAAAADEAPLAPYMLRADGGDPGNATWAC
                     VQPVHVRIAHDHLVLIDPASLELSDEEASVLLAVARPLIEELGVRIEAPRPARWYLSG
                     DGFGTLAGASPLRASGRNIEIWLPHEAHSGERSRAWMKLQNEVQMAWFEHPVNEAREA
                     RGLPAVNSIWFHAQGAVQPVKSPFAQVYSEAAATRGLALTAGVAAAAPPASFAALRAA
                     SAAGSGLRGASGGASGAPSSAASSGASDSASGAASSGA"
     gene            1062..1307
                     /locus_tag="VHV99_26755"
     CDS             1062..1307
                     /locus_tag="VHV99_26755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639019.1"
                     /translation="MPRELKTEQELLGLVVKALDANPATAGWIPTGFHETVEDEEGCN
                     WDITHLHRDRKDADVRGVANSEAAGIINDLRRHYNLR"
     gene            1706..2959
                     /locus_tag="VHV99_26760"
     CDS             1706..2959
                     /locus_tag="VHV99_26760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004532144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEX3639020.1"
                     /translation="MKFPYEWQIGWRYTRAGKRTTGNGFISFIALVSMSGIALGVAAL
                     IVVLSVMNGFQKEVRDRMLSVLAHVEIFSPTGSMPDWQLTAKEARQNKEVIGAAPYVD
                     AQALLTRQDAVSGVALRGVEPSLEPEVSDIGKEMKGGKLTDLVPGDFGIVLGADLATN
                     LGVTVNDKITLVAPEGTITPAGMLPRLKQFTVVGIFESGHYEYDSTLALINIKDAQAL
                     FRLPAPTGVRLRLTDMQRAPEVAHQLARSLSGDLYIRDWTQQNKTWFSAVQIEKRMMF
                     IILTLIIAVAAFNLVSSLVMTVTNKQADIAILRTLGAQPGSIMKIFVVQGVTIGFIGT
                     ATGVALGCLIAWSIPWLVPMIEHLLGVQFLPPSVYFISELPSELVPADVVRIGIIAFL
                     MSALATLYPSWRGAKVRPAEALRYE"
     gene            2952..3695
                     /gene="lolD"
                     /locus_tag="VHV99_26765"
     CDS             2952..3695
                     /gene="lolD"
                     /locus_tag="VHV99_26765"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HEX3639021.1"
                     /translation="MNDRSASLSMASQDSTLHPYVLEATGISKSFVQGGLNVQVLNNA
                     QLNVRRGEKLAIVGASGSGKSTLLHVLGGLDDPSAGHVSVLGKPFTKLSERERNDLRN
                     RALGFVYQFHHLLPEFTALDNVAMPLRIRRMTTEAARHEAFAVLERVGMGHRAKHRPG
                     ELSGGERQRVAIARALVTKPACVLADEPTGNLDGGTADTVFNLMLELSQTLDTSFVIV
                     THDPELAGRCDRIMRLRDGILHEEPSVPV"
     gene            3743..4531
                     /locus_tag="VHV99_26770"
     CDS             3743..4531
                     /locus_tag="VHV99_26770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX3639022.1"
                     /translation="MWIDTHCHLDASEFNADRDEVAHAARQAGVARIVIPAIGRQNFT
                     TVRELAHRIDGGAYALGIHPLFTPGAQERDLDLLRMEIEASLDDPLFVGIGEIGLDYF
                     VEGLDDARQQFFYNGQLQLAREFDLPVICHVRKSQDQVIKGLRRHQIHHGIAHAFNGS
                     FQQAQAYIDQGMHLGFGGNLTFERARQIRRLAEQLPFDALVVETDAPDIAPSWIYKQR
                     NSPEQIPAIGAGLAQLRGMSAGEAALGTTANALAALPRLAFSSA"
BASE COUNT          822 a         1470 c         1503 g          771 t
ORIGIN      
        1 agcgccactg gaggcggcac ccgaggcact atccgaagcg ccactggagg cggcactgga
       61 aggggcgccc gaagcaccac ccgaagcgcc acgcaaaccg ctgcctgcag cgctagcagc
      121 ccgcagcgcc gcaaacgacg ccggcggcgc agccgcagcg acgcccgcgg tcagggcgag
      181 gccgcgcgtc gccgccgcct cggagtacac ctgcgcgaac gggctcttta caggctgcac
      241 cgcgccctgg gcgtgaaacc agatcgagtt gactgccggc aggccacgcg cctcgcgcgc
      301 ttcattgacc ggatgctcga accacgccat ctgcacttcg ttctgcagct tcatccaggc
      361 gcgtgagcgc tcgccggaat gcgcctcgtg cggcagccat atttcgatgt tgcggccgct
      421 tgcgcgcaac ggcgacgcgc cggccagcgt gccgaagccg tcgcccgaca agtaccagcg
      481 tgccggcctc ggcgcttcga tgcgcacgcc gagttcctcg atcaacggcc gggcgaccgc
      541 cagcagcacg ctggcttcct catcggatag ttcgagcgag gccggatcaa tcagcaccag
      601 atggtcgtgc gcgatgcgca catggaccgg ttgcacgcag gcccacgtgg cgttgcccgg
      661 gtcgccgccg tcggcgcgca gcatgtacgg ggcaagcggc gcttcatcgg ccgccgccgc
      721 gccgcttggc aacgcgccga actggcgtgc cacccagcgc tcgtgcggca gcgtgcgctg
      781 aaaatcttcg ccgaccactc gttcgaccag cgtcgcgcgg gcgatcagcc ggtcgagggc
      841 cggactctgg atatcgtgca gggcggtgga agcgtccgct gcggcgggca gcgcgaaggg
      901 caggagaagg tgaagacggt tggcgtgcat gatgctgcgc attgtagggc aaagagtgcg
      961 agtattcaga ccatatcggc ggatccgggc agcgcaccgg ggccccgccg gtggcagcag
     1021 ccgcgcgcgg cggcaccgcg cccatgacga gggagaccga catgccgcgt gaactgaaaa
     1081 ccgagcagga actgcttgga ttggtggtca aggcgctcga cgccaatccg gccacggctg
     1141 gctggattcc aaccggattc cacgaaacag tcgaggacga agagggctgc aactgggata
     1201 tcacccacct gcatcgcgac cgcaaagacg ccgatgtgcg cggcgttgcg aacagcgagg
     1261 cggccgggat catcaacgat ctgcgccgcc attacaacct gcgttagcag ccacatccgc
     1321 ccaggatggc aggtgggccg gcttgcgccg ccgctccggc gttcagagcc ggcagcagac
     1381 gctggcgccc gccgtgagcc ctgcgcgcaa ggctttgcgc gggggcgcgg cattgtatgg
     1441 caaacttcgc gcttgattga cggcggcggc aatgacggcg ggtggcttcg gtggcgtgga
     1501 gtttgctgcc tacgccgtga gacacccgaa ggcaccggaa cgtcataccg gaaccatccg
     1561 ggttgccgcg acaccaatga tcacgacgct cgggccgcat gacaggcgcg acggcagcaa
     1621 gttgccgctg tgatgcgcag taaacgcggc caaggcgggc caaggcaggc ccaagagcag
     1681 acgaagaacg cagaaaagga ttcgcttgaa atttccctac gaatggcaga ttggctggcg
     1741 ctacacccgc gccggcaaac gcacgaccgg taacggcttc atttccttca tcgcgctggt
     1801 ctcgatgtcg ggcattgcgc tcggcgtcgc ggcgttgatc gtcgtgttgt ccgtgatgaa
     1861 cggtttccag aaagaggtgc gcgaccggat gctgtcggtg ctggcacacg tcgaaatctt
     1921 ttcgccgacc ggctcgatgc ccgactggca attgaccgcc aaggaagccc gccagaacaa
     1981 ggaagtgatc ggcgcggcgc cgtatgtcga cgcgcaggcg ctgctcacgc gccaggacgc
     2041 cgtgagcggc gtggcgctgc gcggcgtcga accgtcgctc gagcctgaag tgtccgacat
     2101 cggtaaggaa atgaagggcg gcaagctgac cgacctcgtg ccgggcgact tcggcatcgt
     2161 gctgggcgcg gacctcgcca ccaatctcgg cgtgacggtc aatgacaaga tcacgctggt
     2221 cgcgccggaa ggcaccatca cgcccgccgg catgttgccg cggctgaaac agttcacggt
     2281 ggtgggcatc ttcgagtcgg ggcattacga atacgacagc acgctcgcgt tgatcaacat
     2341 caaggacgcg caggcgctgt tccggctacc cgcgccgacc ggcgtgcggc tgcgcctgac
     2401 cgacatgcag cgggcgccgg aggtcgcgca tcagctggcg cgcagcttgt ccggcgattt
     2461 gtacatccgg gactggactc agcagaacaa gacctggttc tcggcggtgc agatcgaaaa
     2521 acgcatgatg ttcatcatcc tcacgctgat catcgcggtg gcggcgttca atctggtgtc
     2581 gtcgctggtg atgacggtga ccaacaagca ggctgacatc gcgattctgc gcacgctcgg
     2641 ggcacagccc ggctcgatca tgaagatctt cgtggtgcag ggcgtgacga tcggctttat
     2701 cggcacggcg accggcgtcg cgctgggctg cctgattgcc tggagcattc cgtggctcgt
     2761 gccgatgatc gagcatctgc tcggcgtgca gttcctgccg ccgtccgtgt atttcatcag
     2821 cgaattgccg tccgaactgg tgcccgccga cgtggtgcgg atcggcatca tcgcgttcct
     2881 gatgtcggcg ctggcgaccc tttatccgag ctggcgtggt gcgaaagtcc gtcctgcgga
     2941 ggcactgcgc tatgaatgac cgttccgcta gcctctccat ggcctctcaa gattccacgc
     3001 tgcatcccta tgtgctggaa gcaaccggca tttcgaagtc gttcgtgcag ggcggcctca
     3061 acgtgcaggt gctcaacaac gcgcaactga acgtgcggcg cggcgaaaag ctggcgattg
     3121 tcggcgcgtc gggttccggc aagagcacgc tgctgcatgt gctcggcggc ctcgacgatc
     3181 cgagcgcggg ccatgtctct gtgctgggca agcctttcac gaagctctcc gagcgcgaac
     3241 gtaacgacct gcgcaatcgc gcactcggtt tcgtgtacca gttccaccac ctgctgccgg
     3301 aatttaccgc gctcgacaac gttgcgatgc cgctgcggat tcgccgcatg acgaccgaag
     3361 ccgcgcggca tgaggcgttc gcggtgctcg agcgcgtcgg catggggcat cgggccaagc
     3421 atcgtccggg tgaactgtcg ggcggcgagc gccagcgtgt ggcaatcgcg cgggcgctgg
     3481 tgacgaagcc cgcttgcgta ctcgccgacg aacccaccgg caatctggac ggcggtaccg
     3541 ccgacacggt cttcaacctg atgctcgaat tgtcgcaaac gctcgacacc agcttcgtga
     3601 tcgtgactca cgacccggaa ctggccggac gttgcgaccg gatcatgcgc ttgcgcgacg
     3661 gcatattgca cgaagagccg tccgtgccgg tttaatcatc gcgcggaacg cgagcgatgc
     3721 tctgccgcca acgaggcacg ccatgtggat cgatacgcat tgccatcttg acgcgtcgga
     3781 attcaacgcc gaccgcgatg aggtcgccca tgcggcgcgt caggcgggcg tcgcgcgtat
     3841 cgtgattccc gcgatcggcc gtcagaactt cacgacggtg cgcgaactgg cgcaccgcat
     3901 cgacggcggc gcgtacgcgc tcggcatcca tccgcttttc acgccaggcg cgcaggagcg
     3961 cgatctcgac ctgctgcgca tggaaatcga ggcgagtctc gatgatccgc tgttcgtcgg
     4021 catcggcgaa atcggcctcg attatttcgt cgaaggtctc gacgatgcgc gccagcagtt
     4081 cttctacaac ggtcaacttc aactggcgcg cgaattcgat ctgccggtga tttgccacgt
     4141 gcgcaaatcg caggatcagg tgatcaaagg actgcggcgg catcagattc atcatggcat
     4201 cgcccatgct ttcaacggta gttttcaaca ggcccaagcg tatatcgatc agggcatgca
     4261 tctcggattc ggtggcaatc tgaccttcga gcgggcgcgg caaatccgcc gcctggccga
     4321 acaattgccg ttcgacgcgc tggtcgtcga gaccgacgcg cccgatatcg ccccttcctg
     4381 gatctacaag cagcgcaact cgccggagca gattccggcc atcggcgcgg gtctcgcgca
     4441 actgcgcggc atgagcgccg gcgaagccgc cctaggcaca acggctaacg cgctcgccgc
     4501 gctgccgcgg ctcgcatttt cctctgcata atcgaatgcg ctggtcttag tggttcgcgc
     4561 cgggcg
//
LOCUS       DATASH010001007        11584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2744386,
            whole genome shotgun sequence.
ACCESSION   DATASH010001007 DATASH010000000
VERSION     DATASH010001007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 11584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11584
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2744386"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1522
                     /locus_tag="VHV99_26775"
     CDS             <1..1522
                     /locus_tag="VHV99_26775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012363082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX3639023.1"
                     /translation="KQVVAVVGKEFSTDAWQEWYGEKTGTLINSGKYDGLAYEQAVDQ
                     IAADLKELGLGDKQITWRLRDWGVSRQRYWGTPIPIIHCPTCGDVPVPEKDLPVVLPE
                     DLVPDGTGNPLAKSEAFVNCTCPTCGGAAKRETDTMDTFVDSSWYFYRYASPDAKTMV
                     DERTDYWAPMDQYIGGIEHAILHLLYSRFWAKVMRDMGLVKFGEPARNLLTQGMVLNE
                     TYYRESEAGKKTWYNPADVTVSFDDKGRPVGAVLNSDGQPVVLGGVEKMSKSKNNGVD
                     PQLLIDQHGADTARLFVMFAAPPEQQLEWSGSGVEGASRFLRRVWNFGHANEAALRAG
                     GKLDAANFSDTDKVLRREIYSVLKQADFDYQRLQYNTVVSAAMKMLNALDGAKGAQPA
                     VLRETYSVMLRVLYPVVPHQTFQLWQELGYADEFGSLLDAAWPKVDEQALEQAEIELV
                     LQVNGKVRGALTVAKDATREAIEKLAAAHEAVEKFSEGKAPKKIVVVPGRLVNVVV"
     gene            1614..2165
                     /gene="lptE"
                     /locus_tag="VHV99_26780"
     CDS             1614..2165
                     /gene="lptE"
                     /locus_tag="VHV99_26780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEX3639024.1"
                     /translation="MTRRMLLTLACSVLMLSACGFQLRGQQDYAFKRLYISGGSAAAG
                     ARLARMVQGGSDTVVVTSVANADATLQITEGRGINTLTLNSLGVVEEYALNLSMTYTL
                     VGKDGTVLIPSSVIALNRAMTYSDQFAQAKAAESDLLFADMENDAIDQLTRRLSLVRS
                     MHPAPGQQVPSVAPRAPLPPPPL"
     gene            2250..3368
                     /gene="holA"
                     /locus_tag="VHV99_26785"
     CDS             2250..3368
                     /gene="holA"
                     /locus_tag="VHV99_26785"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEX3639025.1"
                     /translation="MQLRLDALEAHLAKGLAGLYVVYGDEHLLAQEASDRIRATARAA
                     GFTDRSVFTVERGFDWSSLLGASQSMSLFGDRQLVELRVPSGKPGKEGADALKALAAA
                     VNDDVLTMITLPRLDAATQKSAWFTALADAGVALKIDPVERAQLPNWVGQRLAAQGQR
                     VVAGEEGRRALAFIAERVEGNLLAAHQEIQKLGLLYPVGSLSFEQIQDAVLNVARYDV
                     FKLNEAMLAGDVGRLSRMLDGLRGEGEAAVLVLWAVVEEVRTLLRIKRGVGAGKPLAM
                     LVRENRVWGPRERLIGPALSRVTEGALEKALALAARLDRQVKGLSGGTPGNHRNDPPP
                     DPWDGLFELAMTVASPKAAPVPPPRPRAGAVAPTRRPG"
     gene            3752..5023
                     /locus_tag="VHV99_26790"
     CDS             3752..5023
                     /locus_tag="VHV99_26790"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006413382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEX3639026.1"
                     /translation="MDIDQYMTDLGRRARHASRAMARASTAAKNAALAAVAHAIERDA
                     ALLKEANARDVARARDKGLDAAFIDRLTLSDKALKTMVEGLRQVAALADPIGEISNLK
                     YRPSGIQVGQMRVPLGVIGIIYESRPNVTIDAAALCLKSGNATILRGGSEALECNTAL
                     AKLIGEGLEAAGLPQDAVQVVATSDRAAVGKLITMTEYVDVIVPRGGKSLIERLMNEA
                     RVPMIKHLDGICHVYVDDRADLTKALTVCDNAKTHRYGTCNTMETLLVARGIAAEVLP
                     PLGKLYRDKEVELRVDAAARAVLAAAGVGPLVDATEEDWRTEYLAPVLAIKVVDDLDV
                     AIDHINTYSSKHTDAIVTEDHDRAMRFLREVDSASVMVNASTRFADGFEFGLGAEIGI
                     SNDKLHARGPVGLEGLTSLKYVVLGHGEGRQ"
     gene            5185..5601
                     /locus_tag="VHV99_26795"
     CDS             5185..5601
                     /locus_tag="VHV99_26795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017333441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEX3639027.1"
                     /translation="MLWVKTFHIVLVASWFAGLFYLPRIFVNLAMETEPAATARLLLM
                     ARKLYRFMTMTAVPALACGLWLWLVIGIGRGQGWMHAKLTVVVLLIVYHFYCGRLLAT
                     FERGENRRSHKWYRMFNELPVLGMLAAVALVVIKPF"
     gene            6002..6364
                     /locus_tag="VHV99_26800"
                     /pseudo
     CDS             6002..6364
                     /locus_tag="VHV99_26800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004535949.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
     gene            complement(6682..7527)
                     /locus_tag="VHV99_26805"
     CDS             complement(6682..7527)
                     /locus_tag="VHV99_26805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEX3639028.1"
                     /translation="MMLSQVEAMREQLRPSERKLADYVIEAPREVLDLSMTEVAARAG
                     VSQPTIARFCHALGFSGFREFKIRLAQGIAAEVPAVYRDVRPDEPAPGVAAKVLDRTI
                     GALIQVRNNLSSDSVAAAIALLSQARRIEFYGAGGSGIAALDVQHKFFRLGMPSVAYS
                     DPHTFLMSAALLGEGDVVVAISNTGRTRDIVDAVKSALAAGAKVIAITHGNSPLARLA
                     TVGLFANVDEDTDIFSPMTSRVSHLAIGDILAVGVALSRGPELVEKMAQAKEVITRRR
                     IEPGA"
     gene            7819..9723
                     /gene="edd"
                     /locus_tag="VHV99_26810"
     CDS             7819..9723
                     /gene="edd"
                     /locus_tag="VHV99_26810"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HEX3639029.1"
                     /translation="MVSPHSQLLKVTQRVVERSKPTREAYLARVHHAQGKFPARGALS
                     CANLAHGFAGLEGNDKFAIKAIREPNIGIVSSYNEMLSAHAPYKNYPDIIKEAARENG
                     GVAQFAGGVPAMCDGVTQGNAGMELSLFSREVIAMSTAVALTHNMFDAALCLGICDKI
                     VPGLLIGALQFGHLPTIFVPAGPMGSGLSNDDKAKTRQLFATGQCGRDALLEAEAAAY
                     HSHGTCTFYGTANSNQMLMEVMGLHLPSSAFVHPHTPLRDALTAQAARRVLDLTVDRG
                     NYMPIGHVIDEKAIVNGIVALLATGGSTNHTLHLVAIARAAGIVIDWDDFDTLSQAVP
                     LLAKIYPNGKADVNHFHAAGGVAFLVRNLLEGGLLHEDVNTVAGKGLHHYTEEPKLID
                     GKLQWVPGAQTSEDTAVLRGIKEPFQPDGGLRLMQGKLGRGVIKISAVAAQHRKVKAP
                     AIVFDSQEAVQAAFDKGELKRDFIAVVRFQGARANGMPELHRLTPLLGVLQDQGFHVA
                     LVTDGRMSGASGKVPAVIHLSPEALLQGPIGKVRTGDMLVIDADAGVLDIQIDAAEWA
                     ARPEAAPLHQAENEVGFGRELFGVFRAAAAPAEQGASVFGPMVGERAAHHGERAKAHT
                     KEHTSTTQAS"
     gene            9749..10390
                     /gene="eda"
                     /locus_tag="VHV99_26815"
     CDS             9749..10390
                     /gene="eda"
                     /locus_tag="VHV99_26815"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEX3639030.1"
                     /translation="MTAKTVSDIVRLGPVIPVLAFDSVEQGEHVSRALHAGGVKVLEI
                     TLRTAAGLGAIERASQLADDIVVGVGTITRPEHCAQAKKAGAQFGVSPGLTRDMHKAA
                     QDAGLPLLPGVMTPSDIITALELGYEIVKFFPAQQAGGVPMLQAFHGPFPDLKFCPTG
                     GITAETATHFLSLPNVVCVGGSWLTPKAALAAQNWEEVTRLARAASQLAAPAH"
     gene            10642..>11584
                     /locus_tag="VHV99_26820"
     CDS             10642..>11584
                     /locus_tag="VHV99_26820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015295 - solute:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate:H+ symporter"
                     /protein_id="HEX3639031.1"
                     /translation="MEAVHGSTLLVFAVIAIALLILLITRYKVYPFLVLIIVSLLLGL
                     ASGMPIGTIVKSFETGNGNTLGHIAIVVGLGTMLGKMMAESGGAERIATTLIDKFGEK
                     HIHWAMMVVAIIVGLPVFFEVGFVLLIPIAFNVAKRTNKSLLLVGLPMVAGLSVVHGL
                     IPPHPAAMLAVQAYHADIGKTIAYGLIVGVPTAIVAGPLFALLISRYIKLPESNPLAA
                     QFLGHADDAQNDAKQAAPKRDLPSFGITLFTILLPVILMLIGSWADLVFTPKTLANDL
                     LRFVGNSDVALLIAVLVSFWTFGASRGFNREQIQKFCG"
BASE COUNT         2188 a         3496 c         3733 g         2167 t
ORIGIN      
        1 caagcaggtg gtcgcggttg ttggcaagga gttttccacc gacgcctggc aggaatggta
       61 cggcgagaaa accggcacgc tgatcaacag cggcaagtac gacggcctcg cgtacgagca
      121 ggcggtcgac cagatcgccg ccgatctgaa ggaactcggc ctcggcgaca agcagatcac
      181 gtggcgtctg cgtgactggg gcgtgtcgcg ccagcgttac tggggcacgc cgattccgat
      241 catccattgc ccgacatgcg gcgacgtgcc ggtgccggaa aaagatctgc cggtcgtgct
      301 gccggaagac ctcgtgccgg acggcacggg caatccgctc gccaagtccg aggcgttcgt
      361 gaattgcacg tgcccgacct gcggcggcgc ggcgaagcgc gaaaccgaca cgatggacac
      421 cttcgtcgat tcgtcctggt acttctatcg ctatgcgtcg ccggatgcga agaccatggt
      481 cgacgagcgc accgattact gggcgccgat ggaccagtac atcggcggta tcgagcacgc
      541 gattctgcac ctcttgtatt cgcgcttctg ggccaaggtc atgcgtgaca tgggtctggt
      601 caagttcggc gaaccggcca ggaatctgct cacgcagggc atggtgctca acgaaacgta
      661 ctaccgcgaa agcgaagcgg gcaagaagac ctggtacaac ccggcagacg ttaccgtctc
      721 gttcgacgac aagggccgcc cggtcggcgc cgtgctgaat tcggacggcc agccggtggt
      781 gctcggcggc gtcgaaaaga tgtcgaagtc gaagaacaac ggcgtcgatc cgcaattgct
      841 gatcgatcaa cacggcgcgg ataccgcgcg tctgttcgtg atgttcgccg caccgcccga
      901 gcagcagctc gaatggtccg gttcgggcgt ggaaggcgcg agccgcttcc tgcgccgcgt
      961 gtggaatttc ggtcacgcga acgaagccgc gttgcgtgcc ggcggcaagc tcgacgccgc
     1021 gaatttcagc gacaccgaca aggtgctgcg tcgcgagatc tacagcgtgc tgaagcaggc
     1081 cgacttcgac taccagcggt tgcagtacaa cacggtggtg tcggcggcga tgaaaatgct
     1141 caacgcgctc gacggcgcga agggcgcaca gccggcggta ctgcgcgaga cgtatagcgt
     1201 gatgctgcgc gtgctgtatc cggtcgtgcc gcatcagacg ttccagttgt ggcaggaact
     1261 cggttacgcc gacgaattcg gctcgctgct cgacgctgcg tggccgaagg tcgatgaaca
     1321 ggcgctggaa caggccgaaa tcgaactcgt gctgcaggtg aacggcaagg tccgcggcgc
     1381 gctgacggta gcgaaagatg cgacgcgtga agcgattgaa aagctcgccg ccgctcatga
     1441 ggcggtcgag aagttcagcg aaggcaaggc accgaagaag atcgtcgtgg tgccggggcg
     1501 cctcgtgaac gtggtggttt gacagtccac cgaaacatga cagcagacaa gcgccgctgt
     1561 gcgcggcggc gcttgtctct cgctgcggaa tctactgcga accaggagcc aatgtgactc
     1621 gcagaatgtt attgacgctg gcgtgcagcg tgttgatgtt gtccgcctgc ggcttccagt
     1681 tgcgcggtca gcaggactac gcattcaaac gactctatat ctccggcggt tcggctgcgg
     1741 ccggcgcgcg gctcgcacgc atggtgcagg gcggcagcga cacggtggtg gtcacctccg
     1801 tcgcgaatgc cgatgcaacg ctgcagatca ccgagggtcg cggcatcaat acgctcacgc
     1861 tgaactcgct cggtgtcgtc gaagagtatg cactgaatct gtcgatgacc tacacgctgg
     1921 tcggcaagga cggcacggtg ctgattccgt cgagcgtgat cgcgctaaac cgcgcgatga
     1981 cctatagcga ccagtttgcg caagccaagg ccgctgaatc cgacctcctc ttcgcggaca
     2041 tggaaaacga cgcgatcgac cagcttacgc gccggttgtc gctggtgcgc tcgatgcatc
     2101 cggcgccggg tcagcaggtg ccgtcggtgg cgccgcgcgc gccgttgccg ccgccgccgc
     2161 tgtgagcgtc gcattgtgtc tttgtttcgc ttgctgcatt tgcccatgta ctcgctcatg
     2221 cagcccgtta acttcgccga tcggtagcca tgcaactgcg acttgacgcg ctcgaagcgc
     2281 acctcgcgaa gggactcgcc gggctgtacg tcgtgtacgg cgacgagcat ctgctcgcgc
     2341 aggaagcgag cgaccggatt cgtgcgacgg cgcgcgcggc cggttttacc gatcgttcgg
     2401 tgttcacggt tgagcgcggc ttcgactgga gttcgctgct tggcgcgagt caatcgatgt
     2461 cgctgttcgg cgaccgtcaa ctggtcgaat tgcgcgttcc ttcgggtaag cccggcaagg
     2521 agggcgcgga tgcgctgaag gcgctcgccg ccgccgtcaa tgacgatgtg ctgaccatga
     2581 tcacgttgcc gcggctcgat gcggctacgc aaaagtcggc gtggtttacc gcactggccg
     2641 atgccggtgt ggcgttgaaa atcgatccgg tcgagcgtgc gcagttgccg aattgggtgg
     2701 gtcaacggct tgccgcgcag ggccagcggg tcgttgccgg ggaagaaggg cggcgcgcgc
     2761 tggcgttcat cgccgagcgc gtggaaggca acctgctcgc tgcgcatcag gaaatccaaa
     2821 agctcgggct gctgtatccg gttggttcgt tgagctttga acagattcag gacgcggtgc
     2881 tgaacgtcgc gcgctatgac gttttcaagc tgaacgaagc gatgctggcg ggcgatgtcg
     2941 gtcggctctc gcgcatgctc gacggcttgc gtggcgaagg cgaagctgcg gtgctggtgc
     3001 tgtgggcagt ggtcgaagaa gtgcggacat tgttgcggat caagcgcggt gtcggggcgg
     3061 gcaagccgct cgcgatgctg gtgcgcgaga accgcgtgtg ggggccgcgt gagcggctga
     3121 tcgggccggc attgtcgcgc gtcactgaag gcgcgctgga gaaagcgctg gctctggcgg
     3181 cgcggctgga tcggcaggtt aaaggcttgt cgggcggcac gccgggcaat catcgcaacg
     3241 atccgccgcc cgatccgtgg gacggtctgt ttgaactggc catgacggta gcttcgccaa
     3301 aagcagcgcc cgtgccgccg ccacgacctc gggccggggc ggttgcgccg acacgcagac
     3361 cgggctgatc tggacgatcg aggcagttag agtggttgcc tgaagtggct gaagtgccag
     3421 aagtgccaga agtgccagaa gtgccagaag tggctggcca ggctgacgag gtcccgacgc
     3481 cgctaaagtg gtccacgtgg ctggtgaggc tgacatgttg acgagtggct ggcgagcttc
     3541 tccaatcagc aggcatggag acacggtatg gtcggtgtcg gcgcaatatc aggccccgca
     3601 ccattaacag cggcggcgcc gcggatctca ccgcagccac tccagccgcc acgacaagca
     3661 aacctccgcc acccgactta caatctttcg attcttcgct aactttgcct gtccgctgca
     3721 cctcgatgtg cgcggcatca cgagaatcac catggatatc gaccagtaca tgaccgacct
     3781 cggccgccgc gcccgccacg cttcgcgtgc gatggcgcgg gcgtcgacgg cggcgaagaa
     3841 cgccgcgctc gccgcagtcg ctcacgcgat cgaacgcgat gccgcgctgc tgaaggaagc
     3901 caacgcgcgt gatgtagccc gtgcgcgcga caaaggtctc gacgccgctt tcatcgaccg
     3961 cctgacgctg tcggacaagg cgctgaagac gatggtcgaa ggcctgcggc aagtcgccgc
     4021 gctggccgat ccgatcggcg agatcagcaa tctgaagtac cggccgagcg gcattcaggt
     4081 cggccagatg cgagtgccgc tcggcgtgat cggcatcatc tacgaatcgc gtccgaacgt
     4141 gacgatcgac gccgcggcgc tttgcttgaa gtcgggcaac gcgacgattc tgcgtggcgg
     4201 ctctgaagcg ctggaatgca acacggcgct cgccaagctg atcggcgaag gactggaagc
     4261 cgccggcttg ccgcaggacg ccgtgcaagt ggtggccacc tcggaccgcg ccgcagtcgg
     4321 caagctgatc acgatgaccg aatatgtcga cgtgatcgtg ccgcgcggcg gcaagagcct
     4381 gatcgagcgg ttaatgaacg aggcgcgtgt gccgatgatc aagcacctcg acggcatctg
     4441 ccacgtatat gtcgacgatc gcgcggatct gaccaaggcg ctgaccgtct gcgacaacgc
     4501 gaagacacac cgctacggca cctgcaacac gatggaaacg ctgctggtcg cacgcggtat
     4561 cgcggctgaa gtgctgccgc ctttgggcaa gctgtatcgc gataaggaag tcgaattgcg
     4621 cgtggatgca gccgcccgcg cagtgttggc cgccgccggg gtcggcccgc tcgtcgatgc
     4681 taccgaagaa gattggcgca ccgaatatct cgcgcccgtg ctggcgatca aggtggtcga
     4741 cgatctcgac gtggcgatcg accacatcaa cacatatagc tcgaagcaca ccgatgcgat
     4801 cgtcaccgaa gaccacgacc gtgcgatgcg cttcctgcgc gaagtggatt cggcgagcgt
     4861 gatggtcaac gcctcgacgc gttttgcgga tggcttcgaa ttcggccttg gcgcggagat
     4921 cggcatctcg aacgacaagc tgcacgcgcg cgggccggtg gggctggaag ggctgacgtc
     4981 gctgaagtac gtcgtgcttg ggcatggcga aggccggcaa taagtcgcca tcggccgcgc
     5041 tttcatcgcg cacgccggaa taagggccgt cggctgtgcg tttaatcgcg aacgccgccg
     5101 ggaaatgcgg ccggctatct cttttcatta cgaacgcgcc gccacgagcg ctgatttcca
     5161 cgatacaagt cgcaaggact gttgatgctt tgggtaaaga cgtttcacat cgttctggtt
     5221 gcttcctggt ttgccggcct gttctatctg ccgcgcatct tcgtcaatct ggcgatggag
     5281 acggagcccg ctgccacggc gcggcttctg ctcatggcga ggaagctgta ccgcttcatg
     5341 acgatgaccg ctgtccctgc gctggcctgt gggctgtggt tgtggcttgt gatcggcatc
     5401 gggcgcggcc agggctggat gcacgcgaag ctgaccgtcg tcgtgctgct gatcgtctat
     5461 catttttatt gcggccggct gctggctacc tttgagcggg gcgagaaccg ccgctcgcat
     5521 aagtggtatc ggatgttcaa tgagctgcct gtgctgggga tgctggcggc ggtggcattg
     5581 gttgtgatca agccgtttta gcgctaaaga taggaactct cctatttact cttttttttc
     5641 ggttatcttt atccgcgtgc ggtattttta agcgcgcgga ttttcacctg atagccaact
     5701 ctttataagc cgacattctt taagaatcgt caatcccatt tcggcctttc ctgtgccgat
     5761 cctcgcccta tcaaacagtc gtcaccaaga ctgaggggct tcggaatttt ccgaatttga
     5821 actgggtgtc agccgcgttc ccacgcggct gttgtacaag tccagtcatc cgtgatcctt
     5881 gcctgtagtg gcttctcggc gtttgtgtgt cgcgtaacgc cctgtctcgc gtgggaataa
     5941 tccgattgcc gacagttttc gtttaagaac cgtcctatta gtcagcgaaa ttcctacttt
     6001 tatagccgat gaggcgaaca ccgtctcggt cgtttcacaa ggcaacgagc gccgtatcag
     6061 caacgtcgtg aacggcacag cggcgacgga tgccgtgaac ctggggcaga tgcagtcggc
     6121 catcggcgat accgcgcgtg ccgcattcag cggcgtcgcc gccgccaccg cgctgacgat
     6181 gattccggaa gtcgatccgg gcaagacgct ggcagtcggc gtcgccggag caaactacaa
     6241 gggctatcag gccgcggcga tcggcgcgtc agcgcggatc acggcgaatc tgaagatcaa
     6301 gatgggtgcg ggcatcagtg gaaacaatac gacggttggc gccggtgctt cgtatcagtg
     6361 gtaagtgtgt gacggtggtg aaaaggcgcg gcggttgagc cccgcctttc cgcgggccgc
     6421 aagtttgacc cggtagtaaa gaaaaaagga cgggctccct tcgaggagct cgtccttttt
     6481 tgcgcgtgcg gttgacgtgc cgaacagtgg atacgattgc gcggacacgt cgcccatctt
     6541 tcccgagcga cgcgttgccc tgctaccgct gctgtcgcta cgggaacgca tgtcactgca
     6601 aacgtctggt gtgcgaccaa ccgcaaaacg tcgggcgtgc gaccaaccgc aaaacgtccg
     6661 tgtgcgacca accgtaaaac atcacgcgcc cggctcgatc cttctgcgag taatcacttc
     6721 cttcgcctgc gccatcttct cgacgagctc aggcccccgg ctcaaagcca cacccactgc
     6781 gagaatatcc ccgatcgcca gatgtgaaac gcgcgaggtc atcggcgaaa agatatccgt
     6841 gtcttcgtcg acgttggcaa acaacccgac cgtagcaagc cgcgccaatg gcgaattgcc
     6901 atgcgtgatc gcaatcacct tcgcgcctgc tgccaacgcg gatttcaccg catcgacgat
     6961 atcgcgcgtg cgacccgtat tcgaaatcgc caccaccaca tcgccttcac ccagcagcgc
     7021 ggccgacatc agaaacgtat gcgggtccga atacgcgacg ctcggcatgc cgaggcgaaa
     7081 gaacttgtgc tgcacatcca gtgctgcgat gcccgaaccg cccgcaccgt agaactcaat
     7141 gcggcgcgct tgcgacagca acgcgatcgc cgccgccacg ctatccgatg acagattgtt
     7201 gcgcacctgg atcaaggcgc cgatggtccg atcaagcacc ttcgcggcga cgcccggcgc
     7261 cggctcatcc ggccgcacgt cgcggtagac ggcgggcact tccgccgcga tcccctgcgc
     7321 cagccggatc ttgaactcgc gaaagcccga aaagccgagt gcatgacaga accgcgcgat
     7381 ggtcggctga ctgacgccgg cgcgcgcggc cacttcggtc atcgacaaat cgaggacttc
     7441 gcgtggcgct tcaatcacgt aatcggccag cttgcgctcg gacgggcgca actgttcgcg
     7501 catcgcttcc acctgggaca gcatcatcgg aaaactcgca ctatgctgaa gaatgcgtgg
     7561 actatatctg attgctgaga aagctacaaa aactacatat ttgcagctag gtgtaatccc
     7621 tggtttaggc gtgtacatcg ctaaggcaaa gccagtaagg cgatgcggca tttcaaagat
     7681 gccgcagtgc aacaggagtc ctgattgcga tcgtgtagtt tttctactaa gattgcatcg
     7741 tcgactgatc cgacaccccc gcgataccta cctggcacag tgcgcgcacg cctccacgcc
     7801 agcgacgaag gagctccgat ggtttccccg cattcgcaac tgttgaaagt cacgcaacgc
     7861 gtggtcgagc gtagcaaacc cactcgcgag gcatatctgg cccgcgtcca tcacgctcaa
     7921 ggcaaattcc cggcgcgcgg tgcactctcc tgcgccaatc tggctcacgg ttttgccggc
     7981 ctcgaaggca acgacaagtt cgccatcaag gcgatccgcg agccgaacat cggcatcgta
     8041 tcgtcgtaca acgaaatgtt gtccgcgcac gcgccgtaca aaaactaccc cgacatcatc
     8101 aaagaggccg cgcgcgagaa cggtggcgtc gcgcaattcg cgggcggcgt gccggccatg
     8161 tgcgacggcg tcacgcaagg caacgcgggc atggagctgt cgctgttctc gcgcgaagtg
     8221 atcgcgatga gcacggcggt cgccctcacg cacaacatgt tcgacgcggc gctttgcctc
     8281 ggcatctgcg acaagatcgt gccgggcctc ttgatcggcg cgctgcagtt cggccatctg
     8341 ccgaccattt tcgtgccggc cggcccgatg ggcagcggcc tctcgaacga cgacaaagcc
     8401 aagacccgtc agctcttcgc caccggccag tgcggccgcg acgcattgct cgaagcggaa
     8461 gccgccgcgt atcacagcca tggcacctgc acgttctacg gcaccgccaa cagcaatcag
     8521 atgctgatgg aagtgatggg cctgcatctg ccgagttcgg ccttcgtgca tccgcatacg
     8581 ccgctgcgcg acgcgctgac cgcgcaggcc gcgcgccgcg tgctcgatct gacggtggac
     8641 cgcggcaact acatgccgat cggccacgtg atcgacgaga aagcgatcgt caacggcatt
     8701 gtcgcgttgc tggcgacggg cgggtcgacc aatcacacgc tgcacctggt cgcgattgcg
     8761 cgtgcagccg gcatcgtgat cgactgggac gacttcgaca cgctgtcgca agccgtgccg
     8821 ttgctcgcca agatttaccc gaacggcaag gccgatgtga accacttcca tgctgcaggc
     8881 ggcgtcgcat ttctggtccg caatctgctg gaaggcggct tgctccatga agacgtgaac
     8941 acggtcgccg gcaaagggct gcaccactac accgaagagc cgaagctgat cgacggcaag
     9001 ctgcaatggg tgcccggcgc gcaaacgagc gaagacacgg cggtgctgcg cggcatcaag
     9061 gaaccgttcc agccggacgg cggcttgcgt ctgatgcaag gcaagctcgg ccgcggcgtg
     9121 atcaagattt ccgcagtggc ggcgcagcat cgcaaggtga aggcgccggc catcgtgttc
     9181 gattcgcagg aagcggtgca ggccgccttc gacaagggcg aactgaagcg cgatttcatt
     9241 gctgttgtgc gctttcaggg cgcgcgggca aatggcatgc cagagctgca tcgtttgacg
     9301 ccgttgctcg gtgtgttgca ggatcaaggt ttccacgtcg cactggtcac ggacggccgc
     9361 atgtcgggcg cgtcgggcaa ggtgccggcg gtgattcacc tgtcgccgga agcattgctg
     9421 caaggcccga tcggcaaggt tcgcaccggc gacatgctgg tgatcgatgc ggatgccggc
     9481 gtgctggaca tccaaatcga tgcggccgaa tgggcggcac ggccggaggc agcaccgctg
     9541 catcaggcgg agaacgaagt cggcttcggc cgtgaactgt tcggcgtgtt ccgtgcggcg
     9601 gcggcgccgg cggagcaggg cgcctcggtg ttcggtccga tggtgggcga gcgcgcggcg
     9661 caccacggtg agcgggccaa agcccacacc aaagaacaca cgagcaccac tcaagccagc
     9721 taaacgctgc agaaaaagga gtctgactat gacggcgaaa acagtaagcg atatcgtgcg
     9781 cctcggcccg gtgattccgg tgcttgcgtt cgactcggtc gagcagggtg aacacgtgtc
     9841 gcgcgcactg cacgcgggtg gcgtgaaggt gctcgaaatc acgctgcgca ccgcagcggg
     9901 tctgggcgca attgaacgcg cgagccagtt ggccgacgac atcgtggttg gcgtcggcac
     9961 gatcacgcgg cccgagcact gcgctcaggc gaaaaaagcg ggcgcgcagt tcggcgtctc
    10021 gccgggtctg acgagagaca tgcacaaggc ggcgcaggat gcgggcttgc cgctgctgcc
    10081 gggcgtgatg acgccgtccg acatcatcac cgcgctggaa ctcggctacg agatcgtgaa
    10141 gtttttcccg gcacagcagg ccggcggcgt gccgatgctg caagcttttc atggcccgtt
    10201 cccggacctg aagttctgcc cgaccggcgg tatcacggcc gagacggcga cgcatttcct
    10261 gtcgctgccg aacgtggtgt gcgtcggcgg ctcgtggctc acgccgaaag cggcgctggc
    10321 cgcgcaaaac tgggaagaag tcacgcgtct cgcgcgtgcg gccagccagt tggccgcgcc
    10381 ggcccactaa gttgaaacgg cacgctattt aaccgaccag tcgggtccga caaaggagcc
    10441 ccctcgatac agtcttttcg gctgtcgcgg cggggctctt ttttcgagac gtgtaccggt
    10501 ttagcgcgcc ttgagggacg acaaacagta gaattcagcg tccgtgcggt tggccgggtt
    10561 tcgaggtcgc cgcaggccgc aacgagacac ggtgcctgct gcgttgcgca cctaaaatca
    10621 ataatgcaaa ggaggagctc catggaagct gtccacggca gcacgctgtt ggtcttcgcg
    10681 gtgatcgcca tcgcgcttct gatcttgctg atcacccgct acaaggtcta tccgttcctc
    10741 gtcctgatca tcgtttcgct gctgctgggt ctcgcatccg gcatgccgat cggcactatc
    10801 gtcaaatcgt tcgaaaccgg caatggcaat acgcttggtc acattgcgat cgtggtcggg
    10861 ctcggcacga tgctcggcaa aatgatggcc gagtcgggcg gcgcggagcg catcgcgacc
    10921 acgctaatcg acaagttcgg cgagaaacac attcactggg cgatgatggt cgtggcgatc
    10981 atcgtcggct tgccggtgtt ttttgaagtc ggtttcgtgc tgctgattcc gatcgcgttc
    11041 aacgtcgcca aacgcaccaa caagtcgctg ctgctggtcg gcttgccgat ggtcgcgggt
    11101 ctgtccgtcg tgcacggttt gattccgccg cacccggccg cgatgctcgc ggtgcaggcc
    11161 tatcacgccg acatcggcaa gacgattgct tacgggctga tcgtgggcgt gccgacagcg
    11221 atcgtcgccg gtccgctgtt cgcgttgttg atcagccgtt acatcaagct gccggagagc
    11281 aatccgctgg ccgcgcaatt cctcggccac gctgacgacg cgcaaaatga cgccaaacag
    11341 gcggcaccta aacgcgatct gccgagcttc ggcatcacgc tgttcacgat cctgttgccg
    11401 gtgattctga tgctgatcgg cagttgggcc gacctcgtgt ttacgccgaa gaccctcgcg
    11461 aacgacctgc tgcgtttcgt cggcaattcg gacgtcgcgc tgttgatcgc cgtgctggtc
    11521 agtttctgga ccttcggcgc gagccgcggc ttcaatcgtg agcagatcca gaagttctgc
    11581 ggcg
//
LOCUS       DATASH010001008         5286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6500493,
            whole genome shotgun sequence.
ACCESSION   DATASH010001008 DATASH010000000
VERSION     DATASH010001008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5286
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6500493"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..847
                     /locus_tag="VHV99_26825"
     CDS             2..847
                     /locus_tag="VHV99_26825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX3639032.1"
                     /translation="MQIVNGIGVGMLYFLLAVGLSIVFGLLRFVNFAHGAFYLLGAYF
                     CYQAMQWSMSFWTALVVVPIVVGALAWVVEKLILRHVYAQQHEFHILATVGLALVVQE
                     CAILIWGPLGDNVAVPDILNGVVIWGSFVYPKYRLFVIGLTAVLAALLWWVLEGTRLG
                     SAVRAGSESTEMVSLLGINVLRVFSLVFALGAATAALAGVLAAPICGVDPFMGIEALS
                     VAFVVVVVGGMGNFLGALVGGLLVGIVQSVMSTLWPEGARLMIYVAMAAVLLLRPNGL
                     LGRAA"
     gene            844..1911
                     /locus_tag="VHV99_26830"
     CDS             844..1911
                     /locus_tag="VHV99_26830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX3639033.1"
                     /translation="MIDTSKPGVQAASSRPAQRSPKHALHRYRFLLLALLVVCVLPLT
                     LRSGSLATEVLVFALAALGCNLLLGHTGLLSFGQGIFFGLGSYCAGLVLTRTALPIPV
                     ALLAAIVTGAAAAALVGWFSIRQRGTYFVMLTLAFGQLFYFLAYTTPDLTGGDNGLLD
                     IPRPALGLVGKALVPLTSPWQYYGFVAVLFLVVFWLLMRVSHSVFGRTLLAIRDNEAR
                     AAAVGYDVKRFKLMAFVISGAVTGLAGALHALMTGIAPLSNIDYHTSEMILVMTVIGG
                     TGNLFASVLGAAFYVLFADWLSTLWPRWLLLLGIVLIAVSLFMQRGLWGLGERIWQAL
                     RRNPSADADNDAGNHAARERV"
     gene            1908..2675
                     /locus_tag="VHV99_26835"
     CDS             1908..2675
                     /locus_tag="VHV99_26835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006497938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3639034.1"
                     /translation="MSEAILQASGVVKRYGKFTALSQVNLRVMPRTVHSVIGPNGAGK
                     TTLFHVLTGTLPITEGKIIFDGHDVTHEPDHKRVRRGVARSFQVTSLFANLSVRENLR
                     LAAQGVDAVRALNAWTPPHGALTHADIVDSVLERLALQRFSEVPAGALSHGQQRRLEV
                     GMALAARPKAIFLDEPTSGMGIDDLDDMKQLIRGLRDDYTVVLIEHNMGIVMDISDTI
                     TVMQQGRVLVEGRPDDIRGDERARSAYLGNMITGGRA"
     gene            2672..3373
                     /locus_tag="VHV99_26840"
     CDS             2672..3373
                     /locus_tag="VHV99_26840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3639035.1"
                     /translation="MILDVQQIHGFYGKSHILQGVSLQINDGETVTLLGRNGAGKSTT
                     LKAIAGVVTPQRGTVTFNGVAISGMPPHKIASRGVCFVPEHRGVFRLLTVEENLRLGA
                     RKDSPWQLDDIYRIFPRLKERRANGGAQLSGGEQQMLAIGRALMNHPRLLMLDEPVEG
                     LAPVIVEEIVAQLKLIREAGVAILLVEQNLEVCTQLADRHYIIEQGVIVYEGDNATFA
                     ADDSIKDRYLGVGVV"
     gene            3407..4687
                     /locus_tag="VHV99_26845"
     CDS             3407..4687
                     /locus_tag="VHV99_26845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HEX3639036.1"
                     /translation="MQVHESTTVRTLADLRIDGTRLWDSLMQLAQIGATDKGGVCRLA
                     LTELDRKARDLFIAWAKEIGCSVRIDAIGNIFARRAGLRDDLPPVMTGSHIDTQPTGG
                     KFDGNYGVLAGLEVLRTLVDANVRTLAPLEVAVWTNEEGSRFVPVMMGSGVFAGAFTL
                     EHALEQRDREGISVRDALASIGYAGENGTPHAVGAYFEAHIEQGPVLEAHDTTIGVVQ
                     GALGQRWYDVTVHGMEAHAGPTPMELRRDALLVAADLIHAVNRIALDHAPHGRGTVGW
                     LGVHPNSRNVIPGRVTLTVDLRAADDASLTEMDAALRAACAAAGKQAGITVDVEQVVY
                     FPPQPFAADLVAAIQEGADTLGFSSMDVISGAGHDAVYLARVAPAAMIFVPCKDGISH
                     NEIEDARADHLEAGCNVLLQAMLNAAQTAGSTQV"
     gene            4684..>5286
                     /locus_tag="VHV99_26850"
     CDS             4684..>5286
                     /locus_tag="VHV99_26850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HEX3639037.1"
                     /translation="MKILIARMNHETNTFSPVATPLAAFGRNGPSYGEDALNENKGMQ
                     TAMAAFIDAAEREHAAIVTPISASANPSGPVEAAAYDAICDAIVAAAAGCDAVMLDLH
                     GAMVAENSNDGEGDLLERLRAALPNAPIAVALDLHGNVTQKMVDNADVIVSFKTYPHV
                     DMYETGAHAARLMFDLVHGKAKPVIAWRQPPLLTHTLRSAT"
BASE COUNT          902 a         1587 c         1729 g         1068 t
ORIGIN      
        1 cctgcagatc gtcaacggca tcggagtggg gatgctgtat ttcctgctcg cggtcggctt
       61 atcgatcgta ttcggcctgc tgcgtttcgt gaacttcgcg cacggcgcgt tctatctgct
      121 tggcgcctac ttctgctatc aggcgatgca gtggtcgatg agtttctgga ccgcgctcgt
      181 cgtcgtgccg atcgtggtgg gcgcgctggc gtgggtcgtc gagaaactga ttctgcggca
      241 cgtctacgcg cagcagcatg agtttcatat tctcgcgacc gtcggccttg cactcgtcgt
      301 gcaggaatgc gcgattctga tctggggccc gctcggcgat aacgtcgcgg tccccgacat
      361 attgaacggc gtggttatct ggggcagctt cgtttatccg aagtaccggc tgttcgtgat
      421 cggtctcacg gcggtgctgg ccgcgctctt gtggtgggtg ctggaaggca cgcgcctcgg
      481 cagcgcggtg cgcgcgggca gtgaatcgac cgagatggtg tcgctgctcg gcatcaacgt
      541 attgcgcgtt ttcagcctcg tgttcgcgct cggcgccgcg actgccgcgc tggcgggcgt
      601 gctcgccgcg ccgatttgcg gcgtcgatcc gttcatgggc atcgaggcgc tcagtgtcgc
      661 gttcgttgtg gtggtggtcg gcggaatggg caattttctg ggcgcgctgg tcggcgggct
      721 gctggtcggc atcgtgcaga gcgtaatgag tacgttgtgg ccggaaggcg cgcggctgat
      781 gatctatgtc gcgatggccg cggtgctgtt gctccggcca aacggcttgc tcgggagagc
      841 ggcatgatcg atacctcgaa acctggtgtg caagccgctt cttcccgacc ggctcaacgc
      901 agcccgaagc atgcgttgca tcgctatcgc ttcctgttgc ttgcgttgct ggtcgtgtgc
      961 gtgttgcccc tcacgctgcg ctccggatcg cttgctacgg aagtactggt gttcgcgctc
     1021 gccgcactcg gttgcaatct gctgctcggc cacacgggcc tgctctcgtt cggacagggc
     1081 attttcttcg gactcggcag ctattgcgcg ggcctcgtcc tcaccagaac ggcgctgccg
     1141 atacccgtgg cgctgcttgc cgcgatcgtg accggcgcgg ctgccgctgc actggtcggc
     1201 tggttttcga ttcgtcagcg cggcacgtac ttcgtgatgt tgacgctcgc attcgggcaa
     1261 ctgttctact tcctcgctta caccacaccc gatctgaccg ggggtgacaa cggcttgctc
     1321 gacattccac gtccggcgct ggggttggtc ggcaaagcac tcgtgccgtt gacctcgccg
     1381 tggcagtact acggtttcgt cgccgtgctg ttcctcgtgg tgttctggtt gctgatgcgc
     1441 gtctcgcact cggtattcgg ccggacattg ctggcgattc gagacaatga ggcgcgcgcg
     1501 gccgcggtcg gctacgacgt caaacgcttc aagctcatgg cgttcgtgat ctccggcgcg
     1561 gtgacgggcc tcgctggcgc gttgcacgcg ctgatgaccg gtatcgcgcc actgtccaac
     1621 atcgattacc acacaagcga gatgatcctt gtgatgacgg tgatcggcgg tacgggcaat
     1681 ctgttcgcgt cggtgctggg tgcggcgttc tatgtgctgt tcgccgattg gctttccacg
     1741 ctatggccgc gctggctgtt gctgctcggc atcgtgctga tcgccgttag cctcttcatg
     1801 cagcgcgggc tatggggttt gggcgaacga atctggcaag cgctgcgacg caacccatcc
     1861 gccgatgctg acaacgatgc aggcaaccac gcagcccggg agcgcgtatg agcgaagcga
     1921 ttctgcaagc gagcggtgtg gtgaagcggt atggcaaatt cacggcgctc tcccaggtca
     1981 atctgcgcgt catgccgcgt accgtgcatt cggtgatcgg tccgaatggc gcgggcaaga
     2041 caacgctgtt tcacgttttg acgggtacgt tgcccatcac cgaagggaag atcatcttcg
     2101 acggccacga cgtgacgcat gaacccgacc ataaacgcgt gcgccgtggc gtcgcgcgtt
     2161 cgtttcaggt gacgagcctg ttcgccaatc tgagcgtgcg cgaaaatcta cggctcgccg
     2221 cgcaaggtgt cgacgcggtg cgtgcgttga acgcgtggac gccgccgcat ggtgcactca
     2281 cccacgcgga catcgtcgac agcgtccttg aacgcctcgc attgcagcgc tttagcgagg
     2341 tgcccgcggg cgcgttgtcg catggtcagc agcggcggct tgaagtcggc atggcgctcg
     2401 cggcgcggcc caaggcgatc tttctggatg agccgacttc cggcatgggc atcgacgacc
     2461 tcgacgacat gaagcaattg attcgcggcc tgcgcgatga ttacaccgtc gtgctgatcg
     2521 aacacaacat gggcatcgtg atggacatct ccgacacgat cacggtcatg cagcaaggac
     2581 gcgtgctggt ggaagggcgc ccggacgata ttcgcggcga cgagcgggca cgcagcgcct
     2641 atctcggcaa catgatcacc ggaggccgcg catgattctg gacgtgcagc agattcacgg
     2701 cttctacggc aagagccaca tcctgcaagg cgtgtccctg caaatcaacg acggcgagac
     2761 ggtcacgctg ttaggccgta acggcgcagg taagtcgacc acgctcaagg ctattgccgg
     2821 tgtcgtcaca ccacagcgcg gtacggtgac gttcaacggc gttgccatca gtggcatgcc
     2881 gccgcacaag atcgcctcgc gtggcgtgtg cttcgtgcct gaacatcgcg gtgtctttcg
     2941 tctgctgact gtcgaagaaa acctgcgcct gggcgcgcgc aaggattcgc cgtggcaact
     3001 cgacgacatc taccgcattt ttccgcgcct gaaagagcgg cgtgcgaatg gcggcgccca
     3061 actctccggc ggcgaacagc agatgctcgc gatcggccgt gcgttgatga atcatccgcg
     3121 gctgttgatg ctcgacgaac cggtcgaagg tctcgcgccg gtgatcgtcg aagagatcgt
     3181 cgcgcaactc aagttgattc gtgaagcagg cgtcgcgatt ctgctggtcg agcagaacct
     3241 ggaagtctgc acgcaactcg cggatcgcca ctacatcatc gaacagggcg tgatcgtcta
     3301 cgaaggcgac aacgctacgt tcgccgccga cgattcgatc aaggaccgat atttgggcgt
     3361 cggcgtcgtc tgacgcgcac tctgtcttca ctacgaggaa gcatcgatgc aagttcatga
     3421 atcaacaacc gtccggacgc tcgccgatct gcgtatcgac ggcacgcggc tctgggacag
     3481 tctcatgcaa ctcgcgcaaa tcggcgcgac agataaaggc ggtgtgtgcc ggctcgcact
     3541 gaccgaactg gaccgcaagg cgcgcgacct ctttatcgcg tgggcgaagg agattggctg
     3601 cagcgtgcgg atcgacgcga tcggcaatat cttcgcgcgt cgcgcgggtt tgcgtgacga
     3661 cctgccgccc gtgatgaccg gcagccacat cgatacgcag cccacgggcg gcaagttcga
     3721 tggtaactac ggcgtgctgg ccggtctgga agtattgcgc acgctggtcg atgccaacgt
     3781 gcggacgctc gcgccgctgg aagtcgcggt atggaccaat gaagagggtt cgcgtttcgt
     3841 gccggtgatg atgggctcgg gcgtgttcgc cggtgcattc acgttggagc atgcgctcga
     3901 acaacgcgat cgcgagggca tttcggtgcg cgacgcactc gcgtcgatcg gctatgcggg
     3961 cgaaaacggt acgcctcatg ccgttggcgc gtatttcgaa gcgcatatcg aacagggtcc
     4021 tgtgctggag gcgcatgaca cgacgatcgg cgtcgtacag ggtgcgttag gtcaacgctg
     4081 gtacgacgtg acggtgcacg gcatggaagc gcatgcgggt cccacgccga tggagttgcg
     4141 ccgcgacgcg ttgctcgtcg cggcagatct gattcacgcg gtgaatcgca ttgctctcga
     4201 ccacgcgccg catggacgcg ggacggtcgg ctggctcggc gtgcatccga attcacgcaa
     4261 cgtgattcct ggccgcgtta cccttacggt cgacctgcgc gcagccgacg atgcctcgct
     4321 caccgagatg gacgccgcac tacgcgctgc ttgcgcggcc gcgggtaagc aggcaggcat
     4381 caccgtcgac gtcgaacaag tggtgtattt tccgccccaa ccgttcgcgg cggatctggt
     4441 cgctgcgatt caggagggtg cggacacgct cggtttttcg tcgatggacg tcatcagcgg
     4501 cgctggtcac gacgccgtct atctcgcgcg cgtggcgccg gccgccatga tctttgtgcc
     4561 gtgcaaggac ggcatcagcc ataacgaaat cgaagacgcg cgcgcggatc acctcgaagc
     4621 cggctgcaac gtcctgttgc aggcaatgct gaacgccgcg caaacggcag ggagcacgca
     4681 ggtatgaaga ttctgattgc aaggatgaac cacgagacca acacgttctc gccggtggcc
     4741 acgccgcttg cagcgttcgg ccgcaacggt ccgagctatg gcgaagacgc gctcaacgaa
     4801 aacaagggca tgcagacggc aatggccgcg ttcattgacg cagccgagcg cgagcatgcc
     4861 gcgatcgtga cgcccatttc cgcatcggcg aatccgagcg gcccggtcga ggccgccgct
     4921 tatgacgcga tctgcgacgc gatcgttgcg gccgcagcag gctgcgatgc cgtgatgctc
     4981 gatctgcacg gcgcgatggt cgccgagaat tcgaacgacg gtgaaggcga tctgctcgaa
     5041 cgcctgcgcg ccgcattgcc caatgcaccg attgcggtcg cactcgatct gcatggcaac
     5101 gtcacgcaga agatggtcga taacgccgac gtgatcgtca gtttcaaaac ctatccgcac
     5161 gtcgatatgt acgagacggg cgcgcatgcg gcgcgtctga tgttcgacct ggtccatggc
     5221 aaggcgaagc cggtgatcgc atggcgccaa ccgccgttgc tcacgcatac gctgcgcagc
     5281 gcgacg
//
LOCUS       DATASH010001009         1772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5909565,
            whole genome shotgun sequence.
ACCESSION   DATASH010001009 DATASH010000000
VERSION     DATASH010001009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5909565"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VHV99_26855"
     CDS             <1..560
                     /locus_tag="VHV99_26855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen debranching enzyme GlgX"
                     /protein_id="HEX3639038.1"
                     /translation="WERASSPDGRQMTDFVARVIALRKHHPLLRETRFLFGDREVLPG
                     LFDVGWFDEHGDPLTIEAWEDPEGRAFTLRRAGPGLNGETEVLLMMLNAHEEARRFTP
                     PAPHLEWHVLLDTAEPERGPHRLAAAEIEVAAHGLVMLAAQPVGEADWQAGWKAGAQH
                     GLRLLTALPPDPGGHEASSDTPPSA"
     gene            763..>1772
                     /locus_tag="VHV99_26860"
     CDS             763..>1772
                     /locus_tag="VHV99_26860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX3639039.1"
                     /translation="MSETPIDPHAHHHAHCLPFSAQLLGAAGTQPRTRFRFWAPSCKT
                     VQVAIESGPAQGMHDMTPTGNGWFEATVEGGAGMLYRFRLDDGQLVPDPASRFQPQDV
                     HGPSEVIDPRAYRWEHTTWHGRPWEETVLYELHVGAMGGYAGVRKRLPELAALGVTAI
                     ELMPLNDFPGNHNWGYDGVLPYAPDSAYGRPEELKALVDAAHGLGLMVFLDVVYNHFG
                     PDGNYLHEYARSFFREGTHTPWGPAIDFARSEVSDFFTDNAVYWINEYRLDGLRFDAV
                     HAIDNHAWLRDLSVHIRAKVQHGRHVHLVLENEHNSASLLETHFDAQWNDDAHNTLHV
                     LLT"
BASE COUNT          333 a          556 c          596 g          287 t
ORIGIN      
        1 actgggagcg tgcaagctcg ccggacggcc ggcagatgac cgacttcgtc gcgcgcgtga
       61 tcgctttgcg caagcatcat ccgctgctgc gggaaacgcg ttttctgttc ggcgaccgtg
      121 aagtgctgcc gggactgttc gatgtcggct ggttcgacga acacggcgac ccgctcacca
      181 tcgaggcgtg ggaagaccct gaaggccgcg cgttcacgct gcgtcgcgcg gggccgggct
      241 tgaacggcga aacggaagta ttgttgatga tgctcaacgc acacgaggaa gcacggcgct
      301 tcacaccgcc cgccccgcac ctggaatggc acgtgctgct agatactgcc gaaccggagc
      361 gcgggccgca tcggctggcg gcggccgaga tcgaggtggc cgcgcacggg ctcgtgatgc
      421 tggccgcaca gccggtgggc gaagcggact ggcaggcggg ctggaaagcc ggcgctcagc
      481 atgggctgcg ccttttgacg gcgctgccgc cggatccggg cgggcatgag gcgagcagcg
      541 acacgccgcc gagcgcgtag acacgagcgc cgtaaccgcg aagttgcggg gaggcaacgg
      601 cgcggcggcg gccggatcgc agcgggatcg cagtacgaac acacatgcat ggcggccgcg
      661 tgttcaggtg cgcttgctcg acaagcgctt cgtagcgaag cgcacaacgc aaggcgcaca
      721 agcacaacgc ggctcgacag taccctggaa tggtgaagaa tcatgtctga aactccgatc
      781 gaccctcacg cgcatcatca cgcgcactgc ctgccgttca gcgcgcaact gctcggcgcc
      841 gcaggcacgc aaccgcgcac gcgattccgt ttctgggcgc cttcgtgcaa aacagtgcaa
      901 gtcgcgatcg aaagcggacc ggcgcaaggc atgcacgaca tgacgccgac cggcaacggc
      961 tggttcgaag caaccgtgga gggcggcgcg ggcatgctgt accgcttccg gctcgacgac
     1021 ggacagctgg tgcccgatcc ggcgtcgcgc tttcagccgc aggacgtgca tgggccgagc
     1081 gaagtcatcg acccgcgcgc gtaccgttgg gagcacacga cctggcatgg ccggccttgg
     1141 gaagaaacgg tgttgtacga gttgcacgtc ggcgcaatgg gcggttatgc gggcgtgcgc
     1201 aagcgcctgc cggaactcgc cgcgctcggc gtcacggcca tcgagttgat gccgctgaac
     1261 gactttcccg gcaaccacaa ttggggctat gacggcgtgt tgccgtatgc gcccgactcc
     1321 gcttacggcc ggccggaaga actcaaggcg ctggtcgatg ccgcgcacgg tctcggcctc
     1381 atggtgtttc tcgatgtggt ctataaccac ttcggcccgg acggcaatta tctgcacgaa
     1441 tatgcccgct cgtttttccg cgaaggcacg cacacgccat ggggcccggc aatcgatttc
     1501 gcgcgcagtg aagtgagcga cttcttcacg gataacgccg tctactggat caacgaatac
     1561 cggctcgacg ggctgcgttt cgatgcggtg catgccatcg acaaccatgc gtggctgcgc
     1621 gatttgtccg tccatattcg cgccaaagtg cagcatggcc ggcatgtgca tctggtgctg
     1681 gaaaacgagc acaacagcgc gagcctgctg gaaacgcatt tcgacgcgca gtggaacgac
     1741 gacgcgcaca acaccttgca cgtgctgctg ac
//
LOCUS       DATASH010001010         2898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_965449,
            whole genome shotgun sequence.
ACCESSION   DATASH010001010 DATASH010000000
VERSION     DATASH010001010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965449"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VHV99_26865"
     CDS             complement(<1..559)
                     /locus_tag="VHV99_26865"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017773574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX3639040.1"
                     /translation="MSEKKSGLRQGLTNYGDEGFSLFLRKAFIKGAGYTDDALSRPIV
                     AIANTGSAYNPCHGNAPQLIEAVKRGVMLAGGLPVEFPTISIAESFSHPTSMYLRNLM
                     SMDTEEMIRAQPMDAVVLIGGCDKTVPAQLMGAAAAGVPAIQLVTGAMLTGSHRGERV
                     GACTDCRRFWASFRGDQIDEAEIEAV"
     gene            703..1701
                     /locus_tag="VHV99_26870"
     CDS             703..1701
                     /locus_tag="VHV99_26870"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEX3639041.1"
                     /translation="MKHVVAYRSLPPAVLGLLREHCSVELVDVSSGDLAPFRRALGTA
                     HGMVGNNLKITPEILDAAPRLEVASTISAGFDAFDVPELSRRGIVLTNTPEEVAETTA
                     DLVFSLILATARRISELADWTRRGQWTRTIGEAQFGVDVHHKTLGIVGLGRIGSAVAR
                     RAALGFGMNVIYSNRSRNLEAEERYGAQWRPLPELLATADFVCVLVPLSPATEGLIGS
                     AELGAMKPSAILINCARGQVVDEAALIEHLRENRIRGAGLDVFVREPVSPDSPLLQLP
                     NVVAVPHIGSATQQTRENMAMRAVRNLIDAFDGQLSSTCVNPEALHARSPAQPIAG"
     gene            1743..2672
                     /locus_tag="VHV99_26875"
     CDS             1743..2672
                     /locus_tag="VHV99_26875"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX3639042.1"
                     /translation="MTSVNRPRGVYSPVVTPFTKDLLPDHDRYVRHCRWLIEQDVGLA
                     VFGTNSEANSLAVSEKMKLLETLVGAGIPLDRLMPGTGCAALTDTVELTRHALSLGVN
                     NVLMLPPFYYKGVTDEGLFRSYAQIIERVGSDALRIYLYHIPPVSQVPLSLALIERLL
                     AAFPGVIAGVKDSSGDWSNTQAMLERFQPQGFDVFAGSETFLLATMRAGGVGCITATG
                     NVNPAAIVQLYRHWQAADADARQAALDATRAAFAKFPMIPALKAAIGVYSNDPEWGVV
                     RPPLVDLTPVQCDALAAALSEIGFSMPGIEVTA"
     gene            complement(2744..>2898)
                     /locus_tag="VHV99_26880"
     CDS             complement(2744..>2898)
                     /locus_tag="VHV99_26880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX3639043.1"
                     /translation="FPDDQVLNFRRLVDTQLSFADGQLTVPTRPGLGFDFDEAALNEY
                     ALDDWA"
BASE COUNT          513 a          985 c          844 g          556 t
ORIGIN      
        1 tgaccgcttc gatttcggct tcgtcgatct ggtcgccccg gaagctcgcc cagaagcgac
       61 ggcagtccgt acatgccccc acccgttcgc cccgatgcga gcccgtgagc atggcgcccg
      121 tcaccagttg aatcgccgga acgcccgccg ccgccgcacc catcaactgg gccgggacgg
      181 ttttgtcgca gccgccgatc agcaccaccg catccatcgg ctgggcccgg atcatctcct
      241 ccgtgtccat cgacatcaga ttgcgcaggt acatgctggt cggatgcgaa aagctctcgg
      301 cgatcgaaat cgtcgggaat tcgacaggca gcccgcccgc cagcatcacg ccgcgtttta
      361 ccgcctcgat caactgcggt gcgttgccgt ggcacgggtt gtaggcactg cccgtgttcg
      421 cgatggccac gataggacgc gacagcgcgt catcggtata gcccgcgccc ttgatgaacg
      481 ccttgcgcag aaacagcgag aaaccctcat cgccgtagtt cgtcaggccc tgccgcaaac
      541 cgcttttctt ctcgctcatc accgctccag aattttcgat aggattatca ataatcttga
      601 cggcgatctt accgatactt atagtctctc cgcaagcgat tctttttgtt gcggtcgatg
      661 ccgcctggca actctctcta acagcattcc atcgaggtct taatgaagca tgtcgtggcc
      721 taccgttcgt tgcctcctgc cgtgctgggg ctacttcgcg agcactgttc cgtcgagctg
      781 gtcgacgttt cctccggcga cctcgcgccg ttcaggcgcg ccttgggcac ggctcatgga
      841 atggtcggca acaatctcaa gataacgccc gagatccttg atgcagcgcc ccggctcgag
      901 gtcgcgtcca ccatttcggc aggcttcgat gcgttcgacg tgcccgagct ctcgcgccgc
      961 ggcatcgtgc tgacgaatac gccggaagaa gtcgcggaaa ccaccgccga cctcgtgttc
     1021 agcctgatcc tcgcgaccgc ccggcgcatt tcggaactcg ccgactggac gcggcgcggc
     1081 cagtggacgc gtacgatcgg cgaggcgcaa ttcggtgtcg acgtgcatca caaaacgctg
     1141 ggcatcgtcg ggcttgggcg cattggcagc gcggtcgcca ggcgcgcggc gctcggcttc
     1201 ggcatgaacg tgatctattc caaccgctcc cgcaacctcg aagcagagga gcgctacggc
     1261 gcgcaatggc ggccgcttcc cgaactgctc gcaactgcgg acttcgtttg cgtgctggtg
     1321 ccgctctcgc ccgccaccga gggcctgatc ggcagcgccg agctgggggc catgaagccg
     1381 tcggccatcc tcatcaactg cgctcgcgga caggtcgtcg acgaagccgc cctcatcgag
     1441 catctgcgcg aaaaccgcat ccgcggcgcc ggtctcgatg tgttcgtacg cgaaccggtg
     1501 tcgccggact cgccgctgct ccagttaccg aacgtggtcg cggtgccgca tatcggctcc
     1561 gctacgcaac agacgcgcga gaacatggcc atgcgcgcgg tacgcaatct catcgatgca
     1621 ttcgacggcc agctctcgtc gacctgtgtc aatcccgaag cgctccacgc ccgctcgccg
     1681 gcgcaaccga tcgccggctg aatggcgcga atcaccctca ccccaatcaa aaggaacacg
     1741 cgatgacttc agtcaaccgg ccgcgcggcg tctactcgcc cgtggtcacg ccgttcacaa
     1801 aagatcttct tcccgatcac gaccgctatg tccgtcattg ccgatggctg atcgaacagg
     1861 acgtcggact cgctgttttc ggcaccaact cggaagcgaa ttcgctcgcc gtcagcgaga
     1921 aaatgaagct gctcgaaacg ttggttggcg caggcattcc gctcgatcgt ctgatgccgg
     1981 gcaccggctg cgctgccctc accgacacag tcgaactgac ccgccatgcg ctttcgctgg
     2041 gcgtgaacaa cgtgctgatg ctgccgccgt tctactacaa gggcgtcacc gacgaaggcc
     2101 tgttccgcag ctatgcgcag atcatcgagc gcgtgggttc cgacgcactg cgcatttatc
     2161 tgtatcacat cccgcccgtg tcccaggtgc cgctcagcct cgcgctcatc gagcggctgc
     2221 tcgctgcttt cccgggcgtg atcgccggcg tgaaggacag ttcgggcgac tggagcaaca
     2281 cgcaggccat gctcgagcgc ttccagccgc agggcttcga tgtgttcgcc ggcagcgaaa
     2341 ccttcctgct ggctaccatg cgggcgggcg gcgtcggctg catcacggca accggaaacg
     2401 tcaatccggc ggctatcgtg cagttgtatc gtcactggca agcggccgac gccgatgccc
     2461 gtcaggcagc actggatgcc acgcgcgcgg cgttcgccaa gttcccgatg atccccgcgc
     2521 tcaaggcagc gatcggcgtg tattcgaacg atcccgagtg gggcgtcgta cgtccgcctc
     2581 tcgtcgatct gacgcccgtg caatgcgatg cgctcgccgc cgcactcagc gaaatcggct
     2641 tctcgatgcc gggtatcgaa gtcaccgcct gatgccctga agcaacaccc actggttgca
     2701 tgcgcaacca gtgggtgttt cccggcgttt tactgcttcg gcttcacgcc caatcgtcga
     2761 gcgcgtattc gttcagcgcg gcttcatcga agtcgaatcc gaggcctgga cgcgtcggca
     2821 cggtcagctg accgtctgcg aacgacagtt gcgtatccac cagccgacgg aaattcagca
     2881 cctgatcgtc aggaaaaa
//
LOCUS       DATASH010001011         3562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1164686,
            whole genome shotgun sequence.
ACCESSION   DATASH010001011 DATASH010000000
VERSION     DATASH010001011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1164686"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VHV99_26885"
     CDS             <1..547
                     /locus_tag="VHV99_26885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX3639044.1"
                     /translation="RDAGKLLRLTGTLPRIAVNVSPQQFQRHNLFETIREALEEAALD
                     PSYLELEITEGALLGDTELALQTLHALRELGVEVAVDDFGTGYSSLAYLTRFPLNRLK
                     IDRSFVMRMSTDPQCAALVGAIIAMAHALKLRVTAEGVETAEQAAQLEALGCDEAQGF
                     WFSRPVTIAALRNLLRPLGTA"
     gene            complement(779..1288)
                     /locus_tag="VHV99_26890"
     CDS             complement(779..1288)
                     /locus_tag="VHV99_26890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEX3639045.1"
                     /translation="MQSPAIEHSAAFGQRTFNIFTDLQLDGLACLQKLGELNLAALKA
                     TVTESKNALSAGQFNVAPFAGAAEIQQQLVQRTLSYAQHVKEIDAQFTAALNKAGEAL
                     REEFGASAAQFGSGFGQTTPFSSDSAFGAMQSVMASMIQSGNAMQDSIKRVSGAMTGT
                     AITTADNAA"
     gene            complement(1470..2603)
                     /locus_tag="VHV99_26895"
     CDS             complement(1470..2603)
                     /locus_tag="VHV99_26895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEX3639046.1"
                     /translation="MKMNEDFLKSMKDAFDLLQTKGAQEATAAVQRALAGASAEAGNA
                     HPSHTPQGVNEWARAWTQAQTQPQNQAQSRAHTTPHAPAERTFDADTPGTFSTHTFSN
                     SAGQRQYKLYVPAVYNSEPLPLIVMLHGCTQNADDFAAGTRMNEMAERHGFIVVYPNQ
                     SQAANHSKCWNWFKPADQQRDQGEPSLIAGITREVMARYRVDPARVYVAGLSAGGAMA
                     DVMLKTSPDLYAAACVHSGLAYGCAKDLPSALAAMKGGKAHRNRSSVEPQRPLIVFHG
                     DADTTVHPSNATALVAGFDAGATTIGTPSHAAGTTSGQRGCTVQRLVAANGVEAEHWS
                     IHGAGHAWAGGSQRGSYTDASGPDATAEMLRFFLAHPRASATH"
     gene            complement(2718..3143)
                     /locus_tag="VHV99_26900"
     CDS             complement(2718..3143)
                     /locus_tag="VHV99_26900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEX3639047.1"
                     /translation="MANLKLISTSRPKGGETEKITINLGPIDLGQIDLLVEEGFYSNR
                     TDLIRTAIRNQLAVHAQVVTETVTRRAMVLGLQHFSRQDLEAAQAANERLDIHVLGLA
                     SIAADVPPELAAATIASVVVLGAFHASPAVKAALAGRIR"
BASE COUNT          615 a         1056 c         1160 g          731 t
ORIGIN      
        1 ccgtgatgcc ggcaaactgc tgcggctgac cggtaccttg ccgcggatcg cggtgaacgt
       61 ctccccgcag caattccaac ggcacaatct gttcgagacg atccgcgagg cgctcgaaga
      121 ggcggcgctc gacccttcct acctcgagct ggaaatcacc gagggtgcgc tgctcggcga
      181 caccgagctg gcattgcaga cgttgcatgc gttgcgcgaa ttgggcgttg aagtcgcggt
      241 ggatgacttc ggcactggct attcgagtct cgcctatctc acgcgctttc cgctgaatcg
      301 cctcaaaatc gaccgctcgt ttgtgatgcg catgagtacc gatccgcaat gcgcggcgct
      361 ggtcggcgcc attatcgcaa tggcacacgc gttgaaactg cgcgtgacgg ccgaaggcgt
      421 ggagaccgcc gaacaggccg cgcagctgga agcacttggc tgcgacgagg cgcaaggatt
      481 ctggttctcg cggcccgtca cgatcgcagc gttgcgcaat ttgctgaggc cgttgggcac
      541 tgcttgaccg tccggctaat accgcagccg tggaatcctc cggtaactta gcccgtcaat
      601 ccttcagtcg atgcagtgca tcgctgctgc agcggcggcg gtacgcaaca tatcgaggtt
      661 gagagtcgga ctcgcgcagc gcaaggcgtg tgtagcgcgg ctaaagcgga aaagagaaaa
      721 ggggccaatc ggcccctttc ctttagtcgc cataccgcgc gttacgacgc gagactgact
      781 atgcagcgtt gtccgcggtg gtaatcgccg tgcccgtcat ggcaccggat acacgcttga
      841 tgctgtcctg catcgcgttg cccgactgaa tcatcgaagc catcacggat tgcattgcgc
      901 caaatgcgct gtccgaactg aacggcgtgg tctggccgaa gcccgaaccg aactgcgccg
      961 cgctggcgcc gaactcctcg cgcagggctt caccagcctt gttcaacgcc gcggtgaatt
     1021 gcgcgtcgat ttccttgacg tgctgtgcgt acgacagggt gcgttgaacc agttgctgct
     1081 gtatctcggc cgcgcccgca aacggcgcga cgttgaactg gccggccgac agcgcgttct
     1141 tgctttcggt gacggttgcc ttcagggccg cgagattgag ctcgccgagt ttttgcaggc
     1201 aggccaatcc gtcgagctgc aagtcggtga agatgttgaa agtgcgttgg ccgaaggcgg
     1261 cgctatgttc gatcgcgggg ctttgcataa gtgtgatctc cgaagtgacc cagcgcaggc
     1321 gtccaaagga tttcggaggc caggtctcaa gcgagacggc aggtcgacac agcgcagtgt
     1381 tttttgctgc gctgcaacat gagccactct atacctttgc ggctggatgt acagcacttt
     1441 cgtcgcgatc tttcgtcagt tgaaagtaat tagtgcgtgg ctgacgctcg cggatgggcg
     1501 aggaagaagc gcagcatttc ggccgtcgcg tcggggccag atgcgtccgt gtaagagccg
     1561 cgctgactgc cgcctgccca tgcgtggccc gcgccatgaa tcgaccaatg ctccgcttcc
     1621 acgccgttcg ccgcgacgag ccgttgcacc gtgcagccgc gctggccgct cgttgtgcct
     1681 gccgcgtgcg acggcgtccc gattgtcgtg gcgccggcgt cgaagcctgc tacgagcgct
     1741 gtggcgttcg agggatgcac ggtcgtgtcc gcgtcgccat gaaacacaat gagcgggcgt
     1801 tgcggttcga cggatgagcg gttgcgatgt gccttgccgc ccttcatcgc ggcgagcgcc
     1861 gagggcagat ccttcgcgca tccgtacgcg aggccggaat gcacgcaggc tgccgcatac
     1921 aggtcgggcg aagtcttcag catgacgtcc gccatggcgc cgcccgccga tagacccgcc
     1981 acgtacaccc gtgccggatc gacgcgatag cgcgccatca cctcccgcgt gataccggcg
     2041 atcaacgacg gctcgccctg atcccgttgc tggtcggcag gcttgaacca gttccagcac
     2101 ttcgaatggt ttgccgcctg cgactgattc ggatacacga cgatgaaacc atggcgctcg
     2161 gccatttcgt tcatgcgcgt gccggccgcg aagtcgtcgg cgttttgcgt gcagccgtgc
     2221 agcatcacga tcagcggcaa cggttcgctg ttgtagacgg cgggtacgta gagcttgtat
     2281 tggcgttggc ccgcgctgtt gctgaaggta tgggtgctga aggtgccggg cgtgtcggcg
     2341 tcgaaagtgc gctcggcggg cgcgtgtggc gtggtgtggg ctcgggattg cgcctgattc
     2401 tggggttgag tttgcgcttg cgtccacgct cgtgcccatt cgttgacacc ctgcggcgtg
     2461 tgcgatgggt gtgcgttgcc ggcttcggcc gaggcgcccg cgagcgcacg ctgaaccgca
     2521 gcggtcgctt cctgcgcccc tttcgtttgc agaaggtcga aggcgtcttt catcgacttc
     2581 aggaaatctt cattcatttt catgatcgta agcggttata ggttgagtgg cagaaggtgc
     2641 ggcggggccg atgagcgtca cgggttcaac gtggtcagcc acgtcttcaa ccataagatc
     2701 agccacgcga ggcgcgccta cctgatgcgg ccggcgagtg ccgccttgac cgcgggcgac
     2761 gcgtgaaatg cgccgagcac caccaccgat gcgatcgtgg ccgccgcgag ttccggcggc
     2821 acatcggctg cgatgctggc gaggccgagc acatgaatgt ccagtcgttc gttcgcggct
     2881 tgagcggctt cgaggtcctg cctcgagaag tgctgcaagc cgagcaccat cgcgcgccgg
     2941 gtcaccgttt ccgtgacgac ctgcgcatgt acggcgagct ggttgcggat cgccgtgcga
     3001 atcaggtcgg tgcggttcga gtaaaaacct tcttcgacga gtagatcgat ctggcccaag
     3061 tcgattggac caaggttgat ggtgattttt tcggtctcac cgcctttggg gcgcgaggtg
     3121 ctgatgagtt tgaggttggc catttctgtc ccgttcggca tccggagaac atccggatgc
     3181 catccaatta acatccaatg ggatggttat acgcttgggg ttgggaagac gtcaacgggg
     3241 aaattgaggg ccggtagagg ttttttttgg ggccgtagct gtgctgacgg caacgtctgc
     3301 agctttcccc tgcgccggtt gcgcgtcggg gcccggagaa cgcgcggcga acccgcgttc
     3361 cagtcagatg gtccgcgcgg gatgccttta caatcctgcg ctcagatccg caaacgggaa
     3421 tcgcgccatg aagcacctca tcgcatcgtc actcgtcgtc gcccttgccg ctgtgagcgc
     3481 gcacgcgata gctcaaacat caagcagcaa tgcgacgcca aaacaatcgt gcgcgatcgc
     3541 gtacgtcacg ggtattggcg ga
//
LOCUS       DATASH010001012         6595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4921096,
            whole genome shotgun sequence.
ACCESSION   DATASH010001012 DATASH010000000
VERSION     DATASH010001012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6595
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4921096"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3492)
                     /locus_tag="VHV99_26905"
     CDS             complement(<1..3492)
                     /locus_tag="VHV99_26905"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX3639048.1"
                     /translation="MKLPVPNKQDTTVILETYGKNEIPFRFADVKIPINGPDVVVKTA
                     DGHTYTFPFAAQLATMGKHVFTLVFADGKEIPSDQLLSYATGVSAAPNAAAPQTHHEG
                     DSNGTAGHDAPKQVVKEVVKEVTKEVVKEDLIVADSSSQDGGAGAEQTDAAHKPLPVV
                     NAADFAKHRFDDAPLPQVSHIAGMPPKPDPQPHDDPTPPQPEPAPEPDPTPQPDPTPT
                     PDPTPTPDPTPQPDSTPQPDPTPPSLKDVLLLQTVGTADQNTHEYYVGAGSDAARTDG
                     RISTQYSARDVDLSHETTGWTIHADTTVGAGSAGSMVRIVNAGSARAILDVSIDPALA
                     AKGVSVVQWDSPDGMALGLKVNEFALRYPSAQGAVDGIFKVHLDYVDAASGESAATDI
                     AFAITDAPTALHDQNDTYQLSRQDNDVVIHGGAGDDTIIAGHSSGVYDGGGGVNTLDY
                     SDFSSTLVVDLSADVRAALGISPVGDGSGIDMGRVKTGDTTHAIENIQIIKGGDGDNT
                     FIGNQFDHEFVGGAGTNTFVANGGNNIYTGGASGLNTIDYSRAGGTTFETATLGASGR
                     QFVLNGVDVDMANGVTANNGWLDANGNAGADHFSGVNQIIGTRYNDRIKMGDGDVTLV
                     GGAGDDLIIAGAGNDKIDGGLGFNVVSYAGLTGQVDVDLNGTTGLASKSNGAVDTLTH
                     IQAVIGSAGGGTLTGLVNGSNLLIGTDGDTNFATTAGTNQLYGGAGKNTFDVIQGTNA
                     IYGGATLNTVNAQGGTNTFFAGDGVNVFNADGGKNVYHGGAGSDTMIAINGGNNTMIG
                     GSGHSVFSATGGGTNTIDGGAGDMTVYGGGANNTVKGGGGGRTTVDYSGYQGSGHLDI
                     TLDRNLALIGNDFVDRLYDVDVIVGASGGNSVIRGARDRGIEIYASGDNNWLSGGNGN
                     NVLSVSGNNNWLVGGRGNNVFDGGSGVNNTVDYSGATGAVNVNLNTGVVAQNGYGFTD
                     RIANIQRIVGSNASGNVFEGRDGTNNWIDLGNGTNNKVIGTVGSDTYVSHSTTNTLDY
                     SHLKAGINLDMSKGIVRKGMNGTDTLVGSFGNMIGTDYGDTIVVSGNFPIQVKAGAGD
                     DKIVMKGLGYPTTIDGGGGFNELDATDAAGGSYMAFTINADGRSGNVRGFNSADWTTG
                     NVTSWYTSPATFKNIDVI"
     gene            complement(3563..4861)
                     /locus_tag="VHV99_26910"
     CDS             complement(3563..4861)
                     /locus_tag="VHV99_26910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HEX3639049.1"
                     /translation="MKEKFSLIVIRIRQGIALCRPYAPCGMTGVALLGFTLLAVWSTM
                     FKLDVLVRGPGVVRVESHNIVVQHADGGQIEKLLVREGQTVRKGQLLAVMDNTYVSEE
                     FARNSAALSTLRLREQRLEAELAHTRFDPAMPADAEQAKAVAGEKALYESRQLALDQS
                     ASISQVQADQRLAQSAELRTRIGDLDKEVALMQQQVDMIAPLVRKGAAAEGVLLQKRS
                     DLQSAQSALNEARAKVPRLVAEEREYRLKVEQIKTDFMSDAQKQLSQARDELAQVNAK
                     ANASQDRRKQAQIESPADGVIQRVVNPHAGAVVRPGGDLFEIAPTDVPLVAQVRIRPE
                     DRDKIWQGMPARVHVNAFGNSYADTLDGRVAVVSADALGDDRTGRFYEVTVEVPKSNL
                     KKPIYPGMAVESYLLAGHRSLAQYLLKPLMSGASAALSES"
     gene            complement(4915..5424)
                     /locus_tag="VHV99_26915"
     CDS             complement(4915..5424)
                     /locus_tag="VHV99_26915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639050.1"
                     /translation="MASGSKNHIWPAYVDMMTVLLMVYLLLSMIFQVLAALAGPPVES
                     APATSSSESSSPAGAVELTDKLTRQPDDFVASVGRDENFLQPTARAAVRGWMSSHAQA
                     IRQRGLLVFSLTGATDAPLGVRMRLQYERSLMVVAIADELGIPRDKIDLRNPVPDDGT
                     QDSIVLRLR"
     gene            complement(5424..6116)
                     /locus_tag="VHV99_26920"
     CDS             complement(5424..6116)
                     /locus_tag="VHV99_26920"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX3639051.1"
                     /translation="MQLTKAKQQFKSITGLDAVPQQRDRVKELPPVNLQPLIESAADA
                     NLAVKARRANVESNIALVNVERASRYPRVDIVATVRKPFQNVPSPGSSVMLQVTFDLF
                     DGGLQKSRVNEAVSAKYVAQQQLEQELRNVNDQTEALRSDWDSARDQWTLSVDGEKQA
                     LRTLELYADEFRLGTRQLSDLINAQVDLFSARSDREQAESGYYLATLALYNMDAKTMD
                     GLRALNLIKEEA"
     gene            complement(6138..>6595)
                     /locus_tag="VHV99_26925"
     CDS             complement(6138..>6595)
                     /locus_tag="VHV99_26925"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX3639052.1"
                     /translation="TKIARGQADEAQARVSGARAAYYPKVGVIAKTPDRIYGATQGYG
                     RTFDQYSQVNGTIQYTVFDFGRRKNQVSSAALSAEAAESAYQGEVEMLAYSTSRAYLD
                     VVRYQHLKKIAEDYVAQVQRLNSVIEERVAGGLAPQSDAIRGQLALTSA"
BASE COUNT         1243 a         2051 c         1854 g         1447 t
ORIGIN      
        1 aataacgtcg atattcttga acgtagcagg acttgtgtac caagacgtaa cattgcctgt
       61 ggtccagtcc gcggaattaa agccccggac gttaccgctt cgaccatccg cgttgattgt
      121 gaacgccatg taggaaccgc ctgcggcgtc ggttgcgtca agttcgttga aacctccgcc
      181 cccgtcgata gtggtcggat atccgagacc cttcatcaca attttgtcgt caccagcacc
      241 agctttcacc tgaatgggaa agttaccgct caccacgata gtgtcaccat aatccgtgcc
      301 tatcatgttg ccgaaagagc cgacgagggt atccgttcca ttcatacctt tccggacaat
      361 gcccttagac atgtcaagat taattcctgc tttgaggtgc gaatagtcga gggtattggt
      421 tgtgctatgg cttacatagg tatcgctacc caccgtcccg atcaccttgt tattggttcc
      481 gtttccgagg tcgatccagt tattcgttcc gtcacgccct tcaaaaacgt tacccgacgc
      541 gttactgccg acgattctct gaatattagc aatcctgtcc gtgaagccgt agccattctg
      601 tgccaccacg ccggtattca gattgacgtt cacggcgccg gtcgcgccgc tgtagtcgac
      661 tgtgttgttg acgcctgagc cgccgtcgaa cacattgttg ccgcgtccgc cgaccagcca
      721 gttgttgttg ccggaaacgc tcaacacatt gttgccattt ccgccagaca gccagttatt
      781 gtcaccggaa gcgtaaattt caatgccgcg gtcgcgggcg ccacgaatca cactgttgcc
      841 accgcttgcg cctacgatca catcgacatc gtacaaacgg tcgacgaagt cattgccgat
      901 cagcgccaga ttgcgatcga gcgtgatgtc gagatgcccg ctaccctggt atcccgagta
      961 gtccactgtc gtccttccgc cgccaccgcc cttcaccgtg ttatttgcgc caccgccata
     1021 gacggtcata tcgccggcac caccgtcaat cgtattggta ccaccgccag tagcactgaa
     1081 tacgctgtgg ccgctgccgc cgatcatggt gttgttgcca ccgttgatcg cgatcatcgt
     1141 gtcgctgccc gcgccaccgt gatacacgtt cttgccgccg tcagcgttga atacgttcac
     1201 accgtcgccc gcgaagaacg tgttggtacc gccttgcgca ttgacggtgt tcagggtcgc
     1261 gccgccatag atcgcgttgg ttccctggat gacgtcgaaa gtattcttac ccgcaccgcc
     1321 gtaaagttgg ttcgtcccag cggtcgtagc aaaattcgta tcgccatcgg tgccgatcaa
     1381 cagattgctg ccattcacta ggccggtaag cgtgccgccg ccggccgagc cgatcacggc
     1441 ctgaatatgc gtaagcgtgt ccaccgcgcc gttgctcttc gacgcaagcc cagtcgtacc
     1501 attgaggtcg acatcgactt ggccggtaag cccagcgtag ctcacaacgt tgaaacccag
     1561 gccgccgtcg atcttgtcat taccggcacc tgcaatgatc agatcatcgc ccgcgcctcc
     1621 aacgagggtg acatcgccgt cacccatctt tatccggtcg ttgtaacgtg tacctatgat
     1681 ctgattcacg ccggagaagt ggtccgcacc ggcgttgccg ttcgcgtcca gccagccatt
     1741 atttgcagta acgccattgg ccatgtcgac atcgacgccg ttcagcacga actgtcggcc
     1801 actggcgccg agcgtcgccg tttcgaacgt cgttccgccg gcacgcgaat aatcgatcgt
     1861 attcaggccg cttgcgccac ccgtatatat gttgttaccg ccattcgcca caaacgtgtt
     1921 ggtgccggcg ccgccgacaa actcgtgatc gaactggttg ccgataaacg tgttgtcgcc
     1981 gtcaccgcct ttgatgatct ggatgttctc gatcgcatgc gtggtgtcac cggttttcac
     2041 gcggcccatg tcgatcccac tgccatcgcc gaccggacta atgcccagcg ccgcacgcac
     2101 atccgccgac aaatcgacca cgagcgtcga cgaaaaatcc gagtagtcga gcgtgttgac
     2161 accgccgccg ccgtcataga ccccgctcga gtggcctgca atgatggtgt catcgccagc
     2221 cccaccgtgg atcacaacgt cgttatcctg acggctcaat tgataggtat cgttctgatc
     2281 atgcagcgcg gtcggggcat ccgttatcgc aaatgcgata tccgtggccg cgctttcgcc
     2341 cgaagcggca tccacgtagt cgaggtgaac cttaaagatg ccatcgaccg cgccttgcgc
     2401 gcttggatag cgcaatgcaa attcgtttac tttcagccca agcgccatcc cgtcgggcga
     2461 atcccactgc acgacgctca cgcccttggc tgcaagcgcc ggatcgatcg acacgtccag
     2521 tatcgccctt gcggaaccgg cgttcacgat acgcaccatg gaacctgcgg atccagcgcc
     2581 gaccgtagtg tcggcgtgaa tggtccaccc agttgtctcg tggctcagat cgacatcgcg
     2641 cgccgaatat tgggtcgaaa tacgcccgtc ggtacgggca gcatcgctgc ccgcgcccac
     2701 atagtattcg tgcgtgttct gatcagccgt accgactgtc tgcaacaaga gcacgtcctt
     2761 cagcgaaggc ggcgtcggat cgggctgcgg tgtcgaatcc ggctgtggtg ttgggtcggg
     2821 cgtcggtgtt gggtcgggcg tcggtgttgg atccggttgt ggcgtcggat ccggttcagg
     2881 cgccggctcg ggctgcggcg gagtgggatc gtcgtgcggt tgcggatccg gcttcggcgg
     2941 catgcccgca atgtgggaga cctgcggcag cggtgcgtcg tcgaaacggt gcttggcgaa
     3001 atccgccgca ttgacaacgg gcagcggctt gtgagccgca tcggtctgtt ccgcgcccgc
     3061 tccgccgtct tgcgacgacg agtcggcaac aataaggtct tccttcacga cttctttcgt
     3121 gacttccttg accacctctt tcaccacctg tttaggcgcg tcgtgtccgg cggttccgtt
     3181 gctatcgcct tcgtgatggg tctgcggcgc agcggcgttc ggcgcggcac tgaccccagt
     3241 ggcataactg agcagttggt ctgacggaat ctccttgccg tccgcgaata ccaaggtaaa
     3301 cacgtgcttg cccatcgtcg caagctgggc ggcgaatgga aatgtgtagg tatggccgtc
     3361 cgcggtctta acgaccacat ccgggccatt gatcgggatc ttcacgtccg cgaaacggaa
     3421 tgggatctcg ttcttcccat aggtctcgag aatgacggtg gtgtcttgtt tgttcggtac
     3481 tggcaatttc atatcatggc tccgtgaaga cgtcgcaggt agataagcgg tccgtcggca
     3541 tggttatcag ttagatcgaa aatcaggatt cagagagcgc agcggacgcg cccgacatca
     3601 gcggtttcaa cagatactgc gcaagcgagc gatgtcccgc gagcagatag ctttcgacag
     3661 ccatccccgg ataaattggc ttcttcaaat tcgacttggg cacttcgact gtgacttcgt
     3721 aaaatcgccc agtccggtcg tcacccagtg cgtccgcgct gaccaccgcc acgcggccgt
     3781 cgagcgtatc ggcataacta ttgccgaagg cattcacatg cactcgcgcc ggcatgcctt
     3841 gccagatctt gtcgcgatct tccggccgga tacgcacctg cgccacgagc ggcacatccg
     3901 tcggcgcaat ctcgaaaagg tcgccccccg gccggacgac agcaccggca tgcggattca
     3961 ccacgcgctg gatcacgccg tccgcgggac tttcaatctg tgcctgtttg cgccgatcct
     4021 gactcgcatt cgccttggcg ttcacttgcg cgagttcgtc gcgcgcctgc gaaagctgct
     4081 tctgcgcatc ggacatgaag tcggttttga tctgttcaac cttgagccga tattcgcgct
     4141 cttcggcgac aagccgtggg accttcgcgc gcgcctcgtt caacgcactc tgcgcgcttt
     4201 gcagatcgga acgtttctgt aacagcacgc cttcggcagc ggcccctttg cgcacaagag
     4261 gcgcgatcat gtcgacctgt tgctgcatga gcgcaacctc cttgtcgagg tcgccgatac
     4321 gggtgcgcag ctcagccgat tgcgcgagcc gctgatccgc ctgaacctgc gaaatcgaag
     4381 ccgactggtc aagggcgagc tggcgcgact catacagcgc cttttctccc gcgacggcct
     4441 tcgcctgttc agcgtcggca ggcattgctg gatcgaaccg tgtgtgtgcg agctcggcct
     4501 cgagtctctg ttcgcgaagg cgcagcgtgc tgagcgctgc ggaattcctg gcgaattcct
     4561 cggacacata ggtgttatcc atcacagcaa gcaactgtcc ctttcggacc gtttgccctt
     4621 cgcgcaccag aagcttttcg atctgtccgc cgtcagcgtg ttgcacgacg atattgtggc
     4681 tctccacgcg gaccacacca gggccgcgaa cgagcacatc gagcttgaac atggtcgacc
     4741 acacagctag tagcgtaaag ccaagcagcg ccacgccggt catgccgcac ggcgcataag
     4801 ggcggcacag cgcgatgccc tgtctgattc tgataacgat gaggctaaat ttttctttca
     4861 tgacagtcac agcgcgacac cgggtgcctc gctgtatcga gagtgggaaa cgcactacct
     4921 gaggcgaaga acgatcgagt cttgcgtgcc atcgtccggc acgggattgc gcaaatcgat
     4981 cttgtcacgc gggatgccga gttcatcggc aatcgccacg accatcagcg agcgctcgta
     5041 ttgcaggcgc atacggacac caagaggcgc gtcagtcgca ccggtgagtg agaacacaag
     5101 caagccgcgc tgccggattg cctgagcgtg gctgctcatc cagccgcgca cggccgcccg
     5161 ggcggtgggc tgcaggaagt tttcgtcgcg tcccacgctg gcgacgaaat cgtcgggctg
     5221 ccgcgtcagc ttgtcggtca gttcgactgc gcctgccggc gagctcgatt cgctcgaact
     5281 ggtggctggc gcactctcga caggaggtcc ggccagcgcc gccaacacct gaaaaatcat
     5341 gctaagcagc aggtacacca tcagcagcac cgtcatcatg tcgacgtacg ctggccagat
     5401 atggttcttc gatccggatg ccattacgct tcctccttga tcagattgag tgcgcgcaac
     5461 ccgtccatgg ttttcgcgtc catgttgtag agcgccagcg tcgccaggta atagccggat
     5521 tcagcttgtt cgcggtcgct gcgcgcagag aacaaatcga cttgggcgtt gatcaggtcg
     5581 gacagttgac gcgtgccaag gcggaattca tccgcgtaca gctcgagcgt gcgcagtgcc
     5641 tgcttctcgc cgtcgacact cagcgtccac tgatcacgtg cactgtccca atcagagcgc
     5701 agcgcctcgg tttgatcgtt gacattgcgc agctcctgtt caagctgttg ctgcgcaacg
     5761 tatttcgccg atacggcctc gttcacacga gacttctgca gaccgccgtc gaacagatcg
     5821 aaggtcacct gaagcatcac tgaactgcca ggcgagggaa cgttctgaaa tggtttgcga
     5881 acggtggcta cgatgtcgac gcgcggataa cgcgacgcgc gctcgacgtt gaccaacgcg
     5941 atattgctct caacattcgc gcgacgcgcc ttgactgcca ggttcgcgtc cgcagccgat
     6001 tcgatcaacg gttgaaggtt gaccggagga agttctttca cgcggtccct ctgctgcggt
     6061 accgcatcca gaccggtgat cgacttgaac tgttgcttcg ccttcgtcag ttgcaaattg
     6121 atctgcttgc cgcgagccta cgcggaagtc agcgccagtt ggccccggat cgcatcggat
     6181 tgcggcgcca ggccaccggc tacgcgctcc tcgattaccg aattcagacg ctgcacctga
     6241 gcgacgtagt cctcagcgat ttttttcaga tgctgatagc gcacaacgtc gagataggcg
     6301 cgtgaggtgc tataggcgag catttcaacc tcgccttgat acgcgctttc cgcggcctcg
     6361 gcggagagcg ccgcactact cacctggttc ttccggcgcc cgaaatcgaa caccgtgtac
     6421 tggatcgtgc cgtttacctg cgaatattga tcaaaggttc ggccgtagcc ttgcgtcgcc
     6481 ccataaatcc tgtccggcgt cttggctatt acgcccacct tcgggtagta agcggcgcgc
     6541 gcaccgctga cacgcgcctg cgcctcgtca gcctgaccgc gtgcgatttt tgtcg
//
LOCUS       DATASH010001013         5559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3340843,
            whole genome shotgun sequence.
ACCESSION   DATASH010001013 DATASH010000000
VERSION     DATASH010001013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5559
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3340843"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="VHV99_26930"
     CDS             complement(<1..603)
                     /locus_tag="VHV99_26930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004525857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX3639053.1"
                     /translation="MQFDKRAWLDRIKRMFLGFDRPLALIVFLLLCVGIVTLYSATLD
                     MPGRVEDQMRNIMLTFVLMWALANVPPTTLMRFAVPLYTFGIALLIAVAVFGLTRKGA
                     KRWINVGVVIQPSEILKIATPLMLAWYYQRREGVMRWYDFLVGFLILIVPVGLIAKQP
                     DLGTAVLVFAAGFFVIYFAGLSFKLIVPVLIAGVIAVGSIA"
     gene            complement(614..2986)
                     /gene="mrdA"
                     /locus_tag="VHV99_26935"
     CDS             complement(614..2986)
                     /gene="mrdA"
                     /locus_tag="VHV99_26935"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX3639054.1"
                     /translation="MTEFKDTQQQLSKFRLRVAAAGLFVFVCFGLIGFRFLVLQVWHY
                     SKYSLQADENRISVAPIVPNRGIITDRNGVVLAKNYSAYTLEITPSKLNDTLENVIDN
                     LATVVSIDARDRRRFKKLQEDSKNFESLPIRTRLTDDEVARFTAQRFRFPGVEVRARL
                     FRQYPLGPTAAHVIGYIGRISQRDQDRIDDASDQNDSDPDHYDPRLDANNYKGTDYIG
                     KIGVEQSYETELHGQTGFEEVEVTAGGRPVRTLSRTQATPGNNLELSLDIGLQQVAEQ
                     AFAGRRGALVAIEPATGDVLAFVSAPSFDPNSFVDGIDQQTWDDLNNSPDHPLLNRPL
                     HGTYPPGSTYKPFMALAALTLHKRTPGWGFQDPGSYTFGGHTFRNDVRSGQGWVDMNR
                     AIVVSNDTYFYMLAHDLGVNAIAGFMKPWGFGQITGIDISGEARGILPSTEWKRKAYR
                     KPEQQRWYEGETISLGIGQGYNSYTILQLAHATATLANNGVVMKPHLVRDIENPITKE
                     MRPVVRDPSDRIAVKQSDIDIIKRAMEGVVTNGTAAKVFIGAPYQAAAKTGTAQVYSL
                     QGANYKGHAIAEHLRDHALFIAFAPAEQPKIAIALIVENGGWGAEAAGPIARRVLDYY
                     LVGKNKPGAEAAAVAAAASATQDASAPVVGGAPAPQDAAQPVKVAAGFTALPIPGAAS
                     AAASASAAAAASAPAAASTAPAAASTPGATASTRAPATAAPASGASAAVSALASASAA
                     AKNPTPRKSAVPHKPRPSSEPAPTAAAAPSRDDSIQPTSPRQPVSGGIDE"
     gene            complement(3129..3641)
                     /gene="mreD"
                     /locus_tag="VHV99_26940"
     CDS             complement(3129..3641)
                     /gene="mreD"
                     /locus_tag="VHV99_26940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEX3639055.1"
                     /translation="MNRPQYILQPVNPYFIAFSLAAAFLLNLMPWGRLAGVPDFVALV
                     LLFWNVHQPRKVGMGIAFFLGLLMDVHNAGLLGEHALAYTLLSYGAITIHRRVLWMSL
                     GVQTFAVMPLLVVAQLVPFVIRLLTGAAFPGWGYLVDGFVEAALWPVASMLLLMPQRR
                     PADPDDTRPI"
     gene            complement(3638..4777)
                     /gene="mreC"
                     /locus_tag="VHV99_26945"
     CDS             complement(3638..4777)
                     /gene="mreC"
                     /locus_tag="VHV99_26945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEX3639056.1"
                     /translation="MEYSPPPLFKQGPSALARLVFFVVLALALLISDARFNTLEIVRG
                     VLGAGLYPLQRAALVPRDIFMGAADLAVTSATLRSENDKLRTKDLQLAQQANTAAELA
                     AENTHLRALLQLSQRSTTQSLPAEIQYDTRDPFTQKVVIGRGAQQGVQNGSPVVNEDG
                     VIGQVTRVFPLQAEVTLLTDKDQAVPVQIVRTGLRSVIYGTPKGDTLDLRFVPISADV
                     QTGDELVTSGLDGVYPPGLPVAKVVRVDKQADTAFARVICLPVAPVRGARQLLVLHYL
                     NNVPPRPAEEPDAATTAKDAKAKKGGKPADSKAAADKSPARAAENPAAGAKPADQPAA
                     KPAEKPSAKAPATEKKAATEKKAAAEKKPAADKTAKPQATPGAQP"
     gene            complement(4931..>5559)
                     /gene="mreB"
                     /locus_tag="VHV99_26950"
     CDS             complement(4931..>5559)
                     /gene="mreB"
                     /locus_tag="VHV99_26950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HEX3639057.1"
                     /translation="YLIEEPMAAAIGAGLPVSEATGSMVVDIGGGTTEVGVISLGGIV
                     YKGSVRVGGDKFDEAIVNYIRRNYGMLIGEQTAEAIKKEIGSAFPGSEVKEMEVKGRN
                     LSEGIPRSFTISSNEILEALTDPLNQIVSSVKIALEQTPPELGADIAERGMMLTGGGA
                     LLRDLDRLLAEETGLPVLVAEDPLTCVVRGSGMALERMDKLGSIFSYE"
BASE COUNT          931 a         1746 c         1879 g         1003 t
ORIGIN      
        1 cgcgatcgag ccgaccgcga tcacgccggc aatcagcacc ggcacgatca gcttgaaact
       61 cagccccgcg aagtagatca cgaagaagcc ggccgcgaac accagcacgg cagtgccgag
      121 gtcaggctgc ttggcgatca aaccgaccgg cacgatcagg atcagaaagc cgaccagaaa
      181 gtcgtaccag cgcatcacgc cttcgcggcg ctggtagtac caggcaagca tcagcggtgt
      241 ggcgatcttc agaatttccg acggttggat caccacgccg acgttgatcc agcgcttcgc
      301 gcccttgcgc gtgaggccga acaccgccac cgcaatcagt aacgcgatcc cgaacgtata
      361 gagcgggacc gcgaagcgca ttagcgtggt gggcggcacg ttggccagcg cccacatcag
      421 gacgaacgtc agcatgatgt tgcgcatctg gtcttccacc cggccgggca tgtccagcgt
      481 cgcgctgtat agcgtgacga tgccgacgca cagcagcaga aacacgatca gggcgagagg
      541 acgatcgaag cccaggaaca tccgcttgat gcggtcgagc caggcgcgct tgtcgaattg
      601 catgcctttc tccttactcg tcgatgccgc cggaaaccgg ctggcgtggc gacgtaggct
      661 gaatgctgtc atcccgcgac ggggcggcgg cggcggtcgg tgccggttcg ctgctcgggc
      721 ggggtttgtg cgggacggcg gatttacgcg gcgtcggatt ctttgccgcg gcggacgcgg
      781 acgccaatgc ggagacggcc gccgacgcgc cggaggcggg cgcagcagta gccggcgcac
      841 gggtcgatgc agtggccccg ggcgtcgatg cagcggccgg agccgtcgat gcagccgccg
      901 gagccgacgc ggccgcagcg gcggacgctg acgctgcggc tgaggcggcg cccggtatcg
      961 gcagtgccgt gaaacccgcc gcgaccttga ccggctgtgc cgcgtcctgc ggcgccggtg
     1021 cgccgcctac tacaggcgcg gatgcgtcct gcgtcgccga ggcggccgcc gcgaccgctg
     1081 ccgcttcagc gccaggcttg ttcttgccga ccaggtagta atcgagcacg cgacgcgcga
     1141 tcgggcccgc cgcttcagca ccccagccgc cgttttcgac gatcaatgca atcgcgatct
     1201 tgggctgttc ggcaggcgcg aacgcgatga acagcgcgtg gtcgcgcaga tgttcggcga
     1261 tcgcgtggcc tttgtagttt gcgccctgca acgaatacac ctgtgccgtg ccggtcttgg
     1321 cggccgcctg atacggcgcc ccgatgaaca ccttcgccgc cgtgccgttg gtcacgacgc
     1381 cttccatcgc gcgtttgata atgtcgatgt ccgattgctt cacggcgatc ctgtcgctcg
     1441 gatcgcgcac gaccgggcgc atttctttcg taatcgggtt ttcgatgtcg cgcacgaggt
     1501 gcggcttcat cacgacgccg ttgttcgcga gcgtggccgt ggcgtgggcg agttgcagaa
     1561 tggtgtacga gttatagccc tggccgatgc ccagactgat cgtttcgcct tcataccagc
     1621 gctgctgctc gggcttgcgg tacgccttgc gcttccattc cgtcgacggc aggatgccgc
     1681 gcgcctcacc cgaaatgtcg atgccggtga tctggccgaa gccccacggc ttcatgaagc
     1741 cggcgatcgc gttgacgccg agatcgtgcg cgagcatgta gaaatacgtg tcgtttgaga
     1801 caacgatcgc gcggttcatg tcgacccagc cctggcccga gcgcacgtcg ttgcggaacg
     1861 tatggccgcc gaatgtgtac gagcccggat cctgaaagcc ccagcccggc gtgcgcttat
     1921 gcagcgtcag cgcggcgagc gccatgaacg gcttgtaggt cgaacccggc ggataggtgc
     1981 cgtgcagcgg gcggttcagg agcggatggt cgggcgagtt gttgagatcg tcccaggtct
     2041 gctgatcgat gccgtcgaca aacgagttcg gatcgaagct cggtgccgac acgaacgcga
     2101 gcacgtcgcc ggttgccggt tcgattgcga ccagcgcccc gcggcggccg gcaaaggcct
     2161 gctcggcaac ctgctgcagg ccgatgtcga gcgacagttc gaggttgttg cccggcgttg
     2221 cctgggtgcg cgacagcgtg cgcaccggcc ggccaccggc ggttacttcg acctcctcaa
     2281 aaccggtctg gccgtgcagt tccgtctcgt agctctgctc gacgccgatc ttgccgatgt
     2341 agtcggtgcc cttgtagttg ttcgcgtcga ggcgcggatc gtagtgatcc ggatcgctgt
     2401 cgttctgatc gctggcgtcg tcgatacggt cctgatcgcg ctgcgaaatc cggccgatgt
     2461 agccgatcac gtgcgccgcg gtcggtccga gcggatattg gcggaacagg cgcgcgcgca
     2521 cttccacgcc cggaaagcgg aagcgttgcg cggtgaagcg cgcgacttcg tcgtcggtga
     2581 ggcgggtgcg gatcggcagg ctttcgaagt tcttcgagtc ttcctgcagc ttcttgaagc
     2641 ggcggcggtc gcgggcgtcg atcgagacga cggtggccag attgtcgatc acgttttcga
     2701 gcgtgtcgtt cagcttcgac ggcgtgattt cgagcgtgta cgccgagtag ttcttggcca
     2761 gcaccacacc gttgcggtcg gtgatgatgc cgcggttcgg cacgatcggc gcaacggaga
     2821 tacggttttc gtcggcttgc agcgagtact tgctgtagtg ccagacctgc aggaccagga
     2881 aacggaagcc gatcagccca aagcagacga acacgaacag gcccgccgcc gcgacgcgca
     2941 ggcggaactt cgagagctgt tgctgagtgt ccttgaattc ggtcatgcga ttctgcaaaa
     3001 ggccgcgtga cggccatcat gagtgtgcgc ccaaaccggc cggaaccggg aggcatgaac
     3061 agcgatgcgc gtcgagcggg ccgggcgcct cagttccagc ggaacggccg gcgggctcga
     3121 atgcgtttct aaatcggccg cgtatcgtcc ggatcggccg ggcggcgctg cggcatcaac
     3181 agcagcatgc tggcgaccgg ccacaacgcg gcttcgacaa agccgtcaac cagataaccc
     3241 cagcctggaa acgccgcgcc cgtcagcaga cggatcacga acggcaccag ttgcgcgacg
     3301 accagcagtg gcatcaccgc gaaagtctgc acgccgagcg acatccacag cacccggcga
     3361 tggatcgtaa tcgcgccgta cgacaacaac gtatacgcca gcgcatgctc gccgagcaga
     3421 ccggcgttgt gcacgtccat cagcaagcca agaaagaagg cgatgcccat gccgactttg
     3481 cgcggctggt gcacgttcca gaacagcagc acgagcgcca cgaagtccgg cacgccggcg
     3541 aggcgccccc acggcatcag gttcagcaga aacgccgcgg cgaggctgaa cgcgatgaag
     3601 tacggattga ccggctgcag aatgtattgc ggacggttca tggctgggcc cccggcgtgg
     3661 cttgtggctt cgcggtcttg tcagcggccg gtttcttttc ggcggcggct ttcttctcag
     3721 tggcagcttt cttttccgtg gcaggggctt tggccgacgg cttttcagcg ggcttggcgg
     3781 cgggctgatc agccggcttg gcaccagcgg cggggttttc agcggctctg gcaggggact
     3841 tatccgccgc ggccttgctg tcggcgggct tgccgccctt cttcgctttc gcgtccttgg
     3901 cggtcgtggc cgcatcgggc tcttctgctg ggcgcggcgg cacgttgttc agatagtgca
     3961 gcaccagcaa ctgacgcgcg ccgcgcacgg gagcaaccgg caggcagatc acgcgcgcga
     4021 atgccgtatc ggcctgtttg tcgacccgca cgaccttggc gaccggcagc cccggcggat
     4081 aaacgccgtc gagtccgctg gtgaccagtt cgtcgccggt ttgcacgtcg gcgctgatcg
     4141 gcacgaagcg cagatcgagc gtatcgccct tcggcgtacc gtaaatcacg ctgcgcaagc
     4201 cggtgcgcac gatctgcacg ggcacggcct ggtccttgtc ggtgagcagc gtcacttcgg
     4261 cttgcagcgg gaacacgcgc gtgacctggc cgatcacgcc gtcttcattg accaccggcg
     4321 aaccgttctg cacgccttgc tgggcgccgc ggccgatcac gaccttctgt gtgaacggat
     4381 cgcgcgtgtc gtactggatt tcggccggca gcgattgcgt ggtcgagcgc tgcgagagtt
     4441 gcaacagcgc ccgcaaatgg gtgttttcgg ccgccagttc ggcggccgta ttggcttgct
     4501 gcgcgagctg gagatctttg gtgcgcagct tgtcgttctc gctgcgcagc gtggcgctgg
     4561 tcacggccag gtcggcggcg cccatgaaga tgtcgcgcgg cacgagggct gcgcgttgca
     4621 acggataaag acccgcgccg agcacgccgc gaacgatttc gagcgtattg aagcgtgcat
     4681 cggagatcag cagggcgagc gccagcacga cgaaaaacac cagtcgtgcg agggcggacg
     4741 ggccttgctt gaacaggggc ggcggactgt attccatggt cggcgccggg ggcgtgagcg
     4801 gttgtgtgag acggcggcgc gaaggacgcg cgttcagcgc gcggaggctg gcaggcccag
     4861 ccaacggcaa accggcccgc tagtggccgt ttgcctgatc cgcgctactg acatggagac
     4921 gggctctcgc ttactcgtac gagaagatgc tgcccagctt gtccatgcgt tcgagcgcca
     4981 tgcccgagcc gcgcacaacg caggtcagcg ggtcttcggc gacgagcacc ggcaggccgg
     5041 tttcttcggc gagcaggcgg tccaggtcgc gcagcagcgc gccaccgccc gtgagcatca
     5101 tgccgcgctc ggcaatgtcg gcgcccagtt ccggcggcgt ttgttcgagc gcgatcttca
     5161 ccgacgacac gatctggttc agcggatcgg taagggcttc gagaatttcg ttactggaga
     5221 tcgtgaagct gcgcggaatg ccttccgaca gattgcggcc cttcacttcc atttccttga
     5281 cctcggaacc cgggaaggcg gagccgattt ccttcttgat ggcttcggcg gtttgctcgc
     5341 cgatcagcat gccgtagttg cggcggatgt agttgacgat agcttcgtcg aacttgtcgc
     5401 cgcccacgcg caccgaaccc ttgtacacga tgccgcccag cgagatcacg ccgacttcgg
     5461 tcgtgccgcc gccgatgtcg acgaccatcg agccggttgc ttccgacacc ggcaggcctg
     5521 cgccgattgc cgctgccatg ggttcttcga tgaggtaga
//
LOCUS       DATASH010001014         1691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6108184,
            whole genome shotgun sequence.
ACCESSION   DATASH010001014 DATASH010000000
VERSION     DATASH010001014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6108184"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(308..1429)
                     /gene="asd"
                     /locus_tag="VHV99_26955"
     CDS             complement(308..1429)
                     /gene="asd"
                     /locus_tag="VHV99_26955"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012338485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX3639058.1"
                     /translation="MNVGLVGWRGMVGSVLMQRMQQEGDFDLIEPVFFSTSNAGGNAP
                     SFAKNETKLKDATSIEDLKKCEAIISCQGGDYTNEVFPKLRAAGWNGYWIDAASSLRM
                     KDDAVIVLDPVNLDVIKNALVKGQKNFIGGNCTVSLMLMALGGLFRENLVDWMTAMTY
                     QAASGAGAQNMRELLQQMGTLYGAAKEDLADPSSAILDIDRRVLSAMNSDRMPTDHFG
                     VPLAGSLIPWIDKDLGNGMSKEEWKGGAETNKILGKPAMGTPGSIPVDGLCVRIGAMR
                     CHSQALTIKLNKDVPLDEVNSILASGNDWVKVVPNEREASMRDLSPAVVTGTLTVPVG
                     RVRKLAMGGEYLSAFTVGDQLLWGAAEPLRRMLRIVLDK"
BASE COUNT          319 a          533 c          504 g          335 t
ORIGIN      
        1 aggcggcgtc cggcgcgctc gcggcatcgc cgggcgcggc gagcgcgctg cccatgccag
       61 cgccggccag cgccaaaacc aaagcggctg cggccgccgg gcggcccgtc aaacggtttt
      121 tatcttgatg attggacata gcccgaaggg atgagagtcg aaggttcatc gggactccag
      181 gcgcgtcagc gcgcagccct tgttcgcggt tcgaaagtga caagatgatc ggagcacaca
      241 atgaaaaaag cgccgcgcaa gcgcgacgct tcctggtttt ctacgcgtcg tgagcgcgta
      301 gtttacttca cttgtcgagt acaatgcgaa gcatgcgacg cagcggttcg gccgcgcccc
      361 acagcagctg atcgcccacg gtgaaggccg acagatattc gccgcccatc gccagcttgc
      421 gcacccggcc caccggcacc gtcagcgtgc ccgtgaccac cgctggggac aaatcgcgca
      481 tcgacgcttc gcgctcgttc ggcacgacct tgacccagtc gttgccggac gcgaggatgc
      541 tgttcacctc gtcgagcggc acatccttgt tcagcttgat ggtcagcgcc tgcgagtggc
      601 agcgcatcgc gccgatgcgc acgcacagac cgtcgaccgg gatcgaaccc ggcgtgccca
      661 tggccggctt gccgagaatc ttgttggttt cggcaccgcc cttccactct tccttcgaca
      721 tgccgttgcc gagatccttg tcgatccacg gaatcaacga gccggcgagc ggcacgccga
      781 aatggtcggt cggcatgcga tcgctgttca tcgcgctcaa cacgcgacgg tcgatgtcga
      841 gaatcgccga cgacggatca gccaggtctt ccttggccgc accatacagc gtgcccattt
      901 gctgcagcaa ctcgcgcatg ttctgcgcgc ccgcgcccga agcggcctga tacgtcatgg
      961 ccgtcatcca gtcgacgagg ttttcgcgga acaggccgcc gagcgccatt agcatcaggc
     1021 tgaccgtgca gttgccgccg atgaaattct tctgcccctt gaccaacgcg ttcttgatca
     1081 cgtcgagatt gaccggatcg agaacgatga ccgcgtcatc tttcatgcgc agcgacgagg
     1141 ccgcgtcgat ccagtagccg ttccagcccg ccgcgcgcag cttcgggaag acttcgttcg
     1201 tatagtcgcc gccttggcag gagatgattg cttcgcactt cttcaggtct tcaatgctgg
     1261 tcgcatcttt gagcttggtc tcgtttttgg cgaacgacgg cgcgttgccg cccgcgttgc
     1321 tggtgctgaa aaacaccggt tcgatcaagt cgaaatcgcc ttcctgctgc atgcgttgca
     1381 tcaggacgct gccgaccatg ccgcgccaac ctacgagacc tacgttcatg acttcatacc
     1441 cttcgaatgg aaacttcccc gcatctttgc ccgcagtgac cgcgcgggga agatgggcgg
     1501 gcacgacgga cgatcagcgc ttggtgatcg ttttcggggt aatggttttg atggtaatgg
     1561 cgaactcgat cgcacgggcc gttgtgccgt gcgatttgaa aatcgaggag atttgggaga
     1621 tctgagccat cgcaacaata tacacgaaaa cggaaaactg gcggcgctcc tcgccgtatt
     1681 gagcacgaat c
//
LOCUS       DATASH010001015         2196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4127494,
            whole genome shotgun sequence.
ACCESSION   DATASH010001015 DATASH010000000
VERSION     DATASH010001015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4127494"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="VHV99_26960"
     CDS             <1..967
                     /locus_tag="VHV99_26960"
                     /inference="COORDINATES: protein motif:HMM:NF015532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OprD family outer membrane porin"
                     /protein_id="HEX3639059.1"
                     /translation="TLAQAALKWRSTATGDGWQVRAGYTPISVGTLGTSGGLHSHAYR
                     GVELRYRVAGWEVGYGLADQFRNEWDNRFRPMTNAWHQNRDQYDGTASRIDFVHSVGV
                     RYEANADSSVDAGVGEGRRYRRNAQVAATRAWRIADSGTMTATGYALWGRYEAALGPT
                     ATPRNEWHASTSVAYATGPFTVSLGFGATHAPDSGEMNFRLTPWANSDNRNQIQTWGQ
                     LDDFVWDGTRVLKAAASYQIGSYIGVPGLAVGISGIQGWAMKNPGRGTTSANEIDLSM
                     TYDVKEGPLKGVGLGVFPARLRTNGFYGKSDRNDVKIIASYSKTF"
     gene            980..>2196
                     /locus_tag="VHV99_26965"
     CDS             980..>2196
                     /locus_tag="VHV99_26965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011353252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydate-binding domain-containing protein"
                     /protein_id="HEX3639060.1"
                     /translation="MNLNLSRIALMAGLLAMVATLPAFALSPVPPVPLSAPPSADATL
                     TAVLASDLMLRVSIDNNDAAKAGVPCAGLGADSDSCATGRLILVNEGLTRITGDGWKL
                     YLHSIRRLLKIDHPDFSLRHLTGDLYELTPRFGSVSLGAGQRLEIPFVAEYWLLRYSD
                     VLPRPYVVVAGEQPAVLKHNDTDDETRYVDRLPGGVPTIPTTPAQKLRQMVERGEPLP
                     AKQVAARALPSVMRQQAGVGEVELRGVELSLPDLPPASVAALREHAATLGLAKGRLRI
                     TGALMPNGLPADIAVPGGYRLRIARTGVMVDGYDTAGLYYGVQTLLSLAPAGGGRIPA
                     MTIEDAPRFGHRGMHVDVARNYRQPATLRRLIDQMSAYKLNRLHLHLTDDEGWRIEIP
                     GLPELTAIGAQRC"
BASE COUNT          399 a          683 c          719 g          395 t
ORIGIN      
        1 cacgctcgcg caagctgcgc tgaagtggcg tagcacggcg accggcgacg gatggcaggt
       61 gcgcgccggc tatacgccca tcagcgttgg cacgcttgga acgtcgggtg gcctgcattc
      121 gcacgcgtac cgcggcgtgg agttgaggta ccgcgtggca ggctgggagg ttggctacgg
      181 gctggctgat cagtttcgca acgaatggga caaccggttt cggccgatga ccaacgcgtg
      241 gcatcagaat cgcgaccagt acgacggcac ggccagccgt atcgacttcg tgcatagcgt
      301 cggcgttcgc tacgaggcga atgccgatag ctccgtcgac gcgggcgtgg gcgagggcag
      361 gcggtatcgc cgaaatgcgc aggttgccgc cacgcgggcc tggcgtatcg ccgacagcgg
      421 cacgatgacc gcgacgggct acgcgctgtg gggccgctat gaagcggcgc tggggccgac
      481 cgccacgccg cgcaatgaat ggcacgcgag caccagcgtg gcctatgcca ccggaccctt
      541 cacggtcagt ctgggctttg gagccacgca cgcgcctgac tccggcgaga tgaattttcg
      601 cctcacgcca tgggcgaaca gcgacaaccg caaccagatc cagacctggg gccagctcga
      661 cgacttcgtc tgggacggca cgcgggtgct caaggcagcg gcttcctatc agatcggctc
      721 gtatatcggc gtgccgggtc ttgcggttgg catcagcggc attcaggggt gggcgatgaa
      781 aaatccgggg cgcggcacga cctcggcgaa cgagatcgac ctgagcatga cttacgacgt
      841 gaaggaaggc cctctcaagg gcgttggcct tggcgttttc cccgcgcgtc tgcgtaccaa
      901 cggcttctat ggaaagtctg atcgcaacga cgtgaagatc atcgcgtcgt acagcaagac
      961 cttttgaagg gatcacatca tgaatctgaa tctgagtcga atcgccttga tggccggatt
     1021 gctggcaatg gtggccacgc tgcctgcatt tgccttgtct cctgttccgc ctgttcctct
     1081 ttctgcacca ccgtccgccg acgcgacgtt aaccgccgtg ctcgcgagcg atctcatgct
     1141 gcgcgtgtca atcgataaca acgacgcggc gaaagcaggc gtgccgtgtg ccggcctcgg
     1201 cgcggattcg gacagttgcg caaccggtcg cctgatactg gtgaacgaag ggctgacgcg
     1261 aatcacgggc gacggctgga agctgtatct gcacagcatc cgccgtctcc tgaagatcga
     1321 tcatcccgat ttctcattgc ggcacttgac gggcgacctc tacgaactga ccccgcggtt
     1381 cggttcagtg tcgcttggcg cgggtcagcg ccttgaaatt ccgttcgtcg cagaatactg
     1441 gctgttgcgt tacagcgatg ttttgccccg gccctacgtt gtcgtggcgg gcgagcagcc
     1501 ggctgtgctc aaacacaacg acacggacga cgagacgcgt tacgtcgacc ggctgccggg
     1561 tggcgtaccc accatcccca caacgcccgc gcagaagctg cggcaaatgg tcgaacgcgg
     1621 cgagccgctc ccggccaagc aggttgccgc acgcgctttg ccgtcagtga tgcgtcagca
     1681 ggccggtgtc ggcgaagtcg aattgcgggg cgtcgagttg tccttaccag atttgccgcc
     1741 ggcaagcgtc gccgcgctgc gtgaacatgc cgctacgctt ggcctcgcga agggtagatt
     1801 gcgaatcacc ggggcgctca tgccgaacgg cttacctgcc gacatcgccg tgccgggcgg
     1861 ataccggttg cgaatcgcgc gcaccggcgt gatggtcgac gggtacgaca cagcgggcct
     1921 ctactacggc gtgcagaccc tgttgtcgct cgcgcctgcc ggaggcggcc ggattcccgc
     1981 catgaccatt gaggacgcac cgcgtttcgg gcatcgcgga atgcacgtgg atgtcgctcg
     2041 caactaccgg cagcccgcga cgctgcgccg tctcatcgac cagatgagcg cctacaagct
     2101 gaaccgctta catctgcatt taaccgatga cgaaggctgg cggatcgaga taccgggact
     2161 gccggaactg acggcgatcg gcgcacaacg atgtca
//
LOCUS       DATASH010001016         4302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4328534,
            whole genome shotgun sequence.
ACCESSION   DATASH010001016 DATASH010000000
VERSION     DATASH010001016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4302
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4328534"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            225..1418
                     /locus_tag="VHV99_26970"
     CDS             225..1418
                     /locus_tag="VHV99_26970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018435552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HEX3639061.1"
                     /translation="MEGFITDWLNLAIRWFHVIAAIAWIGESFYFVALDNSLKPPEDP
                     NQRRRGVFGELWHVHGGGFYNMQKYTVAPPEMPDDLHWSKWPSYTTWLSGFGLFTVLY
                     LFSPSTYLIDRNVLDMGPVVAVASALGFLAAGWIVYDSLCRLLGNKDKVLGICVGAYV
                     LIAAWLACHIFAGRAAYLIMGAMLATIMSANVFFVIIPGQRKMVASMLKGETPNPIYG
                     KRGKQRSVHNTYFTLPVVFAMLSNHYAMTYTHPYNWAVLVIIMLAGALIRQFFVMRHR
                     GQVLWYLPLVGIVLMFCALFWTMPKPIVPQAQAANAPVVRMTDIAPVLQQRCVACHSA
                     HPTMMGSAPAGVLLDTPDEISQNAQRIYQQAVTLKAMPLGNVTHMTDDERMKIAAWFE
                     GGAAK"
     gene            1687..1953
                     /locus_tag="VHV99_26975"
     CDS             1687..1953
                     /locus_tag="VHV99_26975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX3639062.1"
                     /translation="MTNLADVADMLKAVRRDSNLSQEELAHRAGVARTTVARMETLAR
                     NDMSVSALVRLLEAAGYDLKFVPHGHGRTLEDILAEQRMPDSNS"
     gene            1950..3302
                     /locus_tag="VHV99_26980"
     CDS             1950..3302
                     /locus_tag="VHV99_26980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020423664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HEX3639063.1"
                     /translation="MRLDVQVLGKNVATLFRERDDYILKYNRDATANDFVSLTMPVRE
                     EAWRWPRDLHPFFRQNLPEGYLLNLIREQFGPLLDGTDLSLLAVVGAMGIGRVTVTPE
                     GVEPGTALQPLDVQDILHGDNTAEHFAVLVREYARAAISGAVPKFIAPQAQIAASRST
                     PMPLGKPTIRTSRHIVKGSDDNTPFLGFNEFYSMRVLEHVGVAPVARTQMSDDGRALI
                     VERFDVDANGLPAYGVEDMCGLLGLPPHEKYNSTSEKMLNAAKAYLLDRDSMRLQLEQ
                     LGWHLLTNYVVRNADCHTKNVALFYTSVDDVTFTPVYDIVTTQAYPRFAANPPGLPIN
                     GRKTWAAGKALERFFNTRMGIAPRQYAQMVEALCDSAVEVGHELIEASRNEPQWRQVA
                     KQMLHAWDEGMTSLRSPKKSPQFKGLKPAIEAAGFSAPEPAERAREIIGRSPLLGKRG
                     "
     gene            complement(3422..3934)
                     /locus_tag="VHV99_26985"
     CDS             complement(3422..3934)
                     /locus_tag="VHV99_26985"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HEX3639064.1"
                     /translation="MKTLAIEPLTKEAFAAFGDVIELEGAKQIPINLGTTIRYHDLAK
                     VDVTDEDGRTLVNLFRGQPRTLPFEVKMLERHPLGSQAFVPLNDKPYLVVVAPAGELD
                     PRQIRAFVTSGWQGVNYAKGVWHHPLIALGDVSDFIVVDRGGDGLNLNEQDLAESLWL
                     TEDALSAVTV"
     gene            complement(3931..>4302)
                     /locus_tag="VHV99_26990"
     CDS             complement(3931..>4302)
                     /locus_tag="VHV99_26990"
                     /EC_number="3.5.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488640.1"
                     /note="catalyzes the formation of S-ureidoglycolate and
                     urea from allantoate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoicase"
                     /protein_id="HEX3639065.1"
                     /translation="RGANMGDGWETRRRREPGNDWAIVALAQPGVITKIEVDTAHFKG
                     NYPDRCSIQAAYVTGGTDSSLITQAMFWPVLLDEQKLKMDNQHYFESEIAALGPVTHV
                     RFNIIPDGGVSRLRLWGTLAS"
BASE COUNT          859 a         1299 c         1283 g          861 t
ORIGIN      
        1 cgcaatcgaa tgcgagcgac tcgaccccag gttccaataa aaaacggcgc cgtgcgaact
       61 gagcgcaccg cgtctttcgc cctgaaagaa aggccccttg ggggacaccc cgcagtctgt
      121 gcagcggcgc catgcatgcc gacgacccgt cagccgtgtc accgaaggcg cagtgaggac
      181 accagcgtcg ttgcacgcac aacgaatcag aagtggagga gttcatggaa ggctttatta
      241 ccgactggct gaatctggcg attcgctggt tccacgtcat cgccgcgatt gcatggatcg
      301 gcgaatcgtt ttacttcgtc gcgctggaca acagcctgaa accaccagag gaccctaacc
      361 agcgcaggcg cggagtgttc ggcgaactgt ggcatgtgca cggcggcggc ttctacaaca
      421 tgcagaagta caccgtcgcg ccgccggaaa tgccggacga tctgcactgg tccaagtggc
      481 cttcgtacac cacgtggctg tcgggcttcg gcctcttcac ggtgctgtat ctgttctcgc
      541 cgtccacgta cctgatcgac aggaatgtgc tcgacatggg tccggtggtg gccgtcgctt
      601 cggcgctcgg cttcctcgcc gcgggctgga tcgtgtatga ctcgctgtgc cgtttgctgg
      661 gtaacaagga caaggtgctc ggcatctgtg tcggcgctta cgtactgatc gcggcgtggc
      721 tcgcgtgtca tatcttcgca ggccgtgcgg cgtacctgat catgggtgcg atgctggcca
      781 cgatcatgtc ggcgaacgtg ttcttcgtga tcatcccggg tcagcgcaag atggttgcct
      841 cgatgctcaa gggtgaaaca ccgaacccga tctacggcaa gcgcggcaag caacgttcgg
      901 tgcacaacac gtatttcacg ctgccggtgg tgttcgcgat gctgtcgaac cactacgcga
      961 tgacctacac gcatccgtac aactgggccg tgctcgtgat catcatgctg gccggcgcgc
     1021 tgattcgcca attcttcgtg atgcgtcacc gtggccaggt actgtggtat ttgccgctgg
     1081 tcggtatcgt gctgatgttc tgcgccctct tctggacgat gcccaagccg atcgtgccgc
     1141 aagcacaggc ggcgaatgcg ccggtcgttc gaatgaccga tatcgcgccg gtgctgcaac
     1201 agcgctgcgt ggcatgccac tcagcgcatc cgacgatgat gggcagcgcc ccggccggcg
     1261 tgctgctgga tacgccggat gagatctcgc agaacgcaca gcggatctat cagcaggcgg
     1321 tgaccttgaa ggcaatgccg ctgggcaacg tcacgcacat gaccgatgac gagcgcatga
     1381 agatcgccgc gtggttcgaa ggcggtgcag cgaagtaatg gaaatggccg ggcatcgcgt
     1441 ccggctctgc ggattggaaa gcgggctttt tcgcgcaagc gggaagcccg ttttttttcg
     1501 ttggcggcgg gcaggatagg tcagttgcac ggcacaacct gaaaaaaatt tgcagcccaa
     1561 gggctacaat ttagactaca ttcttctgtt ttgtagccta cgggatacga atctactgaa
     1621 acacattgtg tcccataggc tacaatatag cctgttttcc gccaaattga tgcccttggg
     1681 ctacaaatga ccaatctcgc tgacgtcgca gacatgctga aggccgttag gcgcgacagc
     1741 aacttgtcgc aggaggaact ggcgcaccgt gcgggggttg cccgcaccac ggtcgcgcgc
     1801 atggaaacgc tcgccaggaa cgatatgagc gtttcggccc ttgtgcgtct gctcgaggcg
     1861 gcaggctacg acctaaaatt cgttccccac ggacacggac ggacactcga ggacatcctg
     1921 gcggagcaac gcatgccgga ttccaactca tgaggctcga tgttcaggtt ctaggcaaaa
     1981 atgtcgccac gctgttccgc gagcgcgacg attacatcct caaatacaac cgtgatgcga
     2041 cggcgaacga cttcgtcagt ttgaccatgc cggtacgcga agaagcctgg cgctggcccc
     2101 gcgacctgca tccgtttttc cggcagaacc ttcccgaagg ctatctgctg aatctgatcc
     2161 gcgagcaatt cggccctctg ctcgacggca cggacctctc gttgcttgcg gtggtcggtg
     2221 caatggggat tggccgcgtc accgtgacgc ctgaaggcgt tgagcctggc accgccttgc
     2281 agccgctgga cgtgcaggac atcttgcacg gcgacaacac agccgagcat ttcgccgtac
     2341 ttgtgcgcga atatgcgcgc gcggccatct cgggcgccgt gccgaagttc atcgcgcctc
     2401 aggcgcaaat cgctgccagt cggtcaacgc caatgccgct cggcaaaccg accattcgaa
     2461 cgagccgtca catcgtcaaa ggctctgacg acaacacacc gtttctcggc ttcaacgagt
     2521 tttattcgat gcgcgtgctg gagcacgtcg gcgtggcgcc ggtcgcgcgc acgcagatgt
     2581 cggatgacgg ccgtgcgctg atcgtcgaac gtttcgacgt ggatgccaat ggactgcctg
     2641 cgtatggtgt cgaggatatg tgcggattgt tgggcttgcc gccacatgaa aaatacaatt
     2701 cgaccagcga gaagatgctc aatgccgcaa aggcgtatct gctcgatcgc gacagtatgc
     2761 ggctgcaact cgaacaactc ggctggcatc tgctgacgaa ctatgtggtg cgcaacgcag
     2821 actgtcatac gaagaacgtc gccctgttct acacgtcggt cgatgatgtt acgttcacac
     2881 cggtctacga catcgtcacc acacaggcct atccgcgttt cgcggccaat ccaccgggtt
     2941 tgcccatcaa cgggcgaaaa acctgggcgg ccggaaaagc gctcgagcgt tttttcaaca
     3001 cgcgtatggg gattgcgccg cggcagtacg cgcagatggt ggaagcgctg tgcgattcgg
     3061 cggtcgaggt cggtcatgaa ttgatcgaag cgtcgcgcaa tgaaccgcag tggcgccagg
     3121 tcgccaaaca gatgctgcat gcgtgggacg aaggcatgac gtccttgcga tcgccgaaga
     3181 aaagcccgca attcaaagga cttaaaccag cgatcgaagc ggcaggattt tccgcgcccg
     3241 agccggcgga gcgcgcgcgg gaaatcatcg ggcgttcacc gctactcggc aagcgcggct
     3301 gaaatacggc atcgcgccgg caacccgcct ctgcagagca aaaagcaaaa agcccacaac
     3361 atgcactgtg ggctttttgc atggtcggct tgctcgacct gctacatcga agcgcgaaat
     3421 ctcaaacagt caccgcgctc aacgcatctt ccgtgagcca gagcgactcg gccagatcct
     3481 gctcgttcag gttgagtcca tcgccgccgc gatcgacgac gataaagtcg ctcacgtcgc
     3541 ccaatgcgat cagcggatgg tgccaaacgc ccttcgcata gttcacaccc tgccagccgc
     3601 tcgtcacgaa cgcgcgaatc tgccgcgggt ccagttcgcc cgccggcgcc acgaccacca
     3661 gatacggttt gtcgttcagc ggcacgaagg cctggctgcc cagcggatgg cgttcaagca
     3721 tcttcacttc gaacggcagt gtgcgcggtt gcccgcgaaa cagattcacc agcgtgcggc
     3781 cgtcttcgtc ggtcacgtcc actttcgcga gatcgtgata gcggatcgtg gtgccgagat
     3841 tgatcggaat ctgcttcgcg ccttcgagtt cgatcacgtc gccgaatgcg gcgaacgctt
     3901 ccttcgtcaa cggttcgatt gccagcgttt tcatgatgcg agcgtgcccc acagacgcag
     3961 acgcgacacg ccgccgtccg gaatgatgtt gaaacgcacg tgcgtaaccg gacccaatgc
     4021 agcaatttcg ctttcgaaat agtgctggtt gtccatcttc agcttctgtt cgtccagcaa
     4081 caccggccag aacatggctt gcgtgatcag cgagctgtcc gtgccgcccg tcacgtacgc
     4141 ggcctggatc gagcaacggt ccggatagtt gcccttgaag tgcgccgtat cgacttcgat
     4201 ctttgtgatc acgcccggtt gtgcgagcgc gacgatcgcc cagtcgttac ccggttcgcg
     4261 gcgccgccgc gtttcccagc cgtcgcccat gttcgcgccg cg
//
LOCUS       DATASH010001017        11040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2942934,
            whole genome shotgun sequence.
ACCESSION   DATASH010001017 DATASH010000000
VERSION     DATASH010001017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 11040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11040
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2942934"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VHV99_26995"
     CDS             complement(<1..364)
                     /locus_tag="VHV99_26995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007747438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3639066.1"
                     /translation="MTLNTAPTVLIVPGLRDHVAEHWQTLLQARLSKTASVPPLEHDK
                     LSCAARVAALDQAIAKIDGPIVLVAHSAGVMITVHWAQRHNRPIQGALLATPADLEVP
                     MPAGYPALDVLEEHGWLPL"
     gene            complement(383..1231)
                     /locus_tag="VHV99_27000"
     CDS             complement(383..1231)
                     /locus_tag="VHV99_27000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX3639067.1"
                     /translation="MRVEQLNNAIGAELVGIRLADAIHDDGLFAEIRANLLRHRVLFL
                     RDQDISRAEHVAFARRFGELEDHPVAGSDPEHPGLVRIYKSPDQPNDRYENAWHSDAT
                     WREIPQFGAVLRCVECPPVGGDTMWANMVLAYENLPAHVKEQIADLRARHSIEASFGA
                     VMPIEKRHALKALYPDAEHPVVRTHPETSEKLLFVNAFTTHFTNYHTPSRVRFGQDAN
                     PGGPDLLRYLISQAYIPEYQVRWRWKPNSVAIWDNRSTQHYAVMDYAPCHRKMERAGI
                     IGDKPY"
     gene            complement(1245..2225)
                     /locus_tag="VHV99_27005"
     CDS             complement(1245..2225)
                     /locus_tag="VHV99_27005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEX3639068.1"
                     /translation="MAKAVRFHEVGGPEVLRYEDVEVGDPGVGQVRLRHEAVGLNFAD
                     TYFRSGLYPVPLPSGMGVEAAGVVEAVGDGVTNVAVGDRVTYTGFVNTLGAYSTERLC
                     PAAPLIRLPEGITCETAAAMTMRGLTSSYLMRRIYDFKAGDTILLHAAAGGVGMIVSQ
                     WARLLGLTVIGTVSSEAKAEVAHAYGCEHTINYSHEDVAARVRELTGGVGVNVVFDSV
                     GKDTFDATLNSVKRRGLIVCVGTASGPIPAFNPQILAMKGSPYLTRPALADYIADPAE
                     KADLAAEIFGHVAAGRIKIEINQRYALEDAARAHRDVESRRTTGSSVFVI"
     gene            complement(2936..3304)
                     /locus_tag="VHV99_27010"
     CDS             complement(2936..3304)
                     /locus_tag="VHV99_27010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX3639069.1"
                     /translation="MKRFQAFKLAVGVAIIAASMSAWSQSSETASAPAMAAPGSAAPA
                     TGRKANRALRRQVYNAIVKQKDISAGNISVVAKDGAVTLNGTVTDASQVGRVAEIARG
                     VPGVTSVTNKLTVQKPLGGM"
     gene            complement(3331..4476)
                     /locus_tag="VHV99_27015"
     CDS             complement(3331..4476)
                     /locus_tag="VHV99_27015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018434518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX3639070.1"
                     /translation="MKLKLSSTAALLMFATAAHAQSSVTLYGVLDSGLLYQSTSAASF
                     QPTARNQGHVYQLKDGGIYASFWGIKGSEDIGGGYKINFKLQGSFNSATGKSGLSDTP
                     GTVAMFNQYATVGASGPFGTFDAGRQIIPMIYAMAETDVRGAQYFGSILTAWLGVNQA
                     AGWPGTSTNAPIGALYDSNALVYSSPKFYGASLALEYAPGGVAGQFQGGTRESAVLKY
                     SNYGLNLSAVYYNGHDTNPFPLTYPTTPAVPATGQANNRFYYFGAMYTIAGFSISGSY
                     GIGKNPAKSNTADFEMASGGLGYQFNSRFKITSGYYYLKDRNHSANHSSEVAVGAEYS
                     LSQRTRAYAQVGYVDNKGTMNQTIIYGSPVAPGVSTTAAMIGIRHTF"
     gene            complement(4555..6177)
                     /locus_tag="VHV99_27020"
     CDS             complement(4555..6177)
                     /locus_tag="VHV99_27020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(methylthio)propionyl-CoA ligase"
                     /protein_id="HEX3639071.1"
                     /translation="MQGLMMQQPLLVASLLVHADRHHGEQEIVSRRVEGDIHRYRYRD
                     LAQRARRMANGLAALGIKSGERVGTLAWNGYRHMELYFAVSGSGSVLHTLNPRLHVDQ
                     LAYIIEHAEDRVVFFDLTFLPLIESVAPRVKSPKVFVAMTDRAHMPQAHGLSSTLMCY
                     EDLIERHSDAFDWPLLDENSASSLCYTSGTTGNPKGVLYSHRSTLLHTYAAALPDSLN
                     CSARDVILPVVPMFHVNAWGLPYIACMVGAKLVFPGPALDGKSLYELIEAEQVTLSAG
                     VPTVWQGLLSHVAERGGAFSSMRRTVIGGAPCPTAMTAQFQERYDVDVLHAWGMTELS
                     PVGTVCSFKAHQRALCGKERYAVQAKQGRSVFGIDMKIVGLDGEELPWDGKAAGDLLV
                     RGPWVARQYFGGGDASPLRDGWFPTGDIAKIDADGFMQITDRSKDVIKSGGEWISSTD
                     IENAACLHPEVTVAVCIAARHPKWDERPLLLVVRKSTSMLTEAQLLAFLDGRIAKWWK
                     PDAVIFVEEIPLGATGKVLKHRLRDQYGSYYL"
     gene            complement(6311..7627)
                     /locus_tag="VHV99_27025"
     CDS             complement(6311..7627)
                     /locus_tag="VHV99_27025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3639072.1"
                     /translation="MLMQHAEPTTGDVPATQTDVRPYAWVVFALTVGLLLSDYMSRQV
                     LNAVFPLLKAAWDLSDTRLGSLSSVVALMVGVLTFPLSVLADRWGRVKSVVLMAAMWS
                     LATLGCAIATNYGEMLLARAFVGIGEAAYGSVGIAVLLSIFPPRLRATLTGTFMAGGA
                     FGSVLGMALGGAVAARMGWRPAFGAMAAMGIVLVIVYRFVVTEKRLAPLQPAGLGNQR
                     LGVRMSFRALMKGLFSTKSVVCAYVGSGIHLLVPAAVWAWMPSFLNRYYGLQTGKAAL
                     GAAGFVLVTGLGMVVCGNLADRLGKQGRDRKWGAAIAICLTCFTLLAIGIRMPAGPLQ
                     LALIGAGMFFSAGASGASGAMVANLTQPSIHSSAFATLTLANNLLGLAPAAILTGIVA
                     DRIGLLGALQLVPFASLVAALAFFIGKRNYSGDLERLDALREQAER"
     gene            8069..9070
                     /locus_tag="VHV99_27030"
     CDS             8069..9070
                     /locus_tag="VHV99_27030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX3639073.1"
                     /translation="METMLRSVGMSGYFDVTRRLGLNPVELVQQLGIDPAALANPEDR
                     VPAAACCRLLELTADKASCPTFALQMAETRQKFGTGVVNILLAHKRTLREVLRAAAEF
                     RHLLNEALAVYVEDAGETVTIREELVVDPGTPTRQAIELAVGILARHSSALLGSHWKP
                     RAVHFTHREPADPTFHRRFFGCQLEFGSDFNGFVCAAAELDYPNPAADLELVRYAESL
                     ATPLNAAATDSIELEVRKAIYLLLPVEQGTVDHVARHLRLSVRTMQRQLGSADTSFSA
                     LVDEVRRELAIRYMSNPRYPVGRVAALLGYNQQGSFTNWFASRFGMTPRDWRNGQAK"
     gene            complement(9252..10310)
                     /locus_tag="VHV99_27035"
     CDS             complement(9252..10310)
                     /locus_tag="VHV99_27035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016347288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEX3639074.1"
                     /translation="MNFLDGSLYTENQQPLIITAAPYAPSWVPSDFPEDIPVTIDEQV
                     QKAVDCYNAGATVLHLHVRELDGKGSKRLSKFNELIAGVRNAVPEMIIQVGGSISFAP
                     ESEGAAAKWLSDDTRHMLADLDPKPDQVTVTINTSQMNVVEHWEDADVAGTSMEDPVV
                     YNAYKEMTVPAQPGWAEEHIRRLNAAGIQSAFQCYNINSFESVERLIRRGVYKGPLVM
                     NWVAIGGGMDAPNIYNLANFVRATPDGAVLTVESSMRNVLPVNMMGIAMGLHVRCGIE
                     DNLWNQSRTKKMSTVEQIEQLVRITREFGRKVATAQEAREISKIGVFYDTVEETLLAN
                     GLAPNRNGGTQGFLRKVV"
     gene            10638..10770
                     /locus_tag="VHV99_27040"
                     /pseudo
     CDS             10638..10770
                     /locus_tag="VHV99_27040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558189.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT         2092 a         3489 c         3325 g         2134 t
ORIGIN      
        1 gcaacggcag ccacccgtgc tcttcgagca cgtccaacgc cggataaccg gcgggcatag
       61 gaacctcaag gtccgccggc gtggccagca gcgcgccctg gatcgggcgg ttatggcgct
      121 gcgcccagtg cacggtaatc atgacgccgg cgctgtgagc gaccagtacg atcgggccat
      181 cgatcttcgc gatggcctgg tccagcgccg ccacgcgcgc cgcgcagctg agcttgtcgt
      241 gctcgagcgg cgggaccgag gccgttttgg aaagacgagc ctgcagcaat gtctgccagt
      301 gctcggccac atggtcgcgc agaccgggca cgataagtac ggtaggtgcg gtattgagcg
      361 tcacgacgct aagtccttca ggttagtacg gcttgtcgcc aatgattccg gctcgctcca
      421 tcttgcgatg gcacggcgcg taatccatca ctgcgtagtg ctgcgtgctg cggttgtccc
      481 atatcgcgac gctgttcggc ttccagcgcc agcgcacctg gtactccggg atgtacgcct
      541 ggctgatgag ataacgcagc aggtcgggcc cgcccggatt ggcgtcctgg ccgaagcgca
      601 cgcgcgacgg cgtgtggtag ttcgtgaagt gcgtcgtgaa cgcattgacg aacagcagtt
      661 tctcgctcgt ctcggggtgc gtgcgcacga ccggatgttc cgcatcggga tacagcgctt
      721 tcaaggcgtg ccgcttctcg atcggcatca ccgcaccgaa gctcgcctcg atactgtggc
      781 gtgcgcgcag gtcggcgatc tgttccttca cgtgcgcggg caggttctca tacgcgagca
      841 ccatgttcgc ccacatcgta tcgccgccga cgggcgggca ttccacacac cgaagcacag
      901 caccgaactg ggggatctcg cgccaggtgg cgtcagaatg ccatgcgttt tcgtagcggt
      961 cgttgggctg gtccggcgac ttgtaaattc gcacgagtcc cggatgctca ggatcgctgc
     1021 ccgcgaccgg atggtcttcc agctcaccga agcggcgcgc aaacgcgaca tgctcggcac
     1081 ggctaatgtc ctgatcgcgc aggaacagca cccggtgcct gagcaggttc gcccggatct
     1141 cggcaaagag gccgtcgtcg tgaatcgcgt cggcgagcct gatgcccacc aattccgcgc
     1201 caatggcgtt attgagttgt tcgacacgca tgtcgctctc ctgcttaaat gacgaagact
     1261 gatgagccgg ttgtcctgcg cgattcgaca tcacggtgtg ctcgcgcggc atcttcgagc
     1321 gcatagcgct gattgatctc gatcttgatg cggccagccg caacgtggcc gaagatttcc
     1381 gcggccaggt ccgctttttc ggcggggtcg gcgatatagt cggccagcgc gggacgggtc
     1441 agatagggcg atcctttcat tgccaggatc tgcggattga atgccgggat cggccccgat
     1501 gccgtgccga cgcaaacaat caggccgcgg cgcttgactg aattcagcgt ggcgtcgaag
     1561 gtgtccttgc cgacgctgtc gaaaaccacg ttgaccccga cgccgccggt caactcgcgc
     1621 acacgcgcgg cgacgtcttc atggctgtag ttgatcgtgt gttcgcagcc gtaggcgtgg
     1681 gcgacttccg cttttgcctc gctcgacaca gtgccgatca ccgtgagccc cagcagcctc
     1741 gcccactgcg agacgatcat cccgacgccg cccgcggcag catggagcag gatggtgtcg
     1801 cccgctttga agtcgtagat gcgccgcatc agatacgacg aggtcaagcc acgcatggtc
     1861 atggctgccg cggtctcgca ggtaatgcct tcgggcaacc tgatcagcgg cgcggcggga
     1921 caaaggcgct ctgtgctgta ggctcccaac gtattgacaa aacccgtgta ggtgacacga
     1981 tcaccgaccg cgacgttggt cacaccgtcg cccaccgctt cgactacgcc ggctgcctcg
     2041 acacccatgc cggaaggcag cggaaccgga tataaaccgg agcggaaata ggtgtccgcg
     2101 aaattgaggc caactgcctc atggcgcagg cgaacctggc ccacgcccgg atcgccgact
     2161 tcgacgtctt cgtaacgcaa gacttcaggg ccaccaactt catgaaagcg gactgctttc
     2221 gccatgattc gttctccagg ttgtcgcttg atttacagaa ggggaatgct gctgaaggac
     2281 cgtttcgtct ccggcgcacg tgccaatcaa cccaagttat ccaggtaatt tcgtttcctt
     2341 cgcttgagtc gcgtcgcgcg cctttttcct gtaccgacaa aagatccgat atttgtgtcg
     2401 tctctcgaag caggtcgggg cgcttccatc ttcaaccgcc gtcggcacga aatgtcaatc
     2461 gccgtgctga acgagtgttg ccgccgcatc aggtatgttg acgcgacgga agaggatcag
     2521 tcgagtgaag atacggtaat ccggcgatgc ggatttcgtt tttggtgaca ttgttttatg
     2581 atttgacgac agtgcgcgtt aacaccaacc cgccactaac ccgatgccgc tttccagctc
     2641 ggcactcgcc acgccccggc ctgggcgggt gtaaacccga acgcgctgcc tgtccacatc
     2701 agatcgtcaa gaagctgcga ggctgcactg cttcgaaaac acgcaggacg gatctcttca
     2761 atcagtaaca gttggtatag aaagagatga acgaaattgg cggctacgat agtgaaggcg
     2821 caaagccagg ctttcgcctc aacgaacgcc tgcccggata tcaccaccgg ccgtaaacgt
     2881 ggacggggcc gatacagtca tcggccccgc tgcgctgcgt cacgatttcc gcagcttaca
     2941 tgccgcccaa tggcttctgg acggtcaact tgttagtaac cgaagtaaca ccgggaacac
     3001 ccctcgcgat ttccgcgacc ctgcccacct gagaggcgtc cgtgactgtg ccgttcagtg
     3061 tcaccgcgcc gtcttttgcg acgacgctaa tatttccggc actgatgtcc ttttgcttca
     3121 cgatagcgtt atatacctgc ctgcgcagtg cacgatttgc ttttcttcct gtggcgggag
     3181 ccgctgaacc cggcgccgcc atcgcaggcg cgctggccgt ttcgctggac tgcgaccatg
     3241 cgctcatcga cgcagcaatg attgcgacgc caacagccag tttgaatgcc tgaaatcttt
     3301 tcattgaata ctccggtgag gatggcgcaa tcagaaggta tggcggatgc cgatcattgc
     3361 cgccgtggtc gagacgcccg gcgcgaccgg agacccataa atgatcgtct ggttcattgt
     3421 ccccttgttg tcgacatagc cgacctgtgc gtacgccctg gtccgctggg agaggctgta
     3481 ttcggcaccc acggccactt cgctcgagtg attggcggaa tggttccggt ccttcagata
     3541 gtagtagccg gacgttatct tgaagcgcga attgaattga tagccgaggc cgcccgaagc
     3601 catttcgaag tctgccgtgt tgcttttcgc cggattctta cctatgccgt acgagccgga
     3661 tatcgagaat ccggcgatcg tgtacatcgc accgaaatag tagaagcgat tgttggcctg
     3721 gcctgtcgcg ggcacggcgg gcgtggtggg ataggtcaac ggaaaaggat tggtgtcgtg
     3781 tccgttgtaa tagacagcgg agagattcag accatagttc gagtacttaa gtaccgccga
     3841 ttcgcgcgta ccaccctgga actggccggc aacaccgccg ggcgcgtatt cgagcgcgag
     3901 cgaagcgcca tagaattttg gcgaactgta gaccagcgca ttgctgtcat acagggcgcc
     3961 gatcggcgcg ttcgtgctgg tgccgggcca cccggccgcc tgattgacac ccagccacgc
     4021 ggtcaggatg ctgccgaaat attgtgcgcc gcgcacatcg gtttccgcca tcgcgtagat
     4081 catcgggatg atttgccggc ccgcgtcgaa tgtaccgaac ggacccgatg ctccgacggt
     4141 cgcgtactgg ttgaacatcg cgactgtgcc cggcgtgtcg gacagccccg atttgccggt
     4201 cgccgagttg aacgaaccct gcagcttgaa attaatcttg tagccgccgc cgatgtcttc
     4261 gctgcccttt atgccccaga agctggcgta gatgccgccg tccttgagct gatagacgtg
     4321 cccctggttg cgggcggtgg gctggaacga ggccgccgag gtgctttgat acagcagtcc
     4381 actatccagc actccgtata gcgtgaccga cgattgcgca tgggcagcag tcgcgaacat
     4441 cagcagcgcc gcggtgctgc tcaatttcaa cttcagtttc attctgtctc ctgtggttcg
     4501 gtttatggtt tttgcgcgaa tcaatttaag gtgctgctac gtaagccggc gaggtcacag
     4561 gtagtagctc ccgtactggt cccgcaaccg gtgcttgagt accttgccgg tcgcgccaag
     4621 cggaatctct tcgacgaaaa tcacggcgtc cggcttccac cacttcgcaa tcctgccgtc
     4681 caggaaggcg agtagttgcg cctcggtcag catgctggtc gactttctaa cgaccagcag
     4741 caacggccgt tcgtcccact tcggatgacg cgcggcgata cagaccgcca ccgtcacttc
     4801 cgggtgcagg cacgcggcat tttcgatgtc ggtcgaactg atccattcgc cgccggactt
     4861 gatgacgtcc ttgctgcgat ccgtgatctg catgaagccg tcggcatcga tcttcgcgat
     4921 gtcgccggtc ggaaaccagc cgtcgcgcag tggcgacgcg tcgccgccgc cgaagtattg
     4981 acgcgcgacc cacggaccgc gcaccagcag gtccccggcc gccttgccgt cccacggaag
     5041 ctcctcgcca tcgagaccga cgatcttcat gtcgatgccg aatacggagc ggccctgctt
     5101 cgcctgaacc gcgtagcgct ccttgccgca cagagcccgc tgatgcgcct tgaagctgca
     5161 gaccgtgccg accgggctca attccgtcat gccccatgcg tgcagcacgt cgacgtcata
     5221 gcgctcctga aactgcgcag tcatcgccgt cggacacggc gcaccgccga tcaccgtgcg
     5281 acgcatcgat gagaaagccc cgccccgttc ggccacatgc gagagcaacc cctgccagac
     5341 cgtcggcacg ccggccgaca gcgtgacctg ttccgcctcg atcagttcgt agagcgattt
     5401 gccgtcgagc gcaggccccg gaaacaccag cttcgcgccg accatgcacg cgatgtacgg
     5461 cagcccccac gcattcacat ggaacatcgg aaccacgggc aggatcacat cgcgcgcgga
     5521 acagttcagc gagtccggca gcgctgcggc gtacgtgtgc agcagcgtcg aacgatggct
     5581 gtacagcacg ccctttgggt tgccggtcgt gccggaggtg tagcagagcg acgaggcact
     5641 gttctcgtcc agcagcggcc aatcgaacgc gtcgctgtgg cgctcgatca ggtcctcgta
     5701 gcacatcagc gtgctggaaa ggccatgcgc ttgcggcatg tgcgcgcgat ccgtcatcgc
     5761 gacgaacacc ttcgggcttt tcacccgagg cgcgacgctc tcgatcaacg gcagaaaagt
     5821 cagatcgaag aacacgacgc gatcttccgc gtgctcgatg atgtacgcca gctggtcgac
     5881 gtgcagccgc gggttgagcg tatgcaggac ggagcccgat cccgacaccg cgaaatacaa
     5941 ctccatatgc cggtagccat tccacgccag cgtccctacc cgctcgccgg acttgatgcc
     6001 gagtgccgca agaccgttcg ccatgcgacg cgcgcgttgc gcgagatccc gatatcgata
     6061 ccggtgaatg tccccttcaa cacgtcgcga cacgatttcc tgttcaccgt gatgacgatc
     6121 ggcgtgtacg agcagcgacg ctaccagtag cggttgctgc atcatcagac cttgcatcct
     6181 tcttctcctg agtgtttatg tgttcccgcg ccggggtcga gtcagtagcg cctcgcgtcg
     6241 ggccttccgg cgcgtcctct gctgggaccg cacggtgcat catccgtcgc tgcgtgccag
     6301 cgagaagtcc ttagcgctcg gcctgctcgc ggagggcatc cagccgctcc agatcgccgg
     6361 agtagttgcg cttgccgatg aaaaaagcga gcgcggcaac gagcgacgcg aacggaacga
     6421 gttgcaacgc gccgagcagc ccgatccggt cggcgacgat gcccgtcaga atggccgcgg
     6481 gcgccagacc cagcaggttg tttgccaacg tcagggtggc aaacgcggac gagtgaatcg
     6541 acggctgggt gagattcgcc accattgcgc ccgaggcacc gctcgcgccg gcgctgaaga
     6601 acatgccggc gccgatcaac gcgagttgca gcggacccgc cggcatgcga ataccaatcg
     6661 cgagcagcgt gaaacacgtc aggcagatgg cgatcgcggc gccccacttc ctgtcccgcc
     6721 cctgcttgcc gagccggtcc gccagatttc cacacaccac catgccaagg cccgtcacca
     6781 gcacgaaccc tgccgcgccc agtgcagcct tgccggtctg caagccgtag tagcggttca
     6841 ggaagctcgg catccaggcc cacacagcag caggaacgag cagatggatg ccgctgccga
     6901 cgtaggcgca gacaaccgac ttcgtcgaaa agagtccctt catcagcgcg cggaaactca
     6961 tgcgcacgcc aagccgctga ttgcccaggc ccgcaggctg gagcggcgca agacgctttt
     7021 ctgtgacgac gaaccggtag acgatgacca gcacgattcc catggccgcc atcgcgccga
     7081 acgccggacg ccagcccatg cgagccgcga ccgcaccacc gagcgccatc cccagcacag
     7141 agccgaacgc tccgcccgcc atgaacgtgc cggtgagcgt ggcgcgcagc ctcggcggaa
     7201 agatactcag gagaactgcg atccctacgc tgccgtaagc ggcttcgccg atacccacga
     7261 aggcacgcgc gagcagcatt tcgccgtagt tcgtcgcaat cgcgcagccc aacgtcgcga
     7321 gactccacat tgccgccatc agcacgacgc ttttcacgcg gccccagcga tcggccagca
     7381 ccgacagcgg gaacgtgagc acgccgacca tgagcgcgac cacactgctc agggaaccca
     7441 gccgtgtatc agagaggtcc catgcggcct tgagcagagg aaacacggca ttcagaacct
     7501 gccgcgacat gtagtcggag agcagcaagc caaccgtcaa ggcgaagacc acccaggcat
     7561 acgggcgcac gtcggtttgt gttgctggaa cgtcgcccgt ggtgggctcg gcgtgttgca
     7621 tgagcatcat tcgtctcctt cgcgtttatc acgcgtttat cacgcgttca tgtcgcgtct
     7681 gtctagcgtc tgtctagcgt ttgcctggcg tttgcctggc gtttgcctcg gctgcagccg
     7741 tgcctgttcg tagcgaaaga cgaccggctc tggatacctc gccgtctccg ctgcacgaca
     7801 ttaggcatcg gcttcctgac ccgcatccct gaagcagtcc tgccggtcgc aatcccgcgc
     7861 ccggtgacgc gtgaagcgcc tgaaaacttt cgggatcgaa accaacatcg tgctcgcaca
     7921 atacgaccat cgcgcattac acttttatga ttgggcgcca tccttttagt gctagatgcc
     7981 actgcgtgta aacaccggtc atcgttcccg caggcatctg atgccggaaa acgaggcgcc
     8041 gctcatccga gccccatgga ggaaagcgat ggaaacgatg ttgcgctcag tcggcatgag
     8101 cggctatttc gatgtgacgc ggcggctcgg actgaatccc gtcgagctcg tgcagcaact
     8161 ggggatcgat cccgcggcgc tggcaaatcc ggaggaccgc gttccggccg ccgcatgctg
     8221 ccgcttactc gaactcacgg ccgacaaggc gtcgtgtccc actttcgcgc tgcagatggc
     8281 tgaaaccagg cagaaattcg gcaccggcgt cgtcaatatc ctgctcgcgc acaaacgcac
     8341 gttgcgtgaa gtgttgcggg ccgccgccga attccgccat ctgctcaacg aagccctggc
     8401 cgtgtacgtc gaggacgctg gggaaacggt caccatcagg gaggaactcg tcgtcgatcc
     8461 gggtacgccg accaggcagg cgatcgaact ggcggtgggc atccttgcgc gccactccag
     8521 cgcgctgctc ggcagccact ggaaaccgcg cgccgtgcat ttcacgcacc gggagcccgc
     8581 tgatccgacg tttcatcgac gcttcttcgg ctgtcaactg gaatttggca gcgatttcaa
     8641 cggcttcgtg tgtgccgccg ccgagctcga ttacccgaat cctgccgctg acctcgaact
     8701 ggttcgatat gccgagagcc tcgctacgcc actcaacgca gcggccacag attcgatcga
     8761 actggaagtg cgcaaggcaa tctacctgct gctgcccgtc gagcagggca cagtcgatca
     8821 cgtggctcgc catttgcgcc tgagcgtgcg caccatgcag cggcagctcg ggtcggccga
     8881 taccagcttc tcggcgctgg tcgatgaagt tcgccgcgag ttggccatac gctatatgag
     8941 caaccctcgc tatccggtcg gaagggttgc ggcactgctc gggtacaacc agcagggttc
     9001 tttcacgaac tggttcgcgt cacggttcgg tatgacgccg cgtgactggc gtaacggcca
     9061 ggcaaaatga aatgcacgga cacttgctgc cgtgcgtcag cgtgcctgat cgaaataggc
     9121 aggacttcag tgccgaccgt gaacggtctt cgcgtgttgc cgaccatcag gcagtcgcac
     9181 gggcccccgg catggccggc tctgaagcgc cgctttcatg tgcgagcaac ctgccccgtc
     9241 ggctcacgcc ttcataccac cttgcgcagg aagccctgag tgccaccgtt gcggttgggg
     9301 gcgaggccgt tggccagcaa ggtctcttcc actgtgtcgt aaaacacgcc aatcttgctg
     9361 atctctcgcg cctcctgggc ggtggccacc ttgcggccaa actcgcgtgt gatgcgtacc
     9421 agttgctcga tctgctcgac cgtgctcatc ttcttcgtgc gtgactggtt ccagaggttg
     9481 tcctcgatgc cacagcgcac atgcagaccc atcgcaatgc ccatcatgtt gaccggcagc
     9541 acgttgcgca tcgaactctc cactgtcagc accgcgccgt ccggcgtggc gcgcacgaag
     9601 ttggccaggt tgtagatgtt cggcgcatcc attcccccac cgatggccac ccagttcatc
     9661 accagcgggc ccttgtagac gccgcgccgg atcaggcgct caaccgactc gaagctgttg
     9721 atgttgtagc actggaacgc gctctgaatg ccggctgcgt tcaggcgacg gatgtgctcc
     9781 tcggcccagc cgggctgcgc cggcactgtc atctccttgt aggcgttgta gaccacgggg
     9841 tcctccatgg aggttcccgc gacgtcggca tcctcccagt gttcgacgac gttcatctgc
     9901 gaagtattga tcgtcaccgt cacctggtcg ggcttggggt cgagatcggc cagcatgtga
     9961 cgcgtgtcgt cgctcaacca tttggccgcg gcaccctcgg actcgggtgc gaagctgatc
    10021 gagccgccca cctggataat catctcgggc acggcattgc gcacgccggc gatcagttcg
    10081 ttgaacttcg acaggcgctt gctgcccttg ccgtcgagct cgcgcacgtg cagatgcagc
    10141 accgtagcgc ctgcgttgta gcaatccacc gccttctgga cctgctcgtc tatcgtgacg
    10201 gggatgtctt ccgggaaatc ggagggcacc cacgacggag cgtaaggcgc ggccgtgata
    10261 atcaaaggtt gctggttttc ggtgtaaagg gaaccgtcga gaaagttcat ggcgtggtct
    10321 ccaggcgcgg gttgagggga aagaaagaga tcggggagcg ctcatgcagc gctgtcgtca
    10381 accaggccac tggccaacag aaaccctctc ccccttcccc gtaggtaacg gacggatcag
    10441 ctccgcatca ggctggtgta gccgggacgg gagattgaac agtgaggcga agatacggca
    10501 atccggcaag gtactttacg gattggatgc catttttttg tgatttaacg ccactacgtg
    10561 ttaacaccca ttcgtcgcgg acacgcttga tgggcaaaca gtggtttgcc agggtaggcg
    10621 agatactgca aggttgcggt ctgaagatcg gcaccggcac ccatcgtgga tgcgaccatt
    10681 atcggcgcgc cgggttcgac gaagaacaag gcgcgagacc ccgagatgca tcagacacga
    10741 aagggaaagc agtggtactt cgggagagaa ctcatatcgg ggtcgacagc cagacggcgc
    10801 gcattcctac gccagaaggt cgtccgcatc gtccgtgcgg cgcaccgctt gccccgcgcc
    10861 cgtcattacc ggccaggcga tctggcgtat ctggggcagg ccgggttttc actttcacag
    10921 attgagtcgt gtcaccttgg cgccgttgag cgaagcgtcc gccatcagac cggtattggt
    10981 cagcacgagg gccacgacct gcgaggaggc tgatgtcgtg tcgattgtgc cgttcgcacc
//
LOCUS       DATASH010001018         2386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3140624,
            whole genome shotgun sequence.
ACCESSION   DATASH010001018 DATASH010000000
VERSION     DATASH010001018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3140624"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            245..1300
                     /locus_tag="VHV99_27045"
     CDS             245..1300
                     /locus_tag="VHV99_27045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013338213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3639075.1"
                     /translation="MGRAFKKVTFSPKTIGALAAAATAAAAGALWVQYRAHKAERDNP
                     PIGRVVEVDGIPLHYVDKGKGPSVVLLHGNAVLLQDFVASGLVDRLAERHRVIAFDRP
                     GFGYSGRPRDRLWTAQAQAALIQQALSLLGVEQPVVLGHSWGTLVALGMAVGDAADVR
                     GLVLVSGYYYPTARVDVALAAPAALPVLGDVLRYTVSPLTARLLLKRSVEVMFAPAPM
                     PAGFFDVIPREMLLRPLQIRAEAEDAAFMIPAAAQLSARYSDLKMPVSIFAGAKDKIV
                     DPESNSARLHHAVAHSTLAVASEAGHMVHYALTGEIVEAIDLMTARQNAQSNAGDAII
                     SDTSSETEMTEDAPATT"
     gene            1385..2311
                     /locus_tag="VHV99_27050"
     CDS             1385..2311
                     /locus_tag="VHV99_27050"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX3639076.1"
                     /translation="MNGSIDCVIVGGGPAGLTAALYLARYRRTTRVFDAGESRARLIP
                     VARNVPGFPSGISGEAWLVNLREQLTPYDVAPEHAQVDAIEAVSDGFRVDYRPMRGGD
                     AETARPVHARRVLLAAGIRDGLPLVPNADELTRSGLFRLCPICDGYETEGKRIAMLGP
                     AKCALSHAIFMRTFSRDVSVVASDLDALSGAEVDSAAAGGVSLIALSRLEAPRGANQA
                     VRIIGLSGAIHRFDSVYPVMGCHPRTDVLGLEVERDEDGMVRTDKHQRTSIQHVYAAG
                     DIVNTLNQISVAAGEAAIAATAIHKSLPHNPR"
BASE COUNT          437 a          710 c          751 g          488 t
ORIGIN      
        1 cgcgtatgtg ttgcatgccg cctgctgtag ttcgatatca ggtcggcgat cggtccctac
       61 ggacggcacc gctaccagcg cgcggtcgcg gcatgctcgt tgcgggatgg aagaaagttg
      121 ggaatcctac gttggccgcg cgaatcagag cggaccaaat catcgagcat ctacgcgctg
      181 cggccgggcg gccgattctc caccaggaga tcgccttccg cctgtggcca ttacaggaga
      241 agtcatgggt cgcgcattca agaaagtcac tttttccccg aaaactatcg gagcgctcgc
      301 agcagcggcc actgccgcag ctgccggggc gctgtgggtt caatatcgtg ctcataaagc
      361 cgagcgcgat aatccgccaa tcgggcgcgt ggtcgaggtc gacggaatac cgcttcatta
      421 tgtcgataag ggtaaagggc cttccgtggt gctcctgcat ggcaatgcgg tgctgcttca
      481 ggacttcgtc gccagcgggc tcgtcgatcg gttagccgag cggcacaggg tgatcgcgtt
      541 tgatcggccg ggcttcggtt acagcgggcg accacgcgac cgtctctgga ccgcgcaagc
      601 gcaggccgcc ctcattcaac aggcgctctc gctgctgggc gtagagcaac ctgtcgtgct
      661 cggacattcg tggggcacgc tggtcgcact tggcatggcg gtcggtgacg ccgccgacgt
      721 gcgcgggctc gtgctggtct ccggttacta ctatccgact gcgcgcgttg acgtcgcgct
      781 cgctgcgccg gccgcacttc ccgtgctcgg ggatgtcttg cgttacaccg tttcgccgct
      841 gaccgctcgc ctgctgctga agcgcagcgt ggaagtgatg ttcgctccgg cacccatgcc
      901 agccggcttc ttcgatgtca ttcctcgtga aatgctgctg cgtcccctgc aaatcagggc
      961 cgaggccgag gacgccgcgt tcatgatccc ggccgcggcg cagttaagtg ctcgttattc
     1021 cgacctgaaa atgccggtca gtatttttgc cggcgcgaag gacaagattg ttgaccctga
     1081 atcgaattca gcccggctgc accacgcggt ggcccatagc acactcgccg ttgcgtccga
     1141 ggcggggcat atggtgcatt acgcgctaac cggtgaaatc gtagaggcaa ttgatttgat
     1201 gactgcccgc cagaatgcgc agtccaatgc tggcgatgcc atcatcagtg acacgtcatc
     1261 cgaaactgaa atgacagagg acgcgccagc aacgacatga cgcgcagtcg acgctcgctg
     1321 tctgtagcga gcgtcgactg aagagggttg tcatactcat tgtgcctaag atggatactt
     1381 tccgatgaat ggttcgatcg actgtgtaat cgtcggcgga gggccggccg gcttgaccgc
     1441 ggcactctat cttgcgcgct atcgccgaac gacgagagtc tttgacgccg gcgaaagtcg
     1501 cgcgcggctt atccctgttg cgcgcaatgt accgggcttt ccgtcgggca ttagcggtga
     1561 ggcctggttg gtgaacctgc gcgagcagct cacgccgtac gatgtggcgc ccgaacatgc
     1621 ccaggtggat gccatcgagg ctgtcagcga tggtttccga gtcgactatc gtccaatgcg
     1681 aggcggtgac gcggaaacag cgcgcccggt gcacgcgagg cgtgttttgc tcgccgcggg
     1741 catacgtgac ggactgccac tggtgcccaa cgccgacgag ctgacacgtt ccggcctgtt
     1801 ccgcctttgt ccgatatgcg acggttacga gactgaaggc aaacgtattg cgatgttagg
     1861 cccggcgaaa tgtgcgctat cgcatgccat ctttatgcgg acattttcac gcgacgtgtc
     1921 cgtggtcgcg tccgacctcg acgcgctatc gggcgcagaa gtggattcgg ctgccgccgg
     1981 tggcgtcagt ttgatagcgt tatcgagact cgaagccccg cggggcgcta accaggcggt
     2041 acggatcatc ggcctgagcg gagctattca ccgtttcgac tcggtctatc ccgtcatggg
     2101 ttgccatcct cggactgacg tactgggact cgaagtggag cgcgacgagg acggaatggt
     2161 gagaactgac aagcatcagc gtaccagcat tcaacacgtt tacgcggccg gcgacatcgt
     2221 caataccctc aatcagatca gcgttgccgc aggcgaagcg gccattgccg cgacggcgat
     2281 tcataagagc cttccacaca acccacgtta gagcgcttgc gaattgcgct gaccaaggcg
     2341 catcagcgct gcggctccgt gtccgatatt tccgctgcga tggagc
//
LOCUS       DATASH010001019         1667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5121418,
            whole genome shotgun sequence.
ACCESSION   DATASH010001019 DATASH010000000
VERSION     DATASH010001019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5121418"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..1482)
                     /locus_tag="VHV99_27055"
                     /pseudo
     CDS             complement(114..1482)
                     /locus_tag="VHV99_27055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011578756.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
BASE COUNT          305 a          498 c          538 g          326 t
ORIGIN      
        1 gacgcgattg atcggtccgt cgtggtggat cggtgcaagg gaacgcgtgc tgcaacgcat
       61 tggggttgat cagggccatg tcggcggctc atcgggcact caagccctgg ccttcacggg
      121 ccgcacgaca tgaggcagaa cgcgacggtg cggaacaatg ctgcgtccgg gcaactcgtc
      181 gggagctgga tccggatgcg cgcgatgtgt tcaatcacgc gcgcagcgat tttgatcaaa
      241 cactcgcgga tggtggcgaa ctcagctttg gcgagcgggc ttttcggcgg gatcgcggcg
      301 cgcacggcat gcatgagcca gaacgcggcg gtgtgaagca ccaaccgcac ctgattggcg
      361 atcgcactgt gacacgacaa tgcgatccga ggctaactgc gccttgtgca acttgatgag
      421 attttccatc tgcccgcgct ggcaatagat gttctcgtag aggtgctgtg ccgagccctt
      481 gagcgaggtg acgacatagc ggatgtcgac ctcctggcgt aggctggttt cgccggggat
      541 cggttgcaac gagcattcca gccgtgccac caccttgcgc ggccggctcc agcttccagc
      601 ctgatacatg acgctcgcga aggtgcgcag cttcggcttg cgggaaagcg catgatggaa
      661 gcgcagatag gtcgcaatgt cggcgaccag ggcatcgagc gcggcattgc cggcgagccc
      721 aaaaatgtag tcggcatcgt tgttttccgc ccactccatg gcctcgacgc ggccgtagtg
      781 gctgtcgccg cgccacacga tgcgggtctt gtgccaatgc cggcgcaggc gcttggtcac
      841 gtgcttgatg acagtcgcga cctcggtgcc tttgggagta cgcgccgggc gcaggatcgc
      901 cgccaccggc gtgccgctcg gcacgtgata gatgtgcatc gttgcaaaac cgcgctcgtc
      961 gtagtgggcg ttccacaacg ccaattgctg accgccatgc gcggcacaga acgtgtcgtc
     1021 gatgtcgaga acctccagct tgccaggttt gacggtcgta ccaaactgat cgaccagcgc
     1081 tgccgtcaac cgcgccgctt gggtcttgct cggcgtattc tccagccggc tgatggtcga
     1141 ttgcgatgcc agcgcggcac tgctctgcgg acagcggcca acggcgagtt ttagcagcgg
     1201 atcgtgccgc agccggtcca agtcgatggc gtccttatgc ccgcacgcaa tcgcagcggt
     1261 gcgtgccatc accagttcga acatgtcatg ctggatgcgg tccggatcgc ggcgatccgg
     1321 catcgcttgc gccagccgcc ggcaaacgcc aagtctgcgc tcggctgcgc gcaacagcaa
     1381 aagaccgcca tcggaggact ggttgccacc ttcgaaatcg actgtgagtt tcttgcgctg
     1441 gaccgatggc agatcgaagg gaagcactat atcgtcggtc atgcggctgt ggcgctcggc
     1501 tggtacacat tgcaattcgt gtgagcaacg atttgctacg tgaatcgcgc gtttcttgcc
     1561 acagccgtct caatatatct ccccgtcacg aataatccgg gctagagaca gttcaccgtg
     1621 ttgggccatc cgtagcgcga ggtacgccac gcagggcttc gacaggg
//
LOCUS       DATASH010001020         3395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6109030,
            whole genome shotgun sequence.
ACCESSION   DATASH010001020 DATASH010000000
VERSION     DATASH010001020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6109030"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VHV99_27060"
     CDS             <1..275
                     /locus_tag="VHV99_27060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX3639077.1"
                     /translation="FEVPRDMSIVGFDDMEFASLLMPPITTLRQSARELGRAGVAILL
                     AMLAADHGEAAGAAAALAPGAALGAPVHRLPVRLIERASVAPPRKT"
     gene            461..1405
                     /locus_tag="VHV99_27065"
     CDS             461..1405
                     /locus_tag="VHV99_27065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX3639078.1"
                     /translation="MNAPHYLRRLSVTLFALSLIGSSGSAFAADKKIALVQINQEALF
                     FTQMDQGAQAAAKAAGVNLEIYNSNNDPNAQNNAIETYIQQKVDAILVVAIDVNGIKP
                     AISEAKKAGIPVVTIDAIVNGDNAVQVGVDNKKVGVDIGKYTANYINKNLGGKAQIGV
                     VGALNSFIQNVRLDGFKEGVATPPGAKIVSTVDGQNVQDQAQTAAENLISGNPNLQIV
                     YATGEPALIGVVAAVLSQGTTDHLKIVGWDLSAQAIKGIDAGFVTAVVQQDPEGEGKA
                     AVGAALKLIAKQPVEKNISVPVTIVTKANVDKYRAIFK"
     gene            1456..2244
                     /locus_tag="VHV99_27070"
     CDS             1456..2244
                     /locus_tag="VHV99_27070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3639079.1"
                     /translation="MNSVAITPRVRMENIIKSYGAVQSLRGVNLEVAPGEVLGLVGDN
                     GAGKSTVSKVLAGAVIPDGGRIEIDGNPVHFTDPGDARAHHIEMVYQDLSLCDTVDVA
                     GNLFFGREPLRRVLGIPMLDHAKMHHDAREMLDGLGIRIPNTHLDVENLSGGQRQAIA
                     ISRAVSFDPKVLIMDEPTAALAVAEVEAVLELVQTISQRGVSVILISHRLQDLFLVCD
                     RISVMYEGQNVADRKIGETNLEEVVDLIVGRKFKARSARALSED"
     gene            2247..3248
                     /locus_tag="VHV99_27075"
     CDS             2247..3248
                     /locus_tag="VHV99_27075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004954891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3639080.1"
                     /translation="MEIAMHKDQRAPRRSLQHLLGGNAGVASIAVFFVCCAVAFSFAT
                     DTFLTAGNLLNVLRQSAPLLIVATAMTLVITTAGIDLSIGSTLALVGALAAIALHAGM
                     SDSVVLIGGLLLGALIGAINGYFIAWAGMPAFIVTLGTLSVVRGLALLITQGYSVPIE
                     NDGWFVQLGRGHILGVPMPAIISAVILFAGWFTLRHMRFGRYVVGIGTNAEGVRRAGV
                     NVRIVTLKVYILSGAAAGLAGLITTARLGSGSSNQGVGFELAVIAAVVLGSTNLFGGR
                     GTILGTLLGALTIAVLGNGLILIHVSPFYTQIIQGAIMLLAIWMNTRIFSQRRGKKA"
BASE COUNT          658 a         1021 c         1053 g          663 t
ORIGIN      
        1 ggtttgaagt gccgcgcgac atgtcgatcg tcggcttcga cgacatggaa ttcgccagcc
       61 tgctgatgcc gcccatcacc acgctgcggc agtccgcgcg cgaactcggc cgcgcgggcg
      121 tcgccattct gctggccatg ctcgccgccg atcacggcga ggccgcgggc gcagcagccg
      181 cccttgctcc cggtgcggcg ctcggcgcac cggtacatcg cctgccggtg cggctgattg
      241 aacgcgcgtc ggtcgcgccg ccccgcaaga cttagtcttc tttggtattt accctggtct
      301 ttattgaaac ggttgacatc ccaatactct ccatttaact tcaataaacc ggtttacaag
      361 aaggcgggcg caatcttggt tgcgtccttt gaaagtcttt tccactcacg ggacggcgcc
      421 attccacgcg ccgctccgct tactcagaaa agggatttcg atgaacgcac cacattattt
      481 gcgccggctc tccgtcaccc tattcgcgct gtcactcatc ggctcgagcg gcagcgcgtt
      541 cgccgcggac aagaagatcg cactggtgca gatcaaccag gaggcactct tcttcacaca
      601 gatggaccag ggcgcgcagg ccgccgcgaa agcagccggc gtcaaccttg agatctacaa
      661 ctcgaacaac gacccgaacg cgcagaacaa cgccatcgag acgtatatcc agcagaaggt
      721 ggatgcgatt ctagtggtcg cgatcgacgt gaacggcatc aagcccgcca tcagcgaagc
      781 gaagaaagcc ggtattccgg tggtcaccat cgacgccatt gtgaacggcg acaacgccgt
      841 gcaggtgggc gtggacaaca agaaagtcgg cgtggatatc ggcaagtaca cggcgaacta
      901 catcaacaag aatctgggcg gcaaggcgca gatcggcgtg gtgggcgccc tgaattcgtt
      961 catccagaac gtgcggctgg acggtttcaa ggaaggtgtc gcaacgccac cgggtgcaaa
     1021 aatcgtgtcc accgtcgatg gccaaaacgt gcaggatcag gcgcaaactg cagctgaaaa
     1081 cctgatctcg ggcaatccga atctgcagat tgtctatgcg accggggagc ccgcgctgat
     1141 cggtgtggtc gcagcggttt tgtcgcaggg cacgacggat catctgaaga tcgtcggttg
     1201 ggacctgagc gcgcaggcca tcaagggcat cgacgcgggc ttcgtcaccg ccgtcgtgca
     1261 gcaggacccc gaaggcgaag gcaaggcggc agttggcgcc gcgctcaaac tgatcgccaa
     1321 acagccagtc gaaaagaata tctcggtgcc ggtcaccatc gtgacgaaag cgaacgtgga
     1381 caaataccgg gctatcttca agtaagtcca agggaattca atcgagcacc aggtgcgccg
     1441 gacgaggaca tgcagatgaa tagcgttgcc atcactccac gcgtacgcat ggagaacatc
     1501 atcaagagtt acggcgcggt gcaatcgttg cgcggcgtga atctggaagt ggcgcccggc
     1561 gaagtgctgg ggctcgtcgg cgataacggc gcgggcaaat ccacggtatc gaaggtgctg
     1621 gcgggcgcgg ttattcccga cggaggtcgc atcgaaatcg acggcaaccc cgtgcatttc
     1681 acggaccccg gcgacgcccg cgcgcatcac atcgaaatgg tctatcagga cctgtcgctg
     1741 tgcgatacgg tggatgtggc cggcaacctg ttcttcggcc gcgagccgct gcgccgcgtg
     1801 ctcggcattc ccatgctcga ccacgcgaag atgcatcacg acgcccgcga aatgctggat
     1861 ggtctcggca tccgcattcc gaacacgcat ctcgatgtcg aaaatctgtc gggcggccag
     1921 cgtcaggcga ttgcgatttc acgcgctgtg tcgttcgacc ctaaggtgct gatcatggac
     1981 gaaccaaccg cagcgctcgc cgtggctgaa gtcgaagcgg tgctcgaact ggttcagacc
     2041 atcagccagc gtggcgtcag cgtcattctg atcagccatc gcttgcagga cctgttcctc
     2101 gtctgcgacc ggatcagcgt gatgtacgaa ggacagaacg tggcggatcg caagattggc
     2161 gaaacgaatc tggaagaagt cgtcgatctg atcgtcggac gtaaattcaa ggcgcgctcg
     2221 gcgcgcgcgc tctcggagga ctaatcgtgg aaatcgccat gcacaaggac cagagagcgc
     2281 cgcgccgctc gttgcaacat ctgttgggcg gcaatgcggg cgtggccagc attgcggtgt
     2341 tcttcgtctg ctgcgccgtc gctttcagct tcgccaccga taccttcctc accgccggca
     2401 acctgctgaa cgtgctgcgc cagagcgcac cgttgctgat cgtcgcgacc gcgatgacgc
     2461 tcgtgatcac cacggctggc atcgatcttt cgatcggctc gacgcttgcg ctggttggcg
     2521 cactcgccgc cattgcgctg cacgcgggaa tgtcggacag cgtcgtcttg ataggcggcc
     2581 tcctgctcgg cgcgctgatt ggtgcgatca acggctactt catcgcgtgg gccggcatgc
     2641 ccgcgtttat cgtgacgctc ggcaccttgt cggtcgtgcg cggtcttgcg ttgctgatca
     2701 cgcaaggcta ttcggtgccg atcgaaaacg acggctggtt cgtgcaactc ggacgtggtc
     2761 atattctcgg cgtgccgatg cccgccatca tctccgccgt gatcctgttc gccggctggt
     2821 ttacgctgcg tcatatgcgg ttcgggcgtt atgtggtcgg tatcggcacg aacgcggaag
     2881 gtgtgcgccg cgcgggtgta aacgtgcgga tcgtgacgtt gaaggtctac attctgagcg
     2941 gtgcggccgc ggggctcgcg ggtctcatta caacggcgcg gctcggtagc ggttcgtcga
     3001 atcagggcgt cggtttcgaa ctcgcggtga tcgcagccgt cgtgctcggc agcacgaatc
     3061 tgttcggcgg acgcggcacg attctcggta cgctgctcgg cgcgctgacg attgccgtgc
     3121 tcggcaacgg gctgatcctc atccacgtat cgccgttcta tacgcagatc attcaaggcg
     3181 cgatcatgct gctcgccatc tggatgaaca cgcggatctt ttcacagcgg cgcggtaaaa
     3241 aggcttgaac gatgacgtcg atcttccgtc atgtttcgcc gctgccgatt ggcgtggaca
     3301 ccggccatgt gatgaccgtg ctcggtcccc tgcccgtcga gaagatgggc gtcacgctga
     3361 tgcacgagca catcctgctc gatgcctccg gcaag
//
LOCUS       DATASH010001021         3056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5716352,
            whole genome shotgun sequence.
ACCESSION   DATASH010001021 DATASH010000000
VERSION     DATASH010001021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5716352"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(532..918)
                     /locus_tag="VHV99_27080"
     CDS             complement(532..918)
                     /locus_tag="VHV99_27080"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX3639081.1"
                     /translation="MKRYGVEGGKGTGGQHMPFARAVEADGWLFVSGQTPMENGEVIS
                     GGIVEQSHKAIQNVFAILKEAGYGAEHVVRCGVWLDDPRDFASFNKVFREYFGENPPA
                     RACVVSSMVIDCRVEVDCVAYKKPSA"
     gene            complement(1004..2485)
                     /locus_tag="VHV99_27085"
     CDS             complement(1004..2485)
                     /locus_tag="VHV99_27085"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEX3639082.1"
                     /translation="MHSHPEAADTLIVGAQLYDGTGAPPVERDVAIRDGRIAAIGNLS
                     NWLAEEVIEANGRALAPGFIDVHTHDDTHVIRSPQMLPKITQGVTTVIVGNCGISASP
                     VLLKGDPPDPMNLLGERDAFQYPTFASYVEAVNAARPAVNVGALIGHTALRSNQMDRL
                     DRAASAEEIDGMRAQLEEALSNGALGLSSGLAYGSAFSAPTEEVMALAEPLAAAGALY
                     TTHMRTEFDAILDAMDEAYRVGRHAHVPVVISHLKCAGPSNWGRSEEVLNSLEGARRL
                     QPIGCDCYPYNRSSSTLDVKQVTGDIDITITWSEPHPEMAGKLIKDIAAEWGVTQQEA
                     GKRLQPAGAVYHNMSEDDVRRILSHPATMVGSDGLPNDPLPHPRLWGAFPRVLGHYAR
                     DAKLLPLEEAVRKMTSLSARRFGLAQRGEVHIGYHADLVLFDPAKVRDAATFEKPQQA
                     ADGIDAVWVNGVLSYRDGAVTGERAGHFVARGAASKGDAHGAF"
     gene            complement(2489..>3056)
                     /locus_tag="VHV99_27090"
     CDS             complement(2489..>3056)
                     /locus_tag="VHV99_27090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010101707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEX3639083.1"
                     /translation="EVQTALAHNHQLLRQAPFDEAAAALRAARMIYVFGMGGGSTALA
                     DEMRFRLVRLGRPVATYQDGLLQRMVASTVSRECVVLALSTTWRVPEMVENCKIARSY
                     GATVIALTAPASPLAKLADWVIPIVAFETDFIYKPSSSRYAMMMALDVLVTELAVSQG
                     DESRELLRRMKHALDAHRGGGDRQPLGD"
BASE COUNT          636 a         1066 c          830 g          524 t
ORIGIN      
        1 cctgcgtccc tgcatccctg cgtccctgca tccctgcatc cctgcgcctc tgcgcctctg
       61 cgtctctgcg cttgctcgct cgttcttctt cgctcatttg cttcttcggc gcagtcgctt
      121 gtgcgcatcg ccgaggcgaa gccgacggcc cccaccgtgc ccaaacgaag ccggggggtt
      181 ttgataaacc cttccggcga gcacgcaagt gcgaggcggg aagtatgacg gctgtgtcac
      241 tcgcgccgaa tgtgcgaggg cacggattcc agatgcacca ctaccgcgac tgtgcggggg
      301 acccgcttat gcgctagcgg ggtgagccgc tttcttttgc ctacttttct ttgcggcagg
      361 caaagaaaag taggtgcctg ccccgcacag gggcgacgct aatagaccaa taacaattca
      421 aggaaaggcc aaaaacccaa ttcaaggaaa ggccaccacc ataaaaacac agccataaac
      481 cccgcaaaaa agccaaaatc ctatccaccg ccaacggcaa aaagaaccgc cctaagccga
      541 aggcttctta taggcaacgc aatcaacctc cacccgacaa tcaatcacca tcgaagaaac
      601 cacacaagcc cgcgcaggcg gattctcacc aaaatactcc cgaaacacct tattgaaaga
      661 agcaaaatcc cgcggatcat caagccacac gccacagcgg acaacatgct ccgccccata
      721 cccggcttcc ttcaaaatag cgaacacgtt ctgaatcgcc ttatgcgatt gctcaacgat
      781 gccaccgctg atcacctcac cgttttccat cggcgtctgc ccggacacga acagccagcc
      841 atccgcttcg actgcccgag caaagggcat atgttgccca cccgtaccct tgccaccttc
      901 aacgccatat cgcttcatca tccagctcct taaaaagtcc ggcaggcggc gcaagccacg
      961 ccacaaccgc gtgccacatc aatcagaaaa agaaaacaaa ccatcaaaac gcgccgtgcg
     1021 catcaccctt cgacgccgcg ccgcgcgcca caaaatgccc tgcccgctcg ccggtcaccg
     1081 ctccatcgcg ataggacagc acgccattca cccacaccgc atcaatgcca tccgccgcct
     1141 gctgcggctt ttcaaacgtc gccgcatcgc gcaccttggc cggatcgaac aacaccaggt
     1201 ccgcgtgata accaatgtgc acctcgcccc gctgcgccag accaaatcgc cgcgccgaca
     1261 agctggtcat cttgcgcacc gcctcttcga gcggcagcaa cttcgcatcg cgcgcgtaat
     1321 gcccaagcac gcgcggaaac gcgccccaca agcgcggatg cggcaatgga tcgttcggca
     1381 gaccatctga accgaccatc gtcgcgggat gcgaaaggat gcggcgcacg tcgtcctcgg
     1441 acatgttgtg atacaccgcg ccagccggct gcaagcgctt gcccgcctcc tgctgcgtga
     1501 cgccccactc ggcggcaatg tccttgatca gcttgcccgc catctcggga tgcggctctg
     1561 accatgtgat cgtgatgtcg atgtcgccgg tcacctgctt cacgtcgagc gtcgacgaac
     1621 tgcggttata cggatagcaa tcgcaaccga tcggctgcag acgacgcgcg ccttcgagcg
     1681 agttcagcac ctcctcactg cgcccccagt tcgacggccc cgcgcacttc aaatgcgaaa
     1741 tcaccaccgg cacatgcgcg tgacggccca cacggtaagc ctcgtccatc gcgtcgagaa
     1801 tcgcgtcgaa ttcggtgcgc atgtgcgtgg tgtacaacgc gccggcagcg gcgagcggct
     1861 cggccagcgc catcacttcc tcggtcggcg cggagaaagc ggagccatat gcgagtccgg
     1921 agctcaggcc cagcgcaccg ttcgacaacg cctcttcgag ctgcgcacgc atcccgtcga
     1981 tttcttccgc gctcgccgcg cgatcgagcc ggtccatctg attgctgcgc aacgccgtgt
     2041 gcccaatcag cgcgccgaca ttcaccgctg ggcgcgcggc gttcaccgct tcaacgtacg
     2101 aagcgaaagt cgggtattga aacgcgtcgc gctcgccaag caggttcatc ggatcaggcg
     2161 gatcgccctt caacaaaacc ggcgacgcgc tgatcccgca attgccgacg atcaccgtcg
     2221 tcacaccctg agtaatcttc ggcagcattt gcggcgaacg gatcacgtgc gtgtcgtcat
     2281 gcgtatgaac gtcgatgaag cccggcgcca gcgcgcggcc attggcctcg atcacttctt
     2341 cggcgagcca gttcgacaga ttgccgatcg cggcaatgcg gccgtcgcgg atcgccacgt
     2401 cgcgttcgac cggcggcgcg cccgtgccgt cgtaaagttg cgcgccgaca atcagcgtgt
     2461 cggcggcttc gggatgcgaa tgcatgaatc agtctcctaa cggttgtcga tcgccgccgc
     2521 cacggtgcgc gtcgagcgcg tgcttcatgc ggcgcagcaa ttcgcggctc tcgtcaccct
     2581 gactgacggc gagttcggtg acgagcacat cgagcgccat catcatcgcg tagcgcgacg
     2641 acgacggctt gtaaatgaaa tcggtttcga aggcgacgat cgggatcacc caatcggcca
     2701 gtttggcaag cggcgacgcc ggtgcggtca gcgcgatcac ggtcgcgccg tagctgcgcg
     2761 cgatcttgca gttctcgacc atctcgggca cgcgccaggt agtggatagc gccagcacca
     2821 cgcactcgcg cgacacggtg ctcgccacca tgcgctgcaa caagccgtcc tgataggtcg
     2881 caaccggccg gccgagacgc acgagcctga agcgcatctc gtcggccaat gcggtcgaac
     2941 cgccgcccat gccgaatacg tagatcatcc gcgcggcgcg caacgcggcc gccgcttcgt
     3001 cgaacggcgc ctggcgtagc aactggtggt tatgggcaag cgcggtctgt acttcg
//
LOCUS       DATASH010001022         2666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1560278,
            whole genome shotgun sequence.
ACCESSION   DATASH010001022 DATASH010000000
VERSION     DATASH010001022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1560278"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="VHV99_27095"
     CDS             <1..542
                     /locus_tag="VHV99_27095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX3639084.1"
                     /translation="AGLGLAIGTAWGGLLGHFAAGIFMQILRPFKVGDFVTAGGVTGT
                     VHELGLFGTTIVTPDNVTTIVGNNKIFSDTISNYSVLPVRRVELTAKIANGVDPTDAM
                     NRLKAAVTQIPNVAESPAPDIEVLSFTPEGPLLCVRPYTNNEHYWQVYFDTNRAIVQT
                     FKEAGYPTPETPLAPRAVT"
     gene            complement(708..1379)
                     /locus_tag="VHV99_27100"
     CDS             complement(708..1379)
                     /locus_tag="VHV99_27100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX3639085.1"
                     /translation="MDTLLHFVNLVLHIDKFLGDFIHVYGTWVYAVLFLIVFCETGLV
                     VLPFLPGDSLLFIGGAFCASGEMNLGLLIVLLLVAAVAGNTVNYMIGRAIGPRVFNSH
                     IPFLERFLDRAALQKTHNFYEKHGGKTIVLARFIPVVRTFAPFVAGASEMTVSRFQLF
                     NILGALLWVLLLVLLGYFFGNIPFIRQYLNVIVLVGIGAAVVPVVLGALWKMTRKNAS
                     KAGTR"
     gene            1720..>2666
                     /gene="mutL"
                     /locus_tag="VHV99_27105"
     CDS             1720..>2666
                     /gene="mutL"
                     /locus_tag="VHV99_27105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEX3639086.1"
                     /translation="MSEFSETPAGTTDAPAVSAAAPVPRPLRAIQPLPDQLISQIAAG
                     EVVERPASVVKELLENALDAGAQTLRILLDEGGVKRISITDDGCGIPENELALALMRH
                     ATSKIRSLAELEAVATLGFRGEALASIASVAQMSITSRTAEAPHAVRVDAQTGVLSPA
                     AGTQGTTIEVRELYFNTPARRKFLKSEQTELGHCLEQIRRAALARPDVAISVLHNGKA
                     VEHWNASEPPARVAKILGETFATAHLPLDESAGPLAVYGCAGLPTASRGRADQQYFFV
                     NGRFVRDKLLTHAVRAAYEDVLHGDRYPSYVLFLDLPPEA"
BASE COUNT          556 a          859 c          810 g          441 t
ORIGIN      
        1 tcgcgggcct cggcctcgcg atcggcaccg cgtggggcgg cctgctcggg cacttcgcgg
       61 cgggcatctt catgcagatc ctgcggccgt tcaaggtcgg cgatttcgtg acggcgggcg
      121 gcgtgaccgg caccgtccac gaactgggcc tgttcggcac gacgatcgtg acgcccgaca
      181 acgtgacgac catcgtcggc aacaacaaga tcttctccga cacgatctcg aactacagcg
      241 tactgccggt acggcgtgtc gaactgacag cgaagatcgc caacggcgtc gacccgacgg
      301 atgcaatgaa ccggctgaag gcggccgtca cgcagattcc gaacgtggcc gaaagccccg
      361 cgccggatat cgaagtgctg agcttcacgc cggagggacc gttgttatgc gtgcggcctt
      421 atacgaacaa tgagcattat tggcaagtct acttcgacac caatcgcgcg atcgttcaga
      481 ccttcaagga agccggctat ccgacaccgg aaacgccgct tgcgccgcgc gcagtgacgt
      541 gaacgcgacc gaagtcagct acacgaatga tcgagttcgc ggcgccagga tgacaaattt
      601 gctcactact tccgcgatcc ggctcagcgg aatcaggacc cgaaatgaaa acggcgtcgc
      661 gagattcgcg acgccgtttg ttcgtgtgct ccatgcgacg tgaagcgtca gcgggtgccc
      721 gccttggacg cgttcttgcg cgtcatcttc cataacgcgc ctaacacaac cggtaccacg
      781 gccgcgccga tacccaccag cacgatcaca ttcagatact gccggatgaa cggaatgttg
      841 ccgaagaagt aaccgagcaa caccagcagc aacacccaca acaacgcacc gagaatgttg
      901 aagagctgga atcggctcac cgtcatctcc gatgcaccgg caacgaacgg cgcgaaggta
      961 cgcaccaccg gaatgaaacg cgccaggacg atggtcttgc cgccatgctt ttcatagaag
     1021 ttatgagtct tctgcagcgc ggcgcggtcg aggaagcgct caaggaaagg gatgtgcgaa
     1081 ttgaacaccc gtggcccaat ggcccgcccg atcatgtagt tgaccgtatt gccggcaacc
     1141 gccgcaacga gcaacagcac gatcaggagg ccgagattca tttcaccgct cgcgcaaaaa
     1201 gcgccgccga tgaacagcag ggaatcgccc gggagaaacg gcagcacgac cagtccggtt
     1261 tcgcagaaca cgatcagaaa caggaccgcg tagacccagg tgccgtacac gtgaatgaag
     1321 tcacccagaa acttgtcgat gtgcaagaca aggttgacga agtgtagcaa cgtgtccaag
     1381 aagcgtcctt tattgagcga atgcaagcgt ggcgggagtg gccgcgtcag cagccttgca
     1441 ggcggcggcc ggcgagcgcg tgctcgccgc cgcaaattga ccgcgtcatg ataccgaaag
     1501 tgcccggttg cgattaaaaa tctgccgggc atcgacactt tcggggctgt gcggcggttc
     1561 tgcggtccaa actgaacgca tgccgcacag gtcgatcgca tcgccaactg ggtgccatgt
     1621 ctgccgttct ggttcagcgg ccgccggccg caagaccgcg ccgacccata gcgtcgaccc
     1681 acatcgagcc ggctcgcgcc gaatcgctat aatttcgcca tgtcagaatt ctccgaaaca
     1741 ccagccggca ccaccgacgc gcccgcggtt tccgccgccg cgcccgtccc acgcccgttg
     1801 cgggcgatcc agccccttcc cgatcagctg atcagccaga ttgccgctgg cgaagtggtc
     1861 gagcggccgg cctcggtggt taaggaattg ctggagaatg cgctcgacgc cggcgcgcag
     1921 acgctgcgta tcctgctcga cgaaggcggt gtcaaacgca tttcgatcac cgacgacggt
     1981 tgcggcatcc ccgaaaacga actcgcgctc gcactgatgc gtcacgcgac cagcaagatt
     2041 cgctcgcttg ccgaactgga agcggtcgcg acgctggggt ttcgcggtga ggcgctggcg
     2101 tcgattgcgt cggtcgcgca gatgagcatc acgagccgta ccgccgaggc gccgcatgcc
     2161 gtgcgcgtcg acgcgcaaac cggtgtgctg agccccgccg ccggcaccca gggcaccacg
     2221 atcgaagtcc gcgagctgta cttcaacacg cccgcgcgcc gcaaattcct gaaaagcgag
     2281 cagaccgaac tcggtcactg cctcgaacag attcgccgcg ctgcgctggc acggccggat
     2341 gtggcgattt cggtgctgca taacggcaag gcggtcgaac actggaacgc tagcgagccg
     2401 cccgcgcggg tcgcgaagat tcttggcgaa acctttgcga cggcgcattt gccgctcgac
     2461 gaatccgccg gaccgctcgc cgtctacggt tgcgccggtc tgccgaccgc gagccgcggg
     2521 cgcgccgatc agcagtattt cttcgtcaat ggccggttcg tgcgcgacaa gctgctcacg
     2581 cacgcggtgc gcgccgctta cgaagacgtg ctgcacggcg accgctatcc gtcctacgtg
     2641 ctgtttctcg atctgccgcc agaagc
//
LOCUS       DATASH010001023         3657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3933637,
            whole genome shotgun sequence.
ACCESSION   DATASH010001023 DATASH010000000
VERSION     DATASH010001023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3933637"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..84
                     /locus_tag="VHV99_27110"
     CDS             <1..84
                     /locus_tag="VHV99_27110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014973250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX3639087.1"
                     /translation="CLCYLALVELTSRVGMWAETRIARFMA"
     gene            104..973
                     /locus_tag="VHV99_27115"
     CDS             104..973
                     /locus_tag="VHV99_27115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012404358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX3639088.1"
                     /translation="MNAIADMPSSTSPAVFDTPSGAPLIEVRDLKKRFGVVEVLRGVD
                     LEIARSEVVCIIGPSGSGKSTLLRCLAALETYDHGDVRIEGELLGYSERNGKRVRASQ
                     SEINRVRRNVGMVFQQFNLWPHMTALGNVMEALLRVRQMSRDEARRRANAMLDTVGLA
                     HKGDAYPSKLSGGQQQRVAIARALAMEPHLMLFDEPTSALDPELVGEVLQVMKQLARD
                     GMTMAVVTHEMGFAAQVADKVMFIDQGRIAVQGKPREVFHDAGQPRLRQFLQNYFDRN
                     AFWARGPEDAEPV"
     gene            1119..1916
                     /gene="hutC"
                     /locus_tag="VHV99_27120"
     CDS             1119..1916
                     /gene="hutC"
                     /locus_tag="VHV99_27120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine utilization repressor"
                     /protein_id="HEX3639089.1"
                     /translation="MTSPVRSNAHDTPAAAMKTARVATDAPAARYEQVKNHIRQIIES
                     GARQAGDRLPSELDLVATLGLSRMTVNRALRELADEGLVTRVSGVGTFVAQSKPQSTL
                     LMIAHIGDEIRSRGHEYSYDTVLSQRETAPVTVSNALGLAPGTSVFHVICVHRENGLP
                     VQLEDRYVNPVTAPDFLKQDFSTTRPSEYLFETVPAHDVEHVVDAGLPTRAEAEWLEI
                     RADEPCLTLMRRTWTSGVAVTVARFVHPGSRYRLGCRFTPDLAQRQG"
     gene            complement(1959..2642)
                     /gene="hutC"
                     /locus_tag="VHV99_27125"
     CDS             complement(1959..2642)
                     /gene="hutC"
                     /locus_tag="VHV99_27125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine utilization repressor"
                     /protein_id="HEX3639090.1"
                     /translation="MPAYEQIKRYVIRRISEGDWKPGGLIPSETELVKEFGVARMTVS
                     RALRELTTERVLTRVQGSGTFVAPQRYESTVLEIRNIADEIAARGHRHSARVLTLEPS
                     DDPHALEALGLAGGPAFHSCIVHSEEGEPIQYEDRYVNPKVFPDYLQQDFTVETPNHY
                     MVRLAPIQRAEFRIYAQKPDAYVRRHLMMDIGEPCLQLWRRTWVGDDVATSVQLWHPA
                     SRFHLAGNV"
     gene            complement(2741..>3657)
                     /locus_tag="VHV99_27130"
     CDS             complement(2741..>3657)
                     /locus_tag="VHV99_27130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017773644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HEX3639091.1"
                     /translation="GLNLVGERMRRALADSGILAAVVGQRTQDPLSMRTIPHVHGAAR
                     DVLTATADVVNRELASITDNPIVAGTPEAPRVYSQAHAVGASIALAMDSLATAIAQVA
                     AMVERRLDRLVNPLVSGLPAFLAEPGGTCSGFMIAQYTAASLVAQNRRLAMPASLDGG
                     ITSGLQEDHLCHATPAALKALEIVDNAGRIVAIELLAAAQAYDLQSLDAPRAPHTDAL
                     WQRVRRVVPTYRDDRPLADDMAIAFRMIADGMPPPLPNPGKIRPGPDASNGGAGLAAW
                     ASVTSLAAAGMLGADPAANDGGAAAHAG"
BASE COUNT          616 a         1189 c         1198 g          654 t
ORIGIN      
        1 tgcctgtgtt acctcgcgct ggtcgaactg acttcgcgcg tggggatgtg ggccgaaacc
       61 cgcatcgcgc gcttcatggc atgaacggaa tccgcaccat tttatgaacg ctatcgccga
      121 catgccgtcc tccacgtccc ctgctgtttt cgatacgccg tccggcgcgc cattgatcga
      181 agtgcgcgac ctgaagaaac gctttggcgt tgttgaagtg ctacgcggcg tcgatctcga
      241 gatcgcccgt tccgaagtgg tctgcatcat cggtccgtcg ggctcgggca agagcacgct
      301 gctgcgctgc ctcgccgcgc tcgaaaccta cgatcacggt gacgtgcgca tcgaaggcga
      361 attgctcggc tatagcgagc gcaatggaaa acgcgtgcga gcatcgcaaa gcgaaatcaa
      421 tcgcgtgcgg cgcaacgtcg gcatggtgtt tcagcagttc aatctgtggc cgcatatgac
      481 ggcgctcggc aatgtgatgg aagccttgct gcgcgtgcgc cagatgtcgc gtgacgaagc
      541 gcgccgccgc gccaatgcga tgctggacac cgtcggcctt gcgcacaaag gcgatgcgta
      601 cccgtcgaaa ctctcgggcg gccagcagca gcgcgtggcg attgcacgcg cgctggcgat
      661 ggagcctcac ctcatgctgt tcgacgagcc gacctcggcg cttgaccccg agctggtcgg
      721 cgaagtgctg caggtgatga agcagctcgc gcgcgacggc atgacgatgg ccgtcgtcac
      781 gcacgaaatg ggcttcgccg cccaggtcgc cgacaaggtg atgttcatcg accaggggcg
      841 catcgccgtg caaggcaagc cgcgcgaagt ctttcacgat gccgggcagc cgcgcttgcg
      901 gcagtttctg caaaactact tcgaccgcaa tgctttctgg gcgcgcgggc ctgaggatgc
      961 ggagccggta tgaagaagct gcgcaggcgc tcgcagggtt cgttcggctt gtgcggttcg
     1021 ctgggcttgc gcggttcgcc gggcttgcgc gaatcacctg gttcgtgcgg ttcgtgcggt
     1081 tcgtgcggtt cgtgcggact gtagcgtaaa ggccgccgat gacctccccc gttcgcagca
     1141 acgctcacga cacgcccgcc gcagcgatga aaaccgcgcg cgtggcgacc gacgcaccgg
     1201 ccgcccgcta cgaacaggtc aagaaccaca tccgccagat catcgaatcg ggcgcgcggc
     1261 aagcgggcga tcgcctgccg tccgagctgg acctcgtcgc gaccttaggc ctgtcacgca
     1321 tgacggtgaa tcgcgcgctg cgcgaactcg ccgatgaagg tctcgtcacg cgcgtgtccg
     1381 gcgtcggcac ctttgtcgcg caatccaagc cgcaatccac gctgctgatg atcgcccata
     1441 tcggcgacga gatccgctcg cgcggtcacg aatatagcta cgacaccgtc ctctcgcaac
     1501 gcgaaaccgc gccggtgacg gtgtcgaacg cgttagggct ggcgcccggc acgtccgtgt
     1561 tccatgtgat ttgcgtgcat cgcgagaacg gcttgccggt gcagctcgaa gaccgctacg
     1621 tcaatccggt aaccgcaccg gacttcctca agcaggattt ctccaccacg aggccgtccg
     1681 agtatctgtt cgaaaccgtg ccggcgcacg acgtcgaaca tgtcgtcgac gccggtttgc
     1741 cgacgcgcgc cgaagccgag tggctggaaa ttcgcgccga tgagccgtgc ctgacactga
     1801 tgcgccgcac atggacgagc ggcgttgcgg tcacagtcgc acgtttcgtg caccccggtt
     1861 cgcgatatcg gctgggttgc cggttcacgc cggatctggc gcagcgccaa ggctaggtta
     1921 gcggtgcgat cgccacgccc gcgcgcgcgt cttgccgctc acacattgcc tgccagatga
     1981 aaacgcgacg ccggatgcca caactgcacc gacgtcgcca catcatcacc gacccatgtg
     2041 cggcgccata gctgcaaaca gggctcgccg atatccatca tcagatgccg ccgcacatac
     2101 gcatcgggct tttgcgcata gatgcggaac tccgcgcgct ggatcggcgc gagcctcacc
     2161 atgtagtgat tcggcgtctc gacggtgaag tcctgttgca ggtagtcggg gaacactttc
     2221 ggattgacgt agcgatcttc gtactggatc ggctcgccct cttcgctgtg cacgatgcac
     2281 gaatgaaacg cgggaccgcc ggcgaggccg agcgcttcga gcgcgtgcgg atcgtcactc
     2341 ggttcgagcg tcagcacacg cgctgaatga cgatggccgc gcgcggcgat ttcgtcggcg
     2401 atattgcgga tttccagcac cgtcgattcg tagcgctgcg gcgcgacgaa ggtgccggaa
     2461 ccttgcacac gcgtcaggac gcgttcggtg gtcagctcgc gcagggcgcg cgataccgtc
     2521 atgcgcgcga cgccgaattc cttgaccagc tctgtttcag aggggatcag gccgcccggc
     2581 ttccagtccc cctcgctaat gcgcctgatc acgtagcgct tgatctgctc gtaggcgggc
     2641 atcgccttgg cgggcgtctc atgggtgcga tccgggcggt cctgcgtgtc tgcggtttgc
     2701 gcgtttgtaa tagtgttcac gtcgacctcg tgggtgttct tcaacctgcg tgggcagccg
     2761 ccccgccatc gttggccgcc gggtcggcgc cgagcattcc cgcggcagca aggctcgtca
     2821 cgctcgccca tgcggcgaga ccggcgccgc cgtttgaagc atccggcccg ggccgaatct
     2881 tacccgggtt tggcagcggc ggcggcattc catcggcaat catgcgaaac gcgattgcca
     2941 tgtcgtccgc gagcgggcgg tcgtcacgat aggtcggcac gacgcggcgc acgcgctgcc
     3001 agagtgcgtc cgtgtgcggt gcacgcggtg cgtcgaggct ctgcaggtcg taggcctgcg
     3061 ccgccgccag caattcgatc gcgacgatgc gcccggcgtt gtcgacaatc tccaacgctt
     3121 tcagcgcggc gggcgtggca tggcacaagt ggtcctcctg caggccggac gtgatcccgc
     3181 catcgagcga cgcgggcatc gccagacgcc ggttctgtgc gaccagcgag gcggccgtgt
     3241 actgcgcgat catgaaaccg gaacaggtac cgcccggctc cgcgagaaac gccggcaaac
     3301 cgctcacgag tggattgacg aggcggtcga ggcgccgctc gaccatcgcc gcaacttgcg
     3361 cgatcgcggt ggcgaggctg tccatcgcga gagcaatcga cgcccccact gcatgcgcct
     3421 gcgaataaac gcgcggcgct tccggcgtgc cggcgacgat cgggttgtcg gtaatcgacg
     3481 cgagctcgcg attgaccaca tcggcggtcg cggtgagcac gtcgcgcgcg gcgccatgga
     3541 catgcggaat ggtccgcatg ctcagcggat cttgcgtgcg ctggccgacc accgcggcga
     3601 gaatgccgct atcggcgagc gcgcgacgca tccgttcgcc gaccaggttc aggcccg
//
LOCUS       DATASH010001024         4630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2943881,
            whole genome shotgun sequence.
ACCESSION   DATASH010001024 DATASH010000000
VERSION     DATASH010001024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4630
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2943881"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VHV99_27135"
     CDS             complement(<1..275)
                     /locus_tag="VHV99_27135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018435552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HEX3639092.1"
                     /translation="MEGFITDWLNLALRWFHVIVAIAWIGESFYFVALDNSLKPPTDP
                     NQRKRGVFGELWHVHGGGFYNMQKYTVAPPEMPDDLHWSKWPSYTTWL"
     gene            complement(580..933)
                     /gene="uraH"
                     /locus_tag="VHV99_27140"
     CDS             complement(580..933)
                     /gene="uraH"
                     /locus_tag="VHV99_27140"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HEX3639093.1"
                     /translation="MGKLTTHVLDTANGRPGAGIKVELFALSGDTRRALKTTTTNNDG
                     RCDQPLLEGDALVAGEYELVFGAGDYFASIGTKVPEPRFVDRVVLRFGVADAGAHYHV
                     PLLVSPWSYSTYRGS"
     gene            1446..2843
                     /locus_tag="VHV99_27145"
     CDS             1446..2843
                     /locus_tag="VHV99_27145"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HEX3639094.1"
                     /translation="MTMEQAAQRPNKPKRTMLVKHADVLVTMDGARRELRDGGMYIED
                     NRIVAVGPTAELPQTADEVLDMRGHLVIPGLVNTHHHMYQSLTRAIPAAQNAELFGWL
                     TNLYKVWANLTPEMIEVSTLTAMAELLLSGCTTSSDHLYIYPNGSRLDDSIVAAGRIG
                     MRFHAARGSMSVGKKDGGLPPDSVVEREADILKDTQRLIETYNDEGRYAMLRVVVAPC
                     SPFSVSRDLMRESAVMARHYGVSLHTHLAENVNDIAYSREKFGMTPAEYAEDLGWVGH
                     DVWHAHCVQLDDAGIELFARTGTGVAHCPCSNMRLASGIAPVKRMRLAGVPVGLGVDG
                     SASNDGAQMVAEVRQALLLQRVGFGPDAMTAREALEIATLGGAKVLNRDDIGALAPGM
                     AADFVSFDLRQPLFAGALHDPVAALVFCAPSQVSHSVISGKVVVKDGQLTTLELGPVI
                     EQHNRLAKTLYEAAA"
     gene            complement(2948..3880)
                     /locus_tag="VHV99_27150"
     CDS             complement(2948..3880)
                     /locus_tag="VHV99_27150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX3639095.1"
                     /translation="MSQQREAIDTYLLRVLHTLLMERSVTRAAVKLNQSQPAISAALR
                     RLRDITGDPLLVRGKSGMVPTEYGLRLLEPVQNALREIERIKFQQHNFDPATSIRCYR
                     IGCPDYLNVLFVPTVVERFRQAAPNATLEFHSLGPAFDYELALEDGKLDIVVGNWPEP
                     PEQLHLSNLFVDQIVCLMSNAHPFAKRGGLTLDQYLNAPHLAPTPYSVGQRGAIDVHL
                     ARERLKRHVVVTLPYFNLAPYVLIKSDLIFTTTRLFADYYAKFLPLTVVPAPLDFPPM
                     QYYQLWHERVHYSDEVRWLRSLVAEATRTLIEKP"
     gene            4131..>4630
                     /locus_tag="VHV99_27155"
     CDS             4131..>4630
                     /locus_tag="VHV99_27155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3639096.1"
                     /translation="MSNSYSDGAAAQPASRPEQAAPRLMLQGITKQYPAVRANDDVTL
                     IVAPGEIHAVLGENGAGKSTLMKIIYGAVRPDAGEIRWEGQPVEIASPAAARKLGIGM
                     VFQHFSLFETLTVGENIALALDEPFDLKTLARRIREVSASVFRSNGSSSASAMFSPTV
                     SVSNSE"
BASE COUNT          916 a         1314 c         1542 g          858 t
ORIGIN      
        1 agccacgtgg tgtacgacgg ccacttggac cagtgcaggt cgtccggcat ttccggcggc
       61 gcgacggtgt acttctgcat gttgtagaag ccgccgccgt gcacgtgcca cagctcgccg
      121 aacacgccgc gcttgcgctg attcgggtcc gtaggcggtt tcagactgtt gtcgagtgcg
      181 acgaaataga acgattcgcc gatccacgcg atggcgacga tgacgtggaa ccagcgcaat
      241 gccaggttca gccagtcggt gataaagcct tccatgaaac tcctccactt ctgattcgtt
      301 gtacgcgcaa cgatgcttgt gccttggcta tgccttcggc gacccggctg acgggtcgtc
      361 ggcatgcacg gtgtcgctgc gcagactgcg gggtgagaga cgcaatgcgc tcagttcgca
      421 cggcgccgtt ttttattgga acctggggtc gagtcgctcg cattcgattg cgcttcgcgg
      481 gtgtgtctcc acgcatctgc gcagtgcaat cgggcgcgat cgaaaacgca acgtcaacct
      541 cgttctcctc caacgcctat cgaaaaacgc tctgtgcgct taactgcctc gataggtact
      601 gtacgaccac ggcgacacca gcagcggcac gtgatagtgc gcaccggcat cggccacgcc
      661 gaaacgcagc acgacgcggt cgacgaaacg cggctcgggc accttcgtgc cgatcgaagc
      721 aaagtaatcg ccggcgccga acacaagttc gtattcgccc gcgaccaatg cgtcgccttc
      781 gagcagcggc tggtcacagc ggccgtcgtt attggtggtg gtggttttga gcgcgcggcg
      841 ggtgtcgccg gagagcgcga agagttcgac cttgatgccc gcgccaggac ggccgttcgc
      901 ggtgtcgagc acatgggtag tgagctttcc cattcgcaat tttccttatg tgaatgcgag
      961 gtttcggcgg cggcccgatc cagaatacgt tgcggcggat cgaggctcca cgtcagtggc
     1021 tacatacccg tcggcgaatt gaagcgagcg ccgtggcagc cattgtaaaa aggatgttca
     1081 cgtcagcgcg cctgagatag gaaagataat agctggcgca tgacagacag ccggctgaaa
     1141 gccgcaacgc gtcggtttgt tcgattgttg cagtgcattt aacctatcgg cggatcgtac
     1201 cggtgccaaa aaggtccact atatcggcag cgtgaacttg ggtatctcat tggcgcgagt
     1261 tgtcagcatt attttgaccg tcgaaggtta cgcctgtgct gcggcagcgc ggcacatccc
     1321 gaagacggcg ataacacgct gggtaaccgg gccaacggcg ttcaacggga cgcggcggca
     1381 cggttggcgt gccgtcacat cgcgttcgaa cggctttgca ctgaagcaat gaagcggaga
     1441 agcgaatgac gatggagcag gcggcccaaa ggccgaacaa gcctaaaaga acgatgctgg
     1501 tcaagcacgc ggacgtgctg gtcacgatgg acggcgcccg gcgcgaactg cgcgacggcg
     1561 gtatgtacat cgaggacaac cgcatcgtcg cggtcggacc gacggcggaa ttgccgcaaa
     1621 cggccgacga agtactggac atgcgcggtc acctggtgat tccagggctg gtgaacacgc
     1681 accaccatat gtatcagagc ctgacgcgcg ctattccggc tgcgcagaac gccgagttgt
     1741 tcggctggct gaccaacctc tacaaggtgt gggcgaacct tacaccggag atgatcgaag
     1801 tctcgacctt gacggcgatg gccgagttgt tgctgtccgg ttgcacgacc tcgagcgacc
     1861 atttgtatat ctacccgaac ggcagccgtc tcgacgacag catcgtcgcg gcagggcgga
     1921 tcggcatgcg ttttcacgca gcgcgcggca gcatgagcgt ggggaagaag gacggtggcc
     1981 taccaccgga ttcggtggtc gaacgtgaag cggacatcct gaaggacacg cagcggctga
     2041 tcgagacgta taacgacgaa ggccgttacg cgatgctgcg cgtggtggtg gcgccgtgct
     2101 cgccgttctc ggtgagccgc gatctgatgc gcgaatcggc ggtaatggcg cggcattacg
     2161 gggtgtcgct gcacacgcac ctggcggaga acgtcaacga cattgcgtat agccgcgaga
     2221 agttcggcat gacgccggcc gagtatgcgg aagatctcgg ctgggtcggt cacgatgtat
     2281 ggcacgcgca ctgtgtgcaa ctcgacgacg cgggcatcga actgttcgct cgcacgggca
     2341 ccggcgtggc acattgcccg tgctcgaaca tgcggctcgc ctcggggatt gcgccggtca
     2401 aacggatgcg tcttgccggc gtgccggtgg gcctgggcgt ggacggctcg gcgtcgaacg
     2461 acggtgcgca aatggtcgcc gaagtgcgcc aggcgctgct gctgcagcgg gtcggcttcg
     2521 gaccggacgc gatgaccgcg cgtgaagccc tggaaatcgc cacactaggc ggcgccaagg
     2581 tgttgaatcg cgatgacatt ggcgcgctgg caccgggcat ggccgcggac ttcgtgtcgt
     2641 tcgatctgcg tcagccgctg tttgccggcg ccttgcatga tccggtcgcg gcgttggtgt
     2701 tctgcgcgcc gtcgcaggtg agccatagcg tgatcagcgg caaggtggtg gtgaaagacg
     2761 gacagttgac gacgctggaa ctcggaccgg tcatcgagca gcacaaccgg ttggcgaaga
     2821 cgttgtatga agcggcggcg tagcaaagga gaaagggcgg agtaatcgcg cactggaagc
     2881 ggcactgtta tcggcatcca tcagagcgga tgccgataac aggggccgat gagccccagc
     2941 gcaaggatca aggcttttcg atcaacgtcc tggtggcttc cgccaccaga ctgcgcagcc
     3001 agcgcacttc gtcggaataa tgcacgcgct cgtgccacag ctggtagtac tgcatcggcg
     3061 ggaaatcgag cggggcgggc accacggtca gcggcaggaa cttggcgtag taatcggcaa
     3121 acaggcgcgt cgtggtgaag atcagatcgg acttgatcag gacgtagggc gccagattga
     3181 agtacggcaa cgtaacgaca acatgacgtt ttaaccgctc gcgcgcgaga tgcacgtcga
     3241 tcgcgccgcg ttggcccacc gagtaaggag taggcgcaag atgcggtgca ttcagatact
     3301 ggtcgagcgt cagcccgccg cgtttggcga acggatgcgc gttgctcatc aggcagacga
     3361 tctgatcgac gaacagattc gacaggtgca gttgttccgg cggctccggc cagttgccga
     3421 ccacgatgtc gagcttgccg tcctcgagcg cgagttcgta gtcgaaggcg ggcccgagcg
     3481 agtgaaattc gagcgtcgcg ttcggcgcgg cctgacgaaa acgttcgacc accgtcggca
     3541 cgaacaggac gttcaggtag tccgggcagc cgatccggta acaacggatc gaggtggccg
     3601 gatcgaagtt gtgctgctgg aacttgatgc gttcgatctc gcgcagcgcg ttttgcaccg
     3661 gttcgagcag gcgcaggccg tactcggtcg gcaccatgcc ggacttgccg cgcaccagca
     3721 gcgggtcgcc ggtgatgtcg cgcaaacggc gcagtgccgc gctgatagca ggttgcgatt
     3781 gattcagttt gacggccgcg cgcgtgacgc tgcgctccat cagcagggtg tgcaagacgc
     3841 gtaacaggta tgtatcgatc gcctcgcgtt gctgactcat gacttctccg aaatatatgt
     3901 tctggctgat cgagtgggcg tgggtgtata tgacttttaa tatgaacaca aatccacgtc
     3961 aagagtggga tacccgcaga gcacgctgcg gcgtgggttt gcggggaatt ttggcggctc
     4021 gactgactgg gtcgaattag gtattgtcat ccggttgtgg tgacggatcg cttgctgacg
     4081 gggcgggcgg acgcgccgcg gcggcagtct ccgacgtact acggaatcac atgagcaact
     4141 cttatagcga cggcgccgcc gcgcagccgg caagcaggcc ggaacaggcg gcgccccggt
     4201 tgatgcttca gggcatcacc aaacaatatc cggccgtgcg cgccaacgac gacgtcacgt
     4261 tgatcgtcgc gccgggtgaa atccatgccg tgctcggcga aaacggcgcc ggcaaaagca
     4321 ccttgatgaa aatcatctac ggcgcggtgc ggcccgacgc gggcgagatc cgctgggaag
     4381 gtcagccggt cgagatcgcc agcccggcgg cggcccgcaa gctcggcatc ggcatggtgt
     4441 tccagcattt ttcgctgttc gagacgctca cggtcggcga aaacatcgcg ctcgcgctcg
     4501 acgaaccgtt cgatctgaag acgcttgcga ggcgtatccg cgaagtctcc gcaagcgtct
     4561 tcagatcgaa cggttcgtcg agcgcgagcg cgatgttttc gccgaccgtg agtgtctcga
     4621 acagcgaaaa
//
LOCUS       DATASH010001025         2507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2943962,
            whole genome shotgun sequence.
ACCESSION   DATASH010001025 DATASH010000000
VERSION     DATASH010001025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2943962"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="VHV99_27160"
     CDS             <1..753
                     /locus_tag="VHV99_27160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEX3639097.1"
                     /translation="LDKIGIVNPFDNAGWRELIERVVAANEADAGLRADQDAIAYVQI
                     TRGVAKRGHAFPAGVQPTVFVMVTPVNLPDAAQRAQGVRCVTAEDRRWLNCDIKSVSL
                     LGNVLMAQYAAENDAFETIQFRDGMLTEGSSSNIWMAKDGVLYAPPRSHKILEGIRYG
                     LIEELTSECGIRFEAREIAEAELRTADEVLVSSATKEVLPVTQIDGQPVGDGKPGAVF
                     AALYAAYQRAKAKEAQEAQQGVESESRERIIA"
     gene            725..994
                     /locus_tag="VHV99_27165"
     CDS             725..994
                     /locus_tag="VHV99_27165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein"
                     /protein_id="HEX3639098.1"
                     /translation="MNPAKESLLEFPCDFPIKIMGKSHPEFAETIVTVVRQFDNEVDP
                     ARVETRPSSGGNYTGLTVTVRATSQAQLDDIYRALTGHPMVKVVL"
     gene            complement(1066..2034)
                     /locus_tag="VHV99_27170"
     CDS             complement(1066..2034)
                     /locus_tag="VHV99_27170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HEX3639099.1"
                     /translation="MDLRQLPPLNAIKAFEAAARHESFSRAADELFVTHGAVSHQIRA
                     LEAELGITLFARDGKRVRLTDTGRRYATRVRTALMALADATREVRAGDRERRLVVSML
                     TSFAARWVTPRIGSFIEAHPQWDLELLSTNALTDFARDDVDVAIRFGYGKYSGLHAEL
                     LLEEIFFPACAPDFNGGKLPQTPAELAKAPLLRSDDELWRPWFDAAGLADWPEPKRGV
                     LYQDSSNLLQAAIDGQGIALTRRSLAMHEIAAGRLVRLFDVDGPSPWQYYFICPPQML
                     ETARVRAFRDWVFEEVGRFKQLFERACEAGPAASAGFAADALRVTP"
     gene            2133..2459
                     /locus_tag="VHV99_27175"
     CDS             2133..2459
                     /locus_tag="VHV99_27175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015001499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2917 domain-containing protein"
                     /protein_id="HEX3639100.1"
                     /translation="MREISSSIAFEIRSGETVPLKVARSTKLVVRGGAVWVTRSDDTE
                     DYWLQPGHTLRLRRGERLWLSGEGNTHAKVAFSVPTRCDERALNWFARVGERLAARMR
                     GGWRTV"
BASE COUNT          474 a          768 c          831 g          434 t
ORIGIN      
        1 ctggacaaga tcggcatcgt caatccgttc gacaacgccg gctggcgcga gctgatcgag
       61 cgcgtggtcg ccgccaatga ggcggacgcc ggcctgcgcg ccgatcagga tgcgattgcc
      121 tacgttcaga tcacgcgggg cgtcgcgaag cgcggtcacg catttccggc cggcgtccag
      181 ccgaccgtgt tcgtgatggt cacgccggtg aacctgccgg acgcggcaca acgcgcgcag
      241 ggcgtgcggt gtgtcaccgc tgaagaccgt cgctggctca attgcgacat caaatcggtg
      301 tcgctgctcg gtaatgtgct gatggcgcaa tacgcggccg aaaacgatgc gttcgagacg
      361 atccagtttc gcgacggcat gctgaccgaa ggttcatcgt cgaacatctg gatggcgaag
      421 gacggcgtgt tgtacgcgcc gccccgcagc cacaagattc tcgaagggat tcgctacggc
      481 ctgatcgaag aactgacgag cgaatgtggc atccgtttcg aagcgcgcga gatcgccgaa
      541 gccgaactgc gcaccgccga cgaggttctg gtcagttccg ccaccaagga agtgctgccg
      601 gtcacgcaga tagatggcca gccggttggc gacggcaagc cgggcgcggt gtttgcggcg
      661 ttgtacgcgg cttatcagcg tgccaaggcc aaggaagcgc aagaggcgca gcaaggagtg
      721 gaaagtgaat cccgcgaaag aatcattgct tgagtttccg tgcgactttc ccatcaaaat
      781 catgggcaag tcgcatcccg agttcgccga gacgattgtc acggtggttc ggcagttcga
      841 taacgaagtc gaccccgctc gcgtggaaac gcggccgtcc agcggtggca attacaccgg
      901 tttgacggtc acggtgcgcg ccacgagcca ggcgcagctc gacgatatct accgcgcgct
      961 caccggccat ccaatggtga aggtggtgct ttgacgcact gaccgggcag gcaccgacct
     1021 cgaccttggc gctcgctttg ccggccgtgt gaatgatgtc tcgtatcaag gcgtgacgcg
     1081 caatgcgtcc gccgcgaagc ccgcgcttgc cgcgggtcct gcctcgcaag cccgctcgaa
     1141 aagctgcttg aatcgcccca cttcctcgaa tacccaatcg cggaacgccc tgacccgcgc
     1201 ggtttcgagc atctgcggcg ggcaaatgaa gtagtactgc cacgggctcg gcccatccac
     1261 gtcgaatagc cgcaccaggc ggcccgctgc gatctcgtgc atcgccagcg aacggcgcgt
     1321 gagcgcgatc ccctggccgt cgatggccgc ctgcagcaga ttcgacgaat cctgatacag
     1381 cacgccgcgc tttggctccg gccagtcggc gaggccggcg gcgtcgaacc atgggcgcca
     1441 gagctcgtcg tccgagcgca gcagcggggc tttggccagc tcggctgggg tttgcggcag
     1501 cttgccaccg ttgaaatccg gcgcgcaagc cggaaagaaa atctcctcca gcagcaactc
     1561 cgcgtgcagc cccgaatatt tgccgtaacc gaagcggatc gccacgtcga cgtcgtcgcg
     1621 tgcgaagtcc gttagcgcgt tggtggacag caattcgagg tcccattgtg gatgcgcctc
     1681 gataaagctg ccgatacgtg gcgtcaccca acgcgccgcg aacgatgtca gcatggaaac
     1741 caccagccgc cgctcgcggt cgccggcgcg gacttcgcgc gtggcatcgg cgagtgccat
     1801 gagcgcggtg cgcacgcgcg tggcatagcg gcgcccggtg tccgtgagcc gtacgcgctt
     1861 gccgtcgcgg gcgaacagcg tgatgcccaa ctccgcttcc agcgcgcgaa tctgatggct
     1921 gacggcgccg tgcgtgacga acagttcgtc tgcggcgcgc gagaagctct cgtggcgggc
     1981 cgcggcttcg aacgccttga tcgcattcaa tgggggaagt tgccggaggt ccatcgcaat
     2041 atttctcaca tagaggtgaa aatagatcgt tttgcgtaaa cccttgttac gcctaggatt
     2101 gtagtcatga aacgattttt ggcgagggca tcatgcgaga aatttcctct agcatcgcgt
     2161 ttgaaatccg cagtggcgaa acagttcccc tgaaggtggc gcgcagcacc aagctggtcg
     2221 ttcgtggcgg ggcggtgtgg gtcacgcgca gcgacgatac ggaagactac tggctgcaac
     2281 ccggccacac actgcgactg cggcgcggcg aacggctgtg gctgagcggc gagggcaaca
     2341 cgcatgcaaa agtggcgttt tccgtgccga cgcgctgcga cgaacgtgcg ctgaactggt
     2401 tcgcgcgggt cggcgaacgg ctggcggccc ggatgcgtgg aggctggcgg acggtgtgaa
     2461 tgcagcaggc gccgccgtac cgcgaacgag cgcagttgca acgcgca
//
LOCUS       DATASH010001026         6653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5318763,
            whole genome shotgun sequence.
ACCESSION   DATASH010001026 DATASH010000000
VERSION     DATASH010001026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6653
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5318763"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            411..1595
                     /gene="pbpG"
                     /locus_tag="VHV99_27180"
     CDS             411..1595
                     /gene="pbpG"
                     /locus_tag="VHV99_27180"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HEX3639101.1"
                     /translation="MKTDMLSSLKMIHGAARSTALSVAATLVITAAFATPASSFAATP
                     DAPAKTSKHAKAAKKPAAATADKVSSKKPGKSAAVKTVAAASDDAPRAGVKRKRVTYT
                     SNGRHHSVVRRVAYEPRSPSVGQAFGLHETPDALMLRSSVAYVIDQNSGETLFDKNSR
                     AVVPIASITKLMTSMVVLDSKEPMTDQIEVTDEDRDYEKNTGSRLSVGSVLSREDMLH
                     IALMASENRAAAALSRYFPGGRPAFLAAMNAKAKQLGMTDTHFENPTGLTSQNVSSAR
                     DLVKMVNAAYQYPLIRKFSTDHSYEVYTGKRSLAYNSTNALVRNPTWDIGLQKTGFIN
                     EAGECLVMQATIHGRPMIIVLLDSSGKYSRFADATRLRTWLDNGGDQTRITSADAGGP
                     GT"
     gene            complement(1706..2512)
                     /locus_tag="VHV99_27185"
     CDS             complement(1706..2512)
                     /locus_tag="VHV99_27185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008924002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX3639102.1"
                     /translation="MSDTNPDPKTSIQVIERMMRLLDALAAHSDPVSLKELAIRTELH
                     PSTAHRILNDMVMCRLVDRSDPGTYRLGMRLLELGNLVKARLSVRDAALTPMRELHRQ
                     TGQTVNLSVRQGDEIVYIERAYSERSGMQVVRAIGGRAPLHLTSVGKLFLAADESTRV
                     RAYATRTGLSGHTQNSITDLTKLERELSHVRQQACARDNEELELGVRCIAAGIYDDTG
                     KLVAGLSLSAPADRLQDSWLGQVSQTALLISESLGYRPQTPQEHSHPQHV"
     gene            2693..3415
                     /locus_tag="VHV99_27190"
     CDS             2693..3415
                     /locus_tag="VHV99_27190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012400858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX3639103.1"
                     /translation="MRVGLFVTCLIDLMRPEIGFSVIKLIEGAGFEVVVPPAQTCCGQ
                     PAYNSGERRIARDLAEKTLREFEQFDYVVVPSGSCGGMIRAHYGDLFADDPELMNRFG
                     RLRAKVFELTDFLVNVAKVQLEPGEFAGQVTYHDSCSGLRELGVKAQPRALLAQVGVA
                     VTEMKDCEHCCGFGGTFAIKYGDISTAIVDEKCANIGASGAGAVVLGDLGCMLNIEGR
                     LRRTGNTTTRVLHIAQVLAGDA"
     gene            3577..4992
                     /locus_tag="VHV99_27195"
     CDS             3577..4992
                     /locus_tag="VHV99_27195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006408173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein B"
                     /protein_id="HEX3639104.1"
                     /translation="MQVQSMHFKARAGQKLADQRLQQNLTKLSTKFVSARAAAMTAID
                     FPATRAALKERRNRALENLDIWLETFEREATRRGVTVLFAETTQEAARLVGDIARRHE
                     VKKVIKTKSMVTEEMRLNEVLGQMGVQSIETDLGEYILQINDNEPPSHIIAPVVHKDK
                     DEIADLFAKTHNRPRLTEITDMTREAREILRPHFMTADMGVTGGNFVVAQTGSVVLVT
                     NEGNEGMCTVMPRVHVAVTGIEKVLPTLEDLATAMRLLPRSATGQATSNYFSVLTGPR
                     GADDQDGPEHMYVVLVDGGRTGLIGGDFQEMLRCIRCGACMNHCPVYQKVGGHAYGWV
                     YPGPMGAVLTPSYVGIDKALDLPQAATLCGECNSVCPVGIPLSDLLRKLREKQVERRL
                     RPWRERAGLAAWGFLALHPDAYALLTKLAVRVLERMGGRNRSIARLPLGGAGWTNTRD
                     MPAPVGRTFRELYAAQRSHLG"
     gene            complement(5218..5757)
                     /locus_tag="VHV99_27200"
     CDS             complement(5218..5757)
                     /locus_tag="VHV99_27200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639105.1"
                     /translation="MKSFAPLACLAVAALLGGCAVYPDGTPMYGNGYGGGYSGNDGYD
                     GYATGVPVVPQTNVYMGYSNYSGPGYYNGPGRYYGPGPGYGGYPDRGRSNNGNHGDNG
                     NWHGQSNGGSHGGGPPPQGAAGGPRGPSGSNAPRPAAGNPPPPPPQQAGNGGGRGNGN
                     NGAPVRGVPGRQSGQMTDH"
     gene            6026..6265
                     /locus_tag="VHV99_27205"
     CDS             6026..6265
                     /locus_tag="VHV99_27205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639106.1"
                     /translation="MKKTISVYWPVAIVVPLAAAAYLHVCGDAASRSSQAPLAAAQLT
                     AELARAVSYGMVDNATTLPAKPAHAVTGTPLGEAS"
     gene            6384..>6653
                     /locus_tag="VHV99_27210"
     CDS             6384..>6653
                     /locus_tag="VHV99_27210"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HEX3639107.1"
                     /translation="MKTVSICFVCLGNICRSPTAEGVMRHLVGEAKLAERILIDSAGT
                     GDWHIGQPPDERAQVAASRRGYELAALRGRQITATDFERFDLLIAM"
BASE COUNT         1241 a         1995 c         2111 g         1306 t
ORIGIN      
        1 ggatccgttg taaaccataa agtgtcacgg aacgttaatc ttacatgccg gtcgaatgcg
       61 cgttggcggt ttccagtagg cgtttaacag ttgcggcgac gtccggcatg ctggaatgtc
      121 atcctggcaa gttcccacgc ccgtgccctg acagtatttc ccctgatcaa gattgtctcg
      181 aaactgccgt tatgctgaaa gaatcgtctc gattccggcg tatggcgaca atggcgaggc
      241 aaaccacgct ggcgatcgtt ttcttcgatc gaagcgcggc acttatttgt gtttttgcga
      301 tcggagctta taagccggtt taaggagcgc gtataagtgc ttcaaattgc taatttttcg
      361 ttgttttact acacttgcgg aaacctctcg ccgctcccta ccgaacaccc atgaaaaccg
      421 acatgctttc gtcgctaaaa atgatccacg gcgcggcccg cagcaccgct ctgtcggtgg
      481 ccgccacgtt ggtcatcacg gcggcgttcg ccacacccgc cagcagtttt gccgccacgc
      541 ccgacgcacc tgcaaaaacc tccaaacacg cgaaagcagc caaaaagccc gccgcggcca
      601 ccgccgacaa agtgtcgagc aagaagcctg gcaaaagcgc ggctgtgaag acggttgccg
      661 ccgcgagcga cgacgcgccg cgcgcgggcg tcaagcgcaa gcgcgtgacg tacacctcga
      721 acggtcgcca tcactcggtg gtacgccgcg tcgcgtatga accgcgttcg cctagcgtcg
      781 gccaggcgtt tggtctgcac gaaacgccgg atgcgctgat gctgcgttca agcgttgcgt
      841 acgtgatcga tcagaactca ggcgaaacgc tgttcgacaa gaattcgcgc gccgtggtgc
      901 cgatcgcctc gatcaccaag ctgatgacct cgatggtcgt gctcgattcg aaagaaccga
      961 tgaccgacca gatcgaagtc acggacgaag accgcgatta cgagaaaaac accggctcgc
     1021 gtctgtcggt gggctccgta ctctcgcgtg aagacatgct gcacatcgcg ctgatggcct
     1081 cggagaaccg cgccgccgcg gcgctgtcgc gttatttccc gggcggccgc ccggcgttcc
     1141 tcgcggcaat gaacgcgaag gccaagcaac tcggcatgac cgatacgcac ttcgaaaacc
     1201 ccacgggttt gacgagccag aacgtgtcga gcgcgcgcga cctcgtgaag atggtcaacg
     1261 cggcgtatca atatccgctg atccgcaagt tctcgaccga tcacagctat gaggtgtaca
     1321 ccggcaagcg ttcgctggcg tacaacagta cgaatgcgtt ggtgcgcaac ccgacgtggg
     1381 atatcggtct gcaaaagacc ggctttatca acgaggcggg cgagtgtctc gtgatgcagg
     1441 cgaccatcca cggccgtccg atgatcatcg tgctgctcga ttcgtcgggt aaatattcgc
     1501 gctttgcgga cgcgacgcgt ctgcgtacgt ggctcgataa cggtggcgat caaacgcgca
     1561 tcaccagtgc ggatgccggc ggtccgggca cctgaagttg cctgacgtgc ggcagtctgc
     1621 agcggccggt tttcggaccg gccagaaaaa aagcctcgca aaatgcgagg cttttttatt
     1681 gagccgcatg aggacagcca tcagctcaca cgtgctgcgg gtgcgaatgc tcctgcggtg
     1741 tctgcgggcg gtatccgagc gactcggaaa tcagtagcgc cgtttggctg acctgcccca
     1801 gccacgagtc ctgcaagcga tccgccgggg cggacagcga caggccggcg accagcttgc
     1861 cggtgtcgtc gtaaatcccg gctgcgatac agcgcacgcc cagttcgagt tcttcgttgt
     1921 cgcgcgcgca ggcctgttgc cgcacatgcg atagctcccg ttcgagtttg gtcagatcgg
     1981 tgatgctgtt ctgcgtgtgc ccggacagac cggtgcgcgt ggcgtaggcc cgcacgcggg
     2041 tcgattcgtc cgcggcgaga aagagcttgc ctaccgaggt cagatgcagc ggcgcccgcc
     2101 cgccaattgc tcgcaccacc tgcatgccgg agcgttctga ataggcgcgc tcgatataga
     2161 cgatctcgtc gccctgacgc accgacaggt tcacagtctg cccggtttgg cggtgcaatt
     2221 cgcgcatcgg ggtgagcgcc gcgtcgcgta ccgacagccg cgccttcacc agattgccca
     2281 gttcgagcag gcgcatgccg agacggtaag tgccgggatc cgaccggtcg accagccggc
     2341 acatcaccat atcgttcagg atgcgatgcg cggtggacgg gtgcagctcc gtgcggatgg
     2401 cgagttcttt caggctgacc gggtcgctat gcgcagcgag ggcgtcaagc aagcgcatca
     2461 tgcgttcgat cacctggatc gaagttttgg gatccgggtt cgtatcgctc atggggagga
     2521 atcggttgac gcgtcaaaca gcggaaattg attgtatccc gtattgtgaa aagaaggaaa
     2581 gcggtgggaa cgcctattcc aatttagcgt ggcattcgtc ctatgccttc cggacgttgg
     2641 tattgcgcgg cgaacagcgg ataatcaggg acgttttccc gaaggagggc tcatgcgagt
     2701 cggattgttc gtcacctgcc tgatcgacct gatgcgtccg gagatcggtt tttcggtcat
     2761 caagctgatc gaaggcgccg gtttcgaggt ggtggtgcca cccgcgcaga cctgttgcgg
     2821 gcaacccgcg tacaactcgg gcgagcggcg catcgcgcgc gacctggctg agaaaacctt
     2881 gcgcgagttc gaacagttcg actacgtcgt ggtgccgtcc ggctcgtgcg gcggcatgat
     2941 ccgcgcgcat tacggcgacc tgtttgcaga cgatccggag ttgatgaacc gcttcggccg
     3001 attgcgggcc aaggtgttcg agttgaccga tttcctcgtc aatgtggcca aggtgcaatt
     3061 ggagcccggg gagttcgccg gccaggtgac ctaccacgat tcctgttcgg ggctgcgcga
     3121 acttggcgtc aaggcgcagc cgcgcgcgtt gctcgcgcag gtgggcgtgg cggtgaccga
     3181 aatgaaggat tgcgagcatt gctgcggctt tggcggcact ttcgcgatca agtacggcga
     3241 tatctccacg gcgatcgtcg acgagaaatg cgcgaacatt ggcgcgagcg gcgccggcgc
     3301 ggtggtgctc ggcgacctcg gctgcatgct caatatcgaa ggccgcttgc ggcgcaccgg
     3361 caataccacc acccgcgtgc tacacatcgc tcaggtgctg gccggcgacg cttgaaaatc
     3421 atcgcggcgc cacaaccggc gcacgtggtt cggtgcgttt catttaacgc agatcgcgcg
     3481 cgcttttttc actcgcgaat ttcattgggg ggcttcaccc aggaatttcg cccgcgaatt
     3541 catccgccag ttcatccgat taagctcaag gccgccatgc aagtccaatc gatgcatttc
     3601 aaggcccgcg cgggccagaa actcgccgac cagcgtctgc agcagaacct caccaagctg
     3661 tcgaccaagt tcgtgtcggc ccgcgccgca gccatgaccg cgatcgattt tcccgccacc
     3721 cgcgccgcgc tgaaggagcg ccgcaatcgc gcgctggaaa atctcgacat ctggctcgag
     3781 accttcgaac gggaagcgac ccggcgcggc gtcacggtcc tgtttgccga gacgacgcag
     3841 gaggccgcac gcctcgtcgg cgatatcgcg cgccggcacg aggtgaagaa ggtgatcaag
     3901 accaagtcga tggttaccga ggaaatgcgc ctgaacgaag tgctcggaca aatgggcgtg
     3961 caatcgatcg aaaccgatct gggcgaatac attctgcaga tcaacgacaa cgagccgccg
     4021 agccacatca tcgcgcccgt cgtccacaaa gataaggacg agatcgccga tcttttcgcg
     4081 aaaactcaca accggccgcg tttgaccgaa atcaccgaca tgacccgcga agcgcgcgag
     4141 atcctgcgtc cgcacttcat gacggcggat atgggcgtga ccggcggcaa cttcgtggtg
     4201 gcgcagaccg gttcggtcgt gctggtcacg aacgagggca atgagggcat gtgcacggtg
     4261 atgccacgcg tgcatgtggc tgtaaccggt atcgagaaag tgttgcccac gctggaagat
     4321 cttgcgacgg caatgcgtct gttgccgcgt tcggcaaccg gccaggccac gtcgaactat
     4381 ttttccgtgc tgaccggacc acgcggtgcg gacgatcagg acggtcccga gcatatgtat
     4441 gtagtgctgg tcgacggggg gcgcacgggg ctgattggcg gcgacttcca ggagatgctg
     4501 cgctgtatcc gttgtggcgc ctgcatgaac cattgcccgg tgtatcagaa ggtgggcggt
     4561 catgcgtacg gctgggttta tcctggcccg atgggggcgg tgctgacgcc gagctacgtc
     4621 gggatcgaca aggcgctgga tttacctcag gcggcgacgt tgtgcggcga gtgcaacagc
     4681 gtatgtccgg tgggaattcc gctgtccgat ctgctgcgca aattgcgcga aaagcaggtg
     4741 gagcggcggc tgcggccatg gcgcgagcgg gcagggctcg cggcgtgggg ttttctcgcg
     4801 ttgcacccgg acgcttatgc gttattgacc aagctggccg ttcgcgtgct ggaacggatg
     4861 ggcgggcgaa accggtcgat cgccagattg ccgctcggcg gcgcgggctg gactaatacg
     4921 cgggatatgc ccgctccggt cggtcggaca ttcagggagt tgtacgcggc gcagcgcagc
     4981 catctcggct gagttggcct tatcgggcgg agcgcgccgt cgtatttttc gagtttcgca
     5041 gcagtaagcg cgagccggtg attccggcgc agcgctgttt aagttaacgg ccgcggcgct
     5101 tggcgcgctc ccccggtgat tgtcaggccg ggttatcagg cagtagtcgc agagaccgga
     5161 gcgcagacgc gtgcgctgat aagtctgcgt gtcgcgcaac tacgcgacac gcccgtctca
     5221 gtggtccgtc atttgaccag actgtcggcc aggcacaccc cgcaccggag ccccgttgtt
     5281 gccgttgcca cgaccgcctc cgttgcctgc ctgttgcggc ggcggcggcg gtgggttgcc
     5341 agccgccggt cgaggagcat tcgatccgct cgggccacgc ggcccgcctg cggcgccttg
     5401 aggcggcggg ccgccgccgt gcgagccgcc gttcgactga ccgtgccagt tgccgttatc
     5461 gccgtggttg ccgttatttg accggcctcg gtcgggatag ccgccatagc cgggtcccgg
     5521 tccgtaatag cggcccggcc cgttgtagta ccccgggccc gaatagttgc tatagcccat
     5581 gtagacgttc gtttgcggca cgaccggcac gccggtggca tagccgtcgt aaccgtcatt
     5641 gccgctgtaa ccgccgccgt agccattgcc gtacatcggc gtgccgtccg gataaaccgc
     5701 gcagccgccc agcaaggcgg cgactgcgag gcaggcgagt ggtgcaaagg atttcatgtt
     5761 atcgaccagt gcgagatttc gtttctgtaa gaccgctatt atcgccatcc gtcgcaagcg
     5821 ctttgtatgt gtttgtaagg atttcttttt cgtgccatga cgcgcgtcag tgccgtgcac
     5881 cgcaccatag ccagttgact gttccactta gagcaacgct ctatctcgcg ctagtgggtt
     5941 ttccccgatt gtgaatttct ataggattcg ggatgggcat agtgagttgc aaactcaccg
     6001 gtccttaatc ggaagaaacg acgtcatgaa aaagacaata tcggtgtact ggcctgttgc
     6061 catcgttgta ccgcttgccg cagcagctta tctgcacgtc tgcggcgatg ccgcttcgcg
     6121 ttcgtcgcaa gccccgctcg cggctgccca actgactgcg gaactggcgc gtgccgtctc
     6181 ctacggcatg gtcgacaacg cgacgacgtt gcccgctaaa cccgcgcacg ctgtaacggg
     6241 cacgccgctg ggcgaagcat cctgagcgca gcgagatcgg gcagtgcgtc gcgtgccccg
     6301 ggcgcaatct ggctcaactt tgtataatcg cggcaccgtt accaactgcc gatccacgcg
     6361 cgttttgcgc ccaaaagcct ctgatgaaaa ccgtgtccat ctgctttgtc tgcctgggga
     6421 acatttgccg ttcgccaacc gcggagggcg tgatgcgcca tctggtcggg gaggccaagc
     6481 tagcggagcg aatcctgatc gattccgccg gcacgggcga ctggcacatt gggcagccgc
     6541 ccgacgagcg cgcgcaggtt gcggccagtc ggcgaggcta cgagctagcg gccttgcgcg
     6601 ggcgccagat tacggcaacc gatttcgagc gcttcgatct gctcattgcg atg
//
LOCUS       DATASH010001027         8792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_967940,
            whole genome shotgun sequence.
ACCESSION   DATASH010001027 DATASH010000000
VERSION     DATASH010001027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 8792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8792
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_967940"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..519)
                     /locus_tag="VHV99_27215"
     CDS             complement(55..519)
                     /locus_tag="VHV99_27215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007585642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0048029 - monosaccharide binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU domain-containing protein"
                     /protein_id="HEX3639108.1"
                     /translation="MLKNLDPLLNADVLHALRAMGHGDELVVCDANFPGASVAQQTVL
                     GKLLRLDGVNAPRAIRAILSVMPLDTFIEHPALRMEVVGEPNTIPAVQREAQIEINAA
                     EGREVPFASVERFAFYERARKAYCVIATGEARGYGCFVFTKGVLLAPDAPQQ"
     gene            complement(683..943)
                     /locus_tag="VHV99_27220"
     CDS             complement(683..943)
                     /locus_tag="VHV99_27220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639109.1"
                     /translation="MHESAAATAPGIGLTVARLAGAVDALSSAIAGFALLAGARAFTF
                     TALLAWREPVLGGAGLVLLALVGWLRWQCRQNRLGASCAKAD"
     gene            complement(971..1336)
                     /locus_tag="VHV99_27225"
     CDS             complement(971..1336)
                     /locus_tag="VHV99_27225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX3639110.1"
                     /translation="MNAHTDLIDRYFDAWNETDAARRRELIAATWAANADYRDPLLAG
                     AGHDGIDAMIRAVHERFPHHTFHRTTEVDGFANRLRFSWALTTPAGEAIVKGSDFGVL
                     DAHGRLQAVTGFLDQVPAA"
     gene            1471..1998
                     /locus_tag="VHV99_27230"
     CDS             1471..1998
                     /locus_tag="VHV99_27230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX3639111.1"
                     /translation="MIHALLAAGLAIRPNRHGRRQPLISLGKWEPRSGDGRHVMNSTG
                     ADTNRFLNMVFTLARSYPTGYTHQQIGSPPVYTVNRTEEFDSWLAGLADLRARAKILV
                     RVRRAERGHFGDVKLLEDGVSEMRIDCGPGYRVYFAREGHSVYLLLCGGDKSSQPADI
                     KHAKSMWAAIRKEQS"
     gene            1995..2297
                     /locus_tag="VHV99_27235"
     CDS             1995..2297
                     /locus_tag="VHV99_27235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antidote protein"
                     /protein_id="HEX3639112.1"
                     /translation="MSKVKTARFDASHYLDSEEMIAEYLNAALEEGDADLLLAAIADI
                     AKARGIAKVAADAGLGRESLYKTLAPGSKPRMDTVFKLLRALGVKLNAVPEGVAHA"
     gene            2426..3325
                     /locus_tag="VHV99_27240"
     CDS             2426..3325
                     /locus_tag="VHV99_27240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX3639113.1"
                     /translation="MDRSRALAIIGNMNTLSLAQTIPSNVPTASRTVGDLLREWRQRR
                     RMSQLLLAAEADISTRHLSFVESGRAVPSREMVMHLAERLDVPLRARNALLVAAGYAP
                     LFRERPLTDPQLAAAREAVELVLKGHEPYPALAIDRHWTIVAANNALAPLLAGASPEL
                     LKPPVNAMRLSLHPEGIAASIVNWHAWRAHALSRLQRQIDVSGDDTLSALRDELTTYP
                     APPGVEVADHDDTATNQVAVPLRLRTPIGELSFFSTTTVFGTPVDVTLSELAIEAFFP
                     ADQQTAAALREFAENQRAEGQRA"
     gene            3420..3950
                     /locus_tag="VHV99_27245"
     CDS             3420..3950
                     /locus_tag="VHV99_27245"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX3639114.1"
                     /translation="MVNNVPLVRPLGPADRDAYFQLRLRGLKAHPESFGQSYEEAVAK
                     GAARHDAMLQGARAAEGDFLLGAYASANAPLIGVVGLLRSQSDKERHKASVVGMYVAP
                     EAAGRGVGRALLNELLARAARIEGLRQIQLMVGSRNEAARKLYESLGFRKYGCEIGAL
                     NVNGVFHDADLMARFM"
     gene            4093..5316
                     /locus_tag="VHV99_27250"
     CDS             4093..5316
                     /locus_tag="VHV99_27250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HEX3639115.1"
                     /translation="MSDLSTSLPRIPRRFDINPKPLGAALVLIALGAVYLAQTVSGRQ
                     AALYIVGALLGMSLYHAAFGFTSAWRVFIADRRGAGLRAQMLMLALGVLLFFPALAAG
                     TLFGQPVVGLVAPAGTSVLVGAFMFGIGMQLGGGCASGTLYTVGGGSTRMIVTLVAFV
                     IGSVVATAHMPFWTALPSLKPLSMVTALGAGWAIVLNLVIFAAIAALTVWVERRRHGR
                     LINEPLREPHASPWLHGPWPLFVGAIALVLLNFATLALSGRPWGVTSAFALCGAKLFV
                     ALGIDVANWPYWAAHANAGALAAPVTHDVTTVMDIGIVLGAMMAAALAGRYAPVWKVP
                     ARSLIAAVVGGLMLGYGARLAYGCNIGAYFSGIVSGSLHGWLWLVAAFAGNVLGTRLR
                     PAFGLEVERVQASGC"
     gene            5837..6157
                     /gene="flhD"
                     /locus_tag="VHV99_27255"
     CDS             5837..6157
                     /gene="flhD"
                     /locus_tag="VHV99_27255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007585797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HEX3639116.1"
                     /translation="MDRSSETLDSIREINLSYIMLAQRMLREDKPVGMFRLGLSSELA
                     DLLAGLSLAQIVKLAASDQLLCFFRFNDHAMLSALTQTTKHAAVAPTHAAILLAGQPA
                     EQFA"
     gene            6172..6741
                     /gene="flhC"
                     /locus_tag="VHV99_27260"
     CDS             6172..6741
                     /gene="flhC"
                     /locus_tag="VHV99_27260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HEX3639117.1"
                     /translation="MLKRSLTEDAQEVFRAIALIELGARMQVLESELTLSRDRMIRLY
                     REVKGVSPPKGMLPFSADWYMTWLANIHASLFYNTYLFLKKEARCSHLDALTKGYRLY
                     LEHCQHSETDPVLDLTRAWTLVRFFDADILQLTKCCRCTGKFVAHKHDLQHNVVCGAC
                     QPPSRAGKTKKAAAARQEALEAAQIEQAA"
     gene            complement(6941..8269)
                     /locus_tag="VHV99_27265"
     CDS             complement(6941..8269)
                     /locus_tag="VHV99_27265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013342730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEX3639118.1"
                     /translation="MQFKLPTTATAPFCPSEVQGSVTVTQGAPFWKKILQFAGPGLLI
                     SIGYMDPGNWATDIEAGSRYGYSLLFVVMLSSLAAMALQCLSMRLGIATGRDLAELSR
                     LRYSPGVARFQWLLAELSIVACDLAEVLGGALAFHLLFKCSLTTGVLLTAFDTLIVLG
                     LKGKNFRDLEAIMLGLIATIGVGYIVELALVKPHWPSVAQGLIPSWQALSSREPMYLA
                     IGILGATVMPHNLYLHSSIVQTRAVKRDSASIKSAIGMSRIDTIVSLVLALLINMAIL
                     ILAAAAFHATGHNQVTEIEDAYKLLAPVVGTGFAAVLFAITLLASGQSSTFTGTVAGQ
                     VIMEGFLKLKIPCWQRRFITRVLALIPALIGVQMMGNGAVGKLLVASQVVLSLQLPFA
                     LYPLIRMTNDRSLMGEFANRLPTRLVAWSLFVVISVANLWLVVQTVGLVD"
     gene            complement(8330..>8792)
                     /locus_tag="VHV99_27270"
     CDS             complement(8330..>8792)
                     /locus_tag="VHV99_27270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004529865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX3639119.1"
                     /translation="IAQGALEEARAYVTQHGKPWIASGVDKATDDPYLIQRFGEMRLQ
                     AVSAEALATRAAHALEDAWQQGPALDAETRAHVALATSEAKIVAHRAALDVSEQLFDA
                     CGARATHAPLALDRFWRNARVHTLHDPLDYRVRDVGRYALSGTLPEVSLYT"
BASE COUNT         1706 a         2741 c         2677 g         1668 t
ORIGIN      
        1 atgatcgaga ctgtctatcg agaagctcat gaggttcgtc gccatggaga aggactattg
       61 ctgtggcgca tccggcgcga gcaagacgcc cttggtaaaa acgaagcagc cgtaaccgcg
      121 cgcttcaccg gttgcgatca cgcaataagc cttgcgcgcc cgctcataga aagcgaaacg
      181 ttcgaccgac gcaaacggga cctcacgccc ttccgccgca ttgatctcga tctgcgcctc
      241 gcgctgaaca gccggaatcg tattcggctc gcctaccact tccattcgca aggcagggtg
      301 ttcgatgaag gtatccagcg gcatcactga aagaatcgca cgaatcgcgc gcggtgcgtt
      361 cacaccgtcg agtcgcagca gcttgcccaa caccgtctgc tgcgcgacgg acgcgcccgg
      421 gaagttggcg tcgcaaacca ccagttcgtc gccatgtccc atcgcgcgta gcgcatgcag
      481 gacatcggca ttcagcagcg ggtccagatt tttcagcacg cgttgtctcc ttgaaatctt
      541 gaggtccttg aggtccttga ggtccgattc ggcggctagc cgtccgggtc cgaacggttt
      601 gtccttgtag cgctgttctt ccaggcattg agattgattg tagcaagacc gtagcgcacg
      661 gcccggtgcc gacacctgat cgtcaatccg cttttgcaca cgatgcgcca agccgattct
      721 gacgacactg ccaccgcagc catccgacca gcgcaagcag cacgagaccc gcgccaccca
      781 acaccggctc gcgccaggcc agcaacgcag tgaaggtgaa cgcgcgcgca ccggccagca
      841 gagcgaagcc ggcaatcgca ctggagagcg cgtcgacggc gcccgcgagg cgcgcgaccg
      901 tgaggccaat tccgggagcc gtggcggcag cggactcgtg catgatcgtc tccgtaaaaa
      961 tcgtggctgg tcaggcggca ggcacctggt cgaggaagcc ggtcaccgcc tgcaggcggc
     1021 cgtgtgcgtc gagcacgccg aagtcggatc ccttcacgat tgcctcgccg gccggcgtgg
     1081 tgagtgccca cgaaaagcgc agccggttcg cgaagccgtc gacctccgtc gtgcggtgaa
     1141 acgtgtgatg cggaaagcgt tcatgcaccg cacgaatcat cgcgtcgatg ccgtcatggc
     1201 ccgcgcctgc cagcagcgga tcgcgatagt cggcattggc ggcccatgtc gccgcgatca
     1261 gttcgcggcg gcgcgctgca tcggtttcat tccatgcgtc gaaataacgg tcgatcagat
     1321 cggtatgcgc attcatctca aactccttga ttggcatgac tgagcactac gctcggcaaa
     1381 cacgttgtat gcggcgggct cggagtgaag attggcgctt cgagcgcagc gcgtcgatta
     1441 cgtgggaggt aagcatccgg atgcaccggg gtgatccacg cccttttggc cgccggattg
     1501 gccatccggc cgaatcgtca cgggcggcgt cagccgctaa tctcattggg caaatgggag
     1561 ccgcgcagcg gtgacgggcg gcatgtgatg aactcaacgg gtgcggacac gaaccgcttt
     1621 ttaaatatgg tttttacgct tgcaagatcg tatccgacag gatacactca tcaacaaatc
     1681 ggttcaccgc ctgtgtacac ggtcaaccgc accgaggaat tcgacagttg gcttgccggc
     1741 ctcgcagatt taagagcgag ggcaaaaatc ctggtgcggg tccggcgtgc ggagcgaggt
     1801 catttcggcg acgtaaagct gctggaagac ggtgtgtccg agatgcgcat cgattgcggt
     1861 cccggctatc gggtctactt cgcgcgcgaa gggcactcgg tgtacctgct gctctgcggg
     1921 ggcgataaat cctcgcagcc agccgacatc aagcacgcca aatcaatgtg ggcagccatc
     1981 agaaaggaac agtcatgagc aaagtcaaaa ccgcacggtt cgacgcatcg cactacctcg
     2041 atagcgaaga gatgatcgct gaatatctca atgcagcgct ggaagaaggc gacgccgacc
     2101 tcctcctcgc cgcgatcgcc gatattgcaa aagcacgcgg catcgcgaag gttgcggccg
     2161 acgccgggct cgggcgtgaa agcctctata aaacactcgc gcccggctcc aaaccgcgca
     2221 tggatacggt gttcaagttg ctgcgcgcgc taggcgtcaa gctcaacgcc gtgccggaag
     2281 gcgtggcgca cgcctgactc gcgcctgccg cgttgtcgcc tggggcaacg ccccaaatgt
     2341 gccgcgtatt agtggatgtc ctacgttttc tcggatattc ggggtctgcc cgggtcttcc
     2401 cgggtcttcc attacctgtc aagtaatgga tcgctcacgc gctttggcta tcatcggcaa
     2461 catgaacaca ctctctctcg cgcaaaccat cccgtcgaac gtgccgacgg caagccgcac
     2521 ggtcggcgat ctgctgcgtg aatggcggca acggcgaaga atgagtcagt tgctgctcgc
     2581 cgctgaagcc gatatttcca cgcggcacct gagcttcgtc gaatcagggc gtgcggtgcc
     2641 cagccgggaa atggtcatgc acctcgccga gcggctcgac gtgccactgc gggcacgcaa
     2701 cgcactgctg gttgcggcgg gctatgcgcc gctgttccgc gagcgtcctt tgacagaccc
     2761 gcaactggcc gccgcgcgcg aggcagtgga gttggtgctc aaaggtcacg agccatatcc
     2821 ggcactcgcg atcgaccgcc actggaccat cgttgccgcg aacaatgcgc ttgcgccgct
     2881 gcttgcaggc gccagcccgg agctgctgaa accgccggtc aatgcgatgc ggctgagctt
     2941 gcatccggaa ggcatcgcag cgtcgatcgt caactggcat gcctggcgcg cgcacgcgct
     3001 gtcccggctc cagcggcaaa tcgacgtgag cggtgacgat acgttgagcg cactgcgcga
     3061 tgagctaacc acgtatcccg ccccacccgg cgtggaagtg gccgaccacg acgacaccgc
     3121 gaccaatcag gtcgccgtgc cgctgcggct gcgcacgccg atcggtgaac tgtcgttctt
     3181 cagtacgacc acggtattcg gaacgccggt ggatgtaaca ctgtcggaac ttgccattga
     3241 agcgttcttc cccgccgacc agcaaacggc ggcggcattg cgcgagtttg ccgaaaacca
     3301 gcgcgccgaa ggccaacggg catgaagccg tgaagccacg gcaggactgc agaatcccga
     3361 gcaacgtgaa cactgggtgt taccgcttgc agcatcattc aaaccacacg gagacgcatt
     3421 tggtcaacaa cgtcccactg gtgcgcccgc tcggaccggc cgatcgtgat gcctacttcc
     3481 agcttcgcct gcgcggactg aaggcacatc cggagtcatt cggtcaaagc tacgaggaag
     3541 cggtagccaa gggcgcagcg cggcatgatg cgatgttgca gggcgcacgc gctgccgaag
     3601 gcgacttttt gctcggtgca tacgcatcag ccaatgcgcc gttgatcggc gtggtcggac
     3661 tgctgcgcag ccagagcgac aaggaacggc acaaggcctc cgtcgtcggc atgtatgtcg
     3721 cgccggaggc tgcgggccgc ggagtcgggc gcgcattgct taatgagttg ctggcgcgag
     3781 cggcgcgaat agaaggcttg cggcagatcc agttgatggt gggcagccgg aacgaggcag
     3841 cacgcaagct ctacgagtcg cttggttttc gcaaatatgg ttgcgagatc ggcgcgttga
     3901 acgtcaacgg ggtattccac gatgccgatc tgatggcgcg cttcatgtaa cagccaacgc
     3961 ggagacaacc gccctcctca agcgcgcgat ccgtgctatc gttttttgtt acctttccac
     4021 cgcaccagcc ccgaagcggc tcatccgccg ccctcgggct atcttcgatt tgccgctcgt
     4081 cagtcaaata ccatgtccga tctctccact tcgcttccgc gcataccccg ccgtttcgac
     4141 atcaatccga aaccgctagg cgccgcgctc gttctaatcg cgctgggcgc cgtctatctg
     4201 gcgcaaaccg tgagcggccg ccaggcggcg ctctacattg ttggcgcact gctgggcatg
     4261 tcgctttatc acgcggcgtt cggctttacc tccgcatggc gggtctttat cgcagaccgc
     4321 cgcggcgccg gtctgcgcgc gcaaatgctg atgctggcgc tcggcgtcct gctgttcttt
     4381 ccggcgctcg ccgcaggcac gctgttcggc cagccggtgg tcggcctggt cgcgccggca
     4441 ggcacgtccg tgctggtcgg cgccttcatg ttcggtatcg gcatgcagtt gggcggcggt
     4501 tgcgcgtccg gcacgctgta caccgtgggc ggcggcagca cccgcatgat cgtcacgctg
     4561 gtggcgttcg tcatcggatc ggttgtcgca accgcgcaca tgccgttctg gaccgcactg
     4621 ccgtctctca aaccgctttc gatggtcacc gcgctcggag cgggctgggc catcgtgctg
     4681 aatctggtga tattcgctgc gatcgcggcg ctcaccgtat gggtcgaacg gcgccgtcac
     4741 ggccggctga tcaacgaacc gctgcgcgag ccgcacgcgt caccatggct gcacggcccg
     4801 tggcccttgt tcgtcggcgc aatcgctctg gtcttgctca atttcgcgac cctggcgctg
     4861 tccggccgcc catggggggt gacgtcggcg ttcgcgctgt gtggcgccaa gctatttgtc
     4921 gcgttgggta tcgatgtggc caattggcca tactgggccg ctcatgccaa cgccggagcc
     4981 ctggccgcgc ctgttacaca tgacgtgacg acggtgatgg acatcggcat cgtcctcggc
     5041 gccatgatgg ccgccgcgct cgccggccgt tacgcaccgg tctggaaggt accggcccgc
     5101 tcgctgatcg cggccgtggt cggcggcctg atgctcggct acggggcccg cctggcttac
     5161 ggttgtaaca tcggcgccta cttcagcggc atcgtgtcag gcagcttgca cggctggttg
     5221 tggctggtcg ccgcgttcgc cggcaacgtg ctgggcaccc ggttgcgtcc cgcattcggc
     5281 ctcgaagttg aacgggtcca ggccagcggt tgctaggcgt tacaaatgcg gcctcatttg
     5341 taactggggc cgcacccatc tgttacacct ctgatgcgtc cccatagcaa cgtcaacgaa
     5401 cggtatttat tacttttaat tgaattcgct ttctatttcc tttccacgct cactcccgat
     5461 tttccggttt cctaccagac ccgcgccgtt cttggacaaa tcatccagac ccccatcaca
     5521 caagggtttc catggatcag acggaattct cggggcgcaa aaaacctgcg aagtgccacc
     5581 tattacaaga ccgtgaaaga actaccgata tttgatgtat ctttttgaga tatttttttg
     5641 agaagggatt gatttcttgt tttacccttc atacaattcg acccaagctg ttacgaaatg
     5701 taacgcgatg tatcaaaaag tcgatgtctg tatcaggtgg tcacatgtag ccggaggttc
     5761 tcatttccgg cgaggcacaa aagacataac ggcaaaaaat gccgacatag taattcgggg
     5821 cttcggaaac aaaaaaatgg accgtagcag cgagacgctg gattcaatcc gcgagattaa
     5881 cttgtcttac atcatgctcg cgcaacgcat gttgcgcgag gacaaaccag tcggcatgtt
     5941 ccggctggga ctgtcgtcgg aactggctga tttgcttgcc ggtctgtcgc tcgcgcagat
     6001 cgtcaagctg gccgcttccg atcagctttt atgcttcttc cgcttcaacg accacgcgat
     6061 gttgtcggcg ttgacgcaaa cgacgaagca cgcagccgtt gcaccgactc acgcggcgat
     6121 cctgcttgca ggccagcccg ctgagcagtt tgcttaatcg aggtaaacgc gatgctcaag
     6181 cgtagcctga ccgaagacgc acaagaagtc tttcgcgcca tcgcgctgat tgaactcggc
     6241 gcgcgtatgc aagtgctcga aagcgaattg acgctttcgc gcgaccgcat gatccgcctg
     6301 taccgtgagg tgaaaggcgt atcgccgccc aagggcatgc tgccgttctc ggcggactgg
     6361 tacatgacgt ggctggcgaa catccacgcg tcgttgttct acaacacgta tctgtttctg
     6421 aagaaggaag cgcgctgctc gcacctggac gcgctgacca aagggtatcg gctgtatctg
     6481 gaacattgcc agcacagcga aaccgatccg gtgctggatc tgacgcgtgc atggacgctg
     6541 gtgcgtttct tcgacgctga tatcctgcaa ctgaccaaat gctgccgttg caccggcaag
     6601 ttcgtcgcgc acaagcacga tctgcaacac aacgtggtgt gcggcgcttg tcagcccccg
     6661 tcgcgcgccg gcaagaccaa gaaggccgcc gccgctcgcc aggaagcgct cgaagccgcg
     6721 cagatcgaac aagccgccta atcgagtcgt atcgatgagg gccggtccag cctgattcga
     6781 accgaagcaa cccggaagaa ggtccgctag cgcgcggacc tttttacatt gtgtttgccg
     6841 ccgtcagaat cgcgcccgcg atcttgcccg cgatcccgga gcggagtgag cccgttcctc
     6901 aacccggcat cgggcgacag gcgcctgtcc ctacgcccag tcaatccacc agtcccaccg
     6961 tctgcaccac cagccacagg ttcgccacac tgatcaccac gaacagcgac caggcgacga
     7021 gccgcgtcgg cagccggttg gcgaattcac ccatcagcga acggtcgttg gtcatgcgga
     7081 tcagcggata aagcgcgaac ggcagttgca ggctcagcac gacctggctt gcgaccagca
     7141 atttgccgac cgcgccgttg cccatcatct gcacgccgat cagcgccgga atcaaagcga
     7201 gtacccgtgt gatgaagcgc cgctgccagc acggaatttt cagcttaaga aagccttcca
     7261 tgatgacctg ccctgccacc gtgccggtga aggtcgaact ttgccccgac gccagcagcg
     7321 tgatcgcgaa cagcaccgcc gcgaaccccg tgccgacgac cggcgccagc agcttgtacg
     7381 cgtcttcgat ctcggtgacc tgattgtggc cggtggcatg gaacgcggcg gccgcgagaa
     7441 tcaggatcgc catgttgatc agcaacgcga gcaccagcga gacgatcgta tcgatgcgcg
     7501 acatgccgat cgccgacttg atgctggctg aatcgcgctt caccgcgcgc gtctgcacga
     7561 tcgacgaatg cagatagagg ttgtgcggca tcacggtggc gccgagaatg ccgatcgcca
     7621 gatacatcgg ctcgcggctg ctgagtgcct gccacgaagg aatcagtcct tgtgcaacgg
     7681 acggccaatg cggcttcacc agcgccagtt cgacgatgta gccaacgccg attgtcgcga
     7741 tcaggccgag catgatcgct tcgaggtcgc ggaaattctt gcccttcagg ccgagcacga
     7801 tcagcgtgtc gaacgcggtc agcaacacgc cggtggtgag cgagcacttg aagagcagat
     7861 ggaaggccag cgcgccgccg agcacttcgg ccagatcgca ggccacgatc gacagctcgg
     7921 caagcagcca ctgaaagcgc gcgacacccg gtgaataacg cagccgcgac aattccgcga
     7981 gatcccgccc ggtggcgatg ccaagacgca tgctcaggca ttgcagcgcc atcgccgcaa
     8041 ggctcgacag catgacgacg aacagcaggc tgtagccgta gcgcgaaccc gcttcgatat
     8101 cggtggccca gttgccgggg tccatgtagc cgatcgaaat cagcagaccg ggtccggcga
     8161 attgaagaat ttttttccag aatggtgcgc cctgcgtgac ggtgaccgag ccttgcacct
     8221 cggatgggca aaacggcgct gttgcggtag tcggtaattt gaactgcaat ccgatgatct
     8281 cttgaagggg acaaaccgca gcgctccgtg ccggacgccg cgaaccgcct caagtgtaca
     8341 aagaaacttc cggcaacgta ccgctcagcg cataacggcc gacgtcgcgc acgcgatagt
     8401 cgagcggatc gtgcagcgtg tgcacgcgcg cgttgcgcca gaagcggtcg agcgcgagcg
     8461 gcgcatgcgt ggcgcgcgcg ccgcaggcgt cgaagagttg ttcgctgacg tcgagcgcgg
     8521 cgcggtgcgc gacgattttc gcctccgagg tggcgagtgc gacatgtgca cgggtttcgg
     8581 cgtccaaagc cgggccctgt tgccacgcat cttcgagcgc gtgggccgca cgcgttgcca
     8641 gcgcttccgc actcaccgcc tgcaggcgca tttcgccgaa acgctgaatc aggtagggat
     8701 cgtcggtggc tttatcgaca ccggaagcga tccacggctt gccgtgctgc gtgacgtacg
     8761 cgcgtgcttc ttcaagcgcg ccctgggcga tg
//
LOCUS       DATASH010001028        11814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6503313,
            whole genome shotgun sequence.
ACCESSION   DATASH010001028 DATASH010000000
VERSION     DATASH010001028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 11814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11814
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6503313"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1075
                     /locus_tag="VHV99_27275"
     CDS             <1..1075
                     /locus_tag="VHV99_27275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HEX3639120.1"
                     /translation="AIKASTTGCDDIAFWLYSSGSTGKPKGTVHTHANLYWTAELYAK
                     PILGIVESDVVFSAAKLFFAYGLGNGLTFPLSVGATAVLMAERPTADAIFTRLVRHRP
                     TVFYGVPTLYASMLVSPNLPARADVAMRVCTSAGEALPREIGERFTAHFGCEILDGIG
                     STEMLHIFLSNRAGQVEYGTTGRPVPGYEVELRDEAGHAVPDGEVGDLYIKGPSAALM
                     YWSNREKSRATFLGEWIRSGDKYCRLANGCYVYAGRSDDMLKVSGQYVSPVEVEMVLV
                     QHDAVLEAAVVGVDHGGLVKTRAFVVLKREFAPSEILADELKAFVKERLAPHKYPRDI
                     LFVDDLPKTATGKIQRFKLREQS"
     gene            1084..1911
                     /locus_tag="VHV99_27280"
     CDS             1084..1911
                     /locus_tag="VHV99_27280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006052519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3639121.1"
                     /translation="MQNPATAISNSDFAELPATASHGPLRIEYRWLNEAAANAPIAVF
                     LHEGLGSVAMWRDWPQSLCEQLGMRGLVYSRPGYGLSTPREHDVKWPVDFMTAQARDI
                     LPALLDALDINMQERKRMWLIGHSDGGSIALLYAALHPEALAGAVTIAPHVFVENISV
                     ESIAQTKQLYETTDLRSKLSRYHADVDSAFYGWNDIWLNPAFREWRIVDELSAIRQPL
                     LAVQGHDDNYGTMAQIDTIGERVPHAQLVKLDACGHSPHRDAPVALNEAIAAFISRH"
     gene            2085..2408
                     /locus_tag="VHV99_27285"
     CDS             2085..2408
                     /locus_tag="VHV99_27285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639122.1"
                     /translation="MSAVSKPTAALLRKRLAGALIVMSLCGMLGACGNGDDPSPDTHA
                     ASSTDNGGTSQVLSANPSSAAPASPALPALTIQTPASPASSADTALSAAAATPLATPV
                     IHTVD"
     gene            2490..4601
                     /locus_tag="VHV99_27290"
     CDS             2490..4601
                     /locus_tag="VHV99_27290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004629 - phospholipase C activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase C, phosphocholine-specific"
                     /protein_id="HEX3639123.1"
                     /translation="MTSKNRRDFLRSAAHAAGSATALSMLPLGIRNALAIPANNKTGS
                     IRDVEHIIVLMQENRSFDHYFGTLKGVRGFGDTRAINLPNGKPVWYQPLAADAGYVLP
                     FRPTAPTLGLQFLQDLAHDWTTTHAAWNGGRYDQWVPSKGTTTMAYFTRDDIPFHYQL
                     ADAFTICDAYHCSLLGPTDPNRYYMWSGWVGNDGNGGGPVIDNSELGYGWSTYPEVLQ
                     NAGITWKIYQDIGTGLNASGSWGWTQNPYIGNFGDNSLLYFNQYRNAQPGNPLYDNAR
                     IGTNAANGDGFFDILKRDVQNNALPQVSWIVAPEAYSEHPNWPTNYGAWYIDQVLQVL
                     TSNPEVWSKTVLLINYDENDGFFDHVAPPFAPASSANGLSTVDTSNENYAGDSKTPAG
                     PYGLGPRVPMLVVSPWSKGGYVCSELFDHTSVIRFIEKRFGQQHNLGESNITPWRRAV
                     CGDLMSAFNFSNPNDAFPALPSTSGYVPPDQNRHPDYVPLPPLLQAVPKQEPGVRPAR
                     ALPYELFVRVQGEGSGNKLTMRFVNSGRAGAVFLVYAANGLDAPRTYTVEAGKQLQDS
                     LPLNADGTYDFTVHGPNGFLRRFAGKPVARNWWNGSDIARPDVAEGYDVANGNLQLRL
                     ANAGSARCQFTIVNAYDANNVIKHSVRGGDTEQLYLDLRNAHGWYDLAITVDTDPSFA
                     RRFAGHVETGESSMSDPALGS"
     gene            complement(4702..5082)
                     /locus_tag="VHV99_27295"
     CDS             complement(4702..5082)
                     /locus_tag="VHV99_27295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015600936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5594 family protein"
                     /protein_id="HEX3639124.1"
                     /translation="MRPESAARFDKQFAPRIAEALAACFATSVHTEVLPYGGHGHPTR
                     VRIHATPIEDLRHYPHPLNLFLTWDGDEIERLMGSAGAARFAGYLAALPRKLEAWRHV
                     RELDFLSHTQAEPAVLIGGLDFES"
     gene            complement(5203..5553)
                     /locus_tag="VHV99_27300"
     CDS             complement(5203..5553)
                     /locus_tag="VHV99_27300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEX3639125.1"
                     /translation="MCGIYINADPILYESRTRSLRIHGVITTVRLENLFWDVLHEIAA
                     RENMTTSQFAVKLYDELIALRGEMPTNFASFLRVCCLRYLSMRTEKATSVSDATPEPA
                     AVVNETRPRILKKV"
     gene            complement(5701..7287)
                     /locus_tag="VHV99_27305"
     CDS             complement(5701..7287)
                     /locus_tag="VHV99_27305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007747226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX3639126.1"
                     /translation="MDQDHNKVRFSHNDDRVVVIIGSGAGGGTLANELAQKGINVVVL
                     EAGKSHTQADFTTDEWGSFQMLSWLDKRTTSGTWRIATDFPTLPAWICKTVGGTTTHW
                     AGASLRIKPYEFKAKTTYGDIKDANLLDWPLTGEELDPYYDRAQAKMGVTRTQGLPGL
                     PGNNNFKVMSAGAMKVGYKECNTGHMAINSVVRDDRAHCFQRGFCFQGCRTGAKWSTL
                     YTESPRAQATGHMELRTQAHVVRIETDARGKAKEVLYYDGNGKLQRQKARIVAVAGNA
                     IETPRLLLNSHSSKFPQGLANGSGQLGRNYMRHTTGSVYAVFNDKVDMFKGTTMAGII
                     EDEAVFNPQRGFAGGYHMETVSLGLPFYAAFLDPGAWGPTFSQAMDQYAYTAGMWIVG
                     EDMPRETNRVTLNTDVKDQYGQPVANVHFDDHPNDEAMREHAFKQGTAVYEAAGAKTV
                     YRVPPYPSTHNLGTARMSARAEEGVCNNFGQTHEVSNLFISDGSQFTTGAAENPTMTI
                     VTLAIRQADHIAAQMNKRMV"
     gene            complement(7313..8002)
                     /locus_tag="VHV99_27310"
     CDS             complement(7313..8002)
                     /locus_tag="VHV99_27310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tat (twin-arginine translocation) pathway signal
                     sequence"
                     /protein_id="HEX3639127.1"
                     /translation="MKTTVIPIVDSDHAHHAHHHGHEHDHEHAHKDGHEHRAAKAATA
                     ALRIPLTRRELLRGTGVLMGTLAFSSILSTLAPSRAWALELQGLDTHQGEVILAFTRQ
                     IYPHATLDNAVYALVVKDLDAKAKADPAVRQQLADGVKQLDAKAGSNWLTRTPAAQAE
                     DVAALAGTPFFNTVRSTAVVSLYSNTMAYAHFGYGASEGDGGYLNKGFNSLSWLPNPP
                     PGASGPIPTDS"
     gene            complement(8059..8457)
                     /locus_tag="VHV99_27315"
     CDS             complement(8059..8457)
                     /locus_tag="VHV99_27315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3639128.1"
                     /translation="MAKLIHTMIRVQDLPRSLQFYQNAFNFNVSHRLDFPDFTLVYLR
                     NEEADAEIELTWNKGRTEPYSHGDGYGHVAVCVDDAKRERQRLIDLGMTPNDLREFHN
                     DSGELLARYFFIQDPDGYKIEVLERYGHYQ"
     gene            complement(8815..10476)
                     /locus_tag="VHV99_27320"
                     /pseudo
     CDS             complement(8815..10476)
                     /locus_tag="VHV99_27320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433712.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
     gene            10733..10864
                     /locus_tag="VHV99_27325"
     CDS             10733..10864
                     /locus_tag="VHV99_27325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639129.1"
                     /translation="MGYMMCMYTNDYEETQSLANSQEAEEIDLDAMLTAEEIEILSN"
     gene            10914..>11814
                     /locus_tag="VHV99_27330"
     CDS             10914..>11814
                     /locus_tag="VHV99_27330"
                     /inference="COORDINATES: protein motif:HMM:NF014661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HEX3639130.1"
                     /translation="MQCIVPERSVSLAEAEAKIDLQLRENAVQISLERFGAPLSSTVA
                     TLDSDGFNESCRGCGKGYEDEARVGAKFEAYEHHYSLQYFRAACELMPFPTVAAQPAL
                     AGMLPVRILARNNPTRLGTIRFDDEESLPTLHYPAFLIDYNHPSNRLTGDDADYQLGK
                     RYSCGTGLAAGVGYIEAAIHAVSEVIERHSVGCFIAQHFFYSSRQAVRVVSPDSLPDD
                     VAQVFHDAESCLHSRIQLIDVASAIDCPVYIARCQERHIAGVHVMGAGCSIYAGHAAT
                     RAIKELVQQHHIAEGVEWVRDEWR"
BASE COUNT         2294 a         3619 c         3516 g         2385 t
ORIGIN      
        1 ggccatcaaa gcgtccacga caggttgcga cgatatcgca ttctggctgt attcgtcggg
       61 ctcgaccggc aaaccaaaag gcacggtcca cacgcacgcg aatctgtact ggaccgccga
      121 gttatacgcg aagccgattc tcggcatcgt cgaaagcgat gtggttttct cggcggccaa
      181 actgtttttt gcgtatggtc tcggcaacgg tctgacgttc ccgctttcag ttggcgccac
      241 cgccgtgttg atggccgagc gacccaccgc tgacgccatt tttacgcgcc ttgtgcggca
      301 tcgcccgacc gttttctatg gcgtgccgac gctctacgcg agcatgctcg tgagcccgaa
      361 tctgcccgcg cgcgccgatg ttgcgatgcg cgtgtgcacg tcggcgggtg aagcgctgcc
      421 gcgcgaaatc ggcgagcgct ttacagcgca cttcggctgc gagattctcg acggtatcgg
      481 ctcaaccgaa atgttgcata tcttcctgtc gaatcgcgcg gggcaagttg aatacggcac
      541 cacgggacgc cccgtgcccg gctacgaggt cgaactgcgc gacgaagccg gtcatgctgt
      601 gcccgacggc gaagtcggcg atctgtatat caaagggccg agcgcggcgt tgatgtactg
      661 gagcaatcgc gagaagtcgc gcgcgacatt cctcggcgaa tggattcgca gcggcgataa
      721 gtactgtcgt ctggcgaacg gctgctacgt gtacgcgggc cgcagcgacg acatgctgaa
      781 agtgagcggc cagtatgtat cgcccgtcga agtggaaatg gtattggtac agcacgatgc
      841 ggtgctcgaa gcggcggtgg tcggcgtgga tcacggcggc ctcgtgaaaa cccgcgcgtt
      901 cgtggtgttg aaacgtgaat tcgcaccctc tgagatactt gcggatgagt tgaaggcttt
      961 cgtcaaggag cggctcgcgc cgcacaagta tccgcgcgat attctattcg tcgacgattt
     1021 gccgaaaacc gctaccggta agatccaacg cttcaaactg cgcgaacagt cataaggtga
     1081 tccatgcaga accccgcaac cgcaatctct aacagcgact tcgcagaact gcccgctacc
     1141 gcatcgcatg ggccattgcg catcgaatat cgttggctga atgaagcggc ggctaatgca
     1201 ccgattgccg tattcctgca tgaaggactc ggatcggttg cgatgtggcg cgactggccg
     1261 cagtccttgt gcgaacagct cggcatgcgc gggctcgtct actctcggcc cggttatgga
     1321 ctctccacac cgcgtgaaca cgatgtcaaa tggccggtcg atttcatgac cgctcaagcg
     1381 cgcgatatcc tgcccgcttt gctcgacgca ctcgatatca atatgcagga acgcaagcgc
     1441 atgtggctga tcggccatag cgacggcggc tcgattgcgc tgctctacgc agcgctgcat
     1501 ccagaggcat tggccggcgc ggtaacaatc gcaccgcacg tattcgtcga aaacatctcg
     1561 gtggaaagca tcgcgcagac gaaacagctc tacgaaacaa cagacctgcg cagcaagcta
     1621 agccgctatc acgccgacgt cgattccgcg ttctatggct ggaacgatat ctggttgaat
     1681 cccgcgttta gagaatggcg catcgtcgat gagctgagcg ctatcaggca accgctgctc
     1741 gcagtacaag gccacgacga caactacggc acgatggcgc agatcgacac gattggcgag
     1801 cgtgtgccgc atgcgcaact ggtgaaactc gacgcgtgcg ggcactcgcc gcatcgcgat
     1861 gcgccagttg cgttgaatga ggcgattgcg gcgtttattt cgcggcattg aacatcgtta
     1921 cggcacgccg gcgacggtca cgaacctcac cgggtctcac ccggcctcac cattgcatta
     1981 ccgctccttc acaaaactta cacagatcag cgagtattct cttgcatcgc tgaccttggc
     2041 acgcgaatgc ctcggcgctc cctcccccgt gctggagacg ttccatgtcc gctgtgtcga
     2101 agccgactgc tgctttactg cgcaaacgtt tggcaggtgc actgatcgtc atgtccttat
     2161 gcggcatgct cggtgcctgt ggcaacggcg acgacccatc accagacacg cacgcagcga
     2221 gttccaccga taacggcggc acatcacagg ttctgtcagc gaacccgtcg tccgccgcgc
     2281 ctgcctcacc cgcactaccc gcactgacga ttcaaacccc tgcatcgccc gcatcaagcg
     2341 ccgacacggc tctgtccgca gccgccgcga cgccgctcgc cacacccgtc atccataccg
     2401 tagattgagc tctgcacccc atgcgccacg tggctcacgc tcgcgcgccg catggctcgt
     2461 tcctctcatg aagatcgaaa gccaaaacca tgacatcaaa aaatcgccgt gattttctac
     2521 gctccgccgc gcatgcagcc ggttccgcca ccgcgttgag catgttgccg ctgggcattc
     2581 gcaacgccct cgcgattccc gccaataaca agaccggcag cattcgcgac gtcgagcaca
     2641 tcatcgtgct gatgcaggaa aaccgctcgt tcgaccacta cttcggcacg ctcaagggcg
     2701 tacgcggttt cggcgacacg cgcgcgatca acctgcccaa cggcaagccg gtgtggtatc
     2761 agccgctcgc cgcggatgcc ggttatgtat tgccgttccg cccaacggcg ccgaccctgg
     2821 gcctgcaatt cctgcaggac ctcgcgcacg actggaccac cacgcacgca gcatggaacg
     2881 gcggccgcta cgatcaatgg gtgccatcca agggcaccac gacgatggct tacttcacgc
     2941 gcgacgacat tccgtttcac tatcagctcg ccgacgcctt tacgatctgc gacgcatacc
     3001 attgctcgct attgggcccg accgacccga accgctacta catgtggagc ggctgggtag
     3061 gcaacgacgg caatggcggc ggcccggtga tcgataactc cgaactcggc tacggctggt
     3121 cgacctatcc ggaagtgctg cagaacgcag gcatcacatg gaagatctac caggacatag
     3181 gtacggggct caacgcaagc ggctcgtggg gctggacgca gaacccctac atcggcaact
     3241 ttggcgacaa ctcgctgctc tacttcaacc agtaccgcaa cgcgcaaccg ggcaacccgc
     3301 tgtacgacaa cgcgcgtatc ggcaccaatg cggccaatgg cgacggcttt ttcgacatcc
     3361 tcaagcgcga cgtgcagaac aacgccctgc cgcaagtctc gtggatcgtc gcgccggaag
     3421 cgtattccga gcatccgaac tggccgacca attacggtgc gtggtacatc gatcaggtct
     3481 tgcaggttct cacgtccaat cccgaggtgt ggagcaagac ggtgctgctg atcaactacg
     3541 acgagaacga cggcttcttc gatcacgtgg cgccgccgtt cgcaccggcc tcgagcgcga
     3601 atggtttgtc caccgtcgac accagcaatg aaaactacgc cggtgacagc aaaacacccg
     3661 caggcccgta cggcctcggg ccgcgcgtgc cgatgctggt agtctcgccg tggtcgaaag
     3721 gcggctacgt gtgctcggag ttgttcgacc atacttccgt gatccgcttc atcgaaaagc
     3781 gcttcgggca gcagcacaac ctcggcgaat cgaacatcac gccgtggcgt cgcgcggtgt
     3841 gcggcgatct gatgtcggca ttcaacttca gcaacccgaa cgacgcgttc ccggcgctgc
     3901 cgagcacgag cggctatgta ccgcccgatc agaaccggca tccggactat gtgccgttgc
     3961 cgccgctcct gcaggccgtg ccgaagcagg aacccggcgt gcgcccggcg cgcgcgttgc
     4021 catacgaatt gttcgtgcgc gtgcaaggcg aaggctccgg caacaagctg acaatgcgtt
     4081 tcgtcaattc gggccgcgcg ggcgcggtgt ttctcgtcta tgcagcgaac ggtctcgacg
     4141 caccgcgtac gtatacggtc gaagccggca agcagttgca ggattcgttg ccattgaatg
     4201 ccgacggcac gtacgatttc accgtgcacg gtccgaacgg ttttctgcgc cgctttgccg
     4261 gcaaaccggt cgcgagaaac tggtggaacg gttccgatat tgcgcgcccg gacgttgccg
     4321 aaggctacga cgtcgcgaac ggcaacctgc aattgcgact ggcgaacgcg ggcagcgcgc
     4381 gctgccagtt cacgatcgtc aacgcctatg acgcgaacaa cgtgatcaag cattcggtgc
     4441 gcggcggcga taccgaacag ttgtatctcg atctgcgcaa cgcccatggc tggtacgatc
     4501 tggcgattac cgtcgacacg gacccgtcgt tcgcgcgccg cttcgccgga cacgtggaaa
     4561 ccggcgagag cagcatgagc gatccggcat tgggaagcta agcttcaacc gcgtttcaat
     4621 cgcgggcgag acactcgacc atccgcatca tctacattct ccggtgatgt gagtgtccgc
     4681 ccggatcaac gcggcggcgg cctaagactc gaagtcgagg ccgccgatca aaaccgccgg
     4741 ctcggcctgt gtgtgcgaaa gaaaatccag ttcgcgcaca tggcgccagg cttcgagttt
     4801 gcgcggcaac gccgccagat agccggcgaa gcgcgccgcc cctgcggaac ccatcagccg
     4861 ttcgatctcg tcgccgtccc aggtcagaaa cagattcaat ggatgcgggt agtggcgcag
     4921 gtcttcgatc ggcgttgcat gaatccgcac gcgggtgggg tgtccatgcc cgccataagg
     4981 cagcacttcg gtatgcacgc tagtggcgaa acaggcggcg agcgcctcgg cgatccttgg
     5041 cgcaaactgt ttatcgaaac gggcggcact ctctggacgc atggatgtct ccgacgttct
     5101 tcacaacgag cgaagcatcg tagcatgcga aaccggccgt acttaccgct gacgcggcgc
     5161 gcgcaatgtt cagacgcggc gtgcgcgcaa gccgtttggg catcagacct tcttcagaat
     5221 ccgcggcctg gtctcgttga ccaccgcggc cggttccggc gtggcatcgg acacgcttgt
     5281 ggccttttcc gttcgcatcg acagatagcg caaacagcac acccgcagga acgatgcaaa
     5341 gttggtcggc atctcgccac gcagcgcgat gagttcatcg tatagcttca ccgcgaactg
     5401 actggtcgtc atattctcgc gcgcagcaat ctcgtgcagc acatcccaga acagattctc
     5461 cagccggacc gtcgtgatca caccgtgaat ccgcagcgag cgggtgcgcg attcatagag
     5521 aatcggatcg gcgttgatgt aaattccaca catggtcatt actccctgtc taccggcgca
     5581 cgtgccacgg cttgcttcat tgcgccgcgg cacgccgccc tccgtcacca acatacgccg
     5641 gcatccacca ctcgaccatc gtatcgaagc cgaacggcgc acctggctca cccacccgcc
     5701 ttacaccatc cgcttgttca tctgcgccgc aatgtgatcg gcctgacgga tcgctagcgt
     5761 cacgatcgtc atggtcgggt tttccgccgc gccggtggtg aactggctgc cgtccgagat
     5821 gaacaggttc gacacctcat gcgtctgccc aaagttgttg cacacaccct cttcggcacg
     5881 cgcactcatc cgcgcggtcc cgaggttgtg ggtggacgga tacggcggta cgcggtacac
     5941 cgtcttcgcg cccgcggctt catagaccgc cgtgccctgt ttgaacgcat gctcgcgcat
     6001 cgcttcgtca ttcgggtgat cgtcgaaatg cacgttggcc accggctggc cgtactgatc
     6061 cttcacgtcg gtattcaagg tcacgcgatt ggtctcgcgc ggcatatcct cgccgacgat
     6121 ccacatgcca gcggtgtacg cgtactgatc catcgcctgg ctaaacgtcg gcccccatgc
     6181 gcccgggtcg agaaaagccg cgtaaaacgg cagaccgagc gaaacggtct ccatgtgata
     6241 gccgcccgcg aagccgcgtt gcggattgaa caccgcttcg tcttcgatga tgccggccat
     6301 cgtcgtgccc ttgaacatgt cgaccttgtc gttgaacacc gcatacaccg agccggtggt
     6361 gtggcgcatg taattacgtc ccagctgccc cgagccgttc gcgagccctt gcgggaattt
     6421 gctcgaatgg gaattcagca gcagacgcgg tgtttcgatc gcattgcccg ccaccgcgac
     6481 gatgcgtgcc ttttgccgct gcagcttgcc gttgccgtcg taatagagaa cttcctttgc
     6541 cttgccacgt gcatcggttt cgatacgcac cacatgcgcc tgcgtgcgca actccatatg
     6601 gcccgtggcc tgtgcgcgcg gcgattcggt gtagagcgtg gaccatttcg cgccggtacg
     6661 gcagccctga aaacagaagc cgcgctggaa gcaatgcgcg cgatcgtcac gcaccacact
     6721 gttgatcgcc atatggccgg tattgcactc cttgtagccg accttcatcg cgccggcgga
     6781 catcaccttg aagttgttat tgcccggcag gcccggcaga ccctgcgtgc gcgtcacacc
     6841 catcttcgct tgcgcgcgat cgtaatacgg atcgagctct tcgccggtga gcggccagtc
     6901 gagcaggttg gcatccttaa tatcgccgta ggtcgtcttc gccttgaact cgtagggttt
     6961 gatacgcaga ctggcgcccg cccagtgcgt ggtcgtgccg cccaccgtct tgcagatcca
     7021 tgcgggcaga gtcggaaaat ccgtcgcgat gcgccatgtg ccggacgtag tgcgtttgtc
     7081 gagccatgac agcatctgaa acgagcccca ttcgtccgtg gtgaaatcgg cttgcgtatg
     7141 cgatttgccc gcttccagca cgacgacgtt gatgcccttc tgcgccagtt cgttggccag
     7201 cgtgccgccg cccgcacccg aaccgatgat cacgacgacc ctgtcgtcgt tgtgcgaaaa
     7261 acgcaccttg ttgtggtctt gatccatgtt ctgtctccgc tgttctggct atttagctat
     7321 ccgtcggaat cgggccgctg gcgccgggcg gcggattggg cagccacgag aggctgttga
     7381 aacccttgtt caggtagccg ccatcgccct cggaagcgcc atagccgaaa tgggcatacg
     7441 ccatggtatt gctgtacagc gagaccacgg ccgtgctgcg caccgtgttg aaaaatggcg
     7501 tccccgcgag cgctgcaacg tcctcggcct gtgcagccgg cgtgcgcgtc agccagttgg
     7561 aaccggcttt cgcgtcgagc tgtttgacgc cgtccgcgag ctgctgacgc accgccgggt
     7621 ccgccttcgc cttggcgtcg aggtccttga ccacaagcgc atagaccgcg ttgtcgagcg
     7681 tggcgtgcgg gtagatctgg cgcgtgaagg ccagaatcac ttcgccctgg tgtgtgtcca
     7741 gaccctgcag ttcgagcgcc catgcgcggc tcggcgcgag cgtcgacaat atcgatgaga
     7801 acgccagcgt gcccatgagt acgcctgtgc ctctcaacag ttcgcgtcgc gtaagtggaa
     7861 tccgcaacgc cgcggtggca gccttcgccg cgcgatgctc atggccgtct ttatgagcgt
     7921 gctcgtggtc atgctcgtga ccatgatgat gcgcatgatg ggcgtgatcc gaatcgacga
     7981 tcgggatgac cgttgtcttc atgtctgtct ccttgtgggg tattgcaact acgcgcggcg
     8041 gccgcctggt ctggattgct attgatagtg gccgtagcgt tccagcactt cgatcttgta
     8101 gccgtcaggg tcctgaatga agaaatagcg cgccagcaac tcaccgctgt cgttatggaa
     8161 ttcgcgcaga tcgttgggcg tcatgccgag atcgatcagc cgctgacgtt cgcgctttgc
     8221 atcgtcgaca cagacggcca cgtggccgta gccgtcgcca tgcgaatagg gttcggtgcg
     8281 gcctttattc caggtcagtt cgatttcggc gtcggcttct tcattgcgca aatagacgag
     8341 tgtgaaatcg ggaaaatcga gccggtgtga aacgttgaag ttgaacgcgt tctgatagaa
     8401 ctgcaacgag cgcggcagat cctgcacgcg aatcatcgtg tggatgagtt tggccatcgg
     8461 tgtctcctcc tctttcaatc aagtgtagga ggctgctcac gcgaatggta atgcacggct
     8521 aaatcgtgcc agggacttgc cctatgctgc acgcgcgtcg ccggcgaggt ttgcgcacgc
     8581 gcgtgactgg cgtgagcggc aatggacgcg gtcccgcggc aagttgcaat gcagcacgcg
     8641 gcacacggca agcggcaagc ggcaagcggc aagcggcaag cggcaagcgg cgcagactct
     8701 atgcccgcaa gctgaatggc gttaggcgaa acccggcatc ggctccagat caacgcgtgc
     8761 tcgttgaagc gcattcgtat ttgccgggaa gcgtgcacgc gtcgtcatcg ctattcaggg
     8821 tttggtggcc ggattgtccg cgtcttgccc gagtccctcg cgcaatgctt tgcgaatcac
     8881 cttgccggtg gcggtcattg gcaggctttc gacgaagcgg atttcgcgcg gatattcgtg
     8941 tgcggcgagg cgcgttttga catgctgctg aatctcgcgt accagtgcct cgtcgccgac
     9001 gaagccgggc ttcagcacca cgaacgccat cacgatctcc gtgcgctcgc ggtccggcgc
     9061 gccgatcacc gcggccatcg ataccgccgg atggcgcagc aatgaatcct cgatcgaagc
     9121 cggcccgatg cgataaccag cgctcgtgat cacgtcgtcg ccacggccga cgaaacggat
     9181 gaagccgtcc gcatcgcgtg tcccgaggtc gccggtgacc agaaacctgc cacggaattt
     9241 ttcgcgggtg gccggctcgt tgccccagta gccgaggaac atgaccgggt caggcgcggc
     9301 taccgcgata tcgccgatcg cgccttgcgg cagttcgttg ccgtcggcgt cgatgatcgc
     9361 cacatgatgc ccgggcaccg cgcggccaat cgcgccgaag cacggctcga acaacgccgc
     9421 acatgacgac accacgacgt tgcactcggt ttgtccatag aactcgttga tcgtgacgcc
     9481 cagcgccttg cgtccccagc cgatcaattc ctcgccgagc gattcaccgc cgcttgccac
     9541 cgagcgcaac gcgagcgtcc attgttcggg acgcttgacg ccgcgcatca tcttgagcgc
     9601 ggtgggcggc aggaaagtat gcgagaccgc gtgacgcgcc atcagatcga aggcggcctg
     9661 gccgtcgaat tttgcaaagc ggcgcgcaag cacagcgacg ccgtgatgcc atgacggcag
     9721 cagcacgtcg aatagcccgc cgatccaggc ccagtccgca ggtgtccaca tcagcgtggc
     9781 gtgcgcgggg aagccctgct gggacagctc gacacccggc agatggccgg gcaacacgcg
     9841 gtgcccgtgc aaggcgccct tcggtttgcc ggtggtgccc gatgtgtaga tgatgaccgc
     9901 cgggtcgtca gcggcggtgt cggccggtgt gaagtcggcg gatgcgctgt tgagtgcttg
     9961 ccagaaggaa cgcgctgggg cttgctgatc gtggttgtca ctgtctatgt cgacgctgaa
    10021 cacggtgcgc agtgcaggca aggcgttgcg gatttcgtcg attttttgca cgcctgcgtg
    10081 gtcggtgatc agtgcgactg cgccgctgtc gccgagacgg tgttcgattg cgtcgacgcc
    10141 gaataatgca aagagaggca cggctatcat gccggctttg taagcggcga ggtgggcgat
    10201 ggccgtttcg atcgattacg acaggaagat gccgatgcgg tcgcctcgct gtgcgccgga
    10261 tgcgagtagc gcgttggcga agcggtcaga cagcgacttc aattcgtcga atgtatagcg
    10321 ggtggctttg ccttcggggt cttcgtagat cagggcgagg cggcccgagc cgtcggccca
    10381 tttgtcgcat acgtcgacgc cgatgttgta gcgcgccgga atgcgccagg tgaagttgac
    10441 cttcagcgat tcgtaggtct ggccgtcgag gttcatggtg tctccgtcgc ttgatgcttt
    10501 cttcgtgatt tttttgagtc tagctcaacg ggttggggcg tgttgaggga ggtccccttt
    10561 actcgacgtt aatccgcgcc atgaagtcgc agggttatcg atcattacat attcaattca
    10621 catttattac tgtttaatta ctgtcatccg acagcgtggc atcgccgaat agcgcatcac
    10681 gcgcaatcgg cgcgtaacgg tccgggttct ctaaatccaa tcggaggtag ctatgggcta
    10741 catgatgtgc atgtacacga acgactacga ggaaacccaa tcactggcga actcacaaga
    10801 agctgaggag atcgaccttg atgcaatgct aactgcggag gagatcgaaa ttctttccaa
    10861 ctaagtcagg aaagggggtg aattcgcgcc ccctttttaa atggagtcta tccatgcaat
    10921 gtatcgtacc ggaacgttcg gtttccctcg ccgaggctga agcgaaaatc gatctccaac
    10981 tgcgtgaaaa cgctgtgcaa atcagcctgg agcgcttcgg cgcgccactt tcaagcacgg
    11041 tcgcgaccct ggactcagat ggattcaatg aaagttgccg aggttgtgga aaaggatatg
    11101 aagacgaggc tcgggtcggt gcgaaattcg aagcgtacga acatcactat tctctgcaat
    11161 attttcgagc cgcttgcgaa ctcatgccct ttccaacagt agcagcacaa ccggccttgg
    11221 ctgggatgct gccagtccgg attcttgcca ggaacaatcc aacccgactt ggcacgatac
    11281 gatttgacga tgaagaatcc ctccccactc ttcattaccc cgcttttctg attgactaca
    11341 accatccatc aaatagactc accggggatg acgctgacta tcaacttggc aaacgctatt
    11401 cgtgcggcac cggcttggcg gctggagtgg ggtatatcga agccgccatt catgcggtga
    11461 gtgaagtgat cgagcggcat agcgttggat gctttattgc tcagcatttc ttctactcct
    11521 cccggcaggc cgttcgggtt gtttcgccag attcattgcc cgatgacgtt gcccaggtat
    11581 ttcacgatgc cgagtcatgc ctgcattcca gaatccagtt gattgacgtg gccagtgcga
    11641 tcgattgtcc agtctatatc gcacgctgcc aggaaagaca tattgcaggt gttcacgtga
    11701 tgggagcggg ttgctcgatc tatgccggtc acgcagcaac gcgggcgatc aaggagttgg
    11761 ttcaacaaca ccacattgct gaaggtgtcg agtgggtgcg cgacgagtgg cggc
//
LOCUS       DATASH010001029         4940 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5319290,
            whole genome shotgun sequence.
ACCESSION   DATASH010001029 DATASH010000000
VERSION     DATASH010001029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4940
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5319290"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..161)
                     /locus_tag="VHV99_27335"
     CDS             complement(<1..161)
                     /locus_tag="VHV99_27335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX3639131.1"
                     /translation="MKALAKLERGPGLTLTRVKKPEVGHNDVMIRITRTAICGTDIHI
                     WKWDEWAQKT"
     gene            complement(187..1386)
                     /locus_tag="VHV99_27340"
     CDS             complement(187..1386)
                     /locus_tag="VHV99_27340"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEX3639132.1"
                     /translation="MHDSYLAHVRSTLDQIRADGFYKSERVIASPQSADIRLADGGEM
                     LNFCANNYLGLANDARLIEAAKQGLDRDGFGMASVRFICGTQTVHKELEQALATFLKT
                     DDCILYSSCFDANGGLFETLLDESDAIISDELNHASIIDGVRLSKAKRYRYKNNDLAD
                     LETKLREADAAGARFKLIATDGVFSMDGIIANLAGICDLADRYGALVMVDDSHAVGFI
                     GEHGRGTPEHCGVLSRIDIITGTLGKALGGASGGYVAARREIVELLRQRSRPYLFSNT
                     LTPSIAAATLKVLDLLASDEGARLRASVRANGAHFRDAMSKLGFTLVPGEHPIIPVML
                     GDAQVASRMADALLHEGVYVIGFSFPVVPKGRARIRTQMSAAHTPAQIDRAVDAFARV
                     GRSLGII"
     gene            complement(1524..2498)
                     /locus_tag="VHV99_27345"
     CDS             complement(1524..2498)
                     /locus_tag="VHV99_27345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006408165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HEX3639133.1"
                     /translation="MSGVGMILDSLRLANEIDGHALYDWQISSWDGRPLTFAGGAQLH
                     ADTAFGDAISCDWLIVVSERYQQFADYRLFLQSLARVGSRTPRVTGIHHGVWWLAMAG
                     QLSAYRVAVNWETYQQFAEEFERSIVTQQIFEIDRDRSTCTGGQATVDFMLAMIARDQ
                     GADLAERIADSLGIGVLRSGEERQRIPFVTAPGERHPKLNDALQLMEANIEDPLATDE
                     IAGLVGISRRQLERLFRQYLGAMPSKYYVNLRLTKARTQLQRTSKSVVQISLACGFAS
                     AAHFSNAYRDRFGVTPREERRNWIEKHSGQAPDEPRGGALLGSSREPV"
     gene            complement(2590..3381)
                     /locus_tag="VHV99_27350"
     CDS             complement(2590..3381)
                     /locus_tag="VHV99_27350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3639134.1"
                     /translation="MEPTAKDAATKLVAQDIHKRYGDNEVLKGVSLSARAGDVISIIG
                     ASGSGKSTFLRCINFLERPNAGQIEVDGETVQVKADRAGNYEVADRKQLQRVRTKLAM
                     VFQHFNLWSHMTAIENVMETPRHVLGLSKKEAEERARMYLEKVGLAPRIEKQYPSHMS
                     GGQQQRVAIARALAMHPDVMLFDEPTSALDPELVGEVLKVMQKLAEEGRTMIVVTHEM
                     GFARNVSNHVMFLHQGRTEEEGAPADVLTAPKSERLRQFLSGSLK"
     gene            complement(3488..4201)
                     /locus_tag="VHV99_27355"
     CDS             complement(3488..4201)
                     /locus_tag="VHV99_27355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3639135.1"
                     /translation="MIQILADYWRPFLYSDGMRASGLVVTLWLLASSLALGLVAAVPL
                     ACARVSKNRWLATPVRLYTYVFRGTPLYVQLLLLYTGMYGLEFVRSQSLLEAFFRSGF
                     NCAILAFALNTCAYTTEIFAGAIRSIPYGEVEAARAYGMSTFTVYRRVILPSALRRSL
                     PLYSNEVILMLHATTVAFTATVPDILKVARDANSATYQSFESFGLAALIYLAVSFVLI
                     GAFRRAEKRWLGYLGASRR"
     gene            complement(4258..>4940)
                     /gene="hisQ"
                     /locus_tag="VHV99_27360"
     CDS             complement(4258..>4940)
                     /gene="hisQ"
                     /locus_tag="VHV99_27360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004527442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HEX3639136.1"
                     /translation="YGYGPLLLAGAKQTIALAVLSLAVSILLGLAGASAKLSGNRVTR
                     SIATAYTTLIRAVPDLVLMLLLFYSIQIAVNNLTDALGLEQFDIDPFTAGVLTLGFIY
                     GAYFTETFRGAFLAVPRGQLEAGSAYGMSGARVFVRILFPQMMRFALPGIGNNWQVMI
                     KATALVSIIGLADIVKAAQDAGKSTYKMFFFIMVAAAAYLAITTVSNLVLLYLERRYS
                     TGVRHADL"
BASE COUNT          977 a         1506 c         1532 g          925 t
ORIGIN      
        1 gtcttctgcg cccactcgtc ccatttccag atatggatgt cggtgccgca gatcgctgtg
       61 cgcgtgatgc ggatcatcac gtcgttatgc ccgacttcag gcttcttgac gcgcgtcagc
      121 gtcagccccg ggccacgttc cagttttgcg agtgctttca tgacttgtcc gatttgtccc
      181 gatgtttcag atgatgccca gtgaacggcc gacgcgcgca aacgcatcga ccgcgcgatc
      241 gatctgcgcc ggcgtatgcg cggcgctcat ctgcgtgcgg atacgggcgc ggcctttcgg
      301 caccacgggg aacgaaaacc cgatgacgta gacgccttca tgcagcagcg cgtccgccat
      361 gcgcgacgcg acctgggcgt cgcccagcat caccgggata atcggatgtt cgccgggcac
      421 gagcgtgaaa ccgagcttgc tcatggcgtc gcggaaatgg gcgccgttcg cgcgcacgct
      481 ggcgcgcaac cgggcgcctt cgtcgctcgc cagcaggtcg agcaccttga gcgtggcggc
      541 ggcaatactg ggtgtcagcg tattcgagaa cagatagggg cgcgagcgtt gccgcaacaa
      601 ctcgacaatc tcacggcgtg cagccacata accgccggac gcgccgccca gcgccttgcc
      661 taatgtgccg gtgatgatgt cgatgcgaga cagcacgccg caatgctccg gcgtgccgcg
      721 tccatgctcg ccgataaagc ccaccgcgtg cgagtcgtcg accatgacca gcgcaccata
      781 tcgatcggcg agatcgcaga tgccggcgag attcgcgatg atgccgtcca tcgaaaaaac
      841 gccgtcggtg gcgatcagct tgaagcgcgc gcccgcggcg tccgcttcgc gaagcttcgt
      901 ttcgaggtcg gccagatcgt tgttcttgta gcggtaacgc ttggccttcg acaaacgcac
      961 gccgtcgata atgctcgcgt gattgagttc gtcgctgatg atggcgtcgg actcgtcgag
     1021 caaggtttcg aacaggccgc cgtttgcgtc gaagcaactc gagtacagga tgcagtcgtc
     1081 agttttcagg aaggtggcga gcgcttgctc gagttccttg tgaacagtct gcgtgccgca
     1141 gataaaccgc actgacgcca tgccaaaacc gtcgcggtcg aggccttgtt tggcggcttc
     1201 gatcaggcgc gcgtcgttgg caagaccgag gtaattgttc gcgcagaagt tcagcatttc
     1261 gccgccatcc gccaggcgaa tgtcggccga ttgcggactc gcgatcacgc gctcggactt
     1321 gtaaaaaccg tcggcgcgaa tttgatcgag cgtgctgcgc acatgcgcaa gataagaatc
     1381 atgcatggat catgctccta agtaatacga cgaccgcacg gcaacgccgt ttcggtgctg
     1441 cggctgccag tatgaaggaa aattccattc acgcgaacat tcgcgggcac tgaaacggag
     1501 ttggcaagct ggcacaaagg tcttcaaacg ggctcacgcg atgaaccgag taacgcgccg
     1561 ccacgcggtt cgtcgggcgc ttgtccgcta tgcttttcga tccagttacg gcgctcctca
     1621 cggggcgtca cgccgaagcg gtcgcggtat gcattggaga aatgcgccgc cgatgcaaac
     1681 ccgcacgcga ggctgatctg caccaccgac ttgctggtgc gctgcaattg cgtgcgtgct
     1741 ttggtgaggc gcaggttgac gtagtacttc gatggcatcg cgccgaggta ctgtcgaaac
     1801 agccgttcga gctggcgtcg cgagatgccg acaagcccgg cgatttcatc ggtggcgagc
     1861 gggtcttcta tgtttgcttc catcagttgc agcgcgtcgt tgagcttcgg atggcgttcg
     1921 cccggcgcgg tcacgaacgg aatgcgctgg cgttcttcgc ccgagcgcaa tacgccgatg
     1981 cccagcgaat cggcgatccg ttccgccaga tccgcgccct ggtcgcgcgc gatcatcgca
     2041 agcatgaagt cgacagtcgc ctggccgccg gtgcaggtcg aacgatcgcg atcgatttcg
     2101 aagatctgct gcgtgacgat cgaacgctcg aactcctcgg cgaattgctg gtaggtttcc
     2161 cagttcaccg ccacgcggta cgcgctcaac tggcccgcca ttgcgagcca ccagacgccg
     2221 tggtgaatac cggtcacacg tggcgtgcgg ctgcccacgc gcgcgaggct ttgcaggaat
     2281 aaccgatagt ccgcgaattg ctgataccgc tcgctgacca cgatcagcca gtcgcagctg
     2341 atggcgtcgc cgaacgcggt atcggcgtga agctgcgcgc cgcccgcgaa tgtcaggggc
     2401 cggccgtccc acgagctgat ttgccagtca tagagcgcgt ggccgtcgat ctcgttcgcg
     2461 agacggaggc tgtcgaggat catgcccacg ccgctcatgg agcagggagg cagcaccacg
     2521 actgcgacct tgagtggctt ggcgccgggg ccggagttaa cgttaacgtc cgcgcttatg
     2581 ctcacgtgct tacttaagac taccggaaag aaactgtcgc agacgttcac tcttcggcgc
     2641 cgtgagcacg tctgccggcg caccttcttc ctcagtgcgc ccctgatgca ggaacatcac
     2701 atggttcgag acattgcgcg caaagcccat ctcgtgcgta accacgatca tcgtgcggcc
     2761 ctcttcggca agcttctgca tcaccttcag cacttcaccg accagctccg gatcgagggc
     2821 ggaagtcggt tcgtcgaaca gcattacgtc gggatgcatt gccagtgcgc gcgcaatggc
     2881 cacgcgctgc tgctgcccgc cggacatatg cgaaggatat tgcttctcga tgcgaggcgc
     2941 gagaccgacc ttctcaaggt acatccgcgc acgctcctcc gcctctttct tcgaaagacc
     3001 cagcacatgt cgcggcgttt ccatcacgtt ttcgatggcg gtcatatgcg accacaagtt
     3061 gaagtgctgg aacaccatcg cgagcttcgt gcgcacacgc tgcaactgct tgcgatcagc
     3121 cacctcatag ttgcccgcgc ggtcggcctt gacttgcacc gtctcgccat ccacctcgat
     3181 ctgccctgcg ttcggccgct caaggaagtt gatgcagcgc aggaacgtgc tcttgcccga
     3241 gccactcgcg ccgatgatgc tgatgacgtc gcccgcacgt gcggaaagcg atacgccctt
     3301 gagcacctcg ttgtcgccgt agcgcttgtg aatgtcttgc gcgaccagct tcgtcgcggc
     3361 atctttggcg gttggctcca actcatactc ccggggtggt tgactgaact gcaattcgaa
     3421 ccagggtaac gctctagctg gccgatttta aacgatgatc tcaagcgacg acctctaacg
     3481 acgacctcta gcgccgactc gcgcccagat atccaagcca gcgcttttcc gcccgccgga
     3541 acgcgccgat caatacgaac gacaccgcca ggtagatcag cgccgcgagc ccgaaggact
     3601 cgaacgattg ataagtggcc gagttggcgt cgcgcgcgac cttcaggatg tccggtacgg
     3661 tggccgtgaa cgcgaccgtc gtggcgtgca gcatgaggat cacttcgtta ctgtacagcg
     3721 gcagcgagcg tcgcaacgcg gacggcagga tcacgcgccg gtacaccgtg aaggtgctca
     3781 tgccgtacgc gcgtgccgct tccacttcac cgtacggaat cgagcggatc gcaccggcga
     3841 aaatctcggt ggtgtacgcg caggtattga gcgcgaacgc caggatcgcg cagttgaacc
     3901 cgctgcgaaa gaaggcttcg agcagcgact gcgagcgcac aaactcgaga ccgtacatgc
     3961 cggtgtagag cagcagcaac tgcacataaa gcggcgtgcc gcgaaacacg taagtgtaaa
     4021 ggcgcaccgg agtggcgagc cagcggttct tcgatacgcg cgcgcaggca agcggaaccg
     4081 ccgcgacaag accgagcgca agcgaactcg ccaacagcca caaggtgacg acgagacccg
     4141 aggcgcgcat gccgtcggag taaagaaacg ggcgccagta atcggcaagt atctggatca
     4201 tgatggtcgc gttcgccggt ttgctgagag gtagctcaga ggtagctgag agttggctta
     4261 gaggtcggcg tgccgcacgc ccgtggaata gcggcgttcg agatacaaca gaacgaggtt
     4321 cgagacagtc gtgatggcga gataggctgc ggccgccacc atgatgaaga aaaacatctt
     4381 gtaggtgctt ttgccggcgt cttgcgcggc cttgacgata tcggcgagac cgatgatcga
     4441 caccagcgcc gtcgccttga tcatcacttg ccagttgttg ccgatccccg gcagcgcgaa
     4501 gcgcatcatc tgcggaaaca ggatgcgcac gaacacacgc gcgccgctca ttccataggc
     4561 gctgccggct tccagttggc cgcgcggcac cgcgagaaac gcgccacgaa aggtttcggt
     4621 gaagtaggcg ccgtagatga agccgagcgt gaggacgccg gccgtgaagg gatcaatgtc
     4681 gaattgctcc aggccgagtg catcggtcag attgttgacc gcgatctgaa tgctgtagaa
     4741 aagcagcagc atcaggacca ggtcgggcac ggcacgaatc agcgtggtgt aggcggtggc
     4801 gatgctgcgc gtgacccggt tgcccgatag cttggcgctt gcaccggcga ggccgagcag
     4861 gatggatacc gcaagagaca ggaccgcgag cgcaatcgtc tgtttcgcgc ccgccagcag
     4921 cagtggacca tagccgtata
//
LOCUS       DATASH010001030         6848 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2550158,
            whole genome shotgun sequence.
ACCESSION   DATASH010001030 DATASH010000000
VERSION     DATASH010001030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6848
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2550158"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="VHV99_27365"
     CDS             <1..808
                     /locus_tag="VHV99_27365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639137.1"
                     /translation="ITMTAIALPTITMATIAMTTITMTAIALPTITMATIAMTTITMT
                     AIALPTITVAAMVTMAAVMPTAITMAACPTVTAVTIAACAACPTFTIAARAASAAITR
                     PTTTTTTTAAPTATAPPTPGTESCPAASTTVSARPVRRSPAAIAALRARSRRRPLTRS
                     RRCHANSGGRPQSTVFRLAAKRVEKRTVDRQCLRDHQLRRIRRRRHSCCRTRLRACHR
                     RIKTHSNRQYRSACPQRPRVPVLLFHFILLIMYSEKQIQITSESLFPLKK"
     gene            1487..2248
                     /locus_tag="VHV99_27370"
     CDS             1487..2248
                     /locus_tag="VHV99_27370"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013689269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEX3639138.1"
                     /translation="MLLSEQWVLVTGGARGLGAAITRALLREGAGVVVNYRRSETLAH
                     ALREEFGDRVLPIQADVIDATAIARLFETAQAQTGQPIVSVVNNALADFQFDGDARPK
                     LDSIDWARFQQQLDGSLKGALNTMQAAAAGMRSRGFGRIVNVGTNLFQNPVVPYHDYT
                     AAKAALLSLTRTAAHDLGPDGITVNMVSGGLLRTTDASAATPEFVFDLIASSTPMRRV
                     TTPEEFADAVLFFLSPWSRAVTGQNLIVDGGLVKG"
     gene            2556..3944
                     /locus_tag="VHV99_27375"
     CDS             2556..3944
                     /locus_tag="VHV99_27375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX3639139.1"
                     /translation="MVSVSSSAVEELKAAVRGQLLRPGDAGFDEARSIWNAMIDRHPA
                     MVLRCAGAADVRRGVAFARDNGLPLAVRGGGHNIAGTALCDDGLVIDLSLMKSVHIDA
                     AARRAYVEPGATLRDFDHEAQAFGLATPLGINSTTGVAGLTLGGGFGWLSRKYGMTVD
                     NLISADVVTADGELVHASAESHPDLFWAIRGGGGNFGVVTRFEFALHAVGPLVYGGLV
                     VLPIEQAKNALLQYRTAAEQMPDDLSVWVVSRLAPPLPFLPPEAHGKPVIVFAMCYTG
                     PVENGPAVVQAVRGFGTPIGEHLGPMAYAAWQQAFDPLLTPGARNYWKSHNIGKISDG
                     LIDALLDAIGKLPSPQCEIFFGQIGAQTSRVPVEATAYSSRDTQYAMNVHGRWDDASD
                     DERCIGWARAFFDASAPFALGSVYVNFMTQEESGRVADAYGPNYERLVAVKKHYDPHN
                     LFRHNQNIRPAA"
     gene            4139..4729
                     /locus_tag="VHV99_27380"
     CDS             4139..4729
                     /locus_tag="VHV99_27380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008924270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HEX3639140.1"
                     /translation="MRRRQFIMTTSAALATAGFSLAGCTTTSPSSSASPSANAGKRDT
                     INAGVDSTLSRLYANVAGSRELVGKARGVLVFPSVISAGFWVGGQYGEGALRVAGRSS
                     GYYSTVAGSFGLQIGAQSKALVFLFMTQEALDRFLGSQGWAAGADATVALLKIGANGA
                     VDTSTATSPVEAFVLTNGGLMAGVSLEGTKVSRLII"
     gene            5174..6676
                     /locus_tag="VHV99_27385"
     CDS             5174..6676
                     /locus_tag="VHV99_27385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEX3639141.1"
                     /translation="MNTASPTPRWLAKLPGIKSNVLAGLTSSFALVPECIAFALVAHL
                     NPLMGLYGAFFICTITALFGGRPGMISGAAGSMAVVIVALVVQHGAQYLLATVILSGI
                     LMVLFGALRLGKLIRMVPHPVMLGFVNGLAIIIAMAQLAHFRQSTPQGEQWLHGSALL
                     LMCGLVALTMLIVYLLPRLTRAAPPALVAIVGVGLFSQLSHLPARTLGDMAHIAGGLP
                     SLHVPRVPLNLDTLHVVLPYAVLMAIVGLLETLLTFNLTDEITDTRGQPNRECLALGA
                     ANIASGLFGGMGGCAMIGQTMINLNSGGRSRLSGIVSGVMILMFILFLSPLIERIPLA
                     ALVGVMFVVAQQTFAWGSLRVLGKVPRNDALVIVAVTTITVFSDLAIAVLCGIVIAAL
                     NFAWQHAREIHAHVEDHGGDKTYVPRGTLFFASTSRFHELFDPAKDPGHITVDCRHLT
                     LADHSALAALHGLFERYRKAGKQLRVENLSERNQRLLSRAGIEISGRGAA"
BASE COUNT         1246 a         2254 c         2003 g         1345 t
ORIGIN      
        1 catcaccatg accgccatcg ccctgcccac catcaccatg gccaccatcg ccatgaccac
       61 catcaccatg accgccatcg ccctgcccac catcaccatg gccaccatcg ccatgaccac
      121 catcaccatg accgccatcg ccctgcccac catcaccgtg gccgccatgg tcaccatggc
      181 cgccgtgatg cccaccgcca tcaccatggc cgcctgtccc accgtcaccg ccgtcaccat
      241 tgccgcctgt gccgcctgtc ccactttcac cattgccgcc cgtgccgcca gtgccgccat
      301 cacccgtccc accaccacca ccaccacaac cgccgccccc accgccaccg ctccccccac
      361 tcccggcacc gagtcctgcc ccgccgcctc caccaccgtt agcgcccgcc ccgttcgacg
      421 aagtcccgcc gccattgctg ctctgcgagc ccgaagccga cgtcgacccc tgacccgtag
      481 ccgccgctgc cacgccaatt ccggcggacg cccgcaatcc accgtgttcc ggcttgcagc
      541 caagcgtgtc gaaaagcgga cagtcgaccg gcaatgcttg cgcgaccatc agcttcgtcg
      601 aattcgccga cgacgccaca gctgctgccg gactcgtttg cgcgcctgcc atcgccgcat
      661 aaagactcac agcaaccgcc aataccgctc ggcttgtccc caacgcccgc gcgtccccgt
      721 tttgcttttt catttcattc tcctaatcat gtattctgaa aaacagatcc agataacgag
      781 tgaatccctg ttcccgttaa aaaagtgaaa cttcaaagcc cggtcgacat tgcggcgtgc
      841 cacacgaaaa cgcgcggcac cctgcatctc tcccgcagat aaccccggtc cgatttgacg
      901 tgattcggga gagacaactg gctgcgcgcg aacaaagtct tgagagggca cagcagggcc
      961 tgatggacgt tgaagttatg cgcagcgcca ccaggtgacc gtgcgctagc ccacgcaatg
     1021 tgcgattgac ggcctgtttt ccgattaatt gcgtcgcatg actgtcaaag atcgttaatt
     1081 gccgttttgt aaatggccgg aaatttttgt ctttagatac gcagtccaaa ctattatttt
     1141 tgatctggaa tcgtggactc cttcatcgca tgaatcgaag gactgtagac caccttttta
     1201 tctggagtga ggttcggcgc aaaagcgtag accgtgacgc gaacaagcgt ttcgtatcag
     1261 tgtctgtccg cacacaaaag agcatttgca aaggcggtag tgaggaccat cacacagaca
     1321 gtgcacgcgc gctctcccgt ggttttctcg ttacgcacat cgaccaccgc ctgcgcatcc
     1381 gcgctatcgt gatccatgcg tcgcggcacc gaccaggaaa gcgcgcgaaa accgaatcag
     1441 gcgacaatag gaaaatcgca acctcatcaa caacgggaac agagcgatgc ttttatccga
     1501 acaatgggtg ctggtaacgg gtggcgcccg cggactcggc gcggccatca cgcgtgcact
     1561 gttacgcgaa ggcgcgggcg tcgtggtgaa ttaccgcagg agcgaaacgc tggcccacgc
     1621 gctaagagaa gagtttggcg accgtgtcct gccgatccag gcggacgtga tcgacgcgac
     1681 cgccattgcg cgtctattcg aaaccgcgca ggcacaaacc gggcaaccca tcgtctcagt
     1741 ggtcaacaac gcgctggccg actttcagtt cgacggcgac gcccgcccga aactcgactc
     1801 gatcgactgg gcgcgcttcc agcagcagtt ggacggttcc ctcaagggcg cgctcaatac
     1861 gatgcaagca gcagccgcag gcatgcgctc gcgcggcttc ggccgcatcg tgaacgtcgg
     1921 cacgaatctg tttcagaatc ccgtggtccc ctatcacgac tacacggcgg ccaaagcggc
     1981 gctgttgtcg ctcacgcgca ccgcggctca cgatctcggc ccggacggga tcacggtgaa
     2041 catggtgtcg ggcgggctgc tacgcaccac ggacgcgagc gcggctacgc cggaattcgt
     2101 cttcgacctg atcgccagtt cgacgccgat gcggcgcgtc acgacccctg aagagttcgc
     2161 cgacgccgta ctgttcttcc tgtcgccgtg gtcgcgcgcg gtcaccggtc aaaatctgat
     2221 tgtggacggt ggcctcgtga agggttgatg ctatggaagt gtcgccgcgc agccaccacc
     2281 ccggcgccta accaacactt cttcgcggca acttttcccg aagcgtttcc accatccgga
     2341 gcctcgcgga gggtcatcat tctccctgcg acacgcagac acttgcggca cgaggtttcg
     2401 cgcgatatcg gccgcgggtt cgggtttacc ttcgtttgac ttccggaggc aggcccgttt
     2461 actggacgca aatcaaacct ttcagacgcc ttcattttgc tttgttcagc tgaatagtgt
     2521 tctgaaagcg tctctactgt gtctggaggt gtgtcatggt gagtgtgtcc agcagtgccg
     2581 tcgaagaact caaagccgcg gtccgaggcc aactgctgcg gcccggcgac gcgggtttcg
     2641 acgaggcccg cagtatctgg aatgcaatga tcgaccggca tccggcaatg gtattgcgct
     2701 gcgccggcgc ggccgatgtg cgccgcggcg tagcgttcgc gcgcgacaac ggtctgccgc
     2761 tggcggtgcg cggcggcggc cacaacatcg ccggtaccgc gctgtgcgac gatggccttg
     2821 tgatcgacct gtcgctgatg aaatcagtac atatcgatgc ggcagcgcgg cgcgcgtatg
     2881 tcgagccggg cgcgacgctc cgcgatttcg accacgaagc gcaagcgttc ggactcgcca
     2941 ccccgctcgg catcaactcg accaccggcg tggcgggtct gacgctcggt ggcggcttcg
     3001 gctggctgag ccgcaaatac ggcatgaccg tcgacaatct gatttcagcg gacgtcgtca
     3061 ccgccgacgg cgagctggtc cacgcgagcg ccgagtcaca cccagatctg ttctgggcga
     3121 ttcgcggcgg cggcggcaat ttcggcgtcg tgacgcgttt cgaattcgcg ctgcatgcgg
     3181 tcgggccact ggtctacggc ggccttgtgg tattgccgat cgaacaggca aagaatgctc
     3241 tgctccagta ccgcacggcg gcagaacaga tgccggacga tctgagcgta tgggtcgttt
     3301 cgcggcttgc cccgccgctg ccgttcctgc cgcccgaagc ccacggcaag ccggtcatcg
     3361 tatttgcaat gtgctacacc ggcccggtcg aaaacgggcc cgctgtggtg caggccgtgc
     3421 gcggcttcgg cacacccatt ggcgaacatc ttgggccgat ggcctacgcc gcatggcaac
     3481 aggcattcga tccgctgctc acgccaggcg cgcgcaacta ctggaagtca cacaatatag
     3541 gcaagatttc ggacggtctc atcgatgccc tgctcgatgc gatcggcaaa ctaccgtcgc
     3601 cgcaatgcga aatcttcttc ggccagatcg gcgcacagac tagccgggtg cctgtcgaag
     3661 cgacggccta ttcgagccgc gacacgcagt acgcgatgaa cgtgcacggc cggtgggacg
     3721 acgcgagcga tgacgagcgc tgcattggct gggcccgcgc attcttcgac gcatccgcac
     3781 cgttcgcgct tggcagcgtg tacgtcaact tcatgacaca ggaggaaagc ggccgggtgg
     3841 ctgacgcgta tggaccgaac tacgagcgtc tcgtcgccgt gaaaaagcac tacgacccgc
     3901 acaacctgtt ccgccacaat cagaatatcc gcccggcggc gtagggactg cggaggagcg
     3961 gagctgaacg tcgtcagtcc gcgcgcgaac gtcagctcag cgcggctgtc tgatacacaa
     4021 cccgtggcgt caggcacgtt aaatgctgct tggcaattga cgtacgagct tgctggcgtc
     4081 acggcactta cggttcagac agcaaccaca acctccatca cgcacagggg aaataatcat
     4141 gcgcagacga caattcatca tgaccacgag cgccgcgttg gcaactgcgg gttttagcct
     4201 cgccggctgc accaccacct cgccgtcctc gagcgcgtcg ccttcggcga acgccggcaa
     4261 gcgcgacacg atcaatgccg gtgtcgattc cacgctctct cgcctgtacg cgaacgtcgc
     4321 cggatcgcgt gaactggtgg gcaaggcgcg cggcgtgctc gtgttcccgt ccgtgatttc
     4381 ggcgggcttc tgggtcggcg gccaatacgg cgaaggcgcg ctgcgcgtgg ccggacgctc
     4441 gtcgggctac tacagcaccg tcgcgggctc gttcggtttg cagatcggcg cacagtccaa
     4501 ggcgctggtg tttctgttca tgacacaaga ggcgctcgac agattcctcg gcagtcaggg
     4561 atgggcagca ggcgccgatg ccacggtcgc cctgttgaag atcggcgcga acggcgcggt
     4621 cgatacctcg accgctacca gccccgtcga agcttttgtg ctgacgaacg gcggcctgat
     4681 ggccggtgta tcgctcgaag gcaccaaggt ctcgcgtctg atcatctgag agaagcgcgc
     4741 gccgaatgcg ttcgtggtgt ttttggtgta ttgcgtatgg cacgccgatg ccgtcgctga
     4801 cacccacaat caggcgcagt cacgcggtcg tgcaagcggc tgcgtgacat cccgtgtgac
     4861 ttctccatcc ggcggcgtcg gttgcgccgc gatcagcgct ctgactgccc cgccggtcac
     4921 tttgaccgca ctgctgtaaa attcgcccgt tttgcaatcc tgcctggcca ctgctccgcc
     4981 ttagccacga cgttcttcct gtctggcgcc tcgcgcatcg ccacgctgtt tgcccctcgg
     5041 tttcgccact cctccactga ctgcgcgcgt aaggctttcg cgtgcggaaa gcgacggcat
     5101 gccgactttt acgcatcgat agcgcagccg cacagccgac gcgctctcaa tcccaagtcc
     5161 ccgttccctt cagatgaaca ctgctagtcc cactccccgt tggctcgcca aacttccagg
     5221 tatcaagagc aatgtgctcg cgggcctcac ctcgtcgttc gcgttggtgc cggaatgtat
     5281 cgccttcgcg ctggtcgccc acctcaatcc cttgatgggt ttgtatggcg cgttcttcat
     5341 ctgcacgatc acggctttgt ttggcgggcg gcccggcatg atctccggcg cggccggttc
     5401 gatggccgtg gtgatcgtgg cgctggtcgt gcaacacggc gcgcaatatc tgctcgccac
     5461 cgtgatcctc agcggcatcc tgatggtgct gttcggcgcg ttgcggctgg gcaagctgat
     5521 tcgcatggtg ccgcacccgg tgatgctcgg tttcgtcaac ggcctcgcga tcatcatcgc
     5581 gatggcgcaa ctggcgcact tcaggcaaag cacgccgcaa ggcgaacaat ggctgcacgg
     5641 cagcgcgttg ctgttgatgt gcggtctcgt cgcgctaacc atgctgatcg tctatctgct
     5701 gccacggctc acacgcgctg cgccgcccgc actggtggcg atcgtcggcg tggggctctt
     5761 cagtcagctg tcgcacctgc ctgcgcgcac gctcggcgac atggcccaca ttgccggtgg
     5821 tctgccgagc ctccatgtgc ccagggtgcc gctgaatctc gacacgctgc atgtggtgct
     5881 gccttacgcg gttctgatgg cgatcgtcgg tctgctggaa acgctgctta cgttcaacct
     5941 gaccgacgag atcaccgaca cccgcggcca gcctaatcgc gaatgtctcg cgctgggcgc
     6001 ggcgaatatc gcctcgggcc tgttcggcgg catgggcggc tgcgcgatga tcgggcaaac
     6061 catgatcaat ctgaattcgg gcggccgttc gcggctgtcg ggcatcgtca gcggcgtgat
     6121 gatcctgatg ttcatcctgt ttctgtcgcc cctgatcgag cggattccac tggcggcgct
     6181 ggtcggcgtg atgttcgtgg tggcgcagca gaccttcgca tggggttcgc tgcgggtatt
     6241 gggcaaggtg ccgcgcaacg acgcgctggt catcgttgcg gtcacaacca tcaccgtgtt
     6301 ctccgatctg gcgatcgcgg tgttgtgcgg catcgtgatc gccgcgctca atttcgcctg
     6361 gcaacacgcc cgtgaaattc acgcgcatgt tgaagatcac ggcggcgaca aaacctacgt
     6421 tccgcgcggc acgctgtttt ttgcgtcgac cagccgcttt cacgagcttt tcgatccggc
     6481 aaaagatccc ggccacatca cagttgattg caggcacttg acgctcgcgg atcattccgc
     6541 attggccgcg ctgcacggac tgttcgaacg ctatcgaaaa gccggcaagc aattgcgtgt
     6601 ggagaatctg tcggagcgca accagcgcct gctgagccgc gccggcattg agatttcggg
     6661 acgcggcgcg gcttgacgcg ccccgtagtc ggcccagatt gctcgtcccg gtccgccacg
     6721 tcccctgtaa tatatgaaca ttgacgaaag gtaaatcgac ccgaagccat gtctttttta
     6781 ctacattaga agattcgtgc agcctgccgg agggcccgtg acaagacgcg tttcgctcga
     6841 ggatgaat
//
LOCUS       DATASH010001031        10568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5319744,
            whole genome shotgun sequence.
ACCESSION   DATASH010001031 DATASH010000000
VERSION     DATASH010001031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 10568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10568
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5319744"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            377..799
                     /locus_tag="VHV99_27390"
     CDS             377..799
                     /locus_tag="VHV99_27390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX3639142.1"
                     /translation="MNRPLSYDECNCFALRQAARHVTQIYERHLGGVGLTAAQFTILA
                     KLARTPNLPMAELADAMVMERTTLVRAMKPLQRDGLVVAEPAEHDGRTFLFSLTEKGE
                     TTFDQAAIAWRAAQDEFEEKFGHARAKMLRAELFSITG"
     gene            881..1303
                     /locus_tag="VHV99_27395"
     CDS             881..1303
                     /locus_tag="VHV99_27395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018434136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX3639143.1"
                     /translation="MDGLFDDDCFAIRQAARYVSQLYDRHLENVGLTITQFSLMGRLK
                     RTGPMTMKQLAEAMRMQRTTLVRTIQPLRRNGLVSSETLGADARALSIALTAGGIERL
                     QAGREHWYAAQAEFENRFGEQRAAALRAELFAITNDPV"
     gene            1393..2547
                     /locus_tag="VHV99_27400"
     CDS             1393..2547
                     /locus_tag="VHV99_27400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006046552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEX3639144.1"
                     /translation="MSTTPSTIAPPSVASAAKPARRIPWMLLAIITVLAVLALAASYW
                     FFVGRFVESTDDAYVGGDVTVMSPKVSGFVTDVLVRDNQFVHANQVLIRLDARDYDAR
                     LAQATAEVQSAQAAVTELQAKKSLQLATINEQAAEVRASGAELTRSAADQTRYRELVK
                     DEAVSNQVVERADADLTKAHAAVDRSNAGLIAAQRQIAVLDAQIGDAEARIATAQAAQ
                     RVAALNVEYTTIRSPIDGYVGNRTARVGLLANTGVSLLTVVPSSGLWIDANFKEDQLK
                     KMHVGDGVDVDLDASSKQIHGVVESLAPATGATFSVLPAENATGNFTKIVQRVPVRVR
                     LDVPKDMQGVLRPGLSATVKVHVDAPQSPKETAVGAENSPRGAGNAPARG"
     gene            2555..4135
                     /locus_tag="VHV99_27405"
     CDS             2555..4135
                     /locus_tag="VHV99_27405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009771628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX3639145.1"
                     /translation="MTQAPAHIANPADQPTRTKFFAFALMCLGFFMATLDIQIVASSL
                     RDIGGGLSASQDELSWVQTAYLIAEILVIPMSGWLTRVFSTRWLFAFSALGFTITSML
                     CGLAWDINSMILFRGLQGALGAAMIPTVFTTAFVLFPGRQRLIAATTIGALATLAPTI
                     GPVIGGWITAQWSWHWLFYLNLAPGVAVTVLVPKYVHIDEVDLSLLKKGDYLGILLMS
                     GFLGCLEYVLEEGPRKNWFGDDVILLCTWVSAICGFLFIVHAFTAKEPIVDLRALAIR
                     NFGIGSLLSFITGIGIFCTVYLTPVFLARVRGFDSLQIGLALLSVGCFQLVSLVAYST
                     LARFVDMRKLMVFGLILFGIGCYLYVPLTSQWGWKELLLPQALRGIGQQFCIPPIVTM
                     ALGSLPPSRLRSASGLFNLMRNLGGAIGIAVSTTMLNDRLNLHYERLDEHLSAGRPAV
                     ESILQQQSAHFAAIGGNALTAANAGLGELHALLMREALVLTFSDTFFALSLCFVVGLL
                     SVLFSRPFGNTAPPPDAH"
     gene            4146..5576
                     /locus_tag="VHV99_27410"
     CDS             4146..5576
                     /locus_tag="VHV99_27410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006046550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEX3639146.1"
                     /translation="MQPIVIKALASAALLTLAACAVQPATHADLPHTVQSVAPSAWNV
                     DAPQDSVSANTWWAQFGDPVMHQLVESVLTGNLDVQAAVERVKQAQDLATQNRAALLP
                     ELNASAGASDSRQNTPPPLGYVRQAGIGVTASWTPDVFGGERLAVLAAQAQVSGRQAA
                     LNQLRLALAANTAAAYIDLRWAQSQLQILSDNEQIRARALKLTQQRLHYGLSTQLDVA
                     RAQNQLEDLQAQIPRMQSAVQHQLSLIAVYSGRTPESVDGLLLANARDIPVPAQSVPQ
                     TLPSDALLQRPDVRTAYATVEQRAAEVGVSKAQRYPQFRLDLADGLLASSYLGLPTLT
                     DNLFSAALNATSPIFNAGRITANIDASESRMRESQLGLQQTMLQALKEIEDNRSDLVN
                     GEVQVQHLGGALDASDHALRLSTELYKGGASSFLDVLDAQEAYLRDAESLNQARREHA
                     LAAVALYRSLGGGWDVPDAVTAASAN"
     gene            5625..6809
                     /locus_tag="VHV99_27415"
     CDS             5625..6809
                     /locus_tag="VHV99_27415"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011881353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX3639147.1"
                     /translation="MNTREVVICNPVRTPIGAFGGSLKDVPATELGAVAVRETLRRSG
                     LDPAALASVVLGNVIQAGNKMNPARQASIGGGVPVAVPALTVNRVCGSGAQAIASAAQ
                     EIWLGLGDAAVAGGMENMDRAPYLLDGGRWGYRMGNAQIHDSLLRDGLNDAFSGEHSG
                     WHTEDLVAQFDLTRETQDRWAARSQQRFTEAQARGDFDAERVAVEIQGRKGPQHFTSD
                     EQPRPDTTIETLARLRPAFRPDGTITAGNAPGLNSGAAAMLVAERGFAEAHGIEPFAR
                     LVAYGVAAVEPGMFGLGPVPAVQMALARAGWQLDDVERFEINEAFAAVPIAVARRLGI
                     ADELINVQGGAIAHGHPIGATGAVLTTRLLHSMRRDGLKRGIVTLCIGGGQGIALALE
                     VI"
     gene            7056..7445
                     /locus_tag="VHV99_27420"
     CDS             7056..7445
                     /locus_tag="VHV99_27420"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEX3639148.1"
                     /translation="MNMEQLLERAGVAREKAYVPYSKFKVGAALLTKDGKVFDGCNVE
                     NASYGLCNCAERTAFFSAIAAGYQRDQFAALAVIGDSDGPIAPCGACRQVIIELGGPE
                     LPIRLGNLRGATRDTTAREQLPDAFYL"
     gene            7442..8419
                     /locus_tag="VHV99_27425"
     CDS             7442..8419
                     /locus_tag="VHV99_27425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEX3639149.1"
                     /translation="MSRHKVIYDTDPGVDDAMALVFQALHPDIELLGLTSVFGNATIE
                     TTTRNARFLAGRFAPGVPVAQGAAAPLKRAVPEPFALIHGDNGLGNIAIEAERDAGLE
                     AALDGRPAHRFIIDTVRAHPGEVTLIAVGPLTNLALALADDPRVATLVKQVVIMGGAF
                     GTDGVLGNVTPAAEANILGDPDAADIVLGAAWPVAIVGLDVTQRTIMSQDYLASLRDR
                     GGAAGRFVWDVSRHYEDFHQQSAQLKGIYVHDSSAVTYVLAPHLYTVRSGPVREETEG
                     IAVGQTIQKPSTMPVPAPDWDNRPECEVCVAVDVPGMLALYERTLCGTP"
     gene            8743..9678
                     /gene="panE"
                     /locus_tag="VHV99_27430"
     CDS             8743..9678
                     /gene="panE"
                     /locus_tag="VHV99_27430"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX3639150.1"
                     /translation="MRILVVGAGAVGGYFGGRLAAAGQDVTFLVRPGRAEKLQRDGLV
                     INSARGDLTLRDVKTILAGVGAEPFDLVLLSCKAYSLDDAIDSFAPLVGESTLILPML
                     NGMRHIDVLKEKFGASRVLGGQCVIAATLSPEQHIMHLNEMHAITFGELEGGTSERVE
                     AIADAMAGANFDALVSDNILLRMWEKWVFLATLAASTCLMRGSVGDILAAPDGKRIIE
                     NLLGECRGVAERNGFTMGQDFDARATQTLFTPSPLTASMLRDVENHSHTEADHILGDL
                     ISRGGDAQKGEHGLSLLRIAYSHLKTYEARQTRTS"
     gene            9737..10036
                     /locus_tag="VHV99_27435"
     CDS             9737..10036
                     /locus_tag="VHV99_27435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639151.1"
                     /translation="MPSPSGSVPALSSASATIFSIGIVFLGYWGLYDQTKWRVSDVFV
                     FVFAMIGFGCLGLVPWLATSPVEAETNDSRIRIARHMFLAGVLSIWLGVAISVIF"
     gene            complement(10037..10183)
                     /locus_tag="VHV99_27440"
                     /pseudo
     CDS             complement(10037..10183)
                     /locus_tag="VHV99_27440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011548368.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            10172..10291
                     /locus_tag="VHV99_27445"
                     /pseudo
     CDS             10172..10291
                     /locus_tag="VHV99_27445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006479663.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
     gene            complement(10306..>10568)
                     /locus_tag="VHV99_27450"
     CDS             complement(10306..>10568)
                     /locus_tag="VHV99_27450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639152.1"
                     /translation="KDHARVIFKGASRYLVNDFLMQNVIYSMDVLTDFESDEYKRALD
                     ALDNSYSWGRNKPRKNIAAITATFGAELFIEFDSLAVESIAR"
BASE COUNT         1900 a         3235 c         3375 g         2058 t
ORIGIN      
        1 acccgcaggc tttccgactg cccgtgtgac ggtcggcttc cagatgaccg cgcagttctg
       61 aagttcataa gtcgcgcccc gcctcggtca acgttaacca accaagcgag ctaaacgcgc
      121 gaactataac taatttttac cttcaccccg gcacgggcct ctgataatcg tcacacagca
      181 gaccgtgcca ccaaacaaac cggctcgccg aattactcct gcgagccgtt cttcacttcc
      241 agcgcactgc atacctactg cgcaaacgcc ccaatagcca ggaacggatt gaccttactg
      301 gcgttattca ccggatcgtg gtcttggaac gcaagtaaac ttgactatgt gcgtatgcac
      361 atatacactg cccatcatga accgtcctct ctcttacgac gaatgcaact gcttcgcgct
      421 gcgccaggcg gcgcggcacg ttacgcagat ctacgagcgc catctcggcg gggtgggtct
      481 gacagccgcg caattcacga ttctcgccaa gctcgcgcgt acgccgaacc tgcccatggc
      541 ggagcttgcg gacgccatgg tcatggagcg caccacgctg gttcgcgcca tgaaaccgtt
      601 gcagcgcgac ggcctggtgg tagccgagcc ggcggagcat gacggccgca cgtttctgtt
      661 cagtctcacg gagaagggcg aaacgacctt cgaccaggcc gccattgcat ggcgtgccgc
      721 gcaagacgag ttcgaagaaa agttcggcca cgcgcgggcc aagatgctgc gcgccgaact
      781 cttcagcatc accgggtagc caaagcgtac gagctcgttc cgacgagctt cggtacgcct
      841 gattgtgtgc atacgcactc acagaaaagg aagtgcagcg atggacggac tcttcgacga
      901 cgactgcttt gcgattcgtc aggccgcacg ctacgtgtcg cagctttacg accgtcacct
      961 tgagaatgtc ggcctcacca tcacgcagtt ttcgctgatg ggccgattga agcgcaccgg
     1021 tccgatgacc atgaagcaac tggccgaagc catgcgcatg cagcgcacca cgctggttcg
     1081 cacgatccag ccgctgcggc gcaacggact cgtgtcgagc gagacactcg gggcggacgc
     1141 gcgggcattg tcgattgcac tgacggccgg gggcattgag cgtttgcagg caggacgcga
     1201 gcattggtat gcggcgcaag ccgaattcga gaatcgcttc ggcgaacagc gcgcggcggc
     1261 attgcgcgcc gagttgttcg ccatcacaaa cgatccggtt tgaatcaggc ccgcatgaag
     1321 cgggtcaagc ccggcgcagc gtaacgcgcc gtttttttca gctcaacgag tgcatacaca
     1381 cattcgaaca ccatgtccac taccccttcg actattgcac cgcccagcgt ggcaagcgcc
     1441 gccaaaccgg cccggcgcat tccgtggatg ctgcttgcca tcatcacggt gctcgccgtg
     1501 ctggcgcttg ctgcgtcgta ctggttcttc gtcggccgct ttgtcgaatc gaccgacgat
     1561 gcatacgtgg gcggcgatgt caccgtgatg tcgccgaagg tgagcggctt cgtcacggac
     1621 gtgctggtgc gcgacaacca gttcgtccat gcgaatcagg tgctgatccg gctcgacgcg
     1681 cgcgattacg atgcccgcct cgcgcaggcc actgcggaag tgcagagtgc acaggccgcc
     1741 gtcaccgagt tgcaggcgaa gaaatcgctg cagctcgcca ccatcaacga gcaggccgcc
     1801 gaggtgcgcg cgtccggcgc cgaactgacc cgcagtgccg ccgatcagac gcgttatcgc
     1861 gaactggtga aggacgaggc ggtatcgaat caggttgtcg aacgcgcgga cgccgatctg
     1921 accaaggccc atgccgcagt tgatcgcagc aacgccgggt tgattgcggc gcaacggcag
     1981 attgccgtgc tcgacgcgca gatcggtgac gccgaggcac gcatcgccac cgcgcaggcg
     2041 gcgcagcggg tcgcggcatt gaacgtggaa tacacgacga tccgctcgcc gatcgacggt
     2101 tacgtcggca atcgcaccgc acgcgtcggc ctgctggcga acaccggcgt ttcgctcttg
     2161 accgtggtgc cgtcgagcgg actgtggatc gacgccaact tcaaggaaga ccagctgaag
     2221 aagatgcacg tcggcgatgg tgtcgacgtc gatctcgatg cgtcgagcaa gcagattcac
     2281 ggcgtggtgg agagtctcgc ccccgcgacc ggtgcgacgt tcagcgtgct gccggcggaa
     2341 aatgccaccg gcaacttcac gaagatcgtg cagcgcgtgc cggtgcgtgt gcgtctcgac
     2401 gtgccgaagg acatgcaagg cgtgctgcgc cccggtcttt cggcaaccgt gaaggtgcat
     2461 gtcgacgccc cccaatcccc caaggagacc gccgtcgggg cggagaactc ccctcggggc
     2521 gccggcaatg cgccggcccg cggatgagcc gatcatgact caggcccccg ctcatatcgc
     2581 caatccggcc gatcagccga cgcgcaccaa gttcttcgcg ttcgcgctga tgtgcctcgg
     2641 ctttttcatg gcgacgctgg acatccagat cgttgcgtcc tcgcttaggg acatcggcgg
     2701 cggtttgtcc gcgagccagg acgagctctc gtgggtacag accgcctatc tgatcgccga
     2761 aattctggtg attccaatgt cgggctggct cacgcgtgtt ttttccacgc gctggctgtt
     2821 tgcgttctcc gccctcggct tcacgatcac cagcatgttg tgcggtctcg catgggacat
     2881 caactcgatg atcctgtttc gcggtctgca gggcgcgctc ggtgcggcga tgattccgac
     2941 cgttttcacc acggcgttcg tgctgttccc cggcagacaa cggctgattg cggccactac
     3001 gatcggcgcg ctggccacgc tcgcaccgac catcggtccg gtgataggcg gctggatcac
     3061 ggcgcaatgg tcgtggcatt ggctgttcta tctgaacctc gcgccgggcg tcgccgtcac
     3121 ggtgctggtg ccgaaatacg ttcacatcga cgaggtcgat ctttcgctgc tgaaaaaagg
     3181 cgactatctc ggcatcctgc tgatgtccgg ttttctcggc tgcctcgaat atgtgctgga
     3241 ggaaggcccg cgcaagaact ggttcggcga cgacgtgatc ttgctatgca catgggtgtc
     3301 ggcgatctgc ggtttcctgt tcatcgtgca tgcgttcacc gcgaaagaac cgatcgtcga
     3361 tttgcgcgcg ctggcgatcc gcaacttcgg catcggcagc ttgctgtcgt tcattaccgg
     3421 gatcgggatt ttctgcacgg tctatttgac gccggtgttt ctcgcgcgcg tgcgaggctt
     3481 cgattcgttg cagatcggct tggcgttgct gtcggttggc tgctttcagt tggtctcgct
     3541 ggtggcgtac tcgaccttgg ccaggttcgt cgatatgcgc aagctgatgg tgtttggact
     3601 cattctattt ggtatcgggt gttatctcta cgtgccgttg accagtcagt ggggctggaa
     3661 agaactgctg ctgccacagg cattgcgcgg catcggccag cagttctgta ttccacctat
     3721 cgtgacgatg gcgctcggct ccctgccacc ttcgcggctg cgctcggcaa gcggcctgtt
     3781 caatctgatg cgcaacctcg gcggcgcgat cggcattgcg gtgagtacca cgatgctcaa
     3841 cgatcgattg aatctccact acgagaggct cgacgaacat ctgagcgcgg gacgtccggc
     3901 ggtcgaatcg attttgcagc agcaaagcgc gcacttcgcg gcgataggcg gcaatgcgct
     3961 gactgcggcc aatgcgggcc tcggcgagtt gcatgcgctg ctgatgcgcg aagcgctcgt
     4021 gctcacgttc tccgacacgt tttttgcgct gtcgctctgt ttcgtcgttg gtttgctgag
     4081 cgtgctgttc tcgcgcccat tcggcaatac cgcgccgccg cccgatgctc attgaggaat
     4141 tcagcgtgca acctatcgtt atcaaagcac tggcgagcgc ggcattgctg acgcttgcgg
     4201 catgcgcggt gcagcccgca acgcatgcgg acctgccgca tacggttcag agcgtcgcgc
     4261 cgagcgcatg gaatgtcgac gcgccgcaag acagtgtgag cgccaacacg tggtgggcgc
     4321 aattcggcga ccctgtcatg catcaattgg tcgagtcggt actgaccggc aatctcgacg
     4381 tgcaggccgc cgtggaacgc gtcaagcagg cacaggatct cgcgacgcag aatcgcgcgg
     4441 cgttgctgcc cgagttgaac gccagcgcgg gcgcatccga ctcgcgtcag aacaccccgc
     4501 cgccgctcgg ctatgtgcgc caggccggca tcggcgtgac ggcgagctgg acgccggacg
     4561 tcttcggcgg tgaacgtctt gccgtgctgg cggcgcaggc gcaggtgtcg ggccgccagg
     4621 cggccttgaa ccaactgcgt ctggcgctcg cggcgaacac ggcggcggcg tatatcgatc
     4681 tgcgttgggc gcaatcgcaa ttgcagattc tcagcgacaa cgaacagatc cgcgcccggg
     4741 ccctaaagct cacgcaacag cgtttgcatt acggactgtc gacgcaactc gacgtcgcgc
     4801 gagcgcagaa ccagctggaa gatttgcagg cgcagattcc gcgcatgcag tcggcagtgc
     4861 agcatcaatt gagtctgatc gccgtgtatt cggggcgcac gccggagagc gtcgacggtt
     4921 tgctgctggc caatgcacgc gacattccgg tgcccgcgca aagcgtgccg cagacgctgc
     4981 cgtccgacgc gctgttgcag cggcccgacg tgcgcacggc ctacgcgaca gtggaacagc
     5041 gcgcggcgga agtgggcgtg tcgaaggcac agcgctatcc gcagttcaga ctcgatctcg
     5101 ccgacggctt gctggcttcg tcctacctcg gcttgccgac gctgaccgat aacctcttta
     5161 gcgccgcgtt gaatgcaacg agtccgatct tcaacgccgg ccgcatcacg gcgaacatcg
     5221 acgcgagcga gagccggatg cgcgagtcgc aacttggatt gcagcaaacc atgctgcagg
     5281 cgctcaagga aatcgaggac aaccgcagcg atctggtgaa tggcgaggtg caggtgcaac
     5341 acctgggcgg cgcgctcgat gcatcggacc atgcgctgcg tctgtcgacg gagctttaca
     5401 agggcggcgc gtcgagtttc ctcgatgtac tcgacgcgca ggaggcgtat ctgcgcgatg
     5461 cggaatcatt gaatcaggcc aggcgcgagc atgcgctcgc ggccgtggcg ctataccgct
     5521 cgctgggtgg cggctgggat gtgcctgacg cggtcacggc ggcatcggcg aattgatgcg
     5581 cgtgcacgtg gcgatcaatg gggtagtcca actggagatg aacgatgaat acgagagaag
     5641 tagtgatctg caatccggtg agaacgccga tcggtgcgtt tggcggatcg ctgaaagatg
     5701 tgcccgcaac cgaactcggc gcggtcgcgg tacgtgagac gctgcgtcgc agcggactcg
     5761 atccggctgc gttggcctcg gtggtgctgg gcaacgtgat tcaggccggc aacaagatga
     5821 atccggcgcg tcaggcgtcg attggcggcg gtgtgccggt tgccgtgcct gcattgacgg
     5881 tcaaccgtgt atgcggttcc ggcgctcagg caattgcgtc cgcagcccag gaaatctggc
     5941 tcggcctcgg cgatgcagcc gtggccggcg gcatggaaaa catggaccgt gcaccgtatc
     6001 tgctcgacgg cggccgctgg ggttaccgca tgggcaacgc gcaaatccac gacagtctgc
     6061 tgcgcgacgg cctgaacgac gcgttttccg gcgagcattc cggctggcat accgaagatc
     6121 tcgtcgcaca gttcgacctc acgcgcgaaa cgcaggaccg ctgggccgcg cgttcgcaac
     6181 agcgttttac cgaggcgcag gcgcgcggtg atttcgacgc tgaacgggtc gccgtggaaa
     6241 ttcaggggcg caaaggtccg cagcatttta cgagcgatga gcagccgcgc ccggatacca
     6301 ccatcgaaac gctcgccaga ttgcgtccgg cgttccgccc ggacggcacg atcaccgcgg
     6361 gcaacgcgcc gggattgaac agcggcgcgg cggcgatgct ggtcgccgag cgtggcttcg
     6421 ccgaggcgca tggcatcgag ccattcgcgc ggttggtggc gtatggcgtc gcggccgtcg
     6481 agccgggcat gttcggcctc ggtccggtgc cggctgtgca aatggctttg gcccgtgccg
     6541 gttggcaact ggacgacgtc gaacgcttcg agatcaatga agccttcgct gctgtgccca
     6601 ttgcggtggc gcgcagactc ggcatcgccg atgagctgat caacgtgcaa ggcggcgcga
     6661 ttgcacacgg tcatcctatt ggcgcgaccg gcgcggtgtt gacgacgcgc ctcctgcatt
     6721 cgatgcgccg tgacggcttg aaacgcggca tcgtcacgtt atgcatcggc ggtgggcagg
     6781 ggattgcgct ggcattggaa gtgatctgaa agcggcggcg gcgcgttgtc gtcctgaaac
     6841 gctaacgcgc ccgccgggtt attcgttgtc ttttagatgc gcggcgcgtg agcttcgatc
     6901 ccgttgctca gcaaacacgc gccgcccgct atttaaaggt tttcctacgg cacaagtttc
     6961 gtttagcatc attgccactc gttgtggaac ctgatgagcg ccacgattgc cgccatgttc
     7021 ctgacgctga attgaattga agcgaggatc gttgcatgaa catggaacaa ctgctggaac
     7081 gcgcgggtgt tgcgcgagaa aaggcttatg tgccttattc gaaattcaag gtcggcgccg
     7141 cgctgctgac caaagacggc aaagtattcg acggttgcaa cgtcgagaat gcctcgtacg
     7201 ggctgtgcaa ttgcgcggaa cgtacggcgt ttttcagcgc tattgccgcg ggctatcagc
     7261 gcgaccagtt cgccgcgctt gccgtgatcg gcgattcgga tggaccgatc gcaccgtgcg
     7321 gcgcatgccg ccaggtgatc atcgaactgg gcggaccgga attgccgatc cgcctcggca
     7381 atttacgcgg cgcgacgcgc gacaccaccg cgcgcgaaca attgccggac gcgttctatc
     7441 tatgagcagg cacaaggtta tctacgacac cgaccccggt gtggacgatg caatggcgct
     7501 cgtttttcag gcgctgcacc cggacatcga gttgctcggt ctgacgagcg tattcggcaa
     7561 cgccacgatc gaaaccacca cgcgcaatgc gcgtttcctg gccggacggt tcgcgccggg
     7621 tgtgccggtt gcgcaaggcg ccgctgcgcc gctcaaacgc gcggtgcccg agccgttcgc
     7681 cttgattcac ggcgataacg gacttggcaa tatcgcgatc gaggccgagc gtgacgccgg
     7741 tcttgaagca gcgctcgacg gccgtcccgc gcaccgcttc atcatcgaca cggtgcgcgc
     7801 gcatcccggc gaagtcacgt tgatcgccgt gggaccgctg acgaacctgg cattggcgct
     7861 cgctgacgat ccgcgggtcg ctacgctcgt caagcaggtg gtgatcatgg gcggcgcgtt
     7921 cggcacggac ggcgtgctcg gcaacgtgac gcccgccgcg gaagcgaaca ttctcggtga
     7981 ccccgatgcg gccgatatcg tgctcggcgc ggcgtggccg gtggcgatcg tggggctcga
     8041 cgtcacgcag cgcaccatca tgagccagga ctatctggcc tcgctgcgcg atcgcggcgg
     8101 tgcggcgggg cggttcgtgt gggacgtgtc gcggcactac gaggactttc atcagcaaag
     8161 cgcacaactc aagggtatct acgtacacga ctcgtcggcg gtgacctatg tattggcgcc
     8221 gcatctttat acggttcgca gcggtcccgt gcgcgaggag acggagggta tcgcggtggg
     8281 ccagactatc cagaagcctt ctacgatgcc ggtgcccgcg ccggattggg acaaccggcc
     8341 tgagtgcgaa gtttgcgtgg ctgtggacgt gcccgggatg ctcgcgcttt acgagcgcac
     8401 cctctgcggc acgccgtagg ccgatcttgc aacgctgtcg ccgctttgcc cggcgagcct
     8461 tcgcgcatcg attcctcgcg gtgcctgctc gccgtgcgtc cttttacgta cagggcgagc
     8521 cgtgtggacg gccggcggcg cggcgattga tctgacataa cgacagtccg gaacgatgtg
     8581 gaacaccgtg gacgcggtgc gttgcaacaa aaggccggca tttgtggccg atgcccatcg
     8641 tcaatctttg ctacatttct ccccacgtgc tgtatgcgcg gtccttaagg cgttggctta
     8701 tcgccccggc gcgcgtgact caaatctatc gagaggcaaa agatgcgaat tctagtagta
     8761 ggggcgggcg ccgtaggcgg ctacttcggc ggacgtctcg cggcagcggg ccaggacgtg
     8821 acctttctgg tgcgccccgg ccgtgcggaa aaactccagc gtgacggcct cgtcatcaat
     8881 agcgcgcgcg gcgatctcac gttgcgcgac gtcaagacga ttctcgccgg cgtcggcgcg
     8941 gagccgttcg atctggtgct gctcagttgc aaagcctaca gtctcgacga cgcgatcgat
     9001 tcgttcgcac cgctggtcgg cgaatcgacg ctgattctgc caatgctcaa cggcatgcgc
     9061 catatcgacg tgctcaagga aaagttcggt gcgtcgcgcg tactgggcgg tcaatgcgtg
     9121 atcgcagcga cgctcagccc cgagcagcac atcatgcatc tgaacgaaat gcatgcgatc
     9181 accttcggcg aattggaagg cggcacgtcg gagcgcgtag aggcaatcgc ggacgcaatg
     9241 gccggcgcga atttcgacgc gctggtcagc gacaacatcc tgctgcgcat gtgggaaaag
     9301 tgggtgttcc tggccacatt ggcggccagt acatgcttga tgcgcgggtc ggtcggcgat
     9361 attctggctg cgccggacgg caagcgcatt atcgaaaacc tgctcggcga gtgccgtggc
     9421 gtggccgagc gcaatggctt taccatgggc caggacttcg acgcccgggc cacgcagacg
     9481 ctgttcacgc cttctccgct gacggcgtcg atgctgcgcg atgtcgaaaa ccattcgcat
     9541 accgaggcgg atcacattct cggcgatctg atctcgcgcg gcggcgacgc gcaaaagggc
     9601 gaacacggct tgtcgctgct gcgtatcgcc tatagtcatc tgaagacgta cgaagcgagg
     9661 cagacccgca cgtcctgaat gaccggggcg cgggcgacgg gaacggccac agcagcttaa
     9721 cgatcggagg tgaagcatgc cgagtccaag cggctccgta cccgctctca gttccgcctc
     9781 ggcgacgatc ttctcgatcg gcatcgtctt tctcggctat tggggcttgt acgaccagac
     9841 gaaatggcga gtctccgacg tctttgtctt cgtgttcgcg atgattggct tcggctgcct
     9901 cggtctggtg ccgtggctgg cgacgagtcc cgttgaagcg gaaaccaacg acagccgcat
     9961 tcggatcgcc cggcacatgt ttcttgccgg cgtcctgtcg atctggctcg gcgtggcgat
    10021 ttcggtgatc ttctgactaa cgctcgatct gccgattcca gcgcgtatcc cactgcgcac
    10081 ggtgcgcatt gatggtgtcc gaatccacca cggtcacctt cttcaccgga tcgtcgacct
    10141 tgccaaggct ttgctgcatc ggcggcgtta tcttcgcgag acggaagaga cccgcctcac
    10201 cagcgtcgat ttcttcgcgc tggccgggct ggcggcgcag gccggcaatc cgagcggcat
    10261 gcgcacgctg tgccgtcccg cgttgttgcg ggcggaaggc gaccgtcatc gggcgatgga
    10321 ctcgaccgcc agcgaatcaa actcaatgaa caattcagcg ccaaacgttg ctgtgattgc
    10381 cgcgatgttc ttcctgggct tatttcgtcc ccaggaatac gagttgtcca gcgcgtcgag
    10441 cgcgcgcttg tactcgtccg actcaaaatc tgtcagtacg tccatcgagt aaatgacgtt
    10501 ctgcatcaga aaatcgttca cgagataccg cgacgctccc ttgaaaatca cgcgtgcatg
    10561 atctttcc
//
LOCUS       DATASH010001032         3392 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6901999,
            whole genome shotgun sequence.
ACCESSION   DATASH010001032 DATASH010000000
VERSION     DATASH010001032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6901999"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..974)
                     /locus_tag="VHV99_27455"
                     /pseudo
     CDS             complement(1..974)
                     /locus_tag="VHV99_27455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018434199.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
     gene            complement(1133..1909)
                     /locus_tag="VHV99_27460"
     CDS             complement(1133..1909)
                     /locus_tag="VHV99_27460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3639153.1"
                     /translation="MSTWILLRGLTRETRHWGRLPGLLREAIGMDRLLLLDLPGNGEF
                     ANLRAPAVVEDMVGFVRIAALQSGAAAPYRVLAMSLGGMVATDWAQRHPGEIERLVLI
                     NTSMRPFSRLHERLRPSAWPGLLGVAAHWRDAPRAESGIHRLTCNNVETLDADLKAWS
                     EIRRSAPVSRGNASRQLWAAARFCAAPTKPRSPLLILSSRADKLVNPVCSAKLAAAWG
                     APHREHQWAGHDLPHDDPAWTSEQVHAWLIEEAGEPKAAL"
     gene            complement(1906..2940)
                     /locus_tag="VHV99_27465"
     CDS             complement(1906..2940)
                     /locus_tag="VHV99_27465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEX3639154.1"
                     /translation="MQPLSFLPDSFAEYEDLKTILDQGSASFEIRTVCETTIRGRQFA
                     VHTASIGSTDPLAPAIGFFGGIHGLERIGSQLVLDYMRALLARLEWDELLVRQLQSIR
                     LIFMPIVNPGGMWAATRANPNGVDLMRNAPQNADERVPLLAGGQRVGAWLPWYRGRQG
                     EPMQPEAVALLRVVETEMSARPLSIALDCHSGYGWSDSIWFPYARTRKLMPHLAEMYV
                     LKTMFERAHPHHGYAFEPQSHQYLLHGDLWDFAYDRTPAPNVFLPMTLELGSWLWIKK
                     NPRQLFSRQGMFNPVKAHRTARVLRRHANLLDFLARAAFSSQRWLPQGNRREQLLQSA
                     IDHWTKPEAS"
     gene            3232..>3392
                     /locus_tag="VHV99_27470"
     CDS             3232..>3392
                     /locus_tag="VHV99_27470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3639155.1"
                     /translation="MEAIREAIRIERLSKTFGNGRKALDEIDLRVEPGEMVALIGASG
                     SGKSTLLRH"
BASE COUNT          663 a         1064 c         1045 g          620 t
ORIGIN      
        1 cgccggtcac cttgccgtcc gcaccggtca gcttgatgcc gacgtaatcc agatcctcct
       61 gaccgatgca ccgtcgcgcg cgagttggcc ttggtcagga agatcggtga cgagccggag
      121 atgatatcgg cggccgccgg cggcaacggc gtgacttcag cggcgccttc gagacgcagc
      181 gcgtcacgca gaatgccgag gccggagccc ggcaccgcgc gcaagccgaa gccggtgtcc
      241 tgttgcacga gttcataatc gcgctggccg agaaaggtga aatgatcggc caccatccat
      301 tcgagaaacg cgcgggcttc gacaccttcg ggccccgcct cgcccgtctt catgcctttg
      361 atggtaaggc gcgcgagttc gacgatcttc ggccagtctt ccaccgcggc gcgcacgtcg
      421 cgcagcacct tggcgatctc gtcacgcaag gcgtcgagct tcgccgcgtc gccgcaacgg
      481 tcgacttcga agtgaatgaa cgaggccagc tgcgagcgcg tgtcaccggc atcttcagcg
      541 ccctgcccca cccgcacgat gctgccatcc ggtccgcgcc agatgcggaa caccggatgc
      601 acgactgaat gcagcgcaag cccgaggcga ttgaccgcca ttgacaccga atcgaccaga
      661 aacggcatgt cgtcgttgac gatctcgatc accgtatgat cggaatgcca gccgtgctgt
      721 tcgattatcg ggttgtagac gcgcagccgt tcgctgccgg gcacaaagcg ctgcgcggtt
      781 tgccagtgcg cgagcgccgc gccgtaaagg tcggcaatcg agcggctctg cagatcgtcg
      841 gcatcgacga aatcgtagta gtgccgcaag aacggttcga cgacgttgaa ggtcggctcc
      901 ggcaaacgcc cacgtgcaaa ttcaacgaca tcgttcagca ggtgcgtgac ggcttcttca
      961 ttctttgctt gcatgatcaa ctcccccggc gggcggcaat agcgagtatc gcttgattcg
     1021 catgcatcgt ctgaacgggg attatgcgcc tgccatccgg gaaagcgatg cgcgagccga
     1081 cctgggttat acccgttggc cgccgcagcg ctgcggcagc gccgccgtct gtttaaagcg
     1141 cggccttcgg ctcaccagcc tcttctatta gccacgcgtg aacctgttcg ctggtccagg
     1201 ccgggtcgtc gtgcggcagg tcgtgaccgg cccactgatg ctcgcgatgt ggtgcgcccc
     1261 acgccgccgc gagtttggcc gaacacaccg gattgaccag cttatcggcg cgtgaagaaa
     1321 gaatcagcaa cgggctacgc ggtttcgtgg gcgctgcaca aaagcgcgcg gcggcccaaa
     1381 gctgccgcga cgcattgccg cggctcactg gcgcgctccg gcgaatctcg ctccatgcct
     1441 tgagatccgc gtccagcgtt tcgacgttat tgcacgtcag ccgatgaatg ccgctttccg
     1501 cgcgcggcgc gtcgcgccaa tgggctgcga cgcccagcag gcccggccag gcggatggac
     1561 gcaagcgctc gtgcaggcgg ctgaacgggc gcatgctggt attgatcagt acgagccgct
     1621 cgatctcgcc gggatgccgt tgtgcccaat cggtcgcgac catgccgcca agcgacatcg
     1681 ccagcacgcg gtagggcgct gccgctccgc tttgcagcgc agcaatccgc acgaagccca
     1741 ccatatcctc gaccaccgcc ggtgcgcgca agttcgcgaa ctcgccgttg ccgggcagat
     1801 cgagcagcaa cagccgatcc atgccgatcg cctcgcgcag caagcccggc aaacgccccc
     1861 aatggcgtgt ttcgcgcgtc agtccacgca acagaatcca cgtactcatg acgcttccgg
     1921 cttggtccaa tggtcgatcg cgctttgcag caattgctcg cgacgattgc cttgcggcag
     1981 ccagcgttgc gatgagaacg ccgcgcgcgc gaggaaatcg agcagattcg catgacgccg
     2041 cagcacccgt gcggtgcgat gcgctttcac tggattgaac ataccctgac gtgaaaacag
     2101 ttggcgtgga tttttcttga tccatagcca tgaaccgagt tcgagcgtca tcggcaggaa
     2161 gacgttgggc gccggcgtac ggtcgtacgc gaagtcccac agatcgccat gcaacaaata
     2221 ctgatgactc tgcggctcga acgcatagcc gtgatgcgga tgagcgcgct cgaacatggt
     2281 tttcagcacg tacatttccg ccagatgggg catcagtttg cgggtacgcg cgtagggaaa
     2341 ccagatgctg tcgctccagc cgtaacccga gtggcagtcg agcgcgatgc tcaggggccg
     2401 cgcgctcatt tcggtttcaa ccacccgtag gagtgccacg gcctccggct gcatcggctc
     2461 gccttgccgc ccccgatacc agggcagcca tgcgccgacc cgctgaccgc cggcaagcag
     2521 cggcacgcgt tcgtcggcat tttgcggcgc gttgcgcatc aggtcgacac cattgggatt
     2581 ggcgcgcgtg gcagcccaca tgccgcccgg attgacgatc ggcataaaga tcagacgaat
     2641 cgattgcaac tgccgcacca gcagttcatc ccattccagc cgggcgagca gcgcgcgcat
     2701 gtagtcgagc acgagttgcg agccgatccg ttcgagcccg tgaatgccgc caaaaaagcc
     2761 aatagccggc gcgagcgggt ctgtcgaacc gatactcgcc gtgtgcacgg cgaattgccg
     2821 gccacgtatc gtcgtttcgc agacggtacg gatctcgaag ctggcgctgc cctggtcgag
     2881 gatcgtcttg agatcttcgt actccgcaaa actgtcggga agaaagctca aaggctgcat
     2941 gacgacgtcg caaggaactg caaggttatt ggggacggca cgccggatga cgcaatatag
     3001 cctggctgca ggcgatcatg cgttgccggc accgcgcgag gcaaatcgtg tcatacgggt
     3061 gtcatttttt attcgaatga atgacttctg tttgccttcg tggcgacgca aaaatgacac
     3121 atggagatat agtcgtctag acgttgcgac accttcactg aaccgtcaca gatgcttcct
     3181 agaatgagcc ccacacatgc cggcaatggc cggatgcatc aagggatatt tatggaagca
     3241 attcgggaag caattcgtat cgaacggttg agcaagacgt tcggcaacgg ccgcaaggcg
     3301 ctggacgaaa tcgatctgcg tgtcgagcca ggcgaaatgg ttgcgttgat cggcgcctcg
     3361 gggtcgggca agtcgacgct gctgcgccat at
//
LOCUS       DATASH010001033         4050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2945825,
            whole genome shotgun sequence.
ACCESSION   DATASH010001033 DATASH010000000
VERSION     DATASH010001033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2945825"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(385..954)
                     /locus_tag="VHV99_27475"
     CDS             complement(385..954)
                     /locus_tag="VHV99_27475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX3639156.1"
                     /translation="MLIAVGALASSLSLSAFAAADTYQLDPTHTYPSFEADHFGGLSV
                     WRGKFTKSSGTVTLDRAAKTGTVDVTVDPASIDTGNSKLDEHLKTDAFFDVAKYPSVT
                     YKGTDIKFDGDKPVEVIGNLTIRGITKPLNLKIESFKCMQHPVLKREVCGVEASAHFS
                     RADYGMDFGAKYGFSMDTKLHIQSEGIKQ"
     gene            complement(1033..1596)
                     /locus_tag="VHV99_27480"
     CDS             complement(1033..1596)
                     /locus_tag="VHV99_27480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015001637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX3639157.1"
                     /translation="MKVSFYRYMLAAFAAASLSAAGSALAQVDVAKSSVTATSKQMNV
                     PVEGKFGKFTAQVKFDPAKPAEGTAQFSIDVGSYDLGDESYNDQVRGKDWFDAKTFPT
                     ATFVSSAIAPAGGNQFKVTGKLTIKGKSQTVVVPVTVTQLGATQTFDGSLPIKRSQFD
                     IGTGEWKDTSVVADDVVIKFHIVAARK"
     gene            complement(1605..2165)
                     /locus_tag="VHV99_27485"
     CDS             complement(1605..2165)
                     /locus_tag="VHV99_27485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEX3639158.1"
                     /translation="MALNPSFGGRESYTRTAIAFHWLIALLIVCGFSLGWVMTDIPGF
                     TPTKLRYFSWHKWIGVTVFALAMLRILWRATHAAPPLPRRMPAWQRGAAHLTHLLLYV
                     LMIVIPASGYLYSSAANVPVVYLGLIPLPRLIAPDPHLKELLKNVHIALNYTLLVLVA
                     LHVGAALKHQWLDRDGLLSRMLPFIK"
     gene            2578..3321
                     /locus_tag="VHV99_27490"
     CDS             2578..3321
                     /locus_tag="VHV99_27490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEX3639159.1"
                     /translation="MEQDNLIACHECDTLFRKPRLVGRASARCSRCGATLYRGISRKL
                     DSICAMTLAALITFIIAQAFPIVELETNGITSQTTLFGALVALWGEDMQIVAVMVFCS
                     TILFPLTELVALLYVLIPLRAGYVPPAFNRVLRAIQFVRPWGMIEVFMLGVLITIVKM
                     VSLARVIPEAALFAFGVLTLMIAVVLTFDPRVLWDLADEMGERGRRPLPRTSADSAAA
                     AVGSLAGPPAPPDVQGAAQSTLPPAPRQG"
     gene            3329..3991
                     /locus_tag="VHV99_27495"
     CDS             3329..3991
                     /locus_tag="VHV99_27495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEX3639160.1"
                     /translation="MKYTTAKRAGLVACHSCGRVEPRIRSVTPQHCGRCGAHLHSRNP
                     DSLMRTWALLIAAALLYIPANLLPVMHTTSLLGSEDDTIMSGVVYFWTSGDWPLAVIV
                     FIASIMVPMLKLSVLVLLTATCQRRSRWRPEQRTTLYRMVERIGRWSMLDVFVVTLTV
                     ALVRFKSLAVITAGPGAIAFGSVVILTMMASMQFDPRLIWDDVEGGVNGSTQKPQDLD
                     HD"
BASE COUNT          762 a         1255 c         1235 g          798 t
ORIGIN      
        1 ccattccagc gcgttgccga tcgaggcggc gaccaccgcg cgccacgaat tctgctggct
       61 ccccgccagg cttgccgctg tcgttgtatt catgcagatc tccaatgcgc gaaatattat
      121 ttacgtaggt tctaccgtga ctattgcccg aactggatgc gcgcacaacc aatacgatgc
      181 cagcgctcta tgtacctgaa aaatcaaaac gacgcgagcg gcaaataggc ggtggccact
      241 gcacaaggct tcgcgcgctt gaagtgggca ataaaaaaac cgcttcatcg caaaagcgaa
      301 cgaaacggtt tttttgttgc ggcgcaatgc gccgcactca caagcggccc ttgaacgcga
      361 atgcgcaatg gctccctaag gcccttattg cttgatgcct tcggactgaa tgtgcagctt
      421 ggtatccatg ctgaagccgt atttggcgcc gaaatccatg ccgtaatccg cgcggctgaa
      481 atgagcgctc gcttccacgc cgcacacttc gcgtttgagc accgggtgct gcatgcactt
      541 gaacgattcg atcttcagat tcagcggttt ggttatgcca cggatcgtca ggttgccgat
      601 cacttcaacc ggcttgtcgc cgtcgaactt gatatcggtg cctttgtagg tcaccgacgg
      661 atacttggcg acgtcgaaga atgcgtccgt cttcagatgc tcgtcgagct tgctgttgcc
      721 ggtatcgatc gaagccgggt cgaccgtgac gtccaccgtg ccggttttcg cggcgcggtc
      781 gagcgtgacc gtgcccgagc ttttggtgaa cttgccacgc cacaccgaga ggccgccgaa
      841 gtgatccgcc tcgaagctcg gatacgtgtg agtcgggtcg agctgatagg tgtcggcagc
      901 agcaaacgcg ctgagcgaca aagaagaagc cagtgcgccg acggcgatca acagggatgt
      961 tttcaattta tgctccttgt gtttccggaa tgcgtgaagg atagcggttg cgcctgaagc
     1021 gcgccgcgaa cgtcacttgc gtgctgcgac gatatgaaat ttgatcacca cgtcatcggc
     1081 gaccaccgag gtgtctttcc attcgcccgt gccgatgtcg aactgcgaac gcttgatcgg
     1141 caacgagccg tcgaacgttt gcgtcgcgcc cagctgggtg accgtgaccg gcaccaccac
     1201 cgtctgcgat ttacccttga tggtcagctt gccggtgacc ttgaactgat tgccgccggc
     1261 cggcgcaatc gcgctcgata cgaaggttgc agtcggaaag gtcttcgcgt cgaaccagtc
     1321 cttgccgcgc acctggtcgt tgtagctctc gtcgcccaga tcgtagctgc cgacgtctat
     1381 gctgaactgc gcggtaccct cggccggttt tgccggatcg aacttcactt gcgcggtgaa
     1441 tttaccgaat ttgccctcca ccggcacgtt catctgcttc gaggttgccg tcaccgagct
     1501 tttggcgacg tcaacctgcg cgagcgcact gcccgccgcg ctcagcgagg ccgccgcgaa
     1561 cgcggcgagc atgtagcgat aaaacgaaac tttcatggtt gtccttattt gatgaaaggg
     1621 agcatgcgcg acagcaggcc gtcgcggtcg agccattgat gtttgagcgc cgccccgaca
     1681 tgcagcgcga ccagcacgag caatgtgtaa ttcaacgcga tgtgcacatt cttcagcagt
     1741 tccttgaggt gcgggtccgg tgcgatcagg cgcggcagcg gaatcaaacc gaggtacacc
     1801 accggcacat tggccgccga gctatacaga tagccggagg cgggaatcac gatcatcagc
     1861 acgtagagca gcagatgcgt cagatgcgcg gcgccgcgct gccaggccgg catgcggcgc
     1921 ggcagcggcg gggccgcgtg ggtggcacgc cacagaatgc gcagcatcgc gagcgcgaac
     1981 accgtcacgc cgatccattt atgccatgag aagtagcgca gcttggtggg cgtgaagccg
     2041 gggatgtcgg tcatcaccca accgagcgag aagccgcata cgatcaacag cgcgatcagc
     2101 cagtgaaagg cgatggcagt tcgcgtgtac gactcccggc cgccgaaaga aggattgagt
     2161 gccatggcag gtgaacgcaa gctaagagag gttaacggcg caaccggtcc tgctattcca
     2221 cgctgcccca tgcatgctgc atggtgcgaa tcgaaagaaa gcacagcggc tgcggcacac
     2281 gtcaggccgc catgctaccg caagcgggag actctggctg cggcggtcga catgtggtca
     2341 gacggaaagt tttcccgtac gcaaacggta ttgaacaatt ggggacggag actgtcagaa
     2401 ccggagcaat ttatgtaaga ctagtctgaa tgccggcaac acccgagcct caacggcaac
     2461 gaggggaatg gccggtccgg tgtcaaagcg cgtttggtac tattgctcca cgttttgtcc
     2521 agcgttgccc tgaaaccggc attgcacgcc tctcttacgt cgttagcccg cagccgtatg
     2581 gaacaagaca acctgatcgc gtgccatgaa tgcgatacgc tgttccgcaa gccccggctt
     2641 gtcgggcggg cgtccgcgcg ctgttcgcgc tgcggcgcga cgctctatcg cggcatctcg
     2701 cgcaagctcg acagtatctg tgcgatgacg ctggccgcgc tgatcacctt catcatcgcg
     2761 caggccttcc cgatcgtcga actcgagacc aacggcatta cctcgcagac cacgctgttc
     2821 ggcgcgctcg tcgcgctgtg gggcgaggac atgcagatcg tcgcggtcat ggtgttctgt
     2881 tcgaccatcc tgtttccgct gaccgagctg gtcgcgctgc tctacgtgct gatcccgctg
     2941 cgcgccggct acgtgccgcc cgccttcaac cgcgtgttgc gcgcgattca attcgtgcgg
     3001 ccgtggggca tgatcgaagt gttcatgctc ggcgtgctga tcaccatcgt gaagatggtg
     3061 agtctcgcgc gcgtgattcc cgaagccgcg ctattcgcct tcggcgtgct gacgctgatg
     3121 attgcggtgg tcctcacatt cgatccgcgc gtgctgtggg atctcgccga cgaaatgggc
     3181 gaacgcggcc ggcggccgct gccgcgcacc tccgcggaca gtgcggccgc ggctgtcggt
     3241 tcgctggccg ggccgcccgc gccgcctgac gtgcaaggcg cggcgcaatc cacattgccg
     3301 ccggcgccgc gtcagggatg agcacacgat gaaatacacg accgcgaaac gcgcgggtct
     3361 ggttgcttgc cactcgtgcg gacgcgtcga gccacgtatc cgctccgtca caccgcagca
     3421 ctgcggacgc tgcggcgcgc acctccactc gcgcaacccc gatagcctga tgcgcacctg
     3481 ggcgctgctg atcgccgcgg ccttgctgta tattcccgcg aacctgctgc cggtgatgca
     3541 tacgacgtcg ctgctcggtt cggaagacga caccatcatg agcggcgtcg tgtacttctg
     3601 gacctcgggc gactggccgt tagccgtgat cgtgttcatc gccagcatca tggtgccgat
     3661 gctcaagctc agcgtgctgg tactgctcac cgccacttgc cagcggcgct cgcgctggcg
     3721 gccggaacag cgcaccacgc tctaccgcat ggtcgaacgg atcgggcgct ggtcgatgct
     3781 cgacgtgttc gtcgtcacgc tgaccgtcgc gctggtccgt ttcaagtcgc ttgctgtgat
     3841 cacggccggc ccgggcgcga tcgccttcgg ctcggtggtg attctgacca tgatggcctc
     3901 catgcaattc gatccccggc tcatctggga tgacgtggaa ggcggcgtga acggcagtac
     3961 ccaaaaacct caggacctcg accatgacta gcccgccagg accgacgccc gcctccgacg
     4021 cgccgcgcaa cgattcgaac ggaccgaagc
//
LOCUS       DATASH010001034         3665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6902048,
            whole genome shotgun sequence.
ACCESSION   DATASH010001034 DATASH010000000
VERSION     DATASH010001034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6902048"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..1601)
                     /locus_tag="VHV99_27500"
     CDS             complement(186..1601)
                     /locus_tag="VHV99_27500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEX3639161.1"
                     /translation="MSSGSPLLSSSIVRRARRLWRQYGVFWLGAIAVGLVAVLYARLI
                     DWGYGEFRTMQQQHVWAPLIVTPAVAALAVWLTRTFFRGAEGSGIPQVIATLHARPGE
                     YGARLLSFRILFGKIAVSFLAILGGFTIGREGPTVQVGAALMFNLRRLYPRSNAQIER
                     QLVLAGAAAGLSAAFNTPLAGIVFAIEELTRSFEARASGVLITAIIIAGVIALGLNGN
                     YTYFGTIQIGAHFPDLLAVAVVLTAIVTGIAGGVFGWLLLNTARWIPAPLRQLHGARP
                     VAFAALCGFVIAVVGLISGGTTFGSGYAEARGLLDGNEHLSVFYPFLKMISMVGSYLP
                     GIPGGIFAPSLSIGAGFGNLLHMVFSSMQLPMLIALAMVGYLAAVTQSPITSFVIVME
                     MIDGHALVISLMATALIASRVSRFFAPPLYESLAQRYMAPLPQPAPAPVPEVPEVEAA
                     EPAVVDETNGDASTEEGAPRQ"
     gene            1811..2815
                     /gene="waaC"
                     /locus_tag="VHV99_27505"
     CDS             1811..2815
                     /gene="waaC"
                     /locus_tag="VHV99_27505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HEX3639162.1"
                     /translation="MKRVLIVKVTSLGDIVQALPVVADIKRAFPGVEVDWAADEAFAE
                     VVHWSESVDRVLSAPLRRFKKARHWGDFKAIAASIAELRAYRYDFIIDIHGVYKSAII
                     AFLARSSKRIGYQSQDLGERGAAFAYTGRFGPRPQCNAWHGMRISAGEALGYEVEGPA
                     VYNLRLPDPATAPFAADSAPVAALFHATSKDDKKWPVPHWVTVGRELVQRGFRVVLPW
                     GSEGERAEAEQIASEVPGATVLPRLSVTEIAQMIDACALVVGTDTGFVHLAHALQKRT
                     VMIFVATSPSHCGIEAPFRSISIGDGHSVPSIAEALEAIAYVNTEPRIDATQHGSAAA
                     "
BASE COUNT          704 a         1142 c         1135 g          684 t
ORIGIN      
        1 tgacgtgctg tgagttctgc ttcgttgcgt cgatgtgatg tgacgaggta gagcgcgtcg
       61 gcgccgttct ccaccggttc tttctgctgt tcaatttgac gcgcggatgc gaattctacg
      121 cgctgtctgt cttgttgtgc ctttctctct gcagcgcgcc ggcgcctacc gtctactaac
      181 gtcgtctact ggcgcggggc gccttcctca gtggaagcat caccgttggt ctcatcgacc
      241 accgctggtt cggccgcttc gacttcgggg acttccggca ccggcgcggg cgccggttgc
      301 ggcagcggcg ccatatagcg ctgcgccagc gactcataca acggcggcgc gaagaaccgc
      361 gacacccggc tcgcaatcaa cgcggtggcc atcagcgaaa tcaccagcgc gtggccgtcg
      421 atcatctcca tcacgatcac gaacgacgta atcggcgact gcgtgacggc ggccagatag
      481 ccgaccatcg cgagggcgat cagcatcggc aactgcatcg aactgaacac catgtgcagc
      541 agattgccga agcccgcgcc gatcgacagc gacggcgcga agatgccgcc cggaatgccc
      601 ggcagatacg agccgaccat tgagatcatc ttcagaaacg gatagaacac ggagaggtgc
      661 tcgttgccgt cgagcagtcc gcgcgcttcc gcatagccgc tgccgaaagt cgtgccgccg
      721 gaaatgaggc cgaccaccgc gatcacgaaa ccgcacagcg cggcgaacgc gaccggccgc
      781 gcaccgtgca actgacgcag cggcgccgga atccagcgcg cggtgttcag cagcagccag
      841 ccgaacacgc cgcccgcaat accggtgacg atggccgtga gcacgacggc caccgccagc
      901 agatccggga aatgcgcgcc gatctgaatg gtgccgaaat aggtgtagtt gccgttcaac
      961 ccgagcgcga tcacaccggc gatgatgatc gccgtaatca gcacaccgct tgcacgagcc
     1021 tcgaagctgc gcgtgagttc ctcgatcgcg aacacgattc ccgccaacgg cgtattgaac
     1081 gccgcggaca aacccgctgc cgcgcccgcg agcaccagct gccgttcgat ctgcgcgttc
     1141 gaacgcggat acagccgccg cagattgaac atcagcgccg cgccgacctg cacggtcggt
     1201 ccttcgcggc cgatcgtgaa gccgccgaga atcgccagaa acgacactgc gatcttgccg
     1261 aacagaatcc ggaacgacag cagccgcgcg ccgtattcgc cgggcctggc gtgcagggtg
     1321 gcaatgactt gcgggatgcc gctgccttcg gcgccgcgga agaatgtgcg tgtgagccag
     1381 acggcaagcg ccgcgaccgc gggcgtgacg atcaacggtg cccacacgtg ctgttgctgc
     1441 atcgtgcgga actcgccgta accccagtcg atcagccttg catacaggac cgccaccagg
     1501 ccgactgcga tcgcgccgag ccagaagacg ccgtattgcc gccacaggcg tctcgcgcga
     1561 cgcacgatgc tggaagagag aaggggagat ccggaggaca tgagaatacc ggcgcaaagg
     1621 gtgaagtata aggcgcgccg gaccggttca acggtgtgaa agtattctga aatgatgtaa
     1681 aagggttctc gtaagtgtgc aatgtaacgc tctgtattga tgaccgcatt cgcgccttgc
     1741 tgccttcagc aaagattctt tcaatcgtcg gctaaaatgg tttgacgttc gaacaactaa
     1801 taggtcctca atgaagcgtg tccttatcgt caaggtcact tcactcggcg atatcgtgca
     1861 ggcgctgccg gtcgtcgccg acatcaaacg cgcgtttccc ggcgtggaag tggactgggc
     1921 agccgacgaa gcattcgccg aagtggtgca ttggagcgag agcgtggacc gcgtgctgtc
     1981 cgcgccgctg cgccgcttca agaaagcgcg gcattggggc gatttcaagg cgattgcggc
     2041 gtcgatcgcc gaattgcgcg cctatcgcta cgacttcatc atcgacattc acggcgtcta
     2101 caagagcgcg atcattgcgt ttctggcccg ttcgtcgaag cgtattggct atcagtccca
     2161 ggacctggga gagcggggcg ccgcatttgc ctacaccggg cgtttcgggc cgcgtccgca
     2221 gtgcaatgca tggcacggca tgcgcatcag tgccggcgag gcgctgggtt atgaggtcga
     2281 aggcccggct gtctacaatc tgcgcctgcc cgaccctgct actgctccgt tcgctgcaga
     2341 cagcgcgccg gtcgcggcgc tgtttcacgc cacctccaaa gacgacaaga aatggccggt
     2401 gccccattgg gtcacggtcg gtcgcgagtt ggtacagcgc ggttttcgcg tggtgctgcc
     2461 gtggggctcg gaaggcgagc gcgccgaggc ggagcagatt gcgtctgagg tgccgggcgc
     2521 gaccgtgctg cccaggctga gcgtgaccga aatcgcgcag atgatcgacg cgtgcgcgct
     2581 agtagtcggc accgataccg gcttcgttca tctggcgcat gcattgcaga agcgcacggt
     2641 catgattttt gtggccacct cgccgtcgca ttgcggcatc gaggcgccgt tccgctccat
     2701 ttcgatcggc gacggtcatt ccgtaccgtc gatcgccgag gcactcgagg caatcgccta
     2761 cgtgaatacg gagccacgca tcgacgccac gcaacacgga tccgccgccg cctgagtgca
     2821 gcttcgttgc gttttcgcgc aatcgtttgg cgaaaagaaa agaggcgtga cgcgcagtcg
     2881 cccaatcaaa caagcgctgc cacggaacgc ctcgaaaaac gcccgtgtcg gggaactacg
     2941 ggcggagagg aatcgctgca atgaacaaac tgactcaagc gccgggcggc cacatcgcgt
     3001 gacgcgcctg gctctaaaga gaccgccggc cggcggtcga gttcaatcgt ctcttcatca
     3061 aggcatgccg cgcgcgtgcc ggcggattcg tgtaccgctc atcggcgtcc cgcagccggt
     3121 cgcgcatcgc attcgcgcgc gttccctgcg ttcggttacg catcgcgcat tacacgcctg
     3181 ttaaatctgt cgcttttcgt tgagtgccta attggctaca cgtactctct cgtgcgcggc
     3241 tgccggctca tttatcgcgc atgcaacgcg aacgctatcc tcacaatcgt cgcgtcggcc
     3301 cgcgcatccg ccaccggcag ggcttgtgcg ccgcgcacat gttcaaggca gtataggcgg
     3361 tgttttcgtc tgaagtgaat ctcttgtcac cctcgtcgcg cggggccggt ttcggactgc
     3421 gcgcgtgtaa cacatcggtg atcttcactt acaaatagca acgttcgtgc gaggccgggt
     3481 cgtgtccaat cgagtcgttc ggggtttcgg ctcgaatgaa tctgactcga aggtgaacaa
     3541 tatgaaacgc ttaatcctga ctctcgtcac aggcacgctg ctcgcaacgg cgcttggagg
     3601 ttgcatcgtt gcaccggcgc ctggctatta ctacggcggc ggtggttatt accatcacgg
     3661 tggct
//
LOCUS       DATASH010001035         5570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3344739,
            whole genome shotgun sequence.
ACCESSION   DATASH010001035 DATASH010000000
VERSION     DATASH010001035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5570
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3344739"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..546
                     /locus_tag="VHV99_27510"
     CDS             73..546
                     /locus_tag="VHV99_27510"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEX3639163.1"
                     /translation="MRYHDDVPEHVVSANNAGTTQRSTRRTVLTRALALVASISLLSS
                     LIPMTAEAAAEKGSGPAIVNVVTFKVPSAGMADFLRISKENSQASRKEEPGCIGFDVL
                     LPNDEPNTVLLIETYRDEAAYRAHRVTPHFLAFVKRAQEIGAKRSAHVGRRYYPA"
     gene            complement(1214..2371)
                     /locus_tag="VHV99_27515"
     CDS             complement(1214..2371)
                     /locus_tag="VHV99_27515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017332716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX3639164.1"
                     /translation="MKSKLLSLAALAAVSSVAHAQSSVTLYGTLDTAVAYFSNQKGAH
                     TSGGTFQMVSGTLTGDNWGVKGTEDLGGGLSAVFNLQSGYNLNTGAAGQGDRMFGRTA
                     MVGLADKSYGTVTAGRQYDPLVDLIQPLTEDDTYGSAFATPGDIDNYDNSSRVNNSIK
                     YTSPTYAGLQVSAMYAFGGEAGETSGGQTYAIAALYSHGPLNLAAGYLRANNNGTSST
                     YDGFTSTGLDSTAINGAFITATTMQIIRAAATYAIGPVTLGAAYSNVDYGNYAAAPGP
                     NNGTRFSTGQAFVNYQLNAAVELGVGYNYTKGSGNNVDASYSQVSLGADYALSKRTEL
                     YALAAYQKASGNTIDASTGQVVAATASVGDFGNDASTNNQTMVMLGIKHHF"
     gene            complement(2603..2839)
                     /locus_tag="VHV99_27520"
     CDS             complement(2603..2839)
                     /locus_tag="VHV99_27520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639165.1"
                     /translation="MLEDPDLERAATLTVQGPFKESEQRCAAVKQMLALVDKVRAWTY
                     GGSRKWDDAALDANGHTPEVEHFIGVAERLMAQR"
     gene            3040..3156
                     /locus_tag="VHV99_27525"
                     /pseudo
     CDS             3040..3156
                     /locus_tag="VHV99_27525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011546896.1"
                     /note="catalyzes the formation of phosphonoacetaldehyde
                     from 2-aminoethylphosphonate and pyruvate; incomplete;
                     partial in the middle of a contig; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
     gene            3906..4787
                     /locus_tag="VHV99_27530"
     CDS             3906..4787
                     /locus_tag="VHV99_27530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HEX3639166.1"
                     /translation="MDDDAHAFNGIRPRLQKIAYRMLGSVAEAEDIVQDVWRRWHAAA
                     SGGLDNAEAWLVAVTTRMSIDRLRAAKIQRAHYAGIWIPEPQMTDFPTTPEEATERAD
                     DVSVAYLMLLERLTPEARAAFLLREVFDADYGEVAQAIGKTEAACRQLVSRAKVQLRD
                     ERPRYTVSRETHLRLLRTFAQALERGDFHSINGLLAEDATLIGDGGGKVPSFAKPMVG
                     GRRIAQLFYASSLRHGSELGVKLVLLNGQWALLRFIEGKLESAQSFETDGERIIRIHV
                     QRNPDKLARISAAHSSG"
     gene            4971..5450
                     /locus_tag="VHV99_27535"
     CDS             4971..5450
                     /locus_tag="VHV99_27535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX3639167.1"
                     /translation="MTQRLNYFQQSPALVKKFIELNNLLKDGTIEESIRDLVSIRASQ
                     LNGCGFCLDMHVKEAHIHGERELRIHHLAAWRESTLFAPRERAALAWTEVLTHLPEHG
                     VPDDIYERVRTQFSEKELSDLTFDVMAINGWNRANVAFKMVPGSFDKAFGLDKANLA"
BASE COUNT         1120 a         1673 c         1641 g         1136 t
ORIGIN      
        1 tctccacgat ggctcatggg acggtcttcg taacaatcaa cgctactcgc actgagaaag
       61 gatggctgga tcatgaggta tcacgacgat gtgcccgagc atgtggtctc tgccaataac
      121 gcaggcacaa cgcagcggtc aacacggcgc acagtcctga cgcgcgcgct ggcgttggtg
      181 gcgagcatat ctctactgtc cagtttgatc cccatgacgg cagaggctgc cgccgagaaa
      241 ggatcggggc ctgccattgt caatgtcgtt acgttcaaag tgccatccgc aggaatggca
      301 gacttcctca gaatcagcaa ggaaaacagc caggcatcgc ggaaggaaga gccgggctgc
      361 ataggcttcg atgtgctgct tcccaacgac gagcccaata cagtcttgct gatcgagact
      421 tacagagatg aggccgccta tagggcccat cgggtgacac cgcatttcct ggcattcgtg
      481 aagcgcgctc aggagatcgg agcaaagcgg tccgctcatg ttggccgccg ctactatccg
      541 gcctgacggt cttctcgaac gagatcggtt ctcgaacatc acgggcgcga aagaggcggc
      601 cggcggcaca aaatgaaggc ggttctgtcg cgataagggc gagaaggcga caaaaatgtc
      661 acgctccttc ggttgatctg ccttgcgaca cgaaagcatt gttcccgccg cgcgagcggt
      721 ttcgttcagg cgaccctatc cggcctttcg agccagccga accgtgacgg cagctttcaa
      781 ggggcggagc ggacgttcga agttccgccg ggcggcacga tagccgcaac tggccagtcg
      841 cggctatcgt gctgaacctg ccgcctatgg cagtgacgag cgctgcgtgc aacctcgcaa
      901 tgtgcatgtg gttgagcgca cgcacgccgg gttggcccgc gccaaggcac tacaggattt
      961 ctgttcgtcc gtcgtcgctg acacctgggc aacgcgtcga aatcaaggtg aagccagcgg
     1021 cggacgtgcc gatttgctca gtggctccgt ccgcgatctc cagcggtatc tgcgacgcgc
     1081 tcggcatccc cctacggcag tgccatttat gccaatggaa gcacaggcga cgctgaaagc
     1141 gtggcgagat gcgaaaccgg tcaatagtcg caactccagc gtcgccctcg ttgacggtgc
     1201 tcagttagac gaatcagaaa tggtgtttga tgccaagcat caccattgtc tggttgttcg
     1261 tgcttgcgtc attgccaaag tcgccaacag acgccgttgc agccaccacc tgaccggtcg
     1321 acgcgtcaat tgtgttgccg gaggcctttt gataggccgc cagagcatac aattcggtgc
     1381 gcttggacaa cgcgtagtcc gcgccaagac tgacctggct gtagctggcg tcgacgttgt
     1441 tgccgctgcc cttcgtgtag ttgtagccga ccccgagttc gacggcagcg ttcaactggt
     1501 agttaacgaa cgcctgcccc gtgctgaacc gggtgccgtt gttcgggcct ggggcagccg
     1561 cgtagttgcc gtagtcaacg ttgctatagg cagcgcccag cgtaaccgga ccgattgcat
     1621 aggtcgcggc ggcgcggatg atttgcatcg tggtcgcagt aatgaacgct ccgttgatgg
     1681 ccgtcgagtc gaggccggtc gaagtaaagc cgtcgtacgt cgaggacgta ccattgttgt
     1741 tcgcacgaag ataaccggca gccagattga gggggccatg gctatagagc gccgcaattg
     1801 cgtaggtctg tccgccgctc gtttcgccgg cttccccgcc gaaggcgtac atggctgaca
     1861 cctgaaggcc agcatacgtc ggactggtgt atttgattga gttgttgaca cgggaactgt
     1921 tgtcgtagtt gtcgatgtcc ccgggggttg caaaggccga cccataggta tcgtcctctg
     1981 tcagcggttg gatcaagtcg accagcgggt catattggcg ccccgcagtg acggtgccgt
     2041 agctcttgtc agcgaggcca accattgcag ttcgaccgaa catgcggtcg ccttgtccgg
     2101 cagcgccggt gttcaggtta tatccggatt gcaggttgaa cacggccgac aggccgccac
     2161 caaggtcttc agtacctttc acaccccagt tgtcgccggt aagcgtgccc gataccatct
     2221 gaaacgttcc acctgacgtg tgcgctccct tctggttact aaaataggcg accgccgtgt
     2281 cgagcgtgcc gtacaaggta acactgcttt gagcgtgcgc taccgaggag accgcagcga
     2341 gcgcggcgag cgagagaagc tttgatttca tgatgtgttc cgatgtgtat gtgtcactgt
     2401 ctggtaccgt cgtcgcctcg catgccactt tacggacgca tcaaaatagc gtgagcttca
     2461 ttccaaacgt cgaaaacatc ggattttctt tcacgtagta aggcatccta cattttgaat
     2521 ttgatcgatc aagaaaattg accgttgaaa cacattctgt tcggcagcac gccgcagtat
     2581 tacgacgccc gacgcgcgct ccctaccgct gggccatcag acgctccgcc acgccaataa
     2641 agtgctcgac ctcgggcgta tgcccgtttg catccaatgc ggcatcgtcc cacttgcgcg
     2701 aaccgccata cgtccatgcg cgcactttgt cgaccaaagc aagcatttgc ttgaccgccg
     2761 cgcagcgctg ctccgactcc ttgaacggac cctgcacggt gagcgtcgcc gctcgttcga
     2821 gatcgggatc ttcgagcatg acgagcggat agttgtcgcc gaactcaagc ccgtagcggc
     2881 tcgtctcgcc gtcgcccgtt cagccccggc aagaattaca aggtttggtg tcagccagat
     2941 gacgaaaggg gatgtttcca tagttcgaca caatttgcca tttattgaca cattgacaat
     3001 ttgttcgact ggaggttttg cgatgcgtga acacacccca tcctgccaac gccgcgatcg
     3061 ttcacgacat cgcccgacac gaggcacgcg atgccgcgcg agcggggctc atgggatacg
     3121 gcattcaatt gtctgacact gagtgtctgc ggcgatcaac agcgcatacc gcgtacatcg
     3181 tgcccgtctt gcagcgcttg ggaatcgcgt atgtagagtt gccgcttccc gagccggccg
     3241 ctgcgtgcgc aaggccacgg tcgcttcgac gcaacgagcg gcgtcctcgg tcgcgaccac
     3301 gtcggggacg atatagccga ttttcggatc atcggaaagc cagtgtctgc aacggcgctt
     3361 cacgccgtcc cgagacgaca accgtggcgc tgcgccccgc gaacatcagt gccgcctaac
     3421 gtccgatgcc tgcgccgccg ccggtgatta gcacgacctt gtcgtcgaag tcattcatgt
     3481 tgtgttcgct cttcacatcg cctccgctga ccggccaacg gcaagccgat acgcgatctg
     3541 tatgacgcgg cgctccaaat gcccagacgt gcgcaaaccc gcgagacgcg ccggttcctc
     3601 cactcccaag ccggcatccg actgggccgc cgcgcgtgaa atgaagccat gacggttcaa
     3661 caattcgtct cttgtcgtgg ggggcctatg tccaatccga tcagggattt tgaccgcgaa
     3721 gctcactcat cgggcacgaa tgcccactct cgcctcaacc aatcgaaccg caacggctcc
     3781 ggtttctgta gatcaaaaac gcaatgccgg gcgtctcaag cgttgtctca cccgtggcaa
     3841 tatgccactg tcagcaatct gttcaggcag cggtttgtgc cttttagcgc aaggggatcc
     3901 ggttcatgga tgatgacgca catgccttta acgggattcg cccccgactg cagaagattg
     3961 cgtatcgaat gttagggtcg gttgccgagg ccgaagatat cgtgcaggac gtctggcgtc
     4021 gttggcatgc ggcggccagt ggaggtctcg ataatgcgga agcgtggctg gtcgcggtca
     4081 cgacacggat gtccatcgac cgtctgcgcg cggcaaagat tcaacgcgca cactacgcgg
     4141 gcatatggat acccgaaccc cagatgacgg acttcccgac tacgccagag gaggccacgg
     4201 agcgcgccga cgacgtctcc gtggcctatc tgatgctgct cgagcggctg acgccggaag
     4261 cccgggcagc attcctgttg cgcgaggtct tcgacgccga ctatggcgaa gtcgcacagg
     4321 ctatcggcaa gacagaagcg gcgtgccgtc agctggtaag ccgtgcaaag gtgcaactac
     4381 gcgacgagcg cccgcgttat accgtttcgc gcgagacgca tcttcgcttg ctgcggacgt
     4441 tcgcgcaggc gttggagcgc ggggacttcc actcgatcaa cggattgctg gccgaagacg
     4501 ctacgctgat tggcgacgga ggcggcaagg tcccgagctt cgctaagccg atggtaggcg
     4561 gccggcgcat cgcacagctt ttctacgcgt cgtcgctgcg tcacggcagc gaactcggcg
     4621 tcaagctcgt gctgctgaac ggccaatggg cattgctgcg ctttatcgaa ggcaaactcg
     4681 aatcagccca gtcgttcgaa acggacggcg agcgcattat tcgtatccat gtgcagcgca
     4741 acccggacaa gctcgcccgc atctcggcag ctcactccag cggctgaatg ccgacctcgg
     4801 cggcgctggc gtaactcaac tcaccgattg atgcttcgcg ggtaacactg tgagcccgtc
     4861 cgcatgcctg ccgtgacacg ctgcctccat ctacgatgac ttcgtgcggc tcatcgccgc
     4921 ccacggcacc tgcaacgcag ggtggtcaat catcgtcgcc aggagaaatc atgacacagc
     4981 gtctcaacta ttttcagcaa tcgccggcac tggtcaagaa gttcattgaa ctcaacaacc
     5041 tgctgaaaga cggcaccatc gaagaatcaa tccgtgacct ggtctcaatc cgggcgtcgc
     5101 aactcaatgg ttgtggattt tgcctggaca tgcacgtgaa ggaagcgcat attcacggcg
     5161 agcgtgagct gcgcatccat cacctcgcgg cgtggcgcga gtcgacgctc ttcgcaccac
     5221 gtgaacgcgc cgcgcttgcc tggacggaag tgctgaccca ccttcccgaa cacggcgttc
     5281 ccgacgacat ctatgagcgc gtgcgtaccc agttctccga aaaggagctc tcggacctga
     5341 cgttcgacgt gatggccatc aacggatgga accgcgcgaa cgtggcgttc aagatggtgc
     5401 cgggttcgtt cgacaaggca tttggtctgg ataaggcgaa cctcgcctga gcggactatt
     5461 tcgcttctct tcaatcttgg ccctcgcggg cgggccgcgt cgctcaggag cacctatcat
     5521 gcttcgtttg aaaaactccg gtctggccgc gcttttcatc gtaggcgcgt
//
LOCUS       DATASH010001036         3731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5719286,
            whole genome shotgun sequence.
ACCESSION   DATASH010001036 DATASH010000000
VERSION     DATASH010001036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5719286"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..177)
                     /locus_tag="VHV99_27540"
     CDS             complement(<1..177)
                     /locus_tag="VHV99_27540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3639168.1"
                     /translation="MISIKNVSKWYGQFQVLTDCTTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPFQKGEIV"
     gene            complement(174..851)
                     /gene="gltK"
                     /locus_tag="VHV99_27545"
     CDS             complement(174..851)
                     /gene="gltK"
                     /locus_tag="VHV99_27545"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006025271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HEX3639169.1"
                     /translation="MHHFDWSGIPGALPTLWTGAIVTIKITLIAIVIGIIWGTILAMM
                     RLSPIKPLAWFAKGYVTVFRSIPLVMVLLWFFLIVPQVLQNVLGLSPDIDIRLASAMV
                     AFSLFEAAYYSEIIRAGIQSVPRGQVNAAFALGMSYPQAMRLIVLPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVISLADFFRTATNIGDRDGTNVEMVLFAGACYFVICVIASSLVKG
                     LQKKVAR"
     gene            complement(851..1591)
                     /locus_tag="VHV99_27550"
     CDS             complement(851..1591)
                     /locus_tag="VHV99_27550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015601464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX3639170.1"
                     /translation="MSYHWNWGILLSPVSTGEPTTYLGWLMSGFWVTITVSLSAWVIA
                     LIVGSFFGVLRTVPNRWASGIGTLYVAIFRNIPLIVQFFIWYLVIPELLPVSIGSWYK
                     QLPPGAQFFSSSIICLGLFTAARVCEQVRSGINALPRGQRAAGLAMGFTQWQTYRYVL
                     LPVAYRIIVPPLTSEFLNIFKNSAVASTIGLLDLSAQARQLVDYTSQTYESFIAVTLA
                     YVLINLVVMQLMRWVEARTRLPGYIGGK"
     gene            complement(1673..2566)
                     /locus_tag="VHV99_27555"
     CDS             complement(1673..2566)
                     /locus_tag="VHV99_27555"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX3639171.1"
                     /translation="MKVKKAALLLATLGLFTVGAHAQDAGTLKKIKDTGVISLGHRES
                     SIPFSYYDDKQNVIGYSQEFALKVVEAVKAKLNMPNLKVKLTPVTSQNRIPLVQNGTV
                     DIECGSTTNNAERQQQASFSNTIFVIGTRLMTKKDSGIKDFPDLKGKTVVTTAGTTSE
                     RLLRKMNQDKSMGMNIISAKDHGESFLTLSTGRAAAFMMDDALLAGERAKSNNPGDFV
                     IVGTPQSHEAYGCMIRKNDPEFKKVVDDAIAKVETSGEADQIYKKWFESPIPPKGLNL
                     NFPESDDIKALYKSPNDKAID"
     gene            complement(2934..3731)
                     /locus_tag="VHV99_27560"
                     /pseudo
     CDS             complement(2934..3731)
                     /locus_tag="VHV99_27560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014898081.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
BASE COUNT          792 a         1157 c         1042 g          740 t
ORIGIN      
        1 gacgatctcg cccttctgga acggctcgag gccgttgacg gtcttgatca gtgtggactt
       61 gcccgagccc gacggcccgc acaccaccac cacttcacct tttttgactt ccgtcgtgca
      121 gtcggtcagc acttgaaact ggccgtacca cttcgaaaca ttcttgatag agatcatctt
      181 gcgacctttt tctgaagacc tttgacgagg ctcgacgcga tcacgcagat cacgaaataa
      241 cacgcgcccg cgaacagtac catttcgaca ttcgtaccgt cacggtcgcc aatattcgtg
      301 gcggttcgga agaagtcggc gaggctgatc acgtagacga gcgacgtatc ctgaaacagc
      361 acgatgcctt gcgtaagcag cagcggcacc atcgcgcgga acgcctgcgg caatacgatg
      421 aggcgcatgg cctgcggata gctcatgccg agcgcaaacg cggcattgac ctgcccgcgc
      481 ggcaccgact gaataccggc gcggatgatt tccgaatagt acgcggcctc gaacagcgag
      541 aaagcgacca tcgccgaagc gaggcgaatg tcgatatccg gcgacaagcc cagcacgttc
      601 tgcagcacct gcggcacgat caggaagaac cacagcagca ccatcaccag cgggatcgag
      661 cggaacaccg tgacgtagcc cttcgcgaac catgcgagcg gcttgattgg cgaaagccgc
      721 atcatcgcga gaatggtgcc ccagatgatg ccgatcacga tcgcgatcaa cgtgatcttg
      781 atggtgacga tcgcgccggt ccacagcgta ggcagtgcgc ccggaatacc gctccagtcg
      841 aaatgatgca tcacttacct ccgatatagc cgggcaaccg ggtcctggct tcgacccagc
      901 gcatcagttg catcacgacc agattgatca gcacatacgc cagcgtgacc gcgataaacg
      961 actcatacgt ttgcgacgtg tagtcgacca gttggcgcgc ctgggccgac agatccagca
     1021 gaccgatggt cgaagcaacc gccgagttct tgaagatgtt cagaaactcg gacgtgagcg
     1081 gcggcacgat gatccggtac gcaaccggca gcaacacgta gcgatacgtc tgccattgcg
     1141 tgaaacccat tgccagaccc gcggcgcgct ggccgcgcgg caacgcgttg atacccgagc
     1201 gcacctgctc gcacacgcgc gcggcagtga acaacccgag acagatgatc gacgacgaga
     1261 agaactgcgc acccggtggc agttgcttgt accagctacc gatcgacaca ggcagcaatt
     1321 ccggtatcac cagataccag atgaagaact gcacgatcag cgggatgtta cggaaaatcg
     1381 caacatagag cgtgccgata cccgacgccc atctgttcgg cactgtgcgc agcacaccga
     1441 aaaacgaacc gacgatcagc gcgatcaccc atgccgacag cgacacggta atcgtcaccc
     1501 agaagccgga catcagccag cccagatagg tggtcggctc gccggtggag accggactca
     1561 gcaaaatgcc ccagttccag tgatatgaca tgaccaagac tccagcaaaa agaaacggaa
     1621 gaagcgtgtg gccccttccg tttcgttcag cgtttcaaaa aacagctagg gtttagtcga
     1681 ttgccttgtc attcgggctc ttgtagagcg ccttgatgtc gtcgctttcc gggaagttca
     1741 ggttcaggcc cttcggcgga atcggcgatt cgaaccactt cttgtagatc tgatcggctt
     1801 cgcccgaggt ttcgaccttc gcgatcgcgt cgtcaacaac cttcttgaat tccggatcgt
     1861 tcttgcgaat catgcagccg tacgcttcat gcgattgcgg cgtgccgacg atcacgaaat
     1921 cgcccgggtt gttcgacttg gcgcgttcgc ctgccagcag cgcgtcgtcc atcatgaacg
     1981 cagcagcacg gccggtggag agcgtcagga acgactcgcc atggtccttc gcgctgatga
     2041 tgttcatgcc catgctcttg tcctggttca tcttgcgaag caggcgctcg gaggtagtgc
     2101 cggccgtcgt gacgacggtc ttgcccttca ggtccgggaa gtccttgatg cccgagtctt
     2161 tcttggtcat cagacgcgtg ccgatcacga agatcgtgtt cgagaacgaa gcctgttgct
     2221 ggcgctcggc gttattcgtg gttgagccgc attcgatgtc gacggtgccg ttttgcacca
     2281 gcggaatacg gttttgcgac gtgacaggcg taagtttgac cttcaggtta ggcatgttca
     2341 gcttcgcctt gaccgcctcc accaccttca gcgcgaattc ctgcgagtag ccgatgacat
     2401 tctgcttgtc gtcgtaatac gaaaacggga tcgacgattc gcgatgaccc agcgaaatga
     2461 cgcccgtgtc cttgatcttt ttcagcgtcc cagcgtcttg cgcatgcgcg ccaaccgtaa
     2521 acagtccgag agtcgcgagc agcagcgcag cttttttaac cttcatgtgt gatctccttg
     2581 gcaagaaccg gcgccagttt agcaaagtaa ttattctgaa agtattgcta taaaccatgt
     2641 ctgttgcgtg tttcccaatc atccgacccc tcaagctctc aattcctcaa ggggacttcc
     2701 ttcggggcgg acgactttcc ccggaacgcg cgccactatg ggattacggc gccttagcca
     2761 ttgatccaaa cgccgcgcgc ccggaggaag tcgctttggg gcagacacgt gaaggcgggc
     2821 ggcatcgata cgatgccgcc cgccgggtca aacacgcggt ctcgtgaacc gcgttgaagc
     2881 aaggattagc tgcggtccgc gagttggcgg atccgccgtt tcagacaagc ggatcaggga
     2941 tacaggccac gcagttcgcg cgcttcgaga atccgcttac atgcgacgat aaacgcagca
     3001 gtccgcacgg acactttctg ttcgctcgac acttgccaca ctgcggcaaa tgcttcacgc
     3061 atcacgcgtt cgagacgctg gttgatttcg tcttcggtcc agaagaagct cgagaagtcc
     3121 tacacccact cgaagtacga caccgtcacg ccgccggcat tcgccaccac gtccggaatc
     3181 acgaggatgc cgcggtcgtg cagaatgtcg tccgctgccg tggtggtcgg gccgttggcg
     3241 ccttccacga cgatcttcgt cctgatcttt cccgcgttct tttcggtgat ctggttttcc
     3301 agcgcagccg gaatcagaat gtcggattcg acggtccaga attcctcgtt cgtcaccgcg
     3361 tcggcttccg ggaaaccgcc cacgccgccc gtcttcgcga cgtgctcgag caatgcgccc
     3421 gcatcgatac cggtcgactt gtacagcgaa ccggtgtgat cctgcaccgc gaccaccttc
     3481 gcgcccgctt cctggaacag acgcgcagca ataccaccca cgttaccgaa gccctgcacg
     3541 gcgatccgcg cgccctggat gtcgaagccg atgcggcgtg ccgcctcgct accgaccacg
     3601 aacacgccac ggccggtcgc ttcacggcgg cccagcgaac cgccgagcgt aatcggcttg
     3661 ccggtcacga cgccggtggc cgtttggccc tggttcatcg agtacgtgtc catcatccac
     3721 gccatgatct g
//
LOCUS       DATASH010001037         2930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2552209,
            whole genome shotgun sequence.
ACCESSION   DATASH010001037 DATASH010000000
VERSION     DATASH010001037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2552209"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..1053)
                     /locus_tag="VHV99_27565"
     CDS             complement(37..1053)
                     /locus_tag="VHV99_27565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HEX3639172.1"
                     /translation="MKIGIANDLPLAVEALRRALAARQDYEVLWVAQDGQQAVDFCAA
                     QRPDIVLMDLVMPNVDGIEATRRIMAKAPCAILIVTVDVGANAWRVYEAMGAGALDAV
                     DTPSLSGPDAHKSIATLIAKIDHVAALVKARNVPGAAAPAGPAGVTNRDTPLVAIGAS
                     AGGPAALATLVAGLPKDFPAAIVIVQHVDAAFAAGMADWLNQQSALPVRIAIEGDRPQ
                     AGVALLAATDDHLHLKLPDVLGYTRVPEETPYRPSVDVFFQSVVARWPARAIGVLLTG
                     MGRDGAIGLKAMRTKGYHTIAQDEATSAVYGMPKAAAALNAAASILPLPRIAAALAAA
                     VNAR"
     gene            complement(1071..>2930)
                     /locus_tag="VHV99_27570"
     CDS             complement(1071..>2930)
                     /locus_tag="VHV99_27570"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEX3639173.1"
                     /translation="LANALRAEASPASAAPASRVASASESTSNEASPPATDPGRMLRV
                     RADNLDRLLSLSGESLVESRWLKPFAQSMLRVKRVQRDGSRALDQLHETLADLRLDPR
                     AHAALEELRRLTAESQHLLAERLADLESFDRRSTHLSQQLYDAALQCRMRPFGDGTSG
                     LARMVRDVARSLGKKVRWQLVGESTQVDRDILDLLEAPLGHLLRNAIDHGIEAPAHRL
                     ALGKSEEGTLTLDARHTAGALVITVTDDGAGIDLDRLRTSIVRKKLASAETAARLSEI
                     ELLEFLFLPGFSLRDQVTEVSGRGVGLDAVHDVVKRVRGTVRITHEPGVGTRVQLQLP
                     LTLSVIRSLLVEVAGEPYAVPLAHVNRTLHVRRPEIELLEGHQHIAFDGRRIGVVTAH
                     QILDTAPPANEADTVSLIVIGDGAQTFGVVVDRFLGERMLVVHPLDPRLGKIRNITAG
                     ALMENGDPVLIADVDDWLRSVEKLVAGGDLQHTQLGAALTAQRATRRVLVVDDSLTVR
                     ELERKLLATRGYDVTIAVDGMDGWNAVRGERFDLVITDIDMPRMDGIELVTLIKRDPQ
                     LQALPVMIVSYKDREEDRRAGLNAGADYYLAKGSFHDEALLDAVRDLIGEAYG"
BASE COUNT          438 a          951 c         1033 g          508 t
ORIGIN      
        1 agtgaatcgg ctcgtcggtg ttggccaggg tgcgttctat cgtgcattca ccgccgcggc
       61 gagcgcggcg gcaatccgtg gcaacggcag gatcgaggcg gcggcgttga gcgcggcggc
      121 cgccttcggc atgccgtaca ccgcgctggt cgcctcgtcc tgcgcgatcg tgtgatagcc
      181 cttggtgcgc atcgccttga ggccgatcgc gccgtcgcgg cccattccgg tcagcagcac
      241 accaattgcg cgcgccggcc agcgtgccac cacgctctga aaaaagacgt cgacggatgg
      301 gcggtacggc gtttcttccg gcacgcgcgt gtagccgagc acgtcgggca gtttcagatg
      361 cagatgatcg tccgtggcgg ccagcagcgc gacgcccgct tgcggccgat cgccttcgat
      421 ggcgatccgc acgggcagcg ccgactgctg gttcagccaa tcggccatgc cggcggcgaa
      481 tgccgcgtcg acgtgctgga cgatcacgat ggccgccggg aaatccttcg gcaagccggc
      541 aaccagcgtg gcgagcgccg ccgggccgcc cgccgaagcg ccgatcgcaa ccagcggcgt
      601 gtcgcggttc gtcacgccgg ccggtcccgc cggcgccgcg gcgccaggca cattgcgcgc
      661 cttgaccagc gcggcgacgt ggtcgatctt cgcgatcagc gtggcgatgc ttttgtgcgc
      721 gtccggaccg ctcagcgagg gcgtatccac cgcatcgagc gcgcccgcgc ccatcgcttc
      781 gtagacgcgc cacgcgttcg cgcccacgtc gacggtcacg atcaggatcg cgcacggcgc
      841 cttcgccatg atgcggcggg tggcctcgat cccgtcgacg ttcggcatca cgagatccat
      901 cagcacgatg tcgggccgct gcgcggcgca gaagtcgact gcctgttggc cgtcctgcgc
      961 aacccacagc acctcgtagt cctggcgcgc ggcaagcgcg cggcgcagcg cctcgacggc
     1021 gagcggcagg tcgttggcga ttccgatttt cattcgtttt gcccgtaggt ttaaccgtaa
     1081 gcttcgccga tcagatcgcg cactgcatcg agcagtgctt cgtcgtggaa actgcctttc
     1141 gccagataat aatccgcgcc cgcgttcagg ccggcgcggc ggtcttcttc acgatccttg
     1201 tacgagacga tcatcaccgg cagcgcctgc agttgcggat cgcgcttgat gagcgtgacg
     1261 agttcgatgc cgtccatgcg cggcatgtcg atgtcggtga tcacgaggtc gaagcgctcg
     1321 ccgcgcaccg cgttccagcc gtccatgccg tcgacggcga tcgtcacgtc gtagccgcgc
     1381 gtcgccagca gcttgcgttc gagttcgcgc acggtgagcg aatcgtcgac caccagcacg
     1441 cgccgtgtcg cgcgctgtgc cgtgagcgcg gcgccgagct gcgtgtgctg cagatcgccg
     1501 cccgcgacga gcttttcgac cgagcgcagc cagtcgtcga cgtccgcaat cagcacgggg
     1561 tcgccgtttt ccatcaacgc gccggctgtg atgttcctga tcttgccgag gcgcgggtcg
     1621 agcgggtgca ccaccagcat ccgttcgccg agaaagcgat cgaccaccac gccgaaggtt
     1681 tgtgcgccgt cgccgatcac gatcaggctg acggtatcgg cttcgttggc gggcggcgcg
     1741 gtgtcgagaa tctgatgcgc ggtgacgacg ccgatgcggc ggccgtcgaa ggcaatgtgc
     1801 tgatggcctt cgagcagttc gatctccggg cggcgcacgt gcagcgtgcg gttcacatgc
     1861 gcgagcggca cggcgtacgg ctcgcccgcg acttcgacga gcaggctgcg aatcaccgag
     1921 agcgtgagcg gcagttgcaa ctgcacgcgc gtgccgacgc cgggttcgtg cgtgatgcgc
     1981 accgtgcctc gcacacgctt gacgacgtcg tgcaccgcgt cgagcccgac gccacggccc
     2041 gagacctcgg tgacctggtc gcgcaacgag aagccgggca ggaacaggaa ttccagcagt
     2101 tcgatttcgg acaggcgagc ggctgtttcg gcgctcgcca gttttttgcg gacaatcgag
     2161 gtgcgtagcc ggtcgaggtc gatacccgcg ccgtcgtcgg tgacggtgat gaccagcgcg
     2221 ccggcggtgt ggcgcgcgtc gagcgtgagc gtgccctctt cggacttgcc gagcgcgaga
     2281 cggtgcgcgg gcgcttcgat gccgtggtcg atcgcattgc gcagcaggtg gccgagcggc
     2341 gcttcgagca gatcgaggat gtcgcggtcg acctgagtcg attcgccgac cagttgccag
     2401 cgcaccttct tgccgagcga acgcgcgacg tcgcgcacca tgcgcgcgag accgctggtg
     2461 ccgtcgccga aggggcgcat ccggcattgc agtgcggcgt cgtaaagctg ctgcgacaga
     2521 tgcgtcgagc ggcggtcgaa gctctccaga tcggcaagac gctcggccag cagatgttgc
     2581 gattccgcgg tgaggcggcg caattcttcg agcgcggcgt gcgcgcgcgg atcgagtctc
     2641 agatcggcga gcgtttcgtg caactgatcc agcgcgcgcg agccgtcacg ttggacgcgt
     2701 ttgacgcgca gcatggattg cgcgaagggt ttgagccagc gcgattcgac cagcgattcg
     2761 ccggataggg agaggaggcg gtcgaggttg tctgcgcgta cgcgcagcat gcggccgggg
     2821 tctgttgctg gggggcttgc ttcgttcgag gtggattcgg aggcacttgc aaccctgctc
     2881 gccggtgcgg ctgaggcggg ggacgcttct gcgcgcagcg cgttggctag
//
LOCUS       DATASH010001038         2448 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3345016,
            whole genome shotgun sequence.
ACCESSION   DATASH010001038 DATASH010000000
VERSION     DATASH010001038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3345016"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1661)
                     /locus_tag="VHV99_27575"
     CDS             complement(<1..1661)
                     /locus_tag="VHV99_27575"
                     /inference="COORDINATES: protein motif:HMM:NF024806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing
                     glycosyltransferase family protein"
                     /protein_id="HEX3639174.1"
                     /translation="MCADLHTGQEIDAYADAHASAPALLRLAAILDTQCRHADAAVLL
                     KAAEIAAPALPGLQARLALSLSAAGEHAAARKQFEAVLGGDASAHTASVDNPPVASGL
                     QLAYSHTLLVLDAPEEALAQAENAVAGNPDDACAHAARGDALAVLLRLPDALTAYQRA
                     ARLEPSLAWAHYGIGKVFIELGRPMAALEGLSLALALSENIEQDADAGAQALTPPAPL
                     SFPCLPIHALSPSAICESIGLAWAKGCQLAQALPWFERALELEPNRATALRHMGNTLA
                     MMRADDAALACLQAARRVCPDWDKAWLDEAGLLLRRGDYAAGWRAYGKREGQRLLESL
                     PSCWSGEESLADKTILLLAEQGLGDTIQFARYAPQVAALAQRVILEVHAPLRPLFDEV
                     ARDWGVEIVARGDARPDGDHQCLLLSLPRILRTTPDSIREPSRYLRAPEARRALWRER
                     LAARSATGRLRVGLAPAGNPQFKNDALRSIPLAAFEACFDLPGVDWVITQPEIRDADR
                     AVLERHPGVISFGAQLEDFADTAALLEQLDLVISVDTSIAHLAGAL"
     gene            2238..>2448
                     /locus_tag="VHV99_27580"
     CDS             2238..>2448
                     /locus_tag="VHV99_27580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015877991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HEX3639175.1"
                     /translation="MTTVINTNIASQIAQNNLLNNQAAVTSAITQLSSGLAINSAADN
                     PAGLAIATTLQAQINGQTVAQQNANN"
BASE COUNT          499 a          776 c          776 g          397 t
ORIGIN      
        1 agtgccccgg ccagatgggc gatcgacgtg tcgaccgaga tcaccaggtc gagttgttcg
       61 agcagcgccg ccgtatcggc aaagtcttcc aattgggcac cgaacgagat gacgccggga
      121 tgccgctcca acaccgcgcg atccgcgtcg cggatctcgg gctgcgtgat cacccagtca
      181 acgcccggca agtcgaagca ggcttcgaaa gcggcgagcg gaattgagcg cagtgcgtcg
      241 ttcttgaact gcggattgcc ggccggtgcg agaccaaccc gcaatcgtcc cgtcgcgctg
      301 cgagccgcga gccgctcgcg ccagagcgct cgccgcgcct cgggtgcgcg aagatacctg
      361 ctcggctcac gaatgctgtc aggcgtcgtc cggagtatgc gcggaagact gagcagcaag
      421 cattgatggt cgccatcggg tcgcgcgtcg ccgcgtgcaa cgatctcgac accccagtcc
      481 cgggccacct cgtcgaacaa cggccgcagc ggcgcgtgaa cctcgaggat cacgcgttgc
      541 gcgagagccg ccacctgcgg cgcataacgc gcgaactgga tcgtgtcgcc gaggccctgt
      601 tcggcaagca gaaggatcgt cttatcggcg agcgactctt cgccggacca acacgacggc
      661 agactttcga gcagccgctg tccttcgcgc ttgccatacg cgcgccagcc tgcggcgtag
      721 tcgcctcgac gcagcagcag gcccgcctcg tcgagccagg ccttatccca gtcaggacat
      781 acgcgacggg ccgcctgaag gcacgccagc gcagcgtcgt cggcacgcat catggccagc
      841 gtgttaccca tatgccgcag agcggtcgct cggtttggct cgagttccag cgcacgttcg
      901 aaccagggca gtgcttgcgc gagctggcag cctttggccc atgccagtcc gatcgactca
      961 cagatagcgg atggggataa agcgtggatc ggcaaacacg gaaagctgag cggcgccggc
     1021 ggtgtgagag cctgcgcccc tgcatcagcg tcctgctcga tgttttccga aagcgccaag
     1081 gcgagcgaca gcccctccaa cgcggccatg ggccggccga gttcaataaa tactttgccg
     1141 atgccatagt gcgcccatgc caatgacggt tcgagcctgg ccgcgcgttg gtaggcggtg
     1201 agcgcgtccg gcaaccgcag cagcaccgcc aatgcgtcgc cgcgcgcggc gtgagcgcat
     1261 gcgtcgtcgg gattgcccgc aaccgcattt tccgcttgcg ccagcgcttc ttccggcgcg
     1321 tcgagcacga ggagcgtgtg cgaataggca agttggagtc ccgacgctac cgggggattg
     1381 tccacactgg ccgtatgggc cgatgcatcg ccccccagaa cggcttcaaa ctgcttacgc
     1441 gctgcggcat gttcgccagc ggccgaaagc gacagcgcga gtcgcgcctg caagccgggc
     1501 aacgccggtg cagcaatctc tgcagccttt aaaagcacgg cggcgtccgc atggcggcat
     1561 tgagtatcaa ggatcgcggc gagccgcagc aatgcgggcg cgctcgcgtg cgcatcggcg
     1621 tatgcgtcga tttcctgacc ggtatgcaga tcggcgcaca tttgacaaag cgggtccgtc
     1681 attagcgtaa ccaagggtgt tgacaagcga gtgccgtcat tctgaatcag gtcgacgggc
     1741 aacaaaggtc ggaacagcgg cttaagttag cgccaacttg cggaatcgcc acttgctgcg
     1801 tcgcagcatc gaatggtcca cctgcgccgg tgcagcaggg gctgaacgag gcgattaggc
     1861 ggcgggacgg cgtctccgtg cagatgcaag acgcaatgtc aaggcgaccg acagggcctc
     1921 gcgcgaagca agccgatccg gcaaccgcag cctcgcgccc taccgcagca cccgacgcga
     1981 ctggcagcga gtggacctga tggtgcggtc aggccgttcc gctggctggc gagactgacg
     2041 atggaccggc ctcgcgtaag aatccagcgc gttcagaaac gcaaaaggtt gcggcattcg
     2101 cgagatacca gcattaaaga tttccagaac ctatccgata acccacttaa cgacggcaaa
     2161 tggacatcgc attacacggc cgtacgttac ggcgaacttg ccggaatgtg caaaccatta
     2221 gatataggag tagttgaatg actacggtta tcaataccaa cattgcgtcg cagattgcgc
     2281 agaacaattt gctgaacaac caggccgcgg tgaccagcgc gatcacccag ttgtcgtcgg
     2341 gtctcgcgat caacagtgca gcggataacc ctgcaggcct ggctatcgcg accacgctgc
     2401 aagcgcagat caacggccag accgttgcgc agcagaacgc gaacaacg
//
LOCUS       DATASH010001039         5174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3936919,
            whole genome shotgun sequence.
ACCESSION   DATASH010001039 DATASH010000000
VERSION     DATASH010001039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5174
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3936919"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="VHV99_27585"
     CDS             complement(<1..442)
                     /locus_tag="VHV99_27585"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX3639176.1"
                     /translation="MVSPLGNSSETFFVNLARGRSGIVALPAPATAPPGRSVLRDPRN
                     HIGGVVAFDGARYFPAPKLRMLDRVSQFALVAADQAVRDAGLLFDGPGCERAGVSVGT
                     GMGGAQSADAGYFTLYAEGSDRVKPYSVLMAMTNAAASWIGIEYG"
     gene            complement(469..747)
                     /locus_tag="VHV99_27590"
     CDS             complement(469..747)
                     /locus_tag="VHV99_27590"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEX3639177.1"
                     /translation="MTTYESMQTILQAKFALAPDVLQPDTVLKELDVDSLSMVEILFE
                     VEDELKLEIPSEPAEWQSQMKTFGDLVEYVDKLVAAQRPVTASAEMPA"
     gene            complement(811..2064)
                     /locus_tag="VHV99_27595"
     CDS             complement(811..2064)
                     /locus_tag="VHV99_27595"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX3639178.1"
                     /translation="MHERYDAIVVGGGPSGATAAALLARAGWRIGVVEKALFPRRKVC
                     GEFISATTWPLLGELGVSGQLLKIAGPPVRRVGVYAGSVMVTAKLASTVGFARSGEGR
                     AVGREHLDTLLLQRAAIAGATIWQPWLLEQFVAHDGGYDCTIVNKATRESRVLQSRLI
                     IAAHGSWESGPLPTQDFRRPSSPADLFGFKAHFTGSTLPQDLMPLLAFPGGYGGMVHT
                     DAGRISLSCCVRRDQLERCRRRAPDVRAGEAVLSHIASSCKAVSLALIGTRSDGVWLS
                     AGPLRTGIRTFGRDGIFAVGNAAAETHPIVAEGISIAIQSAALLCEQLIARPELMRDG
                     AGTVAAFEAARADYAAAWRANFSPRLFVAAVFASVFTRPIATRIATTLLERFPQLLAE
                     GARWSGKVQPLRSMSTSHGCHRNQH"
     gene            complement(2065..2739)
                     /locus_tag="VHV99_27600"
     CDS             complement(2065..2739)
                     /locus_tag="VHV99_27600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639179.1"
                     /translation="MLDGLPAEDPRSQRSRRDLRRIQKAMATLPIVQRALDRGTSSFV
                     PRTLLELGAGDGSLMLRLARRRAACWPDVGVMLLDRVNLVAPQTLDGMREVGWTPRVM
                     VTDVFEWLAKRDDARWDVVFANLFVHHFSSEALEKLLVGIAARTNVFLCCEPRRSVLA
                     LTGSHLIGLLGAGLVTRHDAVSSVHAGFRAQELSSLWPDQHNWAIHEYPAGLFSHIFL
                     AVRRVR"
     gene            complement(2798..3853)
                     /locus_tag="VHV99_27605"
     CDS             complement(2798..3853)
                     /locus_tag="VHV99_27605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HEX3639180.1"
                     /translation="MFVTGLGTANPPQRYSKAECLEAFKASAWFLKLDRRAHIIAETV
                     LTKDNGIAYRTLSVDSLSEVFQVDPDTLHARFLANAPVLAAAAGKSALEDARLTASEI
                     DGLVVSTCTGYLCPGLTSYVVERLGLRADVLAFDLVGQGCAAALPNWRLADALLTAGC
                     RHVVSICVEVSSAAMYLDNDPGVLISACLFGDGAGAAVLSRDAPPRRRSIEWKSFASL
                     VNPAERSALMFATRKGMLRNILTRPVPGLAAEYAQQVLHTVLDSAGLAPADIGTWIMH
                     AGGRDVLDALQRRLKLEAHDLRYSADMLRQYGNMSSAFVYFVLQAALKDDAKPGWWWM
                     SAFGAGFSCHGALLEAA"
     gene            4023..4826
                     /locus_tag="VHV99_27610"
     CDS             4023..4826
                     /locus_tag="VHV99_27610"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HEX3639181.1"
                     /translation="MSQPNGNLGSTERWISALLGAGFTLAAIGGGGPLRRLAAAAVGA
                     SLFTRSATGYCAVKGAMTGDTSLANGLKEQWNRIRVQSEATAEGTADIDSLHTLYVSE
                     LQELRSAETQLQSLLRSLPGSFQSSSLDQVLRAYATDIEARTSGLDALIASMGANPRA
                     HSDRAMRALIRETRQMTRVRSAHVRGAALVASLQRLIHFKIAGYGTAATYATMLPRPD
                     DAQLLHGYVERDKEIDNELTALAKAIVNPEALAPEQPSPSEPSMAATPH"
     gene            complement(4995..>5174)
                     /locus_tag="VHV99_27615"
     CDS             complement(4995..>5174)
                     /locus_tag="VHV99_27615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639182.1"
                     /translation="QAVQAAKNAADASASVASKTSFAVFGVMLLGAIAAALGGSMATQ
                     GQILVRSRRTTARPT"
BASE COUNT         1076 a         1667 c         1529 g          902 t
ORIGIN      
        1 acccgtactc gataccgatc caggacgcgg ccgcattggt catggccatc aacacgctgt
       61 agggtttgac gcgatcggag ccttccgcgt aaagggtgaa gtagccagcg tccgccgatt
      121 gtgccccccc catgccggtg ccgacagaaa ctcccgcgcg ttcgcacccg ggaccatcga
      181 acagcagacc cgcatcgcgc accgcttggt cggcggctac cagcgcgaac tggctgaccc
      241 gatccagcat gcggagcttc ggagcgggaa agtagcgtgc accgtcaaac gcgaccacgc
      301 ctccgatgtg attccgcgga tctcgaagga cggaccggcc gggcggcgcg gttgccggcg
      361 caggcaatgc gacgattccc gagcggccac gcgcgagatt gacgaagaat gtttccgagc
      421 tgtttcccag cggagaaacc acaccgattc ctgtgatcgc tacgcgtctc aagccggcat
      481 ttccgcgctg gctgtcaccg gccgctgtgc ggcaacgagt ttgtcgacat actccacgag
      541 gtcgccgaag gttttcatct ggctttgcca ctcggcgggc tcggacggga tctcgagctt
      601 gagttcatcc tcgacttcga acaggatttc gaccatggac aatgaatcaa cgtcgagctc
      661 tttcaaaact gtatcgggtt gcagaacgtc gggcgccaaa gcgaatttgg cttgcaagat
      721 ggtttgcatc gattcatacg tcgtcatcgc gcattctcca cgaaatgtcg gtcaatcatt
      781 cgggcacgga aaggggcgag gcgatccctc ttagtgttga ttacgatggc acccgtggga
      841 ggtgctcatg ctgcgcaacg gctgtacttt gccgctccag cgagcgcctt cggcaaggag
      901 ttgcgggaag cgctccagca atgtggttgc gatacgcgtg gcaataggcc gcgtgaacac
      961 gcttgcgaat acggcagcca cgaagaggcg cggcgaaaag ttggcgcgcc acgcagccgc
     1021 gtagtcagcc cgggcagcct caaacgcggc gacggtaccg gcgccatcgc gcatcaactc
     1081 gggtcgtgca atcagttgct cgcacaacaa ggccgccgac tgaatggcaa tgctgatccc
     1141 ctcggcgacg atgggatgcg tttcggcggc cgcgttaccg accgcgaaga tgccgtcgcg
     1201 gccgaaagta cggatcccgg tccgcagcgg tccagccgac agccagacgc cgtctgacct
     1261 cgtcccaatc aacgccagtg aaactgcctt gcatgacgac gcgatatgcg acagaaccgc
     1321 ttcacctgct cggacatcag gtgcgcgtcg ccggcaacgt tcgagctggt cgcgacgaac
     1381 gcagcaggac aggctgatac gacctgcgtc agtgtgcacc atgccaccat agccgcccgg
     1441 gaaagcaagc aatggcatca gatcctgtgg cagtgtgcta cccgtgaaat gagccttaaa
     1501 accgaacaga tccgcgggac tcgacggccg gcggaagtcc tgcgtcggca gcgggccaga
     1561 ctcccacgaa ccatgcgcgg caataatgag ccgcgattgc aatacacggg attcccgggt
     1621 ggccttgttt acgatggtgc aatcgtagcc tccatcgtgc gcgacgaatt gctcgagcaa
     1681 ccatggctgc catatcgttg ccccggcgat cgcggcgcgc tgaagcagca gagtatcgag
     1741 gtgctcgcgc cctaccgccc tgccctcgcc gctccgggca aaaccaaccg ttgaagccaa
     1801 tttagctgtc accatgacac tgccagcgta gacaccgacg cggcgcaccg gaggtccggc
     1861 aattttcagc aactgaccgg aaacgcctaa ctccccaagc aagggccagg tcgttgccga
     1921 gataaactcc ccgcacactt tgcgtcgcgg gaacaacgct ttctctacga caccgattcg
     1981 ccagcctgcg cgcgccaaga gcgcggcggc cgtagcccct gagggaccac cgcccacaac
     2041 gatcgcgtcg tacctttcgt gcatctagcg caccctccgg acggcaagga aaatgtggct
     2101 gaacagcccc gcgggatact cgtggatcgc ccaattgtgt tgatcaggcc agaggctcga
     2161 caactcttgc gcacggaacc ctgcgtgcac actcgatacc gcatcatgtc gggtcaccag
     2221 accggctccc aacagcccga tcaggtggct cccggtaagt gccaggacgg aacgtcgcgg
     2281 ctcgcagcaa agaaacacgt tagtgcgcgc ggcgattccc acaaggagct tctccagcgc
     2341 ctctgacgaa aagtgatgca cgaacagatt ggcgaagacg acatcccacc gcgcgtcgtc
     2401 gcgcttcgcc aaccattcga agacatccgt caccataacg cgcggtgtcc agccgacttc
     2461 gcgcatacca tcaagggtct gaggggcaac caggttcacc cgatccagaa gcatgacgcc
     2521 gacgtcgggc caacacgcag ctcttcgtcg cgccagtcgc aacatcagtg agccgtctcc
     2581 ggcgccgagc tcaagcaacg tgcgcggaac gaaactcgac gtgccacgat ccagggcacg
     2641 ttgcacgatc ggcagtgtcg ccatcgcctt ctggatgcgc cgtaggtcgc gcctcgagcg
     2701 ttgcgagcgc ggatcttcgg cggggaggcc gtcaagcatt tcgcactcca ctcgccgtgg
     2761 caggcttagt gtcatgacct gtctcgccac tttcgtttca cgccgcctcc agcagtgctc
     2821 catggcaact gaaccctgct ccaaacgcag acatccacca ccagccgggt ttagcgtcat
     2881 ctttcagcgc cgcctgcagc acgaagtaca caaaggcgct gctcatgttg ccgtactgac
     2941 gcagcatgtc ggcgctgtag cgaagatcgt gcgcctcgag ttttagccga cgttgaagtg
     3001 cgtccagcac gtcgcggccg ccggcgtgca tgatccaggt tccgatgtcc gccggcgcca
     3061 agccagcgct atcgagcacc gtgtgcagta cctgctgcgc atactcggca gcaaggcccg
     3121 ggactggacg ggtcaggata ttgcgcagca tacccttacg agttgcaaac atcagcgcac
     3181 tgcgctcggc cggattaacc agtgaggcga atgacttcca ttcgatgctc cgacgccgcg
     3241 gcggtgcatc ccgggacaac acggcggctc cagcaccgtc gccaaataag cacgcgctga
     3301 tcaagacgcc tggatcattg tcgagataca ttgcggcact gctgacttcg acgcagatgg
     3361 acacaacgtg tcggcacccg gccgtcagca atgcgtccgc cagccgccaa ttcggcagcg
     3421 ccgctgcaca gccttgtccg accagatcga aagccagaac gtccgcgcgc aggcccaggc
     3481 gctcgacgac gtaactggtc aacccggggc agaggtaacc cgtgcaggta ctgacaacga
     3541 gtccgtcaat ttcagatgcg gtgagtctcg cgtcctccag cgcggatttg ccggcggcgg
     3601 cggccaggac cggggcattg gccaaaaacc gggcatgcag cgtatcggga tcaacctgaa
     3661 agacttcact cagggagtct acagacagcg ttctgtaggc gatcccattg tctttggtca
     3721 ggaccgtttc ggcaatgatg tgcgcgcggc gatccagttt gaggaaccac gcggaagctt
     3781 tgaaggcctc aaggcattcg gctttgctgt agcgttgcgg tggattagcc gtgccgagac
     3841 cagtaacgaa catccagact ccgattgtgt ccatgccgac gattcacgcc ggcttcggta
     3901 aagcatccaa gggcagactc gcggtagcgg cgaccctgcg ggccaacgcg aaatgcggaa
     3961 cgcatattgc ttctcttcta ccacaatcat tcactcgacg aacagcgcac cggagacggt
     4021 cgatgtcaca acctaacgga aacttaggat ccactgagcg gtggatctcg gcgctcctgg
     4081 gagcgggttt tactcttgca gcgatcgggg gcggcggtcc cctgcgacgc cttgccgccg
     4141 cagcggtcgg agcgtcgctg ttcacccgaa gcgcaaccgg ttactgcgct gtgaaaggcg
     4201 cgatgacggg agacacctcg ctggccaacg ggctcaagga gcaatggaac cgaatcagag
     4261 tgcaaagtga ggcaacagca gaaggaactg ccgacatcga cagcctccat accttgtatg
     4321 tcagcgagct gcaggaactc cgcagcgctg aaacacagct acagtcccta cttcggtccc
     4381 tgcccgggag ttttcagagc agctcactcg atcaggtgct gcgtgcctac gcgactgaca
     4441 tcgaagcccg gacttcgggt ctagacgcgc ttattgcgag catgggcgcc aacccacgcg
     4501 cgcattccga ccgggcgatg cgcgcgctga tccgcgagac gcgacaaatg acccgggtac
     4561 gctctgctca cgtgcggggt gccgcgctcg tggcctcatt acaacgtctc attcacttca
     4621 aaatcgcagg ctacggcaca gcagccactt acgcgacaat gctcccgcga cccgacgatg
     4681 cgcagctcct tcacggatat gtcgagcgtg acaaagagat cgacaacgaa ctcaccgcgc
     4741 tggcgaaagc gatcgtgaac ccagaggccc tggcgccgga gcagccttct ccgtccgagc
     4801 cctctatggc ggcaaccccg cattaggtca tcgcctgtca ggtgcgactg gcgctagccc
     4861 gtgttccgct ggtcgttgtg gcgatggctg tggcaatcgc acttacgcat gctctctcgc
     4921 ttaacgaggg gcgcgaccgc gtggcggctg gggctacacc gtcgcgcgga ccgtcgagga
     4981 ctatgagcgc ccggttatgt aggccgagct gttgttcgtc tactccgcac gaggatctgc
     5041 ccctgggtcg ccatggaacc tcccaaagcc gccgcaatcg cacctaacag catcacacca
     5101 aacaccgcga acgaggtctt ggaggcaacc gatgcactgg catccgccgc attcttagcg
     5161 gcctgaaccg cctg
//
LOCUS       DATASH010001040         3810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4730534,
            whole genome shotgun sequence.
ACCESSION   DATASH010001040 DATASH010000000
VERSION     DATASH010001040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4730534"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3810
                     /locus_tag="VHV99_27620"
     CDS             <1..>3810
                     /locus_tag="VHV99_27620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008351479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEX3639183.1"
                     /translation="IAWRTLLGPIPVLANLTVDAPDVVISRAADGTFSVAGVPIQAKH
                     TGNDAFMTWLLRQQAIVLRDGTLRWHDGERDAPELALKRIKLAILNDGTHHRVALQAP
                     PDGDLLRGPLDFRADFRTPHFSVPGRPANWTGHAYLSTGPVDLPMLAQYVEMPFATYS
                     GRFDNRIWIDFASGKLQTAGGDLTGSEIALRVRPTQPRLDVPIASFRWNLAQKEDEYT
                     LHLANLHAELGQPPLADGTPVSRLLALHTLTGNYRAATLQRGQLFGVAGDVVDLGILG
                     EFARALPLPSKAQNDLIRFNPRGQIANYTLQTERPRPATHAQMLAQRQTGAEPPMRYT
                     VKGDLRGVSVQAQEPPPGLTINNHPRAGIPGVENLWGSIDADQDHGSAVIDTKNTAIT
                     LPGVFDNPRLMLDQIDGRVTWTLAEAPGKPHKAITAHVETLHVRNADAEASATADYRN
                     EGEGRGSLDLKARAARLNVTSLVHYLPTSISEKLRLYLGHALQAGISRNGTIEIHGDL
                     TKFPYSRFPDAGQFRIVAPFTGGKFEPTPYPPRTMANGTPNVWPAFDGIDGTFHLAEN
                     KLGFDISRGRYLGVSVVKTTGRIEDLGNRESKLVIDADAHGPVTDMLHYVEDSSLGAL
                     SHHVSTKIHATGTAALALQLEIPRVAPPPGVRPHVGYKGALTFNGNELAYGALPPLSN
                     LKGRTRFGEKTASLDGLTAKFLGGDVRGTGGVKPDGSYAFNINGRIGGDAVQSLNAHD
                     QAQAAQILKHISGTAPYALRVRGVKNALPQVEANSDLTGLGLDFPAPFNKSEGTPMPV
                     SFTFTPVAGSPQEHDATLTFGPVNAHYLLKQTAKEMPIVERGAIGVNKPADLPAQGVT
                     AAVDIDELDADAWRKLIAELGPLPQPDPKMQSQVVKQFIPKRFALHFATIKLLNRRWE
                     DVAIGANLGDDRKWQANIASDQVTGNAEWMPGATKGSPGALQARFTKVVIPGKEETDL
                     VGQMIRKPPANMPSIDLIVNELVVRGRTLGRLEVDAHNDVENKVPVWYLDKLDLSNPD
                     ATLSATANWRTISGPVGFPANADDNAPRRTALDFKLDITDAGALVERLGLPRTVKNGK
                     GTLTGQLGWDGSPAAVDLPTLNGRLNVDLHHGQILKVNPGAAKLLGVLSLQSLAHFLT
                     LNFRDVVGKGLPFEKITGSGTIQNGIGRTNDFTMITAPARVELKGLVNLPKETQDLHA
                     KVIPTISAGAVALGAAVINPLLGLGTLVADLVLSKSIGKAFTIDYSITGSWSKPVIQR
                     VKGDQGK"
BASE COUNT          758 a         1208 c         1198 g          646 t
ORIGIN      
        1 catcgcgtgg cgcacgctgc tgggtccgat ccccgtgctc gcgaatctca ccgtcgatgc
       61 acccgatgta gtcatctcgc gcgccgccga cggaaccttc agtgtggccg gcgtgccgat
      121 ccaggcgaaa cacacgggca acgacgcgtt catgacctgg ctgctgagac agcaagccat
      181 cgtgctgcgc gacggcacgc tgcgctggca cgacggcgaa cgcgacgcgc cggaactcgc
      241 gctcaagcgc atcaagctcg caattctcaa cgacggcacg catcatcgag tcgcgttgca
      301 ggcgcctccc gacggcgacc tgctgcgcgg accgctcgat tttcgcgcgg acttccgcac
      361 gccgcacttc agtgtgcccg gccggccggc aaactggacg ggccacgcgt atctgtcgac
      421 cggtcctgtt gatctgccca tgctcgcgca gtacgtggag atgccgttcg cgacctacag
      481 cggacgcttc gataaccgca tctggattga cttcgcgagc ggcaagctgc aaacagcggg
      541 cggcgacctg accggctccg agatcgcgtt gcgcgtgcgt ccaacgcagc cgcgtctcga
      601 cgtgccgatc gcgagcttcc gctggaatct tgcgcagaag gaagacgaat acacgctgca
      661 cctcgcgaac ctgcacgcag aactcggcca gccgccgctc gctgacggta cgccggtgtc
      721 tcgcctcctc gccttgcata cgctgaccgg gaattaccgc gcggcgactt tgcagcgcgg
      781 gcagctgttc ggcgtggcgg gggatgtcgt cgatctcggc attctcggcg aattcgcgcg
      841 cgcgctgcca ttgccgtcga aggcgcaaaa cgatctgatc cgcttcaatc cgcgtggcca
      901 gattgccaac tacacgctgc agaccgagcg gcccagaccg gcgacccacg ctcaaatgct
      961 ggctcagcgg cagacgggtg ccgagccgcc aatgcgctac acggtgaagg gcgacttgcg
     1021 tggcgtaagc gttcaggccc aggaaccgcc gccgggattg acgatcaaca accatccgcg
     1081 ggccgggatt ccgggcgtcg agaacctttg gggcagtatt gatgccgatc aggatcatgg
     1141 cagcgcggtc atcgatacaa aaaataccgc gatcaccctg cccggcgtct tcgataaccc
     1201 gcgtctcatg ctcgatcaga tcgacggccg cgtaacctgg accctcgctg aggcgcccgg
     1261 caagccgcac aaggcgatca ctgcgcacgt ggagacgctg cacgtcagga acgcggacgc
     1321 cgaagcgagc gcgacggcgg attaccgaaa cgaaggtgag ggccgcggct ccctcgatct
     1381 caaggcccgg gccgcgcgcc tgaatgtaac gagcctcgtg cactatctgc cgacgagcat
     1441 cagcgaaaag ttgcgcctgt atctgggtca cgcgttgcag gccggtatct cgcgcaatgg
     1501 caccatcgag attcatggtg acctgacgaa attcccctat tcgcgtttcc ccgatgccgg
     1561 ccagttccgc atcgtcgcgc cgttcaccgg cggcaagttc gagccaacgc cctatccgcc
     1621 caggaccatg gcgaacggca cgccgaatgt gtggccggcg tttgacggta tcgacggaac
     1681 gtttcatctc gccgaaaaca agctgggatt cgatatttca cgcggccgtt acctcggcgt
     1741 gagcgtggtc aagacgacgg gacgcattga agacctggga aatcgcgaat cgaaactggt
     1801 gatcgacgcc gacgcgcacg gcccggtcac cgacatgctt cactacgtgg aagacagctc
     1861 gcttggcgcg ctttcacatc atgtgtcgac gaaaatccat gcaaccggta ccgccgcgct
     1921 tgcgctgcaa ctggaaatac cgcgggttgc gccgccgccc ggcgtacggc cgcacgtggg
     1981 ttacaaaggc gcgctcacgt tcaacggcaa tgagctcgcg tacggtgcgt tgccgccgct
     2041 gtcgaacctc aaggggcgga cgcggttcgg cgagaaaacc gcatcgctcg atggcctcac
     2101 ggcgaaattc cttggcggcg atgtgcgcgg caccggtggt gtaaaacccg acggaagtta
     2161 cgccttcaac ataaacggcc gcattggcgg ggacgctgtc caaagcctga atgcccacga
     2221 tcaggcgcag gccgcgcaga ttctcaagca catcagcggc accgcacctt acgcgctgcg
     2281 tgtgcgtggt gtgaaaaacg cgttgccgca agtcgaggcg aactccgatt tgaccggcct
     2341 cggcctggac ttccctgctc cgttcaacaa gagcgagggc acgcccatgc ctgtctcgtt
     2401 cacgttcacg ccggtcgccg ggtcgcctca ggagcacgac gcgacactca cgttcggccc
     2461 ggtgaacgcg cattacctgc tcaagcaaac ggctaaggaa atgccgatcg tggaacgggg
     2521 tgctattggc gtgaacaaac cggccgatct gccggcgcag ggcgtgacgg cggcggtgga
     2581 tatcgacgaa ctcgacgccg atgcgtggcg caagctgatc gccgaattgg gaccgttgcc
     2641 gcaaccggat ccgaagatgc aatcgcaggt ggtgaagcaa ttcatcccca agcgcttcgc
     2701 gctgcatttc gccacgatca agctgctcaa ccggcgctgg gaagatgtgg cgataggcgc
     2761 aaatctcggc gatgaccgca agtggcaggc gaacattgcg tcggatcagg tcacgggcaa
     2821 tgcagaatgg atgccgggcg cgacgaaggg cagtccgggt gcgttgcagg cgcggttcac
     2881 gaaggtggtg attccgggga aggaggaaac cgacctggtt ggacagatga tccgcaagcc
     2941 gccggccaac atgccgtcca tcgatttgat cgtgaacgaa ctcgtggtgc gcgggcgcac
     3001 gctcggacgc cttgaagtgg acgcccacaa tgacgtggaa aacaaggtgc cagtctggta
     3061 tctggacaag ctggacttga gcaacccgga cgcgacgcta tctgctacgg ccaactggcg
     3121 cacgatcagc ggtcccgttg gttttccagc caacgccgac gacaacgccc cccgccgcac
     3181 cgcgctggat ttcaagctgg acatcacgga tgccggcgcg ctggtcgaac ggctgggcct
     3241 gccgcgcacg gtgaaaaatg gcaaaggcac cctgacgggc cagcttggct gggacggcag
     3301 cccggccgcg gttgatttgc ccacgcttaa cggccgactg aatgtcgact tgcaccacgg
     3361 tcagattctc aaggtcaatc ccggcgcggc caagctgctc ggcgtcctca gcctgcagag
     3421 ccttgcacac ttcctcacgc tgaatttccg ggacgtggtc ggcaaagggc tgccgttcga
     3481 aaagatcacc ggctcgggca cgattcagaa tggcatcggc cgcaccaatg atttcacgat
     3541 gatcacggcg ccggcacgcg ttgagctgaa agggctggtc aatttgccga aggaaaccca
     3601 ggacctgcac gcgaaggtca tcccgaccat cagcgcgggc gccgtggcgc tgggcgcggc
     3661 ggtgatcaat cctttgctcg ggctcggcac gctggtggcc gatctggtgc tctcgaagtc
     3721 gattggaaaa gcgttcacaa ttgactattc cattaccggt tcgtggtcga aacccgtcat
     3781 tcagcgggta aaaggcgatc agggtaagat
//
LOCUS       DATASH010001041         8142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4132500,
            whole genome shotgun sequence.
ACCESSION   DATASH010001041 DATASH010000000
VERSION     DATASH010001041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 8142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8142
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4132500"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            255..1436
                     /locus_tag="VHV99_27625"
     CDS             255..1436
                     /locus_tag="VHV99_27625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012733906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HEX3639184.1"
                     /translation="MSNLPGLQFPLGEEIEMLRDSIAGFAAKEIAPRAAEIDRTDQFP
                     MDLWRKFGDLGVLGMTVSEEYGGANMGYTAHMIAMEEISRASASVGLSYGAHSNLCVN
                     QIHRNGTAAQKEKYLPRLVSGEHIGALAMSEPNAGSDVVSMKLRADKKGDHYVLNGTK
                     MWITNGPDCDTLVVYAKTDPEANSRGITAFIVEKGMKGFSVAQKLDKLGMRGSHTGEL
                     VFENVEVPEENILGQLNGGAKVLISGLDYERAVLAGGPTGIMVAVMDAVVPYIHDRKQ
                     FGQSIGEFQLIQGKVADLYTTLQACRAYLYAVGRQLDTLGTQHVRQVRKDCAGVILYT
                     AEKATWMAGEAIQILGGNGYINEYPVGRLWRDAKLYEIGAGTSEIRRMLIGRELFAET
                     A"
     gene            1617..3224
                     /locus_tag="VHV99_27630"
     CDS             1617..3224
                     /locus_tag="VHV99_27630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013690312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX3639185.1"
                     /translation="MPIIESKLNPRSDDFRTNAAALEAVVADLRAKVEKLALGGGQAA
                     RDKHTGRGKLLPRERIEKLLDPGTPFLEFSQLAAYGMYHNDAPGAGVITGIGRIAGQE
                     CVIVCNDATVKGGTYYPVTVKKHVRAQEIAAENHLPCVYLVDSGGANLPNQDDVFPDR
                     DHFGRIFFNQANLSAAGIPQIAVVMGSCTAGGAYVPAMSDESIIVKNQGTIFLGGPPL
                     VKAATGEVVSAEDLGGGDVHTRLSGVVDHLAQNDAHALGIARSIVGNLNRTKQVPVAL
                     QEPKPPRYDVKSMYGVIPVDTRKPFDIREVIARIVDDSAFDEFKARYGTTLVCGFAHI
                     WGHPVGIIANNGILFSESALKGAHFIELCCQRKIPLVFLQNITGFMVGRKYENEGIAR
                     NGAKMVTAVATAKVPKFTVIIGGSFGAGNYGMCGRAYSPRFLWMWPNARISVMGGEQA
                     ASVLATVKRDGIEGKGGSWSAEEEEAFKQPIRDQYEHQGHPYYASARLWDDGVIDPAQ
                     TRDVLGLGLSATMNAPIEDTRFGVFRM"
     gene            3254..4039
                     /locus_tag="VHV99_27635"
     CDS             3254..4039
                     /locus_tag="VHV99_27635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX3639186.1"
                     /translation="MQYETLNVAFAGRIATVTLNRPEVRNAFNETMIAEVTSAFTALN
                     ARDDVRAVVLAANGKAFCAGADLNWMKKMAGYSDDENRADAMLLANMLSSIYRCNKPV
                     IARVNGDAYAGGMGLISACDIVVAVESARFCLSEARLGLIPATIAPYVIRALGEQASR
                     RYFTSAEQFDCATALRLGLVSEAVSAEQLDATVQQIAETLCANGPQAVRACKQLVQDI
                     AGHELNEAMIEDTAARIARTRAGAEGREGVASFLEKRTPSWRD"
     gene            4151..6187
                     /locus_tag="VHV99_27640"
     CDS             4151..6187
                     /locus_tag="VHV99_27640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HEX3639187.1"
                     /translation="MFNKILIANRGEIACRVAATCKRLGIASVAVYSDADANAKHVAA
                     CDEAVHIGGSTAAESYLRVERIIEAARATGAQAVHPGYGFLSENEDFAQACEAAGIVF
                     IGPPVEAIAAMGSKAAAKALMHAAAVPLVPGYHGDDQDPQLLHREADAIGYPVLLKAS
                     AGGGGKGMRVVERTEDFAAALASCKREAASSFGNDRVLIEKYLTRPRHVEVQVFADRH
                     GGAVYLFDRDCSVQRRHQKVLEEAPAPGLSVEIKREMGEAAVAAARAVNYVGAGTVEF
                     IMTATGDFYFMEMNTRLQVEHPVTEMVTGQDLVEWQLRVAADEPLPLTQQQLKIDGHA
                     IEARIYAEHPARGFLPSTGTLKHLRMPEGVEFTIGAAGLGEGSKAPVRIDSGVREGDT
                     ITPFYDPMIAKLIVHGATREEALARLNRALRACEVVGPHTNVEFLQRIVTSEPFATGD
                     LDTGLIERHHDALFAPVKKPFKEALALACAALLTREGGTAHGASPWDALSHWRLNSGY
                     TQTLGWRTIDDNSDNASSFTVTFARDGGTQTLEHDGVRDGFTWSDGTDRHAYRATIGD
                     ARVTGRVFIDGDTFHVFCLGEALAFEWQNLLAHAADAEGGEGRLTAPMPGKVIAVLVE
                     PGTVVEKGTPLIVMEAMKMEHTIGAPAAGTVSEVLYAVGDQVADGAQLLVLDVS"
     gene            complement(6361..7152)
                     /locus_tag="VHV99_27645"
     CDS             complement(6361..7152)
                     /locus_tag="VHV99_27645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006046560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HEX3639188.1"
                     /translation="MTWIAALPMYNVTPALASAWREWLDEVLRMAKPACRIVEPDEEL
                     HGFWRRPNVLLSQTCGYPLMHGLHEQVQVIATPRFDAPGCDGADYSSVLVTRAQASFD
                     SLAACRGGRAAYNQDDSNSGMNVFRHAVAPLARDRAFFSAVLRTGSHLGSLRAVVDDR
                     ADIAAIDCVTFAFVCDELPELARKVRQIGVTAASPGLPLIASNNVPPATIETLREALN
                     EAQLAQPERAKRLRLKGFSALSLIEYERIERLEIEARALGYARLV"
     gene            complement(7171..7449)
                     /locus_tag="VHV99_27650"
     CDS             complement(7171..7449)
                     /locus_tag="VHV99_27650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018423012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 family protein"
                     /protein_id="HEX3639189.1"
                     /translation="MSVYVDEGFRPCVTECATVAFRVCFDDRVTVFEVSAEALMTYCG
                     AASKRRSDLLVAFEGARMEILKVVECKWPFKQSEPVRLGLDEFRMVRH"
     gene            7698..>8142
                     /locus_tag="VHV99_27655"
     CDS             7698..>8142
                     /locus_tag="VHV99_27655"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEX3639190.1"
                     /translation="MKLYYHPLSGHSHRARLLLSLLGVDHELIEVDLMKGAHKSAEFL
                     KLNRFGQVPVLVDDATVVADSNAILVYLAGKLNQTDWLPQTPAAEAAIQRWLSVAAGE
                     IAYGPAAARLITVFSAARNAEEVIGRAHSILGIVEQELGGHEFIVG"
BASE COUNT         1654 a         2492 c         2627 g         1369 t
ORIGIN      
        1 caatcgcctc ggtcacaccg atcccatcac tttatagcgg ctccagacgc atgccaaccg
       61 gtttctgagt gacactcaaa aatacctatt gacgcgtacg taaatctggc gtgaaatgcg
      121 ccttgaacgg ttcgcgcctc gtgcgggacg aggagggcgg cccacagggt ccccgtcgtc
      181 cgctgagccg cactcagacg accggcgccc acccaggctg atccggtccg ccaacgaact
      241 ctggagacac agcaatgagc aacctgcccg gtttgcaatt cccgctcggc gaagaaatcg
      301 aaatgctgcg tgacagcatc gccggcttcg cggcgaaaga aatcgccccg cgtgccgcgg
      361 aaatcgatcg cacggatcag ttccccatgg acctgtggcg caagttcggc gatctgggcg
      421 tgctcggcat gacggtctcg gaggagtacg gcggcgcgaa tatgggctac acggcccaca
      481 tgatcgcgat ggaagaaatc tcgcgcgcct cggcatcggt cggtctctcg tacggcgccc
      541 actcgaatct gtgcgtcaac cagattcatc gcaacggcac ggctgcgcaa aaggaaaaat
      601 atctgcccag gctggtctcg ggtgagcaca tcggcgcact cgccatgagc gagccgaatg
      661 cgggctcgga cgtcgtcagc atgaaactgc gtgcggacaa gaagggcgat cactatgtgc
      721 tgaacggcac gaaaatgtgg atcaccaacg gcccggattg cgacacgctg gtggtctacg
      781 caaaaaccga tcccgaagcg aattcgcgcg gcatcacggc gtttatcgtc gagaagggca
      841 tgaagggctt ctcggtcgcg cagaagctcg acaagcttgg catgcgcggt tcacacaccg
      901 gtgaactggt gttcgaaaac gtggaagtgc cggaagaaaa tattctcggc cagctgaacg
      961 gcggcgccaa ggtgctgatt agcggcctcg attacgaacg cgccgtgctc gccggcggcc
     1021 cgaccggcat catggtcgcg gtcatggacg cagtcgtgcc gtatatccac gaccgcaagc
     1081 agttcggtca gtcgatcggt gaattccagc tgattcaagg caaggtcgcc gatttgtaca
     1141 ccacgctgca agcgtgtcgc gcatatctct acgcagtggg ccggcaactc gatacgctcg
     1201 gcacgcaaca cgtgcgccag gtgcgtaagg actgcgcggg cgtgattctc tacaccgccg
     1261 aaaaagcgac gtggatggcc ggcgaggcga tccagattct cggcggtaac ggctatatca
     1321 acgagtatcc ggtcggccgt ttgtggcgcg atgcgaagct ctatgaaatt ggcgcgggta
     1381 ccagcgaaat ccgccgcatg ctgatcggcc gcgaattgtt cgctgagaca gcctgagcca
     1441 gagtactgca acgcaggcca acaaaacgcg gcgatagcaa ccacgttgca tggaaccaga
     1501 gtgatctgca tgggaccgga gtaagcgcta aagcactaac tccgatcgac aactctgcat
     1561 gggatcggag taagtgctaa agcactgact ccgatcgaca aaggacaagc cacacaatgc
     1621 cgatcatcga atcaaagctg aatccgcgct cggacgactt ccgcaccaat gcggcggcgc
     1681 tcgaagcggt ggtcgccgat cttcgcgcga aggtcgagaa actcgcgcta ggcggcggcc
     1741 aggcggcgcg cgacaaacac acgggccgcg gcaaactgct gccgcgcgag cgcatcgaga
     1801 aactgctcga tccgggcacg ccgtttctcg agttctcgca acttgcggcc tatggcatgt
     1861 accacaacga cgcgccgggc gcaggcgtca ttaccggcat tggccgcatc gcggggcagg
     1921 agtgcgtgat cgtctgcaat gacgcgacgg tcaaaggcgg cacctactat cccgtcaccg
     1981 tgaagaagca cgtccgcgcg caggaaatcg ccgcggaaaa tcatctgccg tgcgtgtacc
     2041 tggtcgactc cggcggcgcc aatctgccga atcaggacga cgtgttcccc gatcgcgatc
     2101 acttcggccg tatcttcttc aaccaggcga atttgtccgc ggcaggcatt ccgcaaatcg
     2161 ccgtcgtgat gggttcgtgc acggccggcg gcgcgtatgt gccggccatg agcgatgaat
     2221 cgatcatcgt caagaaccag gggacgattt ttctcggcgg cccgccgctc gtgaaagccg
     2281 caaccggtga agtggtgagc gcggaagacc tcggcggcgg tgacgtgcat acgcggctct
     2341 ctggcgtggt cgatcatctg gcgcagaacg acgcgcatgc gttggggatc gcgcgcagca
     2401 tcgtcggcaa tctgaatcgc acgaaacagg tgccggtggc gttgcaggag ccgaagccgc
     2461 cgcgctacga cgtgaaaagc atgtacgggg tgattcccgt cgatacgcgt aagccgttcg
     2521 atatccgcga ggtgatcgca cgcatcgtcg acgattcagc tttcgacgaa ttcaaagcgc
     2581 gctacggcac cacactcgtc tgcggcttcg cgcatatctg ggggcatccg gtcggcatca
     2641 tcgcgaacaa cggcattctg ttttcggagt cggcgctcaa gggcgcgcac ttcatcgaac
     2701 tgtgttgcca gcgcaagatt ccgctcgtgt tcctgcagaa catcaccggt ttcatggtcg
     2761 gccgcaaata cgaaaacgaa ggcatcgcgc gtaacggcgc gaagatggtg acggctgtcg
     2821 ccacggcgaa ggtgccgaag ttcacggtga tcatcggcgg ctcgttcggc gcgggcaatt
     2881 acggcatgtg tggtcgcgcg tattcaccgc gcttcctgtg gatgtggccg aatgcacgca
     2941 tttcggtgat gggcggcgaa caggcggcct cggtgctggc cacggtcaag cgcgacggca
     3001 tcgaaggcaa gggcggttcg tggagcgccg aagaggaaga agcgttcaag caaccgatcc
     3061 gcgaccaata cgagcatcag ggccacccgt actacgcgag cgcgcgcttg tgggacgacg
     3121 gcgtgatcga tccggcgcaa acgcgcgacg tgctcggtct cggcctctcg gcgacgatga
     3181 acgcgccgat cgaagacacg cgtttcggcg tgttcagaat gtgacggagc gctacgcgag
     3241 aggagctgca acgatgcaat acgaaacgct gaatgtcgct tttgctggtc gcatcgccac
     3301 ggtcacgctg aatcggccgg aggtgcgcaa cgcgttcaac gaaacgatga tcgccgaagt
     3361 gacctcggcg ttcacggccc tgaacgcgcg cgacgacgtg cgcgcggtgg tgctcgccgc
     3421 gaacggcaag gcgttctgcg cgggcgccga tctgaactgg atgaagaaga tggccggcta
     3481 ctcggacgac gagaatcgtg ccgacgcgat gctgctggcg aacatgcttt cgtcgatcta
     3541 tcgctgcaac aagccggtga tcgcgcgcgt gaatggcgac gcatacgcgg gcggcatggg
     3601 cttgatctcg gcgtgcgata tcgtcgtcgc ggtggagagc gcgcgttttt gcctctcgga
     3661 agcgcgtctc ggtctgatcc cggccaccat cgcgccctat gtgatccgcg cgttgggcga
     3721 gcaggcgtcg cgccgctact tcacgagcgc cgagcagttc gattgcgcga cggcgttgcg
     3781 cctgggcctc gtcagtgaag cggtgagcgc ggagcaactc gacgccaccg tgcagcagat
     3841 cgctgaaacg ctgtgcgcga acggcccgca agcggtgcgt gcctgcaagc aactcgtgca
     3901 ggacatcgcc ggccacgaac tgaacgaggc catgatcgag gacacggcgg cgcgcatcgc
     3961 gcgcacgcgc gcgggtgcgg aaggccgtga aggcgtcgca tcgttcctcg aaaaacgcac
     4021 accgtcctgg cgcgattgaa gcgtcaattg aagcgtcaat tgacgcgcca cgccggcatc
     4081 cattccaggg cgcatgcgcg ccttgaccag aacaacacag ccccgagcgg gacaagcacc
     4141 gagacacctc atgttcaaca agatcctgat tgcgaaccgc ggcgagattg cgtgccgcgt
     4201 cgccgcgacc tgcaagcggc tcggtatcgc gagcgtggcc gtctattccg acgcggatgc
     4261 caacgcgaaa cacgtggccg cgtgtgacga ggccgtgcat atcggcggct cgaccgcggc
     4321 agaaagctat ctgcgtgtcg agcgcattat cgaagccgcg cgcgccaccg gtgcgcaagc
     4381 agtgcatccg ggctacggct tcctgtcgga aaacgaagac ttcgcgcagg cctgcgaagc
     4441 ggccggcatc gtcttcatcg gaccgccggt cgaggcgatc gccgcaatgg gctcgaaggc
     4501 ggccgcgaag gcgctgatgc atgcggccgc ggtgccgctc gtgccgggtt atcacggcga
     4561 cgatcaggat ccgcagctgc tgcatcgcga agcggatgcg atcggctatc cagtcttgct
     4621 gaaggcgagc gcgggcggcg gcggcaaagg catgcgcgtg gtcgagcgca cggaagactt
     4681 cgcggcggcc ctggcgtcgt gcaaacgcga agcggccagc agcttcggca acgaccgcgt
     4741 gctgatcgaa aagtatctga cgcggccgcg tcacgtggaa gtgcaggtgt tcgcggaccg
     4801 tcacggcggc gcggtgtatc tgttcgaccg cgactgctcg gtgcagcggc gtcaccagaa
     4861 agtgctggaa gaagcgccgg cgccaggttt gtccgtggag atcaagcgcg agatgggcga
     4921 agcggccgtg gccgccgcgc gtgcggtgaa ttacgttggg gcgggcacgg tcgagttcat
     4981 catgaccgcc acgggcgatt tctacttcat ggagatgaac acgcgcctgc aggtcgagca
     5041 tccggtcacg gaaatggtga ccgggcaaga cctggtggaa tggcaactgc gcgtcgccgc
     5101 ggacgaaccg ctgccgctca cgcagcaaca gctgaaaatc gacggccacg cgattgaagc
     5161 gcgcatttac gccgagcatc cggcacgcgg cttcctgccg tccactggga cgttgaagca
     5221 tctgcgcatg ccggaaggcg tcgaattcac catcggcgcg gccggcctgg gcgagggcag
     5281 caaagcgccg gtacgcatcg acagcggcgt gcgcgaaggc gataccatca cgcccttcta
     5341 cgatccgatg atcgccaagc tgatcgtcca cggcgcgacg cgtgaagagg cgctggcgcg
     5401 tctgaatcgc gcgctgcgtg cctgcgaagt ggtggggccg cataccaacg tcgagttcct
     5461 gcaacgcatt gtcaccagtg agccgttcgc gaccggcgat ctcgatacgg gtttgatcga
     5521 gcgtcatcac gacgccctgt tcgcgcctgt gaagaagccc ttcaaggaag cgctggcgct
     5581 cgcctgcgcc gcgttgctga cgcgcgaagg cggcactgcg cacggcgcct cgccgtggga
     5641 cgcgctatcg cactggcgtc tgaacagcgg ctatacgcag acgctcggct ggcgcactat
     5701 cgacgacaac agcgacaacg caagctcctt caccgtcacg ttcgcccgtg acggcggcac
     5761 gcaaacgctc gagcatgacg gcgtgcgtga tggcttcacc tggtcggacg gcacggaccg
     5821 gcacgcatac cgtgcgacga tcggcgatgc gcgggtaaca ggccgcgttt tcatcgacgg
     5881 cgatacgttc cacgtgttct gtctcggcga ggcgctggcg ttcgagtggc aaaacctgct
     5941 cgcgcatgcc gcggatgccg aaggcggcga aggccgtttg accgcgccga tgccgggcaa
     6001 ggtgatcgcg gtgctggtcg aaccgggcac ggtagtggag aagggcacgc cgctgatcgt
     6061 gatggaagcg atgaagatgg agcacacgat cggcgcaccg gcggcaggca cggtgtcgga
     6121 agtgttgtat gcggtcgggg atcaggtcgc cgatggggcg cagctgctcg tgctggatgt
     6181 gagctaagcg cgaggtttaa acgaaccgta agcgaggccc aaacgaaacc tgaacgaggc
     6241 ggaacggggg ctaagcgacg cctaagcgag actcaaacga ggcctaagca agacctaagc
     6301 aagacctaag caatacctaa gcaataccta agcaatgcct aagcaaggcc taagcaagac
     6361 tcaaacgagg cgcgcatagc ctaacgcccg cgcttcgatt tcgagtcgct cgatccgttc
     6421 gtactcaatg agcgacaacg cggaaaagcc cttcaaacgc aggcgtttcg cgcgttcggg
     6481 ctgtgccagc tgcgcttcgt tcagtgcctc gcgcaacgtt tcgatggtcg cgggcggcac
     6541 gttattggaa gcgatcaaag gcaaacccgg cgacgccgcc gtcacgccga tctgccgaac
     6601 ctttcgcgcg agctccggca attcatcgca gacaaacgcg aacgtcacac agtcgatggc
     6661 ggcgatgtcc gcgcgatcgt cgaccaccgc ccgcaacgat ccaagatgcg aaccggtgcg
     6721 caggaccgcg ctgaaaaacg cgcggtcgcg cgcaagcggc gccaccgcgt ggcgaaacac
     6781 attcatgccg ctgttcgaat cgtcctgatt gtaggcggca cggccgccac ggcacgccgc
     6841 aagcgagtcg aacgacgcct gggcccttgt gaccagcaca ctcgaataat ccgcgccgtc
     6901 gcagccgggc gcatcgaagc gcggcgtggc gatcacctgc acctgctcgt gtaaaccatg
     6961 catcaaggga tagccgcagg tttgcgagag caggacgtta gggcgccgcc agaagccatg
     7021 caattcctcg tcgggctcga cgatgcggca cgccggcttc gccatgcgca agacctcgtc
     7081 cagccactcg cgccacgcgc tggcgagcgc gggcgtcacg ttgtacatcg gcagggcggc
     7141 gatccaggtc atgacgcgat tctgtcatac ctaatgtctg accattcgga attcgtcgag
     7201 ccccaaccgc acgggctcgc tctgcttgaa cggccatttg cattccacca ccttgaggat
     7261 ctccatccgc gcgccttcga acgcaacgag gaggtcgctg cgccgtttgc tcgcggcgcc
     7321 gcagtaggtc atcagtgcct cggcgctgac ctcgaagacc gttacccgat cgtcaaaaca
     7381 gacccggaac gcgacggtcg cgcattccgt gacacagggc ctgaatcctt catcgacata
     7441 taccgacata gccatctcct gccgcctacc tgagtcatca ggatgagcgg ccgtaagctt
     7501 tcagtatgtt ggaaaaccgg cacttggcgc aagaaaccgc ggcgccccgg ctgggacgga
     7561 tcagggcgcc agcactgttc tcgttacgag aatagtgatt tgacctggcg acgtattctg
     7621 gaaagcaggt ggcctcccta gacttcctcc cagtggttcg gatttcccag ccacaccgcc
     7681 aaccaaggaa gttcgccatg aagctctatt accacccgct gtccggtcac tcgcatcgcg
     7741 cccgcctgtt gctgtcgctg ctcggcgtcg accatgaact catcgaggtc gatctgatga
     7801 agggcgcgca caagtcagcg gaattcctca agctgaaccg tttcggccag gttccagtgc
     7861 tggtggacga cgctaccgtg gtggcggatt cgaatgcgat tctcgtgtac ctcgccggca
     7921 agctgaacca gacggactgg ctgccgcaaa cgccggccgc ggaagcggcg atccagcgct
     7981 ggctttcagt ggccgccggg gaaatcgctt acgggccggc ggcggcacgc ctgatcacgg
     8041 tgttcagcgc ggcgcgcaat gcggaggaag tgatcggccg tgcgcatagc attctcggca
     8101 tcgtcgagca ggaacttggc gggcacgagt ttattgtcgg ca
//
LOCUS       DATASH010001042         6047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3345630,
            whole genome shotgun sequence.
ACCESSION   DATASH010001042 DATASH010000000
VERSION     DATASH010001042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6047
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3345630"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VHV99_27660"
     CDS             <1..301
                     /locus_tag="VHV99_27660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR00374 family protein"
                     /protein_id="HEX3639191.1"
                     /translation="LALRFLGHPVDWVDALLLESLGQAIRGAAFMIPGSLGVQEGGYL
                     LLAPLVGLPPDAALALSLAKRAREILLGLPGLLVLHFSERSWQRRRATARVPVVD"
     gene            329..1093
                     /locus_tag="VHV99_27665"
     CDS             329..1093
                     /locus_tag="VHV99_27665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018423586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HEX3639192.1"
                     /translation="MRAIILAAGLGLRLQQPPQAQFPKCLLQFDGMSLLERHLQMLET
                     AGVTEVVLALGFQPESVQAELDRINWPHQVETVLNPRYDLGSVLTVHTVAEALTRGGD
                     VLLMDADVLYDERILSALVAGETVNRLLIDRDFEAGDEPVKLCLKDGVPVELRKQLAV
                     NLEYDTIGESVGFFRFRQQTAQRFTEIVAGYVDSGRANLPHEEAVRDLLLERSQVFDT
                     ADVTGSPWIEIDFPNDVARASTEILPQLQPLVSASR"
     gene            1350..2753
                     /locus_tag="VHV99_27670"
     CDS             1350..2753
                     /locus_tag="VHV99_27670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3639193.1"
                     /translation="MPLALGTFIVPLIVACAMFMENMDGTVIVTSLPVLARDLGQDPI
                     TLKLAVTAYVIGLGVFIPICGWVADRFGSRTVFRTAIGIFMAGSLMCAASTSLGTFVV
                     ARFVQGVGGAMMVPVGRIIIFRSVPKSDFIRAVNYLTVPALLGPVVGPPLGGFITTYL
                     HWRLIFFINIPIGLLGIWLANKHIANVREAHPGRLDWTGFGLSASGASLFMLGLSLVG
                     GELVSNTVALSMCVIGVVLLATYVLYANRVELPVLDLRLLRIPSFHASVVGGSLFRIG
                     LGAVPFLLPLVLQEGLGMTAFKSGSITCASAFGSVFMKAAASRILGRFGFRTVLMFNA
                     GCAGLAIAVYGLFFPGTPHWLIWCVVLFGGFFPSLQFTSLNTLAYADIPSRDVGRATS
                     VASVIQQMSLGLGVTIAGIVLQISHNLQGHSTIVFSDFWPAFLVVGLFSFLSIPVTAR
                     LPHGAGDEIARGARGNA"
     gene            2877..4610
                     /gene="ggt"
                     /locus_tag="VHV99_27675"
     CDS             2877..4610
                     /gene="ggt"
                     /locus_tag="VHV99_27675"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX3639194.1"
                     /translation="MKLFHNARSSASGLALVALVTVSTGLLEATPAFAKAPAKAQPAV
                     LTASAIAVADKYSADAAEQIFKEGGNAVDAAVAIAFTLAVTYPEAGNIGGGGFMTLYV
                     DGKPYFLDYRERAPLAATKNMYLDDKGEVIKGMSLFGYRAVGVPGTVDGMWQAQRRFG
                     KLKWKQVLAPAIHYARDGFEVSEQLQQRRDDAAKDFAGKTNFDTYFGNLKQGVNFKQA
                     DLAAVLQRISDQGAKDFYSGKTADLIAASMRGHGLITKADLQQYKAVWRQPIQADWNG
                     YRVITAPPPSSGGIGLVQLLKIKADIAPDFKDVKLNSAQYVHLIAEIEKRVFADRAQY
                     LGDPDFYKVPVAQLTDDAYIARRAAEINPNSPSDTKSIQAGLGTSMPEKAETTHFSVI
                     DKWGNAVSNTYTINGYFGSGVVADRTGIVLNDEMDDFSAKPGVANMFGVVGSDANSIE
                     PKKRPLSSMSHTILTRDGKVSLVIGTPGGSRIFTSIFQVINNVYDFNMPLPEAVAAMR
                     FHHQLLPPNTIFWEPYKPIDGELAKQIEAKGYSLKGQDFSGDIQAIKVNGDTPEAAAD
                     PRGRGVTRVIQ"
     gene            complement(4691..5737)
                     /locus_tag="VHV99_27680"
     CDS             complement(4691..5737)
                     /locus_tag="VHV99_27680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013342543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX3639195.1"
                     /translation="MGSRPRFIDDLSRAASDAPGSDPLSALSDDALLDAYSRTVIGAL
                     ERVQQAVAFISVERRLPGAPSGRDSRGARGGTGSGFLFTPDGYLLTNSHVVHGATHIT
                     VTLADGAKFAADLVGDDPGSDLAVLRIGSPEPLPHVELGESSKLRVGQIAIAVGNPLG
                     LAQTVTTGVVSALGRSLRSNSGRMIYDVIQTDAALNPGNSGGPLINSAGQVIGVNTAI
                     IPGAQAICFATAIDTAKWVIMQIFAHGRVRRAYIGVAGTTRPLSRRVQRYFGWSVESG
                     VHVMEIVKGSPAALGGLRTGDTIVAIDTLAVQDVDSLQRTLDASRIDKPVNVTVLRGA
                     QRLELTLTPVEQTS"
BASE COUNT         1065 a         1837 c         1960 g         1185 t
ORIGIN      
        1 gctcgcgttg cgctttctcg gccatccggt cgactgggtc gatgcgctcc tgctcgaaag
       61 tctcggacag gcaattcgtg gtgcagcgtt catgatcccc ggttcgctgg gcgtgcagga
      121 aggcggttat ctgctgctcg caccgctcgt gggcttaccg ccggatgcgg cgctggcgtt
      181 gtcgctcgcc aagcgcgcgc gggagatcct gctgggtttg ccgggtctac tggttttgca
      241 cttcagcgaa cgaagctggc aacggcggcg cgccacggcg cgcgtgccgg ttgtcgatta
      301 aaactccgta ttttttcaag gactgcgcat gcgtgccatc atcctagcag cgggcctcgg
      361 cctgcgtctc cagcaaccgc cgcaggcaca gttcccgaag tgcctgttgc agttcgacgg
      421 catgagcctg ctcgaacggc atctgcaaat gcttgaaacc gccggtgtga cggaggtcgt
      481 gctggcgctc ggttttcagc cggaatcggt gcaggcggaa ctggatcgga tcaactggcc
      541 gcatcaggtg gaaaccgtgc tgaacccgcg ttacgacctg gggagcgtgc tgacggtgca
      601 cacggtggcc gaggcgctga cgcgcggcgg cgacgtgctg ctgatggacg ccgacgtgct
      661 gtacgacgag cgcattctga gcgcgctggt agcgggcgaa acggtcaacc gcctgttgat
      721 cgaccgcgat ttcgaagccg gcgacgaacc cgtcaagctg tgcctgaaag acggcgtgcc
      781 ggtcgaattg cgcaagcagt tagccgtcaa tcttgagtac gacaccatcg gcgaatcggt
      841 gggcttcttc cgcttccgcc agcaaaccgc gcagcgcttc acggagattg tcgcgggcta
      901 tgtggatagc ggccgggcca acctgccgca tgaagaagcg gtgcgtgacc tcctgctgga
      961 gcgcagccag gtgttcgata cggccgacgt gaccggctcg ccgtggatcg agatcgattt
     1021 cccgaacgac gtggcgcgcg caagcaccga gattctgccg cagctgcaac cgctggtcag
     1081 cgcgtcgcgt taaatcccgc tcctgaatgg gtactgtatt gcatggtgag ccggttgtac
     1141 gccggccacc atcgcgtcgt tttcccgctt cttttcccct cctgtttttc gtagtcagtg
     1201 ctgcctcgtt gcgcgcacaa cgattggacg cgggatagcg gtgcgtcatg cgtgtgcgca
     1261 aaaaggcatt gcccttgcaa catctgttgt cgatgcaatg cgataattgc cgctttacac
     1321 cggcggtctt gcaacccgtc gccatgccaa tgcctcttgc tttaggcact ttcatcgttc
     1381 cgctgatcgt cgcgtgtgcg atgttcatgg aaaacatgga cggcacggtc atcgtgacgt
     1441 cactgccggt tctggcgcgc gatctcggcc aggatcccat caccctcaag ctcgccgtga
     1501 cggcatacgt catcggcctc ggcgtcttca ttcccatttg cggctgggtc gccgaccgct
     1561 tcggttcgcg cacggtgttt cgcacggcaa tcggcatctt catggccggt tcgctgatgt
     1621 gcgcggcgtc cacgtcgctg ggcaccttcg tggttgcgcg tttcgtgcaa ggcgtcggcg
     1681 gcgcgatgat ggtgccggtg gggcgcatca tcattttccg ctcggtgccc aagtcggact
     1741 tcatccgcgc ggtcaactat ctgacggtgc ccgcgctgct cgggccggtg gtcggcccac
     1801 cgctcggcgg cttcatcacg acatatctgc actggcggct gatcttcttc atcaatattc
     1861 caatcggtct gctgggtatc tggcttgcaa acaagcacat cgccaacgtg cgcgaagcgc
     1921 atcccgggcg gctcgactgg accggtttcg ggttgtcggc gagcggggct tcgctattca
     1981 tgctgggact ctcgctggta ggcggcgaac tcgtgtccaa cacggtagcg ctcagcatgt
     2041 gcgtgatcgg cgtggtgctg ctggcgacgt acgtgctgta tgcgaaccgc gtcgagttgc
     2101 cggtgctcga tttgcggctg ttgcgcattc cgagttttca cgcgagcgtg gtgggcggct
     2161 cgctgtttcg catcggcctc ggcgcggtgc cgtttctatt gccgcttgtg ttgcaggaag
     2221 gcttgggcat gaccgccttc aagtcgggat cgatcacctg cgcatccgcg ttcggttcgg
     2281 tcttcatgaa ggcggcggcc tcgcgtattc tcggccgttt cgggttccgc acggtgctga
     2341 tgttcaatgc cggctgcgcg ggtctcgcca tcgcggtcta cgggttgttc tttcccggta
     2401 cgccgcattg gttgatctgg tgtgtggtgc tgttcggcgg cttcttcccg tcgttgcagt
     2461 tcacctcgtt gaataccctg gcgtatgcgg acattccgag ccgcgacgtg ggacgggcga
     2521 cgagtgtcgc cagcgtgatc cagcaaatgt cgctggggct cggcgtgacg atcgcgggca
     2581 tcgtgctgca gatttcgcac aacctgcagg gtcattccac gatcgtcttc tccgacttct
     2641 ggccggcgtt tctggtagtc ggcctgttct cgttcctgtc gattccggtg accgcgcgct
     2701 tgccgcacgg ggcaggcgat gaaatcgcac gcggcgcccg cggcaacgcc tgactttgtg
     2761 tgccgcagca tgcaccatat gcgtgcaatt gtttcgcaaa tggcggagcg gcatgttata
     2821 agcccaaggc agctcttgtt atcgtcagat caagaagtcg tcttggggga cattcaatga
     2881 agttgtttca caatgccaga tcgtctgcta gcggacttgc gctagtcgca ctcgtcaccg
     2941 tttcaaccgg cttgctcgaa gccactccgg ccttcgccaa agcgcccgcg aaagctcagc
     3001 cggcggttct tacggcctcc gcgatcgcgg tggccgacaa gtacagcgcc gacgccgcgg
     3061 aacagatctt caaggaaggc ggcaatgccg tcgacgcagc cgttgcgatt gccttcacgc
     3121 ttgccgtgac ctatcccgaa gcgggcaaca ttggcggcgg cggcttcatg acgctctatg
     3181 tcgacggcaa gccgtacttc ctcgactatc gcgagcgcgc tccgctggcc gcgaccaaga
     3241 acatgtatct cgacgacaag ggcgaggtca tcaagggcat gagcctgttc ggctatcgcg
     3301 cggtaggcgt gccgggcacc gtcgacggca tgtggcaggc ccagcgccgc ttcggcaagc
     3361 tcaagtggaa gcaggtgctc gcaccggcca ttcactacgc acgcgacggc ttcgaggtca
     3421 gcgagcaatt gcagcaacgc cgcgacgacg ccgcgaaaga ttttgccggc aagaccaact
     3481 tcgataccta cttcggcaac ctgaagcagg gcgtcaactt caagcaagcg gatctcgccg
     3541 ccgtgctgca gcgcatttcg gatcagggcg cgaaagactt ctactcgggc aagacggccg
     3601 atctgatcgc ggcctccatg cgcggccacg gcctgattac gaaggccgac ctgcagcaat
     3661 acaaggcggt gtggcgtcaa ccgatccagg cggactggaa cggctatcgc gtgatcaccg
     3721 cgccgccgcc gagttcgggc ggtattggtc tcgtgcagtt gctgaagatt aaggccgata
     3781 ttgcgcccga cttcaaggac gtcaagctga attcagcgca atacgtgcac ctgatcgcgg
     3841 aaatcgagaa gcgtgtgttc gccgatcgtg cgcagtatct cggcgatccg gatttctaca
     3901 aggtgccggt cgcgcaattg accgacgacg cgtatatcgc caggcgtgcc gcggaaatca
     3961 atccgaactc accttcggac acgaagagta ttcaggccgg gctcggcacg tcgatgcctg
     4021 agaaagcgga aaccacgcac ttctccgtaa tcgacaagtg gggcaatgcc gtatcgaata
     4081 cgtacaccat caacggttat ttcgggtcgg gcgtggtggc ggatcgcacg ggcatcgtgc
     4141 tgaatgacga aatggacgac ttctccgcga agccgggcgt ggcgaacatg ttcggcgtgg
     4201 tgggcagcga cgcgaattcg atcgaaccga agaagcgccc gttgtcctcg atgagccaca
     4261 cgattctgac cagggacggc aaggtgtcgc tcgtgatcgg cacgccgggc ggctcgcgca
     4321 tcttcacgtc gatcttccag gtgatcaaca acgtctacga cttcaatatg ccgttgccgg
     4381 aggctgtggc cgcgatgcgt ttccatcacc aactgttgcc gccgaacacg atcttctggg
     4441 agccgtacaa gccgatcgac ggcgaactcg ccaagcagat cgaagccaag ggctactcgc
     4501 tgaaggggca ggatttcagc ggcgacattc aggcgatcaa ggtcaatggc gatacgccgg
     4561 aagctgccgc cgatccgcgt ggccgtggtg tcacgcgagt gatccagtga gcgtttgaac
     4621 tcactcgttc tggcgcatct gagcggcgca tctttgcggg tgcgccgcac gcaggaaaaa
     4681 ggcctcgcgt tcagcttgtc tgctccaccg gcgtcagggt caattcgagc cgttgcgcgc
     4741 cacgcaacac cgtcacgttg accggcttgt cgatccgcga cgcgtcgagc gtgcgttgca
     4801 ggctgtcgac gtcttgcacc gcaagcgtat cgatcgcgac gatcgtgtcg ccggtacgca
     4861 gaccgccgag tgcggcggga ctgcccttga cgatctccat gacgtgcacg ccactctcca
     4921 cgctccaccc gaaatagcgc tgtacgcggc gcgacaaggg tctcgtggtg ccggccacgc
     4981 cgatgtacgc gcgccgcacg cggccgtgcg cgaagatctg catgatgacc cacttggccg
     5041 tatcgatcgc ggtggcgaag cagatcgcct gcgccccggg aatgatcgcg gtgttcacgc
     5101 cgatcacctg accggccgaa ttgatcagcg gaccgcccga attgccggga ttgagcgcgg
     5161 catccgtctg gatcacgtcg tagatcatgc gacccgaatt cgagcgcagc gagcggccaa
     5221 gcgccgagac cacgccagtc gtcacggttt gcgcgaggcc gagcggattg cctaccgcga
     5281 tcgcaatctg gccgacccgc agctttgacg attcgccgag ttcgacgtgc ggcaatggct
     5341 cgggcgagcc gatgcgcagg acggccagat cgctgccggg gtcgtcgccg accagatcgg
     5401 cggcgaattt cgcgccgtcg gcgagcgtca ccgtgatatg cgtggcgccg tgcacgacgt
     5461 ggctgttggt gagcaaataa ccgtcgggcg tgaacagaaa gcccgagccg gtgccgccgc
     5521 gcgcgccgcg gctgtcgcgc ccggatggcg cgccaggcag ccggcgttcg acggaaatga
     5581 aggcgaccgc ctgctgcacg cgttccagcg cgccgattac ggtgcgggaa taggcgtcga
     5641 gcaacgcgtc gtcggataaa gcgctgagtg ggtcggaccc gggggcgtcg gatgcggcgc
     5701 gcgacaggtc atcgatgaag cgtggacggc ttcccatggc gatgctccgg atgaacggaa
     5761 atcgagatgc ggggcggcgg gcaggttttc aagtgcgggc cggctgtggg gcgcgggggc
     5821 tgggtgtgag acactttctg atgtcggcac gcgcgaacgc tggatcggtg gcgaggctgg
     5881 aacgccggct gatggctcgt ccgacgccgg ggacggagcg ggagccggca gaaccagcag
     5941 aaccagcaga accagcagaa ccagcagaac cagcagaacc agcagaacca gcagaaccag
     6001 cagaaccagc agaaccagca gaaccagcag aacccgggca cacgtca
//
LOCUS       DATASH010001043         2467 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3540603,
            whole genome shotgun sequence.
ACCESSION   DATASH010001043 DATASH010000000
VERSION     DATASH010001043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3540603"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..116)
                     /locus_tag="VHV99_27685"
     CDS             complement(<1..116)
                     /locus_tag="VHV99_27685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007732318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX3639196.1"
                     /translation="MSKQPRLLLLSDLMLLAVAVVWGTSYGVVKSALVFYPVL"
     gene            257..1177
                     /locus_tag="VHV99_27690"
     CDS             257..1177
                     /locus_tag="VHV99_27690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3639197.1"
                     /translation="MNPTDLFALLPDMAVFARVVDAGNFSVAARQLGSTPSTVSRQIK
                     RLEDALATRLLERSTRTVRVTESGAQVVRFCRDMVSAASGAVDAAGQLAGRPQGKVSV
                     SAPTAFAKTVIHPLIAGFLRAYDEVDLQLLFTDHDVDPLADDVDLVIRLTEHPPQGLA
                     GRRLGAVRWLLVASPAYVRERGMPAQPRDLLKHDCIYLGETADDNRWRFRRGTETQSV
                     DVKGRYIANHAGARLEAAQQDFGVASLPEFAAADALLSGELVQVLPDWNLETRAYVGA
                     VWLLYPPNRFLPPKVRALIDYLVEHIHEAA"
     gene            complement(1283..2062)
                     /locus_tag="VHV99_27695"
     CDS             complement(1283..2062)
                     /locus_tag="VHV99_27695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011660988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639198.1"
                     /translation="MQQPDPLHPLRPILKNYVWEHGHIGTRYLNCANGEINFDDGKKS
                     RFAAEKYLYVPLDKDATDDPRVTGPSTQDAALARFLRAAQLGKPEEAGSAAEVQRAVH
                     DCVDLAIFSAYQHDAQQAVDHYTQEAMFDDEIRAALVSDIRRVYGPMREQLALYDFTV
                     LHGLPTPLLFSDSPFIDWRVRVKPAMPFVSLPLGPYALLVGTPSGRTSRAAPVAWKTA
                     VSMGVLKDHNRLMAERARLWIVATSDDQLVAIQDRFKKDDA"
BASE COUNT          408 a          690 c          831 g          538 t
ORIGIN      
        1 agcaccggat agaacacgag ggcacttttg acgacgccgt aactggtgcc ccacaccacg
       61 gcgaccgcga gcagcatcag atcggaaagc agaagcaggc gtggttgttt cgacatggcg
      121 gggcggacct caagcagatg agcacgtatt gcgcacatat tgtcgaactt tgcgccgaag
      181 gcgataatcg ggtcgccatg cacagctttt atgtccaaaa cgcattaata gacgcacctt
      241 cagctggctt ctcacgatga acccaaccga ccttttcgcc ttgctgccgg atatggcggt
      301 tttcgcgcgc gtcgtcgatg ccggcaattt ttcggtggcc gcgcgtcagc tcggcagtac
      361 gccgtctacc gtcagccgtc agatcaagcg gctcgaggac gctttggcga cacgcctgct
      421 cgaacggtcg acgcgcacgg tgcgggtcac ggaatcgggc gcccaggtcg tgcgtttctg
      481 ccgcgacatg gtgagcgccg cgtcgggcgc ggtggatgcc gccgggcaac tggccggcag
      541 accgcaaggc aaggtcagcg tgagcgcgcc gaccgcgttt gcgaagaccg tgattcatcc
      601 gctgattgcc ggcttcctgc gcgcttacga cgaagtcgac ctgcaactgc tgttcaccga
      661 tcacgacgtc gacccgctgg ccgacgatgt cgatctggtg atccgtctca ccgagcaccc
      721 tccgcagggg ctcgcgggac gcaggctggg cgcggtgcgc tggctactgg tcgcgtcgcc
      781 ggcctatgta cgcgagcgcg ggatgcccgc gcaaccgcgc gatctcctca agcacgattg
      841 catttatctg ggcgaaaccg ccgacgacaa ccgctggcgc tttcgccgcg gcacggagac
      901 gcaaagcgtc gacgtgaaag gacgttacat cgccaatcat gctggcgcgc ggctcgaagc
      961 ggcgcagcag gatttcggcg ttgcgagctt gcccgaattt gccgctgccg atgcattgct
     1021 gagcggcgag ctggtgcagg tgctgcctga ctggaacctc gaaacgcgcg cttacgtggg
     1081 ggcggtgtgg ttgctgtatc cgccgaaccg tttcttgccg cccaaggtaa gggcattgat
     1141 cgactatctt gtcgagcaca tccacgaagc ggcttaagca gcgcgggtca agttcaccgc
     1201 gcgacgcgga cgcgcgccat gctcgctcgc gctcgaagcg cattcacacg gccgggcgtg
     1261 aagatacgca gggcggcgcc ggttatgcgt cgtctttctt gaagcgatct tgtatggcga
     1321 ccagttgatc gtcgctggtg gcgacgatcc atagacgggc gcgctcggcc atcagccgat
     1381 tgtgatcctt caggacgccc atcgacacgg ccgttttcca cgcgacgggc gcggcacggc
     1441 tggttcttcc tgacggcgtg ccgaccagca atgcgtaggg tccgaggggt agcgagacga
     1501 atggcatggc cggcttgacg cgcacgcgcc agtcgatgaa gggtgaatcg ctgaacagca
     1561 gcggcgtggg cagaccgtgc agcacggtga agtcgtagag cgcgagttgt tcacgcatgg
     1621 gtccgtagac ccggcgaata tcgctgacga gcgccgcgcg gatttcgtcg tcgaacatcg
     1681 cttcctgggt gtagtggtcg acggcttgtt gcgcgtcgtg ctgataggcg ctgaagatcg
     1741 cgaggtcgac gcaatcgtgg acggcgcgtt ggacttccgc ggccgagccg gcttcttcgg
     1801 gtttgccgag ttgcgcggcg cgcaggaagc gcgcgagggc ggcgtcctgg gttgacgggc
     1861 cggtgactcg tgggtcgtcg gtggcgtctt tgtctaatgg gacgtagagg tatttttctg
     1921 ccgcgaagcg ggattttttg ccgtcatcga agttgatttc gccgttggcg cagtttaggt
     1981 atcgggtgcc gatgtggccg tgttcccaga cgtagttttt caggatgggg cgcaggggat
     2041 ggagtgggtc tggttgttgc attttgcctt ttgcactccg tgcgggttgg gttggggtat
     2101 ggtcacatag aagttggggt tttgttggtt tttttgcctg tgcggcgggt tgtctggttg
     2161 cctgcggcgt tggcccctcc ttgatttgtt tgtttgctcg cgcgtggttg tctgtttgcc
     2221 ttcggcgttg gcctttcctt gatctgggtt tttggccttt ccttgaattg ttattggtct
     2281 attagcgtcg cccctgtgcg gggcaggcac ctacttttct ttgcctgccg cgtaactatt
     2341 cagcggcaga caaaggcgaa acgacgattc cggcaaagct ggaggccagt gcatcaatca
     2401 gcgaatggtt ctgcttacgc aaggtggcaa ggtaggagcg gatcgtacag aacgcttgca
     2461 tgcccga
//
LOCUS       DATASH010001044         1847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2750914,
            whole genome shotgun sequence.
ACCESSION   DATASH010001044 DATASH010000000
VERSION     DATASH010001044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2750914"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            233..1324
                     /locus_tag="VHV99_27700"
     CDS             233..1324
                     /locus_tag="VHV99_27700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03118 family protein"
                     /protein_id="HEX3639199.1"
                     /translation="MHRFATVFSLAGAATLALAACGGNSNNNMPTATAGTFKSTILVS
                     DGSVAAPHVDPNLQNSWGIAFGPTTPVWVSDNNTQKSSLYDGNGVVQSLVVTLPPAAS
                     GQPASPTGIVFNGTTDFAVTANGATGNAAFIFATLAGTIAAWSPKVLPTQAVTVHDDG
                     AGAAEYTGLAIASNAGTSTLYAADFHNGKVDMFDKTFTKVSVAGAFSDPNVPAGFNPF
                     GIQAVGSTIYVAYAKLGPDGHRDQSGAGNGVVDAFDTAGHLIRRIASNGPLNSPWGIA
                     MAPANFGAFSNDLLIGNFGDGTINAFDPATGESKGALTQANGSKVTQPGLWGLAFGNN
                     ADNQPSNTLFFAAGPTATTGVYGRIDSSM"
     gene            1635..1739
                     /locus_tag="VHV99_27705"
                     /pseudo
     CDS             1635..1739
                     /locus_tag="VHV99_27705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014725554.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
BASE COUNT          383 a          543 c          567 g          354 t
ORIGIN      
        1 cgcaatgtga acctcaggtg gctccaatac ggatcgcacg gaataaacac gcgtgcttga
       61 tcgttcaatg cgcaatgacg caacgccgac gcgaaggtcg cggggcaagg atgccatcga
      121 cgccgttcgc gggttggtgc gctcgttcag aacagaaaag ggtcgacgcg cgttgccagt
      181 ggtcgtgccg cgtggggact ataagcaaat cattcaagga tcaggaggtg acatgcatag
      241 gtttgcaact gttttctcgc ttgctggagc ggccacgctc gcacttgctg cgtgcggcgg
      301 caattcgaac aacaacatgc ccacggcgac cgccggtaca ttcaagtcga ccattctcgt
      361 gtccgacggc agcgtcgccg cgccgcacgt cgaccccaat ctgcaaaaca gctgggggat
      421 cgcgttcggt ccgaccaccc ccgtctgggt atcggacaac aatacacaga agtcgtcgct
      481 gtatgacggc aatggcgtgg tgcagtcgct cgtcgtcaca ctaccgccgg ccgcaagcgg
      541 tcaacccgcg agtccgaccg gcatcgtttt caacggtacc acggacttcg cagtaacggc
      601 gaatggcgca acaggcaacg cggcattcat ttttgccacc ctcgccggca cgatcgcggc
      661 ctggtcgcct aaggtgctgc cgacgcaggc tgtcaccgtt cacgatgacg gcgcaggcgc
      721 agccgaatac acaggccttg caatcgccag caacgccggc acaagcacgc tgtacgccgc
      781 cgatttccat aacggcaaag tcgatatgtt cgacaagact ttcacgaaag tttcggttgc
      841 cggcgccttc agcgatccga acgtacctgc gggttttaat ccgttcggca tccaggccgt
      901 cggcagtacg atttatgtgg cgtatgcgaa gctcgggccc gatggtcaca gagatcaaag
      961 cggtgcgggc aacggcgtgg tggatgcatt cgataccgcc ggccacctga tcaggcgcat
     1021 cgcgtcaaac gggccgctta attcgccgtg ggggatcgcc atggcgccgg ccaatttcgg
     1081 cgcgttcagc aacgacctac tgatcggcaa cttcggcgac ggcaccatca atgcgttcga
     1141 tcctgcgact ggcgagtcga aaggggcgct cacgcaggcc aatggttcaa aagtgacgca
     1201 gcccggactt tggggcctgg cgtttggcaa caacgccgac aaccagcctt ccaacacgct
     1261 gtttttcgcg gcaggtccga cggcgacgac gggtgtctat ggacgcatag attcgtcgat
     1321 gtagccggct gaaggtccaa gggctcgatg caatgtcgtc aagcgcggcg ccagagacgg
     1381 ctagctgtcc cgttcaagcg cctgtagcgg agcgcaaagc cggtgcgaac cgactttgtc
     1441 aacatgtcaa gattgaaacg gagcgcccgc cggtgggatt gtgatcctca tcgaaggtcc
     1501 gtatctctgc tataccgagc gcgtgccact tggtgtgtgt gcgctggcgt cgacccgaag
     1561 ttcgaactcg gccagcgcga agtgttcggg ccggtcaatg taagcatgcc gttcaccggc
     1621 gaggctgacg cattgccaac gatacgcagt tcggactcgc cgcatcggtg tggacgcagg
     1681 atgtggcgcg tacccaccgg gtggcgagta agctcatctt cggcatggtc cggattaacg
     1741 atggctgctg tgtcgtgata agcggtgagt ccaggctttt accttagaat aaggattcct
     1801 tatgtaacca acgagtagaa gtggtcggcg acaccgcgtt cgacgcc
//
LOCUS       DATASH010001045         8296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5720354,
            whole genome shotgun sequence.
ACCESSION   DATASH010001045 DATASH010000000
VERSION     DATASH010001045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 8296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8296
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5720354"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VHV99_27710"
     CDS             <1..621
                     /locus_tag="VHV99_27710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010895627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEX3639200.1"
                     /translation="KKVEELIARIAQKARAAGIHLILATQRPSVDVITGLIKANVPTR
                     MAFQVSSKIDSRTILDQQGAESLLGMGDMLYLPPGSGLPVRVHGAFVSDEEVHRVVDK
                     LKEQGEPNYIEGILEGGVTGEGDEGSAGAGGSGSGDGESDPLYDQAVDVVLKNRRASI
                     SLVQRHLRIGYNRAARLLEQMENSGVVSAMSSNGNREILAPAREAE"
     gene            854..1591
                     /gene="lolA"
                     /locus_tag="VHV99_27715"
     CDS             854..1591
                     /gene="lolA"
                     /locus_tag="VHV99_27715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HEX3639201.1"
                     /translation="MQLFAQQHARQSAQLSRFNQLARTIVRGVGSVVIGASMLVASQA
                     FASGTEQLKAFVAQVHSARGTFVQQEVRAPSKAQGASGASGVLSTAGKTGTSSGTFTF
                     ARPGKFIWQYEKPYAQLLQADGDKLYVYDKDLNQVTVRSLGGALGASPAAILFGSNDL
                     DKNFTLRDAGVKAGIDWLELTPKAKDTQFQRIGIGFKDGNLEAMELHDVFGNVTLLTF
                     SNIQKNPPLPADAFKFTVPKGADVING"
     gene            complement(1774..2694)
                     /locus_tag="VHV99_27720"
     CDS             complement(1774..2694)
                     /locus_tag="VHV99_27720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3639202.1"
                     /translation="MFSQLTDLDLRLIRVFLAIVDAGGVSPAQATLNVGQSTISTQLA
                     TLETRLGYRLCERGRGGFSLTARGEQFIDAARALLCAVDTFGMQARNVGRKLVGTLDV
                     GMIGHTPVSASARISDAIGRFRARDQSVRFSILVRSPGELEELLLNGRIQIGIGYFWH
                     RVPSLEYTEVFAEDQFAYCAKGHPLFAAAGDVSPAEAAVHEWAWRSYPLPEAESSTTP
                     QNVTAVADNMEAVAMLVLSGHHLGYLPGHFAAPFVKQGLLAALNPAQMRYRVAFHMVT
                     RARQHRTDIVEAFIDDMKAAHPVQVLEVDE"
     gene            2866..3855
                     /gene="speB"
                     /locus_tag="VHV99_27725"
     CDS             2866..3855
                     /gene="speB"
                     /locus_tag="VHV99_27725"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEX3639203.1"
                     /translation="MNTSAFISPEAGERPQPLSGNAMPRCGGIATMMRLPNVGSAEGF
                     DACFVGVPFDLGTSNRTGARFGPRQIRSESVLLRPYNMATRAAPFDSLRVADLGDVAI
                     NPYNLHDSIKRIEDAYDEILQHNCKPITLGGDHTIALPILRAIHRKHGKVGLIHVDAH
                     ADVNDTMMGEKIAHGTPFRRAVEEGLLDCERVVQIGLRGTGYAAEDFDWCRDQGFEVV
                     QAEACWNQSLVPLMARVRERMGNGPVYITFDIDGIDPAFAPGTGTPEIAGLTVPQALE
                     IIRGSHGLNIVGCDLVEVAPPYDPFGTTALLGANLAFELLCVLPGVEYRASKR"
     gene            complement(3949..5265)
                     /gene="cytX"
                     /locus_tag="VHV99_27730"
     CDS             complement(3949..5265)
                     /gene="cytX"
                     /locus_tag="VHV99_27730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HEX3639204.1"
                     /translation="MAQDPLAGAAGSTYAPLTPVPDARRAFRTGDAFALWFSLGIGLL
                     VAQAGALLVPGLSLPHALLAIVIGSVIGVVLLALAGVIGTDTGLAAMSSLRPTLGVRG
                     ASVPAVLNAVQLVGWGSFEVIVMRDSADALAKQAFGFSMPLIWTIVFGLLATLLAISG
                     PLSFVRRFLRTWGIWLLLAGAAWLSWNLLARHDLTALMHRPGTGEMSFGGAIDLVVAM
                     PLSWLPLIADYTRFGRRPGETFRGTLLGYGIANIWFYALGAIYGLAAGGGDALLTGAL
                     AQAGGGLALLLILIDEVDNAFADIHSAAVSTGTFWTRGSVPLLSAAFGALCTAIALLV
                     PMAKYQNFLLLIGSVFAPLFGVVLVDHFIVRKRRIEAAVLADIRGRYGFSGGWHLSAF
                     IAWAIGIVSYQAINQWLPNLGATLPALAIGAVCYLALVSTRKTAYA"
     gene            5664..6977
                     /locus_tag="VHV99_27735"
     CDS             5664..6977
                     /locus_tag="VHV99_27735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEX3639205.1"
                     /translation="MFEEPRANVPLAERLRPRNIDEVIGQKHLLGPNKPLRVAFESGE
                     AHSMILWGPPGVGKTTLARLMADAFHAEFIALSAVLSGVKDIREAVETAQIHRANGHQ
                     TLVFVDEVHRFNKSQQDAFLPHVESGLFVFVGATTENPSFEVNSALLSRAAVYVLKSL
                     TDEEQRELLERAQQELGGLTFTDEARDALIGSADGDGRKLLNNLEIVARAAAQQKTTG
                     IDGALLGSALAENLRRFDKGGDVFYDQISALHKSVRGSSPDGALYWFCRMLDGGADPR
                     YLARRIVRMAWEDIGLADPRAARIALDAAETYERLGTPEGELALAQAIIYLAVAPKSN
                     AGYNAYNEARRFVSKDQSRGVPVHLRNAPTKLMKELGYGHEYRYAHDEPDAYAAGETY
                     LPDNMREPHWYEPTPRGLEGKIGDKMARLAELDAQWRSENKPKKG"
     gene            7114..>8296
                     /gene="serS"
                     /locus_tag="VHV99_27740"
     CDS             7114..>8296
                     /gene="serS"
                     /locus_tag="VHV99_27740"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013338512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX3639206.1"
                     /translation="MLDIQLLRKDLDGVAKRLADRGYTLDVAAFTALEAERRDTQTRT
                     EEMQARRNTLSKQIGGMKGRGEDTSALMAEVGGIGDEMKASAARLEDIQKRLSDLLLG
                     VPNLPHASVPEGQDESGNVEVRRWGTPRQFDFEVKDHVDVGTPLGLDFETGAKLSGAR
                     FTLLRGQIARLHRALAQFMIDTHTLHHGYTEVYTPYIVNPEILYGTGQLPKFADDMFR
                     VEKGGDENTVTQYLISTSEISLTNTVRDSIVEADTLPIKLTAHSPCFRSEAGSYGRDT
                     RGLIRQHQFDKVEMVQIVAPEASYDALEQMVGHAETILQKLELPYRVITLCTGDMGFS
                     AAKTYDLEVWVPAQNTYREISSCSNTESFQASRMQARFRNAQGKPELVHTLNGSGLAV
                     GR"
BASE COUNT         1612 a         2628 c         2670 g         1386 t
ORIGIN      
        1 aagaaggtcg aggaactgat tgcgcggatc gcgcagaagg cgcgggcggc cggcatccat
       61 ctgattctcg ccacgcagcg gccatcggtc gatgtgatca ccgggctgat caaggccaac
      121 gtgccgacgc ggatggcgtt ccaggtgtcg tcgaagatcg actcgcgcac gattctcgac
      181 cagcagggcg cggaatcgct gctcggcatg ggcgacatgc tgtatctgcc gccgggcagc
      241 ggcttgccgg tgcgtgtgca tggcgcgttc gtgtcggacg aggaagtgca tcgcgtcgtc
      301 gacaaactca aggaacaggg cgagccgaac tatatcgagg gcattctcga aggcggcgtg
      361 acgggcgagg gcgacgaagg ctccgcgggc gccgggggaa gcggctcggg cgacggcgag
      421 tccgacccgt tatacgacca ggcggtcgac gtggtgctga agaaccgccg agcgtcgatc
      481 tcgctggtgc agcggcattt gcgcatcggc tataaccgcg cggcgcgtct gctcgagcag
      541 atggagaact cgggcgtggt gtcggcgatg tcgtcgaacg gtaatcgcga gattcttgcg
      601 ccggcacgtg aagcggaata aggcgccacg cggcgctaat accgcggcgc ggaacgtgct
      661 tgctgctacg agcggcgccg cgaaacgcag catctaaggc ccatttaagg ctcggcgctt
      721 aagctgctca tgatgagccg cggtcgcgcc cgtccgccac gcggttcaac tgagcgccgc
      781 ggcccgcgca cccgctgtgc tattcgccgg ccgccgcaat cgctcgacac aatcaatcaa
      841 gggagaaaac accatgcagc ttttcgcgca gcagcacgct cgacagagtg ctcaactcag
      901 ccgcttcaac caactcgctc gcacgatcgt acgtggcgtc ggcagtgtgg tgatcggtgc
      961 gtcgatgctc gtcgcgtcgc aggcgttcgc gagcggcacc gagcaattga aggcattcgt
     1021 cgcgcaagtg cattccgcgc gcggtacctt tgtgcagcag gaagtgcgcg cgccgagcaa
     1081 ggcgcagggc gcaagcggcg cgagcggcgt gttgtccacg gccggcaaga ccggcacgtc
     1141 gagcggcacg ttcacgttcg cccgtcccgg caagttcatc tggcaatacg agaagcccta
     1201 tgcacaactg ctgcaagccg acggcgacaa gctctacgtg tatgacaagg atctgaacca
     1261 ggtgacggtg cgcagcctcg gcggcgcgct cggtgcgagc ccggcggcga tcctgttcgg
     1321 cagcaacgat ctggacaaga atttcacgct gcgcgatgcg ggcgtgaagg ccggcatcga
     1381 ctggctcgaa ctgacgccca aggcgaagga cacgcagttt cagcgcatcg gcatcggctt
     1441 caaggacggc aatcttgaag cgatggaact gcatgacgta ttcggtaacg tgacgttgtt
     1501 gacgttctcg aacattcaaa agaacccgcc gctgccggcc gacgcgttca agttcacggt
     1561 gcccaagggc gcggatgtga tcaatgggtg agcgggttgt cgcaccaggt tgatttgcag
     1621 aaaggctgcg catcagcgcg gccattttca tgtcgatcga ttgccgatcg cgctgcacac
     1681 ccgacgcatc gttcgttctt ttcaggacct gagctggctg tgcagacctg ctgcttcgtc
     1741 tgttttcaga acgtaggcga tcaggctgaa ccgctactca tccacctcca gaacctgcac
     1801 cggatgtgcg gccttcatat cgtcgataaa cgcttcaacg atatccgtcc gatgctgccg
     1861 ggcccgcgtg accatatgaa acgccacgcg ataccgcatc tgtgcgggat tcaacgcggc
     1921 caacaagccc tgcttgacga acggcgccgc gaaatgtccc ggtaaatagc cgagatgatg
     1981 ccccgacagc accagcatcg cgaccgcttc catgttgtcc gcgacggcgg tgacgttctg
     2041 cggcgtagtc gaactttcgg cctcgggcaa cggatagctg cgccaggccc actcatgaac
     2101 ggcagcttca gcaggtgaca cgtctccggc tgcagcgaac agcggatgcc ccttcgcgca
     2161 atacgcgaac tgatcttcgg cgaacacctc ggtgtattcg agcgacggca cgcgatgcca
     2221 gaagtagccg atgccgatct gaatccgccc gttcagcaac agctcttcca gctcgcccgg
     2281 cgagcgcacc agaatcgaaa accgcaccga ctgatcgcgc gcccgaaacc ggccgatcgc
     2341 gtcgctgatg cgcgcgctcg ccgacaccgg cgtatggccg atcatgccga catcgagcgt
     2401 gccgaccagc ttgcggccga cattgcgcgc ctgcatgccg aaggtgtcga cggcgcagag
     2461 gagggcacgg gccgcgtcga tgaattgttc gccgcgcgcg gtcaggctga agccgccgcg
     2521 gccgcgttcg cacagccggt aaccgaggcg cgtttccagc gtggcgagct gcgtgctgat
     2581 ggtcgactgg ccgacgttga gggtggcctg ggccggcgag acgccgccgg cgtcgacgat
     2641 agcgaggaag acgcggatta gccgcaggtc gagatcggtg agttgggaga acatacgtca
     2701 ggatacatcg ctgcaaatcg atgtgaagtt tattctgttc gtattttaac ttgcggaaaa
     2761 ctgctcaaga attgagattc ccccggcaag gtgcgctttt tcattagacc tctcagccgg
     2821 ttgcactcca ttgggctagc gccccctttt cattgccgag acacgatgaa tacttccgcc
     2881 ttcatttccc ccgaggccgg cgaacggccg cagccgttgt ccggcaatgc catgccccgt
     2941 tgtggcggca tcgcgaccat gatgcggctg ccgaacgtcg gctcggccga agggttcgac
     3001 gcgtgcttcg tcggcgtacc gttcgatctc ggcacgtcga accgcaccgg cgcgcgcttt
     3061 gggccgcgcc agatccgcag cgaatcggtg ctgctgcgcc cgtacaacat ggcaacgcgc
     3121 gccgcgccgt tcgattcgct gcgcgtggcc gatctcggcg acgtcgcgat caatccgtac
     3181 aacctgcacg attcgatcaa gcgcatcgaa gacgcctacg acgaaatcct ccagcacaac
     3241 tgcaagccga tcacgctcgg cggcgaccac acgatcgcgc tgccgatcct gcgcgcgatc
     3301 catcgcaagc acggcaaggt cggtctgatt cacgtggatg cgcacgccga cgtcaacgac
     3361 acgatgatgg gcgagaagat cgcgcacggc acgccattcc gtcgcgcggt ggaagagggc
     3421 ttgctcgatt gcgagcgtgt cgtgcagatc ggcctgcgtg gcacgggtta cgcggccgaa
     3481 gatttcgact ggtgccgcga tcagggtttc gaggtcgtgc aggcagaggc gtgctggaac
     3541 cagtcgctcg tgccgctgat ggcgcgcgtg cgggaacgca tgggcaatgg tccggtgtac
     3601 atcacgttcg acatcgacgg gatcgatccg gccttcgcgc cgggtacggg cacgccggaa
     3661 atcgcggggt tgacggtgcc gcaggcgctc gaaatcattc gcggctcgca tgggttgaac
     3721 atcgtcggct gcgatctggt tgaggtggcg ccgccgtacg atccgttcgg cacgaccgcg
     3781 ctgctcggcg cgaatctcgc gttcgaattg ctgtgtgtgt tgccgggcgt cgaatatcgg
     3841 gcgtcgaagc gttgaagtgc gggttgaact gaccaggttt gatgacgcat gaagagaaaa
     3901 ggcctgcatt tgcaggcctt ttccgtattg cggtgatgct tgctccgctt acgcgtacgc
     3961 agttttccga gtcgacacca gcgccagata acacaccgcg ccgatcgcca acgcgggcaa
     4021 cgtagcgccc agattcggca gccattgatt gatcgcctga tacgacacga tcccgatggc
     4081 ccacgcgata aacgcgctga gatgccagcc gcccgagaag ccataacggc cgcgaatgtc
     4141 ggcgagtacg gcggcttcaa tgcgacgctt gcgcacgatg aagtgatcca ccagcaccac
     4201 gccgaacagc ggcgcgaaca ccgagccgat cagcagcagg aagttctgat acttcgccat
     4261 cggcacgagc aaggcgatcg ccgtgcacag cgcgccgaat gccgccgaca gcaacggtac
     4321 actgccgcgc gtccagaacg tgccggtcga aaccgctgcc gagtggatat cggcgaacgc
     4381 gttgtcgact tcgtcgatca ggatcagtag cagggcgagg ccgccgcccg cttgagccag
     4441 cgcgccggtc aggagcgcat cgccaccgcc cgccgcgaga ccgtagatcg cgccgagcgc
     4501 gtaaaaccag atgttcgcaa tgccataacc cagcagcgtg ccgcggaagg tttcgcctgg
     4561 gcgtcggccg aaacgcgtgt agtcggcgat cagcggcagc catgacagcg gcatcgcgac
     4621 caccagatcg atagcgccgc cgaacgacat ctcgccggtt cccggacggt gcatcagcgc
     4681 ggtcagatcg tgcctggcca gcagattcca cgagagccac gcggcgccgg ccagcagcag
     4741 ccagatgccc catgtgcgca gaaaccgtcg cacgaacgac agcgggccgc tgattgccag
     4801 cagcgtcgcg agcaggccga agacgatcgt ccagatcagc ggcatcgaga acccgaaggc
     4861 ctgtttggcg agcgcatcgg cggaatcgcg catcacgatc acctcgaacg agccccagcc
     4921 gaccagttgc accgcgttca gcaccgccgg caccgaggcg ccacgcacac cgagcgtcgg
     4981 gcgcagcgac gacatcgccg cgagccccgt atccgtgccg attacgccgg ccagcgccag
     5041 caatacgacg ccgatcacgc tgccgatcac gatcgccagc aaggcatgcg gcagcgacag
     5101 ccccggcact agcagcgcgc ccgcctgcgc gaccagcagg ccaatgccga gcgagaacca
     5161 gagcgcgaaa gcgtcgccgg tgcgaaacgc gcggcgcgcg tcgggcaccg gcgtgagcgg
     5221 tgcgtaggtc gagccggcgg cgccggcgag tggatcttgt gccatttaat tgtgttcctg
     5281 ttgatccgag ttagcgtgtt ggctggagtt ggcgtgtggg ttcgagctgg cgtggcggct
     5341 gaagttggcg ctttagcgct tactcgggcc acatagcgct tactagagcc ccatggcacg
     5401 ctactctggt tccatagcgc ttactccagc ccaatgcaac atagttgcgg ttgatattgc
     5461 tagcagatcc gcgtgcgacg ccggcgttgc cggtcaatcc gcgcgcgacc gtcgagccgc
     5521 cgcgcggccg tcacccaggc gccgtcactg tcataatgca gcacgttgcc cgcgccgccg
     5581 tgcgccggct ttgcccgtcc ggcagcacgc cgccttcaca cggaccgcgt ccggaaaagc
     5641 cgagattatc cccaaaaaac gtcatgtttg aagaaccccg tgccaatgtt ccgctcgccg
     5701 aacgcctgcg gccacgcaat atcgacgaag tgatcggtca gaagcacctg ctcggcccga
     5761 acaagccgtt gcgggtcgcc ttcgaatccg gcgaagcgca ttcgatgatc ctgtgggggc
     5821 cgcccggcgt cggcaaaacc acgctcgcgc ggctcatggc cgacgcgttt cacgccgagt
     5881 tcattgcatt gtccgccgtg ctttctggcg tgaaggatat ccgcgaagcc gtcgagacgg
     5941 cgcagattca tcgtgccaac gggcaccaga cgctcgtgtt cgtcgacgaa gtgcatcgct
     6001 tcaacaagag tcagcaggac gcgttcttgc cgcacgtcga gtcggggctg ttcgtgttcg
     6061 ttggcgcgac gaccgaaaat ccatcgttcg aggtgaacag cgcgttgctc tcgcgagccg
     6121 ccgtctacgt gctgaaaagc ctcaccgacg aagaacagcg cgaactgctc gagcgcgccc
     6181 agcaagaact cggcggcctc acgttcaccg acgaagcacg cgacgccctg atcggctcgg
     6241 ccgacggcga cggccgcaag ctgctgaaca acctcgagat cgtcgcccgc gcggctgcgc
     6301 agcagaaaac caccgggatc gatggcgcgt tgctcggtag cgctctggca gaaaatctgc
     6361 gccgcttcga caaaggcggc gacgtattct acgaccagat cagcgcgttg cataaatcgg
     6421 tgcgcggcag cagcccggac ggcgcgctgt actggttctg ccgcatgctc gacggcggcg
     6481 cggacccgcg ttatctcgcg cggcgtatcg tgcgcatggc gtgggaagac attggccttg
     6541 ccgacccgcg cgcggcccgc atcgcgctcg acgccgccga aacctacgag cgcctcggca
     6601 cgcccgaagg cgaactggcg ctggcgcagg cgatcatcta tctggcggtc gcgccgaagt
     6661 cgaacgcggg ttacaacgcg tacaacgagg cacgccgttt cgtcagcaaa gaccagtcgc
     6721 gtggcgtgcc ggtgcatctg cgtaacgcgc cgaccaagct gatgaaggaa ctcggctacg
     6781 gtcacgaata ccgctacgcg cacgacgagc ccgatgccta cgcggccggc gaaacctatc
     6841 tgccggacaa catgcgtgaa ccgcattggt acgagccgac gccgcgcggt ctcgaaggca
     6901 agatcggcga caagatggcg cgtctggccg agcttgacgc gcagtggcgc agcgagaaca
     6961 agcctaaaaa gggctgagtg tctgcgcttg tgctgccgcg gccgtttcgg atgctgtatc
     7021 gccattgcgc ggcccttaac gatctaacac accggccgcc cgtgccggtg cgctaaaatc
     7081 cctgcttcat acaacgaata cccgccccat cccatgctcg acattcagct gctgcgcaaa
     7141 gacctcgacg gcgtcgccaa gcgcctcgcc gaccgcggct acaccctcga cgtcgcggcc
     7201 tttaccgcgc tcgaagcgga acgccgcgac acccagaccc gtaccgaaga gatgcaggcg
     7261 cgccgcaaca ctttgtcgaa gcagatcggc gggatgaagg ggcggggcga ggatacgtcg
     7321 gcgctgatgg ccgaagtggg cggtatcggc gacgagatga aagcgtcggc ggccaggctg
     7381 gaggatatcc agaagcgtct gtccgacctg ctgctcggcg tgcccaacct gccgcacgca
     7441 agcgtgccgg agggccagga cgaaagcggg aacgtcgagg tgcgccgctg gggcacgccg
     7501 cgccaattcg atttcgaggt gaaggatcac gtcgacgtcg gcacgccgct cggcctcgat
     7561 ttcgagaccg gcgcgaaact gtcgggcgcg cgtttcacgt tgctgcgcgg acagattgcg
     7621 cggctgcatc gcgcgctggc gcagttcatg atcgacacgc acacgctgca ccacggctat
     7681 accgaggtgt acacgccgta catcgtgaat ccggagattc tgtacggcac cggccaactg
     7741 ccgaaatttg ccgacgacat gttccgggtc gagaagggcg gtgacgagaa taccgtcacg
     7801 caatatctga tttcgacgtc ggagatttcg ctgacgaaca cggtgcgcga cagcatcgtc
     7861 gaagcggaca cgctgccgat caagctgacc gcgcattcgc cgtgcttccg ctcggaagcg
     7921 ggctcatacg gccgcgatac gcgcggcctg atccgccagc atcagttcga caaggtcgaa
     7981 atggtgcaga tcgtcgcgcc ggaagcttca tatgacgcgc tggaacagat ggtcggccac
     8041 gctgagacga tcctgcagaa gcttgaactg ccgtaccgcg tgattacgct gtgcacgggc
     8101 gacatgggct tttcggctgc caagacgtat gacctggaag tgtgggtgcc cgcgcagaat
     8161 acatatcgcg agatttcgag ctgctcgaac acggagtcgt tccaggctag ccgcatgcag
     8221 gcgcgtttcc gcaatgcgca aggcaagccg gagctggtgc atacgctgaa cggatcgggt
     8281 ctggcggttg gccgca
//
LOCUS       DATASH010001046         1949 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1170131,
            whole genome shotgun sequence.
ACCESSION   DATASH010001046 DATASH010000000
VERSION     DATASH010001046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1170131"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /gene="fliG"
                     /locus_tag="VHV99_27745"
     CDS             complement(<1..885)
                     /gene="fliG"
                     /locus_tag="VHV99_27745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017334572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HEX3639207.1"
                     /translation="MSAEGVMKSALLLMSIGEEEAAQVFKFLGPREVQKIGVAMAALK
                     SVTREQVDEVLQEFVREAETHTGMSLDSSEYIRSVLTKALGDDKAGAIIDRILQGSDT
                     SGIEGLKWMDSAAVAELIKNEHPQIIATILVHLDRDQASEIVACFTERLRNDVLLRIA
                     TLDGIQPAALRELDDVLTGLLSGSDNLKRSPMGGIRTAAEILNFMSSNHEEGVIENVR
                     QYDAELAQKIIDQMFVFENLLDLEDRAIQLLLKEVESEALIISLKGAPPALRQKFLSN
                     MSQRAAELLAEDLDARGPV"
     gene            complement(875..>1949)
                     /gene="fliF"
                     /locus_tag="VHV99_27750"
     CDS             complement(875..>1949)
                     /gene="fliF"
                     /locus_tag="VHV99_27750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006753020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HEX3639208.1"
                     /translation="VADMPVKGVTILDQDGNLLTQSSTGSGLDASQLKYRQQIERNTQ
                     QRIDAILEPLFGSGNAHSQVSADIDFSRSEQTAENYGPNGNPQQAAIRSQQSSTATEM
                     SQAGASGVPGALSNQPPQPASAPITAASGTAGVTTTPVSDRKDSTTNYELNKTVQHLE
                     QPMGGIKRLSVAVVVNYQRVVDARGHATMQPVTADKLAQVNQLVKDAMGFDATRGDSV
                     NVVNSPFTADVDPVADLPWWRTPDMIALYRQIATYLGIGAVALFLYFVMVKPALRRAF
                     PPPEPVAAALSSPGDEPILLDGIPAAEREGASVQLETDSEVLALESAKQKYERNLEFA
                     RNIARQDPKIVATVVKNWVSDER"
BASE COUNT          299 a          616 c          631 g          403 t
ORIGIN      
        1 caccgggccg cgcgcatcga ggtcttcggc gagcagttcg gcagcacgct gcgacatgtt
       61 cgacaggaac ttctggcgca gcgcgggcgg cgcgcccttc agcgagatga tcaacgcctc
      121 ggactcgact tccttcagca acagctgaat cgcgcggtct tccagatcga gcaggttctc
      181 gaacacaaac atctgatcaa tgatcttctg cgcgagttcc gcgtcgtatt ggcgaacgtt
      241 ctcgatgacg ccttcttcat gattgctcga catgaagttg agaatttcgg ccgccgtgcg
      301 gatgccgccc atcgggctgc gcttcaggtt gtcgctgccg gacaggaggc ccgtcagcac
      361 gtcgtcgagt tcgcgcagcg cggccggctg gatgccgtcg agcgtggcga ttcgcagcag
      421 cacgtcgtta cgcagccgtt cagtgaagca ggcgacgatt tccgaagcct gatcgcggtc
      481 caggtgaacg aggatggtcg cgatgatctg cggatgctcg tttttgatga gctcggccac
      541 cgcagccgag tccatccact tgaggccttc gatcccgctg gtatcgctgc cctgcagaat
      601 ccggtcgatg atcgcgccgg ccttgtcgtc gccaagcgcc ttggtcagca ccgagcggat
      661 gtattcgctc gaatccagcg acatgcccgt gtgcgtttcc gcctcgcgga cgaattcctg
      721 caagacctcg tcgacctgct cgcgcgtcac gcttttcagg gcggccatgg cgacgccgat
      781 cttctgaacc tcacgtggcc cgaggaactt gaacacctgc gcggcttctt cctcgccgat
      841 cgacatcagc aggagtgcgc tcttcattac gccttcagcg ctcatcggac acccaatttt
      901 tcacgacggt tgcgacgatt ttcggatcct ggcgcgcgat gttgcgcgcg aactccaggt
      961 tccgttcgta tttctgtttg gcgctttcga gcgcgagcac ttcgctgtcc gtttccagtt
     1021 ggacacttgc gccttcgcgc tcggcggctg gaatgccgtc gagcaggatc ggttcgtcgc
     1081 ccggcgagga cagcgcggcg gcgaccggct ccggcggcgg gaaagcgcga cgcagcgccg
     1141 gcttcaccat cacgaagtag aggaacaggg ccactgcgcc gatgccgagg taggtcgcaa
     1201 tctgcctgta gagcgcgatc atgtccggcg tgcgccacca cggcaggtcg gcgacggggt
     1261 cgacgtcggc ggtgaacggg ctgttgacca cgttgaccga gtcgccgcgc gtggcgtcga
     1321 agcccatcgc gtccttcacc agttgattga cctgagcgag cttgtcggcg gtgaccggtt
     1381 gcatcgtcgc gtggcccctg gcgtcgacta cccgctgata gttgaccacc acggccaccg
     1441 acagacgctt gatgccgccc atcggttgtt ccagatgctg cacggtcttg ttgagctcgt
     1501 aattggtggt cgaatccttg cggtcgctga ccggcgtggt cgtcacaccg gccgtgccgc
     1561 tggccgccgt gatcggtgcg gaagcaggct gcggcggctg gttcgacagc gcgcccggca
     1621 cgcccgaagc gccggcttgc gacatctcgg tggcagtgct cgactgctgg ctgcggatcg
     1681 ccgcctgctg cgggttgccg ttcggaccgt agttttcggc ggtctgctcg ctgcgcgaga
     1741 agtcgatgtc ggcgctgacc tgcgagtgcg cgttgccgga gccgaacagg ggttccagga
     1801 tcgcgtcgat gcgctgttgc gtgttgcgtt cgatctgctg acggtacttc agttgcgagg
     1861 cgtccagacc gctgccggtg gacgactgcg tcagcagatt accgtcctgg tcgaggatgg
     1921 tcacgccctt caccggcatg tccgccacc
//
LOCUS       DATASH010001047         7010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1170323,
            whole genome shotgun sequence.
ACCESSION   DATASH010001047 DATASH010000000
VERSION     DATASH010001047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7010
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1170323"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="VHV99_27755"
     CDS             complement(<1..564)
                     /locus_tag="VHV99_27755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3639209.1"
                     /translation="MQPIVSVTNLSKTYATGFHALKNINLAINRGEIFALLGPNGAGK
                     TSLISIICGIVKASEGSVMVDGRDIAVDYRGARSLIGLVPQELTTDSFETVWATVSFS
                     RGLFGKPKNPAYIEKVLRDLSLWEKRDSKIITLSGGMKRRVLIAKALSHEPRVLFLDE
                     PTAGVDVELRRDMWKLVRSLAASRVTII"
     gene            complement(850..2769)
                     /locus_tag="VHV99_27760"
     CDS             complement(850..2769)
                     /locus_tag="VHV99_27760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3639210.1"
                     /translation="MSDATNPLILNVDDNDGARYAKSRILSRAGFVVIEAANGTEAIE
                     RARLSLPDLVLLDVKLPDVNGFEVCRQIKSAPETAGILVLQTSAAAVQSIDKIRGLDG
                     GADSYLIEPIEPAELIAHVRALLRVRRAEGALRESEERFRQMAENIDDVFWMLDPSTD
                     QLLYVSPAYRRLWGDEAAAPHPGAGHWAGRVHPQDEAAVGSAYRALATGDPYEIEYRI
                     VRPDGETRWVAERGFPVRDGRDRIYRLAGIVNDISERKANEMVLRDADRRKDEFLAML
                     AHELRNPLAPIRNAIELLDPSRSPSPQNFAAMRAVIGRQVKHLSRLVDDLLDVARITQ
                     GKITLRQEIVGLAAAIEAAIETAAPTLTRKGHTLRTEMPDEPLFIVGDGVRIAQVLGN
                     ILSNAAKYTPQNGEILIDVRELSDNVQITVRDNGVGIAADTIPHIFDLFVQSESSLER
                     SEGGLGIGLPLARTLVELHGGTIEAFSAGPGKGSEFIVCLPLTRLAQTDRPLVSAPVT
                     VPHGAGPALRLLLVDDSVDAATAMSLLLESDGYDVRIAHEAEHALEIAAQFEPEIVLL
                     DLGLPGMDGFQLAQEMRKRAATANALLIAVTGYGQAADRQRSHEAGFDHHLVKPVSSD
                     EIHRVIASRFPCGHS"
     gene            complement(2766..4145)
                     /locus_tag="VHV99_27765"
     CDS             complement(2766..4145)
                     /locus_tag="VHV99_27765"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX3639211.1"
                     /translation="MAEMLHAMPIDSEFAMVAARRRARELGEALAFTAHDQTRIATSV
                     LEVARTVLSATPHGRAAFLLDETSRPPALLIRFVAPATTFDQLRGRTAGTARPTDAEE
                     PAGVMGVLAAQRLMDACRFEDVAGDAVVTLIKHLPEQGSRPTAAAVAGVAALAEGAAA
                     SGAQGSFDEVQRQNRELVNALYDLRERQEELVRLTRELEDTNRGVVALYAELDERADH
                     LRRTDEAKSRFLSNMSHEFRSPLYSIRALSKLLMDRADGELTDEQAKQVRFIRKAAEE
                     LSETVDDLLDIAKIEAGKIELRPVEFEVANLFSALRGMLRPLVPASGVELLFDPCDDI
                     PPVCADEGKVSQILRNFITNALKFTERGEVRVRAQFDAAQQRLTFSVSDTGIGIAPEH
                     QQSVFEEFEQVENRLQTYVKGTGLGLPLCSKLCKLLGGSVGLQSELGKGSTFTATIQA
                     YVPTENTLP"
     gene            complement(4145..5215)
                     /locus_tag="VHV99_27770"
     CDS             complement(4145..5215)
                     /locus_tag="VHV99_27770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma regulatory factor"
                     /protein_id="HEX3639212.1"
                     /translation="MEAVLNGLSATQQRFEIGDPSQVSYARRGISELAHRTGCSETVI
                     GRLAIIVTECATNQLKHAQRGELLVRPISEALPSRYGIELLALDSGPGIHDLHACFVD
                     GYTTAGSPGNGMGAIQRLADELDIWSAPGLGTVLRIVVWAEPGSADAEPCGPIEYGAI
                     NLPLQGETLCGDAWTCGYMEGEFTVMVADGLGHGALANTAAVAATATFARLGPSSVEE
                     IVAASHEALRPTRGAALGVARIPAQAVANGAPGVARFCGVGNIAASVWTPYGHRHLVS
                     HAGIVGHQLRKVQEFQVDWPQDGLLILHSDGLATRWDLSRYPGLTLRHPTLVAATLYR
                     DFTRGRDDVTVFVARAHHAELL"
     gene            complement(5200..5586)
                     /locus_tag="VHV99_27775"
     CDS             complement(5200..5586)
                     /locus_tag="VHV99_27775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008354418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma regulatory factor"
                     /protein_id="HEX3639213.1"
                     /translation="MPIRSDEQIVRLRRFVREKAVALGMSLIDQTKFVTAASELARNT
                     LTYGGGGDVHFYIVQRDGRTGLRLEFVDTGPGIADIQKALTDGYTSGNGLGLGLGGAK
                     RLCDEFEIRSAPGEGTHISVTKWKPF"
     gene            complement(5703..6062)
                     /locus_tag="VHV99_27780"
     CDS             complement(5703..6062)
                     /locus_tag="VHV99_27780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007582301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX3639214.1"
                     /translation="MERIPILRMGKLLLVTIQVDMHDRLAMTLQDDLTSRIVKDRAKG
                     VLIDISSLDVVDSFIGRMIGDTAAMARVLDAQTVVVGMQPSVAITLVELGLALPGVRT
                     ALNVEKGMALLSGTEPL"
     gene            complement(6097..>7010)
                     /locus_tag="VHV99_27785"
     CDS             complement(6097..>7010)
                     /locus_tag="VHV99_27785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX3639215.1"
                     /translation="GTRLAQLFNERQSELLGEWVAQQYAIAARRGGVTEAQLRNQFVQ
                     FVGLTCAALGASDRVEFKTPAWDDVRGLLAEVSTQRARQGFTPVETAMFVFSLKEPLF
                     ARLRSELANDPATLAELSWTIGSLFDALGLYTMEVFQTSREQVIMRQQQELLELSTPV
                     VQLWEGILALPLIGTLDSARTQVVMESLLQKIVETGAAIAIIDITGVPTVDTLVAQHL
                     LKTVAAARLMGADCIISGIRPQIAQTIVHLGVNLSNVITKATLADAFVIALQRTGRSL
                     SNGDRHAEAPGHSGSPAALRGNPFAGD"
BASE COUNT         1286 a         2238 c         2229 g         1257 t
ORIGIN      
        1 gatgatcgtc acgcggctcg cggcgagcga gcgcaccagc ttccacatgt cgcggcgcaa
       61 ttcgacgtcc acaccggccg tgggttcgtc gagaaacagc acgcgcggtt cgtgcgagag
      121 cgccttcgca atcagcacgc ggcgcttcat gccgcccgac agcgtaatga tcttgctatc
      181 gcgcttctcc cacaacgaca gatcccgcag caccttttcg atgtaagcag ggttcttcgg
      241 cttgccgaac agcccgcggc tgaacgagac ggttgcccag acggtttcga acgagtcggt
      301 ggtgagttcc tgcggcacga ggccgatcag cgagcgcgcg ccacgatagt ccaccgcgat
      361 atcgcggccg tccaccatca cgctgccctc gctcgccttg acgatgccgc agatgatgct
      421 gataagggaa gttttgccgg cgccgttcgg gcctaacaaa gcgaagattt ctccgcggtt
      481 gatcgccaga ttgatgtttt tgagtgcatg aaaacccgtg gcataggttt tcgacagatt
      541 cgtgaccgag acgattggct gcatggattc gacgatggca gacaccgtta tttgattgga
      601 aggaagcggt gcgaatgcgc gaccgcaccc gcaatgtaat ggaaagtgcg aaagcgtgca
      661 gcatgccatc gaattacggc gaacggaggc gcctgccagg gtcctggctg aatcccgcgc
      721 gcacggcgca gaaaaaaaga aagccggcta tttcgccgca atgcaaaata gttcggcttc
      781 catatcgatg cagtgaaggc cggcgccccg gcgccaccat gtccatgccg ggcggcgtgc
      841 aactacggct taactatgcc cacacgggaa acggctggca atcacgcggt gaatttcatc
      901 ggacgacacc ggcttcacca gatgatgatc gaagcccgct tcgtgcgaac gctgacggtc
      961 ggccgcctgt ccataaccgg tgaccgcgat cagcagcgca tttgccgtag ccgcgcgctt
     1021 gcgcatttcc tgcgcgagtt gaaacccgtc catgccgggc aagccgagat cgagcaaaac
     1081 gatctctggt tcgaattgcg cggcgatctc gagcgcatgt tcggcttcat gcgcgatgcg
     1141 cacgtcataa ccgtccgatt cgagcaggag cgacatggct gtcgcggcat cgacgctatc
     1201 gtccaccagc agcaggcgca gcgccggacc cgcgccgtgc ggcacggtta caggcgcgct
     1261 gacgagcgga cgatcggttt gcgccaggcg cgtcagcggc aggcacacga tgaattcgct
     1321 gcccttcccc gggcccgccg aaaatgcctc gattgtgccg ccatgcaact cgaccagcgt
     1381 gcgcgccaat ggcaagccga tgccgaggcc gccctcggag cgttcgagcg aactctcgga
     1441 ctgcacgaac aggtcgaaga tatgcggaat ggtgtcggca gcaatgccga cgccgttatc
     1501 gcgcacggtg atctgcacgt tgtcgctcaa ctcgcggaca tcgatcagaa tctcgccgtt
     1561 ttgcggcgtg tacttggccg cgttcgagag gatattgccg agcacctgcg cgatccgcac
     1621 gccgtcgccg acgataaaaa gcggctcgtc aggcatctcg gtcctgagcg tgtggccctt
     1681 cctggtgagc gtgggcgcgg ctgtttcaat cgccgcctcg atggcggcgg caaggccgac
     1741 gatctcctgg cgcagcgtga tcttgccttg cgtgatccgc gccacgtcga gcagatcgtc
     1801 gacgaggcgg ctcaaatgct tgacctggcg gccgatcacc gcgcgcatcg cagcgaagtt
     1861 ctgcggcgaa ggcgagcggc tcggatcgag caattcgatg gcattgcgga tcggcgccaa
     1921 cggattgcgc agttcgtggg cgagcatcgc aaggaactcg tccttgcggc gatcggcgtc
     1981 gcgcagcacc atttcattgg ctttgcgctc cgagatatcg ttgacgatac cggccaaacg
     2041 gtagatacgg tcgcgcccat cgcgcaccgg aaagccgcgc tcggcgaccc agcgggtttc
     2101 accgtcgggc cgcacgatgc ggtactcgat ctcatacgga tcgcccgttg ccagcgcgcg
     2161 atacgcgctg ccgaccgcgg cttcgtcttg cggatggacc cggcccgccc agtggcccgc
     2221 gccgggatgc ggcgcggctg cctcgtcgcc ccatagccgc cgataggcgg ggctgacgta
     2281 cagcaactga tcggttgaag ggtcgagcat ccagaacacg tcgtcgatat tttcggccat
     2341 ctggcgaaag cgctcttcgc tttcacgcag ggcgccttcc gcgcgacgca cccgcaacag
     2401 ggcccgcaca tgcgcgatca gctcggccgg ttcgatcggc tcgatcaggt agctatcggc
     2461 gccgccgtcg agaccgcgaa tcttgtcgat gctctgcacg gccgccgccg aggtctgcaa
     2521 cacgagaatg ccggcggtct caggtgccga cttgatctga cgacacactt cgaagccgtt
     2581 gacatccggc agcttgacgt cgagcaacac gaggtcgggc aacgagagcc gcgcgcgttc
     2641 gatcgcctcg gtgccgttgg cggcttcgat cacgacgaag cccgcacgcg acagaatgcg
     2701 gctcttggca taccgtgcgc cgtcgttgtc gtcgacgttc aatatcaggg ggttggtcgc
     2761 gtcgctcatg ggagagtgtt ttccgtcggc acataagcct ggatggtcgc ggtaaaggta
     2821 gagcctttgc ccagttcgct ctggagccca accgaaccgc ccagcaactt gcagagcttg
     2881 ctgcacagcg gcagaccgag ccccgtgcct ttcacatagg tctgcaggcg gttttccacc
     2941 tgctcgaatt cttcgaatac gctttgctgg tgctcgggcg caatgccgat gccggtatcg
     3001 ctcaccgaga aggtcaggcg ctgttgagcg gcgtcgaatt gggcccgcac ccgtacctca
     3061 ccccgttcgg tgaactttag cgcattggtt atgaaattgc ggagaatctg cgagaccttg
     3121 ccttcgtcgg cgcacaccgg cgggatgtcg tcgcaaggat cgaacagcaa ttcgacaccg
     3181 ctcgccggca ctagcggacg caacatgccg cgcaaggccg aaaacagatt ggcgacttcg
     3241 aattcgaccg gccgcagttc aatcttgccc gcctcgatct tcgcgatatc gagcaggtcg
     3301 tcgacggttt cggacaactc ctcggctgct ttgcggatga agcgcacctg tttggcttgt
     3361 tcgtcggtca gctcgccatc ggcgcggtcc atcagcagct tcgagagcgc gcgaatcgaa
     3421 tacaggggcg agcggaattc atggctcata ttcgacagga agcgcgactt cgcttcgtcg
     3481 gtacggcgca ggtggtcggc gcgctcgtcc agttcagcgt agagcgcgac cacgccgcga
     3541 ttggtgtctt caagctcgcg cgtgagccgg accagttctt cctgacgttc ccgcagatca
     3601 taaagcgcgt tgaccagctc gcggttttgc cgctggactt cgtcgaacga accttgcgcg
     3661 cccgaggcgg ccgccccttc ggcaagcgcg gcgacgccag caacggcggc ggcggtagga
     3721 cgcgacccct gctccggcaa gtgcttgatc aaggtcacga cggcgtcgcc cgcgacgtct
     3781 tcgaaccggc acgcatccat cagacgctgg gcagcgagca cgcccatgac gccggccggt
     3841 tcttcggcat cggtgggtcg agcggtgccg gcggtccgcc cgcggagctg atcgaacgtg
     3901 gttgcgggtg caacgaaacg aatcagcaag gctggcggac gactcgtttc gtcgagcagg
     3961 aatgcggcgc gcccgtgcgg cgtggcgctc agcacggtac gcgcgacttc aagcaccgag
     4021 gtggcaatgc gtgtttgatc gtgcgcggtg aaggcaagcg cttcgccaag ttcacgcgcg
     4081 cgccggcgcg cggcaaccat ggcgaactcg ctgtcgatcg gcatcgcatg gagcatctcg
     4141 gccatcacag aagctccgcg tgatgggcac gcgcgacgaa caccgtgacg tcgtcgcggc
     4201 cacgcgtgaa gtcgcgatac agcgtagcgg cgaccagggt tgggtggcgc agcgtgagac
     4261 ccggatagcg gctcagatcc caacgcgtgg cgagaccgtc ggagtgcagg atcagcaaac
     4321 cgtcttgcgg ccagtcgacc tgaaattcct gcaccttgcg caattgatgg ccgacgatgc
     4381 cggcgtggga caccagatga cgatgaccgt acggcgtcca gacgctcgcg gcgatgttgc
     4441 ccacgccgca aaagcgcgca acgccaggcg cgccgttcgc gacggcctga gccggaatgc
     4501 gcgcgacgcc gagcgcggcg ccgcgggtgg gccgcagcgc ttcgtgtgag gccgcgacga
     4561 tttcctcgac gctcgaaggt ccgagccggg caaacgtagc ggtcgcggca acggccgcgg
     4621 tattcgccag tgcgccgtgg ccgagtccgt cggcgaccat taccgtgaat tcgccctcca
     4681 tgtagccgca cgtccatgcg tcgccgcata acgtctcgcc ctgcaacggc agattgatcg
     4741 caccgtattc gatcggcccg caaggctccg cgtccgccga gccgggctcg gcccagacca
     4801 cgatgcgtag caccgtgccc agacccgggg cgctccagat gtcgagctcg tcggcgaggc
     4861 gttgaatcgc gcccatgccg ttgccgggac tgccggcggt cgtatagccg tcgacgaaac
     4921 aggcgtgcag atcgtggata cccggaccgc tgtcgagcgc gagcagttcg atgccgtaac
     4981 gcgacggcag cgcctcactg atgggccgca ccagcaattc gccacgctgc gcgtgcttga
     5041 gctgattggt cgcgcattcg gtcacgatga tcgccagcct gccgatcacc gtttcactgc
     5101 agccggtgcg atgcgccaac tcactgatac cgcggcgcgc gtacgaaacc tggctcggat
     5161 cgccgatttc gaagcgctgt tgggtggcgc tcaacccgtt cagaacggct tccatttggt
     5221 caccgaaata tgagtaccct cgcctggcgc cgagcggatt tcgaactcgt cgcacagccg
     5281 tttcgcgccg ccgagcccga ggcccagacc attgccgctg gtatagccgt cggtcagcgc
     5341 tttttgaatg tcggcgatgc ccggtccggt gtcgacgaac tcaaggcgca agccggtacg
     5401 gccatcacgc tgaacgatat aaaagtgtac gtcgccgccc ccgccgtacg tgagcgtgtt
     5461 gcgggcgagt tcactcgccg cggtgacgaa tttcgtctga tcgattaacg acatgcctag
     5521 cgccacggcc ttttcgcgca cgaaacggcg taagcgcacg atctgctcgt cggagcggat
     5581 cggcatgatc tcctcgggag gctggccgtc aggaccagcc gggccactcg ggacggacgc
     5641 atcgtcgttg cccgtcatgg aggagagcgg cacgttatcg cgcggccggc cgggaatggc
     5701 gctcaaagcg gttccgtacc gctcagcaac gccatgccct tctcgacatt cagcgcggtg
     5761 cgtacgccag gcagcgccag accgagttcg accagcgtaa tcgcgacgga cggctgcatg
     5821 ccgaccacca cggtttgcgc gtcgagcacg cgcgccatcg ccgccgtgtc gccgatcatg
     5881 cggccaatga acgaatccac cacgtcgagc gatgaaatat cgatcagcac gcccttggcg
     5941 cggtctttga cgatgcggct cgtcagatca tcctgcaagg tcatcgccag acggtcgtgc
     6001 atgtctacct ggatagtgac aagcagcagc ttgcccatcc gcaaaattgg aatgcgttcc
     6061 atcgtggggt ccggttgggg ggcacgtgaa ccggcgtcag tcgccggcga acgggttgcc
     6121 gcgcaatgcg gccggcgaac cggagtggcc cggggcttcg gcatgacgat cgccgttcga
     6181 gagcgacctg ccggtgcgtt gcagcgcaat gacgaacgca tcggcaagcg tggccttggt
     6241 gatcacattc gacagattca cgcccagatg aacgatggtc tgcgcgattt gcggccgaat
     6301 cccgctgatg atgcaatcgg cgcccatcag ccgcgcggct gccacggtct tgagcagatg
     6361 ttgtgcgacg agggtatcga ccgtcggcac gcccgtgatg tcgatgatcg cgatcgcggc
     6421 gccggtctcg acgatcttct gcagcaggct ttccatcacc acctgagtgc gtgccgagtc
     6481 tagcgtgccg atcagcggca gcgcgagaat gccttcccac agttgcacga ccggcgtgga
     6541 taattccagc agctcctgct gctgacgcat gatgacctgc tcccggctcg tctggaaaac
     6601 ctccatggtg tagaggccga gcgcgtcgaa caagctgccg atggtccagc tcagttccgc
     6661 cagcgtggcg ggatcgttcg ccaactcgct gcgcaaacgt gcaaagagcg gttccttcag
     6721 cgagaacacg aacatggcgg tttcaaccgg tgtaaaaccc tgccgcgcgc gctgcgtcga
     6781 tacttccgcg agcaggccgc gcacgtcgtc ccatgccggt gttttgaact cgacgcggtc
     6841 ggacgcgcca agcgctgcgc aggtcaaccc gacgaactgc acgaactggt tgcgcaattg
     6901 cgcttcggtg acaccaccgc ggcgcgcggc gatcgcgtat tgctgagcga cccactcgcc
     6961 caataactcg ctttgccgtt cattgaatag ttgcgcgaga cgcgttccgc
//
LOCUS       DATASH010001048         9196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1369153,
            whole genome shotgun sequence.
ACCESSION   DATASH010001048 DATASH010000000
VERSION     DATASH010001048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 9196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9196
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1369153"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(567..1346)
                     /locus_tag="VHV99_27790"
     CDS             complement(567..1346)
                     /locus_tag="VHV99_27790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX3639216.1"
                     /translation="MSSRMRLATVVGDSPQIRIALALLAALIVWIVFLFQLGRWLSTT
                     TSAHAPARPLEMRVVELDPPKAQVATSSEPPVTHATSTPSTPFTPSAPEKPRQPAEPH
                     AHVLTQPRATPPVPSKPDAQSVQSIAPAHSAPPQEPKLAADPPARVAAPSSEQSTSTA
                     RSATSSGDTSGDTSAHAIVQPLPELPDDLRDQAYQTIATARFMIHADGSVDVELIKPT
                     PNPRLNQILLEALRKWRFFPAMQAGHPVESRQDVRVHFNVN"
     gene            complement(1339..1737)
                     /locus_tag="VHV99_27795"
     CDS             complement(1339..1737)
                     /locus_tag="VHV99_27795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX3639217.1"
                     /translation="MRFKRSRAAKRGRIEIIPMIDVMFFLLATFMLASLAMQRLDAVK
                     VNLPAGSAQKMAAHEPLTISVDSRNDVFVNHQAVRPDQVEPTVKRLLQPDGNVVIAAD
                     DAAGHGVVVQAMLAARRAGAERFLIAVRHE"
     gene            complement(1734..2381)
                     /locus_tag="VHV99_27800"
     CDS             complement(1734..2381)
                     /locus_tag="VHV99_27800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX3639218.1"
                     /translation="MQDWASVLAAVRVGGWVIYPLTILAILAVAIALDRSYVFLRFGR
                     LPAAMRGTHEIDGPGPSLDWAGRLKDVASQNAFTRMSAPWAPDPSVPLWLREAKAQAL
                     ASQIERDMSKGFWVLETIVTAAPLLGLLGTIVGMMHSFQLFGGDGLVDPGGVTGGVAQ
                     SLVATAIGLVVALFALFAFNYFSRRLERLMEDLESFANARLSETRLADTPAGALP"
     gene            complement(2409..4754)
                     /locus_tag="VHV99_27805"
     CDS             complement(2409..4754)
                     /locus_tag="VHV99_27805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX3639219.1"
                     /translation="MKNRTRVSHAALLLFAIAAHAHAAPNEGSVTGAVTDIAGKPVRN
                     ATVSVQNASGAAVNETKTDADGHFAIAHVTPGTYAIVVTAPGFASGNTIATTTAGTES
                     AVSVKLVQSDTLDVQVNAKRLDRARNGLLPETGSSVYRFSQADIDTLPAGQDTPLNQV
                     LLQAPGVASDSYGQLHVRGDHANLQYRINGIIIPEPISGFGQALDTRIIDQVNLLTGA
                     LPAQYGYRTAGVVDIRTKTGDAGNGGSIDVFGGSHQSIKTSADVFGNQGPFSYYFSGS
                     LGMNNLGIENPTASANAIHDHTRQGDAFGYMSYIINPLTRVSVMFGTTSNQFEIPNTP
                     GLPTNFTLNGSSTFDSSQLNETQSELNNFAAVALQGTNGGALDYQVALFTRYTRTQFN
                     PDPVGDLLFNGVASQDFHSNSANGAQADTTWRLNDKHTIRAGLFFQQEHAVFDDSVNV
                     FAADADGNQLSDQPFNIQDSSSKTGYLYSAYVQDEWKITDKLTLNYGLRYDKMDEYIS
                     ASQLSPRIGLVYALTPATTLHAGYARYFTPPAFELVSNSTISRFNGTTNQSPSNQNDP
                     VQPERSHYFDLGVTQRLGSNVTVGLDAYYKKSTNLLDEGQFGSALIFTPFNYREGRVY
                     GVEFTANYKQGNLSAYLNLAWSRAQGTQIDSAQFNFDPAELAYINSHWVYLDHDQRIT
                     ASFGGAYAFGRTTFTFDGTVGSGLRSGFANTDRLPLYTQVNLGVIQHFNQPLIGKFDV
                     RLLVVNAFNRVYELRDGSGIGVGAPQYGPHFAVYAGVTKNF"
     gene            complement(5118..5918)
                     /locus_tag="VHV99_27810"
     CDS             complement(5118..5918)
                     /locus_tag="VHV99_27810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3639220.1"
                     /translation="MKVLPLVASASALSLSACLFASTGAMAQDVQPRNVPVHNIVLVH
                     GAWVDGSGWQPVYDRLTKDGYHVTIVQEPLNSLEDDVAATKRVLDRQNGPTILVAHSY
                     GGSVITEAGVHPNVVGLVYVAAHAPAVGEDESTLGKGMPSYTSKQAGAIMKTGDGYTY
                     LNPADFPADFAADLPLKEARFESHAQILTAAKVFSTPMTVAAWTSKPSWGIVAADDKI
                     ISPDLERWYYARAHSHTTVLAGASHSVYESRPKEVAAVIEDAARHAQL"
     gene            complement(6030..6380)
                     /locus_tag="VHV99_27815"
     CDS             complement(6030..6380)
                     /locus_tag="VHV99_27815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4148 domain-containing protein"
                     /protein_id="HEX3639221.1"
                     /translation="MKNALVCFALTVGAMAAPALSFAQSNAPVTRAEVRADLVRVEQA
                     GYTPSSGDQANYPADIQAAEAKIAAHGEHQMVQQSYGGVAQNGSASSGTSRHMPTPSS
                     CVGPVSFCNIFFGN"
     gene            complement(6445..6795)
                     /locus_tag="VHV99_27820"
     CDS             complement(6445..6795)
                     /locus_tag="VHV99_27820"
                     /inference="COORDINATES: protein motif:HMM:NF025045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4148 domain-containing protein"
                     /protein_id="HEX3639222.1"
                     /translation="MKLKLAMTLSMILLASAGAVTQASAQEKTRTEVRHELIQAEANG
                     SRFVTDTSYPAINPIFSQQAARLKMSTSGEGPAMSGSTAAGHRVSSSPVHASSAAMTA
                     CVGPAGFCTPYFGS"
     gene            7425..>9196
                     /gene="acs"
                     /locus_tag="VHV99_27825"
     CDS             7425..>9196
                     /gene="acs"
                     /locus_tag="VHV99_27825"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEX3639223.1"
                     /translation="MSTSDSILEEPQAFLPHPGVAAYAAISGMDAYRALVAEAEGDYA
                     GFWARRAREMLDWKTPFSTVLDESRAPFYEWFNDGTLNASYNCLDRHVEAGNGEHVAV
                     IFEADDGSVTRVTFRQLLERVSRFANALRTRGVKKGDRVVIYMPMSIEGIVAMQACAR
                     IGATHSVVFGGFSSKSLNERIVNVGAIALVTCDEQMRGGKALPLKNIADEALAMGGCE
                     AVKSVIVYQRTGGKIAWHEGRDLWMHELTQAEPDTCEPEWVSAEHPLFILYTSGSTGE
                     PKGVQHSTGGFLMWAAQTMKWTFDAKRSDVFWCTADIGWITGHSYIAYGPTAIGATQV
                     VFEGVPTYPNAGRFWEMIAKHRVTVFYTAPTAIRSLIKLAESDPAVHPDRYDLSSLRL
                     LGTVGEPINPAAWTWFYEHVGHSHCPVIDTWWQTETGGHMIAPMPGATPLVPGSCTLP
                     LPGIMAAVVDETGQDVPNGQGGILVVKRPWPSMLRGVWGDPQRYRKSYFPEELGGKLY
                     LAGDGAVRDEDTGYFTITGRIDDVLNVSGHRLGTMEIESALVANPLVAEAAVVGRADE
                     TTGEVVVAFVVLKGERPTGDQAIR"
BASE COUNT         1700 a         2665 c         2960 g         1871 t
ORIGIN      
        1 tccggttatt ggccacgacg ttgtgaactg cgggcgatga cgcacgcgaa cgctgcgcgc
       61 ttgctacctg tttgcgatct gtttgcgatc cggtttggaa tctgatgcga ttccgaggca
      121 atccgaaacg aaccgcctaa atcacttgga ttgatttagt acagcaggcg ggtgggcgcc
      181 cgtcaaggaa atgatccggg aggacgtcga cgggccgagg cggtcgcgag ggcggcaggg
      241 catacggggc ggggcctggc gggcgtgatg cggtgcaaga tggcgtgtcg gagcttgggg
      301 aaagtcctgg ctcgtttgaa agtaaattga aaacttaagt gatctttcgc gacggtattt
      361 ggtgcgttcg cgaagatatt cagtggcggc tgagcgcggc gattctccca ctgacgatcg
      421 cggcgatgcc ccgcgccgag cggcgactca accaggccgt ttttccgcgg cttcattcaa
      481 cgccccttga ccttgctcgt ttgagcttcg agaggccgcg ctggtgatgc ggtcgttgtg
      541 agtctgatgg cactaccctc ggcgcgctag ttcacattga aatgcacacg gacgtcctgg
      601 cggctttcca cgggatggcc ggcttgcatc gcggggaaga agcgccattt gcggagcgct
      661 tcgagcagga tctgattgag ccgcgggttc ggggtcggtt taatgagttc gacgtcgact
      721 gagccgtcgg cgtgaatcat gaagcgggcc gttgcgatcg tttgataggc ctggtcgcgt
      781 aaatcgtcag ggagttccgg caacggttga acaatggcgt gcgcgcttgt atcgccggat
      841 gtatcgccgg acgatgtggc cgatcgcgcg gtgcttgtgc tttgttcgga agagggagcc
      901 gccacgcgcg ccggtgggtc ggccgccaat ttcggttcct gtggtggcgc cgagtgagcc
      961 ggtgcaatgc tttgcaccga ttgcgcatcc ggcttcgatg gcacaggtgg tgtcgcgcgt
     1021 ggctgtgtca agacgtgagc gtgcggttcg gcaggttgtc gcggcttttc cggcgcggac
     1081 ggagtgaacg gagtggacgg agtagacgtg gcgtgcgtaa ccggaggctc agaagaagtt
     1141 gcaacctgag ctttcggcgg atcgagttcc accacgcgca tctcgagtgg cctcgcaggt
     1201 gcgtgcgcag acgtggttgt gctcaaccag cgtcccaact gaaacaggaa cacgatccat
     1261 acgatcagcg cggccagtaa cgccagcgcg attcgtattt gcggtgaatc gcccacaact
     1321 gtcgcgaggc gcatccggct actcatggcg tactgcgatc aggaaccgct ctgcgcctgc
     1381 gcggcgcgcg gccagcatgg cctgaacgac cacgccgtgg cccgctgcgt cgtcggcggc
     1441 gatcaccaca ttgccgtcgg gttgcagcag gcgtttcacc gtcggctcga cctgatcggg
     1501 acgaacggcc tgatggttca cgaatacatc attgcggctg tccactgaaa tagtcagcgg
     1561 ttcgtgcgcc gccatctttt gcgcgctgcc ggcgggaagg ttgactttga ctgcgtcgag
     1621 ccgttgcatc gcgagcgatg cgagcatgaa ggttgccaat agaaagaaca tcacatcgat
     1681 catcggaatg atttcgatgc gaccgcgttt cgccgcgcgc gaacgcttga acctcacggt
     1741 agcgctccgg cgggcgtgtc ggcaagacgg gtttcgctga gccgcgcgtt agcaaacgac
     1801 tcgaggtcct ccatgagccg ttcgagccgg cgggagaaat agttgaacgc aaagagcgcg
     1861 aacaaggcga ccacgaggcc gatcgcggtt gccaccagcg actgtgccac gccgccggtc
     1921 acgccacccg gatcgacgag accgtcaccg ccgaacaact ggaacgaatg catcatgccg
     1981 acgatggtgc ccagcagacc cagcagaggc gccgcggtta cgatggtttc cagtacccag
     2041 aagcccttgc tcatgtcgcg ttcgatttgc gaggcaagcg cttgcgcctt tgcctcgcgt
     2101 agccacagcg gtacggaagg atcgggcgcc cacggcgcgc tcatccgcgt gaaggcgttc
     2161 tgactggcga catcttttaa cctgccggcc caatcaagcg acggcccggg cccatcgatc
     2221 tcgtgcgtcc cacgcatggc agcgggcaat cggccaaaac gcaggaatac gtacgatcga
     2281 tcgagggcga tggcgaccgc cagaatggcg agtatcgtca gcggatagat gacccatccg
     2341 cctacgcgta ccgccgccag aacacttgcc caatcttgca tgacctacct ccttgtagcc
     2401 tgaccgaatc agaaattttt ggtgacgccg gcatagacgg cgaaatgtgg cccgtattgc
     2461 ggcgcaccga cgccgatacc cgatccgtca cgcaactcat agacgcgatt gaatgcattg
     2521 accacgagca ggcgtacgtc gaatttgccg atgagcggct ggttgaagtg ctgtatcaca
     2581 ccgagattga cttgcgtata gagcggcagc cgatccgtgt tcgcaaagcc gctacgcagg
     2641 ccgctgccca ccgttccatc gaaagtgaag gtcgttctgc cgaacgcgta ggcgccgccg
     2701 aatgacgcgg tgatgcgctg gtcgtgatcc agatagaccc agtgactgtt gatatacgcc
     2761 agttccgccg ggtcgaaatt gaactgcgcc gaatcgatct gggttccctg cgcgcggctc
     2821 caggcaagat tcaggtacgc ggacaggttg ccctgcttgt agttggccgt aaactcgacc
     2881 ccgtacacgc gcccctcccg gtagttgaac ggcgtgaaga tgagcgcgga gccgaactgt
     2941 ccttcatcga gcaggttcgt ggacttcttg tagtaagcgt cgagtccgac cgtcacgttc
     3001 gacccgagac gctgggtcac accgagatcg aaatagtggc tgcgttccgg ctgcaccggg
     3061 tcgttctggt tcgacgggct ctggttggtc gtgccattga agcggctgat ggtcgagttg
     3121 gacaccagct cgaatgcagg gggcgtgaaa tagcgcgcgt agccggcatg gagcgtcgtg
     3181 gccggcgtca gcgcatagac aaggccgatg cgagggctga gttggctcgc gctgatgtat
     3241 tcgtccatct tgtcgtagcg aagaccatag ttcagcgtga gcttgtccgt gattttccat
     3301 tcgtcctgca cgtaggcgct atacagataa ccggtcttgc tgctcgagtc ctgaatattg
     3361 aagggctgat cggacagctg gttgccatcg gcgtccgccg cgaatacgtt gacgctgtcg
     3421 tcgaacaccg cgtgttcctg ctggaaaaag aggccggctc gaatcgtatg cttgtcgttc
     3481 agccgccagg tcgtgtccgc ttgcgcgcca ttggccgagt tgctgtgaaa gtcctgcgac
     3541 gccacgccgt taaagagcag gtcgccaacc gggtccggat tgaattgcgt acgcgtgtag
     3601 cgggtaaaca acgcaacctg atagtccagc gcgccgccat tggtgccctg gagggctacc
     3661 gcggcgaagt tgttcaactc cgattgcgtt tcgttcaact ggctcgaatc gaacgtgctg
     3721 ctgccgttaa gcgtgaagtt ggtcggcaag cccggcgtgt tgggaatctc gaactggttg
     3781 ctggtcgtgc cgaacattac gctcacgcgc gtgagcgggt tgatgatgta agacatgtag
     3841 ccgaacgcat cgccctggcg cgtgtggtcg tgaatcgcgt tggcactcgc ggtcggattc
     3901 tcgatcccga gattgttcat gccgagcgag ccgctgaagt agtaactgaa tggcccctga
     3961 ttgccgaata catccgcgct ggtcttgatg gattgatggc tgccgccgaa cacgtcgatg
     4021 gacccaccgt tgcccgcatc acccgtcttc gtgcgaatgt ccaccacgcc tgctgtacgg
     4081 tagccgtact gcgccggcaa cgcgccggtc agcaggttca cctggtcgat gatgcgggtg
     4141 tcgagtgcct ggccgaaacc gctgatcggc tctggaatga tgatgccgtt gatgcggtat
     4201 tgcagattcg cgtggtctcc gcgcacgtgc aactggccgt atgagtcgct tgccacgccg
     4261 ggcgcctgga gcagcacctg gtttagcggc gtgtcttgcc ctgcgggcag cgtatcgatg
     4321 tcggcctggc tgaagcggta cacgctgctg ccggtctccg gcaacaatcc gttgcgggct
     4381 cgatcgagcc gctttgcatt cacctgaacg tcgagtgtgt cgctttgaac gagtttgacg
     4441 ctcaccgcgc tttccgtgcc cgccgtcgtg gtcgcaatcg tgttgccgct cgcgaagccg
     4501 ggggcggtaa cgacgatcgc ataagtgccc ggtgtcacgt gagcgatcgc gaagtggcca
     4561 tcggcgtccg tctttgtctc attgactgcg gcgccgcttg cgttctgaac gctcacggtg
     4621 gcgtttcgga cgggcttgcc cgctatgtcg gtgacggcgc ccgtcaccga gccttcgttg
     4681 ggcgcggcat gggcgtgggc ggcgatagcg aacagcagca aggctgcatg ggagacgcgc
     4741 gtgcggtttt tcatcgttat ttattcaggg ttgaccacga ggtggctgcg ttgccgtctt
     4801 gtcgacgttt ttcttgcgtg cgacacggat gacgggcgtg cgcttaccgg cccccgatct
     4861 agcgggatcc tcgtcatcgt tcgtcgcaaa gaatgttata tcattacatt tcaaaactaa
     4921 aatgacaact ggatggacgg tgctgcgagt gatctgggga ggtgaggaga gcgtttgacg
     4981 cgtaagtgga agcccggatt cgggccgccg gaatcagtcg gcacggtgat cgtgcctgga
     5041 tccgatgacg gcccaaggcg gcccgtcatc gaatgcacaa ccgggttgct ccgcgagcgt
     5101 actgctcatc ggtccgctca caactgagcg tgcctggccg cgtcttcgat cactgcggcg
     5161 acttctttcg gacgcgattc atagaccgaa tgactcgcgc cggcgagcac cgtcgtgtga
     5221 ctatgcgcac gggcgtagta ccagcgttcg aggtcggggc tgatgatttt gtcgtcggcc
     5281 gcgacgatgc cccagctcgg cttgctggtc catgcggcca ccgtcatcgg cgtcgagaag
     5341 accttcgccg cggtcaggat ttgcgcgtgc gattcgaacc gggcttcttt gagcggcaga
     5401 tcggcggcga aatccgccgg gaaatccgcg gggttcaggt acgtgtagcc gtcgccggtc
     5461 ttcatgatgg cgcctgcctg cttcgaggtg tagctcggca tgcctttacc aagggtgctt
     5521 tcatcttcgc cgacggctgg cgcatgcgca gccacgtaca ccagaccgac cacgttcgga
     5581 tgcacgccgg cttcagtgat gacggagcca ccgtaactat gggccacgag gatcgtcggg
     5641 ccattctgcc ggtcaaggac gcgtttggtt gcggcgacgt cgtcttcgag tgagttcagc
     5701 ggctcctgca cgatggtgac gtggtagccg tctttcgtca gtctgtcgta gaccggctgc
     5761 cagcccgatc cgtccaccca tgcgccgtgc acgagaacga tattgtggac cgggacattc
     5821 cggggctgga catcttgtgc catcgctccg gttgacgcga aaagacaagc cgacaatgac
     5881 aaggccgagg ccgaggcgac gagaggcaag actttcatga caccctccgt ttgtaattaa
     5941 aaaacgttgc aatagttctc ttgggttaag tggaaatagt agtagtactg tgcatattga
     6001 agacggcacg gaacttcatt ccgcaccgat cagttaccga agaagatatt gcagaagctg
     6061 accggaccca cgcaggaaga gggcgtcggc atgtgcctgg acgtgcccga cgaagcgctg
     6121 ccattctgcg caacgccacc ataggattgc tgcaccatct ggtgctcgcc gtgcgcggcg
     6181 atctttgctt cggcggcctg aatgtcggcc ggatagttgg cctggtcgcc gctcgacggg
     6241 gtgtagcccg cctgttcgac gcgcaccaga tcggcgcgga cttcggcgcg tgtcaccggc
     6301 gcgttcgatt gagcgaagct cagagcggga gcggccattg cgccgacggt gagagcgaaa
     6361 caaacaagag cgtttttcat gattggtact ccttttacaa gtggacgaat tgcgggaaaa
     6421 cgattgcggt aaaaaagcga ttcattagct gccgaagtac ggtgtgcaga atcctgcagg
     6481 gccgacacat gctgtcatcg ccgcgctgga tgcgtggacg ggcgaggagg aaacgcggtg
     6541 gcccgccgcc gtcgaaccgg acatcgccgg accctcgcca ctcgtgctca tcttcaggcg
     6601 cgcggcctgt tgcgagaaga tcgggttgat cgcggggtac gacgtgtcag tcacgaagcg
     6661 tgaaccgtta gcttcagcct gaatcagttc atggcgcact tccgtgcggg ttttttcctg
     6721 cgccgatgcc tgggtgactg cgccggccga tgcaaggaga atcatcgaaa gagtcattgc
     6781 aagtttgagt ttcattgtgg atacctaatt gatggtgatt gaatctaact aactatgtga
     6841 ttgggaaact acgtcgttac tcgtccatct atgtggtcca ggtgctgctg gcgttgctcg
     6901 gaagcgctgc tgctttcgct cgtggaccgt cttcttgttg ccgtctatgc atgggcaaac
     6961 acagcgaggt gccgcttatt cccatttttt ttcagcgccg gaaaagggcg cggactttgc
     7021 cgaatatttc ggcatacaaa cggctgacaa ccggtcactc cggattgccg cgcaacgtca
     7081 gtgtgagttt tcctgcttcg tgccgctggg gcgctttcta tcggctcccg tttgcgccgg
     7141 ccgggcctgt acgccaccgt accgacttgc tcccctcacg cgagcaccgt cattacaagc
     7201 ctttcgggtt tctccgtcac cgcgatcgaa acagctcgtg tgacaaggct tccctgaccg
     7261 atttgtgcgc catccccatt ccgcccagag cggcaaacgc ggggatcgcc ccgatcatgc
     7321 ggatcgcctt gctgcgcccg ctcgacaccc cgcgcgcggc aagggggcgt gactcgccgc
     7381 agcgcactca tcaagcaagc gcgaaagcgc gcaagggaca caacatgtca accagtgatt
     7441 cgattctgga ggagccgcag gccttccttc cccaccccgg tgtggcggct tacgccgcga
     7501 tctccggcat ggacgcctat cgcgcgctgg tcgccgaggc cgaaggcgat tacgcaggat
     7561 tctgggcgcg ccgcgcccgc gaaatgctcg actggaaaac gccgttctcc acggtgctgg
     7621 acgaatcgcg cgcgccgttc tacgaatggt tcaacgacgg caccctcaac gcgtcgtaca
     7681 actgcctcga tcggcacgtc gaagccggca acggtgagca cgttgcggtg attttcgaag
     7741 ccgatgacgg cagcgtcacg cgtgtcacct tccggcagtt gctcgaacgg gtgagccgct
     7801 ttgccaatgc cttgcgcacc cgcggcgtga agaaaggcga ccgcgtcgtg atctatatgc
     7861 cgatgtcgat tgaaggcatc gtggccatgc aggcgtgcgc gcgtatcggc gcgacgcatt
     7921 cggtcgtatt cggcggcttt tcatcgaagt cgctcaacga gcgcatcgtc aacgtgggtg
     7981 cgatcgcgtt ggtcacctgc gacgaacaga tgcgcggcgg caaagcgctg ccgctaaaga
     8041 acatcgcgga cgaagccctc gcgatgggcg gctgcgaagc ggtgaagagc gtcatcgttt
     8101 atcagcgcac gggcggcaag attgcgtggc acgaaggccg cgacctgtgg atgcacgagc
     8161 tgacgcaagc cgaaccggac acgtgcgaac ctgaatgggt gagcgcggaa catccgctct
     8221 tcattctcta tacatccggc tcgacgggcg agccgaaagg cgtgcagcac agcacgggcg
     8281 gtttcctgat gtgggccgct cagaccatga agtggacctt cgacgcgaag cgcagcgacg
     8341 tgttctggtg taccgcagac atcggctgga ttaccggtca cagctatatt gcgtacggac
     8401 cgaccgcgat cggcgcgacc caggtcgtgt tcgaaggcgt gcccacttat ccgaacgccg
     8461 gccgcttctg ggaaatgatc gcgaaacatc gggtgacggt gttctacacc gcgccgaccg
     8521 cgatccgttc gctgatcaag ctggccgagt ccgatcccgc agttcatccg gaccgatacg
     8581 acctgtcgag cttgcgtctg ctcggcacgg tcggcgaacc gatcaacccg gccgcgtgga
     8641 cgtggtttta tgaacacgtc ggccatagcc actgtcctgt catcgacacc tggtggcaaa
     8701 ccgaaacggg cggccacatg atcgcgccga tgccgggcgc gacgccactg gtaccgggtt
     8761 cgtgcacgtt gccgctgccg ggcatcatgg ccgcggtagt cgacgaaacc gggcaggacg
     8821 tgccgaacgg gcagggcggc attctcgtcg tcaaacggcc gtggccgtcg atgctgcgcg
     8881 gcgtctgggg cgatccgcag cgctacagga agagctattt ccctgaggaa ctcggcggca
     8941 agctgtacct cgccggtgac ggcgcggtgc gtgacgagga caccggctac ttcacgatca
     9001 ccggccgtat cgacgatgtg ctcaacgtat cgggtcaccg gctcggcacg atggagatcg
     9061 agtcggcact cgtcgcgaat ccactcgtcg ctgaggcggc ggtggtcggc cgtgccgacg
     9121 agacgaccgg cgaagtcgtg gtcgcgttcg tcgtgttgaa aggcgagcgg ccaaccggcg
     9181 accaggcgat caggat
//
LOCUS       DATASH010001049         3360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_7104222,
            whole genome shotgun sequence.
ACCESSION   DATASH010001049 DATASH010000000
VERSION     DATASH010001049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7104222"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="VHV99_27830"
     CDS             complement(<1..801)
                     /locus_tag="VHV99_27830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEX3639224.1"
                     /translation="MNPLSHALSRNGNNFDLVRLLAAVAVVYGHSYLLQAPDGTTDWV
                     QNALGFDGFGALGVYAFFLMSGMLVTASFDRQRSVPRFVALRIARLWPAVAGGSLVTV
                     FILGPLFTTLPLRDYFASGATWGNLDNFSTIVLKTGWALPGVFEHNRFAVDVCGPLWT
                     LPLEVRCYLIVMVTGMLGLLSSARGVALAVVLGCAAFVFRVHLPHLQIGLRDFSETPG
                     GYSFWPEPFFLLGMLLYGWRERVDINGLAALALAMVFLVFRDTAGAQPL"
     gene            1254..>3360
                     /locus_tag="VHV99_27835"
     CDS             1254..>3360
                     /locus_tag="VHV99_27835"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX3639225.1"
                     /translation="MSTTPSTHDNPLLDFSDLPRFGDIRPEHVTPALDILLADAAAAV
                     ERAAQPITPASWADVVEPVERATEPLSRAWSVVGHLNAVADTPELRAVYGENLPRVTE
                     FWSSVGQNLALYEKYKALTASSDFASLTGERKKILGNALRDFRLSGAELPEDQKPHFA
                     ELQERQAGLSKAFSDHVLDATNAFAYLVEAGAEAQLAGLPEDVIEGAKEAAEREGKTG
                     YKFTLHFPSYFPVMQYSENRAMREAMYRAYVTRASELGPQYGKGKPEWDNTQVLADQL
                     KLRAEEARMLGYNNFAEVSLAPKMAESPAQVMAFLEDLATRARPHAEQDWKELREFAA
                     NELGMSELQPWDMTFAAERLRQKRYSFSENEVKQYFPEDAVFKGLFKVTETLFGVRIR
                     RDEAAVWHPDVRFFRVENQDGGLVAQFYLDLYAREGKRGGAWMDDARGRHKHTHGSVQ
                     TPVAYLTCNFSAPVGGKPACFTHDEVITLFHEFGHGLHHMLTRVDELGVSGINGVEWD
                     AVELPSQFMENFCWEWDVLSDMTSHVETARPLPRDLFDKMLAAKNFQSGLGTLRQIVF
                     SMFDMQLHTGFDASGTKNATELASEINERFHVVPQASFSRWPNTFSHIFAGGYAAGYY
                     SYKWAEVLSADAYAAFEEAAQAASGSVLDQATGMRYRKEILEVGGSRPAMESFKAFRG
                     REPNIDALLRHNGMTPGAAH"
BASE COUNT          706 a         1107 c         1022 g          525 t
ORIGIN      
        1 cagcggctga gcgccggccg tgtcgcggaa caccagaaat accatcgcca gcgcaagcgc
       61 cgccaagccg ttgatgtcga cccgctcgcg ccatccgtac agcagcatcc ccagcaagaa
      121 aaatggctcc ggccagaacg aataaccgcc gggcgtttcg ctgaagtcac gcagcccgat
      181 ctgcagatgc ggcagatgca cgcggaaaac gaacgcagcg catccgagca cgaccgccag
      241 cgccacgccg cgcgcgctgg acagcagccc aagcatgccg gtcaccatca cgatcagata
      301 acagcgcact tcgagcggca aggtccacag cggcccgcag acgtcgacgg caaagcggtt
      361 gtgctcgaat acgccaggca gcgcccagcc agttttcagc acaatggtgg aaaagttgtc
      421 gaggttgccc cacgtcgcgc ccgaggcgaa atagtcgcgc agcggcagcg tcgtaaacag
      481 cggcccgagg atgaacacgg tcaccagcga accgcccgcc actgccggcc acaagcgcgc
      541 aatgcgcaac gcgacgaaac gcggcaccga gcgttgccgg tcgaaactcg cggtcaccag
      601 catgccgctc atcagaaaaa acgcatagac gccgagcgcg ccgaaaccgt cgaaaccgag
      661 cgcgttctgg acccaatccg tcgtgccgtc gggtgcttgc agcagatacg aatggccata
      721 caccacggcg acggcggcaa gaagccgcac cagatcgaag ttgttgccgt tgcgcgatag
      781 agcgtgcgaa agcgggttca tcgacacctc ttacagaaaa cgtcgccgac gggtagcgat
      841 acgccagaat gcggcacagc agcaattcgc accgttcgct ttcgcacaga gcagcgcggc
      901 atagcgggag cggcatgcgg gagcggcccg cgacgcatag gccaccgccc gcatgacacc
      961 tgaaagcgca cgcgactcct accgcccgaa tgccgcccga agcccgtgcg gcattcccac
     1021 cacccgaccg ccgcctgaag ggcacagcat ttcccccatc cgactacggc ccgaagcgcg
     1081 tgcgccattc gctcccgcgc gcgcagtcgc gcgacatctg ataatttgtc tgccgacgga
     1141 ttggaatcgg cgcgccctgc cccaataatg cctatatgag gtgttatcgc aggcgtcgcg
     1201 cgcgcccgtc gatgcaccga ttacccgttc acccgcgtca gacaggaagc cttatgtcca
     1261 ctaccccctc gactcacgac aatccgctcc tcgatttttc cgacctgccg cgctttggcg
     1321 acatccgccc cgaacacgtc acgcccgccc tcgacatcct gctcgccgat gcggccgccg
     1381 ccgtcgagcg cgccgcccag cccatcacac ccgcttcgtg ggccgatgtg gtcgaaccgg
     1441 tcgagcgcgc caccgaaccg ctgtcgcgcg cctggagcgt ggtcggccat ctgaacgccg
     1501 tggccgatac gccggaactg cgggccgtgt acggcgagaa tctgccgcgc gtcaccgaat
     1561 tctggtcgag cgtgggtcag aacctcgcgc tgtacgaaaa gtacaaggca ctgaccgcca
     1621 gcagcgattt cgcttcgctc acgggcgagc gcaagaagat ccttggcaat gcgctgcgcg
     1681 atttccgtct gtcgggcgcg gaattgccgg aagaccagaa gccgcatttc gccgaattgc
     1741 aggaacgcca ggcgggcttg tcgaaagcat tttcagacca cgtgctcgat gcgacgaacg
     1801 cattcgcgta tctcgtcgaa gccggcgctg aagcacaact ggccggcctg cccgaagacg
     1861 tgatcgaagg cgcgaaagaa gcggccgaac gcgaaggcaa gaccggctac aaattcacgc
     1921 tgcacttccc gtcgtatttc ccggtgatgc agtactcgga aaatcgcgca atgcgcgaag
     1981 ccatgtaccg cgcgtatgta acgcgtgcct ccgagctcgg tccgcaatac ggcaagggca
     2041 agccggaatg ggacaacact caggtgctcg ccgatcagtt gaagctgcgc gcggaagaag
     2101 cgcgcatgct cggctacaac aacttcgccg aggtctcgct cgcgccgaaa atggcggagt
     2161 cgccggcgca ggtgatggcc ttcctcgaag acctcgccac gcgcgcgcgt ccgcacgctg
     2221 aacaggactg gaaggaactg cgcgaattcg ccgcgaacga actcggcatg agtgagctgc
     2281 aaccgtggga catgacgttc gccgctgagc gtttgcgcca gaagcgttat tcgttctcgg
     2341 agaacgaagt caaacagtac ttcccggaag acgcggtatt caagggcctc ttcaaggtca
     2401 ccgagacgct gttcggcgtg cgcatccgtc gcgacgaagc ggccgtgtgg catccggacg
     2461 tgcgcttttt ccgcgtcgag aatcaggacg gcggcctcgt cgcccagttc tatctcgacc
     2521 tgtatgcacg cgaaggcaaa cgcggcggcg catggatgga cgacgcgcgt ggccgtcaca
     2581 agcacacgca cggcagcgtg caaacgccgg tcgcatatct gacctgcaac ttctcggcgc
     2641 cggtgggcgg caaacccgcc tgcttcacgc acgatgaagt gatcacgctg ttccacgagt
     2701 tcggccacgg gctgcatcac atgctcacgc gtgtcgacga actgggcgtg tcgggtatca
     2761 acggcgtcga gtgggacgcg gtcgaactgc cgtcgcaatt catggaaaac ttctgctggg
     2821 agtgggacgt gctgagcgac atgacttcgc acgtcgaaac cgccaggccg ctgccgcgcg
     2881 atctgttcga caagatgctc gccgcaaaga acttccagag cggtcttggc acattgcgcc
     2941 agatcgtgtt ctcgatgttc gacatgcaac tgcacaccgg tttcgacgcg tccggcacga
     3001 aaaacgcgac cgaactcgcg agcgagatca acgagcgttt ccacgtggtg ccgcaagctt
     3061 cgttctcgcg ttggccgaat acgttcagcc acatcttcgc gggcggctac gcggcgggtt
     3121 actacagcta caagtgggca gaagtgctgt ccgccgacgc ctacgccgcg ttcgaagaag
     3181 ccgcgcaagc cgcgagtggc agcgtgctcg atcaggcaac cggcatgcgg tatcgcaagg
     3241 agattctgga agtgggcggc agccgtcccg cgatggaatc gttcaaggcg ttccgtggcc
     3301 gcgagccgaa tatcgacgcc ttgttgcggc acaacggcat gacgcctggc gcggcgcatt
//
LOCUS       DATASH010001050         3178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4927249,
            whole genome shotgun sequence.
ACCESSION   DATASH010001050 DATASH010000000
VERSION     DATASH010001050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4927249"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="VHV99_27840"
     CDS             <1..587
                     /locus_tag="VHV99_27840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX3639226.1"
                     /translation="MVGVFDTVRKIQGYFDAGRTGRDWNLATAMVINGKAGMQFMGDW
                     AKGEFANAGKKSGSDYICAAVPGTEKSYTFNVDSFVFFQQKGQKAATPGQLALAKTIM
                     TPDFQEQFSLNKGSIPVRLGVSMAKFDDCAKKSYADEQVAIKSGGYVPSLAHGMAQPD
                     AAAGAISDVVTKFMNSQQDSKSAVAALAKAAKTK"
     gene            782..1723
                     /locus_tag="VHV99_27845"
     CDS             782..1723
                     /locus_tag="VHV99_27845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006025593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX3639227.1"
                     /translation="MTASISGNGKTTASVTRRTSPMAALADRWIPKLVLAPSVVISLV
                     FVYGFIAITGFLSLSNSRLMPRYEFVGLDRYRELFDNDVFWTSAANLGWFGIPFIGIC
                     IGLGLFLAILLDQQIRNEGALRAVFLYPMALSFIVTGTAWQWIMTPSVGLEKVFHDWG
                     WTSFSFSWLGDPDKAIFCVVIAAVWQSTGFVMALFLAGLRGVDGEIFKAAQMDGAGLP
                     TIYRKIVIPSMRPVFFSVLLILCHITIKTFDLVVALTAGGPGTSSSLPAIFMYTFSFN
                     RGQLGVGAASSMMMLATVVAVLVPLMYLESRSTRNAA"
     gene            1713..2570
                     /locus_tag="VHV99_27850"
     CDS             1713..2570
                     /locus_tag="VHV99_27850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006764932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX3639228.1"
                     /translation="MQPKMTISRAVIYAALILFALYFLFPLYVMLSTSFKDIDQLRTG
                     NLLTPPTHWTIDPWIKAWSGACTGVRCDGMQPFFMNSVRMVIPAVLISSIIGAFNGYV
                     LTHWRFRGADPIFTMILVGCFIPFQAILLPMARFEGFLGLSNTTTGLVVVHVIYGIAF
                     TTMFFRNFYVSIPAELVKAARIDGAGFFTIFTKILMPVSLPIFMVCLIWQFTQIWNDF
                     LFGIVFSGVDSMPITVALNNLVNTSTGVKEYNVDMAGAIIAALPTLLVYIIAGRYFVR
                     GLTAGAVKG"
     gene            2801..>3178
                     /locus_tag="VHV99_27855"
     CDS             2801..>3178
                     /locus_tag="VHV99_27855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3639229.1"
                     /translation="MASLSIRDVYKTYPNGVPVLKGVNIDIEDGQFLILVGGSGCGKS
                     TLLNMIAGLETVTKGEIQIDGKTVNNLSPKDRDIAMVFQSYALYPSMTVRENISFGLG
                     IRKVPKQEQAQIVDRVSNTLQITH"
BASE COUNT          572 a          943 c          970 g          693 t
ORIGIN      
        1 agatggtcgg cgtgttcgac acggtccgca agatccaggg ttacttcgac gcgggccgca
       61 cgggccgtga ctggaacctg gcaacggcca tggtcatcaa cggcaaggcc ggcatgcagt
      121 tcatgggcga ctgggcgaag ggcgaattcg ccaacgccgg caagaagtcg ggctcggact
      181 acatctgcgc tgccgtgccg ggcacggaaa agtcgtacac gttcaacgtc gactcgttcg
      241 tgttcttcca gcagaaaggc cagaaggctg caacgccggg tcaattggca ctcgccaaga
      301 cgatcatgac gccggacttc caggaacagt tcagcctgaa caagggctcg atcccggttc
      361 gtctgggcgt gtcgatggcc aagttcgacg actgcgcgaa gaagtcgtac gcggatgaac
      421 aggtggcgat caagtcgggc ggctacgtgc cttcgctggc acacggcatg gcgcaaccgg
      481 atgcagcagc cggtgcgatc tcggacgtgg tcacgaagtt catgaactcg cagcaggatt
      541 cgaagagcgc agtcgcagcg ctcgcgaagg cagcgaagac caagtaagtg atgtaagcga
      601 agtggcgcgc ctggctgttt cctcgtgggc gccgggtgcg cgcttctgat tgattgcagc
      661 aggtgcgtta cttagggtga gcgttccgcc ctggtttcat cgagtcacac ctatgccgtt
      721 ccggcctcgc ggtttccatg cgtgagcgcc ccggcggtac agtttcagta ggagtcgagt
      781 agtgactgct tctatcagcg gaaacgggaa aaccacggcc tccgtgaccc gccgcacgtc
      841 gccgatggcg gcccttgccg atcgttggat tccgaagctg gtgctcgcac ccagcgtcgt
      901 gatcagcctt gtcttcgtgt acggcttcat agccattacc ggctttctgt cgctgtccaa
      961 ttcgcgactg atgccacgtt atgaatttgt cggtctcgat cgttatcgcg agctgttcga
     1021 caacgatgtg ttctggacct cggctgccaa tctcggctgg ttcggcatcc cgttcatcgg
     1081 tatctgcatc ggcctcggcc tgttccttgc gattctgctc gatcagcaga tccgcaatga
     1141 aggcgcgctg cgcgccgtgt tcctgtaccc gatggcgctc tcgttcatcg tgacgggtac
     1201 ggcgtggcaa tggatcatga cgccgagcgt cggtctcgag aaggtgttcc acgactgggg
     1261 ctggacgagc ttctcgttca gctggctcgg cgaccccgac aaggcgattt tctgcgtcgt
     1321 gatcgcggcg gtgtggcaat ccaccggttt cgtgatggcg ctgtttctcg cgggcctgcg
     1381 cggtgtcgac ggtgaaatct tcaaggccgc gcagatggac ggcgcaggct tgcccaccat
     1441 ttatcgcaag atcgtgattc cgagcatgcg cccggtgttc ttctccgtgc tgctgattct
     1501 ttgccacatc acgatcaaga ccttcgacct ggtcgtcgcg ttgaccgcgg gcggtccggg
     1561 tacgtcttcg tcactgccgg ctattttcat gtacacgttt tcgttcaatc gcgggcagct
     1621 gggcgtcggc gcggcatcgt cgatgatgat gctcgccacc gtcgtggccg tgctcgtgcc
     1681 gctgatgtat ctggaatcga ggagcacccg caatgcagcc taagatgacg atcagccgtg
     1741 ccgtcattta tgcggccttg attctgttcg ccctgtattt cctgtttccg ctgtacgtga
     1801 tgctgtccac gtcgttcaag gacatcgatc aactgcgcac cggcaacctg ctcacaccgc
     1861 ccactcactg gaccatcgat ccgtggatca aggcatggag cggcgcgtgt acgggtgtgc
     1921 gttgcgacgg catgcagccg ttcttcatga actcggtgcg catggtgatt cccgccgtgc
     1981 tgatttcgtc gatcatcggc gcgttcaacg gttatgtgct cacgcactgg cgtttccgtg
     2041 gcgcggatcc gatcttcacg atgatcctgg tcggctgttt tattccgttc caggcgatcc
     2101 tgctgccgat ggcgcgtttc gaaggctttc tcggcctgtc gaacacgacc accggtctgg
     2161 tggtggtgca cgtgatctac ggtatcgcct tcaccacgat gttcttccgc aacttctacg
     2221 tgagcattcc ggctgaactt gtgaaggccg cgcggatcga cggcgccggt ttcttcacga
     2281 tcttcacgaa gattctgatg cccgtttcgc tgccgatttt catggtatgc ctgatctggc
     2341 aattcacgca gatctggaat gacttcctgt tcgggattgt gttctccggt gtcgattcga
     2401 tgccgatcac ggtggcgctg aacaacctcg tgaacacctc gaccggcgtg aaggaataca
     2461 acgtggacat ggccggcgcg atcatcgccg ccctgccgac cttgctcgtc tacatcatcg
     2521 ccggacgcta tttcgtgcgc ggcctgacgg cgggcgcagt gaagggctaa acccgggggc
     2581 aaacccgcgg gcaaacccgg actcaaccat acgcatcgcg gcggcgcttc tttgaacgac
     2641 acgcaccagg tgcaagaaac gccgccgtca tccgaccaga cgttcctgag aaaacgccgg
     2701 gccgccccaa gttttctcgg cccccctcgg gggggcctgg cgcgaagtgc caggtttggg
     2761 ggcgctttca agcagtacca gagacaagag gattcacagc atggcaagcc tttccatccg
     2821 tgacgtgtac aagacctacc cgaacggggt gccggttctg aagggtgtca acatcgacat
     2881 cgaagacggc cagttcctga ttctcgtcgg cggctcgggc tgcggtaagt cgacgctgct
     2941 caacatgatc gccggcctcg aaaccgtgac caagggcgag atccagatcg acggcaagac
     3001 ggtgaacaac ctctcgccga aagatcgcga catcgcgatg gtgttccagt cgtatgcgct
     3061 gtatccgtcc atgacggtgc gcgagaacat ctcgttcggc ctgggtatcc gcaaggtgcc
     3121 gaagcaggag caggcgcaga tcgtcgaccg cgtgtcgaac acgctgcaga tcacgcac
//
LOCUS       DATASH010001051         5302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3146642,
            whole genome shotgun sequence.
ACCESSION   DATASH010001051 DATASH010000000
VERSION     DATASH010001051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5302
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3146642"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VHV99_27860"
     CDS             complement(<1..280)
                     /locus_tag="VHV99_27860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015001481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEX3639230.1"
                     /translation="MSSPPATRRSDRTPPAGPRAGLRVWRWLAVLLVVAVLHWIAAQW
                     VERNRATLNPSNDEHVPVQVALLKPERIERNAAAGAGAPAPQPKRKPEA"
     gene            complement(498..1385)
                     /locus_tag="VHV99_27865"
     CDS             complement(498..1385)
                     /locus_tag="VHV99_27865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015001482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX3639231.1"
                     /translation="MPPIARSGAIRTDTGSTEPLEAPESEDESVETGEEKLRSGIQSI
                     EVGFRLLEVLTHEPRAMMLRDLAQRAGMSPAKAHRYLVSFLRLGVVAQDPLSGRYELG
                     GFALQLGLARLARVDGVKLARIALAELRDRLDLTVGIAVWGNQGPTMVHWMESSHPAK
                     ASLKLGDVMPLLSSATGLLFAAYLPSSKTAAMLERELADSRRSSQMGGPRTRDEVERV
                     LADVRQHEAARVEGMLLPTIHAFCMPVFDSTGDLALGLIALGHEGAFDIRWGGEIDTA
                     LRECAQKLSYELGYSAAPR"
     gene            1514..2497
                     /locus_tag="VHV99_27870"
     CDS             1514..2497
                     /locus_tag="VHV99_27870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX3639232.1"
                     /translation="MKLATLKDGTRDGQLIVVSRDLHTAAVADAIAPTMQRVLDDWAF
                     YAPQLQDLYDALNQGRARNTFPFDARECMAPLPRAFQWADGSSYVNHVELVRRARGAE
                     MPPEFWTDPLMYQGGSDDFIGPKDDVLCASEEFGIDFEAEVAVITTDVPMGATPDQAL
                     RSVRLVTLVNDVSLRNLIPAELAKGFGFFQSKPATSFAPVAVTPDELGESWREGRVHR
                     PMIVHWNNRKVGQPDAGTDMVFHFGQLISHAAKTRNLRAGAIVGSGTVSNKDAKRGYC
                     CIAEKRCLETIEHGAPQTEFMKFGDTVRIEMFDEAGKSIFGSIDQGIAPLD"
     gene            2845..4065
                     /locus_tag="VHV99_27875"
     CDS             2845..4065
                     /locus_tag="VHV99_27875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3639233.1"
                     /translation="MQRFESLERKTQRARASNVRATTSRRGLARLAAALCATLPAIAL
                     AQVKIGLVLSLTGPAASLGIPARDTATLLPKQIGGQSVEYIVLDDASDTTQAVQDTKK
                     LISENHVDAIIGSSITPNSLSMIDVVADGETPMISLASSAKIIEPVDAKRHWVFKTPQ
                     TDAMMASAIAEHASAHGVKTIAFIGQADALGETFYTEVAKFAQLHHINMVASERFNRT
                     DPSVTGQVLKILATHPDAVVVGAAGTPAALPPKTLRERGYKGLIYHNHGVGNNDFLRV
                     CGADCNGTFLPASPVLVAAQLPADHPAKRLALDYITRFEALRGPGSVSAFGSYTWDAG
                     MLLGNAIPIALKAGAPGTPEFRRALRDALEATHGLADTNGVVNMSATDHLGLDQRARV
                     MVEIKNAKWVYQPR"
     gene            4284..5165
                     /locus_tag="VHV99_27880"
     CDS             4284..5165
                     /locus_tag="VHV99_27880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX3639234.1"
                     /translation="MSQAPHSNDAFYAQYQSLQLHRHPHGVLEVVMSGEGANKSGLAT
                     ANARMHFELAEIWRDIDRDPETRVAIIRGEGKGFSAGGDLQLVEDMATDFDVRARVWR
                     EARDLVYNVINCSKPIVSAMHGPAVGAGLVAGLLADISIATKSARIIDGHTRLGVAAG
                     DHAAIVWPLLCGMAKAKYYLLLCEPVSGEEAERIGLVSLAVDENDLRPKAFEVAQKLA
                     NGSQTAIRWTKYALNNWLRSAGPAFDTSLALEFMGFAGPDVREGVNSLRERRAPDFGG
                     PDPWSGQPRHTPGDGRS"
BASE COUNT         1059 a         1696 c         1693 g          854 t
ORIGIN      
        1 cggcctcggg cttgcgtttc ggctgcggcg cgggcgcgcc cgcgcccgcg gccgcattgc
       61 gctcgatgcg ctccggcttc aggagcgcga cctgcacggg cacatgttcg tcgttcgatg
      121 gattcagcgt cgcgcggttg cgctcgaccc attgcgccgc aatccagtgc aggacggcca
      181 ccaccagcag aacggcaagc catcgccata cccgcaaacc ggcgcgcggc ccggcgggcg
      241 gcgtgcgatc agagcggcgt gtggcaggag gtgaggacat gggcgaagaa ttcgaacgga
      301 cggtcggtaa cgggataggc cggaggtgct cacggagcgt cgcgtgtcgg caaccgtaag
      361 agccgtcgag ccggagcgct gaatgggcag cgatcggttt cgaccgcccg caggcgaacg
      421 atgttcccgg actcagtcca tcctcagcgc atcggcatca gcacatcagc acatcagcac
      481 atcagcacat cagcacatca gcgtggcgcc gcactataac ccagctcgta cgacagcttc
      541 tgcgcacatt cgcgcagcgc tgtatcgatc tcgccgcccc agcgaatgtc gaacgcgcct
      601 tcgtgaccga gcgcgatcag gccgagcgcc aggtcgccgg tcgagtcgaa caccggcatg
      661 cagaatgcgt ggatggtcgg cagcaacatg ccttccacac gcgctgcttc gtgctgccgc
      721 acgtcggcca gcacccgttc gacctcgtcg cgcgtgcgcg gcccgcccat ctgcgacgaa
      781 cggcgcgaat cggctagttc acgctcgagc atcgcggcgg tcttgctcga cggcagatag
      841 gcggcaaaca gtagaccggt ggcggaactg agcagcggca tcacgtcgcc gagcttgagc
      901 gacgccttgg cgggatggct cgattccatc cagtgaacca tcgttggccc ctggttgccc
      961 cacaccgcga tgcccaccgt cagatcgagc cggtcgcgca gttcggcaag cgcaatgcgc
     1021 gcgagtttca cgccatcgac gcgggcaagc cgcgcgagcc ccaattgcaa tgcgaagccg
     1081 cccagttcgt agcggcccga cagcggatcc tgcgccacga cgccgaggcg caggaaactc
     1141 acgaggtagc ggtgtgcttt cgccggactc atgcccgcac gttgcgccag atcgcgcagc
     1201 atcatggcgc gcggttcgtg ggtcagcacc tcgagcaatc tgaagccgac ttcgatcgac
     1261 tggatgccgg agcgcagctt ttcctcgccc gtctcgacgc tttcgtcttc ggattcgggt
     1321 gcttcgagcg gttcagtgga gccggtgtcg gtacggatcg cgccggagcg ggcaattgga
     1381 ggcatgagcg aggcgcatgg agcgcgttca aaagacagtg agaaaggcgt gtcgcggata
     1441 acgctacttg ggccgcgccg attcaccatc gtagaataga ttccttctat cgtcactaac
     1501 ggttcacgcc tctatgaaac ttgccacgct gaaggacggc acgcgcgacg gtcagctgat
     1561 cgtcgtgtcc cgcgacctgc acaccgcggc cgtcgccgac gcgatcgcgc ccaccatgca
     1621 gcgcgtgctc gacgactggg ccttctacgc gccgcaactg caggacctgt acgacgcgct
     1681 caaccagggc cgcgcgcgca acactttccc gttcgacgcc agggaatgca tggcgccgct
     1741 gccgcgcgcg ttccaatggg ccgacggttc gtcgtacgtg aaccatgtgg aactggtgcg
     1801 ccgcgcgcgt ggcgcggaaa tgccgccgga attctggacc gatccgctga tgtaccaggg
     1861 cggcagcgac gacttcatcg gtccgaaaga cgacgtactg tgcgcatctg aagaattcgg
     1921 tatcgacttc gaagcggaag tcgccgtgat caccaccgac gtgccgatgg gcgccacgcc
     1981 cgatcaggca ctccggagcg tgcgtctggt gacgctcgtc aacgacgtct cgctgcgcaa
     2041 tctgatcccc gccgaactcg cgaagggctt cggctttttc cagagcaagc cggccacgtc
     2101 gtttgcgccg gtcgcggtga cgcctgacga actcggcgag agctggcgcg aagggcgcgt
     2161 gcatcggccg atgatcgtcc actggaacaa caggaaggtc ggccagccgg acgccggcac
     2221 cgacatggtg ttccacttcg gccagctgat ctcgcatgcg gcgaaaacgc gcaacctgcg
     2281 cgccggcgcg attgtcggct cgggcacggt gtcgaacaag gatgcgaaac gcggctactg
     2341 ctgtatcgct gaaaagcgct gcctcgagac gatcgagcac ggcgcgccgc agaccgaatt
     2401 catgaagttc ggcgataccg tgagaatcga aatgttcgac gaagcaggca agtcgatttt
     2461 cggttcgatc gatcagggca tcgcgccgct ggattgaggc gctggttttg tgcggtacgc
     2521 ggggtcaaga tcgcgtccct ttgcgcttcg ctatctacga aagggtttcg cccgatgccg
     2581 ccgccacaac gcgccgctac actgactggc gcggcggcgg cggcctcggg gtagcgactg
     2641 tccgttgagc gcgaaacgag cgtgaatcag gggggaccaa cgcgaaccaa cgcgaagcag
     2701 cacgtaagca cgacaagcac gacacaagca gaacacaagc agaacacaag cagaacacaa
     2761 gcagaacaca agcagaactc aagcagaaca caagcagaac atcagcagtt catcaaaaac
     2821 gaaaaaccga cgtgaggaga cagcatgcag cgatttgaat cccttgaacg aaaaacccag
     2881 cgcgctcgcg catcgaacgt gcgcgcaacg acgtcgcggc gcgggctcgc ccgcctggcc
     2941 gccgcgctct gtgccacgct acccgccatc gctctcgcgc aggtgaagat cggccttgtg
     3001 ctgtcgctga ccgggccggc ggcgtcgctc ggcattcccg cgcgcgatac cgccacgctg
     3061 ctgcccaaac agatcggcgg ccagagcgtc gagtacatcg tgctcgacga cgcctccgac
     3121 accacgcagg ccgtgcagga cacgaagaag ctgatctccg agaatcacgt cgacgcgatc
     3181 atcggctcgt cgatcacacc gaattcgctt tcgatgatcg atgtggtggc cgacggcgaa
     3241 acaccgatga tctcgctggc ctcgtcggcg aaaatcatcg aaccggtgga tgcgaaacgc
     3301 cactgggtct tcaagactcc gcagaccgac gcgatgatgg catcggcgat cgccgagcat
     3361 gccagcgcgc acggcgtcaa aaccatcgcg ttcatcggcc aggcggatgc gctcggcgag
     3421 accttctaca ccgaggtcgc caaattcgcg cagttgcatc acatcaacat ggtggcgagc
     3481 gagcgcttca atcgcaccga tccgagtgtg accggccagg tcctgaagat tctcgcgacc
     3541 catcctgatg cggtggtggt cggcgcggcc gggacgcctg ccgcgttgcc gccgaagacg
     3601 ctcagggaac gcggctacaa agggctgatc tatcacaatc atggcgtcgg caataacgac
     3661 ttcctgcgcg tatgcggcgc cgattgcaac ggcacgttcc tgcccgcaag tccggtgctg
     3721 gtcgccgcgc aactgccggc ggaccatccg gccaagcgcc tcgcgctcga ctacatcacg
     3781 cgcttcgaag cattgcgcgg accgggcagc gtgtcggcgt tcggttcgta cacgtgggac
     3841 gccggtatgc tgctcggcaa cgcgatcccg atcgcgctca aggccggcgc gcccggcaca
     3901 ccggaatttc gccgtgcatt gcgcgatgcg ctggaagcga cacacggtct cgcggatact
     3961 aatggtgtgg tcaacatgag cgccacggat cacctcgggc tcgaccaacg ggcgcgtgtg
     4021 atggtcgaaa tcaagaatgc gaagtgggtt tatcagccgc gctaagtttg cactgaatag
     4081 gcgccggttt cgcgttgacg tagcgccgaa tgtgtcgggt gcaccggatg tgccgcatgc
     4141 acggaatgca cggaatgcac ggaatgcacg gaatgcaccc aagtgcccaa gtgcccaagt
     4201 gcccaagtgc caaagtgcca aagtgcccaa gtgccaccag gccaacggtt tcaacagacg
     4261 taccgataga cggattgcac accatgtctc aggcgcccca cagtaacgac gcgttttacg
     4321 cgcagtacca gtcgctgcaa ctgcaccgcc atccgcacgg cgtgctcgaa gtcgtgatga
     4381 gcggcgaggg cgcgaacaaa agcggacttg ccaccgccaa cgcgcggatg cacttcgaac
     4441 tcgccgaaat ctggcgcgac atcgatcggg atcccgaaac acgcgtggcg atcattcgcg
     4501 gcgaaggtaa gggtttttcg gccggcggcg acctgcaact ggtcgaagac atggcgactg
     4561 atttcgacgt ccgcgcccgc gtgtggcgcg aagcgcgcga tctggtctac aacgtgatca
     4621 attgcagcaa gccaatcgtt tcggcgatgc acgggccggc cgtcggcgca ggccttgtcg
     4681 ccggactgct cgccgatatc tcgatcgcca cgaagtcggc gcgcattatc gacggtcaca
     4741 cgcgtttggg ggttgccgcc ggcgaccacg cggcaatcgt ctggccactc ctgtgcggga
     4801 tggcgaaagc caagtattac ctgcttctgt gcgagccggt aagcggtgaa gaggccgagc
     4861 ggattggtct cgtgtcgctt gccgtcgacg aaaacgatct gcggcccaaa gccttcgaag
     4921 tcgcgcaaaa actggccaac ggctcgcaaa cggcgattcg ttggaccaag tacgcgctga
     4981 acaactggtt gcgctcggcg gggccggcat tcgatacgtc gctggcgctg gaattcatgg
     5041 gtttcgccgg gcccgatgtg cgcgaaggcg tgaactcgct gcgcgaacgg cgcgcgccgg
     5101 attttggcgg cccggacccg tggagcggac aaccgcggca cacaccgggc gatgggcgtt
     5161 cctgaaaccc ggagcccggc acagattgcg caaaagcccc gccggccggc acttgaggcg
     5221 ccacgaaagt ggggcaaaca gtggggcaaa cagcagccga aactaagcac ggctcgcggc
     5281 gccccgcgcg gtatgatcga at
//
LOCUS       DATASH010001052         5772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_181587,
            whole genome shotgun sequence.
ACCESSION   DATASH010001052 DATASH010000000
VERSION     DATASH010001052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5772
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181587"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VHV99_27885"
     CDS             <1..220
                     /locus_tag="VHV99_27885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEX3639235.1"
                     /translation="FVTAGSDHKALAFLTLGGVFVVMSMVWNSFVAWIAGSVTRRFSG
                     KPSVKKWLDRGVGSAFVGLGIKLATASR"
     gene            324..1181
                     /gene="htpX"
                     /locus_tag="VHV99_27890"
     CDS             324..1181
                     /gene="htpX"
                     /locus_tag="VHV99_27890"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007581962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEX3639236.1"
                     /translation="MFNWVKTAMLMAAITALFIVIGGMIGGSRGMMIALVIALGMNFF
                     SYWFSDKMVLRMYNAQEVDENSAPQFYRMVRELSTRAGLPMPRVYLINEDAPNAFATG
                     RNPEHAAVAATTGILRVLSEREMRGVMAHELAHVKHRDILISTVSATMAGAISALANF
                     AMFFGGRDENGRPSNPIASIAVALLAPIAGALIQMAISRAREFEADRGGAQISGDPQA
                     LASALDKIHRYASGIPFPTAEQHPATAQMMIMNPLAGGGIANLFSTHPATEERIARLM
                     EMARTGRFE"
     gene            1432..2871
                     /gene="rsmB"
                     /locus_tag="VHV99_27895"
     CDS             1432..2871
                     /gene="rsmB"
                     /locus_tag="VHV99_27895"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEX3639237.1"
                     /translation="MTPKPSSRSASPSARPRESRLSMLHLAPESLGFSLDCAGQAVGA
                     VRLGAALPAALQSVFVSAPEGSAAAARGAVQDIAYRTMRRLATAEWLIAKLVKKAPPP
                     HVGHVLACALALLIDDEANAAYAPFTVVDQAVSAIAARREFSFAKGLVNAVLRNFLRE
                     REALLSAAQADEVARWNYPAWWIDAVKRAWPDAWQAVLAVGNTQGPLTLRVNARRSTI
                     DAYLQVLQDHHIPASRAGEYAIKLATPMPVDRIPGFNEGVVSVQDAGAQLAAQLLGVR
                     DGMRVLDACAAPGGKTGHLLELANLQLVALESDASRARRIGENLQRLKLEAEVRIGDA
                     GQPLKWHDDIDQPFDRILADVPCSASGIVRRHPDIRWLRRASDIPALVAEQRRILDAL
                     WPLVKPGGELLYVTCSIFPEEGELQAQWFGNKYQDAVRLDAPGQLLPSVARAPADTSA
                     GSHAGHVAEHSADSNSDHDGFFYARFQKR"
     gene            2868..3467
                     /locus_tag="VHV99_27900"
     CDS             2868..3467
                     /locus_tag="VHV99_27900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004553747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HEX3639238.1"
                     /translation="MTIKRFFPLRLAAVLWFALVVWLAVPGAAHADSIAVQRASLQSD
                     NTGWNLDARFDFDLNSNLEDAVNKGIPLYFTTDFELSRPRWYWFDEQPVSVSQSLRLS
                     FQPLTREYRVSSVSSGGLQLGFTTLKDALAVIKHITSWHVIDRTQVVVGETYNASVRM
                     QLDIALMPKPFQIDAVNNRDWSLSSDWKRFTFTVTERAK"
     gene            3457..>5772
                     /locus_tag="VHV99_27905"
     CDS             3457..>5772
                     /locus_tag="VHV99_27905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEX3639239.1"
                     /translation="MLNRVRRATSVSSIVVRVLVSTVAVTAVLLLVLLAAASANTEFF
                     DRYYQWLYAANLVVAMIFLLVVTALVIIIAVRLRKGKFGTRLLAKLAFFMALVGVVPG
                     GIIYVVSYQFVSRSIESWFDVNVETALTSGLNLGRGMLDASLSDLQTKGRLMSEQLAS
                     ADAAGTTLTLLRLRDQFGVQDATIVEPTRSMSGATPDMHVVAQASGNYATLVPSDLPT
                     PMMIDQARGRGYAAIEGEVDGDPAAHGSKGALRLRIVQRIPDSNASLLQPTERFLQLT
                     QPVSPSLARNADAVQRAYREYQEKALGRTGLRKMYIGTLTLALFLATFIAMMLALALG
                     NQLARPLFLLAQGTKEITEGDYTPKREIKSRDELGFLTQSFNAMTRQLSEARAAVENN
                     RVALEHSKAYLESILANLTAGVFVFDRQFRLTTANRGAERIFRQQFQEVLGSSLDQIS
                     VLSEFGGMVRKAFADREAASGDGHDDRGHWQQQFSVQVPGETEPLTLLVRGARLVSAT
                     DRDAEDMQTSGYVVVFDDISDVISAQRSIAWGEVARRLAHEIKNPLTPIQLSAERLQM
                     KLADKLSPSDADVLKRGATTIVNQVAAMKQMVDNFRDYARTPPAVLAHLQLNELVSEV
                     LTLYGIEEGKGAIQVELADLPAIRGDATQLRQVIHNLLQNAQDAVADVQHPRVLLETR
                     TVEYGDPDAEGKVHVAVRLTVSDNGPGFPARILTRAFEPYVTTKAKGTGLGLAMVKKI
                     VDEHGARIDVRNRMKAGDVIEGAQISILFLQL"
BASE COUNT         1005 a         1817 c         1837 g         1113 t
ORIGIN      
        1 attcgtcacg gcgggcagcg atcacaaggc gctggcgttc ctcacgctcg gcggggtgtt
       61 cgtcgtgatg agcatggtct ggaacagttt cgtcgcgtgg attgcgggca gtgtcacccg
      121 gcgcttctcc ggcaagccgt cagtgaagaa gtggttggat cgcggcgtcg gcagcgcatt
      181 cgtcggcttg ggcatcaagc tcgccactgc atcaagatga ttgaattttc cccacgcgcc
      241 catatctaac atttcgctta caatcagtcg ccgcgatcat gcggcgcgga tatgaacccg
      301 cgttagggca aggagttgca gacatgttca attgggtcaa aaccgcgatg ttgatggccg
      361 cgatcacggc ccttttcatc gtgatcggcg ggatgatcgg cgggtcgcgc ggcatgatga
      421 tcgcgctcgt gatcgccctt gggatgaatt tcttttcgta ctggttctcg gacaagatgg
      481 ttctgcgcat gtacaacgcg caggaagtcg acgaaaacag tgcgccgcag ttctatcgta
      541 tggtgcgcga gctctccacg cgcgcgggct tgcccatgcc gcgcgtctac ctgatcaacg
      601 aagacgcgcc gaacgcgttc gctaccggcc gtaacccgga acatgcggcc gtcgccgcga
      661 ccaccggcat cttgcgcgtg ctgtccgagc gcgaaatgcg cggcgtgatg gcgcacgaac
      721 ttgcgcacgt gaagcaccgc gacattctga tttcaaccgt ctcggccacc atggcgggcg
      781 ccatttccgc actggcgaac ttcgcgatgt tcttcggtgg ccgcgacgaa aacggccgtc
      841 cgtcgaatcc gatcgcaagt attgccgttg cgttgctcgc accgatcgcc ggcgcgctga
      901 tccagatggc aatttcgcgt gcgcgtgaat tcgaagcgga ccgtggcggt gcgcagattt
      961 ccggcgatcc gcaggcgctc gcctcggcgc tcgacaagat ccatcgttat gcgagcggta
     1021 tcccgttccc tacggccgag cagcatccgg ccaccgcgca gatgatgatc atgaatccgc
     1081 ttgcgggcgg cggcattgcg aatctgttct cgacgcaccc ggccaccgag gaacgcatcg
     1141 cgcgtttgat ggaaatggca cgtacggggc gcttcgagta acggtgctct gattggcgtg
     1201 acgagagtgg cgagcccgcg tggctcgcct ttttgttcgc gcgcaggcaa agccgagtcc
     1261 gatgccgctt cgcttatgcc ccgctgcggg tccttgcccg attgctcgcg ctccgcttgc
     1321 cgtcgctacg gtagtcggct gtcgccgccc tcggcggcct ccaagccgcg ccacgctaca
     1381 atgtgtgctg tttgcgccgt gcgcaccgcg cggcctgcct tagcctcttt catgactcca
     1441 aagccttctt cgcgttctgc ttccccatcg gcgcgtccgc gcgaatcccg tctgtcgatg
     1501 ctgcatctcg cgcccgaatc gctcggtttc tcgctcgact gcgcgggcca ggccgtcggc
     1561 gccgtgcgcc tcggcgcggc tttgccggcc gcgctgcaat ccgtcttcgt ttccgcgcct
     1621 gaaggcagcg ccgccgctgc acgcggcgca gtgcaggaca tcgcgtaccg caccatgcgg
     1681 cgtctggcga ccgcggaatg gttgatcgcg aagctggtga agaaggcgcc cccgccgcac
     1741 gtcggccatg tgctcgcttg cgcactggct ttgctcatcg acgatgaagc gaacgccgcc
     1801 tacgcaccgt tcacggtggt cgatcaggca gtgagcgcaa tcgccgcgcg ccgtgagttc
     1861 tcgttcgcaa agggcctcgt caacgccgtg ctgcgtaatt tcctgcgcga gcgcgaggcg
     1921 ctgctgagcg ccgcgcaagc cgatgaagtc gcgcgctgga actatccggc atggtggatc
     1981 gacgcggtga agcgggcgtg gcccgacgca tggcaagcgg tgctcgccgt cggtaatacc
     2041 caaggtccgt tgacgctgcg cgtcaacgcg cgccgctcga ccatcgacgc ctatctgcaa
     2101 gtactgcaag accatcacat ccccgcaagc agagccggcg aatacgccat caagctcgcc
     2161 acgccgatgc cggtcgaccg cattcccggc ttcaatgaag gcgtcgtctc ggtgcaggac
     2221 gctggcgcgc aactcgccgc gcaactgctc ggcgtgcgcg acggcatgcg cgtgctcgac
     2281 gcgtgcgcgg cgcctggcgg gaagaccggc catctgctcg aactcgccaa tctgcaactt
     2341 gtggcgctcg aaagcgatgc gtcgcgcgcg cgccgcatcg gtgaaaactt gcagcggctc
     2401 aagctcgaag cggaagtgcg cattggcgac gcaggccagc cgttaaaatg gcatgacgac
     2461 atcgaccagc cgttcgaccg cattctggcc gacgtgccgt gttcggcctc gggcatcgtg
     2521 cggcgtcatc cggatattcg ctggctgcgt cgtgcgtcgg atatccccgc gctggttgcc
     2581 gagcagcgcc gcattctcga cgcgctatgg ccgctcgtta agccaggcgg cgaattgctc
     2641 tacgttacgt gttcaatctt tcccgaagaa ggcgagttgc aggcgcagtg gtttggaaac
     2701 aagtatcagg atgcggtacg attggacgcg ccggggcaac tgttaccctc agttgcccgc
     2761 gcgcccgccg acacatcggc cggttcccat gccggacacg tcgctgaaca cagcgccgac
     2821 tcgaactcag accacgacgg atttttctac gcgcgcttcc agaaacggtg accatcaaac
     2881 gcttcttccc gcttcggctc gctgccgtgc tctggttcgc gctggttgta tggctcgccg
     2941 tgcctggcgc ggcgcacgcg gattcgatcg ccgtgcagcg tgcttcgctg caatcggaca
     3001 atactggctg gaatctcgac gcgcgtttcg acttcgacct gaacagcaat ctcgaagacg
     3061 ccgtcaacaa aggcattccg ctgtacttca cgaccgactt cgaactgagc cggccgcgct
     3121 ggtactggtt cgacgaacag ccggtgagcg tgtcgcagag cctccgcctg tcgttccagc
     3181 cgctcacgcg tgagtaccgc gtgtcgagcg tgtcgtcggg cgggttgcag ctcggcttca
     3241 cgacactgaa ggatgcactc gccgtgatca agcacatcac gtcgtggcat gtaatcgatc
     3301 gcactcaggt tgtggtcggc gagacgtaca acgcgtcagt gcgcatgcaa ctcgacatcg
     3361 cgctgatgcc caagccgttc cagatcgacg cggtgaacaa ccgcgactgg agcctttcgt
     3421 cggattggaa gcgctttacc ttcacggtga ccgaacgtgc taaatagagt gcgccgcgcc
     3481 accagcgtca gcagcatcgt cgtgcgcgtg ctggtgtcca cggtcgccgt gacggccgtg
     3541 ctgctgctcg tgctgcttgc agcggcgagc gcgaataccg aattcttcga ccgctattac
     3601 cagtggctat atgcggccaa cctcgtggtc gcgatgatct ttttgctggt ggtgacggcg
     3661 ctcgtcatca tcatcgctgt gcggcttcga aaaggtaagt tcggcacacg gctgttagcg
     3721 aaactcgcgt tcttcatggc gctggttggc gtggtgccgg gcggcatcat ctacgtcgtc
     3781 tcgtatcagt tcgtgtcgcg cagtatcgag tcgtggttcg acgtgaacgt tgaaacggca
     3841 ttgacctcgg ggcttaatct cggccgtggc atgctcgacg cgtcgctgtc cgatctgcag
     3901 accaaggggc gcctgatgtc cgagcagctg gcgagcgcgg atgccgccgg cacgacgctc
     3961 acgctgttgc ggctgcgcga tcagttcggc gtgcaggacg cgacgatcgt cgaacccacg
     4021 cgcagcatgt cgggcgcgac gccggatatg cacgtcgtcg cgcaggcgtc cggcaattac
     4081 gcgacgctcg tgccgagcga cctgcccacg ccgatgatga tcgaccaggc gcgtggccgt
     4141 ggctatgcgg cgatcgaggg cgaagtcgac ggcgatcctg ccgcgcatgg cagcaagggc
     4201 gcgctgcggc tgcgcatcgt gcagcgcatt ccggattcga acgcgtcgct gctgcagccc
     4261 accgagcgct tcctgcagct cacgcagccg gtctcgcctt cgctcgcacg caatgccgac
     4321 gcggtgcagc gggcctatcg cgagtatcag gagaaggcgc tggggcgcac cggcttgcgc
     4381 aagatgtaca tcggcacgct cacgctggcg ctgttcctcg ccaccttcat cgcgatgatg
     4441 ttggcgcttg cgctcggcaa tcagctggcg cggccgttgt tcctgctcgc gcagggcacg
     4501 aaggaaatta cggagggcga ctacacgccg aagcgcgaaa tcaaatcgcg cgacgaactg
     4561 ggctttctca cgcagtcgtt caacgcgatg acgcggcagt tgtccgaagc gcgcgcggcg
     4621 gtcgagaaca accgcgtagc gctcgaacac tcgaaggcat atctggaaag cattctcgcg
     4681 aacctgaccg cgggcgtgtt cgtattcgac cggcagttcc gtctcactac ggcgaaccgc
     4741 ggcgcggaac gcatcttccg tcagcagttc caggaggtgc tgggttcgtc gctcgatcag
     4801 attagcgtgt tgagcgaatt cggcgggatg gtgcgcaagg cgtttgccga tcgcgaagcc
     4861 gcgagcggcg acgggcacga cgaccgcggc cactggcagc aacagttctc ggtgcaggtg
     4921 ccaggcgaaa ccgaaccgct caccttgctc gtgcgcggcg cgcgccttgt gtccgccact
     4981 gaccgcgacg cggaagacat gcagacttcc ggctacgtcg tcgtgttcga cgatatttct
     5041 gatgtgatct ccgcgcagcg ttcgatcgcc tggggcgagg tggcgcgacg tctcgcgcat
     5101 gagatcaaga atccgctcac gccgattcag ctttccgccg agcgtttgca gatgaagctg
     5161 gccgacaagc tgtcgccatc ggatgccgat gtgttgaagc gcggcgcgac caccatcgtc
     5221 aatcaggtcg cggcgatgaa gcagatggtc gacaatttcc gcgactatgc gcgcacgccg
     5281 ccggcggtgc tcgcgcatct gcaactgaat gaactggtca gcgaggtgct cacgctgtac
     5341 ggtatcgagg aaggcaaggg cgcgattcag gtggaactcg cggatttgcc ggcgattcgc
     5401 ggcgacgcga cgcaattgcg tcaggtgatt cacaacctgc tgcaaaatgc gcaggacgcc
     5461 gtggccgatg tccagcatcc gcgtgtgttg ctcgagacga ggacagtaga atatggagac
     5521 cccgacgcgg aaggcaaagt acacgtcgcg gtgcgtctga cggtgtcgga taacggaccg
     5581 ggcttccccg cgcgtatcct cacacgtgca ttcgaacctt acgtgacgac aaaggccaaa
     5641 ggaacgggcc tcggtcttgc gatggtcaag aagatcgtcg acgaacacgg cgcgcgcatc
     5701 gacgttcgca accgcatgaa agcgggcgat gtgatcgaag gcgcgcagat ttcgatcctc
     5761 ttccttcaac tg
//
LOCUS       DATASH010001053        17673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_773087,
            whole genome shotgun sequence.
ACCESSION   DATASH010001053 DATASH010000000
VERSION     DATASH010001053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 17673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17673
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773087"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..597)
                     /locus_tag="VHV99_27910"
                     /pseudo
     CDS             complement(1..597)
                     /locus_tag="VHV99_27910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612148.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            983..1765
                     /locus_tag="VHV99_27915"
                     /pseudo
     CDS             983..1765
                     /locus_tag="VHV99_27915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393273.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
     gene            1931..3217
                     /locus_tag="VHV99_27920"
     CDS             1931..3217
                     /locus_tag="VHV99_27920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3639240.1"
                     /translation="MKLGKYRYVVAGLLFTAGAINYMDRAALGIVAPIVGKSLHLSPS
                     QLGIVFSTFFVGYSVFSFLGGYFSDKWGTLRVFTWAMTVWSLFCGLTAAATGFVSLLI
                     YRIFFGIGEGPMSSNTNRTISNWFPRHEAATVIGFTFSGQTLGNAIAGPVVGLIALDF
                     GWRASFVVIGILGLVWIVCWRLFVTETPAESRRVGDEERRLIDESRALAEAHNVEEDG
                     MTLGAYLRRPSTLALGAGLFAVNYTQYVFISWLPSYLTNVMHLDLRSMSIITSIPWIC
                     GALGYFGGGVIGDFIYRRMDDPIMARKLVATIPLALSGVAVLGITSAGSLTAAVALIA
                     AALFFLTGSCQSIWAIQHEILPRHRQGGVGGFIHFLSNLSGIIGPALTGFLIQYFGGY
                     SSAFLFGAVIDFAGVLAMVLCIRGRLSIPVAATSVE"
     gene            complement(3278..4360)
                     /locus_tag="VHV99_27925"
     CDS             complement(3278..4360)
                     /locus_tag="VHV99_27925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEX3639241.1"
                     /translation="MDILKSCTVLVSNSGDGDLSTYEFGSSTPTLRAGERHEIGSQVM
                     PLALSADQKAIYAATRGASPSLVKCRLDTRTGQLADEYRVAINASLASIATDRSGRFL
                     FGASYGGHEVIVYSLARLEAGDSTPLQTLSGVRNAHAVVVSDDNRYVYATSLGNDELV
                     CCTIDENQAQPLKVVDTVSFEAGFGPRHMRLSPGGEWLYVLSEFRAVVAVFRRDAATG
                     TLALHGISPRASVLAGLNHGFARPPSTEPQPDPATLTSLIWAADIHVRPDGLFIYISE
                     RTTNRLLVLRVRPDGMLEHASHVETEAQPRGFCIDPSGRFLVVCGEKSTHVSLFSIDT
                     DSGALALCSRSYGGRGANWVEIVARM"
     gene            complement(4672..5334)
                     /locus_tag="VHV99_27930"
     CDS             complement(4672..5334)
                     /locus_tag="VHV99_27930"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEX3639242.1"
                     /translation="MTSRIVLLHATPVAMQPIHSAFAEVWPEAQTINLLDDGLTIDRA
                     RENELSEPLIERFVRFGRYGYDMGADAILITCSAFGPAIDRLAAALPVPVLKPNEAMF
                     QAAVSYGLRIGMLATFGPAVITMTEEFDEYVAQVGSKAELKTVLVDEAIDELRRGNVE
                     RHNTLVAARAPELADCDVVMLAHFSTSRAAAQVRDKISVPVLTAPQAAVERMRELVET
                     GR"
     gene            complement(5358..5990)
                     /locus_tag="VHV99_27935"
     CDS             complement(5358..5990)
                     /locus_tag="VHV99_27935"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX3639243.1"
                     /translation="MIAALREHGIDATIGWDPTTVIEARLRKGDTADAIIVTDSAMKN
                     MVDAGWVAARHCLPLVESSIGVAVKRGAPRPDISSVQNFFDALLKSRSVAYSLSGASG
                     IYLQQLLRENGHLEEINRRATPIDKGLVAERLVSGEADIAIQQISELSAVDGIDIVGP
                     LPAKIQQIVSLSVGICSASVDRPVAHDLLKVLTSGHAKAIYERNGLTARW"
     gene            complement(6100..7428)
                     /locus_tag="VHV99_27940"
     CDS             complement(6100..7428)
                     /locus_tag="VHV99_27940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012404888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3639244.1"
                     /translation="MATTNSLDAHSTLEHRTIRKVSRRIIPFLIILYFLSFLNRVNVA
                     FAGLTMNHDLGFTNAIFGTGAGVFFIGTFIFGTPSNIALHRIGARRWIALMMLAWGSL
                     SAANALATNPTSFYLLRFFIGVAEAGFFPGVILYLSFWYPTRHRAFVTAIFMAAAPLS
                     NMIGSPISGALLQYTNGVAHLHGWQWLFIVEALPTVVLGFVALVYLTDKPEDAQWLEQ
                     DERRWLTQELEKEHSEKAARSASNVWVAFKDIRVWLLALVYSGTSMGLYAIGFWAPML
                     LKQFGFSFVELGIINAIPNLVAVVGMVYWGRRSDRQRERRLHVAIACLAGAFGMVVAG
                     QAASALVLVIALSVANFGINAAKPPFWSMPTLFLSSSAAAAGIGLINSVGSSIGGTVG
                     QMVIGKLRDLSGNYSSGLYFVSATLVLSAVIAVLFGRPAASKVEMRSPSK"
     gene            complement(7499..8167)
                     /locus_tag="VHV99_27945"
     CDS             complement(7499..8167)
                     /locus_tag="VHV99_27945"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HEX3639245.1"
                     /translation="MTRPVPCAVHASEEAKIREEICVTGASLYTRGYTVGSAGNISAR
                     LEDGWLITPTDACLGRLDPARIAKVDLAGNLVSGDRPSKTLALHRGVYARNRDVRGIV
                     HTHSTHLVALTLAGVWSEADVLPPITPYFVMKVGHVPLIRYRRPGDPQVAQQIAALAE
                     SVRGVMLERLGPVVWERSVSQASYAIEELEETARLWLMSNPRPAPLDAAALEELRTVF
                     GARW"
     gene            complement(8164..9477)
                     /gene="otnK"
                     /locus_tag="VHV99_27950"
     CDS             complement(8164..9477)
                     /gene="otnK"
                     /locus_tag="VHV99_27950"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HEX3639246.1"
                     /translation="MLTHSPRPLLGCIADDFTGGTDLACTLVQGGMRTFQTIGAPAST
                     DPIDADALVVALKSRTVDTATAVAQALAALDWLRAQGCRQFFFKYCSTFDSTDAGNIG
                     PVADALLDALSAGDDAPFTIACPAFPENGRTVYRGHLFVGDSLLNESGMEHHPLTPMT
                     DANLVRVLQRQTRSKVGLVRYDAVAQGVDAVRASIDALRREGVRIAIADAVSDAAIRT
                     LGEACADVPLITGGSGIALGLPENYRRAGLLVDAAAAGDLPSIEGRSAVLAGSASMAT
                     NAQVAAWCESRPAFRIDPLAAARGEPVVERALAFAREHMEARVPQPVLIYATAAPQDV
                     KAAQRELGAAEAGHLVERTLAAIARGLRELGVRKFVVAGGETSGAVVQALGVKMLRIG
                     RQIDPGVPATATIGESPLALALKSGNFGTVDFFAKALHQLDGGMR"
     gene            9816..10559
                     /locus_tag="VHV99_27955"
     CDS             9816..10559
                     /locus_tag="VHV99_27955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008346923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEX3639247.1"
                     /translation="MLRQLRRDKVLSVQQLMAAFNCSHMTVRRDIAALEQAGLVFTVP
                     GGVRIASHLGNEPSHQSKFSVEQPQKQAIARVAAQSLRAGMTIYLDAGTTMLAIVSHI
                     VALSDMTVITNDFEIVRELSGATHVSVIHTGGQLDHANLSSVGPLAATTLRQVVLDVA
                     FISASSWDLRRGVTTPSAPKVEVKLAAMEAASRTVLAVSSSKYGTFGLYKVAALDRFD
                     LIITDDGLAPAAVLGMRQSGVELELASVG"
     gene            10596..10988
                     /locus_tag="VHV99_27960"
     CDS             10596..10988
                     /locus_tag="VHV99_27960"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX3639248.1"
                     /translation="MSIDGEMNIHAISITTTEVSDSEGMDTVLPADLPVDRVIADGAY
                     YSIERTEALSSAGVTPVIPPSAHVVVHGNDQTRWHDQIVKYIDEKGIYGFLARCMGTH
                     ARPNKVAPERCVWGADWPHPDLLNGMPR"
     gene            complement(11150..12034)
                     /locus_tag="VHV99_27965"
     CDS             complement(11150..12034)
                     /locus_tag="VHV99_27965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3639249.1"
                     /translation="MDTRFLQSFIAVIESGSIAEAARRLDLAPTTVAQQIRALENDIG
                     SKLLTRAGRTVKPTVVGARILDRTRDLLRDVRDLRSAASDTGLPAGPLRLGATPTALM
                     GLLPPFLRRWKQTHPGIPIYIEPGTSVTLLDGVMSGELDAAIIVHPTFELPKTCSWRL
                     LREEPLILLTQTRLRVKDALATVAEEPFIRYDRRVVAGKMADEYLHAKGVRPNVQFEL
                     DGIEHIAKLVAEGFGVSVLPDWPVIGVPDPQLRRWSLPQPCPTRRVGLGWLRASVRSQ
                     LVEEFAHIACSEHAKVSR"
     gene            12146..13270
                     /locus_tag="VHV99_27970"
     CDS             12146..13270
                     /locus_tag="VHV99_27970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010926593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX3639250.1"
                     /translation="MPIIKSIEVCAARVPLDKVTSFSNRTVTERHYGLVKVRTADGIE
                     GVGFCYVGSSAGDLFRVAVEQLLAPVILGRDSLAVEGLWKDMYQESLLQGRMGTVMRA
                     LSAIDIALWDINSKSAGLPLHKYLGAVDLDTVAAYASGGYYVEGKTAEMVGEEMASFV
                     AKGFKAVKMKTGRWSPLGEEARVKAAREAIGPDVELMLDCNNGWIDTVQAMQYLSRIE
                     QYNPYFIEEPFSPDDIESHARLAKLTRIPVATAEIGYGRWYHKQLLDMGGAAILQTDA
                     AVCGGITEWKRIAALSAGYGVQVCPHWFHDVHAPLVAATPNARYVEFFWDDQVLNFRQ
                     LIDRQLEQREGRVVLHQTPGLGFGFDEKQVAKYGRWTIVS"
     gene            13527..14897
                     /locus_tag="VHV99_27975"
     CDS             13527..14897
                     /locus_tag="VHV99_27975"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3639251.1"
                     /translation="MQQSNEYLIVRKIFWRVIPLLMVLYVVSYIDRINVGFAALTMNH
                     DIGLNEYTYGWGAGIFFFGYCLFEVPSNLMMVRFGARRWIARILFTWGLLSCAMALVA
                     GPTSFLVLRFLLGVAEAGFFPCVILYLTFWFPARYRARIIATFSLAIPVSIAVGAPLS
                     TLILELDGMLGVKGWQWLFIIEGIPAVLGTFFVLRMLTDRPEEAQWLTTQEKAWLADE
                     LNKDIVASNEHNVSALRRAFTDPAVLTLALVYFCAIGANLGLSFFLPQIIKAQGYSTM
                     QTGLITSVPYIAGCVGMLAIGFLSDRFNERRWFLAATMVLTAGGLAVAGALGASVWSI
                     AAVSVAAIGILGCKGPFWALPATYLSGQAMAGGIAFINAMGNLGGFAGPYLVGFFKSS
                     SNSYASGLYALAALVQRPAITFNKCLHDLSWWHEPRSSCNASEAGQEGPGGTAGADQA
                     HDGSTA"
     gene            14800..15882
                     /locus_tag="VHV99_27980"
     CDS             14800..15882
                     /locus_tag="VHV99_27980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEX3639252.1"
                     /translation="MSRGRHATPVKLVRKDREELLALIRRTTAAQRDVTRARIALMAH
                     EGYSGAAIAQELGVSVQTVSLWRQRVARQGARGIREGERSGRPPRITQEARLQLIALA
                     CEVQDAHGRVTPTLDEIMARAVERGIVAQISRSHVQRILQAGDVRPHRVQQCLHSPDP
                     AFREKVNVICRLYRKAPKNAVVLSIDEKTGVQAIERKHPGRAPAAGRLRRREFEYIRH
                     GTQALIAALDVHTGQVLGSCRDRRTQDDLVAFMDSVAAAYPGKQVHVIWDNLNTHRAH
                     AVWQAFNARHSERFHFHFTPLHASWVNQIELWFAAYTRRVLRHASHLSTAHLRERTAH
                     FIRQRNQTARPFRWTFRGYPLQTGAS"
     gene            15895..16194
                     /locus_tag="VHV99_27985"
     CDS             15895..16194
                     /locus_tag="VHV99_27985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639253.1"
                     /translation="MPALPAKHHASELQRQLRALLGHDQIVTQAYGRHLLIKRLDDQE
                     PTVVARLSELSRNRYGAAFRSHSGRWEPLPGSGSLDEIAQVVVTLLQPYLQPDNY"
     gene            complement(16527..17639)
                     /locus_tag="VHV99_27990"
     CDS             complement(16527..17639)
                     /locus_tag="VHV99_27990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013094582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX3639254.1"
                     /translation="MLAVSLELAAAKWKVALHDGRREKPAVHTVAQPQAAARLQAVLD
                     LIETDKEKWSLPAGVRIVVSYEAGQDAFWICRALQAHGIECYVVDPASIPVERHRRRA
                     KTDRLDVIKLVINLRAWLRGERDRMHVVHAPSPEDEASRHLMRERGQLQKEVLQHRDR
                     MRKLLATLGCWDAVDHRAFADRLEREEVRCHDGAPLPQEMRERLLRECERLALVEQQL
                     AALEKTRQTNVPAPARQRCDALARLKGIGEVGASRLALELFWRDFNNRREVGACVGLV
                     PQPYDSGESQVDQGISKQGNRRVRALLIEMAWTWLRYQPGSALAKWFDQRTHGTGPNR
                     RVRRIAIVAVARRLAIALWRYLKDGVIPEGAHLKSA"
BASE COUNT         3413 a         5351 c         5299 g         3610 t
ORIGIN      
        1 gcttgtgccg ggcacggatg ccgtgctgag ccatcagctt gcgaacgcgc tccttgccca
       61 cgcgcacgcc gcgtgcaacc agttccttcc acatgcgcgg ccagccatat tcgcccttga
      121 cctgcgcgtg aatggccttg aggtgcacga gcagcgcatc gttgctgata cggcctctgt
      181 gcggcttatc acagccggtg cgctgttgac gctgatgata tccgctgggg ctgactccca
      241 gtgcttcaca cagcaccgtg accggccagt ggtgtcgatt ccggtcaatg aacgcgcact
      301 tcacatcaat tccttcgcga agtacgtcgc cgtttttttt gtgatgtcga gctccatctt
      361 caggcgagac acttccgcac gcaagcgcgc cagttccatc tgttctggac tcaccggctt
      421 ggctccggct cccctgagct tgccctcccg gtcggccttc acccagttgt acagcgtctg
      481 ttctgccatg cccagcgtcg ctgctaccgc cgccatgctt tgaccgtgct tgaccagccg
      541 aaccgcttcc agcttgaatt caagcgtgta ctgtgcacgc tttgccttgc tcatcatctt
      601 gactctcctt gcttgagttt acccgctcag caagggattc gtttttcggg ggcaagctca
      661 cagttcgctt cgttccggcc accctaaccg attcgccgat cccatttgca ccggagccgt
      721 caaaatcacg tctagtcagc ggatagcgca agcgttttca ttaccggggc caacgccggg
      781 ttcagtgctt catcgatttt ttcatcagcg actttgtatc gcccggaatt ctatcgtcga
      841 tctcctatcg cgacacaacc gacaaaaatt ttatcgtctg aatataccga tggatactgt
      901 tccagaacgt caagggctat cacacttcgt tgctagccgc catgacctga cacgcggccg
      961 acgtccagga ggagcgctga aaatgccaag aatcgaatcg atttccgtct acggggccgt
     1021 cgatccgctg gaacgtgtga cttcgttcgc tacgtcgaca gcatcgaaac gcgagtatgg
     1081 tgcgtcgtta aagtccgctc cagcaacggc atcgaaggta tcggtttctg ctgtgtcggc
     1141 agcgcggatg gcgagccgct acgtgtggca gtcgagggtt gctcgcgcca gttctcctcg
     1201 gaaaacgatt cttatgccgc tgaagggctc cggcagaggg catatcagga agcgctgctt
     1261 cagggccgcg ccggcactgt catgcgcgca atcaccatcc gcagcactgc cttatgggac
     1321 ctcaatgcgc gcacggatcg cttgccgcat cacaggtatc tgggtgcgag cgcactcgat
     1381 accgtgcccg catacgcgag tgatgggtat tacctggaag gcaagacgca ccagatgcac
     1441 ggcgacgaaa tggcaagcta tgccgaaatg ggtttcgagg cggtcaggat gaaggctgga
     1501 cgcctgtcgc catccgaaga caaggcccag cgtgcgcgcc gcgcgcgagg cggtcggccc
     1561 ggacgtcgcg ctgatgatcg acatcaataa cggctgccgc gacgtgaccg aggcgcgtca
     1621 atacgtgtgc cgctttgagc agtaccaccc gtacttcatc ggggagccat tttcgcccga
     1681 cgaggtggat caccacgggc gtctcctgaa gctcgcgctc gtgcccatcg aaacgggcga
     1741 gatcggctat caccgttggt atcactaaga acttctcgac aaggatgccg cggagattct
     1801 gcagaccgat gaccagtgaa atgacgggcg cagcgattcg cacggcctgc aaggcgacct
     1861 cggcggtaca tcgcggactg atccaaagtt tgaccgcggc acagcaaaat atatttttat
     1921 ggagacaaat gtgaagcttg gaaaataccg ctatgtagta gccggccttt tatttacagc
     1981 cggcgcgatc aattacatgg accgcgcagc gctaggcatc gtcgcaccga tcgtcggcaa
     2041 gtcgcttcat ctgtcgccct cgcagctcgg catcgtcttc agcactttct ttgtcggcta
     2101 ctctgttttt tcgtttctcg gcggctactt ttcggacaag tggggcacgt tgcgcgtctt
     2161 cacgtgggcg atgactgtct ggtcgctgtt ctgcgggctg accgcagctg ctactggttt
     2221 tgtttcgctg ctgatctacc ggatattctt cggcattggc gaagggccga tgagctcaaa
     2281 cacgaaccgc acgatatcga actggttccc gcgacacgaa gcggcgacgg tgatcggctt
     2341 taccttttcc gggcagacgc tcggcaatgc gatcgccggg ccggtcgtcg gcctgattgc
     2401 gctggacttc gggtggcgag cgtcgtttgt cgttatcggg atcctcggct tggtgtggat
     2461 tgtgtgctgg cgcctgttcg ttaccgaaac gcccgctgaa agccgtcgcg tcggcgatga
     2521 ggaacggaga ctgatcgacg agagccgtgc cctcgccgaa gcgcacaacg tcgaagaaga
     2581 cggcatgacg cttggtgcgt atctgcgccg tccaagtacg cttgcgctcg gcgcgggcct
     2641 tttcgccgtg aactacacgc aatacgtgtt catttcctgg ctgccgagct atctgaccaa
     2701 cgtaatgcat ctcgacctgc ggtcaatgag catcatcacg tcgatcccat ggatctgcgg
     2761 cgcgcttggc tatttcggtg gcggtgttat cggcgacttc atctaccgtc ggatggacga
     2821 cccgatcatg gcacgcaagc tcgtggcaac cattccgctt gctctatcgg gcgttgcagt
     2881 gcttggcatc acgtcggccg gttcgctaac cgcggcggtc gcactgattg cggcggcgct
     2941 gttctttctc actggctcct gccagtcgat ctgggcaatc cagcacgaaa tactcccgcg
     3001 gcaccggcag ggtggtgtcg gcggcttcat tcattttctg agtaatctgt ccggcattat
     3061 cggcccggca ctgacggggt tcctgatcca atatttcggc ggctacagca gcgcatttct
     3121 gttcggcgcg gtgatcgatt tcgctggcgt cttagcgatg gtcttgtgca tacgtggtcg
     3181 actatcgata cctgtcgctg caacgtccgt cgagtgaact tggtcagcta cgcatcaatc
     3241 cgaccggcgt gatgcgcagc agttctatac ttgatgtcta catccgcgca acgatctcga
     3301 cccagttcgc gccgcgaccg ccgtaactac gtgagcacag cgccagtgca cccgagtccg
     3361 tgtcgatgct gaacagcgag acgtgggtcg acttctcgcc gcagacgacg agaaaccggc
     3421 cggatggatc tatgcaaaag ccgcgcggtt gcgcttcggt ttcgacgtgg ctcgcatgtt
     3481 cgagcatgcc gtccggtctc acgcgcagca cgaggaggcg gtttgtcgta cgctcggaaa
     3541 tatagatgaa gaggccgtcg ggacgcacat ggatgtcggc agcccagatc agcgatgtga
     3601 gcgttgcggg gtcgggctgg ggttcggtcg agggaggtcg tgcgaagcca tggttcaagc
     3661 cggcaagtac gcttgcacgc ggtgatatgc cgtgcagcgc cagcgtgcct gtagcggcgt
     3721 cgcgccggaa cacggcaacg accgcgcgaa attcgcttag cacgtagagc cattctccgc
     3781 ccggcgacag ccgcatgtga cgcgggccga acccggcctc gaacgatacg gtgtcgacca
     3841 ccttaagcgg ctgcgcctgg ttctcatcga tcgtgcagca cactagctcg tcattgccga
     3901 gggaggtcgc atagacgtaa cggttgtcgt ccgacacaac caccgcatgt gcgtttcgaa
     3961 cgccggagag cgtttggagc ggtgtgctgt caccggcttc cagccgcgcg agactgtaca
     4021 cgatgacttc gtggccgccg tacgatgcgc cgaacagaaa gcggccagat cggtccgtcg
     4081 cgatcgacgc caggctcgca ttgatcgcga cgcggtattc gtcggcgagc tgaccggtgc
     4141 gcgtatccag cctgcatttc acgagtgacg gcgaggcacc tcgcgttgcc gcgtagatcg
     4201 ccttttggtc ggcggacagc gcgagcggca tcacctggga gccgatctca tgccgttcgc
     4261 ccgcacgcaa cgttggcgtc gatgagccga actcgtaggt cgacaggtcg ccgtcgcctg
     4321 agttcgagac tagtaccgtg cagcttttca aaatatccac gatgtttaat ccgatgggaa
     4381 ttgcgaatgg tggtcggaat cagctccgcc agcaggcatc gattttacgt accatttcgc
     4441 gcatacgcat gatgccatgc atgcgacgcg gtcaatacgg acacggaggt ctggctcgta
     4501 cctgaacggc tgcaagcgac gtagaaaagt gggtgtcggg ataagggcgg caagatccgc
     4561 aggtaggcaa gtcagcaacc agttcgactt gcctgtcgaa ggcatgagcg atttcgtctt
     4621 gtcgcgccgt cttgcgcgac cgtcggtaac acgacggcgc cctagcgagt tctaacggcc
     4681 tgtttccacg agttcgcgca tgcgctcaac ggcagcttgc ggcgccgtca gcaccggcac
     4741 ggagatttta tcgcgcacct gtgcggctgc gcgcgaggtc gaaaagtgcg ccagcatgac
     4801 gacatcgcag tcggccagct cgggggcccg cgccgccacg agagtgttgt ggcgctctac
     4861 attccctcgg cgcagttcgt cgatggcttc atcgaccaat actgtcttta gttcggcctt
     4921 gctgccaacc tgggcaacgt attcgtcgaa ctcctcggtc atggtgataa ctgcgggtcc
     4981 gaacgtcgcc agcatgccaa ttcgcagacc ataggagaca gccgcctgaa acatggcctc
     5041 attcggtttt agcaccggca ccggcagcgc cgccgccagg cgatcgatag cgggaccgaa
     5101 tgcagaacag gtgatgagaa tggcatctgc gcccatgtcg tagccgtacc gaccaaagcg
     5161 cacaaagcgc tctatcagcg gctccgagag ctcgttctcg cgggcccggt cgatggtcaa
     5221 gccatcgtcg agcaggttga tggtctgtgc ctcgggccac acttcggcga atgcactgtg
     5281 aatcggttgc atggcgacgg gggtggcatg cagcaaaaca attcttgatg tcatttgtac
     5341 tccattcaca agcgggttca ccagcgggct gtgagtccat tgcgttcgta gatcgctttc
     5401 gcgtggccgc tggtcagcac tttgagcagg tcatgtgcca ctggccggtc cacggaagcg
     5461 ctgcaaatgc cgacgctcag cgagacaatc tgctgtatct tcgcgggaag cggaccgaca
     5521 atgtcgatgc cgtcaaccgc actcaattcg ctgatttgct ggatcgcgat atccgcttcg
     5581 ccacttacca gcctttccgc aacaaggcct ttgtcgatag gggtggcccg acggttgatt
     5641 tcttcaagat gcccgttctc gcgcagcagt tgctgcaaat aaatgccgct cgcgccgctc
     5701 aatgaatacg ctacggaacg actcttcaac agggcgtcga agaaattctg cacggaagaa
     5761 atatccgggc gcggcgcccc gcgcttcact gccacgccaa tggaagattc gaccagaggc
     5821 aggcaatgtc gcgcagcaac ccatcctgcg tcgaccatgt ttttcatcgc gctgtcagtc
     5881 acgatgatcg catcggccgt gtcgcccttt ctcaggcgcg cctcgatgac cgtagtagga
     5941 tcccagccta tcgtcgcatc gattccgtgt tcgcgaaggg cggcgatcat gccgttgtca
     6001 aatgccgcgc ggacggctag cgcgctgaac aagccggcct tgtaagcatc agattgcatg
     6061 ggtattcacc tgtactgtta aaaagttcgc gttgccggcc tatttgctcg gcgaacgcat
     6121 ttccactttg gaggccgccg gtcgcccgaa caggacggca atgacagcag aaagcacgag
     6181 cgtcgcgctg acgaagtaaa gccccgaaga ataattgcct gacaggtcgc gcaatttgcc
     6241 aatgaccatc tgccctaccg tgccgccgat cgagctccct accgagttga tcaggccgat
     6301 gcctgccgcg gcggccgagc tcgaaagaaa tagcgtcggc atgctccaga acggcggctt
     6361 cgctgcattg ataccgaaat tggcgaccga cagggctatc accagcacca gcgcggaagc
     6421 cgcctggccg gcgacgacca tgccgaacgc gcccgcgagg caggcgatgg cgacatgcag
     6481 acgcctttcg cgctgacgat cggagcgccg cccccaatag accatgccga caaccgcaac
     6541 cagattcggg atcgcgttga tgatgcccaa ctcgacgaag ctgaagccga actgtttgag
     6601 cagcatgggt gcccaaaagc cgatcgcgta aagccccatc gatgtccccg aatagaccag
     6661 cgcaagaagc cagacgcgga tatccttgaa cgcaacccag acattggagg cgctccgcgc
     6721 cgctttttcg gagtgctctt tttccagctc ctgggtcaac cagcgccgct catcctgttc
     6781 gagccactgt gcgtcttccg gcttgtcggt cagatagacc agcgcaacga atcccagcac
     6841 aaccgtggga agggcttcga cgataaagag ccattgccag ccatgcaggt gagcaacacc
     6901 attcgtgtat tgcagtagcg cgccggaaat tggagacccg atcatgttgg ataacggcgc
     6961 cgccgccatg aagatcgccg tcacgaacgc gcgatgacgt gtcggatacc agaagctaag
     7021 gtacagaatg acgccaggaa agaagcctgc ctccgcgacg ccaataaaga agcgcagcaa
     7081 gtaaaagctc gtgggattcg ttgccagggc attcgctgcg gaaagcgaac cccacgcgag
     7141 catcatcaag gcaatccagc ggcgggctcc gatgcgatgc agagcgatat tcgacggcgt
     7201 gccgaagata aaggtaccta taaagaacac cccggcgcca gttccgaata ttgcgttggt
     7261 gaagccgaga tcgtggttca ttgtcagccc cgcgaacgcg acattcacgc gattcaggaa
     7321 gctgaggaag tacagaatga tgaggaacgg aatgatccgc cgcgacacct tgcggatcgt
     7381 tctgtgctcc agtgtcgagt gggcgtctag tgagttcgtc gttgccacag tcgttgtctc
     7441 cttgttattt gttcatgcag gccgctcgtg acagatgcct gcccgttgca tcggagcatt
     7501 accagcgcgc gccgaacacc gtgcgcagtt cctcgagcgc agctgcgtcg agcggcgccg
     7561 gacgcgggtt cgacattagc cacagacgcg cggtctcttc gagctcttcg atcgcatacg
     7621 aggcttgtga caccgaacgc tcccacacga ccggcccgag ccgctccagc atcacgccgc
     7681 gcacgctttc ggccagtgcc gcaatctgtt gcgcgacctg cgggtcccct ggacgccggt
     7741 aacggatcaa tggcacgtgc ccgaccttca tcacgaagta aggcgtgatc ggcggcagca
     7801 cgtcagcttc gctccagacg cccgcgagcg tcagcgcgac gaggtgggtc gaatgcgtgt
     7861 ggacgatgcc gcgcacgtcg cgattgcgcg catagacgcc acggtgcagc gcgagcgtct
     7921 tcgacggccg gtcgccggac accaggttcc cggccagatc aaccttggcg atccgggccg
     7981 ggtcgagccg gcccagacag gcgtcggttg gcgtaatcag ccagccgtcc tcgaggcgcg
     8041 cgctgatatt acctgcactg cccaccgtat agccgcgcgt gtacaagctc gcacctgtca
     8101 cgcagatttc ttcgcggatc ttcgcttctt cgctggcgtg aacggcgcat ggcacgggac
     8161 gggtcatcgc atgcctccgt cgagctggtg cagcgccttc gcgaagaagt cgacggtgcc
     8221 gaagttgccg gacttgagcg cgagcgcgag cggagattcg ccgattgtcg ccgtagcggg
     8281 cacgcccgga tcgatctgtc tgccgatgcg cagcatcttg acgccaagcg cctgaaccac
     8341 cgcgcccgat gtctctccgc ctgcgaccac gaatttgcgc acgcccagtt cgcgcaggcc
     8401 gcgcgcgatc gccgcgagcg tgcgttccac caggtgcccc gcctcggctg cacccaactc
     8461 gcgttgcgcg gccttgacgt cctgcggggc ggccgtggcg taaatcagca ccggctgcgg
     8521 gacgcgcgct tccatatgct cgcgcgcgaa cgccagtgcg cgctcgacga caggttcgcc
     8581 gcgtgcggca gcgagcggat caatgcggaa cgccggccgg ctctcgcacc aggcggccac
     8641 ctgcgcgttc gtcgccatcg aggcactgcc cgccagaaca gcagacctgc cttcgatgga
     8701 tggcagatcg ccagcagccg ccgcatcaac gagcaagccc gcgcggcggt aattttctgg
     8761 cagacccaac gcaatgccgg agcccccggt gatcaacgga acgtcggcgc aggcttcgcc
     8821 gagcgtgcga atggccgcat ccgaaaccgc atcggcgatc gcgatacgaa cgccttcgcg
     8881 ccgcaatgcg tcgatcgacg cgcgtacagc gtccacgcct tgcgcaacgg cgtcgtagcg
     8941 caccaggccg accttcgagc gcgtttggcg ttgcagcacg cggacgaggt tggcgtcggt
     9001 cattggcgtt agcgggtgat gctccatgcc cgattcgttc aacagcgaat cgccgacaaa
     9061 tagatgtccg cggtagaccg tgcggccatt ttccgggaat gccgggcagg cgatcgtgaa
     9121 cggggcgtcg tcgcctgccg acagggcgtc gagcaacgcg tccgcgacgg ggccgatgtt
     9181 gcccgcgtcg gtcgagtcga aggtcgagca gtacttgaag aagaactggc ggcagccctg
     9241 cgcacgcagc cagtctagcg cggcgagcgc ttgcgcgacg gccgtggccg tatcgaccgt
     9301 gcgcgacttg agcgcgacca cgagtgcatc cgcatcgatc ggatcggtgc tcgcaggtgc
     9361 gcctatggtc tggaaggtgc gcatgccgcc ttgcaccagc gtgcaggcga ggtcagtgcc
     9421 gccggtgaag tcgtcggcaa tgcatcccag caatggacgt ggggagtgag taagcatgga
     9481 aaagcctccg tgaacgacca ttttaacgcc acctgatcga gatgtgttaa catttgataa
     9541 tatactgtaa catcatggta cgtggcgtta acgcgctgcc gacctccttc cagatttatt
     9601 tctccgggca cggcgccgga aacctccgtg gattgaaaag acgggctccg cgtcacctgc
     9661 gggaaggacg ctgatcggcg ttctcgtcgg atagatagcg gcgcccacaa tttcaagaga
     9721 tgtccgtccc gcgtttcaga gaccgatcca tgtctagcca cgcccccccc gattcgagcg
     9781 aagcccctct aatacccgat cagcgacggg aagccatgct ccgtcagttg cggcgggaca
     9841 aggtcctgtc ggtccaacag ttgatggcgg cgttcaactg ctcgcacatg acggtccgac
     9901 gcgacatcgc ggcgctcgaa caggcgggcc tcgtttttac ggtgccgggt ggcgtacgca
     9961 tcgcgagtca tttgggcaac gagccgagtc accagtcgaa gttttccgtc gagcagccgc
    10021 agaaacaggc gattgcgcgc gtcgctgcgc aaagcctgcg ggccggcatg acgatctacc
    10081 tcgacgcggg cacgacgatg ctcgcgatcg tgtcgcacat cgtcgcgctc agcgacatga
    10141 cggtcatcac caacgacttc gagatcgtgc gcgagctgtc tggcgcaacg catgtcagcg
    10201 tgatccatac gggcggccaa ctcgatcacg cgaatctgtc gagcgtcggc ccgctcgccg
    10261 ccacgacgct gcgccaggtc gtactcgacg tggccttcat ttcggcgagt tcgtgggatc
    10321 tgcggcgtgg cgtgacgacg ccgtctgcgc caaaggtgga agtcaagctg gcggctatgg
    10381 aagccgcgtc gcgcacggtg ctggcagtat cgagctcgaa gtacggcacc tttggcctgt
    10441 acaaggtggc ggcgctcgac cgcttcgatc tcatcatcac cgacgacggt ctcgcgccgg
    10501 ccgcggtgct cggcatgcgg cagtcaggcg tcgagctcga gctggcgtcg gtcggttgag
    10561 tcgaggcgac gcaaccaccg cggcgcaaga tccatctgtc gatcgacggg gagatgaaca
    10621 ttcacgcgat cagcattacc acgacagaag tatcggacag cgaaggaatg gacacggtgc
    10681 tgcccgcgga tctgccagtc gaccgggtga tcgcggacgg cgcgtactac agcatcgagc
    10741 gcaccgaagc gctctcaagc gcgggcgtga cgcctgtcat tccaccgtcg gctcacgtcg
    10801 tcgtgcacgg caacgaccag acccgctggc atgatcagat cgtcaaatac atcgatgaga
    10861 aaggcatcta tgggttcctc gcccgctgca tgggaactca tgcccgtccg aacaaagtcg
    10921 ctcctgagcg ctgtgtatgg ggggctgact ggccgcatcc ggatcttctt aacggaatgc
    10981 cccgatgacc gtgcaatgtt cgacctactt accgcgtggg taccgacgac agcaatgcgc
    11041 aaccagattc ttgtgcacaa tccagcagaa ctctacggat ttgctgatta agatttagaa
    11101 atggctgcaa ctattgtccg gctgaaccgg ctgtcccaac cccgctctgc tatctactga
    11161 ctttcgcgtg ctcggagcaa gcaatatgcg cgaactcctc aaccagctgc gaacgaaccg
    11221 atgctcgcag ccatcctaat ccgacgcgtc gcgtcgggca cggctgcggc aggctccagc
    11281 gtcgaagctg cgggtcgggc acgccgatta ctggccagtc gggaagcact gacacgccga
    11341 agccttcggc taccagcttc gcaatgtgct ctatcccgtc cagctcaaac tgcacgtttg
    11401 ggcgaacacc ctttgcgtgc agatattcgt cagccatctt tcctgcgaca actctccggt
    11461 cgtaacgaat aaacggctct tcggccacgg tggctaacgc gtccttgaca cgcagacgtg
    11521 tctgggtaag aaggatgaga ggctcttcgc gcaacaggcg ccacgagcaa gtcttcggaa
    11581 gctcaaaagt cggatgcacg atgatggctg catccagttc tcccgacatt acgccgtcta
    11641 gcaacgtgac tgaggtccca ggctcgatgt aaatgggaat gcccggatgc gtttgtttcc
    11701 agcgccgtag aaatggcggc agaagtccca tcaatgccgt tggggttgcg ccgagacgca
    11761 acgggccggc ggggaggccc gtgtccgagg ctgcggagcg taggtcgcga acgtctcgaa
    11821 ggaggtctcg cgttcggtcg agaattctgg cacccactac tgttggctta accgtccgtc
    11881 ctgcgcgcgt gagcaacttg gaaccaatat cattctccag cgcgcgtatt tgctgcgcga
    11941 ctgtggtcgg ggcaaggtcg agccgtcgcg ccgcctccgc aattgagccg gattcgataa
    12001 cagcaatgaa gctctgaaga aaacgggtgt ccatacgcgt attttacgtg ttctaaagat
    12061 gtcagagacc gcttttttcg ctgttaccta acatgcaaac tgtctccgtg cttgataacc
    12121 aacccgcatt cgaaggagac gtgccgtgcc catcatcaaa tctatcgaag tctgtgctgc
    12181 tcgtgttccg ctggacaagg tcacttcttt ctccaaccgg actgtcaccg agcgccacta
    12241 cggattagtt aaagtccgta ccgcagacgg tatcgaaggc gtcggcttct gctacgtcgg
    12301 aagctctgcg ggggacctct tccgggttgc agtggaacaa ttgctcgcgc cggtgattct
    12361 cggtcgcgat tcgctcgctg tagagggcct gtggaaagac atgtaccaag agtctctgct
    12421 ccaggggcgc atgggtacgg taatgcgcgc gctaagcgcc atcgacattg cactttggga
    12481 catcaactcc aagagcgcag gcttgcccct ccataagtac ctcggcgccg tggatttgga
    12541 cacggtggcg gcctacgcga gcggcggcta ttacgttgaa ggtaaaaccg ctgaaatggt
    12601 cggcgaagag atggccagct ttgtcgccaa gggcttcaag gccgtgaaga tgaaaacggg
    12661 tcgttggtcg cctctaggcg aggaggctcg ggtcaaggca gcccgcgaag cgattggtcc
    12721 agacgtggag ttgatgctcg actgtaataa cggttggatc gataccgttc aagccatgca
    12781 gtacttgagc cgcattgaac agtacaaccc ctactttatc gaagagccgt tcagccccga
    12841 tgacatcgag agccacgcac gcctggcgaa gctcacgcgc atcccggtgg ccaccgcaga
    12901 gattggttat ggccgctggt accataagca gctactcgat atgggcggcg ctgcaatcct
    12961 ccaaaccgac gcagcagtct gcggcggcat tacggaatgg aagcgtatcg ctgcgctgtc
    13021 tgcaggctac ggtgtgcagg tatgcccgca ttggttccac gacgtccatg cgccactggt
    13081 tgcagccacg ccgaacgcac gctacgtcga attcttctgg gacgaccagg tcctcaactt
    13141 ccgtcagctc atcgaccggc agctggagca acgtgaaggt cgagtcgttc tgcatcaaac
    13201 gccggggctg ggcttcggct tcgacgagaa gcaagtcgcc aagtacggcc gttggactat
    13261 cgtttcctaa gactttgaca tcctgcgcaa attgacagtc gctggtgctt gctttcggcg
    13321 aagcaccagc gggaagtcac gtgcgcgttg tcgacgagct tctctccatt ttcaagcatc
    13381 attagctcgc acacgcgttg ctacggcggg cgaatacgtt acagcaaaga agaactgcca
    13441 gtgtgatggc gttcgagaga gttgctggtc aggttgagca agaaaccgcc tcagagtcgg
    13501 tatcagacta tttgtcagga gacgatatgc agcaatccaa cgaatattta atcgtgcgca
    13561 aaatattttg gcgcgtgatt ccgcttctca tggtccttta tgttgtttca tacattgacc
    13621 gcattaatgt tgggttcgcg gcgctgacga tgaatcacga catcggactc aacgagtaca
    13681 catacggttg gggcgcaggc atttttttct tcggttactg tctctttgaa gttccaagca
    13741 acctgatgat ggtgcgattt ggcgctcggc gatggatagc gcgcatcctc ttcacctggg
    13801 gtcttctctc gtgcgcaatg gccttagtcg ctggaccgac gagcttccta gtacttcgat
    13861 ttcttcttgg cgttgcagag gcggggtttt ttccgtgcgt tatcttgtat ttgacttttt
    13921 ggttccctgc acggtaccgc gctcggatca tcgctacatt cagcctcgcg attcccgtgt
    13981 cgattgctgt cggcgctccc ttgtccacgc tgatactgga acttgacggt atgctcggag
    14041 tgaaaggatg gcaatggctt ttcattattg aaggcatccc tgctgtactc ggcacgttct
    14101 tcgtattgcg catgctgacc gaccgcccag aagaggctca atggctgacc acacaggaga
    14161 aagcgtggct cgctgacgag ctcaataagg acattgtggc tagcaacgaa cacaatgttt
    14221 ctgcgttgcg cagggcgttc accgatccgg ccgtgctcac actagcgtta gtgtatttct
    14281 gcgcaatcgg tgcaaatctt ggactgtcct tcttcttgcc gcagattatc aaggcgcaag
    14341 gttatagcac gatgcaaacc ggcctcatta cgtcggtgcc gtacatcgct ggctgcgtgg
    14401 gaatgctcgc cattggcttt ctgtccgacc gtttcaatga gcgccgctgg ttcttggccg
    14461 ccaccatggt gctgacggcc ggaggcctcg cagttgcagg agcattgggc gcctctgttt
    14521 ggtccattgc ggcggtgagc gttgcagcaa tcggcattct tggttgcaag ggccccttct
    14581 gggcgctacc tgcaacctac ctcagcggac aggcgatggc cggcggcatt gcgttcatca
    14641 acgcgatggg aaatcttgga ggtttcgccg gcccgtatct ggtgggattc ttcaagagtt
    14701 cgtcgaacag ctatgcgagc gggctctatg cgttagcggc cctagtacaa cgtcccgcaa
    14761 ttacttttaa taaatgtttg catgatctat cctggtggca tgagccgagg tcgtcatgca
    14821 acgccagtga agctggtcag gaaggaccgg gaggaactgc tggcgctgat caggcgcacg
    14881 acggcagcac agcgtgatgt gacgcgggca cgcatcgcat taatggccca tgagggctac
    14941 tcaggcgcag cgatcgcgca ggaacttggc gtgtcggtgc agacggtcag cctgtggcgc
    15001 cagcgcgttg cacggcaagg cgctcgaggt atccgggagg gtgagcgtag cggtcgtccg
    15061 ccgcgcatca cgcaggaagc gcgactgcaa ctgatcgcgc tggcgtgcga ggtgcaggac
    15121 gcgcacggcc gggttacgcc gacgctcgat gagatcatgg cgcgcgccgt cgagcgtggg
    15181 atcgtcgcac agatcagccg cagccatgtg caacgcatcc tgcaggccgg cgacgttcgt
    15241 ccgcaccggg tgcaacagtg tctgcacagt ccggatccgg cgtttcgcga gaaggtcaac
    15301 gtgatttgca ggctgtatcg caaggcgccg aagaatgcgg tcgtgctgag cattgacgag
    15361 aagaccggcg tccaggccat tgaacgtaag catcctggac gcgcgcccgc agcgggacgg
    15421 ttgcgtcgtc gtgaatttga atatatccgt catggcacgc aggcgctgat tgccgcgctg
    15481 gatgtgcata ccggccaggt gctgggcagt tgccgcgacc ggcgcaccca ggacgacctg
    15541 gtggccttca tggacagcgt ggcggccgcg tatccgggca aacaggtgca tgtgatctgg
    15601 gacaacctga acacgcatcg tgcacacgcc gtctggcagg cgttcaacgc gcggcacagc
    15661 gagcggtttc acttccactt cacccctttg cacgcgagct gggtgaacca gatcgaactg
    15721 tggtttgcgg cctatacgcg ccgtgtgctg cgtcacgcca gtcacctcag cactgcgcat
    15781 ctgcgcgagc gtacagcgca tttcatccgc cagcgcaatc agacggcgcg ccccttcaga
    15841 tggactttcc ggggctaccc gttgcaaact ggcgcatcct gaaccggagc gctcatgcct
    15901 gccttacccg cgaaacatca tgcaagcgaa ctgcagcgtc aactgcgcgc cctgctgggt
    15961 cacgaccaga tcgtcacaca ggcttacggg cgtcatctgc tgatcaaacg cctggacgat
    16021 caggagccga ccgttgtggc acgcctgagt gaactcagcc gcaatcgtta tggtgcggcc
    16081 tttcgtagcc atagcgggcg atgggaacct ctgcctggct caggttcgct cgacgagatc
    16141 gctcaggtag tcgtcacgct cctgcagccc tatttgcagc cagacaatta ttaaacctat
    16201 ttgagggatg ttgtactagg agcagcggct gccgtaacta ccgcgttttt gcgaagtttt
    16261 cataacgtgt ctccttgtgt ttggtctgaa gccgcccttg cgctcgtgag aagaaccggc
    16321 atctcaccgc cttctatccg ctggcgtgtc accagcctta tcctgcatgc gcgttacgcc
    16381 ggaatcaatc ccgggaacat gccccatctg aaagtattcg gttgcttgag caacccagtg
    16441 tgatcacggg catgttcact ccaaccgtgt ttgctgcgta actgcctagg tgaaccttta
    16501 cctccttcct gtgaaaccag agggactcaa gctgacttaa ggtgcgcgcc ttcgggaatg
    16561 accccgtctt tcagatagcg ccacagcgca atggcaagac gtcgcgcgac cgcgacgatg
    16621 gcgatccgtc gcacacgtcg gttcggtccc gtaccgtgtg tgcgctgatc aaaccatttc
    16681 gctaatgcgc tgccgggctg gtagcgaagc caggtccacg ccatctcgat cagcagcgcg
    16741 cgcacccgcc ggtttccctg tttgctgatg ccctggtcga cctgactttc gccactgtcg
    16801 tagggctgcg gcaccagccc tacgcacgca ccgacttcgc ggcggttgtt gaagtcgcgc
    16861 cagaacaact cgagggcaag gcgcgaagcg cctacttcgc caatcccctt caggcgcgcc
    16921 agcgcatcgc aacgctggcg cgccggtgct ggtacgtttg tctgtcgtgt cttctccagc
    16981 gcggcaagtt gctgttcgac gagtgccagc cgttcgcact cgcgcagtaa ccgctcacgc
    17041 atctcctgcg gtaacggcgc accgtcgtgg cagcgcacct cttcgcgctc gagccggtcg
    17101 gcgaaggccc ggtgatcgac cgcatcccag cagccaagcg tggccagcag cttgcgcatc
    17161 cggtcgcggt gctgcaggac ttccttctgc agttgcccgc gttcgcgcat caggtggcgc
    17221 gatgcctcgt cctccggtga cggcgcatgc accacgtgca tgcggtcgcg ctcgccgcgc
    17281 agccacgcgc gcaggttgat cacaagtttg atgacatcga gccggtcggt cttcgcgcgc
    17341 cgcctgtgac gttcgacggg gatgctggcc ggatcgacga cgtagcactc gatgccgtgc
    17401 gcctgcagcg cacgacagat ccagaatgcg tcctgtccgg cctcgtagct cacgacgatg
    17461 cgcacgccgg ccggcagtga ccacttctcc ttgtccgttt cgatcaggtc cagcaccgcc
    17521 tgcagacgcg ccgcggcctg cggctgtgcg accgtgtgca cggccggctt ctcgcgccga
    17581 ccatcgtgaa gcgcaacttt ccatttggcc gccgccagtt ccagcgacac tgccagcact
    17641 ggtacgtcac cattacctgt atatgcctga tcg
//
LOCUS       DATASH010001054         1704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2359821,
            whole genome shotgun sequence.
ACCESSION   DATASH010001054 DATASH010000000
VERSION     DATASH010001054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2359821"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /gene="paaC"
                     /locus_tag="VHV99_27995"
     CDS             <1..739
                     /gene="paaC"
                     /locus_tag="VHV99_27995"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HEX3639255.1"
                     /translation="QRNAEWCGHGPILEEDIALSNMSLDLIGQARLLYTHAASLEQQL
                     TGKSRTEDDYAYFRAEREFANYTLAELPHYGPLAGTAQAEKDYAVTIVRNFLYSTLMA
                     HVWTALTASKDEQLAAIASKSIKETSYHVHHAREWLIRFGDGTDESHRRAQAALDYLL
                     PYTREFFSADAVEEAIAAAGIGPLTSDLEAAWLEDVRATLDEATLNLPEAVKHVTTGK
                     HGEHSEHMGFVLAEMQSLARQHPGATW"
     gene            771..1316
                     /gene="paaD"
                     /locus_tag="VHV99_28000"
     CDS             771..1316
                     /gene="paaD"
                     /locus_tag="VHV99_28000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HEX3639256.1"
                     /translation="MTTSTATPTADAALERAWAVLEAVPDPEIPVVSIRELGILRDVR
                     RAADGALEVVITPTYSGCPAMSQIAEDVAHALDVAELKPYRIATVLAPAWTTDWMTAE
                     AREKLRAYGIAPPTGNCGSAAPSSQEKVMRFVPRALPAPSCPRCGSAHTERLAQFGST
                     ACKALYRCLDCREPFDYFKPY"
     gene            1318..>1704
                     /locus_tag="VHV99_28005"
     CDS             1318..>1704
                     /locus_tag="VHV99_28005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019255661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX3639257.1"
                     /translation="MATPQFHPLRIREVRPETADAVSVAFEVPVELRDHYRFTQGQFV
                     TLKTHIDGEETRRSYSICVGVTDYDRDGELRIGIKRVRGGRFSNFAFDTLQPGHTIDV
                     MTPDGRFFTHLNADQGQQYIAFSGGSG"
BASE COUNT          323 a          591 c          508 g          282 t
ORIGIN      
        1 tcagcgcaat gccgagtggt gcggccacgg cccaattctc gaagaggaca tcgcgctgtc
       61 gaatatgagt ctcgacctga tcggccaggc gcgcctgctg tacacgcacg cagcctcgct
      121 ggaacagcag ctcaccggca aaagccgcac ggaagacgac tacgcgtatt ttcgcgccga
      181 acgcgagttc gccaactaca cgctggccga gttgccgcat tacggcccgc tcgccggcac
      241 cgcgcaagcc gagaaggact acgcggtcac gatcgtgcgc aatttcctct actcgacgct
      301 gatggcgcat gtgtggaccg cgctcactgc gtccaaagac gagcagctcg ccgcgatcgc
      361 ctcgaaatcg atcaaggaaa ccagctatca cgtgcaccac gcgcgggaat ggctcatccg
      421 tttcggtgac ggcacggacg agtcgcatcg ccgcgcccaa gccgcgctcg actatctgct
      481 gccgtacacg cgtgagtttt tcagcgcgga tgccgtagaa gaagccatcg ccgccgccgg
      541 catcggaccg ctaacgtcgg acctcgaagc tgcatggctc gaagacgtcc gggccacgct
      601 cgacgaagcc acactcaacc tgccggaagc ggtcaagcac gtcacgaccg gcaagcatgg
      661 cgagcactcg gaacacatgg gcttcgtgct ggctgaaatg caaagcctcg cacgccagca
      721 tcccggcgcc acctggtaag cgcttcagcg caacccaacg gaactcgacg atgacgacct
      781 cgactgccac gcccaccgcc gacgccgcgc tcgaacgcgc atgggcggtg ctcgaagcgg
      841 tgcccgatcc ggagatcccg gtggtgtcga ttcgtgaact cggcattttg cgcgacgtgc
      901 gccgtgccgc cgacggcgcg ctcgaagtcg tgatcacgcc gacctattcc ggttgcccgg
      961 cgatgtcgca gatcgccgaa gacgttgccc atgcgctcga tgtggctgag ctaaagccct
     1021 atcggatcgc cacggtgctc gcgccggcct ggaccaccga ctggatgact gccgaagccc
     1081 gcgagaaact gcgcgcctac ggcattgcgc cgcccacggg caattgcggc agcgcggcgc
     1141 cttcgtcgca ggaaaaagtg atgcgcttcg tgccgcgcgc gctgccggcg ccgtcgtgcc
     1201 cccgttgcgg ctcagcgcat accgagcgcc tcgcgcaatt cggctcgacg gcctgcaagg
     1261 cgctgtaccg ctgtctcgac tgccgcgaac ccttcgacta cttcaaacca tactgatatg
     1321 gctaccccgc aatttcatcc gctgcgtatc cgcgaagtgc ggcccgaaac cgccgacgcg
     1381 gtctcggtcg ccttcgaagt ccctgtcgaa ctgcgcgatc actatcgctt cacgcagggc
     1441 cagttcgtca cgctgaagac gcacatcgac ggtgaggaaa cgcgccgttc gtattcgatt
     1501 tgcgtcggtg tcaccgacta cgaccgcgac ggcgagctgc ggatcggcat caagcgcgtg
     1561 cgcggcgggc gtttctcaaa cttcgcattc gacacgctgc agcccggcca caccatcgac
     1621 gtgatgacgc cggacggccg cttcttcacc catctgaacg ccgaccaagg ccagcagtac
     1681 attgcgtttt ccggtggttc gggc
//
LOCUS       DATASH010001055         4238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6709579,
            whole genome shotgun sequence.
ACCESSION   DATASH010001055 DATASH010000000
VERSION     DATASH010001055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6709579"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(578..1339)
                     /locus_tag="VHV99_28010"
     CDS             complement(578..1339)
                     /locus_tag="VHV99_28010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4410 domain-containing protein"
                     /protein_id="HEX3639258.1"
                     /translation="MQTSFNSLVRRAKRLAGTAVLAGSLLLSGCAFGATVTTQADAAL
                     HVRADVIYVYSFDATPDQVKMDSGMAQELKTAMSGESSAQKQYQTALDTREQVADEIV
                     RELQSMGLRAVRVDGPAPANQNALIVEGNFQTIDEGKRRRRMLIGLGAGKSEVGASVQ
                     ILYKPANGAPVPLQSFSANADSGHMPGIAETAGVGAAAGHVATAAATGASLHGVSEVK
                     RDSVAGDAKKLGDSIAKQIAAASVANGWMSAERME"
     gene            1719..2651
                     /locus_tag="VHV99_28015"
     CDS             1719..2651
                     /locus_tag="VHV99_28015"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011354517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX3639259.1"
                     /translation="MSTTEKIALCVTLFVVGVSLIEACVLTRKQRDSATPFDWYEVWI
                     SLADVAGRKLLALLPLSLATPIFALAWDHRIFTVTLNSALMVFLLFIGQEFCYYWYHR
                     ASHRIRFFWATHAVHHSPNQLTLSSAYRLGLTGKLTGSAMFFAPLVWLGVRPEIVLAT
                     LSFNLLYQFWLHNTWTPKLGWLEYVFNTPSAHRVHHASNVDYLDANYGGVLVIFDRLF
                     GTYVEERADEPCRYGLTTPTRSRNPLVVEFEHWVSLARDIASAGSVWTAISYVILPPG
                     WRADGAGETTEALRQQNLAARRAVPVSTQPHLKQ"
     gene            2958..3905
                     /locus_tag="VHV99_28020"
     CDS             2958..3905
                     /locus_tag="VHV99_28020"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HEX3639260.1"
                     /translation="MTRALYPTSAILKALAAAAVIGLASASVQADVPDPTTQITQSGY
                     SPVYVGVDAETATVPSNPATTGGKPTVSRAYVMRIDLRAPGVSVETTGQSGPLMTTSE
                     TISQFAARTNTRLAINANFFAPCCATTPEPKTLIGLLVSHGQVVSPLTTDPTQSEAVL
                     AITRQGRAYIAEAPQISAQDLKLIDTAVSGSAILLKNGQDLSAQSPNEGDPLDPNPRT
                     LVGLSHQGRFLYFVVIDGRVAGYSTGTTNAQSAQLMKAIGADDAINLDGGGSTELVRA
                     DQIGVPFIVDTPSGGAERLDASAFGVHALKLPEVPGLPF"
BASE COUNT          795 a         1359 c         1240 g          844 t
ORIGIN      
        1 tgctgaagcc gatgcgtcgc gcctacgcgg aacagcacgc tgcgccggct gtggagatgc
       61 cgtatggtgc atcgttcgtc agtgactcgg ctcgagtgtc ggccgacggc ggttagtcgg
      121 cgaggcaatt gagcgttccc gatcgactgt gagcgcgacg acgccgggca gtcccttcct
      181 ggccggtcac tgcctgacgc aatcagtgtc acccggcccg caagagcgcg ttttcaatct
      241 ctcaaaggga gtcgttcagc tgcatcagct agttcttcga atcgcgccgt ttgctgtccg
      301 ggcggcccaa aacgccgccc gtcagcaagc ggaaaagacc actctcgcga cgccttcaaa
      361 gttgggctag ctggaccaaa aaggcgtcaa cctgatgttc ttgagtggcc gatgacgtta
      421 ccaggcgaat cacagaatga tcgaagacta gccccgaaag ttgcgctgtg aggtgttcat
      481 tatcccagct tccttttgat ctgcactgac tcccccacgt caccccctgc gactcgaatg
      541 ctttcaggag gtccagcgat gccttcacgc gcacccctca ttccatacgt tcagccgaca
      601 tccagccgtt cgcgacgctt gccgccgcaa tctgcttcgc gatcgaatcg cccagctttt
      661 tcgcgtcacc ggccacgcta tcccgcttca cctcggacac gccatgcagc gacgcgcccg
      721 ttgccgcagc cgttgcaaca tggccggcag ccgcgccgac acccgctgtt tccgcgatgc
      781 ccggcatgtg gccactatcg gcgttagcag agaagctttg cagcggcacc ggtgcgccgt
      841 tagcgggctt gtacaggatc tggaccgatg caccgacttc gctcttgcca gcgccaagac
      901 cgatcaacat gcgacggcga cgcttgcctt cgtcgatcgt ctggaagttg ccctcgacga
      961 tcagcgcgtt ctggttcgcc ggtgcagggc catccacgcg caccgcgcgc agtcccatcg
     1021 attgcagttc gcgcacgatt tcgtcggcga cctgttcacg tgtatcaagc gccgtctgat
     1081 actgcttttg cgcagacgat tcgccgctca tcgccgtctt cagttcctgc gccatgccgc
     1141 tgtccatttt cacctggtcg ggcgttgcat cgaacgaata cacgtagatg acgtcggcgc
     1201 gaacgtgcag cgcagcgtcg gcctgggttg tgacggtggc gccgaaggcg cagccgctca
     1261 gaagcagcga tccggccagg acagccgtgc ctgccaggcg ttttgcccgg cgaacgaggg
     1321 aattgaatga ggtttgcatg tcgatcctgt catcggcaca ggcgtgccgc aaattgtcga
     1381 gggagcgtca ccgacaatcg ttcgatccac aggatcacaa actatcggag atcacgtctg
     1441 cgtgagtgcg cgctgtctgt gagagaactg tgccgcaacc cgcagacgca agtatgggag
     1501 gaagcggttc tgcgagctat ccgcgaatta tgtcgcgcca tgtcgccggt ctgccagcgc
     1561 acctgaatgc ccggcggcgg ccggaattcc gcgacatccg gcgacatagt ttccgcatgg
     1621 cttttaacct ggcagcccac tacattagct tcacccgacg cgagagcggc cttgtcccgc
     1681 agatgacgcg tcccatctca acccgttacc tgctcatcat gtcgaccact gaaaaaatcg
     1741 cgctgtgtgt tacgttgttc gtcgttggcg tctcgctgat cgaggcgtgc gtgctcaccc
     1801 gcaagcagcg cgacagcgcc acgcccttcg actggtatga agtatggatt tcactggccg
     1861 atgtggccgg ccgcaagctg cttgcactgc tgcccctttc gctcgcgacg ccgattttcg
     1921 cgctcgcctg ggaccaccgg atctttaccg tcacgctcaa tagcgcgctg atggtgttcc
     1981 tgctctttat cggacaggag ttctgctact actggtacca ccgcgcgtcg caccgcatcc
     2041 gctttttctg ggcgacgcat gcggttcacc actcgcccaa ccagctgacg ctttcgtctg
     2101 cctaccggct tggcctgacg ggcaagctga ccggttcagc catgtttttc gcgcccctcg
     2161 tgtggcttgg cgtgcgtccg gaaatcgtgc tggcaacgct gagtttcaac ctgctttatc
     2221 agttctggct gcacaacacg tggacgccga aactcggttg gctcgaatac gtgttcaata
     2281 cgccttccgc gcatcgcgtg caccacgcgt cgaacgtcga ttatctcgat gcgaactatg
     2341 gtggcgtgct cgtcatattc gaccgcctgt tcggcacgta tgtcgaagaa cgcgctgatg
     2401 aaccgtgccg ctacggtctc acgacgccga cgcgctctcg caatccgctc gtcgtcgaat
     2461 tcgaacactg ggtcagcctc gcgcgcgata ttgcatcggc gggcagcgtg tggactgcga
     2521 tcagctacgt gatcctgcct ccgggctggc gtgccgacgg tgccggcgaa acaaccgagg
     2581 cgctgcgtca acagaatctg gccgcgcgtc gcgcggttcc agtcagtacg cagccgcatc
     2641 tgaagcagtg agcgggcctg gcgacgccga gggacgaacc tcggcgtcac ccgacgacgc
     2701 gacggaaaaa agcaaaccgc catgttgttt ggcgggcaac gcacagacac taatgggcta
     2761 acaggtcatt cgatgaaaag gtcgaagaac cgcacagaat ttctttcgac atttattttt
     2821 ctgtcgacgc acccattgcg catcatcgaa agcagaacga aaagaaaaaa taacatggtg
     2881 gtcatgtcgc gtctctaagg tcagcggcac ggtgaaaggt gacgatctcg tcgccgccgt
     2941 caatggagat taaacagatg acgcgtgccc tttaccccac ctcggcaatt ctgaaggcgc
     3001 tcgccgccgc ggcggtcatc ggcctcgctt cggcgagtgt gcaagccgac gtgcccgatc
     3061 ccactaccca gatcacgcaa agcggctact cgcccgtgta tgtcggtgtc gatgccgaaa
     3121 cggcgaccgt accgtcgaac cccgcgacca ccggcggcaa accgaccgtg agccgtgctt
     3181 atgtgatgcg catcgatctg cgcgcgccgg gcgtgagcgt cgagacaacc ggccaaagcg
     3241 gtccgctgat gacaacctcg gagacgattt cgcaattcgc cgcgcgcacc aacacgcggc
     3301 tcgcgatcaa cgccaatttc ttcgcgccgt gctgcgcgac gacgcccgag ccgaagacgc
     3361 tgatcggctt gctggtgtcg cacggccagg ttgtcagccc gctgaccact gacccgacgc
     3421 aaagcgaagc cgtgctcgcg atcacacggc agggccgcgc ctatatcgcc gaagcgccgc
     3481 agatttcggc gcaggatctg aagctgatcg atacggcggt ctcgggttcg gccattctgc
     3541 tgaaaaacgg ccaggacctc agcgcgcaaa gcccgaacga aggcgatccg ctcgatccga
     3601 atccgcgcac gctggtcggc ttgtcgcatc agggccgctt tctctacttc gtcgtgatcg
     3661 atgggcgcgt cgcgggttat tcgacgggca cgaccaacgc gcaatcggcg caactgatga
     3721 aggcaatcgg cgcggatgat gcgatcaatc tcgacggcgg cggctcgacc gaactggtgc
     3781 gcgccgatca gatcggcgtg ccgtttatcg tcgatacgcc gagtggcggc gcggaacggc
     3841 tcgacgcgtc ggcgttcggc gtgcatgcgt tgaagctgcc ggaagtgccg gggttgccgt
     3901 tctaaggtgt ataacgtctg aaattgtgcc ggttccttca aaatggccgg gcgcttacgc
     3961 gtccggtttt tttctggcat gcacacaaaa atctgctaat tttcaggcac gacacgcttg
     4021 ggcatcagga ttgctgctca atcgaccggg cgcgcgtgac gcgtcttcag atactcgccg
     4081 gcctaatgcc ctgcgacccg gcgagcggtc gccaatggat ccagttcctg cttcagtctg
     4141 ccgcccggcc gcgccgcttg tggccgctct cgccatgagg agaacgcatg atgcaacaga
     4201 ccgcctcccc caccacgcaa cagagcgccg accacgac
//
LOCUS       DATASH010001056         3753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_378159,
            whole genome shotgun sequence.
ACCESSION   DATASH010001056 DATASH010000000
VERSION     DATASH010001056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378159"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..2729
                     /locus_tag="VHV99_28025"
     CDS             105..2729
                     /locus_tag="VHV99_28025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3639261.1"
                     /translation="MKLESIAIQEFKQFTGRLVIDDLQPGLNLFTGPNEAGKSTIAEA
                     VRAVFLERYKASHLKDLLPWGKASGQPSVEVTFDMDGTACRLSKQFVTRQRCELKIGQ
                     AVFGEDEAEDKLAALLGFSRAARGPLKAENAGVPGLLWVQQGGTQEVRDSTGHAAQYL
                     RDALSQLSGGHESAGEDALIAAVQRELRQLLTARTQKSTGPLAEAEQRLAALIVERDD
                     LEQQRQQFDENIARLATQQEAFEDAQRKRPWALHEQKAVQAQQRADAAAELERSLQGL
                     AQSLKLSDAELALSVQQEQAATELEAAVARERQQLDAARANVSAVQNEHAQAQASVTQ
                     FEQVHADASRAQELANAAVTAGDLRSQIALHRTERERLEASIEAAGKANEAVLEATRA
                     AAALEIDGMQLKRLQSLDAGLTVLRARTDAAMTRIEYRLNGAVTVGDIQVSGEGVLRL
                     DEEKRIGLGALGELRVIPGVSDLSAQLSELASLEARHAQLLQALGVASLGEAEARQEQ
                     WKALVAQQKSQAKILEVHAPKGIDALRAALASVDARLQSANGRLAALPDVSSAPPLDE
                     ARRNADIARDALDAARAVLAQAAEKRSTATATTESLAVQLQRKEAHLSDEAFCRNRAQ
                     WQTKIVEQRVQVEALKKQLEGRERELEAARLDDPAAEAKRYRASAELARNEQHERQVR
                     IAQLRSQLETVGASGLGERLAAVKAAVEQATRRKDELSLRASALSLLDEVLVDERDAA
                     VAQLRAPLTERLGHYLKRIFPQSTIALGDDLSPATLDRYGRADTLDALSFGTREQLGI
                     LTRLAYADLLKASGRPTLLMLDDAAVHTDAARRDAIKRALIDAATRHQILVFTCHPEL
                     WDDLGVRQRAIEDLKVAA"
     gene            complement(2758..>3753)
                     /locus_tag="VHV99_28030"
     CDS             complement(2758..>3753)
                     /locus_tag="VHV99_28030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639262.1"
                     /translation="NAAGHETEYATFNANGTKTQDLFYNATTGQLTQENDFNADGSQI
                     DRVYNTNGTQTATVFNAGGHETEQATFDASGKISKDLVFDGKTGAERQETDYNANGSG
                     VAHIFNPDGTQNAAVFDPSGHLSEYATYGTNGKLATDIFYDANGRATQYNEYTSNQTT
                     IHQFNADNTQTATVYNSAGQETEFARFDTSGHKTDDYFFNGSTGRETQYDSYGSDGSM
                     VEHLFNSDNSQDAIIYDASGRETQYDAFNSQGQLTGFTDFTYTVGGGYDALAYGPTGF
                     ETGWSNYNGDGSLISSSGGQYNFSLDDGYGDGTGDWDFSAPVYDDGYASDFGFYY"
BASE COUNT          661 a         1063 c         1325 g          704 t
ORIGIN      
        1 caggaggacc cgcagtccga cccgcaacag gtcaaacgcg cggcggaggc gttattgctg
       61 ctggcgcgtt ttcaacgtga actgccgcgc gaagcgaggt ccgcatgaag ctggaaagta
      121 tcgcgattca ggagttcaag cagttcaccg gccggctcgt tatcgacgat ctgcagcccg
      181 ggctcaatct gtttaccggc ccgaacgagg ctggcaagag cacgatcgcc gaagccgtgc
      241 gggcggtgtt tctcgaacgc tacaaggctt cgcatctgaa agacctgctg ccgtggggca
      301 aggcgagcgg acagccgtcg gttgaagtga cattcgatat ggacggcacg gcgtgccggc
      361 tgtcgaagca gttcgtcacg cgccagcgct gcgagctgaa gatcggccag gcggtgttcg
      421 gcgaggatga agcggaagac aagctcgctg cgttgctggg tttctcgcgt gccgcgcgtg
      481 ggccgttgaa ggcggaaaac gcgggcgttc cgggtttgtt gtgggtgcaa caaggtggca
      541 cgcaggaagt gcgcgattcc accgggcacg cggcgcagta tctgcgcgat gcattgtcgc
      601 aactgtcggg cggccatgaa tcggcgggtg aggatgcgct gatcgcggcg gtgcagcgtg
      661 agttgcggca attgctcacg gcgcgtacgc agaaatccac cggaccgttg gcagaggccg
      721 agcagcgcct ggcggcgctg attgtggaac gcgacgatct ggagcagcag cgtcagcagt
      781 tcgacgagaa tatcgcgcgc cttgccaccc agcaggaagc tttcgaggac gcgcaacgca
      841 agcgcccctg ggcgcttcac gagcagaagg ccgtgcaggc gcaacaacgc gccgatgccg
      901 cggcggaact cgagcgcagt cttcaggggc tggcgcagtc gttgaaattg agcgatgcgg
      961 aactggcgct ctcggtgcag caggaacagg ccgcaacgga gctggaagcg gccgtcgctc
     1021 gcgagcggca gcaactcgat gccgcgcgag ccaatgtgtc ggcggttcag aacgaacatg
     1081 cgcaggcgca ggcgagcgtg acgcaattcg aacaggtgca cgccgatgcc agccgcgcgc
     1141 aggaactggc gaacgctgct gttaccgccg gcgatttgcg gagtcagatt gcgcttcatc
     1201 gcaccgagcg cgagcggctg gaagcgtcga tcgaggccgc cggcaaggcg aacgaggccg
     1261 tgctggaagc gacgcgcgcg gcggccgcgc ttgaaatcga cgggatgcaa ttgaagcggc
     1321 tccaatcgct cgatgccggg ttgaccgtgc tgcgcgcgcg gaccgacgcg gcgatgacgc
     1381 gcatcgagta ccgcttgaat ggcgcggtta ctgtcggcga tatccaggtg agcggcgagg
     1441 gcgtgctgcg gctcgacgag gagaaacgga tcggtctggg cgcgctcggt gaattgcggg
     1501 tgattccggg cgtgtccgat ctgtccgcgc agctgtctga actggcgtcg ctcgaagccc
     1561 ggcatgcgca gttgttgcag gcgctgggcg ttgcttcact gggcgaggcc gaggccaggc
     1621 aggagcagtg gaaggcgcta gtcgcgcagc agaaaagtca ggcgaagatc cttgaagtgc
     1681 atgcgcccaa gggtatcgac gcgctgcggg cggcgttggc ttcggtggat gcacgattgc
     1741 aatcggctaa tggacggctt gcggccttgc ccgatgtgtc ctccgcgccc ccgctcgacg
     1801 aggcgcgccg caacgccgac atcgcgcgtg atgcgctgga cgccgccagg gcagtcctgg
     1861 cgcaagccgc cgagaagcgc tcgaccgcaa cggccacgac ggaatcgctc gccgtccagt
     1921 tgcagcgcaa ggaagcgcac ttaagcgacg aagcgttctg ccgcaaccgc gcacagtggc
     1981 agacgaaaat cgtcgagcag cgtgtgcagg tcgaggcgct gaagaaacag ctcgagggcc
     2041 gcgagcgcga gttggaggcc gcgcgtctgg acgatccggc ggctgaggcg aagcgctatc
     2101 gcgcgtcggc ggaacttgcg cgcaacgagc agcacgaacg gcaagtgcgg attgcgcagt
     2161 tgcgcagtca actcgagacg gtaggggcgt ccggcctcgg cgagcgtctt gcagcggtca
     2221 aagcggcggt cgagcaagcc acgcgccgca aggacgaact cagcttgcgc gcgagtgcgt
     2281 tgagcttgct ggacgaagtg ctggtcgacg aacgcgatgc agcggtggcg cagttacgcg
     2341 cgccgctgac cgagcggctt gggcactacc tgaagcggat tttcccgcag tcgacgatcg
     2401 cgctcggcga cgatctgagc cccgcgacgc tggatcgtta tggccgcgcg gatacgctcg
     2461 atgcgttgag ctttggtacg cgggaacagc tcggcattct gacgcggcta gcctatgcgg
     2521 atcttttgaa agcatcgggc cgccccacct tgctaatgct cgacgacgcc gccgtgcaca
     2581 ccgatgcggc gcggcgcgat gcgatcaagc gcgcactgat cgatgcggcc acacggcacc
     2641 agatcctggt gttcacgtgc catccggagc tttgggacga tctgggcgtg aggcagcggg
     2701 cgatcgagga tttgaaggtg gcggcttagg ggcctcggct ggtgattaac aacggcttca
     2761 atagtagaag ccgaaatccg acgcgtaccc gtcgtcgtac accggggcgc taaaatccca
     2821 gtcgccggtt ccgtctccgt aaccgtcatc cagtgagaag ttgtactggc cgccgcttga
     2881 cgatattaac gagccgtccc cgttgtaatt tgaccagccg gtttcgaacc cggtcgggcc
     2941 gtaagcgagc gcgtcatagc cgccgccgac cgtgtaggtg aagtcggtga agccggtcaa
     3001 ctgcccctgg ctgttgaacg cgtcgtactg cgtctcgcgg ccggatgcgt cgtagatgat
     3061 ggcgtcctgc gagttgtcgc tgttgaacag gtgttcgacc atgctgccat cgctgccgta
     3121 cgagtcatac tgtgtttccc gaccggtcga accgttgaaa aaatagtcgt cggttttgtg
     3181 gccgctcgtg tcgaacctgg cgaattcggt ttcctggcct gcgctgttgt agacggtcgc
     3241 cgtttgcgta ttgtccgcgt tgaactggtg gatcgtggtt tgattggacg tgtactcgtt
     3301 gtattgcgtg gcgcggccgt tggcgtcgta gaaaatatcg gtggcgagct tgccgttcgt
     3361 tccgtacgtc gcgtactcgg agaggtgtcc cgatggatcg aagacggcgg cgttttgcgt
     3421 gccgtccgga ttgaagatgt gcgcgacgcc gcttccgttc gcgttgtagt cggtctcctg
     3481 gcgttcggcg cccgttttcc catcgaagac gaggtcttta cttatcttgc cgctcgcgtc
     3541 gaaggtggct tgctcggttt catgaccgcc cgcgttgaaa accgttgcgg tctgcgtgcc
     3601 gttggtgttg tagacgcggt cgatctgact gccatcggcg ttgaagtcgt tttcttgcgt
     3661 gagttgtccg gtggtggcgt tgtagaacag gtcctgggtt ttggtgccgt tggcgttgaa
     3721 ggtggcgtat tcggtttcgt gacctgcggc att
//
LOCUS       DATASH010001057         4804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_973862,
            whole genome shotgun sequence.
ACCESSION   DATASH010001057 DATASH010000000
VERSION     DATASH010001057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973862"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            238..1545
                     /locus_tag="VHV99_28035"
     CDS             238..1545
                     /locus_tag="VHV99_28035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX3639263.1"
                     /translation="MQGSFQPAASPRPVAFDSESPAKPKHNAFAAEASASTNLNPSLA
                     DSDNAPAVRLLTDTLQEATRRNASDLHVEPEEHGWRVRLRIDGVLHEIPRPPAHLRDA
                     FITRVKVLARMDIAERRVPQDGRLRLATSPGRVEDYRVNSLPTLFGEKLVLRRLDALP
                     TDLSLDSLGLDPQQRQAVDAAIRAPHGLVLVTGPTGSGKTLSLYCFLHMLNDEARNLC
                     SVEDPAEIQLAGINQVSVREKAGLTFAVALRAFLRQDPDVIMVGEIRDDETADVAVKA
                     AQTGHLVLSTLHTNDAPAAVARLIDIGVEPYNLAAALRMVTAQRLVRRLCVACRAPAP
                     QSTAALRAAGFADDRLDGWQPYGAIGCAACHGIGYRGRVGVHQVMPVSDAMRELIVAR
                     AGSHELARLAQTERVATLRDAALARVRDGTTSLAEALATTEVA"
     gene            1542..2771
                     /locus_tag="VHV99_28040"
     CDS             1542..2771
                     /locus_tag="VHV99_28040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX3639264.1"
                     /translation="MSAAATSPPPVAEMRFRWRGVDADGMRKTGALIAPDVSTARAML
                     KREKLFIVELTARGSAPRPRTRSADVTVFTRQLASLLRAGLPLAPSLDLLAQAQSSRR
                     QGMPRIVGALARDITGGLRFSAALQRHPAQFNALYCQLVEVGEAAGALPAVLARIADD
                     RERAAAQRAKVRAALTYPVAILLLAIAITAALLVWVVPTFKQIFDGFGAKLPAPTQFV
                     LALSAGAARWSIPLVVTIFAAASAVTFLLRRSEAARIRFARLSLTMPVAGPLLRTLCA
                     ARWSRALGTLLSAGTPLADAFHSLTHATGNAFFDRATVEIAARLRRGERLAAAMRAAQ
                     CFPPEVVQPVAVAEESGALDTMLIDVASLADHQVDEKIGTLSSLCEPLVIIVLGTLVG
                     GLVIAMYLPIIQLGNVV"
     gene            2800..3741
                     /locus_tag="VHV99_28045"
     CDS             2800..3741
                     /locus_tag="VHV99_28045"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HEX3639265.1"
                     /translation="MQATIPLVSDTSSSLFSGLLPGHFAGGLGLVFGSLPSGLQIAFA
                     IVFGLVIGSFLNVVVHRVPIMLERAWREEVSETTEVPLEGDGLPARYNLWVPRSACPR
                     CGHVLSAWENLPVLSYLLLRGRCSACKARISLRYPLVEIASAAFAAGALALYGPTGMA
                     FAAFGLCAALLAMSAIDIDTHLLPDSMTLPLLWAGLIVNFNGMFASLHDAVLGAIFGY
                     LVLWAVHWLFRLVRGVEGMGYGDFKLLAALGAWLGWAALPQIVLIAAVAGAVIGLVAT
                     WRGRMRFEEPLPFGPFLAAGGAVTLFLGTPLYLALGG"
     gene            3745..4347
                     /gene="coaE"
                     /locus_tag="VHV99_28050"
     CDS             3745..4347
                     /gene="coaE"
                     /locus_tag="VHV99_28050"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434406.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX3639266.1"
                     /translation="MFSVGLTGGIGSGKSTVADLFAAHGVPLVDTDLIAHRITASHGV
                     AMPQIAAEFGDAFVATDGSLDRARMRTLVFSDDGARKRLESITHPLIRAETEREWYEA
                     QGPYVIVVVPLLVESGSWKTRVNRVLTVDCSVDTQISRVMTRNGFSREQVLAIVARQA
                     TREARLAAADDVIDNDNAPLDALKAQVDAQHRVYLSLAGV"
     gene            4686..>4804
                     /locus_tag="VHV99_28055"
     CDS             4686..>4804
                     /locus_tag="VHV99_28055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HEX3639267.1"
                     /translation="MILYEYPFNERIRTLLRLEDLFERFTFFLTQEDAREHHVA"
BASE COUNT          777 a         1581 c         1527 g          919 t
ORIGIN      
        1 cctctgtgcc ctctgagcac tctgagcact ctgagcactc tgagcactct gagcactctg
       61 agcactctga gcactctgag cactctgagc actctgagca ctctgagcac tctgagcccg
      121 cagcatcgca taccggcgcg atgcgcacca ttagccgatt ggctgaatgg tggtgctggc
      181 ggccttgccg gatgatggcg tcgtcgtgtt ctacaggcgg ctcatcaccg gtctctcatg
      241 caaggatctt ttcaacccgc ggcgtcgccg cggcccgtcg ccttcgatag cgaaagcccg
      301 gccaaaccca agcacaacgc gtttgccgcc gaggccagcg ccagtaccaa cctcaaccca
      361 agtctcgccg attccgacaa cgcacctgcc gtacgcctct tgaccgacac attgcaggag
      421 gcaacccgcc gcaatgcctc ggacctgcac gtcgaaccgg aggagcatgg ttggcgcgtg
      481 cgcttgcgca tcgacggcgt actgcatgaa atccctcggc cgccggcgca tctgcgcgac
      541 gcgttcatca cgcgcgtgaa agtgctggcg cgcatggata tcgccgagcg gcgggtgcca
      601 caggacggcc ggctgcgtct tgccacgtcg cccggccgtg tcgaagacta tcgcgttaac
      661 tcgttgccta ccctgttcgg cgaaaagctg gtgctgcgca ggctcgacgc actgcccacc
      721 gatctttcgc tcgactcgct cggtctcgat ccacagcaac gtcaagcagt cgatgccgcg
      781 attcgcgcgc cgcacggtct ggtactcgtc accgggccca ccggcagcgg caagacgctg
      841 tcgctgtatt gcttcctgca catgctcaac gacgaggcgc gcaatctgtg ctcggtggaa
      901 gatccggcgg aaattcagtt ggcggggatc aatcaggtga gtgtgcgcga aaaggccggc
      961 ctgacattcg cggtggcgct gcgagcgttt ctccgtcagg accccgatgt gatcatggtt
     1021 ggcgaaatcc gcgacgacga aaccgccgat gttgcagtga aggccgcgca gaccggccac
     1081 ctcgtcctgt cgaccttgca caccaatgac gcgcctgctg ccgtcgcgcg cctgatcgac
     1141 atcggcgtgg agccgtacaa cctggccgcc gcgctcagga tggtgaccgc acaacggctc
     1201 gtgcggcggc tttgcgtagc gtgccgcgca cccgcgccgc aatccactgc ggcgctgcgg
     1261 gcagcggggt tcgctgacga tcggctcgac ggctggcaac cctacggtgc catcggttgc
     1321 gcggcttgcc acggcattgg ctatcgcggc cgcgtcgggg ttcaccaggt gatgcccgtc
     1381 tccgacgcga tgcgcgagtt gatcgtggct cgcgcaggca gccatgaact ggcccggctg
     1441 gcgcagaccg aacgggttgc taccttgcgt gacgcggcct tggcgcgtgt gcgcgatggc
     1501 accacgagcc ttgcagaggc gctggccacc accgaggtcg catgagcgcc gctgcgactt
     1561 ctccaccgcc cgtggccgag atgcgcttca gatggcgcgg cgtcgatgcc gacggcatgc
     1621 ggaaaaccgg ggcgctcatc gcgccggacg tcagcactgc gcgagccatg ctcaagcgcg
     1681 aaaagctgtt catcgtcgaa ctcacggcac gcggttcagc gccgcgtccc cgcacccgca
     1741 gcgccgacgt cacggtgttc acccgtcagc tcgccagtct gctgcgcgcc ggtttgccgc
     1801 tcgccccttc gctggatctg ctggcgcaag cgcagagttc gcgccggcaa ggcatgccgc
     1861 gaatcgtcgg cgcgctggcg cgcgacatca ccggtggctt gcgcttctcc gcagcgttgc
     1921 agcggcatcc agcacaattc aacgcgctgt actgtcagct cgtcgaggtc ggcgaagcag
     1981 ccggcgcgct gccggccgtc ctcgcccgca tcgccgacga ccgcgaacgc gccgccgcgc
     2041 agcgtgccaa ggtgcgcgcg gcgctgacct atccggtcgc gatcctgttg ctggcgattg
     2101 cgattaccgc agcgctgctg gtttgggtcg tgccgacctt caagcagatc ttcgacggct
     2161 tcggcgcgaa actgcccgcg cccacgcagt tcgtgctggc attgtcggca ggcgccgcgc
     2221 gatggagcat tcctctagtc gtcacgatct tcgctgcggc ctcggctgtg acgtttctgc
     2281 tacgacgttc cgaagcagcg cgtatccgct tcgcgcgtct gtcgctgaca atgccggtcg
     2341 ccggtccgtt gctgcgaacc ttgtgcgcgg cgcgctggag ccgcgcgctc ggcaccttgc
     2401 tgtcggccgg cacaccgctc gcggatgcgt tccattcact gactcacgcc accggcaacg
     2461 ctttcttcga tcgcgccacc gtggaaattg cggcccgact gcggcgcggc gaacggctcg
     2521 ccgcggcaat gcgcgcggcg cagtgctttc cgccggaggt cgtccagcca gttgcggttg
     2581 ccgaggagtc cggcgcgctc gacacgatgc tgatcgacgt ggcgtccctc gccgatcatc
     2641 aggtagacga aaagatcggc acgctctcga gcttgtgcga gccgctagtc atcatcgtgc
     2701 tgggtacgct ggtcggcggc ctggtgatcg cgatgtatct tcccattatt caactcggca
     2761 acgtggtgta gcatcgcagc cgaatcccac cctttatata tgcaggccac cattccgctc
     2821 gtgtcagata cttcttccag cctgttttcg ggcctgctgc ccggccactt cgcaggcggc
     2881 ctcggcctcg tattcggcag cttgcccagc ggcctgcaga ttgcattcgc gatcgtattc
     2941 ggtctggtga tcggcagctt cctgaacgtg gtggtgcatc gggtgccgat catgctggag
     3001 cgtgcgtggc gcgaggaggt cagcgagacc accgaagtac cgctcgaagg cgacggcctc
     3061 cccgcccgct acaacctgtg ggtgccgcgc agcgcgtgcc ctcgctgcgg ccacgtgctc
     3121 agcgcgtggg agaacctgcc ggtattgagc tatctgctgt tgcgcgggcg ctgctccgca
     3181 tgcaaggcac gcataagctt gcgctacccg ctggttgaaa tcgccagcgc cgccttcgcg
     3241 gccggggcgc tcgcgctgta tgggccgacc ggcatggcat ttgccgcctt cggactgtgc
     3301 gccgcactgc ttgcgatgag cgcgatcgac atcgacaccc atctgctgcc tgactccatg
     3361 acgctgccgc ttctgtgggc cggcttgatt gtgaacttca acggcatgtt cgcgagcctc
     3421 cacgatgccg tgctgggcgc gatcttcggc tatctggtgc tgtgggccgt gcactggctg
     3481 ttccggctgg tgcgcggcgt cgaggggatg ggttatggcg atttcaaact actggccgcg
     3541 ctgggcgcat ggctcggctg ggcggccctg ccgcagatcg tgctgattgc ggcggtcgca
     3601 ggcgcggtaa tcggtctcgt ggcgacgtgg cgcggccgca tgcgcttcga agagccgctg
     3661 ccgttcgggc cgtttctcgc tgcgggcggt gctgttacgc tatttctcgg cacaccgctt
     3721 tatctggcgt tggggggctg aaccatgttt tctgtgggat tgacaggtgg catcggcagc
     3781 ggcaaatcaa ccgtcgccga tctgttcgcc gcacatggcg tgccccttgt cgacacggac
     3841 ttgatcgcgc atcgcatcac ggcgtcgcac ggtgtcgcga tgccgcagat cgccgcggag
     3901 ttcggcgatg cgttcgttgc cacggacggc tcgctcgacc gcgcccgcat gcgcacgctg
     3961 gtgttcagcg acgacggcgc gcgcaaacgg ctcgaaagca tcacgcatcc gttgattcgt
     4021 gcggagactg aacgcgagtg gtatgaggcg caagggccgt atgtgatcgt ggtggtgccc
     4081 ttgctggtgg aatcgggtag ctggaagacc cgcgtgaatc gtgtgttgac cgtcgattgc
     4141 agcgtcgaca cgcaaatttc gcgtgtgatg actcgcaacg gcttcagccg tgaacaggtg
     4201 ctggcaatcg tcgcccgcca ggcgacgcgc gaagcgcgcc tcgccgccgc cgacgacgtc
     4261 atcgacaacg acaacgcgcc gctcgacgcg cttaaggcgc aggttgatgc gcagcatcgc
     4321 gtctatctgt cgctagcggg tgtttgagaa tgcacggacc gggacttgcc aggcggtatc
     4381 gccgataggc gttcagatag gcgttcaatg agcccgtagg aaacgacaca tggatgccac
     4441 atacgtacaa cgtgagcgtg caataagccc tgcgtaggcg gcacatgcac ccatacacac
     4501 catgtctctt gcccatcatc cattgaactt tgccgcggca cccgagccga agcacgcaga
     4561 aaccgcccga aacgcgtgaa aaaagttgcg aaagcgcgct caaaaagtgc gtgattgcta
     4621 gggtagtctc acggcattag aatgttctgc attcccgtca ccgaccacgc ccgaggcgag
     4681 cccgcttgat cctttacgag tatcccttca acgagcgaat ccggacgcta ttgcgcctcg
     4741 aagatctgtt cgagcgcttc acgttctttc tgactcagga agacgccagg gaacatcacg
     4801 tcgc
//
LOCUS       DATASH010001058         6602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2360488,
            whole genome shotgun sequence.
ACCESSION   DATASH010001058 DATASH010000000
VERSION     DATASH010001058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6602
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2360488"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(214..1080)
                     /gene="radC"
                     /locus_tag="VHV99_28060"
     CDS             complement(214..1080)
                     /gene="radC"
                     /locus_tag="VHV99_28060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011851209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEX3639268.1"
                     /translation="MACYARTTDHATLEATPPKPGASAAPAAAVVLPPTCAAVGKSAP
                     GSNASIADAIANAGAPPARQLVGGKWLKSDMPRERLIEEGPGVLSDTEMIVLVLGSGL
                     PGHDVFNVARTLLDRFGSLRAMLDATYSDFEGLRGIGPAKKAQLIAIMEMARRSLVDK
                     LRDRSLMNSPEAVQDYLRLRIGGMTQEVFVSLYLDARHRLIRCEESARGTLTRMAVYP
                     REIVRRALTLNAASLIVAHNHPSGAVQPSASDCRLTHTLREALALIDVQLIDHLVIGA
                     DNTYSFARAGWP"
     gene            1291..1746
                     /locus_tag="VHV99_28065"
     CDS             1291..1746
                     /locus_tag="VHV99_28065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX3639269.1"
                     /translation="MSIIDISEVKPGSHVTLHYRLSLADGAEVINTFTEKPATLLLGA
                     GQLAPPLEDILLGLKVGHHSTFQLTPGQAFGPRNPELIQRVSLATLRENSMIGEDFSP
                     GDLVEFNAPGGGRYAGVLKEVGETSALLDFNHPLAGQALAFEVKIIGIL"
     gene            1749..2708
                     /gene="ispH"
                     /locus_tag="VHV99_28070"
                     /gene_synonym="lytB"
     CDS             1749..2708
                     /gene="ispH"
                     /locus_tag="VHV99_28070"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX3639270.1"
                     /translation="MSTTDTTLAEAEILLAQPRGFCAGVDRAIEIVERAIKLHGSPIY
                     VRHEIVHNAYVVEDLRKKGAIFIEQLEEVPAGNTVIFSAHGVSKAVRAEAESRGLRVY
                     DATCPLVTKVHIEVAKMRQDGFDIVMIGHKGHPEVEGTMGQAGEGMHLVEDIADVQAL
                     HLAEPERIAFVTQTTLSVDDAAEIIGALKAKYPAIREPKKQDICYATQNRQDAVKFMA
                     PQCDVVIVVGSPNSSNSNRLRELAEKLGVPSYMVDSPDQIDPVWVEGKRRIGVTAGAS
                     APEALAQAVIGRLRELGVRNVRALEGIEENIAFPLPRGLGLPA"
     gene            3253..4452
                     /locus_tag="VHV99_28075"
     CDS             3253..4452
                     /locus_tag="VHV99_28075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX3639271.1"
                     /translation="MRIKFAYAVSIVTAVAMLTACGKKPDGEAGASASATTAAAAPAS
                     EATVVKIGHAAPLTGPIAHLGKDNENGARLAVEEINRQGLTIDGRKIQLELDAQDDAA
                     DPKTGTAVAEKFVDDHVVAVVGHLNSGVSIPASKIYSDAGIVQISPSSTNPGYTQQGF
                     KTTYRVVATDAQQGPALANYATKVLGAKRIAIVDDSSVYGKGLADEFAKTVQASGAKI
                     VAREATNDRATEFQAVLRKIRSVQPDVIMFGGMDATGGPFTKQAAALGIRAKILGGDG
                     VCTDKVSELAGTAVQNLVCSEAGLALSKMDRGADFEKKYVDRFHTPVQIYAPFTYDAV
                     YVIVDAMKRANSIEAPKVLAAMPSTDYNGVIGHIAFDDKGDLKEGAITLYDFKDGKKA
                     VLDVVKM"
     gene            4682..5632
                     /locus_tag="VHV99_28080"
     CDS             4682..5632
                     /locus_tag="VHV99_28080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011885761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX3639272.1"
                     /translation="MDIFVQQVLNGLVLGSVYAIIALGYTMVYGILGIINFAHGDVLM
                     VGAMVALSAIGVLQNHFPGLGNVPTLIIALIIAAVVCSLVGYTIERVAYRPLRKAPRL
                     APLITAIGVSILLQTLAMMIWSRNPLPFPQLLPTDPINVIKATDTTPGAVISMTEIVI
                     IVVAFLVMGGLLLLVHKTRLGRAMRAIAENPGVASLMGVNPNFVISATFMIGSALAAL
                     AGVMIASEYGNAHFYMGFIPGLKAFTAAVLGGIGNLGGAMVGGVLLGLIEQLGAGYIG
                     NLTGGVFGSNYQDVFAFIVLIIVLVFRPSGLLGERVADRA"
     gene            5651..>6602
                     /locus_tag="VHV99_28085"
     CDS             5651..>6602
                     /locus_tag="VHV99_28085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006478052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3639273.1"
                     /translation="MTSIQPIEPSTTLIPEKNLTKTLTVGIITAIFVIAAPMIIGAAG
                     GNYWVRVLDFAMLYVMLALGLNVVVGFAGLLDLGYIAFYAIGAYVAALLSSPHLTSQF
                     EWIAHLFPGGLHAPIWIIVPIAMALAATFGVLLGAPTLRLRGDYLAIVTLGFGEIVRI
                     FMNNLDRPVNITNGPKGITGIDPVSVGGFSLAQTHSLFGLQFPSVYSYYYLFVLCALF
                     VIWTCTRLQHSRIGRAWAAIREDEIAAKAMGINTRNVKLLAFAMGASFGGLSGAMFGS
                     FQGFVSPESFTFWESVVVLACVVLGGMGHIPGVILGAVLLA"
BASE COUNT         1274 a         1921 c         2086 g         1321 t
ORIGIN      
        1 tgagttcgaa cgccgtgaaa gtttcccgaa aagagccgcg cttaagtttt tgaacttcag
       61 tcgcttttcc tttccggaac gccttggtgg cttcggaaca gggggttgga aataggcaaa
      121 ccggaattat agcggaaaaa aagccgcaaa atcaaacctt atttgcgata ccgaacacgg
      181 cgcagcgttg tcgtaaccct ggttggcgcc gtgtcacggc cagccggcgc gcgcaaacga
      241 ataggtgttg tcggcgccga tcaccagatg atcgatcagt tgcacgtcga tcagcgcaag
      301 ggcctcgcgt agcgtatgcg tcagccggca gtcgcttgcg ctcggctgga cggcgccgga
      361 agggtgatta tgcgcgacga tcagactggc ggcattcaga gtcagcgctc gccgcacgat
      421 ctcacgcgga tacaccgcca tacgtgtcag cgtgccgcgc gcgctttcct cgcatcggat
      481 caaccgatgc cgggcgtcga gatagagcga cacgaagacc tcttgcgtca tgccgccgat
      541 gcgcagccgc aaataatcct gcacggcctc gggcgagttc atcagcgaac ggtcacgcag
      601 tttatcgacc agcgaacgtc gtgccatctc catgatggcg atcaactgtg ccttttttgc
      661 cggaccgatg ccgcgcaggc cttcgaagtc cgagtaagtt gcgtcgagca ttgcccgtag
      721 cgagccgaag cggtcgagca gggtgcgtgc gacgttaaac acgtcgtggc cagggaggcc
      781 cgagccgagc accagcacga tcatttcggt gtcggacagc acgcccgggc cttcctctat
      841 cagccgctcg cgcggcatgt cgctttttag ccatttgccg cctacgagct gtcgggccgg
      901 gggtgcgccg gcgttcgcga tggcgtcggc gatgctagcg ttactgccgg gtgcggattt
      961 gccaactgcg gcgcaagtgg gcggcaatac cacggcggct gccggggcgg cggacgcgcc
     1021 aggcttcggc ggggtcgctt cgagtgtcgc gtggtctgtc gtgcgggcat agcaggccat
     1081 atagataaga atcctccagt ttgggttggc ggcggcgcac gggggccgcc gcccaggcct
     1141 cgtgccgcgg tacgcgcgac ggagccgagc cgccgcccgc tatgtggctt acaatagaca
     1201 ctttgcgcat ggcaccccga cggtctgcca cacgcgtcga ctgcgcccgc ggcgcacgcg
     1261 tgcagcgcgc ttcgacagag cgagcattgc atgagcatca tcgacatttc cgaagtgaaa
     1321 cccggttcac acgtgacgct tcactaccgg ctttcccttg ccgatggcgc tgaagtcatc
     1381 aatacattta ccgaaaagcc ggccacgctg ctgctcggcg cgggtcagct ggcgccgcca
     1441 ctggaagata ttttgctggg tttgaaggta ggccaccatt cgacctttca gctaacgccg
     1501 ggccaggcat tcggtccgcg caatccagag ctgatccagc gagtttcact ggccaccctg
     1561 cgcgagaaca gcatgatcgg cgaggatttt tcgcccggcg atctggtcga attcaacgcg
     1621 ccgggcggcg gccgttacgc gggcgtcctg aaggaggtgg gcgaaacgtc ggcgctgtta
     1681 gatttcaacc atccgctcgc cggccaggcg ctggcgtttg aagtgaaaat catcgggatt
     1741 ctgtaaacat gagcaccacg gatacgactc ttgccgaagc cgagatcctg ctcgcacagc
     1801 cgcgtgggtt ctgcgccggc gtcgatcggg ccatcgaaat cgtggagcgg gccatcaagc
     1861 tgcacggctc gccgatctac gtgcgccacg aaattgtcca taacgcctat gtcgtcgagg
     1921 atctgcgcaa aaagggcgcc atcttcatcg aacagctgga agaagtcccg gccggcaata
     1981 cggtcatttt cagcgcgcat ggcgtgtcga aagcggtgcg ggcggaagcc gagtcgcgcg
     2041 gcctgcgggt gtacgacgcc acctgtccgc tcgtcaccaa ggtgcatatt gaagtcgcga
     2101 agatgcgcca ggacggcttc gacatcgtga tgatcggcca taagggccac cctgaagtcg
     2161 agggcacgat ggggcaagcc ggcgaaggca tgcacctggt cgaagacatc gcagacgtac
     2221 aggccttgca cttggccgag cccgagcgga tcgcgttcgt cacgcaaacc accctgtcgg
     2281 tcgacgacgc agccgagatc atcggtgcct tgaaggcgaa atatccggcg atccgtgagc
     2341 cgaagaagca ggacatctgt tacgccacgc aaaatcgtca ggatgcggtc aagttcatgg
     2401 cgccgcagtg cgacgtcgtg atcgtggtcg gcagcccgaa tagttcgaac tcgaaccggc
     2461 tgcgcgagct ggctgaaaag ctcggcgtgc cttcctatat ggtggactcg ccggaccaga
     2521 tcgatccggt ctgggtcgaa ggcaagcgcc ggatcggcgt aacggcgggc gcgtcggcac
     2581 cggaagcgct ggcgcaggcg gtaattggcc ggttgcgcga gcttggtgtg cgcaacgtgc
     2641 gcgcgctcga aggcattgag gaaaacatcg cgtttccgtt gccgcggggg ctgggcctgc
     2701 ctgcctgaca atcagtcaac gtttctgaaa gaaaggcgcg ccctagggcg cgcttttttt
     2761 acgcccgcgg attcatggcg ctctcctgaa taaagtgcca gatcttcggc aggtgctgtt
     2821 tttagtgtcc ttccacacag attcttttaa attgccattt gtcttttaat cgatatttca
     2881 ttcatgtggc cctgtttaat tcggagccaa ggcgttgcgt cgatttttga cgcactaaaa
     2941 tagtgcgtac gctttgatac caatggaaac gtaatactta acgcgaacca cgctgccaat
     3001 cagggatcag gccatatccc tgcaacagtt gatgccattg ggtgcagcgg tggtgcaact
     3061 gatgcgcgga aaatagtcgc aaccgggttg cacctattgc cggacttatc tttaaaaata
     3121 aggttgcaat gcaggaaaac cccaccggtt caacaatatt gcacgtcgca taattggagt
     3181 tacaatgcgc gcgttctcaa ggcctccggg tcccgaacaa cggattttgc aaggcgcggg
     3241 agacctactt aaatgcgaat caagtttgct tacgccgtgt ccatcgtgac cgcggttgcg
     3301 atgttgaccg catgcggcaa gaaaccggat ggcgaagcgg gagcaagtgc gtcggctacc
     3361 acggcggcag cggcaccggc gagcgaggcg actgtcgtga aaatcggtca tgcggcccca
     3421 ttgacggggc ccattgcgca tctcggtaaa gacaatgaaa acggcgcgcg tctggctgtc
     3481 gaggaaatca acaggcaggg tttgactatc gacggtcgca agatccagct ggagctcgac
     3541 gcgcaggacg atgccgcgga cccgaaaacg ggaacggcgg tcgctgaaaa attcgtcgac
     3601 gatcatgtcg tcgccgtggt cggccacttg aattcggggg tctcgattcc ggcgtcgaag
     3661 atctatagcg atgccgggat cgtacagatc tcgccgtctt cgacgaaccc ggggtacacg
     3721 cagcaaggtt tcaagacgac ttatcgggtg gtcgcgaccg atgcccagca ggggccggcg
     3781 cttgccaact acgcaacgaa agtgcttggc gcaaaacgca tcgcgatcgt cgacgactcg
     3841 agcgtatacg gtaagggact ggccgacgag ttcgcgaaga cggtacaggc gagcggcgca
     3901 aagatcgtcg cgcgcgaagc cacgaatgac agggctacgg agtttcaggc cgtccttaga
     3961 aagatcagga gtgttcagcc ggacgtgatc atgttcggcg gcatggacgc aacgggcggg
     4021 ccgttcacca aacaggcggc cgcgctcggt atcagggcaa aaatccttgg cggcgacggc
     4081 gtgtgtaccg acaaggtgag tgagctggcg ggaaccgccg tgcaaaacct ggtctgttcg
     4141 gaggcgggac tcgcgctttc gaaaatggac aggggagcgg acttcgaaaa gaagtacgtc
     4201 gaccgcttcc acacgccggt gcagatttac gcaccgttca cgtatgacgc tgtgtacgtg
     4261 attgtcgatg caatgaagcg cgctaattcg atcgaggcgc ccaaggtgct ggctgcgatg
     4321 ccgtccaccg attacaacgg ggtaatcggc cacatcgcgt tcgacgacaa gggtgatttg
     4381 aaagagggcg ccattacgct ttacgacttc aaggacggca agaaagcggt tctcgacgtc
     4441 gtgaagatgt gatgacggga cgttcgactg acggcaccga aaccacccaa cggtgccgtt
     4501 tttgcatccg ccatcggcga gtccccgact gcatcccagt cgtaagacga accaggcagc
     4561 ggataaccct taccggtctt ttacatcagt acccgcagtg ccgttgagat tggcgtaccg
     4621 catcaccgcc caccggctga tgaagaacgc gaatccagtc gcacgggcta aggagcatta
     4681 aatggatatc ttcgtccagc aggtcctcaa cgggctagtg cttggcagcg tttacgccat
     4741 catcgcactg ggctatacga tggtttacgg catcctgggc atcatcaact tcgcccacgg
     4801 cgatgtattg atggtgggcg cgatggttgc gctttcggcc ataggcgtgc tgcagaacca
     4861 cttccccggc ctcggcaatg tgccgacgct catcatcgcg ttgatcatcg cggcagtggt
     4921 ttgctcgctg gtcggctaca cgatcgagcg cgtggcctac cggccgttgc gtaaagcgcc
     4981 gcgtctcgcc ccgctgatca cggcgatcgg cgtgtcgatc ctgctgcaaa cgctcgcgat
     5041 gatgatctgg tcgcgcaatc cgctgccgtt cccgcagctg ttgcccaccg acccgatcaa
     5101 tgtgatcaag gccaccgaca cgacgcctgg cgccgtgata tcgatgactg aaatcgtgat
     5161 catcgtggtg gcgttcctcg tgatgggcgg tctgctgctg ctggttcaca agaccaggct
     5221 gggccgcgcg atgcgtgcga tcgccgagaa tccgggcgtt gccagcctga tgggtgtgaa
     5281 cccgaacttc gtgatttcgg cgaccttcat gatcggctcg gcgcttgccg ctctggccgg
     5341 cgtgatgatc gcgtccgaat acggcaacgc tcacttctat atgggcttca tcccgggttt
     5401 gaaggccttt accgcggcgg tgctgggcgg catcggcaat ctcggcgggg cgatggtggg
     5461 cggcgtgctg ctcggcctga tcgaacagtt gggggcgggc tacatcggca acctgaccgg
     5521 cggtgtgttc ggcagtaact atcaggacgt gttcgcgttc atcgtgctga tcatcgtgct
     5581 ggtgttccgt ccgtcgggcc tgctcggcga acgtgttgcg gatcgcgcct gatccaggga
     5641 gaaaacagac atgacctcaa ttcaaccgat cgagccgtcc acgacgctca tccctgaaaa
     5701 gaacctgacg aagacgctga ccgtcggcat catcaccgcg attttcgtga tcgcggcgcc
     5761 gatgatcatc ggcgcggccg gcggcaacta ctgggtccgc gtgctcgact tcgcgatgct
     5821 gtacgtaatg ctcgcgctgg gcctcaatgt ggtggtcggc ttcgccggcc tgctggacct
     5881 gggctacatc gcgttctacg ccatcggcgc gtacgtcgcg gcgctgctga gctcgcccca
     5941 cctgacctcg cagttcgagt ggatcgcgca cctgttcccg ggcggcctgc atgcgccgat
     6001 ctggatcatc gtgccgatcg cgatggcgct cgccgcgacc ttcggcgtgc tgctcggcgc
     6061 accaacgttg cgtctgcgtg gtgactacct cgccatcgtg accctgggct tcggggaaat
     6121 cgtgcggatc ttcatgaaca acctcgaccg tccggtgaac atcaccaacg gaccgaaggg
     6181 gatcacgggg atcgatccgg tgtcggtggg cggtttcagt cttgcgcaga cgcactcgct
     6241 gttcggcctt cagttcccct cggtgtactc gtactactac ctattcgtgc tgtgcgcgtt
     6301 gttcgtgatc tggacctgta cgcgtttgca gcactcgcgt atcggccgtg cctgggccgc
     6361 gatccgtgaa gacgaaatcg ccgccaaggc gatgggcatc aacacccgca acgtgaagct
     6421 gctggcgttc gcgatgggcg cgtcgttcgg cggcctgtcg ggcgcgatgt tcggttcatt
     6481 ccagggcttc gtgtcgccgg aatcgttcac cttctgggag tcggtggtgg tgctggcgtg
     6541 cgtggtgctt ggcggcatgg gccatattcc tggcgtcatt ctcggcgcgg tgctgctcgc
     6601 gg
//
LOCUS       DATASH010001059         2649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4734180,
            whole genome shotgun sequence.
ACCESSION   DATASH010001059 DATASH010000000
VERSION     DATASH010001059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4734180"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="VHV99_28090"
     CDS             <1..514
                     /locus_tag="VHV99_28090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEX3639274.1"
                     /translation="DGRLHGGPHDNAGAVGSIPLKDGSARKPARQLLHAASGFVLEQL
                     LSNAGAPSAAAHDHRALSPELWRQTEQWLDTACPAIASALTNAAALLDLEAVVIDGEL
                     DRQLVREIIRRTERVLDRFEWEGMVRPQLLEGTIGADARAMGGAILPLYAHFAPVHEL
                     FLKPATDAGY"
     gene            complement(524..>2649)
                     /locus_tag="VHV99_28095"
     CDS             complement(524..>2649)
                     /locus_tag="VHV99_28095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase family 51"
                     /protein_id="HEX3639275.1"
                     /translation="GKAFREVLSLMIAQRAPSHFLNHGYAELERLTDSYLRLLATGGV
                     ISIPLRDAALAAQVELHRAPAGTPSTDSFVSRKAVTSMRSHLLAALGIPSFYELDRLD
                     LQAKGTLNNAVQQAVSERLAAAATRDGAKAAGLVGFEMLRPSDDPSRIAYSFTLFERR
                     DGANLVRVQTDSVNQPFDINSGARLNLGSTAKLRTVVTYLQIVSDLHARYAPLSTAEL
                     KAVKPDPNDQLTRWALDYLTHTSDRSLQAMLDAAVERKYSASAGETFYTGGGAQTFNN
                     FEADDNGRILTVHRAFQHSVNLVFVRLMRDIVHYEMVQTTGPSSQWLGDPAIRKMYLT
                     RFADQESRVYMNRFYTRYHGKTPDQALSLLLLGVRKSPPKVATVLRSVAPDESNAWFN
                     AKMRAALKNTPAASVLDDEDLANLYAKYAIDRFNLNDRGYISSMHPLELWTLNYLRAH
                     PEATLDQVQKASSEVRLSTYSWLFKTRYHATQDRRIKRMVELRAYDAIDKSWQALGYP
                     FATLTPSYAAAIGASGDRPAALAQLIGVIANGGNKVPTESLTQLDFAKDTPYETHFQR
                     AAVAPQQQLSPEIAGVVRVLLRDVVLGGTARRLAQGMTFPNGQTLEIYGKTGTGDQRF
                     NVWAKGARLIESRKVNRSATFVFVMGDRFYGTLTAYVHEPYAARYEFTSALSVQLLKS
                     LAPVLQPLLQNPPAKTASITKVTAQ"
BASE COUNT          485 a          804 c          847 g          513 t
ORIGIN      
        1 cgacggccgt ttgcatggcg gaccacacga caacgcaggc gcggtaggct cgattccgct
       61 gaaagacggt agcgcgcgca aaccggcgcg gcaactgctg cacgcggcat cgggttttgt
      121 tctggaacag ttgttgagta atgcgggggc gccgtccgct gccgcgcacg atcatcgcgc
      181 attgtcgccg gaactgtggc ggcagactga acaatggctc gacaccgcct gccccgcgat
      241 cgccagtgcg ttgaccaatg cagccgccct gctcgacctg gaagcggtgg tgatcgacgg
      301 cgaactcgac cggcaactgg tccgcgaaat catccggcgg accgagcgcg tgctcgatcg
      361 ctttgaatgg gagggcatgg tgcgcccgca actgttggaa ggcaccatcg gtgccgacgc
      421 acgcgcgatg ggtggtgcga ttctgccgtt gtacgcgcat ttcgcgcccg tgcacgagct
      481 attcctcaag ccggcgacgg acgcgggtta ttagccgtcg ttgctattgc gcggtcacct
      541 ttgtgataga agcggtcttg gcaggcgggt tttgaagcaa cggctgcaac accggcgcga
      601 gcgacttcag caactgtacc gagagcgcgc tggtgaactc gtaacgcgcc gcatacggtt
      661 catgcacgta ggcagtcagc gtgccataga agcggtctcc catcacaaac acgaacgtcg
      721 cgctgcgatt caccttgcgc gactcgatca gccgcgcgcc tttggcccac acattgaagc
      781 gctgatcgcc ggtgccggtc ttgccgtaaa tctcgagtgt ctgaccattg gggaacgtca
      841 tgccctgcgc gaggcgcctg gccgtgccac ccaatacgac atcgcgcaac agcactctga
      901 ccaccccggc gatttccggc gacaactgct gttgcggcgc caccgcagcc cgttgaaaat
      961 gtgtttcgta tggcgtgtct ttcgcgaaat cgagttgcgt aaggctttcg gtcggcacct
     1021 tgttgccgcc gttggcaatc acgccgatca gctgcgcgag cgcggccggc cgatcaccgg
     1081 atgcaccaat cgccgctgca tacgagggcg tgagcgtcgc gaacggatag ccaagcgcct
     1141 gccacgactt gtcgattgca tcgtaggcgc gcagctcgac catccgcttg atgcgacgat
     1201 cctgagtggc gtgatagcgc gtcttgaaga gccacgaata cgtcgacaga cgcacctcac
     1261 tgctcgcttt ctgtacctga tcgagcgtcg cctcgggatg ggcgcgcaga tagttgagcg
     1321 tccacaattc cagcgggtgc atgctcgaaa tgtagccgcg gtcgttcaga ttgaaacggt
     1381 cgatggcgta tttggcgtac agattggcca gatcctcgtc gtccagcacg gaggcagccg
     1441 gtgtgttctt cagcgcggca cgcatcttcg cgttgaacca tgcattcgat tcatccggcg
     1501 cgacgctgcg cagcacggtc gcgactttag gcggcgattt gcgtacgccg agcaacagca
     1561 aggagagcgc ctgatcgggt gttttgccgt ggtacctggt gtagaagcga ttcatgtaca
     1621 cacggctttc ctgatcggcg aatcgcgtga gatacatctt gcggatcgcc ggatcgccca
     1681 gccattgcga cgacggcccg gtggtctgca ccatctcata gtggacgata tcgcgcatca
     1741 gccgcacgaa caccagattc accgaatgct ggaatgcgcg gtgcacggtg agaatgcggc
     1801 cgttgtcgtc ggcttcgaag ttgttgaagg tttgcgcgcc gccgcccgtg tagaaggttt
     1861 caccggcgct tgccgagtat ttgcgctcga cggccgcatc gagcatggct tgcagcgagc
     1921 ggtccgaggt atgcgtcaga taatcgagcg cccagcgcgt gagctgatcg ttcggatcgg
     1981 gcttcacggc tttcagctcc gctgtgctca gcggtgcata acgcgcgtgc aggtcggaca
     2041 cgatctgcaa atacgtgacc acggtgcgca gtttcgcggt ggaaccaaga ttcaggcgcg
     2101 cgccggaatt gatgtcgaac ggctggttga cgctgtccgt ttgcacacgc acgaggttcg
     2161 cgccgtcgcg ccgttcgaac agcgtgaagc tgtacgcgat tctcgaggga tcgtccgaag
     2221 ggcgcagcat ttcgaaaccc acgaggcccg cggctttcgc gccatcacgc gtcgcggcgg
     2281 ccgcgagccg ttcgctgacg gcctgctgga ccgcgttgtt gagcgtgccc ttcgcctgca
     2341 gatcgagtcg atcgagttca tagaaactcg gaatgccgag tgccgccagc agatgcgagc
     2401 gcatcgacgt gacggccttg cgcgacacga acgaatcggt actcggcgtt ccggcgggcg
     2461 cgcggtgcag ttcgacctgc gcggccagcg ccgcgtcgcg cagcggaatc gaaattacgc
     2521 cgccggtcgc cagcaatcgc agataactgt cggtcagccg ttcgagttcg gcatagccgt
     2581 gattcaggaa atgcgacggc gcgcgttgcg cgatcatcag cgacagcact tcgcggaacg
     2641 ccttgccct
//
LOCUS       DATASH010001060         8438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1767598,
            whole genome shotgun sequence.
ACCESSION   DATASH010001060 DATASH010000000
VERSION     DATASH010001060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 8438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8438
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1767598"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..168)
                     /locus_tag="VHV99_28100"
     CDS             complement(<1..168)
                     /locus_tag="VHV99_28100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014973236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HEX3639276.1"
                     /translation="MQVCAAPPFAADHALSRDARWRPHDLLRLQRLRQFEGEPAWVRA
                     TFERAPYAVVRR"
     gene            complement(168..983)
                     /gene="mdcE"
                     /locus_tag="VHV99_28105"
     CDS             complement(168..983)
                     /gene="mdcE"
                     /locus_tag="VHV99_28105"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit gamma"
                     /protein_id="HEX3639277.1"
                     /translation="MNNTTLSRGACWFHALAGEPSGKAPVWSGDAPLGGETARFIAVV
                     PDLDNRFPRATDNVVGLEQGWQLARAVRETIAQDASSGTRRPIIAIVDVKSQAYGYRE
                     EMLGIHLACAAAVDAYASARDAGHPVIALIVGPAMSGAFLAHGYQANRIVALDAPGTM
                     VHAMGKEAAARVTRRTVEALDALGETIVPMSYSMTSFAKLGLLDQLIEGVDADAPDAA
                     QIEHVREVLTGQIQCARDGKPDLSHRLESAAAQKNRAASIEVRRRLAEQWDAV"
     gene            complement(980..1867)
                     /locus_tag="VHV99_28110"
     CDS             complement(980..1867)
                     /locus_tag="VHV99_28110"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HEX3639278.1"
                     /translation="MNTVDTARPAPMLRESFIELPARERARSLLDAGTFRELLGPFDK
                     IESPWLPLQGVVCQADDGCVIARGTIDGEPAVVAAIESAFQGGSIGEVSGSKIAAALE
                     MALHDCERGKLVRPVVLFETGGVRLQEANLGLAVIAEMQAAIVALRRHVPVVGVIAGM
                     VGCFGGMSLAAALCSYLVVTKQGRLGMNGPEVIEQEAGIEELDASDRRRVWQLIGGEQ
                     RAATALADQLVDDDANAVRAAVRSAFTQGVPAAHRSEQVDTFLGRLARIDPASVTPEA
                     MRDVYNPGYNPQMSGKEQA"
     gene            complement(1864..2178)
                     /locus_tag="VHV99_28115"
     CDS             complement(1864..2178)
                     /locus_tag="VHV99_28115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HEX3639279.1"
                     /translation="MEHLTFDYPAQRAVTTRAHVGVVGSGDLEVLLSPSDNAGALAAH
                     VLVRTSVDGYSHIWKSVLDRFFTRYDGAAQIEINDFGATPGVVALRLAEAIEAAEQGD
                     DA"
     gene            complement(2166..3101)
                     /locus_tag="VHV99_28120"
     CDS             complement(2166..3101)
                     /locus_tag="VHV99_28120"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HEX3639280.1"
                     /translation="MGSREALLVRAVGAAVAAQPGLSDVQLACYAVNALIEEAQLTPK
                     PALVDRRGSGAHRDLDLATMLRSAHALEPTFAALAHSARRRGEPSALLRAELAQIGRA
                     GEQAMLHATGGSNAHRGAIWIVCLLVAGASMRSVSMPGAAAPLNASHICTHAAQIACF
                     PDRFAAPADSHGERARQRYQVGGARREAQDGFPHVIDIGLPALLAARAGGVDENAARI
                     DALLSIMASLDDTCLLHRAGLPGLHAAQRGARRVLQAGGSSTRDGHAALAALEHELLS
                     LNASPGGAADLLAATLFLDMLAHHDAGGSSASWNI"
     gene            complement(3104..4765)
                     /gene="mdcA"
                     /locus_tag="VHV99_28125"
     CDS             complement(3104..4765)
                     /gene="mdcA"
                     /locus_tag="VHV99_28125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HEX3639281.1"
                     /translation="MNRPAEASPTARSWTTRRDEKNRRLAAIAPWLEDGILPTNRIVD
                     ALETLIQPGDRVALEGDNQKQADFLSRSLARVDPQKIHDVHLLISSIGRPEHLTLFER
                     GIAHKVDFSFAGPQSLRVAQLLEDGQLEIGAIYTYVELYARMFVDLTPHVALLCAEKA
                     DRHGNLYTGANTEDTPTIAEAAAFRHGIVIVQVNEIVDELPRVDIPGSWVDVVVQADT
                     PFAVEPLFTRDPRHIGDLQVLTAMMVIRGIYEPYGVSSLNHGIGFDTAAIELLLPTYG
                     ESLGLKGKICRNWTLNPHPTMIPAIESGWVDSIHCFGSEVGMEAYIEARPDVFFTGND
                     GSLRSNRVLCQLAGQYGVDLFIGSTLQIDADANSSTVTRGRLAGFGGAPNMGHDPRGR
                     RHSSEAWLKLLKDQGPVSRGHKLVVQMAETYKKGGESTFVDELDAVAVGAKSGMPIAP
                     VMIYGDDVSHVVTEEGIAYLHKAEGIDERRAALAAVAGVTPIGLRARPEKTAELRRRG
                     IVAYPEDLGIRRGEAKRSLLAARSIDDLVTWSGGLYEPPARFRSW"
     gene            complement(4774..5547)
                     /locus_tag="VHV99_28130"
     CDS             complement(4774..5547)
                     /locus_tag="VHV99_28130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX3639282.1"
                     /translation="MSDAIDGFPLDAPARSPLLPAAPPRESTSRVIAEALRAAIVDGT
                     LAPGAPLRQDAIARHFSVSAIPVREALRQLESEGWARAAVHKGATVAPLSADEAREIY
                     EIRSALESLAIGLAIPVHTAATLRECARLCRAAEREADPSLYVARNVAFHMSLYAPAA
                     RPQLEDMIGTLHRRGERYLRLKFGLPSYKGESDNEHAALLDAVQRRDIPAAQSLVVTH
                     LLGTGDLLHRFLTERAQAEAALANQPKPRARRTRTTTGS"
     gene            5740..7038
                     /locus_tag="VHV99_28135"
     CDS             5740..7038
                     /locus_tag="VHV99_28135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3639283.1"
                     /translation="MNSLTDRTPVARRRTPLNRSQIAGFWGAWAGWTLDGMDSFIYAL
                     VLTPALTELLPRSGYAATPANVGLAGSILFALFLVGWGLSFIWGPLADRFGRTKVLAG
                     TIFTFAIFTGLAATSHNVWELAIYRFIAGVGIGGEWALAGTYVAEAWPEDRRKMGAGY
                     LQTGYYAGFFLAAALNYTIGVHYGWRAMFLTGAVPVVVAILVLLRVKESEKWEKAEAG
                     TVRAKPLREILGPMYRRRTWVACILLTIAIIGLWAGAVYEPSAVIQLATKAGMAKNDA
                     IRTASLATGLLSIATILGCLALPPLAERFGRKKTLAIYFIGMAVSIVGSFGWAFYLPN
                     GLAPFIAWLFVLGFFGGNFALFSLWLPEQFETRVRATAFAFCTSFGRFVGAGVNFLLG
                     AAVLHMHTLGVPVALTALAFVVGLFVIPFAPETKGEVLPQ"
     gene            7302..7595
                     /locus_tag="VHV99_28140"
     CDS             7302..7595
                     /locus_tag="VHV99_28140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX3639284.1"
                     /translation="MNVLTYSEARAGFKQAMDDVCRDHTPMLITRQGGENVVMVSLED
                     FNAMQETLYLLSSSKNAQRLARSIAQLNAGGATARELLTDEQREKPQRGGRKG"
     gene            7606..7857
                     /locus_tag="VHV99_28145"
     CDS             7606..7857
                     /locus_tag="VHV99_28145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEX3639285.1"
                     /translation="MFTDDAWHDYLYWQETDGKVLRKINTLLEECRRDPYRGTGKPEP
                     LVGSLSGFWSRRITLADRLVYLPRDGKIYVITCRFHYDD"
     gene            complement(8067..>8438)
                     /locus_tag="VHV99_28150"
     CDS             complement(8067..>8438)
                     /locus_tag="VHV99_28150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX3639286.1"
                     /translation="HLLTLAQCAVGVVCLPFIAPLTAVHIGPMQWFWLVGMGVLHTGL
                     AYVLIYGALPKLTTPIIAVLLFVYPLTAIVVDAVVYGRALSVPQLAGMTLIVIASLGV
                     NLGWPLLSMLRSGGRARHHAD"
BASE COUNT         1444 a         2638 c         2793 g         1563 t
ORIGIN      
        1 gcgtctgacc acggcatagg gcgcgcgctc gaaagtcgcg cgaacccatg ccggttcgcc
       61 ctcaaactgc cgcaaacgct gcaggcgcaa caggtcatgc ggccgccagc gcgcatcgcg
      121 cgacaacgcg tgatcggctg caaacggcgg cgctgcgcag acctgcatta caccgcatcc
      181 cattgttcgg cgagacggcg ccgcacttcg atcgacgccg cgcgattctt ttgagccgca
      241 gctgattcca gacgatgcga cagatcgggt ttgccatcgc gtgcgcattg tatctgcccg
      301 gtcaacacct cacgcacgtg ctcgatctgc gcggcatccg gcgcatcggc gtcgacgcct
      361 tcgatcaact gatcgagcag gccgagtttg gcgaacgacg tcatcgagta cgacatcggc
      421 acaatggttt cgccgagcgc gtcgagtgct tcgacggtgc ggcgcgtgac gcgtgcggcc
      481 gcttccttgc ccatggcgtg gaccatcgtg ccgggcgcgt cgagtgcgac gatccggttg
      541 gcctgatagc cgtgcgcgag gaacgcaccc gacatggcgg gtccgacgat tagcgcgatc
      601 accgggtggc cggcgtcgcg tgccgacgca taggcatcga ccgcggcagc gcaggcgagg
      661 tggatgccga gcatttcctc gcgatagccg tacgcctggc ttttgacatc gacgatcgcg
      721 atgatcgggc ggcgcgtacc gctgctggcg tcctgcgcaa tggtctcgcg tacagcgcgc
      781 gcgagctgcc agccttgttc gagaccgacc acattatcgg tcgcgcgcgg aaagcggtta
      841 tcgaggtcgg ggacgaccgc aatgaagcgc gcggtctcgc cgccgagcgg cgcgtcgccg
      901 ctccatacgg gtgccttgcc ggatggctca ccggcgaggg catggaacca gcacgcgccg
      961 cggctgagag tggtgttgtt catgcctgct ccttgccgga catttgcggg ttgtaccccg
     1021 ggttgtacac atcacgcatt gcttccggcg tgacgctggc cgggtcgatt cgcgcgagcc
     1081 ggccaaggaa cgtgtcgacc tgttcgctgc gatgcgcagc cggcacgccc tgcgtgaatg
     1141 ctgagcgcac ggcggcgcgc accgcgttgg catcgtcgtc gaccagttga tcggcgagcg
     1201 cggttgcggc ccgttgttcg ccgccgatca attgccacac gcggcggcga tcgctcgcgt
     1261 cgagctcttc gatgccggct tcctgttcga tcacctcggg gccgttcatg ccgagccgcc
     1321 cttgctttgt cacgacgaga tacgagcaca gcgccgccgc cagcgacatg ccgccgaaac
     1381 agccgaccat ccccgcaatc acgccgacca ccggcacatg ccggcgcagc gcgacgatcg
     1441 cggcctgcat ctcggcgatc accgcgaggc cgagattggc ttcctgaagc ctgacgccgc
     1501 cggtttcgaa cagcacgacg gggcgcacga gcttgccgcg ctcgcagtcg tgcaaggcca
     1561 tttcgagcgc ggcggcgatc ttgctgcccg acacttcgcc gatgctgccg ccctggaacg
     1621 ctgactcgat cgcggcgacc acggccggct cgccgtcaat ggtgccgcgc gcgatcacgc
     1681 agccgtcatc cgcctggcag acgacacctt gcagcggcag ccacggcgat tcgatcttgt
     1741 cgaacggtcc gagcagttcg cggaacgtgc ccgcgtcgag cagcgagcgg gcgcgttcgc
     1801 gcgccggcag ttcgatgaag ctttcgcgca gcattggcgc gggacgtgca gtgtcgacgg
     1861 tgttcatgcg tcgtctccct gttccgctgc ttcgatggct tccgcgagcc gcagcgccac
     1921 gacgccgggt gtcgcgccga agtcgttgat ttcgatttgc gccgcgccgt cgtagcgcgt
     1981 gaagaagcgg tcgagcacgc tcttccagat gtggctgtag ccgtcgacgc tggtacgtac
     2041 gagcacatgc gcggccagcg cgccagcgtt atccgagggc gacagcagca cttccagatc
     2101 gcccgaaccg accacgccga catgcgcgcg ggtcgtgaca gcgcgttgcg ccggatagtc
     2161 gaaggtcaga tgttccatga ggctgaactc ccgccggcgt cgtgatgcgc cagcatgtcg
     2221 agaaagaggg tggcggcgag cagatcggct gcgccgccag gggatgcgtt aagcgacagc
     2281 aattcgtgtt cgagtgcggc cagcgcggca tggccgtccc gcgtcgagct cccgccggct
     2341 tgcagcacgc ggcgcgcgcc gcgttgcgcg gcgtgcaagc caggcaggcc cgcgcgatgc
     2401 agcaggcagg tgtcgtcgag cgaggccatg atcgagagta aggcgtcgat gcgcgcggcg
     2461 ttttcgtcga cgccgccggc tcgcgccgcg agcaacgcgg gcaaaccgat atcgatgacg
     2521 tgcgggaaac cgtcttgtgc ctcacggcgc gctccgccta cctgatagcg ctgccgcgcg
     2581 cgctcgccat gactatcggc gggcgcggcg aagcgatccg ggaagcaggc aatttgcgct
     2641 gcgtgcgtgc agatgtgcga cgcgttcaga ggcgcggcgg cgcctggcat agagacgctt
     2701 cgcatcgagg caccggccac cagcaagcag acgatccaga tcgcgccgcg atgtgcgttg
     2761 ctgccgccgg tggcatgcaa catggcctgc tcgccggcgc ggccgatctg cgcgagttcg
     2821 gcgcgcaaca gcgcggatgg ctcgccacgg cggcgcgcgc tgtgcgcgag cgcggcgaag
     2881 gtcggttcga gcgcatgggc cgagcgcagc atggtggcga gatcgaggtc gcgatgcgca
     2941 ccgctgccgc gccggtcgac cagcgcgggt ttcggcgtga gttgcgcttc ttcgatcagc
     3001 gcgttgaccg cgtaacaggc gagctggacg tcggacaggc ccggctgtgc cgcgaccgca
     3061 gcgccgacgg cacgcacgag caaagcttcg cgcgagccca tcgtcaccag ctcctgaagc
     3121 gcgccggcgg ctcgtacagt ccgcccgacc acgtcaccag gtcgtcgatg ctgcgtgccg
     3181 ccagcagcga gcgcttggct tcgccacgtc gaatgccgag atcttccgga tacgcgacaa
     3241 tgccgcgccg gcgcaactcg gccgtcttct ccggcctggc gcgcagcccg atcggcgtca
     3301 cgccggccac cgctgccaac gccgcacgcc gctcgtcgat gccttccgct ttatgcaaat
     3361 acgcaatgcc ttcctcggtg acgacatggc tcacgtcgtc gccgtagatc atcaccggcg
     3421 cgatcggcat gccgctcttc gcgccgacgg caactgcatc gagttcatcg acgaaggtcg
     3481 actcgccgcc tttcttgtag gtctcggcca tctgcacgac cagcttgtgg ccacgcgaga
     3541 ccgggccctg atccttcagc agcttgagcc atgcttcgct cgaatgacgc cggccgcgcg
     3601 gatcgtgacc catgttcggt gcaccgccga aaccggcgag gcggccgcgc gtaaccgttg
     3661 acgaattcgc gtccgcgtcg atctgcaagg tcgaaccgat aaacagatcg acgccgtatt
     3721 gacccgctag ctggcacagc acgcggttcg agcgcaggct gccgtcattg cccgtgaaga
     3781 atacgtcggg gcgcgcctcg atatacgcct ccatgccgac ttcactgccg aagcaatgaa
     3841 tgctgtcgac ccagcccgat tcgatcgcgg ggatcatggt gggatgcgga ttgagcgtcc
     3901 agttgcggca gatcttgcct ttcaggccga gcgattcgcc gtaagtgggc agcagtaatt
     3961 cgatcgccgc ggtatcgaag ccgataccgt gattcagcga gctcacgcca tacggttcgt
     4021 agatgccgcg aatcaccatc atcgcggtga gcacctgcaa gtcgccgata tgccgtgggt
     4081 cgcgcgtgaa cagaggttcg acggcgaagg gcgtgtccgc ctgcacgact acgtccaccc
     4141 acgaacccgg aatatcgacg cgcggcaatt catcgacgat ctcattgacc tgcacgatca
     4201 cgatgccatg ccggaacgcg gcggcctcgg cgatggtggg ggtgtcttcg gtgttcgcgc
     4261 cggtgtacag attgccgtga cggtcggctt tttccgcgca cagcagcgcg acgtgcggcg
     4321 tcagatcgac gaacattcgc gcgtacaact caacgtatgt atagatcgcg ccaatttcca
     4381 gctggccgtc ttcgagcaac tgcgcgaccc gcaaactctg cggcccggca aacgagaaat
     4441 cgaccttgtg cgcgatgccg cgctcgaaca gcgtcagatg ctcgggacgg ccgatgctcg
     4501 aaatcagcag atgcacgtcg tgaatctttt gcggatcgac cctcgcgagc gaacgggaca
     4561 gaaagtcggc ctgtttctga ttgtcgcctt cgagcgcgac ccggtcgccc ggttggatca
     4621 gcgtttcgag cgcatcgacg atacggttcg tcggcaggat gccgtcttcc agccacggcg
     4681 cgatggccgc gaggcgacgg ttcttctcgt cgcgacgcgt ggtccaggaa cgggcggtgg
     4741 gggatgcttc ggcgggtcgg ttcatcggcg gattcagctc ccagtggtgg tgcgcgtgcg
     4801 tctggcgcgc ggcttgggtt gattcgcgag cgcggcttcc gcctgcgcgc gttcggtcag
     4861 gaaacgatgg agcaggtcgc cggtgccgag cagatgcgtg acgaccagag actgtgcagc
     4921 aggaatatca cggcgttgta ctgcgtcgag caaggccgcg tgttcattgt ccgattcgcc
     4981 cttgtacgac ggcaagccga acttcaggcg cagataccgt tcaccacgcc gatgcagtgt
     5041 gccgatcata tcctcgagct gcgggcgggc ggcgggcgca tacaggctca tatgaaacgc
     5101 cacattgcgc gcgacgtaca acgacggatc cgcctcgcgc tcggcggcgc ggcagaggcg
     5161 ggcgcattcg cgcagcgtgg cggcggtgtg aaccggaatc gcgaggccga tggcgagact
     5221 ctccagcgcg gagcgaatct cgtagatttc gcgcgcctcg tccgccgaca agggcgcgac
     5281 ggtcgcacct ttatgcacgg cggctctcgc ccagccctcg ctttcgagct ggcgcaaggc
     5341 ttcgcgcaca ggaattgcgc tgacggagaa atgccgtgcg attgcgtcct gccgcaacgg
     5401 cgcacccggc gccagcgtac cgtcgacaat cgccgcgcgc agcgcctccg caatcacgcg
     5461 ggacgtgctc tcacgcggcg gcgccgcggg taaaagcggg ctgcgcgccg gggcgtccag
     5521 gggaaaacca tctattgcgt cgctcataag atcaaatatt atatataaaa atgcgcgtgt
     5581 tacagcgggg caaacccgga aatcttcagc accggcggcc tgcggtgtcc attcaacgtc
     5641 atccgaggca catcggttca cccagaagcg cgccatctcg tcgttgcctt tgtcaggcaa
     5701 gtaagccagg tacatcaacc gtagggccgg aggagcatga tgaattcact aaccgaccgc
     5761 acgccggtcg cgcggcgccg cacgccgctc aatcgttcgc agatcgcagg gttttgggga
     5821 gcgtgggctg gctggacgct cgacgggatg gactcgttca tttacgcact cgtgttgacg
     5881 cccgcgttga ccgagctgtt gccgcggtcc ggctatgcgg caacgcccgc gaatgtgggc
     5941 ctcgccggtt cgattctgtt cgcgctgttt ctggtcgggt gggggctgtc gtttatctgg
     6001 gggccgttgg cggaccgctt cggccgcacc aaggtgttgg cgggcaccat tttcacgttc
     6061 gccattttca cggggcttgc cgcgacctcg cacaacgtgt gggagctcgc catctatcgc
     6121 ttcattgccg gggtcggcat tgggggtgag tgggcgttgg cggggacgta cgtcgctgaa
     6181 gcgtggccgg aagatcgccg caaaatgggc gccggttatc tgcaaacggg ctactacgcc
     6241 ggcttctttc tggctgcggc gctcaactac acgatcggcg tgcattacgg ctggcgcgcg
     6301 atgttcctga ccggcgcggt gccggtcgtg gtcgcgattc tggtgctgct gcgggtgaag
     6361 gaatcggaaa agtgggagaa ggcggaagcg ggcaccgtgc gcgccaaacc gctgcgcgaa
     6421 atccttgggc cgatgtatcg gcgtcgcacc tgggtcgcct gcattctgct gacgatcgcg
     6481 atcatcggtt tgtgggcagg cgcggtgtac gagccatcgg cggtgatcca gctcgcgacg
     6541 aaggccggta tggcgaagaa cgatgcgatc aggacggcgt cgctggccac cggtttgttg
     6601 tcgattgcga cgattctcgg ttgtcttgcg ctgccgccgc tggcggaacg cttcggccgc
     6661 aaaaagacgc tggcgattta ctttatcggt atggcggtgt cgatcgtggg cagtttcggc
     6721 tgggcgtttt atctaccgaa cggtctcgcg ccgtttattg cgtggctctt tgtgctgggc
     6781 ttctttggcg gcaatttcgc gctgttcagt ttgtggctgc cggagcagtt cgagacccgc
     6841 gtgcgggcga cggcgtttgc gttctgcacg tcgttcgggc ggtttgtcgg tgccggtgtc
     6901 aactttctgc tgggcgcggc cgtgttgcat atgcatacgc tcggggtgcc ggtggcgttg
     6961 acggcgctcg ccttcgtcgt cggtttgttc gtgattccat ttgcgccgga gaccaagggg
     7021 gaggtgttgc cgcagtgaga ttggccgttt ggctgagtgg cgggcgcggg cggcggcagg
     7081 catgctttct atcggttaga tgacgtgttc ctccctcgtt ttgggtttgt cggtttgtac
     7141 ggtaatctgt acaatgattg ccgttgcgta gatgtgtttc ttcgtctgtg ccaccggctc
     7201 acccgccacc cccgcagtgc aaggagtcaa ttcggcggtg ggatcgtctc taacttttga
     7261 caggagcgac tggagggaaa cctctcagat cgataaaacg aatgaacgtc cttacttaca
     7321 gcgaggcgcg tgccggcttt aagcaagcga tggacgacgt ctgccgcgat cacacgccca
     7381 tgctgataac ccggcagggc ggcgaaaatg tggtcatggt ttcgctcgag gatttcaacg
     7441 cgatgcagga gaccttgtat ctgttgagtt cgtcgaagaa tgcccagcgg ctcgccagat
     7501 ctatcgctca attgaacgcc ggcggtgcga ctgcgcgtga actgctgacc gatgaacaaa
     7561 gagagaagcc gcaacgagga ggccgcaaag gctgatagtg tttttatgtt caccgacgac
     7621 gcgtggcacg actacttgta ttggcaagaa actgacggga aagtcttacg caagatcaac
     7681 acgttgctag aggagtgtcg gcgcgatcct tatcgcggga ctggcaaacc ggaaccgtta
     7741 gttgggagct tgagcgggtt ctggtcgcga cgtatcacgc ttgctgatcg cctggtctac
     7801 ttgccgagag acggaaagat ctacgtgatt acttgccgtt tccactacga cgactgaaac
     7861 agaaaaccgg gcggagtcag ttcgattccc gcccggtttt ttcgtttaac gcgtcaacgc
     7921 ataaaacgtt ttgagccctt acctatgcca tcttgttgac accgtcagcg tgtgctgcgt
     7981 gccgaccggc ggcgcgaaag gcaggtggca gatggcaggc ggcttgcagc agccgctccg
     8041 ccacgcgcgg ccgtcacgcc tgactctcaa tcagcgtgat gccgcgcccg tccgcctgaa
     8101 cgcaacatcg aaagcagcgg ccagccgaga ttgaccccga ggctcgcgat cacgatcaat
     8161 gtcatgcccg cgagctgcgg caccgaaagc gcccgcccat acacgacggc atccaccacg
     8221 atcgccgtca gcggatagac gaacagcaaa acggcgatga tcggcgtggt cagtttcggc
     8281 agcgcgccgt agatcagcac atacgccagc cccgtgtgca gcacgcccat gccgaccagc
     8341 cagaaccact gcatcggccc gatatgcacg gcagtgagcg gcgcaatgaa cggcaggcac
     8401 accacgccga ccgcgcattg cgcgagcgtc agcagatg
//
LOCUS       DATASH010001061         3025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2161001,
            whole genome shotgun sequence.
ACCESSION   DATASH010001061 DATASH010000000
VERSION     DATASH010001061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2161001"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..2294)
                     /locus_tag="VHV99_28155"
     CDS             complement(147..2294)
                     /locus_tag="VHV99_28155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007585990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639287.1"
                     /translation="MTAASVSATAFAETVLNAKNSWIGNTFGFGDGTWTQINITAIAV
                     APDGKVYTNAPWDESGAEASVYQNGKMLGFAGGTHGWGNGGGSAIAVNRKYAFVAIAV
                     GNEKGHLVEPGVWPEKGKQWFGISRRQIADPKRAAPFQPAAKSADPHAQLAAGFLMMN
                     DVPTGTSAEVGGLAANDTTLYAANTARDRIEVYDAESMQQKTTWTVHEPGRIALAPDG
                     TLWVLSGTRTDKAPQVEHYTAAGKRIAETFELDAGTIAVDIAVDAQGRVLIADNGPRQ
                     QVLFFSKRNGRYAQSGSLGERGGIFSGTPGKPGPQRFNGLTGVGLDARGNVYVSTNGI
                     GPRYDPIGAGLGATLESYAPDGKQNWQVQGLLFVDGAWIDPSRPDSVYTGNKRFELDL
                     SKPAGQDWKYAGFLSNRFRYPDDPVFHTDQYPGLPIARKLKGHTFLYLTDMYADHLKI
                     YRFDPQHDGETAIPSGFIAGRERAVAKVPNAPPGGDWIWRDANGDGKFDTDEFTLNTS
                     GTKLAGGWGWWVDSAGDIWRTRDNKGIYRFRFGGLDAKGNPIYSYSNLTQYPVPQPFT
                     ELHRAIYEPDTDTMYVTGYTPDTPVDRGFWKEVGRVLVRYDKWSSGHPVQRYAITLPW
                     TTQSKPIATIIGLTVEGQYIFGAEPVGAVHVWDKDSGRELGVIRPGPEVGRASGWVDV
                     PNGISAAKRSNGEYLVFVEEDARGKVMMYRWKP"
     gene            2221..2511
                     /locus_tag="VHV99_28160"
     CDS             2221..2511
                     /locus_tag="VHV99_28160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639288.1"
                     /translation="MPIHEFFAFNTVSANAVALTDAAVIPAAQAHTAIQVLNVKRTKV
                     IPPIRAVPTHARSGTQSPRLTLRGKNAATKIENNSKIFGALSTVKENDLKIG"
     gene            2825..>3025
                     /locus_tag="VHV99_28165"
     CDS             2825..>3025
                     /locus_tag="VHV99_28165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX3639289.1"
                     /translation="MTASALPLAPSHARRIVQLDGLRAIAVLAVFAQHALKAPLWMGV
                     DLFFVLSGFLITGILLERKARQQ"
BASE COUNT          556 a          986 c          860 g          623 t
ORIGIN      
        1 gcttgatata cgacggcacg gtcacgagcg agacgaaagt cggcagcacc agcccgaaga
       61 agttgatcgc tacgttcttg agaatgcctt tgtccatcgg atgccttggt ttaaatcacg
      121 aataaatctt cacgacgccc acgtcatcaa ggcttccagc gatacatcat cactttcccg
      181 cgtgcgtctt cttcaacgaa cacgagatac tcgccattgc tgcgcttcgc ggcgctgatg
      241 ccgttgggca cgtcgaccca gcccgacgcg cggcccactt ccgggcctgg acggatcacg
      301 ccgagctcgc ggccgctgtc cttgtcccat acatgcaccg cgccgaccgg ctcggcgccg
      361 aagatgtatt gcccttcgac cgtgaggccg atgatcgtgg cgatgggttt gctctgtgtg
      421 gtccacggca gcgtgatcgc atagcgctgc accggatggc cgctcgacca tttgtcgtag
      481 cgcaccagca cgcggcccac ctctttccag aagccgcgat cgaccggtgt atcgggcgta
      541 tagccggtca cgtacatcgt gtcggtgtcg ggctcgtaga tcgcgcgatg cagttcggtg
      601 aacggctgcg gcaccgggta ttgcgtcagg ttcgaatacg agtagatggg attgcccttc
      661 gcatccagac cgccgaagcg gaatcgatag atgcctttgt tgtcccgcgt gcgccagata
      721 tcgcccgcgc tatccaccca ccagccccag ccgcccgcga gcttcgtgcc gctcgtgttg
      781 agcgtgaact cgtcggtgtc gaacttgccg tcgccgttcg catcgcgcca gatccagtcg
      841 ccgcctggcg gcgcattcgg caccttggcc accgcgcgtt cacgtccagc gatgaagccc
      901 gacggaatcg ccgtttcgcc gtcgtgctgc gggtcgaagc gatagatctt cagatggtcc
      961 gcgtacatgt ccgtcaggta caggaacgtg tggcccttga gcttgcgggc gatcggcaag
     1021 cccggatact ggtcggtgtg aaacacagga tcatcgggat acctgaagcg gttcgacaga
     1081 aaacccgcgt atttccagtc ctgacccgcg ggcttcgaaa gatcgagttc gaagcgtttg
     1141 ttgccggtat agacgctatc cggacgtgac ggatcgatcc atgcaccgtc gacgaacaac
     1201 aagccttgca cctgccagtt ctgtttgccg tcgggcgcat aactttcgag cgtggcgccg
     1261 agacctgcgc cgatcgggtc gtagcgcgga ccgatgccgt tggtcgacac atagacgttg
     1321 ccgcgcgcat ccaggcccac gccggtcaaa ccgttgaagc gctgcgggcc cggcttgccg
     1381 ggcgtgccgc tgaaaatgcc gccgcgctcg ccgagcgagc ccgactgcgc gtaacggcca
     1441 ttgcgcttgc tgaagaacag cacctgctga cgcgggccgt tgtcggcgat cagaacgcgt
     1501 ccttgtgcgt cgacagcgat atccaccgcg atcgtcccgg cgtccaactc gaacgtctcc
     1561 gcgatgcgct tgcccgcggc ggtgtagtgc tccacctgcg gggccttgtc ggtgcgcgtg
     1621 ccgctcaaca cccagagcgt gccgtcgggg gccagcgcaa tgcggcccgg ttcgtgcacg
     1681 gtccacgtgg ttttctgctg catcgattcc gcgtcataga cttcgatgcg atcgcgcgcc
     1741 gtattcgccg cgtaaagcgt ggtgtcgttc gccgccaaac cgccgacttc ggcgctggtg
     1801 ccggtcggca cgtcgttcat catcagaaag ccggcggcca gttgcgcatg cgggtcagcg
     1861 cttttcgcgg ccggctggaa cggcgcggcg cgcttcggat cggcgatctg ccgacgcgaa
     1921 atgccgaacc attgcttgcc cttctccggc catacgcccg gctcgaccag atgtcccttc
     1981 tcgttgccga ccgcgatcgc cacgaaggcg tacttacgat tcaccgcgat cgcgctgccg
     2041 ccgccgttgc cccagccatg cgtgccgccc gcgaagccga gcatcttgcc gttctggtag
     2101 acgctcgctt cggcgccgct ttcgtcccac ggcgcgttcg tatagacctt gccgtccggc
     2161 gccacggcaa tcgcggtgat gttgatctgc gtccacgtgc cgtcgccgaa gccgaaggta
     2221 ttgccgatcc atgagttctt cgcgttcaac acagtttccg cgaatgccgt agcgctgacc
     2281 gatgccgccg tcattccagc cgcacaggcg catacagcaa tccaggtttt gaacgtgaaa
     2341 cggaccaaag tcattcctcc aatcagggcc gtgccgacgc acgcgcgctc cggcacccaa
     2401 tctccacggc tcacattacg cggaaaaaat gccgcgacga aaatcgaaaa taattcaaaa
     2461 atattcggcg ctttaagcac ggtgaaagaa aatgatttaa aaatcggtta aatggaaatg
     2521 ggcatgaaca cgctcgtccc gcaccgcctt tcgcccatcc ttaccgatgc ggcacgaagc
     2581 cgtccgcact tcttacgcat tacgacacct tcaggacaca agcataaaaa tccatttcaa
     2641 atgggtaacg aaacaccctg ttgcaatctg attcatttat ccaattcatt cccgtttttt
     2701 catcaatttc ttttccctag aatcgaatgc acttccattg cgacgcgaag cgagcgcggc
     2761 cgtgttctca ggacacccgc gcgatccttc gcccggctca tccgcttcac accatggacg
     2821 gttcatgact gctagcgcac tgcccttagc gccttctcac gctcgccgca tcgtgcagct
     2881 cgacggcctg cgcgccatcg ccgtgcttgc cgtattcgcg caacacgcac tgaaagcgcc
     2941 gctgtggatg ggcgtcgatc tgttttttgt tttgagcggc tttctgatta ccggcatcct
     3001 gctcgaacgc aaggcacgcc agcag
//
LOCUS       DATASH010001062         5449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_183348,
            whole genome shotgun sequence.
ACCESSION   DATASH010001062 DATASH010000000
VERSION     DATASH010001062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5449
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183348"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VHV99_28170"
     CDS             <1..267
                     /locus_tag="VHV99_28170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEX3639290.1"
                     /translation="LMLTLINTAYYGVTQFFPGVMVILFSLRVKPAAIATGILTGQIL
                     AIILYIFHVDFGGFNLGLLCLVVNIAVTAALNYGGKRKAVHALS"
     gene            310..1971
                     /locus_tag="VHV99_28175"
     CDS             310..1971
                     /locus_tag="VHV99_28175"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX3639291.1"
                     /translation="MNSIERGPITVFVARKILTMNPAQPHATHVAVRDGRILCVGNLE
                     EISAWGEAEIVDTFQDKVLMPGLVEGHCHLMEGAMWDAVYLGYYDRRGPDGKLWRGLR
                     TLDEVIERLTEAQRAMTDATAPLLAWGFDPIFFGANRLSVRELDTVSTSRPIVVLHAS
                     VHLMNVNSAMLALAGIDENTDIDGIDKDKDGQPTGELQEFAAMFPVYQVIGSQLSIAA
                     SEKTEAIWNFGKVAQLAGVTTATDLVNDLSALGNRNLREVTADPQYPVRIVPAFAPQR
                     SPDNGVEKVLSAICESTEKLAFGPVKFIVDGSIQGFTARLKWPGYVGGQANGLWLIPP
                     SQLVEVFTPFHRAGLQLHIHTNGDEATEVVLNAVEKMLDACPRSDHRHTLQHCQMADA
                     SQLARAAKLGMCINFFSNHIYYWGDAHYAQTMGPDRANRMNAAGSARRLGIPFAFHSD
                     APITQLSPLFTAWCAVQRKTASGRVLGASECIPVADALRAITLGAAYTLKMDHLIGSI
                     EVGKFADFAVLAEDPSEVAPERLKDVEVWGTVLGGRVFQSPAKND"
     gene            1964..2959
                     /locus_tag="VHV99_28180"
     CDS             1964..2959
                     /locus_tag="VHV99_28180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013803836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CobW family GTP-binding protein"
                     /protein_id="HEX3639292.1"
                     /translation="MIDAAGAAAATTTPTSAGGRPAIPLVVLGGYLGAGKTTLLNHVL
                     SNAGQRRVAVLVNDFGDINIDAALIRERTDDVINLENGCVCCSIGGRLVDALVKISAR
                     PARPELLIIEASGVSDPVKIAQIGLLDRAFRLYGMIVAVDAERIGATLLDPYVGDIVR
                     QQIAGATALVLTKTDLVTEQEKAAAMDAVSSLATTSILLESANGSIPLELIFDASVAP
                     RHRTGGQPLAAGGRLNSHQDICSFSFRSDFRLDRKKLRAAFRSLSVPLLRAKGIVWLD
                     QEASPQELHVVGGRILITPFAGRAAAESTIVLIGRFSDEDERTVRRCLEGTQALN"
     gene            3295..3699
                     /locus_tag="VHV99_28185"
     CDS             3295..3699
                     /locus_tag="VHV99_28185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006412544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX3639293.1"
                     /translation="MATTETSASKVTADTLAAFSAAFNRHDAKALMEFMTEDCVFDAA
                     GGSEVHGTRFVGRDAVRAAFEAVFKTFPDAHWGRGRHYVIGECGVSEWVFTGTHAEGW
                     RIEAEGCDLFEFRNGLIAVKRAFRKERPKQNV"
     gene            3719..5134
                     /locus_tag="VHV99_28190"
     CDS             3719..5134
                     /locus_tag="VHV99_28190"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX3639294.1"
                     /translation="MEQSVITRVGEGRSAERDSATQYDPRYDPLVSPGPGHGKQYAPT
                     YWAATAGTPPPDDGPITKDIDVDVAIIGAGYTGLATALCLAREHGIKAVVLEANKTSW
                     GCSSRNGGQGQNASGRLSRSQWIERWGKDIALKMHDEIQEGFDNFKSLVAEIDCDPQP
                     GGHFLIAHRPRMMAKLAAEAKVWKDVFGYPSELIDQATFRREYVNDCEAAGALHEPEG
                     IGIHALKLAYGYLRLAREAGARVHPSSPVIGWETVNGIHHLRTPGGIVRARAVGIATG
                     AYTAPDLHPSLTSRVMPILSNSMVTRPLTQQEIAACNFRTTQVLTDTRTLRFYYRFLP
                     DNRLQIGSRSAITGADAPNPRHYELLIEGMGRKFPALRGVAIDYSWWGWVDVSHDMMP
                     RICQPDPKQSVFYALGYGGNGVSYSQQAGRRLAERIAGKGSKQTLPIFTSPLPGHPFA
                     PFRRIGQRMLYQLYFRRDEKP"
BASE COUNT         1066 a         1635 c         1709 g         1039 t
ORIGIN      
        1 ctgatgctta cgctgatcaa taccgcgtac tacggcgtga cccagttttt cccgggcgtg
       61 atggtcattc tgttttcgct tcgagtcaaa ccggccgcta ttgcaacggg tattctgacc
      121 ggccagatac ttgcgatcat cctttatatt ttccatgtcg atttcggcgg attcaacctg
      181 ggtctgctgt gtctcgtggt caatatcgcg gtgaccgccg cgctgaacta tggtggcaag
      241 cgtaaggcgg tgcacgcgct ctcctagccg tgtagcctgt ggcctaaatc aaagcactct
      301 tacttactta tgaattcgat tgaacgaggt cccatcacag tcttcgtcgc cagaaagatt
      361 ctgacgatga atccggccca gccgcacgcc acgcatgtcg ccgtgcgaga cggcaggatt
      421 ctctgcgtcg gcaatctgga ggagatcagt gcgtggggcg aggctgagat cgtcgacacg
      481 tttcaggaca aagtgttgat gccgggtctc gtcgaagggc attgccatct gatggaaggc
      541 gcgatgtggg atgccgtgta tctcggctac tacgatcggc gcggtccgga cggcaagttg
      601 tggcgaggtc tgagaacgtt ggatgaggtg atcgaacggc tcactgaagc gcaacgcgcc
      661 atgacggacg caaccgcgcc gctgctggcg tggggcttcg atccgatttt cttcggcgca
      721 aaccgcctgt cggtcaggga actcgatacc gtttcgacga gccggcccat tgtcgtgctg
      781 cacgcgagtg tgcatctgat gaacgtcaac agcgcgatgc tggcgcttgc cggtatcgac
      841 gagaacaccg acatcgacgg gatagacaaa gacaaagatg gccagccgac cggcgagttg
      901 caggaattcg ccgcgatgtt cccggtctat caggtgatcg gcagccagct ttccattgcg
      961 gcgagtgaaa agacagaggc catctggaat ttcggcaagg tcgcgcaact ggccggcgtg
     1021 accaccgcga cggatctggt caacgatctc tcggcgctgg gcaatcgcaa tctgcgcgaa
     1081 gtcacggccg acccgcagta tccggtcaga atcgtgcccg cgtttgcgcc gcagcgaagt
     1141 cccgacaacg gtgtcgaaaa agtgctgtcg gcgatttgcg agagcacgga gaaattggcg
     1201 ttcggcccgg tcaaattcat cgtcgatgga tcgattcagg gcttcacggc gcggctgaag
     1261 tggccgggtt atgtcggcgg acaagcgaac ggattgtggc tgattccgcc gagccagttg
     1321 gtggaagtgt tcacgccatt tcatcgcgcc gggctgcaat tgcatattca taccaacggc
     1381 gatgaagcca ccgaagtcgt cttgaacgcc gtcgagaaga tgctcgacgc gtgtccgcgc
     1441 agcgatcacc gtcatacgtt gcagcactgc cagatggccg acgcgagcca gttggcccgc
     1501 gcggcgaaac ttggcatgtg catcaacttt ttctccaacc atatctatta ctggggcgac
     1561 gcccattatg cgcagaccat gggcccggac cgggcgaacc ggatgaatgc cgcgggttct
     1621 gcccgacgtc tggggattcc ctttgcgttc cattcggacg cgccgatcac gcagttgagc
     1681 ccgctgttta ccgcctggtg cgcggtgcag cgcaagaccg catcgggacg cgtgctgggc
     1741 gcgagcgagt gcattcccgt ggccgatgca ttgcgtgcga tcacgctggg cgcggcctat
     1801 acgctcaaga tggatcatct gatcggcagc atcgaggtgg gcaaattcgc ggacttcgcc
     1861 gtgcttgctg aggacccgag cgaggtcgcg cctgaacgtc taaaagacgt cgaagtgtgg
     1921 ggaacggtgt tgggcggtcg cgtgtttcag tcgccggcaa aaaatgattg atgctgctgg
     1981 tgctgctgcg gccacgacca cacccacgtc cgcgggcggg cggccagcca ttccgttggt
     2041 cgtgctcggc ggctatctgg gcgctggcaa gacaaccctg ctcaaccatg tgctctcgaa
     2101 tgcgggtcag cgccgcgtgg ctgtgctggt caacgatttc ggcgatatca atatcgacgc
     2161 cgcgctgatc cgcgaacgca ccgacgatgt catcaatctg gaaaacggct gcgtgtgttg
     2221 ctccatcggc ggccgcctgg tcgacgcgct cgtcaagatc agcgcgcggc ctgcgcgtcc
     2281 cgagttgttg atcatcgaag ccagcggcgt gtccgatcct gtgaagatcg cgcagatcgg
     2341 cttgctcgac cgggcgttca ggttgtacgg aatgatcgtt gccgttgacg cggaacgaat
     2401 tggtgccacg ttgctcgatc catacgtggg cgatatcgtg cgtcaacaga tcgctggcgc
     2461 gacggctctc gtgctcacca agaccgacct ggtcacagag caggaaaagg cggcggcgat
     2521 ggatgcggtg tcgtcgctcg cgaccacttc catcctgctc gaatcggcaa acggatccat
     2581 tccgctggag ctgatcttcg acgcgagtgt cgcgcctcgc catcgaaccg gcgggcagcc
     2641 tctcgcggcg ggtgggcgcc tcaatagcca tcaggatatc tgcagctttt cctttcgctc
     2701 ggacttccgg ctcgacagga agaagcttag ggcggcgttt cgcagtctgt cggttccgct
     2761 attgagagcg aagggcatcg tctggctgga ccaggaggct tctccgcagg aactccatgt
     2821 ggttggcggc cgcattctga ttacgccttt tgcggggcgc gcggcggctg agtcgacgat
     2881 cgtcctgatc ggccgtttca gcgacgaaga cgagcgtacg gtgaggcgtt gccttgaagg
     2941 cacgcaagcg ttgaactgac acctgcctcg cgccagcgtg ccgggctcag tgggctgatg
     3001 gtgccaggcg cgatgagtac accgagtgcg ccgagcacac cgagtgcgcc gagcccatcg
     3061 agcccatcaa tcgcatcaat cgcatcaccc ccacaaagcc atccacggcc tgaacccgaa
     3121 aaaaacctcg gttccagccc agcccgcccg cgcgctcccc acacgcgctg caccaggcga
     3181 atccatcgca ctggccctac cgccggccgc ccgccaaact tacacttttc ctcaagagat
     3241 cgccgctcag gcgacctcac aagcctcacc caattcacga agcaggagac atgcatggcc
     3301 acaacagaaa cgtccgcatc gaaggtgacc gcggacacgc tcgccgcctt ttccgctgcg
     3361 ttcaaccgtc acgacgcgaa ggcgctgatg gaattcatga ccgaagactg cgtgttcgac
     3421 gccgccggcg gaagcgaagt ccacggtacg cgtttcgtcg gtcgcgacgc ggtgcgcgcc
     3481 gccttcgaag cggtcttcaa gacctttccg gatgcgcatt ggggccgtgg ccgtcactac
     3541 gtcatcggcg agtgcggcgt gtccgaatgg gtgttcacgg ggacgcacgc ggaaggctgg
     3601 cgcatcgaag cagaagggtg cgatctcttc gagttccgca atggactgat cgccgtgaag
     3661 cgcgcgttcc gcaaggaacg cccgaagcag aacgtttgac gccgacgagg agcttcacat
     3721 ggagcaaagt gtcatcacac gggtcggcga aggtcggtcc gcagagcggg acagcgcgac
     3781 gcaatacgac ccgagatacg atccgctggt ttcgccaggg cccggccacg gcaagcaata
     3841 cgcccccacc tattgggccg cgaccgccgg cacgccgccg cccgacgatg gcccgatcac
     3901 gaaagacatc gacgtggacg tggcgatcat cggcgcaggc tatacgggac tcgcgacggc
     3961 gctttgtctc gcgcgcgagc atggcatcaa ggccgtggtg ctcgaggcca acaaaaccag
     4021 ttggggttgc agcagccgca atggcggaca agggcagaat gcatcgggac gtctgtcgcg
     4081 ttcgcaatgg atcgagcggt ggggtaaaga catcgcgttg aaaatgcacg acgagatcca
     4141 ggaaggcttc gacaacttca agtcgctggt ggctgaaatc gattgcgatc cgcagccggg
     4201 cggccacttc ctgattgcgc accggccgcg catgatggcc aagctcgccg ccgaagccaa
     4261 agtatggaaa gacgttttcg gttacccgtc cgaactcatc gatcaggcta cgtttcgccg
     4321 cgaatacgtc aacgattgcg aagcagcggg cgcgctccat gaaccggaag gtatcggtat
     4381 tcatgcgttg aaactcgcgt atggctatct gcggctcgcg cgcgaagcgg gcgcgcgcgt
     4441 tcatccgtcg agtccggtga tcgggtggga gaccgtcaac gggattcatc atttgcgcac
     4501 gccgggtggc atcgtgcgtg cgcgcgcggt cggcatcgcg accggcgctt atacggcgcc
     4561 cgacctccat ccttcgctga cgagccgcgt catgccgatc ctgtcgaatt cgatggtgac
     4621 ccgtccgctc acgcagcagg aaatcgctgc ctgcaatttt cgtacgaccc aggtgctcac
     4681 ggacacgcgc accttgcgct tctactatcg tttcctgccg gacaatcggc tgcagatcgg
     4741 cagccgtagc gcgatcaccg gcgccgatgc gccgaacccg cggcattacg agctgcttat
     4801 cgaaggcatg ggccgcaaat tcccggcgtt gcgcggtgtt gcgatcgatt attcatggtg
     4861 gggctgggtg gacgtcagcc acgatatgat gccgcgtatc tgtcagcctg atccgaagca
     4921 gtcggtgttt tacgcactgg gctacggcgg caacggtgtg tcatattcgc agcaggcggg
     4981 ccgccggctc gccgaacgga tcgccggtaa gggatcgaag cagacgttgc cgatctttac
     5041 ttcgccgttg cccggccatc cgttcgcacc gttccgtcgc attggacaac ggatgctcta
     5101 tcagctctat ttcaggcgcg acgaaaagcc gtgaaggcgg cctgcgtacc gcgtcatccg
     5161 cggtacgcag cgcaccattt accgtgcctc gcgcgcacgg caccgcgcag gcaggcaagt
     5221 ccgcgcattc agtcataaga aaaaacaata agcagcgagg cgcgcaagcg tcctcgctcg
     5281 atccgaattg acggcgcccc ggtggcggct gcatccgcag caccgcacga ccggcgtctg
     5341 aataagcagg atatggaggt gacatgcaag tatcagcgaa taaaagcgaa ttgaagaccg
     5401 gcctcaagga gcgccacatg accatgatcg cgctaggcgg cgtgatcgg
//
LOCUS       DATASH010001063         2974 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5724096,
            whole genome shotgun sequence.
ACCESSION   DATASH010001063 DATASH010000000
VERSION     DATASH010001063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5724096"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..119
                     /locus_tag="VHV99_28195"
     CDS             <1..119
                     /locus_tag="VHV99_28195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX3639295.1"
                     /translation="VELAKTFGGADGYKYVNGVLDKLSAQLRAAETQAARKH"
     gene            431..1627
                     /locus_tag="VHV99_28200"
     CDS             431..1627
                     /locus_tag="VHV99_28200"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018434493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX3639296.1"
                     /translation="MNSVTEPLVRLAARVDAIQPFYVMELAKEAALLERDGRDIIHMG
                     IGEPDFTAPEPVIEAAASALRRGVTQYTNALGLHALREAISTHYAEVYGVSVDPARIV
                     VTAGASAALLLACAALVDRDDEVLMPDPCYPCNRHFVIAAEGKPVMVPSGPAERFQLT
                     AADVERLWNERTRGVLLASPSNPTGTSIEPAELERIVKAVRARGGFTIVDEIYQGLSY
                     DAQPVSALSFGEDVITVNSFSKYFNMTGWRLGWLVVPPGMVGAFEKLAQNLFICASAL
                     AQHAALACFEPDTIAIYEARRLEFKRRRDFIAPALESLGFAVPVMPDGAFYVYADCRT
                     VAHRAAGDSAALTKAMLHDAGVVLVPGMDFGTHAPKDYIRLSYATALPKLEEAVERLA
                     KLFGRH"
     gene            complement(1832..>2974)
                     /locus_tag="VHV99_28205"
     CDS             complement(1832..>2974)
                     /locus_tag="VHV99_28205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX3639297.1"
                     /translation="QVVRGALRRGTRMSHLLFSIVGGIAVVLALTLWLMPAWRGPLAA
                     KLMPVISAAVQAGPARLLQGNPLPAFGPPRASADDSLSSNSTSSDAASGANNGSNGVT
                     ASNASYDGAFDGTGTSGMGVAALNGLDPRTMQSVAALARLIPAQRVSADARDDRVLVS
                     SREQDLVASYLARRYRVAQEPVSELVKAAFDTGREVGLDPLLLLSVMAIESGFNPYAE
                     SGVGAQGLMQVMSKVHSDKFQYFGGESAALEPVANIKVGALVLKDCIARGGSLPGGLR
                     LYVGSTSQDDGGYGAKVMAERGRLRDVARGRKVPINAPQAPVLTASNTAAAAAASATN
                     GKRVQVTLEGGHPLSTATPVKAPASEQDDANSNATKHVASASELGA"
BASE COUNT          511 a          995 c          969 g          499 t
ORIGIN      
        1 cggtggagct cgccaagacg tttggcggcg cagacggcta caagtacgtg aacggtgtgc
       61 tggacaagct gtcggcgcaa ctgcgcgctg ctgaaacgca ggcggctcgc aagcattgag
      121 gtggggcgcg tgacccggtc atgcgcctcg cgctaaacgt tgacccgccg ggtgccggcc
      181 tgtgaagtgc ggtgccgctg ggacgccagc cgctggttcg ccagccgctg gtttcagcga
      241 ctgctggttt ccgtggccgc cggatcgccg gccgccggat cgccggccgc cggatcgccg
      301 gccggttgaa ttccaaactg aacaatgcag ccccatattg ctgggcgctt gcaaggacgc
      361 cactgcgcac atgaacccat gcgccagacg ccgtcccgct tttgcttctg aactgaactg
      421 gattgatcgt atgaactccg tgaccgaacc cttggtgcgg cttgcagccc gcgtcgacgc
      481 catccagcct ttctacgtga tggaactggc caaggaagcc gcgctgctcg agcgtgatgg
      541 gcgcgacatc atccacatgg gaatcggcga acccgatttc accgcgcccg agccggtcat
      601 cgaagcggcc gcgagcgccc tgcgccgcgg cgtcacgcaa tacaccaatg cgctcggcct
      661 gcacgcgctg cgcgaggcga tctcgacgca ttacgccgag gtctacggcg tcagcgtcga
      721 tccggcgcgg atcgtcgtca ccgctggggc ttctgccgcg ctgctgctgg cttgcgcggc
      781 actggtggat cgcgatgatg aagtgttgat gcccgacccg tgctacccgt gcaaccgtca
      841 tttcgtaatc gctgccgaag gcaagccggt gatggtacca agcgggccgg ccgaacgctt
      901 tcagctgacc gccgccgatg tggagcgtct gtggaacgag cgcacccgtg gcgtgctgct
      961 ggcgtcgccg tcgaacccaa ccggcacgtc gatcgaaccg gcggaactcg aacgcatcgt
     1021 caaggccgtg cgggcgcgtg gcggctttac gatcgtcgac gagatctacc agggcttgag
     1081 ctacgacgcg cagcccgtgt cggcgctctc gttcggcgag gacgtgatca ccgtcaacag
     1141 cttttcaaag tacttcaaca tgacgggctg gcgtttgggc tggctggtag tgccgcccgg
     1201 catggtcggc gcgttcgaaa agctcgcgca gaatctgttc atctgcgcgt cggcgctcgc
     1261 gcagcatgcg gcgctggcct gcttcgaacc ggacaccatt gccatctacg aagcgcgccg
     1321 cctcgaattc aagcgccgcc gcgatttcat tgcgccagcg ctcgaatcgc tgggctttgc
     1381 ggtgccggtg atgcccgacg gcgcgttcta cgtgtacgcc gattgccgca ccgtcgcgca
     1441 tcgggcggcc ggagatagtg cggcgctaac caaagcgatg ctgcacgacg cgggcgtggt
     1501 gctggtgccg ggcatggact tcggcacgca cgcgccgaag gactacatcc ggctgtcgta
     1561 tgcaaccgcc ctgccgaagc tggaggaagc ggtcgagcgg ttagcgaagc tctttgggcg
     1621 gcactgaccg aaaggcataa gcgcccgcgc agacttgagc tcctaagagg ccgccaaagc
     1681 aggtcgccac aacgtgctga cgcacgcgct tcacggccgc gcgccgagcc gcgtgagcgc
     1741 ccagccccgg ccccaaatga aaaaggacac cgcctggtgt ccttttttat acctgctgcc
     1801 ttcgtgccag gctaaccgcg ttacaccacc ctcaagcgcc caactccgaa gccgatgcca
     1861 catgcttggt cgcattggaa ttggcatcgt cctgttcgga cgctggcgcc ttcaccggcg
     1921 ttgccgtgct cagcggatga ccgccttcga gcgtcacctg cacgcgcttg ccgttagtcg
     1981 ccgatgccgc cgcagccgca gccgtgttcg acgcggtcag aaccggcgcc tgcggtgcgt
     2041 tgatcggcac cttgcgacca cgcgccacat cgcgcaaacg gccacgttcg gccatcacct
     2101 tggcgccgta gccgccgtcg tcctgcgagg tcgagccgac gtagagacgc agaccgcccg
     2161 gcagcgaacc gccgcgtgcg atgcaatcct tcagcaccag cgcgcccacc ttgatgttcg
     2221 caaccggttc aagcgcagcg ctctcgccgc cgaaatactg gaatttgtcc gagtgaacct
     2281 tggacatcac ctgcatcagg ccttgtgcgc ccacgccgct ctcggcgtac gggttgaagc
     2341 ccgattcaat cgccatcacg gatagcagca gaagcgggtc gagaccgacc tcgcggccgg
     2401 tgtcgaacgc ggccttcacg agctcgctga cgggctcctg agcgacgcga tagcggcgcg
     2461 ccaggtacga cgcgaccaga tcctgctcgc ggctcgagac cagcacgcga tcatcgcgtg
     2521 cgtcggcgga aacgcgttgc gccggaatca gccgggccag cgcggcgacg ctctgcatgg
     2581 tgcgcgggtc gagcccgttc agcgccgcca cgcccatacc ggacgtgccg gtgccgtcga
     2641 aggcgccgtc atagctggcg ttgctggcgg tgacgccgtt gctaccgttg ttcgcgccgc
     2701 tagcggcgtc gctactggtc gaattcgagg acagcgaatc gtcagcggac gcgcgaggcg
     2761 gtccgaaagc cggcaagggg ttgccctgca gcaggcgggc aggaccggct tgcacagcag
     2821 cggagatgac cggcatcagc ttggcggcaa gggggccgcg ccaggccggc atcagccaca
     2881 gtgtgagtgc cagcacgaca gcgattccgc cgacgatgct gaacaggaga tgactcattc
     2941 gcgtcccgcg acgcaacgca ccacgcacaa cttg
//
LOCUS       DATASH010001064         5365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_7107084,
            whole genome shotgun sequence.
ACCESSION   DATASH010001064 DATASH010000000
VERSION     DATASH010001064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5365
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7107084"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="VHV99_28210"
     CDS             complement(<1..538)
                     /locus_tag="VHV99_28210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008112829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3639298.1"
                     /translation="MSILVIEDDPKTGDYLKKGLRESGYAVDLARTGTDGLHMALEHA
                     YDLVVLDVMLPGIDGWEIMRALRARRDLPVIFLTARDHVSDRIRGLELGADDYLVKPF
                     SFTELVLRIRTLLRRGVIRESDVFEIADLKLDVLRRKVTREGVEIPLTNKEFMLLHLL
                     VRRQGEALSRTQIASEVWD"
     gene            723..1355
                     /locus_tag="VHV99_28215"
     CDS             723..1355
                     /locus_tag="VHV99_28215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639299.1"
                     /translation="MKIVSANAMRTFVAPALVAASLFAAGSIAQAQSTAPTGVRGTVT
                     AFSGDLLKVHTRDGKDVDVKLSKDTPIRGVALANVNDIKPDSYVGTAAIPQPDGTLKA
                     LEVHVFPASMRGSGDGHRPWDLGANSTMTNGTVGSLVVSNGRTITVKYKDGEKKIVIP
                     QDVPIVALEPGDRSLLVPGAKVVLFAHKEADGSMAANFISAGEHGVTPPM"
     gene            1440..2072
                     /locus_tag="VHV99_28220"
     CDS             1440..2072
                     /locus_tag="VHV99_28220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEX3639300.1"
                     /translation="MPEPQARFVVHPLVVRITHWINAFAMVCMVMSGWAIYNASPFFP
                     IRFPAWATVGGWLGGSIAWHFAAMWLLCANGLLYLAFGIARGHFRRKLLPVYPRDVLH
                     DATLAMQFKLPHDTGKYNAVQRALYLFVLLLGVLLVASGLSIWKPVQLSWLTALFGGF
                     DFARRVHFIAMAGVVGFVVVHLALVLLVPRTLLPMLTGRARLSTHERSKP"
     gene            2069..2842
                     /locus_tag="VHV99_28225"
     CDS             2069..2842
                     /locus_tag="VHV99_28225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX3639301.1"
                     /translation="MSESKRPDSRASRIVLADHKPQIERLQRRLFLRSSLSIGALAML
                     SGCNMQDGDSVDKVLWAMSRWNDRVQGWLFDRNKLAPTYSASEITDPFPFNAFYPEFD
                     APDIDGSTYQLEVSGLVSDKRTWNLDQLRALPQASQITRHICIEGWSAIGQWRGVPFR
                     TFLERVGADLSARYVGFKCADRYYSSLDMATALHPQTQLTLDFRDAPLPAKYGYPLKL
                     RVPTKLGFKNPKHIAAIFVTNTNPGGYWEDQGYNWFSGL"
     gene            complement(2863..3876)
                     /locus_tag="VHV99_28230"
     CDS             complement(2863..3876)
                     /locus_tag="VHV99_28230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006052909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX3639302.1"
                     /translation="MSTRSIEPTSAEFGARNGDPVDGAPGSPAQQRLVEWFDALAPNP
                     GITRSSLEGVNLLRANQPMPRMPVMYEPSIVIVCQGRKRGFLGDQVFQYDAQQYLVLS
                     VPLPFECETEASPEEPFLAISVRVDLTMVAELLMALNETHGATQHEPLGIYSTPLDPA
                     LSNAVQRLMEALASTLDARILAPGVVREICYRVLTGEQGDAIRAALTHQNHFGRIAKA
                     LRRIHADYHGELDVDTLASEAGMSVAVFHAQFKAVTATSPMQYVKTTRLHHARLLMVQ
                     DGLNAGAAAARVGYESASQFSREFKRLFGLSPVDEVKRMRTVYDAPPPRVVKPVARYV
                     TAV"
     gene            4196..5248
                     /locus_tag="VHV99_28235"
     CDS             4196..5248
                     /locus_tag="VHV99_28235"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014973303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEX3639303.1"
                     /translation="MSTTYAYAATDASAPLAPFEIQRRELRAHDVQMEVLFCGVCHSD
                     LHQARNEWKNTVFPVVPGHEVVCRVTAVGPDVTKYKAGDVVGVGCLVDSCRTCASCKE
                     DLEQYCENGFVGTYNGVDRVDGQITYGGYSTQLVVDEAFTLRVPDNLDPAGAAPLLCA
                     GITTYSPLRTWGAGPGKKVGIVGLGGLGHMGVKLARAMGAHVVLFTTSPSKIEDAKRL
                     GAHEVVISKNAEEMGAHENSFDLIVNTVAAPHDLNPFLNLLKRDGTMTLVGAPEHDHP
                     SPQVFNLIMKRRRLAGSLIGGIAETQEMLDFCGEHGITSDVEVIPMQGINEAYERMLK
                     SDVKYRFVVDLDSLRK"
BASE COUNT         1002 a         1600 c         1630 g         1133 t
ORIGIN      
        1 tgtcccacac ctccgaggcg atctgcgtac gcgagagcgc ctcaccctgc cggcgtacca
       61 gcaggtgcag cagcatgaat tccttgttgg tcagaggaat ctcgacacct tcgcgagtca
      121 ccttgcgccg caagacgtcg agcttgaggt cggcgatctc gaacacgtca ctttcgcgga
      181 tcacgccacg gcgcaacagc gtgcggatcc gcaacaccag ctcggtgaac gagaacggtt
      241 tgacgaggta atcgtcggcg ccgagttcca ggccgcgaat gcgatcgctg acgtgatcgc
      301 gcgcggtcag gaaaatgacc ggcaaatcgc gccgcgcccg cagcgcacgc atgatttccc
      361 agccgtcgat gcctggcagc atcacgtcca gcaccacgag gtcgtacgcg tgttcgagcg
      421 ccatgtgcag gccatccgtg ccggtgcgcg cgaggtcgac cgcgtagccg ctttcgcgca
      481 gccccttctt caggtagtcg ccggttttcg ggtcgtcttc gatgacgagg atgctcatga
      541 tgcctggatt cccaccccgt gcgccgcctc gcgcgtgggt catggatatg gacgtggatg
      601 tgccgctcat tctacgtggc cgcgacgcac cgttcatgac gtttttgtca tccgtctgtc
      661 actcgccgga gccgctcgcg cgcctaacct gaccctatcc tccacccacc cagagcagac
      721 tcatgaagat tgtttccgct aatgcgatgc gcaccttcgt cgcccccgcg ctcgtcgcag
      781 cctcgttgtt cgccgcagga tccatcgcac aggcccagag caccgcaccc accggcgtac
      841 gcggtacggt gaccgcgttc tcgggcgacc tgctcaaggt gcacacgcgc gacggcaaag
      901 acgttgacgt gaagctcagc aaggacacgc cgattcgcgg cgtcgcgctc gccaatgtga
      961 acgacatcaa gccggacagc tatgtcggta ccgcagcgat cccgcagccc gatggcacgc
     1021 tgaaggcgct cgaagtgcac gtgttccccg ccagcatgcg cggctccggc gatggtcatc
     1081 gcccgtggga tctcggcgcg aacagcacga tgaccaatgg cacggtcggc tcgctcgtgg
     1141 tcagcaacgg ccgtacgatc accgtcaagt acaaggacgg agaaaagaag atcgtgattc
     1201 cgcaagacgt gcctatcgtc gcgcttgaac cgggtgaccg ctcgctgctg gtgcccggcg
     1261 cgaaggttgt gctgttcgcc cataaggaag ccgacggctc gatggcggcg aatttcattt
     1321 cggcaggcga gcatggtgta acgccgccga tgtaatacgg cgtttcttca gccgtgcgaa
     1381 cagccgcgtc gtttaatgac gcggctttca actttttcct tgtgtcgtca ctccccacca
     1441 tgcccgaacc gcaagcgcgc tttgtcgtcc acccgctcgt cgtacgaatc acgcactgga
     1501 tcaacgcatt tgcgatggtc tgcatggtga tgagcggctg ggcgatctat aacgcttcgc
     1561 ccttctttcc aatcagattc ccagcgtggg cgactgttgg cggctggctg ggcggctcga
     1621 ttgcatggca tttcgcggca atgtggctgt tatgcgcgaa tggcctgcta tatctggcgt
     1681 ttggcatcgc gcgtgggcat ttccggcgca agctattgcc ggtctatccg cgcgatgtct
     1741 tacacgatgc cacattagcc atgcaattca agctgccgca cgacaccggc aaatataacg
     1801 cggtgcagcg cgcgctctat ctgttcgtct tgctccttgg cgtgctgctg gttgcctcag
     1861 gactttcgat ctggaagccg gtgcaattgt cgtggcttac cgcactgttc ggcggcttcg
     1921 atttcgcgcg tcgcgtgcac ttcatagcga tggcgggcgt ggtgggtttt gtcgtcgtgc
     1981 acctcgcgct ggtgctgctg gtgccgcgca cgctgttgcc gatgttgacc ggccgcgccc
     2041 ggctctccac gcacgaaagg agcaagccat gagcgaatca aaacgcccgg actcgcgcgc
     2101 ctcgcgcatt gtcctcgccg accacaaacc gcaaatcgaa cgcctgcagc ggcgcctgtt
     2161 tttgcgctca tcgctttcga tcggcgcgct cgcgatgctg tcgggctgca acatgcagga
     2221 cggcgattcg gtcgacaagg tgttgtgggc catgtcgcgc tggaacgatc gcgtgcaggg
     2281 atggctattc gatcgcaaca agctcgcgcc gacctattcg gcgagcgaaa tcaccgaccc
     2341 gttccccttc aacgcgttct atccggagtt cgacgcaccg gatatcgacg gttcgacgta
     2401 tcagctggaa gtgtccggtt tagtgtccga caagcgcaca tggaatctcg atcaactgcg
     2461 cgccttaccg caggcgtcac agattacgcg ccacatctgc attgaaggat ggagtgcgat
     2521 cggacaatgg cgaggcgtgc cgtttcgcac ttttctcgaa cgtgtcggcg ccgatctgag
     2581 cgcacgctat gtcggcttca aatgcgcgga ccgctattac tcgagcctcg atatggcgac
     2641 ggcgttgcat ccgcaaaccc agctgacact cgatttccgc gacgcaccgc tgccggccaa
     2701 atacggctat ccgctaaagc tgcgcgtacc gaccaaactc ggcttcaaga atccaaagca
     2761 catcgcggcg atcttcgtga ccaacacgaa ccccggtggc tactgggaag accaggggta
     2821 caactggttt agcgggctat aacgcttccc gcaagcgccc gcttataccg cggtgacata
     2881 ccgtgcaacc ggcttgacga cgcgcggtgg cggcgcatcg tacacggtgc gcatgcgttt
     2941 gacttcatcc accgggctca agccgaacag gcgcttgaat tcacggctga attgcgacgc
     3001 gctttcataa ccgacgcgcg ccgcggccgc gcccgcattc aaaccatcct gcaccatcag
     3061 caaacgcgca tgatgcaaac gcgtggtctt cacgtactgc atcggcgagg tcgccgtcac
     3121 ggccttgaat tgcgcatgga aaacggcgac gctcataccc gcttcagagg cgagcgtatc
     3181 gacatcgagt tctccgtgat agtcggcgtg gatgcgcctt agcgctttgg cgatgcggcc
     3241 gaaatgattc tgatgcgtga gcgccgcgcg aatcgcgtcg ccctgctcgc ccgtcaacac
     3301 gcgataacag atttcgcgga ccacgccagg cgcgagaatg cgcgcatcga gcgtcgaggc
     3361 caacgcttcc atcaatcgct gcacggcatt ggacaacgcc ggatcgagcg gcgtcgaata
     3421 gatgccgagc ggttcgtgct gcgtggcgcc gtgggtttcg ttcagtgcca tcagcagctc
     3481 ggccaccatg gtcagatcga cgcgcaccga gatcgcgagg aatggctctt ccgggctcgc
     3541 ctcggtttca cattcgaacg gcagcggcac cgacagcacc aggtattgtt gcgcgtcgta
     3601 ctggaacacc tgatcgccga gaaaaccgcg cttgcgaccc tggcagacga tcacgatgct
     3661 cggctcgtac atcacgggca tacgcggcat cggctgattg gcccgcagca gattgacacc
     3721 ctccaggctc gagcgggtga tgccgggatt gggcgcgagc gcgtcgaacc attcgacgag
     3781 acgctgctgg gcaggactgc cgggggcgcc atcgaccgga tcgccattgc gcgcgccgaa
     3841 ctcggccgag gtaggttcaa tggaacgggt tgacatgacg atatagtgat atttaagaga
     3901 ataatgcttg aaatttatca tcaaacgacc tactgcgctc gtcaccagga gttttaggca
     3961 aaccttcaag atattcaggt attccccgct tcgctcccgg ctcctattat gggaaccctg
     4021 ccggatcatc tttttccgcg ctgcgccggg ctcggttgcc cgttcaccat ttcggtgaat
     4081 acgtcgacca ccgcctgcgc ggtgatcccg gttcacaaca aggtttgacc gcatcggcga
     4141 cacgcgtcgc ccatcgtcgg cgccttgcga catccccttt ttgctggagc tacccatgag
     4201 cacgacttat gcctatgcag cgaccgacgc tagcgcgccg ctcgccccgt tcgaaatcca
     4261 gagacgcgaa ctgcgcgccc acgacgtgca aatggaagtc ctgttttgcg gcgtgtgcca
     4321 ttccgacttg catcaggcac gcaacgaatg gaagaacacg gtttttccgg tggtgccggg
     4381 ccatgaagtt gtctgccgcg taacggcggt cggtccggac gtgacgaagt acaaggcagg
     4441 cgacgtggtg ggcgtgggct gcctcgtcga ctcgtgtcgt acgtgtgcga gctgcaagga
     4501 agatctcgag cagtattgcg aaaacggttt tgtcggcacg tacaacggtg tggaccgggt
     4561 tgacggacag atcacctacg gcggttattc gacgcaactg gtggtggatg aggcattcac
     4621 gctgcgcgtg ccggataatc tcgacccggc gggcgccgcg ccgttgctgt gcgcgggtat
     4681 cacgacgtat tcgccgctgc gcacatgggg cgccggtcct ggcaagaagg tcgggattgt
     4741 cggccttggc ggcctcggtc acatgggcgt gaagttggcc cgcgcgatgg gcgcgcatgt
     4801 cgtgttgttt acgacgtcgc cgtcgaagat tgaagatgcg aagcgacttg gtgcgcatga
     4861 ggttgtcatt tcgaagaatg ccgaagaaat gggagcgcat gaaaacagct tcgatttgat
     4921 tgtgaatacc gtggctgcgc cgcatgattt gaatccgttt ttgaatctgc tcaaacgtga
     4981 tgggacgatg acgctggttg gtgcgccgga gcacgatcat ccgtcgccgc aggtgttcaa
     5041 tctgatcatg aagcgtcgcc gtctggcggg ttcgctgatt ggcggaattg ccgagacgca
     5101 ggaaatgctc gatttttgtg gcgagcacgg gattacttcg gatgttgaag ttattccgat
     5161 gcaggggatt aatgaggcct atgaacggat gcttaagagt gatgtgaagt atcggtttgt
     5221 ggttgatctg gattctttga ggaagtgatt tttttgcctg cgaggtgtct ggtggtttga
     5281 gtgccttgca ggcaagggct ttctttggtt gattgcctgt agcgctgata tttgcttgtt
     5341 tttttgcctt cgcggcgctt atggt
//
LOCUS       DATASH010001065         3279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2557432,
            whole genome shotgun sequence.
ACCESSION   DATASH010001065 DATASH010000000
VERSION     DATASH010001065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2557432"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VHV99_28240"
     CDS             <1..384
                     /locus_tag="VHV99_28240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX3639304.1"
                     /translation="VASRKHVIDFVDMHDLGDMLVESGFEIPVMDQETLTITYKSPES
                     LLADVRRWGAYPFEREAASNALARRLHKALLAALEGRRRADGTIALTFEVIYGHAWKA
                     VPRTTAEGHGIVRLEDIGRGSSRSR"
     gene            697..1170
                     /locus_tag="VHV99_28245"
     CDS             697..1170
                     /locus_tag="VHV99_28245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HEX3639305.1"
                     /translation="MEASDLLADSEPVLKDWTMKRNCSISPRQFVGFYVSLALFSLAI
                     AFMLVLVGAWLVLPFTGIELLAVGIAFAIYARHAVDYERVRLFQNRLVIEQVSAERLT
                     QFEFNPRWVRIEPGATPRDQIKLVSRGQTIMIGQHLAQYRRAQFAGELRMWLTRC"
     gene            1236..2872
                     /gene="coxB"
                     /locus_tag="VHV99_28250"
                     /pseudo
     CDS             1236..2872
                     /gene="coxB"
                     /locus_tag="VHV99_28250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486666.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
     gene            2917..>3279
                     /locus_tag="VHV99_28255"
     CDS             2917..>3279
                     /locus_tag="VHV99_28255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010090602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX3639306.1"
                     /translation="MSSIGHDVTAGHEHVHGDHAHETPHGWRRWLFATNHKDIGTLYL
                     IFSFTMFLSGGVMALMIRAELFEPGLQIMRPEFFNQLTTMHGLIMVFGAIMPAFVGFA
                     NWMVPLQIGASDMAFARMN"
BASE COUNT          656 a          950 c         1053 g          620 t
ORIGIN      
        1 gttgcttcgc gcaagcatgt gatcgacttt gtcgacatgc atgacttggg ggatatgctg
       61 gtcgagagcg gcttcgagat tcccgtgatg gaccaggaaa ccctcactat tacctataag
      121 tcgcccgaat cgctgctcgc cgacgtgcgc cgctggggcg cctatccgtt cgagcgcgag
      181 gccgcctcga atgccctcgc gcgcagactg cacaaggctt tgctggctgc gctcgaaggc
      241 cgccggcgcg ccgacggcac gatcgcgctg accttcgaag tgatctacgg ccacgcgtgg
      301 aaagccgtgc cgcgcaccac ggccgaaggt catggaatcg tgcggctcga ggatatcgga
      361 cgaggctcgt cgaggagtcg gtgaggaatt gatgaagcgg taaagaggac atctgttatg
      421 tctgaaatta ccgcttcaaa aggcgcgcaa aagctgcctc gaaacggcgc gaaggcttgt
      481 cgcacctggc ttgtgagccg attgaggctt gcttgtggat gctatggatc gcgcctataa
      541 tgcgtcagtt tgtcgcccgg agttcccact tttggggcgg caggcccgag gcacaggcta
      601 caacatgaag tgacgcagcg gcgcttgagt ggccgtttca gcggtcgcga acaatggccg
      661 cgggcggcga tcggtgacgg tcggcaggag acagcaatgg aggcatcaga tctgctcgcg
      721 gattcagaac cagttctcaa agactggacg atgaaacgca actgttcgat ttcgccgcgg
      781 caatttgtag gtttctacgt gtcgcttgcg ttgttctcgt tggcaattgc attcatgctg
      841 gtgctggtcg gcgcctggct ggtgttgccg ttcaccggta tcgaattgct ggcggtgggc
      901 atcgcgtttg ccatatatgc gcgtcatgct gtcgattacg agcgcgtccg gctgtttcag
      961 aaccggctcg tgatcgagca ggtcagcgcc gagcggctca cgcagttcga attcaatccg
     1021 cgctgggtgc ggatcgaacc gggcgcaacg ccgcgtgacc agatcaaact ggtctcgcgc
     1081 ggccagacga tcatgatagg gcaacatctc gcgcaatatc ggcgcgcgca gttcgcaggc
     1141 gaactgcgta tgtggctcac acgttgttga cgtgttgaga cgcgcggcgt tcaccggggg
     1201 cacgagacag cctgccagcg gggtcgaggg tttgaatgga aattttgggt aaggaagcta
     1261 tgaaaacaat caagcgagcc ctcatgggcg tgctggcgat gagcggactg cttttcgccg
     1321 gtgcggccct ggcagtgggc gatgttccgg gcggccctgc cgtcaacgag atcaatctcc
     1381 agccgcctgc gaccaaaatc gctgaggagc tcttcagcct ccacatgttc atgctggttc
     1441 tttgcctggt gatcttcgtc ggcgtgttcg gcgtgatgtt ctattcgatc tttgcgcacc
     1501 gcaaatcgaa aggtcacaag gcttcgaatt tccacgaaag caccaccgtc gaaatcatct
     1561 ggacgatcgt gccgttcgtc atcgtcgtgc tgatggcgct cccggccacc aagaccgtcg
     1621 tcgcgatgaa ggacaccacg aacgccgacc tcaccatcaa ggtcacgggc taccagtgga
     1681 agtggggcta cgactacgtg aagggtccgg gcgaaggcat caacttcctg tccacgctgg
     1741 ctacgccgcg tgcggaaacc gatggccgtc agccgatttc cactacgtat ctgcaggaag
     1801 tcgacaatcc gctggtcgtc ccggtcgaca agaaaatccg catcatcacc acggcgaacg
     1861 acgtggttca ctcatggtac gtgccggctt tcggcgtgaa gcaggatgcg attccgggtt
     1921 tcgtgcgcga cacgtggttc aaggctgaca agaccggcac gttccgcggc ttctgtaccg
     1981 aactgtgcgg caaggaacat gcgttcatgc cggtggtggt cgaagtgctg tcggcagacg
     2041 actacgcgaa gtggatcgat acgcagaaga agaaaatggc cgcaggccag gatgatccga
     2101 acaagaccta cacgatggcc gagttgatgg agcgcggcgg caaggtgtac gcgtcgaact
     2161 gcgcggtctg ccaccagccg accggcaagg gcgcgggcgc attcccgggc ttgacggcag
     2221 caagatcgcc aacggtccga tcgcggagca cgtgagtctg gtgctgaagg gcaagaatgc
     2281 aatgccttcg tgggcgccga cgctgaacga cgtcgaaatc gcttcggtca tcacgtacga
     2341 acgtaactcg tggggcaatc acaccggcga cattctccag ccgacgcaag tgctggctgc
     2401 gcgcaacggc aagctgccgg aaggcggcaa ccatctggcc gacgcgggtg cggcggccag
     2461 cggcgcgcaa gcggcttcgg gcgcagcggc agcttcgggc gcctcgggcg cggaagcggc
     2521 cgcgagcggt tcggacagcg cggcagcatc gcaagcagca ctgccggcaa gcatttactt
     2581 cgacactggc aagagcacgc tgccggccga cgcaaaagcg gcggtcgacg cggccgtggc
     2641 ctacgcgaaa gcgcatccgg acgccaggtt cacgctgtcg ggcttcaccg acgccaccgg
     2701 ttctgccgaa gtcaacgcga agctcgcaaa gacgcgcgcc gaagccgtgc gcgacgcgct
     2761 gaaagcagcc ggcatcgccg aagaccatat tattttaaag aagccggaaa cgatcacggg
     2821 cggcagcgac gcgaaagaag cacgacgcgt tcagatcagc ccggccgcct gacgtgttca
     2881 tggtcagcag cgcagtattc gcattaggag attgtcatgt ctagcatcgg acacgatgta
     2941 accgcaggcc acgagcacgt gcacggcgac catgcccacg agacgccgca tggctggcgt
     3001 cgttggctgt tcgccaccaa tcacaaggat atcggtacgc tgtatctgat cttctcgttc
     3061 accatgttcc tctccggggg cgtgatggcg ctgatgatcc gtgccgaact gttcgagccg
     3121 ggtctgcaga tcatgcgccc cgagttcttc aaccagttga ccaccatgca cggcctgatc
     3181 atggtgttcg gcgcgatcat gccggccttc gtcggcttcg cgaactggat ggtgccgctg
     3241 cagatcggcg catcggacat ggctttcgcg cggatgaac
//
LOCUS       DATASH010001066         2525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1372810,
            whole genome shotgun sequence.
ACCESSION   DATASH010001066 DATASH010000000
VERSION     DATASH010001066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1372810"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..1298
                     /gene="serS"
                     /locus_tag="VHV99_28260"
     CDS             3..1298
                     /gene="serS"
                     /locus_tag="VHV99_28260"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013338512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX3639307.1"
                     /translation="MLDIQLLRKDLDGVAKRLADRGYTFDAAAFTALEAERRDTQTRT
                     EEMQARRNSLSKQIGAMKGKGEDTSAVMAEVGGIGDEMKASAAKLDDIQKRLSDLLLG
                     VPNVAHESVPVGTDEAGNVEVRRWGTPRQFDFEVKDHVDVGAPLGLDFETGAKLSGAR
                     FTMLRGQIARLHRALAQFMIDTHTQHHGYTEIYTPYIVNPEILYGTGQLPKFADDMFR
                     VEKGGDENTVTQYLISTSEISLTNTVRDSILEADALPVKLTAHSPCFRSEAGSYGRDT
                     RGLIRQHQFDKVEMVQIVAPEASYDALEEMVGHAETILQKLELPYRVITLCTGDMGFS
                     AAKTYDLEVWVPAQNTYREISSCSNTESFQARRMQARFRNAQGKPELVHTLNGSGLAV
                     GRTLVAVLENFQNADGSVTVPAALRPYLGGVERLEVPAA"
     gene            1470..1559
                     /locus_tag="VHV99_28265"
     tRNA            1470..1559
                     /locus_tag="VHV99_28265"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1504..1506,aa:Ser,seq:cga)
     gene            1922..2525
                     /locus_tag="VHV99_28270"
                     /pseudo
     CDS             1922..2525
                     /locus_tag="VHV99_28270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241516.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
BASE COUNT          511 a          710 c          796 g          508 t
ORIGIN      
        1 ccatgctcga cattcagctg ctgcgcaaag acctcgacgg cgtcgccaaa cgcctcgccg
       61 accgcggcta taccttcgac gcagcggcgt tcaccgcgct cgaagcggaa cgccgcgaca
      121 cccagacccg taccgaagaa atgcaggcgc gccgcaacag cctgtcgaag cagatcggcg
      181 cgatgaaagg caagggcgag gacacctcgg cggtgatggc cgaagtcggc ggcatcggcg
      241 acgagatgaa ggcgtcggcg gcgaagctcg acgatattca gaagcgtctg tcggatctgc
      301 tgctcggcgt gccgaacgtc gcgcacgaaa gcgtgccggt cggcaccgac gaagccggca
      361 acgtcgaagt gcgccgctgg ggcacgccgc gtcagttcga tttcgaggtg aaggatcacg
      421 tcgatgtcgg cgcgccgctc ggcctcgact tcgagaccgg cgcgaagctc tcgggcgcgc
      481 gctttaccat gctgcgcggc cagattgcgc ggctgcatcg cgcgctcgcg cagttcatga
      541 tcgacacgca cacgcagcat cacggctata cggagatcta cacgccgtac atcgtgaatc
      601 cggaaattct gtacggcacc ggccaactgc ccaaattcgc cgacgacatg ttccgcgtcg
      661 agaagggcgg tgacgaaaac acagtcacgc agtatctgat ttccacatcg gaaatttcgc
      721 tgacgaacac ggtgcgcgac agcatcctcg aagcagacgc gttgcctgtc aaactgaccg
      781 cgcattcgcc gtgcttccgc tcggaagcgg gctcgtatgg ccgggacacg cgcggtctga
      841 tccgtcagca tcagttcgac aaggtcgaga tggtgcagat cgtcgcgccg gaagcgtcgt
      901 acgacgcgct ggaagagatg gtcggccacg ctgagacgat tctgcagaag ctggagttgc
      961 cgtatcgcgt gatcacgttg tgcacgggcg atatgggctt ctcggcggcc aagacatacg
     1021 acctggaggt gtgggtgccg gcgcagaaca cgtatcgcga aatttcgagc tgctcgaaca
     1081 cggagtcgtt ccaggctcgc cgcatgcagg cgcgtttccg caatgcgcaa ggcaagccgg
     1141 agctggtgca tacgctgaac ggatcgggtc tggcggttgg ccgcacgctc gtggcggtgc
     1201 tggagaattt ccagaacgcg gacggatcgg tgacggtgcc ggcggcgctg cgtccttacc
     1261 tcggtggtgt ggaacggctg gaagtgccgg cggcttgatt gatgccgggt tgatgctcgg
     1321 ctgatgtcgt taccgcttgc caggtcgctt taaaaaattc tggcgagggg cttggaaatc
     1381 ggattcagct gttctataat ctttctctcc caagcaacga cccgcttgga tctgacgaag
     1441 cagttacgtt gctgtgagtt ggaagacccg gaaaggtggc agagtggtcg aatgcgctgg
     1501 actcgaaatc cagtgtacct ttaggggtac cgtgagttcg aatctcaccc tttccgccag
     1561 aattgttcaa aaaaccccgt aaagtcaatg acttgcgggg ttttttgttt ttcggctcac
     1621 gaatcgacta tcaaatgcac tatgggcaga ctgcgatcct agtgtcccca tagattgggc
     1681 gcccgcgcat ttccgcttgt ttgtgtcagc agccgagggg gcggtgacgc acaggtgtca
     1741 gtccgccagc cccagtgatt tgtttgggcg caaactaccg gctttgacgt ttggaactca
     1801 tttgcgcaaa acggtgaagc tcgggatcag cttggccgtc cggtcaccgc taatggatat
     1861 ctttgtctcg tcaaacggga atgccgccgc gtactccgtg ttcagcggcg gcttgtgctt
     1921 aaagatacgc gatgagttgc tggcgggctc gtacagtccg ctgcctgcac gtcgcgtgta
     1981 cataccgaag gcgaacggca agctcagacc actcgggatc ccttgtctac gggatcggat
     2041 cgtgcaaagg gccatgctga tggtcatgga gcctatctgg gagagtgact tccattcggc
     2101 ttcatacgga ttcaggccgg cccgtagcgt acaccacgcg atccgcacgg tgaagcttca
     2161 gttgcaggac ggtggtgagc aaagtgtcgc tggacgctgg gttattgagg gcgacctcgc
     2221 cagctacttc gacacagttc atcatcgcct gcttctgaag ggaatccgca agcggatcgc
     2281 cgatcagcgc ttcctggccc tgctctggaa gttcatcaag gcggggtgtg ttgatcgcgg
     2341 cctgtttcgc gcagcaagcg aaggggttcc tcagggcggt gtcatctcgc cgctgttatc
     2401 caacatcatg ctgcaccaat tcgatgtgtg gatggaaaag aactacctga acaagaaagc
     2461 gcgaaaggac cggtgggcgt ggaacttcgc cattctcaag cagcgtccga ttactgtgcg
     2521 agaga
//
LOCUS       DATASH010001067         3209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5130648,
            whole genome shotgun sequence.
ACCESSION   DATASH010001067 DATASH010000000
VERSION     DATASH010001067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5130648"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..796)
                     /locus_tag="VHV99_28275"
                     /pseudo
     CDS             complement(110..796)
                     /locus_tag="VHV99_28275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048182.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
     gene            complement(1042..1677)
                     /locus_tag="VHV99_28280"
     CDS             complement(1042..1677)
                     /locus_tag="VHV99_28280"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3639308.1"
                     /translation="MTPDPNTPDPSTQEPNAQDYFAGLYQRSDDPWLLRERWYERRKR
                     ALTLAALPDEHYCRGYEPGCANGELTVELASRCETLLAADLNPAAVELARRRVAHLQN
                     VRVEQRAMPDDWPDGAFDLIVISEVAYYLDEAQLAGLIVRLGVSLAEGGTLIACHWRR
                     PIEGWSHSGDHVHQELRAKLALPQLSCYQDDDVVLDVWSSNAESVHQREAH"
     gene            complement(1674..2450)
                     /locus_tag="VHV99_28285"
     CDS             complement(1674..2450)
                     /locus_tag="VHV99_28285"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008351964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX3639309.1"
                     /translation="METVVTHSANDRAIEGNGTPEATWQKSGRLSSLPEVDSAILVPP
                     GARAVIVSPHPDDEILGTGGLLAQLSDLGRKVLIIAVTDGTASHPDSPEWPTVRLAAT
                     RSQETRDALQRLRMKHVALERLHLPDGGGETFESELAEALKARLEPDDIVFGTWRFDG
                     HPDHESVGRAVTAVAGALDLPFVEVPVWTWHWAIPDDTRVPWSRARRIVLDAATLARK
                     VNAIQAFRSQIEADNSTGRAPILPDHVLERLTRPYEVVLI"
     gene            complement(2438..>3209)
                     /locus_tag="VHV99_28290"
     CDS             complement(2438..>3209)
                     /locus_tag="VHV99_28290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016343998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX3639310.1"
                     /translation="HVPESGQAWGVWAAEPPTARVSASAASNTNSVTLHGTKAWCSGA
                     ASITHALVTAWNERDEPILAAVDLHQPGVRITRDGWMAVGMSASASVDVHFDHAKATR
                     IGAPHAYLERAGFWHGGGGIAACWFGAAAAIGEFVKRDTARRADPYKLAHLGAIDTAL
                     SGTASLLREAAARIDREPQSDAMPDAFRVRLAAERSAVDVVERAGRALGAAPLCRNRH
                     FASLMADLPVFIRQSHAERDEAALAERLTNGECGTWKL"
BASE COUNT          593 a          985 c         1010 g          621 t
ORIGIN      
        1 atgcgcgaag accgcaaggc cgtgatcggc ggcggcgtca gcgtcctgcg cgcggtgttc
       61 gacctgctcg acatcgagga aatgcatgtc gctcagggcg cgcctgcgcg atcaggcgtt
      121 ccatggcgtc ggcggcagcc atgtcggcgt cgatagcagg ctgggtggtg acttcaagcg
      181 tcgggttaaa gagtaagccc gcttcgaaat acgccagccg gtatcccatt gcccgctggg
      241 agatgctgta gtgcgccagc gatccgccaa tgaatgcaat ccgcttgcgc ccgagcgcaa
      301 acagatgttg catggcaagt gcggcgcccc gcgcgttgtc gacgttgacg gagcggaaac
      361 ccggcgcgcg caaatcgatc agtacgaccg gccggttcat ggagcgcagg tgagcaagca
      421 actcaggctc gataaatcct gcaaccgcta cggcgtccgg tgcgtggagg cgcatctgct
      481 gggcgagatc atgcgtcggg ccggccgtga gtacagatgg caccagccgc cgctcgcggc
      541 aggcttcttc gaaaccgtgc agaacgtggg aaaaaaacgg gctgactgcg aaattattgt
      601 gctggcgatg aagcaggaac gtgacgcgcc ggattcgggt gcgaagctgg gcagcgtcgt
      661 agccaagttg ccgcgccgct tcgaccacgc gttcacgcgt cgtttccgac aagcccggct
      721 ggtttttaag cgctcgcgat accgtgccga ttgacacact ggctgcgcgc gcgacatcgc
      781 gaatggttgt ggccatcggg tttctcgtct ccgcattgcg tggatttctt gtttatcggc
      841 gctgaggcgt tggtcgcacg cgatgtgttt acctagcggt atcaagttcg caatttggac
      901 gattgtatag taaaacagtc ccaaattcgc ccgatgaatc ttagagaaaa cccgtaagcc
      961 gtttgttgat aaggatttca gttaccttta ttgtttagta aatgtggcgg gaaaattcaa
     1021 cttaacacgt gtcggcaagc cctagtgcgc ttcccgttga tggaccgatt ccgcattcga
     1081 tgaccatacg tcaagcacca cgtcgtcgtc ctggtagcag gacaattgcg gcagcgcgag
     1141 ttttgcacga agttcttgat gtacatgatc acccgaatgt gaccatccct cgatcggccg
     1201 ccgccaatgg caggcgatca gtgttccgcc ttcggcgagc gaaaccccaa gcctcacgat
     1261 gagcccggcg agttgagctt catccaggta atacgccacc tcgctgatta cgatcaggtc
     1321 gaacgcgcca tccggccagt cgtcaggcat ggcgcgctgc tcgacccgaa cattctgaag
     1381 atgcgcgact cgccgccgcg cgagttcgac cgcagccgga ttcaaatccg ccgcgagcag
     1441 cgtctcgcat cgagaagcca gttcaactgt cagttctccg ttcgcgcaac cgggttcata
     1501 tccgcgacag taatgttcgt cgggcagggc ggccagcgtc aacgcgcgct tgcgccgctc
     1561 gtaccagcgt tcgcgaagta gccaggggtc gtcgctgcgt tgataaagac cggcgaaata
     1621 gtcctgcgcg tttggttcct gcgtgctcgg gtccggtgtg ttcgggtccg gtgtcatatc
     1681 agcacgacct cgtagggccg ggtgagacgc tcgagcacat gatcgggcag aatgggtgcg
     1741 cgacccgtcg agttgtcggc ttcgatctgg ctccgaaacg cctggatcgc gttaaccttg
     1801 cgggcaagcg tggccgcatc cagcacgatg cgccgcgctc ggctccaggg cacgcgggta
     1861 tcgtcgggta tcgcccaatg ccatgtccac acggggactt ccacgaacgg caggtcaagc
     1921 gcaccggcca cggcggtcac ggcccggccg accgactcgt ggtcgggatg gccatcgaaa
     1981 cgccaggttc caaatactat gtcgtccggt tcgagacgcg ctttcagcgc ttccgccagt
     2041 tccgactcga acgtttctcc accgccgtca ggaaggtgca gtcgctccag cgcgacatgc
     2101 ttcatgcgca gacgttgtag cgcgtcgcgc gtttcttgcg agcgagtcgc ggcgagcctc
     2161 accgtgggcc attccggcga gtcaggatgg ctcgccgtgc cgtccgttac ggcgatgatc
     2221 aacaccttgc gacccagatc ggacaattga gcgagcaggc ctcccgtgcc caggatttca
     2281 tcgtccggat gcggcgaaac aatcacggcg cgtgcgccgg gtggcacgag gatcgccgaa
     2341 tcgacttcgg gcaggctcga gagtcgcccg gatttttgcc aggttgcttc cggcgtgcca
     2401 tttccctcga tggcgcgatc gtttgccgag tgagttacta cagtttccat gtgccgcact
     2461 ccccattggt aagtcgttca gcaagcgcgg cttcatcgcg ttcggcatga ctctgccgga
     2521 taaaaacagg tagatcggcc atcaggctgg cgaaatgacg gttacggcaa agaggcgcgg
     2581 cgcccaacgc acgaccggcg cgctcgacga cgtcaaccgc agagcgttcc gccgccagcc
     2641 ggacccgaaa ggcatccggc attgcatcgg actgcggctc gcggtcaatc cgtgccgccg
     2701 cttcgcgcag caggctggcc gtgccggaga gcgccgtgtc aatagcgccc agatgcgcga
     2761 gcttgtacgg atcggcgcgg cgtgccgtat cgcgtttgac gaattccccg attgccgccg
     2821 ccgccccgaa ccagcatgca gcgatcccgc cgccgccgtg ccagaaaccc gctcgctcga
     2881 gataggcgtg cggcgcgccg atgcgggtag ctttggcgtg atcgaaatgg acgtctacgc
     2941 tcgcgctcgc cgacatgccg acagccatcc agccatcgcg cgttatgcgc acgcccggtt
     3001 gatggagatc gactgcggcg agaattggct cgtcccgttc gttccaggct gtgacgaggg
     3061 catgggtgat cgaggccgct ccagaacacc aagccttcgt gccgtgcaac gtgacgctgt
     3121 ttgtgttgga cgcagcgctc gccgagacgc gagccgtggg cggttctgct gcccaaacgc
     3181 cccacgcctg accgctttcc gggacgtgt
//
LOCUS       DATASH010001068         6393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1768728,
            whole genome shotgun sequence.
ACCESSION   DATASH010001068 DATASH010000000
VERSION     DATASH010001068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6393
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1768728"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /locus_tag="VHV99_28295"
     CDS             complement(<1..992)
                     /locus_tag="VHV99_28295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018438830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HEX3639311.1"
                     /translation="MTRKDINSTGTDNGARRGNLVTGLVYVFEQVMPDPFVLAIGLTL
                     MVALLAVVFAPHASLPVILASWYGGTFNILGFALQMILILATGYAIADAPLVQRGLRA
                     MAAGVRTPTRAALLVFPIVAVAAWLNWGLGLVVGALLSREIAKRVKVDFAWLVAGSYS
                     AWSVCNSGLSSSIALSQASHRNALNLVEKATGQVIPLSQTVFAPFVFIPTVLVVVVMT
                     AIFIWMHPKQDDVVAFNETQPELAAGAEADPHARNAQASSFAARAEQSMLGTLLVLAL
                     GVGYLAMTWYTKGFELDINTTILIFLLVGLALQRTPIAYADAIRRAARQTGSMLL"
     gene            complement(1139..2497)
                     /locus_tag="VHV99_28300"
     CDS             complement(1139..2497)
                     /locus_tag="VHV99_28300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3639312.1"
                     /translation="MSRSTAVNVQTFINDHPFSPFQWLVFFMCFIIVLLDGFDTAAIG
                     FIAPSLIAEWGIARPALAPVLSAALFGLACGALVSGPLSDRLGRRSMLLGSVLLFGVA
                     CLASAFSNSIEHLTILRFITGVGLGAAMPNAVTMMGEYCPDRRRATVINLMFCGFPLG
                     AAFGGFLAAWMIPHFGWRSVLVLGGITPLLLLILLALKMPESVRYMVANNKPVERIRA
                     ALARISSEAAEGNSFVMTETAPQTGGKGLGVVLSRSYIIGSVMLWIAYFMGLVIFYAS
                     INWMPILLKDAGLTPQRATLISALFPLGGVGAVVCGVLMDRFNANRIIAACYALTAVS
                     VYFIGQAVGNVGALVFIVFVAGVLMNTAQSSLPALAAAFYPTQGRGTGVAWMLGIGRF
                     GGIAGSFLVAELTRRHFTFAGIFATVAVAGLISCVALLIKQAARPHVADTAATKAESF
                     GH"
     gene            complement(2652..3734)
                     /locus_tag="VHV99_28305"
     CDS             complement(2652..3734)
                     /locus_tag="VHV99_28305"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012405758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HEX3639313.1"
                     /translation="MSKKYRIAVIPGDGIGVEVMPEAIRVLDAVKQRFGIELEYQHIE
                     WASCDYYAKHGKMMPDDWKAQLQSADAILFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YINLRPARLFAGVPSPLAGRKAGDIDFWIVRENTEGEYSSVGGVMFEGTEREFVLQES
                     VFTRRGSERVLKFAFDLAQRRERKKITVATKSNGIAISMPWWDKVAASIAAQYPDVTW
                     DKQHIDILCARFVLNPDRFDVVVATNLFGDILSDLGPACTGTIGLAPSGNLNPDRKFP
                     SLFEPVHGSAPDIAGKNIANPIAMIWSAAMMLDFLGNQEGKEREAHDAILAAIEATLV
                     EGPHTGDLGGKANTTEVGQAIAAKLA"
     gene            complement(3847..5850)
                     /gene="prpR"
                     /locus_tag="VHV99_28310"
     CDS             complement(3847..5850)
                     /gene="prpR"
                     /locus_tag="VHV99_28310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HEX3639314.1"
                     /translation="MSTPPFDPAQRPRIWAVSISRLRDLFFDIAGEYVERADLRIVSH
                     GFEDAVHEVDVVGAGRPDVVIAGGSNGAYLKTRVGVPVVLINPTGFDVMHALARARRD
                     GAKVALVTHGDTPDEVRRFTAAYGIDVTLASYQSAQDAESVVLDLRDRGIDVIVGPGL
                     VTDLAANAGMGAVFLYSRASVRAAFDTALEVAQATRRETVRRQRLDNLLQHLRDGVVA
                     LDAQGRVEAMNQRLASVLGIDAAHAVGRALLDLAPDLAGSLPDSDGDTFCTVRGASYV
                     VHRGPLASSGSAAGTVLTFQESRAVERLDRTLRSRQRVQQFSARYQLGDIVGAADSIE
                     RVRTLVQRYARSDATVLILGESGTGKEMVAQSMHQLSARRDFAFVAMNCGAFPEALLE
                     SELFGYEEGAFTGARKGGKAGLIEVAHRGTLFLDEIAEMPLSLQSRLLRVLQEREVVR
                     LGSTEPTRVDIRVVAATHRALTEGIEAGSFRADLYYRLNILSIALPPLRERPTDLLPL
                     AVELLLQAASREPRLAARLPDANAAARVLNGLSEPFKRYMWPGNVRELQNVIERIAVE
                     LADVDANTDSKDSSANSSTNPGSALETVFTRDVLRTVAPEIFEQPHARAKKAALTLRE
                     RSRHVEADEIRAALAACGGDRDAVCQALGISKTTLWRKLNAAQ"
     gene            6208..>6393
                     /locus_tag="VHV99_28315"
     CDS             6208..>6393
                     /locus_tag="VHV99_28315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEX3639315.1"
                     /translation="MSEADNSTPNAGAFKPKKSVALSGVTAGNTALCTVGKTGNDLHY
                     RGYDILDIATSCEFEEIA"
BASE COUNT         1248 a         2025 c         2003 g         1117 t
ORIGIN      
        1 agcagcatcg agccggtctg ccgcgcggcg cggcgaatcg cgtcggcata agcgatcggg
       61 gtgcgctgca gcgccagccc gaccagcaga aaaatcagga tcgtggtgtt gatgtccagc
      121 tcgaagccct tggtgtacca cgtcatcgcg agatagccga cacccagcgc caacacgagc
      181 agtgtgccga gcatcgactg ttccgcgcgg gcagcgaagg acgaggcttg cgcgttccgc
      241 gcatgcggat cggcctcggc accggcggca agctcgggct gcgtttcatt gaaagcgacc
      301 acatcgtcct gcttcggatg catccagatg aagatcgccg tcattacgac caccaccagc
      361 acggtcggga tgaagacgaa cggggcgaac acggtctgac tcagcggaat cacctggccg
      421 gtggctttct caacgaggtt taatgcgttg cgatgcgagg cctgcgacaa agcgatcgaa
      481 ctggacaaac cgctattgca caccgaccac gccgagtagc tgcctgcgac gagccatgcg
      541 aaatccacct tcacgcgctt ggcgatttcg cgcgacagca gggcgccgac cacgagacca
      601 agtccccagt tgagccatgc ggccactgcg acgatcggga agaccagcag cgcggcgcgg
      661 gtcggcgtgc gcacgccggc agccatcgcc cgcaggccgc gctgaacgag cggtgcgtcg
      721 gcgatcgcgt agcccgtcgc gagaatcagg atcatctgca gcgcgaagcc gagaatgttg
      781 aacgtgccac cgtaccagga ggcgaggatg accggcagcg aagcatgcgg cgcgaagacc
      841 accgccagca gggccaccat cagggtcaag ccgattgcaa ggacaaacgg gtccggcatg
      901 acctgctcga acacatagac gagacccgtc acgaggtttc cacggcgcgc gccgttatcg
      961 gtgccggttg agttgatgtc tttcctggtc atgattgctg gtccgttggc cgtacgctgc
     1021 catggccttt cacaattgaa agcccggaca tttccggttg cctggcgtac gagagcgcca
     1081 ttatgccgac gatccaattt actcgtacgc cactcgggca gacgccatca agagccattc
     1141 aatggccaaa cgactccgct ttcgtggcag ctgtgtctgc cacatgcggc cgcgccgcct
     1201 gcttgatcag caacgccacg cacgaaatca gacccgctac cgcgaccgtc gcgaagatac
     1261 ccgcgaacgt gaagtggcgg cgcgtcagtt cagccacaag gaacgagccc gcgatcccac
     1321 cgaagcggcc gatccccagc atccacgcca cgccggtgcc gcgaccctgc gtcggataaa
     1381 acgcggcggc cagtgccggc agcgacgatt gcgcggtatt catcagcacg cccgcgacga
     1441 agacgatgaa caccagcgcg cccacattgc cgaccgcctg gccgatgaag tacacgctca
     1501 cggcggtcag cgcatagcag gccgcaataa tgcggttcgc gttgaagcgg tccatcagca
     1561 caccgcacac cacggcgccc acgccgccga gcgggaacag cgcggaaatc agcgtcgcgc
     1621 gttgcggcgt gaggccggcg tccttcaaga gaatcggcat ccagttgatc gacgcgtaga
     1681 agatcacgag gcccatgaaa taggcgatcc acaacatcac cgaaccgatg atgtaggaac
     1741 gcgacagcac cacgcccaga cctttgccgc cggtttgcgg ggcggtttcg gtcatgacga
     1801 agctgttgcc ctctgccgct tcgctcgaaa tgcgcgccag cgccgcacga atccgttcga
     1861 ccggcttgtt gttggccacc atgtagcgga ccgattccgg catcttcagc gccagcagga
     1921 tcaacagcaa caacggcgtg atgccgccga gcaccagcac gctgcgccag ccgaagtgcg
     1981 gaatcatcca cgcggcgaga aagccgccga aggccgcgcc cagcggaaag ccgcagaaca
     2041 tcaggttgat cacggtcgcg cggcggcgat ccgggcagta ctcgcccatc atcgtcacgg
     2101 cgttcggcat ggccgcaccg agtccgacgc cggtgatgaa gcgcaatatc gtcagatgtt
     2161 cgatgctgtt cgagaacgcc gaggcgaggc aggccacgcc gaacagcagc accgagccga
     2221 gcagcatgga gcgtcgcccg agccggtcgg agagcggccc tgagacgagc gcgccgcacg
     2281 cgaggccgaa cagcgccgcg ctcagcaccg gcgcgagtgc cggtctcgcg atgccccatt
     2341 cggcgatcag cgaaggcgcg atgaagccga ttgcggccgt gtcaaaaccg tccagcagga
     2401 cgatgatgaa acacatgaaa aagacgagcc actggaacgg cgagaaggga tggtcgttga
     2461 taaacgtttg aacgtttaca gcggtgctgc ggctcatgat tgtctcctga ctgcttagta
     2521 ggggaaggct tgccgttacg gctggtcgta cgagccttct ttcttttacg attgtgcgcc
     2581 gattacaagc gctacacgat atctacaccg gcggcgaacc ggctccgcga ccgggacgac
     2641 gccgccgaga ctcaggcgag ttttgccgcg attgcctgac ccacctcggt ggtgttcgcc
     2701 ttgcccccca ggtcgccggt gtgcgggcct tcgaccagcg tcgcttcgat tgccgcgaga
     2761 atcgcgtcgt gcgcctcacg ctccttgcct tcctgattgc cgagaaagtc gagcatcatc
     2821 gcggccgacc agatcatcgc gatcggattg gcgatgttct tgccggcgat atccggcgcc
     2881 gagccgtgca ccggctcgaa cagcgagggg aacttgcggt cgggattcag attgcccgac
     2941 ggcgcgaggc cgatcgtgcc ggtacaggcc gggccgagat cggacaggat gtcgccgaac
     3001 agattggtgg caaccaccac gtcgaagcga tccggattca gcacaaagcg cgcgcagaga
     3061 atgtcgatgt gctgcttgtc ccacgtcacg tcgggatact gcgcggcgat gctcgccgcg
     3121 actttgtccc accacggcat gctgatcgcg atgccgttgc tcttggttgc gacggtgatc
     3181 ttcttgcgct cgcggcgctg cgcgagatcg aatgcgaact tcagcacgcg ctcgctgccg
     3241 cgccgcgtga acaccgattc ctgcagcacg aattcgcgct ccgtgccttc gaacatcacg
     3301 ccgcccaccg acgaatattc gccctcggtg ttttcacgca cgatccagaa atcgatgtcg
     3361 cccgctttgc ggcctgcgag cggtgaaggc acgccggcaa aaagccgggc cgggcgcagg
     3421 ttgatgtact gatcgaattc gcggcggaac ttcagcagcg agccccacag cgagatgtga
     3481 tcgggcaccg tgtcgggcca gccgaccgcg ccgaacagga tcgcgtcggc ggattgcagt
     3541 tgcgctttcc agtcgtccgg catcatcttg ccgtgtttcg cgtagtagtc gcagcttgcc
     3601 cattcgatgt gctgatactc gagttcgatg ccgaagcgct gtttgaccgc atcgagcacg
     3661 cgaatggctt cgggcatcac ttcgacaccg ataccgtcgc cgggtatgac cgcaattcga
     3721 tatttcttcg acatatttct gctctccatc ggggctgata gacggttcga tcgcgcttat
     3781 tctatgcctg ctgtttaagg cgcgttgcgg ttgtgaagac ggcgaaagag ggtgacgtgg
     3841 ttcaggctac tgcgccgcgt tcagcttccg ccataaggtc gttttgctga tgccgagcgc
     3901 ctggcaaacc gcgtcgcgat cgccgccgca tgcggcgagc gcggcgcgga tttcgtctgc
     3961 ttccacgtga cggctgcgct cgcgtagcgt gagcgccgct ttcttcgcgc gtgcgtgggg
     4021 ctgctcgaag atttcaggtg caacggtgcg cagtacgtct cgcgtaaaga cggtttccaa
     4081 tgcgctgcct gggttcgtgc tcgagttcgc gcttgaatcc tttgagtccg tgttcgcgtc
     4141 tacgtctgcc agttcaaccg caatccgttc gatcacgttc tgcaattcgc gcacgttgcc
     4201 cggccacatg taacgcttga acggttcgct gagaccgtta aggacgcgtg ccgcggcatt
     4261 cgcatcgggt agccgggcag ccagcctcgg ctcacgcgat gccgcctgca aaagcagttc
     4321 gaccgcgagc ggcagcagat cggttggccg ctcgcgtagc ggcggcagtg cgatgctcag
     4381 aatgttgagc cggtagtaaa ggtccgcacg gaaactgccg gcctcgatgc cctcggtcaa
     4441 cgcacgatgc gtcgcggcga ccacccggat gtccacgcgc gtcggctccg tcgaaccgag
     4501 ccgtacgact tcacgttcct gcagtacgcg caaaagccgg ctctgcaacg acaacggcat
     4561 ctcggcgatt tcgtcgagaa acagcgtgcc gcggtgcgcc acttcgatca gccccgcctt
     4621 gccgcctttg cgtgcgccgg tgaacgcgcc ttcctcatag ccgaacaatt cgctttcgag
     4681 caacgcctca ggaaacgccc cgcaattcat ggcgacgaag gcgaagtcgc gccgcgcgct
     4741 caactgatgc atgctctgcg caaccatctc cttgccggtg ccgctttcgc cgagaatcag
     4801 caccgtggcg tccgatctgg cgtaacgctg cacgagcgtg cgaacccgtt cgatcgaatc
     4861 ggccgcgccg acgatatcgc cgagttgata acgcgcactg aactgctgca cacgctgtcg
     4921 tgaacgcagc gtgcggtcga gccgttcgac ggcacgcgat tcctgaaacg tcagcaccgt
     4981 gcctgccgcc gagccgctgc tcgcgagcgg cccgcgatgc acgacatagc tcgcgccacg
     5041 taccgtgcag aacgtgtcgc cgtccgaatc cggcaagctg ccagcgagat cgggcgcgag
     5101 gtcgagcagc gcgcggccca ctgcatgggc ggcgtcgatg ccgagcacgc tggcaaggcg
     5161 ctggttcatc gcttcgacgc gcccttgcgc atcgagtgcg acgacgccgt cgcgcagatg
     5221 ctgcagcaga ttgtcgagcc gttgacggcg caccgtttcg cggcgggtgg cctgcgcgac
     5281 ttcgagtgcg gtatcgaacg cggcacgtac cgatgcgcgt gaatagagaa acaccgcgcc
     5341 catgccggcg tttgccgcaa gatcggtgac gagacctggg ccgactatga cgtcgatgcc
     5401 tcgatcgcgc agatcgagca cgacgctttc ggcatcttgc gcggactggt acgaagcgag
     5461 cgtcacgtcg atgccgtacg cagcggtgaa gcggcgcact tcgtcgggcg tgtcgccgtg
     5521 cgtgacgagc gcgaccttcg cgccgtcgcg ccgcgctcgc gcgagcgcgt gcatgacgtc
     5581 gaaaccggtt gggttgatca gcacgaccgg gacgccgacg cgcgtcttca gatacgcgcc
     5641 gttggagccg ccggcgatga cgacatccgg acgcccggcg ccgaccacgt cgacttcatg
     5701 cacggcatcc tcgaaaccat gcgagacgat gcgcagatcg gcgcgttcga cgtattcgcc
     5761 ggcgatgtcg aagaacagat cgcgcagtcg gctaatgctg acggcccaga tacgagggcg
     5821 ctgcgcgggg tcgaaggggg gcgtgctcac gatggaggct cgttaatggg cttaaagcgg
     5881 ggcttagagc ggggcttcga agcggccgta gaagcgggct cagaagcgtg ctcagaagcg
     5941 tgaagggcag caaggctgaa aagctcatcc attatgccca cgtgctttcc ggatttgaaa
     6001 ctcgtaattt cacttatgaa atcgggcggc ggagtggccg ggatatgatt ttttgttaaa
     6061 tcagtcactt agcgcttcat gtgccgcctg gcccattctt tgcattatca gcctccgttt
     6121 tcaccggtca ccgcagtgaa acgctgaaag acaagctcgg cgcgctgaat gccgcacgaa
     6181 cgcaaccccg cagttggaga cgaaatcatg agtgaagccg ataacagcac gccgaacgca
     6241 ggcgccttca aaccgaagaa atccgtggcc ctgtccggcg tgacggctgg caacactgct
     6301 ttgtgcacgg tcggcaagac cggcaacgat ctgcattacc gcggctacga cattctcgat
     6361 atcgcgacct catgcgaatt cgaagagatc gcg
//
LOCUS       DATASH010001069         1838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2952725,
            whole genome shotgun sequence.
ACCESSION   DATASH010001069 DATASH010000000
VERSION     DATASH010001069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2952725"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VHV99_28320"
     CDS             <1..331
                     /locus_tag="VHV99_28320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX3639316.1"
                     /translation="LEGPQDCVAEIRDTYKDRRDVLVPGLNKLGWPVELPRATMFAWA
                     KIPERYRALGSVEFSKKLLLEAKVAVSPGVGFGEHGDGHVRFSLIENEERTRQALRGI
                     KQMFKAG"
     gene            399..>1838
                     /locus_tag="VHV99_28325"
     CDS             399..>1838
                     /locus_tag="VHV99_28325"
                     /inference="COORDINATES: protein motif:HMM:NF026221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HEX3639317.1"
                     /translation="MRFSSLTRREFLQSTTVTGAAAALVPATLGRSWGQATPGVVEQV
                     PAWASKPMRWAQLTLVEDDPAHFDLDFWLNYFKRTRSDGVCLSGGGCVAYYPTKVPFH
                     HRSVWLGDRDVLGELISGCRKLDMSVLVRTDPHATYDDVKTAHPDWIAVDAEGNPRRH
                     WSAPEMWVTCAYGPYNFEFMTAVHGEIMSRYHVDGIFLNRWEGSGDCYCIHCREDFKS
                     ATGFDLPRTIDVRDPARRAYLVWRQKKLVSVLDRWNTEIRAINPEASMIPNNGSGAMS
                     PLDSIATSQRAPMLVADRQARHGVAEPWLVGKTAKEYRATMGNKPVIGLFGVGLEEQY
                     RWKDSVNSNAEIRIWVLDAIANGMRPWFSKFSATLHDERWLKGVEEVYVWTEMNQHYL
                     THQQSLARVGLVYSQQTAWYYGGEHAAANVENYALGWYQALIESRIPFDMVHDQLLDS
                     ERIASYKTLILPNIAALSHAQCDQLRAFVH"
BASE COUNT          372 a          527 c          544 g          395 t
ORIGIN      
        1 gctcgaaggt ccacaggact gtgtcgccga gattcgtgac acctacaaag accgccgtga
       61 cgtgctggtg cctggcctga acaaactggg atggccggtt gaactgccca gggcgacgat
      121 gtttgcatgg gcgaagatcc cggaacgata ccgcgctcta ggctcggttg agttttcaaa
      181 gaagctcctg ctggaagcaa aagtcgcagt aagcccaggt gtcggctttg gtgagcatgg
      241 cgatgggcat gtgcgattct ccctgataga gaacgaggag cggacacggc aggccttgcg
      301 tgggatcaag cagatgttca aggccggcta gcgtcgattg ctgcggattc atcggagcgc
      361 tggccgtagg gtgcgttctc tcaagtagta ttacgcgcat gagattttcg tccctgacgc
      421 gccgtgagtt tttgcagtcc actactgtta ccggcgctgc cgccgcattg gttcccgcta
      481 cgctggggcg ttcctggggg caggccacgc caggtgttgt cgagcaagtt cccgcctggg
      541 cttcgaagcc catgcgttgg gcccaactca cactagtcga agacgacccg gcccacttcg
      601 acctcgactt ctggctgaat tacttcaagc gcacgcgatc cgatggagtc tgcctcagcg
      661 gcgggggctg cgtggcctac tatcccacaa aggttccctt tcatcaccgc agcgtgtggc
      721 tcggcgaccg cgatgtgctg ggtgagttga tctccggttg ccgaaagctc gacatgtcgg
      781 tgcttgtgcg taccgatcct cacgctacct atgacgacgt gaagactgcg catcccgact
      841 ggatcgcagt cgatgcagaa ggcaatcctc gccgtcactg gtcggcgccg gagatgtggg
      901 taacatgcgc ctacggccct tataacttcg agtttatgac ggctgtccac ggggagatca
      961 tgtcgcgcta tcacgtcgac ggcatctttc tcaatcgatg ggaaggctct ggcgactgct
     1021 actgtatcca ctgtcgcgag gacttcaaat cggctacagg cttcgatctt cctcggacaa
     1081 tcgacgtgcg ggatcccgct cgccgagcct atctggtgtg gcggcaaaag aaacttgtca
     1141 gcgtgctcga tcgatggaac acggagattc gcgcgatcaa tccggaagcc agcatgattc
     1201 ccaacaacgg cagcggtgcc atgagcccgc ttgattcaat tgcaacaagt cagcgtgccc
     1261 caatgctcgt tgccgatcgg caggctcgcc acggcgtcgc cgagccctgg ctggttggca
     1321 agacggccaa ggaatatcgc gcgaccatgg gcaataagcc cgtcatcggc ctttttggtg
     1381 tgggtctcga agagcaatac cgatggaagg atagcgtcaa cagcaatgca gagattcgca
     1441 tctgggtgct tgatgccatt gccaacggaa tgcgcccgtg gttcagcaaa ttttccgcga
     1501 cactccacga cgagcgttgg ctcaaggggg ttgaagaggt ctatgtctgg acagaaatga
     1561 accagcatta cctcacacat caacagtcgc tcgctcgcgt cggtctagtg tattcgcagc
     1621 agactgcctg gtactacggt ggcgaacacg ccgcagccaa tgtcgagaat tatgcgctgg
     1681 gttggtatca ggcgctgatc gaatcgcgta ttccgttcga tatggtgcac gatcagctgc
     1741 tcgattctga gcgcatcgcc tcctacaaaa ccctcatcct gcccaacatt gctgcgctca
     1801 gccacgccca gtgcgaccag cttcgagcgt ttgtccac
//
LOCUS       DATASH010001070         6449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5725328,
            whole genome shotgun sequence.
ACCESSION   DATASH010001070 DATASH010000000
VERSION     DATASH010001070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6449
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5725328"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="VHV99_28330"
     CDS             complement(<1..781)
                     /locus_tag="VHV99_28330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3639318.1"
                     /translation="MTTLIRGGTIIDAENTYRADILCADPQDGGTILQIGADLEAPAG
                     ATIVDAGGQYVMPGGIDPHTHMELPFMGTTASDDFYTGTAAGLSGGTTSIIDFVIPSP
                     KQSLMEAFKAWRGWAEKASADYGFHVAVTWWDDSVYRDMGTLVHEHGVSSFKHFMAYK
                     NAIMADDEVLVNSFSRSLELGALPTVHAENGELVFQLQRQLLAKGFTGPEAHPLSRPP
                     EVEGEAANRAIRIAQVLGVPVYIVHVSAKDAVDAIARARSEG"
     gene            complement(866..2377)
                     /locus_tag="VHV99_28335"
     CDS             complement(866..2377)
                     /locus_tag="VHV99_28335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family nucleobase:cation symporter-1"
                     /protein_id="HEX3639319.1"
                     /translation="MKQTAQPVDPQFAAGAQGSSLYNDDLAPTGVAQRTWRWYHFAAL
                     WVGMVMNIASYMLAAGLTEEGMSPWQAVATVLLGNLIVLVPMLLIGHAGAKHGIPYAV
                     LVRSSFGTQGAKLPAMLRAIVACGWYGIQTWLGGSAIYTLLNILTGNALHGAALPFLD
                     ISLAQLACFLVFWALQIYFIVHGTDSIRWLESWSAPIKIVMCIALVWWATSKAGGLGS
                     MLSAPSQFVAGGKKKGMFWATFWPGLTAMVGFWATLALNIPDFTRFAKTQRDQIIGQS
                     VGLPIPMALLSVISVVVTSATVVIYGKAIWDPIDLTSRMTGIGVGLALIILTLDTMCC
                     NLAANLVGPAYDFSSLWPKGISYRVGGMITATIAIVMMPWKILATTQGYIFTWLVGYS
                     ALLGPVAGILMVDYFLIRGTRLDPRELFDEHGEYSYTGGWNIGAVVALVIGVLPNLPG
                     FLHTAFPASFPNVPAIFNTLYTYAWFVGLALASIVYSAWMKLSKGPSARVASA"
     gene            complement(2502..3848)
                     /gene="preA"
                     /locus_tag="VHV99_28340"
     CDS             complement(2502..3848)
                     /gene="preA"
                     /locus_tag="VHV99_28340"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HEX3639320.1"
                     /translation="MADLRCTIAGITSPNPFWLASAPPTDKAYNVNRAFEAGWGGVVW
                     KTLGLDPHVVNVSSRYGAVQWNGQRIAGLNNIELITDRPLDVNLKEIAQVKRDWPDRA
                     MIVSLMVPCNERDWKWILPLVEDTGADAVELNFGCPHGMSERGMGAAVGQVPEYVEMV
                     TRWVKEGTKLPCLVKLTPNISDIRLGSRAAYKGGADGVSLINTINSIVAVDLDAMSPL
                     PMVDGKGTHGGYCGPAVKPIALNMVAEIARDVETPNLPISGIGGISTWRDAAEFMVLG
                     AGSVQVCTAAMHYGFRIVSDLADGLSNWMDEKGYATLDDIRGRAVPNVTDWKYLNLKY
                     DIKARIDQDKCIQCGLCHIACEDTAHQAIMKEKDGVRHFEVMDSECVGCNLCMHVCPV
                     EQCITMERVDSGEYANWTTHANNPARVNAGASVTSDAAEATEPAHAHAHAHAAKAA"
     gene            complement(3951..5300)
                     /locus_tag="VHV99_28345"
     CDS             complement(3951..5300)
                     /locus_tag="VHV99_28345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012217800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3639321.1"
                     /translation="MAIKQTGDIAPQRLSADQLSCEFSDIAPLLDASAAAAAASRCHY
                     CYDAPCVNACPTQIDIPSFIRKISNGNLKGAALDILSANPLGGMCARVCPTEILCEGA
                     CVRNHQDAKPVAIGALQRHATDWAMARGEPLFKRAAETGRHVAVVGAGPAGLACAHRL
                     ALAGHDVIVYDAHEKAGGLNEYGIAAYKTVDDFAQREVEWLCSIGGIEIKHGVALGRD
                     VDLDALRKQHDAVFLAIGLTGVRALAMEGEDLRGVMNAVDFIEQVRSARDFGTVPVGR
                     RVVVIGGGNTAVDAAVQSRKLGATSVTMVYRRGVESMSATWAERDFAQTHGVTLITHA
                     TPMRLIGAGGVVTGVEFERTSSDGNQERFVVEADMVLKAIGQTLVPVGVERELLTLDG
                     SRIAVDASGQTSLPGVWAGGDCAATGGIDLTVQAVQDGKVAAAAIDAQFARTAVKAA"
     gene            complement(5444..>6449)
                     /locus_tag="VHV99_28350"
     CDS             complement(5444..>6449)
                     /locus_tag="VHV99_28350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HEX3639322.1"
                     /translation="TGSHADSQPTGGRFDGIYGVLGGLEVIRSLNDHGIETEHPVEVV
                     IWTNEEGSRFAPAMVASGVFAGVFTLDYGLSRKDVDGKTIGEELKRIGYAGDLPCGGR
                     PLHAAFELHIEQGPILEAEQKTIGVVTDAQGQRWYEITLTGQEAHAGPTPMPRRRDAL
                     LGAARVVDLVNRIGLDNAPFGCATVGMMQVYPNSRNVIPGRVFFTVDFRHPDDAVLAK
                     MDAALRQGVADIANGIGLETALEQIFYYAPVAFNEACVKSVRAAAERFGYPHRNMVSG
                     AGHDACYLSQVAPTSMVFVPCVDGISHNEIEDASFEWIEAGANVLLHAMLERACEPVS
                     "
BASE COUNT         1215 a         2136 c         1883 g         1215 t
ORIGIN      
        1 ggccttcgct acgggcgcgc gcaatcgcgt cgaccgcatc tttcgctgac acgtggacga
       61 tgtacaccgg cacgcccagc acctgggcga tgcggatcgc ccgattggcc gcctcgccct
      121 ccacttccgg cggccgcgac aacggatggg cctccggacc cgtaaagccc tttgccagca
      181 actgacgctg cagctgaaac accagttcac cgttttccgc atgcacggta ggcagcgcgc
      241 cgagttcgag tgaacgcgag aagctgttca ccagcacttc gtcatccgcc atgatggcgt
      301 tcttgtaggc catgaagtgc ttgaagctcg atacgccatg ttcgtgcacc agcgtgccca
      361 tgtcgcgata gaccgaatcg tcccaccacg tcaccgcgac gtgaaagcca taatcggctg
      421 aagccttttc ggcccagccg cgccacgcct tgaaggcctc catcaacgat tgcttcggac
      481 tcggaatcac gaagtcgatg atgctggtcg tgccgccgga caaacccgcg gccgttcccg
      541 tatagaaatc gtcgctggcc gtggtgccca tgaacggcaa ttccatatgc gtgtgcggat
      601 cgatgccgcc gggcattacg tactgcccgc ccgcatcgac gatcgtggcg cccgccggcg
      661 cctccaggtc cgcgccgatc tgcaggatcg tgccgccgtc ctgcgggtcc gcgcacaaga
      721 tgtccgcacg atacgtgttc tccgcatcga taatcgtgcc gccgcgaatc agggtcgtca
      781 tgttgccgcc tccttatgaa ctgctggttt cggtgctacg tacttcattc tcttcatggg
      841 cgcgctcatg gatacgctca tacgctcatg cactcgccac gcgcgcgctc ggtcctttac
      901 tcagtttcat ccacgcgctg tatacgatcg aggccagcgc gagtccgacg aaccatgcat
      961 acgtatagag cgtgttgaaa atcgccggca cattcgggaa cgatgccgga aacgcggtat
     1021 gcaaaaagcc cggcagattc ggcagcacgc cgatcacgag cgccaccacc gcgccgatat
     1081 tccagccgcc ggtgtagctg tactcgccat gctcgtcgaa cagttcccgc gggtccagcc
     1141 gggtgccgcg aatcaggaag tagtcgacca tcagaatgcc cgccaccgga ccgagcaatg
     1201 ccgagtaacc gaccagccac gtgaagatat agccctgcgt ggtagcgaga atcttccacg
     1261 gcatcatcac aatggcgatg gtcgcggtaa tcatgccgcc gacgcgatac gaaatgcctt
     1321 tcggccacag gctcgaaaag tcataagccg ggccgacgag attcgccgcg agattgcagc
     1381 acatcgtatc cagcgtcagg atgatcaacg cgaggccgac gccaatgccg gtcatacggc
     1441 tcgtcaggtc gatcggatcc cagatcgcct tgccgtagat caccacggtc gccgacgtca
     1501 ccaccacgga aatcaccgag agcagtgcca tcggaatcgg caaacccacc gactggccga
     1561 tgatctgatc acgctgtgtt ttggcaaagc gtgtgaagtc agggatgttc agcgcgagcg
     1621 ttgcccagaa acccaccatc gccgtgaggc cgggccagaa ggtcgcccag aacatgccct
     1681 tcttcttgcc gcccgccacg aattgcgacg gcgccgacag catcgagccg agtccgcccg
     1741 ctttcgaggt cgcccaccac accagcgcga tacacatcac gatcttgatc ggtgcggacc
     1801 agctttcgag ccagcgaatc gaatcggtgc cgtgcacgat gaagtaaatc tgcagtgccc
     1861 agaacacgag gaaacacgcg agctgcgcaa gcgagatgtc gaggaacggc aaggccgcgc
     1921 cgtgcagcgc gttgccggtc agaatgttca gcagcgtgta gatcgcgctg ccgccaagcc
     1981 acgtttgaat gccgtaccag ccacacgcga cgatcgcccg cagcatcgcc ggcaatttcg
     2041 cgccttgcgt gccgaacgaa gaacgcacca gcaccgcgta gggaatcccg tgcttcgcgc
     2101 ccgcgtgacc gatcagcagc atcggcacca gcacgatcag attgccgagc agcaccgttg
     2161 ccaccgcctg ccacggcgac atgccctcct ccgtcaaacc ggccgcgagc atgtacgacg
     2221 caatgttcat caccatcccg acccagagcg cggcaaagtg ataccacctc cacgtgcgct
     2281 gtgcgacgcc ggtcggcgca aggtcatcgt tgtaaagact gctgccctgc gcgccggccg
     2341 caaactgcgg atcgacgggt tgcgctgtct gcttcatcga aagatctcca ctgatcgttg
     2401 aggcctgcgc ggcttctggt ggcgcgtcag gccttgaggg aacattacag cttcagtaca
     2461 agcgcttcgc ttcgtttcct tttctacagt cagctcgtgt ttcaggcagc ttttgctgcg
     2521 tgcgcatgcg catgcgcatg cgcaggttcg gtggcttcag ccgcgtctga cgtcacgctt
     2581 gctccggcat tcacccgtgc cggattattc gcatgcgtgg tccagttcgc gtattcaccg
     2641 ctgtccaccc gctccatcgt gatgcactgc tcgaccgggc acacgtgcat gcacagatta
     2701 cagccgacac attcggagtc catcacttca aaatgccgaa cgccatcttt ttctttcatg
     2761 atcgcctgat gagcggtgtc ttcgcaggcg atatggcaca ggccgcactg aatgcacttg
     2821 tcctgatcga tgcgcgcctt gatgtcgtat ttgaggttca ggtacttcca gtcggtgacg
     2881 ttcggcacgg cgcggccgcg aatgtcgtcg agcgtcgcgt agcctttttc gtccatccag
     2941 ttcgagaggc cgtccgcgag gtcggagacg atacggaatc catagtgcat cgccgccgtg
     3001 cagacctgta cgctgcccgc gcccagcacc atgaattcgg ccgcatcgcg ccacgtcgaa
     3061 ataccgccaa tgccggagat cggtagattc ggcgtttcca cgtcgcgcgc aatttcagcc
     3121 accatgttca gcgcgatcgg ctttaccgcc ggaccgcaat agccgccgtg cgtgcccttg
     3181 ccgtcgacca tcggcaacgg cgacatggcg tcgagatcca ccgcgacgat cgagttgatg
     3241 gtgttgatca gcgaaacgcc gtccgcgccg cctttatagg cggcacgcga accgaggcga
     3301 atgtcgctga tattcggcgt gagcttgacg aggcacggca gcttcgtgcc ttccttgacc
     3361 cagcgcgtga ccatttccac atactcgggc acttgcccga cagcggcacc catgccacgc
     3421 tcgctcatgc cgtgcgggca gccgaaattc agttcgaccg catcggcgcc ggtgtcttcg
     3481 acgagcggca agatccactt ccagtcgcgt tcgttgcacg gcaccatcag cgagacgatc
     3541 atcgcgcggt ccggccagtc gcgtttgacc tgggcgattt ccttaagatt cacgtcgagc
     3601 ggacggtcgg tgatcagttc gatgttgttc aggcccgcga tacgctggcc gttccattgc
     3661 accgcgccat agcgcgagct gacgttcact acatgcgggt cgaggcccag cgtcttccac
     3721 acgaccccgc cccagcccgc ctcgaatgcg cggttcacgt tataggcttt gtcggtcggc
     3781 ggcgcggagg cgagccagaa aggattcggc gaggtaatgc cggcaatcgt acagcgcaga
     3841 tcggccatgt tcggctcctt ggggactgct gttttatcct ggtgtgatgc gtggcttaag
     3901 tggcttaagt ggcttgagtg gcttaagcgg tgtcggcggc ttcagcggtc tcacgcggct
     3961 ttgacggcgg tgcgcgcgaa ctgagcatca atcgcggcgg cggcaacctt gccgtcctgc
     4021 accgcttgta ccgtcagatc gatcccgcct gtcgccgcgc agtcgccccc cgcccatacg
     4081 cctggcagcg aggtttgtcc gctcgcatcg accgcgatgc ggctaccgtc gagcgtcaac
     4141 agttcgcgct cgacgcccac gggcaccagc gtctgcccga ttgccttgag caccatgtcg
     4201 gcttcgacca cgaagcgctc ctgattgcca tcgctcgacg tgcgttcgaa ttcgacacct
     4261 gtgaccacgc cgcctgctcc gatcaaacgc atcggcgttg cgtgcgtgat gagcgtgacg
     4321 ccatgggtct gcgcgaaatc gcgctcggcc cacgtcgcgc tcatcgattc gacgccgcgt
     4381 cgatacacca tcgtcaccga cgtcgcaccg agcttgcggc tttgtacggc ggcatccact
     4441 gccgtattgc cgccgccgat cacgacgacc cggcggccca ccggcaccgt gccgaagtca
     4501 cgcgcgctgc gcacctgttc gatgaaatcc acggcgttca tcacgcccct caggtcctcg
     4561 ccttccatcg ccaatgcgcg cacgcccgtg agaccgatcg cgaggaacac cgcatcgtgc
     4621 tgcttgcgca gcgcatcgag gtccacgtca cgcccaagcg ccacgccatg tttgatctcg
     4681 atgccgccga tcgaacacag ccattccact tcgcgctgcg caaaatcgtc gacggtttta
     4741 taggcagcga taccgtactc gttcaggcca cccgctttct cgtgggcgtc gtaaacgatg
     4801 acgtcatggc cggctagcgc gagacggtgc gcgcaggcga gtccagccgg tcccgcgccc
     4861 accaccgcga catggcggcc cgtttcagca gcgcgtttga atagcggctc accccgcgcc
     4921 atcgcccaat cggtcgcatg gcgttgcagc gcgccgatcg cgaccggctt cgcatcctga
     4981 tggttgcgaa cacaagcacc ctcgcacagg atctcggtcg gacaaacgcg cgcgcacatg
     5041 ccgcccagcg gattcgccga cagaatgtcg agcgccgcgc ccttgagatt gccgttgctg
     5101 atcttgcgaa tgaaactcgg aatatcaatc tgcgtcggac acgcgttcac gcacggcgcg
     5161 tcgtaacagt agtgacagcg gctcgccgca gcggctgcgg cacttgcgtc gagcaacggc
     5221 gcgatatcgg agaactcgca cgagagttga tcggccgata ggcgctgagg cgcaatatcg
     5281 ccggtttgct tgatggccat acacgttcct tcttcaatgt gaggacgtag ccgctcccgc
     5341 cggccgcgct ggagcggagc cgggacggca tgttttttat gggcactacg gtgcggctgg
     5401 tgaatcgacc aaagccgcga ccaataccgc aaccgctaaa gcgttatgaa accggctcgc
     5461 acgctcgttc gagcatggcg tgcaacaaga cgttggcgcc ggcttcgatc cattcgaagc
     5521 tggcgtcctc gatctcgttg tgactgatcc cgtccacgca cggcacgaat accatcgagg
     5581 tcggcgcgac ttgcgacaga tagcaggcgt catgtcccgc accggacacc atgttgcgat
     5641 gcggataacc gaagcgctcg gccgcggcgc gtacggactt cacgcaggct tcgttgaaag
     5701 cgacaggcgc gtagtagaaa atctgctcga gggcggtttc cagaccaata ccgttcgcga
     5761 tatccgccac gccttgacgc aacgcggcat ccatcttcgc gagcacggca tcatccggat
     5821 gacggaaatc gacggtgaag aacacgcggc ccggaatcac attgcgtgag ttcggataga
     5881 cctgcatcat gccgaccgtc gcgcagccga acggcgcgtt gtcgagcccg atacgattga
     5941 ccagatcgac cacgcgggcc gcgccgagca atgcatcgcg ccggcgcggc atcggtgtcg
     6001 ggcccgcatg cgcctcctgg ccggtcagcg tgatttcgta ccagcgctga ccttgcgcgt
     6061 cggtcaccac gccaatggtt ttttgctcgg cttcgagaat cggcccttgc tcgatgtgca
     6121 attcgaacgc ggcgtgcaac gggcggccac cgcaaggcag atcgcctgca tagccgatgc
     6181 gcttgagttc ctcgccgatg gtcttgccgt ccacgtcctt gcgcgagagc ccgtagtcca
     6241 gggtgaaaac accggcgaac acacctgagg cgaccattgc cggcgcgaag cgcgagcctt
     6301 cctcgttggt ccagatcacc acctcaaccg gatgttcggt ctcgatgccg tgatcgttca
     6361 gactgcggat cacttcgagt ccgccgagca cgccgtagat gccgtcaaag cggccgccgg
     6421 tcggctgcga gtccgcgtgc gaaccggtc
//
LOCUS       DATASH010001071         9660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4931045,
            whole genome shotgun sequence.
ACCESSION   DATASH010001071 DATASH010000000
VERSION     DATASH010001071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 9660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9660
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4931045"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="VHV99_28355"
     CDS             complement(<1..963)
                     /locus_tag="VHV99_28355"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX3639323.1"
                     /translation="MINPLTILVVTVLSSAMSMAVLGSLWRAVIPGVGYWISASAVAI
                     VALLAFVSQGHASPWVGIVAANELLAVSLLLVVEGCARFLSRRTRPWAAYAGALALLF
                     CIIYWTFAVPDFNARVSVVSAYHALLYAMLAVMMVRHRPANRPRYSYYFLAVAASLLC
                     VGHASRGIFYGFGLVLQSSLLQVSPSNVVFLALGILATPCLSIGVVMLAHDRLAERLE
                     RLANVDELTGALSRRAFLAAGDALLNTARRTRSPMTMAIIDIDSFKAVNDRYGHAAGD
                     QVLSHFAAFVAGNLRVGDVFGRLGGEEFGVLCPATTAAEAVSLLD"
     gene            1543..1998
                     /gene="bcsD"
                     /locus_tag="VHV99_28360"
     CDS             1543..1998
                     /gene="bcsD"
                     /locus_tag="VHV99_28360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsD"
                     /protein_id="HEX3639324.1"
                     /translation="MVPILYYLLERQISPQWRGMLTALATEFEAQIGRDELRQLMYRV
                     GCRFAAAHQLPACGSTAELAQALNLYWHDMDWGVVELSDEAESLRIVHYCAPLQAFGH
                     TALAWTPAFLEGVYQTWLSALGAQGLSVVQTSEYGTDTAIEFRLGRHSV"
     gene            2263..3297
                     /gene="bcsP"
                     /locus_tag="VHV99_28365"
     CDS             2263..3297
                     /gene="bcsP"
                     /locus_tag="VHV99_28365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsP"
                     /protein_id="HEX3639325.1"
                     /translation="MSSSSDIEKLFDHFGGDANAYQEIGRENEARAARTRWPLLVTLD
                     LTQPTIPAIGQRREPPREPLASNAQAAAVDRLDTTPKDTVSVTRARAPLFTRSHRRDI
                     PPVVAVAAAQPAAPSGASRFGALEANAEAEAQAAHSMQTAPAAARVDAAPPAATQAAP
                     AVVVPQMSPAAAQVATAPVPPLMSRASVPPVAQSRTPAMPVTQPVTSMSPNLPTPHAE
                     IPALTPRATAPTWAQAPAPVPAAAAPAWTQTLSAQAQAHAHAPTYTTASAAPKPPASI
                     LGKLFASEPELASPPQPAAPKGESAPLQSVFDRLRGTSAQTAAAPAAAAGTAPAAVSN
                     SWLVNGPRRS"
     gene            3294..4082
                     /gene="bcsQ"
                     /locus_tag="VHV99_28370"
     CDS             3294..4082
                     /gene="bcsQ"
                     /locus_tag="VHV99_28370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090540 - bacterial cellulose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HEX3639326.1"
                     /translation="MKVISVVSAKGGVGKTTLAANLASVLAASGRRVIALDLDPQNAL
                     RLHFGVPLDSIDGLSRATLTGDPWQSVMFDGIDGVTVLPYGALLEVDRRRFEAHIDQD
                     PGWLSQALQTLRLDASDIVIIDTPPGSSAYVRTALCAATFTLNVVLADAASYAAIPLM
                     ERLIETYAAPRPEFGGEGYVINQIDQSRQLTKDVLKVLRQMLGNKLFPGVIHLDEGVS
                     EALACDTTLIHYDPLSQAAADFRACGNWLTAALDAIAASPRSVA"
     gene            4079..6283
                     /gene="bcsA"
                     /locus_tag="VHV99_28375"
     CDS             4079..6283
                     /gene="bcsA"
                     /locus_tag="VHV99_28375"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HEX3639327.1"
                     /translation="MSTPSSQGLEGAEPSRLERFVDARFWNSRVVTALVTLFALTMLY
                     FVFTVPLAFYEQLTFATCCFITALWFRRLPGRYATMVMIMLSVVTSGRYMYWRLTSTT
                     YWEHPLDAAWGLLLVAAEVYSTIVLLLGYFQTAWPLKRTPMPLPASRDLWPTVDVFIP
                     TYNEPLTVVKPTIFAALALDYPADKISIHVLDDGRRPEFKAFCEEVGVNWTIRTHNRH
                     AKAGNINEALKITQGEYLAIFDCDHIPTRSFLQIGLGWFLRDKLLSMLQTPHHFFSAD
                     PFERNLGTFRKVPNEGELFYGLVQDGNDLWNATFFCGSCALLRRTIVEEIGGIAVETV
                     TEDAHTALKLHRLGYTTAYLAIPQAAGLATESLSSHIGQRIRWARGMTQIFRIDNPLT
                     GKGLKIGQRLCYLNAMMHFFYGIPRLVFLTAPLSYLFFGAHVIEAAASTIAIYAMPHM
                     MHASITNSRMQRSFRHSFWAEVYESVLASYITAPTLLALINPKLGKFNVTAKGGQIEK
                     NYFDWAISRPYLFLLLLNLIGFVVGIVHIYLNWHIRSVVNTTFLNLGWTTYNMLILGA
                     SVAAASERRQIRAVHRVAMQMPVMLKFSTGRTLACETIDYSEGGVGVALPARIEVPMH
                     ERVTVSLFRGDEEYAFPATVGFTEPGRVGLRFSTMTHEQEYEFVKTTFARADAWTGWA
                     EGRQQDTPLRGLSHVLTVGARGIAGLFEHLYADLRSSMKGRPVDVKKLKIKD"
     gene            6287..8848
                     /gene="bcsB"
                     /locus_tag="VHV99_28380"
     CDS             6287..8848
                     /gene="bcsB"
                     /locus_tag="VHV99_28380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HEX3639328.1"
                     /translation="MGNRMAKSNVERSNHERNGMAPEPRRFGRLRSQRLMRGLACWLA
                     LQTALAAPLASAAEVAAAAMSGASASQAGQAPAAQGAPAARVATGVGNVPAPSAAVPY
                     DPNTIAQAQLATPTPALAAASLKALGNRTPTTAEPGTLVPGGRRQTLTFADLGARDPL
                     QLRGTDGQNGVAFSVRGDEVVTGAILHLVYSYSPALLANLSQLKVLVNGEVAATLPVP
                     HEQAGMLVARDVSIDPRFITEFNHLNVQLIGHYTTSCEDPANSSLWATVSNASSLDLT
                     YASLASKPDLAALPQPFFDRRDVRRLELPFVFPQKPGAATLEAGGIVASWFGALAGYR
                     GAVFPAQLDNAPLSGNAVVFATDDQRPAGVTIPAISGPTIAVVDREAPARGKLLLVLG
                     RTEAELKTAARSLGIGQNTLTGPSATITQLNELAPRAPYDAPNWLPTNRPVRFGELAD
                     ARELTVSGYDADAVRVNLRVPPDLFMWHTKGAPIDLRYRYTVRPLRDRSSLNVSVNDG
                     FVQALPIPAESASVFELSHYFANVLPDKTAEARRTVYVPPLLLTPRAQVRLHFYYDIP
                     NTGECHGRLLDNVVGAIDPNSTIDLSSFPHYMALPDLAAFANSGFPFTRMADLSETAV
                     VLPNEADSSDYSLYLLTMGRMGASTGYPVNGVTVGTADDVDKYADKDLLIFGAPGKQP
                     LLQRWARSMPFSSDGDSRTFALSDVVFKLEDWWHGERGVERSPARADLTLVSSNGAAL
                     LTGFESPLQKNRSAVALVSAAGQSDADLSAALLDADVLPKIQGAMAVIHGRDVTITSN
                     GDAYYVGHLSPQEYLRWALSSHPLLLMLGGVLAALIIAGLFYRTLRSIAARRLKD"
     gene            8859..>9660
                     /gene="bcsZ"
                     /locus_tag="VHV99_28385"
     CDS             8859..>9660
                     /gene="bcsZ"
                     /locus_tag="VHV99_28385"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013341569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HEX3639329.1"
                     /translation="MRFRINRKIGIALTLGMAASASFGGIVKVAQSGGAPAACGEWSG
                     YRTFVERFVQADGRVIDYSTPTQQTTSEGQSYALFFALVANDRATFDRLLAWTRTNLA
                     GNQFDAQNLRLPAWQWGKKPDGSYGVLDPNSASDSDLWIAYDLLQAGRLWRDTSYTRL
                     GEALAAQIVRQEITTLPGVGPMLLPGPQGFKTAGVTRLNPSYLPLPVLRGLAHEMPDG
                     PWSKLADSAYKLIKTTAPQGFAPDWAAWQNGQFVVDPKNGDVGSYDAIR"
BASE COUNT         1759 a         3240 c         3014 g         1647 t
ORIGIN      
        1 gtccagcaag ctcaccgctt cggcggcggt ggttgccggg catagcacgc cgaactcctc
       61 gccgcccagc cgcccgaaca cgtcgccaac ccgcaggttg ccggcgacga acgccgcaaa
      121 atggctcagc acctggtcgc cggcggcatg gccgtagcgg tcgttgacgg ctttgaacga
      181 atcgatgtcg atgatcgcca tcgtcatcgg cgagcgagtg cgccgcgccg tgttcagcag
      241 cgcgtcgccg gccgcgagaa acgcccggcg cgacagcgcc cccgtcaatt cgtcgacatt
      301 ggcgagccgc tcgagtcgct cggcgagtcg atcgtgcgcc agcatcacca caccgatcga
      361 aaggcacggc gtcgcgagaa tgccgagtgc gagaaaaacg acgttgctcg gcgacacctg
      421 cagcaagctg gactgcaaca ccaggccaaa accgtaaaag atgccgcgcg aagcatgccc
      481 gacgcataac agcgacgccg cgaccgcgag aaagtaatag ctgtagcgcg gccggtttgc
      541 ggggcggtgc cgcaccatca ttacggcaag catcgcatag aggagcgcgt gatatgccga
      601 cactacgctc acgcgtgcgt tgaagtccgg caccgcaaag gtccagtaaa tgatgcagaa
      661 cagcaacgca agcgcgccgg cgtaggccgc ccaaggccgt gtgcggcggc tcagaaaccg
      721 ggcgcagcct tcgaccacca gcagcagcga gacggccagc agttcattgg ccgctacgat
      781 acccacccag ggcgatgcat ggccttgcga gacaaaggca agcagcgcca cgatcgcaac
      841 cgcactggcc gagatccagt agccgacgcc ggggattacc gcgcgccata acgagccgag
      901 cacggccatg ctcatggcac tggataaaac cgtgacaacc agaatagtga gtggattgat
      961 cattgttatt tgagtcgaac ggcatcccca tggcgggccg tgttccagct gcctgcgctg
     1021 ttcgcgccat tgcatgaaca gcccgcggcg caagttcgtc agcttactgg cactgacgat
     1081 agttgaagtc gcaacgattc tgcaagaatt accggcaaac gcaaatatct attgcattcg
     1141 cgcaaactca aagcgttgca tttgccacga aataaaacac taaagcaacg ataaaaccgg
     1201 cgccgatagg cccatgagga cggccgctaa aacgaacacg aatactttcg caccggccag
     1261 gcacagttgg gaaggactca agccgattca cgcaggaaaa attacgctgt cgatctggat
     1321 ccaccgtcaa tgtctgcacg tataaaccag acgaatgtaa agttggctta aagtcactgt
     1381 cttaagtaga ctcctccgaa gtgtggcgga cagactagac agttcggtaa aaatacccta
     1441 gggcgcgtgc aaggcgcggc ctcccgtaac gcgtccgttt ggattcgacc gtcggcatcc
     1501 gtttttatac acggcccagg caagagccat tttccggaaa acatggtccc aattctctat
     1561 tacctgctag agcgccagat ctcgccgcag tggcgcggca tgctgactgc gctcgcgaca
     1621 gagttcgaag cgcagatcgg tcgcgacgaa cttcgccaac tgatgtatcg cgtcggctgc
     1681 cgcttcgcgg cagcgcatca attgcccgca tgcgggtcaa cggccgagct cgctcaggca
     1741 ttaaacctgt actggcatga catggattgg ggcgtcgtcg agttgtccga cgaagccgag
     1801 tctttgcgca tcgttcatta ctgtgcgccg ctgcaggcat tcggccacac ggcgctcgca
     1861 tggacgccgg cgtttctcga aggtgtctat cagacgtggt tgagcgcatt gggcgcccag
     1921 ggcttgtcgg tggtgcaaac gagcgagtac ggcacggaca ccgcgatcga gttccggctc
     1981 gggcggcatt ccgtttgagc ggaccggttt gcgaagcggt aagcgaaacg gtttgcgaag
     2041 ccggtaaatg aagccggtca gcggaccggg taagcggttc gaaagatgaa ccatcaggca
     2101 tgcgccggca agaccggctc gcgtccggca gagagtcgcc agaaacaggc agtagaggaa
     2161 cgcgccagac cggtgcgcgt gaagtggcaa tacccgcatg acagcggcgc gagtaagcaa
     2221 agggatgggg gcaacacaag tcatgaacaa gacggcggca ctatgagttc atcgagcgac
     2281 atcgagaaac tattcgatca ttttggcggc gacgccaacg cgtaccagga aatcggccgc
     2341 gaaaacgaag cgcgtgcggc gcgcacccgc tggccgctgc tggtgacgct ggacctgaca
     2401 cagccgacga tccccgcgat cggacaacgt cgtgagccgc cccgcgagcc attggctagc
     2461 aacgcgcagg cggcggccgt ggatcgtctg gacacgacgc ctaaagacac ggtctcggtc
     2521 acgcgcgcga gggcgccgct gttcacccgc tcgcatcggc gggacattcc gccggtcgtt
     2581 gccgtcgccg ctgctcagcc tgccgcgccg agcggtgctt cgcgtttcgg cgcgctcgaa
     2641 gcgaacgccg aggccgaggc gcaagccgca cacagcatgc aaacagcgcc ggccgcggcg
     2701 cgtgtcgacg cggcgccccc ggcggcaacc caggctgcgc ccgccgtcgt tgtgccacag
     2761 atgtcgccgg cggccgcgca agtcgccact gcaccggttc ccccgctcat gtcgcgtgcg
     2821 agtgttccgc cggtggcgca gtcccgtacg ccggccatgc ctgtcacgca acctgtgacc
     2881 agcatgtcgc caaacctacc gacgccgcac gccgagatcc ccgccctcac gccgcgcgcc
     2941 accgcgccga catgggcgca agcgcctgct cccgttcctg ccgctgccgc tccagcatgg
     3001 acgcaaacgt tatcggctca ggctcaggct cacgctcacg cccccaccta caccacggcg
     3061 tccgcggcgc cgaagccacc cgcttcgatc ctcggcaaac tgttcgcttc ggagcccgaa
     3121 ctggcgtcgc cgccgcaacc cgccgcgccc aaaggcgaat ccgcaccgct gcaatcggtc
     3181 ttcgaccgtc tgcgtggcac gtccgcccaa acggccgctg ctccggccgc tgctgctggc
     3241 accgccccgg ccgccgtctc caattcgtgg ctggtcaacg gccctcgtcg ctcatgaaag
     3301 tcatctcggt cgtgtccgcc aaaggcgggg tcggtaaaac caccctcgcc gccaatctcg
     3361 cttccgtgct ggccgccagc ggccgtcgcg tgattgccct cgacctcgat ccgcagaacg
     3421 cgctgcgtct gcatttcggc gtgccgctcg acagcatcga cggtttgtcg cgcgcgacgt
     3481 tgacgggcga cccatggcaa tcggtgatgt tcgacggcat cgacggtgtg accgtgctgc
     3541 cctatggcgc gctgctcgaa gttgatcgcc gccgcttcga agcgcacatc gaccaggacc
     3601 cggggtggct ctcgcaagcg ctgcaaacgc tgcggctcga cgcgtccgac atcgtcatca
     3661 tcgacactcc gccgggttcg tcggcatacg tgcgcactgc gttgtgcgcg gccaccttca
     3721 cgctgaacgt ggtgctcgcc gacgccgctt cctacgcggc cattccgttg atggaacgcc
     3781 tgattgaaac gtatgcggcg ccacgccccg agttcggtgg cgaaggttac gtgatcaacc
     3841 agatcgacca gtcgcgccag ttgaccaagg acgtcctcaa ggtcttgcgc cagatgctcg
     3901 gcaacaagct gttcccgggc gtgatccatc tggacgaagg cgtgagcgaa gcgctcgcgt
     3961 gcgacaccac gctgattcac tacgatccac tcagccaggc ggccgccgat tttcgcgcgt
     4021 gcggcaactg gctgacggcg gcgctcgatg cgatcgccgc ctcgccgagg agcgtcgcat
     4081 gagcacgcct tcttcgcagg gcctcgaagg cgccgagcca tcgcggctcg aacgcttcgt
     4141 cgacgcgcgc ttctggaaca gccgggttgt gacggcgctg gtcacgctgt tcgcgctgac
     4201 gatgctgtac ttcgtgttca cggtgccgct cgcgttctac gagcaactga cgttcgcgac
     4261 ctgctgcttt atcaccgcgc tgtggttccg ccgcctgcct ggccgttacg cgaccatggt
     4321 gatgatcatg ctctcggtgg tcacgtcggg ccgctatatg tactggcggc tgacttcgac
     4381 aacgtactgg gaacatccgc tcgacgccgc ctggggcctg ctgctggtcg cggccgaagt
     4441 ctattcgacc atcgtgctgc tgctcggcta cttccagacc gcatggccgc tcaagcgcac
     4501 gccgatgccg ctgcccgctt cgcgcgacct gtggccgacc gtcgacgtgt tcatcccgac
     4561 ctacaacgag ccgctcacgg tggtgaagcc gaccatcttt gccgcgctcg cgctcgacta
     4621 tccggccgac aagatttcga tccacgtgct cgacgacggg cgccgccccg agttcaaggc
     4681 gttctgcgag gaagtcggcg tcaactggac gatccgcacg cacaaccgcc acgcgaaggc
     4741 cggcaacatc aatgaagcct tgaagatcac gcaaggcgag tacctcgcga tcttcgactg
     4801 cgatcacatt ccaacccgct cgttcctgca gatcggcctc ggctggttcc tgcgcgacaa
     4861 gctgctgtcg atgctgcaaa cgccgcacca cttcttctcc gccgatccgt tcgaacgcaa
     4921 tctcggcacc ttccgcaagg tgccgaatga aggcgaactg ttctacggcc tcgtgcagga
     4981 cggcaacgat ctgtggaacg ccacgttttt ctgcggctcg tgcgcgctgt tgcgccggac
     5041 cattgtcgaa gagatcggcg gcatcgcggt ggaaacggtg actgaggacg cgcacaccgc
     5101 gttgaagctg caccgcctcg gctacaccac ggcgtatctg gccattccgc aggcggcggg
     5161 tctcgccacc gagagtctct ccagccacat cggtcagcgg attcgctggg cgcgcggcat
     5221 gacgcagatt ttccggatcg acaatccgct caccggcaag ggcctcaaga tcggccagcg
     5281 gctctgttat ctgaacgcga tgatgcactt cttctacggc atcccgcgtc tggtgttcct
     5341 caccgcgccg ctgtcgtatc tgttcttcgg cgcgcacgtg atcgaagccg ccgccagcac
     5401 gatcgcgatc tacgcgatgc cgcacatgat gcatgcgagc atcaccaatt cgcggatgca
     5461 gcgctcgttt cgccattcgt tctgggccga ggtgtatgaa tcggtgctgg cgtcgtacat
     5521 caccgcgcct accttgctcg cgctgatcaa cccgaaactc ggcaagttca acgtgacggc
     5581 caagggcggc cagatcgaga agaactactt cgactgggcg atctcgcggc cgtatctgtt
     5641 cctgctgttg ctgaacctga tcggcttcgt cgtggggatc gtgcatatct atctgaactg
     5701 gcacatccgc agcgtggtga acacgacctt cctgaacctc ggctggacca cctacaacat
     5761 gctgattctc ggcgcgagcg tagccgcggc aagcgagcgc cggcagattc gcgcggtgca
     5821 ccgcgtggcg atgcaaatgc ccgtgatgct gaagttctcg accggccgca cgctcgcctg
     5881 cgaaaccatc gactactcgg aaggcggcgt cggcgtggcg ctgccggcca ggatcgaagt
     5941 gcccatgcac gagcgcgtga ccgtctcgct gtttcgcggc gacgaggaat atgccttccc
     6001 cgccaccgtc ggcttcacgg agccgggccg cgtgggcctg cgcttctcga cgatgacgca
     6061 cgaacaggaa tacgagttcg tcaagaccac cttcgcgcgc gccgacgcgt ggaccggctg
     6121 ggccgaagga cgtcagcagg acacgccgtt gcgcggtctg tcgcacgtgc tgacggtcgg
     6181 cgcgcgcggc attgcgggtc tcttcgaaca tctttatgcc gaccttcgaa gctcgatgaa
     6241 aggccgtccg gtggacgtca agaagctaaa aatcaaagac tgatctatgg gcaacaggat
     6301 ggcgaagtca aacgtcgaac gctcgaatca cgagcgcaat gggatggccc cggagccgcg
     6361 tcgttttggc cgtctccgtt cgcaacggtt gatgcgcggg ctcgcctgct ggctcgcatt
     6421 gcagaccgcg ttggcggcgc cgctcgcgtc ggcggccgaa gtggccgcgg cagccatgag
     6481 cggtgcgagc gcgtcgcaag ccggccaggc gccggcggcc cagggcgcac cggctgcgcg
     6541 cgtcgccacc ggcgtgggca atgtgcccgc gccgagcgcg gccgtcccct acgatccgaa
     6601 cacgatcgcg caagcgcagc tggccacgcc gacaccggcg ctcgccgcgg cgtcgctgaa
     6661 agccttgggg aacagaacgc cgaccacggc cgaaccgggc acgctggtgc cgggtggccg
     6721 ccgtcaaacg ctcactttcg ccgacctcgg cgcacgcgat ccgctgcaac tgcgcggcac
     6781 cgacggtcag aacggcgtcg cgttttccgt gcgcggcgac gaagtggtga ccggcgcgat
     6841 cctgcatctg gtctatagct actcgcccgc gctgctcgcg aatctttccc agctgaaagt
     6901 gctggtgaac ggcgaagtcg ccgcgacctt gccggtgccg cacgaacagg ccggcatgct
     6961 ggtggcgcgc gacgtctcga tcgatccgcg tttcatcacc gaattcaacc acctgaacgt
     7021 gcagttgatc ggccattaca cgacgagttg tgaagacccg gcgaattcgt cgttgtgggc
     7081 caccgtcagc aatgcgagtt cgctcgatct gacctatgcg tcgctggcca gcaagccgga
     7141 tctcgccgcg ctgccgcaac cgttcttcga ccgccgcgac gtgcgccgtc tcgagttgcc
     7201 cttcgtgttc ccgcaaaaac cgggtgccgc cacgctcgaa gcgggcggca tcgtcgcctc
     7261 gtggttcggc gcgctcgccg gctatcgcgg cgcggtgttc ccggcgcaac tggataacgc
     7321 gccgctatcg ggcaacgcag tggtcttcgc gaccgacgac cagcgtccgg ccggcgtaac
     7381 gattccagcg atctccggac cgaccatcgc ggtagtggat cgtgaagcgc cggcgcgcgg
     7441 caagctgctg ctcgtgctcg gccgcaccga agccgaactg aaaaccgccg ccaggtcgct
     7501 cggcatcggc cagaacacgc tgactggccc gagcgcgacg atcacacagt tgaacgaact
     7561 cgcgccgcgt gcgccgtacg acgcgccgaa ctggctgccg actaaccgtc cggtgcgttt
     7621 cggcgaactc gccgacgcgc gcgagctgac cgtatccggc tacgacgccg acgccgtgcg
     7681 cgtcaatctg cgcgtgccgc ccgacctgtt catgtggcac accaagggcg cgccgatcga
     7741 tctgcgctac cgctacacgg tgcgtccgct gcgggatcgt tcgtcgctga acgtgagcgt
     7801 caatgacggc ttcgtgcagg cgctgccgat tccggccgag tcggcctcgg tgttcgagct
     7861 gagccattac ttcgcgaacg tcttgccgga caagaccgcc gaagcgcgcc gcacggtcta
     7921 tgttccgccg ctgctgctga cgccgcgcgc ccaggtgcgc ctgcatttct actacgacat
     7981 tccgaacacc ggcgaatgtc acggccgtct gctggacaac gtggtcggcg cgatcgatcc
     8041 gaactcgacc atcgacctct cgtcgttccc gcattacatg gcgctgcccg atctcgcggc
     8101 gttcgccaac agcggcttcc cgttcacgcg gatggccgac ctgtccgaga ccgcggtggt
     8161 gctgccgaac gaggccgatt cgagcgacta cagcctgtac ctgttgacca tgggccgcat
     8221 gggcgcatcg acgggatacc cggtgaacgg cgtcacggtc ggcaccgccg acgacgtcga
     8281 caagtacgcc gacaaggacc tgctgatttt cggcgcgccc ggcaagcaac cgctgttgca
     8341 gcgctgggcc agatcgatgc cgttctccag cgacggcgat tcgcgcacct tcgcgctctc
     8401 ggacgtcgtc ttcaagctcg aggactggtg gcatggcgaa cgcggtgtcg aacgctcgcc
     8461 ggcgcgcgcc gacctgacgc tcgtgagttc gaacggcgcc gccctgctga ccggtttcga
     8521 atcgccgctg caaaagaacc gcagcgctgt cgcgctggtg agcgcggccg gccagtcgga
     8581 cgccgacctc tcggccgcgc tgctcgacgc cgacgtgttg ccgaagattc aaggcgcgat
     8641 ggccgtgatc catggccgcg acgtcaccat cacgtcgaac ggcgatgcgt actatgtcgg
     8701 ccatctgtcg ccgcaggagt atctgcgctg ggcgctgtcg tcgcatccgc tgctgttgat
     8761 gctgggcggc gtgctcgccg cgctgatcat cgcgggcctc ttctaccgca cgctgcgctc
     8821 gatcgccgcg cgccgcctga aggactgata ggacgggtat gcggtttcga atcaacagga
     8881 aaatcggcat cgcgctgacg ctcggaatgg cggcgtcggc gagtttcggc ggcatcgtga
     8941 aagtcgcgca gagcggcggc gcgccggccg catgcggcga atggagcggc taccgcacat
     9001 tcgtcgaacg cttcgtgcag gccgacggcc gcgtgatcga ctactcgacg ccgacccagc
     9061 agaccacttc ggaaggccag tcgtacgcgc tcttcttcgc gctggtcgcg aacgaccgcg
     9121 cgaccttcga ccggctgctc gcgtggaccc gcaccaatct cgcgggcaat cagttcgacg
     9181 cgcaaaacct gcggctgcca gcgtggcagt ggggcaagaa accggacggc tcgtacggcg
     9241 tgctcgatcc gaactccgca tccgactccg acctgtggat cgcctacgat ctgctgcaag
     9301 ccggccgcct gtggcgcgat acgagttaca cgcgactcgg cgaggcgctg gccgcgcaga
     9361 tcgtgcgcca ggaaatcact acgttgccgg gcgtcggccc gatgctgctg ccaggaccgc
     9421 aaggcttcaa gaccgccggc gtgacacgcc tgaatccgag ttatctgccg ttgccggtgc
     9481 tgcgcggact cgcgcacgag atgccggacg gtccgtggag caagctcgcg gacagcgctt
     9541 acaagctcat caagaccacc gcgccgcaag gcttcgcgcc cgactgggcg gcatggcaaa
     9601 acggccagtt cgtggtcgat ccgaaaaacg gcgacgtcgg cagctacgat gcgatccgcg
//
LOCUS       DATASH010001072         9429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5726162,
            whole genome shotgun sequence.
ACCESSION   DATASH010001072 DATASH010000000
VERSION     DATASH010001072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 9429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9429
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5726162"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VHV99_28390"
     CDS             <1..241
                     /locus_tag="VHV99_28390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HEX3639330.1"
                     /translation="AALQESGDKVHLVLIGHECTAETRTQLSERAIDVILDQDVRGIV
                     HWAGLAAINFLNDVRGALAIPTPETRIYLRENAHA"
     gene            355..1686
                     /locus_tag="VHV99_28395"
     CDS             355..1686
                     /locus_tag="VHV99_28395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008348942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX3639331.1"
                     /translation="MLRLTSARVVAASLISLGLGTATGAQAQDKQLTLCWAAWDPANA
                     LVELSKDFTTKTGIKMKFEFVPWTSYADRFINELNSHGKLCDLIIGDSQWIGGAAVNG
                     HYVKLNDFFTRNKISMDDFVPATVVGYSEWPKNTPNYWALPAMADAVGWTYRKDWFAR
                     PELRAEFKQKYNRELAPPTTLDELKQTAEFFQGRKIDGKTVYGVYIFTERGSEGITMG
                     VTNALYNFGFEYQDPKKPYHLDGFVNSPGAVKGLEYYKALYKCCTAPGMTNAYMQEGL
                     DAFKSGQVAMQMNWFAFFPGLYKDPNVGGDKIGFFVNPRELKQYTQLGGQGISVVSYS
                     DHKEDALEYIKWFAQPDVQKKWWQLGGYSAAKSVVDAPDFPKSAPFAPAFLKSMAIVK
                     DFWAEPAYAELLLDMQKRVHDYVVADKGTAKDALDLLVKDWTKVFKQEGKY"
     gene            1758..2714
                     /locus_tag="VHV99_28400"
     CDS             1758..2714
                     /locus_tag="VHV99_28400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007731678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX3639332.1"
                     /translation="MLADKPFSPTSLDVGASTRIAKRLSGLSDRAIAWLFILPTILLL
                     LAINIFPLIWAIRLSFTNFKSNMPSVPARFVGIDNYVDILTDEDIWYAMQVTARFVFW
                     SVGLEVLLGFGLALLINRQFRGHSFWTTLILLPMMLSPAVVGNFWTFLLQPQTGLFND
                     IVSFFTGIAPGSFQMIGDVALAPWTIVMVDTWMWTPYVMLICLAGLRSIPDYIYEAAE
                     VDRATPWRQFWSITLPMTMPFLMLAVLFRGIENFKMFDMVNLLTSGGPGSVTETVSIT
                     LKRAAFEKWQTGYSSALAIILFVTVFGAANIYVKALNRIKQR"
     gene            2711..3664
                     /locus_tag="VHV99_28405"
     CDS             2711..3664
                     /locus_tag="VHV99_28405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX3639333.1"
                     /translation="MRPTTTQHSVVASSPRAKRFAAAVVITYALIATLPMVWIFLTSF
                     KTQEDAIAYPPVVLFQPSMEGYVNLFTIRSRQTPEFIASLPPAQTWYEREVRKRNMVI
                     AGPSKVLPRFMNSLVIGFGSTFLAVFLGTLTAYAFSRFKVPLADDLLFFILSTRMMPP
                     IAVAIPIYLMYRALGLSDSYVGMIVLYTAVNVSLAVWLLKGFMDEIPREYEEAALVDG
                     YTRLQAFVKVVLPQAMTGIAATAIFCLIFAWNEYAFALLLTSGDAQTMPPFIPFIIGE
                     GGQDWPAVAAATTLFVLPILFFTVLLRKHLLRGITFGAVRK"
     gene            3661..3855
                     /locus_tag="VHV99_28410"
     CDS             3661..3855
                     /locus_tag="VHV99_28410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639334.1"
                     /translation="MKREVIPPHRWLLRRSYREGASTVLIGLGMAMLVQPFSLALFTW
                     SFPVILVGALAFVVTSHFPD"
     gene            3859..4962
                     /locus_tag="VHV99_28415"
     CDS             3859..4962
                     /locus_tag="VHV99_28415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3639335.1"
                     /translation="MSTIVLAHLHKRFDDFVAVRDTSLIIGAGRFVVLLGPSGCGKTT
                     TLRMIAGLELPTSGQILIDGEDVTALRARQRDIAFVFQMFALYPHMTVRNNIAFPLKN
                     EHVSRTEIAARVNAAAHMLRIENILDRKTGGLSGGDRQRVALGRAIVRQPKAFLMDEP
                     LGTLDADFRELMCLELRKLHNALAATTVYVTHDQSEAMAMADDIVVMNQGEVLQAASP
                     HEIYHFPATVFVGNFIGSPPMNFLPVDGAVMAGQEQVSLHGAAVSVPRCDAAAARVLL
                     GIRPEHIVIDERGPLRGQVIADEYLGSHQVLAVETVLGVVRVRASKDDGVAAGTTLGL
                     SFRKERTLLYDAATERLLPGAAATLSASGEGHG"
     gene            4955..6001
                     /locus_tag="VHV99_28420"
     CDS             4955..6001
                     /locus_tag="VHV99_28420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3639336.1"
                     /translation="MAEIQLRNVSKRFGNIVAVDDLSIDVKDGEFVVLLGPSGAGKTT
                     TLRLIAGLERPDAGDVLIDGAVATGVHPSDRDVAFIFQQYSLYPHLTVFGNLAFPLRS
                     PRRRSSEEQVRARVHAVAQLLHMEAKLDNMATHLSGGEMQRVAIGRALVREPKVFLMD
                     EPLSSLDAKLREELRIELKRLHRTIGATILYVTHDQVEATTLADRIGILEHGRLVQLG
                     TPREVYGNPVSLSAAQRLGSPPINLLPPTLFDSAAVPTGTATVAIRPEDVVLHDGDAR
                     DAPGTHHARDALDLKVLEYSPLRHLLILDRAGTAVVATTMAERNFSPGQLVGVSLPAR
                     SLLYFRADGRRIPT"
     gene            5998..6636
                     /gene="dhaL"
                     /locus_tag="VHV99_28425"
     CDS             5998..6636
                     /gene="dhaL"
                     /locus_tag="VHV99_28425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009769626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HEX3639337.1"
                     /translation="MNGKTVMECIDAAYAALKEHTDEIAVLDQQIGDGDHIFNLLRGM
                     EALLAVRADIEAETLGAALELAANKVLSTVGGSSGPLFFSLLNGMAKASANNPLDVAG
                     TALMFVAGVEAVGQRGKTGVGSKTMMDVLIPVAARFKELATIDSPTLRKTVLDELPQV
                     AERNMLATRDMLATKGRASFLGERSRGHIDPGARSSQIMITAVCAYLARNRA"
     gene            6651..7640
                     /gene="dhaK"
                     /locus_tag="VHV99_28430"
     CDS             6651..7640
                     /gene="dhaK"
                     /locus_tag="VHV99_28430"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018435071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEX3639338.1"
                     /translation="MKKFINHVDDFLAESLAGFASAHSDLVVLNNEPVYVRRKTLKPG
                     KVALISGGGSGHEPLHSGFVGYGMLDAACPGQVFTSPTPDQMMAAADAVDTSAGVLFI
                     VKNYSGDLMNFEMASEMSEIPSAMVLINDDVAVENSSYTTGRRGVAGVVIVEKLVGSM
                     AESGADLEQCKAFGDRINKRTASMGVAFSSCTVPAAGTLTFKIGDDEIEVGVGIHGEP
                     GRRRAKFSGADAIAGELLKAIVDDLKPQAGAELLVLINGLGGTPLGELYLLFNSTRAW
                     LQQRDLKVGRAQVGALTTSLEMAGASITLCVLDDEMARHWDSPVHTPSLRWGM"
     gene            7971..9221
                     /locus_tag="VHV99_28435"
     CDS             7971..9221
                     /locus_tag="VHV99_28435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3639339.1"
                     /translation="MTTFLLRNGALLDPTRPDLLEGFEVLVEDGFVREVSDKPITSRN
                     AHVIDVKGKTIMPGLIDLHVHVVAIEFNLPRVATLPNVLVTLRAVPIMRAMLRRGFTT
                     VRDAGGAGYPFKQALESGLIEGPRLFVSGRALSQTGGHADIRARSDYMPSDSPCGCCV
                     RVGALGRVADGVDEVRRAVREELQMGADQIKIMASGGVASPTDPVGAFGYSEDEIRAI
                     VAEAAGRGTYVLAHAYTPAAIARAVRCGVRTIEHGNLIDEETARLVAEHGAYVVPTLV
                     TYDALASEGEKYGLPPASIAKIADVHGAGMHSIEIMKRAGVKMGFGTDLLGEAQRLQS
                     DEFRLLAEVLSPAEVIASATIVGAEVLGMQDKLGRIMPGAHADLLVVDGNPLKSVDCL
                     LGQGEHIPLVMKDGRLFVNELESS"
     gene            9281..>9429
                     /locus_tag="VHV99_28440"
     CDS             9281..>9429
                     /locus_tag="VHV99_28440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3639340.1"
                     /translation="MSMIAARIERLPFSGFHRRLLLMGGLGYTFDAMDAAVLAFLLPV
                     LRTQWG"
BASE COUNT         1791 a         2720 c         2951 g         1967 t
ORIGIN      
        1 agcggcattg caggagtccg gcgacaaggt tcacctcgtc ctgattggcc acgaatgcac
       61 tgcggagacc cgcacccaac tgtcggaacg tgcgatcgac gtgatactcg atcaggacgt
      121 gcgcggcatt gttcattggg ctgggctcgc cgcgattaat tttcttaacg acgtccgggg
      181 ggcgcttgcc atccccacgc cggaaacccg gatttatctt cgtgagaatg cgcatgcctg
      241 acgctcgcga cacgcggacc gacttctgat ctgccagagc cgaaatacgg tgcccggcgc
      301 atgggcgctg aatgtcatca ggttggagac ccgagaaaga caaggagaag agacatgtta
      361 cgtctaacgt cagcgagggt agtggcggca agcctcatat cgctggggct cggcacggca
      421 acgggcgcgc aagcacagga caagcagttg acactctgct gggcggcatg ggatcccgcc
      481 aatgcactgg tcgaactgtc gaaggatttc acgaccaaga ccggcatcaa gatgaagttc
      541 gagttcgtgc cgtggaccag ttatgccgac cgcttcatca atgaactcaa ttcgcacggg
      601 aagctgtgtg atctgatcat tggcgacagt cagtggatcg gcggcgcggc ggtcaatgga
      661 cattacgtca agctcaacga cttcttcacc aggaacaaga tctccatgga cgacttcgtg
      721 ccggcaacgg tggtgggata ttcggagtgg ccaaagaata cgccgaacta ttgggcctta
      781 cctgcgatgg ctgacgcggt cggatggact taccgtaagg actggtttgc gcgtccagag
      841 cttcgcgcag aattcaagca gaaatacaac cgtgagctcg cgccgcctac cacgctcgat
      901 gaactcaagc agactgcgga attcttccag ggtcgaaaga ttgatggcaa gacggtctat
      961 ggcgtttata tcttcacgga gcgtggatcc gaggggatca cgatgggcgt gaccaacgcc
     1021 ttgtacaact tcggctttga gtatcaggat cccaagaagc cctatcacct ggatggcttc
     1081 gtgaactcgc caggcgcggt gaaggggctc gaatactaca aggcacttta taagtgctgc
     1141 acggcgccag gcatgaccaa cgcctacatg caggaagggc tcgacgcgtt caagtcgggt
     1201 caggtggcga tgcagatgaa ctggtttgcg ttctttccag ggctttataa agatccgaat
     1261 gtcggtggcg acaagatcgg cttcttcgtc aatccgcgtg agctgaagca atacacgcag
     1321 ttgggcgggc agggcatctc agtcgtctcg tactcggacc ataaagaaga cgcgctcgaa
     1381 tatatcaagt ggtttgcgca acctgacgtg cagaagaaat ggtggcaact gggcggctat
     1441 tctgcggcca aatcagtggt cgacgcaccg gactttccga agagcgcgcc gttcgcaccc
     1501 gcattcctga agtcgatggc aatcgtcaaa gacttctggg cagagcccgc ttatgccgaa
     1561 ctgttactcg acatgcagaa gcgtgtgcac gattacgtcg tggccgataa gggcacggcc
     1621 aaagacgcgt tggatctgct ggtgaaagac tggaccaagg tctttaagca agaagggaag
     1681 tattagcttt tagctggact tgactggacc ggcgacggct gaatggcgcg tctctggtcc
     1741 gcttgaagga gccgggcatg ttggcagata agcctttttc tcccacgagt ctcgacgtgg
     1801 gagcgtcaac gcgcatcgcg aagcggctaa gcggactgtc ggatcgggcc attgcgtggc
     1861 tcttcattct tccgaccatt ctgctgcttc tcgcgatcaa catcttcccg ttgatctggg
     1921 cgattcgact gtcgttcacc aacttcaagt cgaacatgcc gagcgtgccg gcgcgttttg
     1981 tcggtatcga caattatgtc gacatcctga ccgacgaaga tatctggtac gcgatgcagg
     2041 tgaccgcgcg tttcgtgttc tggtcggtcg ggttggaggt gttgctgggt ttcggccttg
     2101 cattgctgat caaccggcag ttccgcggac atagtttctg gaccacgcta atcctgctgc
     2161 cgatgatgtt gtcgcctgct gtagtgggca acttctggac gttcctgctg caaccgcaga
     2221 cggggctttt caacgatatc gtcagcttct tcaccggcat cgcgccgggg tcgttccaga
     2281 tgatcggcga cgtagcgctc gcgccgtgga ccattgtgat ggtcgatacg tggatgtgga
     2341 caccgtacgt catgctcatc tgcctggcgg gcctgcgttc gattcccgac tatatctacg
     2401 aagcagcgga agtggaccgc gcgacgccgt ggcgtcaatt ctggtcgatc acgttgccca
     2461 tgaccatgcc gttcctgatg ctggcggtat tgtttcgcgg catcgaaaac ttcaagatgt
     2521 tcgatatggt gaacctgctg acatcgggcg gcccgggctc ggttaccgaa acggtatcga
     2581 ttacgctcaa gcgtgccgcg ttcgaaaagt ggcagacagg ttattcgtcc gcgctcgcaa
     2641 tcattctgtt cgtgacggta ttcggtgcgg cgaatattta cgtgaaagca cttaaccgga
     2701 tcaaacagcg atgagaccga ctacgacaca acattccgtg gtcgcgtcat ctccgcgcgc
     2761 caagcgcttc gcggctgcgg tcgttatcac gtatgcgctg attgctacgt taccgatggt
     2821 ctggatcttc ctgacgagct tcaagacgca ggaagatgcg atagcgtatc cgcccgtcgt
     2881 gctgtttcag ccttccatgg aaggctatgt caacctgttc acgatccgct cgcggcagac
     2941 acctgagttc attgcgagcc tgccgcctgc gcagacctgg tatgagcgtg aggtacgcaa
     3001 gcgcaacatg gtgatcgccg gaccttcgaa ggtattgccg cgcttcatga attcgcttgt
     3061 cattgggttc ggttcgacgt ttcttgcggt atttctcggc acgctgacgg cctacgcgtt
     3121 ttcgcgcttc aaggtaccgc tggccgacga tctgctgttc ttcattctct cgacccggat
     3181 gatgccgccc attgcagtcg ccattccgat ctacctgatg tatcgcgcgc tgggcttgag
     3241 cgacagctat gtcggcatga ttgtgctgta cacggcggtg aatgtctcat tagcggtgtg
     3301 gctgctcaaa ggtttcatgg atgagatccc gcgcgagtac gaagaggccg cgctagtgga
     3361 tggctacacg cgtttgcagg ccttcgtgaa ggtggtattg ccgcaagcga tgacgggcat
     3421 tgccgcgaca gcgatcttct gcctgatctt tgcatggaac gaatacgcat ttgcattgct
     3481 gctgacaagc ggcgatgcgc agaccatgcc gccattcatt ccattcatca tcggagaagg
     3541 cggtcaggac tggccggccg ttgcagctgc aacaacatta ttcgtgctgc cgattctctt
     3601 tttcaccgtt ctgctgcgca agcatctgtt gcgcggcatc accttcggag ccgtgcgcaa
     3661 atgaaacgcg aagtcattcc gccgcaccgc tggctgttac gtcgcagcta ccgcgagggt
     3721 gcgtcgacgg tactgatcgg cttaggtatg gcgatgcttg tgcagccgtt ttcgttggcg
     3781 ctcttcacct ggtcctttcc ggtcattctg gtgggtgcgc tcgcgttcgt cgtcaccagt
     3841 cattttcctg actgaatcat gtccacgatc gttctcgctc atctccacaa gcgcttcgac
     3901 gattttgtcg cggtgcgcga taccagcctg atcattggag cgggacgctt cgtcgtgctg
     3961 ctcggaccgt cggggtgcgg caagaccaca acgctgcgga tgattgcggg gctggaactg
     4021 ccgacttccg ggcagatcct gatcgatggt gaggacgtca ctgcgttgcg tgcgcgccaa
     4081 cgcgatattg ccttcgtgtt ccagatgttt gcgctgtatc cacatatgac ggttcgcaat
     4141 aacatcgcgt ttccgctgaa gaacgaacac gtgtcgcgca ccgagattgc cgcccgcgtc
     4201 aacgcagcgg cgcatatgct gcgcatcgag aacattctcg atcgtaagac cggcggcctg
     4261 tccggtggcg accggcaacg tgtggcgctc ggccgcgcga tcgtgcggca accgaaagca
     4321 ttcctgatgg acgagccgct cggcacgctt gatgcggact ttcgtgaact gatgtgcctg
     4381 gagttgcgca aactgcacaa cgcgctcgcc gccaccacgg tgtacgtcac acacgatcag
     4441 agtgaggcga tggccatggc ggacgatatt gtggtgatga atcagggtga agtgttgcag
     4501 gcggcctcgc cccatgagat ctatcacttc cccgcgactg tgttcgtcgg caacttcatc
     4561 ggcagtccgc ccatgaattt cttgcccgtt gacggagcgg ttatggcggg ccaggagcag
     4621 gtgagtctgc atggcgcggc ggtgtccgtg ccacgttgcg atgccgccgc cgcccgcgtg
     4681 ttgttgggaa tcagaccgga gcacatcgtg atcgacgagc gcggcccgct gcgcggtcag
     4741 gtgatcgccg acgaatatct cggctcgcac caggtgttgg cggttgaaac cgtgctcggc
     4801 gtcgtgcgcg tgcgggcaag caaagacgat ggtgttgccg cgggcacgac cctcggtctg
     4861 tcatttcgga aagaacgcac gcttctttat gacgccgcta ctgaacgcct gttgccgggc
     4921 gctgctgcca cactttctgc gagtggagag ggtcatggct gaaatccagc tacgcaatgt
     4981 atcgaagcgc ttcggcaaca tcgttgcggt ggacgacctg tcgatcgacg tgaaagacgg
     5041 cgaattcgtc gtgctgctcg gaccgagcgg cgcgggcaaa accactacgc tgcgcctgat
     5101 cgcgggactc gaacgtcccg atgccggcga cgtgctgatc gacggcgcgg tcgccaccgg
     5161 cgtgcatcct tccgatcgcg atgtcgcgtt tatttttcag caatactcgt tgtatccgca
     5221 tctgactgtg ttcggcaatc ttgcgtttcc gctgcgttcg ccgcgtcgtc gcagcagcga
     5281 ggaacaggtc cgcgcgcgcg tgcacgcggt cgcgcagctg ctgcatatgg aagcgaagct
     5341 cgacaacatg gcaacgcatt tgtcaggcgg cgagatgcag cgcgtcgcga tcggtcgtgc
     5401 gctcgtgcgc gagccgaaag tgtttctgat ggacgaaccg ctatcgtcgc tcgatgcaaa
     5461 gctgcgcgag gaattgcgta tcgagctgaa acggctgcat cgtacgattg gcgcgaccat
     5521 tctttacgtc acgcatgatc aggtggaagc caccacacta gccgaccgca ttggcattct
     5581 cgaacacggt cgtctggtgc aactcggcac cccgcgcgag gtctacggta atccggtttc
     5641 attgagcgcg gcgcagcggc tcggctcgcc gccgatcaac ctgctgccgc cgacgctgtt
     5701 cgattcagca gcggtgccga ccggcaccgc gaccgtggcg attcgtcctg aagacgtggt
     5761 gctgcacgac ggcgacgcgc gtgatgcgcc tggcacccac cacgcgcgcg acgcactcga
     5821 cctgaaggtg ctcgaatact caccgttgcg tcatctgctg attctggatc gcgctggaac
     5881 ggcggttgtg gcgaccacaa tggccgagcg caatttcagc ccggggcagt tggtgggcgt
     5941 gtcgctgcct gctcgcagcc tcttatattt ccgtgcagac ggcaggagga tcccgacatg
     6001 aacggcaaaa cggtcatgga gtgtatcgac gccgcatacg cagcgctgaa agaacacacg
     6061 gacgaaatcg ccgtactcga ccagcagatc ggcgatggcg atcacatctt caatctgctg
     6121 cgcggcatgg aggccttgct tgcagtgcgt gcggacatcg aagcggaaac cttgggggcc
     6181 gcgttagaac tcgcggcgaa caaggtgctg tcgacggtcg gtggatcatc cgggccgctg
     6241 ttcttttcgt tgctgaacgg gatggcgaaa gcatcggcga acaatccgct ggatgttgcg
     6301 ggcaccgcgc tcatgtttgt ggcgggcgtc gaagcggtcg gacagcgggg taagacgggc
     6361 gtaggcagca agacgatgat ggacgtgctg attcccgttg ccgcgcgctt taaggagctt
     6421 gcgaccatcg attcacctac gttgcgaaaa acagtgctgg atgaattgcc gcaggtggcc
     6481 gagcgaaaca tgctcgccac ccgcgatatg ctcgcgacca aagggcgggc atcgtttctg
     6541 ggcgaacgct cgcgcggtca tatcgaccct ggtgcgcgtt ccagtcagat catgatcacc
     6601 gccgtatgcg cgtatctggc gcgcaaccgg gcgtgaacgg aggccagacc atgaaaaaat
     6661 tcatcaacca cgtcgatgat ttcctcgccg aaagtctggc gggatttgca tccgcgcata
     6721 gcgacctggt cgtactcaac aacgaaccgg tgtatgtgcg tcgcaagacg cttaaaccgg
     6781 gcaaagtggc attgatatcg ggcggcggtt cgggacatga gccgttgcac tccggttttg
     6841 tgggctatgg aatgctcgac gcagcctgtc ctggtcaagt cttcacctca cctacaccag
     6901 atcagatgat ggccgctgca gacgctgtcg ataccagtgc gggggtgcta ttcatcgtga
     6961 agaattattc gggtgacctg atgaacttcg aaatggcatc ggagatgtcc gagatcccaa
     7021 gcgcgatggt gctgatcaac gatgacgtgg cagtggaaaa ctccagctac accaccgggc
     7081 ggcgcggtgt cgccggcgtg gtgatcgttg agaagctggt aggcagcatg gccgaaagcg
     7141 gcgccgacct tgaacagtgc aaggcgttcg gcgaccggat caacaagcgc accgcttcga
     7201 tgggggtggc gttcagcagt tgcaccgtgc cggccgcggg aacgctgacg ttcaagattg
     7261 gcgatgatga gatcgaagtg ggcgtgggca ttcacggtga gccgggccgg cgccgcgcaa
     7321 agttctcagg cgccgatgcg attgcgggag agttgctcaa agcaatcgtc gatgacctca
     7381 agccccaagc cggggctgaa ttgctggtgc tcatcaacgg ccttggtgga acaccgcttg
     7441 gcgagcttta cctgctcttc aactcgacgc gcgcgtggct tcagcaacgc gacctgaagg
     7501 tcggccgggc acaggtgggt gcgctgacga cttcgctcga gatggcgggc gcgtcgatta
     7561 cattatgtgt actggacgac gaaatggcac gccactggga cagcccggtc catacgccgt
     7621 cgttgcgttg ggggatgtag ggcgcgctgg cggcgagaaa cgcgggggat acggccacgg
     7681 cgctgcgctg gaacttcacc gcgaagcggg ccgaggcggc ggccccctcg accactgctt
     7741 ctcgtggcga gcgttcagcc agcgccgcga gtaatgggtt cctggcgatc gccgcggccg
     7801 gcgggtgccg cgcgtcctgc cttgcgccgc ctgcacgtca gcgtacatac ttgacatatg
     7861 tctgaccgat cctgctcaat atcctggcag tgtttccctg tgccggcccg catactcgac
     7921 gcgaggcccg gatgccgggc gctgcgatcg aaaccctaag gagataaccg ttgaccacct
     7981 tcctgctacg taatggcgca cttctcgatc ctacccgtcc tgatttgctg gaaggcttcg
     8041 aagtcctcgt cgaggacggc ttcgtgcgcg aagtttctga caaaccgata acaagccgca
     8101 acgcgcatgt gatcgacgtc aaaggcaaaa cgatcatgcc gggtctgatc gatctgcacg
     8161 tgcacgtcgt cgcgatcgag ttcaacctgc cgcgcgtcgc gacgctgccc aatgtgctgg
     8221 tcacgctgcg cgccgtgccg atcatgcgcg ccatgctgcg ccgcggcttc accaccgtgc
     8281 gtgatgcagg cggcgctggc tatccattca agcaggcgct cgagtcaggg cttattgaag
     8341 gcccgcgcct gtttgtctcc ggccgcgcgc tgagccagac gggcggtcat gccgacatcc
     8401 gcgcccgctc cgactacatg ccgtccgatt caccctgcgg ctgctgtgtg cgcgtgggcg
     8461 cgctcggccg cgtggcggac ggcgtcgatg aagtgcgccg ggccgtgcgc gaggaactgc
     8521 agatgggcgc cgaccagatc aagatcatgg cctcgggcgg cgtggcttca cccaccgacc
     8581 ccgtcggcgc gttcggttac tccgaggatg agatccgcgc catcgtcgct gaagcagcgg
     8641 ggcgcggcac ctatgtgctg gcgcatgcct atacgcctgc tgccatcgcg cgcgcggtgc
     8701 gttgcggcgt gcgcacgatc gaacacggca acctgatcga cgaggagacc gcccgcctcg
     8761 tcgcggagca cggcgcgtat gtcgtgccga ccctcgtcac gtacgacgcc cttgcaagcg
     8821 aaggcgagaa atatgggctg ccgccggcca gcattgccaa gatcgcggac gtgcatggcg
     8881 ccggcatgca ctcgatcgaa atcatgaagc gcgccggcgt caagatgggc ttcggcacgg
     8941 acttgctcgg cgaggcccag cgtctgcaga gcgacgaatt ccggcttctg gccgaagtgc
     9001 tctcgccggc cgaggtgatt gcgagcgcaa ccatcgtggg cgcggaagtg ctcggcatgc
     9061 aggacaagct cggcagaatc atgccgggcg cgcatgccga tctgctggtg gtcgatggca
     9121 atccgctgaa gtccgtcgat tgcctgttag ggcagggcga gcatattccg ctcgtcatga
     9181 aggatggccg gctcttcgtc aacgagctcg aaagcagttg accgcatgac ccgcatgccc
     9241 ctggatcgca gcgccacgca gttcctctcg gagtctgtcc atgtcgatga ttgccgcccg
     9301 catcgagcgt ctgccgtttt caggttttca ccgccgtctg ctgctgatgg gcggcctcgg
     9361 ctacaccttc gacgcaatgg acgctgccgt gctcgcgttc ctgctgccgg tgctgcgcac
     9421 gcaatgggg
//
LOCUS       DATASH010001073         2453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6315489,
            whole genome shotgun sequence.
ACCESSION   DATASH010001073 DATASH010000000
VERSION     DATASH010001073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6315489"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="VHV99_28445"
     CDS             <1..847
                     /locus_tag="VHV99_28445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX3639341.1"
                     /translation="NKGLITAVHYKDPNGNSTRVTGKLFVLAANGIETPKLMLMSTTD
                     KFPHGIGNSSDQVGRNLMDHPGTGVTFLANEPLWPGRGPMEMTSIVNFRDGAFRSDYA
                     AKKLHLSNGVPTMSVTADLLKKGLTGAELDRQIRDRAARTLSINSFHEHLAEPQNRVV
                     PSADHKDALGIPQPEIYYSINDYVKKSAANTHELYAQIASLFGGAEVSFDDTFAPNNH
                     IMGTTIMGSDPRDSVVDADCRTHDHSNLFIASSGVMPTAASVNCTLTIAALSLKLADK
                     LKREI"
     gene            869..2302
                     /locus_tag="VHV99_28450"
     CDS             869..2302
                     /locus_tag="VHV99_28450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX3639342.1"
                     /translation="MTKNTSRVQPADALVHSTRRRRFRVATLAAVFALFALYVAWQEA
                     HGPGARRNDELTITQAHADDASQPADATTTPAATPTAASSDLVKRGEYLARAGDCVAC
                     HTADKARPFAGGLPINTPFGTIVTPNITPDPDTGIGQWSAADFMRAMHEGVGKSGERL
                     YPAFPYAEYTKVTDQDVLAIRAYLNTLAPIHYAPPSNSLKFPFNQRWLMVFWNLFNFT
                     EGRFVPDPQQSAEWNRGAYLVQGLAHCEECHTPRNFTQGLKSNTRFSGAVQAGWHAFN
                     ITPDKISGVGDWSDDELVTYLATGAAAGRANAAGPMGEVVADSTQYMTPFDLRSIVTY
                     LRTVPPVSGGESRPRHQWGNPADDVTALRGTAISGVNGAQLFVANCATCHSWTGQGVG
                     ASAPGAYPSLIHNSTVGASDASNLALVILHGVSRTTAHADVLMPAFGPQLSDAQVAAI
                     TNYVTQQFGNPHSTLTPDQVATLRTQQ"
BASE COUNT          484 a          817 c          757 g          395 t
ORIGIN      
        1 taacaaaggg ctcatcacgg ccgtccacta caaggacccg aacggcaaca gcacacgcgt
       61 gaccggcaaa ctgttcgtgc tcgcggcgaa cggcatcgaa acgccgaagc tgatgctgat
      121 gtcgaccacc gacaaattcc cacacggcat cggcaacagc tccgaccagg tgggccgcaa
      181 cctgatggat catcccggca cgggcgtgac cttcctcgcc aacgaaccgc tgtggcccgg
      241 acgcgggccg atggagatga cctcgatcgt caacttccgc gacggcgcat tccgctccga
      301 ttacgcggcg aaaaaactgc atctgtcgaa cggcgtgccg accatgagcg tgaccgccga
      361 tctgctgaaa aaaggcctca cgggcgccga gctcgaccgc cagatccgcg accgcgcggc
      421 ccgcacgttg agcatcaaca gctttcacga gcatctcgcg gagccgcaga accgcgtggt
      481 gccctcggcc gatcacaagg atgcgctcgg cattccacag cccgagatct attactcgat
      541 caacgactac gtgaagaaga gcgcggcgaa cacgcacgag ctgtatgcgc agatcgcgtc
      601 gctgttcggc ggcgccgagg tctcattcga cgatacgttc gcgccgaata atcacatcat
      661 gggtaccacc atcatgggca gcgatcctcg ggattcggtc gtcgacgctg actgccgcac
      721 gcacgatcat tcgaatctgt tcattgccag cagcggggtg atgccgactg ctgcctccgt
      781 gaattgcacg ctgacgatcg cggcgttgtc gctgaaactg gccgacaagc tcaagcgtga
      841 aatctgagcc ctgaacggag tgcccccaat gacgaagaac acctctcgcg tgcagccggc
      901 cgatgcgctt gtccacagca cgcggcgtcg ccgcttccgg gtggcgacac tggcggcggt
      961 atttgcgctg tttgcgctgt atgtcgcctg gcaggaagcg catgggcctg gcgcgcgacg
     1021 caacgacgag ttgacgatca cgcaggccca tgcggacgac gcctcgcaac cggccgatgc
     1081 gacgaccaca cccgcggcta caccgacagc ggccagcagc gatctcgtga aacgcggtga
     1141 atatctcgca cgggccggcg attgcgtcgc ctgccacacg gctgacaaag cacgcccatt
     1201 cgcgggcggc ctgccgatca acacgccgtt cggcacgatc gtgacgccga acatcacgcc
     1261 cgacccggac acgggcatcg gccaatggag cgccgccgat tttatgcgcg ccatgcatga
     1321 aggcgtcggc aagagcggcg agcggcttta tccggccttc ccatatgcgg agtacaccaa
     1381 ggtcaccgat caggacgtgc tggcgattcg cgcgtatctg aacacgctcg cgccgatcca
     1441 ctacgcgccg ccgagcaaca gcctgaaatt cccgttcaac cagcgctggc tgatggtgtt
     1501 ctggaacctg ttcaatttca ccgaaggccg cttcgtgccc gatccgcaac aaagcgccga
     1561 gtggaatcgc ggcgcgtatc tggtgcaagg cctcgcgcat tgcgaggagt gccacacgcc
     1621 gcgcaatttc acgcagggct tgaagtcgaa cacgcgtttc tccggcgcgg tgcaggccgg
     1681 ctggcatgcg ttcaacatca cgccggacaa gatcagcggc gtaggcgact ggagcgatga
     1741 cgagcttgtc acgtatctgg cgaccggcgc ggcggcgggc cgcgcgaatg cagccggtcc
     1801 gatgggcgaa gtggtcgccg acagcacgca gtacatgacg ccgttcgatc tgcgttcgat
     1861 cgtcacgtat ctgcgcacgg tgccgccggt cagcggcggc gagtcgcgcc cgcgccatca
     1921 gtggggcaat cccgccgacg acgtgaccgc gctgcgcggc accgcgatca gcggggtgaa
     1981 cggcgcgcag ctttttgtgg ccaattgcgc gacctgccat agctggacgg gccagggtgt
     2041 gggcgcgagc gcgccgggcg cgtatccgtc gctgatccac aattcgacgg tcggcgcgag
     2101 cgacgccagc aatctcgcgc tggtgatcct gcatggcgtg agccgcacga ccgcgcacgc
     2161 ggacgtgctg atgcccgcgt tcggcccgca actcagcgac gctcaggtgg ccgccatcac
     2221 caactacgtg acccagcagt tcggcaatcc ccactcgacg ctaacacccg accaggtcgc
     2281 cacgctgcgc acgcagcaat agtgagcgcc aaggacgtgt ttcccgtagg tcattgcggg
     2341 aacacgccac tttcgagtca ttttgtgtca ctttcttgcc gacaacgcgc caggatacga
     2401 tgggcaccgc gcatacgtag ccgcgcgcgc cgcactatca tgagcgaatg gct
//
LOCUS       DATASH010001074         2009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2364339,
            whole genome shotgun sequence.
ACCESSION   DATASH010001074 DATASH010000000
VERSION     DATASH010001074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2364339"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..1089)
                     /locus_tag="VHV99_28455"
     CDS             complement(217..1089)
                     /locus_tag="VHV99_28455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX3639343.1"
                     /translation="MQNLLAEIHSNRWAFLWDALTTISMHLCAAALILVVGWWLARRV
                     ARSLNRLLASQSRMDATLRPVICDTCLWSIRVVSIIGALSQLGIQTASIVAVLGAAGL
                     AIGLALQGTMQNIAAGIMLLLLRPFKVGDSIDAGTGNVSGTVEEISLFTTRLTKSDGI
                     CEYVPNSALWSNSIRNYNRNPTRRLDLEVEISVRDDVDQALAALRNLAVADPRALQTP
                     APQVMVARFDDSTVVLNIRVWAHIDTFWDMRWDLARQVRQTLNDAQCGLPVRTRELHI
                     VQNNETDESAAARV"
     gene            complement(1465..>2009)
                     /locus_tag="VHV99_28460"
     CDS             complement(1465..>2009)
                     /locus_tag="VHV99_28460"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3639344.1"
                     /translation="PVVRDATEADLPAIQAIYAHHVLTGVASFEEIPPSVDDLRTRLA
                     SVRSHGLPYMVAEIDGEVAGYCYATPYRPRAAYRNTIEDSIYIGDAYRGRGLGRVLLL
                     ALIERCETGPWRQMVAVIADGGSGGSLSLHTQLGFELTGTLKAVGFKHGRWLDTTLMQ
                     RRLGAGASSVPDDVTQQERD"
BASE COUNT          376 a          592 c          678 g          363 t
ORIGIN      
        1 ccgcggacct ggcggtggat gaagacggcc tcgtgatcga ctatccgacg ctattcacgc
       61 gcctgccgcg cgagcgttga acatcgaggt gccggaaccg cggctgtctg ctggcggctg
      121 ttcccggtgc cgctctcgac aaagcgttcg atgcctgccg caatacgctc gggttcgcgc
      181 gacacgaccc aatgcccggc gtcgatttca gtgcggctat acgcgagccg cggcggattc
      241 gtcggtctcg ttattttgca cgatatgcag ttcacgtgta cgcaccggca agccgcattg
      301 cgcgtcgttg agcgtttgac ggacctggcg ggcgaggtcc cagcgcatgt cccagaacgt
      361 gtcgatatgg gcccacacac ggatgttcag gaccacggtg ctgtcgtcga aacgggccac
      421 catcacctgc ggcgcgggcg tctgcagcgc acgcgggtcg gcaacggcga gattgcgcag
      481 tgcggccagc gcctggtcga cgtcgtcacg cacggagatt tccacttcca gatcgaggcg
      541 gcgggtcgga ttgcggttgt aattgcggat tgaattgctc cacagcgcac tgttcggcac
      601 gtattcgcaa atgccgtcgg atttcgtgag acgcgtggtg aacaggctga tttcctcgac
      661 ggtgccggag acgttgcccg tgccggcgtc gatcgaatcg cccaccttga acggccgtaa
      721 aagcaaaagc atgatgccgg cggcgatgtt ttgcatcgtg ccctgcagcg cgaggccgat
      781 agcgaggccg gcggcgccga gcacggcgac gatgctcgcc gtctggatac ccagttgcga
      841 cagcgcgccg atgatggaga cgacgcgaat gctccacaag caggtgtcgc agatgaccgg
      901 gcgcagcgtg gcgtccatgc gcgactggct tgcgagcagc cggttgagtg agcgggcgac
      961 gcggcgggcg agccaccagc ccaccaccaa tatcaatgcg gccgcgcaca ggtgcatcga
     1021 aatcgtggtg agtgcgtccc aaagaaaggc ccaccggttg gagtggatct cagcgagtag
     1081 attctgcatg gccttgacgg tcctgatttt caagcggcgg cgagttgttc gccgcatggt
     1141 ttgtaggaag cctgaacacc gcgcgcccgc gtagcgggag tgacgagcag gtgttcggaa
     1201 catgacgcga ttgaagcgtg ctgaaaaggg accgcttcgc aaaaggaaag ttctaccggt
     1261 acacgctttt tgacagattg agtttacggc tgagaagggc ggcggtaacg agacgagggg
     1321 cgtgtcgtca tggccccgtg atggattggg taacggggcc agtgaaatgc ggcgtgacct
     1381 gagaaacgct tcctcaagcc gccgcaacgt tacgacatga gttgatacgg atggcgaact
     1441 aatctgtcgc gccgtgtgat tcattcagtc acgttcctgc tgtgtcacat cgtccggtac
     1501 ggatgaagcg cctgcgccca ggcgccgctg catcaacgtg gtatcgagcc agcggccatg
     1561 tttgaagccg acggccttca acgtgccggt caattcgaag cccaactgcg tgtgcagcga
     1621 cagcgaaccg ccgctgccgc cgtcagcaat cacggcgacc atctggcgcc acgggccggt
     1681 ctcgcagcgt tcgatcaggg ccagcaggag cacgcggccc aagccgcgcc cgcgatacgc
     1741 gtcgccgatg tagatcgaat cttcaatcgt gttgcgatag gcggcgcgtg gccggtacgg
     1801 cgtggcgtag caatagccgg cgacttcgcc gtcgatctcg gccaccatgt acggcagtcc
     1861 atgactgcgc accgaagcga ggcgcgtgcg caggtcgtcg acggaaggcg ggatttcttc
     1921 gaacgaggcg accccggtca gcacgtggtg cgcatagatc gcctggatcg cgggcagatc
     1981 ggcttcggtg gcgtcgcgga ccacgggcg
//
LOCUS       DATASH010001075         3584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5329414,
            whole genome shotgun sequence.
ACCESSION   DATASH010001075 DATASH010000000
VERSION     DATASH010001075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5329414"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2033
                     /locus_tag="VHV99_28465"
     CDS             <1..2033
                     /locus_tag="VHV99_28465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX3639345.1"
                     /translation="SVVGGLYASGMTEQDMQNRLAGINLADIAFDVTERADLPQSRRE
                     GERLYVNSLSLGYGTNGFRLPVGLIQGNRLQALLQDWTSAAPGNVSFNRLPIPYRAIA
                     TDLRTGQKVVLDHGSLPQAIRASMALPGLFAPADIDGRTLVDGGIVSNLPIETARDMG
                     ANVVIAVDIGSPLRPLDALASPADVMQQMIGILIHQNVARQREQLQRGDVLIEPALGS
                     LSFTDFANASQAIAAGAAATRAAMPQLQQLALSPAAFAAWRARHSVPAMRPVRITQIE
                     IASQGAVPEARIRAALHVKPGDVYDPAALNKDLLALSTTGDFDSVTQQLVDDGDEHKL
                     IIVAQQKQWGPNFLLFGLGLSSSSTDEGGFRLHLGYRRPWLTSSGLEGRVDTTVGSDL
                     IDLHAELRQPLSGSFGYYVAPYLEFQRRYANIYDDSGNVKLTQYRLQTERAGLDFGLP
                     LARLGDFRIGVAYAHGFASPQYNLPIDEGPQSPLLFPDIYGRQLSARARLVIDQLDDP
                     LFARKGYFGELRVERSLFAGSDSFTEVYGKGIVAASYGRHSINASIEAGNDFGGPNLT
                     NPFGFTLGGFQHLSAYAADQLSGNSMLYGQLTYMNQLATFNASPIHGLFAGLSVEAGN
                     VWNRDSQFGSGSLKRSVTVFTALTSSFGPVYLGVAFAPGGRNNFYFQLGHAY"
     gene            complement(2056..3183)
                     /locus_tag="VHV99_28470"
     CDS             complement(2056..3183)
                     /locus_tag="VHV99_28470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3639346.1"
                     /translation="MPPPAEQKKLTPYRYAKYRWHHFRRSWTDTRADRIPSWSRLYLR
                     VYTRLLTLTILVLAASSATLTLTQKTPSIWQTLTTAKASIPIALVTLLLPALTAHRWM
                     RPVWLDLVMVRERAIDFTGGRFNTRARESHSVIIGPLARTLNALAERMERLIAAQRDL
                     TNGISHELRTPLARVRFALESLREPASAAEYNSALASIGQDVTELDELIDMSLTYARL
                     EYSSLQSNLEATLLAVWFDSQITDATLLYADKQLASQVDVDTSLRVVMDKRLMSYAMR
                     NVLRNAARYAHSRIVVGLSIKHGNVEIYVEDDGTGVPPDQRERIFNAFVRLDRQTGGY
                     GLGLAITQQVLRAHHGRIAVTDPLTLSGARFEMSWPAGSAA"
BASE COUNT          606 a         1069 c         1134 g          775 t
ORIGIN      
        1 gctccgtggt cggcggcctg tacgcgagcg gcatgacgga gcaggatatg caaaaccgcc
       61 tcgcggggat caatctcgcg gacatcgcgt tcgacgtcac cgaacgcgcc gatctgcccc
      121 agtcacgccg cgagggcgag cggctgtatg tgaacagcct gtcactcggt tacggcacca
      181 atggcttccg gttgccggtc ggactcatcc agggcaatcg cttgcaagcg ctgctgcaag
      241 attggacgtc cgcggcgccg ggcaatgttt cgttcaaccg actgccgatc ccgtatcgtg
      301 cgatcgccac cgatctccgc accggtcaga aggtggtgct ggatcacggt tcgctgccgc
      361 aggcgatacg cgccagcatg gcgttgcccg gcctgttcgc accggccgat atcgacggac
      421 gcacgctggt ggacggcggc atcgtcagca acctgcccat cgaaacggcg cgcgacatgg
      481 gcgcgaacgt cgtgatcgcg gtcgatatcg gctcgccgct gcggccgctg gacgcgctgg
      541 cctcgcccgc ggacgtgatg cagcagatga tcggcatcct gatacaccag aacgtcgcgc
      601 ggcaacgtga acagctgcaa aggggtgacg tcctgatcga gccggcgctc ggctcgctga
      661 gcttcaccga tttcgccaac gccagccagg cgatcgcggc cggcgcggcg gccacgcgcg
      721 cggccatgcc gcagcttcag cagctggcgc tctcgcccgc cgctttcgcc gcatggcgcg
      781 cccggcatag tgtgcccgcc atgcgtccgg tacggatcac gcagatcgag atcgcgtcgc
      841 agggcgccgt gcccgaggcg cgcattcgcg ctgcgttgca cgtcaaaccc ggcgacgtgt
      901 acgatccggc cgcgctcaac aaggatctgc tggcgctatc caccacgggc gacttcgata
      961 gcgtcaccca gcaactcgtc gacgacggcg acgagcacaa gctcatcatc gtcgcgcagc
     1021 aaaaacaatg gggaccgaat ttcctgctgt tcgggctcgg tttgtcgagt agttcgacgg
     1081 atgaaggcgg cttccggctg catctcggtt atcggcggcc atggcttact tcgtcagggc
     1141 tcgaagggcg agtggacacg acggtcggca gcgacctcat cgaccttcat gcggaattgc
     1201 ggcaaccgct gtcgggttcc ttcggctatt acgtggcgcc ctacctcgag tttcagcgcc
     1261 gctatgccaa catctacgac gactccggca atgtcaagct cacgcaatac cgcttgcaga
     1321 ccgagcgcgc gggactcgat ttcggcttgc cactcgcgcg tctcggcgac ttccgtatcg
     1381 gggtggcgta cgcgcatggc ttcgcgtcgc cccaatacaa cttgcccatc gacgagggtc
     1441 ctcagtcgcc gttgctgttt cccgatatct acgggcggca gctcagcgcg agagcgcgtc
     1501 tcgtgatcga tcagctcgac gatccgctgt ttgcgcgtaa aggttacttc ggcgaactac
     1561 gcgtggagcg ttcgttgttc gcgggcagcg acagcttcac cgaggtctac ggcaaaggca
     1621 tcgtcgcggc gagctatgga cggcacagca tcaatgcgag catcgaagcg ggtaacgact
     1681 ttggcggccc caacctgacc aatccgttcg gcttcacgct gggcggtttc cagcatttgt
     1741 cggcctatgc cgccgaccag ctgtccggca attcgatgct gtatggccag ctcacgtaca
     1801 tgaatcagct tgccaccttc aacgcgtcgc cgattcacgg cctgtttgca gggctgagcg
     1861 ttgaagccgg caacgtctgg aaccgcgatt cgcagttcgg gagcggatcc ttgaagcgca
     1921 gcgtgacggt tttcaccgcg ctgaccagtt cattcggacc ggtatatctg ggcgtagcgt
     1981 tcgcaccggg cggccggaat aacttctact ttcagcttgg acatgcctat tgaagcggcg
     2041 tggagatgat gcgtttcacg ccgcactccc cgccggccag ctcatttcga aacgtgcgcc
     2101 gctcaacgtc aatggatcgg tcacggcaat gcgtccgtgg tgagcgcgca gcacctgttg
     2161 cgtgatcgcg agacccaaac cgtatccgcc ggtttgacga tcgagccgca cgaaggcgtt
     2221 gaaaatgcgc tcgcgctggt cgggcggcac gcctgtgccg tcgtcttcca cgtagatttc
     2281 cacattgcca tgtttgatgc tcagaccgac cacgattcgc gaatgggcgt atctggccgc
     2341 gttgcgcaat acgtttcgca ttgcgtagga catgaggcgt ttgtccatta ccacgcgtaa
     2401 cgatgtgtcg acgtcgactt gcgaggcgag ttgcttgtcc gcgtaaagca acgtggcgtc
     2461 agtgatctga ctgtcgaacc ataccgcgag caaggtggct tcgagattcg attgcagcga
     2521 gctgtattca aggcgcgcgt aggtcaggct catgtcgatg agttcgtcga gttcggtaac
     2581 gtcctggccg atgctcgcca atgcgctgtt gtattcggcg gcggatgcgg gttcgcggag
     2641 gctttcaagt gcgaagcgca cacgggctaa gggcgtgcgg agctcgtggg agatgccatt
     2701 ggtgaggtcg cgctgtgcgg cgatcaggcg ctccatgcgc tcagccaggg cgtttaacgt
     2761 gcgggccagt gggccgatta tgacgctgtg cgattcgcgc gcgcgggtgt tgaaacggcc
     2821 gcctgtgaag tcgattgcgc gttcgcggac catcaccagg tctagccaga ctgggcgcat
     2881 ccagcggtgg gctgttaggg ccggtagcag tagggttacc agtgctattg ggatcgatgc
     2941 ttttgctgtt gttagtgttt gccagattga tggcgttttt tgtgtcaggg ttagtgttgc
     3001 tgatgatgct gctagcacta gtattgtcag cgtcagcagg cgcgtgtaga cgcggaggta
     3061 tagcctcgac cagctcggga ttctgtccgc tcttgtgtct gtccacgagc ggcggaaatg
     3121 gtgccagcgg tattttgcat agcggtaagg ggttagcttt ttttgctccg ctggaggtgg
     3181 catttctttc tctttcgctt cttttgttgc ggttgtggtc tattagtgtt gcccctgtgc
     3241 ggggcggcac ttactttctt tgccgccgca aagaaagata agcaaagaaa gcggctcacc
     3301 ccgccagcgc ataagcgggg cccccgcgca gtcgcggtag tggtgcatct ggaatccggg
     3361 ttctcgcaca tttggcttca gtgacaaggc agtcttactt cccgcctcgc actgcgtgct
     3421 cgccggaacg ggacaccggg aagactgcgg cttcgcttgg tgtacttcgc ctcgtactgc
     3481 gtgctcgccg cataggttca tccggcttgc tccgcggtcg ggctccttcg cgtatcgcct
     3541 gacgtttcac tttgatcagg tttgcgtttt tttctgtcct ttcg
//
LOCUS       DATASH010001076         3522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_778522,
            whole genome shotgun sequence.
ACCESSION   DATASH010001076 DATASH010000000
VERSION     DATASH010001076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778522"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..2493)
                     /locus_tag="VHV99_28475"
     CDS             complement(97..2493)
                     /locus_tag="VHV99_28475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX3639347.1"
                     /translation="MNAPETHHLIGASVERKEDYRFLTGNGQYTDDIVLPQQTYAVFL
                     RSPHAHAKINSIDTAAAKQSPGVIAIFTGADMATDNVGGLPCGWLIHSTDGKPMNEPP
                     HPIIAHTKVRHVGDQVALVIADSIKAAKDAAELIEVDYDVLPAVVDTAHAADPGQAAV
                     HDEVPDNVCYNWGHGDKAATDAAFAKAAHVTTLDIVNNRLIPNAIEPRAVNASYSVQD
                     ESYTLYVANQNPHVERLLMAAFVLSLPESKLRVIAPDVGGGFGSKIFLYAEDVALTWA
                     SKKIRRPVKWTAERSEAFVSDAHGRDHVTKAELAMDADGKFLAMRIHTIANMGAYLST
                     FASSVPTILYATLLAGQYTTPAIYAEVKAVFTNTVPVDAYRGAGRPEATYVVERLVET
                     AAREMKLDPAEIRRRNFIREFPYATPVGLTYDTGDYETVLARSLELADVKGFEARRQE
                     SEKNGKRRGLGYSCYIEACGLAPSNIAGALGARAGLFEVGQIRVHPTGSVTVFTGSHS
                     HGQGHETTFAQVVADRLGIALESVEIVHGDTGRIPFGMGTYGSRSIAVGGSAIMKALD
                     KIETKAKKIAAHLLEAAAEDIEFKDGVFRVAGTDRTKAFADISLAAYVPHNYPLDVLE
                     PGLEESAFYDPTNFTYPSGAYICEIEVDPDTGVCRIQQFTAVDDFGNVINPMIVEGQV
                     HGGLAQGIGQAMLERCVYDNESGQLLSGSYMDYAMPHASDLPNFTVETVKGTPCTHNP
                     LGVKGCGEAGAIGSPPAVINAILDALAPLGVTDLQMPATPHRVWSAIHAANSPAKQAH
                     "
     gene            complement(2504..3031)
                     /locus_tag="VHV99_28480"
     CDS             complement(2504..3031)
                     /locus_tag="VHV99_28480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX3639348.1"
                     /translation="MAISISLTVNGAPISASIDPGTLLVQFLRDQLRLTGTHIGCDTA
                     QCGACTIHLNGRAIKSCNILAVQAEGTNITTIEGLARDGALHPMQTAFKHCHGLQCGF
                     CTPGMVMSAVSLVERQPNLTGDDVRAQLDGNLCRCTGYHNIVKAVLEGAEGMKSSGTA
                     SAPATPAAATAPVTA"
BASE COUNT          542 a          973 c         1218 g          789 t
ORIGIN      
        1 gcgtcgctgt cagcggtgag ggctgcggcg gccgctttgg gatccgtcgc gcgttgatac
       61 tcgaatgaat acatgtcgaa tgctccgctc aggatctcag tgggcttgct tggcaggtga
      121 attggctgcg tgaatcgcgg accacacgcg atgcggcgtc gccggcattt gcaggtcggt
      181 cacgccgagc ggcgcgagcg cgtcgaggat cgcgttgatg accgccggtg gcgagccgat
      241 cgcgcctgcc tcgccgcaac ccttcacccc gagcggattg tgcgtgcacg gcgtgccctt
      301 cacggtttcg acggtgaagt tcggcagatc ggacgcatgc ggcatcgcgt agtccatgta
      361 ggagccggac agcaactggc cactttcgtt gtcgtacacg cagcgttcca gcatcgcctg
      421 accgatgcct tgagcgagtc cgccgtgcac ctggccttcg acgatcatcg ggttgatcac
      481 gttgccgaaa tcgtcgaccg cggtgaactg ctggatgcgg cacacgcccg tgtcgggatc
      541 gacctcgatc tcgcagatat acgcgccgga tggatacgtg aagttggtcg gatcgtaaaa
      601 cgcgctttct tcgagacccg gttcgagcac gtcgagcgga tagttgtgcg gcacatacgc
      661 cgcgagcgag atgtcggcga aggctttggt gcgatcggtg cccgcgacgc ggaacacgcc
      721 gtccttgaac tcgatgtctt ccgctgcggc ttcgagcaga tgcgcggcga ttttcttggc
      781 cttggtttcg atcttgtcga gtgccttcat gatcgccgag ccgccgaccg caatcgagcg
      841 cgaaccatac gtgcccatgc cgaacggaat gcggccggtg tcgccgtgaa cgatctcgac
      901 gctttccagt gcaatgccga gccggtccgc gaccacttgc gcgaaggttg tttcgtggcc
      961 ttggccatga ctgtgcgagc cggtgaacac ggtgacggaa cccgtggggt gcacacgaat
     1021 ctggccgact tcgaagagtc cggcgcgtgc gccgagcgcg ccggcgatgt tcgacggcgc
     1081 caaaccgcag gcttcgatgt agcacgagta gccgaggccg cgtcgcttgc cgttcttctc
     1141 cgattcctgc ctgcgggctt cgaagccttt cacgtccgcg agttcgagcg agcgtgcgag
     1201 gacggtttcg tagtcgccgg tgtcgtaagt gaggccgacc ggtgtggcat acggaaactc
     1261 gcggatgaaa ttgcggcggc ggatttccgc gggatcgagt ttcatctcgc gtgccgcggt
     1321 ttcgaccagg cgctcgacca cataggtcgc ttcgggacgg cctgcgccac ggtaggcatc
     1381 gaccgggacg gtgttggtga agaccgcttt cacctcggcg tagatggcgg gcgtggtgta
     1441 ctggccggcg agcagcgtgg cgtacaggat ggtcggcacg ctcgaggcaa aggtcgacag
     1501 atacgcgccc atgttggcga tcgtatggat gcgcatcgcg aggaatttgc cgtcggcgtc
     1561 catcgccaat tcggctttgg tcacgtgatc gcggccgtgc gcgtcggaca cgaaagcttc
     1621 cgagcgctcg gcggtccatt tgaccggacg acgaatcttc ttcgacgccc acgtcagcgc
     1681 gacatcttcg gcgtacagga aaatcttcga cccgaagccg ccgccgacat ccggcgcgat
     1741 gacgcgcagt ttcgattccg gcagcgacaa cacgaacgcg gccatcaaca ggcgttccac
     1801 gtgcggattc tggttcgcga cgtagagcgt atagctctcg tcctgcaccg agtagctcgc
     1861 gttgaccgcg cgcggctcga tggcgttcgg gatcaggcgg ttattgacga tgtcgagcgt
     1921 ggtgacgtgc gcggctttgg cgaaggcggc gtcggtcgcg gctttgtcac cgtggcccca
     1981 gttgtagcag acgttgtccg gcacttcgtc gtgaaccgcg gcctggcctg gatcggccgc
     2041 gtgggcggtg tcgaccacgg ccggcagcac gtcgtaatcg acctcgatca gttcggcggc
     2101 gtctttggcg gccttgatcg aatcggcgat gacgagcgcg acctgatcgc cgacatggcg
     2161 cactttcgta tgcgcaatga tcggatgggg cggctcgttc atcggcttgc catcggtact
     2221 gtggatcagc cagccgcacg gcaggccgcc gacgttgtca gtggccatgt ccgcgccggt
     2281 gaagatcgcg attacgccgg gcgactgttt ggccgcggcg gtatcgatgc tgttgatttt
     2341 tgcgtgcgcg tgcggcgagc gcaggaagac cgcgtaggtt tgctgcggca ggacgatgtc
     2401 gtcggtgtac tgaccgttgc cggtgaggaa ccgatagtct tccttacgtt cgacggaggc
     2461 gccgatcagg tgatgggttt cgggtgcgtt catcgtcgtc tcctcaggcg gtaacgggcg
     2521 cggtggctgc ggccggggtc gcgggggcgc tggccgtgcc ggaggatttc atgccttcgg
     2581 cgccttcgag cactgctttg acgatgttgt gatatcccgt acagcggcac aggttgccgt
     2641 cgagttgggc gcgcacgtcg tcgccggtga gattgggctg acgttcgacg agtgagaccg
     2701 cgctcatgac catgccgggc gtacagaagc cgcactgcag gccgtggcag tgtttgaagg
     2761 cggtttgcat cgggtgcagg gcgccgtctc tggcaagccc ttcgatggta gtgatgtttg
     2821 tgccttcggc ctggaccgcg agaatgttgc aggatttgat cgcgcggccg ttcaggtgga
     2881 tggtgcaggc gccgcactgg gcggtgtcgc aaccgatgtg cgtgcctgtg aggcgaagtt
     2941 gatcgcgcag gaactggaca agcagggtgc caggatcgat tgaggcgctg atgggcgcgc
     3001 cgttcaccgt cagactgatg ctgatcgcca tgaagttgtc tccttatggg gtgagaccgg
     3061 acctttttct tgccttcggc tgcctgttcg ttaggtccga tgagggtgat gctggtttgt
     3121 ggcttttttt atgggtctgc cgaaaagtaa agcacaaatt gggtgggggc gctattgggt
     3181 tgtgcctacg gcgttggggt ttggttgggc ttttggctgc gcggtgcttg ttgtctggtt
     3241 tgcctgcggc ggtgggcttt gcttgatttg gttgcctgtt cggcggtttt tgtctgtgtt
     3301 tttgtggtgt tgggtttttt cttgatttgg ggtggttgct cgttcgtggt tctgtttgcc
     3361 tgcggtgttg gcctttcctt gaattgatat tggtctatta gcgtcgcccc tgtgcggggc
     3421 aggcacctac ttttctttgc ctgccgcaaa gaaaagtagg caaaagaaag cgggctcaaa
     3481 ccgccagctt ataagcgggt cccccgcaca gccgcggtag tg
//
LOCUS       DATASH010001077         1594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4738239,
            whole genome shotgun sequence.
ACCESSION   DATASH010001077 DATASH010000000
VERSION     DATASH010001077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4738239"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /gene="argA"
                     /locus_tag="VHV99_28485"
     CDS             <1..570
                     /gene="argA"
                     /locus_tag="VHV99_28485"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015602335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HEX3639349.1"
                     /translation="SIRACRGGVARAHIIPYALDGSLLLELFLHDGVGTMISYENLES
                     LREATPDDVGGILSLIEPLESDGTLVRRGRHQIERDIDHFSVIEHDGVLFGCAALYPY
                     PQERIGEMACLTVSPEAQGTGDGERLLKRIEQRARARGLTRIFVLTTRTEHWFLKRGF
                     VKVTVDDLPEDRRRLYNWQRKSLVLMKQL"
     gene            627..902
                     /locus_tag="VHV99_28490"
     CDS             627..902
                     /locus_tag="VHV99_28490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEX3639350.1"
                     /translation="MTRMVQCAKLGKEAEGLDFPPLPGELGKRIYESISKEAWQAWLK
                     QQTMLINENRLNMADPRARQYLMKQTEKFFFGEGADTAQGYVPPAAE"
BASE COUNT          317 a          529 c          436 g          312 t
ORIGIN      
        1 tcgatccgcg cctgccgcgg cggcgtggcg cgggcgcaca tcatccccta cgcgctcgac
       61 ggcagcctgc tgctcgaact gttcctgcac gacggcgtgg gcacgatgat ctcgtacgag
      121 aacctcgaaa gcctgcgcga agccacgccg gacgacgtcg gcggcattct ctcgctgatc
      181 gagccgctcg aatccgacgg cacgctggtg cggcgcgggc gccaccagat cgaacgcgac
      241 atcgaccatt tctcggtgat cgagcacgat ggcgtgctgt tcggctgcgc ggcgctgtat
      301 ccctacccgc aggaacgcat cggcgaaatg gcgtgcctca ccgtctcgcc ggaagcgcaa
      361 ggcaccggcg acggcgaacg cctgttgaaa cgcatcgaac aacgcgcccg ggcgcgcggc
      421 ctcacgcgca ttttcgtgct caccacgcgc accgaacact ggtttctcaa gcgcggcttc
      481 gtcaaggtca cggtcgacga cctgcccgaa gaccgccgcc ggctttataa ctggcagcgc
      541 aagtcactcg tgctgatgaa acagctctga gcgtccccat ataaagcgac tgcccgcccc
      601 acacttgatt aacggagaag cacagcatga ctcgtatggt tcaatgcgcg aagctcggca
      661 aggaagccga aggcctcgat ttcccgccgc tgccgggcga actcggcaag cggatctacg
      721 aaagtatttc gaaggaagcc tggcaggcgt ggttgaagca gcaaaccatg ctgatcaacg
      781 aaaaccgcct gaacatggcc gatccgcgcg cgcgccaata tctgatgaag cagaccgaga
      841 agttcttctt cggtgaaggc gccgatacag cccaaggcta cgtgccgccg gcagctgagt
      901 aagcgctttc ggttgcacgc cagcattggc gatcagtccg atgaaaaatc cgcgccgtgt
      961 gcgcggattt ttttcgtccg gttttcgttc caaccgtgcc gcaaagactc tccagacggt
     1021 acattttcgg ccagttttaa gccacttctc cgtaccgccg aatcgcgcca aatcccctgc
     1081 cccgcaaggg attgcacacc gtccccaaaa cccgcgccgg ctatcaggtt tgcacgatcc
     1141 tctgtcatcg tgctatcatg ttcaccgttc caacagcaat tcaccttcat tcacgttcac
     1201 atcaggcttg gttaggtagc gttcgcaagc actgctcgca tggttttcac cgcgggttca
     1261 cagcgaattt cagccaggcg gatcgaacag tttttataca agaaggctcg tcggtcgttt
     1321 caccgcttca tctcgcatcg tgagcggcga tccagcaccg gcctttttcg tttcaagcct
     1381 tccagcggcg cacgggccag ctttgagcca ccccagcgtc ctcatgcatc tgcccccctt
     1441 ccacggccac gcagatgcaa cagtcagcac aatttgacga gtgttttttc gcgacctgtc
     1501 tcgcgaggca cgggcgttct gctttccatc gcgcccagct ccatcgccct cagcggcgac
     1561 ggagacgctt ttcgcatggc tcgcgaaact gcac
//
LOCUS       DATASH010001078         2916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2955807,
            whole genome shotgun sequence.
ACCESSION   DATASH010001078 DATASH010000000
VERSION     DATASH010001078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2955807"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1201
                     /locus_tag="VHV99_28495"
     CDS             <1..1201
                     /locus_tag="VHV99_28495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEX3639351.1"
                     /translation="EYGFPESHAASFALLVYLSAWLKRYEPAAFLAGLLNSQPLGFYS
                     PSQLVQDAKRHGVQVLPPDVTLSDWESTFERRGQEGQRISSSETCLHRQQGVLSAQQL
                     RDLSLRRFTVARTIRRAAKRLTARVFQSSNTYGARGPAVRIGMHLIKGLSQVAAERIM
                     AARSEAQFTDVDDLARRAALTRRDLEALAAANALVSIAGHRREAWWAVTAQHTVPKLL
                     RDAPIDEARLALPQAAESREIVDDYASLGLTLNRHPLALLRERLAKQRFRTAAELAAC
                     QHGTLARACGIVTVRQRPGTANGTVFVSLEDETGSINVIVWPLLVEKQRKVLLGSSLP
                     AVYGVLQRDDGVSTGGHGAGNVMNANQAGRAKKKQKGEVIHLVAHRLEDHSEWLGDLA
                     TASRDFH"
     gene            complement(1515..2435)
                     /locus_tag="VHV99_28500"
     CDS             complement(1515..2435)
                     /locus_tag="VHV99_28500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3639352.1"
                     /translation="MFKVAVIGASGLLGRVLVDELARQAGWQILATAFSRPGPNTVGL
                     DIRDAQAVEQFVEREAPDALVIAAAERRPDVCEHDPALARALNVDAVRTLAAAARRRG
                     AWTLSISTDYVFDGTHPPYRHDCAPAPLNAYGRSKLEGERALTDSTDLGCVLRLPLLY
                     GPIVDWAESAVTSLVPAIAASASRDGKAAVMDAWAIRYPTFTPDVAFVIRQMLERHAQ
                     GEAIHGIVQWSGDEPMNKYEIAMRLAQALQLDAQLTPQHMPTGATPRPHNCHLASDRL
                     EALGIGRRTPFDVAIRQVLAGFPWRGETLA"
     gene            complement(2449..2667)
                     /locus_tag="VHV99_28505"
     CDS             complement(2449..2667)
                     /locus_tag="VHV99_28505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEX3639353.1"
                     /translation="MTIEFRVEGMSCQHCVAAVTHAIREHDGAAQVHVDLTSGRVAVE
                     SAQSADTLKTAIDEAGYMVTAVTNGSAR"
     gene            2791..>2916
                     /locus_tag="VHV99_28510"
     CDS             2791..>2916
                     /locus_tag="VHV99_28510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX3639354.1"
                     /translation="MNIGEAARVSGVTAKMIRYYESVGLLAAKARTSAGYRVYGPQ"
BASE COUNT          540 a          903 c          934 g          539 t
ORIGIN      
        1 cgagtatggc tttccggaaa gtcatgcagc aagcttcgca ttgctcgtct atctcagcgc
       61 gtggttgaaa cgttatgagc cggccgcttt tctggcgggt ttgctcaaca gtcagccgtt
      121 gggattctat tcgccgtcgc agctggtgca ggacgcgaag cgtcacggcg tccaggtatt
      181 gccgcccgac gtaaccttga gcgattggga atcgaccttt gaacgacgcg gtcaggaagg
      241 gcagcgaatt tcatcgagcg agacctgttt gcatcgtcag cagggcgtat tatccgcgca
      301 gcaattacgc gatctgtcgt tgcggcgctt cacggtggcc cggacgattc ggcgagcggc
      361 aaagcggctg accgcacgcg tattccaatc atcgaacacc tatggtgcac gcggtcccgc
      421 agtgcgcatc ggcatgcact tgatcaaagg tctttcacag gtggctgccg aacgcatcat
      481 ggcggctcgc agcgaggcgc agtttaccga cgtcgacgat ctggcacgtc gcgcggcatt
      541 gacgcggcgt gatctcgaag cgctcgccgc ggcgaacgcg ctggtcagca tcgcaggcca
      601 tcggcgcgaa gcgtggtggg cagtgaccgc gcagcacaca gtgcccaagc tgctgcgcga
      661 tgcgccgatc gacgaagcgc ggttggcatt gccgcaggca gccgagagtc gcgagatcgt
      721 cgatgattac gcgagcctcg gcctcacgct caatcgtcat ccgctcgcac tcttgcgcga
      781 gcgtcttgcc aaacaacgtt ttcgcacggc agccgaactc gccgcgtgtc agcacggcac
      841 gctggcgcgc gcttgcggca tcgtcaccgt gcggcagcgg ccgggcacgg caaacggcac
      901 ggtgttcgtt tccctcgaag acgagaccgg ttcgatcaat gtgatcgtct ggccattgct
      961 ggtggaaaag cagcgcaagg tgctactggg ttcatcgttg cccgcggtgt atggcgtctt
     1021 gcagcgtgac gacggtgttt ctaccggtgg tcacggcgcc ggcaacgtga tgaacgccaa
     1081 tcaagcgggc agggcgaaaa agaaacagaa gggcgaggtc attcatctcg ttgcgcatcg
     1141 actcgaggac cacagcgaat ggctcggcga tctggctacg gcgagccgcg attttcattg
     1201 atgccgatct gcagtggtca gtcttgcaga cccatgccag gcaggcgtgt cgtcgggcag
     1261 tgaaacgtgg gcggcacctg ccgcggttaa gttcgcagcc gagtaccagg tcagcgcttc
     1321 ggcttgctac ggaaatgcgg gcggcacctg ccgcggttaa gctcgcagcc gagtgccagc
     1381 tcagcgcttc ggcttgctac ggaaatgcgg gcagcccttg ccgcggttaa gctcgcagcc
     1441 gggcgctacg ccggcatttc accttgccac ggaaacgcgg gcagcactgc cgcggttaag
     1501 ctcgcagccg cgcgctaggc cagcgtttca cctcgccacg gaaacccggc cagtacttgg
     1561 cggatcgcca cgtcgaacgg cgtgcggcgg ccgatgccga gcgcctcgag ccggtccgac
     1621 gccaggtggc agttatgcgg acgcggcgtc gcgccggtgg gcatgtgctg tggcgtcagt
     1681 tgcgcatcga gttgcagcgc ttgcgcaagc cgcatggcga tttcatattt attcatcggc
     1741 tcgtcgcccg accattgcac gatgccgtgg atcgcttcgc cctgtgcatg ccgttcgagc
     1801 atctgccgga tcacgaacgc gacgtccggc gtaaaggtgg gataacggat cgcccatgca
     1861 tccatgaccg ccgccttgcc atcgcgcgaa gccgatgccg caatcgccgg cacgagactc
     1921 gtcaccgccg attctgccca atcgacgatc ggtccataca gcaacggcaa acgcagcacg
     1981 cagccgagat cggtggaatc cgtcagcgct cgctcgcctt cgagtttgct gcgcccatag
     2041 gcattgagcg gcgcgggcgc gcaatcgtgc cgatagggcg ggtgagtgcc gtcgaacaca
     2101 taatcagttg agatcgagag cgtccatgcg ccgcgccgcc gtgcggccgc agccagtgtg
     2161 cgtaccgcat cgacgtttaa cgcccgggcg agcgccgggt cgtgctcgca cacatccggc
     2221 cgacgttccg cggcagcaat cacgagcgcg tccggcgctt cgcgctcgac aaattgctcg
     2281 accgcctgcg cgtcgcgaat atccagccca acggtgttcg gccccggccg gctgaacgcc
     2341 gtcgcgagaa tttgccagcc agcctgtcgt gccagttcgt cgacgagaac ccggccgagc
     2401 agtccggaag caccaatgac ggcaactttg aacatgacgg tcgctagcct agcgggccga
     2461 gccgttggtc acggccgtaa ccatgtaacc ggcctcgtcg attgcggtct tcagcgtgtc
     2521 agccgattgc gccgattcga ccgccacgcg gccggatgtc agatcgacgt gtacctgtgc
     2581 ggctccatca tgttcacgga tcgcgtgtgt gactgccgcc acgcaatgct ggcagctcat
     2641 gccttctacc cggaattcga tcgtcacgga gattgcctca aaaaagtaaa tgctgcgatg
     2701 gttgaattat gcctgccgat gcgcacttga gtagctgacc ttcccactat gggaaggtgt
     2761 acgatcgacg cacatactgg ggaaacagtc atgaacatcg gtgaagcggc ccgcgtgtcg
     2821 ggcgtcacgg cgaaaatgat tcgctactac gaaagcgtgg gcctgctggc ggcaaaggcg
     2881 cgcacgagcg ccggctatcg cgtatacggg ccgcag
//
LOCUS       DATASH010001079        10581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_7111187,
            whole genome shotgun sequence.
ACCESSION   DATASH010001079 DATASH010000000
VERSION     DATASH010001079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 10581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10581
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7111187"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(303..1496)
                     /locus_tag="VHV99_28515"
     CDS             complement(303..1496)
                     /locus_tag="VHV99_28515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3639355.1"
                     /translation="MSTSDASTPASADRSLRGLLLLLATIAGVSVANIYYNQPLLDNF
                     RQSFPKSASWVGAVPAVTQLGYAAGMLLLAPLGDRFDRRFLILLQIGGLCVALVLAAA
                     APTLSVLIIASLAIGVLATIAQQAVPFAAELAPASERGHAVGTVMSGLLLGILLARTA
                     SGIIAEYFGWRAVFGASVVALLVLAAVIILRLPKSKPTSTLPYGKLMASMWHMVVEHR
                     ALREASLTGAAMFAAFSIFWSVLALLLAGAPFHLGPQAAGLFGIVGAAGAMAAPLAGK
                     FSDRRGPRAIITLSIVLVAISFVVFALSAKSIAGLVIGVIVLDIGVQAAQISNQSRIY
                     ALKPDARSRVNTVYMVAYFIGGAVGSGVGAVVWPAFGWVGVSVAGVVFAGLAGWNHLR
                     LPARG"
     gene            1999..2484
                     /locus_tag="VHV99_28520"
     CDS             1999..2484
                     /locus_tag="VHV99_28520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HEX3639356.1"
                     /translation="MNTTTQPAPRAIGGWLLLVLICLAAWVLSTAVALRDPLKLMLEW
                     ELLAVFVRPETHGWYRTVIAMVGCDVIAGSFIVAGAGWLALLAWRKSARFPVQVQAWL
                     LAIVVMRTIAYLLGDHMAHAIGIAIAIPLDGFIQAVVAAALGIPYFRRSRRVQQTFVV
                     A"
     gene            complement(2503..3330)
                     /locus_tag="VHV99_28525"
     CDS             complement(2503..3330)
                     /locus_tag="VHV99_28525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013338709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HEX3639357.1"
                     /translation="MLLAQISDLHIKRPGALAYRRVDTGAYLARTIAALNALEPRPDA
                     VIMTGDLVDQGHPEQYEHLKVLLAPLEIPYYLLVGNHDERAALRAAFPDRAELHNGGE
                     FVQYAVDVGPLRIIALDSLVPGESAGNLCDARLTWLAAQLDAAQGKPTVVALHHPPFV
                     CGIGHMDELRLDPAAAGKLAALLANYPNVERVICGHVHRPMFVRFGGTIASAVPAPAH
                     QVALDLHEDAPSAFVMEPPAYALHRYDPATGIVTHHAYVDAADGPYPFYEPEGELID"
     gene            complement(3375..4421)
                     /locus_tag="VHV99_28530"
     CDS             complement(3375..4421)
                     /locus_tag="VHV99_28530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3639358.1"
                     /translation="MKLDSVPITLTQCAKTFRGTRVLEPLDLHIGAGETLVLLGPSGC
                     GKTTTLRMIAGLETPDAGGAIAFGDEDVTALPIEKRQVGMVFQSYALFPNLTVRGNIG
                     YGLKIKRVPADTARQRVDELLAMMRLTAHADKPIDQLSGGQRQRVALARALAVQPRVL
                     LLDEPLTALDARLRDTLRSEMNTLLRELGITTVYVTHDQAEAMELGDRIVVMSAGRIE
                     QIGSPRDIYYRPANRTVAQFVGTINRLAGERRAGLLTTTGGTVPLPAGPAQASAAHEI
                     FFRPEDAYLADPADAQLRGHIESTAFLGERTRLTISGAAPDALLIDVAGRVELARGTP
                     VGISIAQDALIALS"
     gene            complement(4418..5326)
                     /locus_tag="VHV99_28535"
     CDS             complement(4418..5326)
                     /locus_tag="VHV99_28535"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3639359.1"
                     /translation="MNSITAPVQPSAKARSRRLRLPTSRTWLAAGQWLVTLLLCAFLI
                     VPVVMSIMAGLTVNYFKGVSSGLTLRWLGEVWTQYHDSVFLSLEVAAATLFITLLTGV
                     PAGYVLARSKTRLSRIIEEFLVLPIALPGLASALALLVVYGGFTMFRMSVAFIVVGHV
                     VFTLPFMVRAVAAVCASSDLRTLEEGAASLGASFFQRFITVVLPNARPGIVAGALAVL
                     TLSIGEFNLTWMLHTPDTKTLPVGLADTYASLRIEIGSAYTILFFIMTMPLLVAMQWL
                     GVDATGQRSAGKKRAKASPLKSTPTP"
     gene            complement(5365..6189)
                     /locus_tag="VHV99_28540"
     CDS             complement(5365..6189)
                     /locus_tag="VHV99_28540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019255790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3639360.1"
                     /translation="MNDVTFPLRWRIALIAPALAVFIAFWLLPMGVLAQISGDGHAFA
                     TYRAMLTNPRYMSSLGATVMLSAAVTATTLVLSVIAGLLLARREFPLKRTLLALLTFP
                     LAFPGVVVGFMVIMLAGRQGLIGALSLKLTGDRWVFAYSMSGLFLGYLYFSIPRVIVT
                     VMASATKLDASLEEAARSLGASPWRITRDIVLPALSPGLIAAGAVCFATAMGAFGTAF
                     TLATDIDVLPMTIYTEFTLNANMVTAAGLSIVLGIVTWLVLAFARSVSGSAVAASA"
     gene            complement(6186..7265)
                     /locus_tag="VHV99_28545"
     CDS             complement(6186..7265)
                     /locus_tag="VHV99_28545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018435308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3639361.1"
                     /translation="MIRSSTRLASSIKPLWRRLARTTGAALSAGMIVTATLAALAPQS
                     AHADETAICYNCPPEWADWASQIKAIQQKTGIRMPFDNKNSGQAIAQLMAEQKSPVAD
                     VVYLGVSSAFQAKDKGVIQPYKPAHWDDIPANMKDPQGYWFSIHSGTLGFFVNKDALE
                     GKPVPRSWADLLKPEYKGMIGYLDPSSAFVGYAGAVAVNQALGGTLDNFEPGLDWFRK
                     LKANAPIVPKQTAYARVMSGEIPILLDYDFDAYRAKYKDHANVEFVIPKEGTISVPYV
                     MSLVKGAPHEANGKKVLDFVLSDEGQKLWAEAYLRPVRANVMSPETAAKFLPASDYAR
                     AKPVDFGKMAEKQSSFGERYLQVMH"
     gene            complement(7427..8482)
                     /locus_tag="VHV99_28550"
     CDS             complement(7427..8482)
                     /locus_tag="VHV99_28550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006052466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX3639362.1"
                     /translation="MIPTIKDVAAHAGVSIATVSRAINAPHTVNPLTLDKVRQSIDAL
                     NFRPSPLGRQLRGERTRLIGVILPTLANPVFAECLQGIDELASAQGYRLMLMTTQYDA
                     DRERHAIETLREHRVEGLILTVADADTHPLLDELDRAGLLYELMHNDTVRRPSVSVDN
                     RLAAFDGVRMLIAHGHRRILMLAGTLAASDRARQRHLGYAQAMQQAGLTPAPALEVDF
                     NADELSPSVLAHLTSGANRPTALFCSNDLLAMVVMRGLRRARLQIPHDMSILGFDGLA
                     MGELLSPPLASICAPNREIGCAAWQRLLTRVTGTCETSDETSLTLTLPHSLREGATIA
                     AISNRSETSAATATRFA"
     gene            8666..8750
                     /locus_tag="VHV99_28555"
     tRNA            8666..8750
                     /locus_tag="VHV99_28555"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8700..8702,aa:Leu,seq:taa)
     gene            complement(9327..9782)
                     /locus_tag="VHV99_28560"
     CDS             complement(9327..9782)
                     /locus_tag="VHV99_28560"
                     /inference="COORDINATES: protein motif:HMM:NF025292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HEX3639363.1"
                     /translation="MEAIGQERQNCTGPQLGTWKLRSFTTEDLATGQKTDLFGAHPSG
                     YLSYGSDCRVSGILVKEGRKAPNSLVPTDAERIELYNGLIAYAGTYSIEGDKVSHRID
                     ASWNQSWTGTTLMRQFRIDGKSLYIRTLPAKNPLTGKESSAELVFDKVE"
     gene            complement(10159..>10581)
                     /locus_tag="VHV99_28565"
     CDS             complement(10159..>10581)
                     /locus_tag="VHV99_28565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX3639364.1"
                     /translation="VCSEALRLRPVAAIQTFEPLIDVCLGGVAVPAGTRMLFLTRPSM
                     LDPRNFVRPETFDPDRWRHPRDPSEGVHDQKAYLTFGAGPRVCPGRYLAAVEMRLVIS
                     MLMRHFKIELAIDPAAIEEISAFTMVPNTMPVRLKPRA"
BASE COUNT         2169 a         3233 c         3296 g         1883 t
ORIGIN      
        1 caatatcaat tcaaggaaag gccaacgccg taggcataca gacaaacaag cgcaacgcag
       61 acaaagaaat caaggaaagg cccacgccgc aggcatacag acaaacaagc gcaacgcagg
      121 caaacagctc aaggaaaggc ccacgccgca ggcacacaga caaacaagcg caacgcaggc
      181 aaacagctca aagaaaggcc cacgccgtag gcatacagac aaacaagcgc aacgcaggaa
      241 aacagcccaa gaaaaggccc acgccgcagg cacacagaca ataagcgccg caggcaaaaa
      301 ccttaacccc tggctggaag cctcaaatga ttccaccccg ccagcccagc aaaaacaacc
      361 ccagcgacgc taactccaac ccatccaaaa gccggccaaa ccaccgcacc gacccccgac
      421 cccacggcac cgccaataaa ataagccacc atataaacgg tattcacgcg actccgcgca
      481 tcaggcttca acgcataaat ccgcgattga ttcgaaatct gcgcagcctg caccccaata
      541 tcgaggacga tcacgccaat caccaatcca gcaatgctct tcgccgacaa ggcaaaaacc
      601 acaaacgaaa tcgccaccag cacaatcgac agcgtgataa tcgcccgcgg tccgcgccgg
      661 tcggaaaact tgccggcgag cggcgcggcc atcgcaccgg cggcacccac aatcccgaat
      721 agcccggccg cctgcggccc aagatgaaac ggcgcgcccg ccagcaataa cgccagcacc
      781 gaccagaaaa tgctgaacgc tgcgaacatc gctgcgcccg tcaacgaggc ttcacgcagc
      841 gcgcgatgtt cgaccaccat gtgccacatc gaggccatca gtttgccgta cggcagcgtc
      901 gacgtcggct tactcttcgg cagacgcaga atgatcaccg ccgccaacac cagcagtgcg
      961 acgaccgagg caccgaatac cgcgcgccag ccgaaatact cggcgatgat gcccgaggca
     1021 gtccgtgcca gcaaaatccc cagcaacagt ccactcatca cggtgccgac cgcatggcca
     1081 cgttcggatg cgggcgcgag ttcggccgcg aacggcaccg cctgttgcgc gatggtggca
     1141 agcacgccga tcgcaaggct cgcgatgatc agcaccgaca acgtcggcgc cgcggccgcc
     1201 agcaccagcg cgacgcacaa gcccccgatc tgcaacagga tcaggaagcg tcgatcgaaa
     1261 cggtcgccga gcggcgccaa caacaacatc ccggcggcgt agccgagctg cgtcacggcc
     1321 ggtacggcgc cgacccacga cgcgctcttc ggaaacgact gccggaaatt gtcgagcaac
     1381 ggctggttgt aatagatgtt tgcgaccgac acgccggcaa tcgtggcgag cagaagcagc
     1441 aagccgcgca acgaccggtc ggcggaagcg ggagtagaag cgtcagatgt ggacatgaaa
     1501 gagggaaatc atcgacggac cggcccatgc acggtcctca agcgtgccga tcataccgga
     1561 gcaggaggga tcgtgctgac ggtcgggagc gtctggttcg atgcgtcaaa gacgttaagg
     1621 cagagacgag ggcgaaatga ctgcaaagca ggttcgaggc gaaggccgtt tcgtttcgaa
     1681 tggccccgcg gtaaaaatga gatggtcgtt cggttttcgt tcgtagaatc ggcacgcgcc
     1741 gcaatgggtg caattcaaac tatgacgtga cgactccatg aatataacga cgaaacccga
     1801 gccgaaaggc ataggtggct ggctgctgtt gccgctgggc ggcctggctc tcagccttgc
     1861 gtgggatatg gctgatctga tttcactgat aacacgggca ctggccacac gtcgctccgg
     1921 ttgcgacggc ggcacacggt cccggcgcgt aaaaaatacg ttcacccaag cttgatcgta
     1981 gtgagcggac ataaaaagat gaacacaacg acacaacccg caccgcgggc catcggcggc
     2041 tggctgttgc tggtcctgat ctgcctcgcc gcatgggtgc tttcgaccgc agtcgcattg
     2101 cgcgacccgc tgaagctcat gctcgaatgg gaactgctgg cggtattcgt gcgtcccgaa
     2161 acccacggct ggtatcgcac cgtgatcgca atggtcggct gcgacgtgat cgccgggtcg
     2221 ttcatcgttg ccggcgccgg ttggctggcg ctgctcgcgt ggcgcaaatc ggcgcgcttt
     2281 ccggttcagg tgcaggcgtg gttgctggcc atcgtcgtca tgcgaaccat cgcctatctg
     2341 ctgggcgatc atatggccca cgccatcggc atcgccatcg cgattccgtt ggacggtttc
     2401 attcaggctg ttgtcgccgc cgcgctcggg ataccgtatt tcaggcgttc ccggcgcgta
     2461 cagcaaactt ttgtcgttgc ctgaaccggc gacgcgacgg cttcaatcga tcaactcgcc
     2521 ttcaggttcg taaaacggat acggcccatc cgctgcatcg acatacgcat gatgcgtgac
     2581 gatgcccgtc gccggatcgt agcgatgcag cgcgtaagcg ggcggctcca tgacgaaagc
     2641 ggagggcgca tcctcatgca gatcgagtgc gacctgatgc gcgggcgccg gcaccgccga
     2701 cgcaatcgtg ccgccaaaac gcacgaacat gggccgatgc acatggccgc aaatcacccg
     2761 ctccacattc ggatagttcg cgagcaaggc ggcgagtttg ccggcggccg cgggatcgag
     2821 ccgtaattca tccatatgcc cgatgccgca gacgaacggc ggatgatgca acgccaccac
     2881 cgtgggcttg ccttgcgcag catcgagctg tgcagcgagc cacgtcaagc gtgcatcgca
     2941 cagattgccg gcgctttcgc ccggcaccag cgaatcgagc gcgatgatcc gcaatgggcc
     3001 gacgtccacc gcgtattgca cgaactcgcc gccattgtgc aattccgcgc gatcgggaaa
     3061 tgccgcgcgc aacgcagcgc gttcgtcgtg attgcccacc agcaggtagt aggggatctc
     3121 gagtggcgcg agcagcactt tcaaatgttc gtactgttcc gggtggccct gatcgacaag
     3181 gtcgccggtc atgatcacag catcgggacg cggttccagc gcattgagcg cggcgatggt
     3241 gcgcgcgaga taagcaccgg tgtcgacgcg gcggtaagcc agcgcgccgg gccgtttgat
     3301 atgtaggtcg ctaatttgag ccagcagcat ggggaagtcc tttcttgtgg cgcgctaaaa
     3361 cacgcgcggc gttattacga tagcgcaatc agcgcgtcct gggcgatcga tatcccgacc
     3421 ggcgtgccgc gcgcgagttc gacccggccg gcgacgtcga tcagcaaggc gtccggcgcg
     3481 gcgccactaa tcgtcaagcg ggtgcgctcg ccgagaaacg ccgtgctttc gatatggccg
     3541 cgcaattggg cgtcggccgg gtcggcaagg tatgcgtctt cagggcgaaa gaagatttcg
     3601 tgcgccgcgc ttgcttgcgc gggaccagcc ggcaggggga cagtgccgcc ggtcgtggtc
     3661 agcagacccg cgcgccgttc gcccgccagc cggttgatgg tgccgacgaa ctgcgcgacg
     3721 gtacgattcg ccggacgata gtagatgtcg cgcggcgagc cgatctgctc gatacgcccc
     3781 gcgctcatta cgacgatgcg gtcgccgagt tccattgctt cggcctgatc gtgcgtgacg
     3841 tagacggtgg tgatgcccag ttcccgcagc agcgtattca tttcgctgcg caacgtatcg
     3901 cgcaaccgcg cgtccagcgc ggtgagcggt tcgtcgagca gcagcacgcg aggttgcacg
     3961 gcaagtgcgc gcgccaatgc aacacgctga cgctgcccgc ccgacaactg atcgatgggt
     4021 ttgtccgcgt gggcggtgag gcgcatcatc gcgagcagtt cgtcgacgcg ttgccgcgcg
     4081 gtgtcagcgg gtacgcgttt gatcttcagt ccataaccga tgttgccgcg cacggtcaga
     4141 ttcggaaaca acgcgtagct ttggaacacc atgcccacct ggcgtttttc gatcggcaac
     4201 gcggtgacgt cttcatcgcc aaatgcaatg gcgccgccgg cgtcgggtgt ctcgaggccg
     4261 gcgatcatgc gcagcgtggt ggtcttgccg caaccggacg gcccgagcag caccaatgtc
     4321 tcaccggcac cgatgtgcaa atcgagcggt tcgagcacgc gcgtgccgcg aaacgttttc
     4381 gcgcattgcg tgagcgtgat gggaacggag tcgagtttca tggcgtaggt gtggacttca
     4441 aaggagaggc cttcgcgcgc tttttacccg cgctgcgctg gccggtcgca tcgacgccga
     4501 gccattgcat tgcaacgagc agcggcatgg tcatgatgaa aaacagaatc gtgtacgcgc
     4561 tgccgatttc gatgcgcaac gacgcatagg tgtcggcgag accgaccggg agcgtcttgg
     4621 tgtcgggcgt gtgcagcatc cacgtcagat tgaattcgcc gattgagagc gtcagcaccg
     4681 ccagcgcgcc ggcgacaatc ccgggccgcg cattcggcag cacgaccgtt atgaagcgct
     4741 gaaaaaagct cgcgccgagg cttgccgcgc cttcttcgag cgtgcgcaga tcgctgctcg
     4801 cgcacacggc agcgaccgcg cgcaccatga acggcagcgt gaacaccacg tgcccgacga
     4861 cgataaacgc gacgctcatg cgaaacatcg tgaagccgcc gtacaccacc agcagggcaa
     4921 gagcggaagc gaggccaggc agggcgatcg gcagcaccag aaactcttcg atgatgcgcg
     4981 agagccgcgt cttgctgcga gccagcacgt agccggccgg cacaccggtc agcagcgtaa
     5041 tgaacaaggt tgctgctgcc acctcgagcg acagaaacac ggagtcgtga tactgcgtcc
     5101 acacttcgcc gagccagcgc agcgtgagcc cactcgatac gcccttgaag tagttgaccg
     5161 tgagccccgc catgatcgac atcacgaccg gcacgatcag aaacgcacac agcagcagcg
     5221 tgacgagcca ctggccggct gccagccacg tgcgtgaggt tggcagcctg aggcgccgcg
     5281 agcgtgcttt tgcgctcggc tggactggag cggtgatgga attcatcgaa gtcttgtcat
     5341 tcggtttgga catgcggctc atgctcatgc gctggccgcg accgccgagc cgctcacgct
     5401 gcgagcgaac gccagcacga gccatgtgac gatgccgagc acgatcgaga gacccgcggc
     5461 cgtcaccatg ttcgcgttca gcgtgaactc ggtgtagatg gtcatcggca acacgtcgat
     5521 gtcggtggcc agtgtgaagg ccgtgccgaa cgcgcccatc gcggtcgcga aacagacggc
     5581 gcccgccgcg atcagacctg gcgacaacgc gggcaatacg atgtcgcgcg taatgcgcca
     5641 cggcgatgcg ccgagcgaac gcgcggcttc ttcgagcgat gcatcgagct tggtggcgga
     5701 ggccatcacc gtgacgatca cacgcggaat cgagaagtaa aggtagccga gaaagaggcc
     5761 gctcatcgaa taagcgaaca cccagcggtc tccggtgagt ttcagcgaga gcgcgccgat
     5821 caaaccctgg cgtcccgcca gcatgatgac catgaagccg accacgacgc ctgggaatgc
     5881 caatggaaac gtcagcagcg ccagcaacgt gcgcttgagc gggaactcac ggcgcgccag
     5941 cagtaagccg gcgatcaccg agagcaccag cgtcgtcgcc gtaaccgccg cggacaacat
     6001 cacggtcgcg ccgaggctcg acatgtagcg cggattggtc agcatcgcgc gataggtcgc
     6061 gaaggcgtgg ccgtcgccgc tgatttgcgc gagcacaccc atcggcagca gccagaacgc
     6121 gatgaaaacc gccagcgccg gcgcgatcaa cgcgatgcgc cagcgcagcg ggaacgtgac
     6181 gtcgttcaat gcatcacctg gagataacgc tcgccgaagc tcgactgctt ctcggccatt
     6241 ttgccgaagt cgaccggttt ggcacgggca tagtcgcttg cgggcaggaa tttagcggcg
     6301 gtttccgggc tcattacatt cgcgcgtacc ggacgcagat aggcttcggc ccacagtttc
     6361 tgaccttcgt ccgagagcac gaaatcgagc accttcttgc cgttcgcttc atgcggcgcg
     6421 cccttcacga gactcatcac gtaaggcacc gagatcgtgc cttccttcgg aatcacgaat
     6481 tcgacattgg cgtgatcctt gtacttcgcg cggtaagcgt cgaagtcgta gtcgagcaga
     6541 atcgggattt cgccggacat cacgcgcgcg taggcggttt gcttcggcac gatcggcgcg
     6601 ttggccttga gcttgcggaa ccagtcgagg cccggctcga aattatcgag cgtgccgcca
     6661 agtgcctgat tcaccgcgac cgcgcccgca tagccgacaa acgcgctcga cggatcgagg
     6721 taaccgatca tgcctttgta ttcaggctta agaaggtcgg cccatgaacg cggaaccggc
     6781 ttgccttcga gcgcgtcctt gttgacgaaa aagcccagcg tgcccgagtg gatcgagaac
     6841 cagtagcctt gcgggtcttt catgttcgcg gggatgtcgt cccagtgcgc gggcttgtac
     6901 ggctggatga cgcctttgtc tttcgcctga aacgccgacg acacgccgag ataaaccacg
     6961 tcggcgaccg ggctcttctg ctcagccatc agttgtgcaa tcgcctggcc ggagttcttg
     7021 ttgtcgaacg gcatgcggat gccggtcttt tgctggatcg ccttgatctg gctcgcccag
     7081 tcggcccatt cgggtgggca gttgtagcag atcgcggttt cgtcggcatg ggcggactgc
     7141 ggcgcgagcg ctgcgagcgt ggcggtgacg atcatgccgg ccgacagcgc ggcgcctgtg
     7201 gtgcgggcga ggcggcgcca aagcggtttg atcgaggaag caagacgggt tgaagagcgg
     7261 atcactgcgg gtctcctgaa gaggaagaga gcgggtggtt cgggattcga agtagatgac
     7321 gcaaacaggg tgttgccgaa gggtgtttcc aaacgatcag cgcaaaccgt tcaaaaaaac
     7381 agcgtgggcg agcggaccgg agcatatgca cgtcttacgc aagcgactac gcaaaccgcg
     7441 tcgcggtagc cgccgaagtt tcgctgcggt tcgaaatcgc cgcgatggtg gcgccttcgc
     7501 gcaagctgtg cggcaacgtc agcgtgagcg acgtttcgtc tgatgtttcg caggtgccgg
     7561 tgacgcgcgt cagcaaacgt tgccaggcgg cgcagccgat ctcgcgattg ggcgcgcaga
     7621 tacttgccag cggcggcgac agcaactcgc ccatggcgag tccgtcgaag ccgagaatcg
     7681 acatgtcgtg cggaatctgc agacgcgcac ggcgcaggcc acgcatcacg accatggcga
     7741 gcagatcgtt actgcagaag agggcggtcg ggcggttcgc gccgctggtc agatgagcga
     7801 gcacggacgg cgacagttcg tcggcattga aatcgacctc gagtgcgggc gcgggggtga
     7861 ggccggcctg ctgcatcgcc tgcgcgtagc cgaggtgccg ctgacgggcg cggtccgagg
     7921 cggccagtgt gccggcgagc atcaggatgc ggcgatggcc gtgcgcgatc agcatgcgta
     7981 cgccgtcgaa cgcggcgagg cggttgtcga ccgagaccga tgggcgtcgc acggtgtcgt
     8041 tatgcatgag ctcgtagagc aggccggcgc ggtcgagctc gtcgagcagc ggatgcgtgt
     8101 cggcatcggc gacggtcagg atcaggcctt ccacgcgatg ctcgcgcagc gtttcaatgg
     8161 cgtggcgttc gcggtcggca tcgtactggg tggtcatcag catgagccgg tagccttgcg
     8221 ccgaggcgag ctcgtcgatg ccttgcaggc attcggcgaa cacggggttg gcgagcgtcg
     8281 gcaggatcac gccgatgagg cgcgtgcgtt cgccgcgcaa ctgccggccc agcgggctgg
     8341 ggcggaaatt gagggcgtcg atggattgac gcaccttgtc gagcgtgagc gggttgacgg
     8401 tgtgcggcgc gttgatcgcg cgcgagaccg tggcgatgga aacacccgcg tgggcagcga
     8461 cgtctttgat cgttgggatc atcgtttccg ctctggttgt aaacgttttc gtgagcggat
     8521 tatcgggaag gtttgtgact cccggatgac ttccgtattg acgcctgcgc ggtgtgaggc
     8581 ggtatggtac gcggctggat cgagccagtt tggtgcgccc gccgggcaat ggtagaattc
     8641 gcgtccacaa gcggttctgg cccctgccgg ggtgatgaaa ttggtaaaca tagcggactt
     8701 aaaatccgcc gcttcacagc ttgccggttc gagtccggtc cccggcacca aggcgactcc
     8761 tcacttattc tctcctttcc ccgctttctc ccgtctggta tggtttgggc cgggttttgg
     8821 ctgagttcgc gtctctacgt tttaccccct tttcccgcta ttttctggta gtggttcccc
     8881 ggaatttacc caatctttcc ccagggggaa tttaaatggc gtcgatatac aagacagagg
     8941 gcggcacgtg gcgggtgcag gtcgccaggc gcggcatcag attgagcggt acgttccggg
     9001 cgtcggccgc cgcctaactg tgggcagcgt cgaaagcctt gacttgttcg gcggtgagcc
     9061 gtgcctagct caatgagttc tcgcaagcat tcgccgcgcg ccggcgtcga gcagcgcact
     9121 ggcagtgctc acgcgttgcg ggttcaaagg acgcagttcg aaccgttgca gtcctttgct
     9181 agcgccgaat gcagtcgttg ccaacctgcg agagcagacc ctaatttgaa acaatcgtgc
     9241 taacaggtgc agggcaaacg ggcacagcat ggatgcccct cgtccgtgtt gagcgcgagt
     9301 ttagcccgca ggggctggac gcacgcttac tcgaccttat cgaagaccaa ctcggctgag
     9361 ctttccttgc cggttagtgg gttcttggca ggcagcgtcc tgatgtacaa ggacttaccg
     9421 tcgattctga actgacgcat caaagtcgtt cctgtccaag actgattcca cgatgcatca
     9481 atacggtgac tgaccttgtc gccctcgata ctgtaagtcc ccgcgtatgc gataagccca
     9541 ttgtagagtt cgatgcgctc cgcgtcggtc ggcaccaaac tgttaggcgc ctttcgccct
     9601 tccttgacca aaatgccgga cacccggcag tcggaaccat agctgagata gccgcttggg
     9661 tgcgcgccga acaaatcggt cttttgtccg gtcgccaggt cttcggtcgt gaaggaccgt
     9721 agcttccacg ttcctagctg cgggccggtg caattctgcc gctcttgtcc aatagcttcc
     9781 aagcttccaa ggcaaagtac gcaacacact aagacagcag ggcgtttcat cttattcccc
     9841 tctgagtaag tcgacgcgtc acaactggcg tgccgtatgg ccgaaatcca attcgagcta
     9901 ttagacgtcg ctcattgctg tctcccggat tcgactttgc gtcttccgat tctgcggacg
     9961 ctacccgcaa tcatggccgc actcagacgc atggcgactg tctttggtcg accccttgcg
    10021 gccgttcaac ccaacacggc ccaaacggcc gcttccagga tacaacggtc attcttctcc
    10081 gttgtggcca tcccgcggtg ttgatggcga tcatgcttgg aacgtcgcaa tgagcgtggc
    10141 gcccagcgcg tcggcaattc aggcacgggg cttaagtctc acaggcattg tgttcggcac
    10201 catcgtgaag gcagaaatct cttcgatcgc ggccggatcg attgccagtt cgatcttgaa
    10261 gtgcctcatc aacatcgata taaccagcct catctcgacc gctgcaaggt aacgacccgg
    10321 acacactctc ggccccgcac cgaatgtcag gtacgccttc tggtcatgca caccttccga
    10381 cgggtcccgc ggatgcctcc aacggtcagg gtcaaacgtt tctggacgca caaaattccg
    10441 cgggtccagc atgcttggac gcgtgagaaa tagcatgcgt gttcctgccg gtacggcaac
    10501 accacccagg cacacatcaa tcaatggctc gaacgtttgt atggcggcaa ccggccgcaa
    10561 ccggagtgcc tcactgcata c
//
LOCUS       DATASH010001080         6151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4341702,
            whole genome shotgun sequence.
ACCESSION   DATASH010001080 DATASH010000000
VERSION     DATASH010001080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6151
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4341702"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..175
                     /locus_tag="VHV99_28570"
     CDS             44..175
                     /locus_tag="VHV99_28570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639365.1"
                     /translation="MCALRQYLHRVEQVLLDVGTRSLPRRHPTAQYDTGIDAPTVQG"
     gene            187..432
                     /locus_tag="VHV99_28575"
     CDS             187..432
                     /locus_tag="VHV99_28575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6723 family protein"
                     /protein_id="HEX3639366.1"
                     /translation="MARHKADVADDDFEIYASYHGTGDGRYVGGLKVVRKADRRILFP
                     FDGAPQIGPYATADEARRAAIDYGRKIVAADRAAPEK"
     gene            complement(579..815)
                     /locus_tag="VHV99_28580"
     CDS             complement(579..815)
                     /locus_tag="VHV99_28580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639367.1"
                     /translation="MTITTIRVSDTEVQVERTLYTFSQKTDADAFQRCLADTAIDSCY
                     RNHPPVSVRPTVPDEHPDDPSRGSTISPSLGGMP"
     gene            971..1231
                     /locus_tag="VHV99_28585"
     CDS             971..1231
                     /locus_tag="VHV99_28585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639368.1"
                     /translation="MQDTTPEGPNAEREAADPKARSKPSGTHYVEPSPLGIEPVEQTE
                     VAAQTNPQRSSERPKQTAEVPLGTGTHAEPPGGGGRESQEKK"
     gene            1337..1507
                     /locus_tag="VHV99_28590"
     CDS             1337..1507
                     /locus_tag="VHV99_28590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639369.1"
                     /translation="MPQDNQPLNPGDEASPDAPGVGEDTCQACHGTGKVEGAQCAICG
                     GTGKVLQGIGGG"
     gene            1795..2340
                     /locus_tag="VHV99_28595"
     CDS             1795..2340
                     /locus_tag="VHV99_28595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX3639370.1"
                     /translation="MYNDSKHGFDAAQTGYEAFMLEPHGWVPNNRTLPVILYHRALAP
                     DSGDLAAAFEILFEGNDWPPQWRDGIFDYHHFHATAHEVLGVTDGSAEVIVGGPGGRV
                     VKISAGDALLLPAGTGHCLQSFARHFQVVAGYPAGQQWDIRREALTPEEQATMEALPF
                     PAMDPIDGKRGPLIEHWLHAA"
     gene            2645..4729
                     /gene="tkt"
                     /locus_tag="VHV99_28600"
     CDS             2645..4729
                     /gene="tkt"
                     /locus_tag="VHV99_28600"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX3639371.1"
                     /translation="MQNDPALDQLCINTIRTLSMDAVQKANSGHPGTPMALAPVAYHL
                     WQNHLRYDPDEPLWPNRDRFVLSVGHASMLLYSLLHLANVKAVDDEGKPTGSPAVSLD
                     DIEHFRQIDSNTPGHPEYRMTTGVETTTGPLGQGLGNSVGMAMAARWYESHFNKPDAP
                     LFDYRVYALCGDGDMMEGISHEAASLAGHLKLSNLIWIYDSNRVTIEGHTDLAYSDEV
                     ESRFRGYNWHTLHVNDANDAAALEAAFVEAKSITDRPTLIVVHSIIGWGAPHKQDTSA
                     AHGEALGVEEVALTKKFYGWPEDKFFYVPDGVHERFAQGIGARGKTAREEWQAKYDAY
                     LKQYPELSREFAQMEAHELPAGWDSDIPTFDADPKGVATRESSGKVLNAIAARVPWMI
                     GGAADLSPSTKTNLKFEGAGSFEHDNYGGVNLHFGIREHGMGAAVNGLALSNLRPFGS
                     TFLIFSDYMKPPIRLSAIMEVPAIYVFTHDSIGVGEDGPTHQPIEQLASLRGVPGLTV
                     LRPGDANEVAQAWRVAMSHPRRPSCIVVSRQALPTLDRTRYASAEGVQKGGYVLADAP
                     DGQKPEVILLATGSELSLVVDAYEKLKSEGIAARVVSMPSWDLFERQDEAYQDSVLPP
                     DVDARVAVEQAASLGWDRYVGRLGAQVVMHTFGASAPLSDLKKKFGFTPERVYDAAKQ
                     QIARVKGKRSQA"
     gene            complement(4859..5041)
                     /locus_tag="VHV99_28605"
     CDS             complement(4859..5041)
                     /locus_tag="VHV99_28605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639372.1"
                     /translation="MLDKAQVLNALAARDIVPDATQRDAIAALVALPSNYAPDTLLSD
                     PEFHERVLPTIEQHRW"
     gene            complement(5093..>6151)
                     /locus_tag="VHV99_28610"
     CDS             complement(5093..>6151)
                     /locus_tag="VHV99_28610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3639373.1"
                     /translation="LAHELRNPLAPMRNVLEILRLKEFTDPQLSWSRDVFDRQLQHMT
                     HLVDDLLEVSRITQGKLELRKQRLELAGAMQSAMESARPTVQASAHHLSVTLPREALY
                     LDADPTRLSQMILNLLNNAAKYTPPGGSISLAAEREGDQAVIVVRDSGIGIPREHLDS
                     VFDMFSQLAPALERSQGGLGIGLALVRGLAELHGGTVAASSSGPGSGSEFVIRLPLSK
                     ATPEQADSAPAEVPRSAGLRVAIVDDNADAADSLAMVLELEGHEVRTAGDGIAGLELV
                     GEFAPHVVILDIGLPRLNGYEVARRIRLDHRDAGMLLIAVTGWGQQQDKQAAEEAGFD
                     HHFTKPVDPRELQRVLSR"
BASE COUNT         1200 a         1916 c         1997 g         1038 t
ORIGIN      
        1 cacctgctta ttcgtgcgtt gagcggtttg gcctgcgcgc cgcgtgtgcg cgcttcgcca
       61 gtacctgcat cgtgtggaac aggtattgct cgacgtcggc acgcgctcgc tgccgcgacg
      121 gcatccgacg gcgcaatacg acaccggaat tgacgcaccc acggtgcagg gataagggaa
      181 ataaccatgg cgcgacataa ggccgatgtt gctgacgacg acttcgagat ttacgccagt
      241 tatcacggaa caggcgacgg ccgttacgta ggtggactga aggtcgtcag aaaagctgac
      301 agacggattc tgtttccatt cgacggtgcg ccgcagatcg gcccctacgc gaccgccgac
      361 gaggcgcgcc gcgccgcgat cgattacggt cggaagatcg tggccgccga ccgcgcggcc
      421 ccggagaagt aaccggggca ggagcgctgg gccctgtgcg gcgctgtatt taaagcctgg
      481 cgataaagcc cggcgatgta tttaaaccca tcaaagcagt tggcccgccg ttggcgcgaa
      541 atcccaacca cagtgaaacg acgatctcac gcacggcctc aaggcatccc gccgagcgaa
      601 ggcgaaatgg tgctgccgcg gctcggatcg tccggatgct cgtccggcac cgtgggacgc
      661 accgacaccg gaggatggtt ccgatagcag ctgtcgatcg ccgtatcggc caggcagcgc
      721 tggaaagcat cggcgtcggt cttctgcgag aaggtgtaca aagtccgctc aacttgcact
      781 tcggtatccg aaacgcgaat cgtcgtgatc gtcatgacgc tctccttttt acaaaaaatg
      841 gtccaggcct tcagcatggg tgccgcaacg cccgtgccca cgggcgcctg ccgaatgggc
      901 aaggcggttg tccagagagg gaccggttgt tgctgcaccc catgatgtag ccgacttcaa
      961 ggagcgcacc atgcaggaca ccacacccga aggccccaac gcagaacgcg aagcagcgga
     1021 cccgaaagca cgcagcaaac cgagcggcac ccactatgtc gagcccagtc cgcttggcat
     1081 cgagccggta gaacaaaccg aagtcgccgc acagaccaat ccgcaacggt cgagcgagcg
     1141 tccaaagcaa accgccgagg tcccgctcgg caccggtaca catgccgagc cgccgggcgg
     1201 cggcggtcgc gaatcgcaag agaagaagtg acgtggagcg caatccgcgc ggatgatcgg
     1261 ccacgcggac ttaagcgatg tacgcaccgc gttgcattgc gcgcatcctg aagcaggaca
     1321 tccaacggag agacacatgc cacaggacaa ccagccgctg aatcccggcg acgaagcatc
     1381 gcccgatgca cccggcgtgg gcgaagacac ctgccaggcc tgccacggaa ccggcaaggt
     1441 ggaaggcgcc cagtgcgcga tttgcggagg cacggggaag gtgctgcaag gcattggcgg
     1501 cggatgattg aatcgcacgc actgatccgc gcgtggtgct cgctacggta atatcgtcat
     1561 atgtttggaa attttatccg cgaccgcggc gctgcgcgcc gtgcgtttca acagcacgcg
     1621 cgtaaaagag cgggcgcgtt ttatgcggcg gtgcacggat tcccgcattc ggcgcgtgag
     1681 gagcaggaac cgcccacaac gacatcacag gacgccagcg agacgcaacc gcagtcgccc
     1741 gcaacacgcg cggcacacga gccttacgcg cgccagcaag caaaggagtt gaccatgtac
     1801 aacgattcga agcatggatt cgacgcggct cagaccgggt acgaagcgtt catgctggag
     1861 ccgcacggct gggtgccgaa caaccgcacg ttgccggtca tcctctacca ccgcgcgctc
     1921 gcgccggaca gcggcgacct cgccgctgcc ttcgagattc tgttcgaagg caatgactgg
     1981 ccgccgcaat ggcgcgacgg gattttcgac taccaccatt ttcatgcgac cgcgcatgaa
     2041 gtactcggcg tgaccgacgg ttcagccgaa gtgatcgtcg gtggcccggg cggccgtgtg
     2101 gtgaaaatat ccgcgggcga cgccttgctg ctgcccgcgg gcacgggtca ttgcctgcaa
     2161 tcgttcgcgc gccacttcca ggtggtggcg ggctatccgg caggccagca gtgggatatc
     2221 cgccgcgaag cactgacgcc ggaggagcag gcgacgatgg aggcgttgcc attccccgcc
     2281 atggatccga tcgacggcaa gcgtggcccg ctgatcgaac actggctgca tgcggcctga
     2341 tcgcagcgca aggactggat tgcgccagct tttgagctca cgcaaacccg caagctgcct
     2401 gaaagaaccg tctccgatcc tcggtggaaa ccgattcact gcgcgatcca ttgcgcgatg
     2461 caatacgcga tgcaacgcgg cgcggatgta aagcgccgtg cagatttgcg cgcatcggcg
     2521 catcatcgat aggcctcgcg gtcttcatgt gtcgttccac gctcgtcaag acgaacaccg
     2581 gtcgccggcc acgcccatcg tgcgccccgg cgacatctcc aaccgccgaa caaggagtgt
     2641 ccccatgcaa aacgatcccg ccctcgatca gctgtgcatc aacacgattc gcacgctatc
     2701 gatggacgct gtgcaaaagg ccaattccgg tcaccccggc acgccgatgg cactagcacc
     2761 ggttgcgtat catctctggc aaaaccacct tcgctatgac ccggacgagc cgctgtggcc
     2821 caaccgcgac cgcttcgtgc tctcggtggg acacgcctcg atgctgttgt actcgctgct
     2881 gcatctggcg aacgtgaagg ccgtggacga cgagggcaag ccgaccggct cgcccgccgt
     2941 gtcgctggat gacatcgaac atttccgcca gatcgacagc aatacgccgg gacaccccga
     3001 gtaccgcatg accaccggtg tggagacgac caccgggccg ctcggccagg ggctgggcaa
     3061 tagcgtcggc atggcgatgg cggcacgctg gtacgaaagc cacttcaaca agcccgatgc
     3121 gccgctgttc gactatcgcg tctacgcgct atgcggcgac ggcgacatga tggaaggcat
     3181 ctcgcacgaa gcagcctcgc tcgcggggca tctgaagcta tcgaatctga tctggatcta
     3241 cgacagtaac cgcgtgacga ttgaaggcca cactgatctc gcctatagcg acgaggtgga
     3301 gagccgcttt cgcggttaca actggcacac gctgcatgtg aacgacgcga acgacgctgc
     3361 cgcgctcgaa gccgccttcg tcgaagcaaa aagcatcacc gacaggccga cgctgatcgt
     3421 ggtccacagc atcatcggct ggggcgcgcc gcataagcag gacacctcgg ccgcgcatgg
     3481 cgaggcgctc ggtgtcgaag aagtagcgct gaccaagaag ttctatggct ggcccgaaga
     3541 caagttcttc tatgtgccgg atggcgtgca tgaacgcttc gcgcaaggca tcggcgcgcg
     3601 cggcaagacg gcgcgcgagg aatggcaggc aaagtacgac gcttacctca agcaataccc
     3661 ggaactgtcg cgtgaattcg cgcagatgga agcacatgaa ctgccggcgg gctgggacag
     3721 cgacattccg accttcgatg ccgacccgaa gggcgtcgcc acgcgtgaat cgtcgggcaa
     3781 ggtgctcaac gcgattgccg cgcgcgtgcc atggatgatc ggcggggcgg ccgatctctc
     3841 gccgtccacc aaaacgaatc tgaaattcga aggcgcgggc agcttcgagc acgacaatta
     3901 tggcggggtc aacctgcact tcggcattcg cgaacacggc atgggcgccg cggtcaacgg
     3961 ccttgcgttg tcgaatctgc ggccattcgg ttcgacattc ctgattttca gcgactacat
     4021 gaaaccgccg atccgcctct cggcgatcat ggaagtgccg gccatctatg tgttcacgca
     4081 cgattcgatc ggcgtcggcg aagatggccc gacccatcag ccgatcgagc aactggcctc
     4141 gctgcgcggc gtgccgggct tgaccgtgct gcgcccgggc gacgcgaacg aagtcgcgca
     4201 agcatggcgc gtggcgatgt cgcatccgcg acggccgtcg tgcattgtcg tgtcgcggca
     4261 ggcgttgccg accctggacc gcacgcgcta tgcgtcggcc gagggcgtgc agaagggcgg
     4321 ttatgtgctc gccgatgcac ccgacggaca gaagcccgag gtgatcttgc tcgccacggg
     4381 cagtgaattg tcgctggtcg tcgacgcgta tgagaagctc aagagcgaag ggatcgcggc
     4441 acgggtggtg tcgatgccgt cgtgggatct tttcgagcgc caggacgagg cgtatcagga
     4501 ctccgtgctg ccacccgacg tggatgcgcg cgtcgcggtc gaacaggcgg cgtcgctcgg
     4561 gtgggaccgt tatgtcggac ggctcggcgc gcaggtggtg atgcatacgt tcggcgcgtc
     4621 cgcgccgctc tccgatctca agaagaagtt cggcttcacc ccggaacggg tgtatgacgc
     4681 ggcgaagcag cagattgcgc gagtgaaggg caagcgcagc caggcgtaga aataggcggc
     4741 tgcgcgatcg agacgttgtg ggtaatgaag aagccggcgg cgtgatgacc gccggctttt
     4801 tattcgcctc ttatagccgc gagttggcca ctgacgcgcg gctagttggc cggaatcgtc
     4861 accaccggtg ctgttcgatg gtcggcagga cacgctcgtg aaactcgggg tcggacagca
     4921 aggtgtccgg tgcgtagttc gagggtaacg cgaccagcgc cgcgatcgca tcgcgctgcg
     4981 ttgcatcggg aacgatatcg cgcgcggcga gggcgttgag tacctgagct ttatcgagca
     5041 tgaatcgaat ttaccgtccg ttagttggtt agtccgtcag tccgttagcc ggttagcggc
     5101 tcaacacgcg ttgcaattcc cgcggatcga ccggcttggt gaaatgatgg tcgaagccgg
     5161 cctcttcggc ggcctgtttg tcctgctgct ggccccagcc ggtgacggcg atcagcagca
     5221 tgcctgcgtc gcgatgatcg aggcgaatgc gccgcgccac ttcatatccg ttcaggcgcg
     5281 gcaagccgat gtcgagaatc accacgtgcg gcgcgaattc accgaccagt tcgaggcccg
     5341 cgatgccgtc gccggctgtg cgcacctcgt ggccttcgag ttcgagcacc atggcgaggc
     5401 tgtcggcggc gtccgcattg tcgtcgacga tcgccacgcg caagcccgcc gagcgcggca
     5461 cctcggccgg tgcactgtcg gcctgctccg gcgtcgcttt cgagagcggc agacggatca
     5521 cgaactcgct accgctgccc ggaccgctgc tcgacgcggc gaccgtgccg ccgtgcagtt
     5581 cggcgagacc gcgcaccagc gcgaggccaa tgccgaggcc gccttgtgag cgctccagcg
     5641 ccggggcgag ttgcgagaac atgtcgaaga cgctgtccag atgctcgcgc ggaatgccga
     5701 tgcccgaatc gcgcacgacg atcaccgctt gatcgccttc gcgttccgcg gcgagcgaaa
     5761 tgctgccgcc gggcggcgtg tacttggccg cgttgttcag caggttcagg atcatttgcg
     5821 agaggcgggt ggggtcggcg tcgagataaa gcgcctcgcg cggcaaggtc acgctcaggt
     5881 gatgcgccga cgcctgcacg gtgggccgcg ccgattccat ggcggactgc atcgcgcccg
     5941 cgagttcgag ccgctgtttg cgcaattcga gcttgccctg cgtgatgcgc gacacttcca
     6001 gcaggtcgtc gaccaggtgc gtcatgtgtt gcaattgccg gtcgaacacg tcgcgagacc
     6061 agctcagttg cgggtcggtg aattccttca gccgcaggat ttcgagcacg ttgcgcatcg
     6121 gcgcgagcgg attgcgcagc tcatgcgcga g
//
LOCUS       DATASH010001081         9454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3946024,
            whole genome shotgun sequence.
ACCESSION   DATASH010001081 DATASH010000000
VERSION     DATASH010001081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 9454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9454
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3946024"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1160)
                     /locus_tag="VHV99_28615"
     CDS             complement(<1..1160)
                     /locus_tag="VHV99_28615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HEX3639374.1"
                     /translation="MNALNEALPDYFQTARQLLFDQYQRRFPRIPTAQPVTIVEIDEE
                     TLATVGQWPWPRNRLADLIDAIAAQKPLAIGLDIYMPEPDQTSPDKVAANLPKSAAAL
                     AAGLRALPSHETILARSLRAAPTILGAAALDHAAFGSSTDLRSAPILVHGIDPIGSVR
                     RFDYVLASLPELQAAAHGQALLSVALEQGVVRHIPLVMGLSEKLVPNLPMEMLRLATG
                     SSALDVFADASGVRAVGVADVQVPTQPDGDIWLHFASIRSTLSRYVSARDVLQGKVDP
                     ERIRDKLVLVGLTGAGVTDMRTTALGELVPGIEIQAQVIETIVEGRFMRRPTWLKWAE
                     CAFIMTFGLLIIWYVPRPHSRFAVFIRAVPRGVAALGICLHLLNLLCCFYIFV"
     gene            complement(1348..1962)
                     /locus_tag="VHV99_28620"
     CDS             complement(1348..1962)
                     /locus_tag="VHV99_28620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX3639375.1"
                     /translation="MKAVIRSFGLAAAAACVMLVFLAGCSTPDKIILLPDPQGKVGAV
                     TVHSATGEQTIDKAYAGVDVTKGGTIEKTMDSQASVQTRYGELLAARPPRPMTFTIFF
                     LFDSATELAPESTATVQKLKAVLATWPAPQLVVVGHTDLAGSQEFNDRLSMQRAQTVA
                     AFLIKAGIPARQIETAARGKREPLVPTADGVPHRMNRRAVITIQ"
     gene            complement(1959..2378)
                     /locus_tag="VHV99_28625"
     CDS             complement(1959..2378)
                     /locus_tag="VHV99_28625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEX3639376.1"
                     /translation="MAAADARADDAAGVVKTLKGAVHIERAGASSGASIGSEVYGSDR
                     IVTGPESSVGITLRDTTQLSAGANTILDLNKFAFNTTTHDGVLDATVKRGSLAVISGK
                     LAKANPDAVRFSTPTTTLGVRGTEFIIEVGDHGESGR"
     gene            2653..2841
                     /locus_tag="VHV99_28630"
                     /pseudo
     CDS             2653..2841
                     /locus_tag="VHV99_28630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006478865.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(2849..4039)
                     /locus_tag="VHV99_28635"
     CDS             complement(2849..4039)
                     /locus_tag="VHV99_28635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007734751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX3639377.1"
                     /translation="MQRKLSHFVLFAMPYSSRLPLLYAVFALYAATAPCAASAQEPDP
                     SPERLSASGIHHAPHTDNGFENDEGPLARGSVWKWRWNRLIHHLPPPPESGYAFPVDH
                     PDVEWIKANRSENTMTWIGHSSALLQIDGVNILTDPVFSERASPFPFIGPKRRVPPGL
                     TLAELPHIDVVLISHSHYDHLDAASVQALNGQPGGPPLFLVPLGIKEWLAQKGITHAQ
                     ELDWGDRTQASELDFWFVPATHWSARTLTDRNETLWGGWVMKTPPRAAHPFSLYFAGD
                     TGYSGDAKRIGAAFGCFDLALIPIGAYAPRWFMAPQHVDPQQAVQIFQDVHAKKAIGI
                     HWGTFELADEPLDEPPKKLAAAAREAGLPDDAFTVLHHGQMIRLDNPREPVISPGTRD
                     SCAR"
     gene            4299..4700
                     /locus_tag="VHV99_28640"
     CDS             4299..4700
                     /locus_tag="VHV99_28640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639378.1"
                     /translation="MSTEWKRSFKLFIQRFWQPTSACMTCMPGSWGNVMSLAHWTIAF
                     HTGLLTGLLAVFLIFTPAAKLYTHRYGNALIVGCLTAIGDAYAHASHYGIPYVEHIVT
                     GAISALLTLAGSYLFEDRARRLRAVWARVFG"
     gene            5066..6355
                     /locus_tag="VHV99_28645"
     CDS             5066..6355
                     /locus_tag="VHV99_28645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007587648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3639379.1"
                     /translation="MTARQRWTILVASTGGALEVFDFVIFGFFARTIGSEFFPSQTGI
                     SAETLSFSVLAIGYLSRPLGGIFLGRLGDTHGRRLVFVSSAMIASISTLLIGILPSYA
                     VWGAAAPALLLVLRLIQGFCLGGELPGAVVYAVETVRGRPGFLCGIVFVAVNLALLLA
                     TSINLAVQWAFTPEQVNAFGWRIGFLFGGMVGLLSFALRRTLAETDEYARAIGARHRE
                     PLAVLFRNHFARLTTGAAACLLVGASGGIFIGFAPGYLQGLQYDAQQIASAQTRYLIV
                     VAVCILVTSYVGDLLPRRYVFRSGAVLSALFAPFFFMAAARHYVSLSVLFVTAGVVTS
                     LANGTFACALADMFPVDVRFSGVATAMNLGLATSLGLTPLAANILVSSLHWTLAPALV
                     IVLCSALAFAASFGMKREEPSADVLDANRNPRWQERR"
     gene            6352..8112
                     /locus_tag="VHV99_28650"
     CDS             6352..8112
                     /locus_tag="VHV99_28650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007744113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX3639380.1"
                     /translation="MNTPESVRIADVELNIQRYELRRAGEEIRLERLPMELLILMASR
                     NGQLVTRTDIVRALWGTNAYRETDNSINTAIRKIRVALQDDPVNPQHVITVKGKGYRL
                     DNVRPVSEESVPATQEAVRILVLPFQNQTGDSGQDSFCDALADETSANIGVLDPERIL
                     VISRTTAARYRRVSKSVAEIARELSLDYVLEGSLQRDGEKIRILAQLIRCVDQVQIWS
                     RAHEPMVSGALEIQKEVGTALAQEVSPALAEQQRHMLARRFPVDPAAHDAYLRGRYYW
                     TRRVHFDAGFAAHHALSDEDFVRARGYFERALERDPTYALGYVGISNVLGAASTHGVC
                     PPSEGHPRARQAALRALELDADLPEAHQALAGVHYFYDWDWHRAEEEFLEALRLNPSH
                     AETSRLYARLLLVLGREAEGRAQFDHAEKVDPLGCEGSRIFGLVQAGKYGEVIRECLS
                     GGHGNRSPLTYQLLVTAYEQQGQYEEAVEATIDALTRCNEFARAETIRTIWDAGGYKK
                     VLEWYLHDLMARHQRRYTSPFLFAEIYARLDQPDEMFHWLEVALAEHSPRLCELRTNA
                     WFHRYRSTGRFRNIEKRIGY"
     gene            complement(8238..8489)
                     /locus_tag="VHV99_28655"
     CDS             complement(8238..8489)
                     /locus_tag="VHV99_28655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007587650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639381.1"
                     /translation="MKTIRIVAVCAIGMLLGYAQAQDKPVPSGTPTSAQDVGGVTDAS
                     RSEMGMAMSPARQQAYPDLGKSQLSAEQSRGLSTTFAHH"
     gene            complement(8491..9021)
                     /locus_tag="VHV99_28660"
     CDS             complement(8491..9021)
                     /locus_tag="VHV99_28660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639382.1"
                     /translation="MVIDSANAFGDYAVKATDLENVGFHISDFSQRYGISQHKIEEGL
                     ATGGDIVSESGTDEIVLRSFFTVWMKARNKCYGNCWRSFAPRTLCEYRSAALQLDPRV
                     KLPGTAPSDQELNHAFASCCVNDFFRLCRWRRRRPDRSIEAAIATRRRILAGTVWDRL
                     TASHALTVSAPVDRRN"
     gene            9043..>9454
                     /locus_tag="VHV99_28665"
     CDS             9043..>9454
                     /locus_tag="VHV99_28665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016354126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX3639383.1"
                     /translation="MGESRLLWEIGVEGADIRTLRARLNLDSGYVSRTLASLERQKLV
                     AVLAHSGDRRVRRACLTQEGLVERAELERLSDAVALRVLEPLGAEQRRRLVAAMSEVQ
                     RLLQASLVHFALEEPDSDDARWCFEQYFGELDERF"
BASE COUNT         1724 a         2793 c         3022 g         1915 t
ORIGIN      
        1 acgaaaatgt agaagcagca gagaagattc agcagatgca ggcatatgcc caacgcggcg
       61 acccctctgg ggaccgctct tatgaacacc gcaaacctcg aatgagggcg gggcacgtac
      121 cagatgatca gcagcccgaa cgtcatgatg aaggcgcatt ccgcccattt cagccaggta
      181 ggacggcgca taaaccgtcc ctcgacaatc gtctcgatga cctgcgcctg aatttcgatg
      241 cccggcacca gctcccccaa cgccgtcgtg cgcatgtcgg tcacgccggc gccggtcagt
      301 ccgaccagca ccagtttgtc ccggatccgt tccggatcga ctttgccctg caggacgtcg
      361 cgtgcggaca cgtagcggct cagcgtcgag cggatggacg cgaaatgcaa ccagatatca
      421 ccgtcgggct gcgtgggcac ctggacatcc gcgacaccta ccgctcgcac gccggatgca
      481 tcggcaaaca catcgagcgc cgatgagccg gtggcgagac ggagcatttc catcggcaga
      541 ttggggacca gtttctcgct caaccccatg acgaggggaa tgtgccggac cacgccctgt
      601 tcgagcgcca cgctcagcaa cgcctgcccg tgcgctgccg cttgcagttc cggcaggctg
      661 gccagcacat agtcaaagcg ccttacgctc ccgatcgggt cgatgccatg cacgaggatc
      721 ggcgcgcttc gcagatcggt gctgctgccg aaggcggcat gatcgagcgc ggcggcaccc
      781 aggatggtcg gcgccgcgcg caacgatctg gcgagaatgg tttcgtgact gggcagggcc
      841 cgcaacccgg cggcgagtgc cgcggccgat ttcggcagat tggcggccac cttgtcgggc
      901 gaggtctggt ccggctccgg catgtaaatg tcgagaccga tcgccaacgg cttctgtgcc
      961 gcaatggcat cgatcaggtc cgcgagccga tttcgcggcc acggccattg gccgacggtg
     1021 gccagcgtct cctcatcgat ctcgacaatg gtcacgggct gagcggtcgg aatgcgcggg
     1081 aagcgccgtt gatactggtc gaacaaaagt tgccgggccg tttgaaagta gtccggcagc
     1141 gcctcgttca acgcgttcac ataagccgga cgtgctatgt cgctcggcca ttcgctgcac
     1201 aggttgagca ggctcaggcc aaacagcacc atcagtgcca tcggacgccc ctggaccacc
     1261 ttcagcagac gcctgatccg ggcaggccat gggccgtcat agcgtttcat cgaaggaaag
     1321 accatttagc gccgatgact ggcgcggtca ttggatggtg atgactgcgc gccgattcat
     1381 gcgatgggga acgccatctg ccgtggggac cagcggctcg cgcttgcccc gcgcggcggt
     1441 ctctatttgc cgggcaggaa tgcctgcctt gatcaggaac gccgcaacag tctgcgctcg
     1501 ctgcatggac agtctgtcgt tgaattcctg cgagcccgcc agatcggtgt gaccgaccac
     1561 cacgagctgc ggcgcaggcc acgtcgccag cacggctttt aacttctgga ccgtggcggt
     1621 ggactccggg gccaattcgg tggcggagtc gaagagaaaa aagatcgtga acgtcatggg
     1681 gcgaggaggc cgcgccgcga gcagctcgcc atatcgggtc tgcacgctgg cttggctgtc
     1741 catcgttttt tcgatggtgc cgccctttgt gacgtcgacc ccggcgtaag ccttgtcgat
     1801 ggtttgctct ccggtggcgc tgtggacagt gaccgcgccc actttgccct gcggatcagg
     1861 caataaaata atcttgtccg gcgtgctgca cccagccagg aaaaccagca tgacgcatgc
     1921 cgccgcagcg gccagcccga aacttcggat gacggccttc accgtccgct ctccccgtga
     1981 tcgccgactt cgatgataaa ttcggtgccg cgcacgccca aggtagtggt cggcgtgctg
     2041 aagcggaccg catccggatt cgcctttgcc agctttccgg aaatcacggc gagagatccg
     2101 cgtttgacgg tggcgtcgag taccccgtcg tgggtcgtgg tgttgaacgc gaatttgttc
     2161 agatcgagga tggtattggc gccggccgag agttgcgtgg tgtcacgcag cgtgatgccg
     2221 acggaggatt ccggaccggt cacgatgcga tcgctgccat aaacctcgct gccgatcgac
     2281 gcgccagaac ttgctccagc gcgctcgata tgtaccgcac ccttgagggt cttgactaca
     2341 ccggcggcgt cgtcggcacg tgcgtcggcg gcagccatgt agccacaggc aaggatcagg
     2401 agatacttga gggatcggag acgcatcggc ggcacctgaa gggaaatggc ttagcatttc
     2461 tgcgccactg gctagtacat aagcgctcca gcggatcaat atagcttggt tgaccattct
     2521 gccgagttaa tcatgcggcc acttcaggaa atacccgtat aggtatgtcg attcgctcac
     2581 gaatggcgcg caatggcgaa cgagcgccgc ggtgcgattg catgttgtgc ttcaacggcg
     2641 catcggtttg gcggcgtcgc gttcatccct tcgcgtgcgc agcatgtgct gcgtgatgca
     2701 acggtgctca agctgcatgg caaagaggcg aagtggcgcg tgggactcgc atggccatcc
     2761 acgtgcgtga atcctgttac gacgcgcttt gtgtcgttcg tgcgcccggc gatgaaaggc
     2821 gcgctggccg cgcaggatta acgcccggtt atcgcgcgca tgaatcgcgc gtgccgggtg
     2881 aaatcacagg ctcgcgtgga ttgtcgagcc ggatcatctg accgtgatgc agcaccgtga
     2941 acgcgtcgtc gggcaggccg gcttcacgtg cggccgcggc gagttttttg ggcggttcgt
     3001 cgagcggctc gtcggcgagt tcgaacgttc cccagtgaat gccgattgct tttttcgcat
     3061 gcacgtcctg gaaaatctgc acggcctgct gtgggtcgac gtgttgcggg gccatgaacc
     3121 agcgcggcgc atacgcgccg atcggaatca gcgcgaggtc gaagcagccg aacgccgcgc
     3181 cgatccgctt cgcgtcgccc gagtagccgg tgtcgccagc gaaatacagc gagaacggat
     3241 gcgctgcgcg cggcggcgtc ttcatgaccc agccgcccca tagcgtctcg ttgcggtcgg
     3301 tgagagtgcg cgcggaccag tgcgtcgcgg gcacgaacca gaagtcgagc tcggatgctt
     3361 gcgtccggtc accccagtcc agttcttgtg catgggtgat gcctttctgc gcgagccatt
     3421 ccttgatgcc gagcggcacg agaaagagcg gcgggccgcc gggctggccg ttcaatgcct
     3481 gaacgcttgc cgcgtcaagg tgatcgtagt ggctgtgtga gatcagcacg acgtcgatat
     3541 gcggcaattc ggcgagcgtc agaccggggg gcacgcgccg cttcggcccg atgaagggga
     3601 acggcgacgc gcgttcggaa aacaccggat cggtcaggat gttgacaccg tcgatttgca
     3661 gcagcgcgct cgagtggccg atccacgtca tcgtattctc gctgcggttc gccttgatcc
     3721 attcgacgtc cggatgatcc acgggaaacg cgtagccgct ttcgggcggc ggcggcagat
     3781 gatgaatgag acggttccag cgccacttcc agactgaacc ccgcgcgagc gggccctcgt
     3841 cgttttcgaa accgttatcc gtgtgcggcg cgtgatggat gccgctggcg gaaaggcgct
     3901 cgggtgacgg atcgggttct tgtgcgctgg cggcgcaggg tgccgttgcg gcgtagagcg
     3961 cgaagacggc ataaagcaga ggcaggcgtg atgaatatgg catcgcaaac aggacgaagt
     4021 ggctgagctt ccgttgcatg gacgtttcag atgccacagt gtagaccggc tgaaaaaagc
     4081 tcagcatgcg ctgctagaat cgacacgtcg tttgcgtctt gggcaacatg cacggcggcg
     4141 gagcggctgc gggccgatgc aagtcgtccg cgcgcgccac gggcaccccg tcgttcgcga
     4201 agggaaccac ggcgcaagtt ggtcgaaagg ccctgggaag cacgctaacg gtgcctacgg
     4261 tggcgcggcc gcaaaaacgg cattgacggg aatacatcat gtccaccgag tggaaacgca
     4321 gcttcaaact gtttatccag cgtttctggc aaccgaccag cgcgtgtatg acctgcatgc
     4381 cgggaagttg gggcaatgtg atgagccttg cccactggac gattgcattc cacacaggtc
     4441 tgctcaccgg gctcctcgcg gtctttttga tcttcacgcc tgcggcgaaa ctctacacac
     4501 atcgatacgg caatgcgctg atcgtcggct gcctgacagc aataggcgat gcctacgcgc
     4561 acgcgagtca ctacggcatc ccctacgtcg agcacattgt gacgggtgct atttcagcgc
     4621 tcttgacgct ggccggctca tatctcttcg aagatcgtgc gcgacgcctt cgggcggttt
     4681 gggcgcgggt tttcggatag cgccttcggc catcttcgga ggttggccca cgctttcaca
     4741 cgggcctgac tccgcaggcg atgcagggat ggacggttgc acgccagcgc gaagcatagt
     4801 ggccgtcgaa ctggcgtaat ggattgagct agatccgggc tctgcatctc tctgtaccac
     4861 aagacgaaac taaagacctt tcatacaaat cgtttgtcgt ccatcatatt tgagccggaa
     4921 cgatcgcgcg ccatccggaa gtcagattga catggtggtg gctgaacccg tctgtccggc
     4981 gcgacgtccg cttaaagtgg tcgttgtacc ttgcgatatc agggatgcca tgctcgacga
     5041 ttcgctcccc atccgaaagg cgttcatgac agcgcgccaa cgctggacca ttctggtcgc
     5101 cagtaccggg ggagcgctgg aggtattcga tttcgtcatc ttcggatttt tcgcacgaac
     5161 catcggcagt gagttctttc catcacaaac cggcatatcg gcggagacct tgtcgttttc
     5221 ggtgctggcc attggctacc tttccaggcc gttgggaggc atcttccttg gccggttagg
     5281 cgacacgcat ggccggcgcc tggttttcgt gtcgtccgcg atgatcgcct ccatttcgac
     5341 actcctcatt ggaatacttc cttcatacgc ggtatggggc gcggccgccc cggcgcttct
     5401 tctcgtgttg cggctcatcc agggtttctg cctcggcggt gagctgccag gcgcagtggt
     5461 ctatgccgtt gaaaccgtgc gtggaaggcc tggtttcctg tgcggcatcg tgttcgtcgc
     5521 ggtcaacctt gcgttgcttc tggcgacgag cattaatctt gcagtgcaat gggcgttcac
     5581 acccgagcaa gtcaacgcgt tcggatggcg gatcggcttt ctgtttggtg ggatggtcgg
     5641 gctgttgagc tttgccctgc gccggaccct tgctgaaacc gacgaatacg cgcgcgccat
     5701 tggcgcccgg catcgcgagc ctcttgccgt cctgtttcgc aaccatttcg ctcgtttgac
     5761 gacaggcgct gccgcgtgtc tgctggtggg tgcgtcgggt ggcatcttta tcggattcgc
     5821 gccgggatat ctgcaagggc ttcaatacga cgcgcagcag atcgcgagtg cccagacgcg
     5881 ttacctgatc gtcgtggcag tctgcattct cgtgacgtcg tacgttggcg acctgctgcc
     5941 gcgccgttat gttttcaggt cgggcgccgt gttgtccgcg ctgttcgccc ccttcttctt
     6001 catggcggct gcccgtcact acgtcagtct gtccgtgctg ttcgtgacgg ccggcgtcgt
     6061 cacctcgctc gccaatggaa ccttcgcgtg cgcgcttgcc gacatgtttc cagtcgacgt
     6121 ccggttcagc ggcgttgcca cggccatgaa tctcggtctg gccacgtcgc tgggactgac
     6181 gccgctcgcg gcgaatatcc tcgtttccag tctgcactgg acgctcgcgc ccgctctcgt
     6241 cattgtgttg tgctcggcgc ttgcattcgc ggcttcattc gggatgaagc gcgaggaacc
     6301 gagcgctgac gtcctcgatg cgaacagaaa cccgcgatgg caggagcgca gatgaacacg
     6361 ccagaatccg tcaggatcgc ggatgtcgaa ctcaacattc agcgctacga attgcgtcgt
     6421 gcaggcgagg agatcaggct tgagcgtttg ccgatggagt tgctgatcct gatggcatca
     6481 cgtaacgggc aactcgtcac gcgtacggat atcgtgcgag cgctgtgggg cacaaatgcg
     6541 tatcgggaaa cggacaacag catcaatacg gcgatccgga aaattcgcgt cgcgcttcag
     6601 gatgatccgg tcaacccgca gcacgtcatc acggtgaagg gcaaaggcta tcgcctggat
     6661 aacgtgcggc ccgtgtcgga ggaatcggta cccgccacgc aggaagccgt gcggatactg
     6721 gtccttccgt tccagaacca gactggcgat tccggccagg acagtttctg cgacgcgctg
     6781 gccgacgaaa cctccgccaa catcggcgtg ctggatccag agcgcattct tgtcatttcg
     6841 aggacgaccg ccgcccgcta ccggcgggtc agcaagagtg tcgcggagat cgcgcgcgag
     6901 ctgtcgctcg actacgtgct ggaaggttcg ctccagcggg acggcgagaa gattcgcatt
     6961 ctggcgcaac tgatacgttg cgtcgatcag gtgcagatct ggagtcgcgc gcatgagccg
     7021 atggtgagcg gagcgctgga aatccagaaa gaggttggaa cggcgctcgc ccaggaggta
     7081 tcgccagcgc tcgcggagca gcagcggcac atgctggcga gacgttttcc ggtcgatccg
     7141 gcggcgcatg atgcctatct gcgcggccgt tactactgga cccgtcgcgt gcacttcgac
     7201 gcggggtttg ccgcgcatca cgcgttgagc gatgaggact tcgttcgcgc gcggggctac
     7261 ttcgagcgcg cgctcgaacg ggatccgact tacgcgctcg ggtatgtggg gatctcgaac
     7321 gtcctcggcg ccgcatcgac acatggggtc tgtccaccgt cggagggcca cccgcgagcg
     7381 cggcaggcgg cgctgcgagc actggagctg gacgcggatc ttcccgaggc tcaccaggcg
     7441 ctagccggcg tgcactactt ttatgactgg gactggcatc gggcggagga ggagtttctc
     7501 gaggcgttac ggctcaaccc gagccacgcc gaaacgagcc gcctgtatgc aagactcctg
     7561 ttggtgctcg ggcgagaagc ggaaggcaga gcgcagttcg accatgccga gaaggtcgac
     7621 ccgctcggct gcgaagggtc acgcatattt gggctcgtac aggcgggcaa atacggcgag
     7681 gtgattcggg agtgcttgag cggtgggcat ggcaaccgct cgccgctgac ctatcagctg
     7741 ctggtcaccg cctatgaaca gcagggccaa tatgaggagg ctgtcgaagc gactatcgat
     7801 gcgctaacgc ggtgcaacga attcgcccgc gcggagacga ttcgcacgat ctgggacgcc
     7861 ggaggctaca aaaaggtcct ggaatggtat ttacatgact tgatggcgag gcatcaaagg
     7921 cgctatacgt cgccgtttct gtttgcggaa atttacgcgc ggctcgacca gccggacgag
     7981 atgttccatt ggctcgaggt tgcgcttgcc gaacattcgc cgcgcctgtg tgaacttcgc
     8041 accaacgcat ggttccatcg ctatcgctcg acggggcggt tccggaacat cgaaaagcgc
     8101 attggctact gagcctggga gcgaagccgc gtgacgcggc gcacgagtcc cgtatgccag
     8161 ccaccgttac gcacgatgag tgtttcgcgt gcattcatcg tgcgggacgg cggtcgcgca
     8221 aagccggcgc gcagcagtca atggtgggcg aacgtcgtgc tcaagccgcg gctttgctct
     8281 gcggatagct gggatttgcc gaggtccggg taagcctgtt gccgtgctgg actcatggcc
     8341 attcccattt cacttcttga ggcatcggtg acgccgccga catcctgagc ggacgttgga
     8401 gtgcccgacg gcacgggctt gtcttgcgcc tgcgcgtatc cgagcaacat gccgatcgcg
     8461 cagactgcca caattcgaat cgtcttcatg tcagttcctc ctatccactg gtgcggaaac
     8521 ggtcagcgca tgactggccg taaggcgatc ccagacggtt cccgcgagaa tgcggcgtcg
     8581 cgttgctatc gctgcttcaa tactgcgatc gggccgcctt ctgcgccatc gacataacct
     8641 gaagaaatcg ttaacacagc agctcgcgaa tgcgtggttc agttcctggt cgctcggcgc
     8701 agtgccgggc agtttcaccc gggggtcgag ctggagtgcg gcgctgcggt attcacacag
     8761 cgtgcgcggg gcaaacgagc gccagcaatt cccgtagcat ttgttccgtg ctttcatcca
     8821 gaccgtgaag aacgagcgca agacgatttc atcggtgccg gactccgaca cgatgtcgcc
     8881 accggtagcc agtccttcct cgattttatg ttgactgatt ccatatctct gactaaagtc
     8941 agagatatgg aatccgacat tctccaggtc cgtagcttta accgcatagt cgccgaaagc
     9001 attggcgcta tcgatgacca tttcctcgga cggggccgcc cgatgggtga atcacgcctg
     9061 ctatgggaga tcggtgtcga aggtgcggat attcgtactc ttcgcgcacg gctaaatctt
     9121 gattcaggtt atgtcagccg tacgttggcg tctcttgaac ggcaaaaact tgtcgctgtc
     9181 ctcgctcatt cgggcgaccg ccgggtacgc cgggcttgcc tgactcagga gggcctcgtg
     9241 gaaagggctg aactcgaacg gctctccgac gcggtcgctt tacgcgtgct cgaaccgctc
     9301 ggcgctgagc agcgtcggag actggttgca gccatgtctg aggttcagcg tttgctgcag
     9361 gcatcgctcg tgcattttgc gctcgaggaa ccggatagcg atgacgcgcg gtggtgcttt
     9421 gaacagtatt tcggcgaact cgatgagcgc tttg
//
LOCUS       DATASH010001082         9185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2365833,
            whole genome shotgun sequence.
ACCESSION   DATASH010001082 DATASH010000000
VERSION     DATASH010001082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 9185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9185
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2365833"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..1480
                     /locus_tag="VHV99_28670"
     CDS             194..1480
                     /locus_tag="VHV99_28670"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HEX3639384.1"
                     /translation="MRVVVLGSGVVGVTSAYYLARAGHEVTVIDREAGPALETSFANA
                     GQISPGYASPWAAPGVPLKAVKWIFQKHAPLAIRLDGTQFQLQWMWQMLQNCTSSRYA
                     VNKGRMVRLAEYSRDCLQALRAETGIQYEGRTGGTLQVFRTQQQFEGAAKDIAVLQEA
                     NVPYELLSPAELARAEPALAAVSHKLTGGLRLPGDETGDCQMFTTRLAALAEQFGVKF
                     RYNTPIDALAMAGERIAGVQCGNELVRADSFVVALGSYSTQFLSGLVKIPVYPLKGYS
                     ITAPIINEASAPISTVLDETYKIAITRFDNRIRVGGMAEIVGFDKSLRQARRETLEHC
                     VNDLFPGGGDTSKATFWTGLRPMTPDGTPIVGRTPVPNLFLNTGHGTLGWTMSCGSGQ
                     LLADVMSGKQPAIKADDLSVHRYVGETGGAHRPAYA"
     gene            complement(1623..3413)
                     /locus_tag="VHV99_28675"
     CDS             complement(1623..3413)
                     /locus_tag="VHV99_28675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX3639385.1"
                     /translation="MSYTAPIKDMLFVMKELARLDDIATLPGFEDANLDTAQAVLEES
                     AKLCGEVLAPLNVEGDRNPSSWKDGVVTATPGFKDAFRQFGEGGWQGVQHPVDYEGQG
                     LPKLIATACIEMLNASNLSFALCPLLTDGAIEALLTAGSEEQKQTYVPKLISGEWTGT
                     MNLTEPQAGSDLALVRTRAEPQGDGSFKLFGTKIFITWGEHDMAKNIAHLVLARTPSA
                     PEGVKGISLFIVPKFLVNEDGSLGERNDVHCVSIEHKLGIKASPTAVLQFGDHGGAIG
                     HLIGEENRGLEYMFIMMNAARFAVGMQGVALSDRAYQRAVAYAKDRVQSRPVDGSAKQ
                     SVAIIQHPDVRRMLATMRGLTEASRALAYVAAAHCDIAHRHPDEAKRAEHQAIYEYLV
                     PIVKGWSTELSIDVTSLGVQVHGGMGFIEETGAAQYYRDARILPIYEGTTAIQANDLI
                     GRKTVRDGGKVAKSLLAGVAETIEALGAQQGAAFESMKKYLEEGHRSLGAVVDFVVTN
                     TKSDPNAVFAGSVPYLKLAGIVLGGWQMARALIVAAEKRDEDPSFYGAKIATAQFYAE
                     HVLPLASALEASIVSAKGGEGVLALSEDQF"
     gene            complement(3517..4452)
                     /locus_tag="VHV99_28680"
     CDS             complement(3517..4452)
                     /locus_tag="VHV99_28680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006414355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX3639386.1"
                     /translation="MTNLVIAEHDNASIKAATLNTIAAAQKIGGDIHVLVAGHNAQAA
                     ADAAAKIAGVSKVLLADAPQLEAGLAENVEATVLNIAKDYTHILAPATAYGKNIAPRI
                     AAKLDVAQISDITAVDSTDTFERPIYAGNAIATVQSADPIKVVTVRSTGFDPVAAEGG
                     SATVEKMEAAADTGLSQFVSREVTKLDRPELTSAKIIVSGGRGLGNGENYTKVLEPLA
                     DKLNAALGASRAAVDAGFVPNDYQVGQTGKIVAPQLYVAVGISGAIQHLAGMKDSKVI
                     VAINKDPEAPIFSVADYGLVGDLFTVVPELVSELG"
     gene            complement(4466..5215)
                     /locus_tag="VHV99_28685"
     CDS             complement(4466..5215)
                     /locus_tag="VHV99_28685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEX3639387.1"
                     /translation="MKILVPVKRVVDYNVKVRVKSDGTGVDIANVKMSMNPFDEIAVE
                     EAVRLREAGVATEVIAVSAGVTQSQETLRTALAIGADRAILIESSEELQPLAVAKLLK
                     ALVDKEQPSLVILGKQAIDDDSNQTGQMLAALANLPQATFASKVVVADGKATVSREVD
                     GGAETLSLKLPAVVTTDLRLNEPRYVTLPNIMKAKKKPLEVIKPEDLGVDVTPRLKTL
                     KVVEPPKRSAGVKVPDVKTLVEKLKTEAKVL"
     gene            complement(5287..6546)
                     /locus_tag="VHV99_28690"
     CDS             complement(5287..6546)
                     /locus_tag="VHV99_28690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3639388.1"
                     /translation="MEINSFSRQGLIAHDRVWIAAFVFAFLGLMVDGADLMFLSYSLS
                     SLKREFGLSNVEAGALGSITLAGMAIGGIYGGWACDRFGRVRTVVWTIAVFSIGTAAL
                     GFTHTFSQFAAFRFIASLGLGAQYVACNTLMSEYSPTRFRSTILGTLQAGWSVGYVVA
                     TILAAWILPTFGWRYLFFISIVPVVLALIMQRIVPEPESYVASRDARRRGDAAGKESS
                     EGTMKLVFGDQAHRRMFMLWSLTAGLLQFGYYGVSTWMPTYLEKELHLNFSSMTSYMV
                     GTYVAMILGKVLAGWLADKFGRRAVFAFGAFGAAVFLPAIVLWHTPGNIVYLMTFFGL
                     LYGMPYGVNATYMTESFEARIRGSAVGGAYNLGRAGAAIAPAAIGFLATNGTIGLGFL
                     VMGGAYFLCGLVPALFIREKLYDPQNA"
     gene            complement(6660..7814)
                     /locus_tag="VHV99_28695"
     CDS             complement(6660..7814)
                     /locus_tag="VHV99_28695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007744755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX3639389.1"
                     /translation="MIRDHETLILLLGAVSRFVRERLVPAEDQVAQSDQIPAGIVAEM
                     RELGLFGLSIPEEYGGLGLTMEEEVLVAFEIGKTSPCFRSLFGTNVGIGSQGIIIDGT
                     AEQKQHYLPRLASGELIASFALTEPGSGSDAASLSTRAVRDGDHYVIDGTKRFITNAP
                     EAGIFTVMARTNPDVRGAGGISAFIVERGTPGVSLGKIDKKMGQKGAHTCDVIFENCR
                     VPVQNLIGGIEGIGFKTAMRVLDKGRLHIAAICVGAAERMLDDALHYAMERQQFGQPI
                     GEFQLVQAMLADSKAEIYAAHSMVLDAARRRDNKEDVSTQASCCKLFASEMCGRVADR
                     AVQIHGGAGYVSEYAIERFYRDVRLFRIYEGTSQIQQLVIARNMIRSASI"
     gene            complement(7866..8651)
                     /locus_tag="VHV99_28700"
     CDS             complement(7866..8651)
                     /locus_tag="VHV99_28700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX3639390.1"
                     /translation="MARFDEEAGTDRQFVTALARGLEVLRCFGPGERYLGLSELARRT
                     SLPKATVARLAGTLAKLGYLRYSEPLGKYSLGTGVLSLGYSMLSNLDIRDVARPFMQE
                     LAEYARASVAVGVRDRLSIVYIEWVRSSAPITVMRAVGVRLPLATTSIGRAYLAAAPE
                     DERNEVLEQVRLQDESDWPRIKRGIDQAVKDYEERGFCLSVGEWDKDIASVGAPFTAP
                     DGTRMAFNCGGPEFVMSRDTLENDIGPRLVDLVQRLGAAIGRR"
     gene            9051..>9185
                     /locus_tag="VHV99_28705"
     CDS             9051..>9185
                     /locus_tag="VHV99_28705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008645289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX3639391.1"
                     /translation="MSRALSHLRILDLSRVLAGPWASQTLADLGAEVIKIERPGTGDD
                     T"
BASE COUNT         1796 a         2946 c         2624 g         1819 t
ORIGIN      
        1 ggaccggttg gcgctgtgta cgcatggtgt ttttgctgaa aatcttgaaa atacaataac
       61 aaaacctacc tgactgtcaa tagtatagcg gttaacactg ggcgagccgc attacacctc
      121 gttaatcgaa cgtattccaa cgaatcgacg agcgcaccgc cctcagatcg cttattagat
      181 acctggagca gctatgcgag tcgtcgtttt aggcagtggc gtcgtcggcg tgacgagtgc
      241 ttattacctg gcacgcgccg gtcatgaagt cacggtgatc gatcgcgagg cgggtccggc
      301 gctcgaaacc agctttgcca atgccggcca gatctcgccg ggctacgcgt cgccgtgggc
      361 ggcgccgggc gtgccgctga aggccgtcaa atggatattc cagaagcatg cgccgctcgc
      421 gatccgcctt gacggcacgc aattccagtt gcagtggatg tggcaaatgt tgcagaactg
      481 cacgtcgtcg cgttatgcgg tgaacaaggg ccgcatggtt cgtctcgctg aatacagccg
      541 cgactgtctg caagcgctgc gcgctgaaac cggcatccag tacgaagggc gcaccggcgg
      601 cacgctccag gtgttccgca cgcagcagca attcgagggt gcggcgaaag acatcgcggt
      661 gctgcaggaa gccaacgtgc cgtatgaact gctgtcgccg gccgaactcg cgcgggccga
      721 accggcgctc gccgccgtgt cgcacaagct gaccggtggt ctgcgcctgc cgggcgacga
      781 aaccggcgac tgccagatgt tcaccacgcg cctcgccgcg ctggccgagc aatttggcgt
      841 caagttccgc tacaacacac cgatcgacgc cctggccatg gcgggcgagc gcatcgccgg
      901 cgtgcaatgc ggcaatgaac tggtgcgcgc ggattcgttc gtcgtggcgc tgggttcgta
      961 ctcgacccaa ttcctgtccg gtctcgtgaa gattccggtc tacccgctca agggctattc
     1021 gatcaccgcg ccgatcatca acgaagcgtc cgcgccgatt tcgaccgtgc tcgacgagac
     1081 ctacaagatc gcgatcacgc gcttcgacaa ccggattcgc gtcggcggca tggcggagat
     1141 cgtcggcttt gataagtcat tgcgtcaggc gcgccgcgag acgctggaac attgcgtgaa
     1201 cgatctgttc ccgggcggcg gcgatacgtc gaaggccacc ttctggaccg gcctgcgtcc
     1261 catgacgccg gacggcacgc cgatcgtcgg ccgcacgccg gtaccgaacc tgttcctgaa
     1321 tacggggcac ggtacgttgg gctggacgat gtcgtgcggc tcgggtcaat tgctagccga
     1381 cgtgatgtca ggcaagcaac cggcgatcaa ggcggacgac ctgtcggtgc atcgctatgt
     1441 tggcgagacc ggcggtgcgc atcgtccggc ttatgcttga ggtattacgc tttacgcttc
     1501 gcgagcggtt gagccggtcc gttacagcgg tccggcctga caaaagcaaa cggcgcctta
     1561 ccgggcgccg tttgtttttt tacgcatgag ctttcgctac atcgctcgag acgggtacaa
     1621 catcagaact gatcttccga caacgccagc acaccctccc cgcccttcgc gctaacgatc
     1681 gacgcttcca gtgccgaagc cagcggcagc acatgctcgg catagaactg agcggtcgcg
     1741 atcttcgcgc cgtagaacga cggatcttcg tcgcgctttt cagccgccac gatgagcgca
     1801 cgggccatct gccagccgcc cagcacgatg cccgccagct tcagatacgg cacgctgccg
     1861 gcaaacaccg cgttcggatc gctcttcgtg ttggtcacga caaaatcaac cacggcgccc
     1921 aacgagcggt gaccttcctc cagatatttc ttcatcgatt cgaacgcagc gccttgttgc
     1981 gcgccgagtg cctcgatcgt ttcagccaca ccggctagca gcgacttcgc caccttgcct
     2041 ccgtcacgca cggtcttacg gccgatcagg tcgttggcct ggatcgcggt cgtgccttcg
     2101 tagatcggca gaatgcgcgc gtcgcggtag tactgcgcgg cgccggtttc ttcgatgaaa
     2161 cccatgccgc cgtgcacctg cacgccgagg ctcgtcacgt cgatcgacaa ctccgtgctc
     2221 cagcccttca cgatcggcac gaggtattcg tagatcgcct gatgttcggc gcgttttgct
     2281 tcgtcaggat gacgatgcgc gatatcgcag tgagccgcgg cgacatacgc caacgcgcgt
     2341 gatgcttcgg tgaggccgcg catggtcgcg agcatgcggc gcacgtccgg atgctggatg
     2401 atcgccaccg actgcttcgc ggaaccatcc accggccggc tttgcacgcg gtctttcgcg
     2461 tatgcgacgg cccgctgata cgcgcgatcc gacagggcga caccctgcat gcccaccgcg
     2521 aaacgcgccg cgttcatcat gatgaacatg tattcgaggc cgcgattttc ctcgccaatg
     2581 aggtggccaa ttgcgccgcc gtggtcgccg aattgcagca ctgcggtcgg gctcgccttg
     2641 atgccgagct tgtgttcaat cgacacgcaa tgcacgtcgt tgcgctcgcc gagcgagccg
     2701 tcttcgttga cgagaaactt cggcacgatg aagagcgaga tgcccttcac gccttccggt
     2761 gcgcttggcg tgcgcgccag cacgagatgg gcgatgttct tcgccatgtc gtgctcgccc
     2821 cacgtgatga aaatcttcgt gccgaacagc ttgaacgaac cgtcaccctg cggctccgcg
     2881 cgcgtgcgca ccagcgcgag atcggagccg gcttgcggct cggtcagatt catcgtaccg
     2941 gtccactcgc cggaaatcag cttgggtacg taggtctgct tctgctcttc gctgccggcc
     3001 gtgagcagcg cctcgatggc gccatccgta agcagcgggc acagcgcgaa cgacaggttc
     3061 gaggcattca gcatctcgat acaggccgtg gcgatcagct tgggcaggcc ctggccttca
     3121 tagtcgactg gatgttgcac gccctgccag ccaccctcgc cgaactggcg gaaggcgtcc
     3181 ttgaaaccgg gcgttgcggt gaccacgcca tccttccagc tgctgggatt gcgatcacct
     3241 tcgacattca gcggcgccag cacttcgccg cacagtttcg ccgactcttc gagtacggcc
     3301 tgcgccgtgt cgagattcgc gtcttcgaag cccggcagcg tcgcgatatc atcgagacgg
     3361 gccagttctt tcatcacgaa cagcatgtcc ttgatgggcg ccgtatagct catttcagtt
     3421 ctcctcctgc ctatcgataa aaaaagggcg ctggacttga tcggtcccgt gccctttcgt
     3481 gtgctgccag acgcctttta accggcgctg cggcgcttaa ccgagctcgc taacgagttc
     3541 gggtacgacc gtgaacagat cgcccaccag accgtaatcg gcgacgctga aaatcggcgc
     3601 ttccgggtcc ttgttgatcg cgacgatgac cttcgaatcc ttcatgccgg ccagatgctg
     3661 gatcgcaccc gagatgccga cggccacgta cagttgcggc gcgacgatct tgcccgtttg
     3721 gccgacctga tagtcgttcg gcacaaagcc cgcatcgacc gcggcgcgcg atgcgcccag
     3781 tgctgcgttc agtttgtcgg ccagcggctc gagaaccttc gtgtagttct cgccgttacc
     3841 cagaccgcgg ccacccgaga cgatgatctt cgccgacgtc agttccggac ggtccagctt
     3901 cgtcacttca cggctcacga actgcgagag acccgtatcg gccgcggctt ccatcttctc
     3961 gaccgttgcg ctgccgcctt ctgccgcgac tgggtcaaaa ccggtcgagc ggaccgtgac
     4021 gaccttgatc gggtcagccg attggaccgt cgcgattgcg ttgcccgcgt agatcgggcg
     4081 ctcgaacgtg tcggtgctgt ccactgcggt gatgtcgctg atctgtgcga cgtccagctt
     4141 cgctgcgata cgcggtgcga tgttcttgcc gtaggcggtc gccggcgcga ggatgtgcgt
     4201 gtagtccttc gcgatgttca gcaccgtcgc ttcgacgttt tctgccaggc ccgcttcgag
     4261 ttgcggcgcg tcggccagca ataccttcga gacaccggca atcttcgctg ccgcgtcagc
     4321 cgcagcctgt gcgttgtgac ccgcgaccag cacgtgaatg tcaccgccaa tcttctgcgc
     4381 cgctgcgatc gtgttcagcg tcgcggcctt gatcgacgcg ttgtcgtgtt ctgcaattac
     4441 cagattcgtc atttcgtccg tctccttaca gcaccttggc ttcggtcttc agcttctcga
     4501 ccagcgtctt cacatccggc accttcacac cggcggagcg cttgggcggc tcgacgactt
     4561 tcagcgtctt caggcgcggc gtcacgtcaa caccgagatc ttccggcttg atgacttcca
     4621 gcggcttttt cttcgccttc atgatgttcg gcagcgtcac atagcgcggc tcgttcaggc
     4681 gcaggtcagt ggtgacgact gcgggcagct ttaaagacag cgtttcagca ccgccgtcca
     4741 cttcgcgcga cacggtagcc ttcccgtctg ctacgacaac tttcgacgcg aacgtggctt
     4801 gcggcaggtt agccagcgca gccagcatct gcccggtctg gttcgaatcg tcgtcgatcg
     4861 cctgcttgcc gagaataacc aaagaaggct gttccttgtc gaccagcgcc ttcagcagtt
     4921 tggcaaccgc cagcggctgc aactcttcag aagactcgat caggatcgcg cggtccgcgc
     4981 cgatcgccag cgccgtgcgc agcgtttcct gcgactgcgt cacaccggcc gacacggcga
     5041 tcacttcggt cgccacgcct gcttcacgca gacgcacggc ctcttcaacc gcgatttcgt
     5101 cgaacggatt catcgacatc ttcacgttcg cgatgtcgac gcccgtgccg tccgacttga
     5161 cccggacttt cacgttgtag tcgactacgc gctttactgg caccagaatc ttcataaaat
     5221 ccccaatcaa caaatatgcg gccgccccgc aagctgagca ggcacggccg cggtcagtcg
     5281 ttacggtcac gcattctgcg gatcgtagag cttctccctg atgaagagcg cgggcacaag
     5341 accgcaaaga aaataggccc cgcccatgac cagaaaacca aggccaatcg ttccgttcgt
     5401 ggcaaggaat ccaatcgcgg caggggcgat cgccgcaccg gcacgtccca ggttgtatgc
     5461 gccgccgacg gccgagccgc gaatgcgggc ttcgaaactt tcggtcatat aagtcgcgtt
     5521 cacgccatac ggcatcccgt aaaggaggcc gaaaaaggtc atgagataaa caatattccc
     5581 gggcgtgtgc cacaacacga tagccggcaa gaacaccgcc gcaccgaatg caccgaatgc
     5641 gaataccgct cgccggccaa acttgtccgc gagccagcct gccaggacct ttccgaggat
     5701 catggcgaca taggtgccga ccatatagct cgtcatcgac gaaaaattca gatgcagttc
     5761 cttctcgagg taggtcggca tccaggtgct gacaccgtag tagccgaact gcagcaggcc
     5821 tgccgtcagc gaccacaaca tgaacatgcg acgatgagcc tgatcgccga agacgagctt
     5881 catcgtaccc tcgctgcttt cttttccggc ggcgtcgccg cgacgacgcg cgtcccgaga
     5941 agcaacgtaa ctctccggct cgggaacaat gcgctgcata atcaacgcaa ggacgacagg
     6001 aacaatcgag atgaagaaaa gataacgcca gccaaacgta ggcagaatcc aggcggccag
     6061 aatagtcgcc acgacatagc ccactgacca ccctgcctgt agcgtaccga ggatcgtcga
     6121 acgaaatcgg gtaggcgaat attcggacat cagtgtattg cacgcgacat attgagcgcc
     6181 gagcccaagc gatgcaatga agcgaaatgc ggcgaactgc gagaaggtat gcgtgaagcc
     6241 gagtgcggct gtaccaatcg aaaacaccgc gatagtccag accaccgtgc gcacgcggcc
     6301 gaagcgatca caggcccacc cgccgtaaat gccgccgatc gccatgcccg ctagcgtgat
     6361 gctaccgagc gcccccgctt ctacattcga gagcccgaac tcgcgcttga gactcgacag
     6421 gctataagac aggaacatca ggtctgctcc gtcgaccatc agaccgagaa aagcaaatac
     6481 aaacgccgcg atccatacac gatcgtgggc gatcaagccc tgccggctaa acgaattgat
     6541 ttccatctac acgcctccat gctacaaaca tgccgcaagc gctctctggt gcttttacgg
     6601 caaacgattt gcggtgatca gtcaccgctc acttcactat ttcgacacag cggcagcgat
     6661 cagatacttg cgctgcgtat catgtttcgc gcgatgacaa gttgctgaat ctggctcgtg
     6721 ccttcgtaga tacggaacag gcgcacatcg cggtaaaagc gttcgatcgc atactcggag
     6781 acatatcccg cgccgccgtg gatctgcacg gcgcgatcgg ctacacgtcc acacatctcc
     6841 gaagcgaaca gcttgcagca cgatgcctgc gtcgatacgt cttccttgtt gtcgcggcga
     6901 cgcgccgcgt cgagcaccat cgaatgggcg gcgtagatct ccgccttgct gtcggcgagc
     6961 atggcctgaa caagctggaa ttcgccgatc ggctggccga actgttgccg ctccatcgcg
     7021 tagtgaagtg catcgtcgag catgcgctca gccgcgccaa cgcagatcgc agcaatgtgc
     7081 agacgccctt tgtccagcac cctcatcgcg gtcttgaagc caatcccctc gataccgccg
     7141 atgaggtttt gcacaggcac tcgacagttt tcgaagatga cgtcacacgt atgtgcgccc
     7201 ttctgcccca tctttttgtc gattttcccg agcgataccc cgggggtgcc tctttcgacg
     7261 ataaatgcgg agatgccgcc cgcgcctctc acatcgggat tggtgcgagc catgacggta
     7321 aagatgccgg cttcgggcgc gttcgtgatg aagcgcttgg tgccgtcgat gacatagtgg
     7381 tcaccgtcgc gcacggcccg cgtagacagc gaagccgcat ccgaaccgga acccggctcg
     7441 gtcagcgcga acgaggcgat gagttcaccc gacgcgagcc gcggcagata gtgttgcttc
     7501 tgttctgccg ttccatcgat gatgatcccc tgcgagccga taccgacatt ggtgccgaac
     7561 aacgagcgaa agcatggcga cgttttcccg atttcaaacg ccaccagcac ttcctcttcc
     7621 atcgtgagcc caagaccacc gtattcctcg gggatggaaa ggccgaaaag cccaagctcg
     7681 cgcatctcgg ccacgatgcc agcgggaatc tgatcgcttt gcgccacttg atcctcagcg
     7741 gggacgagcc gctcgcgcac aaagcgcgaa actgcgccga gaaggaggat tagcgtttca
     7801 tggtcacgga tcatctgtat tcctgattcc agaagggcgc ttcgtgcgag cgccctatgt
     7861 gtcggtcatc gccgccctat ggcggcacct aaacgttgca caaggtccac gaggcgcgga
     7921 ccaatgtcgt tttccagcgt gtcccggctc atcacgaatt ccgggccgcc gcagttgaag
     7981 gccatgcgcg tgccatcggg cgccgtgaaa ggagcgccga cgcttgcgat atctttgtcc
     8041 cattcgccga ctgagaggca gaagccgcgt tcctcatagt ccttcactgc ctgatcgatg
     8101 ccgcgcttga tgcgcggcca gtcggattca tcctgaagac gcacctgctc gagcacctca
     8161 ttgcgctcat cctccggcgc cgccgccagg tacgctcgtc caatcgatgt cgtcgccagc
     8221 ggcaatcgga cgccgaccgc acgcatgacc gtaatcggcg cgctgctgcg cacccactcg
     8281 atgtagacga tcgaaagccg atcacgaacc ccgacggcca ccgatgcgcg cgcgtactcg
     8341 gcaagttcct gcatgaacgg ccgcgcgaca tcgcggatat ccagattcga cagcatcgaa
     8401 tagccaagcg agagaacgcc ggtgccaagg ctatatttcc cgagcggctc cgagtagcgc
     8461 agataaccga gcttcgcgag cgtgcccgcc aggcgcgcca cggtcgcttt aggcaggctc
     8521 gtgcgacgag ccagctccga cagccccaga tagcgctccc cggggccgaa gcaccgcagc
     8581 acctcaagtc ccctcgccag cgcggtcacg aactgccgat ccgtgccggc ttcttcgtcg
     8641 aaacgcgcca tgtcacgctg ctcatacggg gggaccgatg atcccgccac gctcttcgtc
     8701 ttcgacatgg ctgcactccg ctcctgcact cctgaaccct tccgtttgcc gaatcctgtc
     8761 atggtgaaaa tctccgtgtc aaacattttc taaaatcggt tcgcatagcg gaccattctg
     8821 gaacgatcgt gcaatttttg cacctcacac tggctgatgt gatgcaaacc ctagaaatat
     8881 tgcacgaata tggatcaatc agggttgatc gctccgtaga tgtaacggtg tttacattcc
     8941 gtgccggctc cgttactgtg cgtatcagtt caaaattcta tcctatcggt ccgcacagcg
     9001 gaccatcagg aagacaccct cggatcacct ttgacttcag gatgacttcc atgtccagag
     9061 cactttcaca ccttcgcatt ctcgatttgt ctcgtgtgct ggccggcccc tgggcgagcc
     9121 aaacgctggc ggatctgggc gccgaggtga tcaagatcga acgtcccggc acgggcgacg
     9181 acacg
//
LOCUS       DATASH010001083         5370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4141495,
            whole genome shotgun sequence.
ACCESSION   DATASH010001083 DATASH010000000
VERSION     DATASH010001083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5370
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4141495"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..781
                     /locus_tag="VHV99_28710"
     CDS             2..781
                     /locus_tag="VHV99_28710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX3639392.1"
                     /translation="MGFLFLYIPIVSLIVYSFNESQLVTVWTRFSTRWYAALLQDDEL
                     IAAAWLSLRVALLTAFASVFIGTWAGFVLARMGRFRGFTLYTGMINAPLVIPEVIQGI
                     SLLLLFIEMAKWLGWPAGRGIFTIWIGHVMLCISYVAIIVQSRVKELNPSLEEAALDL
                     GATPVKVFFFITLPLISQALVSGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVR
                     LGLNPEMNALATLFIAVVTVGVVVANYFMQRMERKRAVMAV"
     gene            complement(1489..2694)
                     /locus_tag="VHV99_28715"
     CDS             complement(1489..2694)
                     /locus_tag="VHV99_28715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3639393.1"
                     /translation="MRVNPQAFFCSLFVARLADQVLLFLVPLVVFQTTHQVSWSGLAF
                     FIETLPRYLVFPFFGALCDRISPLKLMRVSQTVRALVCFGGVLAYVLFGGIGWLIALS
                     ALCGVLTSQGLVAREVMLPQIFSTQQFQRVLAYSQLADQLGFVLGPILAAVLLGVWRW
                     EWVVGATGALFLLADAALLLWQRTSGFRAGDAPPAPGHWTMSLRIALRHVLVLPGLKK
                     VVLLAAAENLVIGVTLATSAAMVTGLHAQSNRYYAGLQTAGAVATVLILLSIARHAWP
                     AQTLGRVAFVAICAGGVMAGASAAPWGYALGFLLIVGFDKMFNVYIRSARQKIIPPED
                     YGKTTGVIILLNNLTQPLAGLLVGVFSTRAQTGVLIVALSAAMGCIGAAVSIGSALSR
                     RRRTLALGE"
     gene            complement(2945..3433)
                     /locus_tag="VHV99_28720"
     CDS             complement(2945..3433)
                     /locus_tag="VHV99_28720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010091408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3639394.1"
                     /translation="MSSPAVKPIPDGMHSLTPYLICANAAEAITFYAKAFNAVEQFRL
                     PGPGGKVMHACLKIGDSMLMLTDEWPEHNTFGPNALKGTPVTIHHYVEDVDASFKQAV
                     EAGASVTLPVTDMFWGDRYGQLKDPFGHSWSLATHKRDLSPEEIQQAMQSMQGMECGG
                     SQ"
     gene            complement(3741..5354)
                     /locus_tag="VHV99_28725"
     CDS             complement(3741..5354)
                     /locus_tag="VHV99_28725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX3639395.1"
                     /translation="MPQTSRAATLCVTLAAAATFAACSNPPPSHDGHASVDTVTLFPI
                     ANYDQNVDHWLEPDSPGYDQPFLSPADQQAHFRALYARYFGTGTSAPSPWNSAYVTMR
                     VYRQQGTDIAALQQRRIGKFDNTGKSGAALGYGENFRPHDKTWIDAIAQNMDLGQFTQ
                     EAVYKPERRAIATANLMVRELPTADPSFYDHRLAGEGYPFDNLQISAVRPGTPLYVLG
                     SSVDGAWRYVQTPDVQGWVRSDGVGLADAIFIDRWRAATAKSLGAVTVASAPVRDSRG
                     VFRFDAPAGTLLPLATNDATRPTAQAQAQATRTASGASDAPGTSASRTLLVPARDVDG
                     RAIIRTAQLSEAQIAPMPLAASPRHLAMLMKTLIGRPYGWGNSGLYNDCSSELQSIFA
                     AFGVWLPRHSSTQMSAGRMIDLSASTPAQRLDYLAQHGEPLRTLIYIGGHVMLYIGNT
                     TRNGVAVPVVYQDVWGLRPTDNSRRAVIGGSVILPLFEHIPEDATLQSLAATPTFQIS
                     ILGAPAGAAATPAAPAAPAAPPDDDNPAG"
BASE COUNT          940 a         1579 c         1751 g         1100 t
ORIGIN      
        1 gatcgggttt ctgtttctct acattccgat cgtgagcctg attgtgtatt cgttcaacga
       61 atcgcaactg gtcacggtgt ggacgcgctt ttccacccgc tggtatgcgg cgttgttgca
      121 ggacgatgag ttgatcgcgg ctgcgtggtt gtccctgcgg gtggcgttgt tgacggcgtt
      181 tgcttcggtg tttattggca cgtgggcggg gtttgtgttg gcccggatgg ggcggtttcg
      241 tgggtttacg ctctataccg ggatgatcaa tgcgccgttg gtgattcccg aagtgattca
      301 ggggatctcg ctgctgctgt tgttcatcga aatggccaag tggttggggt ggcccgctgg
      361 gcgcggcatt ttcacgatct ggatcgggca cgtgatgttg tgtatttctt atgtggcgat
      421 catcgtgcag tcgcgggtga aggagttgaa tccttctctt gaggaagcgg cgcttgatct
      481 tggggctacg ccggttaagg tgtttttctt tatcacgttg ccgctgattt cgcaggctct
      541 ggtttctggg tggttgttgt cgtttacttt gtctatcgac gatctggtgt tgtcggcgtt
      601 tctgtccggg cctgggtcga cgacgctgcc gctggttgtt ttttcgcgag tgcggcttgg
      661 gttgaatcct gagatgaatg cgcttgctac gctgtttatt gctgttgtta ctgttggggt
      721 tgtggttgct aattatttta tgcagcggat ggagaggaag agggctgtga tggcggtgta
      781 ggtttttttt gcctgtgcgg cgcttgttgt ctggttgcct gcggtggtgg gattttcttg
      841 atttgttttt cgccgatgtg gggcgttttg gctgtttgct gatggtgttc gcggctgctg
      901 gaattgtttg cctgcggcgg tgggtttggt tgtttgccta ccgggttggc ctttgtttga
      961 actgactttt tggcctttcc ttgatttgtt ggtggtctat tagcgttgcc cctgtgcggg
     1021 gcggcaccta cttttctttg cctgccgcaa agaaaagtag gcaaaagaaa gcggctcaca
     1081 ccgccagctc ataagcgggc cccccgcgca gttccggtag tggtgcatct ggaatctggg
     1141 ttctcgcaca ttccgcgtta gtgacacagc catcattctt ccggcggcgc tgcgcgcgcc
     1201 gaaaaggaat tcgaaaaccg atcgtctgag attcgcgctc acgcttcatt ccgcacaacg
     1261 gctcagcgtg tatcgcctcg atcgtcccag tcacgcaagc cattcagtca tcccgtcacc
     1321 cgattaccgg atcacccgat tacccgaggc aacgcggtca actcagtcgc tcctgtgcgt
     1381 tgcacacgcg ttgcgctgcg tacctcgcat ctccaggtct cgcggcggcc tgactgcgtc
     1441 gaccgtgcgc aatgtcaatt gcggctttcg gacagggcgc gcatggcgtc attctccaag
     1501 cgccagcgtg cgcctgcgcc gcgatagcgc cgagccgatc gataccgccg cgccaatgca
     1561 gcccatcgcc gccgagagcg ccacgatcag tacgccggtt tgggctcgtg tcgagaatac
     1621 gccaaccagc aatccggcga gtggttgcgt caggttgttc aatagaatta tcacccccgt
     1681 cgtcttgccg tagtcttcgg ggggaatgat tttctgccgc gcgctgcgga tgtagacgtt
     1741 gaacatcttg tcgaagccga caatcaacag aaagccgagc gcatagcccc aaggcgccgc
     1801 gctcgcaccg gccatgacac cccccgcaca gatcgcgacg aaggcgaccc ggccgagtgt
     1861 ctgcgccggc cacgcatgac gcgcgatcga caaaagaatc agtaccgtgg ccacggcgcc
     1921 agccgtttgc aggccagcgt agtagcggtt cgattgcgcg tgaagacctg tcaccatcgc
     1981 ggccgatgtc gcaagcgtca cgccaatcac gaggttctcg gcggcggcga gcagcacgac
     2041 ttttttcagg ccgggtaaaa ccagcacgtg gcgcagcgcg atgcgcagag acatcgtcca
     2101 gtgacccggt gcaggaggcg catcgccagc gcggaaacca ctggtgcgtt gccacagcag
     2161 tagcgccgca tcggcgagca gaaacagcgc gccagttgcg ccgaccaccc actcccaacg
     2221 ccacacgcca agcagtacgg cagccagtat cgggccaagg acgaagccca actggtcagc
     2281 cagctgcgaa taggcgagca cgcgctggaa ctgctgcgtg ctgaagattt gcggcaacat
     2341 cacttcgcgc gcgaccaaac cctggctcgt cagcactccg cacaacgccg acagcgcgat
     2401 caaccagcca atgccgccaa acaacacgta agccaacacg ccaccgaaac acaccagcgc
     2461 ccgcacggtc tgactcaccc gcatcagctt gagcggcgaa atacggtcgc acaacgcgcc
     2521 aaagaaagga aacaccagat aacgcggcag cgtttcaata aagaacgcga gacctgacca
     2581 cgatacctgg tgtgtcgtct gaaatacgac gagcggcacc aggaacagca agacctgatc
     2641 ggcgagacgc gcgacaaaca gcgagcagaa aaaagcctga gggttgactc gcaccgttca
     2701 cgtcccgccg tttatgggtc gagaccgatc ctatcaaaga tcgacgggtc acggcccagg
     2761 tcacggccag cacgctgccc ttcggtcggg gacccccgcc gcgtagtgca tcgtggtggc
     2821 gatgcgcgaa tcgttgagga cgcccgctta aaagcgggcg gcttctttcg cgttaccgtg
     2881 cgaaccacag gcagggcgcg atctgttgca tacgacctcc tggtaccggc atgggggagg
     2941 cggcttattg cgaaccgccg cactccatgc cttgcatact ctgcatggcc tgctgaatct
     3001 cctccggact cagatcgcgc ttgtgcgtgg caagcgacca gctatggccg aaggggtcct
     3061 tcaactggcc gtaacggtcg ccccagaaca tgtcggtaac cggcagggtg acgctcgcgc
     3121 ctgcctcgac agcctgcttg aaactggcgt cgacatcttc gacgtagtgg tgaatcgtca
     3181 ccggcgtgcc ttttaatgcg ttcgggccga atgtgttgtg ctcgggccac tcgtcggtca
     3241 gcatcagcat ggagtcgccg atcttcaggc aggcgtgcat cactttgccg ccgggtccgg
     3301 gcaagcggaa ctgctcgacg gcgttgaacg ccttggcgta gaacgtgatg gcttcggctg
     3361 cgttagcgca aatcagatac ggggtaagcg agtgcatccc gtccgggatc ggtttgacag
     3421 cggggctgga catgacggcg actccttgaa gtaacagttc ttgagttgaa aaaccggctg
     3481 cgtgagccag accttcaacg ttacgacgag tgcatcgcct ttggatcgac actccggaaa
     3541 aaatattttt aagcacgggc gtgctgtgag gggcagacgc gcagatggac gcgcgtgcag
     3601 gcgtggacgt ctgcgcgaga acgggcgtcg acgcgaccat ggccgccggc gcgcgacgct
     3661 gggacgccgg gacgccggga ctcagagact cacggactca cggactcacg gacgcagaaa
     3721 cgccaaaacg ccgcgcaagc tcaaccggcc ggattgtcgt cgtccggcgg cgcggccggc
     3781 gcggccgggg ccgccggcgt tgccgctgca ccggccggcg cgccgaggat gctgatctga
     3841 aacgtcgggg tcgccgcaag cgactgcaag gtcgcgtctt ccggaatgtg ttcgaacagc
     3901 ggcaggatca ccgaaccgcc gatcaccgcg cgccggctat tgtcggtcgg ccgcaagccc
     3961 cacacgtcct gatagacgac cggcaccgcg acgccgttgc gggtcgtgtt gccgatgtac
     4021 agcatcacat gtccgccgat gtaaatcagc gttcgcaacg gttcgccgtg ctgggcgagg
     4081 tagtcgagcc gttgcgccgg cgtggacgcg gacagatcga tcatccgtcc ggcgctcatc
     4141 tgcgtcgacg aatggcgcgg cagccataca ccgaatgccg cgaaaatgct ttgcagttcc
     4201 gacgaacagt cgttgtagag gccgctattg ccccagccat acgggcgccc gatcagcgtt
     4261 ttcatcagca tcgccagatg ccgcgggctg gcggcgagcg gcatcggggc aatctgtgcc
     4321 tcgctcagtt gggctgtgcg gatgatcgcc cggccgtcca catcgcgggc cgggacaaga
     4381 agcgtgcggg aggccgacgt gccgggggcg tcacttgctc cgcttgctgt tctagttgcc
     4441 tgtgcctgtg cctgtgccgt gggccgcgtg gcgtcgttcg tggcgagcgg cagcagcgtg
     4501 ccggcgggcg cgtcgaagcg gaacacgccg cggctgtcgc gcaccggcgc cgaggccacc
     4561 gtcaccgcgc ccagcgactt cgcggtggcg gcgcgccatc tgtcgatgaa gatggcgtcg
     4621 gccaggccga cgccgtcgct gcgcacccag ccttgtacgt cgggcgtctg cacgtagcgc
     4681 cacgcgccgt cgacgctgct gcccagcaca tacagcggcg taccaggacg taccgcggag
     4741 atctgcaggt tgtcgaacgg atagccttcg ccagccaggc gatgatcgta gaacgacggg
     4801 tcggcggtcg gcagttcacg caccatcagg ttggcggtgg cgatcgcgcg gcgttcgggc
     4861 ttgtacacgg cctcctgcgt gaactggccg aggtccatgt tttgcgcgat cgcgtcgatc
     4921 caggtcttgt cgtgcggccg gaaattctcg ccgtagccga gcgccgcgcc gctcttgccg
     4981 gtattgtcga atttgccgat gcggcgttgc tgcaacgccg caatgtccgt accttgctgg
     5041 cgatagacgc gcatcgtcac gtaagcggaa ttccacggcg acggtgcgct cgtaccggtg
     5101 ccgaaatagc gcgcgtagag ggctctgaag tgcgcctgct gatccgcggg gctcagaaac
     5161 ggctggtcgt agccggggct gtccggttcg agccagtggt cgacgttctg gtcgtaattg
     5221 gcgatcggga acagcgtgac ggtgtcgacg ctcgcgtggc catcgtgtga gggcggcgga
     5281 ttgctgcagg cggcgaaggt cgcggctgct gcgagggtga cgcataacgt ggcggcgcgg
     5341 ctggtctgcg gcaatcgggc ggcgaggcgt
//
LOCUS       DATASH010001084         5259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5135319,
            whole genome shotgun sequence.
ACCESSION   DATASH010001084 DATASH010000000
VERSION     DATASH010001084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5259
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5135319"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="VHV99_28730"
     CDS             complement(<1..888)
                     /locus_tag="VHV99_28730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX3639396.1"
                     /translation="MFFDELSNDEWALLAALVSDEPAVRLNRRGRPRAEPRIVANAVL
                     WIMTTGEPWSKLPGRYPSGPTCRRRFEEWQLNGTLLEMVRLLSHTGRTFAYVPQPGPP
                     VAAKPAAPVAETPSARDESLRGVSWKSPESWQAPGVSSSLSKWRSADPIADITRQLSG
                     LTNVPPVSRAPSAPAAPPASSSLAARPAMRADLQGMDEARAVGAAMHGLDSAITSARR
                     AHQLDDQRSPLTMSLAPRGTQIDDSRGYVIYVAAEQVPNVMYRAWAEIMKDGQRVERS
                     GLVGPRFAQANAAEQYALEW"
     gene            complement(1473..1769)
                     /locus_tag="VHV99_28735"
     CDS             complement(1473..1769)
                     /locus_tag="VHV99_28735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI-like protein"
                     /protein_id="HEX3639397.1"
                     /translation="MHYLLMYDLVPDYLERRGTYRDDHLKLAWAAVGRGELQLAGALT
                     EPVDTAVLLFEGDSPAAAEAFAKADPYVLAGLVTRWRVRPWMTVAGEGAANPVR"
     gene            2221..2517
                     /locus_tag="VHV99_28740"
     CDS             2221..2517
                     /locus_tag="VHV99_28740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsiF family protein"
                     /protein_id="HEX3639398.1"
                     /translation="MKIQSAIATLVLGAVLVSPAFAENSQQTKMADCNKQATGKKGDD
                     RAAFMKTCLSAKPAAAAPMSQQDKMKACNVQATGKKGDDRKAFMSTCLSSKPAN"
     gene            2803..3249
                     /locus_tag="VHV99_28745"
     CDS             2803..3249
                     /locus_tag="VHV99_28745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639399.1"
                     /translation="MVSKHKNRWLRRWLVVIVFWAVPVAIVAVSEIREEMAYNAVDLD
                     RALTTWNFTDAQRAAGAPAHCHGKPAEAQAAGCPADVLAANAPRQQDARNEYSVRRST
                     LMAYLWHAFVGYWIVPAVTLFVMGVLIGMIRRALRRPPVKSPANHG"
     gene            3609..4196
                     /locus_tag="VHV99_28750"
     CDS             3609..4196
                     /locus_tag="VHV99_28750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HEX3639400.1"
                     /translation="MQRRNFMLKTTAALAFGGLSLAGCTTTGSKHDTPSTDASKRQSI
                     DASVDGTMSRLFTTVKGSRELVSKARGVLVFPSVLQVGFVVGGQYGEGSLRVGGGTVG
                     YYSTISGSFGLQAGAQSKAIIFLFMTQDSLDKFRNADGWSVGADASVALVKMGANGAV
                     DTTTATAPVEVFVLTNTGLMADISLQGTKVTRLKI"
     gene            complement(4481..>5259)
                     /locus_tag="VHV99_28755"
     CDS             complement(4481..>5259)
                     /locus_tag="VHV99_28755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX3639401.1"
                     /translation="YSFSVFKRQDTPFVTTMHGRLDLPEQQPVFDTFNTAPVISISNA
                     QRHPLPQARWLTTVYHGLPEQLYTPQPVEQKYLAFLGRISPEKRVDTAIRIAGRCGMQ
                     IRIAAKVDAADCEYFEREIRPLLDLPYVEYIGEIADNQKAEFLSGAHALLFPIDWPEP
                     FGLVMIEAMACGTPVIAFNRGSVPEVLEDGVTGFIVEDEIGAVAAVNRLHKVPRTGVR
                     QRFEERFTSHRMAQQYVDAYQSVIRAQKRARFKVIDTSGS"
BASE COUNT         1019 a         1653 c         1574 g         1013 t
ORIGIN      
        1 ccattcgagc gcgtattgct ccgccgcgtt ggcttgcgca aagcgcgggc cgacgaggcc
       61 ggaacgctcg acgcgttggc cgtccttcat gatctccgcc cacgcgcgat acatgacgtt
      121 gggcacctgc tccgccgcaa catagatcac gtagccgcgc gagtcgtcga tctgcgtgcc
      181 acggggcgcg agactcattg tcagcgggct gcgctgatca tcgagctgat gcgcgcggcg
      241 tgccgacgtg atcgccgaat ccagaccgtg catggccgcg cccaccgccc gtgcttcgtc
      301 catgccttgc agatcggcac gcatggcggg ccgtgctgcg agcgacgaag acgccggggg
      361 tgccgccggt gccgagggtg cgcgcgacac cggcggcaca ttcgtcagcc cggacaactg
      421 gcgcgtgata tcggcgatcg gatcagccga acgccacttg ctcaggctgc tcgacacgcc
      481 gggcgcctgc cacgactcag ggctcttcca cgacacgcca cgcagacttt cgtcacgcgc
      541 gcttggggtt tctgctaccg gcgccgcggg tttggccgcg acgggcggcc ccggttgggg
      601 gacgtacgcg aaggtacgcc cggtgtgcga caacagccga accatctcga ggagggtgcc
      661 gttcaactgc cattcttcga aacggcgccg gcatgtggga cctgacggat aacggcctgg
      721 cagcttcgac cagggttcac cggttgtcat aatccacagg accgcattcg ccacgatgcg
      781 cggttcggcg cggggccggc cgcgtcggtt gagacggacg gctggctcat cagagacaag
      841 cgctgccagc agcgcccatt cgtcattgct tagctcatcg aaaaacattg ggttttctcc
      901 acgcagcctg gcccggccgc ggctttggac agccacagat tcaactttca cgatccgtgt
      961 gcagcccgcg gttgcacaaa tatgttttgt acgttctggt tgttgggcaa tgtcgcactg
     1021 ggttagtgcg gtttgcccct attgcgaagc acaaaacgtg gtctcactcg tgcatgggag
     1081 aatttgtgca cgaagcatac catcaccagg gtttgcgtga atatcactga gacaagtgtt
     1141 acggatggaa acaaacttgt ctttttttct gcgtcatttc acgcattgct gtaacagaac
     1201 cgtagtcggt ccggacggcg aagcatgcta ttgcacggca caatgacacc gtaacagccg
     1261 ggaatggctt ttatgaaatc agcgcatgag atgcaacgca ttttgccggc tttttaaggt
     1321 attttcgcgg agccgcgcag tgcccgagcg cactgggtgg acagcgcccg gctaaggcca
     1381 tgacgcaatg gtcgcgcaag cctgcacagc gcctcgcgta tgctattcgc attcctttcg
     1441 atgccgcgca ggtgtggcct atcggctggc ttctagcgca cagggttcgc cgcgccctca
     1501 cccgccactg tcatccatgg gcgtacgcgc cagcgcgtca cgagccccgc gagcacatac
     1561 ggatcggcct tggcaaaggc ttcggcggca gcgggagagt cgccttcgaa aagcagcacg
     1621 gcggtatcca ccggttcggt caacgccccg gccagctgca attcaccgcg ccccaccgcc
     1681 gcccaggcga gtttcagatg gtcgtcgcga taggtgccgc gccgctcgag atagtccggc
     1741 acgaggtcgt acatcaacag atagtgcatg gcgggtctcc tttggcctgg gtgaaaaagt
     1801 tgacgaacag ggtcggacac agcgattgtg gtcaatatta cccttatgat cgtcaacggg
     1861 attttctgcc gatttactat tcatttgcct ctgaaacaat gcggatcgaa tgcttcatat
     1921 tttgacatcc gaatcgaatt gaggaagcgc taattcattt acatgcgttc ccgccaatta
     1981 gtgcggattg cataacatat tcttacaccg ctacgattca gctgtttcaa tcaccgctct
     2041 attgccgcgc cccgcgcgta ggggaaaacc agcgtgggcg tttgtcaacc ggaatgcgct
     2101 tctgtccgtt ctggcagaca gcgcttgcca taatggacgc gcgctgcggg aagcctctac
     2161 gccgtcaaag cccgcgcaca cgaatgacca aaaacgtttt caccaccgat ggagtgtcac
     2221 atgaaaatcc aatccgctat cgctacgctg gtgctgggcg cggtcctcgt ttcgccggcg
     2281 ttcgcggaga acagccagca aacgaaaatg gccgactgta acaagcaggc caccggcaag
     2341 aaaggcgacg accgcgcagc cttcatgaaa acctgcctgt cggccaagcc ggccgctgcc
     2401 gcgccaatga gccagcagga caagatgaag gcgtgcaatg tccaggctac cggcaagaaa
     2461 ggcgatgacc gcaaggcctt catgagcacc tgcctgtcgt cgaagcccgc taactaaact
     2521 aaacgcggga ccgtcggcgc ggcccacacg ccgcgcaacc agcaacgttt tgctcgaaca
     2581 gggccgcgcc attgtccggc gcggctgctg cgctgcttgt tgcctgacgc ctgctttccg
     2641 ctgtaacccc tcactctcgc gccccccttc ccctcagagt ccatccgccc gagcgcctcg
     2701 tttttcttct atgatggctg cggagacggc ccaccccata cacaacatta aacgggccgc
     2761 catcttgtcg ttggtgctgc agagcatcca tcggaggcaa ggatggtatc gaagcataaa
     2821 aaccgctggt tgaggcggtg gctggtcgtc atcgtattct gggccgtgcc cgtggcgatc
     2881 gttgcggtca gcgagatccg ggaggaaatg gcgtacaacg ccgtcgacct cgaccgggcg
     2941 ctgaccacct ggaatttcac cgacgcccaa cgtgcggccg gcgcgcccgc gcactgccat
     3001 ggcaagcccg ctgaggcgca agccgccggc tgccctgcgg atgtgctggc cgccaatgcg
     3061 ccgcgccagc aggatgcccg caatgaatac agcgtgcgcc gctccacgct catggcttac
     3121 ctctggcacg cattcgtcgg ttactggatc gtgccggctg tcacgctgtt cgtaatgggc
     3181 gtgttgatcg gcatgattcg ccgtgcattg cggcgcccgc cggtcaagag tccggcgaac
     3241 catggctgag gatgcgatgc ggcgtcgagg caacactttc agttcgattt caatgcgaat
     3301 cgcgacggca aaaaatcggt aaaaacgggc acttgatacg ctttcggccg cgccgccgac
     3361 atgctgagcc atgcgaggca ctgctcgtcg ggagcccatt cgaacgcgct gtccgcgctt
     3421 ctttcaacac gcctctgatg ccggtcaaat ttacatcaga gcaccttaca actggacgca
     3481 aacccccgcc acacaaggct tttcgcgaaa cgacgaagta cgcctttagg cttgcgtgtg
     3541 atataactca aaggcaaccc ggcaacacca cacgaggttg cgcccaggaa ccatgatgga
     3601 gaaaaacgat gcaaagacga aacttcatgt tgaaaactac cgctgcgctc gctttcggag
     3661 gcctttcact cgctggttgc acgacgaccg gcagcaagca cgatacgccg tccactgatg
     3721 catccaaacg ccagtcgatc gacgccagcg tcgacggcac catgtcgcgg ctgttcacaa
     3781 ccgtgaaggg ttcgcgcgaa ctcgtctcca aagcgcgcgg tgtactggtg ttcccgtcgg
     3841 ttctgcaagt gggcttcgtc gtcggcggcc agtatggcga aggttcgctg cgcgtgggcg
     3901 gcggcacggt cggttactac agcacgattt ccggttcgtt cggtctgcag gcgggcgcgc
     3961 agtcgaaggc catcatcttc ctgttcatga cgcaggactc gctcgacaaa ttccgcaatg
     4021 cagacggctg gtcggtaggc gccgacgcct ccgtcgcgct ggtgaagatg ggcgcgaacg
     4081 gcgcagtcga cacgaccacg gcgacggccc cggtcgaagt gttcgttttg accaataccg
     4141 gcctgatggc cgatatctcg ctgcagggca ccaaggttac gcgcctgaag atctaggcgg
     4201 gcgcccggtt acggcctgcg cctgacccgc cggcttcgca cgcggtgcta gccgggccag
     4261 acgcaggcca atccgtcaga cggccgccgg tgctgcatgg gttcggtgcc gctgctgtag
     4321 tttcaggcag ccgccgcttc ccgcagccgc gtggttgccc tggcaggtct gacgtggtcc
     4381 ctcaagactt ttagcctcgt tgcggcggca ataaaaaacg gcgatgcgca ctgcaccgcc
     4441 gttctttttt gcactggcgc ccccgtcaac gaagcgccgc tcaacttccc gacgtatcga
     4501 tcaccttgaa gcgtgcacgc ttctgagcgc gaatcaccga ttgatacgcg tccacatact
     4561 gctgcgccat ccggtgcgag gtgaagcgct cctcgaagcg ttgccgcacg cctgtgcgcg
     4621 gcaccttatg caggcgattg accgccgcca ccgcaccgat ctcgtcttcg acgataaagc
     4681 ctgtcacgcc gtcttccagc acctccggca ccgaaccgcg gttgaacgcg atcacgggcg
     4741 tgccgcacgc catcgcttcg atcatcacga ggccgaacgg ctccggccag tcgatcggga
     4801 aaagcaacgc atgggcgccc gagagaaact cggccttctg gttatccgcg atttcgccga
     4861 tgtactcgac atacggcaag tccagcaacg gccggatctc gcgttcgaag tactcacagt
     4921 cggcggcgtc cactttcgcg gcgatgcgga tctgcatgcc gcatcggccg gcaatccgga
     4981 tcgccgtgtc cactcgcttc tcgggcgaga tacggccgag aaacgcgagg tacttctgct
     5041 cgacgggctg cggcgtgtag agctgttccg gcagaccgtg atagacggtg gtgagccacc
     5101 tggcctgcgg caacggatga cgctgcgcat ttgaaatcga aatcaccggc gcggtgttga
     5161 aggtgtcgaa caccggctgt tgctccggca agtcgaggcg gccatgcatc gttgtgacga
     5221 aaggcgtgtc ctgacgcttg aagaccgaga acgagtagt
//
LOCUS       DATASH010001085         3625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4541145,
            whole genome shotgun sequence.
ACCESSION   DATASH010001085 DATASH010000000
VERSION     DATASH010001085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4541145"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            565..990
                     /locus_tag="VHV99_28760"
                     /pseudo
     CDS             565..990
                     /locus_tag="VHV99_28760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018423374.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 domain-containing protein"
     gene            1074..3452
                     /locus_tag="VHV99_28765"
     CDS             1074..3452
                     /locus_tag="VHV99_28765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX3639402.1"
                     /translation="MFNFALRRLPFCARRSAWRIATGRPSLSNFQAYWPALVPAFAPA
                     FIATSAHADTAPADATLPAVSVTANAVTGPRPVDPDLPASVETVTRDQFDNWNVVNTE
                     DVLKYMPNLAVRKRFIGDLNSIIAVRGTSNTQSARGLVYADGLLLSNLLGNSYAFPPR
                     WSMVFPDEIQQVDVIYGPFSALYPGNSLGATVLISTRMPNKFEADADVKAFTQHFSLF
                     GVNQNFNGSEASASLGDRIGKLSYFLGVNHLENTSQPLQFATLARSSTPAKAGDTPVT
                     GAYFYNNQTNTPTAVLGVNGEGIEHTVQDQFKLKMQYDFTPILQAAFTLGYWHQTYNS
                     ETSSFLRDANGNPVYSGKVAIGGYEYTIPAAALAPGLGHSENWLYGLTLKTRNATGWN
                     GEAIASYYDIGNSVARTANSGAPGNGPGTVIFGDGAGWKTLDLRTTYTPVTPQAGWTN
                     HALSFGYHYDNYFLDNESYNTLNWRDGAATSFANAFAGKTQTQALYAQDAWRFLPRWK
                     LVYGVRYEDWQAYGGSQSLPGTAVPYSDVNQHHFSPKASLSFDVTDDLSLRASIGRAY
                     RFPTVGEMFQGQINGSSIINNNPNLKPEDDLSKELTAEWAHWNGVFRFSLFQDDVKNT
                     IFSQTDTTVIPNVTNFQNIGKVRSRGVETSYSGQDVFVRGLDLLASVAYTQSRIIANA
                     QNPATVGKYFYRIPLWRANLAATYHIDERAAMTLAARYSGRQYNTLTNTDTNPDVFGG
                     TSSYTVADAKFTFKPTRLSEVGIGVDNLFDARYFVYHPYPGRTFYVEAKLRM"
BASE COUNT          689 a         1199 c         1031 g          706 t
ORIGIN      
        1 tcactctgcg tgacggcgca gaccggaaaa gccccggagg cgggtatcgc taccccatga
       61 aacccgcgag cacgcgcgta cacatgacga ccctcagaaa atgctatatc acggcacttt
      121 gcctactaat cgatgtatac ccttaacgag tcggactcgt taatcccggc ggtgcagtga
      181 agctttttgt aatcccactt agcggttttt tttcgccatg caatggcggc ctgactcgat
      241 tcgatggcgg gttcagcggg tcaagtgagg aatccaaagg tattcggccc gtgtggcttg
      301 agtgggttca catccgcttt ccgccatcgt cggcctgttc gttgcgagtc acgagggctg
      361 caagatcatt cttttagcgc cgcggagttc ggtagcctag ccggaggaaa aacgctccac
      421 acacgcctgc ctcacacgct cgaccgtgac gggcgcccgg aaacaagccg gttgcaggct
      481 tctaatccaa cgctataaac aggatcacac gcgaaaagag cgcgaggcaa tgctagaatc
      541 gccggcgtct ttacgctcat caccatgact ccacgctccc acaaccgcat gaccgcatgg
      601 cttggcctga tcgccatgtg gctcgtcgtg ttcgcgccga tcgtgagtca gatgctggta
      661 tcgaaccgcg cgcacgagcc tttcgcggcg ctcggttcgg cgctgcaacc ggccagccag
      721 agcctggtgg gccggagccc ggtgagccgg gtaatccagc cgacctagcc cagcccggcg
      781 cccgtccatc tcagccacga cgacgccttc ggcgcatgcg gctactgcca tctgctccaa
      841 catcacgtcg ccatgccaag cgtcgcgact gccgaaccgt cggccgcact cgcgctggcc
      901 ggcaccgcgc cgtccgccct ctccacccgc ttcacgccgc tcggcgcgtt cccgtcggga
      961 cgcccaagag acccgcccgc cgtttcctga tcgctgttgt tcctgatcgc gcatctcgtc
     1021 gcacccgttg agggggcgcg cgatcgcctg ttcaatgcat caaggaaacg ttcatgttca
     1081 acttcgccct gcgaaggctg cccttttgcg cccgccgtag cgcctggcgc attgccaccg
     1141 gccggccttc gctgtcgaat tttcaggcgt attggcccgc gctggttccc gcgtttgcgc
     1201 cggccttcat tgcgacgtcg gcccacgcgg ataccgcgcc agccgatgcc acgctgcccg
     1261 ctgtcagcgt caccgccaat gccgtcaccg gcccgcgccc cgtcgatcct gacctgccgg
     1321 cctcggtcga aaccgtgacg cgcgaccagt tcgacaactg gaacgtcgtc aacaccgaag
     1381 acgtgttgaa gtacatgccg aacctcgccg tgcgcaagcg ctttatcggc gatctgaatt
     1441 ccatcatcgc cgtgcgcggc accagcaaca cgcagagtgc ccgcggcctc gtctatgcgg
     1501 acggtctgct gctcagcaat ctgctcggca acagctacgc gtttccacca cgctggtcga
     1561 tggtgttccc cgacgaaatc cagcaggtcg acgtgatcta cggaccgttt tccgcgctct
     1621 atccaggcaa ttccctgggc gccacggtcc tcatcagcac gcgcatgccg aacaaattcg
     1681 aagccgacgc cgacgtcaaa gccttcaccc agcacttcag cctgttcggt gtgaatcaga
     1741 atttcaacgg cagcgaagcg agcgcgtcgc ttggcgaccg gatcgggaag ctctcgtatt
     1801 ttctcggcgt gaaccacctg gagaacacga gccagccgtt gcaattcgcc acgctcgcca
     1861 ggtccagcac gcccgcgaaa gccggtgaca cgccggttac cggcgcgtac ttctacaaca
     1921 accagaccaa tacgccgacc gcggtgctcg gcgtgaacgg cgaagggatc gaacataccg
     1981 tgcaggacca gttcaagctg aagatgcagt acgacttcac gccgatactg caggccgcct
     2041 tcacactggg ctactggcac cagacctata acagcgagac gtcgagcttc ctgcgcgacg
     2101 cgaacggcaa ccccgtctac agcggcaagg tcgcgatcgg cggctacgag tacacgattc
     2161 cggcggccgc ccttgccccg ggtctcggcc acagcgagaa ctggctctac gggctcacgc
     2221 tgaagacccg caacgccacc ggctggaacg gcgaagccat cgcttcgtac tacgacatcg
     2281 gcaatagcgt ggcgcgtacc gcgaattcag gtgcgccggg caacggtccc ggcacggtga
     2341 ttttcggcga cggcgccggc tggaaaacgc tcgatctgcg caccacgtac acccccgtga
     2401 cgccgcaagc gggatggacg aaccatgcgc tgagcttcgg ctatcactac gacaactact
     2461 tcctcgacaa cgagagctac aacacgctga actggcgtga cggcgcggcc acctcgtttg
     2521 ccaatgcgtt cgccggcaag acccagaccc aggcgctgta cgcgcaggat gcatggcgtt
     2581 tcctgccgcg ctggaaactg gtctacggcg tacggtatga ggactggcaa gcctatggcg
     2641 gttcccagtc gctgcccggc acggccgttc cgtacagcga cgtgaatcag catcacttct
     2701 cgccgaaagc ctcactgtcg ttcgatgtca ccgacgatct gagcttgcgc gcatccattg
     2761 gccgcgccta tcggtttccg actgtgggtg agatgttcca ggggcaaatc aacggctcgt
     2821 cgatcatcaa caacaatccg aacctgaagc ccgaagacga tctctccaaa gagctcaccg
     2881 ccgaatgggc gcactggaac ggcgtgttcc gcttctcgct gtttcaggac gacgtgaaga
     2941 acacgatctt cagtcagacc gatacgaccg tgattccgaa tgtgacgaac ttccagaaca
     3001 tcggcaaggt gcgctcgcgg ggcgtcgaaa cgagctactc gggacaggat gttttcgtgc
     3061 gcggcctgga tctgctggcg agcgtggcat acacgcagtc caggatcatc gcgaacgcgc
     3121 agaacccggc gactgtcggc aagtacttct accggattcc actgtggcgc gccaacctcg
     3181 ccgcgacgta tcacatcgac gaacgcgcgg cgatgacgct cgccgcgcgc tactccggcc
     3241 gccaatacaa cacgctcacc aataccgata cgaatcccga tgtattcggc ggcaccagtt
     3301 cgtacaccgt ggcggacgcg aagttcacgt tcaagccgac caggctgagt gaagtcggca
     3361 ttggcgtgga taacctgttc gatgcgcgtt acttcgtcta tcacccgtat ccgggccgca
     3421 cgttctatgt cgaagcgaag ctgcgcatgt gaacacgcgg gttgtttatg cggtttgtgt
     3481 ttgccctgtg tttgccctgt gtttgccctg tgtttgccct gtgtttgcct tgtgtttgcc
     3541 ttgtgcttgt cctgtgcttg tcctgtgctt gccgatgtgc ttgccgatgt gcttgccgat
     3601 gtgcttgccg atgtgcttgc cgatg
//
LOCUS       DATASH010001086         5055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1377862,
            whole genome shotgun sequence.
ACCESSION   DATASH010001086 DATASH010000000
VERSION     DATASH010001086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5055
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1377862"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            312..695
                     /locus_tag="VHV99_28770"
     CDS             312..695
                     /locus_tag="VHV99_28770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639403.1"
                     /translation="MKILHIATCSMLFAWCCAATAADDTPCATLVGTANPATSHSFQV
                     RDGEPVDLVSGAKTVHGKLLVFGDGGVYRAYWQPENSSEKYVLANAGENSVRLVSTPP
                     QGTPAQNGAPGTTLAPQRVLSCPAL"
     gene            962..2926
                     /locus_tag="VHV99_28775"
     CDS             962..2926
                     /locus_tag="VHV99_28775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX3639404.1"
                     /translation="MVRLGRLREALSVAEGDPELVRSQLQAFGRQIPLLYFILMVNTM
                     AVAVTHLRSAPAWLSIYFPAALCTLCVIRCVRWWRVRHRALTHDKAVPELRKLIWLAG
                     IFGTAFCSWSLLLFPYGSAYEQAQVAFYMATTLVGCVFCLMHLRAAALLLLSIVILSF
                     ATFLVFTHSPVFIAMAIDMTLVGAALAFIIELYYRTFAGVVHAQRELQMSQRNTQRLS
                     DDNFRLASIDSLTDLPNRRSFFASLRALSEQAPGTGGFNVGLIDLDGFKQVNDIYGHA
                     SGDLVLQEVGVRLLALAEPGIFFARLGGDEFGVLVQHKLANETLVELGGRICESLCQP
                     YRVAGNVAELTGTIGWAAFPEAGTTVQQVFERADAALYAGKESRRGTPVIFSTEHETR
                     IRRSSLVAQELRHADLESELFLEFQPIHDVLTQRIIGMEALGRWHNARLGAVQPEEFI
                     RIAERTDLILRVTEVLLRKALAEVSRWPSDLYLSFNLSAIDISTSPRARRLVDIVSGS
                     GVPAHHVNFEITETAVTRDFEQAHCSLTMIKQAGCRVSLDDFGTGYSSLSYVHRLPFD
                     TIKIDRSFMTDVDCSSASTKIVKSVLDLCRNLGLECIVEGLETSSQADVVKALGARAV
                     QGYLFGRPMRASAVSTYLQTVPGERHVVQA"
     gene            complement(3020..3748)
                     /locus_tag="VHV99_28780"
     CDS             complement(3020..3748)
                     /locus_tag="VHV99_28780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HEX3639405.1"
                     /translation="MTDVDPSAQTLKPARLLAESPSRFPGESAAYRSARNDLLAEEIE
                     LRRHIERVAAQRRALPPGGLVPQDYRFESEAGPVTLAEMFGAHDTLVTYNWMFGPQRA
                     RPCPMCTSLLSAYDGEMPDILQRVAFAVIGRSPIEKLVAFKKERGWRHLRLYSSGGNT
                     FNRDYAAEDPDGDDHPAFNVFTRSGGTVRHFWAEEMGPSTADPGQDPRGAPDVMPIWT
                     VLDMTPGGRGADWYPKLEYEATPG"
     gene            4159..>5055
                     /locus_tag="VHV99_28785"
     CDS             4159..>5055
                     /locus_tag="VHV99_28785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX3639406.1"
                     /translation="MPLRLPPLPALRFFEAAGRHQSFKLAAAELNVTPSAISHGIVGL
                     EQSIGVELFVREPRGISLTAAGADYLSYISEAFSLIAIGTQRLPNHRANRPIALSCAP
                     TLASRWLLPRLAAFRTRWPDANVTVDTSHRQVGFPVDGFDFAIRLSRAPVAGTAWTRL
                     FGEHLVPVCSPAYLESLRDAHGNLDLRRATFIHVNSASEDWQAWLDGVAIDGVAIDGV
                     TVDGVAIDGIDLNDGLRVDTIQLAFEAASMGLGVALGRRPLVDRDLANGALVEACSQT
                     VASSTAYWLVCAQNADSAEHRPE"
BASE COUNT          917 a         1628 c         1535 g          975 t
ORIGIN      
        1 tgttgcgccg ggaccgacac actaacgcat aggcgcgtat tcgcgcaaac cttgggtgta
       61 agccttgtat cacccgccca gcggcaattt gacgtttttt tgtgccaaag tgccataaga
      121 atcgcgcgct tacgcgtttc ccccgcctta cggcgctgcg cggcgtatca caatccgttg
      181 caatttcagt gtgttacggg cggcaaaccc gtttcaggca ggctttttta ctatgttatg
      241 gtggatcgca aaattcgtaa ttcacctttg cgccgcgctc aggaattaaa gaattcacgg
      301 aacataaaac aatgaaaatt ctccatattg caacatgcag catgctcttc gcgtggtgct
      361 gtgccgccac ggcagcggac gacacaccct gcgcgacgct cgtcggcacg gcgaacccgg
      421 ccacttcgca cagcttccag gtgcgcgacg gcgaaccggt cgacctcgtc agcggcgcga
      481 aaaccgtgca cggaaagctg ctggttttcg gcgatggcgg cgtctatcgc gcttattggc
      541 aaccggaaaa cagctcggaa aaatatgtgc tggcgaatgc cggcgagaat agcgtccggc
      601 ttgtttcgac acctccgcaa ggcacgccgg ctcaaaacgg cgcaccggga acgaccctcg
      661 caccgcaaag ggtgctgtcg tgtccagcgc tgtagcgcgg caacgtccgc gggacgctgg
      721 cgagcccacg caggactatc tccacagcca agtccgaaga agccatacgc gcctccacgt
      781 tcctgacatc acaccgcgcg aagtccggcg cggtataaat gacctgccga caatccctta
      841 aagggcatat ttaccgctca cccatcgacg cgcgccgtat tttcatcgaa atcgcctcaa
      901 ggtctcaccg ccgctgccga tataaagctg cgcgtacagc gcaaacgtta cagggacggg
      961 gatggttcgg ttaggcagat tgagggaagc gctgtcggtc gcggaaggcg acccagagct
     1021 cgtgcgttcg caactccagg cattcggccg gcagatcccg ctgctttatt tcatcctgat
     1081 ggtcaacacg atggccgtgg ctgtcacgca tctaaggtcg gcgcccgcat ggttgtccat
     1141 ctacttcccc gccgcgctct gcacgctgtg cgtcatccgc tgcgtgcgct ggtggcgcgt
     1201 gcggcaccgt gccctgacac acgataaggc cgtgcccgaa ctgcgcaagc tgatctggct
     1261 cgcgggcatc ttcggtaccg ccttctgcag ttggtcactc ttgctgtttc cgtacgggag
     1321 cgcctacgag caggctcagg tcgcgtttta catggcgacg acgttggtcg gctgcgtgtt
     1381 ctgcctcatg catctgcgag cggccgccct gcttctgttg tcaatcgtca tcctaagctt
     1441 cgcgacgttt ctggttttca cacactcgcc tgttttcatc gcgatggcca tcgacatgac
     1501 gctggtgggt gccgcactcg ccttcatcat cgaactgtat taccggactt tcgccggcgt
     1561 cgtgcatgcg cagcgcgaat tgcagatgag ccagcggaac acgcaacgac tcagcgacga
     1621 caacttccgg ctcgcgagca tcgacagtct gaccgacctg cccaaccggc gcagcttctt
     1681 cgcctcgctg cgcgcgctat cggaacaggc gcccggtacc ggcggcttca atgtcgggtt
     1741 gatcgatctc gacggcttca agcaggtcaa cgatatctac ggccatgcaa gcggcgatct
     1801 cgtgctgcag gaagtcggcg tgcggctgct ggcgctcgcc gagcccggca ttttcttcgc
     1861 acgcctgggc ggcgacgaat tcggcgtact ggtccagcac aaactggcca acgaaacgct
     1921 cgtcgagttg ggtgggcgca tttgcgaatc gctttgccag ccctatcggg tggccggcaa
     1981 tgtcgccgaa ctgacaggca cgatcggctg ggcagcgttt ccggaagccg gcacgaccgt
     2041 tcagcaagtg ttcgaacgcg cggacgccgc cttgtacgcc ggcaaggaaa gccgccgcgg
     2101 cacgccggtg atcttctcga ccgaacatga gacacggatc cggcgctcga gcctcgttgc
     2161 ccaggagctt cgccacgcgg acctggagtc cgaactgttt ctcgagtttc agccgattca
     2221 cgacgtgctc acacaacgca tcatcggcat ggaggcactt gggcgatggc acaacgcacg
     2281 gctgggcgca gtccagccgg aggaattcat ccggattgcc gagcgtaccg atctgattct
     2341 gcgtgtcacc gaagtgctgc tgcgcaaagc gctcgccgaa gtctcacgct ggccgtccga
     2401 tctctatctg tcgttcaatc tctccgcgat cgatatttcg acttcgccgc gcgctcgccg
     2461 cctggtcgat atcgtatcgg gcagcggcgt gcccgcgcat cacgtcaact tcgagattac
     2521 ggaaactgcg gtcacgcgcg atttcgagca ggcccactgc tcgctgacga tgatcaagca
     2581 ggccggctgc cgcgtctcac tcgacgattt cggcaccggt tattcgagcc tcagctacgt
     2641 gcatcgcctg cccttcgata cgatcaagat cgaccgcagc ttcatgaccg acgtggattg
     2701 tagtagcgcg tcgacgaaga tcgtcaagtc ggtgctcgat ctgtgcagaa acctcggact
     2761 cgaatgcatc gtggaaggac tcgaaacatc atcgcaagcc gacgtcgtca aggcgctggg
     2821 cgctcgggcg gtacagggct atctgttcgg ccggccgatg cgggcgagcg cggtctcgac
     2881 ctacttgcaa acggtgcccg gcgaacggca cgtcgtccag gcgtaggcgc tctcgtctca
     2941 tgcctctcac ggcacacgaa tcagcgcggc aggtccagcg cagccaggca tgaaacacac
     3001 gcgtgacgcg agtgcatggc taacccggcg tcgcctcgta ttccagcttc gggtaccagt
     3061 cggcgcctcg gccgccgggt gtcatgtcga ggaccgtcca gatcggcatc acatcaggcg
     3121 cgccgcgcgg gtcctggccc gggtccgctg tcgacggccc catttcctcg gcccagaagt
     3181 gccgcaccgt gccgcctgag cgtgtgaaca cgttgaaggc gggatgatcg tcgccgtccg
     3241 gatcttcggc ggcgtagtcg cggttgaaag tgttgccacc cgacgaatac agccgcagat
     3301 gccgccagcc gcgctccttc ttgaacgcca cgagcttctc gatcggcgag cggccgatta
     3361 ccgcaaatgc cacgcgctgc aatatgtcgg gcatttcgcc gtcgtaggcg ctcaaaagcg
     3421 aggtgcacat cggacagggc cgcgcgcgct gcgggccgaa catccagttg taggtgacga
     3481 gggtgtcatg cgcgccgaac atctccgcaa gcgtcaccgg gccggcttcg ctctcgaagc
     3541 gatagtcctg cgggacgagg ccgccgggcg gcagcgcacg gcgttgagca gccacacgtt
     3601 cgatatggcg gcgcaattcg atttcctcag ccagcagatc gttgcgcgcg ctacgatatg
     3661 ccgcgctctc gccgggaaaa cggctcgggg attcggccag cagccgggcc ggtttcagcg
     3721 tttgtgcgga tgggtctacg tcagtcatca gccacctcct gttgcgggaa acgataaagg
     3781 gcgcgatagc agcgtggtgc ggcgcaaccg gttcgcgagc gcttttttgc ccctatacag
     3841 ccacgacgaa cagcgtgacg gtaaatcgac aacgccggtg gatttttttg aatcgtccgc
     3901 aacgccattc gagcgcgtcc ttttgatacg cgctcgacgt gccgatggcc cgcggacgac
     3961 acgcgcacga gacggcgttg tcccgcgtac gacacgcgga cgtcccgtga gcgaaatgcg
     4021 tacgagccgc ggcgccatgc cgacaccgac accgagagaa acctcattgc aaggtatcct
     4081 tccccgaaac agcacacgcg catcggcgcg ctatgacacc gtcacggcgc cccgctgcgc
     4141 cgcctctggg gatgcaccat gccgcttcgt ctgccacccc tgcccgcgct gcgcttcttc
     4201 gaggccgccg gcaggcatca gagtttcaag ctcgcagccg ccgaactgaa cgtcacgccg
     4261 agtgcgatca gtcacggcat tgtcggtctt gagcagtcga tcggcgtcga actgttcgta
     4321 cgcgagccgc gtggaatttc gctcacggca gcgggtgccg actatctgtc gtatatatcc
     4381 gaagcgtttt cgctgatcgc gatcggcaca caacgcttgc cgaatcaccg cgcgaatcgc
     4441 ccgatcgcgc tgagttgcgc gcccacgctc gcctcgcgct ggctgctgcc gcgcctcgcc
     4501 gcttttcgca cgcgctggcc ggatgccaac gtcaccgtcg acacgtcgca tcgtcaggtc
     4561 ggctttcccg tcgacggttt cgatttcgcg attcgcctga gccgcgcgcc ggtcgccggg
     4621 actgcatgga cgcgtctgtt cggcgaacat ctggtgccgg tgtgcagccc tgcttacctg
     4681 gagagtctgc gcgacgcgca cggcaacctc gatctgcgtc gcgcgacgtt cattcatgtg
     4741 aactcggcga gcgaggactg gcaggcgtgg ttggacgggg tcgccatcga cggggtcgcc
     4801 atcgacgggg tcaccgtaga cggagtcgcc atcgacggta tcgacttgaa cgatggcttg
     4861 cgtgtcgaca cgattcagct agcgttcgaa gccgcaagca tgggcctcgg cgtcgcactc
     4921 ggcagacggc cgctggtgga ccgcgatctc gccaacggcg cgcttgtcga agcctgctcg
     4981 cagacggtcg cctcctccac ggcctattgg ctggtgtgcg cgcaaaacgc ggatagcgcg
     5041 gagcatcgtc cggag
//
LOCUS       DATASH010001087         4238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6515088,
            whole genome shotgun sequence.
ACCESSION   DATASH010001087 DATASH010000000
VERSION     DATASH010001087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6515088"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VHV99_28790"
     CDS             <1..319
                     /locus_tag="VHV99_28790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HEX3639407.1"
                     /translation="GNTKVMRQALECTHKGWGQSFIIGVAAAGEEISTRPFQLVTGRE
                     WKGSAFGGARGRTDVPKIVDWYMEGKINIDDLITHRLPLERINEGFDLMKKGESIRSV
                     VLY"
     gene            465..1313
                     /gene="fghA"
                     /locus_tag="VHV99_28795"
     CDS             465..1313
                     /gene="fghA"
                     /locus_tag="VHV99_28795"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HEX3639408.1"
                     /translation="MLELLSSHACHGGEQRIYRHDSQTVGLPMRFSVYLPPQALQANA
                     NVPALFYLAGLTCTEETFPLKGGAQRFAAQRGIALIAPDTSPRGAGVPGESAAWDFGV
                     GAGFYVDATQQPWAKHYRMYSYVRDELREAVLANLPVDGARLGIFGHSMGGHGALMLA
                     LRNPEIYRSVSAFAPIAAPSQCPWGVKAFSGYLGDDREAWKQYDSSELVAQASRKFAE
                     GILVDQGLADQFLAEQLNPDVFEAACKAAGQPLTLRRHEGYDHGYYFISTFIEDHIAH
                     HAKVLL"
     gene            complement(1390..2172)
                     /locus_tag="VHV99_28800"
     CDS             complement(1390..2172)
                     /locus_tag="VHV99_28800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEX3639409.1"
                     /translation="MFEYDFMVNAFAASGIVAVLAGIVGYFLVMRGQTFAGHALSHVG
                     FTGATGAVLIGISPIWGMIGFTLAAGVGMGALGERLAGRDVAIGVILSLSLGFGLLFL
                     HFFTAYATQVTALLFGNVLGVNASTLGVLAALGVVSLLALAAIMRPLLFASLQPELAE
                     AKGVSLRLVSVLFLSIAALAVAACTQIVGVLLVFTLMVGPAAAAQNMTTRLSTGLVLA
                     AVFALVQAWLGLTLAFYTDWPTSFWITVLSAVVYGGSLLRRR"
     gene            complement(2165..3142)
                     /locus_tag="VHV99_28805"
     CDS             complement(2165..3142)
                     /locus_tag="VHV99_28805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3639410.1"
                     /translation="MNETGRSTPANASTSASSSARANAPVLELEHVTLELGGRTILRD
                     TGFVVNQGEFIGVLGPNGAGKTTLMRAVLGLVPAASGAIRVLRQPVERGNASIGYMPQ
                     TRSALAGRRVRGRDFVAMAADGHRWGLPHADSKTRADVERVLDLVGGRQLAQRPLSEL
                     SGGERQRLLLAQCLLGNPKLLLLDEPLISLDPHHQKSVVELVRRVQQELGIAVLFSAH
                     ELNPLLHALDRVLYLGSGVAALGTVDEVITRPVLSRLYGSPIDVMRVNGRIFVMSGDV
                     EVEKHDHEHEHDENGGHDHGHSHSHDHGHSHGHSHSHSHDSRDGHTHDV"
     gene            complement(3139..4113)
                     /locus_tag="VHV99_28810"
     CDS             complement(3139..4113)
                     /locus_tag="VHV99_28810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter solute-binding protein"
                     /protein_id="HEX3639411.1"
                     /translation="MKKFGSMLNGARRALKLSKAMAMGAAAATAVTLSHGALAADAKI
                     PVVTAENFYGDVVQQLGGERVDVTSILSNPDQDPHLFEASPKTARALQHASLVVYNGA
                     DYDPWMEKLLAASKGTKRTTIVAADLVGRKSGDNPHLWYDPATMPKVARAVSEALVAA
                     DPAHKSAYDANLAKFLDSLQPIDAKVAGLHGRYAGVPVTATEPVFGYMSDAVGLSMRN
                     LRFQLATMNDTEASAADIAAFERDLREKRVRVLIYNSQATEALTKRMLKLAQQSKVPT
                     MSVTETEPAGKTYQTWMLTQLDALGTALAAGDASGANAAASTAKGKTQ"
BASE COUNT          779 a         1364 c         1356 g          739 t
ORIGIN      
        1 cggcaacacc aaggtgatgc gtcaggcgct ggagtgcacg cacaagggct ggggccagtc
       61 gttcatcatc ggcgtggcgg cggcgggcga ggaaatcagc acgcgtccgt tccagctggt
      121 gacgggccgc gagtggaaag ggtcggcgtt cggcggcgcg cgcggccgca ccgacgtgcc
      181 gaagatcgtc gactggtaca tggaaggcaa gatcaacatc gacgatctga tcacgcaccg
      241 tctgccgctc gaacgcatca acgagggctt cgacctgatg aagaagggcg aatcgatccg
      301 ctcggtcgtg ctgtactaac gcggcactaa cgcggcacta acgcggcact aatgcggcac
      361 taatgcggca gtaacgcagc acgaacgccg catcaatgcg gcgccggtgc ggcgtcaatg
      421 cagcactaat acggggccga cccagcacag aaaggagcat gtggatgctt gaactattgt
      481 cgtcgcatgc ctgccatggc ggcgagcagc ggatctaccg gcacgactcg cagaccgttg
      541 gtttgccgat gcgtttctcg gtgtatctgc cgccgcaggc cttgcaggcc aatgcgaatg
      601 tgcctgcgct gttttatctt gccggtctga cctgcaccga agaaaccttt ccgctcaaag
      661 ggggcgcgca gcgcttcgcg gcgcaacgcg gcatcgcgct gatcgcgccg gacaccagtc
      721 cgcgcggtgc gggcgtgccg ggcgaaagcg cggcgtggga cttcggcgtg ggcgcgggct
      781 tttacgtcga cgcgacgcag cagccatggg cgaagcacta tcgaatgtac tcgtacgtgc
      841 gcgacgaact gcgcgaagcg gtactggcga atctgccggt ggacggtgcg cgtctgggca
      901 tcttcgggca ttcgatgggg ggacacggcg cgttaatgct ggcgctgcgc aatccggaga
      961 tttatcggtc ggtgtcggcg ttcgcgccga tcgccgcgcc ttcgcagtgc ccgtggggcg
     1021 tgaaggcgtt cagcgggtat cttggcgacg accgtgaagc gtggaagcag tatgactcga
     1081 gcgagctggt tgctcaggca tcgcgcaaat tcgcggaagg gattctggtc gatcaggggc
     1141 tggcggatca gttcctcgcc gagcagttga atccggatgt gttcgaagcc gcttgcaagg
     1201 ccgcgggaca gccgttgacc ttgcgtcgtc acgaaggcta cgaccacggg tattacttca
     1261 tctcgacttt catcgaggac cacatcgcgc atcacgcgaa ggtgttgctt tgaagtagag
     1321 aaagcccgga acatgagcgg cggcatgcag atgtgccgcc gctaccgctg ggttttggcc
     1381 gaccccgttt caacgccgcc gcaacaaact cccgccatac accacagctg acagcacggt
     1441 gatccagaag ctcgtcggcc aatccgtata gaacgccagc gtcaatccca accacgcctg
     1501 caccagcgca aacacggcgg cgagcacgag tccggtggaa agccgcgtcg tcatgttttg
     1561 cgcagcagca gccggcccga ccatcagcgt gaacaccagc agcacgccga cgatctgcgt
     1621 gcatgccgcc accgccaggg cggcaatcga cagaaatagc accgacacca ggcgcaacga
     1681 cacgcccttg gcttcggcca gttccggttg caacgaagca aacagcagcg gccgcatgat
     1741 cgccgcaagc gccagcaaac tgaccacgcc caacgccgcc agcacgccga gcgtcgacgc
     1801 attcacgccg agcacgttgc cgaacagcag cgcggtgacc tgggtcgcgt acgcggtgaa
     1861 gaagtgcagg aacagcaagc cgaagccgag cgacagcgac aggatcacgc cgatcgccac
     1921 atcgcgtccg gcgagccgct cgcccagcgc gcccatgccg acgccggccg ccagcgtgaa
     1981 gccgatcatc ccccagatcg gcgagatacc gatcagcacc gcaccggtcg ccccggtgaa
     2041 accgacgtgc gagagcgcgt ggcccgcgaa ggtctgtcca cgcatcacca gaaaatatcc
     2101 gactatcccc gccagcaccg cgacgatccc cgacgccgcg aacgcgttca ccatgaaatc
     2161 gtattcaaac atcgtgcgtg tgtccgtcgc gtgagtcgtg ggagtgggaa tgcgaatggc
     2221 cgtgcgagtg gccgtgatcg tgcgaatgcg aatggccgtg atcatggccg ccattctcgt
     2281 cgtgttcgtg ctcgtgatcg tgtttttcga cttcgacgtc gcccgacatc acgaagatcc
     2341 ggccgttcac gcgcatcaca tcgatcggcg agccatagag acgcgacagc accggcctcg
     2401 taatgacttc atcgacggtg ccgagcgcgg cgacgccgct gccgagatac agcacgcgat
     2461 cgagcgcgtg cagaagcgga ttcagttcat gcgccgagaa cagcaccgca atgccgagtt
     2521 cctgctgcac ccgccgcacc agttcgacca cgcttttctg atgatgcgga tcgagactga
     2581 tgagcggttc atcgaggagc agcagcttgg gattgccgag caggcattgc gcaagcagca
     2641 gccgctgacg ttcaccgccc gacagttccg ataacggccg ctgcgccagt tgacggccgc
     2701 ccaccagatc gagcacccgc tcgacatccg cgcgagtctt gctgtctgcg tggggcaggc
     2761 cccagcggtg tccatcggct gccatggcga cgaaatcgcg gccgcgcacg cggcgcccag
     2821 ccagcgcact gcgcgtctgc ggcatatagc cgatcgacgc gttgccacgt tcgaccggct
     2881 gcctcagcac gcggatcgcg ccgctcgcgg ccggcacgag accgagcacg gcgcgcatca
     2941 gcgtggtttt gcccgcgccg ttcggcccga gcacgccgat gaattcgccc tggttcacca
     3001 cgaagccggt gtcgcgcaga atcgtgcggc cgccgagttc gagcgtcacg tgctcgagtt
     3061 cgagcacggg cgcattggcg cgtgcgctgc ttgatgcact cgttgatgca ttagccggcg
     3121 tgctgcggcc agtctcgttc attgggtttt tcctttggcg gtggaagccg ccgcattcgc
     3181 tccgctcgca tcgcccgcgg ccagcgccgt gcccagcgcg tcgagctggg tcagcatcca
     3241 cgtctgatag gtcttgccgg ccggttcggt ttcggtgacg ctcatggtcg gcaccttcga
     3301 ttgctgcgcg agcttcaaca tgcgtttggt cagggcctcg gttgcctggc tgttgtagat
     3361 cagaacgcgc acgcgctttt cgcgcagatc gcgttcgaac gcggcgatat cggccgcgct
     3421 tgcttcagtg tcgttcattg tcgccagctg gaagcgcaga ttgcgcatcg agaggccgac
     3481 cgcgtcggac atatagccga acaccggctc ggttgccgtt accggcacgc cggcgtagcg
     3541 gccatgcaga ccggcgactt tggcgtcgat cggttgcaac gaatcgagaa acttcgcgag
     3601 attcgcatcg tacgccgact tgtgcgccgg gtcagccgcg acgagcgcct cgctcaccgc
     3661 gcgcgccact ttcggcatgg tcgccgggtc gtaccagagg tgcggattat cgccgctctt
     3721 cctgccgaca aggtccgcgg cgacgatcgt ggtgcgcttc gtgcccttcg aggcggccag
     3781 cagtttttcc atccacggat cgtagtcggc gccgttgtag accacaaggc tcgcatgctg
     3841 tagcgcgcgc gccgtcttcg gactggcttc gaacagatgc ggatcctggt ccggattgct
     3901 gaggatgctc gtcacgtcga cgcgctcgcc gcccagttgc tgcacgacgt caccgtagaa
     3961 gttttccgct gtcaccaccg ggattttcgc atccgcggcc aacgcgccat gactgagcgt
     4021 gacggcagta gcggcagccg cacccatggc catggccttc gacagcttca gcgcacggcg
     4081 cgccccgttc aacatcgagc cgaatttctt catcgttgtt tcctgctgcg tgagaattga
     4141 atgaactaca aaccatgggc gcggcgcgct ttcacggtaa tgcacggctt cgtgcatggc
     4201 tcaagccgac gcgccggccg gtcgcgcagc gcgggcgc
//
LOCUS       DATASH010001088         4047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6716526,
            whole genome shotgun sequence.
ACCESSION   DATASH010001088 DATASH010000000
VERSION     DATASH010001088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6716526"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1039
                     /locus_tag="VHV99_28815"
     CDS             <1..1039
                     /locus_tag="VHV99_28815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX3639412.1"
                     /translation="NHWPTEYGGPGWNAVQKFIFENECALAGAPRVVPFGVNMLGPVL
                     IRYGNDAQKRHWLPRILDGSDWWCQGYSEPGAGSDLASVKTTAVRGSDAQGEHYVVNG
                     QKTWTTLGHYANMIFCLVRSATDVRKQEGISFLLIDMNTPGVDVRPIITLDGEHEVNE
                     VFFTDVRVPVENLVGEENKGWTYAKYLLTYERTNIAGVGFSVAAFNRLRKIAAKQQRH
                     GRPLAEDPAFAARMARVEIDLENMKTTNLRVIAAVAGGGVPGAESSMLKIRGTEIRQE
                     ISSLTRRAMGPYAQPFIEEALHEGFEGAPVGPAEAASAASLYFNNRKLSIFGGSNEIQ
                     KNIISKMILGL"
     gene            1052..2182
                     /locus_tag="VHV99_28820"
     CDS             1052..2182
                     /locus_tag="VHV99_28820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX3639413.1"
                     /translation="MNFQHTEDRRMLADTLNRFVAEQYGFETRDRIAGSAEGFSAELW
                     NRFAELGIIGALFDEAEGGFGGGGFDIAVVFESLGRGLVVEPFLDTLMVGQAIARDGS
                     EAQRTTLAELIEGSRLVALAHGEPDGHYELSRVTTRAERNGNTWKLNGAKAVVQQGEN
                     ASLLLVSARTEGAGDAEAGITLFLVPRDAAGVGVRGYRKIDGGRAAEITFDNVTLADD
                     ARLGASGEGFATLEYAVSCGVLALCSEAVGAMDVTKDYTLEYLRTRKQFGVPIGSFQA
                     LQHRMADLLLEIEQARSAVINAAAALGAERTVRERAVSAAKYSIGRIGARVAEECIQL
                     HGGIGMTWELPLAHYAKRLVMIDHQLGDEDHHLERYIALGRG"
     gene            2349..2720
                     /locus_tag="VHV99_28825"
     CDS             2349..2720
                     /locus_tag="VHV99_28825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007584360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639414.1"
                     /translation="MFETGKEYSREDIHRVTGGCKQAFLPVKGGKVVAARLRQDLNPY
                     APGVIVCDSSAAARAAGRTLARQTDPLPVFVRTATDRFRYMGEYITDESLTAPVDYAK
                     YVENTGFTLGQISRVIEMKRR"
     gene            2863..3828
                     /locus_tag="VHV99_28830"
     CDS             2863..3828
                     /locus_tag="VHV99_28830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HEX3639415.1"
                     /translation="MHRIGFVVFPDFYVMGFAAITAFELANLVLGEPAYEVTALSETG
                     GMVPTSSGIRVETQPLGDAIFDTVMFGSGLETDVASPALSDFIKRSLKTSRRIAAPCT
                     GAFILAAAGALDGRRATTHWRFARDLQRRFPKVIVDEDQIFVVDGPIWTSAGMTATID
                     MALAMIEKDHGKDVSRTVARKLVVYHRRAGGQSQFSALLELEPKSDRIQKAIDYANAN
                     LRNPLSVEELADVASLSPRQFSRAFTAETGQSPAKAVEHLRVEAARLMLEQGRHSMDV
                     IADEVGLSDRERMRRAFLRTLGQPPQAIRRFGRQTRLPAEQADRT"
BASE COUNT          800 a         1206 c         1288 g          753 t
ORIGIN      
        1 caatcattgg ccgacggagt acggcggccc cggctggaac gccgtgcaga aattcatctt
       61 cgagaacgaa tgtgccttgg cgggagcacc gcgcgtcgtg ccgttcggcg tcaacatgct
      121 cggcccggtg ttgatcagat acggcaacga cgcgcaaaag cgccactggt tgccgcgcat
      181 tctcgatggc tcggactggt ggtgccaagg ttactcggaa ccgggcgcgg gctcggacct
      241 cgcatcggtc aagactactg ctgtacgtgg cagcgacgcg caaggcgagc attacgtcgt
      301 caacggtcag aagacgtgga ccacgctcgg tcactacgcg aacatgatct tctgcctcgt
      361 gcgcagcgca accgacgtgc gcaagcagga aggcatcagc ttcctgctga tcgacatgaa
      421 cacgccaggc gtcgacgtgc ggccgatcat cacgctcgac ggcgagcacg aagtcaacga
      481 agtgttcttt accgacgtgc gcgtgcccgt tgagaacctc gtgggcgaag agaacaaggg
      541 ctggacctac gccaagtatc tgctcacgta tgagcgcacc aatatcgcgg gcgtcggctt
      601 ctcagtggcc gccttcaacc ggctgcgcaa gatcgccgcg aaacagcagc gccacggccg
      661 gccgctggcg gaagatcccg cgtttgcggc acgcatggcg cgcgtcgaga tcgatctgga
      721 gaacatgaag accaccaatc tgcgcgtgat cgcggcggtg gccggcggcg gcgtgccggg
      781 cgccgagagt tcgatgctga agattcgcgg caccgagatc cgccaggaaa tctcctcgct
      841 cacgcgccgc gcgatgggac cgtatgcaca gccgttcatc gaggaggcct tgcacgaggg
      901 cttcgaaggc gcgccggtcg gccccgccga agccgcgagc gcggcctcgc tctacttcaa
      961 caaccgcaag ctgtcgatct tcggcggctc caacgaaatc cagaagaaca tcatttccaa
     1021 aatgatcctc ggactgtaag gggcgcatgc catgaatttc cagcacacag aagaccgccg
     1081 catgctggcg gatacgctca atcgttttgt cgccgagcaa tacggcttcg agacgcgcga
     1141 ccggatcgcg ggctcggctg aaggttttag cgccgaactg tggaatcgct tcgcggaact
     1201 cggcattatc ggcgcgttgt tcgatgaagc agagggcggg ttcggcggcg gcggtttcga
     1261 tatcgctgtc gtattcgaga gcctgggccg tggcctcgtg gtcgaaccgt ttctcgatac
     1321 cctgatggtc ggacaggcga tcgcgcgtga cggcagcgaa gcgcagagaa ccacgctggc
     1381 cgaactgatc gaaggctcgc gtctggttgc gctcgcgcat ggcgagccgg acggccacta
     1441 cgaactatcg cgcgtgacga cgcgcgccga acgcaacggc aacacatgga agctgaacgg
     1501 cgcgaaagcg gtcgttcagc aaggcgagaa cgcatcgctc cttctcgttt ccgcgcgcac
     1561 ggaaggtgcc ggcgatgcag aagccggcat cacgctcttt ctcgtcccgc gcgacgcagc
     1621 gggggtcggc gtgcgcggct atcgcaagat cgacggcggc cgcgccgccg agatcacctt
     1681 cgataacgtc acacttgccg atgacgcgcg gctcggcgcg tccggcgagg gcttcgcgac
     1741 gctcgaatac gcggtcagtt gtggcgtgct ggccttgtgt tccgaagcag tcggcgcgat
     1801 ggatgtgacg aaagactaca cgcttgaata cttgcgcaca cgcaagcagt tcggcgtgcc
     1861 gatcggcagc ttccaggcat tgcagcatcg catggcggat ctgctgctcg aaatcgagca
     1921 ggcgcgctcg gcggtgatca atgccgcggc ggcgcttggg gccgaacgca cggtgcggga
     1981 acgggcggtt tcggcggcga aatacagtat cggcaggatt ggagcgaggg tcgccgagga
     2041 atgcattcaa ctgcatggcg gtatcggcat gacgtgggaa ttgccgcttg ctcactacgc
     2101 gaagcgcctc gtgatgatcg accatcagct tggcgatgag gatcatcatc tcgaacggta
     2161 tatcgcgctc ggcagagggt gagcgcgggc atcatatccg tgacgcatgt cccgaggtcc
     2221 gctcaacccg catgctttct ttatcaggaa gctcaccctt tatacaaatg atttaggtgc
     2281 aggtcgatat catcgctccg cttgatgcca ctgcgttaca gccaggcgtc attcttcgga
     2341 gaacaatcat gtttgaaact ggcaaagagt attctcggga agatattcat cgagtcacgg
     2401 gaggatgcaa acaggcattc ttgcctgtga agggcggcaa ggtcgtcgcc gcgcgattgc
     2461 gtcaggatct gaatccgtat gccccgggtg tgatcgtctg tgatagcagc gccgccgcac
     2521 gtgcggccgg tcgaacgctt gcgcgacaga ccgacccgct tcctgttttt gtccgaacag
     2581 caacggaccg attccgttac atgggcgagt acatcactga tgaatcgttg actgcgccgg
     2641 tcgactacgc aaagtacgtg gaaaacaccg gattcacgct tggtcagatt tcgcgcgtga
     2701 tcgagatgaa gcgccgttga tctcaccggg gtcttcgatt cgcaagcgtc tcttcgccgg
     2761 ctgggttgtt gctcgtgccc agccggcaaa catcaagtca tcgttgactg cgtcccaaac
     2821 ggcgtatata caggccattt aggacatcgc gtgggacggc atatgcacag aatcggtttc
     2881 gtcgtgttcc ccgatttcta tgtgatgggt tttgcggcaa ttacggcgtt cgaactcgcg
     2941 aacctcgtgc tcggtgaacc ggcgtacgaa gtgacggctc tttcggaaac gggcggaatg
     3001 gtgcccacct cgtcaggaat tcgggtagag acgcagcctt tgggcgacgc cattttcgat
     3061 acggtgatgt tcggttcggg tcttgaaacc gacgttgcgt cccccgcact gagcgatttc
     3121 atcaaacgtt cgctcaagac atcgaggcgt atcgcggcgc catgcaccgg cgctttcata
     3181 ctggcggcag cgggcgctct ggacggccgg cgcgcaacaa cccactggcg attcgcgcgc
     3241 gatctgcaac ggcgctttcc gaaagtgatc gtcgacgaag atcagatctt cgtcgtggac
     3301 ggaccgatct ggacatcggc gggtatgacc gccaccatcg atatggcgct tgccatgatc
     3361 gagaaggatc acggcaagga cgtgtcgcgc acggtcgcgc gcaaactcgt cgtctatcat
     3421 cggcgcgcgg gcggccagtc gcaattctcc gcgttgctcg agctggagcc gaagtccgac
     3481 aggatccaga aggccatcga ctacgcgaac gccaatctgc gcaacccgtt gtccgtggag
     3541 gagctcgccg acgtcgcgag cctgagcccg cgccagttca gtcgagcctt caccgcggag
     3601 accggccagt cgcctgccaa ggccgtcgag cacctgcgcg tggaagccgc gcggctgatg
     3661 ctcgagcagg ggcgccattc gatggatgtg atcgccgatg aagtcggcct gtccgaccgg
     3721 gagcgcatga gacgcgcgtt cctgcgcaca ctcggccagc cgccgcaggc cattcggcgc
     3781 ttcggcaggc aaactcgcct gccggcggag caggccgacc gtacctgatt cagggatgcc
     3841 tgatggagaa gatatcctgg tgcctttcgc tcgatgagac agcgagaaaa ctttgggccg
     3901 gacctccaga tggtcatcaa cgccgggcaa tcaatgcaca aatagggtgc cagttccgca
     3961 tgcacgggtg cgcgccatca ctggaacagg gcagacaccc actgcgacgc cgcgaaatgc
     4021 ggcgatttcc gtttggcacg cttctcg
//
LOCUS       DATASH010001089         2192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_385009,
            whole genome shotgun sequence.
ACCESSION   DATASH010001089 DATASH010000000
VERSION     DATASH010001089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385009"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2192
                     /locus_tag="VHV99_28835"
     CDS             <1..>2192
                     /locus_tag="VHV99_28835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_059641583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX3639416.1"
                     /translation="LVQNRVNQALPRLPEDVQRLGITTIKSSPTLTMVVHLISPNNRY
                     DMTYLRNYALLNVKDRLARIQGVGEVQLWGSGDYAMRVWLDPQKVAQRGLTATEVVNA
                     IREQNIQVAAGVIGASPSVPGTQLQLSVNARGRLKTEGEFGDIIVKTAPDGGVTYLRD
                     IARIELAASEYGLRSLLDNKPAVALAINQAPGANSLAISDQVRSAMKELAEDMPTGVE
                     YKIVYDPTQFVRSSIEAVIHTLLEAIALVVIVVIVFLQTWRASIIPLIAVPVSIVGTF
                     SLLLAFGFSINALSLFGMVLAIGIVVDDAIVVVENVERNIENGLSARDATYKAMQEVS
                     GPIIAIALTLVAVFVPLAFMTGLTGQFYKQFAMTIAISTVISAFNSLTLSPALAALLL
                     RSHGAKEDVLTRVMNRVLGRFFKGFNKVFHRGSTEYGRGVKGVLRRKGAMLAVYAILL
                     GATVLISRVVPGGFVPAQDKEYLIAFAQLPNGASLDRTEKVIRDMSAIALKQPGVESA
                     VAFPGLSVNGFTNSSSAGIVFVTLKPFKDRGNKKLSAGAIAGALNQQYGAIKDSFVAV
                     FPPPPVLGLGTLGGFKMQLEDHGAVGYAELNKAAEAFVKKAAQTPELGPTFSSYQINV
                     PQLNVDLDRVKAKQLGVPVTDVFNTMQIYLGSLYVNDFNRFGRVYQVRVQADAPFRQR
                     ADDILQLKTRNAAGDMVPLSSLVTVTPTYGPEMVVRYNGYTAADINGGP"
BASE COUNT          399 a          611 c          734 g          448 t
ORIGIN      
        1 ctggtgcaga accgcgtcaa ccaggcgctg ccgcgcctgc cggaagacgt gcagcgcctc
       61 ggcatcacga ccatcaagag ttcgccgacg ctgacgatgg tggtccacct gatctcgccg
      121 aacaatcgct acgacatgac gtatctgcgc aactacgcgc tgttgaacgt caaggaccgg
      181 ctggcgcgga ttcagggcgt gggtgaggtg caactgtggg gttcgggcga ctacgcgatg
      241 cgcgtctggc tcgatccgca gaaggtcgcg cagcgcggtt tgaccgcgac cgaagtggtc
      301 aacgcgatcc gtgagcagaa cattcaggtg gcagccggtg tgatcggcgc atcgccttcg
      361 gtaccgggca cgcaattgca gttgtcggtg aatgcgcgcg gccgtttgaa gactgagggt
      421 gaattcggcg acatcatcgt caagaccgcg ccggatggcg gcgtgacgta cctgcgtgat
      481 atcgcgcgta tcgaactggc ggcgtcggaa tacggcttgc gttcgttgct cgacaacaag
      541 ccggcggttg cgctcgccat caatcaggcg ccgggtgcca actcgctggc gatttccgat
      601 caggttcgtt ccgcgatgaa ggaactggcc gaagacatgc cgacgggcgt cgaatacaag
      661 atcgtctatg acccgacgca gttcgtgcgc tcgagtatcg aggcggtgat ccacacgctg
      721 ctcgaagcga tcgcgctagt ggtgatcgtg gtgatcgtgt tcctgcaaac gtggcgtgcg
      781 tcgatcattc cgctgattgc ggtgccggtg tcgattgtcg gtacattctc gttgctgctt
      841 gcgttcggct tctcgatcaa cgccttgtca ttgttcggca tggtgctcgc catcgggatc
      901 gtggtggacg atgcgatcgt ggtggtggag aacgtcgagc ggaacatcga aaacggcctc
      961 agtgcgcgcg atgcaacgta taaggcgatg caggaagtca gtgggccgat tatcgccatc
     1021 gcgctgacgc tggtcgccgt gttcgtgccg ctcgctttca tgaccggctt gacggggcag
     1081 ttctacaagc agttcgcgat gactatcgcg atctcgacgg tgatctcggc attcaactcg
     1141 ctgaccttgt cgccggcttt ggccgcgctg ctgttgcgca gccacggcgc gaaggaagac
     1201 gtcctgacgc gtgtgatgaa tcgcgtgctg ggccgctttt tcaagggctt caacaaggtg
     1261 ttccatcgcg gctcgacgga gtatggccgt ggcgtgaagg gcgtgctgcg ccgtaaaggg
     1321 gcgatgctcg cggtgtacgc gatcctgctg ggcgcgactg tgctgatctc gcgcgtcgtg
     1381 ccgggtggct tcgtgccggc gcaggacaag gagtacctga ttgcgtttgc gcagttgccg
     1441 aacggcgcgt cgctggatcg cacggaaaaa gtgatccgcg acatgagcgc gattgcgttg
     1501 aagcagccgg gcgtggaaag cgcggtggcg ttcccgggtt tgtcggtgaa cggtttcacc
     1561 aatagttcga gcgcgggcat cgtgttcgtc acgttgaagc ctttcaagga ccgcggcaac
     1621 aagaagctct ccgccggtgc gattgccggt gcgttgaacc agcaatacgg tgcgatcaag
     1681 gattcgttcg tcgcggtgtt cccaccacct cctgtgctcg gactcggcac gctgggcggc
     1741 ttcaagatgc aattggaaga tcacggcgcg gtgggctatg cggagttgaa caaggcagcg
     1801 gaagcgttcg tgaagaaagc ggcgcaaacg cctgaacttg gaccgacgtt ctcgagctat
     1861 cagatcaacg tgccgcaact gaacgtcgac ctggatcgcg tgaaggccaa gcagctcggc
     1921 gtgcccgtca cggacgtgtt caacacgatg cagatctatc tgggctcgct gtacgtgaac
     1981 gacttcaacc gtttcggccg cgtctatcag gtgcgggtgc aggcggacgc gccgttccgt
     2041 caacgtgccg acgacatcct gcaactgaag acgcgtaacg cggcgggcga catggtgccg
     2101 ttgtcgtcgc tggtcacggt gacgcctacc tatggtccgg aaatggtggt gcgctataac
     2161 ggctataccg ccgccgacat caacggtgga cc
//
LOCUS       DATASH010001090         2823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4541902,
            whole genome shotgun sequence.
ACCESSION   DATASH010001090 DATASH010000000
VERSION     DATASH010001090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4541902"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="VHV99_28840"
     CDS             complement(<1..856)
                     /locus_tag="VHV99_28840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEX3639417.1"
                     /translation="MQSTLRQPRVALGWIVFLLIAVVGLFYVKWFPYYNRAFVAASQH
                     SIGKSILMGTSAGAPAASWQAAIDYALAYGKAIWQAMVLGLLLGSAVQALIPPQWVAR
                     ALGRSDFSSVVKGGLMSLPGMMCTCCAAPVVAGLRARQAAPGAAIAFWLGNTALNPAT
                     LIFLGFVLGWQWMGLRLALGLVMVFGLGYLVNRMVTPKEAEASREAMAQLVTSDVPGT
                     AFARWVKILGTMTLRLVPEYIVLVLVLGAARTWLFPHIGPDIDNSLLWIIALSIAGTL
                     FVIPTAGEV"
     gene            complement(888..1955)
                     /gene="mnmH"
                     /locus_tag="VHV99_28845"
     CDS             complement(888..1955)
                     /gene="mnmH"
                     /locus_tag="VHV99_28845"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HEX3639418.1"
                     /translation="MKNLLVSLDQAGDFDEIIDVRTPLEFAEDHIPGALNAPVLSNEE
                     RVLVGTTYKQVSPFEGTRIGAALVARNIAHHLETTFADRPRNWRPLIYCWRGGKRSGS
                     VTTLFNMIGWPARQLNGGYKSYRRATLDTLDSLPKRFNYIALVGPTGSGKTRLLEALN
                     RAGAQTLDLEALAAHRGSLLGAWAGIAQPSQKRFDTLLVSALRTFDPLRPVFVEAESR
                     RIGSITLPPALLETFQQGACVEVLSSHEDRAAFLLHDYAHLFDDPESLKAQLQKMIGL
                     HSRERVAGWQQLVDENSRAELARELIERHYDPAYARSSHQHFVQLPRALQMKFRPNDA
                     DVVEQAKALLAQLGNSAFALI"
     gene            complement(2229..>2823)
                     /gene="selD"
                     /locus_tag="VHV99_28850"
     CDS             complement(2229..>2823)
                     /gene="selD"
                     /locus_tag="VHV99_28850"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEX3639419.1"
                     /translation="VAIGVVDPRRVKRNADARAGDVLILGKPLGVGVLSAALKKDQLD
                     SNGYAAMIAATTKLNRPGAALSMLDGVHALTDITGFGLLGHTLELARGSNLTARVRYA
                     DLPWLPDVVGFAEAGIFTGASGRNWDAYGKDIVLPTSLPSTARTLLTDPQTSGGLLVS
                     CAPDVVDEVLALFRADGFDEARVIGEMVSGERQVEVV"
BASE COUNT          542 a          920 c          869 g          492 t
ORIGIN      
        1 gcacttcgcc cgcggtggga atcacgaaca acgtcccggc gatcgacagc gcgatgatcc
       61 acagcaggct gttatcgatg tccggtccga tgtgcggaaa cagccacgtg cgcgccgcgc
      121 cgagaaccag caccagcacg atgtattcgg gaacgagccg cagcgtcatc gtgccgagga
      181 ttttcaccca gcgtgcgaag gcagtgccgg gcacatcgct ggtgaccagt tgcgccatgg
      241 cttcgcgcga cgcttcggct tccttcggcg taaccatccg gttcaccaga tagccgaggc
      301 cgaacaccat tacgaggccc agggcgagcc gcaagcccat ccattgccag ccgagcacga
      361 agcccaggaa gatcagcgtg gccggattga gggccgtgtt acccaaccag aacgcgatcg
      421 ccgcaccggg cgccgcttga cgggcgcgca agccggccac gaccggcgcg gcgcagcacg
      481 tgcacatcat gcccggcaac gacatcaggc cgcccttcac cacgctgctg aaatcgctcc
      541 ggccgagtgc gcgtgcgacc cactgcggtg gaatcagcgc ctgcaccgcc gaacccagca
      601 gaagccccag caccatcgct tgccagattg ccttgccgta ggccagcgcg taatcgatgg
      661 ccgcctgcca cgacgccgcc ggcgcgcctg ccgacgtgcc catcaggatc gatttgccga
      721 tggagtgctg gctcgcggcg acgaaggcgc ggttgtagta cgggaaccac ttcacataga
      781 agaggccgac gacggcgatc agcagaaaca cgatccagcc aagggcgaca cgaggttgcc
      841 ggagagttga ttgcatgatg gatggtcttg agctttcgtt gtttgaatca gatgagggcg
      901 aacgcgctgt taccgagttg agcaagcagt gcttttgcct gctcgacgac gtcggcgtcg
      961 ttgggccgaa atttcatttg caacgcacgc ggcagttgta cgaaatgctg atggctgctg
     1021 cgcgcatacg ctggatcata gtgccgttcg atcagctcgc gagcgagctc ggcccggcta
     1081 ttttcatcga caagttgttg ccagcccgcg acacgttcgc ggctatgcaa accgatcatc
     1141 ttctgcagtt gcgctttcaa cgattcggga tcgtcgaaca gatgcgcata gtcgtgcagc
     1201 aggaacgccg cgcggtcttc gtggctcgac aatacttcca cgcaggcacc ctgctggaac
     1261 gtttcgagga gcgcgggcgg cagcgtgatc gaaccgatcc gccggctctc cgcttcgacg
     1321 aacacgggcc gcagcggatc gaacgtgcgc agggcgctga ccagcaacgt gtcgaagcgc
     1381 ttttgcgacg gttgcgcaat gcccgcccac gcgccgagca gcgaaccgcg atgggcagcc
     1441 agtgcttcga ggtccagcgt ttgcgcgccc gcccgattca gcgcttctag caggcgcgtc
     1501 ttgccgctgc ctgtagggcc caccagcgcg atgtagttga acctcttcgg caacgagtcg
     1561 agggtgtcca gcgttgcccg ccgatagctt ttatagccgc cgttcaactg gcgcgcgggc
     1621 cagccgatca tattgaagag tgtcgtgacc gaacctgagc gtttcccgcc gcgccagcag
     1681 tagatcagcg gccgccagtt gcgcggccgg tccgcgaaag tcgtttccag atgatgcgcg
     1741 atgttgcgcg caaccagcgc cgcgccgatc cgcgtgcctt cgaacggcga aacctgcttg
     1801 tacgtcgtgc cgaccagaac acgctcttcg ttgcttagca ccggcgcatt gagcgcgccg
     1861 ggaatatggt cttcggcgaa ctcgagaggc gtgcgcacat cgatgatttc atcgaaatca
     1921 ccggcttggt cgaggctgac gaggaggttt ttcaaagggt atggacggaa cggaccggcg
     1981 ggagaagcga aattatcgca tgcgccgtca atgccggctg gtggctggtt cggccctgtt
     2041 tacaggcgct gttccaggag gcctgcttcc ggggcttgct tcacggaccc gcttcaaggt
     2101 cccgcttcaa ggacccgctt ccggacctgc tccaagggca tgcttcacgg acctgctcca
     2161 agacctgctc caagacctgc gcgtcgcccc gcgtcgcccc gcgtcgccca taaaaaccgg
     2221 cttgttcctc agaccacctc gacttgccgt tctccgctga ccatttcgcc gatcacgcgc
     2281 gcttcgtcga aaccgtcggc acggaacagg gcgagtacct catcgaccac gtccggtgcg
     2341 cacgaaacca gcagaccgcc cgaagtttgc ggatcggtga gaagcgtacg ggccgtcgac
     2401 ggcaaggagg tcggcaacac gatgtcttta ccgtatgcgt cccaattgcg ccccgacgcg
     2461 ccggtaaaaa tcccggcctc cgcgaagccg accacatcgg gcaaccaagg caaatccgca
     2521 taacgcacgc gagcggtcag attcgaaccg cgcgccaatt ccaatgtgtg gcccagcaaa
     2581 ccaaagccgg tgatgtccgt gagggcatgc acgccgtcga gcatggagag cgccgcgccc
     2641 ggccggttga gcttggtggt ggccgcgatc atcgcggcgt agccgtttga atccagctgg
     2701 tcttttttca acgccgccga cagcacgccc acgccaagcg gcttgccgag gatcagcacg
     2761 tcgcccgccc gcgcatctgc attgcgtttg acgcgccgcg gatcgaccac gccgatcgca
     2821 acg
//
LOCUS       DATASH010001091         4094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5531524,
            whole genome shotgun sequence.
ACCESSION   DATASH010001091 DATASH010000000
VERSION     DATASH010001091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5531524"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1150
                     /locus_tag="VHV99_28855"
     CDS             <1..1150
                     /locus_tag="VHV99_28855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEX3639420.1"
                     /translation="IERKPAADVPQPATPAPAHKPAASRPREHVLTATQPATQAPTVV
                     AASDAAASASVAASSPSVNANAGANATGAASAPAASAPQASAGVKFSVPPSAELAYDT
                     FYNGVRNQPGTIHWTSNAQSYEMVISVPLPFVGTFVYSSHGRIDAFGLAPDQYIEKRG
                     HRPEDVAIFNRTDKKIAFTRTPASLPLPDGAQDRFSMVMQLASLVRGDPAAYKPGVTR
                     QFFVVDSNSGENWPIETIGDETIRTAQGYLETRHFKRLPRHDGDLRRIDVWLAPSLGW
                     LPARIMQTEPNGTEFELVWRGKLNADDAGSPATSPDGTIGSGASAGSAGSAGSGSSNS
                     LGSTDNAGNSSPDNSTIQLPANTPANTPEAAPVSPVDSTVPGNQPEKP"
     gene            1310..2098
                     /locus_tag="VHV99_28860"
     CDS             1310..2098
                     /locus_tag="VHV99_28860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX3639421.1"
                     /translation="MQVKINGIETRYVLSNEGGGPWLTFIHQLGGDLSVWDQLAGYFR
                     DDYTVLRYDVRGHGETAVASEPFTIADLSSDLVELLDALDAPTTHIVGMSMGGMIAQQ
                     CALDHASRIDTLTLANTAGATLPEARAVWDQRAAAARTNGVTSLVPATLSRWLTPDFQ
                     AAHPEAVEQIRDVLSHTLSEGYAMACEALRDFDVRSKLGTIRRPTLAVAGRHDTGTPP
                     AATQAIADAIEGAQFELLEAAHLAPIEQSHRFAALLETFLERPV"
     gene            2216..2473
                     /locus_tag="VHV99_28865"
     CDS             2216..2473
                     /locus_tag="VHV99_28865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3567 domain-containing protein"
                     /protein_id="HEX3639422.1"
                     /translation="MQMIYNSPNYCVVEFPPQEGPLAMKSGGYEIVDKNMQREIYIDG
                     AMAARFREHVQKLIEEEPSLDEVDEFLGQFDSLMHQPVILH"
     gene            2803..3873
                     /locus_tag="VHV99_28870"
     CDS             2803..3873
                     /locus_tag="VHV99_28870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEX3639423.1"
                     /translation="MNQPAQTATPVRPDVVYTRGAALPALLKSRILILDGAMGTMIQR
                     YKLDEARYRGERFKDYGRDIKGNNELLSITQPQIISEIHEQYLAAGADILETNTFGAT
                     TVAQADYGMEELAVEMNLESAKLARAACDKYSTPDKPRFVAGAIGPTPKTASISPDVN
                     DPGARNVTFDELRAAYYEQAKALLDGGCDLFLVETIFDTLNAKAALFALDELFEDTGE
                     RLPIMISGTVTDASGRILSGQTVEAFWNSLRHARPLTFGLNCALGAALMRPYIAELAK
                     LCDTYVSCYPNAGLPNPMSDTGFDELPADTSGLLKEFAQAGLVNIAGGCCGTTPEHIA
                     AIAKALAEIKPRQWPTQYRDAA"
BASE COUNT          842 a         1359 c         1181 g          712 t
ORIGIN      
        1 catcgagcgc aagcccgccg ccgacgtgcc gcaacccgcg acgccagccc cggcgcataa
       61 gccagccgcg agcaggccgc gtgaacatgt gctcacggca acgcaaccgg caacgcaggc
      121 gcccacggtc gtggccgcat cggatgccgc cgcgagcgca tcggtcgcag cgagctcccc
      181 cagtgtcaac gccaatgcgg gcgcaaacgc caccggtgca gccagcgccc ccgccgccag
      241 cgcgccccag gcttcagcgg gcgtgaaatt ctccgtgccg ccatccgcag aactggcata
      301 cgacacgttc tacaacggcg tgcgcaatca gcccggcacg atccactgga cgagcaacgc
      361 ccagagctac gagatggtca tctccgtgcc gctgccgttc gtcggtacgt tcgtgtattc
      421 gagccatggc cgcatcgacg cattcggcct cgcgccggat cagtacatcg aaaagcgcgg
      481 ccatcgtccg gaagacgtgg cgattttcaa tcgcaccgac aaaaagatcg ccttcacgcg
      541 gacaccggcc tcgctgccgc tgcccgacgg cgcgcaagac cgattcagca tggtgatgca
      601 actcgccagc ctcgtgcgcg gcgaccccgc cgcctacaag ccgggcgtga cgcggcaatt
      661 cttcgtggtc gatagcaaca gtggcgagaa ctggcccatc gagacgatcg gcgacgagac
      721 gatccgcacc gcgcagggtt acctcgagac gcgccacttc aaacgcttgc ctcgccatga
      781 cggcgatcta cgccgcatcg acgtatggct cgcgccgtcg ctcggttggc tgcccgcgcg
      841 catcatgcag accgagccga acggcacaga atttgaattg gtgtggcgcg gcaagctcaa
      901 tgcggacgac gccggtagtc cggccaccag ccccgatggc accatcggct caggtgcctc
      961 tgctggttct gctggttctg ctggttctgg tagctctaat agccttggca gcaccgataa
     1021 cgccggcaat tcttcaccag acaattcgac aatccagctc cccgccaata ctcccgcaaa
     1081 tacgcccgaa gccgcccccg tctccccggt cgattcgacc gttcccggca atcagcccga
     1141 gaagccctga ccccgagtag ggcctcgaac acgtcgctga aacttccgcg acaggagtca
     1201 ctcgcacaag agctgcaacc gttcgcgcgg ccgccgcgta tatctcgtga tgacggcaga
     1261 agcgtgcgca aacggcccta tccactgcgt tccccttcaa ggagcccgca tgcaagtgaa
     1321 aatcaacggc atcgagacgc gctatgtact gagcaatgag ggcggcggcc cgtggctgac
     1381 gttcattcat cagctcggcg gcgatctgtc tgtgtgggac cagctcgccg gctatttccg
     1441 tgacgactac accgtgttgc gctacgacgt gcgaggccat ggcgaaacag cggtagcgag
     1501 cgagcctttc accattgccg atctgtccag tgatctcgtc gagttgctcg atgcactcga
     1561 cgcacccacc acgcatattg tcggcatgtc gatgggcggc atgatcgctc agcaatgcgc
     1621 gctggaccat gcgtcgcgca tcgatacgct gacgcttgcc aacaccgccg gcgcaacctt
     1681 gcctgaggcc cgcgcggtat gggatcaacg cgcagcggcc gcgcgcacga acggcgtgac
     1741 atcactggtg cccgcgacat tgagccgctg gctgacacct gattttcagg cagcgcatcc
     1801 cgaggcagtc gagcagatcc gtgatgtgct aagtcatacg ttgtcagaag gctacgccat
     1861 ggcgtgcgaa gcgctgcgcg atttcgatgt acgcagtaag ctgggaacaa tccgtcgccc
     1921 gacgttggcc gtggcgggcc gtcacgacac gggcacgcca cctgctgcta cgcaggcaat
     1981 agcagacgcc atcgaaggcg cgcagttcga actgctcgag gccgctcatc ttgcgcccat
     2041 cgagcaatct caccgttttg ctgcactgct tgaaacgttt cttgaaaggc cggtctaacc
     2101 ggtaggttcg gaactttaca agcgtcaatc cggacccacc ccttgaattc cacaccacaa
     2161 gctccaccta tggcgcagga atggccagga gccaacggaa ataaggagtg tcgccatgca
     2221 aatgatctac aacagcccca actattgtgt cgtcgagttc ccgccacagg aaggtccgct
     2281 ggccatgaag tcgggtggct acgagatcgt cgacaaaaat atgcagcgcg aaatctacat
     2341 tgacggcgca atggcggcgc gttttcgcga gcatgtgcaa aaattgatcg aagaggaacc
     2401 gtcactggat gaggtagacg aattcctcgg gcagttcgac agcttgatgc atcaaccggt
     2461 gattcttcac taactgaagg gtttggcgta acggtttggc gacatacatc gaccgcgccg
     2521 accggctcag ccggcggcat ttacaagtcg gtcagcggta atgacacggc ccagcaggtt
     2581 ttcacctgac tgggccgttt tctttgctga ccgtcttctt tcctgaccgt cttctttgct
     2641 ggccgtcttc tttgctggcc gtcttctttg ctgaccgtct aatggcataa cggccacagc
     2701 cacgccgctc tccggcccgc gcccggcgaa actaccccgc agcggctaca atgacaggtt
     2761 tcccgaaaac ccgaaaaagc ccggcgcaag cccacgtccg ccatgaacca gcctgctcaa
     2821 accgccacgc cagtccgccc cgacgtcgtc tacacgcgtg gcgcggcgct gcccgcgctg
     2881 ctcaagtcac gcatcctgat cctggacggc gcaatgggca cgatgatcca gcgctacaaa
     2941 ctcgacgagg cccgctatcg tggcgagcgc ttcaaggact atgggcgcga catcaaaggc
     3001 aacaacgagt tgctgtcgat cacccaaccg cagatcatca gcgagattca cgagcagtac
     3061 ctcgcagcgg gcgcggacat cctcgagacc aacacgttcg gcgccaccac ggttgcgcag
     3121 gccgactacg ggatggaaga gctcgccgtc gagatgaatc tcgaatccgc gaagctggca
     3181 cgcgccgcct gcgacaagta ctcgacgccg gacaagccac gctttgtcgc cggcgcgatc
     3241 ggaccgacgc cgaaaacggc gagcatttcc cctgacgtaa acgatccggg cgcgcgcaac
     3301 gtgacgttcg acgaactgcg cgccgcgtac tacgagcagg cgaaggcgtt gctcgacggc
     3361 ggctgcgatc tgttcctcgt cgaaacgatt ttcgacacgc tcaatgccaa ggccgcgcta
     3421 ttcgcgctgg acgaactgtt cgaagacacc ggcgagcgcc tgccgatcat gatctcgggc
     3481 accgtcaccg atgcatcggg ccggattctc tcaggacaaa ccgtcgaggc cttctggaac
     3541 tcactgcgtc atgcgaggcc gctcacgttc ggtctgaact gcgcgttggg cgcagcgctg
     3601 atgcggccat acatcgcgga gctggcaaag ctgtgcgaca cgtatgtgtc gtgctatccg
     3661 aacgccggtt tgccgaatcc gatgagcgat acgggcttcg acgaactgcc ggcggataca
     3721 tcgggattgc tgaaggagtt cgcccaggca ggtctcgtga atatcgccgg cggatgttgc
     3781 ggcacgacgc cggagcatat tgcggcgatt gccaaggcgc tggccgaaat aaagccgcgc
     3841 caatggccga cccaataccg cgacgcagcc tgagtccgac ggctcgttct cgagctgccc
     3901 ttctttcgcg atcgaacccg acgcacgtaa ccgcacgcaa ccgaaccgta cccacgccat
     3961 gaccgatcac accatgcgcc tttccggcct cgagccgttc aacgtcacgt cagggacact
     4021 cttcatcaac gtcggtgagc gcaccaacgt gaccggttcg aaggcgttcg cgcgaatgat
     4081 cctgaacgac cagt
//
LOCUS       DATASH010001092         2767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6123775,
            whole genome shotgun sequence.
ACCESSION   DATASH010001092 DATASH010000000
VERSION     DATASH010001092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6123775"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..1666
                     /locus_tag="VHV99_28875"
     CDS             125..1666
                     /locus_tag="VHV99_28875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HEX3639424.1"
                     /translation="MNSSNPPGAPVSQTRSFSTVFLIEMWERFGYYGMAALLVLFMID
                     KLNFTDSHATLTWGAFTALVYAAPSIGGWIGDKILGARRTMIFGAVVLAAGYFMLALP
                     NEQLSYMYASLGVIVVGNGLFKANAANLVRRIYEGDDARIDSAFTIYYMAVNIGSTVS
                     MLATPWIKDHWGWHTAFAVCCAGMLLAVLNYFIMFRTLAHIGSAPDAGPIRWKRVGAV
                     ALGGIVLGAATMFVLQHKAIAVACVYTAGVAILAIFGYMLLKCERSERAGLVAALILT
                     AQVILFFVFYVQMSTSLTLFALRNVDPRFMLFGETLFTWSAAQFQALNPIWIMLLSPV
                     LALLYTKLAKSGKDVPVAVKYAFGFAVVAAGFFVYAASGNYAVNGRVSSWFMVGGYGL
                     YSLGELLVSGLGLAMIARYVPARMSGFMMGAYFVATGVSQYLGSVVANFAQMPVGDLD
                     PLESLPLYTRLFTGLGWLAAVGALVAVLLLPLMRKLSREHQRCSDEARESARQTAALS
                     GTAAD"
     gene            1890..>2767
                     /locus_tag="VHV99_28880"
     CDS             1890..>2767
                     /locus_tag="VHV99_28880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018423505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX3639425.1"
                     /translation="MDIHIAVEGRHDLSGQIYRQLRAGILAGRLAGGTRLPSTRDLAT
                     QLGVSRKTTLDVFERLLSEGYLSARAGSGTFVADGLERLPVERSPHARGAESARERAK
                     AKQQAAARAQPLWEQMPETLPLPRPTVASPYDFVGGATDKSLFPFDVWRRCVNHALRM
                     QARGPGTYRDVAGEQELRLAISRYLAFNRAVTSNWDDVIVTQGAQHALDLMARITLRP
                     GEIAALEDPGYPPAHACFTATGARVVPVPVDSEGLIVSKLPDKARLVYVTPSHQFPLG
                     MPMSLERRVELLEWAQ"
BASE COUNT          437 a          866 c          923 g          541 t
ORIGIN      
        1 cagaaccgga atgcacggcc gccgtttgac cgaggttgat ccgcggaccg ttttcaagaa
       61 tccgtttttc gcgccgcgtg gaccacccac gcgccgccgc aaccataaaa gcaagaggtc
      121 catgatgaat tcatccaatc cgcccggcgc accggtctcg cagacccgtt cgttctcgac
      181 ggtcttcctg atcgagatgt gggagcgctt tggttattac gggatggccg cgctgctggt
      241 cctgttcatg atcgacaagc tgaacttcac cgacagccac gccaccctca cgtggggcgc
      301 gttcacggcg ctggtgtacg cggcgccctc gatcggcggc tggatcggcg acaagattct
      361 cggcgcgcgc cgcacgatga tcttcggcgc ggtcgtgctg gcggccgggt atttcatgct
      421 ggcgctgccc aatgagcaat tgagctacat gtacgcatcg ctcggcgtga tcgtggtggg
      481 caacgggctt ttcaaggcga atgccgcgaa tctcgtgcgc cgcatctatg aaggcgacga
      541 cgcgcgcatc gacagcgcat tcacgatcta ctacatggcg gtcaatatcg gctcgacggt
      601 atcgatgctc gcgacgccgt ggatcaagga ccattggggc tggcacacgg cattcgcggt
      661 ctgctgcgcg ggcatgctgc tggcggtgct gaactacttc atcatgttcc gcacgctcgc
      721 gcatatcggt tccgcgccgg acgccggacc gatccgctgg aaacgcgtcg gcgcggtcgc
      781 gctgggcggt atcgtgctcg gcgcggcaac catgttcgtg ctgcagcaca aggcgattgc
      841 cgtggcctgc gtctacacgg cgggcgtcgc gattctggcg atcttcggct acatgctgct
      901 gaagtgcgaa cgctcggagc gtgcgggtct ggtcgccgcg ctgatcctca ctgcgcaggt
      961 gatcctgttc ttcgtgttct acgtgcagat gtcgacgtcg ctcacgctgt tcgcgctgcg
     1021 caacgtcgat ccgcgcttca tgctgttcgg cgagacgctg ttcacgtgga gcgccgcgca
     1081 gttccaggcg ctcaatccga tctggatcat gttgctgagc ccggtgctcg cgctgctcta
     1141 tacgaagctc gcaaagagcg gcaaggacgt gccggtggcg gtcaagtacg cattcggctt
     1201 cgcggtggtg gcggccggct tcttcgtgta tgcggcgagc ggcaactacg cggtgaacgg
     1261 acgcgtgtcg tcgtggttca tggtgggcgg ctacggcctg tattcgctcg gcgaattgct
     1321 ggtgagcggg ctgggtctcg cgatgatcgc gcgctacgtt ccggcgcgca tgagcggctt
     1381 catgatgggc gcttacttcg tggcaaccgg cgtgtcgcag tatctgggca gcgtggtcgc
     1441 gaacttcgcg cagatgccgg tgggcgatct ggacccgctc gaatcgttgc cgttgtacac
     1501 cagactgttc acaggtctgg gctggctcgc ggcggtcggc gcgctggttg ccgtgctgct
     1561 gttgccgttg atgcgcaagc tttcgcgtga acatcagcgt tgcagcgacg aggcgcgcga
     1621 gagcgcgcgg caaaccgctg cgctgagcgg tacggcggca gattaagcca gtcggcaact
     1681 gaagaaatgg cgcgcttcgt gtcgcacggc gcgccatttt gcgttctggg tagtgtgctc
     1741 gcggcgtgcg ctacgtacct cgcatctccg gacctcgcgg cggcttgact gcgccgaccg
     1801 cactccaata gcggcgaccg aggtaagctt gcagttggca ccggtcttcc ggatcaaccg
     1861 tgtcccggat attttaggta accactcgga tggacatcca cattgcggtc gaagggcgtc
     1921 acgatctgtc cggacagatc taccggcagt tgcgtgccgg catactcgca gggcgcctcg
     1981 ccggtggcac acgcttgcct tccacgcgcg atctcgcgac gcaactgggc gtgtcgcgca
     2041 agaccacgct cgacgtattc gagcggctgc tctcagaagg ctatctgagc gcccgtgccg
     2101 gttcgggcac cttcgtcgcc gatggtctcg agcgtttacc ggtggaacgc tcgccgcatg
     2161 cacggggggc cgagtcggcg cgcgagcggg caaaggcgaa acagcaagcc gcggcgcgcg
     2221 cccagccgct gtgggagcag atgcctgaga ccttgccgct gccgcggccc acggtcgcct
     2281 cgccgtacga tttcgtcggc ggcgcgactg acaaatcgct attccccttc gatgtctggc
     2341 gccggtgtgt gaaccacgca ttgcgtatgc aagcgcgcgg cccaggcacg tatcgcgacg
     2401 tggccggcga gcaggaattg cgcctcgcga tttcgcgtta cctggcgttc aaccgcgcgg
     2461 tcacgagcaa ctgggacgac gtgatcgtca cgcaaggcgc gcagcacgcg ctcgatctga
     2521 tggcacgcat cacgttgcgt cccggcgaga tcgcggcgct cgaagacccc ggctatccgc
     2581 cggcccacgc atgcttcacg gccacgggcg cacgcgtggt gcccgtaccg gtggacagcg
     2641 aaggcctgat cgtgagcaag ctcccggaca aggcgcggct cgtctacgtg acgccctcgc
     2701 atcagttccc gctcggcatg cccatgagtc tggagcggcg cgtcgagttg ctcgaatggg
     2761 cgcagaa
//
LOCUS       DATASH010001093         2552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3948325,
            whole genome shotgun sequence.
ACCESSION   DATASH010001093 DATASH010000000
VERSION     DATASH010001093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3948325"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /gene="purU"
                     /locus_tag="VHV99_28885"
     CDS             complement(<1..865)
                     /gene="purU"
                     /locus_tag="VHV99_28885"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HEX3639426.1"
                     /translation="MSTDHSFILKLSCADRPGIVHAVSGFLFERGSNILDSAQFGDSR
                     TGEFFMRVHFQQVGGDPGLEALRTSFTTLAEQFGMHWELHDASVKPRVVIMVSKIGHC
                     LNDLLFRYRTGQLGIEIPAIISNHKEFYQLAASYDIPFHHFPLMGGTPDAKTAQEARV
                     LEVIDEHQADLVVLARYMQILSPQLCESLAGRAINIHHSFLPSFKGAKPYYQAFDRGV
                     KLIGATAHYVTTDLDEGPIIEQEVERVDHSMTPEQLTAIGRDVECVTLARAVKWHVEH
                     RVVLNGSKTVVF"
     gene            complement(1052..1930)
                     /locus_tag="VHV99_28890"
     CDS             complement(1052..1930)
                     /locus_tag="VHV99_28890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4743 domain-containing protein"
                     /protein_id="HEX3639427.1"
                     /translation="MTLPCITAARRFDVLAHLPFWIDAEQVGWIRSTDVPLLTRWPDV
                     FEIDGTRVTLAPSFNTVDLRSAALGSVIGALAAEGRIPGWRNETYAIRNAFDAPPLAF
                     IERAASRFFGTLTYAVHLNGVVEYADSVPREAGPRMAAPQLWIARRSDTKATDPGMLD
                     NVVAGGIGWGFGIEATIVKECWEEAGIPEEIAARAVAGRIAHVLQSLPEGTQAEQIFI
                     YDLALPADFAPRNQDGEVGEHRLARIDDVARWIEEGVMTVDASLATLDCLLRRRWIDE
                     DACAGIETLFVPPVFA"
     gene            complement(2019..>2552)
                     /locus_tag="VHV99_28895"
     CDS             complement(2019..>2552)
                     /locus_tag="VHV99_28895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004530934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEX3639428.1"
                     /translation="RGISQGRAAGLVAALGFAAGITFHTTLAALGVAALLRSSPVAFE
                     VIKLAGAAYLVWIGIKALRSQGLATAHERAPQPLMAVFRQSVLGNLLNPKVTLFFVVF
                     LPQFVQPHGTQSVTVQMLELGVLFMLQTVVVFSLFGVCAGMIGGWLKRRPRVGVWLDR
                     LAGATFIAIGIRVALRD"
BASE COUNT          508 a          845 c          760 g          439 t
ORIGIN      
        1 gaaacaccac cgtcttgcta ccgttcaaca cgacgcgatg ctcgacgtgc cacttcaccg
       61 cgcgcgcgag cgtcacgcat tcgacgtcac ggccgatcgc cgtcagttgc tccggcgtca
      121 tgctgtgatc gacgcgctcc acttcctgct cgatgatcgg accttcgtcg agatccgtcg
      181 tcacgtagtg cgcggtcgcg ccgatcagct tgacgccgcg atcgaacgcc tggtaatacg
      241 gcttcgcgcc cttgaagctc ggcaggaacg aatggtgaat attgatcgcg cggccggcga
      301 gcgattcgca caactgcggc gacagaatct gcatgtagcg cgcgagcacc accagatccg
      361 cttgatgttc gtcgatgact tcgagcacgc gcgcttcctg cgcggtcttc gcgtcgggcg
      421 tgccgcccat tagcgggaag tgatggaacg gaatgtcgta gctggccgcg agctgataga
      481 actccttgtg gttcgagatg atcgccggaa tctcgatacc cagttggccg gtgcggtagc
      541 gaaacagcag gtcgttcagg caatggccga tcttcgacac catgatgacc acgcgcggtt
      601 tcaccgacgc atcatgcaat tcccagtgca tgccgaactg ctcggcgagc gtcgtgaacg
      661 acgtacgcaa cgcctccagg cccggatcgc cacccacctg ctggaaatgc acgcgcatga
      721 agaactcgcc ggtgcggcta tcgccgaact gcgccgagtc gagaatattg ctgccacgct
      781 cgaacagaaa gcccgaaacc gcgtggacga tgccgggccg gtcggcgcac gacagtttga
      841 ggataaagct gtgatcggtc gacatgacct gggtgactcc ctttcttcaa tgcgtggttt
      901 gttcggtgtg tcttgttcga tacagcgtgc tgcacgcgga actcaagctt ttcgtcgcgc
      961 agcgcgctac tagcggctca ttgcgcttat gcgactacta gcgcctcact acgcttatgt
     1021 gcctactagc gcgtcattag cgcttatgcg cctacgcaaa caccggcggc acgaacagcg
     1081 tctcgatgcc cgcacacgcg tcctcatcga tccagcgacg gcgcagcagg caatccagcg
     1141 tggcaagact cgcatccacg gtcatcacgc cttcctcaat ccagcgcgcc acgtcgtcga
     1201 tgcgcgcgag ccggtgttcg cccacttcgc catcctgatt gcgcggcgcg aaatcggccg
     1261 gcagcgcgag atcgtaaata aaaatctgct cggcctgcgt gccttccggc aacgattgca
     1321 gcacatgcgc aatgcggcca gccacggcgc gcgcggcgat ctcctcggga atgccggctt
     1381 cttcccagca ctccttgacg atcgttgcct cgatgccgaa gccccaaccg atcccgcctg
     1441 ccacgacgtt gtcgagcata cccgggtcgg tcgccttggt gtcgctgcgg cgcgcgatcc
     1501 ataattgcgg ggcggccatc cgcgggccag cctcccgcgg gacgctatcc gcgtattcta
     1561 cgacgccgtt cagatgcacc gcgtaggtca gcgtgccgaa gaagcgcgac gccgcgcgtt
     1621 caatgaaggc gagcggcggc gcatcgaagg cattgcggat cgcataggtc tcgttgcgcc
     1681 agccgggaat gcggccttcg gcggcaagcg caccgatcac ggaaccgagc gccgcgctgc
     1741 gcagatcgac cgtgttaaag gaaggcgcga gcgtcacgcg cgtgccgtcg atttcgaaca
     1801 catcgggcca gcgcgtcagc aacggcacat cggtcgagcg aatccagccg acctgttcgg
     1861 cgtcgatcca gaacggcaga tgcgcgagga cgtcgaaacg acgcgcggcg gtaatgcaag
     1921 gcaaagtcat ttaaaactcc aaactgttct tcggtcacgc tgaatcgttg tcggccgtgc
     1981 ggttgctgtc aaccgtgcaa ccgtatcggc cgcctcgatc aatcacgcaa ggcaacccga
     2041 atcccaatcg cgatgaacgt cgcgccggcc agccggtcga gccacacgcc cacgcgcggc
     2101 cgccgcttca gccagccgcc gatcatcccc gcgcacacgc cgaagagcga gaacaccacc
     2161 acggtttgca acatgaacaa cacgccgagt tcgagcatct gcacggtgac gctttgtgtg
     2221 ccatgcggct gcacgaattg cggcaggaac acgacgaaga acagcgtcac cttcggattc
     2281 agcagattgc cgagcacact ctgacgaaac accgccatca acggttgcgg cgcgcgctca
     2341 tgcgccgtgg cgagcccctg gctgcgcagc gccttgatgc cgatccagac cagatacgcg
     2401 gcaccggcca gcttgatcac ttcgaacgca accggcgacg agcgcagcaa cgcggccacc
     2461 ccaagcgcag ccaacgtggt gtgaaaggtg atgccggcgg caaatccgag cgcggcgacg
     2521 agacccgccg cacggccttg cgaaataccg cg
//
LOCUS       DATASH010001094         6051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1971028,
            whole genome shotgun sequence.
ACCESSION   DATASH010001094 DATASH010000000
VERSION     DATASH010001094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6051
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1971028"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VHV99_28900"
     CDS             <1..282
                     /locus_tag="VHV99_28900"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HEX3639429.1"
                     /translation="GVQPSMGTAGDAYDNAMCESFFGTLEAELLMREHFDTHDQARRR
                     IFSFLELVQRTPPAQQHRLSCTARIRKPACAGANAIAVRVAHRRAASRS"
     gene            complement(386..955)
                     /locus_tag="VHV99_28905"
                     /pseudo
     CDS             complement(386..955)
                     /locus_tag="VHV99_28905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011003443.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
     gene            1357..2232
                     /locus_tag="VHV99_28910"
     CDS             1357..2232
                     /locus_tag="VHV99_28910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX3639430.1"
                     /translation="MTSPTQVISPLRQRMMDDMRMRRLAPKTQANYLRVVREFTIFLG
                     RSPDTATVEDLRRYQLHLVDHGVSPVSLNAAITGLKFFFATTLGQQELMAKMRPVYLP
                     RTLPVILSREEVGRLIAAATNLKHQTVLALAYGTGLRASEVAALKVGDIDSERMTLRV
                     EQGKGQKDRYAMLSPILLERLRVWWRVARAQGKMLDGGWLFPGLNPIEPLSTRQLNRA
                     VHAAAEAAGIDKRVSMHTLRHSFATHLLEQKVDIRVIQVMLGHKKLETTALYAQVATD
                     ILREVVSPLESLRTG"
     gene            2262..3428
                     /locus_tag="VHV99_28915"
     CDS             2262..3428
                     /locus_tag="VHV99_28915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HEX3639431.1"
                     /translation="MADVFRSLGPAWHQSANLSLGQLKVMSAIEQCRTAALGGHVLRC
                     QGCAGIEIAYNSCRNRHCPRCQAGAARRWLEAREADLLPVEYYHLVFTLPAAISAIAY
                     YNKAVVYGLLFDIAAETLRTIAADPRHLGAQIGATLVLHTWGSALTHHPHVHGIVPGG
                     GLSSDRARWVACKPGFFLPVRVLSRLFRRRFLEELGASYRRGQLRFFGEYAVLNNAAA
                     FAGWVAPMRATEWVVYAKRPFAGPTAVLAYLSRYTHRVAISSQRLIALDERGVTFRWK
                     DYRVDGRTRNKTMTLEADEFVRRFLLHVLPGGFHRIRHYGLLANPSRRENLAKIRELL
                     GIVSAPIEVGANETTTVAVPPTFVCRHCGSPMTIIDILQRSRPIRAPPAERAYA"
     gene            complement(4032..4283)
                     /locus_tag="VHV99_28920"
     CDS             complement(4032..4283)
                     /locus_tag="VHV99_28920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018432656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-binding protein"
                     /protein_id="HEX3639432.1"
                     /translation="MSHVVVERSFPTPQSDTDLSVVEERLAPCSELYGVVWKRSVVSV
                     DRRHMICEYEAPDAETVRKVQREAGAAFDHIWSGEVIES"
     gene            complement(4340..>6051)
                     /locus_tag="VHV99_28925"
     CDS             complement(4340..>6051)
                     /locus_tag="VHV99_28925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3639433.1"
                     /translation="LARDSKVSRLELSALSADQVAALIEASRSDIGPEQAMKESGGNP
                     LYVLELTRAKRANIEASTRTVDALIADRLAPLDAPTRDLLSFAASIGRGFAPEQLAQL
                     LDSPLPGILSSLSELQRRGIVTAATQTEFDFAHDLVRQTVYRMLPQPQRRAIHHQIAR
                     QLLAASKHDTRLHGEVVHHATLAGDSRMTASACVEASNQCLAVFATTEARAVAERGLA
                     HARSLPQGSERVRVEIRLLTARLVAVASSGDACPASMEHEFEQAIQEAEALSLHAEVV
                     EGLHSLSWLTQQANDIERTRQVTIRAETAARKADATTRCKQLAVTGRCLLELERELPR
                     ARCVLHEAGVLAERLNLRVVELMWGAALLARAEGELDIGCAQLSDAVRQARAIGDHWR
                     EYQCLVWLATMNFERGAYFQVTQSAGSIVVAAQRMGDSGAPYAEVIREIASMRLTSGD
                     PRTFVFEGLDALREADDKRHLCYALNEVALTCLDCRCSDQASAHAREAIAVADVLDAP
                     TERIVARAVSVEAAFTAGEKERAERLLSSLRELLSDKPITPRSHKALQRLFERYPVTT
                     TIV"
BASE COUNT         1158 a         1910 c         1775 g         1208 t
ORIGIN      
        1 ggcgtgcagc catcgatggg caccgcaggc gacgcctatg acaatgcgat gtgcgagtcg
       61 ttcttcggca cgctcgaagc cgagctgctc atgcgcgaac acttcgatac ccatgatcag
      121 gccaggcggc gcatcttctc cttcctggaa ttggtacaac gtacgccgcc tgcacagcag
      181 catcggttat cgtgcaccgc tcgaattcga aagcctgcat gcgcaggagc aaacgccatc
      241 gcagtgcggg ttgcccaccg ccgggcagcg tcacggtcgt gacaggcgac ccgccgcccg
      301 gccgtggaca acccgagatc cagcaagcca tggaggatca acaacacatt aaacccaacc
      361 gcaaatctgt ccgtccaacc gggtcaacct catataacaa acccaggtcg cttatccctt
      421 cccgaaaggc cgtttcatca ccatatcccg catcggccag cacgataccc ggcacaatcc
      481 cggatgcaat cgcttcacgc agttgggcca gtgcgatctg tggtttggtt gcgaaggcct
      541 gatcgtcagg aatgccggcg cgacgggcac gttcccgatc gtcaatccac tccctgggga
      601 catacagttg ccaggcgata ggcaggctgc cgcattgcgt tgcgatcgat aaactcactg
      661 cgacctgaca gttgtcctgt ttgccgagct ggccacagta ctgacgtgca acgcccaccg
      721 aatggcggcc cttctttgga aagcctgtgt cgtcaataat ccagtaatag ccggcctctt
      781 cagcggcatg cgcgcctgga gcgggcatca cccactcgcg cacccgctgc atgatcgctt
      841 tgtcagacca ctccgccttg gcaacaaaat ggtggagcga ttgatgcttc gcactcgcgt
      901 gaagcggatc aatatacgcg gccatcggct cgacgctctt tcttgacagc ggcatgcgtc
      961 gatgctcgcg ctaggggacc ggcgggtcgc ctcgatgggt gactcctcat acggcacgca
     1021 cacgatgcgt cggacgaagg cgtcaatgat ctatcgtcgc tagaagaacc tgcgcgcagt
     1081 tcaattactc cttgggcata ccaagcttca gagcaccgtg cggtaccttg gtgtcgaagt
     1141 cgacaatgcg cttgaaatgg cagaacagac ggaagtttaa gcgttcgcgc ccaactgggc
     1201 ggtgatcgac cgttggtcgc tgtccggcca atggactaaa ccgctcgcgc ggtatgcgct
     1261 ccgttcaaag ctattggtcg gtgaagggta aaacccctcg gtttcctatg ttgggtgatg
     1321 aggcgatccg cctcgttacc caggaggaac cgggccatga catcccccac ccaagttatc
     1381 agtccgttgc gtcagcgcat gatggacgac atgcgcatgc gtcgactcgc gccaaagacc
     1441 caggccaact acctgcgcgt ggtgcgggag ttcaccattt tcctcgggcg ctcacctgac
     1501 accgcaaccg tcgaggatct gcggcgctat caactgcatc tggtcgatca cggtgtatcc
     1561 cccgtttccc tgaacgccgc gattaccggg ttgaagtttt tcttcgccac tacgctcggt
     1621 cagcaggagt tgatggcgaa gatgagaccg gtttatcttc cgcgtacgtt gcccgtcata
     1681 ctcagccgcg aagaggttgg ccggctgatc gcggcggcga ccaacctgaa gcatcaaacg
     1741 gtgctggcgc tcgcctacgg aacaggatta cgcgccagcg aggtagctgc cctgaaggtc
     1801 ggtgacatcg atagcgaacg catgacgctg cgcgttgagc agggcaaagg ccagaaggac
     1861 agatatgcca tgctgtcgcc gatcctgctc gagcgtttgc gggtctggtg gcgagtcgca
     1921 cgagcccagg gcaagatgct cgacggcggc tggctgtttc ccggacttaa cccgatcgaa
     1981 ccgctcagca cccgccaact caaccgggcg gtccatgctg cggccgaagc tgcgggaatc
     2041 gacaagcgcg tgtccatgca taccttgcgg cacagctttg ccacgcatct gctggaacag
     2101 aaggtcgata tccgggtgat ccaggtcatg ctcgggcata aaaagcttga gaccacagcg
     2161 ctttatgccc aggttgccac cgatattctg cgtgaggtgg ttagtcctct tgagagtttg
     2221 cgtaccgggt agctctcatc gtggcgcatt gcgcgcttga ggtggcagac gtctttcgct
     2281 ctcttggtcc agcgtggcat caatccgcta acctgagcct cgggcagttg aaggttatgt
     2341 cggcgatcga acagtgccgc accgcggcgc tgggagggca cgtgctgcgt tgccaggggt
     2401 gcgccgggat cgagattgcc tacaactcgt gccgcaacag acattgccca cggtgccagg
     2461 ctggcgctgc acgtcgctgg ctggaagcgc gtgaggccga tcttctgccc gtcgagtact
     2521 accacctggt ctttacactg ccggctgcga tcagcgcgat tgcgtactac aacaaggccg
     2581 tcgtctacgg attgctgttc gatatcgcgg ccgaaacgtt gcgcaccatt gccgctgatc
     2641 ccaggcatct cggagcacag attggtgcca cgcttgtcct gcacacctgg ggctccgcgc
     2701 tcacgcatca tcctcatgtg catggcatcg ttcccggtgg cgggttgtcg tccgatcgcg
     2761 cccgatgggt cgcatgcaag ccaggcttct tcctgcctgt acgagtcctt tcccggctgt
     2821 ttcgccggcg cttcctcgaa gagctgggcg ccagctaccg tcggggccag ttgcggttct
     2881 tcggcgagta cgcggtgctc aacaatgccg ccgcctttgc cggatgggtt gcgccgatgc
     2941 gggcaactga atgggtggtg tacgccaagc gcccgtttgc gggaccgaca gcggtgctgg
     3001 cttatctgtc acgctatacc caccgggtcg cgatctccag tcaacgcctg attgccctcg
     3061 acgaacgcgg agtcaccttc cgctggaaag actaccgtgt ggacggacgc acacgcaaca
     3121 agaccatgac cctcgaagca gatgagttcg tacgccggtt cctgttgcac gtgctacctg
     3181 gcggcttcca tcgcattcgc cattacggcc tgctggcgaa cccatcgcgg cgcgagaatc
     3241 tggcgaagat acgtgagttg ctgggcatag tgtctgcccc catcgaagtc ggtgccaacg
     3301 aaacaaccac agtcgcagtg ccgccaacct tcgtttgccg gcactgcggt tccccgatga
     3361 ccatcatcga catacttcaa cgcagcagac ctatccgggc accacctgct gagcgggcgt
     3421 acgcatgaac gcgatcatct caggatgtac acaccgactg tcggctcttc ggcaacgaaa
     3481 gcccatagga gacgtctgcg tccacgactt caaacaggct gtctggtgcc ggccgggtcg
     3541 acgtggttgc aatgcccagc agcgctgctg ccacgatcgc gactatccag gcttgaattt
     3601 gcctgcgttt atatccatca ggctctcaac gggtttgaac aaatcgccat agcggtgaaa
     3661 cgctcagacc atgtcggagc actccgcggt ttcctccctt ggggcttgta ggacgcctgc
     3721 ccgctggcgt ctgggcaagt cgatgtgtcg acggctgagg gcaggcgtcc tacaacccta
     3781 aacacaaacg gcctttcgat cgtgctggcg ttcggccatt gaaacggctg attgacaccc
     3841 aactgcggtc gttccgatct gttgcggacg ggcggcgttg gccgcctgcg ccgccaggat
     3901 cctcgagtcg cgcgcgtccg tcgacggacc gtagccgtga tagcgaacgg cgcgcgcgca
     3961 gcgctcgcaa gtcagcgttt cgcactgttc ctatgcttga cgcggcagct cgtgcaaaag
     4021 cgcgtgtcac ttcagctttc gatgacttcg cccgaccaga tgtgatcgaa ggcagcgccg
     4081 gcttcccgtt gcaccttgcg gacggtttcc gcgtcgggcg cttcatattc gcagatcatg
     4141 tgcctgcgat cgacggaaac cacactgcgt ttccagacga cgccgtatag ttcgctgcac
     4201 ggcgctaggc gttcttccac cacggacaaa tccgtgtccg actgtggcgt cggaaagctc
     4261 ctttcaacga cgacatgcga catgtcagtt ctccctttgc ggcggagcct gccctcgcca
     4321 caaattaatg cgcctgcgtt taaacgatcg ttgtagtgac gggatagcgc tcgaagagcc
     4381 gttgtagtgc cttgtgactt ctgggagtaa tcggtttgtc ggacagcaac tcgcgcaatg
     4441 aagaaagaag gcgctcggcg cgctcctttt cgcccgccgt gaatgctgct tcgaccgaaa
     4501 ccgctcttgc aacgatcctc tcggtcgggg catcaaggac gtcggctacc gcgatcgctt
     4561 cgcgcgcatg agcgcttgcc tggtcgctac atcgacagtc cagacaggtc agcgccacct
     4621 cgttgagggc atagcaaaga tgccgcttgt cgtcagcttc gcgcaatgcg tctagcccct
     4681 cgaagacgaa cgtgcggggg tcgccgctcg tcagacgcat cgacgcgatt tcgcggatga
     4741 cctctgcata aggtgcgccc gaatcgccca ttcgctgcgc ggccacgaca atcgagccgg
     4801 ccgattgcgt gacttgaaaa taagcgccgc gctcgaaatt catggtcgcc agccatacga
     4861 gacactggta ctcacgccag tggtcgccta tcgcccgagc ctgccgcacg gcgtcgctca
     4921 actgtgcgca tccgatgtcc agctccccct cggctcgcgc aagaagcgct gcaccccaca
     4981 tcaactccac gacacgcagg ttcaggcgtt cagccagcac gcccgcttcg tgcaaaacgc
     5041 agcgcgcccg aggcagttcg cgctcgagtt cgagcaggca ccggccggta accgcgagct
     5101 gcttgcaacg cgtcgtcgca tcggctttgc gtgcggccgt ttctgcgcgg atcgtcactt
     5161 gtcgcgtacg ttcgatgtcg ttcgcctgct gcgtcagcca cgacaagcta tgaagaccct
     5221 caaccacctc ggcatgaagc gacagagctt ccgcctcctg aatggcttgc tcgaattcat
     5281 gttccatcga cgcgggacac gcgtcgcccg aactcgccac ggccacgagc cgcgcggtga
     5341 gtaaccggat ttcgacccga acccgctcgc ttccctgcgg cagtgaacgt gcgtgagcca
     5401 gaccgcgttc cgcgacagcg cgagcctcgg tggtcgcaaa aaccgccaaa cactggttgc
     5461 tggcttcgac gcatgcgctt gccgtcatgc gcgagtcccc tgccaacgtc gcgtgatgaa
     5521 cgacttcgcc gtgcagtcgc gtatcgtgct tgctggcggc caggagttgt cgcgcaatct
     5581 gatgatggat cgcgcgcctc tgcggctggg gaagcattcg atagacggtc tgccgcacaa
     5641 gatcgtgtgc gaaatcgaac tccgtctgcg tggccgcagt aacgattccc cgacgttgta
     5701 gttcggaaag gctcgacagg atgccaggaa gcggactgtc gagcagttgc gcaagctgtt
     5761 cgggagcaaa cccacgaccg atcgaagccg cgaatgaaag cagatcgcgc gtgggcgcat
     5821 cgagcggcgc gagccgatcc gcgatcaaag catcgacagt gcgcgtcgag gcttcgatat
     5881 tcgctcgctt tgcgcgcgtc agttcgagca catagagcgg attgccgccg ctttccttca
     5941 tcgcctgctc cgggccgata tcgcttctcg atgcctctat aagcgcggcc acttgatcag
     6001 ccgaaagtgc agacagctcg aggcgcgaca ccttactgtc gcgcgccaac g
//
LOCUS       DATASH010001095         3349 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2563980,
            whole genome shotgun sequence.
ACCESSION   DATASH010001095 DATASH010000000
VERSION     DATASH010001095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2563980"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VHV99_28930"
     CDS             complement(<1..401)
                     /locus_tag="VHV99_28930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HEX3639434.1"
                     /translation="MYVAVKGGERAIESSWRLLDKARRGDTRLAELSVAQIREQLRLA
                     VARVMTEGSVYDEELAALAIKQAAGDLVEAIFLLRAYRTTLPRFGYTQAIDTEAMHVE
                     RRISATFKDVPGGQLLGATYDYTQRLLDFALL"
     gene            complement(401..1024)
                     /gene="phnH"
                     /locus_tag="VHV99_28935"
     CDS             complement(401..1024)
                     /gene="phnH"
                     /locus_tag="VHV99_28935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HEX3639435.1"
                     /translation="MDNSQIALASSLSTLAPGFADPVHDTQVVFRTLLDALSRPGSVG
                     VVENVLPEVQATRAELAAFAALLTLCDYATPVWLAQADTALSSALRFHTGAPLVNEPA
                     QAAFAYIHDASAMPALESFALGAAESPEQSVTLLIRVEALTGGAPVVLSGPGIQHTAT
                     IAPLGLPEHFWRERAALAPLFPCGIDCYLVCGNTLIGLPRTTQAKVN"
     gene            complement(1024..1509)
                     /gene="phnG"
                     /locus_tag="VHV99_28940"
     CDS             complement(1024..1509)
                     /gene="phnG"
                     /locus_tag="VHV99_28940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004535918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HEX3639436.1"
                     /translation="MSATSASPSSALRRAWMAVLARTPRADLEAALERALQGVPPPPY
                     DWLRPPEIGLAMVRGRVGGSGDPFNLGEATVTRATLRLRATGDANAAVGVACHLGRDR
                     RRAELAALADALLQMPEHHADLHRQLIEPFAAQQAAQRVERRQDAAATRVEFFTMVRG
                     D"
     gene            1691..2449
                     /gene="phnF"
                     /locus_tag="VHV99_28945"
     CDS             1691..2449
                     /gene="phnF"
                     /locus_tag="VHV99_28945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HEX3639437.1"
                     /translation="MTSNDNATPGTSLERGAGVAVWRQIEQILATEIAASGFGEEGRL
                     PSEGELAKRFDVNRHTVRRAMLGLAALGLVSVEQGRGTFVQPGAIDYTIGRRTRFTEN
                     LQQQNHAAAGTMLSAARVKAEPNVAKALGLRAGAPVYRIESLHESDGVPLTFARSWYP
                     AARFADLPEVLERTGSTTKAMAELGVTDYLRKWSRIGSVLPEPEVARRLNINRQQPVL
                     WVENVDVDLQGTPIKYGFTHFAADRVQLLVEHDV"
     gene            2446..3168
                     /locus_tag="VHV99_28950"
     CDS             2446..3168
                     /locus_tag="VHV99_28950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1045 domain-containing protein"
                     /protein_id="HEX3639438.1"
                     /translation="MSGAAWSTGARFAVYYAPSRESAWWRVGSAWLGRDAQSGERCVP
                     PQPQALGRPLADLTEAPRRYGWHGTLVAPFRPAEGVTQQHVLEATRDWASTQSPFALP
                     VEAATLGDFVALRPADPDGEANIRHVASSALRTLHPLRATPSAADLARRLAAPLSERQ
                     RALLIEWGYPYVFDEFRFHMTLSSSLADADERATLVSWWQAQTSALGPLTIDHVALFV
                     EPAPGEPFVLWQRVPFQTQEVK"
     gene            3171..>3349
                     /locus_tag="VHV99_28955"
     CDS             3171..>3349
                     /locus_tag="VHV99_28955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism
                     protein/1,5-bisphosphokinase (PRPP-forming) PhnN"
                     /protein_id="HEX3639439.1"
                     /translation="MTGRLIYVMGPSGAGKDSLLSFARSRLMGEPIVFAHRYITRPSG
                     NGEEHIALSVEEFATR"
BASE COUNT          606 a         1022 c         1123 g          598 t
ORIGIN      
        1 agcaaggcga aatccagcag acgctgcgtg taatcgtaag tcgcgccgag caactggccg
       61 ccgggcacgt ccttgaatgt cgccgaaatg cggcgctcca cgtgcatggc ttccgtgtcg
      121 atcgcctgcg tgtaaccgaa acgcggcagc gtggtgcgat acgcacgcaa cagaaagatc
      181 gcctcgacca gatcgcccgc ggcctgtttg atcgcgagcg cggcgagttc ttcgtcgtag
      241 accgaacctt cggtcatgac tcgcgccacc gcgagacgca attgctcgcg aatctgcgcg
      301 acgctgagtt cggccagccg cgtgtcgccg cggcgcgcct tgtcgagcag acgccacgat
      361 gattcgatcg cgcgttctcc acccttgacg gcaacgtaca tcagttcacc ttcgcttgag
      421 ttgtgcgcgg caggccgatc aacgtattgc cgcagacgag ataacaatcg atgccgcacg
      481 gaaagagcgg cgcgagagcg gcgcgttcac gccagaaatg ctcgggcagg ccgagcggcg
      541 cgattgtcgc cgtgtgctga atgccggggc cgctcagcac gaccggcgcg ccacccgtca
      601 gcgcttcgac gcggatcagc agggtcaccg attgctcagg cgattccgcc gcgccgagcg
      661 cgaagctttc caacgccggc atcgcgcttg cgtcgtggat gtaggcaaag gcagcttgcg
      721 cgggttcgtt gacgagcggc gcgccggtat gaaagcgcag cgccgaactc aacgccgtat
      781 cggcttgcgc gagccacacg ggcgtggcgt agtcgcacag cgtcagcagc gcggcgaagg
      841 cggcgagctc ggcccgcgtg gcctgcactt ccggtaaaac gttttcgacg acgcccaccg
      901 agcctggacg tgacagcgcg tcgagcaggg tacggaacac cacctgggtg tcgtgcaccg
      961 ggtctgcgaa acccggtgct agcgtggaca atgatgaagc caatgcaatc tgcgagttgt
     1021 ccatcagtcg cccctgacca tcgtgaagaa ttcaacgcgt gtcgccgcag cgtcctgcct
     1081 gcgttcgacg cgttgtgcgg cttgctgcgc ggcaaacggc tcgatcaatt gccgatgcaa
     1141 gtcagcgtga tgctcgggca tttgcagcag cgcatctgcc agagcagcca gttcggcacg
     1201 gcgacggtcg cggcccagat gacacgcaac gccgacggcg gcgtttgcgt cacccgtggc
     1261 gcgcaaacgc aacgtggcgc gcgtgaccgt ggcttcgccg aggttgaacg ggtcgccgct
     1321 gccgccgaca cggccgcgca ccatcgcgag accgatttcg ggtggccgca gccagtcgta
     1381 ggggggcggc gggacgcctt gcagcgcgcg ttccagcgcg gcttcgagat cggcgcgcgg
     1441 ggtgcgggcc agcacggcca tccaggcgcg ccgcaaggcg gaggaaggcg aggcggaagt
     1501 agcactcatc gaatttcctg ttgatccagc gagtggacat ttgaccattt attcatctaa
     1561 acgtctagac gtttgaaggt atgattgttg ggcgttgtcg cgcccggttg ccgtttcata
     1621 tttccatcac ggcttgcgca ctacaatgca cggcacagtg aatatttgaa cccaggggaa
     1681 tgacagcacc atgacatcga acgacaatgc gacaccgggc acatcgctcg aacgcggcgc
     1741 gggcgttgcc gtctggcggc agatcgaaca aattctggcg acggaaatcg ccgcaagcgg
     1801 cttcggcgaa gaaggccgtt tgccgagcga gggcgaactg gccaaacgct tcgacgtgaa
     1861 ccggcatacc gtgcgccggg ccatgctcgg tcttgccgca ttgggcctcg tcagcgtcga
     1921 gcagggacgt ggcaccttcg tgcagcccgg cgcgatcgac tatacgattg gccgccgcac
     1981 gcggtttaca gaaaacttgc agcagcagaa tcacgcggcg gcgggcacga tgttgtcggc
     2041 cgcgcgcgtg aaggccgagc cgaatgttgc gaaggcgctc ggcctgcggg caggcgcgcc
     2101 ggtctaccgg atcgagtcgc tgcacgagtc ggatggcgtg ccgctcacgt tcgcgcgtag
     2161 ctggtatccg gccgcgcgct ttgcggactt gccggaagtg ctggaacgca ccggcagtac
     2221 caccaaggcg atggctgaac tcggcgtgac cgattatctg cgcaagtgga gccggatcgg
     2281 cagcgtgttg cctgaaccgg aagtggcgcg gcgtttgaat atcaaccggc agcaaccggt
     2341 gttgtgggtc gaaaacgtcg acgtcgatct gcaaggcacg ccgatcaaat acggttttac
     2401 gcattttgcg gccgaccgtg tgcaactgct ggtggagcac gacgtatgag cggcgcggca
     2461 tggagcaccg gggcacggtt tgcggtgtat tacgcaccgt cgcgcgagtc ggcatggtgg
     2521 cgggtgggtt cggcgtggtt ggggcgcgac gcgcaaagcg gtgaacgatg cgtgccgccg
     2581 caaccgcagg ccttggggcg tcctctggcc gatctgaccg aagcgccgcg ccgctatggt
     2641 tggcacggca cactggtcgc gccgtttcgt cctgctgaag gcgtcacgca gcaacatgtt
     2701 ctcgaggcga cgcgtgactg ggctagcacg caaagtccat tcgcgttgcc ggtcgaagct
     2761 gcgacgctgg gcgatttcgt ggcactacgt cctgctgatc cggatggcga agcgaacatt
     2821 cgtcacgttg catccagtgc gctgcgaacg cttcacccat tgcgcgcgac gccctccgcc
     2881 gccgacctcg cgcgccgtct cgccgcgccg ttgagcgaac ggcaacgcgc actgctgatc
     2941 gaatggggat atccgtacgt cttcgacgag ttccgttttc atatgacgct gtccagttcg
     3001 ctcgcggatg cggatgagcg cgcgacgctc gtctcgtggt ggcaggcgca aacatccgcg
     3061 ctcgggccgc tcactatcga tcacgttgcg ctctttgtcg aacccgcacc cggcgagccg
     3121 ttcgttttgt ggcagcgtgt gccgtttcag acccaggagg tgaaataaca atgacaggcc
     3181 gtttgattta cgtaatgggg ccgtccggcg cgggcaagga ttccttgctg agttttgcgc
     3241 gaagccgcct gatgggcgag ccgattgtgt ttgcgcatcg ctacatcacg cggccgagcg
     3301 gcaacggtga agagcatatt gcgctgagcg tcgaagaatt cgcgacgcg
//
LOCUS       DATASH010001096         2071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2564424,
            whole genome shotgun sequence.
ACCESSION   DATASH010001096 DATASH010000000
VERSION     DATASH010001096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2564424"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1365)
                     /locus_tag="VHV99_28960"
     CDS             complement(<1..1365)
                     /locus_tag="VHV99_28960"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HEX3639440.1"
                     /translation="MFRNTARCLDALFVIAGGLLAHGMRFSAPMALSDTERLLIAFNC
                     VLVLLMFPGFGVYETWRGKALPSMLARVAAAWLVVVATALVLAFTLHRMDAVSRLWFG
                     YSTLISGALIIVTKCLVHVALRSVRRRGMNFRTVAIVGAPGFSRTLLAHLDHAPQAGF
                     KPVCVFDTSIEAAGTYGARLKRLPVLTDLNAFAAKVREEHVNEVWLALPLSEEHTIYR
                     FQRIFRHDFVNLRFIPDVRSLSLFNHALVDVVGLPTLNLSATPLSSPQMWPKLIFDRL
                     FAAAALLMLAPVFVALAIGIKLSSPGPVFFRQTRKGVDGQPFAIYKFRSMTVHHEAHG
                     QLTQAARNDARVTKLGRFMRRTSLDELPQFLNVLLGQMSVVGPRPHAVEHDDLYKDQV
                     YGYMNRYRIKPGITGWAQVNGYRGATTKVEKMETRVKFDLFYIHNWSFWFDMKIVFIT
                     IFKGF"
     gene            complement(1412..>2071)
                     /locus_tag="VHV99_28965"
     CDS             complement(1412..>2071)
                     /locus_tag="VHV99_28965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX3639441.1"
                     /translation="APHLELVHLRTEQLLCDSGQRIHHVYFPTTAIISMLSTMEDGSS
                     VEIAAVGREGMTGVPVLTGGETMPNRVQVQCAGFAYRMSAQALKQQFARSDFLRRLML
                     LYMHALLTQVAQTAACNRHHSLNKQLCRWLLIEVDRVASNDLTVTQQLIADMLGVRRE
                     GITEAAGKLHDEGLIHHSRGHIKILDRKGLEARACECYGLVKREFDRLLPRLRQAETV
                     E"
BASE COUNT          418 a          611 c          678 g          364 t
ORIGIN      
        1 aaagcccttg aaaatcgtga tgaacacgat cttcatgtcg aaccagaacg accagttatg
       61 gatgtagaag agatcgaact tgacgcgcgt ctccatcttt tcgaccttgg tggtcgcgcc
      121 gcgataaccg ttcacctggg cccagccggt aatgcccggc ttgatgcggt agcggttcat
      181 gtagccgtac acctgatcct tgtagagatc gtcgtgttcg accgcatgcg gacgcggacc
      241 gaccaccgac atctggccga gcagcacgtt cagaaattgc ggcagttcgt cgagactggt
      301 gcggcgcatg aagcggccga gtttcgtcac gcgcgcgtcg ttgcgcgcgg cctgcgtgag
      361 ttggccgtgc gcttcgtggt gcacggtcat ggaccggaac ttgtagatcg cgaacggctg
      421 gccgtccacg cccttgcggg tctggcggaa gaacaccggc cccggcgagc tgagcttgat
      481 gccgatcgcg agcgcgacga acacgggcgc gagcatgagc agcgcggccg ccgcgaacag
      541 gcggtcgaag atcagcttgg gccacatctg cggtgacgag aggggcgtcg cgctcagatt
      601 gagtgtcggt aaaccgacga catccaccag cgcgtggttg aagagcgaca ggctgcgcac
      661 atcgggtatg aagcgcagat tcacgaagtc atgcctgaag atgcgctgga agcgatagat
      721 cgtgtgttct tccgaaagcg gcaacgcgag ccacacttcg ttcacgtgct cttcgcgcac
      781 cttggccgcg aacgcattca gatcggtcag caccggcaag cgcttgagac gcgcgccgta
      841 cgtgccggcc gcttcgatgc tggtgtcgaa cacgcaaact ggtttgaagc ccgcttgcgg
      901 cgcatggtcg agatgcgcga gcagggtgcg cgagaagccg ggcgcaccga cgatcgcgac
      961 ggtgcgaaag ttcatgccgc gccggcgcac gctgcgcaag gccacgtgca cgaggcactt
     1021 ggtgacgatg atgagcgcgc cggagatcag cgtggagtaa ccgaaccaca gccgcgaaac
     1081 cgcatccatg cgatgcaagg tgaacgccag cacgagggcg gtggcgacca cgaccagcca
     1141 cgcggcggcc acgcgcgcca gcatggaagg cagcgccttg ccgcgccacg tttcatagac
     1201 gccgaagccg ggaaacatca acaacacgag cacgcagtta aacgcgatca ggaggcgctc
     1261 ggtgtcggac agcgccattg gtgccgagaa acgcatcccg tgagcgagca gcccgccagc
     1321 aatcacaaag agtgcgtcga ggcatcgcgc agtattcctg aacatgatga tctaccctgt
     1381 attcgaacga tgcagccttg aggccagcgg cttactcgac ggtttcagcc tggcgcagac
     1441 gcggcagcag acggtcgaat tcgcgcttca cgaggccgta gcattcgcag gcacgcgctt
     1501 ccagaccttt gcgatcgagg atcttgatgt ggccgcggct gtgatgaatc aggccttcgt
     1561 cgtgcaactt gccggccgct tcggtgatgc cttcgcggcg cacgccgagc atgtcggcga
     1621 tcagttgctg ggtaaccgtc agatcgttcg aggccacgcg atccacttcg atcagcaacc
     1681 agcggcacag ttgcttgttc aatgagtggt ggcgattgca ggccgcggtt tgcgcgactt
     1741 gcgtgagcag cgcgtgcata tacagcagca tcaaacggcg caggaaatcc gagcgggcga
     1801 attgctgctt gagcgcctgt gcgctcatcc ggtacgcgaa gccggcgcat tgcacctgta
     1861 cgcggttcgg catggtttcg ccaccggtga gcaccggcac gccggtcatg ccttcgcggc
     1921 cgaccgcggc gatctcgacc gagctgccgt cttccatcgt cgagagcatc gagatgatcg
     1981 cagtggtcgg gaagtagacg tgatggatcc gttgacctga atcgcacagc agttgttcgg
     2041 tgcgcagatg aaccagttcg agatgggggg c
//
LOCUS       DATASH010001097         6699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3949203,
            whole genome shotgun sequence.
ACCESSION   DATASH010001097 DATASH010000000
VERSION     DATASH010001097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6699
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3949203"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /gene="accB"
                     /locus_tag="VHV99_28970"
     CDS             <1..462
                     /gene="accB"
                     /locus_tag="VHV99_28970"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008342903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEX3639442.1"
                     /translation="LIDLVSESGISELEVTEGEGKVRIVKNAAPVYMQAPQYMPQGPM
                     QQAPQYGQPGDAGNGGAAAGAPATPAAVVPQGHVVTSPMVGSFYRAPSPGADAFVQVG
                     DTVKEGQTLCIIEAMKLLNEIESDKSGVVKEILVENGQAVEYGQPLFVIGD"
     gene            629..2011
                     /gene="accC"
                     /locus_tag="VHV99_28975"
     CDS             629..2011
                     /gene="accC"
                     /locus_tag="VHV99_28975"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017777083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX3639443.1"
                     /translation="MFEKILIANRGEIALRIQRACRELGVKTVIVYSEADKEAKYVKL
                     ADEAVCIGPAPSNLSYLNMPALISAAEVTDAEAIHPGYGFLSENADFAERVEQSGFVF
                     IGPRPETIRLMGDKVSAKQTMIKTGVPCVPGSEGALPEDPKEIVKIARAVGYPVIIKA
                     AGGGGGRGMRVVHTEAALVNAVNMTREEAGRAFGNPQVYMEKFLENPRHIEIQILADS
                     FRNAIWLGERDCSMQRRHQKVIEEAPAPGIARRLIERIGDRCADACKKMGYLGAGTFE
                     FLYENNEFYFIEMNTRVQVEHPVTELITGVDIVQEQIRIAAGEKLAFRQKDIVVRGHA
                     IECRINAEDPFKFTPSPGRLTSWHSPGGPGIRVDSHAYNGYFVPPNYDSMIGKLISYG
                     ATREQAINRMRIALSEMVVEGISTNIPLHREMMIDAKFVEGGTSIHYLENKLAARKAA
                     AAVAAEDAAQ"
     gene            2068..2970
                     /gene="prmA"
                     /locus_tag="VHV99_28980"
     CDS             2068..2970
                     /gene="prmA"
                     /locus_tag="VHV99_28980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX3639444.1"
                     /translation="MSYRELIAELDREHAEALSDALLDLGALSVSVEDADADTPDEQP
                     LFGEPGLTPDQKAWNRSRVVALLADDVDPAVLLAAAANEIGLKDTPAFTLRDVEDQDW
                     VRLTQSQFDPIPVGKRIWVVPSWHAAPDPDALVLELDPGLAFGTGSHPTTRLCMEWLE
                     QSVEQNQSVLDYGCGSGILAILAKKCGANPVIGIDIDPQAVESARQNSERNHADVTYG
                     LPADLPEGQFDIVVANILSNPLKLMASMLSARVKPGGRIALSGILARQADEVAAVYAR
                     WIDISVWREHEGWVCLAGTRRESL"
     gene            3016..4344
                     /locus_tag="VHV99_28985"
     CDS             3016..4344
                     /locus_tag="VHV99_28985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008342908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon and DUF3426 domain-containing
                     protein"
                     /protein_id="HEX3639445.1"
                     /translation="MALATRCPHCETVFRLDPHLLAPHDGRVRCGHCQEIFDAAHYQF
                     DRDETVMAALAATAPNYGVDPASPWAEKSSAEVSGDKRDALGIPLHPFVPKTALAPQV
                     EAEPTPAERAEPLAGFAAAEAVSRTRAQSDVPGEGRTESPWQAVNRAEADAQAQARAD
                     APARTEPRADSFVGRNEGDSLKESRTDPFIGRTENETLNESRAASRTEPFVGRSTPQA
                     EPETPAPRRPVDTTTRSEPGPHGAASAAPWAADPAEPSFGSAAASSGRASEPFPVIRE
                     TRAPARSGIVWTIIGSLIALVLLVTLIAQVAWWQRETVMVYWPASQRLFAQACEQIGC
                     VVAPPRDIDGLQIENSGLRQVDGSHKLELKLSLRNRFDVAIAYPALELTLLDDKNNVA
                     IRRVLWPRDYARPGTVIGAGLAARSTQPVIVRLDTGDAVAANYRVQIFYP"
     gene            4533..5033
                     /gene="tpx"
                     /locus_tag="VHV99_28990"
     CDS             4533..5033
                     /gene="tpx"
                     /locus_tag="VHV99_28990"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006765230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEX3639446.1"
                     /translation="MSQVTLGGNPIDVAGTFPAAGAKAVDFTLVGKDLGNLTLASFAG
                     KRKVLNIVPSLDTPTCATSTRKFNVAAAGLDNTVVVVVSGDLPFAATRFCTTEGIENV
                     VTASTFRGHDFAKAYGVDVTSGPLTGLTARAVVVIDENDKVLYSELVPEIKNEPNYDA
                     ALAALK"
     gene            5357..6292
                     /locus_tag="VHV99_28995"
     CDS             5357..6292
                     /locus_tag="VHV99_28995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016346276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEX3639447.1"
                     /translation="MSTLICGSLAYDNIMTFQGRFGDHILPDQVHQLNISFLVPTMRR
                     NFGGCAGNIAYALNLLGGDAKIMATLGEADAQLYLDRFDALGLSKEYVRVLPDQHSAQ
                     CFITTDLGNNQITAFHPGAMMDSHLNHAGETKGITLGIVAPDGADGMRQHVEELAAAG
                     VPFVFDPGQGLPILEGVELRRMIELATYVAVNDYEAKLLSNKTGWSIDEMLSRVQALV
                     ITRGEHGAQILHAEGIEDIPAVTAQKVVDPTGCGDAFRGGLLYGIENKLGWAKTGRLA
                     SLMGSLKIEHQGPQTYAPSRAEIGERFELAFGSAL"
     gene            6440..>6699
                     /locus_tag="VHV99_29000"
     CDS             6440..>6699
                     /locus_tag="VHV99_29000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639448.1"
                     /translation="MKMTSRMALAAALVSSVAVTGCAYNSSSPDVYTASQAQREETVR
                     MGTVDSVRGVKISTNNGQPSGLGAIGGGALGAVAGSALGGGRG"
BASE COUNT         1331 a         2065 c         2074 g         1229 t
ORIGIN      
        1 cttatcgacc tagtctcgga atccggcatc tcggagctgg aagttaccga aggcgaaggc
       61 aaggttcgca tcgtcaagaa tgccgcgccg gtctacatgc aggctccgca gtacatgccg
      121 caaggcccga tgcaacaagc accgcaatac ggccagccgg gcgatgcagg caacggcggc
      181 gccgcagccg gcgcgccggc aacgccggca gccgtcgtgc cgcagggcca cgtagtgacc
      241 tcgccgatgg tcggctcgtt ctaccgcgcg ccgtcgccgg gcgccgacgc gttcgtgcag
      301 gttggcgaca cggtcaagga aggccagacg ctgtgcatta tcgaagcaat gaagctgctc
      361 aatgaaatcg agtcggacaa gtcgggcgtt gtgaaggaaa tcctggtcga gaacggccag
      421 gccgtggaat acggtcagcc gttgttcgtg atcggcgatt gatttcccgc gattccgccg
      481 gacaagagcg cgttcagttg tgatgcgcct ggccggtggc cacagcactc gggcactcgc
      541 ctgaataacc ggcgagacgc gccgccgatc tcccaggacc gccgcgggca accgtcccgg
      601 cgttccatcg atgagtcgaa accttgctat gtttgaaaaa atcctcattg cgaaccgtgg
      661 tgaaatcgcg ctccgaattc agcgcgcgtg ccgcgaacta ggcgtcaaga cggtcatcgt
      721 ctattcggaa gccgataaag aagccaagta cgtgaagttg gccgacgaag ccgtgtgtat
      781 cggaccggct ccgtccaacc tcagctacct gaacatgccg gcgctgatca gcgcggccga
      841 agtcaccgac gccgaagcga tccatcccgg ctacggtttc ttgtcggaga acgcggattt
      901 tgcggagcgc gtcgagcaat ccggcttcgt gttcatcgga ccgcgtccgg aaaccatccg
      961 tttgatgggc gacaaagtct ccgccaagca aacgatgatc aagacgggcg tgccgtgcgt
     1021 gccgggttcg gagggcgcgt tacctgaaga tccgaaggaa atcgtgaaga ttgcacgcgc
     1081 cgtgggttat ccggtgatca tcaaggcagc gggcggtggc ggcgggcgcg gcatgcgcgt
     1141 ggtccacacc gaagcggcgt tggtgaacgc ggtgaacatg acccgcgaag aagccggccg
     1201 tgcattcggg aatccgcagg tctacatgga gaaattcctg gagaacccgc ggcacattga
     1261 aatccagatt ctcgcggatt cattccgtaa cgcaatctgg ctgggcgaac gcgattgctc
     1321 catgcagcgt cgtcaccaga aggtgattga agaagcgccg gcgccgggca ttgcgcgccg
     1381 cttgatcgag cgcattgggg accgttgcgc cgatgcctgc aagaagatgg gttatctcgg
     1441 cgcgggtacg ttcgaattcc tgtacgagaa caacgagttc tatttcatcg aaatgaacac
     1501 gcgcgtgcag gtcgagcatc cggtgacaga attgatcacc ggtgtggata tcgtccagga
     1561 acagattcgc attgctgccg gcgaaaagct cgcgttccgc cagaaggaca tcgtggttcg
     1621 cggtcacgcg atcgaatgcc ggatcaacgc ggaagatccg ttcaagttca cgccgtcgcc
     1681 gggacgtctc acgtcgtggc actcgcccgg cggtccggga attcgtgtcg attcgcatgc
     1741 gtacaacggc tatttcgtgc cgccgaacta cgactcgatg atcggcaagc tgatctccta
     1801 cggcgcgacg cgcgagcaag caatcaaccg catgcgtatt gcgttgtccg aaatggtcgt
     1861 ggaaggcatt tcgaccaaca tcccgctgca tcgcgagatg atgatcgacg cgaagttcgt
     1921 cgaaggcggc acgagcattc actacctcga aaacaagctc gccgcgcgca aggcggcggc
     1981 tgccgtcgct gcggaagacg cggcgcaata acgcaataac gcaacgacgc cataacgcca
     2041 taacgtaata agcagggttt caagaagatg agctaccggg aattgatcgc cgagctggac
     2101 cgggaacacg cggaagcgtt gtcggatgcg ctgctggatt tgggtgcgtt gtcggtatcg
     2161 gtggaagacg ccgacgccga tacccccgac gagcagccat tgttcggcga gcccggcctc
     2221 acgccggatc aaaaagcgtg gaaccgctcg cgggtcgtcg cccttctggc cgatgacgtc
     2281 gaccccgccg tgttgctcgc ggccgcggcc aatgagatcg gcttgaaaga cacgccggcc
     2341 ttcacgttgc gcgatgtcga agatcaggac tgggtgcgcc tcacgcaatc gcagttcgat
     2401 cctattcctg tcggcaagcg aatttgggtc gtgccgtcgt ggcatgcagc acccgatcct
     2461 gacgcgctcg tgctcgaact tgatccaggt ctcgcgttcg gtaccggcag ccatccgact
     2521 acccggctgt gcatggaatg gctcgagcaa tcggttgaac aaaaccagtc cgtgctcgat
     2581 tacggttgcg gttccggcat cctcgcgatc ctggcgaaga aatgcggtgc gaacccggta
     2641 atcgggattg atatcgatcc gcaagccgtg gaatccgcgc gccagaatag cgagcgcaat
     2701 cacgccgatg tcacctacgg cctgcccgcc gatcttcccg aagggcaatt cgatatcgtg
     2761 gtcgcgaaca ttctgtcgaa cccgctcaag ctaatggcgt cgatgctttc agcgcgcgtg
     2821 aagccgggcg gacgcatcgc tttgtcgggc attctggccc gccaggccga cgaagtggcg
     2881 gccgtctatg cacgctggat cgacatcagc gtatggcggg aacacgaagg ctgggtgtgt
     2941 cttgccggta cgcgccgcga aagcctttag aatagcgcca tcgtccatct accggcctct
     3001 ggccttcggc tacatatggc gctggcaacc cgctgtcccc actgcgaaac agttttccgt
     3061 ctggacccgc atttgctcgc gccgcacgac ggccgcgtgc gttgcggtca ttgtcaggaa
     3121 atcttcgacg cagctcatta ccagttcgac cgcgacgaaa ccgtcatggc cgcgctggct
     3181 gcaaccgcgc ctaactacgg cgtagatccg gcaagcccgt gggcagagaa gtcgagtgcg
     3241 gaggtctccg gcgacaagcg tgacgcgctg ggcatccctc ttcacccgtt cgtccccaaa
     3301 accgccctcg cccctcaggt cgaagcagaa cccacaccgg ctgagcgcgc cgaaccgctc
     3361 gcggggttcg ctgccgctga agcggtttcg cgcacgcgtg cgcagagcga cgtcccaggt
     3421 gaaggccgca ccgaatcccc gtggcaagcg gttaatcgcg ctgaggccga cgcacaagct
     3481 caagcacgcg ccgacgcccc cgctcgcacc gaaccgcgcg ccgattcttt cgtgggtcgt
     3541 aacgaaggcg attcgctgaa ggaatcgcgc actgatccct ttattggccg cactgaaaac
     3601 gaaacgctca atgagtcacg tgccgcgtca cgcacagaac cgttcgtcgg ccggagcaca
     3661 ccgcaagcag agccggaaac gcccgcgccc cgccgtccag tcgataccac cacccgttcc
     3721 gaacccggcc cgcacggcgc cgcatccgct gcgccgtggg cggcagaccc cgccgagccg
     3781 agcttcggca gcgcggctgc atcgtctgga cgcgcttctg agccgttccc cgttatccgt
     3841 gaaacgcgcg cgccggcccg ttcgggaatc gtttggacga tcatcggttc gctgatcgcg
     3901 ctggtgctgc ttgtcacgct gatcgcgcag gtcgcatggt ggcaacggga gaccgtgatg
     3961 gtctactggc ccgcctcgca gcggctcttc gcgcaagcct gcgaacagat tggttgcgtg
     4021 gtcgcgccgc cgcgggatat tgacggcctg cagatcgaga actcgggtct gcgccaggtg
     4081 gacggatcgc acaagcttga actgaaactc tcgctacgca atcgcttcga cgtggcgatc
     4141 gcctatccgg cgctggagct caccctcctc gacgacaaga acaacgtcgc gatccggcgt
     4201 gtcctgtggc cgcgagacta cgcgcgtccg ggcacggtga tcggcgcagg tcttgccgcc
     4261 cgcagtacgc agccggtgat cgtccggctc gatacgggcg acgcggtcgc ggcgaattat
     4321 cgtgtgcaga tcttttatcc ctgatcggcg tctgcttcaa cgcgtcagct ttcacgtttt
     4381 tacgcttgac cgccttcgat gcgtttttcc gcgcgtttct ataattagcc agtcgtttat
     4441 tagccagtcg ttttttccgc gcgcgtcgct cgtccgtccc cggtcgaacg acgcgcatat
     4501 tccctgaaca tcagacactt tctggagtag acatgagcca agttaccctc ggcggcaacc
     4561 cgatcgacgt ggcaggcacg ttcccggcag ccggtgcaaa agcagtcgat ttcaccctgg
     4621 tcggcaagga tctgggcaac ctgacactgg cgagcttcgc cggcaagcgc aaggttctga
     4681 atatcgtgcc gagcctcgac accccgacgt gcgccacctc cacacgcaag ttcaacgtcg
     4741 ccgcagccgg cctcgacaac accgtggtcg tggttgtgtc gggcgatctg ccgttcgcgg
     4801 caacgcgctt ctgcacgacg gaaggcatcg agaacgttgt gacggcatcg acgttccgcg
     4861 gccatgactt cgcgaaggcc tacggcgtcg acgtcacgag cggaccgctg acgggcctga
     4921 cggcgcgcgc agtggtcgtc atcgatgaaa acgacaaggt gctgtactcg gagctcgtgc
     4981 cggaaatcaa gaacgaaccg aactacgacg cagcgctggc cgcgttgaag taagcattga
     5041 agaacgcggg ccggttgcgg cctgcctctc tccgggcggc ggcgagccgc atctctatcc
     5101 tgctctgggt gcacccctcg ccgcgccgga taaacagccg aagcaagaca gttattcagt
     5161 cagccgtggc ggcttcaacc tgccacggcc gctaccggca atgcgcatcc gtgactgggt
     5221 ggaacacccg ccgcgtgacg aatgcgcagc gtttcatgca ggcatgcatg gtgcacagga
     5281 actcaagcct gcgcgcactt tcatctacgc cattcgatcg acgcattcga tcaatttaat
     5341 ttcaggaaat tcgccagtgt ctacgctcat ctgcggctcg ctcgcctacg acaacatcat
     5401 gactttccag ggccgcttcg gcgaccacat cctgccggac caggtgcatc aactgaacat
     5461 cagcttcctc gtccccacga tgcggcgcaa tttcggcggc tgcgcgggca acattgcgta
     5521 cgcgctgaac ctgctcggcg gcgacgcaaa gatcatggcg accctcggcg aagccgacgc
     5581 ccagctttat ctcgatcgtt tcgatgcact cggtttgtcg aaggaatatg tgcgcgtcct
     5641 gcccgatcag cactcggcgc aatgcttcat caccaccgat ctcggcaaca accagatcac
     5701 cgcgttccac ccgggcgcga tgatggattc gcacctgaac cacgccggcg aaaccaaggg
     5761 catcacgctc ggcatcgttg caccggacgg tgctgacggc atgcgccagc acgtcgagga
     5821 actggcggca gcgggcgtgc ctttcgtctt cgatccaggc cagggcctgc cgattctcga
     5881 aggtgtggaa cttcgtcgca tgattgaact cgccacttac gtggcagtca atgactacga
     5941 ggcaaaattg ctgagcaaca agaccggctg gtcgatcgac gaaatgctga gccgcgtgca
     6001 agcgctcgtg attacgcgcg gcgaacacgg cgcgcagatt ttgcacgctg aaggtatcga
     6061 agacattccg gccgtgacgg cgcagaaagt cgtcgaccca acgggttgcg gcgacgcgtt
     6121 ccgcggtggt ttgctgtacg gcatcgagaa caagctgggg tgggcaaaaa cgggccgtct
     6181 cgccagtttg atgggctcgc tgaaaatcga acatcaggga ccgcagacgt atgcgccctc
     6241 ccgcgccgag atcggcgaac gcttcgagct ggcttttggt tcggcgctct agaggtttag
     6301 atcggtgcag gaaggttgaa atgagcaggg ttgaaatgag tcattgaagc ggatttgggc
     6361 gacgcttggg caatgacacg atgtaccagg ctgttggctt ttccgggcaa cagcctgccg
     6421 taatttagtg gagtgaagaa tgaagatgac aagtcgtatg gcgctggcag cagcgcttgt
     6481 tagctccgtc gccgtgacgg gatgcgccta taacagcagt tcgcccgatg tgtacaccgc
     6541 gtcgcaagcg cagcgcgaag aaacggtccg catgggtacc gtcgacagcg tgcgcggcgt
     6601 gaagatcagc acgaacaacg gccagcccag cgggcttggc gctatcggcg gcggcgcact
     6661 gggcgcagtg gcaggcagcg cgcttggcgg cgggcgtgg
//
LOCUS       DATASH010001098         3430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2168847,
            whole genome shotgun sequence.
ACCESSION   DATASH010001098 DATASH010000000
VERSION     DATASH010001098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2168847"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..128
                     /locus_tag="VHV99_29005"
     CDS             <1..128
                     /locus_tag="VHV99_29005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012400651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX3639449.1"
                     /translation="PQFMAVLDKLPRHVPRGIEFPFDANRIDADAAHYVAWLAGA"
     gene            478..1461
                     /locus_tag="VHV99_29010"
     CDS             478..1461
                     /locus_tag="VHV99_29010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEX3639450.1"
                     /translation="MSNTNAALDVITYGEAMAMFVAAETGALAGVGQFTKRVAGADLN
                     VAIGLSRLGFKVGWMSRVGRDSFGQYVRDTLTKEGIDQRCVTTDECYPTGFQLKSKND
                     DGSDPAVEYFRKGSAASHLSLADYVADYVLPARHLHLTGVAPAISASSRELAFHLARE
                     MRAAGKTISFDPNLRPTLWPSRAAMVEGLNALAALADWVLPGIGEGGILTGYTQPDDI
                     AKFYLDQGARGVVIKLGAQGAYFRTADDAAVIAGQPVAKVVDTVGAGDGFAVGVVSAL
                     LEGRTLPHAVARGNRIGAMAIQVIGDSEGLPSRAELDALERADVPSVTTAA"
     gene            1900..3192
                     /locus_tag="VHV99_29015"
     CDS             1900..3192
                     /locus_tag="VHV99_29015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3639451.1"
                     /translation="MTSSLAIRRWWTIMPIVFITYSLAYLDRANFGFASAAGINQDLG
                     ISKGLSSLIGALFFLGYFFFQIPGAIYAERRSVKKLVFWSLILWGGCAALTGMVSNIP
                     SLMVIRFVLGVVEAAVMPAMLIFISNWFTKSERSRANTFLILGNPVTVLWMSVVSGYL
                     VHSFGWRPMFIAEGVPAILWAVCWWFIVQDKPQQVSWLTQQQKDDLAGTLRAEQAAIK
                     PVRNYSEAFRTPAVIKLCAQYFCWSIGVYGFVLWLPSILKNGSTLGMVETGWLSALPY
                     LVATIAMLAASWASDKLNRRKVFVWPFLLVGAVAFAASYALGSTHFWISYALLVIAGG
                     AMYAPYGPFFAIVPELLPKNVAGGAMALINSMGALGSFVGSYVVGYLNGATGSPAASY
                     AFMSVALVAAVILTLIVKPQQAQPRTKAASLATPLQGK"
     gene            3197..>3430
                     /locus_tag="VHV99_29020"
     CDS             3197..>3430
                     /locus_tag="VHV99_29020"
                     /EC_number="1.1.1.79"
                     /EC_number="1.1.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051722.1"
                     /note="catalyzes the formation of glycolate from
                     glyoxylate and glycerate from hydroxypyruvate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glyoxylate/hydroxypyruvate
                     reductase B"
                     /protein_id="HEX3639452.1"
                     /translation="MKKIVAWKSLPKDVLAYLQQHAEVVQVDPAQRDTFVAALKDADG
                     GIGSSVKITPAMLEGASRLKALSTISVGFDQFDV"
BASE COUNT          691 a         1044 c         1096 g          599 t
ORIGIN      
        1 acccgcagtt catggccgtg ctcgacaagc tgccgcgcca cgtaccgcgc ggcattgaat
       61 ttccgttcga tgcaaatcgt atcgatgcgg acgcggcgca ctacgtcgcg tggctggccg
      121 gcgcgtaagc ggcgcgtagg acctgagaga gcagtactca aaaaaattgc gcggcaaaga
      181 ggctcgtctg gagaaggaag tacacggaaa tacgggcgga agcacacgca gagaagcgcg
      241 agcagcacgg cacatgcaag gcagcgtgcg caacgcggca caagcggcgc ggcacaagca
      301 gcacaccgga gcccgcagga gcgaagctag cgacacggca tatgcaaggc agcgtgcaca
      361 acgcggctca agcaaccgca gcacgagcaa cgcagcacga gcaacgcagc gcaagcaacg
      421 cagcacgagc agcaaaaagc cgcacaagca gctcaccgga acccgcagga gcgaagcatg
      481 agcaacacaa acgcagcact cgacgtcatc acctacggcg aagcgatggc catgttcgtg
      541 gctgccgaaa ccggcgcgct cgccggcgtc gggcagttca ccaagcgcgt cgcgggcgcc
      601 gatctgaacg tcgcgatcgg cctgtcgcga ctgggtttca aggtgggctg gatgagccgc
      661 gtcggccgcg attcattcgg ccagtacgtg cgcgacacgc tgacgaaaga aggcatcgac
      721 cagcgctgcg tcaccaccga cgagtgctac ccgaccggct ttcaactgaa gtcgaagaac
      781 gacgacggca gcgatccggc cgtcgagtac ttccgcaaag gctcggcggc gagccacctg
      841 tcgttggccg actacgtcgc cgattacgtg ctgccggcgc gtcacttgca tctgaccggc
      901 gtggcgccgg cgatctcggc cagctcgcgc gaactcgcgt tccatctggc gcgagaaatg
      961 cgcgctgccg gcaagacgat ttcgttcgac ccgaatctgc gcccgacgct gtggccctcg
     1021 cgcgccgcga tggtcgaagg cttgaacgcc ctcgccgcat tggccgactg ggtgctgccc
     1081 ggcatcggcg aaggcgggat cctgaccggc tacacgcagc cggacgacat cgcaaagttt
     1141 tacctggacc agggcgcgcg cggcgtggtc atcaagctcg gtgcgcaagg cgcgtatttc
     1201 cgcaccgccg acgatgccgc cgtgatcgcc ggccagccgg tcgcaaaggt ggtggatacg
     1261 gtcggcgcag gcgatggctt tgccgtcggc gtggtcagcg cgttgctcga aggcaggacg
     1321 ctgccgcacg cggtggcgcg tggcaaccgc atcggcgcaa tggcgatcca ggtgatcggc
     1381 gattcggaag gcctgccgag tcgcgcggaa ctcgacgcgc tcgaacgcgc cgacgtgcca
     1441 tcggttacca cggcagcctg agcgcgtgca cgccgtgccg aacgccgtat tcagcgcatc
     1501 cggcgcaagt aacgcaagta acgcaagtaa cgcaagcagc acagataagg caagcagcac
     1561 agataaggca agcagcacag ataaggcaag cagcacagat aaggcaagca gcacagataa
     1621 ggcaagcagc accagtacgc aagcagcagc ccatcagctt cggccagttc gataacagca
     1681 agagcgctcc aacagcgccc catgaccgcc catcattgct tcgggaagcc gttagcaact
     1741 atcttgaatg ggaccaaagt aagtgctgtg catgggacca gagtaagtgc tttgcatggg
     1801 accaaagtaa gcgctaaagc gctaactctg gtcgacagct aaagcaccaa ctccggtcga
     1861 cagctaaagc accaactctg gtcgacaaag gagactccca tgacctcatc gctcgcgatt
     1921 cgccgttggt ggacgatcat gccgatcgta ttcatcacgt acagcctcgc ctatctcgac
     1981 cgtgcaaact tcggtttcgc gtcggccgcc ggtatcaatc aggatctcgg catcagcaaa
     2041 ggcttgtcgt cgttgatcgg cgcattgttc ttcctcggct atttcttctt tcagattccc
     2101 ggcgcgattt acgcggaacg ccgcagcgtg aaaaagctcg tgttctggag cctgattctg
     2161 tggggcggct gcgcggcgct gaccggcatg gtcagtaata tcccatcgct gatggtgatc
     2221 cgcttcgtgc tcggtgtggt cgaagccgca gtgatgccgg cgatgctgat ttttatcagc
     2281 aactggttca ccaaaagcga gcgctcgcgc gccaacacct tcctgattct cggcaatccg
     2341 gtgacggtgc tgtggatgtc ggtggtgtcg ggctatctgg tgcactcgtt tggctggcgt
     2401 cccatgttca tcgccgaagg cgtgcccgcg atcctgtggg cggtgtgctg gtggttcatt
     2461 gtgcaggaca agccgcagca ggtgtcgtgg ctgacgcagc agcagaagga cgatctcgcc
     2521 gggacgttgc gcgcggaaca ggccgccatc aaaccggtgc gcaattacag cgaagcattc
     2581 cgcacgcccg ccgtgatcaa attgtgcgcg caatattttt gctggagcat cggcgtgtat
     2641 ggtttcgtac tgtggctgcc gtcgatcctg aagaacggtt cgacgctcgg catggtcgaa
     2701 accggctggc tctcggcgct gccgtacctg gtcgcgacga tcgccatgct tgcggcttcg
     2761 tgggcgtcgg ataaactcaa ccgccgcaaa gtgttcgtgt ggccgtttct gctggtcggc
     2821 gcggtggcgt tcgccgcctc gtacgcgctc ggctccacgc acttctggat ctcgtacgcg
     2881 ctgctggtga tcgccggcgg tgcgatgtat gcgccttacg gcccgttctt cgcgatcgtg
     2941 ccggagctcc tgccgaagaa cgtcgcgggc ggcgcgatgg cgctgatcaa cagcatgggc
     3001 gcgctcggtt cgttcgtcgg ctcgtatgtg gtcggctatc tgaacggcgc gaccggctcg
     3061 cccgcggcat cgtatgcgtt catgagcgtg gcgttggtcg ccgcggtgat tctgacgctg
     3121 atcgtcaagc cgcaacaggc gcagccgcga acgaaggccg cctcactcgc tacaccgttg
     3181 caaggaaaat gaactgatga agaagatcgt cgcctggaag tcgttgccca aggatgtgct
     3241 cgcgtatctg caacagcatg cggaagtcgt gcaggtcgac cccgcgcaac gtgacacgtt
     3301 cgtcgccgcc ttgaaggatg cggacggcgg catcggttcg agcgtgaaaa tcacgccggc
     3361 gatgctcgaa ggcgcgagcc ggctgaaggc gctgtcgacc atctcggtgg gcttcgatca
     3421 gttcgacgtt
//
LOCUS       DATASH010001099         5448 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1775527,
            whole genome shotgun sequence.
ACCESSION   DATASH010001099 DATASH010000000
VERSION     DATASH010001099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5448
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1775527"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..380)
                     /locus_tag="VHV99_29025"
     CDS             complement(189..380)
                     /locus_tag="VHV99_29025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639453.1"
                     /translation="MANSESRLQGSGSTIIFSACDLPMTGLTGPMRDRIEAPHLLNRY
                     AKSARDNAAARQFEDAGRA"
     gene            637..3090
                     /locus_tag="VHV99_29030"
     CDS             637..3090
                     /locus_tag="VHV99_29030"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HEX3639454.1"
                     /translation="MTAVDLEFDQLNSTVDALRRSISNRMMYGVGKDAVTAHPHDWLH
                     AAALAVRDRLVARWMKTTRLQYEQDVKRVYYLSMEFLIGRTFTNALLALGIHDQMKEA
                     LASLGVDMEMLVNIEPDAALGNGGLGRLAACFLDSMATLGIPGFGYGIRYEYGMFRQE
                     IVDGEQVEAPDYWLRAGNPWEFPRPEIKYTVHFGGRTVQRGEHIEWIDTQHVNATAYD
                     TVIPGYATSATNTLRLWSARATEELDLGAFNRGDYRNAVDTKNMSENVSRLLYPDDST
                     PAGRELRLRQEYFFVSATMQDLIRRYQRTHSTFGRFSEKVAVHLNDTHPVLAIPELMR
                     LLVDVHHQPWDKAWQHVTKIFSYTNHTLMPEALETWDVEMLARLLPRHLEIIFEINAG
                     FLKHVSEQSGHDAEMIRRISLVDEYGQRRVRMAYLAIVASHKVNGVSKLHSQLMVRDI
                     FADFARIYPDRFTNVTNGITPRRWLAQASPSLSSLIDQQIGKHWRSNLFELEQLRGLR
                     NDSGFIDAFREAKRQNKVRLVQRLAHHTKLHFDPDALFDLQVKRIHEYKRQLLNVLHV
                     IVRYNQIRANPERDWVPRVVMFAGKAASAYRMAKTIIKLIGDVSEKVNHDPLVGDRLK
                     VVFVPNYGVSVAELIIPAADLSEQISMAGTEASGTGNMKLALNGALTIGTMDGANIEI
                     CDAVGRENIFIFGHTADEVDSLRATGYRPRQVYEENPELRMALDQIRTGFFSPDDPLR
                     FSDIFHTLVDWGDHYMVLADFAAFAKAQDEVDARFVDQRAWAESAIENVAGMGQFSSD
                     RTIGEYARNIWHVNPLSMD"
     gene            complement(3178..4269)
                     /locus_tag="VHV99_29035"
     CDS             complement(3178..4269)
                     /locus_tag="VHV99_29035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639455.1"
                     /translation="MFLSSLDAARRRFHHCVRHSTAAALVAGGALVALTGCSTTFPTP
                     TPPAPNAALINGGNDGLLIPLHVERADEDHARLGLPVQLDGKPVYMALDTGTQGARVL
                     KSVLPGSNYASAGGVTSLSFANGVEILGSGVKVPLSVAGTTPTPVAAQAVDVVRCLPN
                     ARRCAGMDGYTGEFGWAFSGIFGVGAAQPDDNTYTQPLRALPGNIGQRYLVHSNFAKP
                     YMVLSPSNAITKDYTMVPMTLSKDGTAQWPRGCVNVGNKMTFCAPLEFATGSTGMIRI
                     ETDKAPNWVGDGDVGNVLDQGNYETAIGTGPWVHRYETAQVTIVKAKPDANRIVVGLM
                     AMQNIDILFDFPRAQFGLHAAKESERFAP"
     gene            complement(4532..4876)
                     /locus_tag="VHV99_29040"
     CDS             complement(4532..4876)
                     /locus_tag="VHV99_29040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639456.1"
                     /translation="MMLTSVATSEISASRARRESSLPRRTHVVVPLVIIDVTVPGTSS
                     ANGRRALHTALGDDLRLYVVTIDQQHERVTFRVEVTSRSLDEVIGLLTATLDHATLGR
                     AQVTVIRRPRNY"
     gene            complement(5022..5411)
                     /locus_tag="VHV99_29045"
     CDS             complement(5022..5411)
                     /locus_tag="VHV99_29045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6232 family protein"
                     /protein_id="HEX3639457.1"
                     /translation="MDTPFNDRGVSVTRNALSSAGQVFPLRDIVDVQVVTVQKNKVLP
                     RAISLVGLIGAIVGGIFRSPAGMVCGTMLVVVGWLTWATQDVTHRLMVQTANGMREAL
                     MSTDREFVERVAAAVHEAKAAPAAPRA"
BASE COUNT         1037 a         1643 c         1754 g         1014 t
ORIGIN      
        1 cggacgcgtt ggcgggacag cgcggcgtgc aagcggcagg gttgcgtggc gtgaaacggg
       61 cgcggcctga gcccggcggt cagggtggct cgcgcggcga atggtgcgat tgttacagag
      121 accaggaaat cgtgctggaa ggcagccggg cgagggcgcc tggttaaagg ccatgatgca
      181 actaacggtc aagcccgccc ggcatcttcg aactggcgcg cggccgcgtt atcacgcgcc
      241 gatttggcat aacggttcag cagatgaggt gcttcgattc gatcacgcat cggacctgtc
      301 aatccagtca tcggcaagtc acacgccgaa aaaataatcg tgctaccgct cccttgcagg
      361 cggctttcac tgttcgccat cgttcgcctg tttggcgagc cgatgcacgc gcgcagtgcc
      421 gtcacctgcc gcttgcaccg cgcacgtgcg caaaaccggc caggcgccgc gtgtcggcgg
      481 gcatggcatg tgcgtgagca cccgctcgcg cccgccggtg tccgcgggcc gtaaaatgtc
      541 gtactctgtt tgctcacccg cctgaccggc acgcgcctcc cgtctgtcat ctatgcggtt
      601 tgcgtatgtc gcgtcgtttt ttccccggag gcttcgatga cagccgtcga tctggaattc
      661 gaccagctca acagtaccgt cgacgcgcta cggcgctcaa tttccaatcg catgatgtac
      721 ggcgtcggca aggacgccgt caccgcgcac ccgcatgact ggctgcacgc cgcggcgctc
      781 gccgtgcgcg accggctggt tgcacgctgg atgaagacca cgcgcctgca gtacgaacag
      841 gacgtcaaac gcgtctacta cctgtcgatg gaattcctga tcggccgcac gtttacgaac
      901 gcattgcttg cgctcggcat ccacgatcag atgaaggaag cgctcgcgag tctcggcgtc
      961 gacatggaga tgctggtcaa tatcgagccg gatgcggcac ttggcaacgg cggcctcggc
     1021 cggctcgcgg cctgttttct cgattcgatg gcgacgctcg gcattccggg ttttggctac
     1081 ggtatccgtt atgaatacgg catgtttcgc caggagatcg tcgacggcga gcaggtcgaa
     1141 gcaccggact actggctgcg cgcgggcaat ccgtgggagt ttccgcggcc cgagatcaag
     1201 tacacggtgc atttcggcgg ccgcacggtg cagcgtggcg agcacatcga gtggatcgat
     1261 acccagcatg tcaacgccac ggcctacgac accgtgattc cgggctacgc gaccagtgcg
     1321 acgaacacgc tgcggctgtg gtcagcgcgc gccaccgaag aactcgacct cggcgcgttc
     1381 aaccgcggcg attaccgcaa tgcggtcgac accaagaaca tgtcggagaa cgtgtcgcgg
     1441 ctgctctatc cggacgattc gacgccggcc ggccgcgaat tgcggctgcg ccaggaatac
     1501 ttctttgttt cagcgacgat gcaggatctg attcgccgct atcagcgcac gcacagcacc
     1561 tttggacgct tctccgaaaa ggtcgcggtg catctgaacg atacgcaccc ggtgctggcg
     1621 attccggagc tgatgcgttt gctggtggat gtgcatcatc agccgtggga taaggcatgg
     1681 cagcacgtca cgaagatctt ctcgtacacc aaccacacgt tgatgcctga agcgctcgaa
     1741 acgtgggatg tcgagatgct cgcgcgcctg ctgccgcggc atctggagat catcttcgag
     1801 atcaacgccg gttttctcaa gcatgtcagc gagcaatcgg gccatgacgc cgagatgatc
     1861 cgccgcattt cgctcgtcga cgagtacggc cagcggcgtg tgcggatggc gtatctggcg
     1921 attgtcgcga gtcataaggt gaatggcgtc tccaagcttc attcgcaatt gatggttcgc
     1981 gatatctttg ccgacttcgc gcgcatctat ccggatcgct tcaccaacgt gaccaacggc
     2041 atcacgccgc ggcgctggct cgcgcaggcg agtccgtcgc tgtcgtcgct gatcgatcag
     2101 cagatcggca agcattggcg cagcaatctg ttcgagctgg aacaactgcg cggtctgcgc
     2161 aacgacagcg ggttcatcga cgctttccgc gaagcgaagc ggcagaataa agtgcgcctc
     2221 gtccagcggc tcgcgcatca cacgaaactg cacttcgatc ccgacgcgtt gttcgacctg
     2281 caggtcaagc gcattcacga atacaagcgg cagttgctga acgtgctgca cgtgatcgtg
     2341 cgctataacc agattcgcgc gaatccggag cgtgactggg tgccgcgggt ggtgatgttc
     2401 gccggcaagg ccgcgtccgc gtatcgcatg gcgaagacga tcatcaagct gatcggcgac
     2461 gtcagcgaga aagtcaatca cgaccccttg gtgggcgatc gcctcaaagt ggtgttcgtg
     2521 ccgaactacg gcgtgagcgt ggccgagttg atcattccgg ccgccgatct atccgagcag
     2581 atttcgatgg ccggcaccga agcgtcaggc accggcaaca tgaagctcgc gctgaacggc
     2641 gcgttgacga tcggcacgat ggacggcgcg aacatcgaga tctgcgacgc agtggggcgc
     2701 gagaatatct tcatcttcgg ccataccgcc gacgaagtgg acagcctgcg cgccaccggc
     2761 taccggccgc gccaggtgta cgaggagaat ccggaactgc gcatggcgct cgaccagatt
     2821 cgcacaggct tcttctcgcc ggacgatccg ctgcgtttct cggacatctt ccatacgctg
     2881 gtggattggg gcgatcacta tatggtgctc gccgatttcg ccgcgtttgc gaaagcgcag
     2941 gacgaggtgg atgcgcgttt cgtcgaccag cgcgcgtggg ccgagagtgc gatcgagaat
     3001 gtcgcgggca tggggcagtt ttcttcggac cgcaccatcg gcgaatatgc gcgcaacatc
     3061 tggcacgtga atccgctcag tatggactga cgcaggcgcg ttcgctgatg gcgtctgctc
     3121 aaataaaaag gccgtgcggg attttttcgc acggcctttt ctatcgactg gtgaacttca
     3181 cggggcgaat cgttcgctct ctttcgccgc gtgcaaaccg aattgcgcgc gcgggaagtc
     3241 gaacaggatg tcgatgttct gcatcgccat caaaccgacc acgatgcggt tagcgtccgg
     3301 cttggccttg acgatcgtaa cctgagcggt ctcgtaacgg tgcacccatg ggcccgtgcc
     3361 gatcgctgtc tcatagttgc cctgatcgag cacattgccg acatcgccgt caccgaccca
     3421 gttgggcgct ttgtccgttt cgatgcggat catgccggtg ctgccggttg cgaactccag
     3481 cggcgcgcaa aaggtcatct tgttgccgac attcacgcag ccgcgcggcc attgcgccgt
     3541 gccgtctttc gacagcgtca tcggcaccat cgtgtagtcc ttcgtgatgg cgttcgacgg
     3601 gctcagcacc atgtacggct tcgcgaagtt cgagtgcacc agataacgtt ggccgatgtt
     3661 gccgggcaac gcgcgcagcg gttgcgtgta ggtgttgtcg tccggctgcg cggcgccgac
     3721 gccgaaaata ccggagaacg cccagccgaa ttcaccggtg tagccgtcca tcccggcaca
     3781 gcgccgcgcg ttcggcaggc accgcacgac gtccaccgct tgcgcggcga cgggcgttgg
     3841 cgtggtgccg gcaacgctaa gcggcacctt gacgcctgaa ccgagaatct ccacgccgtt
     3901 cgcgaatgac aacgacgtca cgccgccggc gcttgcatag ttcgaacccg gcaacaccga
     3961 cttcaacacc cgcgcacctt gcgttcccgt gtcgagcgcc atgtaaaccg gtttgccgtc
     4021 gagctggacc ggcaggccga ggcgtgcatg atcttcgtcg gcacgttcca cgtgcagcgg
     4081 aatcagcagg ccgtcgttgc cgccgttgat cagcgcggca ttgggggccg gcggcgtggg
     4141 ggtggggaag gtggtggagc agccggtcag cgcaacgagc gcgccgccgg cgaccagtgc
     4201 cgccgcggtt gaatgacgaa cacaatgatg aaaacgccgg cgcgcggcat cgagagacga
     4261 caggaacata agatcacgct ttgcaaaaag gcgggtccct ggcgaaggcg atgcatcgtg
     4321 agctgcaaat tggacagcct ggacagcact ggcggcaatc tcactggatt gccgcagcgt
     4381 ggacggggaa accgggacgc ttggggggcc tgcgggaggg aacacgcgat aacaggagcg
     4441 gcgactctac acggccaccc gcaggcaatt gcaaaaatgt gtcaaggttt gagtgtggct
     4501 agctacttgc ccgtgcaagc attgatctat gtcaatagtt gcgcgggcgg cggatcacgg
     4561 tgacttgcgc gcggccgagc gtggcatggt cgagagtggc cgtcagcagg ccgatcactt
     4621 cgtcgagcga gcgcgaggtg acttcaacac gaaaagtgac gcgttcgtgc tgctggtcga
     4681 tagtgacgac gtaaagacgc agatcgtctc cgagggcggt atgcaatgcg cgccgcccat
     4741 tggccgacga agtgccgggc acggtgacgt cgatgatgac gagcggaacg acgacatgcg
     4801 tgcggcgggg aagacttgat tcgcgccgtg cgcgcgaggc ggagatttca gacgttgcta
     4861 cggacgtgag catcatgatt gcgccgattc ggcgaggcgg cgcgcgttgt tgacacccaa
     4921 ccactctagc ggcgcgcgcc tcaacgacgc gcaaaagctg ccgcctggcg aatcaaaatt
     4981 gtgttaacgc gcgatgcctc gcatgtgtgc acggcgcccg ctcaagccct gggcgccgcg
     5041 ggagctgcct tcgcctcgtg cacagcggcg gcgacgcgct cgacgaactc gcgatcggtg
     5101 ctcatgagcg cttcgcgcat gccattggcg gtctgcacca tcaggcgatg cgtgacgtcc
     5161 tgagtcgccc acgtgagcca gccgaccact accagcatcg tgccgcacac catgccggcc
     5221 ggcgagcgga atatcccgcc gacaatggcg ccgatgagcc cgacgagcga gattgcacgt
     5281 ggcaagactt tgttcttctg cacggtgacg acttgcacgt cgacgatatc gcgcagcgga
     5341 aaaacctggc cggcggacga gagtgcgtta cgcgtgaccg acacgccgcg atcgttgaaa
     5401 ggagtgtcca tcgatttcaa tgcgagggtg atgaaggccg cagcgtag
//
LOCUS       DATASH010001100        12292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4741921,
            whole genome shotgun sequence.
ACCESSION   DATASH010001100 DATASH010000000
VERSION     DATASH010001100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 12292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12292
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4741921"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VHV99_29050"
     CDS             complement(<1..358)
                     /locus_tag="VHV99_29050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEX3639458.1"
                     /translation="MALGAAGCTTPGVTNAPSSPATVSSSATLPSWRDGAAKQTIEKF
                     VADVTRAGSPNFDPPAGRIAVFDNDGTLWSEQPLYFQFAFMLDQVKAAAPKHPEWNNN
                     PAFKALVANDYAALASQ"
     gene            complement(349..564)
                     /locus_tag="VHV99_29055"
     CDS             complement(349..564)
                     /locus_tag="VHV99_29055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639459.1"
                     /translation="MCLYSGRYPGLHPGLHPGLYPGLYPGLYPGLHPGLHPGPHPGPH
                     PGQHSARHSARHSGRRSALLSSRSSWH"
     gene            complement(713..1834)
                     /locus_tag="VHV99_29060"
     CDS             complement(713..1834)
                     /locus_tag="VHV99_29060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEX3639460.1"
                     /translation="MLPACVGAAVSWAVTSHNQPHPQVVYGDGRHGPLGMAWIPGGQF
                     LMGSDSKQAQPNERPAHKVRVHGFWMDRHHVTNAEFRKFVEATGYVTTAEKKPDWDTL
                     KVQLPPGTPRPPDSAMVAGAMVFVGTNRPVPLEDYSQWWRYVPGANWRHPTGPDSNII
                     GKDDHPVVQVSYEDAQAYAKWAGKRLPTEAEWEFAARGGLEQATYAWGNQFAPNGKQM
                     ANVWQGQQVQSFPVVSPKAGGALGTSPVGTFPANGYGLSDMTGNAWQWVADWYRADQF
                     RREAVSTSTIDNPAGPRDSWDPADQGVPVDAPKRVTRGGSFLCNEDYCLSYRPSARRG
                     TDPYNSMSHLGFRLVMDEDTWKQPLARQASARAADADGS"
     gene            2226..3185
                     /locus_tag="VHV99_29065"
     CDS             2226..3185
                     /locus_tag="VHV99_29065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX3639461.1"
                     /translation="MRETILRFESSVNEAIVGQQAVVRQILIALLADGHVLLESLPGL
                     AKTRTVKAIATRLAATMSRIQFTPDLLPSDITGAETLLQSGAERTLSFQPGPIFGNLI
                     LADEINRAPAKVQSALLEAMEERQISVAGKTHKMSDLFLVMATQNPIEQEGTYPLPEA
                     QMDRFLLKVSMSYPSPQSEVDMLRLLRRESGEADNMPNVLAQEVIFAVREEIRRITVA
                     PAVDDYIVSLVNATRHGRELDAELGKWIEIGASPRGSIGLDRASRVQAWLQDRTFVTP
                     DDVRAVVHPVLRHRLILSYDANAENISADAVVDRLIEKVAVPA"
     gene            3330..4301
                     /locus_tag="VHV99_29070"
     CDS             3330..4301
                     /locus_tag="VHV99_29070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX3639462.1"
                     /translation="MSESASCAPSADGRVYVDAVHLAKLEFHARGLSFIAPAPVSSVL
                     SGSHASRLRGRGLNFEEIRAYLPGDDVRHIDWKVSLRLGKPQIRAYTEERDRPLLVVV
                     DQRMSMFFGSRRAFKSVVAAEVAALAVWMGFTAGDRVGGVLFDDSRIVRVRPLRSRSR
                     IKVLFGALAAMNRALHADSEARTDYAQLNAALEGVLQLAGHDYLICIVSDFAGADERT
                     QRLLRQLSAHNDVVAAMVFDPLWQHMPEHRALVVSEGHLQVELRIEQERVRAPLSSLF
                     SGRTAEVAELLRSSGVPLIALSTAEPTVEQVRRLFGQRARPATGSGL"
     gene            4298..4828
                     /locus_tag="VHV99_29075"
     CDS             4298..4828
                     /locus_tag="VHV99_29075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016348090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4381 domain-containing protein"
                     /protein_id="HEX3639463.1"
                     /translation="MSDASLVPPDSSGQLKALQELPLPETVSYAPHTIGWVFVAVLLL
                     VALGAVFWLARRRRAKDCYRRAALAELASIEASLASAHANREQRADALAAIPRLIKRT
                     ALVAAPREQVAALSGGEWLAFLQRTRGRFDARSGALLALASYAPPAAVAGISDGDAAA
                     LIGHTRDWIERHHVEI"
     gene            4815..5852
                     /locus_tag="VHV99_29080"
     CDS             4815..5852
                     /locus_tag="VHV99_29080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX3639464.1"
                     /translation="MWKFDFPWMFALLPLPLFVWWLVPAYRTRACAVRVPFFHEMAEA
                     AGEKPAPGGVRLRRNWLQRLLLPLLWTLLVIAAAKPVYVEAPIVREEPARDLMLAIDL
                     SGSMATRDFVDAAGVRMDRLSAVKSVVADFIAKRKGDRLGLVVFGDAAYPQAPLTLDH
                     DAVQVLLDQMQIGMAGPRTAIGDAIGLTVKLMANSPAPEKVLILLTDGNDTSSAIPPE
                     RAAEIAKDHKLVIHTIGIGDPNTTGEDKVDLDALARISSITGGRAFRALGRQQDLADV
                     YATLDKLTPEKVKREIYRPQRDFYWVPAGLTVAIFALYHVLALLITVLRAPRRERPAE
                     RATIAEARRGN"
     gene            5842..7434
                     /locus_tag="VHV99_29085"
     CDS             5842..7434
                     /locus_tag="VHV99_29085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX3639465.1"
                     /translation="MEIDLTAFHFLRPGWLLLLIPAVLLPFVWFRRNSVRARWRNVID
                     PVLLEHLIVGDTKRHVIQPVHTLALLLGIGALAAAGPTWQQERPPFNQDKAPLVVVLE
                     LAHSMDATDVAPTRLERAKQKVLDLARARKGARTGLVVFASTGHLVVPPTEDPAMLEL
                     YVPALSPALMPRDGKNATAGLDAAERLLENDPAAGTIVFMSDGFDTNQTDAFVKKAKA
                     LRHQLLWLAVGTANGGPVRGPDGMIAMDPEGHPLMGTFDADAIRKVANDADIPLASMR
                     VDDDDVVWVQHRAQAYLAQEEETKIVPRWKETGYWLVVPLLLLALWSFRRGWTVKWLP
                     AVLISLAFSAMPRHALAAEWRWIDLFATHDQQGRWNFEHGDYRVAAQRFDDPMWKGRA
                     QYLAGDYAGALESFSRLKTAPAYFYMGNTLAHLDDYAAAIKAYDNALKLQPALREAAT
                     NRAFLQRLLAKDQQDEMDPEEEAPDQVETDKKKGRGTSTVIEGAPRPSEEVWMRSLNT
                     SPAVFLQQRFEAESEAKGAGAP"
     gene            7431..8780
                     /locus_tag="VHV99_29090"
     CDS             7431..8780
                     /locus_tag="VHV99_29090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639466.1"
                     /translation="MTATFLRFLLVIAWLGATGTAPLAFADPAPRTMVRAHLEPAGPV
                     VAGSEVKLVVDLLTTTWFTEAPNWPLFTVADAIVSLPDEQADNLSEDIDGVRWFGVSR
                     AYRIAPQSGKTYDIPPFAITVYPGGTNAPVQVMTPALKLVTTLPAGAQDMAVFFPTAK
                     LTATQKIEPSTQQLRVGDSIMRTITQSAAGTESMLIPPANLGEVDGLKRYAKPSTTRN
                     IVQDRVGLVAGERTDSVTYIADHNGRFKLPPVTIEWWNTKTQKKETIALPGATFSAST
                     AREKPLFEIPADVMGQGIPHRIIVIHSGQLIAGCAIVLGLLVVAWARGRIVMFLRRAK
                     HRLNDARERWLASDTVAWRTLCAVARKGEWRRTIPALYRWMDRSGEFGHPARLANLPR
                     KDEPKVAELADAVTAQFAEGATREFRWAETETALLRVWKRQQVQRQERAPLPPLNEY"
     gene            9056..9532
                     /locus_tag="VHV99_29095"
     CDS             9056..9532
                     /locus_tag="VHV99_29095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010109551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3639467.1"
                     /translation="MTIQKITPFLWYATEAEEVAAFYTAIFPDSRITRVTAVPSAGAT
                     KVVDFVLFGQPFIAMSSGGGDPFNHAMSLLVNCEDQAEVDRYWNALLEGGGSTDACGW
                     LKDRFGVSWQICPSELLRMMADPDPVKATRVAEAMMKMVKLDLATLKAAYAGTTTG"
     gene            complement(9606..10649)
                     /locus_tag="VHV99_29100"
     CDS             complement(9606..10649)
                     /locus_tag="VHV99_29100"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014619016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX3639468.1"
                     /translation="MAKIFIYGAGSIGCYVGGRLLAGGSDVSFIGRARMAEQLRRQGI
                     TLSRHDNSRWYVPPERTDVSTDAASAATADLVLVTVKSAATPTAAAELASVLRPGTIV
                     VSFQNGVGNADVLRAALPQHTVLEGMVPFNVVERGPGAFHQGSAGELEIRHTPAMQPF
                     VDAFRQAGLPLIQHVDMLPVQWAKLLLNLNNAINALANRPLKEELSQRAYRRCLAMAQ
                     KEALALLKRAGIRPARVTPLPSNWVPHVLAVPDAVFERLGRTMLTIDPLARSSMSDDL
                     AAGRATEIDWINGEVVRLAQRLGQTAPVNARLSELVRQAERADARPAWSGEALLAELQ
                     AAADAPLVLHGNI"
     gene            complement(10810..11577)
                     /locus_tag="VHV99_29105"
     CDS             complement(10810..11577)
                     /locus_tag="VHV99_29105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX3639469.1"
                     /translation="MASSTVSLKNDVSSAEWEARVNLAAAYRLTALFGWDDLVFTHIS
                     ARVPGPDHHFLINPYGMMFDEITASSLVKIGLDGRKVAESPYDVNPAGFTIHSAVHAA
                     REDAHCVMHTHSVNGVAVSAQEAGLLPLSQQSLGVLASLGYHDYEGIALNEAEKPRLV
                     QDLGSNTYLMLRNHGLLTIGATPADAFVAMYFFEAACMIQVRAQAGGGKLRPIPQPIL
                     NGIKEQIGLVTRGVTPGALAWPGLLRRLNRRNPGYAD"
     gene            11772..>12292
                     /locus_tag="VHV99_29110"
     CDS             11772..>12292
                     /locus_tag="VHV99_29110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007737480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX3639470.1"
                     /translation="MESSLHRYRSQLETTPWFRGLPVELRDYLVSRATLLTLEKGQIL
                     YRRGDQSYGLYAVLGGALAIGTVGMDWKEALLAVLGPTAWVGEISMFDGLPRPNDATA
                     VSRTLLLHVPESALKSLLDSTPRYWRDFALLMAQRLRVSFENAEAMTLLPAAQRVANR
                     LLMIAGGYGGLNAS"
BASE COUNT         2264 a         3823 c         3955 g         2250 t
ORIGIN      
        1 gctggctcgc caacgcggcg tagtcgttgg caacgagtgc cttgaaagcc ggattgttgt
       61 tccactcggg atgcttcggc gccgccgctt tcacctggtc gagcatgaag gcgaactgga
      121 agtacagcgg ctgctcgctc cacagcgtgc cgtcgttgtc gaacacggcg atgcgcccgg
      181 ccggcgggtc gaagttcggc gagcctgcac gggtgacgtc ggcgacgaat ttctcgatcg
      241 tttgtttggc cgcgccgtca cgccaggacg gcagcgtggc gctcgaactg actgtcgcgg
      301 gcgatgatgg tgcgttcgta acgccaggcg tcgtgcaacc ggcggcgcct agtgccacga
      361 cgagcgcgac gagagcagcg ccgaacgccg acccgaatgt cgagccgaat gtcgagccga
      421 atgttgaccg ggatgcggac ccggatgcgg acccggatgc agacccggat gcagacccgg
      481 atacagaccc ggatacagac ccggatacag acccggatgc agacccggat gcagaccggg
      541 ataccggccc gaatacaggc acacccgcag cgcggatcga ccccgcagcc tgctccgacc
      601 atcctggctc acagctcgaa cgcatccagc ctccattccg attgcacaat tccatcaccc
      661 gaggcgacac gcgcgcggga gaaacggcac cagctcgaaa gggcggagcg ccctagcttc
      721 cgtctgcgtc ggcggcgcgg gccgatgcct gacgtgcaag cggctgcttc caggtgtctt
      781 cgtccatcac gagacgaaag ccgagatgcg acatgctgtt gtaggggtcg gtgccgcgcc
      841 gcgcgcttgg acgatagctc aggcagtaat cctcattgca gaggaacgag ccgccgcgtg
      901 tgacgcgctt gggggcgtcc acagggacac cctggtcggc cggatcccac gagtcgcgtg
      961 gaccggccgg attgtcgatc gtgctggtgc tcaccgcttc acgccggaac tggtcggcgc
     1021 gataccagtc ggctacccac tgccaggcgt tgccggtcat gtccgacagc ccgtagccgt
     1081 tcgccggaaa agtgccgacc ggactcgtgc cgagtgcccc gcccgccttc gggctgacca
     1141 ccgggaatga ctgcacttgc tggccctgcc agacgttggc catctgcttg ccgttcggcg
     1201 cgaactgatt gccccaggcg taagtggcct gttcgagccc gccacgcgcg gcgaactccc
     1261 attcggcttc ggtaggcaag cgcttgccgg cccatttcgc ataagcctgc gcatcttcgt
     1321 aggaaacctg aacgaccgga tgatcgtcct tgccgatgat gttgctgtcc ggtcccgtgg
     1381 gatgacgcca gttcgcgccg ggcacatagc gccaccattg cgaatagtct tccagcggca
     1441 ccggacggtt cgtgccgacg aacaccatcg cccccgcgac cattgcgcta tcgggcgggc
     1501 gcggcgtgcc gggcggcaat tgcaccttca aggtgtccca atcaggtttc ttctcggcgg
     1561 tggtgacgta gccggttgct tcgacgaatt tgcggaattc tgcgttggtg acgtgatgac
     1621 ggtccatcca gaaaccgtgc acacgcacct tgtgggcggg gcgctcgttg ggctgcgcct
     1681 gcttcgagtc gctgcccatc aggaattgtc cgccgggaat ccatgccatg cccagcggac
     1741 catgcctccc gtcgccatag acaacttgcg gatgaggttg attatgcgag gtgacagccc
     1801 aactgaccgc cgcgccgacg catgcgggaa gcatgacgag aagccctccc cacaggagca
     1861 gcttgcgata gcgcgcggcg ggcgtgcctt tagcgcgagc atccgttacc gtgctacgtt
     1921 ttcctgaact catcatccct tctctcacac gggtgaagcg gcagaccctt tagcaagcac
     1981 gggccggctc ccttttgcac ggaagaagct tagcgatacg gaataacgat ttatgccgaa
     2041 tagaaatgtg tcgcttgctg gttagcaaag ctgacacgca ggctatcaat tttttaatca
     2101 catttcacgc tttttttact tatgagcgaa actttgacga aagaccgctc gtgcattaac
     2161 gtatctggcg gcgatcgtcg gcattcaagt tgtcccacca gcaaaagaat cgggaatcga
     2221 gatctatgcg cgaaacgatc ctgcgtttcg aatcgagtgt caacgaagcg atagtgggcc
     2281 agcaagccgt ggttcggcag atactgattg cactgctggc agacggtcac gtgctgctcg
     2341 aaagtctgcc cggactcgcc aaaacccgta ccgtcaaagc cattgccacg cgcctcgcgg
     2401 ccaccatgag ccgcattcag ttcacccccg acctgttgcc gtccgacatc accggcgctg
     2461 aaacgttgct gcaaagcggc gcggaacgca cgcttagttt tcagcccggt ccgatcttcg
     2521 gtaatctgat tctcgccgat gaaattaatc gtgcgcccgc caaagttcag tccgcgttat
     2581 tggaagcaat ggaagagcgg cagatttccg tggcgggtaa aacgcataaa atgtcggatc
     2641 tgtttcttgt catggccacg caaaatccga tcgaacagga aggcacttat ccactgcctg
     2701 aagcgcaaat ggaccggttt cttcttaagg tgtcaatgag ttatccgtcg ccacaaagcg
     2761 aggtcgatat gttgcggctg ctgcggcgcg aaagcggcga ggcggacaac atgcccaatg
     2821 tgctcgcaca ggaggtcatt ttcgcggtgc gtgaagaaat caggcgcatc acggttgctc
     2881 cggcggtgga cgactacatt gtgtcgctcg tcaatgccac ccggcatggt agggaactcg
     2941 acgccgagct cggcaaatgg atcgaaatcg gtgcgagccc gcgcggttcg atcggccttg
     3001 accgcgcgag ccgcgtgcag gcctggttgc aggaccgcac attcgtcacg cccgacgacg
     3061 tgcgcgcggt cgttcatccg gtactgcggc atcggctgat actgtcttat gacgccaatg
     3121 ctgaaaatat cagtgccgac gccgtggtcg accggctgat cgaaaaggtt gccgtgccgg
     3181 cctgaggcac ggcgcggagc acggagcacg gagcgcagag cacaaagcac aaagcacgga
     3241 gcgcagagca ctgagtgcgc agcgcacagc gtatagcgca tggcgcgaca cgcatcgtcg
     3301 catcgacgca ggggatcgcc gtggtcttga tgtccgaatc cgcaagctgc gcaccgtcag
     3361 ccgatggccg cgtgtatgtc gacgcagtcc atctcgcgaa gctcgagttt catgcccgcg
     3421 ggctgagctt tatcgcgccg gcgccggtat cgagtgtgct gtcgggcagt cacgcgtcgc
     3481 gcttgcgcgg gcgtgggctg aatttcgagg aaattcgcgc ctacctgccc ggcgacgatg
     3541 tccgtcacat cgactggaaa gtctcgctgc ggctcggcaa gccgcagatt cgcgcctata
     3601 ccgaagagcg tgaccggccg ctgctggtgg tggtcgacca gcgcatgagt atgttttttg
     3661 gctcgcgccg cgcgttcaaa tcggtggtgg ccgcggaagt ggccgcgctt gccgtgtgga
     3721 tgggcttcac ggccggcgac cgggttggcg gcgtgctgtt cgacgacagc cgcatcgtgc
     3781 gggtgaggcc gctgcgcagc cgcagccgga tcaaggttct gttcggcgcg ctcgccgcga
     3841 tgaaccgcgc gctgcatgcc gatagcgaag cgcgcaccga ctacgcgcaa ctgaacgcgg
     3901 cgctcgaagg cgtgcttcag ctagccggtc acgattacct gatctgtatc gtcagcgatt
     3961 ttgccggcgc ggacgaacgc acgcagcggc tgttgcggca gttgtcggcg cacaacgacg
     4021 tcgtcgcggc aatggtcttc gatcccttat ggcagcacat gcccgaacac cgcgcgctgg
     4081 tggtcagcga aggacatctg caggtggaac tgcggatcga gcaggagcgc gtccgtgcac
     4141 cgttgtcgag cctgttcagc ggccgcaccg ccgaggtggc ggagttgctg cgttcgagcg
     4201 gcgtaccgct gatagccctc tcgacagcgg agccgacggt cgagcaggtg cggcggttgt
     4261 tcggccagcg cgcgcgaccg gccacgggga gcggtctatg agcgatgcgt cgctcgtccc
     4321 accggattct tcgggacaac tcaaggcgtt gcaggagttg ccgttgccgg aaacggtgtc
     4381 gtacgcgccg catacgatcg gctgggtgtt cgttgccgtt ttgctgctag tggcgctcgg
     4441 cgcggtgttc tggcttgccc ggcgccgccg cgccaaggat tgctacagac gggccgcgct
     4501 cgctgagctg gcaagcattg aagccagcct tgcgtccgcc cacgcgaacc gtgaacagcg
     4561 tgcggacgcg ctcgccgcca taccgcgcct gatcaagcgc accgcgctcg tggccgcgcc
     4621 gcgcgagcag gttgcggcgt taagcggcgg tgaatggctc gcgtttttgc agcgcacacg
     4681 cgggcgcttc gatgccagaa gcggcgcgct cctcgcactc gcgagctatg cgccgcccgc
     4741 ggcggttgcc ggaatttccg acggcgacgc ggcagcgctc atcggccaca cccgcgactg
     4801 gatcgagcgt catcatgtgg aaatttgatt ttccgtggat gttcgcgctg ctgccgctgc
     4861 cgcttttcgt gtggtggctg gtgccggcct atcgcacccg ggcctgcgcg gtacgcgtgc
     4921 cgttctttca tgaaatggcc gaggccgccg gcgaaaagcc ggcgcccggc ggcgtgcgct
     4981 tgcgccgcaa ctggctgcaa cgtctgctgc tgccgttgct gtggacgttg ctcgtgatcg
     5041 cagccgccaa accggtctat gtcgaagcgc cgatcgtgcg cgaagaaccc gcgcgcgatc
     5101 tgatgctcgc catcgacctg tcgggttcga tggcgacgcg cgatttcgtc gatgccgccg
     5161 gcgtccggat ggaccgcttg agcgcggtca aaagtgtggt ggccgatttc atcgcgaagc
     5221 gcaagggcga tcgcctcggc ctggtggtat tcggcgacgc cgcctatccg caggcgccgc
     5281 tcacgctcga tcacgacgcc gtgcaggttc tgctcgacca gatgcagatc ggcatggcag
     5341 ggccgcgtac cgccattggc gacgcgatcg gcctgacggt gaagctgatg gcgaattcac
     5401 cggcgccgga aaaggtgctg atcctgctga ccgacggcaa cgacaccagc agcgccattc
     5461 cgcccgagcg cgccgccgaa atcgccaaag accacaaact ggtgatccac accatcggga
     5521 ttggcgatcc gaacacgacc ggcgaggaca aggtcgatct cgatgcgctc gcccgaattt
     5581 cgagcattac cggcggccgg gcgtttcgcg cactcggccg gcagcaggac ctcgccgacg
     5641 tctacgccac gctcgacaaa ctcacgcccg agaaagtgaa gcgcgaaatc taccggccgc
     5701 agcgcgattt ctactgggtg ccggccgggc tgacggtggc gattttcgcg ctctaccacg
     5761 tgctggcgct gctgatcacg gtgctgcgcg cgccacgtcg cgagcggcct gccgaacgcg
     5821 cgaccattgc ggaggcccgt cgtggaaatt gatctgacgg cttttcattt tctgcggccg
     5881 ggatggttgc tgctgctgat tcccgccgtg ctgttgccgt tcgtctggtt tcgtcgcaac
     5941 agtgtgcgcg cccgctggcg caacgtcatc gatccggtgc tgctggaaca cctgatcgtc
     6001 ggcgacacga aacgccacgt gattcagccg gttcacacgc tggcgctgct gctcggcatc
     6061 ggtgcgctcg ccgccgcggg gccgacctgg cagcaggagc gcccgccatt caatcaggac
     6121 aaggcgccgc tcgtcgtcgt tcttgaactg gcgcattcga tggacgcgac cgacgtcgcg
     6181 cctacccggc tcgagcgtgc caaacagaag gtgctcgatc tcgcgagggc gcgcaaaggc
     6241 gcgcgcaccg ggctggtggt gttcgcctcg acgggccacc tcgtcgtgcc gcccaccgag
     6301 gacccggcca tgctcgaact gtatgtgccg gcgctgtcgc ccgcgctgat gccgcgtgac
     6361 ggcaagaacg ccaccgccgg actcgatgcc gccgagcggc ttctggaaaa cgatcccgcc
     6421 gcgggcacga ttgttttcat gagcgacggc ttcgatacga atcagaccga cgccttcgtg
     6481 aaaaaggcca aggcactgcg gcatcaactg ctgtggctcg cggtcggcac ggcaaatggc
     6541 gggccggtac gcggccccga cggcatgatc gcgatggacc ccgaaggcca cccgctgatg
     6601 ggcactttcg atgccgacgc gatccgcaag gtggcgaatg acgccgacat tccgctcgcc
     6661 agcatgcggg tggatgacga cgacgtggtg tgggtccagc atcgtgcgca ggcctacctc
     6721 gcgcaagagg aagagacgaa gatcgtgccg cgctggaaag agaccggcta ctggctcgtc
     6781 gttccattgc tgctgctcgc gttgtggagt ttccggcgcg gctggaccgt caagtggctg
     6841 cctgcggtgc tgatttcgct cgccttcagt gccatgcccc gtcatgcact ggctgccgaa
     6901 tggcgctgga tcgatctgtt cgccacgcac gatcagcaag gccgctggaa tttcgagcac
     6961 ggtgattaca gggtagccgc gcaacgcttc gacgatccga tgtggaaagg gcgcgcgcaa
     7021 tatctcgcgg gcgactatgc cggcgccctc gaaagttttt cgcgtctgaa gacggcgccg
     7081 gcgtatttct acatgggcaa tacgctcgcg catctggatg attacgccgc cgccatcaaa
     7141 gcctacgaca acgcgctcaa acttcagccc gcgctgcgcg aggcggcgac caatcgtgcg
     7201 ttcttgcagc gcttgctcgc aaaagaccag caggatgaaa tggacccgga ggaggaggca
     7261 ccggatcagg tcgagaccga caaaaagaaa gggcggggca cgtcgaccgt gatcgagggg
     7321 gcgccgcgtc cgagcgaaga ggtctggatg cgcagtctga atacctcgcc cgcggttttt
     7381 ctgcagcagc gctttgaagc ggaatccgag gccaagggag cgggcgcgcc atgacagcga
     7441 ctttcctgcg ctttctgctc gtcatcgcat ggcttggcgc gactggcact gcaccgctgg
     7501 cctttgccga tcctgcaccg aggaccatgg ttcgcgcgca tctcgaaccg gccggccctg
     7561 ttgttgcggg cagcgaggtg aagctcgtcg tcgatctgct gacgaccacc tggtttaccg
     7621 aggcgccgaa ctggccgctc ttcaccgtcg ccgatgcgat cgtgagtttg ccggacgagc
     7681 aggccgacaa cctttccgag gacatcgacg gcgtgcgctg gttcggcgtg agccgcgcct
     7741 atcgcattgc gcctcagtcc ggcaagacct acgacatccc gccgtttgcg atcaccgtct
     7801 acccgggtgg cacgaacgcc ccagtgcagg tgatgacgcc cgcgctcaaa ctcgtgacga
     7861 ccttgccggc gggcgcgcag gacatggccg tattctttcc gaccgcgaag ctcacggcga
     7921 cgcaaaaaat cgaaccgtcg acgcagcagc tacgcgtggg cgattcgatc atgcgcacca
     7981 tcacgcaaag cgcggccggc accgagtcga tgctgattcc gccggccaat ctcggcgagg
     8041 tcgacggatt gaagcgctac gccaaaccgt ccacgacccg caacatcgtg caggaccggg
     8101 tgggactggt cgcgggcgag cgaaccgaca gcgtgaccta catcgccgat cacaacggcc
     8161 ggttcaagct gccgcctgtg acgattgaat ggtggaacac gaagacgcaa aagaaagaga
     8221 cgatcgcgtt gccgggggcg acgttttccg cgtccaccgc gcgcgagaaa cccctcttcg
     8281 agatccccgc cgatgtgatg ggccagggca ttccccaccg aatcattgtg attcatagtg
     8341 ggcagttgat cgccggctgt gcgatcgtgc tgggcctcct ggtggtggcg tgggcacggg
     8401 ggcgcatcgt catgttcttg cgccgtgcaa aacatcggct aaacgacgcg cgcgagcggt
     8461 ggctcgcaag cgacacggta gcgtggcgca cgctatgcgc ggttgcgcgc aagggcgaat
     8521 ggcggcgaac cattccggct ttgtatcgct ggatggatcg gagcggagag ttcggacacc
     8581 cggcgcggct cgccaatctc cctcgcaagg acgagccgaa agtagcggag ctggccgatg
     8641 ctgtaaccgc acagtttgcc gaaggcgcca cgcgcgaatt tcgctgggcg gagacggaaa
     8701 ccgcgctgtt acgcgtttgg aaaaggcaac aggtgcagcg gcaggaaagg gcgccgcttc
     8761 cgccgctcaa cgagtattag gaaatagaga tccctcttga gcgagtgcag gaaacgcgca
     8821 tgaggcgcgt tgggaatatg tcagacgccg ggtggaagag atcgagttag tcggcgcgga
     8881 ggctcggcgc agtcatgaac agcagggcaa ctggggtcat acccggatgg ccgaagcgga
     8941 taaaaaagtg aaacttttct tcctggcctg tcgatcttga tgctattgct tcgtcgtccc
     9001 aatggcggca tgcggcttgc ctgcacatat agccatcact gaaaggagat ttgccatgac
     9061 gattcaaaag atcacgcctt ttctttggta cgccacggaa gctgaagagg tcgcagcgtt
     9121 ctatacggct atctttcccg actcgcgcat cacgcgggtc acggcggtgc ccagcgccgg
     9181 tgccacgaag gttgtcgatt tcgttctgtt tgggcaaccc ttcatcgcca tgagctccgg
     9241 gggaggtgat ccgttcaacc atgcaatgtc cctgctggtc aactgtgagg atcaggcaga
     9301 agtcgaccgc tattggaacg ccctgctcga gggcggcggt tcaacggatg cgtgcggctg
     9361 gctgaaagac cgcttcggcg tctcgtggca gatctgtccg tccgagctac tccggatgat
     9421 ggccgaccct gatccggtga aggccacgcg tgtggccgag gccatgatga aaatggtgaa
     9481 gctggacctc gccacgctca aagccgccta tgcggggacg acgaccggtt aaccaggacc
     9541 caggccacgt agcgttcgag acgcttcgag gatggatatt gaagaacggc tgtgcatgcc
     9601 aatgttcata tattgccgtg caataccaaa ggcgcatccg ccgcggcctg caactcggcc
     9661 agcagtgctt cgcctgacca tgcggggcgc gcgtcggcgc gttccgcttg ccgcacgagt
     9721 tcggagagcc gcgcgttgac gggtgccgtt tgaccaaggc gttgggcgag gcgcacgacc
     9781 tcgccgttga tccagtcgat ttccgtggcg cgccctgccg ccaggtcgtc ggacatcgac
     9841 gagcgcgcga gcggatcgat cgtcagcatc gtgcggccca gccgttcgaa cacggcatcg
     9901 ggtacggcga gaacatgcgg tacccagttc gacggaagcg gcgtcaccct ggccggccgt
     9961 atgccggccc gtttcagcag cgcgagcgct tctttctgcg ccatggcgag gcagcgccgg
    10021 tacgcgcgtt gcgagagttc ctccttcaaa ggacggttcg ccagcgcgtt gatcgcattg
    10081 ttgaggttga gcaacagctt cgcccactgc accggcaaca tatcgacgtg ctggatcagc
    10141 ggcagaccgg cttgcctgaa tgcgtcgacg aacggctgca tggccggcgt atgtctgatt
    10201 tccagctcgc ccgctgaacc ttgatgaaac gcgccagggc cgcgctcgac gacattgaag
    10261 ggcaccatcc cctccagcac cgtgtgctgt ggcagcgcgg cacgtaagac gtccgcgttg
    10321 cccacgccat tctggaagct cacgacaatc gtgccgggcc gaagcacgct ggccaattcc
    10381 gcggcggccg tgggcgtcgc ggcagacttg acggtgacca gcacgaggtc ggcggtggcg
    10441 gcgctcgccg cgtccgtcga cacgtcggtc cgctccggcg gcacatacca gcgactattg
    10501 tcgtgccgcg acagcgtgat gccttgacgg cgcaactgct cggccatgcg tgcacgcccg
    10561 atgaaactga cgtcgctgcc tccggcaaga agccgtccgc cgacatagca accaatcgaa
    10621 ccggcgccgt agataaagat ttttgccatt taggtcctca cgtgacttac ccacccatcc
    10681 gcgatgcatt ggatcggcaa acgcggttca tgacgcttcc gccgttcgct cagcatctat
    10741 catcgtgcgt agcacgtagc acgtagcacg tagcacgtag cacgcagcgc atgttgcgaa
    10801 cacaccgcgc tagtcggcat atccggggtt acggcgattg agccgacgca gcaacccggg
    10861 ccacgccagc gcccccggcg tgacgcccct cgtcaccagc ccgatctgct ccttgatacc
    10921 gttgagaatc ggctgcggga tgggtctcag ctttccgcca ccagcctggg cacgcacctg
    10981 aatcatgcag gccgcttcga agaagtacat ggcgacgaag gcgtccgccg gcgtcgcgcc
    11041 gatcgtcagc aagccgtggt tgcgcagcat cagataagtg ttgctgccga ggtcctgaac
    11101 gagacgaggc ttctcggctt cattgagtgc gatgccctcg tagtcgtggt agccgagcga
    11161 cgccagcaca cccagcgact gctgggaaag cggcagcaag cctgcctcct gcgccgacac
    11221 ggcaacaccg ttgaccgagt gagtgtgcat cacgcaatgc gcgtcttcgc gtgccgcatg
    11281 cacggcgcta tggatcgtga agccggccgg attgacgtca tacggcgatt cggccacctt
    11341 gcggccgtcg aggccgatct tgaccagcga agacgcggtg atttcgtcga acatcatgcc
    11401 gtacgggttg atcaggaaat ggtggtcggg gccgggcacg cgtgccgaga tgtgggtaaa
    11461 taccagatcg tcccacccaa acagcgccgt gagacggtac gcggccgcga gattgactcg
    11521 cgcttcccat tcggcagatg acacgtcgtt cttcagactg acagtagatg aggccatgtc
    11581 gacactcctt attcgatgat tcaagtaatg gaacaacttg gtggaacaac gcccggcgaa
    11641 atcgcccatt gggtccctgc gatgccgcag gtgcatcaag ggcgaaagtc attgcgtcgt
    11701 ccgacgcgag tccacatgat gtgtgcgaac ccgcctaaaa tctgtcagat acacgacagg
    11761 tcgacgcgcc aatggaatcc agcctgcacc gctaccggtc gcaactcgaa acgacgccgt
    11821 ggttccgggg cctgcctgtc gagttgcggg actacctcgt cagccgtgcc accctgctca
    11881 cgctggagaa gggccaaatc ctgtatcggc gcggcgacca gtcttatgga ctgtacgccg
    11941 tgctgggcgg cgcgctggct atcggcacag tcgggatgga ttggaaagag gcgttgctcg
    12001 cggtgctcgg accgacggca tgggtggggg aaatctcgat gttcgacggg ctgccgcgtc
    12061 cgaacgacgc cactgccgtc agccggacgc ttttgctgca cgtgcccgaa tccgcgttaa
    12121 agagcctgct cgattccacg ccccgttatt ggcgcgattt tgcgttgttg atggcgcaaa
    12181 gactgcgggt ttcgttcgag aacgccgaag cgatgaccct gttgcccgcg gcacagcgcg
    12241 tggcgaaccg tttgctgatg atcgccggcg ggtatggcgg tctgaacgcg tc
//
LOCUS       DATASH010001101         3994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2169130,
            whole genome shotgun sequence.
ACCESSION   DATASH010001101 DATASH010000000
VERSION     DATASH010001101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2169130"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VHV99_29115"
     CDS             <1..438
                     /locus_tag="VHV99_29115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3639471.1"
                     /translation="FGLGLTVGSTLGGKLADWRLMPSLVAFLLAIVVILTIFAGTMHA
                     EIPAMITIFVWGILAFAIVPPLQMLIVDRASHAPNLASTLNQGAFNLGNATGAWLGGM
                     AIGAGAPLTMLPWVGVATSIGALLLTLWSVSIDRRAQRMAVAG"
     gene            476..1642
                     /locus_tag="VHV99_29120"
     CDS             476..1642
                     /locus_tag="VHV99_29120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3639472.1"
                     /translation="MKVIFTRDFLALILSVAVVGLGSGATLPLTALALTQAGYGTDVV
                     GLLTATQAGGGLIVVPIAGWIAARFGGRQVIVSAVLVVALATASMQLTSNLWLWAMLR
                     IVCGAALMLLFTIGEAWVNQLADDATRGRVVAIYATNFTLFQMSGPVLVSQIADYTHW
                     RFLICGAIFLLALPALANIRKTPQASEDEHAAHGSWRHVLPQMPALVIGTGFFALFDT
                     IALSLLPLFAMAHGISSELAVLFASALLLGDTTMQFPIGWLADRLGRERVHVGCGVIV
                     ALLLPLLPWAITSPWLCWPLLLVLGAAAGAIYTLSLVACGERFRGVALVSASSLVGAS
                     WSAASFGGPLVAGALMKAVGNDAMVAVLMAAAFAFLAAVWWEKRRAPMQTDMSQ"
     gene            complement(1957..2313)
                     /locus_tag="VHV99_29125"
     CDS             complement(1957..2313)
                     /locus_tag="VHV99_29125"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HEX3639473.1"
                     /translation="MGDFGISCYDVADPIVSGNTIIGQNSPRNGIDLRSNRSAAVLIG
                     NVVEGFNGPDACGIAAEGRVGGVISGNFLRDCKTDIDLYQCKSMSVTGNSSTGCSRHY
                     AVESSERMIVENNANC"
     gene            complement(2317..3756)
                     /locus_tag="VHV99_29130"
     CDS             complement(2317..3756)
                     /locus_tag="VHV99_29130"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HEX3639474.1"
                     /translation="MSEPRRNFLARLATGAGAVIGGCAVAAGATLTAQRDDKHQAVSL
                     LDCMSSRVCKDALSGKPTLDHTREFQAALDSAASALIVPENCVFNLTGPLSVNKKIRL
                     SGGGELRFAGGIANAAAITVHASGCDFDGVFLTNPNLLQARTGNRNVGILFRANIGSV
                     TRSTVFQFQNGIQVEADGEYHDFIIANNKILDCIGAGGGPADTQSGYGEDRGDGITIW
                     GCCATVTGNLVTAKAGQDCRIGIHVEALAEYHAYSFPLQDNLCTIVGNVVRGAFRRGV
                     VTENVNNTAITGNTCEGSTWWGIAVVRSRSCTVTGNAVIVSRPASDRTGSSYGPIRAG
                     IQVYGGAIQCIISGNTVDVTQGSADAAISLQGLNSDSVAVLPKQRATANGTPNITLAI
                     ANPAIQIGQRISGTGVPPLTHVLSVAGAAVVASQSIKAASLEVTFTWHERGQGITVTD
                     NSVSCLAAGHVIGIWAQSQDNPTISNNSL"
     gene            complement(3769..>3994)
                     /locus_tag="VHV99_29135"
     CDS             complement(3769..>3994)
                     /locus_tag="VHV99_29135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639475.1"
                     /translation="NRYRQVIALFPAVRFDRLCFCFLFCFIATQALFEPDYGSYLRHL
                     SPIAPIILYLILRLRGLKEQAASQGSDQNS"
BASE COUNT          704 a         1218 c         1206 g          866 t
ORIGIN      
        1 ttcggcctcg gcttgacggt gggcagcacg ctcggcggca agctcgcgga ctggcggctg
       61 atgccctcgc tggttgcgtt tctgctggca atcgtcgtga tcctgaccat tttcgccggc
      121 acgatgcacg ctgaaatccc ggccatgatc acgatcttcg tgtggggcat tctggccttc
      181 gcgatcgtgc cgccgctgca aatgctcatc gtcgatcgcg cgagccatgc gccgaatctg
      241 gcatcgacct tgaatcaagg cgcgttcaac ctcggcaatg ccacgggtgc gtggctcggc
      301 ggcatggcga tcggtgcggg cgcgccgctt accatgctgc cgtgggtcgg cgtggcgact
      361 tcgatcggcg ctctgctgct gacgttgtgg tcggtgtcga tcgaccggcg cgcgcaacgg
      421 atggcggtgg cgggataagt gcgaatgacc cgcggccgta gtagcatctc ggccgatgaa
      481 agtcatcttc acccgcgatt tccttgcgct gatcctgagc gtcgccgtcg tcggactcgg
      541 cagcggcgcc acgcttcccc tcactgccct cgcgctgacg caagccggct atggcaccga
      601 tgtggtcggc ctgctgaccg ccacgcaagc aggcggcggc ctgatcgtcg tgccgatcgc
      661 cggctggatc gccgcgcgct ttggcggccg ccaggtgatt gtcagcgcgg tgctggtggt
      721 tgcgcttgcc accgcgtcga tgcaactcac ctcgaacctg tggctatggg cgatgttgcg
      781 cattgtctgc ggcgcggcgc tgatgctgct cttcacaatc ggcgaagcat gggtcaacca
      841 gctcgccgac gacgccacgc gcggccgcgt cgtcgcaatt tacgcaacca acttcacgct
      901 attccagatg tcgggcccgg tgctggtgag tcagatcgcg gactacacgc actggcgctt
      961 cctgatttgc ggcgcgatct tcctgttagc cttgccggcg ctcgcgaaca tccgcaagac
     1021 gccgcaagcg tcggaggatg aacacgcggc gcatggcagc tggcgtcatg tgctgccgca
     1081 gatgccggcg ctagtcatcg gcacgggctt cttcgcgttg ttcgacacga tcgcgctgtc
     1141 gctgctgccg ctcttcgcga tggcgcacgg tattagcagc gaactggccg tgctgttcgc
     1201 gtcggcccta ttgctcggtg acacgacaat gcaatttccc attggctggc tcgcggatcg
     1261 gctcgggcgt gaacgtgtgc atgttggctg cggcgtgatc gtcgcgctgc tgctgccgtt
     1321 gctgccttgg gcgatcactt cgccgtggct gtgctggcct ctactccttg tactcggtgc
     1381 cgcagccggc gcgatctaca ctttgtcgct agttgcttgc ggcgagcgct ttcgcggcgt
     1441 cgcgctggtg tccgccagct cgctggtggg cgcgtcgtgg agcgcggcga gtttcggcgg
     1501 cccgctggtg gcgggcgcgc tgatgaaagc tgtcggcaac gatgcgatgg tcgctgtgct
     1561 aatggcagcg gcgttcgcat ttcttgccgc cgtttggtgg gagaaacggc gtgcgccgat
     1621 gcaaaccgat atgtcgcaat aaatatttcc ccggtatatt ggggcctcgt gccatgcagc
     1681 atccgggcac cgcatcgcgt cgttttacgc gtgaaaaaaa tggctgttgc cgctaaggga
     1741 gtaggggatg gtcagttttt ttacgcatgc cgcatgtcta ttggtcggcg tggcgatcgg
     1801 cgtcttcgtg tgcgcgctat gcgttgcgcg atcaagaacg ccacgagacg acaagtgact
     1861 cggcgcttct attgatagct tccgcctggt tatagaagag cgcccgcatc gcgcgctgtc
     1921 gcgtcgaaaa cccttcgcga caacggctgt cttgcgttag caattcgcgt tgttctccac
     1981 gatcatgcgc tcgctgcttt ccacggcgta atgacgcgaa caacccgttg aactgttccc
     2041 cgtcacggac atggatttgc actggtatag atcgatgtcg gtcttgcagt ctcgcaagaa
     2101 gttgcccgag atgacgccgc caactcggcc ctccgccgcg atgccgcacg catccggtcc
     2161 attgaagcct tccacgacat tgccgatcaa tacggccgcg cttctgttcg aacgaagatc
     2221 gatgccgttg cgggggctgt tctgcccaat tatcgtattg ccgctgacga tcggatcggc
     2281 aacgtcgtag cagctaatcc caaagtctcc cattgctcaa agactgttat tgctgatggt
     2341 cgggttatcc tggctttgcg cccagatgcc gatcacatgt ccagcagcaa ggcaggacac
     2401 gctgttgtcc gtgaccgtga tgccctgccc ccgctcgtgc caggtgaaag tcacctcaag
     2461 cgatgccgcc ttgatagact ggctcgcgac cacggcggcg ccagcaacgc tcagcacatg
     2521 cgtaagcggt ggaacgcccg tgccgctgat acgctgcccg atctggatcg cgggattggc
     2581 tatggcgagc gtgatattcg gcgtgccgtt tgccgtggcc cgctgcttgg gcaagactgc
     2641 gaccgagtcc gaattcaatc cttgcagaga gatggccgcg tcggccgagc cttgggtcac
     2701 atccacggta ttaccggaaa tgatgcattg aattgcgccg ccatacacct ggattccagc
     2761 acgaatcggc ccgtaactgg aacctgtccg gtcacttgcc ggacggctga ctatcacggc
     2821 gttacccgtc accgtgcatg atcgcgagcg aaccactgcg atgccccacc acgtcgagcc
     2881 ttcacacgta ttgccggtga tcgcggtatt gttgacgttc tctgtcacca cgcctctgcg
     2941 gaacgcgcct cgcaccacgt tgccaacaat cgtgcacaag ttatcttgca gtggaaacga
     3001 gtacgcatgg tattccgcca gcgcttcgac atgaatgcct atgcggcagt cctgtcccgc
     3061 cttggccgtc acgaggttac cggttacggt ggcacagcag ccccagattg tgatgccgtc
     3121 tccccgatcc tcgccgtacc cggattgggt gtcggccggt ccgcctcctg cgccgatgca
     3181 atccaggatc ttgttgttgg caatgatgaa gtcatggtat tcaccgtccg cctccacctg
     3241 tatgccgttc tgaaactgga aaacagtcga gcgcgtcacg cttccaatat ttgcccggaa
     3301 caagataccc acatttctat tgcctgttcg agcctgaaga agattgggat tggtgagaaa
     3361 cacgccatcg aagtcacaac cgcttgcgtg cacagtgatt gccgcggcat tcgcaatgcc
     3421 gcccgcaaac cgcagttcgc caccacccga aaggcgaatt ttcttattta cactgagcgg
     3481 gccagtcaga ttgaaaacac aattctcggg gacaatcagc gcggacgcag cactgtcgag
     3541 tgcggcctga aactcgcgcg tgtgatccag cgtcggcttc ccggataatg catccttgca
     3601 cactctcgac gacatgcaat cgagtaggct gaccgcctgg tgtttatcgt cgcgttgcgc
     3661 cgtcaacgtt gcacccgcgg cgaccgcgca tcccccgatc acagcaccgg cccctgtcgc
     3721 aagccttgct aaaaagttgc gtcttggctc gctcattacg tagtgccatt aggagttctg
     3781 gtcggagcct tgcgacgctg cctgctcctt aagcccgcgg aggcggagta tgagatagag
     3841 gattatgggc gcaatcggtg acaggtgcct gagatatgac ccatagtccg gttcaaacaa
     3901 agcctgagta gcgatgaaac agaagaggaa acagaaacac agtctatcga agcgcaccgc
     3961 gggaaagagt gcgatcacct ggcgatagcg attg
//
LOCUS       DATASH010001102        26330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_7115557,
            whole genome shotgun sequence.
ACCESSION   DATASH010001102 DATASH010000000
VERSION     DATASH010001102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 26330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26330
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7115557"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="VHV99_29140"
     CDS             complement(<1..739)
                     /locus_tag="VHV99_29140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639476.1"
                     /translation="MGPTTALCSSWKVKVFEAAQALRTGGAGVSVMANAMAALHSIDA
                     HVPVEQAGQVIQHFFFKKQNGDLITSMPIHEIGEQLGHPNVNIQRRMLLRALAQQLTP
                     DTLTTGLRCVSYAHRPNGVRASFEDGSTHEADLLIGADGLNSVIRQQMLGETPTRPSG
                     YIAWLAVTPLSHPVMTEGYVAHYWGRGKRFGLCDVGDGQAYWWGTCNHGNAADAALSI
                     SKQEVLSACTGWAPEVIAAVEATPESGI"
     gene            complement(1200..2555)
                     /locus_tag="VHV99_29145"
     CDS             complement(1200..2555)
                     /locus_tag="VHV99_29145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3639477.1"
                     /translation="MDYSATPQPAGADALNLAGESGAQPAKRTSHAKAIAAITLGNGL
                     EFFDFTIYSFFATIIGKLYFPVEGQLAQLMLAVGTFGVGFIMRPVGGIVLGAYADRAG
                     RKAAMSLTLWLMTLGSAIIAFAPTYAAIGVAAPLLVILARLIQGFALGGEIGASTSLL
                     LEYGSNKTRGFYGSWQFVSQGLNTVCGSLLGVALAAALSTAALESWGWRVPFVIGMAM
                     GPIGIYIRRHLDETLPGIEDGKVPAAQVAAAQPVRTLFREHSRVITTGVLTTIGGTAA
                     NYIVLFYLSTYAIRILHLPMSSALWAAWTAAVVTVICSPFAGALSDRVGRKRVLWVSR
                     VLLIAAVYPAFMIINATPSVSVLLSVVAVLAVFVAFTAVPNIVMLPEMFPREIRATGM
                     SVVYCLGVSIFGGFAQFFATWLIQLSGSNLAPAWYLIGCGLVSLLPLPFMQETAGRAI
                     D"
     gene            complement(2746..3909)
                     /locus_tag="VHV99_29150"
     CDS             complement(2746..3909)
                     /locus_tag="VHV99_29150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HEX3639478.1"
                     /translation="MAIPAGIAELEDEMIALRRRIHAQPELAYEEFATGDLVAERLQE
                     WGYTVHRGLGQTGVVGQLKVGNGTRKLGLRADMDALPIHETTGLPYASTVAGKMHACG
                     HDGHTAMLLAAAKHLAREKSFDGTLNLIFQPAEEGQAGAKKMLEDGLFDRFPCDAVFA
                     MHNMPGFPTGKFGFLPGSFMASSDTVIIKVTGRGGHGAVPHKAVDPVVVCAQIVLALQ
                     AIVSRNIAPLDMAIITVGAIHAGEAPNVIPETAEMRLSVRALKPEVRDHLQERITAVA
                     CGQAAVFGARAHVDYQRRYPVLVNDAEMTGLARQVALDWLGEDGLIADMQPLTGSEDF
                     AFLLERCPGSYLIIGNGDGEGGCMVHNPGYDFNDDCLATGAAYWVRLAQTFLV"
     gene            4037..4987
                     /locus_tag="VHV99_29155"
     CDS             4037..4987
                     /locus_tag="VHV99_29155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX3639479.1"
                     /translation="MKLHQLSTLAAIADTGSIRAAARSLDLSPAAVTKAMRELEADLQ
                     APLLVRGASGVAFTEFGRALVVHARLVLGQLHRAQAEIEAMRGAAAGKLSIGVTPWVA
                     LTFLPPTVQRFRRRMPEVQLEFFEGLLAVVQPRLRDGSLDFSIGRPPPASPQSEFHNV
                     PLFSTHSAVVARRDHPKAGCRTLLELEDAEWVLNWDPASRESISDNLFRKRGMRVPHT
                     IHLAHSLAVVLGLLAQTDMLSIFPWPLVEVSIAKENLWALPLRETVDETIVSITSRRG
                     APTSPAAACFLDCLREVIDEGARSHDPELRRLFHSIELLL"
     gene            5225..6712
                     /locus_tag="VHV99_29160"
     CDS             5225..6712
                     /locus_tag="VHV99_29160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639480.1"
                     /translation="MKQTPANNEADYDTQLPGASAQRGSNSGSAGDNDTDSDSTSGSG
                     SASGSASGSGSGSGNDSTNASLDPSHHRALADARRTDGDELGAVAHLIAAHTLDAYAA
                     AAPDASAANLCDVATGYFMTGDHVIAAYWYRLVLTLDPRVAIACQNLAAILASAGDVA
                     EADAYRQRAYRIQRVFVEGSGAEQRRVLVLCAAHASGNVPFDALLPGTTCCRIKYAID
                     YAAAEEDQQLPAYDLVFNAIGDADIAAPLAARLARFVSHCARPVLNPPAAVARTQRHR
                     TVSLLDGLPDVQAAPCVQSDAAPASRAALDTLLAQSGIDFPVLARPAATHGGDGLTLC
                     DSLAALEAWRSAQTGVAYLTAFRDYRSADGFYRKYRIIFVDREPYPYHLAISPHWMVH
                     YYSAEMEQHPWKLEEERRFLDNPHAMLGERVMRAVAAIGRQLDLDYGGIDLTVLPGGQ
                     VLVFEANATMLTHFERKTGELAHKNPYVQRIVDAFERMMTRRTAT"
     gene            complement(6776..7234)
                     /locus_tag="VHV99_29165"
     CDS             complement(6776..7234)
                     /locus_tag="VHV99_29165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639481.1"
                     /translation="MKTTIAIALAALALSGCMSAEKQEAQARDFTRSENLLDAAQRNA
                     AIACVDQTQCDKAWALTKDYVAAHSSTSIVRADAVAIETDVPSRSGKAVYSATRVAKG
                     SGATITLYAQCRGMYGPERAQGSDYDDCVEQIGPTQNGFVPYLNQHLSSQ"
     gene            complement(7436..7957)
                     /locus_tag="VHV99_29170"
                     /pseudo
     CDS             complement(7436..7957)
                     /locus_tag="VHV99_29170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432545.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            complement(8152..9123)
                     /locus_tag="VHV99_29175"
     CDS             complement(8152..9123)
                     /locus_tag="VHV99_29175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3639482.1"
                     /translation="MLKAATFRQLKALHTVAKLGSVSRAAEELKLTQPAVSLQVRLLE
                     EAAGAPLLQRVGRGVQLTAAGEILARYALEILDLWNGAADDLAALHGEQGGTLRIGAI
                     TTAEYLIPPFLVRFTELRPQVKVQFKVGNRADIIRMLATHEIDLAVMGSAPRELRTVA
                     TAFAKHPMAFVASPAHALMKKKRLSLDDLQSANLFVRERGSGTRSTVENLFRLAGHKL
                     HIGSELSSNEAIKQLVEAGLGVAFLSLHACSLEFQAGLLALLPLPANPIERDWYVMHV
                     SDKRLPHVAGLFRDFLIEHGMSGAVPVPLPAVAAAKRRRQARQASPV"
     gene            9278..10669
                     /locus_tag="VHV99_29180"
     CDS             9278..10669
                     /locus_tag="VHV99_29180"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012365154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX3639483.1"
                     /translation="MAATDQQTPSGAQSTQPGLHRAPAVAHRVVIVGGGVGGLGLATR
                     LSETLGRAGHAQIVLVDRWPTHFWKPLLHEAASGQLDPATHQLQYAVQAQRHGFEFEQ
                     GELAALDRTSRHITLNALYDADGREILPSRQLAFDTLVLAMGSVTQYFGVPGAAEHAL
                     PLDSVAHAEAFRRKLLDACLRANHARRARTAQADTPVSINIIGAGATGVELAAALRDT
                     VRLLKHYSPFSLDPVRDFRIRLIESSERVLPALSETISARAQTMLGSLGVEVLNATRV
                     TEVRADAVLTSQGAPLASDIAIWTAGIAGPPVLRTLDRIAVNRNAQVQVNRTLQCTND
                     ANIFGLGDCAACPSSDQADAFLAPRAQVAHQQAMFLARALKCRIAGEPLPEFVYRDAG
                     TLVGFGREGTIGSLSNSLLTQPVFVDGWLATAVYKLIYRRHVMTLTGFARMALDSASH
                     WLRRRVHPVIRLH"
     gene            complement(10742..11053)
                     /locus_tag="VHV99_29185"
     CDS             complement(10742..11053)
                     /locus_tag="VHV99_29185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4148 domain-containing protein"
                     /protein_id="HEX3639484.1"
                     /translation="MAVDTIPLREPTPGPHEFYKLHEQVLQKPPVQGQRAPAVPAVQQ
                     VAQAGQALQAPRGKTRDEVRRELSAARAAGCMDVPDNQYPQPCPLPGAIRSMADAFRI
                     E"
     gene            12333..13016
                     /gene="irlR"
                     /locus_tag="VHV99_29190"
     CDS             12333..13016
                     /gene="irlR"
                     /locus_tag="VHV99_29190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010108925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal response regulator transcription
                     factor IrlR"
                     /protein_id="HEX3639485.1"
                     /translation="MRILIVEDEAKTGTYLRKGLTEAGFVADWVQDGVTGQHLALTED
                     YDLIILDVMLPGLDGWAVLKNLRHIRSTPVLLLTARDDLDDKVRGLEMGGDDYLIKPF
                     DFAELLARVRSILRRGHARDTASLHVADLALDLTRRKATRQGDTILLTAKEFALLWLL
                     MRRHGEILPRSTIASQVWDMNFDSDTNVVDAAIRRVRAKVDDNYEPKLIHTVRGMGYV
                     LEERDSAPR"
     gene            13085..14518
                     /locus_tag="VHV99_29195"
     CDS             13085..14518
                     /locus_tag="VHV99_29195"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012210999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HEX3639486.1"
                     /translation="MPRTLRVRLSILFAVSTSLLLVFNGVLLYHGLKTRLDSTSAREM
                     TATLSALQLSLHTMPGLDAVRADREIWYDHLHGHQNLDIALFDEGGAPLNRTSGYWPN
                     AESLAVRAGLAPVQVTVEGMPMRFLVAMAPLSGQADARVRVVVQYDASAEGALLSAYA
                     LNVSFVSVIGTLVNALLAWWIARFGLRPLARLTARAEQISSSRLARPLPERDMPGELK
                     ELSHAFNRMLARLHDSFTRLTQFSSDLAHDMRTPLTNLLAEAQVALSQPRTADDYRAV
                     IESSVDEFQRLSRLIDSMLFLARADSAQRRLALQTVDARSEALRVAGYYESMAADVGV
                     EIVVNGNANFEGDVLLVQRALSNLVSNALAHAPRGSTVELRCVERLHDVQLSVSDSGA
                     GIAEPHLERIFDRFYRVDPARHNSARGTGLGLAIVKSIMNEHHGECSVESMPHVRTTF
                     SLRFPKQPVALPTPKVPRGRFRRRHNK"
     gene            14630..14947
                     /locus_tag="VHV99_29200"
     CDS             14630..14947
                     /locus_tag="VHV99_29200"
                     /inference="COORDINATES: protein motif:HMM:NF025045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4148 domain-containing protein"
                     /protein_id="HEX3639487.1"
                     /translation="MKSRQIALAAILMSALSTSALASHDDSVRAPGDADRIAPPALTG
                     MPAAQPHQLGKTRAQVRKELIDARREGTIPTTEADYPQGQRTIAANRARNRIFENFWT
                     GKD"
     gene            14976..15602
                     /locus_tag="VHV99_29205"
     CDS             14976..15602
                     /locus_tag="VHV99_29205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003274526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEX3639488.1"
                     /translation="MTYPQRMLVENLAWSAEISNRDPAYFQRLASDQRPNVLWLGCSD
                     SRVPEAITRAMPGEIFVHRNIANLVSEHDDSLASVLEYAIVVLRVQHIIVCGHHCCGG
                     VQAALRPPAGALPNVNRRIDPIRRLAALHAAELRLLDASDERADRLAELNVLAQVQAL
                     HAMPLIQNAQRPVQIHGWIFSMHDGRLKALADPQWALSDADDVHEATG"
     gene            15752..17248
                     /locus_tag="VHV99_29210"
     CDS             15752..17248
                     /locus_tag="VHV99_29210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014252201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEX3639489.1"
                     /translation="MQAHATPPHDRSPRACPPAAWRTDLLAGISVFLIALPLCLGIAT
                     ASGVAPFAGILAGVIGGLVVALLSGSRLSVSGPAAGLVMIVVQALATLGSFSAFLTAL
                     VLAGALQLIFGFLRAGKLASYVPQPVINGMLAAIGILLIVNQLPLAAGFANGDISVAM
                     PLLALASLAILLAWETPALRAFALVRALPAPLAVVSFGIAATALVDTFAPGVALASSQ
                     RVAMPPLGSWDALRQAFTFPDLAQWLNADVWRVAVVIAIVASLESLLSLEALEQIDPE
                     HRKAAPDRELKAQGVGNLLAGLLGALPLTAVVVRSAANVHAGARGRLSCVTHGVVLLI
                     ACLALTRVINMIPLASLAAILIHTGYKLARPQLFVSMSQAGFAQFVPFIGTIVGVLAT
                     DLLIGIAIGFALSVVLVVERTIRRVLSFTRDGNHYLLTVRKSATFFCTPQLKHYLGQV
                     PSGATLILDATHADHIDHDVRQVVDSYAARARASGVRIEYKQWPPAHR"
     gene            complement(17285..18178)
                     /locus_tag="VHV99_29215"
     CDS             complement(17285..18178)
                     /locus_tag="VHV99_29215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006028391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="HEX3639490.1"
                     /translation="MLDYALLDALAAVVRHGSFDRAASELNVTPSAVSQRVKLLEERV
                     GSVLVKRGQPCVATTSGALLCRHTERVQLLEAELAGRLPALPGTLVEPWPALRVAVND
                     DSVGTWFIDAVARFCVEREMLLDLVVDDQDHTAQRIRDGSVQGAVTTQAEPVQGCRST
                     RLGRMRYLAVCTPEFRERHFADGVTRDTLRRAPCVDFNPKDQLQKRFMRRVTRAELDP
                     PLHWIPHVAGFLRACVMGLGWGMCPERMIAPHLASGELVDMAPGKHIDVDLYWQSWRL
                     SIGWLDDFGAMLRKRAAEFLD"
     gene            18276..18911
                     /locus_tag="VHV99_29220"
     CDS             18276..18911
                     /locus_tag="VHV99_29220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE/ArgO family amino acid transporter"
                     /protein_id="HEX3639491.1"
                     /translation="MNWLSFSHGAALCASLIVTIGAQNAFVLRQGIMRSHVGKIVALC
                     ALSDFILIGAGVGGASVLVERYPVFVHAMLYVGLAYLAWFGINALRRAVRPAHAVLDT
                     DASGAAPGTSTAPVQRAMPIVLMTLAFTWLNPHVYLDTFLLIGTAGAREPDGARVAFA
                     LGAMAVSGVWFIGLGYGARALAPLFRRASAWRVLDGAIGSMVLLLAVTQLR"
     gene            complement(18981..19250)
                     /locus_tag="VHV99_29225"
     CDS             complement(18981..19250)
                     /locus_tag="VHV99_29225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018432356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639492.1"
                     /translation="MTAAGPEAKMQASGWPVDDITGLGRRLPEVDPARYFDEQARESY
                     QLSLVKWPVLARLMKLVPLDEATVSAASTASALHDTVQAAAVDFD"
     gene            complement(19247..20797)
                     /gene="bcsG"
                     /locus_tag="VHV99_29230"
     CDS             complement(19247..20797)
                     /gene="bcsG"
                     /locus_tag="VHV99_29230"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004549777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HEX3639493.1"
                     /translation="MTLWNLYFIIKLYLFTGGHLQPVWIVNIGFALALAASATLRHRG
                     WRVLRNLVGLALGIPLMYYEANVPPFSRLIEEFGDLTSFSFGYWLELAQRFLPPMLLL
                     TALGALIGYLIVNRWLRVATFVLIALVAIPLWQQGSAVYARLQSGPATAASPNTLAAQ
                     PLDHNAALSAFRTQESQRQVTFGHLGADANTQFDVIVLHICSLSWDDLDAAKARNNPM
                     LSHFDYLFTNFSTAASYSGPAAIRVLRASCGQEAHADLYKDAPQQCHLLADLAQAGYT
                     PQTMLNHDGHFDNFLQLVHDNTGVPNVPMISNTSMPIAMHAFDGSAIRDDYATLANWY
                     AQRANTPGPVALYYNTISLHDGNRLPNSNLSSIDSYPQRVNKLMSDFDRFADLIAASG
                     RRAVIVFVPEHGAALRGDANQVAGLREIPTPRIVHGPAGVRLVGFQGNHGPTTVIDNP
                     SSFLAVAQLLSNLVSNSPFKPGVTLPQYAANLPQTQMVGENEGTVTMKTASGYVVKTP
                     DGVWVDGK"
     gene            complement(20840..23386)
                     /gene="bcsA"
                     /locus_tag="VHV99_29235"
     CDS             complement(20840..23386)
                     /gene="bcsA"
                     /locus_tag="VHV99_29235"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018432358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HEX3639494.1"
                     /translation="MSGPACVGRSERTRRQRAFDWLARGLGLPAERTALDWFVRLFFQ
                     PPAAGQADLARLGIRAAVLHLAAHWGVLEPHRLRAWLWRAFIRAPKPRAQTGAAKARA
                     VLAWIDRWLVPVLLRWRRVQRRIEAMLPSLPWERWGVQLEAGTQRIGHIRWLLPFIVV
                     AGAVVWSMIGTSPLQAEGQFEFFAVLAVVALVVRRFPGRMPVLILTTLALLAMVRYVW
                     WRVTQTLSFQTPGEAILGYLLFGAEAYTWLILLLGFVQTAWPLARTVAELPADTSSWP
                     TVDIYIPTYNEPLTVVRPTVFAAQGIDWPADRLRVYLLDDGTREEFKNFARDAGIGYL
                     TRSDNQHAKAGNINRALEKTHGDYIAIFDCDHVPTRSFLQTTMGTFLRDARCAMVQTP
                     HHFFSPDPFERNLGTFRRVPNEGNLFYGLVQSGNDLWNASFFCGSCAIIKRAALVEVG
                     GIAVETVTEDAHTALKLHRRGYTTAYLPTVQAAGLATESLAGHIKQRTRWARGMAQIF
                     RIDNPFLGRGLGLFQRLCYGNAMLHFFYGVPRLVFLTMPMAYLFFQMYFINASAITIA
                     GFVLPYIVLANIANSRMQGKFRHSFWAEVYESVLAWYIALPTTIAFFSPKHGKFNVTD
                     KGGRIAEGYFDWAVSKPYLVLLALNASALLAGVYRLTLGPGGETATILMNVFWTLYNL
                     VMLGAAASVAREAKQVRVTHRIAMRASATLLLADGTTVACITSDYSTGGLGLEVDPAL
                     KLALGDSLGVCLSRGDRQFHFPVHVSRFVGSHLGVRFDGLTLEQERQMVQCTFGRADA
                     WLNWDEETIEDTPLRGLKDVLTMGIEGYSRLLNGAARAVQSLLALDRTRY"
     gene            complement(23383..24183)
                     /gene="bcsQ"
                     /locus_tag="VHV99_29240"
     CDS             complement(23383..24183)
                     /gene="bcsQ"
                     /locus_tag="VHV99_29240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012213604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090540 - bacterial cellulose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HEX3639495.1"
                     /translation="MKTIAVVSTAGGTGRTTLSAALAVLLARRGRPVVALDFDPQNML
                     GAHLGLDCLAPAGLAQAMLDETAAWHAHTWRNSEGVLFVPYGAVSLEQSARCDARLAA
                     EPRWLANALAQLDLPREGVVLVDTARYPSQQGEQAVQSADLVLCVTPPEPAACATLVE
                     RLDALRRGGATLRIIVNRLNPARDMQRDALAMLGAALGDGLPLAQRVHLDAALPESFA
                     RGTWLFDEAPHSQASHDLQGLANWLHAWLAATLPDAPAANARRVDGRP"
     gene            complement(24180..24386)
                     /locus_tag="VHV99_29245"
     CDS             complement(24180..24386)
                     /locus_tag="VHV99_29245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006402171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639496.1"
                     /translation="MEFLLICFVAGLVLAIPVWIVWQRGGSRRGLAAIRHFDPRDAVP
                     CDIEPIALRARLLPEAERAPGGTS"
     gene            complement(24386..>26330)
                     /gene="bcsE"
                     /locus_tag="VHV99_29250"
     CDS             complement(24386..>26330)
                     /gene="bcsE"
                     /locus_tag="VHV99_29250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018432361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HEX3639497.1"
                     /translation="PGADALLWESAREAQARDVTVVLARARAQVAKRLRELGFSSHAP
                     ASGWPRNLSVLAMPPAGPGDAGPHGVVPVPLAKLFGGLRALKRFGFRSRSLYFVEGAE
                     RWFNWDDPAALAREGRMLANWCAVRDITVVLLLGSQSDVNADVNADADTDIDAAAGSL
                     FIEPTLDSAAAQLGRNEFHAACAGVARMERTHGELLWQVDFWRSDRTLVTGEVRSLRF
                     TDNGRLSVAPDPLSDAAARGDGLLARDEQRVIVSRAVVRGHEAWVPPNWEVLADQQAV
                     LAACVDAQAATIVLDYASGAQLEGLCAAIHALRRQAGRALKIVVAERGEVLRHQYELL
                     VLTLGANLVLGRDLPFSRMQSLLQSLQGQLHTRAVAADYRTALSAALSEDVLGYLPAG
                     TFCERVRAVLERSAVLQLPHVLVKLTLLPQCAHVEALKSCVPRRAGDIFTADASHLYV
                     FLFACRLADADAALARIFNVPVNRIADGIVHLAEQSIGDELKALATASRRAPIADYSD
                     LFATPLRSRTAAPGAAAHAGTVLPAEDGMSTVEQLAAVEQALDAAREEATQELAQKTG
                     QVASQQAGHKAEHEAGHEVEQKAGQKAGQKAGHEAGHEAGHKAGPAEASGPIAPAQPT
                     PRAVAPPRRAEPCAMPLRKKGSE"
BASE COUNT         4813 a         8836 c         8122 g         4559 t
ORIGIN      
        1 gtatgccgct ctcgggcgtg gcctcgacgg ccgctatgac ttccggtgcc cagccagtgc
       61 aggcgctgag cacctcctgt ttgctgatgc tcagagcggc atctgcggcg ttgccgtggt
      121 tgcaggttcc ccaccagtac gcctgcccat ctcccacatc gcacagccca aagcgcttgc
      181 ctcggcccca gtaatgcgcc acatagcctt cggtcatcac cgggtggctg agtggcgtta
      241 ccgccagcca cgcgatatac ccggatgggc gcgttggcgt ctcgcccagc atctgctgac
      301 ggatcactga gttcaaaccg tctgcgccaa tcagcagatc agcctcgtgc gtgctgccgt
      361 cttcgaacga cgccctcacc ccattcgggc gatgggcata acttacgcag cgcaaccctg
      421 ttgtcagggt atccggcgtg agctgctgcg ccagcgcgcg cagcagcata cggcgttgaa
      481 tattcacatt agggtggccc agctgttcgc cgatctcgtg gatgggcatg ctggtgatga
      541 gatcgccatt ttgctttttg aagaaaaagt gctgaatcac ctggcccgcc tgttccaccg
      601 gaacatgggc atcgatcgaa tgcagcgccg ccatggcatt ggccatcact gacacgccgg
      661 cgccaccggt acgtagcgcc tgggccgctt cgaagacctt gaccttccag ctactacata
      721 aagcggtagt cggccccatg tacgcgagtg acaatggcga gcaggtggcc cgcgtgaagt
      781 gatgatcggc cagaagtatt cacactggct cacgctgctc agacttgggc cgctccaatc
      841 cagggagatc tgatgcggtg agggcgcaga tctcacacta attcgcatgg caaactattt
      901 cttctttcac ccgcaggcga tccagttcgc ctgtgtagcg caaaaactgc cgctttttaa
      961 cctcgtctaa ttgccgtcat ccgccggacc cgatgccgca tccagccctc gcgagcctgc
     1021 tcacgaccac ccagaaaagg cagccccctc atcatctccc ctacaagctc gcttgatgga
     1081 atgggctcaa gttgagagca ttgccccgca caggggcaac gctaatagac caaaaacaga
     1141 ttaaggagag gccaacgccg taagcacaca gacaattgcc gccaccaggg gcgcctccct
     1201 caatcaatag cccgccccgc agtctcttgc ataaaaggca gcggcaacaa ggacaccaaa
     1261 ccacatccaa tcaaatacca ggcaggagcc aaattactcc cggaaagctg aatcagccag
     1321 gtggcaaaaa actgcgcgaa cccaccaaaa atagaaaccc caagacaata aacaaccgac
     1381 atcccggtag cgcgaatctc gcgcggaaac atctccggca gcatgacaat attaggtaca
     1441 gcggtaaaag caacaaacac cgccagcacg gcaacaacag acaacaacac agacacactc
     1501 ggcgtcgcat tgataatcat aaatgccgga tagacagccg caatcaacaa cacacgcgaa
     1561 acccacagaa cccgcttacg cccgacacga tcggacaacg cccccgcaaa cggcgaacag
     1621 atcaccgtca ccactgccgc ggtccaggca gcccatagcg ccgacgacat cggtaaatgc
     1681 agaatccgaa tcgcgtaagt ggacagatag aacagcacga tatagttcgc cgccgtacca
     1741 ccaatcgtcg tcaacactcc cgtcgtgatc acgcgcgaat gctcgcgaaa caacgtgcgc
     1801 acaggctgcg cggccgccac ctgcgccgcc ggaaccttgc cgtcctcaat gcccggcaag
     1861 gtctcatcca gatgacgccg gatataaatc ccaatcggcc ccatcgccat cccgatcacg
     1921 aacggcacgc gccagcccca gctctccaac gcggcagtgg acagcgccgc cgccagcgca
     1981 acacccagca gcgagccaca taccgtgttc aacccctggc tcacaaactg ccagcttccg
     2041 tagaatccac gcgtcttgtt gctgccatat tcaagcagca acgatgtcga cgcaccgatc
     2101 tcaccaccca gtgcgaaccc ctgaatcaaa cgcgcaagaa tcaccagcag cggcgcggcc
     2161 acaccgatcg ccgcgtaggt cggcgcgaac gcgataatcg ccgagccaag cgtcatcaac
     2221 cacaacgtaa gactcatcgc ggccttgcgt ccggcgcgat cggcataggc gccgagcacg
     2281 atgccgccca ccggccgcat gatgaaaccc acgccaaagg tgccgaccgc cagcatcagt
     2341 tgcgcaagct gcccttccac cgggaaatac agcttgccga tgatcgtcgc gaagaaactg
     2401 taaatcgtga aatcgaaaaa ctcgaggccg ttgccgagcg tgatcgcggc gatcgctttg
     2461 gcgtggctcg tacgtttggc cggctgcgcg cccgactcgc cagcgagatt caacgcgtca
     2521 gcgcctgcgg gttgtggtgt agcggaatag tccatctgtg tctccatggt gtccttcctg
     2581 ctgatgcgcg gctctgcggc cctgtgcgcc tgaaaacaat gcgcggcctg actggcctcg
     2641 cgcggcttcg ctggcctcgt tagccttgat ggccacgatg gccttcacag ccttcgtgac
     2701 cttcgcagcc ctgcatggcg tcagcccaac ccgacgcccc tcgcctcaca caagaaacgt
     2761 ctgcgcgagc cgcacccaat aagccgcgcc ggtcgcgagg cagtcgtcgt tgaagtcgta
     2821 gcccgggttg tggaccatgc aaccgccctc gccgtcgcca ttgccgatga tgaggtagct
     2881 gccagggcaa cgctcgagca ggaacgcgaa gtcttcactg cccgtcaggg gttgcatgtc
     2941 ggcaatcaaa ccgtcttcgc cgagccagtc gagcgcgact tgccgtgcga gaccggtcat
     3001 ctccgcatcg ttgacgagca ccggatagcg gcgctgataa tccacatgcg cccgcgcgcc
     3061 gaacacggcc gcctgcccgc acgcgaccgc cgtgatgcgt tcctgcaaat ggtcgcgaac
     3121 ctcgggcttc aacgcacgca cggacaggcg catctccgcg gtctcgggaa tcacgttcgg
     3181 cgcttcaccg gcgtggatcg cgccgaccgt gatgatcgcc atgtcgagcg gcgcgatatt
     3241 gcgcgacacg atggcctgca aggccagtac gatctgcgcg cacaccacta ccggatcgac
     3301 ggccttgtgc ggcaccgcgc cgtgaccgcc acggcctgtc accttgatga tcaccgtatc
     3361 cgacgaagcc atgaacgagc ccggcaggaa gccgaacttg ccagtcggaa aacccggcat
     3421 gttgtgcatc gcgaagaccg cgtcgcacgg gaatctgtcg aacaaaccgt cctcgagcat
     3481 tttcttcgcg ccggcctggc cctcttcagc gggctggaaa atcaggttca acgtaccgtc
     3541 gaaactcttt tcccgcgcga gatgtttggc ggccgccagc agcatcgcgg tgtggccgtc
     3601 atggccacat gcgtgcatct tgccagcgac ggtgctcgca tacggcaggc cggtggtttc
     3661 gtggatcggc agcgcgtcca tgtcggcgcg caggccgagt ttgcgcgtgc cgttgccgac
     3721 tttcaattgg ccgacaacac cggtctggcc cagcccgcga tgcacggtgt agccccactc
     3781 ctgcaagcgc tcggcgacca ggtcgccggt ggcgaattct tcgtaagcca gttcgggctg
     3841 cgcgtggatg cgacggcgca gcgcgatcat ttcgtcttcg agttcggcga tgcccgccgg
     3901 aatggccatg gtgttcacga tgtcgtctcc gtcgatgtat gcgtgaaccg agcatagcga
     3961 cgccgatttt ccagcggcag gcgtagaatc gttgcggcag caacctgcgg ttgccacctt
     4021 cctacaagct tgtcagatga aactgcatca actcagcact ttagcggcca ttgcggatac
     4081 cggcagtatc cgggccgctg cccggtcgct cgacctttcg ccggccgccg tcaccaaggc
     4141 catgcgcgaa ctggaagccg atctgcaagc gccattgctg gtgcgcggcg cgagcggcgt
     4201 cgcgttcacc gagttcggcc gcgcgctggt cgtgcatgcg cggctggtgc tcggccagtt
     4261 gcaccgcgcg caagcggaga tcgaggcgat gcgcggcgcg gcggccggca agctgtcgat
     4321 cggcgtcacg ccgtgggtgg cgctgacttt tctgccgccc acggtgcagc ggttccggcg
     4381 gcgcatgccg gaagtccagc tggaattctt cgagggtctg ttggcggtcg tgcaaccccg
     4441 cttgcgcgac ggcagcctcg acttctcgat tggccgtcct ccgcctgctt cgccgcaatc
     4501 ggagtttcac aacgtgccgc tcttttcgac gcactcggcg gtggtcgcgc gccgcgatca
     4561 tccgaaggcg ggttgccgca cgctgcttga actggaagac gccgagtggg tgttgaactg
     4621 ggacccggcc agccgtgagt cgatctcgga caatctgttc cgcaagcgcg gcatgcgcgt
     4681 gccgcatacc attcatctcg cgcattcgct cgcggtcgtg ctgggcctgc tcgcgcaaac
     4741 ggacatgctc agcatttttc cgtggccgct cgtcgaagtg agcatcgcga aggaaaatct
     4801 gtgggcgctc cccttgcgcg aaacggtgga cgaaacgatc gtcagcatca cgtcgcgccg
     4861 cggtgcgccg acaagtccag cggccgcgtg ctttctcgac tgtttgcgtg aagtaatcga
     4921 cgaaggagcg cgttcgcacg acccggaact gcgccgtctg tttcattcga tcgaactgct
     4981 gctgtaggga cggggctcga ttagatgacg gggcatgaga ctcggtggcg tggactcgga
     5041 ggcgtgatca aaattgtcga gcggagtcac aggcccactt cacccgtcct cttcaaacga
     5101 cgacgcacgc ggtcacccgc attcgctcgt tgccacatat cgcggttgta agctgaatcg
     5161 caggcacggc cactatgctt cgagtgcgta gtggcatgcc gccctgcgcc actccgtgcc
     5221 gaccatgaag cagactccag ccaacaacga agccgactac gacacacaat tgccgggcgc
     5281 gtcggcgcaa cgcggtagca atagcggtag cgccggcgat aacgacacag atagcgatag
     5341 caccagcggt agcggtagcg ccagcggtag cgccagcggt agcggtagcg gtagcggtaa
     5401 cgacagcacc aacgcctcgc tcgatccctc ccatcaccgc gccctcgccg atgcgcgccg
     5461 cactgacggc gacgaactgg gcgccgtcgc gcatctgatc gccgcccaca cactggacgc
     5521 ctacgccgcc gcggcacccg acgccagcgc cgcgaatctc tgcgatgtcg cgaccggcta
     5581 cttcatgaca ggcgatcacg tgatcgccgc gtactggtat cgcctcgttc tgacgctcga
     5641 tccgcgcgtc gcgattgcct gccagaacct ggccgccatc ctcgcaagcg ctggagacgt
     5701 ggccgaagct gatgcgtatc ggcagcgtgc ctaccggatt cagcgtgttt ttgtcgaggg
     5761 aagcggcgcg gagcagcgcc gcgtgctggt cctttgcgcg gcccacgctt ccggcaatgt
     5821 gccgttcgat gccctgctgc ccggcacgac ctgctgccgc atcaagtacg cgatcgacta
     5881 cgccgccgcc gaagaagacc aacagctgcc ggcctacgac ctcgtgttca acgcgattgg
     5941 cgatgcggat atcgcggccc cgctcgccgc gaggctcgcg cgcttcgtct cgcactgcgc
     6001 gcggcccgtg ctcaatccgc ccgccgccgt cgcccgaacc cagcggcacc gtaccgtcag
     6061 cctgctcgac ggcttgccgg acgtacaagc ggcaccttgt gtgcaaagcg atgccgcgcc
     6121 ggcttcacgc gccgcgcttg acaccctgct cgcacagagc ggtatcgact ttccggtcct
     6181 ggcgcgtccc gcggctacgc acggcggcga cgggctgacg ctgtgcgata gcctcgccgc
     6241 actcgaagcg tggcgctcgg cacaaaccgg cgttgcctat ctgacggcgt ttcgcgacta
     6301 tcgcagcgcc gacggcttct accgcaaata ccggatcatt tttgtcgacc gggagccgta
     6361 tccttaccat ctggcgattt cgccgcactg gatggtgcac tactactctg ccgagatgga
     6421 acagcatccg tggaagctgg aggaagaacg gcgtttcctc gacaatccgc atgcgatgct
     6481 cggcgagcgt gtcatgcgtg ccgtcgcggc catcggcagg cagctcgatc tcgactacgg
     6541 aggcatcgac ttgacggtgc tgcccggcgg acaggtgctt gtcttcgaag cgaacgcgac
     6601 catgctcacg cacttcgagc gtaagaccgg cgagctcgct cacaagaacc cttacgtgca
     6661 acgtatcgtc gatgcattcg aacggatgat gacgcgtcgc acggcgacat gaacgttgca
     6721 aatcgcgcgt tttcgcaata gacccggcgt gtcgacgcag atcaagcgat gtgcgttact
     6781 ggctggatag atgctgattc agatacggca cgaagccgtt ttgcgtcggt ccgatctgtt
     6841 cgacgcaatc atcatagtct gagccctgcg cgcgctcagg accatacatg ccacggcatt
     6901 gcgcatacag cgtgatggtc gctccgctgc ccttggccac gcgcgtggcc gaatagacgg
     6961 ccttgcccga gcggctcggc acgtcggtct cgatcgccac cgcgtccgcg cggacaatcg
     7021 acgtggacga atgcgcggcc acgtagtcct tcgtgagcgc ccaggccttg tcgcactgcg
     7081 tctgatccac gcatgcaatc gcggcgttac gctgcgcggc atcgagcaga ttttcggagc
     7141 gggtgaagtc acgggcctgg gcctcctgtt tttcggcgga catgcaaccg ctcagggcaa
     7201 gcgcggcgag cgcaatagcg atagttgttt tcacaggtca agatccgata gcgaattccg
     7261 gcgaagatta tagacgcctg cgagctgtgt tcgcagattg gaatggccat cgggaagtcg
     7321 ccggagcggc gacatctctc acgaccgctt aactttcatc atgcttgcgt ggcggcgtta
     7381 tcccgtctcc gcgccacgcc gccggatcgc attaccgacc ggccctcgcg ccgcctcaac
     7441 tcgcgtaacc caccacgccc ttgatctcca gaaagtcctc gagcccaaac tccccatact
     7501 cgcggccatt acccgattgc ttgaagcccc cgaacggcgc atcggggctg tagtccgcgt
     7561 agatcagata gatcgtgccg gtacgcaagc gcttcgccac cgcatgcgca tgctcgatcg
     7621 aggcggactg cacatacccc gcgagaccgt agagactgtc gttggccatg gcgatcgctt
     7681 cgtcttcgga gtcgtaaccg aggatcgaca acaccggtcc gaaaatctct tcgcgcgcga
     7741 tcgtcatggt cggcgtgacg ttgccgaata ccgtcgggcg cacgaagtag ccatccttca
     7801 atccttcagg acggcccggg ccaccggcca ccagcgtcgc gccctcttcg atgccgatgc
     7861 cgatcagccg ctggatcttg tcgaactgct gctcgctgat aacaggcccg agatcggtgc
     7921 ccttggcatc ggcgggaccg acgaccaacc cttcagatgt ctggacgcca ctgtaaagac
     7981 aatcggcgcg ggcaaaaata tcgcccgccc aacctttact tcgatctcgg gcgatgtaac
     8041 cttaaagccc cgtcaaataa ggctctacgg atgcaacgag aggccgtgcg aggactttcg
     8101 ttgcgccacg cggcgcgcaa acgtcgaagt gaatcgcgag aatccacgcg ctcaaaccgg
     8161 acttgcctga cgcgcctgac ggcgccgttt cgccgcggcg acggccggca gcggcaccgg
     8221 taccgcgccg gacatgccgt gctcgatcag aaaatcgcgg aacagcccgg cgacatgcgg
     8281 cagacgttta tcggacacgt gcatcacgta ccagtcgcgc tcgatcggat tggcgggcaa
     8341 gggcaacaac gccagcaagc cggcctgaaa ttccagcgag cacgcatgca gcgacagaaa
     8401 cgcgacgccc agacccgcct cgaccagttg cttgatcgcc tcgttgctcg agagttcgga
     8461 gccgatatgc agcttgtggc cggcgagccg aaacagattc tcgacggtcg agcgcgtgcc
     8521 cgacccccgc tcccgcacga acagattcgc actttgcaga tcgtccagcg acaggcgctt
     8581 tttcttcatc agcgcatggg cgggcgaggc gacgaacgcc atgggatgct tcgcgaaggc
     8641 ggtcgccacc gtgcgcaatt cgcgcggcgc gctgcccatc acggcaagat cgatctcgtg
     8701 cgtggccagc atgcggataa tgtcggcacg attgcccacc ttgaactgca ccttcacttg
     8761 cggacgcaat tcggtgaagc gcaccagaaa aggcgggatc agatattcgg cggtcgtgat
     8821 cgccccaatg cgcagcgtgc cgccctgctc gccatgcaac gcggcgagat catccgccgc
     8881 gccgttccat agatcgagga tttccagcgc atagcgcgcg aggatttcgc ccgccgctgt
     8941 cagttgcacg ccacgtccca ctcgctgcaa caacggcgcc cccgcagcct cttcgagcag
     9001 ccgcacctga agcgacaccg ccggttgcgt gagcttcagc tcttcggccg cgcgcgacac
     9061 gctgccgagc ttcgcgaccg tatgcaaggc tttcaattga cggaacgtgg cagcttttaa
     9121 cataagcgaa aacctatatg ttctgtataa acattaaatt gtgctgcggt tttagcggat
     9181 tctatactgg cccggacaga tgacgccgat gaataccggg aatagcccgg aacgctttca
     9241 ccggtcgagc agagataact tcacgaggat aggaatcatg gccgcaaccg atcagcaaac
     9301 accctctggc gcgcaatcca cgcaaccggg gttgcacaga gcgccggcag tagcgcatcg
     9361 cgtcgtaatc gttggcggcg gcgtcggcgg cctgggcctc gctacgcgcc tgtcggaaac
     9421 gctcggacgc gccggtcacg cgcagatcgt actggtcgac cgctggccaa cgcatttctg
     9481 gaaacccctg ctgcatgaag cggcgtcggg ccaactcgat ccggccacgc atcaactgca
     9541 atatgcggtg caggcgcaac ggcacggctt tgaattcgaa cagggcgaac tcgccgcgct
     9601 cgatcgaacc agccggcaca tcacattgaa cgcgctttac gacgccgacg gccgcgaaat
     9661 cctgccgtcg cggcagcttg cattcgatac gctggtgctg gcgatgggca gcgtcacgca
     9721 atatttcggc gtgcccggcg cggccgaaca cgccttgccg ctcgattcgg tcgcgcatgc
     9781 ggaagcgttt cgccgcaagc tgctcgacgc ctgcctgcgt gcgaaccatg cgcgccgggc
     9841 gcggaccgcg caggcggaca cgccggtgtc gatcaacatc atcggcgccg gcgcaacggg
     9901 cgtcgaactc gccgcggcgt tgcgcgatac agtgcgcctg ctgaagcact acagcccgtt
     9961 ttcactcgac cccgtgcgcg acttccgcat ccgtctgatc gaaagcagtg agcgcgtgct
    10021 gcccgccttg tccgaaacga tttcggcacg cgcgcaaacc atgctcggca gtctcggtgt
    10081 cgaagtgttg aacgcaacgc gcgtcaccga agtgcgcgcc gatgccgtgc tgaccagcca
    10141 gggcgcgccg ctcgccagcg acatcgccat ctggaccgcc ggcatcgcgg ggccgcccgt
    10201 gctgcgcaca ctggaccgca tcgcggtgaa tcgcaacgcg caggtgcagg tgaaccgcac
    10261 gctgcaatgc acgaacgacg cgaatatctt tggactcggc gattgcgcgg cctgcccttc
    10321 ttccgaccag gcggatgcgt ttctggcgcc gcgcgctcag gtcgcgcacc aacaggcaat
    10381 gtttctggcg cgtgcgctga agtgcaggat agcgggcgaa ccgttaccgg agttcgtcta
    10441 tcgcgacgcc ggcacgctgg tcggtttcgg ccgcgagggc acgatcggca gcttgagcaa
    10501 cagcttgctg acgcaaccgg tgttcgtcga cggctggctg gcgaccgccg tctacaagct
    10561 catctatcgc cgccatgtca tgacgttgac cggcttcgca cgcatggcgc tcgattcggc
    10621 gagccattgg ctgcgcagac gcgtgcatcc ggtgatccgg ttgcattgag taatttcgaa
    10681 caggcacgcg cgggacctcg ccaaaggtcc cttgcgtgcg catcacagcc cgcctgtcgc
    10741 actactcgat ccgaaatgca tccgccatcg agcggatcgc gcccggcagc ggacagggct
    10801 gcgggtactg gttgtccggc acatccatgc accccgccgc gcgcgcggca gagagttcac
    10861 gtctcacctc gtcgcgggtc tttccccgcg gcgcctgcag cgcctgcccc gcttgcgcga
    10921 cctgttgaac cgccggcacc gcgggagcgc gctggccctg caccggtggc ttctgcaaca
    10981 cctgctcgtg caatttataa aactcatgag gccccggcgt gggttcgcgc aacgggatag
    11041 tgtcgacggc caccgccgca ctcgcacagg tgcaactcag tacgcatcct gccaaccaca
    11101 tcgcatttcg cataatcgat tctccgtttg actgagtttc gatcttaggc ttggcgcgct
    11161 gcacaagcgt gagcgccgca tgacaagtcc gtcatccgct gcagtcatcg atgcactgcg
    11221 cgttcgagga ctgcatgacg gaattgtcat gttgttctca tcttgatgtc ggcgcgcatt
    11281 tatacgatgg atgctctcga cttcaaaccg ggaatcgcat gcgacgaatt caaccaatgc
    11341 gatgcgtggc aatacgggcg tcgaccgtaa gagcattcat gtcggatctc accagacggc
    11401 tccccaagtg caaacgcgag accctctccc tggccgcgag tctggtggct atcgcaatcg
    11461 gctccctcgc gttctggctg aaccagcccg ctcacctgtt cgacggtggc gcgttatctt
    11521 ccgaagccgc gacccaagcg caggtgagtc gcaaaagccc gccactcacg cacggcttgt
    11581 tatcgctcga gctgctttcc gccatgcagc gcgacgcgca aaagcagatg ccccatcgcg
    11641 acacgagcgc ccgaatgctc tgcgaacgga cgccctctac cgcagcttcg cctgcgtctc
    11701 gccatgttcg ctcacatagc acgcgcgcca gccgggcaat agcgtattga cggttcagcg
    11761 cgctgcctcg cgcggcgccg gcacggtcga gccgatgacc ggccgccctc tttcaactta
    11821 cacgaggcat ccagcgatga aagccttttt gaatcacacc gtggtataag tactgcatac
    11881 attcaggagg ccatgacaac tgcgcctacc cgatttcggc gaccacgcgt cttaaaatta
    11941 gccgccactt cggtatggtc cgatgcatgc ctgacgatat gaaacagaac attcaagcga
    12001 tccgtctttc ttcgatcgcg ccgccagcag tccacggcgc gatcgtccgc atccgcgcaa
    12061 ccgcatcggc agattcagtc ccgatagccg gactcacgcg cctcaacctg aacgcgatcg
    12121 cgcatccatg ctgcctcacc cggggcaagc aagccatgcg actcattggc gccagcgtgc
    12181 atgcacgtgc agcacttgca tccacgccag ggacccacct actcgctcac gcgtagcggc
    12241 gcttaccgcg tcaaaagcgg ccctactcgc gccctccgct tgccgcgccg cctacgcgac
    12301 acggcgcaga gagcgtctcc aggagaaaga ccatgcgaat tctgatcgtc gaagacgaag
    12361 cgaaaaccgg cacctacttg cgaaagggtc tgacagaagc ggggtttgtc gccgactggg
    12421 tgcaagacgg cgtgaccggt cagcatctgg cgttgaccga ggactacgac ctgatcatcc
    12481 tcgacgtgat gctccctggt ctggacggct gggccgtgct gaaaaacctg cgccacatac
    12541 gttccacccc ggtgttgctg cttaccgcac gcgatgatct ggacgacaag gtgcgcggcc
    12601 tcgaaatggg cggcgacgat tatctgatca agcccttcga ctttgccgaa ttactggcgc
    12661 gcgtgcgctc gatcttgcgg cgtggccatg cgcgcgacac ggcctcgctg cacgtcgcgg
    12721 acctggcgct cgacctgacg cgccgcaagg ccacacgtca aggcgacacg atcctgctga
    12781 ccgcgaagga atttgcgctg ctgtggctgc tgatgcgccg tcacggcgaa attctgccgc
    12841 gctcgaccat tgcctcgcag gtatgggaca tgaatttcga tagcgacacc aacgtggtcg
    12901 atgccgcgat ccggcgcgta cgcgcgaaag tcgacgacaa ttacgaaccg aagctgatcc
    12961 acacggtgcg cggcatgggg tacgtgctcg aagaacgcga cagcgcaccg cgatgaactg
    13021 gcgacagcgc tgctcaatcc tcgcgccggt ggcgcagtgg ctcgtgaggc cgctcgcgcc
    13081 gctcatgcca cgcaccttga gggtacggct ctcgatcctg ttcgcggtat ccacctcttt
    13141 attgctcgta ttcaacggcg tattgctgta tcacggtctg aaaacgcgcc tcgactcgac
    13201 ctcggcgcgc gagatgacag cgaccctctc cgcgctgcaa ctgagcctgc acaccatgcc
    13261 cggactggac gcggtccgcg ccgatcgtga gatctggtac gaccacctgc atggccacca
    13321 gaatctcgac attgccctgt tcgatgaggg cggcgcgccg ctcaatcgca ccagcggcta
    13381 ctggccaaat gccgaatcgc tcgcggtacg cgccggactc gcgcccgtcc aggtgacggt
    13441 agagggcatg cccatgcgtt ttctggtggc gatggcgccg ctcagcggcc aagccgatgc
    13501 gcgcgtgcgc gtggtcgtgc aatacgatgc cagcgccgag ggggcgttgc tcagcgccta
    13561 cgcgctcaac gtgtcgttcg tgagtgtgat cggcacgctg gtgaacgcac tgctggcgtg
    13621 gtggatcgcg cgtttcgggc tgcggccgct ggcccgcctg acggcacgcg ccgagcagat
    13681 ctccagcagc cgcctcgctc ggcccttgcc cgagcgtgac atgccgggcg aactcaagga
    13741 attgagccac gctttcaacc ggatgctggc gcgcttgcac gactccttta cacgtctcac
    13801 ccagttctca tccgatctcg cgcatgacat gcgcacaccg ctcaccaact tgctggccga
    13861 agcgcaggtc gcgctctcgc aaccccgtac cgccgacgat taccgcgcgg tgatcgagtc
    13921 gagcgtggac gaattccagc gcctctcgcg cctgatcgac agcatgctgt tcctcgcccg
    13981 cgccgatagc gcacagcgcc gcctggcgtt gcagacggtg gacgcgcgca gcgaggcgtt
    14041 acgggtcgcg ggctactacg aaagcatggc ggcggacgtc ggcgtggaga tcgtcgtgaa
    14101 cggcaatgcg aacttcgaag gcgacgtgct gctcgtgcag cgtgcactga gcaatctggt
    14161 ttcgaatgca ttggcccatg cgccgcgcgg cagcacggtc gaactgcgtt gcgtcgagcg
    14221 gctccacgac gtccagctat ccgtgtccga cagcggcgcc ggtattgccg agccgcatct
    14281 ggaaaggatc ttcgaccgct tctatcgggt cgacccggcg cgccacaact cggcgcgtgg
    14341 caccggtctc ggtcttgcca tcgtcaagtc gatcatgaac gagcaccacg gcgaatgcag
    14401 tgtcgagagc atgccgcacg tgcgcaccac attcagcttg cgttttccga agcagcccgt
    14461 agcgctgccg acaccaaaag tcccgcgtgg gagattccgc agacggcaca acaaataacg
    14521 gcgcatgacg taattatcat ctagcgatca ccgctgcctc atacctcccc atgcagaatg
    14581 agtcataccg gatacaactt cggcatgcca atcattcatg gagagaaaga tgaaatcaag
    14641 gcaaattgcg ctagccgcca ttctcatgag cgcgctgtcc acatccgctc tggcgagcca
    14701 cgacgacagc gtccgggcac ccggcgacgc agaccggatc gcgccgcctg cgctgactgg
    14761 catgcctgcg gcacagccac atcagctcgg aaagacacgc gcgcaagtcc gcaaggagct
    14821 catcgatgcg cgccgcgagg gaaccatccc aaccaccgag gccgattacc cgcagggcca
    14881 gcgaaccatc gccgcaaacc gcgcccgcaa ccggattttc gagaatttct ggacgggcaa
    14941 ggactgacgc tgacgcaaca acacaaggag caccgatgac ctacccccaa cgcatgctgg
    15001 tcgaaaacct cgcgtggtcc gccgagatca gcaaccgcga tcccgcctac tttcagcgcc
    15061 tcgcgagcga ccagcggccc aacgtgttgt ggctcggctg ctccgacagc cgcgtccccg
    15121 aagccatcac gcgagcgatg cccggcgaga tcttcgtaca ccgcaatatc gctaacctgg
    15181 tgagcgaaca cgacgacagt ctcgcgagcg tgctcgaata tgcgatcgtc gtgctgcgcg
    15241 tgcagcacat catcgtctgc ggccatcatt gctgcggcgg tgtgcaagcg gcgttgcggc
    15301 cgccggcagg cgcgttgccg aacgtcaatc gccgcatcga cccgattcgc cggctggccg
    15361 cgctgcatgc agcggaactc cgtctgctgg atgcttccga cgaacgcgcc gatcggctcg
    15421 ccgagctcaa cgtgctggcc caggtgcagg cgctgcacgc gatgccgctg attcaaaacg
    15481 cgcaacggcc cgtgcagatt cacggctgga tcttttcgat gcacgacggc cgcctgaagg
    15541 cgttggccga cccgcagtgg gcgctcagcg acgccgacga cgtccacgag gcgaccggct
    15601 gactacagta gccgcatgcg ccgtgcgatc ggcgcatgcg aacagcaacg caatcaacgc
    15661 gcaacccacg tttccgatgc ggcgctgcgc ctatggcgcg agcgccgcgc ctctcatgca
    15721 gttcgccccc ttcgaggtta gcctcccgat gatgcaagct cacgccaccc cacctcatga
    15781 ccgttcacct cgcgcctgcc cgcccgcggc ctggcgcacc gatctattgg cgggcatcag
    15841 cgtattcctg atcgcgctgc cgctgtgtct gggcattgcg accgcctccg gcgtggcgcc
    15901 gtttgccggc attctcgccg gcgtgatcgg cgggctcgtg gtggcgctgc tgagcggctc
    15961 gcgcctcagc gtaagcggcc cggcagccgg tctggtaatg atcgtcgtgc aggcgcttgc
    16021 cacgctgggc agtttctccg cgtttctgac cgcgctggtg ctcgcgggcg ccttgcaatt
    16081 gatattcggt ttcctgcgcg ccggtaaatt ggccagttac gtgccgcagc cggtgatcaa
    16141 cggcatgctc gccgcgatcg gtatcctgct gatcgtgaat cagttgccgc tggcggcagg
    16201 cttcgcgaat ggcgacattt ccgtcgcgat gccgttgctc gcgctcgcgt cgctggccat
    16261 tctcctcgcg tgggaaacac ccgcattacg cgccttcgcc ctcgtccgcg cgcttcccgc
    16321 gccgctcgcc gtcgtgtctt tcggcattgc cgcaaccgcg ctcgtcgata cgttcgcgcc
    16381 gggcgtggcg ctcgcgtcct cacagcgcgt cgcgatgccg ccactcggtt cgtgggatgc
    16441 gctgcgtcag gcattcacct tcccggatct cgcgcaatgg ctcaatgccg atgtatggcg
    16501 cgtggcggtg gtgatcgcga tcgtcgcgag tctggagagc ttgctgagtc tcgaggcgct
    16561 cgaacagatc gacccggagc atcgaaaagc ggcgcccgat cgcgaattga aggcccaagg
    16621 cgtcggcaat ctgcttgccg gcctgctcgg cgccttgccg ctcaccgccg tggtggtgcg
    16681 cagcgccgcc aacgtgcatg cgggcgcgcg cggccgcctg tcgtgcgtga cacatggcgt
    16741 cgtgttgctg atcgcctgtc tcgcgttgac ccgcgtgatc aatatgattc cgctcgccag
    16801 cctcgcggcg atcctgattc ataccggcta caaactcgcc aggccgcaat tattcgtgtc
    16861 gatgagtcag gcgggcttcg cgcagttcgt gccattcatc ggcacgatcg tgggcgtgct
    16921 cgcgaccgat ctcctgatcg gcatcgcgat cggtttcgcc ctgagcgtcg tgctggtggt
    16981 cgaacgcact atccgccgcg tgctgagttt cacgcgcgac ggcaaccact atctgctgac
    17041 cgtgcgcaag agcgcgacct tcttctgcac accgcaactc aagcactatc tcgggcaggt
    17101 tccgtccggc gcgacgttga ttctcgatgc gacccacgcc gatcatatcg accacgacgt
    17161 gcgtcaggtg gtcgacagtt atgcggcgcg agcgagggct agcggcgtgc gtatcgagta
    17221 caagcagtgg ccgcccgcgc accgctaacg tgagccgcaa gcgggtgact tgccagggcg
    17281 gcgctcaatc gagaaactcc gccgcccgct tgcgcagcat cgcgccgaaa tcgtcgagcc
    17341 agccgatcga caagcgccag ctctgccagt acagatcgac atcgatatgc ttgccgggcg
    17401 ccatgtccac cagttcgccg ctcgccagat gcggcgcgat catccgttcc gggcacatgc
    17461 cccagcccag tcccatcacg caagcgcgca aaaagccggc gacgtgcgga atccagtgca
    17521 gtggcggatc gagttccgcc cgcgtgacgc gccgcatgaa gcgcttctgc aactgatcct
    17581 tcggattgaa atcaacgcac ggcgcgcgcc gcaacgtgtc gcgcgtgacg ccgtcggcaa
    17641 aatggcgctc acgaaattcc ggcgtgcaga ccgccagata gcgcatccgg ccgagccgtg
    17701 tcgagcggca gccctgcacc ggctccgctt gcgtggtgac cgcgccttgc acgctgccgt
    17761 cgcgaatccg ctgtgcggtg tggtcctggt cgtcgaccac cagatcgagc agcatctccc
    17821 gttcgacgca gaagcgcgcc accgcgtcga tgaaccaggt gcccacgctg tcgtcgttga
    17881 cggccacgcg cagcgccggc cacggttcca ccagggtgcc gggcagtgcc gggaggcggc
    17941 cggcgagttc cgcctccagc aactgcacgc gttcggtgtg gcggcacagc agcgcgcccg
    18001 aagtggtcgc gacacacggc tggccgcgct tcacgagcac gctgcccacc cgctcctcca
    18061 gcagcttgac gcgctgggag acggctgagg gcgtcacgtt cagttcgctg gccgctcggt
    18121 cgaacgaacc gtgccgcacc acggcggcca gcgcgtcgag caacgcatag tcgagcatta
    18181 gcgttcctta atcggcatta agtaaattag cttctcttat gagatgcaaa accgcagact
    18241 agcctcgttc gcttttcccg tctactggat cgattatgaa ttggctgtct ttttcccacg
    18301 gcgccgcctt gtgcgcgtcg ctgatcgtca cgatcggcgc gcagaacgcg tttgtgctcc
    18361 gtcagggcat catgcgctcg cacgtcggca agatcgtcgc gctgtgcgcg ctgtcggact
    18421 tcatcctgat cggcgcgggc gtggggggcg cgtcggtgct ggtggaacgc tatccggtgt
    18481 tcgtccacgc catgctgtat gtcgggctcg cttatctggc gtggttcggc atcaacgcgc
    18541 tgcgccgtgc ggtgcgcccg gcccatgcag tgctggacac cgatgcgagc ggcgcggcgc
    18601 ccggcacgag cacggcaccc gtgcaacgtg cgatgcccat cgtcctgatg accctggcct
    18661 tcacgtggct caacccgcac gtgtatctcg acaccttcct gctgatcggc acggctggcg
    18721 cgcgcgaacc ggacggcgca cgggtggcgt tcgcactggg tgcgatggcg gtgagcgggg
    18781 tctggtttat cggtctgggt tacggcgccc gtgcactggc gccgctgttt cgccgggcta
    18841 gcgcatggcg cgtgctggat ggcgcgatcg gcagcatggt gctgctgctg gcggtgacgc
    18901 agttgcggtg atcagagcgc gccgcctgag ggcgcgcgct gcctgagaac gcaccaaccg
    18961 gcgcgttgtc gagcgagcgc ctagtcgaag tcgaccgctg ccgcttgcac ggtgtcgtgc
    19021 aaagcggacg cagtggatgc cgcagacacc gtggcctcgt cgagcggcac gagcttcatc
    19081 aacctggcga gcaccggcca tttcaccagc gacagctgat acgactcccg cgcctgctca
    19141 tcgaagtacc tggccggatc gacttcaggc aagcggcgcc caaggccggt gatatcgtca
    19201 accggccagc cactcgcctg catcttcgct tcaggaccgg ctgctgtcat ttgccgtcca
    19261 cccatacgcc atccggcgtc ttgaccacat agccggacgc cgtcttcatc gtcacggtcc
    19321 cttcgttctc gccgaccatc tgcgtttgcg gcagattggc tgcgtattgc ggcaaggtca
    19381 cacccggttt gaacggactg ttcgacacca gattcgacag caattgcgcg accgcgagaa
    19441 aactcgacgg attgtcgatc accgtggtcg gcccatggtt gccctggaag ccgaccagcc
    19501 gcacgccggc cggtccatgc acgatgcgcg gcgtcggaat ctcgcgcagc ccggccacct
    19561 ggttcgcatc cccgcgcagc gccgcgccat gctccggcac gaacacgatc acggcgcggc
    19621 gcccggaggc cgcgatcaga tccgcaaaac ggtcgaagtc gctcatcagc ttgttcacgc
    19681 gttgcgggta cgaatcgata ctcgacagat tactgttcgg cagacggtta ccgtcgtgca
    19741 ggctgatcgt gttgtagtac agcgcgaccg gaccgggcgt attcgcacgc tgcgcatacc
    19801 agttggcgag cgtcgcgtaa tcgtcgcgaa tggccgagcc gtcgaaggca tgcatcgcga
    19861 tcggcatgct cgtgttcgag atcatcggca cgttcggcac gccggtgttg tcgtgcacca
    19921 gttgcaggaa gttgtcgaag tggccgtcgt gattcagcat cgtctgcggc gtgtagcccg
    19981 cctgcgccag gtcggcgagc aggtggcatt gctgcggcgc atccttgtaa aggtccgcgt
    20041 gcgcttcctg tccgcaactc gcccgcagca cgcggatcgc ggccggcccg ctgtagctgg
    20101 cggcggtgct gaagttggtg aacagatagt cgaagtggct cagcatcgga ttgttgcgcg
    20161 ccttcgccgc gtcgaggtcg tcccacgaca acgaacagat atgcagcacg atcacgtcga
    20221 actgcgtgtt cgcatcggcg ccgagatgac cgaaggtgac ttgccgttgc gactcctggg
    20281 tgcggaaggc cgacagcgcc gcgttatggt cgagtggctg tgcggcaagc gtattcggcg
    20341 acgcagcggt ggccggtccg ctctgaagtc gcgcatacac cgcgctgccc tgttgccaca
    20401 acggaatcgc gaccagcgcg atcagcacga acgtcgccac acgcagccag cggttgacga
    20461 tcagatagcc gatcagcgcg ccgagcgcgg tgagcagcag catcggcggc aagaaacgct
    20521 gcgcgagttc gagccagtag ccgaaactga agctggtgag gtcgccgaat tcttcgatca
    20581 gacgcgagaa cggcggcacg ttcgcttcgt agtacatcag cggaatgccg agcgcgagac
    20641 cgaccaggtt gcgcagcacc cgccacccgc gatggcgcag cgtcgcgctc gccgccagcg
    20701 ccagagcgaa cccgatgttg acgatccaca ccggctgcag atgtccgccg gtaaacagat
    20761 acagcttgat gatgaaataa agattccaca gcgtcattga cgaagctctc tcattggccg
    20821 gccgcatgcg ccagcggtat taataacgtg tacggtccag cgcgagcagc gactgaaccg
    20881 cgcgcgctgc gccgttcagc aaccgcgaat aaccctcgat gcccatcgtc agcacgtcct
    20941 tcaggccgcg cagcggcgtg tcctcgatcg tctcttcgtc ccagttgagc caggcgtcgg
    21001 cgcggccgaa cgtgcattgc accatctgcc gctcctgttc gagcgtcagg ccgtcgaaac
    21061 gcacgccgag gtgcgagccc acaaagcggc tcacgtgcac cggaaaatgg aactgccggt
    21121 cgccccggct caggcacacg ccgaggctgt cgccgagtgc gagcttcaac gccggatcga
    21181 cttcgagacc gagaccgccg gtcgaatagt cgctggtgat gcacgccacc gtcgtgccgt
    21241 ccgcgagcag cagcgtcgcc gaggcgcgca tcgcgatacg gtgagtgacg cgcacctgct
    21301 tcgcctcgcg cgccacgctt gcggccgcac cgagcatcac gaggttgtag agggtccaga
    21361 acacgttcat caggatcgtc gcggtttcgc cgcccgggcc gagcgtcagc cggtacacgc
    21421 ccgccagcag cgcgctggcg ttcagcgcca gcagcaccag atacggcttc gacaccgccc
    21481 agtcgaagta gccttcggcg atgcggccgc ccttgtcggt cacattgaac ttgccgtgct
    21541 tcgggctgaa aaacgcgatc gtggtcggca gcgcgatgta ccacgcgagc accgattcgt
    21601 agacttcggc ccagaacgaa tggcgaaatt tgccctgcat ccgcgagttg gcgatgttgg
    21661 cgagcacgat gtaaggcagc acgaagcccg cgatggtgat cgcggacgca ttgatgaagt
    21721 acatctggaa gaacaggtac gccatcggca tggtcaggaa cacgagccgt gggacgccgt
    21781 agaagaaatg cagcatcgcg ttgccgtagc acaggcgctg gaacagaccg agtccgcggc
    21841 cgagaaacgg attgtcgatg cggaaaatct gcgccatgcc gcgcgcccaa cgggtgcgtt
    21901 gcttgatgtg gccggcgaga ctctcggtgg cgaggccggc tgcctgcacg gtcggcagat
    21961 acgcggtggt atagccgcgc cggtgcagtt tgagcgcggt gtgcgcgtct tccgtgaccg
    22021 tttcgaccgc gatgccgccg acttcgacga gcgccgcgcg tttgatgatc gcgcacgagc
    22081 cacagaagaa cgatgcgttc cacagatcgt tgccggactg tacgagcccg tagaacagat
    22141 tgccttcatt gggcacacgg cggaaggtgc cgagattgcg ctcgaacgga tcgggcgaga
    22201 agaaatgatg cggcgtctgc accatcgcgc agcgggcgtc gcgcaggaat gtacccatcg
    22261 tggtttgcag aaacgagcgg gtcggcacgt ggtcgcaatc gaagatcgca atgtaatcgc
    22321 cgtgcgtttt ctcgagcgcg cggttgatgt tccccgcttt cgcatgctgg ttgtcgctac
    22381 gcgtgaggta gccgatgccg gcatcgcgtg cgaaattctt gaattcctcg cgagtgccgt
    22441 cgtcgagcag atagacccgc aacctgtccg cgggccagtc gataccctgg gcggcgaaca
    22501 cggtcggccg caccaccgtc aacggttcgt tataggtcgg aatatagatg tcgacggtgg
    22561 gccagctcga ggtgtccgcg ggcagttcgg caaccgtgcg ggcgagcggc cacgcggtct
    22621 gcacgaagcc cagcagcaga atgagccagg tgtacgcttc ggcgccgaac agcaggtagc
    22681 cgagaatcgc ctcgcccggc gtctggaacg acagggtttg cgtgacgcgc caccagacgt
    22741 agcgcaccat cgcgagcaag gccagggtcg tcagaatgag cacgggcatg cggccaggga
    22801 agcggcgtac gacgagcgcc acgaccgcga gcaccgcgaa gaattcgaac tggccttccg
    22861 cctgcaacgg cgaggtgccg atcatggacc acaccacggc gcccgccacg acgatgaacg
    22921 gcagcagcca gcgaatgtgg ccgatgcgct gcgtgccggc ttcgagctgc acgccccagc
    22981 gttcccacgg caggctcggc agcatcgctt cgatgcgccg ctgcacgcgc cgccagcgca
    23041 gcagcaccgg caccagccag cggtcgatcc acgcgagcac ggcgcgcgct ttggccgcgc
    23101 ccgtctgtgc gcgcggcttc ggcgcgcgga tgaacgcgcg ccacaaccac gcccgcagcc
    23161 gatgcggttc gagcacgccc caatgcgcgg ccagatgcag cacggcggca cgaatcccca
    23221 ggcgcgcgag atccgcctgg ccggcagccg gcggctggaa gaacaggcgc acgaaccagt
    23281 cgagcgccgt gcgctcggcg ggcagtccga ggccgcgcgc gagccagtcg aacgcgcgtt
    23341 ggcggcgcgt gcgctcgctg cggccgacgc aggcgggtcc gctcatggtc gcccatccac
    23401 acggcgcgcg tttgccgccg gcgcatcagg cagcgtcgcg gcaagccacg catgcagcca
    23461 attggcgagg ccttgcagat cgtgcgaggc ctgcgaatgc ggtgcctcgt cgaatagcca
    23521 cgtgccgcgc gcgaacgatt cgggcagtgc cgcgtccaga tgcacgcgct gagcgagcgg
    23581 caagccgtcg ccgagcgcgg cgcccagcat cgccagcgcg tcgcgttgca tgtcgcgcgc
    23641 cggattcagc cggttgacga tgattcgcag cgtcgcgccg ccgcgccgca acgcgtcgag
    23701 ccgctcgacc agggtcgcgc aggcggccgg ttcaggcggc gtgacgcaca gcacgagatc
    23761 ggcgctctgt acagcctgct cgccttgctg cgacggataa cgggccgtgt cgacgagcac
    23821 gacgccctcg cgcggcaaat cgagttgcgc gagggcgttg gcgagccacc gcggttcggc
    23881 ggcgaggcgc gcatcgcagc gcgcgctttg ttcgagcgac accgcgccgt acggcacgaa
    23941 cagcacgccc tcgctgttgc gccacgtgtg cgcatgccac gctgcggttt cgtcgagcat
    24001 ggcttgcgcg aggccggccg gtgcgaggca atcgagaccg aggtgcgcgc cgagcatatt
    24061 ctgcgggtcg aaatcgagcg ccaccacggg acggccgcgc cgcgcgagca gcacggcgag
    24121 cgccgcgctc agggtcgtgc ggccggtgcc gccggcggtg gagacgacag caatcgtctt
    24181 catgaggtgc ctccaggggc acgctcggcc tcgggcaaca ggcgcgcgcg caatgcgatc
    24241 ggctcgatgt cgcaaggcac cgcatcgcgc ggatcgaagt gtcgtatcgc cgcgagaccg
    24301 cgacgcgagc cgccgcgctg ccacacaatc cacacgggaa tcgcgagaac cagacccgcg
    24361 acaaaacaga tcaacaggaa ttccattact cgctcccctt cttgcgcagc ggcatcgcgc
    24421 agggttcggc gcggcgcggc ggcgcgaccg cgcggggcgt gggctgcgcc ggcgcaattg
    24481 gtccggatgc ctcggcgggc cctgctttgt gcccggcttc gtgcccggct tcgtgccctg
    24541 ctttttgccc tgctttttgc cctgctttct gctcgacttc gtggccggct tcatgttccg
    24601 ctttgtgccc ggcttgctgg ctcgccacct ggccggtctt ctgtgcaagc tcctgcgtcg
    24661 cttcctcgcg cgccgcgtca agcgcttgct ccaccgccgc gagctgctca accgtcgaca
    24721 tcccgtcttc agccggcaag accgtaccgg cgtgcgcggc tgcgcccggc gcagcagttc
    24781 gactacgcaa cggcgtagcg aacagatcgc tgtagtcggc gatcggcgcg cgccggctcg
    24841 ccgtggcaag cgccttcaat tcatcgccga tgctctgctc cgcaagatgg acgatgccgt
    24901 ccgcaatcct gttgaccggc acgttgaaga tgcgggccag cgcggcgtcc gcgtctgcca
    24961 gccggcacgc gaacaggaac acgtacaggt gcgaggcatc ggcggtgaaa atgtcgccgg
    25021 cacggcgcgg cacacagctt ttcagggctt cgacgtgcgc gcattgcggc agcagcgtca
    25081 gcttgacgag cacgtgcggc agttgcagca cggcactgcg ttcgagcacc gcgcgcaccc
    25141 gctcgcagaa cgtaccggcc ggcagatacc ccagcacgtc ctcgctcagc gcggcgctga
    25201 gcgcggtgcg atagtcagcg gcgaccgccc gcgtgtgcag ttgtccctgc aacgactgca
    25261 gcagcgactg catccgcgag aacggcagat cgcgacccag caccaggttg gcgccgagcg
    25321 tcagcaccag cagttcgtat tgatggcgca gcacttcgcc ccgttcggcg acgacgatct
    25381 tcaacgcgcg cccggcctgg cggcgcagcg catggatcgc cgcgcacagc ccttcgagtt
    25441 gcgcaccgct agcgtaatcg agcacgatgg tcgccgcctg cgcatcgacg caggccgcca
    25501 gcacggcctg ctgatcggcc agcacttccc agttcggcgg cacccacgcc tcatgtccgc
    25561 gcaccaccgc gcggctgact atcacccgct gttcgtcgcg cgccagcagt ccatcgccgc
    25621 gtgcggcggc gtcgctgagc ggatcgggcg cgacgctcag gcggccgtta tcggtaaagc
    25681 gcagcgagcg cacctcgccg gtgacgagcg tgcggtccga gcgccagaaa tccacctgcc
    25741 acagcaactc gccgtgggtg cgctccatcc gcgcgacgcc cgcgcaggcc gcgtgaaatt
    25801 cgttgcggcc aagttgcgcc gctgcgctgt cgagggtggg ttcgatgaac agtgaaccgg
    25861 cagcggcgtc aatgtccgtg tcggcatccg cattgacgtc cgcattgacg tcggattgcg
    25921 aacccagcag caacaccacg gtgatgtcgc gcaccgcgca ccagttcgcg agcatccggc
    25981 cttcacgcgc gagtgccgcc ggatcgtccc agttgaacca gcgctcggcg ccttcgacga
    26041 aatacagcga gcgcgaacgg aagccgaagc gcttgagcgc gcgcaagccg ccaaacagct
    26101 tggcaagcgg gaccggcacc accccgtggg ggcctgcgtc gccgggaccg gccggtggca
    26161 tcgcgaggac gctcaggtta cgcggccagc cggaggccgg cgcgtggctg ctgaaaccga
    26221 gttcgcgcag acgtttggcg acctgcgcgc gagcgcgtgc cagcaccacg gtgacgtcgc
    26281 gcgcctgcgc ctcgcgcgcg ctttcccaca gcagcgcgtc ggcgcctggc
//
LOCUS       DATASH010001103         5914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_783773,
            whole genome shotgun sequence.
ACCESSION   DATASH010001103 DATASH010000000
VERSION     DATASH010001103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5914
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783773"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..723
                     /locus_tag="VHV99_29255"
     CDS             88..723
                     /locus_tag="VHV99_29255"
                     /inference="COORDINATES: protein motif:HMM:NF004113.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX3639498.1"
                     /translation="MQAQATRATHPARAAEPRALSERAPDSERDPDAELLARVARQDP
                     AAVRSLVARKLPRLVALATRLLGDRMEAEDVAQEVFVRIWKQASRWREGEAKVDTWVH
                     RVALNLCYDRLRGRREVPHEDIPDAIDPVALPDATLETRARDERVRAALAALPARQRE
                     ALVLNYYQEMSNIDAAALMGITVDALESLLARARRNLRAQLAGSGLSKDKS"
     gene            720..1139
                     /locus_tag="VHV99_29260"
     CDS             720..1139
                     /locus_tag="VHV99_29260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639499.1"
                     /translation="MTPERFHQIVEAYGADPRRWPQQERAAAQRWAAAHRVEADTALA
                     AALGIDAWLAADKVEPPGMALQQRIIDSAPVRRPSAPRRRLLWSGAAVAGVGLLGGVA
                     GAFAVSFFVLAGTVPQAPESSYLTSSFGGASADWSGE"
     gene            1136..1678
                     /locus_tag="VHV99_29265"
     CDS             1136..1678
                     /locus_tag="VHV99_29265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HEX3639500.1"
                     /translation="MNGRSWKIVLVASLVLNVFLLGAIVGGAYQWFAARAAGGVVVAQ
                     QRTALRFAAEPLPAERQKAFVDGLKNARRDGREFAREGRESRREVLRLLAAPQFDRAA
                     LDAALARTREADSSLRAKVEGSVADFAATLSPEQRVEFAESLKRRGQWREPQLAVNTN
                     KPAKSGNAAASDASSDASGE"
     gene            1754..3361
                     /locus_tag="VHV99_29270"
     CDS             1754..3361
                     /locus_tag="VHV99_29270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HEX3639501.1"
                     /translation="MAASPNMNPAAIALNRFGLGARADDTPPADPKGWLLAQFEQYQP
                     RPAAWASQPDSVALSTELAQQRMQMNQLNQQNQQNPQNPQNVSERGAASQGSAQASTQ
                     ANGQGGTQTNPQTTGKTNPQTTGQGATETTAQTAKQAERKALRGEILDMYRSSVNALV
                     ASALTTQTPFIERLVHFWANHFAVSTEKPGVAALAGSFEAEAIRPHVLGRFEDMLVAV
                     ERHPAMQLFLDQTRSVGPDSMAAMRAAQRNPDIKRGLNENLAREIMELHTLGVRSGYN
                     QDDVTEFARALTGWSLAGNPGAAGNPRNTGGQTMPPNAAPGTFVFRAALHEPGARTVM
                     GRRYDQPGEEQALAILHDLASAPATAQHIGGKLARHFVADNPPPDVSGRLASAFARSG
                     GDLPTVYRTLIDMREAWSPVAVKFKTPWEWAISSMRGLGWQDLGNLQAAPILTQLGQP
                     VWRPGSPAGYDDIAASWAAPDALVRRVEVAQRFASRVGDRLDARSLGPTLLAGSLSAP
                     TATAVSRAESASTAIALLLVSPDFQRR"
     gene            3367..4515
                     /locus_tag="VHV99_29275"
     CDS             3367..4515
                     /locus_tag="VHV99_29275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX3639502.1"
                     /translation="MLSRRKFLSVAAAGAGAILVSPRIAFASVATDRRFVFVIQRGAA
                     DGLNIVVPYAEPAYATLRGALAIDASNATRLDGTFALHPSLARTAAMYADHQALFVHA
                     VASPYRDRSHFDGQNVLETGGTSPYQMKDGWLNRLVASMPATRENAIAFAPTVPMALR
                     GKAAVTSYAPSGLPQAPDDLLMRVSQLYDQDAQLRPLWQSAMTARGLAGDAGARQDPA
                     SLGKLAAGFLSRDDGPRIAMIETAGWDTHTAQNARLANQLKALDTMLAALRDGMGPLW
                     RKTTVVVATEFGRTAAANGTGGTDHGTGSVAMVLGGSVAGGRVLTDWPGLKPGELYEA
                     RDLKPTMSLDALIAGAASESLGLDPQRTAVTLFGQTAGTQPMTGLVRA"
     gene            complement(4748..5113)
                     /locus_tag="VHV99_29280"
     CDS             complement(4748..5113)
                     /locus_tag="VHV99_29280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014973409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX3639503.1"
                     /translation="MKTVNLLKALGIALCVATVSSAYAQSSDAMAAPAASASPNPKAM
                     KSADRKLGLSVRKALSKTQGFDVSNVFVRARGGAVTLTGSVPDGAQIPQAEEVAKNVA
                     GVKSVNNKLTLGTQGGGGG"
BASE COUNT         1036 a         1888 c         1978 g         1012 t
ORIGIN      
        1 gatgcaggcg cgaactcaac gtgcggtgcc ttgaaaggag tggcggaaag tgaatgacgc
       61 tcgtccgacg acgcgatccg tccggccatc caggcccagg caacccgcgc cacgcacccg
      121 gcgcgcgctg cggagccgcg cgccttgagc gaacgcgctc ccgatagcga acgcgatccc
      181 gacgccgaac tgctggcgcg ggttgctcgc caggacccgg ctgccgtccg ctcgctcgtc
      241 gcgcgcaagc tgccgcgtct cgtcgcgctc gccacgcgcc tgctgggcga tcgcatggaa
      301 gcggaagacg tggcgcagga ggtcttcgtg cgcatctgga aacaggcttc gcgctggcgc
      361 gaaggtgagg cgaaggtcga tacgtgggtg catcgcgtcg cgctcaatct ttgctatgac
      421 cgcttgcgtg gccgccgcga agtcccgcac gaggacatac ccgacgcgat cgacccggtc
      481 gcattgcccg acgccaccct cgaaacgcgc gcccgggacg agcgcgttcg cgcggcgctc
      541 gctgccttgc cggcacggca gcgcgaggcg ctggtgctca attactacca ggaaatgtcg
      601 aacatcgacg ctgccgccct gatgggcatc acggtcgatg cattggaaag tctgctggca
      661 cgcgcgcgcc gcaatctgcg cgctcaactg gccggcagcg gccttagcaa ggacaagtca
      721 tgacgcccga aagattccat cagatcgtcg aagcctacgg cgccgatcca cgccgctggc
      781 ctcaacagga gcgcgcggcg gcgcagcgct gggctgcggc acatcgtgtc gaggccgata
      841 cggcgctggc ggccgcgctc ggcatcgacg cgtggctcgc ggccgataag gtcgagccgc
      901 cgggcatggc gctgcagcag cgcatcatcg acagcgcgcc ggtacggcgc ccgagcgcgc
      961 cacgccggcg attgttatgg tcgggcgcgg cggtggccgg cgtcggactg ctgggcggcg
     1021 tggccggcgc ttttgcggtg tcgtttttcg tattggcggg gacggttccg caagcgcctg
     1081 aatcgtctta tttaacttcg agcttcggtg gagcgtccgc cgattggagc ggcgaatgaa
     1141 cggccgatca tggaaaatcg tgctggtggc ttcgctggta ctcaacgtgt ttttgctggg
     1201 cgcgattgtc ggcggtgctt atcaatggtt tgcagcgcgg gccgcgggtg gcgtggtggt
     1261 ggcgcagcag cgcacggcgc tgcgcttcgc tgccgaaccg ctgccggccg agcgtcagaa
     1321 ggcctttgtc gacggtttga agaatgcgcg ccgcgacggg cgggaatttg cgcgcgaggg
     1381 tcgcgaaagc cgtcgcgagg tgttgcgcct gctggccgcg ccgcagttcg atcgggcggc
     1441 gctggatgcc gcgctggctc gcacgcgcga ggccgacagc agtctgcgcg cgaaagtgga
     1501 gggcagtgtg gcggattttg cggccacgct gtcgcccgaa cagcgtgttg agttcgccga
     1561 gagcctgaag cggcgcggac agtggcgtga gccgcaactg gccgtgaata cgaacaagcc
     1621 ggcgaagtcg ggcaatgcgg ccgcgagtga cgcatcgagt gacgcttcgg gcgagtaaaa
     1681 aaatgctgaa cgggcgcgac ggatttcatc gtggcggtcg tttaaagaca tatccaacgc
     1741 cgaagaggat ggtatggctg cctcaccgaa catgaatccc gcggcgattg cgctgaaccg
     1801 cttcggcctg ggcgcgcgtg ccgacgacac gccgcccgcc gacccgaaag gctggctgct
     1861 cgcgcagttc gagcagtacc agccgcggcc cgccgcgtgg gcgagccagc ctgattcagt
     1921 cgcgctgtcc accgagctgg cacagcagcg catgcagatg aaccagttga accagcagaa
     1981 ccagcagaat ccgcagaatc cgcaaaacgt cagcgaacgc ggggcagcta gtcaggggag
     2041 tgcgcaggcc agcacgcaag ccaacggaca aggcggcacg caaaccaacc cgcaaaccac
     2101 cggaaaaacc aacccgcaaa ccaccggaca aggcgcaaca gaaaccaccg cccaaactgc
     2161 taagcaagcc gaaagaaaag cgctgcgcgg cgaaatcctc gatatgtacc gctcgtcggt
     2221 gaacgcgctg gtcgccagcg cgttgaccac gcaaacccct ttcatcgagc ggctggttca
     2281 tttctgggcg aaccatttcg cggtatccac cgaaaaaccc ggcgtggcgg cactggccgg
     2341 ctcattcgaa gcggaagcga tccgcccgca cgtactcggc cgtttcgagg acatgctggt
     2401 cgcggtggaa cgtcatccgg ccatgcagct gttccttgac cagacgcgct cggtcggacc
     2461 agacagcatg gcggccatgc gtgccgcaca gcgcaatcca gacatcaagc gtggtctgaa
     2521 cgagaatctg gcgcgcgaaa tcatggagtt gcacaccctt ggcgtgagga gcggctataa
     2581 ccaggacgac gtcaccgaat tcgcccgcgc gctgaccggc tggagcctcg ccggcaatcc
     2641 gggcgctgcg ggcaatcctc gcaacacggg cgggcaaacc atgccgccga acgccgcccc
     2701 gggcaccttc gtgtttcgcg ccgcactgca cgagccgggc gcgagaaccg tcatgggccg
     2761 ccgctacgat cagccgggag aagagcaggc gttggcgatc ctgcacgacc tcgccagtgc
     2821 gcccgcgacg gcgcagcaca tcggcggcaa gctcgcccgt cacttcgtcg cggacaatcc
     2881 gccgccggac gtgagcgggc ggctggcgag cgcgttcgcg cgcagcggcg gcgatctgcc
     2941 gaccgtgtac cggacgctga tcgacatgcg agaggcgtgg tcgccggtcg ccgtgaagtt
     3001 caagacgccg tgggaatggg ccatttcttc gatgcgcggg ctcggctggc aggacctcgg
     3061 caacctgcaa gccgctccga tcctgacgca actcggtcag ccggtatggc gtccgggctc
     3121 gcccgccggt tacgacgaca ttgccgcgag ctgggcggcg ccggacgcgc tcgtgcgccg
     3181 ggtcgaagtg gcgcagcgct tcgcctcgcg ggtcggcgac cggctcgacg ctcgctcgct
     3241 cgggcccacc ttgctggccg gctcgctgag tgcaccgact gccacggcgg tgtcgcgggc
     3301 cgaaagcgcg tcgacggcga ttgcgctgct gctggtctca ccggattttc aacggagatg
     3361 aatgccatgc tttcacgccg caagtttctg agcgtcgccg ccgcgggcgc gggcgctatc
     3421 ctcgtgtcgc cgcggatcgc ttttgccagc gtggccaccg atcgccgctt cgtgttcgtg
     3481 atccagcgcg gcgcggccga cggcctgaac atcgtcgtgc cgtatgccga gccggcttac
     3541 gcgacactgc gcggtgcgct cgccatcgat gcatccaacg caacccggct tgacggcacc
     3601 ttcgcgctgc atccctcgct cgcgcggacc gccgcgatgt atgcggacca tcaggcgctg
     3661 ttcgtgcacg cggtggcctc gccgtatcgt gaccgctcgc atttcgacgg gcagaacgtg
     3721 ctggagaccg gcggcacgtc gccatatcag atgaaggacg gctggctgaa ccgcctcgtc
     3781 gcgtcgatgc cggccacgcg cgagaacgcg atcgctttcg cgccgaccgt gccgatggcg
     3841 ctgcgcggca aggcggcggt gacgtcgtat gcgccctcgg gcttgccgca ggcgcctgac
     3901 gatctgctca tgcgggtctc gcaactgtac gaccaggatg cgcaattgcg gccgctatgg
     3961 cagtcggcga tgaccgcgcg cgggctcgcg ggtgacgccg gcgcgcgtca ggacccggcg
     4021 agcctcggca aactcgcggc tggttttctg tcgcgcgacg acggcccgcg tatcgcgatg
     4081 atcgaaaccg ctggctggga cacgcatacc gcgcagaacg cgcgccttgc caatcagctg
     4141 aaagcgctcg atacgatgct cgcggcattg cgcgacggca tgggtccgtt atggcgcaag
     4201 accacggtgg tggtggcaac cgagttcggc agaaccgccg ccgccaacgg cacgggcggc
     4261 accgatcatg gcaccggctc ggtggcgatg gtactgggcg gctcggtggc gggcggacgc
     4321 gtgcttaccg actggccggg cctgaaaccg ggcgagcttt acgaagcgcg cgacctcaag
     4381 ccgacgatgt cgctggatgc gctgatcgcg ggcgccgcga gcgaaagcct cggactcgat
     4441 ccgcagcgca cggccgtcac gctgttcggc caaaccgcgg gcacgcagcc gatgaccggg
     4501 ctcgtgcgcg catagcggtg ggggcaaaac acaaggcact agcatcccgg cgagcggatt
     4561 ggaccgacgt gcgccgggta accggtacgg taggactctg aaacgatgac gctacctcgt
     4621 cggcctggtc cgatggggac ggttccaaat accaggacaa catcaggatg ataggcatcg
     4681 ccgcagattg gcgcttgcgg cgatgcctat cgcgacctgt tcgtggctta tcaatagcca
     4741 ggcgagatca acccccgcca ccgccttgcg taccgagcgt cagcttgttg ttcaccgact
     4801 tgacgccggc aacgttcttt gccacttcct ccgcttgcgg gatctgcgcg ccatcgggca
     4861 ccgagccagt caaggtcacc gcgccgccac gcgcccgaac aaacacgttc gacacatcaa
     4921 aaccctgcgt tttcgacaaa gccttgcgca cgctcagccc taacttacgg tccgcactct
     4981 tcatcgcctt cggattcgga ctcgcacttg cggccggcgc cgccatcgcg tcgctggact
     5041 gggcataggc actggacaca gtcgctacac acaacgcgat gccaagcgcc ttcaatagat
     5101 tcactgtttt cacgttttct ccatcctcga aaagttgagt tcgcatcgtt gcatgcaaaa
     5161 aaccacgcgc ctgcgagcgt tttgctgccc gtgtggagtt ccctgtcttg tagaagagtg
     5221 acgttgtgaa gctcccagcg cgcttaaaca atactccagc aaacgcacta gtgttgaaaa
     5281 aactgacctg agcgcggtgt tgctgggcgc cggtggcggg atttgcctag catggcatgt
     5341 tcgatcattg aggggggcag ggcggacagt tgcctgcgtt gcagtcggtt gtttgcatgt
     5401 cttgcggggt tgggattttc ttgatttggc tctttggcct ttccttgaat tgatattggt
     5461 ctgtttgcct gcggcggtgg gtttggttgt ttgccttcgg cgttggcctt tccttgaatt
     5521 gatattggtc tattagcgtt gcccctgtgc ggggcaggca cctacttttc tttgcctgcc
     5581 gcaaagaaaa gtaggcaaaa gaaagcggct caccccgcta gctcttaagc ggggcccccg
     5641 cacagttacg gtagtggtgc atctggaatc tgtgttctcg cacattccgc ggtggtgaca
     5701 aagggctcat gcttcccgcc tcgcactgcg tgctcgccgg aagggttcat cggaaaaaat
     5761 gggcttcgtt tggcgccgtg ggggccttcg gcttcgcctc ggcgaggggt tcacgtaacg
     5821 taatgacgaa cccgcaagcg agtgggcgtg gcaagcacgg accagacgaa acgggccgtc
     5881 gcggctgcgg agacggcaat cgcgctaacg gtag
//
LOCUS       DATASH010001104         4373 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4545297,
            whole genome shotgun sequence.
ACCESSION   DATASH010001104 DATASH010000000
VERSION     DATASH010001104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4373
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4545297"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..1497
                     /gene="boxB"
                     /locus_tag="VHV99_29285"
     CDS             70..1497
                     /gene="boxB"
                     /locus_tag="VHV99_29285"
                     /EC_number="1.14.13.208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HEX3639504.1"
                     /translation="MSTINYSEKIPNNVNLADDRALQRALEQWQPNFLSWWGDMGPEG
                     SHGYDVYLRTAVSVDAGGWAHFDHVKMPDYRWGIFLTPGEQDRKIHFGEHKGQAAWQD
                     VPGEHRANLRRIIVTQGDTEPASVEQQRHLGLTAPSMYDLRNLFQVNVEEGRHLWAMV
                     YLLHRYFGRDGREEAEALLGRRSGDDDNPRILGAFNEKTPDWLAFYMFTYFTDRDGKF
                     QLSALAESGFDPLARTTKFMLTEEAHHMFVGESGVSRVIQRTAQVMNELGTDDVAKIR
                     AAGVIDLPTIQRYLNFHYAVTIDLFGADHSSNAATFYSSGLKGRYEENKRDDDHQLNG
                     QSYKLLDVQDGKLVEREVPMLNAMNEVLRDDYIKDSVAGVGRWNKVLEKAGIDFRMTV
                     PHKAFNRQIGTFAGTRVSPDGRVVSETEWAANEAKWLATPEDRAYVASLMGRVTEPGK
                     FANWIAPPAMGVNRQPVDFEYVRFN"
     gene            1563..2807
                     /gene="boxA"
                     /locus_tag="VHV99_29290"
     CDS             1563..2807
                     /gene="boxA"
                     /locus_tag="VHV99_29290"
                     /EC_number="1.14.13.208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxA"
                     /protein_id="HEX3639505.1"
                     /translation="MNGPVSIEVLRQHLIDPEICIRCNTCEETCPVDAITHDENNYVV
                     KADVCNGCMACVPPCPTGAIDNWRTVLKADAYPIDEQFKWDVLPEQNTMAVPAAEAAA
                     AAAGTSGDTSADASGIEVDTVRGSVVPPWSAAKPYVNLYTHKAPTTATVVGNYRLTDV
                     STDSDIHHIVLDFGSMPFPVLEGQSIGILPPGPTADGRTHHARQYSIASPRDGERPGY
                     NNASLTVKRVSQQYGDALDGVCSNYLCDLKKGDVVNVIGPFGGTFLMPNHPNSHLLMI
                     CTGTGSAPMRAMTEYRRRRRLKGATGKLMLFFGARTKEELPYFGPLTNLPKDFIDTNL
                     AFSRTPGQPKRYVQDAMRERAVDVAHMLKDDNTHIYVCGLKGMEDGVLQALREIGEQH
                     QLDWDALWMKLKREGRLHLETY"
     gene            complement(2891..3655)
                     /locus_tag="VHV99_29295"
     CDS             complement(2891..3655)
                     /locus_tag="VHV99_29295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006052527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX3639506.1"
                     /translation="MKRDAAHMPMKANMRDDNVAADITENDQTRDETRTPLRRRKAHI
                     RESNEAHLLACAEAVFAERGLDGTSTAMIAERAGLPKANLHYYFPTKLALYRRVLEDL
                     FEDWYRAADTFECSDDPVEAIGGYVRAKMELSRRRPLGSKVWASEIIHGAEHMEDILT
                     GRVKPWLDSRVKVIDDWIARGLLAPVNAQTLMYMIWATTQHYADFDAQIRALRGKRAL
                     TQKAFEATTEEVVQLVIRACGAVSPAQHEDGADPSR"
     gene            3958..>4373
                     /locus_tag="VHV99_29300"
     CDS             3958..>4373
                     /locus_tag="VHV99_29300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065405.1"
                     /note="allantoate amidohydrolase and N-carbamoyl-L-amino
                     acid amidohydrolase are very similar; the allantoate
                     amidohydrolase from Escherichia coli forms a dimer and
                     binds zinc ions for catalytic activity and catalyzes the
                     conversion of allantoate to (S)-ureidoglycolate and
                     ammonia; carbamoyl amidohydrolase from Bacillus sp.
                     converts N-carbamoyl amino acids to amino acids, ammonia,
                     and carbon dioxide; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HEX3639507.1"
                     /translation="MNAVSEALRHAEPATSIKVDGKRLWDSLMTMAKIGATPKGGVCR
                     LALTDLDKEGRDLIVSWAKEAGCTVSVDQMGNVFMRRAGRNPDALPVMTGSHADSQPT
                     GGRFDGIYGVLGGLEVIRSLNDHGIETEHPVEVVIWT"
BASE COUNT          887 a         1289 c         1353 g          844 t
ORIGIN      
        1 tccttgcccg ctacgcgcct ctcacactta ggcaccgcaa agcaccgcat tccgatcagg
       61 ataccgacca tgtcaacgat caactacagc gaaaagattc cgaacaacgt caatctcgcc
      121 gacgatcgcg ccttgcaacg cgcgctcgaa caatggcagc cgaattttct gtcgtggtgg
      181 ggcgatatgg gccccgaagg ctcgcatggc tatgacgtgt atctgcgcac ggcggtgagc
      241 gtcgacgcgg gcggctgggc gcatttcgat cacgtgaaga tgccggacta tcgctggggc
      301 attttcctca cgcctggcga gcaggaccgc aagatccatt tcggcgaaca caagggtcag
      361 gctgcgtggc aggacgtgcc cggcgagcat cgcgcgaatc tgcgccgcat tatcgtgacg
      421 caaggcgata cggagccggc ttcggtcgag cagcaacggc atcttggcct gacggcgcct
      481 tcgatgtacg acctgcgcaa cctgttccag gtgaacgtgg aagaaggccg ccatctgtgg
      541 gcgatggtgt acctgctgca tcgctacttc ggccgcgatg gacgtgagga agccgaagca
      601 ctgctcggcc gccgttcggg cgacgacgac aatccgcgca ttctcggcgc cttcaacgag
      661 aaaacgccgg actggctcgc gttctacatg ttcacctact tcaccgaccg cgacggtaag
      721 ttccagttgt cggcgctcgc cgagtcgggc ttcgatccgc ttgcgcgtac cacgaagttc
      781 atgctgaccg aagaagcgca tcatatgttc gtgggcgaat ctggcgtgtc gcgcgtgatc
      841 cagcgtaccg cgcaagtgat gaacgaactg ggcacggacg acgtcgcgaa gattcgcgcg
      901 gccggcgtga tcgatttgcc gaccatccag cgttatctga acttccatta cgcggtgacg
      961 atcgacctct tcggcgccga tcattcctcg aatgcggcca cgttctacag ctccggcctg
     1021 aaggggcgtt atgaagagaa caagcgcgac gacgatcatc aactgaacgg tcaaagctac
     1081 aagctgctcg acgtgcagga cggcaaactg gtcgagcgtg aagtgccgat gcttaacgcc
     1141 atgaatgaag tcctgcgcga cgactacatt aaagattcgg tggcgggcgt cggccgctgg
     1201 aacaaggtgc ttgaaaaggc cggcatcgat ttccgcatga cggtgccgca taaagcgttc
     1261 aatcggcaaa ttggcacgtt tgcgggtacc cgtgtgtcgc cggacgggcg cgtggtcagc
     1321 gagaccgaat gggccgcgaa tgaagcgaag tggctcgcca cgccggaaga ccgcgcctat
     1381 gtcgcttcgc tgatgggccg cgtcaccgaa cccggcaagt ttgcgaactg gattgcgccg
     1441 ccggcaatgg gcgtgaatcg tcaaccggtc gacttcgaat acgtgcgctt caactgagta
     1501 cggcgattga aaacatgtgg cgtgcggcat cgtatccgca cgaatatagg gtggaggaag
     1561 tcatgaacgg cccagtatcg atcgaagttc tcaggcagca cctgatcgat ccggaaatct
     1621 gcattcgctg caatacctgc gaagagactt gcccggtcga tgcgatcacg cacgacgaga
     1681 acaattacgt ggtcaaggcg gatgtctgca acggctgcat ggcttgcgtg ccgccgtgtc
     1741 cgaccggcgc gatcgataac tggcgcacgg tgttgaaggc tgacgcgtat cccatcgacg
     1801 agcagttcaa atgggacgtg ctgccggagc agaacacgat ggcggtgcct gcggcggaag
     1861 ctgccgcagc agcggcgggg acgtcgggcg atacatcggc ggacgcaagt ggtattgaag
     1921 tcgataccgt gcgcgggtcg gttgtgccgc cgtggtccgc tgcgaaaccg tacgtgaatc
     1981 tctatacgca caaggcgccg actaccgcga cggtggtggg caactaccgg cttaccgacg
     2041 tctcgacgga tagcgacatc catcacatcg tgctcgattt cggttcgatg ccttttcctg
     2101 tgctggaggg ccagtcgatc ggcatcctgc cgccggggcc gactgccgac ggtcgcacgc
     2161 atcatgcgcg gcagtattcg atagcgagcc cgcgcgatgg tgaacggccc ggctataaca
     2221 acgcttctct caccgtgaaa cgcgtttccc agcagtatgg cgacgcgctg gatggagtct
     2281 gctcgaatta tctgtgcgat ctgaagaagg gggacgtggt caacgtgata gggccgttcg
     2341 gcggcacatt cctgatgccg aatcatccga actcacatct gctgatgatc tgcaccggca
     2401 ccggttcggc gccaatgcgc gcgatgaccg agtatcgcag gcggcgtcgt ctgaaaggcg
     2461 cgaccggcaa gctgatgctg ttcttcggcg cgcgcacgaa agaagagttg ccgtacttcg
     2521 ggccgctcac gaatctgccg aaagatttca tcgatacgaa tctggcattt tcgcgcacac
     2581 cgggtcagcc caagcgctac gtgcaggacg cgatgcgcga gcgcgcggtc gacgtcgcgc
     2641 atatgctgaa ggacgacaac acgcatattt acgtgtgcgg gttgaagggg atggaagacg
     2701 gtgtgctgca ggcgcttagg gaaatcggcg aacagcatca gctcgattgg gatgcgctgt
     2761 ggatgaagct gaagcgggaa gggcggttgc atctggagac gtattgaatc accaggcatc
     2821 gagagagagg catcacgcaa tagtgcgaag gcccggtcgc ggttaaaaag cgcagccggg
     2881 tttttgcttt ttacctcgac gggtccgcgc cgtcctcgtg ctgtgccggc gacactgccc
     2941 cacacgcccg aatcaccaac tgcacgactt cctcagtcgt tgcttcgaac gccttctgcg
     3001 tcaacgcacg tttgcctcta agcgcgcgaa tctgcgcgtc gaagtcggcg taatgctggg
     3061 tggttgccca gatcatgtac atcaacgttt gcgcattaac gggcgctaac aacccgcgtg
     3121 cgatccagtc gtcgatcacc ttcacccggc tatccagcca cggcttcacg cgccccgtga
     3181 gaatgtcctc catatgctca gcgccgtgaa tgatctcgct tgcccacact ttcgagccta
     3241 atggccggcg gcgcgacaat tccattttcg cgcgtacgta gccgccaatc gcctcgaccg
     3301 gatcgtcgct gcattcgaac gtatccgctg cgcggtacca gtcttcgaac aggtcttcca
     3361 gcacgcgccg gtacaacgcg agcttggtcg gaaagtagta atgcaggtta gctttcggta
     3421 agcctgcacg ctccgcgatc atcgccgtac tggttccgtc gagtcctcgc tccgcgaaca
     3481 ccgcttcggc acacgccaat aaatgcgctt cattcgactc gcgaatgtgc gccttgcgcc
     3541 gccgcaaagg cgtgcgggtt tcgtccctgg tctggtcgtt ttccgtgatg tcggcagcca
     3601 cgttgtcgtc tctcatgttg gccttcatcg gcatgtgtgc cgcgtcgcgc ttcattctag
     3661 ccgttcgggc gcggatcaac acgcgcattc gaagccgttg aaaaacgcgt gctgcagtgc
     3721 aatggcacgc ttctcgctat gtatcccgcc gtacgaaaag cattgatttg gcgggcttaa
     3781 tcgtctacaa cctgtccaac tggacaggat tgagagagcg ggcgatgccc cagggtttgc
     3841 cctctgatgc agaggcgatc gaaggcatcg aatgtgcacc gccgccgtgc ggccacgccc
     3901 caaaacccgc cgcccacgca ccagttccat gtcggttcat cgaaaggagc gagacgaatg
     3961 aatgcggtat ccgaagcact gaggcacgcg gaacccgcca cgtcgatcaa ggtcgacggc
     4021 aagcggctct gggacagcct gatgacgatg gcgaagatcg gcgcgacgcc caaaggcggc
     4081 gtctgccggc tggcgctgac cgacctcgac aaagaggggc gcgacctgat cgtcagttgg
     4141 gcgaaggaag cgggttgtac ggtcagtgtc gatcagatgg gcaatgtatt catgcgccgc
     4201 gccggacgca atcccgatgc gttgccggtc atgaccggtt cgcacgcgga ctcgcagccg
     4261 accggcggcc gctttgacgg catctacggc gtgctcggcg gactcgaagt gatccgcagt
     4321 ctgaacgatc acggcatcga gaccgaacat ccggttgagg tggtgatctg gac
//
LOCUS       DATASH010001105         4355 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6519278,
            whole genome shotgun sequence.
ACCESSION   DATASH010001105 DATASH010000000
VERSION     DATASH010001105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4355
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6519278"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(322..1881)
                     /locus_tag="VHV99_29305"
     CDS             complement(322..1881)
                     /locus_tag="VHV99_29305"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX3639508.1"
                     /translation="MPLPPLLSLLIWIPIVAGLVVLRAGSDAARSRTRWLALIGAVAG
                     FLPVIPLVSNFRNDVTSMQFVENFRWSPTFDIAWHVGVDGISLWLVALTALTTIIIVV
                     ASWESITERTAQYFGAFLMLSGFMQGVFTSMDGMLFFISFEATLIPLYLLIGTWGNSN
                     RAYAAVKFFFVSFLGSLLMLMSMLYLYSQTHSFDLALWRETHLGTWPQVLIFLGFLAA
                     FGVKVPMWPLHTWLPDVHLDGPTGAAVMIGMLKLGGYGLLRFALPIAPQASHFFAPMM
                     ITLSLIAVIYSSLLALVQTDMRRLLAYSTIAHMGLVTLGLFVFNRIGQAGAIVQMLSY
                     GVVSGAMLLCTGMLYDRTKTASIAEYGGVANTMPRFATFVMLFSMANIGLPGTSGFVG
                     EFMVLMGTIRFNFWIGAIAASTLVLSAAYTLWMYKRVIFGAIANARVAKLQDLGKREF
                     ALLGVMALLVLAIGIHPKPFTDAIDPSVGTLLAQSERSEHPSDSVPADGGEHLQAAVP
                     AAATVAARTPG"
     gene            2571..3320
                     /locus_tag="VHV99_29310"
     CDS             2571..3320
                     /locus_tag="VHV99_29310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3639509.1"
                     /translation="MVLRDEGVVPALECRGLSKTYGAGRIVLAHLDFILAPGEFVAIM
                     GDSGVGKSTLLNLVAGLDSADSGDVIIDGSPVSRLDDNAATRLRRQKLGFVFQAFHVL
                     PHLTLAQNVALPLLLNDLPTAGALDMLAAVGLSGRGDDFPRQLSGGELQRVAIARALV
                     HRPKLILADEPTGNLDPDTAHDVLGLFRAQSKATGAATILVTHSEAAAAVADRILILS
                     DGGLHASSGRSAFSDSHPDARSTARSADDAR"
     gene            3310..>4355
                     /locus_tag="VHV99_29315"
     CDS             3310..>4355
                     /locus_tag="VHV99_29315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX3639510.1"
                     /translation="MHADPRPLQRMRGHRMLARWLLGAEWRSHRGRALVAIATIALGV
                     ALGYAVQLINSAAFNEFSAAARSLSGQADLQVRAAQPRFDESIYPRLSTEPGVALASP
                     VLALDVTVPDQNAALPVLGIDVFKASRIAPDLIGVPSPERPFDTLAADTVFLSAAAQQ
                     WLKVKIGDDVALQSGTSIVHLRVAGGLVRTRPGQRLAVMDIAAAQWKFGKVGKLSRID
                     VQLERGIDRERFRRDLQARLGGQWVIAETRDAESRTDRLSRAYRINMNVLALVALFTG
                     AFLVFSTQALGVVRRRAQFAMLRVLGLTRGQLLGQILMEGALLGLLGSLSGLVLGYAM
                     ASGALRFFGSDLGGG"
BASE COUNT          921 a         1314 c         1325 g          795 t
ORIGIN      
        1 ccgttaataa gcgctcgcat aataagggcg cgtaaagtgg atataaacac tcattttcgc
       61 aacgatctat tgcggcaaaa caccgtgtga catgttgccg cacagcgatg cgcgcgggga
      121 tctgccatgc cgttccgcaa actagcggcg gcgctgccgg atcacaaaag tccccttcat
      181 caagcgttaa aacgcggcgc cacctgaatg attgagccgc gctacatgct cgataagaga
      241 cgtgtgtcga tgcgaaaaga acgcgccggc aaaagcagca tccttacaac gaatgcggca
      301 gcggctcagc gcttcgttcc atcagcccgg cgtgcgtgcc gcgacggttg cggctgcagg
      361 taccgccgcc tgcagatgct cgccaccgtc agccggcacg gagtccgaag gatgctcgct
      421 tcgttccgat tgggccagca gcgtaccgac cgaggggtcg attgcatcgg tgaacggctt
      481 cggatggata ccgatagcca gcaccagcaa agccatcacg ccgagcaacg cgaactcgcg
      541 cttgccgagg tcctgcagct tcgcgacgcg tgcattggca atcgcgccga aaatcacgcg
      601 cttgtacatc cacagcgtgt aggccgcgct gagcaccaac gtggacgccg caattgcacc
      661 gatccagaaa ttgaagcgga tcgtacccat caacaccatg aactcgccga cgaaacccga
      721 ggtgcccggc agaccgatat tggccatcga gaacagcatt acgaatgtgg cgaaacgcgg
      781 catggtgttg gcaacgccgc cgtactcggc gatcgaagcg gtcttcgtgc ggtcgtaaag
      841 catgcccgtg cacagcagca tggcaccgga aaccacgccg tacgacagca tctgcacgat
      901 cgcgcccgcc tggccgatac ggttgaagac gaacaaaccg agcgtcacga gacccatgtg
      961 cgcgatcgtc gaataggcga gcaagcggcg catgtcggtc tgcaccagtg ccagcaggct
     1021 cgaatagatc accgcgatga gcgagagcgt gatcatcatc ggcgcgaaga aatggctggc
     1081 ctgcggtgcg atcggcagcg cgaaacgcag cagaccgtag ccgcccagct tgagcatgcc
     1141 gatcatcacg gcggcgccgg tcgggccgtc caggtggacg tcgggcagcc acgtatgcag
     1201 cggccacatc ggtaccttca cgccgaacgc tgcaaggaag ccgaggaaga tcaagacctg
     1261 cggccaggtg ccgaggtgcg tttcacgcca cagtgcgagg tcgaagctat gcgtctggct
     1321 gtacagatac agcatcgaca tcagcatcag cagcgaaccg aggaacgaga cgaagaagaa
     1381 cttcaccgcg gcatacgcgc ggttcgaatt gccccatgta ccgatcagca ggtacagcgg
     1441 gatcaacgtc gcttcgaacg agatgaagaa caacatgccg tccatcgacg tgaacacgcc
     1501 ctgcatgaaa cccgagagca tcaggaaggc gccgaagtac tgtgcggtgc gctcagtgat
     1561 cgactcccac gaggcaacga cgatgatgat cgtggtcaat gcggtcaacg cgacgagcca
     1621 cagcgaaatc ccatccacac ccacgtgcca cgcaatatcg aacgtcggcg accagcggaa
     1681 gttctcgacg aactgcatcg acgtcacatc gttgcggaaa ttcgacacca gcggaatcac
     1741 cggcaggaaa cccgctaccg cgccgatcag cgcgagccat cgcgtgcgac tacgtgccgc
     1801 gtcagatccg gcacgcaaaa ccaccaggcc ggcgacaata gggatccaga tgagtaggct
     1861 aagaagcgga ggcaatggca tgtgttctgt tgaaaactag aaatacaaaa cgccgcatca
     1921 acgcccccga aaacgaaagc attgaaatga atctgtaggc tggatttcac tgaaatcgac
     1981 gtcgaagcag tgctgtctgc ctcgcgtata cccgcagtcg aatgatcaaa gggtgggaga
     2041 ttaccaagat aagaataatt ttgcagcgca ccaacgctac aacgcgccag gaagtgcctt
     2101 gttgccacgc ttgcccttgc gaaacatcga aaagtgtgca gacgcacaaa gccatttaag
     2161 cagggtatac accatgcttt ttgcatgtag aagaaagaag atagcggccg cttagcccct
     2221 cttgcacaaa cgcttctacg cgacgatcag attggaatgg atcatgctgg tgcataacgt
     2281 gccgagaggg ccgatttcga gcccccgcaa aaaatttagc gcgtccgcgc acagaaaatt
     2341 tttttgatcc ggcactctag agaaacgtcc gcgcgcggtc tgacactcac tcacttgact
     2401 gcctggcagc ccgctttcag atgggtttca gacgatgacg gcatgcacct gcgaggctta
     2461 cagaagccga atatacttcg gcggttcaca ggcccgacgc tcatacacgg tgcgctgttt
     2521 cttcgcacag ttggacaccg ccctcaccgg cctgacttat cggatatcgc atggtgcttc
     2581 gtgatgaagg tgtcgtcccc gcgctcgagt gccgcgggct tagcaagacc tacggcgccg
     2641 gccgcatcgt gctggcgcac ctcgatttca tactcgcgcc gggtgaattc gtcgcgatca
     2701 tgggtgactc cggcgtcggc aaatcgacgc tgctgaatct cgttgcaggg ctcgacagcg
     2761 cggatagcgg cgatgtgatc atcgacggca gtccggtctc gcgtctggat gacaacgcgg
     2821 cgacgcgttt gcgccggcaa aaactgggct tcgtgttcca ggcgtttcac gtcctgcccc
     2881 atctgacact tgcgcagaat gtcgcgctgc cgctgctgct caacgatctg cccacagccg
     2941 gcgcactcga catgctcgcc gccgtcggct tgagcggccg aggcgatgat tttccgcggc
     3001 agttgtcggg cggcgagttg caacgcgtcg ccattgcgcg agcgcttgtg catcgcccga
     3061 aactgattct cgccgacgaa cccaccggca atctcgatcc cgataccgca cacgacgtgc
     3121 tcggcctgtt ccgcgcgcag agcaaggcaa cgggtgcggc cacgatcctg gtcacgcact
     3181 cggaagccgc tgcggcggtg gcggatcgaa tcctgattct gagcgacggc ggcctgcacg
     3241 catcgtccgg acgcagcgcc ttttcggact cgcaccccga cgctcgatcc actgcgcgtt
     3301 cagccgatga tgcacgctga cccacggccg ttgcagcgaa tgcgaggtca tcgcatgctc
     3361 gcgcgttggc tgttgggcgc agagtggcgt agtcatcgcg gacgcgcact ggttgcaatt
     3421 gcaacgatcg cgctcggcgt cgcgcttggc tacgccgttc aattgatcaa cagcgcggct
     3481 ttcaatgaat tctcagcggc ggcgcgcagc ctgtcgggcc aagccgatct gcaggtgcgt
     3541 gcggcacagc cccgattcga cgagtccatc tatccgcgtt tgtctactga gcctggtgtg
     3601 gcgctggcga gtcctgtact cgcgctcgat gtgactgtgc cggatcagaa cgcggcgctg
     3661 ccggtgcttg gcatcgacgt cttcaaggcg agccgcattg cgcccgatct gatcggcgtg
     3721 ccatcgccgg agcggccgtt cgatacgctc gcggctgata cggtgtttct ctcggcggcg
     3781 gcacaacaat ggttgaaggt gaagatcggc gatgacgtgg cgctgcaaag cggcacgtcg
     3841 atcgtgcact tgcgcgtggc cggtggcctc gtaaggacgc ggccgggtca gcggctcgcg
     3901 gtgatggata ttgccgccgc tcagtggaag ttcggcaagg tgggcaagct gtcacgtatc
     3961 gacgtgcaac tcgaacgcgg catcgatcgc gaacgcttca ggcgcgacct gcaagcacgg
     4021 ctcggcggcc agtgggtgat cgccgagaca cgcgatgctg aaagccgcac cgatcgtctg
     4081 tcgcgcgcct atcggatcaa tatgaatgtg ctcgcgctcg tggcactttt caccggcgcc
     4141 ttcctcgtgt tttcgacgca ggcgctgggt gtggtgcggc gccgtgcaca gttcgcgatg
     4201 ctgcgtgtgc ttggactgac gcgtggccag ttgttaggtc agatcctgat ggaaggcgcg
     4261 ttgctcggcc tgctcggttc gctgtccgga ctcgtgcttg gctatgccat ggcgagcggt
     4321 gcattgcgct ttttcggcag cgacctgggc ggtgg
//
LOCUS       DATASH010001106         1515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6720682,
            whole genome shotgun sequence.
ACCESSION   DATASH010001106 DATASH010000000
VERSION     DATASH010001106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6720682"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..89
                     /locus_tag="VHV99_29320"
     CDS             <1..89
                     /locus_tag="VHV99_29320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004522525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine ABC transporter permease"
                     /protein_id="HEX3639511.1"
                     /translation="YLVITTISNFVLMYLEKRYSTGVRKADL"
     gene            86..799
                     /locus_tag="VHV99_29325"
     CDS             86..799
                     /locus_tag="VHV99_29325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3639512.1"
                     /translation="MIEIIQEYWRNYLYTDGYRFTGLAITLWLLVVSIGLGFCLSIPL
                     AVARVSKKKWLGRLVWLYTYIFRGTPLYVQLLLCYTGLYSLEVIRNHELTNAFFRDGM
                     HCTLLAFTLNTCAYTTEIFAGAIKATPYGEIEAARAYGMSSFTLYRRVILPSALRRAL
                     PYYSNEVILMLHATTVAFTATVPDILKIARDVNSATYQSFNAFGLAALLYLLISFALV
                     WLFRRAERRWLAYLRPQGK"
     gene            850..>1515
                     /locus_tag="VHV99_29330"
     CDS             850..>1515
                     /locus_tag="VHV99_29330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014896370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3639513.1"
                     /translation="MTTKKQKLFVDELHKQYGDNEVLKGVSLKANAGDVISVIGSSGS
                     GKSTMLRCINFLEQPNAGRIFVDGEEVRTQIGKAGALRVSDAKQLQRVRTKLSMVFQH
                     FNLWSHMNVLENIIEAPVNVLSLKRKEAEERAREYLEKVGLAPRLEKQYPSHLSGGQQ
                     QRVAIARALAMHPDVMLFDEPTSALDPELVGEVLKVMQTLAEEGRTMIVVTHEMAFAR
                     NVSN"
BASE COUNT          292 a          479 c          442 g          302 t
ORIGIN      
        1 tctatctcgt catcaccaca atctccaact tcgtgctgat gtacctcgaa aagcgttact
       61 cgaccggcgt gcgaaaggcg gatctatgat cgagatcatt caggaatact ggcgcaacta
      121 tctttatacc gacggctacc gtttcaccgg cctcgcgatc acgctgtggc tgctggtggt
      181 gtcgatcgga ctgggcttct gcctgtcgat tccgctcgcg gtcgctcgcg tgtcgaagaa
      241 gaaatggctc ggccgtctgg tgtggctgta tacgtatatc ttccgcggca cgccgctcta
      301 cgtgcaactg ctgctctgct acaccggctt gtacagcctc gaggtcatcc gcaatcacga
      361 gctgaccaac gcgttcttcc gcgacggcat gcattgcacg ctgctcgcgt tcacgctcaa
      421 cacctgcgca tacaccaccg agattttcgc cggcgcgatc aaggccacgc cgtatggcga
      481 gatcgaggcg gcgcgcgcct atggcatgtc gtcgttcaca ctgtaccgcc gggtgattct
      541 gccgtcggca ctgcgccgcg cgttgccgta ctacagcaac gaagtgatcc tgatgctgca
      601 cgccaccacc gtcgcgttca ccgcgaccgt gccggacatc ctcaagatcg cccgcgacgt
      661 gaactccgcc acgtatcagt cgttcaatgc gttcggcctc gccgccttgc tctatttgtt
      721 gatttcgttt gcgctcgtgt ggctgttccg ccgcgccgag cgtcgctggc tcgcttacct
      781 gcggccgcaa ggcaagtaac caagggcacc gactcaagcc ccaagcacgt aagcccgcca
      841 aggatcctga tgaccaccaa gaagcaaaag ctcttcgtcg acgagcttca taaacagtat
      901 ggcgacaatg aagtcctcaa gggcgtgtcg ctgaaggcca acgccggcga cgtgatcagc
      961 gtgatcggct cgtccggttc gggcaagagc acgatgcttc gctgcatcaa ctttctcgag
     1021 cagccgaatg ccgggcgcat cttcgtcgac ggcgaggaag tccgcacgca gatcggcaag
     1081 gccggcgcct tgcgcgtatc ggatgcgaag cagttgcagc gcgtgcgcac caagctgtcg
     1141 atggtgttcc agcacttcaa cctgtggtcg cacatgaacg tgctcgagaa catcatcgag
     1201 gcgccggtca acgtgctgag tctgaagcgc aaggaggccg aagagcgtgc gcgcgaatat
     1261 ctggagaagg tcggcctcgc tccgcgtctg gagaaacagt atccgtcgca tctgtcgggc
     1321 ggccagcagc agcgtgtggc gatcgcgcgc gcgctcgcga tgcatcccga cgtgatgttg
     1381 ttcgacgagc cgacgtccgc actcgacccc gaactggtcg gcgaagtcct caaggtcatg
     1441 cagacgctcg ccgaggaagg ccgcacgatg atcgtcgtca cgcacgaaat ggcgttcgcg
     1501 cgcaacgtat cgaat
//
LOCUS       DATASH010001107        26606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2961021,
            whole genome shotgun sequence.
ACCESSION   DATASH010001107 DATASH010000000
VERSION     DATASH010001107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 26606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26606
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2961021"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..1879
                     /gene="dnaG"
                     /locus_tag="VHV99_29335"
     CDS             14..1879
                     /gene="dnaG"
                     /locus_tag="VHV99_29335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEX3639514.1"
                     /translation="MIPPSFLQDLLNRVDIVDVVGRYVQLKKGGANFMGLCPFHNEKS
                     PSFTVSPTKQFYHCFGCGAHGTAIGFLMEHVGSTFPEAVNELAQSVGLTVPNEPSPGY
                     SGGSGGYSPAASKAVTTALSDVMQTACDFYRKQLRGAPVAIQYLKKRGLTGEIAARFG
                     LGYAPDGWQNLEPAFPNYRDDALVESGLVIVSEKSDAQGQNRRYDRFRERIMFPIRNV
                     KGQVIGFGGRVLDGGEPKYLNSPETPLFNKGSELYGLFEARLAIREQHYVLVVEGYMD
                     VVALAQLGFQNAVATLGTACTPIHVQKLMRQTDTVIFSFDGDSAGRRAARRALDACLP
                     HAADNRTIRFLFLPSEHDPDSYVREFGTEAFAEQVERAMPLSQFMLNEVLTGKELDQP
                     EGRARALFDAKPLLQQLPANALRAQIMHMFADRLDVPFDEVAALCEVDARIAAVARSA
                     PARKDRRSVTGIEEKTLRNLVMYPRTVAVLDEEAEQALLSVTRHGELFEEVTTHARAL
                     GDSAEFQLLSDLLRNGANAPTFEEIFRKILDYDENVRDLLLKDPEDATVIEERREQER
                     IVGEELKAAILKMRYDAYCDRLEQLSRQSRHTPEEFTELTSLNQKRAEMKRQLGL"
     gene            1973..4363
                     /gene="rpoD"
                     /locus_tag="VHV99_29340"
     CDS             1973..4363
                     /gene="rpoD"
                     /locus_tag="VHV99_29340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011658300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEX3639515.1"
                     /translation="MAKTPGGKKAPGTGSNEPTRKVTEAKLASSSRKTSATSVTPAAG
                     KKPSTTSAARAEPVRAAKAVPPPATKGPGVRSAREAPAAQDDAVERETPVSTVQPAVV
                     QQPRVETTAGTTNSMTKKLNETPVDDDATQSEETPAAVPGKVEKVKARDRRAKEKALL
                     KDAFASSQPGTVEELEERRSKLRALIKLGKERGFLTYAEINDHLPDNFTETEAIEGII
                     STFNDMGVAVYEQAPDAETLLLNDNAPAASSDDEVEEEAEVALSTVDSEFGRTTDPVR
                     MYMREMGTVELLTREGEIEIAKRIEDGLKHMVMAISACPTTIADILAMAERVANEEIR
                     IDELVDGLIDANAEDADGFSAQEAEAIESEDEEVEEEDADDEEEDDGTAQATANAAQM
                     EALKRASLEKFAMISEWFDKMRRAFEKEGYKSKSYLKAQETIQNELMTIRFTARTVER
                     LCDTLRAQVDEVRQVERQILHTVVDKCGMPRAEFIARFPGSETDLEWADKIVGEGHAY
                     SGILTRNIPAIREQQQRLLDLQARVVLPLKDLKETNRQMAAGELKARQAKREMTEANL
                     RLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRS
                     IADQARTIRIPVHMIETINKMNRISRQILQETGLEPDPATLAEKMEMPEDKIRKIMKI
                     AKEPISMETPIGDDDDSHLGDFIEDTNTVAPADAALHASMRDVVKDVLDSLTPREAKV
                     LRMRFGIEMSTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSDKLKSFLVGN"
     gene            4518..4605
                     /locus_tag="VHV99_29345"
     tRNA            4518..4605
                     /locus_tag="VHV99_29345"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4553..4555,aa:Ile,seq:cat)
     gene            5501..5671
                     /locus_tag="VHV99_29350"
     CDS             5501..5671
                     /locus_tag="VHV99_29350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639516.1"
                     /translation="MQTDPTIDPVADPANQPLDDPEAQPRPLPGTSPETDPPAPRNSL
                     ADDEALRWSRTS"
     gene            6620..7783
                     /locus_tag="VHV99_29355"
     CDS             6620..7783
                     /locus_tag="VHV99_29355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX3639517.1"
                     /translation="MKKSLIVVAVAASFASVAHAQSSVTLYGLLDAGLTYTSNVAPAN
                     GVGHGNAKWAAGSGGINQSMFGLRGSEDLGGGLKAIFTLESGFNVNNGKFANNNGMFN
                     RQAFVGLSSAQFGTVTLGRQYDAAQDYLAPLTATGSWGGTYFAHPFNNDNLNTNGGYA
                     VNNSIKYSSANYAGFTFGGTYGFSNQAGAFANNREYSLGAAYQWQGLHLGAAYAQQNN
                     PGATNAAGTASNGGASDGSILGLAGNFRQREFGAAASYSFGPATVGAAWTQSRIDNVV
                     GTQQSLSANNYEVNGKYNLTPALALGVAYTFTDGKGYGVAADGGSASVRYHQIGLQAD
                     YSLSRRTDVYAQAVYQHAMGDGGVASIYSGDNTQSPSSSKNQTAATVGLRHRF"
     gene            complement(7879..8202)
                     /locus_tag="VHV99_29360"
     CDS             complement(7879..8202)
                     /locus_tag="VHV99_29360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639518.1"
                     /translation="MSRALILTINHGPFAAVKWQTTRALTLTGKAYHDHMSNAAIAIR
                     SRRGLSMCCRKRTEVYGTVDFDKILGTAPVESPGKSNQTGVALGLRTIFQVTYVPQIH
                     QARDI"
     gene            complement(8203..9762)
                     /locus_tag="VHV99_29365"
     CDS             complement(8203..9762)
                     /locus_tag="VHV99_29365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX3639519.1"
                     /translation="MNRLTLKQKLWVPILLCWAALLVVTIVNAFDARNTQMDARRADL
                     ADVIDMAVSIVADYAKQADTGKLPVDEAKQQAIARINAQRYGTSGYVTIVRGDSVMVA
                     HPMSPNLNGKDMSGFRDAKGNALYHDIAQAGGSAAGAGYLRYWWPKPGETAPSEKIGY
                     VKRFVPWNWDFIAGAYMDDIQAQFYATLARSAGMLVLLGALASFVASRVVRNVSRSIG
                     GEPSVAAAIAMQIARGDLATHIDLRRDDTSSLLFSLREMRSQLAATVGRIKTSAETIT
                     VASKEIAAGNLDLSSRTEEQAASLQQTAATMDELTKTVTQNADSAATESELATDASAI
                     AARGGQVVNDVVEKMAGITDSSRKIGEIISVIEGIAFQTNIMALNAAVEAARAGEEGR
                     GFAVVAGEVRILAQRSATAAKEIKVLIDQSVAQVGEGSTLAGKAGSTIGEVVDAVRRV
                     TAIMNEISAASKEQSSGIGEVNLAIRQMDDVTQRNAALVEQAAAAAGSLDEQTERLRN
                     AVAAFKVEQHA"
     gene            10222..11853
                     /locus_tag="VHV99_29370"
     CDS             10222..11853
                     /locus_tag="VHV99_29370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEX3639520.1"
                     /translation="MKHMFEEGLERREANYVPLTPIDFIVRAAEVYGERLAVVHGEIR
                     RNWRETYERARRLASALRQAGIERGDTVAALLPNIPPMIEAHFGVPMAGAVLNTLNTR
                     LDVSSLLFMLRHGEAKALIVDTEYSELAHRAAQEFPELRVISVADAMPADADRFIRAT
                     DYEAFLQSGDPEFAWTLPADEWDAIALNYTSGTTGDPKGVVYHHRGAYLNALSNILEW
                     DMPRHAVYLWTLPLFHCNGWCFPWTVAARAGVNVCLRKFDAKTVFDLIRHVGITHYCG
                     APIVQSALANAPAEWREGITHRVSTMVAGAAPAPAVIARMKEIGFDLTHVYGLTETYG
                     PAAVCAKQEEWETLDDSARAEMNARQGVRYHLQAAVTVLDPDTLAPVSDDGETLGEIM
                     FRGNICMKGYLKNERATEAAFRGGWFHTGDLGVRMPDGYIRIRDRSKDIIISGGENIS
                     SIEVEDTLYRHPAVSVAAVVAMADPKWGEVPCAFIELKEGAQVSAEEIIAHCRLFLAG
                     YKLPKAVRFGELPKTSTGKIQKFELRARIKAEGTE"
     gene            complement(11950..12174)
                     /locus_tag="VHV99_29375"
     CDS             complement(11950..12174)
                     /locus_tag="VHV99_29375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639521.1"
                     /translation="MQNKHIHHYNGYAVKPSAHRLPDGSFSSNLLLERADSARAEGRY
                     QFYSLDYFTNEEQALQHSARWARHWVDTRG"
     gene            complement(12466..12669)
                     /locus_tag="VHV99_29380"
     CDS             complement(12466..12669)
                     /locus_tag="VHV99_29380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX3639522.1"
                     /translation="MDTGTVKWFNDSKGFGFITPDKGGDDLFAHFSEISGDGFKTLAE
                     NQKVSFETKQGPKGLQAANIKPM"
     gene            complement(12979..13614)
                     /locus_tag="VHV99_29385"
     CDS             complement(12979..13614)
                     /locus_tag="VHV99_29385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012338617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEX3639523.1"
                     /translation="MPASTAIITILAALLLGAMSPGPSFVIVARNSIGLSRGDGLATA
                     VGMGIGGVFFSGIALLGLYTLLATVEWLYVGLKVAGGLYLIYLASKIWRGAAKPLAFD
                     ATQAAATNVRKSFWIGLSTQLSNPKTAVYYGSIFAALLPQHRPLWCYFALPPAIFAIE
                     AGWYTVVALCFSSKRPREIYLRWKGWIDRVAASAVIALGLRLILTAHKVGI"
     gene            14151..14414
                     /locus_tag="VHV99_29390"
     CDS             14151..14414
                     /locus_tag="VHV99_29390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HEX3639524.1"
                     /translation="MKEREEQRLFDLDGNARERLIVWIRRRMDDYGITLEALADSIEA
                     DKAAIPVVKYRDAFGNTWDGHGDKPDWLARAIYAGQNIDHFRC"
     gene            complement(14681..15268)
                     /gene="pcaG"
                     /locus_tag="VHV99_29395"
     CDS             complement(14681..15268)
                     /gene="pcaG"
                     /locus_tag="VHV99_29395"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014899603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HEX3639525.1"
                     /translation="MTTLKQTPSQTVGPYFAYGLCPQQYDFDFRSLFTPVLADREAAG
                     EHITIVGQVFDGDGKVIGDAMLEVSQVDAQGHYPESREEILKTGFRGFARVGTGTDPQ
                     KRFVVETVKPGRASPDEAPHLNVIVTMRGMLLHTFTRVYFEDEAAANERDAVLAAVPA
                     DRRGTLIARREPDTANVYRFDIYMQGDKETVFFDL"
     gene            complement(15272..15976)
                     /gene="pcaH"
                     /locus_tag="VHV99_29400"
     CDS             complement(15272..15976)
                     /gene="pcaH"
                     /locus_tag="VHV99_29400"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014725036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HEX3639526.1"
                     /translation="MDESFLSARDFASHPAYVYPGYGSSVKRGPTRPLIPLKEKLRDQ
                     RVPVYGTEDLGALDHDLTRNAVRNGEPLGERIIVTGRVLDEGGRPVRNTLVEIWQANA
                     AGRYVHKADQHDAPLDPNFLGAGRCITDNEGRYRFLTIKPGAYPWGNHPNAWRPNHIH
                     FSLFGDHFGSRLVTQMYFPGDPLLAFDPIFQGTPEQARDRLIANFSLDTTQEAYALGY
                     DFDIVLRGRNETPMER"
     gene            16084..17136
                     /gene="pcaQ"
                     /locus_tag="VHV99_29405"
     CDS             16084..17136
                     /gene="pcaQ"
                     /locus_tag="VHV99_29405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pca operon transcription factor PcaQ"
                     /protein_id="HEX3639527.1"
                     /translation="MQRSLADSRVKFRHLQCFLAVAQFGGVQRAAESLSITQPAVSKT
                     VAELETILGVKLFERGRHGAVPTREGQLFMPHASACVSALRQGVDLLARAEGAAAATL
                     EIGILPTVAAALVPPVLKQFAALWPRVIVRLATGANPELLERLKAGTIEFAIGRLADP
                     ERMVGLSFEQLFSEPMIAVVRAGHPLALGAGLPATSLEDFPVVLPPFGTLIRQSADGL
                     LSAWGVPPLSGFVEVLSVSTGRALTLENDAVWFVPLSAVEYELAHGMLVRLPLPFAGT
                     GEPVGLIRRSDTQPSAVGRAFIDAVREVARQRMAAVAGKPVAKRASKRGRGSTPKAPK
                     THAATPRTLREPSSTK"
     gene            17317..18450
                     /locus_tag="VHV99_29410"
     CDS             17317..18450
                     /locus_tag="VHV99_29410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3639528.1"
                     /translation="MPSDLPTPDAALRAVSAPEHNPLGTAGLEFVEFASREPKALGET
                     FTKLGFKAIARHISKDVTLYRQGEMHFLINAEPDSFAERYAEEYGAGICAIGIRVANA
                     QRAFDRAIELGAWAFEGERLGAGELLIPAIQGIGDSHIYFVDRWRGRGGQRGGLGDIS
                     IFDIDFRPIEIDTAQADLNHAGTGLIAVDHLTQTVGEGRMQEWLDFYRDLLNFREIHE
                     LHANWHVSAESRVMVSPCGAIRVPLYEEGTRRTNLMHEYLPDHPGEGVQHIALATDDI
                     FACVERLLANGVEFVEPPPRYYEQLGARLPGHGLDVERLKRTHVLVDGEIAANGVPML
                     FFQTFVRRGAGEIFFEIVQRQGHHGFGEGNLSALAQAREQAGA"
     gene            18506..18751
                     /locus_tag="VHV99_29415"
                     /pseudo
     CDS             18506..18751
                     /locus_tag="VHV99_29415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006411605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1338 family protein"
     gene            18784..21801
                     /locus_tag="VHV99_29420"
     CDS             18784..21801
                     /locus_tag="VHV99_29420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HEX3639529.1"
                     /translation="MGTSLEEEQGAGVNALSSDAADSTSRRPRIAMPFSSTAAVMQAL
                     EADLRRNVRGEVRFDQGSKALYAADASNYRQVPLAVVVPSDVDDLLATLAACRRNDVP
                     FLPRGGGTSQNGQCVNVAVVADASKYVNRVVSVDPVARVAIVEPGVVCDTLRDAAEAH
                     GLTFAPDPATHSRCTLGGMIGNNSCGAHSVMAGKTVENVETLEIATFDGARFWVGPTS
                     DDELERIIRAGGRQGEIYAALKQLRDTYADQIRAKFPRIKRRVSGFNLDQLLPENGFN
                     VARALVGTEGTCAVTLQAKVRLVKSPAKRVIVVVGFTDIYTAADAVPHFMRCGPIAIE
                     GLDRAIIRGLQARGLKKDEIALLPEGDAWVVLEFGADTHADAVRQAETAAAYFLSGEA
                     GPNVSAIRVEDRALQAKVWSIRETGASAVALSVDSDMPDPVVGWEDAAVDPMRLGDYL
                     RAFQALVDRYGYETSLYGHFGDGCVHARITFDLRSVAGVATWRKFLREAAELVVEFGG
                     SLSGEHGDGQAKAEFLPIMYGAELMHAMEQFKAIWDPANRLNPGKVVHAYRADENLRM
                     GPAYKPVTLQTRLTFASQEGDGFQREIERCIGMGKCRSLEGGTMCPSYRATREEKYST
                     RGRAHLFWEMLQGDVIADGWQSREVKEALDTCLACKGCKSDCPTHTDMASYKAEFLAH
                     YYETNRRPRQALFMGRIGEWAPWAARFPRLTNFMTSAPGLAAVGKWLAGVAQARELPK
                     FAGQTYRQIARRAPQAARAAHSGDEVRKVILWVDTFNDHFTPEIAQAAADVLTQMGWH
                     VVLPKNRLCCGRPLYDFGLLERARELLTTILDDLGDDIAVGVPLVGLEPGCLSVFKDE
                     LLKQFPGHVLAKQLSAQTFLFSDFVARQPFEWPTLAADVVVHGHCHQKALFGMQGDTA
                     LLKKLGVKWTLLDTGCCGMAGSFGFNDEHHALSEKIGEDKLFPAVRAAAAANAETIVL
                     TNGFSCREQIQQGTGQHAMHIAQLAQRALAARC"
     gene            complement(21903..22535)
                     /locus_tag="VHV99_29425"
     CDS             complement(21903..22535)
                     /locus_tag="VHV99_29425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEX3639530.1"
                     /translation="MDTHLTKPADQSTTDLGRRVRAARQAQDLTLETASRLCGVSRST
                     LSKVENGLMSPTFDVLQKIVLGLKIEIGELFGSTPKVSASGRRALTRKNEGQRHAYRG
                     YQMELLATDLAHKAMLPFRIRISAHTLDAFDDWGRHEGEEFLYVISGSVCLYSELYAP
                     THLNAGDSLYFDSRTGHAAVSTSDEDAEVLWMATSADIPQTLATVESAKK"
     gene            22765..23727
                     /locus_tag="VHV99_29430"
     CDS             22765..23727
                     /locus_tag="VHV99_29430"
                     /EC_number="5.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyproline epimerase"
                     /protein_id="HEX3639531.1"
                     /translation="MTAMKTLTAIDSHTGGEPTRLVISGGPELGHGPLQERLAVFQRD
                     FDRVRAGVVNEPRGSDVMVGALLCEPHDPACSAGVIFFNNVGFLGMCGHGTIGLVVSL
                     AHLGRIEPGVHRIETPVGVVEAELHADRSVTVRNVPAYRYRHAVPLDVPGLGRVHGDI
                     AWGGNWFFLVSDHGQTLDHSRIDELTDATWAIRRALEANGITGEGGALIDHIELFADS
                     NRPGIDSRNFVLCPGKAYDRSPCGTGTSAKLACLAADGVLAPGALWRQESIIGSVFEA
                     HYDADPALPAGRIRPRIRGTAHVNAESRLVFSDDDPFAWGIRAS"
     gene            23724..24881
                     /locus_tag="VHV99_29435"
     CDS             23724..24881
                     /locus_tag="VHV99_29435"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3639532.1"
                     /translation="MKRSNGQPDVVVIGAGIVGAACAYELALQGLDVLVADRAGVGGG
                     ATAAGMGHLVVMDDSPAEFMLSAYSRALWLALAPRLDVRHAFVRSGTLWVAADDEEFA
                     LAQNRQRALQQHGIDCATLDAQQVREAEPALRHDLSGGMIVPDDAVVYAPAVAAWLLA
                     QPVAGRIVQRLDTEVIGIEREGVRLADGGLIAAGAVLLANGLQAATLLPSLPLQAKKG
                     HLLITDRYPGTIRHQILELGYIRSAHNQSGTSVAFNVQPRPTGQLLIGSSRQFDSVDP
                     RVEPAILARMLQRAVDYLPELAGLNAIRAWTGFRPASSDGLPLIGPAGFFADDAMHPA
                     TWLAVGHEGLGVTTALGTAKLIASQMLGQPAPFDASPYLPARFTHSGSVYA"
     gene            24874..25137
                     /locus_tag="VHV99_29440"
     CDS             24874..25137
                     /locus_tag="VHV99_29440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX3639533.1"
                     /translation="MHEPITLDIDGQHVRVTNGCTVAAAIAIAHRDATAVTRRSVSGE
                     LRGPLCGMGICQECRVTIDGVRHRLACQTICAAGMSVVTAGAA"
     gene            25137..26414
                     /locus_tag="VHV99_29445"
     CDS             25137..26414
                     /locus_tag="VHV99_29445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3639534.1"
                     /translation="MNVDVLVIGAGPAGLSAAQTASMHGATVGVVDDNALPGGQIWRQ
                     GPQYPASGTARALLEDLGARQNVALLSGARIVQALSAHALLAERDGRALTIGYRKLIV
                     ATGARERFLPFPGWTLPGVTGAGGLQALIKGGTPVRGERIVIAGSGPLLWAAATTARM
                     QGAHVVALVEQASSASVGRFARSLVRTPAKLLQAARMRAGLWRTPYLLDAYVSEATGD
                     ASVREVRVTQAGSDRLVACDRLACAYGLLPNTAIAAALGCRVDTQGQVDTIAVDEWQA
                     TSVPAVFAAGECTGVGGMELSAVEGKIAALAAVGESERARPFFAERARYRRFAQHMHE
                     AFELNPRLRMLPTPDTLFCRCEDVPYREMAQHTSWRDAKLHTRCGMGPCQGKVCGAAA
                     AFCFGWRRSGEQMSRPPFSPVRIETLLQIGSDV"
BASE COUNT         5163 a         7799 c         8490 g         5154 t
ORIGIN      
        1 agcgtcattc actgtgattc caccgtcatt cctgcaggac ctgctcaacc gcgtcgatat
       61 cgtcgacgtg gtcggccggt atgtgcagtt gaaaaagggc ggcgcgaact tcatggggct
      121 gtgcccgttt cacaacgaga aaagcccttc gtttaccgtt agtccgacta agcagttcta
      181 tcactgcttc ggctgcggcg cgcatggtac ggcgatcggc ttcctgatgg agcacgtcgg
      241 ttccacgttt ccggaggcgg tcaacgaact cgcgcaatcg gtcggattga ccgtgccgaa
      301 cgagccttcg ccgggatata gcggcggttc tggcgggtat tcgccggcgg cgtccaaggc
      361 ggtcaccacg gcgctatccg atgtcatgca gaccgcgtgc gatttttacc gcaaacagct
      421 gcggggcgcg ccggtggcga tccagtatct gaagaagcgc ggcctgactg gcgagatcgc
      481 cgcccgcttc ggtctcggct acgcaccgga cggctggcag aacctcgagc ccgcgttccc
      541 caactatcgg gacgacgcgc tggtcgagtc cggtctcgtg atcgtcagcg aaaaatcgga
      601 tgcgcagggg cagaatcgcc ggtacgaccg ctttcgcgag cggatcatgt tcccgattcg
      661 caatgtcaaa gggcaggtga tcggtttcgg cggccgtgta ctggacggcg gcgagccgaa
      721 atatttgaat tcgcccgaaa cgcctctatt taacaaaggc agcgagctgt atgggctgtt
      781 cgaggcgcgt ctggcgattc gcgaacagca ttacgtgctg gtggtggaag ggtatatgga
      841 cgtggtcgcg ctggcccaat tggggttcca gaacgcggtg gccacgctcg gcacggcctg
      901 tacgccgatt catgtgcaga aactgatgcg tcagaccgat acggtcatct tcagcttcga
      961 cggcgattcg gccgggcgcc gcgccgctcg ccgtgcgttg gatgcctgtt tgccgcatgc
     1021 ggcggacaac cggaccatcc gcttcctgtt tttgccgtcc gagcacgatc cggacagcta
     1081 tgtgcgcgaa ttcggcacgg aagcgttcgc cgagcaggtc gagcgtgcca tgccgctgtc
     1141 gcagttcatg ctgaacgaag tgctgaccgg caaggaactg gaccagcccg aaggccgggc
     1201 ccgcgcactg ttcgacgcga agcccctgct gcagcaactg ccggcgaatg cgttgcgcgc
     1261 gcagatcatg catatgttcg ccgaccggct cgacgtgccg ttcgacgagg tcgccgcgtt
     1321 atgcgaagtc gacgcccgga tcgcggcggt tgctcgttcc gcgcccgccc gcaaagaccg
     1381 gcgcagcgtg accgggatcg aagagaagac gctgcgcaat ctggtgatgt atccgcgcac
     1441 ggtagcggtt ctcgacgaag aagccgaaca ggcgcttctg agcgtcacgc ggcacggcga
     1501 gctgttcgag gaagtgacga cgcatgcacg cgcactgggc gattcggcgg agtttcagtt
     1561 gctgtcagac ctattgagaa acggcgcgaa tgccccaact ttcgaggaaa tctttcgcaa
     1621 aattctagac tatgatgaaa acgtccggga tttactactg aaggacccgg aggatgcgac
     1681 cgtcatcgag gagcggcgcg agcaggaacg gatcgtcgga gaggagctga aagcggcgat
     1741 tctgaagatg cggtatgacg cttattgcga tcgtctcgag cagctttcgc ggcaatcccg
     1801 gcacacgccg gaggagttca cggagttgac gagcctgaat cagaagcggg ctgaaatgaa
     1861 gcgccagctc gggctgtaag cgaggtggcg aaagccggag tgctataata aaaggtttct
     1921 agcggtttgt tttttcaatg gttttcctag caaaggcgaa aaggcgattg cgatggcaaa
     1981 gacaccaggc ggaaaaaaag caccaggcac aggttcgaac gaaccgacca gaaaggtcac
     2041 cgaggccaaa ttagcttctt cctccagaaa aacgtctgcc acgagcgtca cacctgctgc
     2101 cgggaagaaa ccctcgacca cttccgctgc gcgagcggag ccggtcaggg cggcgaaagc
     2161 tgtaccacca ccggccacaa aagggccggg tgtcagaagc gcaagggaag cgcctgccgc
     2221 gcaggacgat gcggtagagc gtgagactcc agtatccacg gttcaaccgg ctgttgtcca
     2281 acagccgcga gtcgagacta cagccggtac gacgaactcc atgacgaaaa agctgaacga
     2341 aacacccgtc gatgacgacg caacccaaag cgaagaaacc cctgctgccg ttccgggcaa
     2401 ggtggaaaag gtcaaggctc gcgatcgtcg tgctaaggaa aaagcgctgt tgaaagacgc
     2461 gttcgcgtcg tcgcagccag gcacggtcga ggaactcgaa gagcgccgct ccaaactgcg
     2521 cgcgctgatc aagctcggca aggagcgagg cttcctcacg tacgcggaaa tcaacgacca
     2581 cctcccggac aacttcaccg agaccgaggc gatcgaaggc attatcagca cgttcaatga
     2641 catgggcgtg gctgtgtacg agcaggcgcc ggacgctgaa acgctgctgt tgaacgacaa
     2701 cgcgcccgct gcttcatcgg acgacgaagt ggaggaggaa gcggaagtcg ccctttccac
     2761 cgtcgactcc gaattcggcc gcaccaccga cccggtccgc atgtacatgc gcgaaatggg
     2821 tacggtcgag ctgctcacgc gcgaaggcga aatcgaaatc gccaagcgga tcgaagacgg
     2881 cctcaagcac atggtgatgg cgatctccgc gtgcccgacc acgatcgccg acattctggc
     2941 catggctgag cgtgtcgcga acgaggaaat ccgtattgac gaactggtcg acggcctgat
     3001 cgacgccaac gcggaagatg cggacggctt ctcggcgcag gaagcggaag cgatcgaaag
     3061 cgaagacgag gaagtcgagg aagaggacgc ggacgacgag gaagaagacg acggcacggc
     3121 gcaagccacg gccaacgcag cgcagatgga agcgttgaaa cgtgcatcgc tcgagaagtt
     3181 cgccatgatc agcgaatggt tcgacaagat gcgtcgcgcg ttcgaaaagg aaggctacaa
     3241 gtccaagtcg tatctgaagg cgcaggaaac gatccagaac gaactgatga cgatccgctt
     3301 taccgcgcgc accgtcgagc gtctgtgcga cacgttgcgt gcacaagtgg acgaagtgcg
     3361 tcaggtcgag cgtcagattc tgcatacggt ggtcgacaag tgcggcatgc cgcgcgcgga
     3421 attcatcgcg cgtttcccgg gcagcgaaac ggacctggag tgggccgaca agatcgtcgg
     3481 tgaggggcac gcgtatagcg ggattctcac gcgtaacatt ccggccattc gcgaacagca
     3541 gcaacgtctg ttggatctgc aggcgcgcgt cgtactgccg ctgaaggacc tgaaggagac
     3601 caaccgtcag atggcggccg gcgaactgaa ggcgcgtcag gcaaagcgtg aaatgaccga
     3661 ggcgaacttg cgtctcgtga tctcgatcgc gaagaagtat acgaaccgcg gtctgcagtt
     3721 cctcgacctg attcaggaag gcaatatcgg cctgatgaag gcggtggaca agttcgaata
     3781 ccgtcgcggc tacaagttct ccacctatgc cacgtggtgg attcgtcagg ccatcacgcg
     3841 ttcgattgct gaccaggcgc gcacgatccg gattccggtt catatgatcg aaacgatcaa
     3901 caagatgaac cgcatctcgc gtcagatctt gcaggaaacc ggtctcgagc cggatccggc
     3961 aacgctggcc gagaagatgg aaatgccgga agacaagatc cgcaagatca tgaagatcgc
     4021 gaaggagccg atctcgatgg aaacgccgat cggtgacgac gacgattcgc atctgggcga
     4081 cttcatcgaa gataccaaca cggtggcgcc ggctgatgcc gcgctgcacg ccagcatgcg
     4141 cgatgtcgtg aaggatgtgc tcgactcgct gacgccgcgt gaagcgaagg tgctgcgtat
     4201 gcgtttcggt atcgagatga gcaccgatca cacgctcgaa gaagtcggta agcagttcga
     4261 tgtgacgcgt gaacggatcc gtcagatcga ggcaaaggcg ctgcgcaagc tgcgtcaccc
     4321 gagtcgctcg gacaagctga agtcgtttct cgtagggaat tgatccaggc tgccgggtcg
     4381 tgggaaagga agtagcgggt tgaagagggc cttccggttg tttcgcttaa gggaagcggt
     4441 tgcggaggcc cttttttcgt gtagtatgtc ggccaatcaa cgaattggcc cggccttcat
     4501 gaccgggtct ttttcttggg ccggacgccg tgctggttgc aggcagcgga ctcataatcc
     4561 gcctccgaaa ggacaccgtc ggttcgactc cgacccggcc caccatagtt gtaaagaagg
     4621 gttccgagtt ttcggaaccc tttttgtttg tgatgagaaa ccctatctga tgcggcaata
     4681 tgccacttcc gctgtgtcat taatccggtt ttgccgattc cggctcggct tgttagaaac
     4741 acttttgtcg cggcgttaag tggcttaaac gacattccgc attcattctc tctcgttcaa
     4801 atacacgtat taagcgccgc gttatccgtc ggcgatatcg cggttcgctt tatccatcac
     4861 tgcggcaaaa tctccgcggt ctaatcgcct ttttcctgct atttacaata tccggaccgt
     4921 gggccagtac agcatgttct tcgaggtaag gcgcacagac tcgcctgcgg catgtcgcta
     4981 atctcgtcaa ctgacactta gaagtccgct ccgcgtgcgt ggcacgtcgt ttgcataggc
     5041 tcggcttcca ggccacatca ataatgagaa ccactatgcg acccgaccac accgctcacg
     5101 attttgccca gccgcaacca gtggttgccg cgccgatgga agacgcagag gggagcccgc
     5161 cgcagaggcg gttcaatgca ttcacgcgcg tcaccttctg ttttgcatgg ctattcggcc
     5221 tggagccgtt tctgacgggg gccgtcgacg aagtgctgcg ctccaggggc gatcgccatt
     5281 aattgcgggt cacttaggac ataggcgacg ccgcatcgac ggacgttgaa agtttcgcgg
     5341 cgcaagaggg gcgattccta cggcccgggg gaggcggccg caacgcgcga ggcctaggcg
     5401 ctctcatgcg gcgaacggac tcgccgcgtg cgactcgccg tcgggaatgg gcgttgcgcc
     5461 tcggaaggtg ggacatcttc ccatctacag gacgcatatc atgcaaaccg accccaccat
     5521 cgatcccgtg gcggatcctg ccaaccaacc gctcgacgac ccggaagcgc agccgcgccc
     5581 attgcctggc acctcgccgg aaaccgatcc gccggcgcca aggaattcac ttgcggatga
     5641 cgaggcgctc cgctggtcgc ggacgtcttg ataacgacca tcgaccggga agtagggaaa
     5701 ctccgtgtga aaaaacgcac gcctgtgccg ttaaatgatt acggaaggct gttcgatgta
     5761 tgccgtccgc tttcagcacg ggtttttgtc acgcggcggt cggcgccgca actcacctcc
     5821 gggcagcgcc gtgttgaagg catcctcttc cccgcgggat tgccattgcg aaacgagcgc
     5881 tctgctggca aaagctacaa ctggactcca taaaaccgcc ggacggcggt tttttgtggg
     5941 gccctccgtt cacttgctcg tcgggctcgg ctcgcctcga tgcgcgccga cagatgccct
     6001 aggtggtcga ggaggagggc ggcatcggat caatctttgt atctgggcgc cgtaatctcg
     6061 gaataaagca ggcagccgtt cgtcatcatc tttaaccaca gctaatgcaa agattctgtt
     6121 gcgcggcgat tgagtgatta aatcaggcga ccccattaac tcaattaagt tcccgctttt
     6181 ctgaaacaaa tctactccgc gtcgaaatgg cgcattgcca tcgcctgatt ttcgagcaat
     6241 cagacggttc tcagttttca aacggtccga cccgattgcg acccgcatat taaatgctgt
     6301 tgcatacgca caactttgat attgatatgt tgcaaaaaaa ttatgtccag agccgccgtg
     6361 ccatcgcgct gattttgcga taaatgccta aaaaacaaag acttaatgtc tttgaggatt
     6421 tgattgttgc gaattatgaa aaatcctgtt gcgtaaatcg ccggcgtcgc taccagggtg
     6481 tccccctaca ctcacgcttc gttctaaggg tgcccggccg atccgaaacg atccggccaa
     6541 cagggcggca gtgagtgatg cattcgctgc tgaggcaggt cccgctcccc gcgtaaattt
     6601 atcgaactgg agtcccagca tgaaaaagag tctcatcgtc gttgcggttg ccgcatcgtt
     6661 cgcttccgtc gctcacgcac aaagcagcgt taccctgtat ggtctgttgg acgcgggcct
     6721 gacgtacacc agcaacgtcg cacctgccaa cggcgtcggt cacggcaacg caaagtgggc
     6781 agcgggtagc ggtggcatca accaaagcat gttcggtctg cgtggttcgg aagatctggg
     6841 cggcggtctg aaggcaatct tcacgttgga aagcggcttc aacgtcaaca acggcaagtt
     6901 cgctaacaac aacggcatgt tcaaccgtca agcgttcgtc ggtctgtcga gcgcacaatt
     6961 cggtacggtc acgctgggtc gtcaatacga tgcggcacaa gactacctgg caccgctgac
     7021 cgcaacgggc agctggggcg gtacgtactt tgctcacccg ttcaacaacg acaacctgaa
     7081 cacgaacggc ggctacgcag tcaacaactc gatcaagtac tcgagcgcta actacgctgg
     7141 cttcacgttc ggcggcacgt acggcttctc gaatcaggct ggcgcattcg caaacaaccg
     7201 cgaatacagc cttggtgctg cataccagtg gcaaggcctg cacctcggcg ctgcttacgc
     7261 acagcagaac aacccgggtg caacgaacgc agcaggcacg gcaagcaacg gcggcgcttc
     7321 ggacggctcg atcctgggct tggccggcaa cttccgtcag cgggaattcg gtgcagctgc
     7381 ttcgtactcg ttcggcccgg caaccgtcgg tgctgcatgg acacagtcgc gtatcgacaa
     7441 cgtcgttggc acgcaacaat cgctgagcgc taacaactac gaagttaacg gcaagtacaa
     7501 cctgaccccg gctctggcct tgggcgttgc ttacacgttc acggatggca agggctacgg
     7561 cgttgctgca gacggcggtt cggcctcggt gcgctaccac cagatcggcc tgcaagctga
     7621 ctactcgctg tcgcgtcgta ctgacgtcta cgctcaagcc gtgtaccagc acgcaatggg
     7681 cgacggcggt gtcgcttcga tctacagcgg cgacaacacg caatcgccgt cgtcgtcgaa
     7741 gaaccagacg gctgctacgg tcggtctgcg tcaccgcttc taagcttcag cagaagctgg
     7801 ttgttgcaga aaaggtgccg ttcgcggcac ctttttttat gggcgcgcca cgttggtgga
     7861 cgacacgcgg cgtgaatgtc agatgtcgcg cgcttgatga atctgcggga cgtacgtgac
     7921 ctggaaaatc gtacgcagac cgagtgcaac gccggtctgg ttgctcttgc ccggtgattc
     7981 gaccggagca gtacccaata tcttgtcgaa atcgaccgta ccgtagacct cggtgcgttt
     8041 tcgacaacac atactcagcc cgcgcctcga acgtatagcg attgccgcat tggacatgtg
     8101 gtcgtggtac gccttgccgg tcagcgtgag ggcgcgcgtg gtttgccact tcacggcggc
     8161 aaacgggccg tgattaattg tcagaatgag cgcgcggctc aatcacgcat gttgctcgac
     8221 cttgaacgcc gcaaccgcat ttcgcagacg ttcggtctgc tcatcgagtg aacctgccgc
     8281 ggccgctgcc tgctcgacta gtgcggcatt gcgctgcgtc acgtcatcca tttggcggat
     8341 ggcgagattg acttcgccga tacccgaact ctgctctttc gaagccgccg aaatttcgtt
     8401 catgatcgcc gtcacgcgcc gcaccgcgtc gaccacctcg ccgatcgtgc tgccagcctt
     8461 gcctgccagc gtcgagccct cgccgacctg cgcgaccgac tgatcgatca gcaccttgat
     8521 ctccttggcc gccgttgcgc tgcgctgcgc gagaatgcgc acttcgcccg cgacgacggc
     8581 gaagccgcgg ccttcctcgc cggcgcgcgc ggcttccacc gcggcgttca gcgccatgat
     8641 attggtctgg aacgcaatgc cttcgatcac actgatgatc tcgccgatct tgcgcgaact
     8701 gtcggtgatg ccggccattt tctcgaccac gtcgttgacg acctggccgc cgcgcgcggc
     8761 gatcgccgac gcatcggtag ccagttccga ttcggtggcc gcgctgtcgg cattctgcgt
     8821 gacggtcttg gtgagctcgt ccatggtggc ggccgtctgc tgcaacgaag ccgcctgttc
     8881 ttcggtgcgg ctcgacagat ccaggttgcc cgcggcaatc tctttcgagg cgaccgtgat
     8941 cgtctccgcg gatgtcttga tgcgcccgac cgtcgcggcg agttgactgc gcatctcgcg
     9001 cagcgagaac agcaggctcg acgtgtcgtc gcgacgcagg tcgatatgag tggcgaggtc
     9061 gccgcgtgcg atctgcattg caatcgccgc cgccacggaa ggctctccgc cgatcgagcg
     9121 cgatacgttg cgcaccacgc gcgaggccac gaacgacgcc agcgcaccca gcagcaccag
     9181 cataccggcc gagcgcgcga gcgtggcgta gaactgcgcc tggatgtcgt ccatataggc
     9241 gccggcgata aaatcccagt tccacgggac gaagcgtttc acatagccga tcttttcgct
     9301 cggcgctgtc tcgccgggtt tcggccacca gtagcgcaga tagcccgcgc cggctgccga
     9361 gccgcctgcc tgcgcgatgt cgtgatacag cgcgttgccc ttggcgtcgc gaaagccgct
     9421 catgtccttg ccgttcaggt tcgggctcat cggatgagcg accattaccg aatcgccgcg
     9481 cacgatcgtc acgtagcccg aagtgccata acgctgtgcg ttgatgcggg cgatggcctg
     9541 ctgtttggcc tcgtcgaccg ggagcttgcc cgtgtcggcc tgtttcgcgt agtccgcgac
     9601 gatcgatacc gccatgtcga tgacgtcggc cagatccgcg cgccgtgcgt ccatttgggt
     9661 gttgcgcgcg tcgaacgcgt tcactatggt cacgaccagg agcgccgccc agcagagcag
     9721 tatcggcacc cacaatttct gttttagtgt caggcggttc attatcggct tcggcagtac
     9781 attgaacttg gaattggaag gagctgcaat ctgcgttgca tagtcggcat aacggccttt
     9841 acgccggatt ctttattccg gcatccgaaa ttcctgccag gcgtgcgaat gtccgatatg
     9901 aggcggccgc tgaaaacggg aaacgtcggg gaaaattggg ggaaagtgga accaaatctg
     9961 gagaaaatcg cgggaaacca cggtggttag cccgcccccc aggccgcagc ccgccagctt
    10021 ggagcccgct agcagtcagc ccgcaacccg ccgcccgccg ccgacagccc accgcccgca
    10081 actcacagct ccctgccgtt gaatagagcc agagcgcgtc actctgtcat ttgaatgaga
    10141 gacagttcac catgacaatt gctaggctag atgcatcaat cagaaaaagc gacggcgcgg
    10201 ccagcgcctc aggagacaac gatgaagcac atgttcgaag aaggccttga gcggcgcgag
    10261 gccaattatg tcccgctgac gcccatcgat ttcatcgtac gcgcggcgga agtctacgga
    10321 gagcggctgg cggtcgtcca tggggagata cgccgtaact ggcgcgagac ctatgagcgc
    10381 gcgcgacgtt tggccagcgc gttacggcag gccggcatcg aacgcggtga cacggtggct
    10441 gccttgctgc cgaatattcc tccgatgata gaagcacact tcggtgtgcc gatggccggc
    10501 gcagtgctca atacgctgaa cacgcgcctt gacgtctcgt cgctgctgtt catgctgcgc
    10561 catggcgagg cgaaggcgtt gatcgtggat acggagtaca gcgaattggc gcaccgcgcg
    10621 gcgcaggaat tccccgagtt acgcgtgatc agcgtggcgg acgcaatgcc cgccgacgcc
    10681 gacagattca tccgcgcgac cgactacgaa gcgttcctgc aatccggcga ccctgaattc
    10741 gcgtggaccc tgccagccga tgagtgggac gccatcgcgc tgaactacac ctcgggcacg
    10801 acgggcgatc cgaagggcgt ggtctaccac catcgcggcg cctatctgaa tgcgctcagc
    10861 aatatcctcg aatgggatat gcccaggcat gcggtctacc tgtggacctt gccgctcttt
    10921 cactgcaacg gctggtgttt cccatggacc gtcgccgcgc gtgccggcgt caacgtctgt
    10981 ctgcgcaaat tcgacgcgaa gacggtgttc gatctgatcc gccacgtagg catcacgcac
    11041 tattgcggcg cgccgatcgt gcaaagcgcg ctcgccaatg cgccggccga gtggcgtgag
    11101 ggcatcacgc atcgggtgtc gacgatggtg gcgggcgccg cgcccgcgcc ggcagtgatc
    11161 gccaggatga aggagatcgg cttcgatctg acgcatgtgt acggactcac cgagacctac
    11221 ggcccggccg cggtctgcgc gaaacaggag gaatgggaaa cgctcgacga cagcgcacgc
    11281 gctgaaatga acgcgcggca aggcgtgcgc tatcacctgc aggcagccgt aacggtgctc
    11341 gacccggaca cactcgcgcc cgtgtctgac gacggcgaaa cgcttggcga gatcatgttc
    11401 cgcggcaaca tctgcatgaa aggctatctg aagaacgagc gcgccacgga agcagccttc
    11461 cgcggcggct ggttccacac cggtgatttg ggcgtgcgga tgcctgacgg ctatatccgc
    11521 atccgcgatc gcagcaagga catcatcatt tcgggcggcg aaaacatctc gagcatcgag
    11581 gtcgaagaca cgttgtatcg tcatcctgcg gtgtcggttg ccgccgtggt ggcgatggcc
    11641 gatccgaaat ggggcgaagt accgtgcgcc ttcatcgagc tcaaggaggg cgctcaggtg
    11701 agcgcagaag agatcatcgc gcactgccgg ctattccttg cgggatacaa gttgccaaag
    11761 gcggtgcgct tcggcgaatt gccgaagacg tcgacgggaa aaattcagaa gttcgaattg
    11821 cgcgcgcgga tcaaggcgga aggaaccgaa tagatcgcgc gcgattgtat agcacaccca
    11881 atggcgcgct atatggtgct gaatgatgca agcgccggtg tcccgacgcg catcagggtc
    11941 gactcgcgct taaccgcgcg tgtcgaccca gtggcgtgcc cagcgtgccg agtgctgcag
    12001 tgcctgttct tcgttcgtga agtagtcgag cgaatagaac tggtagcgcc cttcggcacg
    12061 cgcgctgtca gcgcgttcga gcaacaggtt ggatgaaaag ctaccgtcgg gcaaacgatg
    12121 cgcagagggt ttgacggcat agccgttgta atgatgaata tgtttgtttt gcattttgta
    12181 ttttaataat gttcgagtgc gcgcctgacg tgcgaaccgt tgtacgggtc acgcggacga
    12241 gccgattcaa ggcattgcga gccgaatcca aacgcagtcg gaaaggtaaa aagggcgttg
    12301 aagccagagg gggagaatga ggtgcaacga ggcgtctggc gcttgagcta gttgcttttg
    12361 aaatacatgc aaccaaaaag caatcgcgcc aactgtggga gacaaagctc cgcgaggatg
    12421 tcgctgcagc ccgatgtccg ttgccggaca ccgagccctt caaacttaca tcggcttgat
    12481 gttagccgct tgcagaccct tcgggccttg cttcgtttcg aagctcacct tctgattttc
    12541 agcgagcgtc ttgaagccgt cgccgctgat ttcggagaag tgcgcgaaca gatcgtcgcc
    12601 gcccttgtcc ggggtgatga agccaaagcc tttgctgtcg ttgaaccact taacggtacc
    12661 ggtatccatg aagaatcctt gaggtaaaaa tagaaaaatt tgctctgttg gggagcgcgt
    12721 gaagcgtcaa ggagggagag gacaacgaat accgctgagg agcgagacat tgatgaacag
    12781 caatcgaact tcttgaactt cgggtgccac agtaaccgcc ggggcctatc agcgtcaaca
    12841 cttatcttgt aactgttttg ctgcgggttg ttgcgggctt tatgtcgctc aggccgagtg
    12901 catggcgata cgaccctcgt caccagcata tcgtgtcttg tggcacgaca cgatatgccg
    12961 tgcaagcggg ttgcagtttc agatccccac tttatgtgcc gtcaggatca ggcgcaatcc
    13021 aagcgcgatc actgcgcttg cggcgacgcg atcgatccag cccttccacc gcaggtagat
    13081 ttcacgcggc cgtttgctcg aaaagcacaa ggcgaccacg gtgtaccagc cggcttcgat
    13141 ggcgaagatc gcagggggca atgcaaagta gcaccacagc ggccgatgtt gcgggagcag
    13201 ggccgcgaag atactgccgt agtagaccgc tgttttcgga ttgctgagtt gagtgctcaa
    13261 accaatccag aaggatttgc gcacattggt ggcggccgct tgcgtggcat cgaacgcgag
    13321 tggtttggcc gcaccgcgcc agatcttcga cgcaagatag atcagataaa gtccgccggc
    13381 cactttcaaa ccgacataca gccactccac agtcgctagc aaggtgtaca gaccgagcaa
    13441 ggcgatcccg ctgaagaaca ccccgccaat gcccatgccg accgccgttg ccaggccgtc
    13501 gccgcgcgac agtccgattg aattgcgggc aacgatgacg aagctgggtc cggggctcat
    13561 ggcaccgagc agtagtgcgg ccagaattgt gatgatggct gtcgatgcgg gaattcttgt
    13621 gtctcctgtg ggcggtgatg cgggtcggtt aaacatggca tagagggtgc gggtttgttc
    13681 ttttcgcccc tgtggtgctg cctgctgtca ttctacagcg cttccattgc cctgctttgc
    13741 tgtcgggtta ttagcattgc ctcacacgag gccagcacta atagaaccac taataattga
    13801 ataagccctg ccgaacgcaa acagaacaac caggaaagcc cctcatggcg ggcaaacaat
    13861 cagttgcttc aagcagcgga aattggatgc tagaatcgct ttccatacgc tcaagcagcc
    13921 ttgcagcacc ggggccgatt acgagcgcga ccgaattgtt tgccgtcgaa ttcccctgaa
    13981 atcctggatt tattgggcat gatctccagc tgcaagttgt cccgcatctg gagaggcatc
    14041 gtgagttcga tttgcactca acccatctga ttcacgcata ggcttcgcgt tgtttagcga
    14101 agcctttgtt ttgccaacgc gagcactatc agccgcgaaa ccggtacgtt atgaaagagc
    14161 gagaagaaca acggctgttc gacctcgacg ggaatgcgcg tgagcgcctg atcgtgtgga
    14221 tccgccgacg tatggacgac tacggcatta cgttggaagc gctagcggat tcaatcgaag
    14281 ccgacaaagc cgccatcccg gtcgtgaagt atcgcgatgc cttcggtaat acgtgggacg
    14341 gtcatggcga taagccggat tggctcgcgc gcgccatcta cgccggacag aacatcgacc
    14401 atttccgttg ctgagcgtgg cttgaacctc gcgcaatgaa ggacaggctg accgggtcat
    14461 gccgattgct tcgatatcca aactgaccac cgcggacatg acgagattcc gcagccgtct
    14521 gcagcgttcg ttgaaatgaa cgattctgac gcgcagacgc gaaaaccaaa aaaaagcccg
    14581 cgcgcggcgg gcttaaccta ctcggagttt cgcgatccag tcgctagacg gatcctctcc
    14641 agtgtatgtg aaagctatct gaaagtgtag aaaatccgcg tcaaaggtcg aaaaagacgg
    14701 tttccttgtc gccctgcatg tagatatcga accggtacac attggccgta tccggttcgc
    14761 ggcgcgcgat cagcgtgccg cgccggtccg ccggaaccgc tgccagcacg gcatcccgtt
    14821 cgttagccgc ggcttcgtcc tcgaaataga cgcgcgtgaa ggtgtgcagc agcatgccgc
    14881 gcatcgtcac aatcacattc agatgcggcg cttcgtcagg gctggcgcga ccgggtttca
    14941 ctgtttcaac tacaaagcgc ttttgcggat ccgtccccgt gccgacgcgg gcgaagccgc
    15001 ggaaacctgt cttcaggatt tcctcgcgcg attccgggta gtggccttgc gcgtccactt
    15061 gcgacacttc cagcatggca tcgccgatca ccttgccgtc gccgtcgaac acctggccga
    15121 caatcgtgat gtgctcgccg gccgcttcac ggtcggccag aaccggcgtg aacaggctcc
    15181 tgaagtcgaa gtcgtattgc tgcgggcaca gaccgtaggc gaagtagggt ccaaccgttt
    15241 gcgaaggcgt ttgcttgagg gtcgtcatgg cttagcgctc catcggggtt tcgttgcggc
    15301 cgcgcagcac gatgtcgaag tcgtagccga gcgcataggc ctcttgcgtc gtgtcgagcg
    15361 agaagtttgc aatcaggcgg tcgcgggcct gttccggcgt gccctggaaa atcgggtcga
    15421 aggccagcag cggatcaccc gggaagtaca tctgcgtgac gaggcgcgag ccgaaatggt
    15481 cgccgaacag cgagaaatga atatggttgg gacgccatgc gttcgggtga ttgccccaag
    15541 ggtaggcccc cggtttgatc gtcagaaagc ggtaacggcc ttcgttgtcg gtaatgcagc
    15601 ggccggcgcc gaggaagttc gggtcgagcg gggcgtcgtg ctgatcggcc ttgtgcacgt
    15661 agcggccggc agcgttggct tgccagatct cgactaacgt attgcgcacc ggacggccgc
    15721 cttcgtcgag cacgcggccg gtcacgatga tacgttcgcc aagcggctcg ccattgcgca
    15781 ccgcattgcg cgtcagatcg tgatcgagcg cgccgaggtc ttccgtgccg taaaccggca
    15841 cgcgctggtc gcgcagtttc tccttcaacg gaatcagcgg gcgcgttggg ccgcgtttga
    15901 ccgaagaacc atagccggga tagacgtaag cggggtggga cgcgaaatcg cgtgcggaga
    15961 gaaaggactc gtccatcagt gtctccttcg aggaattgtg agatacagcc tatgaagcga
    16021 ctttatctaa actggccggt tatgcaaaat gacgttttca cccttttcgt ataacctgcc
    16081 gttatgcaac gcagcctcgc ggacagccgc gtcaaattcc gtcatcttca gtgctttctg
    16141 gccgtcgccc agttcggcgg tgtgcagcgg gcggcagaaa gcctgtccat cacgcagccc
    16201 gcggtctcca agacggttgc tgaactggaa acaattctcg gtgtgaagct gttcgagcgc
    16261 ggccgtcacg gtgctgtgcc gacccgcgag gggcagctct tcatgccgca cgcgagcgcc
    16321 tgtgtcagcg cgttgcggca gggcgtcgac ttgctggcgc gcgcggaagg cgcagccgcc
    16381 gccacgcttg agatcggcat actgccgacc gtcgcggcgg ccttggtgcc gccagtgttg
    16441 aagcagtttg ccgcactttg gccgcgcgtg atcgtgcgcc ttgccactgg cgccaacccc
    16501 gaattgctcg aacgcctgaa ggccggcacg atcgaattcg cgatcggacg tctggcggac
    16561 ccggaaagga tggtcgggct gagtttcgaa cagctcttca gcgagccgat gattgccgtg
    16621 gtccgcgccg ggcatccgtt ggcgttgggg gccggcttgc cggccacgtc gctggaggat
    16681 tttccggtgg tcctgccacc gttcggcacg ctgatccggc aatcggctga tggcctgttg
    16741 agcgcgtggg gcgtgccgcc gttgtccggg tttgtcgagg tgctgtcggt gtcgacgggg
    16801 cgtgcgttga cgctcgagaa tgacgcggtg tggttcgtgc cgctgagtgc agtcgaatat
    16861 gaactggcgc acggaatgct ggtgcgtttg ccgttgccgt tcgctgggac gggcgagcct
    16921 gtggggctga tccggcgttc tgacacgcag ccgtcggcgg tgggccgtgc gtttatcgat
    16981 gccgtgcgcg aggttgcgcg gcagcgcatg gcggcggttg cgggcaagcc tgtggccaaa
    17041 cgtgcatcga aacgtggccg cggcagcacg ccgaaggccc caaagaccca cgctgcaaca
    17101 ccacgtacct tgcgtgaacc atccagtaca aaatgaaggt tgcgaacgcc ctgctacccg
    17161 gtgtacaaac acggtgcgaa agccgtcgcg cggcgcattg ctgcggcaca ccacgcaagc
    17221 ggtgtcacac tacgagtgat tgacggtacg caccgagcag cagaaacatc acgaacacgc
    17281 gcaacagcgt acgaaagact gtacaaggag attgccatgc ccagcgactt gcccactcca
    17341 gacgccgcgt tgcgcgccgt gtcggcgcct gagcacaatc cgctcggcac cgccggcctc
    17401 gaattcgtgg agttcgcctc gcgcgagccg aaggcgctcg gcgagacctt tacgaaactc
    17461 gggttcaagg cgattgcgcg ccatatcagc aaggacgtca cgctgtatcg ccagggtgag
    17521 atgcacttcc tgatcaacgc ggagccggat tcgttcgccg agcgctacgc ggaggaatac
    17581 ggcgccggca tctgcgcgat cgggattcgc gtggccaacg cgcagcgcgc cttcgaccgc
    17641 gcgatcgaac tcggcgcgtg ggcgttcgaa ggcgaacgcc tcggtgccgg cgaactgctg
    17701 attccggcga ttcagggcat tggcgattcg cacatctatt tcgtcgaccg ttggcgcggc
    17761 cgcggtggcc agcgtggcgg tctcggcgac atctcgatct tcgacatcga cttccgtccg
    17821 atcgagatcg acaccgcgca agccgatctg aatcacgcgg gcaccggtct gatcgcggtc
    17881 gatcatctga cacaaacggt cggcgagggc cgcatgcagg agtggctcga cttctatcgt
    17941 gacctgctca acttccgcga gattcacgaa ctgcacgcga actggcatgt gtcggcggaa
    18001 tcgcgcgtga tggtgtcgcc gtgcggcgcg atccgcgtgc ctttgtatga agagggcacg
    18061 cgccgcacga atctgatgca cgagtatctg ccggatcatc cgggcgaagg cgtgcaacac
    18121 attgcgcttg ccaccgacga catctttgca tgcgtcgagc ggctgctggc gaacggcgtc
    18181 gagtttgtcg agccgccgcc gcgctattac gagcaactcg gtgcgcgtct gccgggccat
    18241 gggctcgatg tcgagcggct caagcgcacg catgttctgg tggatggcga gatcgccgcc
    18301 aatggcgtgc cgatgctgtt ctttcagacc ttcgtgcgac gcggcgccgg cgagatattc
    18361 ttcgagatcg tgcagcggca agggcatcat ggctttggcg aaggcaattt gagcgcgttg
    18421 gcgcaggcac gcgagcaagc cggcgcttag catttgccgc taaccgctaa ccgctaaccg
    18481 ctaaccgcta accgctaacc gctaaccgct cactgcggca cacacgtcgc tgccggtgat
    18541 cgtcggcgcg ttcgcgcgtc agcacgagac gccacgcgaa accgattacg aagtattgtc
    18601 gaaggagtcg gcggaaatgg cgcggatcgc gcccgagggc aacgccttca atcacgccac
    18661 cgatcgcgtc gcggacgtgt tcgcactctc cgacgacgaa aaggcgaagg gtcgtccgat
    18721 gaaggaagtc gatcgttcgc gttcgggccg cgcgggcgtt ggacacgaga catgaggaat
    18781 gcagtgggaa cgtcattaga ggaagaacaa ggcgcgggtg tgaatgctct gtcgagcgac
    18841 gcggctgata gcacgtcaag aaggccgcgc atcgccatgc ccttttcgag taccgccgcc
    18901 gtgatgcagg cgctcgaagc cgacctgcgt cgcaatgtgc gcggcgaagt gcgtttcgat
    18961 cagggctcga aagcgctgta tgccgcggat gcgtcgaact accggcaagt cccgctcgcc
    19021 gtggtggtgc cgtcggacgt cgacgatctg ttggcaacgc tcgctgcctg ccgtcgcaat
    19081 gacgtgccct ttctcccgcg cggcggcggc acctcgcaga acggccaatg cgtgaacgtc
    19141 gcggtggtgg ccgacgcgag caagtatgtg aaccgcgtcg tctcggtcga tcctgtagcg
    19201 cgtgtggcga tcgtcgaacc gggcgtggtg tgcgacacct tgcgcgacgc cgccgaagcg
    19261 cacggcctca cgttcgcacc cgatcccgcg acgcacagcc gctgcacgct cggcggcatg
    19321 atcggcaaca actcgtgcgg cgcgcattcg gtgatggccg gcaagacggt cgaaaatgtc
    19381 gagacgctgg aaatcgcgac cttcgacggc gcgcgtttct gggtcggccc tacttcggac
    19441 gacgaactcg aacgcatcat cagagcaggt ggccgtcagg gcgaaatcta cgcagcgctc
    19501 aagcaactgc gcgacacgta cgccgatcag atccgcgcga aattcccgcg gatcaagcgg
    19561 cgcgtgtcgg gctttaacct cgatcaactg ctgccggaaa acggctttaa cgtcgcgcgt
    19621 gcgctggtcg gcaccgaggg cacctgcgcc gtgaccttgc aggcgaaagt gcgcctcgtg
    19681 aagagtccgg ccaaacgcgt gatcgtcgtg gtcggcttca ccgatatcta caccgcggcg
    19741 gacgccgtgc cgcatttcat gcgctgtggt ccgatcgcga tcgagggact cgaccgcgcg
    19801 atcatccgcg gcttgcaggc gcgcggtttg aagaaggacg agatcgcgct gttgccggaa
    19861 ggcgatgcat gggtcgtgct cgaattcggc gcggatacgc acgcggatgc ggtacgtcag
    19921 gcggaaactg ccgcggcgta tttcctgtcg ggtgaagcgg ggccgaatgt ctcggcgata
    19981 cgcgtcgaag atcgcgcgtt gcaggcgaag gtgtggtcga tccgcgaaac gggcgcgtcg
    20041 gcggtggcgt tgtcggtgga ttcggacatg cccgatccgg tggtcggctg ggaagatgcg
    20101 gcggtcgatc ccatgcgcct cggcgattat ctgcgcgcgt ttcaggcttt ggtggatcgt
    20161 tacggctacg agacgagcct gtacggccat ttcggcgatg gctgcgtgca cgcgcgcatt
    20221 accttcgatc tgcgcagcgt cgcaggcgtc gcgacgtggc gcaaatttct gcgcgaggca
    20281 gcggaactgg tggtcgaatt cggtggctcg ctctcgggcg aacatggcga cggccaggcg
    20341 aaggccgagt tcctgccgat catgtacggc gctgaactga tgcacgcgat ggagcagttc
    20401 aaggcgatct gggacccggc caatcgactg aatccgggca aggtcgtgca cgcctatcgc
    20461 gccgatgaaa acctgcgcat gggtccggcg tacaagccgg tgaccctgca aaccaggctc
    20521 acgttcgcca gccaggaagg cgacggcttt cagcgcgaga tcgaacgatg catcggcatg
    20581 ggcaaatgcc gctcgctcga aggcggcacg atgtgcccga gctatcgcgc gacgcgtgaa
    20641 gagaagtatt cgacgcgtgg ccgcgcgcat ctgttctggg aaatgctgca aggcgacgtg
    20701 attgccgatg gctggcaaag ccgcgaagtg aaagaagcgc tcgatacgtg cctggcctgt
    20761 aagggctgca aatccgattg cccgacgcat acggatatgg cttcatacaa ggcggaattt
    20821 cttgcgcatt actacgagac gaatcgccgg ccacgtcagg cgctgtttat ggggcggatt
    20881 ggtgagtggg cgccgtgggc ggcgaggttt ccgcggctga cgaattttat gacgtcggcg
    20941 ccggggttgg ctgcggttgg caagtggctt gccggggtgg cgcaagcgcg tgagttgccg
    21001 aagtttgctg gacagacgta ccggcagatc gcgcggcgcg cgcctcaggc tgcgcgtgct
    21061 gctcactctg gcgatgaggt caggaaagtc atcctgtggg tcgatacctt caacgaccac
    21121 ttcacgcctg agatcgcgca ggccgcggcc gatgtgctga cgcaaatggg ctggcatgtc
    21181 gtgctgccga agaatcgttt gtgctgtggt cgtccgctgt atgacttcgg attgctcgag
    21241 cgtgcgcgcg aattgctgac cacgattctc gatgacctcg gggacgatat tgccgtcggt
    21301 gtgccactgg tcggtctcga accgggttgc ttatcggtct tcaaggatga gttgttgaaa
    21361 caattccccg gtcatgtttt ggcgaagcag ttgtcggcgc agacttttct gttttccgat
    21421 ttcgtcgcac gtcagccgtt cgagtggccg acccttgcag ccgacgtggt cgtgcatggg
    21481 cattgtcatc agaaggcact gtttggcatg caaggcgata ccgcgctgct gaagaagctc
    21541 ggggtgaagt ggacgttgct cgatacggga tgctgtggga tggcgggatc gttcggcttt
    21601 aacgacgagc atcacgcgct ttcggagaaa atcggcgaag acaagctgtt tccggctgtg
    21661 cgcgctgctg ccgcggcgaa cgcggaaacg atcgtgctga cgaatggttt cagttgtcgt
    21721 gagcagatcc aacagggaac cgggcaacat gcgatgcata tcgcgcaact ggcgcaaagg
    21781 gcgttggcgg cgcgttgtta gcgggctggg gagatgacgt tgccattggc gttgacttcc
    21841 gctttttttg cgttgcgttg cgttgcgttg cgttgcgttg cggcgccgcg cagacgcctt
    21901 cgctatttct tcgccgattc gacagtggcc agggtctgcg gaatatcagc gctggtcgcc
    21961 atccacaaca cttcggcgtc ttcgtcgctg gtggataccg ccgcgtggcc ggtgcggctg
    22021 tcgaagtaca ggctgtcgcc ggcgttcaga tgcgtcggtg cgtacaactc ggaatacagg
    22081 cacacgctgc cgctgatcac gtacaaaaat tcttcgccct cgtgacggcc ccagtcgtcg
    22141 aacgcgtcga gcgtgtgcgc cgagatgcgg atgcggaacg gcagcatcgc tttgtgcgcg
    22201 aggtcggtgg cgagcaactc catctgatag ccgcgatagg cgtggcgttg gccctcgttt
    22261 ttacgcgtca acgcgcggcg gccgctcgcg ctgactttgg gcgtcgaacc gaatagctcg
    22321 ccgatttcga tcttcaggcc gaggacgatt ttctgcagga cgtcgaaggt gggcgacatc
    22381 aggccgtttt cgactttgga gagggtggag cgcgagacgc cacagaggcg gctcgcggtt
    22441 tccagcgtca agtcctgcgc ctggcgggcg gcgcgcacgc gccggccgag atcggttgtc
    22501 gattgatcgg cgggtttggt gaggtgggtg tccatggggc ttgctgcttc aaatgcacgc
    22561 tttgaggggc gatcggtgtt tccgataata acatttggct ggtgccgccc tggttgccga
    22621 gcgtttcgtc cgaccatcga gcaacttttc tcatcggaaa cgtgacgcgt cgagattgat
    22681 atgcggtcag tggccatgtt gtgccgaaat cgtcatcgaa accggggcgt tagcgcaaga
    22741 cagcgctgag cgccacgtcc tacgatgact gccatgaaaa cactgaccgc aatcgactcc
    22801 cacaccggcg gtgaacccac ccgcctggtg atttccggcg gccccgaact gggtcatgga
    22861 ccattgcagg aacgccttgc cgtgttccag cgtgatttcg accgcgtccg cgcgggcgtc
    22921 gtcaacgagc cgcgcggctc cgacgtcatg gtcggcgcgc tgctgtgcga gccgcatgat
    22981 ccggcttgtt cggcgggcgt catcttcttc aacaacgtcg gctttcttgg catgtgcggg
    23041 cacggcacga tcggcctcgt ggtctcgctg gcccatttgg gccggatcga gccgggtgtg
    23101 caccggatcg agacgccagt gggcgtcgtc gaagctgaac tgcatgccga tcgcagcgtc
    23161 accgtgcgca acgtgccggc gtatcgctat cgtcatgcgg tgccactcga tgtgcccggc
    23221 cttggtcgcg tgcacggtga tatcgcgtgg ggcgggaact ggtttttcct cgtgtccgat
    23281 catgggcaga cgctggatca cagccgtatc gacgaattga cggacgccac ctgggcaatc
    23341 cggcgggcgc tggaggccaa cggcatcacc ggcgaaggcg gtgcgctgat cgatcacatc
    23401 gaactgttcg ccgattcgaa tcggcccggc atcgatagcc gcaatttcgt gctgtgcccc
    23461 ggcaaggcct atgaccgctc gccgtgcggc accggcacga gcgccaagct cgcctgcctc
    23521 gcggcggacg gcgtgctggc gccgggcgcg ctgtggcgcc aggagagcat tatcggcagc
    23581 gtgttcgagg cgcattacga tgcggatccg gcgctccccg cagggcggat ccggccgcgc
    23641 atccggggca ccgcgcacgt caatgcggaa tcccggctgg tgttttccga cgacgatcct
    23701 ttcgcgtggg gcatccgcgc gtcatgaagc ggtcgaacgg tcagccggac gtcgtggtga
    23761 ttggcgcggg aatcgtcggt gctgcttgtg cctacgagct ggcattgcag ggcctcgacg
    23821 tgctggtggc cgatcgtgcg ggcgtcggcg gcggcgcgac ggcggccggc atgggccacc
    23881 tcgtggtgat ggacgattcg cctgccgagt tcatgctcag cgcctattcg cgcgcgctgt
    23941 ggctcgcgct ggcgccgcgc ctcgatgtgc gtcatgcgtt tgtgcgcagc ggcacgctat
    24001 gggttgccgc cgacgacgag gagttcgcgc tggcgcaaaa ccgccagcgc gcgctacaac
    24061 agcacggcat tgattgcgcg acactcgacg cgcagcaagt gcgcgaggcc gagcccgcgt
    24121 tgcggcacga cctgagcggc ggcatgatcg tgcccgacga cgcggtggtc tacgcgccgg
    24181 ccgtggcggc ctggttgctc gcgcaacctg ttgccgggcg gatcgtgcaa cgtctggata
    24241 ccgaagtaat cggcatcgaa cgtgaaggcg tgcgtctcgc ggacggcggc ctcatcgccg
    24301 ccggcgccgt gctgctggcc aacggcctgc aggcggcgac actgttgccg agcttgccgt
    24361 tgcaggcaaa gaaaggtcat ctgctgatta ccgatcgcta ccccggcacg atccggcatc
    24421 aaatcctcga attgggttac atcaggagcg cgcacaacca gagcggcaca tcggtcgctt
    24481 tcaacgtgca gccgcgccca accggccaat tgctgatcgg ctcgtcgcgt cagttcgaca
    24541 gcgttgatcc gagggtcgaa ccggcgattc tcgcgcgcat gctacagcgt gcagtcgact
    24601 acttgccgga attggccggc ctgaatgcca ttcgcgcgtg gaccggtttt cgtccggcat
    24661 ccagcgacgg tttgccgttg atcggcccgg ccggattctt tgcagacgac gcaatgcatc
    24721 cagctacgtg gcttgcggtt ggacatgagg gattgggcgt gaccaccgcg ctcggtacgg
    24781 cgaagctgat cgcgtcgcag atgctgggcc aaccggcgcc gttcgacgca agcccttatc
    24841 tgcccgcacg gtttacgcac tcggggagcg tctatgcatg agccgatcac actcgacatc
    24901 gacggacagc acgtgagggt gacaaacggc tgcacggtgg ccgccgctat cgccatcgct
    24961 catagggacg cgacggccgt gacacgtcgc tcggttagcg gcgaattgcg cgggccgctc
    25021 tgcgggatgg gtatttgcca ggagtgccgg gtgacgatcg acggcgtacg tcatcggctc
    25081 gcctgccaga cgatctgtgc agcaggtatg tcggtagtca ccgcaggagc ggcatgatga
    25141 atgtcgacgt gctcgtgatt ggcgccggcc ccgccggact cagcgccgcg cagaccgcgt
    25201 cgatgcatgg tgcaacggta ggcgtcgtcg acgataacgc gttgccaggc ggacagatct
    25261 ggcgacaagg gccgcagtat ccggcgagcg gaacggcgcg tgccttgctc gaagacctgg
    25321 gtgcgcgtca gaacgtagcg ttgttgagcg gcgcgcgcat cgtgcaggcg ctatcggcac
    25381 acgccctgct cgccgagcgc gatggacgcg cgttgacgat cggttatcgc aaactgatcg
    25441 tcgcgaccgg tgcacgtgaa cgctttctgc cgtttcctgg ttggaccttg ccaggcgtca
    25501 cgggtgcggg ggggttgcag gcgctgatca agggcggcac gccagtgaga ggcgagcgca
    25561 tcgtgatcgc cggcagcggt ccgttactgt gggccgccgc cacgacggca cgtatgcagg
    25621 gcgcgcacgt cgtcgcgctg gtcgagcagg cttcctcggc gtcggtgggg cgctttgccc
    25681 gctcgctggt ccgaacgccg gccaaactgc tgcaggcggc gcgtatgcgt gccggcctct
    25741 ggaggacgcc gtacctgctc gacgcgtatg tcagcgaagc gaccggcgac gcaagtgtgc
    25801 gcgaggtgcg cgtgacccaa gcgggcagcg accggttggt tgcctgcgat cgcctcgcct
    25861 gtgcgtatgg tctactgccg aataccgcaa tcgcggccgc gctaggatgc cgcgtcgaca
    25921 cgcaagggca ggtggacacg attgcggtgg atgaatggca ggccacctcg gtgccggccg
    25981 tgttcgccgc gggcgaatgc acgggcgtgg ggggcatgga gttatcggcg gtggagggca
    26041 agattgccgc gctggcggcc gtcggtgagt ctgagcgcgc tcggccgttt tttgccgaac
    26101 gggcgcgcta tcggcgcttt gcccagcaca tgcacgaggc attcgaactg aatccgcgcc
    26161 tgcgcatgct cccgacaccg gacacgctgt tttgccgctg cgaggacgtg ccctatcggg
    26221 agatggcgca gcacacttca tggcgcgacg cgaaactgca cacgcgttgc ggcatgggtc
    26281 catgccaggg aaaagtatgc ggtgcggcag ccgcgttctg tttcggctgg cggcgcagcg
    26341 gcgagcagat gtcgcgtccg ccgttctctc cggtgcgcat tgaaacgctg ctgcaaatcg
    26401 ggtccgacgt gtaatttttc cttcaatcga cgctctgaca ttcgtgcccg acctggtgct
    26461 tcccgattcg agctgagttt cgactgtgca gatttcatct ctttcgtgcg cgaaaacccc
    26521 caagccccga cctggataaa acctgatact cattcacata ttcaacatgt gtcggcatga
    26581 ccgaccttta cgaaggagat gtgtac
//
LOCUS       DATASH010001108         6808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2961132,
            whole genome shotgun sequence.
ACCESSION   DATASH010001108 DATASH010000000
VERSION     DATASH010001108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6808
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2961132"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="VHV99_29450"
     CDS             complement(<1..896)
                     /locus_tag="VHV99_29450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HEX3639535.1"
                     /translation="MANSNSADIVVVGSGVAGGLVAHQMALAGASVILLEAGPRIPRW
                     QIVENFRNSPAKSDFATPYPSTPYAPHPEYAPANNYLIQKGDYPYNSQYVRLVGGTTW
                     HWAAAAWRLLPSDFQLHKLYGVGRDWPYPYETLEPWYSAAEMQLGVSGPDNSVDLGSP
                     RSKPYPMSQLPLSYMDQRFSNVLNAQGFKVVPEPVARNSRPYDARPTCCGNNNCMPIC
                     PIAAMYNGVVHAEKAEQAGAKLIAEAVVYRVEADNKGLITAVHYKDPNGNSTRVTGKL
                     FVLAANGIETPKLMLMSTTDKFP"
     gene            complement(1004..1570)
                     /locus_tag="VHV99_29455"
     CDS             complement(1004..1570)
                     /locus_tag="VHV99_29455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar dehydrogenase complex small subunit"
                     /protein_id="HEX3639536.1"
                     /translation="MRSSQAPSLQASRSFSFSASRRAWVLRACVTAAALAFAGAGRSL
                     SWIAPAFADAPTGGGLDAFLALSQRLTGRPGFDAVLGKRVYDALAKSDNQFPQNIATL
                     NTWLQGHGGVPSDTVTQALQADQPALAKSVSAIMRAWYLGLVGDMPHVDVVAYEKALM
                     FEPVKDVLTIPSYCRDVPFYWTQKPVHA"
     gene            2110..2772
                     /locus_tag="VHV99_29460"
     CDS             2110..2772
                     /locus_tag="VHV99_29460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HEX3639537.1"
                     /translation="MFTKYYPQVFRNRPRMLIGLAVGVVAALVVPEHTRVMVRVLVGW
                     DAAVWSYLVLIWVHMALADERGVREFARRDDENAGVVLAVICVATVASIAAIVLELAS
                     VKGTAGASTVWHSLLTGFTMIGAWFLIPTIFTMHYARLYYDPDAPETALLFPDDRLEP
                     NYWDFLYFSFTIAVASQTSDVVLRSRTIRRGALAQSILSFYFNVAVLGLCVNIAAGLL
                     GS"
     gene            3141..6581
                     /locus_tag="VHV99_29465"
     CDS             3141..6581
                     /locus_tag="VHV99_29465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltotransferase domain-containing protein"
                     /protein_id="HEX3639538.1"
                     /translation="MESPNGYAPRIYFFHSLLVGPLDAWPAQFAHAAGLGFDHALIGG
                     VFEPGQAGHGQVVGNHGRLHPVFETQQPATEAIRMLAQAARQHGLSLLVDLVIDRMAA
                     DGALYAEHAGWFHPFESEEARLDPRHVHREDNVAYANFNEAGSSAALVDWWTQQLLAL
                     AEAGVGGFRFDSPHRVPAAVWRQLGDAVRARHPELRFLAATPGLARGDLLSLQGAGFD
                     SVFSSVRWWDFRASWMVEEHAALARIAAPIAFPEAPYGTRLAADLSDAHDAAIVERAY
                     RRALFASASTGTGWMMPMGFEYGITEPMSQTRGDAAQFALACRSKRFDLSERIGHVNE
                     VVRNTKTLHANGELRPLSGPGAPAAVLLRADTPDLRHAGEAVLIAINPELGAPVRVDP
                     ARFLAGVPGNFTRFVPLDSPGHATPASLTPFTLAPGAARLFRAAADKPVRLAPPIDKA
                     NSKRSGRKTVLDAISAPRVAIESVTPSVDHGRFPAKRTVGERAEITAAIFAEGHDKIA
                     AAVVWRAADETAWHEVLMSPAQPAGLDIWKARIPLERLGRHEFTVIAWRDDFASLVEH
                     IQKKLKAGQTVELELDEAAHLFALVLAEVETAEGAVTEPLDHIVKVFTKADAETKLAL
                     ILAPTTAKAMIAARHRPFLSRDPIVYKIDADRTAAAFASWYEIFPRSMSDDESRHGTF
                     ADVIGKLPRIRDMGFDVLYFPPIHPIGQANRKGRNNTLNAQPGDVGSPYAIGAAEGGH
                     TAVHPELGTLDDFKQMLAAAHAHGLEIALDFAIQCSPDHPWLKAHPTWFAWRPDGTLR
                     YAENPPKKYQDIVNPDFYAHDAKPDLWLALRDVFLFWIEAGVHIFRVDNPHTKPLPFW
                     EWVIGDVRSRYPDTIFLAEAFTRPRMMNRLGKIGFSQSYTYFTWRESKRDFIEYMTEL
                     TQTSERDFYRPNFFVNTPDINPRHLQSSGRSGFLIRAALASTLAGLWGVYSGFELCEA
                     AALPNSEEYLNSEKYQLRAWDWNRPGNIVGEITPLNRIRRANPALQTHLGLSFLPAHN
                     DNILFFEKATEARDNVILVAINLDPFNEQGADIELSWDTFQHWNLHDHGALEVTDQMT
                     GARFEWHGRWQHIRLGSGQPFSIWRIAPLGGLPAERPDDADSDYHADAGNPTPTEGAT
                     "
     gene            6578..>6808
                     /locus_tag="VHV99_29470"
     CDS             6578..>6808
                     /locus_tag="VHV99_29470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639539.1"
                     /translation="MKRDDPAQPTSQAHTGAATGSAAKARAHRRGKPATLSDDPLWYK
                     DAIIYQVHIKSFFDANNDGVGDFPGLIAKLDYI"
BASE COUNT         1241 a         2246 c         2079 g         1242 t
ORIGIN      
        1 gggaatttgt cggtggtcga catcagcatc agcttcggcg tttcgatgcc gttcgccgcg
       61 agcacgaaca gtttgccggt cacgcgtgtg ctgttgccgt tcgggtcctt gtagtggacg
      121 gccgtgatga gccctttgtt atccgcttcg acgcggtaga ccaccgcttc ggcgatcagc
      181 ttcgcgccgg cctgctcggc cttttccgca tgcaccacgc cgttgtacat cgcggcaatc
      241 gggcagatcg gcatgcagtt gttattgccg cagcaggtag gccgcgcatc gtacggacgg
      301 ctgttacgcg cgaccggctc gggcaccacc ttgaaaccct gtgcgttgag cacgttgcta
      361 aagcgctgat ccatgtacga aagcggaagc tggctcatcg gatagggctt cgagcgcggc
      421 gaaccgagat cgaccgaatt gtccgggccc gacacgccga gttgcatttc agccgccgaa
      481 taccagggct ccagcgtttc atacggataa ggccagtcac gtcccacgcc atacagcttg
      541 tgcaactgga agtccgacgg caacagccgc cacgcagcgg ccgcccagtg ccacgtcgtg
      601 ccgccgacca gtcgcacgta ttgtgagttg tacggatagt cgcctttctg gatcagataa
      661 ttgttggccg gcgcgtactc gggatgcggc gcgtacggtg tagacggata gggtgttgcg
      721 aagtcggact tggccggtga gttgcggaaa ttctcgacga tctgccagcg cggaatgcgc
      781 ggtcccgctt cgagcagaat caccgatgcg ccggccagtg ccatctgatg cgcgaccagc
      841 ccacctgcca cgcccgaccc gaccacgacg atatcggcgg aatttgaatt tgccattgct
      901 gccctcttct actgccttat cgttgcttgc tgggtttcac atgccggcga ttcatcctga
      961 tcaaactcaa tcagcgatac ggcacgcacg agaggaacgt ccttcaggcg tgcaccggct
     1021 tctgtgtcca gtagaacggc acgtcgcggc aataggacgg aatggtcagc acgtccttga
     1081 ccggctcgaa catcagtgct ttttcataag cgaccacgtc cacatgcggc atgtcgccca
     1141 cgagaccgag ataccacgca cgcatgatcg cgctcacgga tttcgcgagc gccggctgat
     1201 cggcctgcaa tgcctgcgtg actgtatcgg acggaacgcc gccatggcct tgcagccacg
     1261 tgttgagcgt ggcgatgttt tgcgggaact ggttgtcgga tttggcgagg gcgtcgtaga
     1321 cacgtttgcc gagcacggcg tcgaagcccg ggcgaccggt gagacgctgc gagagcgcaa
     1381 ggaaggcatc gagtccacca ccggtgggcg catcggcgaa ggcaggggcg atccatgaga
     1441 gcgagcggcc cgcaccggca aacgcgagcg cggcggcggt gacgcatgcg cgcagcaccc
     1501 atgccctgcg tgatgccgag aatgaaaacg aacgcgatgc ctgaagcgaa ggcgcttgcg
     1561 aagaccgcag tgaagagttc gatgaagaac tccgcaaaga aggcagtgaa gacgaaattg
     1621 atgacgaaac cctgatcgaa tgcgtcactt cgtggtgcgc atctttattc tcgggaacat
     1681 ctgtcatggg ggctccagat ggtgagtgga tcgtgagtca ttctcaacgg acaacgcggc
     1741 catggcgtga tgccgctggc gcgttgcgtc gatttctcta ccccaaacct ggtctagcgc
     1801 cgcttaagcg gtcttgtatg actgcatgac cgttgcgaac cgcatgcagt acgtggtgca
     1861 gcgttacgtt ccgccgcatt acgttgtgct gatgacagcc gtgtgaaata gcttcactgc
     1921 gagcgccact gcaaactccg ccgcgcgctg cgcacggcga aaaacctgcc gcctgccaat
     1981 cgcctttcaa ctgaaagcgg cgcgcaaggc cgttgcgaaa gactatagcc tgtgactcac
     2041 ccattcgaca aatggtgcct gaagcgcgag tagaatccgc ggcacagacg cccctccaca
     2101 ctggatctca tgttcacgaa atattatccg caggtgtttc gcaatcggcc gcgcatgctg
     2161 atcggccttg ccgtcggtgt cgtggccgcg ctggtcgtgc ctgagcacac acgcgtcatg
     2221 gtgcgcgttc tggtcggctg ggacgccgcg gtatggagct atctcgtgct gatctgggtg
     2281 cacatggcgc ttgccgacga acgtggcgtg cgcgaattcg cgcggcgcga cgatgaaaac
     2341 gcgggtgtgg tgctggccgt gatctgcgtc gccacggtcg ccagcattgc cgcgatcgta
     2401 ctcgaacttg cctcggtgaa ggggacggcc ggcgcctcga cggtgtggca ctccctgttg
     2461 accggtttca cgatgatcgg cgcgtggttc ctgatcccga ccattttcac catgcattac
     2521 gcgcggctgt actacgaccc ggatgcaccg gagacggcgc tgctgtttcc cgacgacagg
     2581 ctcgagccga actactggga cttcctgtac ttttccttca ccatcgcggt ggcctcgcaa
     2641 acgtcggacg tggtgctgcg ctcccgcacg attcgccggg gagcgctcgc gcagtcgatc
     2701 ctgtcgttct acttcaacgt cgccgtgctc ggcctgtgcg tgaacatcgc ggcgggcctg
     2761 ctcggctcct agtcgtgtaa aacccggacg acacccaccc gttttacacg cgggccgtgc
     2821 gcggcggccg cgtgaactgg cctcaggccg ccttcctgtc atcctgccgg gtgcgcttcc
     2881 taccccttcc cctcgcccca aaacgcactg gcacaccgtt tgctgcgttc tgccaatagt
     2941 tctggccggc cccgtgccgt cgacgcctgg cgtcaaagca cctgctactg gccagcggcg
     3001 tgacgatctt tgcactacat tgaccagtac gcgccctcgc ttcacgcatc gcgcggtatc
     3061 cggctctgga agcccaatga gcgctgtgat cgcatgtcac ttgcatccac accgcagcag
     3121 gacgaatctg gaaacgaccg atggaatccc caaacgggta cgcgccgcgg atctactttt
     3181 ttcattcact tctcgttggg ccgctcgatg catggcctgc gcagttcgct cacgcagccg
     3241 gactgggctt cgatcacgcg ctgatcggcg gcgtcttcga gccgggccag gcaggtcacg
     3301 gacaggtggt cggcaaccac ggccggctgc atccggtgtt cgaaactcag caaccggcca
     3361 cggaggcaat ccgtatgctc gcgcaggccg cccgccagca tggcctaagc ttgctggtcg
     3421 atctggtgat cgaccggatg gccgccgatg gcgcgctgta cgccgaacat gccggctggt
     3481 tccacccatt cgagtctgaa gaggcgcggc tcgatccgcg ccatgtgcat cgcgaggaca
     3541 acgtcgcgta tgccaacttc aacgaagccg gcagcagcgc cgcactggtc gactggtgga
     3601 cgcagcaact gctggcgctg gccgaggccg gcgtgggcgg cttccgcttc gattcgccgc
     3661 atcgcgtgcc ggccgccgtt tggcggcagc tcggcgacgc ggttcgcgcc aggcaccccg
     3721 agttgcgttt cctcgccgcg acgcccggac tcgcccgtgg cgatctgctc agcctccagg
     3781 gcgcgggctt cgacagcgtg ttctcgtcgg tgcgctggtg ggactttcgc gccagctgga
     3841 tggtggagga gcatgccgcg ctcgcccgca tcgccgcccc catcgccttt cccgaagcgc
     3901 cttacggcac gcgcctcgcc gccgatctca gcgatgcgca cgacgccgcc atcgtcgagc
     3961 gggcctaccg gcgcgcgctg ttcgcgtcgg cctcgaccgg caccggctgg atgatgccga
     4021 tgggcttcga atacggcatc accgagccga tgtcgcaaac acgcggcgat gctgcgcagt
     4081 tcgccctcgc ctgccggtcg aagcgattcg acctctcaga gcggatcggc catgtcaacg
     4141 aggtggtgcg caatacaaaa acgctgcacg ccaatggcga attgcgcccg ctaagcggcc
     4201 ctggcgcacc tgccgccgtt ttgctgcgcg ccgacacgcc cgacctgcgc cacgccggcg
     4261 aagccgtcct gatcgccatc aatcccgaac tcggggcacc cgtgcgcgtc gatccggcgc
     4321 gcttcctcgc gggcgtgccg ggcaatttca cgcgcttcgt cccgctcgac tcgcccgggc
     4381 atgccacgcc cgccagcttg acgccgttca cgctcgcacc tggcgccgcg cgcctgttcc
     4441 gcgccgcagc cgacaagccg gtccgtctcg cgccgccgat cgacaaggcg aacagcaagc
     4501 gcagcggccg caagaccgtg ctcgacgcga tcagcgcgcc gcgcgtggcg atcgagagcg
     4561 tgacaccctc ggtcgatcac ggacgcttcc cagccaagcg cacggtaggc gagcgagctg
     4621 aaatcaccgc cgcgatcttc gccgaaggtc acgacaagat cgccgccgcc gtggtctggc
     4681 gcgccgccga cgaaaccgcc tggcacgaag tcctgatgag cccggcacaa ccggccggcc
     4741 tcgacatctg gaaggcgcgc attccgctgg agcgcctcgg ccgtcacgaa ttcacggtga
     4801 tcgcatggcg cgacgacttt gcgtcgctgg tcgagcatat ccagaagaag ctgaaggcgg
     4861 gccaaaccgt cgagctcgaa ctcgacgaag ccgcgcatct gttcgccctg gtgctcgccg
     4921 aagtggaaac ggcggaaggc gcggtgaccg aaccgctcga ccatatcgtc aaggtgttca
     4981 ccaaggccga cgccgaaacg aagctcgcat tgatcctcgc gccgaccacg gcgaaggcga
     5041 tgatcgccgc gcgccaccgg ccgttcctga gccgcgatcc gatcgtctac aagatcgacg
     5101 ccgaccggac cgccgcggct ttcgcaagct ggtacgagat cttcccgcgg tcgatgagcg
     5161 acgacgaatc gcgccacggc actttcgctg acgtcatcgg gaaactgccg cgcattcgcg
     5221 acatgggatt cgacgtgctg tacttcccgc cgatccaccc gatcggtcag gcgaaccgca
     5281 aaggccgcaa caacacgctg aacgcgcaac caggggatgt aggcagcccg tacgcgatcg
     5341 gcgcagccga aggcggtcac accgccgtgc atcccgaact gggcacgctc gacgacttca
     5401 agcagatgct cgccgccgcg cacgcgcacg gcctcgaaat cgcgctcgac ttcgcgatcc
     5461 agtgctcgcc ggatcacccg tggctaaagg cgcatccgac gtggtttgcc tggcgccccg
     5521 acggcacgct gcgttacgca gagaatccgc cgaaaaagta ccaggacatc gtcaatcccg
     5581 acttctatgc gcacgatgcc aaacccgatt tgtggctcgc cttgcgtgac gtattcctgt
     5641 tctggatcga agcgggcgtg cacattttcc gtgtggacaa tccgcacacc aagccgctgc
     5701 cgttctggga atgggtgatt ggcgacgtgc gatcgcgcta tcccgacacg atcttcctcg
     5761 ccgaagcctt cacccggccg cgcatgatga accgcctcgg caagatcggc ttctcgcagt
     5821 cgtacacgta tttcacgtgg cgcgaatcga agcgcgactt catcgagtac atgaccgagc
     5881 tcacgcaaac cagcgagcgc gatttctacc ggccgaactt cttcgtcaat acgccggaca
     5941 tcaatcctcg gcatttgcag tcgtccgggc gttcgggatt tctgattcgg gctgccctcg
     6001 cttccacgct cgccggcttg tggggcgtgt atagcggttt cgaattgtgc gaggccgccg
     6061 cgctgccgaa cagcgaggaa tatctcaact ccgagaagta ccaactgcgc gcgtgggact
     6121 ggaaccggcc cggcaacatc gtcggcgaga tcactccgtt gaaccggatt cgccgcgcga
     6181 acccggcgtt gcaaacgcat ctcggcctca gcttcctgcc cgcgcacaac gacaacattc
     6241 tgttcttcga gaaagcgact gaagcacgcg acaacgtcat cctggtggcc atcaatctcg
     6301 acccgttcaa tgaacagggc gcggacatcg aactctcgtg ggacactttc cagcactgga
     6361 atctgcacga ccatggcgcg ctggaagtga ccgatcagat gaccggtgcg cgtttcgaat
     6421 ggcacggccg ctggcagcac atccggctcg gttcgggtca gccgttttcg atctggcgca
     6481 tcgcgccgct cggcggcctg cccgccgaac gcccggatga tgccgacagc gactatcacg
     6541 cagacgctgg caacccaacc cctacggaag gtgcgacatg aagcgtgacg atccagccca
     6601 acccacgtcg caggctcata cgggcgcagc caccggcagc gctgccaagg cgcgtgcgca
     6661 tcggcgcggc aagcccgcga ccttgagcga cgatccgctc tggtacaaag acgcgatcat
     6721 ttatcaggtg cacatcaagt cgtttttcga cgcgaataac gacggcgtgg gcgattttcc
     6781 cggcctgatc gccaagctcg actacatt
//
LOCUS       DATASH010001109         5129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_388794,
            whole genome shotgun sequence.
ACCESSION   DATASH010001109 DATASH010000000
VERSION     DATASH010001109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5129
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388794"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2246
                     /gene="xdhB"
                     /locus_tag="VHV99_29475"
     CDS             <1..2246
                     /gene="xdhB"
                     /locus_tag="VHV99_29475"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdopterin binding
                     subunit"
                     /protein_id="HEX3639540.1"
                     /translation="DDIPTLAGTLHAALGLSQKAHAKIVSMSLDKVRATPGVVAIFTA
                     DDIPGANDVGPIIHGDDPILADGLVQYVGQPIFIVVATSHEIARLAARRAEVVYEELP
                     AILTAQQARAANQHVLPPMKLARGEAGTKIARAAHREAGEMLLGGQEQFYLEGQISYA
                     VPKDDDGMHVYCSTQHPTEMQHMVAHALGVASHNVLIECRRMGGGFGGKESQSGLFAC
                     CAALAAWKLLCPVKLRPDRDDDMMVTGKRHDFHYTYEVGYDDKGVIDGVAVDMTSRCG
                     FSADLSGPVMTRALCHFDNAYWLSDVTIDGFCGKTNTQSNTAFRGFGGPQGAFAIEYI
                     MDNVARSVGEDSLDVRRRNLYGKTERNQTPYGQVVEDNVIHELIDELEATSEYRARRA
                     AINEFNANNEVLKKGLALTPVKFGIAFNVTHFNQAGALVHIYTDGSVLVNHGGTEMGQ
                     GLNTKVAQVVAHELGVGFNRIRVTATDTSKIANTSATAASTGSDLNGKAAQDAARQLR
                     ERLSAFAAERFGAGQVAASEVRFVHDRVVVGEVIVPFEEVIAKAYIARVQLWSDGFYA
                     TPKLYWDQSKLQGRPFYYYSYGAAVSEVVIDTLTGEMRVLRADALHDVGASLNPALDV
                     GQVEGAFIQGMGWLTTEELWWNAGGKLMTHAPSTYKIPTVNDTPPDFRVRLFKNRNAE
                     DSIHRSKATGEPPLLLPFSVFFAVRDAVSAVGNHKVNPPLNAPATSEEILKAVGAVRA
                     ATAAARQ"
     gene            2333..2869
                     /gene="xdhC"
                     /locus_tag="VHV99_29480"
     CDS             2333..2869
                     /gene="xdhC"
                     /locus_tag="VHV99_29480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory protein XdhC"
                     /protein_id="HEX3639541.1"
                     /translation="MNAFSSIQIGRRSAVPAPRPAPMHIVLFGAGHVGHALVTLLGSL
                     PCVVQWVDERDELFPDETPANVQIEATDTPDAIVDTAPPGAYFLVMTHNHALDFSLAA
                     RIMRRRDFTYFGMIGSKTKRVKFERRLRDRGVDLDRLVEMTCPIGVTGIVDKAPPAIA
                     VAVCAELLQVRAHRNGNQ"
     gene            complement(2928..3911)
                     /locus_tag="VHV99_29485"
     CDS             complement(2928..3911)
                     /locus_tag="VHV99_29485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX3639542.1"
                     /translation="MDDDSAASLDIWLVRVLRTLLVERSVTQTALRLNQTQPAISTAL
                     RKLRETLNDPILVRGKSGMVPTEYGESLLGSAQRVLREVDFVATPHGDFDPGRSRRTF
                     RVAAPDYLNDFFMPTVIAQFREAAPHARLEIDSLGPMLDHSAALEAGELDLVIGNWPK
                     PDPRFERSDLFSDTVVCMMRADHPLTRAPMTREAYLAAPHLAPTPYSGTRGGAVDIGF
                     ARARAERRIVATLPYFGLVPQTLLQSDLIFTTTRRFAAHYASMLPLAVVEVPIPFPRI
                     KCYQLWHPQPDRPSDVGWLRTLMSQVSDELVARKVRGAKRAPKREKSAAAG"
     gene            complement(4135..4779)
                     /locus_tag="VHV99_29490"
     CDS             complement(4135..4779)
                     /locus_tag="VHV99_29490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HEX3639543.1"
                     /translation="MTVGIDAKQPLWFYDFVSPFTYLLLEQHDKWPGFDFAFTPVVLN
                     DLYHHWGQRPAYSVPAKRTFLYRHALFRAEQLGIPFKMPPAHPFESMKPLLLATAAKG
                     NISFVREIFRFIWREGRDPSSDAAFAELCARVGMPDGPELIRNQEVKAQLQRNTADAI
                     GLGVYGVPTFRLNDQLFWGEDALPMVLYCARTPNWLDSGEVKRISSLPSGLADV"
     gene            4900..>5129
                     /locus_tag="VHV99_29495"
     CDS             4900..>5129
                     /locus_tag="VHV99_29495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639544.1"
                     /translation="MDKLAVVLTALVSGFCRIGRRGLRVFVVWFGLRPFVWSVLRRGA
                     SGDVFGLFRLVLGALAGVREPPAHEADMLAQY"
BASE COUNT          943 a         1585 c         1672 g          929 t
ORIGIN      
        1 ccgacgacat ccccacgctc gccggcacgc tgcatgccgc gctcggtttg tcgcagaaag
       61 cgcacgcgaa gatcgtctcg atgtcgctcg ataaagtgcg tgccacgccc ggcgtggtcg
      121 cgatcttcac cgcggacgat atccccggcg ccaacgatgt gggcccgatc atccacggcg
      181 acgatccgat tctcgccgac ggcctggtgc aatacgtcgg ccagccgatc ttcatcgtgg
      241 tcgcgacctc gcatgaaatc gcgcgcctcg ctgcgcgccg cgccgaagtc gtctatgaag
      301 aattgccggc cattctcacc gcgcaacagg cccgcgccgc gaaccagcac gtcttgccgc
      361 cgatgaaact cgcgcgcggc gaagccggca cgaagatcgc ccgcgcggcg catcgcgaag
      421 ccggcgagat gctgctcggc ggccaggaac agttctatct ggaaggccag atttcgtacg
      481 cggtgccgaa ggacgacgac ggcatgcacg tctattgttc gactcagcat ccgaccgaaa
      541 tgcagcacat ggtcgcgcac gcgttgggtg tggcatcgca taacgtgctg atcgaatgcc
      601 gccggatggg cggcggtttc ggcggcaagg aatcgcaatc gggtctgttc gcgtgctgcg
      661 cggcgctcgc tgcatggaag ctgctgtgcc cggtcaagct gcgtccggac cgcgacgacg
      721 acatgatggt caccggcaag cgccacgatt tccactacac gtacgaagtg ggctacgacg
      781 acaagggcgt gatcgacggc gtcgccgtcg acatgacctc gcgctgcggt ttctccgccg
      841 acctgtccgg tccggtgatg acgcgcgcgc tgtgccactt cgacaacgct tactggctct
      901 ccgacgtgac gatcgacggt ttctgcggca agaccaacac acagtcgaat accgcgttcc
      961 gcggcttcgg cggtccgcaa ggcgcgttcg cgatcgagta catcatggac aacgtcgcgc
     1021 gttcggtggg cgaggattcg ctcgacgtgc gccgtcgcaa tctgtacggc aagactgagc
     1081 gcaatcagac gccgtatggt caggtggtcg aagacaacgt gattcacgag ttgatcgacg
     1141 aactcgaagc gaccagcgaa taccgcgcgc gtcgtgcggc catcaacgag ttcaacgcga
     1201 acaacgaagt gctgaagaag ggcctggcgc tcacgccggt caagttcggc atcgcgttca
     1261 acgtgaccca cttcaatcag gccggcgcac tggtgcatat ctacaccgac ggttcggtgc
     1321 tggtgaacca cggcggcacc gaaatgggcc aggggttgaa caccaaggtc gcacaggtgg
     1381 tcgcgcatga actgggcgtc ggctttaacc gcatccgcgt gacggcgacc gatacgagca
     1441 agatcgccaa tacgtcggcg accgctgcat cgacgggttc ggacctgaac ggcaaggccg
     1501 cgcaagatgc cgcgcggcag ttgcgcgaac gtctgtcggc ttttgccgcc gagcgtttcg
     1561 gcgcggggca ggtggccgcc tcggaagtgc gtttcgtgca cgaccgcgtg gtggtcggcg
     1621 aagtaatcgt gccgttcgaa gaagtgatcg cgaaggccta catcgcgcgt gttcagttgt
     1681 ggtccgacgg cttctatgca acgccaaagc tctactggga tcaatcgaag ctgcaaggcc
     1741 gcccgttcta ctactattcg tacggcgccg ccgtgtccga agtggtgatc gatacgctga
     1801 ccggcgaaat gcgcgtgctg cgcgcggacg ctttgcacga cgtgggcgca tcgttgaacc
     1861 cggcgctcga cgtcggccag gtggaaggcg cgttcattca gggcatgggc tggcttacca
     1921 ccgaagaact gtggtggaac gctggtggca agctgatgac gcacgcgccg tccacctaca
     1981 agattccgac cgtcaacgat acgccgcccg atttccgtgt gcgcctgttc aagaatcgca
     2041 acgcggagga cagcatccat cgttcgaagg cgaccggcga gccgccgctg ctgctgccgt
     2101 tctcggtgtt cttcgccgtg cgcgatgcgg tgtcggccgt gggcaaccac aaggtcaatc
     2161 cgccgctgaa cgcgcccgcg accagcgagg aaatcctcaa ggcagtcggc gcggtgcggg
     2221 cagcaactgc agcggcccgg cagtaggcat tgccagatgg caattgccga ttggcatttg
     2281 tctccacgca gagcaattga tgcaactatc gaaaccaact ggaatgatcg cgatgaacgc
     2341 attttcttcc attcagatcg gccggcgcag cgccgtaccc gcgccgcgtc cggcgccgat
     2401 gcatatcgtg ctgttcggcg cggggcacgt cggccatgcg ctcgtcacgc tgctcggcag
     2461 cttgccgtgc gtcgtccagt gggtcgatga gcgcgacgaa ctgttccccg acgaaacccc
     2521 cgccaacgta cagatcgaag cgaccgacac acccgatgcg atcgtcgaca cggcgccccc
     2581 cggcgcgtat tttctcgtga tgacgcacaa ccacgcgctc gatttttcgc tcgccgcgcg
     2641 catcatgcgg cggcgcgatt tcacctactt cggcatgatc ggctcgaaga ccaagcgcgt
     2701 gaaattcgag cgtcgtttgc gggatcgagg cgtggatctg gatcggctgg tggagatgac
     2761 ctgcccaatc ggcgtgaccg gtatcgtcga caaggcaccg ccggcgattg ccgttgcggt
     2821 gtgcgcggaa ttgctgcaag ttcgcgcaca ccgcaacggc aatcagtgac gcagaagctc
     2881 tacgcgagcg tgtaagcggg ccgagcaaac gggacttaac ccgaccttca acccgccgcg
     2941 gccgactttt cccttttcgg cgctcgcttc gcgccccgca ctttccgcgc gaccaattca
     3001 tccgacactt gcgacatcaa cgtgcgcaac cagccgacgt cgctcggccg gtccggctgc
     3061 ggatgccata actgatagca cttgatgcgc ggaaacggaa tcggcacttc gaccaccgcg
     3121 agcggcagca tgctcgcata gtgcgccgcg aaccggcgtg tggtcgtgaa gatcagatcc
     3181 gattgcagca acgtttgcgg cacgaggccg aagtagggca gcgtggcgac gatgcgccgc
     3241 tcggcgcggg cccgcgcaaa gccgatgtcg accgcgccgc cgcgcgtgcc gctatatggc
     3301 gtcggcgcca gatgcggggc ggcgaggtac gcttcgcgcg tcatcggcgc gcgtgtcagc
     3361 ggatgatcgg cgcgcatcat gcacaccacc gtatcggaga acagatcgct gcgttcgaaa
     3421 cgcggatcgg gctttggcca gttgccgatc accagatcga gctcgcccgc ctcgagcgcg
     3481 gccgaatggt cgagcatcgg gccgagcgaa tcgatctcga ggcgcgcatg cggcgccgct
     3541 tcgcggaact gcgcgatcac ggtgggcatg aagaagtcgt tcagataatc cggcgcggcc
     3601 acgcgaaagg tgcggcgcga gcggcctgga tcgaagtcgc cgtgcggcgt tgccacgaaa
     3661 tcgacttcgc gcagcacacg ttgcgccgag cctagcaatg attcgccata ttcagtcggc
     3721 accatgcccg acttgccgcg cacgaggatc gggtcgttca gcgtctcgcg caatttgcgc
     3781 agcgcggtgc tgatcgcggg ctgggtctga ttcagacgca gtgcggtttg tgtgacgctg
     3841 cgctcgacaa gcagcgtgcg cagcacgcgc acgagccaga tatcgaggga ggcggcggag
     3901 tcgtcgtcca tggtttggcg aggcgatgca agcaggtcgg atccggagga agcctcgtac
     3961 ggagccgccc gcgagccaga tcgcgagagc gaggcgcaaa ggttataacc ttaggcccgt
     4021 ttgcggtgcg gcggcatagc gggcgcagta tggccggcat caggacggcg cgtttgacgc
     4081 aatctcgtct gaaaccgcgt gcctggttgc acaagtatta cggtttgcct tcgctcaaac
     4141 gtccgcaagg ccggaaggca gcgaactgat ccgcttcact tcccctgagt cgagccagtt
     4201 cggtgtgcgt gcgcaataca gcaccatcgg caatgcgtct tcgccccaga acaattgatc
     4261 gttcagacgg aaggtgggca cgccgtagac gcccaggccg attgcatccg cggtgttgcg
     4321 ctgcaattgc gctttcactt cttggttcct gatgagttcc ggaccgtccg gcatgccgac
     4381 gcgcgcgcac aactcggcga acgccgcatc gctcgacgga tcgcggcctt cacgccagat
     4441 gaaacggaaa atctcgcgca cgaagctgat gttgcccttc gccgcggtgg cgaggaggag
     4501 cggcttcatg gactcgaacg gatgcgccgg cggcatcttg aacggaatgc cgagttgctc
     4561 cgcgcgaaac agcgcgtggc gatacaggaa ggtgcgcttc gcgggcacgc tgtaagcggg
     4621 ccgctggccc cagtggtgat acaggtcgtt cagcacgacc ggagtgaacg cgaagtcgaa
     4681 gcccggccat ttgtcgtgtt gctcgagcaa caggtacgtg aacggcgaga caaaatcgta
     4741 aaaccacagc ggttgcttgg cgtcgatgcc aacggtcata aatgtctccc ggattgcctg
     4801 tgtccgacgg tgcgtctgct atcccgtcaa aattgtgatg atcttacgcg gtgcgggcat
     4861 ggcttgcacc cgtcaaatgg tttggtgtcg gtgcggccga tggataagct tgccgtggtg
     4921 ctgacggcgc ttgtatcagg tttttgccgg atcggtcgca ggggcttgcg tgttttcgtc
     4981 gtgtggttcg gtctgcggcc cttcgtgtgg tccgtgctgc ggcgcggggc gagcggcgac
     5041 gtgttcggct tgttccggct cgtcctgggc gctctcgcgg gcgttcgtga gccgccggcg
     5101 cacgaagccg atatgctcgc gcaatacat
//
LOCUS       DATASH010001110         5018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6519759,
            whole genome shotgun sequence.
ACCESSION   DATASH010001110 DATASH010000000
VERSION     DATASH010001110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5018
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6519759"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /gene="flgJ"
                     /locus_tag="VHV99_29500"
     CDS             <1..922
                     /gene="flgJ"
                     /locus_tag="VHV99_29500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HEX3639545.1"
                     /translation="GKLSAQAKASPQAGMKMAAQQFDAVFTQMMLKSMRDATPQDGPF
                     DSHDTATFTSMMDQQLSQQLSQKGIGVADAMLKQMMRNQGMQVGGANGTSGGLAGMAN
                     ALGGGSGGDEGQTAALNALAKAYGNAQANGQLAMGRGYSANSSLTPPIKGDGSSPKVD
                     AFVDKLAQPAQAASAATGIPARFIIGQAALESGWGKSEIRKSDGSTSHNVFGIKATGD
                     WTGKTVSTVTTEYVNGKPQRTVEKFRAYDSYQEAMTDYASLLKGNPRYAQVINSARDV
                     NGFANGMQRAGYATDPHYAKKLMSIMQKMG"
     gene            1356..2132
                     /locus_tag="VHV99_29505"
     CDS             1356..2132
                     /locus_tag="VHV99_29505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar regulator YcgR PilZN domain-containing
                     protein"
                     /protein_id="HEX3639546.1"
                     /translation="MDTNQSHGQTDALGEPHATLDESAPDFGRRNPLEIGVQLRNLVN
                     RGDFLTVEYAGGQLVTRLLDVDVRGRTFTFDWGALSAQNKGLLGASRCQFHASPDGVR
                     VEFATAAPRETRYEGLPAFEANFPEVLFYVQRREYFRVDAPILDPYMCTGRFSEGDTF
                     RFEVHDLSLGGVGMRTTDERVAELPMGTRLEDCELLFGSFGRLSLDLQLVSHRSTSLP
                     NGTQRYQLGFRFVTLPGSAENTLQRLITQLEMKRRSLVRP"
     gene            2351..4345
                     /gene="flgK"
                     /locus_tag="VHV99_29510"
     CDS             2351..4345
                     /gene="flgK"
                     /locus_tag="VHV99_29510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HEX3639547.1"
                     /translation="MSDNLISIGLSGLNAAQWGLTTTGQNISNASTPGYTIETPVYAD
                     SSGTYTGSGYLPQGVSTVTVSRQYSQYLTTELNNAQSSGSSLSSYNTMITQLNNLIGS
                     PTAGIASAITGYFTGLQNVANSASTLATRQTAMSGAQTLVNQINSAGQQYDSLRQSVN
                     TQLTSTVSQINSYTQQIAQLNGQISAASTQGQPPNQLMDERDQAVSNLSQLIGVNVVN
                     SNGSYSVFMNNGQPLVSAGNSYNLGTAVSPGDTSEVSVQYLGQAGANPAAAPQTLPDS
                     NVTGGSLGGLVAFRSQTLDPAEAQLGAIAVSFSAQVNAQNALGITLNGTQGGALFSVG
                     APTVYANTQNTGNASLSVSFANAAQPTTGDYTLSYNGTAYTLTDNSSGAVVGSATSLS
                     QPIDGLNFSTTGTMNAGDSFTVEPTRGALNSFATATTDPSAIAAASPVLGSAAVSNTG
                     TGTISSGTVTAGYTMPNATTTLSYNGTTIPGILSGFPAGSTVTVTAGTPPTTTTYDTT
                     TTPVVPYSATTGATLTINNTTAGSMNGVTFTISGAPATGDSFKIGPNTGATNDGSNAL
                     ALSNLSNAKSMSGGTLTLVGSYANYVNQVGNQTNQIQTSSTAQTTLVSQITSAQQSVS
                     GVNLNEEAANLLQYQEAYQANSKVIQTAQTLFQTLIGIFQ"
     gene            4356..>5018
                     /gene="flgL"
                     /locus_tag="VHV99_29515"
     CDS             4356..>5018
                     /gene="flgL"
                     /locus_tag="VHV99_29515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HEX3639548.1"
                     /translation="MRISSSEYFNMNVSTMSNEQAQLSQLYAEISSGVSLNTPSDNPL
                     GAAQAVQLSSTATSLSQYTTNQTTALASLQQEDSTLGSVNTVLASIHTLILQAGDASL
                     NNSNRGAIATQLQGLSSQLMTLANTTDPQGNYLFAGFQSTAQPYTTNSAGVVTYSGDT
                     GVRTVQVTGSNSVQTADNGVSIFGSVAPIGTSAVPAAAATNTGSGVIGSVSLTNPINP
                     ANA"
BASE COUNT         1061 a         1585 c         1505 g          867 t
ORIGIN      
        1 cggcaaattg agcgcgcagg ccaaggcttc gccgcaagcc ggcatgaaga tggccgcgca
       61 gcagttcgac gcggtgttca cgcagatgat gctgaagagc atgcgcgatg cgacgccgca
      121 ggatggtccg ttcgattcgc acgacaccgc caccttcacg tcgatgatgg atcagcagtt
      181 gtcgcaacag ctgtcgcaga aaggcatcgg cgtggctgac gcgatgctca agcagatgat
      241 gcgcaatcag ggcatgcagg tgggcggcgc gaatggcaca agcggtggcc tcgccggcat
      301 ggccaatgcg ttgggtggcg gcagcggcgg cgacgaaggc cagactgcgg cgctgaacgc
      361 gcttgcgaag gcctacggca atgcacaggc caatggtcag ctcgcgatgg gccgaggcta
      421 ctcggccaac agctcactga cgccgccgat caaaggcgac ggcagctcgc cgaaggtcga
      481 tgctttcgtc gacaagctgg cccagccggc gcaagccgcc agcgcggcga ccggcattcc
      541 ggcgcgcttc atcatcggcc aggccgcgct cgaatcgggc tggggaaaaa gcgaaatcag
      601 gaagtcggac ggttcgacca gccacaacgt attcggcatc aaggcgaccg gagactggac
      661 cggcaagacc gtctcgacgg tcacgaccga gtatgtgaac ggcaagccgc aacgcacggt
      721 tgaaaaattc cgcgcgtacg actcgtatca ggaagcgatg accgattacg ccagcctgct
      781 caagggcaac ccgcgttatg cgcaagtgat caattccgcg cgtgacgtga atggctttgc
      841 caatggcatg cagcgcgcgg gttacgcgac cgacccgcat tacgcgaaaa agctgatgtc
      901 catcatgcag aagatgggct gagtccgttt tatttcgacg tgtggccgca gccggccacg
      961 gtgtcgatca gcaattgcag tttgaatcac ccgaataaaa gcatgcacgc gacggggcca
     1021 ggtgcataac aagacggacg aggccggctg cctgtgggcg gccggtccac ccgcataatt
     1081 tgcgtgatcg tccgctggtt ttgatgcagc ggattgcgct gtcggcatat cgatgacgta
     1141 ccgacggggt ctcggcgttt taacgtttgc ggaaatattt catgagcggc ccctaaattt
     1201 tgcttgaatg ctgccgctaa tcagttagac ccgataccgg aaatacgttg taatgtccgg
     1261 ttgaatgcgc gtgccgcggc ttcgatggag accgcgcaaa agccccggca ggagccttgg
     1321 cacgtgcccg caagaacatg cataccggca agcccatgga tacaaaccag tcgcacggcc
     1381 aaaccgacgc actcggtgag ccgcacgcca cgctagacga aagtgcaccc gatttcggcc
     1441 gtcgcaatcc gctggagatc ggcgttcagt tacgcaacct cgtcaatcgg ggcgatttcc
     1501 tcaccgttga atacgcgggc ggtcagctcg taacgcgcct gctcgatgtc gatgtgcgcg
     1561 gacgcacctt cactttcgat tggggcgccc tctccgccca gaacaaaggc ttactgggcg
     1621 catcgcgctg ccagttccat gcgtcgccgg acggcgtgcg cgtcgagttc gccaccgccg
     1681 cgccacgcga aacccgctac gaaggattgc ccgcattcga ggcgaacttc cccgaggtgt
     1741 tgttctacgt gcagcgccgc gagtatttcc gcgtcgatgc gccgattctc gatccgtata
     1801 tgtgcaccgg ccgtttttcc gagggcgaca cgttccgttt cgaagtgcac gatctgtcgc
     1861 tcggcggcgt cggcatgcgc accacggatg aacgcgtggc cgaattgccg atggggacga
     1921 ggcttgagga ttgcgagctg ttgttcggtt cgtttggccg gctttcgctc gatctgcaac
     1981 tggtgtcgca ccgctcaacc tcgctgccga acggcacgca gcgctatcaa ctcgggttcc
     2041 gttttgtgac cttgccgggc agtgctgaaa acacgctgca acggctgatt acgcagctcg
     2101 aaatgaagcg ccgctcgctg gtgcgcccat aaaaacgctg gatgggcggt gtctgggcat
     2161 ggctcggatg ccgcccgctt tgcgcctgaa tgcggagccg gcaaaagccc gctaacacag
     2221 gcctcgcaaa cgctgcaaca aacgtcgaac acgcatcgga tcgccctcaa tttttcctcg
     2281 tgggggccgt taaacagaat gttcaagtaa cgcggcggca tgcggtcggt gtcgcccttc
     2341 aggatgacgc atgtcggaca atctcatcag tatcggcctc agtggactga acgcagccca
     2401 atggggactc acgacgaccg gtcagaatat cagcaacgcg tccacgccgg gctacacgat
     2461 cgaaacgccg gtctacgccg acagcagtgg cacgtacacg ggttccggct atctgccgca
     2521 gggcgtctcg acggtcaccg tttcgcgcca gtacagccag tatctgacca ctgagctgaa
     2581 caacgcgcaa agctcgggca gctcgctgtc gtcgtacaac acgatgatca cgcaactgaa
     2641 caatctgatc ggcagcccga ccgccggtat tgccagtgcg atcaccggct atttcacggg
     2701 tttgcagaac gtcgcgaaca gcgcctccac gctggccacc cgccagaccg cgatgagcgg
     2761 cgcgcagacg ctcgtcaacc agatcaactc cgcgggacag caatacgatt cgttgcgtca
     2821 gagcgtcaac actcagctca ccagcaccgt ttcgcagatc aatagctaca cgcagcagat
     2881 cgcgcagttg aacggacaaa tctcagcggc cagcacgcaa ggtcaaccgc cgaaccaact
     2941 gatggatgag cgcgatcaag ccgtgtcgaa tctgtcgcag ctgatcggcg tgaacgtcgt
     3001 caatagcaac ggcagctaca gcgtgttcat gaacaatggc cagccgcttg tgtccgctgg
     3061 caacagctac aacctgggca cggcggtttc gccgggcgac accagtgagg tctcggtgca
     3121 gtacctgggg caagctggcg caaacccggc cgccgcgccg cagactctgc ccgacagcaa
     3181 cgtgacgggt ggctcgctcg gcggcctcgt ggcgttccgt agtcagacgc tcgacccggc
     3241 cgaagcgcaa ctcggcgcga ttgccgtgag cttctccgcg caggtcaacg cccagaacgc
     3301 actcggcatt acgctcaacg gcacccaggg cggcgcactg ttttcggtgg gcgctccgac
     3361 cgtctacgcg aacacgcaga acaccggcaa tgcctcgttg agcgtgtctt tcgcgaatgc
     3421 tgcgcagccg accaccggcg actacacgct ctcctataac ggcacggcct atacgttgac
     3481 cgataattcg agcggcgccg tggtgggctc ggcaaccagc ttgagccaac cgatcgacgg
     3541 tctgaatttc tcgaccaccg gcacgatgaa cgcaggcgat tcgtttacgg tcgagccgac
     3601 tcgcggcgcg ctgaacagtt tcgccaccgc gactacggat ccttcggcga ttgcggccgc
     3661 ctcgccggtg ctcggctcgg ccgcggtgag caacaccggc accggtacga tttcgtcggg
     3721 cacagtgacg gcaggctaca ccatgcccaa cgcgacgacc acgctgtcgt acaacggcac
     3781 gacaattccc ggcatcttgt ccggcttccc ggccggctcc acggtgacgg tgacggctgg
     3841 cacgccgccg accaccacca cctacgacac cacgaccacg cctgtcgtgc cgtattcggc
     3901 aaccaccggc gcgacgctga cgatcaacaa cacgacagcc ggcagcatga atggcgtgac
     3961 cttcacgatc agcggcgcac ctgccaccgg cgacagcttc aaaatcggcc cgaacaccgg
     4021 cgcgaccaac gacggcagta acgcacttgc gttgtcgaat ctgtccaacg cgaagtcgat
     4081 gtcgggcggc acgctcacgc tggtcggctc gtatgcgaac tacgtcaacc aggtgggcaa
     4141 ccagaccaac cagattcaga cgtcgagtac ggcccagacc acgctagtgt cgcagatcac
     4201 cagtgcgcag cagtcggtct ccggcgtgaa cctgaatgag gaagccgcta atctgctcca
     4261 gtatcaggag gcctatcagg ccaacagtaa agtcattcag accgcgcaga ctctgttcca
     4321 gacgttgatc ggcattttcc agtgaggcgt tgaacatgcg tatctccagt tcagagtatt
     4381 tcaacatgaa cgtgtcgaca atgagcaatg agcaagctca gttgtcgcag ctgtatgccg
     4441 agatttcgag cggcgtgagc ctcaacacgc cgtcggacaa tccgctcggc gcggcgcagg
     4501 cggtgcagtt gagttcgact gcgacaagct tgtcgcaata cacgactaac cagacgaccg
     4561 cgctcgcgtc gctgcaacag gaagactcca cgctgggcag cgtcaacacc gtgttggcga
     4621 gcattcacac gctgatactg caagccggcg acgcttcgct gaacaacagc aaccgcggcg
     4681 caattgccac acagttgcag ggcttgagca gccagttgat gacgctggcc aacacgaccg
     4741 acccgcaggg caactacctg ttcgccggtt ttcaaagcac cgcgcagccg tacaccacca
     4801 attcggccgg cgtcgtgact tattcaggtg acaccggcgt gcgcaccgtg caggtcaccg
     4861 gctcgaacag tgtccagacg gccgacaacg gtgtctcgat cttcggcagc gtcgcaccga
     4921 tcggcacgag cgcggtgccg gccgcggcgg cgacgaatac gggcagcggc gtgataggca
     4981 gcgtcagctt gaccaatcca atcaatccgg ccaatgcg
//
LOCUS       DATASH010001111         6473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2171629,
            whole genome shotgun sequence.
ACCESSION   DATASH010001111 DATASH010000000
VERSION     DATASH010001111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6473
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2171629"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..133
                     /locus_tag="VHV99_29520"
     CDS             <1..133
                     /locus_tag="VHV99_29520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012328183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HEX3639549.1"
                     /translation="GDYRYTVRHDEERIVFPNATVKPGLRAGLLVVETTEETLSKLV"
     gene            281..1087
                     /locus_tag="VHV99_29525"
     CDS             281..1087
                     /locus_tag="VHV99_29525"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006025859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3639550.1"
                     /translation="MKMNWRNMAALALFATATASLTLTAGTASAADIKEVRFGVEASY
                     APFESKSPAGELQGFDIDVGNAVCAKLKAKCVWVENSFDGLIPALAARKFNAINSDMT
                     ITEQRRQAIDFTDPIYTIPNQMIAKKGSGLLPTAASLKGKHVGVLQGTIQEAYAKARW
                     APAGVDVVPYQTQDQIYADLASGRLDAAFQDAEAASKGFLKKPQGAGFEFAGPAVTDE
                     KLLGAGVGFGIRKGDKALKDALNQALKELKADGTIDRFAAKYFDVKVVLK"
     gene            complement(1153..1869)
                     /locus_tag="VHV99_29530"
     CDS             complement(1153..1869)
                     /locus_tag="VHV99_29530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3639551.1"
                     /translation="MNSVIGKIAVVTGAGGGVGKQIALALARAGAVVGVADLNPQGVK
                     EVVQQIRKSGGTAVCMAMDVTNEDAVNEGIDRVVAQFGALDVLVANAGVQILNPLESN
                     SFSDWRKMQATHVDGAFLTTRAALRHMYRDDRGGVVIYIGSVDAQDTSPLMCAYAAAK
                     HALLGIARVLAKEGAPHNVHAYLVCHGAGDDDVVRHVAPRGGADTSFATAHEVAQTVL
                     HLASSSTRPLATHSPLDCVK"
     gene            complement(1963..2694)
                     /locus_tag="VHV99_29535"
                     /pseudo
     CDS             complement(1963..2694)
                     /locus_tag="VHV99_29535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487770.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase"
     gene            complement(3031..3768)
                     /locus_tag="VHV99_29540"
     CDS             complement(3031..3768)
                     /locus_tag="VHV99_29540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase"
                     /protein_id="HEX3639552.1"
                     /translation="MDVKSVLSNAFAMPITSPAFPMGPYRFIDREFLIITYRTDPDKL
                     RAVVPEPLQIGEPLVHYEFIRMPDSTGFGDYTESGQVIPVSYNGVAGGYTLAMYLDDH
                     PPIAGGRELWGFPKKLAKPVLEVHTDTLVGTLDYGPVRIATGTMGYKHRQLDLAQQQK
                     RLETPNFLLKVIPHVDGTPRICELVRYYLQDITLKGAWTGPAALQLAPHALAPVAELP
                     VLEIVEARHLVADLTLGLGEVVFDYLK"
     gene            4044..5441
                     /locus_tag="VHV99_29545"
     CDS             4044..5441
                     /locus_tag="VHV99_29545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018432300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX3639553.1"
                     /translation="MTTIAIENPGQTDATADAQLNASFARQPILNRDGMLCGYEIKVR
                     APALPAGVEAGAGAAAALAGDSATDADALPGRAPEATQLLVRAIVRGLIHSNVRGALT
                     GHPAYVDVSREMLFDDSILRLPAARFMFELSPSMEVDDALVARIVQLHARRYRFALDE
                     VTQPNETFAKLLPYAEVVKIDFMHAPRALLPKLTSVLKAAGKLLVALGIDAQDDFEMA
                     HELGFDRFQGYFFARPQTSMMRRVSAPRHALLNLLQLLAGEPTVAQLEAELKLNPVLV
                     MHLMKLANSSGLAVGYKVSTLREAINATGTNRIARWTQLLLYADGRKVALEDDPLLQL
                     AATRARFMELAIERLPEAGRDEADAAFLTGVFSFVDAVFGGSLESTLNVLTLSRPIQA
                     GILHRQGVLGLLLTVVEALERGAWDEVDSLCARLQPLTAEDVAALGLAAGAWAGVADR
                     SAEGLEMIEDWSSED"
     gene            complement(5550..>6473)
                     /locus_tag="VHV99_29550"
     CDS             complement(5550..>6473)
                     /locus_tag="VHV99_29550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018418508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX3639554.1"
                     /translation="ACVLVLGVTFDEIGAEAAVRWRLPDMIRAGMGEFDPLDVEQPRQ
                     VQWLRAITNYSTAVADVLTQQNMPDWQREERIAGLAQAYSHALNTDPEVLHQMSIALA
                     REEGGDGVMAEIVELRANADAIAREALNPEARIAAGVGDLRGLPDGSALAPALALAAE
                     TVLAALGFARTVVFVKHSNGTFRARLGLGPKTDAALPKLTFNTAFEPDVFHLAVANSV
                     GIFIENARDPKMIARLPEWFRRSFDDARAFVLLPVVDETSQTVALLYGDWSYTQEPRR
                     ITQKEMSALNELAKELGRFFGLGQMQEMETM"
BASE COUNT         1183 a         1881 c         2124 g         1285 t
ORIGIN      
        1 cggcgactat cgctacaccg tgcgtcacga cgaagaacgt atcgtgtttc ccaacgcgac
       61 ggtcaagccg ggtttgcgcg cgggtctgct ggtcgtcgag accactgagg aaacgctctc
      121 gaagctcgtc tgaatgctcg cgcctctgta caatcccgcc ctcgcggcta ttgcgctgca
      181 ccacactggc cgcacgagtt tgaatcgcaa ttttggcgcg gcaatcatgc ttgcccggat
      241 tcagctctat cttattcaat accgtagagg aacacccgta atgaagatga attggcgaaa
      301 tatggctgcg ctcgcgctgt tcgcgacggc gacggcatcg ctcacgctca cggctggcac
      361 ggcctcggcg gcggatatca aggaagtgcg cttcggcgtc gaggcgtcgt acgcgccgtt
      421 cgaatcgaag tccccggcgg gcgagctgca aggtttcgat atcgacgtgg gcaatgccgt
      481 gtgcgcaaaa ctgaaggcga agtgcgtgtg ggtcgagaat tcgttcgacg gcctgattcc
      541 ggcgttggca gcgcgcaagt tcaacgcgat caactcggac atgacgatca cggaacagcg
      601 ccgccaggcc atcgacttca ccgatccgat ctacacgatc ccgaatcaga tgatcgcgaa
      661 gaagggcagt ggtctgctgc ctacggcggc ctcgctcaag ggtaagcacg tcggcgtgct
      721 gcaaggcacg attcaggaag cgtatgcgaa ggcgcgctgg gcgccggccg gtgtcgacgt
      781 cgtgccgtat cagacgcagg atcagatcta tgccgacctc gcgtcgggtc gtctcgacgc
      841 cgcgttccag gacgcggaag cagcttcgaa gggtttcctg aagaagccgc aaggcgcggg
      901 cttcgaattc gcgggcccgg ccgtcactga cgaaaagctg ctcggcgcgg gcgtgggctt
      961 cggtatccgc aagggcgata aggcgttgaa ggacgccttg aaccaggcgc tcaaggaact
     1021 gaaggccgac ggcacgatcg accgcttcgc cgcgaagtac ttcgacgtga aggtcgtgtt
     1081 gaagtaagac ccgagtgtcg ttctccgttg tacgcaaacc ggccgcctgc gcggccggtt
     1141 tgcattgcgg cgtcacttta cacagtcgag tggcgaatgc gtggctaaag ggcgggttga
     1201 actgctcgcc agatgcagga ccgtctgcgc gacttcgtgc gcggtcgcga acgaggtgtc
     1261 ggcgccgccg cgaggcgcca cgtgcctgac cacatcgtcg tccccggcac cgtggcaaac
     1321 cagatacgcg tgcacattgt gcggcgcgcc ttctttggca agtacgcgag cgatcccgag
     1381 taacgcgtgt ttcgccgcgg cgtacgcgca catcagcggc gaggtgtcct gagcatccac
     1441 cgagccgatg tagatcacaa cgccgccacg gtcgtcccgg tacatgtgcc tgagcgccgc
     1501 gcgggtagtc agaaacgccc cgtcgacgtg ggtcgcctgc atcttccgcc agtcggaaaa
     1561 cgaattgctt tcaagcggat tgaggatctg gacgccggcg ttcgcgacga ggacatcgag
     1621 cgcgccgaac tgtgcgacca cccgatcgat gccctcgttg acagcgtcct cgttcgtgac
     1681 gtccatggcc atgcagacgg ccgtgccgcc gctctttcgg atctgttgta caacttcctt
     1741 gactccctgc gggttgaggt cggcgacgcc gacaaccgca ccggcgcgtg ccagcgccag
     1801 tgcgatctgt ttaccgacgc cgccgcccgc gccggtcacg acagcgatct tgccgatgac
     1861 actgttcatc atctagatat gtttggtcag actgccaaag aagttcgatg aagaggctcg
     1921 cacacggcga ggagccggcg cagcgacgat ttgcgagtga tccagatagt cgaatacccg
     1981 ttcgcccgtt ccgagtgtga gatcggcaat cagatggcgt gcctcgacga tttcgagcac
     2041 gggcaggtcg gccgccggcg cgagcgcatg gggtgagagt tgcagcgacg aaggcccggt
     2101 ccaggcgcct ttcagttcga tattgtcgag gtagtggcgc accagttcac acacgcgcgg
     2161 cttgccgtcc acatgcggaa gcactttcag cagggagttc ggcatctcca gccgcttgcg
     2221 ctctttggcg aggttcagcg agtagtgctt gtagcccatg gtggcagtgg cgatgcgcac
     2281 cgggccgtag tcgagcgtgc cgatcagcgt gtcggtgtga acggcgagtt caggtcgcgc
     2341 gagcttcttc gggaagcccc acagttcgcg gccggcggcg atgggcggat gatcgtccac
     2401 gtacatggcc agcgtgtagc tgccggcgac gccgtcgagg gtgaccggga tgacctggcc
     2461 gctctcggtg tagtcgccga atcccgtgga atccggcgtg cggatgaatt cgaaatgcac
     2521 cagcgggtcg tgcacctcga gtggctccgg cacgaccgcg cgcagcttgg ccgggtccgt
     2581 gcgatacgtg acgatcagga gttcccggtc gacgaaatgg tacgggccca tggagaaggc
     2641 ggggcttgtc accggcatgg aaaacgcatt gggcagaatg gagttgattt tcatggtgag
     2701 ggtcgtctct ggtggtggcg gggaaggtgg ggcgttattc gaatgagttt gtgctttgtt
     2761 atgtcattcg tataacgaag taattgtgat gaaaggatgg ccttaagttg gaaggcgtat
     2821 ctctgatgcg ggaatggatc gggtcctggg ctgggatgga ttgattctag cgtgcactgt
     2881 ccttttgatt attcacaata ccagcatcaa attttttcga ttattggcac tcgttcttaa
     2941 cttggcatct cgaaataaga aacggccgca agcggccgtt tatcagaatg actcggcgcg
     3001 ctgtggcgcc gaaggcgtcg gtgactaatg ctatttcaga taatcgaata ccacttcacc
     3061 gagtccgagc gtcagatcgg caaccagatg gcgcgcctcg acgatctcga gcaccggcaa
     3121 ttccgccacc ggggccagcg cgtgcggtgc caattgaagc gcggcgggac ctgtccatgc
     3181 acctttaagg gtgatgtcct gcaagtagta gcgtacgagt tcgcaaatgc gcggcgtgcc
     3241 gtccacgtgc ggaatcactt tcagcaggaa gttcggcgtc tccagacgtt tctgttgctg
     3301 ggcgagatcc agttgccgat gtttgtagcc catggtgccg gtggcgatgc gcaccgggcc
     3361 gtagtccagc gtgccgacga gcgtgtccgt gtgcacttcg agaacgggct tggcgagctt
     3421 cttcggaaag ccccacaatt cgcggccacc ggcaatcggc ggatggtcgt ccagatacat
     3481 cgcgagcgta tagccgcccg ccacgccgtt gtacgacacc gggatcacct ggccgctttc
     3541 cgtgtagtcg ccgaagccgg tcgaatccgg catgcgaatg aattcgtagt gcaccagcgg
     3601 ctcaccgatc tggagcggct cgggcaccac ggcgcgcagt ttgtccggat ccgtgcggta
     3661 ggtgatgatc aggaattccc ggtcgataaa acgatagggg cccattggaa acgccgggct
     3721 cgtgatcggc atcgcgaagg catttgacag cacgcttttg acgtccatga ttttccttgg
     3781 tggttaaagg cgggtagcgc gggcgatgga acgcttttgc actgcacagg agtatcgtcg
     3841 attcttttcc agcctttatt gagacgggca cccaattatt tcgagatatt gcggcgctga
     3901 catgtccgcg cacggtggcc tgcgctagaa tcgccgggcg caccgcacgc gccacgggcc
     3961 tcaggccttg gatgcgcgcg attggtgcag caaaaaccaa aatacggcag gaagcgcctg
     4021 ggcggcgctt cgcggggagc ggaatgacca ccatcgcaat cgagaatccg ggccagacag
     4081 acgccacggc cgatgcacaa ttgaatgcca gcttcgcgcg tcagccgatt ctgaatcggg
     4141 acggcatgct gtgcggctac gagatcaaag tgcgcgcgcc ggcgttgccg gcgggcgtcg
     4201 aagcgggcgc gggcgcggct gcggcgcttg ccggtgattc ggctacggat gccgacgcct
     4261 tgccgggccg cgcgcctgag gcgacgcaac tattggtgcg agccattgtt cgtggactga
     4321 tccatagcaa tgtacgcggt gcgctcaccg ggcatccggc gtacgtcgac gttagccgcg
     4381 agatgttgtt cgacgattcg atcctgcgtc tgccggctgc gcgtttcatg ttcgaactgt
     4441 cgccgtcgat ggaggttgac gacgcgctgg tcgcgcggat cgttcagttg cacgcgcggc
     4501 gctatcgttt tgcgctcgac gaagtgaccc agcccaacga gacgttcgcc aaactgctgc
     4561 cgtacgcgga ggtcgtcaag atcgatttca tgcatgcccc gcgggcgttg ttgcccaagc
     4621 tgacgagcgt gctcaaggcc gcgggcaagc tgttggtcgc gctgggcatc gatgcccagg
     4681 acgacttcga aatggcgcat gaactcggct tcgatcggtt tcagggttac tttttcgcgc
     4741 gtccgcagac gtcgatgatg cggcgtgtca gcgcgccgcg ccacgccttg ctgaatctgc
     4801 tgcaactgct ggcgggcgag ccgaccgtcg cgcaactcga agctgaactg aagctcaatc
     4861 ccgtgctggt gatgcatctg atgaagctcg cgaattcgag cggcctcgct gtcggctaca
     4921 aggtcagtac gctgcgcgaa gcgatcaatg cgaccggcac caatcggatt gcccgttgga
     4981 cccagttgct gctctacgcg gacggccgca aggtcgcact ggaagacgat cccttgctgc
     5041 aactcgcggc gacccgggcg cgcttcatgg aactggccat tgaacgtttg cccgaggcgg
     5101 ggcgggatga agcggatgcg gcgtttctga cgggcgtgtt ttcgtttgtc gacgcggtgt
     5161 ttggtggttc acttgaaagc acgctgaatg tgctgacgct gtcgcggccg attcaggcgg
     5221 gcattttgca tcggcagggc gtgttggggt tgctgctgac ggtggttgag gcgttggaac
     5281 gcggggcgtg ggatgaggtt gacagccttt gtgcacggtt gcagccgttg acggcggagg
     5341 atgtcgcggc gcttgggtta gcggcggggg cgtgggctgg ggtcgcggat cggagtgcgg
     5401 aggggttgga gatgattgag gattggtcct cggaggattg aagcggaagc tgctggcatg
     5461 cgctgcatgc cagcctcggc aaactcaatt tagggcaaat acaaagcggg tgaatgtgaa
     5521 gcaccgtgat caacgatcac gccacgtctt cacatcgtct ccatttcctg catttgcccc
     5581 agaccaaaaa aacgtcctaa ctccttggcc aattcattca acgcgctcat ttccttctgc
     5641 gttatccggc gtggctcctg cgtataagac cagtcgccat ataacagcgc caccgtctga
     5701 ctggtttcgt cgacgaccgg cagcagcacg aaagcgcggg cgtcgtcgaa cgaacggcgg
     5761 aaccattccg gtaatcgcgc gatcattttc gggtcgcgtg cattctcgat gaaaatgccg
     5821 accgagttcg cgaccgcgag atgaaacacg tcgggctcga aggcggtgtt gaaggtcagc
     5881 ttcggcagcg ccgcgtcggt cttcggaccg agcccgagac gcgccctgaa ggtgccgttg
     5941 ctgtgcttga cgaataccac cgtgcgcgcg aagccgagcg ccgccaacac ggtttccgcg
     6001 gccagcgcca gcgccggtgc gagcgcgctg ccgtcgggca ggccgcgcag atcgcccact
     6061 cccgccgcga tgcgcgcttc cggattgagc gcctcgcgcg cgattgcgtc cgcattggcg
     6121 cgcaactcga cgatttcggc catcacgccg tcgccgcctt cttcacgggc gagtgcgatg
     6181 ctcatctgat gcagtacttc cggatcggta ttcagcgcgt ggctgtatgc ctgcgccagt
     6241 ccggcgatgc gttcttcgcg ctgccagtcc ggcatgttct gctgggtcag cacgtcggca
     6301 accgccgtcg aatagttggt gatggcgcgc agccactgca cctggcgcgg ctgctcgaca
     6361 tccagcggat cgaactcgcc catgccggca cggatcatgt cgggcaagcg ccagcgcacc
     6421 gcggcttcgg cgccgatctc gtcgaacgtg acgccgagca ccagcacgca ggc
//
LOCUS       DATASH010001112         2823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5336454,
            whole genome shotgun sequence.
ACCESSION   DATASH010001112 DATASH010000000
VERSION     DATASH010001112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5336454"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="VHV99_29555"
     CDS             complement(<1..704)
                     /locus_tag="VHV99_29555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3639555.1"
                     /translation="MSTITTKDGTEIFYKDWGKGRPVVFSHGWPLSADAWDAQMLFLG
                     NQGFRVIAHDRRGHGRSAQTWDGNDMDTYADDLAALIDHLDLQDATLVGHSTGGGEVA
                     HYIGRHGTKRVAKAVLIGAVPPLMLKTEGNPEGTPIDVFDGIRKGVEADRSQFFKDLA
                     LPFYGYNRPNAKLSQGVIDSFWAQGMVGSIKGLYDCIKQFSEVDYTEDLKKIDVPTLV
                     LHGDDDQIVPIDAAGR"
     gene            1420..1752
                     /locus_tag="VHV99_29560"
     CDS             1420..1752
                     /locus_tag="VHV99_29560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012365190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX3639556.1"
                     /translation="MAFTIGFYNEKVKVNVASLPKTLLARFLALADRTEEYGPNLGEP
                     HTASMGGGLFEMRLKGAEGIARVFYCAIVERKIVMLHSFVKKSQQTPRNELDVARKRL
                     LEIRNGNL"
     gene            1739..2026
                     /locus_tag="VHV99_29565"
     CDS             1739..2026
                     /locus_tag="VHV99_29565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006749372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX3639557.1"
                     /translation="MATYKAVRERALADPEVRAEYERLNREEFALLDQMLAARRDAGL
                     TQAQVAERMGTQAPAITRLERALASGQHSPSVDTLRRYAAACGKQLFISIA"
     gene            2314..>2823
                     /locus_tag="VHV99_29570"
     CDS             2314..>2823
                     /locus_tag="VHV99_29570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006481318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEX3639558.1"
                     /translation="MENSRKTTRFFDAPRTAAKNSPFPYSGATCQAQCAPTTVDARSP
                     PPGPTHLKHLTLMNSALSAFDLARMQFAFTVSFHIVFPALSIGLASFIAVLEWRWLKT
                     GRAYYKDLCLFWSKIFAVAFGMGVVSGVVMSYQFGTNWSGFSSFAGPITGPLLMYEVM
                     TAFFLEAGFL"
BASE COUNT          566 a          876 c          795 g          586 t
ORIGIN      
        1 ttgcggcctg ccgcgtcgat cggcacgatc tggtcgtcgt cgccatgcag gaccagagtc
       61 ggcacgtcga tcttcttcag atcttctgtg tagtcgactt cggagaactg cttgatgcag
      121 tcgtacaagc ctttgatcga accgaccatg ccttgcgccc agaacgaatc gatcacgcct
      181 tgcgacagct tcgcgttcgg acggttgtag ccgtaaaagg gcagcgccag atccttgaaa
      241 aactgcgaac gatccgcctc gacacccttg cgaatgccgt cgaacacgtc gatcggcgtg
      301 ccttcaggat tgccttctgt cttgagcatc agcggcggta ccgcgccgat cagcacagcc
      361 ttcgccacgc gtttcgtacc gtggcggccg atgtaatgcg cgacttcgcc gccaccggtc
      421 gaatggccga ccagggtggc gtcctgcagg tcgaggtggt caatcagcgc cgcgagatcg
      481 tcggcgtagg tatccatgtc gttgccgtcc catgtctgcg ccgaacggcc atgaccgcgc
      541 cggtcgtgcg cgatcacgcg aaacccttgg ttgccgagga acagcatctg cgcatcccac
      601 gcgtccgcgg acaatggcca gccgtgtgag aagacaaccg ggcggccctt gccccagtct
      661 ttgtagaaga tttccgtacc gtctttcgtg gtgatcgtgc tcatctgctg actcctttgg
      721 atgaagtctg ccgttgccgg cagggggatg aagcgtgcct ctctaaccat tcgggcggca
      781 caagccgtgc cgcccgacga aaaccgctca atcgcgaatg caggacgaac tcgacgcgca
      841 ttcaggcaga acatgggaga aaaagtaatc ggtcgatgcg cgatgaggcg ctcattcaag
      901 cgcggcacgg tgcgcaacag gctttgatga cggactcacg aagaaccgaa tcggttcgcg
      961 cgccaatcga aatgactatc aggaatgaac gctcggatgg cgatgcagcg ctcgcccgtt
     1021 tcgggcctgc ggcaagccga tataaaccgc tattagctgc ttgcatggta gaagcaaact
     1081 agccggatgg ctagctaacc ggctaataga aagtcggcgc gaacggcgtt attgtcggtt
     1141 gttgacgagt ggacccggtc cgccgcgtcg cttcaatcaa tccgcgcaaa aggagacctg
     1201 cgccgtgccg cgcccgatat cgcgtcccgc acgacccacc cagccgaaca cctggttgcg
     1261 cctatgccgt cgtaaccgta catcgcggtc ctgctttcgc cctcttcacg tcgttcgatt
     1321 ccaagcctcg caacgcctcc ggtttttgct aatcagcagc aagcaccatc aaggacgcct
     1381 gcttgcgcgt taataacata tttgttatca tcaattccaa tggcatttac gatcggtttc
     1441 tacaacgaaa aggtcaaagt gaacgttgcc tccctgccga aaacactgct cgcgcgcttt
     1501 ctcgcgttgg ccgatcgcac ggaggaatac ggtccgaatc tcggcgagcc gcacacggcg
     1561 tcaatgggcg gcggtttgtt cgagatgcgc ctcaaaggtg ccgagggcat tgccagagtg
     1621 ttctactgcg cgatagtgga acggaaaatc gtcatgcttc actctttcgt gaagaagtcg
     1681 caacaaacgc cgcgcaacga actggacgtg gcgcgcaaaa gactgctgga gatcagaaat
     1741 ggcaacttat aaggcagtgc gcgaacgggc gctcgccgat cccgaagtac gtgccgaata
     1801 cgaacggttg aaccgcgaag aattcgcact ccttgaccag atgctcgccg ctcgtcgcga
     1861 cgccggcttg acgcaagcgc aggtcgcaga gcgcatgggc acccaagcgc cggccatcac
     1921 ccggctcgaa cgggcgcttg cttcggggca gcactcgcct tctgtcgata cgttgcgccg
     1981 atacgcggct gcgtgtggca agcaactgtt tatctcgatc gcatagctgg cgtggccggg
     2041 acttaacccg ccgcccgtcg cttattcctg ctgttcctgc gctcgatcaa ggcgggtgcg
     2101 tgcatcgcat ccgtcaatgg aaaggccgct cggctgacct tggcagttga gaacccggtg
     2161 ctcctgtcaa gcgccgtcct cacgcggcgt tgcgcattcg gttagctacg ctgcagcgcg
     2221 gtcgattgcc gcagttctgc ccgatccgcg taagttggac gcaaagacat actcaggaaa
     2281 acttgagacc tatcaaggcc ttgccgtctc aaaatcgaaa acagccgcaa aacaacgcgg
     2341 ttttttgacg cacctcgcac cgcagcaaaa aattctccct tcccctactc tggcgccacc
     2401 tgccaagcgc agtgcgcacc gacaacggtc gacgcgcgta gtccgcctcc aggcccgacg
     2461 cacctaaagc acctcacgct catgaactcc gctctatcgg cattcgatct cgcccgcatg
     2521 cagtttgcgt tcaccgtctc gtttcacatt gtttttccgg cgctcagcat cggcctcgcc
     2581 agcttcatcg ccgtcctcga atggcgttgg ctgaaaaccg gcagggcgta ctacaaagat
     2641 ctttgcctgt tctggtcgaa gatcttcgca gtcgccttcg gtatgggcgt cgtttcgggt
     2701 gtcgtgatga gttatcagtt cggcaccaac tggtcgggct tctccagttt cgcgggcccc
     2761 atcaccggcc ccttgctgat gtacgaggtg atgaccgcgt tcttcctcga agcgggcttc
     2821 ctc
//
LOCUS       DATASH010001113         2892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1974361,
            whole genome shotgun sequence.
ACCESSION   DATASH010001113 DATASH010000000
VERSION     DATASH010001113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1974361"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="VHV99_29575"
     CDS             <1..664
                     /locus_tag="VHV99_29575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004524547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HEX3639559.1"
                     /translation="TSLFVFDPNLVFFVVDNLFGGDGRFHTRVEGRDFTATEQRIIMK
                     LLNLTFEHYTASWKSVRPLQFEYVRSEMHTQFANVATPNEIVIVTQFSIEFGTTGGTL
                     HICMPYSMIEPIRDILSSPIQGEALEVDRRWVRVLSQQVQAAEVELTADLAQVPVTFE
                     QILNMRKGDVLPINIPEHITAKVDGVPVMECGYGIFNGQYALRVQKMISAAETMKDGG
                     YE"
     gene            657..1121
                     /gene="fliN"
                     /locus_tag="VHV99_29580"
     CDS             657..1121
                     /gene="fliN"
                     /locus_tag="VHV99_29580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HEX3639560.1"
                     /translation="MSDLNAKTEAELAAGEAQLAADAASAEDDAAMADWASALAEQND
                     NTEVSATTAGVFQPLSKVEPTSTRNDIDMILDIPVQMTVELGRTKIAIRNLLQLAQGS
                     VVELDGLAGEPMDVLVNGCLIAQGEVVVVNDKFGIRLTDIITPSERIRKLNR"
     gene            1118..1819
                     /gene="fliO"
                     /locus_tag="VHV99_29585"
     CDS             1118..1819
                     /gene="fliO"
                     /locus_tag="VHV99_29585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020415483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HEX3639561.1"
                     /translation="MKRVAGRAMLRFCHASRVVFNRAVLAATGTTAAATSALKLARTS
                     VATSALIAVATPALTSVAIFAPSAAHAADMNAVNNAARIASGVGAGSAVPALGVGAVL
                     QTIVGLLVVIGLVFACAWLARRFGLQPAGRAGLVKTIGGASLGGKERVAVVEIGDTWL
                     VLGTAPGNVRLLHTMPAGSAALDPVAGTQAGAPGASASPGAGAALPGTFGQRFRDALK
                     GEVGKRFNGQGSGDR"
     gene            1819..2652
                     /gene="fliP"
                     /locus_tag="VHV99_29590"
     CDS             1819..2652
                     /gene="fliP"
                     /locus_tag="VHV99_29590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434695.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HEX3639562.1"
                     /translation="MQFSLQSAQSVRQAHATRMSRDASTVLPVLRRLAPVVLAALMLA
                     LPTLSFAQAAGLPAFNTSPGPNGGTTYSLSVQTMLLLTMLSFLPAMLLMMTSFTRIII
                     VLSLLRQALGTTTTPPNQVLVGLALFLTLFVMSPVLDKAYNDGYKPFSDGTIPMETAV
                     NRGLAPFKTFMLRQTRESDLALFARISHAAPMQGPEDVPLSLLVPSFVTSELKTGFQI
                     GFTIFIPFLIIDMVVASVLMSMGMMMVSPATISLPFKLMLFVLVDGWQLLLGSLAQSF
                     V"
     gene            2763..>2892
                     /locus_tag="VHV99_29595"
     CDS             2763..>2892
                     /locus_tag="VHV99_29595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004199882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HEX3639563.1"
                     /translation="MNQESVMTLAHQAMYVGLLLSAPLLLVALVVGLVVSLFQAATQ"
BASE COUNT          494 a          926 c          917 g          555 t
ORIGIN      
        1 cacgtcgctg ttcgtgttcg acccgaatct ggtgttcttc gtggtcgaca atctgttcgg
       61 cggcgacggg cgcttccaca cccgcgtcga aggccgcgat ttcacggcaa ccgagcagcg
      121 catcatcatg aagctgctca atctcacgtt cgagcactac acggcgtcgt ggaaaagcgt
      181 gcgtccgctg cagttcgaat acgtgcgctc ggaaatgcac acgcagttcg ccaacgtggc
      241 gacgccaaac gaaatcgtca tcgtcacgca gttctcgatc gagttcggca cgacgggcgg
      301 cacgctgcac atctgcatgc cgtattcgat gatcgaaccg atccgcgaca ttctctcgtc
      361 gccgattcag ggcgaggcgc tcgaagtcga ccgccgctgg gtccgcgtgc tgtcgcagca
      421 ggtgcaggca gcggaagtgg aactgaccgc cgacctggcc caggtaccgg tcacgttcga
      481 acagatcctg aacatgcgca agggcgatgt tctgccgatc aacattcccg aacacatcac
      541 ggcgaaggtc gatggcgtgc cggtgatgga atgcggctac ggaattttca atggtcagta
      601 cgcgttgcgg gtccagaaga tgatcagcgc agccgaaacg atgaaggatg gtggatatga
      661 gtgacctgaa cgcaaagacc gaggcagagc tggccgccgg cgaggcgcaa ctggccgccg
      721 acgcagccag cgccgaagac gacgcggcga tggccgactg ggcgagcgcg ctggccgagc
      781 agaacgacaa cacggaagtc agcgcgacca cggccggcgt gttccagccg ctttcaaagg
      841 tcgaaccgac ctcgacgcgc aacgacatcg acatgatcct ggacattccc gtccagatga
      901 ccgtcgaact cggccgcacc aagatcgcga tccgcaacct gctgcaactc gcgcaaggtt
      961 cggtcgtgga actggacggc ctggcgggcg aaccgatgga cgtgctggtg aacggctgcc
     1021 tgatcgccca gggcgaagtg gtggtggtga acgacaagtt cggcatccgt ctgacggaca
     1081 tcatcacgcc gtcagaacgc atccggaagt tgaatcgatg aaacgcgttg ccggtcgcgc
     1141 catgctgcgg ttttgccatg catcgcgcgt tgttttcaat cgcgctgtgc tcgcagcaac
     1201 gggaacgacg gcagcagcca cctctgcgct gaagctcgcg cggacttcag tggcgacctc
     1261 ggcgctgatc gccgtggcca ccccggccct gacctcggtg gccatcttcg cgccatcggc
     1321 cgcgcacgca gccgacatga acgcagtcaa caacgccgcg cgaatcgcct cgggtgtggg
     1381 tgccggctcg gccgttccgg cgctcggcgt cggcgcggtg ctgcagacca tcgtcggctt
     1441 gctggtcgtg atcggtctgg tgttcgcctg tgcctggctc gcgcgccgct tcggcttgca
     1501 acccgccggt cgcgccggcc tcgtgaagac gatcggcggc gcctcgctcg gcggcaagga
     1561 gcgggtcgcg gtggtcgaga tcggcgatac gtggctcgtg ctcggcacgg cgcccggcaa
     1621 tgtgcgcctg ctgcacacca tgcccgccgg ttcagccgcg ctcgatccgg tcgcaggcac
     1681 gcaagcgggt gcacccggcg catcggcttc accgggcgcc ggcgcggcgt tgccaggcac
     1741 cttcggccaa cgctttcgcg atgccttgaa aggcgaagtg ggcaaacgtt tcaatgggca
     1801 aggcagcggg gatcggtaat gcagttcagt cttcagtcag cgcaatcagt acgtcaagcg
     1861 cacgccactc gcatgtctcg cgacgcctcg acagtgcttc ccgttttgcg ccgtctcgcg
     1921 cctgtcgtgt tggcggcgct gatgctcgcg ctgccgacgc tgtcgttcgc gcaagccgcc
     1981 ggcttgccgg ccttcaatac gagcccgggt ccgaacggcg gcacgaccta ctcgctgagc
     2041 gtgcagacga tgctgctgct cacgatgctg tcgttcctgc cggcgatgct gttgatgatg
     2101 acgagcttca cgcgcatcat catcgtgttg tcgctgctgc gccaggcgct cggcacgacg
     2161 accacgccgc ctaaccaggt gctggtcggt ctcgcgctgt tcctcacgct gttcgtgatg
     2221 tcgccggtgc tcgacaaggc ttataacgac ggctataaac cgttctccga cggcaccatc
     2281 ccgatggaaa cggcggtgaa ccgcggcctc gcgccgttca agacgttcat gctgcgccag
     2341 acccgcgaaa gcgatctcgc gttgttcgcg cgcatctcgc acgcggcgcc aatgcaaggt
     2401 ccggaagacg tgccgctctc gctgctggtg ccgtcgttcg tgacgagtga gctgaagacg
     2461 ggcttccaga tcggcttcac gattttcatc ccgttcctga tcatcgacat ggtggtggcg
     2521 agcgtactga tgtcgatggg gatgatgatg gtgtcgcccg ccaccatctc gctgccgttc
     2581 aagttgatgc tgtttgtgct ggtcgacggt tggcaattgc tgctcggctc gctcgcgcag
     2641 agctttgttt aacggcgccc ctggttgggt cacttgtcac tgggttgcgt cacctggttg
     2701 cgtcacctgg ttacgtccat tagccacgcc actccaccct tcacgcttcc agtcgactcg
     2761 ccatgaatca agaatccgtc atgacactcg cgcaccaggc catgtatgtc ggcctgctgc
     2821 tctccgcgcc cttgctgctg gtcgcgctcg tggtcggtct ggtggtgagc ctgttccagg
     2881 ccgcgacgca ga
//
LOCUS       DATASH010001114         2331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_1184836,
            whole genome shotgun sequence.
ACCESSION   DATASH010001114 DATASH010000000
VERSION     DATASH010001114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1184836"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="VHV99_29600"
     CDS             complement(<1..573)
                     /locus_tag="VHV99_29600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX3639564.1"
                     /translation="MKTIKGPAIFLAQFMGDEVPFDNLAHLAGWAASLGFKGIQVPCD
                     TRLIDLEQAAASQAYCDELREIADDAGVAITELSTHLQGQLVAVHPAYDTLFDGFAAQ
                     HVRGDPTARTQWAVDQMKLAAQASQRLGLNTHVSFSGALAWPYLHPWPQRPAGLVEAA
                     FDELARRWTPILDAFDQAGVDVCYELHPGED"
     gene            complement(672..1634)
                     /locus_tag="VHV99_29605"
     CDS             complement(672..1634)
                     /locus_tag="VHV99_29605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015001951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX3639565.1"
                     /translation="MKQVIRAVGAGMLALGILGTAGVARADEKVTLGVAIPTADHGFT
                     GGIVWWANKAKTDLEKAHPDLKVIVKTAAGAPEQANQLQDLVTVNKINALVIFPFESA
                     SLTQPVAQVKKKGVYVTVVDRGLTDTSAQDAYVAGDNTAFGKIPAEYLAKALGGKGNI
                     VALRGIPTTLDNERWTAFTNVIKNYPDMKILDGKYANWNRDDAFKVMQDYLTRFKHID
                     AVWAADDDMAVGVIKAIEQAKRTDIKIVFGGAGSKGMVKNVMDGAPMIKADVSYSPKF
                     IYDAIKLTAEARLKGEKLPPTTIIPSVLITKENAQQFYFPDSPF"
     gene            complement(1718..>2331)
                     /locus_tag="VHV99_29610"
     CDS             complement(1718..>2331)
                     /locus_tag="VHV99_29610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018431732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3639566.1"
                     /translation="ITKGRIEPFIVTLGTLGIFRAVLTWLADGGALTLDNSLSDLYGP
                     VYYASFFGVPVPIWVFLVVAAGGALILNRTAFGRHVQAIGSNEQVARYAAIRVDTVKI
                     VTYVLLGICVGVATVLYVPRLGSATPTTGLLWELEAIASVVVGGTALKGGEGRVVGTV
                     IGAILLSVIANILNLTSIISVYLNAAVQGVVIIFVAFVQRGRR"
BASE COUNT          438 a          778 c          669 g          446 t
ORIGIN      
        1 gtcctcgccc ggatgcagtt cgtagcacac gtcgacaccg gcctggtcga acgcatcgag
       61 aatcggcgtc cagcggcgcg cgagttcgtc gaaggcggct tctaccaaac ccgccggacg
      121 ctgcggccac ggatggagat aaggccacgc cagcgcgccg gaaaacgaca cgtgtgtatt
      181 cagcccgaga cgttgcgacg cctgcgccgc cagtttcatc tgatcgaccg cccactgcgt
      241 gcgcgcagtg ggatcgccgc gcacatgctg tgctgcaaag ccgtcgaaca acgtgtcata
      301 agccggatgc acggccacca gttgcccttg cagatgcgtc gacagttcgg tgatcgccac
      361 acccgcatcg tccgcgattt cacgcaactc gtcgcaatag gcctgactcg ccgccgcctg
      421 ttcgagatcg atcaggcgcg tatcgcacgg cacctggatt cccttgaagc cgagactcgc
      481 ggcccagccc gccaggtgcg cgaggttgtc gaacggcacc tcgtcgccca tgaactgggc
      541 cagaaagatc gccggccctt tgatggtttt catcgtagat tcctcgaagt agggcgcgcc
      601 tgtcatgcct ctgcggcaga gccacggcaa gcgcgcgcgg catacccaga atcagccaga
      661 atcatcctgg atcagaacgg cgagtcggga aaatagaact gctgcgcgtt ttccttcgtg
      721 atcagcaccg acggaatgat cgtggtgggc ggcagcttct cgcccttcag gcgcgcttcg
      781 gcggtgagct tgatggcgtc gtagatgaat ttcggcgaat acgacacgtc ggccttgatc
      841 atcggcgcac cgtccatcac gttcttcacc atgcccttcg aacccgcgcc gccgaacacg
      901 atcttgatgt cggtgcgctt ggcctgctcg atcgccttga tgacacccac ggccatgtcg
      961 tcgtcggcgg cccagactgc gtcgatatgc ttgaagcgcg tgaggtaatc ctgcatcacc
     1021 ttgaacgcgt cgtcgcgatt ccagttcgcg tacttgccgt cgagaatctt catgtcggga
     1081 tagttcttga tcacgttcgt gaacgccgtc cagcgttcgt tgtcgagggt ggtcggaatg
     1141 ccgcgcaacg cgacgatgtt gccttttccg cccagcgcct tcgccaggta ctcggcggga
     1201 atcttgccga acgcggtgtt gtcgccggca acataggcgt cctgtgcgct ggtgtcggtc
     1261 aggccgcgat ccaccaccgt cacatacacg cccttcttct tcacctgcgc gaccggctgc
     1321 gtgagcgagg ccgattcgaa cgggaaaatc accagcgcgt tgatcttgtt caccgtcacc
     1381 agatcctgca actggttcgc ctgttcgggc gcgccggctg cggttttcac gatcactttc
     1441 agatcgggat gggctttctc cagatcggtt ttcgccttgt tcgcccacca gacgatgccg
     1501 cccgtgaagc catgatcggc tgtcggaatc gcgacgccca gcgtgacttt ttcatcggca
     1561 cgcgcgacgc ctgccgtgcc cagtatcccc aacgccagca tgccggcgcc aactgctcga
     1621 atgacctgct tcatggttgt gtctccgatt gtgaggcgtt catgcgcccc gattctggaa
     1681 cttgccgttg gtttccttga agctcaacgc ctgctgccta ccgccgtccg cgctgcacga
     1741 acgcgacgaa gataatcaca acgccctgca ccgccgcgtt cagatacacg ctgatgatgc
     1801 tggtcagatt cagaatattg gcgatcaccg aaagcaggat cgcgccgatc accgtgccga
     1861 ccacgcgccc ttcgccgcct ttcagcgcgg tgccgcccac caccaccgac gcaatcgctt
     1921 caagctccca aagcaagcct gtggtcggcg tcgccgagcc gagccgcggc acgtacagca
     1981 cggtggcgac gccgacgcag atacccagca gcacgtacgt gacgatcttc accgtatcga
     2041 cacgaatggc cgcgtaccgc gccacctgtt cgttggagcc gatcgcttgc acatggcgac
     2101 cgaacgccgt gcgattgaga atcaaagcac cgcccgccgc gacgaccagg aacacccaga
     2161 tcgggaccgg cacgccgaag aagctcgcgt agtaaaccgg cccgtacaag tccgacagcg
     2221 agttatccag cgtcagcgcg ccgccatccg cgagccacgt cagcaccgcg cgaaaaatcc
     2281 ccaacgtgcc cagcgtgacg atgaacggct cgatgcgtcc tttggtgatc a
//
LOCUS       DATASH010001115         4486 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_985667,
            whole genome shotgun sequence.
ACCESSION   DATASH010001115 DATASH010000000
VERSION     DATASH010001115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4486
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_985667"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..600
                     /locus_tag="VHV99_29615"
     CDS             103..600
                     /locus_tag="VHV99_29615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639567.1"
                     /translation="MSSKITKQTQPDSRPPVDALQYEKLALQAFNLCTRQLNQLETLV
                     TFAATICRNPAITLEERRRQQTLLELLVHTGEQYQQELTCDRELFQVIALDAKGVAQS
                     RITAKRAACLLEESAQIAAEAQAEALAHAQAATIKASQHNQPPAKDTPSARAEVAQQP
                     NGAPH"
     gene            complement(713..2425)
                     /locus_tag="VHV99_29620"
     CDS             complement(713..2425)
                     /locus_tag="VHV99_29620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX3639568.1"
                     /translation="MKIGARLALAFTVTFLLTFALSAVSLNELRGFNQGVNNATRARM
                     PVLLDLHLLLETTRGQAVMERDALAALASNDAQAVSTNLQSIGNGHEAVSDAAKEIER
                     LVDTDQGKALWARIGSVREAYLSNRAAIIAQIKAGDAEGARQKLLSEQVPLENQYLSA
                     VQALIDYVGNQVVMERDASSASYDAARLLVLVLTALALAVCAVCAVLVTRSIVRPLRE
                     AVDSAEKVAHGNLVTEIDVDGKDETRQVLRSLRTMTEGLSNTVGEIRMAAESVMTGAQ
                     QIAQGNTELSSRTEEQAASLQQTAASMEELTATVRQNSENARQASGFADDARAVANEG
                     SAIVGKVVETMAGIEESAGKIADITGMIEGIAFQTNILALNAAVEAARAGEQGRGFAV
                     VASEVRSLAQRSSVAAKEIKALIEISGSRVQTGTELVARAGQTMGNVGTAIHRVTDIM
                     GEIASASAEQSRGIEQVNQAISQMDEVTQQNAALVEEAAAAAGSLLDQSRRMRAAVAV
                     FRTAQTDVVAPGVRPRAVVKATAASRAGPVVERAGPATKVAAPVPRRVEPAVKVEGAD
                     WETF"
     gene            2919..>4486
                     /locus_tag="VHV99_29625"
     CDS             2919..>4486
                     /locus_tag="VHV99_29625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(methylthio)propionyl-CoA ligase"
                     /protein_id="HEX3639569.1"
                     /translation="MTTPLLGQMMDVPLTVSSLLAHASRHFGTTEIVSKRIEGDVHRY
                     TYRDCEKRAKRLAQALIALDVQPGERIGTLAWNGYRHLEAYYGTTGFGAVCHTINPRL
                     FPEQIAWIINDANDSYVLFDTTFAQLIDCLAPLCPEVRGWIAFADDAHLPAVQRPLPL
                     ISYETLLNPHDGVFDWPLLDERVASYLCYTSGTTGNPKGALYSHRSTVLHAYAGSLPD
                     AMCLSARDSVLPVVPMFHANAWGIPHAAPLTGAKLVLPGKDLDGKSLFELMQAERVTY
                     SSGVPTVWLGLLNYMKEAGVRLSSLQRVAIGGSACPPAMLRAFEEYGVEAVHGWGMTE
                     MSPLGTLATLNWEQSQRPPEEQRRLREKQGHAIYGVDMKIVGADGRDLPWDGVAFGEL
                     HTRGPWVINRYFQKDDSPLVDGWFPTGDVATIDRDGFMRITDRSKDVIKSGGEWISSI
                     EIENIAIAHPAVTEAACIACAHPKWLQRPLLIVVKRKDAEVTRDELLAFYEGKVAKWW
                     MPDDVVFVNELPHTA"
BASE COUNT          867 a         1467 c         1307 g          845 t
ORIGIN      
        1 cgccggtttg gtgaaatccc ggtctgctaa cctgctacgt gcccgctcac ccctttgcaa
       61 gcgagtgtcg ggcgatccga gatgtacgag ggaggtcgca ccatgagcag caaaatcaca
      121 aaacaaaccc aacccgattc acgcccgcca gttgacgcgc ttcaatacga gaaactcgca
      181 ttgcaagcat tcaacctttg cacccgccag ttaaaccaac tggaaacgct tgtcactttt
      241 gcggccacaa tatgtaggaa tcctgccatc acgctagagg aacgtcgtcg ccagcagacc
      301 cttcttgaat tgctcgtcca cacaggcgag cagtatcaac aggagctgac atgcgaccgc
      361 gaactgttcc aggtgattgc gctggatgca aagggcgtcg cgcaaagccg catcaccgca
      421 aagcgtgcgg cgtgccttct tgaagagtcc gcgcagatcg cagcagaagc ccaagccgaa
      481 gccctggcgc atgcgcaagc tgcgacaata aaagcaagcc agcacaacca gccgcccgcc
      541 aaagacacac cgagcgcaag agcagaagtc gcccagcaac cgaacggcgc accgcattaa
      601 cccatcagaa gcggccagtg cgtcacatag acgcgctgaa taaaccgtcg acgcgccgcg
      661 actgaccgtg gcggcacatc aacacaatca attcgacagc attgcgcccc tgtcagaacg
      721 tttcccaatc cgcgccctcg accttcaccg ccggttccac gcgtcgcggc accggcgcgg
      781 cgactttcgt ggccggtccc gcacgctcca ccacaggacc agcccgactc gccgctgtcg
      841 ccttcacaac agcccgcggg cgcacccctg gcgcgacgac atcagtctgc gccgtccgga
      901 acaccgccac cgcggcgcgc atccgcctgg actgatccaa cagtgagccg gccgcggcgg
      961 ccgcctcttc gaccagcgcc gcgttctgct gcgtgacttc atccatctgg ctgatcgcct
     1021 ggttcacctg ctcgatgccg cggctctgct cggccgaggc tgacgcgatc tcgcccatga
     1081 tgtcggtcac gcgatgaatc gctgtcccta cgttgcccat cgtctgtccg gctcgcgcga
     1141 ccaactccgt tcctgtttgc acgcggctgc ccgagatctc gatcagcgcc ttgatctcct
     1201 tcgccgcgac tgacgagcgc tgcgccagag agcgcacttc gctggctact acggcaaagc
     1261 cccgaccctg ctcgcccgcc cgtgccgctt ccaccgccgc gttgagcgcg aggatgttgg
     1321 tctggaatgc gataccctcg atcatgccgg tgatatcggc gatcttgccc gcgctttcct
     1381 cgatgcccgc catggtctcg accaccttcc cgacgattgc agaaccttcg ttggcgacag
     1441 cgcgcgcatc gtccgcgaag ccgctcgcct ggcgagcgtt ctccgagttc tgcctgaccg
     1501 tggccgtcag ttcctccatg ctcgccgccg tttgctgcag cgacgcggcc tgctcctccg
     1561 ttcgcgaaga cagctcggtg ttgccctgtg cgatctgttg tgcgcccgtc atgaccgact
     1621 cggcggccat gcggatctcg ccgacagtgt tagataggcc ttcggtcatg gtacgaagtg
     1681 atcgaagcac ctgtctcgtc tcgtccttgc catccacgtc gatctccgtg accaggttgc
     1741 cgtgcgcgac tttttcggcc gaatcgacgg cttcacgcag cggccggacg atcgagcgcg
     1801 tgacaagtac cgcgcagacc gcacagacag ccagcgcgag cgccgtgagc accagtacga
     1861 gcaggcgggc ggcatcatac gatgcgctcg atgcgtcgcg ctccatcacg acctggttgc
     1921 cgacgtagtc gatcaaggct tgtactgcgc tcagatactg attctccagc ggcacctgtt
     1981 cgctcagcaa cttttgccgt gccccttcgg cgtcgccggc cttgatctgc gcgatgattg
     2041 ccgcccggtt ggacagatac gcctcacgca cggatcctat ccgcgcccag agcgccttgc
     2101 cctggtccgt gtcgaccagt cgctcgattt ctttggctgc atcggagact gcctcgtgac
     2161 cattgccgat cgactgcaga ttggtgctta cagcctgcgc gtcattgctc gccaatgcgg
     2221 ccaatgcgtc gcgttccatc accgcctgcc cgcgagtggt ttcgagtagc agatgcagat
     2281 ccagcaacac cggcatgcgc gcgcgcgtgg cgttattaac gccctggttg aaaccacgca
     2341 gttcattcag cgaaacggct gaaagtgcga acgttagaag aaacgtaact gtgaacgcca
     2401 gcgcgaggcg cgctcctatt ttcatgttgc tcaattgcat atcagagttt ccatctttcg
     2461 aatttcgcca cttggctgcc agcttcttgg gcaacgctgc ataacgacag gagagcggga
     2521 aacttaaggg tttttactta tggcgcggca aaaagtagcg gatccccgcg catgcgagcg
     2581 tcagcctccg agaaaacccg caagactgtg ctgtgggtag caataatcgg cgcggccgga
     2641 acggtagttg ccgccataat cgccatgggt catggacagg ctaccgaacg tctcttcatg
     2701 gccaactacc atctgacagg cgtggctagt cgccgtgtgc ggattgcgag tgctacagac
     2761 gtgagacatt agcaatatcc atcttatcta tttgcaacac tcgccggcct caactatgtt
     2821 gaagccggaa ggctcggctg ccgggcgggt gccatgtccg ctattcctcg cactcttacg
     2881 cgattccgag ttcgcgacaa tagcagggag actcggctat gacgacaccg ctactcggac
     2941 agatgatgga cgtgccgctg acggtttcct cactgctggc ccatgcgtca cgtcacttcg
     3001 gcacgaccga gatcgtgtca aaacgcattg aaggcgacgt gcatcgctac acatatcgcg
     3061 actgcgaaaa gcgcgcgaag cgactggcgc aggcgcttat cgcgctggat gtgcagccgg
     3121 gcgaacggat cggcacgctg gcatggaacg gttaccgcca tctggaagcc tattacggca
     3181 ccacaggctt cggggccgtc tgccacacca tcaacccccg gctcttcccc gagcagatcg
     3241 catggatcat caacgacgct aacgacagct acgtgctgtt cgatacgacg ttcgcgcaac
     3301 tgatcgattg ccttgcgccc ctctgcccgg aggtgcgcgg gtggatcgcg tttgccgacg
     3361 acgcgcatct gcctgcggtg cagagacccc taccgctcat cagctacgag acgttgctta
     3421 acccgcacga cggcgtattc gactggccgc tcctcgacga gcgcgtggca tcgtacctgt
     3481 gttacacctc gggcacgaca ggcaatccaa agggcgcgct gtattcgcat cgctccacgg
     3541 tgctgcacgc gtacgccgga tcgctgccgg atgcgatgtg tctatcggcg cgcgactcgg
     3601 tgttgcccgt cgtgccgatg ttccatgcga acgcatgggg cataccgcat gccgcgccgc
     3661 tcacgggcgc gaaactcgtc cttccgggca aggatctcga cggaaaatcg ctgttcgaac
     3721 tgatgcaggc ggaacgcgtc acgtattcgt ccggcgtgcc aaccgtctgg cttggcctgc
     3781 tcaactacat gaaggaagcg ggcgtgcggt tgtcgtcact gcaacgcgtg gcgatcggcg
     3841 ggtccgcgtg cccgccggcc atgctgcgcg ccttcgagga atacggcgtc gaagccgttc
     3901 acggatgggg catgaccgag atgtcaccgc tcggcacgct tgcaacactc aactgggaac
     3961 aatcacagcg gccgccggaa gagcagcgca ggctgcgcga aaagcagggg cacgcgatct
     4021 acggcgtgga catgaagatc gtcggcgccg atgggcgcga tctgccgtgg gacggcgtcg
     4081 cgttcggcga actgcacacg cgcggtccgt gggtgatcaa tcggtatttc cagaaggacg
     4141 actcgccgct cgtcgatggc tggtttccga ccggcgacgt ggccacgatc gaccgggacg
     4201 gtttcatgcg catcaccgac cgcagcaagg atgtgatcaa gtcgggaggg gagtggatca
     4261 gttcgatcga aatcgagaac atcgcgatcg cgcatccggc cgtcacagag gcggcctgca
     4321 tcgcctgcgc ccatccgaag tggctgcagc ggccgctgct catcgtcgtc aaacgcaagg
     4381 acgcggaggt cacgcgcgac gaactgctcg cgttctacga gggcaaggtc gcgaagtggt
     4441 ggatgccaga tgacgttgtc ttcgtcaacg aattgccgca tacggc
//
LOCUS       DATASH010001116         4947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5141431,
            whole genome shotgun sequence.
ACCESSION   DATASH010001116 DATASH010000000
VERSION     DATASH010001116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4947
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5141431"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="VHV99_29630"
     CDS             complement(<1..867)
                     /locus_tag="VHV99_29630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014895732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEX3639570.1"
                     /translation="MTEADPIAAYLSNLEHERRLSAHTLRGYTHELEELKLLAKGRPL
                     ESLTAVDIRGAVSRAHAGGLTARSISHRLSCWRAFYRWFAGRVDLPANPVATVRAPKQ
                     PKALPKALSVDDATRLMESPPPATPEGLRDHAMLELFYSSGLRLSELVGLDARFADAA
                     GYRSAGWLKLDSAEVEVLGKGNRRRIVPVGSKAIDALNAWLAVRDQMVRHDLHPLFLS
                     ARGNRLSPNVVRDRVKRAALVAGIPANVHPHVLRHSFATHVLQSSGDLRAVQELLGHA
                     SITATQVYTALDF"
     gene            complement(893..1612)
                     /locus_tag="VHV99_29635"
     CDS             complement(893..1612)
                     /locus_tag="VHV99_29635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HEX3639571.1"
                     /translation="MNDREVAEYLLATPEFFAEHAEMLATIRLANPHGKAAVSLQERQ
                     MEMLRDKNKHLERRLAELLRYGHENDSIASKFNRWTIRVMAERDPYALPRTISNGLRD
                     IFDVPQAALRVWDVAEPYAQAEFARHVGEEVRIFANSLTTPYCGANTGFEAAQWLVPA
                     VASVSGDGSAGATGESAHATESIALLALRDPEGKEEAPTFGLLVMGSADARRFHDGMA
                     TDFLTQIGALASAALSRLLPR"
     gene            complement(1849..2712)
                     /gene="dapF"
                     /locus_tag="VHV99_29640"
     CDS             complement(1849..2712)
                     /gene="dapF"
                     /locus_tag="VHV99_29640"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015001395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEX3639572.1"
                     /translation="MKLKFTKMHGAGNDFVVLDGYTQPVNLTPAQVRALADRHFGVGA
                     DQLLLVEKPTVDGVDFRYRIFNCDGGEVEHCGNGARCFVKFVRDSGLTDKHSVRVQVQ
                     NGTIVLTMQENGEVLVDMGTPVFEPERVPFATKGLEGRRDGADTLWPLDVNGVTRWIS
                     VVSMGNPHAVQVVDDVEDFPVLVEGPVIERHARFPQRVNAGFMQIVGRSEVKLRVYER
                     GAGETLACGTGACAAVAAGIRRGLLDAPVLVHTHGGKLTITWDSTQEGAALLMAGPAA
                     TVFEGEIELAE"
     gene            complement(2794..3678)
                     /locus_tag="VHV99_29645"
     CDS             complement(2794..3678)
                     /locus_tag="VHV99_29645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011850912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HEX3639573.1"
                     /translation="MLSRLGAHLAIGLLKFLALLPYGLVARMGDGLGWLLYQVPSNRK
                     RIVHINLSLCFPEWSAAHRAGIAQKHFRHAIRSYVERSVQWFGSAKKLEKLVQVDSEI
                     DLLDPDMPPTLLLGAHFVGIEAGSVFINHALRRQCGALYQPISNKVLDDVAKAARGRF
                     GADMASRADSARIVLRWLRDHKPVMLGADMDYGMRNSTFVPFFGVPACTLTAVGRLAG
                     VGHAQVVPFIGEVLPNYKGYRLKVFKPWANYPTGNDEVDARRMNAFLEEQIPLIPEQY
                     YWVHKRFKTRPPGEPSVY"
     gene            3957..>4947
                     /gene="metK"
                     /locus_tag="VHV99_29650"
     CDS             3957..>4947
                     /gene="metK"
                     /locus_tag="VHV99_29650"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006024140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEX3639574.1"
                     /translation="MANDYLFTSESVSEGHPDKVADQISDAILDAILTQDKYSRVAAE
                     TLCNTGLVVLAGEITTTANVDYIQVARNTIKRIGYDNTDYGIDYRGCAVLVAYDKQSP
                     DIAQGVDRAHDNNLDQGAGDQGLMFGYACEETPELMPLPIHLSHRLVERQANLRRDGR
                     LPWLRPDAKSQVTVRYVDGKPHSIDTVVLSTQHSPDIDLAVLREAVIEEVIKPTLPAN
                     LIKSDIKFLVNPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDR
                     SAAYAGRYVAKNIVAAGLASRCLIQVSYAIGVAQPTSVMVNTFGTGRVSDATIT"
BASE COUNT          898 a         1578 c         1570 g          901 t
ORIGIN      
        1 gaaatcgagc gcggtgtaga cctgcgtggc ggtgatgctg gcgtgcccga gcagttcctg
       61 cacggcccgc aggtcgccgc tcgactgcag cacgtgcgtg gcaaacgaat gccgcagcac
      121 gtgcgggtgc acattggcgg ggatgccggc gaccagcgcc gcgcgcttca cgcgatcacg
      181 cacgacattc ggcgacaagc gattgccgcg tgccgagaga aacagcggat gcagatcgtg
      241 tctgaccatc tggtcgcgca ccgcgagcca cgcgttcaag gcgtcgatcg ctttgctgcc
      301 gaccggcacg atgcggcgcc ggttgccttt gccgagcact tcgacttcgg cggagtccag
      361 cttgagccag cctgcggagc ggtagcccgc ggcatcggcg aaacgggcgt cgaggccgac
      421 cagttcggac agccgcaggc ccgacgagta gaacagttcg agcatcgcgt gatcgcgcaa
      481 accttcgggc gtcgcgggcg gcgggctttc catcaggcgc gtggcgtcgt cgacggaaag
      541 cgctttcggc agtgctttcg gctgtttcgg cgcccgtacg gtggcgaccg gattggccgg
      601 cagatcgaca cggcccgcga accagcggta gaacgcccgc cagcacgaca gccgatggct
      661 gatcgagcgc gccgtcaggc cgccggcatg cgcccgcgaa accgcgccgc gaatatcgac
      721 ggcggtgagg ctttcgagcg gccgcccttt ggccagcagc ttgagctctt cgagttcgtg
      781 ggtgtagccg cgcagcgtat gcgccgacag tcgccgctca tgttcgagat tcgacaggta
      841 ggcggcgatc gggtcggctt cggtcacggt ggccttatgt gggctcggcg gctcagcgcg
      901 gcagcagacg gctcagcgcc gcgctcgcca acgcgccgat ctgcgtcagg aaatcggttg
      961 ccatgccgtc gtgaaagcgg cgtgcgtcgg ccgaacccat caccagcagg ccgaaggtgg
     1021 gcgcttcttc cttgccttcg gggtcgcgca gcgcgagcaa cgcaatcgat tcggtggcgt
     1081 gcgcggactc gcccgtagcg cccgcggatc catcgccgct cacggatgcc accgccggca
     1141 ccagccactg cgctgcttcg aagccggtgt tcgcgccaca gtacggcgtg gtgaggctgt
     1201 tcgcgaagat tcgcacttcc tcgccgacat ggcgcgcgaa ctcggcctgt gcatacggct
     1261 cggcgacgtc ccaaacgcgc agcgcggctt gcggcacgtc gaaaatatcg cgcaaaccgt
     1321 tcgaaatcgt gcgcggcagc gcgtagggat cgcgttcggc catcacgcgg atggtccagc
     1381 gattgaattt cgaggcgatg ctgtcgtttt cgtgaccgta gcgcagcaac tcggccagac
     1441 gccgttcgag atgcttgttc ttgtcgcgca gcatttccat ctgccgctcc tgcagcgaca
     1501 ccgcggcttt gccgtgcggg ttcgcaagcc ggatcgtcgc gagcatttcc gcgtgttcgg
     1561 cgaagaattc gggggtggcg agcagatatt cggcgacttc gcgatcgttc atggtttcgg
     1621 ctaaaggact acgggacgac gccatcgggc gccgcgacgg tcatgagctc tgtcggccgg
     1681 tgaaaaagcc gcattgtccg gctcttcatc ggcttcttcg ttggcctatt cattgggcct
     1741 gttcaccagg ccattcctcg gcctgttcga gacctcgcac aggccacatg tgtggtactt
     1801 caccggccga tcgattggcc actgaatcgg acgtcagacc ggcgcgaatc actcggccag
     1861 ttcgatctcg ccttcgaaga cggttgccgc ggggccggcc atgagcagcg ctgcaccttc
     1921 ttgcgtgctg tcccacgtga tggtcagctt gccgccatgc gtgtgcacga gcacaggcgc
     1981 gtccagcagc ccgcggcgaa tgccggcggc gactgccgcg cacgcacccg tgccgcatgc
     2041 gagcgtttcg ccggctccgc gttcgtagac gcgcagtttg acttcgctac ggccgacgat
     2101 ctgcatgaag cccgcgttca ctcgctgcgg gaagcgtgca tgccgctcga tcaccgggcc
     2161 ttcgacgaga accggaaaat cctcgacgtc gtcgaccact tgcaccgcgt gcggattgcc
     2221 catcgacacc actgagatcc agcgcgtcac gccgttcaca tcgagtggcc agagcgtgtc
     2281 agcaccatca cggcggcctt cgaggccttt ggtggcgaac ggtacgcgct ccggttcgaa
     2341 cacgggcgtg cccatatcga ccagcacttc gccgttttcc tgcatggtca gcacaatggt
     2401 gccgttctgc acttgcacgc gcacgctgtg cttgtcggtc aggccgctgt cgcgcacgaa
     2461 cttgacgaag cagcgcgcgc cgttgccgca gtgctcgacc tcgccgccgt cgcaattgaa
     2521 gatgcgatat ctgaaatcga cgccgtccac agtgggcttc tcgaccagta gcaactggtc
     2581 ggcgccgacg ccgaaatgcc ggtccgcgag cgcgcgcacc tgcgccggtg tcaggttgac
     2641 cggttgggtg taaccgtcga gcacgacgaa gtcgttaccc gcgccgtgca ttttggtgaa
     2701 tttcagtttc atcgcgctat tgtaagtgac gcgctcgcag gtcggccgaa cgccgcttta
     2761 tttccgcctt cgggcgcttt tttcgaaggc cggtcagtag acgctcggct cgcctggcgg
     2821 ccgcgtcttg aagcgtttgt gcacccaata atactgttcc ggtatcaggg gaatctgctc
     2881 ctcgaggaag gcgttcatgc ggcgcgcgtc gacctcgtcg ttgccggtgg ggtagttcgc
     2941 ccatggcttg aacaccttga gacggtagcc cttgtaattc ggcagcacct cgccgatgaa
     3001 cggcaccact tgcgcatggc cgaccccggc gaggcgtccc acggctgtca gcgtgcacgc
     3061 aggtacgccg aagaacggca cgaacgtcga gttgcgcatg ccatagtcca tatcggcgcc
     3121 gagcatgacc ggcttgtggt cgcgcaacca gcgcagcacg atgcgcgcac tgtcggcgcg
     3181 gcttgccatg tccgcgccga agcggccgcg cgcggccttt gcaacatcgt cgagcacctt
     3241 gttggatatc ggctgataga gcgcgccgca ctgtcggcgc agcgcgtgat tgatgaacac
     3301 ggagccggcc tcgataccga cgaaatgcgc gccaagcaac agggtgggcg gcatgtcggg
     3361 atccagcagg tcgatctcgc tatccacttg cacgagcttt tcgagcttct tcgccgagcc
     3421 gaaccactgg acgctgcgct cgacataact gcggatcgcg tggcggaagt gtttctgcgc
     3481 gattcccgcg cggtgcgcgg cgctccactc cgggaaacac aagctcagat tgatgtggac
     3541 gatgcgctta cgattgcttg ggacctgata cagcagccag cccagcccat cgcccattcg
     3601 cgcaaccaga ccgtagggca acaacgccaa aaatttcagc agtccaatgg ccaggtgggc
     3661 accaagacga ctcaacatgc cgcccctccg tgtagggcgt cgagcgacgc tttgctcaag
     3721 aaacctgaag acgggtcgaa atacagcacg ggtgaacact ctgtgacaat caaaggaagt
     3781 cgctataata agggcttcgc cgagttaata gacaacttgc ggggcgaagc tggcgggtgc
     3841 gatccgtagg ataggccccc tgccagtaag gtaatccgct aaagcgtcgc cgcttcaagg
     3901 ctcccgaagc tggctacgtg aaactcaacc gtaaccacca aacaggagtc tgcaacgtgg
     3961 caaacgacta tctcttcacc tccgaatccg tttccgaagg ccatcccgac aaagtcgcgg
     4021 atcagatctc ggacgcaatc ctcgacgcca tcctgacgca agacaagtac tcgcgtgttg
     4081 cagccgaaac gctgtgcaac acgggtctgg tcgtgctggc cggtgaaatc accaccacgg
     4141 cgaacgtcga ttacattcag gtcgcgcgca acacgatcaa gcgtatcggt tacgacaaca
     4201 cggattacgg catcgactac cgtggctgcg ccgtattggt cgcctacgac aaacaatcgc
     4261 cggatatcgc ccaaggcgtg gaccgcgcgc acgacaacaa cctcgatcaa ggcgccggcg
     4321 accagggcct gatgttcggt tacgcgtgtg aagaaacgcc ggaactgatg ccgctgccga
     4381 tccacctgtc gcaccgtctg gtggaacgtc aggcgaatct gcgacgcgac ggccgtttgc
     4441 cgtggctgcg tccggatgcg aagtcgcagg tcaccgtgcg ttatgtcgac ggcaagccgc
     4501 attccatcga caccgtcgtg ctgtccacgc agcattcgcc agacatcgat ctggccgtgc
     4561 tgcgcgaagc cgtgatcgaa gaagtgatca agccgacgct gccggctaac ctgatcaaga
     4621 gcgacatcaa gttcctcgtg aacccgaccg gccggttcgt gattggcggt ccgcaaggcg
     4681 attgcggcct gacgggccgc aagatcatcg tcgacacgta cggcggcgcg gcaccgcacg
     4741 gcggcggcgc gttctcgggc aaggatccgt cgaaggtcga ccgctcggcc gcctatgccg
     4801 gccgttatgt cgcgaagaac atcgtggccg cgggtctggc gtcgcgttgc ctgattcagg
     4861 tgtcgtacgc gatcggcgtg gctcagccga catcggtgat ggtcaacacg ttcggcacgg
     4921 gccgcgtgtc ggatgcaacg atcacgc
//
LOCUS       DATASH010001117         1556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4941449,
            whole genome shotgun sequence.
ACCESSION   DATASH010001117 DATASH010000000
VERSION     DATASH010001117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4941449"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1103)
                     /locus_tag="VHV99_29655"
     CDS             complement(<1..1103)
                     /locus_tag="VHV99_29655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007587367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX3639575.1"
                     /translation="MSAVLAVEQHALRVEKLRLKGERAARARTMDVLDPYTNIRIGTV
                     PMASVDDVRDAFEYAANYRSTLTRYERSQILERAAALLRERTEEASDLISLESGLSKQ
                     DSRYEIGRVADVFKFAAMEALRDDGQSFSCDLTPHGKARRVFSQREPLAGVIVAITPF
                     NHPMNQVAHKLAPAIATNNRVILKPSEKVPLSAYYLADVLVEAGLPAPMLQVLTGDPR
                     EIAEELVTNRHADLVTFTGGVAIGKEIAAKAGYRRVVLELGGNDPLIVLDDADLDRAA
                     TLAVQGSYKNSGQRCTAVKRMLVQKRVAARFTELVVEKTLAWTYGDPFDPANQMGTVI
                     DEAAAKSFEARVNEAVSKGARLLCGNHRHGALYS"
     gene            complement(1100..>1556)
                     /locus_tag="VHV99_29660"
     CDS             complement(1100..>1556)
                     /locus_tag="VHV99_29660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007587366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphonoacetate hydrolase"
                     /protein_id="HEX3639576.1"
                     /translation="PDIPNARVILPITDPYVVHHGALGSFATIYLAPSVDAKALAARI
                     AELPGVDIVLDNKAACERFELPNDRVGDLVVVSGRNTVLGTRRAEHDLSGLTVPLRSH
                     GGVSEQEVPLLFNRPTDGFESKREGKGEGKRLRNFDVFNIALNHLELAS"
BASE COUNT          302 a          497 c          456 g          301 t
ORIGIN      
        1 gaatacaacg cgccatggcg atgattgccg cacagcaacc gcgctccctt cgagaccgct
       61 tcgttcacgc gtgcttcgaa tgatttggcc gcagcctcgt cgatcaccgt gcccatctgg
      121 ttggcaggat cgaagggatc gccgtaagtc cacgcaagcg ttttttccac cacgagctcg
      181 gtgaacctgg cggccacgcg cttttgcacc agcatgcgct tgaccgccgt gcaacgttgc
      241 ccggagttct tgtacgagcc ttgcacggca agcgttgccg cgcggtccag atcggcgtcg
      301 tccagcacga tcagtggatc attgccgccg agttccagca ccacgcgccg ataccccgcc
      361 ttcgccgcaa tctccttgcc aatagccacg ccaccggtaa aagtgacgag gtcggcatgc
      421 cggttggtga cgagttcttc ggctatctcg cgcgggtcgc ccgttaaaac ctgcagcatg
      481 ggcgcgggca aaccggcttc gaccaacacg tccgcgaggt aataagccga taacggcacc
      541 ttctccgagg gcttgaggat cacccgatta ttcgtcgcga tagccggcgc aagcttatgc
      601 gcgacctggt tcatcggatg attaaagggc gtgatcgcga cgatcacgcc ggccagcggt
      661 tcacgttgcg agaacacgcg acgggccttg ccgtgcgggg taaggtcgca tgaaaaactt
      721 tggccgtcgt cgcgcaacgc ttccatcgcg gcgaatttga acacgtcggc cacgcgtccg
      781 atttcataac gcgagtcttg cttcgacaaa cccgattcaa gcgaaataag atccgacgct
      841 tcttccgtgc gctcgcgcag caacgccgca gcgcgttcga gaatctgcga gcgttcatag
      901 cgcgtcagcg tcgagcggta attcgccgcg tactcgaatg cgtcgcgcac gtcgtcgacg
      961 cttgccattg gcacggtgcc aatgcgtatg ttcgtgtaag gatcgagaac atccatagtg
     1021 cgagcgcgcg ccgcacgctc gcccttcagg cgcagctttt cgactcgcag cgcatgttgc
     1081 tcaactgcaa gaacggcgct cacgatgcaa gctccagatg attcagcgcg atgttgaaga
     1141 catcgaagtt acgcaggcgc ttcccctcac ccttcccctc acgcttcgat tcgaaaccat
     1201 cggttggacg attgaacagc aagggcactt cctgctcgga cacgccgccg tgcgagcgca
     1261 acggtacggt caatcccgag aggtcgtgct ccgcgcgccg ggtgccgagt accgtgttgc
     1321 gtccgctcac gaccaccaga tctcccacgc gatcgttcgg caattcgaag cgctcgcacg
     1381 cagccttgtt gtcgagcacg atgtccacgc cgggcagttc ggcgatgcgc gccgccagcg
     1441 cttttgcatc gacggaaggc gccaggtaga tggtcgcgaa cgaaccgagc gctccgtgat
     1501 ggaccacgta gggatcggtg attggcagga tcacgcgcgc attcggaatg tccggc
//
LOCUS       DATASH010001118         2184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5142002,
            whole genome shotgun sequence.
ACCESSION   DATASH010001118 DATASH010000000
VERSION     DATASH010001118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5142002"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="VHV99_29665"
     CDS             <1..454
                     /locus_tag="VHV99_29665"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX3639577.1"
                     /translation="IKRAVQRANRTLCDAPEMREKISFGDYEIDFATRRLIRNGAEIA
                     LRSSEFALLKLFANNPMRLLTRPMVNSQLGRSENQNSSLDVAVCRLRTLIEDNPSRPK
                     LIQTLRGRGYMFVPSPKQDEESRVIVRDHDRRGCAASALHLLQTAGLA"
     gene            complement(475..843)
                     /locus_tag="VHV99_29670"
     CDS             complement(475..843)
                     /locus_tag="VHV99_29670"
                     /inference="COORDINATES: protein motif:HMM:NF022516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2866 domain-containing protein"
                     /protein_id="HEX3639578.1"
                     /translation="MQVSSNVSGAASRDFQSGRKTAAEAAIGRKPAKRVNAPADAGKN
                     VMKVRGCRISAPIVALWGEWCRIVEWINANGEYSCMAVPGDVTEAEIRQRVRTHRNGQ
                     RHTLTDDPGLPEQRVRLRRA"
     gene            1016..>2184
                     /locus_tag="VHV99_29675"
     CDS             1016..>2184
                     /locus_tag="VHV99_29675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX3639579.1"
                     /translation="MQSAPLHTQPVGRLHTSLSRARRGPLKMRFGITWKMFLAVLIAC
                     LVISLTMGYAVRVSFENGFLHYVRERDAGRIKAVIAKVTNEYATHGNWNFLRDHPDAW
                     LALLDAAIHDALREEIEAAQIGKRPSWQTFPGSGSVQQLLGPLTGPSEGPHWGMRFPP
                     APPAAAAIMRERDRPRASDSDSAYARPPLSRPYDGMSGSLPPGGPPVALFDSNHELVA
                     SSAREPAPDSAMKPVVYNGKIVGWLAINGPDTLSDAADIAFQAQQMRATWQIAGVAVV
                     LAAIVALLLARIVLAPVKRLMNATHSLAGGDFSARVPAGRRDELGRLASDFNILADSL
                     QQAERSRRNLIADISHELRTPLAVLRGELEAIEDGVHEFDRDSLKSLQTEVNMLN"
BASE COUNT          419 a          720 c          653 g          392 t
ORIGIN      
        1 gatcaagcgt gccgtgcaac gcgctaaccg aacgctgtgc gacgcccccg agatgcgcga
       61 gaaaatatcg ttcggcgact acgaaataga cttcgcgaca cggcgtctga tcaggaacgg
      121 cgctgaaatc gcgctgcgtt ccagcgagtt cgcgttactc aagcttttcg cgaacaaccc
      181 gatgcgtctg ctgacgcgcc cgatggtcaa tagtcagctg ggaagatccg agaaccagaa
      241 cagcagcctc gacgttgcgg tctgccgact gcgtactctg atcgaggaca acccgtcgcg
      301 cccgaagctt atccagacat tgcgcggacg cggctacatg ttcgtgccgt cgccgaagca
      361 ggacgaagaa agccgggtaa tcgtccgcga ccacgaccgc cgcggttgtg ccgcgtccgc
      421 actccatctc ctgcaaacag ctggcctcgc ttgaaatcga aaacgcggcg gcgctcacgc
      481 acgccgtaga cgcacccgct gctcaggcaa acccggatcg tccgtcaagg tgtgacgctg
      541 gccgttgcga tgcgtgcgca cccgctgccg gatctcggct tcggtcacgt cgcccggcac
      601 ggccatgcac gagtactcgc cgttcgcgtt gatccactcg acaatccggc accactcgcc
      661 ccacaacgcc acgatcggcg ccgaaatgcg gcagccgcgc actttcatca cgttcttgcc
      721 ggcatccgcc ggcgcgttga cgcgtttcgc gggctttctg ccaattgccg cttcggcggc
      781 cgtcttccgg ccagactgga aatcgcgtga agcggcgccg ctcacgttcg acgagacttg
      841 catggaaggt ccccctggtg aatgaagtgc cctttgccgc cttttttgcg gagttcatat
      901 cagggccgca cgatcgtacg tatcaatcgc gcagccacga cgaagaaaga atggagaagt
      961 tgtggagatt tcccggcaca tgtaacgcca tacgccaatc atctagaatt cgtccatgca
     1021 aagcgcccct cttcacaccc aacctgtcgg tcggctacac acgtcgttgt cgcgcgcacg
     1081 gcgtggcccg ctgaagatgc gcttcggcat cacctggaaa atgttccttg cagtcttgat
     1141 cgcctgcctc gtcatttcac tgacgatggg ctacgcggta cgcgtgagct tcgagaacgg
     1201 ctttttgcac tatgttcgcg agcgcgacgc agggcgcatc aaggctgtga tcgcaaaagt
     1261 gacgaatgaa tatgccacgc acggcaactg gaatttcctg cgtgatcatc cggatgcatg
     1321 gctcgcgctg ctggacgcgg cgatccatga tgccttacgc gaggaaatcg aggccgcgca
     1381 aatcggcaag aggccgagct ggcagacgtt tcccggcagc ggcagcgtgc agcaactgct
     1441 cgggccgttg actggcccat cggaaggtcc gcactggggc atgcggtttc cgccagcgcc
     1501 gccggcggcc gccgccatca tgagggagcg cgaccgccct cgcgcgagcg acagtgacag
     1561 cgcctatgca agaccgccgc tttcgcggcc gtacgatggc atgagcggta gccttccgcc
     1621 cggcggtccg ccggtcgcgc tgttcgactc caatcacgaa ctggtggcga gcagcgcacg
     1681 cgagcccgcg ccggacagcg ccatgaagcc cgtcgtctat aacggcaaga tagtcggctg
     1741 gctcgccatc aacggcccgg acaccctttc ggatgccgca gacattgcct tccaggcgca
     1801 gcagatgcgc gccacgtggc agatcgcggg cgttgccgtc gtgctcgcgg cgatcgtcgc
     1861 gttactgctc gcccgcatcg ttcttgcgcc agtcaagcgc ctcatgaacg cgacccatag
     1921 ccttgccggc ggcgacttca gcgcgcgggt tccggccggc cggcgcgatg aacttggccg
     1981 gctcgcgtcg gacttcaaca tactggcgga ttcgctgcaa caagccgaac gctcgcgacg
     2041 caacctgatc gcggacattt cgcacgagct gcgcacgccg cttgccgtgc tgcgcggcga
     2101 gctcgaagcg attgaagacg gcgtgcatga attcgatcgc gactcgctca agtccctgca
     2161 aaccgaagtc aatatgctca acaa
//
LOCUS       DATASH010001119         6281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_2963264,
            whole genome shotgun sequence.
ACCESSION   DATASH010001119 DATASH010000000
VERSION     DATASH010001119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 6281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6281
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2963264"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VHV99_29680"
     CDS             complement(<1..210)
                     /locus_tag="VHV99_29680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein"
                     /protein_id="HEX3639580.1"
                     /translation="MLICRMGAWRIESDGHLSAHELASFEGLRAVEAQRASEAAGERV
                     RLAQQARELKRRAWRHGHAAGKAAVL"
     gene            complement(204..797)
                     /locus_tag="VHV99_29685"
     CDS             complement(204..797)
                     /locus_tag="VHV99_29685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639581.1"
                     /translation="MKHTYTSAPADWVHDSWLESGRLIRHARHVRHERHARAAHAFLF
                     DRYPQLLQVSLAGLPPALPLWPRPACARLCRIVAALAFAGSLRRIVAAPARMAFAAGV
                     APHLLGTLQRHPRSGAADLSVEPIPSLFNRRDMTALGLALATRVAANSPYAFWWSLRL
                     PRDVVEAAACYRVQGLSALEAQALIADARRVMEGPAC"
     gene            complement(794..1711)
                     /gene="sctJ"
                     /locus_tag="VHV99_29690"
     CDS             complement(794..1711)
                     /gene="sctJ"
                     /locus_tag="VHV99_29690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion inner membrane ring
                     lipoprotein SctJ"
                     /protein_id="HEX3639582.1"
                     /translation="MRRALILLPVLLLLVGCRTSLFEGLDEDQANRIIAALSHHGING
                     YKERNADRTWNISVDDADAVVATELASAYALPRGGHANLGELFSRQGLISSPEEDRVR
                     YVYGLSQELSETLEKIDGVLLARVHIVLPEKDPMDPTQDTLPSASVMMRYRSDYNLEL
                     MRDRIRALVAGSVEGLTPARVSLTLLAVTPVLTFPGRCADGGDVASAAGGVAATDCLR
                     DGGNPSRTTAMLGVLALLVLLTAGFMWLWRSQTRTWFGRKTRGDAARAEPGQRAQAAR
                     QAPRDGSEAGTAESAAGASKSANRTGGDA"
     gene            complement(1711..2052)
                     /locus_tag="VHV99_29695"
     CDS             complement(1711..2052)
                     /locus_tag="VHV99_29695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX3639583.1"
                     /translation="MLQVPVTTLPAQSGALSPQSSVSPTPGATVRVADGTADQGSLIE
                     RLLARAQDVQDTSAQQLSQHLDQLDAGRISTSDMLRLQADMGAFSVRVQITVRVADET
                     GRAIQTLSQRS"
     gene            complement(2054..2512)
                     /locus_tag="VHV99_29700"
     CDS             complement(2054..2512)
                     /locus_tag="VHV99_29700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639584.1"
                     /translation="MQSDTLELLISIGHYARVHGRFDEARYLFERLARLYPQRAFPYL
                     GLGLVEIDRANYRAASLLFGRAVEVAPDSALARAWLGVCQVFEGHYARGARTLSAVAD
                     GDEPGASSMAAAFLNLPECLPYRHAARATQAERAVGPISTIERLTIRGSH"
     gene            complement(2579..2899)
                     /locus_tag="VHV99_29705"
     CDS             complement(2579..2899)
                     /locus_tag="VHV99_29705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639585.1"
                     /translation="MNQVNTTMSPNLGGNFAGATGGSTVGANGGLSSQKVAEVLAKIL
                     EKQNQDFDTKLQQAEGTDEKQQNAAMMKVQQAMGAVNTTQSVATACVQGLTDAQKETA
                     RASH"
     gene            complement(2912..3175)
                     /locus_tag="VHV99_29710"
     CDS             complement(2912..3175)
                     /locus_tag="VHV99_29710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639586.1"
                     /translation="MLEKTAGKSTLGACADLPLEAQLAEAGGAARESIVARLTTEIDA
                     MNSALPGASPTVRKELILLVECLRAARQVVERSASFTNARDKK"
     gene            complement(3153..4253)
                     /locus_tag="VHV99_29715"
     CDS             complement(3153..4253)
                     /locus_tag="VHV99_29715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX3639587.1"
                     /translation="MTADAQIVVMDGVHTGASVVLSPDKPLRIGSGGEADLMVIDEGV
                     EPLHATVELHGAGLALVADGPHVAVFGRPVAPGRRVLLERGAWFSAGAVTFQFGGRDA
                     PGATMARDAERAYLRRHAPLAYIARHWTDASPVTRAVAVAVPVAFALLTWTASSQWSS
                     DVPRAVRANDAFRLVKTHLDPKSGALIYEGYVESAADLAALTASAWSRQRAPVMHVFV
                     LAQLREQVGEFLARYYRGAAVHATQPGAFAATLPDAHGFLSPESWDYARVARLAGAEI
                     SGLRGLTFPGHAQQGERVRVPLEALGLNLLASRHAFWLTDAQGVRYFVGARLPIGRVT
                     RISACAAELTRDDDGSVYEFFLDAAHAGKNCR"
     gene            complement(4243..>6281)
                     /gene="sctC"
                     /locus_tag="VHV99_29720"
     CDS             complement(4243..>6281)
                     /gene="sctC"
                     /locus_tag="VHV99_29720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system outer membrane ring
                     subunit SctC"
                     /protein_id="HEX3639588.1"
                     /translation="RHVAGPLGLASAVLLLPAAHAATPVWQGAPIHYAVNGAPLPDVL
                     RDVLAVEGLSADIGRDVKGLVNGRFDDTPGNVFTQLIEAYGLVWYFDGKAMHVASASD
                     VRSRVIPFAPMTREAVASLLRNLDLDDARLPIKYSATTVKVAGPSKFVDAVAEAIDKA
                     QRQTTVAPSFDETVIRVFPLRYAQAQDVRYAVGSQEQVVPGVASLLRRLMTDTWQATA
                     QRASRSRADARADARNARGGPPPLPSLLGLGLAGTPSESAAEAAPLPDSESDTAQPAA
                     TPARRNIVADQRSNSVVISDLVSMMPNYERAIAMLDQPQELVEIDAVVIDVSSNAARS
                     LGVRWGGANGRINVASGTASAPTSFRAVDLVTPASAAAGLNLATLIGNSAQYLFAQIH
                     ALEEAGQARVLSRPQVLTLNNTEAALTSRSTVYVRVAGNQAVDLFNIDTGMTLKVTPS
                     VESSGEPRRNIRLNIQIEDGTFNTSASVDGIPKVDNHSVVTQAIVRDGESLLIGGYQY
                     ERSESTTSKVPLLGDVPYLGTLFRDTQTTRERLERLVLITPRLKRLAGDGASNAAQGA
                     GGTTMDIASSSPDTPSVGAVVASALPVPTMLPASDAPVAARPRPRQQAQQAQQAQQAQ
                     QAQQPPAPDVPSAIDAALRALAQPAVPQPSASSALSRPGLPLVSKMPWENRHDR"
BASE COUNT         1025 a         2153 c         2097 g         1006 t
ORIGIN      
        1 cagcaccgcc gccttgccgg ccgcgtgccc atgacgccac gctcggcgct tgagttcgcg
       61 cgcctgctgg gcgaggcgga cgcgctcacc tgccgcttcg ctcgcgcgtt gcgcctcgac
      121 cgcgcgcaag ccctcgaacg acgccagctc atgcgcactc agatggccgt cggattcaat
      181 tcgccatgcg cccattcgac agatcagcat gctggcccct ccatcacacg ccgcgcatcg
      241 gcgatcagcg cctgggcttc aagcgctgaa aggccctgca cgcgatagca cgcggcggcc
      301 tccacgacgt ctcgcggcag gcgcaagctc caccagaacg cataggggga gttcgcggcg
      361 acgcgggttg ccagcgccaa cccgagtgcc gtcatgtcgc gccggttgaa cagcgacggt
      421 atcggctcga cgctcaggtc ggccgccccg ctccgcgggt gccgttgcag cgtgccgagc
      481 agatgcggcg cgaccccggc cgcgaacgcc atgcgggccg gcgcggcaac gatgcggcgc
      541 aacgagccgg cgaatgcaag cgcggcgacg atccggcaca ggcgcgcgca cgccggccgt
      601 ggccacagcg gcagcgccgg cggcaagccg gcgaggctca cctggagcaa ttgcgggtaa
      661 cggtcgaaca gaaaagcatg cgcggctcgc gcgtgccgtt cgtgacgaac gtgacgagcg
      721 tgacgaatga gacgccccga ttcgagccaa ctgtcatgca cccaatcagc gggcgcgctg
      781 gtgtatgtgt gtttcatgcg tcacctcccg tacggttcgc ggacttcgag gccccggcgg
      841 cgctctcggc ggtgccggct tccgagccgt cgcgcggcgc ctggcgggca gcctgcgcgc
      901 gttgtcccgg ctcggcccgc gctgcgtcgc cgcgcgtctt ccgtccgaac cacgtgcgcg
      961 tttgcgaacg ccacaaccac atgaatccgg cggtcagcag cacgaggaga gccagcacac
     1021 ctagcatcgc cgtggtgcgc gacgggttgc cgccgtcgcg caggcaatcc gtggcggcga
     1081 ccccacccgc ggcactggcg acatcgccac cgtccgcgca tcggccggga aaggtcagca
     1141 ccggcgtgac cgccagcagc gtcagcgaca cgcgcgcggg cgtcagccct tcgacactcc
     1201 ccgccaccag cgcgcgaatc ctgtcgcgca tcagctcgag gttgtagtcg ctgcggtagc
     1261 gcatcatcac ggacgccgag ggcagcgtgt cctgggtcgg atccatcgga tctttttcgg
     1321 ggagcacgat gtgcacgcgg gcaagcagca cgccatcgat cttttcgagc gtttcggaca
     1381 actcctggga gagcccgtac acatagcgga cacggtcctc ctcggggctc gatatcaacc
     1441 cctggcggct gaacagctcg ccgagattcg catggccgcc gcgcggcagc gcgtaggcgc
     1501 tggccagttc ggtggccacg accgcgtccg catcgtcgac cgaaatgttc caggtgcggt
     1561 ccgcgttacg ctccttgtaa ccgttaatgc cgtgatggct gagcgcggcg atgatgcggt
     1621 tcgcctggtc ctcgtccagg ccttcgaata gcgaggttct acagccgacg aggagcagca
     1681 gcaccggcag caggatcagg gcgcgccgca tcagcttcgc tgcgacagcg tctggatggc
     1741 gcggccggtc tcgtcggcaa cgcgcaccgt gatttgcacc cgcaccgaga aagcgcccat
     1801 gtcggcctgc aggcgcagca tgtcggaggt cgagatgcgg ccggcgtcga gctggtcgag
     1861 gtgctgcgaa agctgctgcg ccgacgtgtc ctgcacgtct tgcgcgcgcg ccagaagacg
     1921 ctcgatcagc gagccctggt cggctgtccc atcggcgacc cgtacggtgg cgccaggcgt
     1981 gggcgatacg gaagattgcg gggacaatgc gccggactgc gccggcaagg ttgtgacagg
     2041 gacttgcaac atatcaatgg ctgcctcgaa tagtcagtcg ttcaatggtt gaaatggggc
     2101 ccacagcgcg ctcggcctgc gtggcacgag cggcgtggcg ataaggcaag cattcaggca
     2161 gattcaggaa cgccgcggcc atcgaactcg cgccgggctc gtcgccgtcc gccaccgcgc
     2221 tcagcgttcg tgcaccgcgc gcgtagtggc cttcgaacac ctggcacacg ccgagccatg
     2281 cacgcgccag cgcactgtcg ggcgcgactt ccaccgcgcg gccaaacagc agggacgcgg
     2341 cgcggtaatt cgcccggtcg atctcgacca gccccagccc gagatacgga aaggcccgct
     2401 gcggatacag cctggcaagc cgctcgaaca gatagcgcgc ctcatcgaag cgcccgtgca
     2461 cgcgggcgta gtgaccgatc gaaatcagca gctccaaggt gtcgctctgc atcggcgatg
     2521 gctccccgaa gacgccgcaa ggcgcctccg tcctggtcaa agtgaaacgg gacatgtgtt
     2581 aatgagaggc gcgcgccgtt tccttctgcg cgtcggtgag accctgcacg catgccgtcg
     2641 caacgctctg ggtcgtgttc accgcgccca ttgcctgctg cactttcatc atggcggcgt
     2701 tctgctgctt ttcgtcggtg ccttctgcct gctgcaactt ggtatcgaag tcctggttct
     2761 gtttctcgag gatcttcgcc agcacttcgg ccaccttctg ggacgacagg ccgccgttcg
     2821 cgccgaccgt cgagccgccc gtggcaccgg cgaagttgcc gcccagattc ggggacatcg
     2881 tcgtattgac ctgattcatg atttccactc cttacttttt atcgcgcgcg tttgtgaagc
     2941 tggcgcttcg ttccaccact tgccgagccg cgcgcaaaca ctcgacaagc agaatcagtt
     3001 cctttcgcac cgttggcgac gcgcccggca gcgcgctgtt catggcgtct atctctgtgg
     3061 tcaggcgtgc cacgatcgac tcgcgcgccg cgccgccggc ttcggcaagt tgcgcttcga
     3121 gcggaaggtc ggcgcacgcg ccgagcgtgc ttttaccggc agttttttcc agcatgtgcg
     3181 gcatccagaa agaattcgta gaccgaaccg tcgtcgtcac gcgtgagctc cgcggcgcaa
     3241 gccgagatgc gcgtcacccg gccgatcggc agtcgcgcgc cgacgaaata gcgcaccccc
     3301 tgcgcgtccg tgagccagaa cgcgtgacgg ctggcgagca gattcaggcc gagtgcctcg
     3361 agcggtaccc gcacccgctc gccctgctgg gcatgaccgg gaaaagtgag accgcgcagt
     3421 ccgctgattt cggcgccggc gagacgcgcg acacgtgcgt agtcccacga ctcgggcgac
     3481 agaaagccgt gcgcgtcggg tagcgtcgcg gcgaatgcac cgggttgcgt ggcgtgcact
     3541 gcggcgccgc ggtagtagcg cgcgagaaat tcgccgactt gttcacgcag ttgcgcgagc
     3601 acgaacacat gcatcaccgg cgcgcgctgc cgcgaccagg cgctggcggt cagcgccgcc
     3661 agatcggcag ccgactcgac gtagccctcg tagatgagtg cgccggactt cgggtcgagg
     3721 tgcgtcttca ccagccggaa cgcgtcgttc gcacgaacgg cgcgcggcac gtcgctgctc
     3781 cactggctgg aagcagtcca cgtgagcagc gcaaatgcaa ccggcaccgc aacggctacg
     3841 gcccgcgtca ccggcgacgc atcggtccag tgccgcgcga tatacgccag cggcgcatga
     3901 cggcgcaggt aggcacgctc ggcgtcgcgc gccatcgtcg cgcctggcgc atcgcggccg
     3961 ccgaactgga acgtgacggc ccctgccgaa aaccacgcgc cgcgctcaag gagcacgcgc
     4021 cgcccgggcg ctacagggcg gccgaacacg gcgacgtgcg gaccgtcggc gaccagcgcg
     4081 agccctgccc cgtgcaactc gacggtggcg tgcagcggtt cgacgccttc gtcgatcacc
     4141 atcaggtcgg cttcgccgcc gctacctatg cgcaacggct tgtcgggcga cagcaccacg
     4201 cttgcgccgg tgtgcacgcc gtccatcacg acgatctgtg catcagcggt catggcggtt
     4261 ctcccatggc atcttgctca ccagaggcaa tcccggccgt gacagggccg atgacgcaga
     4321 cggctgcgga acggcgggct gggccagcgc ccgtaatgcc gcgtcgatcg ctgatggcac
     4381 atcgggggca ggcggttgtt gtgcctgctg tgcctgctgc gcttgctgcg cttgctgcgc
     4441 ttgttgccgt ggccgtggac gcgcagcaac aggcgcgtcg ctcgccggta gcatcgtcgg
     4501 tacgggcaac gcgctcgcca ccaccgctcc aaccgaaggc gtatccggac ttgaactggc
     4561 gatatccatc gtcgttccac cagcgccctg cgccgcattc gacgccccat cacccgccag
     4621 gcgcttcagg cgcggcgtga tcaggaccag ccgttcgagg cgctcgcgcg tggtctgggt
     4681 atcgcgaaac aacgtgccta gataaggcac gtcgccgagc agcggcacct tggacgtggt
     4741 gctttcgctg cgttcatatt gatagccgcc aatcagcaga ctctcgccat cccgcactat
     4801 cgcctgagtg acgaccgagt ggttgtcgac cttcgggata ccgtccaccg atgccgacgt
     4861 gttgaacgta ccgtcttcga tctggatgtt gagccggata ttgcggcgcg gctcgccgct
     4921 agattcgacg ctcggcgtca ccttcagcgt catccccgta tcgatgttga agaggtcgac
     4981 cgcctggttg cccgcgaccc gcacatagac cgtgctgcgc gaagtcagcg cggcttccgt
     5041 gttgttcagc gtcaagacct gcgggcgcga cagcacacgc gcctggccgg cctcctccag
     5101 cgcatgaatc tgggcgaaca ggtattgcgc cgagttgccg atcagcgtcg caagattgag
     5161 tccggccgcc gcgcttgccg gcgtgaccag gtccaccgca cggaagctcg tcggcgcgct
     5221 cgcggtcccg gaggcgacgt tgatacgccc gttcgcgccg ccccagcgca cgccgagcga
     5281 gcgcgccgcg ttcgacgaga cgtcgatcac caccgcgtcg atctcgacga gctcctgcgg
     5341 ctggtcgagc atcgcgatcg cgcgctcgta gttcggcatc atcgacacga ggtcgctgat
     5401 gaccaccgag ttactgcgct gatcggcgac gatgttgcgc cgcgcgggtg tcgcggcggg
     5461 ctgtgcggtg tcggactcgg aatcgggcag cggggccgcc tcggcggcgc tctcagacgg
     5521 ggtgccggca aggccgaggc cgagcaggct cggcaacggc ggcgggccgc cgcgcgcatt
     5581 gcgagcatcg gcgcgagcgt cggcgcgcga gcgggaggca cgctgggccg tggcctgcca
     5641 ggtgtccgtc atcaagcggc gcaacagcga agcgacgccc ggcacgacct gctcctgcga
     5701 cccgaccgcg taacggacgt cctgcgcctg cgcataacgc agcgggaaaa cgcggatgac
     5761 ggtctcgtcg aacgacggcg cgacggtggt ctgccgctgg gccttgtcga tcgcctcggc
     5821 caccgcatcg acgaatttcg acgggcccgc taccttgacg gtggtcgcgg aatatttgat
     5881 cggcaggcgg gcgtcgtcga gatcgaggtt gcgcagcagg gacgcgacgg cctcgcgggt
     5941 catcggcgcg aacgggataa cccggctgcg cacatccgac gcgctcgcga catgcattgc
     6001 cttgccgtcg aaataccaga cgagtccgta tgcctcgatc agctgggtga agacattgcc
     6061 cggtgtgtcg tcgaagcggc cattgaccag ccccttcacg tcgcggccga tgtcggcgct
     6121 caagccttcg acggcgagca cgtcgcgcaa gacatcgggc aacggggcgc cgttcactgc
     6181 gtagtgaatc ggcgcacctt gccatacggg tgtcgcggca tgcgccgcgg gcaggagcag
     6241 cacggccgag gcgagcccga gcggacccgc gacatgacgc a
//
LOCUS       DATASH010001120        11124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4941781,
            whole genome shotgun sequence.
ACCESSION   DATASH010001120 DATASH010000000
VERSION     DATASH010001120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 11124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11124
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4941781"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /locus_tag="VHV99_29725"
     CDS             complement(<1..970)
                     /locus_tag="VHV99_29725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX3639589.1"
                     /translation="MQFDLLHIGSLVALAHILGVVAACHAILNTRTSQGAIAWAVSLV
                     AMPYLTLVPYLFLGRSKFQGYADARRVENELLRTRAHPPEWDTQASSAGLPTLELGGR
                     LVHSLTRLGGMPFLPGNAVRTLVNGAATFEAIFEAIEGARKYVIVQFFIVRDDALGEM
                     LKDALIAKAQQGVRVYFLYDSIGSFDLPHRYVAALRAAGVETHPFATNRRFVNRLQLN
                     FRNHRKIVSVDGERAFVGGHNVGVEYLGGKPPLSPWRDTHIEVRGPAVASIQFVFTED
                     WHWATQQLPEFDVPCAAQSHQPNHRSDDEGMHCLVVPSGPADKQETC"
     gene            complement(1000..1884)
                     /gene="prmB"
                     /locus_tag="VHV99_29730"
     CDS             complement(1000..1884)
                     /gene="prmB"
                     /locus_tag="VHV99_29730"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018435507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX3639590.1"
                     /translation="MTLPFSTVRDLLRFAVSRFNQAELSFGHGSANAYDEAAYLILHT
                     LHLPLDLLEPFLDARLSASEIDAVLNVIERRAGQRVPAAYITQEAWMHGFRFHVDERV
                     IVPRSFIGELLQDGLQPYVEDPEQVSAVLELCTGSGCLAILAAHAFPNADIDAVDLSA
                     PALEVATRNVADYRLDDRVALFEGDLYAPLAERRYDVIISNPPYVNATSMQELPAEYK
                     HEPDMALAGGADGMDIVRRIIAEARNWLTDNGVLVVEIGNERANVEAAFGGLDLVWLS
                     TSAGDDNVFLIQAADLPV"
     gene            complement(1904..3043)
                     /gene="dapE"
                     /locus_tag="VHV99_29735"
     CDS             complement(1904..3043)
                     /gene="dapE"
                     /locus_tag="VHV99_29735"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006493370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEX3639591.1"
                     /translation="MSGTLALTEQLIARASVTPNDQHCQRLLIERLSALGFEHETIES
                     HGVTNLWAVKRGVDGTAGKLLAFAGHTDVVPTGPLEQWHSAPFEPTHRDGKLYGRGAA
                     DMKTSIAGFVVASEEFVTAHPAHRGSIAFLITSDEEGPATDGTVKVVEALQARGERMD
                     YCIVGEPTSSAQFGDMVKNGRRGSMSGKLIVKGVQGHIAYPHLAKNPVHLLAPALAEL
                     VAERWDDGNEYFPPTTWQVSNLHAGTGATNVIAGHADVMFNFRFSTASTVEGLQARVH
                     AILDKHQLEYDLQWTVSGLPFLTPRGDLSNALAKAIKDETGVTTELSTTGGTSDGRFI
                     ARICEQVIEFGPLNASIHKIDEHIEVAHIEPLKNVYRRVLEQLIA"
     gene            complement(3241..3606)
                     /locus_tag="VHV99_29740"
     CDS             complement(3241..3606)
                     /locus_tag="VHV99_29740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018435509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HEX3639592.1"
                     /translation="MARGTKTVVYGIPNCDTVKKARVWLEEHGVEFEFHDFKKHGVTE
                     PLVQDWLKDVKLDALLNRRGTTWRGLSDDMKAAADTQSGAIALMIHKPSVIKRPVLVV
                     NGKVKSLGFSVDEYTALFA"
     gene            complement(3606..4433)
                     /gene="dapD"
                     /locus_tag="VHV99_29745"
     CDS             complement(3606..4433)
                     /gene="dapD"
                     /locus_tag="VHV99_29745"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007580558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEX3639593.1"
                     /translation="MSQQLQQIIDTAWENRADLSPKAAPAEVREAVAHAIEQLDKGTL
                     RVAEKKDGDWIVNQWLKKAVLLSFRLEDNAPMPAGGYSQFYDKVPSKFANYTAEDFAA
                     GGFRVVPPAIARRGSFIAKNVVLMPSYTNIGAYVDEGTMVDTWATVGSCAQIGKNVHL
                     SGGVGIGGVLEPLQANPVIIEDNCFIGARSEVVEGVIVEENSVISMGVYLGQSTKIYD
                     RETGEVSYGRIPAGSVVVAGNLPSKDGSHSLYCAVIVKKVDAKTRAKVGLNELLRGD"
     gene            complement(4516..5757)
                     /gene="dapC"
                     /locus_tag="VHV99_29750"
     CDS             complement(4516..5757)
                     /gene="dapC"
                     /locus_tag="VHV99_29750"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HEX3639594.1"
                     /translation="MNPLLDSLHPYPFEKLRALFKDVTPPAGLAHISFGIGEPKHPTP
                     ALIRDAVIASLGGLSAYPATIGSPALRESIATWVTQRYNLPPVDPATQVLPVSGSREA
                     LFALAQTVIDPKQDTARNNARHADGEPAIVLCPNQFYQIYEGATILAGAQPYFVNSDP
                     ARNFACDYAAVPADIWARTQLLYVCSPGNPTGAVLTLDDWRELFALSDRYGFVIASDE
                     CYSEIYFDETNPPLGGLEAAHKLGRGFERLVMLSSLSKRSNVPGMRSGFVAGDAAILK
                     DFLLYRTYHGAALSTVFQNASIAAWNDETHVRENRAKYVQKFATVTPMLAEVLDVRLP
                     DAAFYLWANVSRTGLTDTEFAQRLYADYNVTVLPGSFLARTAHGVNPGRNFVRMALVA
                     DVDECTQGAQRIVDFCRALAG"
     gene            6207..7193
                     /locus_tag="VHV99_29755"
     CDS             6207..7193
                     /locus_tag="VHV99_29755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX3639595.1"
                     /translation="MTHWLRNGWPTLAIMLGASVWGVIWYPLRMLNALGVTGTAASAL
                     TSGAGCLFVLLVRHRALKTVRWHWLLPALALAAGITNLGFVWGSIHGEVMRVLLLFYL
                     TPAWTALFAHFILHERLTWAGAGLAALSLAGAMTMLWSPQLGLPVPGNLAEWAGLAGG
                     MGFAMSNVLILKTSRVLPEMKPEMRTATIFGGAAVFSALASLFEAMPAPPTGAHLNTA
                     ALLVLGLGFLLASNNMLVQYGLSRVPANRASIIMLFEIVVTALSAWLFAGETPGPREW
                     AGGACIVLASVLSSWVHRTRALPPDPANQDAGLNAAEGTDRKDGKNRSRAMV"
     gene            7343..10861
                     /gene="smc"
                     /locus_tag="VHV99_29760"
     CDS             7343..10861
                     /gene="smc"
                     /locus_tag="VHV99_29760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013698522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEX3639596.1"
                     /translation="MRLTSIKLAGFKSFVDPTHFQVPGQLVGVVGPNGCGKSNIIDAV
                     RWVLGESRASELRGESMQDVIFNGSTARKPGSRASVELVFDNADGRAAGQWGQYAEIA
                     VKRVLTRDGTSSYYINNLPARRRDIQDIFLGTGLGPRAYAIIGQGMIARLIEAKPEEL
                     RVFLEEAAGVSKYKERRRETENRLHDTRENLIRVEDIVRELSANLEKLEAQAIVATRY
                     KDLQADGEEKQRLLWLLRKNEAGGEQERQQRAIEQAQIDLEAQTAKLREVEAQLETLR
                     VAHYSASDAMQGAQGALYEANSEVSRLEAEIRFIVESRNRVQAQIAALTAQREQWQSQ
                     AQKAQDDLEDAEEQLAVAEEKAALAEDEAAAKHDAMPALEARWRDAQTELNAERGGIA
                     QTEQALKLEAAHQRNADQQLQQLQQRHERLKSEAGGLDAPDEAQLEELRMQLAEHEEI
                     LHDAQTRLADAQETLPRLDGERRSAQERVQSESAQIHQLDARLAALKQLQENVQTEGK
                     IQPWLEKHELNGLPRLWKKLHVEAGWEAALEAVLRERLAALEVSNLDWVKAFATDAPP
                     AKLAFYAPPAAGQPVVTPPALRPLLSLVRIDDAGIRAVLNDWLGLAFVADDLQQALGM
                     RAQLPEGGSFVVKAGHVVTRVGVQLYAADSEQAGMLARQQEIENLTRQVRAQALLADE
                     AKAAAIRAEAAHTQAAQALTDVRQQSERATQRVHALQMDVLKLAQAHERYTQRSTQIR
                     EELAEITAQIEEQRALRGESEANFERHDSELAELQARFEDHQMAFEALDEALTSARTQ
                     SRDLDRAATDARFAARNMANRIDELKRSIQVSHEQSERVAGSLEDARAELETINEQTA
                     HTGLQDALDIRAVKEEALHATRLELDDLTAKLRAADETRLTAERAQQPLRERINELQL
                     KEQAARLNGEQFIEQLAAAGVDEAELQAKLTPDMKPSYLQGEVTRINNAITALGPVNM
                     AALDELKAATERKTFLDSQSGDLNNAIETLEDAIRKIDGETRTLLQGTFDEVNRHFGE
                     LFPRLFGGGQARLIMTGDEILDAGVQVMAQPPGKKNSTIHLLSGGEKALTATALVFAM
                     FQLNPAPFCLLDEVDAPLDDANTERFANLVRAMSDKTQFLFISHNKIAMEMAQQLIGV
                     TMQEQGVSRIVAVDMETAAGFAQNIG"
     gene            10979..>11124
                     /locus_tag="VHV99_29765"
     CDS             10979..>11124
                     /locus_tag="VHV99_29765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018435513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX3639597.1"
                     /translation="MDELTLGLIGAGAVVVGGVVVYNAWQGAKVRRKMPRPMPADTAE
                     SFAR"
BASE COUNT         2092 a         3478 c         3668 g         1886 t
ORIGIN      
        1 aacaggtctc ctgcttgtcg gcggggccgc tcggcaccac gaggcagtgc atgccctcat
       61 catcggagcg atggttcggc tgatgactct gcgcggcgca cggcacgtcg aactcgggca
      121 actgctgggt cgcccagtgc cagtcctcgg tgaacacgaa ctggatgctg gcgacggccg
      181 ggccgcgcac ttcgatatgc gtatcgcgcc atggcgacag aggtggtttg ccgccgagat
      241 attcgacacc gacgttgtgg ccgcccacaa acgcgcgttc gccatccacc gagacgatct
      301 tgcggtgatt gcgaaagttc agctgcaggc ggttgacgaa gcggcgattg gtggcgaacg
      361 ggtgggtctc cacgccggcc gcccgcagcg ccgccacgta acggtgtggc agatcgaaac
      421 tgccgatgct gtcgtacagg aagtagacgc gcacgccttg ctgcgctttc gcgatcagcg
      481 cgtctttgag catttcgccg agcgcgtcgt cgcgcacgat gaagaactgc acgatcacgt
      541 atttgcgcgc gccttcgatc gcttcgaaga tcgcctcgaa agtcgcggcg ccgttcacca
      601 gcgtgcgcac ggcattgccc ggcaggaacg gcatgccacc caggcgcgtg agcgaatgca
      661 cgagcctccc gcccagctcc agcgtaggca gccccgcgga ggaagcctgc gtgtcccatt
      721 ccggaggatg ggcgcgcgtc cgcagcaatt cgttttcgac gcggcgagcg tcggcgtagc
      781 cctggaattt gctgcggccg aggaacaggt acggcaccag cgtcagatac ggcatggcga
      841 ccagcgacac ggcccacgcg atcgcgcctt gcgatgtgcg cgtgttcaga atcgcgtggc
      901 acgccgcaac cacgccgaga atatgggcga gggcgacgag cgagccgata tgaagcaggt
      961 cgaattgcat aggcggtccg gcgatccggc gataaaacgt cagaccggca gatccgcggc
     1021 ctgaatcagg aacacgttgt catcgccggc gcttgtcgac agccagacca gatcgaggcc
     1081 gccgaacgcc gcttcgacgt tcgcgcgctc gttgccgatc tcgaccacca gcacgccgtt
     1141 gtcggtcagc cagttgcgcg cctcggcaat gatccggcgc acaatgtcca tgccgtccgc
     1201 gccgcctgcc aaagccatgt ccggctcgtg cttgtattcg gcgggcagtt cctgcatcga
     1261 ggtggcgttc acatacggcg gattgctgat gatcacgtca tagcggcgct cggcgagcgg
     1321 cgcgtacaga tcgccttcga agagggcgac acggtcgtcg agccggtagt ccgcgacgtt
     1381 gcgcgtggcg acttccagag cgggcgcgga aaggtcgact gcgtcgatat ccgcgttcgg
     1441 gaatgcgtgc gccgcgagga tcgcaaggca gccggagccg gtgcagagtt cgagcacggc
     1501 gctcacctgt tcgggatctt cgacgtacgg ctgcaagccg tcctgcagca actcgccgat
     1561 gaacgagcgc ggcacgatca cgcgttcatc cacgtgaaag cggaagccgt gcatccacgc
     1621 ttcctgcgtg atgtatgcgg ccggcacgcg ctgcccggca cggcgctcga tcacattcag
     1681 cacggcgtcg atttcagagg cggagagacg cgcgtcgaga aacggctcca gcagatcgag
     1741 cggcagatgc agcgtgtgca gtatcagata ggcggcttcg tcgtaggcgt tggccgagcc
     1801 gtggccgaac gacaactcgg cctggttgaa gcgcgacacc gcgaaacgca gcaggtcgcg
     1861 gacggtggag aacgggagcg tcatcgcgga aattccttgg gggtcaggcg atcaattgtt
     1921 cgagcacgcg gcgatacacg tttttcagcg gctcgatgtg tgcgacttcg atatgttcgt
     1981 cgatcttgtg gatgctggcg ttcagtggtc cgaattcgat cacctgttcg cagatgcggg
     2041 cgatgaaacg gccatccgag gtgccaccgg tggtcgacag ttcggtcgtt acgccggttt
     2101 cgtccttgat cgctttagcc agcgcgttcg acaggtcgcc gcgcggcgtg aggaacggca
     2161 ggccgctcac ggtccattgc aggtcgtatt cgagctggtg cttgtcgagg atcgcatgca
     2221 cgcgcgcctg caagccttcc accgtgctcg ccgtcgagaa gcggaagttg aacatcacgt
     2281 ccgcatggcc cgcaatcacg ttggtcgcgc cggtgccggc atggaggttc gacacttgcc
     2341 aggtggtggg cgggaagtac tcattgccgt catcccagcg ttcggcgacc agttcggcca
     2401 acgcgggcgc gagcaggtgc accgggtttt tcgccagatg cggataggcg atatggcctt
     2461 gcacgccctt gacgatcagc ttgcccgaca tcgagccgcg ccggccattt ttcaccatgt
     2521 cgccgaattg cgcgctcgag gtcggctcgc cgacgatgca gtagtccatg cgctcgccgc
     2581 gtgcctgcaa cgcttccacg accttgacgg tgccgtcggt agcggggcct tcttcgtcgc
     2641 tcgtaatcag gaaggcgatc gagccgcgat gcgcgggatg cgctgtgacg aattcctcgc
     2701 ttgccaccac gaagccggca atcgacgtct tcatgtccgc cgcgccgcgg ccatagagtt
     2761 tgccgtcgcg atgggtcggc tcgaacggcg ccgaatgcca ttgttcgagc gggccggtcg
     2821 gcacgacgtc cgtgtggccg gcgaacgcga gcagcttgcc cgcggtgccg tcgacgccgc
     2881 gtttgacggc ccacaggttg gtcacgccgt gcgattcgat ggtctcgtgc tcgaagccga
     2941 gagcggacag gcgttcgatc agcaggcgct ggcaatgctg gtcgttgggc gtcacggagg
     3001 cgcgcgcgat cagttgttcg gtaagggcga gggtgccgga catggattca gtacaagcca
     3061 ctttgaaatg aaaaaatgcc ggctggcggt gggacaccgc agccggcaac agcttttgag
     3121 caacgcggcg aggccgcgtt ttacttcttg tggcggtcgg tctttgcgcc aatgcgggct
     3181 tttgatcgtg ccgtccgaat cattgcggcc ggtctcgcgc atcagatcaa gcgcatcaga
     3241 tcacgcaaac agcgccgtat attcgtccac ggaaaaaccg agcgacttga ccttgccgtt
     3301 gaccaccagc accggccgct tgatcaccga aggcttatgg atcatcaacg cgatcgcgcc
     3361 cgactgcgta tccgcggccg ctttcatgtc gtcggacagg ccgcgccacg tggtgccgcg
     3421 acggttcagc aatgcgtcga gcttcacgtc tttcagccag tcctgcacca gcggctcggt
     3481 cacaccgtgc ttcttgaagt cgtggaactc gaactcgacg ccgtgttctt ccagccacac
     3541 acgggccttc ttcacggtgt cgcagttcgg aatgccgtag accacggtct tggtgccgcg
     3601 tgccatcagt cgcctcgcag cagctcgttc aggccgacct tcgcacgggt cttggcgtcg
     3661 accttcttga cgatgacggc gcagtagagg ctgtgcgagc catctttcga cggcaggttg
     3721 cccgccacca ccactgagcc cgccggaatg cgcccgtacg acacttcgcc ggtttcgcgg
     3781 tcgtaaatct tggtgctctg gccgaggtac acgcccatcg agatcacgga gttttcttcg
     3841 acgatcacgc cttccaccac ttccgaacgc gcgccgatga agcagttgtc ttcgatgatg
     3901 accgggttgg cctgcagcgg ctccagcaca ccgccgatgc ccacgccgcc cgacaggtgc
     3961 acgttcttgc cgatctgcgc gcatgaaccg acggtggccc acgtatcgac catcgtgcct
     4021 tcgtcgacgt aagcgccgat gttggtgtac gacggcatca gcacgacgtt cttcgcgatg
     4081 aacgagccgc ggcgcgcgat agcgggcggc accacgcgga agccgccggc ggcgaagtct
     4141 tctgcggtgt agttggcgaa cttcgacggc accttgtcgt agaactggga gtagccaccc
     4201 gccggcatcg gcgcgttgtc ttccaggcgg aacgacagca gcacggcttt cttcagccat
     4261 tgattgacga tccagtcgcc atccttcttt tcggccacgc gcagcgtgcc cttgtccagt
     4321 tgttcgatgg cgtgggcgac ggcttcgcgc acctcggccg gcgcggcctt cggcgacagg
     4381 tcggcgcggt tttcccaggc ggtatcaatg atctgctgaa gttgttgcga catatgcgtg
     4441 cttttgtgaa gagttgaagt ctgaagaagg gaatgcggtg cgcggggttg ctgaatatga
     4501 aaattcgcga aaaacttagc ccgcgagcgc gcggcaaaaa tcgacgattc gttgtgcgcc
     4561 ttgcgtgcat tcgtcgacgt cggcgacgag cgccatgcgc acgaaattgc ggccggggtt
     4621 cacgccgtgc gcagtacgcg cgagaaacga gcccggcaaa accgtcacat tatagtcggc
     4681 gtagaggcgc tgggcgaact cggtgtccgt caggccggtg cgcgagacgt tcgcccacag
     4741 gtagaacgcg gcgtccggca ggcgtacgtc gagcacctcg gcgagcatcg gcgtgacggt
     4801 ggcaaacttc tgcacgtact tcgcgcggtt ctcgcgcacg tgcgtttcgt cattccaggc
     4861 cgcgatgctg gcattctgaa acaccgtcga caaggccgcg ccgtggtatg tccggtatag
     4921 caggaagtct ttcaggatcg ccgcgtcgcc cgccacgaag cccgagcgca tgcccggcac
     4981 gttcgatcgc ttggacaggc tcgagagcat cacgagccgc tcgaagccac ggccgagctt
     5041 gtgcgcggct tccaggccac ccagcggcgg attggtctcg tcgaagtaga tttccgagta
     5101 gcactcgtcc gaggcgatca cgaagccgta acggtccgac agtgcgaaca gttcgcgcca
     5161 gtcgtcgagc gtgagcacgg cgccggtagg attgcccggc gagcacacgt acagcagctg
     5221 cgtgcgcgcc cagatgtccg ccggaaccgc cgcatagtcg caggcgaagt tgcgcgccgg
     5281 atcgctattc acgaagtatg gctgcgcgcc ggcaaggatc gtcgcgcctt cgtagatctg
     5341 gtagaactgg ttcgggcaga gtacgatcgc aggctcgccg tcggcatggc gcgcgttgtt
     5401 ccgcgcagtg tcctgtttag ggtcgatgac cgtttgtgcg agcgcaaaaa gcgcctcgcg
     5461 cgagcccgac accggcagca cctgggtggc cggatcgacc ggcggcaggt tgtagcgctg
     5521 cgtcacccag gtcgcgatcg actcgcgcag tgccggcgaa ccgatggtgg ccggataggc
     5581 cgacaatccg ccgagcgagg cgatcaccgc gtcgcgaatc agcgcgggcg tgggatgttt
     5641 cggttcgccg atgccgaagc tgatgtgcgc gaggccggcc ggcggcgtga cgtccttgaa
     5701 aagcgcacgc agcttttcga acggataggg atgaagggag tcgagtagcg ggttcactgg
     5761 gcggatcgag cctgatcgaa gagggcgcag acggatgcag ggaaatgccg ggcacgcgcg
     5821 ctgacggcaa aacacctgaa agacgcgaaa acggtggcag cggcggcaag cagatgatta
     5881 tagcgcgagc ccactgcagc ggcgccaggc gtgtaaaacg cggcgcaacc cgccaggcag
     5941 cgcaagcaat gcgacgcagc gggaacgacg cgaaccgcgg acggttcgcg caaaaaagaa
     6001 gcaggagcag caatgtacgc aacagcaacg gagacatcca cacgatttgg gcaacgcggg
     6061 ccgcgcgggc agcagggggc actcggacaa ttcgaacaag tcggactacc gggacgaaga
     6121 ggacgaagca gccacaagaa cttcgccatt gccaaagtcg gcaacgccgc ccgcacgagc
     6181 agtcatcggc gcgggagcct cgcatcatga cccactggct tcgcaatggc tggcctaccc
     6241 tggcgatcat gctgggcgcc tcggtgtggg gggtgatctg gtacccgctg cgcatgctga
     6301 atgcgctcgg cgtcaccggc acggcggcga gcgcgctgac gagcggcgcg ggttgcctgt
     6361 tcgtcctgct ggtgcgccat cgggcgctca agaccgtgcg ctggcactgg ctgctgccgg
     6421 ctctggcgct ggccgcgggc atcaccaatc tcggcttcgt gtggggttcg atccacggcg
     6481 aagtgatgcg cgtgctcctg ctgttctatc tcacccccgc ctggaccgcg ctcttcgcgc
     6541 acttcatcct gcacgaacgg ctgacgtggg cgggcgcggg cctcgccgcg ctctcgctgg
     6601 ccggtgcgat gaccatgttg tggtcgccgc aactgggcct gccggtgccc ggcaacctcg
     6661 ccgaatgggc cggacttgcg ggcggcatgg gcttcgcgat gagcaacgtg ctgatcctca
     6721 agacgagccg cgtgctgcct gagatgaaac cggaaatgcg caccgcgacg atcttcggcg
     6781 gcgcggcggt gttcagcgct ttggcgtcgc tgttcgaggc gatgccggca ccacccaccg
     6841 gcgcgcatct caacacggcc gcgctactgg tgcttggcct cggttttctg ctggcgtcga
     6901 acaatatgct cgtgcagtat ggactctcgc gcgtgccggc caatcgggcc tcgatcatca
     6961 tgctgttcga gatcgtggtc acggccttgt ccgcgtggct cttcgccggc gagacgccgg
     7021 gcccgcgcga atgggccggc ggcgcgtgca tcgtgctggc gtcggtgcta tccagttggg
     7081 tgcaccggac ccgtgcgttg ccgcctgatc cggccaatca ggatgcgggt ctgaacgccg
     7141 ccgaaggcac ggaccgtaag gacggtaaga accgctcacg cgcgatggta tgattgcccc
     7201 tcattagccg gcgcacgctt gattgcgttc gcctggcacc cgtatcgcgg ggtttcgcgg
     7261 gccgtttcgc tgcatcggcg aacggctggc gcggccttca cgagccacct tttattttcc
     7321 cttttcaaac agcgaaatcg ccgtgcgtct gacctcgatc aaactcgctg gcttcaagtc
     7381 attcgtcgat cccacgcatt tccaggtacc gggccagctc gtcggcgtgg tcgggccgaa
     7441 tgggtgcggc aagtccaaca tcatcgacgc cgtgcgctgg gtgctcggcg aatcgcgcgc
     7501 ttccgagctg cgcggcgagt cgatgcagga cgtgatcttc aatggctcga ccgcgcgcaa
     7561 gccaggtagc cgcgccagcg tcgaactcgt gttcgataac gccgacggcc gtgccgccgg
     7621 gcagtggggg cagtatgccg aaatcgccgt gaagcgcgtg ctcacgcgcg acggcacctc
     7681 gagctactac atcaacaacc tgccggcgcg ccgccgcgac atccaggaca tcttcctcgg
     7741 cactggcctc gggccgcgtg cgtacgcgat catcgggcag ggcatgatcg cgcgcctgat
     7801 cgaggcgaag ccggaagagc tgcgcgtgtt cctcgaagaa gccgcgggcg tgtcgaaata
     7861 caaggaacgc cgccgcgaaa ccgagaaccg tctgcacgac acccgcgaga atctgatccg
     7921 cgtcgaagac atcgttcgcg aactcagtgc gaatctcgag aagctcgagg cgcaggcgat
     7981 cgttgcgacc agatataagg atttgcaggc cgacggcgaa gagaagcagc gcctgttgtg
     8041 gctgttgcgc aagaacgaag ccggcggtga acaggagcgc cagcaacgcg cgatcgaaca
     8101 ggcacagatc gacctcgaag cgcaaaccgc gaagctgcgc gaagtcgagg cgcaactcga
     8161 aacactgcgg gtcgcgcatt attccgctag cgacgcgatg cagggcgcgc aaggcgcgtt
     8221 gtacgaggcg aattccgaag tcagccggct tgaagccgaa atcaggttca tcgtcgaatc
     8281 gcgcaaccgc gtgcaggcgc agatcgctgc attgaccgcg cagcgtgaac agtggcagtc
     8341 gcaagcccaa aaagcgcaag atgatctcga agacgctgaa gaacaactcg ccgtcgccga
     8401 agaaaaagcc gcgctggccg aagacgaagc cgccgccaaa catgatgcga tgccggcgct
     8461 ggaagcgcgc tggcgcgacg cgcagaccga actgaacgcc gaacgtggcg gtatcgcgca
     8521 gaccgaacag gcgttgaagc tcgaagccgc gcatcagcgc aatgccgatc agcaattgca
     8581 gcagttgcag cagcgtcacg agcggctgaa gtcggaagcg ggcggattgg acgctcccga
     8641 cgaagcgcaa ctcgaagagc tgcgcatgca gctcgccgag cacgaagaga tcctgcatga
     8701 cgcgcaaacg cgcctcgccg acgcgcagga aaccctgccg cgtctggacg gcgaacgccg
     8761 tagcgcgcag gagcgcgtgc agtcggaaag cgcccagatt catcagctcg acgcacgcct
     8821 ggccgcgctc aagcagttgc aggaaaacgt ccagaccgaa ggcaagatcc agccgtggct
     8881 tgaaaagcac gagttgaacg gcctgccgcg tctgtggaag aagctgcatg tcgaagccgg
     8941 ttgggaagcc gcgctcgaag cggtgctgcg cgagcgcctt gcggccctgg aagtgtcgaa
     9001 cctcgactgg gtcaaggctt tcgctaccga cgcgccgccc gccaagctcg cgttctacgc
     9061 accgccggca gccggtcagc cggtcgttac gccgccggcc ttgcggccgc tgctgtcgct
     9121 ggttcgcatc gacgatgcgg gtattcgcgc cgtgctgaac gactggctcg gcctcgcctt
     9181 tgtcgctgac gatctgcaac aggcgctcgg catgcgcgcg caattgccgg aaggcggctc
     9241 gttcgtcgtg aaggccggcc acgtggtgac gcgtgtcggc gtccagctgt atgccgccga
     9301 ctccgaacag gccggcatgc tggcccgtca gcaggaaatc gaaaacctga cgcgccaggt
     9361 gcgtgcgcaa gccttgctcg ccgatgaggc aaaggccgcc gcgatccgcg ccgaagccgc
     9421 gcacacgcaa gctgcgcaag cattgaccga tgttcgtcaa cagtcagagc gcgcgacgca
     9481 acgcgtgcat gcattgcaga tggacgtgct caagctcgcg caagcgcatg agcgttacac
     9541 gcagcgcagc acgcaaatcc gcgaagagct cgcggaaatc accgcgcaga tcgaagagca
     9601 acgcgctttg cgtggcgaat cggaagcgaa cttcgagcgt cacgattccg aactcgccga
     9661 attgcaggcg cgtttcgaag atcaccagat ggccttcgaa gcactcgacg aagcgctcac
     9721 ctccgcacgc acccaatcgc gcgatctcga tcgggccgcc accgacgcgc gcttcgccgc
     9781 gcgcaacatg gcgaaccgca tcgacgaatt gaagcgcagc atccaggtct cgcacgagca
     9841 gagcgagcgc gttgccggct cgctcgaaga cgcgcgcgcc gaactggaaa cgatcaacga
     9901 gcaaaccgcg cataccggct tgcaggacgc gctcgatatc cgcgcggtca aggaagaagc
     9961 gctgcatgcc acgcgtctcg aactggacga cctgaccgcc aagctccgcg ccgccgacga
    10021 aacccgtttg accgccgagc gtgcgcagca gccgctgcgc gagcgcatca acgaattgca
    10081 gttgaaggaa caggccgcgc gcctgaacgg cgagcagttc atcgagcaac tggccgcggc
    10141 cggcgtcgat gaggcggagt tgcaggccaa gctcacgccg gacatgaagc cgtcctacct
    10201 gcaaggcgaa gtcacgcgga tcaacaacgc gatcaccgcg ctcggcccgg tcaatatggc
    10261 cgcgctcgac gaactgaagg cggcgaccga gcgcaagacg ttcctcgact cgcaatcggg
    10321 cgacctgaac aacgcgatcg aaacgctcga agacgcgatc cgcaagatcg acggcgaaac
    10381 gcgtacgctg ctgcaaggca ccttcgacga agtgaaccgt catttcggcg aactgttccc
    10441 gcgtctcttc ggcggtggcc aggccaggct gatcatgacc ggcgacgaaa ttctcgacgc
    10501 cggcgtgcag gtcatggcgc agccgccggg caagaaaaat tcgacgattc acctgctgtc
    10561 gggcggcgag aaggccttga ccgctaccgc gctggtgttc gcgatgttcc agctgaatcc
    10621 cgcgccgttc tgtctgctcg acgaagtgga cgcgccgctc gacgacgcca acacggaacg
    10681 cttcgcgaac ctcgtgcgcg cgatgtcgga caagacgcaa ttcctgttca tttcgcacaa
    10741 caagatcgcg atggaaatgg cgcagcaact gatcggcgtg accatgcagg agcagggtgt
    10801 atcgcggatc gtggccgtcg acatggaaac ggctgcgggt ttcgcccaga atatcggtta
    10861 ggttttcgtt taggttgatc atcgcgggcg cccgcgttca gcgcagcggc catcggccgc
    10921 gcgacacgtg gggcgggcgc agagccgcgc agacagaaga attgctgatg gagcatgcat
    10981 ggacgagttg acactcggtt tgatcggcgc gggtgccgta gtggtcgggg gcgtggtcgt
    11041 gtacaacgca tggcagggcg cgaaggtgcg ccgcaagatg ccgcgtccca tgccggccga
    11101 caccgccgaa agcttcgcgc ggga
//
LOCUS       DATASH010001121         8555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5537732,
            whole genome shotgun sequence.
ACCESSION   DATASH010001121 DATASH010000000
VERSION     DATASH010001121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 8555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8555
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5537732"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="VHV99_29770"
     CDS             <1..636
                     /locus_tag="VHV99_29770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014896025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HEX3639598.1"
                     /translation="MRWELHDASVKPRVIIMVSKIGHCLNDLLFRYRTGQLAIEIPAI
                     ISNHKEFYQLAASYDIPFHHFPLLGGTPDAKAAQEARVLEVIDEHQADLVVLARYMQI
                     LSPKLCEALAGRAINIHHSFLPSFKGAKPYYQAFDRGVKLIGATAHYVTTDLDEGPII
                     EQEVERVDHSMTPEQLTAIGRDVECVTLARAVKWHVEHRVVLNGSKTVVFR"
     gene            complement(856..2091)
                     /locus_tag="VHV99_29775"
     CDS             complement(856..2091)
                     /locus_tag="VHV99_29775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HEX3639599.1"
                     /translation="MRQQLVRLHRWSGVAIALFLFLAGLTGAIIAWDHELDAALNPSF
                     FKARTAGPALSGLELARRVEAADPRLQVTYLPLHTEPGHTLQMMVLPHTNPATKQPYS
                     LDFNQIAVDPATGDVQGRREWGAVSLARLNLIPFIYKLHYTLQLPFTGGVDIGTWLMG
                     IVGIVWLLDSVVALWLSFPSFKAWRKSFAFRLTRGGYALTFDLHRSGGVWIWGLLVIV
                     ALTSVSMNLSAPVVRPIVSMFSTLTPDPINNPEILRKPEPGDQVLSRERIVQLAEQAG
                     KAQNLKEAPGGLYYAEFLHAYGVGFYATGNDHGDVGLGNPWMYWDAATGKQLGAQIPG
                     KGTAGDIFMQVQFPLHSGRILGLGGRILISAVGIAVAVLSATGLLIWLKKLDARRRSA
                     QNARLAQNARDAGRTAVRS"
     gene            2210..2593
                     /locus_tag="VHV99_29780"
                     /pseudo
     CDS             2210..2593
                     /locus_tag="VHV99_29780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013094227.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(2652..3737)
                     /locus_tag="VHV99_29785"
     CDS             complement(2652..3737)
                     /locus_tag="VHV99_29785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX3639600.1"
                     /translation="MAKRNQARDEGQVQDLRPRPVRLTSDMSLPKLSAVEIGSYVLML
                     FGMWAVIELRLLGALLAGLLVFQLVHTIAPRIERHMSSQRARWLAVVILSALIVGALT
                     GLTLGIIEHFENDVPSVQKLLDQAMQLIDQARGRIPQFVANYLPVDTEQMKTKAAELM
                     QTHANMLQQSGKSVARAFTHILIGMIIGAIIAVGAQKHMQRLPLSTAFVTRVTRFADA
                     FRRIVFAQVKISAINAVFTGIFLLVILPIFHDTLPLSKTLVLVTFIVGLLPVIGNLIS
                     NTIIVAVALSVTFPAAIMSLVFLILIHKLEYFLNARIVGGQIEARAWELLVAMLVMEA
                     AFGIPGVVAAPIFYAYIKRELIYLRLV"
     gene            complement(3772..6645)
                     /gene="uvrA"
                     /locus_tag="VHV99_29790"
     CDS             complement(3772..6645)
                     /gene="uvrA"
                     /locus_tag="VHV99_29790"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX3639601.1"
                     /translation="MEQIRIRGARTHNLKNVNLDLPRHKLVVITGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLQLMEKPDVDLIEGLSPAISIEQKATSHNPRSTVGTVT
                     EIHDYLRLLFARVGTPYCPDHEIPLEAQSVSQMVDAALALPEETKLMILAPVVANRKG
                     EHVELFEEMQAQGFIRFRVRSGGGTANEGVAKIYEVDSLPKLKKNDKHTIDVVVDRLK
                     VRGDMKQRLAESFETALRLADGRAIALEMDTDKEHLFSSKFACPICSYSLQELEPRLF
                     SFNNPMGACPECDGLGQITFFDPKRVVAHPSLSLAAGAVKGWDRRNQFYFQMLQSLAA
                     FYEFDIDTAVEDLPEKVRKILLFGSGKEVIPFSYINERGRTSVREHVFEGIIPNLERR
                     YRETDSVAVREELAKYQNNQPCPACAGTRLRREARFVRIGSDGDARGIFEISGWPLRD
                     ALGYFQTLRLEGSKREIADKVVKEIVARLMFLNNVGLDYLSLERSAETLSGGEAQRIR
                     LASQIGSGLTGVMYVLDEPSIGLHQRDNDRLIATLKHLRDLGNSVIVVEHDEDMIRMA
                     DYVVDMGPGAGEHGGMVIAEGTPKQVQANPASMTGQYMSGTRNIEFPDERKEPDERRL
                     RIVEAFGNNLQHVSLDLPVGLLTCVTGVSGSGKSTLINDTLYHAVAHHLYGSSTEPAP
                     YESIEGLEHFDKVINVDQSPIGRTPRSNPATYTGLFTPIRELFAGVPAAKERGYEAGR
                     FSFNVKGGRCESCQGDGVLKVEMHFLPDVYVPCDVCHGKRYNRETLDVQYKGKNISEV
                     LDMTVENAYEFFKPVPVVARKLKTLLDVGLGYIRLGQSATTLSGGEAQRVKLSLELSK
                     RDTGRTLYILDEPTTGLHFHDIALLLEVIHRLRDQGNTVVIIEHNLDVIKTADWVIDL
                     GPEGGAGGGQIIAQGTPEQVARSKASFTGKYLAPLLKRTASRK"
     gene            7000..8193
                     /locus_tag="VHV99_29795"
     CDS             7000..8193
                     /locus_tag="VHV99_29795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011886043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3639602.1"
                     /translation="MSNPSATPSRMSAPEMRATMSLAAIFALRMLGLFMIMPVFSIYA
                     KTIPGGDNVLLVGIALGAYGVTQSMLYIFYGWVSDKVGRKPVIATGLLIFALGSFVAA
                     GAHDMTWIIIGRVIQGMGAVSSAVIAFIADLTSEEHRTKAMAMVGGSIGISFAVAIVG
                     APIVFQWVGMSGLFTLVGIFSILAVGLVVWVVPDAPKPVHVRAPFAEVLHNVELLRLN
                     FGVLVLHATQTALFLVVPRILEAGGLPVASHWKVYLPVMGLSFVMMVPAIIAAEKRGK
                     MKIVLLSAIGLILIGQLLLGVAPHTILSVAAILFVYFLGFNILEASQPSLVSKLAPGT
                     RKGAAAGVYNTTQSIGLALGGVIGGWLLKVDGQSAVFFTCSGLVFCWLIIAANMKQPP
                     PRKKA"
     gene            8433..>8555
                     /gene="ssb"
                     /locus_tag="VHV99_29800"
     CDS             8433..>8555
                     /gene="ssb"
                     /locus_tag="VHV99_29800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX3639603.1"
                     /translation="MASVNKVILVGNLGADPEVRYLPSGDAVANIRLATTDRYKD"
BASE COUNT         1596 a         2617 c         2675 g         1667 t
ORIGIN      
        1 atgcgctggg aattgcatga tgcgtccgtg aagccgcgcg tcatcatcat ggtgtcgaag
       61 atcggccatt gcctgaacga cctgctgttc cgctatcgca ccggtcaact ggcgattgag
      121 attccggcga tcatctcgaa tcacaaagag ttctaccagc tcgccgccag ctacgacatt
      181 ccgttccatc acttcccgct gctgggcggc acgcccgacg cgaaggccgc gcaggaagcg
      241 cgcgtgctcg aagtgatcga cgaacatcag gcggatctgg tggtgctcgc gcgctacatg
      301 cagattctgt cgccgaagct gtgcgaagcc ctggccggcc gcgcgatcaa cattcatcac
      361 tcgttcctgc cgagcttcaa gggcgcgaag ccgtattacc aggcgttcga ccgcggtgtg
      421 aagctgatcg gcgcaaccgc tcattacgtg acgacggatc tcgacgaagg tccgatcatc
      481 gagcaggaag tggagcgcgt cgatcacagc atgaccccgg agcaactgac ggcgatcggc
      541 cgtgacgtcg aatgcgtgac gctcgcgcgc gcggtgaagt ggcacgtcga gcatcgcgtc
      601 gtgttgaacg gtagcaagac ggtggtgttt cgttaagaga agttgatgcg ctggcggtgg
      661 ggacggttgc cggaccatgg ccgcatggcc ccgctgcaag cgtgtaccga atcagtcagc
      721 cagcgcggtc tctctccatg gccgtgcaag ctgctgcaag tgcgttgcga atgaatgcgg
      781 cgcgctcgtt tcagatcggg gccgcgcaag ttgttgcaag tgcgtttcga atgaatgcgg
      841 cgctctcgtc tcagatcagg accgcaccgc ggttctgccc gcatcccgcg cgttctgtgc
      901 gagccgtgcg ttttgcgccg aacggcggcg tgcatccagt ttcttcagcc agatcagcaa
      961 tcccgttgcg ctcagcacgg cgaccgcaat ccccaccgcg ctaatcagaa tccgtccgcc
     1021 gagaccgagg atgcgtcccg agtgcagcgg gaattgcacc tgcatgaaga tatcgcccgc
     1081 cgtgcccttg ccggggatct gcgcgccgag ctgcttgccg gtggcggcgt cccaatacat
     1141 ccaggggttg ccgagcccca catcgccgtg atcgttgccg gttgcgtaga agccgactcc
     1201 gtaggcgtgc aggaattccg cgtagtagag gccgcccggt gcctccttga ggttttgcgc
     1261 tttgccggct tgctcggcca gttgcacgat gcgctcgcgg ctcaatacct gatcgcctgg
     1321 ctcaggcttg cgcagaatct cggggttgtt gatgggatca ggcgtgagcg tcgagaacat
     1381 cgacacgatt ggccgaacga ccggcgccga aagattcatc gacaccgacg tcagcgcgac
     1441 gatcaccagc aagccccaga tccacacgcc gcccgagcgg tgcagatcga aggtgagcgc
     1501 atagccgccg cgcgtgaggc gaaacgcgaa cgacttgcgc catgccttga agctcggaaa
     1561 cgacagccat agcgcgacga cgctatcgag cagccacacg atcccgacaa tacccatcag
     1621 ccacgtgccg atatcgacac cgccggtaaa cggcaattgc agcgtgtagt gcagcttgta
     1681 gatgaacgga atcagattga gccgcgcgag cgaaacggcg ccccattcgc gcctcccctg
     1741 aacgtcgcca gtggcagggt cgacggcaat ctgattgaag tcgagtgagt agggctgttt
     1801 cgtggcgggg ttggtgtgcg gcagcaccat catctgcagt gtgtggccgg gttcggtgtg
     1861 gagcggcaga tacgtcacct gcaggcgcgg atcagccgct tcgacgcgac gggccaactc
     1921 gagccccgac agcgccgggc cggcggtgcg cgctttgaag aacgacgggt tcagcgccgc
     1981 atcgagttca tgatcccacg cgatgatcgc gccggtcagg ccggcgagga acagaaacag
     2041 cgcaatggcg acaccggacc agcggtgcag ccggacgagc tgctgcctca taagcgagcc
     2101 ggcgagacgc ggcgggaatt ctgacggggt gtcacgacaa agacataagt gcaaatggga
     2161 atcgttcgca tcttacggaa tcctgccagc agccgcaagt ttcttgtaag tcggcggcga
     2221 cctcatgtgg atcaacacga agtacgagaa gggcgcggct aacaacggca atcgcgtggc
     2281 cggcgcgccg caattcgtcg tggcaggcca tgcggcgtac gcggtgcttt atgtgccggg
     2341 gctgcgcatc ggtgccgacg cgaagttcac cggcaatacc aacgtgcgcc ccaccgccaa
     2401 tcttaatgcg ccgggctaca tgctggtcaa ccttggtgcg agctacgcca cgcgcatcgg
     2461 cggctacgac gtgacgttcc gcgcggcgat cgacaacctg ctcaatcggc gttactggga
     2521 gtatcagtac gcggactccg tgacgccagg cgatccgcgt acgttgtcgc tgaacgcgcg
     2581 catcgacttc tgagcatgaa aaaagcggca gtgacatgcc gcttcagact gccgatcaac
     2641 ccggccctcg ctcacaccag gcgcagatag atcaattccc gcttgatgta cgcatagaag
     2701 atcggcgccg cgaccacgcc ggggatgccg aacgcggctt ccatcacgag catcgcgacc
     2761 agcagttccc aggcgcgtgc ctcgatctga ccaccgacga ttcgcgcatt caggaagtac
     2821 tccagcttgt gaatcaggat caggaaaacg agcgacatga tcgccgccgg aaaggtcacc
     2881 gaaagcgcca ccgcgacaat gatcgtgttc gagatcagat tgccgatcac aggcagcaag
     2941 ccgacgatga acgtcaccag cacaagcgtt ttggagagtg gcagcgtgtc gtgaaagatc
     3001 ggcaggatga ccagcaggaa gatgccggtg aagaccgcgt tgatcgcgga aatcttgacc
     3061 tgagcgaata cgatgcggcg aaaggcgtcg gcaaaacggg tcacgcgcgt gacgaatgcc
     3121 gtggacaaag gcagccgctg catatgcttc tgcgccccga cggcgatgat cgcgccgatg
     3181 atcatgccga tcaggatgtg cgtgaaagcg cgcgcaacag acttgccgct ttgctgcagc
     3241 atgttcgcgt gcgtctgcat cagctcggcc gccttcgtct tcatctgctc ggtatcgacc
     3301 ggcagatagt tggcgacgaa ttgcggaatc cggccgcgcg cctggtcgat caactgcatc
     3361 gcctggtcga gcagcttctg gacgctcggc acgtcgtttt cgaaatgttc gatgatgccg
     3421 agcgtcagcc ccgtcagcgc accgacaatc agcgccgaga ggatcaccac cgcgagccag
     3481 cgcgcgcgct gactcgacat atgccgttcg atgcgcggcg caatggtgtg cacgagttga
     3541 aacacgagca gcccggccag caaggcgccg agcaacctca gttcgatgac cgcccacatg
     3601 ccgaacagca tcagcacgta gctgccgatt tcgaccgccg acagcttcgg caggctcatg
     3661 tcgctagtca gcctgaccgg gcgtgggcgt aggtcctgca cttgcccttc gtcgcgcgcc
     3721 tgattccgct tggccatggc ttatcccgtt tccaacccgt gttgtgcagc gttactttct
     3781 gctggcggtg cgtttcagca gcggcgccag atattttccg gtaaaacttg cctttgacct
     3841 ggcgacctgc tccggcgtgc cttgcgcgat gatctgaccg ccgccggcac cgccttccgg
     3901 accaagatcg atcacccagt cggcggtctt tattacatcg agattatgct cgatgatcac
     3961 gacggtatta ccctggtctc gcaaacgatg aatgacttcc agcagcaagg cgatgtcgtg
     4021 aaagtgcaga ccggtggtcg gctcgtcgag gatgtataga gtgcgaccgg tgtcgcgctt
     4081 gctcagttcc aaagatagtt tgacgcgctg cgcctcgccg ccggagaggg tggtggccga
     4141 ctggcccagc cggatatagc ccagacccac gtccagcaag gttttcagct tgcgcgcgac
     4201 aaccggcaca ggcttgaaga actcgtaggc gttctccacc gtcatatcga gcacttcgct
     4261 gatgttcttg cctttgtact gcacgtcgag cgtttcgcgg ttgtagcgct tgccgtggca
     4321 gacatcgcag ggaacgtaga cgtccggcaa aaagtgcatc tccaccttca gcacgccgtc
     4381 gccctggcag gattcgcagc ggcctccctt cacattgaac gagaagcggc cggcttcgta
     4441 gccgcgttcc ttcgcggcgg gcacgcccgc gaacaactcg cggatcggcg tgaacagtcc
     4501 ggtgtaagtg gccggattcg agcgcggcgt gcggccgatc ggtgactggt ctacgttgat
     4561 gaccttgtcg aagtgctcca gaccttcgat cgactcgtac ggcgccggct cggtggatga
     4621 tccatacagg tgatgcgcga ccgcgtgata cagcgtgtcg ttgatcagcg tggacttgcc
     4681 cgaaccggac acgccggtca cgcaggtcag caggccgacc ggcagatcga gcgagacgtg
     4741 ctgcagattg ttgccgaacg cctcgacgat ccgcagacgc cgctcgtccg gctccttgcg
     4801 ctcgtccggg aattcgatgt tgcgggtgcc ggacatgtac tgcccggtca tcgacgcagg
     4861 attcgcctgc acctgtttgg gtgtgccctc ggctatcacc atgccgccgt gctcacccgc
     4921 tccaggtccc atgtcgacca cgtagtcggc catgcggatc atgtcctcgt catgttcgac
     4981 gacgatcacc gagttgccca ggtcgcgcag atgcttgagc gtggcgatca gccggtcgtt
     5041 gtcgcgctgg tgcagaccga tcgacggctc gtccagcacg tacatcacgc cggtcaaacc
     5101 ggacccgatc tgcgaggcga ggcgaatccg ctgcgcttca ccgcccgaga gcgtttccgc
     5161 gctgcgttcg agcgacagat agtcgagtcc gacgttattc aggaacatca gccgcgcgac
     5221 gatttccttg accaccttgt cggcgatctc gcgcttggag ccttcgagcc gcagcgtctg
     5281 gaaatagcca agcgcgtcgc gcaacggcca gccgctgatt tcgaagatgc cgcgcgcgtc
     5341 gccgtccgag ccgatccgca cgaaacgggc ttcccgacgc agccgcgtgc cggcgcaagc
     5401 ggggcagggc tggttgttct gatacttggc gagttcttcg cgcaccgcga ccgaatcggt
     5461 ctcgcggtaa cgccgctcca ggttcgggat gattccttcg aacacatgct cgcgcacgga
     5521 agtgcggccg cgctcgttga tgtacgagaa cggaatgacc tctttgccgg agccgaacag
     5581 caggatcttg cggactttct ccggcaagtc ttcgactgcc gtgtcgatgt cgaactcgta
     5641 gaacgccgcg agactttgca gcatctggaa gtagaactgg ttgcgccggt cccagccctt
     5701 caccgcgcct gccgccagag acagcgacgg atgcgccaca actcgcttcg gatcgaagaa
     5761 ggtgatttgc cccaggccgt cgcattccgg gcacgcgccc atcgggttgt tgaacgagaa
     5821 gaggcgcggc tccaactcct gcaacgaata cgagcagatc gggcaggcga acttcgagct
     5881 gaacagatgc tccttgtccg tatccatttc cagcgcgatg gcgcggccgt cggcgagacg
     5941 cagtgccgtt tcgaacgatt cggccagacg ctgcttcata tcgccgcgga ccttcagacg
     6001 atccacgacc acgtcgatcg tgtgtttgtc gttctttttc agcttcggca gtgaatcgac
     6061 ttcatagatc ttcgccacgc cttcgttggc ggtgccgccg cccgagcgca cgcggaagcg
     6121 gatgaagccc tgcgcctgca tctcctcgaa cagctcgacg tgctcgccct tgcggttcgc
     6181 caccactggc gcgaggatca tcagtttcgt ttcttccggc aacgcgagcg ccgcgtcgac
     6241 catctgcgag acgctttgag cttccagcgg aatttcgtgg tcagggcagt acggcgtgcc
     6301 gacgcgcgcg aacagcagcc gcagatagtc gtgaatttcg gtgacggtgc cgaccgtgga
     6361 acgcggattg tgcgaggtcg ctttctgttc gatcgagatc gccggcgaca ggccttcgat
     6421 cagatcgacg tccggctttt ccattaattg caggaattgg cgggcataag cggaaagact
     6481 ttcgacgtag cgacgctgtc cttccgcata gagcgtgtcg aacgccagcg acgatttacc
     6541 cgagccggac aggcccgtaa tcaccacgag cttgtgacgt gggaggtcga gattgacgtt
     6601 cttcaggttg tgggtgcgag ccccacggat acggatttgt tccacgcgtt attccatcac
     6661 ctggccatga gctggcggaa agaggaagag gctaaacctg ctactataac gacttttcaa
     6721 gaccgccgtt acggcttcct gacagccgaa agaaggcaca aggcaagctg taaggcagcg
     6781 gtccggcgcc gtggggaaga ggtctaactg gggccgtttc ccgcgagtac aaggcgccgc
     6841 gaagttccag gcgagtgtgc agcaagaccg ccgggccgca cgccgcgcca tgtgtttcac
     6901 gctatccagc cgtcccgtgg taaatcgcct ggttttggcc gtcctgcggg gaaggtccgc
     6961 ccgtggtgtg ccgcccagct ctttcaaaga acgctcccga tgtccaatcc gtccgctaca
     7021 ccctcacgca tgagcgcgcc tgaaatgcgc gcgaccatgt cgcttgccgc catcttcgcg
     7081 ctgcgcatgc tcggcctctt catgatcatg ccggtgttct cgatctacgc gaagaccatt
     7141 ccaggcggcg acaacgtgct gctggtgggt atcgcgctcg gtgcgtacgg tgtgacgcag
     7201 tccatgctgt acatcttcta cggctgggtc tccgacaagg tcggccgcaa accggtcatt
     7261 gcgaccggcc tcctgatctt cgcgctcggc agcttcgttg cggcgggcgc gcacgacatg
     7321 acgtggatca tcatcggccg ggtgattcag ggcatgggcg cggtgtcgtc cgcggtgatc
     7381 gcgtttatcg ccgacctgac ctccgaagag catcgcacca aggcgatggc gatggtcggc
     7441 ggcagcatcg gcatttcgtt cgcggtggcg atcgtcggtg cgccgatcgt gttccagtgg
     7501 gttggcatga gcggcttgtt cacgctggtc ggcatcttct cgattctggc ggtcggcctg
     7561 gtggtgtggg tcgtgcctga tgcgcccaag cccgtgcacg tgcgcgcgcc gttcgccgag
     7621 gtgctgcata acgtcgaact gctgcgtctg aacttcggcg tgctcgtgct gcacgctacg
     7681 caaaccgcgt tgttcctcgt ggtgccgcgc attctcgaag ccggcggcct gccggtcgcg
     7741 tcgcactgga aggtgtattt gccggtcatg ggcctttcgt tcgtgatgat ggtcccggcg
     7801 atcatcgctg ctgagaaacg cgggaaaatg aaaatcgtgc tgctgtccgc gatcggtctt
     7861 atcctgatcg gacagttgtt gttgggcgtc gcaccgcata cgattctgag tgtggcggcg
     7921 attctttttg tctactttct cggcttcaat attcttgagg catcgcagcc ttcactggtg
     7981 tcgaaactgg cgcccggcac ccgcaagggt gcggcggccg gcgtgtacaa caccacgcag
     8041 tcgatcggtc ttgcgctggg tggcgtgatc ggcggctggt tgctgaaagt ggatggtcag
     8101 agcgcggtct tcttcacttg ctcggggctg gtgttttgct ggcttataat cgcggcaaat
     8161 atgaaacagc cgccgccgcg caagaaggcg taacagccgc ccccaaccct ggcgctttgc
     8221 gccaagcctc tcgaggggga agtcccccga tcccccaagg ggacttcctt tggggcgggg
     8281 gacggggcgg aaagcctggg acggcccggc gttcttttga tagaactcac ctggcggcgg
     8341 cctgaggcgc aattccggtc tccggcccgc tggcttgaga cgtgaattgt gcattgccgt
     8401 gccgcccgca acgaaatcaa caggagaaac tcatggcatc cgtgaacaag gtcattctcg
     8461 tcggcaatct cggagccgat ccggaagtcc gttatcttcc gagcggcgac gcagtggcga
     8521 acatccgcct tgcgacgacg gatcggtaca aggac
//
LOCUS       DATASH010001122         4252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4149342,
            whole genome shotgun sequence.
ACCESSION   DATASH010001122 DATASH010000000
VERSION     DATASH010001122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4252
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4149342"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VHV99_29805"
     CDS             <1..293
                     /locus_tag="VHV99_29805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011350958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HEX3639604.1"
                     /translation="SQADAERALEDFNHRAKGGVPDDIPAVTLAGAPLAIGQLLKQAN
                     LVPSTSEALRNIEQGGVKIDGTTVSDKGLKVEAGEYVVQVGKRRFARVTLTA"
     gene            346..804
                     /gene="dtd"
                     /locus_tag="VHV99_29810"
     CDS             346..804
                     /gene="dtd"
                     /locus_tag="VHV99_29810"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010091359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEX3639605.1"
                     /translation="MIALIQRVRRAEVRVGDRVTGAIEAGLLALVCAERGDTEGAADR
                     LLAKVLGYRVFSDAAGKMNLSVQNLDGAGRAGGLLLVSQFTLAADTNSGLRPSFTPAA
                     PPDEGKRLFDYFVSAARAKHPIVETGEFGADMQVSLVNDGPVTFWLQTNP"
     gene            916..1596
                     /locus_tag="VHV99_29815"
     CDS             916..1596
                     /locus_tag="VHV99_29815"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX3639606.1"
                     /translation="MTTQILFIRHGETDWNRIKRIQGHIDIPLATTGLAQAQSLASRI
                     ANEVKEGARLDAIYSSDLLRAQQTAQPIADALGLPVQLSEGLRERSYGAFQGHDSDEI
                     AVRFPDEYAHWQTRDPGFAPPEGESQRAFYHRVLHAVEPIVAAHPGRRVAVVAHGGVL
                     DCVRRFAARLSLDAPRDYALLNTSVNVVDFAADARMATIVSWADVSHLDAPGDDDSFK
                     KREPEPGR"
     gene            complement(1829..2767)
                     /locus_tag="VHV99_29820"
     CDS             complement(1829..2767)
                     /locus_tag="VHV99_29820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEX3639607.1"
                     /translation="MSDQAKQPTSSGGLAQLLTGRIRSKLAAGVTHLTTGSGPTLDYS
                     SPPGDPGLFGPDSVCWKVHADFTSMMTGGISALLLQALHPLALAGVWDHSTFRTDILG
                     RLRRTATFIAGTTYGNRHDALALIERVKHIHLGVTGVGPDGRPYRASDPALLTWVHVA
                     EVSSFMTAHLRYVNPLLSVAAQDQYFAETGRIAEMLGASTIPRSRAEIDAYLLVMQSE
                     LVASERTQEVVRILMNAPAPTAAMRPAGALMLNAGVDLLPDWAQTMLGFERFAALRRS
                     ITRPGVRLVAPVIRWALVNGVSKRARRRVAAAREST"
     gene            complement(2803..3867)
                     /gene="ruvB"
                     /locus_tag="VHV99_29825"
     CDS             complement(2803..3867)
                     /gene="ruvB"
                     /locus_tag="VHV99_29825"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX3639608.1"
                     /translation="MIETDKLAAERIIAATPVSPNEEAFERALRPRQLEEYVGQEKVR
                     GQLEIFIEAAKRRSESLDHVLLFGPPGLGKTTLAHIIAREMGVNLRQTSGPVLERAGD
                     LAALLTNLEANDVLFIDEIHRLSPVVEEILYPALEDYQIDIMIGEGPAARSVKLDLQP
                     FTLVGATTRAGMLTNPLRDRFGIVARLEFYNAEELARIVTRSASLLNAQIHPDGAFEI
                     AKRARGTPRIANRLLRRVRDFAEVKADGNITAQVADAALKMLDVDAVGFDLMDRKLLE
                     AILHKFDGGPVGVDNLAAAIGEERDTIEDVLEPYLIQQGFLQRTPRGRVATLLTYRHF
                     GLAAPDASSPISGLWDAAAT"
     gene            complement(4117..4252)
                     /locus_tag="VHV99_29830"
                     /pseudo
     CDS             complement(4117..4252)
                     /locus_tag="VHV99_29830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190412.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="RuvA C-terminal domain-containing protein"
BASE COUNT          731 a         1362 c         1398 g          761 t
ORIGIN      
        1 actcgcaagc cgacgccgag cgcgcgctcg aagacttcaa ccatcgcgcg aagggcggcg
       61 tgccggacga tatcccggcg gtcacgcttg cgggcgcccc gctcgcaatc ggccagttac
      121 tcaagcaggc caacctcgtg ccgtccacca gcgaggcgct gcgcaacatc gagcagggcg
      181 gcgtgaagat cgatggcacg acggtgtccg acaagggcct caaggtcgaa gccggcgagt
      241 acgtcgtgca ggtcggtaag cgccggtttg cgcgtgtcac gctgacggct tgatgcacag
      301 cacccacgcg atgttgactc aggcgtcgca ttcggagtcg cgccgattat cgcgctgatc
      361 cagcgcgtgc ggcgcgcgga agtgcgcgtg ggcgaccgcg tcaccggcgc gatcgaggcg
      421 ggcctgctcg cgctcgtgtg cgccgaacgc ggcgatacgg aaggcgccgc tgaccggctg
      481 ctcgccaagg tgctcggcta ccgcgtcttc agcgacgcgg ccggcaagat gaatctctcc
      541 gtgcagaatc tcgacggcgc ggggcgcgca ggcggtttgc tgctggtttc gcagttcacc
      601 ttggccgccg acaccaacag cggcttgcgt cccagcttca cgcctgccgc accgcccgat
      661 gagggcaagc ggctgttcga ttacttcgtc tcggcagcgc gcgcaaagca tccgatcgtc
      721 gagacaggcg agttcggcgc cgatatgcag gtgtcgctcg tcaacgacgg gccggtcacg
      781 ttctggctgc agaccaaccc ttgaatttca actgccgccg cgtcgtgtcc gcggcggtac
      841 gcatcgactc atccagccgc tttttgcgac aatagcggct cggcataacc ggctactggc
      901 gcccacgttc atcccatgac cacgcagatt ctgttcatcc gccacggcga aaccgactgg
      961 aaccgcatca agcgcattca aggccacatc gacattccgc tcgcgaccac cggcctcgcg
     1021 caggcacaaa gccttgcaag ccgcatcgcg aatgaggtga aagagggcgc gcggctcgac
     1081 gcgatctatt caagcgatct gctccgcgcg cagcaaaccg cgcagccgat cgccgacgcc
     1141 cttggtctgc ccgtgcaact gagtgagggt ttgcgcgagc gctcatacgg cgcgttccag
     1201 ggacatgaca gcgacgaaat cgccgtccgt ttccccgatg aatacgcgca ttggcaaacc
     1261 cgcgatccag gtttcgcgcc gcctgaaggc gaatcgcagc gcgcgtttta ccaccgcgtg
     1321 ctgcatgcgg tcgagccgat cgtcgctgcg catccgggca gacgggtcgc tgtcgtcgcg
     1381 cacggcggtg tgctcgattg tgtgcgccgt tttgcggcgc gtttgtcgct cgatgcgcca
     1441 cgcgattacg cgttgctgaa cacgagcgtc aacgtggtgg atttcgccgc tgacgcccgc
     1501 atggcgacga tcgtctcgtg ggcggacgtg tcgcatctcg atgcgccggg cgatgacgat
     1561 agtttcaaga agagagagcc agaaccgggg cgttaagcgg ctcggcttcc gcttgcatgg
     1621 agttggtttg tgacggctgc gccgcgcatg acacccccgc ggggtgcgcc ggcacctgca
     1681 cctgcaccgg cggctcgaga tcgggaacgg cgggcatcgc cgatccaatc gcgatgtagc
     1741 catgttcgcc agcaatgctt gatctgacga agctgctcgc ggaagctcct tcggagcgaa
     1801 ttatttcgta gtggctattt cgcgcggctc acgtcgattc gcgcgcggcg gccacgcggc
     1861 ggcgtgcccg cttcgataca ccgttcacga gggcccagcg gatcaccggc gcgaccagcc
     1921 gcacgccggg tcgggtaatc gacctgcgaa gagcggcgaa tcgctcgaag cccagcatgg
     1981 tttgcgccca atcgggcagc agatcgacgc cggcattcaa catcaacgca cctgcgggcc
     2041 gcatcgctgc agtgggtgcc ggcgcgttca tgagaatcct cacgacttcc tgagtacgct
     2101 cgctggccac cagctcggac tgcatgacga gcagataggc gtcgatctcg gcgcgtgagc
     2161 gcggaatagt cgacgcgccg agcatctcgg cgatgcgccc ggtttccgcg aagtattgat
     2221 cctgcgctgc aacggacagc aatgggttca cgtaccgcaa gtgcgcagtc atgaaactcg
     2281 atacctcggc cacgtgcacc catgtcagca aggcagggtc gcttgcacga tatggccggc
     2341 catcaggtcc aacacccgtg acgccgaggt gaatgtgctt cacgcgctcg atcagcgcta
     2401 aagcatcgtg ccggttgcca taggtggtgc cggcgatgaa agtcgcggtt cggcgcaggc
     2461 gcccaagaat atccgtgcga aacgtcgaat ggtcccatac gccggcgagc gccagcgggt
     2521 gcaatgcctg gagcagcaac gcgctgatgc cgcccgtcat catcgacgtg aagtcggcgt
     2581 ggactttcca gcacacggag tcggggccga agagcccggg gtctcccggc ggcgatgagt
     2641 agtcgagtgt ggggccgctg ccggtggtca ggtgcgtgac gcccgcggcc agtttcgagc
     2701 ggatcctgcc cgtgagtaac tgcgcgaggc cgccgcttga agtgggctgc ttggcttgat
     2761 cggacatccc taaagcgatc tcacgcgact ggtatccggg gctcaggttg cggccgcatc
     2821 ccacaggccg gatattgggc tggacgcgtc cggcgcggcg agaccgaagt gccggtacgt
     2881 gagcagcgtg gcgacgcggc cacgcggcgt gcgctgcagg aagccttgct ggatcagata
     2941 cggttcgagc acgtcttcga tcgtgtcgcg ttcttcgccg atggccgccg ccagattgtc
     3001 gacgccgacc gggccgccgt cgaacttgtg gaggatcgct tcgaggagct tgcggtccat
     3061 caggtcgaag ccgaccgcgt ccacgtcgag cattttgagc gcggcgtcag cgacttgcgc
     3121 ggtgatgttg ccgtccgctt ttacctcggc gaaatcgcgc acgcggcgca acagccggtt
     3181 cgcgatccgc ggcgtgccgc gcgcgcgctt ggcgatttcg aacgcgccat ccggatgaat
     3241 ctgcgcattc agcagcgatg cggaacgcgt gacgatgcgg gcaagctctt cggcgttgta
     3301 aaactcgagc cgcgcgacga taccgaagcg atcacgcagc ggattggtca gcatgcccgc
     3361 gcgggtggtt gcgccgacca gtgtgaacgg ctgcagatcc agtttgacgc tgcgcgctgc
     3421 cggcccctcg ccgatcatga tgtcgatctg atagtcctcc aacgccggat acagaatttc
     3481 ttcgacgacc ggtgagagcc ggtggatttc gtcgatgaat aagacgtcgt tggcttcgag
     3541 attggtgagc agcgcggcga ggtcgccagc gcgttcgagc accggaccgg aggtttgccg
     3601 cagattgacg cccatttccc gcgcgatgat gtgtgcgagg gtggtcttgc cgagacccgg
     3661 cggaccgaac agcagcacgt ggtcgagcga ctcggaacgg cgtttggcgg cctcgatgaa
     3721 aatttccagc tgaccgcgca ctttttcctg cccgacatat tcttcgagct ggcgcgggcg
     3781 caacgcgcgt tcgaacgctt cttcgttcgg cgagacgggc gtggccgcga tgatgcgctc
     3841 ggcggcgagt ttgtcggttt cgatcatgcg gccattgtac cgcgcgcgcc ccgttgcgaa
     3901 actcggccga acggccaagg tttgcggccc caagggggcg ttcgaggcgc gcgccacggg
     3961 gacttccttc agggcgccca gtactgggac ttccttcggt gcgcgccaag ggacttcctt
     4021 cggtgcgcgc caagggactc ccttcgctgc gccccccacc gctgacgaag ccatacttat
     4081 cgcacaacac ggcgccgccg cccctggccc cagcttttag cccttcgaca acgccttcaa
     4141 cgcgagcttg atgccttcgg aaacgccggt gccggccggc acgttcttga tgccgccagc
     4201 gcttcttttt cagagtaacc cagcgcgatc agtgcgttca ggatatcgga gg
//
LOCUS       DATASH010001123         7694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5339091,
            whole genome shotgun sequence.
ACCESSION   DATASH010001123 DATASH010000000
VERSION     DATASH010001123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 7694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7694
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5339091"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..880
                     /locus_tag="VHV99_29835"
     CDS             8..880
                     /locus_tag="VHV99_29835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX3639609.1"
                     /translation="MNTMLYPELYKSLESVRWDMEKDIPWDKFDASLLTDEQAATIKM
                     NAITEWSALPATEMFLRDNHHDSDFSAFMSVWFFEEQKHSLVLMEYLRRFKPEMCPTE
                     EELHAVRFEFDPAPPLETLMLHFCGEIRLNHWYRRAAEWHTEPVIKAIYETISRDEAR
                     HGGAYLRYMKKAMVQTGDIARAAFAKIGVLMASARRTEKPLHPTNLHVNQALFPRDTI
                     QSRLPDPEWLERWLDEQIRFDDGWEKKVVERILHNLSILFERTFNTAQELNRYRKEVV
                     ARLQADQKSAEQPA"
     gene            1055..1537
                     /gene="rfaE2"
                     /locus_tag="VHV99_29840"
     CDS             1055..1537
                     /gene="rfaE2"
                     /locus_tag="VHV99_29840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HEX3639610.1"
                     /translation="MAAIFERKIFTRDALVKLRASMPAPVVFTNGVFDILHRGHVTYL
                     ADAKALGACLIVGVNSDASVRMLGKGDDRPINNESDRTALLAALESVDYVVCFGEKTP
                     VELIAALRPDVLVKGGDYDMDALPESAIVRGWGGKALAIPFEHERSTTALLKKVRAQG
                     "
     gene            complement(1541..1780)
                     /locus_tag="VHV99_29845"
     CDS             complement(1541..1780)
                     /locus_tag="VHV99_29845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013337869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639611.1"
                     /translation="MLRWLIAILFLANMLAFVAMRGVFGPTPTAGGREPNHLNRQFHA
                     EWLKVQPVTAAEAADQAVVGGPAPTAPIAASALSQ"
     gene            complement(1834..2649)
                     /locus_tag="VHV99_29850"
     CDS             complement(1834..2649)
                     /locus_tag="VHV99_29850"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEX3639612.1"
                     /translation="MTSGAPYLLIDAGNSRIKWALVQPDGAPIVAGTLTHGGADQPDW
                     SNVPAPDSAWISNVAGESVAARIAALLDARWPHLPRTTIRAGAQQCGVTNRYTTPHAL
                     GSDRWAGLIGAHAAFPGENLLIATFGTATTLEALRADGCFVGGLIAPGWTLMMRSLGE
                     HTAQLPTLDATAARGLLDANTPSERERHGPFFATDTPRSLSAGCTLAQAGLIERMWRD
                     LQDEWQVPVRLVVSGGAVDEVTSALKVPHTRHDSLVLSGLALIAAGRAMERDA"
     gene            complement(2646..3551)
                     /locus_tag="VHV99_29855"
     CDS             complement(2646..3551)
                     /locus_tag="VHV99_29855"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEX3639613.1"
                     /translation="MNASKTPPQVAAASDWRIDRERAVALFGPHAHDWPIEIVEETGS
                     TNADLMTRVKALPRKADALPRPIVRVAYLQTAGRGRRGRPWYAEPGNALLFSVACVLP
                     RPLEGLAGLSLAVGVALVDGLRSLPVAGPGQIALKWPNDVLLEGDKLAGILIETAWST
                     DDASAVVIGIGTNVKGADELAAKVGALNANVPAQARGTVPTALQHALPNANLTDTLAA
                     ELNALEPALQRFGAEGFAPFQARWNAVHAYAGREVVLLEQDRALARGVATGVDELGQL
                     LLNTAAGRLSVATGDVSLRLADGAA"
     gene            3657..4790
                     /locus_tag="VHV99_29860"
     CDS             3657..4790
                     /locus_tag="VHV99_29860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3639614.1"
                     /translation="MNYDTPPGLDVAAGSQGKIVRLSGQWTALALARDKATGHVIPLL
                     RSLIGAEGIRQWDLSRIDRMDHVGGQALWRVWGHKMPPDTTLTDTQRDIFERIALLDT
                     VREKAEPVIRFDPFTRLGLAIFSFFEHLYGGFGMLGQVVIDLFAILRKPKITPWTEIS
                     ANIYNAGTKALPITALVAFLIGIVLSYLSAQQLRLFGANQYIVNILGLSVIRELGPVL
                     SAILVAGRSGSAITAQIGVMRVTEELDAMRVMGIPHGLRLILPRVVALGVAMPLLVMW
                     TNIIALTGGALAAKIVLGIDMSYFARALPSVVPVANLWIGLGKGVAFGMLIAIVGCHF
                     GFRIKANSQSLGEGTTTSVVTSITIVILADAVFAILFQNVGLG"
     gene            4787..5665
                     /locus_tag="VHV99_29865"
     CDS             4787..5665
                     /locus_tag="VHV99_29865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3639615.1"
                     /translation="MIAPLTQAVRGQPMPEIAEPVIEVIDITKRYGRNIVHQHLNFDV
                     RRGEIVSIVGGSGSGKTTLVRQILGLERPSSGTIKLFGEDIATISPETALLMRSRSGM
                     LFQRGALFSSLSVFDNVAQPLRELGKVPEDLLRDIVMLKLEMVGLPCKHASKMPSALS
                     GGMIKRVGIARAIALEPELLFLDEPTAGLDPQASDEFVELISALHRALGLTVVMVTHD
                     LDTMIALSTRVAVLADRKVLVAAPVEEAAGVDHPFIREYFLGLRGRRALQALPPERRA
                     KLPRAALASASSELSL"
     gene            5682..6647
                     /locus_tag="VHV99_29870"
     CDS             5682..6647
                     /locus_tag="VHV99_29870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012399629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX3639616.1"
                     /translation="MENKSHAFWAGIFTVALLAAIAVAAFLFNVDRSVRVPYDLIART
                     NVTGLYTDAAVRYRGLDVGKVQSIKFDRDHPGQILIRILVDTHAPITHSTFGSLGFQG
                     VTGIAFIQLDDNGRDPTPLASSAKQVAQLPMRPGLLDQLQQRGDVLLRKLEKVTDDVD
                     TLLSPEMVSQLHGTAASIQKAADGVATLTQLMAPAAGKLPATINQLDRTLASTNQLIT
                     SLNRPDGPFEMNLNKVGTAAQQAGDALASMDSSIQELSARVGYDTLPRVNSLAEDVRS
                     AARSVDRAADTFSNNPRSVFFGAPSAEPGPGEAGFTWPAAAPAAK"
     gene            6690..7316
                     /locus_tag="VHV99_29875"
     CDS             6690..7316
                     /locus_tag="VHV99_29875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HEX3639617.1"
                     /translation="MSRLTHRLSSSSAALAMLLAFGVLAAGCAGNPAALSNIRYDFGP
                     PNPAASAGTQPAVKVLDISAPDTLESDKLIYRLSYADAQQTASYANSHWTMAPSQLLT
                     QRLRNTLSSRGTVLTGADGVRAPVLKVELSQFEQVFDSQSESHAAVTARATLTQNGKV
                     LGQRTFISRAPSSSADAAGGAQALAAASDDLVSQISAWLGVQALVAAQ"
     gene            7348..>7694
                     /locus_tag="VHV99_29880"
     CDS             7348..>7694
                     /locus_tag="VHV99_29880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teicoplanin resistance protein VanZ"
                     /protein_id="HEX3639618.1"
                     /translation="MSVVKPWQRRPSALARRALLLYTALIVYGSWYPFSGWRSLGLGP
                     FAFLSDPMPQYLTAFDVVTNVLGYMPFGALVVLAVYPRWRGTLAVALAFVLGGLLSGV
                     MEAVQTYLPTRIAS"
BASE COUNT         1301 a         2474 c         2476 g         1443 t
ORIGIN      
        1 tgggactatg aacaccatgc tttatccgga actttataaa tcgctcgaat ccgttcgatg
       61 ggacatggag aaagacattc cctgggacaa gttcgatgca tcgctattga ccgacgagca
      121 ggccgcgacg atcaagatga atgcgatcac cgaatggtcg gcgttgcccg ccacggaaat
      181 gttcctgcgt gacaaccatc acgacagcga tttctccgcg tttatgagcg tgtggttctt
      241 cgaggaacag aagcactcgc tggtgctgat ggaatatctg cgccgcttca agccggaaat
      301 gtgcccgacc gaagaagagc tgcacgcagt gcgcttcgaa ttcgatccag cgcccccgct
      361 cgaaacgctg atgctgcact tctgcggcga aatccgcctg aatcactggt atcgtcgtgc
      421 ggccgaatgg cacaccgagc cggtcatcaa ggccatctac gaaaccattt cgcgcgacga
      481 agcgcgtcat ggcggcgcgt atctgcgtta catgaagaaa gcgatggtgc aaaccggcga
      541 tatcgcgcgt gctgcgttcg ccaagatcgg tgtgctgatg gcatcggcgc gccgcaccga
      601 aaagccgctg catcccacca acctgcacgt gaatcaggcg ctgttcccgc gcgacaccat
      661 tcaatcgcgt ctgccggatc cggaatggct cgaacgctgg ctcgacgagc agatccgttt
      721 cgacgacggc tgggaaaaga aggtggtcga gcggattctg cacaatctgt caattctgtt
      781 cgagcgcacg ttcaatacgg cgcaggaatt gaatcgctat cgcaaggaag tcgtcgcgcg
      841 cttgcaggcc gatcagaagt cggcggaaca gccggcttga tgggttccgg ttcgcgaggc
      901 gaagtctgcg ctttgagtcc gatccggcgc tagcgccgcg aaccgattaa ggtttttaag
      961 aacggcccgg caggcgtgag cttgctgggc cgttttgctt catgccgttc gccgtgtatc
     1021 gtggcggcac ttctattttc ttcgcgactc cgtcatggcc gccatttttg aacgcaagat
     1081 ttttacgcgc gatgcgctgg tgaagcttcg cgcttcgatg cctgcgccgg ttgtatttac
     1141 caacggcgtg ttcgacatcc tgcatcgcgg gcatgtgact tatctcgccg acgcgaaagc
     1201 gctcggggct tgcctgattg tcggcgtcaa tagcgatgct tccgtgcgta tgctgggcaa
     1261 aggggacgac cggcccatca acaacgagtc cgatcggacg gccttgctgg cggcgctgga
     1321 gagcgtcgat tacgtggttt gttttggcga gaagacgccc gtggagctga ttgcggcgct
     1381 gcggccggac gtgcttgtca agggtggcga ctacgacatg gatgcgttgc ccgaatcggc
     1441 cattgtgcgg ggctggggcg gcaaggcttt ggcgattccc ttcgagcatg agcgctccac
     1501 caccgcgtta ctgaagaagg tgcgggcgca ggggtgatct ttactgcgac agcgccgatg
     1561 ccgctatcgg cgctgtcggc gccgggccgc ctactaccgc ctgatcggcg gcttcggctg
     1621 cggtgacggg ctgcaccttc aaccactccg catggaactg gcggttcaga tggttcggct
     1681 cacggccgcc ggctgtgggc gttgggccaa atacgccgcg catcgcgacg aatgccagca
     1741 tgttggcaag aaacaatatg gcgatcagcc agcgcagcat cgttagtatt ccccgtccat
     1801 aagttcagtt gtcgttatcc gtatagtcgc ggctcaggcg tcgcgttcca tcgcgcgtcc
     1861 ggcagcaatc agcgcaagcc ccgacagcac cagcgaatcg tggcgagtat gcggcacttt
     1921 gagcgcactt gtcacttcgt ccaccgcgcc cccgctcacc acgaggcgca ccggcacctg
     1981 ccactcgtcc tgcaagtcgc gccacatgcg ctcgatcagg cccgcttgtg ccaacgtgca
     2041 cccggccgac aacgaccgcg gcgtgtcggt cgcgaagaat gggccgtggc gttcccgctc
     2101 cgacggcgtg ttcgcgtcga gcaagccgcg cgccgccgtg gcgtcgagtg tcggcagttg
     2161 cgccgtgtgc tcgccgagcg agcgcatcat caaggtccag ccaggcgcaa tcaggccgcc
     2221 gacaaagcag ccgtccgcac gcaacgcttc gagcgtggtg gcggtgccga aggtcgcaat
     2281 gagcagattc tcgccgggaa atgccgcatg cgccccgatc aggccggccc agcggtcgct
     2341 gccgagcgca tgcggcgtgg tatagcgatt agtcacgcca cactgctgcg cgccggcgcg
     2401 gatcgtcgtg cgcggcaggt gcggccagcg agcgtcgagg agtgcggcaa tccgcgccgc
     2461 gacactttcg cccgcgacgt tcgatatcca cgcgctgtcc ggagccggca cattcgacca
     2521 gtcaggctgg tcagcgccgc cgtgcgtgag cgttccggct acgattggcg caccgtccgg
     2581 ctgtaccagc gcccacttga tgcggctatt tcccgcgtcg atcaataaat aaggcgcacc
     2641 gctcgtcatg cagcaccgtc ggccagacgc agcgagacgt cgccggtcgc gacgctcaga
     2701 cggcccgccg ccgtgttgag cagcaattgc ccgagctcgt cgacccccgt cgccacgccg
     2761 cgcgccagtg cccggtcttg ttccagcagc acgacttcgc ggcccgcata ggcgtgcacg
     2821 gcgttccagc gtgcctggaa cggcgcgaag ccttccgcgc cgaagcgctg caaggcaggt
     2881 tcgagcgcgt tcagctccgc cgccagcgtg tcggtgaggt tcgcattcgg cagcgcgtgt
     2941 tgcagcgcgg tgggcacggt gccgcgcgct tgcgccggca cgttcgcatt cagcgcgccg
     3001 accttggcgg cgagctcgtc cgcgcccttc acattagtgc cgatgccgat caccacagcg
     3061 cttgcgtcat ccgtgctcca tgcggtttcg atcaggatgc cggccagctt gtcgccttcg
     3121 agcaggacgt cgttgggcca cttcagcgcg atctgtccag ggcccgcgac cggcaacgag
     3181 cgcaatccgt cgaccagcgc cacgcccacc gcgagactca atcccgccag cccttcgagc
     3241 ggacgcggca gcacgcatgc caccgaaaac agcagcgcat tgcccggctc cgcgtaccat
     3301 gggcggcccc gccggccgcg gccggcggtt tgcagatacg cgacccgcac aatcggcctt
     3361 ggcagcgcgt cggctttgcg cggcaatgcc ttgacgcgcg tcatcaggtc ggcgttggtc
     3421 gagccggttt cctcgacgat ttcgatcggc cagtcgtgcg catgcggacc gaatagcgcg
     3481 acggcgcgct cgcggtcgat acgccagtcg gaagccgcgg cgacttgggg tggggtcttg
     3541 gaagcgttca tgtctcatat tgtagcgaca gcgaacgctg cgagcccgcg agcacggttc
     3601 atccgccgcg ccttcgttac aatggcctca atccgataac cagattccag cgatccttga
     3661 actacgacac tccgcccggc ctcgacgtcg cggcaggcag ccaaggcaag atcgtccggc
     3721 tctcgggcca gtggacggcg ctcgcgctcg cgcgcgacaa ggccaccggc cacgtgattc
     3781 cattgttgcg ctcgctgatc ggcgccgaag gcatccgcca gtgggacctg tcgcgtatcg
     3841 accggatgga ccacgtcggc ggtcaggcac tgtggcgcgt gtggggccac aagatgccgc
     3901 cggacactac gctcaccgac acgcagcgcg acattttcga gcgcatcgcg ctgctcgaca
     3961 ccgtgcgtga aaaggccgaa ccggtaatca ggttcgatcc gttcacccgg ttgggtctcg
     4021 cgatcttctc gtttttcgag cacctgtacg gcggattcgg catgctcggg caggtcgtga
     4081 tcgatctgtt tgcgatcctg cgcaaaccga agatcacgcc gtggaccgaa atctccgcca
     4141 acatctataa cgccggcacc aaggcgctgc cgatcaccgc actggtcgcg tttttgatcg
     4201 gcatcgtgtt gagttatctg tcggcacagc agctgcggct ttttggcgcg aaccagtaca
     4261 tcgtcaatat tcttgggctg tcggtgattc gcgaactggg accggttctg tcggcgattc
     4321 tggtggccgg ccgctcgggc tcggcgatta ccgcgcagat cggcgtgatg cgcgtcaccg
     4381 aagaactcga cgccatgcgc gtgatgggca tcccgcatgg gttgcggctg attttgccgc
     4441 gcgtggtcgc gctcggcgtg gccatgccgc tgctggtgat gtggaccaat atcatcgcgc
     4501 tgacgggcgg cgcgctcgcc gcgaagatcg tgctcggcat cgacatgagc tacttcgcac
     4561 gcgcgttgcc gagcgtcgtg ccggtcgcca atctctggat tggcctcggc aagggcgtcg
     4621 cgttcggtat gctgatcgcg atcgtcggct gtcacttcgg cttccgtatc aaggccaatt
     4681 cgcaaagcct gggcgaaggc acgacgacct cggtggtgac gtcgatcacc atcgtgattc
     4741 tcgcggacgc ggtgttcgcg atcctcttcc agaacgtggg gctcggatga tcgcgccact
     4801 cacccaggcc gtgcgcggcc aaccgatgcc cgagatcgcg gagccggtga tcgaggtgat
     4861 cgacatcacc aagcgctacg gccgcaacat cgtgcatcag catctgaact tcgacgtgcg
     4921 gcgcggcgag atcgtctcga tcgtcggcgg atcgggttcg ggcaagacca cgctggtgcg
     4981 gcagattctc gggttggaac ggccgtcgtc gggaaccatc aagctgttcg gcgaggacat
     5041 tgccaccatt tcgccggaaa ccgcgctgct catgcgcagc cgttccggga tgctgtttca
     5101 gcgcggcgcg ctgttctcgt cgctctcggt gttcgacaac gtcgcgcagc cattgcgcga
     5161 actcggcaag gtgcccgagg acctgctgcg cgacatcgtg atgctcaagc tcgagatggt
     5221 cggcctgccg tgcaagcacg cgtcgaagat gccgtcggcg ctctcgggcg gcatgatcaa
     5281 gcgcgtgggc atcgcgcgcg ccatcgcgct cgaacctgag ctgctgtttc tcgacgaacc
     5341 gaccgccggg ctcgatccgc aggcgtccga cgaattcgtc gaactgatct ccgcgctgca
     5401 tcgcgcgctc ggcttgacgg tggtgatggt cacacacgat ctcgatacga tgatcgcgct
     5461 gtccacccgc gtcgccgtgc tggcggatcg caaggtgctg gtcgccgcgc cggtcgagga
     5521 ggcggccggc gtcgatcatc ctttcatccg cgaatatttc ctcggcctgc gcgggcgtcg
     5581 cgcgttgcag gcgctgccgc cggaacgccg cgcgaaattg ccgcgcgcgg cactcgcatc
     5641 ggcatcgtcc gaattgtcgc tgtgaaccag ggaacctgac aatggaaaac aaatcacacg
     5701 ccttctgggc agggatcttc acggtggcct tgctggctgc aatcgcggtg gcggcctttt
     5761 tgttcaacgt cgatcgttcc gtgcgggtgc cgtacgatct gattgcgcgc accaatgtca
     5821 cggggctcta taccgatgcg gcggtgcgct atcgcgggct cgatgtcggc aaggtgcagt
     5881 cgatcaaatt cgatcgggat catcccgggc agattctgat ccgcatcctc gtcgacacgc
     5941 acgcgccgat cacgcactcc acgttcggca gcctcgggtt ccagggcgtg acgggtatcg
     6001 ccttcatcca gctggatgac aatgggcgcg atccgacgcc gctcgcgtcg tcggcgaaac
     6061 aggtcgcgca actaccgatg cggccgggct tgctcgatca gttgcagcag cgcggcgacg
     6121 tcttgctgcg caaactcgag aaagtcaccg acgacgtcga cacgttgttg tcgccggaaa
     6181 tggtcagcca gttgcacggc acggcggcga gcatccagaa agccgccgac ggcgtcgcca
     6241 ccctgaccca gctgatggcg ccggctgccg gcaagctgcc tgccacgatc aaccagctgg
     6301 atcgcacgct agcgtcgacc aatcagttga tcaccagtct gaaccgcccg gacggcccgt
     6361 tcgagatgaa cctgaacaag gtcgggacgg cggcgcagca agcgggcgat gcgctggcgt
     6421 cgatggatag ctcgatccag gagttgtcgg cgcgcgtggg ctacgacacg ttgccgcgcg
     6481 tcaattcgct cgccgaagac gtgcgctcgg cggcccgttc agtggaccgc gccgcggata
     6541 cgttcagcaa caatccgcgc agcgtgttct tcggtgcgcc gagcgcggag ccgggcccag
     6601 gcgaagccgg cttcacatgg cccgccgccg cgcctgcagc taaatgaaat ggtttgaagg
     6661 tttgaaggtt tgaagaaagg aagattgaca tgtcacgctt gactcaccga ttgtcgtcct
     6721 cgagcgccgc gcttgcgatg ctgctcgcct tcggcgtgct ggctgccgga tgcgcaggca
     6781 accccgcggc actctcgaac atccgctatg acttcgggcc gccgaatccg gccgcctcgg
     6841 ccggcacgca gccggcagtg aaggtgctcg acatcagcgc gcccgacacg ctcgagtccg
     6901 acaagctgat ctaccgcctc agctacgcgg acgcgcagca gacggcgtcc tacgcgaaca
     6961 gccactggac catggcgccg tcgcaattgc tgacacagcg gctgcgcaac acgctcagtt
     7021 cgcgcggtac cgtactgacg ggcgccgacg gcgtgcgcgc cccggtgctg aaggtcgaac
     7081 tgagccagtt cgaacaggtc ttcgatagcc agtcggaaag ccacgcggcg gttactgcgc
     7141 gtgccacgtt gacgcaaaac ggcaaggtgc tcggccagcg cacgtttatc tcgcgcgcgc
     7201 cgtccagttc ggcggacgcc gccggcgggg cgcaagcgct tgctgccgcc agcgacgacc
     7261 tcgtctcgca gatcagcgcg tggctcggcg tgcaggcgct ggtcgccgcg caatgaccgg
     7321 catgcagcgc acgcaacgga gcgctgcatg agcgttgtta aaccgtggca gcgtcggcct
     7381 tcggcgctcg cccggcgcgc gctgctgctg tatacggcgc tgatcgtcta cggttcgtgg
     7441 tatccgtttt ccggctggcg ttcgctcggc ctcggtccgt ttgcgtttct gtccgatccc
     7501 atgccgcagt acctgacggc gttcgacgtc gtcaccaacg tgctcggcta tatgccgttc
     7561 ggcgcgctgg tggtgctcgc cgtgtatccg cgctggcgcg gcacgctggc ggtcgcgttg
     7621 gccttcgtgc tcggcgggtt gttgtcgggc gtgatggagg cggtgcaaac ctatctgccg
     7681 acgcgcattg cctc
//
LOCUS       DATASH010001124         2662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3162119,
            whole genome shotgun sequence.
ACCESSION   DATASH010001124 DATASH010000000
VERSION     DATASH010001124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3162119"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..1004
                     /locus_tag="VHV99_29885"
     CDS             213..1004
                     /locus_tag="VHV99_29885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX3639619.1"
                     /translation="MRGRGAQHEFHDNERGRLSQQEFHSADRGGFDHGHRFGHHSHAR
                     FSFHALWHAIGRHHEHRGGGRGGRFGGGPGGFGGDSDGFPRGRKFSSDDLQLLLLSMI
                     DAQPSHGYELIKALETRSNGFYSPSPGMVYPALTYLEELGYVTVQLEGNRKRYELSET
                     GRAYLATNRDRVELMLAKLTHIARKMDSVRRAFAGEEPADISEGGWLPELNEARRALK
                     HALLRRDNAPAAEQRRIAAILMRAANEIEGEGTPGSDASGSEPAA"
     gene            1097..1924
                     /locus_tag="VHV99_29890"
     CDS             1097..1924
                     /locus_tag="VHV99_29890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013343023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-interacting protein"
                     /protein_id="HEX3639620.1"
                     /translation="MLTHDNRADLAVHRVRHPLKFRLLQVKAVRALTPHLIRVTFTGE
                     DLRDFVSASFDDHIKVFFPEPGADKPALPEAGPDGPVFPAGQPRPIARDFTPRRFNAE
                     ARELDIEFAMHEAGPAATWAAQAKVGQYLGIGGPRGSLVIPTGFDWHLLIGDDTALPA
                     IARRLEELPAGTRVAAVIEVADPSAQIEFATRAELHIVWRHRSESGYRGEALLQAVRE
                     MYLPEDGEGYVWAAGESATMRAVRYHLCAERGVDKSRIRAASYWKQGAEAVHESLDD"
     gene            complement(2090..>2662)
                     /locus_tag="VHV99_29895"
     CDS             complement(2090..>2662)
                     /locus_tag="VHV99_29895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX3639621.1"
                     /translation="VPLTLLGVRLAHALPQRVLLALFATVMLVVAGRLLSQALRRGRP
                     DRVDSRFCVGRINPDTGRLIWSWTTGAALAATGALTGLMTGLLGVGGGFIIVPMLRKL
                     TNVSMHGIVATSLMVIALVGSGGVVATVLHGAPLPLDPTLWFSLATGAGMVTGRLLSH
                     RLSARHVQLGFAGVLICVALGMMAKAALGG"
BASE COUNT          504 a          916 c          828 g          414 t
ORIGIN      
        1 tacccaagat gcgtcatccc catcgttttt cccgttcgcg tggtgcggac gacagttttt
       61 tcactgggca taaccgctcg tccctgcatg cgctttggca tgcgttcgcc cgacaatacg
      121 agtcccatgg cgagcgccgc gctgaagcac gtggcgaagg cttcgagcgc cgtcgtggcg
      181 gacgcggcga gtggcgcgag cttcccggcg acatgcgcgg ccgtggcgcc cagcacgagt
      241 tccacgacaa cgagcgtggc cgcctcagcc aacaagaatt tcacagcgcc gaccgcggcg
      301 gcttcgacca cggccatcgc ttcggccacc acagccacgc ccgcttctcg ttccacgcac
      361 tgtggcacgc aatcggccgc catcacgagc atcgtggcgg cggtcgcggc gggcgtttcg
      421 gcggtggccc gggcggcttc ggtggcgaca gcgacggttt tccgcgcggc cgcaaattca
      481 gctccgacga cctgcaactg ctgctgctct cgatgatcga cgcacagccg agccacggtt
      541 acgagttgat caaggcactc gaaacacgct cgaacggttt ctacagcccg agccccggca
      601 tggtctatcc ggcgctcacg tatctggaag aactcggcta tgtgacggtg caactcgaag
      661 gcaaccgcaa acgctacgaa ctatcggaaa cgggccgcgc gtacctggcg accaaccgcg
      721 accgcgtcga attgatgctc gccaaactga cgcatatcgc ccgcaagatg gattccgtgc
      781 gccgcgcgtt tgccggtgaa gaaccggccg atatcagcga aggcggctgg ctgcccgaac
      841 tgaacgaagc gcgccgcgca ctgaagcatg cgctcctgcg ccgtgacaac gcaccggctg
      901 ccgaacagcg ccgcatcgcc gcgatcctga tgcgcgcggc aaatgagatc gaaggcgaag
      961 gcactcccgg cagcgacgct agcggcagcg aaccggctgc ttgagttcgc gctacctgac
     1021 attcgcgcga acactcgcgc gggaaggtgc cgatcaacac cttcgcggca atcaaaccaa
     1081 caggagcagc acccttatgc tgacacatga caaccgagcc gatctggcag tccatcgcgt
     1141 gcggcacccg ctgaaattcc gtctgttgca ggtcaaagcg gtgcgcgcgc taactccgca
     1201 tctgattcgc gtcaccttca cgggtgagga tctgcgcgat ttcgtctccg cttcgttcga
     1261 cgatcacatc aaagtgttct tccccgaacc tggcgccgac aaacccgcgc tgccggaagc
     1321 cggccctgac ggcccggtgt tccccgccgg ccagccgcga ccgatcgcgc gcgatttcac
     1381 gccgcgccgc ttcaacgccg aggcgcgcga actggacatc gagttcgcca tgcacgaggc
     1441 cggcccggct gcaacgtggg cggcgcaagc caaagtgggc cagtatctcg gcattggcgg
     1501 cccgcgcggc tcgctggtga taccaaccgg cttcgactgg catctgttga tcggcgacga
     1561 caccgcgctg ccggccattg ctcgccgcct cgaagagttg cctgccggca cgcgcgtcgc
     1621 ggcggtgatc gaggtggccg atccgtcggc acagatcgaa ttcgcgacgc gggccgaact
     1681 gcatatcgtc tggcgccatc gcagcgagtc gggctatcgc ggcgaagcct tgctgcaagc
     1741 ggtccgcgaa atgtacttgc ccgaagacgg cgaaggctac gtgtgggccg ccggcgaatc
     1801 cgcgacgatg cgcgcggtgc gctaccacct gtgcgcggaa cggggcgtcg acaagtcacg
     1861 tattcgcgcg gccagctact ggaaacaggg ggccgaggcg gtacatgaat cgctcgatga
     1921 ctgaggcagc ttgcgtgcgc gcaatgaacg ccgaagcgca gaccggcgag cacggccctg
     1981 cgcgcctgaa ctcgcctgaa ctcgctcgtc ctgggctctc acctgcttta attagaggtc
     2041 ttaagagcct tggtttatgt cacttgcgcc aaagcgtggc caacgagagt cacccaccta
     2101 gcgcggcttt cgccatcatc ccaagcgcca cgcagatcaa cacgcccgcg aaacccagct
     2161 ggacatgccg cgcggagaga cggtgcgaga gcagccgccc ggtcaccatg ccggcgcccg
     2221 tcgccaggct gaaccacagc gtcgggtcga gcggcaacgg cgcgccgtgc aggaccgtcg
     2281 cgacgacacc gccgctgccg accagcgcta tcaccatcag cgacgtagcg acgatgccgt
     2341 gcatcgacac gttggtcagc ttgcgcagca ttggcacgat gatgaagccg ccgcccaccc
     2401 cgagcagccc cgtcatcagc ccggtcaacg cgccggttgc cgcaagtgcg gcgcccgtgg
     2461 tccatgacca gatcagccgc ccggtgtcgg gattgatgcg gccgacgcaa aaccgtgagt
     2521 ccacgcgatc cggtcgcccc cgccgcaaag cctgtgataa cagccgtccg gccaccacca
     2581 gcatgacggt ggcaaacagc gccaatagca cgcgttgtgg taacgcatgc gcgaggcgca
     2641 caccgagcaa ggtcagtggc ac
//
LOCUS       DATASH010001125         4987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6921429,
            whole genome shotgun sequence.
ACCESSION   DATASH010001125 DATASH010000000
VERSION     DATASH010001125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4987
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6921429"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(799..2157)
                     /gene="glmM"
                     /locus_tag="VHV99_29900"
     CDS             complement(799..2157)
                     /gene="glmM"
                     /locus_tag="VHV99_29900"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004542480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX3639622.1"
                     /translation="MARRYFGTDGIRGTVGEGPITPEFVLRLGYAAGKVLAGADRWAR
                     TGTRPTVLIGKDTRVSGYMLEAALEAGFSAAGVDVMLAGPMPTPGIAYLTRALRLAAG
                     VVISASHNPYYDNGIKFFSADGNKLPDEVESQIEEQLDLPLACAASEQLGKARRLDDA
                     AGRYIEFCKSTFPAAFDLRGLKLVVDCAHGAAYDVAPHVFHELGADVIPIGVAPNGFN
                     INDGVGATAPDALVRAVRANHADLGIALDGDADRLQVVDAAGRLYNGDELLYVLVKDR
                     MATEGNVEGAVGTLMTNMAVEVALQEAGVKFVRAAVGDRYVLEQLREHGWQLGAEGSG
                     HILSLDRHSTGDGIVSALLVLAAMKRSDKTLAELLHGVTLFPQKLINVRMKPGADWKG
                     SDAIRRAISKVEQALNGRGRVLIRASGTEPVLRVMVEAEKVNDAVRYAESIADAVKQA
                     TA"
     gene            complement(2186..3100)
                     /gene="folP"
                     /locus_tag="VHV99_29905"
     CDS             complement(2186..3100)
                     /gene="folP"
                     /locus_tag="VHV99_29905"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX3639623.1"
                     /translation="MIDRYVPKVEFTSLLLPEPLQCGRFKLTFERPLVMGILNVTPDS
                     FSDGGQYAMRGDALRQAERMLLDGADIIDIGGESTRPGAPPVPLDEELERVIPLVEQL
                     RGANVPLSIDTYKPEVMRHALTAGADLINDIWGFRMPGAIDAVRNSECGLCVMHMLGE
                     PQTMQVGEPVYDDVVSEVRQFLEERVAALRQAGIARARISVDPGFGFGKAVVEHNYAL
                     LAHLPDTAPRAEPPYPILAGMSRKSMVGAVVERPASERVAGSIAAAVCAAGRGAAILR
                     VHDVAQTVDALKVWAAMREAANHSRARG"
     gene            complement(3169..>4987)
                     /gene="ftsH"
                     /locus_tag="VHV99_29910"
     CDS             complement(3169..>4987)
                     /gene="ftsH"
                     /locus_tag="VHV99_29910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010102839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX3639624.1"
                     /translation="QFDKPRVQEGVSYSQFMDDAKNGKVKNVIVQGRNLTVTPADGQK
                     YQIVSPGDIWMVGDLMKYGVQVSGKADDEPNALVSALYYLGPTILIIGFWFYMMRQMQ
                     GGGKGGAFSFGKSRARLIDENNNAINFTDVAGCDEAKEEVSELVDFLRDPQKFQKLGG
                     RIPRGVLLVGPPGTGKTLLARAIAGEAKVPFFSISGSDFVEMFVGVGAARVRDMFEQA
                     KKHAPCIVFIDEIDAVGRHRGAGMGGGNDEREQTLNQMLVEMDGFEANSGVIVIAATN
                     RSDVLDKALLRPGRFDRQVYVGLPDIRGREHIMKVHLRKVPISNDVDAAVIARGTPGF
                     SGADLANLVNEAALFAARRGKRIVEMNDFEDAKDKIFMGPERKSAVIREESKRATAYH
                     ESGHAVIAKLLPKADPVHKVTIIPRGRALGVTWQLPEHDNETYSKDYLLDRLAILFGG
                     RVAEELFLNLISTGASDDFNKATATARAMVARFGMTDALGPMVYVDDENDATPFGRGF
                     TRTISEATQQKVDAEIRRVLDEQYNLAKRLLDENRDKVEAMTAALMEWETIDADQIND
                     IMAGRPPRSPKSSPPSASDASSGGSPGTEVKPGSATAPA"
BASE COUNT          874 a         1613 c         1545 g          955 t
ORIGIN      
        1 gctgcgaagg tgctgcccgc gccggtgatg tctgcggctt gcgctgcgat cgcgaaaagc
       61 gcgccagcga cgccagcgaa cacggtttgc atcaatttca tgagacctcc aagggtgtga
      121 gcggtgtgag cgaataacat gaacaccgcg aagcttaggg tctctttgtg acagtaacgt
      181 gactaaccgt gaaacggatg tgacagtttg attaccaagt tgaaaggcgg tcgaacgaac
      241 gaattgctgg tttagcgaag ttgcgggcgt cgccctacag attaggcgaa aaagatgggg
      301 cgcgaacgtt tgcgcggggg atggctgcgg tgggggatgg ctgcggtggg ggatggctgc
      361 ggtggagggt cggtgcggtg gaggatcggt gcggcggggg ttggtgcggc gggaaatttt
      421 taccggcttg cggacgaggc tagtcagact aaggggcagg atggatgttt gaaaatgact
      481 ggctgggcag aagtcgggcg gcgggggaca ttccaagcgc ggtttgtcgc cgctcgggct
      541 gaggcggggc gatctgcttt accgcccgtc gcgacctacc cacagtggga tgatccgctt
      601 gccgtccggc gccgcccatc tacggtaggg acgatctgct tgccgtccag cgccgcccgc
      661 ccacggtggg gacgatctgc tggccgtccg tcgccgccgg tccacacggt gaggacgatc
      721 cgctggctgt ccgtcgccgc cgatccaggt ggggacgatc tgcgtgccgt ccgccgcctc
      781 gatcacccgc gtgattatct aagccgtagc ttgcttgacc gcgtcggcga tcgattcggc
      841 ataccgaacc gcatcgttga ctttttccgc ctcgaccatc acacgcagca cgggctcggt
      901 gccggatgcg cggatcaaca cgcggccgcg gccgttcagc gcctgctcaa ccttgctgat
      961 ggcgcgacgg atcgcgtcgc tgcccttcca gtccgcgccg ggcttcattc gcacgttgat
     1021 cagcttctgc ggaaacagcg tcacgccgtg gagcaactcg gcgagcgttt tgtcgctgcg
     1081 cttcatcgcg gccagcacca gcagggccga gacgatgccg tcgccggtcg aatggcggtc
     1141 gagcgagaga atatggccgg agccttccgc gcccagttgc cagccgtgct cacgcaactg
     1201 ctcgagcacg tagcggtcgc ccaccgccgc ccggacgaat ttcacgccgg cttcctgcag
     1261 cgcgacttcg accgccatgt tggtcatcaa ggtgccgacc gcgccttcca cgttgccctc
     1321 ggtcgccatc cggtccttga ccagtacgta cagcaactcg tcgccgttat acaggcgacc
     1381 cgctgcatcc acgacctgta gccggtcggc atcgccgtcc agcgcaatgc cgaggtccgc
     1441 atgattcgcg cgcaccgcgc gcaccagcgc gtccggagcg gttgcgccga caccgtcgtt
     1501 gatgttgaaa ccgttcggcg cgacgccgat cggaatcacg tcggcgccga gttcgtggaa
     1561 cacgtgcggc gcgacgtcgt acgcagcgcc atgcgcgcag tcgaccacca gcttgaggcc
     1621 gcgcaagtcg aaggcggccg ggaaggtgct cttgcagaac tcgatgtaac ggccggccgc
     1681 gtcgtcgagg cgtcgcgcct tgccgagttg ttcggatgcc gcgcacgcaa gcggcagatc
     1741 gagctgctct tcgatctgcg attccacttc gtcgggcagc ttgttgccat cggcggagaa
     1801 gaatttgatg ccgttgtcgt agtacgggtt gtgcgacgca ctgatcacga cgcccgcggc
     1861 cagccgcagt gcgcgcgtca gataggcgat gcccggggtc ggcatcggcc ccgccagcat
     1921 gacgtcgacg ccggccgcgg aaaagcccgc ttcgagcgcg gcttcgagca tatagcccga
     1981 cacccgcgtg tctttaccaa tcagcaccgt gggccgcgtg cccgttctgg cccagcggtc
     2041 cgcgcccgcg agcaccttgc cggcagcgta gccgagccgc aatacaaact ccggcgtaat
     2101 cggcccttcg ccaaccgtgc cccgaattcc gtccgttccg aaatagcgac gtgccatgtt
     2161 ttatagtcct ccttcatttt tggccttagc cgcgcgcgcg gctgtgattc gccgcctcgc
     2221 gcatggctgc ccatactttc aatgcatcta ctgtctgcgc gacgtcgtgc acgcgcagaa
     2281 ttgccgcgcc gcgcccggca gcacacactg ccgcggcgat gctgcccgcg acgcgttccg
     2341 acgccggccg ttccaccact gcgccgacca tcgacttgcg cgacatgccc gcaagaatgg
     2401 ggtacggcgg ctcggcccgc ggcgccgtgt ccggcaggtg cgcgagcagc gcgtaattgt
     2461 gttcgaccac cgccttgcca aagccaaaac ccggatccac gctgatgcga gcacgggcga
     2521 tgcccgcttg cctcagcgcc gccacacgtt cttcgaggaa ctgtcgaacc tcgcttacga
     2581 cgtcgtcgta gacgggctcg ccaacctgca tggtctgcgg ctcgccgagc atgtgcatca
     2641 cgcatagacc gcattcgctg ttgcgcacgg cgtcaatcgc accgggcatc cggaagcccc
     2701 agatatcatt gatcaggtcg gcacccgccg tcagtgcgtg acgcatcact tccggcttgt
     2761 atgtatcaat cgacagcggc acatttgcgc cgcgcagttg ttcgaccagc gggatcacgc
     2821 gctccagttc ttcgtcgagc ggcaccggcg gcgcacccgg gcgggtcgac tcaccgccga
     2881 tgtcgatgat atccgcgccg tcgagcaaca tgcgctcggc ctgccgcagc gcatcgccgc
     2941 gcatggcgta ctgaccgccg tcggagaaag aatcgggtgt gacgttcagg atgcccatga
     3001 ccaacgggcg ttcaaaggtc agcttgaagc ggccgcattg cagcggttca gggaggagaa
     3061 gagaagtaaa ttcgactttc ggcacgtagc ggtcaatcat gtaaaacgga taaatgcaaa
     3121 acgggccggt gtgaaacaca ccggcccgca ctttctactg gtcgccgatc aggccggcgc
     3181 ggtcgcgctg cccggcttga cctcggtgcc cgggctgcca cccgacgagg cgtcgcttgc
     3241 cgacggcggc gagctcttcg gcgaacgcgg cggacggcct gccatgatgt cgttgatctg
     3301 atcggcgtcg atcgtttccc actccatcag cgcggcggtc atcgcttcga ccttgtcgcg
     3361 gttctcatcc agcaagcgct tggcgaggtt gtattgctcg tccagcacgc ggcggatttc
     3421 cgcgtcgacc ttctgctgtg ttgcttccga aatggtgcgc gtgaagccac ggccaaacgg
     3481 cgtagcgtcg ttttcgtcgt cgacgtagac catcggtccg agcgcgtccg tcataccgaa
     3541 gcgagccacc atggcgcggg ccgtagccgt tgccttgttg aagtcgtccg atgcgccggt
     3601 gctgatcagg ttcaggaaca gctcttccgc aacacggcca ccgaacagga tcgccaggcg
     3661 gtccagcagg tagtccttcg aatacgtttc gttgtcgtgc tccggcaact gccacgtgac
     3721 gcccagcgca cggccgcgcg ggatgattgt gaccttgtgc accggatcgg ccttcggcaa
     3781 cagcttggcg atgaccgcgt gacccgactc gtgatacgcc gtagcacgct tcgattcctc
     3841 gcgaatcacg gccgacttgc gctccggacc catgaagatc ttgtccttcg cgtcttcgaa
     3901 gtcgttcatt tcgacgatgc gcttgccacg gcgagccgcg aacagcgctg cttcgttgac
     3961 caggttcgcc agatcggcgc ccgagaagcc cggcgtgccg cgtgcaataa ccgctgcgtc
     4021 gacgtcgttc gaaatcggta ccttgcgcag gtgaaccttc atgatgtgtt cgcggccgcg
     4081 gatgtccggc agaccgacat acacctgacg gtcgaaacgg cctggacgca gcaacgcctt
     4141 gtccagcacg tccgaacggt tcgtggcagc gatcacgatc acaccggaat tcgcttcgaa
     4201 gccgtccatc tcgaccagca tctggttcaa ggtctgttcg cgttcgtcgt taccaccgcc
     4261 catgccggca ccgcgatgac ggccgaccgc gtcgatttcg tcgatgaaca cgatacacgg
     4321 cgcatgcttc ttggcctgct cgaacatatc gcgcacacga gccgcaccga cacccacgaa
     4381 catttccacg aagtccgaac ccgaaatgct gaagaacggc accttcgctt cgccagcgat
     4441 agcgcgcgcc agcaacgttt taccggttcc cggcgggccg accagcaaca cgccgcgtgg
     4501 aatgcgcccg ccgagcttct ggaacttctg cggatcgcgc aggaagtcga ccagttcgga
     4561 gacttcttcc ttggcttcgt cgcaaccggc gacgtcggtg aaattgattg cgttgttgtt
     4621 ttcgtcaatc agacgtgcac gggatttacc gaacgagaac gcaccgcctt tcccgcctcc
     4681 ctgcatctgt cgcatcatgt agaaccagaa tccgatgatc aggatcgttg gtccgaggta
     4741 gtacagcgcg gacaccagcg cattcggttc gtcatcagcc ttgccgctca cctgaacgcc
     4801 atacttcatc agatcgccga ccatccagat gtcgccgggc gacacaatct ggtacttctg
     4861 gccgtctgct ggagtgaccg tgaggttccg cccctggaca atgacgttct tgactttgcc
     4921 gttcttcgcg tcgtccatga actgcgaata ggaaacacct tcctggacac ggggcttgtc
     4981 gaactgc
//
LOCUS       DATASH010001126         4316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5538412,
            whole genome shotgun sequence.
ACCESSION   DATASH010001126 DATASH010000000
VERSION     DATASH010001126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4316
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5538412"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VHV99_29915"
     CDS             complement(<1..560)
                     /locus_tag="VHV99_29915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014971793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CobT"
                     /protein_id="HEX3639625.1"
                     /translation="MNARHTSHAARDTRGFAFESTLSKVARVLTGQYGVTVAFSPDGP
                     RVEPGRIVIPDYELSSGVERDVLIGYLDLLVARAKHSSLAQIDALPRGVAANLAQVIE
                     DRRVCAQLLDEYPGARWFIGKLRVHAAEQARQRWPRLHWRDRLVWLVERALWDEQPSL
                     TEASHSLLAALHAAQDLLDEARSSRST"
     gene            complement(596..1822)
                     /locus_tag="VHV99_29920"
     CDS             complement(596..1822)
                     /locus_tag="VHV99_29920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3639626.1"
                     /translation="MNAPELTEDEYLGLYQRATENGAEWWRVSVADRPENYRLEHGVD
                     DSNGAGSVDIDLVVDGENLRAKLKKWRREGFAIDTPDAASEAGAAGRMAFMPELQRLA
                     ARAAAAKSRLQIAAPAETVRIGHVDVPCGVDNPLVPRINPAYLFSERFNDIVEDIVEN
                     RRVMLIGHTGAGKTSLIEQVAARSHHGVLRSNMNGQTTVGDFVGFWTVKGGETIWVDG
                     VLPTAMREGLWLIVDEIDFAEPSILAALTAVLEPHGRLVLKEKGNEIVAPHPAFRLFA
                     TANAVGAMSQFRHLYQGSNLMNEAFLDRWRVYMLDYLTPAEEADVLMGTLAPHMTRAL
                     ATTLAAIAADCRAAFAREDLSSAFSTRRLLDWAELMLRTGDPERAAGPAIYAKVSPED
                     AALIRGIVRHHIAPAG"
     gene            1928..2193
                     /locus_tag="VHV99_29925"
                     /pseudo
     CDS             1928..2193
                     /locus_tag="VHV99_29925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019255475.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2328..>4316)
                     /locus_tag="VHV99_29930"
     CDS             complement(2328..>4316)
                     /locus_tag="VHV99_29930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HEX3639627.1"
                     /translation="RGRLTAPMRWDAATDRYVPTTWQQAFAEIGKELRALDPNEAVFY
                     ASGRASLETSYMYALLARMYGTNNLPDSSNMCHESTSVGLPKSIGAPVGTVQLEDFEH
                     TDCMLFFGHNTGTNAPRMLHQLQDARKRGVEIITFNPIRERGLVSFANPQSPLEMLTP
                     KETQISTQYHQLKIGGDAAAIAGLCKLLIEWDDEAQRDGTQRVLDAGFIAEHTHGFEA
                     FAASMRATTWEEIERHSQLTRAALEAAARVYAQANAAMVLYGMGITQHRSGVNNVQML
                     SNLLLLRGNIGRPGAGICPIRGHSNVQGQRTVGITEKPELAPLDKLKELYGFEPPREK
                     GMTTVEACQGVLDGKVKAFIGLGGNFQVAIPDHHVMDPAWRHLRLTVQIATKLNRSHL
                     LHGEVAYLLPCLGRIEIDAQASGEQWVSVEDSTACVHGSHGMVEPASDMLLSEPAIVA
                     GIAKALLPGNPKVDWDAWVGDYALVRDAIERTYPDQFQDFNQRFRQPGGFHRPLPACK
                     REWKTENGKANFIVPDTLDEDADMPSTGPDVLRLMTTRGDSQFNTTVYALDDRFRGVY
                     GTRDVLLMHQDDLMRLGLAEGDAVCISTVFNDGVTREVDGMRVHAFDIPSGCIMGYYP
                     ECNRLIPLSHHAKSSQVPASKSIPVRMRKSTVVEGAAA"
BASE COUNT          748 a         1380 c         1348 g          840 t
ORIGIN      
        1 gtcgaacggc tgctgcgcgc ctcgtccagc agatcctgcg cggcgtgcag cgcggccagc
       61 agtgaatggc tcgcttcggt caagctcggt tgctcgtccc atagcgcgcg ctccaccagc
      121 cacaccagcc ggtcgcgcca gtgcagcctg ggccagcgct ggcgcgcctg ctcggcggca
      181 tgcacacgca gtttgccgat gaaccagcgc gcgcccggat attcatcgag caattgcgcg
      241 cacacgcggc ggtcttcgat cacttgcgcg agattcgccg cgacgccgcg cggcagcgcg
      301 tcgatctgcg cgagcgacga atgtttggcg cgcgccacca gcagatccag ataaccgatc
      361 agcacgtcgc gctcgacgcc gctgctcaac tcatagtcgg gaatcacgat gcgcccgggt
      421 tccacccgcg gcccgtccgg actgaaggcg accgtcacgc cgtattgccc ggtcagcacg
      481 cgcgccactt tgctgagcgt cgattcgaac gcgaagcctc gcgtgtcgcg cgctgcatgc
      541 gatgtgtgcc tggcgttcat tgtgcttggg gtgcccggta tgctcactgg ccgcgtcagc
      601 cggccggcgc gatgtgatgg cgaacgatgc cgcgaatcag cgccgcgtct tccggactga
      661 ccttcgcgta aatcgcggga ccggcggcgc gttcggggtc gccggtgcgc agcatcagct
      721 cggcccagtc gagcaggcgc cgcgtcgaga atgcactcga caggtcttcg cgggcgaatg
      781 ccgcgcggca gtcggcggca atcgcggcga gggtggtggc gagcgcgcgt gtcatatgcg
      841 gggcgagggt gcccatcagc acgtcggctt cttcggcggg cgtcagatag tccagcatgt
      901 agacgcgcca gcgatcgaga aacgcctcgt tcatcagatt cgagccctga tagagatgac
      961 ggaactggct catcgcaccg accgcattcg ccgtggcgaa cagcctgaac gcgggatgcg
     1021 gcgcgacgat ctcattgcct ttctctttca gcacgaggcg gccgtgcggt tcgagcacgg
     1081 cggtgagcgc ggcgagaatc gacggttcgg cgaagtcgat ttcgtcgacg atcagccaca
     1141 agccttcacg catggccgtg ggcagtacgc cgtccaccca gatcgtctcg ccacccttga
     1201 ccgtccagaa cccgacaaaa tcgccgaccg tggtttgccc attcatattc gagcgcaaca
     1261 cgccgtggtg cgaacgcgcg gcgacctgtt cgatcaggct ggtcttgccg gcgccagtgt
     1321 gaccgatcag catgacgcgc cggttctcga cgatatcctc gacgatgtcg ttaaagcgct
     1381 cggagaacag ataggcggga ttgatgcgcg gcacgagcgg attgtcgacg ccgcaaggca
     1441 cgtcgacgtg accgatgcgc acggtttcgg ccggggcagc tatctgcaga cggctcttcg
     1501 ccgccgcggc ccgcgccgcg aggcgctgca actcgggcat gaacgccatc cgtccggccg
     1561 cacccgcttc gctcgcggcg tcgggcgtat cgatcgcgaa gccttcgcgg cgccacttct
     1621 tcagcttggc gcgcaggttt tccccgtcga ccaccagatc gatatcgact gatccggcgc
     1681 cgttgctgtc atcgacacca tgctccagcc gatagttttc cggccggtcc gccacgctga
     1741 cgcgccacca ttccgcgccg ttttcggtcg cgcgttggta caggcccagg tattcgtctt
     1801 cggtcaattc tggcgcgttc atggcgagtg gcaaatgatg agtggtcaaa aaacaatgaa
     1861 tgctgtttcg atttggctga ggccgagtga ccgtctattt tatcgtgtgt acctttcgat
     1921 cgcctggcag agcatctagg atgaactgcg cgacgccaat ggccgcttta gcaacatctt
     1981 ccaggcgtcg cgtgaatatt tcgtcggcgc caatgtgcat ttcgacgcgg tgaagatcca
     2041 gcactttcgt acggcacggt gggttacgcg cgcaacggca gcaactcgaa tttttcgctt
     2101 gaagcgtcgc cgcgcgatgc cgcgaataac acgagtccgc tcgcgggaga atcgcaaacc
     2161 ggcgcgtatg tggggatgct gcatcagttt tgacgtggcg aataccgcgc aacggccggc
     2221 ggtacattcc ggacgcgggt agcaacaaaa aggcgcagcg gctttaaacc gctgcgcctt
     2281 tttcactgcc gggtcggcgc gggcacactg tcgtgctgtt cccgcactca ggctgcggct
     2341 ccttcgacca ccgtcgactt gcgcatacgc accggaatcg atttcgacgc cggcacctga
     2401 ctcgatttcg cgtgatgcga gagcgggatc aggcggttgc attcggggta gtagcccatg
     2461 atgcaaccgc ttggaatatc aaatgcatgc acgcgcatgc catccacttc gcgcgtcacg
     2521 ccgtcgttga agacggtcga gatgcacaca gcgtcgccct ccgcaagtcc gaggcgcatc
     2581 aagtcgtcct gatgcatcag cagcacgtcg cgcgtgccgt acacgccgcg aaaacggtcg
     2641 tcgagtgcat agacggtcgt attgaactgg ctgtctccac gcgtcgtcat caggcgcagc
     2701 acatcggggc cggtcgacgg catgtccgca tcttcatcga gcgtatcagg cacgatgaaa
     2761 ttggccttgc cgttttccgt tttccattcg cgcttgcagg cgggcagtgg ccggtgaaag
     2821 ccaccaggct gtctgaagcg ctggttgaaa tcttgaaact ggtcgggata agtgcgctcg
     2881 atggcgtcgc gcacgagcgc atagtcgccg acccaggcat cccaatccac cttcggattg
     2941 cctggcaata gcgccttggc gatgcccgca acgatcgccg gctccgacaa cagcatgtcg
     3001 ctggcgggct cgaccatccc gtgcgaacca tgtacgcacg ccgtactgtc ctccacactg
     3061 acccactgtt cgccgctcgc ctgcgcgtcg atttcaatgc gcccaaggca cggcagcaaa
     3121 tacgcaacct cgccgtgcag caggtggctg cggttgagct tggtggcgat ctgcacggtg
     3181 agcctgaggt gccgccacgc cgggtccatc acgtgatgat cgggaatcgc cacctgaaaa
     3241 ttcccgccga gaccgatgaa cgccttgacc ttgccgtcga gcacgccctg gcaagcttcg
     3301 acggtggtca tgcctttctc gcgcggcggc tcgaagccat agagttcttt cagcttgtcg
     3361 agcggcgcga gttcgggctt ttcggtaatg ccgaccgtgc gctgtccttg cacattcgaa
     3421 tgaccgcgaa tcggacagat gccggcgccg ggccgcccaa tattgccgcg cagtagcagg
     3481 agattcgaca gcatctgcac attgttcaca ccgctgcgat gttgcgtgat ccccatgccg
     3541 tacagcacca tcgcggcgtt cgcttgcgcg tacacacgtg ccgccgcttc gagcgccgcg
     3601 cgcgtcagct gcgagtggcg ttcgatctct tcccacgttg tcgcgcgcat ggacgcggcg
     3661 aacgcctcga agccgtgcgt atgttcggcg atgaagcccg catcgagcac ccgctgcgtg
     3721 ccgtcgcgtt gcgcctcgtc gtcccattcg atcagcagtt tgcacagtcc ggcaatcgct
     3781 gccgcatcgc cgccgatctt cagctgatgg tattgcgtgc tgatctgcgt ttctttcggc
     3841 gtgagcattt caagcggcga ctgcggattg gcgaagctga cgagcccacg ctccctgatc
     3901 ggattgaaag tgatgatttc cacgccgcgc ttacgcgcat cctgcaactg atgcaacatg
     3961 cgcggcgcgt tcgtgccggt gttgtggccg aagaacagca tgcaatccgt gtgttcgaag
     4021 tcttcgagtt gcacggtgcc gaccggcgcg ccgatgctct tcggcaagcc gaccgaggtg
     4081 ctctcgtgac acatgttcga gctgtcgggc agattgttgg tgccgtacat gcgggcgagc
     4141 agggcataca tgtacgaggt ttccagcgac gcacgccccg acgcatagaa caccgcttcg
     4201 ttcggatcca gcgcgcgcag ttctttgccg atttcggcga atgcctgttg ccaggtcgtg
     4261 ggcacgtaac gatcggtggc ggcgtcccag cgcatcggcg cggtcaagcg gccgcg
//
LOCUS       DATASH010001127         3249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_3557556,
            whole genome shotgun sequence.
ACCESSION   DATASH010001127 DATASH010000000
VERSION     DATASH010001127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3557556"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1114
                     /locus_tag="VHV99_29935"
     CDS             <1..1114
                     /locus_tag="VHV99_29935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEX3639628.1"
                     /translation="YEREEYAGYRRVNAWLAHKLIKLLEPDDVIWIHDYHLIPFAEAL
                     RAAGITNRIGFFLHIPFPAPQILLNIPPHEELVKSLSCYDLLGFQTATDELAFHDYIT
                     RHARGTVSGGNHVEAFGRKLRTGVYRIGVFPDEIAEQAQRYESRQHVLDLKQSLEGRK
                     LIMSVDRLDYSKGLVERFRAFEQLLERSPEWRGNVTLVQIAPPTRSDVASYQKIRQDL
                     EYEAGRINGRYSGLDYTPIRYLNQQYDRWKLMSLFRESQIGFVTPLHDGMNLVAKEYV
                     AAQNPDDPGVLVLSIFAGAAAELSGALLVNPHDAIGMCEALQRALQMPLDARQRRHEI
                     NMAALRKNDLGVWRDTFLNDLRNVPVRQPGKGGGHK"
     gene            1182..2348
                     /locus_tag="VHV99_29940"
     CDS             1182..2348
                     /locus_tag="VHV99_29940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3639629.1"
                     /translation="MLRTLAIAGYRSLRELIVPLEQLNVITGANGSGKSSVYRSLRLL
                     AETARGGVITSLAREGGLQSTLWAGPERFSRAVLAGELPVEPLRRKEPVSLRLGFSGD
                     EFGYAIDLGLPPLDRATRFPLDPTIKRECIWSGPVLRPSALLVDRHVAALRTRDEQGD
                     WQTIPQPVASFDSMMTEFSDPRTAPEMITVREQIRSWRFYDHFRTDADAAVRLPQIGT
                     HTPVLSNDGADLAAALQTIREIGDPVALDAAIDDAFPGSRLDIETQDGRFEVSMQQHG
                     LLRPLKGAELSDGTLRYLLLVAALLTPRPPALLVLNEPETSLHPDLLPALARLIARAS
                     RHSQVLVVSHAARLIAALEREDGSQSIVLEKHFGATRIADADDRDLPAWKWPTR"
     gene            2846..>3249
                     /locus_tag="VHV99_29945"
     CDS             2846..>3249
                     /locus_tag="VHV99_29945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3639630.1"
                     /translation="MKIAQIAPLTESVPPKLYGGTERVVSYITEALVDLGHDVTLFAS
                     GDSVTSAKLEAVWPRALRLDPGIRDRVAPHMLLMELVRRQAENFDVLHFHLDYYSFSV
                     FKRQETPFVTTMHGRLDLPEQQPVFDTFNTAPV"
BASE COUNT          615 a         1042 c         1010 g          582 t
ORIGIN      
        1 ctacgagcgc gaagaatacg caggctaccg gcgcgtcaac gcatggctcg cgcacaagct
       61 gatcaagctg ctcgaacccg acgacgtgat ctggattcac gactaccatc tgatcccgtt
      121 cgccgaagcg ctgcgcgccg ccggcatcac gaaccgcatc ggcttcttcc tgcacattcc
      181 gtttcccgca ccgcagattc tgctcaacat tccgccacac gaagagctgg tgaaatcgct
      241 gtcgtgctac gacctgctgg gcttccagac cgcgaccgat gaactcgctt tccacgacta
      301 catcacgcgc cacgcgcgcg gcaccgtgag cggcggcaat cacgtcgagg ccttcggccg
      361 caagctgcgc acgggcgtct atcggatcgg cgtgttcccc gatgaaatcg ccgagcaggc
      421 gcaacgctac gaaagccgcc agcacgtgct cgacctgaag cagagcctcg aaggccgcaa
      481 gctgatcatg agcgtcgacc ggctcgatta ttcgaagggg ctcgtcgaac gttttcgtgc
      541 cttcgaacag ctgctggaac gctcgcccga atggcgcggc aatgtcacgc tggtgcagat
      601 cgcgccgcct acccggtcgg acgtcgccag ctatcagaag atccggcagg atctcgaata
      661 cgaagcgggg cgcatcaacg gccgctattc gggcctcgac tacacgccga tccgctatct
      721 gaatcaacag tacgaccgct ggaagctgat gtcgctgttt cgcgagtcgc agatcggttt
      781 tgttacgccg ctgcacgacg ggatgaacct ggtcgcaaag gaatatgtcg ccgcgcaaaa
      841 tcccgacgat ccgggcgtgc tagtgttgtc gattttcgcg ggcgcggcgg cggagttgag
      901 cggcgcgctg ctggtcaatc cacacgatgc gatcggcatg tgcgaagcct tgcagcgcgc
      961 gctgcaaatg ccgctcgatg cgcgccagcg gcggcacgag atcaacatgg cggcgctgcg
     1021 caagaacgat cttggcgtgt ggcgcgatac ctttctgaac gatttgcgca atgtgcccgt
     1081 gcggcagccg ggcaagggcg gcgggcataa gtgatgatgc gtttgaacga agtggtttta
     1141 ccgggcgcgc ggcagtgcgc gtttgaccga acggcggccg catgctgagg accttggcca
     1201 tcgcgggcta tcggtcgcta cgcgagttga tcgtgccgct cgagcagttg aatgtgatca
     1261 cgggtgcgaa tggcagcggt aaatcgagtg tgtatcgctc gctgcggttg ctggctgaga
     1321 ccgcgcgcgg cggcgtgatt acgtcgcttg cgcgcgaggg cggtttgcaa tccacgttgt
     1381 gggccggacc cgaacgcttt tcgcgcgcgg ttttggccgg tgagttgcca gttgaaccgc
     1441 tgcgccgcaa agagccggtg agtctgcggc tcggcttttc cggcgatgaa ttcggctatg
     1501 cgatcgatct gggattgccg ccgctcgatc gagccacccg gttcccgctc gaccccacga
     1561 tcaaacgcga atgcatctgg agcgggccgg tgctgcgtcc gtcggccttg ctggtcgacc
     1621 ggcacgtcgc cgcgctgcgc acgcgcgacg agcagggcga ctggcaaacc atcccacaac
     1681 ccgtcgccag tttcgacagc atgatgacgg agttctccga cccgcgcacc gcccccgaga
     1741 tgatcacagt gcgcgagcag attcgctcgt ggcgctttta tgaccacttc cgcacggacg
     1801 ccgacgcggc cgtgcgtctg ccgcagatcg gcacgcatac gccggtgctc tcgaacgacg
     1861 gcgcggatct cgccgcggcc ttgcagacca tccgcgagat cggcgacccc gtcgcactcg
     1921 atgccgccat cgacgacgcc tttcccggct cgcgcctcga catcgagacg caggacggcc
     1981 gtttcgaagt gtcgatgcag caacacggtc tgctgcggcc gctgaagggc gcggaactgt
     2041 cggacggtac cttgcgctat ctgctgctgg tggccgcgct gttgacgccg cgtccgcccg
     2101 cgctgctggt gctgaacgaa ccggagacga gcttgcatcc ggacctgctg ccggcgctcg
     2161 ctcggctcat cgcgcgggct tcgaggcatt cgcaggtgct ggtggtgtcg cacgcggcac
     2221 ggctgatcgc ggcgctcgag cgcgaggacg gcagccagtc gatcgtgctc gaaaagcact
     2281 tcggcgcgac gcgcatcgcc gatgcggacg atcgtgacct gccggcctgg aaatggccga
     2341 cgcggtaggc atggcttggt gcggtgtcag gacggaggca agcaacgcat ctaagcgacg
     2401 cgcctaagcc acgcgttaaa ccacgcgtta aaccacgcac ctaaaccaca tacctaaacc
     2461 acgtagctaa gccacatacc taagccatgt acctaagcca tgtacctaag ccacgtacct
     2521 aagccacgta cctaagccac gtacctagcc acgtagtaga gcgaagtgcg acgtaccaag
     2581 cgacgtacca agcaatctac ccaagcgacc cactgagaga acaaagccgt cccccaccgt
     2641 atgtaagctg gatgtaacat cgcacaggaa ttgcaagggc aagcgctttt ttattgcgct
     2701 gtggtgaata atgcttcgac gcgagtggcg caggcagacg cgaggcgtcg cctgacatgg
     2761 attgagaagg actggcgcgg cctgtgctca gcgccgctga cgcgcaccgc caccggcgca
     2821 agccgtcagt tcacggagac aaaatatgaa gattgcgcag atcgccccct tgaccgagtc
     2881 ggtgccgccc aagctgtatg gcggcacgga gcgcgtcgtg tcctacatca ccgaggcgtt
     2941 ggtcgacctc ggacacgacg tgacgctgtt cgcgagcggc gattccgtca ccagcgcgaa
     3001 gctcgaagcc gtctggccgc gcgcgctgcg tctcgacccg ggcattcgcg accgcgtggc
     3061 gcctcatatg ctgctgatgg aactggtgcg ccgccaggcc gaaaatttcg acgtcctgca
     3121 ttttcacctc gactattact cgttctcggt cttcaagcgc caggaaacgc ctttcgtcac
     3181 gacgatgcat ggccgtctcg acctgcccga gcagcagccc gtattcgaca cgttcaatac
     3241 cgcgccggt
//
LOCUS       DATASH010001128         4465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4149880,
            whole genome shotgun sequence.
ACCESSION   DATASH010001128 DATASH010000000
VERSION     DATASH010001128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4149880"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="VHV99_29950"
     CDS             complement(<1..647)
                     /locus_tag="VHV99_29950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX3639631.1"
                     /translation="MEANRIIAPRRGPLRTVIDRFRAFGRRAPSKPQSGASRTAQLEQ
                     VVGAVDLLALVDQTLRFLYVSDASLRFIGYHREYLETITLHDLVAPADVPRLDALLKR
                     ATATGNVEKATLGLIKSLTYPITVELRVVRSSHDGVDSYAVAGFDVSAWHATEERLIQ
                     ALHLDRLTNLANQPALIPALLEAQQQADAHGTPAALLLLDLDDYQRVNRALGYDAG"
     gene            946..2283
                     /locus_tag="VHV99_29955"
     CDS             946..2283
                     /locus_tag="VHV99_29955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX3639632.1"
                     /translation="MFERHFVRAIPRRVEVLQQCAEDADPASGAQFVYLGRQPILDRD
                     GALNAYELLFRAGAHNYAEVSDDARATAQVVARALGGIGVAAALGRHRGFVNIDRALL
                     FNDIVHLMPPERFVLEILETVRFDAQLGRRLTELRRAGFQVALDDVCELSDELLAALP
                     HADIVKIDFLQVERSALARLASVVRGHGKTLIAEKIETREDFALARELGFDLFQGYFF
                     ARPQVLAAPRNRSPRPGLLRLLALLSRDAGIVELEAELKLNPSVVMQLLRLVNSSAFG
                     LGRNIASLREAIIATGTRQIARWAQLLLYADSADLPWRADPLVQLAGTRSRFMELAAS
                     WLRPADGEFADAAFMTGIFSLVHVVVGSTPAAVLEKLGLAPQIREAIVTGCGPLGALL
                     RIAEAAGEGADAALIAAGSDVPAGFAALTPEVLAELNLSAAAWFGAHIQEAIV"
     gene            2588..3151
                     /locus_tag="VHV99_29960"
     CDS             2588..3151
                     /locus_tag="VHV99_29960"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX3639633.1"
                     /translation="MKRKLVPVALAAVLSVGFAMHSLTASATLKPGDAAPPFTTEASL
                     GGKTYMYSLSDELKKGPVVLYFYPAAFTKGCTIEAHEFAEAVDEYKKYGATVIGVSHD
                     NIDTLTKFSVSECRSKFPVAADADSKVIGAYDAGLPMHSSMANRVSYVIAPDGKIIYE
                     YTSLSPEKHVENTLKAVKDWAATHKSP"
     gene            3339..3803
                     /locus_tag="VHV99_29965"
     CDS             3339..3803
                     /locus_tag="VHV99_29965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639634.1"
                     /translation="MPILVALIALIALVYGAVRAFYALQAAFGLVVAVGVAVLVALLL
                     IGAVVYWWRRRKEVAANIRDGDWTHELKGTWGSVRLAAGKRLCEVRLGGERGDYIFAD
                     LLGAEVQGHDAQWQVALKVKDAKRQVWVLPMRGERQAKQWVRVFLLAVEQRL"
BASE COUNT          714 a         1291 c         1542 g          918 t
ORIGIN      
        1 ccggcatcgt agccgagcgc gcggttcacg cgctgatagt cgtcgaggtc gagcagcagc
       61 agcgcagccg gggtgccgtg ggcgtcggcc tgctgttgcg cctcgagcag cgcggggatc
      121 agcgccggct gattggccag attcgtcagg cggtccagat gcagcgcctg gatcaggcgt
      181 tcctcggtgg cgtgccaggc cgatacgtcg aagccggcca ccgcatagct gtcgacgccg
      241 tcgtggctgc tgcgcaccac gcgcaactcg acggtgatcg ggtaggtcag ggacttgatg
      301 aggccgagcg tggccttttc gacgttgccc gtggcggtgg cgcgcttcag cagcgcgtcg
      361 aggcgcggca cgtcggcggg cgccacgaga tcgtgcagcg tgatggtctc gagatactcc
      421 cggtgatagc cgatgaagcg caggctggcg tcggatacat agaggaagcg cagcgtctga
      481 tcgacgagcg ccagcagatc gacggcgccg accacctgtt ccagttgcgc agtgcggctt
      541 gcgcctgact gcggtttgct gggggcgcgc cggccgaacg cacgaaaccg gtcgatgacc
      601 gtgcgtaagg ggccccggcg gggcgcgatg atcctgttcg cttccatgtt tgtcgctggc
      661 aacctgtgtt cgtctgcagg cgggggcggc tccctgcccg ccatgtccgg gcggttaaga
      721 ggtatcaggc cgccccatca gaacaagata acgaccggcg gcgcgaaatc tttagcgcct
      781 gcggtaaaaa acaaatggtg aagaaagggc ggcgtggtcc ggggcgcggc gcggccaatt
      841 cagttaaagt gcgacggcct ggcatccgtt aatacgtcaa acccgttgtg cagtgtcggc
      901 caggacgtcg ctgcgtagct ttccgaacac ttgcaccgtt gatccatgtt tgaacgtcac
      961 tttgtcaggg ccattccccg gcgcgtcgag gtgctccagc aatgtgccga agacgccgac
     1021 ccggcgtccg gggcgcagtt cgtctatctg ggccgccagc cgattctcga tcgcgacggc
     1081 gcgctcaatg cttacgaatt gctgtttcga gccggcgcgc ataactatgc cgaggtcagc
     1141 gacgacgctc gggcgaccgc ccaggtggtg gcgcgcgcgc tcggcgggat cggcgtggcg
     1201 gccgcgctgg gccgacaccg cggcttcgtc aatatcgacc gcgcgctgct ctttaacgac
     1261 atcgtccatc tgatgccacc cgagcgcttc gtgctggaga tcctcgaaac cgtcaggttc
     1321 gatgcgcagc tgggccgccg gctgaccgag ttgcgccgcg ccggcttcca ggtggcgctc
     1381 gacgacgtgt gcgaactgtc ggacgagctg cttgccgcgc tgccgcatgc cgacatcgtc
     1441 aagatcgact tcctgcaggt ggagcggtcc gcgcttgcca gattggcgtc ggtggtacgc
     1501 ggccatggca agacgctgat cgcggagaag atcgagacgc gcgaagactt tgcgctggca
     1561 cgtgaactcg gcttcgatct gttccaggga tacttcttcg cacggccgca ggtgctggcc
     1621 gcgccgcgca accgctcgcc gcgccctggc ctgctgcgtt tgctggcgct gctgtcgcgc
     1681 gacgccggga ttgtcgagct cgaggcggag ctcaagctga acccgagcgt ggtgatgcaa
     1741 ctgctgcgcc tcgtcaattc gagcgcgttc ggactggggc gcaatatcgc ttcgctgcgt
     1801 gaagccatca tcgccaccgg cacgcggcag atcgcgcgct gggcgcagct gctgctgtac
     1861 gccgacagtg ccgatctacc gtggcgagcc gatccgctgg tgcaactggc cggcacgcgc
     1921 tcgcgcttca tggaattggc cgcgagctgg ctgcgcccgg ccgacggcga attcgccgat
     1981 gccgccttca tgaccggcat tttttctctg gtccacgtcg tggtcggcag tacgcccgcg
     2041 gccgtcctcg aaaaactcgg ccttgcgccg cagattcgcg aggcaatcgt cacgggctgc
     2101 ggcccgcttg gcgccttgtt gcggattgcc gaagcagccg gcgagggtgc cgatgccgcg
     2161 ctgatcgcgg cgggatccga tgtcccggcc ggtttcgccg cgctcacgcc cgaggtgctg
     2221 gccgaactga acctgtcggc cgcggcgtgg tttggcgccc atatccagga agcgatcgtc
     2281 tgacgggagg ggcgccgcga tgccgcccgg gcttctcccg cgcgttgcgt ggtttcgacc
     2341 ggatgggttg gttctggcag cggatcagac tggttcgctg gctggcaagc ctgctccggg
     2401 tcgccgcccg gtcgttgtat cgcgccgctg cgggcgcggg tgcccggatg cgtcctttct
     2461 tcaggttgcg acgtattttg tcggtacgtg gtccaatagc aggtggagat gccgggggtc
     2521 gactggacgc cgcgaccgtc ggctaattgt gctaaccaca tgcacggatg tttcgagggg
     2581 actgcaaatg aagagaaagc ttgtaccggt ggcgctggct gccgtgttat cggtgggatt
     2641 cgcgatgcat tcgctaacgg cctcggccac gctcaagccg ggcgatgccg cgccgccgtt
     2701 caccacggag gcttcgctgg gcggcaagac ctacatgtat tcgctttccg acgagctgaa
     2761 gaaagggccg gtggtgctgt atttctaccc ggcggcgttc acgaagggtt gcacgatcga
     2821 agctcacgag tttgccgaag ccgtcgacga gtacaaaaaa tatggcgcga ctgtgatcgg
     2881 cgtatcgcac gacaacatcg acaccttgac gaagttttcg gtgagcgaat gccgcagcaa
     2941 attcccggtg gcggccgatg ccgactcgaa ggtgatcggc gcatacgatg ccggcttgcc
     3001 gatgcatagc tcgatggcca atcgcgtgtc gtacgtgatc gctccggacg gcaagatcat
     3061 ttacgagtac acgagtcttt cgccggagaa gcacgtcgag aacacgctga aggcggtgaa
     3121 ggactgggcg gcgacgcata agtcgccgta ggaccgggca gtgacctata agcagccgta
     3181 ggactgggcg gtgacgcata agtagccgta gggccttggc attcccgacg cgctcatggc
     3241 gcttatcacg cgaggctgcg catttttcgc aatggttttt tgccgtgaga tttggggctg
     3301 cgccgtgcta ggctgatttt ttgtctagcg agcttgcgat gccgattctt gttgcgttga
     3361 ttgcgctgat cgcgctggtg tatggcgccg tgcgggcgtt ttatgccttg caggcggctt
     3421 ttgggttggt cgttgcggtt ggggtggctg tactggttgc tttgcttttg attggggcgg
     3481 ttgtttattg gtggcggcgt cggaaggaag tcgctgccaa tattcgcgat ggcgattgga
     3541 ctcatgagct caaggggacc tgggggtcgg tccggcttgc tgcaggcaag cgcttatgtg
     3601 aggtgcgcct tggcggcgag cggggggatt atatttttgc cgatttgctg ggggctgagg
     3661 tgcagggcca tgatgctcaa tggcaggtcg ctttgaaggt taaggacgct aagcggcagg
     3721 tttgggtttt gcccatgcgg ggcgagcggc aggcgaagca gtgggtgcgg gtttttttgc
     3781 ttgctgttga gcagaggctt tagaagtggg ggtttttgtt tgcctccggc ggtggggggc
     3841 ttgggttttt gcctgctcgg cgcttggttg tatgcctgcg gcgttggcct tttcctgttt
     3901 tgtttgtctg ctcgcgcgtg gttgtctgta tgcctacggg gttggccttt ccttgaattg
     3961 atattggtct attagcgtcg cccctgtgcg gggcaggcac ctacttttct ttgcctgccg
     4021 caaagaaaag taggcaaaag aaagcggctc accccgccaa ctcttaagcg ggtcccccgc
     4081 acagtcgcgg tagtggcgca tctggaatcg gtgccctcgc acattcagcc ctggtgacaa
     4141 ggctgtcata cttcccgcct cgcactgcgc gctcgccgga agggttcatc atgcaaacca
     4201 gcggcttcgt ttgggagcag gtgggagcca tcggctttgc ctcggcgaca caccccgacg
     4261 agacggcgag aaggaggcga agaggcaata cacgccgcac catggcgtca aacgaaccgt
     4321 gagagcaaaa acgcagcggg cggctttaag cagtgccctt cggcgcgcgc agcgccgccg
     4381 gaagcatgac ggccttgtca ctcacgccga atgtgcgaga acaccgattc cagatgcacc
     4441 actaccgtga ctgtgcggcg gaccc
//
LOCUS       DATASH010001129         2469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_4943540,
            whole genome shotgun sequence.
ACCESSION   DATASH010001129 DATASH010000000
VERSION     DATASH010001129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4943540"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VHV99_29970"
     CDS             complement(<1..324)
                     /locus_tag="VHV99_29970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEX3639635.1"
                     /translation="MSTHDTHDTPVPNGPLDLLIADATLIFADGSAHAAQLGIRGAHI
                     AYIGDARPAATRTLSLPGRVVTPGFVNAHYHAGLNFVRGVAPDCGFAPSYTPGLPQAS
                     WLDAEE"
     gene            complement(321..1124)
                     /locus_tag="VHV99_29975"
     CDS             complement(321..1124)
                     /locus_tag="VHV99_29975"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3639636.1"
                     /translation="MTLPSPSPAASVNMAGKVCIVTGAASGIGRATARQLAVNGATVV
                     IADVTMDVIEGGVPTAALIARDGGHAHFVATDVSDTAQVDALVAHTVERFGRLDVLVN
                     NACVRHARPLLELEDAHWQRLLDVNLTGVFRCCRAAVRQMLQQAPEGEVRGRIVNLSS
                     QHGMIAAPGDLGYGTTKAAIDYMTRQIATDYAPHGIVCNAVAPGKIRTGAGGRAVDPA
                     VLERANRRTPWPRLGQPDDVARAVLFLCSDLSTFTTGTTLMVDGGWMAA"
     gene            complement(1156..2133)
                     /locus_tag="VHV99_29980"
     CDS             complement(1156..2133)
                     /locus_tag="VHV99_29980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3639637.1"
                     /translation="MTTFITGSSGFVGLALVERLLAGGETVIGFDRSAPRAQALQAFA
                     ALPGRFVALTGDVRDGDALREAMRTHRPSRLVTLAAITADAQRERHMPQTIFEVNVGG
                     VLAAMTAAADAGVERILHGSSGSAYGLSGRAPHPLVEDVTPLLPEGLYGISKRAAEDA
                     ALRLSALHGVPLVIGRLGTCFGPWEADSGVRDTLSAPLQVVTKARRGETAVLPRAGRR
                     DWLYVRDAASALLALLEAPHWPHSVYNLAAGFEWSVADLCERLARRYPAFTWRIAQHE
                     EAPNIDYFADYDRASMDNTRLVADTGFTPRFDLAEAEADFHRWITTLGA"
     gene            complement(2135..>2469)
                     /locus_tag="VHV99_29985"
     CDS             complement(2135..>2469)
                     /locus_tag="VHV99_29985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3639638.1"
                     /translation="IARALLMNPEVILMDEPFGALDEQTKMDMHAELIRIWRESRSTI
                     VFVTHGIDESLALGTHVAVMSARPGRIRELLPIELDRPRDPTSAEFNDYKRHILSLLR
                     PEAVMEPA"
BASE COUNT          403 a          823 c          861 g          382 t
ORIGIN      
        1 ttcttcagcg tcgagccacg aggcttgcgg caaccccggc gtataggacg gcgcgaagcc
       61 gcaatccggc gccacgccac gcacaaagtt caggcccgcg tgatagtgcg cgttgacgaa
      121 tcccggcgtc acaacgcgtc ccggcagcga cagcgtgcgc gtcgccgcgg gccgcgcgtc
      181 gccgatatag gcgatgtgtg cgccacgaat gccgagttgc gccgcgtggg ccgagccgtc
      241 ggcgaaaatc agcgtggcat ccgcgatcag cagatcgagc gggccgttcg ggacgggcgt
      301 gtcgtgcgta tcgtgcgtgc tcatgccgcc atccagccgc cgtccaccat cagcgtggtg
      361 ccggtcgtga aagtcgagag gtcgctgcac aggaacagca ccgcgcgcgc gacgtcgtcc
      421 ggctgtccga gacgcggcca gggcgtgcgc cggttggcgc gttcgagcac ggccggatcg
      481 accgcacggc cgccggcgcc ggtccggatc ttgcccggcg cgaccgcgtt gcagacgatg
      541 ccgtgcggcg cgtaatcggt ggcgatctgg cgcgtcatat agtcgatcgc ggccttggtc
      601 gtgccgtagc cgaggtcgcc tggcgcggcg atcatgccgt gctgtgacga cagattgacg
      661 atccgcccgc gcacttcgcc ttcaggcgct tgctgaagca tctgccggac ggccgcgcgg
      721 cagcagcgga atacgcctgt cagattgacg tcgagaagac gttgccagtg tgcgtcttcg
      781 agttccagca gcggccgtgc atgacgaaca caggcgttgt tgaccagcac gtcgagccgc
      841 ccgaagcgct cgaccgtgtg cgccaccagc gcatcgacct gtgccgtgtc ggatacgtcg
      901 gtcgcgacga aatgggcgtg gccgccatcg cgcgcgatca gtgcggcggt cggcacgccg
      961 ccttcgatca cgtccatcgt gacgtcggcg atcacgaccg tcgcgccgtt gacggcgagc
     1021 tggcgcgcag tcgcccggcc gatgcccgac gcggcgccag tgacgatgca caccttgccg
     1081 gccatgttca cgcttgcggc cggggagggc gagggtaggg tcatgacgtt actcgtacaa
     1141 tcgcggggca ggcgttcacg cgcccagcgt ggttatccag cgatggaagt cggcttccgc
     1201 ttcggccaga tcgaaccgtg gcgtaaagcc ggtatcggcg accagccgcg tgttgtccat
     1261 cgacgcgcgg tcgtaatcgg cgaagtaatc gatattcggc gcctcctcgt gctgcgcaat
     1321 gcgccacgtg aacgcgggat aacgccgcgc gagccgttcg cacaggtcgg cgacgctcca
     1381 ctcgaagccc gcagcgaggt tgtagacgga gtgaggccag tgcggcgctt cgagcaaggc
     1441 aagcagcgcc gaggccgcat cgcgcacgta gagccagtcg cggcggccgg cgcgtggcag
     1501 cacggcggtt tcgccccggc gcgctttcgt gaccacctgc agcggcgcgc tcaaggtgtc
     1561 gcgcacgccc gagtcggctt cccacgggcc gaagcaggtg ccgagccgtc cgatcacgag
     1621 cggcacgccg tgcaacgcgg aaagacgcaa tgcagcgtct tcggcggcgc gcttggagat
     1681 gccgtagagg ccttcgggca gtagcggcgt gacgtcttcg acgagcgggt gcggcgcgcg
     1741 accgctcagt ccgtacgccg agccggaact gccatgaaga atgcgttcga cacccgcatc
     1801 ggcggccgcg gtcatggcgg cgagcacacc gccgacgttc acctcgaaaa tggtctgcgg
     1861 catgtgccgc tcgcgctgcg catccgcggt gatcgcggcg agcgtgacga gtcgcgacgg
     1921 ccgatgcgtg cgcatggcct cgcgcaacgc gtcgccatcg cgcacgtcgc cggtcagcgc
     1981 aacgaagcgg cccggcagcg cggcaaatgc ctgcaacgct tgcgcgcgcg gcgcgctacg
     2041 gtcgaagccg atgaccgtct cgccgccggc gagcaggcgc tcgacaagcg cgagaccgac
     2101 gaaaccgctg ctgcccgtga tgaacgtggt catctcaggc aggctccatc accgcttccg
     2161 gccgcagcag cgagaggata tggcgcttgt agtcgttgaa ttcggcgctg gtcggatcgc
     2221 gcggccggtc gagttcgatc ggcaacagtt cgcgaatgcg gcccggacgc gccgacatca
     2281 ccgcgacatg cgtgccgagc gcaagcgatt cgtcgatccc gtgcgtgacg aacacgatcg
     2341 tgctgcgact ctcgcgccag atgcggatca gttcggcgtg catgtccatc ttggtctgct
     2401 cgtcgagcgc gccgaacggc tcgtccatca gaatcacttc cgggttcatc aacagcgcac
     2461 gcgcaatgc
//
LOCUS       DATASH010001130         1628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_197352,
            whole genome shotgun sequence.
ACCESSION   DATASH010001130 DATASH010000000
VERSION     DATASH010001130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197352"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..1311
                     /locus_tag="VHV99_29990"
     CDS             172..1311
                     /locus_tag="VHV99_29990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3639639.1"
                     /translation="MATRRGALVRRRLARDENNPIEVLSEIKYLLDNRLRLGKVGGPF
                     MPRPGALRALIALSSLTIAVLGGAVAADDPFDIPLWAYPGSKPDSTVADSAELHRLPG
                     SAARFTLRQVLNRFDPPDWHPQNHPPMPDVVAHGRAPKLYACAYCHLPNGTGRPENAT
                     LAGLPEAYIVAQLGAFRSSARRSPLPSYVPGLSMHTVAVNATDADVASAARYFSRLKA
                     EPRVKVVETARIPRMQEAAFVYRRVPGGGTEPLGTRIIEGPVDFERHELRDDALTYVA
                     YVPPGSIKRGRSIAKTGANGLTTACVTCHGAKLTGVDSIPRLAGHFPTYVMRQLVAFK
                     TRARSTPASQPMQGVVARMSVADMIAVAAYAGSLNSATSARFRNR"
     gene            complement(1350..>1628)
                     /locus_tag="VHV99_29995"
     CDS             complement(1350..>1628)
                     /locus_tag="VHV99_29995"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX3639640.1"
                     /translation="RYFLLTGQKLSAREAKEQGAVNEIVPREMLLPRAWELARQMTKQ
                     PPLTLRYTRMAFSTRFRRRLQEGLSYGLALEGISAFEVARLNAQKTKA"
BASE COUNT          314 a          505 c          489 g          320 t
ORIGIN      
        1 gcgaggcgac tggcggagca ccgaaaggct ttggtcgaag ctgtgcgacg tattacagat
       61 cccaaccaag gcacaacgcg atagcggagc catagcacgt tctctcggcg ctcgacgtcc
      121 atgagggcca gtcgtcgcgg cgtgaaattg gtcgtgaaaa cgtcgagatg cttggcgacg
      181 cgtcgcggcg cgctagtccg gcgccgtctt gccagagatg aaaacaatcc catcgaagtc
      241 ctgagcgaga tcaaatacct actcgacaac cggctcaggt tgggcaaagt tggtggaccc
      301 tttatgcccc ggcctggcgc cctgcgtgcg ctcatcgcgc tatcgtcgct gacgatcgct
      361 gtcctcggcg gcgccgtcgc cgcggatgat cctttcgaca taccgctatg ggcgtatcct
      421 ggcagtaaac ctgattccac tgtagccgac agcgcggaac tccatcgtct tccgggcagt
      481 gccgcgcgtt ttaccctgcg gcaggtcttg aatcggttcg atccgccgga ctggcatccg
      541 caaaatcatc cgccgatgcc ggacgtcgtt gcccacggtc gagcaccgaa actttatgcg
      601 tgtgcgtatt gtcatcttcc caacggaacc ggccggccgg agaacgcgac tctcgccggt
      661 ttgccggaag cctacatcgt cgcgcagctg ggagcctttc gctcttccgc gcgtcgcagt
      721 cccttaccca gctatgtgcc gggactcagc atgcacacgg tagcggtgaa tgcaacagat
      781 gccgacgtcg cgtcggcggc gcgctacttc tcgcgactga aggccgagcc gagggtgaag
      841 gtggtcgaga ccgcgcgcat tccacggatg caggaagccg cgttcgtcta caggcgcgtt
      901 ccaggaggcg gcaccgaacc gcttggcaca cgcatcatcg aaggcccagt cgatttcgag
      961 cgacacgagc tgcgcgacga cgcactgact tacgtggcct acgtgccgcc gggcagcatc
     1021 aagcgcggac gctcgatcgc gaaaaccggc gcgaacggac tgaccactgc ctgcgtcaca
     1081 tgtcatggcg ccaaactcac cggtgttgat tctattcctc ggctcgccgg ccactttccg
     1141 acgtacgtca tgcgacaact cgtggcgttc aagacccgcg cgcggtcaac accggcatcg
     1201 caaccaatgc agggcgtggt agcgcgcatg tctgtagcgg atatgatcgc ggttgccgct
     1261 tacgcgggtt cgctcaattc agcgacttct gctcgattca gaaatcggta gttgtccgtg
     1321 cggccctgct gttgccacaa aacgaaaagc tacgcttttg tcttttgagc gttcagccgt
     1381 gcaacttcga aggcagaaat gccttccagc gcgagtccgt aggacaatcc ttcctggagg
     1441 cggcggcgaa agcgagtcga gaaagccatc ctcgtgtatc gcagggtgag cgggggctgc
     1501 ttggtcattt ggcgagccaa ttcccaggcg cgtggaagca acatctcgcg cggaacgatc
     1561 tcgttgactg caccctgctc cttcgcttct ctcgcactca gtttctgacc ggtgaggaga
     1621 aaatagcg
//
LOCUS       DATASH010001131         2247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_5933177,
            whole genome shotgun sequence.
ACCESSION   DATASH010001131 DATASH010000000
VERSION     DATASH010001131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5933177"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VHV99_30000"
     CDS             complement(<1..462)
                     /locus_tag="VHV99_30000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006761975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX3639641.1"
                     /translation="MKRRIFLTLAAAAAGVLFNAPVAQAADPVKIGFLVKQPEEPWFQ
                     DEWKFAEIAAKEKGFTLVKIGAPSGEKVMSAIDNLSAQKAQGFVICTPDVKLGPGIVA
                     KAKADGLKMMTVDDRLVDGAGKPIASVPHMGISAYNIGKQVGDGLAAEIKKR"
     gene            complement(742..1554)
                     /locus_tag="VHV99_30005"
     CDS             complement(742..1554)
                     /locus_tag="VHV99_30005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004527760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3639642.1"
                     /translation="MKRLADKVALITGAGRGIGAAIALAFAREGAAVVLAELDIETAE
                     RTAREIRAAISASDTGEPNGASEAKVLAVQTDVTHSASVQHAVSEAEHAFGALDVLVN
                     NAGINVFCDPLTMTDDDWRRCFAVDLDGVWNGCRAVLPGMVERGAGSIVNIASTHAFK
                     IIPGCFPYPVAKHGVIGLTRALGIEYAPRNVRVNAIAPGYIETQLTHDWWNEQADPVA
                     AKQATLDLQPMKRIGRPEEVAMTAVFLASDEAPFINASCITVDGGRSAVYHD"
     gene            complement(1551..2231)
                     /locus_tag="VHV99_30010"
     CDS             complement(1551..2231)
                     /locus_tag="VHV99_30010"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008674 -
                     2-dehydro-3-deoxy-6-phosphogalactonate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0034194 - D-galactonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HEX3639643.1"
                     /translation="MQPHINLPAPYMPHAGLIAAFDVCPLIAIMRGVTPADAAEHGQA
                     LYEAGFRIIEVPLNSPQPFDSIAAIRKALPADAIVGAGTVLHPSFVNDVKSAGGELIV
                     MPHSDPDVVGAAKAQGMACAPGVATPNEAFIALKNGADVLKMFPAEQLGCQVVKAWRA
                     VIAAQVPLVPVGGITPDNMGPFLSAGANGFGLGSALYKPGQSVAVTGSHAKAFINGLR
                     IARAGAKK"
BASE COUNT          349 a          767 c          657 g          474 t
ORIGIN      
        1 gcgcttcttg atttccgcgg ccaggccgtc gcccacctgc ttgccgatgt tgtacgccga
       61 gatgcccata tgcggcaccg aagcgatcgg cttgcccgcg ccgtcgacga ggcggtcgtc
      121 caccgtcatc atcttcaggc catcggcctt cgctttggcg acgatgcccg gtccgagctt
      181 gacgtcaggc gtgcagatca caaaaccttg cgctttctga gccgacagat tgtcgatagc
      241 gctcatcacc ttctcgcccg acggcgcgcc gattttcacc agcgtgaagc ccttctcctt
      301 ggcggcaatt tccgcgaact tccattcgtc ctggaaccac ggttcttccg gctgcttcac
      361 gaggaagccg atcttgaccg gatccgcggc ttgcgcaacc ggtgcgttga agagcacacc
      421 cgccgccgct gctgccagcg tgaggaaaat tcttcgtttc atgatgcgtg tctcctgact
      481 aattgataac gagaaggtat cggccgcgtc ttcttgtacc ggttgtgaca cacgcggcca
      541 gcgcgtcgtc cggtgaaacc gttgcttact gttgctgccg tttgttcttg gcgcccgttc
      601 tttgccacgt tctttgcccc gttcttgttg cccgttcctg ttgcccgttc ctgttgcccg
      661 ttcctgttgc ccgttcctgt tgctcgttcc tgttgctagc actgccggtc ccgcatcctg
      721 ttgcgcgccg ctgcgccatg ttcagtcgtg ataaaccgcc gagcggccgc catccacggt
      781 gatgcaactc gcgttgatga acggcgcttc atccgaggcg agaaacaccg ccgtcatcgc
      841 gacttcttcc gggcggccga tacgtttcat cggttgcaga tcgagcgtcg cctgtttcgc
      901 ggccacggga tcggcttgtt cgttccacca gtcgtgcgtc agttgcgttt cgatgtaccc
      961 tggcgcgatc gcattgaccc gcacgttgcg cggcgcgtat tcgatgccga gcgcacgcgt
     1021 caagccgatc acgccgtgct tggcgaccgg gtaaggaaaa catcccggaa tgatcttgaa
     1081 cgcatgcgtc gacgcgatat tcacgatgct gcccgcgcca cgttcgacca tgcccggcag
     1141 caccgcgcgg catccattcc agacgccgtc gagatcgacc gcgaagcaac ggcgccagtc
     1201 gtcgtcggtc attgtcagcg ggtcgcagaa cacgttgatg ccggcattgt tgaccagcac
     1261 gtcgagcgca ccgaacgcgt gctccgcttc gctcaccgcg tgctggactg acgcggagtg
     1321 cgtcacgtcg gtctgcaccg cgagcacttt tgcttcgcta gctccgttcg gttcacctgt
     1381 gtcacttgca ctgatcgccg cacggatttc gcgcgcggtc cgctcggccg tttcgatatc
     1441 caactccgcc aggacgaccg cagcgccctc gcgcgcgaac gccagcgcga ttgccgcacc
     1501 gatgccgcgc ccagcgccgg tgatcaacgc gactttgtcg gcgagccgct tcatttcttc
     1561 gcgcctgccc gggcgatgcg cagcccgttg atgaacgcct tcgcgtgcga gccggtcacc
     1621 gccacgctct gacccggctt gtacagcgcc gaaccgaggc cgaaaccatt cgcgccggcg
     1681 ctcaaaaacg gacccatgtt gtccggcgtg atcccaccga ccggcaccag cggcacttgc
     1741 gccgcgatca ccgcgcgcca cgccttcacg acctggcagc cgagttgttc ggcggggaac
     1801 attttcagca cgtccgcgcc gtttttcagc gcgatgaagg cttcattcgg tgtcgccacg
     1861 cccggcgcgc aggccatgcc ttgtgctttc gcggcgccga cgacgtccgg atcgctatgc
     1921 ggcatcacga tcagctcgcc gcccgcggac ttcacgtcgt tgacgaaact gggatgcagc
     1981 accgtgcccg caccgacgat cgcatccgcc ggcaacgcct tgcggatcgc cgcgatgctg
     2041 tcgaacggct gcggcgaatt caacggcact tcgataatgc gaaagcccgc ttcgtacagg
     2101 gcctggccgt gctcggcggc atcagccggc gtcacgccgc gcatgatcgc gattagcgga
     2161 cacacgtcga acgcggcgat caaacccgcg tgcggcatgt acggggcggg cagattgatg
     2221 tgaggttgca tgtcggttcc ttgtttc
//
LOCUS       DATASH010001132         2501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_593131,
            whole genome shotgun sequence.
ACCESSION   DATASH010001132 DATASH010000000
VERSION     DATASH010001132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593131"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="VHV99_30015"
     CDS             complement(<1..784)
                     /locus_tag="VHV99_30015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HEX3639644.1"
                     /translation="MTTAKPDLLSLATRFALLPDAQRKAFLTKLDAAGIDFRVLPIPP
                     RAERTASVPASFAQTRLWLHARMIDEPAAYHITERLRLDGALDANALRLSCDALIARH
                     EALRTTFAESADGVLQTVHAPSRCPWHASDVSTLPVHERETRAAAIAQADEAQAFDLL
                     LGPLMRAHLIRLDATTHWLALTTHHIVSDGWSADVLLAELASFYHAYAKNEAVSLTPL
                     PVQYADYALWQRRWLDAGERERQLAFWRGRLDASRDVLMLPGA"
     gene            complement(882..>2501)
                     /locus_tag="VHV99_30020"
     CDS             complement(882..>2501)
                     /locus_tag="VHV99_30020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006499075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEX3639645.1"
                     /translation="KAGAAYLPIDPDYPADRIAYLLTDANPAVVLTQPHLRDRVEAAV
                     ETIDVKILTVDELLDSGAALTAPVEVAPEQLAYLIYTSGSTGKPKGAGNTHRALANRI
                     AWMQDAYRLSAEDVVLHKTPFGFDVSVWEFVWPLAIGAKLAIAAPGDHRDPARLVAAI
                     ETHRVTTLHFVPSMLAAFVAHLEDFKAAARCASIERIVASGEALAPELVARVARHLPN
                     ARLYNLYGPTEATIDVSHWRCDARDRNAGSVPIGHPIANLQLHVLDAALQPLPSGAIG
                     ELHLGGVGLARGYLGRAALTAERFVPDPFVEGARLYRTGDLARRRADGALDYLGRIDT
                     QVKLRGQRIEPGEIEALLRAEQGVHDAVVMVRDEQLIGYIVRGVGNTIDTNVLLDALR
                     AQLPAYMVPSQLIELDVLPVTPNGKCDRHALPAPARGASSGKSAAASLQTDTERELAG
                     IWKRVLRLEAVGRDDDFFALGGHSLLATQANAQANLHWALILPLRTLFDERTLQRCAA
                     AIDRALAERDVRAGGGTASAIDALLGEFELQ"
BASE COUNT          456 a          802 c          815 g          428 t
ORIGIN      
        1 cggcgcctgg caacatcagc acgtcacggc tcgcatccag ccggccacgc cagaatgcca
       61 actgacgctc acgctcgccc gcgtcgagcc aacggcgctg ccacagcgcg taatccgcgt
      121 actggaccgg cagcggtgtc agcgacactg cttcgttctt cgcatacgcg tgatagaacg
      181 aggccaactc cgcaagcagg acgtccgcgg accagccgtc ggacacgatg tgatgcgtcg
      241 tcaacgcgag ccagtgagtc gtcgcgtcga gccggatcaa atgcgcgcgc atcagcggac
      301 cgagcaacag atcaaaagcc tgcgcttcat ccgcctgtgc aatggcggcc gcgcgcgtct
      361 cgcgctcatg cacgggcaag gtgctcacgt cgctcgcatg ccacggacat cgtgacggcg
      421 catgcacggt ctgcaacacg ccgtccgcgc tctccgcgaa cgtcgtgcgc aacgcttcat
      481 gccgcgcgat caacgcgtcg cacgaaagac gcagggcatt cgcatcgagc gcaccgtcca
      541 gacgcagacg ctcggtgatg tgatacgccg cgggctcgtc gatcatgcgc gcgtgcagcc
      601 acagacgcgt ttgcgcgaac gaagccggca cgctggcggt ccgctcggcg cgcggcggaa
      661 tcggcaacac gcgaaaatcg atgccggctg cgtcgagctt cgtgagaaat gctttgcgct
      721 gcgcgtccgg caaaagcgcg aagcgcgttg cgagggaaag caggtccggt ttggctgtcg
      781 tcatgcgagg ttgtctcagt gctcagtgct ggtcggtgta gtggcgtgat ggcgtaagga
      841 cgtagtggcg gaatggcgga atggcccgat ggcttgctcg cttattgcag ttcgaattca
      901 ccgaggagtg catcgatcgc gctcgcggtg ccgcctccgg cacgcacgtc ccgctctgcc
      961 agcgcgcgat cgatcgcggc ggcacagcgt tgcagcgtgc gttcgtcgaa tagcgtgcgc
     1021 agcggcagga tcagcgccca atgcagattg gcctgcgcgt tggcctgcgt cgcgagcaac
     1081 gagtgaccgc ccagcgcgaa gaagtcgtca tcgcggccaa ccgcctccag acgcagcaca
     1141 cgcttccaga ttccggcgag ttctcgctcg gtatcggttt gcagcgatgc ggcggcgctc
     1201 ttgccggaag aggcaccacg cgccggagcc ggcagcgcat ggcggtcaca cttgccgttc
     1261 ggcgtgacag gcagcacatc caactcgatc agttgcgaag gcaccatgta cgcgggcaat
     1321 tgggcacgca gcgcatcgag cagaacattc gtgtcgatcg tgttgcccac gcctcgcacg
     1381 atatagccga tcaattgctc atcacgcacc attaccaccg cgtcgtgtac accctgttcc
     1441 gcacgcaaca aggcttcgat ttcaccgggt tcgatgcgct gaccccgcag cttgacttgc
     1501 gtatcgatac ggccgagata atcgagcgca ccgtccgcgc gacggcgcgc gaggtcgccg
     1561 gttcgataga gacgtgcgcc ttcaacgaag ggatcgggaa cgaagcgctc cgccgtcagc
     1621 gcggcacggc cgagatagcc gcgcgcgagt cccacgccgc cgaggtggag ttcgccgatt
     1681 gcccctgagg gcagcggctg taacgctgca tcgagcacat gcagttgcag attcgcgatt
     1741 ggatgaccga tcggcacgga ccctgcgttt ctatcccgtg catcgcaacg ccagtgcgag
     1801 acgtcgatgg tggcttcggt cgggccatac aggttataga ggcgcgcatt cggcaaatgc
     1861 cgcgcgacgc gcgccaccag ttccggcgcg agcgcttcgc cgcttgccac gatccgctcg
     1921 atactcgcgc agcgggccgc cgccttgaaa tcttccagat gcgcaacgaa cgcggcgagc
     1981 atcgacggta cgaaatgcaa cgtggtgacg cgatgcgttt cgattgccgc gaccagacgt
     2041 gcgggatcgc ggtgatcgcc cggtgccgca atggcgagtt tcgcgccgat ggcgagcggc
     2101 cagacaaact cccataccga cacgtcgaag ccgaacggcg tcttgtgcag cacgacgtct
     2161 tcggcgctca gccgatacgc gtcctgcatc cacgcgatcc ggttcgcgag cgcgcgatgt
     2221 gtattgcccg cgcccttcgg tttgccggtc gaacccgatg tgtagatcag gtaagcgagt
     2281 tgttcgggcg cgacctctac cggtgcggtg agcgcggcgc cggagtcgag tagttcgtcg
     2341 acggtcagga tcttgacgtc gatggtttcg accgccgcct cgacacggtc gcgcagatgc
     2401 ggctgcgtga gtacgaccgc cgggttcgcg tcggtgagga gatacgcgat tcggtcggcc
     2461 ggataatcgg gatcgatagg cagataagct gcgcccgctt t
//
LOCUS       DATASH010001133         5366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Paraburkholderia sp. isolate SMAG_U5515 k141_6527000,
            whole genome shotgun sequence.
ACCESSION   DATASH010001133 DATASH010000000
VERSION     DATASH010001133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956339
            Sequence Read Archive: SRR11836029
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paraburkholderia sp. (soil metagenome)
  ORGANISM  Paraburkholderia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Paraburkholderia.
REFERENCE   1  (bases 1 to 5366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:41:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,959
            Genes (coding)                    :: 5,765
            CDSs (with protein)               :: 5,765
            Genes (RNA)                       :: 49
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 64 of 194
            Pseudo Genes (incomplete)         :: 142 of 194
            Pseudo Genes (internal stop)      :: 36 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5366
                     /organism="Paraburkholderia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6527000"
                     /isolate="SMAG_U5515"
                     /isolation_source="soil"
                     /db_xref="taxon:1926495"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VHV99_30025"
     CDS             <1..582
                     /locus_tag="VHV99_30025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008354015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme GlgX"
                     /protein_id="HEX3639646.1"
                     /translation="PAGRAMTDFVARVIALRKNYPLLREMRFLHGDREVLPGLYDVSW
                     FDESGTALTIDAWQDPEGRALTLRRAGPGLNGEVDVILIMMNGSSQALNFAAPAPHLE
                     WNVLMDSAAPDAPQYPLIGGELEVQAHSVAVLLAQPTGDADWQAVWMAGAHEGPRLLT
                     ALPPDPGTIGPRRTAEPWKAPIPAHAPPAKEEN"
     gene            686..2563
                     /gene="treZ"
                     /locus_tag="VHV99_30030"
     CDS             686..2563
                     /gene="treZ"
                     /locus_tag="VHV99_30030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016355342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HEX3639647.1"
                     /translation="MSETPIDPHKRHHAYCLPFGAHLTDAGHTRYRFWAPSLKAVQLE
                     LYPNKDGAAPTLIDMTPTADGWFEALAEGGAGTRYRYRINADLAVPDPVSRFQPDDVH
                     GPSEVIDPRAYRWENTNWHGRPWEETVLYEVHVGALGGYAGVTRRLKEFAHLGVTAIE
                     LMPLNDFSGSRNWGYDGVLPYAPDSAYGTPNELKSLIDAAHGLGLMVFLDVVYNHFGP
                     DGNYLSAYAKPFFRAGVNTPWGPAIDFERPQVRDFFFDNALYWLKEYRFDGLRLDAVH
                     AINDDEWLRELSERVRSSVEPLRHVHLVLENEHNTSNLLESHFNAQWSDDSHNTLHVL
                     LTGEDESYYGAYSDQPIQKLARVLSEGFVYQGEPSPIHDGKPRGEPSADLPPTAFVMF
                     LQNHDQVGNRAMGDRLRSLCSDDSLYATTGLMLLTPQIPMLFMEEQYGSTQPFLFFTD
                     YHDELADAVREGRRREFAKFSAFTDEKRRAQIPDPNAIETFRMSSPDASKQPADHNDW
                     LHFYRSALAVRTKLLMPRLKNAKTLGAKVIGEKAVLANWRLGDGSTYSVALNLGKEAI
                     PLTGHPPGKVVFETPARARDAADRGELGAETFIAWLTGDFADAAFNHDARRVKASGKT
                     S"
     gene            2560..4785
                     /gene="malQ"
                     /locus_tag="VHV99_30035"
     CDS             2560..4785
                     /gene="malQ"
                     /locus_tag="VHV99_30035"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008354018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEX3639648.1"
                     /translation="MSTTINTPATPDKTPGAIEALATKAGFEVEWQNAHKQIQRVPEE
                     TLKVLLERLDLPCGNATQLKQSAATLDAELSGRKLPPLMTAEVDRGISLPMAAVKSGA
                     RYRIELESGAVIDGRFTSPKGATALLSPISEPGYHTLVINEQRTTLAVAPARCYTVDD
                     AWRALQPSVQATPQATPPMWGVAAQVYGLRRVGDGGVGDFSALATLASESARRGSHAI
                     AISPMHAMFSAEPNKFSPYSPSSRLFLNIAHIDPAAVLGAQAAQVAIKTAGVADEFAE
                     LEALQLIDWPRVVTAKLAVLRALFAQFNAQTNDMLHKDFARFVKRGGRALEDHARFEA
                     LQAAQLKEASEGHWRNWAGELRDPRSEAVAEFAAANKEEVDFFLFAQWLAAKGLSHAQ
                     QVARDSGMAIGLIADLAVGCDSAGSHAWSYRDEMLQGVSVGAPPDLFNQAGQSWGLTT
                     FSPRAMRTQGFSAFIDMLRAAFTLAGGIRIDHILGLRRLWLVPDGESAKNGAYLRYPL
                     DDLLRLIALESWRHKAIVIGEDLGTVPPGFRERLHEHGLLGIRVLWFEHAEKGPGFKA
                     PADWDRDVAATTTTHDLPTVTGWWIGEDIVWRSKIGQTAARADGQNPVEMAQAERGED
                     RKLLWSAFQEAGVAAPDIPVSADISPQTAPVDEALAFVASTPAPLAIYPLEDLLALAE
                     MPNLPGSIDEHPNWRRRMAVPVSELFDDDSFVDRLLAIDQAREKSARTKAATHNASPT
                     D"
     gene            4790..>5366
                     /locus_tag="VHV99_30040"
     CDS             4790..>5366
                     /locus_tag="VHV99_30040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014251394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX3639649.1"
                     /translation="MTVPRSTLRLQLHKDFTFDDAARQVDYMAALGISHAYLSPITTA
                     TSGSTHGYDTVDYTRVNPELGGEEGLRRFVEKLRAHEMGVIVDVVPNHMGVGGSENLW
                     WMDILEWGRHAAHARYFDIDWHSPDPALRGKVLAPFLGAAYGEELQGGKIRLVFDAGQ
                     GRFKIEYYAHTFPVSPLDYTSVLQDHAPEMAQ"
BASE COUNT         1027 a         1740 c         1690 g          909 t
ORIGIN      
        1 cccgccggac gcgcgatgac agactttgtc gcccgcgtga tcgcgctgcg caaaaactac
       61 ccgttgctgc gggaaatgcg ttttctccac ggcgatcgcg aggtgctgcc aggtctttac
      121 gacgtcagct ggtttgacga aagcggcaca gcactgacta tcgacgcgtg gcaggacccc
      181 gaagggcgtg cgctgacgct gcgtcgcgcg gggcccggtc tgaacgggga agtcgatgtc
      241 atcctgatca tgatgaacgg atcgtcgcaa gcgctgaact tcgccgctcc cgcaccgcac
      301 ttggaatgga acgtgctcat ggatagcgct gcgcccgatg caccgcaata cccgctgatc
      361 ggcggagaac tggaagtgca ggcgcacagc gtagccgtac tgctcgcgca gccgaccggg
      421 gacgccgact ggcaggccgt ctggatggcc ggcgcccacg aagggccgcg cctcttgacg
      481 gcgttgccgc cggacccggg cacaatcgga ccacgacgga ccgcggaacc ctggaaggca
      541 cccatacccg cgcacgcgcc gcccgcaaag gaggaaaatt gaacggctcg aagacgccct
      601 gaagcggcac caaaacgtca ggcgcgaaac gccgggcagg ctccaagcca agtaactgac
      661 caagcgcact gaaggcacat acatcatgtc cgaaaccccg atcgatccgc acaagcgtca
      721 ccatgcctat tgcctgccct tcggcgctca cctcaccgat gccggacaca cgcgttatcg
      781 cttctgggcg ccgtcgctga aggcagtgca actggagctg tatcccaaca aagacggtgc
      841 ggctcccacg ctgatcgaca tgacgcccac cgccgacggc tggttcgaag cgctcgctga
      901 aggcggcgcg ggcacgcgtt atcggtatcg tatcaatgcg gatctggccg tgcccgatcc
      961 ggtctcgcgt ttccagcccg acgacgttca cggcccaagt gaggtgatcg acccgcgtgc
     1021 gtatcgttgg gaaaacacga actggcacgg ccggccgtgg gaagaaaccg tactttacga
     1081 agtgcacgtg ggcgcgctgg gcggttatgc cggcgtgacg cgccgcctga aggaattcgc
     1141 gcacctcggc gtgacggcga tcgaactgat gccgctgaac gacttcagcg gcagccgcaa
     1201 ctggggctac gacggcgtgc tgccctacgc gcccgattcg gcatacggca cgcccaatga
     1261 actgaagtcc ctcatcgacg ccgcgcatgg tctcggactg atggtcttcc tcgacgtggt
     1321 ttataaccat ttcggaccgg acggcaacta tctgagcgcc tacgccaagc cgttcttccg
     1381 cgcaggcgtg aatacgccgt ggggtccggc gatcgatttc gaacgtccgc aagtgcgcga
     1441 ctttttcttc gataacgcgc tgtactggct gaaggaatat cgcttcgacg ggctgcgcct
     1501 cgacgccgtc cacgccatca acgacgacga gtggttgcgc gaattgtcgg aacgcgtgcg
     1561 ttcatccgtg gaaccgctgc ggcacgtgca tcttgtgctg gagaacgagc acaacacgtc
     1621 gaacctgctc gaatcgcatt tcaacgcgca atggagcgac gactcgcaca acacgctgca
     1681 tgtcctgctg accggcgagg acgagagcta ttacggcgcg tacagcgacc agccaatcca
     1741 gaagcttgcg cgcgtgctga gcgagggctt cgtgtatcag ggcgagccct cgcccatcca
     1801 tgacggcaag ccgcgcggcg aaccgagcgc tgacttgccg cccacggcgt tcgtgatgtt
     1861 tttgcagaat cacgatcagg tcggcaaccg cgcaatgggc gaccgtttgc gctcgttgtg
     1921 cagcgacgac tcgctctacg ccaccaccgg cctgatgctc ctcacaccgc aaatcccgat
     1981 gctgttcatg gaagagcagt acggctccac gcagccattc ctgttcttca ccgactatca
     2041 cgacgaactt gccgacgctg tgcgcgaagg gcgtcgccgg gaatttgcga agttctccgc
     2101 cttcaccgac gaaaagcgcc gcgcgcagat tccggatccg aacgccatcg agacgttcag
     2161 gatgtcgtcg cccgatgcaa gcaagcagcc tgccgatcac aacgactggc tgcatttcta
     2221 tcgctcggcg ctggctgtgc gcacgaagct gctgatgccg cgcctgaaga atgcgaagac
     2281 gcttggcgcc aaggtgatcg gggagaaagc ggtgctggcg aactggcggc tcggcgatgg
     2341 cagcacctat tccgtcgcgc tgaacctggg caaggaagct attccactca ccggccatcc
     2401 gccgggcaaa gtcgtatttg aaacgccggc tcgggcccgc gacgcggcgg atcgcggcga
     2461 actgggtgca gagactttta tcgcgtggct gactggcgat ttcgccgatg ccgcgtttaa
     2521 tcacgacgcg cgtcgcgtca aagcttcggg gaaaacctcg tgagcacgac catcaatacg
     2581 ccggctacgc cggataaaac ccctggcgct atcgaggcgc tcgccaccaa agccggtttc
     2641 gaggttgagt ggcagaacgc gcacaagcag atccagcgcg tgccggaaga gacgctgaaa
     2701 gtgctgctgg aacgcctcga cctgccctgc ggcaacgcca cgcaactcaa gcagagcgcc
     2761 gcgacgctcg atgcggaatt gtccgggcgc aagttgccgc cgttgatgac cgcggaggtc
     2821 gatcgcggca tctcgctgcc gatggcggcc gtgaaatccg gcgcacggta tcgaatcgaa
     2881 ctcgaaagcg gcgcggttat cgacgggcga tttacgtcgc ccaaaggagc caccgccttg
     2941 ctctcgccga tctccgaacc gggctatcac acgctggtga tcaacgagca gcgtacgacg
     3001 ctcgcagtgg ccccggcgcg ctgctatacc gtcgacgatg cctggcgcgc gctccagccc
     3061 agcgtgcaag ccaccccgca ggccaccccg ccgatgtggg gcgtggccgc gcaggtatac
     3121 ggtttgcggc gcgtaggcga cggcggtgtg ggcgacttct ccgcgctcgc gacgcttgcg
     3181 tcggaaagcg cccggcgcgg cagccacgca atcgcgatca gcccgatgca cgccatgttc
     3241 agcgcagagc cgaacaaatt cagcccgtac tcgccttcat cgcgactgtt cctgaacatc
     3301 gcgcacatcg atccggctgc ggtgctgggc gcgcaagcgg cgcaggtggc gataaaaaca
     3361 gccggtgtcg ccgatgaatt cgccgaactc gaagcactgc agctgatcga ctggccgcgc
     3421 gtggtcacgg cgaaactggc cgtgctgcgc gcgttgttcg cgcagttcaa cgcgcagacc
     3481 aacgacatgc tgcacaagga cttcgcacgc ttcgtgaaac gcggcggacg cgcgctggaa
     3541 gatcacgccc gcttcgaagc gctgcaggcc gcgcaattga aggaagccag cgaaggccac
     3601 tggcgcaact gggccggcga actacgcgat ccgcgcagcg aggccgtcgc ggaattcgcg
     3661 gccgcgaaca aggaagaagt cgattttttc ctcttcgcgc aatggctcgc ggccaagggc
     3721 ttgtcgcatg cgcagcaggt cgcacgcgac tcgggcatgg cgattggctt gatagcagac
     3781 ttggccgtgg gctgcgacag cgcgggcagc cacgcgtggt cgtatcgcga cgaaatgctg
     3841 cagggtgtgt ccgtcggcgc gccgcccgat ctcttcaacc aggccggaca atcgtggggc
     3901 ctcaccacgt tttcaccccg cgccatgcgt acgcaaggct tctcggcgtt tatcgatatg
     3961 ctgcgtgccg cattcacgct cgcgggcggt atccgtatcg accacattct cggcttgcgg
     4021 cgtctctggc tcgtgcccga cggcgaaagc gcaaagaacg gcgcttacct gcgttatccg
     4081 ctcgacgacc tgctgcggct gatcgcgctc gaatcatggc ggcataaagc catcgtgatc
     4141 ggcgaggatc tcggcacggt tccgcccggc ttccgcgaac gcttgcacga acatggcctg
     4201 cttggcatcc gtgtgctctg gtttgaacac gccgaaaagg gtccgggctt caaggcgccc
     4261 gccgactggg atcgcgacgt ggccgcgacc accaccacgc atgacctgcc gacggttacc
     4321 ggctggtgga tcggcgagga cattgtgtgg cgctcgaaga tcggccagac ggcggcgcgt
     4381 gccgacggac agaatcctgt cgaaatggcc caggcggagc gcggcgagga tcgtaaattg
     4441 ctgtggtctg cctttcagga agccggtgtc gccgcgcccg acatccccgt gtccgccgat
     4501 atctccccgc aaacggcgcc ggtagacgaa gcgctcgcgt tcgtggcgtc cacgcccgcg
     4561 ccgcttgcga tctacccgct ggaagacttg ctcgcgctcg ccgagatgcc gaacctgccc
     4621 ggttccatcg acgagcatcc gaactggcgc cggcggatgg ctgttcccgt atccgaactc
     4681 tttgacgacg attcgttcgt cgaccgcctg cttgctatcg atcaggcccg cgaaaaatcc
     4741 gcccggacga aggcggccac gcacaacgct tcacccaccg actgagacca tgaccgttcc
     4801 acgctccacg ctccggctgc agctacacaa ggatttcacc ttcgacgacg ccgcgcgcca
     4861 ggtcgactac atggccgcgc tcggaatcag ccacgcgtat ttatcgccga tcacgaccgc
     4921 gaccagcggt tcgacgcacg gctacgacac ggtcgactac acccgtgtca atccggaact
     4981 cggcggtgaa gaagggttga ggcgtttcgt cgagaagctg cgcgcgcacg agatgggcgt
     5041 aatcgtggac gtggtgccga atcacatggg cgtgggcggt tcggaaaacc tctggtggat
     5101 ggatatcctc gaatggggac ggcacgcggc gcatgcgcgc tatttcgaca tcgactggca
     5161 ttcgcccgat cccgcgctgc gcggcaaagt gctcgcgccg ttcctgggcg cggcgtatgg
     5221 cgaggaattg cagggcggca agatcaggct ggtattcgac gccggccaag ggcgcttcaa
     5281 gatcgagtat tacgcgcaca cgtttccggt gagtccgctc gactacacgt cggtgttgca
     5341 ggaccacgcg cccgagatgg cgcagc
//
